bluuebunny/crossref_metadata_2025 huggingface dataset:
Progress in the Development of Electrodeposited Catalysts for Direct Liquid Fuel Cell Applications
Authors: Maniam Kranthi Kumar , Chetty Raghuram , Thimmappa Ravikumar , Paul Shiladitya
Published: January 2022

Fuel cells are a key enabling technology for the future economy, thereby providing power to portable, stationary, and transportation applications, which can be considered an important contributor towards reducing the high dependencies on fossil fuels. Electrocatalyst plays a vital role in improving the performance of the low temperature fuel cells. Noble metals (Pt, Pd) supported on carbon have shown promising performance owing to their high catalytic activity for both electroreduction and electrooxidation and have good stability. Catalyst preparation by electrodeposition is considered to be simple in terms of operation and scalability with relatively low cost to obtain high purity metal deposits. This review emphasises the role of electrodeposition as a cost-effective method for synthesising fuel cell catalysts, summarising the progress in the electrodeposited Pt and Pd catalysts for direct liquid fuel cells (DLFCs). Moreover, this review also discusses the technological advances made utilising these catalysts in the past three decades, and the factors that impede the technological advancement of the electrodeposition process are presented. The challenges and the fundamental research strategies needed to achieve the commercial potential of electrodeposition as an economical, efficient methodology for synthesising fuel cells catalysts are outlined with the necessary raw materials considering current and future savings scenario.
Effects of Minimalist Footwear and Foot Strike Pattern on Plantar Pressure during a Prolonged Running
Authors: Izquierdo-Renau Marta , Sanchis-Sanchis Roberto , Priego-Quesada Jose I. , Encarnación-Martínez Alberto , Queralt Ana , Pérez-Soriano Pedro
Published: January 2022

The use of minimalist shoes (MS) in running involves changes in running mechanics compared to conventional shoes (CS), but there is still little research analysing the effects of this footwear on plantar pressure, which could help to understand some risk injury factors. Moreover, there are no studies examining the effects of a prolonged running and foot strike patterns on baropodometric variables in MS. Therefore, the aim of this study was to analyse the changes produced using MS on plantar pressure during a prolonged running, as well as its interaction with the time and foot strike pattern. Twenty-one experienced minimalist runners (age 38 ± 10 years, MS running experience 2 ± 1 years) ran with MS and CS for 30 min at 80% of their maximal aerobic speed, and mean pressure, peak pressure, contact time, centre of pressure velocity, relative force and contact area were analysed using a pressure platform. Foot strike pattern and time were also considered as factors. The multivariable linear regression mixed models showed that the use of MS induced, at the end of a prolonged running, higher peak pressure (p = 0.008), lower contact time (p = 0.004) and lower contact area (p < 0.001) than using CS. Also, runners with forefoot strike pattern using MS, compared to midfoot and rearfoot patterns, showed higher mean and peak pressure (p < 0.001) and lower contact time and area (p < 0.05). These results should be considered when planning training for runners using MS, as higher peak pressure values when using this type of footwear could be a risk factor for the development of some foot injuries.
Mix Design and Engineering Properties of Fiber-Reinforced Pervious Concrete Using Lightweight Aggregates
Authors: Tang Chao-Wei , Cheng Chiu-Kuei , Ean Lee-Woen
Published: January 2022

The main purpose of this study was to investigate the mix design and performance of fiber-reinforced pervious concrete using lightweight coarse aggregates instead of ordinary coarse aggregates. There were two main stages in the relevant testing work. First, the properties of the matrix were tested with a rheological test and then different amounts of lightweight coarse aggregate and fine aggregate were added to the matrix to measure the properties of the obtained lightweight pervious concrete (LPC). In order to greatly reduce the experimental workload, the Taguchi experimental design method was adopted. An orthogonal array L9(34) was used, which was composed of four controllable three-level factors. There were four test parameters in this study, which were the lightweight coarse aggregate size, ordinary fine aggregate content, matrix type, and aggregate/binder ratio. The research results confirmed that the use of suitable materials and the optimal mix proportions were the key factors for improving the mechanical properties of the LPC. Due to the use of silica fume, ultrafine silica powder, and polypropylene fibers, the 28-day compressive strength, 28-day flexural strength, and 28-day split tensile strength of the LPC specimens prepared in this study were 4.80–7.78, 1.19–1.86, and 0.78–1.11 MPa, respectively. On the whole, the mechanical properties of the prepared LPC specimens were better than those of the LPC with general composition.
Deep Learning Based Inversion of Locally Anisotropic Weld Properties from Ultrasonic Array Data
Authors: Singh Jonathan , Tant Katherine , Mulholland Anthony , MacLeod Charles
Published: January 2022

