bluuebunny/crossref_metadata_2025 huggingface dataset:
Chip for dielectrophoretic microbial capture, separation and detection II: experimental study
Authors: Weber Monika U , Petkowski Janusz J , Weber Robert E , Krajnik Bartosz , Stemplewski Slawomir , Panek Marta , Dziubak Tomasz , Mrozinska Paulina , Piela Anna , Paluch Emil
Published: January 2023

In our previous paper we have modelled a dielectrophoretic force (DEP) and cell particle behavior in a microfluidic channel (Weber MU et al 2023 Chip for dielectrophoretic microbial capture, separation and detection I: theoretical basis of electrode design Nanotechnology this issue). Here we test and confirm the results of our modeling work by experimentally validating the theoretical design constraints of the ring electrode architecture. We have compared and tested the geometry and particle capture and separation performance of the two separate electrode designs (the ring and dot electrode structures) by investigating bacterial motion in response to the applied electric field. We have quantitatively evaluated the electroosmosis (EO) to positive DEP (PDEP) transition in both electrode designs and explained the differences in capture efficiency of the ring and dot electrode systems. The ring structure shows 99% efficiency of bacterial capture both for PDEP and for EO. Moreover, the ring structure shows an over 200 faster bacterial response to the electric field. We have also established that the ring electrode architecture, with appropriate structure periodicity and spacing, results in efficient capture and separation of microbial cells. We have identified several critical design constraints that are required to achieve high efficiency bacterial capture. We have established that the spacing between consecutive DEP traps smaller than the length of the depletion zone will ensure that the DEP force dominates bacterial motion over motility and Brownian motion.
Alterations in Vessel Hemodynamics Across Uncomplicated Pregnancy
Authors: Kuate Defo Alvin , Daskalopoulou Stella S
Published: January 2023

Pregnancy is marked by the onset of rapid hemodynamic alterations in order to accommodate the needs of the developing fetus. Arterial stiffness is an independent predictor of cardiovascular events and mortality, and its measurement in clinical practice has been recommended. It follows a U-shaped curve in uncomplicated pregnancy, decreasing to a nadir in mid-pregnancy and rising at term. Systemic vasodilation occurs due to elevated nitric oxide, prostacyclin, endothelium-derived hyperpolarizing factor, estrogen, progesterone, and relaxin. Vascular resistance decreases to a nadir in mid-pregnancy, while endothelial function is enhanced starting in the first trimester. Plasma volume increases by about 50%, and total red blood cell mass increases by up to 40%. Cardiac output increases by up to 45%, at first due primarily to elevated stroke volume, then mainly due to increased heart rate. Along with echocardiography, cardiac magnetic resonance imaging is safe for use in pregnancy. It may assess cardiac function more accurately than echocardiography, and may be indicated in specific clinical cases. Moreover, blood pressure decreases to a nadir in mid-pregnancy and rises to near preconception values postpartum. An appreciation of the vascular changes occurring in healthy pregnancy can aid in the prediction and diagnosis of pregnancy complications, such as preeclampsia and other hypertensive disorders of pregnancy, and inform treatment. In particular, noninvasive arterial stiffness/hemodynamics assessment provides unique clinical information beyond blood pressure and traditional maternal characteristics, and can signal a need for further testing, or be used in combination with other tests to predict or diagnose complications of pregnancy.
Skin programming of inflammatory responses to Staphylococcus aureus is compartmentalized according to epidermal keratinocyte differentiation status
Authors: Clayton Kalum , Holbrook Daniel J , Vallejo Andres , Porter Gemma , Sirvent Sofia , Davies James  , Pople Jenny , Lim Fei Ling , Christodoulides Myron , Polak Marta E , Ardern-Jones Michael R
Published: January 2023

Background

Acute cutaneous inflammation causes microbiome alterations as well as ultrastructural changes in epidermis stratification. However, the interactions between keratinocyte proliferation and differentiation status and the skin microbiome have not been fully explored.

Objectives

Hypothesizing that the skin microbiome contributes to regulation of keratinocyte differentiation and can modify antimicrobial responses, we examined the effect of exposure to commensal (Staphylococcus epidermidis, SE) or pathogenic (Staphylococcus aureus, SA) challenge on epidermal models.

Methods

Explant biopsies were taken to investigate species-specific antimicrobial effects of host factors. Further investigations were performed in reconstituted epidermal models by bulk transcriptomic analysis alongside secreted protein profiling. Single-cell RNA sequencing analysis was performed to explore the keratinocyte populations responsible for SA inflammation. A dataset of 6391 keratinocytes from control (2044 cells), SE challenge (2028 cells) and SA challenge (2319 cells) was generated from reconstituted epidermal models.

Results

Bacterial lawns of SA, not SE, were inhibited by human skin explant samples, and microarray analysis of three-dimensional epidermis models showed that host antimicrobial peptide expression was induced by SE but not SA. Protein analysis of bacterial cocultured models showed that SA exposure induced inflammatory mediator expression, indicating keratinocyte activation of other epidermal immune populations. Single-cell DropSeq analysis of unchallenged naive, SE-challenged and SA-challenged epidermis models was undertaken to distinguish cells from basal, spinous and granular layers, and to interrogate them in relation to model exposure. In contrast to SE, SA specifically induced a subpopulation of spinous cells that highly expressed transcripts related to epidermal inflammation and antimicrobial response. Furthermore, SA, but not SE, specifically induced a basal population that highly expressed interleukin-1 alarmins.

