bluuebunny/crossref_metadata_2025 huggingface dataset:
Nanosized V-Ce Oxides Supported on TiO2 as a Superior Catalyst for the Selective Catalytic Reduction of NO
Authors: Lu Long , Wang Xueman , Hu Chunhua , Liu Ying , Chen Xiongbo , Fang Ping , Chen Dingsheng , Cen Chaoping
Published: February 2020

Nanosized V-Ce oxides supported on TiO2 (VCT) were prepared and utilized in the low-temperature selective catalytic reduction (SCR) of NO with NH3. Compared with the other V-Ce oxides-based catalysts supported on Al2O3, ZrO2, and ZSM-5, VCT showed the best SCR activity in a low-temperature range. The NOx conversion of 90% could be achieved at 220 °C. Characterizations including X-ray diffraction (XRD), scanning election micrograph (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), temperature-programmed desorption with NH3 (NH3-TPD), and temperature-programmed reduction with H2 (H2-TPR) showed that V1.05Ce1/TiO2 exhibited a good dispersion of V2O5, enrichment of surface Ce3+ and chemical-absorbed oxygen, and excellent redox capacity and acidity, which resulted in the best SCR performance at low temperature.
Effect of Sodium Glucose Cotransporter 2 Inhibitors on Renal Function in Patients with Nonalcoholic Fatty Liver Disease and Type 2 Diabetes in Japan
Authors: Yano Kota , Seko Yuya , Takahashi Aya , Okishio Shinya , Kataoka Seita , Takemura Masashi , Okuda Keiichiroh , Mizuno Naoki , Taketani Hiroyoshi , Umemura Atsushi , Nishikawa Taichiro , Yamaguchi Kanji , Moriguchi Michihisa , Okanoue Takeshi , Itoh Yoshito
Published: February 2020

Sodium-glucose cotransporter-2 inhibitors (SGLT2I) have been reported to have renal-protective effects in patients with type 2 diabetes (T2DM). This a retrospective study aimed to evaluate the effect of SGLT2I on renal function in patients with nonalcoholic fatty liver disease (NAFLD) and T2DM. We analyzed 69 consecutive patients with a biopsy-proven NAFLD and T2DM with an estimated glomerular filtration rate (eGFR) >60 mL/min. Of these 69 patients, 22 received SGLT2I and 47 were treated without SGLT2I. Liver function and eGFR were analyzed at baseline and after three years. Body mass index, liver function and HbA1c improved significantly in both groups. In the total population, the median eGFR declined from 80.7 mL/min at the baseline to 74.9 mL/min at the end of follow-up. The median eGFR at the baseline/end of follow-up was 81.2/80.4 mL/min in patients treated with SGLT2I and 80.2/70.8 mL/min in patients treated without SGLT2I. Multivariate analysis identified an increased FIB-4 index with an odds ratio (OR) of 4.721, (p = 0.045) and SGLT2I treatment (OR 0.263, p = 0.033) as predictive factors for decreased eGFR. SGLT2I treatment has a protective effect on the renal function for NAFLD with T2DM. A long-term, randomized, controlled trial is warranted to confirm the renal protective effect of SGLT2I in NAFLD patients with T2DM.
Pancreatic Fibrosis and Chronic Pancreatitis: Mini-Review of Non-Histologic Diagnosis for Clinical Applications
Authors: Huang Chung-Tsui , Lin Cheng-Kuan , Lee Tzong-Hsi , Liang Yao-Jen
Published: February 2020

Pancreatic fibrosis is the dominant reversible pathological change and diagnostic factor in early chronic pancreatitis, defined by a mechanistic approach proposed in 2016. Main guidelines for chronic pancreatitis were published by the American Pancreas Association in 2014, the Japanese Society of Gastroenterology in 2015, and United European Gastroenterology in 2017. All three sets of guidelines mentioned that the staging of chronic pancreatitis is important but challenging. There are various image modalities for the non-histologic diagnosis of pancreatic fibrosis: (1) shear wave elastography, such as an acoustic radiation force impulse with a cut-off value of 1.4 m/s; (2) strain elastography using grades of strain; (3) endoscopic ultrasonography using the Rosemont criteria or endoscopic ultrasound criteria for early chronic pancreatitis proposed by the Japan Pancreas Society; (4) computed tomography using the Hounsfield scale or number of micro-calcifications; and (5) magnetic resonance imaging using the apparent diffusion coefficient and the T1w flash and T2w HASTE sequences. The clinical applications are to (1) evaluate pancreatic tumors and inflammatory disease; (2) monitor dyspepsia with early chronic pancreatitis; (3) monitor individuals with a high risk of pancreatic cancer; (4) analyze a fatty pancreas with fibrosis; (5) predict a fistula after pancreatic surgery; and (6) predict outcomes for chronic pancreatitis or pancreatic cancer. The selection of tools will be dependent on the clinical scenario. Conclusion: There are various modalities for the non-histologic diagnosis of pancreatic fibrosis. The selection of the optimal device will be dependent on the clinical scenario.
Transmission of X-linked Ovarian Cancer: Characterization and Implications
Authors: Etter John Lewis , Moysich Kirsten , Kohli Shaun , Lele Shashikant , Odunsi Kunle , Eng Kevin H.
Published: February 2020

