bluuebunny/crossref_metadata_2025 huggingface dataset:
Building a Predictive Model of Social-Emotional Adjustment: Exploring the Relationship between Parenting Self-Efficacy, Parenting Behaviour and Psychological Distress in Mothers of Young Children in Ireland
Authors: Coyle Sabrina , Sarma Kiran M. , Maguire Catherine , De Flumere Leora
Published: March 2021

The purpose of this study was to generate greater understanding of social-emotional difficulties in infants and toddlers in an Irish context. This study compared rates of reported social-emotional difficulties in young children in clinical and non-clinical samples and probed a predictive model of social-emotional adjustment. Data were collected from a cross-sectional sample of 72 mothers of young children aged between 12 and 48 months. Mothers were recruited from waiting lists for child Early Intervention services (clinical sample) and community mother-toddler groups (non-clinical sample). Mothers completed a questionnaire battery which assessed parenting self-efficacy, parenting behaviour, psychological distress and child social-emotional adjustment. The results indicated that 55.5% of young children in the clinical sample and 15% in the non-clinical sample had significant social-emotional problems. Similarly, 55.5% of young children in the clinical sample and 30% in the non-clinical sample had significant delays in the acquisition of social-emotional competencies. Two hierarchical multiple regressions were carried out with social-emotional problems and social-emotional competencies as the respective criterion variables. Clinical or non-clinical group membership, parenting satisfaction and maternal psychological distress were found to be significant predictors of child social-emotional problems in a model which explained 59% of the variance. Task-specific self-efficacy was the only significant predictor of child social-emotional competencies in a model which explained 21% of the variance. The significant rates of social-emotional problems in young children in the current study and the potential negative impact on child health and wellbeing, suggest that the early assessment of social-emotional adjustment should be incorporated into routine clinical assessment for young children. For services to effectively meet the needs of children with social-emotional difficulties and their families, consideration of maternal factors is also necessary.
The Relationship between Long Noncoding RNA H19 Polymorphism and the Epidermal Growth Factor Receptor Phenotypes on the Clinicopathological Characteristics of Lung Adenocarcinoma
Authors: Wang Yao-Chen , Tsao Shih-Ming , Li Yia-Ting , Lee Chia-Yi , Tsao Thomas Chang-Yao , Hsieh Ming-Ju , Yang Shun-Fa
Published: March 2021

The aim of the current study is to investigate potential associations among Long Noncoding RNA (LncRNA) H19 single nucleotide polymorphism (SNP) and epidermal growth factor receptor (EGFR) phenotypes on the clinicopathological characteristics of lung adenocarcinoma (LADC). Five loci of LncRNA H19 SNPs (rs217727, rs2107425, rs2839698, rs3024270, and rs3741219) were genotyped by using TaqMan allelic discrimination in 223 LADC patients with wild-type EGFR phenotype and 323 LADC individuals with EGFR mutations. After the statistical analyses, patients with the EGFR mutation were related to a higher distribution frequency of rs217727 SNP CT heterozygote (p = 0.030), and the female population with EGFR mutation demonstrated a higher distribution frequency of rs217727 SNP CT heterozygote (p < 0.001) and rs2107425 CT heterozygote (p = 0.002). In addition, the presence of LncRNA H19 SNP rs217727 T allele (CT + TT) in patients with EGFR wild-type was associated to higher tumor T status (stage III or IV, p = 0.037) and poorer cell differentiation status (poor differentiation, p = 0.012) compared to those EGFR wild-type individuals with LncRNA H19 SNP rs217727 CC allele. Besides, a prominently higher tumor T status was found in subjects with LncRNA H19 SNP rs2107425 T allele (CT + TT) (stage III or IV, p = 0.007) compared to EGFR wild-type LADC individuals with LncRNA CC allele in EGFR wild-type patients. Our findings suggest that the presence of LncRNA H19 SNP rs217727 is related to the EGFR mutation in LADC patients, and the LncRNA H19 SNP rs217727 and rs2107425 are associated with progressed tumor status for LADC patients with EGFR wild-type.
An Interaction Path of Mothers’ and Preschoolers’ Food- and Physical Activity-Related Aspects in Disadvantaged Sicilian Urban Areas
Authors: Tabacchi Garden , Petrigna Luca , Battaglia Giuseppe , Navarra Giovanni , Palma Antonio , Bellafiore Marianna
Published: March 2021

Background: The relationship between mothers and their children’s lifestyle is still unclear, especially in disadvantaged areas. Consequently, the study aims to identify a path explaining the extent to which maternal eating habits and physical activity (PA) level predict food-related aspects, PA practice and Quotient of Gross Motor Development (QGMD) in preschoolers from disadvantaged urban areas. Methods: In this cross-sectional study, a total of 79 dyads of mothers and children were recruited from kindergartens. Information related to family socio-demographic aspects, mothers’ and children’s dietary intake frequencies and PA/sedentariness, mothers’ weight and height, mothers’ perception on children’s food intake, and children’s food literacy (FL) was collected with a questionnaire and the Food Literacy Assessment Tool (preschool-FLAT), while gross-motor skills were measured with the Test of Gross Motor Development (TGMD); weight and height of children were directly collected. Results: Associations were found between mothers’ and children’s food habits; mothers’ and children’s fruit/vegetables consumption, and intake of the other items; mothers’ education or PA level and children’s FL; mothers’ PA or sedentariness and children’s QGMD; mothers’ BMI and food habits and children’s BMI; education and food habits. Conclusions: These findings can be useful to plan effective interventions targeted both to preschoolers and their mothers of disadvantaged urban areas for promoting healthy lifestyles, which have become increasingly difficult to achieve during COVID-19 pandemic.
The Effect of a Non-Invasive Positive Pressure Ventilation Simulation Program on General Ward Nurses’ Knowledge and Self-Efficacy
Authors: Kim Moon-Sook , Seo Mi-Hee , Jung Jin-Young , Kim Jinhyun
Published: March 2021

