bluuebunny/crossref_metadata_2025 huggingface dataset:
Muscle as an osteoinductive niche for local bone formation with the use of a biphasic calcium sulphate/hydroxyapatite biomaterial
Authors: Raina D. B. , Gupta A. , Petersen M. M. , Hettwer W. , McNally M. , Tägil M. , Zheng M-H. , Kumar A. , Lidgren L.
Published: October 2016

Objectives

We have observed clinical cases where bone is formed in the overlaying muscle covering surgically created bone defects treated with a hydroxyapatite/calcium sulphate biomaterial. Our objective was to investigate the osteoinductive potential of the biomaterial and to determine if growth factors secreted from local bone cells induce osteoblastic differentiation of muscle cells.

Materials and Methods

We seeded mouse skeletal muscle cells C2C12 on the hydroxyapatite/calcium sulphate biomaterial and the phenotype of the cells was analysed. To mimic surgical conditions with leakage of extra cellular matrix (ECM) proteins and growth factors, we cultured rat bone cells ROS 17/2.8 in a bioreactor and harvested the secreted proteins. The secretome was added to rat muscle cells L6. The phenotype of the muscle cells after treatment with the media was assessed using immunostaining and light microscopy.

Results

C2C12 cells differentiated into osteoblast-like cells expressing prominent bone markers after seeding on the biomaterial. The conditioned media of the ROS 17/2.8 contained bone morphogenetic protein-2 (BMP-2 8.4 ng/mg, standard deviation (sd) 0.8) and BMP-7 (50.6 ng/mg, sd 2.2). In vitro, this secretome induced differentiation of skeletal muscle cells L6 towards an osteogenic lineage.

Conclusion

Extra cellular matrix proteins and growth factors leaking from a bone cavity, along with a ceramic biomaterial, can synergistically enhance the process of ectopic ossification. The overlaying muscle acts as an osteoinductive niche, and provides the required cells for bone formation. Cite this article: D. B. Raina, A. Gupta, M. M. Petersen, W. Hettwer, M. McNally, M. Tägil, M-H. Zheng, A. Kumar, L. Lidgren. Muscle as an osteoinductive niche for local bone formation with the use of a biphasic calcium sulphate/hydroxyapatite biomaterial. Bone Joint Res 2016;5:500–511. DOI: 10.1302/2046-3758.510.BJR-2016-0133.R1.
Mandibles of Leaf-Cutting Ants: Morphology Related to Food Preference
Authors: Camargo Roberto Da Silva , Silva Lais , Forti Luiz Carlos , Lopes Juliane Floriano
Published: October 2016

Feed adaptation is crucial for the ecological success of animals, which explore specific or varied resources according to the suitability of the selected feature. We hypothesized that the mandibles of leaf-cutting ants exhibit a greater degree of specialization because of the specific food preference of some species. The objective of this study was to highlight possible morphological differences between the mandibles of leaf-cutting ants of the genus Atta related to the food preference of the species, i.e., to investigate the morphofunctionality of mandibles in conjunction with a study of the leaf anatomy of plants selected. A detailed description of the mandibles of workers of the grass-cutting ant Atta bisphaerica and of the leaf-cutting ant Atta sexdens rubropilosa was provided, and the anatomy and chemical composition of the plant material foraged by these species were investigated. For morphometric analysis, 50 individuals of each of the four size classes were dissected for removal of the right mandible and one sample of each caste was processed for scanning electron microscopy. The Atta species had a similar mandibular morphology and differences were only observed between species and among castes, demonstrating the clear specialization of workers. Together with the anatomical study of the foraged plant material, the results indicate a high abundance of lignified cells in grasses, permitting to infer that the foraging and processing of these plants can lead to differences in behavioral acts during their preparation and incorporation in the fungus garden. We accept the hypothesis of morphofunctionality in view of the mandibular morphology observed.
A Simple Case of Pallet Setup Features Based Order Picking Routing Optimization
Authors: Bódis T , Botzheim J , Földesi P
Published: October 2016

Order picking is the most labour-intensive and costly activities in many warehouses by consuming ca. 55 % of the total operating expenses. Order picking development strategies mostly concentrate on warehouse layout, storage assignment policy, routing, zoning and on batching methods. The main challenges of order picking process improvements are the synchronization of these fields and fit the system with the further influencing factors, like unique demands and product parameters. Researchers of the pallet-loading problem could provide a wider horizon on considerable parameters, but their results are rarely implemented into order picking processes. The physical parameters of the products also have a significant impact on the processes which could strongly influence the picking sequence. \\The aim of this paper is to describe our own developed methodology for modeling the pallet setup rules. We introduce our product classifying and PSF based decision matrix modeling solutions. These will be the basis of our further research on PSF based harmonized routing and SLA optimization. We examine the complexity of a simple pallet setup features based order picking routing case, where the products of the defined pallet setup classes stored in separated zones.
The importance of organizing activities of the Turkish Cypriot Community in the process of becoming a state (1957-1960)
Authors: Koyuncu Mevlüt , Balıkçıoğlu Emrah
Published: October 2016

