bluuebunny/crossref_metadata_2025 huggingface dataset:
Gut Health and Its Association with Wellbeing and Nutrient Intake in Community-Dwelling Older Adults
Authors: Fart Frida , Tingö Lina , Engelheart Stina , Lindqvist Carl Mårten , Brummer Robert J. , Kihlgren Annica , Schoultz Ida
Published: October 2022

Many of the increasing number of community-dwelling older adults will need increased healthcare in the future. By characterising gut health and its association with wellbeing and nutrient intake in this population, we aim to recognise areas along the gut–brain axis through which the health of community-dwelling older adults might be promoted. In this cross-sectional observational study, validated questionnaires were used to assess gut health, nutrient intake, and wellbeing in 241 community-dwelling older adults (≥65 years old). In total, 65% of the participants experienced at least one gastrointestinal symptom, of which females had more abdominal pain and constipation, while the oldest old (i.e., ≥80 years old) had more diarrhoea. Increased gastrointestinal symptoms correlated with more stress, anxiety, depression, and a decreased quality of life, in addition to dyspepsia which correlated with a lower E% of protein. Most of the participants did not reach the recommended intake for protein, fibre and polyunsaturated fats. Males had a lower intake of protein (E%) and fibre (g/MJ) than females, and the oldest old had a lower E% of protein than younger older adults. In conclusion, our results demonstrate that gastrointestinal symptoms are common, and most of the study participants had an imbalanced macronutrient intake, which could be a target for future possible dietary interventions to improve overall health.
Representations of Light in Western Culture
Authors: Nir Bina
Published: October 2022

In physical terms, light is a wave radiating from an energy source, yet different cultures in different periods have also attributed metaphysical properties to light that are outside of nature. Even in today’s secular discourse, we often resort to using imagery of light to symbolise a variety of virtues, whereas ‘New Age’ discourse raises light to a renewed metaphysical status. In this article, we will use the genealogical method to examine the origins of the popular Western conception of light as representative of knowledge, goodness, wisdom and sanctity by looking at the great myths and the foundational texts of Western culture. This understanding of light is a deep structure, originating in religion, that persists in secular culture: from ancient Near Eastern mythologies, to Plato’s parable of the cave, to the Judeo-Christian narrative and the Enlightenment and culminating in the role of light in New Age culture.
Screening for Monkeypox Infection in Asymptomatic High-Risk-Behaviour Men Having Sex with Men (MSM)
Authors: Pestel Julia , Matthews Hanna , Schmiedel Stefan , Hüfner Anja , Jordan Sabine , Scheiter Robin Luca , Lütgehetmann Marc , Nörz Dominik , Degen Olaf
Published: October 2022

Background: Since the outbreak of monkeypox in formerly non-endemic countries, we have included a screening for monkeypox in our sexually transmitted diseases (STD) routine in patients with high-risk behavior, as it is mainly transmitted through close skin to mucous membrane contact with infected individuals. Methods: Between 16 June 2022 and 14 July 2022, we screened 53 MSM with high-risk behavior for monkeypox acquisition in an observational prospective cohort trial. We complemented the throat and anal swabs for chlamydia and gonococci with monkeypox using polymerase chain reaction (PCR). In addition, all patients participated in a questionnaire survey about their risk behavior and previous STD in their medical history. Results: None of the 53 participants had tested positive for the monkeypox virus. One patient was diagnosed with syphilis and one with an oral and anorectal chlamydia infection. Conclusions: STD screening in high-risk populations is a valuable tool to detect asymptomatic patients for chlamydia, gonococci, HIV, hepatitis B and C and syphilis. Based on our small cohort, monkeypox screening in asymptomatic MSM patients in areas of low prevalence does not seem to be an appropriate approach to deal with the ongoing outbreak. Therefore, we recommend to focus more on vaccinations, targeted nonstigmatizing information and behavior recommendation for risk populations, and to engage further investigations.
Predicting Physical Activity in Chinese Pregnant Women Using Multi-Theory Model: A Cross-Sectional Study
Authors: Zhang Wei , Jin Ying , Liu Ningning , Xiang Zhenzhen , Wang Xiaojuan , Xu Ping , Guo Pingping , Mao Minna , Feng Suwen
Published: October 2022

