bluuebunny/crossref_metadata_2025 huggingface dataset:
Subsea Power Cable Health Management Using Machine Learning Analysis of Low-Frequency Wide-Band Sonar Data
Authors: Tang Wenshuo , Brown Keith , Mitchell Daniel , Blanche Jamie , Flynn David
Published: August 2023

Subsea power cables are critical assets for electrical transmission and distribution networks, and highly relevant to regional, national, and international energy security and decarbonization given the growth in offshore renewable energy generation. Existing condition monitoring techniques are restricted to highly constrained online monitoring systems that only prioritize internal failure modes, representing only 30% of cable failure mechanisms, and has limited capacity to provide precursor indicators of such failures or damages. To overcome these limitations, we propose an innovative fusion prognostics approach that can provide the in situ integrity analysis of the subsea cable. In this paper, we developed low-frequency wide-band sonar (LFWBS) technology to collect acoustic response data from different subsea power cable sample types, with different inner structure configurations, and collate signatures from induced physical failure modes as to obtain integrity data at various cable degradation levels. We demonstrate how a machine learning approach, e.g., SVM, KNN, BP, and CNN algorithms, can be used for integrity analysis under a hybrid, holistic condition monitoring framework. The results of data analysis demonstrate the ability to distinguish subsea cables by differences of 5 mm in diameter and cable types, as well as achieving an overall 95%+ accuracy rate to detect different cable degradation stages. We also present a tailored, hybrid prognostic and health management solution for subsea cables, for cable remaining useful life (RUL) prediction. Our findings addresses a clear capability and knowledge gap in evaluating and forecasting subsea cable RUL. Thus, supporting a more advanced asset management and planning capability for critical subsea power cables.
Leakage Monitoring and Quantitative Prediction Model of Injection–Production String in an Underground Gas Storage Salt Cavern
Authors: Jiang Tingting , Cao Dongling , Liao Youqiang , Xie Dongzhou , He Tao , Zhang Chaoyang
Published: August 2023

The leakage of the injection–production string is one of the important hidden dangers for the safe and efficient operation of underground salt cavern gas storage. Although distributed fiber optic temperature measurement system (DTS) can accurately locate the position of the string leakage port, how to establish the quantitative relationship between the temperature difference and leakage rate of the leakage port still needs further exploration. This paper proposes a new quantitative prediction model based on a DTS for the leakage monitoring of the injection–production string of salt cavern gas storage. The model takes into account the gas’s physical parameters, unstable temperature conditions, and the Joule–Thomson effect. In order to verify the accuracy of the model, a simulation experiment of string leakage based on a DTS was carried out. The test results show that the relative deviation between the predicted leakage rate and the measured value is less than 5% compared with the calculated value. When the leakage rate drops to 0.16 m3/h and the temperature range is less than 0.5 °C, it is difficult to accurately monitor the DTS. The results of this study help to improve the early warning time of underground salt cavern gas storage string leakage.
Seamless Start-Up of a Grid-Connected Photovoltaic System Using Module-Integrated Micro-Converters
Authors: Callegaro Leonardo , Uong Trung-Hieu , Deilami Sara
Published: August 2023

In traditional grid-tied photovoltaic (PV) installations, when partial shadowing occurs between different PV modules in a string, bypass diodes short-circuit the output terminals of shadowed modules, and the whole system forgoes their potential energy production. This loss can be recovered if a dc-dc converter (micro-converter) is coupled to every PV module, and operated at the maximum power point (MPP). In this scenario, without communication links between the distributed micro-converter and the grid-tied inverter, a start-up procedure must be carefully designed to seamlessly allow the system to transfer PV power to the grid. During this phase, potentially damaging over-voltages and abrupt transients occurring at the micro-converters/inverter interface must be avoided. In this paper, the control algorithm of each micro-converter is enhanced to provide a smooth start-up operation so that PV units can safely start transferring power to the inverter and the grid. Improving from previous works, the proposed control technique is simple and removes the need for current sensors at the output of each micro-converter and at the inverter dc-link, with an economical advantage. Simulation results demonstrate the successful system start-up behavior, whilst confirming the benefits of the proposed control technique. First, the dc-link is energized from the rectified grid voltage. Then, the micro-converters raise the dc-link voltage so that the available PV power is transferred to the grid, with this sequence of operations not causing any abrupt electrical transient. The results also demonstrate the robust behavior of the PV system under non-uniform solar irradiation conditions.
Strategic Analysis of the Renewable Electricity Transition: Power to the World without Carbon Emissions?
Authors: Thompson Shirley
Published: August 2023