In this study, organization process of the Turkish Cypriot Community between 1957 and 1960 was examined. This study claims that this process was important, because it formed the basis of Turkish Cypriots’ becoming a state.It can be said that there are two main views about organization process of Turkish Cypriots in researches which were examined for this study. Researchers such as Ahmet An and Niyazi Kızılyürek allege that this process was Turco-British collusion to divide Turkish and Greek Cypriots who had lived together peacefully for centuries. According to researchers such as Ahmet C. Gazioğlu and Ulvi Keser; Turkish Cypriots started an organization process to protect themselves from Greek Cypriots’ hostility and attacks.  However, it seems that the relationship between organization process of the Turkish Cypriot Community and Turks’ becoming a state has not been handled yet.After the Great Britain took over the rule of Cyprus from the Ottoman Empire by the Cyprus Convention in 1878, the British established a Legislative Council. Greek Cypriots who desired to realize enosis (union with Greece) made attempts to pass enosis memorandums in the Council. On the contrary, Turkish Cypriots who saw union with Greece as a threat to their safety avoided these attempts with help of the British. Moreover, Turks published various newspapers and journals to voice their objections to enosis. Both these efforts and anti-Turkish feelings caused Greek Cypriots’ attacking Turkish Cypriots. In order to protect themselves from these attacks and conduct effective struggle against enosis, Turkish Cypriots set up assemblies, political parties, farmer and trade unions, social associations and armed organizations from 1918 to 1957. However, it was 1957 when these organization activities became continuous process. Hence the Turkish Cypriot Community was organized in terms of, military, administration, economy and culture during the period between 1957 and 1960. Turkish states which were founded between 1964 and 1983 were based on these organizations.   Considering this fact, the study is composed of two main parts. The first part summarizes the organization efforts of the Turkish Cypriot Community between 1918 and 1957. The second part focuses on the organization process of the Turkish Cypriot Community between 1957 and 1960.
Effects of tuber size, soaking hours and sprouting media on sprouting of tiger nut (Cyperus esculentus L. var. sativa) tubers
Authors: Fabunmi Thomas Oladeji , Adigbo Sunday Ojo , Odedina Joy Nwakaego , Akinsanya Yanmife Iyanu
Published: October 2016

Tiger nut, Cyperus esculentus L., is one of the underutilized and neglected food crops in most parts of the world leading to limited information on its production technology. A trial arranged in a 3×4×2 factorial of a completely randomized design was conducted in a green house of the Federal University of Agriculture, Abeokuta, Nigeria, to evaluate the effects of tuber size (large, medium and small), soaking duration (0, 24, 48 and 72 hours) and sprouting media (cotton wool and polythene bag) on the sprouting of tiger nut. Significant interactions were observed between growth media and soaking hours as well as between tuber sizes and soaking hours on the sprouting of tiger nut. Soaking beyond 24 hours before placement in sprouting medium led to a significant reduction in number of sprouted tubers in polythene bags. Small sized tubers had reduced (p<0.05) number of sprouts when soaked beyond 24 hours. Sprouting of tiger nut was better using medium size tubers soaked for 24 hours and placed between layers of cotton wool.
The effect of endurance exercise on intestinal integrity in well‐trained healthy men
Authors: JanssenDuijghuijsen Lonneke M. , Mensink Marco , Lenaerts Kaatje , Fiedorowicz Ewa ,   the Protégé study group, van Dartel Dorien A. M. , Mes Jurriaan J. , Luiking Yvette C. , Keijer Jaap , Wichers Harry J. , Witkamp Renger F. , van Norren Klaske
Published: October 2016

Exercise is one of the external factors associated with impairment of intestinal integrity, possibly leading to increased permeability and altered absorption. Here, we aimed to examine to what extent endurance exercise in the glycogen‐depleted state can affect intestinal permeability toward small molecules and protein‐derived peptides in relation to markers of intestinal function. Eleven well‐trained male volunteers (27 ± 4 years) ingested 40 g of casein protein and a lactulose/rhamnose (L/R) solution after an overnight fast in resting conditions (control) and after completing a dual – glycogen depletion and endurance – exercise protocol (first protocol execution). The entire procedure was repeated 1 week later (second protocol execution). Intestinal permeability was measured as L/R ratio in 5 h urine and 1 h plasma. Five‐hour urine excretion of betacasomorphin‐7 (BCM7), postprandial plasma amino acid levels, plasma fatty acid binding protein 2 (FABP‐2), serum pre‐haptoglobin 2 (preHP2), plasma glucagon‐like peptide 2 (GLP2), serum calprotectin, and dipeptidylpeptidase‐4 (DPP4) activity were studied as markers for excretion, intestinal functioning and recovery, inflammation, and BCM7 breakdown activity, respectively. BCM7 levels in urine were increased following the dual exercise protocol, in the first as well as the second protocol execution, whereas 1 h‐plasma L/R ratio was increased only following the first exercise protocol execution. FABP2, preHP2, and GLP2 were not changed after exercise, whereas calprotectin increased. Plasma citrulline levels following casein ingestion (iAUC) did not increase after exercise, as opposed to resting conditions. Endurance exercise in the glycogen depleted state resulted in a clear increase of BCM7 accumulation in urine, independent of DPP4 activity and intestinal permeability. Therefore, strenuous exercise could have an effect on the amount of food‐derived bioactive peptides crossing the epithelial barrier. The health consequence of increased passage needs more in depth studies.
Removal of copper from copper-contaminated river water and aqueous solutions using Methylobacterium extorquens modified Erzurum clayey soil
Authors: Celebi Neslihan , Nadaroglu Hayrunnisa , Kalkan Ekrem , Kotan Recep
Published: October 2016

