bluuebunny/crossref_metadata_2025 huggingface dataset:
Dutch Post-war Fiction Film through a Lens of Psychoanalysis
Authors: Verstraten Peter
Published: May 2021

Dutch Post-war Fiction Film through a Lens of Psychoanalysis is a sequel to Humour and Irony in Dutch Post-war Fiction Film (AUP, 2016), but the two studies can be read separately. Because of the sheer variety of Fons Rademakers's oeuvre, which spans 'art' cinema and cult, genre film and historical epics, each chapter will start with one of his titles to introduce a key concept from psychoanalysis. It is an oft-voiced claim that Dutch cinema strongly adheres to realism, but psychoanalytic theories on desire and fantasy are employed to put this idea into perspective. In the vein of cinephilia, this study brings together canonical titles (ALS TWEE DRUPPELS WATER; SOLDAAT VAN ORANJE) and little gems (MONSIEUR HAWARDEN; KRACHT). It juxtaposes among others GLUCKAUF and DE VLIEGENDE HOLLANDER (on father figures); FLANAGAN and SPOORLOOS (on rabbles and heroes); DE AANSLAG and LEEDVERMAAK (on historical traumas); ANTONIA and BLUEBIRD (on aphanisis)
Citrus peel derived poly-methoxylated flavones (PMF)
Authors: Vajdi Mahdi , Farhangi Mahdieh Abbasalizad
Published: May 2021

Abstract: The prevalence of obesity has increased substantially over the last several decades and several environmental factors have accelerated this trend. Poly-methoxy flavones (PMFs) exist abundantly in the peels of citrus, and their biological activities have been broadly examined in recent years. Several studies have examined the effects of PMFs on obesity and its-related diseases. This systematic review conducted to focus on the effect of PMFs on obesity and its related conditions management. The PubMed, Google Scholar, Scopus, and Science Direct databases were searched for relevant studies published before November 2020. Out of 1,615 records screened, 16 studies met the study criteria. The range of dosage of PMFs was varied from 10 to 200 mg/kg (5–26 weeks) and 1–100 μmol (2h–8 days) across selected animal and in vitro studies, respectively. The literature reviewed shows that PMFs modulate several biological processes associated with obesity such as lipid and glucose metabolism, inflammation, energy balance, and oxidative stress by different mechanisms. All of the animal studies showed significant positive effects of PMFs on obesity by reducing body weight (e.g. reduced weight gain by 21.04%), insulin resistance, energy expenditure, inhibiting lipogenesis and reduced blood lipids (e.g. reduced total cholesterol by 23.10%, TG by 44.35% and LDL by 34.41%). The results of the reviewed in vitro studies have revealed that treatment with PMFs significantly inhibits lipid accumulation in adipocytes (e.g. reduced lipid accumulation by 55–60%) and 3T3-L1 pre-adipocyte differentiation as well by decreasing the expression of PPARγ and C/EBPα and also reduces the number and size of fat cells and reduced TG content in adipocytes by 45.67% and 23.10% and 16.08% for nobiletin, tangeretin and hesperetin, respectively. Although current evidence supports the use of PMFs as a complementary treatment in obesity, future research is needed to validate this promising treatment modality.
Local Earthquake Tomography in the Tjörnes Fracture Zone (North Iceland)
Authors: Abril C. , Tryggvason A. , Gudmundsson Ó. , Steffen R.
Published: May 2021

Local earthquake tomography has been carried out in the Tjörnes Fracture Zone. This transform region connects the Mid‐Atlantic Ridge to the Northern Volcanic Zone in Iceland in a mostly offshore area. The challenge to record seismic information in this area was the motivation for the North ICeland Experiment (NICE). Fourteen ocean‐bottom seismometers and eleven on‐land stations were installed in the project and operated simultaneously with the permanent Icelandic seismic network (SIL) during summer 2004. Data from the experiment were used to estimate P‐ and S‐wave crustal velocities. Also, the gravity anomaly was derived for comparison with the tomographic results. Upper‐crustal velocities are found to be relatively low in the offshore region. In particular, low velocities are mapped along the Húsavík‐Flatey Fault, where a more confined negative gravity anomaly and a sedimentary basin are found. Low velocities are also mapped along the Grímsey Oblique Rift and in a zone connecting these two main lineaments. The northern half of the aseismic Grímsey Shoal appears as a fast anomaly. Furthermore, localized high‐velocity anomalies are found beneath northern Tröllaskagi and Flateyjarskagi Peninsulas, where bedrock dates from Upper and Middle Miocene (10–15 Ma). Regions of low Vp/Vs ratios are mapped at depth along the main lineaments. Low velocities along the lineaments are interpreted as due to fracturing extending into the middle crust, while high velocities in the upper crust beneath Tertiary formations are associated with relic volcanoes. Low Vp/Vs variations along the lineaments are interpreted as due to the presence of supercritical fluids.
Influence of the Distortion of Vertical Wavenumber Spectra on Estimates of Turbulent Dissipation Using the Finescale Parameterization: Observations in the Antarctic Circumpolar Current
Authors: Takahashi Anne , Hibiya Toshiyuki
Published: May 2021

