bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrated Enhanced Cognitive Behavioural (I-CBTE) therapy significantly improves effectiveness of inpatient treatment of anorexia nervosa in real life settings
Authors: Ibrahim Ali , Ryan Sharon , Viljoen David , Tutisani Ellen , Gardner Lucy , Collins Lorna , Ayton Agnes
Published: January 2022

Background Inpatient treatment of anorexia nervosa (AN) often results in poor outcomes. To address this, the Oxford service has adapted the multistep enhanced cognitive behavioural (CBTE) treatment model, first developed in Italy, to an NHS setting.Aim: to evaluate short- and long-term outcomes of Integrated CBTE (I-CBTE) with alternative treatment models in routine clinical practice. Methods This is a longitudinal cohort study, involving all adults with AN admitted from a geographical area in England covering a total population of 3.5 million between 2017-2020 using routinely collected data. We compared treatment as usual (TAU) with the Oxford model, which included: 1. short term planned admission with partial weight restoration; 2. I-CBTE (weight restoration defined as BMI 20, combined with 7 weeks day treatment followed by outpatient CBTE); 3. standalone inpatient CBTE. Primary outcome measures (min. 1 year after discharge from hospital) were defined as: 1. good outcome: BMI>19.5 and no binge purging behaviour; 2. poor outcome: BMI<19.5 and/or binge purging; 3. readmission; or 4. deceased.Secondary outcomes were BMI on admission and discharge, and length of stay. Results 212 patients were admitted to 15 specialist units in the UK depending on availability: 120 to Oxford and 92 elsewhere. The mean age: 28.9 (18-60) years, mean BMI: 14.4. There were no significant differences in baseline parameters between patients admitted to different units. Mean discharge BMI was >19.0 in the CBTE groups, 16.0 in the short admission and 17.0 in the TAU groups.At one year follow up, 70% of patients receiving I-CBTE maintained good outcomes, in contrast with <5% TAU or planned short admissions. Readmission rates were 14.3% vs ~60% (χ2=0.0000). Standalone CBTE had intermediate outcomes. The main predictor of good long-term outcome was I-CBTE.ConclusionsOur main finding is that in a real-life setting, I-CBTE has superior short- and long-term outcomes as compared with alternative inpatient treatment models. This was achievable regardless of age, and BMI on admission. Dissemination of I-CBTE across the care pathway has the potential to transform outcomes of inpatient treatment for this high-risk patient population and reduce personal and societal costs.
Hydroxyapatite Adsorbed at the Surface of the Byssus Fibers of Pinna Nobilis
Authors: Giannossa Lorena C. , Mangone Annarosa , Lagioia Giovanni , Palazzo Gerardo , Gentile Luigi
Published: January 2022

Mussel-forming fibers manifest fascinating properties due to the numerous reactive functional groups present in their primary structure besides their secondary structures. Humans adopted these protein-based fibers to produce an extremely fine and valuable fabric, named byssus. Byssus is the bundle of filaments secreted by Pinna nobilis species employed to colonize the marine substrates. The byssus fibers coming from other species, such as Mytilus edulis, have been widely investigated in particular for their self-healing properties attributed to the collagen presence and their core-shell structure. Surprisingly, the Pinna nobilis byssus is non-collagenous based and the fibers nanostructure is still poorly understood. Here, we are reporting an investigation on the Pinna nobilis byssus samples retrieved intact in each stage of treatment. The scanning electron microscope (SEM) provides a peculiar feature of Pinna nobilis byssus, i.e. the elliptical cross-section of the fibers. Wide-angle X-ray scattering (WAXS), as well as infrared spectroscopy (IR), reveal the presence of hydroxyapatite adsorbed at the surface of the initial fibers and the presence of crystalline beta-sheet domains within the fibers. Finally, a WAXS-based method reveals a decrease in the crystalline/amorphous ratio during byssus treatment.
Association between the atherogenic index of plasma and major adverse cardiovascular events among non-diabetic hypertensive elderly patients
Authors: Hang Fei , Chen Jieruo , Wang Zefeng , Zheng Keyang , Wu Yongquan
Published: January 2022

Background: The literature on the association between the atherogenic index of plasma (AIP) with the risk of major adverse cardiovascular events (MACEs) among non-diabetic hypertensive elderly patients is quite limited at present. Methods: We performed a post-hoc analysis of data obtained from the Systolic Blood Pressure Intervention Trial (SPRINT) and explored the predictive value of AIP on the risk of MACEs among non-diabetic hypertensive elderly patients, to evaluate whether the benefit of intensive blood pressure control in preventing MACEs is stable in different AIP subgroups. Results: 9,323 participants with AIP were included in this analysis, and 561(6.02%) participants had composite cardiovascular outcomes during a median of 3.22 years of follow-up. The subjects in the highest quartile of AIP had a significantly increased risk of the primary outcome. In the fully adjusted Model 3, the adjusted HRs of the primary outcome for participants in Q2, Q3, and Q4 of AIP were 1.32 (1.02, 1.72), 1.38 (1.05, 1.81), and 1.56 (1.17, 2.08) respectively. Consistently, the trend test for the association between AIP quartiles and the primary outcome showed that a higher AIP quartile was associated with a significantly higher risk of the primary outcome (adjusted HR (95%CI) in Model 3: 1.14 (1.04, 1.25), P=0,004). However, within each AIP quartile, absolute event rates were lower for the intensive treatment group. No evidence was found for the interaction of intensive blood pressure control and AIP for the risk of the primary outcome (P for interaction = 0.932).Conclusion: This study found that elevated AIP is independently and positively associated with the risk of MACEs among non-diabetic elderly hypertensive patients. The benefit of intensive blood pressure control in managing cardiovascular events was consistent in different AIP subgroups.
Evaluation of a Modified Coagulation and Clipping Method To Prevent Postoperative Bleeding After Gastric Endoscopic Submucosal Dissection: A Multicenter Study
Authors: Terasaki Kei , Okuda Kotaro , Sakai Kyoko , Shirono Takao , Amioka Shohei , Nonaka Takahiro , Miura Takahiro , Iwai Naoto , Fujino Seiji , Oya Hirohisa , Katayama Takayuki , Miyawaki Kiichirou , Okuda Takashi , Matsumoto Junko , Mitsumoto Yasuhide , Mizuno Chiemi , Mizuno Masayuki , Mitsufuji Shoji , Shima Toshihide , Itoh Yoshito , Okanoue Takeshi
Published: January 2022

