bluuebunny/crossref_metadata_2025 huggingface dataset:
A case report of vaginal fibromyoma in a bitch
Authors: Aksu Rabia Buse , Gezer Bahar , Özbezek Mehmet Fatih , Küçükbekir Çağla Nur , Kırşan İsmail , Yılmaz Merve , Baykal Aslihan , Günay Uçmak Zeynep , Haktanır Damla , Yıldırım Funda , Kekeç Ilgın , Halaç Barış
Published: June 2024

A 5-year-old, intact female Caucasian Shepherd dog was brought to our clinic with complaints of ongoing vaginal bleeding, weakness, loss of appetite, and a vaginal mass for a month. According to the anamnesis, the vaginal mass had rapidly increased in size over the last month, and there was ulceration and bleeding on the mass. In the examination, it was determined that the mass originated from the ventrolateral surface of the vagina, and an abscess-like structure was also found cranially to this mass. The mucous membranes were light pink, the palpable lymph nodes were of average size, the furs were matte, and the vaginal area was dirty with blood. The dog’s hemogram and biochemical test results were found to be within normal reference ranges; hence, it was decided to treat the dog surgically. The tissue obtained after vaginal mass excision was examined microbiologically and histopathologically. The mass, initially thought to be an abscess based on its smaller size and macroscopic examination, was determined to have a sterile content and to be an inflammatory infiltration rather than an abscess. It was concluded that detailed clinical examination and histopathological analysis can establish the diagnosis of all tumoral cases like this, and successful treatment can be achieved through surgical intervention.
Persistence of phorate and its Residual Toxicity to Aphis Craccivora Koch. in Cowpea
Authors: Visalakshi A. , Nair M.R.G.K. , Jacob Abraham
Published: June 2024

Enzymatic assay of internal residues of phorate in cowpea yigiia sinensis  following application of the insecticide as granules with seed at 1, 2 and 3 kg a.i. / ha showed that residues of the toxicant persisted within the plants upto 49 days following the application. On the 56th day, there was no residue of the insecticide either in the leaves or in the pods. The mortality of Aphis craccivora exposed to the plants treated with the granules was 100 percent upto 14 days with I and 2 kg a.i / ha and upto 21 days with 3 kg a.i/ha. The mortality showed a gradual reduction corresponding to the reduction of the internal residue of the insecticide, there being no mortality on on 56th day of insecticide application. Application of phorate granules at 2kga.i/ha, with the seeds is considered an effective and safe method for protecting Cowpea from infestation bv A. craccivora up to 8 weeks of its growth.
Penerapan Nilai Konservasi Tinggi (NKT) pada Perkebunan Kelapa Sawit di Provinsi Riau
Authors: Nurjannah Siti , Masyithoh Galuh , Sunkar Arzyana , Zuhud Ervizal
Published: June 2024

Perkebunan kelapa sawit menjadi komoditas unggulan di Indonesia. Hal ini menghasilkan luasan perkebunan kelapa sawit semakin meningkat. Perkebunan kelapa sawit di Indonesia diwajibkan melakukan sertifikasi ISPO dan RSPO untuk pembangunan perkebunan kelapa sawit secara berkelanjutan. Salah satu prinsip yang harus dilakukan yaitu pengelolaan lingkungan hidup,  sumberdaya alam, dan keanekaragaman hayati pada kawasan lindung atau areal konservasi tinggi (NKT). NKT merupakan salah satu instrument penilaian sertifikasi ISPO maupun RSPO. Tujuan penelitian yaitu untuk menganalisis penerapan NKT dalam perkebunan kelapa sawit. Penelitian dilakukan pada 7 perusahaan kelapa sawit Provinsi Riau dengan metode wawancara terpadu, analisis dokumen, dan melihat langsung di lapangan dengan melakukan inventarisasi potensi NKT. Hasil penelitian menunjukkan bahwa pada tahun 2016, 6 dari 7 perusahaan telah melakukan kajian NKT, sedangkan satu diantaranya dilakukan penilaian NKT pada tahun 2017. Jenis NKT yang ditemukan yaitu NKT 1.2, NKT 1.3, NKT 4.1, NKT 4.2, NKT 4.3, NKT 5, dan NKT 6. Hasil pengamatan di lapangan sudah terdapat papan informasi pada beberapa NKT dan pengayaan jenis tumbuhan. Jenis NKT pada dokumen dan inventarisasi di lapangan menunjukkan hasil yang hampir sepadan. Hasil inventarisasi mamalia dan burung terdapat perbedaan karena pengambilan data pada tahun yang berbeda. Terdapat beberapa NKT 4 (sempadan sungai) masih ditanami kelapa sawit. Pengelolaan yang dilakukan pada areal sempadan sungai yaitu tidak dilakukan penanaman kembali kelapa sawit, pengayaan jenis tumbuhan lokal, dan tidak menggunakan pupuk kimia.
Keanekaragaman Jenis Kupu-Kupu (Rhopalocera) Di Sungai Oba Kecamatan Oba Utara Kota Tidore KepulauanKeanekaragaman Jenis Kupu-Kupu (Rhopalocera) Di Sungai Oba Kecamatan Oba Utara Kota Tidore Kepulauan
Authors: Tukuboya Elvira , Kurniawan Andy , Fatrawana Adesna
Published: June 2024

