bluuebunny/crossref_metadata_2025 huggingface dataset:
Masculinity and Leadership Effectiveness (Self-)Perceptions: The Case of Lesbian Leaders
Authors: Shamloo Soraya Elizabeth , De Cristofaro Valeria , Pellegrini Valerio , Salvati Marco
Published: December 2022

In line with the gay glass ceiling effect, sexual minorities are often target of discrimination within work-related contexts, thus potentially undermining their wellbeing at work. For gay men, discrimination may partially be attributed to gay men’s stereotypical feminine perception, which does not fit with the stereotypically masculine traits required for leadership positions. Yet, when considering lesbian women, the masculine stereotypical view associated with them may come to represent an advantage in work-related contexts, especially when compared to heterosexual women. In Study 1, N = 303 heterosexual participants rated a lesbian vs. a heterosexual woman as a job candidate on stereotypical gender (masculine vs. feminine) traits as well as leadership effectiveness. Results showed that being lesbian was associated with higher levels of masculinity (but not femininity), which in turn was related to high leadership effectiveness. In Study 2, N = 268 lesbian and heterosexual women rated themselves on the same measures. Results showed that both groups associated masculine traits with enhanced leadership effectiveness. These studies provide a better comprehension regarding how lesbian women may be perceived in work-related contexts and shed light on the role played by gender stereotypical perceptions in shaping both heterosexual and lesbian perceptions of leadership effectiveness.
Reasons for Altering Bladder Management and Satisfaction with Current Bladder Management in Chronic Spinal Cord Injury Patients
Authors: Yeh Hui-Ling , Kuo Hann-Chorng , Tsai Chuan-Hsiu , Lee Ru-Ping
Published: December 2022

Patients with spinal cord injury (SCI) often require bladder management. However, patients routinely change their bladder management for better satisfaction. The reasons for altering a bladder management method in SCI patients remain insufficiently understood. The purposes of this study are to assess current satisfaction with bladder management and the reasons for changing bladder management in SCI patients. A prospective cross-sectional survey with a convenience sampling method was used. The study was conducted from January 2018 to December 2019. The inclusion criteria included an age ≥18 years and a diagnosis of SCI more than one year previously. The questionnaires were self-administered and collected from eligible patients during a free clinic service. A total of 515 SCI participants were enrolled. Two hundred and eighty-three (55.0%) participants had experienced changing their bladder management. The most used method of current bladder management was self-voiding. About 84.7% of participants reported being satisfied with their current bladder management. Bladder management changes were most often made due to frequent urinary tract infections. Furthermore, the participants dissatisfied with their management had more urological complications. This study indicates that appropriate bladder management can improve the subjective satisfaction of patients. For long-term care, preventing urinary tract infections is a helpful strategy for patients’ satisfaction with bladder management.
Recommendations for Writing Clinical Research Manuscripts: From Monofocal to Multifocal Intraocular Lenses
Authors: Fernández Joaquín , Rocha-De-Lossada Carlos , Rodríguez-Vallejo Manuel
Published: December 2022

Writing a manuscript is not an easy task, and publishing in peer-reviewed journals might prove difficult if the methodology is not appropriately described and results are not clearly presented [...]
Implicit Negativity Bias Leads to Greater Loss Aversion and Learning during Decision-Making
Authors: Molins Francisco , Martínez-Tomás Celia , Serrano Miguel Ángel
Published: December 2022

It is widely accepted there is the existence of negativity bias, a greater sensitivity to negative emotional stimuli compared with positive ones, but its effect on decision-making would depend on the context. In risky decisions, negativity bias could lead to non-rational choices by increasing loss aversion; yet in ambiguous decisions, it could favor reinforcement-learning and better decisions by increasing sensitivity to punishments. Nevertheless, these hypotheses have not been tested to date. Our aim was to fill this gap. Sixty-nine participants rated ambiguous emotional faces (from the NimStim set) as positive or negative to assess negativity bias. The implicit level of the bias was also obtained by tracking the mouse’s trajectories when rating faces. Then, they performed both a risky and an ambiguous decision-making task. Participants displayed negativity bias, but only at the implicit level. In addition, this bias was associated with loss aversion in risky decisions, and with greater performance through the ambiguous decisional task. These results highlight the need to contextualize biases, rather than draw general conclusions about whether they are inherently good or bad.
A Forehead Wearable Sensor for the Objective Measurement of Chronic Pain
Authors: Orzabal Marcus , Naidu Ramo , Amirdelfan Kasra , Akhbardeh Alireza
Published: December 2022

