bluuebunny/crossref_metadata_2025 huggingface dataset:
Hepatotoxicity of Pyrrolizidine Alkaloid Compound Intermedine: Comparison with Other Pyrrolizidine Alkaloids and Its Toxicological Mechanism
Authors: Wang Ziqi , Han Haolei , Wang Chen , Zheng Qinqin , Chen Hongping , Zhang Xiangchun , Hou Ruyan
Published: December 2021

Pyrrolizidine alkaloids (PAs) are common secondary plant compounds with hepatotoxicity. The consumption of herbal medicines and herbal teas containing PAs is one of the main causes of hepatic sinusoidal obstruction syndrome (HSOS), a potentially life-threatening condition. The present study aimed to reveal the mechanism underlying the cytotoxicity of intermedine (Im), the main PA in Comfrey. We evaluated the toxicity of the retronecine-type PAs with different structures to cell lines derived from mammalian tissues, including primary mouse hepatocytes, human hepatocytes (HepD), mouse hepatoma-22 (H22) and human hepatocellular carcinoma (HepG2) cells. The cytotoxicity of Im to hepatocyte was evaluated by using cell counting kit-8 assay, colony formation experiment, wound healing assay and dead/live fluorescence imaging. In vitro characterization showed that these PAs were cytotoxic and induced cell apoptosis in a dose-dependent manner. We also demonstrated that Im induced cell apoptosis by generating excessive reactive oxygen species (ROS), changing the mitochondrial membrane potential and releasing cytochrome c (Cyt c) before activating the caspase-3 pathway. Importantly, we directly observed the destruction of the cell mitochondrial structure after Im treatment through transmission electron microscopy (TEM). This study provided the first direct evidence of Im inducing hepatotoxicity through mitochondria-mediated apoptosis. These results supplemented the basic toxicity data of PAs and facilitated the comprehensive and systematic evaluation of the toxicity caused by PA compounds.
Screening for Chagas Disease during Pregnancy in the United States—A Literature Review
Authors: Livingston Elizabeth G. , Duggal Ryan , Dotters-Katz Sarah
Published: December 2021

Obstetrician-gynecologists in the United States have little clinical experience with the epidemiology, pathophysiology, diagnosis, and treatment of Chagas disease. The number of US parturients born in Central and South America has continued to increase over the last 20 years, making US obstetricians more and more likely to care for Chagas-infected mothers who may never be identified until dealing with long-term consequences of the disease. A literature search demonstrates that few US obstetric care providers recognize the risk of vertical transmission for the neonate and the missed opportunity of infant treatment to decrease disease prevalence. Most women will be asymptomatic during pregnancy, as will their neonates, making routine laboratory screening a necessity for the identification of at-risk neonates. While the benefits of treating asymptomatic women identified in pregnancy are not as clear as the benefits for the infants, future health screenings for evidence of the progression of Chagas disease may be beneficial to these families. The literature suggests that screening for Chagas in pregnancy in the US can be done in a cost-effective way. When viewed through an equity lens, this condition disproportionately affects families of lower socioeconomic means. Improved education of healthcare providers and appropriate resources for diagnosis and treatment can improve this disparity in health outcomes.
Active Case Finding for Tuberculosis in India: A Syntheses of Activities and Outcomes Reported by the National Tuberculosis Elimination Programme
Authors: Burugina Nagaraja Sharath , Thekkur Pruthu , Satyanarayana Srinath , Tharyan Prathap , Sagili Karuna D. , Tonsing Jamhoih , Rao Raghuram , Sachdeva Kuldeep Singh
Published: December 2021

India launched a national community-based active TB case finding (ACF) campaign in 2017 as part of the strategic plan of the National Tuberculosis Elimination Programme (NTEP). This review evaluated the outcomes for the components of the ACF campaign against the NTEP’s minimum indicators and elicited the challenges faced in implementation. We supplemented data from completed pretested data proformas returned by ACF programme managers from nine states and two union territories (for 2017–2019) and five implementing partner agencies (2013–2020), with summary national data on the state-wise ACF outcomes for 2018–2020 published in annual reports by the NTEP. The data revealed variations in the strategies used to map and screen vulnerable populations and the diagnostic algorithms used across the states and union territories. National data were unavailable to assess whether the NTEP indicators for the minimum proportions identified with presumptive TB among those screened (5%), those with presumptive TB undergoing diagnostic tests (>95%), the minimum sputum smear positivity rate (2% to 3%), those with negative sputum smears tested with chest X-rays or CBNAAT (>95%) and those diagnosed through ACF initiated on anti-TB treatment (>95%) were fulfilled. Only 30% (10/33) of the states in 2018, 23% (7/31) in 2019 and 21% (7/34) in 2020 met the NTEP expectation that 5% of those tested through ACF would be diagnosed with TB (all forms). The number needed to screen to diagnose one person with TB (NNS) was not included among the NTEP’s programme indicators. This rough indicator of the efficiency of ACF varied considerably across the states and union territories. The median NNS in 2018 was 2080 (interquartile range or IQR 517–4068). In 2019, the NNS was 2468 (IQR 1050–7924), and in 2020, the NNS was 906 (IQR 108–6550). The data consistently revealed that the states that tested a greater proportion of those screened during ACF and used chest X-rays or CBNAAT (or both) to diagnose TB had a higher diagnostic yield with a lower NNS. Many implementation challenges, related to health systems, healthcare provision and difficulties experienced by patients, were elicited. We suggest a series of strategic interventions addressing the implementation challenges and the six gaps identified in ACF outcomes and the expected indicators that could potentially improve the efficacy and effectiveness of community-based ACF in India.
Identification of African Swine Fever Virus Transcription within Peripheral Blood Mononuclear Cells of Acutely Infected Pigs
Authors: Olesen Ann Sofie , Kodama Miyako , Lohse Louise , Accensi Francesc , Rasmussen Thomas Bruun , Lazov Christina M. , Limborg Morten T. , Gilbert M. Thomas P. , Bøtner Anette , Belsham Graham J.
Published: December 2021