The ability to reliably detect and characterise defects embedded in austenitic steel welds depends on prior knowledge of microstructural descriptors, such as the orientations of the weld’s locally anisotropic grain structure. These orientations are usually unknown but it has been shown recently that they can be estimated from ultrasonic scattered wave data. However, conventional algorithms used for solving this inverse problem incur a significant computational cost. In this paper, we propose a framework which uses deep neural networks (DNNs) to reconstruct crystallographic orientations in a welded material from ultrasonic travel time data, in real-time. Acquiring the large amount of training data required for DNNs experimentally is practically infeasible for this problem, therefore a model based training approach is investigated instead, where a simple and efficient analytical method for modelling ultrasonic wave travel times through given weld geometries is implemented. The proposed method is validated by testing the trained networks on data arising from sophisticated finite element simulations of wave propagation through weld microstructures. The trained deep neural network predicts grain orientations to within 3° and in near real-time (0.04 s), presenting a significant step towards realising real-time, accurate characterisation of weld microstructures from ultrasonic non-destructive measurements. The subsequent improvement in defect imaging is then demonstrated via use of the DNN predicted crystallographic orientations to correct the delay laws on which the total focusing method imaging algorithm is based. An improvement of up to 5.3 dB in the signal-to-noise ratio is achieved.
Research on Cavitation Characteristics of Two-Throat Nozzle Submerged Jet
Authors: Dong Jingming , Li Shuai , Meng Rongxuan , Zhong Xiao , Pan Xinxiang
Published: January 2022

Ship fouling not only increases ship resistance and fuel consumption but is equally a type of biological invasion, which causes severe ecological damage. Submerged cavitation jet cleaning is an environmentally friendly, high-efficiency, and energy-saving cleaning method. The nozzle structure has an essential influence on the cleaning effect. Thus, a two-throat nozzle was designed for application in submerged cavitation jet cleaning. To investigate the cavitation characteristics of the two-throat nozzle, a high-speed photographic visualization experiment and an erosion experiment concerning the submerged cavitation jet were carried out in this study. The frame-difference method (FDM) was used to analyze the dynamic changes in the cavitation cloud in a single period. The dynamic changes in the cavitation cloud and the characteristics of the submerged cavitation jet were investigated under different inlet pressures. The sample mass loss and the macroscopic and microscopic changes in surface morphology were used to evaluate the cavitation intensity of the two-throat nozzle submerged jet. The experimental results demonstrate that the two-throat nozzle has a good cavitation effect, and the cavitation cloud of the submerged jet has obvious periodicity. With the increase in inlet pressure, the length, width, and area of the cavitation cloud continue to increase, and the shedding frequency of the cavitation cloud continues to decrease. The intensity of cavitation erosion is related to target distance and impact time. There is an appropriate target distance by which to achieve the optimal cavitation effect. The collapse of cavitation bubbles near the sample surface is related to the erosion distribution on the sample surface. Moreover, the magnitude of the absolute values of the root-mean-square surface roughness and surface skewness increase with cavitation intensity. The results in this paper are helpful for a better understanding of the cavitation characteristics of the two-throat nozzle submerged jet.
Mechanical Properties and Leak-Tightness of Polymeric Pipe Adhesive Joints
Authors: Rudawska Anna , Wahab Magd Abdel , Müller Miroslav , Stančeková Dana
Published: January 2022

This paper presents issues related to the determination of the selected mechanical properties of adhesive joints made of polymeric pipes and the evaluation of the leak-tightness of the adhesive joints. The article attempts to demonstrate that the type of adhesive may affect the quality of adhesive joints in terms of both tightness and strength of joints. Five types of the polymer pipes differing in a polypropylene and a polyvinyl chloride, diameter and a wall thickness were used in the experiments. Two types of the adhesives were used to make the adhesive joints: Loctite 3430 A&B Hysol, a two-component epoxy adhesive, and Loctite 406, a one-component cyanoacrylate adhesive. Based on the leak-tightness tests results, it was possible to determine the quality of their adhesive joints without damaging the samples, while their tensile strength was determined through the strength tests. The tests performed allowed for the conclusion that the use of the polyvinyl chloride pipes and Loctite 406 one-component adhesive is recommended for this type of adhesive joints.
Colour and Light in Berlin and Wrocław (Breslau) Department Stores Built between 1927 and 1930
Authors: Kirschke Krystyna , Kirschke Paweł
Published: January 2022

This paper presents the theoretical assumptions and design praxis concerning colour schemes used in the multi-threaded Moderne, Streamline Moderne and Art Deco styles, which were used in Germany during the interwar period to design commercial facilities. We based our analysis on selected cases of department stores built in the years 1927–1930 in Berlin and Wrocław (Breslau at the time). Streamline Moderne and Art Deco, which was present in Germany alongside Expressionism, operated using a simple spatial structure that followed the precepts formulated by the Bauhaus: it featured rhythmically divided, disciplined facades clad in ceramics, sandstone or travertine, as well as large storefront windows with brass frames. These Modernist compositions were enriched with ceramic or brass cornices and friezes, overhangs and full-figure sculptures that were often gilded. The buildings’ interiors, designed following the principles of efficiency and functionality, had spatially accentuated and colour-marked entrance zones and grand, glazed courtyards that were given an expressive décor via ceramics, stone or exotic wood. The expression of these compositions was underscored by linear illumination and cascade-like chandeliers that formed light sculptures. In our paper, we also presented problems associated with the contemporary revitalisation and reconstruction of such buildings. We specifically focused on research findings that identified original ceramics production technologies and methods that allowed the recreation of the texture and colour of the facade of the A. Wertheim department store in Wrocław.
The Last Flemish Primitive: Jan Vercruysse’s Self-Fashioning of Artisthood and National Identity
Authors: Pereira Rodriguez Anton
Published: January 2022

