bluuebunny/crossref_metadata_2025 huggingface dataset:
Work, Leisure Time Activities, and Mental Health among Family Caregivers of the Elder People in Japan
Authors: Omiya Tomoko , Kutsumi Masami , Fukui Sakiko
Published: January 2021

In Japan, there is a high incidence of family members caring for their elderly. To facilitate this, caregivers often quit their jobs, work reduced hours, and forfeit leisure activities. This study examined the relationship between the mental health of the caregivers and the sacrifices and adjustments they make to care for the elderly. A cross-sectional survey was conducted with responses from 171 caregivers. Referencing Pearlin’s stress process model, the relationship among five types of work change, four types of leisure activity quitting, caregivers’ subjective care burden, and depression were analyzed using t-test and multiple regression analysis. Caregivers who quit their work or other home activities had significantly more daily living care responsibilities than those who did not. Moreover, caregivers who gave up leisure activities had a greater sense of subjective care burden than those who did not. The experience of giving up peer activities and taking leave of absence from work was significantly associated with increased depressive symptoms. Being a part-timer or financially prosperous was associated with good mental health. To support family caregivers, it is essential to reduce the burden of long-term care and provide financial help and an environment where they interact with their peers, and their moods can be enhanced.
LACE Score-Based Risk Management Tool for Long-Term Home Care Patients: A Proof-of-Concept Study in Taiwan
Authors: Su Mei-Chin , Chen Yu-Chun , Huang Mei-Shu , Lin Yen-Hsi , Lin Li-Hwa , Chang Hsiao-Ting , Chen Tzeng-Ji
Published: January 2021

Background: Effectively predicting and reducing readmission in long-term home care (LTHC) is challenging. We proposed, validated, and evaluated a risk management tool that stratifies LTHC patients by LACE predictive score for readmission risk, which can further help home care providers intervene with individualized preventive plans. Method: A before-and-after study was conducted by a LTHC unit in Taiwan. Patients with acute hospitalization within 30 days after discharge in the unit were enrolled as two cohorts (Pre-Implement cohort in 2017 and Post-Implement cohort in 2019). LACE score performance was evaluated by calibration and discrimination (AUC, area under receiver operator characteristic (ROC) curve). The clinical utility was evaluated by negative predictive value (NPV). Results: There were 48 patients with 87 acute hospitalizations in Pre-Implement cohort, and 132 patients with 179 hospitalizations in Post-Implement cohort. These LTHC patients were of older age, mostly intubated, and had more comorbidities. There was a significant reduction in readmission rate by 44.7% (readmission rate 25.3% vs. 14.0% in both cohorts). Although LACE score predictive model still has room for improvement (AUC = 0.598), it showed the potential as a useful screening tool (NPV, 87.9%; 95% C.I., 74.2–94.8). The reduction effect is more pronounced in infection-related readmission. Conclusion: As real-world evidence, LACE score-based risk management tool significantly reduced readmission by 44.7% in this LTHC unit. Larger scale studies involving multiple homecare units are needed to assess the generalizability of this study.
Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids
Authors: Bertacchi Stefano , Pagliari Stefania , Cantù Chiara , Bruni Ilaria , Labra Massimo , Branduardi Paola
Published: January 2021

In the context of the global need to move towards circular economies, microbial cell factories can be employed thanks to their ability to use side-stream biomasses from the agro-industrial sector to obtain additional products. The valorization of residues allows for better and complete use of natural resources and, at the same time, for the avoidance of waste management to address our needs. In this work, we focused our attention on the microbial valorization of cinnamon waste material after polyphenol extraction (C-PEW) (Cinnamomum verum J.Presl), generally discarded without any additional processing. The sugars embedded in C-PEW were released by enzymatic hydrolysis, more compatible than acid hydrolysis with the subsequent microbial cultivation. We demonstrated that the yeast Rhodosporidium toruloides was able to grow and produce up to 2.00 (±0.23) mg/L of carotenoids in the resulting hydrolysate as a sole carbon and nitrogen source despite the presence of antimicrobial compounds typical of cinnamon. To further extend the potential of our finding, we tested other fungal cell factories for growth on the same media. Overall, these results are opening the possibility to develop separate hydrolysis and fermentation (SHF) bioprocesses based on C-PEW and microbial biotransformation to obtain high-value molecules.
Emergency Healthcare Providers’ Knowledge about and Attitudes toward Advance Directives: A Cross-Sectional Study between Nurses and Emergency Medical Technicians at an Emergency Department
Authors: Hong Sun Woo , Kim Shinmi , Yun Yu Jin , Jung Hyun Sook , Shim JaeLan , Kim JinShil
Published: January 2021

