bluuebunny/crossref_metadata_2025 huggingface dataset:
Mechanism and Experimental Investigation of Vibration Reduction for Container Crane Based on Particle Damping Technology
Authors: Ye Fangping , Xue Xinyi , Jiang Weijie , Yin Xiyan
Published: December 2023

Container crane has been widely used for port operation. However, the structure of port container crane is large, which always lead to crane damage under the vibration and strong wind. Therefore, a method for vibration reduction of container crane structure based on particle damping technology is proposed. In this investigation, the scale 1:80 crane model is built, the equivalent mechanical model is also established to preliminary verify the effect of vibration suppression. Furthermore, the dependence of vibration suppression effect on the key parameters of the damper, i.e., filling material, filling rate, particle size, and installation position of dampers are analyzed through experiments. The results indicate that the vibration peak of the crane structure displayed in Simulink oscilloscope is weakened and lags behind, the installation of the damper brings the effect of vibration suppression for the crane, the vibration suppression effect reaches 25%; the crane mode obtains the best vibration suppression effect under the condition that the material filled in the damper has a large density and elastic modulus, sufficient collision space and high collision frequency, and the dampers are installed far away from the crane center of gravity. The optimum parameters of the damper obtained from experiments, 12 mm lead beads, filling rate of 60%, installed in the distal ends within the strength range of the forearm of the crane. It is thus concluded that the particle damping technology has provided an effective way in wind and vibration reduction for container crane.
Late presentation of Ebstein Anomaly: A Unique Case at 67yrs
Authors: Roy Ishita , Roy Nilanjan
Published: December 2023

Ebstein anomaly, a congenital heart condition, can manifest across a broad age spectrum, including adulthood. We present a unique case of a 67-year-old woman with no significant medical history who sought medical attention for severe headaches, vomiting, and respiratory distress, accompanied by elevated blood pressure and rapid pulse. Initial investigations, including troponin T levels, yielded unremarkable results. However, an electrocardiogram unveiled a right bundle branch block (RBBB) pattern, prompting further assessment. A subsequent chest X-ray disclosed an enlarged heart, indicating cardiomegaly, prompting echocardiography. The echocardiogram confirmed the diagnosis of Ebstein anomaly, revealing a 26 mm apical displacement of the tricuspid valve (TV). The patient was managed conservatively with supplemental oxygen and exhibited gradual improvement. This case underscores the infrequent occurrence of Ebstein anomaly in adulthood and underscores the necessity for thorough evaluation in cases of unexplained cardiopulmonary symptoms.
Late presentation of Ebstein Anomaly: An Unique Case at 67yrs
Authors: Roy Ishita , Roy Nilanjan
Published: December 2023

Ebstein anomaly, a congenital heart condition, can manifest across a broad age spectrum, including adulthood. We present a unique case of a 67-year-old woman with no significant medical history who sought medical attention for severe headaches, vomiting, and respiratory distress, accompanied by elevated blood pressure and rapid pulse. Initial investigations, including troponin T levels, yielded unremarkable results. However, an electrocardiogram unveiled a right bundle branch block (RBBB) pattern, prompting further assessment. A subsequent chest X-ray disclosed an enlarged heart, indicating cardiomegaly, prompting echocardiography. The echocardiogram confirmed the diagnosis of Ebstein anomaly, revealing a 26 mm apical displacement of the tricuspid valve (TV). The patient was managed conservatively with supplemental oxygen and exhibited gradual improvement. This case underscores the infrequent occurrence of Ebstein anomaly in adulthood and underscores the necessity for thorough evaluation in cases of unexplained cardiopulmonary symptoms.
Mortality Trends in People With Disabilities Before and During the COVID-19 Pandemic in South Korea, 2017–2022
Authors: Kim¹ Ye-Soon , Kim¹ Ju-Hee , Kwon¹ Sooyoung , Kim¹ Joo-Hee , Kim¹ Hyun-Ji , Ho¹ Seung Hee
Published: December 2023

Conclusions: The mortality rate of people with disabilities from all causes did not significantly increase compared to the pre-COVID-19 era. However, COVID-related deaths among people with disabilities increased significantly.
Prevalence and Predictive Factors of Zinc Deficiency in Orthopaedic Inpatients
Authors: Iida Daisuke , Shigemura Tomonori , Yamamoto Yohei , Murata Yasuaki
Published: December 2023