The purpose of this study is to develop a simulation-based ventilator training program for general ward nurses and identify its effects. Quantitative data were collected from 29 nurses (intervention group: 15, control group: 14), of which seven were interviewed with focus groups to collect qualitative data. The quantitative results revealed significant differences in ventilator-related knowledge (p = 0.029) and self-efficacy (p = 0.026) between the intervention and control groups. Moreover, three themes were derived from meaningful statements in the qualitative data: understanding psychophysical discomfort of the patient while applying the ventilator; helping in ventilator care; and establishing a future ventilator training strategy. The findings confirmed that the non-invasive positive pressure ventilation (NPPV) simulation program is an effective method for improving the knowledge of ventilator nursing and self-efficacy and will be helpful in developing educational methods and strategies related to ventilator nursing for general ward nurses.
Class of 2020 in Poland: Students’ Mental Health during the COVID-19 Outbreak in an Academic Setting
Authors: Wieczorek Tomasz , Kołodziejczyk Agata , Ciułkowicz Marta , Maciaszek Julian , Misiak Błażej , Rymaszewska Joanna , Szcześniak Dorota
Published: March 2021

The COVID-19 pandemic had led universities to introduce lockdowns, which has led to significant shifts in students’ lives. Classes were moved online, students had to leave dorms and move; they had to forgo regular meetings with their peers. Subsequently, a vital demand for examining students’ mental health emerged. The data were collected at a time when universities in Poland were under lockdowns. Participants represented students of many different fields of study. The General Health Questionnaire (GHQ-28) was used. Student’s subjective evaluation of online learning and their adaptation to academic life shifts were reviewed. A total of 1123 participants took part in this study. Relationship analysis included tests, such as U Mann–Whitney, appropriate for specific variables. The impact of variables connected with the pandemic on the GHQ scores was tested using multivariate regression analysis. The results were considered significant at a p-value set at 0.05. Overall, 76.96% of the participants manifested psychopathological symptoms measured by the GHQ. Four demographical variables were possibly associated with the GHQ scores: female sex, living in a big city, necessity to move back home, and being in a relationship. Negative correlations between subjective evaluation of variables concerning e-learning, such as its efficiency or quality, and the GHQ scores were found. Some variables were found to be potential protective factors, whereas others could have contributed to worsened mental health. The study provides data on students’ worsened mental health due to the COVID-19 pandemic and the shifts in academic life it caused. Therefore, recommendations for early psychosocial interventions among students are strongly advised.
Gender Specificity of Spousal Concordance in the Development of Chronic Disease among Middle-Aged and Older Chinese Couples: A Prospective Dyadic Analysis
Authors: Liao Jing , Zhang Jing , Xie Jinzhao , Gu Jing
Published: March 2021

This study aimed to explore the gender specificity of spousal concordance in the development of chronic diseases among middle-aged and older Chinese couples. Data of 3420 couples were obtained from the China Health and Retirement Longitudinal Study (CHARLS). Multivariate logistic regression was used to analyze the incidence of chronic disease development over 4 years, conditional on the spousal baseline chronic disease status; and stepwise adjusting for the couples’ sociodemographic characteristics (i.e., age, education, retirement status and household income), and their individual lifestyle (i.e., smoking, drinking, exercise, social participation and BMI) all measured at baseline. The incidence of chronic diseases after 4 years of follow-up was 22.95% in the husbands (605/2636) and 24.71% in the wives (623/2521). Taking the couples’ baseline sociodemographic and lifestyle covariates into account, husbands whose wife had chronic diseases at baseline showed an increased risk of developing chronic diseases over 4 years (ORadjusted = 1.24, 95%CI: 1.02, 1.51), but this risk was not statistically-significant for wives (ORadjusted = 0.88, 95%CI: 0.71, 1.08). Our study identified gender specificity of spousal concordance in the development of chronic diseases among middle-aged and older Chinese couples. This finding may contribute to the design of couple-based intervention for disease prevention and management for community-dwelling older adults.
A Qualitative Account of Young People’s Experiences Seeking Care from Emergency Departments for Self-Harm
Authors: Byrne Sadhbh J. , Bellairs-Walsh India , Rice Simon M. , Bendall Sarah , Lamblin Michelle , Boubis Emily , McGregor Brianna , O’Keefe Meghan , Robinson Jo
Published: March 2021

Many young people who engage in self-harm do not seek help from health services. For those that do, emergency departments (EDs) are a key point of contact. Substantial gaps remain in current knowledge related to young consumers’ experiences and views on optimal treatment of self-harm in the ED. In this study, semi-structured interviews were conducted with thirteen young people (Mage = 21.2 years), who were engaged with care at headspace early intervention centers and had presented to an ED with a self-inflicted physical injury. Participants were asked to describe their experience in the ED and the care they received. Data were analyzed thematically. Three inter-related themes were identified: 1. The ED was experienced through a lens of significant distress, 2. The ED environment and processes were counter-therapeutic, and 3. Staff were perceived to be disinterested, dismissive, and lacking in knowledge. The study highlights the overwhelmingly negative nature of participants’ experiences, and presents recommendations for service and practice improvements, such as the provision of staff training and increased aftercare.
Meaning in Life Mediates the Association between Environmental Engagement and Loneliness
Authors: Jia Fanli , Soucie Kendall , Matsuba Kyle , Pratt Michael W.
Published: March 2021

