bluuebunny/crossref_metadata_2025 huggingface dataset:
Dual Action of Sulfated Hyaluronan on Angiogenic Processes in Relation to Vascular Endothelial Growth Factor-A
Authors: Koehler Linda , Ruiz-Gómez Gloria , Balamurugan Kanagasabai , Rother Sandra , Freyse Joanna , Möller Stephanie , Schnabelrauch Matthias , Köhling Sebastian , Djordjevic Snezana , Scharnweber Dieter , Rademann Jörg , Pisabarro M. Teresa , Hintze Vera
Published: December 2019

Pathological healing characterized by abnormal angiogenesis presents a serious burden to patients’ quality of life requiring innovative treatment strategies. Glycosaminoglycans (GAG) are important regulators of angiogenic processes. This experimental and computational study revealed how sulfated GAG derivatives (sGAG) influence the interplay of vascular endothelial growth factor (VEGF)165 and its heparin-binding domain (HBD) with the signaling receptor VEGFR-2 up to atomic detail. There was profound evidence for a HBD-GAG-HBD stacking configuration. Here, the sGAG act as a “molecular glue” leading to recognition modes in which sGAG interact with two VEGF165-HBDs. A 3D angiogenesis model demonstrated the dual regulatory role of high-sulfated derivatives on the biological activity of endothelial cells. While GAG alone promote sprouting, they downregulate VEGF165-mediated signaling and, thereby, elicit VEGF165-independent and -dependent effects. These findings provide novel insights into the modulatory potential of sGAG derivatives on angiogenic processes and point towards their prospective application in treating abnormal angiogenesis.
Role of zinc substitution in magnetic hyperthermia properties of magnetite nanoparticles: interplay between intrinsic properties and dipolar interactions
Authors: Hadadian Yaser , Ramos Ana Paula , Pavan Theo Z.
Published: December 2019

Optimizing the intrinsic properties of magnetic nanoparticles for magnetic hyperthermia is of considerable concern. In addition, the heating efficiency of the nanoparticles can be substantially influenced by dipolar interactions. Since adequate control of the intrinsic properties of magnetic nanoparticles is not straightforward, experimentally studying the complex interplay between these properties and dipolar interactions affecting the specific loss power can be challenging. Substituting zinc in magnetite structure is considered as an elegant approach to tune its properties. Here, we present experimental and numerical simulation results of magnetic hyperthermia studies using a series of zinc-substituted magnetite nanoparticles (ZnxFe1-xFe2O4, x = 0.0, 0.1, 0.2, 0.3 and 0.4). All experiments were conducted in linear regime and the results were inferred based on the numerical simulations conducted in the framework of the linear response theory. The results showed that depending on the nanoparticles intrinsic properties, interparticle interactions can have different effects on the specific loss power. When dipolar interactions were strong enough to affect the heating efficiency, the parameter σ = KeffV/kBT (Keff is the effective anisotropy and V the volume of the particles) determined the type of the effect. Finally, the sample x = 0.1 showed a superior performance with a relatively high intrinsic loss power 5.4 nHm2kg−1.
Stress-controlled decomposition routes in cubic AlCrN films assessed by in-situ high-temperature high-energy grazing incidence transmission X-ray diffraction
Authors: Meindlhumer M. , Klima S. , Jäger N. , Stark A. , Hruby H. , Mitterer C. , Keckes J. , Daniel R.
Published: December 2019

The dependence of decomposition routes on intrinsic microstructure and stress in nanocrystalline transition metal nitrides is not yet fully understood. In this contribution, three Al0.7Cr0.3N thin films with residual stress magnitudes of −3510, −4660 and −5930 MPa in the as-deposited state were in-situ characterized in the range of 25–1100 °C using in-situ synchrotron high-temperature high-energy grazing-incidence-transmission X-ray diffraction and temperature evolutions of phases, coefficients of thermal expansion, structural defects, texture as well as residual, thermal and intrinsic stresses were evaluated. The multi-parameter experimental data indicate a complex intrinsic stress and phase changes governed by a microstructure recovery and phase transformations taking place above the deposition temperature. Though the decomposition temperatures of metastable cubic Al0.7Cr0.3N phase in the range of 698–914 °C are inversely proportional to the magnitudes of deposition temperatures, the decomposition process itself starts at the same stress level of ~−4300 MPa in all three films. This phenomenon indicates that the particular compressive stress level functions as an energy threshold at which the diffusion driven formation of hexagonal Al(Cr)N phase is initiated, provided sufficient temperature is applied. In summary, the unique synchrotron experimental setup indicated that residual stresses play a decisive role in the decomposition routes of nanocrystalline transition metal nitrides.
Markov versus quantum dynamic models of belief change during evidence monitoring
Authors: Busemeyer Jerome R. , Kvam Peter D. , Pleskac Timothy J.
Published: December 2019

Two different dynamic models for belief change during evidence monitoring were evaluated: Markov and quantum. They were empirically tested with an experiment in which participants monitored evidence for an initial period of time, made a probability rating, then monitored more evidence, before making a second rating. The models were qualitatively tested by manipulating the time intervals in a manner that provided a test for interference effects of the first rating on the second. The Markov model predicted no interference, whereas the quantum model predicted interference. More importantly, a quantitative comparison of the two models was also carried out using a generalization criterion method: the parameters were fit to data from one set of time intervals, and then these same parameters were used to predict data from another set of time intervals. The results indicated that some features of both Markov and quantum models are needed to accurately account for the results.
Mid-Cretaceous amber inclusions reveal morphogenesis of extinct rachis-dominated feathers
Authors: Carroll Nathan R. , Chiappe Luis M. , Bottjer David J.
Published: December 2019

