bluuebunny/crossref_metadata_2025 huggingface dataset:
Review of the genus Miscophus Jurine, 1807 (Hymenoptera: Crabronidae: Crabroninae) of the Indian subcontinent with descriptions of two new species from southern India
Authors: KUMAR P. GIRISH , MAWADDA N.V. AYISHA , TERINE JOSHUA B. , AMAL S.
Published: March 2024

The digger wasp genus Miscophus Jurine, 1807 from the Indian subcontinent is reviewed with descriptions of two new species from India, Miscophus kaleshi Girish Kumar and Miscophus prasanthi Girish Kumar. A key to Miscophus of the Indian subcontinent is provided.
In vivo Antihyperlipidemic and Antioxidant Effect of Oil Extracted from Sardinella maderensis (Lowe, 1838) on Strain Wistar Rats
Authors: Koule Jules Christophe Manz , Tuem Régine Somon , Ngangue Roland Jethro Ekwalla Misse , Dongmo Fabrice Fabien Dongho , Nchoutpouen Merlin Ngafon , Nsoga Jean Valery François , Ndomou Mathieu , Gouado Inocent
Published: March 2024

Cardiovascular diseases (CVD) are one of the leading causes of death and disability, the main cause of which is hyperlipidemia. This work aimed to evaluate the antihyperlipidemic and antioxidant in vivo potential of oil extracted from Sardinella maderensis on rats. The oil was extracted according to the method of Bligh and Dyer and underwent chemical analysis prior to in vivo assays. After studying in vivo the acute toxicity of S. maderensis oil, their antihyperlipidemic was assessed. Twenty-four male Wistar adult rats were randomly divided into four groups of 6 rats each. During a three-week experiment, group 1 was fed with standard laboratory diet (SLD); group 2 received SLD supplemented with boiled egg yolk (5 g/day/rat); group 3 was fed with SLD supplemented with S. maderensis oil (1 g/day/kg of body mass) and group 4 was fed with SLD supplemented with boiled egg yolk and S. maderensis oil. Liver, lung, kidney, adipose tissues and heart were later removed, weighted and analyzed. Some blood biochemical and oxidative stress parameters were also measured. Results showed that S. maderensis oil was siccative, good quality with a lethal dose greater than 5000 mg/Kg of CP and no signs of toxicity were observed. Hyperlipidemic diet increased significantly (p ˂ 0.05) lipid profil, glycemia, uremia, activity of transaminase and γGT, oxidative stress in group 2 compared to other groups. Supplementation with S. maderensis oil significantly (p˂0.05) reduced Lee's index, weight gain and BMI by 8.12%, 26.33% and 19.11% respectively in group 4 compared with group 2. Supplementation with S. maderensis oil decreased significantly (p ˂ 0.05) total-cholesterol, LDL-cholesterol, triglyceride, glycemia, proteinemia and increased levels of HDL-cholesterol in group 4 compared to group 2. Supplementation with S. maderensis oil significantly (p < 0.05) reduced TBARS levels by 54.36% and significantly (p < 0.05) increased GSH levels, SOD, CAT and PON-1 activity by 64.90%, 20.76%, 48.70% and 7.47 respectively in group 4 compared to group 2. This study shows that S. maderensis oil can be used in prevention of hyperlipidemia.
Jammu and Kashmir on the Path of Empowering Grassroots Democracy
Authors: Waza Farooq Ahmad , Chathukulam Jos
Published: March 2024

The de-operationalisation of Article 370 has given a new lease of life to grassroots democracy in the erstwhile State of Jammu and Kashmir. The Union government has made it clear that the main priority is to strengthen grassroots democracy there. At present, the erstwhile State which was once battered by militancy, insurgency as well economic and political instability has embarked on the path of democracy. Though there have been criticisms that the eagerness and interest taken by the Union government to foster local democracy in Kashmir is an attempt to whitewash the absence of democratically elected State government, the recent developments in the erstwhile State including the completion of delimitation exercise at a quick pace to form democratically elected governments in the union territories of Jammu and Kashmir implies that there is some hope at end of the tunnel.
Creating a Space to Feel: An Integrative Therapy Approach with an Adolescent Diagnosed with Dissociative Motor Disorder
Authors: Wahi Arushee , Saxena Osheen , Sharma Eesha , Kishore M. Thomas
Published: March 2024

The dissociative motor disorder is one of the most commonly diagnosed forms of dissociative disorder in the Indian population, especially among adolescents. However, there is a dearth of literature on effective psychotherapy practices in the Indian setting. This article details the unique process of employing an integrative psychotherapy approach with a 16-year-old adolescent girl diagnosed with dissociative motor disorder who presented with complaints of headache, deviation of mouth, multiple somatic complaints, inability to move her legs, episodes of loss of consciousness with jerking of upper limbs and a non-pervasive low mood. A detailed evaluation highlighted significant disruptions in attachment with caregivers, complicated grief, significant anger towards her family which did not find a space for expression, unhealthy coping mechanisms in the background of a slow-to-warm-up temperament and sociocultural stressors. The case formulation drew elements from the psychodynamic perspective, family systems approach, biosocial theory and the cognitive behavioural perspective. The intervention from the first session until termination is delineated in different phases which outline the significant themes that emerged along with the techniques used, challenges faced and therapist reflections. The role of the therapeutic alliance, working through transference, systemic work, primary and secondary gains and the importance of defences along with the aftermath of losing them becomes especially salient as the psychotherapy progresses in this case. The implications of the therapy process that can hold relevance to the management of dissociation disorder in the clinical setting are also discussed.
The Prevalence and Correlates of Internet Addiction in India as Assessed by Young’s Internet Addiction Test: A Systematic Review and Meta-analysis
Authors: Tadpatrikar Ashwini , Sharma Manoj Kumar , Amudhan Senthil , Desai Geetha
Published: March 2024

