bluuebunny/crossref_metadata_2025 huggingface dataset:
Response of Vegetation and Soil Characteristics to Grazing Disturbance in Mountain Meadows and Temperate Typical Steppe in the Arid Regions of Central Asian, Xinjiang
Authors: Bi Xu , Li Bo , Xu Xiangchao , Zhang Lixin
Published: June 2020

Grazing is one of the most common causes of grassland degradation, therefore, an assessment of soil physicochemical properties and plant nutrients under grazing is important for understanding its influences on ecosystem nutrient cycling and for formulating appropriate management strategies. However, the effects of grazing on grassland soil physicochemical properties and plant nutrients in mountain meadow and temperate typical steppe in the arid regions are still unclear. Therefore, we investigated the vegetation nutrient concentrations of nitrogen, phosphorus and potassium (N, P, and K) as well as soil physicochemical properties in the topmost 40 cm depth soil, to evaluate how these factors respond to grazing disturbance in a mountain meadow and temperate typical steppe within a mountain basin system in arid regions. Our results revealed that the soil bulk density values at depth of 0–40 cm increased after grazing in the mountain meadow and temperate typical steppe, whereas the soil water content decreased in the mountain meadow and increased in the temperate typical steppe after grazing. In the mountain meadow, soil total N and available P in addition to vegetation N and P concentrations increased in response to high-intensity grazing, while soil available N, available K and vegetation K decreased after grazing; in addition, soil pH, soil total P and K showed no significant changes. In the temperate typical steppe, the soil total P, soil available N, P, and K, and vegetation N, P, and K increased under relatively low-intensity grazing, whereas soil pH and soil total K showed no significant changes except for the deceasing soil total N. Our findings showed the different responses of different grassland ecosystems to grazing. Moreover, we propose that further related studies are necessary to better understand the effects of grazing on grassland ecosystems, and thereby provide a theoretical basis for the sustainable use of animal husbandry and ecological restoration of grasslands.
Prediction of the Number of Patients Infected with COVID-19 Based on Rolling Grey Verhulst Models
Authors: Zhao Yu-Feng , Shou Ming-Huan , Wang Zheng-Xin
Published: June 2020

The outbreak of a novel coronavirus (SARS-CoV-2) has caused a large number of residents in China to be infected with a highly contagious pneumonia recently. Despite active control measures taken by the Chinese government, the number of infected patients is still increasing day by day. At present, the changing trend of the epidemic is attracting the attention of everyone. Based on data from 21 January to 20 February 2020, six rolling grey Verhulst models were built using 7-, 8- and 9-day data sequences to predict the daily growth trend of the number of patients confirmed with COVID-19 infection in China. The results show that these six models consistently predict the S-shaped change characteristics of the cumulative number of confirmed patients, and the daily growth decreased day by day after 4 February. The predicted results obtained by different models are very approximate, with very high prediction accuracy. In the training stage, the maximum and minimum mean absolute percentage errors (MAPEs) are 4.74% and 1.80%, respectively; in the testing stage, the maximum and minimum MAPEs are 4.72% and 1.65%, respectively. This indicates that the predicted results show high robustness. If the number of clinically diagnosed cases in Wuhan City, Hubei Province, China, where COVID-19 was first detected, is not counted from 12 February, the cumulative number of confirmed COVID-19 cases in China will reach a maximum of 60,364–61,327 during 17–22 March; otherwise, the cumulative number of confirmed cases in China will be 78,817–79,780.
Standardization Procedure for Data Exchange
Authors: Fukami Yoshiaki
Published: June 2020