Finescale parameterizations are powerful tools for estimating the global distribution of turbulent kinetic energy dissipation rates ɛ. However, they tend to overestimate ɛ in the Antarctic Circumpolar Current (ACC), where bottom‐reaching mesoscale eddies coexist with energetic internal waves such as wind‐induced near‐inertial waves and bottom‐generated internal lee waves. In this study, we explore the reason for such overestimations by analyzing simultaneous microstructure and finestructure measurements in the ACC. Finescale parameterizations overestimate ɛ where vertical wavenumber spectra of internal wave energy are distorted from the canonical Garrett‐Munk (GM) spectrum by a spectral hump at ∼0.01 cpm wavenumbers. Since shear and strain spectra are integrated over the noise‐free wavenumbers lower than the rolloff wavenumber, the spectral levels are overestimated for such spectra. These distorted shear spectra are mainly located in the upper ocean, while distorted strain spectra near the seafloor. Correlation analyses suggest that shear and strain spectral humps are caused by near‐inertial and lee wave packets superposed on a GM‐like internal wave field, and these wave packets are generated or amplified by geostrophic shear flows.
Restricted Oxygen‐Deficient Basins on the Northern European Epicontinental Shelf Across the Toarcian Carbon Isotope Excursion Interval
Authors: Wang Yunfeng , Ossa Frantz Ossa , Spangenberg Jorge E. , Wille Martin , Schoenberg Ronny
Published: May 2021

The worldwide recognition of the Toarcian carbon isotope excursion (T‐CIE) in organic‐rich sedimentary rocks has been linked to an oceanic anoxic event (OAE) which implies the world's deep oceans were anoxic ∼183 Ma. The majority of independent redox observations used to build this argument were mainly obtained from T‐CIE organic‐rich sediments deposited on northern European epicontinental shelf. However, increasing evidence has shown that this shelf had limited connection with the open ocean, making it unsuitable for reconstructing the T‐CIE ocean redox structure. To unveil such controversy, we present integrated δ15Nbulk and δ15Nker from Dotternhausen profile, Germany, combined with literature data from other T‐CIE profiles. Both δ15Nbulk and δ15Nker values are predominantly between +0.3 and +2.5‰. These positive near‐zero δ15N values imply enhanced N2 fixation by cyanobacteria using molybdenum (Mo)‐based nitrogenase to compensate bioavailable N loss following quantitative denitrification and/or anammox in a strongly redox‐stratified marine setting. Such N isotope composition contradicts the typical sedimentary δ15N values (>3‰) induced by partial water‐column denitrification and/or anammox in modern‐ocean oxygen minimum zones. We rather propose the existence of local oxygen‐deficient basins on northern European epicontinental shelf where dissolved N underwent extensive denitrification and/or anammox causing bioavailable N deficiency. Mo‐based diazotrophy thus played a critical role in discriminating N isotope compositions among multiple hydrographically restricted T‐CIE marginal basins. Restricted oxygen‐depleted environments on the northern European epicontinental shelf unlikely represent the open‐ocean redox landscape. The existence of the global OAE thus needs comprehensive redox investigations on Tethys and/or Panthalassa deep‐sea T‐CIE successions to validate.
Glutamate connectivity associations converge upon the salience network in schizophrenia and healthy controls
Authors: McCutcheon Robert A. , Pillinger Toby , Rogdaki Maria , Bustillo Juan , Howes Oliver D.
Published: May 2021

Alterations in cortical inter-areal functional connectivity, and aberrant glutamatergic signalling are implicated in the pathophysiology of schizophrenia but the relationship between the two is unclear. We used multimodal imaging to identify areas of convergence between the two systems. Two separate cohorts were examined, comprising 195 participants in total. All participants received resting state functional MRI to characterise functional brain networks and proton magnetic resonance spectroscopy (1H-MRS) to measure glutamate concentrations in the frontal cortex. Study A investigated the relationship between frontal cortex glutamate concentrations and network connectivity in individuals with schizophrenia and healthy controls. Study B also used 1H-MRS, and scanned individuals with schizophrenia and healthy controls before and after a challenge with the glutamatergic modulator riluzole, to investigate the relationship between changes in glutamate concentrations and changes in network connectivity. In both studies the network based statistic was used to probe associations between glutamate and connectivity, and glutamate associated networks were then characterised in terms of their overlap with canonical functional networks. Study A involved 76 individuals with schizophrenia and 82 controls, and identified a functional network negatively associated with glutamate concentrations that was concentrated within the salience network (p < 0.05) and did not differ significantly between patients and controls (p > 0.85). Study B involved 19 individuals with schizophrenia and 17 controls and found that increases in glutamate concentrations induced by riluzole were linked to increases in connectivity localised to the salience network (p < 0.05), and the relationship did not differ between patients and controls (p > 0.4). Frontal cortex glutamate concentrations are associated with inter-areal functional connectivity of a network that localises to the salience network. Changes in network connectivity in response to glutamate modulation show an opposite effect compared to the relationship observed at baseline, which may complicate pharmacological attempts to simultaneously correct glutamatergic and connectivity aberrations.
Fatty acid β-oxidation promotes breast cancer stemness and metastasis via the miRNA-328-3p-CPT1A pathway
Authors: Zeng Feng , Yao Mingkang , Wang Yun , Zheng Wei , Liu Shengshan , Hou Zeyu , Cheng Xiaoming , Sun Suhong , Li Taolang , Zhao Hongyuan , Luo Yi , Li Jiang
Published: May 2021

MicroRNAs (miRNA) have been shown to be associated with tumor diagnosis, prognosis, and therapeutic response. MiR-328-3p plays a significant role in breast cancer growth; however, its actual function and how it modulates specific biological functions is poorly understood. Here, miR-328-3p was significantly downregulated in breast cancer, especially in patients with metastasis. Mitochondrial carnitine palmitoyl transferase 1a (CPT1A) is a downstream target gene in the miR-328-3p-regulated pathway. Furthermore, the miR-328-3p/CPT1A/fatty acid β-oxidation/stemness axis was shown responsible for breast cancer metastasis. Collectively, this study revealed that miR-328-3p is a potential therapeutic target for the treatment of breast cancer patients with metastasis, and also a model for the miRNA-fatty acid β-oxidation-stemness axis, which may assist inunderstanding the cancer stem cell signaling functions of miRNA.
RETRACTED ARTICLE: Non-invasive vagus nerve stimulation improves clinical and molecular biomarkers of Parkinson’s disease in patients with freezing of gait
Authors: Mondal Banashree , Choudhury Supriyo , Banerjee Rebecca , Roy Akash , Chatterjee Koustav , Basu Purba , Singh Ravi , Halder Saptak , Shubham Shantanu , Baker Stuart N. , Baker Mark R. , Kumar Hrishikesh
Published: May 2021

