bluuebunny/crossref_metadata_2025 huggingface dataset:
Metagenomic Analysis of Fecal Archaea, Bacteria, Eukaryota, and Virus in Przewalski's Horses Following Anthelmintic Treatment
Authors: Hu Dini , Yang Jianming , Qi Yingjie , Li Boling , Li Kai , Mok Kai Meng
Published: August 2021

Intestinal microbiota is involved in immune response and metabolism of the host. The frequent use of anthelmintic compounds for parasite expulsion causes disturbance to the equine intestinal microbiota. However, most studies were on the effects of such treatment on the intestinal bacterial microbes; none is on the entire microbial community including archaea and eukaryotic and viral community in equine animals. This study is the first to explore the differences of the microbial community composition and structure in Przewalski's horses prior to and following anthelmintic treatment, and to determine the corresponding changes of their functional attributes based on metagenomic sequencing. Results showed that in archaea, the methanogen of Euryarchaeota was the dominant phylum. Under this phylum, anthelmintic treatment increased the Methanobrevibacter genus and decreased the Methanocorpusculum genus and two other dominant archaea species, Methanocorpusculum labreanum and Methanocorpusculum bavaricum. In bacteria, Firmicutes and Bacteroidetes were the dominant phyla. Anthelmintic treatment increased the genera of Clostridium and Eubacterium and decreased those of Bacteroides and Prevotella and dominant bacteria species. These altered genera were associated with immunity and digestion. In eukaryota, anthelmintic treatment also changed the genera related to digestion and substantially decreased the relative abundances of identified species. In virus, anthelmintic treatment increased the genus of unclassified_d__Viruses and decreased those of unclassified_f__Siphoviridae and unclassified_f__Myoviridae. Most of the identified viral species were classified into phage, which were more sensitive to anthelmintic treatment than other viruses. Furthermore, anthelmintic treatment was found to increase the number of pathogens related to some clinical diseases in horses. The COG and KEGG function analysis showed that the intestinal microbiota of Przewalski's horse mainly participated in the carbohydrate and amino acid metabolism. The anthelmintic treatment did not change their overall function; however, it displaced the population of the functional microbes involved in each function or pathway. These results provide a complete view on the changes caused by anthelmintic treatment in the intestinal microbiota of the Przewalski's horses.
Opening Schools and Trends in SARS-CoV-2 Transmission in European Countries
Authors: Buja Alessandra , Zabeo Federico , Cristofori Vittorio , Paganini Matteo , Baldovin Tatjana , Fusinato Riccardo , Boccuzzo Giovanna , Cocchio Silvia , Coretti Silvia , Rebba Vincenzo , Parpinel Maria
Published: August 2021

Objectives: Benefits of school attendance have been debated against SARS-CoV-2 contagion risks. This study examined the trends of contagion before and after schools reopened across 26 countries in the European Union.

Methods: We compared the average values of estimated Rt before and after school reopening, identifying any significant increase with a one-sample t-test. A meta-analysis and meta-regression analysis were performed to calculate the overall increase in Rt for countries in the EU and to search for relationships between Rt before schools reopened and the average increase in Rt afterward.

Results: The mean reproduction number increased in 16 out of 26 countries. The maximum increase in Rt was reached after a mean 28 days. We found a negative relationship between the Rt before school reopening and its increasing after that event. By 45 days after the first day of school reopening, the overall average increase in Rt for the European Union was 23%.

Conclusion: We observed a significant increase in the mean reproduction number in most European countries, a public health issue that needs strategies to contain the spread of COVID-19.
Pseudomonas putida Isolation and Quantification by Real-Time PCR in Agricultural Soil Biodegradable Mulching
Authors: Fontanazza Stefania , Restuccia Alessia , Mauromicale Giovanni , Scavo Aurelio , Abbate Cristina
Published: August 2021

To reduce the plastic waste problem in agriculture, biodegradable plastic (BP) mulch films have become of key importance thanks to their biodegradability and beneficial effects on crops. However, at present, BPs cannot always replace conventional plastics, because biodegradation is governed by many biotic and abiotic factors under field conditions. This research aimed at isolating and identifying, from soil particles directly attached to the surface of BP samples, the microorganisms responsible of degradation through a combined approach based on biodegradation and molecular tests. For this purpose, a field trial within a Mediterranean apricot orchard was carried out to study the biodegradation of a commercial BP mulch with respect to a no-BP, a conventional apricot management, following the standard agricultural practices, and a subterranean clover cover cropping, either incorporating or leaving its dead mulches on the soil surface. After BP film appeared visibly degraded in field, we isolated from soil particles attached to the polymer surface, a mesophilic bacterium with certain degradative potential assessed by plate and liquid assays, identified by sequencing as Pseudomonas putida. Quantitative real time PCR analysis showed the P. putida was significantly more abundant in PB plots than the other plot treatments. These preliminary results are potentially applicable to accelerate the degradation of BP mulch films and decrease the plastic pollution in agriculture.
Effects of the Harvest Stage of Maize Hybrids on the Chemical Composition of Plant Fractions: An Analysis of the Different Types of Silage
Authors: Horst Egon Henrique , Bumbieris Junior Valter Harry , Neumann Mikael , López Secundino
Published: August 2021

