bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy of nintedanib as a host-directed therapy candidate in the treatment of tuberculosis
Authors: Li Xinda , Qi Xueting , Wang Bin , Fu Lei , Chen Xi , Luo Xiaoyi , Chen Xiaoyou , Lu Yu
Published: December 2024

Background

The lengthy duration and high frequency of drug resistance associated with currently used antimycobacterial drug treatments have intensified the need for alternative therapies against Mycobacterium tuberculosis, the causative agent of TB.

Methods

MICs and intracellular macrophage cfu counts were tested to evaluate the antibacterial activity of nintedanib and pirfenidone against drug-susceptible and -resistant M. tuberculosis. A chronic murine model of pulmonary infection was used to assay the therapeutic efficacy of nintedanib. Macrophage transcriptome deep sequencing, a confocal assay, siRNA knockdown, Western blotting, quantitative RT–PCR and a cfu assay were used to investigate the antibacterial mechanism of nintedanib.

Results

The MIC90 of nintedanib against M. tuberculosis standard strain H37Rv was 23.56–40.51 mg/L. TB murine model studies showed that nintedanib, coadministered with isoniazid, rifampicin and pyrazinamide, shortened treatment duration, and ameliorated pulmonary inflammation and fibrosis. In mechanism studies, transcriptome sequencing analysis revealed that nintedanib may eliminate M. tuberculosis through up-regulating macrophage autophagy. Furthermore, inhibition of autophagy by using siRNA targeting ATG5 or the autophagy inhibitor 3-methyladenine almost completely abolished nintedanib-mediated suppression of M. tuberculosis. Nintedanib induced autophagy by the JAK2/STAT3/Beclin1 pathway. When JAK2 or Beclin1 were knocked down through siRNA, nintedanib no longer inhibited M. tuberculosis. JAK2 activator coumermycin A1 and STAT3 agonist colivelin also reversed this phenotype.

Conclusions

In vitro activity of nintedanib against drug-susceptible and -resistant M. tuberculosis and efficacy in murine infections warrant the continued clinical evaluation of nintedanib as a new adjuvant therapy for standard treatment of TB.
Effects of plant crown characteristics on dispersal kernels of wind-dispersed seeds under low wind speeds
Authors: Qin Xuanping , Liang Wei , Liu Minghu , Xin Zhiming , Wang Zhigang , Zhou Quanlai , Tian Liang , Zong Lu , Zhu Jinlei , Liu Zhimin
Published: December 2024

Plant crown can affect the seed dispersal process. Clarifying the influence of plant crown type on seed dispersal kernel is important for predicting species distribution. However, the impact of different plant crown types on seed dispersal has rarely been tested. To determine the effect of plant crown type on seed dispersal kernel, we conducted wind tunnel experiments in which we investigated seed dispersal average distance, range, kurtosis and skewness of 29 species with different diaspore traits (type of appendage, mass, projected area, shape index, wing loading and terminal velocity) under 3 wind speeds (2, 4 and 6 m•s-1) for seed passing through different crown types (classified based on crown size, branch density and with or without leaves). We fit functions to the seed dispersal distance and found that the Gaussian function had the best fit. Plant crown type had significant effects on dispersal kurtosis and skewness, but not on dispersal average distance and range, of seeds passing through it under low wind speed condition. Width and branch density of plant crown affected dispersal kurtosis, and leaves of plant crown influenced kurtosis and skewness. Increase in wind speed reduced the influence of planting crown on the dispersal kurtosis and skewness. Plant crown had no significant effect on average seed dispersal distance but affected seed dispersal kernel shape, so, the dispersal range of seeds through different plants is invariant while the density of dispersal varies greatly. The information could be helpful for understanding plant metapopulation dynamics.
Having more resilience factors only gets you so far in the context of neighborhood disadvantage: understanding differential predictors of internalizing symptoms and body mass index in youth with spina bifida
Authors: Scott Samantha Ruby , Peterson Robin L , North Samantha J , Merrick Jillian Sari , Petranovich Christine L , Wilson Pamela E , Lantagne Ann , Simpson Tess S
Published: December 2024

Objective

The primary aim of this study was to determine if neighborhood disadvantage predicted internalizing symptoms and body mass index (BMI) in youth with spina bifida (SB), while accounting for several sociodemographic factors. We also explored whether resilience factors helped explain associations between neighborhood disadvantage and internalizing symptoms or neighborhood disadvantage and BMI.

Methods

Participants (n = 69, Mage=14.13, Range: 8–20, 51% biological female, 25% Hispanic/Latinx White, 28% Other/Multiracial, 48% non-Hispanic/Latinx White) were drawn from a retrospective chart review of clinical data, including self-reported resilience factors and internalizing symptoms, collected as part of the standard of care in a multidisciplinary pediatric SB clinic. Neighborhood disadvantage scores were extracted from participant addresses. Using structural equation modeling, we examined whether neighborhood disadvantage predicted BMI and internalizing symptoms. We explored whether these paths were mediated by resilience factors.

Results

Higher neighborhood disadvantage predicted higher BMI and lower internalizing symptoms. Higher neighborhood disadvantage predicted higher resilience factors, which predicted lower internalizing symptoms. Higher resilience factors did not predict BMI.

Conclusions

Neighborhood disadvantage may require youth with SB to navigate their way to additional resilience factors to maintain better psychosocial outcomes. However, having more resilience factors may not help offset the physical health costs of disadvantaged neighborhoods.
(019) SEXUAL HISTORY-TAKING BY TUNISIAN PRIMARY HEALTHCARE PROVIDERS AND PATIENT-RELATED INFLUENCING FACTORS
Authors: Emna M , Kaabia O , Mohamed Hichem S , Ben Hassine SA , Cherif W , Cheour M
Published: December 2024

Introduction

Sexual history-taking is a crucial aspect of comprehensive healthcare. Understanding the dynamic of this interaction between primary health care providers and patients is important for improving the quality and depth of sexual history discussions.