We recently reported evidence that a strong, BRCA-independent locus on the X-chromosome may contribute to ovarian cancer predisposition in families ascertained from the Familial Ovarian Cancer Registry (Buffalo, NY, USA). While it has been estimated that approximately 20% of all ovarian cancer cases are hereditary, it is possible that a significant proportion of cases previously believed to be sporadic may, in fact, be X-linked. Such X-linked disease has a distinct pattern; it implies that a father will necessarily pass a risk allele to each of his daughters, increasing the prevalence of cancers clustered within a family. X-chromosome inactivation further influences the expression of X-linked alleles and may represent a novel target for screening and therapy. Herein, we review the current literature regarding X-linked ovarian cancer and interpret allele transmission-based models to characterize X-linked ovarian cancer and develop a framework for clinical and epidemiological familial ascertainment to inform the design of future studies.
Continuous Measurement of Three-Dimensional Root Canal Curvature Using Cone-Beam Computed and Micro-Computed Tomography: A Comparative Study
Authors: Kucher Michael , Dannemann Martin , Modler Niels , Haim Dominik , Hannig Christian , Weber Marie-Theres
Published: February 2020

The knowledge of root canal curvature is crucial regarding the prevention of ledge formation, root perforation and the possibility of endodontic instruments’ fracture during endodontic treatments. Therefore, a quantification method of the root canal curvature as well as the applicability of diagnostically relevant tomographic three-dimensional (3D) imaging data is necessary. Hereby, cone-beam computed tomography (CBCT) images and micro-computed tomography (µCT) data of distal root canals were analysed concerning the continuous three-dimensional curvature of human mandibular molars ( n = 50). The curvature of the canal’s three-dimensional centre line was determined by evaluating the tomographic images. The centroids of each root canal slice were identified and approximated by spline curves to obtain the centre line and therefore, its curvature. Comparing the results evaluated from CBCT and µCT images, minimum radii of curvature of 2.6 mm and 2.1 mm were determined, respectively. The observation of the centre line demonstrated the requirement of the three-dimensional imaging data from CBCT and µCT for a reliable curvature analysis. Conclusively, the evaluation of CBCT and µCT images results in comparable radii of curvature. Thus, the application of the introduced method in combination with CBCT applied to patient cases could offer an important preliminary diagnostical step to prevent endodontic treatment complications.
Position Estimation of a PMSM in an Electric Propulsion Ship System Based on High-Frequency Injection
Authors: Bai Hongfen
Published: February 2020

To improve the operating performance of electric propulsion ships, the permanent magnet synchronous motor is commonly used as the propulsion motor. Additionally, position estimation without sensors can further improve the application range of the propulsion motor and the estimated results can represent the redundancy of measured values from mechanical sensors. In this paper, the high-frequency (HF) injection algorithm combined with the second-order generalized integrator (SOGI) is presented on the basis of analyzing the structure of the electric propulsion ship and the vector control of the motors. The position and rotor speed were estimated accurately by the approximate calculation of q-axis currents directly related to the rotor position. Moreover, the harmonics in the estimated position were effectively reduced by the introduction of the second-order generalized integrator. Then, the rotor position estimation algorithm was verified in MATLAB/Simulink by choosing different low speeds including speed reversal, increasing speed, and increasing load torque. Finally, the correctness of the proposed improved high-frequency injection algorithm based on the second-order generalized integrator was verified by the experimental propulsion permanent magnet synchronous motor (PMSM) system at low speed.
Multiport Isolated link with Current-Fed Z-Source Converters to Manage Power Imbalance in PV Applications
Authors: Sandoval Jose M. , Cardenas Victor , Barrios Manuel A. , Garcia Mario Gonzalez , Alcala Janeth
Published: February 2020

In order to address power imbalance in large-scale PV systems, this paper presents a multiport isolated medium-frequency (MF) link to process different power levels from PV arrays, using current-fed Z-source inverters (CZSI) modules to drive the energy from the PV array to the MF link in a single power stage. The MF link provides the galvanic isolation required by many codes and standards to integrate a system to the grid. The multiport configuration of the MF link allows the system to process different power levels on each primary port and, being all ports magnetically coupled, provide balanced power levels on secondary ports which can be used for multilevel converters applications. Additionally, the current-fed version of the Z-source topology offers natural protection against short-circuit damage enhancing reliability of the system. The analysis of CZSI cells and the study of asymmetrical power levels transference, as well as simulation and experimental results are presented.
Performance Testing on Vector vs. Raster Map Tiles—Comparative Study on Load Metrics
Authors: Netek Rostislav , Masopust Jan , Pavlicek Frantisek , Pechanec Vilem
Published: February 2020

Recent developments in web map applications have widely affected how background maps are rendered. Raster tiles are currently considered as a regular solution, while the use of vector tiles is becoming more widespread. This article describes an experiment to test both raster and vector tile methods. The concept behind raster tiles is based on pre-generating an original dataset including a customized symbology and style. All tiles are generated according to a standardized scheme. This method has a few disadvantages: if any change in the dataset is required, the entire tile-generating process must be redone. Vector tiles manipulate vector objects. Only vector geometry is stored on the server, while symbology, rendering, and defining zoom levels run on the client-side. This method simplifies changing symbology or topology. Based on eight pilot studies, performance testing on loading time, data size, and the number of requests were performed. The observed results provide a comprehensive comparison according to specific interactions. More data, but only one or two tiles, were downloaded for vector tiles in zoom and move interactions, while 40 tiles were downloaded for raster tiles for the same interactions. Generally, the WebP format downloaded about three times fewer data than Portable Network Graphics (PNG).
Role of Mesenchymal Stromal Cells as Therapeutic Agents: Potential Mechanisms of Action and Implications in Their Clinical Use
Authors: Jimenez-Puerta Gonzalo , Marchal Juan , López-Ruiz Elena , Gálvez-Martín Patricia
Published: February 2020