African swine fever virus (ASFV) has become widespread in Europe, Asia and elsewhere, thereby causing extensive economic losses. The viral genome includes nearly 200 genes, but their expression within infected pigs has not been well characterized previously. In this study, four pigs were infected with a genotype II strain (ASFV POL/2015/Podlaskie); blood samples were collected before inoculation and at both 3 and 6 days later. During this period, a range of clinical signs of infection became apparent in the pigs. From the blood, peripheral blood mononuclear cells (PBMCs) were isolated. The transcription of the ASFV genes was determined using RNAseq on poly(A)+ mRNAs isolated from these cells. Only very low levels of virus transcription were detected in the PBMCs at 3 days post-inoculation (dpi) but, at 6 dpi, extensive transcription was apparent. This was co-incident with a large increase in the level of ASFV DNA within these cells. The pattern of the virus gene expression was very reproducible between the individual pigs. Many highly expressed genes have undefined roles. Surprisingly, some genes with key roles in virus replication were expressed at only low levels. As the functions of individual genes are identified, information about their expression becomes important for understanding their contribution to virus biology.
Genomic Epidemiology and Heterogeneity of SRLV in Italy from 1998 to 2019
Authors: Bazzucchi Moira , Pierini Ilaria , Gobbi Paola , Pirani Silvia , Torresi Claudia , Iscaro Carmen , Feliziani Francesco , Giammarioli Monica
Published: December 2021

Small ruminant lentiviruses (SRLV) are viruses that retro-transcribe RNA to DNA and show high rates of genetic variability. SRLV affect animals with strains specific for each host species (sheep or goats), resulting in a series of clinical manifestations depending on the virulence of the strain, the host’s genetic background and farm production system. The aim of this work was to present an up-to-date overview of the genomic epidemiology and genetic diversity of SRLV in Italy over time (1998–2019). In this study, we investigated 219 SRLV samples collected from 17 different Italian regions in 178 geographically distinct herds by CEREL. Our genetic study was based on partial sequencing of the gag-pol gene (800 bp) and phylogenetic analysis. We identified new subtypes with high heterogeneity, new clusters and recombinant forms. The genetic diversity of Italian SRLV strains may have diagnostic and immunological implications that affect the performance of diagnostic tools. Therefore, it is extremely important to increase the control of genomic variants to improve the control measures.
Bacteriophages as an Alternative Method for Control of Zoonotic and Foodborne Pathogens
Authors: Alomari Mohammed Mijbas Mohammed , Dec Marta , Urban-Chmiel Renata
Published: December 2021

The global increase in multidrug-resistant infections caused by various pathogens has raised concerns in human and veterinary medicine. This has renewed interest in the development of alternative methods to antibiotics, including the use of bacteriophages for controlling bacterial infections. The aim of this review is to present potential uses of bacteriophages as an alternative to antibiotics in the control of bacterial infections caused by multidrug-resistant bacteria posing a risk to humans, with particular emphasis on foodborne and zoonotic pathogens. A varied therapeutic and immunomodulatory (activation or suppression) effect of bacteriophages on humoral and cellular immune response mechanisms has been demonstrated. The antibiotic resistance crisis caused by global antimicrobial resistance among bacteria creates a compelling need for alternative safe and selectively effective antibacterial agents. Bacteriophages have many properties indicating their potential suitability as therapeutic and/or prophylactic agents. In many cases, bacteriophages can also be used in food quality control against microorganisms such as Salmonella, Escherichia coli, Listeria, Campylobacter and others. Future research will provide potential alternative solutions using bacteriophages to treat infections caused by multidrug-resistant bacteria.
Human Cytomegalovirus Replication and Infection-Induced Syncytia Formation in Labial, Foreskin, and Fetal Lung Fibroblasts
Authors: Aguiar Alexis , Galinato Melissa , Bradley Silva Maite’ , Toth Bryant , McVoy Michael A. , Hertel Laura
Published: December 2021

Only a handful of cell types, including fibroblasts, epithelial, and endothelial cells, can support human cytomegalovirus (CMV) replication in vitro, in striking contrast to the situation in vivo. While the susceptibility of epithelial and endothelial cells to CMV infection is strongly modulated by their anatomical site of origin, multiple CMV strains have been successfully isolated and propagated on fibroblasts derived from different organs. As oral mucosal cells are likely involved in CMV acquisition, we sought to evaluate the ability of infant labial fibroblasts to support CMV replication, compared to that of commonly used foreskin and fetal lung fibroblasts. No differences were found in the proportion of cells initiating infection, or in the amounts of viral progeny produced after exposure to the fibroblast-adapted CMV strain AD169 or to the endothelial cell-adapted strain TB40/E. Syncytia formation was, however, significantly enhanced in infected labial and lung fibroblasts compared to foreskin-derived cells, and did not occur after infection with AD169. Together, these data indicate that fibroblast populations derived from different tissues are uniformly permissive to CMV infection but retain phenotypic differences of potential importance for infection-induced cell–cell fusion, and ensuing viral spread and pathogenesis in different organs.
Disease Tolerance during Viral-Bacterial Co-Infections
Authors: Barman Tarani Kanta , Metzger Dennis W.
Published: December 2021