Objective

We aim to explore the landscape of sexual history-taking practices among Tunisian family physicians and the various patient-related factors influencing them. By identifying barriers and facilitators, we can promote better patient-provider communication, ultimately enhancing the delivery of sexual healthcare services.

Methods

We conducted this cross-sectional study among Tunisian family medicine physicians and residents, using an anonymous online self-administered questionnaire in French.

Results

The study involved 400 participants, predominantly female (72.1%). It included general practitioners (40.8%), family medicine residents (38.2%), and physicians (21%). In our survey, 81.3% of participants indicated infrequent collection of patients’ sexual histories. Such inquiries occurred primarily when the chief complaint was urogenital (80%). According to participants, their approach was significantly influenced by patient’s gender (25.4%) and age, especially for patients under 18 and over 65, 59.7% and 45.4%, respectively, perceived a substantial impact on sexual history-taking. The educational level of patients significantly influenced this discussion according to 63.1%. The marital status was a considerable factor when taking sexual history according to 28% of participants. Patients’ religious contexts greatly influenced 29% of physicians. Sexual orientation significantly impacted discussions for 17.7% of participants, while 70% believed it had little or no impact. With People living with HIV, Men who have Sex with Men, Transgender individuals, Sex Workers, Injectable Drug Users, more than 20% of respondents declared experiencing “a lot” or “a tremendous amount” of difficulty addressing sexuality. Regarding medical conditions, chronic neuropsychiatric conditions were identified as a significant barrier by 42.6% of the respondents, surpassing motor or sensory impairments (22.3%).

Conclusions

Results shed light on the complexities surrounding sexual history-taking among Tunisian primary physicians. Participants revealed that their approach is significantly influenced by patients’ demographics and medical conditions. Challenges arise when addressing sexual health with key populations. These findings support the need for interventions to enhance healthcare professionals’ skills and comfort levels in addressing sexual health, ensuring a more comprehensive and inclusive care.

Disclosure

No.
(027) THERAPEUTIC EFFICACY OF HUMAN AMNIOTIC FLUID-DERIVED STEM CELLS IN RESTORING ERECTILE FUNCTION FOLLOWING CAVERNOUS NERVE-SPARING PROSTATECTOMY: EVIDENCE FROM A PROSTATE REMOVING-ERECTILE DYSFUNCTION RAT MODEL
Authors: Tsai W , Wu Y , Hsu W , Chiang H
Published: December 2024

Introduction

Erectile dysfunction (ED) after Nerve-sparing radical prostatectomy (NSRP) is a major concern for prostate cancer patient at far. The therapeutic potential of human amniotic fluid-derived stem cells (hAFSCs) in the treatment of ED is promising, but not fully understood.

Objective

Since cavernous nerve-sparing prostatectomy (CNSP)-ED is best animal model which mimic the ED caused by NSRP, In the CNSP injury rat paradigm, we aimed to determine the effect of application of hAFSCs after prostate removed without nerve injury and their effects on erectile function (EF) preservation.

Methods

We divided 24 number of 8-week-old Sprague–Dawley rats into three groups: sham (Group 1), saline intracavernosal (IC) injection after CNSP (Group 2), and hAFSC IC injection after CNSP (Group 3). hAFSCs from the fourth passage had the potential to differentiate into trilineage cells. All animals underwent EF analysis on the 28th day post-injection. To assess EF, we measured the mean arterial pressure (MAP) and intracavernosal pressure (ICP) in response to cavernous nerve (CN) stimulation. We also harvested the penis and major pelvic ganglion (MPG) for immunohistochemical and electron microscopy analyses.

Results

The rats treated with hAFSCs showed significantly higher ICP responses (the ratio of maximum ICP to MAP was 0.57 ± 0.04) than the CNSP rats (the ratio of maximum ICP to MAP was 0.38 ± 0.05). Immunohistochemical analysis revealed increased expression of α-SMA and vWf in the corpus cavernosum and enhanced expression of nNOS in the dorsal penile nerve (DPN) (p < 0.05) and MPG of CNSP-ED rats. Electron microscopy revealed unmyelinated axonal reconstruction of CN and maintained corporal smooth muscle structures in CNSP-ED rats treated with hAFSCs.

Conclusions

hAFSCs treatment preserved EF in CNSP-ED rats at a single dose. The results of this study show the potential of hAFSCs as a novel application in the treatment of ED after prostatectomy.

Disclosure

No.
(028) SAFETY AND EFFICACY ASSESSMENT OF THE VERTICA® - A RADIO FREQUENCY DEVICE FOR THE TREATMENT OF ERECTILE DYSFUNCTION (ED)
Authors: Abou Chawareb E , Lumbiganon S , Hammad M , Miller J , Raheem O , Reisman Y , Gruenwald I , Lischinsky D , Yafi F
Published: December 2024

Introduction

The VERTICA® is a hand-held, home use device, designed to treat “mild to moderate” and moderate erectile dysfunction (ED) using radiofrequency (RF) electrodes. These electrodes deliver sequential RF energy to the penile area, with additional electrodes on a pad applied to the perineal area to also cover the penile crus. We hypothesize that 1) RF energy raises penile temperature, enhancing erectile response, and 2) RF energy posits a direct structural and biochemical impact on tunical and corporal tissue, improving the tunical veno-occlusive mechanism.

Objective

The purpose of the current study is to evaluate the safety and efficacy of the VERTICA® investigational device for treatment of ED.