In 1989, the artist Jan Vercruysse (1948–2018) stated that he was “the last Flemish Primitive”. This comment, despite being only a fragment of a lengthy interview with Carolyn Christov-Bakargiev, became a trope in subsequent writings on Vercruysse. I argue that the statement was part of a deliberate strategy by Vercruysse in shaping his identity as a (Belgian) artist. First, I focus on Vercruysse’s Portraits of the Artist (1979–1984), a series of photographic works in which he uses the genre of the self-portrait—thereby implicitly referring to the Flemish Primitives—as a means to express the constructedness of artistic identity. Second, I explore Vercruysse’s construction of his identity and his relationship vis-à-vis the notion of Belgian art. Finally, the statement uttered in 1989 will be contextualized within the changing political and cultural context of Belgium and Flanders in the 1980s. I demonstrate how the statement can be read as invoking a radically different conception of Belgian art during this period.
Long-Range Interactions for Hydrogen Atoms in Excited D States
Authors: Adhikari Chandra M. , Jentschura Ulrich D.
Published: January 2022

Pressure shifts inside an atomic beam are among the more theoretically challenging effects in high-precision measurements of atomic transitions. A crucial element in their theoretical analysis is the understanding of long-range interatomic interactions inside the beam. For excited reference states, the presence of quasi-degenerate states leads to additional challenges, due to the necessity to diagonalize large matrices in the quasi-degenerate hyperfine manifolds. Here, we focus on the interactions of hydrogen atoms in reference states composed of an excited nD state (atom A), and in the metastable 2S state (atom B). We devote special attention to the cases n=3 and n=8. For n=3, the main effect is generated by quasi-degenerate virtual P states from both atoms A and B and leads to experimentally relevant second-order long-range (van-der-Waals) interactions proportional to the sixth inverse power of the interatomic distance. For n=8, in addition to virtual states with two states of P symmetry, one needs to take into account combined virtual P and F states from atoms A and B. The numerical value of the so-called C6 coefficients multiplying the interaction energy was found to grow with the principal quantum number of the reference D state; it was found to be of the order of 1011 in atomic units. The result allows for the calculation of the pressure shift inside atomic beams while driving transitions to nD states.
Characterization of Balance Problems and Rehabilitation Needs of Patients with Ménière’s Disease
Authors: Pyykkö Ilmari , Pyykkö Nora , Zou Jing , Manchaiah Vinaya
Published: January 2022

Background: To explore and characterize balance problems in subjects with Ménière’s disease (MD). Methods: A total of 539 people with MD with a mean age of 61.9 years, mean disease history of 15.6 years, and 79.5% females were recruited. The online questionnaire, consisting of 39 questions, including both structured and open-ended questions, focused on symptoms of MD, balance problems, impacts of the complaints, and quality of life (QoL). Results: After hearing loss (58%) and tinnitus (50%), balance problems (44%) were among the most commonly reported MD complaints, even higher than the impact of vertigo (40%). However, only 22% reported that those balance problems made obvious impacts in their daily lives. The most common balance problem that significantly reduced QoL was tripping (34%). Swaying (25%) had a limited impact on QoL, whereas rocking (10%) was less common but caused a significant impact on QoL. Non-defined balance problems were reported at 18%; these were occasional and correlated with vertigo attacks. Older participants had more frequent tripping problems. Younger participants more frequently reported swaying and rocking. Conclusions: Risk factors predicting poor postural control were mostly related to complaints reflecting otolith pathology. Different types of postural problems require different strategies to manage balance control and cope with the disease.
Development and Performance Measurement of an Affordable Unmanned Surface Vehicle (USV)
Authors: Setiawan Joga Dharma , Septiawan Muhammad Aldi , Ariyanto Mochammad , Caesarendra Wahyu , Munadi M. , Alimi Sabri , Sulowicz Maciej
Published: January 2022

Indonesia is a maritime country that has vast coastal resources and biodiversity. To support the Indonesian maritime program, a topography mapping tool is needed. The ideal topography mapping tool is the Unmanned Surface Vehicle (USV). This paper proposes the design, manufacture, and development of an affordable autonomous USV. The USV which is composed of thruster and rudder is quite complicated to build. This study employs rudderless and double thrusters as the main actuators. PID compensator is utilized as the feedback control for the autonomous USV. Energy consumption is measured when the USV is in autonomous mode. The Dynamics model of USV was implemented to study the roll stability of the proposed USV. Open-source Mission Planner software was selected as the Ground Control Station (GCS) software. Performance tests were carried out by providing the USV with an autonomous mission to follow a specific trajectory. The results showed that the developed USV was able to complete autonomous mission with relatively small errors, making it suitable for underwater topography mapping.
On the Truncated Multidimensional Moment Problems in Cn
Authors: Zagorodnyuk Sergey
Published: January 2022

We consider the problem of finding a (non-negative) measure μ on B(Cn) such that ∫Cnzkdμ(z)=sk, ∀k∈K. Here, K is an arbitrary finite subset of Z+n, which contains (0,…,0), and sk are prescribed complex numbers (we use the usual notations for multi-indices). There are two possible interpretations of this problem. Firstly, one may consider this problem as an extension of the truncated multidimensional moment problem on Rn, where the support of the measure μ is allowed to lie in Cn. Secondly, the moment problem is a particular case of the truncated moment problem in Cn, with special truncations. We give simple conditions for the solvability of the above moment problem. As a corollary, we have an integral representation with a non-negative measure for linear functionals on some linear subspaces of polynomials.
Asymptotic Behavior of Resolvents of a Convergent Sequence of Convex Functions on Complete Geodesic Spaces
Authors: Kimura Yasunori , Shindo Keisuke
Published: January 2022