The chemical composition of plant components of three maize hybrids harvested at the beginning of six reproductive stages of maturity was compared. The hybrids evaluated included Maximus VIP3, Defender VIP and Feroz VIP, which were evaluated at each of following stages: R1 (grain formation), R2 (milky grain), R3 (pasty grain), R4 (floury grain), R5 (hard grain) and R6 (ripe grain). The advancement in maturation was linearly related to the crude protein (CP) content of the stem, whole plant, and leaves, and there was a difference among the hybrids. Between R4 and R5 stages, Maximus and Defender presented the highest CP contents for husk (6.58 and 5.42% for Maximus; 5.54 and 5.17% for Defender). The neutral detergent fiber (NDF) of the leaves showed a quadratic relationship with the advancement of maturation but did not differ among the hybrids. For all the hybrids, the NDF content in the husk and cobs increased linearly during the reproductive stages (>77 and 78%, respectively, for the three hybrids in R6). Defender had the lowest NDF content of the cob in R3. The acid detergent lignin contents did not differ among stages in the stems, and showed a linear decrease throughout the whole plant, though the contents did not differ among the hybrids. Due to the differences observed, recommendations for harvest based on the maturity stage for each hybrid should be taken into consideration. There seems to be no important distinction among hybrids for harvesting and use of straw. Despite the reduction in grain yield, an early harvest for earlage or snaplage can provide lower lignin content in husk and cob, as well as higher protein content in the husk, favoring the nutritional value of the vegetative fraction (husk and/or cob).
Genotype × Environment Interactions in Crop Breeding
Authors: Egea-Gilabert Catalina , Pagnotta Mario A. , Tripodi Pasquale
Published: August 2021

In the next decades, the agricultural systems will deal with major challenges linked to the expected population growth, climate changes and necessity of sustainable use of resources able to preserve the environment [...]
Analgesic and Sedative Effects of Epidural Lidocaine-Xylazine in Elective Bilateral Laparoscopic Ovariectomy in Standing Mule Mares
Authors: Vullo Cecilia , Tambella Adolfo Maria , Meligrana Marina , Catone Giuseppe
Published: August 2021

The purpose of this study was to determine the analgesic efficacy and safety of epidural lidocaine-xylazine administration in standing mules undergoing elective bilateral laparoscopic ovariectomy in order to suppress unwanted behaviour. Eight mule mares were sedated with intramuscular 0.05 mg/kg acepromazine followed by 1.3 mg/kg of xylazine and 0.02 mg/kg of butorphanol intravenously. Sedation was maintained by a constant rate infusion of 0.6 mg/kg/h of xylazine. The paralumbar fossae were infiltrated with 30 mL of 2% lidocaine. Epidural anaesthesia was performed at the first intercoccygeal space with 0.2 mg/kg of lidocaine and 0.17 mg/kg of xylazine. After 15 min, bilateral laparoscopic ovariectomy was performed. Heart rate, respiratory rate, rectal temperature, invasive arterial blood pressure, degree of analgesia, sedation and ataxia were evaluated during surgery. The laparoscopic ovariectomy was successfully completed in all animals. Sedation and analgesia were considered satisfactory in six out of the eight mules. In conclusion, caudal epidural block allowed surgery to be easily completed in six out of eight. The animals did not show any signs of discomfort associated with nociception and were mostly calm during the procedures, however additional studies are needed to establish epidural doses of xylazine and lidocaine that result in reliable abdominal pain control in mules for standing ovariectomy.
Chorion Alterations in Eyed-Stage Salmonid Eggs Farmed in La Araucanía, Chile: A Retrospective Study
Authors: Valdebenito Iván , Figueroa Elías , Valdebenito Matías , Paiva Luis
Published: August 2021

The chorion is the primary envelop that protects the fish embryo against mechanical actions, pathogens, and abrupt changes in physical and chemicals conditions of the incubation medium. During embryo development, chorion alterations are not rare, but the occurrence of these is scarcely reported. Increased frequency of chorion alterations can result in increased embryo mortality and thus decreased reproductive performance and losses for fish farms. In this study, we characterize different chorion alterations observed in samples collected over 14 years from 12 salmon and trout farms located in the region of La Araucanía in southern Chile, which sent live eyed-stage embryos (‘eyed-eggs’) for quality analysis to our laboratory. We found soft chorion as the most common alteration observed, being present in the whole 14-year series analyzed in Atlantic Salmon (Salmo salar) and affecting up to 35.0% of the samples examined in a year. This alteration also affected up to 20.0 and 5.7% of Coho Salmon (Oncorhynchus kisutch) and Rainbow Trout (Oncorhynchus mykiss) samples analyzed in a year, respectively. We also found an increase of other chorion alterations, including perforated and white-spotted chorion in Atlantic and Coho Salmon, in the last 8 years. Among the three species, Rainbow Trout exhibited fewer chorion alterations. As the embryonated eggs analyzed here were obtained from broodstocks maintained under standard industrial conditions, these alterations might be linked to changes in environmental conditions affecting the incubation water that need to be further investigated.
Comparison of 12 Different Animal Welfare Labeling Schemes in the Pig Sector
Authors: Heinola Katriina , Kauppinen Tiina , Niemi Jarkko K. , Wallenius Essi , Raussi Satu
Published: August 2021

