bluuebunny/crossref_metadata_2025 huggingface dataset:
Interactions between arbuscular mycorrhizal fungi and phosphate-soluble bacteria affect ginsenoside compositions by modulating the C:N:P stoichiometry in Panax ginseng
Authors: Mu Peng , Ding Guanzhong , Zhang Yue , Jin Qiao , Liu Zhengbo , Guan Yiming , Zhang Linlin , Liang Chijia , Zhou Fan , Liu Ning
Published: October 2024

The biomass production as well as the accumulation of secondary metabolites of plant is highly determined by the absorption of nutritional elements, in particular nitrogen (N) and phosphorus (P). Arbuscular mycorrhizal fungi (AMF) can absorb soluble P and transport it to plants, while phosphate solubilizing bacteria (PSB) can increase the content of solubilizing P in soil. Previous studies have identified the effects of either AMF or PSB inoculation on altering plant C:N:P stoichiometry, whether AMF interact with PSB in promoting plant growth and changing elemental concentration and composition of secondary metabolites by altering plant C:N:P stoichiometry remains ambiguous. In this study, we investigated the effects of inoculation of AMF, PSB, and their co-inoculation AMP (AMF and PSB) on the biomass growth, the C:N:P stoichiometry, the core microorganisms of rhizosphere soil, and the ginsenoside compositions of ginseng (Panax ginseng). The results showed that compared to control or single inoculation of AMF or PSB, co-inoculation of AMF and PSB significantly increased the AMF colonization rate on ginseng roots, increased the biomass of both above and under-ground parts of ginseng. Similarly, co-inoculation of AMF and PSB substantially increased the concentrations of N and P, reduced the ratios of C:P and N:P in the above-ground part of ginseng. The co-inoculation of AMF and PSB also increased concentrations of total ginsenosides and altered the compositions of ginsenosides in both the above and under-ground parts of ginseng. Analysis the rhizosphere microorganism showed that the co-inoculation of AMF and PSB recruited distinct core microorganisms that differ from the control and treatments with single inoculation of AMF or PSB. Our results suggested that PSB inoculation enhanced the positive effect of AMF in improving the absorption of nutrimental elements, altered the C:N:P stoichiometry and, ginsenosides concentration and composition of ginseng, influenced the plant rhizosphere microbial community. These findings offer valuable insights into enhancing plant biomass production and promoting secondary metabolites by improving the plant-fungi-bacterial relationships.
Clonal and resistance profiles of fluoroquinolone-resistant uropathogenic Escherichia coli in countries with different practices of antibiotic prescription
Authors: Choudhury Debarati , Alanbari Rawan , Saveliev Pauline , Sokurenko Evgeni , Fuzi Miklos , Tchesnokova Veronika
Published: October 2024

Background

Antibiotic prescription practices differ between countries, influencing regional antimicrobial resistance prevalence. However, comparisons of clonal diversity among resistant bacteria in countries with different prescribing practices are rare. The rise of fluoroquinolone-resistant Escherichia coli (FQREC), often multidrug-resistant, exacerbates global antibiotic resistance. Unlike in the USA, antibiotics are commonly dispensed in Iraq without prescriptions, leading to widespread overuse and misuse. This study aimed to assess the impact of varying antibiotic use practices on FQREC diversity.

Methods

We compared FQREC prevalence, multidrug resistance, and clonality of FQREC among E. coli isolated from urine submitted between 2017 and 2018 to three US hospitals and two Iraqi hospitals. All FQREC isolates were analyzed for QRDR mutations and the presence of PMQR genes. A subset of FQREC strains from the ST131-H30R/Rx subgroups underwent whole-genome sequencing (WGS) and phylogenetic analysis.

Results

E. coli from Iraq showed significantly higher resistance to all tested antibiotics compared to those from the USA, with 76.2% being FQREC versus 31.2% in the USA (p < 0.01). Iraqi FQREC strains were more frequently multidrug resistant. The predominant subgroup in both countries was ST131-H30, with the notable absence of ST1193 among Iraqi FQREC. Iraqi-origin ST131-H30 strains exhibited higher minimum inhibitory concentrations (MICs) for ciprofloxacin and greater resistance to third-generation cephalosporins (3GC), trimethoprim/sulfamethoxazole (TMP/STX), and imipenem (IMI) than those from the USA. Increased 3GC resistance in Iraqi strains was linked to a higher proportion of blaCTX-M-15-carrying H30Rx subclade isolates. Additionally, Iraqi H30 strains exhibited higher MICs for fluoroquinolones due to more frequent carriage of PMQR determinants compared to US strains. Whole-genome sequencing was performed on 46 Iraqi and 63 US H30 isolates. Phylogenetic analysis revealed two clades—H30R and H30Rx—present in both countries, with isolates from both regions distributed throughout, without the emergence of distinct new major subclones. However, Iraqi isolates tended to cluster in separate subclades, indicating endemic circulation of the strain groups.