Background:

Earlier research on internet addiction (IA) in the adult population in India has concentrated on isolated prevalence surveys conducted nationwide. A systematic grasp of pooled prevalence necessitates a meta-level evaluation of all prior literature. This study aims to investigate the prevalence of IA and its correlates among adults in India.

Methods:

We searched EBSCOhost, PubMed, PsycInfo, PsycArticles, and ProQuest for the studies published from 1990 to 2022 and included studies using Young’s Internet Addiction Test criteria (age range 18–40 years). The authors obtained a total of 358 non-duplicate records after a thorough search, and they found 10 pertinent epidemiological studies involving 12,286 adults.

Results:

The pooled prevalence for mild, moderate, severe, and any level of IA was 36.33% (95% confidence interval [CI]: 30.72%–42.13%), 18.04% (95% CI: 13.49%–23.08%), 0.88% (95% CI:0.50%–1.36%), and 58.34% (95% CI:49.34%–67.07%), respectively. The review of correlates suggested psychosocial factors like male gender, single-parent families, co-morbidities like depression, stress, anxiety, interpersonal issues, andmediating factors like loneliness.

Conclusions:

Based on the synthesis of findings, this review underlines the common factors of IA and emphasizes the significance of psycho-social factors in IA; thus, to promote healthy use of technology and to manage IA, a multi-modal psychotherapeutic intervention module for mental health practitioners needs to be developed in line with this review.
Early Career Nurses’ Experiences of Engaging in a Leadership Role in Hospital Settings
Authors: Ting Justine Jeanelle , Babenko-Mould Yolanda , Garnett Anna
Published: March 2024

Background

Early career nurses (ECNs) can be expected to assume shift charge nurse leadership roles quickly upon entering practice. Since the emergence of the COVID-19 pandemic, junior nurses may find their leadership capabilities tested further as the challenges of leadership are made increasingly complex in the context of an infectious disease outbreak.

Purpose

The purpose of this qualitative study was to explore early career registered nurses’ (RNs) experiences of engaging in shift charge nurse roles in hospital settings.

Methods

This study used an interpretive descriptive (ID) approach. Semi-structured, in-depth interviews were conducted with 14 RNs across Ontario, who had up to three years of experience and who had engaged in a shift charge nurse role in a hospital setting. Recruitment and data collection took place from January to May 2021 during the COVID-19 pandemic. Interviews were recorded, transcribed, and analyzed following the principles of content analysis.

Results

Content analysis of the 14 participant interviews revealed four main themes: nominated and necessitated into leadership, diverse and demanding responsibilities, factors that help and hinder, and leadership as an impactful experience.

Conclusions

Study findings provide insights into potential strategies to support ECNs in shift charge nurse roles, during the remaining course of the COVID-19 pandemic and beyond. Greater support for nurses who engage in these roles may be achieved by promoting collaborative unit and organizational cultures, prioritizing leadership training programs, and strengthening policies to provide greater clarity regarding charge nurse role responsibilities.
Uncovering Determinants of Entrepreneurial Behaviour in a Transitioning Economy: A Fuzzy-set Qualitative Comparative Analysis
Authors: Al-Silefanee Rebean , Bosma Niels
Published: March 2024

The purpose of this research article is to explore the determinants of entrepreneurial behaviour among university students in the Kurdistan Region of Iraq. The study uses a novel approach of fuzzy-set qualitative comparative analysis (fsQCA) to depart from traditional assumptions that causal mechanisms apply equally to all individuals within a group. The study examines how shared characteristics may influence entrepreneurial behaviour. The study contributes to the theory of planned behaviour (TPB) literature by identifying different configurations of factors that lead to entrepreneurial behaviour. The study finds that these configurations vary depending on the student’s gender, personal valuation of entrepreneurship, self-evaluation, and perceived support from their environment. These findings suggest that the context in which students are situated plays a crucial role in shaping their entrepreneurial behaviour, highlighting the importance of considering within-sample variations when studying entrepreneurial intentions. This research article uses a novel approach, fsQCA, to explore the factors that influence entrepreneurial behaviour. It departs from traditional assumptions that the same causal mechanisms apply to all individuals within a group, and instead examines how shared characteristics may influence entrepreneurial behaviour.
Differently Abled Entrepreneurs: A Systematic Literature Review on Fifty Years of Research—Exploring Thoughts and Debate with Reference to Entrepreneurship
Authors: Sodhi Simran , Dwivedi Amit K
Published: March 2024