Although the positive outcomes of human–environment interactions have been established, research examining the motivation between engagement in pro-environmental activities and psychological well-being is limited. In this mixed-methods study, the relationship between pro-environmental engagement, meaning in life, and well-being, including loneliness and depression, were investigated in a sample of 112 young adults in Canada. It was found that engaging in pro-environmental activities was negatively associated with loneliness. This association was mediated by meaning in life (e.g., an intrinsic motive of caring for future generations). In addition, qualitative analyses explored how engaging in pro-environmental activities has a meaningful impact on meaning in life, and on well-being. A thematic analysis generated three unique themes: (1) responsibility to teach the next generation about the environment, (2) deep appreciation for and connection to nature, and (3) renewed agency through self-directed learning. Overall, findings suggest that meaning in life is a core motive that underlies the association between environmental engagement and loneliness. The present study enriched the relationship between pro-environmentalism and well-being with a mixed-methods perspective.
The Effects of Age and Body Fat Content on Post-Downhill Run Recovery Following Whole Body Cryotherapy
Authors: Haq Adnan , Ribbans William , Baross Anthony W.
Published: March 2021

This study explored the effects of age and body fat content on responses to whole body cryotherapy (WBC) following a downhill running bout. Forty-one male participants (mean ± SD age 42.0 ± 13.7 years, body mass 75.2 ± 10.8 kg) were allocated into WBC (n = 26) and control (CON, n = 15) groups. WBC participants were divided into old (OLD, ≥45 years, n = 10) and young (YNG, <40 years, n = 13), as well as high fat (HFAT, ≥20%, n = 10) and low fat (LFAT ≤ 15%, n = 8) groups. Participants completed a 30 min downhill run (15% gradient) at 60% VO2 max. The WBC group underwent cryotherapy (3 min, −120 °C) 1 h post-run and CON participants passively recovered in a controlled environment (20 °C). Maximal isometric leg muscle torque was assessed pre and 24 h post-run. Blood creatine kinase (CK) and muscle soreness were assessed pre, post, one hour and 24 h post-run. Muscle torque significantly decreased in both groups post-downhill run (WBC: 220.6 ± 61.4 Nm vs. 208.3 ± 67.6 Nm, p = 0.02; CON: 239.7 ± 51.1 Nm vs. 212.1 ± 46.3 Nm, p = 0.00). The mean decrease in WBC was significantly less than in CON (p = 0.04). Soreness and CK increased 24 h post for WBC and CON (p < 0.01) with no difference between groups. Muscle torque significantly decreased in OLD participants (p = 0.04) but not in YNG (p = 0.55). There were no differences between HFAT and LFAT (all p values > 0.05). WBC may attenuate muscle damage and benefit muscle strength recovery following eccentrically biased exercises, particularly for young males.
Food Neophobia and Consumer Choices within Vietnamese Menu in a Polish Cohort Study
Authors: Guzek Dominika , Nguyen Duy , Głąbska Dominika
Published: March 2021

One of the factors influencing consumer food choices is food neophobia (FN), described as a reluctance to try novel or unknown food products. The aim of the study was to determine the influence of FN on food choices in young Polish respondents through a web-based choice experiment with Vietnamese restaurant menu. The choice experiment was conducted using a Computer-Assisted Web Interview (CAWI) method in a sample of 601 young adults, while using a developed Vietnamese restaurant menu. For the dishes, neophobic potential for a Polish population was defined, based on content of ingredients not typical for Polish diet. The FN was assessed using the Food Neophobia Scale (FNS) by Pliner and Hobden. The neophobic potential was the determinant of choice of dishes (p < 0.05). The participants characterized by a high FN level less commonly than others chosen dishes characterized by neophobic potential as: starter (Nem quõn—non-fried spring rolls with shrimps) (p = 0.0003), soup (Mién gà—soup with cellophane noodles and nam huong mushrooms) (p < 0.0001), main course (Phở xào bò—rice noodles with soy sauce and fish sauce) (p < 0.0001) and dessert (Chè thập cãm—dessert of golden gram, black eye beans, Azuki beans and tapioca) than other options (p = 0.0007). It was stated that FN in young respondents may reduce the frequency of choosing dishes containing unfamiliar ingredients and, as a result, it may cause lower diversity of consumed dishes. Taking into account that not properly balanced diets resulting from rejecting some types of products are becoming a growing problem, the FN should be taken into account in the general public health policy.
Indoor Model Simulation for COVID-19 Transport and Exposure
Authors: Hussein Tareq , Löndahl Jakob , Thuresson Sara , Alsved Malin , Al-Hunaiti Afnan , Saksela Kalle , Aqel Hazem , Junninen Heikki , Mahura Alexander , Kulmala Markku
Published: March 2021