This paper explores the role of electricity in the transition to renewable energy to mitigate climate change. A systematic literature review with the Scopus database identified 92 papers relevant to the renewable electricity transition. A PESTLE (Political, Economic, Sociological, Technological, Legal, and Environmental) review of the papers provided a multidisciplinary analysis. The Paris Agreement created a global movement for carbon neutrality to address the threats of climate change, calling for a transition to renewable electricity to lead the way and expand into new sectors and regions. Although smaller renewable technologies are ramping up, complexities thwarting the transition include locked-in assets, high upfront costs, variability of solar and wind energy, infrastructure, difficulty in decarbonizing transportation and industry, material resource constraints, and fossil fuel support. This research found that renewables are not replacing fossil fuels to date but adding further energy demands, so that greenhouse gas emissions rose in 2021 despite an increased renewable electricity share. Without a major shift in the trillions of dollars of subsidies and investment away from fossil fuels to renewables, catastrophic climate change is predicted. This paper found that the Paris Agreement’s commitment to net-zero carbon and the transition to renewable electricity are undermined by record-high levels of subsidies and financing for fossil fuel industry expansion. Transitioning to a climate-neutral economy requires an investment away from fossil fuels into renewable energy ecosystems. Renewable electricity provides possibilities to realize sustainable development goals, climate stabilization, job creation, a green economy, and energy security with careful planning.
The Interplay of Green Technology and Energy Consumption: A Study of China’s Carbon Neutrality and Sustainable Digital Economy
Authors: Alam Isbat , Lu Shichang , Sarfraz Muddassar , Mohsin Muhammad
Published: August 2023

In light of the carbon neutrality goals set post-Paris Climate Conference (COP21), this study delves into the relationship between green technology innovations, energy consumption, and CO2 emissions in China, spanning the period of 1990 to 2021. The objective of this paper is to creatively present the idea of a low-carbon digital economy from the viewpoint of digital technology. Utilizing the Stochastic Impacts by Regression on Population, Affluence, and Technology (STIRPAT) model, we scrutinize this relationship, employing unit-root testing to verify the integrative attributes of the variables, inclusive of structural break data. Further analysis using the bootstrap Autoregressive Distributed Lag (ARDL) bound testing method corroborates the relationship between these key variables. The study reveals unidirectional co-integration over time among green technology innovations, renewable and non-renewable energy, per capita income, population, and CO2 emissions as per the Granger causality test. Interestingly, our findings suggest that while green technology innovation, per capita income, and renewable energy contribute to the reduction of CO2 emissions, non-renewable energy consumption and population growth exacerbate them. These insights offer valuable guidance for policymakers in formulating comprehensive strategies to enhance environmental quality through the promotion of renewable energy and green technology innovations, with a specific emphasis on the Chinese context.
Reaction Molecular Dynamics Study on the Mechanism of Alkali Metal Sodium at the Initial Stage of Naphthalene Pyrolysis Evolution
Authors: Wu Di , Dong Heming , Luan Jiyi , Du Qian , Gao Jianmin , Feng Dongdong , Zhang Yu , Zhao Ziqi , Li Dun
Published: August 2023

In order to study the role of metal sodium in the spray pyrolysis of biomass tar, this paper designs a sodium-containing naphthalene pyrolysis system (NSS) and a pure naphthalene pyrolysis system (PNS) using naphthalene as the carbon source and sodium chloride as the sodium metal donor for comparison. This enables an exploration of the effect of sodium on the initial nucleation of carbon fumes formed by naphthalene pyrolysis using reaction molecular dynamics (ReaxFF MD). The simulation results show that NSS undergoes pyrolysis reactions earlier and faster than PNS at the same temperature. Simulated at 3250 K temperature for 2 ns, the naphthalene pyrolysis consumption rate of the NSS was faster than that of the PNS, and the addition of sodium atoms during the condensation process provided more active sites and accelerated the condensation of macromolecular products. Moreover, Na+ and carbon rings form a Na+-π structure to promote the bending of graphite lamellae to facilitate the formation of carbon nuclei. Molecular dynamics simulations were used to simulate the formation of carbon nuclei during the initial stage of naphthalene pyrolysis, revealing that the mechanism of sodium salt catalyzed the acceleration of organic matter pyrolysis from a microscopic visualization perspective.
Analysis of Voltage Distortions in the Power Grid Arising from Agricultural Biogas Plant Operation
Authors: Kuboń Maciej , Skibko Zbigniew , Tabor Sylwester , Malaga-Toboła Urszula , Borusiewicz Andrzej , Romaniuk Wacław , Zarajczyk Janusz , Neuberger Pavel
Published: August 2023

Agricultural biogas plant operations are energy sources that fit well with rural land use. The continuous increase in cattle and pigs creates the need to manage animal waste, especially slurry. Using it for energy production in biogas plants offers the possibility of obtaining electricity, heat, and highly efficient manure. The thermal energy generated in an agricultural biogas plant is used to heat the substrate in the fermentation process and can also be used to heat buildings, dry fodder, or wood. Electricity can be used to cover the farm’s needs and sold to an energy company. However, the energy generated in the biogas plant must be of the right quality. One of the main factors describing the quality of electricity is the voltage distortion from the sinusoidal waveform. This paper presents the results of a study of the impact of biogas plant operation on the course of voltage and current in the grid. The theoretical analysis of the voltage distortion mechanism at the point of connection of an agricultural biogas plant was based on a simplified power system model consisting of a voltage source and the equivalent impedance of the power system. According to the theoretical analyses, agricultural biogas plant operation should reduce the voltage distortion factor. In order to confirm this statement, field tests were carried out at three agricultural biogas plants, based on which an analysis was made of the impact of the power generated in the biogas plants on the value of voltage distortion occurring at the point of their connection. However, the field tests did not confirm the conclusion of the theoretical analysis. Only in one case (where the biogas plant was connected near a substation and there was the highest short-circuit power) could it be seen that an increase in generation affected the voltage distortion factor. In the other two cases, generation did not significantly affect the shape of the voltage waveform. However, in each of the biogas plants studied, as the generation power increased, the current distortion factor decreased, suggesting that agricultural biogas plants should operate as close to the rated power as possible.
Comparative Analysis of Combustion Characteristics of a CFB Boiler during the Changes Process between High-Rated Loads and Low-Rated Loads
Authors: Jiang Yu , Tang Zihua , Zhang Xiaoyu , Wang Chao , Song Guoliang , Lyu Qinggang
Published: August 2023