Animal welfare labeling schemes have been developed to respond to consumers’ expectations regarding farm animal welfare. They are designed to certify that labeled products comply with certain animal welfare standards. In this study, 12 pig welfare labeling schemes were reviewed, and their criteria related to pig welfare were compared. Information regarding farrowing criteria, space allowance, outdoor access, mutilations, and provision of enrichments and bedding material were gathered from the labels’ internet pages and documentation. The results indicated a substantial variation between the labels in terms of the level of animal welfare they ensure. While certain schemes barely exceeded the minimum standards for the protection of pigs in the European Union, more demanding tiers of the multitier schemes had the potential to improve animal welfare substantially. The most ambitious tiers of multistage schemes were often comparable to organic standards providing outdoor facilities and additional space. The heterogeneity of the labels’ standards complicates the comparison of labels.
Comparison of Gut Microbiota of 96 Healthy Dogs by Individual Traits: Breed, Age, and Body Condition Score
Authors: You Inhwan , Kim Min Jung
Published: August 2021

Since dogs are part of many peoples’ lives, research and industry related to their health and longevity are becoming a rising topic. Although gut microbiota (GM) is a key contributor to host health, limited information is available for canines. Therefore, this study characterized GM according to individual signatures (e.g., breed, age, and body condition score—BCS) of dogs living in the same environment. Fresh fecal samples from 96 healthy dogs were analyzed by sequencing the V3-V4 region of the 16S rRNA gene. The major microbial phyla were Firmicutes, Bacteroidetes, Fusobacteria, Proteobacteria, and Actinobacteria. In the comparison by breeds, relative abundance of Fusobacterium was significantly differed. Interestingly, Fusobacterium perfoetens abundance was positively correlated with age (p = 0.018), being significantly more enriched in the 6–10-year-old group (14.3%) than in the 0.5–1-year-old group (7.2%). Moreover, despite the healthy appearance of dogs in all age (0.5–10 years) and BCS (3–6) groups, the gut microbial environment may be disadvantageous in older dogs or in dogs with an abnormal BCS. These findings broaden our understanding of gut microbial ecology according to individual characteristics of dogs and may be used as a reference for providing customized-care to companion animals.
Effects of Nutritional Restriction during Laying Period of Fat and Lean Line Broiler Breeder Hens on Meat Quality Traits of Offspring
Authors: Li Feng , Xie Yingjie , Yang Xue , Zhang Yuanyuan , Cheng Baojing , Shan Anshan
Published: August 2021

The offspring meat quality of hens undergoing a 25% dietary restriction treatment during the laying period were evaluated in fat and lean line breeder. A total of 768 female birds (384/line) were randomly assigned to four groups (12 replicates/group, 16 birds/replicates). Maternal feed restriction (MFR) and normal started at 27 weeks of age. Offspring broilers were fed ad libitum. The offspring meat quality traits and muscle fiber morphology in different periods were measured. At birth, significant interactions were found on breast muscle fiber morphology (p < 0.05). At 28 days, MFR decreased breast water content and increased thigh crude fat content, and significant interactions were observed on breast crude fat and protein contents (p < 0.05). At 56 days, MFR affected morphology of peroneus longus muscle tissue, and significant interactions were found on thigh redness at 48 h and amino acid contents in breast and thigh muscle (p < 0.05). Overall, MRF may lead to offspring birth sarcopenia. Such offspring grow more easily to deposit fat in a nutritious environment, but they will self-regulate adverse symptoms during growth and development. The two lines respond differently to maternal nutritional disturbance due to different nutritional requirements and metabolic patterns.
Antimicrobial Resistance in Loggerhead Sea Turtles (Caretta caretta): A Comparison between Clinical and Commensal Bacterial Isolates
Authors: Trotta Adriana , Marinaro Mariarosaria , Sposato Alessio , Galgano Michela , Ciccarelli Stefano , Paci Serena , Corrente Marialaura
Published: August 2021

Gram negative organisms are frequently isolated from Caretta caretta turtles, which can act as reservoir species for resistant microorganisms in the aquatic environment. C. caretta, which have no history of treatment with antimicrobials, are useful sentinel species for resistant microbes. In this culture-based study, commensal bacteria isolated from oral and cloacal samples of 98 healthy C. caretta were compared to clinical isolates from the wounds of 102 injured animals, in order to investigate the presence of AMR bacteria in free-living loggerheads from the Adriatic Sea. A total of 410 isolates were cultured. Escherichia coli and genera such as Serratia, Moraxella, Kluyvera, Salmonella were isolated only in healthy animals, while Acinetobacter, Enterobacter, Klebsiella and Morganella were isolated only from the wounds of the injured animals. When tested for susceptibility to ampicillin, amoxicillin + clavulanic acid, ceftazidime, cefuroxime, gentamicin, doxycycline, ciprofloxacin and enrofloxacin, the clinical isolates showed highly significant differences in AMR rates vs. commensal isolates for all the drugs tested, except for doxycycline. The detection of high AMR rates in loggerheads is of clinical and microbiological significance since it impacts both the choice of a proper antibiotic therapy and the implementation of conservation programs.
Exploratory Analysis of Color Forms’ Variability in the Invasive Asian Lady Beetle Harmonia axyridis (Pallas 1773)
Authors: Lemic Darija , Pajač Živković Ivana , Šuliček Matea , Benítez Hugo A.
Published: August 2021

