bluuebunny/crossref_metadata_2025 huggingface dataset:
Outcomes of atherectomy in treating severely calcified coronary lesions in patients with reduced left ventricular ejection fraction: A systematic review and meta-analysis
Authors: Abusnina Waiel , Mostafa Mostafa Reda , Al-Abdouh Ahmad , Radaideh Qais , Ismayl Mahmoud , Alam Mahboob , Shah Jaffer , Yousfi Noraldeen El , Paul Timir K. , Ben-Dor Itsik , Dahal Khagendra
Published: September 2022

Background

Severely calcified coronary lesions with reduced left ventricular (LV) function result in worse outcomes. Atherectomy is used in treating such lesions when technically feasible. However, there is limited data examining the safety and efficacy of atherectomy without hemodynamic support in treating severely calcified coronary lesions in patients with reduced left ventricular ejection fraction (LVEF).

Objective

To evaluate the clinical outcomes of atherectomy in patient with reduced LVEF.

Methods

We searched PubMed, Cochrane CENTRAL Register and ClinicalTrials.gov (inception through July 21, 2021) for studies evaluating the outcomes of atherectomy in patients with severe LV dysfunction. We used random-effect model to calculate risk ratio (RR) with 95% confidence interval (CI). The endpoints were in-hospital and long term all-cause mortality, cardiac death, myocardial infarction (MI), and target vessel revascularization (TVR).

Results

A total of 7 studies consisting of 2,238 unique patients were included in the analysis. The median follow-up duration was 22.4 months. The risk of in-hospital all-cause mortality using atherectomy in patients with severely reduced LVEF compared to the patients with moderate reduced or preserved LVEF was [2.4vs.0.5%; RR:5.28; 95%CI 1.65–16.84; P = 0.005], the risk of long term all-cause mortality was [21 vs. 8.8%; RR of 2.84; 95% CI 1.16–6.95; P = 0.02]. In-hospital TVR risk was 2.0 vs. 0.6% (RR: 4.15; 95% CI 4.15–15.67; P = 0.04) and long-term TVR was [6.0 vs. 9.9%; RR of 0.75; 95% CI 0.39–1.42; P = 0.37]. In-hospital MI was [7.1 vs. 5.4%; RR 1.63; 95% CI 0.91–2.93; P = 0.10], long-term MI was [7.5 vs. 5.7; RR 1.74; 95%CI 0.95–3.18; P = 0.07).

Conclusion

Our meta-analysis suggested that the patients with severely reduced LVEF when using atherectomy devices experienced higher risk of clinical outcomes in the terms of all-cause mortality and cardiac mortality. As we know that the patients with severely reduced LVEF are inherently at increased risk of adverse clinical outcomes, this information should be considered hypothesis generating and utilized while discussing the risks and benefits of atherectomy in such high risk patients. Future studies should focus on the comparison of outcomes of different atherectomy devices in such patients. Adjusting for the inherent mortality risk posed by left ventricular dysfunction may be a strategy while designing a study.
Cardiac MRF using rosette trajectories for simultaneous myocardial T1, T2, and proton density fat fraction mapping
Authors: Liu Yuchi , Hamilton Jesse , Jiang Yun , Seiberlich Nicole
Published: September 2022

The goal of this work is to extend prior work on cardiac MR Fingerprinting (cMRF) using rosette k-space trajectories to enable simultaneous T1, T2, and proton density fat fraction (PDFF) mapping in the heart. A rosette trajectory designed for water-fat separation at 1.5T was used in a 2D ECG-triggered 15-heartbeat cMRF sequence. Water and fat specific T1 and T2 maps were generated from the cMRF data. A PDFF map was also retrieved using Hierarchical IDEAL by segmenting the rosette cMRF data into multiple echoes. The accuracy of rosette cMRF in T1, T2, and PDFF quantification was validated in the ISMRM/NIST phantom and an in-house built fat fraction phantom, respectively. The proposed method was also applied for myocardial tissue mapping of healthy subjects and cardiac patients at 1.5T. T1, T2, and PDFF values measured using rosette cMRF in the ISMRM/NIST phantom and the fat fraction phantom agreed well with the reference values. In 16 healthy subjects, rosette cMRF yielded T1 values which were 80~90 ms higher than spiral cMRF and MOLLI. T2 values obtained using rosette cMRF were ~3 ms higher than spiral cMRF and ~5 ms lower than conventional T2-prep bSSFP method. Rosette cMRF was also able to detect abnormal T1 and T2 values in cardiomyopathy patients and may provide more accurate maps due to effective fat suppression. In conclusion, this study shows that rosette cMRF has the potential for efficient cardiac tissue characterization through simultaneous quantification of myocardial T1, T2, and PDFF.
Reservoir characteristics and factors influencing shahejie marl in the shulu sag, bohai bay basin, eastern China
Authors: Li Jiajing , Fu Guang , Zhu Douxing , Cao Lanzhu , Li Zhaolong , Lv Yanfang , Li Wenke , Hu Ming , Liu Zhe
Published: September 2022