Due to the great therapeutic interest that involves the translation of mesenchymal stromal cells (MSCs) into clinical practice, they have been widely studied as innovative drugs, in order to treat multiple pathologies. MSC-based cell therapy involves the administration of MSCs either locally or systemically into the receptor body where they can traffic and migrate towards the affected tissue and participate in the process of healing. The therapeutic effects of MSCs compromise of different mechanisms such as the functional integration of differentiated MSCs into diseased host tissue after transplantation, their paracrine support, and their impact on the regulation of both the innate and the acquired immune system. Here, we establish and provide recent advances about the principal mechanisms of action through which MSCs can perform their activity and effect as a therapeutic tool. The purpose of this review is to examine and discuss the MSCs capacity of migration, their paracrine effect, as well as MSC-mediated modifications on immune cell responses.
Combined Brain-Heart Magnetic Resonance Imaging in Autoimmune Rheumatic Disease Patients with Cardiac Symptoms: Hypothesis Generating Insights from a Cross-Sectional Study
Authors: Markousis-Mavrogenis George , Mitsikostas Dimos D. , Koutsogeorgopoulou Loukia , Dimitroulas Theodoros , Katsifis Gikas , Argyriou Panayiotis , Apostolou Dimitrios , Velitsista Stella , Vartela Vasiliki , Manolopoulou Dionysia , Tektonidou Maria G. , Kolovou Genovefa , Kitas George D. , Sfikakis Petros P. , Mavrogeni Sophie I.
Published: February 2020

Background: Autoimmune rheumatic diseases (ARDs) may affect both the heart and the brain. However, little is known about the interaction between these organs in ARD patients. We asked whether brain lesions are more frequent in ARD patients with cardiac symptoms compared with non-ARD patients with cardiovascular disease (CVD). Methods: 57 ARD patients with mean age of 48 ± 13 years presenting with shortness of breath, chest pain, and/or palpitations, and 30 age-matched disease-controls with non-autoimmune CVD, were evaluated using combined brain–heart magnetic resonance imaging (MRI) in a 1.5T system. Results: 52 (91%) ARD patients and 16 (53%) controls had white matter hyperintensities (p < 0.001) in at least one brain area (subcortical/deep/periventricular white matter, basal ganglia, pons, brainstem, or mesial temporal lobe). Only the frequency and number of subcortical and deep white matter lesions were significantly greater in ARD patients (p < 0.001 and 0.014, respectively). ARD vs. control status was the only independent predictor of having any brain lesion. Specifically for deep white matter lesions, each increase in ECV independently predicted a higher number of lesions [odds ratio (95% confidence interval): 1.16 (1.01–1.33), p = 0.031] in ordered logistic regression. Penalized logistic regression selected only ARD vs. control status as the most important feature for predicting whether brain lesions were present on brain MRI (odds ratio: 5.46, marginal false discovery rate = 0.011). Conclusions: Subclinical brain involvement was highly prevalent in this cohort of ARD patients and was mostly independent of the severity of cardiac involvement. However, further research is required to determine the clinical relevance of these findings.
Extracorporeal Shockwave Therapy Treatment in Upper Limb Diseases: A Systematic Review
Authors: Testa Gianluca , Vescio Andrea , Perez Stefano , Consoli Alberto , Costarella Luciano , Sessa Giuseppe , Pavone Vito
Published: February 2020

Background: Rotator cuff tendinopathy (RCT), subacromial impingement (SAIS), and medial (MEP) and lateral (LEP) epicondylitis are the most common causes of upper limb pain caused by microtrauma and degeneration. There are several therapeutic choices to manage these disorders: extracorporeal shockwave therapy (ESWT) has become a valuable option. Methods: A systematic review of two electronic medical databases was performed by two independent authors, using the following inclusion criteria: RCT, SAIS, MEP, and LEP, ESWT therapy without surgical treatment, with symptoms duration more than 2 months, and at least 6 months of follow-up. Studies of any level of evidence, reporting clinical results, and dealing with ESWT therapy and RCT, SAIS, MEP, and LEP were included. Results: A total of 822 articles were found. At the end of the first screening, following the previously described selection criteria, we selected 186 articles eligible for full-text reading. Ultimately, after full-text reading, and reference list check, we selected 26 articles following previously written criteria. Conclusions: ESWT is a safe and effective treatment of soft tissue diseases of the upper limbs. Even in the minority cases when unsatisfied results were recorded, high energy shockwaves were nevertheless suggested in prevision of surgical treatment.
GDNF, A Neuron-Derived Factor Upregulated in Glial Cells during Disease
Authors: Duarte Azevedo Marcelo , Sander Sibilla , Tenenbaum Liliane
Published: February 2020