Non-invasive vagus nerve stimulation (nVNS) is an established neurostimulation therapy used in the treatment of epilepsy, migraine and cluster headache. In this randomized, double-blind, sham-controlled crossover trial we explored the role of nVNS in the treatment of gait and other motor symptoms in Parkinson’s disease (PD) patients. In a subgroup of patients, we measured selected neurotrophin levels and markers of inflammation and oxidative stress in serum, before and after the experimental intervention. Thirty-three PD patients with associated freezing of gait were randomised to either nVNS or sham. After baseline assessments, patients were instructed to deliver 6 two-minute stimulations (total 12 min/day) of the nVNS/sham device (electroCore, Inc. USA) for one month at home. Patients were then re-assessed. After a washout period of one month, the same patients were allocated to the alternate treatment arm and the same process was followed. Significant improvements in key gait parameters were observed with nVNS, including walking speed, stance time and step length, compared to sham. Similarly, overall motor function (MDS-UPDRS III) also improved significantly following nVNS stimulation. Serum Tumor Necrosis Factor (TNF)-α and glutathione levels decreased and brain-derived neurotrophic factor (BDNF) levels increased significantly (p < 0.05) after treatment with nVNS. Here we present the first double-blind sham-controlled trial evidence of the efficacy and safety of nVNS in the treatment of gait and motor function in patients with PD.
Favourably regulating two-phase flow regime of flow boiling HFE-7100 in microchannels using silicon nanowires
Authors: Alam Tamanna , Li Wenming , Chang Wei , Yang Fanghao , Khan Jamil , Li Chen
Published: May 2021

High performance miniaturized electronic devices require enhanced, compact and reliable thermal management system. As an efficient compact space cooling technique, flow boiling in microchannels has recently gained wide acceptance. However, weak buoyancy effects and microgravity in avionics and numerous space systems operations hinder the performance of flow boiling microchannel thermal management system due to poor bubble departure capacity and unfavorable development of flow regimes. Here we report the flow boiling silicon nanowires (SiNWs) microchannels which can favorably regulate two-phase flow regimes by enhancing explosive boiling, minimizing bubble departure diameter, and smoothing flow regime transition. Extensive experimental investigations along with high speed visualizations are performed. The experiments are performed with the dielectric fluid HFE-7100 in a forced convection loop for wide range of heat and mass fluxes. High speed flow visualizations have been employed at up to 70 k frames per second (fps) to understand the boiling mechanism in terms of bubble dynamics, flow patterns, and flow regime developments for SiNWs microchannels. These studies show that SiNWs reduce intermittent flow regimes (slug/churn), improve rewetting and maintain thin liquid film at wall. Therefore, flow boiling in SiNW microchannels is promising to thermal management owing to its high heat transfer rate with low pressure drop and negligible microgravity sensitivity.
Comparison of minimal residual disease detection in multiple myeloma between the DuraClone and EuroFlow methods
Authors: Yoroidaka Takeshi , Narita Kentaro , Takamatsu Hiroyuki , Fujisawa Momoko , Nakao Shinji , Matsue Kosei
Published: May 2021

In this study, the minimal residual disease (MRD) levels in patients with multiple myeloma (MM) were assessed by comparing the new 8-color single-tube multiparameter flow cytometry method (DuraClone), which reduces the cost of antibodies and labor burden of laboratories, with the EuroFlow next-generation flow (NGF) method. A total of 96 samples derived from 69 patients with MM were assessed to determine the total cell acquisition number (tCAN), percentages of total and normal plasma cells (PCs), and MRD levels using two methods. We found that the tCAN was significantly higher with EuroFlow-NGF than with DuraClone (median 8.6 × 106 vs. 5.7 × 106; p < 0.0001). In addition, a significant correlation in the MRD levels between the two methods was noted (r = 0.92, p < 0.0001). However, in the qualitative analysis, 5.2% (5/96) of the samples showed discrepancies in the MRD levels. In conclusion, the DuraClone is a good option to evaluate MRD in multiple myeloma but it should be used with caution.
DHHC21 deficiency attenuates renal dysfunction during septic injury
Authors: Yang Xiaoyuan , Zheng Ethan , Ma Yonggang , Chatterjee Victor , Villalba Nuria , Breslin Jerome W. , Liu Ruisheng , Wu Mack H. , Yuan Sarah Y.
Published: May 2021