Background: Physical activity (PA) brings many benefits to pregnant women and fetuses; however, the majority of pregnant women do not participate actively in PA during pregnancy. Objectives: This study aimed to: (1) assess the utility of Multi-Theory Model (MTM) to explain the intentions of PA behavior in Chinese pregnant women; (2) analyze the predictors in initiating and maintaining PA behavior based on MTM. Methods: A cross-sectional study including pregnant women was conducted from March to June 2022 at a university hospital in Hangzhou, Zhejiang Province, China. Participants completed measures that included a self-developed demographic questionnaire and a 29-item MTM questionnaire. Descriptive statistics and stepwise multiple regression were used to analyze the data. The reliability was assessed by Cronbach’s alpha and test-retest stability. The construct validity was evaluated by using exploratory factor (EFA) analysis and confirmatory factor analysis (CFA). Results: A total of 450 pregnant women participated in this study. The score of the magnitude of intention to initiate and maintain PA behavior during pregnancy was 2.30 (1.08) and 2.24 (1.09). The overall Cronbach’s alpha value was 0.857. A four-factor structure for initiation model and a three-factor structure for maintenance model were determined. Results of the CFA confirmed construct validity of subscales (initiation model: χ2 = 206.123, df = 140, p < 0.001, χ2/df = 1.472, RMSEA = 0.046, SRMR = 0.0432, GFI = 0.913, CFI = 0.982; maintenance model: χ2 = 49.742, df = 29, p < 0.001, χ2/df = 1.715, RMSEA = 0.057, SRMR = 0.0432, GFI = 0.958, CFI 0.985). The result of regression indicated that participatory dialogue (β = 0.030; p = 0.002), behavioral confidence (β = 0.128; p < 0.001), changes in physical environment (β = 0.041; p = 0.005), trimester (β = −0.192; p = 0.001), and Gestational Diabetes Mellitus (GDM) (β = 0.408; p < 0.001) explained 52.1% variance in initiating PA behavior. Emotional transformation (β = 0.197; p < 0.001), practice for change (β = 0.083; p = 0.001), changes in social environment (β = 0.063; p < 0.001), pre-pregnancy exercise habit (β = −0.251; p = 0.001), and GDM (β = 0.298; p = 0.003) were significantly associated with pregnant women’s intentions to maintain PA behavior and explained 49.1% variance. Conclusions: The constructs of MTM were effective in explaining the intention to initiate and maintain PA behavior among Chinese pregnant women.
The Direct and Structure Effect of Income on Nutrition Demand of Chinese Rural Residents
Authors: Zeng Qiyan , He Zhipeng , Wang Yuting
Published: October 2022

Although a significant body of literature has analyzed the effect of income-mediated policies on nutrition, research on how income affect nutrition consumption is scant. This paper contributes to the literature by decomposing the overall income effect on rural residents’ calorie intake into the direct income effect and the structure effect by building a simple theoretical model and conducting related empirical research with an instrumental variable (IV) approach. Using nationally representative data from China, we find that the structure effect of income, represented by fat share growth induced by income, occupies a considerable proportion (38.03%) of overall income effect. Additionally, we provide evidence of an asymmetric distributional effect of income on calorie intake. In particular, the structure effect of income substantially accounts for a larger proportion in the higher quantiles of the calorie intake distribution. Our findings help better evaluate the effectiveness of the income-mediated policies from quantity and structure perspectives in a comprehensive framework.
Effects of Sodium Bicarbonate Ingestion on Recovery in High-Level Judokas
Authors: Danković Goran , Stanković Nemanja , Milošević Nikola , Živković Vladimir , Russo Luca , Migliaccio Gian Mario , Larion Alin , Trajković Nebojša , Padulo Johnny
Published: October 2022

Background: The aim of this study was to examine the sodium bicarbonate (NaHCO3) effect on recovery in high-level judokas. Methods: The sample of participants consisted of 10 male judokas (Age = 20 ± 2.1 years) who are judo masters (black belt holders) with a minimum of 10 years of training and competition experience. The study was designed as a double-blinded crossover design with the order of treatments being randomly assigned. The washout period was 72 h. All subjects received a dose of sodium bicarbonate (0.3 g/kg body weight) or a placebo 120 min before the fatigue caused by the special judo fitness test (SJFT). Lactate concentration (LC), countermovement jump (CMJ), hand grip strength and degree of perceived fatigue on Borg’s scale (RPE) were tested two times before SJFT and four times after SJFT. Results: There was no interaction between groups and type of recovery at any time during the two types of recovery for RPE, grip strength, VJ and lactate concentration (p > 0.05). However, there was a main effect of time for dominant grip strength (F(1,8)= 3.3; p = 0.01; η2 = 0.25, (small)), non-dominant grip strength (F(1,8) = 3.2; p = 0.01; η2 = 0.24, (small)), CMJ (F(1,8) = 8.8; p = 0.01; η2 = 0.47, (small)), and LC (F(1,8) = 124.2; p = 0.001; η2 = 0.92, (moderate)). Conclusions: The results of the present study show no significant difference between the NaHCO3 and placebo groups in RPE, handgrip strength, CMJ, and lactate concentration.
Classroom Digital Teaching and College Students’ Academic Burnout in the Post COVID-19 Era: A Cross-Sectional Study
Authors: Song Wenlong , Wang Zihan , Zhang Ruiqing
Published: October 2022

The continued development of digital technology and its overuse in teaching and learning in the post-epidemic era have brought about digital health risks, which are associated with academic burnout among college students. This study focused on the relationship between classroom digital teaching and students’ academic burnout and designed the Classroom Burnout Inventory (CBI) and the Classroom Burnout Causes Inventory (CBCI) to conduct a cross-sectional survey of 206 Chinese university students. Correlations and regression analyses were conducted between key factors and burnout values through a path model of “Digital teaching-Teaching & learning process-Causes subjects-Burnout”. The results of the study show that an inappropriate and excessive use of unintegrated digital teaching and learning technologies in the classroom was positively correlated with academic burnout among college students. Burnout levels and the three manifestations were not correlated with students’ gender, grade, and major. In terms of causes, the academic burnout of college students was more correlated with their own personal reasons than with external factors such as teachers, universities, and environments. Integrating digital technology platforms, enhancing teacher leadership in the digital classroom, and strengthening peer support and students’ psychological resilience are all meaningful explorations of academic burnout prevention strategies.
The Associations between Meeting 24-Hour Movement Guidelines (24-HMG) and Self-Rated Physical and Mental Health in Older Adults—Cross Sectional Evidence from China
Authors: Luo Lin , Cao Yunxia , Hu Yulong , Wen Shaojing , Tang Kaiqi , Ding Lina , Song Naiqing
Published: October 2022