Entrepreneurship in disadvantaged sections of society has always been in focus. Researchers have explored the entrepreneurship development phenomenon in various disadvantaged groups, namely tribal, rural, native, women, differently abled and other backward sections of society. There is an emergent stream of literature that explores the phenomenon among people with disabilities. Though in its infancy, this area has enriched entrepreneurship research in the past 50 years. This article aims to map and review the literature on differently abled entrepreneurs, with 130 articles contributing to this field. It highlights details about the top publications, major citations, authors, institutions and countries that have contributed over the years. Beyond a comprehensive bibliometric overview, the article performs a thematic analysis and identifies a spectrum of eight major themes, highlighting a pathway for future studies. Lastly, the implications for enhancing the adoption of entrepreneurship among the differently abled are discussed in brief.
Insights From Commodity Markets Reaction to the Russia–Ukraine War
Authors: Kaur Harpreet , Chaudhary Amita
Published: March 2024

The article aims to investigate how the Russia–Ukraine war has affected India’s base metal, energy, and bullion markets using short-term event technique. The official website of Multi Commodity Exchange of India Limited (MCX) has provided the daily prices for nine specific commodities and selected commodity indexes. The study discovered that the two countries’ conflict had no discernible effect on the Cumulative Abnormal Returns (CARs) of a few selected indices. However, more in-depth examination of specific commodities showed that the CAR of just the base metal index was considerably impacted. The study also observed that the war had an impact on returns of investors who experienced positive returns only in case of nickel whereas negative returns in case of copper, lead and zinc. However, analysis of the cumulative average abnormal return (CAAR) of the selected indices has revealed a consistent and significant negative impact which could be due to other external factors influencing prices of the commodity index and individual indices. The present study is quite useful to investors, traders, analysts, policy makers and risk managers as it assists them to make informed investment decisions, manage risk, identify potential opportunities in the market and develop trading strategies.
An Investigation of Antecedents and Outcomes of Accounting Information Quality: Evidence from SMEs
Authors: Afifa Malik Abu , Nguyen Nha Minh
Published: March 2024

This article aims to scrutinize the antecedents and outcomes of accounting information quality (AIQ) of small- and medium-sized enterprises (SMEs) in the developing country of Vietnam. Based on the perspectives of agency theory and contingency theory, accounting information system quality (AISQ) has been considered as the main antecedent factor, and firm performance (FIP) is examined as the outcome in this article. We additionally examine the mediating role of AISQ in the context of information technology risks (ITR)–AIQ nexus. Furthermore, the moderating role of transformational leadership (TL) was considered in the context of the AISQ–AIQ nexus, and the AIQ–FIP nexus, respectively. Using the quantitative research methods with rigorous testing to determine the most accurate results and ensure reliability, this article concludes that the ITR have an appreciable influence on AISQ, and the AISQ has an appreciable favourable impact on AIQ. Additionally, the AIQ has an appreciable impact on FIP in the context of Vietnamese SMEs, and at the same time, AIQ positively mediates the AISQ–FIP nexus. Lastly, TL positively moderates the AIQ–FIP nexus.
Varanasi—The Making of a Smart Heritage City
Authors: Das Diganta , K. Chowdhary Bikramaditya , Mishra Swasti Vardhan , Aditi Anwesha
Published: March 2024

One of the oldest cities in the world, Varanasi is home to more than one religion. It is an acclaimed centre of spirituality for Hindus and Buddhists while being equally sacred for Jains and Sikhs. The city is located along the river Ganga, which many believe is a celestial incarnation to grant salvation. Varanasi is a million-plus-population city; an increasingly important provincial metropolitan centre enshrined with the values of commerce, religion, ritual and tradition; and caught between the confluence and contestations of traditions and modernity. Since 2016, Varanasi has been incorporated under India’s Smart Cities Mission (SCM) to develop the city into a smart city with a strong focus on heritage and tourism. The present article is an attempt to trace the journey of the city that has developed as a palimpsest over centuries; it deliberates the contemporary urban landscape and maps out recent urban and infrastructure developments of the city and its concerns. This article also argues that Varanasi has focused on locally specific everyday urbanisms with heritage and culture and pivoted to making itself a Smart Heritage City.
Land Price Variability Assessment of a Transport Infrastructure Project in Pabna District, Bangladesh
Authors: Rana Md. Sohel , Rasel Md. , Hasan Md. Hasibul , Tabassum Tasnim
Published: March 2024

Transport infrastructure development projects play a vital role in improving accessibility, which has dynamic effects on the regional economy as well as increases property values. This research aims to assess the land price variability in control and catchment areas before and after the Dasuria-to-Dhalarchar railway development project in Pabna district, Bangladesh. A set of independent variables was considered to measure the correlation between development project and land price through a questionnaire survey and focus group discussion. The impact of a railway project on land price was determined by the ordinary least squares method, after which the method was justified through Cook’s distance, P-P plot and scatter plot. The data analysis reveals a strong association between land price and distance to a central business district. The data shows that the land price increased significantly after implementation of the railway project with different intensities, as functional characteristics vary among selected stations.
Self-management Support for Patients with Atopic Dermatitis: A Qualitative Interview Study
Authors: Krontoft Anna Sophie Belling , Skov Lone , Ammitzboell Elisabeth , Lomborg Kirsten
Published: March 2024