The asymptotic behavior of resolvents of a proper convex lower semicontinuous function is studied in the various settings of spaces. In this paper, we consider the asymptotic behavior of the resolvents of a sequence of functions defined in a complete geodesic space. To obtain the result, we assume the Mosco convergence of the sets of minimizers of these functions.
Analyzing COVID-19 Medical Papers Using Artificial Intelligence: Insights for Researchers and Medical Professionals
Authors: Soshnikov Dmitry , Petrova Tatiana , Soshnikova Vickie , Grunin Andrey
Published: January 2022

Since the beginning of the COVID-19 pandemic almost two years ago, there have been more than 700,000 scientific papers published on the subject. An individual researcher cannot possibly get acquainted with such a huge text corpus and, therefore, some help from artificial intelligence (AI) is highly needed. We propose the AI-based tool to help researchers navigate the medical papers collections in a meaningful way and extract some knowledge from scientific COVID-19 papers. The main idea of our approach is to get as much semi-structured information from text corpus as possible, using named entity recognition (NER) with a model called PubMedBERT and Text Analytics for Health service, then store the data into NoSQL database for further fast processing and insights generation. Additionally, the contexts in which the entities were used (neutral or negative) are determined. Application of NLP and text-based emotion detection (TBED) methods to COVID-19 text corpus allows us to gain insights on important issues of diagnosis and treatment (such as changes in medical treatment over time, joint treatment strategies using several medications, and the connection between signs and symptoms of coronavirus, etc.).
A Hierarchical Hadoop Framework to Process Geo-Distributed Big Data
Authors: Di Modica Giuseppe , Tomarchio Orazio
Published: January 2022

In the past twenty years, we have witnessed an unprecedented production of data worldwide that has generated a growing demand for computing resources and has stimulated the design of computing paradigms and software tools to efficiently and quickly obtain insights on such a Big Data. State-of-the-art parallel computing techniques such as the MapReduce guarantee high performance in scenarios where involved computing nodes are equally sized and clustered via broadband network links, and the data are co-located with the cluster of nodes. Unfortunately, the mentioned techniques have proven ineffective in geographically distributed scenarios, i.e., computing contexts where nodes and data are geographically distributed across multiple distant data centers. In the literature, researchers have proposed variants of the MapReduce paradigm that obtain awareness of the constraints imposed in those scenarios (such as the imbalance of nodes computing power and of interconnecting links) to enforce smart task scheduling strategies. We have designed a hierarchical computing framework in which a context-aware scheduler orchestrates computing tasks that leverage the potential of the vanilla Hadoop framework within each data center taking part in the computation. In this work, after presenting the features of the developed framework, we advocate the opportunity of fragmenting the data in a smart way so that the scheduler produces a fairer distribution of the workload among the computing tasks. To prove the concept, we implemented a software prototype of the framework and ran several experiments on a small-scale testbed. Test results are discussed in the last part of the paper.
The Neonicotinoid Thiacloprid Interferes with the Development, Brain Antioxidants, and Neurochemistry of Chicken Embryos and Alters the Hatchling Behavior: Modulatory Potential of Phytochemicals
Authors: Farag Mayada R. , Alagawany Mahmoud , Moselhy Attia A. A. , Said Enas N. , Ismail Tamer A. , Di Cerbo Alessandro , Pugliese Nicola , Ahmed Mona M.
Published: January 2022

The present experiment was performed to investigate the toxic impact of thiacloprid (TH) on the brain of developing chicken embryos and also to measure its influence on the behavioral responses of hatchlings. The role of chicoric acid (CA) and rosmarinic acid (RA) in modulating the resulted effects was also investigated. The chicken eggs were in ovo inoculated with TH at different doses (0.1, 1, 10, and 100 ug/egg). TH increased the mortality and abnormality rates and altered the neurochemical parameters of exposed embryos dose-dependently. TH also decreased the brain level of monoamines and amino acid neurotransmitters and decreased the activities of acetylcholine esterase (AchE) and Na+/K+-ATPase. The brain activity of catalase (CAT) and superoxide dismutase (SOD) was diminished with downregulation of their mRNA expressions in the brain tissue. When TH was co-administered with CA and RA, the toxic impacts of the insecticide were markedly attenuated, and they showed a complementary effect when used in combination. Taken together, these findings suggested that TH is neurotoxic to chicken embryos and is possibly neurotoxic to embryos of other vertebrates. The findings also demonstrated the antioxidant and neuroprotective effects of CA and RA. Based on the present findings, the CA and RA can be used as invaluable ameliorative of TH-induced toxicity.
Effectiveness of Olympic Combat Sports on Balance, Fall Risk or Falls in Older Adults: A Systematic Review
Authors: Valdés-Badilla Pablo , Ramirez-Campillo Rodrigo , Herrera-Valenzuela Tomás , Branco Braulio Henrique Magnani , Guzmán-Muñoz Eduardo , Mendez-Rebolledo Guillermo , Concha-Cisternas Yeny , Hernandez-Martínez Jordan
Published: January 2022