The objective of this study was to investigate the possibility of using natural and bacteria-modified Erzurum clayey soil with Methylobacterium extorquens as an alternative to high cost commercial adsorbent materials for the removal of copper from aqueous solution. The copper concentrations in the samples of the polluted river water and CuCl2 solutions treated by the natural and bacteria-modified Erzurum clayey soil (ECS) have been determined by spectrophotometric method. Firstly, the surface of ECS was modified with M. extorquens and surface functionality was increased. Then, the adsorption of Cu (II) from solution phases was studied with respect to varying metal concentration, pH, and temperature and agitation time. The maximum adsorption of Cu (II) for natural and bacteria-modified Erzurum clayey soil was observed at pH: 5.0. At different copper concentrations, copper adsorption analysis was performed on 1 g using clay soil or modified clay soil. Maximum adsorption of Cu (II) was obtained as 45.7 and 48.1 mg g-1 at initial concentration (50 mg/50 mL) and optimal conditions by natural and bacteria-modified clay soil, respectively. The copper concentration was decreased in the substantial amount of the leachates solutions of natural and bacteria-modified clay soil. Langmuir and Freundlich isotherms were used to describe the adsorption behavior of Cu (II) ions. The results showed that modified clay soil had a high level of adsorption capacity for copper ion. The various thermodynamic parameters such as ΔG°, ΔH° and ΔS° were analyzed to observe the nature of adsorption. The structural properties of the natural and bacteria-modified-ECS have been characterized by SEM, FTIR and XRD techniques. Consequently, it was concluded that the bacteria-modified clay soil could be successfully used for the removal of the copper ions from the aqueous solutions.
The influence of hydraulic loading on biofilm properties in a subsurface wastewater infiltration system
Authors: Zhang Liangbo , Yang Jian
Published: October 2016

In this study, a pilot-scale subsurface wastewater infiltration system (SWIS) was deployed to study landscape water treatment. The goal of the study was to investigate the effects of hydraulic loading on pollutant removal and the spatial distribution of biofilm properties in SWIS. Results showed that the efficiencies of chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) removal degraded as hydraulic loading increased. Furthermore, quantities of the biofilm properties parameter s increased with the hydraulic loading. Polysaccharide and protein levels ranged from 560 to 1110 μg/g filler and 60 to 190 μg/g filler, respectively, at a hydraulic loading of 0.2 m/d. At a hydraulic loading of 0.4 m/d, the quantities of polysaccharide and protein ranged from 1200 to 3300 μg/g filler and 80 to 290 μg/g filler, respectively. Biofilm intensity and biofilm activity per unit weight decreased with the increase in hydraulic loading.
Content of phenolic compounds in soils originating from two long-term fertilization experiments
Authors: Sądej Wiera , Żołnowski Andrzej Cezary , Marczuk Olga
Published: October 2016

The objective of the study was to compare the impact of three systems of multiannual fertilization applied in two long-term field experiments on the content of phenolic compounds in the soil. In the study, both natural (manure, slurry) and mineral (NPK) fertilizers were used, along with combined, organic-and-mineral fertilization. Experiment I was established in 1972 on grey brown podzolic soil; experiment II, in 1973 on brown soil. In both experiments crops were cultivated in a 7-year rotation, with a 75% share of cereals. The experimental samples were taken from the top layer of soil after 36 (experiment I) and 35 (experiment II) years following the establishment of the experiments. It was demonstrated that the presence of phenolic compounds in the soils was significantly dependent on the contents of organic C and total N, type of soil and the type and dose of used fertilizers. In grey brown podzolic soil, the content of total phenolic compounds was at a lower level than the content found in brown soil. Multiannual fertilization contributed to an increase in the content of total phenolic compounds in relation to the values obtained in control objects, which was particularly reflected in the soil originating from objects fertilized with slurry applied at a dose being equivalent to manure in terms of the amount of introduced organic carbon. The percentage of water-soluble phenols in the total content of these compounds in grey brown podzolic soil was at the level of 18.4%, while in brown soil it amounted to 29.1%.
Norway rat (Rattus norvegicus) as a potential reservoir for Echinococcus granulosus: A public health implication
Authors: Abdel-Moein Khaled A. , Hamza Dalia A.
Published: October 2016

Cystic hydatidosis is a re-emerging parasitic zoonosis with worldwide distribution. The current study was carried out to investigate the possible role of rats in the epidemiology of such disease in urban and suburban areas. For this purpose, a total of 50 feral Norway rats (
The PCR and nested PCR detection of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Dermacentor reticulatus F. collected in a new location in Poland (Trzciel, Western Poland)
Authors: Opalińska Patrycja , Wierzbicka Anna , Asman Marek
Published: October 2016

The study was performed in the Trzciel Forest Districts which is located in the west part of Poland. The Scots pine is the main tree species creating forest landscape there.
Role of Superplasticizer Additives Upon Hydration Process of Cement Pastes
Authors: Angelescu Nicolae , Stanciu Darius , Barroso de Aguiar José , Abdelgader Hakim S. , Bratu Vasile
Published: October 2016

The article presents a comparative analysis on the hydration of cement paste without superplasticizer and water/cement ratio of 0.35 and a cement paste with the same water/cement ratio but has in its composition 2% superplasticizer additive Glenium Sky 526. For characterizing the hydration process of cement paste, both mixtures were subjected to X-ray diffraction and thermogravimetry analysis, at 3, 7, and 28 days passed since the initiation of hydration process.
Path Searching Based Fault Automated Recovery Scheme for Distribution Grid with DG
Authors: Xia Lin , Qun Wang , Hui Xue , Simeng Zhu
Published: October 2016