Renal dysfunction is one of the most common complications of septic injury. One critical contributor to septic injury-induced renal dysfunction is renal vascular dysfunction. Protein palmitoylation serves as a novel regulator of vascular function. Here, we examined whether palmitoyl acyltransferase (PAT)-DHHC21 contributes to septic injury-induced renal dysfunction through regulating renal hemodynamics. Multispectral optoacoustic imaging showed that cecal ligation and puncture (CLP)-induced septic injury caused impaired renal excretion, which was improved in DHHC21 functional deficient (Zdhhc21dep/dep) mice. DHHC21 deficiency attenuated CLP-induced renal pathology, characterized by tissue structural damage and circulating injury markers. Importantly, DHHC21 loss-of-function led to better-preserved renal perfusion and oxygen saturation after CLP. The CLP-caused reduction in renal blood flow was also ameliorated in Zdhhc21dep/dep mice. Next, CLP promoted the palmitoylation of vascular α1-adrenergic receptor (α1AR) and the activation of its downstream effector ERK, which were blunted in Zdhhc21dep/dep mice. Vasoreactivity analysis revealed that renal arteries from Zdhhc21dep/dep mice displayed reduced constriction response to α1AR agonist phenylephrine compared to those from wild-type mice. Consistently, inhibiting PATs with 2-bromopalmitate caused a blunted vasoconstriction response to phenylephrine in small arteries isolated from human kidneys. Therefore, DHHC21 contributes to impaired renal perfusion and function during septic injury via promoting α1AR palmitoylation-associated vasoconstriction.
A correlation study of beat-to-beat R-R intervals and pulse arrival time under natural state and cold stimulation
Authors: Peng Rong-Chao , Li Yi , Yan Wen-Rong
Published: May 2021

Beat-to-beat R-R intervals (RRI) and pulse arrival time (PAT) provide pivotal information to evaluate cardiac autonomic functions for predicting arrhythmias and cardiovascular morbidity. However, their relationship has not been clearly understood. In this study, we simultaneously recorded electrocardiograms and photoplethysmograms on 34 subjects in the natural state, and on 55 subjects under the cold stimulation. The RRI and the PAT were calculated and then analyzed using Pearson correlation coefficient. The results showed that the RRI and the PAT were strongly correlated (r = 0.562) and the RRI series were 2.18 ± 0.40 beats advanced to the PAT series. After smoothing, the RRI and the PAT were more correlated in the low frequency than in the high frequency. Furthermore, when involving RRI with the phase effect, the proposed PAT based model showed better performance for blood pressure estimation. We think these results are helpful to understand the underlying regulatory mechanisms of the two cardiovascular factors, and would provide useful suggestions for non-invasive cuffless blood pressure estimation.
A high mutation load of m.14597A>G in MT-ND6 causes Leigh syndrome
Authors: Kishita Yoshihito , Ishikawa Kaori , Nakada Kazuto , Hayashi Jun-Ichi , Fushimi Takuya , Shimura Masaru , Kohda Masakazu , Ohtake Akira , Murayama Kei , Okazaki Yasushi
Published: May 2021

Leigh syndrome (LS) is an early-onset progressive neurodegenerative disorder associated with mitochondrial deficiency. m.14597A>G (p.Ile26Thr) in the MT-ND6 gene was reported to cause Leberʼs hereditary optic neuropathy (LHON) or dementia/dysarthria. In previous reports, less than 90% heteroplasmy was shown to result in adult-onset disease. Here, by whole mitochondrial sequencing, we identified m.14597A>G mutation of a patient with LS. PCR–RFLP analysis on fibroblasts from the patient revealed a high mutation load (> 90% heteroplasmy). We performed functional assays using cybrid cell models generated by fusing mtDNA-less rho0 HeLa cells with enucleated cells from patient fibroblasts carrying the m.14597A>G variant. Cybrid cell lines bearing the m.14597A>G variant exhibited severe effects on mitochondrial complex I activity. Additionally, impairment of cell proliferation, decreased ATP production and reduced oxygen consumption rate were observed in the cybrid cell lines bearing the m.14597A>G variant when the cells were metabolically stressed in medium containing galactose, indicating mitochondrial respiratory chain defects. These results suggest that a high mutation load of m.14597A>G leads to LS via a mitochondrial complex I defect, rather than LHON or dementia/dysarthria.
Emulated retinal image capture (ERICA) to test, train and validate processing of retinal images
Authors: Young Laura K. , Smithson Hannah E.
Published: May 2021

High resolution retinal imaging systems, such as adaptive optics scanning laser ophthalmoscopes (AOSLO), are increasingly being used for clinical research and fundamental studies in neuroscience. These systems offer unprecedented spatial and temporal resolution of retinal structures in vivo. However, a major challenge is the development of robust and automated methods for processing and analysing these images. We present ERICA (Emulated Retinal Image CApture), a simulation tool that generates realistic synthetic images of the human cone mosaic, mimicking images that would be captured by an AOSLO, with specified image quality and with corresponding ground-truth data. The simulation includes a self-organising mosaic of photoreceptors, the eye movements an observer might make during image capture, and data capture through a real system incorporating diffraction, residual optical aberrations and noise. The retinal photoreceptor mosaics generated by ERICA have a similar packing geometry to human retina, as determined by expert labelling of AOSLO images of real eyes. In the current implementation ERICA outputs convincingly realistic en face images of the cone photoreceptor mosaic but extensions to other imaging modalities and structures are also discussed. These images and associated ground-truth data can be used to develop, test and validate image processing and analysis algorithms or to train and validate machine learning approaches. The use of synthetic images has the advantage that neither access to an imaging system, nor to human participants is necessary for development.
Single tryptophan Y160W mutant of homooligomeric E. coli purine nucleoside phosphorylase implies that dimers forming the hexamer are functionally not equivalent
Authors: Narczyk Marta , Mioduszewski Łukasz , Oksiejuk Aleksandra , Winiewska-Szajewska Maria , Wielgus-Kutrowska Beata , Gojdź Adrian , Cieśla Joanna , Bzowska Agnieszka
Published: May 2021

