bluuebunny/crossref_metadata_2025 huggingface dataset:
MiR-200c-3p Modulates Cisplatin Resistance in Biliary Tract Cancer by ZEB1-Independent Mechanisms
Authors: Posch Florian , Prinz Felix , Balihodzic Amar , Mayr Christian , Kiesslich Tobias , Klec Christiane , Jonas Katharina , Barth Dominik A. , Riedl Jakob M. , Gerger Armin , Pichler Martin
Published: August 2021

Biliary tract cancer is a major global health issue in cancer-related mortality. Therapeutic options are limited, and cisplatin-based treatment schedules represent the mainstay of first-line therapeutic strategies. Although the gain of survival by the addition of cisplatin to gemcitabine is moderate, acquired cisplatin resistance frequently leads to treatment failures with mechanisms that are still poorly understood. Epithelial–mesenchymal transition (EMT) is a dynamic process that changes the shape, function, and gene expression pattern of biliary tract cancer cells. In this study, we explored the influence of the EMT-regulating miR-200c-3p on cisplatin sensitivity in biliary tract cancer cells. Using gain of function experiments, we demonstrated that miR-200c-3p regulates epithelial cell markers through the downregulation of the transcription factor ZEB1. MiR-200c-3p upregulation led to a decreased sensitivity against cisplatin, as observed in transient overexpression models as well as in cell lines stably overexpressing miR-200c-3p. The underlying mechanism seems to be independent of miR-200c-3p’s influence on ZEB1 expression, as ZEB1 knockdown resulted in the opposite effect on cisplatin resistance, which was abolished when ZEB1 knockdown and miR-200c-3p overexpression occurred in parallel. Using a gene panel of 40 genes that were previously associated with cisplatin resistance, two (Dual Specificity Phosphatase 16 (DUSP16) and Stratifin (SFN)) were identified as significantly (>2 fold, p-value < 0.05) up-regulated in miR-200c-3p overexpressing cells. In conclusion, miR-200c-3p might be an important contributor to cisplatin resistance in biliary tract cancer, independently of its interaction with ZEB1.
Application of the 21-Gene Recurrence Score in Patients with Early HR-Positive/HER2-Negative Breast Cancer: Chemotherapy and Survival Rate According to Clinical Risk
Authors: Bae Soong June , Ahn Sung Gwe , Ji Jung Hwan , Chu Chihhao , Kim Dooreh , Lee Janghee , Cha Yoon Jin , Jeong Joon
Published: August 2021

We assessed the impact of 21-gene Recurrence Score (RS) assay on chemotherapy decision-making according to binary clinical risk stratification in patients with hormone receptor (HR)-positive/human epidermal growth factor receptor 2 (HER2)-negative early breast cancer. We included patients with tumors measuring 1–5 cm, N0-1, and HR+/HER2- breast cancer who underwent surgery followed by adjuvant treatment. The clinical risk was determined by a modified version of Adjuvant! Online. We performed propensity score matching (PSM) according to the application of 21-gene RS separately in the low and high clinical risk groups. Before PSM, 342 (39.0%) of 878 patients were classified as having high clinical risk. In the high clinical risk group, 21-gene RS showed a significantly reduced chemotherapy rate of 39.3%, without increasing the recurrence. After PSM, the 21-gene RS application significantly reduced chemotherapy rate by 34.0% in 200 patients with high clinical risk (21-gene RS application, 32.0% vs. no 21-gene RS application, 66.0%, p < 0.001). There was also no significant difference in RFS according to 21-gene RS status in the high clinical risk group (log-rank test, p = 0.467). These results support the usefulness of the 21-gene RS to reduce the chemotherapy rate without adversely affecting prognosis in a high clinical risk group.
Overexpression of Reticulon 3 Enhances CNS Axon Regeneration and Functional Recovery after Traumatic Injury
Authors: Alhajlah Sharif , Thompson Adam M , Ahmed Zubair
Published: August 2021

CNS neurons are generally incapable of regenerating their axons after injury due to several intrinsic and extrinsic factors, including the presence of axon growth inhibitory molecules. One such potent inhibitor of CNS axon regeneration is Reticulon (RTN) 4 or Nogo-A. Here, we focused on RTN3 as its contribution to CNS axon regeneration is currently unknown. We found that RTN3 expression correlated with an axon regenerative phenotype in dorsal root ganglion neurons (DRGN) after injury to the dorsal columns, a well-characterised model of spinal cord injury. Overexpression of RTN3 promoted disinhibited DRGN neurite outgrowth in vitro and dorsal column axon regeneration/sprouting and electrophysiological, sensory and locomotor functional recovery after injury in vivo. Knockdown of protrudin, however, ablated RTN3-enhanced neurite outgrowth/axon regeneration in vitro and in vivo. Moreover, overexpression of RTN3 in a second model of CNS injury, the optic nerve crush injury model, enhanced retinal ganglion cell (RGC) survival, disinhibited neurite outgrowth in vitro and survival and axon regeneration in vivo, an effect that was also dependent on protrudin. These results demonstrate that RTN3 enhances neurite outgrowth/axon regeneration in a protrudin-dependent manner after both spinal cord and optic nerve injury.
The Role of microRNAs in NK Cell Development and Function
Authors: Nanbakhsh Arash , Malarkannan Subramaniam
Published: August 2021