Shahejie marl in the Shulu Sag is a crucial resource for unconventional hydrocarbon exploration in China. Although breakthroughs have been made in tight oil exploration in this area, the mechanisms underlying the formation of this marl reservoir and factors controlling its ‘sweet spots’ have not been thoroughly studied. To understand the pore structure characteristics and factors influencing the marl reservoir, we analyzed core samples from Wells ST1 and ST3. A series of experiments was conducted on the samples, such as X-ray diffraction, focused ion beam scanning electron microscopy, micro-CT, and total organic carbon test. Additionally, the physical properties of different marl rock fabrics were studied with auxiliary tests, such as mercury intrusion capillary pressure analyses, nuclear magnetic resonance, porosity and permeability tests, and thin-section observation. The results revealed that the marl reservoir is characterized by low porosity (1.61%) and low permeability (2.56mD). The porosity and permeability (1.61% and 3.26mD) of laminated marl were better than those (0.92% and 1.68mD) of massive marl. Clay minerals and quartz content in laminated (11.8 and 8.2%) was less than in massive marl (16.2 and 13.3%). The marl pores include intercrystalline pores, dissolution pores, and microfractures. Additionally, the laminated marl pores were primarily distributed along the dark lamina, with good connectivity. A few isolated and uniform holes were observed in the massive marl. Influenced by rock fabric and mineral composition, layered fractures were mainly developed in the laminated marl, while structural fractures were the main type of microfractures in the massive marl. The primary sedimentary mechanism was the main geological action underlying the differences in marl rock fabric; this mechanism affects the physical properties of the marl reservoir, which are key factors to be considered when searching for the marl reservoir ‘sweet spots’. Particular attention should be paid to these factors during tight oil exploration and development in similar sedimentary basins.
Simulation of tectonic stress field and prediction of tectonic fracture distribution in Longmaxi Formation in Lintanchang area of eastern Sichuan Basin
Authors: Zhang Douzhong , Tang Jiguang , Chen Kongquan , Wang Kaiming , Zhang Peixian , He Guisong , Tuo Xiusong
Published: September 2022

Shale is a low-porosity and low-permeability reservoir, and structural fractures are the main controlling factor for the migration and accumulation of shale gas. Moreover, tectonic fractures are controlled by the paleo-tectonic stress field. In this paper, taking the Longmaxi Formation of the Lintanchang area as an example, the finite element numerical simulation technology is used to analyze the distribution law of the paleo-tectonic stress field, and further, the fracture development areas under the superposition of two periods of tectonic stress are predicted using seismic, rock mechanics, and field data. The results show that the tectonic fractures developed in the Lintanchang area are mainly EW- and NNW-striking conjugate shear fractures formed in the Mid-Yanshanian period, followed by the NWW- and SWW-striking conjugate shear fractures formed in the late Yanshanian period. The distribution of tectonic fractures is affected by faults, folds, rock physical parameters and tectonic stresses. It is found that the comprehensive fracture coefficients of the anticline core and fault areas are both greater than 1.1, which are the areas with the most developed structural fractures, and these areas have poor shale gas preservation conditions. However, the comprehensive fracture coefficients of the western flanks of the anticline and the eastern and western dipping ends are between 1.0 and 1.1, which are areas with better shale gas preservation conditions. In addition, the development degree of tectonic fractures in the east and northwest areas of the Lintanchang anticline is lower than that in other areas. The comprehensive fracture coefficients of shale in these areas are between 0.9 and 1.0. The shale is in a state of “breaking without cracking”, and shale gas can be well preserved.
Social Media Analytics by Virtual Operations Support Teams in disaster management: Situational awareness and actionable information for decision-makers
Authors: Fathi Ramian , Fiedrich Frank
Published: September 2022

Virtual Operations Support Teams are groups of institutionalized digital volunteers in the field of disaster management who conduct Social Media Analytics tasks for decision-makers in Emergency Operation Centers (EOCs) during hazard situations such as floods. Through interagency integration into EOC structures, the volunteers provide analytical support using advanced tools and monitoring various social media platforms. The goal of VOSTs is to increase decision-makers’ situational awareness through need-oriented analysis and to improve decision-making by providing actionable information in a time-critical work context. In this case study, the data collected during the 2021 flood in Wuppertal, Germany by 22 VOST analysts was processed and analyzed. It was found that information from eight social media platforms could be classified into 23 distinct categories. The analysts’ prioritizations indicate differences in the formats of information and platforms. Disaster-related posts that pose a threat to the affected population’s health and safety (e.g., requests for help or false information) were more commonly prioritized than other posts. Image-heavy content was also rated higher than text-heavy data. A subsequent survey of EOC decision-makers examined the impact of VOST information on situational awareness during this flood. It also asked how actionable information impacted decisions. We found that VOST information contributes to expanded situational awareness of decision-makers and ensures people-centered risk and crisis communication. Based on the results from this case study, we discuss the need for future research in the area of integrating VOST analysts in decision-making processes in the field of time-critical disaster management.
Interpersonal and academic self-efficacy and its relationship with employment of food industry engineering students: A gender perspective
Authors: Reyes-González Ana M. , Velázquez-Sánchez Luz M. , Rojas-Parra Azucena , Chuck-Hernández Cristina
Published: September 2022