Common specification of data promotes data exchange among many and unspecified individuals and organizations. However, standardization itself tends to discourage innovation that can create new uses of data. To overcome this dilemma of innovation and standardization, this paper analyzes and proposes hypotheses regarding the process through which the World Wide Web Consortium (W3C) has realized innovations such as web applications by updating the standard. I hypothesize the following changes in standardization process management at the W3C as key factors supporting innovation through standardization among stakeholders with conflicting interests: (1) defining the scope of the specifications to be developed according to functions instead of technical structures; (2) design of a development management policy based on feedback from implementations, referred to as an “implementation-oriented policy”; (3) inclusion of diversified stakeholders in open standardization processes that facilitate consensus formation and the diffusion of developed standards; and (4) adopting a royalty-free to encourage third-party developers to implement proposed specifications and advance update of proposals. This single case analysis leads to the development and diffusion of common technological data specifications, which are the driving factors for innovation utilizing big data generated by exchanging data of various origins.
The Efficacy of Rotary, Reciprocating, and Combined Non-Surgical Endodontic Retreatment Techniques in Removing a Carrier-Based Root Canal Filling Material from Straight Root Canal Systems: A Micro-Computed Tomography Analysis
Authors: Alakabani Tarek-Fahed , Faus-Llácer Vicente , Faus-Matoses Ignacio , Ruiz-Sánchez Celia , Zubizarreta-Macho Álvaro , Sauro Salvatore , Faus-Matoses Vicente
Published: June 2020

The aim of this study is to analyze and compare the efficacy of three non-surgical endodontic retreatment techniques in removing a carrier-based root canal filling material from straight root canal systems. The study was performed on 99 single-rooted extracted teeth using the ProTaper Gold endodontic rotary system up to the F2 file (Dentsply Maillefer, Baillagues, Switzerland), which were sealed with GuttaCore (Dentsply Maillefer, Ballaigues, Switzerland) and AH plus epoxy resin sealer (Dentsply DeTrey, Konstanz, Germany) and randomly assigned to the following non-surgical retreatment techniques: ProTaper Retreatment endodontic rotary instruments (D1–D3 files, Dentsply Maillefer, Ballaigues, Switzerland; n = 33, PTR), Reciproc Blue endodontic reciprocating instrument (R50, VDW, Munich, Germany; n = 33, RCB50), and a combined root canal retreatment technique between Gates-Glidden drills (sizes #3 and #2, Dentsply Maillefer, Ballaigues, Switzerland) and Hedstrom files (file size 35, 30, and 25, Dentsply Maillefer, Ballaigues, Switzerland; n = 33; H-GG). All of the teeth were submitted twice to a micro-computed tomography (micro-CT) scan, before and after non-surgical endodontic retreatment procedures. The volume of root canal filling material (mm3), volume of remaining root canal filling material (mm3), non-surgical endodontic retreatment working time (min), proportion of remaining root canal filling material (%), and efficacy of root canal filling material removal between the non-surgical endodontic retreatment techniques were analyzed using ANOVA one-way statistical analysis. Statistically significant differences were observed between the proportions of remaining root canal filling material of PTR and H-GG (p = 0.018), between the non-surgical endodontic retreatment working times (min; p < 0.001), and between the efficacies of root canal filling material removal by the non-surgical endodontic retreatment techniques (p = 0.009). However, the non-surgical endodontic retreatment systems allow for similar carrier-based root canal filling material removal.
Outcome of Root Canal Treatments Provided by Endodontic Postgraduate Students. A Retrospective Study
Authors: Llena Carmen , Nicolescu Teodora , Perez Salvadora , Gonzalez de Pereda Silvia , Gonzalez Ana , Alarcon Iris , Monzo Angela , Sanz José Luis , Melo Maria , Forner Leopoldo
Published: June 2020

