bluuebunny/crossref_metadata_2025 huggingface dataset:
Syncytia in Fungi
Authors: Mela Alexander P. , Rico-Ramírez Adriana M. , Glass N. Louise
Published: October 2020

Filamentous fungi typically grow as interconnected multinucleate syncytia that can be microscopic to many hectares in size. Mechanistic details and rules that govern the formation and function of these multinucleate syncytia are largely unexplored, including details on syncytial morphology and the regulatory controls of cellular and molecular processes. Recent discoveries have revealed various adaptations that enable fungal syncytia to accomplish coordinated behaviors, including cell growth, nuclear division, secretion, communication, and adaptation of the hyphal network for mixing nuclear and cytoplasmic organelles. In this review, we highlight recent studies using advanced technologies to define rules that govern organizing principles of hyphal and colony differentiation, including various aspects of nuclear and mitochondrial cooperation versus competition. We place these findings into context with previous foundational literature and present still unanswered questions on mechanistic aspects, function, and morphological diversity of fungal syncytia across the fungal kingdom.
Pleiotropic Locus 15q24.1 Reveals a Gender-Specific Association with Neovascular but Not Atrophic Age-Related Macular Degeneration (AMD)
Authors: Kiel Christina , Strunz Tobias ,   International AMD Genomics Consortium (Project Manager Susan Blanton) IAMDGC, Grassmann Felix , Weber Bernhard H. F.
Published: October 2020

Genome-wide association studies (GWAS) have identified an abundance of genetic loci associated with complex traits and diseases. In contrast, in-depth characterization of an individual genetic signal is rarely available. Here, we focus on the genetic variant rs2168518 in 15q24.1 previously associated with age-related macular degeneration (AMD), but only with suggestive evidence. In a two-step procedure, we initially conducted a series of association analyses to further delineate the association of rs2168518 with AMD but also with other complex phenotypes by using large independent datasets from the International AMD Genomics Consortium (IAMDGC) and the UK Biobank. We then performed a functional annotation with reference to gene expression regulation based on data from the Genotype-Tissue Expression (GTEx) project and RegulomeDB. Association analysis revealed a gender-specific association with male AMD patients and an association predominantly with choroidal neovascularization. Further, the AMD association colocalizes with an association signal of several blood pressure-related phenotypes and with the gene expression regulation of CYP1A1, a member of the cytochrome P450 superfamily of monooxygenases. Functional annotation revealed altered transcription factor (TF) binding sites for gender-specific TFs, including SOX9 and SRY. In conclusion, the pleiotropic 15q24.1 association signal suggests a shared mechanism between blood pressure regulation and choroidal neovascularization with a potential involvement of CYP1A1.
Kupffer Cells Sense Free Fatty Acids and Regulate Hepatic Lipid Metabolism in High-Fat Diet and Inflammation
Authors: Diehl Kira L. , Vorac Julia , Hofmann Kristina , Meiser Philippa , Unterweger Iris , Kuerschner Lars , Weighardt Heike , Förster Irmgard , Thiele Christoph
Published: October 2020

A high fat Western-style diet leads to hepatic steatosis that can progress to steatohepatitis and ultimately cirrhosis or liver cancer. The mechanism that leads to the development of steatosis upon nutritional overload is complex and only partially understood. Using click chemistry-based metabolic tracing and microscopy, we study the interaction between Kupffer cells and hepatocytes ex vivo. In the early phase of steatosis, hepatocytes alone do not display significant deviations in fatty acid metabolism. However, in co-cultures or supernatant transfer experiments, we show that tumor necrosis factor (TNF) secretion by Kupffer cells is necessary and sufficient to induce steatosis in hepatocytes, independent of the challenge of hepatocytes with elevated fatty acid levels. We further show that free fatty acid (FFA) or lipopolysaccharide are both able to trigger release of TNF from Kupffer cells. We conclude that Kupffer cells act as the primary sensor for both FFA overload and bacterial lipopolysaccharide, integrate these signals and transmit the information to the hepatocyte via TNF secretion. Hepatocytes react by alteration in lipid metabolism prominently leading to the accumulation of triacylglycerols (TAGs) in lipid droplets, a hallmark of steatosis.
Megadiversity in the Ant Genus Melophorus: The M. rufoniger Heterick, Castalanelli and Shattuck Species Group in the Top End of Australia’s Northern Territory
Authors: Andersen Alan N. , Hoffmann Benjamin D. , Oberprieler Stefanie K.
Published: October 2020