We describe three-dimensionally preserved feathers in mid-Cretaceous Burmese amber that share macro-morphological similarities (e.g., proportionally wide rachis with a “medial stripe”) with lithic, two-dimensionally preserved rachis-dominated feathers, first recognized in the Jehol Biota. These feathers in amber reveal a unique ventrally concave and dorsoventrally thin rachis, and a dorsal groove (sometimes pigmented) that we identify as the “medial stripe” visible in many rachis-dominated rectrices of Mesozoic birds. The distally pennaceous portion of these feathers shows differentiated proximal and distal barbules, the latter with hooklets forming interlocking barbs. Micro-CT scans and transverse sections demonstrate the absence of histodifferentiated cortex and medullary pith of the rachis and barb rami. The highly differentiated barbules combined with the lack of obvious histodifferentiation of the barb rami or rachis suggests that these feathers could have been formed without the full suite and developmental interplay of intermediate filament alpha keratins and corneous beta-proteins that is employed in the cornification process of modern feathers. This study thus highlights how the development of these feathers might have differed from that of their modern counterparts, namely in the morphogenesis of the ventral components of the rachis and barb rami. We suggest that the concave ventral surface of the rachis of these Cretaceous feathers is not homologous with the ventral groove of modern rachises. Our study of these Burmese feathers also confirms previous claims, based on two-dimensional fossils, that they correspond to an extinct morphotype and it cautions about the common practice of extrapolating developmental aspects (and mechanical attributes) of modern feathers to those of stem birds (and their dinosaurian outgroups) because the latter need not to have developed through identical pathways.
BioSphincters to treat Fecal Incontinence in Nonhuman Primates
Authors: Dadhich Prabhash , Bohl Jaime L. , Tamburrini Riccardo , Zakhem Elie , Scott Christie , Kock Nancy , Mitchell Erin , Gilliam John , Bitar Khalil N.
Published: December 2019

Loss of anorectal resting pressure due to internal anal sphincter (IAS) dysfunctionality causes uncontrolled fecal soiling and leads to passive fecal incontinence (FI). The study is focused on immediate and long-term safety and potential efficacy of bioengineered IAS BioSphincters to treat passive FI in a clinically relevant large animal model of passive FI. Passive FI was successfully developed in Non-Human Primates (NHPs) model. The implantation of autologous intrinsically innervated functional constructs resolved the fecal soiling, restored the resting pressure and Recto Anal Inhibitory Reflex (RAIR) within 1-month. These results were sustained with time, and efficacy was preserved up to 12-months. The histological studies validated manometric results with the regeneration of a well-organized neuro-muscular population in IAS. The control groups (non-treated and sham) remained affected by poor anal hygiene, lower resting pressure, and reduced RAIR throughout the study. The pathological assessment of implants, blood, and the vital organs confirmed biocompatibility without any adverse effect after implantation. This regenerative approach of implanting intrinsically innervated IAS BioSphincters has the potential to offer a better quality of life to the patients suffering from FI.
Metabolic shift induced by synthetic co-cultivation promotes high yield of chain elongated acids from syngas
Authors: Diender Martijn , Parera Olm Ivette , Gelderloos Marten , Koehorst Jasper J. , Schaap Peter J. , Stams Alfons J. M. , Sousa Diana Z.
Published: December 2019

Bio-catalytic processes for sustainable production of chemicals and fuels receive increased attention within the concept of circular economy. Strategies to improve these production processes include genetic engineering of bio-catalysts or process technological optimization. Alternatively, synthetic microbial co-cultures can be used to enhance production of chemicals of interest. It remains often unclear however how microbe to microbe interactions affect the overall production process and how this can be further exploited for application. In the present study we explored the microbial interaction in a synthetic co-culture of Clostridium autoethanogenum and Clostridium kluyveri, producing chain elongated products from carbon monoxide. Monocultures of C. autoethanogenum converted CO to acetate and traces of ethanol, while during co-cultivation with C. kluyveri, it shifted its metabolism significantly towards solventogenesis. In C. autoethanogenum, expression of the genes involved in the central carbon- and energy-metabolism remained unchanged during co-cultivation compared to monoculture condition. Therefore the shift in the metabolic flux of C. autoethanogenum appears to be regulated by thermodynamics, and results from the continuous removal of ethanol by C. kluyveri. This trait could be further exploited, driving the metabolism of C. autoethanogenum to solely ethanol formation during co-cultivation, resulting in a high yield of chain elongated products from CO-derived electrons. This research highlights the important role of thermodynamic interactions in (synthetic) mixed microbial communities and shows that this can be exploited to promote desired conversions.
High throughput barcoding method for genome-scale phasing
Authors: Redin David , Frick Tobias , Aghelpasand Hooman , Käller Max , Borgström Erik , Olsen Remi-Andre , Ahmadian Afshin
Published: December 2019

The future of human genomics is one that seeks to resolve the entirety of genetic variation through sequencing. The prospect of utilizing genomics for medical purposes require cost-efficient and accurate base calling, long-range haplotyping capability, and reliable calling of structural variants. Short-read sequencing has lead the development towards such a future but has struggled to meet the latter two of these needs. To address this limitation, we developed a technology that preserves the molecular origin of short sequencing reads, with an insignificant increase to sequencing costs. We demonstrate a novel library preparation method for high throughput barcoding of short reads where millions of random barcodes can be used to reconstruct megabase-scale phase blocks.
Life-span extension by pigmented rice bran in the model yeast Saccharomyces cerevisiae
Authors: Sunthonkun Pitchapat , Palajai Rinsai , Somboon Pichayada , Suan Chua Lee , Ungsurangsri Malyn , Soontorngun Nitnipa
Published: December 2019