Kupu-kupu adalah bagian dari ekosistem yang fungsinya mempertahankan keseimbangan ekosistem dan memperkaya keanekaragaman hayati. Tujuan penelitian ini yaitu untuk mengidentifikasi habitat kupu-kupu dan menganalisis keanekaragaman jenis kupu-kupu di Sungai Oba. Metode yang digunakan dalam penelitian ini yaitu observasi atau pengamatan langsung di lapangan. Identifikasi habitat dengan mengamati vegetasi, suhu, dan kelembapan. Pengumpulan data kupu-kupu dilakukan dengan metode kombinasi yaitu gabungan dari metode kualitatif dan metode kuantitatif. Teknik analisis data yang digunakan yaitu indeks keanekaragaman jenis, indeks kemerataan jenis, dan indeks kekayaan jenis. Hasil penelitian menunjukkan bahwa jenis vegetasi yang ditemukan di sungai Oba diantaranya Pinang hutan, Sukun hutan (Artocarpus elasticus). Aren (Arenga pinata), Siri hutan (Piper aduncun), Kayu soro (Ficus exasperate), paku raja (Polystichum setiferm), Kananga (Cananga odorata), Gosale (Syzygium malaccense), Jabon (Antochepalus cadamba), Sengon (Albizia chinensis), Benuang laki (Duabanga moluccana), Binuang bini (Octomeles sumatrana), Pisang hutan (Musa acuminate), Matoa (Pometia pinnata), Beringin (Ficus benjamina), Rambutan (Nephelium lappaceum), Awar-awar (Ficus septica), Gofasa (Vitex cofassus), Kayu bugis (Koordersiodendron pinnatum), dan Pala (Myristica fragrans). Suhu udara berkisar antara 26-30 ºC dan kelembapan udara berkisar antara 75-80%. Nilai indeks keanekaragaman kupu-kupu di Sungai Oba yakni 2,53 sehingga tergolong sedang.
Social Skills Development for Students with Autism Spectrum Disorders in Inclusive Classrooms by Social Stories Approach
Authors: Wongkittirungrueang Sawitree , Sanrattana Unchalee , Warnset Somporn ,
Published: June 2024

The research aimed to employ the social story approach to enhance the social skills of students with autism spectrum disorders (ASDs), particularly those in the high-functioning category, within a full inclusion setting. Despite their enrollment in an inclusive classroom, these students encountered challenges related to social skills, including the recognition of emotional expressions, interactions with peers, appropriate behavior in societal settings, collaboration with others, and adherence to social norms. The research utilized the Individual Implementation Plan (IIP) for social stories and a social skill's observation form tailored for students with ASDs. This study employed a single-subject across behaviors, or an A1-B-A2 design, dividing the intervention into three stages: the initial baseline (A1), the subsequent treatment phase (B), and the final withdrawal or post-trial period (A2). The findings indicated that following the implementation of social stories, students with ASDs in inclusive classrooms exhibited significant improvements in recognizing others' emotions and expressions, engaging in social interactions, demonstrating appropriate behavior in various societal contexts, collaborating effectively with peers, and adhering to social rules. Notably, these advancements were most pronounced during the withdrawal or post-trial period (A2).
Coleções didáticas para o ensino de botânica no Instituto Federal de Sergipe - campus Aracaju
Authors: Brandão Érika Cristina Teixeira dos Anjos , Dantas Camilla Silen de Almeida , Oliveira Marla Ibrahim Uehbe de , Silva Irane Gonçalves da
Published: June 2024

Recursos didáticos são ferramentas auxiliares na prática docente, estimulando a aprendizagem e promovendo a superação de fenômenos como a impercepção botânica. O objetivo deste trabalho foi relatar a implementação de quatro tipos de coleções em um campus do IFS: herbário, jardim didático, lâminas de histologia vegetal e modelos didáticos. Para isso, foram realizadas pesquisas em literatura especializada; seleção de plantas para o jardim didático e para as lâminas de histologia; e seleção de estruturas vegetais para os modelos didáticos. Para a confecção do herbário, foram realizadas coletas de plantas e montagem de exsicatas em parceria com outra instituição federal. O herbário possui 56 exsicatas; o jardim didático, 26 espécimes da flora regional; a coleção de lâminas conta com 50 peças; e a coleção de modelos, seis estruturas vegetais. Espera-se que a utilização destas coleções contribua para uma aprendizagem condizente com a formação de cidadãos conscientes do seu papel junto à natureza.
Palinotaxonomia de espécies de Dipteryx Schreb. (Fabaceae – Papilionoideae) ocorrentes no Brasil
Authors: Araújo Natalia Moura de , Abreu Vanessa Holanda Righetti de
Published: June 2024

O gênero Dipteryx Schreb. (Papilionoideae) conhecido principalmente como cumaru, compreende espécies de árvores economicamente importantes que têm chamado a atenção de vários países. O objetivo deste estudo foi avaliar a morfologia polínica das espécies do gênero Dipteryx ocorrentes no Brasil visando corroborar com a classificação e circunscrição das espécies do gênero. O material polínico das espécies foi retirado de exsicatas depositadas no herbário do Instituto Nacional de Pesquisas da Amazônia (INPA). Os grãos de pólen foram tratados por meio de acetólise, mensurados, descritos quanto ao tamanho, âmbito, forma e ornamentação e posteriormente, fotomicrografados. Constatou-se que os grãos de pólen são mônades, tamanho variando de médios à grandes, isopolares, com forma suboblata a prolata-esferoidal, âmbito subtriangular a triangular, 3-colporados, exina microrreticulada a reticulada. As espécies de Dipteryx apresentaram grãos de pólen com pequenas variações morfológicas, especialmente quanto à ornamentação da exina. Assim, a morfologia polínica do gênero, poderá subsidiar futuros trabalhos nas diversas áreas da Palinologia.
Introducing new dimensions in pediatric oncologic surgery
Authors:   , Fitski Matthijs
Published: June 2024

In the Netherlands, approximately 30 children per year are diagnosed with kidney cancer. For most patients, the entire kidney along with the tumor is surgically removed (Total Nephrectomy, TN). For the other patients, the tumor is removed from the kidney (Nephron-Sparing Surgery, NSS). NSS is performed on children with bilateral tumors or children with specific overgrowth syndromes. NSS is only performed under very strict conditions in children who have a unilateral tumor and no overgrowth syndromes. This is because NSS is very challenging, and the tumor is not completely removed in 13.3% to 36.4% of the surgeries. Despite this, NSS is expected to have benefits compared to TN. For instance, the likelihood of chronic kidney disease later in life could be reduced.

