bluuebunny/crossref_metadata_2025 huggingface dataset:
The ABC toxin complex from Yersinia entomophaga can package three different cytotoxic components expressed from distinct genetic loci in an unfolded state: the structures of both shell and cargo
Authors: Busby Jason N. , Trevelyan Sarah , Pegg Cassandra L. , Kerr Edward D. , Schulz Benjamin L. , Chassagnon Irene , Landsberg Michael J. , Weston Mitchell K. , Hurst Mark R. H. , Lott J. Shaun
Published: March 2024

Bacterial ABC toxin complexes (Tcs) comprise three core proteins: TcA, TcB and TcC. The TcA protein forms a pentameric assembly that attaches to the surface of target cells and penetrates the cell membrane. The TcB and TcC proteins assemble as a heterodimeric TcB–TcC subcomplex that makes a hollow shell. This TcB–TcC subcomplex self-cleaves and encapsulates within the shell a cytotoxic `cargo' encoded by the C-terminal region of the TcC protein. Here, we describe the structure of a previously uncharacterized TcC protein from Yersinia entomophaga, encoded by a gene at a distant genomic location from the genes encoding the rest of the toxin complex, in complex with the TcB protein. When encapsulated within the TcB–TcC shell, the C-terminal toxin adopts an unfolded and disordered state, with limited areas of local order stabilized by the chaperone-like inner surface of the shell. We also determined the structure of the toxin cargo alone and show that when not encapsulated within the shell, it adopts an ADP-ribosyltransferase fold most similar to the catalytic domain of the SpvB toxin from Salmonella typhimurium. Our structural analysis points to a likely mechanism whereby the toxin acts directly on actin, modifying it in a way that prevents normal polymerization.
Effect of a halogen bond on the crystal structure of terphenyldicarboxylic acid derivatives
Authors: Osipov Artyom A. , Nayfert Sergey A. , Sobalev Sergey A. , Rajakumar Kanthapazham , Zherebtsov Dmitry A. , Spiridonova Dar'ya V. , Vinnik Denis A.
Published: March 2024

The syntheses and structures of dimethyl [11,21:24,31-terphenyl]-14,34-dicarboxylate (1), dimethyl 22,25-diiodo[11,21:24,31-terphenyl]-14,34-dicarboxylate (2), potassium [11,21:24,31-terphenyl]-14,34-dicarboxylate (3) and dimethyl [1,1′-biphenyl]-4,4′-dicarboxylate (4) are reported. Neighboring phenyl rings in compounds 1, 3 and 4 have a planar structure (torsion angles are 0.6–4.1°) and the molecules are packed into regular layers. In the structure of the iodinated derivative of terphenyldicarboxylic acid (2), the middle benzene ring of the terphenyl fragment is rotated relative to the other rings by 64° due to the repulsion between the protons and the iodine atoms of neighboring rings. The formation of halogen bonds between iodine and oxygen atoms of the carbonyl group leads to the movement of molecules of one layer into another layer and the loss of layered structure. Potassium [11,21:24,31-terphenyl]-14,34-dicarboxylate (3) forms crystals with an ionic structure. The coordination number of the potassium cation is eight and the resulting coordination polyhedron is a distorted square antiprism. Dianions in the potassium salt crystal are packed in layers similar to the layers in the dimethyl ethers 1 and 4. Salt 3 has high thermal stability to 500°C.
Attractive and repulsive forces in a crystal of [Rb(18-crown-6)][SbCl6] under high pressure
Authors: Rusanov Eduard B. , Wörle Michael D. , Kovalenko Maksym V. , Domasevitch Kostiantyn V. , Rusanova Julia A.
Published: March 2024

The compression behavior of [Rb(18-crown-6)][SbCl6] crystal under pressure up to 2.16 (3) GPa was investigated in a diamond anvil cell (DAC) using a mixture of pentane–isopentane (1:4) as the pressure-transmitting fluid. The compound crystallizes in trigonal space group R
3 and no phase transition was observed in the indicated pressure range. The low value of pressure bulk modulus [9.1 (5) GPa] found in this crystal is a characteristic of soft materials with predominant dispersive and electrostatic interaction forces. The nonlinear relationship between unit-cell parameters under high pressure is attributed to the influence of reduced intermolecular H...Cl contacts under pressure over 0.73 GPa. It also explains the high compression efficiency of [Rb(18-crown-6)][SbCl6] crystals at relatively low pressures, resulting in a significant shift of the Rb atom to the center of the crown ether cavity. At pressures above 0.9 GPa, steric repulsion forces begin to play a remarkable role, since an increasing number of interatomic H...Cl and H...H contacts become shorter than the sum of their van der Waals (vdW) radii. Below 0.9 GPa, both unit-cell parameter dependences (P–a and P–c) exhibit hysteresis upon pressure release, demonstrating their influence on the disordered model of Rb atoms. The void reduction under pressure also demonstrates two linear sections with the inflection point at 0.9 GPa. Compression of the crystal is accompanied by a significant decrease in the volume of the voids, leading to the rapid approach of Rb atoms to the center of the crown ether cavity. For the Rb atom to penetrate into the center of the crown ether cavity in [Rb(18-crown-6)][SbCl6], it is necessary to apply a pressure of about 2.5 GPa to disrupt the balance of atomic forces in the crystal. This sample serves as a compression model demonstrating the influence of both attractive and repulsive forces on the change in unit-cell parameters under pressure.
Growth, crystal structure and IR luminescence of KSrY1–x
Er
x
(BO3)2
Authors: Kuznetsov Artem B. , Kokh Konstantin A. , Gorelova Liudmila A. , Sofich Dmitry O. , Sagatov Nursultan , Gavryushkin Pavel N. , Vereshchagin Oleg S. , Bocharov Vladimir N. , Shevchenko Vyacheslav S. , Kokh Alexander E.
Published: March 2024