Transmission of respiratory viruses is a complex process involving emission, deposition in the airways, and infection. Inhalation is often the most relevant transmission mode in indoor environments. For severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the risk of inhalation transmission is not yet fully understood. Here, we used an indoor aerosol model combined with a regional inhaled deposited dose model to examine the indoor transport of aerosols from an infected person with novel coronavirus disease (COVID-19) to a susceptible person and assess the potential inhaled dose rate of particles. Two scenarios with different ventilation rates were compared, as well as adult female versus male recipients. Assuming a source strength of 10 viruses/s, in a tightly closed room with poor ventilation (0.5 h−1), the respiratory tract deposited dose rate was 140–350 and 100–260 inhaled viruses/hour for males and females; respectively. With ventilation at 3 h−1 the dose rate was only 30–90 viruses/hour. Correcting for the half-life of SARS-CoV-2 in air, these numbers are reduced by a factor of 1.2–2.2 for poorly ventilated rooms and 1.1–1.4 for well-ventilated rooms. Combined with future determinations of virus emission rates, the size distribution of aerosols containing the virus, and the infectious dose, these results could play an important role in understanding the full picture of potential inhalation transmission in indoor environments.
Nine Months into the COVID-19 Pandemic: A Longitudinal Study Showing Mental Health and Movement Behaviours Are Impaired in UK Students
Authors: Savage Matthew J. , Hennis Philip J. , Magistro Daniele , Donaldson James , Healy Laura C. , James Ruth M.
Published: March 2021

Initial studies indicated that student mental health was impaired during the early stages of the pandemic and that maintaining/improving physical activity gave some protection from mental illness. However, as the pandemic persists, these data may not reflect current circumstances and may have been confounded by exam stress. Methods: This study used an online survey to assess the changes in, and associations between, mental health and movement behaviours in 255 UK university students from before the COVID-19 pandemic (October 2019) to 9 months following the UK’s first confirmed case (October 2020). Changes in and associations between mental wellbeing, perceived stress, physical activity, and sedentary behaviour were assessed using a mixed model ANOVA; a multiple linear regression model determined the predictive value of variables associated with Δ mental wellbeing. Results: Mental wellbeing and physical activity decreased (45.2 to 42.3 (p < 0.001); 223 to 173 min/week (p < 0.001)), whereas perceived stress and time spent sedentary increased (19.8 to 22.8 (p < 0.001); 66.0 to 71.2 h/week (p = 0.036)). Δ perceived stress, Δ sedentary behaviour and university year accounted for 64.7%, 12.9%, and 10.1% of the variance in Δ mental wellbeing (p < 0.001; p = 0.006; p = 0.035). Conclusion: The COVID-19 pandemic is having a sustained negative impact on student mental health and movement behaviour.
Mitochondrial Unfolded Protein Responses in White Adipose Tissue: Lipoatrophy, Whole-Body Metabolism and Lifespan
Authors: Kobayashi Masaki , Nezu Yuichiro , Tagawa Ryoma , Higami Yoshikazu
Published: March 2021

The mitochondrial unfolded protein response (UPRmt) is a stress response mediated by the expression of genes such as chaperones, proteases, and mitokines to maintain mitochondrial proteostasis. Certain genetically modified mice, which defect mitochondrial proteins specifically in adipocytes, developed atrophy of the white adipose tissue, resisted diet-induced obesity, and had altered whole-body metabolism. UPRmt, which has beneficial functions for living organisms, is termed “mitohormesis”, but its specific characteristics and detailed regulatory mechanism have not been elucidated to date. In this review, we discuss the function of UPRmt in adipose atrophy (lipoatrophy), whole-body metabolism, and lifespan based on the concept of mitohormesis.
Combining Everolimus and Ku0063794 Promotes Apoptosis of Hepatocellular Carcinoma Cells via Reduced Autophagy Resulting from Diminished Expression of miR-4790-3p
Authors: Choi Ho , Park Jung , Kim Ok-Hee , Kim Kee-Hwan , Hong Ha , Seo Haeyeon , Kim Say-June
Published: March 2021

It is challenging to overcome the low response rate of everolimus in the treatment of patients with hepatocellular carcinoma (HCC). To overcome this challenge, we combined everolimus with Ku0063794, the inhibitor of mTORC1 and mTORC2, to achieve higher anticancer effects. However, the precise mechanism for the synergistic effects is not clearly understood yet. To achieve this aim, the miRNAs were selected that showed the most significant variation in expression according to the mono- and combination therapy of everolimus and Ku0063794. Subsequently, the roles of specific miRNAs were determined in the processes of the treatment modalities. Compared to individual monotherapies, the combination therapy significantly reduced viability, increased apoptosis, and reduced autophagy in HepG2 cells. The combination therapy led to significantly lower expression of miR-4790-3p and higher expression of zinc finger protein225 (ZNF225)—the predicted target of miR-4790-3p. The functional study of miR-4790-3p and ZNF225 revealed that regarding autophagy, miR-4790-3p promoted it, while ZNF225 inhibited it. In addition, regarding apoptosis, miR-4790-3p inhibited it, while ZNF225 promoted it. It was also found that HCC tissues were characterized by higher expression of miR-4790-3p and lower expression of ZNF225; HCC tissues were also characterized by higher autophagic flux. We, thus, conclude that the potentiated anticancer effect of the everolimus and Ku0063794 combination therapy is strongly associated with reduced autophagy resulting from diminished expression of miR-4790-3p, as well as higher expression of ZNF225.
Post-Translational Modifications of Retroviral HIV-1 Gag Precursors: An Overview of Their Biological Role
Authors: Bussienne Charlotte , Marquet Roland , Paillart Jean-Christophe , Bernacchi Serena
Published: March 2021