This study contributes to an understanding of megadiversity in the arid-adapted ant genus Melophorus by presenting an integrated genetic, morphological and distributional analysis of diversity within the M. rufoniger group in the 400,000 km2 Top End (northern region) of Australia’s Northern Territory. An earlier study of the Top End’s ant fauna lists eleven species from the M. rufoniger group, but a recent revision of Melophorus described the taxon as a single species occurring throughout most of the Australian mainland. CO1 sequences were obtained for 120 Top End specimens of the M. rufoniger group, along with a specimen from just outside the Top End. We recognize a total of 30 species among the sequenced specimens from the Top End, based on marked CO1 divergence (mean > 9%) in association with morphological differentiation and/or sympatric distribution. The sequenced specimen from just outside the Top End represents an additional species. Our unpublished CO1 data from other specimens from elsewhere in monsoonal Australia indicate that all but two of the 30 sequenced Top End species are endemic to the region, and that such diversity and endemism are similar in both the Kimberley region of far northern Western Australia and in North Queensland. The total number of species in the M. rufoniger group is potentially more than the 93 total species of Melophorus recognized in the recent revision. It has previously been estimated that Melophorus contains at least 1000 species, but our findings suggest that this is a conservative estimate.
The Association of Plasma-Free Branched-Chain Amino Acids with Disease Related Parameters in Ulcerative Colitis
Authors: Papada Efstathia , Amerikanou Charalampia , Gioxari Aristea , Kalogeropoulos Nick , Kaliora Andriana C.
Published: October 2020

Branched-chain amino acids (BCAAs) are involved in immune system’s metabolic pathways and play fundamental role in gut health. Our aim was to assess BCAA plasma levels in patients with ulcerative colitis (UC) and associations of plasma BCAAs with disease-related parameters. This was a case-control study in adult patients with UC and BMI-matched controls. A total of 150 volunteers were screened between May 2016 and June 2017; 43 patients and 34 healthy controls were enrolled. Medical and dietary history (3 × 24 h recalls, MedDiet score), anthropometric measurements, blood and fecal samples were collected. We measured BCAAs in plasma with gas chromatography-mass spectrometry. In patients, fecal calprotectin, lactoferrin, lysozyme and defensin were quantified. Dietary pattern was similar in patients and controls. Plasma-free BCAA profiles did not differ between groups. Regression analysis showed that i) valine was inversely associated with calprotectin (p = 0.007) and ii) isoleucine with age (p = 0.031), after adjusting for age, sex, PMS and smoking. Leucine was negatively associated with age (p = 0.015) after adjusting for age, sex and PMS, but this association vanished when smoking was introduced. No correlation was observed between total BCAAs with any of the parameters. Plasma-free valine is negatively associated with calprotectin in patients with UC.
Regional Economic Impacts from Timber Check Dam Construction—A Comparison with Concrete Check Dam Construction
Authors: Huzita Tomohumi , Noda Ryu , Kayo Chihiro
Published: October 2020

Recently, many mountain disasters caused by natural phenomena, such as typhoons and heavy rains, have struck Japan, where check dams are used as important disaster prevention structures. Meanwhile, increased timber use in Japan is expected to revitalize regional economies, thus drawing attention to the use of timber in check dams. However, comparisons between timber and concrete check dams, in terms of their impact on the regional economy, have been overlooked in previous studies. Therefore, targeting Akita Prefecture in Japan, we evaluated quantitatively the respective regional economic impact of timber and concrete check dam construction through an input–output analysis. An extended input–output table was developed based on the revenue and expenditure data obtained from interviews with check dam construction companies. The construction cost and amount of sediment runoff prevention of a concrete check dam was unified with those of a timber check dam to evaluate their respective economic impact. In both cases, the impact of timber check dam construction was about 12 to 13% larger. In brief, timber check dam construction was found to have a more positive impact on the regional economy than concrete check dam construction. However, with the regional self-sufficiency ratio of the lumber and wood products sector at less than 20%, or the lumber price at 60% of its current price, the economic impact of constructing a timber check dam becomes smaller than that of a concrete check dam. In other words, it is important to harvest, process, and use timber regionally to contribute to the regional economy. Specifically, using timber that is processed, rather than simply harvested, in the region has a larger economic impact on the region. Additionally, in timber check dam construction, procuring inexpensive timber and reducing construction costs may be a trade-off for revitalizing the regional economy.
Numerical Modeling of a Rectangular Hollow-Core Waveguide for the Detection of Fuel Adulteration in Terahertz Region
Authors: Habib Md. Ahasan , Reyes-Vera Erick , Villegas-Aristizabal Juan , Anower Md. Shamim
Published: October 2020

A petrol adulteration sensor based on a rectangular shaped hollow-core photonic crystal fiber is proposed and numerically analyzed in the terahertz regime. The performance of the proposed sensor was evaluated when it is employed to characterize different kerosene mixtures. In this research, the adulterated fuel sample is filled in the rectangular hollow channel and the electromagnetic signal of the terahertz band is also driven through the same channel. The received signal after the interaction of fuel with the terahertz signal will advise the refractive index of the fuel oil inside the core, which will also bear the information of how much extrinsic component is present in the fuel. The finite element method based simulation shows that the proposed sensor can reach a high relative sensitivity of 89% and presents low confinement losses at 2.8 THz. The reported sensing structure is easily realizable with the conventional manufacturing techniques. Consequently, this proposed fiber may be treated as an essential part of real-life applications of petrol adulteration measurements.
Evolution of the Aroma of Treixadura Wines during Bottle Aging
Authors: Vázquez-Pateiro Iván , Arias-González Uxía , Mirás-Avalos José Manuel , Falqué Elena
Published: October 2020

