bluuebunny/crossref_metadata_2025 huggingface dataset:
Self-Ridiculing Nostalgia: Joseph Roth’sDie Kapuzinergruft
Authors: Heady Katy
Published: February 2018

This article argues for a reinterpretation of the role of Habsburg nostalgia in Die Kapuzinergruft, the final novel by the Austrian-Jewish writer Joseph Roth. From the early 1930s onward, Roth had been an enthusiastic proponent of the Habsburg myth, and the tenor of his pro-Habsburg journalism became particularly adulatory during the final years of his life. Partly as a result of this, Die Kapuzinergruft is frequently read as a heavy-handed endorsement of Austria’s imperial past, although several valuable contra-nostalgic interpretations of the text have appeared in recent years. This article reconciles the differences between these two views of the novel by arguing that in fact the work dramatizes Roth’s inner conflict between the solace offered by Habsburg nostalgia and his own awareness of the perceptual, psychological, and moral weaknesses that such nostalgia entailed.
Design Optimization of Steering Mechanisms for Articulated Off-Road Vehicles Based on Genetic Algorithms
Authors: Zhou Chen , Liu Xinhui , Xu Feixiang
Published: February 2018

Two cylinders arranged symmetrically on a frame have become a major form of steering mechanism for articulated off-road vehicles (AORVs). However, the differences of stroke and arm lead to pressure fluctuation, vibration noise, and a waste of torque. In this paper, the differences of stroke and arm are reduced based on a genetic algorithm (GA). First, the mathematical model of the steering mechanism is put forward. Then, the difference of stroke and arm are optimized using a GA. Finally, a FW50GLwheel loader is used as an example to demonstrate the proposed GA-based optimization method, and its effectiveness is verified by means of automatic dynamic analysis of mechanical systems (ADAMS). The stroke difference of the steering hydraulic cylinders was reduced by 92% and the arm difference reached a decrease of 78% through GA optimization, in comparison with unoptimized structures. The simulation result shows that the steering mechanism optimized by GA behaved better than by previous methods.
Diversification of Secondary Metabolite Biosynthetic Gene Clusters Coincides with Lineage Divergence in Streptomyces
Authors: Choudoir Mallory , Pepe-Ranney Charles , Buckley Daniel
Published: February 2018

We have identified Streptomyces sister-taxa which share a recent common ancestor and nearly identical small subunit (SSU) rRNA gene sequences, but inhabit distinct geographic ranges demarcated by latitude and have sufficient genomic divergence to represent distinct species. Here, we explore the evolutionary dynamics of secondary metabolite biosynthetic gene clusters (SMGCs) following lineage divergence of these sister-taxa. These sister-taxa strains contained 310 distinct SMGCs belonging to 22 different gene cluster classes. While there was broad conservation of these 22 gene cluster classes among the genomes analyzed, each individual genome harbored a different number of gene clusters within each class. A total of nine SMGCs were conserved across nearly all strains, but the majority (57%) of SMGCs were strain-specific. We show that while each individual genome has a unique combination of SMGCs, this diversity displays lineage-level modularity. Overall, the northern-derived (NDR) clade had more SMGCs than the southern-derived (SDR) clade (40.7 ± 3.9 and 33.8 ± 3.9, mean and S.D., respectively). This difference in SMGC content corresponded with differences in the number of predicted open reading frames (ORFs) per genome (7775 ± 196 and 7093 ± 205, mean and S.D., respectively) such that the ratio of SMGC:ORF did not differ between sister-taxa genomes. We show that changes in SMGC diversity between the sister-taxa were driven primarily by gene acquisition and deletion events, and these changes were associated with an overall change in genome size which accompanied lineage divergence.
Efficacy of an Optimised Bacteriophage Cocktail to Clear Clostridium difficile in a Batch Fermentation Model
Authors: Nale Janet , Redgwell Tamsin , Millard Andrew , Clokie Martha
Published: February 2018

Clostridium difficile infection (CDI) is a major cause of infectious diarrhea. Conventional antibiotics are not universally effective for all ribotypes, and can trigger dysbiosis, resistance and recurrent infection. Thus, novel therapeutics are needed to replace and/or supplement the current antibiotics. Here, we describe the activity of an optimised 4-phage cocktail to clear cultures of a clinical ribotype 014/020 strain in fermentation vessels spiked with combined fecal slurries from four healthy volunteers. After 5 h, we observed ~6-log reductions in C. difficile abundance in the prophylaxis regimen and complete C. difficile eradication after 24 h following prophylactic or remedial regimens. Viability assays revealed that commensal enterococci, bifidobacteria, lactobacilli, total anaerobes, and enterobacteria were not affected by either regimens, but a ~2-log increase in the enterobacteria, lactobacilli, and total anaerobe abundance was seen in the phage-only-treated vessel compared to other treatments. The impact of the phage treatments on components of the microbiota was further assayed using metagenomic analysis. Together, our data supports the therapeutic application of our optimised phage cocktail to treat CDI. Also, the increase in specific commensals observed in the phage-treated control could prevent further colonisation of C. difficile, and thus provide protection from infection being able to establish.
A Proposal about the Meaning of Scale, Scope and Resolution in the Context of the Information Interpretation Process
Authors: Febres Gerardo
Published: February 2018