Background: This study determined the prevalence of older adults (aged 60–80 years) meeting the Canadian 24-HMG alone and in combination, and their association with the self-rated physical health and mental health of older adults. Methods: Participants were drawn from 4134 older adults aged 60–80 years (age, 67.37 ± 0.08 years; 46.87% male) from the China Health and Nutrition Survey (CHNS) 2015 database. Mental health and physical health scores were obtained based on self-rated mental health questionnaires and physical health of older adults. The criteria for meeting the 24-HMG were: physical activity time ≥ 150 min/week, sedentary time ≤ 480 min/day, screen time ≤ 180 min/day, sleep time 7–9 h (60–64 years) and sleep time 7–8 h (65 years and above). Logistic regression models were used to examine the association between meeting the 24-HMG guidelines number and category and older adults’ self-rated mental and physical health. Results: The proportion of older people meeting three of the 24-HMG guidelines was 1.16%, the proportion meeting two guidelines was 38.19% and the proportion meeting one guideline was 49.14%. The number of people meeting 24-HMG was closely related to the self-rated physical health and mental health of older people. The category meeting 24-HMG was closely related to the self-rated physical health and mental health of older people. Conclusions: Meeting more guidelines in the 24-HMG was strongly associated with older people’s self-rated mental health and physical health. However, only a small proportion of older people met all the recommendations, highlighting the need to promote and support adherence to these movement behaviours.
Mediating Effects of Discipline Approaches on the Relationship between Parental Mental Health and Adolescent Antisocial Behaviours: Retrospective Study of a Multisystemic Therapy Intervention
Authors: Nuntavisit Leartluk , Porter Mark
Published: October 2022

Poor parental mental health is one of the risk factors for child emotional and behavioural problems because it reduces caregiver’s ability to provide appropriate care for their child. This study aimed to measure changes in parenting factors and adolescent behaviours after Multisystemic Therapy (MST), and to explore the mediating role of discipline approaches on the relationship between parental mental health and adolescent behavioural problems. This retrospective study extracted data collected from 193 families engaged with the MST research program during 2014–2019. Data was collected at different time points (pre-treatment, post-treatment, 6- and 12-months follow-up). Statistically significant changes were found in adolescent behaviours and parenting factors following the MST intervention and these positive changes were maintained over the following 12 months. Results of the parallel multiple mediator model analysis confirmed mediating effects of discipline approaches on the relationship between parental mental health and adolescent’s behavioural problems. The findings suggested that parental mental well-being significantly contributes to effectiveness of parenting, which resulted in positive changes in adolescent’s behavioural problems. It is recommended caregiver’s parental skills and any mental health issues are addressed during the intervention to enhance positive outcomes in adolescent behaviour.
Do Loneliness and Per Capita Income Combine to Increase the Pace of Biological Aging for Black Adults across Late Middle Age?
Authors: Beach Steven R. H. , Klopack Eric T. , Carter Sierra E. , Philibert Robert A. , Simons Ronald L. , Gibbons Frederick X. , Ong Mei Ling , Gerrard Meg , Lei Man-Kit
Published: October 2022

In a sample of 685 late middle-aged Black adults (M age at 2019 = 57.17 years), we examined the effects of loneliness and per capita income on accelerated aging using a newly developed DNA-methylation based index: the DunedinPACE. First, using linear, mixed effects regression in a growth curve framework, we found that change in DunedinPACE was dependent on age, with a linear model best fitting the data (b = 0.004, p < 0.001), indicating that average pace of change increased among older participants. A quadratic effect was also tested, but was non-significant. Beyond the effect of age, both change in loneliness (b = 0.009, p < 0.05) and change in per capita income (b = −0.016, p < 0.001) were significantly associated with change in DunedinPACE across an 11-year period, accounting for significant between person variability observed in the unconditional model. Including non-self-report indices of smoking and alcohol use did not reduce the association of loneliness or per capita income with DunedinPACE. However, change in smoking was strongly associated with change in DunedinPACE such that those reducing their smoking aged less rapidly than those continuing to smoke. In addition, both loneliness and per capita income were associated with DunedinPACE after controlling for variation in cell-types.
The Analysis with Quantitative Indexes for Public’s Awareness of Radiation Knowledge in Taiwan
Authors: Feng Chen-Ju , Lai Yuan-Chun , Lee Shen-Hao , Lien Ke-Yu , Tseng Ching-Yu , Wu Ni-Shan , Liang Chiung-Ju , Wu Chin-Hui , Hsu Shih-Ming
Published: October 2022

(1) Background: The purpose of this study was to evaluate the radiation awareness level of the public in Taiwan. (2) Methods: This study designed an online survey form to investigate the radiation awareness level with six topics: basic knowledge of radiation, environmental radiation, medical radiation, radiation protection, and university/corporate social responsibility. The score of respondents were converted into knowledge and responsibility indexes for the quantitative evaluation. Logistic regression was used to assess the correlation between the knowledge index and individual factors. Paired t-test was used to assess the significant difference in knowledge index between pre-training and post-training. (3) Results: The knowledge index of each job category reflected the proportion of radiation awareness of the job. The logistic regression result indicated that radiation-related people could get higher knowledge index. The paired t-test indicated that the knowledge index before and after class had significant differences in all question topics. (4) Conclusions: The public’s awareness of medical radiation was the topic that needed to be strengthened the most—the responses with high knowledge index significantly correlated with their experience in radiation education training or radiation-related jobs. It significantly increased the knowledge index of radiation if the public received radiation education training.
The Impact of Workplace Violence on Headache and Sleep Problems in Nurses
Authors: Magnavita Nicola , Mele Luca , Meraglia Igor , Merella Marco , Vacca Maria Eugenia , Cerrina Anna , Gabriele Maddalena , Labella Marcella , Soro Maria Teresa , Ursino Simona , Matera Carmela
Published: October 2022