Benefits of whole grains as dietary supplements and active ingredients in health products have been promoted. Despite being neglected as an agricultural byproduct of polished rice, pigmented rice bran has emerged as a promising source of natural anti-aging compounds. Indeed, the extract of red rice bran Hom Dang cultivar contained rich phenolic acids and flavonoids. It displayed high antioxidant activities in vitro and in vivo assays. Using yeast model, extract and bioactive compounds, quercetin and protocatechuic acid found in the rice bran pericarp, effectively reduced levels of intracellular reactive oxygen species (ROS), restored plasma membrane damages and prolonged life-span of pre-treated wild-yeast cells. Importantly, these molecules modulated life span-extension through a mechanism of ROS reduction that resembles to that operated under the highly conserved Tor1- and Sir2-dependent signaling pathways, with the human homologs TORC1 and SIRT1, respectively. The key longevity factors Sch9 and Rim15 kinases, Msn2/4 regulators and a novel transcription factor Asg1, the antioxidant enzymes superoxide dismutases and glutathione peroxidases played important role in mediating longevity. Yeast clearly provides an instrumental platform for rapid screening of compounds with anti-aging efficacies and advances knowledge in the molecular study of ageing.
An integrated screening system for the selection of exemplary substrates for natural and engineered cytochrome P450s
Authors: Kanoh Naoki , Kawamata-Asano Ayano , Suzuki Kana , Takahashi Yusuke , Miyazawa Takeshi , Nakamura Takemichi , Moriya Takashi , Hirano Hiroyuki , Osada Hiroyuki , Iwabuchi Yoshiharu , Takahashi Shunji
Published: December 2019

Information about substrate and product selectivity is critical for understanding the function of cytochrome P450 monooxygenases. In addition, comprehensive understanding of changes in substrate selectivity of P450 upon amino acid mutation would enable the design and creation of engineered P450s with desired selectivities. Therefore, systematic methods for obtaining such information are required. Herein, we developed an integrated P450 substrate screening system for the selection of “exemplary” substrates for a P450 of interest. The established screening system accurately selected the known exemplary substrates and also identified previously unknown exemplary substrates for microbial-derived P450s from a library containing sp3-rich synthetic small molecules. Synthetically potent transformations were also found by analyzing the reactions and oxidation products. The screening system was applied to analyze the substrate selectivity of the P450 BM3 mutants F87A and F87A/A330W, which acquired an ability to hydroxylate non-natural substrate steroids regio- and stereoselectively by two amino acid mutations. The distinct transition of exemplary substrates due to each single amino acid mutation was revealed, demonstrating the utility of the established system.
Tropical forest soil carbon stocks do not increase despite 15 years of doubled litter inputs
Authors: Sayer Emma J. , Lopez-Sangil Luis , Crawford John A. , Bréchet Laëtitia M. , Birkett Ali J. , Baxendale Catherine , Castro Biancolini , Rodtassana Chadtip , Garnett Mark H. , Weiss Lena , Schmidt Michael W. I.
Published: December 2019

Soil organic carbon (SOC) dynamics represent a persisting uncertainty in our understanding of the global carbon cycle. SOC storage is strongly linked to plant inputs via the formation of soil organic matter, but soil geochemistry also plays a critical role. In tropical soils with rapid SOC turnover, the association of organic matter with soil minerals is particularly important for stabilising SOC but projected increases in tropical forest productivity could trigger feedbacks that stimulate the release of stored SOC. Here, we demonstrate limited additional SOC storage after 13–15 years of experimentally doubled aboveground litter inputs in a lowland tropical forest. We combined biological, physical, and chemical methods to characterise SOC along a gradient of bioavailability. After 13 years of monthly litter addition treatments, most of the additional SOC was readily bioavailable and we observed no increase in mineral-associated SOC. Importantly, SOC with weak association to soil minerals declined in response to long-term litter addition, suggesting that increased plant inputs could modify the formation of organo-mineral complexes in tropical soils. Hence, we demonstrate the limited capacity of tropical soils to sequester additional C inputs and provide insights into potential underlying mechanisms.
Landscape of heart proteome changes in a diet-induced obesity model
Authors: Vileigas Danielle F. , Harman Victoria M. , Freire Paula P. , Marciano Cecília L. C. , Sant’Ana Paula G. , de Souza Sérgio L. B. , Mota Gustavo A. F. , da Silva Vitor L. , Campos Dijon H. S. , Padovani Carlos R. , Okoshi Katashi , Beynon Robert J. , Santos Lucilene D. , Cicogna Antonio C.
Published: December 2019

Obesity is a pandemic associated with a high incidence of cardiovascular disease; however, the mechanisms are not fully elucidated. Proteomics may provide a more in-depth understanding of the pathophysiological mechanisms and contribute to the identification of potential therapeutic targets. Thus, our study evaluated myocardial protein expression in healthy and obese rats, employing two proteomic approaches. Male Wistar rats were established in two groups (n = 13/group): control diet and Western diet fed for 41 weeks. Obesity was determined by the adipose index, and cardiac function was evaluated in vivo by echocardiogram and in vitro by isolated papillary muscle analysis. Proteomics was based on two-dimensional gel electrophoresis (2-DE) along with mass spectrometry identification, and shotgun proteomics with label-free quantification. The Western diet was efficient in triggering obesity and impaired contractile function in vitro; however, no cardiac dysfunction was observed in vivo. The combination of two proteomic approaches was able to increase the cardiac proteomic map and to identify 82 differentially expressed proteins involved in different biological processes, mainly metabolism. Furthermore, the data also indicated a cardiac alteration in fatty acids transport, antioxidant defence, cytoskeleton, and proteasome complex, which have not previously been associated with obesity. Thus, we define a robust alteration in the myocardial proteome of diet-induced obese rats, even before functional impairment could be detected in vivo by echocardiogram.
Efficacy and safety of lavender essential oil (Silexan) capsules among patients suffering from anxiety disorders: A network meta-analysis
Authors: Yap Wuan Shuen , Dolzhenko Anton V. , Jalal Zahraa , Hadi Muhammad Abdul , Khan Tahir Mehmood
Published: December 2019