An analysis of self-efficacy and its effect on employment was performed with data obtained from Food Industry Engineering Students (FIES), with strong food engineering, food manufacturing and innovation-related competencies, from 2019 to 2021. The aim was to determine if there is a relationship between self-efficacy and employment in FIES to define potential actions for educational institutions looking to reduce the gender disparity in professional life. Results showed differences in self-assessment categories regarding gender, with men having a better self-perception, especially in economic analysis and clarity in professional objectives. Women rate higher only in food development, traditionally associated with women from household to the food industry. Except for the entrepreneur positions favoring men, these differences were not observed in the employment data 1 year after graduation. This might be due to the national context where entrepreneurship is associated with masculine traits. Tasks commonly associated with specific genders seem to influence student self-efficacy, but this is not reflected in the employment rate of FIES at least within the evaluated period. Food Industry Engineering is considered atypical within STEM disciplines because more women graduate than men depicting gender roles similar to those in the national environment. These results are important for employers, government, and higher education institutions to create strategies to improve self-efficacy among students to reach their full potential as employees and as entrepreneurs to create a better environment for national economic development.
Duration of oral contraceptive use relates to cognitive performance and brain activation in current and past users
Authors: Noachtar Isabel Asar , Hidalgo-Lopez Esmeralda , Pletzer Belinda
Published: September 2022

Previous studies indicate effects of oral contraceptive (OC) use on spatial and verbal cognition. However, a better understanding of the OC effects is still needed, including the differential effects of androgenic or anti-androgenic OC use and whether the possible impact persists beyond the OC use. We aim to investigate the associations of OC use duration with spatial and verbal cognition, differentiating between androgenic and anti-androgenic OC. Using functional magnetic resonance imaging (MRI), we scanned a group of 94 past and current OC-users in a single session. We grouped current OC users (N=53) and past OC users with a natural cycle (N=41) into androgenic and anti-androgenic user. Effects of OC use duration were observed for current use and after discontinuation. Duration of OC use was reflected only in verbal fluency performance but not navigation: The longer the current OC use, the less words were produced in the verbal fluency task. During navigation, deactivation in the caudate and postcentral gyrus was duration-dependent in current androgenic OC users. Only during the verbal fluency task, duration of previous OC use affects several brain parameters, including activation of the left putamen and connectivity between right-hemispheric language areas (i.e., right inferior frontal gyrus and right angular gyrus). The results regarding performance and brain activation point towards stronger organizational effects of OCs on verbal rather than spatial processing. Irrespective of the task, a duration-dependent connectivity between the hippocampus and various occipital areas was observed. This could suggest a shift in strategy or processing style with long-term contraceptive use during navigation/verbal fluency. The current findings suggest a key role of the progestogenic component of OCs in both tasks. The influence of OC use on verbal fluency remains even after discontinuation which further points out the importance of future studies on OC effects and their reversibility.
Is intracrinology of endometriosis relevant in clinical practice? A systematic review on estrogen metabolism
Authors: Mercorio Antonio , Giampaolino Pierluigi , Romano Andrea , Dällenbach Patrick , Pluchino Nicola
Published: September 2022

Endometriosis is a chronic, multifactorial, estrogen-dependent disease. The abnormal endocrine microenvironment of endometriosis lesions is considered a main feature and multiple enzymatic pathways leading to local increased synthesis of estrogens have been identified. However, the relevance of intracrinology in clinical practice is still lacking. Medline, Embase, Scopus database were systematically searched for studies reporting on local estrogens metabolism of endometriotic lesions. The main enzymatic pathways involved in the intracrinology of endometriosis such as aromatase (CYP19A1), 17β-hydroxysteroid dehydrogenase (HSD17B) type 1, type 2 and type 5, steroid sulfatase (STS), estrogen sulfotransferase (SULT1E1) were assessed with a critical perspective on their role in disease endocrine phenotyping, drug resistance and as therapeutic targets. Overall, studies heterogeneity and missing clinical data affect the interpretation of the clinical role of these enzymes. Although the use of some drugs such as aromatase inhibitors has been proposed in clinical practice for two decades, their potential clinical value is still under investigation as well as their modality of administration. A closer look at new, more realistic drug targets is provided and discussed. Altered expression of these key enzymes in the lesions have far reaching implication in the development of new drugs aimed at decreasing local estrogenic activity with a minimal effect on gonadal function; however, given the complexity of the evaluation of the expression of the enzymes, multiple aspects still remains to be clarified.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022311329, identifier CRD42022311329.
Regulation and bioinformatic analysis of circ_0015891/miR-129-1-3p axis in methamphetamine-induced dopaminergic apoptosis
Authors: Deng Bingpeng , Tang Xuan , Wang Yong
Published: September 2022

Methamphetamine (METH) abuse can result in severe neurotoxicity, for which the mechanism is not yet clear. In the present study, we investigated the role of noncoding RNAs in METH-induced dopaminergic neurotoxicity, and analyzed the underlying mechanism using bioinformatic methods. We confirmed by flow cytometry that miR-129-1-3p is involved in promoting dopaminergic apoptosis under METH treatment and its role could be inhibited by a high concentration of circ_0015891. Also, we combined transcriptomic data with bioinformatics to explore the downstream mechanism of miR-129-1-3p regulation of METH-induced apoptosis, highlighted the potentially pivotal figure of response to nutrition. Further bioinformatic analysis of circ_0015891 was conducted as well and showed that circ_0015891 was the sponge of various microRNAs that effect apoptosis by different mechanisms. Collectively, we found a novel circ_0015891/miR-129-1-3p axis that may be a promising therapeutic target for METH-induced dopaminergic neurotoxicity.
Low carbon economic dispatch of power system at multiple time scales considering GRU wind power forecasting and integrated carbon capture
Authors: Ding Can , Zhou Yiyuan , Pu Guang , Zhang Hongrong
Published: September 2022