Methods

This study is a pivotal, prospective, randomized, controlled, double-blind clinical trial. Around 100, in our center 33 adult, heterosexual, and sexually active males (≥22 years) with mild to moderate and mild organic ED (IIEF EF score 11-21) will be enrolled and randomized 1:1 for Active or Sham VERTICA® treatment. Patients are instructed to use the device 3 times a week for 4 weeks (12 treatments), twice a week for an additional 4 weeks (8 treatments) and then 1-2 times a week for another 4 months. Every treatment comprises a 15-minute session involving ring electrodes, followed by an additional 15-minute session incorporating both ring and pad electrodes. Compliance and treatment data is monitored using a smartphone app. Subjects are instructed to attempt sexual activity weekly or 6 times a month, logging events in the Sexual Encounter Profile (SEP) form. Monthly follow-up assessments at months 1, 2, 3, and 6 include evaluating the efficacy of the treatment using the IIEF-EF, SEP, EHS, EDITS, and SHIM questionnaires. Enhancements in ED will be measured by comparing the scores of these questionnaires within both groups and, for every individual, before and after completing the study.

Results

A pilot study with 28 subjects demonstrated the device’s safety and efficacy, with no serious adverse events reported in 144 treatment sessions.

Conclusions

In conclusion, the VERTICA® investigational device shows promise in the treatment of mild to moderate erectile dysfunction (ED) based on the pilot study and the ongoing pivotal clinical trial. If the results of this trial are promising, the VERTICA® device could offer a novel and effective treatment option for men with mild to moderate ED, potentially improving their quality of life and sexual health.

Disclosure

Yes, this is sponsored by industry/sponsor: OhhMed.

Clarification: Industry funding only - investigator initiated and executed study.

Any of the authors act as a consultant, employee or shareholder of an industry for: Sprout.
(047) SEXUAL HEALTH AMONG WOMEN WITH THYROID DISORDERS
Authors: Kaabia O , Gharbi I , Smaali H , Guefrech O , Maya Bahri Y
Published: December 2024

Introduction

Thyroid disorders are one of the most common endocrine disorders worldwide. Thyroid disorders are known to impact the patients’ quality of life and their sexual health and function, especially among women.

Objective

This study aims to assess any association between sexual dysfunction and thyroid disorders.

Methods

This is a nested case–control study where the cases were women self-reporting thyroid disorder and controls were self-reported healthy women matching for medical history, age, and relationship status. Data was collected through an online self-administered questionnaire. The sexual function was assessed through validated translations of the Female Sexual Function Index. Female sexual dysfunction is defined by an FSFI score ≤ 26.55.

Results

This study included 15 women with thyroid disorders and 30 controls. Age and other main socio-demographic components were similar between the 2 groups. The prevalence of female sexual dysfunction among thyroid disorder patients was 33.33% compared to 56.66% in the controls. (p = 0.20). There was no statistical difference between both groups in terms of total FSFI score (p = 0.34). There was also no difference between the groups among the six FSFI domains’ scores (Desire, Orgasm, Excitation, Lubrication, Pain, and Satisfaction).

Conclusions

This case–control study shows no difference in terms of sexual function between women self-reporting thyroid disorders and healthy young women. Although the literature may claim that thyroid disorders might be associated with altered sexual health among women, our population was under treatment at the time of the study, and thyroid hormonal treatment may have interacted with patients’ sexuality.

Disclosure

No.
(054) CAN WE IMPROVE TIME TO PATENCY WITH VASOEPIDIDYMOSTOMY WITH AN INNOVATIVE EPIDIDYMAL OCCLUSION STITCH?
Authors: Costantini Mesquita F , Savio LF , Velasquez DA , Aponte Varnum A , Barros R , Miller D , Petrella F , Ramasamy R
Published: December 2024

Introduction

Obstructive azoospermia, affecting 40% of azoospermia cases, occurs when a blockage in the male reproductive tract leads to no sperm in the ejaculate. Blockages may be congenital or acquired, affecting the epididymis, vas deferens, or ejaculatory ducts. Congenital causes include conditions like congenital bilateral absence of the vas deferens, while acquired causes involve vasectomy, infections, trauma, or medical procedures. Treatment includes microsurgical reconstruction or sperm extraction for IVF, with microsurgery being cost-effective. Success rates are 99% for vasovasostomy (VV) but drop to 65% for vasoepididymostomy (VE). Refinement of VE techniques is crucial for improving outcomes.

Objective

A 39-year-old male with obstructive azoospermia underwent VE. His partner, 37, has regular menstrual cycles. Clinical data included FSH levels, semen analysis, and testicular sperm aspiration results.

Methods

The procedure began with the right vas deferens. A vasogram showed no obstruction. No fluid from the testicular side led to VE. Dilated epididymal tubules were identified, and motile sperm was found. A novel epididymal occlusion stitch was applied to enhance sperm passage through the anastomosis. The steps were repeated on the left. At a 3-month follow-up, the patient had motile sperm in his semen analysis.

Results

The epididymal occlusion stitch aims to improve VE success. The 3-month follow-up showed promising results with motile sperm presence. Historical success rates for VE have remained low with delayed patency and higher failure rates. Enhancements in VE techniques are needed to improve outcomes.

Conclusions

This video highlights a novel technique to improve VE success rates. The epididymal occlusion stitch shows promise in reducing patency time and enhancing outcomes, marking progress in male reproductive surgery.