A systematic review and network-meta analysis (NMA) were performed to estimate significance of the anxiolytic effect of lavender essential oil taken as silexan capsules versus other comparators (i.e., placebo/paroxetine/lorazepam). The outcome of interest was Hamilton Anxiety Scale (HAMA). Weighted mean differences (WMD) were calculated to estimate the treatment effect at the confidence interval of 95%. League tables were generated using treatment effect, for all pairwise comparisons, where WMD < 0 favors the column-defining treatment. Five studies were identified with a total of 524 participants receiving treatment with silexan 80 mg and 121 participants taking silexan 160 mg. The NMA results indicated that consumption of silexan 160 mg resulted in higher decline of HAMA score [WMD −1.14 (−1.10, 3.39)] in comparison to silexan 80 mg, placebo [−2.20 (−4.64, 0.24)] and paroxetine [−1.24 (−5.34, 2.85)]. The effect of silexan 80 mg was observed to be same as that of paroxetine. Overall, silexan 160 mg was noticed to be a more efficient treatment giving significant decline in HAMA score across other comparators. However, no improvements in HAMA score was observed for the group receiving lorazepam 0.5 mg when compared to silexan 160 mg, silexan 80 mg, paroxetine 20 mg, and placebo.
Spatial-sweep steady-state pattern electroretinography can detect subtle differences in visual function among healthy adults
Authors: Minami Sakiko , Nagai Norihiro , Suzuki Misa , Kurihara Toshihide , Sonobe Hideki , Watanabe Kazuhiro , Shinoda Hajime , Takagi Hitoshi , Tsubota Kazuo , Ozawa Yoko
Published: December 2019

We aimed to establish a highly sensitive method for measuring visual function using spatial-sweep steady-state pattern electroretinography (swpPERG). Overall, 35 eyes of 35 healthy adults (18 men; mean age, 32.3 years) were examined using swpPERG, and the data were recorded using spatial-patterned and contrast-reversed stimuli of size 1 (thickest) to 6. Data were converted into frequency-domain using a Fourier transform and expressed by signal-to-noise ratio (SNR). The number of participants who showed SNR ≥ 1 was significantly lesser at stimulus sizes 5 and 6 compared with those at greater stimulus sizes. Among the data with SNR ≥ 1, SNRs were negatively correlated with age at stimulus size 5 (r = −0.500, P = 0.029), and positively correlated with macular volume evaluated by optical coherence tomography (OCT) within a 6-mm circle diameter from the fovea of the retinal nerve fibre layer at size 4 (r = 0.409, P = 0.025) and of the ganglion cell layer at size 5 (r = 0.567, P = 0.011). We found that SNRs of swpPERG, recorded using the EvokeDx system, were correlated with age and macular morphology in participants without diagnosed eye diseases. The system detected subtle differences in retinal function, which may help in early disease diagnosis and visual evaluation in neuroprotective interventions in the future.
Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions
Authors: Rodríguez Antonio , Guillemyn Brecht , Coucke Paul , Vaneechoutte Mario
Published: December 2019

Fungal infections, ranging from superficial to life-threatening infections, represent a major public health problem that affects 25% of the worldwide population. In this context, the study of host-pathogen interactions within the host is crucial to advance antifungal therapy. However, since fungal cells are usually outnumbered by host cells, the fungal transcriptome frequently remains uncovered. We compared three different methods to selectively lyse human cells from in vitro mixes, composed of Candida cells and peripheral blood mononuclear cells. In order to prevent transcriptional modification, the mixes were stored in RNAlater. We evaluated the enrichment of fungal cells through cell counting using microscopy and aimed to further enrich fungal nucleic acids by centrifugation and by reducing contaminant nucleic acids from the host. We verified the enrichment of fungal DNA and RNA through qPCR and RT-qPCR respectively and confirmed that the resulting RNA has high integrity scores, suitable for downstream applications. The enrichment method provided here, i.e., lysis with Buffer RLT followed by centrifugation, may contribute to increase the proportion of nucleic acids from fungi in clinical samples, thus promoting more comprehensive analysis of fungal transcriptional profiles. Although we focused on C. albicans, the enrichment may be applicable to other fungal pathogens.
Cytokines Levels and Salivary Microbiome Play A Potential Role in Oral Lichen Planus Diagnosis
Authors: Carvalho Maria Fernanda Marques Silva de , Cavalieri Denise , Do Nascimento Sabrina , Lourenço Talita Gomes Baeta , Ramos Danielle Viana Ribeiro , Pasqualin Denise da Cunha , Martins Leandro Aurélio Liporoni , Rocha Fernanda Agostini , Heller Débora , Marti Luciana
Published: December 2019