Zinc, an essential trace mineral, plays a crucial role in over 300 enzymes that are vital for major metabolic pathways. Zinc deficiency can cause various symptoms and sometimes lead to serious conditions, especially after surgery. Therefore, recognising the prevalence and predictors of zinc deficiency before surgery is essential for preventing associated symptoms. Although zinc deficiency is linked to several diseases, its association with orthopaedic disorders remains unclear. This retrospective study aimed to explore the prevalence and predictors of zinc deficiency in orthopaedic inpatients prior to surgery. This retrospective case-control study aimed to investigate the prevalence and predictors of zinc deficiency in orthopaedic inpatients. Patients admitted to the Department of Orthopaedic Surgery at Teikyo University Chiba Medical Center between 15 February 2022 and 31 August 2022 were included. The case-control design categorised patients into zinc deficiency (case) and non-deficiency (control) groups based on serum zinc concentrations (< 60 µg/dL). The data retrieved from the centre’s database included demographics, comorbidities, reasons for hospitalisation, fracture details, medication use, and laboratory values. Statistical tests included Fisher’s exact test for categorical variables and a two-sample t-test for continuous vari-ables. To identify factors associated with zinc deficiency, multivariate analysis ( backward stepwise regression model) was performed for items that were significantly different from the univariate analysis. A total of 156 orthopaedic patients were included in this study. Forty-seven patients (30.1%) had a zinc deficiency. The fracture rate was significantly higher in the case group than in the control group (68.1 % vs. 33.9 %; p < 0.001); spinal disease was significantly less common in the case group than in the control group (2.1 % vs. 31.2 %; p < 0.001); and OA was significantly less common in the case group than in the control group (8.5 % vs. 22.9 %; p = 0.04). The case group had signif-icantly higher rates of fragility and hip and pelvic fractures than did the control group. Spinal disease was less common in the case group than in the control group. Laboratory findings revealed significantly lower mean serum zinc (46.2 µg/dL) in the case group than in the control group (76.3 µg/dL), accompanied by lower albumin and haemoglobin concentrations. Hypoalbuminemia and anaemia were more prevalent in the case group than in the control group. Multivariate logistic regression identified age ≥ 60, anaemia, hip fracture, and hypoalbuminaemia as independent predictive factors for zinc deficiency, while spinal disease was associated with decreased risk. This study identified zinc deficiency in 30.1% of orthopaedic inpatients based on serum zinc concen-trations. Furthermore, age ≥ 60, anaemia, hip fracture, and hypoalbuminaemia were determined as independent predictive factors for zinc deficiency.
Comparison of Cost Analysis in Patients with Tetrahydrobiopterin-Responsive and Non-responsive Phenylketonuria
Authors: Karaca Sahin Merve , Aktuglu Zeybek Ayse Cigdem , Zubarioglu Tanyel , Cansever Mehmet Serif , Kıykım Ertuğrul
Published: December 2023

Phenylketonuria is an inherited metabolic disorder that leads to neurobehavioral dysfunction. The main treatment is a low-phenylalanine diet and/or the cofactor tetrahydrobiopterin. Regular outpatient follow-up care and measurement of the phenylalanine level in the blood are required. We aimed to analyze the economic burden of phenylketonuria on families and the state. The pa-tients with phenylketonuria were divided into three groups according to their treatment: a low-phenylalanine diet group (n= 50), a tetrahydrobiopterin group (n= 44) and a group taking tetra-hydrobiopterin together with the diet (n= 25). A comparative cost analysis was carried out. The annual economic burden to the state was calculated to average €18801±15345 and was lowest in the diet group, then in the tetrahydrobiopterin group and highest in the tetrahydrobiopterin+diet group (p < 0.001). Out-of-pocket costs amounted to €1531±1173 per year, and indirect losses aver-aged €2125±1930 per year for all families. The economic loss was significantly lower in the fami-lies taking tetrahydrobiopterin than in the other groups (p=0.001). The combined use of medical nutrition and BH4 treatments has been shown to increase the economic harm to the state. Reim-bursing low-protein products and increasing the rate of patients eligible for financial allowances may reduce the economic harm to families.
Monoketone Curcuminoids: An Updated Review of Their Synthesis and Biological Activities
Authors: Vieira Tatiana M. Manzini , Tanajura Lívia Stenico , Heleno Vladimir Constantino Gomes , Magalhães Lizandra Guidi , Crotti Antônio Eduardo Miller
Published: December 2023

Curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadien-3,5-dione], a component of Curcuma longa L. rhizomes, displays various biological and pharmacological activities. However, it is poorly bioavailable and unstable in physiological pH, which has led more stable and effective curcumin analogs (e.g., monoketone curcuminoids, or MKCs) to be synthesized, and their biological activities to be described. In this review, we cover papers published between 2019 and 2023 on the antimicrobial, anticancer, antioxidant, and antiparasitic actions as well as other less common MKC biological and pharmacological activities. We also address the Claisen-Schmidt condensation as a standard procedure to synthesize MKCs.
Navigating Uncertainty: Enhancing Markowitz Asset Allocation Strategies through Out-of-Sample Analysis
Authors: Kanaparthi Vijaya Krishna
Published: December 2023

This paper elucidates the impact of uncertainty on the Markowitz asset allocation and how it performs. The findings imply that when evaluated out-of-sample, estimation errors in parameters might significantly affect how well an allocation performs. Numerous publications that address this ambiguity have been highlighted to emphasize our findings further. In our work, we compare these approaches to alternative allocation strategies and explain their performance in both expected and real out-of-sample events. We find that the Markowitz framework can be improved by using tactics that take uncertainty into account. Longer sample numbers, however, may not always translate into better outcomes. Applying a short-sale constraint can also enhance the initial portfolio. Finally, we find that even more basic approaches to asset allocation, such as equally weighted allocation, perform reasonably well.
Pre-analytical Considerations for Clinical Assays of Circulating Human miRNA-451a for Diagnostic Purposes.
Authors: Fernando M. Rohan , Chandel Dinesh S , Tom Wesley , Jiang Chao , Krzyzanowski Gary , Fernando Nirmalee
Published: December 2023

