bluuebunny/crossref_metadata_2025 huggingface dataset:
EFEK ANTIBAKTERI EKSTRAK KULIT BUAH DELIMA (PUNICA GRANATUM) TERHADAP SHIGELLA DYSENTRIAESECARA IN VITRO
Authors: Prihantoro Teguh , Indra Rasjad , Sumarno Sumarno
Published: January 2017

Shigella dysentriae is the most common organisms which causes bacillary dysentery in developing countries with highÂ mortality. Pomegranates (Punica granatum) have beenused since a long time ago in folk medicine for several purposes.Â The antibacterial activity was linked to the presence of major phytocompounds like flavonoids, triterpenes, phenols, andÂ ellagitannin. The aim of this experimental study wasto evaluate the antibacterial effect of Pomegranateâ€™s (PunicaÂ Granatum) rind extract against Shigella dysentriae Â and to determine the relationship between concentrations ofÂ Pomegranateâ€™s rind extract with the growth of Shigella dysentriae in vitro. Antimicrobial effect was determined by tubeÂ dilution method. This consists of determination of Â Minimum Inhibitory Concentrations (MICs), and Minimum BactericidalÂ Concentrations (MBCs). The concentrations of Pomegranateâ€™s (Punica Granatum) rind extract were 0.8%, 1%, 1.2%, 1.4%,Â 1.6%, and 1.8%. At low extract concentration (0.8%)bacterial growth was delayed, while at higher extract concentrationÂ (1.8%) bacterial growth was elimanated (ANOVA, p = Â 0.000, Regression R = -0.901)
EKSPRESI CYTOSOLIC ASPARTATE-SPECIFIC CYSTEINE PROTEASE-3 (CASPASE-3) PADA JARINGAN HATI RATTUS NORVEGICUS (WISTAR) SETELAH PEMBERIAN SUBKRONIK AFLATOKSIN B1 (AFB1)
Authors: Maritha Indah Dina , Supranowo Supranowo , Lyrawati Diana
Published: January 2017

Aflatoksin B1(AFB1) is one of the toxic agents produced by Aspergillus flavus that frequently contaminates foods notÂ properly stored. AFB1 undergoes biotransformation which may result in the production of reactive oxygenspecies (ROS)Â hazardous to liver cells. Following a cascade of oxidative reaction, ROS will cause the mitochondria toreleaseÂ cytochrome c which subsequently activates caspase-3, leading to apoptosis. In this present study we evaluated the effectÂ of aflatoxin B1 on the expression of caspase-3 in the liver. AFB1 was administered per oral, at different dosage andÂ length of exposure, subchronically. This study was carried out as a factorial designed experiment with Â two factors. TheÂ first was dosage factor i.e 0, 10, 15 and 20 Âµg (0; 0,05; 0,075; 0,1 Âµg/g BW) and the second was exposure time factor i.e.Â 12, 16 and 20 weeks. Rattus norvegicus strain Wistar aged approximately eight weeks old and weighed 180-200 g wereÂ used as the experimental animals. The expression of Â caspase-3 was examined by using immunohistochemistry. TheÂ results showed that the expression of caspase-3 increased significantly (p = 0,000) with the escalation of AFB1 in dosageÂ and/or exposure time (p = 0,001). In the interaction between dose and exposure time of AFB1an increase in theÂ expression of caspase-3 was also observed (p = 0,000). Interestingly, these studies also revealed thatin the liver tissuesÂ there was a limitation in the expression of caspase-3, where the raising of further AFB1 dosage and length of exposureÂ were not followed by further increase of the caspase-3.
PERBEDAAN PENGARUH PEMBERIAN MINYAK KELAPA SAWIT (Palm Oil) DAN MINYAK KELAPA MURNI (Virgin Coconut Oil) TERHADAP PERBAIKAN PROFIL LEMAK (KOLESTEROL) PADA TIKUS DENGAN DIET ATEROGENIK
Authors: Kusumastuty Inggita , Andarini Sri , Anom Aswin Anak Agung Gede
Published: January 2017

Virgin Coconut Oil (VCO) is a kind of coconut oil that most of its contained is Medium Chain Fatty Acids (MCFA) andÂ previous stdy showed that MCFA has an ability to prevent vascular desease. This experiment was done to Â prove theÂ ability of virgin coconut oil to reduce cholesteroltotal of rats with atherogenic diet compared with palm oil. This experimentÂ was designed as true pre test post test experiment with control groups. The number of samples were 24 male wistar ratsÂ and they were separated into 4 treatment groups in Â which each of them has 2 diet sections. The First section was doneÂ within 8 weeks, group A got normal diet, group B, Cand D got atherogenic diet with lard, after this section finished all ofÂ the groups got cholesterol pre test level. The next 4 weeks, in the second section treatment diet, group A got normal diet,Â group B got atherogenic diet with lard, group C gotatherogenic diet with palm oil and group D got atherogenic diet withÂ virgin coconut oil, after the second section was over rat cholesterol levels were measured by enzymatic photometric testÂ base on â€œCHO-PAPâ€. The results from One Way Anova which was continued to Tukey test showed that group B,C and DÂ were homogenous, but they were different significantly againts group A on pre test with p(0.001) < 0.05.
PENGARUH BERBAGAI DOSIS CITRININ TERHADAP KERUSAKAN STRUKTUR HEPATOSIT MENCIT (Mus musculus) PADA TIGA ZONA LOBULUS HEPAR
Authors: Hastuti Utami Sri
Published: January 2017

Citrinin is a sort of mycotoxin which caused mycotoxicosis. Citrinin is produced by Penicillium citrinum mold that canÂ contaminate sort of food especially damaged beans. Â Citrinin is known as nephrotoxic as well as hepatotoxic mycotoxinÂ and caused hepatocite structure damage. This research was done: 1) to know the effect of citrinin to hepatocite structureÂ damage; 2) to know the effect of citrinin dose difference to the value of the hepatocyte structure damage; 3) to know theÂ effect of citrinin to the value of hepatocyte structure damage on three zones of hepar lobulus. The samples of thisÂ research were male mice (Mus musculus) var. BALB-C Â at the age of three months. The experiment group of Â mice wasÂ orally administered with citrinin in three doses, i.e: dose-1: 1 mg/kg BW; dose-2: 1,75 mg/kg BW; dose-3: 2,5 mg/kg BW.Â Hepatocyte structure damage is observed on centrilobular zone, midzone, and perifer zone. The hepatocyte structureÂ damage examination is done by histopathologycal andultra structural examination by electron microscope.
PREDIKTOR ASMA PADA USIA 7 TAHUN SETELAH MENDERITA BRONKIOLITIS AKUT KARENA RESPIRATORY SYNCYTIAL VIRUS: SUATU STUDI PROSPEKTIF
Authors: Kusuma HMS. Chandra
Published: January 2017

