bluuebunny/crossref_metadata_2025 huggingface dataset:
Vibration Emissions Reduce Boar Sperm Quality via Disrupting Its Metabolism
Authors: Wang Shanpeng , Zeng Xuejun , Liu Shenao , Hoque S. A. Masudul , Min Lingjiang , Ding Nengshui , Zhu Zhendong
Published: May 2024

Artificial insemination (AI) with liquid-preserved semen has recently become common in pig breeding. The semen doses are produced in a centralized manner at the boar stud and then subsequently distributed and transported to sow farms. However, vibration emissions during transportation by logistic vehicles may adversely affect the quality of boar sperm. Therefore, this study aimed to explore the impact of vibration-induced emissions on sperm quality and function under simulated transportation conditions. Each time, ejaculates from all 15 boars were collected and then pooled together to minimize individual variations, and the sample was split using an extender for dilution. Different rotational speeds (0 rpm, 80 rpm, 140 rpm, 200 rpm) were utilized to simulate varying intensities of vibration exposure using an orbital shaker, considering different transportation times (0 h, 3 h, and 6 h). Subsequently, evaluations were conducted regarding sperm motility, plasma membrane integrity, acrosome integrity, mitochondrial function, adenosine triphosphate (ATP) levels, mitochondrial reactive oxygen species (ROS) levels, pH, glycolytic pathway enzyme activities, and capacitation following exposure to vibration emissions. Both vibration time and intensity impact sperm motility, plasma membrane integrity, and acrosomal integrity. Vibration exposure significantly reduced sperm ATP levels, mitochondrial membrane potential, and the levels of mitochondria-encoded proteins (MT-ND1, MT-ND6) (p < 0.05). After vibration emission treatment, the pH value and mitochondrial ROS levels significantly increased (p < 0.05). Inhibition of sperm glycolysis was observed, with reduced activities of hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase (LDH), along with decreased lactate levels (p < 0.05). Additionally, sperm tyrosine phosphorylation levels were significantly reduced by vibration emissions compared to the control group (p < 0.05). After the vibration emission treatment, the number of sperm bound to each square millimeter of oviduct explants decreased significantly compared to the control group (p < 0.05). Similarly, compared to the control group, using semen subjected to vibration stress for AI results in significantly reduced pregnancy rates, total born litter size, live-born litter size, and healthy born litter size (p < 0.05).
Microfluidic System-Based Quantitative Analysis of Platelet Function through Speckle Size Measurement
Authors: Han Jong Hyeok , Yoon Inkwon , Jeon Hee-Jae
Published: May 2024

Platelets play essential roles in the formation of blood clots by clumping with coagulation factors at the site of vascular injury to stop bleeding; therefore, a reduction in the platelet number or disorder in their function causes bleeding risk. In our research, we developed a method to assess platelet aggregation using an optical approach within a microfluidic chip’s channel by evaluating the size of laser speckles. These speckles, associated with slowed blood flow in the microfluidic channel, had a baseline size of 28.54 ± 0.72 µm in whole blood. Removing platelets from the sample led to a notable decrease in speckle size to 27.04 ± 1.23 µm. Moreover, the addition of an ADP-containing agonist, which activates platelets, resulted in an increased speckle size of 32.89 ± 1.69 µm. This finding may provide a simple optical method via microfluidics that could be utilized to assess platelet functionality in diagnosing bleeding disorders and potentially in monitoring therapies that target platelets.
Crystal Violet Selectively Detects Aβ Oligomers but Not Fibrils In Vitro and in Alzheimer’s Disease Brain Tissue
Authors: Karunarathne Kanchana , Kee Teresa R. , Jeon Hanna , Cazzaro Sara , Gamage Yasith I. , Pan Jianjun , Woo Jung-A. A. , Kang David E. , Muschol Martin
Published: May 2024

Deposition of extracellular Amyloid Beta (Aβ) and intracellular tau fibrils in post-mortem brains remains the only way to conclusively confirm cases of Alzheimer’s Disease (AD). Substantial evidence, though, implicates small globular oligomers instead of fibrils as relevant biomarkers of, and critical contributors to, the clinical symptoms of AD. Efforts to verify and utilize amyloid oligomers as AD biomarkers in vivo have been limited by the near-exclusive dependence on conformation-selective antibodies for oligomer detection. While antibodies have yielded critical evidence for the role of both Aβ and tau oligomers in AD, they are not suitable for imaging amyloid oligomers in vivo. Therefore, it would be desirable to identify a set of oligomer-selective small molecules for subsequent development into Positron Emission Tomography (PET) probes. Using a kinetics-based screening assay, we confirm that the triarylmethane dye Crystal Violet (CV) is oligomer-selective for Aβ42 oligomers (AβOs) grown under near-physiological solution conditions in vitro. In postmortem brains of an AD mouse model and human AD patients, we demonstrate that A11 antibody-positive oligomers but not Thioflavin S (ThioS)-positive fibrils colocalize with CV staining, confirming in vitro results. Therefore, our kinetic screen represents a robust approach for identifying new classes of small molecules as candidates for oligomer-selective dyes (OSDs). Such OSDs, in turn, provide promising starting points for the development of PET probes for pre-mortem imaging of oligomer deposits in humans.
Enhancement of Biogas (Methane) Production from Cow Dung Using a Microbial Electrochemical Cell and Molecular Characterization of Isolated Methanogenic Bacteria
Authors: Bhatt Puja , Poudyal Pranita , Dhungana Pradip , Prajapati Bikram , Bajracharya Suman , Yadav Amar Prasad , Bhattarai Tribikram , Sreerama Lakshmaiah , Joshi Jarina
Published: May 2024