Postoperative bleeding is one of the most frequent adverse events in endoscopic submucosal dissection (ESD). Post-ESD coagulation (PEC) is widely performed to prevent postoperative bleeding; nonetheless, the postoperative bleeding rate after gastric ESD remains 3.1 - 5.5%. This study aimed to compare postoperative bleeding rates between patients receiving conventional treatment and those receiving the modified coagulation and clipping (MCC) treatment. Overall, 481 patients were treated between April 2007 and July 2020. The PEC method was perfomed from April 2007 to June 2010, whereas the MCC method was performed from July 2010 to July 2020. Clinicopathological characteristics were retrospectively compared between patients who underwent these two methods using univariate analysis and propensity score matching (PSM) analysis. Of the 481 included patients, 160 and 321 underwent PEC and MCC, respectively. Nine patients (5.6%) in the PEC group developed postoperative bleeding, whereas no patient (0.0%) in the MCC group developed postoperative bleeding. The univariate analysis and PSM analysis showed that the MCC method was significantly associated with the prevention of postoperative bleeding. In conclusion, the postoperative bleeding rate is lower with the MCC method than with the conventional method. The MCC method may be beneficial in preventing postoperative bleeding.
Promoter Motif And Conserved Module Analysis In DNA And RNA Using Almodules
Authors: Aydinli Muharrem , Liang Chunguang , Dandekar Thomas
Published: January 2022

Identification of transcription factor binding sites (TFBSs) elucidates regulation and control of transcription and gene expression. Overprediction of TFBS due to the high abundance of the small binding motifs is a notorious problem. For this comparative evaluation of TFBS is important.The tool AIModules presented in this paper presents an integrated solution for TFBS analysis as Web service and downloadable software. It works for other DNA and RNA motifs as well. Several sequences (DNA and RNA) are searched for TFBSs using predefined matrices from the JASPAR DB or by using own matrices for DNA or RNA motif discovery. Furthermore, AIModules can find TFBS common to two or more sequences. Demanding high or low conservation AIModules outperforms other solutions in speed and finds more modules (specific combinations of TFBS) than alternatives. The web application searches also RNA motifs (e.g. polyadenylation motif) as well as other DNA motifs (e.g. enhancers, silencers) and motif combinations. The application is free and open source to be used on-site or locally. These functionalities allow a number of biological applications, in particular analysis of promoters, including conserved modules and shared TFBSs in conserved gene families, presence of modules, motifs or regulatory elements in mRNA.
Artistic Gymnastics as a Semiotic Construct: The Case of the Chinese National Team at the Olympic Games
Authors: Wang Jiya , Yao Dawei
Published: January 2022

China is one of the most dynamically developing countries that experienced rapid and tumultuous modernization in the second half of the XX century. This modernization consisted of the sports organization improvement and appropriate target-setting in Olympic sports. Sport is the most important sphere of international communication, and gymnastics pertains to the expression of semiotic units by the movement itself. The purpose of this research is to study a semiotic construct embracing the victories, behavior patterns, and organization of the Chinese gymnastics team at the 2008 Olympic Games in Beijing, where China demonstrated its highest achievements in gymnastics. Another reason for selecting these Olympic Games is that semiotically significant elements of the athlete behavior differ from other contexts and Olympics. Consequently, the latter feature facilitates the segregation of these elements through phenomenological research.
Giant Adrenal Myelolipoma and Congenital Adrenal Hyperplasia: A Case Report and Review of the Literature
Authors: Chen Stephanie Yi-Tsi , Justo Monica AR , Ojukwu Kenechukwu , Russo Ashley , Balzer Bonnie L , Silberman Allan W , Gangi Alexandra
Published: January 2022

Purpose: Adrenal myelolipomas (AMs) are rare, benign tumors without endocrine activity comprised of mature adipose and hematopoietic tissue. Surgical excision may be required for lesions which have associated symptoms, those that are growing, or have features concerning for malignancy. Congenital adrenal hyperplasia (CAH) is often associated with adrenal masses such as AM. The majority of literature regarding AMs consists of case reports and case series with few describing giant AMs in the context of CAH. Case Report: We present the case of a 50-year-old woman with CAH and surgical history relevant for left adrenalectomy who presented to the emergency department with abdominal pain. Computed tomography (CT) imaging led to a diagnosis of diverticulitis which was treated non-operatively. Their scan also revealed a 20x13x23-cm right retroperitoneal mass causing mass effect on the duodenum, pancreas, right kidney, and inferior vena cava. CT-guided biopsy demonstrated adrenal and adipose tissue with hematolymphoid aggregates possibly representing myelolipoma but unable to rule out malignancy. Due to concern for retroperitoneal liposarcoma, the patient underwent an exploratory laparotomy, resection of >40-cm right retroperitoneal mass, right nephrectomy, right adrenalectomy, and cholecystectomy. Final pathology demonstrated a myelolipoma with intra-tumoral ossification and hemorrhage as well as diffuse cortical hyperplasia with lymphoid aggregates. Due to acquired complete adrenal insufficiency, the patient required stress-dose steroids and steroid taper post-operatively.Conclusion: Key points from this report include the occurrence of a giant AM in the context of CAH and special considerations in the diagnosis and operative approach of a giant retroperitoneal mass.
Self-employment and health inequality of migrant workers
Authors: Wen xin , Zhou deshui
Published: January 2022