Oral lichen planus (OLP) is a chronic Th1-mediated inflammatory mucocutaneous disease of the skin and oral mucosa that can have various clinical presentations. Lesions are usually bilateral and often painful. While cutaneous Lichen Planus (LP) lesions are self-limiting, the oral lesions are chronic and rarely remissive. The diagnosis of oral lichen planus (OLP) is often challenging, and confirmation by histopathological criterion is generally advised. The aim of our study was to identify the cytokines present in OLP-suggestive lesions and in non-specific inflammatory lesions (NSIL) used as controls. Moreover, assess cytokines protein levels and oral microbiota composition in whole saliva samples. Histopathological analysis, immunohistochemistry and gene expression were used as techniques to analyze the oral mucosal tissue samples. ELISA was conducted to analyze salivary cytokine levels and 16S rRNA sequencing was used to determine the salivary microbiome. As a result we observed larger number of infiltrated lymphocytes (p = 0.025), as well, more T CD4 lymphocytes in the epithelial tissue (p = 0.006) in OLP samples compared to NSIL. In addition, the OLP samples displayed more apoptotic cells compared to NSIL (p = 0.047). Regarding the cytokine analysis, IFN-γ and IL-33 were more expressed in OLP lesions than in NSIL samples (p < 0.001; p = 0.026). Furthermore, our results demonstrated higher levels of IFN-γ protein expression in the saliva of OLP group compared to controls (p = 0.0156). We also observed noted differences in the oral microbiota composition between OLP and NSIL saliva samples. In conclusion, OLP lesions presented larger numbers of apoptotic and inflammatory cells, higher levels of IFN-γ and IL-33 compared to NSIL, and these lesions also differ regarding oral microbiota composition. These results are consistent with the Th-1-mediated chronic inflammation nature of oral lichen planus investigated lesions and displayed unique features that could be used as a diagnostic tool.
DPP8 is a novel therapeutic target for multiple myeloma
Authors: Sato Tsutomu , Tatekoshi Ayumi , Takada Kohichi , Iyama Satoshi , Kamihara Yusuke , Jawaid Paras , Rehman Mati Ur , Noguchi Kyo , Kondo Takashi , Kajikawa Sayaka , Arita Kotaro , Wada Akinori , Murakami Jun , Arai Miho , Yasuda Ichiro , Dang Nam H. , Hatano Ryo , Iwao Noriaki , Ohnuma Kei , Morimoto Chikao
Published: December 2019

Dipeptidyl peptidases (DPPs) are proteolytic enzymes that are ideal therapeutic targets in human diseases. Indeed, DPP4 inhibitors are widely used in clinical practice as anti-diabetic agents. In this paper, we show that DPP4 inhibitors also induced cell death in multiple human myeloma cells. Among five DPP4 inhibitors, only two of them, vildagliptin and saxagliptin, exhibited apparent cytotoxic effects on myeloma cell lines, without any difference in suppression of DPP4 activity. As these two DPP4 inhibitors are known to have off-target effects against DPP8/9, we employed the specific DPP8/9 inhibitor 1G244. 1G244 demonstrated anti-myeloma effects on several cell lines and CD138+ cells from patients as well as in murine xenograft model. Through siRNA silencing approach, we further confirmed that DPP8 but not DPP9 is a key molecule in inducing cell death induced by DPP8/9 inhibition. In fact, the expression of DPP8 in CD38+ cells from myeloma patients was higher than that of healthy volunteers. DPP8/9 inhibition induced apoptosis, as evidenced by activated form of PARP, caspases-3 and was suppressed by the pan-caspase inhibitor Z-VAD-FMK. Taken together, these results indicate that DPP8 is a novel therapeutic target for myeloma treatment.
Protective effects of Salidroside on cardiac function in mice with myocardial infarction
Authors: Chen Pengsheng , Liu Jia , Ruan Hongyun , Zhang Miaomiao , Wu Peng , Yimei Du , Han Bing
Published: December 2019

Salidroside (SAL) is the major ingredient of Rhodiola rosea, and has been traditionally used in Chinese medicine for decades. Numerous studies have demonstrated the protective effects of SAL for myocardial ischemia. However, it is yet to be deciphered whether SAL has cardioprotective effects after myocardial infarction (MI) in vivo. In the present study, we established a mouse MI model via coronary artery ligation. The aim was to investigate whether SAL treatment could reduce mortality, improve cardiac function and attenuate myocardial remodeling in MI mice. Post-surgery, mice were randomly administered SAL or normal saline. After 21 days, SAL was found to significantly reduce mortality, improve cardiac function, reduce fibrosis and infarct size compared to normal saline. In addition, oral administration of SAL could attenuate myocardial inflammation and apoptosis and promote angiogenesis. SAL down-regulated the expression levels of TNF-α, TGF-β1, IL-1β, Bax and up-regulate the expression of Bcl-2, VEGF, Akt and eNOS. These results indicated that SAL could alleviate the pathological processes of myocardial remodeling in MI mice, and may be a potentially effective therapeutic approach for the management of clinical ischemic cardiovascular diseases.
Effects of temperature and superparasitism on quality and characteristics of thelytokous Wolbachia-infected Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae) during mass rearing
Authors: Zhou Jin-Cheng , Li Yuan-Yuan , Liu Quan-Quan , Ning Su-Fang , Che Wu-Nan , Cong Bin , Dong Hui
Published: December 2019

ThelytokousWolbachia-infectedTrichogrammaspp. are widely used egg parasitoids against lepidopteran pests in biological control programs.Wolbachiamay manipulate host wasps for superparasitism and is sensitive to temperature. To explore effects of temperature and superparasitism, we compared fitness parameters andWolbachia-mediated phenotype of thelytokousWolbachia-infectedTrichogramma dendrolimibetween those emerging from superparasitised or single-parasitised hosts at 17, 21, 25, or 29 °C. Infected mothers ofT. dendrolimishowed reduced superparasitism and parasitism increased with temperature.Wolbachiatitre decreased with temperature when females emerged from singly-parasitised hosts, but there was no correlation in superparasitised hosts. Females showed higherWolbachiatitres at 21, 25, or 29 °C when developing from superparasitised hosts. The daily male ratio of offspring increased with temperature, and the day-age threshold for 5%, 50%, or 95% daily male ratio decreased with temperature in both parasitism forms. Females that emerged from superparasitised hosts had a shorter life span and reduced fecundity. These results indicate thatWolbachiamay affect host behaviour by increasing superparasitism to enhance its spread, but this has negative effects on thelytokousWolbachia-infectedT. dendrolimi.
Fabrication of porous NiMn2O4 nanosheet arrays on nickel foam as an advanced sensor material for non-enzymatic glucose detection
Authors: Zhang Jie , Sun Yudong , Li Xianchun , Xu Jiasheng
Published: December 2019

