bluuebunny/crossref_metadata_2025 huggingface dataset:
Geographical Detection of Traffic Accidents Spatial Stratified Heterogeneity and Influence Factors
Authors: Zhang Yuhuan , Lu Huapu , Qu Wencong
Published: January 2020

The purpose of this paper is to investigate the existence of stratification heterogeneity in traffic accidents in Shenzhen, what factors influence the casualties, and the interaction of those factors. Geographical detection methods are used for the analysis of traffic accidents in Shenzhen. Results show that spatial stratification heterogeneity does exist, and the influencing factors of fatalities and injuries are different. The traffic accident causes and types of primary responsible party have a strong impact on fatalities and injuries, followed by zones and time interval. However, road factors, lighting, topography, etc., only have a certain impact on fatalities. Drunk driving, speeding over 50%, and overloading are more likely to cause more casualties than other illegal behaviors. Speeding over 50% and speeding below 50% have significant different influences on fatalities, while the influences on injuries are not obvious, and so do drunk driving (Blood Alcohol Concentration ≥ 0.08) and driving under the influence of alcohol (0.08 > Blood Alcohol Concentration ≥ 0.02). Both pedestrians and cyclists violating the traffic law are vulnerable to fatality. Heavy truck overloading is more likely to cause major traffic accidents than minibuses. More importantly, there are nonlinear enhanced interactions between the influencing factors, the combination of previous non-significant factors and other factors can have a significant impact on the traffic accident casualties. The findings could be helpful for making differentiated prevention and control measures for traffic accidents in Shenzhen and the method selection of subsequent research.
The Influence of a Health-Related Fitness Training Program on Motor Performance as Well as Hematological and Biochemical Parameters
Authors: Kostrzewa-Nowak Dorota , Nowakowska Anna , Zwierko Teresa , Rybak Maciej , Nowak Robert
Published: January 2020

The study was aimed at designing a health exercise program appealing to inactive young men, and then testing the men’s metabolic responses to the program using common diagnostic markers of general health. Six men, aged 22–29 years, took a part in training program to increase their motor performance and improve general health conditions. Body composition parameters, clinical chemistry variables (metabolites, albumin, total protein, ferritin, C reactive protein, lipid profile, ions, and selected enzymes activities) and blood morphology parameters were determined. Motor performance measured before and after a 4-month-long macrocycle indicated an increase in endurance, pace, and agility of the participants. Significant differences were found in analyzed enzymes activities. There was a significant increase in C-reactive protein levels from pre- to post-training. Additionally, changes in hematological biomarkers were seen that suggest erythropoiesis might significantly increase, specifically during the last 2-month-long mesocycles. The proposed training program induced small improvements in endurance, pace, and agility. It was also confirmed that changes in aspartate (AST) and alanine (ALT) activities emerge before any increase in creatine kinase (CK) activity that is important in monitoring of the training loads. Observed changes in red blood cell-related parameters suggest increase in erythropoiesis in the second half of the training cycle.
Prevalence and Interpersonal Correlates of Internet Gaming Disorders among Chinese Adolescents
Authors: Yang Xue , Jiang Xuewen , Mo Phoenix Kit-han , Cai Yong , Ma Le , Lau Joseph Tak-fai
Published: January 2020

This study investigated the prevalence and interpersonal correlates of Internet gaming disorders (IGD) among Chinese adolescents. A cross-sectional survey was conducted in two cities (Shanghai and Xi’an) in China. A total of 2666 (Meanage = 12.77 ± 0.75) year-one students from eight middle schools completed a self-reported questionnaire. It tested their levels of IGD, parental psychological control, negative interpersonal events (physical/verbal abuse by parents, verbal abuse by teachers, peer/online bullying), social support from parents/peers, and positive relationships with parents/peers. Results showed that 346 participants (13.0%) were classified as having IGD. Gender, city, single-parent family, family socio-economic status, and mother’s education level were significantly associated with the risk of IGD. Logistic regression analyses with and without controlling for the significant background variables showed that the studied interpersonal variables were significantly associated with IGD, respectively. Forward stepwise logistic regression showed that the significant correlates of IGD included parental psychological control, physical/verbal abuse by parents, verbal abuse by teachers, and peer/online bullying. Results highlight the importance of addressing interpersonal risk factors to reduce adolescent IGD. Limitations and implications of this study are discussed.
“Engaged, Burned Out, or Both?” A Structural Equation Model Testing Risk and Protective Factors for Social Workers in Refugee and Homeless Aid
Authors: Mette Janika , Robelski Swantje , Wirth Tanja , Nienhaus Albert , Harth Volker , Mache Stefanie
Published: January 2020

The present study sheds light on social workers’ working conditions in highly demanding settings and examines the associations between their perceived job demands, resources, resilience, personal burnout, and work engagement. A cross-sectional quantitative online survey was conducted with employees in social work institutions of independent and public sponsors providing help for refugees and homeless persons. The study participants were 243 social workers (68.8% female and 31.3% male) from four federal states in Germany. Correlations between social workers’ job demands, resources, burnout, and work engagement were confirmed in accordance with the Job Demands–Resources model. Results of the structural equation modelling revealed significant positive effects of employees’ job demands on their personal burnout, but no significant effects on their work engagement. The meaning of work as a job resource was significantly positively related to work engagement and negatively related to burnout. Although resilience did not moderate the relationship between employees’ job demands and burnout, it had a significant negative effect on burnout and a positive effect on work engagement. The results indicate a need for the development of health promotion measures for social workers in homeless and refugee aid. Structural approaches should target the reduction of employees’ job demands to diminish their potentially health-depleting effects. Of equal importance, behavioural measures should foster employees’ meaning of work and resilience, since both resources showed beneficial effects on their work engagement and were negatively related to burnout.
Eating Out and Consumers’ Health: Evidence on Obesity and Balanced Nutrition Intakes
Authors: Kim Dahye , Ahn Byeong-il
Published: January 2020