Disease tolerance has emerged as an alternative way, in addition to host resistance, to survive viral-bacterial co-infections. Disease tolerance plays an important role not in reducing pathogen burden, but in maintaining tissue integrity and controlling organ damage. A common co-infection is the synergy observed between influenza virus and Streptococcus pneumoniae that results in superinfection and lethality. Several host cytokines and cells have shown promise in promoting tissue protection and damage control while others induce severe immunopathology leading to high levels of morbidity and mortality. The focus of this review is to describe the host cytokines and innate immune cells that mediate disease tolerance and lead to a return to host homeostasis and ultimately, survival during viral-bacterial co-infection.
The Viral Class II Membrane Fusion Machinery: Divergent Evolution from an Ancestral Heterodimer
Authors: Guardado-Calvo Pablo , Rey Félix A.
Published: December 2021

A key step during the entry of enveloped viruses into cells is the merger of viral and cell lipid bilayers. This process is driven by a dedicated membrane fusion protein (MFP) present at the virion surface, which undergoes a membrane–fusogenic conformational change triggered by interactions with the target cell. Viral MFPs have been extensively studied structurally, and are divided into three classes depending on their three-dimensional fold. Because MFPs of the same class are found in otherwise unrelated viruses, their intra-class structural homology indicates horizontal gene exchange. We focus this review on the class II fusion machinery, which is composed of two glycoproteins that associate as heterodimers. They fold together in the ER of infected cells such that the MFP adopts a conformation primed to react to specific clues only upon contact with a target cell, avoiding premature fusion in the producer cell. We show that, despite having diverged in their 3D fold during evolution much more than the actual MFP, the class II accompanying proteins (AP) also derive from a distant common ancestor, displaying an invariant core formed by a β-ribbon and a C-terminal immunoglobulin-like domain playing different functional roles—heterotypic interactions with the MFP, and homotypic AP/AP contacts to form spikes, respectively. Our analysis shows that class II APs are easily identifiable with modern structural prediction algorithms, providing useful information in devising immunogens for vaccine design.
Dynamic, but Not Necessarily Disordered, Human-Virus Interactions Mediated through SLiMs in Viral Proteins
Authors: Elkhaligy Heidy , Balbin Christian A. , Gonzalez Jessica L. , Liberatore Teresa , Siltberg-Liberles Jessica
Published: December 2021

Most viruses have small genomes that encode proteins needed to perform essential enzymatic functions. Across virus families, primary enzyme functions are under functional constraint; however, secondary functions mediated by exposed protein surfaces that promote interactions with the host proteins may be less constrained. Viruses often form transient interactions with host proteins through conformationally flexible interfaces. Exposed flexible amino acid residues are known to evolve rapidly suggesting that secondary functions may generate diverse interaction potentials between viruses within the same viral family. One mechanism of interaction is viral mimicry through short linear motifs (SLiMs) that act as functional signatures in host proteins. Viral SLiMs display specific patterns of adjacent amino acids that resemble their host SLiMs and may occur by chance numerous times in viral proteins due to mutational and selective processes. Through mimicry of SLiMs in the host cell proteome, viruses can interfere with the protein interaction network of the host and utilize the host-cell machinery to their benefit. The overlap between rapidly evolving protein regions and the location of functionally critical SLiMs suggest that these motifs and their functional potential may be rapidly rewired causing variation in pathogenicity, infectivity, and virulence of related viruses. The following review provides an overview of known viral SLiMs with select examples of their role in the life cycle of a virus, and a discussion of the structural properties of experimentally validated SLiMs highlighting that a large portion of known viral SLiMs are devoid of predicted intrinsic disorder based on the viral SLiMs from the ELM database.
A Rapid Point-of-Care Test for the Serodiagnosis of Hepatitis Delta Virus Infection
Authors: Lempp Florian A. , Roggenbach Imme , Nkongolo Shirin , Sakin Volkan , Schlund Franziska , Schnitzler Paul , Wedemeyer Heiner , Le Gal Frédéric , Gordien Emmanuel , Yurdaydin Cihan , Urban Stephan
Published: December 2021

Hepatitis Delta virus (HDV) is a satellite of the Hepatitis B virus (HBV) and causes severe liver disease. The estimated prevalence of 15–20 million infected people worldwide may be underestimated as international diagnostic guidelines are not routinely followed. Possible reasons for this include the limited awareness among healthcare providers, the requirement for costly equipment and specialized training, and a lack of access to reliable tests in regions with poor medical infrastructure. In this study, we developed an HDV rapid test for the detection of antibodies against the hepatitis delta antigen (anti-HDV) in serum and plasma. The test is based on a novel recombinant large hepatitis delta antigen that can detect anti-HDV in a concentration-dependent manner with pan-genotypic activity across all known HDV genotypes. We evaluated the performance of this test on a cohort of 474 patient samples and found that it has a sensitivity of 94.6% (314/332) and a specificity of 100% (142/142) when compared to a diagnostic gold-standard ELISA. It also works robustly for a broad range of anti-HDV titers. We anticipate this novel HDV rapid test to be an important tool for epidemiological studies and clinical diagnostics, especially in regions that currently lack access to reliable HDV testing.
Drugs of Abuse and Their Impact on Viral Pathogenesis
Authors: Blackard Jason , Sherman Kenneth
Published: December 2021