The clinical use of natural killer (NK) cells is at the forefront of cellular therapy. NK cells possess exceptional antitumor cytotoxic potentials and can generate significant levels of proinflammatory cytokines. Multiple genetic manipulations are being tested to augment the anti-tumor functions of NK cells. One such method involves identifying and altering microRNAs (miRNAs) that play essential roles in the development and effector functions of NK cells. Unique miRNAs can bind and inactivate mRNAs that code for cytotoxic proteins. MicroRNAs, such as the members of the Mirc11 cistron, downmodulate ubiquitin ligases that are central to the activation of the obligatory transcription factors responsible for the production of inflammatory cytokines. These studies reveal potential opportunities to post-translationally enhance the effector functions of human NK cells while reducing unwanted outcomes. Here, we summarize the recent advances made on miRNAs in murine and human NK cells and their relevance to NK cell development and functions.
Calpain-Mediated Mitochondrial Damage: An Emerging Mechanism Contributing to Cardiac Disease
Authors: Zhang Mengxiao , Wang Grace , Peng Tianqing
Published: August 2021

Calpains belong to the family of calcium-dependent cysteine proteases expressed ubiquitously in mammals and many other organisms. Activation of calpain is observed in diseased hearts and is implicated in cardiac cell death, hypertrophy, fibrosis, and inflammation. However, the underlying mechanisms remain incompletely understood. Recent studies have revealed that calpains target and impair mitochondria in cardiac disease. The objective of this review is to discuss the role of calpains in mediating mitochondrial damage and the underlying mechanisms, and to evaluate whether targeted inhibition of mitochondrial calpain is a potential strategy in treating cardiac disease. We expect to describe the wealth of new evidence surrounding calpain-mediated mitochondrial damage to facilitate future mechanistic studies and therapy development for cardiac disease.
Proteomic-Based Analysis of Hypoxia- and Physioxia-Responsive Proteins and Pathways in Diffuse Large B-Cell Lymphoma
Authors: Duś-Szachniewicz Kamila , Gdesz-Birula Katarzyna , Zduniak Krzysztof , Wiśniewski Jacek R.
Published: August 2021

Hypoxia is a common feature in most tumors, including hematological malignancies. There is a lack of studies on hypoxia- and physioxia-induced global proteome changes in lymphoma. Here, we sought to explore how the proteome of diffuse large B-cell lymphoma (DLBCL) changes when cells are exposed to acute hypoxic stress (1% of O2) and physioxia (5% of O2) for a long-time. A total of 8239 proteins were identified by LC–MS/MS, of which 718, 513, and 486 had significant changes, in abundance, in the Ri-1, U2904, and U2932 cell lines, respectively. We observed that changes in B-NHL proteome profiles induced by hypoxia and physioxia were quantitatively similar in each cell line; however, differentially abundant proteins (DAPs) were specific to a certain cell line. A significant downregulation of several ribosome proteins indicated a translational inhibition of new ribosome protein synthesis in hypoxia, what was confirmed in a pathway enrichment analysis. In addition, downregulated proteins highlighted the altered cell cycle, metabolism, and interferon signaling. As expected, the enrichment of upregulated proteins revealed terms related to metabolism, HIF1 signaling, and response to oxidative stress. In accordance to our results, physioxia induced weaker changes in the protein abundance when compared to those induced by hypoxia. Our data provide new evidence for understanding mechanisms by which DLBCL cells respond to a variable oxygen level. Furthermore, this study reveals multiple hypoxia-responsive proteins showing an altered abundance in hypoxic and physioxic DLBCL. It remains to be investigated whether changes in the proteomes of DLBCL under normoxia and physioxia have functional consequences on lymphoma development and progression.
Platelet Bone Morphogenetic Protein-4 Mediates Vascular Inflammation and Neointima Formation after Arterial Injury
Authors: Jank Marietta , von Niessen Nikolaus , Olivier Christoph B. , Schmitt Hannah , Anto-Michel Nathaly , Hilgendorf Ingo , Bode Christoph , Moser Martin , Esser Jennifer S. , Zhou Qian
Published: August 2021

The purpose of this study is to investigate the role of platelet bone morphogenetic proteins (BMP)-4 during vascular inflammation and remodeling in a mouse model of carotid wire injury. Transgenic mice with a platelet-specific deletion of BMP-4 (BMP4Plt−/−) were generated. Intravital microscopy was performed to evaluate leukocyte adhesion to the vessel wall. Expression of adhesion molecules and chemokines were analyzed. Platelet-leukocyte aggregates (PLAs) were evaluated using flow cytometry. For carotid wire injury, BMP4Plt−/− mice were further crossed with LDLr−/− mice (BMP4Plt−/−/LDLr−/−) and fed with a high cholesterol diet for 2-weeks. Carotid wire injury was performed, and re-endothelialization and neointimal formation were evaluated. In comparison to the control mice, stimulation with TNFα resulted in fewer rolling and adherent leukocytes to the vessel wall in the BMP4Plt−/− mice. mRNA and protein expression of P-selectin and adhesion molecules were reduced in the aorta of the BMP4Plt−/− mice. In platelets from the BMP4Plt−/− mice, the expression of P-selectin was reduced, and fewer PLA formations were measured than in the control mice. Loss of platelet BMP-4 further prevented neointima formation after carotid wire injury. Endothelial regeneration after injury was decelerated in the BMP4Plt−/− mice, and confirmed in-vitro, where the deletion of platelet BMP-4 inhibited endothelial cell proliferation and migration. We demonstrate for the first time that platelet BMP-4 is involved during vascular inflammation and remodeling. This is partially mediated by the inhibition of platelet activation, reduced expression of adhesion molecules and inflammatory responses. Our findings identify platelet BMP-4 as a mediator of vascular inflammation in early atherosclerosis and restenosis.
Kynurenine Monooxygenase Expression and Activity in Human Astrocytomas
Authors: Vázquez Cervantes Gustavo Ignacio , Pineda Benjamín , Ramírez Ortega Daniela , Salazar Alelí , González Esquivel Dinora Fabiola , Rembao Daniel , Zavala Vega Sergio , Gómez-Manzo Saúl , Pérez de la Cruz Gonzalo , Pérez de la Cruz Verónica
Published: August 2021

Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor. The enzyme indoleamine-2,3-dioxygenase (IDO), which participates in the rate-limiting step of tryptophan catabolism through the kynurenine pathway (KP), is associated with poor prognosis in patients with GBM. The metabolites produced after tryptophan oxidation have immunomodulatory properties that can support the immunosuppressor environment. In this study, mRNA expression, protein expression, and activity of the enzyme kynurenine monooxygenase (KMO) were analyzed in GBM cell lines (A172, LN-18, U87, U373) and patient-derived astrocytoma samples. KMO mRNA expression was assessed by real-time RT-qPCR, KMO protein expression was evaluated by flow cytometry and immunofluorescence, and KMO activity was determined by quantifying 3-hydroxykynurenine by HPLC. Heterogenous patterns of both KMO expression and activity were observed among the GBM cell lines, with the A172 cell line showing the highest KMO expression and activity. Higher KMO mRNA expression was observed in glioma samples than in patients diagnosed with only a neurological disease; high KMO mRNA expression was also observed when using samples from patients with GBM in the TCGA program. The KMO protein expression was localized in GFAP+ cells in tumor tissue. These results suggest that KMO is a relevant target to be explored in glioma since it might play a role in supporting tumor metabolism and immune suppression.
The Role of the Hedgehog Pathway in Chemoresistance of Gastrointestinal Cancers
Authors: Liang Yabing , Yang Ling , Xie Jingwu
Published: August 2021

The hedgehog pathway, which plays a significant role in embryonic development and stem cell regulation, is activated in gastrointestinal cancers. Chemotherapy is widely used in cancer treatment. However, chemoresistance becomes a substantial obstacle in cancer therapy. This review focuses on the recent advances in the hedgehog pathway’s roles in drug resistance of gastrointestinal cancers and the novel drugs and strategies targeting hedgehog signaling.
Immune Microenvironment Landscape in CNS Tumors and Role in Responses to Immunotherapy
Authors: Najem Hinda , Khasraw Mustafa , Heimberger Amy B.
Published: August 2021

Despite the important evolution of immunotherapeutic agents, brain tumors remain, in general, refractory to immune therapeutics. Recent discoveries have revealed that the glioma microenvironment includes a wide variety of immune cells in various states that play an important role in the process of tumorigenesis. Anti-tumor immune activity may be occurring or induced in immunogenic hot spots or at the invasive edge of central nervous system (CNS) tumors. Understanding the complex heterogeneity of the immune microenvironment in gliomas will likely be the key to unlocking the full potential of immunotherapeutic strategies. An essential consideration will be the induction of immunological effector responses in the setting of the numerous aspects of immunosuppression and evasion. As such, immune therapeutic combinations are a fundamental objective for clinical studies in gliomas. Through immune profiling conducted on immune competent murine models of glioma and ex vivo human glioma tissue, we will discuss how the frequency, distribution of immune cells within the microenvironment, and immune modulatory processes, may be therapeutically modulated to lead to clinical benefits.
Improving In Vitro Culture of Human Male Fetal Germ Cells
Authors: Martin-Inaraja Myriam , Ferreira Monica , Taelman Jasin , Eguizabal Cristina , Chuva De Sousa Lopes Susana M.
Published: August 2021

Male human fetal germ cells (hFGCs) give rise to spermatogonial stem cells (SSCs), which are the adult precursors of the male gametes. Human SSCs are a promising (autologous) source of cells for male fertility preservation; however, in contrast to mouse SSCs, we are still unable to culture them in the long term. Here, we investigated the effect of two different culture media and four substrates (laminin, gelatin, vitronectin and matrigel) in the culture of dissociated second trimester testes, enriched for hFGCs. After 6 days in culture, we quantified the presence of POU5F1 and DDX4 expressing hFGCs. We observed a pronounced difference in hFGC number in different substrates. The combination of gelatin-coated substrate and medium containing GDNF, LIF, FGF2 and EGF resulted in the highest percentage of hFGCs (10% of the total gonadal cells) after 6 days of culture. However, the vitronectin-coated substrate resulted in a comparable percentage of hFGCs regardless of the media used (3.3% of total cells in Zhou-medium and 4.8% of total cells in Shinohara-medium). We provide evidence that not only the choices of culture medium but also choices of the adequate substrate are crucial for optimizing culture protocols for male hFGCs. Optimizing culture conditions in order to improve the expansion of hFGCs will benefit the development of gametogenesis assays in vitro.
Interaction between Autonomic Regulation, Adiposity Indexes and Metabolic Profile in Children and Adolescents with Overweight and Obesity
Authors: Calcaterra Valeria , Palombo Carlo , Malacarne Mara , Pagani Massimo , Federico Giovanni , Kozakova Michaela , Zuccotti Gianvincenzo , Lucini Daniela
Published: August 2021