A series of novel KSrY1–x
Er
x
(BO3)2 (x = 0–1) phosphors that emit near-infrared radiation was synthesized using solid-state methods. Pure Y and Er crystals were grown using a KF flux via the top-seeded solution growth technique. In situ high-temperature single crystal X-ray diffraction, Raman spectroscopy and DFT calculations were used for characterization. Within the series, a polymorphic phase transition from space group P21/m to R
3
m was discovered between 550 and 600°C. The concentration dependence of the luminescence intensity was measured for the samples. A strong emission of Er3+ electron transition 4I13/2 → 4I15/2 was detected within the 1529–1549 nm range, with the maximum observed for the KSrY0.4Er0.6(BO3)2 composition.
Driving forces behind the adoption of IEQ principles in building design: evidence from Nigerian quantity surveying firms
Authors: Aliu John , Agbaje Doyin Hellen , Oke Ayodeji Emmanuel , Ebekozien Andrew
Published: March 2024

Purpose

The main objective of this study is to evaluate the driving forces behind the adoption of indoor environmental quality (IEQ) principles in building designs from the perspectives of Nigerian quantity surveying firms.

Design/methodology/approach

A quantitative approach was used which involved administering a well-structured questionnaire to a sample of 114 quantity surveyors. The collected data were analyzed using various statistical methods, including frequencies, percentages, mean item scores, Kruskal–Wallis test and exploratory factor analysis.

Findings

The top five ranked drivers were climate change mitigation, conservation of natural resources, reduction of waste and pollution, use of sustainable building materials and development of new materials and building systems. Based on the factor analysis, the study identified five clusters of drivers: (1) health and well-being drivers (2) economic drivers (3) environmental drivers (4) innovation and technology drivers and (5) regulatory drivers.

Practical implications

The findings from this study suggest that to effectively integrate IEQ principles, quantity surveying firms should consider developing comprehensive guidelines and checklists that align with the identified drivers and clustered categories. These resources can serve as practical tools for project teams, facilitating a structured and holistic approach to the incorporation of IEQ factors throughout the project lifecycle.

Originality/value

The study’s identification of the top drivers and the subsequent clustering of these drivers into five distinct categories contributes to the existing body of knowledge on IEQ. This approach provides a structured framework for comprehensively understanding the factors influencing IEQ adoption, offering a valuable tool for researchers, policymakers and industry practitioners.
An observational study of teachers' and students' behaviors in synchronous online classrooms
Authors: González Carlos , Ponce Daniel
Published: March 2024

Purpose

This paper aims first to describe the most prevalent teachers’ and students’ behaviors in synchronous online classes in emergency remote teaching; second, to discern behavior profiles and third, to investigate what features explain the observed behaviors.

Design/methodology/approach

An adapted COPUS observation protocol was employed to observe 292 online classes from 146 higher education teachers.

Findings

The most prevalent behaviors were: Presenting for teachers and Receiving for students, followed by Teachers Guiding and Students Talking to Class. Furthermore, cluster analysis showed two groups: Traditional and Interactive. The variables that better explained belonging to the Interactive lecture group were disciplinary area – social sciences and humanities –and teaching in technical institutions.

Practical implications

In a context where higher education institutions intend to project the lessons learned into post-pandemic learning experiences, this study provides observational evidence to realize the full potential expected from online and blended teaching and learning.

Originality/value

Despite the prevalence of synchronous online lectures during COVID-19, there is a paucity of observational studies on the actual behaviors that occurred in this context. Most research has been based on surveys and interviews. This study addresses this gap.
Leading the sustainable dimension of business – the responsible leadership way
Authors: Janaswamy Kiranmai , Sarkar Shulagna , Mishra Ram Kumar , Das Gupta Ananda
Published: March 2024

Purpose

The word “sustainability” is discussed in several ways since MDGs and SDGs have gained business attention. Sustainability is not just about something’s durability; it also looks at how to advance development projects without endangering the development of others nearby, both now and in the future (Fink, 2000). It is a drive that weaves together the different facets, including the social, economic, and environmental. World Economic Forum’s has emphasised the need for responsive and responsible leadership. It is in this context, the paper focuses on studying the transformational shift in the role and responsibility of leaders in demonstrating responsible leadership and discusses the ‘Locust and Honeybee Leadership styles’.

Design/methodology/approach

Literature portrays that responsible leaders are recognized by their qualities, competency and skills. One such model is “Locust and Honeybee Leadership,” where the two leadership styles are contrasted. The paper attempts to discuss the varied contexts of these leadership styles and attempts to draw a matrix on the commonalities of both set in an Indian context. A leader should always have openness of thought and integrity.