Conclusion

In regions like Iraq, where antibiotics are overused and misused, resistance among uropathogenic E. coli to various antibiotics is significantly higher. Most Iraqi resistant strains belong to well-known international groups, and no new highly successful strains have emerged. The absence of ST1193 in Iraq may reflect regional, socioeconomic, demographic, or cultural factors that hinder the success of certain strain groups in the country.
Omicron XBB.1.5 subvariant causes severe pulmonary disease in K18-hACE-2 mice
Authors: Elsharkawy Amany , Stone Shannon , Guglani Anchala , Patterson Lila D. , Ge Chunyu , Dim Chinonye , Miano Joseph M. , Kumar Mukesh
Published: October 2024

Owing to their continuous evolution, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) display disparate pathogenicity in mouse models. Omicron and its sublineages have been dominant worldwide. Compared to pre-Omicron VOCs, early Omicron subvariants reportedly cause attenuated disease in human ACE-2-expressing mice (K18-hACE-2). In late 2022, the frequency of Omicron subvariant XBB.1.5 rapidly increased and it progressively replaced other circulating strains. The emergence of new strains requires current SARS-CoV-2 clinical animal model re-evaluation. In this study, we aim to characterize XBB.1.5 pathogenesis in K18-hACE-2. Herein, we demonstrated that XBB.1.5 infection is associated with significant weight loss, severe lung pathology, and substantial mortality. Intranasal XBB.1.5 infection resulted in 100% mortality in K18-hACE2 mice. High virus titers were detected in the lungs on days 3 and 5 after infection. Moreover, XBB.1.5 productively infected the cells within the nasal turbinate, olfactory bulb, intestines, and kidneys. In addition, in a subset of infected mice, we detected high virus titers in the brain. Consistently, we detected high viral antigen expression in the lungs. Furthermore, we observed severe lung injury hallmarks (e.g., immune cell infiltration, perivascular cuffing, and alveolar consolidation). Using immunofluorescence labeling and cytometric analysis, we revealed that XBB.1.5 infection leads to CD45+ cell influx into the lung parenchyma. We further demonstrated that most immune infiltrates are CD11b+ CD11c+ dendritic cells. Additionally, we detected significant induction of proinflammatory cytokines and chemokines in infected lungs. Taken together, our data show that Omicron subvariant XBB.1.5 is highly pathogenic in K18-hACE2 mice.
Branched oncolytic peptides target HSPGs, inhibit metastasis, and trigger the release of molecular determinants of immunogenic cell death in pancreatic cancer
Authors: Rencinai Alessandro , Tollapi Eva , Marianantoni Giulia , Brunetti Jlenia , Henriquez Tania , Pini Alessandro , Bracci Luisa , Falciani Chiara
Published: October 2024

Immunogenic cell death (ICD) can be exploited to treat non-immunoreactive tumors that do not respond to current standard and innovative therapies. Not all chemotherapeutics trigger ICD, among those that do exert this effect, there are anthracyclines, irinotecan, some platinum derivatives and oncolytic peptides. We studied two new branched oncolytic peptides, BOP7 and BOP9 that proved to elicit the release of damage-associated molecular patterns DAMPS, mediators of ICD, in pancreatic cancer cells. The two BOPs selectively bound and killed tumor cells, particularly PANC-1 and Mia PaCa-2, but not cells of non-tumor origin such as RAW 264.7, CHO-K1 and pgsA-745. The cancer selectivity of the two BOPs may be attributed to their repeated cationic sequences, which enable multivalent binding to heparan sulfate glycosaminoglycans (HSPGs), bearing multiple anionic sulfation patterns on cancer cells. This interaction of BOPs with HSPGs not only fosters an anti-metastatic effect in vitro, as demonstrated by reduced adhesion and migration of PANC-1 cancer cells, but also shows promising tumor-specific cytotoxicity and low hemolytic activity. Remarkably, the cytotoxicity induced by BOPs triggers the release of DAMPs, particularly HMGB1, IFN-β and ATP, by dying cells, persisting longer than the cytotoxicity of conventional chemotherapeutic agents such as irinotecan and daunorubicin. An in vivo assay in nude mice showed an encouraging 20% inhibition of tumor grafting and growth in a pancreatic cancer model by BOP9.
Color-induced cognitive conflicts affect muscle activity prior to gait initiation in the Go/No-go task
Authors: Horinouchi Takayuki , Ishida Haruki , Yang Kangjing , Li Jingnan , Morishita Takuya , Watanabe Tatsunori , Kirimoto Hikari
Published: October 2024

Introduction

In traffic rule, green/blue means go, and red means stop. It has been shown that this prior knowledge about traffic signal colors can affect reaction times (RTs). For example, RTs are longer when responding to a red “Go” signal and withholding the response to a blue “No-go” signal (Red Go/Blue No-go task) than when responding to a blue “Go” signal and withholding the response to a red “No-go” signal (Blue Go/Red No-go task), when responses are provided by button press. However, it remains unknown whether this holds in different actions. The aim of this study was to investigate the effect of prior knowledge of color on gait initiation in a Go/No-go task.

Methods

Seventeen participants performed Green Go/Red No-go and Red Go/Green No-go tasks, in which they stepped forward from a force plate in response to a green or red signal and withhold the response to red or green signal, respectively. We recorded the center of pressure (COP) and electromyogram (EMG) from the bilateral tibialis anterior muscles during gait initiation.

Results

The onset of COP movement and toe-off time as well as COP displacements did not differ between the Go/No-go tasks. The EMG onset for the stance leg was delayed in the Red Go/Green No-go than Green Go/Red No-go task.