This study aimed to explore and compare knowledge levels about advance directives (ADs) and life-sustaining treatment (LST) plans in end-of-life patients between emergency nurses and emergency medical technicians (EMTs). Using a cross-sectional study design and convenience sampling, 96 nurses and 68 EMTs were recruited from 12 emergency medical centers. A survey on knowledge about and attitudes toward ADs was performed using both online and offline methods between November and December 2019. Emergency healthcare providers were conceptually knowledgeable regarding ADs and LST, although approximately half or fewer had knowledge about ADs (such as the legal process for preparation, family or healthcare providers’ role, and the healthcare proxy). The knowledge levels of nurses and EMTs were moderate. Nurses had significantly greater knowledge relative to EMTs about ADs and LST. Positive attitudes of emergency healthcare providers were also moderately low, with nurses having less positive views than EMTs. Significant differences regarding ADs were found, with younger emergency healthcare providers having fewer career years, no personal end-of-life experiences, and less need for ADs having less knowledge. Emergency healthcare providers’ knowledge about and attitudes toward ADs were moderately low, with EMTs demonstrating a greater knowledge deficit and nurses exhibiting lower positive attitudes. Younger and novice providers had lower knowledge, but younger providers had more positive attitudes, implying that professional education and training should begin early in their careers to enhance their confidence for emergency delivery of advanced care planning.
Academic Competence, Teacher–Student Relationship, and Violence and Victimisation in Adolescents: The Classroom Climate as a Mediator
Authors: Jiménez Teresa I. , Moreno-Ruiz David , Estévez Estefanía , Callejas-Jerónimo Juan Evaristo , López-Crespo Ginesa , Valdivia-Salas Sonsoles
Published: January 2021

School violence is a serious social and public health problem prevalent worldwide. Although the relevance of teacher and classroom factors is well established in the literature, few studies have focused on the role of teacher perceptions in school violence and victimisation and the potential mediational role of classroom climate in this relationship. A total of 2399 adolescents (50% girls), aged between 11 and 18 years (M = 14.65, SD = 1.78) and enrolled in five Spanish Secondary Compulsory Education schools completed measures of classroom climate, school violence towards peers and perception of peer victimisation, and their teachers informed about their academic competence and the teacher–student relationship. Correlational analyses revealed that whereas academic competence perceived by the teacher was negatively related to overt violence and victimisation, its relationship with pure relational violence was positive. Structural equation modelling analyses showed that variables of classroom climate (involvement, affiliation, and teacher support) perceived by the students functioned as partial mediators between teacher perceptions of academic competence and of teacher–student relationship and violence and victimisation. In the mediational model, teacher perception of academic competence acted as a direct protective factor against violence and victimisation, and teacher perception of teacher–student relationship acted as a direct risk for violence, as well as an indirect protective factor through classroom climate for victimisation. The interpretation of these results points to the importance of the teacher’s subjective perceptions in the prevention of violence and victimisation problems and their practical implications for the classroom climate perceived by students.
A Perception Model for Optimizing and Evaluating Evacuation Guidance Systems
Authors: Huang Lin , Gong Jianhua , Li Wenhang
Published: January 2021

To overcome the difficulties of quantitatively optimizing and evaluating evacuation guidance systems, we proposed a perception model based on virtual reality (VR) experiments and the social force model (SFM). We used VR and eye tracking devices to carry out experiments. The VR experiment data was mainly used for three purposes: to determine the parameter values of the perception model, to optimize the evacuation guidance system by quantitative analysis, and to validate the perception model. Additionally, we compared the VR experimental and model simulation results before and after the optimization to quantitatively assess the improvement in the optimized evacuation guidance system. The results showed that our model can effectively simulate the perception behaviors of evacuees on the evacuation guidance system and it can quantitatively evaluate different evacuation guidance system schemes. The model simulations showed that the optimized evacuation guidance system improved the evacuation efficiency, with the average escape time and distance of the two starting positions reduced by 37% and 28%, respectively.
The Cholinergic System, the Adrenergic System and the Neuropathology of Alzheimer’s Disease
Authors: Bekdash Rola A.
Published: January 2021

Neurodegenerative diseases are a major public health problem worldwide with a wide spectrum of symptoms and physiological effects. It has been long reported that the dysregulation of the cholinergic system and the adrenergic system are linked to the etiology of Alzheimer’s disease. Cholinergic neurons are widely distributed in brain regions that play a role in cognitive functions and normal cholinergic signaling related to learning and memory is dependent on acetylcholine. The Locus Coeruleus norepinephrine (LC-NE) is the main noradrenergic nucleus that projects and supplies norepinephrine to different brain regions. Norepinephrine has been shown to be neuroprotective against neurodegeneration and plays a role in behavior and cognition. Cholinergic and adrenergic signaling are dysregulated in Alzheimer’s disease. The degeneration of cholinergic neurons in nucleus basalis of Meynert in the basal forebrain and the degeneration of LC-NE neurons were reported in Alzheimer’s disease. The aim of this review is to describe current literature on the role of the cholinergic system and the adrenergic system (LC-NE) in the pathology of Alzheimer’s disease and potential therapeutic implications.
Convergence and Divergence of Sugar and Cytokinin Signaling in Plant Development
Authors: Wang Ming , Le Gourrierec José , Jiao Fuchao , Demotes-Mainard Sabine , Perez-Garcia Maria-Dolores , Ogé Laurent , Hamama Latifa , Crespel Laurent , Bertheloot Jessica , Chen Jingtang , Grappin Philippe , Sakr Soulaiman
Published: January 2021