Circulating miRNA-451a has recently emerged as an important biomolecule with potential clinical values as a diagnostic marker for several diseases. However, to be used as such, it is critical to accurately assay miRNA-451a in the clinic. Yet, pre-analytical factors that can affect such an error-free clinical assay of miRNA-451a have not been explored. This study aimed at investigating several of these pre-analytical factors that may affect the accurate quantification of miRNA-451a in human blood samples. We initially evaluated miRNA-451a levels in red blood cells (RBCs), white blood cells (WBCs), platelets, and plasma by droplet digital PCR (ddPCR). Next, we quantified miRNA-451a levels in whole blood or plasma stored at different temperatures for different time periods by ddPCR and used hemolyzed plasma samples to study the effects of hemolysis on plasma miRNA-451a concentration. We found that WBCs and platelets contain trace amounts of miRNA-451a and that about 99.9% of miRNA-451a in the blood are localized in RBCs. Hemolyzed plasma showed 58.5-fold increase in miRNA-451 concentration as compared to non-hemolyzed plasma. Importantly, plasma stored at room temperature (RT) or 4ºC showed a steady statistically significant decrease in miRNA-451a levels over a 7-day period whereas plasma samples stored at -20 or -80ºC for up to 7 days showed stable miRNA-451a levels. Together, our data indicate that hemolysis and blood storage at RT or 4ºC may have significant negative effects on the levels of circulating miRNA-451a.
Sustainable Economy: Eco-Branding of an Industrial Region of Kazakhstan
Authors: Davidenko Lyudmila Mikhailovna , Sherimova Nurzhanat Mukhtarovna , Kunyazova Saule Kairbekovna , Amirova Maral Akbaevna , Beisembina Ansagan Nygmetovna
Published: December 2023

In the sustainable economy, consumer preferences are gradually coming to prioritise environmentally friendly products and services. A sustainable economy is directly linked to the growth of consumer welfare and environmental culture. The success of environmental projects is largely determined by approaches to the management of complex interconnected objects. Technological integration and the value chain from raw material extraction to the marketing of environmentally friendly products add to the growth factors. In this regard, we formulate the purpose of this study: to generalise the approaches and improve the mechanism of the ecological branding of the industrial complex of a region through conscious ESG transformation. For this purpose, we studied statistics and principles of management systems of Kazakh companies and their stakeholders. We analysed the situation of industrial complexes and their partners in the external environment to identify barriers to the development and implementation of eco-branding tools. The theoretical significance of the study helps to reveal the influence of ecological principles of modern production organisation on the speed of transition to green technologies. Its practical significance is seen in the formation of a system for measuring the level of readiness of companies to promote the ecological branding of a region’s industry outside its country.
Assessing the Influence of Fat Content upon the Sensory and Textural Properties of Margarine to Guide the Development of Healthier Products
Authors: BOLINI HELENA MARIA ANDRE , Medeiros Alessandra Cazellatto , Pereira Cecília Teresa Muniz , Carraro Francisco , Augusto Pedro Pio Campregher , Cardello Flavio , Lima Rafael Sousa
Published: December 2023

Margarine exhibits significant variations in composition, allowing it to cater to diverse consumer segments. This study aimed to characterize the physical and sensory attributes of margarine samples available in the Brazilian market. Twelve commercial samples from six different brands, encompassing 30% to 80% of lipid contents, were subjected to instrumental texture analysis, sensory profiling, and hedonic assessment. A total of 112 consumers participated in acceptance tests and Check-All-That-Apply (CATA) evaluations, while another group of 62 subjects performed Projective Mapping. Samples with lipid percentages exceeding 70% achieved the highest average acceptance scores for taste and overall impression. The brand with the lowest lipid content (30%) exhibited a stronger association with negative attributes, including rancid flavor and aroma, bitterness, and metallic flavor, resulting in lower average scores for aroma, flavor, texture, and overall impression. However, these scores were not statistically different from those of samples with 50% and 60% lipid content. Reducing lipid levels in fat-based products such as margarine poses a challenge to food manufacturers, as consumers generally perceive higher lipid percentages as indicative of superior flavor quality.
Redox-Active Cerium Fluoride Nanoparticles Mitigate of Double-Stranded DNA Breakage and Modulate Gene Expression Due to Radiation Exposure In Vitro
Authors: Chukavin Nikita N. , Fillipova Kristina O. , Ermakov Artem M. , Karmanova Ekaterina E. , Popova Nelli R. , Anikina Viktoriia A. , Ivanova Olga S. , Ivanov Vladimir K. , Popov Anton L.
Published: December 2023