When considering perceptions, the observation scale and resolution are closely related properties. There is consensus on considering resolution as the density of the elementary pieces of information in a specified information space. On the other hand, with the concept of scale, several conceptions compete for a consistent meaning. Scale is typically regarded as a way to indicate the degree of detail in which an observation is performed. Surprisingly, there is not a unified definition of scale as a description’s property. This paper offers a precise definition of scale and a method to quantify it as a property associated with the interpretation of a description. To complete the parameters needed to describe the perception of a description, the concepts of scope and resolution are also revealed with an exact meaning. A model describing a recursive process of interpretation, based on evolving steps of scale, scope and resolution, is introduced. The model relies on the conception of observation scale and its association to the selection of symbols. Five experiments illustrate the application of these concepts, showing that resolution, scale and scope integrate the set of properties to define any point of view from which an observation is performed and interpreted. The results obtained for descriptions expressed in one and two dimensions, are the basis for a comparison of the perceivable symbolic information from different interpretations of the same descriptions. In conclusion, this study provides a framework for building models of our interpretation process and suggests ways to understand some mechanisms in the formation of information from initially meaningless symbols.
Ultrasensitive Determination of Malathion Using Acetylcholinesterase Immobilized on Chitosan-Functionalized Magnetic Iron Nanoparticles
Authors: Rodrigues Núbia , Neto Sakae , Luz Rita , Damos Flávio , Yamanaka Hideko
Published: February 2018

A renewable, disposable, low cost, and sensitive sensor for the detection of organophosphorus pesticides was constructed by immobilizing the acetylcholinesterase enzyme (AChE), via glutaraldehyde, on magnetic iron nanoparticles (Fe3O4) previously synthesized and functionalized with chitosan (CS). The sensor was denoted AChE/CS/Fe3O4. The magnetic nanoparticles were characterized by Fourier transform infrared spectroscopy and transmission electron microscopy. Acetylthiocholine (ATCh) was incubated with AChE/CS/Fe3O4 and attached to a screen-printed electrode using a magnet. The oxidation of thiocholine (from ATCh hydrolysis) was monitored at an applied potential of +0.5 V vs. Ag/AgCl(KClsat) in 0.1 mol L−1 phosphate buffer solution (pH 7.5) as the supporting electrolyte. A mixture of the pesticide malathion and ATCh was investigated using the same procedure, and the results were compared and expressed as inhibition percentages. For determination of malathion, the proposed sensor presented a linear response in the range from 0.5 to 20 nmol L−1 (R = 0.9942). The limits of detection (LOD) and quantification (LOQ) were 0.3 and 0.8 nmol L−1, respectively. Real samples were also investigated, with recovery values of 96.0% and 108.3% obtained for tomato and pond water samples, respectively. The proposed sensor is a feasible option for malathion detection, offering a linear response, good sensitivity, and a low detection limit.
Heterogeneous Catalysis by Tetraethylammonium Tetrachloroferrate of the Photooxidation of Toluene by Visible and Near-UV Light
Authors: Barnard Kelsie , Bright Valerie , Enright Robert , Fahy Kira , Liu Adam , Hoggard Patrick
Published: February 2018

Titanium dioxide is the most extensively used heterogeneous catalyst for the photooxidation of toluene and other hydrocarbons, but it has low utility for the synthesis of benzyl alcohol, of which little is produced, or benzaldehyde, due to further oxidation to benzoic acid and cresol, among other oxidation products, and eventually complete mineralization to CO2. Et4N[FeCl4] functions as a photocatalyst through the dissociation of chlorine atoms, which abstract hydrogen from toluene, and the photooxidation of toluene proceeds only as far as benzyl alcohol and benzaldehyde. Unlike TiO2, which requires ultraviolet (UV) irradiation, Et4N[FeCl4] catalyzes the photooxidation of toluene with visible light alone. Even under predominantly UV irradiation, the yield of benzyl alcohol plus benzaldehyde is greater with Et4N[FeCl4] than with TiO2. Et4N[FeCl4] photocatalysis yields benzyl chloride as a side product, but it can be minimized by restricting irradiation to wavelengths above 360 nm and by the use of long irradiation times. The photonic efficiency of oxidation in one experiment was found to be 0.042 mol/einstein at 365 nm. The use of sunlight as the irradiation source was explored.
Two-Liquid Flotation for Separating Mixtures of Ultra-Fine Rare Earth Fluorescent Powders for Material Recycling—A Review
Authors: Otsuki Akira , Dodbiba Gjergj , Fujita Toyohisa
Published: February 2018

This paper reviews two separation methods applying two-step two-liquid flotation for recovering ultra-fine rare earth fluorescent powders (i.e., red, green, and blue). The paper aims to extract the science behind separation by two-liquid flotation, and to provide resulting engineering tips for material recycling. Two-liquid flotation, also called liquid-liquid extraction, involves two solvents (i.e., non-polar and polar solvents) to capture hydrophobic/hydrophobized particles at their interface, and a surfactant to selectively modify the surface property of the target powder(s). For separating a three powder mixture, two different developed flowsheets, composed of two-step separation are discussed. The major difference found was the polar solvents used. The first flowsheet (called the aqueous-organic system) employed water as a polar solvent while the second flowsheet (called the organic-organic system) utilized N,N-dimethylformamide, DMF as a polar solvent. The organic-organic system at the optimized conditions achieved both the grade and recovery of all the separated fluorescent powders at greater than 90% while the aqueous-organic system did not satisfy these criteria. This paper also reviews the mechanism behind the separation, as well as performing a cost comparison between the two methods. The cost comparison indicates that the organic-organic system is a more cost effective method for recovering rare earth fluorescent powders than the aqueous-organic system. Since the size of powders (i.e., several µm) is too small for the application of conventional separation technologies (e.g., froth flotation), two-liquid flotation is a unique pathway for the material recycling of ultra-fine rare earth fluorescent powders.
UAS Navigation with SqueezePoseNet—Accuracy Boosting for Pose Regression by Data Augmentation
Authors: Mueller Markus , Jutzi Boris
Published: February 2018