To achieve carbon neutrality in electricity, measures such as increasing the share of renewable energy sources such as wind power and achieving more accurate and faster wind power forecasting, and low carbon retrofitting of thermal power units are all important to achieve the goal. Firstly, the GRU prediction algorithm was used to forecast wind power, which performed well in terms of prediction accuracy and model training speed. Then, we continue to fully utilize the source-side low-carbon characteristics by installing flue gas bypass systems and liquid storage in carbon capture plants to form an integrated carbon capture plant operation, thereby reducing carbon emissions and the proportion of abandoned wind. Secondly, a three-stage low carbon economic dispatch model is established to reduce wind abandonment by combining wind power forecasts on different time scales. Finally, a case study was carried out using a modified IEEE-39 node system. The results show that the proposed three-stage integrated dispatching method can make full use of wind energy and achieve the goal of economic dispatching of the power system.
Forecast calibrations of surface air temperature over Xinjiang based on U-net neural network
Authors: Zhu Yanhe , Zhi Xiefei , Lyu Yang , Zhu Shoupeng , Tong Hua , Mamtimin Ali , Zhang Hailiang , Huo Wen
Published: September 2022

In this study, a deep learning method named U-net neural network is utilized to calibrate the gridded forecast of surface air temperature from the Global Ensemble Forecasting System (GEFS), with forecast lead times of 1–7 days in Xinjiang. The calibration performance of U-net is compared with three conventional postprocessing methods: unary linear regression (ULR), the decaying averaging method (DAM) and Quantile Mapping (QM). Results show that biases of the raw GEFS forecasts are mainly distributed in the Altai Mountains, the Junggar Basin, the Tarim Basin and the Kunlun Mountains. The four postprocessing methods effectively improve the forecast skills for all lead times, whereas U-net shows the best correction performance with the lowest mean absolute error (MAE) and the highest hit rate of 2°C (HR2) and pattern correlation coefficient (PCC). The U-net model considerably reduces the warm biases of the raw forecasts. The skill improvement magnitudes are greater in southern than northern Xinjiang, showing a higher mean absolute error skill score (MAESS). Furthermore, in order to distinguish the error sources of each forecasting scheme and to reveal their capabilities of calibrating errors of different sources, the error decomposition analysis is carried out based on the mean square errors. It shows that the bias term is the leading source of error in the raw forecasts, and barely changes as the lead time increases, which is mainly distributed in Tarim Basin and Kunlun Mountains. All four forecast calibrations effectively reduce the bias and distribution error of the raw forecasts, but only the U-net significantly reduces the sequence error.
The research on the effect of digital economy development on urban air quality
Authors: Song Baolin , Shi Haozhe , Wang Mengxin , Gu Ran
Published: September 2022

Digital economy and air quality are the key issues concerned by the government and academia. The healthy and sustainable development of the digital economy and the continuous optimization of urban air quality are not only conducive to high-quality economic development but also closely related to people’s livelihood. Based on the panel data of 228 cities from 2015 to 2020, using the panel regression and the mediating effect test methods, this paper verifies the impact of the digital economy development on urban air quality and then further analyzes the heterogeneity. The main results are as follows. Digital economy development can effectively improve urban air quality. The mediating effect of the urban industrial structure advancement accounts for 17.27%, and that of urban TFP accounts for 14.55%. The effect of improving air quality in cities with a high-level digital economy is more prominent, and the effect in large and medium-sized cities is more extensive. Meanwhile, in cities with a high urbanization rate, the effect of digital economy on improving air quality is more prominent.
Urban gulls show similar thermographic and behavioral responses to human shouting and conspecific alarm calls
Authors: Di Giovanni Julien , Fawcett Tim W. , Templeton Christopher N. , Raghav Shubhi , Boogert Neeltje J.
Published: September 2022

Rapid population growth and the urbanization of modern environments are markedly increasing human-wildlife conflict. Wild animals in urban landscapes can benefit from exploiting human resources, but are also exposed to increased risk of human-caused injury, which should favor the ability to perceive and respond to human cues. Although it is well known that domesticated animals use human cues that may indicate threats, less is known about wild animals living in urban environments. Herring gulls (Larus argentatus) in urban landscapes have adapted kleptoparasitic behaviors to obtain human food, often resulting in negative interactions with humans. Here we quantified both the behavioral and physiological responses of free-living urban herring gulls to human shouting. We presented urban gulls with a fake human food item and played back recordings of either a man shouting, a natural stressor (i.e., conspecific alarm call), or a neutral stimulus (i.e., robin song). We recorded behavioral responses and used non-invasive infrared thermography to measure eye-region surface temperature changes associated with the avian physiological stress response. We found that gulls exposed to shouting and to conspecific alarm calls showed similar changes in behavior (indicating high levels of vigilance) and eye-region surface temperature (indicating physiological stress). Both responses were significantly stronger than the responses to robin song. Additionally, the behavioral and physiological responses were positively correlated across individuals. Our results demonstrate that urban-dwelling gulls respond to human shouting and conspecific alarm calls in a similar way, and suggest that infrared thermography is a viable technique to monitor stress responses in free-living birds.
Vici syndrome in Israel: Clinical and molecular insights
Authors: Chorin Odelia , Hirsch Yoel , Rock Rachel , Salzer Sheelo Liat , Goldberg Yael , Mandel Hanna , Hershkovitz Tova , Fleischer Nicole , Greenbaum Lior , Katz Uriel , Barel Ortal , Hamed Nasrin , Ben-Zeev Bruria , Greenberger Shoshana , Nasser Samra Nadra , Stern Zimmer Michal , Raas-Rothschild Annick , Pode-Shakked Ben
Published: September 2022

Introduction: Vici Syndrome is a rare, severe, neurodevelopmental/neurodegenerative disorder with multi-systemic manifestations presenting in infancy. It is mainly characterized by global developmental delay, seizures, agenesis of the corpus callosum, hair and skin hypopigmentation, bilateral cataract, and varying degrees of immunodeficiency, among other features. Vici Syndrome is caused by biallelic pathogenic variants in EPG5, resulting in impaired autophagy. Thus far, the condition has been reported in less than a hundred individuals.