This systematic review and meta-analysis aimed to assess the available body of published peer-reviewed articles related to the effects of Olympic combat sports (OCS), compared with active/passive controls, on balance, fall risk, or falls in older adults. The TESTEX and GRADE scales assessed the methodological quality and certainty of the evidence. The protocol was registered in PROSPERO (code: CRD42020204034). From 1496 records, eight studies were included, involving 322 older adults (64% female; mean age = 71.1 years). The TESTEX scale revealed all studies with a score ≥ 60% (moderate-high quality). The GRADE scale indicated all studies with at least some concerns, up to a high risk of bias (i.e., was rated very low). Meta-analyses were planned, although the reduced number of studies precluded its incorporation in the final manuscript. Only two from six studies that assessed balance found improvements after OCS compared to controls. No differences were found between OCS vs. control groups for fall risk or falls. The available evidence does not allow a definitive recommendation for or against OCS interventions as an effective strategy to improve balance and reduce the fall risk or falls in older adults. Therefore, more high-quality studies are required to draw definitive conclusions.
Long-Term Combined Effects of Citrulline and Nitrate-Rich Beetroot Extract Supplementation on Recovery Status in Trained Male Triathletes: A Randomized, Double-Blind, Placebo-Controlled Trial
Authors: Burgos José , Viribay Aitor , Calleja-González Julio , Fernández-Lázaro Diego , Olasagasti-Ibargoien Jurgi , Seco-Calvo Jesús , Mielgo-Ayuso Juan
Published: January 2022

Citrulline (CIT) and nitrate-rich beetroot extract (BR) are widely studied ergogenic aids. Nevertheless, both supplements have been studied in short-term trials and separately. To the best of the authors’ knowledge, the effects of combining CIT and BR supplementation on recovery status observed by distance covered in the Cooper test, exercise-induced muscle damage (EIMD) and anabolic/catabolic hormone status have not been investigated to date. Therefore, the main purpose of this research was to assess the effect of the long-term (9 weeks) mixture of 3 g/day of CIT plus 2.1 g/day of BR (300 mg/day of nitrates (NO3−)) supplementation on recovery by distance covered in the Cooper test, EIMD markers (urea, creatinine, AST, ALT, GGT, LDH and CK) and anabolic/catabolic hormones (testosterone, cortisol and testosterone/cortisol ratio (T/C)) in male trained triathletes. Thirty-two triathletes were randomized into four different groups of eight triathletes in this double-blind, placebo-controlled trial: placebo group (PLG), CIT group (CITG; 3 g/day of CIT), BR group (BRG; 2.1 g/day of BR (300 mg/day of NO3−)) and CIT-BR group (CIT-BRG; 3 g/day of CIT plus 2.1 g/day of BR (300 mg/day of NO3−)). Distance covered in the Cooper test and blood samples were collected from all participants at baseline (T1) and after 9 weeks of supplementation (T2). There were no significant differences in the interaction between group and time in EIMD markers (urea, creatinine, AST, ALT, GGT, LDH and CK) (p > 0.05). However, significant differences were observed in the group-by-time interaction in distance covered in the Cooper test (p = 0.002; η2p = 0.418), cortisol (p = 0.044; η2p = 0.247) and T/C (p = 0.005; η2p = 0.359). Concretely, significant differences were observed in distance covered in the Cooper test percentage of change (p = 0.002; η2p = 0.418) between CIT-BRG and PLG and CITG, in cortisol percentage change (p = 0.049; η2p = 0.257) and in T/C percentage change (p = 0.018; η2p = 0.297) between CIT-BRG and PLG. In conclusion, the combination of 3 g/day of CIT plus 2.1 g/day of BR (300 mg/day of NO3−) supplementation for 9 weeks did not present any benefit for EIMD. However, CIT + BR improved recovery status by preventing an increase in cortisol and showing an increase in distance covered in the Cooper test and T/C.
ALS2-Related Motor Neuron Diseases: From Symptoms to Molecules
Authors: Miceli Marcello , Exertier Cécile , Cavaglià Marco , Gugole Elena , Boccardo Marta , Casaluci Rossana Rita , Ceccarelli Noemi , De Maio Alessandra , Vallone Beatrice , Deriu Marco A.
Published: January 2022

Infantile-onset Ascending Hereditary Spastic Paralysis, Juvenile Primary Lateral Sclerosis and Juvenile Amyotrophic Lateral Sclerosis are all motor neuron diseases related to mutations on the ALS2 gene, encoding for a 1657 amino acids protein named Alsin. This ~185 kDa multi-domain protein is ubiquitously expressed in various human tissues, mostly in the brain and the spinal cord. Several investigations have indicated how mutations within Alsin’s structured domains may be responsible for the alteration of Alsin’s native oligomerization state or Alsin’s propensity to interact with protein partners. In this review paper, we propose a description of differences and similarities characterizing the above-mentioned ALS2-related rare neurodegenerative disorders, pointing attention to the effects of ALS2 mutation from molecule to organ and at the system level. Known cases were collected through a literature review and rationalized to deeply elucidate the neurodegenerative clinical outcomes as consequences of ALS2 mutations.
Role of Ajwa Date Fruit Pulp and Seed in the Management of Diseases through In Vitro and In Silico Analysis
Authors: Anwar Shehwaz , Raut Ravindra , Alsahli Mohammed A. , Almatroudi Ahmad , Alfheeaid Hani , Alzahrani Faisal M. , Khan Amjad Ali , Allemailem Khaled S. , Almatroodi Saleh A. , Rahmani Arshad Husain
Published: January 2022