The navigation of Unmanned Aerial Vehicles (UAVs) nowadays is mostly based on Global Navigation Satellite Systems (GNSSs). Drawbacks of satellite-based navigation are failures caused by occlusions or multi-path interferences. Therefore, alternative methods have been developed in recent years. Visual navigation methods such as Visual Odometry (VO) or visual Simultaneous Localization and Mapping (SLAM) aid global navigation solutions by closing trajectory gaps or performing loop closures. However, if the trajectory estimation is interrupted or not available, a re-localization is mandatory. Furthermore, the latest research has shown promising results on pose regression in 6 Degrees of Freedom (DoF) based on Convolutional Neural Networks (CNNs). Additionally, existing navigation methods can benefit from these networks. In this article, a method for GNSS-free and fast image-based pose regression by utilizing a small Convolutional Neural Network is presented. Therefore, a small CNN (SqueezePoseNet) is utilized, transfer learning is applied and the network is tuned for pose regression. Furthermore, recent drawbacks are overcome by applying data augmentation on a training dataset utilizing simulated images. Experiments with small CNNs show promising results for GNSS-free and fast localization compared to larger networks. By training a CNN with an extended data set including simulated images, the accuracy on pose regression is improved up to 61.7% for position and up to 76.0% for rotation compared to training on a standard not-augmented data set.
Relationships between Wood Formation and Cambium Phenology on the Tibetan Plateau during 1960–2014
Authors: He Minhui , Yang Bao , Shishov Vladimir , Rossi Sergio , Bräuning Achim , Ljungqvist Fredrik , Grießinger Jussi
Published: February 2018

The variability of tree stem phenology plays a critical role in determining the productivity of forest ecosystems. Therefore, we aim to identify the relationships between the timings of cambium phenology, and forest growth in terms of tree-ring width over a long-term scale. A meta-analysis was performed that combined the timings of xylem formation, which were calculated by a tree-ring formation model of the VS (Vaganov-Shashkin)-oscilloscope during the period 1960–2014, and a tree-ring width series at 20 composite sites on the Tibetan Plateau. Both the start and length of the growing season significantly affected the formation of wood at 70% of the 20 composite sites within the study region. A wider tree ring probably resulted from an earlier start and a longer duration of the growing season. The influence of ending dates on tree-ring width was less evident, and more site-dependent. Weak relationships were identified between the start and end of the growing season at 85% of the composite sites. Compared to the monitoring results, which could only detect the relationships between cambium phenology and xylem cell production from a limited number of trees and years, our long-term relationships deepened such connections, and therefore should be used to improve mechanism models for the accurate evaluating and predicting of wood production and carbon sequestration in forest ecosystems under current and future climate change.
Transcriptome Analysis of the Biosynthesis of Anthocyanins in Begonia semperflorens under Low-Temperature and High-Light Conditions
Authors: Dong Yanpeng , Qu Ying , Qi Rui , Bai Xue , Tian Geng , Wang Yang , Wang Jiawan , Zhang Kaiming
Published: February 2018

Anthocyanins are considered a stress indicator due to their involvement in the response to many stresses including high light (HL) and low temperature (LT). With the development of transcriptomics, it is necessary to find the different and common points in the mechanisms of LT-induced and HL-induced anthocyanin biosynthesis. In the present study, we determined the transcriptomes of Begonia semperflorens leaves under three different conditions (normal growing conditions (CK), HL, and LT). To validate the differentially expressed genes (DEGs), we selected four core genes involved in anthocyanin biosynthesis to perform real-time reverse transcription-quantitative PCR (RT-qPCR), and then determined anthocyanin content. In total, 94,880 unigenes with a mean length of 635 bp were assembled. The N50 values of the transcripts and unigenes were 2286 bp and 1064 bp, respectively. The functional annotations of the unigenes were analysed against five protein databases. DEGs related to anthocyanin biosynthesis, transportation, and regulation were identified. We also analysed the enrichment pathway, and the differences in mechanisms of anthocyanin induced under low-temperature and high-light conditions are discussed in this paper. This study is the first to examine broad-scale gene expression in Begonia semperflorens. By identifying DEGs regulated by both LT and HL conditions, we found that anthocyanin accumulation was employed as a common strategy by Begonia seedlings in resisting LT and HL stress. By identifying DEGs regulated differently by LT and HL conditions, we found that Begonia seedlings also had some different strategies for resisting LT and HL stresses: anthocyanins were biosynthesized under HL condition, while lignin, proanthocyanidins, and anthocyanins were biosynthesized under LT condition.
Genome-Wide Analysis of Gene and microRNA Expression in Diploid and Autotetraploid Paulownia fortunei (Seem) Hemsl. under Drought Stress by Transcriptome, microRNA, and Degradome Sequencing
Authors: Zhao Zhenli , Niu Suyan , Fan Guoqiang , Deng Minjie , Wang Yuanlong
Published: February 2018