In this dissertation, we conducted research to make NSS more feasible for this specific patient group. First, we examined which patients in this group could be eligible for NSS surgically. There are oncological guidelines for this decision, but no surgical guidelines exist yet. Therefore, we conducted an international Delphi consensus study among experts in the field of NSS. The other part of our study focused on technical improvements in NSS. For this, we developed and implemented 3D technology in our surgical care. Using 3D models, a surgeon can prepare for an operation and gain a better understanding of the anatomical relationships of a patient's tumor. Additionally, we worked on surgical navigation using a holographic 3D model. The 3D model is projected holographically into a patient. This should make it easier to locate the tumor and improve the surgeon's depth perception.

Following these two research directions, I looked at the impact of 3D models on surgical outcomes for patients who underwent NSS at the Princess Máxima Center. We observed a trend in decreased unexpected incomplete tumor removal in surgeries where the surgeon used a 3D model during preparation.

In summary, we have taken steps to improve surgical decision-making and techniques for children with kidney tumors where nephron-sparing surgery could be possible.
DIGITAL TECHNOLOGIES: CULTURAL APPROACH
Authors: POPKO LYUDMYLA , KIZIM SVITLANA , MIRONOVA TATIANA , BROVCHAK LIUDMYLA , ZHADEYKO OLEKSIY
Published: June 2024

The article aims to analyze conceptual approaches to the interdisciplinary direction of digital culture, its main models, and practices. Digital technologies transformed all spheres of society: culture, economy, political sphere, science, and education and gave rise to the phenomenon of digital culture. Various conceptual models and practices characterize its manifestation in each of these areas. The article presents the definition of digital culture as an interdisciplinary area of research, considers the specifics of its main sublevels, and explores the trends in its development as a practical implementation of its conceptual models in social ideology, university education, socio-humanitarian discourse, and art. The penetration of information technologies into the activities of cultural institutions is universal. Therefore, the article presents a new experience in implementing human activity, which became possible due to the introduction of digital and information and communication technologies. Particular attention is drawn to the need to use a holistic and systematic approach to digital communication to update the cultural experience, upbringing, and education of a trained viewer and listener.
OPERATIONAL MANAGEMENT OF SERVICE FIELD ENTERPRISES UNDER THE CONDITIONS OF DIGITALIZATION IN THE SYSTEM OF SUSTAINABLE DEVELOPMENT
Authors: KRASNOMOVETS VIKTORIIA , MAZNIEV IEVGEN , SARANA OLEKSANDR , SHULHA OLHA , KHOMIUK NATALIIA , ARTEMCHUK LIUDMYLA
Published: June 2024

The article explores the unique impact of digitalization on the operational management of service enterprises, underscoring the importance of strategies and tools for optimizing business processes. These tools empower enterprises to effectively use digital technologies, increasing productivity while reducing negative environmental impact. The study also highlights the crucial role of innovative solutions in ensuring sustainable development and how digital technologies can enhance service quality, meeting consumers’ evolving needs. The article concludes by emphasizing the necessity of involving the audience in implementing digital innovations into the management system of service enterprises, aligning with the contemporary demands of efficient and sustainable development.
RANCANG BANGUN TRAINER PENGEREMAN SEPEDA MOTOR TIPE DISK BRAKE DENGAN MENGGUNAKAN SINGLE PISTON CALIPER
Authors: Erdin Dwi Setiawan  , Mohammad Munib Rosadi  , Retno Eka Pramitasari
Published: June 2024

Rem merupakan sebuah komponen keselamatan pada seorang pengendara, dengan adanya komponen tersebut mampu mencegah terjadinya hal yang tidak diinginkan seperti kecelakaan. Secara umum rem berkembang pada kendaraan roda dua yaitu rem cakram (disk brake) dan rem tromol (drum brake). Tujuan dari penelitian ini adalah untuk mendeskripsikan desain trainer pengereman dan menganalisa performa pada trainer pengereman. Penelitian dari rancang bangun trainer pengereman sepeda motor tipe disk brake dengan menggunakan single piston caliper menggunakan metode R&D (research and development. Pada proses perancangan diawali dengan pembuatan desain alat meliputi, desain rangka, pulley, v-belt, pillow block dan batang as. Kemudian adanya proses perakitan dan pengujian alat mahasiswa mampu mengetahui sistem kerja pada pengereman tipe disk brake.
PENGARUH KUAT ARUS PENGELASAN SMAW DAN VARIASI SUDUT PENGELASAN ELEKTRODA TERHADAP KEKUATAN TARIK HASIL PENGELASAN PADA BESI HOLLOW HITAM
Authors: Mochamad Latief Rizaldi  , Fajar Satriya Hadi  , Dian Anisa Rokhmah Wati  , Ali Hasbi Ramadani
Published: June 2024