The Asian ladybird (Harmonia axyridis Pallas), native to Asia, is one of the 100 most invasive species in the world and has spread worldwide. This study aimed to characterize color forms of H. axyridis in Croatia and to analyze the variability of wing shape between populations and indicated forms. Geometric morphometric methods were used to analyze a total of 129 left and right wings in males and 126 left and right wings in females of H. axyridis collected from four different sites in Croatia. The results show a significant difference in wing shapes between the studied forms. Each form had its own specific morphotype that likely originated under the influence of genetic changes in the species. This study demonstrates that the use of geometric morphometric analysis is effective in studying the variability in H. axyridis populations. As this study is the first of its kind, for further clarity, it is necessary to conduct additional studies on a larger number of sites and an equal number of individuals of all forms.
Socioeconomic Factors Contributing to Antibiotic Resistance in China: A Panel Data Analysis
Authors: Zhen Xuemei , Chen Jingchunyu , Sun Xueshan , Sun Qiang , Guo Shasha , Stålsby Lundborg Cecilia
Published: August 2021

The relationship between socioeconomic factors and antibiotic resistance (ABR) prevalence remains a knowledge gap in China. In this study, our aim was to examine the association between ABR prevalence and socioeconomic factors across 30 provinces in mainland China. We used two measures of level of ABR: the proportion of methicillin-resistant Staphylococcus aureus (MRSA), third-generation cephalosporin-resistant Escherichia coli (3GCREC), and third-generation cephalosporin-resistant Klebsiella pneumoniae (3GCRKP), and the aggregate resistance. The data of ABR prevalence, education, gross domestic product (GDP) per capita, out-of-pocket (OOP) health expenditure, physician density, hospital bed density, and public toilet density during 2014 and 2018 in 30 provinces in mainland China were included. We examined the association between ABR prevalence and potential contributing socioeconomic factors using panel data modeling. In addition, we explored this relationship in the eastern, central, and western economic zones. Our results indicated that GDP per capita was significantly positively correlated with ABR in mainland China and the eastern economic zone; however, significantly positive associations did not exist in the central and western economic zones. Surprisingly, both higher GDP per capita and higher OOP health expenditure were associated with a higher level of MRSA, but a lower level of 3GCREC; higher physician density was associated with a lower level of MRSA, but a higher level of 3GCREC. In addition, ABR prevalence presented a decline trend during 2014 and 2018. Our study showed the potential associ-ations between resistance and GDP per capita, OOP health expenditure, physician density. It high-lights that the social and economic determinants can be of importance in tacking the development and spread of ABR in mainland China.
Antimicrobial Stewardship Activities in Public Healthcare Facilities in South Africa: A Baseline for Future Direction
Authors: Engler Deirdré , Meyer Johanna Catharina , Schellack Natalie , Kurdi Amanj , Godman Brian
Published: August 2021

Antimicrobial resistance (AMR) is a growing problem worldwide, including South Africa, where an AMR National Strategy Framework was implemented to instigate antimicrobial stewardship programmes (ASPs) and improve antimicrobial prescribing across sectors. To address the need to assess progress, a sequential mixed methodology with an explanatory research design was employed. In Phase 1, a self-administered questionnaire was completed by healthcare professionals (HCPs) from 26 public sector healthcare facilities across South Africa to assess compliance with the Framework. The results were explored in Phase 2 through 10 focus group discussions and two in-depth interviews, including 83 participants. Emerging themes indicated that public healthcare facilities across South Africa are facing many challenges, especially at entry level primary healthcare (PHC) facilities, where antimicrobial stewardship activities and ASPs are not yet fully implemented. Improved diagnostics and surveillance data are a major shortcoming at these facilities. Continuous education for HCPs is deficient, especially for the majority of prescribers at PHC level and health campaigns are nearly non-existent. Involvement and visibility of management at certain facilities is a serious shortfall. Consequently, it is important to call attention to the challenges faced with improving antimicrobial prescribing across countries and address these to reduce AMR, especially in PHC facilities, being the first point of access to healthcare for the vast majority of patients in developing countries.
Evidence-Based Tracking of MDR E. coli from Bovine Endometritis and Its Elimination by Effective Novel Therapeutics
Authors: Shafique Laiba , Wu Siwen , Aqib Amjad Islam , Ali Muhammad Muddassir , Ijaz Misbah , Naseer Muhammad Aamir , Sarwar Zaeem , Ahmed Rais , Saleem Arslan , Qudratullah  , Ahmad Abdullah Saghir , Pan Hongping , Liu Qingyou
Published: August 2021

Antibiotic-resistant bacteria have become the predominant etiology of endometritis and thus require effective treatment approaches. We used ultrasonography coupled with clinical signs and presented complaints of reproductive issues to investigate the epidemiology, phylogenetic analysis, antimicrobial resistance, and development of novel therapeutics against Escherichia coli isolated from endometritis in bovine (n = 304 from 10 commercial dairy farms). The prevalence of bovine endometritis in this study was 43.75%, while among these, 72.18% samples were positive for E. coli. Nucleotide analysis performed through BLAST and MEGAX showed 98% similarity to the nucleotide sequence of the reference E. coli strain (accession number CP067311.1). The disk diffusion assay revealed pathogen resistance to most antibiotics. Pattern of MIC order of resistance was as follows: enrofloxacin < gentamicin < co-amoxiclav < streptomycin < amoxicillin < metronidazole < oxytetracycline. Field trials revealed the highest recovery rate (in terms of clearance of endometritis and establishment of pregnancy) in case of gentamicin + enrofloxacin (100%) and gentamicin alone (100%), followed by co-amoxiclav + gentamicin (84.61%), oxytetracycline alone (78.57%), and metronidazole + enrofloxacin (33.33%). Hence, the current study reported a higher prevalence of multidrug-resistant E. coli showing considerable similarity with reference strain, and finally, the effective response of novel antibiotics to treat cases.
Effects of Water Deficit Irrigation on Phenolic Composition and Antioxidant Activity of Monastrell Grapes under Semiarid Conditions
Authors: Pérez-Álvarez Eva P. , Intrigliolo Diego S. , Almajano María Pilar , Rubio-Bretón Pilar , Garde-Cerdán Teresa
Published: August 2021