Workplace violence (WV) is a significant occupational hazard for nurses. Previous studies have shown that WV has a reciprocal relationship with occupational stress. Headaches and sleep problems are early neuropsychological signs of distress. This cross-sectional study aims to ascertain the frequency of physical or verbal assaults on nurses and to study the association of WV with headaches and sleep problems. During their regular medical examination in the workplace, 550 nurses and nursing assistants (105 males, 19.1%; mean age 48.02 ± 9.98 years) were asked to fill in a standardized questionnaire containing the Violent Incident Form (VIF) concerning the episodes of violence experienced, the Headache Impact Test (HIT-6) regarding headaches, and the Pittsburgh Sleep Quality Inventory (PSQI) on sleep quality. Occupational stress was measured using the Effort/Reward Imbalance questionnaire (ERI). Physical and non-physical violence experienced in the previous year was reported by 7.5% and 17.5% of workers, respectively. In the univariate logistic regression models, the workers who experienced violence had an increased risk of headaches and sleep problems. After adjusting for sex, age, job type, and ERI, the relationship between physical violence and headaches remained significant (adjusted odds ratio aOR = 2.25; confidence interval CI95% = 1.11; 4.57). All forms of WV were significantly associated with poor sleep in a multivariate logistic regression model adjusted for sex, age, job type, and ERI (aOR = 2.35 CI95% = 1.44; 3.85). WV was also associated with the impact of headaches and with sleep quality. WV prevention may reduce the frequency of lasting psychoneurological symptoms, such as headaches and poor sleep quality, that interfere with the ability to work.
Navigating the Storm to Recovery through the Pictorial Representations of Persons in the Recovery Phase from Unipolar Depression
Authors: Scerri Josianne , Bonnici Amy
Published: October 2022

Depression is a highly complex mental illness that presents challenges, such as difficulties for persons with depression to communicate their experiences. This is compounded further by a paucity of in-depth and pictorial accounts on their experiences of the recovery process. The combination of pictorial representations and interviews with persons who are recovering from depression, may assist them in communicating these lived experiences. Five participants recovering from unipolar depression and who were in the late stages of recovery were recruited through purposive sampling. Data were collected through the conduction of art sessions, where participants pictorially represented their experience of living with depression and their road to recovery. Semi-structured interviews were then used to explore their artwork. The transcripts were analysed using Interpretative Phenomenological Analysis (IPA). Two superordinate themes emerged from participants’ interviews, namely: ‘A New Me in Me’ that incorporating changes in their identity, physical, emotional, and social experiences, and ‘Life as an amalgamation of colour’ describing their search for meaning and the importance of spirituality, hope, gaining control and positivity in the recovery process. The use of pictorial representations combined with interviews can add depth to participant narratives, that serve to enhance the therapeutic alliance between the patient–professional dyad.
Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil
Authors: Arenas Francisco , Morte Asunción , Navarro-Ródenas Alfonso
Published: October 2022

Desert truffle crop is a pioneer in southeastern Spain, a region where native edible hypogeous fungi are adapted to the semiarid areas with low annual rainfall. Terfezia claveryi Chatin was the first species of desert truffle to be cultivated, and has been increasing in recent years as an alternative rainfed crop in the Iberian Peninsula. However, its behaviour in the field has yet not been investigated. For this purpose, specific primers were designed for the soil DNA quantification of both T. claveryi and Terfezia crassiverrucosa and a real-time qPCR protocol was developed, using the ITS rDNA region as a target. Moreover, a young desert truffle orchard was sampled for environmental validation. The results showed the highest efficiency for the TerclaF3/TerclaR1 primers pair, 89%, and the minimal fungal biomass that could be reliable detected was set at 4.23 µg mycelium/g soil. The spatial distribution of fungal biomass was heterogeneous, and there was not a direct relationship between the quantity of winter soil mycelium and the location/productivity of desert truffles. This protocol could be applied to tracking these species in soil and understand their mycelial dynamics in plantations and wild areas.
Algorithm for Designing a Removable Complete Denture (RCD) Based on the FEM Analysis of Its Service Life
Authors: Grachev Dmitry I. , Ruzuddinov Nurmukhamet S. , Arutyunov Anatoliy S. , Akhmedov Gadzhi D. , Dubova Lubov V. , Kharakh Yaser N. , Panin Sergey V. , Arutyunov Sergey D.
Published: October 2022

(1) Background: The paper addresses the computer simulation and prediction of the service life of the base of removable complete dentures (RCDs) under typical loads caused by biting and chewing food. For this purpose, the finite element method (FEM) was used. It is assumed that various blocks of teeth, such as incisors, canines, premolars and molars, are subjected to cyclic impacts during a human life. (2) Methods: Both symmetric and asymmetric mastication (two- and one-sided loads, respectively) cases were considered. The load level was assumed to be 100 N, which corresponds to the average muscular compression force of typical human jaws. (3) Results: The FEM analysis of the stress–strain state evolution for RCDs under cyclic loads was carried out. Maps of equivalent lines were drawn for the denture base in terms of its durability. A multi-axial criterion was implemented to determine the number of cycles prior to failure by the mechanism of a normal opening mode crack. The FEM-based assessment of the service life of RCDs enabled us to establish the critical stress concentration areas, thereby allowing for further planning for the correction of an occlusal scheme or teeth inclinations. As a result, the service life of RCDs under cyclic loading can be improved. (4) Conclusions: An algorithm for designing RCDs in the case of edentulism based on the FEM simulation using commercial software as part of the procedure is proposed.
Synthesis of Bimetallic FeCu-MOF and Its Performance as Catalyst of Peroxymonosulfate for Degradation of Methylene Blue
Authors: Li Huanxuan , Xu Chen , Li Ning , Rao Tao , Zhou Zhong , Zhou Qingwei , Wang Chunhui , Xu Shaodan , Tang Junhong
Published: October 2022