In order to alleviate problems such as large fluctuations in grid load caused by the high proportion of renewable energy, circulating fluidized bed (CFB) power plants undertake the task of rated load regulation. This study discussed the combustion characteristics of a 100 KW CFB boiler during the operation process of varying loads and analyzes the combustion characteristics, load regulation rate and emissions variation law during the operation process of high- and low-rated load intervals. The experimental results showed that under the condition of a high-rated load, the average temperature of each area in the furnace was proportional to the size of the load. Under low-rated load conditions, the temperature change increased first and then decreased with the reduction in load. In the 30% load stage, the lowest temperature in the riser was 740 °C, while the temperature in the loop seal was even as low as 650 °C. The concentrations of O2, CO and unburned carbon mainly depended on the combustion reaction intensity under each load condition, which showed a higher trend at low load (30%). In terms of NOx emission, it was proportional to the load in the high-rated load range. However, the NOx generation at the 30% load was about 30 mg/Nm3(@6%O2) higher than the 50% load. In addition, the regulation load rate (2.5%/min) between high-load conditions exhibited significantly greater than that between low-load conditions (0.78%/min). Therefore, the low-load operation will face problems such as low furnace temperature, uneven gas–solid fluidization, and difficult control of pollutant generation, which need to be paid attention to during operation.
Heartwood Relationship with Stem Diameter in Pinus canariensis Plantations of Gran Canaria, Spain
Authors: Aguilar Luis Fernando Arencibia , Rodríguez Priscila Rodríguez , Biondi Franco
Published: August 2023

The development of stem heartwood and the factors that control it play an important role in tree physiology, thereby impacting demographic and ecological processes of woody species. We investigated the relationships of stem heartwood with site- and tree-level variables in Pinus canariensis plantations. A total of 30 plots were sampled in the island of Gran Canaria, Spain, over a large elevation range (995–1875 m) and on terrain with different slopes (4%–70%) and exposures. The 15 pines closest to each plot center were measured and cored to quantify growth rates and the size of heartwood, also known as “tea”. We used generalized linear mixed models (GLMMs) to account for both fixed and random effects while evaluating the best predictors of heartwood presence. Stem diameter was the variable most correlated with heartwood radius, and allowing for a random slope and intercept of this relationship accounted for spatially related variability. Furthermore, the GLMM model became more effective when the relationship between stem diameter and heartwood was modeled using the presence/absence of the “tea” rather than its measured size. Other site- and tree-level variables either were not statistically significant or improved the model relatively little. Because stem heartwood affects both wood quality and the amount of carbon that trees can store, our findings have implications for forest management and carbon-conscious policies.
Use of a Highly Specialized Biocatalyst to Produce Lactate or Biohydrogen and Butyrate from Agro-Industrial Resources in a Dual-Phase Dark Fermentation
Authors: García-Depraect Octavio , León-Becerril Elizabeth
Published: August 2023

This study aimed at investigating the feasibility of using a highly specialized bacterial inoculum harboring lactic acid bacteria (LAB) and lactate-oxidizing, hydrogen-producing bacteria (LO-HPB) to produce either lactate or biohydrogen and butyrate from several agro-industrial resources via dual-phase dark fermentation. The feedstocks were fruit–vegetable waste, cheese whey, coffee wastewater, tequila vinasse, and maize processing wastewater, and were tested in both mono- and co-fermentation. The results obtained indicated that the biocatalyst used was able to perform a dual-phase lactate fermentation, producing high lactate (13.1–36.4 g/L), biohydrogen (0.2–7.5 NL H2/Lfeedstock, equivalent to 0.3–1.7 mol H2/mol hexose), and butyrate (3.3–13.9 g/L) with all the tested feedstocks. A series of self-fermentation tests were also performed with crude cheese whey and fruit–vegetable waste for comparison purposes. Compared to inoculum-aided fermentations, the self-fermentation exhibited a reduced bioconversion efficiency. Short-length 16S rRNA gene sequencing analysis showed that LO-HPB was the dominant microbial group (86.0%) in the biocatalyst, followed by acetic acid bacteria (5.8%) and LAB (5.7%). As expected, the molecular analysis also showed significant differences in the microbial community structure of the biocatalyst and those that evolved from self-fermentation. Besides lactate fermentation and oxidation, the biocatalyst also assisted the bi-phasic lactate fermentation via oxygen consumption, and apparently, via substrate hydrolysis. Overall, this study can lay the foundation for robust inoculum development, which is of special significance in the field of dark fermentation, and proposes an innovative bioprocess for agro-industrial valorization through a trade-off approach, tailoring the metabolic pathway to the target product(s).
Discrimination of Filter Coffee Extraction Methods of a Medium Roasted Specialty Coffee Based on Volatile Profiles and Sensorial Traits
Authors: Santanatoglia Agnese , Alessandroni Laura , Fioretti Lauro , Sagratini Gianni , Vittori Sauro , Maggi Filippo , Caprioli Giovanni
Published: August 2023