Commonly misused substances such as alcohol, cocaine, heroin, methamphetamine, and opioids suppress immune responses and may impact viral pathogenesis. In recent years, illicit use of opioids has fueled outbreaks of several viral pathogens, including the human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV). This review focuses on the myriad of mechanisms by which drugs of abuse impact viral replication and disease progression. Virus–drug interactions can accelerate viral disease progression and lead to increased risk of virus transmission.
Marburg Virus Persistence on Fruit as a Plausible Route of Bat to Primate Filovirus Transmission
Authors: Amman Brian R. , Schuh Amy J. , Albariño César G. , Towner Jonathan S.
Published: December 2021

Marburg virus (MARV), the causative agent of Marburg virus disease, emerges sporadically in sub-Saharan Africa and is often fatal in humas. The natural reservoir for this zoonotic virus is the frugivorous Egyptian rousette bat (Rousettus aegyptiacus) that when infected, sheds virus in the highest amounts in oral secretions and urine. Being fruit bats, these animals forage nightly for ripened fruit throughout the year, including those types often preferred by humans. During feeding, they continually discard partially eaten fruit on the ground that could then be consumed by other Marburg virus susceptible animals or humans. In this study, using qRT-PCR and virus isolation, we tested fruit discarded by Egyptian rousette bats experimentally infected with a natural bat isolate of Marburg virus. We then separately tested viral persistence on fruit varieties commonly cultivated in sub-Saharan Africa using a recombinant Marburg virus expressing the fluorescent ZsGreen1. Marburg virus RNA was repeatedly detected on fruit in the food bowls of the infected bats and viable MARV was recovered from inoculated fruit for up to 6 h.
COVID-19 In-Hospital Mortality in People with Diabetes Is Driven by Comorbidities and Age—Propensity Score-Matched Analysis of Austrian National Public Health Institute Data
Authors: Aziz Faisal , Aberer Felix , Bräuer Alexander , Ciardi Christian , Clodi Martin , Fasching Peter , Karolyi Mario , Kautzky-Willer Alexandra , Klammer Carmen , Malle Oliver , Pawelka Erich , Pieber Thomas , Peric Slobodan , Ress Claudia , Schranz Michael , Sourij Caren , Stechemesser Lars , Stingl Harald , Stöcher Hannah , Stulnig Thomas , Tripolt Norbert , Wagner Michael , Wolf Peter , Zitterl Andreas , Reisinger Alexander Christian , Siller-Matula Jolanta , Hummer Michael , Moser Othmar , von-Lewinski Dirk , Eller Philipp , Kaser Susanne , Sourij Harald
Published: December 2021

Background: It is a matter of debate whether diabetes alone or its associated comorbidities are responsible for severe COVID-19 outcomes. This study assessed the impact of diabetes on intensive care unit (ICU) admission and in-hospital mortality in hospitalized COVID-19 patients. Methods: A retrospective analysis was performed on a countrywide cohort of 40,632 COVID-19 patients hospitalized between March 2020 and March 2021. Data were provided by the Austrian data platform. The association of diabetes with outcomes was assessed using unmatched and propensity-score matched (PSM) logistic regression. Results: 12.2% of patients had diabetes, 14.5% were admitted to the ICU, and 16.2% died in the hospital. Unmatched logistic regression analysis showed a significant association of diabetes (odds ratio [OR]: 1.24, 95% confidence interval [CI]: 1.15–1.34, p < 0.001) with in-hospital mortality, whereas PSM analysis showed no significant association of diabetes with in-hospital mortality (OR: 1.08, 95%CI: 0.97–1.19, p = 0.146). Diabetes was associated with higher odds of ICU admissions in both unmatched (OR: 1.36, 95%CI: 1.25–1.47, p < 0.001) and PSM analysis (OR: 1.15, 95%CI: 1.04–1.28, p = 0.009). Conclusions: People with diabetes were more likely to be admitted to ICU compared to those without diabetes. However, advanced age and comorbidities rather than diabetes itself were associated with increased in-hospital mortality in COVID-19 patients.
Widespread Circulation of Flaviviruses in Horses and Birds in Northeastern Spain (Catalonia) between 2010 and 2019
Authors: Napp Sebastian , Llorente Francisco , Beck Cécile , Jose-Cunilleras Eduard , Soler Mercè , Pailler-García Lola , Amaral Rayane , Aguilera-Sepúlveda Pilar , Pifarré Maria , Molina-López Rafael , Obón Elena , Nicolás Olga , Lecollinet Sylvie , Jiménez-Clavero Miguel Ángel , Busquets Núria
Published: December 2021

The surveillance for West Nile virus (WNV) in Catalonia (northeastern Spain) has consistently detected flaviviruses not identified as WNV. With the aim of characterizing the flaviviruses circulating in Catalonia, serum samples from birds and horses collected between 2010 and 2019 and positive by panflavivirus competition ELISA (cELISA) were analyzed by microneutralization test (MNT) against different flaviviruses. A third of the samples tested were inconclusive by MNT, highlighting the limitations of current diagnostic techniques. Our results evidenced the widespread circulation of flaviviruses, in particular WNV, but also Usutu virus (USUV), and suggest that chicken and horses could serve as sentinels for both viruses. In several regions, WNV and USUV overlapped, but no significant geographical aggregation was observed. Bagaza virus (BAGV) was not detected in birds, while positivity to tick-borne encephalitis virus (TBEV) was sporadically detected in horses although no endemic foci were observed. So far, no human infections by WNV, USUV, or TBEV have been reported in Catalonia. However, these zoonotic flaviviruses need to be kept under surveillance, ideally within a One Health framework.
Determination of Speed-Dependent Roadway Luminance for an Adequate Feeling of Safety at Nighttime Driving
Authors: Erkan Anil , Babilon Sebastian , Hoffmann David , Singer Timo , Vitkov Tsoni , Khanh Tran Quoc
Published: December 2021