Bimetallic MOFs have recently emerged as promising materials for wastewater treatment based on advanced oxidation processes. Herein, a new bimetallic MOF (FeCu-MOF) was fabricated by hydrothermal process. The structural, morphological, compositional and physicochemical properties of the as-synthesized bimetallic FeCu-MOF were characterized by XRD, FT-IR, SEM, TEM, BET, and XPS. TEM and XPS confirmed the homogeneous distribution of CuO2 nanoparticles in the as-synthesized materials. The result of wastewater treatment indicated that 100% of MB was removed by 6.0 mM PMS activated with 0.6 g/L of FeCu-MOF in 30 min. The high catalytic performance of FeCu-MOF was probably due to the accelerated electron and mass transfer resulting from the existence of a homogeneous distribution of unsaturated metal sites and an abundant mesoporous structure. The obtained results from the competitive quenching tests demonstrated that sulfate radicals (SO4•−) were the major species responsible for MB oxidation. In addition, hydroxyl (·OH) and singlet oxygen (1O2) also had a nonnegligible role in the MB removal. Interestingly, the addition of acetate ion (CHCOO−) promoted the removal of MB while other anions (including NO2−, H2PO4−, SO42−, HPO42−, and HCO3−) inhibited the MB removal. Furthermore, a possible mechanism based on both heterogeneous and homogeneous activation of PMS was proposed, along with the MB degradation mechanism.
Nonlocal Probability Theory: General Fractional Calculus Approach
Authors: Tarasov Vasily E.
Published: October 2022

Nonlocal generalization of the standard (classical) probability theory of a continuous distribution on a positive semi-axis is proposed. An approach to the formulation of a nonlocal generalization of the standard probability theory based on the use of the general fractional calculus in the Luchko form is proposed. Some basic concepts of the nonlocal probability theory are proposed, including nonlocal (general fractional) generalizations of probability density, cumulative distribution functions, probability, average values, and characteristic functions. Nonlocality is described by the pairs of Sonin kernels that belong to the Luchko set. Properties of the general fractional probability density function and the general fractional cumulative distribution function are described. The truncated GF probability density function, truncated GF cumulative distribution function, and truncated GF average values are defined. Examples of the general fractional (GF) probability distributions, the corresponding probability density functions, and cumulative distribution functions are described. Nonlocal (general fractional) distributions are described, including generalizations of uniform, degenerate, and exponential type distributions; distributions with the Mittag-Leffler, power law, Prabhakar, Kilbas–Saigo functions; and distributions that are described as convolutions of the operator kernels and standard probability density.
Static Recrystallization Behavior of Low-Carbon Nb-V-Microalloyed Forging Steel
Authors: Zhao Yang , Zheng Jiahao , Chen Liqing , Liu Xianghua
Published: October 2022

Static recrystallization is a method of tailoring the microstructure and mechanical properties of steels, which is important for microalloyed forging steels as the hot deformation process significantly affects their mechanical properties. In this paper, the static recrystallization behavior of a low-carbon Nb-V-microalloyed forging steel was investigated by double-pass hot compression tests at deformation temperature of 800–1100 °C and interruption time of 1–1000 s. The static recrystallization fractions were determined using the 2% offset method. The static recrystallization activation energy and the static recrystallization critical temperature (SRCT) of the experimental steel were determined. When the deformation temperature was higher than the SRCT, the recrystallization fraction curve conformed to the Avrami equation. When the deformation temperature was below the SRCT, the recrystallization curve appeared to plateau, which was caused by strain-induced precipitation. Before and after the plateau, the static recrystallization kinetics still obeyed the Avrami equation.
Identification of Exhaled Metabolites in Children with Cystic Fibrosis
Authors: Weber Ronja , Perkins Nathan , Bruderer Tobias , Micic Srdjan , Moeller Alexander
Published: October 2022

The early detection of inflammation and infection is important to prevent irreversible lung damage in cystic fibrosis. Novel and non-invasive monitoring tools would be of high benefit for the quality of life of patients. Our group previously detected over 100 exhaled mass-to-charge (m/z) features, using on-line secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS), which distinguish children with cystic fibrosis from healthy controls. The aim of this study was to annotate as many m/z features as possible with putative chemical structures. Compound identification was performed by applying a rigorous workflow, which included the analysis of on-line MS2 spectra and a literature comparison. A total of 49 discriminatory exhaled compounds were putatively identified. A group of compounds including glycolic acid, glyceric acid and xanthine were elevated in the cystic fibrosis group. A large group of acylcarnitines and aldehydes were found to be decreased in cystic fibrosis. The proposed compound identification workflow was used to identify signatures of volatile organic compounds that discriminate children with cystic fibrosis from healthy controls, which is the first step for future non-invasive and personalized applications.
Actuators for Implantable Devices: A Broad View
Authors: Yan Bingxi
Published: October 2022