This study investigated the health-promoting activities of methanolic extracts of Ajwa date seed and fruit pulp extracts through in vitro studies. These studies confirmed potential antioxidant, anti-hemolytic, anti-proteolytic, and anti-bacterial activities associated with Ajwa dates. The EC50 values of fruit pulp and seed extracts in methanol were reported to be 1580.35 ± 0.37 and 1272.68 ± 0.27 µg/mL, respectively, in the DPPH test. The maximum percentage of hydrogen peroxide-reducing activity was 71.3 and 65.38% for both extracts at 600 µg/mL. Fruit pulp and seed extracts inhibited heat-induced BSA denaturation by 68.11 and 60.308%, heat-induced hemolysis by 63.84% and 58.10%, and hypersalinity-induced hemolysis by 61.71% and 57.27%, and showed the maximum anti-proteinase potential of 56.8 and 51.31% at 600 μg/mL, respectively. Seed and fruit pulp inhibited heat-induced egg albumin denaturation at the same concentration by 44.31 and 50.84%, respectively. Ajwa seed showed minimum browning intensity by 63.2%, percent aggregation index by 64.2%, and amyloid structure by 63.8% at 600 μg/mL. At 100 mg/mL, Ajwa seed extract exhibited good antibacterial activity. Molecular docking analysis showed that ten active constituents of Ajwa seeds bind with the critical antioxidant enzymes, catalase (1DGH) and superoxide dismutase (5YTU). The functional residues involved in such interactions include Arg72, Ala357, and Leu144 in 1DGH, and Gly37, Pro13, and Asp11 in 5YTU. Hence, Ajwa dates can be used to develop a suitable alternative therapy in various diseases, including diabetes and possibly COVID-19-associated complications.
Sex Differences in Animal Models of Sodium-Valproate-Induced Autism in Postnatal BALB/c Mice: Whole-Brain Histoarchitecture and 5-HT2A Receptor Biomarker Evidence
Authors: Gouda Balaji , Sinha Sukesh Narayan , Chalamaiah Meram , Vakdevi Validandi , Shashikala Patangay , Veeresh Bantal , Surekha Venkata Mullapudi , Kasturi Vasudev , Boiroju Naveen Kumar
Published: January 2022

Autism spectrum disorder (ASD) is characterised by problems with social interaction, verbal and nonverbal communication and repetitive behaviour. In mice, the 14th postnatal day is believed to correspond to the third trimester of human embryonic development and is considered a vital period for central nervous system development. It has been shown that ASD affects 2 to 3 times more male than female individuals. In the present study, ASD was induced in 14 postnatal day (PND) BALB/c mice using valproic acid (VPA). VPA administration brought about substantial differences in the histoarchitecture of the brain in both male and female mice, linked to behavioural deficits. We observed that both male and female mice showed similar morphological changes in the prefrontal cortex, hippocampus and Purkinje cells. We also observed hair loss from PND 17 to 25, which was again similar between male and female mice. However, there were higher rates of change in the cerebral cortex, frontal cortex and temporal lobe and hippocampus in VPA-treated male animals. With respect to the cerebellum, we did not observe any alterations by haematoxylin and eosin (H&E) staining, but detailed morphological observation using scanning electron microscopy (SEM) showed a higher rate of phenotype changes in VPA-treated male animals. Moreover, 5-HT2A receptor protein levels were upregulated in the cerebral cortex, hippocampus and Purkinje cells in VPA-treated male mice compared with control animals and VPA-treated female mice, as shown by immunohistochemical analysis. Based on all these findings, we conclude that male animals are more susceptible to VPA-induced ASD than females.
Association between Soluble α-Klotho Protein and Metabolic Syndrome in the Adult Population
Authors: Cheng Yung-Wen , Hung Chun-Chi , Fang Wen-Hui , Chen Wei-Liang
Published: January 2022

Klotho protein is an anti-aging protein and plays multiple roles in ion-regulation, anti-oxidative stress, and energy metabolism through various pathways. Metabolic syndrome is a combination of multiple conditions that compose of multiple risk factors of cardiovascular disease and type 2 diabetes. Gene regulation and protein expression are discovered associated with metabolic syndrome. We aimed to figure out the correlation between Klotho protein and metabolic syndrome in generally healthy adults. A cross-sectional study of 9976 respondents ≥ 18 years old from the US National Health and Nutrition Examination Survey (2007–2012) by utilizing their soluble Klotho protein concentrations. Multivariate linear regression models were used to analyze the effect of soluble Klotho protein on the prevalence of metabolic syndrome. Soluble Klotho protein concentration was inversely correlated with the presence of metabolic syndromes (p = 0.013) and numbers of components that met the definition of metabolic syndrome (p < 0.05). The concentration of Soluble Klotho protein was negatively associated with abdominal obesity and high triglyceride (TG) in the adjusted model (p < 0.05). Soluble Klotho protein is correlated with changing metabolic syndrome components in adults, especially central obesity and high TG levels. Despite conventional function as co-factor with fibroblast growth factor-23 (FGF23) that regulates phosphate and vitamin D homeostasis, FGF23-independent soluble Klotho protein may act on multiple signal pathways in different organs and tissue in roles of anti-aging and protection from metabolic syndrome.
Calcium Signaling Pathway Is Involved in the Shedding of ACE2 Catalytic Ectodomain: New Insights for Clinical and Therapeutic Applications of ACE2 for COVID-19
Authors: García-Escobar Artemio , Vera-Vera Silvio , Jurado-Román Alfonso , Jiménez-Valero Santiago , Galeote Guillermo , Moreno Raúl
Published: January 2022