Objective: This research aims to discuss the impact of self-employment on health inequality of migrant workers, and explore the mechanism and group differences of the influence of self-employment on health inequality in Chinese migrant workers.Background: Self-employment is one of the main types of employment for migrant workers in China, it is important to promote self-employment of migrant workers for Chinese employment-first policy. However, self-employment will affect the life and behavior of migrant workers, as well as health inequality of them.Method: This research uses the data from the 2018 China Migrant Workers Dynamic Monitoring Survey, and examines the impact of self-employment on health inequality of migrant workers by using RIF-I-OLS decomposition method.Results: We find that self-employment will reduce the health inequality of Chinese migrant workers significantly, especially among migrant workers with low education, low income groups and low social integration. Further analysis shows that self-employment can directly promote the self-rated health of migrant workers. Moreover, it indirectly alleviates the health inequality of migrant workers by mediating effect of improving public welfare accessibility such as health records and health education.Conclusion: It is necessary to allow and support migrant workers to engage in self-employment activities, the government should provide public services such as health education, health records and health rights for migrant workers, focus on the employment of migrant workers in city, especially those with low income and low education. Therefore, we believe that measures should be taken to enhance the sense of belonging of migrant workers in city, and promote migrant workers to truly achieve “urbanization of people”. In this manner, reducing the health inequality of migrant workers.
Pneumonia and COVID-19 Classification in Chest X-rays Using Faster Region-Based Convolutional Neural Networks (Faster R-CNN)
Authors: Farhat Hanan J , Sakr Georges E. , Kilany Rima , Smayra Tarek , Mallak Iyad
Published: January 2022

Chest X-rays have been a subject of deep learning applications on medical images forthe high expectations of their evidence in assessing pulmonary diseases. Whereas thearising of COVID-19 or 2019 novel coronavirus in December 2019 have prioritizedresearch on pulmonary diseases diagnosis and prognosis, especially using artificialintelligence (AI) and Deep Learning (DL). For this sake, we extend the work ondetecting pneumonia using Faster Region-Based Convolutional Neural Networks(Faster R-CNN) by applying Faster R-CNN to the detection of Pneumonia and COVID-19 in Chest X-ray images using several datasets involving COVID-19 images. Differentcombinations of training scenarios in addition to internal and external testing atdifferent objectiveness thresholds, epochs counts, and lengths yielded variant results.Our results comply with the state of the art of Faster-RCNN in pneumonia detection butdo not show promising results in COVID-19 detection as a standalone model. Futurework may emphasize introducing segmentation to the model pipeline, adding asecondary classification stage, and even engaging other medical data to improve theperformance and constitute a robust Faster R-CNN-based prediction model.
Complete Genome Sequence of Aconitum Amalgavirus 1, a Distinct Member of the Genus Amalgavirus
Authors: Li Fan , Yang Jie , Lan Ping-Xiu , Li Jin-Ming , Chen Xiao-Jiao , Tan Guan-Lin , Wei Tai-Yun , Li Ru-Hui
Published: January 2022

A novel virus named Aconitum amalgavirus 1 (AcoAV-1) was identified from Chinese aconite (Aconitum carmichaelii) plants with viral disease symptoms. The genome of this monopartite dsRNA virus is 3,370 nucleotides long, which containing two partially overlapping open reading frames encoding a putative coat protein and RNA dependent RNA polymerase, respectively. AcoAV-1 shares 34.9-50.7% amino acid sequence identities to other amalgaviruses. Phylogenetic analyses group AcoAV-1 with blueberry latent virus and other related viruses in a clade, suggesting that it belongs to the genus Amalgavirus in the family Amalgaviridae.
The ratio of CD8+ lymphocytes to tumor-infiltrating suppressive FOXP3+ effector regulatory T cells is associated with treatment response in invasive breast cancer
Authors: Kadoya Takayuki , Goda Noriko , Sasada Shinsuke , Shigematsu Hideo , Masumoto Norio , Arihiro Koji , Nishikawa Hiroyoshi , Sakaguchi Shimon , Okada Morihito
Published: January 2022