Applying the method of path searching based on distribution network topology in setting software has a good effect, and the path searching method containing DG power source is also applicable to the automatic generation and division of planned islands after the fault. This paper applies path searching algorithm in the automatic division of planned islands after faults: starting from the switch of fault isolation, ending in each power source, and according to the line load that the searching path traverses and the load integrated by important optimized searching path, forming optimized division scheme of planned islands that uses each DG as power source and is balanced to local important load. Finally, COBASE software and distribution network automation software applied are used to illustrate the effectiveness of the realization of such automatic restoration program.
Modelling of the soft X-ray tungsten spectra expected to be registered by GEM detection system for WEST
Authors: Syrocki Łukasz , Szymańska Ewa , Słabkowska Katarzyna , Polasik Marek , Pestka Grzegorz
Published: October 2016

In the future International Thermonuclear Experimental Reactor (ITER), the interaction between the plasma and the tungsten chosen as the plasma-facing wall material imposes that the hot central plasma loses energy by X-ray emission from tungsten ions. On the other hand, the registered X-ray spectra provide alternative diagnostics of the plasma itself. Highly ionized tungsten emits extremely complex X-ray spectra that can be understood only after exhaustive theoretical studies. The detailed analyses will be useful for proper interpretation of soft X-ray plasma radiation expected to be registered on ITER-like machines, that is, Tungsten (W) Environment in Steady-state Tokamak (WEST). The simulations of the soft X-ray spectra structures for tungsten ions have been performed using the flexible atomic code (FAC) package within the framework of collisional-radiative (CR) model approach for electron temperatures and densities relevant to WEST tokamak.
Estimating the unconfined yield strength of coal in the case of longwall coal mining with hanging wall top caving
Authors: Hann Damjan
Published: October 2016

This study presents an innovative approach for determining the unconfined yield strength σc during the excavation of coal from the earth’s crust by using an equipment that was developed for measuring the mechanical properties of bulk materials stored in silos. Highly productive excavation of coal with a hanging wall top caving leads to intensive deformations in the hanging wall and the broken coal can be considered as bulk material. In this research, the shear tester Johanson Hang-Up Indicizer was used to measure the unconfined yield strength of the tested samples, even though such a tester cannot produce stress-strain conditions similar to those occurring during the excavation. An attempt was made to estimate the real unconfined yield strength of broken coal deep under the surface through a combination of measured data and extrapolation.
Geo-electrical assessment of coefficient of permeability and porosity of leachate plume at Asin dumpsite, Iwaro-oka, southwestern Nigeria
Authors: Okpoli Cyril , Oladunjoye Michael , Ajayi Toluwaleke
Published: October 2016

An integrated geophysical (involving two-dimensional [2D] electrical resistivity) and petrophysical study was conducted in the Precambrian Crystalline Basement area of Iwaro-oka Akoko, southwestern Nigeria. Five 2D resistivity profiles, both around the perimeters and inside the dump, were investigated with maximum lengths of 100 m. Results of the resistivity imaging delineated the leachate plumes as low-resistivity zones, with values ranging from 3 Ω m to 55 Ω m. The coefficient of permeability ranged from 4.33 × 10-6 to 7.82 × 10-3, and the average porosity ranged from 32 Ω m to 169 Ω m, thus indicating migration of leachate plume to the groundwater due to the high coefficient of permeability and the porosity.
Geophysical investigation of banded iron ore mineralization at Ero, North – Central Nigeria
Authors: Oyedele Kayode , Oladele Sunday , Salami Anthony
Published: October 2016

The banded iron ore mineralization at Ero was investigated using aeromagnetic, resistivity and induced polarization (IP) methods with the aim of characterizing the deposit. Analysis of the aeromagnetic data involved the application of reduced-to-equator transformation, derivative filters, analytic signal and source parameter imaging techniques. Computer modelling of some of the identified anomalies was undertaken. The electrical resistivity and IP methods helped in discriminating between the iron ore and the host rock. The results showed that the banded iron formations (BIFs) were characterized by spherical analytic signal anomalies ranging from 0.035 nT/m to 0.06 nT/m within the granite gneiss and magnetic susceptibility of 0.007-0.014 SI. The iron ore had low chargeability (0.1-5.0 msec) and resistivity (1.5 × 102 to 2.5 × 103 Ωm). Structural features trending in the NE-SW, E-W, and NW-SE were identified, suggesting that the area had undergone many episodes of tectonic events. Depth to the BIF varied from the surface up to about 200 m. The chargeability response of the iron bodies suggested an average grade of 20%-40%, making the prospect for economic exploitation attractive.
Fitting the SEIR model of seasonal influenza outbreak to the incidence data for Russian cities
Authors: Leonenko Vasiliy N. , Ivanov Sergey V.
Published: October 2016

In this paper we present a computational algorithm aimed at fitting a SEIR populational model to the influenza outbreaks incidence in Russian cities. The input data are derived from the long-term records on the incidence of acute respiratory diseases in Moscow, St. Petersburg, and Novosibirsk. It is shown that the classical SEIR model could provide a satisfactory fit for the majority of employed influenza outbreak incidence data sets (
The study of scroll wave dynamics in personalized models of the left ventricle of the human heart
Authors: Ushenin Konstantin S. , Pravdin Sergei F. , Alueva Yuliya S. , Chumarnaya Tatyana V. , Solovyova Olga E.
Published: October 2016