Chronic pain impacts one in five Americans and is difficult to manage, costing ~USD 600 billion annually. The subjective experience of pain is a complex processing of central nervous system input. Recent advances in magnetic resonance imaging revealed the prefrontal cortex as vital to the perception of pain and that changes in the cerebral hemodynamics can be used to detect painful sensations. Current pain monitoring is dependent on the subjective rating provided by patients and is limited to a single time point. We have developed a biomarker for the objective, real-time and continuous chronic pain assessment using proprietary algorithms termed ROPA and cerebral optical spectrometry. Using a forehead sensor, the cerebral optical spectrometry data were collected in two clinical sites from 41 patients (19 and 22, respectively, from sites 1 and 2), who elected to receive an epidural steroid injection for the treatment of chronic pain. Patients rated their pain on a numeric rating scale, ranging from 0–10, which were used to validate the ROPA objective pain scoring. Multiple time points, including pre- and post-procedure were recorded. The steroid injection was performed per standard medical practice. There was a significant correlation between the patient’s reported numeric rating scale and ROPA, for both clinical sites (Overall ~0.81). Holding the subjective pain ratings on a numeric rating scale as ground truth, we determined that the area under the receiver operator curves for both sites revealed at least good (AUC: 64%) to excellent (AUC > 98%) distinctions between clinically meaningful pain severity differentiations (no/mild/moderate/severe). The objective measure of chronic pain (ROPA) determined using cerebral optical spectrometry significantly correlated with the subjective pain scores reported by the subjects. This technology may provide a useful method of detection for the objective and continuous monitoring and treatment of patients with chronic pain, particularly in clinical circumstances where direct assessment is not available, or to complement the patient-reported pain scores.
Study on the Influence of Mining Activities on the Quality of Deep Karst Groundwater Based on Multivariate Statistical Analysis and Hydrochemical Analysis
Authors: Li Chen , Gui Herong , Guo Yan , Chen Jiayu , Li Jun , Xu Jiying , Yu Hao
Published: December 2022

Long-term mining activities have changed the hydrogeochemical evolution process of groundwater and threatened the safe use of groundwater. By using the methods of hydrochemistry and multivariate statistical analysis, this study determined the hydrogeochemical evolution mechanism affecting the quality of karst groundwater by analyzing the conventional hydrochemistry data of the karst groundwater of the Carboniferous Taiyuan Formation in Hengyuan Coal Mine in the recent 12 years. The results show that, under the disturbance of mining, the quality of karst groundwater in Taiyuan Formation is poor, mainly because the contents of Na++K+ and SO42− are too high to allow usage as drinking water. The reason for the high content of SO42− in karst groundwater lies in the dissolution of gypsum and the oxidation of pyrite, and the high content of Na++K+ lies in the cation exchange. Influenced by the stratum grouting, the circulation of karst groundwater is improved, the cation exchange is weakened, and the desulfurization is enhanced. In the future, it is predicted that the hydrochemical type of karst groundwater in Taiyuan Formation in the study area will evolve from SO4-Ca·Mg type to HCO3-Ca Mg type.
Similarity Analysis in Understanding Online News in Response to Public Health Crisis
Authors: Cezario Sidemar , Marques Thiago , Pinto Rafael , Lacerda Juciano , Silva Lyrene , Santos Lima Thaisa , Santana Orivaldo , Ribeiro Anna Giselle , Cruz Agnaldo , Araújo Ana Claudia , Miranda Angélica Espinosa , Cadaxa Aedê , Teixeira César , Muñoz Almudena , Valentim Ricardo
Published: December 2022

Background: The “Syphilis No!” campaign the Brazilian Ministry of Health (MoH) launched between November 2018 and March 2019, brought forward the concept "Test, Treat and Cure" to remind the population of the importance of syphilis prevention. In this context, this study aims to analyze the similarity of syphilis online news to comprehend how public health communication interventions influence media coverage of the syphilis issue. Methods: This paper presented a computational approach to assess the effectiveness of communication actions on a public health problem. Data were collected between January 2015 and December 2019 and processed using the Hermes ecosystem, which utilizes text mining and machine learning algorithms to cluster similar content. Results: Hermes identified 1049 google-indexed web pages containing the term ’syphilis’ in Brazil. Of these, 619 were categorized as news stories. In total, 157 were grouped into clusters of at least two similar news items and a single cluster with 462 news classified as “single” for not featuring similar news items. From these, 19 clusters were identified in the pre-campaign period, 23 during the campaign, and 115 in the post-campaign. Conclusions: The findings presented in this study show that the volume of syphilis-related news reports has increased in recent years and gained popularity after the SNP started, having been boosted during the campaign and escalating even after its completion.
Integrating Spatial Heterogeneity into an Analysis between Ecosystem Service Value and Its Driving Factors: A Case Study of Dalian, China
Authors: Zhao Yuqing , Han Zenglin , Yan Xiaolu , Wang Xuezhe
Published: December 2022

Human demand for natural resources has grown, leading to ecological debasement and related ecological system administration. Using Dalian as an example, we estimated the changes in the ecosystem service value (ESV) in 2005 and 2020. We used ArcGIS and spatial statistics to conduct estimations and change analyses of the ESV. Based on the results of the ESV, the geographical detector and geographically weighted regression (GWR) elucidated the contributions of different driving factors of the ESV in a 2 km grid. In summary, these results indicated that: (1) from a holistic perspective, the ESV of Dalian fell by 206.8009 billion CNY over 15 years, and the hot spots were concentrated in both the northern and the western parts, whereas the cold spots were distributed in the central part; (2) according to the results from the geographical detector, land use structure factors influenced the ESV most significantly, followed by socio-economic factors, and the impact of natural factors was relatively small; and (3) according to the results of the GWR, land use structure factors negatively affected the ESV, and the positive impact of the proportion of the natural land area was the most obvious. We conclude that the decline in the ESV reflects the impact of human activities on the ecosystem in the studied landscape. Understanding ESV changes should be made a priority in ecosystem management, and evaluating ESV drivers can contribute to developing land use strategies for policy-making.
Leaching Characteristics of Potentially Toxic Metals from Tailings at Lujiang Alum Mine, China
Authors: Zhu Hongyan , Xu Jinbo , Zhou Beibei , Ren Jia , Yang Qiang , Wang Zhe , Nie Weibo
Published: December 2022