Changes in demographic and socioeconomic characteristics have contributed to an increase in away-from-home food consumption. Although consumers are increasingly demanding higher quality food, unbalanced nutrition intakes and health issues such as obesity remain prominent predicaments. This paper investigates the relationship between the frequency of having Food Away From Home (FAFH), balanced dietary intakes, and obesity (controlling for covariates) among Korean adults aged 19 to 64. Whether there exists a linear relationship between the number of having FAFH and health outcome is investigated and the optimal number of having FAFH that leads to the best health outcome is identified in the study. The results suggest that Food Away From Home generally increases deviations of dietary intakes from the reference intakes and high-frequency FAFH consumers have an elevated chance of being obese (36.22%). However, having FAFH 1–7 times per week is associated with decreased body mass index (BMI) and a lower chance of being obese in comparison to the outcomes of having food at home. The optimal level of consuming FAFH is identified to be 5–7 times per week in terms of BMI and obesity. However, consuming no FAFH is suggested to be the best in terms of balanced nutrition intake.
Asbestos-Related Lung Cancer: A Hospital-Based Case-Control Study in Indonesia
Authors: Suraya Anna , Nowak Dennis , Sulistomo Astrid Widajati , Ghanie Icksan Aziza , Syahruddin Elisna , Berger Ursula , Bose-O’Reilly Stephan
Published: January 2020

Indonesia has limited data on asbestos-related diseases despite abundant use. This study investigated the risk of occupational asbestos exposure for lung cancer development, utilizing a hospital-based case-control study. Subjects were patients who received a thoracic CT scan at Persahabatan Hospital, Jakarta. The cases had primary lung cancer confirmed by histology, the controls were negative for lung cancer. The cumulative occupational asbestos exposure was calculated by multiplying the exposure intensity by the years of exposure. The exposure intensity was obtained by adopting the weighted arithmetic mean value of asbestos exposure from a job-exposure matrix developed in Korea. The primary data analysis was based on logistic regression. The study included 696 subjects, with 336 cases and 360 controls. The chance of lung cancer for subjects exposed to asbestos was doubled (OR = 2.04, 95% CI = 1.21–3.42) compared with unexposed, and subjects with a cumulative asbestos exposure of 10 fiber-years or more even showed an OR of 3.08 (95% CI = 1.01–9.46). The OR of the combined effect between smoking and asbestos was 8.7 (95% CI = 1.71–44.39); the interaction was consistent with an additive and multiplicative risk model. Asbestos exposure is associated with a higher chance of lung cancer. Improved policies are needed to protect the population from asbestos hazards.
(Un)bounded Social Work?—Analysis of Working Conditions in Refugee and Homeless Aid in Relation to Perceived Job Stress and Job Satisfaction
Authors: Robelski Swantje , Mette Janika , Wirth Tanja , Kiepe Niklas , Nienhaus Albert , Harth Volker , Mache Stefanie
Published: January 2020

Little is known about working conditions of social workers providing help in homeless and refugee aid. Therefore, the present study examined their work-related demands, job and personal resources as well as workplace violence, domain-specific demands, and gender-related differences. Job demands and resources were analyzed with regard to their association with job stress and job satisfaction. Two hundred and fifty-three social workers (69.2% female, 30.8% male) from four federal states in Germany (Berlin, Hamburg, Schleswig-Holstein, and Mecklenburg-Western Pomerania) took part in the cross-sectional quantitative online survey that included validated scales and exploratory items especially developed for the target group. Multiple regression analysis showed that resilience as a personal resource was a significant negative predictor of perceived job stress. Emotional demands were positively related with perceived job stress. Meaning of work and social support were strongly associated with job satisfaction. Language and bureaucratic barriers as well as being affected by clients’ experiences were the domain-specific demands named most often. The study offers insights into the work-related demands and resources and their respective impact on perceived job stress and job satisfaction experienced by social workers in refugee and homeless aid. In order to ensure health and safety for this occupational group, health promotion measures focusing on structural aspects are recommended.
Disaster Risk Perception, Sense of Pace, Evacuation Willingness, and Relocation Willingness of Rural Households in Earthquake-Stricken Areas: Evidence from Sichuan Province, China
Authors: Xu Dingde , Qing Chen , Deng Xin , Yong Zhuolin , Zhou Wenfeng , Ma Zhixing
Published: January 2020