Penelitian ini dilatar belakangi oleh Peneliti yang tertarik untuk menguji pada material besi hollow dengan melakukan perbandingan terhadap pengaruh arus pengelasan dan sudut pengelasan menggunakan uji tarik. Penelitian ini melakukan beberapa variasi arus dan variasi sudut pengelasan elektroda. Untuk variasi arus yaitu menggunakan arus 80 Ampere, 100 Ampere dan sudut elektroda menggunakan 70o ,80o ,90o serta menggunakan bahan besi hollow jenis hitam. Dibalik tujuan ini berfungsi apakah ada pengaruh dari pengelasan sambungan besi hollow terhadap perbedaan arus dan sudut elektroda pada proses pengelasan dalam uji tarik. Hasil penelitian menunjukan. Hasil dari penelitian menunjukan bahwa ada pengaruh dari hasil penelitian ini terbukti dengan kuat arus 80 Ampere dengan sudut elektroda 70o mendapatkan nilai 323.9 Mpa, dibandingkan menggunakan kuat arus 100 Ampere sudut 80o dengan hasil 306.3 Mpa.
The relationship between neighborhood economic deprivation and asthma-associated emergency department visits in Maryland
Authors: Akinyemi Oluwasegun , Weldeslase Terhas , Odusanya Eunice , Fasokun Mojisola , Agboola Bukola , Andine Tsion , Ayeni Esther , Michael Miriam , Hughes Kakra
Published: June 2024

Background

Asthma represents a substantial public health challenge in the United States, affecting over 25 million adults. This study investigates the impact of neighborhood economic deprivation on asthma-associated Emergency Department (ED) visits in Maryland, using the Distressed Communities Index (DCI) for analysis.

Methods

A retrospective analysis of Maryland's Emergency Department Databases from January 2018 to December 2020 was conducted, focusing on asthma-associated ED visits.

Results

The study involved 185,317 ED visits, majority of which were females (56.3%) and non-Hispanic whites (65.2%). A significant association was found between increased neighborhood socioeconomic deprivation and asthma-related ED visits. The poorest neighborhoods showed the highest rates of such visits. Compared to prosperous areas, neighborhoods classified from Comfortable to Distressed had progressively higher odds for asthma-related ED visits (Comfortable: OR = 1.14, Distressed OR = 1.65). Other significant asthma predictors included obesity, female gender, tobacco smoking, and older age.

Conclusion

There is a substantive association between higher asthma-related ED visits and high neighborhood economic deprivation, underscoring the impact of socioeconomic factors on health outcomes.

Public health implications

Addressing healthcare disparities and improving access to care in economically distressed neighborhoods is crucial. Targeted interventions, such as community health clinics and asthma education programs, can help mitigate the impact of neighborhood disadvantage.
On-farm study: cytokine profiles and vaginal microbiome of Bos indicus cattle before artificial insemination
Authors: Smith Molly S. , Soffa Dallas R. , McAnally Brooke E. , Hickman-Brown Kyle J. , Stockland Erin L. , Poole Rebecca K.
Published: June 2024

Prior studies in beef cattle have shown shifts in the reproductive microbiome prior to artificial insemination (AI), yet few have characterized both the reproductive microbiome and immune responses prior to AI, particularly in purebred Bos indicus. Therefore, the aim of this study was to characterize the circulating cytokines and the vaginal microbiome of Bos indicus females prior to fixed-time artificial insemination (FTAI). Bos indicus females (n = 79) on four separate ranch operations within a 20-mile radius in East Texas were subjected to the 7-day CO-Synch + controlled intervaginal drug-releasing (CIDR) protocol beginning on day (d)-9 with FTAI on d0. Blood samples were collected on d-9 (CIDR IN), d-2 (CIDR OUT), and d0 (AI DAY) for cytokine concentration analyses using the RayBiotech Quantibody Bovine Cytokine Array Q1 kit per the manufacturer’s instructions. Sterile vaginal swabs were inserted past the vulva, rotated, and stored at -80°C for microbiome analysis. Bacterial community analyses targeted the V4 hypervariable region of the 16S rRNA gene. Pregnancy status was determined by transrectal ultrasonography approximately 60 days after FTAI for resulting open females (n = 45) and pregnant females (n = 34). Regardless of pregnancy status, the vaginal relative abundance of Firmicutes differed between CIDR IN, CIDR OUT, and AI DAY (63.74% vs. 28.31% vs. 60.86% ± 3.66%, respectively; P < 0.01). Genera with phylum Firmicutes including Ruminococcus, Clostridium, Blautia, Butyrvibrio, and Mogibacterium followed a similar trend (P < 0.05). Butyrvibrio tended to have greater relative abundance in the vaginal samples of Cows than Heifers (4.17% ± 0.75% vs. 3.26% ± 0.77%; P = 0.07). Concentrations of the interferon (IFN)γ (2005.98 ± 471.94 pg/mL vs. 1185.40 ± 482.65 pg/mL; P < 0.01), interleukin (IL)1F5 (153.89 ± 141.07 pg/mL vs. 627.30 ± 149.28 pg/mL; P < 0.01), and interferon gamma-induced protein (IP)10 (9363.26 ± 2929.83 pg/mL vs. 5905.53 ± 2983.60 pg/mL; P = 0.05) were greater in Cows than Heifers. There was a parity-by-status interaction for IP10, with Open Cows having the greatest concentration compared all other groups (P < 0.05). These results indicate differences in circulating cytokines and shifts in the vaginal microbiome for in Bos indicus cattle prior to FTAI.
Comparative FEM study on intervertebral disc modeling: Holzapfel-Gasser-Ogden vs. structural rebars
Authors: Gruber Gabriel , Nicolini Luis Fernando , Ribeiro Marx , Lerchl Tanja , Wilke Hans-Joachim , Jaramillo Héctor Enrique , Senner Veit , Kirschke Jan S. , Nispel Kati
Published: June 2024

Introduction: Numerical modeling of the intervertebral disc (IVD) is challenging due to its complex and heterogeneous structure, requiring careful selection of constitutive models and material properties. A critical aspect of such modeling is the representation of annulus fibers, which significantly impact IVD biomechanics. This study presents a comparative analysis of different methods for fiber reinforcement in the annulus fibrosus of a finite element (FE) model of the human IVD.