The high phenolic compound content of grapes makes them an important source of natural antioxidants, among other beneficial health properties. Vineyard irrigation might affect berry composition and quality. Regulated deficit irrigation (RDI) is a widely used strategy to reduce the possible negative impact of irrigation on grapes, improving grape composition and resulting in water savings. Monastrell grapevines (Vitis vinifera L.) grown in eastern Spain were subjected to two water regime strategies: rainfed (non-irrigation) and RDI. The content of anthocyanins, flavonols, flavanols, hydroxybenzoic and hydroxycinnamic acids, and stilbenes was determined by HPLC and was related with total phenolic content and three antioxidant activity methods (ABTS, DPPH, and ORAC). The study aimed to evaluate and compare the phenolic composition and antioxidant potential of Monastrell grapes. The rainfed regime concentrated grapes in terms of phenolic compounds. Thus, total content of anthocyanins, flavonols, flavanols, hydroxybenzoic acids, and total phenols were higher in the rainfed grapes than in the RDI ones. Besides, the rainfed grapes doubled their antioxidant potential with respect to the RDI grapes with the ORAC method. Total phenolic content and antioxidant activity by ORAC assay positively correlated with most of the total phenolic compounds analyzed. This study demonstrates how field practices can modulate final grape composition in relation to their antioxidant activity.
Theoretical Study of Radical Inactivation, LOX Inhibition, and Iron Chelation: The Role of Ferulic Acid in Skin Protection against UVA Induced Oxidative Stress
Authors: Amić Ana , Dimitrić Marković Jasmina M. , Marković Zoran , Milenković Dejan , Milanović Žiko , Antonijević Marko , Mastiľák Cagardová Denisa , Rodríguez-Guerra Pedregal Jaime
Published: August 2021

Ferulic acid (FA) is used in skin formulations for protection against the damaging actions of the reactive oxygen species (ROS) produced by UVA radiation. Possible underlying protective mechanisms are not fully elucidated. By considering the kinetics of proton-coupled electron transfer (PCET) and radical-radical coupling (RRC) mechanisms, it appears that direct scavenging could be operative, providing that a high local concentration of FA is present at the place of •OH generation. The resulting FA phenoxyl radical, after the scavenging of a second •OH and keto-enol tautomerization of the intermediate, produces 5-hydroxyferulic acid (5OHFA). Inhibition of the lipoxygenase (LOX) enzyme, one of the enzymes that catalyse free radical production, by FA and 5OHFA were analysed. Results of molecular docking calculations indicate favourable binding interactions of FA and 5OHFA with the LOX active site. The exergonicity of chelation reactions of the catalytic Fe2+ ion with FA and 5OHFA indicate the potency of these chelators to prevent the formation of •OH radicals via Fenton-like reactions. The inhibition of the prooxidant LOX enzyme could be more relevant mechanism of skin protection against UVA induced oxidative stress than iron chelation and assumed direct scavenging of ROS.
Early Cytokine-Induced Transient NOX2 Activity Is ER Stress-Dependent and Impacts β-Cell Function and Survival
Authors: Vilas-Boas Eloisa A. , Carlein Christopher , Nalbach Lisa , Almeida Davidson C. , Ampofo Emmanuel , Carpinelli Angelo R. , Roma Leticia P. , Ortis Fernanda
Published: August 2021

In type 1 diabetes (T1D) development, proinflammatory cytokines (PIC) released by immune cells lead to increased reactive oxygen species (ROS) production in β-cells. Nonetheless, the temporality of the events triggered and the role of different ROS sources remain unclear. Isolated islets from C57BL/6J wild-type (WT), NOX1 KO and NOX2 KO mice were exposed to a PIC combination. We show that cytokines increase O2•− production after 2 h in WT and NOX1 KO but not in NOX2 KO islets. Using transgenic mice constitutively expressing a genetically encoded compartment specific H2O2 sensor, we show, for the first time, a transient increase of cytosolic/nuclear H2O2 in islet cells between 4 and 5 h during cytokine exposure. The H2O2 increase coincides with the intracellular NAD(P)H decrease and is absent in NOX2 KO islets. NOX2 KO confers better glucose tolerance and protects against cytokine-induced islet secretory dysfunction and death. However, NOX2 absence does not counteract the cytokine effects in ER Ca2+ depletion, Store-Operated Calcium Entry (SOCE) increase and ER stress. Instead, the activation of ER stress precedes H2O2 production. As early NOX2-driven ROS production impacts β-cells’ function and survival during insulitis, NOX2 might be a potential target for designing therapies against early β-cell dysfunction in the context of T1D onset.
Assessment of Manufacturing Parameters for New 3D-Printed Heating Circuits Based on CNT-Doped Nanocomposites Processed by UV-Assisted Direct Write
Authors: Cortés Alejandro , Jiménez-Suárez Alberto , Campo Mónica , Ureña Alejandro , Prolongo Silvia G.
Published: August 2021