Plants adjust their growth and development through a sophisticated regulatory system integrating endogenous and exogenous cues. Many of them rely on intricate crosstalk between nutrients and hormones, an effective way of coupling nutritional and developmental information and ensuring plant survival. Sugars in their different forms such as sucrose, glucose, fructose and trehalose-6-P and the hormone family of cytokinins (CKs) are major regulators of the shoot and root functioning throughout the plant life cycle. While their individual roles have been extensively investigated, their combined effects have unexpectedly received little attention, resulting in many gaps in current knowledge. The present review provides an overview of the relationship between sugars and CKs signaling in the main developmental transition during the plant lifecycle, including seed development, germination, seedling establishment, root and shoot branching, leaf senescence, and flowering. These new insights highlight the diversity and the complexity of the crosstalk between sugars and CKs and raise several questions that will open onto further investigations of these regulation networks orchestrating plant growth and development.
Neurochemical Effects of 4-(2Chloro-4-Fluorobenzyl)-3-(2-Thienyl)-1,2,4-Oxadiazol-5(4H)-One in the Pentylenetetrazole (PTZ)-Induced Epileptic Seizure Zebrafish Model
Authors: Kim Seong Soon , Kan Hyemin , Hwang Kyu-Seok , Yang Jung Yoon , Son Yuji , Shin Dae-Seop , Lee Byung Hoi , Ahn Se Hwan , Ahn Jin Hee , Cho Sung-Hee , Bae Myung Ae
Published: January 2021

Epilepsy is one of the most common neurological disorders, and it is characterized by spontaneous seizures. In a previous study, we identified 4-(2-chloro-4-fluorobenzyl)-3-(2-thienyl)-1,2,4-oxadiazol-5(4H)-one (GM-90432) as a novel anti-epileptic agent in chemically- or genetically-induced epileptic zebrafish and mouse models. In this study, we investigated the anti-epileptic effects of GM-90432 through neurochemical profiling-based approach to understand the neuroprotective mechanism in a pentylenetetrazole (PTZ)-induced epileptic seizure zebrafish model. GM-90432 effectively improved PTZ-induced epileptic behaviors via upregulation of 5-hydroxytryptamine, 17-β-estradiol, dihydrotestosterone, progesterone, 5α -dihydroprogesterone, and allopregnanolone levels, and downregulation of normetanephrine, gamma-aminobutyric acid, and cortisol levels in brain tissue. GM-90432 also had a protective effect against PTZ-induced oxidative stress and zebrafish death, suggesting that it exhibits biphasic neuroprotective effects via scavenging of reactive oxygen species and anti-epileptic activities in a zebrafish model. In conclusion, our results suggest that neurochemical profiling study could be used to better understand of anti-epileptic mechanism of GM-90432, potentially leading to new drug discovery and development of anti-seizure agents.
Known Drugs Identified by Structure-Based Virtual Screening Are Able to Bind Sigma-1 Receptor and Increase Growth of Huntington Disease Patient-Derived Cells
Authors: Battista Theo , Pascarella Gianmarco , Staid David Sasah , Colotti Gianni , Rosati Jessica , Fiorillo Annarita , Casamassa Alessia , Vescovi Angelo Luigi , Giabbai Barbara , Semrau Marta Stefania , Fanelli Sergio , Storici Paola , Squitieri Ferdinando , Morea Veronica , Ilari Andrea
Published: January 2021

Huntington disease (HD) is a devastating and presently untreatable neurodegenerative disease characterized by progressively disabling motor and mental manifestations. The sigma-1 receptor (σ1R) is a protein expressed in the central nervous system, whose 3D structure has been recently determined by X-ray crystallography and whose agonists have been shown to have neuroprotective activity in neurodegenerative diseases. To identify therapeutic agents against HD, we have implemented a drug repositioning strategy consisting of: (i) Prediction of the ability of the FDA-approved drugs publicly available through the ZINC database to interact with σ1R by virtual screening, followed by computational docking and visual examination of the 20 highest scoring drugs; and (ii) Assessment of the ability of the six drugs selected by computational analyses to directly bind purified σ1R in vitro by Surface Plasmon Resonance and improve the growth of fibroblasts obtained from HD patients, which is significantly impaired with respect to control cells. All six of the selected drugs proved able to directly bind purified σ1R in vitro and improve the growth of HD cells from both or one HD patient. These results support the validity of the drug repositioning procedure implemented herein for the identification of new therapeutic tools against HD.
Auxin-Induced SaARF4 Downregulates SaACO4 to Inhibit Lateral Root Formation in Sedum alfredii Hance
Authors: Xu Dong , Lu Zhuchou , Qiao Guirong , Qiu Wenmin , Wu Longhua , Han Xiaojiao , Zhuo Renying
Published: January 2021

