bluuebunny/crossref_metadata_2025 huggingface dataset:
Short Communication: The study of suweg (Amorphophallus paeoniifolius) and other undergrowth species in teak plantation forest of Temengeng, Blora, Indonesia
Authors: HIDAYAT SYAMSUL
Published: March 2019

Hidayat S. 2019. The study of suweg (Amorphophallus paeoniifolius) and other undergrowth species in teak plantation forest of Temengeng, Blora, Indonesia. Biodiversitas 20: 37-42. Suweg (Amorphophallus paeoniifolius (Dennst.) Nicolson) is one of the potential sources of food plants that are often found growing wild in farmers' gardens or fields. In the teak plantation forest of Cepu, precisely in Temengeng village, there are many suwegs growing among the old and big teak trees. To find out the potential and range of habitat where suweg grows, especially in teak plantation forest, ten observation plots were made using purposive sampling method, each measuring 10 x 10 m2. Overall, 17 adults and 30 young individuals of suweg were found. In addition to teak, 43 wild plant species were identified alongside suweg, from various habits, namely herb, liana, shrub, epiphyte, and tree. Herbs were the most common plants growing around suweg, especially from the families of Fabaceae, Compositae, and Zingiberaceae. The species that were almost always found together in the suweg plots were Tetrastigma lanceolarium (Roxb.) Planch. and Thespesia lampas (Cav.) Dalzell, but in quantity, Costus spiralis (Jacq.) Roscoe was more abundant than the other two species, although it was only found in four sampling plots. The results of this study also showed that there was no association between suweg and certain plants; this was supported by the low similarity index among plots.
Spatio-temporal distribution of microalgae producing chlorophyll and carotenoid pigments in Bali Strait, Indonesia
Authors: FAUZIAH ANNA , BENGEN DIETRIECH G , KAWAROE MUJIZAT , EFFENDI HEFNI , KRISANTI MAJARIANA
Published: March 2019

Fauziah A, Bengen DG, Kawaroe M, Effendi H, Krisanti M. 2019. Spatio-temporal distribution of microalgae producing chlorophyll and carotenoid pigments in Bali Strait, Indonesia. Biodiversitas 20: 61-67. The Bali Strait waters become a place for various development activities that connect Java Island and Bali Island so that it is thought to have an impact in the life of microalgae that play an important role in the wealth of their water resources. This study aims to explore the spatiotemporal distribution of marine microalgae, which has the potential to produce chlorophyll and carotenoid pigments, as well as their relationship with the environmental characteristics of the Bali Strait waters. The research was conducted at the 5 stations in the morning, midday and afternoon. The data obtained were analyzed using Principal component analysis (PCA) and Correspondence analysis (CA). The results showed that chlorophyll-a, chlorophyll-b and carotenoid were mostly contained by the species Chaetoceros gracilis, Tripos lunula at station 5 (Pang-pang Bay) in the morning, midday and afternoon, and contained by the species Fragilariopsis cylindrus, Thalassiothrix fravenfeldii at station 3 (Ketapang-Gilimanuk ferriage) in the midday and afternoon. Spatially, salinity, ammonia, nitrate, and chlorophyll-a have significantly different values between stations, while temporally the content of chlorophyll-b and carotenoid are influenced by time (in the morning, midday and afternoon).
Local monitoring of flowering and fruiting of Jernang, Daemonorops species in Sumatra, Indonesia
Authors: SCHMIDT LARS , RUSTIAMI HIMMAH , THEILADE IDA
Published: March 2019

Schmidt L, Rustiami H, Theilade I. 2019. Local monitoring of flowering and fruiting of Jernang, Daemonorops species in Sumatra, Indonesia. Biodiversitas 20: 118-125. Phenology and fruit production recordings of Jernang (Daemonorops spp.) were conducted in southern Sumatra over two one-year periods. Flowering and fruiting of 64 individual clumps with 570 stems > 2 m and 43 clumps with 478 stems > 2 m respectively were recorded on a monthly basis. Results showed that flowering and fruiting of Jernang took place throughout the year with a peak in April and May and at low level in September to March. Duration of male inflorescences was 2-3 months. Fruits were usually harvested for resin 2-3 months after flowering. Sex ratio of clusters was about 1:1. Flowers and fruits were produced on vigorously growing stems that had reached canopy light exposure. Clusters consisted of from 2 to 36 stems. In some clusters, all stems bore reproductive organs; in others, only few stems had flowers or fruits. Female clusters had significantly more stems than male clusters, but male clusters produced more inflorescences than did females. About 80% of the clusters during the first year’s survey and 50% the second year produced flowers. The most vigorous male clusters produced more than 80 inflorescences with up to 15 on a single stem within a year. The highest producing female clusters produced 12 inflorescences in a year with up to five inflorescences and four infructescences per stem in a year. The data was primarily collected by trained local monitors. They knew the species well and were used to spot flowers and fruits. However, since the local monitors were also all Jernang collectors, they usually picked fruits before maturity, and the full development into mature fruits was thus interrupted.
Growth evaluation of native tree species planted on post coal mining reclamation site in East Kalimantan, Indonesia
Authors: AYU LESTARI DEWI , PUTRI FIQA ABBAN , FAUZIAH FAUZIAH , BUDIHARTA SUGENG
Published: March 2019