Methods: We utilized a reconstructed L4-L5 IVD geometry to compare three fiber modeling approaches: the anisotropic Holzapfel-Gasser-Ogden (HGO) model (HGO fiber model) and two sets of structural rebar elements with linear-elastic (linear rebar model) and hyperelastic (nonlinear rebar model) material definitions, respectively. Prior to calibration, we conducted a sensitivity analysis to identify the most important model parameters to be calibrated and improve the efficiency of the calibration. Calibration was performed using a genetic algorithm and in vitro range of motion (RoM) data from a published study with eight specimens tested under four loading scenarios. For validation, intradiscal pressure (IDP) measurements from the same study were used, along with additional RoM data from a separate publication involving five specimens subjected to four different loading conditions.

Results: The sensitivity analysis revealed that most parameters, except for the Poisson ratio of the annulus fibers and C01 from the nucleus, significantly affected the RoM and IDP outcomes. Upon calibration, the HGO fiber model demonstrated the highest accuracy (R2 = 0.95), followed by the linear (R2 = 0.89) and nonlinear rebar models (R2 = 0.87). During the validation phase, the HGO fiber model maintained its high accuracy (RoM R2 = 0.85; IDP R2 = 0.87), while the linear and nonlinear rebar models had lower validation scores (RoM R2 = 0.71 and 0.69; IDP R2 = 0.86 and 0.8, respectively).

Discussion: The results of the study demonstrate a successful calibration process that established good agreement with experimental data. Based on our findings, the HGO fiber model appears to be a more suitable option for accurate IVD FE modeling considering its higher fidelity in simulation results and computational efficiency.
Redefining vascular repair: revealing cellular responses on PEUU—gelatin electrospun vascular grafts for endothelialization and immune responses on in vitro models
Authors: Rodríguez-Soto María A. , Riveros-Cortés Alejandra , Orjuela-Garzón Ian C. , Fernández-Calderón Inés María , Rodríguez Cristian F. , Vargas Natalia Suárez , Ostos Carlos , Camargo Carolina Muñoz , Cruz Juan C. , Kim Seungil , D’Amore Antonio , Wagner William R. , Briceño Juan C.
Published: June 2024

Tissue-engineered vascular grafts (TEVGs) poised for regenerative applications are central to effective vascular repair, with their efficacy being significantly influenced by scaffold architecture and the strategic distribution of bioactive molecules either embedded within the scaffold or elicited from responsive tissues. Despite substantial advancements over recent decades, a thorough understanding of the critical cellular dynamics for clinical success remains to be fully elucidated. Graft failure, often ascribed to thrombogenesis, intimal hyperplasia, or calcification, is predominantly linked to improperly modulated inflammatory reactions. The orchestrated behavior of repopulating cells is crucial for both initial endothelialization and the subsequent differentiation of vascular wall stem cells into functional phenotypes. This necessitates the TEVG to provide an optimal milieu wherein immune cells can promote early angiogenesis and cell recruitment, all while averting persistent inflammation. In this study, we present an innovative TEVG designed to enhance cellular responses by integrating a physicochemical gradient through a multilayered structure utilizing synthetic (poly (ester urethane urea), PEUU) and natural polymers (Gelatin B), thereby modulating inflammatory reactions. The luminal surface is functionalized with a four-arm polyethylene glycol (P4A) to mitigate thrombogenesis, while the incorporation of adhesive peptides (RGD/SV) fosters the adhesion and maturation of functional endothelial cells. The resultant multilayered TEVG, with a diameter of 3.0 cm and a length of 11 cm, exhibits differential porosity along its layers and mechanical properties commensurate with those of native porcine carotid arteries. Analyses indicate high biocompatibility and low thrombogenicity while enabling luminal endothelialization and functional phenotypic behavior, thus limiting inflammation in in-vitro models. The vascular wall demonstrated low immunogenicity with an initial acute inflammatory phase, transitioning towards a pro-regenerative M2 macrophage-predominant phase. These findings underscore the potential of the designed TEVG in inducing favorable immunomodulatory and pro-regenerative environments, thus holding promise for future clinical applications in vascular tissue engineering.
Differential modulation of cell morphology, migration, and Neuropilin-1 expression in cancer and non-cancer cell lines by substrate stiffness
Authors: Vela-Alcántara Ana Monserrat , Santiago-García Juan , Barragán-Palacios Madeleine , León-Chacón Aylin , Domínguez-Pantoja Marilú , Barceinas-Dávila Irene , Juárez-Aguilar Enrique , Tamariz Elisa
Published: June 2024