Objective and Methods: We aimed to characterize the clinical and molecular findings in individuals harboring biallelic EPG5 variants, recruited from four medical centers in Israel. Furthermore, we aimed to utilize a machine learning-based tool to assess facial features of Vici syndrome.

Results: Eleven cases of Vici Syndrome from five unrelated families, one of which was diagnosed prenatally with subsequent termination of pregnancy, were recruited. A total of five disease causing variants were detected in EPG5: two novel: c.2554-5A>G and c.1461delC; and 3 previously reported: c.3447G>A, c.5993C>G, and c.1007A>G, the latter previously identified in several patients of Ashkenazi-Jewish (AJ) descent. Amongst 140,491 individuals screened by the Dor Yeshorim Program, we show that the c.1007A>G variant has an overall carrier frequency of 0.45% (1 in 224) among AJ individuals. Finally, based on two-dimensional facial photographs of individuals with Vici syndrome (n = 19), a composite facial mask was created using the DeepGestalt algorithm, illustrating facial features typical of this disorder.

Conclusion: We report on ten children and one fetus from five unrelated families, affected with Vici syndrome, and describe prenatal and postnatal characteristics. Our findings contribute to the current knowledge regarding the molecular basis and phenotypic features of this rare syndrome. Additionally, the deep learning-based facial gestalt adds to the clinician’s diagnostic toolbox and may aid in facilitating identification of affected individuals.
Bidirectional causal link between inflammatory bowel disease and celiac disease: A two-sample mendelian randomization analysis
Authors: A Gu , Sun Caixia , Shan Yuezhan , Husile Husile , Bai Haihua
Published: September 2022

Background: Observational research has shown a correlation between inflammatory bowel disease (IBD) [comprising ulcerative colitis (UC) and Crohn’s disease (CD)] and celiac disease. However, the relationship between these two diseases remains uncertain.

Methods: We utilized two-sample Mendelian randomization (MR) to estimate the bidirectional causal relationships between IBD and celiac disease. This study utilized data on single nucleotide polymorphisms (SNPs) from genome-wide association studies (GWASs). Heterogeneity, pleiotropy, and sensitivity analyses were also performed to evaluate the MR results.

Results: There was a significant causal relationship between IBD and CD and celiac disease (e.g., IBD and celiac disease, inverse variance weighting (IVW) odds ratio (OR) = 1.0828, 95% CI = 1.0258–1.1428, p = 0.0039; CD and celiac disease, IVW OR = 1.0807, 95% CI = 1.0227–1.1420, p = 0.0058). However, in the reverse direction, we found only suggestive positive causality between celiac disease and CD (e.g., IVW OR = 1.0366, 95% CI = 1.0031–1.0711, p = 0.0319). No evidence of heterogeneity between genetic variants was found (e.g., IBD vs. celiac disease, MR-Egger Q = 47.4391, p = 0.6159). Horizontal pleiotropy hardly influenced causality (e.g., IBD vs. celiac disease, MR-Egger test: p = 0.4340). Leave-one-out analysis showed that individual SNPs did not influence the general results.

Conclusion: Our MR analysis revealed a positive causal link between IBD and celiac disease in the European population. In addition, several recommendations for disease prevention and clinical management have been discussed.
Potential of conserved antigenic sites in development of universal SARS-like coronavirus vaccines
Authors: Wang Siling , Wu Dinghui , Xiong Hualong , Wang Juan , Tang Zimin , Chen Zihao , Wang Yizhen , Zhang Yali , Ying Dong , Lin Xue , Liu Chang , Guo Shaoqi , Tian Weikun , Lin Yajie , Zhang Xiaoping , Yuan Quan , Yu Hai , Zhang Tianying , Zheng Zizheng , Xia Ningshao
Published: September 2022

Given pandemic risks of zoonotic SARS-CoV-2 variants and other SARS-like coronaviruses in the future, it is valuable to perform studies on conserved antigenic sites to design universal SARS-like coronavirus vaccines. By using antibodies obtained from convalescent COVID-19 patients, we succeeded in functional comparison of conserved antigenic sites at multiple aspects with each other, and even with SARS-CoV-2 unique antigenic sites, which promotes the cognition of process of humoral immune response to the conserved antigenic sites. The conserved antigenic sites between SARS-CoV-2 and SARS-CoV can effectively induce affinity maturation of cross-binding antibodies, finally resulting in broadly neutralizing antibodies against multiple variants of concern, which provides an important basis for universal vaccine design, however they are subdominant, putatively due to their lower accessibility relative to SARS-CoV-2 unique antigenic sites. Furthermore, we preliminarily design RBDs to improve the immunogenicity of these conserved antigenic sites. Our study focusing on conserved antigenic sites provides insights for promoting the development of universal SARS-like coronavirus vaccines, thereby enhancing our pandemic preparedness.
Assessing the importance of kelp forests for small-scale fisheries under a global change scenario
Authors: Piñeiro-Corbeira Cristina , Barrientos Sara , Barreiro Rodolfo , de la Cruz-Modino Raquel
Published: September 2022