Lestari DA, Fiqa AP, Fauziah, Budiharta S. 2019. Growth evaluation of native tree species planted on post coal mining reclamation site in East Kalimantan, Indonesia. Biodiversitas 20: 134-143. Mining activities affect environmental qualities including the loss of vegetation cover and the damages of physical, chemical and biological properties of soil. As such, regulations enacted by the Indonesian Government state a legal obligation for mining companies to carry out reclamation on post coal mining site. Reclamation is an activity carried out to organize, restore and improve of environmental quality after mining operations to enhance the highly disturbed ecosystem of mined land into ecologically usable state. Yet, there is little body of knowledge on how to monitor the effectiveness of reclamation in improving environmental quality of ex-mining land. Aims of this study are to evaluate of the growth of native tree species planted on various types of reclamation sites in a mining concession in East Kalimantan, and to analyze the most influencing factor of their growth. Growth parameters measured in this research were plant height, stem diameter and branch-free stem height of the planted species. Micro-climatic factors and diversity of understorey plants were also measured as environmental parameters. PCA (Principal Component Analysis) analysis was conducted using PAST 4.0. statistical program. The results shows that reclamation area of post coal mining in the study site which is most suitable for local plant species, especially Shorea balangeran, has a sloping terrain. PCA shows that factor having maximum influence on growth of planted species on the reclamation site is pH of soil. The higher is the soil pH (i.e. less acid), the better is the species growth because the soil conditions in the post-coal mining area tend to be acidic. This research suggests that in post coal mining reclamation using native trees two key factors to enhance growth performance of planted species are sloping terrain so as water is not inundated and soil pH so as it is not too acid.
Local biodiversity conservation in Sigi, Central Sulawesi, Indonesia: Analysis of the effect of elevation, land accessibility, and farmers’ income and perception on vegetation diversity in agroforestry systems
Authors: DWI LESTARI NINA , SUPRAYOGO DIDIK , RACHMANSYAH ARIEF
Published: March 2019

Lestari ND, Suprayogo D, Rachmansyah A. 2018. Local biodiversity conservation in Sigi, Central Sulawesi, Indonesia: Analysis of the effect of elevation, land accessibility, and farmers’ income and perception on vegetation diversity in agroforestry systems. Biodiversitas 19: 283-291. The objectives of the research were to evaluate the level of landscape vegetation diversity in agroforestry systems and to determine the effect of elevation, accessibility, and farmer's income and perception on vegetation diversity in agroforestry systems. The research was conducted in Sigi District, Central Sulawesi, Indonesia. The method used was the Multidisciplinary Landscape Assessment approach. The research sites were located in 3 districts and 6 representative villages i.e. Padende and Sibedi in the lower landscape (23-75 m asl.), Balane and Doda in the middle landscape (148-504 m asl.), Dombu and Ongulero in the upper landscape (1163-1404 m asl.), with 5 replications in each village. The total area of the six villages was 3772.13 ha that consisted of 1217.13 ha of agroforestry systems (32.3%). The research covered a population of 392 households who had landed with agroforestry systems. A total of 30 agroforestry farmers and their land (minimum 0.25 ha, homogeneous land system) were selected as samples. Research activities like observation, survey, and analysis were divided into two aspects, namely biophysical and socioeconomic aspects. The results revealed that at landscape level agroforestry systems had relatively high plant species diversity (H'= 3.1) and needed to be optimized to enhance its role as buffering zone for biodiversity conservation and for supporting sustainable livelihood. Efforts to improve vegetation diversity must be adjusted to habitat conditions (elevation and soil health), where vegetation can grow and produce well. The regional development such as accessibility improvement is still needed and it will not threaten the vegetation diversity as long as there is a harmony between human activities and the environment. An increase in the level of income with a positive perception of farmers on the benefits of vegetation diversity in the future will have a main role in vegetation diversity conservation as fundamental asset in sustainable development.
Using micro-morphological fruit characters in resolving some of ambiguities in Iranian Acer L. (Sapindaceae) species
Authors: ASADI FATEMEH , SHARIFNIA FARIBA , SALIMPOUR FAHIMEH , MAJD AHMAD , MAJD AHMAD
Published: March 2019

Asadi F, Sharifnia F, Salimpour F, Majd A. 2019. Using micro-morphological fruit characters in resolving some of ambiguities in Iranian Acer L. (Sapindaceae) species. Biodiversitas 20: 297-304. Acer L. belongs to the Sapindaceae family consists of 126 species which are distributed in the temperate regions of the northern hemisphere. There are 12 taxa (including Acer monspessulanum with four subspecies and Acer velutinum with two varieties) in Iran. In this study 21 fruit characteristics are partially investigated using Scanning Electron Microscope (SEM) and Stereomicroscope. According to the obtained results, four types of faveolate, folded, wrinkled and pavement wing surface ornamentations as well as four types of faveolate, folded, verrucous, and wrinkled achene surface ornamentations were observed. Hair of wing margin, achene hair, wing ornamentation, inside angle of wing, and outside angle of wing have taxonomic value. According to fruit morphology, separation of 4 subspecies of A. monspessulanum can be verified. Also varieties of A. velutinum var. velutinum, and A. velutinum var. glabrescens were totally separated due to differences in characteristics such as wing ornamentation, achene ornamentation, hair density of achene, wing length, wing width, the ratio of length to width of wing, total fruit length, total fruit width, the ratio of length to width of fruit, inside angle of wing, outside angle of wing. The results showed that micro-morphological fruit characters of Acer genus are valuable in terms of taxonomy and classification of subspecies level.
Short Communication: Early stability test of mutant candidates of Bangka local cassava, Indonesia
Authors: LESTARI TRI , DYAH MUSTIKARINI ERIES , APRIYADI RION , ANWAR SAIPUL
Published: March 2019