Disclosure

No.
(062) BIPOLAR ELECTROSURGICAL SCISSORS IN OPEN ANDRO-SURGERY. THE HUNT FOR HEMOSTASIS AND HASTE
Authors: Mahmoud O
Published: December 2024

Introduction

Controlling hemostasis is a main part of any surgery, and can be time consuming in some. Electro-surgical cautery is a fundamental way to control bleeding. Bipolar electro-cautery limits tissue devitalization by limiting energy spread. Bipolar Scissors (BPS) combine scissor-cutting process and bipolar diathermy in one instrument and one surgical step. Preserving tissue viability and a short operative time improve surgical outcome.

Objective

To study the outcome of using BPS in inflatable penile prosthesis (IPP), Shaeer’s corporal rotation for correction of severe ventral penile curvature (SCR) and suprapubic dermo-lipectomy (DL) for revealing the concealed penis.

Methods

The BPS group was compared to a control group where BPS was not utilized. BPS were used to cut through Dartos and Buck’s fascia, mobilize the neurovascular bundle and excise suprapubic fat. Operative time and drain output were evaluated.

Results

Mean operative time was significantly shorter in BPS group by 13.6% for IPP surgery, 18.7% for SCR and 23.6% for DL. Mean drain output was significantly less in BPS group by 50.5% in IPP and 35.4% in DL. Peno-scrotal edema was less pronounced and resolved faster when BPS was used.

Conclusions

Bipolar scissors offer better hemostasis and a shorter operative time in penile prosthesis, penile curvature and pubic lipectomy surgeries.

Disclosure

No.
(064) A NOVEL SURGICAL TECHNIQUE TO FACILITATE FEMALE ORGASM
Authors: Unal C , Aydin S
Published: December 2024

Introduction

Female orgasmic disorder is defined as marked infrequency or absence of orgasm and the distress caused by the situation. The approach to treatment is based on psychosocial treatment models. Directed masturbation, sensate focus exercises, exposure and cognitive behavioral therapy are the psychosocial treatment options defined. Surgical reconstruction and adjustment or cosmetic surgery serve as a different yet limited way for treatment. The evidence shows that the clitoral anatomic topography may affect female sexual function; longer the clitoro-urethral distance was found to be correlated with lower Female Sexual Function Index (FSFI) Orgasm sub scores.

Objective

We aim to introduce a surgical technique, to be performed to women whose clitoro-urethral distance is more than 25 millimeters and having difficulty in reaching orgasm.

Methods

The medial faces of the labia minora are planned to be partially excised by drawing rhomboid figures on both sides. Horizontal wound closure was performed via separated suturing technique with 3.0 absorbable monofilament suture. After the clitoro-uretral distance reduction, a crescent incision was performed superficial to clitoris. Separated suturing technique with 3.0 absorbable monofilament suture was preferred for wound closure. Perineal tear due to vaginal delivery was reconstructed by routine colporrhaphy posterior.

Results

Patient with the complaint of having difficulty in reaching orgasm was evaluated, she had no medical history apart from a vaginal delivery in 2019. She has been sexually active for 8 years and 4 years ago she had cognitive behavioral therapy. The findings were rectocele grade 3 and the distance between the clitoris and urethra was found to be 25 millimeters. Preoperative FSFI score was found to be 17.6, with a remarkable orgasm sub score showing 2. Colporrhaphy posterior, clitoro-urethral distance reduction and clitoral hood reduction was performed. Postoperative healing period was uneventful. Postoperative sixth month FSFI score was 25.8 with an orgasm sub score 4.2.

Conclusions

Surgical approach the female orgasmic disorder is not the first line approach, however in a chosen group of patients with a clitoral urethral distance longer 25 millimeters, the reduction procedure may serve as an option.

Disclosure

No.
(085) THE PATIENT-PERCEIVED INTRAVAGINAL EJACULATION LATENCY TIME CUT-OFF FOR LIFELONG PREMATURE EJACULATION
Authors: Wang C , Zhong Q , Colonnello E , Xi Y , Sansone A , Zhang H , Chen Q , Jannini EA , Zhang Y
Published: December 2024

Introduction

The current cutoff values of IELT for diagnosing lifelong premature ejaculation (LPE) do not always match clinical practice, and the inconsistency in the cutoff values of IELT among different definitions has also posed challenges to both clinical management and research of PE.

Objective

To re-evaluate the IELT and to find evidence-based cut-off values for diagnosing LPE that can be widely accepted and match clinical practice.

Methods

We addressed the flaws of previous studies. LPE was diagnosed based on both patient self-report of PE status and scores on the Premature Ejaculation Diagnostic Tool (PEDT), with a control group consisting of non-PE individuals included for comparison. Utilizing receiver operating characteristic (ROC) curve analysis, the optimal self-estimated cutoff value for IELT in diagnosing LPE was determined.

Results

A total of 307 heterosexual participants (mean age = 30.7 ± 6.4) were included, comprising 187 LPE patients (mean age = 28.0 ± 4.6) and 120 non-PE individuals (mean age = 35.0 ± 6.5). 2.7% of LPE patients experienced anteportal ejaculation. 59.9%, 92%, and 97.9% of LPE patients displayed IELTs within 1, 2, and 3 minutes, respectively. The ROC curve’s AUC was 0.996 with a 95% CI of 0.991–1.000 (p < 0.0001). The P-IELT cut-off at 3.5 (sensitivity = 97.9%, specificity = 99.2%) showed the highest Youden index compared with other options.

Conclusions

The P-IELT cutoff value for diagnosing LPE within a clinical practice context is 3.5 minutes.