In a healthy adult brain, glial cell line-derived neurotrophic factor (GDNF) is exclusively expressed by neurons, and, in some instances, it has also been shown to derive from a single neuronal subpopulation. Secreted GDNF acts in a paracrine fashion by forming a complex with the GDNF family receptor α1 (GFRα1), which is mainly expressed by neurons and can act in cis as a membrane-bound factor or in trans as a soluble factor. The GDNF/GFRα1 complex signals through interactions with the “rearranged during transfection” (RET) receptor or via the neural cell adhesion molecule (NCAM) with a lower affinity. GDNF can also signal independently from GFRα1 by interacting with syndecan-3. RET, which is expressed by neurons involved in several pathways (nigro–striatal dopaminergic neurons, motor neurons, enteric neurons, sensory neurons, etc.), could be the main determinant of the specificity of GDNF’s pro-survival effect. In an injured brain, de novo expression of GDNF occurs in glial cells. Neuroinflammation has been reported to induce GDNF expression in activated astrocytes and microglia, infiltrating macrophages, nestin-positive reactive astrocytes, and neuron/glia (NG2) positive microglia-like cells. This disease-related GDNF overexpression can be either beneficial or detrimental depending on the localization in the brain and the level and duration of glial cell activation. Some reports also describe the upregulation of RET and GFRα1 in glial cells, suggesting that GDNF could modulate neuroinflammation.
Inflammatory Targets in Diabetic Nephropathy
Authors: Donate-Correa Javier , Luis-Rodríguez Desirée , Martín-Núñez Ernesto , Tagua Víctor G. , Hernández-Carballo Carolina , Ferri Carla , Rodríguez-Rodríguez Ana Elena , Mora-Fernández Carmen , Navarro-González Juan F.
Published: February 2020

One of the most frequent complications in patients with diabetes mellitus is diabetic nephropathy (DN). At present, it constitutes the first cause of end stage renal disease, and the main cause of cardiovascular morbidity and mortality in these patients. Therefore, it is clear that new strategies are required to delay the development and the progression of this pathology. This new approach should look beyond the control of traditional risk factors such as hyperglycemia and hypertension. Currently, inflammation has been recognized as one of the underlying processes involved in the development and progression of kidney disease in the diabetic population. Understanding the cascade of signals and mechanisms that trigger this maladaptive immune response, which eventually leads to the development of DN, is crucial. This knowledge will allow the identification of new targets and facilitate the design of innovative therapeutic strategies. In this review, we focus on the pathogenesis of proinflammatory molecules and mechanisms related to the development and progression of DN, and discuss the potential utility of new strategies based on agents that target inflammation.
Whole Genome Sequencing Results Associated with Minimum Inhibitory Concentrations of 14 Anti-Tuberculosis Drugs among Rifampicin-Resistant Isolates of Mycobacterium Tuberculosis from Iran
Authors: Kardan-Yamchi Jalil , Kazemian Hossein , Battaglia Simone , Abtahi Hamidreza , Rahimi Foroushani Abbas , Hamzelou Gholamreza , Cirillo Daniela Maria , Ghodousi Arash , Feizabadi Mohammad Mehdi
Published: February 2020

Accurate and timely detection of drug resistance can minimize the risk of further resistance development and lead to effective treatment. The aim of this study was to determine the resistance to first/second-line anti-tuberculosis drugs in rifampicin/multidrug-resistant Mycobacterium tuberculosis (RR/MDR-MTB) isolates. Molecular epidemiology of strains was determined using whole genome sequencing (WGS)-based genotyping. A total of 35 RR/MDR-MTB isolates were subjected to drug susceptibility testing against first/second-line drugs using 7H9 Middlebrook in broth microdilution method. Illumina technology was used for paired-end WGS applying a Maxwell 16 Cell DNA Purification kit and the NextSeq platform. Data analysis and single nucleotide polymorphism calling were performed using MTBseq pipeline. The genome-based resistance to each drug among the resistant phenotypes was as follows: rifampicin (97.1%), isoniazid (96.6%), ethambutol (100%), levofloxacin (83.3%), moxifloxacin (83.3%), amikacin (100%), kanamycin (100%), capreomycin (100%), prothionamide (100%), D-cycloserine (11.1%), clofazimine (20%), bedaquiline (0.0%), and delamanid (44.4%). There was no linezolid-resistant phenotype, and a bedaquiline-resistant strain was wild type for related genes. The Beijing, Euro-American, and Delhi-CAS were the most populated lineage/sublineages. Drug resistance-associated mutations were mostly linked to minimum inhibitory concentration results. However, the role of well-known drug-resistant genes for D-cycloserine, clofazimine, bedaquiline, and delamanid was found to be more controversial.
Determination of a Hysteresis Model Parameters with the Use of Different Evolutionary Methods for an Innovative Hysteresis Model
Authors: Jesenik Marko , Mernik Marjan , Trlep Mladen
Published: February 2020

For precise modeling of electromagnetic devices, we have to model material hysteresis. A Genetic Algorithm, Differential Evolution with three different strategies, teaching–learning-based optimization and Artificial Bee Colony, were used for testing seven different modified mathematical expressions, and the best combination of mathematical expression and solving method was used for hysteresis modeling. The parameters of the hysteresis model were determined based on the measured major hysteresis loop and first-order reversal curves. The model offers a simple determination of the magnetization procedure in the areas between measured curves, with the only correction of two parameters based on only two known points in the magnetization process. It was tested on two very different magnetic materials, and results show good agreement between the measured and calculated curves. The calculated curves between the measured curves have correct shapes. The main difference between our model and other models is that, in our model, each measured curve, major and reversal, is described with different parameters. The magnetization process between measured curves is described according to the nearest measured curve, and this ensures the best fit for each measured curve. In other models, there is mainly only one curve, a major hysteresis or magnetization curve, used for the determination of the parameters, and all other curves are then dependent on this curve. Results confirm that the evolutionary optimization method offers a reliable procedure for precise determination of the parameters.
On C-To-R-Based Iteration Methods for a Class of Complex Symmetric Weakly Nonlinear Equations
Authors: Zeng Min-Li , Zhang Guo-Feng
Published: February 2020