Lestari T, Mustikarini ED, Apriyadi R, Anwar S. 2019. Early stability test of mutant candidates of Bangka local cassava, Indonesia. Biodiversitas 20: 337-342. Cassava production in Indonesia has decreased due to decreased harvest area. Increasing production can be done by improving genetic diversity through gamma-ray irradiation to create high production mutant. The purpose of this study was to determine the optimum irradiation doses in relation to improve production of local cassava from Bangka Island, as well as morphological changes resulting from the irradiation. The research was conducted at the Experimental Garden, the University of Bangka Belitung, Indonesia in September 2017 until April 2018. This experiment was using Split Plot design, with the main plot consisted of 2 levels and subplot have 3 treatment levels, and 4 replications, in total there were 24 experimental units. The results showed that gamma-ray irradiation had very significant effect on plant height, number of leaves, young leaf color, stem color, and number of lobes. Gamma-ray irradiation on 3 Bulan accession showed an improved tuber production, whereas in Malang varieties gamma-ray irradiation decreased tuber production. Gamma-ray irradiation dose of 30 Gray was able to improve the number of tubers, tuber weight and starch content on 3 Bulan cassava accession.
The diversity of Smilax (Smilacaceae) in Besiq-Bermai and Bontang Forests, East Kalimantan, Indonesia
Authors: SOFIAH SITI , DWI SULISTYANINGSIH LULUT
Published: March 2019

Abstract. Sofiah S, Sulistyaningsih LD. 2019. The diversity of Smilax (Smilacaceae) in Besiq-Bermai and Bontang Forests, East Kalimantan, Indonesia. Biodiversitas 20: 379-387. The genus Smilax has taxonomic complexity problems and spacious distribution. Taxonomic study to reveal the diversity of Smilax species had been done in some regions, such as America, China, Japan, Thailand, and Indonesia. However, there is lack of information of Smilax species diversity in Kalimantan especially in East Kalimantan which lies in Sundaland biogeographic. This study was carried out to explore and record the diversity of Smilax species including the ecological and environmental data in Besiq-Bermai and Bontang forests in East Kalimantan, Indonesia. This research conducted on February and August in 2012 and July-August 2015 using exploration methods. Purposive random sampling was used to do the botanical sampling. The principal component analysis (PCA) was performed to determine the relationships between environmental components and Smilax species occurrences. There were five species of Smilax which were housed in those forests in East Kalimantan, namely, Smilax leucophylla Blume, Smilax gigantea Merr., Smilax odoratissima Blume, Smilax zeylanica L., and Smilax modesta A.DC. Smilax leucophylla and Smilax zeylanica are the most widely used by the local people for medicine. The taxonomic description, distribution, use, and vernacular name were given. The environmental factors that contribute significantly to Smilax's growing environment are temperature and light intensity.
Genetic diversity of Ralstonia solanacearum, A phytopathogenic bacterium infecting horticultural plants in Java, Indonesia
Authors: MERRY HEMELDA NIARSI , SAFITRI RAMADHANI , SUHANDONO SONY
Published: March 2019

Hemelda NM, Safitri R, Suhandono S. 2019. Genetic diversity of Ralstonia solanacearum, A phytopathogenic bacterium infecting horticultural plants in Java, Indonesia. Biodiversitas 20: 364-372. Ralstonia solanacearum is a phytopathogenic bacterium causing bacterial wilt disease which has been reported to infect many important plants in Indonesia. However, less study has been done on its genetic diversity in Indonesia. This study aimed to investigate the genetic diversity of R. solanacearum virulent strains isolated from Java Island. BOX-PCR fingerprint was carried out to investigate the genetic pattern of R. solanacearum isolates, while phylogenetic analysis of mutS gene was performed to determine the genetic diversity of R. solanacearum isolates. A total of 21 isolates was obtained from four crop species: twenty isolates identified as phylotype I while one isolate identified as phylotype II. Based on BOX-PCR, most of the isolates clustered according to their original provinces, indicating site-dependent distribution pattern. However, BOX-PCR also detected site-contaminations indicated by the similar genetic patterns found in two provinces. Phylogenetic analysis of mutS gene discovered that most of the phylotype I isolate showed 100% similarity to each other, while phylotype II isolate belonged to phylotype IIB and showed 100% similarity to IPO1609 strain. Pathogenicity and biovar test were confirmed that the phylotype IIB isolate belonged to the R3bv2 strain which was adapted in low temperature. This study provided the first description about genetic diversity patterns of R. solanacearum strains in Java Island and revealed new challenges related to how to prevent contamination of R. solanacearum from one province to another, as well as the phylotype IIB strain infection in a highland area in Indonesia.
Short Communication: Positional behavior of captive Bornean Orangutan (Pongo pygmaeus) at Bukit Merah Orangutan Island, Perak, Malaysia
Authors: NORSYUHADA KAMALUDDIN SITI , DHARMALINGAM SABAPATHY , MUNIR MD-ZAIN BADRUL
Published: March 2019

Kamaluddin SN, Dharmalingam S, Md-Zain BM. 2019. Short Communication: Positional behavior of captive Bornean Orangutan (Pongo pygmaeus) at Bukit Merah Orangutan Island, Perak, Malaysia. Biodiversitas 20: 425-429. Bukit Merah Orangutan Island (BMOUI) is the only semi-captive orangutan conservation center in the Malay Peninsula. BMOUI promotes orangutan conservation through ex-situ efforts comprising rehabilitation, education, awareness, ecotourism, and research studies. This research aimed to determine the qualitative behavior aspects, especially focusing on the orangutan positional behavior on BMOUI. A focal instantaneous sampling method was carried out for 252 hours from December 2015 to November 2016. The qualitative results showed that the positional behaviors of a Bornean orangutan at BMOUI were divided into nine main categories, as follows: sitting, lying, clinging, forelimb-hindlimb suspension, standing, walking, running, brachiation, and multipositional modes. The most favorable position shown by a captive Bornean orangutan on BMOUI were dominated by sitting and lying postures. The variety of positional modes displayed in a semi-captive condition still maintained Bornean orangutan as the great ape originating from the tropical inhabitants of wooded environments.
Importance of the Congonhas River for the conservation of the fish fauna of the Upper Paraná basin, Brazil
Authors: GARCIA DIEGO AZEVEDO ZOCCAL , VIDOTTO-MAGNONI ANA PAULA , COSTA ALEXANDRO DERLY AUGUSTO , CASIMIRO ARMANDO CÉSAR RODRIGUES , JARDULI LUCAS RIBEIRO , FERRAZ JOÃO DANIEL , DE ALMEIDA FERNANDA SIMÕES , ORSI MÁRIO LUÍS
Published: March 2019