This paper presents first results on the dynamics of filaments of scroll waves of myocardium excitation obtained for personalized models of the left ventricle of the human heart. The paper describes a mathematical model of the left ventricle of the human heart and its electrical activity, numerical methods for the model calculation within the framework of computer implementation, and also the method of personalization of the model according to data of ultrasound examination. We found that regardless of the starting point of wave the filaments of wave drift along a spiral to the attractor located near the apex of the ventricle. The attractor position was essentially different in models constructed from the data of patients without identified pathology and those for patients with an increased left ventricular cavity.
31P-Solid-State NMR Characterization and Catalytic Hydrogenation Tests of Novel heterogenized Iridium-Catalysts
Authors: Gutmann Torsten , Alkhagani Safaa , Rothermel Niels , Limbach Hans-Heinrich , Breitzke Hergen , Buntkowsky Gerd
Published: October 2016

The synthesis of novel robust and stable iridium-based immobilized catalysts on silica-polymer hybrid materials (Si-PB-Ir) is described. These catalysts are characterized by a combination of 1D31P CP-MAS and 2D31P-1H HETCOR and J-resolved multinuclear solid state NMR experiments. Different binding situations such as singly and multiply coordinated phosphines are identified. Density functional theory (DFT) calculations are performed to corroborate the interpretation of the experimental NMR data, in order to propose a structural model of the heterogenized catalysts. Finally, the catalytic activity of the Si-PB-Ir catalysts is investigated for the hydrogenation of styrene employing para-enriched hydrogen gas.
The diverse and expanding role of mass spectrometry in structural and molecular biology
Authors: Lössl Philip , van de Waterbeemd Michiel , Heck Albert JR
Published: October 2016

The emergence of proteomics has led to major technological advances in mass spectrometry (MS). These advancements not only benefitted MS‐based high‐throughput proteomics but also increased the impact of mass spectrometry on the field of structural and molecular biology. Here, we review how state‐of‐the‐art MS methods, including native MS, top‐down protein sequencing, cross‐linking‐MS, and hydrogen–deuterium exchange‐MS, nowadays enable the characterization of biomolecular structures, functions, and interactions. In particular, we focus on the role of mass spectrometry in integrated structural and molecular biology investigations of biological macromolecular complexes and cellular machineries, highlighting work on CRISPR–Cas systems and eukaryotic transcription complexes.
TECRL: connecting sequence to consequence for a new sudden cardiac death gene
Authors: Perry Matthew D , Vandenberg Jamie I
Published: October 2016

The sudden unexpected death of a child is a devastating event. One of the first questions a family will ask is “Why did this happen?” In some cases, the answer may become obvious during a postmortem examination, but in up to 40% of cases, the postmortem is negative (Bagnall et al, ). In the last 1–2 decades, an improved understanding of the genetic basis of the primary arrhythmia syndromes, the major cause of sudden unexplained death in children with structurally normal hearts, has greatly enhanced our ability to make a postmortem diagnosis (Van Norstrand & Ackerman, ). Establishing an accurate genetic diagnosis can not only answer the parents' question as to why did this happen to my child, but is invaluable for cascade screening of all family members to identify other individuals harbouring the same mutation and who therefore may be at risk of sudden cardiac death. However, even after screening for all of the established genes associated with primary arrhythmia syndromes, up to two thirds of unexplained cardiac deaths will remain unsolved. Such was the case for a family of Sudanese origin with a highly malignant form of exercise‐induced arrhythmias, originally reported by Bhuiyan et al ().
Teaching communications skills to medical students using a reflective teaching method and access to online video cases
Authors: Jorgensen Merete , Witt Klaus
Published: October 2016

This article was migrated. The article was not marked as recommended. Introduction In our family medicine course the students work eight days in a general practice clinic, video-record their consultations, and analyze them in small group sessions at the university. The aim of this study is to find a way to evaluate the course after adding online material. Material and Methods On the first and last day of the course all students watch the same test-video of a consultation. After watching the video they fill in a questionnaire, designed to structure the analysis. Outcome is the change in answers from the first questionnaire (A) to the last one (B).For the on-line teaching we use 16 short video-cases of consultations accompanied by on-line questions. The control group has the usual course, Results In seven out of ten items there are significant changes in the answers in the control group. The tendency is the same in the intervention group but not significant. Apart from one item the two groups finished at the same answers. Conclusion Teaching consultation skills to medical students using a reflective teaching method is effective. Adding access to video cases has a minor effect. Discussion Further studies into how to use e-learning material in teaching communication skills is needed.
Building condition and impact assessment of underground construction
Authors: Goh Kok Hun
Published: October 2016

The approach to assessing the risk of building damage due to underground construction in Singapore broadly follows a staged approach described in local guides such as the Land Transport Authority of Singapore’s Civil Design Criteria for Road and Rail Transit Systems and TR 26:2010. However, the current approach does not consider the existing condition of buildings prior to external effects from adjacent underground construction. This paper presents an experience in developing a method to incorporate the existing condition of buildings into the impact assessment framework. The first part of the paper discusses the work to develop guidance on classifying the existing condition of buildings more objectively. The second half of this paper explores various methods that incorporate the existing condition of buildings into the damage assessment framework. These have enhanced the objectivity of visual inspection works as well as giving more confidence on impact assessment studies conducted in Singapore.
Prospects for the cycling development in Ivano-Frankivsk
Authors: Levytska О.
Published: October 2016

Analysis of the status and prospects of development of cycling on an example of Ivano-Frankivsk, determining the place of cycling in the city transport concept and its role in urban planning as a whole. Done mobile SWOT-analysis of the situation in Ivano-Frankivsk. Presented and analyzed the planned location of bicycle paths in Ivano-Frankivsk to the implementation of program activities Disclosed long term development of cycling, and form the basis for the Master Plan Cycling.
Investigation the role of mutations M182T and Q39K in structure of beta-lactamase TEM-72 by molecular dynamics method
Authors: Shcherbinin D.S. , Rubtsova M.Yu. , Grigorenko V.G. , Uporov I.V. , Veselovsky A.V. , Egorov A.M.
Published: October 2016