Aroma is a crucial attribute for wine quality, particularly in white wines. Traditionally, the consumption of young white wines is recommended over the year following grape harvest due to potential aroma losses that would worsen wine quality. This study aimed to investigate the evolution of volatile compounds, odor activity value-based aroma notes, and sensory perception in Treixadura (Vitis vinifera L.) dry white wines during a 24-month bottle-aging period. Volatile composition was determined by gas chromatography, and wine sensory evaluation was performed by experts. Wine samples had similar volatile compositions at the time of bottling. The volatile contents of the wines were respectively 322.9, 302.7, 323.0, and 280.9 mg L−1 after 6, 12, 18, and 24 months of bottle storage. Most of the volatiles tended to maintain constant concentrations, or with slight increases in all families of volatiles except for acetates and carbonyl compounds, until two years after harvest (18 months of bottle storage) and, then, concentrations reduced sharply. After 24 months of storage in the bottle, the concentrations of terpenes, C6 compounds, higher alcohols, ethyl esters, fatty acids, acetates, carbonyl compounds, and volatile phenols were reduced by 32%, 47%, 11%, 39%, 50%, 74%, 41%, and 54%, respectively. The 18-month bottle-aged wines showed the highest concentrations of volatiles, as well as the best performance in the sensory evaluation, suggesting that a good balance of the aroma attributes was achieved on this date. In conclusion, the current study suggests that Treixadura wines expressed their maximum aroma potential two years after grape harvest.
Risk Factors for Mortality in Nursing Home Residents: An Observational Study
Authors: García-Gollarte José Fermín , García-Andrade María Montero , Santaeugenia-González Sebastiá J. , Solá Hermida José Carlos , Baixauli-Alacreu Susana , Santabalbina Francisco José Tarazona
Published: October 2020

Purpose: Identifying mortality risk factors in people living in nursing homes could help healthcare professionals to individualize or develop specific plans for predicting future care demands and plan end-of-life care in this population. This study aims to identify mortality risk factors in elderly nursing home (NH) residents, based on variables adapted to this environment, routinely collected and easily accessible to their healthcare professionals. Methods: A prospective, longitudinal, observational study of NH residents aged 65 years and older was carried out collecting sociodemographic, functional and cognitive status, nutritional variables, comorbidities, and other health variables. These variables were analyzed as mortality risk factors by Cox proportional hazard models. Results: A total of 531 residents (75.3% female; average age 86.7 years (SD: 6.6)) were included: 25.6% had total dependence, 53.4% had moderate to severe cognitive impairment, 84.5% were malnourished or at risk of malnutrition, and 79.9% were polymedicated. Risk of mortality (hazard ratio, HR) increased in totally dependent residents (HR = 1.52; p = 0.02) and in those with moderate or severe cognitive impairment ((HR = 1.59; p = 0.031) and (HR = 1.93; p = 0.002), respectively). Male gender (HR = 1.88; p < 0.001), age ≥80 years (HR = 1.73; p = 0.034), hypertension (HR = 1.53; p = 0.012), atrial fibrillation/arrhythmia (HR = 1.43; p = 0.048), and previous record of pneumonia (HR = 1.65; p = 0.029) were also found to be mortality drivers. Conclusion: Age and male gender (due to the higher prevalence of associated comorbidity in these two variables), certain comorbidities (hypertension, atrial fibrillation/arrhythmia, and pneumonia), higher functional and cognitive impairment, and frequency of medical emergency service care increased the risk of mortality in our study. Given their importance and their easy identification by healthcare professionals in nursing homes, these clinical variables should be used for planning care in institutionalized older adults.
“[A]n Exterior Air of Pilgrimage”: The Resilience of Pilgrimage Ecopoetics and Slow Travel from Chaucer’s The Canterbury Tales to Jack Kerouac’s On the Road
Authors: Morrison Susan Signe
Published: October 2020

While the Beats can be seen as critical actors in the environmental humanities, their works should be seen over the longue durée. They are not only an origin, but are also recipients, of an environmentally aware tradition. With Geoffrey Chaucer and Jack Kerouac, we see how a contemporary American icon functions as a text parallel to something generally seen as discrete and past, an instance of the modern embracing, interpreting, and appropriating the medieval. I argue that The Canterbury Tales by Chaucer influenced Kerouac’s shaping of On the Road. In the unpublished autograph manuscript travel diary dating from 1948–1949 (On the Road notebook), Kerouac imagines the novel as a quest tale, thinking of pilgrimage during its gestation. Further, Kerouac explicitly cites Chaucer. His novel can be seen not only in the tradition of Chaucer, but can bring out aspects of pilgrimage ecopoetics in general. These connections include structural elements, the spiritual development of the narrator, reliance on vernacular dialect, acute environmental awareness, and slow travel. Chaucer’s influence on Kerouac highlights how certain elements characteristic of pilgrimage literature persist well into the modern period, in a resilience of form, language, and ecological sensibility.
A Comparative Study of Several Metaheuristic Algorithms to Optimize Monetary Incentive in Ridesharing Systems
Authors: Hsieh Fu-Shiung
Published: October 2020