Protein post-translational modifications (PTMs) play key roles in eukaryotes since they finely regulate numerous mechanisms used to diversify the protein functions and to modulate their signaling networks. Besides, these chemical modifications also take part in the viral hijacking of the host, and also contribute to the cellular response to viral infections. All domains of the human immunodeficiency virus type 1 (HIV-1) Gag precursor of 55-kDa (Pr55Gag), which is the central actor for viral RNA specific recruitment and genome packaging, are post-translationally modified. In this review, we summarize the current knowledge about HIV-1 Pr55Gag PTMs such as myristoylation, phosphorylation, ubiquitination, sumoylation, methylation, and ISGylation in order to figure out how these modifications affect the precursor functions and viral replication. Indeed, in HIV-1, PTMs regulate the precursor trafficking between cell compartments and its anchoring at the plasma membrane, where viral assembly occurs. Interestingly, PTMs also allow Pr55Gag to hijack the cell machinery to achieve viral budding as they drive recognition between viral proteins or cellular components such as the ESCRT machinery. Finally, we will describe and compare PTMs of several other retroviral Gag proteins to give a global overview of their role in the retroviral life cycle.
Cytokine Signature of Dengue Patients at Different Severity of the Disease
Authors: Puc Irwin , Ho Tzu-Chuan , Yen Ko-Lun , Vats Amrita , Tsai Jih-Jin , Chen Po-Lin , Chien Yu-Wen , Lo Yu-Chih , Perng Guey Chuen
Published: March 2021

Clinical presentations of dengue fever (DF) are diverse and non-specific, causing unpredictable progression and outcomes. Its progression and severity have been associated with cytokine levels alteration. In this study, dengue patients were classified into groups following the 2009 WHO dengue classification scheme to investigate the cytokine signature at different severity of the disease: dengue without warning sign symptoms (A); dengue with warning signs (B); severe dengue (C); other fever (OF) and healthy (Healthy). We analyzed 23 different cytokines simultaneously, namely IL-1b, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-33, CD14, CD54, CD62E, CD62L, CD62p, CD106, CD121b, CD154, CD178, GM-CSF, IFN-g, MIF, ST2 and TNF from patients admitted to National Cheng Kung University Hospital during the 2015 Taiwan dengue outbreak. Cytokines TNF, CD54, CD62E, CD62L, CD62P, GM-CSF, IL-1b, IL-2, IL-6, IL-8, IL-10, IL-12p70, IL-17A, INF-g and MIF were elevated while CD106, CD154, IL-4 and L-33 were decreased when compared to the control. IL-10 demonstrated to be a potential diagnostic marker for DF (H and A group; AUC = 0.944, H and OF group; AUC = 0.969). CD121b demonstrated to be predictive of the SD (A and B group; AUC = 0.744, B and C group; AUC = 0.775). Our results demonstrate the cytokine profile changes during the progression of dengue and highlight possible biomarkers for optimizing effective intervention strategies.
Comparisons of Extracellular Vesicles from Human Epidural Fat-Derived Mesenchymal Stem Cells and Fibroblast Cells
Authors: Sung Soo-Eun , Kang Kyung-Ku , Choi Joo-Hee , Lee Si-Joon , Kim KilSoo , Lim Ju-Hyeon , Yang Seung Yun , Kim Seul-Ki , Seo Min-Soo , Lee Gun Woo
Published: March 2021

Extracellular vesicles (EVs) are generated and secreted by cells into the circulatory system. Stem cell-derived EVs have a therapeutic effect similar to that of stem cells and are considered an alternative method for cell therapy. Accordingly, research on the characteristics of EVs is emerging. EVs were isolated from human epidural fat-derived mesenchymal stem cells (MSCs) and human fibroblast culture media by ultracentrifugation. The characterization of EVs involved the typical evaluation of cluster of differentiation (CD antigens) marker expression by fluorescence-activated cell sorting, size analysis with dynamic laser scattering, and morphology analysis with transmission electron microscopy. Lastly, the secreted levels of cytokines and chemokines in EVs were determined by a cytokine assay. The isolated EVs had a typical size of approximately 30–200 nm, and the surface proteins CD9 and CD81 were expressed on human epidural fat MSCs and human fibroblast cells. The secreted levels of cytokines and chemokines were compared between human epidural fat MSC-derived EVs and human fibroblast-derived EVs. Human epidural fat MSC-derived EVs showed anti-inflammatory effects and promoted macrophage polarization. In this study, we demonstrated for the first time that human epidural fat MSC-derived EVs exhibit inflammatory suppressive potency relative to human fibroblast-derived EVs, which may be useful for the treatment of inflammation-related diseases.
Muscle Damage in Systemic Sclerosis and CXCL10: The Potential Therapeutic Role of PDE5 Inhibition
Authors: Corinaldesi Clarissa , Ross Rebecca L. , Abignano Giuseppina , Antinozzi Cristina , Marampon Francesco , di Luigi Luigi , Buch Maya H. , Riccieri Valeria , Lenzi Andrea , Crescioli Clara , Del Galdo Francesco
Published: March 2021