Infants are at increased risk of developing asthma Â after RSV (Respiratory Syncytial Virus) acute bronchiolitis. TheÂ hypothesis that cytokine production is related to the development of asthma after RSV bronchiolitis. The diagnosis of RSVÂ bronchiolitis was verified using the Abbott test pack RSV, a rapid enzyme immuno assay for direct detection of viralÂ antigen in nasopharyngeal secretion. Changes in FEV1 (Forced Expiratory Volume in 1 second) were determined usingÂ spirometri, IL-4 and IFN-Î³levels were determined using the enzyme-linked immunosorbant assay (ELISA) method. In thisÂ study, 62 children hospitalized for RSV bronchiolitis were followed prospectively, 55 (88.7%) childrencompleted the studyÂ and 41 children (74.5%) had asthma at 7 years of age. RSV bronchiolitis children were more frequent among boys (boyÂ /girl ratio: 2). Episodes of wheezing more than 8 had specifity and sensitivity of 100% respectivelly,might be the veryÂ useful predictor for asthma. The cut off level over 40.84 pg/ml for IL-4, below 14.12 pg/ml for IFN-Î³, IL-4 / IFN-Î³ratio overÂ 3 and episodes of wheezing over 8 may be used to predict asthma after RCV bronchiolitis.
PENGARUH PAPARAN SINAR MATAHARI PAGI TERHADAP PENURUNAN TANDA IKTERUS PADA IKTERUS NEONATORUM FISIOLOGIS
Authors: Puspitosari Ratih Dewi , Sumarno Sumarno , Susatia Budi
Published: January 2017

Physiological jaundice is a sign usually happens tonewborn infants. The cause of icterus is the increase of indirectÂ bilirubin that makes the skin yellow that usually disturb the people. Treatment of this yellow baby is Â by exposing them toÂ the morning sun. Sun exposure can reduce jaundice skin by changing the bilirubin indirect molecule. This research aimÂ was to know the effect of morning sun exposure thatcan decrease jaundice skin through jaundice skin levelÂ measurement on babies. The special goal of the research was to know the jaundice skin and the difference before andÂ after expos to the sun. The research was a quasi experimental study. The samples consisted of 5 babies, Â selected byÂ quota sampling. The measured variables were morning sun exposure and jaundice skin level. The research was done byÂ exposing the samples on the morning sun for 30 minutes, then the jaundice skin measured in minutes 0, Â 5th, 10th, 15th,Â 20th, 25th, and 30thby using Corel Photo-Paint 12 Program. Data analysis was done with ANOVA and was continued byÂ correlation-regression test, with confidence interval 99%.
PENGARUH PEMBERIAN PbB ASETAT DOSIS TINGGI TERHADAP KETEBALAN MIELIN N. ISCHIADICUSTIKUS PUTIH (RATTUS NORVEGICUS)
Authors: Elfiah Ulfa
Published: January 2017

Lead is commonly used in batteries, electrical cabel, and pipe manufacture. Despite its usefulness, lead has someÂ sentrimental effects such as intoxication by chronic lead exposure results in peripheral neuropathy. This research was toÂ determine the effect of oral administration of leadacetate to the thickness of mielin n.ischiadicus. Â The post test onlyÂ control group design was used assigning.Thirty male Â rats into five groups of treatment. Aquadest was administered toÂ group I. Lead acetate with doses of rats were sacrificed: 65, 125, 250, and 500 mg/kgbw/day were administered to groupÂ II, III, IV, and V. Lead acetate were given daily for 10 weeks and then to make histological preparations from n.ischiadicusÂ using osmic acid as fixative and toloidin blue as staining agents.The results research showed that oraladministration ofÂ lead acetate affected the thickness of mielin with higher does resulted in thinner mielin.Â Keywords:thickness of mielin, lead acetate, oral
PENGARUH PEMBERIAN MINYAK BUAH MERAH (Pandanus conoideus) TERHADAP STRES OKSIDATIF SEL ENDOTEL YANG DIPAPAR DENGAN SERUM PENDERITA MALARIA FALCIPARUM DAN NETROFIL INDIVIDU SEHAT
Authors: Armiyanti Yunita , Fitri Loeki Enggar , Widjajanto Edi
Published: January 2017

Complication in Plasmodium falciparum malaria is associated with endothelial damage and overproductionof free radicalsÂ (oxidative stress) by activated neutrophils and endothelial cells. Red fruit (Pandanus conoideus) oil Â has a very highÂ antioxidant content, therefore it may neutralize free radicals and prevent endothelial damage. The aim of this research isÂ to prove that red fruit (Pandanus conoideus) oil reduce Reactive Oxygen Intermediate (ROI) production Â of endothelialÂ cells exposed to severe malaria patient serum and neutrophils from healthy donor. Endothelial cells from human umbilicalÂ veins were coincubated with serum from severe malaria patient and with neutrophils from healthy donor Â (positive controlÂ group). HUVECâ€™s normal was used as negative control, Â where as Â the experimental groups were given with red fruit oil inÂ different concentrations (2.8%, 5.7% dan 11.3% ). The Reactive Oxygen Intermediate (ROI) production of Â endothelialÂ cells was semiquantitatively measured by using NBT-reduction assay and the score of ROI was counted. The resultsÂ were statistically analyzed with ANNOVA (p<0.01). The rate of ROI production of endothelial cells was markedlyÂ increased after incubation with patient serum and neutrophils.
THEAFLAVIN MENGHAMBAT DIFERENSIASI PREADIPOSIT VISERAL MANUSIA MELALUI PPARÎ³ Î³Î³ Î³SECARA IN VITRO
Authors: Indra Rasjad
Published: January 2017

Differentiation of pre-adipocytes into adipocytes, is a complex process that involves coordination of Â expression of specificÂ genes and proteins associated with each stage of differentiation. This process is regulated by several signaling pathways.Â CCAAT element-binding proteins (C/EBP) Â Î±and Â Î²and sterol response element-binding protein 1 (ADD1/SREBP1) areÂ active during the early stages of the differentiation process and induce the expression and/or activity of the peroxisomeÂ proliferator-activated receptor Â Î³(PPARÎ³), a pivotal coordinator of adipocyte differentiation. Recently, many biologicalÂ effects of black tea have been reported and these effects may be partially related to its antioxidant Â properties. The recentÂ study showed that black tea flavonoids inhibited adipocyte differentiation. The aim of this study was to investigate the roleÂ of theaflavin, a flavonoids in black tea, in inhibiting adipocyte differentiation through inhibition of PPARÎ³expression.Â Preadipocytes were incubated in media containing standard-differentiated stimulator: Insulin, dexamethasone, IBMX andÂ FBS. After 24 hours, preadipocytes culture media wasadded with theaflavin 50, 100, or 200 Â ÂµM. The cells wereÂ harvested after TF incubation for 24 hours and then the expression of PPARÎ³was identified by immunocytochemistry andÂ Western blot.
PENGARUH PROBIOTIK PADA RESPON IMUN
Authors: Djunaedi Djoni
Published: January 2017