The purpose of this work is to determine as a function of velocity the minimal roadway luminance that is required to be judged as being bright enough for a driver to perform a nighttime driving task with an adequate feeling of safety. In this context, it shall also be evaluated which areas of the vehicle forefield are most crucial for the driver’s general brightness perception. A field study with 23 subjects and dimmable LED headlights was conducted, in which the subjects were given the task to assess their perceived brightness for different luminance levels caused by the headlights’ low-beam distribution in the vehicle’s forefield on a 5-step rating scale. The experiments were repeated for three different driving velocities of 0 km h−1 (static case), 30 km h−1, and 60 km h−1, respectively. Results for the static case indicate that, for the roadway to be perceived as bright enough by 50% of the subjects, an average roadway luminance of 0.88 cd m−2 is required in an area up to 32 m in front of the vehicle. Furthermore, a significant effect of driving speed is observed. For example, at 60 km h−1, the luminance must be increased to 1.54 cd m−2 to be still perceived as sufficiently bright by 50% of the subjects.
Full-Scale Blast Tests on a Conventionally Designed Three-Story Steel Braced Frame with Composite Floor Slabs
Authors: Hadjioannou Michalis , McKay Aldo E. , Benshoof Phillip C.
Published: December 2021

This paper summarizes the findings of two full-scale blasts tests on a steel braced frame structure with composite floor slabs, which are representative of a typical office building. The aim of this research study was to experimentally characterize the behavior of conventionally designed steel braced frames to blast loads when enclosed with conventional and blast-resistant façade. The two tests involved a three-story, steel braced frame with concentrical steel braces, which are designed to resist typical gravity and wind loads without design provisions for blast or earthquake loads. During the first blast test, the structure was enclosed with a typical, non-blast-resistant, curtainwall façade, and the steel frame sustained minimal damage. For the second blast test, the structure was enclosed with a blast-resistant façade, which resulted in higher damage levels with some brace connections rupturing, but the building did not collapse. Observations from the test program indicate the appreciable reserved capacity of steel brace frame structures to resist blast loads.
Passive Treatment for Acid Mine Drainage Partially Restores Microbial Community Structure in Different Stream Habitats
Authors: Neff Alexis N. , DeNicola Dean M. , Maltman Chris
Published: December 2021

The assessment of the degree to which biological communities in streams impaired by acid mine drainage (AMD) are restored by passive treatment has focused primarily on eukaryotic-cell organisms and microbial processes. The responses of microbial community structure to passive treatment have received much less attention, even though functional processes such as nutrient cycling and organic matter decomposition depend on taxonomic composition. Our objective was to determine the degree to which passive treatment restored microbial communities in three types of habitats: aqueous, leaf, and sediment. To assess their recovery, we compared the community composition in these habitats based on 16S rRNA gene sequencing at three different stream sites: an untreated AMD site (U), a remediated site below AMD passive treatment (T), and an unimpaired reference site (R). The acidity, conductivity, and soluble metal concentrations at T were found to be elevated compared to R, but generally 1–2 orders of magnitude less than at U. Microbial community composition was found to be synergistically affected by habitat type and AMD impact, with the similarity among communities in the three habitats increasing with the severity of the AMD. Sediment- and leaf-associated microbial communities at U were characterized by taxa that are tolerant to severe AMD. The absence of the nitrogen oxidizing bacterium Nitrospira in sediment communities at T and U was found to correspond to higher NH4+ concentrations compared to R, possibly because of the presence of iron oxyhydroxide precipitate. In contrast, the microbial composition was found to be similar between the T and R sites for both aqueous and leaf communities, indicating that passive treatment was more able to restore these communities to the reference condition than sediment communities. The remediation of AMD streams should consider the habitat-specific responses of microbial community composition and be guided by future studies that empirically couple changes in taxonomic composition to measured functional processes.
Hydraulic and Structural Analysis of Complex Cross-Section Reinforced Concrete Pipes to Improve Sewage Flow in a Combined Sewer System
Authors: Ji Hyon Wook , Kang Jeong-Hee , Koo Dan Daehyun , Yoo Sung Soo
Published: December 2021

A complex cross-section reinforced concrete pipe that combines a sub-pipe for the flow of sewage in dry weather and a main pipe for the flow of rainwater was developed to reduce sedimentation of the combined sewer system in dry weather. The sub-pipe was designed, considering the flow velocity, constructability, and maintenance. By fitting the sewage data in the dry weather to the normal distribution, the ratio of the cross-sectional area of sewage flow to that of the pipe was determined to be approximately 0.418, which could cover 99.85% of the sewage volume of the target site. Based on this ratio, the diameter of the sub-pipe corresponding to the combined sewer system with a pipe diameter between 450 and 1300 mm was determined. The hydraulic performance analysis results showed that the flow velocity increased by 11 to 12% compared to the circular pipe based on the full sub-pipe and by more than 15% depending on the water level. The shear stress increased by more than 16.5%, and higher tractive force was observed. Structural safety was determined as the crack load and failure load far exceeded the minimum criteria, thereby verifying the feasibility and field applicability of the complex cross-section reinforced concrete pipe.
The Application of a Self-Organizing Model for the Estimation of Crop Water Stress Index (CWSI) in Soybean with Different Watering Levels
Authors: Anda Angela , Simon-Gáspár Brigitta , Soós Gábor
Published: December 2021