The strong demand on human mobility leads to excessive numbers of cars and raises the problems of serious traffic congestion, large amounts of greenhouse gas emissions, air pollution and insufficient parking space in cities. Although ridesharing is a potential transport mode to solve the above problems through car-sharing, it is still not widely adopted. Most studies consider non-monetary incentive performance indices such as travel distance and successful matches in ridesharing systems. These performance indices fail to provide a strong incentive for ridesharing. The goal of this paper is to address this issue by proposing a monetary incentive performance indicator to improve the incentives for ridesharing. The objectives are to improve the incentive for ridesharing through a monetary incentive optimization problem formulation, development of a solution methodology and comparison of different solution algorithms. A non-linear integer programming optimization problem is formulated to optimize monetary incentive in ridesharing systems. Several discrete metaheuristic algorithms are developed to cope with computational complexity for solving the above problem. These include several discrete variants of particle swarm optimization algorithms, differential evolution algorithms and the firefly algorithm. The effectiveness of applying the above algorithms to solve the monetary incentive optimization problem is compared based on experimental results.
Usability of IoT and Open Data Repositories for Analyzing Water Pollution. A Case Study in the Czech Republic
Authors: Pavlík Jan , Hrnčírová Markéta , Stočes Michal , Masner Jan , Vaněk Jiří
Published: October 2020

Recently, the process of data opening has intensified, especially thanks to the involvement of many institutions that have not yet shared their data. Some entities provided data to the public long before the trend of open data was pushed to a wider level, but many institutions have only engaged in this process recently thanks to a systemic state-level effort to make data repositories available to the public. Therefore, there are many new potential sources of data available for research, including the area of water management. This article analyses the current state of available data in the Czech Republic—their content, structure, format, availability, costs and other indicators that affect the usability of these data for independent researchers in the area of water management. The case study was conducted to ascertain the levels of accessibility and usability of data in open data repositories and the possibilities of obtaining data from IoT (Internet of Things) devices such as networked sensors where required data is either not available from existing sources, too costly, or otherwise unsuitable for the research. The goal of the underlying research was to assess the impact/ratio of various watershed factors based on monitored indicators of water pollution in a model watershed. Such information would help propose measures for reducing the volume of pollution resulting in increased security in terms of available drinking water for the capital city Prague.
The Inhibition of Serine/Threonine Protein Phosphatase Type 5 Mediates Cantharidin Toxicity to Control Periplaneta americana (L.)
Authors: Sun Hong , Li Yifan , Li Xinyu , Zhang Yalin
Published: October 2020

The American cockroach, Periplaneta americana (L.), is a notorious urban pest. It has developed insecticidal resistance to commonly used insecticides. Cantharidin (CTD) is a defensive toxin derived from blister beetles. It has been verified to have insecticidal toxicity in a range of pests. In this study, we determined the ingestion toxicity of CTD and norcantharidin (NCTD) to P. americana to test whether they had the potential to be effective against P. americana. Bioassays revealed that CTD produces toxicity against P. americana. The median lethal concentration (LC50) value of CTD was 50.92 μg/mL, while NCTD displayed nearly no toxicity against P. americana. The inhibition assays of serine/threonine protein phosphatases (PSPs) in P. americana indicated that CTD and NCTD could inhibit PSPs. The value of the half maximal inhibitory concentration (IC50) of CTD was 7.21 ± 0.94 μM, whereas that of NCTD was higher, at 31.65 ± 3.87 μM. Furthermore, the inhibition effect of CTD on the serine/threonine protein phosphatase type 5 of P. americana (PaPP5) was superior to that of NCTD. Specifically, the IC50 of CTD reached 0.39 ± 0.04 μM, while the IC50 of NCTD was 1.87 ± 0.23 μM. This study paves the way for insect-derived agents (CTD) to be applied toward controlling P. americana and contributes to the development of novel insecticides based on PP5 as a target.
Visual and Topographic Improvement with Epithelium-On, Oxygen-Supplemented, Customized Corneal Cross-Linking for Progressive Keratoconus
Authors: Kamiya Kazutaka , Kanayama Shunsuke , Takahashi Masahide , Shoji Nobuyuki
Published: October 2020

Customized cross-linking has been proposed as an alternative to conventional cross-linking in patients with progressive keratoconus, targeting greater flattening of the cone region and improved visual function. Epithelium-on cross-linking aims to reduce complications associated with epithelial removal, while the addition of oxygen aims to maintain treatment effect. Our study evaluates the combination of these novel treatment strategies to achieve a minimally invasive treatment targeting maximal functional outcomes. This prospective study included 42 eyes undergoing epithelium-on, accelerated, oxygen-supplemented, customized corneal cross-linking for progressive keratoconus. Outcome measures, including refraction, visual acuity, and corneal tomography were obtained at baseline and at 1, 3, and 6 months, and 1 year postoperatively. Logarithm of the minimum angle of resolution (logMAR) uncorrected visual acuity was significantly improved, from 0.87 ± 0.53 preoperatively, to 0.78 ± 0.56 1 year postoperatively (Wilcoxon rank sum test, p = 0.016). LogMAR best spectacle-corrected visual acuity was also significantly improved, from 0.19 ± 0.36 preoperatively, to 0.11 ± 0.33 postoperatively (p = 0.004). Manifest refractive cylinder was significantly decreased, from 4.50 ± 2.96 D preoperatively, to 3.27 ± 2.61 D postoperatively (p = 0.004). The baseline maximum keratometry (Kmax) was 53.04 ± 7.91 D, significantly flattening to 52.25 ± 7.31 D by 1 month, and remaining relatively stable at 1 year postoperatively (52.31 ± 7.50 D) (p < 0.001). No significant adverse events occurred in any eye. Epithelium-on, accelerated, oxygen-supplemented, customized corneal cross-linking is a promising new treatment approach, with reduced maximum keratometry, reduced astigmatism, and improved visual acuity at 1 year, with a favorable safety and patient comfort profile.
Pregnancy Outcomes in Women with Long-Duration Type 1 Diabetes—25 Years of Experience
Authors: Wender-Ozegowska Ewa , Gutaj Paweł , Mantaj Urszula , Kornacki Jakub , Ozegowski Stefan , Zawiejska Agnieszka
Published: October 2020