Drought is a common and recurring climatic condition in many parts of the world, and it can have disastrous impacts on plant growth and development. Many genes involved in the drought response of plants have been identified. Transcriptome, microRNA (miRNA), and degradome analyses are rapid ways of identifying drought-responsive genes. The reference genome sequence of Paulownia fortunei (Seem) Hemsl. is now available, which makes it easier to explore gene expression, transcriptional regulation, and post-transcriptional in this species. In this study, four transcriptome, small RNA, and degradome libraries were sequenced by Illumina sequencing, respectively. A total of 258 genes and 11 miRNAs were identified for drought-responsive genes and miRNAs in P. fortunei. Degradome sequencing detected 28 miRNA target genes that were cleaved by members of nine conserved miRNA families and 12 novel miRNAs. The results here will contribute toward enriching our understanding of the response of Paulownia fortunei trees to drought stress and may provide new direction for further experimental studies related the development of molecular markers, the genetic map construction, and other genomic research projects in Paulownia.
Characterization of TTN Novex Splicing Variants across Species and the Role of RBM20 in Novex-Specific Exon Splicing
Authors: Chen Zhilong , Song Jiangping , Chen Liang , Zhu Chaoqun , Cai Hanfang , Sun Mingming , Stern Allysa , Mozdziak Paul , Ge Ying , Means Warrie , Guo Wei
Published: February 2018

Titin (TTN) is a major disease-causing gene in cardiac muscle. Titin (TTN) contains 363 exons in human encoding various sizes of TTN protein due to alternative splicing regulated mainly by RNA binding motif 20 (RBM20). Three isoforms of TTN protein are produced by mutually exclusive exons 45 (Novex 1), 46 (Novex 2), and 48 (Novex 3). Alternatively splicing in Novex isoforms across species and whether Novex isoforms are associated with heart disease remains completely unknown. Cross-species exon comparison with the mVISTA online tool revealed that exon 45 is more highly conserved across all species than exons 46 and 48. Importantly, a conserved region between exons 47 and 48 across species was revealed for the first time. Reverse transcript polymerase chain reaction (RT-PCR) and DNA sequencing confirmed a new exon named as 48′ in Novex 3. In addition, with primer pairs for Novex 1, a new truncated form preserving introns 44 and 45 was discovered. We discovered that Novex 2 is not expressed in the pig, mouse, and rat with Novex 2 primer pairs. Unexpectedly, three truncated forms were identified. One TTN variant with intron 46 retention is mainly expressed in the human and frog heart, another variant with co-expression of exons 45 and 46 exists predominantly in chicken and frog heart, and a third with retention of introns 45 and 46 is mainly expressed in pig, mouse, rat, and chicken. Using Rbm20 knockout rat heart, we revealed that RBM20 is not a splicing regulator of Novex variants. Furthermore, the expression levels of Novex variants in human hearts with cardiomyopathies suggested that Novexes 2 and 3 could be associated with dilated cardiomyopathy (DCM) and/or arrhythmogenic right ventricular cardiomyopathy (ARVC). Taken together, our study reveals that splicing diversity of Novex exons across species and Novex variants might play a role in cardiomyopathy.
A response to the Comment on Tomaskova et al. Mortality in Miners with Coal-Workers’ Pneumoconiosis in the Czech Republic in the Period 1992–2013. Int. J. Environ. Res. Public Health, 2017, 14, 269 by the Author Mei Yong
Authors: Tomášková Hana , Šplíchalová Anna , Šlachtová Hana , Pelclová Daniela , Urban Pavel , Jirák Zdeněk
Published: February 2018

At first, we would like to thank Mei Yong for the comments on our article Mortality in Miners with Coal-Workers’ Pneumoconiosis in the Czech Republic in the Period 1992–2013 [...]
Qualitative Analysis of E-Liquid Emissions as a Function of Flavor Additives Using Two Aerosol Capture Methods
Authors: Eddingsaas Nathan , Pagano Todd , Cummings Cody , Rahman Irfan , Robinson Risa , Hensel Edward
Published: February 2018

This work investigates emissions sampling methods employed for qualitative identification of compounds in e-liquids and their resultant aerosols to assess what capture methods may be sufficient to identify harmful and potentially harmful constituents present. Three popular e-liquid flavors (cinnamon, mango, vanilla) were analyzed using qualitative gas chromatography-mass spectrometry (GC-MS) in the un-puffed state. Each liquid was also machine-puffed under realistic-use flow rate conditions and emissions were captured using two techniques: filter pads and methanol impingers. GC-MS analysis was conducted on the emissions captured using both techniques from all three e-liquids. The e-liquid GC-MS analysis resulted in positive identification of 13 compounds from the cinnamon flavor e-liquid, 31 from mango, and 19 from vanilla, including a number of compounds observed in all e-liquid experiments. Nineteen compounds were observed in emissions which were not present in the un-puffed e-liquid. Qualitative GC-MS analysis of the emissions samples identify compounds observed in all three samples: e-liquid, impinge, and filter pads, and each subset thereof. A limited number of compounds were observed in emissions captured with impingers, but were not observed in emissions captured using filter pads; a larger number of compounds were observed on emissions collected from the filter pads, but not those captured with impingers. It is demonstrated that sampling methods have different sampling efficiencies and some compounds might be missed using only one method. It is recommended to investigate filter pads, impingers, thermal desorption tubes, and solvent extraction resins to establish robust sampling methods for emissions testing of e-cigarette emissions.
Diversity and Antimicrobial Resistance Genotypes in Non-Typhoidal Salmonella Isolates from Poultry Farms in Uganda
Authors: Odoch Terence , Sekse Camilla , L’Abee-Lund Trine , Høgberg Hansen Helge , Kankya Clovice , Wasteson Yngvild
Published: February 2018