Early obesity predicts initial modifications in cardiac and vascular autonomic regulation. The aim of this study was to assess the possible interaction between non-invasive measures of autonomic cardiovascular control and peripheral endothelium regulation in children with overweight and obesity. We involved 114 young subjects (77M/37F, 12.7 ± 2.2 years) with normal weight (NW, n = 46) to overweight or obesity (OB, n = 68). Multivariate statistical techniques utilizing a collection of modern indices of autonomic regulation, adiposity indexes and metabolic profile were employed. Resting values show substantial equivalence of data. Conversely, blood pressure variance is greater in NW/OB groups. The correlation matrix between major autonomic and metabolic/hemodynamic variables shows a clustered significant correlation between homogeneous indices. A significant correlation between metabolic indices and endothelial and autonomic control, mostly in its vascular end, was recorded. Particularly, the alpha index is significantly correlated with triglycerides (r = −0.261) and endothelial indices (RHI, r = 0.276). Children with obesity show a link between indices of autonomic and endothelial function, fat distribution and metabolic profile. The optimization of autonomic control, for instance by exercise/nutrition interventions, could potentially prevent/delay the occurrence of structural vascular damage leading to reduced cardiovascular health.
Differential Diagnosis of Acquired and Hereditary Neuropathies in Children and Adolescents—Consensus-Based Practice Guidelines
Authors: Korinthenberg Rudolf , Trollmann Regina , Plecko Barbara , Stettner Georg M. , Blankenburg Markus , Weis Joachim , Schoser Benedikt , Müller-Felber Wolfgang , Lochbuehler Nina , Hahn Gabriele , Rudnik-Schöneborn Sabine
Published: August 2021

Disorders of the peripheral nerves can be caused by a broad spectrum of acquired or hereditary aetiologies. The objective of these practice guidelines is to provide the reader with information about the differential diagnostic workup for a target-oriented diagnosis. Following an initiative of the German-speaking Society of Neuropaediatrics, delegates from 10 German societies dedicated to neuroscience worked in close co-operation to write this guideline. Applying the Delphi methodology, the authors carried out a formal consensus process to develop practice recommendations. These covered the important diagnostic steps both for acquired neuropathies (traumatic, infectious, inflammatory) and the spectrum of hereditary Charcot–Marie–Tooth (CMT) diseases. Some of our most important recommendations are that: (i) The indication for further diagnostics must be based on the patient’s history and clinical findings; (ii) Potential toxic neuropathy also has to be considered; (iii) For focal and regional neuropathies of unknown aetiology, nerve sonography and MRI should be performed; and (iv) For demyelinated hereditary neuropathy, genetic diagnostics should first address PMP22 gene deletion: once that has been excluded, massive parallel sequencing including an analysis of relevant CMT-genes should be performed. This article contains a short version of the guidelines. The full-length text (in German) can be found at the Website of the “Arbeitsgemeinschaft der Wissenschftlichen Medizinischen Fachgesellschaften e.V. (AWMF), Germany.
Fiber Reinforced Polymer as Wood Roof-to-Wall Connections to Withstand Hurricane Wind Loads
Authors: Dhakal Aman , Parvin Azadeh
Published: August 2021

Light wood roof-to-wall connections are vulnerable when subjected to high-speed winds. In lieu of traditional metal connections, the present finite element analysis (FEA) study focuses on the use of epoxy and easy-to-apply, noncorrosive FRP ties to connect the roof and the walls in wood frames. The FEA models of the wood roof-to-wall GFRP connection were validated with an experimental study in the literature. Subsequently parametric study was performed on the validated FEA models. Parameters considered were the addition of anchorages to secure the GFRP ties for FEA models of shear and uplift tests, and various FRP types. Wood roof-to-wall connection uplift model was subjected to monotonic cyclic loading to simulate the effect of wind load. In addition, carbon and basalt FRP ties were also examined under monotonic cyclic loading. To evaluate the efficiency of GFRP ties with and without anchorages, the shear and uplift design loads specified in ASCE 7-16 were calculated. Finally, a formula was proposed to approximate the shear strength of GFRP connection in comparison with double shear bolted metal plate connections. The FEA models and experimental results were in good agreement. The finite element results revealed that anchorage increased the uplift load capacity by 15% but the increase in shear capacity was insignificant. Comparing glass, carbon, and basalt FRP ties, BFRP was superior in deformation capacity and CFRP provided more stiffness on uplift test simulation. GFRP ties were found to be approximately nine times stronger in shear and two times stronger in uplift resistance than hurricane clips. Finally, the proposed formula could predict the shear strength of GFRP tie connection which in turns contributes to the design and future research.
Optical Losses of Frontal Layers in Superstrate CdS/CdTe Solar Cells Using OPAL2
Authors: Amin Nowshad , Karim Mohammad Rezaul , ALOthman Zeid Abdullah
Published: August 2021