To investigate the leaching characteristics and potential environmental effects of potentially toxic metals (PTMs) from alum mine tailings in Lujiang, Anhui Province, soaking tests and simulated rainfall leaching experiments were conducted for two types of slag. PTMs comprising Cd, Cr, Cu, Mn, and Ni were detected in the slag. Cu and Cd contents exceeded the national soil risk screening values (GB 15618-2018). pH values of the two slag soaking solutions were negatively correlated with the solid:liquid ratio. pH values of the sintered slag soaking solutions with different solid:liquid ratios finally stabilized between 4.4 and 4.59, and those of the waste slag soaking solutions finally stabilized between 2.7 and 3.4. The concentrations of Cd, Cr, Cu, Mn, and Ni leached from waste slag were higher than those from sintered slag, and the dissolved concentrations of these PTMs in sintered slag were higher under rainfall leaching conditions than soaking conditions (the difference in Cr concentration was the smallest, 5.6%). The cumulative release of Cd, Cr, Cu, Mn, and Ni increased as the leaching liquid volume increased. The kinetic characteristics of the cumulative release of the five PTMs were best fitted by a double constant equation (R2 > 0.98 for all fits). Single factor index evaluations showed that Mn and Ni were the PTMs with high pollution degrees (Pi for Mn and Ni exceed 1) in the leaching solutions. However, considering the biotoxicity of PTMs, the water quality index evaluations showed that the water quality of the sintered slag soaking solution, the waste slag soaking solution, and the sintered slag leachate was good, poor, and undrinkable, respectively. The health risk assessment showed that the total non-carcinogenic risk (HI) values in adults for both the sintered slag leachate and waste slag soaking solution exceeded the safe level of 1, with HI values of 3.965 and 2.342, respectively. The hazard quotient (HQ) for Cd was 1.994 for the sintered slag leachate, and Cd and Cr make up 50.29% and 15.93% of the total risk, respectively. Cr makes up 28.38% of the total risk for the waste slag soaking solution. These results indicate a high non-carcinogenic risk of exposure to Cd and Cr in the leaching solution used for drinking purposes. These findings may provide a reference for the evaluation and ecological control of PTM pollution in alum mining areas.
Differences in Adolescent’s Cardiometabolic Health: A Comparison Regarding Guided Team and Endurance Sports
Authors: Martin Ștefan Adrian , Martin-Hadmaș Roxana Maria
Published: December 2022

Physical exercise can induce changes in gas exchange and ventilation kinetics. Of these, some will lead to various adaptations which can influence performance and health. We conducted a single-center observational study on 40 participants. Of them, 20 participants came from volleyball (Sample 1) and the other 20 participants came from triathlon (Sample 2). All participants underwent anthropometric and basal metabolic rate measurements, along with a laboratory aerobic capacity test (VO2peak). In Sample 1, the VO2peak was 2.38 L/min and 37.5 mL/min/kg, unlike in Sample 2, which had 2.31 L/min (p = 0.402, Mann–Whitney U = 204) and 43 mL/min/kg oxygen uptake (p = 0.0001). VE/VO2 was significantly different (p = 0.0002, Mann–Whitney U = 80), reaching 31.3 and 36.1 L/min in Sample 1 and Sample 2. Similar results were obtained regarding VE/VCO2 (p = 0.0074, Mann–Whitney U = 126), i.e., 31.3 and 33.9 L/min in Sample 1 and Sample 2. The contribution of physical activity was observed in both groups by taking into account the peak oxygen uptake. In comparison, the team sports sample showed an increased metabolic cost at the VO2peak.
Lung Support Service: Implementation of a Nationwide Text Message Support Program for People with Chronic Respiratory Disease during the COVID-19 Pandemic
Authors: Raeside Rebecca , Singleton Anna C. , Todd Allyson , Partridge Stephanie R. , Hyun Karice K. , Kulas Helen , Wootton Sally L. , Dale Marita T. , Alison Jennifer A. , McKeough Zoe , McNamara Renae J. , Spencer Lissa , Jenkins Christine , Redfern Julie
Published: December 2022

Background: COVID-19 pandemic lockdowns led to the closure of most in-person pulmonary rehabilitation programs in Australia. Text message programs are effective for delivering health support to aid the self-management of people with chronic diseases. This study aimed to evaluate the implementation of a six-month pre-post text message support program (Texting for Wellness: Lung Support Service), and the enablers and barriers to its adoption and implementation. Methods: This mixed-methods pre-post study used the Reach, Effectiveness, Adoption, Implementation and Maintenance (RE-AIM) framework to evaluate the Texting for Wellness: Lung Support Service, which is an automated six-month text message support program that included evidence-based lifestyle, disease-self management and COVID-19-related information. Reach was measured by the proportion of participant enrolments and demographic characteristics. Adoption enablers and barriers were measured using text message response data and a user feedback survey (five-point Likert scale questions and free-text responses). Implementation was evaluated to determine fidelity including text message delivery data, opt-outs, and intervention costs to promote and deliver the program. Results: In total, 707/1940 (36.4%) participants enrolled and provided e-consent, with a mean age (±standard deviation) of 67.9 (±9.2) years old (range: 23–87 years). Of participants who provided feedback, (326/707) most ‘agreed’ or ‘strongly agreed’ that the text messages were easy to understand (98.5%), helpful them to feel supported (92.3%) and helped them to manage their health (88.0%). Factors influencing engagement included a feeling of support and reducing loneliness, and its usefulness for health self-management. Messages were delivered as planned (93.7% successfully delivered) with minimal participant dropouts (92.2% retention rate) and low cost ($AUD24.48/participant for six months). A total of 2263 text message replies were received from 496 unique participants. There were no reported adverse events. Conclusion: Texting for Wellness: Lung Support Service was implemented quickly, had a broad reach, with high retention and acceptability among participants. The program was low cost and required minimal staff oversight, which may facilitate future implementation. Further research is needed to evaluate the efficacy of text messaging for the improvement of lung health outcomes and strategies for long-term pulmonary rehabilitation program maintenance.
Infectivity of SARS-CoV-2 on Inanimate Surfaces: Don’t Trust Ct Value
Authors: Knobloch Johannes K. , Pfefferle Susanne , Lütgehetmann Marc , Nörz Dominik , Klupp Eva M. , Belmar Campos Cristina E. , Kluge Stefan , Aepfelbacher Martin , Knobling Birte , Franke Gefion
Published: December 2022

