bluuebunny/crossref_metadata_2025 huggingface dataset:
Selenium Improves the Nutritional and Antioxidant Properties of Oregano (Origanum vulgare L.) Grown in Hydroponics
Authors: Skrypnik Liubov , Feduraev Pavel , Golubkina Nadezhda , Maslennikov Pavel , Antipina Maria , Katserov Dmitriy , Nebreeva Sofia , Murariu Otilia Cristina , Tallarita Alessio Vincenzo , Caruso Gianluca
Published: December 2024

Oregano (Origanum vulgare L.) is one of the most commercially popular aromatic spices which has long been used in folk medicine as a medicinal plant due to the presence of a wide group of bioactive secondary metabolites. The experimental data available to date indicate that plants of individual species within the Lamiaceae family implement different strategies for the absorption and distribution of selenium (Se) and differ in their metabolic response to Se treatment. In this regard, the present study investigated the effect of Se application (in the form of sodium selenate) at various concentrations (2.0, 5.0, 10.0, 20.0, and 40.0 μM) on the growth and accumulation of Se in oregano plant shoots and roots, as well as on nutritional components and secondary metabolites in shoots. The addition of Se to the nutrient solution at concentrations of 2–20 μM did not adversely affect the growth and accumulation of biomass in the oregano plants, which were characterized by a significant ability to transfer Se from roots to shoots (the translocation factor was >2). The Se treatment enhanced the nutritional proprieties of the oregano and, particularly, it stimulated the accumulation of carbohydrates and proteins (by 30 and 17%) and minerals (calcium and manganese). The total contents of phenolic compounds, flavonoids, hydroxycinnamic acids, luteolin-7-glucoside and its derivatives, catechin, 3,4-dihydroxybenzoic acid, rosmarinic acid and oleanolic and ursolic acids, as well as the percentage of essential oil, showed a dose-dependent increase in the oregano under the Se treatment. Changes in the relative content of the four main components of oregano essential oil (sabinene, germacrene D, β-caryophyllene, and (Z)-β-ocimene) under the Se treatment were not significant. The relative proportions of β-caryophyllene oxide and terpinen-4-ol increased with Se concentration augmentation in the nutrient solution. The obtained results indicate the prospect of using Se at 5–20 μM concentrations in nutrient solution in hydroponically grown oregano to produce Se-biofortified plants with higher levels of health beneficial compounds and antioxidant activity without yield reduction.
Morphological Characterization of P. serotina subsp. capuli Collected in Ecuador
Authors: Pathania Sakshi , Itle Rachel A. , Chávez Carlos R. , Lema Luis F. , Caballero Verónica L. , Carrasco Juan C. , Chavez Dario J.
Published: December 2024

Prunus serotina (black cherry) is native to America and has five subspecies: serotina, eximia, hirsuta, virens, and capuli. P. serotina subsp. capuli is found in Central and South America with superior fruits found in Ecuador. These have large, juicy, and tasty fruits used for human consumption. They are available in produce markets and have important nutraceutical properties. However, no commercial cultivars of capuli are currently available. The main goal of this research was to understand if different morphological characters can differentiate unique populations of P. serotina subsp. capuli present in Ecuador. Morphological traits (tree, leaf, and flower) of plants grown from the OP seeds of 44 capuli accessions collected from three provinces of Ecuador (Cotopaxi, Chimborazo, and Tungurahua) were characterized in 2019 and 2020. Tree measurements included the number of primary branches and growth habit. Leaf measurements included petiole length, leaf area, leaf height, leaf width, leaf apex angle, and leaf basal angle. Flower measurements included pedicel length, flower width, and flower length. Raceme length, number of racemes per branch, and number of flowers per raceme were also characterized. ANOVA were performed with significant differences observed among capuli accessions for all variables measured. No clear differences were observed across regions with PCA and cluster analysis that may support the presence of different populations.
Urtica dioica L. Leaf Extract Dose-Dependently Modulates Oxidative Stress in the Kidney and Exerts Anti-Fibrotic and Anti-Inflammatory Properties by the Molecular Mechanisms Independent of NRF-2 Signalization Mirroring the Effects of Losartan in SHR
Authors: Vajic Una-Jovana , Mihailovic-Stanojevic Nevena , Karanovic Danijela , Zivotic Maja , Ivanov Milan , Jovovic Djurdjica , Grujic-Milanovic Jelica , Miloradovic Zoran
Published: December 2024

Previously, we confirmed systemic antihypertensive and antioxidant properties of Urtica dioica L. leaf extract (UE) in spontaneously hypertensive rats (SHR). Here, we aimed to evaluate whether UE can alter the NO and Nrf-2 signaling to prevent local oxidative stress and kidney damage in the model of essential hypertension. SHR were divided into five groups: SHRC-control, received 0.5 mL/day of water, SHR+L received 10 mg/kg/day of losartan, SHR+UE10, SHR+UE50, and SHR+UE200 received 10, 50, and 200 mg/kg/day during next 4 weeks. At the end of the experiment, urine samples were collected for albuminuria and nitrate/nitrite assessment. Mean arterial pressure (MAP) was measured, and blood samples were collected for plasma creatinine evaluation. Kidneys were analyzed for nitrate/nitrite, oxidative stress, and target molecules by biochemical, Western blot, and immunofluorescent techniques. Losartan and UE50 significantly reduced MAP, albuminuria, oxidative stress, fibroinflammatory markers, and NRF-2/CAT/SOD signaling, with a significant increase in 6-nitrotryptophan and eNOS expressions compared to control. The effects of UE showed dose dependence. Beneficial effects of UE and losartan were independent of NRF-2 signalization in SHR. Interestingly, all treatments induced the increase in 6-nitrotryptophan expression, thus further studies are needed to elucidate the mechanisms of such nitrated tryptophan.
Analysis of Plant Physiological Parameters and Gene Transcriptional Changes Under the Influence of Humic Acid and Humic Acid-Amino Acid Combinations in Maize
Authors: Decsi Kincső , Ahmed Mostafa , Rizk Roquia , Abdul-Hamid Donia , Tóth Zoltán
Published: December 2024