Disclosure

Any of the authors act as a consultant, employee or shareholder of an industry for: EAJ is or has been paid consultant and/or paid speaker for Bayer, Ibsa, Menarini, Otsuka, Pfizer, Viatris.
(098) DOES METABOLIC SYNDROME HEIGHTEN THE RISK OF SEXUAL DYSFUNCTION IN BOTH MEN AND WOMEN?
Authors: Dilixiati D , Lu J , Kadier K , Chen F , Qiao B , Ge C , Zebibula A , Rexiati M
Published: December 2024

Introduction

Previous research has examined the link between Metabolic Syndrome (MetS) and male sexual dysfunction. However, there is limited and conflicting evidence regarding MetS and Female Sexual Dysfunction (FSD).

Objective

We investigated the correlation between MetS and both ED and FSD through two-sample Mendelian randomization(MR) and meta-analysis methods, respectively.

Methods

A two-sample MR study was conducted to investigate the causal relationship between MetS and its five individual components (hypertension, waist circumference (WC), fasting blood glucose (FBG), triglycerides, and high-density lipoprotein cholesterol (HDL-C)) and ED. In addition, a systematic search of four databases (the PubMed, Scopus, Embase and Cochrane Library databases) was conducted from inception to 23 August, 2023.

Results

In MR study, our observation revealed that hypertension (OR = 1.104, 95% CI: 1.014 − 1.202, p = 0.023) and WC (OR: 1.279, 95% CI = 1.101-1.486, P = 0.001) exhibited an increased genetic predisposition to ED. However, no causal effects of MetS, FBG, triglycerides and HDL-C on ED were observed in our study. The results of the meta-analysis showed a significant 1.746-fold increased risk of FSD in individuals with MetS compared to the control group (OR = 1.746, 95% CI = 1.359–2.241, P < 0.001).

Conclusions

Our study provides genetic evidence supporting the causal relationship between hypertension, WC and ED, rather than MetS itself, FBG, triglycerides, or HDL-C. In addition, our systematic review and meta-analysis results suggest that patients with MetS have a significantly increased risk of FSD.

Disclosure

No.
(111) SAFETY OF AN ELASTOMER-BASED HOOK-AND-LOOP CONFIGURATION “SMART” PENILE CONSTRICTION DEVICE WORN OVERNIGHT AKA: IS IT OK TO FALL ASLEEP WITH THIS COCKRING?
Authors: Winter A , Gross K , Dubin J , Justin E
Published: December 2024

Introduction

Penile constriction devices are commonly used for the management of erectile dysfunction, climacturia, and general sexual performance enhancement, as a stand-alone or multimodal therapy. One common concern is that prolonged utilization of a penile constriction device can lead to difficulty with device removal, penile injury, and potentially penile necrosis.

Objective

With traditional penile constriction devices, there is no way to objectively monitor safety or aggregate user experience data. We hypothesize that a novel elastomer based hook-and-loop configuration cock ring is safe for use overnight, and that biometric sensors embedded in the device can document spontaneous nocturnal erections- thereby confirming presence of blood flow to the penis hours into wear.

Methods

A novel elastomer-based hook-and-loop configuration penile constriction device with embedded pressure sensors was worn by participants in a home environment. Recordings of erection rigidity and duration were uploaded to participants’ smart-phone app via Bluetooth, and data was centrally aggregated by the app engineering team. Subjective reports of user experience were aggregated via narrative input.

Results

5204 unique recordings were obtained over a 14 month period. Mean duration of recording was 6.57+/-3.92 hours. 2700 recordings were greater than 4 hours in duration, with an average 3.09+/-1.62 erections per recording. There were no reports of priapism or dermatitis reported across all users. Two users noted discomfort after >4 hours of use, but they were able to release the device without difficulty.

Conclusions

A biometric sensor enabled “smart” cockring, composed of elastomer in a novel hook-and-loop configuration was worn >5000 individual times with an average duration of >6 hours of wear. There were no significant adverse events, and multiple erections were documented for users who kept the device on for >4 hours, indicating physiologic blood flow to the penis. To our knowledge, this is the first penile constriction device to have proven safety with overnight use.

Disclosure

Yes, this is sponsored by industry/sponsor: FirmTech.

Clarification: Industry initiated, executed and funded study.

Any of the authors act as a consultant, employee or shareholder of an industry for: FirmTech.
(125) BASAL RELEASE OF 6-CYANODOPAMINE FROM RAT ISOLATED VAS DEFERENS AND ITS ROLE ON THE VAS DEFERENS CONTRACTILITY
Authors: Sanches Dal Pozzo CF , De Nucci G , Britto-Júnior J , Agostini Badin JF , Fragonesi A , Lorenzon F
Published: December 2024

Introduction

6-Cyanodopamine is released from isolated rabbit heart and is a novel endothelial catecholamine similar to 6-nitrodopamine described by our research group. 6-Nitrodopamine is a major endogenous modulator of human vas deferens contractility. However, it is not known whether 6-cyanodopamine presents any biological activity.

Objective

Here it was evaluated whether 6-cyanodopamine was released from rat isolated vas deferens and wether, like 6-nitrodopamine, modulates vas deferens contractility.

Methods

The basal release of 6-bromodopamine, 6-nitrodopa, 6-nitrodopamine, 6-cyanodopamine, and 6-nitroadrenaline from the vas deferens was quantified using LC–MS/MS. Electric-field stimulation (EFS) and concentration-response curves for noradrenaline, adrenaline, and dopamine were performed on the rat isolated epididymal vas deferens (RIEVD) in the absence and presence of 6-cyanodopamine. The expression of tyrosine hydroxylase and S100 was assessed by immunohistochemistry. Additionally, we evaluated the effect of tetrodotoxin on the release and effect of 6-cyanodopamine in the rat vas deferens. The synergism between 6-cyanodopamine and 6-nitrodopamine was analyzed in the RIEVD.