Lateral root (LR) formation promotes plant resistance, whereas high-level ethylene induced by abiotic stress will inhibit LR emergence. Considering that local auxin accumulation is a precondition for LR generation, auxin-induced genes inhibiting ethylene synthesis may thus be important for LR development. Here, we found that auxin response factor 4 (SaARF4) in Sedum alfredii Hance could be induced by auxin. The overexpression of SaARF4 decreased the LR number and reduced the vessel diameters. Meanwhile, the auxin distribution mode was altered in the root tips and PIN expression was also decreased in the overexpressed lines compared with the wild-type (WT) plants. The overexpression of SaARF4 could reduce ethylene synthesis, and thus, the repression of ethylene production decreased the LR number of WT and reduced PIN expression in the roots. Furthermore, the quantitative real-time PCR, chromatin immunoprecipitation sequencing, yeast one-hybrid, and dual-luciferase assay results showed that SaARF4 could bind the promoter of 1-aminocyclopropane-1-carboxylate oxidase 4 (SaACO4), associated with ethylene biosynthesis, and could downregulate its expression. Therefore, we concluded that SaARF4 induced by auxin can inhibit ethylene biosynthesis by repressing SaACO4 expression, and this process may affect auxin transport to delay LR development.
The SARS-Coronavirus Infection Cycle: A Survey of Viral Membrane Proteins, Their Functional Interactions and Pathogenesis
Authors: Wong Nicholas A. , Saier Milton H.
Published: January 2021

Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is a novel epidemic strain of Betacoronavirus that is responsible for the current viral pandemic, coronavirus disease 2019 (COVID-19), a global health crisis. Other epidemic Betacoronaviruses include the 2003 SARS-CoV-1 and the 2009 Middle East Respiratory Syndrome Coronavirus (MERS-CoV), the genomes of which, particularly that of SARS-CoV-1, are similar to that of the 2019 SARS-CoV-2. In this extensive review, we document the most recent information on Coronavirus proteins, with emphasis on the membrane proteins in the Coronaviridae family. We include information on their structures, functions, and participation in pathogenesis. While the shared proteins among the different coronaviruses may vary in structure and function, they all seem to be multifunctional, a common theme interconnecting these viruses. Many transmembrane proteins encoded within the SARS-CoV-2 genome play important roles in the infection cycle while others have functions yet to be understood. We compare the various structural and nonstructural proteins within the Coronaviridae family to elucidate potential overlaps and parallels in function, focusing primarily on the transmembrane proteins and their influences on host membrane arrangements, secretory pathways, cellular growth inhibition, cell death and immune responses during the viral replication cycle. We also offer bioinformatic analyses of potential viroporin activities of the membrane proteins and their sequence similarities to the Envelope (E) protein. In the last major part of the review, we discuss complement, stimulation of inflammation, and immune evasion/suppression that leads to CoV-derived severe disease and mortality. The overall pathogenesis and disease progression of CoVs is put into perspective by indicating several stages in the resulting infection process in which both host and antiviral therapies could be targeted to block the viral cycle. Lastly, we discuss the development of adaptive immunity against various structural proteins, indicating specific vulnerable regions in the proteins. We discuss current CoV vaccine development approaches with purified proteins, attenuated viruses and DNA vaccines.
Effect of O-Antigen Chain Length Regulation on the Immunogenicity of Shigella and Salmonella Generalized Modules for Membrane Antigens (GMMA)
Authors: Gasperini Gianmarco , Raso Maria Michelina , Arato Vanessa , Aruta Maria Grazia , Cescutti Paola , Necchi Francesca , Micoli Francesca
Published: January 2021

Recently, generalized modules for membrane antigens (GMMA) technology has been proposed as an alternative approach to traditional glycoconjugate vaccines for O-antigen delivery. Saccharide length is a well-known parameter that can impact the immune response induced by glycoconjugates both in terms of magnitude and quality. However, the criticality of O-antigen length on the immune response induced by GMMA-based vaccines has not been fully elucidated. Here, Shigella and Salmonella GMMA-producing strains were further mutated in order to display homogeneous polysaccharide populations of different sizes on a GMMA surface. Resulting GMMA were compared in mice immunization studies. Athymic nude mice were also used to investigate the involvement of T-cells in the immune response elicited. In contrast with what has been reported for traditional glycoconjugate vaccines and independent of the pathogen and the sugar structural characteristics, O-antigen length did not result in being a critical parameter for GMMA immunogenicity. This work supports the identification of critical quality attributes to optimize GMMA vaccine design and improve vaccine efficacy and gives insights on the nature of the immune response induced by GMMA.
Identification of New Genes Involved in Germline Predisposition to Early-Onset Gastric Cancer
Authors: Herrera-Pariente Cristina , Capó-García Roser , Díaz-Gay Marcos , Carballal Sabela , Muñoz Jenifer , Llach Joan , Sánchez Ariadna , Bonjoch Laia , Arnau-Collell Coral , Soares de Lima Yasmin , Golubicki Mariano , Jung Gerhard , Lozano Juan José , Castells Antoni , Balaguer Francesc , Bujanda Luis , Castellví-Bel Sergi , Moreira Leticia
Published: January 2021