Conclusions

These findings suggest that SA-associated remodelling of the epidermis is compartmentalized to different keratinocyte populations. Elucidating the mechanisms regulating bacterial sensing-triggered inflammatory responses within tissues will enable further understanding of microbiome dysbiosis and inflammatory skin diseases, such as atopic eczema.
Validation of diagnostic criteria for atopic dermatitis and proposal of novel diagnostic criteria for adult and elderly Chinese populations: a multicentre, prospective, clinical setting-based study
Authors: Yue Wanbo , Cheng Daian , Sun Zhe , Shen Yihang , Wang Shangshang , Liu Xiumei , Pei Xiaoping , Deng Sisi , Pan Hongju , Liao Zhigang , Li Wei , Yao Xu , Liang Yunsheng , Song Zhiqiang , Yao Zhirong , Zhang Hui , Guo Yifeng
Published: January 2023

Background

A previous validation study showed a very low sensitivity and higher specificity associated with Hanifin and Rajka criteria (H&R) and the UK Working Party criteria (UKWP) in diagnosing AD vs. the Chinese criteria of atopic dermatitis (AD) for children (CCAD). However, their diagnostic efficacy in adult and elderly Chinese populations remains unknown.

Objectives

To validate the diagnostic efficacy of three sets of AD criteria in adult and elderly Chinese populations in a hospital setting.

Methods

A total of 1034 patients (aged 19–95 years) from five university hospital dermatological clinics were recruited. Medical history, dermatological examination, AD diagnosis and evaluation of AD severity were done by dermatologists. Each patient was investigated by two dermatologist panels, one to establish a clinical diagnosis, and the other to identify and record the major or minor signs of H&R criteria, UKWP criteria and CCAD. Taking clinical diagnosis as the reference, the diagnostic efficacy of three sets of diagnostic criteria was evaluated. The χ2 test or rank sum test were used for between-groups comparisons.

Results

CCAD had a higher sensitivity (84.0%), especially among mild and moderate cases of AD (72.7% and 90.3%, respectively), than the H&R (58.0%; P < 0.001) and UKWP criteria (56.0%; P < 0.001) in diagnosing AD. The specificity of CCAD (92.7%) was slightly lower than the H&R (97.3%; P < 0.001) or UKWP criteria (97.4%; P < 0.001). The CCAD had the highest Youden index (0.77), accuracy rate (0.90) and Kappa value (0.76) of the three sets of diagnostic criteria.

Conclusions

Consistent with results in a population of Chinese children, although the H&R and UKWP criteria had a high specificity for diagnosing AD, their low sensitivity limited their use in adult and elderly Chinese patients. Based on the high sensitivity and favourable diagnostic efficacy, the CCAD is proposed for AD diagnosis in adult and elderly Chinese populations, especially for cases of mild and moderate AD.
The changing face of anti-trust in the world of Big Tech: Collusion versus Monopolisation
Authors: Armoogum Prishnee , Davies Stephen , Mariuzzo Franco
Published: January 2023

This paper presents new evidence on two key developments in worldwide anti-trust in the last decade: (i) a downturn in the number of cartels detected by competition authorities and (ii) exponential growth in cases of monopolisation/abuse of dominance. Big Tech firms have been, undoubtedly, the main focus of the latter but almost totally absent in the former. These two developments offer perspectives on the description of Monopoly Capitalism as set out by Keith Cowling 40 years ago. Superficially at least, this seems to deny the prediction of ever-widening collusion, but, on the other hand, it resonates with the prediction of increasingly unassailable dominant firms. We suggest that the two trends can best be understood by the emergence of the Big Tech giants who have established dominance by exceeding tipping points in many markets. In turn, this leads to an alternative form of collusion—‘mutual forbearance’ in which firms back away from aggressive competition in the other giants’ areas of strength. Given this dominance, they do not need collusion—put simply, no sizeable rivals are remaining with whom they need to collude.
Lost in transition: a case report of very late palliation of a double outlet right ventricle
Authors: Ahmed Rizwan , Al Hashmi Haitham , Mora Bassem
Published: January 2023

Background

Children with congenital heart disease remain at risk of being lost to follow-up. They may present at a later stage with complications related to underlying condition that usually require in-depth phenotyping for optimal management. We describe an unusual case of a double outlet right ventricle (DORV) who presented three decades after initial intervention.

Case summary

A 32-year-old female sought attention for worsening breathlessness. Available data suggested that she had double outlet right ventricle (DORV) and underwent pulmonary artery (PA) banding in infancy. On examination, she was frail, clubbed and cyanosed with oxygen saturation reduced to 75% at rest. Blood tests demonstrated elevated haemoglobin (208 g/L) and thrombocytopenia (70 *109/L). Echocardiography was limited but demonstrated DORV with increased gradient across both outflow tracts. Cardiac magnetic resonance (CMR) imaging clearly demonstrated DORV with doubly committed interventricular communication, subvalvular aortic valve stenosis and supravalvular pulmonary stenosis due to a previous PA band. CMR was also remarkable for demonstrating severely impaired left ventricle and difficulty achieving optimal contrast between myocardium and blood pool on late enhancement images. She underwent relief of subaortic stenosis with immediate improvement in left ventricular (LV) function and PA plasty with application of a new PA band.