Physical changes in the tumor microenvironment, such as increased stiffness, regulate cancer hallmarks and play an essential role in gene expression, cell morphology, migration, and malignancy. However, the response of cancer cells to stiffness is not homogeneous and varies depending on the cell type and its mechanosensitivity. In this study, we investigated the differential responses of cervical (HeLa) and prostate (PC-3) cancer cell lines, as well as non-tumoral cell lines (HEK293 and HPrEC), to stiffness using polyacrylamide hydrogels mimicking normal and tumoral tissues. We analyzed cell morphology, migration, and the expression of neuropilin 1 (NRP1), a receptor involved in angiogenesis, cell migration, and extracellular matrix remodeling, known to be associated with cancer progression and poor prognosis. Our findings reveal that NRP1 expression increases on substrates mimicking the high stiffness characteristic of tumoral tissue in the non-tumoral cell lines HPrEC and HEK293. Conversely, in tumoral PC-3 cells, stiffness resembling normal prostate tissue induces an earlier and more sustained expression of NRP1. Furthermore, we observed that stiffness influences cell spreading, pseudopodia formation, and the mode of cell protrusion during migration. Soft substrates predominantly trigger bleb cell protrusion, while pseudopodia protrusions increase on substrates mimicking normal and tumor-like stiffnesses in HPrEC cells compared to PC-3 cells. Stiffer substrates also enhance the percentage of migratory cells, as well as their velocity and total displacement, in both non-tumoral and tumoral prostate cells. However, they only improve the persistence of migration in tumoral PC-3 cells. Moreover, we found that NRP1 co-localizes with actin, and its suppression impairs tumoral PC-3 spreading while decreasing pseudopodia protrusion mode. Our results suggest that the modulation of NRP1 expression by the stiffness can be a feedback loop to promote malignancy in non-tumoral and cancer cells, contingent upon the mechanosensitivity of the cells.
Computational modelling of micropolar blood-based magnetised hybrid nanofluid flow over a porous curved surface in the presence of artificial bacteria
Authors: Deebani Wejdan , Shah Zahir , Rooman Muhammad , Khan Naeem Ullah , Vrinceanu Narcisa , Shutaywi Meshal
Published: June 2024

This work provides a brief comparative analysis of the influence of heat creation on micropolar blood-based unsteady magnetised hybrid nanofluid flow over a curved surface. The Powell–Eyring fluid model was applied for modelling purposes, and this work accounted for the impacts of both viscous dissipation and Joule heating. By investigating the behaviours of Ag and TiO2 nanoparticles dispersed in blood, we aimed to understand the intricate phenomenon of hybridisation. A mathematical framework was created in accordance with the fundamental flow assumptions to build the model. Then, the model was made dimensionless using similarity transformations. The problem of a dimensionless system was then effectively addressed using the homotopy analysis technique. A cylindrical surface was used to calculate the flow quantities, and the outcomes were visualised using graphs and tables. Additionally, a study was conducted to evaluate skin friction and heat transfer in relation to blood flow dynamics; heat transmission was enhanced to raise the Biot number values. According to the findings of this study, increasing the values of the unstable parameters results in increase of the blood velocity profile.
A novel pathogenic species of genus Stenotrophomonas: Stenotrophomonas pigmentata sp. nov
Authors: Li Yue , Yu Zelin , Fan Xueting , Xu Da , Liu Haican , Zhao Xiuqin , Wang Ruibai
Published: June 2024

Introduction

Stenotrophomonas is a prominent genus owing to its dual nature. Species of this genus have many applications in industry and agriculture as plant growth-promoting rhizobacteria and microbial biological control agents, whereas species such as Stenotrophomonas maltophilia are considered one of the leading gram-negative multi-drug-resistant bacterial pathogens because of their high contribution to the increase in crude mortality and significant clinical challenge. Pathogenic Stenotrophomonas species and most clinical isolates belong to the Stenotrophomonas maltophilia complex (SMc). However, a strain highly homologous to S. terrae was isolated from a patient with pulmonary tuberculosis (TB), which aroused our interest, as S. terrae belongs to a relatively distant clade from SMc and there have been no human association reports.

Methods

The pathogenicity, immunological and biochemical characteristics of 610A2T were systematically evaluated.

Results

610A2T is a new species of genus Stenotrophomonas, which is named as Stenotrophomonas pigmentata sp. nov. for its obvious brown water-soluble pigment. 610A2T is pathogenic and caused significant weight loss, pulmonary congestion, and blood transmission in mice because it has multiple virulence factors, haemolysis, and strong biofilm formation abilities. In addition, the cytokine response induced by this strain was similar to that observed in patients with TB, and the strain was resistant to half of the anti-TB drugs.

Conclusions

The pathogenicity of 610A2T may not be weaker than that of S. maltophilia. Its isolation extended the opportunistic pathogenic species to all 3 major clades of the genus Stenotrophomonas, indicating that the clinical importance of species of Stenotrophomonas other than S. maltophilia and potential risks to biological safety associated with the use of Stenotrophomonas require more attention.
Design and simulation of a new QCA-based low-power universal gate
Authors: Sadrarhami Hamidreza , Zanjani S. Mohammadali , Dolatshahi Mehdi , Barekatain Behrang
Published: June 2024

Quantum-dot Cellular Automata (QCA) is recognized in electronics for its low power consumption and high-density capabilities, emerging as a potential substitute for CMOS technology. GDI (Gate Diffusion Input) technology is featured as an innovative approach for enhancing power efficiency and spatial optimization in digital circuits. This study introduces an advanced four-input Improved Gate Diffusion Input (IGDI) design specifically for QCA technology as a universal gate. A key feature of the proposed 10-cell block is the absence of cross-wiring, which significantly enhances the circuit’s operational efficiency. Its universal cell nature allows for the carrying out of various logical gates by merely altering input values, without necessitating any structural redesign. The proposed design showcases notable advancements over prior models, including a reduced cell count by 17%, a 29% decrease in total energy usage, and a 44% reduction in average energy loss. This innovative IGDI design efficiently executes 21 combinational and various sequential functions. Simulations in 18 nm technology, accompanied by energy consumption analyses, demonstrate this design’s superior performance compared to existing models in key areas such as multiplexers, comparators, and memory circuits, alongside a significant reduction in cell count.
Case Report: Successful retrieval of a migrated left atrial appendage occluder through the synergistic use of a guidewire and a gooseneck snare
Authors: Bao Yue , Yi Hongwei , Ma Jun , Han Hongwei
Published: June 2024