A field experiment was conducted with soybean to observe evapotranspiration (ET) and crop water stress index (CWSI) with three watering levels at Keszthely, Hungary, during the growing seasons 2017–2020. The three different watering levels were rainfed, unlimited, and water stress in flowering. Traditional and converted evapotranspirometers documented water stress levels in two soybean varieties (Sinara, Sigalia), with differing water demands. ET totals with no significant differences between varieties varied from 291.9 to 694.9 mm in dry, and from 205.5 to 615.6 mm in wet seasons. Theoretical CWSI, CWSIt was computed using the method of Jackson. One of the seasons, the wet 2020 had to be excluded from the CWSIt analysis because of uncertain canopy temperature, Tc data. Seasonal mean CWSIt and Tc were inversely related to water use efficiency. An unsupervised Kohonen self-organizing map (K-SOM) was developed to predict the CWSI, CWSIp based on easily accessible meteorological variables and Tc. In the prediction, the CWSIp of three watering levels and two varieties covered a wide range of index values. The results suggest that CWSIp modelling with the minimum amount of input data provided opportunity for reliable CWSIp predictions in every water treatment (R2 = 0.935–0.953; RMSE = 0.033–0.068 mm, MAE = 0.026–0.158, NSE = 0.336–0.901, SI = 0.095–0.182) that could be useful in water stress management of soybean. However, highly variable weather conditions in the mild continental climate of Hungary might limit the potential of CWSI application. The results in the study suggest that a less than 450 mm seasonal precipitation caused yield reduction. Therefore, a 100–160 mm additional water use could be recommended during the dry growing seasons of the country. The 150 year-long local meteorological data indicated that 6 growing seasons out of 10 are short of precipitation in rainfed soybean.
Cost-Effectiveness of Treatment Wetlands for Nitrogen Removal in Tropical and Subtropical Australia
Authors: Kavehei Emad , Hasan Syezlin , Wegscheidl Carla , Griffiths Matthew , Smart James C. R. , Bueno Carlos , Owen Liz , Akrami Kambez , Shepherd Mel , Lowe Scott , Adame Maria Fernanda
Published: December 2021

Treatment wetlands can reduce nitrogen (N) pollution in waterways. However, the shortage of information on their cost-effectiveness has resulted in their relatively slow uptake in tropical and subtropical Australia, including the catchments of the Great Barrier Reef and Moreton Bay. We assessed the performance of constructed treatment wetlands (CW) and vegetated drains (VD) that treat agricultural runoff, and of sewage treatment plant wetlands (STPW), which polish treated effluent. Treatment performance was estimated as changes in concentration (dissolved inorganic nitrogen, DIN, and total nitrogen, TN; mg L−1) and annual load reductions (kg N ha−1 yr−1). We calculated their cost-effectiveness by comparing their N removal against the costs incurred in their design, construction, and maintenance. Overall, CWs and VDs reduced DIN concentrations by 44% (0.52 to 0.29 mg L−1), and STPW reduced them by 91% (2.3 to 0.2 mg L−1); STPWs also reduced TN concentrations by 72%. The efficiency varied among sites, with the best performing CWs and VDs being those with relatively high inflow concentrations (>0.2 mg L−1 of DIN, >0.7 mg L−1 of TN), low suspended solids, high vegetation cover and high length: width ratio. These high performing CWs and VDs removed N for less than USD 37 kg−1 DIN (AUD 50 kg−1 DIN), less than the end-of-catchment benchmark for the Great Barrier Reef of USD 110 kg−1 DIN (AUD 150 kg−1 DIN). When adequately located, designed, and managed, treatment wetlands can be cost-effective and should be adopted for reducing N in tropical and subtropical Australia.
Channel Migration of the Meandering River Fan: A Case Study of the Okavango Delta
Authors: Yan Xue , Zhang Jinliang , Li Yang , Sun Long
Published: December 2021

Okavango delta is a typical distributive fluvial system, which is composed of a series of sand island-river-swamp networks. River migration in the Okavango delta is analyzed by using satellite images from Google Earth and Alaska Satellite Facility (ASF). Four configuration characterization parameters are selected to depict and measure the meandering river. These four parameters are sinuosity index (S), curvature (C), the difference of along-current deflection angle (Δθ) and expansion coefficient (Km). In the fan, the channel migration is mainly asymmetric. According to geomorphic elements and associated features, Okavango Delta can be subdivided into three zones: axial zone, median zone and distal zone. Under the influence of slope, climate and vegetation, different migration modes are developed in different zones. As the river moves downstream, the sinuosity index of the river on the Okavango Delta decreases downstream. Based on the characteristics of different zones, the sedimentary facies model of a single source distributive fluvial system of a meandering river is proposed. The models of channel migration and sedimentary facies have wide application. This research will not only provide a basis for the prediction of future river channels but will also provide important theoretical guidance for the study of the sedimentary morphology of underground reservoirs.
Enhanced Sediment Denitrification for Nitrogen Removal by Manipulating Water Level in the Lakeshore Zone
Authors: Gao Yangfang , Wang Mingming , Wei Jun , Kong Lingwei , Xu Hui , Shi Wenqing , Zhu Lin
Published: December 2021