An untargeted gas chromatography-mass spectrometry (GC-MS) approach combined with sensory analysis was used to present the effects of different extraction methods (i.e., Pure Brew, V60, AeroPress, and French Press) on specialty graded Coffea arabica from Kenya. Partial Least Square Discriminant analysis and hierarchical clustering were applied as multivariate statistical tools in data analysis. The results showed good discrimination and a clear clustering of the groups of samples based on their volatile profiles. Similarities were found related to the filter material and shape used for the extraction. Samples extracted with paper filters (V60 and AeroPress) resulted in higher percentages of caramel-, and flowery-related compounds, while from metal filter samples (Pure Brew and French Press), more fruity and roasted coffees were obtained. Discriminant analysis allowed the identification of eight compounds with a high VIP (variable important in projection) discriminant value (i.e., >1), with 2-furanmethanol being the main feature in discrimination. Sensorial analyses were carried out through an expert panel test. The main evaluations revealed the French Press system as the lowest-scored sample in all the evaluated parameters, except for acidity, where its score was similar to V60. In conclusion, the data obtained from GC-MS analyses were in line with the sensorial results, confirming that the extraction process plays a fundamental role in the flavor profile of filter coffee beverages.
Effect of Vacuum Roasting on Total Selenium Content of Selenium-Enriched Rapeseed, Maillard Reaction Products, Oxidative Stability and Physicochemical Properties of Selenium-Enriched Rapeseed Oil
Authors: Xie Qihui , Wang Chengming , Peng Luqiu , Dong Yiyang , Gao Yu , Xu Jing , Ping Hongzheng , Liu Shilin
Published: August 2023

Selenium-enriched rapeseed (SER) is an emerging oil seed. Roasting is beneficial in improving oil yield and promoting the release of micronutrients into SER oil, but high temperatures and dry air lead to selenium loss and fatty acid degradation in SER. To minimize the selenium loss and improve the SER oil quality, this study investigated the effects of vacuum (VC) roasting (90–170 °C for 30 min) on the SER selenium content, Maillard reaction products, oxidative stability, and physicochemical properties of SER oil, with conventional dry air (DA) roasting as the control. The results showed that the selenium loss in VC-roasted SER meals increased from 7.17 to 19.76% (90–170 °C for 30 min), which was 47.13 to 80.48% of that in DA-roasted SER meals, while no selenium was detected in the SER oils. Compared to DA roasting, VC roasting (90–170 °C for 30 min) reduced lipid oxidation products (LOPs), Maillard reaction products (MRPs), and benzo[a]pyrene contents, and increased carotenoids, unsaturated fatty acid contents, reaching a maximum oil yield of 35.58% at a lower temperature (130 °C for 30 min). Selenium contents exhibited a highly significant negative correlation with MRPs and LOPs (p ≤ 0.005). The VC roasting retarded selenium loss and improved SER oil quality compared to conventional DA roasting.
Determination of Metal Content by Inductively Coupled Plasma-Mass Spectrometry in Polish Red and White Wine Samples in Relation to Their Type, Origin, Grape Variety and Health Risk Assessment
Authors: Jakkielska Dorota , Dasteridis Ioannis , Kubicki Maciej , Frankowski Marcin , Zioła-Frankowska Anetta
Published: August 2023

The main objective of the research was to assess the influence of selected factors (type of wine, grape variety, origin, alcohol content and daily consumption) on the concentration levels of 26 elements in 53 Polish wine samples, also using chemometric analysis tools. Concentration of Al, As, B, Ba, Be, Cd, Co, Cr, Cu, Fe, Hg, Li, Mn, Ni, Pb, Sb, Se, Sr, Ti, V, Zn and Zr was analyzed by ICP-MS, while concentration of Ca, Na, K and Mg was determined by ICP-OES. White wines were characterized by higher concentrations of Al, As, Be, Ca, Co, Cu, Fe, Hg, Li, Mg, Na, Pb, Sb, Ti, V, Zn and Zr (mean values: 0.075–86,403 μg·L−1 in white wines, 0.069–81,232 μg·L−1 in red wines). Red wines were characterized by higher concentrations of Ba, Cd, Cr, K, Mn, Se and Sr (mean values: 0.407–1,160,000 μg·L−1 in white wines, 0.448–1,521,363 μg·L−1 in red wines). The results obtained for the health risk assessment indices, including the Target Hazard Quotient (THQ, mean values per glass of wine: 2.097 × 10−5 (Cr)—0.041 (B) in all wines), indicate that the analyzed elements do not show a potential toxic effect resulting from wine consumption. The chemometric analysis confirmed that elements such as Li, Ti, Ca, Mn, Sr, Ba, Zn, Mg, Cu, Se and B were closely related to local conditions and soil properties, and the presence of Fe, Cr, V and Pb was related to contamination of the soil.
Genome-Wide Identification and Expression Profiling Analysis of SWEET Family Genes Involved in Fruit Development in Plum (Prunus salicina Lindl)
Authors: Jiang Cuicui , Zeng Shaomin , Yang Jun , Wang Xiaoan
Published: August 2023