Results

The rat isolated vas deferens release both 6-cyanodopamine and 6-nitrodopamine. Pre-incubation with tetrodotoxin, had no effect on the release of 6-cyanodopamine, but it virtually abolished 6-nitrodopamine release. 6-Cyanodopamine contracted RIEVD only at 1 mM, but significantly potentiated the contractions induced by both noradrenaline and EFS at 1 nM. At 10 and 100 nM, 6-cyanodopamine also significantly potentiated the RIEVD contractions induced by adrenaline and dopamine. The potentiation of both noradrenaline and adrenaline contractions by 6-cyanodopamine was present in tetrodotoxin-pretreated tissues. Co-incubation of 6-cyanodopamine (100 pM) with 6-nitrodopamine (10 pM), caused significant leftward shifts and increased maximal responses of the concentration-response curves to noradrenaline, even in the presence of tetrodotoxin. Immunohistochemistry on RIEVD revealed the presence of tyrosine hydroxylase in both the cytoplasm of epithelial cells of the mucosae and in the nerve fibers.

Conclusions

The identification of the basal release of epithelium-derived 6-cyanodopaine from rat isolated vas deferens and its remarkable ability to potentiate noradrenaline- and EFS induced contractions of this tissue, demonstrate that epithelial cells modulate smooth muscle contractility.

Disclosure

No.
(129) TECHNICAL ASPECTS OF THE PENILE IMPLANT IN THE TRANSGENDER MALE PATIENT
Authors: Hendges LP , Martinez-Fernandez A , Vishwakarma R , Maiolino G , Fernández-Pascual E , Lledó-Garcia E , Martinez-Salamanca JI
Published: December 2024

Introduction

The surgery for the penile prosthesis in transgender male patients with neophallus is technicaly challenging. The absence of native penile structures and the presence of a single vascular pedicle for the neophallus vascularization, usually are factors that stand against the prosthetic surgeon. Thefore, the complication rate of this procedures generally are higher than the cisgender patient.

Objective

The ain of this abstract is to elucidate and standardize the surgical steps of this procedure using are experience with the new prosthesis models specifically designed for the transgender male patient.

Methods

We overview, the surgical steps, and nuances, of the penile prosthesis in the transgender male patient. We show 2 surgical cases using the inflatable prosthesis (ZSI 475 FTM) and the malleable prosthesis (ZSI 100 FTM) both designed for the neophallus. We surgically standardized the technique utilizing an infrapubic aproach, on the contralateral side of the vascular pedicle. We performe the neophallus dilatation leaving 1 cm of fat pad above the dilation tunel, and 2 cm above de glans skin, to possbility a natural aspect and avoid a superficial location of the implant. The proximal aspect of the prosthesis are fixated on the pubic bone. In the inflatable prosthesis, the pump is placed in the neoscrotum and simulate a testicle prosthesis. The resorvoir is placed in the Retzius Space as in the infrapubic aproach in the cismale patients.

Results

The patients did not report any complications and are fully satisfied with the implant.

Conclusions

The surgical aspects of the technique for the penile prosthesis in the transgender male patient, despite defying, if standardezed could be the way to decrease the complication rate in this tipe of surgery. The newest prosthesis models assists to improve the surgicals outcomes, and patient satisfaction.

Disclosure

No.
(133) ERECTILE DYSFUNCTION IN MEN UNDERGOING HEMODIALYSIS FOR CHRONIC KIDNEY DISEASE IN A CITY IN THE BRAZILIAN AMAZON
Authors: De Barros Neto J , Afonso Teixeira T , Rocha Lopes G , Santos Almeida HK , Prado Dantas HV , Garcia De Azevedo ME , Tenório Da Silva F , Dias Rocha K , Tiyomi Sacata Tongu Nazima M
Published: December 2024

Introduction

Erectile dysfunction (ED) is a prevalent sexual issue that affects men with chronic kidney disease (CKD). The degree of sexual dysfunction generally worsens with the progression of CKD.

Objective

we assessed the erectile function and sexual quality of life of adult male CKD patients who undergo hemodialysis (HD) in a hospital situated in the Brazilian Amazon.

Methods

A cross-sectional quantitative study was conducted in the HD Sector of the Nephrology Unit. The study included men with CKD who were 18 years of age or older and had been sexually active for at least six months. These men underwent three weekly HD sessions for three months. To measure sexual satisfaction, we used the Male Sexual Quotient (MSQ). We also used the International Index of Erectile Function (IIEF5) to assess erectile function. We performed statistical analysis with SPSS 21.0, utilizing appropriate tests such as Mann–Whitney and Kruskal-Wallis (P > 0.05).

Results

Ninety-eight patients with an average age of 51.68 ± 15.28 years were evaluated. Most were married or in a relationship (60.20%), had been receiving HD treatment for 1 to 5 years (55.10%), and had an average KTV of 1.17. The prevalence of ED was found to be 66.30%. It was observed that ED was more common in older patients (p = 0.01), those with lower family income (p = 0.02), diabetes (p = 0.01), lower mean corpuscular hemoglobin (p = 0.04), higher total calcium (p = 0.04), and lower albumin (p = 0.03). Around 75% of patients rated their sexual life as regular to excellent.

Conclusions

Despite a high prevalence of ED, men with CKD undergoing HD reported regular to excellent sexual function. The study emphasizes the importance of screening and routine evaluations for sexual issues in these patients.