The study investigated the application of humic acids (HAs) and a combination of humic acids and amino acids (HA+AA) in maize under field conditions. Based on preliminary data in the literature, the aim was to investigate the effects of the two plant conditioning compounds on plant physiological parameters. In addition to measuring plant physiological parameters in the field, a complete transcriptome analysis was performed to determine exactly which genes were expressed after the treatments and in which physiological processes they play a role. Maize plants showed significant positive yield changes after two priming treatments. Genome-wide transcriptomic analysis revealed the activation of photosynthetic and cellular respiration processes, as well as protein synthesis pathways, which explains the increased yield even under extreme precipitation conditions. The results show that the HA treatment helped in water management and increased the chlorophyll content, while the HA+AA treatment led to higher protein and dry matter contents. The post-harvest tests also show that the HA+AA treatment resulted in the highest yield parameters. Functional annotation of the maize super transcriptome revealed genes related to translation processes, photosynthesis, and cellular respiration. The combined pathway analysis showed that the HA and combined treatments activated genes related to photosynthesis, carbon fixation, and cellular respiration, providing valuable in-depth insight into the usefulness of the HA and HA+AA treatments in priming. Based on the studies, we believe that the use of natural-based humic acid plant conditioners may provide a beneficial opportunity to promote renewable, regenerative agriculture.
Cadmium-Induced Oxidative Damage and the Expression and Function of Mitochondrial Thioredoxin in Phascolosoma esculenta
Authors: Gu Shenwei , Zheng Xuebin , Gao Xinming , Liu Yang , Chen Yiner , Zhu Junquan
Published: December 2024

Phascolosoma esculenta is a unique aquatic invertebrate native to China, whose habitat is highly susceptible to environmental pollution, making it an ideal model for studying aquatic toxicology. Mitochondrial thioredoxin (Trx2), a key component of the Trx system, plays an essential role in scavenging reactive oxygen species (ROS), regulating mitochondrial membrane potential, and preventing ROS-induced oxidative stress and apoptosis. This study investigated the toxicity of cadmium (Cd) on P. esculenta and the role of P. esculenta Trx2 (PeTrx2) in Cd detoxification. The results showed that Cd stress altered the activities of T-SOD and CAT, as well as the contents of GSH and MDA in the intestine. After 96 h of exposure, histological damages such as vacuolization, cell necrosis, and mitophagy were observed. Suggesting that Cd stress caused oxidative damage in P. esculenta. Furthermore, with the prolongation of stress time, the expression level of intestinal PeTrx2 mRNA initially increased and then decreased. The recombinant PeTrx2 (rPeTrx2) protein displayed dose-dependent redox activity and antioxidant capacity and enhanced Cd tolerance of Escherichia coli. After RNA interference (RNAi) with PeTrx2, significant changes in the expression of apoptosis-related genes (Caspase-3, Bax, Bcl-2, and Bcl-XL) were observed. Proving that PeTrx2 rapidly responded to Cd stress and played a vital role in mitigating Cd-induced oxidative stress and apoptosis. Our study demonstrated that PeTrx2 is a key factor for P. esculenta to endure the toxicity of Cd, providing foundational data for further exploration of the molecular mechanisms underlying heavy metal resistance in P. esculenta.
Comparative Analysis of Controlled Ovarian Hyperstimulation and Modified Natural Cycle Protocols on Gene Expression and Quality of Oocytes, Zygotes, and Embryos in Assisted Reproductive Technology (ART)
Authors: Dević Pavlić Sanja , Saftić Martinović Lara , Sušanj Šepić Tina , Radojčić Badovinac Anđelka
Published: December 2024

This study investigated the influence of two in vitro fertilization (IVF) protocols—controlled ovarian hyperstimulation (COH) and a modified natural cycle protocol—on gene expression levels (Anti-Müllerian Hormone (AMH), Anti-Müllerian Hormone Receptor Type 2 (AMHAMHR2), Follicle-Stimulating Hormone Receptor (FSHR), and Androgen Receptor (AR)) and the subsequent reproductive outcomes of assisted reproductive technology (ART). Gene expression, as well as oocyte, zygote, and embryo morphological parameters, were analyzed to evaluate the differences between the protocols. Our findings show that AMH expression was significantly associated with successful fertilization, while AMHAMHR2 expression correlated with improved embryo transfer outcomes. The modified natural cycle protocol demonstrated a higher association with the favorable gene expression profiles, particularly for AMH and AMHAMHR2, linked to successful fertilization and embryo transfer, suggesting potential advantages of minimal intervention. However, the overall quality scores for the oocytes, zygotes, and embryos were comparable between the protocols. The trend of a higher transfer success for the natural cycle, though not statistically significant, indicated potential protocol effects on the uterine environment. This study highlights the complexity of ART outcomes and suggests that incorporating gene expression markers with protocol adjustments may optimize individual ART strategies.
Foliar Application of Silicon Influences the Physiological and Epigenetic Responses of Wheat Grown Under Salt Stress
Authors: Tobiasz-Salach Renata , Stadnik Barbara , Mazurek Marzena , Buczek Jan , Leszczyńska Danuta
Published: December 2024