E. coli purine nucleoside phosphorylase is a homohexamer, which structure, in the apo form, can be described as a trimer of dimers. Earlier studies suggested that ligand binding and kinetic properties are well described by two binding constants and two sets of kinetic constants. However, most of the crystal structures of this enzyme complexes with ligands do not hold the three-fold symmetry, but only two-fold symmetry, as one of the three dimers is different (both active sites in the open conformation) from the other two (one active site in the open and one in the closed conformation). Our recent detailed studies conducted over broad ligand concentration range suggest that protein–ligand complex formation in solution actually deviates from the two-binding-site model. To reveal the details of interactions present in the hexameric molecule we have engineered a single tryptophan Y160W mutant, responding with substantial intrinsic fluorescence change upon ligand binding. By observing various physical properties of the protein and its various complexes with substrate and substrate analogues we have shown that indeed three-binding-site model is necessary to properly describe binding of ligands by both the wild type enzyme and the Y160W mutant. Thus we have pointed out that a symmetrical dimer with both active sites in the open conformation is not forced to adopt this conformation by interactions in the crystal, but most probably the dimers forming the hexamer in solution are not equivalent as well. This, in turn, implies that an allosteric cooperation occurs not only within a dimer, but also among all three dimers forming a hexameric molecule.
Effect of TNFα stimulation on expression of kidney risk inflammatory proteins in human umbilical vein endothelial cells cultured in hyperglycemia
Authors: Md Dom Zaipul I. , Pipino Caterina , Krolewski Bozena , O’Neil Kristina , Satake Eiichiro , Krolewski Andrzej S.
Published: May 2021

We recently identified a kidney risk inflammatory signature (KRIS), comprising 6 TNF receptors (including TNFR1 and TNFR2) and 11 inflammatory proteins. Elevated levels of these proteins in circulation were strongly associated with risk of the development of end-stage kidney disease (ESKD) during 10-year follow-up. It has been hypothesized that elevated levels of these proteins in circulation might reflect (be markers of) systemic exposure to TNFα. In this in vitro study, we examined intracellular and extracellular levels of these proteins in human umbilical vein endothelial cells (HUVECs) exposed to TNFα in the presence of hyperglycemia. KRIS proteins as well as 1300 other proteins were measured using the SOMAscan proteomics platform. Four KRIS proteins (including TNFR1) were down-regulated and only 1 protein (IL18R1) was up-regulated in the extracellular fraction of TNFα-stimulated HUVECs. In the intracellular fraction, one KRIS protein was down-regulated (CCL14) and 1 protein was up-regulated (IL18R1). The levels of other KRIS proteins were not affected by exposure to TNFα. HUVECs exposed to a hyperglycemic and inflammatory environment also showed significant up-regulation of a distinct set of 53 proteins (mainly in extracellular fraction). In our previous study, circulating levels of these proteins were not associated with progression to ESKD in diabetes.
Quantitative dual-energy CT for evaluating hepatocellular carcinoma after transarterial chemoembolization
Authors: Yue Xiaofei , Jiang Qiqi , Hu Xuehan , Cen Chunyuan , Song Songlin , Qian Kun , Lu Yuting , Yang Ming , Li Qian , Han Ping
Published: May 2021

We aimed to investigate the role of the quantitative parameters of dual-energy computed tomography (DECT) in evaluating patients with hepatocellular carcinoma (HCC) treated by transarterial chemoembolization (TACE). We retrospectively identified 80 HCC patients (mean age, 56 years; 61 men) treated by TACE who received contrast-enhanced DECT and were retreated by TACE within 7 days between November 2018 and December 2019. Taking digital subtraction angiography (DSA) and CT images as reference standard, two readers measured and calculated the values of normalized iodine concentration at arterial phase (NICAP), normalized iodine concentration at portal venous phase (NICPP), iodine concentration difference (ICD), arterial iodine fraction (AIF) and slope of the spectral Hounsfield unit curve (λHu) by placing matched regions of interests (ROIs) within the tumor active area (TAA), adjacent normal hepatic parenchyma (ANHP) and tumor necrotic area (TNA). Differences between the parameters were analyzed by the Kruskal–Wallis H test. Receiver operating characteristic analysis of the parameters performance in differentiating the three tissues types was performed. AIF exhibited a good performance in distinguishing TAA (0.93 ± 0.31) and ANHP (0.18 ± 0.14), the areas under the receiver operating characteristic curve (AUC) was 0.989, while the λHu exhibited an excellent performance in distinguishing TAA (3.32 ± 1.24) and TNA (0.29 ± 0.27), with an AUC of 1.000. In conclusion, quantitative DECT can be effectively used to evaluate the tumor viability in HCC patients treated by TACE.
A study on improving the current density performances of CO2 electrolysers
Authors: Gu Yueyuan , Wei Jucai , Wu Xu , Liu Xiaoteng
Published: May 2021

Electrochemical CO2 reduction reaction (CO2RR) technology can reduce CO2 emission with converting excess electrical energy to high-value-added chemicals, which however needs further improvement on the electrolyser cell performance. In this work, extensive factors were explored in continuous CO2 electrolysers. Gold, one of the benchmark materials for CO2RR to produce CO, was used as the catalyst. Electrolyser configurations and membrane types have significant influences on cell performance. Compact MEA-constructed gas-phase electrolyser showed better catalytic performance and lower energy consumption. The gas diffusion electrode with a 7:1 mass ratio of total-catalyst-to-polytetrafluoroethylene (PTFE) ionomer exhibited the best performance. At a low total cell voltage of 2.2 V, the partial current density of CO production achieved 196.8 mA cm−2, with 90.6% current efficiency and 60.4% energy efficiency for CO producing respectively. Higher CO selectivity can be achieved using anion exchange membranes, while higher selectivity for hydrogen and formate products can be achieved with cation exchange membranes. This research has pointed out a way on how to improve the CO2RR catalytic performance in flow cells, leaving aside the characteristics of the catalyst itself.
Defining the combined stress response in wild Arachis
Authors: Mota Ana Paula Zotta , Brasileiro Ana Cristina Miranda , Vidigal Bruna , Oliveira Thais Nicolini , da Cunha Quintana Martins Andressa , Saraiva Mario Alfredo de Passos , de Araújo Ana Claudia Guerra , Togawa Roberto C. , Grossi-de-Sá Maria Fatima , Guimaraes Patricia Messenberg
Published: May 2021