The choice of actuators dictates how an implantable biomedical device moves. Specifically, the concept of implantable robots consists of the three pillars: actuators, sensors, and powering. Robotic devices that require active motion are driven by a biocompatible actuator. Depending on the actuating mechanism, different types of actuators vary remarkably in strain/stress output, frequency, power consumption, and durability. Most reviews to date focus on specific type of actuating mechanism (electric, photonic, electrothermal, etc.) for biomedical applications. With a rapidly expanding library of novel actuators, however, the granular boundaries between subcategories turns the selection of actuators a laborious task, which can be particularly time-consuming to those unfamiliar with actuation. To offer a broad view, this study (1) showcases the recent advances in various types of actuating technologies that can be potentially implemented in vivo, (2) outlines technical advantages and the limitations of each type, and (3) provides use-specific suggestions on actuator choice for applications such as drug delivery, cardiovascular, and endoscopy implants.
Editorial for Special Issue “Modeling, Design, and Optimization of Multiphase Systems in Minerals Processing, Volume II”
Authors: Lucay Freddy A. , Cisternas Luis A.
Published: October 2022

The manuscripts published in the 2019 Special Issue “Modeling, Design, and Optimization of Multiphase Systems in Minerals Processing” [...]
Application of Curcumin Emulsion Carrier from Ultrasonic-Assisted Prepared Octenyl Succinic Anhydride Rice Starch
Authors: Zheng Yuxue , Zhang Huiling , Wei Xiaobo , Fang Haitian , Tian Jinhu
Published: October 2022

The emulsification of ultrasonic-assisted prepared octenyl succinic anhydride (OSA) rice starch on curcumin was investigated in the present study. The results indicated that the encapsulation efficiency of curcumin in emulsions stabilized by OSA-ultrasonic treatment rice starch was improved, from 81.65 ± 0.14% to 89.03 ± 0.09%. During the in vitro oral digestion, the particle size and Zeta potential of the curcumin emulsion did not change significantly (p > 0.05). During the in vitro digestive stage of the stomach and small intestine, the particle size of the curcumin emulsion continued to increase, and the absolute potential continued to decrease. Our work showed that OSA-pre-treatment ultrasonic rice starch could improve curcumin bioavailability by increasing the encapsulation efficiency with stronger stability to avoid the attack of enzymes and high intensity ion, providing a way to develop new emulsion-based delivery systems for bioactive lipophilic compounds using OSA starch.
Random Copolymers of Styrene with Pendant Fluorophore Moieties: Synthesis and Applications as Fluorescence Sensors for Nitroaromatics
Authors: Zen Eddin Mohamad , Zhilina Ekaterina F. , Chuvashov Roman D. , Dubovik Alyona I. , Mekhaev Alexandr V. , Chistyakov Konstantin A. , Baranova Anna A. , Khokhlov Konstantin O. , Rusinov Gennady L. , Verbitskiy Egor V. , Charushin Valery N.
Published: October 2022

Five random copolymers comprising styrene and styrene with pendant fluorophore moieties, namely pyrene, naphthalene, phenanthrene, and triphenylamine, in molar ratios of 10:1, were synthesized and employed as fluorescent sensors. Their photophysical properties were investigated using absorption and emission spectral analyses in dichloromethane solution and in solid state. All copolymers possessed relative quantum yields up to 0.3 in solution and absolute quantum yields up to 0.93 in solid state, depending on their fluorophore components. Fluorescence studies showed that the emission of these copolymers is highly sensitive towards various nitroaromatic compounds, both in solution and in the vapor phase. The detection limits of these fluorophores for nitroaromatic compounds in dichloromethane solution proved to be in the range of 10−6 to 10−7 mol/L. The sensor materials for new hand-made sniffers based on these fluorophores were prepared by electrospinning and applied for the reliable detection of nitrobenzene vapors at 1 ppm in less than 5 min.
Transcriptomics and Proteomics Characterizing the Anticancer Mechanisms of Natural Rebeccamycin Analog Loonamycin in Breast Cancer Cells
Authors: Sun Xiao , Lu Zhanying , Liang Zhenzhen , Deng Bowen , Zhu Yuping , Shi Jing , Lu Xiaoling
Published: October 2022

The present study is to explore the anticancer effect of loonamycin (LM) in vitro and in vivo, and investigate the underlying mechanism with combined multi-omics. LM exhibited anticancer activity in human triple negative breast cancer cells by promoting cell apoptosis. LM administration inhibited the growth of MDA-MB-468 tumors in a murine xenograft model of breast cancer. Mechanistic studies suggested that LM could inhibit the topoisomerase I in a dose-dependent manner in vitro experiments. Combined with the transcriptomics and proteomic analysis, LM has a significant effect on O-glycan, p53-related signal pathway and EGFR/PI3K/AKT/mTOR signal pathway in enrichment of the KEGG pathway. The GSEA data also suggests that the TNBC cells treated with LM may be regulated by p53, O-glycan and EGFR/PI3K/AKT/mTOR signaling pathway. Taken together, our findings predicted that LM may target p53 and EGFR/PI3K/AKT/mTOR signaling pathway, inhibiting topoisomerase to exhibit its anticancer effect.
Trapping of Ag+ into a Perfect Six-Coordinated Environment: Structural Analysis, Quantum Chemical Calculations and Electrochemistry
Authors: Komlyagina Veronika I. , Romashev Nikolay F. , Kokovkin Vasily V. , Gushchin Artem L. , Benassi Enrico , Sokolov Maxim N. , Abramov Pavel A.
Published: October 2022