Findings

Value systems are important for a leader. The major challenge is developing globally responsible leaders and managers who can perform effectively under these conditions of enormous challenge, as they are chronically pushed beyond their capabilities into unexpected roles and unfamiliar domains. Situations compel leaders to make decisions, yet the styles determine the way the decisions are taken. The paper is unique as it discusses the commonalities and contrasting perspectives of the “Locust and Honeybee Leadership.”

Research limitations/implications

The paper is limited to a small number of respondents and is set in Indian context.

Originality/value

To the best of the authors’ knowledge, the paper is an original piece of work and expresses thoughts of the authors on establishing a sustainable leadership model.
Enabling successful change in a high-demand working environment: a case study in a health care organization
Authors: Clarke Emma , Näswall Katharina , Wong Jennifer , Pawsey Fleur , Malinen Sanna
Published: March 2024

Purpose

The anticipation of organizational change and the transition process often creates uncertainty for employees and can lead to stress and anxiety. It is therefore essential for all organizations, especially those that operate in high-demand working environments, to support the well-being of staff throughout the change process.

Design/methodology/approach

Research on how employees respond to the organizational change of relocating to a new work space is limited. To fill this gap in the research, we present a case study examining the well-being of clinical and health care employees before and after a disruptive change: relocation in workplace facilities. In addition, factors that enabled successful change in this high-stress, high-demand working environment were investigated. Interviews were conducted with 20 participants before the relocation and 11 participants after relocation. Following an inductive approach, data were analyzed using thematic analysis to identify key themes.

Findings

Our findings suggest that a supportive team, inclusive leadership and a psychologically safe environment, may buffer negative employee well-being outcomes during disruptive organizational change.

Originality/value

This research contributes to the literature on successful organizational change in health care by highlighting the resources which support well-being throughout the change process and enabling the successful transition to a new facility.
Downside risk in Dow Jones equity markets: hedging and portfolio management during COVID-19 pandemic and the Russia–Ukraine war
Authors: Said Amira , Ouerfelli Chokri
Published: March 2024

Purpose

This paper aims to examine the dynamic conditional correlation (DCC) and hedging ratios between Dow Jones markets and oil, gold and bitcoin. Using daily data, including the COVID-19 pandemic and the Russia–Ukraine war. We employ the DCC-generalized autoregressive conditional heteroskedasticity (GARCH) and asymmetric DCC (ADCC)-GARCH models.

Design/methodology/approach

DCC-GARCH and ADCC-GARCH models.

Findings

The most of DCCs among market pairs are positive during COVID-19 period, implying the existence of volatility spillovers (Contagion-effects). This implies the lack of additional economic gains of diversification. So, COVID-19 represents a systematic risk that resists diversification. However, during the Russia–Ukraine war the DCCs are negative for most pairs that include Oil and Gold, implying investors may benefit from portfolio-diversification. Our hedging analysis carries significant implications for investors seeking higher returns while hedging their Dow Jones portfolios: keeping their portfolios unhedged is better than hedging them. This is because Islamic stocks have the ability to mitigate risks.

Originality/value

Our paper may make a valuable contribution to the existing literature by examining the hedging of financial assets, including both conventional and Islamic assets, during periods of stability and crisis, such as the COVID-19 pandemic and the Russia–Ukraine war.
The impact of marketing appeals on consumer willingness to participate in virtual CSR co-creation
Authors: Zhang Jiemei , Tang Bingxin , Lyu Bei , Song Zhaoran
Published: March 2024

Purpose

This study explores how businesses can effectively market functional and emotional benefits through virtual corporate social responsibility co-creation (VCSRC) initiatives. The aim is to enhance customer engagement through these initiatives.

Design/methodology/approach

The initial phase of this study involved recruiting 185 Chinese university students as participants. These individuals were randomly distributed into four distinct experimental groups, each designed to investigate the influence of varied marketing appeals and common in-group identity (CIGI) on consumer engagement willingness. This phase also sought to elucidate the mediating role of CSR associations in these dynamics. Following this, the second study engaged 570 individuals, recruited through “Credamo,” for a group-based experiment. This subsequent phase was intended to validate the findings of the preliminary study and explore the varying intensities of interaction between different marketing appeals and CIGI, with a particular focus on the dichotomy of independent and interdependent self-construals.

Findings

The study delineates a detailed relationship between consumers' willingness to participate in VCSRC, marketing appeals, and common in-group identity, revealing that strong common in-group identity correlates with a preference for functional appeals, while a weaker in-group identity inclines towards emotional appeals. Notably, interdependent self-construal significantly influences consumer responses, intensifying the interaction between in-group identity and marketing appeal and thereby influencing participation willingness. Moreover, CSR associations emerge as key mediators in this interaction, underscoring their role in enhancing the effectiveness of VCSRC strategies. These insights provide a new understanding of the crucial impact of consumer identity traits on marketing strategy efficacy.