Purpose: FOXP3+ tumor-infiltrating lymphocytes (TILs) are prototypical immunosuppressive TILs in breast cancer. However, the FOXP3+ TIL subset of effector regulatory T cells (eTregs) cannot be detected with conventional immunohistochemical staining. Therefore, we evaluate the immunosuppressive potential of eTregs and the role of immunostimulatory CD8+ TILs in invasive breast cancer.Methods: Fresh TILs were extracted from 84 patients with breast cancer and divided into CD4+FOXP3+ regulatory T cells (Tregs) and CD8+ cytotoxic T lymphocytes (CTLs) by flow cytometry. Tregs were further classified into eTregs (CD4+FOXP3highCD45RA−), other FOXP3+ Treg subsets (naïve and non-Tregs), and total CD8+CD4- TILs.). The association between TILs subpopulation, clinicopathological characteristics, and the response to chemotherapy was evaluated.Results: The median eTreg proportion among total CD4+ TILs was 18.7% (interquartile range [IQR], 16.4–25.5%), whereas this proportion for CD8+TILs was 124% (IQR, 87.5–140%). The proportion of eTregs to total FOXP3+ TILs varied widely among patients (median, 65.6%; range, 10.1–93.2%). In an immunosuppression assay with Treg subsets, only eTregs displayed potent immunosuppression. Among the patients who received neoadjuvant chemotherapy (NAC), the pathological complete response(pCR) rate was significantly higher among individuals with a high CD8+/eTreg ratio (90.2% vs. 33.3%). TILs predominance and CD8/eTreg ratio were independent predictive factors for pCR to chemotherapy (P = 0.043 and P = 0.034, respectively). Conclusions: The CD8+/eTreg ratio is a simple, optimal indicator of cancer immunity, and a high CD8+/eTreg ratio may enhance the prognosis and treatment response of patients with invasive breast cancer. Further studies are warranted to validate the present findings.
Factor XII silencing using siRNA prevents thrombus formation in a rat model of extracorporeal life support
Authors: Umei Nao , Shin Suji , Lai Angela , Miller Jennifer , Roberts Kalliope , Strelkova Daria , Chaudhary Namit , Ichiba Shingo , Sakamoto Atsuhiro , Whitehead Kathryn , Cook Keith
Published: January 2022

Background: Heparin anticoagulation increases the bleeding risk during extracorporeal life support (ECLS) and thus must be limited and monitored carefully. This study determined whether factor XII (FXII) silencing using short interfering RNA (siRNA) can provide ECLS circuit anticoagulation, maintain normal tissue coagulation, and reduce the need for careful anticoagulation monitoring.Methods: Adult male, Sprague-Dawley rats were randomized to four groups (n = 3 each) based on anticoagulant: (1) no anticoagulant, (2) heparin, (3) FXII siRNA, or (4) non-targeting siRNA. In group 1, no anticoagulant was administered. In group 2, heparin was administered intravenously before and during ECLS at an activated clotting time of 180–250 seconds. In groups 3 and 4, siRNA was administered intravenously three days before the initiation of ECLS via lipidoid nanoparticles. The rats were placed on pumped, arteriovenous ECLS with a small 3D-printed mock-oxygenator, and blood flow was maintained at 2 mL/min for eight hours or until the blood flow resistance reached three times its baseline resistance.Results: A single dose of 3 mg/kg siRNA led to a reduction of plasma FXII to 26% ± 3% (mean +/- standard error) of baseline levels three days after treatment. Without anticoagulant, mock-oxygenator resistance tripled within 7 ± 2 minutes. The resistance in the heparin group was significantly (p < 0.05) elevated in the first hour of the experiment before returning to a lower, normal value for the rest of the experiment. The resistance in the FXII siRNA group did not change significantly with time (p = 0.94). There were no significant differences in resistance or mock-oxygenator thrombus volume between the FXII siRNA and the heparin groups (p > 0.99). However, the bleeding time in the FXII siRNA group (3.4 ± 0.6 minutes) was significantly shorter than that in the heparin group (5.5 ± 0.5 minutes, p < 0.05).Conclusions: FXII silencing using siRNA provided simpler anticoagulation of ECLS circuits with reduced bleeding risk as compared to heparin.
Infectious viral load in unvaccinated and vaccinated patients infected with SARS-CoV-2 WT, Delta and Omicron
Authors: Meyer Benjamin , Puhach Olha , Adea Kenneth , Hulo Nicolas , Sattonnet Pascale , Genecand Camille , Iten Anne , Bausch Frédérique Jacquérioz , Kaiser Laurent , Vetter Pauline , Eckerle Isabella
Published: January 2022

Viral load (VL) is one determinant of secondary transmission of SARS-CoV-2. Emergence of variants of concerns (VOC) Alpha and Delta was ascribed, at least partly, to higher VL. Furthermore, with parts of the population vaccinated, knowledge on VL in vaccine-breakthrough infections is crucial. As RNA VL is only a weak proxy for infectiousness, studies on infectious virus presence by cell culture isolation are of importance.
We assessed nasopharyngeal swabs of COVID-19 patients for quantitative infectious viral titres (IVT) by focus-forming assay and compared to overall virus isolation success and RNA genome copies. We assessed IVTs during the first 5 symptomatic days in a total of 440 patients: unvaccinated individuals infected with pre-VOC SARS-CoV-2 (n= 118) or Delta (n= 127) and vaccine-breakthrough infections with Delta (n= 133) or Omicron (n=62). Correlation between RNA copy number and IVT was low for all groups. No correlation between IVTs and age or sex was seen. We observed higher RNA genome copies in pre-VOC SARS-CoV-2 compared to Delta, but significantly higher IVTs in Delta infected individuals. Vaccinated Delta infected individuals had significantly lower RNA genome copies and IVTs compared to unvaccinated subjects and cleared virus faster. In addition, vaccinated individuals with Omicron infection had lower IVTs in comparison to Delta breakthrough infections.
Quantitative IVTs can give detailed insights into virus shedding kinetics. Vaccination was associated with lower infectious titres and faster clearance for Delta, showing that vaccination would also lower transmission risk. Omicron vaccine-breakthrough infections did not show elevated IVTs compared to Delta, suggesting that other mechanisms than increased VL contribute to the high infectiousness of Omicron.
Gargle and mouthwash can replace nasopharyngeal swab sampling when concentrated with a new method for diagnosis of COVID-19
Authors: Sancak Banu , Mutlu Esvet , Cirit Osman Sezer , Kocagöz Tanil , Can Özge , Çicek Candan , Sayiner Ayça Arzu , Appak Özgür , Uyar Neval Yurttutan , Külah Canan , Çiçek Aysegül Çopur , Özgümüs Osman Birol , Altintop Yasemin Ay , Saatçi Esma , Karsligil Tekin , Zer Yasemin , Özen Nevgün Sepin , Çekin Yesim , Karahan Zeynep Ceren , Evren Ebru , Karakoç Ayse Esra , Gülbahçe Sultan , Mutlu Derya , Bozdemir Tugba , Çayci Yeliz Tanriverdi , Çinar Canberk , Tasbakan Meltem , Mert Merve , Çinar Ece , Kutsoylu Oya Özlem Eren , Kocagöz Sesin , Ertürk Ayse , Çelik Ilhami , Mete Ayse Özlem , Eneyli Müge Günalp , Akdemir Irem , Karakök Taliha , Inan Dilara , Atilla Aynur
Published: January 2022