SWEETs (sugars will eventually be exported transporters) play a vital role in longer-distance sugar transportation, and thus control carbon flow and energy metabolism in plants. SWEET genes have been identified in various plant species, but their functions in fruit development remain uncharacterized. Here, we isolated 15 putative PsSWEETs from the Prunus salicina genome. For further analysis, comprehensive bioinformatics methods were applied to determine the gene structure, chromosome distribution, phylogeny, cis-acting regulatory elements, and expression profiles of PsSWEETs. qRT-PCR analysis suggested that these SWEETs might have diverse functions in the development of plum fruit. The relative expression levels of PsSWEET1 and PsSWEET9 were obviously higher in ripened fruit than the ones in other developmental stages, suggesting their possible roles in the transport and accumulation of sugars in plum fruit. Positive correlations were found between the expression level of PsSWEET3/10/13 and the content of sucrose, and the expression level of PsSWEET2 and the content of fructose, respectively, during the development of ‘Furongli’ fruit, suggesting their possible roles in the accumulation of sucrose and fructose. The current study investigated the initial genomic characterization and expression patterns of the SWEET gene family in plum, which could provide a foundation for the further understanding of the functional analysis of the SWEET gene family.
“I’m Afraid If This Goes Wrong… What Will Become of Me?”: The Psychological Experience of Grandparents in Pediatric Palliative Care
Authors: Nogueira Alexandra Jóni , Ribeiro Maria Teresa
Published: August 2023

Portugal has been identified as the European country with the most rapid evolution of Pediatric Palliative Care provision, where approximately 7800 children have life-limiting conditions. This is a highly complex experience not only for the children and their parental caregivers, but also for their healthy siblings and grandparents. The present descriptive-exploratory study seeks to contribute to the understanding of the psychological experience of life-limiting conditions in grandparents. A total of 19 families, consisting of 15 grandmothers and 4 grandfathers, completed a sociodemographic and clinical data sheet and a semi-structured interview was conducted in which they shared their testimony. The results of the thematic analysis highlighted an integrated view on 10 important dimensions in the grandparental experience and promoted creative responses by means of their own perspective. However, it has some limitations, such as the small sample size and the data collection procedure via telephone. The results contribute to the design of specific intervention methodologies in an ecosystemic approach and suggest further research to explore more protective factors and communication with health professionals. For psychological intervention, it is suggested considering the identification of individual and family resources that contribute to the activation of key processes in resilience and posttraumatic growth.
Prevalence and Characteristics of Violence against Paramedics in a Single Canadian Site
Authors: Mausz Justin , Johnston Mandy , Arseneau-Bruneau Dominique , Batt Alan M. , Donnelly Elizabeth A.
Published: August 2023

Violence against paramedics has been described as a ‘serious public health problem’ but one that remains ‘vastly underreported’, owing to an organizational culture that stigmatizes reporting–hindering efforts at risk mitigation in addition to creating a gap in research. Leveraging a novel reporting process developed after extensive stakeholder consultation and embedded within the electronic patient care record, our objective was to provide a descriptive profile of violence against paramedics in a single paramedic service in Ontario, Canada. Between 1 February 2021 and 31 January 2023, a total of 374 paramedics in Peel Region (48% of the workforce) generated 941 violence reports, of which 40% documented physical (n = 364) or sexual (n = 19) assault. The violence was typically perpetrated by patients (78%) and primarily took place at the scene of the 9-1-1 call (47%); however, violent behavior frequently persisted or recurred while in transit to hospital and after arrival. Collectively, mental health, alcohol, or drug use were listed as contributing circumstances in 83% of the violence reports. In all, 81 paramedics were physically harmed because of an assault. On average, our data correspond to a paramedic filing a violence report every 18 h, being physically assaulted every 46 h, and injured every 9 days.
Cervical Spine Instability Screening Tool Thai Version: Assessment of Convergent Validity and Rater Reliability
Authors: Rueangsri Chanyawat , Puntumetakul Rungthip , Leungbootnak Arisa , Sae-Jung Surachai , Chatprem Thiwaphon
Published: August 2023