Originality/value

This research stands as a trailblazing endeavor in evaluating the effects of varied advertising appeals under the VCSR paradigm. It probes into the foundational mechanisms, leveraging insights from interaction alignment theory and persuasion theory to elucidate the processes involved.
Formation and hazard of ethyl carbamate and construction of genetically engineered Saccharomyces cerevisiae strains in Huangjiu (Chinese grain wine)
Authors: Li Mi , Jia Wei
Published: March 2024

Huangjiu, a well‐known conventional fermented Chinese grain wine, is widely consumed in Asia for its distinct flavor. Trace amounts of ethyl carbamate (EC) may be generated during the fermentation or storage process. The International Agency for Research on Cancer elevated EC to a Class 2A carcinogen, so it is necessary to regulate EC content in Huangjiu. The risk of intake of dietary EC is mainly assessed through the margin of exposure (MOE) recommended by the European Food Safety Authority, with a smaller MOE indicating a higher risk. Interventions are necessary to reduce EC formation. As urea, one of the main precursors of EC formation in Huangjiu, is primarily produced by Saccharomyces cerevisiae through the catabolism of arginine, the construction of dominant engineered fermentation strains is a favorable trend for the future production and application of Huangjiu. This review summarized the formation and carcinogenic mechanism of EC from the perspectives of precursor substances, metabolic pathways after ingestion, and risk assessment. The methods of constructing dominant S. cerevisiae strains in Huangjiu by genetic engineering technology were reviewed, which provided an important theoretical basis for reducing EC content and strengthening practical control of Huangjiu safety, and the future research direction was prospected.
Factors influencing the fate of chemical food safety hazards in the terrestrial circular primary food production system—A comprehensive review
Authors: Huang Weixin , Focker Marlous , van Dongen Katja C. W. , van der Fels ‐ Klerx H. J.
Published: March 2024

Food safety is recognized as a major hurdle in the transition toward circular food production systems due to the potential reintroduction and accumulation of chemical contaminants in these food systems. Effectively managing these hazardous contaminants in a risk‐based manner requires quantitative insights into the factors influencing the presence and fate of contaminants in the entire circular food chain. A systematic literature review was performed to gain an up‐to‐date overview of the known factors and their influence on the transfer and accumulation of contaminants. This review focused on the terrestrial circular primary food production system, including the pathways between waste‐ or byproduct‐based fertilizers, soil, crops, animal feed, and farmed animals. This review revealed an imbalance in research regarding the different pathways: studies on the soil‐to‐crop pathway were most abundant. The factors identified can be categorized as compound‐related (intrinsic) factors, such as hydrophobicity, molecular weight, and chain length, and extrinsic factors, such as soil organic matter and carbon, pH, milk yield of cows, crop age, and biomass. Quantitative data on the influence of the identified factors were limited. Most studies quantified the influence of individual factors, whereas only a few studies quantified the combined effect of multiple factors. By providing a holistic insight into the influential factors and the quantification of their influence on the fate of contaminants, this review contributes to the improvement of food safety management for chemical hazards when transitioning to a circular food system.
Massive continuous irrigation (MCI) and endoscopic debridement as an alternative treatment strategy for refractory abscess‐fistula complexes
Authors: Zhao Yue , Zhang Ji Yu , Ullah Saif , Zheng Qing Fen , Liu Dan , Wang Meng , Zhao Li Xia , Shi Miao , Li De Liang , Liu Bing Rong
Published: March 2024

Objective

To evaluate the feasibility, safety, and efficacy of massive continuous irrigation (MCI) and endoscopic debridement for the treatment of refractory abscess–fistula complexes.

Methods

This was a retrospective single‐center observational study involving 12 patients with refractory abscess–fistula complexes. All patients had experienced long‐term treatment failure or had failed multiple treatment modalities. We used over two catheters and inserted them via the gastrointestinal (GI) tract or percutaneously to form a circulation pathway to achieve MCI of normal saline, endoscopic debridement was then performed. The treatment success rate, irrigation volume and treatment duration, time to abscess–fistula complex closure, intra‐treatment complications, and recurrence rate were recorded.

Results

The treatment success rates were 100%. The median time of previous treatment was 32 days (range 7–912 days). The mean time from the use of the novel treatment strategy to abscess–fistula complex healing was 18.8 ± 11.0 days. The mean volume of irrigation was 10 804 ± 1669 mL/24 h. The mean irrigation time was 16.5 ± 9.2 days, and a median of two irrigation tubes (range 2–5) were used. No complications occurred either during or after the procedure. During the follow‐up of 23.1 ± 18.1 months, no recurrence or adverse events were noted.

Conclusions

MCI and endoscopic debridement may be a feasible, safe, and effective alternative treatment for refractory abscess–fistula complexes. Large prospective studies are needed to validate our results.
Using Drosophila amyloid toxicity models to study Alzheimer's disease
Authors: Tsintzas Elli , Niccoli Teresa
Published: March 2024

Alzheimer's disease (AD) is the most prevalent form of dementia and is characterised by a progressive loss of neurons, which manifests as gradual memory decline, followed by cognitive loss. Despite the significant progress in identifying novel biomarkers and understanding the prodromal pathology and symptomatology, AD remains a significant unmet clinical need. Lecanemab and aducanumab, the only Food and Drug Administration approved drugs to exhibit some disease‐modifying clinical efficacy, target Aβ amyloid, underscoring the importance of this protein in disease aetiology. Nevertheless, in the absence of a definitive cure, the utilisation of preclinical models remains imperative for the identification of novel therapeutic targets and the evaluation of potential therapeutic agents. Drosophila melanogaster is a model system that can be used as a research tool to investigate neurodegeneration and therapeutic interventions. The short lifespan, low price and ease of husbandry/rearing make Drosophila an advantageous model organism from a practical perspective. However, it is the highly conserved genome and similarity of Drosophila and human neurobiology which make flies a powerful tool to investigate neurodegenerative mechanisms. In addition, the ease of transgenic modifications allows for early proof of principle studies for future therapeutic approaches in neurodegenerative research. This mini review will specifically focus on utilising Drosophila as an in vivo model of amyloid toxicity in AD.
“Completely Free”: How a Subsumption of Labour and Nature Framework Explains the Surprising Expressions of “Freedom” by Immigrant Worm pickers in Ontario
Authors: Steckley Joshua
Published: March 2024