Diagnosis of COVID19 is based on reverse transcription-polymerase chain reaction (RT-PCR) testing of nasopharyngeal swab (NPS) samples. As NPS sampling is a discomforting invasive procedure, studies were conducted to investigate the performance of saliva and mouthwash (MW) samples in RT-PCR. There is limited data about MW samples. We have developed a new method for concentrating gargle and mouthwash (GMW) samples to be used in RT-PCR. In our study, we aimed to investigate the performance of concentration of GMW samples in detection of SARS-CoV-2. A paired sample of NPS and GMW samples were collected from patients in 11 centers in Turkey. MW samples were concentrated using MyMagiCon-RW100® (Bio-T, Istanbul, Turkey). NPS, GMW and concentrated GMW samples were tested by RT-PCR for the presence of SARS-CoV2 and the results were compared. The viral RNA was detected in 47.5% of NPS samples, in 28.8% of GMW before concentration and in 37.5% GMW samples after concentration. Concentration of samples increased the number of samples in which SARS-CoV2 RNA was detected by 16.6%. The RT-PCR of concentrated GMW samples yielded better results than the RT-PCR of NPS samples in two centers. Concentrated GMW sampling can be an alternative method to NPS sampling in rapid and accurate diagnosis of COVID-19.
Weighted Gene Correlation Network Analysis (WGCNA) of Arabidopsis somatic embryogenesis (SE) and identification of key gene modules to uncover SE-associated hub genes
Authors: de Silva Kithmee K. , Dunwell Jim M. , Wickramasuriya Anushka M.
Published: January 2022

Background

Somatic embryogenesis (SE), which occurs naturally in many plant species, serves as a model to elucidate cellular and molecular mechanisms of embryo patterning in plants. Decoding the regulatory landscape of SE is essential for its further application. Hence, the present study aimed to employ Weighted Gene Correlation Network Analysis (WGCNA) to construct a gene co-expression network (GCN) for Arabidopsis SE, and then identify highly correlated gene modules to uncover the hub genes associated with SE that may serve as potential molecular targets.
Results

A total of 17,059 genes with a variance greater than 0.25 quantile were filtered from microarray datasets derived from Arabidopsis SE (NCBI Gene Expression Omnibus accession number: GSE48915). This included 1,711 transcription factors and 445 EMBRYO DEFECTIVE genes. GCN analysis identified a total of 26 gene modules with the module size ranging from 35 to 3,418 genes using a dynamic cut tree algorithm. The module-trait analysis revealed that four, four, seven and four modules were associated with Stage I (zygotic embryos), Stage II (proliferating tissues at 7 days of induction), Stage III (proliferating tissues at 14 days of induction) and Stage IV (mature somatic embryos), respectively. Further, we identified a total of 260 hub genes based on the degree of intramodular connectivity. Validation of the hub genes using publicly available expression datasets demonstrated that at least 78 hub genes are potentially associated with embryogenesis; of these many genes remain functionally uncharacterized thus far. In silico promoter analysis of these genes revealed the presence of EBOXBNNAPA and SEF4MOTIFGM7S motifs; this suggests these genes may play important roles in plant embryo development.
Conclusion

The present study successfully applied WGCNA to construct a GCN for SE in Arabidopsis and identified hub genes involved in the development of somatic embryos. These hub genes could be used as molecular targets to further elucidate the molecular mechanisms underlying SE in plants.
Insulin Resistance a Dysfunction far Beyond the Beta Cell
Authors: Andrade Luis Jesuino de Oliveira , de Oliveira Luís Matos , Bittencourt Alcina Maria Vinhaes , de Oliveira Gabriela Correia Matos
Published: January 2022

Insulin resistance (IR) is recognized as a biological reaction to insulin stimulation in target tissues. IR alters glucose metabolism, resulting in an elevation in insulin production by beta-cells. The main condition that accompanies IR in our environment is obesity due to environmental factors, especially diet, which over the years has gradually taken hold in our civilization. Objective: To describe the IR in different organs and present the signaling pathway project. Methods: PubMed database was employed to search IR review publications. The referenced data of the signaling pathway was chosen by aggregating references from the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A signaling pathway was designed based on the IR research manuscripts, where we show various mechanisms involved. The KEGG server was employed to explore the protein-protein interrelationship, and devise the signaling pathway diagram. The mapping of the signaling path was performed with the PathVisio software, adapting to the model of KEGG PATHWAY Database: https://www.genome.jp/pathway/map04930. Results: We selected articles from the PubMed database that featured the terms "insulin resistance" and "signaling pathway." Based on research articles validated by the database, we chose well-founded pathways and achieved a representative description of these pathways. Reproduction contigs taken from the KEGG database projected the signaling pathway of biomolecules leading to IR. Thus, the action between multiple mechanisms releases factors that participate in the development of IR. Conclusion: Interaction between multiple mechanisms and molecular interactions are important factors in development of IR in various organs and systems.
Electrolytic and hemogasometric parameters for female agoutis (Dasyprocta prymnolopha Wagler, 1831) raised in captivity
Authors: Muniz Marta Silva , Carreiro Artur da Nóbrega , Albuquerque Erica Emerenciano , Diniz João Augusto Rodrigues Alves , Souza Joyce Galvão de , Menezes Danilo José Ayres de , Vaz Antônio Fernando de Melo
Published: January 2022