Discussion

These findings suggest that the conflict between prior knowledge of color related to traffic rule and the meaning of the stimulus color affects muscle activity but not COP characteristics during gait initiation, highlighting two distinct motor control mechanisms, where the initial phase is influenced by cognitive load while the subsequent phase remains unaffected. This dissociation suggests that the later phase of gait initiation relies on robust spinal loops and central pattern generators, which are less influenced by cognitive factors such as prior knowledge.
Functional Near-Infrared Spectroscopy in neurodegenerative disease: a review
Authors: Xie Lei , Liu Yong , Gao Yuling , Zhou Jiaqi
Published: October 2024

In recent years, with the aggravation of aging, the incidence of neurodegenerative diseases is increasing year by year, and the prognosis of patients is poor. Functional Near-Infrared Spectroscopy (fNIRS) is a new and non-invasive neuroimaging technology, which has been gradually deepened in the application research of neurodegenerative diseases by virtue of its unique neurooxygen signal brain functional imaging characteristics in monitoring the disease condition, making treatment plans and evaluating the treatment effect. In this paper, the mechanism of action and technical characteristics of fNIRS are briefly introduced, and the application research of fNIRS in different neurodegenerative diseases is summarized in order to provide new ideas for future related research and clinical application.
Effect of fermentation, malting and ultrasonication on sorghum, mopane worm and Moringa oleifera: improvement in their nutritional, techno-functional and health promoting properties
Authors: Maleke Mpho Sebabiki , Adebo Oluwafemi Ayodeji , Wilkin Jonathan , Ledbetter Moira , Feng Xi , Gieng John , Molelekoa Tumisi Beiri Jeremiah
Published: October 2024

Background

Food processing offers various benefits that contribute to food nutrition, food security and convenience. This study investigated the effect of three different processes (fermentation, malting and ultrasonication) on the nutritional, techno-functional and health-promoting properties of sorghum, mopane worm and Moringa oleifera.

Methods

The fermented and malted flours were prepared at 35°C for 48 h, and for ultrasonication, samples were subjected to 10 min at 4°C with amplitudes of 40–70 Hz. The biochemical, nutritional quality and techno-functional properties of the obtained flours were analysed using standard procedures.

Results

Fermentation resulted in significantly lower pH and higher titratable acidity in sorghum and mopane worm (4.32 and 4.76; 0.24 and 0.69% lactic acid, respectively), and malting resulted in higher total phenolic content and total flavonoid content in sorghum (3.23 mg GAE/g and 3.05 mg QE/g). Ultrasonication resulted in higher protein and fibre in raw sorghum flour (13.38 and 4.53%) and mopane worm (56.24 and 11.74%) while raw moringa had the highest protein (30.68%). Biomodification by fermentation in sorghum led to higher water and oil holding capacity and increased dispersibility in the ultrasonicated samples. Ultrasonication of mopane worms led to higher water holding capacity, oil holding capacity and dispersibility. Lightness was found to be significantly higher in the fermented samples in sorghum and mopane worm. Raw moringa had the greatest lightness compared to the ultrasonicated moringa. Moringa had the most redness and browning index among all samples.

Conclusion

In this study, all the investigated processes were found to have caused variations in flours’ biochemical, nutritional and techno-functional properties. Ultrasonication process was noteworthy to be the most efficient to preserve the nutritional value in sorghum, mopane worm and M. oleifera flours.
Systemic metastasis in malignant solitary fibrous tumor of the liver: two case reports and literature review
Authors: Wei Pengcheng , Lo Chen , Gao Jie , Zhu Jiye , Sun Xin , Li Zhao
Published: October 2024

Solitary fibrous tumor of the liver (SFTL) is an exceptionally rare mesenchymal tumor, with only 117 cases reported in the literature. While most SFTs are benign, some exhibit malignant behavior, including local recurrence and metastasis. This report presents two cases of SFTL with systemic metastases, both involving prior intracranial tumors. The first case, a 52-year-old woman, discovered a liver mass incidentally during a routine physical exam. Subsequent investigations revealed potential bone metastasis, and biopsy confirmed SFT. She received two TACE procedures, anlotinib targeted therapy, and radiotherapy for the iliac bone lesion, resulting in stable disease with reduction in lesion size. The second case, a 46-year-old man, presented with multiple liver, pelvic, and lung lesions following pelvic tumor resection, with pathology confirming SFT. He was treated with long-term anlotinib therapy, CyberKnife for hepatic, lung, and pelvic lesions, and radiofrequency ablation for hepatic lesions. Postoperative recovery was uneventful, with no tumor progression on follow-up. SFTL presents with atypical clinical and imaging features, and diagnosis requires pathological and genetic confirmation. Radical resection is preferred for solitary tumors, while comprehensive treatment, including surgery and long-term follow-up, is essential for cases with recurrence or metastasis.
Analysis of risk factors for intraoperative bleeding in patients with Siewert type II esophagogastric junction adenocarcinoma treated by two minimally invasive surgeries and its influence on prognosis: a retrospective study
Authors: Lan Yang , Shen Jian , Liu Ruqian , Jiang Kai , Qiu Mingyuan , Wang Shuai , Lin Zhou
Published: October 2024

Background

The present study aimed to analyze the independent risk factors for intraoperative bleeding in Siewert II adenocarcinoma of the esophagogastric junction (AEG) using two minimally invasive surgical approaches, namely, the laparoscopy-assisted abdominal trans-hiatal (LTH) method and transthoracic-laparoscopic esophagectomy (TLE).