Non-typhoidal Salmonella (NTS) are foodborne pathogens of global public health significance. The aim of this study was to subtype a collection of 85 NTS originating from poultry farms in Uganda, and to evaluate a subgroup of phenotypically resistant isolates for common antimicrobial resistance genes and associated integrons. All isolates were subtyped by pulsed-field gel electrophoresis (PFGE). Phenotypically resistant isolates (n = 54) were screened by PCR for the most relevant AMR genes corresponding to their phenotypic resistance pattern, and all 54 isolates were screened by PCR for the presence of integron class 1 and 2 encoding genes. These genes are known to commonly encode resistance to ampicillin, tetracycline, ciprofloxacin, trimethoprim, sulfonamide and chloramphenicol. PFGE revealed 15 pulsotypes representing 11 serotypes from 75 isolates, as 10 were non-typable. Thirty one (57.4%) of the 54 resistant isolates carried at least one of the seven genes (blaTEM-1, cmlA, tetA, qnrS, sul1, dhfrI, dhfrVII) identified by PCR and six (11%) carried class 1 integrons. This study has shown that a diversity of NTS-clones are present in Ugandan poultry farm settings, while at the same time similar NTS-clones occur in different farms and areas. The presence of resistance genes to important antimicrobials used in human and veterinary medicine has been demonstrated, hence the need to strengthen strategies to combat antimicrobial resistance at all levels.
Are Kazakhstani Women Satisfied with Antenatal Care? Implementing the WHO Tool to Assess the Quality of Antenatal Services
Authors: Dauletyarova Marzhan , Semenova Yuliya , Kaylubaeva Galiya , Manabaeva Gulshat , Toktabayeva Bakytkul , Zhelpakova Maryash , Yurkovskaya Oxana , Tlemissov Aidos , Antonova Galina , Grjibovski Andrej
Published: February 2018

Women’s satisfaction is a part of the quality assurance process with potential to improve antenatal health services. The objective of this study was to assess the prevalence of women’s satisfaction with antenatal care in an urban Kazakhstani setting and investigate associated factors. A total of 1496 women who delivered in all maternity clinics from 6 February through 11 July 2013 in Semey, East Kazakhstan, filled out a standardized pretested questionnaire on satisfaction with antenatal care. Independent associations between dissatisfaction and its correlates were studied by logistic regression. Ninety percent of the women were satisfied with the antenatal care. Women who were dissatisfied had lower education. These women would have preferred more checkups, shorter intervals between checkups, more time with care providers, and shorter waiting times. The overall dissatisfaction was associated with long waiting times and insufficient information on general health in pregnancy, results of laboratory tests, treatment during pregnancy, and breastfeeding. Although most of the women in the study setting were satisfied with the new antenatal care model, we identified the main sources of dissatisfaction that should be addressed. Given that Semey is a typical Kazakhstani city, the results can be generalized to other Kazakhstani urban settings.
A Comparative Analysis of Climate-Risk and Extreme Event-Related Impacts on Well-Being and Health: Policy Implications
Authors: Leal Filho Walter , Al-Amin Abul , Nagy Gustavo , Azeiteiro Ulisses , Wiesböck Laura , Ayal Desalegn , Morgan Edward , Mugabe Paschal , Aparicio-Effen Marilyn , Fudjumdjum Hubert , Chiappetta Jabbour Charbel
Published: February 2018

There are various climate risks that are caused or influenced by climate change. They are known to have a wide range of physical, economic, environmental and social impacts. Apart from damages to the physical environment, many climate risks (climate variability, extreme events and climate-related hazards) are associated with a variety of impacts on human well-being, health, and life-supporting systems. These vary from boosting the proliferation of vectors of diseases (e.g., mosquitos), to mental problems triggered by damage to properties and infrastructure. There is a great variety of literature about the strong links between climate change and health, while there is relatively less literature that specifically examines the health impacts of climate risks and extreme events. This paper is an attempt to address this knowledge gap, by compiling eight examples from a set of industrialised and developing countries, where such interactions are described. The policy implications of these phenomena and the lessons learned from the examples provided are summarised. Some suggestions as to how to avert the potential and real health impacts of climate risks are made, hence assisting efforts to adapt to a problem whose impacts affect millions of people around the world. All the examples studied show some degree of vulnerability to climate risks regardless of their socioeconomic status and need to increase resilience against extreme events.
Work Stress Interventions in Hospital Care: Effectiveness of the DISCovery Method
Authors: Niks Irene , De Jonge Jan , Gevers Josette , Houtman Irene
Published: February 2018