Canadian nightcrawler (Lumbricus terrestris) worms, the most popular live bait for recreational freshwater anglers, are hand‐picked from Ontario dairy farmers’ fields by an immigrant labour force working in the middle of the night with limited equipment. Though no picker enjoys the arduous activity of picking worms, they do express feelings of “freedom” and “autonomy” in the job. Such expressions are surprising coming from mostly non‐English‐speaking immigrants, labouring with bent backs under dreary conditions producing profit for their employers in an unregulated industry. To understand these counterintuitive conceptions of freedom, I connect debates around “un/free labour” and “constrained agency” with the Marxist concepts of formal and real subsumption of labour and nature to reveal how the ecological conditions of commodity production shape capitalist control over labour. I argue worm‐picker agency should be understood by seeing how the subsumption of labour is related to the subsumption of nature.
Machine learning‐based nomogram to predict poor response to overnight orthokeratology in Chinese myopic children: A multicentre, retrospective study
Authors: Tang Wenting , Li Jiaqian , Fu Xuelin , Lin Quan , Zhang Li , Luo Xiangning , Zhao Wenjing , Liao Jia , Xu Xinyue , Wang Xiaoqin , Zhang Huidan , Li Jing
Published: March 2024

Purpose

To develop and validate an effective nomogram for predicting poor response to orthokeratology.

Methods

Myopic children (aged 8–15 years) treated with orthokeratology between February 2018 and January 2022 were screened in four hospitals of different tiers (i.e. municipal and provincial) in China. Potential predictors included 32 baseline clinical variables. Nomogram for the outcome (1‐year axial elongation ≥0.20 mm: poor response; <0.20 mm: good response) was computed from a logistic regression model with the least absolute shrinkage and selection operator. The data from the First Affiliated Hospital of Chengdu Medical College were randomly assigned (7:3) to the training and validation cohorts. An external cohort from three independent multicentre was used for the model test. Model performance was assessed by discrimination (the area under curve, AUC), calibration (calibration plots) and utility (decision curve analysis).

Results

Between January 2022 and March 2023, 1183 eligible subjects were screened from the First Affiliated Hospital of Chengdu Medical College, then randomly divided into training (n = 831) and validation (n = 352) cohorts. A total of 405 eligible subjects were screened in the external cohort. Predictors included in the nomogram were baseline age, spherical equivalent, axial length, pupil diameter, surface asymmetry index and parental myopia (p < 0.05). This nomogram demonstrated excellent calibration, clinical net benefit and discrimination, with the AUC of 0.871 (95% CI 0.847–0.894), 0.863 (0.826–0.901) and 0.817 (0.777–0.857) in the training, validation and external cohorts, respectively. An online calculator was generated for free access (http://39.96.75.172:8182/#/nomogram).

Conclusion

The nomogram provides accurate individual prediction of poor response to overnight orthokeratology in Chinese myopic children.
A longitudinal study of societal resilience and its predictors during the Israel‐Gaza war
Authors: Kaim Arielle , Tov Maya Siman , Kimhi Shaul , Marciano Hadas , Eshel Yohanan , Adini Bruria
Published: March 2024

This study assesses the resilience of Israeli society during the ongoing Israel‐Gaza conflict and pinpoints factors that influence this resilience in prolonged national crises. A longitudinal study was carried out with two surveys, both using the same questionnaire to gage societal, community, and individual resilience levels, along with hope, morale, distress, perceived threats, and government support. The initial survey was administered 5 days after the war escalated and the second 1 month later. The study's results reveal a decline in societal resilience over time. The regression analysis identified four major associations at both resilience measurement points. The key variables are community resilience and hope, both contributing positively. Attitudes towards government support (specifically being a government supporter vs. an opponent) also played a role. Additionally, there was a negative association with levels of religiosity, particularly distinguishing between ultra‐orthodox and secular individuals. In the temporal analysis predicting future resilience (from data at the first time point to predict resilience at the second time point), societal resilience at the first measurement was the strongest forecaster of its resilience at the second measurement. Additionally, the main continuous variable from the previous analysis, community resilience, continued to be an influential and positive forecaster in the time‐based analysis. The research suggests that the initial unifying effect of the conflict, similar to a “Rally around the flag” phenomenon, may be short‐lived. The study underlines the importance of community strength, hope, government support, and religious considerations in shaping societal resilience in the face of conflict.
“From All, To All”: Implementing a collaborative online conference to reflect on the daily living of individuals with cerebral palsy
Authors: Vieira Beatriz S. , Airoldi Marina J. , Chalfun Deborah , Bonfim Rafael G. A. S. , Teplicky Rachel , Rosenbaum Peter , Mancini Marisa C. , Brandão Marina B.
Published: March 2024

Background

Translating knowledge to improve paediatric rehabilitation has become a research area of interest. This study describes the development and evaluation of an online conference that brought together perspectives of individuals with cerebral palsy (CP), families, health care professionals, and researchers to discuss the daily living of individuals with CP.