Probiotics (â€œfor lifeâ€) is a live microbial feed supplement or defined as mono- or mixed cultures of live microorganismsÂ which when administered in adequate amounts confer Â a health benefit to the host by improving its microbial balance.Â Common descriptives for probiotics include â€œfriendlyâ€, â€œbeneficialâ€ or â€œhealthyâ€ bacteria. Â Under natural conditions aÂ protective gut microflora develops and there is no Â need for probiotics supplement, but under rather un-natural conditionsÂ such like intestinal infections, post antibiotic treatment syndromes, pseudomembranous colitis, the probiotics preparationsÂ are needed. The beneficial effects of probiotics inthe host are promotion of the gut maturation and integrity, antagonismsÂ against pathogens and immune modulation. Beyond those, the microflora seems to play a significant rolein diet inducedÂ superinflammation, mucosal immune system, neuroendocrine regulation, immunoglobulin production, macrophages factorÂ restoration, apoptosis stimulation, lymphocyte function modulation, cytokine release, mucin production, intestinal immuneÂ homeostasis and inflammation prevention. However, many studies proved that probiotics have â€œupregulation and down-regulationâ€ effects on immune system of the host.
AKURASI METODE RADIOKOLLOID DAN BLUE DYEDALAM MENDETEKSI LIMFONODI SENTINEL PADA KANKER PAYUDARA STADIUM DINI
Authors: Yueniwati Yuyun , Yulisa N. Diana , Iskandar Arief
Published: January 2017

reast cancer incidence varies in many countries but in general increases. This research aimed to find Â out the accuracyÂ of radiocolloid (lymphoscintigraphy and gamma probe) as well as blue dye method in detecting sentinel node in the earlyÂ stage breast cancer. Diagnostic examination was conducted on 163 nodes of 14 patients cross sectionally, comparing theÂ accuracy of radiocolloid and blue dye methods with Â histopathologic method as the gold standard. Subject characteristicsÂ were female, aged 39-53, mostly menopause, multipara and breast-feeding. Primary tumors were mostly found in theÂ right, upper lateral quadrant, T2, histopathologic results showed that all were of invasive ductal carcinomas and 71.4%Â cases were lymphatic emboli. The detection of sentinel node with lymphoscintigraphy had 81.8% accuracy,sensitivityÂ 90.4%, specificity 73.2%, NPV 98.1%, false-negative1.8% and kappa value 0.37. Detection using gamma probe resultedÂ in 79% accuracy, sensitivity 90.4%, specificity 67.6%, NPV 97.9%, false-negative 2% and kappa value 0.31.
KADAR MDA DAN RASIO GSH/GSSH PADA KEHAMILAN NORMAL,PREEKLAMPSIA BERAT DAN EKLAMPSIA DI MALANG
Authors: Candra Siti , Widodo M Aris , Suwarto Soetomo , Muliartha G I Ketut
Published: January 2017

Preeclampsia is marked by vasospasme, increasing peripheral vascular resistance and permeability, endothelial cell andÂ reducing organ perfusion. It occurs because of the increasing of lipid hydroperoxides. The uncontrolled production of lipidÂ hydroperoxides causes oxidative stress and destroyscell integrity. Oxidative stress condition expressthe increasingÂ oxidant and decreasing antioxidant. One of the antioxidant is GSH that is used to neutralize the increasing ofÂ Malondialdehyde (MDA) level. The objective of this study was to see the difference of MDA content and GSH. GSSG ratioÂ between the normal pregnancy, preeclamsia and eclampsia. This study was cross sectional study. The samples were 36Â blood plasma consisted of 12 normal pregnancy, 12 preeclampsia and 12 eclampsia before birth. The bloodplasma, wasÂ measured for its MDA level and the GSH/GSSH ratio.The result showed that the level of MDA of plasma in preeclampticÂ and eclamptic pregnancy were higher than normal pregnancy p=0.000, due to lipid peroxidation resulted Â from increasedÂ production of lipid hydroperoxides on preeclamptic Â and eclamptic pregnancy.
AKTIVASI SPONTAN SEL T LIMFOSIT MENCIT YANG MENGALAMI DEFISIENSI IL2RÎ²
Authors: Endharti Agustina Tri
Published: January 2017

Homologous recombination applied to alter specific Â endogenous gene, referred as gene targeting, provides the highestÂ level of control over product mutation in cloned genes. In this experiment we showed that by using gene targeting a knockÂ out mutation in IL-2RÎ² gene could be generated. The findings of this study indicated that mice lacking the Interleukin-2Â receptor Î² chain (IL2RÎ²-/-) had spontaneously activated.T cells. In this study, we investigated abnormal expansion ofÂ activated T cells which showed high level of CD69. Â These findings suggest that the IL2RÎ²-/-Â mice may serve as animalÂ model for autoimmune diseases and that the accumulation of activated T cell in the future could be usedin clinical settingÂ to cure the diseases.Â Keywords:T cells, (IL2RÎ²-/-), autoimmune
PENGARUH LINGKUNGAN KERJA PERAWAT TERHADAP PELAKSANAAN PRAKTEK KEPERAWATAN DI RUMAH SAKIT UMUM Dr. SAIFUL ANWAR MALANG TAHUN 2006
Authors: Putra Kuswantoro Rusca , Hamid Achir Yani S. , Mustikasari Mustikasari
Published: January 2017

The implementation of nursing practice is related tonurses' working environment. The working environment componentsÂ consist of leadership, culture and management; control over workload; control over practice; and adequate source. TheÂ purpose of this study is to identify any correlation between the nurses work environment with the implementation ofÂ nursing practice. The research was conducted in IRNA(Inpatients Ward) I and of IRNA II RSU Dr. Saiful Â Anwar on MayÂ 1stuntil 20th, 2006. Correlation was chosen as the research design using cross sectional study on 196 nurses with twoÂ instruments: nurses' work environment, and implementation of nursing practice. The result based on analysis withÂ regression linier indicates that there is a significant correlation between working nurse environment(leadership, cultureÂ and management; control over workload; control overpractice; and adequate source) with the implementation of nursingÂ practice (p < 0.05) and control over practice is the biggest sub variable that influences the implementation of nursingÂ practice(B=1.302). In conclusion, the nurseâ€™s working environment influences implementation of nursingpractice inÂ hospital.Â Keywords:Work environment, Implementation of nursing practice
PENGEMBANGAN DIAGNOSIS TUBERKULOSIS PADA HEWAN KESAYANGAN ANJING MENGGUNAKAN ANTIGEN SPESIFIK Mycobacterium tuberculosisESAT-6 DAN CFP-10
Authors: Tjahajati Ida , Asmara Widya , Soebono Hardyanto
Published: January 2017