Biogas has long been used as a household cooking fuel in many tropical counties, and it has the potential to be a significant energy source beyond household cooking fuel. In this study, we describe the use of low electrical energy input in an anaerobic digestion process using a microbial electrochemical cell (MEC) to promote methane content in biogas at 18, 28, and 37 °C. Although the maximum amount of biogas production was at 37 °C (25 cm3), biogas could be effectively produced at lower temperatures, i.e., 18 (13 cm3) and 28 °C (19 cm3), with an external 2 V power input. The biogas production of 13 cm3 obtained at 18 °C was ~65-fold higher than the biogas produced without an external power supply (0.2 cm3). This was further enhanced by 23% using carbon-nanotubes-treated (CNT) graphite electrodes. This suggests that the MEC can be operated at as low as 18 °C and still produce significant amounts of biogas. The share of CH4 in biogas produced in the controls was 30%, whereas the biogas produced in an MEC had 80% CH4. The MEC effectively reduced COD to 42%, whereas it consumed 98% of reducing sugars. Accordingly, it is a suitable method for waste/manure treatment. Molecular characterization using 16s rRNA sequencing confirmed the presence of methanogenic bacteria, viz., Serratia liquefaciens and Zoballella taiwanensis, in the inoculum used for the fermentation. Consistent with recent studies, we believe that electromethanogenesis will play a significant role in the production of value-added products and improve the management of waste by converting it to energy.
Number of Premature Ventricular Complexes Predicts Long-Term Outcomes in Patients with Persistent Atrial Fibrillation
Authors: Yen Kun-Chi , Chan Yi-Hsin , Wang Chun-Li
Published: May 2024

Background: Premature ventricular complexes (PVCs) are common electrocardiographic abnormalities and may be a prognosticator in predicting mortality in patients with structurally normal hearts or chronic heart diseases. Whether PVC burden was associated with mortality in patients with chronic atrial fibrillation (AF) remained unknown. We investigated the prognostic value of PVC burden in patients with persistent AF. Methods: A retrospective analysis of 24 h Holter recordings of 1767 patients with persistent AF was conducted. Clinical characteristics, 24 h average heart rate (HR), and PVC measures, including 24 h PVC burden and the presence of consecutive PVCs (including any PVC couplet, triplet, or non-sustained ventricular tachycardia) were examined for the prediction of all-cause and cardiovascular mortality using the Cox proportional hazards model. Results: After a median follow-up time of 30 months, 286 (16%) patients died and 1481 (84%) patients survived. Multivariate analysis revealed that age, heart failure, stroke, angiotensin-converting enzyme inhibitor/angiotensin receptor blocker, beta-blocker, digoxin, oral anticoagulant use, and estimated glomerular filtration rate were significant baseline predictors of all-cause mortality and cardiovascular mortality. Twenty-four-hour PVC burden and the presence of consecutive PVCs were significantly associated with all-cause and cardiovascular mortality after adjusting for significant clinical factors. When compared to the first quartile of PVC burden (<0.003%/day), the highest quartile (>0.3%/day) was significantly associated with an increased risk of all-cause mortality (hazard ratio, 2.46; 95% CI, 1.77–3.42) and cardiovascular mortality (hazard ratio: 2.67; 95% CI, 1.76–4.06). Conclusions: Twenty-four-hour PVC burden is independently associated with all-cause and cardiovascular mortality in patients with persistent AF.
Polydopamine Coating of Graphitic Carbon Nitride, g-C3N4, Improves Biomedical Application
Authors: Sahiner Mehtap , Demirci Sahin , Sahiner Nurettin
Published: May 2024

Graphitic carbon nitride (g-C3N4) is an intriguing nanomaterial that exhibits photoconductive fluorescence properties under UV–visible light. Dopamine (DA) coating of g-C3N4 prepared from melamine was accomplished via self-polymerization of DA as polydopamine (PDA). The g-C3N4 was coated with PDA 1, 3, and 5 times repeatedly as (PDA@g-C3N4) in tris buffer at pH 8.5. As the number of PDA coatings was increased on g-C3N4, the peak intensity at 1512 cm−1 for N–H bending increased. In addition, the increased weight loss values of PDA@g-C3N4 structures at 600 °C from TGA thermograms confirmed that the coating was accomplished. The band gap of g-C3N4, 2.72 eV, was reduced to 0.87 eV after five coatings with PDA. A pristine g-C3N4 was found to have an isoelectric point (IEP) of 4.0, whereas the isoelectric points of 1PDA@g-C3N4 and 3PDA@g-C3N4 are close to each other at 3.94 and 3.91, respectively. On the other hand, the IEP of 5PDA@g-C3N4 was determined at pH 5.75 assuming complete coating with g-C3N4. The biocompatibility of g-C3N4 and PDA@g-C3N4 against L929 fibroblast cell lines revealed that all PDA@g-C3N4 coatings were found to be biocompatible up to a 1000 mg/mL concentration, establishing that PDA coatings did not adversely affect the biocompatibility of the composite materials. In addition, PDA@g-C3N4 was screened for antioxidant potential via total phenol content (TPC) and total flavonoid content assays and it was found that PDA@g-C3N4 has recognizable TPC values and increased linearly with an increased number of PDA coatings. Furthermore, blood compatibility of pristine g-C3N4 is enhanced considerably upon PDA coating, affirmed by hemolysis and the blood clotting index%. Additionally, α-glucosidase inhibitory properties of PDA@g-C3N4 structures revealed that 67.6 + 9.8% of this enzyme was evenly inhibited by 3PDA@g-C3N4 structure.
Mass Spectrometry as Alternative Method to Identify and Monitor Non-Secretory Progressive Disease in Patients with Multiple Myeloma
Authors: Agulló Cristina , Puig Noemí , Contreras Teresa , Castro Sergio , Puertas Borja , González-Calle Verónica , Rey-Búa Beatriz , Mateos María Victoria
Published: May 2024