Soil salinity is considered a serious problem that limits agricultural productivity. Currently, solutions are being sought to mitigate the negative impact of salt on economically important crops. The aim of the study was to evaluate the effect of foliar application of silicon (Si) on the physiological and epigenetic responses of wheat grown under salt stress conditions. The experiment with wheat seedlings was established in pots with 200 mM NaCl added. After 7 days, foliar fertilizer (200 g L−1 SiO2) was used at concentrations of 0.05, 0.1 and 0.2%. Physiological parameters were measured three times. The addition of salt caused a significant decrease in the values of the measured parameters in plants of all variants. In plants sprayed with Si fertilizer under salinity conditions, a significant increase in CCI and selected gas exchange parameters (PN, Ci, E, gs) and chlorophyll fluorescence (PI, RC/ABS, FV/Fm, Fv/F0) was observed. Si doses of 0.1 and 0.2% showed a better mitigating effect compared to the dose of 0.05%. The observed effect was maintained over time. The results obtained indicate a positive role for foliar silicon fertilization in mitigating salinity stress in wheat. Epigenetic mechanisms play an important role in regulating gene expression in response to stress. Changes in the status of methylation of the 5′CCGG3′ sequence of the nuclear genome of wheat plants exposed to salinity and treated with Si at different doses were determined by the MSAP approach. The obtained results showed a clear alteration of DNA methylation in plants as a response to experimental factors. The methylation changes were silicon dose-dependent. These modifications may suggest a mechanism for plant adaptation under salt stress after silicon application.
Stress on the Endoplasmic Reticulum Impairs the Photosynthetic Efficiency of Chlamydomonas
Authors: Chen Sa , Li Shuyu , Qian Shiyuan , Xing Jiale , Liao Jingjing , Guo Zhifu
Published: December 2024

Stress on the Endoplasmic reticulum (ER) can severely disrupt cellular function by impairing protein folding and post-translational modifications, thereby leading to the accumulation of poor-quality proteins. However, research on its impact on photosynthesis remains limited. In this study, we investigated the impact of ER stress on the photosynthetic efficiency of Chlamydomonas reinhardtii using pharmacological inducers, tunicamycin (TM) and brefeldin A (BFA), which specifically target the ER. Our measurements of photosynthetic parameters showed that these ER stress-inducing compounds caused a significant decline in photosynthetic efficiency. A proteomic analysis confirmed that TM and BFA effectively induce ER stress, as evidenced by the upregulation of ER stress-related proteins. Furthermore, we observed a widespread downregulation of photosynthesis-related proteins, which is consistent with the results obtained from our measurements of photosynthetic parameters. These findings suggest that the stress on ER has a profound impact on chloroplast function, disrupting photosynthetic processes. This study highlights the critical interdependence between the ER and chloroplasts, and it underscores the broader implications of ER stress on the cellular metabolism and energy efficiency of photosynthetic organisms.
Increased Levels of hsa-miR-199a-3p and hsa-miR-382-5p in Maternal and Neonatal Blood Plasma in the Case of Placenta Accreta Spectrum
Authors: Timofeeva Angelika V. , Fedorov Ivan S. , Nikonets Anastasia D. , Tarasova Alla M. , Balashova Ekaterina N. , Degtyarev Dmitry N. , Sukhikh Gennady T.
Published: December 2024

Despite the increasing number of placenta accreta spectrum (PAS) cases in recent years, its impact on neonatal outcomes and respiratory morbidity, as well as the underlying pathogenetic mechanism, has not yet been extensively studied. Moreover, no study has yet demonstrated the effectiveness of antenatal corticosteroid therapy (CT) for the prevention of respiratory distress syndrome (RDS) in newborns of mothers with PAS at the molecular level. In this regard, microRNA (miRNA) profiling by small RNA deep sequencing and quantitative real-time PCR was performed on 160 blood plasma samples from preterm infants (gestational age: 33–36 weeks) and their mothers who had been diagnosed with or without PAS depending on the timing of the antenatal RDS prophylaxis. A significant increase in hsa-miR-199a-3p and hsa-miR-382-5p levels was observed in the blood plasma of the newborns from mothers with PAS compared to the control group. A clear trend toward the normalization of hsa-miR-199a-3p and hsa-miR-382-5p levels in the neonatal blood plasma of the PAS groups was observed when CT was administered within 14 days before delivery, but not beyond 14 days. Direct correlations were found among the hsa-miR-382-5p level in neonatal blood plasma and the hsa-miR-199a-3p level in the same sample (r = 0.49; p < 0.001), the oxygen requirements in the NICU (r = 0.41; p = 0.001), the duration of the NICU stay (r = 0.31; p = 0.019), and the severity of the newborn’s condition based on the NEOMOD scale (r = 0.36; p = 0.005). Logistic regression models based on the maternal plasma levels of hsa-miR-199a-3p and hsa-miR-382-5p predicted the need for cardiotonic therapy, invasive mechanical ventilation, or high-frequency oscillatory ventilation in newborns during the early neonatal period, with a sensitivity of 95–100%. According to the literary data, these miRNAs regulate fetal organogenesis via IGF-1, the formation of proper lung tissue architecture, surfactant synthesis in alveolar cells, and vascular tone.
Investigating the Iron Plating and Stripping of Anolytes for All-Iron Redox-Flow Batteries
Authors: Engler Marius , Stich Michael , Baumer Christoph , Bund Andreas
Published: December 2024