In this paper, optical losses in CdS/CdTe solar cells are calculated on the basis of the designated reflective index of various frontal layers using an OPAL2 calculator for the first time. Two types of glass (0.1 mm ultra-thin Schott and 1.1 mm standard borosilicate glass) were assumed to be coated by different Transparent-Conducting-Oxides (TCOs) such as SnO2:F, ZnO:Al, and ITO forming frontal layers for CdS/CdTe solar cells in superstrate configuration. Absorption, reflectance, transmittance, and consequently optical bandgap energies are calculated as a function of common thicknesses, used in the literature. The results show that an increase in TCO thickness led to a decrease in optical band gap as well as an enhancement in contact potential difference, which can deteriorate device performance. The optimum thickness of 100 nm for SnO2:F was calculated, while 200 nm for ZnO:Al and ITO show reasonable optical losses caused by reflections at the interfaces’ and the layer’s absorption. It is seen that 80 to 150 nm CdS on ITO might be an effective range to satisfy a high short circuit current and low defect densities at the CdS/CdTe interface. Finally, a minimum 2 μm thickness for the CdTe on the ultra-thin Schott glass coated by optimum layers can result in the highest short circuit current of 28.69 mA/cm2. This work offers a practical equivalent strategy to be applied for any superstrate solar cells containing TCO and CdS frontal layers.
Structural Evaluation of Coal-Tar-Pitch-Based Carbon Materials and Their Na+ Storage Properties
Authors: Yin Ruilin , Wang Kun , Han Beibei , Xu Guiying , Li Lixiang , An Baigang , Ju Dongying , Chai Maorong , Li Songnan , Zhou Weimin
Published: August 2021

Linking to the S element hybrid strategies, S-doped carbon materials having different macrostructures and defect concentrations are prepared by using sulfur and coal-tar-pitch as raw materials in a carbonization temperature range of 700–1000 °C. The evaluations of macrostructure and surface characteristics are performed through XRD, TEM, Raman and XPS measurements. Through the linear fitting among the Na+ storage capacity with ID/IG and d002 values, the correlations of Na+ storage capacity with macrostructures and defects are respectively investigated in detail. It is observed that S-doped carbon materials exhibit storage capacity at 120 mAh/g after the charge-discharge is being carried out 2000 cycles at 2.0 A/g. Studies have shown that adsorptions of introduced defects on graphene-like carbon sheets mainly play the role to enhance the storage capacity, and the expanded carbonaceous lamellar spaces of highly disordered and pseudo-graphitic macrostructures provide the channels for fast transfer of Na+. Our studies are able to provide references for designs and fabrications of coal tar pitch based soft carbon materials as sodium-ion batteries (SIBs) anodes when using heteroatoms doping methods.
Variable Gap Sealing Technology of a Hydraulic Cylinder Based on Magnetic Shape Memory Alloy
Authors: Chen Xiaolan , Tu Fuquan , Gao Feng , Cheng Heming , Xing Shixiong
Published: August 2021

The synergistic control of resistance reduction and sealing poses challenges to enhancing the rapid dynamic response ability of servo hydraulic cylinders; the key to solving this problem is effectively controlling the sealing gap value. In this study, a micro-variation between the hydraulic cylinder and the piston based on the disadvantage of conventional seals, constant gap seals, and lip gap seals was constructed; MSMA assist support blocks were designed on the piston to form a gap seal strip; then, the sealing gap value could be changed by controlling the magnetic field intensity. Simultaneously, the effects of magnetic field strength, parts-manufacturing precision, temperature, and hysteresis on the micro-variation in the MSMA were analyzed, and effective solutions were proposed. Finally, experiments on the magnetic field, temperature, and hysteresis were conducted by the measurement system. The results showed that the variable value of the sealing gap with the MSMA is feasible under ideal conditions, and can effectively change the amount of MSMA expansion by controlling the magnetic field strength, temperature, preload, etc., and then change the amount of the sealing gap of the hydraulic cylinder. This is the key to achieving friction and sealing control, which plays a crucial and active role in improving the efficiency of hydraulic systems. However, the impact of hysteresis effects cannot be ignored, which will be the main problem to be solved in the future.
Crystal Growth on Cenospheres from High-Calcium Fly Ash
Authors: Yoriya Sorachon , Tepsri Phattarathicha
Published: August 2021

This work presents a study of cenosphere separation from lignite high-calcium (~24 wt.%) fly ash by centrifugal method; this is the first report for Mae Moh, Thailand, fly ash with this high calcium content using this technique. The effect of centrifugal parameters on cenosphere yield and properties were investigated. Those properties include physical properties, morphology, chemical composition, and mineral phases. The recovery yields are in the range of 0.34–0.64%, approximately one third of the yield obtained from the general gravity settling method. Density, particle size, and morphology of the collected cenospheres appeared to be independent of sequence of the applied speeds and times. Interrelation of chemical composition and mineral phases was established, with the focus on calcium carbonate formation on cenosphere surface and crystallite size study. The study has revealed the preferential formation of calcite–(104) peak is observed–by cenospheres, with stable growth behavior of crystallite sizes obtained from all the centrifugal conditions. The result was compared to that obtained from the sink-float method for a better insight. The influence and limitation of the centrifugal method, the varied parameters, and the relevant reaction pathways on crystal growth process in terms of important dissolving species (i.e., Ca2+ and CO32−) behavior in the ash suspension were discussed.
Investigation on the Effects of Grain Boundary on Deformation Behavior of Bicrystalline Pillar by Crystal Plasticity Finite Element Method
Authors: Zhou Hui , Wang Pei , Lu Shanping
Published: August 2021