Conclusion

Lost to follow-up congenital patients present a particular challenge due to complications of chronic unusual physiology. This requires careful assessment by a multidisciplinary team to define optimal management strategy.
The potential of colchicine for lowering the risk of cardiovascular events in type 1 diabetes
Authors: Johansen Nicklas Järvelä , Knop Filip Krag
Published: January 2023

In type 1 diabetes, average life expectancy is reduced by ˃10 years as compared with outside of diabetes. Residual cardiovascular risk defines high cardiovascular event rate despite modern, guideline-recommended standard of care of established risk factors like hypertension, dyslipidaemia, and glycaemic control, and it adds importantly to these lost years of life in type 1 diabetes due to atherosclerotic cardiovascular diseases like myocardial infarction and ischaemic stroke. With a growing understanding of inflammation as an important driver of atherosclerotic cardiovascular disease, residual inflammatory risk is a novel and common risk factor and a promising target for lowering residual cardiovascular risk in type 1 diabetes. Interestingly, the inexpensive anti-inflammatory agent colchicine reduced the risk of major adverse cardiovascular events by 25% in cardiovascular outcome trials in the secondary prevention of atherosclerotic cardiovascular disease. Here, we summarize the role of inflammation as a driver of atherosclerosis and review current evidence linking inflammation and atherosclerotic cardiovascular disease in type 1 diabetes. Also, we provide an overview of the evidence base for targeting residual inflammatory risk with colchicine for lowering residual cardiovascular risk in type 1 diabetes.
Targeting endothelial tight junctions to predict and protect thoracic aortic aneurysm and dissection
Authors: Yang Xueyuan , Xu Chen , Yao Fang , Ding Qianhui , Liu Hao , Luo Congcong , Wang Daidai , Huang Jiaqi , Li Zhiqing , Shen Yicong , Yang Weijie , Li Zhuofan , Yu Fang , Fu Yi , Wang Li , Ma Qingbian , Zhu Junming , Xu Fujian , Cong Xin , Kong Wei
Published: January 2023

Aims

Whether changes in endothelial tight junctions (TJs) lead to the formation of thoracic aortic aneurysm and dissection (TAAD) and serve as an early indicator and therapeutic target remains elusive.

Methods and results

Single-cell RNA sequencing analysis showed aberrant endothelial TJ expressions in the thoracic aortas of patients with TAAD. In a β-aminopropionitrile (BAPN)-induced TAAD mouse model, endothelial TJ function was disrupted in the thoracic aortas at an early stage (5 and 10 days) as observed by a vascular permeability assay, while the intercellular distribution of crucial TJ components was significantly decreased by en face staining. For the non-invasive detection of endothelial TJ function, two dextrans of molecular weights 4 and 70 kDa were conjugated with the magnetic resonance imaging (MRI) contrast agent Gd-DOTA to synthesize FITC-dextran-DOTA-Gd and rhodamine B-dextran-DOTA-Gd. MRI images showed that both probes accumulated in the thoracic aortas of the BAPN-fed mice. Particularly, the mice with increased accumulated signals from 5 to 10 days developed TAAD at 14 days, whereas the mice with similar signals between the two time points did not. Furthermore, the protease-activated receptor 2 inhibitor AT-1001, which seals TJs, alleviated the BAPN-induced impairment of endothelial TJ function and expression and subsequently reduced TAAD incidence. Notably, endothelial-targeted ZO-1 conditional knockout increased TAAD incidence. Mechanistically, vascular inflammation and edema were observed in the thoracic aortas of the BAPN-fed mice, whereas these phenomena were attenuated by AT-1001.

Conclusion

The disruption of endothelial TJ function is an early event prior to TAAD formation, herein serving as a potential indicator and a promising target for TAAD.
Unipolar voltage mapping in right ventricular cardiomyopathy: pitfalls, solutions and advantages
Authors: Hoogendoorn Jarieke C , Venlet Jeroen , de Riva Marta , Wijnmaalen Adrianus P , Piers Sebastiaan R D , Zeppenfeld Katja
Published: January 2023

Aims

Endocardial unipolar and bipolar voltage mapping (UVM/BVM) of the right ventricle (RV) are used for transmural substrate delineation. However, far-field electrograms (EGMs) and EGM changes due to injury current may influence automatically generated UVM. Epicardial BVM is considered less accurate due to the impact of fat thickness (FT). Data on epicardial UVM are sparse. The aim of the study is two-fold: to assess the influence of the manually corrected window-of-interest on UVM and the potential role of epicardial UVM in RV cardiomyopathies.

Methods and results

Consecutive patients who underwent endo-epicardial RV mapping with computed-tomography (CT) integration were included. Mapping points were superimposed on short-axis CT slices and correlated with local FT. All points were manually re-analysed and the window-of-interest was adjusted to correct for false high unipolar voltage (UV). For opposite endo-epicardial point-pairs, endo-epicardial bipolar voltage (BV) and UV were correlated for different FT categories. A total of 3791 point-pairs of 33 patients were analysed. In 69% of endocardial points and 63% of epicardial points, the window-of-interest needed to be adjusted due to the inclusion of far-field EGMs, injury current components, or RV-pacing artifacts. The Pearson correlation between corrected endo-epicardial BV and UV was lower for point-pairs with greater FT; however, this correlation was much stronger and less influenced by fat for UV.