Introduction: After receiving different lines of treatment, multiple myeloma patients tend to present with less secretory and more frequent extramedullary disease. These features make treatment monitoring and follow-up very complex since they have to be based on the use of imaging methods and/or bone marrow aspirations or biopsies. Objective: To present the case of a patient with myeloma progressing with non-secretory bone disease and to discuss the potential impact of mass spectrometry as a new highly sensitive method able to identify the monoclonal protein (MP) in the serum of these types of patients. Materials and Methods: Informed consent was signed by the patient prior to receiving each line of treatment. The clinical information and images were obtained from anonymized electronic files. The mass spectrometry was performed with the Immunoglobulin Isotypes (GAM) assay for the mass spectrometry EXENT® Analyser Technology from Binding Site, part of Thermofisher. Results: A 73-year-old male with IgG kappa multiple myeloma progressing with a new lytic lesion after receiving 14 cycles of Talquetamab as a third line of therapy who, due to the non-secretory nature of the disease at this point, could not be enrolled in a clinical trial, thus limiting his therapeutic options. The mass spectrometry was able to identify and quantify the presence of the patient’s MP when the serum protein electrophoresis and immunofixation were still negative and therefore could have been used to confirm the progression, to permit the inclusion of the patient in a clinical trial and to further monitor the disease response. Conclusions: The higher sensitivity of the mass spectrometry methods to detect the MP in patients with myeloma and other monoclonal gammopathies translates into better identification of the disease progression, permits the inclusion of more patients in clinical trials and facilitates treatment monitoring.
Design and Validation of the Trailing Edge of a Variable Camber Wing Based on a Two-Dimensional Airfoil
Authors: Zhou Jin , Sun Xiasheng , Sun Qixing , Xue Jingfeng , Song Kunling , Li Yao , Dong Lijun
Published: May 2024

Variable camber wing technology stands out as the most promising morphing technology currently available in green aviation. Despite the ongoing advancements in smart materials and compliant structures, they still fall short in terms of driving force, power, and speed, rendering mechanical structures based on kinematics the preferred choice for large long-range civilian aircraft. In line with this principle, this paper introduces a linkage-based variable camber trailing edge design approach. Covering coordinated design, internal skeleton design, flexible skin design, and drive structure design, the method leverages a two-dimensional supercritical airfoil to craft a seamless, continuous two-dimensional wing full-size variable camber trailing edge structure, boasting a 2.7 m span and 4.3 m chord. Given the significant changes in aerodynamic load direction, ground tests under cruise load utilize a tracking-loading system based on tape and lever. Results indicate that the designed single-degree-of-freedom Watt I mechanism and Stephenson III drive mechanism adeptly accommodate the slender trailing edge of the supercritical airfoil. Under a maximum cruise vertical aerodynamic load of 17,072 N, the structure meets strength requirements when deflected to 5°. The research in this paper can provide some insights into the engineering design of variable camber wings.
Optimization of Microfluidics for Point-of-Care Blood Sensing
Authors: Tavakolidakhrabadi Amirmahdi , Stark Matt , Bacher Ulrike , Legros Myriam , Bessire Cedric
Published: May 2024

Blood tests are widely used in modern medicine to diagnose certain illnesses and evaluate the overall health of a patient. To enable testing in resource-limited areas, there has been increasing interest in point-of-care (PoC) testing devices. To process blood samples, liquid mixing with active pumps is usually required, making PoC blood testing expensive and bulky. We explored the possibility of processing approximately 2 μL of whole blood for image flow cytometry using capillary structures that allowed test times of a few minutes without active pumps. Capillary pump structures with five different pillar shapes were simulated using Ansys Fluent to determine which resulted in the fastest whole blood uptake. The simulation results showed a strong influence of the capillary pump pillar shape on the chip filling time. Long and thin structures with a high aspect ratio exhibited faster filling times. Microfluidic chips using the simulated pump design with the most efficient blood uptake were fabricated with polydimethylsiloxane (PDMS) and polyethylene oxide (PEO). The chip filling times were tested with 2 μL of both water and whole blood, resulting in uptake times of 24 s for water and 111 s for blood. The simulated blood plasma results deviated from the experimental filling times by about 35% without accounting for any cell-induced effects. By comparing the flow speed induced by different pump pillar geometries, this study offers insights for the design and optimization of passive microfluidic devices for inhomogenous liquids such as whole blood in sensing applications.
Research on the Design Strategies of Public Outdoor Spaces in Government Centers from a Health Perspective
Authors: Wang Yilin , Peng Wenjun , Huang Yanyan , Yang Tiancheng , Du Xinyu , Wang Xinyan , Zhang Zhengming , Zhu Liang
Published: May 2024