Synthesis of b-lactamases is one of the common mechanisms of bacterial resistance to b-lactam antibiotics including penicillins and cephalosporins. The widespread use of antibiotics results in appearance of numerous extended-spectrum b-lactamase variants or resistance to inhibitors. Mutations of 92 residues of TEM type were found. Several mutations are the key mutations that determine the extension of spectrum of substrates. However, roles of the most associated mutations, located far from active site, remain unknown. We have investigated the role of associated mutations in structure of b-lactamase TEM-72, which contain two key mutation (G238S, E240K) and two associated mutations (Q39K, M182T) by means of simulation of molecular dynamics. The key mutation lead to destabilization of the protein globule, characterized by increased mobility of amino acid residues at high temperature of modelling. Mutation M182T lead to stabilization protein, whereas mutation Q39K is destabilizing mutation. It seems that the last mutation serves for optimization of conformational mobility of b-lactamase and may influence on enzyme activity.
Apoptotic endonuclease EndoG regulates alternative splicing of human telomerase catalytic subunit hTERT
Authors: Zhdanov D.D. , Vasina D.A. , Orlova E.V. , Orlova V.S. , Pokrovskaya M.V. , Aleksandrova S.S. , Sokolov N.N.
Published: October 2016

Human telomerase catalytic subunit hTERT is subjected to alternative splicing results in loss of its function and leads to decrease of telomerase activity. However, very little is known about the mechanism of hTERT pre-mRNA alternative splicing. Apoptotic endonuclease EndoG is known to participate this process. The aim of this study was to determine the role of EndoG in regulation of hTERT alternative splicing. Increased expression of b-deletion splice variant was determined during EndoG over-expression in CaCo-2 cell line, after EndoG treatment of cell cytoplasm and nuclei and after nuclei incubation with EndoG digested cell RNA. hTERT alternative splicing was induced by 47-mer RNA oligonucleotide in naked nuclei and in cells after transfection. Identified long non-coding RNA, that is the precursor of 47-mer RNA oligonucleotide. Its size is 1754 nucleotides. Based on the results the following mechanism was proposed. hTERT pre-mRNA is transcribed from coding DNA strand while long non-coding RNA is transcribed from template strand of hTERT gene. EndoG digests long non-coding RNA and produces 47-mer RNA oligonucleotide complementary to hTERT pre-mRNA exon 8 and intron 8 junction place. Interaction of 47-mer RNA oligonucleotide and hTERT pre-mRNA causes alternative splicing.
Best Practices and Innovations for Managing Codeine Misuse and Dependence
Authors: Norman Ian J. , Bergin Michael , Parry Charles D. , Van Hout Marie Claire
Published: October 2016

Purpose. Promoting and ensuring safe use of codeine containing medicines remains a public health issue given the rise in reporting of misuse and dependence particularly in countries where available over–the-counter (OTC). The aim of this unique study was to identify best practices in management of opioid abuse and dependence, particularly codeine, and innovations to meet challenges surrounding safe and compliant use, patient awareness-raising, reducing health harms and enhancing successful treatment of dependence. Methods. A mixed methods approach using three data points was used that included : (1) analysis of data from existing scoping reviews to identify potential areas for innovation (2) interviews with key national stakeholders from public health, pharmaceutical, regulatory, primary care and addiction practice in three distinct regulatory regimes (Ireland, United Kingdom and South Africa); and (3) a circular email request for information on potential innovations to members of the European Medicine’s Agency European Network of Centres for Pharmacoepidemiology and Pharmacovigilance (ENCEPP). Data from these three sources were analysed to identify best practices and opportunities for innovation. Results. Best practices and potential innovations were identified under the nine headings: (1) manufacture; (2) product information and public education; (3) responsible prescribing; (4) monitoring and surveillance; (5) dispensing, screening and brief interventions in community pharmacies; (6) safety in the workplace and on the road; (7) internet supply of codeine and online support; (8) treatment of codeine dependence; and (9) learning resources and training for health professionals. Conclusions. Challenges ensuring availability of codeine containing medicines for legitimate therapeutic use, while minimising misuse, dependence and related health harms warrant consideration of new innovations. Most promising innovative potential lies across the products’ retail lifecycle from manufacture to prescriber and community pharmacy practitioner.This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.
Pengaruh Penerapan Experiential Marketing terhadap Pelanggan di Rumah Makan Lesehan Joyo Taman Pinang Sidoarjo
Authors: Astuti Mudji , Gresivonda Gresivonda
Published: October 2016

Personal experiences help consumers to be linked with brand or product and company as well as inform their purchasing decisions. Conditions of experiential marketing are the actual consumer experience with brands, products or services that can increase sales, brand image and brand awareness through senses, feeling, think, act and relate. Telling consumers about the features and usability of a product or service is quite different with letting them get the experiences by using the product or service themselves. If experiential marketing could be applied in the marketing activity of a firm, then it can be a great tool to increase its brand loyalty.

This research is conducted by observing the influence of Experiential Marketing (EXEM) implementation to customer satisfaction at Joyo Restaurant in Taman Pinang Sidoarjo, both partially and simultaneously.

Five independent variables – such as sense, feel, think, act, and relate – are used in this research based on Strategic Experiental Modules (SEMs), with customer satisfaction as the dependent variable.  The data of 100 customers of the restaurant – as the samples – is analyzed with multiple regresion analysis.