Neck pain, dizziness, difficulty supporting the head for an extended period, and impaired movement are all symptoms of cervical spine instability, which may produce cervical spondylolisthesis in patients who have more severe symptoms. To avoid problems and consequences, early detection of cervical spine instability is required. A previous study created a Thai-language version of a cervical spine instability screening tool, named the CSI-TH, and evaluated its content validity. However, other characteristics of the CSI-TH still needed to be evaluated. The objective of the current study was to assess the rater reliability and convergent validity of the CSI-TH. A total of 160 participants with nonspecific chronic neck pain were included in the study. The Neck Disability Index Thai version (NDI-TH), the Visual Analog Scale Thai version (VAS-TH), and the Modified STarT Back Screening Tool Thai version (mSBST-TH) were used to evaluate the convergent validity of the CSI-TH. To determine inter- and intra-rater reliabilities, novice and experienced physical therapists were involved. The results showed that rater reliabilities were excellent: the intra-rater reliability was 0.992 (95% CI = 0.989 ± 0.994), and the inter-rater reliability was 0.987 (95% CI = 0.983 ± 0.991). The convergent validities of the VAS-TH, NDI-TH, and mSBST-TH when compared with the CSI-TH were 0.5446, 0.5545, and 0.5136, respectively (p < 0.01). The CSI-TH was developed for use by physical therapists and is reliable. It can be used by physical therapists, whether they are experienced or novices, and has an acceptable correlation to other neck-related questionnaires. The CSI-TH is concise, suitable for clinical use, and lower-priced when compared to the gold standard in diagnosis for patients with cervical spine instability.
Role of STIM1 in the Regulation of Cardiac Energy Substrate Preference
Authors: Liu Panpan , Yang Zhuli , Wang Youjun , Sun Aomin
Published: August 2023

The heart requires a variety of energy substrates to maintain proper contractile function. Glucose and long-chain fatty acids (FA) are the major cardiac metabolic substrates under physiological conditions. Upon stress, a shift of cardiac substrate preference toward either glucose or FA is associated with cardiac diseases. For example, in pressure-overloaded hypertrophic hearts, there is a long-lasting substrate shift toward glucose, while in hearts with diabetic cardiomyopathy, the fuel is switched toward FA. Stromal interaction molecule 1 (STIM1), a well-established calcium (Ca2+) sensor of endoplasmic reticulum (ER) Ca2+ store, is increasingly recognized as a critical player in mediating both cardiac hypertrophy and diabetic cardiomyopathy. However, the cause–effect relationship between STIM1 and glucose/FA metabolism and the possible mechanisms by which STIM1 is involved in these cardiac metabolic diseases are poorly understood. In this review, we first discussed STIM1-dependent signaling in cardiomyocytes and metabolic changes in cardiac hypertrophy and diabetic cardiomyopathy. Second, we provided examples of the involvement of STIM1 in energy metabolism to discuss the emerging role of STIM1 in the regulation of energy substrate preference in metabolic cardiac diseases and speculated the corresponding underlying molecular mechanisms of the crosstalk between STIM1 and cardiac energy substrate preference. Finally, we briefly discussed and presented future perspectives on the possibility of targeting STIM1 to rescue cardiac metabolic diseases. Taken together, STIM1 emerges as a key player in regulating cardiac energy substrate preference, and revealing the underlying molecular mechanisms by which STIM1 mediates cardiac energy metabolism could be helpful to find novel targets to prevent or treat cardiac metabolic diseases.
Antioxidant, Pro-Survival and Pro-Regenerative Effects of Conditioned Medium from Wharton’s Jelly Mesenchymal Stem Cells on Developing Zebrafish Embryos
Authors: Reina Chiara , Cardella Clara , Lo Pinto Margot , Pucci Gaia , Acuto Santina , Maggio Aurelio , Cavalieri Vincenzo
Published: August 2023

Conditioned media harvested from stem cell culturing have the potential to be innovative therapeutic tools against various diseases, due to their high content of growth, trophic and protective factors. The evaluation in vivo of the effects and biosafety of these products is essential, and zebrafish provides an ideal platform for high-throughput toxicological analysis, concurrently allowing the minimization of the use of mammalian models without losing reliability. In this study, we assessed the biological effects elicited by the exposure of zebrafish embryos to a conditioned medium derived from Wharton’s jelly mesenchymal stem cells. By a multiparametric investigation combining molecular, embryological, behavioural and in vivo imaging techniques, we found that exposure to a conditioned medium at a non-toxic/non-lethal dosage triggers antioxidant, anti-apoptotic and pro-regenerative effects, by upregulation of a set of genes involved in antioxidant defence (nrf2, brg1, sirt1, sirt6, foxO3a, sod2 and cat), glycolysis (ldha) and cell survival (bcl2l1, mcl1a and bim), coupled to downregulation of pro-apoptotic markers (baxa, caspase-3a and caspase-8). To our knowledge, this is the first study comprehensively addressing the effects of a conditioned medium on a whole organism from a developmental, molecular and behavioural perspective, and we are fairly confident that it will pave the way for future therapeutic application.
Underfeeding Alters Brain Tissue Synthesis Rate in a Rat Brain Injury Model
Authors: Curl Casey C. , Leija Robert G. , Arevalo Jose A. , Osmond Adam D. , Duong Justin J. , Kaufer Daniela , Horning Michael A. , Brooks George A.
Published: August 2023