All-iron redox-flow batteries (AIRFB) are capable of addressing the needs for cost-effective long-term storage of renewable energies. Currently, a major limitation of AIRFB performance is the half-cell reaction of the anolyte utilising the redox couple Fe/Fe2+. In this work, the performance of sulphate and chloride-based iron electrolytes was investigated by combining cyclic voltammetry (CV) and electrochemical quartz crystal microbalance (EQCM). The investigations demonstrate that complexing agents exert a detrimental influence on the kinetics of plating/stripping reactions, resulting in diffusivity reduction, while favouring hydrogen evolution reaction (HER). The coulombic (plating) efficiency was found to be 87.1% at −1.2 V vs. Ag/AgCl (sat’d) at pH 3.5, while the coulombic efficiency in oxidation sweep (stripping) was observed to be 100% in an electrolyte containing 0.8 M FeCl2 and 3 M NH4Cl. In the context of iron deposition, the most crucial factors are the suppression of HER, and the influence of diffusion limitations, as well as the role of additives in this process to achieve a high reversibility. It is evident that the investigated complexing agents of glycine, malic acid and malonic acid are inadequate for battery-compatible, efficient properties, given that the overvoltages for the charge transfer reaction are too high and parasitic HER reduces coulombic efficiencies. Ultimately, the choice of deposition parameters from EQCM and electrolyte composition reduced to 0.8 M FeCl2, and 3 M NH4Cl can optimise the battery efficiencies as such.
Extracellular Volume by Computed Tomography Is Useful for Prediction of Prognosis in Dilated Cardiomyopathy Cases with Heart Failure with Reduced Ejection Fraction
Authors: Yashima Satomi , Takaoka Hiroyuki , Ota Joji , Matsumoto Moe , Nishikawa Yusei , Noguchi Yoshitada , Aoki Shuhei , Yoshida Kazuki , Suzuki Katsuya , Kinoshita Makiko , Sasaki Haruka , Suzuki-Eguchi Noriko , Kanaeda Tomonori , Kobayashi Yoshio
Published: December 2024

Objective: Cardiac computed tomography (CT) helps screen coronary artery stenosis in cases with dilated cardiomyopathy (DCM). Extracellular volume fraction (ECV) analysis has recently been eligible for CT. Method: We evaluated the impact of ECV on the CT to predict the prognosis in DCM patients with heart failure with reduced ejection fraction (HFrEF). Patients or Materials: We analyzed 101 consecutive DCM cases with HFrEF who underwent cardiac CT. All the patients had a lower left ventricular (LV) ejection fraction (LVEF) of less than 40%. We evaluated the effect of ECV to predict the patients’ prognosis. Cardiovascular death, hospitalization due to heart failure, and fatal arrhythmic events were included in the major adverse cardiac events (MACE). Results: MACE occurred in 27 cases (27%). The patients with MACE (27 cases) had an increased ECV on the LVM on the CT (37.2 ± 6.7 vs. 32.2 ± 3.6%, p = 0.0008) compared to the others (74 cases). Based on the receiver operating characteristics curve analysis, the best cutoff value of the ECV on the LVM to predict the MACE was 32.3%. The patients with ECV ≥ 32.3% had significantly higher MACE based on the Kaplan–Meier analysis. The ECV on the LVM was a significant marker to predict MACE based on the univariate Cox proportional hazard model (hazard ratio of 8.00, 95% confidence interval 1.88–33.97, p = 0.0048). Conclusions: ECV by CT is helpful to predict MACE in cases with DCM and HFrEF.
Tailored Therapies for Cardiogenic Shock in Hypertrophic Cardiomyopathy: Navigating Emerging Strategies
Authors: Zakynthinos George E. , Gialamas Ioannis , Tsolaki Vasiliki , Pantelidis Panteleimon , Goliopoulou Athina , Gounaridi Maria Ioanna , Tzima Ioanna , Xanthopoulos Andrew , Kalogeras Konstantinos , Siasos Gerasimos , Oikonomou Evangelos
Published: December 2024

Hypertrophic cardiomyopathy (HCM) is a complex and heterogeneous cardiac disorder, often complicated by cardiogenic shock, a life-threatening condition marked by severe cardiac output failure. Managing cardiogenic shock in HCM patients presents unique challenges due to the distinct pathophysiology of the disease, which includes dynamic left ventricular outflow tract obstruction, diastolic dysfunction, and myocardial ischemia. This review discusses current and emerging therapeutic strategies tailored to address the complexities of HCM-associated cardiogenic shock and other diseases with similar pathophysiology that provoke left ventricular outflow tract obstruction. We explore the role of pharmacological interventions, including the use of vasopressors and inotropes, which are crucial in stabilizing hemodynamics but require careful selection to avoid exacerbating the outflow obstruction. Additionally, the review highlights advancements in mechanical circulatory support devices such as extracorporeal membrane oxygenation (ECMO) and left ventricular assist devices (LVADs), which have become vital in the acute management of cardiogenic shock. These devices provide temporary support and bridge patients to recovery, definitive therapy, or heart transplantation, which remains a critical option for those with end-stage disease. Furthermore, the review delves into the latest research and clinical trials that are refining these therapeutic approaches, ensuring they are optimized for HCM patients. The impact of these treatments on patient outcomes, including survival rates and quality of life, is also critically assessed. In conclusion, this review underscores the importance of a tailored therapeutic approach in managing cardiogenic shock in HCM patients, integrating pharmacological and mechanical support strategies to improve outcomes in this high-risk population.
Trajectory of Urine Parameters by Adding Herbal Kampo Medicine Goreisan to Tolvaptan in Patients with Congestive Heart Failure
Authors: Kakeshita Kota , Imamura Teruhiko , Hida Yuki , Onoda Hiroshi , Koike Tsutomu , Kinugawa Koichiro
Published: December 2024