Disclosure

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(187) OBSTRUCTED HEMIVAGINA AND IPSILATERAL RENAL ANOMALY (OHVIRA) SYNDROME: CASE SERIES
Authors: Monteiro Filho M , Nacle L , Ciriaco M , Monteiro P , Bruno Z
Published: December 2024

Introduction

Obstructed Hemivagina with Ipsilateral Renal Anomaly (OHVIRA) syndrome, also known as Herlyn-Werner-Wunderlich syndrome, is a rare anomaly of the female genitourinary tract characterized by the triad of uterus didelphys, an obstructed vagina due to an oblique vaginal septum, and ipsilateral renal agenesis. Affected women may present with cyclic pelvic pain, dysmenorrhea, and/or hematocolpos a few years after menarche.

Objective

To describe the similarities and particularities of patients with OHVIRA syndrome followed in a specialized outpatient clinic of a tertiary university hospital.

Methods

Retrospective, cross-sectional, and quantitative study based on the review of medical records from November 2016 to April 2024. The collected data were organized in a spreadsheet and presented as mean, number, and percentages.

Results

Fourteen patients with OHVIRA syndrome were identified, who had menarche at an average age of 10.8 years, except for one patient who had not yet had menarche at the time of data collection. After the first menstruation, an average of 11 months elapsed before the onset of pelvic pain. Consistent with the natural history of the syndrome, all patients were diagnosed during childhood or adolescence, with an average age of 12.7 years, the youngest being diagnosed at seven and the oldest at 16. Of the 14 patients followed, 10 underwent vaginal septoplasty an average of 14.4 months after menarche. Of these, only one required a second septoplasty to ensure vaginal patency, six months after the first procedure. Only one patient, who had a four-year interval between menarche and diagnosis, developed endometriosis.

Conclusions

Progressive dysmenorrhea complaints at the beginning of reproductive life should not be minimized and warrant appropriate investigation. The diagnosis of OHVIRA syndrome is low-cost and easily accessible, as it is made through clinical examination and ultrasonography. Besides diagnosis, timely intervention is essential to avoid sequelae resulting from vaginal obstruction, such as endometriosis and chronic pelvic pain. Therefore, the identification of the clinical triad and early approaches can significantly impact the quality of life of patients with this condition.

Disclosure

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(193) EPIDEMIOLOGICAL CHARACTERISTICS OF ERECTILE DYSFUNCTION IN THE BRAZILIAN POPULATION: A CROSS-SECTIONAL STUDY
Authors: Noffs Motta S , Yoneyama Mourthé E , Rocha Chavez Zambrana D , Victor Fonseca Ribeiro J , Nardozza Junior A , Rhein Felippe M , Iasmin Lima Lemos C , Eli Girotti M , Brandão Vasco M
Published: December 2024

Introduction

Erectile dysfunction (ED) is a common symptom among men with cardiovascular diseases and various disorders, including hypogonadism, Peyronie’s disease, priapism, neuralgic disorders, and depression, as reported by the American Institute of Health. The presence of these conditions serves as risk factors for the development of ED, prompting men to seek medical attention when they detect changes in erectile function, thereby signaling potential underlying health issues. Understanding the link between these conditions and ED is crucial for effective management and intervention.

Objective

This study aims to investigate the epidemiological profile of men diagnosed with erectile dysfunction.

Methods

A retrospective cross-sectional analysis was conducted on patients with erectile dysfunction attending the andrology outpatient clinic at a university hospital. Sociodemographic information, comorbidity profiles, lifestyle habits, addictive behaviors, and other sexual concerns were assessed through a structured questionnaire. The association between sociodemographic variables and comorbidities was evaluated using Fisher’s exact test, with statistical significance set at p < 0.05, and analyzed using the Stata® software.

Results

A total of 654 patients were included in the study, with a mean age of 61.46 ± 12.01 years, and 63.5% of participants aged ≥60 years. The racial distribution showed that 54.6% were white (357/654) and 32.2% were brown (211/654) individuals. Among them, 68.3% (447/654) were married, and 50.9% (333/654) had not completed elementary education. The most prevalent comorbidities were Systemic Arterial Hypertension (51%, 333/654) and Diabetes Mellitus (32.7%, 214/654). Regarding lifestyle habits, 77.1% (504/654) did not use any drugs, while 16.7% (109/654) were smokers. Additionally, 80 patients reported concurrent sexual dysfunctions, with premature ejaculation (33%, 26/80) and Peyronie’s disease (25%, 20/80) being the most common. Smoking was found to have a significant association with ED (p-value = 0.03), whereas no other statistically significant associations were observed in the analyzed variables.

Conclusions

This study highlights that individuals with ED in this population tend to be predominantly white or brown, married, and with lower educational attainment. Systemic Arterial Hypertension and Diabetes Mellitus were the leading comorbidities, and smoking showed a significant association with ED. These findings underscore the importance of comprehensive assessment and management strategies for ED patients, taking into account their sociodemographic and lifestyle factors.

Disclosure

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(219) SUCCESSFUL HANDLING OF VAGINISM BY HYPNOSIS
Authors: Csiby A
Published: December 2024

Introduction

The clinical demonstration describes the treatment of vaginism - vaginism occuring before and during a gynecological examination - with hypnosis. The 33-year-old female patient had been suffering from continuous lower abdominal pain for 3 years. Endocrinological and laboratory tests showed negative results. A gynecological and a vaginal ultrasound examination were necessary to rule out or diagnose endometriosis, however the vaginism that occurred during the examinations and the intense anxiety that appeared in the doctor’s consulting room, did not allow the examination. After 9 unsuccessful gynecological examinations, the patient turned to a psychologist on the advice of a psychiatrist.

Objective

The objective was to cure the vaginism of the patient who has been suffering by it for 3 years and visited 9 gynachologist to have the examination.

Methods

After uncovering the background of the anxiety, we used systematic desensitization in hypnosis.

Results

Psychological consultations and sessions of hypnosis made it possible to perform a vaginal ultrasound examination and establish the source of the somatic symptom.