Skeletal muscle damage is a common clinical manifestation of systemic sclerosis (SSc). C-X-C chemokine ligand 10 (CXCL10) is involved in myopathy and cardiomyopathy development and is associated with a more severe SSc prognosis. Interestingly, the phosphodiesterase type 5 inhibitor (PDE5i) sildenafil reduces CXCL10 sera levels of patients with diabetic cardiomyopathy and in cardiomyocytes. Here, we analyzed the levels of CXCL10 in the sera of 116 SSc vs. 35 healthy subjects and explored differences in 17 SSc patients on stable treatment with sildenafil. CXCL10 sera levels were three-fold higher in SSc vs. healthy controls, independent of subset and antibody positivity. Sildenafil treatment was associated with lower CXCL10 sera levels. Serum CXCL10 strongly correlated with the clinical severity of muscle involvement and with creatine kinase (CK) serum concentration, suggesting a potential involvement in muscle damage in SSc. In vitro, sildenafil dose-dependently reduced CXCL10 release by activated myocytes and impaired cytokine-induced Signal transducer and activator of transcription 1 (STAT1), Nuclear factor-κB (NFκB) and c-Jun N-terminal kinase (JNK) phosphorylation. This was also seen in cardiomyocytes. Sildenafil-induced CXCL10 inhibition at the systemic and human muscle cell level supports the hypothesis that PDE5i could be a potential therapeutic therapy to prevent and treat muscle damage in SSc.
Essential Role of Non-Coding RNAs in Enterovirus Infection: From Basic Mechanisms to Clinical Prospects
Authors: Zhu Peiyu , Chen Shuaiyin , Zhang Weiguo , Duan Guangcai , Jin Yuefei
Published: March 2021

Enteroviruses (EVs) are common RNA viruses that can cause various types of human diseases and conditions such as hand, foot, and mouth disease (HFMD), myocarditis, meningitis, sepsis, and respiratory disorders. Although EV infections in most patients are generally mild and self-limiting, a small number of young children can develop serious complications such as encephalitis, acute flaccid paralysis, myocarditis, and cardiorespiratory failure, resulting in fatalities. Established evidence has suggested that certain non-coding RNAs (ncRNAs) such as microRNAs (miRNAs), long ncRNAs (lncRNAs), and circular RNAs (circRNAs) are involved in the occurrence and progression of many human diseases. Recently, the involvement of ncRNAs in the course of EV infection has been reported. Herein, the authors focus on recent advances in the understanding of ncRNAs in EV infection from basic viral pathogenesis to clinical prospects, providing a reference basis and new ideas for disease prevention and research directions.
A Novel Approach for Classification and Forecasting of Time Series in Particle Accelerators
Authors: Li Sichen , Zacharias Mélissa , Snuverink Jochem , Coello de Portugal Jaime , Perez-Cruz Fernando , Reggiani Davide , Adelmann Andreas
Published: March 2021

The beam interruptions (interlocks) of particle accelerators, despite being necessary safety measures, lead to abrupt operational changes and a substantial loss of beam time. A novel time series classification approach is applied to decrease beam time loss in the High-Intensity Proton Accelerator complex by forecasting interlock events. The forecasting is performed through binary classification of windows of multivariate time series. The time series are transformed into Recurrence Plots which are then classified by a Convolutional Neural Network, which not only captures the inner structure of the time series but also uses the advances of image classification techniques. Our best-performing interlock-to-stable classifier reaches an Area under the ROC Curve value of 0.71±0.01 compared to 0.65±0.01 of a Random Forest model, and it can potentially reduce the beam time loss by 0.5±0.2 s per interlock.
Counterintelligence Technologies: An Exploratory Case Study of Preliminary Credibility Assessment Screening System in the Afghan National Defense and Security Forces
Authors: Reis João , Amorim Marlene , Melão Nuno , Cohen Yuval , Costa Joana
Published: March 2021

The preliminary credibility assessment screening system (PCASS) is a US-based program, which is currently being implemented by intelligence units of the North Atlantic Treaty Organization (NATO) to make the initial screening of individuals suspected of infiltrating the Afghan National Defense and Security Forces (ANDSF). Sensors have been instrumental in the PCASS, leading to organizational change. The aim of this research is to describe how the ANDSF adapted to the implementation of PCASS, as well as implemented changes since the beginning of the program. To do so, we have conducted a qualitative, exploratory, and descriptive case study that allows one to understand, through the use of a series of data collection sources, a real-life phenomenon of which little is known. The results suggest that the sensors used in PCASS empower security forces with reliable technologies to identify and neutralize internal threats. It then becomes evident that the technological leadership that PCASS provides allows the developing of a relatively stable and consistent organizational change, fulfilling the objectives of the NATO and the ANDSF.
Multiscale Characterization of E-Glass/Epoxy Composite Exposed to Extreme Environmental Conditions
Authors: Youssef George , Newacheck Scott , Huynh Nha Uyen , Gamez Carlos
Published: March 2021

Fiber-reinforced polymer matrix composites continue to attract scientific and industrial interest since they offer superior strength-, stiffness-, and toughness-to-weight ratios. The research herein characterizes two sets of E-Glass/Epoxy composite skins: stressed and unstressed. The stressed samples were previously installed in an underground power distribution vault and were exposed to fire while the unstressed composite skins were newly fabricated and never-deployed samples. The mechanical, morphological, and elemental composition of the samples were methodically studied using a dynamic mechanical analyzer, a scanning electron microscope (SEM), and an x-ray diffractometer, respectively. Sandwich composite panels consisting of E-glass/Epoxy skin and balsa wood core were originally received, and the balsa wood was removed before any further investigations. Skin-only specimens with dimensions of ~12.5 mm wide, ~70 mm long, and ~6 mm thick were tested in a Dynamic Mechanical Analyzer in a dual-cantilever beam configuration at 5 Hz and 10 Hz from room temperature to 210 °C. Micrographic analysis using the SEM indicated a slight change in morphology due to the fire event but confirmed the effectiveness of the fire-retardant agents in quickly suppressing the fire. Accompanying Fourier transform infrared and energy dispersive X-ray spectroscopy studies corroborated the mechanical and morphological results. Finally, X-ray diffraction showed that the fire event consumed the surface level fire-retardant and the structural attributes of the E-Glass/Epoxy remained mainly intact. The results suggest the panels can continue field deployment, even after short fire incident.
Restoration of Missing Patterns on Satellite Infrared Sea Surface Temperature Images Due to Cloud Coverage Using Deep Generative Inpainting Network
Authors: Kang Song-Hee , Choi Youngjin , Choi Jae Young
Published: March 2021