To avoid solving the complex systems, we first rewrite the complex-valued nonlinear system to real-valued form (C-to-R) equivalently. Then, based on separable property of the linear and the nonlinear terms, we present a C-to-R-based Picard iteration method and a nonlinear C-to-R-based splitting (NC-to-R) iteration method for solving a class of large sparse and complex symmetric weakly nonlinear equations. At each inner process iterative step of the new methods, one only needs to solve the real subsystems with the same symmetric positive and definite coefficient matrix. Therefore, the computational workloads and computational storage will be saved in actual implements. The conditions for guaranteeing the local convergence are studied in detail. The quasi-optimal parameters are also proposed for both the C-to-R-based Picard iteration method and the NC-to-R iteration method. Numerical experiments are performed to show the efficiency of the new methods.
New Processes and Machine Tools for Advanced Metal Alloys
Authors: de Lacalle Luis Norberto López , Celaya Ainhoa
Published: February 2020

Advanced materials are crucial for the development of many industrial sectors such as aerospace, automotive, energy, among others [...]
High Prevalence of Strongyloides among South Asian Migrants in Primary Care―Associations with Eosinophilia and Gastrointestinal Symptoms
Authors: Baker Elinor Chloe , Ming Damien K. , Choudhury Yasmin , Rahman Shahedur , Smith Philip J. , Muñoz Jose , Chiodini Peter L. , Griffiths Chris J. , Whitty Christopher J. M. , Brown Michael
Published: February 2020

Gastrointestinal (GI) symptoms are a frequent reason for primary care consultation, and common amongst patients with strongyloidiasis. We conducted a prospective cohort and nested case control study in East London to examine the predictive value of a raised eosinophil count or of GI symptoms, for Strongyloides infection in South Asian migrants. We included 503 patients in the final analyses and all underwent a standardised GI symptom questionnaire, eosinophil count and Strongyloides serology testing. Positive Strongyloides serology was found in 33.6% in the eosinophilia cohort against 12.5% in the phlebotomy controls, with adjusted odds ratio of 3.54 (95% CI 1.88–6.67). In the GI symptoms cohort, 16.4% were seropositive but this was not significantly different compared with controls, nor were there associations between particular symptoms and Strongyloidiasis. Almost a third (35/115) of patients with a positive Strongyloides serology did not have eosinophilia at time of testing. Median eosinophil count declined post-treatment from 0.5 cells × 109/L (IQR 0.3–0.7) to 0.3 (0.1–0.5), p < 0.001. We conclude Strongyloides infection is common in this setting, and the true symptom burden remains unclear. Availability of ivermectin in primary care would improve access to treatment. Further work should clarify cost-effectiveness of screening strategies for Strongyloides infection in UK migrant populations.
Cytokinins Are Abundant and Widespread among Insect Species
Authors: Andreas Peter , Kisiala Anna , Emery R. J. Neil , De Clerck-Floate Rosemarie , Tooker John F. , Price Peter W. , Miller III Donald G. , Chen Ming-Shun , Connor Edward F.
Published: February 2020

Cytokinins (CKs) are a class of compounds that have long been thought to be exclusively plant growth regulators. Interestingly, some species of phytopathogenic bacteria and fungi have been shown to, and gall-inducing insects have been hypothesized to, produce CKs and use them to manipulate their host plants. We used high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-MS/MS) to examine concentrations of a wide range of CKs in 17 species of phytophagous insects, including gall- and non-gall-inducing species from all six orders of Insecta that contain species known to induce galls: Thysanoptera, Hemiptera, Lepidoptera, Coleoptera, Diptera, and Hymenoptera. We found CKs in all six orders of insects, and they were not associated exclusively with gall-inducing species. We detected 24 different CK analytes, varying in their chemical structure and biological activity. Isoprenoid precursor nucleotide and riboside forms of trans-zeatin (tZ) and isopentenyladenine (iP) were most abundant and widespread across the surveyed insect species. Notably, the observed concentrations of CKs often markedly exceeded those reported in plants suggesting that insects are synthesizing CKs rather than obtaining them from the host plant via tissue consumption, compound sequestration, and bioaccumulation. These findings support insect-derived CKs as means for gall-inducing insects to manipulate their host plant to facilitate cell proliferation, and for both gall- and non-gall-inducing insects to modify nutrient flux and plant defenses during herbivory. Furthermore, wide distribution of CKs across phytophagous insects, including non-gall-inducing species, suggests that insect-borne CKs could be involved in manipulation of source-sink mechanisms of nutrient allocation to sustain the feeding site and altering plant defensive responses, rather than solely gall induction. Given the absence of any evidence for genes in the de novo CK biosynthesis pathway in insects, we postulate that the tRNA-ipt pathway is responsible for CK production. However, the unusually high concentrations of CKs in insects, and the tendency toward dominance of their CK profiles by tZ and iP suggest that the tRNA-ipt pathway functions differently and substantially more efficiently in insects than in plants.
Post-Silking Shading Stress Affects Leaf Nitrogen Metabolism of Spring Maize in Southern China
Authors: Wang Jue , Shi Kai , Lu Weiping , Lu Dalei
Published: February 2020