Conclusion

At the majority of mapping sites, the window-of-interest needs to be manually adjusted for correct UVM. Unadjusted UVM underestimates low UV regions. Unipolar voltage seems to be less influenced by epicardial fat, suggesting a promising role for UVM in epicardial substrate delineation.
Syncope in ICD recipients: a single centre experience
Authors: Khan Parisha , Selvarajah Karshana , Gohel Sheena , Sidhu Baldeep S , Cannatà Antonio , Bromage Daniel I , McDonagh Theresa , Murgatroyd Francis , Scott Paul A
Published: January 2023

Aims

There is little evidence of the impact of syncope in implantable cardioverter-defibrillator (ICD) patients in routine community hospital care. This single-centre retrospective study sought to evaluate the incidence and prognostic significance of syncope in consecutive ICD patients.

Methods and results

Data were collected on consecutive patients undergoing first ICD implantation between January 2009 and December 2019. The primary endpoints were the first occurrence of all-cause syncope, all-cause mortality, and all-cause hospitalization. Multivariate Cox proportional hazard models were used to identify risk factors associated with syncope and to analyse the subsequent risk of mortality and hospitalization. 1003 patients (58% primary prevention) were included in the final analysis. During a mean follow-up of 1519 ± 1055 days, 106 (10.6%) experienced syncope, 304 died (30.3%), and 477 (47.5%) were hospitalized for any cause. In an analysis adjusted for baseline variables, the first occurrence of syncope was associated with a significantly increased risk of mortality (HR 2.82, P < 0.001) and the first occurrence of hospitalization (HR 2.46, P = 0.002).

Conclusion

Syncope in ICD recipients is common and associated with a poor prognosis irrespective of baseline variables and ICD programming. The occurrence of syncope is associated with a significant increase in the risk of mortality and hospitalization.
pCEC-red: a new vector for easier and faster CRISPR-Cas9 genome editing in Saccharomyces cerevisiae
Authors: Maestroni Letizia , Butti Pietro , Senatore Vittorio Giorgio , Branduardi Paola
Published: January 2023

CRISPR-Cas9 technology is widely used for precise and specific editing of Saccharomyces cerevisiae genome to obtain marker-free engineered hosts. Targeted double-strand breaks are controlled by a guide RNA (gRNA), a chimeric RNA containing a structural segment for Cas9 binding and a 20-mer guide sequence that hybridises to the genomic DNA target. Introducing the 20-mer guide sequence into gRNA expression vectors often requires complex, time-consuming, and/or expensive cloning procedures. We present a new plasmid for CRISPR-Cas9 genome editing in S. cerevisiae, pCEC-red. This tool allows to (i) transform yeast with both Cas9 and gRNA expression cassettes in a single plasmid and (ii) insert the 20-mer sequence in the plasmid with high efficiency, thanks to Golden Gate Assembly and (iii) a red chromoprotein-based screening to speed up the selection of correct plasmids. We tested genome-editing efficiency of pCEC-red by targeting the ADE2 gene. We chose three different 20-mer targets and designed two types of repair fragments to test pCEC-red for precision editing and for large DNA region replacement procedures. We obtained high efficiencies (∼90%) for both engineering procedures, suggesting that the pCEC system can be used for fast and reliable marker-free genome editing.
Risk of occupational infection in forensic workers: a review
Authors: Dufayet Laurène , Langrand Jerome , Ludes Bertrand
Published: January 2023

The occupational risk of infection in forensic workers is a cause for concern, furthermore in the current context of the coronavirus disease-19 (COVID-19) pandemic. In order to characterize this risk, we performed an extended review of the literature on occupational infections occurring in forensic workers. Seventeen articles were included. Direct contamination by aerosolization was the main mode of transmission reported, with 17 cases of tuberculosis. Indirect contamination was described as the mode of transmission in 10 cases (five cases of blastomycosis, two cases of tuberculosis, two Streptococcus pyogenes, and one case of human immunodeficiency virus). In all the other included cases, the mode of transmission was unknown. For two of them, the information provided was sufficient to link them to occupational exposure (one case of toxoplasmosis, one case of tuberculosis). For the remaining 10 cases, the link was uncertain (six cases of tuberculosis, three of hepatitis B, and one of COVID-19). Even if there is probably significant under-declaration, the number of infections linked to an occupational risk in forensic workers is not alarming, thanks to effective preventive measures.
Joint inversion of GNSS and GRACE/GFO data for terrestrial water storage changes in the Yangtze River Basin
Authors: Li Xianpao , Zhong Bo , Li Jiancheng , Liu Renli
Published: January 2023