Based on survey data from 327 rural households in the areas affected by the Wenchuan Earthquake and Lushan Earthquake in Sichuan Province, this study systematically analyzed disaster risk perception, sense of place, evacuation willingness, and relocation willingness among residents in these earthquake-stricken areas. Further, this study constructed an ordinal logistic regression analysis to probe the correlations between residents’ disaster risk perception or sense of place and evacuation willingness and relocation willingness, respectively. The results showed that (1) faced with the threat of earthquake disasters, residents have a strong willingness to evacuate and relocate. Specifically, 93% and 78% of the residents in the Wenchuan Earthquake and Lushan Earthquake areas were willing to evacuate and relocate, respectively, whereas 4% and 17% of the residents were unwilling to evacuate and relocate, respectively. (2) Place dependence and the severity of disaster occurrence were significantly positively correlated with residents’ evacuation willingness, while the interaction term between place dependence and the severity of disaster occurrence was negatively related to residents’ evacuation willingness. Specifically, when everything else remains constant, every one-unit increase in place dependence and severity corresponds to increases in the odds of willingness to evacuate by factors of 0.042 and 0.051, respectively; every one-unit increase in place dependence × severity corresponds to a decrease in the odds of willingness to evacuation by a factor of 0.004. (3) Place identity was significantly negatively correlated with residents’ relocation willingness, while place dependence and severity of disaster occurrence were positively related to residents’ relocation willingness. The interaction term between place dependence and the severity of disaster occurrence as well as the interaction term between place identity and severity of disaster occurrence were significantly negatively correlated with residents’ relocation willingness. Specifically, every one-unit increase in place identity corresponds to a decrease in the odds of willingness to relocate by a factor of 0.034, while every one-unit increase in place dependence and severity corresponds to increases in the odds of willingness to relocate by factors of 0.041 and 0.028, respectively, and every one-unit increase in place dependence × severity and place identity × severity corresponds to decreases in the odds of willingness to relocate by factors of 0.003 and 0.003, respectively.
Endoplasmic Reticulum Stress Contributes to Indomethacin-Induced Glioma Apoptosis
Authors: Chang Cheng-Yi , Li Jian-Ri , Wu Chih-Cheng , Wang Jiaan-Der , Liao Su-Lan , Chen Wen-Ying , Wang Wen-Yi , Chen Chun-Jung
Published: January 2020

The dormancy of cellular apoptotic machinery has been highlighted as a crucial factor in therapeutic resistance, recurrence, and poor prognosis in patients with malignancy, such as malignant glioma. Increasing evidence indicates that nonsteroidal anti-inflammatory drugs (NSAIDs) confer chemopreventive effects, and indomethacin has been shown to have a novel chemotherapeutic application targeting glioma cells. To extend these findings, herein, we studied the underlying mechanisms of apoptosis activation caused by indomethacin in human H4 and U87 glioma cells. We found that the glioma cell-killing effects of indomethacin involved both death receptor- and mitochondria-mediated apoptotic cascades. Indomethacin-induced glioma cell apoptosis was accompanied by a series of biochemical changes, including reactive oxygen species generation, endoplasmic reticulum (ER) stress, apoptosis signal-regulating kinase-1 (Ask1) activation, p38 hyperphosphorylation, protein phosphatase 2A (PP2A) activation, Akt dephosphorylation, Mcl-1 and FLICE-inhibiting protein (FLIP) downregulation, Bax mitochondrial distribution, and caspases 3/caspase 8/caspase 9 activation. Data on pharmacological inhibition related to oxidative stress, ER stress, free Ca2+, and p38 revealed that the axis of oxidative stress/ER stress/Ask1/p38/PP2A/Akt comprised an apoptotic cascade leading to Mcl-1/FLIP downregulation and glioma apoptosis. Since indomethacin is an emerging choice in chemotherapy and its antineoplastic effects have been demonstrated in glioma tumor-bearing models, the findings further strengthen the argument for turning on the aforementioned axis in order to activate the apoptotic machinery of glioma cells.
Underlying Histopathology Determines Response to Oxidative Stress in Cultured Human Primary Proximal Tubular Epithelial Cells
Authors: Khan Muhammad Ali , Wang Xiangju , Giuliani Kurt T.K. , Nag Purba , Grivei Anca , Ungerer Jacobus , Hoy Wendy , Healy Helen , Gobe Glenda , Kassianos Andrew J.
Published: January 2020

Proximal tubular epithelial cells (PTEC) are key players in the progression of kidney diseases. PTEC studies to date have primarily used mouse models and transformed human PTEC lines. However, the translatability of these models to human kidney disease has been questioned. In this study, we investigated the phenotypic and functional response of human primary PTEC to oxidative stress, an established driver of kidney disease. Furthermore, we examined the functional contribution of the underlying histopathology of the cortical tissue used to generate our PTEC. We demonstrated that human primary PTEC from both histologically ‘normal’ and ‘diseased’ cortical tissue responded to H2O2-induced oxidative stress with significantly elevated mitochondrial superoxide levels, DNA damage, and significantly decreased proliferation. The functional response of ‘normal’ PTEC to oxidative stress mirrored the reported pathogenesis of human kidney disease, with significantly attenuated mitochondrial function and increased cell death. In contrast, ‘diseased’ PTEC were functionally resistant to oxidative stress, with maintenance of mitochondrial function and cell viability. This selective survival of ‘diseased’ PTEC under oxidizing conditions is reminiscent of the in vivo persistence of maladaptive PTEC following kidney injury. We are now exploring the impact that these differential PTEC responses have in the therapeutic targeting of oxidative stress pathways.
Challenges in Using Circulating Micro-RNAs as Biomarkers for Cardiovascular Diseases
Authors: Felekkis Kyriacos , Papaneophytou Christos
Published: January 2020