Effective interventions to prevent work stress and to improve health, well-being, and performance of employees are of the utmost importance. This quasi-experimental intervention study presents a specific method for diagnosis of psychosocial risk factors at work and subsequent development and implementation of tailored work stress interventions, the so-called DISCovery method. This method aims at improving employee health, well-being, and performance by optimizing the balance between job demands, job resources, and recovery from work. The aim of the study is to quantitatively assess the effectiveness of the DISCovery method in hospital care. Specifically, we used a three-wave longitudinal, quasi-experimental multiple-case study approach with intervention and comparison groups in health care work. Positive changes were found for members of the intervention groups, relative to members of the corresponding comparison groups, with respect to targeted work-related characteristics and targeted health, well-being, and performance outcomes. Overall, results lend support for the effectiveness of the DISCovery method in hospital care.
Examining the Influence of a New Light Rail Line on the Health of a Demographically Diverse and Understudied Population within the Washington, D.C. Metropolitan Area: A Protocol for a Natural Experiment Study
Authors: Roberts Jennifer D. , Hu Ming , Saksvig Brit Irene , Brachman Micah L. , Durand Casey P.
Published: February 2018

Approximately two-thirds of adults and youth in Prince George’s County, Maryland, a suburb of Washington, D.C. are overweight or obese and less than half are achieving daily physical activity recommendations. Active transportation (AT), such as walking, biking or using public transportation (PT), is a strategic pathway to improving physical activity levels and thus reducing excess weight. Utilizing an expansion of the Washington, D.C. area transportation system with a new light rail line, the Purple Line Outcomes on Transportation (PLOT) Study will exam pre- and post-Purple Line PT use, AT behaviors and attitudes and physical activity among Prince George’s County adults and youth. The PLOT Study will take advantage of this natural experiment in an area enduring significant racial/ethnic and gender-based overweight or obesity and physical inactivity disparities. While similar natural experiments on AT have been conducted in other U.S. cities, those studies lacked diverse and representative samples. To effectively evaluate these physical activity outcomes among this population, efforts will be used to recruit African American and Latino populations, the first and second most common racial/ethnic groups in Prince George’s County. Finally, the PLOT Study will also examine how contextual effects (e.g., neighborhood built environment) impact PT, AT and physical activity.
The Crosstalk between Nrf2 and Inflammasomes
Authors: Hennig Paulina , Garstkiewicz Martha , Grossi Serena , Di Filippo Michela , French Lars , Beer Hans-Dietmar
Published: February 2018

The Nrf2 (nuclear factor E2-related factor or nuclear factor (erythroid-derived 2)-like 2) transcription factor is a key player in cytoprotection and activated in stress conditions caused by reactive oxygen species (ROS) or electrophiles. Inflammasomes represent central regulators of inflammation. Upon detection of various stress factors, assembly of the inflamasome protein complex results in activation and secretion of proinflammatory cytokines. In addition, inflammasome activation causes pyroptosis, a lytic form of cell death, which supports inflammation. There is growing evidence of a crosstalk between the Nrf2 and inflammasome pathways at different levels. For example, Nrf2 activating compounds inhibit inflammasomes and consequently inflammation. This review summarizes what is known about the complex and predominantly antagonistic relationship of both stress-activated pathways.
Mitochondrial Dynamics in Basal and Stressful Conditions
Authors: Zemirli Naima , Morel Etienne , Molino Diana
Published: February 2018

The historical role of mitochondria resides in converting the energy released during the oxidation of macromolecules (carbohydrates, lipids and proteins) into adenosine tri-phosphate, a major form of chemically stored energy which sustains cell growth and homeostasis. Beyond this role in bioenergetics regulation, mitochondria play a role in several other cellular processes including lipid metabolism, cellular calcium homeostasis, autophagy and immune responses. Furthermore, mitochondria are highly dynamic organelles: as all other cellular endomembranes, they are continuously moving along cytoskeleton, and, most importantly, they constantly interact one with each other by membrane tethering, fusion and fission. This review aims to highlight the tight correlation between the morphodynamics of mitochondria and their biological function(s), in physiological as well as stress conditions, in particular nutrient deprivation, pathogen attack and some human diseases. Finally, we emphasize some crosstalk between the fusion/fission machinery and the autophagy pathway to ending on some speculative hypothesis to inspire future research in the field.
Sjögren-Like Lacrimal Keratoconjunctivitis in Germ-Free Mice
Authors: Wang Changjun , Zaheer Mahira , Bian Fang , Quach Darin , Swennes Alton , Britton Robert , Pflugfelder Stephen , De Paiva Cintia
Published: February 2018

Commensal bacteria play an important role in the formation of the immune system but their role in the maintenance of immune homeostasis at the ocular surface and lacrimal gland remains poorly understood. This study investigated the eye and lacrimal gland phenotype in germ-free and conventional C57BL/6J mice. Our results showed that germ-free mice had significantly greater corneal barrier disruption, greater goblet cell loss, and greater total inflammatory cell and CD4+ T cell infiltration within the lacrimal gland compared to the conventionally housed group. A greater frequency of CD4+IFN-γ+ cells was observed in germ-free lacrimal glands. Females exhibited a more severe phenotype compared to males. Adoptive transfer of CD4+ T cells isolated from female germ-free mice into RAG1KO mice transferred Sjögren-like lacrimal keratoconjunctivitis. Fecal microbiota transplant from conventional mice reverted dry eye phenotype in germ-free mice and decreased CD4+IFN-γ+ cells to levels similar to conventional C57BL/6J mice. These findings indicate that germ-free mice have a spontaneous lacrimal keratoconjunctivitis similar to that observed in Sjögren syndrome patients and demonstrate that commensal bacteria function in maintaining immune homeostasis on the ocular surface. Thus, manipulation of intestinal commensal bacteria has the potential to become a novel therapeutic approach to treat Sjögren Syndrome.
Clausal Coordination in Gã: The Case of nì
Authors: Nartey Mark
Published: February 2018