Satellite geodetic technologies, such as the Global Navigation Satellite System (GNSS), Gravity Recovery and Climate Experiment (GRACE) and GRACE Follow-On (GFO), have complementary advantages in inferring terrestrial water storage (TWS) changes at regional and basin scales. We introduced a joint inversion strategy to infer TWS changes using GNSS- and GRACE/GFO-derived vertical displacements based on Green's function theory in the Yangtze River Basin (YRB) from January 2011 to December 2020. Additionally, we investigated the performance of variance component estimation (VCE) and Akaike's Bayesian Information Criterion (ABIC) to determine the optimal relative weights of different observation data. The performance of our joint inversion strategy was verified through a closed-loop simulation and multi-source hydrometeorological data [i.e. the time derivative of TWS changes (${\rm d}S/{\rm d}t$) from precipitation (P), evapotranspiration (ET) and run-off (R) based on the water balance equation, called P-ET-R]. The closed-loop simulation shows that the TWS changes from joint inversion have better consistencies with the synthetic signals than those of GNSS- and GRACE-only estimates, and the corresponding root mean square error (RMSE) decreased 1.43−6.28 mm and correlation coefficient (CC) increased 3−10 per cent. The ABIC was more suitable for the joint inversion of measured GRACE/GFO and GNSS data for TWS changes in the YRB. Analysis from the measured data shows that the spatial patterns and seasonal characteristics in TWS changes derived from GNSS, GRACE/GFO and their joint inversion are in good agreement in the YRB. The contribution of GNSS observations to the joint inversion in the upstream of the YRB is greater than that of GRACE/GFO due to the relatively densely distributed GNSS stations, but the opposite is true in the downstream. Furthermore, the joint inversion results have better agreements with P and P-ET-R compared to GNSS- and GRACE/GFO-only estimates in the upstream, and the corresponding CCs increased 5−7 per cent (for P) and 2−5 per cent (for P-ET-R), respectively, which further demonstrates the effectiveness of our joint inversion strategy. Our estimation strategy provides a new insight for joint inversion of GNSS and GRACE/GFO data to obtain more reliable TWS changes.
Multiple streams approach to tobacco control policymaking in the capital city of the world’s largest tobacco consuming country
Authors: Zheng Yunting , Shi Yuhui , Ji Ying , Chang Chun
Published: January 2023

The aim of this study was to analyse the policymaking process of Beijing tobacco control regulations based on a multiple streams framework to provide a reference for other cities, at the national level and for the international community to promote the development of tobacco control policy. Twenty-one documents related to tobacco control in Beijing were collected, nine informants were interviewed and the interview data were analysed by a thematic framework method. It was found that indicators, feedback and a focus event in the problem stream drew the attention of policymakers and the society for tobacco control. In 2011, Ying Songnian, a representative of the Beijing Municipal People’s Congress, put forward tobacco control legislation, which was just in time for the legislative reform of the Congress. The proposal was studied by the Congress, and a strategy of ‘social co-governance’ was founded. In the political stream, the government actively promoted tobacco control and social organizations extensively participated in it. In 2013, the General Office of the Central Committee of the Chinese Communist Party and the General Office of the State Council issued a notice on matters related to leading cadres taking the lead in banning smoking in public places, which opened a policy window for decision-makers who were hesitatant. The issue of tobacco control was successfully put on the policy agenda and contributed to the introduction of the Beijing Municipal Regulations on Smoking Control. Development of the factors in problem stream, policy stream and political stream promoted the setting up of the tobacco control policy agenda in Beijing. It is suggested that more cities should learn from the experience of Beijing, seize the opportunity of the ideological change of the ruling party, actively identify the problems, mobilize and advocate for representatives and introduce the concept of ‘social co-governance’ to promote tobacco control legislation.
Impact of Baseline Disease Activity and Trial Duration on Efficacy of Biologics in Active Crohn’s Disease: Meta-analysis
Authors: Barberio Brigida , Gracie David J , Black Christopher J , Ford Alexander C
Published: January 2023

Background

Timings of assessment of efficacy and criteria used to define Crohn’s disease (CD) activity at baseline may affect therapeutic gain of active drug over placebo in induction of remission trials in CD, but these issues have not been assessed systematically. We examined these issues in a meta-analysis.

Methods

We searched the literature to June 2022 for randomized controlled trials of biologics vs placebo in active CD. We extracted clinical remission and response rates according to criteria used to define CD activity and time point of assessment, pooling them in a meta-analysis for all patients according to previous biologic exposure. We calculated the number needed to treat (NNT), with a 95% confidence interval (CI) to assess therapeutic gain of active drug over placebo according to these characteristics of trial design.

Results

We identified 20 induction of remission trials (6754 patients). Rates of clinical remission were highest (42.6% with active drug vs 21.0% with placebo) and NNT lowest (5; 95% CI, 3-7.5) in trials using clinical and endoscopic activity to define active CD. Rates of remission were lower (26.5% with active drug, vs 18.6% with placebo) and NNT highest (12; 95% CI, 6-61) in trials using clinical activity alone. Results were similar according to previous biologic exposure. Time point of assessment seemed to have less of an effect, although the NNT was lowest in trials assessing remission rates at 9 to 12 weeks (NNT = 5.5; 95% CI, 4-8). Again, results were similar according to previous biologic exposure.

Conclusions

Both the criteria used to define CD activity at study entry and the time point used to confirm efficacy may be important in maximizing therapeutic gain of active drug over placebo.
Appropriate Preconception Corticosteroid-Free Remission Period in Pregnant Women With Ulcerative Colitis
Authors: Ikeda Aya , Kunisaki Reiko , Aoki Shigeru , Yaguchi Katsuki , Madarame Akira , Nishio Masafumi , Ogashiwa Tsuyoshi , Nakamori Yoshinori , Kimura Hideaki , Suzuki Ryoichi , Saigusa Yusuke , Maeda Shin
Published: January 2023

Background

In women with inflammatory bowel disease, at least 3 months of preconception corticosteroid-free remission (CFREM) is recommended by experts in current consensus statements. However, data are lacking on the appropriate preconception remission period. We investigated the appropriate preconception CFREM period in women with ulcerative colitis to reduce maternal disease activity and adverse pregnancy outcomes (ie, preterm birth, low birth weight, and small for gestational age).