Abstract. Garcia DAZ, Vidotto-Magnoni AP, Costa ADA, Casimiro ACR, Jarduli LR, Ferraz JD, De Almeida FS, Orsi ML. 2019. Importance of the Congonhas River for the conservation of the fish fauna of the Upper Paraná basin, Brazil. Biodiversitas 20: 474-481. The Upper Paraná River basin is among the most fragmented watersheds in the world. The Congonhas River belongs to this drainage system, which has its mouth in the Capivara Reservoir and can be considered the largest tributary free-from-dam, becoming important for the maintenance of fish species richness. In order to know more about the species present in this tributary, we aimed to provide an inventory of fish fauna of the Congonhas River and reproductive strategies to determine whether the river is used by migratory species and whether there is a longitudinal variation in the fish assembly. These findings will provide subsidies to contribute to the implementation of policies to protect biodiversity. Samplings occurred in the upper, middle and lower stretches of the Congonhas River during four periods between 1991 and 2011. Fishes were captured using gill nets from 2 to 14 cm mesh sizes between opposite knots and cast nets, identified and categorized according to their reproductive strategies. A total of 4,640 individuals belonging to 63 species, of which 79.4% consisted of Characiformes and Siluriformes were captured. Furthermore, 14% of fish were long-distance migratory species (e.g., Piaractus mesopotamicus, Megaleporinus obtusidens, Prochilodus lineatus, Salminus brasiliensis, Pinirampus pirinampu, and Pseudoplatystoma corruscans). In addition, there was an increase in the fish species richness from upstream to downstream. Management actions such as prohibition of fishing, rehabilitation of riparian vegetation, and protection of the basin against dam construction will reduce anthropogenic impacts on the ecosystem. The Congonhas River highlights the importance of preserving lotic environmental for the conservation of migratory and non-migratory Neotropical fish diversity into a heavily modified watershed.
The diversity and physiological activities of weeds in land cultivated with various corn cultivars and fertilized with various nitrogen doses
Authors: RAHAYU MUJI , YUDONO PRAPTO , INDRADEWA DIDIK , HANUDIN EKO
Published: March 2019

Abstract. Rahayu M, Yudono P, Indradewa D, Hanudin E. 2019. The diversity and physiological activities of weeds in land cultivated with various corn cultivars and fertilized with various nitrogen doses. Biodiversitas 20: 622-628. Both weeds and corn have nutrient needs. Nitrogen is one of the essential elements required by weeds and corn. Each corn cultivar and weed also has different ability to absorb nitrogen. This study aimed to determine the effect of corn cultivar and nitrogen dose on the diversity and physiological activities of weeds. The research was conducted in Banguntapan, Bantul, Yogyakarta from December 2016 to May 2017. The study used a completely randomized block design with 2 factors with three replications. The first factor was corn cultivar, consisting of four cultivars (Bisi 18, NK 33, DK 95 and Sukmaraga) and the second factor was the nitrogen dose, consisting of three levels (25 kg N ha-1, 150 kg N ha-1 and 275 kg N ha-1). The data were analyzed using 5% variance analysis and continued with Duncan test at 5% level. The results showed that the most commonly found weeds were broadleaves, but the most dominant weed was a grass, namely Dactyloctenium aegyptium, except Sukmaraga with nitrogen doses of 25 kg ha-1 and 150 kg ha-1 in 4 weeks after planting (WAP). In 8 WAP, weeds that grew in land planted with all combinations of cultivars and nitrogen doses were Cyperus rotundus, Dactyloctenium aegyptium, Digitaria ciliaris, Eleusine indica, Eragrostis tenella, Amaranthus spinosus, and Richardia scabra. There were no significant differences on the nitrate reductase activity, chlorophyll content, leaf area index, and dry weight among cultivars. The treatment of 25 kg N ha-1 resulted in the lowest leaf area index and total chlorophyll content, but the highest nitrate reductase activity.
Delineation of the selected Cucumis L. species and accessions using leaf architecture characters
Authors: LAILANI A. MASUNGSONG LAILANI A , BELARMINO MARILYN M , BUOT JR INOCENCIO E
Published: March 2019