Background: Even in current guideline-directed medical therapy, including recently introduced vasopressin type 2 receptor antagonist tolvaptan, congestion has not been resolved in patients with heart failure. Kampo medicine goreisan has been receiving considerable attention as an additional therapy for patients who are refractory to conventional diuretics therapy, including tolvaptan. However, the impact of goreisan on urine electrolytes remains uncertain. Methods: Patients with congestive heart failure who received goreisan as an add-on therapy to tolvaptan-incorporated medical therapy were prospectively included. The changes in urine parameters during the first 24 h were assessed as a primary concern. Baseline factors associated with an increase in urine sodium excretion were investigated. Results: A total of 21 patients were included. The median age was 81 (77, 86), and 13 (62%) were men. Twenty-four hours after the initiation of goreisan, urine osmolality decreased significantly, urine sodium level remained unchanged, urine potassium and glucose levels decreased significantly, urine urea nitrogen level tended to decrease, and urine volume tended to increase. The fractional excretion of sodium tended to increase. Baseline plasma B-type natriuretic peptide level had a positive correlation with a change in fractional excretion of sodium from baseline to day 1 (r = 0.52, p = 0.015). Conclusions: Goreisan may increase urine volume via aquaretic and natriuretic effects in patients with congestive heart failure receiving tolvaptan-incorporated medical therapy. Goreisan may have the ability to “modulate” fluid balance depending on congestion status.
Efficacy of Dietary Interventions for Irritable Bowel Syndrome: A Systematic Review and Network Meta-Analysis
Authors: Haghbin Hossein , Hasan Fariha , Gangwani Manesh Kumar , Zakirkhodjaev Nurruddinkhodja , Lee-Smith Wade , Beran Azizullah , Kamal Faisal , Hart Benjamin , Aziz Muhammad
Published: December 2024

Introduction: Irritable bowel syndrome (IBS) is a common condition that alters the quality of life of patients. A variety of dietary interventions have been introduced to address this debilitating condition. The low-FODMAP diet (LFD), gluten-free diet (GFD), and Mediterranean diet are examples showing efficacy. The aim of this network meta-analysis was to compare these interventions to find the best approach. Methods: We performed a systematic review of the available literature through 14 March 2024 in the following databases: Embase, PubMed, MEDLINE OVID, Web of Science, CINAHL Plus, and Cochrane Central. We only included randomized controlled trials (RCTs). We used a random effects model and conducted a direct meta-analysis based on the DerSimonian–Laird approach and a network meta-analysis based on the frequentist approach. Mean differences (MDs) with 95% confidence interval (CI) were calculated. The primary outcomes included IBS quality of life (IBS QOL) and IBS symptom severity scale (IBS-SSS). Results: We finalized 23 studies including 1689 IBS patients. In the direct meta-analysis, there was no statistically significant difference in any IBS score between GFD and either LFD or standard diet. Meanwhile, the LFD was statistically superior to the standard diet in the IBS-SSS (MD: −46.29, CI: −63.72–−28.86, p < 0.01) and IBS QOL (MD: 4.06, CI: 0.72–7.41, p = 0.02). On ranking, the Mediterranean diet showed the greatest improvement in IBS-SSS, IBS-QOL, distension, dissatisfaction, and general life interference, followed by the LFD alone or in combination with the GFD. Conclusions: We demonstrated the efficacy of dietary interventions such as the LFD and Mediterranean diet in improving IBS. There is a need for large RCTs with head-to-head comparisons, particularly for the Mediterranean diet.
The Resilience of Polish Doctors and Their Behavioral Patterns in Coping with Work-Related Stress
Authors: Wojczyk Sławomir , Dąbek Józefa , Sierka Oskar , Gąsior Tomasz
Published: December 2024

Background/Objective: The aim of this study was to assess stress-coping patterns among Polish doctors, taking into account their degree of resilience and sociodemographic characteristics. Methods: This study involved 832 (100%) Polish physicians working in the Silesian Voivodeship, Poland. All respondents gave their informed and voluntary consent to participate. This study was conducted using an original questionnaire and the Resilience Measurement Scale (SPP-25) Stress Coping Inventory (Mini-COPE). Results: Women used the strategies: Seeking Emotional Support and Seeking Instrumental Support significantly more often than men. Positive Re-evaluation strategy was used more often by doctors working in surgical wards, who also declared more frequent use of psychoactive substances. Younger doctors (≤30 years) were characterized by lower mental resilience compared to other age groups and doctors with high psychological resilience were characterized by higher scores in strategies belonging to the group of active coping with stress. Conclusions: The examined doctors with high resilience were mostly characterized by the use of active methods of coping with stress, in contrast to doctors with low resilience. Actions should be taken to increase the awareness of healthcare system employees in the field of active stress coping techniques and their adverse effects on health.
Long-Term Survival of Methotrexate as First-Line Therapy in Rheumatoid Arthritis, Psoriatic Arthritis and Undifferentiated Arthritis
Authors: Perrotta Fabio Massimo , Ambrosino Pasquale , Lubrano Ennio
Published: December 2024