Methods

We retrospectively examined 141 pregnancies in women with ulcerative colitis at 2 institutions. We categorized the patients into 3 subgroups by their preconception CFREM period (≥3 months, >0 to <3 months, and non-CFREM). We also investigated disease activity during pregnancy and postpartum and adverse pregnancy outcomes in each group.

Results

During pregnancy, the rate of active disease was significantly lower in the ≥3 months and >0 to <3 months CFREM groups compared with that in the non-CFREM group (P < .001 and P = .0257, respectively). Postpartum, the rate of active disease was significantly lower in the ≥3 months CFREM group compared with that in the non-CFREM group (P = .0087). The preconception CFREM period of ≥3 months was an independent inhibitory factor for active disease during pregnancy and postpartum (adjusted odds ratio, 0.15; P = .0035; and adjusted odds ratio, 0.33; P = .027, respectively). Adverse pregnancy outcomes were less common in the >3 months CFREM group compared with those in the other groups, but this difference was not significant.

Conclusions

A preconception CFREM period of more than 3 months may be appropriate for better maternal and adverse pregnancy outcomes, as recommended in consensus statements.
Green innovation strategies and firms’ internationalization
Authors: Martínez-Ros Ester , Merino Fernando
Published: January 2023

It is well established in the literature that a firm’s innovation can promote its exports through demand-pull and/or regulatory channels. However, there is a lack of knowledge on whether green innovation strategies affect a firm’s international expansion. In this study, we depart from the existing literature by considering the introduction of environmental innovation as an antecedent factor that creates incentives for a firm to enter new markets and to be persistent in export activities. In particular, we investigate whether strategies aimed at reducing energy or water consumption and taking care of the environment have an impact on the probability of being an exporter and on the probability of continuing to export. The results, for a panel data of Spanish firms, confirm the existence of a premium on the probability of entering and remaining in international markets. Commitment to green innovation strategies, such as reductions in energy and water consumption or the minimization of environmental impact, generates a premium for companies that internalize their activities.
Upregulation ofPTPRCand Interferon Response Pathways in HIV-1 Seroconverters Prior to Infection
Authors: Li Yunqi , Lefebvre Francois , Nakku-Joloba Edith , Ronald Allan , Gray Glenda , de Bruyn Guy , Kiarie James , Celum Connie , Cameron Mark J , Lingappa Jairam R , Mackelprang Romel D
Published: January 2023

Human immunodeficiency virus 1 (HIV-1) exposed seronegative (HESN) individuals may have unique characteristics that alter susceptibility to HIV-1 infection. However, identifying truly exposed HESN is challenging. We utilized stored data and biospecimens from HIV-1 serodifferent couple cohorts, in which couples’ HIV-1 exposures were quantified based on unprotected sex frequency and viral load of the partner with HIV-1. We compared peripheral blood gene expression between 15 HESN and 18 seroconverters prior to infection. We found PTPRC (encoding CD45 antigen) and interferon-response pathways had significantly higher expression among individuals who went on to become seropositive and thus may be a signature for increased acquisition risk.
Plasma concentration of orally administered amoxicillin and clindamycin in patients receiving haemodialysis
Authors: Solli Camilla N , Bock Magnus , Kaur Kamal P , Kristensen Jonas H , Greibe Eva , Hansen Henrik P , Boesby Lene , Borg Rikke , Chaudry Mavish , Hoffmann-Lücke Elke , Moser Claus , Falkentoft Alexander C , Fosbøl Emil , Østergaard Lauge , Torp-Pedersen Christian , Bundgaard Henning , Iversen Kasper , Bruun Niels E
Published: January 2023

Objectives

In the randomized controlled trial PANTHEM, the prophylactic effect of oral amoxicillin or clindamycin is investigated in patients receiving chronic haemodialysis (HD). However, data on plasma concentrations of these antibiotics during HD are sparse. This study aims to determine if the plasma concentration of amoxicillin and clindamycin is sufficient during HD after oral administration of amoxicillin and clindamycin at three different time intervals prior to the HD procedure.

Methods

Adult patients receiving chronic HD were investigated twice with an interval of at least 7 days starting with either a tablet of 500/125 mg amoxicillin/clavulanic acid or a tablet of 600 mg clindamycin. Patients were randomized to take the antibiotics either 30, 60 or 120 min prior to the HD procedure. Plasma antibiotic concentrations were measured at start, midway and at the end of HD. A lower threshold was set at 2.0 mg/L for amoxicillin and at 1.0 mg/L for clindamycin. In addition, a population pharmacokinetic (PK) analysis was performed, assessing PTA.

Results

In the amoxicillin cohort (n = 37), 84% of patients and 95% of all plasma amoxicillin concentrations were above or at the threshold throughout the dialysis procedure. In the clindamycin cohort (n = 33), all concentrations were above the threshold throughout the dialysis procedure. Further, in all patients, the mean plasma concentration of both amoxicillin and clindamycin across the HD period was well above the threshold. Finally, the PK model predicted a high PTA in the majority of patients.