Micro-RNAs (miRNAs) play a pivotal role in the development and physiology of the cardiovascular system while they have been associated with multiple cardiovascular diseases (CVDs). Several cardiac miRNAs are detectable in circulation (circulating miRNAs; c-miRNAs) and are emerging as diagnostic and therapeutic biomarkers for CVDs. c-miRNAs exhibit numerous essential characteristics of biomarkers while they are extremely stable in circulation, their expression is tissue-/disease-specific, and they can be easily detected using sequence-specific amplification methods. These features of c-miRNAs are helpful in the development of non-invasive assays to monitor the progress of CVDs. Despite significant progress in the detection of c-miRNAs in serum and plasma, there are many contradictory publications on the alterations of cardiac c-miRNAs concentration in circulation. The aim of this review is to examine the pre-analytical and analytical factors affecting the quantification of c-miRNAs and provide general guidelines to increase the accuracy of the diagnostic tests in order to improve future research on cardiac c-miRNAs.
Unravelling the Roles of Nitrogen Nutrition in Plant Disease Defences
Authors: Sun Yuming , Wang Min , Mur Luis Alejandro Jose , Shen Qirong , Guo Shiwei
Published: January 2020

Nitrogen (N) is one of the most important elements that has a central impact on plant growth and yield. N is also widely involved in plant stress responses, but its roles in host-pathogen interactions are complex as each affects the other. In this review, we summarize the relationship between N nutrition and plant disease and stress its importance for both host and pathogen. From the perspective of the pathogen, we describe how N can affect the pathogen’s infection strategy, whether necrotrophic or biotrophic. N can influence the deployment of virulence factors such as type III secretion systems in bacterial pathogen or contribute nutrients such as gamma-aminobutyric acid to the invader. Considering the host, the association between N nutrition and plant defence is considered in terms of physical, biochemical and genetic mechanisms. Generally, N has negative effects on physical defences and the production of anti-microbial phytoalexins but positive effects on defence-related enzymes and proteins to affect local defence as well as systemic resistance. N nutrition can also influence defence via amino acid metabolism and hormone production to affect downstream defence-related gene expression via transcriptional regulation and nitric oxide (NO) production, which represents a direct link with N. Although the critical role of N nutrition in plant defences is stressed in this review, further work is urgently needed to provide a comprehensive understanding of how opposing virulence and defence mechanisms are influenced by interacting networks.
GRK2-Mediated Crosstalk Between β-Adrenergic and Angiotensin II Receptors Enhances Adrenocortical Aldosterone Production In Vitro and In Vivo
Authors: Pollard Celina , Ghandour Jennifer , Cora Natalie , Perez Arianna , Parker Barbara , Desimine Victoria , Wertz Shelby , Pereyra Janelle , Ferraino Krysten , Patel Jainika , Lymperopoulos Anastasios
Published: January 2020

Aldosterone is produced by adrenocortical zona glomerulosa (AZG) cells in response to angiotensin II (AngII) acting through its type I receptors (AT1Rs). AT1R is a G protein-coupled receptor (GPCR) that induces aldosterone via both G proteins and the adapter protein βarrestin1, which binds the receptor following its phosphorylation by GPCR-kinases (GRKs) to initiate G protein-independent signaling. β-adrenergic receptors (ARs) also induce aldosterone production in AZG cells. Herein, we investigated whether GRK2 or GRK5, the two major adrenal GRKs, is involved in the catecholaminergic regulation of AngII-dependent aldosterone production. In human AZG (H295R) cells in vitro, the βAR agonist isoproterenol significantly augmented both AngII-dependent aldosterone secretion and synthesis, as measured by the steroidogenic acute regulatory (StAR) protein and CYP11B2 (aldosterone synthase) mRNA inductions. Importantly, GRK2, but not GRK5, was indispensable for the βAR-mediated enhancement of aldosterone in response to AngII. Specifically, GRK2 inhibition with Cmpd101 abolished isoproterenol’s effects on AngII-induced aldosterone synthesis/secretion, whereas the GRK5 knockout via CRISPR/Cas9 had no effect. It is worth noting that these findings were confirmed in vivo, since rats overexpressing GRK2, but not GRK5, in their adrenals had elevated circulating aldosterone levels compared to the control animals. However, treatment with the β-blocker propranolol prevented hyperaldosteronism in the adrenal GRK2-overexpressing rats. In conclusion, GRK2 mediates a βAR-AT1R signaling crosstalk in the adrenal cortex leading to elevated aldosterone production. This suggests that adrenal GRK2 may be a molecular link connecting the sympathetic nervous and renin-angiotensin systems at the level of the adrenal cortex and that its inhibition might be therapeutically advantageous in hyperaldosteronism-related conditions.
Proteomics-Based Evidence for a Pro-Oncogenic Role of ESRP1 in Human Colorectal Cancer Cells
Authors: Ala Ugo , Manco Marta , Mandili Giorgia , Tolosano Emanuela , Novelli Francesco , Provero Paolo , Altruda Fiorella , Fagoonee Sharmila
Published: January 2020