Denitrification of sediments is an important way to remove reactive nitrogen in lakeshore zones. In this work, we analyzed sediment denitrification patterns across the shore zone of Lake Taihu and explored their underlying mechanisms using flooding simulation experiments. The results showed that denitrification mainly occurred in the upper sediment layer (0–10 cm) and the denitrification rate was highest at the land–water interface (6.2 mg N/m2h), where there was a frequent rise and fall in the water level. Denitrification was weaker in the lakebed sediments (4.6 mg N/m2h), which were inundated long-term, and in the sediments of the near-shore zone (2.3 mg N/m2h), which were dried out for extended periods. Flooding simulation experiments further indicated a strong positive relationship between sediment denitrification rate and flooding frequency. When the flooding occurred once every 3, 6, 9, 12, or 15 days, the denitrification rate reached 7.6, 5.7, 2.8, 0.9, and 0.6 mg N/m2h, respectively. Frequent flooding caused alternating anoxic and aerobic conditions in sediments, accelerating nitrogen substrate supply and promoting the growth and activity of denitrifying bacteria. Based on these findings, we propose a possible strategy for enhancing sediment denitrification by manipulating the water level, which can help guide nitrogen removal in lakeshore zones.
On the Degradation of Glyphosate by Photocatalysis Using TiO2/Biochar Composite Obtained from the Pyrolysis of Rice Husk
Authors: Le Phuong Thu , Le Duy Ngoc , Nguyen Thi Hue , Bui Huyen Thuong , Pham Le Anh , Nguyen Luong Lam , Nguyen Quoc Son , Nguyen Thu Phuong , Dang Thu Hien , Duong Thi Thuy , Herrmann Marine , Ouillon Sylvain , Le Thi Phuong Quynh , Vo Dieu Linh , Mai Huong , Dinh Thi Mai Thanh
Published: December 2021

In this study, titanium dioxide (TiO2) nanoparticles are immobilized onto rice husk biochar (RHB), as a porous support, for the photodegradation of glyphosate under UV light irradiation. The TiO2/RHB composites are prepared by pyrolysis and the sol-gel method. The SEM, XRD, EDX, and FT-IR results confirm the graphene structure of RHB and the formation of 10.61 nm TiO2 nanoparticles on the catalyst support. The effects of operating conditions, including catalyst dosage (3 g L−1, 5 g L−1, 10 g L−1, and 20 g L−1) and different illumination conditions (9 W lamp, 2 × 9 W lamps), on the removal of glyphosate from aqueous solutions were investigated. The photodegradation efficiency of 15 mg L−1 of commercial glyphosate was up to 99% after 5 h of irradiation at pH 3.0, with a TiO2/RHB dosage of 10 g L−1. However, the mineralization efficiency under this condition was lower than the decomposition efficiency of glyphosate, proving the partial degradation of glyphosate into AMPA and other metabolites after 5 h of reaction.
Degradation of Aqueous CONFIDOR® Pesticide by Simultaneous TiO2 Photocatalysis and Fe-Zeolite Catalytic Ozonation
Authors: Raashid Muhammad , Kazmi Mohsin , Ikhlaq Amir , Iqbal Tanveer , Sulaiman Muhammad , Shakeel Ahmad
Published: December 2021

Due to the importance of water for human survival and scarcity of freshwater resources, wastewater treatment has become very important recently. Some persistent pollutants, such as pesticides, are not removed even after multiple conventional wastewater treatment techniques. Advanced oxidation processes (AOPs) are one of the novel techniques that can be used to treat these persistent compounds. Photocatalytic ozonation is a promising AOP that combines photocatalysis and ozonation for synergistic effects and faster degradation of persistent pollutants. However, usually, only a photocatalyst is used while combining photocatalysis and ozonation. In this work, both a photocatalyst and ozonation catalyst have been simultaneously used for the degradation of commercially available CONFIDOR® pesticide, a Bayer product with Imidacloprid as the active ingredient. TiO2 is employed as a photocatalyst, and Fe-coated Zeolite is employed as an ozonation catalyst. The results show that the reaction rate increases by 1.4 times if both catalysts are used as compared to the use of one photocatalyst only. Almost complete removal (>99%) of pollutant is achieved after 20 min with the simultaneous use of a catalyst when imidacloprid with an initial concentration of 100 mg/L is subjected to 250 W/m2 UV of a wavelength of 253.7 nm and 100 mg/h ozone, where it takes 30 min if only one photocatalyst is used. The paper also explores the effect of initial concentration, UV intensity, catalyst dose and catalyst reuse while also briefly discussing the kinetics and mechanism.
Water Extraction from Fully Polarized SAR Based on Combined Polarization and Texture Features
Authors: Wan Jikang , Wang Jiayi , Zhu Min
Published: December 2021