SARS-CoV-2 RNA is frequently identified in patient rooms and it was speculated that the viral load quantified by PCR might correlate with infectivity of surfaces. To evaluate Ct values for the prediction of infectivity, we investigated contaminated surfaces and Ct-value changes after disinfection. Viral RNA was detected on 37 of 143 investigated surfaces of an ICU. However, virus isolation failed for surfaces with a high viral RNA load. Also, SARS-CoV-2 could not be cultivated from surfaces artificially contaminated with patient specimens. In order to evaluate the significance of Ct values more precisely, we used surrogate enveloped bacteriophage Φ6. A strong reduction in Φ6 was achieved by three different disinfection methods. Despite a strong reduction in viability almost no change in the Ct values was observed for UV-C and alcoholic surface disinfectant. Disinfection using ozone resulted in a lack of Φ6 recovery as well as a detectable shift in Ct values indicating strong degradation of the viral RNA. The observed lack of significant effects on the detectable viral RNA after effective disinfection suggest that quantitative PCR is not suitable for predicting the infectivity of SARS-CoV-2 on inanimate surfaces. Ct values should therefore not be considered as markers for infectivity in this context.
Effectiveness of Infection Control Teams in Reducing Healthcare-Associated Infections: A Systematic Review and Meta-Analysis
Authors: Thandar Moe Moe , Rahman Md. Obaidur , Haruyama Rei , Matsuoka Sadatoshi , Okawa Sumiyo , Moriyama Jun , Yokobori Yuta , Matsubara Chieko , Nagai Mari , Ota Erika , Baba Toshiaki
Published: December 2022

The infection control team (ICT) ensures the implementation of infection control guidelines in healthcare facilities. This systematic review aims to evaluate the effectiveness of ICT, with or without an infection control link nurse (ICLN) system, in reducing healthcare-associated infections (HCAIs). We searched four databases to identify randomised controlled trials (RCTs) in inpatient, outpatient and long-term care facilities. We judged the quality of the studies, conducted meta-analyses whenever interventions and outcome measures were comparable in at least two studies, and assessed the certainty of evidence. Nine RCTs were included; all were rated as being low quality. Overall, ICT, with or without an ICLN system, did not reduce the incidence rate of HCAIs [risk ratio (RR) = 0.65, 95% confidence interval (CI): 0.45–1.07], death due to HCAIs (RR = 0.32, 95% CI: 0.04–2.69) and length of hospital stay (42 days vs. 45 days, p = 0.52). However, ICT with an ICLN system improved nurses’ compliance with infection control practices (RR = 1.17, 95% CI: 1.00–1.38). Due to the high level of bias, inconsistency and imprecision, these findings should be considered with caution. High-quality studies using similar outcome measures are needed to demonstrate the effectiveness and cost-effectiveness of ICT.
Association between Physical Activity Levels and Brain Volumes in Adults Visiting Radio-Imaging Center of Tertiary Care Hospital
Authors: Raja Deepika , Ravichandran Sneha , Chandrasekaran Baskaran , Kadavigere Rajagopal , Babu M. G. Ramesh , Almeshari Meshari , Alyahyawi Amjad R. , Alzamil Yasser , Abanomy Ahmad , Sukumar Suresh
Published: December 2022

Background and aim: There is evidence to support the favorable impact of physical activity (PA) on brain volume. However, the empirical evidence exploring the relationship between physical and sedentary behavior remains mixed. We aimed to explore the relationship between PA and sedentary behavior and brain volume. Methods: The study sample (n = 150, mean age = 39.7 years) included patients interviewed with the International Physical Activity Questionnaire (IPAQ) who underwent an MRI brain scan. From the images obtained, we measured total intracranial, gray matter, and white matter volume along with the hippocampus, amygdala, parahippocampal gyrus, and posterior cingulate cortex (PCC). Multivariable linear regression analysis was done. Results and discussion: Left hippocampus and overall PA were positively and significantly associated (β = 0.71, p = 0.021) whereas time spent on vigorous physical activity showed a negative association (β = −0.328, p = 0.049) with left hippocampal volume. Conclusion: We found a positive association between total PA and the left hippocampus, whereas vigorous PA showed a negative association with the left hippocampus.
Experiences of Nursing Instructors Related to Safety Issues Using Students as Practice Models in Laboratories: A Focus Group Study
Authors: Kang Youngmi , Choi Dongwon , Park Soohyun
Published: December 2022