The RNA-binding protein, Epithelial Splicing Regulatory Protein 1 (ESRP1) can promote or suppress tumorigenesis depending on the cell type and disease context. In colorectal cancer, we have previously shown that aberrantly high ESRP1 expression can drive tumor progression. In order to unveil the mechanisms by which ESRP1 can modulate cancer traits, we searched for proteins affected by modulation of Esrp1 in two human colorectal cancer cell lines, HCA24 and COLO320DM, by proteomics analysis. Proteins hosted by endogenous ESRP1 ribonucleoprotein complex in HCA24 cells were also analyzed following RNA-immunoprecipitation. Proteomics data were complemented with bioinformatics approach to exploit publicly available data on protein-protein interaction (PPI). Gene Ontology was analysed to identify a common molecular signature possibly explaining the pro-tumorigenic role of ESRP1. Interestingly, proteins identified herein support a role for ESRP1 in response to external stimulus, regulation of cell cycle and hypoxia. Our data provide further insights into factors affected by and entwined with ESRP1 in colorectal cancer.
Bacteria from the Midgut of Common Cockchafer (Melolontha melolontha L.) Larvae Exhibiting Antagonistic Activity Against Bacterial Symbionts of Entomopathogenic Nematodes: Isolation and Molecular Identification
Authors: Skowronek Marcin , Sajnaga Ewa , Pleszczyńska Małgorzata , Kazimierczak Waldemar , Lis Magdalena , Wiater Adrian
Published: January 2020

The mechanisms of action of the complex including entomopathogenic nematodes of the genera Steinernema and Heterorhabditis and their mutualistic partners, i.e., bacteria Xenorhabdus and Photorhabdus, have been well explained, and the nematodes have been commercialized as biological control agents against many soil insect pests. However, little is known regarding the nature of the relationships between these bacteria and the gut microbiota of infected insects. In the present study, 900 bacterial isolates that were obtained from the midgut samples of Melolontha melolontha larvae were screened for their antagonistic activity against the selected species of the genera Xenorhabdus and Photorhabdus. Twelve strains exhibited significant antibacterial activity in the applied tests. They were identified based on 16S rRNA and rpoB, rpoD, or recA gene sequences as Pseudomonas chlororaphis, Citrobacter murliniae, Acinetobacter calcoaceticus, Chryseobacterium lathyri, Chryseobacterium sp., Serratia liquefaciens, and Serratia sp. The culture filtrate of the isolate P. chlororaphis MMC3 L3 04 exerted the strongest inhibitory effect on the tested bacteria. The results of the preliminary study that are presented here, which focused on interactions between the insect gut microbiota and mutualistic bacteria of entomopathogenic nematodes, show that bacteria inhabiting the gut of insects might play a key role in insect resistance to entomopathogenic nematode pressure.
Release of Cholecystokinin from Rat Intestinal Mucosal Cells and the Enteroendocrine Cell Line STC-1 in Response to Maleic and Succinic Acid, Fermentation Products of Alcoholic Beverages
Authors: Egberts Jan-Hendrik , Raza Ghulam Shere , Wilgus Cornelia , Teyssen Stefan , Kiehne Karlheinz , Herzig Karl-Heinz
Published: January 2020

Alcoholic beverages stimulate pancreatic enzyme secretions by inducing cholecystokinin (CCK) release. CCK is the major stimulatory hormone of pancreatic exocrine secretions, secreted from enteroendocrine I-cells of the intestine. Fermentation products of alcoholic beverages, such as maleic and succinic acids, influence gastric acid secretions. We hypothesize that maleic and succinic acids stimulate pancreatic exocrine secretions during beer and wine ingestion by increasing CCK secretions. Therefore, the effects of maleic and succinic acids on CCK release were studied in duodenal mucosal cells and the enteroendocrine cell line STC-1. Mucosal cells were perfused for 30 min with 5 min sampling intervals, STC-1 cells were studied under static incubation for 15 min, and supernatants were collected for CCK measurements. Succinate and maleate-induced CCK release were investigated. Succinate and maleate doses dependently stimulated CCK secretions from mucosal cells and STC-1 cells. Diltiazem, a calcium channel blocker, significantly inhibited succinate and maleate-induced CCK secretions from mucosal cells and STC-1 cells. Maleate and succinate did not show cytotoxicity in STC-1 cells. Our results indicate that succinate and maleate are novel CCK-releasing factors in fermented alcoholic beverages and could contribute to pancreatic exocrine secretions and their pathophysiology.
Structural, Molecular, and Functional Alterations of the Blood-Brain Barrier during Epileptogenesis and Epilepsy: A Cause, Consequence, or Both?
Authors: Löscher Wolfgang , Friedman Alon
Published: January 2020