This paper analyses the communicative functions of the Gã (Niger–Congo, Kwa branch) clausal connective nì, roughly corresponding to English ‘and’, drawing on Wilson and Sperber’s (1995; 2004) relevance theory. The study demonstrates that nì is a conjunction that achieves optimal relevance when the nì-utterance has cognitive effects that transcend those of the individual conjuncts of nì. Importantly, the use of nì leads the way in providing clues that aid the interlocutor to “work out” the nature of the inferential relation between the conjuncts. These inferential relations, including addition, temporality, causality, contrast and parallelism, ultimately enable the interlocutor to arrive at the desired and/or intended interpretation of the utterance as a whole. This paper is, thus, an attempt to account for the meaning of nì as well as the pragmatic processes that inform the specific inferential relations between the nì-conjuncts. It also illumines the ways in which pragmatic enrichment and context-dependent inference can strengthen the logical form of clausal coordination in Gã.
HDPE/Chitosan Blends Modified with Organobentonite Synthesized with Quaternary Ammonium Salt Impregnated Chitosan
Authors: De Araújo Maria , Barbosa Rossemberg , Fook Marcus , Canedo Eduardo , Silva Suédina , Medeiros Eliton , Leite Itamara
Published: February 2018

In this study, blends based on a high density polyethylene (HDPE) and chitosan (CS) were successfully prepared by melt processing, in a laboratory internal mixer. The CS biopolymer content effect (up to maximum of 40%), and, the addition of bentonite clay modified with quaternary ammonium salt (CTAB) impregnated chitosan as a compatibilizing agent, on the properties of the blends was analyzed by Fourier transform-infrared spectroscopy (FT-IR), wide angle X-ray diffraction (WAXD), differential scanning calorimetry (DSC), thermogravimetric analyses (TG), tensile strength, and scanning electron microscopy (SEM). The use of clay modified with CTAB impregnated chitosan, employing a method developed here, improved the compatibility of HDPE with chitosan, and therefore the thermal and some of the mechanical properties were enhanced, making HDPE/chitosan blends suitable candidates for food packaging. It was possible to obtain products of synthetic polymer, HDPE, with natural polymer, chitosan, using a method very used industrially, with acceptable and more friendly properties to the environment, when compared to conventional synthetic polymers. In addition, due to the possibility of impregnated chitosan with quaternary ammonium salt exhibit higher antibacterial activity than neat chitosan, the HDPE/chitosan/organobentonite blends may be potentially applied in food containers to favor the preservation of food for a longer time in comparison to conventional materials.
Protective Performance of Polyaniline-Sulfosalicylic Acid/Epoxy Coating for 5083 Aluminum
Authors: Liu Suyun , Liu Li , Meng Fandi , Li Ying , Wang Fuhui
Published: February 2018

Epoxy coatings incorporating different content of sulfosalicylic acid doped polyaniline (PANI-SSA) have been investigated for corrosion protection of 5083 aluminum alloy in 3.5% NaCl solution. The performance of the coatings is studied using a combination of electrochemical impedance spectroscopy (EIS), open circuit potential (OCP), gravimetric tests, adhesion tests, scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). The results demonstrate that the content of PANI-SSA not only affects the coating compactness and the transportation of aggressive medium, but also has a significant influence on the-based aluminum. The coating with 2 wt. % PANI-SSA exhibits the best corrosion inhibition due to its good protective properties and the formation of a complete PANI-SSA induced oxide layer.
Thermoauxetic Behavior of Composite Structures
Authors: Jopek Hubert , Stręk Tomasz
Published: February 2018

This paper presents a study of new two-dimensional composite structures with respect to their thermomechanical properties. The investigated structures are based on very well-known auxetic geometries—i.e., the anti-tetrachiral and re-entrant honeycomb—modified by additional linking elements, material which is highly sensitive to changes of temperature. The study shows that temperature can be used as a control parameter to tune the value of the effective Poisson’s ratio, which allows, in turn, changing its value from positive to negative, according to the temperature applied. The study shows that such thermoauxetic behavior applies both to composites with voids and those completely filled with material.
Tubular Micro/Nanomotors: Propulsion Mechanisms, Fabrication Techniques and Applications
Authors: Zha Fengjun , Wang Tingwei , Luo Ming , Guan Jianguo
Published: February 2018

Micro/nanomotors are self-propelled machines that can convert various energy sources into autonomous movement. With the great advances of nanotechnology, Micro/Nanomotors of various geometries have been designed and fabricated over the past few decades. Among them, the tubular Micro/Nanomotors have a unique morphology of hollow structures, which enable them to possess a strong driving force and easy surface functionalization. They are promising for environmental and biomedical applications, ranging from water remediation, sensing to active drug delivery and precise surgery. This article gives a comprehensive and clear review of tubular Micro/Nanomotors, including propulsion mechanisms, fabrication techniques and applications. In the end, we also put forward some realistic problems and speculate about corresponding methods to improve existing tubular Micro/Nanomotors.
Perceptual Surgical Knife with Wavelet Denoising
Authors: Li Tao , Sunami Yuta , Zhang Sheng
Published: February 2018