This work consists of the development of an easy strategy to transform any structure into an efficient surface heater by the application of a low voltage over 3D printed nanocomposite circuits. To this end, the electrical conductivity and self-heating capabilities of UV-Assisted Direct Write 3D printed circuits doped with carbon nanotubes were widely explored as a function of the number of printed layers. Moreover, an optimization of the printing process was carried out by comparing the accuracy and printability obtained when printing with two different configurations: extruding and curing the ink in the same stage or curing the extruded ink in a second stage, after the whole layer was deposited. In this regard, the great homogeneity and repeatability of the heating showed by the four-layer printed circuits, together with their excellent performance for long heating times, proved their applicability to convert any structure to a surface heater. Finally, the deicing capability of the four-layer circuit was demonstrated, being able to remove a 2.5 mm thick ice layer in 4 min and 4 s.
Characteristics of Restrained Drying Shrinkage on Arched Steel Fiber-Reinforced Concrete
Authors: Kim Dae-Jin , Kim Sun-Hee , Choi Won-Chang
Published: August 2021

The volumetric changes of concrete, including drying shrinkage, are effectively controlled in steel fiber-reinforced concrete (SFRC) mixtures due to the action of the included steel fiber. The current code provision in ANSI/SDI C-2017 allows a minimum steel fiber content of 0.2% of the volume fraction of concrete to control drying shrinkage and to manage cracking in the slab. Limited research has addressed replacing the shear reinforcement in concrete beams with steel fiber. In this study, we used newly developed arched steel fiber to evaluate shrinkage characteristics, including free-drying shrinkage and restrained drying shrinkage, of SFRC and scaled-down deck slab elements. We compared the measured drying shrinkage test results to predicted results obtained from models found in the literature. We confirmed that, overall, the number, width, and length of cracks were reduced significantly at the surface of SFRC slabs when arched steel fiber at 0.2% volume fraction was included in the mixture.
Deep Learning Based Android Anomaly Detection Using a Combination of Vulnerabilities Dataset
Authors: Namrud Zakeya , Kpodjedo Sègla , Talhi Chamseddine , Bali Ahmed , Boaye Belle Alvine
Published: August 2021

As the leading mobile phone operating system, Android is an attractive target for malicious applications trying to exploit the system’s security vulnerabilities. Although several approaches have been proposed in the research literature for the detection of Android malwares, many of them suffer from issues such as small training datasets, there are few features (most studies are limited to permissions) that ultimately affect their performance. In order to address these issues, we propose an approach combining advanced machine learning techniques and Android vulnerabilities taken from the AndroVul dataset, which contains a novel combination of features for three different vulnerability levels, including dangerous permissions, code smells, and AndroBugs vulnerabilities. Our approach relies on that dataset to train Deep Learning (DL) and Support Vector Machine (SVM) models for the detection of Android malware. Our results show that both models are capable of detecting malware encoded in Android APK files with about 99% accuracy, which is better than the current state-of-the-art approaches.
A Synthesized Study Based on Machine Learning Approaches for Rapid Classifying Earthquake Damage Grades to RC Buildings
Authors: Harirchian Ehsan , Kumari Vandana , Jadhav Kirti , Rasulzade Shahla , Lahmer Tom , Raj Das Rohan
Published: August 2021

A vast number of existing buildings were constructed before the development and enforcement of seismic design codes, which run into the risk of being severely damaged under the action of seismic excitations. This poses not only a threat to the life of people but also affects the socio-economic stability in the affected area. Therefore, it is necessary to assess such buildings’ present vulnerability to make an educated decision regarding risk mitigation by seismic strengthening techniques such as retrofitting. However, it is economically and timely manner not feasible to inspect, repair, and augment every old building on an urban scale. As a result, a reliable rapid screening methods, namely Rapid Visual Screening (RVS), have garnered increasing interest among researchers and decision-makers alike. In this study, the effectiveness of five different Machine Learning (ML) techniques in vulnerability prediction applications have been investigated. The damage data of four different earthquakes from Ecuador, Haiti, Nepal, and South Korea, have been utilized to train and test the developed models. Eight performance modifiers have been implemented as variables with a supervised ML. The investigations on this paper illustrate that the assessed vulnerability classes by ML techniques were very close to the actual damage levels observed in the buildings.
Development of Defect Detection AI Model for Wire + Arc Additive Manufacturing Using High Dynamic Range Images
Authors: Lee Chaekyo , Seo Gijeong , Kim Duck Bong , Kim Minjae , Shin Jong-Ho
Published: August 2021