Patients with a chronic skin disease, eg, atopic dermatitis, need self-management skills to increase their quality of life. We explored patients’ needs for self-management support from healthcare professionals and how these needs can be met in a dermatology setting. Interpretive description methodology was chosen for iterative data collection and analysis of qualitative interviews with patients with atopic dermatitis. Two mutually dependent themes were found to be supportive of patients’ self-management. Personal and disease-related recognition was fundamental to successful support. However, guidance for agenda-setting from healthcare professionals was also needed on the wide range of topics that could be covered in the consultation based on individual needs. Patients need self-management support in addition to what can be found with family, friends, or peers. It is crucial that the support is delivered with an appreciative approach by healthcare professionals with profound knowledge of atopic dermatitis. Equally important is guidance towards agenda-setting, a way to co-construct the consultation with a clear focus on the specific patient's needs.
Effect-based monitoring of chemical hazards in drinking water from source to tap: seasonal trends over 2 years of sampling
Authors: Lundqvist Johan , Lavonen Elin , Mandava Geeta , Selin Erica , Ejhed Helene , Oskarsson Agneta
Published: March 2024

Drinking water producers have a far-reaching responsibility to provide safe, clean and wholesome drinking water, using water resources possibly effected by the thousands of chemicals used in societies’ daily life. This study has monitored chemical hazards in drinking water from source to tap, using effect-based methods. The study was conducted at a Swedish drinking water treatment plant sourcing lake water and aimed to investigate potential seasonal variations in both the raw water and water after different treatment steps. Furthermore, a granular activated carbon (GAC) pilot facility was evaluated. Samples were analyzed for estrogenicity, androgenicity, aryl hydrocarbon receptor (AhR) activity, oxidative stress (Nrf2) response and genotoxicity (micronucleus formation). We observed seasonal differences in oxidative stress and genotoxic effects in both raw and drinking water with higher activities during the late fall of each year. The removal efficiency for both oxidative stress and genotoxicity was limited in the full-scale treatment process and occasionally the genotoxicity was also detected in outgoing drinking water from the treatment plant and in samples collected at consumers tap on the distribution network. AhR activity was present in all raw water samples and the removal was limited. Estrogenic activities were observed in most of the raw water samples but in contrast to the other parameters estrogenicity was effectively reduced by the full-scale conventional treatment. The GAC pilot treatment was generally more efficient than the full-scale conventional treatment in removing all observed bioactivities and could be a viable complement to the current treatment to assure drinking water free from genotoxic compounds. Genotoxic activities in drinking water was observed while all currently regulated chemical parameters were fulfilled. This highlights the need for effect-based monitoring in efforts to ensure the chemical safety of drinking water, as target chemical analysis of single compounds will overlook both unknown hazardous compounds as well as potential mixture effects.

Graphical Abstract
Efficacy of acupuncture as an adjunctive treatment to patients with stable COPD: a multicenter, randomized, sham-controlled trial protocol
Authors: Huang Liuyang , Yang Sha , Xu Guixing , Luo Qin , Yang Chunyan , Tian Hao , Liu Yilin , Zhou Zhuo , Huang Fengyuan , Gong Siyao , Li Qian , Yu Xin , Chen Ming , Huang Dan , Liu Yunyu , Tang Juan , Zhang Ruixin , Sun Xin , Lu Guangbing , Zeng Chunfang , Ai Shuangchun , Li Bin , Chen Jian qin , Luo Quan , Xiong Chan , Zou Zhi , Hu Qiang , Luo Xiaochao , Li Ling , Sun Mingsheng , Zeng Fang , Liang Fanrong
Published: March 2024

Background

Chronic obstructive pulmonary disease (COPD) is a common respiratory disease and the third leading cause of death worldwide. Previous evidence has shown that acupuncture may be an effective complementary alternative therapy for stable COPD. However, large-sample, rigorously designed long-term follow-up studies still need to be completed. Notably, the relationship between the frequency of acupuncture and clinical efficacy in studies on acupuncture for stable COPD still needs further validation. This study aims to evaluate the efficacy and safety of acupuncture for stable COPD and further investigate the dose–effect relationship of acupuncture.

Methods/design

This is a multicenter, randomized, controlled trial that uses central randomization to randomly allocate 550 participants in a 1:1:1:1:1 ratio to once a week acupuncture group, twice a week acupuncture group, three times a week acupuncture group, sham acupuncture group and waiting-list control group. The sham acupuncture group will receive placebo acupuncture treatments three times per week, and the waiting-list control group will not receive any form of acupuncture intervention. The study consists of a 2-week baseline, 12-week of treatment, and 52-week of follow-up. Patients with COPD between 40 to 80 years old who have received stable Western medication within the previous 3 months and have had at least 1 moderate or severe acute exacerbation within the past 1 year will be included in the study. Basic treatment will remain the same for all participants. The primary outcome is the proportion of responders at week 12. Secondary outcomes include the proportion of responders at week 64, change in the St. George's Respiratory Questionnaire (SGRQ) Scale, change in the Modified-Medical Research Council (mMRC) Scale, change in the COPD Assessment Test (CAT) Scale, change in the Lung Function Screening Indicators (LFSI), change in the 6-min walk distance (6-MWD), change in Short-Form 36 Health Survey (SF-36) Scale, the number of moderate and severe acute exacerbations and adverse event rate during the follow-up period.