Ionizing radiation-induced damage in cancer and normal cells leads to apoptosis and cell death, through the intracellular oxidative stress, DNA damage and disorders of their metabolism. Irradiation doses that do not lead to the death of tumor cells can result in the emergence of radioresistant clones of these cells, due to the rearrangement of metabolism and the emergence of new mutations, including those in the genes responsible for DNA repair. The search for the substances capable of modulating the functioning of the tumor cell repair system is an urgent task. Here we analyzed the effect of cerium(III) fluoride nanoparticles (CeF3 NPs) on normal (human mesenchymal stem cells – hMSCs) and cancer (MCF-7 line) human cells after X-ray radiation. CeF3 NPs effectively prevent the formation of hydrogen peroxide and hydroxyl radicals in an irradiated aqueous solution, showing pronounced antioxidant properties. CeF3 NPs are able to protect hMSCs from radiation-induced proliferation arrest, increasing their viability and mitochondrial membrane potential, and, conversely, inducing the cell death of MCF-7 cancer cells, causing radiation-induced mitochondrial hyperpolarization. CeF3 NPs provided a significant decrease in the number of double-strand breaks (DSBs) in normal cells, while in MCF-7 cells the number of γ-H2AX foci dramatically increased in the presence of CeF3 4 hours after irradiation. In the presence of CeF3 NPs, there was a tendency to modulate the expression of most analyzed genes associated with the development of intracellular oxidative stress, cell redox status and the DNA repair system after X ray irradiation. Cerium-containing nanoparticles are capable of providing selective protection of normal cells from radiation-induced injuries and are considered as a platform for the development of promising clinical radioprotectors.
Nonparametric Partial Linear Estimation for Spatial Functional Data with Missing At-Random
Authors: Benchikh Tawfik , Almanjahie Ibrahim M. , Fetitah Omar , Attouch Mohammed kadi
Published: December 2023

The aim of this paper is to study a semi-functional partial linear regression model (SFPLR) for spatial data with responses missing at random. The estimators are constructed by the kernel method, and some asymptotic properties such as probability convergence rates of the nonparametric component and asymptotic distribution of the parametric and nonparametric components are established under certain conditions. Next, the performances and the superiority of these estimators are presented and examined using a study on simulated data and on real data by carrying out a comparison between our semi-functional partially linear model with MAR estimator (SFPLRM), the semi-functional partially linear model with the full-case estimator (SFPLRC) and the nonparametric functional model estimator with MAR (FNPM). The results show that the proposed estimators outperform existing estimators as the number of random missing data increases.
Mapping Soil Organic Carbon Stock and Uncertainties in an Alpine Valley (Northern Italy) Using Machine Learning Models
Authors: Agaba Sara , Ferre Chiara , Musetti Marco , Comolli Roberto
Published: December 2023

In this study, we conducted a comprehensive analysis of the spatial distribution of soil organic carbon stock (SOC stock) and the associated uncertainties in two soil layers (0–10 cm and 0–30 cm; SOC stock 10 and SOC stock 30 respectively), in Valchiavenna, an alpine valley located in northern Italy . We employed the digital soil mapping (DSM) approach within different machine learning models, including multivariate adaptive regression splines (MARS), random forest (RF), support vector regression (SVR), and elastic net (ENET). Our dataset comprised soil data from 110 profiles, with SOC stock calculations for all sampling points based on bulk density (BD), whether measured or estimated, considering the presence of rock fragments. As environmental covariates for our research we utilized environmental variables, in particular geomorphometric parameters derived from a digital elevation model (with a 20 m pixel resolution), land cover data, and climat-ic maps. To evaluate the effectiveness of our models, we evaluated their capacity to predict SOC stock 10 and SOC stock 30 using the coefficient of determination (R2). The results for the SOC stock 10 were as follows: MARS 0.39, ENET 0.41, RF 0.69, and SVR 0.50. For the SOC stock 30, the corresponding R2 values were: MARS 0.45, ENET 0.48, RF 0.65, and SVR 0.62. Additionally, we calculated the Root Mean Square Error (RMSE) and Mean Absolute Error (MAE) for further assessment. To map the spatial distribution of SOC stock and address uncertainties in both soil layers, we chose the RF model, due to its better performance, as indicated by the highest R2 and the lowest RMSE and MAE. The resulting SOC stock maps using the RF model demonstrated an accuracy of RMSE = 1.35 kg.m-2 for the SOC stock 10 and RMSE= 3.36 kg.m-2 for the SOC stock 30. To further evaluate and illustrate the precision of our soil maps, we conducted an uncertainty assessment and mapping by analyzing the standard deviation (SD) from 50 iterations of the best-performing RF model. This analysis effectively highlighted the high accuracy achieved in our soil maps. The maps of uncertainty demonstrated that the RF model better predicts the SOC stock 10 compared to the SOC stock 30.
Switching from VA-ECMO to a Percutaneous Right Ventricular Assist Device as a Bridge to Recovery in a Case of Right Ventricular Myocardial Infarction
Authors: Assouline Benjamin , Mugnai Damiano , Hagerman Andres , Wuarin Lydia , Schopfer Léonore , Bendjelid Karim , Giraud Raphaël
Published: December 2023

Background
Acute right heart failure (RHF) is associated with high morbidity and mortality. Temporary mechanical circulatory support (tMCS) is now part of the advanced management strategy for refractory cardiogenic shock (CS). Among such support systems, VA-ECMO remains the fastest MCS system to set up. The ProtekDuo cannula can be used as a temporary and percutaneous right ventricular assist device (RVAD) in relay with VA-ECMO in the context of prolonged weaning.
Case Summary
This case report describes the clinical course of a 69-year-old patient who underwent elective aortic valve replacement surgery complicated by acute RHF due to iatrogenic ligation of the right coronary artery. VA-ECMO was initiated in the context of refractory cardiogenic shock. Faced with prolonged MCS weaning, due to lack of recovery of the right ventricle associated with the absence of any other therapeutic option, a percutaneous RVAD via a ProtekDuo cannula was implanted as a bridge to recovery. The ProtekDuo was gradually weaned while the patient began rehabilitation. The patient was transferred to the medical ward after 5 weeks in the ICU, stayed 5 months in an acute medical setting, went to a rehabilitation center afterward and returned home 6 months after the initial surgery without any neurological sequalae or organ failures. In total, she remained on MCS for 39 days (21 on VA-ECMO and 18 on ProtekDuo).