Self-assembly of (Bu4N)4[β-Mo8O26], AgNO3, and 2-bis[(2,6-diisopropylphenyl)-imino]acenaphthene (dpp-bian) in DMF solution resulted in the (Bu4N)2[β-{Ag(dpp-bian)}2Mo8O26] (1) complex. The complex was characterized by single crystal X-ray diffraction (SCXRD), X-ray powder diffraction (XRPD), diffuse reflectance (DR), infrared spectroscopy (IR), and elemental analysis. Comprehensive SCXRD studies of the crystal structure show the presence of Ag+ in an uncommon coordination environment without a clear preference for Ag-N over Ag-O bonding. Quantum chemical calculations were performed to qualify the nature of the Ag-N/Ag-O interactions and to assign the electronic transitions observed in the UV–Vis absorption spectra. The electrochemical behavior of the complex combines POM and redox ligand signatures. Complex 1 demonstrates catalytic activity in the electrochemical reduction of CO2.
Phytochemical Analysis and Understanding the Antioxidant and Anticancer Properties of Methanol Extract from Litsea glutinosa: In Vitro and In Vivo Studies
Authors: Shafiq Shafia , Zahan Ronok , Yesmin Samina , Khan Alam , Mahmud Md. Sabbir , Reza Md Abu , Albogami Sarah M. , Alorabi Mohammed , De Waard Michel , Saad Hebatallah M. , Sabatier Jean-Marc , Naz Tarannum , Batiha Gaber El-Saber
Published: October 2022

Litsea glutinosa (L. glutinosa) is considered an evidence-based medicinal plant for the treatment of cancer, the leading cause of death worldwide. In our study, the in vitro antioxidant and in vivo anticancer properties of an essential ethno-medicinal plant, L. glutinosa, were examined using non-toxic doses and a phytochemical analysis was executed using gas-chromatography–mass-spectrometry. The in vitro antioxidant study of the L. glutinosa methanolic extract (LGBME) revealed a concentration-dependent antioxidant property. The bark extract showed promising antioxidant effects in the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. The strongest antioxidant activity was demonstrated at the maximum concentration (50 µg/mL). The IC50 values of the LGBME and BHT were 5.51 and 5.01 µg/mL, respectively. At the same concentration, the total antioxidant capacity of the LGBME was 0.161 µg/mL and the ferric reducing antioxidant power assay result of the LGBME was 1.783 µg/mL. In the cytotoxicity study, the LD50 of the LGBME and gallic acid were 24.93 µg/mL and 7.23 µg/mL, respectively. In the in vivo anticancer-activity studies, the LGBME, particularly at a dose of 150 mg/kg/bw, showed significant cell-growth inhibition, decreased tumor weight, increased mean survival rate, and upregulated the reduced hematological parameters in EAC (Ehrlich’s ascites carcinoma)-induced Swiss albino mice. The highest cell-growth inhibition, 85.76%, was observed with the dose of 150 mg/kg/bw. Furthermore, the upregulation of pro-apoptotic genes (p53, Bax) and the downregulation of anti-apoptotic Bcl-2 were observed. In conclusion, LGBME extract has several bioactive phytoconstituents, which confirms the antioxidant and anticancer properties of L. glutinosa.
Mechanism, Kinetics and Thermodynamics of Decomposition for High Energy Derivatives of [1,2,4]Triazolo[4,3-b][1,2,4,5]tetrazine
Authors: Stankevich Aleksandr V. , Tolshchina Svetlana G. , Korotina Anna V. , Rusinov Gennady L. , Chemagina Irina V. , Charushin Valery N.
Published: October 2022

This paper presents the data of research studies on the mechanisms, kinetics and thermodynamics of decomposition of three high-energy compounds: [1,2,4]triazolo[4,3-b][1,2,4,5]tetrazine-3,6-diamine (TTDA), 3-amino-6-hydrazino[1,2,4]triazolo[4,3-b][1,2,4,5]tetrazine (TTGA) and 3,6-dinitroamino[1,2,4]triazolo[4,3-b][1,2,4,5]tetrazine (DNTT). The points of change of the reaction mechanisms under thermal effects with different intensities from 0.1 to 2000 s−1 have been established. The values of activation and induction energies for the limiting stages of decomposition have been obtained. The formation of nanostructured carbon nitride (α-C3N4) in condensed decomposition products, cyanogen (C2N2) and hydrogen cyanide (HCN) in gaseous products have been shown. Concentration-energy diagrams for the reaction products have been compiled. The parameters of heat resistance and thermal safety proved to be: 349.5 °C and 358.2 °C for TTDA; 190.3 °C and 198.0 °C for TTGA; 113.4 °C and 114.1 °C for DNTT. The energy and thermodynamic properties have also been estimated. This work found the activation energy of the decomposition process to be 129.0 kJ/mol for TTDA, 212.2 kJ/mol for TTGA and 292.2 kJ/mol for DNTT. The average induction energy of the catalytic process (Ecat) for TTGA was established to be 21 kJ/mol, and for DNTT-1500–1700 kJ/mol. The induction energy of the inhibition process (Eing) of TTDA was estimated to be 800–1400 kJ/mol.
Application of Deep-Learning Algorithm Driven Intelligent Raman Spectroscopy Methodology to Quality Control in the Manufacturing Process of Guanxinning Tablets
Authors: Tao Yi , Bao Jiaqi , Liu Qing , Liu Li , Zhu Jieqiang
Published: October 2022