Aims: Our study aimed to examine the pregnancy outcomes (maternal and fetal) concerning different models of antenatal care across a period of over 25 years (1993–2018) in 459 women with type 1 diabetes. Data from patients with a history of the condition lasting at least 15 years were considered eligible for analysis. Methods: The study group was divided into three cohorts based on the different models of treatment used in Poznan University Hospital, Poland: 1993–2000 (cohort I, n = 91), 2001–2005 (cohort II, n = 83), 2006–2018 (cohort III, n = 284). To identify predictors for the selected dichotomous outcomes, we calculated the risks for fetal or maternal complications as dependent variables for cohorts II and III against cohort I, using multivariate logistic regression analysis. Results: The mean gestational age was 36.8 ± 2.4 weeks in the total cohort. The percentages of deliveries before the 33rd and the 37th weeks was high. We observed a decreasing percentage during the following periods, from 41.5% in the first period to 30.4% in the third group. There was a tendency for newborn weight to show a gradual increase across three time periods (2850, 3189, 3321 g, p < 0.0001). In the last period, we noticed significantly more newborns delivered after 36 weeks with a weight above 4000 g and below 2500 g. Caesarean section was performed in 88% of patients from the whole group, but in the subsequent periods this number visibly decreased (from 97.6%, 86.7%, to 71%, p = 0.001). The number of emergency caesarean sections was lowest in the third period (27.5%, 16.7%, 11.2%, p = 0.006). We observed a decreasing number of “small for gestational age” newborns (SGA) in consecutive periods of treatment (from 24.4% to 8.7%, p = 0.002), but also a higher percentage of “large for gestational age” (LGA) newborns (from 6.1% to 21.6%, p = 0.001). Modification of treatment might be associated with the gradual reduction of SGA rates (cohort I 3.6%, cohort III 2.3% p < 0.0005). Conclusions: Strict glycemic and blood pressure control from the very beginning of pregnancy, as well as modern fetal surveillance techniques, may contribute to the improvement of perinatal outcomes in women with long-duration type 1 diabetes.
Digitized ADOS: Social Interactions beyond the Limits of the Naked Eye
Authors: Bokadia Harshit , Rai Richa , Torres Elizabeth Barbara
Published: October 2020

The complexity and non-linear dynamics of socio-motor phenomena underlying social interactions are often missed by observation methods that attempt to capture, describe, and rate the exchange in real time. Unknowingly to the rater, socio-motor behaviors of a dyad exert mutual influence over each other through subliminal mirroring and shared cohesiveness that escape the naked eye. Implicit in these ratings nonetheless is the assumption that the other participant of the social dyad has an identical nervous system as that of the interlocutor, and that sensory-motor information is processed similarly by both agents’ brains. What happens when this is not the case? We here use the Autism Diagnostic Observation Schedule (ADOS) to formally study social dyadic interactions, at the macro- and micro-level of behaviors, by combining observation with digital data from wearables. We find that integrating subjective and objective data reveals fundamentally new ways to improve standard clinical tools, even to differentiate females from males using the digital version of the test. More generally, this work offers a way to turn a traditional, gold-standard clinical instrument into an objective outcome measure of human social behaviors and treatment effectiveness.
Approaching Gravity as a Continuum Using the Rat Partial Weight-Bearing Model
Authors: Mortreux Marie , Rosa-Caldwell Megan E.
Published: October 2020

For decades, scientists have relied on animals to understand the risks and consequences of space travel. Animals remain key to study the physiological alterations during spaceflight and provide crucial information about microgravity-induced changes. While spaceflights may appear common, they remain costly and, coupled with limited cargo areas, do not allow for large sample sizes onboard. In 1979, a model of hindlimb unloading (HU) was successfully created to mimic microgravity and has been used extensively since its creation. Four decades later, the first model of mouse partial weight-bearing (PWB) was developed, aiming at mimicking partial gravity environments. Return to the Lunar surface for astronauts is now imminent and prompted the need for an animal model closer to human physiology; hence in 2018, our laboratory created a new model of PWB for adult rats. In this review, we will focus on the rat model of PWB, from its conception to the current state of knowledge. Additionally, we will address how this new model, used in conjunction with HU, will help implement new paradigms allowing scientists to anticipate the physiological alterations and needs of astronauts. Finally, we will discuss the outstanding questions and future perspectives in space research and propose potential solutions using the rat PWB model.
Artificial Neural Network as a Tool to Predict Facial Nerve Palsy in Parotid Gland Surgery for Benign Tumors
Authors: Chiesa-Estomba Carlos M , Sistiaga-Suarez Jon A , González-García José Ángel , Larruscain Ekhiñe , Cammaroto Giovanni , Mayo-Yánez Miguel , Lechien Jerome R , Calvo-Henríquez Christian , Altuna Xabier , Medela Alfonso
Published: October 2020