Discussion
In cases of refractory RHF, after stabilization and organ perfusion restoration under VA-ECMO, it is feasible to switch to a percutaneous RVAD, allowing early mobilization of patients during prolonged MCS weaning until the right ventricle adapts to new loading conditions.
Investigation on the Performance of Battery Thermal Management Based Direct Refrigerant Cooling: Simulation, Validation of Results and Parametric Studies
Authors: Jamsawang Suparat , Chanthanumataporn Saharat , Sutthivirode Kittiwoot , Thongtip Tongchana
Published: December 2023

This paper proposes a simulation technique for investigating the battery thermal management system based direct refrigerant cooling (BTMS-DRC). It employs finite element method for a combined-conduction-convection heat transfer to predict the module temperature and to prove the temperature uniformity. The refrigerant side cooling is based on the two-phase flow evaporation which is represented by the convection heat transfer (flow evaporation) under a certain refrigerant saturation temperature. The battery heat generation is modeled as the constant heat flux. The main module is modeled as the conduction heat transfer. The real BTMS-DRC is constructed for experimentation with the test bench which is based on the dual-evaporators vapour compression refrigeration system. The simulated result is validated with the experimental results to ensure the correction of the modelling. The simulation also investigates the impact of the heat generation, convection heat transfer coefficient, refrigerant saturation temperature, and thermal conductivity on the module temperature and temperature uniformity. It is found that the simulated results agree well with the experimental results. The errors are around 2.9 - 7.2% throughout the specified working conditions. Overall, the proposed technique can be used as a design tool for further developing the BTMS-DRC.
Sterile Fecal Microbiota Transplantation Boosts Anti-Inflammatory T-Cell Response in Ulcerative Colitis Patients
Authors: Chechushkov Anton , Desyukevich Pavel , Yakovlev Timir , Allaf Lina Al , Shrainer Evgeniya , Morozov Vitalyi , Tikunova Nina
Published: December 2023

Ulcerative colitis is a chronic immune-mediated disease of unclear etiology, affecting people of different ages and significantly reducing the quality of life. Modern methods of therapy are mainly represented by anti-inflammatory drugs and are not aimed at a specific pathogenetic factor. In this study, we investigated the effect of transplantation of sterile stool filtrate from healthy donors on the induction of anti-inflammatory immune mechanisms. It was shown that performing such a procedure in patients with ulcerative colitis caused the appearance of T helper cells in the blood, which reacted to the content of sterile stool filtrates in an antigen-specific manner and produced IL10. At the same time, cells of the same patients before therapy in response to the addition of sterile stool filtrates were less reactive and predominantly produced IL4, indicating its pro-inflammatory skewing. The obtained data demonstrated the effect of an anti-inflammatory shift in the T-helper response after transplantation of sterile stool filtrate, which increased and persisted for at least three months after the procedure.
Environmental Sustainability Knowledge, Habits and Attitudes Among University and Non-university Population
Authors: Albedwawi Shaikha , Benhima Mohamed , Alsalami Aysha , Alshemeili Alreem , El-Tarabily Khaled A. , Al Raish Seham M.
Published: December 2023

The transition to sustainability requires major behavioural changes. This requires a complete rethinking of ecosystems and their interconnections, reassessing environmental issues, and a behavior change manifesting. Thus, understanding how sustainability Knowledge, Attitudes, and Practices (KAP) change is essential to sustainability as they shape human behaviour. The main aim of this study was to investigate the constructs of sustainability KAP among university and non-university populations. Data were collected using a structured questionnaire of items measured on a five-point Likert scale ranging from 1 (strongly disagree) to 5 (strongly agree). The questionnaire was distributed to diverse participants, ensuring a broad representation of views on sustainability. There are ten sociodemographic variables, 344 respondents in UAE where the female (28.5%) and male (13.7%), (58.1%) participants did not belong to the UAE university and their age fall into the 18-24 years old. The mean scores indicate a positive orientation towards sustainability, with Knowledge (M = 4.2272), Attitudes (M = 4.2650), and Practices (M = 3.8198) all above the midpoint and the UAE university students showed more knowledge, attitude and practices scores ‎than others population. Associations between KAP were significant at a significant level of p < 0.05. This indicates that the next generation will have more environmentally KAP.
Durable Textile Dyeing/Printing Using Natural Indigo Dyes &Amp; Leaves, and Mayan Inspired Blue Indigo Pigments.
Authors: Nemeshwaree Behary , Volle Nicolas
Published: December 2023