A dislocation density–grain boundary interaction scheme coupled with the dislocation density-based crystalline plasticity finite element method has been established and used to investigate the deformation behavior of bicrystalline pillars with the same grain boundary misorientation angle but different crystal orientations. It is found that the angle between the activated slip systems, which is determined by the crystal orientations, rather than the grain boundary misorientation angle, influences the interactions between the plastic slip and the grain boundary, which further influence the heterogeneous deformation of bicrystalline specimens.
Heterogeneous Crystal Nucleation from the Melt in Polyethylene Oxide Droplets on Graphite: Kinetics and Microscopic Structure
Authors: Tariq Muhammad , Thurn-Albrecht Thomas , Dolynchuk Oleksandr
Published: August 2021

It is well known that the crystallization of liquids often initiates at interfaces to foreign solid surfaces. In this study, using polarized light optical microscopy, atomic force microscopy (AFM), and wide-angle X-ray scattering (WAXS), we investigate the effect of substrate–material interactions on nucleation in an ensemble of polyethylene oxide (PEO) droplets on graphite and on amorphous polystyrene (PS). The optical microscopy measurements during cooling with a constant rate explicitly evidenced that the graphite substrate enhances the nucleation kinetics, as crystallization occurred at approximately an 11 °C higher temperature than on PS due to changes in the interactions at the solid interface. This observation allowed us to conclude that graphite induces heterogeneous nucleation in PEO. By employing the classical nucleation theory for analysis of the data with reference to the amorphous PS substrate, the obtained results indicated that the crystal nuclei with contact angles in the range of 100–117° were formed at the graphite interface. Furthermore, we show that heterogeneous nucleation led to a preferred orientation of PEO crystals on graphite, whereas PEO crystals on PS had isotropic orientation. The difference in crystal orientations on the two substrates was also confirmed with AFM, which showed only edge-on lamellae in PEO droplets on graphite compared to unoriented lamellae on PS.
VHR-REA_IT Dataset: Very High Resolution Dynamical Downscaling of ERA5 Reanalysis over Italy by COSMO-CLM
Authors: Raffa Mario , Reder Alfredo , Marras Gian Franco , Mancini Marco , Scipione Gabriella , Santini Monia , Mercogliano Paola
Published: August 2021

This work presents a new dataset for recent climate developed within the Highlander project by dynamically downscaling ERA5 reanalysis, originally available at ≃31 km horizontal resolution, to ≃2.2 km resolution (i.e., convection permitting scale). Dynamical downscaling was conducted through the COSMO Regional Climate Model (RCM). The temporal resolution of output is hourly (like for ERA5). Runs cover the whole Italian territory (and neighboring areas according to the necessary computation boundary) to provide a very detailed (in terms of space–time resolution) and comprehensive (in terms of meteorological fields) dataset of climatological data for at least the last 30 years (01/1989-12/2020). These types of datasets can be used for (applied) research and downstream services (e.g., for decision support systems).
The Influence of the Characteristics of the Medium Voltage Network on the Single Line-to-Ground Fault Current in the Resistor Grounded Neutral Networks
Authors: Toader Dumitru , Greconici Marian , Vesa Daniela , Vintan Maria , Solea Claudiu , Maghet Adrian , Tatai Ildiko
Published: August 2021

One important problem in the operation of medium voltage networks is the detection of a single-line-to-ground fault in its incipient state, when the fault resistance values are very high. In a medium voltage (MV) distribution network with a neutral grounding resistor (NGR), one of the methods employed to discriminate a single line-to-ground fault is the use of an overcurrent relay with an operating characteristic adjusted according to the effective value of the current flowing through the limiting resistor. In case of a single line-to-ground fault with a high fault resistance value, the correct tripping settings of the protective relay require the precise computation of this current. In comparison to the assumptions made by the models from the literature—the three-phase voltage system of the medium voltage busbars is symmetrical and there are no active power losses in the network insulation—the model proposed in this paper considers the pre-fault zero-sequence voltage of the medium voltage busbars and the active power losses in the network insulation, which is necessary in certain fault conditions where the use of the former leads to unacceptable errors.
Myocardial Postsystolic Shortening and Early Systolic Lengthening: Current Status and Future Directions
Authors: Brainin Philip
Published: August 2021

The concept of paradoxical myocardial deformation, commonly referred to as postsystolic shortening and early systolic lengthening, was originally described in the 1970s when assessed by invasive cardiac methods, such as ventriculograms, in patients with ischemia and animal experimental models. Today, novel tissue-based imaging technology has revealed that these phenomena occur far more frequently than first described. This article defines these deformational patterns, summarizes current knowledge about their existence and highlights the clinical potential associated with their understanding.
Effect of Liraglutide on Vascular Inflammation Evaluated by [64Cu]DOTATATE
Authors: Zobel Emilie H. , Ripa Rasmus S. , von Scholten Bernt J. , Curovic Viktor Rotbain , Diaz Lars Jorge , Hansen Tine W. , Rossing Peter , Kjaer Andreas
Published: August 2021