Background/Objectives: In the era of biotechnological drugs, methotrexate (MTX) still represents the first-line treatment in chronic inflammatory arthritis, such as rheumatoid arthritis (RA), psoriatic arthritis (PsA), and undifferentiated arthritis (UA). The aim of our study was to evaluate the persistence of MTX as a first-line treatment in a group of patients with chronic inflammatory arthritis. Methods: We conducted a retrospective analysis of a database of outpatients diagnosed with RA, PsA, or UA who visited our Rheumatology Clinic from January 2014 to January 2022. Key demographic and clinical data, including information on comorbidities and treatments, were routinely collected. Kaplan–Meier (KM) curves were used to determine the persistence of MTX during follow-up. Results: A total of 242 patients with chronic inflammatory arthritis who initiated MTX as first-line therapy and had available clinical data were included. Of these, 130 (53.7%) had RA, 82 (33%) had PsA, and 30 (16.3%) had UA. Overall, the survival rate of MTX at 24 months of follow-up was approximately 60%, while at 48 months and 96 months, it was 40% and 20%, respectively. A statistically significant difference was found between RA and PsA compared to UA (Chi-square test = 14.84; p = 0.001). When comparing the KM survival curves of MTX between male and female patients, obese and non-obese individuals, as well as older (age ≥ 50 years) and younger patients (age < 50 years), no statistically significant differences were observed in any of the comparisons. Conclusions: Our study confirmed the efficacy and overall safety of MTX in RA and PsA, with good persistence even over the long term.
Physical Activity and Frailty Are Impaired in Older Adults with Benign Paroxysmal Positional Vertigo
Authors: Pauwels Sara , Lemkens Nele , Lemmens Winde , Meijer Kenneth , Bijnens Wouter , Meyns Pieter , van de Berg Raymond , Spildooren Joke
Published: December 2024

Background/Objectives: Benign Paroxysmal Positioning Vertigo (BPPV), diagnosed in 46% of older adults with complaints of dizziness, causes movement-related vertigo. This case-control study compared physical activity, frailty and subjective well-being between older adults with BPPV (oaBPPV) and controls. Methods: Thirty-seven oaBPPV (mean age 73.13 (4.8)) were compared to 22 matched controls (mean age 73.5 (4.5)). Physical activity was measured using the MOX accelerometer and the International Physical Activity Questionnaire. Modified Fried criteria assessed frailty. The Dizziness Handicap Inventory, Falls Efficacy Scale, and 15-item Geriatric Depression Scale assessed subjective well-being. A post-hoc sub-analysis compared all variables compared between frail oaBPPV, robust oaBPPV and robust controls. Significance level was set at α = 0.05. Results: oaBPPV were significantly less physically active and were more (pre-)frail (p < 0.001) compared to controls. They performed significantly less active bouts (p = 0.002) and more sedentary bouts (p = 0.002), and a significantly different pattern of physical activity during the day. OaBPPV reported significantly less time in transportation activities (p = 0.003), leisure (p < 0.001), walking (p < 0.001) and moderate-intensity activities (p = 0.004) compared to controls. Frail oaBPPV were even less active (p = 0.01) and experienced more fear of falling (p < 0.001) and feelings of depression (p < 0.001) than robust oaBPPV and controls. Conclusions: BPPV can induce a vicious cycle of fear of provoking symptoms, decreased physical activity, well-being and consequently frailty. It is also possible that frail and less physically active older adults have an increased prevalence of BPPV. Post-treatment follow-up should assess BPPV, frailty and physical activity to determine if further rehabilitation is needed.
Updates on Psoriasis in Special Areas
Authors: Butacu Alexandra-Irina , Toma Cristian , Negulet Iulia-Elena , Manole Ionela , Banica Angela Nina , Plesea Alexandra , Badircea Ioana Alexandra , Iancu Isabela , Tiplica George-Sorin
Published: December 2024

Special areas of involvement in psoriasis include the scalp region, the palms and soles, genital areas, as well as intertriginous sites. The involvement of these topographical regions is associated with important physical and emotional implications, resulting in reduced quality of life, social isolation, and work disability. Palms and soles can be affected as part of the generalized form of psoriasis or can be exclusively affected as palmo-plantar psoriasis. Nail involvement may be encountered in 10–55% of patients with psoriasis, while scalp involvement occurs in 45–56% of individuals with psoriasis. Genital involvement may be the only manifestation of cutaneous psoriasis in 2–5% of patients. Inverse or intertriginous psoriasis represents a special variant of psoriasis as it may mimic and be difficult to differentiate from other dermatological entities that involve the intertriginous skin, such as bacterial or fungal infections, eczema, or lichen planus. Treatment of psoriasis in special areas is challenging due to the facts that special areas are more resistant to standard therapies and are more sensitive to potent local treatments. Biological therapies, proven to be more efficient than standard therapies, are not widely available in the absence of extensive skin involvement. This manuscript aims to provide an up-to-date literature review on psoriasis in special areas, benefiting the everyday clinical practice of physicians in optimizing the evaluation and treatment of their patients.
Three-Dimensional Bone-Image Synthesis with Generative Adversarial Networks
Authors: Angermann Christoph , Bereiter-Payr Johannes , Stock Kerstin , Degenhart Gerald , Haltmeier Markus
Published: December 2024