The blood-brain barrier (BBB) is a dynamic, highly selective barrier primarily formed by endothelial cells connected by tight junctions that separate the circulating blood from the brain extracellular fluid. The endothelial cells lining the brain microvessels are under the inductive influence of neighboring cell types, including astrocytes and pericytes. In addition to the anatomical characteristics of the BBB, various specific transport systems, enzymes and receptors regulate molecular and cellular traffic across the BBB. While the intact BBB prevents many macromolecules and immune cells from entering the brain, following epileptogenic brain insults the BBB changes its properties. Among BBB alterations, albumin extravasation and diapedesis of leucocytes from blood into brain parenchyma occur, inducing or contributing to epileptogenesis. Furthermore, seizures themselves may modulate BBB functions, permitting albumin extravasation, leading to activation of astrocytes and the innate immune system, and eventually modifications of neuronal networks. BBB alterations following seizures are not necessarily associated with enhanced drug penetration into the brain. Increased expression of multidrug efflux transporters such as P-glycoprotein likely act as a ‘second line defense’ mechanism to protect the brain from toxins. A better understanding of the complex alterations in BBB structure and function following seizures and in epilepsy may lead to novel therapeutic interventions allowing the prevention and treatment of epilepsy as well as other detrimental neuro-psychiatric sequelae of brain injury.
Correction: Bocheńska, K. et al. Lysosome Alterations in the Human Epithelial Cell Line HaCaT and Skin Specimens: Relevance to Psoriasis. Int. J. Mol. Sci. 2019, 20, 2255
Authors: Bocheńska Katarzyna , Moskot Marta , Malinowska Marcelina , Jakóbkiewicz-Banecka Joanna , Szczerkowska-Dobosz Aneta , Purzycka-Bohdan Dorota , Pleńkowska Joanna , Słomiński Bartosz , Gabig-Cimińska Magdalena
Published: January 2020

The authors wish to make the following corrections to this paper [...]
TBX1 and Basal Cell Carcinoma: Expression and Interactions with Gli2 and Dvl2 Signaling
Authors: Caprio Cinzia , Varricchio Silvia , Bilio Marchesa , Feo Federica , Ferrentino Rosa , Russo Daniela , Staibano Stefania , Alfano Daniela , Missero Caterina , Ilardi Gennaro , Baldini Antonio
Published: January 2020

Early events of basal cell carcinoma (BCC) tumorigenesis are triggered by inappropriate activation of SHH signaling, via the loss of Patched1 (Ptch1) or by activating mutations of Smoothened (Smo). TBX1 is a key regulator of pharyngeal development, mainly through expression in multipotent progenitor cells of the cardiopharyngeal lineage. This transcription factor is connected to several major signaling systems, such as FGF, WNT, and SHH, and it has been linked to cell proliferation and to the regulation of cell shape and cell dynamics. Here, we show that TBX1 was expressed in all of the 51 BCC samples that we have tested, while in healthy human skin it was only expressed in the hair follicle. Signal intensity and distribution was heterogeneous among tumor samples. Experiments performed on a cellular model of mouse BCC showed that Tbx1 is downstream to GLI2, a factor in the SHH signaling, and that, in turn, it regulates the expression of Dvl2, which encodes an adaptor protein that is necessary for the transduction of WNT signaling. Consistently, Tbx1 depletion in the cellular model significantly reduced cell migration. These results suggest that TBX1 is part of a core transcription network that promotes BCC tumorigenesis.
Serum Zinc Level and non-Protein Respiratory Quotient in Patients with Chronic Liver Diseases
Authors: Nishikawa Hiroki , Takata Ryo , Enomoto Hirayuki , Yoh Kazunori , Iwata Yoshinori , Sakai Yoshiyuki , Kishino Kyohei , Shimono Yoshihiro , Ikeda Naoto , Takashima Tomoyuki , Aizawa Nobuhiro , Hasegawa Kunihiro , Ishii Noriko , Yuri Yukihisa , Nishimura Takashi , Iijima Hiroko , Nishiguchi Shuhei
Published: January 2020

We sought to clarify the correlation between non-protein respiratory quotient (npRQ) in indirect calorimetry and serum zinc (Zn) level in chronic liver diseases (CLDs, n = 586, 309 liver cirrhosis (LC) patients, median age = 63 years). Clinical parameters potentially linked to npRQ <0.85 (best cutoff point for the prognosis in LC patients) were also examined in receiver operating characteristic curve (ROC) analyses. The median npRQ was 0.86. The median serum Zn level was 64 μg/dL. The median npRQ in patients with non-LC, Child–Pugh A, Child–Pugh B and Child–Pugh C were 0.89, 0.85, 0.83 and 0.82 (overall p < 0.0001)). The median serum Zn level in patients with npRQ <0.85 (58 μg/dL) was significantly lower than that in patients with npRQ ≥ 0.85 (68 μg/dL) (p < 0.0001). The correlation coefficient (r) between npRQ level and serum Zn level for all cases was 0.40 (p < 0.0001). Similar tendencies were observed in all subgroup analyses. The highest correlation coefficient between serum Zn level and npRQ was found in patients with Child–Pugh C (n = 22, r = 0.69). In ROC analyses for npRQ <0.85, serum Zn level had the highest area under the ROC (AUC) among baseline laboratory parameters (AUC = 0.69). In conclusion, serum Zn level can be helpful for npRQ in patients with CLDs.
Discrete Element Simulation of Orthogonal Machining of Soda-Lime Glass with Seed Cracks
Authors: Yang Guang , Alkotami Hazem , Lei Shuting
Published: January 2020