Methods

We anchored the development and implementation of the online conference in the action cycle of the Knowledge to Action Framework. To develop the meeting, we included representatives from each stakeholder group in the programme committee. The conference programme was designed having the lifespan perspective of individuals with CP, from birth to adulthood, as its central core, with themes related to daily living (e.g., self‐care, mobility, and continuing education). Participants' satisfaction with the conference was assessed using an anonymized online survey sent to all participants.

Results

The conference had 1656 attendees, of whom 675 answered the online satisfaction survey. Most participants rated the structure of the conference (i.e., quality of the technical support, audio and video, and online platform) and discussed topics (i.e., relevance, content, discussion, speakers, and available time) positively.

Conclusion

Collaborative conferences that include stakeholders throughout the planning and implementation are a viable, effective knowledge translation strategy that allows for sharing experiences and disseminating knowledge among families and individuals with CP, health care professionals, and researchers.
The role of Afrocultural ethos in African American youth's emotion skill development
Authors: Lozada Fantasy T.
Published: March 2024

Research on African American youth's emotional development provides an incomplete understanding of the cultural influences that shape emotion‐related skills such as emotion expression, regulation, and understanding. In this article, I propose the multiple cultural frameworks of triple quandary theory to characterize the nature of mainstream cultural experiences and minority cultural experiences in current research on emotional development in African American youth. I also discuss Afrocultural ethos as an aspect of African American cultural experiences that shapes African American emotional development, using affect and orality as examples that can be explored and embedded within emotional development research. Finally, I describe important factors for researchers to consider in the study of Afrocultural ethos in affective developmental science.
Accuracy of robotic surgery for dental implant placement: A systematic review and meta‐analysis
Authors: Wu Xin‐Yu , Shi Jun‐Yu , Qiao Shi‐Chong , Tonetti Maurizio S. , Lai Hong‐Chang
Published: March 2024

Objectives

To systematically analyze the accuracy of robotic surgery for dental implant placement.

Materials and Methods

PubMed, Embase, and Cochrane CENTRAL were searched on October 25, 2023. Model studies or clinical studies reporting the accuracy of robotic surgery for dental implant placement among patients with missing or hopeless teeth were included. Risks of bias in clinical studies were assessed. Meta‐analyses were undertaken.

Results

Data from 8 clinical studies reporting on 109 patients and 242 implants and 13 preclinical studies were included. Positional accuracy was measured by comparing the implant plan in presurgery CBCT and the actual implant position in postsurgery CBCT. For clinical studies, the pooled (95% confidence interval) platform deviation, apex deviation, and angular deviation were 0.68 (0.57, 0.79) mm, 0.67 (0.58, 0.75) mm, and 1.69 (1.25, 2.12)°, respectively. There was no statistically significant difference between the accuracy of implants placed in partially or fully edentulous patients. For model studies, the pooled platform deviation, apex deviation, and angular deviation were 0.72 (0.58, 0.86) mm, 0.90 (0.74, 1.06) mm, and 1.46 (1.22, 1.70)°, respectively. No adverse event was reported.

Conclusion

Within the limitation of the present systematic review, robotic surgery for dental implant placement showed suitable implant positional accuracy and had no reported obvious harm. Both robotic systems and clinical studies on robotic surgery for dental implant placement should be further developed.
Intracerebral hemorrhage‐induced brain injury in mice: The role of peroxiredoxin 2‐Toll‐like receptor 4 inflammatory axis
Authors: Du Yang , Wang Jinjin , Zhang Jia , Li Ning , Li Guangshuo , Liu Xinmin , Lin Yijun , Wang Dandan , Kang Kaijiang , Bian Liheng , Zhao Xingquan
Published: March 2024

Background

Peroxiredoxin 2 (Prx2), an intracellular protein that regulates redox reactions, released from red blood cells is involved in inflammatory brain injury after intracerebral hemorrhage (ICH). Toll‐like receptor 4 (TLR4) may be crucial in this process. This study investigated the role of the Prx2‐TLR4 inflammatory axis in brain injury following experimental ICH in mice.

Methods

First, C57BL/6 mice received an intracaudate injection of autologous arterial blood or saline and their brains were harvested on day 1 to measure Prx2 levels. Second, mice received an intracaudate injection of either recombinant mouse Prx2 or saline. Third, the mice were co‐injected with autologous arterial blood and conoidin A, a Prx2 inhibitor, or vehicle. Fourth, the mice received a Prx2 injection and were treated with TAK‐242, a TLR4 antagonist, or saline (intraperitoneally). Behavioral tests, magnetic resonance imaging, western blot, immunohistochemistry/immunofluorescence staining, and RNA sequencing (RNA‐seq) were performed.

Results

Brain Prx2 levels were elevated after autologous arterial blood injection. Intracaudate injection of Prx2 caused brain swelling, microglial activation, neutrophil infiltration, neuronal death, and neurological deficits. Co‐injection of conoidin A attenuated autologous arterial blood‐induced brain injury. TLR4 was expressed on the surface of microglia/macrophages and neutrophils and participated in Prx2‐induced inflammation. TAK‐242 treatment attenuated Prx2‐induced inflammation and neurological deficits.