Discussion

This study will provide robust evidence on whether acupuncture is safe and effective for treating stable COPD. Meanwhile, comparing the differences in efficacy between different acupuncture frequencies will further promote the optimization of acupuncture for stable COPD.

Trial registration

This study was registered in the Chinese Clinical Trial Registry (ChiCTR2200058757), on April 16, 2022.
Learning to teach with patients and caregivers: a focused ethnography
Authors: Alberti Sara , Vannini Valeria , Ghirotto Luca , Bonetti Loris , Rovesti Sergio , Ferri Paola
Published: March 2024

Background

Little is known about what happens when patients and caregivers are involved in an academic setting as co-teachers and how healthcare professionals approach a new model of partnership-based teaching. This study aimed to explore the learning and behavioural patterns of a group of healthcare professionals who were learning to teach with patients and caregivers as co-teachers in a post-graduate course.

Methods

A focused ethnographic study involving 11 health professionals was conducted. Data were collected through participatory observation during the course, individual semi-structured interviews, and a follow-up focus group. Taxonomic analysis was performed.

Results

Three categories were identified: ‘group’, ‘role of narration’ and ‘applying co-teaching with patients and caregivers ’. Specifically, heterogeneity, absence of hierarchies, and balanced relationships characterised the group dynamic and promoted partnership. Narration played a key role both in learning and in healthcare professionals’ relationship with patients and caregivers and promoted emotional skills and self-awareness. Project planning and lessons simulations were essential aspects of the implementation process.

Conclusions

This focused ethnography helped further understanding of the context of a specific project involving patients and caregivers as co-teachers in healthcare professional education. The development of emotional skills and self-awareness are the main learning patterns of co-teaching, and interprofessionalism and balanced relationships are the basis of the behavioural patterns. These patterns facilitated the involvement of patients and caregivers in health education.
Natural language processing to identify lupus nephritis phenotype in electronic health records
Authors: Deng Yu , Pacheco Jennifer A. , Ghosh Anika , Chung Anh , Mao Chengsheng , Smith Joshua C. , Zhao Juan , Wei Wei-Qi , Barnado April , Dorn Chad , Weng Chunhua , Liu Cong , Cordon Adam , Yu Jingzhi , Tedla Yacob , Kho Abel , Ramsey-Goldman Rosalind , Walunas Theresa , Luo Yuan
Published: March 2024

Background

Systemic lupus erythematosus (SLE) is a rare autoimmune disorder characterized by an unpredictable course of flares and remission with diverse manifestations. Lupus nephritis, one of the major disease manifestations of SLE for organ damage and mortality, is a key component of lupus classification criteria. Accurately identifying lupus nephritis in electronic health records (EHRs) would therefore benefit large cohort observational studies and clinical trials where characterization of the patient population is critical for recruitment, study design, and analysis. Lupus nephritis can be recognized through procedure codes and structured data, such as laboratory tests. However, other critical information documenting lupus nephritis, such as histologic reports from kidney biopsies and prior medical history narratives, require sophisticated text processing to mine information from pathology reports and clinical notes. In this study, we developed algorithms to identify lupus nephritis with and without natural language processing (NLP) using EHR data from the Northwestern Medicine Enterprise Data Warehouse (NMEDW).

Methods

We developed five algorithms: a rule-based algorithm using only structured data (baseline algorithm) and four algorithms using different NLP models. The first NLP model applied simple regular expression for keywords search combined with structured data. The other three NLP models were based on regularized logistic regression and used different sets of features including positive mention of concept unique identifiers (CUIs), number of appearances of CUIs, and a mixture of three components (i.e. a curated list of CUIs, regular expression concepts, structured data) respectively. The baseline algorithm and the best performing NLP algorithm were externally validated on a dataset from Vanderbilt University Medical Center (VUMC).

Results

Our best performing NLP model incorporated features from both structured data, regular expression concepts, and mapped concept unique identifiers (CUIs) and showed improved F measure in both the NMEDW (0.41 vs 0.79) and VUMC (0.52 vs 0.93) datasets compared to the baseline lupus nephritis algorithm.

Conclusion

Our NLP MetaMap mixed model improved the F-measure greatly compared to the structured data only algorithm in both internal and external validation datasets. The NLP algorithms can serve as powerful tools to accurately identify lupus nephritis phenotype in EHR for clinical research and better targeted therapies.
Developing and evaluating an evidence-based practice research competency enhancement program for clinical nurses in Korea: a pilot study
Authors: Kim Suhyun , Jeong Hye Won
Published: March 2024

Background

Evidence-based practice (EBP) is crucial for delivering high-quality healthcare and effective self-care. Enhancing clinical nurses’ research competencies through structured mentorship is key to the widespread application of EBP. This study evaluated a newly developed Research Competency Enhancement Program (RCEP), aimed at bolstering EBP among experienced nurses.