Brain injuries (BI) are highly disruptive, often having long lasting effects. Inadequate standard of care (SOC) energy support in the hospital leads to dietary energy deficiencies in BI patients. However, it is unclear how underfeeding (UF) affects protein synthesis post-BI. Therefore, in a rat model, we addressed the issue of UF on the protein fractional synthesis rate (fSR) post-BI. Compared to ad libitum (AL)-fed animals, we found that UF decreased protein synthesis in hind-limb skeletal muscle and cortical mitochondrial and structural proteins (p ≤ 0.05). BI significantly increased protein synthesis in the left and right cortices (p ≤ 0.05), but suppressed protein synthesis in the cerebellum (p ≤ 0.05) as compared to non-injured sham animals. Compared to underfeeding alone, UF in conjunction with BI (UF+BI) caused increased protein synthesis rates in mitochondrial, cytosolic, and whole-tissue proteins of the cortical brain regions. The increased rates of protein synthesis found in the UF+BI group were mitigated by AL feeding, demonstrating that caloric adequacy alleviates the effects of BI on protein dynamics in cortical and cerebellar brain regions. This research provides evidence that underfeeding has a negative impact on brain healing post-BI and that protein reserves in uninjured tissues are mobilized to support cortical tissue repair following BI.
Beyond Strains: Molecular Diversity in Alpha-Synuclein at the Center of Disease Heterogeneity
Authors: Wojewska Marcelina J. , Otero-Jimenez Maria , Guijarro-Nuez Jose , Alegre-Abarrategui Javier
Published: August 2023

Alpha-synucleinopathies (α-synucleinopathies) such as Parkinson’s disease (PD), Parkinson’s disease dementia (PDD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA) are all characterized by aggregates of alpha-synuclein (α-syn), but display heterogeneous clinical and pathological phenotypes. The mechanism underlying this heterogeneity is thought to be due to diversity in the α-syn strains present across the diseases. α-syn obtained from the post-mortem brain of patients who lived with these conditions is heterogenous, and displays a different protease sensitivity, ultrastructure, cytotoxicity, and seeding potential. The primary aim of this review is to summarize previous studies investigating these concepts, which not only reflect the idea of different syn strains being present, but demonstrate that each property explains a small part of a much larger puzzle. Strains of α-syn appear at the center of the correlation between α-syn properties and the disease phenotype, likely influenced by external factors. There are considerable similarities in the properties of disease-specific α-syn strains, but MSA seems to consistently display more aggressive traits. Elucidating the molecular underpinnings of heterogeneity amongst α-synucleinopathies holds promise for future clinical translation, allowing for the development of personalized medicine approaches tackling the root cause of each α-synucleinopathy.
The Stabilization of S100A9 Structure by Calcium Inhibits the Formation of Amyloid Fibrils
Authors: Sanders Ella , Csondor Rebecca , Šulskis Darius , Baronaitė Ieva , Smirnovas Vytautas , Maheswaran Luckshi , Horrocks Jack , Munro Rory , Georgiadou Christina , Horvath Istvan , Morozova-Roche Ludmilla A. , Williamson Philip T. F.
Published: August 2023

The calcium-binding protein S100A9 is recognized as an important component of the brain neuroinflammatory response to the onset and development of neurodegenerative disease. S100A9 is intrinsically amyloidogenic and in vivo co-aggregates with amyloid-β peptide and α-synuclein in Alzheimer’s and Parkinson’s diseases, respectively. It is widely accepted that calcium dyshomeostasis plays an important role in the onset and development of these diseases, and studies have shown that elevated levels of calcium limit the potential for S100A9 to adopt a fibrillar structure. The exact mechanism by which calcium exerts its influence on the aggregation process remains unclear. Here we demonstrate that despite S100A9 exhibiting α-helical secondary structure in the absence of calcium, the protein exhibits significant plasticity with interconversion between different conformational states occurring on the micro- to milli-second timescale. This plasticity allows the population of conformational states that favour the onset of fibril formation. Magic-angle spinning solid-state NMR studies of the resulting S100A9 fibrils reveal that the S100A9 adopts a single structurally well-defined rigid fibrillar core surrounded by a shell of approximately 15–20 mobile residues, a structure that persists even when fibrils are produced in the presence of calcium ions. These studies highlight how the dysregulation of metal ion concentrations can influence the conformational equilibria of this important neuroinflammatory protein to influence the rate and nature of the amyloid deposits formed.
Functions of Plant Phytochrome Signaling Pathways in Adaptation to Diverse Stresses
Authors: Qiu Xue , Sun Guanghua , Liu Fen , Hu Weiming
Published: August 2023

Phytochromes are receptors for red light (R)/far-red light (FR), which are not only involved in regulating the growth and development of plants but also in mediated resistance to various stresses. Studies have revealed that phytochrome signaling pathways play a crucial role in enabling plants to cope with abiotic stresses such as high/low temperatures, drought, high-intensity light, and salinity. Phytochromes and their components in light signaling pathways can also respond to biotic stresses caused by insect pests and microbial pathogens, thereby inducing plant resistance against them. Given that, this paper reviews recent advances in understanding the mechanisms of action of phytochromes in plant resistance to adversity and discusses the importance of modulating the genes involved in phytochrome signaling pathways to coordinate plant growth, development, and stress responses.
Effect of Time on the Properties of Bio-Nanocomposite Films Based on Chitosan with Bio-Based Plasticizer Reinforced with Nanofiber Cellulose
Authors: Janik Weronika , Nowotarski Michał , Ledniowska Kerstin , Biernat Natalia , Abdullah  , Shyntum Divine Yufetar , Krukiewicz Katarzyna , Turczyn Roman , Gołombek Klaudiusz , Dudek Gabriela
Published: August 2023