Using the t test and F test to test the hypothesis at the 5% significance level, the result shows that sense, feel, think, act and relate significantly affect customer satisfaction.  With adjusted R square at 48,6%, this proves that customer satisfaction can be explained by the variables of  Strategic Experiental Modules (SEMs), while the remaining 51,4% is explained by other variables.
Persepsi Masyarakat terhadap Kualitas Pelayanan di Terminal Arjosari Kota Malang
Authors: Maskan Mohammad , Utaminingsih Alifiulahtin , Soepeno Bambang
Published: October 2016

This study aims to analyze the quality of service in Arjosari Bus Station Malang. The service quality theory used in the study refers to the opinion of Tjiptono (2000) which consists of: physical evidence (tangibility), reliability, responsiveness, assurance, and empathy. The study uses a descriptive approach, by taking a sample of 30 service users of Arjosari Bus Station Malang. While, the methods of analysis use the mean.

The results show that the quality of service in Arjosari Bus Station Malang is good enough with an average score of 2.86. However, to improve the quality of service to the service users of the bus station, then there are several things that must be done by the local government of Malang, ie: adding free WIFI facility in bus station, fixing the layout and the exterior rooms of the bus station, and improving the room's cleanliness and coolness at the bus station environment. Furthermore, it also improves the reliability in service, such as: the simplicity for the bus station service users to consult with officials and leaders where necessary to the provision of the excellent service.
Integrasi Pendidikan Karakter Dalam Pembelajaran IPS Di Sekolah Dasar
Authors: Afandi Rifki
Published: October 2016

Pendidikan pada dasarnya merupakan suatu upaya sadar untuk mengembangkan potensi peserta didik secara optimal. pendidikan karakter, yaitu; membentuk pribadi anak, supaya menjadi manusia yang baik, warga masyarakat, dan warga negara yang baik, sehingga mampu mengantisipasi gejala krisis moral dan berperan dalam rangka pembinaan generasi muda. Pendidikan IPS adalah mata pelajaran yang mengkaji kehidupan sosial yang bahannya didasarkan pada kajian sejarah, geografi, ekonomi, sosiologi, antropologi dan tata negara. Melalui pembelajaran ilmu pengetahuan sosial dapat di masukkan nilai-nilai pendidikan karakter dengen mengintegrasikan materi dalam pembelajaran ilmu pengetahuan sosial tersebut.
Tri Pusat Pendidikan Sebagai Sarana Pendidikan Karakter Anak Sekolah Dasar
Authors: Kurniawan Machful Indra
Published: October 2016

Globalisasi memberikan dampak positif dan negatif bagi setiap warga negara indonesia. namun, tidak setiap warga negara menyikapi dampak negatif globalisasi dengan baik. Terjadinya penurunan kuwalitas moral bangsa merupakan salah satu dampak negatif dari globalisasi.Pemerintah tampaknya sudah mulai sadar akan pentingnya  nilai moral bagi sebuah bangsa, hal tersebut dapat  terlihat dengan digalakannya pembangunan moral bangsa melalui pendidikan karakter dalam sistem pendidikan nasional sejak tahun 2010 hingga saat ini yang diintegrasikan dalam kurikulum 2013.Tri pusat pendidikan merupakan sarana yang tepat dalam membentuk karakter anak, terutama anak sekolah dasar. pembentukan karakter anak sekolah dasar dapat dilakukan dengan cara menanamkan nilai-nilai karakter secara konsisten baik ketika anak berada dalam lingkungan keluarga, lingkungan sekolah, maupun lingkungan masyarakat. Dengan adanya konsistensi tersebut, karakter yang diharapkan dapat tertananam dengan baik sehingga terbentuk kakrakter yang baik.
Deskripsi Hubungan Komunikasi dan Kecerdasan Emosional Mahasiswa Pendidikan Matematika dalam Memecahkan Masalah Matematis
Authors: Agustin Ririn Dwi
Published: October 2016

Setiap mahasiswa memiliki karakteristik yang berbeda, termasuk kemampuan komunikasi dan kecerdasan emosional. Salah satu tujuan pembelajaran matematika adalah agar mahasiswa memiliki kemampuan memecahkan masalah dan mengkomunikasikan gagasan dan ide matematika. Dosen sebaiknya memperhatikan kemampuan komunikasi dan kecerdasan emosional mahasiswa agar dapat merancang pembelajaran yang mendukung terciptanya komunikasi matematika. Penelitian ini membahas tentang hubungan komunikasi lisan dan kecerdasan emosional mahasiswa pendidikan matematika. Penelitian ini termasuk penelitian kuantitatif dengan menggunakan uji validitas, uji reabilitas dan uji korelasi. Dari data yang sudah diperoleh, karena p = 0,02 (< 0,05), dengan demikian Ho ditolak sehingga disimpulkan ada hubungan antara komunikasi matematis dengan kecerdasan emosional.
Pembelajaran Membaca Permulaan dengan Menggunakan Media Aplikasi Bamboomedia Bmgames Apps Pintar Membaca Sebagai Upaya Pembentukan Karakter Siswa SD Menghadapi MEA
Authors: Hasanudin Cahyo
Published: October 2016

Eror
Pengembangan Perangkat Pembelajaran PKn Berbasis Pembelajaran Aktif untuk Meningkatkan Hasil Belajar Siswa Sekolah Dasar
Authors: Kurniawan Machful Indra , Afandi Rifki
Published: October 2016