The angiotensin-converting enzyme 2 (ACE2) is a type I integral membrane that exists in two forms: the first is a transmembrane protein; the second is a soluble catalytic ectodomain of ACE2. The catalytic ectodomain of ACE2 undergoes shedding by a disintegrin and metalloproteinase domain-containing protein 17 (ADAM17), in which calmodulin mediates the calcium signaling pathway that is involved in ACE2 release, resulting in a soluble catalytic ectodomain of ACE2 that can be measured as soluble ACE2 plasma activity. The shedding of the ACE2 catalytic ectodomain plays a role in cardiac remodeling and endothelial dysfunction and is a predictor of all-cause mortality, including cardiovascular mortality. Moreover, considerable evidence supports that the ACE2 catalytic ectodomain is an essential entry receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Additionally, endotoxins and the pro-inflammatory cytokines interleukin (IL)-1β and tumor necrosis factor-alpha (TNFα) all enhanced soluble catalytic ectodomain ACE2 shedding from the airway epithelia, suggesting that the shedding of ACE2 may represent a mechanism by which viral entry and infection may be controlled such as some types of betacoronavirus. In this regard, ACE2 plays an important role in inflammation and thrombotic response, and its down-regulation may aggravate COVID-19 via the renin-angiotensin system, including by promoting pathological changes in lung injury. Soluble forms of ACE2 have recently been shown to inhibit SARS-CoV-2 infection. Furthermore, given that vitamin D enhanced the shedding of ACE2, some studies reported that vitamin D treatment is associated with prognosis improvement in COVID-19. This is an updated review on the evidence, clinical, and therapeutic applications of ACE2 for COVID-19.
In-Depth Molecular Dynamics Study of All Possible Chondroitin Sulfate Disaccharides Reveals Key Insight into Structural Heterogeneity and Dynamism
Authors: Nagarajan Balaji , Sankaranarayanan Nehru Viji , Desai Umesh R.
Published: January 2022

GAGs exhibit a high level of conformational and configurational diversity, which remains untapped in terms of the recognition and modulation of proteins. Although GAGs are suggested to bind to more than 800 biologically important proteins, very few therapeutics have been designed or discovered so far. A key challenge is the inability to identify, understand and predict distinct topologies accessed by GAGs, which may help design novel protein-binding GAG sequences. Recent studies on chondroitin sulfate (CS), a key member of the GAG family, pinpointing its role in multiple biological functions led us to study the conformational dynamism of CS building blocks using molecular dynamics (MD). In the present study, we used the all-atom GLYCAM06 force field for the first time to explore the conformational space of all possible CS building blocks. Each of the 16 disaccharides was solvated in a TIP3P water box with an appropriate number of counter ions followed by equilibration and a production run. We analyzed the MD trajectories for torsional space, inter- and intra-molecular H-bonding, bridging water, conformational spread and energy landscapes. An in-house phi and psi probability density analysis showed that 1→3-linked sequences were more flexible than 1→4-linked sequences. More specifically, phi and psi regions for 1→4-linked sequences were held within a narrower range because of intra-molecular H-bonding between the GalNAc O5 atom and GlcA O3 atom, irrespective of sulfation pattern. In contrast, no such intra-molecular interaction arose for 1→3-linked sequences. Further, the stability of 1→4-linked sequences also arose from inter-molecular interactions involving bridged water molecules. The energy landscape for both classes of CS disaccharides demonstrated increased ruggedness as the level of sulfation increased. The results show that CS building blocks present distinct conformational dynamism that offers the high possibility of unique electrostatic surfaces for protein recognition. The fundamental results presented here will support the development of algorithms that help to design longer CS chains for protein recognition.
Genetic and Histopathological Heterogeneity of Neuroblastoma and Precision Therapeutic Approaches for Extremely Unfavorable Histology Subgroups
Authors: Shimada Hiroyuki , Ikegaki Naohiko
Published: January 2022