Methods

The clinical data of 100 patients with SiewertII AEG admitted to our hospital from October 2017 to October 2020 were retrospectively analyzed. According to the type of surgery, the patients were divided into LTH approach group and TLE approach group. The differences between the clinical characteristics of the patients in different groups and the differences in the intraoperative bleeding and prognosis between different surgical procedures were analyzed and compared using the t-test and chi-squared test. Multiple linear regression was used to identify the independent risk factors affecting the amount of intraoperative bleeding in patients.

Results

The results of this study showed that patients in the LTH group had significantly less intraoperative bleeding and operative time and significantly better postoperative recovery than the TLE group. The results of multivariate linear regression showed that the combined trans-thoracic-abdominal approach (P=0.000), advanced age (P=0.014), larger BMI (P=0.000), and larger tumor diameter (P=0.001) were the independent risk factors influencing the increase in intraoperative bleeding.

Conclusion

In addition to the conventional factors that affect intraoperative bleeding, such as the patient’s general condition, operation time, and tumor size, LTH surgery is another way to avoid intraoperative bleeding for Siewert type II AEG patients and can significantly improve postoperative recovery.
Delivery room resuscitation and short-term outcomes in very preterm infants: a multicenter cross-sectional study in China
Authors: Lin Hanni , Yu Zhangbin , Huang Jinjie , Yang Ting , Duan Shitao , Guo Yanping , Zeng Shujuan , Jiang Ping , Wang Rui , Zhang Jing , Ding Lu , Liu Jiebo
Published: October 2024

Objective

To explore the risk factors of delivery room (DR) resuscitation and assess the association of DR resuscitation with neonatal outcomes in very preterm infants (VPIs).

Methods

A multicenter retrospective cross-sectional study included VPIs with gestational age (GA) <32 weeks born between January, 2022 and June, 2023 and admitted to neonatal intensive care units of six tertiary hospitals in Shenzhen within 24 h after birth. They were divided into routine care group, positive-pressure ventilation (PPV) group, and endotracheal intubation (ETT) group based on the highest intensity of resuscitation received at birth. The association of antepartum and intrapartum risk factors and short-term outcomes with the intensity of DR resuscitation was evaluated.

Results

Of 683 infants included in this study, 170 (24.9%) received routine care, 260 (38.1%) received bag and mask ventilation or T-piece ventilation and 253 (37%) received ETT. Among the antepartum and intrapartum factors, exposure to antenatal steroids (ANS) decreased the likelihood of ETT. Increasing GA decreased the likelihood of receiving a higher level of DR resuscitation. Among the neonatal outcomes, increasing intensity of DR resuscitation was associated with a raise in the risk of Bronchopulmonary dysplasia. Higher levels of DR resuscitation were associated with the risk of early-onset sepsis. ETT was significantly associated with an increased risk of death.

Conclusion

Among VPIs, low GA and no ANS use increased the risk of high-intensity DR resuscitation interventions; and those who receiving ETT were associated with an increased risk of adverse clinical outcomes.
Autophagy is a promising process for linking inflammation and redox homeostasis in Down syndrome
Authors: Ma Xuehai , Li Weimin , Ma Jun , Han Zhongcheng , Deng Shoulong , Wang Sutian
Published: October 2024

Trisomy 21, characterized by the presence of an additional chromosome 21, leads to a set of clinical features commonly referred to as Down syndrome (DS). The pathological phenotypes observed in DS are caused by a combination of factors, such as mitochondrial dysfunction, neuroinflammation, oxidative stress, disrupted metabolic patterns, and changes in protein homeostasis and signal transduction, and these factors collectively induce neurological alterations. In DS, the triplication of chromosome 21 and the micronuclei arising from the missegregation of chromosomes are closely associated with inflammation and the development of redox imbalance. Autophagy, an essential biological process that affects cellular homeostasis, is a powerful tool to facilitate the degradation of redundant or dysfunctional cytoplasmic components, thereby enabling the recycling of their constituents. Targeting the autophagy process has been suggested as a promising method to balance intracellular inflammation and oxidative stress and improve mitochondrial dysfunction. In this review, we summarize the role of autophagy in regulating inflammation and redox homeostasis in DS and discuss their crosslinks. A comprehensive elucidation of the roles of autophagy in DS offers novel insights for the development of therapeutic strategies aimed at aneuploidy-associated diseases.
Analysis of differences in fossil fuel consumption in the world based on the fractal time series and complex network
Authors: Zhang Lin , Jian Xiao , Ma Yuxuan
Published: October 2024

Fossil fuels remain indispensable energy resources despite their non-renewable nature. Understanding the patterns of global fossil fuel consumption is essential for energy security and policy-making. This study employs complex network theory and fractal time series analysis to explore the underlying dynamics and patterns of fossil fuel consumption globally, with a focus on coal, oil, and gas consumption.The study applies the Hurst index to raw fossil fuel consumption data to identify fractal characteristics. Additionally, the visibility graph method is used to convert time series data into complex networks, allowing further analysis of consumption patterns. The study examines fossil fuel consumption in 38 countries to assess global trends and differences. The analysis reveals that global fossil fuel consumption follows a fractal time series pattern, with Hurst index values exceeding 0.9, indicating long-term memory characteristics. The application of the visibility graph method demonstrates variations in the Hurst index of degree distribution, enabling the differentiation of consumption patterns across regions. The method also uncovers distinct features of coal, oil, and gas consumption when viewed from a network perspective. The findings suggest that fossil fuel consumption has predictable long-term patterns, which are crucial for assessing future energy demands. The study highlights the importance of legislative measures to safeguard fossil fuel resources, especially for countries like China, where energy security and international competitiveness are paramount. Understanding these consumption patterns could guide future energy policies aimed at managing non-renewable resources more effectively.
A scoping review of preclinical intensive care unit-acquired weakness models
Authors: Yu Qingmei , Song Jiamei , Yang Luying , Miao Yanmei , Xie Leiyu , Ma Xinglong , Xie Peng , Chen Shaolin
Published: October 2024

Background

Animal models focusing on neuromuscular outcomes are crucial for understanding the mechanisms of intensive care unit-acquired weakness (ICU-AW) and exploring potential innovative prevention and treatment strategies.