This study delves into an investigation of urban public outdoor spaces (POSs) from a health-oriented perspective, recognizing varied health needs encompassing physical, psychological, social, and environmental aspects. In this study, POSs of two typical government service centers (GSCs) were analyzed based on their structure, user demographics, and user satisfaction, revealing several problems with the current GSC POS designs. To address these problems, principles for GSC POS design were proposed for natural, playing, and social spaces and applied to redesign the Wuchang GSC. Firstly, through on-site surveys, questionnaire surveys, and data analysis, the existing problems in promoting residents’ health in the GSC POSs were revealed, such as the insufficient greening of natural spaces, lack of interest in playing spaces, and unreasonable design of the scale of social spaces. Based on the above analysis, a health design optimization principle based on Maslow’s theory is proposed. Firstly, improvement solutions were proposed and implemented for green spaces, such as using more diversified natural elements, zone differentiation, and landscape improvements to promote the health of users. Secondly, the leisure and sports needs of different age groups can be met simultaneously by diversifying the layout and functional settings of playing spaces. Finally, public spaces suitable for social interaction were redesigned to promote the psychological health of citizens in social activities by optimizing the scale of communication spaces. The proposed design optimization strategies for GSCs not only provide theoretical support for the healthy design of POSs but also provide useful references for the healthy development of urban public spaces.
A Review of Static and Dynamic p-y Curve Models for Pile Foundations
Authors: Wu Jiujiang , Pu Longjun , Zhai Changming
Published: May 2024

In addition to supporting vertical loads from superstructures, piles are frequently subjected to horizontal soil pressures, long-term wind, wave, and current forces, as well as seismic loads. Presently, the p-y curve method is widely employed for calculating the horizontal forces acting on piles due to its ability to replicate the nonlinear interaction between piles and soil. This paper provides a thorough review and analysis of the current research on p-y curve models for piles, examining literature across various conditions such as horizontal static loads, cyclic loads, and seismic loads. Special emphasis is placed on the development, classification, and analysis of the key factors influencing major p-y curve models. It also discusses future research directions and prospects, considering emerging trends and prevailing challenges in the field. For instance, future studies should investigate p-y curves for piles under various combined loads, considering the influence of construction methods and the installation effect. Additionally, the development of a comprehensive p-y curve database and the application of existing research to new foundation systems are essential for advancing pile technology and fostering innovative designs.
Practical Application of Deep Learning in Diagnostic Neuropathology—Reimagining a Histological Asset in the Era of Precision Medicine
Authors: Rich Katherine , Tosefsky Kira , Martin Karina C. , Bashashati Ali , Yip Stephen
Published: May 2024

In the past few decades, neuropathology has experienced several paradigm shifts with the introduction of new technologies. Deep learning, a rapidly progressing subfield of machine learning, seems to be the next innovation to alter the diagnostic workflow. In this review, we will explore the recent changes in the field of neuropathology and how this has led to an increased focus on molecular features in diagnosis and prognosis. Then, we will examine the work carried out to train deep learning models for various diagnostic tasks in neuropathology, as well as the machine learning frameworks they used. Focus will be given to both the challenges and successes highlighted therein, as well as what these trends may tell us about future roadblocks in the widespread adoption of this new technology. Finally, we will touch on recent trends in deep learning, as applied to digital pathology more generally, and what this may tell us about the future of deep learning applications in neuropathology.
Complications and Risk Factors in Patients with Soft Tissue Sarcoma of the Extremities Treated with Radiotherapy
Authors: Lebas Arthur , Le Fevre Clara , Waissi Waisse , Chambrelant Isabelle , Brinkert David , Noel Georges
Published: May 2024

Introduction: Soft tissue sarcomas of the extremities (ESTSs) pose significant challenges in treatment and management due to their diverse nature and potential complications. This study aimed to assess complications associated with multimodal treatments involving surgery and radiotherapy (RT) and to identify potential risk factors. Methods: We retrospectively analyzed nonmetastatic ESTS patients treated with surgery and pre- or post-operative RT between 2007 and 2020 in Strasbourg, France. Complications, including wound complications (WCs), lymphedema, acute and chronic RT-related complications, and fractures, were meticulously evaluated. Results: A total of 169 patients diagnosed with localized ESTSs were included, with a median age of 64 years (range 21–94 years). ESTSs primarily occurred proximally (74.6%) and in the lower limbs (71%). The median follow-up was 5.5 years. WCs occurred in 22.5% of patients, with proximal and lower extremity tumors being significant risk factors. Acute RT-related complications included radiodermatitis, with grade ≥ 2 occurring in 43.1% of patients, which was associated with superficial tumors. Three patients had an edema grade ≥ 2. Chronic complications included telangiectasias (21.7%) and fibrosis (38.7%), with higher rates associated with larger PTVs and higher RT doses, respectively. Fractures occurred in 5 patients, mainly in the tibia (40%). Conclusions: Multimodal treatment of ESTSs demonstrated excellent tolerance, with manageable side effects. Numerous risk factors have been highlighted, providing insights for optimizing treatment strategies and enhancing patient care in this rare disease.
Enabling Navigation and Augmented Reality in the Sitting Position in Posterior Fossa Surgery Using Intraoperative Ultrasound
Authors: Bopp Miriam H. A. , Grote Alexander , Gjorgjevski Marko , Pojskic Mirza , Saß Benjamin , Nimsky Christopher
Published: May 2024