Agoutis (Dasyprocta prymnolopha, WAGLER 1831) have been studied in captivity as a potential source of meat for rural populations in Brazil, as a way of providing low-cost protein and easing hunting pressures in local communities. However, little is known about their physiological blood markers, which are crucial for animal husbandry and effective welfare management. This study provides hemogasometric and ionic venous parameters for female agoutis raised in captivity in the drylands of Brazil. Venous blood samples were collected from six females and analyzed for dissolved gases and ions in the blood. Hemogasometric parameters, especially base excess and anion gap were generally heterogenous, while ions varied little among individuals. Moreover, we observed high chlorine values and negative base excess values, which suggest that some animals might be implementing compensatory mechanisms for ion imbalances. We provide a preliminary note on agouti venous gases and ions, while reiterating the importance of the clinical context and in-house parameter definition to maximize accuracy.
Bilateral inflammatory aural polyps in a kitten – case report
Authors: Botelho Cristiane Bazaga , Sampaio Rafaella Tortoriello Barbosa , Lopes Natália Lôres , Fernandes Julio Israel
Published: January 2022

Feline aural inflammatory polyps are non-neoplastic masses in the tympanic cavity or the Eustachian tube that can be observed in the ear canal of the cat and are a common cause of otitis externa and otitis media in young animals, with or without respiratory signs depending on the direction of polyp growth. Most of the polyps occurring in cats are unilateral. Otoscopy or video-otoscopy is necessary for its diagnosis, and treatment consists of the mechanical removal of the polyp and subsequent use of anti-inflammatory drugs. The aim of this paper was to report a case of bilateral inflammatory aural polyps in a kitten treated successfully by the minimally invasive technique of per-endoscopic trans-tympanic traction. A seven-month-old female mixed-breed cat, presenting bilateral ear discharge, was submitted to endoscopic examination of both ears and polypoid masses observed in each horizontal canal. Both polyps were grasped and pulled with a polypectomy snare through the otoendoscope. The patient received dexamethasone immediately after the procedure and a long-term oral corticosteroid protocol was established post-avulsion. It was concluded that otoendoscopy allowed an accurate and quick diagnosis, that the mass removal by per-endoscopic trans-tympanic traction was an effective treatment with no complications, and that bilateral occurrences could indicate a differential diagnosis, especially in young cats.
Association of porcine circovirus infection with mycotoxicosis: a case report
Authors: Araújo Lina Raquel Santos , Silva Ênio Campos da , Andrade Tiago Silva , Viana Neto Aderson Martins , Evangelista José Nailton Bezerra , Matos Amanda Veruska Silva de
Published: January 2022

Circovirus is widespread in pig farming, and mainly affects piglets increasing morbidity and mortality rates, being vaccination the most effective strategy to control one. However, for an effective vaccine response, certain factors must be considered, such as the adoption of good practices during the conservation and handling of vaccines, environmental challenges, nutritional and health status of the animals, and presence of immunosuppressive agents, such as mycotoxins, in the feed. Here, we describe a circovirus outbreak associated with mycotoxin immunosuppression that occurred in the piglets during the nursery phase at a commercial farm, which initiated with a sudden increase in the mortality of vaccinated piglets. Blood samples were collected and analyzed using RT-PCR, while the feed was subjected to mycotoxicological analysis. RT-PCR analysis revealed the presence of porcine circovirus type 2 (PCV-2) in the blood serum samples, thereby confirming the circovirus outbreak. The feed analysis revealed elevated levels of mycotoxins (deoxynivalenol, aflatoxins, and fumonisins), which were above the levels tolerated by the piglets during the nursery phase. Therefore, the contaminated feed was discarded, and a new ration was made available. Concurrently, the vaccination program was amended to normalize the mortality rate. The presence of mycotoxins in the feed could be the predisposed factor for piglet infection caused by PCV-2 and other diseases. This is an important aspect because the immunosuppressive effect of mycotoxins can alter the vaccine response, thereby making the piglets more susceptible to the diseases even after being vaccinated, although they should be immunologically protected.
Analgesic Efficacy and Safety of Spinal Oxycodone in Total Hip
Arthroplasty: A Preliminary Study
Authors: Kowalski Grzegorz , Olczak Bogumił , Leppert Wojciech , Bienert Agnieszka , Teżyk Artur , Szkutnik-Fiedler Danuta , Adamski Michał , Wieczorowska-Tobis Katarzyna
Published: January 2022

Aim:

The aim of this study was to assess the analgesic efficacy and safety of 1
mg and 0.5 mg oxycodone administration in a spinal block procedure for a total hip
arthroplasty (THA).