Left atrial appendage occluder (LAAO) dislodgement with embolization is a rare occurrence. If the LAAO migrates into the left atrium or ventricle, it can lead to acute heart failure or even death in a person, necessitating urgent surgical intervention. Currently, most cases of LAAO dislodgement are managed through open-heart surgery, while percutaneous retrieval of the LAAO has been reported only in a few cases with limited associated experience. This article reports a case of a patient in whom a migrated LACbes device was successfully retrieved using a catheter-based approach, demonstrating an innovative and minimally invasive treatment strategy.
Unilateral boundary time series forecasting
Authors: Chang Chao-Min , Li Cheng-Te , Lin Shou-De
Published: June 2024

Time series forecasting is an essential tool across numerous domains, yet traditional models often falter when faced with unilateral boundary conditions, where data is systematically overestimated or underestimated. This paper introduces a novel approach to the task of unilateral boundary time series forecasting. Our research bridges the gap in existing methods by proposing a specialized framework to accurately forecast within these skewed datasets. The cornerstone of our approach is the unilateral mean square error (UMSE), an asymmetric loss function that strategically addresses underestimation biases in training data, improving the precision of forecasts. We further enhance model performance through the implementation of a dual model structure that processes underestimated and accurately estimated data points separately, allowing for a nuanced analysis of the data trends. Additionally, feature reconstruction is employed to recapture obscured dynamics, ensuring a comprehensive understanding of the data. We demonstrate the effectiveness of our methods through extensive experimentation with LightGBM and GRU models across diverse datasets, showcasing superior accuracy and robustness in comparison to traditional models and existing methods. Our findings not only validate the efficacy of our approach but also reveal its model-independence and broad applicability. This work lays the groundwork for future research in this domain, opening new avenues for sophisticated analytical models in various industries where precise time series forecasting is crucial.
Age and orbital forcing in the upper Silurian Cellon section (Carnic Alps, Austria) uncovered using the WaverideR R package
Authors: Arts Michiel , Corradini Carlo , Pondrelli Monica , Pas Damien , Da Silva Anne-Christine
Published: June 2024

The type-Silurian Cellon section in the Carnic Alps in Austria underpins much of the current Silurian conodont zonations, forming the basis for the Silurian timescale. However, the Silurian record of the Cellon section lacks radiometric and astrochronological age constraints, making it difficult to gain insights into the processes pacing Silurian (anoxic) events. To attain age constraints and investigate the pacing Silurian (anoxic) events by astronomical cycles, a cyclostratigraphic study was conducted on high-resolution pXRF (CaO, Al2O3, and Fe2O3) and induration records spanning the Ludlow and Pridoli parts of the Cellon section. Astronomical cycles ranging from precession to the 405-kyr eccentricity cycle were first recognised visually in the field and in proxy records. The visual detection of astronomical cycles served as an input for the WaverideR R package, enabling the tracking of the 405-kyr eccentricity period in each proxy’s continous wavelet transform scalograms. These tracked period curves were combined with external age controls through multiple Monte Carlo simulations, generating an (absolute) age model. This age model is used to assign ages and durations and their respective uncertainties to a hiatus in the Ludfordian, conodont zones, lithological units, geochronological units and events, yielding new ages for Silurian stage boundaries (e.g., Gorstian-Ludfordian boundary at 425.92 ± 0.65 Ma, the Ludfordian-Pridoli boundary at 423.03 ± 0.53 Ma, the Silurian-Devonian boundary at 418.86 ± 1.02 Ma), and new durations for the Ludfordian at 2.89 ± 0.35 Myr and Pridoli at 4.24 ± 0.46 Myr. Furthermore, the imprint of astronomical cycles in the Cellon section itself indicates that the Linde, Klev and Silurian-Devonian boundary events all occur after a 2.4-Myr eccentricity node, indicating pacing by astronomical forcing, similar to other Devonian and Cretaceous anoxic events. The Lau event, however, does not appear to coincide with a 2.4-Myr eccentricity node.
The application of the K-class system in mine seismicity
Authors: Wang Chao , Zhan Kai , Zhao Xipo , Xu Rui , Kong Chao
Published: June 2024

As seismology has evolved, scientists have developed various earthquake magnitude scales tailored for specific scenarios, including Richter magnitude (ML) for local, moderate quakes, moment magnitude (Mw) for large-scale seismic events, energy magnitude (Me), which correlates directly with the total energy released, and duration magnitude (MD) for quickly assessing shallow or nearby seismic activities. Despite these advances, these scales were not specifically designed to address the unique challenges posed by mine earthquakes, particularly in coal mine environments where seismic events are typically of lower energy and lower perceptibility but have potentially significant impacts. This study applies the K-class magnitude system for the first time to address significant discrepancies in magnitude values for the same seismic events in mine earthquakes, particularly in coal mines when different formulas are used. However, because current seismic monitoring systems in domestic coal mines continue to employ a variety of magnitude scales, we have developed a corrected Richter scale (MLc) specifically tailored to the geological conditions of coal mines. This adaptation facilitates the conversion to and from the K class, enhancing the accuracy and relevance of seismic assessments in these environments. Therefore, we conducted continuous blasting experiments at the Dongtan Coal Mine and derived the corrected Richter magnitude MLc. Based on data analysis, we fitted the energy attenuation coefficient and compared the K values with four types of traditional magnitude results. Finally, we derived the conversion relationship between the K class and the corrected Richter magnitude MLc, establishing a mine earthquake measurement system. The successful application of the K class in coal mines suggests its potential to be extended to the entire field of induced microseismicity, including mining-induced microseismicity, shale gas extraction-induced microseismicity, and oil extraction-induced microseismicity, providing valuable insights and tools for a broader range of geophysical research and industry practices.
Intermediate complexity atmospheric modeling in complex terrain: is it right?
Authors: Reynolds Dylan , Haugeneder Michael , Lehning Michael , Mott Rebecca
Published: June 2024