Abstract. Masungsong LA, Belarmino MM, Buot IEJr. 2019. Delineation of the selected Cucumis L. species and accessions using leaf architecture characters. Biodiversitas 20: 629-635. Regardless of the several attempts of the early and recent studies to separate the wild species of Cucumis from the cultivated ones, there is still taxonomic confusion brought about by the similarities in morphology of the genus. In a gene bank with so many species and accessions of Cucumis stored, it is appropriate to delineate these numerous accessions to save time and resources as well. This study aims to delineate fifty selected Cucumis accessions based on leaf architecture. Using Unweighted Pair Group Method using Averages (UPGMA) and Euclidean distance coefficient, a cluster analysis for the fifty Cucumis accessions was done. A dendogram with cophenetic coefficient of 0.9606 supported the clustering of the Cucumis species and accessions. At Eucledian distance of 1.5 two major clusters were formed on the basis of secondary vein spacing. Cucumis melo accessions separated from all the remaining accessions of C. myriocarpus, C. metuliferus, C. anguria and C. anguria var longaculeatus for having an increasing towards the base secondary vein spacing while the rest have irregular pattern of secondary vein spacing. Further sub-clustering of the remaining accessions comprising four species were delineated on the basis of tertiary vein (C. myriocarpus), tertiary vein angle to primary (C. metuliferus), and blade class (C. anguria and C. anguria var longaculeatus). Laminar shape delineated C. myriocarpus accessions from each other, apex angle for C. metuliferus accessions, and primary vein size for C. melo accessions. Results implied that leaf architecture is a good tool to classify the numerous accessions of Cucumis.
Ecology of Zanthoxylum acanthopodium: Specific leaf area and habitat characteristics
Authors: JUNAEDI DECKY INDRAWAN
Published: March 2019

Abstract. Junaedi DI, Nurlaeni Y. 2019. Ecology of Zanthoxylum acanthopodium: Specific leaf area and habitat characteristics. Biodiversitas 20: 732-737.  Andaliman (Zanthoxylum acanthopodium) is an important species in North Sumatra, Indonesia either from conservation, economy, or socio-cultural point of views. Z. acanthopodium is known as plant species difficult to cultivate and its natural distribution in Indonesia is restricted to Aceh and North Sumatra, Indonesia. This study aims to identify the modest characteristics of Z. acanthopodium’s habitat in natural and cultivated areas and to assess the importance of light in these two habitat types in North Sumatra. We surveyed four districts in North Sumatra and used specific leaf area (SLA) as proxy for the importance of light in Z. acanthopodium habitat. We found that the species grow naturally in high slope areas and not in a plain area. Similarly, in cultivated areas, this species grows well in sloped area with minimum inundated surface run-off water. Z. acanthopodium with higher SLA values occurs in natural habitat and high slope areas while those with smaller SLA are found in cultivated areas and low slope although the difference is statistically not significant. This study indicates the importance of light and surface run-off for suitable habitat of Z. acanthopodium. Further studies are needed to examine the quality, intensity, and frequency of light to support Z. acanthopodium growth; and to examine the role of landscape inclination and their position relative to sun exposure (i.e. aspect) for its survival and growth rate.
Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia
Authors: HIDAYATURRAHMAH HIDAYATURRAHMAH , MABRUR MABRUR , SANTOSO HERI BUDI , SASMITA RANI , RAHMY UMMY SHALIHA AULIA , BADRUZSAUFARI BADRUZSAUFARI
Published: March 2019

Abstract. Hidayaturrahmah, Mabrur, Santoso HB, Sasmita R, Rahmy USA, Badruzsaufari. 2019. Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia. Biodiversitas 20: 745-753. Giant mudskipper (Periphthalmodon schlosseri, Pallas 1770) is one of important biological diversity that potential as bio-indicator in environment assessment which is heavy metal contamination. These fish are susceptible to ambient environment, that effect to their physiological mechanism including protein synthesis and expression. This research aims to identify protein profiles of giant mudskipper muscle and review its potential application as a biomarker for heavy metal contamination in Barito Estuary of South Kalimantan. Total proteins were isolated from fish muscle using Tris EDTA buffer and then precipitated using Ammonium sulfate salt. The protein concentration was measured using Bradford assay and then separated based on molecular weight using the SDS-PAGE method. The result showed muscle proteins of giant mudskipper could be precipitated on optimum condition at Ammonium sulfate saturation 60-70% which protein concentration 5.106 mg.mL-1. The protein separated into 20 bands for raw and precipitated proteins which molecular weight ranging from 33 into 184 kDa. Further study is needed for protein identification based on amino acids sequence of protein bands for biomarker discovery and validation.
Population and distribution of Sunda porcupine (Hystrix javanica F. Cuvier, 1823) in designated area of Cisokan Hydropower, West Java, Indonesia
Authors: MUSTIKASARI IRINA A , WITHANINGSIH SUSANTI , MEGANTARA ERRI N , HUSODO TEGUH , PARIKESIT PARIKESIT
Published: March 2019

Abstract. Mustikasari IA, Withaningsih S, Megantara EN, Husodo T, Parikesit. 2019. Population and distribution of Sunda porcupine (Hystrix javanica F. Cuvier, 1823) at designated area of Cisokan Hydropower, West Java, Indonesia. Biodiversitas 20:  762-769. One of the endemic species of Indonesia is the Sunda porcupine (Hystrix javanica). Sunda porcupine is a big rodent which has unique defense mechanism behavior using its quills. This research was conducted in the designated area of Cisokan Hydropower (PLTA Cisokan), in West Java, Indonesia. The method in this study is qualitative to determine the total population and distribution. Data collection techniques were carried out by sign surveys (burrow, footprints, quills, food sites, and feces), camera traps, and semi-structured interviews. The result shows the number of porcupine populations in the designated area of Cisokan Hydropower is 6-29 individuals. Porcupine population is distributed in five regions, namely the first region consisting of Pasir Gagak, Cigintung, Batu Sahulu and Batu Nunggul; the second region is Batu Tumpeng and Batu Wulung; the third region is Curug Japarana; the fourth region consists of Sarongge and Cadas Gantung; and the fifth region is Pongpok. Based on the type of land cover, the porcupine population is commonly found in the types of shrub/huma /ladang, talun, and natural forests.
Genetic variations of strawberry cultivars of Fragaria x ananassa and Fragaria vesca based on RAPD
Authors: ARISTYA GANIES RIZA , KASIAMDARI RINA SRI , SETYONINGRUM RACHMI , LARASATI BENING
Published: March 2019