Aim

To analyse and evaluate preclinical ICU-AW models.

Methods

We manually searched five English and four Chinese databases from 1 January 2002, to 1 February 2024, and reviewed related study references. Full-text publications describing animal models of muscle weakness and atrophy in critical illness were included. Detailed information about model types, animal species, sex, age, induction methods, outcome measures, drawbacks and strengths was extracted from each included study.

Results

A total of 3,451 citations were initially retrieved, with 84 studies included in the final analysis. The most frequently studied animal model included rodents (86.9%), 64.3% of which were male animals. ICU-AW animal models were mostly induced by comprehensive intensive care unit (ICU) interventions (38.1%) and sepsis (51.2%). Most studies focused on limb muscles (66.7%), diaphragm muscles (21.4%) or both (9.5%). Reported outcomes primarily included muscular pathological changes (83.3%), electrophysiological examinations of muscles (57.1%) and animal grip strength (16.6%). However, details such as animal age, mortality data, experimental design, randomisation, blinding, sample size and interventions for the experimental group and/or control group were inadequately reported.

Conclusion

Many preclinical models are used to study ICU-AW, but the reporting of methodological details is often incomplete. Although current ICU animal models can mimic the characteristics of human ICU-AW, there is no standard model. Future preclinical studies should develop a standard ICU-AW animal model to enhance reproducibility and improve scientific rigor in exploring the mechanisms and potential treatment of ICU-AW.
Comparative analysis of transcriptome in oil biosynthesis between seeds and non-seed tissues of Symplocos paniculata fruit
Authors: Liu Qiang , Chen Yunzhu , Chen Jingzhen , Li Peiwang , Jiang Lijuan , Li Changzhu , Zeng Wenbin , Yang Yan
Published: October 2024

The Symplocos paniculata, a woody oil plant, has garnered attention for its oil-rich fruit, which exhibits potential for both oil production and ecological restoration endeavors, thereby presenting substantial developmental value. However, the comprehension of the distinctive oil biosynthesis and deposition strategies within the fruit’s various compartments, coupled with the tissue-specific biosynthetic pathways yielding optimal fatty acid profiles, remains in its infancy. This investigation was designed to delineate the tissue specificity of oil biosynthetic disparities and to elucidate the molecular underpinnings within the fruit mesocarp and seeds of S. paniculata, employing lipidomic and transcriptomic analyses. The results revealed that oil biosynthesis within the fruit mesocarp commences approximately 40 days prior to that within the seeds, with a concomitant higher lipid content observed in the mesocarp, reaching 43% as opposed to 30% in the seeds. The fruit mesocarp was found to be enriched with palmitic acid (C16:0) and exhibited a harmonious ratio of saturated, monounsaturated, to polyunsaturated fatty acids (SFA: MUFA: PUFA=1:1:1), in stark contrast to the seed oil, which is predominantly composed of unsaturated fatty acids, accounting for 90% of its total FA content. Microstructural assessments have unveiled divergent oil deposition modalities; the fruit mesocarp oils are predominantly sequestered within oil cells (OC) and a spectrum of lipid droplets (LD), whereas the seeds predominantly harbor uniformly-sized LD. The expression patterns of pivotal genes implicated in oil biosynthesis were observed to be markedly contingent upon the tissue type and developmental stage. Notably, the light-responsive fatty acid synthase (FAS) gene demonstrated preferential transcription within the fruit mesocarp. In contrast, genes pivotal for carbon chain elongation, such as 3-ketoacyl-ACP synthase II (KASII) and fatty acyl-ACP thioesterase A (FATA), and desaturation, typified by Stearoyl-ACP desaturase (SAD) and Fatty Acid Desaturase (FAD), were noted to be more robustly transcribed within the seeds. Furthermore, isoenzyme gene families integral to the assembly of triacylglycerol (TAG), including long-chain acyl-CoA synthetases (LACSs), glycerol-3-phosphate acyltransferases (GPATs), and lysophosphatidic acid acyltransferases (LPATs), exhibited pronounced tissue specificity. This research endeavors to clarify the molecular regulatory mechanisms that oversee oil biosynthesis within both seed and non-seed tissues of oilseed-bearing plants with entire fruits. Collectively, these findings lay the groundwork and offer technical scaffolding for future targeted cultivation of woody oil plants, with the ultimate aim of augmenting fruit oil yield and refining FA compositions.
Trait-customized sampling of core collections from a winter wheat genebank collection supports association studies
Authors: Berkner Marcel O. , Jiang Yong , Reif Jochen C. , Schulthess Albert W.
Published: October 2024