Indigo leaves form various plant species are sources of dyes/pigments, not fully exploited for making sustainable textiles. Blue indigo vat dye extracted from indigo leaves, yields high wash colorfastness but fades slowly with light, and is not easily used for direct printing. Indigo leaves can be used to produce textiles of various color shades, while light resistant Mayan-inspired hybrid pigments have not yet been used for textile coloring. Using blue indigo dyes from 3 plant species, with exhaustion dyeing, intense wash resistant blue colored textiles are produced, and in the case of Indigofera Persicaria tinctoria, textiles have antibacterial activity against S. Epidermis and E. Coli.100% Natural Mayan-inspired blue indigo pigment, made from Sepiolite clay and indigo dye, was used both in powdered and paste forms to perform pigment textile dyeing by pad cure process, and direct screen printing on textiles. A water-based bio-binder (40%) was used efficiently for both padding and printing. Bio-based Na Alginate thickener allowed color fast printing on both polyester and cotton fabrics, while bio-based glycerin allowed good print color fastness on polyester only, yielding prints with excellent color fastness to wash (5/5), to dry and wet rubbing (5/5) and light (7/7).
Safety and Efficacy of Tixagevimab and Cilgavimab for Prophylaxis of Covid-19 in a Cohort of Immunosuppressed Rheumatic Patients Treated with Rituximab: A Real-Life Experience
Authors: Peretti Silvia , Bonomi Francesco , Fiorentini Elisa , Bartoli Francesca , Lepri Gemma , Bellando-Randone Silvia , Guiducci Serena
Published: December 2023

(1) Background: Immunosuppressed patients, especially those receiving B-cells depleting therapies (BCDT), are at major risk to develop reduced vaccine seroconversion and contract severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) infection. The aim of our study is to assess safety and efficacy of the pre-exposure prophylactic combination of Tixagevimab and Cilgavimab (TGM-CGM) in a cohort of rheumatic patients diagnosed with Autoimmune Rheumatic Diseases; (2) Methods: We performed a prospective study using the clinical medical charts of 25 patients treated with Rituximab and received a single injection of TGM/CGM. The cohort was followed for 6 months from the injection and compared to a control group of 25 immunosuppressed patients who did not receive TGM-CGM. We assessed the incidence and the severity of Covid-19 in both groups, as well as early and late adverse events; (3) Results: Despite the small sample, we noticed a downward trend in the incidence and severity of symptomatic infection in the group treated with TGM/CGM. In the experimental cohort, one patient was completely asymptomatic, four patients were oligosymptomatic infection, and just one had mild-moderate infection versus 4 oligosymptomatic and 5 mild-moderate infection in the control group. We observed also a reduction in time to nasopharyngeal swab negativization. No adverse events were reported in our data. We collected the dosage of anti-Receptor-Binding Domain (RBD) SARS-CoV2 spike protein for 21 patients, revealing adequate seroconversion in 12 patients out of 21; (4) Conclusions: Even though the study was conducted during the Omicron wave, notably known to be less responsive to monoclonal antibodies, we proved that TGM-CGM could be a risk-free additional tool to prevent SARS-Cov2 infection in rheumatic immunosuppressed patients.
Prevalence of Cardiovascular Risks Factors in Cape Verde (Prevcardio.cv) – Santiago Island
Authors: Coelho Patricia , Mascarenhas Kelly , Rodrigues Julio , Rodrigues Francisco José Barbas
Published: December 2023

Cerebrovascular diseases represent a significant mortality rate worldwide and constitute a major public health and economic challenge. Many African countries such as Cape Verde are going through an epidemiological transition from communicable to non-communicable diseases, demographic and socioeconomic changes and facing an increase in cerebrocardiovascular diseases associated with increased cardiovascular risk. The present study aimed to identify the prevalence of risk factors that predispose to cardiovascular diseases in adult individuals residing on the island of Santiago. Cross-sectional population-based study with individuals over 18 years of age, of both sexes. Data collection took place in October and November 2021, in all municipalities on the island of Santiago. Individuals with Cape Verdean nationality who stayed abroad for more than one year and who arrived on the island of Santiago less than 30 days ago and individuals with any type of disability that could prevent them from participating in the study were excluded from the study. The sample size was calculated based on the projection of the population of the island of Santiago for the year 2021, according to the appropriate epidemiological model. Sociodemographic data were obtained through the application of a questionnaire containing age, sex, race, weight, height, risk factors (presence of diabetes, dyslipidemia, heredity, physical activity, smoking habits, history of cerebrovascular events, control of weight and blood pressure).
RESULTS: Study sample is predominantly female (54.8%), the age group with the largest number was 18-27 years (21%); 42.2% with BMI ≥ 25 kg/m2; 65.1% sedentary; Smoking 7.3% and alcoholism 14.4%; 6.5% with diagnosed heart disease; 19.9% with close family members with cardiac pathology; 2.7% had a stroke, 0.1% had a transient ischemic attack; 0.5% acute myocardial infarction; 23.5% had the periodic habit of assessing blood pressure; 32.6% hypertensive; 4.5% with diabetics.
CONCLUSIONS: The study showed that there is a high prevalence of cerebrovascular risk factors on Santiago Island, especially in females. Smoking and alcoholism showed a relationship with males. BMI, physical inactivity, diabetes and dyslipidemia were shown to be related to sex. Hypertension and Diabetes revealed a significant relationship with age group. Alcoholism, smoking, hypercholesterolemia and hypertriglyceridemia maintained a significant relationship with the municipalities.
Unveiling the Probiotic Potential of Streptococcus thermophilus MCC0200: Insights from In-vitro Studies corroborated with Genome Analysis
Authors: Kapse Neelam , Pisu Vaidehi , Dhakephalkar Tanisha , Margale Prajakta , Shetty Deepa , Wagh Shilpa , Dagar Sumit , Dhakephalkar Prashant
Published: December 2023