Wire + arc additive manufacturing (WAAM) utilizes a welding arc as a heat source and a metal wire as a feedstock. In recent years, WAAM has attracted significant attention in the manufacturing industry owing to its advantages: (1) high deposition rate, (2) low system setup cost, (3) wide diversity of wire materials, and (4) sustainability for constructing large-sized metal structures. However, owing to the complexity of arc welding in WAAM, more research efforts are required to improve its process repeatability and advance part qualification. This study proposes a methodology to detect defects of the arch welding process in WAAM using images acquired by a high dynamic range camera. The gathered images are preprocessed to emphasize features and used for an artificial intelligence model to classify normal and abnormal statuses of arc welding in WAAM. Owing to the shortage of image datasets for defects, transfer learning technology is adopted. In addition, to understand and check the basis of the model’s feature learning, a gradient-weighted class activation mapping algorithm is applied to select a model that has the correct judgment criteria. Experimental results show that the detection accuracy of the metal transfer region-of-interest (RoI) reached 99%, whereas that of the weld-pool and bead RoI was 96%.
Non-Melanoma Facial Skin Tumors—The Correspondence between Clinical and Histological Diagnosis
Authors: Tamas Camelia , Hreniuc Irina Mihaela Jemnoschi , Tecuceanu Angela , Ciuntu Bogdan Mihnea , Ibanescu Clara Larisa , Tamas Ioana , Ianole Victor , Stanescu Cristina , Pintilie Catalina Teodora , Zamfir Carmen Lacramioara , Moraru Dan Cristian
Published: August 2021

This paper consists of a retrospective study that includes a total of 186 cases with facial skin tumors diagnosed and treated between January 2015 and December 2017. Our aim was to correlate the initial clinical diagnosis with the histological results, in order to observe the success rate of our clinical experience, but also to underline that certain clinical tumor aspects can be misleading as the histological findings can result in different final diagnostics. From the total number of cases, we counted a total of 226 tumors (both benign and malignant), from which 194 had the clinical diagnosis confirmed by the anatomopathological exam and 34 had a different laboratory result from the clinical presumption: 10 benign tumors and 22 malignant tumors (eight BCC and 14 SCC, 12 of the latter being confused with ulcerovegetant BCC). Detailed correlation data were presented, as well as different situations of incongruence between the clinical and the histological diagnosis. The long-term goal of the study was to use our experience of correlating the clinical and the histological diagnosis, in order to improve the existing knowledge on the clinical differential diagnosis of both malignant and benign facial skin tumors.
Seasonal Population Structure of the Copepod Temora turbinata (Dana, 1849) in the Kuroshio Current Edge, Southeastern East China Sea
Authors: Wang Yan-Guo , Tseng Li-Chun , Xing Bing-Peng , Sun Rou-Xin , Chen Xiao-Yin , Wang Chun-Guang , Hwang Jiang-Shiou
Published: August 2021

The abundance of adult males, females, and copepodites, and sex ratio of a Temora turbinata population and seawater hydrology were studied from 2018 to 2019 in waters off northeast Taiwan, northwest Pacific Ocean. The hydrological parameters showed significant differences between sampling months caused by interactions of Kuroshio, East China Sea water, and the China Coastal Current. The highest average abundance was recorded from the June 2018 cruise with 2903.92 ± 3499.47 (inds. m−3), followed by a cruise in June 2019 with an average abundance of 1990.64 ± 1401.55 (inds. m−3). The sex ratio ranged between 0.25 and 2.33; the records were significantly higher in samples of June 2018 than during other sampling cruises (one-way ANOVA). The spatiotemporal distribution of T. turbinata showed a clear pattern of seasonal changes among sampling stations and distribution zones. Abundance of females and copepodites correlated significantly positive (r = 0.755, p < 0.001), whereas sex ratio (r = 0.247, p = 0.119) did not correlate significantly. The present study revealed that the abundance of T. turbinate was highest in June and was positively correlated with seawater temperature; furthermore, this is the first time the in situ sex ratio of T. turbinata in western Pacific waters has been reported.
A Simple Contrast Matching Rule for OSEM Reconstructed PET Images with Different Time of Flight Resolution
Authors: Presotto Luca , Bettinardi Valentino , De Bernardi Elisabetta
Published: August 2021

Background: Time-of-Flight (TOF) is a leading technological development of Positron Emission Tomography (PET) scanners. It reduces noise at the Maximum-Likelihood solution, depending on the coincidence–timing–resolution (CTR). However, in clinical applications, it is still not clear how to best exploit TOF information, as early stopped reconstructions are generally used. Methods: A contrast-recovery (CR) matching rule for systems with different CTRs and non-TOF systems is theoretically derived and validated using (1) digital simulations of objects with different contrasts and background diameters, (2) realistic phantoms of different sizes acquired on two scanners with different CTRs. Results: With TOF, the CR matching rule prescribes modifying the iterations number by the CTRs ratio. Without TOF, the number of iterations depends on the background dimension. CR matching was confirmed by simulated and experimental data. With TOF, image noise followed the square root of the CTR when the rule was applied on simulated data, while a significant reduction was obtained on phantom data. Without TOF, preserving the CR on larger objects significantly increased the noise. Conclusions: TOF makes PET reconstructions less dependent on background dimensions, thus, improving the quantification robustness. Better CTRs allows performing fewer updates, thus, maintaining accuracy while minimizing noise.
Rationale for the Combined Use of Biological Processes and AOPs in Wastewater Treatment Tasks
Authors: Shtepa Vladimir , Balintova Magdalena , Chernysh Yelizaveta , Chubur Viktoriia , Demcak Stefan , Gautier Mathieu
Published: August 2021