Early diagnosis is one of the important methods to Â control tuberculosis because this disease is zoonotic which easilyÂ spread through the air. Early detection of tuberculosis in dog is also very important since dog as petanimal have aÂ potency to transfer the disease to human or other animals. The discovery of two specific M.tuberculosisantigens,Â ESAT06 and CFP-10, provide the opportunity to developa specific diagnostic kit for tuberculosis by using ELISA basedÂ on the secretion of IFN-Î³. The development of a tuberculosis diagnostic kit based on this molecular biology andÂ immunological method would provide a good alternative method to detect tuberculosis specially, accurately as early asÂ possible. The result of this experiment would provide contribution for the development of health science and technology,Â especially in the eradication of tuberculosis.Â Keywords: diagnosis, tuberculosis, ESAT-6, CFP-10, interferon-gamma.
EFEK ANTIMIKROBA DARI EKSTRAK BIJI DAN DAUN MIMBA (Azadirachta indica A. Juss.) TERHADAP PERTUMBUHAN Malassezia globosa
Authors: Hastuti Utami Sri
Published: January 2017

Neem (Azadirachta indica A. Juss.) is a sort of a useful plant especially for health. Antimicrobial compounds in seed andÂ leaf of neem especially are: azadirachtin, fenol, quinon, alkaloid, nimbin, nimbidin and gedunin. Malassezia globosa is aÂ pathogenic yeast that cause dandruff on head skin. Â This research is done on behalf to know: 1) the inhibition of neemÂ seed extract to the growth of M. globosa in vitro;2) the difference of the effect of neem seed extract concentration to theÂ growth inhibition of M. globosa in vitro; 3) the Â inhibition of neem leaf extract to the growth of M.globosa; 4) theÂ difference of the effect of leaf neem extract concentration to the growth inhibition of M. globosa in Â vitro. Neem seed andÂ neem leaf extract made in some sort of concentration, i.e: 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%.Â The growth inhibition of M. globosa was measured by Â measuring the inhibition zone diameter of the yeastgrowth onÂ Sabouroudâ€™s Dextrosa Agar.
PENINGKATAN KUALITAS SPERMATOZOA PADA PROSES PEMISAHAN SPERMATOZOA DENGAN SENTRIFUGASI GRADIEN DENSITAS PERCOLL MELALUI PEMBERIAN FOSFOLIPID
Authors: Rumende Rooije R.H. , Kalim Handono , Widodo M Aris , Djati Moch Sasmito
Published: January 2017

The separation of X and Y spermatozoa can be conducted by using Percoll Gradient Density Centrifugation(SGDP)Â method. It is, easy, valid, cheap and applicable method for developing made-insemination. Although it Â has often beenÂ applied in sexing the spermatozoa, it was reported Â that the method had often caused damages on the spermatozoaÂ membranes and resulted in decreasing of the spermatozoa quality. The damages of spermatozoa after SGDP Â processÂ were specifically caused by (I) the loss of seminalplasma, (II) the increase of free radicals, and (III) the collision or frictionÂ among the spermatozoa. Among the three causal factors above, the third or the physical factor is claimed to be the mostÂ important one. It was assumed that the first and the second factors can be overcome if the third one is avoided. TheÂ spermatozoa membranes consist of lipids, protein, carbohydrate, and some substances at a low rate. ThegeneralÂ objectives of this research was to identify the influence of the phospholipids PC (Phosphatidylcholin)to spermatozoa inÂ order to avoid the damages of the spermatozoa in the process of SGDP.
RESISTENSI OBAT MALARIA: MEKANISME DAN PERAN OBAT KOMBINASI OBAT ANTIMALARIA UNTUK MENCEGAH
Authors: Simamora Dorta , Fitri Loeki Enggar
Published: January 2017

Multi drug resistance (MDR) of malariae parasite iscaused by decreasing ability of some antimalarial Â drugs examplesÂ chloroquine, mefloquine, antifolate and atovaquone Â to Plasmodium. Resistance to artemisin has not beenfound yet.Â Antimalaria drug resistance happened because some factors especially : genetic factor (gene mutation) Â of theÂ Plasmodium. Mechanism pathway of resistance differsin location, because the different target drug action. ResistanceÂ migh be reduce by using combination therapy.Â Key words: resistance, Plasmodium falciparum, antimalaria combination
PENGARUH PEMBERIAN PASTA TOMAT TERHADAP JUMLAH SEL BUSA PADA ARKUS AORTAAORTA ARCUS TUNICA INTIMA RATTUS NOVERGICUS STRAIN WISTAR TIKUS DENGAN YANG DIBERI DIET ATEROGENIK
Authors: Handayani Dian , Prijadi Bambang
Published: January 2017

In Indonesia, the prevalence of coronary heart disease (CHD) was increased. It currently became the main cause ofÂ mortality. Unhealthy dietary pattern as the most determinant of atherosclerosis through the increasingof plasma LDL, willÂ be worsen by the presence of free radical such as reactive oxygen species (ROS) that led to accumulation ofÂ atherosclerosis plaques. The negative effects of free radicals can be inhibited by antioxidant. Lycopene is a strongÂ antioxidant that able to neutralize free radicals, Â especially oxygen derived free radicals. This studyaimed to assess theÂ effect of tomato paste containing lycopne to total Â foam cells. Lycopene has the role to prevent artericoronaria related toÂ atherosclerosis. The most lycopene source is tomatoproducts. This study was a laboratory-experimental study withÂ randomized control group â€“ post-test design. Twentyrandomly selected rats, divided into 5 Â treatment groups, group P1Â (normal diet treatment), P2 (atherogenic diet treatmen)t, P3 (atherogenic diet and addition 0.01 g/days oftomato pastaÂ treatment, P4 (atherogenic diet and addition 0.02 g/days of tomato pasta treatment,),
IDENTIIFKASI PROTEIN IMUNOGENIK CHLAMYDIA PNEUMONIAE TERHADAP SERUM PENDERITA INFARK MIOARD AKUT
Authors: Murwani Sri , Hidayati Dwi Yuni Nur
Published: January 2017