(1) Background: Despite the increasing use of intraoperative facial nerve monitoring during parotid gland surgery or the improvement in the preoperative radiological assessment, facial nerve injury (FNI) continues to be the most feared complication; (2) Methods: patients who underwent parotid gland surgery for benign tumors between June 2010 and June 2019 were included in this study aiming to make a proof of concept about the reliability of an artificial neural networks (AAN) algorithm for prediction of FNI and compared with a multivariate linear regression (MLR); (3) Results: Concerning prediction accuracy and performance, the ANN achieved the highest sensitivity (86.53% vs 46.23%), specificity (95.67% vs 92.59%), PPV (87.28% vs 66.94%), NPV (95.68% vs 83.37%), ROC–AUC (0.960 vs 0.769) and accuracy (93.42 vs 80.42) than MLR; and (4) Conclusions: ANN prediction models can be useful for otolaryngologists—head and neck surgeons—and patients to provide evidence-based predictions about the risk of FNI. As an advantage, the possibility to develop a calculator using clinical, radiological and histological or cytological information can improve our ability to generate patients counselling before surgery.
Transformation-Induced Ductility of Reverse Austenite Evolved by Low-Temperature Tempering of Martensite
Authors: Du Pengju , Chen Peng , Misra Devesh K. , Wu Di , Yi Hongliang
Published: October 2020

A novel steel combining the “quenching and tempering (Q&T)” process was exploited that can achieve the enhancement of austenite by interface migration during tempering the martensitic matrix mixed with films of austenite. A high uniform elongation (12%) combined with high yield tensile strength (1500 MPa) was obtained, which showed distinct advantages over all the other advanced high strength steels under development for a lightweight car body. Furthermore, the effect of austenite on enhancement of ductility in “Q&T” steels with a martensite matrix was elucidated, which suggested that the ductility was promoted by enhancing boundary sliding and delaying work hardening of the martensitic matrix.
Influencing Microstructure of Vanadium Carbide Reinforced FeCrVC Hardfacing during Gas Metal Arc Welding
Authors: Günther Karsten , Bergmann Jean Pierre
Published: October 2020

Vanadium carbide (VC) reinforced FeCrVC hardfacings have become important to improve the lifetime of tools suffering abrasive and impact loads. This is because the microstructural properties of such hardfacings enable the primary VCs to act as obstacles against the penetrating abrasive. Because dilution is supposed to be the key issue influencing the precipitation behaviour of primary carbides during surfacing, the development of deposit welding processes exhibiting a reduced thermal impact, and hence lower dilution to the base material, is the primary focus of the current research. By inserting an additional hot wire in the melt, an approach was developed to separate the material and energy input during gas metal arc welding (GMAW), and hence realised low dilution claddings. The carbide content could be increased, and a grain refinement was observed compared with conventional GMAW. These effects could be attributed to both the reduced dilution and heterogeneous nucleation.
Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents
Authors: B. Gowda Siddabasave Gowda , Gowda Divyavani , Liang Chongsheng , Li Yonghan , Kawakami Kentaro , Fukiya Satoru , Yokota Atsushi , Chiba Hitoshi , Hui Shu-Ping
Published: October 2020

Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are novel endogenous lipids with important physiological functions in mammals. We previously identified a new type of FAHFAs, named short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs), with acetyl or propyl esters of hydroxy fatty acids of carbon chains, C ≥ 20. However, sensitive determination of SFAHFAs is still a challenge, due to their high structural similarity and low abundance in biological samples. This study employs one-step chemical derivatization following total lipid extraction using 2-dimethylaminoethylamine (DMED) for enhanced detection of SFAHFAs. The labeled extracts were subjected to ultrahigh performance liquid chromatography coupled to linear ion trap quadrupole-Orbitrap mass spectrometry (UHPLC/LTQ-Orbitrap MS). Our results demonstrated that the detection sensitivities of SFAHFAs increased after DMED labeling, and is highly helpful in discovering six additional novel SFAHFAs in the cecum and colon contents of WKAH/HKmSlc rats fed with normal and high-fat diet (HFD). The identified DMED labeled SFAHFAs were characterized by their detailed MS/MS analysis, and their plausible fragmentation patterns were proposed. The concentrations of SFAHFAs were significantly reduced in the cecum of HFD group compared to the control. Hence, the proposed method could be a promising tool to apply for the enhanced detection of SFAHFAs in various biological matrices, which in turn facilitate the understanding of their sources, and physiological functions of these novel lipids.
Over-The-Counter Codeine: Can Community Pharmacy Staff Nudge Customers into Its Safe and Appropriate Use?
Authors: Mody Sapana , Kirkdale Charlotte L. , Thornley Tracey , Dickinson Aimi , Avery Anthony J. , Knaggs Roger , Rann Sarah , Bastable Ruth
Published: October 2020