Subsampling a reduced number of accessions from ex situ genebank collections, known as core collections, is a widely applied method for the investigation of stored genetic diversity and for an exploitation by breeding and research. Optimizing core collections for genome-wide association studies could potentially maximize opportunities to discover relevant and rare variation. In the present study, eight strategies to sample core collections were implemented separately for two traits, namely susceptibility to yellow rust and stem lodging, on about 6,300 accessions of winter wheat (Triticum aestivum L.). Each strategy maximized different parameters or emphasized another aspect of the collection; the strategies relied on genomic data, phenotypic data or a combination thereof. The resulting trait-customized core collections of eight different sizes, covering the range between 100 and 800 accession samples, were analyzed based on characteristics such as population stratification, number of duplicate genotypes and genetic diversity. Furthermore, the statistical power for an association study was investigated as a key criterion for comparisons. While sampling extreme phenotypes boosts the power especially for smaller core collections of up to 500 accession samples, maximization of genetic diversity within the core collection minimizes population stratification and avoids the accumulation of less informative duplicate genotypes when increasing the size of a core collection. Advantages and limitations of different strategies to create trait-customized core collections are discussed for different scenarios of the availability of resources and data.
Promoting salutogenic pathways to health through complementary and integrative health approaches
Authors: Quinlan Erin Burke , Baumgartner Jennifer , Chen Wen G. , Weber Wendy , Horgusluoglu Emrin , Edwards Emmeline
Published: October 2024

Health restoration and disease prevention are important strategies to achieve health and well-being. This Perspective provides a conceptual overview of the key concepts of salutogenesis (health restoration), chronic stress, resilience, and emotional well-being, and describes how they are distinct and interrelated. We posit, and demonstrate through scientific evidence, that complementary and integrative health approaches, including mind and body interventions, can be used to mitigate the effects of chronic stress and promote salutogenic pathways. Our goal is to identify research gaps and opportunities and suggest ways to advance the knowledge base for mechanistic and clinical research in this field.
Mixing transcutaneous vagal nerve stimulation and galvanic cutaneous stimulation to decrease simulator adaptation syndrome
Authors: Gálvez-García Germán , Mena-Chamorro Patricio , Espinoza-Palavicino Tomás , Romero-Arias Tatiana , Barramuño-Medina Mauricio , Bascour-Sandoval Claudio
Published: October 2024

Purpose

Simulator Adaptation Syndrome arises from a perceptual discordance between expected and actual motion, giving rise to symptoms such as nausea and disorientation. This research focused on determining the benefit of Transcutaneous Vagal Nerve Stimulation (tVNS) and Galvanic Cutaneous Stimulation (GCS), where both were applied in conjunction, as compared to their administration in isolation, to decrease Simulator Adaptation Syndrome (SAS).

Method

A driving simulation study was proposed where SAS, body balance, and driving performance were measured. These measurements were taken during seven different stimulation scenarios with a baseline condition without stimulation compared against tVNS and GCS conditions.

Results

The main result showed that the combination of tVNS and GCS reduced SAS and improved body balance and driving performance more successfully than their administration in isolation.

Conclusion

Similar neuromodulation in the temporoparietal junction is proposed to mitigate SAS for GCS and tVNS (although additional explanations are discussed). Applying both techniques simultaneously is encouraged to decrease SAS in future interventions.
Art therapy and neuroscience: evidence, limits, and myths
Authors: Strang Christianne E.
Published: October 2024

The evidence base for the effectiveness of art therapy continues to grow, even as a mechanistic understanding of how art therapy works remains limited. One promising avenue for increasing our understanding of how and why art therapy works is through the lens of neuroscience. A neuroscience-based approach to art therapy provides opportunities for improving understanding of the neural processes that underlie the complex interaction between perception, cognition, emotion and behavior that play out in the art therapy process. Understanding how therapeutic change occurs can result in improved treatment and better outcomes for clients. However, it can be tricky to connect art therapy and psychological theory directly to neural responses. The purposes of this perspective are to provide an overview of the current evidence and limits of neurobiological concepts of neuroplasticity, mirror systems, and interoception as applied to art therapy practice, and to provide updated information about outdated concepts that are still actively used in clinical practice. Critical analysis and understanding of the current scientific knowledge base can then be used to guide art therapy practice and support the development of hypothesis-based research to determine the primary mechanisms that drive the observed effects of art therapy interventions.
Unveiling the grip of mobile phone addiction: an in-depth review
Authors: Li Jinyu , Yang Hong
Published: October 2024

Mobile Phone Addiction represents an emergent addictive disorder that gravely jeopardizes the physical and mental health of adolescents worldwide, necessitating exhaustive research. Current reviews of MPA are in dire need of updates and enhancements. Therefore, this review aggregates the extant research spanning the past two decades on the prevalence, pathogenesis, comorbidities, assessment, and treatment of MPA, aiming to furnish a reference for future investigations into this condition.
Connections between eating psychopathology, loneliness, and quality of life: insights from a multi-center study
Authors: Todisco Patrizia , Maragno Laura , Marzotto Anna , Mezzani Barbara , Conti Fabio , Maggi Luca , Meneguzzo Paolo
Published: October 2024

Background

Eating disorders (ED) involve dysfunctional attitudes towards food intake, affecting physical and psychosocial well-being. These disorders significantly impact various domains of life and can lead to a decrease in health-related quality of life (HRQoL). Recent studies emphasize the importance of addressing HRQoL in ED treatment, particularly in the context of social isolation and loneliness, but this aspect is currently poorly evaluated.