Given the limited features (for example, the backscattering coefficient threshold range) of single-channel Synthetic Aperture Radar (SAR) images, it is difficult to distinguish ground objects similar to the backscattering coefficients of water bodies. In this paper, two representative research areas are selected (Yancheng Coastal wetland and Shijiu Lake), and the fully polarized SAR data based on Gaofen-3 are used to extract water bodies using the method of polarization decomposition and gray level co-occurrence matrix. Firstly, the multi-dimensional features of ground objects are extracted, and then the redundancy processing of multi-dimensional features is carried out by the separability index, which effectively solves the misclassification of non-water bodies and water bodies and improves the accuracy of water body extraction. The comparison between the results of full-polarization extraction and single-polarization extraction shows that both full-polarization and single-polarization extraction can extract water information, but the extraction accuracy of the full-polarization method can reach 94.74% in the area with complex wetland features, which can effectively compensate for the lack of precision of the single-polarization method. Although multi-dimensional features can be extracted from fully polarimetric SAR data, data redundancy may exist. Therefore, using the Separability index (SI) to process multi-dimensional features can effectively solve the problem of feature redundancy and improve classification accuracy.
Remediation of Benzene and 1,2-Dichloroethylene in Groundwater by Funnel and Gate Permeable Reactive Barrier (FGPRB): A Case Study
Authors: Gao Chunyang , Song Quanwei , Li Xingchun , Wang Ligang , Zhai Yong , Du Xianyuan , Yin Wei
Published: December 2021

Funnel and gate permeable reactive barrier (FGPRB) is an effective method to treat groundwater pollution. In order to clarify the impact of FGPRB on groundwater dynamic conditions, this study takes a site pilot test as the research object and establishes an FGPRB downstream of a petrochemical industry. The results show that the concentrations of 1,2-dichloroethylene and benzene in the downstream groundwater, after setting FGPRB, are lower than the detection limit. The numerical simulation results show that after setting FGPRB, both point source and area source pollution can achieve a good delay effect, extending from about 27 d to about 65 d of response time, but changing the thickness and permeability coefficient has no obvious effect on the delay effect. The tracer test shows the average permeability coefficient of the medium from the injection well to the monitoring well after the construction of FGPRB decreases from 77.0 m/d to 31.2 m/d after the construction of FGPRB. The average seepage velocity from the injection well to the monitoring well decreased from 0.19 m/d to 0.078 m/d after the construction of FGPRB. At the same time, when the FGPRB is not built, the maximum concentration time from the injection well to the monitoring well is about 10 d. After the FGPRB is constructed, the maximum concentration time of the tracer received by the monitoring well is about 27 days. These results confirm that the establishment of FGPRB will change the hydrodynamic conditions of groundwater and delay the response time of pollutants in the monitoring well.
Best Practices for Monitoring and Assessing the Ecological Response to River Restoration
Authors: England Judy , Angelopoulos Natalie , Cooksley Susan , Dodd Jennifer , Gill Andrew , Gilvear David , Johnson Matthew , Naura Marc , O’Hare Matthew , Tree Angus , Wheeldon Jennifer , Wilkes Martin A.
Published: December 2021

Nature-based solutions are widely advocated for freshwater ecosystem conservation and restoration. As increasing amounts of river restoration are undertaken, the need to understand the ecological response to different measures and where measures are best applied becomes more pressing. It is essential that appraisal methods follow a sound scientific approach. Here, experienced restoration appraisal experts review current best practice and academic knowledge to make recommendations and provide guidance that will enable practitioners to gather and analyse meaningful data, using scientific rigor to appraise restoration success. What should be monitored depends on the river type and the type and scale of intervention. By understanding how habitats are likely to change we can anticipate what species, life stages, and communities are likely to be affected. Monitoring should therefore be integrated and include both environmental/habitat and biota assessments. A robust scientific approach to monitoring and appraisal is resource intensive. We recommend that appraisal efforts be directed to where they will provide the greatest evidence, including ‘flagship’ restoration schemes for detailed long-term monitoring. Such an approach will provide the evidence needed to understand which restoration measures work where and ensure that they can be applied with confidence elsewhere.
Operating Effects of the Three Gorges Reservoir on the Riverbed Stability in the Wuhan Reach of the Yangtze River
Authors: Yin Zhi , Zhang Hongbin , Tan Guangming , Lyu Yiwei , Feng Zhiyong , Shu Caiwen , Wang Jingwen , Zhang Guangyue
Published: December 2021

Reservoir construction may modify the downstream flow and sediment transport, and correspondingly result in adjustments in morphodynamics of a river, especially riverbed instability. Based on hydrological datasets from 2003 to 2019 during the post-dam period using the topographic data of 57 fixed cross-sections in the Wuhan reach of the Yangtze River, we calculated the indexes representing the channel stability. Moreover, considering the effects of flow, sediment concentration, grain size of sediment, and water depth, we propose a method for calculating the equilibrium values of cumulative erosion and the lateral migrate intensity of thalweg in this paper, and the method combines with the delayed response model (DRM) to comprehensively analyze the variations in the longitudinal and lateral stabilities of the riverbed. The results revealed that the channel has been obviously eroded in the downstream reach, resulting in a 76% decrease in sediment discharge after the impoundment of the Three Gorges Reservoir (TGR). Specifically, in the past 17 years, the cumulative erosion in the Wuhan reach of the Yangtze River reached 1.72 m, while the bankfull depth increased by 1.87 m. The lateral migrate intensity of thalweg increased in response to Coriolis force, with an increase of 22.3%. Taken together, the results show that the proposed formula can effectively simulate the variation process of channel stability, and it also quantifies the extent of the influence weight of interannual flow and sediment regimes. The morphodynamics adjustments in the channel stability of our studied reach were closely related to the previous five-year flow and sediment regimes, implying that channel evolution may lag behind the changes in flow and sediment discharges.