Nematodes and drought are major constraints in tropical agriculture and often occur simultaneously. Plant responses to these stresses are complex and require crosstalk between biotic and abiotic signaling pathways. In this study, we explored the transcriptome data of wild Arachis species subjected to drought (A-metaDEG) and the root-knot nematode Meloidogyne arenaria (B-metaDEG) via meta-analysis, to identify core-stress responsive genes to each individual and concurrent stresses in these species. Transcriptome analysis of a nematode/drought bioassay (cross-stress) showed that the set of stress responsive DEGs to concurrent stress is distinct from those resulting from overlapping A- and B-metaDEGs, indicating a specialized and unique response to combined stresses in wild Arachis. Whilst individual biotic and abiotic stresses elicit hormone-responsive genes, most notably in the jasmonic and abscisic acid pathways, combined stresses seem to trigger mainly the ethylene hormone pathway. The overexpression of a cross-stress tolerance candidate gene identified here, an endochitinase-encoding gene (AsECHI) from Arachis stenosperma, reduced up to 30% of M. incognita infection and increased post-drought recovery in Arabidopsis plants submitted to both stresses. The elucidation of the network of cross-stress responsive genes in Arachis contributes to better understanding the complex regulation of biotic and abiotic responses in plants facilitating more adequate crop breeding for combined stress tolerance.
Cause analysis of PM2.5 pollution during the COVID-19 lockdown in Nanning, China
Authors: Mo Zhaoyu , Huang Jiongli , Chen Zhiming , Zhou Bin , Zhu Kaixian , Liu Huilin , Mu Yijun , Zhang Dabiao , Wang Shanshan
Published: May 2021

To analyse the cause of the atmospheric PM2.5 pollution that occurred during the COVID-19 lockdown in Nanning, Guangxi, China, a single particulate aerosol mass spectrometer, aethalometer, and particulate Lidar coupled with monitoring near-surface gaseous pollutants, meteorological conditions, remote fire spot sensing by satellite and backward trajectory models were utilized during 18–24 February 2020. Three haze stages were identified: the pre-pollution period (PPP), pollution accumulation period (PAP) and pollution dissipation period (PDP). The dominant source of PM2.5 in the PPP was biomass burning (BB) (40.4%), followed by secondary inorganic sources (28.1%) and motor vehicle exhaust (11.7%). The PAP was characterized by a large abundance of secondary inorganic sources, which contributed 56.1% of the total PM2.5 concentration, followed by BB (17.4%). The absorption Ångström exponent (2.2) in the PPP was higher than that in the other two periods. Analysis of fire spots monitored by remote satellite sensing indicated that open BB in regions around Nanning City could be one of the main factors. A planetary boundary layer-relative humidity-secondary particle matter-particulate matter positive feedback mechanism was employed to elucidate the atmospheric processes in this study. This study highlights the importance of understanding the role of BB, secondary inorganic sources and meteorology in air pollution formation and calls for policies for emission control strategies.
Specific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of Pseudomonas aeruginosa based on dual molecular recognition
Authors: He Yong , Zhao Hang , Liu Yuanwen , Zhou He
Published: May 2021

The worldwide emergence and spread of antimicrobial resistance is accelerated by irrational administration and use of empiric antibiotics. A key point to the crisis is a lack of rapid diagnostic protocols for antimicrobial susceptibility testing (AST), which is crucial for a timely and rational antibiotic prescription. Here, a recombinant bacteriophage tail fiber protein (TFP) was functionalized on magnetic particles to specifically capture Pseudomonas aeruginosa, while fluorescein isothiocyanate-labeled-magainin II was utilized as the indicator. For solving the magnetic particles’ blocking effects, a reverse assaying protocol based on TFP recognition was developed to investigate the feasibility of detection and AST of P. aeruginosa. P. aeruginosa can be rapidly, sensitively and specifically detected within 1.5 h with a linear range of 1.0 × 102 to 1.0 × 106 colony forming units (CFU)⋅mL−1 and a detection limit of 3.3 × 10 CFU⋅mL−1. Subsequently, AST results, which were consistent with broth dilution results, can be obtained within 3.5 h. Due to the high specificity of the TFP, AST can actually be conducted without the need for bacterial isolation and identification. Based on the proof-of-principle work, the detection and AST of other pathogens can be extended by expressing the TFPs of their bacteriophages.
Multimodal evaluation of hypoxia in brain metastases of lung cancer and interest of hypoxia image-guided radiotherapy
Authors: Corroyer-Dulmont Aurélien , Valable Samuel , Fantin Jade , Chatre Laurent , Toutain Jérôme , Teulier Sylvain , Bazille Céline , Letissier Elise , Levallet Jérôme , Divoux Didier , Ibazizène Méziane , Guillouet Stéphane , Perrio Cécile , Barré Louisa , Serres Sébastien , Sibson Nicola R. , Chapon Françoise , Levallet Guénaëlle , Bernaudin Myriam
Published: May 2021