In this paper, we propose a novel deep generative inpainting network (GIN) trained under the framework of generative adversarial learning, which is optimized for the restoration of cloud-disturbed satellite sea surface temperature (SST) imagery. The proposed GIN architecture can achieve accurate and fast restoration results. The proposed GIN consists of rough and fine reconstruction stages to promote the details and textures of missing (clouded) regions in SST images. We also propose a nov el preprocessing strategy that replaces the land areas with the average value of daily oceanic surface temperatures for improving restoration accuracy. To learn the proposed GIN, we developed a novel approach that combines multiple loss functions well suited for improving the restoration quality over missing SST information. Our results show that the difference in temperature between restored and actual satellite image data was no larger than 0.7 °C in monthly average values, which suggests excellent resilience against the missing sea surface temperature data. The proposed GIN has a faster restoration time and is feasible for real-time ocean-related applications. Furthermore, the computational cost of restoring SST images is much lower than the popular interpolation methods.
In Vitro Combination Effect of Topical and Oral Anti-Onychomycosis Drugs on Trichophyton rubrum and Trichophyton interdigitale
Authors: Sugiura Keita , Masumoto Akane , Tachibana Haruki , Tatsumi Yoshiyuki
Published: March 2021

To evaluate the combination effects of anti-onychomycosis drugs, the minimum inhibitory concentrations of topical (efinaconazole, luliconazole, and tavaborole) and oral (itraconazole and terbinafine) drugs for Trichophyton rubrum and Trichophyton interdigitale (8 each, with a total of 16 strains) were determined using the microdilution checkerboard technique based on the Clinical and Laboratory Standard Institute guidelines. No antagonism was observed between the topical and oral drugs against all the tested strains. Efinaconazole with terbinafine exerted a synergistic effect on 43.8% of the strains tested (7/16 strains) and efinaconazole with itraconazole on 12.5% (2/16 strains). Conversely, luliconazole showed no synergistic effect with terbinafine but was synergistically effective with itraconazole against 31.3% of the strains (5/16 strains). Tavaborole showed no synergistic effect with terbinafine and was synergistically effective with itraconazole against 18.8% of the strains (3/16 strains). The results suggest that a combination of topical and oral drugs could be a potential clinical option for onychomycosis treatment, and overall, the efinaconazole and oral drug combination would be the most advantageous among the tested combinations.
The Influence Mechanism of Ettringite Crystals and Microstructure Characteristics on the Strength of Calcium-Based Stabilized Soil
Authors: Han Youmin , Xia Junwu , Chang Hongfei , Xu Jun
Published: March 2021

To reveal the influence mechanism of ettringite (AFt) crystals and microstructure characteristics on the strength of calcium-based stabilized soil, the strengths and microscopic properties of seven groups of stabilized soil samples were studied systematically through unconfined compressive strength, scanning electron microscope (SEM), X-ray diffraction (XRD), thermogravimetry (TG), and Fourier transform infrared spectroscopy (FTIR) testing methods. The results indicate that the strength of the cement-stabilized soil is relatively high because abundant calcium silicate hydrate (CSH) gels coat the outer surface of soil particles to cement together. For the cement–gypsum-stabilized soil, superabundant thick and long AFt crystals make the pores in soil particles larger, and the sample becomes looser, resulting in lower strength than that of the cement-stabilized soil. However, the strength of the cement–gypsum–lime-stabilized soil is slightly stronger than that of the cement-stabilized soil, for the reason that the appropriate amount of fine AFt crystals fill the macropores between soil particles to form a network space structure and sufficient CSH gels cement the soil particles and the AFt crystals network space structure tightly together. It could be suggested that the components of calcium-based stabilizer should consider the optimal production balance between CSH gels and fine AFt crystals.
Energy Absorption and Mechanical Performance of Functionally Graded Soft–Hard Lattice Structures
Authors: Rahman Hafizur , Yarali Ebrahim , Zolfagharian Ali , Serjouei Ahmad , Bodaghi Mahdi
Published: March 2021

Today, the rational combination of materials and design has enabled the development of bio-inspired lattice structures with unprecedented properties to mimic biological features. The present study aims to investigate the mechanical performance and energy absorption capacity of such sophisticated hybrid soft–hard structures with gradient lattices. The structures are designed based on the diversity of materials and graded size of the unit cells. By changing the unit cell size and arrangement, five different graded lattice structures with various relative densities made of soft and hard materials are numerically investigated. The simulations are implemented using ANSYS finite element modeling (FEM) (2020 R1, 2020, ANSYS Inc., Canonsburg, PA, USA) considering elastic-plastic and the hardening behavior of the materials and geometrical non-linearity. The numerical results are validated against experimental data on three-dimensional (3D)-printed lattices revealing the high accuracy of the FEM. Then, by combination of the dissimilar soft and hard polymeric materials in a homogenous hexagonal lattice structure, two dual-material mechanical lattice statures are designed, and their mechanical performance and energy absorption are studied. The results reveal that not only gradual changes in the unit cell size provide more energy absorption and improve mechanical performance, but also the rational combination of soft and hard materials make the lattice structure with the maximum energy absorption and stiffness, in comparison to those structures with a single material, interesting for multi-functional applications.
Dual-Motor Planetary Transmission to Improve Efficiency in Electric Vehicles
Authors: Mantriota Giacomo , Reina Giulio
Published: March 2021