Despite its broad use in cranial and spinal surgery, navigation support and microscope-based augmented reality (AR) have not yet found their way into posterior fossa surgery in the sitting position. While this position offers surgical benefits, navigation accuracy and thereof the use of navigation itself seems limited. Intraoperative ultrasound (iUS) can be applied at any time during surgery, delivering real-time images that can be used for accuracy verification and navigation updates. Within this study, its applicability in the sitting position was assessed. Data from 15 patients with lesions within the posterior fossa who underwent magnetic resonance imaging (MRI)-based navigation-supported surgery in the sitting position were retrospectively analyzed using the standard reference array and new rigid image-based MRI-iUS co-registration. The navigation accuracy was evaluated based on the spatial overlap of the outlined lesions and the distance between the corresponding landmarks in both data sets, respectively. Image-based co-registration significantly improved (p < 0.001) the spatial overlap of the outlined lesion (0.42 ± 0.30 vs. 0.65 ± 0.23) and significantly reduced (p < 0.001) the distance between the corresponding landmarks (8.69 ± 6.23 mm vs. 3.19 ± 2.73 mm), allowing for the sufficient use of navigation and AR support. Navigated iUS can therefore serve as an easy-to-use tool to enable navigation support for posterior fossa surgery in the sitting position.
Relapsed/Refractory Diffuse Large B-Cell Lymphoma: Is There Still a Role for Autologous Stem Cell Transplantation in the CAR T-Cell Era?
Authors: Strüßmann Tim , Marks Reinhard , Wäsch Ralph
Published: May 2024

Recently, CD19-directed chimeric antigen receptor (CAR) T-cell therapies have revolutionized treatment strategies for diffuse large B-cell lymphoma (DLBCL). CAR T-cell therapy is increasingly used as a second-line therapy for patients with DLBCL with early relapse or refractoriness to initial chemoimmunotherapy and displaced high-dose chemotherapy, followed by autologous stem cell transplantation (ASCT) as the standard of care for these patients. However, patients with late relapse or chemosensitive disease still benefit from autologous stem cell transplantation. We will review practice-changing studies in early relapse (ZUMA-7 and TRANSFORM) under consideration of the negative BELINDA trial, with a focus on register data, comparing CAR T-cell therapy and ASCT for patients responding to salvage therapy.
Correction: Ruchaya et al. Transplantation of Skeletal Muscle-Derived Sca-1+/PW1+/Pax7− Interstitial Cells (PICs) Improves Cardiac Function and Attenuates Remodeling in Mice Subjected to Myocardial Infarction. Cells 2022, 11, 4050
Authors: Ruchaya Prashant J. , Lewis-McDougall Fiona C. , Sornkarn Nitiphat , Amin Sachin , Grimsdell Benjamin , Shaalan Abeer , Gritti Guilia , Soe Kyi Thar , Clark James E. , Ellison-Hughes Georgina M.
Published: May 2024

In the original publication [...]
Supporting Ageing Populations in Developing Countries: A Comparative Analysis of Pension Schemes and Policy Insights
Authors: Khai Tual Sawn , Oppong Nkansah Jacob , Khan Abdul Wali , Asaduzzaman Muhammad
Published: May 2024

The rapid growth of the elderly population is a major global demographic and social issue. Unfortunately, there is a shortage of pension plans and social security programmes for this population in developing countries, which has severe consequences for their quality of life and well-being. In this article, we aim to better understand the pension systems in developing country contexts such as Ghana, Pakistan, and Myanmar by reviewing official government materials (for example, pension reports) and the published literature to suggest relevant policy recommendations. We observed several policy implementation gaps and inequities in pension schemes for older people, specifically for informal and private sector workers. Considering the size of formal versus informal economies and the level of development index of each country, we suggest a wide variety of options for pension policies, financing, designing cash benefits, and pension payments to cover all older citizens. This article addresses the unmet needs of the elderly and their wider economic sustainability to ensure social justice and resource utilisation. Governments in developing countries should embrace and establish unique, inclusive, and friendly policies encompassing the informal sector to warrant older adults’ functional and social well-being with dignity and honour.
Understanding the Impact of Socioeconomic Factors on Early Childhood Development in Marginalised Roma Communities: The Role of Parental Education and Household Equipment
Authors: Plavnicka Jana , Chovan Shoshana , Filakovska Bobakova Daniela
Published: May 2024

This study aimed to explore the effect of socioeconomic disadvantage accumulated in marginalised Roma communities (MRCs) on early childhood development and to assess the role of selected socioeconomic indicators in the association between belonging to MRCs vs. the majority and early childhood development. We obtained cross-sectional data from 232 mother–child dyads from MRCs and the majority population. The differences in early childhood development and background variables between the two groups were tested using chi-square and Mann–Whitney U tests. The moderated mediation was tested using PROCESS Macro in SPSS Model 14 on 5000 bootstrap samples. Statistically significant differences between children from MRCs and the majority were found in terms of maternal age, parental education, household equipment, as well as early childhood development. Household equipment moderated the indirect effect of being from MRCs vs. the majority on early childhood development through parental education. The indirect effect through parental education was high at a low household equipment level, reduced at an average level and non-significant at a high level of household equipment. Our study uncovered disparities in early childhood development between children from MRCs and the majority population. Parental education significantly influenced developmental outcomes, while household equipment mitigated its impact.
Identification of Key Hypolipidemic Components and Exploration of the Potential Mechanism of Total Flavonoids from Rosa sterilis Based on Network Pharmacology, Molecular Docking, and Zebrafish Experiment
Authors: Wu Boxiao , Li Churan , Luo Xulu , Kan Huan , Li Yonghe , Zhang Yingjun , Rao Xiaoping , Zhao Ping , Liu Yun
Published: May 2024