Abstract. Aristya GR, Kasiamdari RS, Setyoningrum R, Larasati B. 2019. Genetic variations of strawberry cultivars of Fragaria x ananassa and Fragaria vesca based on RAPD. Biodiversitas 20: 770-775. In Indonesia, the increasing market demand for strawberries (Fragaria spp.) is not comparable to increased strawberry productivity. One of the efforts made to increase strawberry productivity with superior quality is plant breeding. The purpose of this research was to determine the genetic variation, lineage, and similarity index in some strawberry cultivars using molecular markers of Random Amplified Polymorphic DNA (RAPD). Eleven strawberry cultivar samples were taken from Indonesian Citrus and Subtropical Fruits Research Institute (Balitjestro), Batu City, East Java, Indonesia and Strawberry Agritourism in Banyuroto Village, Magelang District, Central Java, Indonesia. DNA isolation using modified CTAB buffer method. DNA amplification using PCR-RAPD method with 5 primers, namely UBC-516, UBC-594, OPA 10, OPA 16, and OPG 11. Strawberry lineage dendrogram construction was analyzed with clustering of Unweight Pair-Group Using Arithmetic Average (UPGMA) software Multi-Variate Statistical Average (MVSP). The research results showed that the 5 RAPD primers used in 11 strawberry cultivars produced 30 polymorphic DNA bands and 20 monomorphic DNA bands so it can be concluded that the genetic variation among 11 strawberry cultivars can be detected using RAPD molecular markers. The lineage of 11 strawberry cultivars that have the highest similarity index is found in Earlibrite and Rosalinda II cultivars of 98.85%.
Exploration culturable bacterial symbionts of sponges from Ternate Islands, Indonesia
Authors: TRIANTO AGUS , RADJASA OCKY KARNA , SABDONO AGUS , MUCHLISSIN SAKTI IMAM , AFRIYANTO RACHMAT , SULISTIOWATI SULISTIOWATI , RADJASA SEPTHY KUSUMA , CREWS PHILLIP , MCCAULEY ERIN
Published: March 2019

Abstract. Trianto A, Radjasa OK, Sabdono A, Muchlissin SI, Afriyanto R, Sulistiowati, Radjasa SK, Crews P, Mccauley E. 2019. Exploration culturable bacterial symbionts of sponges from Ternate Islands, Indonesia. Biodiversitas 20: 776-782. Multidrug resistance, (MDR) bacteria seriously threaten human health which causes a more difficult and extends the treatment period, and increases the risk of death. Sponges-associated bacteria, a well known for their rich in chemical classes and bioactivities, are the prolific producers of bioactive compounds. In order to search new sources of antibacterial compounds, we collected a total of 55 sponges from Ternate Island that provided 324 bacterial isolates. The data showed that sponges-isolates ratio in the anthropogenic area was relatively higher than the other collection sites. The isolates were screened for the antibacterial activity against Klebsiella pneumoniae-RSDK, K. pneumoniae-UI, Pseudomonas aeruginosa A-UI, P. aeruginosa B-UI, Staphylococcus aureus-UI, MRSA-UI, MRPA-UI, Bacillus subtilis-RSDK, B. subtilis-UI, Salmonella typhi-UI, and MDR E. coli. The isolates were able to inhibit 0-7 the pathogenic bacteria on 24 h and 48 h. The most active bacteria were identified as B. clausii, V. chiguensis, B. tropicus, P. marcusii, B. tropicus, V. parahaemolyticus, B. paramycoides, and V. dokdonensis. In conclusion, the sponges in the anthropogenic affected area have higher bacterial symbionts than that of in the pristine area. In this study, the results of isolation of the symbiont bacteria from sponge samples were obtained and morphologically observed for these bacterial isolates. The number of bacteria that were isolated were 133 bacterial isolates and ten isolates including producing active secondary metabolites
Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique
Authors: SUTARNO SUTARNO , ZAHRAH SYARIFA , ASTIRIN OKID PARAMA , HERAWATI ELISA , SETYAWAN AHMAD DWI
Published: March 2019

Abstract. Sutarno, Zahrah S, Astirin OP, Herawati E, Setyawan AD. 2019. Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique. Biodiversitas 20: 783-788. Genetic diversity is the basis of livestock breeding because it can be used as an initial improvement in livestock quality through artificial selection. This study aims to determine polymorphism in ND-5 fragment of mitochondrial DNA in Ongole Grade, Aceh, and Sumbawa cattle and their genetic diversity. The total DNA from the blood of the local cattle was extracted using the Wizard genomic DNA purification system from Promega and amplified using the PCR technique. The PCR product was then digested with HindIII enzyme using the RFLP technique to detect polymorphism. The genetic diversity of the Ongole Grade, Aceh, and Sumbawa cattle was analyzed using the formula from Nei and its genetic relationship was evaluated with the 2.02i NTSYSpc version program. Our findings showed there were polymorphisms in the ND-5 fragment of mitochondrial DNA. Digestion with HindIII restriction enzyme produces two types of haplotypes. Haplotype B is a 453 bp-sized DNA fragment that is not truncated by the HindIII enzyme, and haplotype A is a DNA fragment cut by HindIII enzyme into two with fragments of 336 bp and 117 bp. Polymorphism was found in Ongole Grade cattle, but not in Sumbawa and Aceh cattle. Haplotypes diversity in ND-5 fragments of mitochondrial DNA of Ongole Grade was 0.6250 while Sumbawa and Aceh cattle displayed no diversity of haplotypes. The genetic relationship shows that Sumbawa cattle belonged to the same cluster with Ongole Grade but separated from Aceh cattle.
An exploration of gender equity in household: A case from a peatland-based community in Riau, Indonesia
Authors: HERAWATI TUTI , ROHADI DEDE , RAHMAT MAMAT , WINARNO BONDAN
Published: March 2019