The genetic cause for several families with gastric cancer (GC) aggregation is unclear, with marked relevance in early-onset patients. We aimed to identify new candidate genes involved in GC germline predisposition. Whole-exome sequencing (WES) of germline samples was performed in 20 early-onset GC patients without previous germline mutation identified. WES was also performed in nine tumor samples to analyze the somatic profile using SigProfilerExtractor tool. Sequencing germline data were filtered to select those variants with plausible pathogenicity, rare frequency and previously involved in cancer. Then, a manual filtering was performed to prioritize genes according to current knowledge and function. These genetic variants were prevalidated with Integrative Genomics Viewer 2.8.2 (IGV). Subsequently, a further selection step was carried out according to function and information obtained from tumor samples. After IGV and selection step, 58 genetic variants in 52 different candidate genes were validated by Sanger sequencing. Among them, APC, FAT4, CTNND1 and TLR2 seem to be the most promising genes because of their role in hereditary cancer syndromes, tumor suppression, cell adhesion and Helicobacter pylori recognition, respectively. These encouraging results represent the open door to the identification of new genes involved in GC germline predisposition.
Gut Microbial Signatures in Sporadic and Hereditary Colorectal Cancer
Authors: Mori Giorgia , Pasca Maria Rosalia
Published: January 2021

Colorectal cancer (CRC) is the fourth most common cause of cancer-related death and the third most common cancer in the world. Depending on the origin of the mutation, colorectal carcinomas are classified as sporadic or hereditary. Cancers derived from mutations appearing during life, affecting individual cells and their descendants, are called sporadic and account for almost 95% of the CRCs. Less than 5% of CRC cases result from constitutional mutations conferring a very high risk of developing cancer. Screening for hereditary-related cancers is offered to individuals at risk for hereditary CRC, who have either not undergone genetic evaluation or have uncertain genetic test results. In this review, we briefly summarize the main findings on the correlation between sporadic CRC and the gut microbiota, and we specifically focus on the few evidences about the role that gut microorganisms have on the development of CRC hereditary syndromes. The characterization of a gut microbiota associated with an increased risk of developing CRC could have a profound impact for prevention purposes. We also discuss the potential role of the gut microbiota as therapeutic treatment.
DAXX Is a Crucial Factor for Proper Development of Mammalian Oocytes and Early Embryos
Authors: Bogolyubova Irina , Bogolyubov Dmitry
Published: January 2021

The Death-domain associated protein 6 (DAXX) is an evolutionarily conserved and ubiquitously expressed multifunctional protein that is implicated in many cellular processes, including transcription, cellular proliferation, cell cycle regulation, Fas-induced apoptosis, and many other events. In the nucleus, DAXX interacts with transcription factors, epigenetic modifiers, and chromatin-remodeling proteins such as the transcription regulator ATRX—the α-thalassemia/mental retardation syndrome X-linked ATP-dependent helicase II. Accordingly, DAXX is considered one of the main players involved in chromatin silencing and one of the most important factors that maintain integrity of the genome. In this brief review, we summarize available data regarding the general and specific functions of DAXX in mammalian early development, with special emphasis on the function of DAXX as a chaperone of the histone variant H3.3. Since H3.3 plays a key role in the developmental processes, especially in the pronounced rearrangements of heterochromatin compartment during oogenesis and embryogenesis, DAXX can be considered as an important factor supporting proper development. Specifically, loss of DAXX affects the recruitment of ATRX, transcription of tandem repeats and telomere functions, which results in a decrease in the viability of early embryos.
Congenital Diarrhea and Cholestatic Liver Disease: Phenotypic Spectrum Associated with MYO5B Mutations
Authors: Aldrian Denise , Vogel Georg F. , Frey Teresa K. , Ayyıldız Civan Hasret , Aksu Aysel Ünlüsoy , Avitzur Yaron , Ramos Boluda Esther , Çakır Murat , Demir Arzu Meltem , Deppisch Caroline , Duba Hans-Christoph , Düker Gesche , Gerner Patrick , Hertecant Jozef , Hornová Jarmila , Kathemann Simone , Koeglmeier Jutta , Koutroumpa Arsinoi , Lanzersdorfer Roland , Lev-Tzion Raffi , Lima Rosa , Mansour Sahar , Meissl Manfred , Melek Jan , Miqdady Mohamad , Montoya Jorge Hernan , Posovszky Carsten , Rachman Yelena , Siahanidou Tania , Tabbers Merit , Uhlig Holm H. , Ünal Sevim , Wirth Stefan , Ruemmele Frank M. , Hess Michael W. , Huber Lukas A. , Müller Thomas , Sturm Ekkehard , Janecke Andreas R.
Published: January 2021

Myosin Vb (MYO5B) is a motor protein that facilitates protein trafficking and recycling in polarized cells by RAB11- and RAB8-dependent mechanisms. Biallelic MYO5B mutations are identified in the majority of patients with microvillus inclusion disease (MVID). MVID is an intractable diarrhea of infantile onset with characteristic histopathologic findings that requires life-long parenteral nutrition or intestinal transplantation. A large number of such patients eventually develop cholestatic liver disease. Bi-allelic MYO5B mutations are also identified in a subset of patients with predominant early-onset cholestatic liver disease. We present here the compilation of 114 patients with disease-causing MYO5B genotypes, including 44 novel patients as well as 35 novel MYO5B mutations, and an analysis of MYO5B mutations with regard to functional consequences. Our data support the concept that (1) a complete lack of MYO5B protein or early MYO5B truncation causes predominant intestinal disease (MYO5B-MVID), (2) the expression of full-length mutant MYO5B proteins with residual function causes predominant cholestatic liver disease (MYO5B-PFIC), and (3) the expression of mutant MYO5B proteins without residual function causes both intestinal and hepatic disease (MYO5B-MIXED). Genotype-phenotype data are deposited in the existing open MYO5B database in order to improve disease diagnosis, prognosis, and genetic counseling.
The Potential Use of Radiomics with Pre-Radiation Therapy MR Imaging in Predicting Risk of Pseudoprogression in Glioblastoma Patients
Authors: Baine Michael , Burr Justin , Du Qian , Zhang Chi , Liang Xiaoying , Krajewski Luke , Zima Laura , Rux Gerard , Zhang Chi , Zheng Dandan
Published: January 2021