Lung cancer patients frequently develop brain metastases (BM). Despite aggressive treatment including neurosurgery and external-radiotherapy, overall survival remains poor. There is a pressing need to further characterize factors in the microenvironment of BM that may confer resistance to radiotherapy (RT), such as hypoxia. Here, hypoxia was first evaluated in 28 biopsies from patients with non‑small cell lung cancer (NSCLC) BM, using CA-IX immunostaining. Hypoxia characterization (pimonidazole, CA-IX and HIF-1α) was also performed in different preclinical NSCLC BM models induced either by intracerebral injection of tumor cells (H2030-Br3M, H1915) into the cortex and striatum, or intracardial injection of tumor cells (H2030-Br3M). Additionally, [18F]-FMISO-PET and oxygen-saturation-mapping-MRI (SatO2-MRI) were carried out in the intracerebral BM models to further characterize tumor hypoxia and evaluate the potential of Hypoxia-image-guided-RT (HIGRT). The effect of RT on proliferation of BM ([18F]-FLT-PET), tumor volume and overall survival was determined. We showed that hypoxia is a major yet heterogeneous feature of BM from lung cancer both preclinically and clinically. HIGRT, based on hypoxia heterogeneity observed between cortical and striatal metastases in the intracerebrally induced models, showed significant potential for tumor control and animal survival. These results collectively highlight hypoxia as a hallmark of BM from lung cancer and the value of HIGRT in better controlling tumor growth.
Saline–alkaline stress in growing maize seedlings is alleviated by Trichoderma asperellum through regulation of the soil environment
Authors: Fu Jian , Xiao Yao , Wang Yu-feng , Liu Zhi-hua , Yang Kejun
Published: May 2021

A significant proportion of the land area of Heilongjiang Province, China, is composed of saline–alkaline soil, which severely inhibits maize growth. Although Trichoderma treatment is widely regarded as a promising strategy for improving the soil environment and promoting plant growth, the mechanism through which Trichoderma asperellum enhances maize resistance to saline–alkaline stress is not clear. In this study, we explored the effect of T. asperellum application at different concentrations to soil saline–alkaline environment on the seedlings of two maize cultivars, assessing the biochemical parameters related to oxidation resistance. Increasing spore densities of T. asperellum suspension effectively regulated the soil ion balance in the rhizosphere of maize seedlings, reduced the soil pH by 2.15–5.76% and sodium adsorption ratios by 22.70–54.13%, increased soil nutrient content and enzyme activity, and improved the soil environment for seedling growth. Additionally, T. asperellum treatment increased the maize seedling content of osmo-regulating substances and rate of glutathione:oxidised glutathione (43.86–88.25%) and ascorbate:oxidised ascorbate (25.26–222.32%) by affecting the antioxidant enzyme activity in the roots, increasing reactive oxygen species scavenging, and maintaining the osmotic balance and metabolic homeostasis under saline–alkaline stress. T. asperellum also improved the saline–alkaline tolerance of maize seedlings by improving the root growth characteristics. Moreover, results showed that Trichoderma applied at high concentration had the greatest effect. In conclusion, improvement in the saline–alkaline tolerance of maize seedlings by T. asperellum under saline–alkaline soil conditions may be achieved through diverse effects that vary among maize cultivars.
Potential of therapeutic bile acids in the treatment of neonatal Hyperbilirubinemia
Authors: van der Schoor Lori W. E. , Verkade Henkjan J. , Bertolini Anna , de Wit Sanne , Mennillo Elvira , Rettenmeier Eva , Weber André A. , Havinga Rick , Valášková Petra , Jašprová Jana , Struik Dicky , Bloks Vincent W. , Chen Shujuan , Schreuder Andrea B. , Vítek Libor , Tukey Robert H. , Jonker Johan W.
Published: May 2021

Neonatal hyperbilirubinemia or jaundice is associated with kernicterus, resulting in permanent neurological damage or even death. Conventional phototherapy does not prevent hyperbilirubinemia or eliminate the need for exchange transfusion. Here we investigated the potential of therapeutic bile acids ursodeoxycholic acid (UDCA) and obeticholic acid (OCA, 6-α-ethyl-CDCA), a farnesoid-X-receptor (FXR) agonist, as preventive treatment options for neonatal hyperbilirubinemia using thehUGT1*1humanized mice andUgt1a-deficient Gunn rats. Treatment ofhUGT1*1mice with UDCA or OCA at postnatal days 10–14 effectively decreased bilirubin in plasma (by 82% and 62%) and brain (by 72% and 69%), respectively. Mechanistically, our findings indicate that these effects are mediated through induction of protein levels of hUGT1A1 in the intestine, but not in liver. We further demonstrate that inUgt1a-deficient Gunn rats, UDCA but not OCA significantly decreases plasma bilirubin, indicating that at least some of the hypobilirubinemic effects of UDCA are independent of UGT1A1. Finally, using the synthetic, non-bile acid, FXR-agonist GW4064, we show that some of these effects are mediated through direct or indirect activation of FXR. Together, our study shows that therapeutic bile acids UDCA and OCA effectively reduce both plasma and brain bilirubin, highlighting their potential in the treatment of neonatal hyperbilirubinemia.
Modular network mechanism of CCN1-associated resistance to HSV-1-derived oncolytic immunovirotherapies for glioblastomas
Authors: Monie Dileep D. , Correia Cristina , Zhang Cheng , Ung Choong Yong , Vile Richard G. , Li Hu
Published: May 2021