Aim: Nursing school students perform invasive (i.e., injection, venipuncture) and/or non-invasive procedures (i.e., giving a bed bath and back massage) on each other to master these skills, and nursing instructors reported related safety issues. This study aimed to explore nursing instructors’ experiences concerning their students’ psychological and physical safety when using students as practice models in nursing skills laboratories. Methods: A qualitative design using focus group interviews and thematic analysis was employed. Two semi-structured focus group interviews were conducted with a purposive sample of eight instructors with experience in teaching nursing skills in laboratories. This study was evaluated by the Institutional Review Board at Eulji University (EU18-51) in the Republic of Korea. Results: Three main themes emerged to describe nursing instructors’ safety-related experiences when using students as practice models in nursing skills laboratories: (1) a dilemma between the experimental learning of students and the need to keep students safe, (2) perception related to psychological safety, and (3) an inadequate safety reporting system. Conclusions: When instructors consider using students’ bodies to practice nursing skills, they experience a dilemma between the students’ experimental learning and the need to keep them safe. Thus, methods to maximize student learning and student safety guidelines should be developed.
Morphological Pattern of Building Clusters in Cold Regions: Evidence from Harbin
Authors: Pan Wente , Li Shuqi , Ye Yang , Huang Yuan , Liu Haocheng , Liu Hongxing , Yu Wenxuan
Published: December 2022

The rapidly changing global conditions of the environment and climate have resulted in higher requirements for urban design. Significant annual temperature variations and large day/night temperature differences in cold-region cities leads to high energy consumption. Therefore, it is challenging to achieve low energy consumption in cold-region cities. Urban morphology focuses on the physical elements of urban areas, reflecting the relationship between the city and its environment and the city’s response to natural climatic conditions. Building clusters are common in cold regions due to the extreme climate. Thus, it is crucial to study the energy performance of cities by considering urban morphology. This study focuses on four morphological patterns of building clusters: point, linear, courtyard, and mixed patterns. A case study is conducted in Harbin, a cold-region city in China. Samples of the four morphological patterns are extracted, and GIS analysis and manual labeling are used to analyze the dominant morphological patterns of building clusters in cold regions. Average nearest-neighbor analysis is used to obtain quantitative results and determine the prevalence of different morphological patterns of building clusters in cold regions. This process can be used to determine the dominant patterns of urban building clusters and provide a scientific basis for selecting the morphological patterns of new building clusters in cold regions.
Comparison of Typical Alpine Lake Surface Elevation Variations and Different Driving Forces by Remote Sensing Altimetry Method
Authors: Pan Yaming , Du Weibing , Ma Dandan , Lyu Xiaoxuan , Cheng Chaoying
Published: December 2022

Alpine lakes play a significant role in improving watershed ecology, adjusting water storage, and managing regional water resources. They are also a valuable freshwater reservoir, flood storage, and species gene pool in Central Asia. This article validated the accuracy of the CryoSat-2 footprints altimetry dataset for the Lake Bosten and Lake Issyk-Kul ranges. The time series for the surface elevations of the Central Asian alpine lakes Karakul and Chatyrkul were established, based on footprints altimetry data. The lake hydrological drivers were analyzed using remote sensing meteorological reanalysis data of the lake basins. The following main conclusions were reached. The CryoSat-2 footprints altimetry dataset has high confidence in lake surface elevation monitoring. Compared with Hydroweb monitoring results, the agreement between the monitoring results in the range between Lake Bosten and Lake Issyk-Kul are 0.96 and 0.84. The surface elevation of Lake Karakul shows an overall increasing trend with a variation rate of +7.7 cm/yr from 2010 to 2020, which has a positive correlation with the temperature in the basin. This indicates that the increased temperature, which results in the increased snow and ice meltwater in the basin, is the main driving force of the increased lake evolution. The lake surface elevation of Lake Chatyrkul shows an overall decreasing trend, with a variation rate of −9.9 cm/yr from 2010 to 2020, which has a negative correlation with the temperature in the basin. This suggests that Lake Chatyrkul is poorly recharged by snow and ice meltwater. The main driving force of its evolution is the increased evaporative output of the lake due to the increase in temperature. These conclusions prove that temperature and alpine glacial variability within the lake basin play an important role in lake surface elevation variations in alpine regions of Central Asia.
Status and Prospect of Ecological Environment in the Belt and Road Initiative Regions
Authors: Du Xixi , Qin Yi , Huang Chunbo
Published: December 2022