Abstract. Herawati T, Rohadi D, Rahmat M, Winarno B. 2019. An exploration of gender equity in household: A case from a peatland-based community in Riau, Indonesia. Biodiversitas 20: 853-861. Gender roles in peatland-based communities’ livelihoods are an important factor in livelihood vulnerability due to challenging characteristics for conducting agriculture in this area. This article reports on a study of gender roles in Riau Province in 7 villages in 3 districts, aiming to identify significant attributes that determining gender equity at the household level. The data was collected through structured interviews in 221 households, in-depth interviews and Focus Group Discussions in every village. The research indicated that gender roles in agricultural activities are significantly dominated by men, while women play a more significant role in domestic activities. Both men and women contribute equally in the social life of the community; women’s participation and group membership is equal to men’s. Poor families tend to have higher gender equity in agricultural activities than rich households. The role of women in wealthier households is not in their physical contribution on the land but is mostly transformed into a decision making role. This indicates that women have a significant role in family livelihoods for both poor and rich families, but in different forms.
Some structural characteristics of mixed natural forest stand at a 10-year period in the Hyrcanian Region: Case study of Shourab Forest, Mazandaran Province, Iran
Authors: AMIRI MOJTABA
Published: March 2019

Abstract. Amiri M. 2019. Some structural characteristics of mixed natural forest stand at a 10-year period in the Hyrcanian Region: Case study of Shourab Forest, Mazandaran Province, Iran. . Biodiversitas 20: 950-955. This research was carried out in Shourab Forest in the Hyrcanian region, Northern Iran over a 10-year period (1996-2005) and data were analyzed based on systematic random sampling with permanent sample plots; 0.1 ha. The results revealed that n.ha-1 and v.ha-1 in study area increased from 126.3 and 245.2 in 1966 to 202.8 and 313.1 in 2006, respectively. The analysis of tree frequency in diameter classes at the first and the end of the period indicated un-even aged regular stands. The highest increase of number of trees was observed in 15, 20 and 25cm diameter classes, While the maximum change in volume per hectare, is related to the diameter classes 30, 65, 70 and 100cm. The regeneration values in most of the vegetation stages (except; 0-2.5cm) increased after a 10 years harvesting period. Development stages of this regeneration were seedling (53.4-53.3%), sapling (37.3-33.5%), thicket (6.8-10.4%) and small pole (2.5-2.8%), in 1996 and 2006, respectively. These conditions show that the selection method has been successful in our study area. The change in the utilization method of shelter-wood system to selection cutting method had been a positive impact on the quantitative forest condition after a 10-year period. In general, it can conclude that structural characteristics of mixed beech-hornbeam stands such as live trees dimension including tree height and diameter, number and volume of trees per hectare are different during the time. And eventually, the manipulation in the stands should be well timed and planned based on quantified structural properties of stands.
Short Communication: Agricultural biodiversity and economic productivity of the yards in Arguni Bawah, Kaimana District, West Papua Province, Indonesia
Authors: ANTOH ALFRED ALFONSO , ARIFIN NURHAYATI HS , CHOZIN M A , ARIFIN HADI SUSILO
Published: March 2019

Abstract. Antoh AA, Arifin NHS, Chozin MA, Arifin HS. 2019. Agricultural biodiversity and economic productivity of the yards in Arguni Bawah, Kaimana District, West Papua Province, Indonesia. Biodiversitas 20: 1020-1026. Papua Island, Indonesia has abundance of agricultural biological resources. This is reflected in a variety of agricultural products in different parts of the area and in the use of yards, which is not fully utilized. This study was aimed at mapping the agricultural biodiversity of the yards, as well as measuring their economic productivity. The study area was Arguni Bawah Sub-district, Kaimana District, West Papua Province, Indonesia, and the study was conducted from November 2017 to March 2018. The average number of plant species recorded from yards were 13, number of individuals being 72 in an average yard size of 696 m2. The smallest yard was 231 m2 with only three species and 4 individuals. The maximum number of species found were 26 with 267 individuals. The maximum size of yards studied was 3,000 m2. There are five functional types of plants with high density and SDR, there are the species producing starch (taro and banana), vegetables (gedi and spinach), fruits (banana) and ornamentals, among others. The Shanon-Wiener diversity index (H´) ranged from 1.8 to 2.5, indicating that the biodiversity of plant species in the yards may be interpreted as abundant or moderate. The evaluation of economic productivity showed that average cash income received by households from yards was Rp.7,693,000.
Diversity of aquatic macrophytes in Balili River, La Trinidad, Benguet, Philippines as potential phytoremediators
Authors: NAPALDET JONES T , BUOT INOCENCIO  JR. E
Published: March 2019