Lower sunlight caused by overcast skies from June to July in Southern China is one of the main environmental stresses that frequently occur and affect the post-silking growth and grain development of spring maize. In this study, a field trial involving four maize hybrids as materials was conducted to investigate the effects of post-silking shading stress (30% and 50% light deprivation) on leaf nitrogen metabolism and biomass accumulation during maize growing seasons in 2016 and 2017. Results indicated that 30% and 50% shading stress caused the grain yield to decrease by 47.3% and 69.6%, respectively. Plant post-silking biomass accumulation was decreased by shading, whereas the translocation from pre-silking assimilates in the vegetative organs was increased by shading. This change was sharply observed when the plants were deprived of more sunlight intensity. The leaf relative chlorophyll (soil and plant analyzer development (SPAD) value) and soluble protein contents were considerably decreased by shading under 50% light deprivation condition. The activities of nitrate reductase, glutamine synthetase and glutamate synthase that are involved in nitrogen metabolism were downregulated by shading stresses. In conclusion, nitrogen metabolism was disturbed by shading, which induced the decrease in post-silking dry matter accumulation, ultimately resulting in grain yield loss.
Flavones Produced by Mulberry Flavone Synthase Type I Constitute a Defense Line against the Ultraviolet-B Stress
Authors: Li Han , Li Dong , Yang Zhen , Zeng Qiwei , Luo Yiwei , He Ningjia
Published: February 2020

Flavones, one of the largest classes of flavonoids in plants, have a variety of bioactivities and participate in the resistance response of plants to biotic and abiotic stresses. However, flavone synthase (FNS), the key enzyme for flavone biosynthesis, has not yet been characterized in mulberry. In this study, we report that the leaves of certain mulberry cultivars, namely BJ7, PS2, and G14, are rich in flavones. We identified a Fe2+/2-oxoglutarate-dependent dioxygenase from Morus notabilis (MnFNSI) that shows the typical enzymatic activity of a FNSI-type enzyme, and directly converts eriodictyol and naringenin into their corresponding flavones. Overexpression of MnFNSI in tobacco increased the flavones contents in leaves and enhanced the tolerance of tobacco to ultraviolet-B (UV-B) stress. We found that mulberry cultivars with higher flavones contents exhibit less UV-B induced damage after a UV-B treatment. Accordingly, our findings demonstrate that MnFNSI, a FNSI-type enzyme, is involved in the biosynthesis of flavones, which provide protection against UV-B radiation. These results lay the foundation for obtaining mulberry germplasm resources that are more tolerant to UV-B stress and richer in their nutritional value.
Thermodynamic Performance Analysis of Hydrofluoroolefins (HFO) Refrigerants in Commercial Air-Conditioning Systems for Sustainable Environment
Authors: Farooq Muhammad , Hamayoun Ahsan , Naqvi Muhammad , Nawaz Saad , Usman Muhammad , Naqvi Salman Raza , Imran Muhammad , Nadeem Rida , Razi Allah , Turan Ahmet , Pettinau Alberto , Andresen John M.
Published: February 2020

Global warming is one of most severe environmental concerns that our planet is facing today. One of its causes is the previous generation of refrigerants that, upon release, remain in the atmosphere for longer periods and contribute towards global warming. This issue could potentially be solved by replacing the previous generation’s high global warming potential (GWP) refrigerants with environmentally friendly refrigerants. This scenario requires an analysis of new refrigerants for a comparison of the thermodynamic properties of the previously used refrigerants. In the present research, a numerical study was conducted to analyze the thermodynamic performance of specifically low GWP hydrofluoroolefens (HFO) refrigerants for an actual vapor compression refrigeration cycle (VCRC) with a constant degree of 3 K superheat. The output parameters included the refrigeration effect, compressor work input, the coefficient of performance (COP), and the volumetric refrigeration capacity (VRC), all of which were calculated by varying the condenser pressure from 6 to 12 bars and vapor pressure from 0.7 to 1.9 bars. Results showed that R1234ze(Z) clearly possessed the desired thermodynamic performance. The drop in refrigeration effect for R1234ze(Z) was merely 14.6% less than that of R134a at a 12 bar condenser pressure; this was minimum drop among candidate refrigerants. The drop in the COP was the minimum for R1234ze(Z)—5.1% less than that of R134a at a 9 bar condenser pressure and 4.7% less than that of R134a at a 1.9 bar evaporator pressure, whereas the COP values of the other refrigerants dropped more drastically at higher condenser pressures. R1234ze(Z) possessed favorable thermodynamic characteristics, with a GWP of 7, and it can serve as an alternative refrigerant for refrigeration systems for a sustainable environment.
Metabolite Profiling of Aquilaria malaccensis Leaf Extract Using Liquid Chromatography-Q-TOF-Mass Spectrometry and Investigation of Its Potential Antilipoxygenase Activity In-Vitro
Authors: Eissa Manar A. , Hashim Yumi Z. H-Y. , El-Kersh Dina M. , Abd-Azziz Saripah S. S. , Salleh Hamzah Mohd. , Isa Muhammad Lokman Md. , Abd Warif Nor Malia
Published: February 2020