With the widespread recognition and in-depth implementation of the Belt and Road Initiative (BRI), especially in the context of global climate change, the ecological environment of Belt and Road Initiative regions might be confronted with pressures and challenges with rapid socioeconomic development. In response to those potential environmental challenges, China has put forward Green BRI and enriched the new Silk Road with more environmental connotations, aiming to reduce the conflict between economic development and eco-environmental protection. Currently, there is a lack of systematic and holistic research on eco-environmental issues in BRI regions. In addition, feasible solutions to enhance BRI’s contribution to the eco-environment remain insufficient. Having systematically reviewed the relevant literature on the eco-environment in BRI regions, we found that most regions along the BRI routes are in sensitive zones of climate and geological change, with fragile eco-environments and strong vulnerability to climate change, natural disasters and human activities. The main eco-environment status of the BRI regions is as follows: (1) The total water resources in BRI regions account for only 36% of the global total, with uneven distribution and complex spatial precipitation, posing higher pressure on water security. (2) Vegetation varies significantly from region to region. The vegetation in South Asia is the richest, with its mean annual NDVI exceeding 0.7. The NDVI in East Europe, Russia and South China are between 0.4 and 0.7, and that in Central Asia and West Asia are below 0.2. (3) The BRI regions are abundantly blessed with natural resources, with the total recoverable oil reserves, natural gas reserves and the total mining area reaching 66%, 65.5% and 42.31% of the world’s total, respectively, but severe overexploitation and overconsumption of those resources degrade their eco-environment. Accordingly, future research directions, such as target on integrated, interdisciplinary and coordinated studies on eco-environmental issues in BRI regions, are proposed in this paper to achieve optimization of BRI’s contribution to eco-environment protection in BRI regions.
Mercury Sources, Emissions, Distribution and Bioavailability along an Estuarine Gradient under Semiarid Conditions in Northeast Brazil
Authors: Moura Victor Lacerda , de Lacerda Luiz Drude
Published: December 2022

In the semiarid coast of northeast Brazil, climate change and changes in land use in drainage basins affect river hydrodynamics and hydrochemistry, modifying the estuarine environment and its biogeochemistry and increasing the mobilization of mercury (Hg). This is particularly relevant to the largest semiarid-encroached basin of the region, the Jaguaribe River. Major Hg sources to the Jaguaribe estuary are solid waste disposal, sewage and shrimp farming, the latter emitting effluents directly into the estuary. Total annual emission reaches 300 kg. In that estuary, the distribution of Hg in sediment and suspended particulate matter decreases seaward, whereas dissolved Hg concentrations increase sharply seaward, suggesting higher mobilization at the marine-influenced, mangrove-dominated portion of the estuary, mostly in the dry season. Concentrations of Hg in rooted macrophytes respond to Hg concentrations in sediment, being higher in the fluvial endmember of the estuary, whereas in floating aquatic macrophytes, Hg concentrations followed dissolved Hg concentrations in water and were also higher in the dry season. Animals (fish and crustaceans) also showed higher concentrations and bioaccumulation in the marine-influenced portion of the estuary. The variability of Hg concentrations in plants and sediments agrees with continental sources of Hg. However, Hg fractionation in water and contents in the animals respond to higher Hg availability in the marine-dominated end of the estuary. The results suggest that the impact of anthropogenic sources on Hg bioavailability is modulated by regional and global environmental changes and results from a conjunction of biological, ecological and hydrological characteristics. Finally, increasing aridity due to global warming, observed in northeast Brazil, as well as in other semiarid littorals worldwide, in addition to increased water overuse, augment Hg bioavailability and environmental risk and exposure of the local biota and the tradition of human populations exploiting the estuary’s biological resources.
From Low-Grade Inflammation in Osteoarthritis to Neuropsychiatric Sequelae: A Narrative Review
Authors: Naumovs Vladimirs , Groma Valērija , Mednieks Jānis
Published: December 2022

Nowadays, osteoarthritis (OA), a common, multifactorial musculoskeletal disease, is considered to have a low-grade inflammatory pathogenetic component. Lately, neuropsychiatric sequelae of the disease have gained recognition. However, a link between the peripheral inflammatory process of OA and the development of neuropsychiatric pathology is not completely understood. In this review, we provide a narrative that explores the development of neuropsychiatric disease in the presence of chronic peripheral low-grade inflammation with a focus on its signaling to the brain. We describe the development of a pro-inflammatory environment in the OA-affected joint. We discuss inflammation-signaling pathways that link the affected joint to the central nervous system, mainly using primary sensory afferents and blood circulation via circumventricular organs and cerebral endothelium. The review describes molecular and cellular changes in the brain, recognized in the presence of chronic peripheral inflammation. In addition, changes in the volume of gray matter and alterations of connectivity important for the assessment of the efficacy of treatment in OA are discussed in the given review. Finally, the narrative considers the importance of the use of neuropsychiatric diagnostic tools for a disease with an inflammatory component in the clinical setting.
The Aging Heart: A Molecular and Clinical Challenge
Authors: Lazzeroni Davide , Villatore Andrea , Souryal Gaia , Pili Gianluca , Peretto Giovanni
Published: December 2022

Aging is associated with an increasing burden of morbidity, especially for cardiovascular diseases (CVDs). General cardiovascular risk factors, ischemic heart diseases, heart failure, arrhythmias, and cardiomyopathies present a significant prevalence in older people, and are characterized by peculiar clinical manifestations that have distinct features compared with the same conditions in a younger population. Remarkably, the aging heart phenotype in both healthy individuals and patients with CVD reflects modifications at the cellular level. An improvement in the knowledge of the physiological and pathological molecular mechanisms underlying cardiac aging could improve clinical management of older patients and offer new therapeutic targets.
Inhibition of TIGAR Increases Exogenous p53 and Cisplatin Combination Sensitivity in Lung Cancer Cells by Regulating Glycolytic Flux
Authors: Fu Jiaying , Yu Sihang , Zhao Xiyao , Zhang Chaoke , Shen Luyan , Liu Yanan , Yu Huimei
Published: December 2022