Kelp forests are critical habitats for temperate coasts that are experiencing dramatic declines worldwide in recent decades. Yet, even though they often support wildlife populations of high socioeconomic value, the consequences of kelp forest decline for small-scale fisheries (SSFs) have received surprisingly little attention. Here, we take the first step to fill this gap through the local ecological knowledge (LEK) of SSF fishers whose fisheries are associated with this habitat in NW Spain. LEK was used to 1) estimate kelp forest loss, 2) identify the main fisheries associated with kelp forests, 3) gain insight into the changes these fisheries may have undergone in recent times, 4) evaluate the economic importance of kelp in the study area, and 5) describe the commercial chain of exploited kelps and relevant fisheries related to them. Fisher’s knowledge of kelp forests was documented through interviews with the help of a semi-structured questionnaire with open–closed questions about the small-scale fishery and its target species. Additionally, participants were asked to map the current and former (20 years ago) distribution of kelp forests in their fishing area. Results show that a range of fish, crustaceans, mollusks, echinoderms, and even the kelp itself are fished/harvested in the study area, suggesting the socioeconomic value of those species. The most intensively targeted species usually belong to fisheries with high commercial value, and first-sale data indicate that they are worth some 10 million euros to the local economy. On the other hand, compared to two decades ago, fishers reported a substantial contraction in the area occupied by kelp forests and decreases in the fisheries typically associated with this habitat. Landing data partly support this perception of a decline in catches. Altogether, this information will be useful to foster kelp forest conservation and to evaluate their socioecological and economic implications for SSFs.
A framework for inland cities to prevent marine debris: A case study from Durham, North Carolina
Authors: Lauer Nancy E. , Nowlin Michelle B.
Published: September 2022

Land-based sources of litter are increasingly recognized as significant contributors to marine debris, and rivers can carry debris to the coast from far-inland sources. In this paper, we demonstrate the important role inland cities can play in the marine debris crisis by reducing their own marine debris contributions. Given this role, we provide a framework for inland cities to prevent plastic pollution along with the lessons learned from introducing these strategies in Durham, North Carolina, a mid-sized, inland city that drains to the ocean through the Cape Fear and Neuse River watersheds. This framework guides city officials, resource managers, and community partners on how to characterize the plastic pollution problem in their city by collecting baseline data on plastic waste and litter. This framework also provides practical and equitable solutions for inland cities to address plastic pollution. We recommend that inland cities prioritize policy solutions that reduce waste at the source – to the extent that their state constitutions allow – and to also use authorities for stormwater controls to capture and remove debris as long as litter persists. Replicating this framework in other inland cities opens vast opportunities to manage and reduce marine debris from an often-overlooked source.
Optimized uncertainty propagation across high fidelity taylor anvil simulation
Authors: James Jaylen R. , Sanghvi Meet , Gerlt Austin R. C. , Allaire Douglas , Arroyave Raymundo , Gonzales Manny
Published: September 2022

In computational materials research, uncertainty analysis (more specifically, uncertainty propagation, UP) in the outcomes of model predictions is essential in order to establish confidence in the models as well as to validate them against the ground truth (experiments or higher fidelity simulations). Unfortunately, conventional UP models relying on exhaustive sampling from the distributions of input parameters may be impractical, particularly when the models are computationally expensive. In these cases, investigators must sacrifice accuracy in the propagated uncertainty by down-sampling the input distribution. Recently, a method was developed to correct for these inaccuracies by re-weighing the input distributions to create more statistically representative samples. In this work, the method is applied to computational models for the response of materials under high strain rates. The method is shown to effectively approximate converged output distributions at a lower cost than using conventional sampling approaches.
The influence of curing temperature on the strength and phase assemblage of hybrid cements based on GGBFS/FA blends
Authors: Henning Ricky , Sturm Patrick , Geddes Daniel A. , Keßler Sylvia , Walkley Brant , Gluth Gregor J. G.
Published: September 2022

Hybrid cements are composites made of Portland cement or Portland clinker and one or more supplementary cementitious materials like slag, fly ash or metakaolin, activated with an alkali salt. To date, their hydration mechanism and the phase formation at various temperatures is insufficiently understood, partly due to the large variability of the raw materials used. In the present study, three hybrid cements based on ground granulated blast furnace slag, fly ash, Portland clinker and sodium sulfate, and an alkali-activated slag/fly ash blend were cured at 10 and 21.5°C, and subsequently analyzed by XRD, 27Al MAS NMR, and TGA. The compressive strength of the hybrid cements was higher by up to 27% after 91-day curing at 10°C, compared to curing at 21.5°C. The experimental results as well as thermodynamic modeling indicate that the differences in compressive strength were related to a different phase assemblage, mainly differing amounts of strätlingite and C-N-A-S-H, and the associated differences of the volume of hydration products. While the strätlingite was amorphous to X-rays, it could be identified by 27Al MAS NMR spectroscopy, TGA and thermodynamic modeling. The microstructural properties of the hybrid cements and the alkali-activated slag/fly ash blend as well as the compatibility between thermodynamic modeling results and experimental data as a function of curing temperature and time are discussed.
Regulation of lipid droplet (LD) formation in hepatocytes via regulation of SREBP1c by non-coding RNAs
Authors: El Sobky Shereen A. , Aboud Nourhan K. , El Assaly Nihal M. , Fawzy Injie O. , El-Ekiaby Nada , Abdelaziz Ahmed I.
Published: September 2022