Medical image processing has been highlighted as an area where deep-learning-based models have the greatest potential. However, in the medical field, in particular, problems of data availability and privacy are hampering research progress and, thus, rapid implementation in clinical routine. The generation of synthetic data not only ensures privacy but also allows the drawing of new patients with specific characteristics, enabling the development of data-driven models on a much larger scale. This work demonstrates that three-dimensional generative adversarial networks (GANs) can be efficiently trained to generate high-resolution medical volumes with finely detailed voxel-based architectures. In addition, GAN inversion is successfully implemented for the three-dimensional setting and used for extensive research on model interpretability and applications such as image morphing, attribute editing, and style mixing. The results are comprehensively validated on a database of three-dimensional HR-pQCT instances representing the bone micro-architecture of the distal radius.
The Development and Application of an Intelligent Assessment and Strategy Implementation System for Non-Intellectual Factors in Mathematics Learning Among Senior High School Students
Authors: Kang Yueyuan , Wang Guangming , Liu Luxuan , Liu Jing , Gao Qianqian
Published: December 2024

Non-intellectual factors in mathematics are key psychological factors that influence students’ cognitive activities and, consequently, their learning efficiency. While the assessment of these factors has gained increasing academic attention, research on the effective use of intelligent tools to assess and improve students’ non-intellectual factors remains insufficient. This study employed intelligent technology to develop the Intelligent Assessment and Strategy Implementation System for Non-intellectual Factors in Mathematics Learning for Primary and Secondary School Students, which integrates an assessment index system, scales, regional norms, and personalized improvement strategies, enabling it to automatically generate bulk reports on students’ non-intellectual factor scores across various dimensions and provide targeted improvement strategies. In order to test its effectiveness, the intelligent system was applied across several provinces, cities, and schools in China. Eleventh-grade students from X Middle School in T City served as a representative case study. The interventions were based on the strategies provided by the system, and the research consistently demonstrated that the “Intelligent Assessment and Strategy Implementation System of Mathematics Non-intellectual Factors for Primary and Secondary School Students” effectively delivers high-precision diagnoses and personalized intervention strategies.
A Novel 10 MW Floating Wind Turbine Platform—SparFloat: Conceptual Design and Dynamic Response Analysis
Authors: Shen Yong , Liu Jian , Yan Xingchun , Liu Huaxing , Li Yajie , Wang Xikun
Published: December 2024

New conceptual designs for floating offshore wind platforms (FOWPs) are crucial for deep-sea wind power generation, increasing power output, lowering construction costs, and minimizing the risk of damage. While there have been various conceptual designs, tailored solutions for the South China Sea are limited due to the relatively harsh environment. This study proposes a novel 10 MW FOWP—“SparFloat”, which combines the advantages of a semi-submersible platform and Spar platform to cater for the sea conditions of the South China Sea. By systematically adjusting the distance between columns and the diameters of the side column and heave plate, the impact of geometrical changes in the platform on its dynamic response is investigated for the purpose of design optimization. The results highlight that roll/pitch natural periods are predominantly governed by restoring stiffness, whereas heave motion exhibits a higher sensitivity to added mass and radiation damping. Increasing the inter-column distance and side column diameter enhances stability and reduces roll/pitch natural periods, while enlarging the heave plate diameter extends the heave natural period. Time-domain simulations using a coupled FAST-AQWA framework confirm that the optimized design meets rule requirements, verifying the robustness and suitability of the SparFloat concept for the challenging environment of the South China Sea.
Production of Vespa tropica Hyaluronidase by Pichia pastoris
Authors: Janpan Piyapon , Schmelzer Bernhard , Klamrak Anuwatchakij , Tastub Patthana , Upathanpreecha Tewa , Rahman Shaikh Shahinur , Nabnueangsap Jaran , Saengkun Yutthakan , Rungsa Prapenpuksiri , Mattanovich Diethard , Daduang Sakda
Published: December 2024

Hyaluronidases have been a subject of great interest in medical and cosmeceutical applications. Previously, our group demonstrated that the venom glands of Vespa tropica contain hyaluronidase enzymes (VesT2s), and heterologous expression of the corresponding gene (VesT2a) in E. coli systems results in inclusion bodies, necessitating functional folding using urea. Here, we report the successful heterologous expression of VesT2a in the Pichia pastoris expression system, with gene construction achieved using GoldenPiCS. After confirming gene integration in the yeast genome, methanol-induced cultures yielded an exceptional amount of VesT2a, approximately two-fold higher than that obtained with the constitutive expression vector (PGAP). Upon culturing in a bioreactor, yeast cells harboring pAOX1-αMF-VesT2a produced secreted proteins with a total yield of 96.45 mg/L. The secreted VesT2a has a molecular weight of 59.35 kDa, significantly higher than the expected molecular weight (~40.05 kDa), presumably due to endogenous glycosylation by the yeast cells. It exhibits optimal activity at 37 °C and pH 3, showing a specific activity of 4238.37 U/mg, and remains active across a broad range of pH and temperature. Notably, it exhibits higher hyaluronidase activity than the crude venom and E. coli-expressed protein, likely due to improved folding via endogenous post-translational modifications, such as disulfide bonds and N-glycosylation; this underscores the potential of heterologous systems for producing venomous hyaluronidases from other species. In silico docking-based analyses further support its catalytic activity and provide insights into seeking natural inhibitors from phenolic-rich plant extracts to alleviate symptoms in patients suffering from insect bites and stings.
Vegetation and Precipitation Patterns Define Annual Dynamics of CO2 Efflux from Soil and Its Components
Authors: Khoroshaev Dmitriy , Kurganova Irina , Lopes de Gerenyu Valentin , Sapronov Dmitry , Kivalov Sergey , Aloufi Abeer S. , Kuzyakov Yakov
Published: December 2024