Chlamydia pneumoniae is human respiratory tract pathogen and recently investigated as pathogen causingatherosclerosis and acute myocardial infarction (AMI). This research was carrier out to detect proteinpattern of C.Â pneumoniae, and to study it relation to AMI throughdetection of immunogenic protein. Design research Â was laboratoryÂ observational and analyzed descriptively. The subject was C. pneumoniae. Protein pattern the bacteria was detected byÂ electrophoresis method, and to detect the immunogenic protein was done immunoblotting. Serum was obtained from AMIÂ patients in Saiful Anwar and Lavallette hospitals. Â The result showed, protein pattern C. pneumoniae wasprotein withÂ molecular weight 117, 107, 97, 91, 86, 61, 58, 52, 46, 44, 34, 23, 19, 9, 5, 4 kDa. Immunogenic proteins vary betweenÂ AMI patients was 117, 107, 86, 61, 58, 52, 46, 44, Â 34 kDa. Non immunogenic proteins were 97, 91, 23, 19, 9, 5 and 4Â kDa. Protein 61 kDa react to all of patientâ€™s serum. It was concluded, C. pneumoniae have protein fractions 117, 107, 97,Â 91, 86, 61, 58, 52, 46, 44, 34, 23, 19, 9, 5, and 4kDa. Immunogenic proteins vary between AMI patients was 117, 107,Â 86, 61, 58, 52, 46, 44, 34 kDa, and 61 kDa was the Â immunodominant protein. The result proved C. pneumoniae asÂ causative agent of atherosclerosis and acute myocardial infarction, in Indonesia particularly.Â Key words:C. pneumoniae, immunogenic, AMI
PENGARUH STRESOR PSIKOLOGIK TERHADAP DISTRIBUSI TRANSPORTER SEROTONIN (SERT) DAN INDEKS APOPTOSIS HIPOKAMPUS YANG DIMEDIASI OLEH KORTISOL DAN IL â€“ 6
Authors: Suparno Suparno
Published: January 2017

Generally, distress which followed by psychiatric disorders still become a medicinal practice controversial especiallyÂ psychologically stressor roles on holistic health condition. Therefore, research is needed to explain and clarify theÂ influences and the role of psychologically stressors on psychiatric disorders. This study used an psychoneuroimmunologicÂ paradigm, which used posttest-only true experiment Â design. About 24 female Rattus norvegicus strain Â Wistar had beenÂ used, divided into six groups. To investigate functional changes, we used hippocampus SERT distribution Â changes asÂ mirror of central serotonergic malfunction changes.The structural changes was measured by apoptosis index ofÂ hippocampus CA3 regions (hippocampus slicing). We also took the blood from the rats heart to examine Â IL â€“ 6 andÂ cortisol level.Â Psychological stressors lead to measuring of functional and structural changes include hippocampus SERT distributionÂ and increase hippocampus apoptotic index, that finally arise the distress.These results of statistical analyses demonstrateÂ that psychological stressors seem to be mediated by serotonergic neurotransmission pathway.Â Key words :hippocampus CA3 region, SERT malfunction, IL-6, cortisol, psychological stressors
FAKTOR YANG DIDUGA MENJADI RESIKO PADA ANAK DENGAN RINITIS ALERGI DI RSU DR. CIPTO MANGUNKUSUMO JAKARTA
Authors: Harsono Ganung , Munasir Zakiudin , Siregar Sjawitri P , Suyoko HEM Dadi , Kumiati Mia , Evalina Rita , Palupi Ratih D
Published: January 2017

This researach was aimed to describe the risk factor profile of pediatric patients with allergic rhinitis. 1792 medical recordsof outpatients pediatric allergy immunology clinic in Cipto Mangunkusumo Hospital from 1997 to 2005. Fifty patients wereÂ diagnosed with allergic rhinitis and 22% of them Â were having allergic rhinitis with bronchial asthma.Allergic rhinitis wereÂ identified higher in boys (62%) than girls (38%) and ranged from 5 months to 13 years and 8 months old. Atopic historyÂ were identified in 24 patients (48) while atopic history in patient family were identified in mother (42%), father (40%), andÂ siblings (24%). Total IgE serum increased in 35 patients (88,57%) while total eosinophil serum increased in 28 patientsÂ (80%). The most common aeroallergen by skin prick test was house dust mite (36%) and the most common food allergenÂ was shrimp (40%). There were several factors that contribute to the development of allergic rhinitis such as age, sex,Â family atopic history and increasing in total IgE serum and total eosinophil serum . House dust mite and shrimp were theÂ most common allergen identified in allergic rhinitis.Â Keywords: allergic rhinitis, risk factors, pediatric
CYCLOOXYGENASE-2 (COX-2) EXPRESSION ON TESTIS CONNECTIVE TISSUE OF Rattus norvegicusAFTER TREATMENT WITH BETEL NUT EXTRACT (Areca catechu)
Authors: Akmal Muslim , Riawan Wibi
Published: January 2017

Betel nut contains alcaloids such as arecoline, arecaine, arecaidine, arecolidine, guvacine, guvacoline and isoguvasine.Â Arecoline has ability to change gonad morph-function, including shape abnormality of sperm. This research was conductedÂ to prove the ability of betel nut extract (Areca catechu) in causing apoptosis on testis connective tissue of RattusÂ novergicus. This research used male; 2-3 months age, 150-200 grams body weight of white rats Rattus norvegicus strainÂ Wistar. The rats were divided into 5 groups in equalnumber, 3 rats respectively. They were a control group withoutÂ treatment and 4 groups as treatment groups which were given doses of betel nut extract, i,e., 1, 2, 3 Â and 4 gram duringÂ seven days. The result showed that dose variation ofbetel nut extract could induction of COX-2 expression on rats (RattusÂ norvegicus) strain Wistar testicular seminiferous tubule.Â Keywords: Betel nut extract, Rattus norvegicus, testis, cyclooxygenase-2
POTENSI Musca domesticaLinn. SEBAGAI VEKTOR BEBERAPA PENYAKIT
Authors: Hastutiek Poedji , Fitri Loeki Enggar
Published: January 2017

Musca domestica is a fly which can be found aroundhouse and cage. Its present interference human hygiene, calmnessÂ and health. This fly generating many loss at human being and animal because this fly have high potency Â in spreadingÂ many diseases agents, that are protozoa, worm, virus, bacterium and fungi. Several agents of emerging,reemerging andÂ new emerging diseases can be transferred by M. domestica such as Cryptosporidium parvum, Helicobacter pylori,Â E.coli O157:H7 and H5N1. Agens removed by M. domestica through regurgitasi, excrete and exoskleleton. InterestinglyÂ M. domestica might be act as biological vector.Â Key words: Musca domestica, agent of disease
TGF-Î²MERUPAKAN MEDIATOR IMUNOSUPRESI DARI SEL T CD4 + CD25 + IN VITRO
Authors: Endharti Agustina Tri
Published: January 2017