In this work, porous NiMn2O4 nanosheet arrays on nickel foam (NiMn2O4 NSs@NF) was successfully fabricated by a simple hydrothermal step followed by a heat treatment. Porous NiMn2O4 NSs@NF is directly used as a sensor electrode for electrochemical detecting glucose. The NiMn2O4 nanosheet arrays are uniformly grown and packed on nickel foam to forming sensor electrode. The porous NiMn2O4 NSs@NF electrode not only provides the abundant accessible active sites and the effective ion-transport pathways, but also offers the efficient electron transport pathways for the electrochemical catalytic reaction by the high conductive nickel foam. This synergy effect endows porous NiMn2O4 NSs@NF with excellent electrochemical behaviors for glucose detection. The electrochemical measurements are used to investigate the performances of glucose detection. Porous NiMn2O4 NSs@NF for detecting glucose exhibits the high sensitivity of 12.2 mA mM−1 cm−2 at the window concentrations of 0.99–67.30 μM (correlation coefficient = 0.9982) and 12.3 mA mM−1 cm−2 at the window concentrations of 0.115–0.661 mM (correlation coefficient = 0.9908). In addition, porous NiMn2O4 NSs@NF also exhibits a fast response of 2 s and a low LOD of 0.24 µM. The combination of porous NiMn2O4 nanosheet arrays and nickel foam is a meaningful strategy to fabricate high performance non-enzymatic glucose sensor. These excellent properties reveal its potential application in the clinical detection of glucose.
Deconstructing and repurposing the light-regulated interplay between Arabidopsis phytochromes and interacting factors
Authors: Golonka David , Fischbach Patrick , Jena Siddhartha G. , Kleeberg Julius R. W. , Essen Lars-Oliver , Toettcher Jared E. , Zurbriggen Matias D. , Möglich Andreas
Published: December 2019

Phytochrome photoreceptors mediate adaptive responses of plants to red and far-red light. These responses generally entail light-regulated association between phytochromes and other proteins, among them the phytochrome-interacting factors (PIF). The interaction with Arabidopsis thaliana phytochrome B (AtPhyB) localizes to the bipartite APB motif of the A. thaliana PIFs (AtPIF). To address a dearth of quantitative interaction data, we construct and analyze numerous AtPIF3/6 variants. Red-light-activated binding is predominantly mediated by the APB N-terminus, whereas the C-terminus modulates binding and underlies the differential affinity of AtPIF3 and AtPIF6. We identify AtPIF variants of reduced size, monomeric or homodimeric state, and with AtPhyB affinities between 10 and 700 nM. Optogenetically deployed in mammalian cells, the AtPIF variants drive light-regulated gene expression and membrane recruitment, in certain cases reducing basal activity and enhancing regulatory response. Moreover, our results provide hitherto unavailable quantitative insight into the AtPhyB:AtPIF interaction underpinning vital light-dependent responses in plants.
Two-dimensional layered lanthanide diphosphonates: synthesis, structures and sensing properties toward Fe3+ and Cr2O72−
Authors: Hou Xiaomin , Yan Chong-Chong , Xu Xiuling , Liang Ai-Qin , Song Zu-Wei , Tang Si-Fu
Published: December 2019

Four new lanthanide phosphonates with two-dimensional layered crystal structures have been synthesized and investigated for the optical sensing of Fe3+ and Cr2O72− ions.
Promoting effects of water on the NH3-SCR reaction over Cu-SAPO-34 catalysts: transient and permanent influences on Cu species
Authors: Wan Yuyi , Yang Guangpeng , Xiang Jinyao , Shen Xiaoqiang , Yang Dafei , Chen Yanrong , Rac Vladislav , Rakic Vesna , Du Xuesen
Published: December 2019

After water treatment, the unanchored ions transfer to more stable sites to produce active sites, creating a permanent influence, while the water promotion of the re-oxidation half-cycle is transient.
Copper-based redox shuttles supported by preorganized tetradentate ligands for dye-sensitized solar cells
Authors: Rodrigues Roberta R. , Lee Joseph M. , Taylor Natalie S. , Cheema Hammad , Chen Lizhu , Fortenberry Ryan C. , Delcamp Jared H. , Jurss Jonah W.
Published: December 2019

Three copper redox shuttles featuring tetradentate ligands were synthesized and evaluated computationally, electrochemically, and in dye-sensitized solar cell devices using a benchmark organic dye, Y123.
Microfluidics of binary liquid mixtures with temperature-dependent miscibility
Authors: Fornerod Maximiliano J. , Amstad Esther , Guldin Stefan
Published: December 2019

We report on the use of temperature to actively control the microfluidic mixing, phase separation and flow patterns of partially miscible binary liquid mixtures.
Comprehensive and comparative exploration of the Atp7b−/− mouse plasma proteome
Authors: Lacombe Maud , Jaquinod Michel , Belmudes Lucid , Couté Yohann , Ramus Claire , Combes Florence , Burger Thomas , Mintz Elisabeth , Barthelon Justine , Leroy Vincent , Poujois Aurélia , Lachaux Alain , Woimant France , Brun Virginie
Published: December 2019