This paper aims to form a unified concept of the integrated use of different wastewater treatment methods to form a resistant biological treatment stage of technological systems under the influence of such toxic factors as antibiotics and surfactants. The processes of mechanical treatment, ozonation, UV irradiation, and electrolytic anodic oxidation were implemented in an electrotechnological wastewater treatment facility. Wastewater treatment quality was determined by the concentration of nitrogen compounds in aqueous solutions according to the method of Lurie. Biodiagnostics of the investigated activated sludge via surfactant action was carried out at polyethylene oxide concentrations of 10, 30, and 50 mg/dm3. As a result of experiments on wastewater treatment after aquaculture, an improvement in the reduction of pollutants only by the indicator “nitrate concentration” was determined: by 20% after anodic oxidation, and by 15% after photolysis. At almost all surfactant concentrations studied, the activated sludge was not completely recovered, which was expressed in a decrease in its quantity and in the inability to aggregate flakes of activated sludge. The diameter of the growth retardation of the standard disk with antibiotic (amoxiclav) by the accumulative culture of activated sludge was 17.3 ± 2 mm at a concentration of 4 mg/dm3 and 31.3 ± 3 mm at a concentration of 6 mg/dm3. In the process of studying the state of the activated sludge’s biocenosis under the influence of such toxicants, several regularities were revealed. The directions of using combined approaches of water treatment and wastewater treatment were defined. The structural model of treatment facilities using aerobic and anaerobic bioprocesses together with advanced oxidative technologies was substantiated.
A Review of Self-Coherent Optical Transceivers: Fundamental Issues, Recent Advances, and Research Directions
Authors: Alimi Isiaka , Patel Romil , Silva Nuno , Sun Chuanbowen , Ji Honglin , Shieh William , Pinto Armando , Muga Nelson
Published: August 2021

This paper reviews recent progress on different high-speed optical short- and medium-reach transmission systems. Furthermore, a comprehensive tutorial on high-performance, low-cost, and advanced optical transceiver (TRx) paradigms is presented. In this context, recent advances in high-performance digital signal processing algorithms and innovative optoelectronic components are extensively discussed. Moreover, based on the growing increase in the dynamic environment and the heterogeneous nature of different applications and services to be supported by the systems, we discuss the reconfigurable and sliceable TRxs that can be employed. The associated technical challenges of various system algorithms are reviewed, and we proffer viable solutions to address them.
Appendicular Skeletal Muscle Mass Index and Physiological Performance in Post-Menopausal Women with Total Thyroidectomy
Authors: Wen Wei-Lun , Lin Hsiu-Chu , Yu Hui-Chen , Chen Yi-Pen , Liang Ching-Chao , Hsu Wei-Hao , Hsu Jui-Sheng , Shih Ming-Chen , Lee Mei-Yueh , Chen Szu-Chia
Published: August 2021

Sarcopenia is prevalent in postmenopausal women but is inconclusive in total thyroidectomy and under levothyroxine replacement. We aim to analyze the determinants of sarcopenia and investigate the early detection of sarcopenia in this group. Fifty postmenopausal women with total thyroidectomy were measured for body composition via Dual-energy X-ray Absorptiometry (DXA) and Appendicular Skeletal Muscle mass divided by the height square (ASM/ht2). Handgrip strength and gait speed and Geriatric Nutritional Risk Index (GNRI) were calculated. Eight determinants associated with sarcopenia include GNRI (β, 0.042; 95% confidence interval (CI), 0.021 to 0.064), femoral neck BMD (β, 0.989; 95% CI, 0.049 to 1.929), TSH (β, 0.192; 95% CI, 0.027 to 0.357), and thyroglobulin Ab (0.657; 95% CI, 0.210 to 1.103) for ASM/height2; menopausal years (β, −3.112; 95% CI, −5.661 to −0.563) and ASM/height2 (β, 2.669; 95% CI, 1.073 to 4.265) for handgrip strength; and GNRI (β, 0.062; 95% CI, 0.019 to 0.105), T3 (β, −3.541; 95% CI, −7.019 to −0.063), and age (β, 0.043; 95% CI, 0.003 to 0.084) for gait speed. Our study confirmed a high prevalence of low skeletal muscle mass index in postmenopausal women with total thyroidectomy and revealed a number of determinants that could help early diagnosis and management this disease in daily clinical practice.
Design for Additive Manufacturing and for Machining in the Automotive Field
Authors: Bassoli Elena , Defanti Silvio , Tognoli Emanuele , Vincenzi Nicolò , Degli Esposti Lorenzo
Published: August 2021

High cost, unpredictable defects and out-of-tolerance rejections in final parts are preventing the complete deployment of Laser-based Powder Bed Fusion (LPBF) on an industrial scale. Repeatability, speed and right-first-time manufacturing require synergistic design approaches. In addition, post-build finishing operations of LPBF parts are the object of increasing attention to avoid the risk of bottlenecks in the machining step. An aluminum component for automotive application was redesigned through topology optimization and Design for Additive Manufacturing. Simulation of the build process allowed to choose the orientation and the support location for potential lowest deformation and residual stresses. Design for Finishing was adopted in order to facilitate the machining operations after additive construction. The optical dimensional check proved a good correspondence with the tolerances predicted by process simulation and confirmed part acceptability. A cost and time comparison versus CNC alone attested to the convenience of LPBF unless single parts had to be produced.