Electric cars are typically subject to highly variable operational conditions, especially when they drive in urban environments. Consequently, the efficiency of the electric motors may degrade significantly, possibly leading to lower autonomy and higher running costs. Latest advances in power electronics and motion control have paved the way to the development of novel architectures of full electric power transmissions. In this paper, a dual-motor solution is proposed where two smaller motors are coupled via a planetary gear, in contrast to the standard configuration that uses one larger motor directly connected to the drive wheels with a fixed ratio reducer. The dual-motor architecture guarantees that both motors operate in the vicinity of their optimal working range, resulting in a higher overall energy efficiency. The technical requirements and the control strategy of the dual-motor system are selected through a parametric optimization process. Results included were obtained from extensive simulations performed over different standard driving cycles, showing that the dual-motor power transmission generally outperforms the single-motor counterpart with an average efficiency improvement of about 9% that is reached in both the power delivery and regeneration stage.
Nonvolatile Voltage Controlled Molecular Spin-State Switching for Memory Applications
Authors: Ekanayaka Thilini K. , Hao Guanhua , Mosey Aaron , Dale Ashley S. , Jiang Xuanyuan , Yost Andrew J. , Sapkota Keshab R. , Wang George T. , Zhang Jian , N’Diaye Alpha T. , Marshall Andrew , Cheng Ruihua , Naeemi Azad , Xu Xiaoshan , Dowben Peter A.
Published: March 2021

Nonvolatile, molecular multiferroic devices have now been demonstrated, but it is worth giving some consideration to the issue of whether such devices could be a competitive alternative for solid-state nonvolatile memory. For the Fe (II) spin crossover complex [Fe{H2B(pz)2}2(bipy)], where pz = tris(pyrazol-1-yl)-borohydride and bipy = 2,2′-bipyridine, voltage-controlled isothermal changes in the electronic structure and spin state have been demonstrated and are accompanied by changes in conductance. Higher conductance is seen with [Fe{H2B(pz)2}2(bipy)] in the high spin state, while lower conductance occurs for the low spin state. Plausibly, there is the potential here for low-cost molecular solid-state memory because the essential molecular thin films are easily fabricated. However, successful device fabrication does not mean a device that has a practical value. Here, we discuss the progress and challenges yet facing the fabrication of molecular multiferroic devices, which could be considered competitive to silicon.
Cavalier Use of Inferential Statistics Is a Major Source of False and Irreproducible Scientific Findings
Authors: Hanin Leonid
Published: March 2021

I uncover previously underappreciated systematic sources of false and irreproducible results in natural, biomedical and social sciences that are rooted in statistical methodology. They include the inevitably occurring deviations from basic assumptions behind statistical analyses and the use of various approximations. I show through a number of examples that (a) arbitrarily small deviations from distributional homogeneity can lead to arbitrarily large deviations in the outcomes of statistical analyses; (b) samples of random size may violate the Law of Large Numbers and thus are generally unsuitable for conventional statistical inference; (c) the same is true, in particular, when random sample size and observations are stochastically dependent; and (d) the use of the Gaussian approximation based on the Central Limit Theorem has dramatic implications for p-values and statistical significance essentially making pursuit of small significance levels and p-values for a fixed sample size meaningless. The latter is proven rigorously in the case of one-sided Z test. This article could serve as a cautionary guidance to scientists and practitioners employing statistical methods in their work.
Metabolomic Profiling Reveals Sex Specific Associations with Chronic Obstructive Pulmonary Disease and Emphysema
Authors: Gillenwater Lucas A. , Kechris Katerina J. , Pratte Katherine A. , Reisdorph Nichole , Petrache Irina , Labaki Wassim W. , O’Neal Wanda , Krishnan Jerry A. , Ortega Victor E. , DeMeo Dawn L. , Bowler Russell P.
Published: March 2021

Susceptibility and progression of lung disease, as well as response to treatment, often differ by sex, yet the metabolic mechanisms driving these sex-specific differences are still poorly understood. Women with chronic obstructive pulmonary disease (COPD) have less emphysema and more small airway disease on average than men, though these differences become less pronounced with more severe airflow limitation. While small studies of targeted metabolites have identified compounds differing by sex and COPD status, the sex-specific effect of COPD on systemic metabolism has yet to be interrogated. Significant sex differences were observed in 9 of the 11 modules identified in COPDGene. Sex-specific associations by COPD status and emphysema were observed in 3 modules for each phenotype. Sex stratified individual metabolite associations with COPD demonstrated male-specific associations in sphingomyelins and female-specific associations in acyl carnitines and phosphatidylethanolamines. There was high preservation of module assignments in SPIROMICS (SubPopulations and InteRmediate Outcome Measures In COPD Study) and similar female-specific shift in acyl carnitines. Several COPD associated metabolites differed by sex. Acyl carnitines and sphingomyelins demonstrate sex-specific abundances and may represent important metabolic signatures of sex differences in COPD. Accurately characterizing the sex-specific molecular differences in COPD is vital for personalized diagnostics and therapeutics.