The metabolism and apoptosis of tumor cells are important factors that increase their sensitivity to chemotherapeutic drugs. p53 and cisplatin not only induce tumor cell apoptosis, but also regulate the tumor cell metabolism. The TP53-induced glycolysis and apoptosis regulator (TIGAR) can inhibit glycolysis and promote more glucose metabolism in the pentose phosphate pathway. We speculate that the regulation of the TIGAR by the combination therapy of p53 and cisplatin plays an important role in increasing the sensitivity of tumor cells to cisplatin. In this study, we found that the combined treatment of p53 and cisplatin was able to inhibit the mitochondrial function, promote mitochondrial pathway-induced apoptosis, and increase the sensitivity. Furthermore, the expression of the TIGAR was inhibited after a combined p53 and cisplatin treatment, the features of the TIGAR that regulate the pentose phosphate pathway were inhibited, the glucose flux shifted towards glycolysis, and the localization of the complex of the TIGAR and Hexokinase 2 (HK2) on the mitochondria was also reduced. Therefore, the combined treatment of p53 and cisplatin may modulate a glycolytic flux through the TIGAR, altering the cellular metabolic patterns while increasing apoptosis. Taken together, our findings reveal that the TIGAR may serve as a potential therapeutic target to increase the sensitivity of lung cancer A549 cells to cisplatin.
The Molecular Mechanisms and Function of miR-15a/16 Dysregulation in Fibrotic Diseases
Authors: Wen Dada , Zhang Huamin , Zhou Yutong , Wang Jie
Published: December 2022

MicroRNAs (miRNAs) are a class of short, endogenous, non-coding, single-stranded RNAs that can negatively regulate the post-transcriptional expression of target genes. Among them, miR-15a/16 is involved in the regulation of the occurrence and development of fibrosis in the liver, lungs, heart, kidneys, and other organs, as well as systemic fibrotic diseases, affecting important cellular functions, such as cell transformation, the synthesis and degradation of extracellular matrix, and the release of fibrotic mediators. Therefore, this article reviews the biological characteristics of miR-15a/16 and the molecular mechanisms and functions of their dysregulation in fibrotic diseases.
The Arylamidine T-2307 as a Novel Treatment for the Prevention and Eradication of Candida tropicalis Biofilms
Authors: Maione Angela , La Pietra Alessandra , Siciliano Antonietta , Mileo Aldo , De Falco Maria , de Alteriis Elisabetta , Guida Marco , Galdiero Emilia
Published: December 2022

Candida tropicalis is an emerging pathogen with a high mortality rate due to its virulence factors, including biofilm formation, that has important repercussions on the public health system. The ability of C. tropicalis to form biofilms, which are potentially more resistant to antifungal drugs and the consequent increasing antimicrobial resistance, highlights an urgent need for the development of novel antifungal. The present study analyzed the antibiofilm capacity of the arylamidine T-2307 on two strains of Candida tropicalis. Antimicrobial activity and time-killing assays were performed to evaluate the anticandidal effects of T-2307, the antibiofilm ability on biomass inhibition and eradication was evaluated by the crystal violet (CV) method. Furthermore, in Galleria mellonella infected larvae an increased survival after pre—and post- treatment with T-2307 was observed. The MTT test was used to determine the viability of immortalized human prostate epithelial cells (PNT1A) after exposure to different concentrations of T-2307. Levels of interleukin IL-4, IL-8, IL-10 were quantified after Candida infection of PNT1A cells and treatment. Active doses of T-2307 did not affect the viability of PNT1A cells, and drug concentrations of 0.005 or 0.01 µg mL−1 inhibited the secretion of inflammatory cytokines. Taken together, these results provide new information on T-2307, indicating this drug as a new and promising alternative therapeutic option for the treatment of Candida infections.
Solubility and Thermal Stability of Thermotoga maritima MreB
Authors: Longauer Beáta , Bódis Emőke , Lukács András , Barkó Szilvia , Nyitrai Miklós
Published: December 2022

The basis of MreB research is the study of the MreB protein from the Thermotoga maritima species, since it was the first one whose crystal structure was described. Since MreB proteins from different bacterial species show different polymerisation properties in terms of nucleotide and salt dependence, we conducted our research in this direction. For this, we performed measurements based on tryptophan emission, which were supplemented with temperature-dependent and chemical denaturation experiments. The role of nucleotide binding was studied through the fluorescent analogue TNP-ATP. These experiments show that Thermotoga maritima MreB is stabilised in the presence of low salt buffer and ATP. In the course of our work, we developed a new expression and purification procedure that allows us to obtain a large amount of pure, functional protein.
Activating Wnt/β-Catenin Signaling in Osteocytes Promotes Osteogenic Differentiation of BMSCs through BMP-7
Authors: Zhang Yining , Zhao Yixin , Xie Zhengsong , Li Molin , Liu Yujiao , Tu Xiaolin
Published: December 2022