Introduction

Increased de novo lipogenesis (DNL) is one of the key factors contributing to fat accumulation and non-alcoholic fatty liver disease (NAFLD). Among the critical transcription factors (TFs) regulating DNL is mTOR and its downstream lipogenic TF, SREBP1c. In recent years, it has been established that non-coding RNAs (ncRNAs) play role in both biological processes and disease pathogenesis. Our group has previously characterized microRNAs that can target and regulate the expression of both mTOR and SREBP1c. Accordingly, this study aimed to broaden our understanding of the role of ncRNAs in regulating the mTOR/SREBP1c axis to elucidate the role of the non-coding transcriptome in DNL and lipid droplet (LD) formation. Hence, short ncRNA, miR-615-5p, and long non-coding RNA (lncRNA), H19, were chosen as they were previously proven to target mTOR by our group and in the published literature, respectively.

Methodology

Huh-7 cells were treated with 800 μM oleic acid (OA) to promote LD formation. Transfection of miR-615-5p mimics or H19 over-expression vectors was performed, followed by the measurement of their downstream targets, mTOR and SREBP, on the mRNA level by quantitative real-time PCR (qRT-PCR), and on the protein level by Western blot. To determine the functional impact of miR-615-5p and H19 on LD formation and triglyceride (TG) accumulation, post-transfection LDs were stained, imaged, and characterized, and TGs were extracted and quantified.

Results

miR-615-5p was able to reduce mTOR and SREBP1c significantly on both the mRNA and protein levels compared to control cells, while H19 caused a reduction of both targets on the protein level only. Both miR-615-5p and H19 were able to significantly reduce the LD count and total area, as well as TG levels compared to control cells.

Conclusion

To conclude, this study shows, for the first time, the impact of miR-615-5p and H19 on the mTOR/SREBP1c axis, and thus, their functional impact on LDs and TG accumulation. These findings might pave the way for using ncRNAs as potential therapeutic targets in the management of fatty liver.
Endothelial senescence mediates hypoxia-induced vascular remodeling by modulating PDGFB expression
Authors: Kyi Priscilla , Hendee Kathryn , Hunyenyiwa Tendai , Matus Kienna , Mammoto Tadanori , Mammoto Akiko
Published: September 2022

Uncontrolled accumulation of pulmonary artery smooth muscle cells (PASMCs) to the distal pulmonary arterioles (PAs) is one of the major characteristics of pulmonary hypertension (PH). Cellular senescence contributes to aging and lung diseases associated with PH and links to PH progression. However, the mechanism by which cellular senescence controls vascular remodeling in PH is not fully understood. The levels of senescence marker, p16INK4A and senescence-associated β-galactosidase (SA-β-gal) activity are higher in PA endothelial cells (ECs) isolated from idiopathic pulmonary arterial hypertension (IPAH) patients compared to those from healthy individuals. Hypoxia-induced accumulation of α-smooth muscle actin (αSMA)-positive cells to the PAs is attenuated in p16fl/fl-Cdh5(PAC)-CreERT2 (p16iΔEC) mice after tamoxifen induction. We have reported that endothelial TWIST1 mediates hypoxia-induced vascular remodeling by increasing platelet-derived growth factor (PDGFB) expression. Transcriptomic analyses of IPAH patient lungs or hypoxia-induced mouse lung ECs reveal the alteration of senescence-related gene expression and their interaction with TWIST1. Knockdown of p16INK4A attenuates the expression of PDGFB and TWIST1 in IPAH patient PAECs or hypoxia-treated mouse lungs and suppresses accumulation of αSMA–positive cells to the supplemented ECs in the gel implanted on the mouse lungs. Hypoxia-treated mouse lung EC-derived exosomes stimulate DNA synthesis and migration of PASMCs in vitro and in the gel implanted on the mouse lungs, while p16iΔEC mouse lung EC-derived exosomes inhibit the effects. These results suggest that endothelial senescence modulates TWIST1-PDGFB signaling and controls vascular remodeling in PH.
Determining the center of a keratoconus: Comparison of different tomographic parameters and impact of disease severity
Authors: Steinwender Gernot , Kollenc Alexander , Shajari Mehdi , Sommer Michael , Borenich Andrea , Horwath-Winter Jutta , Lindner Ewald , Woltsche Nora , List Wolfgang , Wedrich Andreas
Published: September 2022

Purpose

There exists remarkable variation in definitions for the location of the center of a keratoconus. The objective of this study was to analyze deviations between locations obtained by different tomographic maps for that purpose. Furthermore, it was investigated whether these deviations are influenced by disease severity.

Methods

In 162 eyes with keratoconus, corneal tomographic maps derived by Scheimpflug technology were retrospectively analyzed to determine the cone location with 5 different methods: maximum axial curvature of the front surface (Kmax), maximum tangential curvature of the front surface (tKmax), minimum pachymetry (Pachymin), maximum elevation of the front surface (ELEF), and maximum elevation of the back surface (ELEB). Distances between the locations were calculated and tested for a correlation with keratoconus severity and distance between cone and corneal vertex.