Robotic surgery is a new technology in medical applications and has been undergoing rapid development. The surgical knife, essential for robotic surgery, has the ability to determine the success of an operation. In this paper, on the basis of the principle of field-effect transistors (FETs), a perceptual surgical knife is proposed to detect the electrons or electric field of the human body with distinguishable signals. In addition, it is difficult to discriminate between the motions of surgical knives from the perceptual signals that are disturbed by high-frequency Gaussian white noise. Therefore, the wavelet denoising approach is chosen to reduce the high-frequency noise. The proposed perceptual surgical knife with the wavelet denoising method has the characteristics of high sensitivity, low cost, and good repeatability.
Mining and Development of Novel SSR Markers Using Next Generation Sequencing (NGS) Data in Plants
Authors: Taheri Sima , Lee Abdullah Thohirah , Yusop Mohd , Hanafi Mohamed , Sahebi Mahbod , Azizi Parisa , Shamshiri Redmond
Published: February 2018

Microsatellites, or simple sequence repeats (SSRs), are one of the most informative and multi-purpose genetic markers exploited in plant functional genomics. However, the discovery of SSRs and development using traditional methods are laborious, time-consuming, and costly. Recently, the availability of high-throughput sequencing technologies has enabled researchers to identify a substantial number of microsatellites at less cost and effort than traditional approaches. Illumina is a noteworthy transcriptome sequencing technology that is currently used in SSR marker development. Although 454 pyrosequencing datasets can be used for SSR development, this type of sequencing is no longer supported. This review aims to present an overview of the next generation sequencing, with a focus on the efficient use of de novo transcriptome sequencing (RNA-Seq) and related tools for mining and development of microsatellites in plants.
Synthesis and Evaluation of the Tumor Cell Growth Inhibitory Potential of New Putative HSP90 Inhibitors
Authors: Bizarro Ana , Sousa Diana , Lima Raquel , Musso Loana , Cincinelli Raffaella , Zuco Vantina , De Cesare Michelandrea , Dallavalle Sabrina , Vasconcelos M.
Published: February 2018

Background: Heat shock protein 90 (HSP90) is a well-known target for cancer therapy. In a previous work, some of us have reported a series of 3-aryl-naphtho[2,3-d]isoxazole-4,9-diones as inhibitors of HSP90. Methods: In the present work, various compounds with new chromenopyridinone and thiochromenopyridinone scaffolds were synthesized as potential HSP90 inhibitors. Their binding affinity to HSP90 was studied in vitro. Selected compounds (5 and 8) were further studied in various tumor cell lines regarding their potential to cause cell growth inhibition, alter the cell cycle profile, inhibit proliferation, and induce apoptosis. Their effect on HSP90 client protein levels was also confirmed in two cell lines. Finally, the antitumor activity of compound 8 was studied in A431 squamous cell carcinoma xenografts in nude mice. Results: Our results indicated that treatment with compounds 5 and 8 decreased the proliferation of tumor cell lines and compound 8 induced apoptosis. In addition, these two compounds were able to downregulate selected proteins known as “clients” of HSP90. Finally, treatment of xenografted mice with compound 5 resulted in a considerable dose-dependent inhibition of tumor growth. Conclusions: Our results show that two new compounds with a chromenopyridinone and thiochromenopyridinone scaffold are promising putative HSP90 inhibitors causing tumor cell growth inhibition.
Phytochemical Analysis, Antioxidant and Antimicrobial Activities of Helichrysum arenarium (L.) Moench. and Antennaria dioica (L.) Gaertn. Flowers
Authors: Babotă Mihai , Mocan Andrei , Vlase Laurian , Crișan Ovidiu , Ielciu Irina , Gheldiu Ana-Maria , Vodnar Dan , Crișan Gianina , Păltinean Ramona
Published: February 2018

Antennaria dioica (L.) Gaertn. and Helichrysum arenarium (L.) Moench. are two species of the Asteraceae family, known in Romanian traditional medicine for their diuretic, choleretic, and anti-inflammatory properties. The aim of the present study was to evaluate the phenolic and sterolic composition of flowers from the two species and to assess their antioxidant, antibacterial and antifungal properties. LC-MS analyses were performed on methanolic, ethanolic and 70% v/v ethanolic extracts, before and after acid hydrolysis, and revealed high amounts of polyphenols. Chlorogenic acid was found as the main compound for the flowers of A. dioica (502.70 ± 25.11 mg/100 g d.w.), while quercitrin was dominant in H. arenarium (424.28 ± 21.21 mg/100 g d.w.) in 70% v/v ethanolic extracts before hydrolysis. Antioxidant capacity assays showed an important antioxidant potential, which can be correlated with the determined polyphenolic compounds, showing the 70% v/v ethanolic extracts of the two species as being the most effective antioxidant samples for the DPPH assay. Antibacterial and antifungal assays confirm a modest biological potential for the same extract of both species. Results obtained in the present study bring important data and offer scientific evidence on the chemical composition and on the biological activities of the flowers belonging to the two species.