Streptococcus thermophilus is widely used as a starter culture in the dairy industry and has garnered attention as a beneficial bacterium owing to its health-promoting functionalities in humans. In this study, the probiotic potential of S. thermophilus MCC0200, isolated from a dairy product, was investigated through a combinatorial approach of in-vitro and in-silico studies. MCC0200 demonstrated the ability to survive harsh gastro-intestinal transit (GIT), adhere to intestinal mucosa and exert health-promoting traits in in-vitro studies. These findings were corroborated with in-silico evidence, wherein MCC0200 genome harboured genes associated with tolerance to GIT conditions, intestinal adhesion and colonization. Genome mapping also highlighted the ability of MCC0200 to produce compounds advantageous for host (folate, bacteriocins), to release enzymes that can reinforce the host ability (antioxidative enzymes), to metabolize food components that can be harmful to sensitive people (lactose). MCC0200 also demonstrated a positive effect on reducing cholesterol levels, proving to be a potential candidate for food and pharmaceutical applications. Absence of transmissible antibiotic resistance genes and virulence genes underscored the GRAS nature of MCC0200. This study explored the potential of Streptococcus thermophilus for its probable applications as a probiotic beyond the dairy industry.
NaCl Tolerance during Germination and Seedling Stages of Tomato (Solanum lycopersicum L.) Lines Native to Mexico
Authors: López-Méndez Ariadna Goreti , Rodríguez-Pérez Juan Enrique , Mascorro-Gallardo José Oscar , Sahagún-Castellanos Jaime , Lobato-Ortiz Ricardo
Published: December 2023

Tomato is considered moderately sensitive to salinity, which detracts from the quality and yield of its fruit; therefore, wild populations have been used as a genetic resource. The aim of this research was to identify lines derived from wild tomato populations with tolerance to salinity during the germination and seedling stages. During germination, 52 wild lines and 2 commercial hybrids were subjected to 150 mM and 0 mM NaCl and evaluated. The test was carried out for 20 days in a germination chamber with constant darkness, temperature of 25±2 °C and relative humidity of 80±4%. At the seedling stage, 22 wild tomato lines with the best performance in the germination test and 2 commercial hybrids were evaluated for 12 days in a floating raft system. Concentrations of 175 mM and 0 mM NaCl were used. Seventeen tolerant genotypes were identified at one of both developmental stages, while one genotype showed tolerance at both stages. These results indicate that there are different response mechanisms in each developmental stage. Native tomatoes play an important role in the identification of tolerant genotypes since they can be used as genetic resources for obtaining commercial genotypes with salt tolerance or as potential rootstocks.
Identification of New c-FLIP Inhibitors to Restore Apoptosis in TRAIL-Resistant Cancer Cells
Authors: Yaacoub Katherine , Pedeux Rémy , Lafite Pierre , Jarry Ulrich , ACI-Seche Samia , Bonnet Pascal , Daniellou Richard , Guillaudeux Thierry
Published: December 2023

The catalytically inactive caspase-8-homologous protein, c-FLIP, is a potent antiapoptotic protein highly expressed in various types of cancers. c-FLIP competes with caspase-8 for binding to the adaptor protein FADD (Fas-Associated Death Domain) following Death Receptors (DRs) activation by the ligands of the TNF-R family. As a consequence, the extrinsic apoptotic signaling pathway involving DRs is inhibited. Inhibition of c-FLIP activity in tumor cells might enhance DR-mediated apoptosis and overcome immune and anticancer drug resistance. Based on an in silico approach, the aim of this work was to identify, new small inhibitory molecules able to bind selectively to c-FLIP and block its anti-apoptotic activity. Using a homology 3D model of c-FLIP, an in silico screening of 1880 compounds from the NCI database (National Cancer Institute) was performed. Nine molecules were selected for in vitro assays, based on their binding affinity to c-FLIP and their high selectivity compared to caspase-8. These compounds selectively bind to the Death Effector Domain 2 (DED2) of c-FLIP. We have tested in vitro the inhibitory effect of these 9 molecules using the human lung cancer cell line H1703 overexpressing c-FLIP. Our results showed that 6 of these newly identified compounds efficiently prevent FADD/c-FLIP interactions in a molecular pull-down assay, as well as in a DISC Immunoprecipitation assay. Overexpression of c-FLIP in H1703 prevents TRAIL-mediated apoptosis, however, combination of TRAIL with these selected molecules signifi-cantly restored TRAIL-induced cell death by rescuing caspase cleavage and activation. Altogether, our findings indicate that new inhibitory chemical compounds efficiently prevent c-FLIP recruit-ment into the DISC complex, thus restoring caspase-8 dependent apoptotic cascade. These results pave the way to design new c-FLIP inhibitory compounds that may serve as anticancer agents in tumors overexpressing c-FLIP.
Organizational Behavior as a key factor to the success of Sustainable Policies and Actions: A research in the Greek Healthcare Sector
Authors: Sepetis Anastasios , Parlavantzas Ioannis , Goula Aspasia , Pierrakos Georgios , Rizos Fotios
Published: December 2023