The Aquilaria malaccensis species of the genus Aquilaria is an abundant source of agarwood resin and many bioactive phytochemicals. Recent data regarding the chemical constituents and biological activities of Aquilaria leaves led us to attempt to qualitatively profile the metabolites of Aquilaria malaccensis leaves from a healthy, noninoculated tree through phytochemical screening, GC-MS, and LC/Q-TOF-MS. The present work is also the first to report the antilipoxygenase activity of A. malaccensis leaves from healthy noninoculated tree and investigate its toxicity on oral mucosal cells. A total of 53 compounds were tentatively identified in the extract, some of which have been described in literature as exhibiting anti-inflammatory activity. A number of compounds were identified for the first time in the extract of A. malaccensis leaf, including quercetin, quercetin-O-hexoside, kaempferol-O-dirhamnoside, isorhamnetin-O-hexoside, syringetin-O-hexoside, myricetin, tetrahydroxyflavanone, hesperetin, sissotrin, and lupeol. The antilipoxygenase assay was used to determine the lipoxygenase (LOX) inhibitory potential of the extract, while a WST-1 assay was conducted to investigate the effect of the extract on oral epithelial cells (OEC). The extract implied moderate anti-LOX activity with IC50 value of 71.6 µg/mL. Meanwhile, the cell viability of OEC ranged between 92.55% (10 µg/mL)–76.06% ± (100 µg/mL) upon treatment, indicating some potential toxicity risks. The results attained encourage future studies of the isolation of bioactive compounds from Aquilaria malaccensis leaves, as well as further investigation on the anti-inflammatory mechanisms and toxicity associated with their use.
Quantifying the Benefit of a Dynamic Performance Assessment of WWTP
Authors: Revollar Silvana , Meneses Montse , Vilanova Ramón , Vega Pastora , Francisco Mario
Published: February 2020

In this work a comprehensive analysis of the environmental impact of the operation of a wastewater treatment plant (WWTP) using different control strategies is carried out considering the dynamic evolution of some environmental indicators and average operation costs. The selected strategies are PI (proportional integral) control schemes such as dissolved oxygen control in the aerobic zone (DO control), DO control and nitrates control in the anoxic zone (DO + NO control) and regulation of ammonium control at the end of aerobic zone (Cascade SNHSP) commonly used in WWTPs to maintain the conditions that ensure the desired effluent quality in a variable influent scenario. The main novelty of the work is the integration of potential insights into environmental impact from the analysis of dynamic evolution of environmental indicators at different time scales. The consideration of annual, bimonthly and weekly temporal windows to evaluate performance indicators makes it possible to capture seasonal effects of influent disturbances and control actions on environmental costs of wastewater treatment that are unnoticed in the annual-based performance evaluation. Then, in the case of periodic events, it is possible to find solutions to improve operation by the adjustment of the control variables in specific periods of time along the operation horizon. The analysis of the annual average and dynamic profiles (weekly and bimonthly) of environmental indicators showed that ammonium-based control (Cascade SNHSP) produce the best compromise solution between environmental and operation costs compared with DO control and DO + NO control. An alternative control strategy, named SNHSP var Qcarb var, has been defined considering a sequence of changes on ammonium set-point (SNHSP) and carbon dosage (Qcarb) on different temporal windows. It is compared with DO control considering weekly and bimonthly profiles and annual average values leading to the conclusion that both strategies, Cascade SNHSP and SNHSP var Qcarb var, produce an improvement of dynamic and annual average environmental performance and operation costs, but benefits of Cascade SNHSP strategy are associated with reduction of electricity consumption and emissions to water, while SNHSP var Qcarb var strategy reduces electricity consumption, use of chemicals (reducing external carbon dosage) and operation costs.
Use of Data Analysis Methods in Dental Publications: Is There Evidence of a Methodological Change?
Authors: Nieminen Pentti , Vähänikkilä Hannu
Published: February 2020

Objectives: To evaluate how data analysis methods in dental studies have changed in recent years. Methods: A total of 400 articles published in 2010 and 2017 in five dental journals, Journal of Dental Research, Caries Research, Community Dentistry and Oral Epidemiology, Journal of Dentistry, and Acta Odontologica Scandinavica, were analyzed. The study characteristics and the reporting of data analysis techniques were systematically identified. Results: The statistical intensity of the dental journals did not change from 2010 to 2017. Dental researchers did not adopt the data mining, machine learning, or Bayesian approaches advocated in the computer-oriented methodological literature. The determination of statistical significance was the most generally used method for conducting research in both 2010 and 2017. Observational study designs were more common in 2017. Insufficient and incomplete descriptions of statistical methods were still a serious problem. Conclusion: The stabilization of statistical intensity in the literature suggests that papers applying highly computationally complex data analysis methods have not meaningfully contributed to dental research or clinical care. Greater rigor is required in reporting the methods in dental research articles, given the current pervasiveness of failure to describe the basic techniques used.
Oscillating Neutrinos and Majorana Neutrino Masses
Authors: Fritzsch Harald
Published: February 2020

We discuss the mass matrices with texture zeros for the quarks and leptons. The flavor mixing angles for the quarks are functions of the quark masses and can be calculated. The results agree with the experimental data. The texture zero mass matrices for the leptons and the see-saw mechanism are used to derive relations between the matrix elements of the lepton mixing matrix and the ratios of the neutrino masses. Using the measured neutrino mass differences, the neutrino masses can be calculated. The neutrinoless double beta decay is discussed. The effective Majorana neutrino mass, describing the neutrinoless double beta decay, can be calculated—it is about 4.6 meV. The present experimental limit is at least twenty times larger.
Plasmablast, Memory B Cell, CD4+ T Cell, and Circulating Follicular Helper T Cell Responses to a Non-Replicating Modified Vaccinia Ankara Vaccine
Authors: Anderson Evan J. , Lai Lilin , Wrammert Jens , Kabbani Sarah , Xu Yongxian , Priyamvada Lalita , Hill Heather , Goll Johannes B. , Jensen Travis L. , Kao Carol , Yildirim Inci , Rouphael Nadine , Jackson Lisa , Mulligan Mark J.
Published: February 2020