Abstract. Napaldet JT, Buot JR IE. 2019. Diversity of aquatic macrophytes in Balili River, La Trinidad, Benguet, Philippines as potential phytoremediators. Biodiversitas 20: 1048-1054.  Phytoremediation, an emerging, low-cost, aesthetically pleasing technology, is now being eyed for restoration of Balili River, Philippines. But first, there is a need to identify plants ideal for its phytoremediation. Macrophytes in the main stream, Bolo Creek and Mamaga Creek of Balili River were inventoried in this study with focus on determining macrophytes that were local, dominant (=fast growing), have high biomass and deep root systems – traits ideal for phytoremediation. A total of 38 species of macrophytes under 37 genera and 19 families were inventoried. This number is much higher than the previous inventory which recorded only 9 species. Consequently, diversity indices recorded in this study is much higher. The difference could be attributed to the time of sampling wherein the previous inventory was done in July, a rainy month, in contrast with this in April, the summer month at which the river is at its lowest level. At this period, much of the littoral zone is exposed for colonization of plants known to be easily dispersed and germinate such as Asteraceae species. In terms of species richness, family Asteraceae was the most represented with 11 species but in terms of dominance, the story was different. Dominant macrophytes of Balili River were species of family Poaceae (Pennisetum purpureum, Eleusine indica and Cynodon dactylon), Amaranthaceae (Amaranthus spinosus and Alternanthera sessilis), Solanaceae (Solanum americanum) and Commelinaceae (Commelina diffusa). Local macrophytes with potential for phytoremediation were P. purpureum, E. indica, A. spinosus and S. americanum.
Vegetation analysis of non-tidal swampland in South Sumatra, Indonesia and its carrying capacity for Pampangan buffalo pasture
Authors: MUHAKKA MUHAKKA , SUWIGNYO RUJITO AGUS , BUDIANTA DEDIK , YAKUP YAKUP
Published: March 2019

Abstract. Muhakka, Suwignyo RA, Budianta D, Yakup. 2019. Vegetation analysis of non-tidal swampland in South Sumatra, Indonesia and its carrying capacity for Pampangan buffalo pasture. Biodiversitas 20: 1077-1086. In Indonesia, non-tidal swampland area is 13.27 million ha, only 4 million ha has been developed with details of 2.6 million ha that managed by the public and the private sector and 1.3 million ha with government assistance. This study aims to analyze vegetation structure of non-tidal swampland in Pulau Layang Village, Ogan Komering Ilir District, South Sumatra, Indonesia and Rambutan Village, Banyuasin District, South Sumatra, Indonesia and to examine its carrying capacity for Pampangan buffalo pasture. Methods used were by the combination of direct observation, survey using plot sampling with total 50 observation plots, and measurements to determine forage production using Halls method. The results show that there 19 forage species were in two studied areas which are potential as Pampangan buffalo feed. Species with the highest Important Value Index were Purun tikus (Eleocharis .dulcis) with 89.71% and Kumpai padi (Oryza. rufipogon) with 54.08%. The production of fresh forage and dry matter in the wet season in Pulau Layang was 6.90 tons ha-1 year-1 and 1.27 tons ha-1 year-1, respectively, whereas in Rambutan they were 3.68 tons ha-1 year-1 and 0.91 tons ha-1 year-1, respectively. The production of fresh forage and dry matter in the dry season in Pulau Layang was 4.86 tons ha-1 year-1 and 0.99 tons ha-1 year-1, respectively, while in Rambutan they were 2.52 tons ha-1 year-1 and 0,71 tons ha-1 year-1, respectively. The pasture carrying capacity in Pulau Layang in the wet season was 3.66 AU (Animal Unit) ha-1 year-1 and in the dry season, it was 2.85 AU ha-1 year-1, while in Rambutan Village it was 2.61 AU ha-1 year-1 and 2.04 AU ha-1 year-1, respectively. There were six species of forage with high production, namely Kumpai tembaga (Hymenachne acutigluma) Kumpai padi (Oryza rupifogon), Kumpai minyak (Hymenachne amplexicaulis), Are bolong (Polygonum barbatum L), Bento rayap (Leersia hexandra) and Purun tikus (Eleocharis dulcis). It is estimated that there still can be added buffalo cattle as much as 0.31 AU ha-1 year-1 in Pulau Layang Village so 155 buffaloes and 0.59 AU ha-1 year-1 in Rambutan Village.709 buffaloes
Metagenomic analysis of microbial community in over-fermented tempeh
Authors: PANGASTUTI ARTINI , ALFISAH RIZAL KHOIRUN , ISTIANA NUR IFFAH , SARI SITI LUSI ARUM , SETYANINGSIH RATNA , SUSILOWATI ARI , PURWOKO TJAHJADI
Published: March 2019

Abstract. Pangastuti A, Alfisah RK, Istiana NI, Sari SLA, Setyaningsih R, Susilowati A, Purwoko T. 2019. Metagenomic analysis of microbial community in over-fermented tempeh. Biodiversitas 20: 1106-1114. Tempeh is a traditional Indonesian food which is made from soybeans through a fermentation process using Rhizopus as a starter culture. Tempeh is now considered as a functional food with many beneficial effects to human health beyond its nutritional value. The microbial community during the further fermentation process of tempeh give typical characteristic taste and flavor. Therefore the over-fermented tempeh is used as a flavoring in some dishes. Metagenomic analysis needed to know the involvement of microbial communities since most of the microbes involved in further fermentation process are unculturable. This research aimed to study the diversity of the microbial community in the over-fermented tempeh (72 hours) using the metagenomic analysis. Seventeen OTUs of fungi in over-fermented tempeh were detected. Among them, 9 OTUs had significant abundance: six species were identified as Tryblidiopsis sichuanensis, Candida sp.2_1., Kluyveromyces marxianus, Trichosporon asahii, Trichosporon gracile, and Trichosporon ovoides, one species was identified in the order level Mucorales, and two fungi species could not be determined. Species of the order Mucorales was the dominant species in over-fermented tempeh (72 hours) with a relative abundance of 62.46%, followed by Kluyveromyces marxianus with a relative abundance of 3698%. Meanwhile, 132 OTUs of the bacterial community was detected, the predominant 10 genera were Chryseobacterium, Lactococcus, Lactobacillus, Streptococcus, Acetobacter, Novosphingobium, Comamonas, Escherichia, Klebsiella, and Stenotrophomonas, in which Lactobacillus agilis, Lactococcus sp., and Klebsiella sp., were most abundant with relative abundance of 27%, 26.3%, and 13% respectively.