Glioblastomas (GBMs) are the most common and lethal primary brain malignancy in adults. Oncolytic virus (OV) immunotherapies selectively kill GBM cells in a manner that elicits antitumor immunity. Cellular communication network factor 1 (CCN1), a protein found in most GBM microenvironments, expression predicts resistance to OVs, particularly herpes simplex virus type 1 (HSV-1). This study aims to understand how extracellular CCN1 alters the GBM intracellular state to confer OV resistance. Protein–protein interaction network information flow analyses of LN229 human GBM transcriptomes identified 39 novel nodes and 12 binary edges dominating flow in CCN1high cells versus controls. Virus response programs, notably against HSV-1, and cytokine-mediated signaling pathways are highly enriched. Our results suggest that CCN1high states exploit IDH1 and TP53, and increase dependency on RPL6, HUWE1, and COPS5. To validate, we reproduce our findings in 65 other GBM cell line (CCLE) and 174 clinical GBM patient sample (TCGA) datasets. We conclude through our generalized network modeling and system level analysis that CCN1 signals via several innate immune pathways in GBM to inhibit HSV-1 OVs before transduction. Interventions disrupting this network may overcome immunovirotherapy resistance.
Relative potency of a novel acaricidal compound from Xenorhabdus, a bacterial genus mutualistically associated with entomopathogenic nematodes
Authors: Incedayi Gamze , Cimen Harun , Ulug Derya , Touray Mustapha , Bode Edna , Bode Helge B. , Orenlili Yaylagul Esra , Hazir Selcuk , Cakmak Ibrahim
Published: May 2021

Our study aimed to identify the novel acaricidal compound in Xenorhabdus szentirmaii and X. nematophila using the easyPACId approach (easy Promoter Activated Compound Identification). We determined the (1) effects of cell-free supernatant (CFS) obtained from mutant strains against T. urticae females, (2) CFS of the acaricidal bioactive strain of X. nematophila (pCEP_kan_XNC1_1711) against different biological stages of T. urticae, and females of predatory mites, Phytoseiulus persimilis and Neoseiulus californicus, (3) effects of the extracted acaricidal compound on different biological stages of T. urticae, and (4) cytotoxicity of the active substance. The results showed that xenocoumacin produced by X. nematophila was the bioactive acaricidal compound, whereas the acaricidal compound in X. szentirmaii was not determined. The CFS of X. nematophila (pCEP_kan_XNC1_1711) caused 100, 100, 97.3, and 98.1% mortality on larvae, protonymph, deutonymph and adult female of T. urticae at 7 dpa in petri dish experiments; and significantly reduced T. urticae population in pot experiments. However, the same CFS caused less than 36% mortality on the predatory mites at 7dpa. The mortality rates of extracted acaricidal compound (xenocoumacin) on the larva, protonymph, deutonymph and adult female of T. urticae were 100, 100, 97, 96% at 7 dpa. Cytotoxicity assay showed that IC50 value of xenocoumacin extract was 17.71 μg/ml after 48 h. The data of this study showed that xenocoumacin could potentially be used as bio-acaricide in the control of T. urticae; however, its efficacy in field experiments and its phytotoxicity need to be assessed in future.
Sound pressure level spectrum analysis by combination of 4D PTV and ANFIS method around automotive side-view mirror models
Authors: Kim Dong , Safdari Arman , Kim Kyung Chun
Published: May 2021

This paper proposes a data augmentation method based on artificial intelligence (AI) to obtain sound level spectrum as predicting the spatial and temporal data of time-resolved three-dimensional Particle Tracking Velocimetry (4D PTV) data. A 4D PTV has used to measure flow characteristics of three side mirror models adopting the Shake-The-Box (STB) algorithm with four high-speed cameras on a robotic arm for measuring industrial scale. Helium filled soap bubbles are used as tracers in the wind tunnel experiment to characterize flow structures around automobile side mirror models. Full volumetric velocity fields and evolution of vortex structures are obtained and analyzed. Instantaneous pressure fields are deduced by solving a Poisson equation based on the 4D PTV data. To predict spatial and temporal data of velocity field, artificial intelligence (AI)-based data prediction method has applied. Adaptive Neural Fuzzy Inference System (ANFIS) based machine learning algorithm works well to find 4D missing data behind the automobile side mirror model. Using the ANFIS model, power spectrum of velocity fluctuations and sound level spectrum of pressure fluctuations are successfully obtained to assess flow and noise characteristics of three different side mirror models.
Early pulmonary hypertension is a risk factor for bronchopulmonary dysplasia-associated late pulmonary hypertension in extremely preterm infants
Authors: Kim Hyun Ho , Sung Se In , Yang Mi Sun , Han Yea Seul , Kim Hye Seon , Ahn So Yoon , Jeon Ga Won , Chang Yun Sil , Park Won Soon
Published: May 2021

This study evaluated whether early pulmonary hypertension (PH) in extremely preterm infants (EPIs) at 22–27 weeks of gestation detected clinically with echocardiography at 4–7 postnatal days (PND) is a risk factor for death before 36 weeks post-menstrual age (PMA) or late PH in moderate or severe (m/s) bronchopulmonary dysplasia (BPD) (BPD-PH). We analyzed risk factors for death before 36 weeks PMA or BPD-PH. Among 247 EPIs enrolled, 74 (30.0%) had early PH. Twenty-one (28.4%) infants with early PH and 18 (10.4%) without early PH died before 36 weeks PMA; 14 (18.9%) infants with early PH and 9 (5.2%) without early PH had BPD-PH at 36–38 weeks PMA. Multivariate analysis revealed that early PH (adjusted odds ratio, 6.55; 95% confidence interval, 3.10–13.82, P < 0.05), clinical chorioamnionitis (2.50; 1.18–5.31), intraventricular hemorrhage (grade 3–4) (3.43; 1.26–9.37), and late sepsis (6.76; 3.20–14.28) independently increased the risk of development of death before 36 weeks PMA or BPD-PH. Subgroup analysis among m/s BPD patients revealed that early PH (4.50; 1.61–12.58) and prolonged invasive ventilator care (> 28 days) (4.91; 1.02–23.68) increased the risk for late PH independently. In conclusion, EPIs with early PH at 4–7 PND should be monitored for BPD-associated late PH development.