Methods

A sample of 220 people with an ED was enrolled for the study from different centers specialized in the treatment of ED and compared to 151 people from the general population. Different validated questionnaires were used to evaluate eating psychopathology, HRQoL, and loneliness. Partial correlation analyzes adjusted for marital status and regressions were used to evaluate the relationships between constructs and the differences between groups.

Results

Higher feelings of loneliness were associated with a poorer HRQoL in patients and controls. In the ED group, both loneliness and eating psychopathology were significant predictors of HRQoL. Meanwhile, the duration of the disorder predicted HRQoL specifically in underweight patients, and BMI was a predictor of HRQoL in individuals with normal or above-normal weight.

Conclusions

These findings highlight the importance of considering both HRQoL and loneliness in EDs, particularly among younger individuals. This approach aligns with the increasing focus on the role of interpersonal relationships in the recovery process. Additionally, the data confirm a link between weight and loneliness, suggesting that this connection, especially the differences between underweight patients and those of other weights, deserves further investigation.
Close, yet so far away: a phenomenology of the praecox feeling in the diagnosis of schizophrenia as intercorporeal alienness
Authors: Vial Iván , Moskalewicz Marcin , Szuła Anastazja , Schwartz Michael A. , Fuchs Thomas
Published: October 2024

Debates concerning the reliability and validity of operationalized criteria and diagnostic tools have surrounded the issue of schizophrenia diagnosis and clinical decision-making related to the disorder. The notion of the praecox feeling (PF) has played a prominent role in the discussions as an example of the possibility of a rapid and potentially valid diagnosis based solely on “intuition” or a peculiar emotional experience or impression arising in a physician during an interaction with a patient with schizophrenia. In this paper, we argue that PF is enabled by the (phenomenologically understood) intercorporeal dimension of the clinical encounter. Intercorporeality in this sense denotes intertwinement between embodied expressions that may lead to feelings of connection but also, as in the case of PF, of disconnection and strangeness—the experience of alienness. Following Waldenfels, alienness ranges from the average social encounter to more extreme and peculiar forms—such as PF. To prove our point, we analyze the metaphors used by physicians in various cultural contexts (the United States, the United Kingdom, and Poland) to express the apparently ineffable experience of the PF. We focus on two dominant metaphors of distance: the first expressing spatial distance by referring to an “object in-between” the physician and the patient and the second expressing mental distance by referring to the “other-worldliness” of the patient. We interpret the object in-between metaphors as reflecting the sense of separateness and the other-worldliness metaphors as reflecting the sense of strangeness, with both meanings unified in the notion of “close remoteness.” Such unsettling but speculation-provoking feeling of close remoteness may be rendered by the concept of “the eerie” (Mark Fisher). We conclude that metaphor and phenomenological analysis facilitate an understanding of the experiential profile of PF in the clinical encounter, outlining relevant clinical implications.
Analysis of the status quo and clinical influencing factors of the social cognitive impairment in deficit schizophrenia
Authors: Chengbing Huang , Jia Wang , Lirong Zhuang , Tingting Zhu , Yanling Song , Taipeng Sun , Xiangrong Zhang
Published: October 2024

Background

Due to the high heterogeneity of schizophrenia, the factors influencing social cognitive impairment are controversial. The purpose of this study was to investigate the social cognitive dysfunction of deficit schizophrenia (DS), and to explore its clinical impact on the clinical characteristics and neurocognitive function assessment results.

Methods

This study involved 100 DS patients, 100 non-deficit schizophrenia (NDS) patients, and 100 healthy controls (HC). Social cognitive functions were assessed using the Eye Complex Emotion Discrimination Task (ECEDT), Game of Dice Task (GDT), and Iowa Gambling Task (IGT), while neurocognitive functions were examined using the Clock Drawing Task (CDT), the Verbal Fluency Task (VFT), Digit Span Test (DST), Stroop Color-word Test (SCWT), and Trail Making Test (TMT). We analyzed the differences in cognitive function among the three groups of patients and the correlation between cognitive function assessment results and Positive and Negative Syndrome Scale (PANSS) scores.

Results

Comparison of neurocognitive functions among the three groups through CDT, VFT, DST, SCWT, and TMT revealed that in the values of these tests in the DS group differed significantly from those of the NDS and HC groups. However, the DSB of the NDS group was lower and the TMT results were significantly higher than those of the HC group. In the DS group, ECEDT emotion recognition was positively correlated with stroop colors and stroop interference; the score of gender recognition was positively correlated with VFT, DSF, and SCWT, and TMT-B; the total time spent was positively correlated with TMT; The GDT risky option was negatively correlated with VFT, DST, stroop word, and stroop interference; the negative feedback utilization was negatively correlated with PANSS-Negative; TMT was positively correlated with VFT; IGT was positively correlated with CDT, VFT, DST, and SCWT, but it was negatively correlated with PANSS-Negative and TMT, with statistically significant.

Conclusion

There are significant social cognitive impairments in the perception of social information, judgment and resolution of social problems in deficit schizophrenia, which are closely related to negative symptoms and multidimensional neurocognitive dysfunction such as attention, learning, memory, brain information processing speed, cognitive flexibility, and functional executive power.
Public satisfaction with the Ethiopian healthcare system: a mixed methods approach
Authors: Getahun Genanew Kassie , Demissie Bemnet Dires , Baraki Semere Gebremariam
Published: October 2024

Introduction

The satisfaction of the public with the healthcare system of Ethiopia is a crucial but unanswered question. This is an essential issue since recent trends indicate that the demographic and epidemiological makeup of the population is changing. Therefore, the aim of this study was to assess the overall satisfaction of the public with the Ethiopian healthcare system in Addis Ababa, Ethiopia, in 2022.