Peripheral neuroblastic tumors (neuroblastoma, ganglioneuroblastoma and ganglioneuroma) are heterogeneous and their diverse and wide range of clinical behaviors (spontaneous regression, tumor maturation and aggressive progression) are closely associated with genetic/molecular properties of the individual tumors. The International Neuroblastoma Pathology Classification, a biologically relevant and prognostically significant morphology classification distinguishing the favorable histology (FH) and unfavorable histology (UH) groups in this disease, predicts survival probabilities of the patients with the highest hazard ratio. The recent advance of neuroblastoma research with precision medicine approaches demonstrates that tumors in the UH group are also heterogeneous and four distinct subgroups—MYC, TERT, ALT and null—are identified. Among them, the first three subgroups are collectively named extremely unfavorable histology (EUH) tumors because of their highly aggressive clinical behavior. As indicated by their names, these EUH tumors are individually defined by their potential targets detected molecularly and immunohistochemically, such as MYC-family protein overexpression, TERT overexpression and ATRX (or DAXX) loss. In the latter half on this paper, the current status of therapeutic targeting of these EUH tumors is discussed for the future development of effective treatments of the patients.
Phytochemistry and Diverse Pharmacology of Genus Mimosa: A Review
Authors: Rizwan Komal , Majeed Ismat , Bilal Muhammad , Rasheed Tahir , Shakeel Ahmad , Iqbal Shahid
Published: January 2022

The genus Mimosa belongs to the Fabaceae family and comprises almost 400 species of herbs, shrubs and ornamental trees. The genus Mimosa is found all over the tropics and subtropics of Asia, Africa, South America, North America and Australia. Traditionally, this genus has been popular for the treatment of jaundice, diarrhea, fever, toothache, wound healing, asthma, leprosy, vaginal and urinary complaints, skin diseases, piles, gastrointestinal disorders, small pox, hepatitis, tumor, HIV, ulcers and ringworm. The review covered literature available from 1959 to 2020 collected from books, scientific journals and electronic searches, such as Science Direct, Web of Science and Google scholar. Various keywords, such as Mimosa, secondary metabolites, medicines, phytochemicals and pharmacological values, were used for the data search. The Mimosa species are acknowledged to be an essential source of secondary metabolites with a wide-ranging biological functions, and up until now, 145 compounds have been isolated from this genus. Pharmacological studies showed that isolated compounds possess significant potential, such as antiprotozoal, antimicrobial, antiviral, antioxidant, and antiproliferative as well as cytotoxic activities. Alkaloids, chalcones, flavonoids, indoles, terpenes, terpenoids, saponins, steroids, amino acids, glycosides, flavanols, phenols, lignoids, polysaccharides, lignins, salts and fatty esters have been isolated from this genus. This review focused on the medicinal aspects of the Mimosa species and may provide a comprehensive understanding of the prospective of this genus as a foundation of medicine, supplement and nourishment. The plants of this genus could be a potential source of medicines in the near future.
Towards Mapping of the Human Brain N-Glycome with Standardized Graphitic Carbon Chromatography
Authors: Helm Johannes , Hirtler Lena , Altmann Friedrich
Published: January 2022

The brain N-glycome is known to be crucial for many biological functions, including its involvement in neuronal diseases. Although large structural studies of brain N-glycans were recently carried out, a comprehensive isomer-specific structural analysis has still not been achieved, as indicated by the recent discovery of novel structures with galactosylated bisecting GlcNAc. Here, we present a detailed, isomer-specific analysis of the human brain N-glycome based on standardized porous graphitic carbon (PGC)-LC-MS/MS. To achieve this goal, we biosynthesized glycans with substitutions typically occurring in the brain N-glycome and acquired their normalized retention times. Comparison of these values with the standardized retention times of neutral and desialylated N-glycan fractions of the human brain led to unambiguous isomer specific assignment of most major peaks. Profound differences in the glycan structures between naturally neutral and desialylated glycans were found. The neutral and sialylated N-glycans derive from diverging biosynthetic pathways and are biosynthetically finished end products, rather than just partially processed intermediates. The focus on structural glycomics defined the structure of human brain N-glycans, amongst these are HNK-1 containing glycans, a bisecting sialyl-lactose and structures with fucose and N-acetylgalactosamine on the same arm, the so-called LDNF epitope often associated with parasitic worms.
Morphological, Gene, and Hormonal Changes in Gonads and In-Creased Micrococcal Nuclease Accessibility of Sperm Chromatin Induced by Mercury
Authors: Lettieri Gennaro , Carusone Nadia , Notariale Rosaria , Prisco Marina , Ambrosino Alessia , Perrella Shana , Manna Caterina , Piscopo Marina
Published: January 2022

Mercury is one of the most dangerous environmental pollutants. In this work, we analysed the effects of exposure of Mytilus galloprovincialis to 1, 10 and 100 pM HgCl2 for 24 h on the gonadal morphology and on the expression level of three stress genes: mt10, hsp70 and πgst. In this tissue we also evaluated the level of steroidogenic enzymes 3β-HSD and 17β-HSD and the expression of PL protein genes. Finally, we determined difference in sperm chromatin accessibility to micrococcal nuclease. We found alterations in gonadal morphology especially after exposure to 10 and 100 pM HgCl2 and hypo-expression of the three stress genes, particularly for hsp70. Furthermore, decreased labelling with both 3β-HSD and 17β-HSD antibodies was observed following exposure to 1 and 10 pM HgCl2 and complete absence at 100 pM HgCl2 exposure. Gonads of mussels exposed to all HgCl2 doses showed decreased expression of PL protein genes especially for PLIII. Finally, micrococcal nuclease digestions showed that all doses of HgCl2 exposure resulted in increased sperm chromatin accessibility to this enzyme, indicative of improper sperm chromatin structure. All of these changes provide preliminary data of the potential toxicity of mercury on the reproductive health of this mussel.