Naturally the existency of regulatory T cells are represented by CD4+CD25+T cells. Those cells have been identified as aÂ population of immuno-regulatory T cells, which mediated suppression of CD4+CD25+T cells by cellâ€“cell contact. In theÂ effectors phase the function of CD4+CD25+T regulatory T cells through the involvement of certain suppressive cytokines,Â such as TGF-Î². In this study, we demonstrated that CD4+CD25+Â cells produced high levels of Transforming GrowthÂ Factor-Î² (TGF-Î²) compared with CD4+CD25-T cells when stimulated by plate-bound anti-CD3 andsoluble anti-CD28. WeÂ observed that suppression ability of those cells could be abolished by the presence of antiâ€“TGF-Î². Finally, we found thatÂ stimulated CD4+CD25+T cells but not CD4+CD25-T cells express high and persistent levels of TGF-Î². This finding stronglyÂ suggests that CD4+CD25+T cells exert immuno-suppression by cellâ€“cell surfaces interaction involved cell of TGF-Î².Â Keyword: Sel T CD4+CD25+, TGF-Î², immunosupresion
PENGARUH PEMBERIAN KOMBINASI NAC DENGAN VITAMIN C DAN E TERHADAP STRES OKSIDATIF PADA HUVECs DIPAPAR PLASMA EKLAMPSIA
Authors: Baktiyani Siti Candra Windu
Published: January 2017

The blood plasma of preeclampsia contains oxidative stress factors. The oxidative stress is predicted asthe cause of dis-function and destruction of endothelial cell which will influence the severity of Preeclampsia and Eclampsia complications.Â Previous research showed that the treatment of antioxidant on preeclamsia was still controvertion. The Â objectives of thisÂ research is to investigate the influence of the combination of NAC and vitamin C or vitamin E or bothof them on the levelÂ of H2O2, MDA, NO and GSH/GSSG ratio of endothelial cell culture exposed with Eclampsia plasma.The method wasanÂ experimental study using HUVECs exposed with to 2%plasma Eclampsia as an Eclampsia model invitro. TheHUVECsÂ were than treated with NAC 2ÂµM only (group 1), NAC2ÂµM + vitamin C 100 ÂµM (group 2) , NAC 2 ÂµM + vitamin E 100Â ÂµM (group 3), and NAC 2 ÂµM + vitamin C 100 ÂµM + vitamin E 100 ÂµM (group 4) for 24 hours. The parameter measuredÂ in this study were H2O2, MDA, NO and GSH/GSSG ratio. Result showed that H2O2, MDA, and NO concentration areÂ unsignificantly different among treatment groups although there was a decreasing on group treated with combination ofÂ NAC, vitamin C and Vitamin E.
LAPORAN KASUS: PERBANDINGAN PROSEDUR CUTLER BEARD DAN DIRECT CLORUSE PADA DEFEK PALPEBRA YANG LUAS AKIBAT TRAUMADI RSU DR. SAIFUL ANWAR MALANG
Authors: Kurniawan M. Iwan , Sadono Elfina G
Published: January 2017

The paper aimed to report a different management ofreconstruction for large traumatic upper eyelid defect. Case 1 : aÂ 17 years old woman with traumatic upper eyelid defect in the right eye since 2 years old. The defect was linear but wide,Â looks like a triangle defect. Defect had repaired by direct closure. After the operation the defect remain. Case 2 : a 45Â years old man with more than 50 % traumatic upper eyelid defect in the left eye. Cutler-beard procedurwas made toÂ close the defect. Separation of the flap had been done at the second procedure. Cutler-beard procedure, done to theÂ second patient, had succed to close the upper eyelid defect. Reconstruction procedure carried out for Â the first patientÂ was not adequate to restore the large upper eyelid Â defect. Cutler-beard procedure is used for upper eyelid defectÂ involving more than 50% seem to have a good result.Key words: Cutler beard, Direct closure
PENGARUH PEMBERIAN MINYAK BUAH MERAH (Pandanus conoideus oil) TERHADAP PEMBENTUKAN FOAM CELLPADA AORTA TIKUS GALUR WISTAR (Rattus norvegicus) DENGAN DIET ATEROGENIK
Authors: Syarkiah Syarkiah , Fitri Loeki Enggar , Pudjirahaju Astutik
Published: January 2017

Coronary heart disease serves as the main cause of death in the world. One of the effort to reduce therisk ofÂ coronary heart disease is by consuming food containing antioxidant. Red fruit contains high antioxidant,Â namely Î²-carotine (700 ppm) and alphatocopherol (500 ppm). This study was intended to find out the effect ofÂ red fruit oil (Pandanus conoideus oil) toward the formation of foam cell in wistar rat (Rattus norvegicus) withÂ atherogenic diet.This study was an experimental study, the design applied was complete randomized designÂ with 3 repetitions. The treatment in this study wasthe addition of red fruit oil in standard diet andatherogenicÂ one, comprising 8 groups, namely P0group (negative control), P1group (positive control), P2group (standardÂ diet + red Fruit Oil 0,12 ml/day), P3group (standard diet + red Fruit Oil 0,24 ml/day),P4group (standard diet +Â red Fruit Oil 0,36 ml/day), P5group (atherogenic diet + red Fruit Oil 0,12 ml/day), P6group (atherogenic diet +Â red Fruit Oil 0,24 ml/day), and P7group (atherogenic diet + red Fruit Oil 0,36 ml/day). The parameter was theÂ number of foam cells. The process and data analysisapplied Oneway Anova statistical test, followed withÂ DMRT (Duncan Multiple Range Test) statistical test.The result of Oneway Anova statistical test indicated thatÂ there was a significant difference of foam cell number among groups (p = 0,000). The conclusion inferredÂ from this study is that the addition of red fruit oil with dosage 0.24 ml/day and 0,36 ml/day may significantlyÂ obstruct the formation of foam cell in wistar strain rat with atherogenic diet.
PREVALENSI DEPRESI DAN GAMBARAN STRESSOR PSIKOLOSOSIAL PADA REMAJA SEKOLAH MENENGAH UMUM DI WILAYAH KOTAMADYA MALANG
Authors: Asmika Asmika , Harijanto Harijanto , Handayani Nina
Published: January 2017