Dynamic downscaling of atmospheric forcing data to the hectometer resolution has shown increases in accuracy for landsurface models, but at great computational cost. Here we present a validation of a novel intermediate complexity atmospheric model, HICAR, developed for hectometer scale applications. HICAR can run more than 500x faster than conventional atmospheric models, while containing many of the same physics parameterizations. Station measurements of air temperature, wind speed, and radiation, in combination with data from a scanning Doppler wind LiDAR, are compared to 50 m resolution HICAR output during late spring. We examine the model’s performance over bare ground and melting snow. The model shows a smaller root mean squared error in 2 m air temperature than the driving model, and approximates the 3D flow features present around ridges and along slopes. Timing and magnitude of changes in shortwave and longwave radiation also show agreement with measurements. Nocturnal cooling during clear nights is overestimated at the snow covered site. Additionally, the thermal wind parameterization employed by the model typically produces excessively strong surface winds, driven in part by this excessive nocturnal cooling over snow. These findings highlight the utility of HICAR as a tool for dynamically downscaling forcing datasets, and expose the need for improvements to the snow model used in HICAR.
Acoustic emission characteristics and fracture mechanism of sandstone in open-pit mines under different types of cyclic loads
Authors: Zhang Qing , Zhang Ling , Jiang Xutong
Published: June 2024

Rock mass is one of the most important load-bearing media in geotechnical engineering. It has been continually vulnerable to geological tectonic movements, natural calamities, and human excavation activities. Its inherent weak surfaces such as primary pores, joints, and fissures have resulted in varying damage degrees. In mining operations, the damaged rock mass has a variety of negative impacts on the stability of its overlying structures and is frequently disturbed by the load. To study the damage law of rock mass under cyclic loading, in this paper, an acoustic emission (AE) device was employed to monitor the rock under the action of two types of cyclic loads: the variable upper and lower pre-loads, and the fixed upper and lower pre-loads. The damage of the loaded rock was split into three stages in this research, based on the features of the AE signals of the rock under uniaxial load, and the damage evolution of the loaded rock was analyzed in distinct stages. The AE signals of the rock under cyclic loading were mainly emitted in the first loading stage. When the stress did not exceed the maximum stress value in the stress history of the loaded rock, few new AE event was generated in the loaded rock. After the low-frequency cyclic static load, the AE signals varied with the load-bearing stress of the rock during the whole process from initial loading to failure, which was consistent with the characteristics of the AE signals of the loaded rock. The research results can be adapted to rock mass in open-pit mines stability analysis and risk prediction while providing some references for the early warning and danger relief of rock masses in engineering.
No child left behind, literacy challenges ahead: a focus on the Philippines
Authors: Gatcho Al Ryanne Gabonada , Manuel Jeremiah Paul Giron , Hajan Bonjovi Hassan
Published: June 2024

The Sustainable Development Goal 4 has commenced a global mandate to provide equitable access to quality education for everyone. In the Philippines, SDG 4 inaugurates the No Child Left Behind (NCLB) Policy. This brief argues that while the NCLB has ensured equal access to quality literacy education, it poses socioeconomic-based challenges, declining rate of parental involvement in their children’s schooling, overemphasis on standardized tests, and the lack of community involvement towards literacy programs. The Holistic Literacy Enhancement Program (HLEP) is proposed in this paper to help address these challenges to NCLB. HLEP presents policy implications that could assist the NCLB in more efficient and effective implementation: equitable resource allocation, parental and community engagement, and culturally and linguistically relevant assessment tools.
Understanding self-regulation strategies in problem-based learning through dispositional learning analytics
Authors: Tempelaar Dirk , Bátori Anikó , Giesbers Bas
Published: June 2024

In the ongoing discussion about how learning analytics can effectively support self-regulated student learning and which types of data are most suitable for this purpose, this empirical study aligns with the framework who advocated the inclusion of both behavioral trace data and survey data in learning analytics studies. By incorporating learning dispositions in our learning analytics modeling, this research aims to investigate and understand how students engage with learning tasks, tools, and materials in their academic endeavors. This is achieved by analyzing trace data, which captures digital footprints of students’ interactions with digital tools, along with survey responses from the Study of Learning Questionnaire (SLQ), to comprehensively examine their preferred learning strategies. Additionally, the study explores the relationship between these strategies and students’ learning dispositions measured at the start of the course. An innovative aspect of this investigation lies in its emphasis on understanding how learning dispositions act as antecedents and potentially predict the utilization of specific learning strategies. The data is scrutinized to identify patterns and clusters of such patterns between students’ learning disposition and their preferred strategies. Data is gathered from two cohorts of students, comprising 2,400 first year students. This analytical approach aims to uncover predictive insights, offering potential indicators to predict and understand students’ learning strategy preferences, which holds value for teachers, educational scientists, and educational designers. Understanding students’ regulation of their own learning process holds promise to recognize students with less beneficial learning strategies and target interventions aimed to improve these. A crucial takeaway from our research underscores the significance of flexibility, which entails the ability to adjust preferred learning strategies according to the learning environment. While it is imperative to instruct our students in deep learning strategies and encourage autonomous regulation of learning, this should not come at the expense of acknowledging situations where surface strategies and controlled regulation may prove to be more effective.