Hyperlipidemia is a prevalent chronic metabolic disease that severely affects human health. Currently, commonly used clinical therapeutic drugs are prone to drug dependence and toxic side effects. Dietary intervention for treating chronic metabolic diseases has received widespread attention. Rosa sterilis is a characteristic fruit tree in China whose fruits are rich in flavonoids, which have been shown to have a therapeutic effect on hyperlipidemia; however, their exact molecular mechanism of action remains unclear. Therefore, this study aimed to investigate the therapeutic effects of R. sterilis total flavonoid extract (RS) on hyperlipidemia and its possible mechanisms. A hyperlipidemic zebrafish model was established using egg yolk powder and then treated with RS to observe changes in the integral optical density in the tail vessels. Network pharmacology and molecular docking were used to investigate the potential mechanism of action of RS for the treatment of hyperlipidemia. The results showed that RS exhibited favorable hypolipidemic effects on zebrafish in the concentration range of 3.0–30.0 μg/mL in a dose-dependent manner. Topological and molecular docking analyses identified HSP90AA1, PPARA, and MMP9 as key targets for hypolipidemic effects, which were exerted mainly through lipolytic regulation of adipocytes and lipids; pathway analysis revealed enrichment in atherosclerosis, chemical carcinogenic-receptor activation pathways in cancers, and proteoglycans in prostate cancer and other cancers. Mover, chinensinaphthol possessed higher content and better target binding ability, which suggested that chinensinaphthol might be an important component of RS with hypolipidemic active function. These findings provide a direction for further research on RS interventions for the treatment of hyperlipidemia.
Genetics and Epigenetics in Acquired Hemophilia A: From Bench to Bedside
Authors: Evangelidis Nikolaos , Kotsiou Nikolaos , Evangelidis Paschalis , Alevizopoulos Vlasios I. , Dermitzakis Iasonas , Chissan Sofia , Vakalopoulou Sofia , Gavriilaki Eleni
Published: May 2024

Acquired hemophilia A (AHA) is a bleeding disorder characterized by the immunological inhibition of factor VIII (FVIII) of the hemostatic pathway leading to hemorrhagic events. Different domains of FVIII are the target of autoantibodies (mainly immunoglobulin (Ig) G) leading to the deficiency of FVIII. Several factors have been associated with the activation of the auto-immunity towards FVIII. Emerging evidence implicates CD4+ T cell activation in mediating this autoimmune response, with their involvement like that observed in congenital hemophilia A. Several genes such as HLA II DRB*16, DQB1*0502, and CTLA-4 + 49 are responsible for the pathogenesis of AHA. Epigenetic modifications and mainly long-coding RNAS (lncRNAs) are potentially contributing to the pathogenesis of AHA. The treatment approach of AHA includes the management of acute bleeding events and the administration of immunosuppressive medications. This review aimed to summarize the published data on the genetics and epigenetics of AHA. The severity and the mortality of this disease are creating an emerging need for further research in the field of the genetics and epigenetics of acquired hemorrhagic disorder.
RETRACTED: Sun et al. Study on the Removal Efficiency and Mechanism of Tetracycline in Water Using Biochar and Magnetic Biochar. Coatings 2021, 11, 1354
Authors: Sun Hongwei , Yang Jingjie , Wang Yue , Liu Yucan , Cai Chenjian , Davarpanah Afshin
Published: May 2024

The Coatings Editorial Office retracts the article entitled “Study on the Removal Efficiency and Mechanism of Tetracycline in Water Using Biochar and Magnetic Biochar” [...]
The Impact of Bonding Agents and Bone Defects on the Fracture Resistance of Reattached Vertically Root-Fractured Teeth
Authors: Haralur Satheesh B. , Alshahrani Nasser Mohammed , Alafra Saeed Hadi , Hakami Muath Ali , AbuMesmar Omar Abdulaziz , Al-Qarni Mohammed A. , AlQahtani Saeed M. , Alqahtani Nasser M.
Published: May 2024

Many patients experience vertical root fractures, and clinicians often consider conservative treatment options like reattaching the fractured root segments. The study investigated the impact of different bonding agents on the fracture resistance of rebonded vertically fractured teeth with various alveolar bone defects. Human premolar teeth with a single root were sectioned and reattached using dual-cure resin cement (DCRC), resin-modified glass ionomer (RMGI), and cyanoacrylate. The reattached teeth were then restored with a resin fiber post, composite resin core, and full veneer metal copings. These teeth were embedded in acrylic blocks with angular, V-shaped, and step-shaped bone defects to simulate various alveolar bone conditions. After subjecting the samples to thermal cycling, the fracture resistance was evaluated using a universal testing machine. Teeth samples reattached with RMGI exhibited a higher average fracture resistance. The study also found that DCRC proved to be an effective bonding agent for VRF teeth. However, cyanoacrylate-rebonded teeth exhibited the lowest fracture resistance. The V-shaped defects had a significant impact on the fracture resistance of reattached VRF teeth, with largely unfavorable fractures observed in these cases. Predominantly favorable fractures were observed in the teeth treated with RMGI. The fracture loads in both RMGI and DCRC groups exceeded the expected masticatory load.
Does “Paper Oil” Matter? Energy Markets’ Financialization and Co-Movements with Equity Markets
Authors: Büyükşahin Bahattin , Robe Michel A.
Published: May 2024