Quantification of vascular inflammation before and after treatment with glucagon-like peptide-1 receptor agonists (GLP-1 RAs) may help reveal mechanistic pathways underlying the cardiovascular benefits of these drugs. We assessed change in vascular inflammation in the carotid arteries over 26 weeks by copper-64-labeled [1,4,7,10-tetraazacyclododecane-N,N′,N″,N‴-tetraacetic acid]-D-Phe1, Tyr3-octreotate ([64Cu]DOTATATE) PET in 30 participants included in a substudy of a double-blind trial where persons with type 2 diabetes (T2D) were randomized to liraglutide (n = 15) or placebo (n = 15) for 26 weeks. Mean age (SD) was 66.4 (7.2) years, HbA1c 56.4 (9.2) mmol/mol and BMI 28.9 (4.6) kg/m2. Weight and HbA1c were significantly reduced by liraglutide vs. placebo (p ≤ 0.01). The [64Cu]DOTATATE uptake (mean standardized uptake values) was significantly reduced in the liraglutide-treated group (−0.11 [95% confidence interval −0.19 to −0.03], p = 0.01) and not changed significantly in the placebo group (−0.07 [−0.14 to 0.01], p = 0.08). The mean difference between groups did not reach significance (−0.04 [−0.15 to 0.07], p = 0.44). In conclusion, [64Cu]DOTATATE uptake was reduced in persons with T2D treated with liraglutide. However, the reduction compared to placebo did not reach statistical significance, perhaps due to limited power. A reduction in vascular inflammation with liraglutide could help explain the cardiovascular protection observed with GLP-1 RAs in outcome studies but warrants further and larger studies.
Individual Tree Crown Delineation for the Species Classification and Assessment of Vital Status of Forest Stands from UAV Images
Authors: Safonova Anastasiia , Hamad Yousif , Dmitriev Egor , Georgiev Georgi , Trenkin Vladislav , Georgieva Margarita , Dimitrov Stelian , Iliev Martin
Published: August 2021

Monitoring the structure parameters and damage to trees plays an important role in forest management. Remote-sensing data collected by an unmanned aerial vehicle (UAV) provides valuable resources to improve the efficiency of decision making. In this work, we propose an approach to enhance algorithms for species classification and assessment of the vital status of forest stands by using automated individual tree crowns delineation (ITCD). The approach can be potentially used for inventory and identifying the health status of trees in regional-scale forest areas. The proposed ITCD algorithm goes through three stages: preprocessing (contrast enhancement), crown segmentation based on wavelet transformation and morphological operations, and boundaries detection. The performance of the ITCD algorithm was demonstrated for different test plots containing homogeneous and complex structured forest stands. For typical scenes, the crown contouring accuracy is about 95%. The pixel-by-pixel classification is based on the ensemble supervised classification method error correcting output codes with the Gaussian kernel support vector machine chosen as a binary learner. We demonstrated that pixel-by-pixel species classification of multi-spectral images can be performed with a total error of about 1%, which is significantly less than by processing RGB images. The advantage of the proposed approach lies in the combined processing of multispectral and RGB photo images.
Reconstructing the Changes in Sedimentation and Source Provenance in East African Hydropower Reservoirs: A Case Study of Nyumba ya Mungu in Tanzania
Authors: Amasi Aloyce I. M. , Wynants Maarten , Kawalla Remegius A. , Sawe Shovi , Munishi Linus , Blake William H. , Mtei Kelvin M.
Published: August 2021

This study aimed to reconstruct the sedimentation rates over time and identify the changing sources of sediment in a major hydropower reservoir in Tanzania, the Nyumba ya Mungu (NYM). Fallout 210Pb measurements were used to estimate age of sediment deposits and broad changes in sedimentation rates were reconstructed. Sedimentation peaks were cross referenced to geochemical profiles of allogenic and autogenic elemental constituents of the sediment column to confirm a causal link. Finally, geochemical fingerprinting of the sediment cores and potential sources were compared using a Bayesian mixing model (MixSIAR) to attribute the dominant riverine and land use sources to the reservoir together with changes through recent decades. Reservoir sedimentation generally increased from 0.1 g cm−2 yr−1 in the lower sediment column to 1.7 g cm−2 yr−1 in the most recent deposits. These results correlated to changes in allogenic and autogenic tracers. The model output pointed to one of two major tributaries, the Kikuletwa River with 60.3%, as the dominant source of sediment to the entire reservoir, while the other tributary, Ruvu River, contributed approximately 39.7%. However, downcore unmixing results indicated that the latest increases in sedimentation seem to be mainly driven by an increased contribution from the Ruvu River. Cultivated land (CU) was shown to be the main land use source of riverine sediment, accounting for 38.4% and 44.6% in Kikuletwa and Ruvu rivers respectively. This study explicitly demonstrated that the integration of sediment tracing and dating tools can be used for quantifying the dominant source of sediment infilling in East African hydropower reservoirs. The results underscore the necessity for catchment-wide management plans that target the reduction of both hillslope erosion reduction and the sediment connectivity from hillslope source areas to rivers and reservoirs, which will help to maintain and enhance food, water and energy security in Eastern Africa.
Educating Informal Educators on Issues of Race and Inequality: Raising Critical Consciousness, Identifying Challenges, and Implementing Change in a Youth and Community Work Programme
Authors: Achilleos Jess , Douglas Hayley , Washbrook Yasmin
Published: August 2021

The debate regarding institutional racism and White privilege within higher education (HE) remains prevalent, and higher education institutions (HEIs) are not exempt from the racial equality debate. Youth and Community Work is underpinned by anti-oppressive practice, highlighting a need to educate informal educators on the structural underpinnings of Race and inequlaity, so that they can be challenged in practice to bring about social change. For Youth and Community Workers, this is primarily done through informal education and critical pedagogy. The research aimed to unearth race inequality within the Youth and Community Work programme at Wrexham Glyndŵr University (WGU). Critical reflection methodology was used to deconstruct departmental processes of recruitment, learning and assessment, student voice, and support. Research data was analysed using thematic analysis, determining three themes: critical consciousness, challenge, and change. These are discussed within the framework of Critical Race Theory and critical pedagogy. The research concludes that oppression, and therefore inequality, occurs in the Youth and Community Work programme. Further reading of issues reported in HEIs across the United Kingdom shows that more analysis and deconstruction is needed through CRT. Educating informal educators on issues of race and inequality to raise critical consciousness is one way this can be achieved.
Review about Online Educational Guidance during the COVID-19 Pandemic
Authors: Mielgo-Conde Iván , Seijas-Santos Sara , Grande-de-Prado Mario
Published: August 2021