Background: Vaccinia is known to induce antibody and cellular responses. Plasmablast, circulating follicular helper T (cTFH) cells, cytokine-expressing CD4 T cells, and memory B cells were compared between subcutaneous (SC) and needle-free jet injection (JI) recipients of non-replicating modified vaccinia Ankara (MVA) vaccine. Methods: Vaccinia-naïve adults received MVA SC or by JI on Days 1 and 29. Vaccinia-specific antibodies were quantified by plaque reduction neutralization test (PRNT) and enzyme-linked immunosorbent assay. Plasmablast, cTFH, and cytokine-expressing CD4 T cells were assessed on Days 1, 8, 15, 29, 36, 43 (cTFH and CD4+ only) and 57. Memory B cells were measured on Days 1 and 57. Results: Of the 36 enrolled subjects, only 22 received both vaccinations and had evaluable specimens after the second vaccine. Plasmablasts peaked one week after each vaccine. Day 15 plasmablasts correlated with peak PRNT titers. cTFH peaked on Days 8 and 36 and correlated with Day 36 plasmablasts. CD4+ peaked at Day 29 and one-third produced ≥2 cytokines. Day 57 memory B cells ranged from 0.1% to 0.17% of IgG-secreting B cells. Conclusions: This study provides insights into the cellular responses to non-replicating MVA, currently used as a vector for a variety of novel vaccines.
Transcriptomic Profiling of Tumor-Infiltrating CD4+TIM-3+ T Cells Reveals Their Suppressive, Exhausted, and Metastatic Characteristics in Colorectal Cancer Patients
Authors: Sasidharan Nair Varun , M Toor Salman , Z Taha Rowaida , Ahmed Ayman A , Kurer Mohamed A , Murshed Khaled , Soofi Madiha E , Ouararhni Khalid , M. Alajez Nehad , Abu Nada Mohamed , Elkord Eyad
Published: February 2020

T cell immunoglobulin mucin-3 (TIM-3) is an immune checkpoint identified as one of the key players in regulating T-cell responses. Studies have shown that TIM-3 is upregulated in the tumor microenvironment (TME). However, the precise role of TIM-3 in colorectal cancer (CRC) TME is yet to be elucidated. We performed phenotypic and molecular characterization of TIM-3+ T cells in the TME and circulation of CRC patients by analyzing tumor tissues (TT, TILs), normal tissues (NT, NILs), and peripheral blood mononuclear cells (PBMC). TIM-3 was upregulated on both CD4+ and CD3+CD4− (CD8+) TILs. CD4+TIM-3+ TILs expressed higher levels of T regulatory cell (Tregs)-signature genes, including FoxP3 and Helios, compared with their TIM-3− counterparts. Transcriptomic and ingenuity pathway analyses showed that TIM-3 potentially activates inflammatory and tumor metastatic pathways. Moreover, NF-κB-mediated transcription factors were upregulated in CD4+TIM-3+ TILs, which could favor proliferation/invasion and induce inflammatory and T-cell exhaustion pathways. In addition, we found that CD4+TIM-3+ TILs potentially support tumor invasion and metastasis, compared with conventional CD4+CD25+ Tregs in the CRC TME. However, functional studies are warranted to support these findings. In conclusion, this study discloses some of the functional pathways of TIM-3+ TILs, which could improve their targeting in more specific therapeutic approaches in CRC patients.
Statin Drugs Plus Th1 Cytokines Potentiate Apoptosis and Ras Delocalization in Human Breast Cancer Lines and Combine with Dendritic Cell-Based Immunotherapy to Suppress Tumor Growth in a Mouse Model of HER-2pos Disease
Authors: Oechsle Crystal M. , Showalter Loral E. , Novak Colleen M. , Czerniecki Brain J. , Koski Gary K.
Published: February 2020

A dendritic cell-based, Type 1 Helper T cell (Th1)-polarizing anti-Human Epidermal Growth Factor Receptor-2 (HER-2) vaccine supplied in the neoadjuvant setting eliminates disease in up to 30% of recipients with HER-2-positive (HER-2pos) ductal carcinoma in situ (DCIS). We hypothesized that drugs with low toxicity profiles that target signaling pathways critical for oncogenesis may work in conjunction with vaccine-induced immune effector mechanisms to improve efficacy while minimizing side effects. In this study, a panel of four phenotypically diverse human breast cancer lines were exposed in vitro to the combination of Th1 cytokines Interferon-gamma (IFN-γ) and Tumor Necrosis Factor-alpha (TNF-α) and lipophilic statins. This combination was shown to potentiate multiple markers of apoptotic cell death. The combination of statin drugs and Th1 cytokines minimized membrane K-Ras localization while maximizing levels in the cytoplasm, suggesting a possible means by which cytokines and statin drugs might cooperate to maximize cell death. A combined therapy was also tested in vivo through an orthotopic murine model using the neu-transgenic TUBO mammary carcinoma line. We showed that the combination of HER-2 peptide-pulsed dendritic cell (DC)-based immunotherapy and simvastatin, but not single agents, significantly suppressed tumor growth. Consistent with a Th1 cytokine-dependent mechanism, parenterally administered recombinant IFN-γ could substitute for DC-based immunotherapy, likewise inhibiting tumor growth when combined with simvastatin. These studies show that statin drugs can amplify a DC-induced effector mechanism to improve anti-tumor activity.