Methods

A community-based convergent parallel mixed methods study was conducted. Bivariable and multivariable logistic regression analyses were used to determine the factors associated with public satisfaction. A 95% confidence interval along with a p < 0.05 was deemed sufficient to declare a significant association. For the qualitative component, we used thematic analysis.

Results

The vast majority, 77.2% (95% CI: 76.18–78.22%), of respondents were dissatisfied with the existing healthcare system. Moreover, Community-Based Health Insurance (CBHI) enrollment (2.35; 95% CI: 1.32–4.19), poor linkage to social capital (0.46; 95% CI: 0.25–0.83), poor access to healthcare services (0.39; 95% CI: 0.21–0.76), and absence of satisfactory responses to complaints (0.11; 95% CI: 0.04–0.27) were significantly associated with public dissatisfaction.

Conclusion

Public satisfaction with the Ethiopian healthcare system is notably low and is affected by various factors, including enrollment in the CBHI, linkage to social capital, accessibility to healthcare, and satisfactory response to issues. Therefore, the Ethiopian government should focus on expanding CBHI coverage, improving access to healthcare services, and developing an immediate compliant solution strategy.
Paralysis caused by dinotefuran at environmental concentration via interfering the Ca2+–ROS–mitochondria pathway in Chironomus kiiensis
Authors: Wei Fenghua , Gu Weiwen , Zhang Fengru , Wu Shuangxin
Published: October 2024

Introduction

Dinotefuran as the third-generation of neonicotinoid insecticides is extensively used in agriculture worldwide, posing a potential toxic threat to non-target animals and humans. However, the chronic toxicity mechanism related to mitochondria damage of dinotefuran to non-target animals at environmental concentration is unclear.

Methods

In this study, the mitochondria damage and oxidative stress of dinotefuran on Chironomus kiiensis were investigated at environmental concentrations by long-term exposure. At the same time, relevant gene expressions of these toxicity indexes were measured as sensitive ecotoxicity biomarkers to reflect the toxic effects of dinotefuran on Chironomidae.

Results

Our present study showed that chronic exposure to environmental concentrations of dinotefuran resulted in behavioral inhibition in the larvae of Chironomidae. For burrowing inhibition of 10 days, the lowest observed-effect concentration (LOEC) and 50% inhibitory concentration (IC50) were 0.01 (0.01–0.04) and 0.60 (0.44–0.82) μg/L, respectively. Dinotefuran promoted the release of intracellular calcium ions (Ca2+) in Chironomidae via dysregulating the gene expressions of atp2b, camk ii, and calm. Subsequently, the disruption of the Ca2+ signaling pathway induced oxidative stress by raising reactive oxygen species (ROS), hydrogen peroxide (H2O2), and malonaldehyde (MDA) levels. Thus, the over-release of Ca2+ and ROS disordered the normal functioning of mitochondrial-related pathways by dysregulating the expressions of mitochondria-related genes of atpef0a, sdha, and cyt b.

Conclusion

Our findings showed that low environmental concentrations of dinotefuran caused paralysis of the midge via interfering the Ca2+–ROS–mitochondria pathway. These results provided data support for assessing the potential environmental risk of dinotefuran.
Blood DNA methylation of CRF and its association with amygdala volume and mood in Cushing’s syndrome
Authors: Lee Richard S. , Santos Alicia , Garrison-Desany Henri , Aulinas Anna , Carey Jenny L. , Vives-Gilabert Yolanda , Cox Olivia H. , Cuilan Gabriel , Webb Susan M. , Resmini Eugenia
Published: October 2024

Objective

The impact of chronic exposure to stress or glucocorticoids on psychiatric symptoms has been exemplified by cases of iatrogenic or endogenous hypercortisolism such as Cushing’s syndrome (CS). The amygdala plays an important role in mediating both stress and affective responses, and one of the key factors that link stress response and psychiatric symptoms is the corticotropin-releasing factor (CRF). Epigenetic changes, especially those occurring on CpG dinucleotides in DNA of glucocorticoid target genes in blood, have been previously implicated as potential predictors of glucocorticoid-related events in the central nervous system (CNS). In this study, we examined amygdala volume and mood symptoms in CS patients and aimed at evaluating whether these parameters were associated with blood DNA methylation of CRF.

Methods

In this cross-sectional study, 32 CS patients and 32 healthy controls matched for age, sex, and years of education underwent an MRI scan, a Beck Depression Inventory-II, and a State-Trait Anxiety Inventory. Genomic DNA extracted from total leukocytes were used for DNA methylation analysis of several CpG dinucleotides at the CRF promoter region.

Results

Significant associations between CRF methylation vs. amygdala volume (CpG-1, P = 0.006) and depression scores (CpG-2, P = 0.01) were found. To assess whether the promoter CpG methylation has functional consequences, we examined RNA and DNA extracted from non-CS, postmortem amygdala tissues. A significant association between CpG methylation and gene expression (CpG-1, P = 0.004) was observed.

Conclusion

These results demonstrate that methylation levels of the CRF promoter CpGs are associated with amygdala volume in CS and related mood symptoms. Methylation levels may also be associated with CRF expression. This finding supports the feasibility of using epigenetic patterns in blood as a surrogate for assessing GC-related pathologies in the brain.