Coupled with the convolutional neural network (CNN), an intelligent Raman spectroscopy methodology for rapid quantitative analysis of four pharmacodynamic substances and soluble solid in the manufacture process of Guanxinning tablets was established. Raman spectra of 330 real samples were collected by a portable Raman spectrometer. The contents of danshensu, ferulic acid, rosmarinic acid, and salvianolic acid B were determined with high-performance liquid chromatography-diode array detection (HPLC-DAD), while the content of soluble solid was determined by using an oven-drying method. In the establishing of the CNN calibration model, the spectral characteristic bands were screened out by a competitive adaptive reweighted sampling (CARS) algorithm. The performance of the CNN model is evaluated by root mean square error of calibration (RMSEC), root mean square error of cross-validation (RMSECV), root mean square error of prediction (RMSEP), coefficient of determination of calibration (Rc2), coefficient of determination of cross-validation (Rcv2), and coefficient of determination of validation (Rp2). The Rp2 values for soluble solid, salvianolic acid B, danshensu, ferulic acid, and rosmarinic acid are 0.9415, 0.9246, 0.8458, 0.8667, and 0.8491, respectively. The established model was used for the analysis of three batches of unknown samples from the manufacturing process of Guanxinning tablets. As the results show, Raman spectroscopy is faster and more convenient than that of conventional methods, which is helpful for the implementation of process analysis technology (PAT) in the manufacturing process of Guanxinning tablets.
Composite Nanofibers as Novel Sorbents for On-Line and Off-Line Solid-Phase Extraction in Chromatographic System: A Comparison for Detection of Free Biogenic Monoamines and Their Metabolites in Plasma
Authors: Chen Liqin , Bi Yueling , Xu Tong , Li Xiaohuan , Fang Zhongze
Published: October 2022

Two different pretreatment approaches have been used for the enrichment and separation of biogenic monoamines and metabolites in plasma for high performance liquid chromatography (HPLC) determination. The first approach, based on on-line packed-fiber solid-phase extraction (PFSPE) coupled with HPLC, allows for the simultaneous detection of epinephrine (E), norepinephrine (NE), dopamine (DA), 3-methoxyl epinephrine (MN), norepinephrine (NMN), 3-methoxytyramine (3-MT), and 5-hydroxytryptamin (5-HT). Using this developed on-line PFSPE–HPLC method, the limit of detections (LODs) of the seven analytes ranged from 1 ng/mL (NMN and MN) to 2 ng/mL (NE, E, DA, 3-MT and 5-HT). The reportable ranges were 5–300 ng/mL for NE and DA, 5–100 ng/mL for E, and 5–200 ng/mL for NMN, MN, 3-MT and 5-HT. The off-line PFSPE–HPLC was employed in the second approach and could provide simultaneous detection of NE, E, DA, NMN, and MN. The linearity was verified in the range of 0.5–20 ng/mL (NE, E, and DA) and 20–250 ng/mL (NMN and MN). The LODs of the five analytes ranged from 0.2 ng/mL (NE, E, and DA) to 5 ng/mL (NMN and MN). This study verified the possibility of using nanofibers as an adsorbent in an on-line PFSPE–HPLC system for the determination of biogenic monoamines and their metabolites in human plasma. Compared with the off-line PFSPE approach, the on-line PFSPE method deserves attention mainly due to its greener character, derived from the automation of the process and high-throughput with less operators’ handling.
Effect of Currently Available Nanoparticle Synthesis Routes on Their Biocompatibility with Fibroblast Cell Lines
Authors: Mansoor Afsheen , Khurshid Zohaib , Mansoor Emaan , Khan Muhammad Talal , Ratnayake Jithendra , Jamal Asif
Published: October 2022

Nanotechnology has acquired significance in dental applications, but its safety regarding human health is still questionable due to the chemicals utilized during various synthesis procedures. Titanium nanoparticles were produced by three novel routes, including Bacillus subtilis, Cassia fistula and hydrothermal heating, and then characterized for shape, phase state, size, surface roughness, elemental composition, texture and morphology by SEM, TEM, XRD, AFM, DRS, DLS and FTIR. These novel titanium nanoparticles were tested for cytotoxicity through the MTT assay. L929 mouse fibroblast cells were used to test the cytotoxicity of the prepared titanium nanoparticles. Cell suspension of 10% DMEM with 1 × 104 cells was seeded in a 96-well plate and incubated. Titanium nanoparticles were used in a 1 mg/mL concentration. Control (water) and titanium nanoparticles stock solutions were prepared with 28 microliters of MTT dye and poured into each well, incubated at 37 °C for 2 h. Readings were recorded on day 1, day 15, day 31, day 41 and day 51. The results concluded that titanium nanoparticles produced by Bacillus subtilis remained non-cytotoxic because cell viability was >90%. Titanium nanoparticles produced by Cassia fistula revealed mild cytotoxicity on day 1, day 15 and day 31 because cell viability was 60–90%, while moderate cytotoxicity was found at day 41 and day 51, as cell viability was 30–60%. Titanium nanoparticles produced by hydrothermal heating depicted mild cytotoxicity on day 1 and day 15; moderate cytotoxicity on day 31; and severe cytotoxicity on day 41 and day 51 because cell viability was less than 30% (p < 0.001). The current study concluded that novel titanium nanoparticles prepared by Bacillus subtilis were the safest, more sustainable and most biocompatible for future restorative nano-dentistry purposes.