We revisit, and document new facts regarding, the financialization of U.S. energy markets in 2000–2010. We show that, after controlling for macroeconomic factors and physical energy market fundamentals, the strength of energy markets’ co-movements with the U.S. stock market is positively related to the energy paper market activity of hedge funds that trade both asset classes. This relation weakens when credit risk is elevated. We find, in contrast, no link with the aggregate positions of commodity index traders in energy futures markets. Our findings have implications for the ongoing debate regarding the financialization of commodities.
Robust Algorithms for the Analysis of Fast-Field-Cycling Nuclear Magnetic Resonance Dispersion Curves
Authors: Bortolotti Villiam , Conte Pellegrino , Landi Germana , Lo Meo Paolo , Nagmutdinova Anastasiia , Spinelli Giovanni Vito , Zama Fabiana
Published: May 2024

Fast-Field-Cycling (FFC) Nuclear Magnetic Resonance (NMR) relaxometry is a powerful, non-destructive magnetic resonance technique that enables, among other things, the investigation of slow molecular dynamics at low magnetic field intensities. FFC-NMR relaxometry measurements provide insight into molecular motion across various timescales within a single experiment. This study focuses on a model-free approach, representing the NMRD profile R1 as a linear combination of Lorentzian functions, thereby addressing the challenges of fitting data within an ill-conditioned linear least-squares framework. Tackling this problem, we present a comprehensive review and experimental validation of three regularization approaches to implement the model-free approach to analyzing NMRD profiles. These include (1) MF-UPen, utilizing locally adapted L2 regularization; (2) MF-L1, based on L1 penalties; and (3) a hybrid approach combining locally adapted L2 and global L1 penalties. Each method’s regularization parameters are determined automatically according to the Balancing and Uniform Penalty principles. Our contributions include the implementation and experimental validation of the MF-UPen and MF-MUPen algorithms, and the development of a “dispersion analysis” technique to assess the existence range of the estimated parameters. The objective of this work is to delineate the variance in fit quality and correlation time distribution yielded by each algorithm, thus broadening the set of software tools for the analysis of sample structures in FFC-NMR studies. The findings underline the efficacy and applicability of these algorithms in the analysis of NMRD profiles from samples representing different potential scenarios.
Constrained Device Performance Benchmarking with the Implementation of Post-Quantum Cryptography
Authors: Fitzgibbon Gregory , Ottaviani Carlo
Published: May 2024

Advances in quantum computers may pose a significant threat to existing public-key encryption methods, which are crucial to the current infrastructure of cyber security. Both RSA and ECDSA, the two most widely used security algorithms today, may be (in principle) solved by the Shor algorithm in polynomial time due to its ability to efficiently solve the discrete logarithm problem, potentially making present infrastructures insecure against a quantum attack. The National Institute of Standards and Technology (NIST) reacted with the post-quantum cryptography (PQC) standardization process to develop and optimize a series of post-quantum algorithms (PQAs) based on difficult mathematical problems that are not susceptible to being solved by Shor’s algorithm. Whilst high-powered computers can run these PQAs efficiently, further work is needed to investigate and benchmark the performance of these algorithms on lower-powered (constrained) devices and the ease with which they may be integrated into existing protocols such as TLS. This paper provides quantitative benchmark and handshake performance data for the most recently selected PQAs from NIST, tested on a Raspberry Pi 4 device to simulate today’s IoT (Internet of Things) devices, and provides quantitative comparisons with previous benchmarking data on a range of constrained systems. CRYSTALS-Kyber and CRYSTALS-Dilithium are shown to be the most efficient PQAs in the key encapsulation and signature algorithms, respectively, with Falcon providing the optimal TLS handshake size.
Experimental and Modelling Research on the Effect of Prior Ferrite on Bainitic Transformation in Medium-Carbon Bainitic Steel
Authors: Yu Xinpan , Liu Wei , He Kang , Wang Tengfei , Niu Gang , Wu Huibin
Published: May 2024

In this study, we investigate the impact of prior ferrite on the bainite transformation kinetics and microstructure of medium-carbon steel interrupted by an intercritical annealing (IAA) process. It was found that the incubation time and completion time decreased from 687 s and 6018 s to 20 s and 4680 s, with the volume fraction of ferrite increasing from 9.5% to 28.6%, while the maximum transformation rate increased from 00271 μm/s to 0.0436 μm/s. The ferrite/austenite interface is introduced, and the nucleation sites are increased to accelerate the subsequent bainite transformation due to the formation of prior ferrite. However, there is a competitive relationship between the number and activation energy of bainite nucleation. According to the experimental results and theoretical calculations, the activation energy of the bainite transformation in the medium-carbon bainite steel decreases gradually with an increase in the volume fraction of prior ferrite.
Promoting Thermal Conductivity of Alumina-Based Composite Materials by Systematically Incorporating Modified Graphene Oxide
Authors: Lee Nawon , Park Jinsol , Jang Nayeon , Lee Sehui , Kim Dayeon , Yun Sanggin , Park Tae Woo , Kim Jun-Hyun , Park Hyun-Ho
Published: May 2024