Demands for producing high quality glass components have been increasing due to their superior mechanical and optical properties. However, due to their high hardness and brittleness, they present great challenges to researchers when developing new machining processes. In this work, the discrete element method (DEM) is used to simulate orthogonal machining of synthetic soda-lime glass workpieces that are created using a bonded particle model and installed with four different types of seed cracks. The effects of these seed cracks on machining performance are studied and predicted through the DEM simulation. It is found that cutting force, random cracks, and surface roughness are reduced by up to 90%, 74%, and 47%, respectively, for the workpieces with seed cracks compared to the regular ones. The results show that high performance machining through DEM simulation can be achieved with optimal seed cracks.
Reply to George S. Ford’s ‘A Counterfactual Impact Analysis of Fair Use Policy on Copyright Related Industries in Singapore: A Critical Review’
Authors: Ghafele Roya
Published: January 2020

Ford’s ‘Comments (Laws 2018, 7(4), 34; https://doi.org/10.3390/laws7040034, https://www.mdpi.com/2075-471X/7/4/34)’ are biased by a partisan approach to the issues at stake and cannot be based on scientific evidence. The article “A Counterfactual Impact Analysis of Fair Use Policy on Copyright Related Industries in Singapore”, which Gibert and Gafelle wrote together nearly a decade ago, came under heavy criticism by George S. Ford from an organization named the Phoenix Centre for Advanced Legal and Economic Public Policy Studies in an article ‘A Counterfactual Impact Analysis of Fair Use Policy on Copyright Related Industries in Singapore: A Critical Review’. (subsequently ‘the fair use study’) The Fair use study was peer reviewed by LAWS and supports the hypothesis that a more flexible fair use policy is correlated with faster growth rates in private copying technology industries and fewer negative consequences than copyright holders may desire to see. The findings of the Fair use study upset Ford as well as a host of different institutions advocating for copyright owners, such as International Federation of Reproduction Rights Organizations; Motion Picture Association; Publishers Association of Australia; New Zealand Society of Authors or Recorded Music NZ-RMNZ. Ford’s article, however, neither contains novel research, nor is it an effort to update this fairly dated analysis, which reflects data nearly twenty years of age. Rather, it is an unnecessary duplication of an old analysis with only some minor modifications, which serve to show that fair use is actually not beneficial to the economy. At the end of this peculiar exercise, Ford himself admits that this analysis is meaningless. The rest of Ford’s article consists of discussing potential limitations of the Fair use study, in a manner which suggests the authors had never disclosed them (which however they had) and thus is misleading. Ford’s most fundamental point of criticism is hinged on a supposed lack of evidence regarding the parallelism assumption, which he himself admits is impossible to offer. Contrary to Ford’s analysis, the Fair use study has the merit of being fully reproducible, which is not the case for Ford’s article. Also, contrary to Ford’s article, the Fair use study has the advantage of carefully drafted limitations and of offering genuine research insights.
Characterization of Lubricated Friction Behavior of Thermal Spray Steel Coatings in Comparison with Grey Cast Iron
Authors: Lou Ming , Alpas Ahmet T.
Published: January 2020

This work examines friction properties of smooth-honed thermal spray (TS) low carbon steel coatings produced on an Al-9.0% Si alloy using a plasma transferred wire arc (PTWA) method and an AISI 1010 wire used as feedstock in comparison with the ASM type D grey cast iron (CI) samples subjected to the same (smooth) honing process. CI samples prepared using a standard honing process were also tested for comparison. Reciprocating sliding tests were performed using a Cameron–Plint tribometer against CrN-coated counterfaces within a speed range of 0.06–1.20 m/s covering the boundary and mixed lubrication conditions. Stribeck curves were constructed to show the coefficient of friction (COF) variations with the ratio (λ) of lubricant film thickness to composite surface roughness of TS and CI samples at the mid-stroke position where sliding speeds and surface roughnesses were measured. Examination of the Stribeck curves showed that the TS coated surfaces provided lower COF values compared to CI surfaces given the same smooth honing treatment, e.g., for λ = 2.7 a COF of 0.029 was observed for TS and 0.035 for CI, whereas conventional honing of CI provided a COF of 0.047 under the same condition. Metallographic evidence was given for the surface features and formation of tribolayers on the contact surfaces. The arithmetic mean heights of the surfaces, Sa measured after the tests remained similar for the smooth-honed TS and CI samples. The low COF values of the TS samples were discussed in terms of the surface pores generated during their manufacturing process, and the high oil retention depth ratio (Svk/Sk) of the TS coated surfaces due to the presence of these pores.
Repairing Hybrid Mg–Al–Mg Components Using Sustainable Cooling Systems
Authors: Blanco David , Rubio Eva María , Marín Marta María , Davim Joao Paulo
Published: January 2020

This paper focused on the maintenance or repair of holes made using hybrid Mg–Al–Mg components by drilling, using two sustainable cooling techniques (dry machining and cold compressed air) and taking surface roughness on the inside of the holes as the response variable. The novelty of the work is in proving that the repair operations of the multi-material components (magnesium–aluminum–magnesium) and the parts made of aluminum and magnesium (separately) but assembled to form a higher component can be done simultaneously, thus reducing the time and cost of the assembly and disassembly of this type of component. The study is based on a design of experiments (DOE) defined as a product of a full factorial 23 and a block of two factors (3 × 2). Based on our findings, we propose that the analyzed operations are feasible under sustainable conditions and, in particular, under dry machining. Also, the results depend on the machining order.
Spark Plasma Sintering Apparatus Used for High-temperature Compressive Creep Tests
Authors: Ratzker Barak , Kalabukhov Sergey , Frage Nachum
Published: January 2020