Glioblastoma (GBM) is the most common adult glioma. Differentiating post-treatment effects such as pseudoprogression from true progression is paramount for treatment. Radiomics has been shown to predict overall survival and MGMT (methylguanine-DNA methyltransferase) promoter status in those with GBM. A potential application of radiomics is predicting pseudoprogression on pre-radiotherapy (RT) scans for patients with GBM. A retrospective review was performed with radiomic data analyzed using pre-RT MRI scans. Pseudoprogression was defined as post-treatment findings on imaging that resolved with steroids or spontaneously on subsequent imaging. Of the 72 patients identified for the study, 35 were able to be assessed for pseudoprogression, and 8 (22.9%) had pseudoprogression. A total of 841 radiomic features were examined along with clinical features. Receiver operating characteristic (ROC) analyses were performed to determine the AUC (area under ROC curve) of models of clinical features, radiomic features, and combining clinical and radiomic features. Two radiomic features were identified to be the optimal model combination. The ROC analysis found that the predictive ability of this combination was higher than using clinical features alone (mean AUC: 0.82 vs. 0.62). Additionally, combining the radiomic features with clinical factors did not improve predictive ability. Our results indicate that radiomics is potentially capable of predicting future development of pseudoprogression in patients with GBM using pre-RT MRIs.
Acknowledgment to Reviewers of JMMP in 2020
Authors:   JMMP Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that JMMP maintains its standards for the high quality of its published papers [...]
Acid pH Strategy Adaptation through NRG1 in Ustilago maydis
Authors: Sánchez-Arreguin José Alejandro , Ruiz-Herrera José , Mares-Rodriguez F. de Jesus , León-Ramírez Claudia Geraldine , Sánchez-Segura Lino , Zapata-Morín Patricio Adrián , Coronado-Gallegos Jordan , Aréchiga-Carvajal Elva Teresa
Published: January 2021

The role of the Ustilago maydis putative homolog of the transcriptional repressor ScNRG1, previously described in Saccharomyces cerevisiae, Candida albicans and Cryptococcus neoformans, was analyzed by means of its mutation. In S. cerevisiae this gene regulates a set of stress-responsive genes, and in C. neoformans it is involved in pathogenesis. It was observed that the U. maydisNRG1 gene regulates several aspects of the cell response to acid pH, such as the production of mannosyl-erythritol lipids, inhibition of the expression of the siderophore cluster genes, filamentous growth, virulence and oxidative stress. A comparison of the gene expression pattern of the wild type strain versus the nrg1 mutant strain of the fungus, through RNA Seq analyses, showed that this transcriptional factor alters the expression of 368 genes when growing at acid pH (205 up-regulated, 163 down-regulated). The most relevant genes affected by NRG1 were those previously reported as the key ones for particular cellular stress responses, such as HOG1 for osmotic stress and RIM101 for alkaline pH. Four of the seven genes included WCO1 codifying PAS domain ( These has been shown as the key structural motif involved in protein-protein interactions of the circadian clock, and it is also a common motif found in signaling proteins, where it functions as a signaling sensor) domains sensors of blue light, two of the three previously reported to encode opsins, one vacuolar and non-pH-responsive, and another one whose role in the acid pH response was already known. It appears that all these light-reactive cell components are possibly involved in membrane potential equilibrium and as virulence sensors. Among previously described specific functions of this transcriptional regulator, it was found to be involved in glucose repression, metabolic adaptation to adverse conditions, cellular transport, cell rescue, defense and interaction with an acidic pH environment.
Self-Generated Hypoxia Leads to Oxidative Stress and Massive Death in Ustilago maydis Populations under Extreme Starvation and Oxygen-Limited Conditions
Authors: Petkovic Jelena , Kojic Milorad , Milisavljevic Mira
Published: January 2021

Ustilago maydis and Saccharomyces cerevisiae differ considerably in their response to water-transfer treatments. When stationary phase cells were transferred to pure water and incubated under limited supply of oxygen, the U. maydis cells suffered a catastrophic loss of viability while the S. cerevisiae population was virtually unaffected by the treatment. The major factor underlying the death of the U. maydis cells under those conditions was an oxygen-consuming cellular activity that generated a hypoxic environment, thereby inducing oxidative stress and accumulation of reactive oxygen species, which resulted in lethality. Importantly, a small residue of U. maydis cells that did survive was able to resume growth and repopulate up to the initial culture density when sufficient aeration was restored. The regrowth was dependent on the cellular factors (Adr1, Did4, Kel1, and Tbp1), previously identified as required for repopulation, after killing with hydrogen peroxide. Surprisingly, the survivors were also able to resume growth under apparently hypoxic conditions, indicating that these remnant cells likely switched to a fermentative mode of growth. We discuss the findings in terms of their possible relevance to the eco-evolutionary adaptation of U. maydis to risky environments.
Dialoguing with Data and Data Reduction: An Observational, Narrowing-Down Approach to Social Media Network Analysis
Authors: Tong Jingrong , Zuo Landong
Published: January 2021