Methods

Conducted in a tertiary university hospital in Korea, this single-group study employed a pretest-post-test design and a mixed-methods approach. The RCEP involved 11 experienced clinical nurses in an 8-week intervention, featuring mentor-led workshops, interactive sessions, and resource-driven activities. Data were collected using the Evidence-Based Practice Beliefs Scale (EBPB), the Evidence-Based Practice Attitude Scale (EBPA), and the Research Practice Ability (RPA) tool, alongside qualitative feedback. These measures assessed the program’s feasibility, acceptability, and preliminary effectiveness.

Results

The quantitative analysis indicated significant improvements in research competency post-intervention. Mean scores on the EBPB and RPA scales increased (Z = -2.53, p = .011; Z = -2.66, p = .008). Participants described the RCEP as inspirational and challenging, creating an environment conducive to research. Facilitators included mentor support and innovative learning tools, while barriers were internet connectivity and scheduling conflicts. Suggestions for improvement included more hands-on sessions, small team collaborations, and integration with academic institutions.

Conclusion

The RCEP, facilitated by EBP mentors, significantly improved the research competencies and attitudes of clinical nurses towards EBP. The study underscores the importance of continual RCEP refinement, integrating structured, interactive, and collaborative elements to further empower nurses in evidence-based practice. The program shows promise in enhancing research competencies and fostering a commitment to EBP in clinical settings.
Critical care nurses’ experiences of caring challenges during post-resuscitation period: a qualitative content analysis
Authors: Zali Mahnaz , Rahmani Azad , Hassankhani Hadi , Namdar-Areshtanab Hossein , Gilani Neda , Azadi Arman , Ghafourifard Mansour
Published: March 2024

Background

Patients in the post-resuscitation period experience critical conditions and require high-quality care. Identifying the challenges that critical care nurses encounter when caring for resuscitated patients is essential for improving the quality of their care.

Aim

This study aimed to identify the challenges encountered by critical care nurses in providing care during the post-resuscitation period.

Methods

A qualitative study was conducted using semi-structured interviews. Sixteen nurses working in the intensive care units of three teaching hospitals were selected through purposive sampling. The Data collected were analyzed using qualitative content analysis.

Results

Participants experienced individual, interpersonal, and organizational challenges when providing post-resuscitation care. The most significant challenges include inadequate clinical knowledge and experience, poor management and communication skills, lack of support from nurse managers, role ambiguity, risk of violence, and inappropriate attitudes of physicians towards nurses’ roles. Additionally, nurses expressed a negative attitude towards resuscitated patients.

Conclusion

Critical care nurses face several challenges in providing care for resuscitated patients. To enhance the quality of post-resuscitation care, address the challenges effectively and improve long-time survival it is crucial to implement interventions such as In-service education, post-resuscitation briefing, promotion of interprofessional collaboration among healthcare teams, providing sufficient human resources, clarifying nurses’ roles in the post-resuscitation period and increasing support from nursing managers.
Automated workflow for characterization of bacteriocin production in natural producers Lactococcus lactis and Latilactobacillus sakei
Authors: Steier Valentin , Prigolovkin Lisa , Reiter Alexander , Neddermann Tobias , Wiechert Wolfgang , Reich Sebastian J. , Riedel Christian U. , Oldiges Marco
Published: March 2024

Background

Lactic acid bacteria are commonly used as protective starter cultures in food products. Among their beneficial effects is the production of ribosomally synthesized peptides termed bacteriocins that kill or inhibit food-spoiling bacteria and pathogens, e.g., members of the Listeria species. As new bacteriocins and producer strains are being discovered rapidly, modern automated methods for strain evaluation and bioprocess development are required to accelerate screening and development processes.

Results

In this study, we developed an automated workflow for screening and bioprocess optimization for bacteriocin producing lactic acid bacteria, consisting of microcultivation, sample processing and automated antimicrobial activity assay. We implemented sample processing workflows to minimize bacteriocin adsorption to producer cells via addition of Tween 80 and divalent cations to the cultivation media as well as acidification of culture broth prior to cell separation. Moreover, we demonstrated the applicability of the automated workflow to analyze influence of media components such as MES buffer or yeast extract for bacteriocin producers Lactococcus lactis B1629 and Latilactobacillus sakei A1608.