This study examines the attitudes of healthcare organizations and employees in the Greek Healthcare System towards climate change and the influence of management in promoting environmentally friendly practices. A questionnaire was sent electronically to the 7 Health Districts of Greece and private sector healthcare workers associated with the researchers, while the sample consisted of service (administration, nursing, technical, and medical). The main findings indicate that public sector health organizations are lacking in motivating employees to adopt "green" actions during their work. Communication and information dissemination from administration to healthcare professionals were identified as significant challenges, resulting in participants being unsure of how to respond, in contrast to the private sector which showed slightly more positive results. Moreover, the study highlights a positive association between education level and voluntary green behaviors of employees. This research emphasizes the urgency to confront climate change in conjunction with the principles of Organizational Behavior within the Greek Healthcare System.
STUDY OF A DISTRICT HEATING SYSTEM WATER BOILER AS A OBJECT OF CONTROL
Authors: Snurnykov D. V. , Krasnikov Igor , Babichenko Anatoliy
Published: December 2023

The analysis of the operating conditions of a typical district heating system, in particular, a hot water gas boiler, was carried out. It is shown that the boiler, as the main control object, operates under conditions of constant change in external heat load, which, due to their random nature, leads to a number of uncertainties. The expediency of the mathematical description of uncertainties using the stochastic method as the most tested in practical conditions is substantiated. According to the results of the passive experiment on the hot water gas boiler KVG6.5-150 of the district heating system of one of the districts of Kharkov, an array of hourly experimental data was obtained, reflecting the main performance indicators of the hot water boiler. As a result of data processing by the least squares method, a mathematical model of the boiler was obtained in the form of a linear regression equation, reflecting the relationship between the temperature of the coolant at the boiler outlet and the ambient air temperature, the temperature of the coolant at the inlet to the boiler and with the flow rates of natural gas and coolant to the boiler. The obtained regression equation was verified by Student's t-test, which confirmed the significance of all coefficients of the regression model. The practical significance of the multiple regression equation was assessed using the coefficient of determination. The quality of the multiple regression equation as a whole was assessed using Fisher's F-test. Since parallel surveys were not conducted, instead of checking the adequacy, the quality of the approximation of the experimental points by the accepted regression equation was assessed, that is, it was checked whether this equation makes sense. Such a test was carried out by comparing the residual variance and the variance relative to the mean. The calculation results showed that the value of the coefficient of determination significantly exceeds the allowable value, and the actual value of the Fisher criterion significantly exceeds the table value. The obtained indicators led to the conclusion that the relationship between the variables in the regression model is significant, and the proposed stochastic method and the multiple linear regression equation can be used to make decisions in the process of synthesizing the technical structure of a computer-integrated control system for objects of a district heating system.
SIMULATION OF OSCILLATIONS OF WATER MOLECULES USING THE MODEL OF A TWO-FREQUENCY PENDULUM IN THE JAHN-TELLER POTENTIAL
Authors: Malafayev N. T. , Gaponova O. O.
Published: December 2023

The interaction potential for water molecules is considered, which corresponds to the presence of bends in hydrogen bonds in it. To explain the nature of this phenomenon, the theory of the Jahn-Teller effect (JTE) is applied. A model of the Jahn–Teller potential (JTP) was constructed, which has a minimum at a certain angle Θ. To simulate oscillations of water molecules in JTP using a pendulum, a correction of the angular potential U1 in the potential of directed forces (PDF) of intermolecular interaction is proposed using a wavelet type additive ΔU1 = c cos(mΘ)/exp(sΘ2). The parameters for the wavelet are selected based on the magnitude of the bending of hydrogen bonds in water (m=s=8, c=0…0.1). Modeling of rotational oscillations of molecules in JTP was carried out using the model of a two-frequency pendulum, which takes into account the ratio of the moments of inertia of the molecule (pendulum) along the axes k=3 and the PDF index n=8 in JTP Un =U1n. New types of pendulum oscillations in the JTP are determined in comparison with the original PDF (c=0) and their features. It is established that several types of oscillations are observed for this potential. These are new: sector, rotation of the pendulum in the potential trough – disordered or ordered, as well as types for PDF: two-frequency independent oscillations (IO) along the axes and ellipse-like oscillations (ELO) at one frequency. Oscillations in the JTP chute are observed for the main range of initial velocities inside an elliptical ring compressed along the Y axis, and only for the ELO, in an elliptical region elongated along the Y axis, as in the PDF. The methodology for calculating and analyzing the oscillation parameters of a two-frequency pendulum has been improved. The boundaries of oscillation types are determined for given parameters of the potential and a number of initial data. The difference between the patterns of oscillations is established for cases when the initial displacement of the pendulum is greater or less than the position of the minimum of the JTP. It is shown that the velocities at the boundary of the transition from the rotation of the pendulum in the potential trough to the IO correlate with the magnitude of the JTP maximum on the axis of the pendulum. The presence of stable transverse vibrations in the JTP for the case of protons of water molecules can apparently be considered as a new degree of freedom for vibrations of water molecules, which can lead to an explanation of the large contribution of rotational vibration modes to its heat capacity.