Wilson's disease (WD), a rare genetic disease caused by mutations in the ATP7B gene, is associated with altered expression and/or function of the copper-transporting ATP7B protein, leading to massive toxic accumulation of copper in the liver and brain. The Atp7b−/− mouse, a genetic and phenotypic model of WD, was developed to provide new insights into the pathogenic mechanisms of WD. Many plasma proteins are secreted by the liver, and impairment of liver function can trigger changes to the plasma proteome. High standard proteomics workflows can identify such changes. Here, we explored the plasma proteome of the Atp7b−/− mouse using a mass spectrometry (MS)-based proteomics workflow combining unbiased discovery analysis followed by targeted quantification. Among the 367 unique plasma proteins identified, 7 proteins were confirmed as differentially abundant between Atp7b−/− mice and wild-type littermates, and were directly linked to WD pathophysiology (regeneration of liver parenchyma, plasma iron depletion, etc.). We then adapted our targeted proteomics assay to quantify human orthologues of these proteins in plasma from copper-chelator-treated WD patients. The plasma proteome changes observed in the Atp7b−/− mouse were not confirmed in these samples, except for alpha-1 antichymotrypsin, levels of which were decreased in WD patients compared to healthy individuals. Plasma ceruloplasmin was investigated in both the Atp7b−/− mouse model and human patients; it was significantly decreased in the human form of WD only. In conclusion, MS-based proteomics is a method of choice to identify proteome changes in murine models of disrupted metal homeostasis, and allows their validation in human cohorts.
Liquid-phase growth and optoelectronic properties of two-dimensional hybrid perovskites CH3NH3PbX3 (X = Cl, Br, I)
Authors: Bai Yu , Zhang Haixin , Zhang Mingjing , Wang Di , Zeng Hui , Zhao Jun , Xue Hao , Wu Guozheng , Su Jing , Xie Ying , Zhang Yuxia , Jing Hao , Yu Haohai , Hu Zhanggui , Peng Ruwen , Wang Mu , Wu Yicheng
Published: December 2019

A bottom-up route to fabricate molecularly thin CH3NH3PbX3 (X = Cl, Br, I) perovskite 2D crystals has been discovered and is very promising for superior new optoelectronic devices.
Nitroxide-mediated polymerisation of thioacrylates and their transformation into poly(acrylamide)s
Authors: Aksakal Suzan , Liu Renjie , Aksakal Resat , Becer C. Remzi
Published: December 2019

Nitroxide mediated polymers of thioacrylates can be transformed into poly(acrylamide)s.
Polybenzimidazole co-polymers: their synthesis, morphology and high temperature fuel cell membrane properties
Authors: Kumar B. Satheesh , Sana Balakondareddy , Unnikrishnan G. , Jana Tushar , Kumar K. S. Santhosh
Published: December 2019

Polybenzimidazole (PBI) random co-polymers containing alicyclic and aromatic backbones were synthesized using two different dicarboxylic acids (viz., cyclohexane dicarboxylic acid and terephthalic acid) by varying their molar ratios.
Thermo- and redox-responsive dumbbell-shaped copolymers: from structure design to the LCST–UCST transition
Authors: Zou Hui , Wu Qiliang , Li Qianwei , Wang Chunyao , Zhou Li , Hou Xiao-Hua , Yuan Weizhong
Published: December 2019

Redox- and thermo-responsive dumbbell-shaped copolymers and their self-assembly and stimuli-responsive properties were investigated.
Influence of uniform magnetic field on physicochemical properties of freeze-thawed avocado puree
Authors: Tan Yinying , Jin Yamei , Yang Na , Wang Zhe , Xie Zhengjun , Xu Xueming , Jin Zhengyu , Liao Xiaojun , Sun Han
Published: December 2019

3D magnetic fields have the potential to improve the quality of food after freeze-thawing.
Scalable lignin/graphite electrodes formed by mechanochemistry
Authors: Liu Lianlian , Solin Niclas , Inganäs Olle
Published: December 2019

Scalable and low cost lignin/graphite hybrid material electrodes formed by mechanochemistry.
A novel ratiometric fluorescent probe for rapid detection of hydrogen peroxide in living cells
Authors: Hu Linan , Liu Jiayi , Zhang Jie , Zhang Hailiang , Xu Pengfei , Chen Zhu , Xiao Enhua
Published: December 2019

A new ratiometric fluorescent probe JNY-1 for sensitive detection of H2O2 is presented with selectivity over other reactive oxygen species, reactive nitrogen species, and biologically relevant species. Imaging of H2O2 in liver cancer HepG2 cells was achieved.
A flexible-molecule force field to model and study hexanitrohexaazaisowurtzitane (CL-20) – polymorphism under extreme conditions
Authors: Bidault X. , Chaudhuri S.
Published: December 2019

The quantum-chemistry based force field developed by Smith and Bharadwaj is transferred to hexanitrohexaazaisowurtzitane (CL20), revealing pressure-induced alterations of ε-CL20.
Rapid and catalyst free synthesis of new bis(benzo[g]chromene) and bis(pyrano[3,2-c]chromene) derivatives and optimization of reaction conditions using response surface methodology
Authors: Hosseini Fahimeh Sadat , Bayat Mohammad , Afsharnezhad Milad
Published: December 2019

One-pot synthesis of C2-symmetric chromene derivatives, which serve as building blocks to prepare liquid crystals with potential electrical or optical properties.
A simple one-pot fabrication of silver loaded semi-interpenetrating polymer network (IPN) hydrogels with self-healing and bactericidal abilities
Authors: Ginting Mimpin , Masmur Indra , Pasaribu Subur P. , Hestina Hestina
Published: December 2019

The semi-interpenetrating polymer network hydrogel possesses self-healing ability due to the dynamic ionic interactions between polyacrylic acid and Fe3+. The antibacterial properties are due to embedded silver chloride nanoparticles well-distributed in the hydrogel.
The cotreatment of landfill leachate and high-nitrate wastewater using SBRs: evaluation of denitrification performance and microbial analysis
Authors: Liu Huaguang , Zhou Xingyu , Zhang Chaoshen , Zhang Jinsong
Published: December 2019

Resourceful disposal of landfill leachate has always been an intractable worldwide problem.
Environmentally benign, facile and selective recovery of gold from aqueous media: synergic role of carbon dots as green reductant and sensor towards Au3+ ions
Authors: Raji Kaviyarasan , Vadivel Ramanan , Thiyagarajan Senthil Kumar , Ramamurthy Perumal
Published: December 2019

We investigated N,S-CDs as a potential candidate for selective detection and extraction of Au3+ in aqueous media.
Persea americanaseed extract mediated gold nanoparticles for mercury(ii)/iron(iii) sensing, 4-nitrophenol reduction, and organic dye degradation
Authors: Bogireddy Naveen Kumar Reddy , Agarwal Vivechana
Published: December 2019