In this article, we propose an observational, narrowing-down approach to analysing social media networks and developing research design by the joint use of computational algorithms and researchers’ inductive exploration and interpretive explanations. The Brexit referendum on Twitter study is used to illustrate how we applied this approach in practice. In this study, observation helped us combine the strengths of computational statistical analysis and modelling and of inductive inquiries. Computational algorithms and tools including Elasticsearch, Kibana and Gephi provided us with an “ethnographic field” where we were able to inductively observe the relationships among users and to reduce the amount of data down to a level in which we could intuitively understand these relationships. In traditional observational studies, talking to human subjects and observing their interactions in a research site are important to ethnographers. Likewise, it is useful for social science researchers to dialogue with data, observe human relationships embodied in the data and reconstructed by computational tools, and understand these relationships through closely examining a small batch of meaningful data that is extracted from large-scale data. In this case study, adopting the proposed approach, we found the importance of political disagreement leading to a tale of two politicians, in which pro-Brexit users denounced @David_Cameron but legitimised @Nigel_Farage.
Improved Smart Pillow for Remote Health Care System
Authors: Li Songsheng , Chiu Christopher
Published: January 2021

The outbreak of novel coronavirus (COVID-19) resulted in the clinical decision that reduced direct contact is optimal, especially for senior citizens residing in nursing homes. A smart pillow adapted for the Remote Healthcare System is presented in this paper, whose core is a Bluetooth (BT) host equipped with temperature and pressure sensors. The measurement of Core Body Temperature (CBT) from the perspective of heat transfer is first analyzed, with two proven effective methods introduced—classical Zero-Heat-Flux (ZHF) and Dual-Heat-Flux (DHF)—then finally the similarities between the Smart Pillow and ZHF are demonstrated. A pressure pad is inserted inside the pillow to detect occupancy and the specific position of the head on the pillow that meets clinical diagnostic needs. Furthermore, a real-time proactive monitoring mode is enabled for urgent warnings, which forces the pillow to keep detecting and reporting data in a defined time duration but results in rapid battery drain of the pillow. In this way, the system can detect the CBT and in-bed situation of the inhabitant without being physically present to determine critical measurements. Utility of this system can be extended to elderly people living alone in regional or remote areas, such that medical help can be dispatched as soon as possible in case of medical emergency.
A New Measure of Complementarity in Market Basket Data
Authors: Puka Radosław , Jedrusik Stanislaw
Published: January 2021

Modern IT systems collect detailed data on each activity, transaction, forum entry, conversation and many other areas. The availability of large data volumes in the business, industry and research fields opens up new opportunities for the empirical verification of various economic theories and laws. The analysis of big datasets in turn allows us to look at many issues from a new point of view and see the dependencies that are otherwise difficult to derive. In this paper, we propose a new measure for dependencies between goods in market basket data. The introduced measure was inspired by the well-known microeconomic concept of complementarity. Due to its similar properties to those of complementarity, the new measure was called basket complementarity (b-complementarity). B-complementarity not only measures the strength of dependencies between goods but also measures the direction of these dependencies. The values of the proposed measure can be relatively easily calculated using market basket data. This paper also presents a simple example illustrating this new concept, areas of possible application (e.g., in e-commerce) and preliminary results of searching for goods that meet the criteria of basket complementarity in real market basket data.
A Game Analysis of Farmland Expropriation Conflict in China under Multi-Dimensional Preference: Cooperation or Resistance?
Authors: Hong Kairong , Zou Yucheng , Zhu Mingyuan , Zhang Yanwei
Published: January 2021

The process of urbanization in China has been accompanied by the conflict of land expropriation, which is not conducive to social stability. Different from the previous angles and methods of studying the conflict of agricultural land expropriation, this study puts forward a new behavioral perspective on the basis of game theory, and constructs an evolutionary game model of the conflict of agricultural land expropriation in China from the perspective of multi-dimensional preference. It also discusses the impact of different preference combinations on the conflict input, net income and utility of various stakeholders in agricultural land expropriation. The results show that under the influence of complete self-interest preference, the degree of protection of farmers’ land rights and interests affects the probability of conflicts in compensation for agricultural land expropriation. However, under the influence of multi-dimensional preference combination, agricultural land expropriation can be carried out smoothly only when the reciprocal altruism preference of local government and farmers is strong and the loss aversion preference is weak. These insights have implications for the sustainable development of land, including government involvement and farmers participation, particularly in the context of developing countries.
Combining Tree Species Composition and Understory Coverage Indicators with Optimization Techniques to Address Concerns with Landscape-Level Biodiversity
Authors: Botequim Brigite , Bugalho Miguel N. , Rodrigues Ana Raquel , Marques Susete , Marto Marco , Borges José G.
Published: January 2021