The deterioration of the performance of polysaccharide-based films over time, particularly their hydrophilicity and mechanical properties, is one of the main problems limiting their applications in the packaging industry. In the present study, we proposed to improve the performance of chitosan-based films through the use of: (1) nanocellulose as an additive to reduce their hydrophilic nature; (2) bio-based plasticizer to improve their mechanical properties; and (3) chestnut extract as an antimicrobial agent. To evaluate their stability over time, the properties of as-formed films (mechanical, hydrophilic, barrier and antibacterial) were studied immediately after preparation and after 7, 14 and 30 days. In addition, the morphological properties of the films were characterized by scanning electron microscopy, their structure by FTIR, their transparency by UV-Vis and their thermal properties by TGA. The films showed a hydrophobic character (contact angle above 100°), barrier properties to oxygen and carbon dioxide and strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria. Moreover, the use of nanofillers did not deteriorate the elongation at breaks or the thermal properties of the films, but their addition reduced the transparency. In addition, the results showed that the greatest change in film properties occurred within the first 7 days after sample preparation, after which the properties were found to stabilize.
Ascites-Derived Organoids to Depict Platinum Resistance in Gynaecological Serous Carcinomas
Authors: Arias-Diaz Andrea Estrella , Ferreiro-Pantin Miriam , Barbazan Jorge , Perez-Beliz Edurne , Ruiz-Bañobre Juan , Casas-Arozamena Carlos , Muinelo-Romay Laura , Lopez-Lopez Rafael , Vilar Ana , Curiel Teresa , Abal Miguel
Published: August 2023

Gynaecological serous carcinomas (GSCs) constitute a distinctive entity among female tumours characterised by a very poor prognosis. In addition to late-stage diagnosis and a high rate of recurrent disease associated with massive peritoneal carcinomatosis, the systematic acquisition of resistance to first-line chemotherapy based on platinum determines the unfavourable outcome of GSC patients. To explore the molecular mechanisms associated with platinum resistance, we generated patient-derived organoids (PDOs) from liquid biopsies of GSC patients. PDOs are emerging as a relevant preclinical model system to assist in clinical decision making, mainly from tumoural tissue and particularly for personalised therapeutic options. To approach platinum resistance in a GSC context, proficient PDOs were generated from the ascitic fluid of ovarian, primary peritoneal and uterine serous carcinoma patients in platinum-sensitive and platinum-resistant clinical settings from the uterine aspirate of a uterine serous carcinoma patient, and we also induced platinum resistance in vitro in a representative platinum-sensitive PDO. Histological and immunofluorescent characterisation of these ascites-derived organoids showed resemblance to the corresponding original tumours, and assessment of platinum sensitivity in these preclinical models replicated the clinical setting of the corresponding GSC patients. Differential gene expression profiling of a panel of 770 genes representing major canonical cancer pathways, comparing platinum-sensitive and platinum-resistant PDOs, revealed cellular response to DNA damage stimulus as the principal biological process associated with the acquisition of resistance to the first-line therapy for GSC. Additionally, candidate genes involved in regulation of cell adhesion, cell cycles, and transcription emerged from this proof-of-concept study. In conclusion, we describe the generation of PDOs from liquid biopsies in the context of gynaecological serous carcinomas to explore the molecular determinants of platinum resistance.
Integrating Single-Cell RNA-Seq and Bulk RNA-Seq Data to Explore the Key Role of Fatty Acid Metabolism in Breast Cancer
Authors: Chen Yongxing , Wu Wei , Jin Chenxin , Cui Jiaxue , Diao Yizhuo , Wang Ruiqi , Xu Rongxuan , Yao Zhihan , Li Xiaofeng
Published: August 2023

Cancer immune escape is associated with the metabolic reprogramming of the various infiltrating cells in the tumor microenvironment (TME), and combining metabolic targets with immunotherapy shows great promise for improving clinical outcomes. Among all metabolic processes, lipid metabolism, especially fatty acid metabolism (FAM), plays a major role in cancer cell survival, migration, and proliferation. However, the mechanisms and functions of FAM in the tumor immune microenvironment remain poorly understood. We screened 309 fatty acid metabolism-related genes (FMGs) for differential expression, identifying 121 differentially expressed genes. Univariate Cox regression models in The Cancer Genome Atlas (TCGA) database were then utilized to identify the 15 FMGs associated with overall survival. We systematically evaluated the correlation between FMGs’ modification patterns and the TME, prognosis, and immunotherapy. The FMGsScore was constructed to quantify the FMG modification patterns using principal component analysis. Three clusters based on FMGs were demonstrated in breast cancer, with three patterns of distinct immune cell infiltration and biological behavior. An FMGsScore signature was constructed to reveal that patients with a low FMGsScore had higher immune checkpoint expression, higher immune checkpoint inhibitor (ICI) scores, increased immune microenvironment infiltration, better survival advantage, and were more sensitive to immunotherapy than those with a high FMGsScore. Finally, the expression and function of the signature key gene NDUFAB1 were examined by in vitro experiments. This study significantly demonstrates the substantial impact of FMGs on the immune microenvironment of breast cancer, and that FMGsScores can be used to guide the prediction of immunotherapy efficacy in breast cancer patients. In vitro experiments, knockdown of the NDUFAB1 gene resulted in reduced proliferation and migration of MCF-7 and MDA-MB-231 cell lines.