Conclusions

Our automated workflow provides advanced possibilities to accelerate screening and bioprocess optimization for natural bacteriocin producers. Based on its modular concept, adaptations for other strains, bacteriocin products and applications are easily carried out and a unique tool to support bacteriocin research and bioprocess development is provided.
EphA2-specific microvesicles derived from tumor cells facilitate the targeted delivery of chemotherapeutic drugs for osteosarcoma therapy
Authors: Wang Zhenggang , He Zhiyi , Wan Junlai , Chen Anmin , Cheng Peng , Zhu Wentao
Published: March 2024

Despite advances in surgery and chemotherapy, the survival of patients with osteosarcoma (OS) has not been fundamentally improved over the last two decades. Microvesicles (MVs) have a high cargo-loading capacity and are emerging as a promising drug delivery nanoplatform. The aim of this study was to develop MVs as specifically designed vehicles to enable OS-specific targeting and efficient treatment of OS. Herein, we designed and constructed a nanoplatform (YSA-SPION-MV/MTX) consisting of methotrexate (MTX)-loaded MVs coated with surface-carboxyl Fe3O4 superparamagnetic nanoparticles (SPIONs) conjugated with ephrin alpha 2 (EphA2)-targeted peptides (YSAYPDSVPMMS, YSA). YSA-SPION-MV/MTX showed an effective targeting effect on OS cells, which was depended on the binding of the YSA peptide to EphA2. In the orthotopic OS mouse model, YSA-SPION-MV/MTX effectively delivered drugs to tumor sites with specific targeting, resulting in superior anti-tumor activity compared to MTX or MV/MTX. And YSA-SPION-MV/MTX also reduced the side effects of high-dose MTX. Taken together, this strategy opens up a new avenue for OS therapy. And we expect this MV-based therapy to serve as a promising platform for the next generation of precision cancer nanomedicines.

Graphical Abstract
CDSKNNXMBD: a novel clustering framework for large-scale single-cell data based on a stable graph structure
Authors: Ren Jun , Lyu Xuejing , Guo Jintao , Shi Xiaodong , Zhou Ying , Li Qiyuan
Published: March 2024

Background

Accurate and efficient cell grouping is essential for analyzing single-cell transcriptome sequencing (scRNA-seq) data. However, the existing clustering techniques often struggle to provide timely and accurate cell type groupings when dealing with datasets with large-scale or imbalanced cell types. Therefore, there is a need for improved methods that can handle the increasing size of scRNA-seq datasets while maintaining high accuracy and efficiency.

Methods

We propose CDSKNNXMBD (Community Detection based on a Stable K-Nearest Neighbor Graph Structure), a novel single-cell clustering framework integrating partition clustering algorithm and community detection algorithm, which achieves accurate and fast cell type grouping by finding a stable graph structure.

Results

We evaluated the effectiveness of our approach by analyzing 15 tissues from the human fetal atlas. Compared to existing methods, CDSKNN effectively counteracts the high imbalance in single-cell data, enabling effective clustering. Furthermore, we conducted comparisons across multiple single-cell datasets from different studies and sequencing techniques. CDSKNN is of high applicability and robustness, and capable of balancing the complexities of across diverse types of data. Most importantly, CDSKNN exhibits higher operational efficiency on datasets at the million-cell scale, requiring an average of only 6.33 min for clustering 1.46 million single cells, saving 33.3% to 99% of running time compared to those of existing methods.

Conclusions

The CDSKNN is a flexible, resilient, and promising clustering tool that is particularly suitable for clustering imbalanced data and demonstrates high efficiency on large-scale scRNA-seq datasets.
Imported allergens in Italy: an emerging issue
Authors: Pecoraro Luca , Giovannini Mattia , Mori Francesca , Barni Simona , Castagnoli Riccardo , Arasi Stefania , Mastrorilli Carla , Saretta Francesca , Liotti Lucia , Caminiti Lucia , Klain Angela , Gelsomino Mariannita , Del Giudice Michele Miraglia , Novembre Elio
Published: March 2024

Imported allergens are involved in many allergic reactions, with unexpected and unusual implications. They can be involved in developing asthma, allergic rhinoconjunctivitis, Hymenoptera venom allergies and food allergies. Imported allergens can be implied in respiratory allergies attributable to commercial practices and accidental diffusion through air currents that have introduced non-native species in new geographical contexts. Ambrosia artemisiifolia L., a plant native to North America and currently in the western part of Lombardy, represents an example. Moreover, a variation in the pollen concentration in the Northwest Tuscany area and Trentino Alto-Adige was observed. Cannabis sativa is another imported allergen used frequently by adolescents. Regarding potential imported food allergens, there is no validated list. Imported food allergens derive from ethnic foods, referring to Mexican/Latin American, Chinese/Japanese, Southeast Asian, Arab/Middle Eastern and African cuisine. Four insect flours were recently introduced to the European and Italian markets (Acheta domesticus, Alphitobius diaperinus, Tenebrio molitor and Locusta migratoria). The association between the accidental introduction through commercial traffic, climate change, and the absence of natural enemies in the destination ecosystem is related to the introduction of a specific Hymenoptera, Vespa velutina, in Italy and Europe. External events attributable to human activities, such as climate change and the introduction of non-native plants, foods and Hymenoptera through trade, have contributed to the issue of imported allergens. Making the correct diagnosis and guiding the diagnostic and therapeutic path in this particular context represent the concerns of the pediatric allergist.
Rock music improvisation shows increased activity in Broca’s area and its right hemisphere homologue related to spontaneous creativity
Authors: Tachibana Atsumichi , Noah J. Adam , Ono Yumie , Irie Shun , Tatsumoto Muneto , Taguchi Daisuke , Tokuda Nobuko , Ueda Shuichi
Published: March 2024

Objective

The neural correlates of creativity are not well understood. Using an improvised guitar task, we investigated the role of Broca's area during spontaneous creativity, regardless of individual skills, experience, or subjective feelings.