Small amounts of thermally conductive graphene oxide (GO) and modified GO are systematically introduced as a second filler to thermal interface materials (TIMs) consisting of alumina (Al2O3) particles and polydimethylsiloxane (PDMS). The surface of GO is covalently linked with an organic moiety, octadecylamine (ODA), to significantly improve the miscibility and dispersity of GO across the TIM matrix. Subsequently, two series of PDMS-Al2O3 composite TIMs are manufactured as a function of GO and ODA-GO content (0.25 wt%–2.5 wt%) to understand the effect of these second additives. The incorporation of GO into the Al2O3-PDMS composite materials generally increases the thermal conductivity (TC), ranging from 18% to 29%. Conversely, the use of ODA-GO further enhances the overall performance of TIMs (22–54%) by facilitating the dispersion degree of GO across the composite matrix. The great improvement in TC is presumably related to the formation of conductive pathways by uniformly integrating 2D-type GO flakes across spherical Al2O3 particle networks. The ability to simply regulate the polarity of the thermally conductive second filler can provide an idea for designing cost-effective and practical TIM-2-type pads that can be commercially applicable in between an integrated heat spreader and a heat sink.
Genetic Diversity and Relationships among Indian Jujube (Ziziphus mauritiana Lamk.) Cultivars Using Morphometric Characteristics, matK Barcoding, and ISSR Markers
Authors: Abdel-Sattar Mahmoud , Al-Obeed Rashid S. , Rihan Hail Z. , El-Badan Ghada E.
Published: May 2024

Indian jujube displays genetic diversity and does not prominently display minute morphometric variations, and this makes correct identification a difficult and long-term task. However, little work has been conducted to bring jujube cultivars into domestication. So, the present study aimed to evaluate eleven cultivars of Indian jujube in terms of the fruit’s morphometric characteristics, as well as molecular marker studies by plastidial megakaryocyte-associated tyrosine kinase (matK) barcoding and inter-simple sequence repeats (ISSR) markers for species differentiation, identification, and relationships among Indian jujube cultivars. The results of the morphometric characteristics showed that the mean geometric diameter, surface area, sphericity, sphericity ratio, shape index, fruit length, fruit diameter, fruit weight, and seed weight varied among cultivars. The results also showed that the color values of L*, a*, and b* for fruits differed in different cultivars. In addition, the results showed a discrepancy in the genetic diversity parameters related to the matK barcoding, ISSR markers, and relationships among Indian jujube cultivars. Substantially, hierarchical clustering by heatmap revealed that ‘Zytoni’ and ‘Um-Sulaem’ with spines seem to be mono-clades distinct from other cultivars, which related to variations in the expression levels of genes. Therefore, they should be relied upon together to distinguish and identify cultivars in order to maximize the effectiveness of local germplasm conservation and exploitation.
Uterine Arteriovenous Malformation: Diagnostic and Therapeutic Challenges
Authors: Clavero Bertomeu Luisa , Castro Portillo Laura , Fernández-Conde de Paz Cristina
Published: May 2024

Uterine arteriovenous malformations are a rare cause of puerperal haemorrhage, but their incidence is increasing due to both improved diagnosis and the more frequent use of uterine surgery in recent years. The use of ultrasound, both B-mode and Doppler, is recommended for diagnosis and follow-up, as it has been shown to be the simplest and most cost-effective method. Endometrial thickening associated with an anechoic and vascular intramiometrial structure is very useful for diagnosis and can help to exclude other causes of dysfunctional bleeding. Pulsed Doppler shows low-resistance vessels and high pulsatility indices with a high peak systolic velocity (PSV). In a healthy myometrium, the vessels have a peak systolic velocity of 9–40 cm/s and a resistance index between 0.6 and 0.8, whereas in the case of AVMs, the systolic and diastolic velocities are 4–6 times higher (PSV 25–110 cm/s with a mean of 60 cm/s and a resistance index of 0.27–0.75 with a mean of 0.41). For treatment, we must individualise each case, taking into account haemodynamic stability, the patient’s reproductive wishes, and the severity of the AVM as assessed by its size and PSV.
Prognostic Factors Associated with Breast Cancer-Specific Survival from 1995 to 2022: A Systematic Review and Meta-Analysis of 1,386,663 Cases from 30 Countries
Authors: Abdul Rahman Hanif , Zaim Siti Nurzaimah Nazhirah , Suhaimei Ummi Salwa , Jamain Al Amin
Published: May 2024

Breast cancer is the fifth-ranked cancer globally. Despite early diagnosis and advances in treatment, breast cancer mortality is increasing. This meta-analysis aims to examine all possible prognostic factors that improve/deteriorate breast cancer-specific survival. MEDLINE, PubMed, ScienceDirect, Ovid, and Google Scholar were systematically searched until September 16, 2023. The retrieved studies from 1995 to 2022 accumulated 1,386,663 cases from 30 countries. A total of 13 out of 22 prognostic factors were significantly associated with breast cancer-specific survival. A random-effects model provided a pooled estimate of the top five poorest prognostic factors, including Stage 4 (HR = 12.12; 95% CI: 5.70, 25.76), followed by Stage 3 (HR = 3.42, 95% CI: 2.51, 4.67), a comorbidity index ≥ 3 (HR = 3.29; 95% CI: 4.52, 7.35), the poor differentiation of cancer cell histology (HR = 2.43; 95% CI: 1.79, 3.30), and undifferentiated cancer cell histology (HR = 2.24; 95% CI: 1.66, 3.01). Other survival-reducing factors include positive nodes, age, race, HER2-receptor positivity, and overweight/obesity. The top five best prognostic factors include different types of mastectomies and breast-conserving therapies (HR = 0.56; 95% CI: 0.44, 0.70), medullary histology (HR = 0.62; 95% CI: 0.53, 0.72), higher education (HR = 0.72; 95% CI: 0.68, 0.77), and a positive estrogen receptor status (HR = 0.78; 95% CI: 0.65, 0.94). Heterogeneity was observed in most studies. Data from developing countries are still scarce.