Creep is a time dependent, temperature-sensitive mechanical response of a material in the form of continuous deformation under constant load or stress. To study the creep properties of a given material, the load/stress and temperature must be controlled while measuring strain over time. The present study describes how a spark plasma sintering (SPS) apparatus can be used as a precise tool for measuring compressive creep of materials. Several examples for using the SPS apparatus for high-temperature compressive creep studies of metals and ceramics under a constant load are discussed. Experimental results are in a good agreement with data reported in literature, which verifies that the SPS apparatus can serve as a tool for measuring compressive creep strain of materials.
Laser Surface Modification of Aluminium Alloy AlMg9 with B4C Powder
Authors: Sroka Marek , Jonda Ewa , Pakieła Wojciech
Published: January 2020

This paper presents the effects of laser treatment (fiber laser YLS-4000) on the microstructure and selected mechanical properties of the surface layer of AlMg (AlMg9) foundry alloy obtained by alloying with boron carbide (B4C). The correlation between laser alloying process parameters and selected properties of the formed layer was discussed. The studies were supported by microstructural analysis of the remelted zone (RZ), heat affected zone (HAZ), undissolved carbide particles, substrate material, and precipitates formed during rapid solidification. Metallographic investigations of the laser-treated layer were performed using optical microscopy and scanning electron microscopy (SEM). The elemental composition and a detailed analysis of chemical composition in micro-areas were carried out using energy dispersive X-ray spectroscopy (EDS). The remelting thickness, heat-affected zone (HAZ), and amount of base material in surface layers were determined. Microhardness tests were performed on transverse cross-sections of the remelted zone to obtain the hardness profiles in the base material (BM), remelted zone (RZ), and heat affected zone (HAZ). The hardness, roughness, and wear resistance measurements showed that the highest tribological properties of the obtained surface layer were achieved using 0.5 Bar protective gas (Ar) during alloying with B4C powder.
A Novel Design to Enhance the Mechanical Properties in Cu-Bearing Antibacterial Stainless Steel
Authors: Sun Shaoheng , Xue Zhiyong , An Licong , Chen Xiaohua , Liu Yifei
Published: January 2020

A novel method based on nano-scale precipitation hardening has been studied to strengthen copper-bearing ferrite antibacterial stainless steel. Bimodal precipitations can be observed after antibacterial annealing and low temperature aging treatment, which are large rod-shaped precipitates and nano-sized spherical precipitates, respectively. Due to two different morphological precipitates, the strength of the material is significantly improved without sacrificing formability, and at the same time, the excellent antibacterial properties remain. Under low temperature aging treatment, there is no obvious evidence to show the segregation at the interface between the rod-shaped copper precipitation and the matrix due to the low segregation coefficient of copper. The nano-sized copper precipitation uniformly nucleated and distributed on the matrix. The optimized heat treatment process is antibacterial annealing at 800 °C for half an hour followed by one-hour-aging treatment at 550 °C.
Future Infrastructural Replacement Through the Smart Bridge Concept
Authors: Reitsema Albert D. , Luković Mladena , Grünewald Steffen , Hordijk Dick A.
Published: January 2020

Most of the bridges and viaducts in the Netherlands were built in the sixties and seventies of the last century, and an increasing number of them will have to be replaced due to technical or functional reasons. The Netherlands is not an exception, many industrialized countries will face a similar replacement task in the near future. With the increased traffic intensities and the importance of mobility, the design and construction strategies for new bridges have to be different from that in the past. New methods need to ensure that traffic hindrance due to construction works and (future) maintenance activities are minimized. At the Delft University of Technology, a SMART bridge concept is being developed for fast and hindrance-free infrastructural replacement. The optimal advantage is achieved by utilizing innovative but proven technologies, and by bringing academic research into practice. A combination of recent innovations in construction technology, such as advanced cementitious materials (ACM), structural health monitoring (SHM) techniques, advanced design methods (ADM), and accelerated bridge construction (ABC) is being used. These innovations represent a step towards the next generation of infrastructure where fast construction, intelligent bridge design, sustainability, zero-energy, no/low maintenance, and aesthetics are key features.
Influence of the Elastoplastic Strain on Fatigue Durability Determined with the Use of the Spectral Method
Authors: Böhm Michał , Kowalski Mateusz , Niesłony Adam
Published: January 2020

The paper presents experimental static and fatigue tests results under random loading conditions for the bending of 0H18N9 steel. The experimental results were used in performing calculations, according to the theoretical assumptions of the spectral method of fatigue life assessment, including elastoplastic deformations. The presented solution extends the use of the spectral method for material fatigue life assessment, in terms of loading conditions, above Hooke’s law theorem. The work includes computational verification of the proposal to extend the applicability of the spectral method of determining fatigue life for the range of elastoplastic deformations. One of the aims of the proposed modification was to supplement the stress amplitudes used to calculate the probability density function of the power spectral density of the signal with correction, due to the plastic deformation and its use for notched elements. The authors have tested the method using four of the most popular probability density functions used in commercial software. The obtained results of comparisons between the experimental and calculation results show that the proposed algorithm, tested using the Dirlik, Benasciutti–Tovo, Lalanne, and Zhao–Baker models, does not overestimate fatigue life, which means that the calculations are on the safe side. The obtained results prove that the elastoplastic deformations can be applied within the frequency domain for fatigue life calculations.