During the mental development process, the adolescent will be exposed tonumerous events that might act asÂ psychosocial stressors. These stressors might contribute to the prevalence of depression in adolescent. ThisÂ study aimed to determine the prevalence of depression description of psychosocial stressor, and theÂ relationship between psychosocial stressors and depression in adolescent. A cross sectional descriptiveÂ analytic study was conducted using 458 particiapants from three representative Senior High Schools inÂ Malang district. The depression level was measured Â using Beck Depression Inventory while Holmes andÂ Rahe Stress Scale for Youth was used to mesure the Â level of psychosocial stressor. It was found thatÂ prevalence of depression were as follows 32,5% of respondents with mild depression; 28,2% with moderateÂ depression; and the remaining 11,1% withsevere depression. Of all particiapants, 59,6% experience a highÂ level stressor, while the other 40,4% have low level stressor. The study identify a significant correlationÂ (p<0.001)between depression level and level of psychosocial stressor. . The risk of having depression is 5.87Â higher in respondents with high level of psychosocial stressor compare to those with low level psychosocialÂ stressor (OR=5.87). Therefore, a cooperation between school, community and parent is highly advised toprovide a favourable environment for mental development in adolescent. In addition parents, school need toÂ prepare the adolescent in order to anticipate numeros events that influence their mental health.
KEMAMPUAN DARI Lactobacillus plantarum GALUR IS-10506 DAN IS-20506 DALAM MENGHAMBAT AKTIVASI NFkB, MEREGULASI TURUN TNF-RECEPTOR 1 (TNF-R1) DAN OPOPTOSIS PADA BRUSH SEL EPITEL BORDER Rattus novergicus YANG DIINDUKSI LPS
Authors: Kusuma Titis Sari , Riawan Wibi , Gunadi Ranuh I Gusti Made Reza , S. Surono Ingrid
Published: January 2017

Probiotic bacteria have a beneficial effect on diarrhea. In this study, we have examined effect of LactobacillusÂ plantarum Strain IS-10506 (LIS-10506) and IS-20506 Â (LIS-20506) on inhibition of NFÎºB activation,Â downregulation TNF Receptor-1 (TNF-R1) and Apoptosis in Epithelial Brush Border of Rattus novergicusÂ induced with LPS. On the first day, LPS was inducedto Rattus novergicus per oral. At the 3rdday,Â Lactobacillus plantarum Strain IS-10506 and LIS20506 separately were supplemented for 7 days (the 3rddayÂ up to the 9thday). Rat was sacrificed after anaesthetizing with ether to assess NFÎºB activation, TNFR1 andÂ apoptosis. Result showed the decreases of activation NFÎºB in LIS-10506 group as well as in LIS-20506Â group, significantly different, in NFÎºB activation at group with only LPS (average 14.33+4.509) compared toÂ group with LIS-10506 induction (average 2.00+1.732)and LIS-20506 induction (average 1.33+1.528), at p(Â p<0.000). Downregulation of TNF-R1 was significant Â at LPS group compared to LIS-10506 induction as wellÂ as LIS-20506 induction. The index of apoptosis showed significant of degradation (p<0.000) after induced byÂ LIS, where LPS group (14.67+2.517), LIS-10506 induction (6.33+2.309) and LIS-20506 inductionÂ (6.00+3.000). As a conclusion, supplementation of LIS-10506 and LIS-20506 separately will inhibit theNFÎºBÂ activation, and although the mechanism was not sure, what by significant degrade the expression TNF-R1(Â as ekuivalen of activity TNFÎ±), and the mentioned give the implication that happened by the degradation ofÂ occurence apoptosis at cell of epitel bush border intestine.
PROFIL KADAR SOLUBLE UROKINASE PLASMINOGEN ACTIVATOR RECEPTOR (suPAR) PADA SERUM PENDERITA TUBERKULOSIS PARU (SEBAGAI MONITORING TERAPI)
Authors: Astuti Triwahju , MR Tri Yudani , Riawan Wibi , Muktiati Nunuk Sri , Widjajanto Edy
Published: January 2017

Dutch study shows that Upar expression content is significantly higher in tubercolusis patientâ€™s psiphsyÂ monosit compared to those in the healthy control group. So far, there is no biologic marker used whichcanÂ accurately observe response improvement in the treatment of lung tubercolusis. The aim of this research is toÂ investigate whether the serum level of soluble utokinase plasminogen activator receptor (suPAR) carriesÂ information in monitoring TB treatment for Lung Tuberculosis patients. suPAR was measured by ELISA in 21Â individuals at the time of enrolment into observational cross sectional based on active tuberculosis Â and 5Â individuals as healthy control. There were 3 groups, 1). patients who had not started treatment (n=7),2).Â patients who had been treated for 2 months (n=7), Â 3). patients who had been treated for 6 months (n=7).Â Among groups, there were no difference in mean of body mass index, erythrocyte sedimentation rate andÂ monocyte count. Patients positive for TB on direct Â microscopy were 29% ( 6 from 21 patients) , 2 patientsÂ each groups. The suPAR levels were elevated in patients with active TB compared to healthy controlÂ (P<0,001). suPAR levels were highest in patients positive for TB on direct microscopy ( mean suPAR 4.455Â ng/ml).
PERAN LEPTIN TERHADAP AKTIVITAS MATURATION-PROMOTING FACTOR(MPF) PADA MATURASI OOSIT
Authors: Kakisina Pieter , Indra Rasyad
Published: January 2017

Oocyte maturation is mediated by active Maturation Â Promoting Factor (MPF) in ooplasma. MaturationÂ Promoting Factor (MPF) is heterodimer protein consists of sub catalytic unit p34cdc2 and sub regulatorunit ofÂ cyclin-B. The regulation of maturation promoting factor (MPF) occures throught phosphorylation andÂ dephosphorilation Cdc2 while its activities are controlled by the accumulation and periodical degradation fromÂ Cyclin-B during cell cycles development. Leptin inknown has role on oocyte maturation that works atÂ mitogen activated protein kinase (MAPK). MAPK belongs to protein kinase family Ser/Thr that interact withÂ MPF in arranging the progresivennes of oocyte meiosis through the help of c-mos. This interaction is initiatedÂ phosphorilation of Ser-369 and Thr-577 in p90rsk.Â Key words:leptin, MPF, and oocyte maturation.
ARTERIOGENESIS DAN ANGIOGENESIS PADA STROKE HEMORAGIK:MEMPERTAJAM KONSEP UNTUK MEMPEROLEH MANFAAT TERBAIK NEOVASKULARISASI *
Authors: Lyrawati Diana
Published: January 2017

Arteriogenesis and angiogenesis contribute both to Â the damage and repair in hemorrhagic stroke.Â Understanding and unraveling the fundamental and mechanisms of these processes should facilitate theÂ development of treatment strategies to improve outcome after stroke. This article describes briefly the basicÂ principles of arteriogenesis and angiogenesis completed with some key molecular players, followed by aperspective of the author on how to use those neovascularization to benefit repair and recovery processes inÂ post-hemorrhagic stroke.Â Key Words : arteriogenesis, angiogenesis, hemorrhagic, stroke
HASIL SATU TAHUN INTERVENSI JARINGAN PENANGGULANGAN TUBERKULOSIS PARU KECAMATAN ILIR BARAT II KOTA PALEMBANG
Authors: Tjekyan R.M. Suryadi
Published: January 2017