Results

Cone locations derived from the curvature maps (Kmax, tKmax) showed the lowest agreement with the locations determined by pachymetry or elevation maps. The largest distances were found between Kmax and Pachymin [Median and Interquartile range: 1.19 mm (0.87, 1.60)], Kmax and ELEB [1.12 mm (0.79, 1.41)], and Kmax and ELEF [0.97 mm (0.64, 1.27)]. Low distances (<0.5 mm) were calculated between ELEB and ELEF, and ELEB and Pachymin. All of the calculated distances between the locations showed a significant negative correlation with keratoconus severity and most of them increased significantly with a more peripheral position of the cone (p < 0.05).

Conclusions

There was low consistency between different methods for describing the location of a keratoconus. Curvature-based determinations of the cone center (Kmax, tKmax) showed the highest deviations and should not be used for that purpose. However, the discrepancies between different cone location methods diminished with increasing disease severity and more central position of the cone.
Functional analysis of the bZIP-type transcription factors AtfA and AtfB in Aspergillus nidulans
Authors: Kocsis Beatrix , Lee Mi-Kyung , Yu Jae-Hyuk , Nagy Tibor , Daróczi Lajos , Batta Gyula , Pócsi István , Leiter Éva
Published: September 2022

Transcription factors (TFs) with the basic leucin zipper domain are key elements of the stress response pathways in filamentous fungi. In this study, we functionally characterized the two bZIP type TFs AtfA and AtfB by deletion (Δ) and overexpression (OE) of their encoding genes in all combination: ΔatfA, ΔatfB, ΔatfAΔatfB, ΔatfAatfBOE, ΔatfBatfAOE, atfAOE, atfBOE and atfAOEatfBOE in Aspergillus nidulans. Based on our previous studies, ΔatfA increased the sensitivity of the fungus to oxidative stress mediated by menadione sodium bisulfite (MSB) and tert-butylhydroperoxide (tBOOH), while ΔatfB was not sensitive to any oxidative stress generating agents, namely MSB, tBOOH and diamide at all. Contrarily, the ΔatfB mutant was sensitive to NaCl, but tolerant to sorbitol. Overexpression of atfB was able to compensate the MSB sensitivity of the ΔatfA mutant. Heavy metal stress elicited by CdCl2 reduced diameter of the atfBOE and atfAOEatfBOE mutant colonies to about 50% of control colony, while the cell wall stress generating agent CongoRed increased the tolerance of the ΔatfA mutant. When we tested the heat stress sensitivity of the asexual spores (conidiospores) of the mutants, we found that conidiospores of ΔatfAatfBOE and ΔatfBatfAOE showed nearly 100% tolerance to heat stress. Asexual development was negatively affected by ΔatfA, while atfAOE and atfAOE coupled with ΔatfB increased the number of conidiospores of the fungus approximately 150% compared to the control. Overexpression of atfB led to a 25% reduction in the number of conidiospores, but increased levels of abaA mRNA and size of conidiospores. Sexual fruiting body (cleistothecium) formation was diminished in the ΔatfA and the ΔatfAΔatfB mutants, while relatively elevated in the ΔatfB and the ΔatfBatfAOE mutants. Production of the mycotoxin sterigmatocystin (ST) was decreased to undetectable levels in the ΔatfA mutant, yet ST production was restored in the ΔatfAΔatfB mutant, suggesting that ΔatfB can suppress ST production defect caused by ΔatfA. Levels of ST were also significantly decreased in the ΔatfAatfBOE, ΔatfBatfAOE and atfAOEatfBOE mutants.
Effects of tillage and biochar on soil physiochemical and microbial properties and its linkage with crop yield
Authors: Chen Wenju , Li Peipei , Li Fang , Xi Jingjing , Han Yanlai
Published: September 2022

Vertisols are clayey soils with a high potential for improving production. Therefore, understanding the impact of tillage and fertilization on soil physicochemical properties and microbial community is essential for improving the vertisols with a high montmorillonite and smectite clay content. A 3-year field experiment was conducted to compare the effects of different tillage and fertilization practices at three depths of the vertisol under the wheat–maize cropping system in the North China Plain. The experimental treatments included rotary tillage without fertilization (R-CK), rotary tillage with chemical nitrogen (N), phosphorus (P), and potassium (K) fertilization (R-NPK), R-NPK plus biochar (R-NPKB), deep tillage without fertilization (D-CK), deep tillage with chemical N, P, and K fertilization (D-NPK), and D-NPK plus biochar (D-NPKB). The results showed that D-NPKB significantly improved winter wheat and summer maize yields by 14.4 and 3.8%, respectively, compared with R-NPK. The nitrate (NO3––N) content of the deeper soil layer in D-NPKB was significantly higher than that in D-NPK. Meanwhile, biochar application increased the pH in the three layers. Compared with R-NPK, D-NPKB significantly increased the average content of available phosphorus (AP), soil organic carbon (SOC), and total nitrogen (TN) by 73.7, 18.5, and 19.0%, respectively. Meanwhile, Gaiellale, Sphingomonadaceae, and Nocardioidaceae were the predominant bacteria at the family level across all treatments, with a total relative proportion ranging from 14.1 to 23.6%. In addition, the abundance of Bacillaceae in deep tillage was 9.4% higher in the 20–30-cm soil layer than that in rotary tillage. Furthermore, the correlation analysis revealed a significant positive correlation between crop yield and chemical factors such as NO3––N and the abundances of Gaiellalea, Sphingomonadaceae, and Nocardioidaceae. The findings collectively indicated that deep tillage combined with biochar application could increase the soil nutrients and modify the bacterial structure in the deeper soil layer and therefore will be beneficial for improving the productivity of the vertisols.