Conclusions

The case points to the possibility that a somatic complaint related to sexuality that has existed for years can be successfully treated with hypnosis.

Disclosure

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(227) PRELIMINARY DATA ON THE USE OF PLATELET RICH PLASMA ON PENIS FOR ERECTILE DYSFUNCTION
Authors: Mayer M , Valente S , Gioielli A
Published: December 2024

Introduction

Erectile dysfunction (ED) is the persistent or recurrent inability to achieve and/or maintain a penile erection sufficient to maintain satisfactory sexual encounters. For correct function, the erectile mechanism requires the integrity of the participating neurological, psychological, vascular, mechanical and hormonal components. Any impairment of any of these components can make it difficult to develop and maintain an erection. Vasculogenic ED is the main cause of organic ED and it is a disorder that is manifested by reduced penile blood flow, arterial insufficiency or stenosis, as well as endothelial dysfunction. The use of platelet-rich plasma (PRP) in multiple medical specialties is already known internationally. Its mechanism of action is at the cellular level, increasing its number (mitogenesis) and stimulating angiogenesis through multiple growth factors and cytokines that can affect inflammation, angiogenesis and proliferation. Different studies show that PRP injections can modify key pathophysiological mechanisms that lead to erectile dysfunction through reparative, anti-inflammatory, neuroprotective and neurotrophic effects. There is evidence that PRP treatment could induce a functional effect in the cavernous tissue in addition to the declared restorative/regenerative effect.

Objective

To evaluate the effects of acute exposure to autologous PRP as a therapeutic strategy in patients with erectile dysfunction of mild–moderate.

Methods

Patients with erectile dysfunction (ED) of mild–moderate calculated by IIEF 5 questionnaire, with incomplete response to IPD5 inhibitors, were selected and 4 doses of autologous PRP were applied to the penis. All the patients signed an informed consent prior to each application.

Results

The average age of the patients was 67.2 years (range 55-81). The IIEF-5 average prior to applications was 15.6. One month after having finished the complete protocol, the IIEF-5 score showed a result of 19.9. All the patients who received PRP were more satisfied with the PRP treatment with the achieved erection. No adverse effects were observed during the study period.

Conclusions

Our results support the clinical improvement of PRP treatment to IPDE5 response. Furthermore, as it is an autologous treatment, without relevant complications and at a low cost, it could be a non-dismissable strategy for patients with ED of mild–moderate vascular origin.

Disclosure

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(231) THE SENSATE FOCUS TECHNIQUE EVALUATION IN TREATING VAGINISMUS
Authors: Fejza H , Icka E , Kraja-Bardhi E , Alidema F
Published: December 2024

Introduction

Vaginismus is poorly understood and relatively unknown among healthcare providers. It can cause distress, relationship problems, and also infertility. Many treatments have been tried including sex therapy, education, hypnosis, and drug treatments.

Objective

The study aimed to present the effects of the sensate focus technique in treating vaginismus and the success rate in our clinic.

Methods

The treatment included a weekly session, each of 45 minutes duration, based on the components of the sensate focus technique to improve communication and fortify relationships, along with step-by-step learning of sexual relations, counseling and education about the anatomical and psychological basis of vaginismus for both partners. The study includes 62 cases of vaginismus treated in the Specialized Clinic of Clinical Sexology- Libido in Pristina, Kosovo, during the period of, October 2019- March 2024.

Results

Sensate focus techniques usually include three sessions, but in our cases, we treated some patients longer. In 29% of cases, the problem was solved by 1-3 sessions, while 43% were in 3-5 sessions. Cases mainly with degrees 4 and 5 of vaginismus were treated in more than 5 sessions.

Conclusions

The sensate focus technique is the most appropriate method for solving problems with vaginismus. Taking into consideration the predisposing and maintenance factors, the information and advice given by this method make changes in beliefs and behaviors during sexual activity.

Disclosure

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(237) TRANSLATION, SEMANTIC EQUIVALENCE, AND CULTURAL ADAPTATION OF THE SEXUAL INTERVENTION SELF-EFFICACY
Authors: Finotelli Junior I
Published: December 2024

Introduction

Sexuality matters and is a mental health indicator. Awareness of sexual studies has expanded, overcoming misconceptions and underestimating their social impact. Global research shows that the issue goes beyond bias due to poor education and post-college training. Few English-language and culturally limited measures assess professionals competency on these issues.

Objective

This study aims to translate and culturally adapt the Sexual Intervention Self-Efficacy Scale to Brazilian Portuguese. It’s an initial step toward a national scale aligned with Brazilian academic and legal standards.

Methods

This 19-item scale was designed to assess self-efficacy beliefs regarding the ability to address clients’ sexual concerns/problems in a clinical setting. The items are measured on a Likert scale, with responses ranging from “strongly disagree” to “strongly agree”. The process engaged twelve sexuality specialists with over 15 years of experience, and a convenience sample of 60 individuals assessed intelligibility. The stages included translation, back-translation, evaluation of semantic-equivalence, final review by experts, and pre-test version.

Results

Assessing semantic equivalence demonstrated substantial agreement among judges, ranging from strong to perfect. The expert review updated the technical terminology, as the scale was created over 10 years ago, and modified the item about concerns to address discomfort, not exclusively related to discussing masturbation. There were no significant considerations in the applications conducted in the pre-test version.

Conclusions

The findings presented a version that was extremely intelligible and placed significant emphasis on updating technical terms. It has the potential to serve as a screening tool to identify topics that need to be developed by professionals. This version kept the scales name in English but added “Brazilian Adaptation” in Portuguese at the end.

Disclosure

No.