The COVID-19 pandemic has affected almost every area of our lives. It meant a break from our lifestyles, transforming everything from face-to-face to online. Among the places where this situation has become more evident are primary schools, secondary schools, and universities, which have had to adopt online teaching in its entirety. Faced with this situation, the work of the educational counsellors was essential to coordinate the centre with the families and to enable students to follow the classes in the best way possible. The objective of this study is to analyse the scientific literature about online educational guidance, with a focus on the COVID-19 pandemic. To achieve this, a scoping review inspired by the PRISMA model was done. A total of 42 articles were studied. The main results show that many counsellors were at a disadvantage and in a very stressful situation, as they did not have the necessary digital training. On the other hand, this situation has been the first contact for many counsellors with the use of ICT within their profession and in an unexpected situation, without time for training or enough preparation.
A Corruption Course through a Culturally Relevant Pedagogy: The Need for an Assessment That Fits
Authors: El Alaoui Khadija , Pilotti Maura A. E.
Published: August 2021

The present study examines the challenges and the rewards of assessing learning in a seminar on corruption which is taught in a country (Iraq) where political corruption is seen as the main source of structural instability and sectarian tensions. It specifically focuses on the role of culturally relevant pedagogy in defining the most suitable summative assessment. To this end, essay test questions were developed concerning the relevance of political corruption, the impact of investigative journalism, the role of humor in investigative journalism, and the appropriate remedies (besides journalism) to political corruption. In students’ responses, investigative journalism (with or without humor) was consistently reported to be a dangerous undertaking, but disagreement emerged on its effectiveness as one of the possible remedies for corruption. Dishonesty was seen as the universal cancer of political systems and thus difficult to extirpate, albeit some systems (e.g., Al-Muhasasa) were seen as tolerating it more than others. In our study, the qualitative examination of students’ responses served three objectives: (a) it offered evidence about the extent to which culturally relevant pedagogy was realized in the course; (b) it served to put forth a proposal on how students’ test responses can inform teaching and assessment in future offerings of the course; and more broadly, (c) it synopsized the views of a sample of college students who represent the population upon whom the country heavily relies for its economic and political recovery.
Natural Ventilation of Toilet Units in K–12 School Restrooms Using CFD
Authors: Lin Yi-Pin
Published: August 2021

In this study, the influence of architectural design parameters (the exterior window area, the wall height at a corridor-side, and the door gap of a toilet unit) on the airflow patterns and malodorous volatile substance (acetic acid) distributions within toilet units were investigated via computational fluid dynamics (CFD), with the restrooms in K–12 public schools (kindergarten through grade 12) of Taiwan as research objects. The results show that when there is a 2 m/s north wind in winter, all the cases exceed the required 12 air changes per hour (ACH), and most are above 43.75 ACH. When there is a 0.5 m/s south wind in summer, nearly half of the cases fail to reach 12 ACH. Maintaining an adequate natural ventilation rate and an acceptable level of odor pollution through passive design and architectural design is difficult. Thinking about how to improve the ventilation efficiency of toilet units with the aid of simple, appropriate, and energy-saving mechanical ventilation approaches is necessary.
Indoor and Outdoor Nanoparticle Concentrations in an Urban Background Area in Northern Sweden: The NanoOffice Study
Authors: Orru Hans , Hagenbjörk Annika , Olstrup Henrik
Published: August 2021

In recent years, nanoparticles (NPs) have received much attention due to their very small size, high penetration capacity, and high toxicity. In urban environments, combustion-formed nanoparticles (CFNPs) dominate in particle number concentrations (PNCs), and exposure to those particles constitutes a risk to human health. Even though fine particles (<2.5 µm) are regularly monitored, information on NP concentrations, both indoors and outdoors, is still limited. In the NanoOffice study, concentrations of nanoparticles (10–300 nm) were measured both indoors and outdoors with a 5-min time resolution at twelve office buildings in Umeå. Measurements were taken during a one-week period in the heating season and a one-week period in the non-heating season. The measuring equipment SMPS 3938 was used for indoor measurements, and DISCmini was used for outdoor measurements. The NP concentrations were highest in offices close to a bus terminal and lowest in offices near a park. In addition, a temporal effect appeared, usually with higher concentrations of nanoparticles found during daytime in the urban background area, whereas considerably lower nanoparticle concentrations were often present during nighttime. Infiltration of nanoparticles from the outdoor air into the indoor air was also common. However, the indoor/outdoor ratios (I/O ratios) of NPs showed large variations between buildings, seasons, and time periods, with I/O ratios in the range of 0.06 to 0.59. The reasons for high indoor infiltration rates could be NP emissions from adjacent outdoor sources. We could also see particle growth since the indoor NPs were, on average, almost twice as large as the NPs measured outdoors. Despite relatively low concentrations of NPs in the urban background air during nighttime, they could rise to very high daytime concentrations due to local sources, and those particles also infiltrated the indoor air.