The misuse of opioids, including codeine which is sold over-the-counter (OTC) in United Kingdom (UK) community pharmacies, is a growing public health concern. An educational Patient Safety Card was developed and piloted to see if it nudged customers into the safe and appropriate use of OTC codeine. Exploratory analysis was conducted by (i) recording quantitative interactions for people requesting OTC codeine in community pharmacies; and (ii) a web-based pharmacy staff survey. Twenty-four pharmacies submitted data on 3993 interactions using the Patient Safety Card. Staff found the majority of interactions (91.3%) to be very or quite easy. Following an interaction using the card, customers known to pharmacy staff as frequent purchasers of OTC codeine were more likely not to purchase a pain relief medicine compared to customers not known to staff (5.5% of known customers did not purchase any pain relief product versus 1.1% for unknown customers (χ2 = 41.73, df = 1, p < 0.001)). These results support both the use of a visual educational intervention to encourage appropriate use of OTC codeine in community pharmacy and the principles behind better self-care.
Prosthodontic Applications of Polymethyl Methacrylate (PMMA): An Update
Authors: Zafar Muhammad Sohail
Published: October 2020

A wide range of polymers are commonly used for various applications in prosthodontics. Polymethyl methacrylate (PMMA) is commonly used for prosthetic dental applications, including the fabrication of artificial teeth, denture bases, dentures, obturators, orthodontic retainers, temporary or provisional crowns, and for the repair of dental prostheses. Additional dental applications of PMMA include occlusal splints, printed or milled casts, dies for treatment planning, and the embedding of tooth specimens for research purposes. The unique properties of PMMA, such as its low density, aesthetics, cost-effectiveness, ease of manipulation, and tailorable physical and mechanical properties, make it a suitable and popular biomaterial for these dental applications. To further improve the properties (thermal properties, water sorption, solubility, impact strength, flexural strength) of PMMA, several chemical modifications and mechanical reinforcement techniques using various types of fibers, nanoparticles, and nanotubes have been reported recently. The present article comprehensively reviews various aspects and properties of PMMA biomaterials, mainly for prosthodontic applications. In addition, recent updates and modifications to enhance the physical and mechanical properties of PMMA are also discussed.
Investigation of Surfactant-Polymer Interactions Using Rheology and Surface Tension Measurements
Authors: Yang Jia , Pal Rajinder
Published: October 2020

The interactions between surfactants and a drag-reducing polymer were investigated at a low polymer concentration of 500 ppm, using measurements of the rheology and surface activity of surfactant-polymer solutions. A well-known drag-reducing polymer (anionic sodium carboxymethyl cellulose) and five different surfactants (two anionic, two non-ionic, and one zwitterionic) were selected for the interaction studies. The surfactant-polymer solutions were shear thinning in nature, and they followed the power law model. The interaction between the surfactant and polymer had a strong effect on the consistency index of the solution and a marginal effect on the flow behavior index. The surface tension versus surfactant concentration plots were interpreted in terms of the interactions between surfactant and polymer. The critical aggregation concentration (CAC) of the surfactant was estimated based on the surface tension and rheological data. The CAC values of the same charge surfactants as that of the polymer were found to be significantly higher than other combinations of surfactant and polymer, such as non-ionic surfactant/anionic polymer, and zwitterionic surfactant/anionic polymer.
Controlling Ice Nucleation during Lyophilization: Process Optimization of Vacuum-Induced Surface Freezing
Authors: Allmendinger Andrea , Butt Yuen Li , Mietzner Raphael , Schmidt Felix , Luemkemann Joerg , Lema Martinez Carmen
Published: October 2020

Biopharmaceuticals are often lyophilized to improve their storage stability. Controlling ice nucleation during the freezing step of the lyophilization process is desired to increase homogeneity of product properties across a drug product batch and shorten the primary drying time. The present communication summarizes the process optimization of the freezing process when using vacuum-induced surface freezing to control ice nucleation, in particular for amorphous samples. We characterized freeze-dried samples for solid state properties, and compared these to uncontrolled nucleated samples using bovine serum albumin (BSA) as a model protein. Freezing parameters were optimized to obtain complete nucleation, adequate cake resistance during the subsequent lyophilization cycle, and elegant cakes. We highlight the challenges associated with vacuum-induced surface freezing and propose optimized freezing parameters to control ice nucleation, enabling manufacturing of amorphous samples.
Force Shadows: An Online Method to Estimate and Distribute Vertical Ground Reaction Forces from Kinematic Data
Authors: Weidmann Alexander , Taetz Bertram , Andres Matthias , Laufer Felix , Bleser Gabriele
Published: October 2020