Discussion

In patients on chronic HD, oral administration of amoxicillin/clavulanic acid (500/125 mg) or clindamycin (600 mg) within 30–120 min prior to HD leads to a sufficient prophylactic plasma concentration across the HD period.
Survival of Salmonella spp. and Pathogenic Escherichia coli in Food Matrixes and Its Relevance in the Development of Proficiency Testing Samples
Authors: Ashish Singh Kumud , Nair Santhini S , Rai Rohit
Published: January 2023

Monitoring of food products by government agencies for their compliance to regulatory limits is an essential step in controlling foodborne outbreaks. For monitoring purposes, an extensive setup of the surveillance system is used, which involves ISO 17025:2017 accredited laboratories for food testing. Participation in proficiency testing (PT) programs is a requirement of ISO 17025:2017, which ensures data accuracy and analyst competency. Participation in PT schemes is costly for laboratories in developing countries as most of the commercial suppliers are situated in the United States and Europe. The literature or data available on creation of microbiological proficiency testing is scanty as much of the data available with commercial suppliers are trade secrets, and there is only 0.06% of research articles available in the Scopus database on the topic. In this review article, an attempt is made to understand the factors impacting the survival of two important foodborne pathogens, i.e., Escherichia coli and Salmonella spp., by extracting information available from growth studies and root-cause analysis of various food safety incidents and recalls. Utilization of this information in the development of PT samples is discussed in this review article along with a focus on the availability of PT samples and associated ISO standards to formulate homogeneous and stable PT samples. This review article elaborates on the focus areas that can be considered by PT providers (PTP)—for example, initial inoculum level and preparation, strain type, microbial growth phase, the impact of different types of food matrixes including low-moisture food, antimicrobial components, pH, presence of competitor microbes, and environmental conditions involving storage temperature, time, and relative humidity. These focus areas can be used to successfully create PT samples by PTP in developing countries.
Undergraduate student attitudes to current poultry industry issues over four semesters: surveying an introductory poultry science course
Authors: Meyer Meaghan M , Bobeck Elizabeth A
Published: January 2023

Individual background and demographics affect student perceptions of animal production. Understanding how science-based education alters these opinions is a critical aspect of improving university instruction as well as increasing consumer engagement in the poultry industry. The study objectives were to quantify the effects of student background, career interests, and science-based instruction on opinions regarding current issues in the poultry industry. Undergraduate students enrolled in a one semester poultry science course at Iowa State University between 2018 and 2021 were anonymously surveyed at the start and end of the semester as part of a 4-yr study. Students who opted to take the survey answered three demographic questions indicating their 1) livestock experience, 2) sex, and 3) career goals. The body of the survey consisted of 16 “poultry issue statements” where students were directed to mark a vertical dash on a 130 mm horizontal line indicating their level of agreement with each statement. Post-survey collection, the line was separated into 5 sections for discussion: responses within 0%–20% indicated strongly disagree, 21%–40% disagree, 41%–60% neutral, 61%–80% agree, and 81%–100% indicated strongly agree. Responses were analyzed using Proc Mixed in SAS Version 9.4 with a Tukey–Kramer adjustment for all pairwise comparisons using main effects including demographic categories, education (pre- or post-instruction), and year the survey was taken. Responses to various issue statements were affected by students’ livestock experience (P < 0.05; 6 out of 16 statements affected), sex (P < 0.05; 5 out of 16 statements), and ultimate career goals (P < 0.05; 4 out of 16 statements). Pre- vs. post-education responses differed significantly in 6 out of 16 statements (P < 0.05), and in 2 out of 16 poultry issue statements, the year of instruction affected student response (P < 0.05). These data indicate that individual student background, sex, and differing career interests impact opinions of current topics in the broiler and layer industries. Further, science-based education as well as the year the course was taken over consecutive semesters significantly altered student opinions.
Effects of replacing corn silage with alfalfa haylage in growing beef cattle diets on performance during the growing and finishing period
Authors: Tarnonsky Federico , Hochmuth Katherine , DiCostanzo Alfredo , DiLorenzo Nicolas
Published: January 2023