Results

Twenty guitarists performed improvised and formulaic blues rock sequences while hemodynamic responses were recorded using functional near-infrared spectroscopy. We identified a new significant response in Broca’s area (Brodmann area [BA] 45L) and its right hemisphere homologue during improvised playing but not during formulaic playing. Our results indicate that bilateral BA45 activity is common during creative processes that involve improvisation across all participants, regardless of subjective feelings, skill, age, difficulty, history, or amount of practice. While our previous results demonstrated that the modulation of the neural network according to the subjectively experienced level of creativity relied on the degree of deactivation in BA46L, our current results independently show a common concurrent activity in BA45 in all participants. We suggest that this is related to the sustained execution of improvisation in “motor control,” analogous to motor planning in speech control.
Features of biliary tract diseases in ketamine abusers: a systematic review of case reports
Authors: Teymouri Alireza , Nasoori Hadis , Fakheri Maryamsadat , Nasiri Aref
Published: March 2024

Background and aims

Anesthesiologists prefer ketamine for certain surgeries due to its effectiveness as a non-competitive inhibitor of the N-methyl-D-aspartate receptor in the brain. Recently, this agent has also shown promise as an antidepressant. However, ketamine can cause hallucinogenic effects and is sometimes abused as an illicit drug. Ketamine abuse has been associated with liver and bile duct complications. This systematic study aims to better understand cholangiopathy in ketamine abusers by reviewing case reports.

Methods and material

In this systematic review, a comprehensive literature search was conducted with the terms “biliary tract diseases” and “ketamine”. Case reports and case series of adult patients with documented ketamine abuse and reported cholangiopathy or biliary tract disease were included. We extracted the data of relevant information and the results were reported through narrative synthesis and descriptive statistics.

Results

A total of 48 studies were initially identified, and 11 studies were finally included in the review. The mean age of the patients was 25.88 years. Of the 17 patients, 64.7% were men. Symptoms often included abdominal pain, nausea, and vomiting. Most patients were discharged with improved symptoms and liver function. Common bile duct dilation and other findings were observed in imaging results and other diagnostic studies.

Conclusion

This review highlights the diverse presentations and diagnostic modalities used in ketamine-induced cholangiography. These patients tend to be young men with deranged liver function tests and abdominal pain, which should be taken into consideration. These patients often require a multidisciplinary approach in their management.
Improving hematopoietic differentiation from human induced pluripotent stem cells by the modulation of Hippo signaling with a diarylheptanoid derivative
Authors: Thongsa-ad Umnuaychoke , Wongpan Anongnat , Wongkummool Wasinee , Chaiwijit Phaewa , Uppakara Kwanchanok , Chaiyakitpattana Gorawin , Singpant Passanan , Tong-ngam Pirut , Chukhan Amnat , Pabuprappap Wachirachai , Wongniam Sirapope , Suksamrarn Apichart , Hongeng Suradej , Anurathapan Usanarat , Kulkeaw Kasem , Tubsuwan Alisa , Bhukhai Kanit
Published: March 2024

Background

The diarylheptanoid ASPP 049 has improved the quality of adult hematopoietic stem cell (HSC) expansion ex vivo through long-term reconstitution in animal models. However, its effect on hematopoietic regeneration from human induced pluripotent stem cells (hiPSCs) is unknown.

Method

We utilized a defined cocktail of cytokines without serum or feeder followed by the supplementation of ASPP 049 to produce hematopoietic stem/progenitor cells (HSPCs). Flow cytometry and trypan blue exclusion analysis were used to identify nonadherent and adherent cells. Nonadherent cells were harvested to investigate the effect of ASPP 049 on multipotency using LTC-IC and CFU assays. Subsequently, the mechanism of action was explored through transcriptomic profiles, which were validated by qRT-PCR, immunoblotting, and immunofluorescence analysis.

Result

The supplementation of ASPP 049 increased the number of phenotypically defined primitive HSPCs (CD34+CD45+CD90+) two-fold relative to seeded hiPSC colonies, indicating enhanced HSC derivation from hiPSCs. Under ASPP 049-supplemented conditions, we observed elevated HSPC niches, including CD144+CD73− hemogenic- and CD144+CD73+ vascular-endothelial progenitors, during HSC differentiation. Moreover, harvested ASPP 049-treated cells exhibited improved self-renewal and a significantly larger proportion of different blood cell colonies with unbiased lineages, indicating enhanced HSC stemness properties. Transcriptomics and KEGG analysis of sorted CD34+CD45+ cells-related mRNA profiles revealed that the Hippo signaling pathway is the most significant in responding to WWTR1/TAZ, which correlates with the validation of the protein expression. Interestingly, ASPP 049-supplemented HSPCs upregulated 11 genes similarly to umbilical cord blood-derived HSPCs.

Conclusion

These findings suggest that ASPP 049 can improve HSC-generating protocols with proliferative potentials, self-renewal ability, unbiased differentiation, and a definable mechanism of action for the clinical perspective of hematopoietic regenerative medicine.

Graphical abstract