Respiration of soil heterotrophs—mainly of bacteria and fungi—is a substantial part of carbon balance in terrestrial ecosystems, which tie up organic matter decomposition with the rise of atmospheric CO2 concentration. Deep understanding and prediction of seasonal and interannual variation of heterotrophic and autotrophic components of CO2 efflux from soil is limited by the lack of long-term, full-year measurements. To better understand the impact of current climate changes on CO2 emissions from soils in the mixed forest and mowed grassland, we measured CO2 efflux every week for 2 years. Heterotrophic (SOM-derived + leaf litter) and root-associated (root with rhizosphere microorganisms) components were partitioned by the root exclusion method. The total CO2 efflux from soil was averaged 500 g C m−2 yr−1 in the forest and 650 g C m−2 yr−1 in the grassland, with shares of the no-growing cold season (Nov–Mar) of 22% and 14%, respectively. The heterotrophic component of CO2 efflux from the soil averaged 62% in the forest and 28% in the grassland, and it was generally stable across seasons. The redistribution of the annual precipitation amounts as well as their deficit (droughts) reduced soil respiration by 33–81% and heterotrophic respiration by 24–57% during dry periods. This effect was more pronounced in the grassland (with an average decline of 56% compared to 39% in the forest), which is related to lower soil moisture content in the grassland topsoil during dry periods.
A Cost–Benefit Analysis of Reclaimed Water and Desalinated Seawater for Irrigation in Axarquia, Southern Spain
Authors: Hurtado Antonio R. , Berbel Julio
Published: December 2024

In water-scarce regions, policymakers resort to reclaimed water (RW) and desalinated seawater (DSW) to supply economic agents when conventional resources are not available. This paper develops a proposal for a simple methodology to apply a cost–benefit analysis (CBA) approach to RW and DSW as a support to the evaluation of water policy goals. To test the method, it applies a CBA to RW and DSW in Axarquia (southern Spain), a water-scarce region suffering a long and extreme drought that is impacting urban supply and the agricultural sector. The benefit-to-cost ratio (BCR) of 17.02 estimated for reclaimed water highlights its high return on investment, suggesting that it offers substantial economic and environmental benefits. This high BCR indicates that reclaimed water as a resource for irrigation is highly cost-effective, particularly if it is combined with nutrient management. By contrast, the estimated BCR of 4.05 for desalinated water, although positive, is considerably lower, suggesting that desalinated water is a feasible but less cost-effective solution due to its high energy requirements and associated costs. This methodology may be extended to other hydrological systems, such as aquifer basins, to promote the generation of more comprehensive insights.
Navigating Ecological–Economic Interactions: Spatiotemporal Dynamics and Drivers in the Lower Reaches of the Jinsha River
Authors: Ni Zhongyun , Zhao Yinbing , Liu Jingjing , Li Yongjun , Xia Xiaojiang , Zhang Yang
Published: December 2024

The lower reaches of the Jinsha River, serving as a vital ecological barrier in southwestern China and playing a crucial role in advancing targeted poverty alleviation efforts, remain underexplored in terms of the coupling between ecological and economic development, creating a gap in understanding the region’s sustainable development potential. This study combines the remote sensing ecological index (RSEI) derived from MODIS data and the biodiversity richness index (BRI) based on land use data to create the ecological environment index (EEI) using a weighted approach. It also develops the economic development index (EDI) from economic data using the entropy weight method. By integrating the EEI and EDI, the study calculates key metrics, including the ecological–economic coupling degree (EECD), coupling coordination degree (EECCD), and relative development degree (EERDD), and examines their spatiotemporal changes from 2000 to 2020. Additionally, the study applies a geographic detector model to identify the spatial drivers of the EEI, an obstacle factor diagnosis model to pinpoint the main barriers to EDI, and a neural network model to uncover the underlying forces shaping EECCD. The results indicate that: (I) From 2000 to 2020, the overall improvement rate of the ecological and economic subsystems was greater than that of the ecological–economic coupling system. The entire region is still in the Running-In Stage, and the coordination level has been upgraded from near imbalance to marginal coordination. About 85% of the counties’ EERDDs are still in the EDI Behind EEI Stage. (II) The structural composition of the EEI shows a pattern of low Dry Hot Valley Area and high in other areas, mainly driven by natural factors, although human activities had a notable impact on these interactions. (III) Originating from an impact model primarily driven by economic factors and supplemented by ecological factors, both EDI and EECCD exhibit a pattern of high in the south and low in the north, with improvements spreading northward from the urban area of Kunming. The development gradient differences between 24 poverty-stricken counties and 16 non-poverty-stricken counties have been reduced. (IV) For the six types of ecological–economic coupling development zones, it is essential to adopt localized approaches tailored to the differences in resource and environmental characteristics and development stages. Key efforts should focus on enhancing ecological protection and restoration, increasing financial support, implementing ecological compensation mechanisms, and promoting innovative models for sustainable development.