Corn silage is the predominant mechanically harvested forage source for feedlot cattle production in the United States because of high yield. Alternatively, because of multiple cuttings per year and lower annual cost, the use of alfalfa or other forages, may increase opportunities for manure spreading, perennial soil cover, pollinator habitat, and greater carbon sequestration. The objective of this trial was to determine the feeding value of alfalfa haylage when replacing corn silage in growing cattle diets. One-hundred-sixty-five Angus crossbred steers [326 ± 51 kg of body weight (BW)] were blocked by initial BW and randomly assigned to one of 28 pens at the University of Minnesota feedlot. Pens were randomly assigned to dietary growing treatments. The control diet was comprised of (DM basis) 50% corn silage, 19.25% rolled corn grain, 19.25% high moisture corn, 7% dried distillers grains plus solubles, and 4.5% liquid supplement (corn silage control, CS Control). For alfalfa haylage (AH) diets, AH substituted corn silage at 33% (AH 33), 66% (AH 66), or 100% (AH 100). Growth performance measurements [dry matter intake (DMI), average daily gain (ADG) and gain to feed (G:F) ratio] were assessed for 42 to 70 d depending on BW block. Afterwards, steers were fed a common finishing diet until harvested. There was a linear increase in DMI (P < 0.01) with increasing AH inclusion. Replacing CS with AH linearly decreased (P ≤ 0.05) ADG and G:F. No differences (P ≥ 0.10) were observed in finishing performance or carcass traits. Results from this study demonstrated that greater substitution of corn silage with alfalfa haylage in growing diets resulted in greater intake but reduced rate of gain and gain:feed. Despite slower rate of gain, cattle fed alfalfa haylage at increasing proportions during the growing period were able to compensate in BW gains during the finishing period and reached harvest weight and backfat thickness at similar days on feed than those fed corn silage. Based on these results the energy value of corn silage and alfalfa haylage were 3.05 and 2.39 Mcal ME/kg of DM, respectively, when included at 50% of the diet DM.
Taraxasterol alleviates fatty acid-induced lipid deposition in calf hepatocytes by decreasing ROS production and endoplasmic reticulum stress
Authors: Li Ming , He Yuxin , Zhang Wei , Yin Yufeng , Jiang Qianming , Loor Juan J , Wang Jingjing , Wen Jianan , Yang Wei , Xu Chuang , Zhang Bingbing
Published: January 2023

Increased concentrations of free fatty acids (FFAs) induce reactive oxygen species (ROSs) generation and endoplasmic reticulum (ER) stress, thus, increasing the risk of fatty liver in dairy cows during the periparturient period. In non-ruminants, Taraxasterol (Tara; a pentacyclic triterpenoid found in medicinal plants) plays an important role in anti-inflammatory and antioxidant reactions. Whether Tara can alleviate or prevent fatty liver in ruminants is unknown. We addressed whether Tara supply could dampen lipid accumulation, ROSs production, and ER stress caused by FFAs in calf hepatocytes. Primary calf hepatocytes were isolated from five healthy calves (1 d old, female, 30–40 kg, fasting, rectal temperature 38.7–39.7 °C). In the first experiment, hepatocytes were incubated with various concentrations of Tara (2.5, 5, and 10 μg/mL) for 12 h prior to the 1.2-mM FFAs challenge. Results indicated that the level of ROSs was lowest with 5 μg/mL Tara. Thus, to further characterize the molecular mechanisms whereby Tara protects from FFAs-induced lipid deposition in calf hepatocytes, we performed incubations with 5 μg/mL Tara for 12 h prior to a 1.2-mM FFAs challenge for an additional 12 h. Results indicated that 1.2-mM FFAs challenge increased mitochondrial membrane potential (MMP), enhanced expression of proteins and mRNA associated with ER stress (PERK, IRE1, GRP78, ATF6, and CHOP) and fatty acid synthesis (FASN, ACC1, and SREBP-1c), and ultimately led to increased lipid droplet synthesis. In contrast, Tara treatment alleviated these negative effects after 1.2-mM FFAs challenge. To determine whether Tara protects against FFAs-induced lipid droplet synthesis by alleviating oxidative stress, hepatocytes were treated with 5 μg/mL Tara for 22 h prior to H2O2 (440 μM) challenge for 2 h. Compared with H2O2 treatment alone, results revealed a marked decrease in ROSs, MMP, and protein abundance of ER stress (GRP78, ATF6, and CHOP) and lipid droplet synthesis in response to Tara prior to H2O2 challenge. Data suggested that the increase in mitochondrial ROSs production contributes to lipid accumulation in calf hepatocytes. Collectively, our in vitro data indicate that Tara alleviates fatty acid-induced lipid deposition. Further research is warranted to ascertain that Tara can be helpful in the therapeutic management of early lactating cows to control or alleviate excessive hepatic lipid deposition.
Integrating Palliative Care into Burn Care: A Retrospective Application of Criteria for Appropriate Referral
Authors: Grossoehme Daniel H , Bish Brianna M , Lou Richard , Khandelwal Anjay , Brown Miraides , Brown Carrie , Teo Esther , Friebert Sarah
Published: January 2023

Burn injuries often require complex interdisciplinary care. Despite potential benefits, consultation with specialist palliative care typically occurs in 2% of burn admissions, due largely to lack of availability and misconceptions concerning palliative care. Criteria to guide consultation with specialist palliative care have been developed at this institution. The purpose of this study was to quantify the sensitivity and specificity of these criteria, and then optimize them. Retrospective examination of 388 admissions over 15 months at this adult and pediatric Burn Center were conducted. Chi-square or Fisher’s exact test for categorical variables and Wilcoxon Rank Sum test for continuous variables were used to test for between-group differences in demographic and clinical characteristics. Logistic regression model was built to determine modified Baux cut-off score using Youden index (J) value. Criteria were sensitive (0.96) and specific (0.90), though with a low positive predictive value (0.43). Persons with lengths of stay greater than one week, advanced directives, Allow Natural Death orders, and/or deterioration events were significantly more likely to have had palliative care consulted. There were significant between-group differences (with/without palliative care consultation) based on burn surface area, modified Baux score, and mortality risk. Potential criteria revisions are proposed. Prospective adherence to revised criteria could provide useful guidance to providers and benefit to patients and families.