AsÂ  ofÂ  nearlyÂ  theÂ  endÂ  ofÂ  2007,Â  NationalÂ  TuberculosisÂ  ProgramÂ  haveÂ  notÂ  achievedÂ Â Â  MDGâ€™SÂ  indicatorÂ  timely especiallyÂ  inÂ  SouthÂ  Sumatera.Â  ThisÂ  wasÂ  causedÂ  byÂ  LocalÂ  andÂ  NationalÂ  TuberculosisÂ  ControlÂ  Program administeredÂ  merelyÂ  byÂ  governmentsÂ  withÂ  minimalÂ  privateÂ  sectorÂ  andÂ  communityÂ  participation.Â  AÂ  NetworkÂ  of Pulmonary Tuberculosis Eradication Program was established in Ilir Barat II District of South Sumatera. This networkÂ  isÂ  directedÂ  byÂ  fourÂ  componentsÂ  ofÂ  TBÂ  control:Â  cadres,Â  PhysicianÂ  Practitioner,Â  HeadÂ  ofÂ  subdistrict, district local authority, and district tuberculosis program. The Network aims at accelerating TB Contol Program since first October 2006 until first October 2007. The final goal is to have â€œDistrict Free TBâ€. Theâ€Networkâ€ was foundedÂ  byÂ  decreeÂ  ofÂ  theÂ  MayorÂ  ofÂ  IlirÂ  BaratÂ  IIÂ  DistricÂ  .Â  TheÂ  resultÂ  ofÂ  oneÂ  yearÂ  implementationÂ  indicated significant increased of the Indicator of TB program, more than the MDGâ€™starget.Â  If the networkÂ  is applied at PalembangÂ  cityÂ  andÂ  allÂ  SouthÂ  Sumatera,Â  thoseÂ  areasÂ  willÂ  reachÂ  100%Â  conversionÂ  rateÂ Â Â  byÂ  theÂ  endÂ  ofÂ Â Â  2008 and 100% case detection rate by the end of 2010. Â Keywords:Â  TuberculosisÂ  ControlÂ  Program,Â  NetworkingÂ  Model,Â  HealthÂ  Cadre,Â  PrivateÂ  Physician,Â  LocalÂ  Government, Millenium Development Goalâ€™s
DISTRIBUSI MAST CELL PADA MESENTERIUM TIKUS
Authors: N Ibrahim Mohamad , Widjajanto Edi , Rosita Rita
Published: January 2017

Mast cell are derived from haematopoietic progenitors in the bonemarrow. Developing mast cell subsequently migrate to peripheral tissues, such as the skin, mucosa and air way, where they terminate their differentiation underÂ  theÂ  influenceÂ  ofÂ  factorsÂ  inÂ  theÂ  tissueÂ  micro-enviroment.Â  ThisÂ  studyÂ  aimedÂ  toÂ  knowÂ  distributionÂ  and strucutre of mast cell on rat mesentery. The mesentery was stained with a 1 % safranine solution or toluidine blueÂ  onÂ  theÂ  aÂ  peparedÂ  slide.Â  TheÂ  preparedÂ  slidesÂ  wereÂ  immediatelyÂ  viewedÂ  byÂ  aÂ  observerÂ  throughÂ  a microscope.Â  TheÂ  resultÂ  showedÂ  thatÂ  mastÂ  cellsÂ  stainedÂ  withÂ  safranineÂ  orÂ  toluidineÂ  blueÂ  wereÂ  closely associated with postcapillary in the rat mesentery. Keywords : mast cell, mesentery
PENGARUH PEMBERIAN CORNMEAL DAN CORNMEAL-SOY TERHADAP KADAR KOLESTEROL DALAM SERUM TIKUS
Authors: Harti Leny Budhi , Rudijanto Achmad , Kristianto Yohanes
Published: January 2017

CoronaryÂ  HeartÂ  DiseasesÂ  (CHD)Â  areÂ  aÂ  seriousÂ  healthÂ  problemÂ  thatÂ  commonlyÂ  occurÂ  notÂ  onlyÂ  inÂ  modern countries, but asloÂ  in developing countries likeÂ  Indonesia. The high cholesterol level inÂ  blood isÂ  a risk factor thatÂ  mayÂ  causeÂ  CHDÂ  byÂ  atherosclerosisÂ  mecanism.Â  dietaryÂ  fiberÂ  mayÂ  beÂ  usedÂ  toÂ  preventÂ  atherosclerosis. Cornmeal and cornmeal-soy are products of corn, that rich in fiber. This research was aimed to study about theÂ  effectÂ  ofÂ  cornmealÂ  andÂ  cornmeal-soyÂ  onÂ  serumÂ  cholesterolÂ  levelÂ  ofÂ  6-8Â  mounthsÂ  maleÂ  wistarÂ  rats.Â  The subjectsÂ  areÂ  25Â  maleÂ  wistarÂ  rats.Â  TheseÂ  miceÂ  willÂ  beÂ  providedÂ  intoÂ  fiveÂ  groups.Â  (1)Â  gruopÂ  thatÂ  given atherogenicÂ  dietÂ  only,Â  (2)Â  gruopÂ  thatÂ  givenÂ  atherogenicÂ  dietÂ  withÂ  cornmealÂ  3.4Â  g/day,Â  (3)Â  gruopÂ  thatÂ  given atherogenic diet with cornmeal 6.8 g/day, (4) gruop that given atherogenic diet with cornmeal-soy 3.6 g/day, (5)Â  gruopÂ  thatÂ  givenÂ  atherogenicÂ  dietÂ  withÂ  cornmeal-soyÂ  7.2Â  g/day.Â  DataÂ  collectingÂ  includeÂ  firstÂ  weight,Â  last weight,Â  weightÂ  gain,Â  nutrientÂ  intake,Â  andÂ  serumÂ  lipid.Â  StatisticÂ  analysisÂ  thatÂ  beÂ  usedÂ  isÂ  ANOVAÂ  oneÂ  way. Results showed thatÂ  wistar rats which given atherogenic diet with cornmeal and cornmeal-soyÂ  had trend to increaseÂ  cholesterolÂ  andÂ  LDLÂ  level.Â  AccordingÂ  toÂ  statisticÂ  analysis,Â  cholesterolÂ  andÂ  LDLÂ  levelÂ  amongÂ  groups were not significant (p > 0.05). However wistar rats which given atherogenic with cornmeal and cornmeal-soy inÂ  highÂ  doses,