Kinetic models of human motion rely on boundary conditions which are defined by the interaction of the body with its environment. In the simplest case, this interaction is limited to the foot contact with the ground and is given by the so called ground reaction force (GRF). A major challenge in the reconstruction of GRF from kinematic data is the double support phase, referring to the state with multiple ground contacts. In this case, the GRF prediction is not well defined. In this work we present an approach to reconstruct and distribute vertical GRF (vGRF) to each foot separately, using only kinematic data. We propose the biomechanically inspired force shadow method (FSM) to obtain a unique solution for any contact phase, including double support, of an arbitrary motion. We create a kinematic based function, model an anatomical foot shape and mimic the effect of hip muscle activations. We compare our estimations with the measurements of a Zebris pressure plate and obtain correlations of 0.39≤r≤0.94 for double support motions and 0.83≤r≤0.87 for a walking motion. The presented data is based on inertial human motion capture, showing the applicability for scenarios outside the laboratory. The proposed approach has low computational complexity and allows for online vGRF estimation.
A Hybrid Intrusion Detection Model Combining SAE with Kernel Approximation in Internet of Things
Authors: Wu Yukun , Lee Wei William , Gong Xuan , Wang Hui
Published: October 2020

Owing to the constraints of time and space complexity, network intrusion detection systems (NIDSs) based on support vector machines (SVMs) face the “curse of dimensionality” in a large-scale, high-dimensional feature space. This study proposes a joint training model that combines a stacked autoencoder (SAE) with an SVM and the kernel approximation technique. The training model uses the SAE to perform feature dimension reduction, uses random Fourier features to perform kernel approximation, and then random Fourier mapping is explicitly applied to the sub-sample to generate the random feature space, making it possible to apply a linear SVM to uniformly approximate to the Gaussian kernel SVM. Finally, the SAE performs joint training with the efficient linear SVM. We studied the effects of an SAE structure and a random Fourier feature on classification performance, and compared that performance with that of other training models, including some without kernel approximation. At the same time, we compare the accuracy of the proposed model with that of other models, which include basic machine learning models and the state-of-the-art models in other literatures. The experimental results demonstrate that the proposed model outperforms the previously proposed methods in terms of classification performance and also reduces the training time. Our model is feasible and works efficiently on large-scale datasets.
Determining UAV Flight Trajectory for Target Recognition Using EO/IR and SAR
Authors: Stecz Wojciech , Gromada Krzysztof
Published: October 2020

The paper presents the concept of planning the optimal trajectory of fixed-wing unmanned aerial vehicle (UAV) of a short-range tactical class, whose task is to recognize a set of ground objects as a part of a reconnaissance mission. Tasks carried out by such systems are mainly associated with an aerial reconnaissance using Electro-Optical/Infrared (EO/IR) systems and Synthetic Aperture Radars (SARs) to support military operations. Execution of a professional reconnaissance of the indicated objects requires determining the UAV flight trajectory in the close neighborhood of the target, in order to collect as much interesting information as possible. The paper describes the algorithm for determining UAV flight trajectories, which is tasked with identifying the indicated objectives using the sensors specified in the order. The presence of UAV threatening objects is taken into account. The task of determining the UAV flight trajectory for recognition of the target is a component of the planning process of the tactical class UAV mission, which is also presented in the article. The problem of determining the optimal UAV trajectory has been decomposed into several subproblems: determining the reconnaissance flight method in the vicinity of the currently recognized target depending on the sensor used and the required parameters of the recognition product (photo, film, or SAR scan), determining the initial possible flight trajectory that takes into account potential UAV threats, and planning detailed flight trajectory considering the parameters of the air platform based on the maneuver planning algorithm designed for tactical class platforms. UAV route planning algorithms with time constraints imposed on the implementation of individual tasks were used to solve the task of determining UAV flight trajectories. The problem was formulated in the form of a Mixed Integer Linear Problem (MILP) model. For determining the flight path in the neighborhood of the target, the optimal control algorithm was also presented in the form of a MILP model. The determined trajectory is then corrected based on the construction algorithm for determining real UAV flight segments based on Dubin curves.
Growth Performance of Jatropha curcas Cultivated on Local Abandoned Bauxite Mine Soil
Authors: Mingyuan Lim , Samsuri Abd Wahid , Shukor Mohd Yunus , Phang Lai Yee
Published: October 2020

Lack of regulation in bauxite mining has caused land contamination in Malaysia. Land rehabilitation requires plants with excellent adaptability to adverse conditions. Inedible, economical viable crops with environmental co-benefits, like phytoremediation, are preferred. In this study, Jatropha curcas was evaluated for its growth performance in bauxite mine soil. Topsoil and exposed subsoil were sampled from a bauxite mine at Bukit Goh, Kuantan and used for growing J. curcas for 90 days under greenhouse conditions. The soil physicochemical properties, plant growth parameters (increase in number of leaves, plant height, and basal diameter), and oil yield were determined. The findings showed that the mine soils had lower nutrient status than unmined soil as reflected by the total C, N, P, and K values. Al, Fe, and Pb were relatively high in topsoil and subsoil. Plants grown in both mine soils recorded significantly lower increases in plant height and basal diameter but higher increases in number of leaves than that of nursery soil. Oil yield was significantly different between the plants grown in subsoil (46.54%) and topsoil (41.65%). The study demonstrated that J. curcas has the potential to be cultivated as an alternative crop in bauxite mine soil.