Sustainable forest management needs to address biodiversity conservation concerns. For that purpose, forest managers need models and indicators that may help evaluate the impact of management options on biodiversity under the uncertainty of climate change scenarios. In this research we explore the potential for designing mosaics of stand-level forest management models to address biodiversity conservation objectives on a broader landscape-level. Our approach integrates (i) an effective stand-level biodiversity indicator that reflect tree species composition, stand age, and understory coverage under divergent climate conditions; and (ii) linear programming optimization techniques to guide forest actors in seeing optimal forest practices to safeguard future biodiversity. Emphasis is on the efficiency and effectiveness of an approach to help assess the impact of forest management planning on biodiversity under scenarios of climate change. Results from a resource capability model are discussed for an application to a large-scale problem encompassing 14,765 ha, extending over a 90-years planning horizon and considering two local-climate scenarios. They highlight the potential of the approach to help assess the impact of both stand and landscape-level forest management models on biodiversity conservation goals. They demonstrate further that the approach provides insights about how climate change, timber demand and wildfire resistance may impact plans that target the optimization of biodiversity values. The set of optimized long-term solutions emphasizes a multifunctional forest that guarantees a desirable local level of biodiversity and resilience to wildfires, while providing a balanced production of wood over time at the landscape scale.
Novel Selenoureas Based on Cinchona Alkaloid Skeleton: Synthesis and Catalytic Investigations
Authors: Zielińska-Błajet Mariola , Najdek Joanna
Published: January 2021

An efficient approach to the synthesis of chiral selenoureas consisting of Cinchona alkaloid scaffolds was described. The new selenoureas were assessed as bifunctional organocatalysts in the asymmetric Michael addition reactions under mild conditions. The best results were obtained for selenoureas bearing the 4-fluorophenyl group. These catalysts promoted the reactions with enantioselectivities of up to 96% ee. Additionally, the catalytic performance of the thiourea and selenourea counterpart was compared.
Influence of High Temperature on the Fracture Properties of Polyolefin Fibre Reinforced Concrete
Authors: Alberti Marcos García , Gálvez Jaime Carlos , Enfedaque Alejandro , Castellanos Ramiro
Published: January 2021

Concrete has become the most common construction material, showing, among other advantages, good behaviour when subjected to high temperatures. Nevertheless, concrete is usually reinforced with elements of other materials such as steel in the form of rebars or fibres. Thus, the behaviour under high temperatures of these other materials can be critical for structural elements. In addition, concrete spalling occurs when concrete is subjected to high temperature due to internal pressures. Micro polypropylene fibres (PP) have shown to be effective for reducing such spalling, although this type of fibres barely improves any of the mechanical properties of the element. Hence, a combination of PP with steel rebars or fibres can be effective for the structural design of elements exposed to high temperatures. New polyolefin fibres (PF) have become an alternative to steel fibres. PF meet the requirements of the standards to consider the contributions of the fibres in the structural design. However, there is a lack of evidence about the behaviour of PF and elements made of polyolefin fibre reinforced concrete (PFRC) subjected to high temperatures. Given that these polymer fibres would be melt above 250 °C, the behaviour in the intermediate temperatures was assessed in this study. Uni-axial tests on individual fibres and three-point bending tests of PFRC specimens were performed. The results have shown that the residual load-bearing capacity of the material is gradually lost up to 200 °C, though the PFRC showed structural performance up to 185 °C.
Copper Nitride Nanowire Arrays—Comparison of Synthetic Approaches
Authors: Scigala Aleksandra , Szłyk Edward , Rerek Tomasz , Wiśniewski Marek , Skowronski Lukasz , Trzcinski Marek , Szczesny Robert
Published: January 2021

Copper nitride nanowire arrays were synthesized by an ammonolysis reaction of copper oxide precursors grown on copper surfaces in an ammonia solution. The starting Cu films were deposited on a silicon substrate using two different methods: thermal evaporation (30 nm thickness) and electroplating (2 μm thickness). The grown CuO or CuO/Cu(OH)2 architectures were studied in regard to morphology and size, using electron microscopy methods (SEM, TEM). The final shape and composition of the structures were mostly affected by the concentration of the ammonia solution and time of the immersion. Needle-shaped 2–3 μm long nanostructures were formed from the electrodeposited copper films placed in a 0.033 M NH3 solution for 48 h, whereas for the copper films obtained by physical vapor deposition (PVD), well-aligned nano-needles were obtained after 3 h. The phase composition of the films was studied by X-ray diffraction (XRD) and selected area electron diffraction (SAED) analysis, indicating a presence of CuO and Cu(OH)2, as well as Cu residues. Therefore, in order to obtain a pure oxide film, the samples were thermally treated at 120–180 °C, after which the morphology of the structures remained unchanged. In the final stage of this study, Cu3N nanostructures were obtained by an ammonolysis reaction at 310 °C and studied by SEM, TEM, XRD, and spectroscopic methods. The fabricated PVD-derived coatings were also analyzed using a spectroscopic ellipsometry method, in order to calculate dielectric function, band gap and film thickness.