Penelitian ini bertujuan: (1) mengembangkan perangkat pembelajaran PKn berbasis pembelajaran aktif, (2) mendeskripsikan kualitas hasil pengembangan. (3) mendeskripsikan keterlaksanaan Rencana Pelaksanaan Pembelajaran (RPP) PKn selama kegiatan pembelajaran. (4) mengetahui peningkatan hasil belajar siswa SD setelah diterapkan. Penelitian ini merupakan penelitian pengembangan dengan menggunakan model pengembangan perangkat Dick & Carey. Objek penelitian adalah perangkat pembelajaran PKn dan subjek uji coba adalah siswa kelas IV SD N Tambakrejo II Gresik. Hasil penelitian yaitu: (1) Pengembangan perangkat pembelajaran dengan cara mengidentifikasi tujuan pembelajaran, menganalisis pembelajaran, mengalisis siswa, merumuskan tujuan pembelajaran, menyusunan tes beracuan kriteria, mengembangkan strategi pembelajaran, memilih media pembelajaran, mengembangkan perangkat pembelajaran, dan melaksanakan validasi. (2) Kualitas perangkat pembelajaran diperoleh berdasarkan hasil validasi para ahli yaitu silabus, RPP, LKS, dan BAS yang memiliki kategori sangat baik. (3) Keterlaksanaan RPP pada uji coba 1 dan uji coba 2 dalam 3 pertemuan memiliki kategori rata-rata sangat baik. (4) Hasil uji coba pengembangan perangkat pembelajaran PKn SD pada uji coba 1 dan uji coba 2 terjadi peningkatan hasil belajar siswa sebesar 21%.
Kesulitan Mahasiswa dalam Mencapai Pembelajaran Bahasa Inggris Secara Efektif
Authors: Megawati Fika
Published: October 2016

Di Indonesia bahasa Inggris merupakan bahasa asing untuk dipelajari. Dalam prakteknya baik guru dan siswa masih menghadapi banyak masalah ketika proses pembelajaran. Berbagai respon dapat ditemui di kelas terkait masalah-masalah tersebut, khususnya pada sikap siwa selama mengikuti proses pembelajaran, hasil belajar siswa, dan partisipasi dalam melaksanakan kegiatan kelompok. Penelitian ini bertujuan untuk memaparkan kesulitan-kesulitan yang dihadapi siswa dalam belajar bahasa Inggris dan faktor penyebabnya. Penelitian ini menggunakan pendekatan kualitatif dengan instrumen angket, observasi, danrekaman video. Analisis data penelitian dilakukan melalui tiga cara yaitu reduksi data, penyajian data, penarikan kesimpulan dan verifikasi. Hasil penelitian menunjukkan bahwa subjek penelitian mengalami kesulitan belajar bahasa Inggris yang beragam. Hal tersebut terjadi akibat factor tingkat penguasaan bahasa Inggris yang berbeda-beda.
Peningkatan Kemampuan Listening Comprehension Melalui Stategi Top-Down dan Bottom-Up
Authors: Mandarani Vidya
Published: October 2016

Pemahaman listening masih menjadi masalah besar bagi pebelajar Bahasa Inggris, sehingga strategi Top-Down dan Bottom-Up merupakan salah satu strategi yang dapat membantu dalam memahami dan mengerti Listening Comprehension. Top-Down processing mengacu pada penggunaan skemata atau pengetahuan pebelajar untuk memahami informasi yang diterima, sedangkan Bottom-Up processing mengacu pada proses pemahaman informasi melalui analisis bunyi, arti kata, maupun tata bahasa. Dalam penerapannya, strategi Top-Down dan Bottom-Up di dalam kegiatan Pre-listening, While-listening dan Post-listening. Di dalam kegiatan Pre-listening kegiatan yang dibangun adalah pebelajar dapat menghubungkan konteks dari apa yang didengar dengan latar belakang pengetahuan yang dimiliki. Di dalam kegiatan While-listening, pebelajar memprediksi apa yang penting dan yang tidak penting untuk diperhatikan untuk mendorong siswa memahami apa yang mereka dengar. Di dalam kegiatan post-listening, pebelajar didorong untuk menanggapi apa yang mereka dengar. Keseluruhan kegiatan pembelajaran diharapkan dapat meningkatkan kemampuan dalam Listening Comprehension di kelas.
Pengaruh Pemahaman Konsep Matematika Vektor Mahasiswa FMIPA UNIPDU Terhadap Kemampuan Pemecahan Masalah Fisika Mekanika
Authors: Wardani Indra Kusuma
Published: October 2016

Penelitian ini merupakan penelitian tindakan kelas yang bertujuan untuk mengetahui pengaruh pemahaman konsep vektor dari subjek terhadap kemampuan pemecahan masalah fisika mekanika. Penelitian dilaksanakan dalam dua siklus yang masing-masing siklus terdiri dari empat tahap penelitian, yaitu perencanaan, pelaksanaan, pengamatan, dan refleksi. Data penelitian dianalisis berdasarkan nilai n-gain dari pre-test dan post-test pada setiap siklus penelitian. Hasil penelitian menunjukkan peningkatan n-gain berkriteria tinggi pada siklus II sebesar 0,70. Kemampuan pemecahan masalah dominan pada indikator pengajuan argumentasi dengan nilai n-gain sebesar 0,73. Keterlaksanaan rencana kegiatan pembelajaran pada setiap siklus menunjukkan nilai reliabilitas di atas 80% dengan aktivitas mahasiswa dominan pada kegiatan mengerjakan LKM. Berdasarkan temuan penelitian ini disimpulkan bahwa pemahaman matematika vektor memberikan pengaruh positif terhadap kemampuan pemecahan masalah fisika mekanika.