Conclusions

Prx2 can cause brain injury following ICH through the TLR4 pathway, revealing the Prx2‐TLR4 inflammatory axis as a potential therapeutic target.
Ischemia‐induced cardiac dysfunction is exacerbated in adiponectin‐knockout mice due to impaired autophagy flux
Authors: Sung Hye Kyoung , Tang Jialing , Jahng James Won Suk , Song Erfei , Chan Yee Kwan , Lone Abdul Hadee , Peterson Jeffrey , Abdul‐Sater Ali , Sweeney Gary
Published: March 2024

Strategies to enhance autophagy flux have been suggested to improve outcomes in cardiac ischemic models. We explored the role of adiponectin in mediating cardiac autophagy under ischemic conditions induced by permanent coronary artery ligation. We studied the molecular mechanisms underlying adiponectin's cardio‐protective effects in adiponectin knockout (Ad‐KO) compared with wild‐type (WT) mice subjected to ischemia by coronary artery ligation and H9c2 cardiomyocyte cell line exposed to hypoxia. Systemic infusion of a cathepsin‐B activatable near‐infrared probe as a biomarker for autophagy and detection via noninvasive three‐dimensional fluorescence molecular tomography combined with computerized tomography to quantitate temporal changes, indicated increased activity in the myocardium of WT mice after myocardial infarction which was attenuated in Ad‐KO. Seven days of ischemia increased myocardial adiponectin accumulation and elevated ULK1/AMPK phosphorylation and autophagy assessed by Western blotting for LC3 and p62, an outcome not observed in Ad‐KO mice. Cell death, assessed by TUNEL analysis and the ratio of Bcl‐2:Bax, plus cardiac dysfunction, measured using echocardiography with strain analysis, were exacerbated in Ad‐KO mice. Using cellular models, we observed that adiponectin stimulated autophagy flux in isolated primary adult cardiomyocytes and increased basal and hypoxia‐induced autophagy in H9c2 cells. Real‐time temporal analysis of caspase‐3/7 activation and caspase‐3 Western blot indicated that adiponectin suppressed activation by hypoxia. Hypoxia‐induced mitochondrial reactive oxygen species production and cell death were also attenuated by adiponectin. Importantly, the ability of adiponectin to reduce caspase‐3/7 activation and cell death was not observed in autophagy‐deficient cells generated by CRISPR‐mediated deletion of Atg7. Collectively, our data indicate that adiponectin acts in an autophagy‐dependent manner to attenuate cardiomyocyte caspase‐3/7 activation and cell death in response to hypoxia in vitro and ischemia in mice.
Practical strategies to manage obesity in type 2 diabetes
Authors: Rodbard Helena W. , Barnard‐Kelly Katharine , Pfeiffer Andreas F. H. , Mauersberger Carina , Schnell Oliver , Giorgino Francesco
Published: March 2024

The rising phenomenon of obesity, a major risk factor for the development and progression of type 2 diabetes, is a complex and multifaceted issue that requires a comprehensive and coordinated approach to be prevented and managed. Although novel pharmacological measures to combat obesity have achieved unprecedented efficacy, a healthy lifestyle remains essential for the long‐term success of any therapeutic intervention. However, this requires a high level of intrinsic motivation and continued behavioural changes in the face of multiple metabolic, psychological and environmental factors promoting weight gain, particularly in the context of type 2 diabetes. This review is intended to provide practical recommendations in the context of a holistic, person‐centred approach to weight management, including evidence‐based and expert recommendations addressing supportive communication, shared decision‐making, as well as nutritional and pharmacological therapeutic approaches to achieve sustained weight loss.
Chronic kidney disease and outcomes in hospitalized sickle cell disease patients: A National Inpatient Sample analysis
Authors: Ajibawo Temitope , Okunowo Oluwatimilehin
Published: March 2024

Objectives

To examine the association between chronic kidney disease (CKD) and outcomes in sickle cell disease (SCD) patients.

Methods

Patients ≥18 years with SCD in the National Inpatient Sample database between 2016 and 2018 were identified using ICD‐10‐CM diagnosis codes. A 1:1 propensity‐score matched analysis was performed to compare in‐hospital outcomes of patients with and without CKD.

Results

Of the 366 240 SCD admissions, 19 365 (5.3%) had CKD. The CKD group had higher odds of in‐hospital mortality (odds ratio [OR] 2.59, 95% confidence interval [CI]: 1.63–4.12, p = <.01), blood transfusion (OR 1.67, 95% CI: 1.47–1.90, p < .01), mechanical ventilation (OR 2.20, 95% CI: 1.56–3.12, p = <.01), sepsis (OR 1.75, 95% CI: 1.46–2.10, p < .01), incurred higher costs ($53 255 vs. $47 294, p < .001), but had lower odds of acute chest syndrome (OR 0.71, 95% CI: 0.54–0.95, p = 0.02) and pulmonary embolism (OR 0.45, 95% CI: 0.31–0.67, p < .01).