Patients and Methods:

Forty-two THA patients aged 59-81 with American Society Anesthesiology
(ASA) II-III were included. All patients received anesthesia using spinal
blockade, with bupivacaine 0.5% spinal heavy 2.5 ml, with 0.5 ml oxycodone hydrochloride
1.0 mg (group A; n = 28) or 0.5 mg (group B; n = 14). During surgery, each patient
was sedated with 2-4 mg/kg/h intravenous propofol infusion. They received 100 mg intravenous
ketoprofen at the end of the surgery at 8 pm and 8 am, with recommended doses
every 12 h thereafter. Subcutaneous morphine 5 mg was used as a rescue analgesic, and
the time to morphine use was recorded. After surgery, pain intensity (at the moment of
patient report) was assessed using an 11-point numerical rating scale (NRS). The incidence
of adverse effects was monitored. Blood samples were taken for assays of serum
oxycodone, noroxycodone and bupivacaine levels.

Results:

The time to rescue analgesia was 9.6 ± 5.6 h in group A and 7.3 ± 1.9 h in group
B, and it did not differ between patient groups (P = 0.179). The mean NRS pain score
was 4.5 in group A and 4.2 in group B. Three group A patients had detectable oxycodone
levels: two < 7.1 ng/ml and in 1 spinal block induced anesthesia was unsuccessful and so
he/she underwent general anesthesia (this patient was excluded from the analysis). Four
group B patients had single values < 5 ng/ml. Noroxycodone levels were in all patients
undetectable, and bupivacaine levels were 70-300 ng/ml. Regarding adverse effects, one
patient had hypotension, one had bradycardia, and one had pruritus.

Conclusion:

Oxycodone in spinal block prolongs analgesia period, does not cause serious
adverse effects and seems to be safe and effective opioid for patients undergoing
THA.
Versatile Approach for the Synthesis of Furo-coumarin Derivatives
Authors: Singh Nidhi , Rajotiya Krishna , Lamba Nikita , Singh H.L. , Ameta K.L. , Singh Shivendra
Published: January 2022

Owing to useful physio-chemical properties of furo-coumarin derivatives, their
synthetic and mechanistic investigation has been reported here. We have demonstrated a
range of synthetic approaches to access furan-fused coumarin derivatives. Many metalmediated,
base, and acid-catalyzed approaches have been revealed for the formation of this
coumarin-based fused heterocycles of biological importance. In addition to this, microwaveassisted
synthetic routes have also been revealed. The last and most useful approach for the
synthesis of these heterocycles includes the use of purely solvent as a reaction media for synthesizing
these interesting classes of heterocycles.
Recent Advances in Utilization of Deep Eutectic Solvents: An Environmentally Friendly Pathway for Multi-component Synthesis
Authors: Thakur Ajay , Verma Monika , Bharti Ruchi , Sharma Renu
Published: January 2022

With the increasing analysis of saving the environment, the researchers demonstrated much effort to replace toxic chemicals with environmentally benign ones. Eutectic mixtures are those solvents that fulfill the criteria of green solvents. The synthesis of organic compounds in the chemical and pharmaceutical industries makes it necessary to find unconventional solvents that cause no harmful impact on health parameters. This review showed that using deep eutectic mixture-based solvents to overcome the hazardous effects of harmful volatile organic solvents over the past few years has gained much more appeal. In most applications, deep eutectic mixtures are used for a solvent or co-solvent role, as they are easy to use, easy dissolution of reactants, and non-evaporative nature. However, deep eutectic mixtures have also been investigated as catalysts, and this dual functionality has much scope in the future, as a significantly less range of deep eutectic mixtures is utilized for this.
Acute Myocardial Infarction in Hemophilia A with HIV and Hyperhomocysteinemia-
A Rare Case Report
Authors: Rohila Amit Kumar , Kachhwaha Arjun , Tripathi Swapnil , Meena Durga Shankar , Khichar Satyendra , Kumar Deepak
Published: January 2022

Background:

Patients with hemophilia have a hypocoagulable state and less chances of
thrombus formation. Therefore, they are expected to have a lower cardiovascular mortality than the
general population. The lower cardiovascular mortality can be explained by less chances of thrombus
formation due to hypocoagulability.

Case Presentation:

Here, we present a case of a 42-year-old male patient who presented with severe
chest pain radiating to the back for 4 days. ECG was suggestive of recent acute anteroseptal
myocardial infarction. There was a history of receiving intravenous recombinant factor VIII, 1
hour prior to the onset of chest pain for knee joint swelling. The occurrence of acute coronary syndromes
in patients with hemophilia A is uncommon and rarely reported.

Conclusion:

Here, we report a patient with severe hemophilia A who developed acute myocardial
infarction after administration of recombinant factor VIII.
Role of Long Non-coding RNAs in the Pathogenesis of Alzheimer’s and
Parkinson’s Diseases
Authors: Sivagurunathan Narmadhaa , Ambatt Aghil T.S. , Calivarathan Latchoumycandane
Published: January 2022