Gold nanoparticles for highly sensitive detection of Hg(ii)/Fe(iii) and catalytic efficiency towards the reduction/degradation of 4-nitrophenol and organic dyes.
Design and synthesis of hierarchical NiO/Ni3V2O8 nanoplatelet arrays with enhanced lithium storage properties
Authors: Li Yang , Duan Feng , Yang Shuai , Deng Qihuang , Liu Songli , Peng Cheng
Published: December 2019

Hierarchical NiO/Ni3V2O8 nanoplatelet arrays (NPAs) grown on Ti foil were prepared as free-standing anodes for Li-ion batteries (LIBs) via a simple one-step hydrothermal approach followed by thermal treatment to enhance Li storage performance.
Ternary dual Z-scheme graphitic carbon nitride/ultrathin metal–organic framework nanosheet/Ag3PO4 photocatalysts for boosted photocatalytic performance under visible light
Authors: Kusutaki Tomoharu , Katsumata Hideyuki , Tateishi Ikki , Furukawa Mai , Kaneco Satoshi
Published: December 2019

The enhanced photocatalytic activity of CN10UA results from fast charge transport through dual Z-scheme channels.
TiO2doped chitosan/hydroxyapatite/halloysite nanotube membranes with enhanced mechanical properties and osteoblast-like cell response for application in bone tissue engineering
Authors: Khan Sarim , Kumar Viney , Roy Partha , Kundu Patit Paban
Published: December 2019

This two-stage study aims to optimize the amount of halloysite nanotubes and TiO2in a chitosan/nano-hydroxyapatite composite to tailor the mechanical and biological properties for application in bone tissue engineering.
Enhancement of NH3-SCR performance of LDH-based MMnAl (M = Cu, Ni, Co) oxide catalyst: influence of dopant M
Authors: Du Yali , Liu Lili , Feng Yalin , Yang Baoshuan , Wu Xu
Published: December 2019

In situ doping of M (M = Cu, Ni, Co) into MnAl-LDH laminate to promote the NH3-SCR performance of the MnAl-LDO catalyst.
Synthesis of a novel hexaazatriphenylene derivative for the selective detection of copper ions in aqueous solution
Authors: Saeed Tahseen S. , Maddipatla Dinesh , Narakathu Binu B. , Albalawi Sarah S. , Obare Sherine O. , Atashbar Massood Z.
Published: December 2019

A hexaazatriphenylene (HAT) derivative, naphtho[2,3-h]naphtho[2′,3′:7,8]quinoxalino[2,3-a]naphtho[2′,3′:7,8]quinoxalino[2,3-c]phenazine-5,10,15,20,25,30-hexaone (NQH) was synthesized, characterized, and found to be selective to copper (Cu2+) ions.
Synthesis, cytotoxicity and anti-inflammatory activity of rhamnose-containing ursolic and betulinic acid saponins
Authors: Sylla Balla , Lavoie Serge , Legault Jean , Gauthier Charles , Pichette André
Published: December 2019

Rhamnose-containing saponins featuring betulinic and ursolic acid as aglycones were synthesized using both Schmidt's normal and inverse procedures. Some of these synthetic saponins exhibited selective cytotoxic and/or anti-inflammatory activities.
Surface structure-dependent electrocatalytic reduction of CO2 to C1 products on SnO2 catalysts
Authors: Fang Minling , Zheng Zhiping , Chen Jiayu , Chen Qian , Liu Deyu , Xu Binbin , Wu Jianyang , Kuang Qin , Xie Zhaoxiong
Published: December 2019

Surface structure-dependent electrocatalytic reduction of CO2 to C1 products on SnO2 catalysts was attributed to the in situ formation of different Sn/SnO2 catalytic layers on their surfaces.
Modifying perovskite solar cells with l(+)-cysteine at the interface between mesoporous TiO2 and perovskite
Authors: Wu Pengfei , Ma Xiaotong , Zhao Baohua , Liu Chengben , Chen Yanli , Yang Guangwu , Li Xiyou
Published: December 2019

A typical trifunctional amino acid, l(+)-cysteine, is employed to modify the interface between mesoporous TiO2 and perovskite film, and promotes the power conversion efficiency of perovskite solar cells from 11.5% to 14.4%.
Self-assembly of lobed particles into amorphous and crystalline porous structures
Authors: Paul Sanjib , Vashisth Harish
Published: December 2019

We report simulation studies on the self-assembly of hard-lobed particles of different shapes and show that various types of self-assembled morphologies can be achieved by tuning inter-lobe interactions.
In situ decorated Ni2P nanocrystal co-catalysts on g-C3N4 for efficient and stable photocatalytic hydrogen evolution via a facile co-heating method
Authors: Wen Peng , Zhao Kefei , Li Hui , Li Jiangsheng , Li Jing , Ma Qing , Geyer Scott M. , Jiang Lin , Qiu Yejun
Published: December 2019

A Ni2P/g-C3N4 hybrid photocatalyst were in situ fabricated via a one-step co-heating solution approach. The integrated photocatalyst demonstrated extraordinary H2 evolution and excellent durability due to the unique Ni(δ+)–N(δ−) chemical bonding.
A hybrid solid electrolyte Li0.33La0.557TiO3/poly(acylonitrile) membrane infiltrated with a succinonitrile-based electrolyte for solid state lithium-ion batteries
Authors: Bi Jiaying , Mu Daobin , Wu Borong , Fu Jiale , Yang Hao , Mu Ge , Zhang Ling , Wu Feng
Published: December 2019

An LLTO/PAN/SNE hybrid solid electrolyte membrane with high ionic conductivity and excellent compatibility with both LiFePO4 cathode and metallic lithium anode.