Conclusions

CKD was associated with higher mortality, higher costs, blood transfusion, sepsis, and mechanical ventilation in SCD patients. Further studies are needed to explore the reasons for the reduced odds of pulmonary embolism and acute chest syndrome.
The plant root economics space in relation to nutrient limitation in Eurasian herbaceous plant communities
Authors: Scheifes Daniil J. P. , te Beest Mariska , Olde Venterink Harry , Jansen André , Kinsbergen Daan T. P. , Wassen Martin J.
Published: March 2024

Plant species occupy distinct niches along a nitrogen‐to‐phosphorus (N:P) gradient, yet there is no general framework for belowground nutrient acquisition traits in relation to N or P limitation. We retrieved several belowground traits from databases, placed them in the “root economics space” framework, and linked these to a dataset of 991 plots in Eurasian herbaceous plant communities, containing plant species composition, aboveground community biomass and tissue N and P concentrations. Our results support that under increasing N:P ratio, belowground nutrient acquisition strategies shift from “fast” to “slow” and from “do‐it‐yourself” to “outsourcing”, with alternative “do‐it‐yourself” to “outsourcing” strategies at both ends of the spectrum. Species' mycorrhizal capacity patterns conflicted with root economics space predictions based on root diameter, suggesting evolutionary development of alternative strategies under P limitation. Further insight into belowground strategies along nutrient stoichiometry is crucial for understanding the high abundance of threatened plant species under P limitation.
Healthcare professionals' experiences of caring for women with false‐positive screening test results in the National Health Service Breast Screening Programme
Authors: Long Hannah A. , Brooks Joanna M. , Maxwell Anthony J. , Peters Sarah , Harvie Michelle , French David P.
Published: March 2024

Background

Understanding healthcare professionals' (HCPs) experiences of caring for women with false‐positive screening test results in the National Health Service Breast Screening Programme (NHSBSP) is important for reducing the impact of such results.

Methods

Interviews were undertaken with 12 HCPs from a single NHSBSP unit, including advanced radiographer practitioners, breast radiographers, breast radiologists, clinical nurse specialists (CNSs), and a radiology healthcare assistant. Data were analysed thematically using Template Analysis.

Results

Two themes were produced: (1) Gauging and navigating women's anxiety during screening assessment was an inevitable and necessary task for all participants. CNSs were perceived as particularly adept at this, while breast radiographers reported a lack of adequate formal training. (2) Controlling the delivery of information to women (including amount, type and timing of information). HCPs reported various communication strategies to facilitate women's information processing and retention during a distressing time.

Conclusions

Women's anxiety could be reduced through dedicated CNS support, but this should not replace support from other HCPs. Breast radiographers may benefit from more training to emotionally support recalled women. While HCPs emphasised taking a patient‐centred communication approach, the use of other strategies (e.g., standardised scripts) and the constraints of the ‘one‐stop shop’ model pose challenges to such an approach.

Patient and Public Contribution

During the study design, two Patient and Public Involvement members (women with false‐positive‐breast screening test results) were consulted to gain an understanding of patient perspectives and experiences of being recalled specifically in the NHSBSP. Their feedback informed the formulations of the research aim, objectives and the direction of the interview guide.
Microbiomes detected by cerebrospinal fluid metagenomic next‐generation sequencing among patients with and without HIV with suspected central nervous system infection
Authors: Zou Shi , Chen Zhong , Tan Yuting , Tan Miao , Guo Wei , Wu Songjie , Liu Jie , Song Shihui , Peng Yongquan , Wang Min , Liang Ke
Published: March 2024

Background

Opportunistic infections in the central nervous system (CNS) can be a serious threat to people living with HIV. Early aetiological diagnosis and targeted treatment are crucial but difficult. Metagenomic next‐generation sequencing (mNGS) has significant advantages over traditional detection methods. However, differences in the cerebrospinal fluid (CSF) microbiome profiles of patients living with and without HIV with suspected CNS infections using mNGS and conventional testing methods have not yet been adequately evaluated.

Methods

We conducted a retrospective cohort study in the first hospital of Changsha between January 2019 and June 2022 to investigate the microbiomes detected using mNGS of the CSF of patients living with and without HIV with suspected CNS infections. The pathogens causing CNS infections were concurrently identified using both mNGS and traditional detection methods. The spectrum of pathogens identified was compared between the two groups.

Results

Overall, 173 patients (140 with and 33 without HIV) with suspected CNS infection were enrolled in our study. In total, 106 (75.7%) patients with and 16 (48.5%) patients without HIV tested positive with mNGS (p = 0.002). Among the enrolled patients, 71 (50.7%) with HIV and five (15.2%) without HIV tested positive for two or more pathogens (p < 0.001). Patients with HIV had significantly higher proportions of fungus (20.7% vs. 3.0%, p = 0.016) and DNA virus (59.3% vs. 21.2%, p < 0.001) than those without HIV. Epstein–Barr virus (33.6%) was the most commonly identified potential pathogen in the CSF of patients living with HIV using mNGS, followed by cytomegalovirus (20.7%) and torque teno virus (13.8%). The top three causative pathogens identified in patients without HIV were Streptococcus (18.2%), Epstein–Barr virus (12.1%), and Mycobacterium tuberculosis (9.1%). In total, 113 patients living with HIV were diagnosed as having CNS infections. The rate of pathogen detection in people living with HIV with a CNS infection was significantly higher with mNGS than with conventional methods (93.8% vs. 15.0%, p < 0.001).

Conclusion

CSF microbiome profiles differ between patients living with and without HIV with suspected CNS infection. mNGS is a powerful tool for the diagnosis of CNS infection among people living with HIV, especially in those with mixed infections.