Neurodegenerative diseases are a diverse group of diseases that are now one of the leading
causes of morbidity in the elderly population. These diseases include Alzheimer’s disease
(AD), Parkinson’s disease (PD), Huntington’s disease (HD), Amyotrophic Lateral Sclerosis (ALS),
etc. Although these diseases have a common characteristic feature of progressive neuronal loss
from various parts of the brain, they differ in the clinical symptoms and risk factors, leading to the
development and progression of the diseases. AD is a neurological condition that leads to dementia
and cognitive decline due to neuronal cell death in the brain, whereas PD is a movement disorder
affecting neuro-motor function and develops due to the death of the dopaminergic neurons in the
brain, resulting in decreased dopamine levels. Currently, the only treatment available for these neurodegenerative
diseases involves reducing the rate of progression of neuronal loss. This necessitates
the development of efficient early biomarkers and effective therapies for these diseases. Long
non-coding RNAs (LncRNAs) belong to a large family of non-coding transcripts with a minimum
length of 200 nucleotides. They are implied to be involved in the development of the brain, a variety
of diseases, and epigenetic, transcriptional, and posttranscriptional levels of gene regulation. Aberrant
expression of lncRNAs in the CNS is considered to play a major role in the development and
progression of AD and PD, two of the most leading causes of morbidity among elderly populations.
In this mini-review, we discuss the role of various long non-coding RNAs in neurodegenerative
diseases, such as Alzheimer’s and Parkinson’s disease, which can further be studied for the development
of potential biomarkers and therapeutic targets for various neurodegenerative diseases.
Theoretical Study of HFO1336mzz(Z)/HFO1224yd(Z) Refrigeran t
Mixtures for High-temperature Heat Pump
Authors: Wang Zele , Zhang Hua , Huang Jie , Yang Meng , Wang Zilong
Published: January 2022

Background:

The global energy consumption problem is becoming more serious, therefore,
it is a hot trend to improve energy efficiency and choose environmentally friendly alternative working
fluids.

Objective:

The main thermophysical parameters of the refrigerant mixture, which blended HCFO
1224yd(Z) and HFO1336mzz(Z) in the high-temperature heat pump cycle, were theoretically studied to
replace HFC245fa. The theoretical cycle model of refrigerant mixtures of high-temperature heat pumps
was established.

Methods:

In this paper, a theoretical cycle model of the mixed refrigerant high-temperature heat pump
is established, and the cycle performance of the mixed refrigerant in the high-temperature heat pump
system under different working conditions is compared.

Results:

It was found that HFO1336mzz(Z) could reduce the exhaust temperature of the compressor and
increase the heat pump coefficient COPh, and HCFO1224yd(Z) could improve the problem of excessive
suction specific volume in high-temperature heat pump systems.

Conclusion:

The result shows that the refrigerant mixture with 0.6/0.4 HCFO1224yd(Z)/HFO1336
mzz(Z) has the best cycle performance when the condensing temperature is 150 °C, and the cycle temperature
rise ranges from 50 to 70°C.When the cycle temperature rise is 70°C, and the condensing temperature
ranges from 110 °C to 150°C, the coefficient of performance value of the refrigerant mixture
with 0.2/0.8 HCFO1224yd(Z)/HFO1336mzz(Z) reaches the maximum of 3.11.
effectiveness of developing gymnastics learning media with the application of teileren and global (ganze) a method based on lectora digital
Authors: Zulbahri Zulbahri , Astuti Yuni , Sasmitha Weny , Pitnawati Pitnawati , Erianti Erianti , Damrah Damrah , Rosmawati Rosmawati
Published: January 2022

This research was motivated by the limited media for sports learning Gymnastics material, especially for schools located in the regions. This research aims to produce digital learning media products in the development of teileren and global (Ganze) methods. This research was carried out by using “Research and Development” The model that is used as a reference in this research is the Borg & Gall development research with 10 developments, in this research, the researchers adopted the Steps as follows: 1) Preliminary Study; 2) Develop planning and analyze the media to be made; 3) product  Initial development; 4) Conduct small group trials; 5) Product Revision; 6) Conduct field trials; and 7) Revise the final product. The sample in this research were students of the PO FIK UNP Department who attended basic Gymnastics and learning lectures with a total of ninety people for small group subject trials and one hundred and forty people for field trials. As a result and conclusion of  this research, namely the resulting learning media products for practical material with a score of 4 with good criteria and media with a score of 4.33 with a very good category.
Детектор терагерцового излучения на основе термоэлектрического материала Bi-=SUB=-88-=/SUB=-Sb-=SUB=-12-=/SUB=--=SUP=-*-=/SUP=-
Authors: Макарова Е.С. , Асач А.В. , Тхоржевский И.Л. , Сединин А.Д. , Зыков Д.В. , Зайцев А.Д. , Демченко П.C. , Новоселов М.Г. , Комаров В.А. , Тукмакова А.С. , Новотельнова А.В. , Каблукова Н.С. , Ходзицкий М.К.
Published: January 2022

Terahertz radiation is very promising for visualization, detection and data transfer. Searching for sensitive, fast and compact THz detector that operates at room temperature is a common subject for these applications. Hence, there is still an issue of searching for new materials for THz radiation detection.
Solid solutions based on thermoelectric bismuth and antimony appear to have significant potential for these applications. In this paper photoresponse of thermoelectric material Bi88Sb12 has been
studied for the first time. Films with thicknesses of 70 and 150 nm were studied under influence of radiation at frequency of 0.14 THz.
Синтез и свойства коллоидных квантовых точек селенида ртути
Authors: Жуков Н.Д. , Цветкова О.Ю. , Гавриков М.В. , Роках А.Г. , Смирнова Т.Д. , Штыков С.Н.
Published: January 2022

Colloidal nanocrystals (quantum dots (QDs)) of mercury selenide have been synthesized and the effect of size quantization on their basic properties has been investigated. The current-voltage characteristics of single QDs (less than 10 nm) had features in the form of separate regular peaks and quasi-periodic current oscillations with voltage intervals (0.1–0.2) V. The observed features were explained in the models of size quantization and Bloch oscillations. The absorption spectra in the range up to 25 μm had eight certain peaks, including five – interband and intraband transitions and three – with energies (145–215) meV, which are explained as intraresonant. Calculations show that it is possible to have IR photosensitivity in the wavelength range up to 40 μm.