Bone formation is critically needed in orthopedic clinical practice. We found that, bone morphogenetic protein-7 (BMP-7) gene expression was significantly increased in fractured mice, which activates canonical Wnt signaling exclusively in osteocytes. Wnt and BMP signaling appear to exhibit synergistic or antagonistic effects in different kinds of cells. However, the communication between Wnt/β-catenin signaling and BMP signaling in osteocytes is almost unknown. Our study verified in vitro that BMP-7 expression was significantly increased when Wnt signaling was activated in osteocytes. Next, BMP-7 in osteocytes was overexpressed using an adenovirus, the osteogenesis of bone marrow stem cells (BMSCs) was enhanced, when cocultured with osteocytes. On the contrary, BMP-7 in osteocytes was silenced using an adenovirus, the osteogenesis of bone marrow stem cells (BMSCs) was weakened. In addition, the osteogenesis of BMSCs was no longer promoted by Wnt-activated osteocytes when BMP-7 was silenced. Therefore, the results showed that BMP-7 mediated the anabolic actions of Wnt/β-catenin signaling in osteocytes. Our study provides new evidence for the clinical application of BMP-7-overexpressed osteocytes.
Genome-Wide Identification of the SUN Gene Family in Melon (Cucumis melo) and Functional Characterization of Two CmSUN Genes in Regulating Fruit Shape Variation
Authors: Ma Ming , Liu Suya , Wang Zhiwei , Shao Ran , Ye Jianrong , Yan Wei , Lv Hailing , Hasi Agula , Che Gen
Published: December 2022

Melon (Cucumis melo) is an important economic crop cultivated worldwide. A unique SUN gene family plays a crucial role in regulating plant growth and fruit development, but many SUN family genes and their function have not been well-characterized in melon. In the present study, we performed genome-wide identification and bioinformatics analysis and identified 24 CmSUN family genes that contain integrated and conserved IQ67 domain in the melon genome. Transcriptome data analysis and qRT-PCR results showed that most CmSUNs are specifically enriched in melon reproductive organs, such as young flowers and ovaries. Through genetic transformation in melons, we found that overexpression of CmSUN23-24 and CmSUN25-26-27c led to an increased fruit shape index, suggesting that they act as essential regulators in melon fruit shape variation. Subcellular localization revealed that the CmSUN23-24 protein is located in the cytoplasmic membrane. A direct interaction between CmSUN23-24 and a Calmodulin protein CmCaM5 was found by yeast two-hybrid assay, which indicated their participation in the calcium signal transduction pathway in regulating plant growth. These findings revealed the molecular characteristics, expression profile, and functional pattern of the CmSUN genes, and may provide the theoretical basis for the genetic improvement of melon fruit breeding.
Intravesicular Genomic DNA Enriched by Size Exclusion Chromatography Can Enhance Lung Cancer Oncogene Mutation Detection Sensitivity
Authors: Van Hoof Rebekka , Deville Sarah , Hollanders Karen , Berckmans Pascale , Wagner Patrick , Hooyberghs Jef , Nelissen Inge
Published: December 2022

Extracellular vesicles (EVs) are cell-derived structures surrounded by a lipid bilayer that carry RNA and DNA as potential templates for molecular diagnostics, e.g., in cancer genotyping. While it has been established that DNA templates appear on the outside of EVs, no consensus exists on which nucleic acid species inside small EVs (<200 nm, sEVs) are sufficiently abundant and accessible for developing genotyping protocols. We investigated this by extracting total intravesicular nucleic acid content from sEVs isolated from the conditioned cell medium of the human NCI-H1975 cell line containing the epidermal growth factor (EGFR) gene mutation T790M as a model system for non-small cell lung cancer. We observed that mainly short genomic DNA (<35–100 bp) present in the sEVs served as a template. Using qEV size exclusion chromatography (SEC), significantly lower yield and higher purity of isolated sEV fractions were obtained as compared to exoEasy membrane affinity purification and ultracentrifugation. Nevertheless, we detected the EGFR T790M mutation in the sEVs’ lumen with similar sensitivity using digital PCR. When applying SEC-based sEV separation prior to cell-free DNA extraction on spiked human plasma samples, we found significantly higher mutant allele frequencies as compared to standard cell-free DNA extraction, which in part was due to co-purification of circulating tumor DNA. We conclude that intravesicular genomic DNA can be exploited next to ctDNA to enhance EGFR T790M mutation detection sensitivity by adding a fast and easy-to-use sEV separation method, such as SEC, upstream of standard clinical cell-free DNA workflows.
Mitochondrial Dysfunction and Therapeutic Perspectives in Cardiovascular Diseases
Authors: Liu Yu , Huang Yuejia , Xu Chong , An Peng , Luo Yongting , Jiao Lei , Luo Junjie , Li Yongzhi
Published: December 2022

High mortality rates due to cardiovascular diseases (CVDs) have attracted worldwide attention. It has been reported that mitochondrial dysfunction is one of the most important mechanisms affecting the pathogenesis of CVDs. Mitochondrial DNA (mtDNA) mutations may result in impaired oxidative phosphorylation (OXPHOS), abnormal respiratory chains, and ATP production. In dysfunctional mitochondria, the electron transport chain (ETC) is uncoupled and the energy supply is reduced, while reactive oxygen species (ROS) production is increased. Here, we discussed and analyzed the relationship between mtDNA mutations, impaired mitophagy, decreased OXPHOS, elevated ROS, and CVDs from the perspective of mitochondrial dysfunction. Furthermore, we explored current potential therapeutic strategies for CVDs by eliminating mtDNA mutations (e.g., mtDNA editing and mitochondrial replacement), enhancing mitophagy, improving OXPHOS capacity (e.g., supplement with NAD+, nicotinamide riboside (NR), nicotinamide mononucleotide (NMN), and nano-drug delivery), and reducing ROS (e.g., supplement with Coenzyme Q10 and other antioxidants), and dissected their respective advantages and limitations. In fact, some therapeutic strategies are still a long way from achieving safe and effective clinical treatment. Although establishing effective and safe therapeutic strategies for CVDs remains challenging, starting from a mitochondrial perspective holds bright prospects.