The aim of this study was to assess the preoperative, intraoperative, and postoperative factors that influenced complete periapical healing in teeth that underwent primary root canal treatment (RCT), in patients treated by postgraduate students in endodontics. Factors were retrieved and compared with the periapical status during the follow-up visit. Healing was considered as the absence of clinical and radiological symptoms. Variables significantly associated by the chi-squared test were included in a logistic regression model (LRM). Preoperative factors associated with healing were: American Society of Anesthesiology (ASA) status (p = 0.01); the absence of preoperative pain (p = 0.04); positive response to pulp tests; when the RCT cause was caries, pain, abscess, or sinus tract; probing depth <4 mm; the absence of mobility; absence or <4 mm periapical lesion (p < 0.01). In the LRM, the factors included were: absence or <4 mm periapical lesion; probing depths <4 mm; RCT caused by caries, pain, abscess, or sinus tract; the tooth was not a bridge abutment. Postoperative factors were: teeth with direct restoration; teeth that did not act as a support for a fixed prosthetic restoration; the favorable condition of the coronal restoration (p < 0.01). In the LRM, only the status of the coronal restoration was included. Preoperative conditions and the adequate fit of the coronal restoration influenced the outcome of RCT.
Uterus Transplantation with Live Donors: Screening Candidates in One French Center
Authors: Carbonnel Marie , Revaux Aurelie , Menzhulina Elena , Karpel Lea , Snanoudj Renaud , Le Guen Morgan , De Ziegler Dominique , Ayoubi Jean Marc
Published: June 2020

We report our experience regarding the profile and screening process of potential recipients (R) and their live donors (D) in our Uterus transplantation (UTx) trial from 2014 to 2020. The initial screening was performed using medical questionnaires and consultations. The second step of the screening consisted of two individual interviews with an independent multidisciplinary committee. Then, a complete medical, biological and imaging assessment of the directed living D, the R, and her partner was performed over a two-day hospitalization. A total of 239 women contacted our department: 165 potentials R and 74 potentials D. During the first step of screening, 141 R and 45 D were excluded. Only 12 R/D pairs were pursued. During inclusion, 10 R/D pairs were excluded. One R/D pair is still under evaluation. Finally, only 1 R/D pair was definitively included (0.6%), which led us to perform the first French UTx in March 2019 with a successful graft. The primary limiting factors of inclusion were due to very strict criteria and difficulty of having a suitable directed living D. The International Society of UTx (ISUTx) guidelines based on worldwide results of trials can help ease our inclusion criteria in the future while remaining safe for patients.
Risk Assessment of Work Accident in Container Terminals Using the Fault Tree Analysis Method
Authors: Budiyanto Muhammad Arif , Fernanda Haris
Published: June 2020

The use of containers in the world is increasing every year in line with international trade flows. In very complex container terminal operations, the risk of work accidents is inevitable and can happen at any time. Therefore, this paper aimed to identify accidents and potential risks occurring in the container terminals. For the case study, the analysis was used the data of accidents during five years in one of the major container terminals in Indonesia. Risk assessment is carried out using the risk matrix method to get the level of risk. The risk that has the highest level is analyzed by using the Fault Tree Analysis method. From the results of the risk assessment shows the container fell to the berth when loading and unloading have the highest risk value. While the results of the Fault Tree Analysis show that traffic accidents are the biggest potential risk, which is 41.8% compared to other accidents. Moreover, human factors especially due to the negligence in operating vehicles or equipment as well as the damage of equipment were the highest common causes of accidents in the container terminal. Based on these results the contribution offered is the risk control options for terminal operators to reduce the possibility of safety failure in the container terminal.
A 33.2 W High Beam Quality Chirped-Pulse Amplification-Based Femtosecond Laser for Industrial Processing
Authors: Bai Zhenao , Bai Zhenxu , Sun Xiaolong , Liang Yong , Wang Kun , Jin Duo , Fan Zhongwei
Published: June 2020

A photonic crystal fiber-based chirped pulse amplification delivering 272 fs pulses of 66.4 µJ energy at a repetition rate of 500 kHz is presented, resulting in an average/peak power of 33.2 W/244 MW. A single grating is adopted for the pulse width stretching and compression, which leads to high-compactness and low cost of the system. The output beam is near-diffraction-limited (M2 = 1.1 ± 0.05) with a power stability better than 0.5%. The cutting of alumina ceramic substrate and flexible printed circuit are demonstrated by using the laser system. The results indicate that the laser is competent for industrial applications.
The Effect of ZnO on the Failure of PET by Environmental Stress Cracking
Authors: Barros Ana Beatriz de Sousa , Farias Rômulo de Freitas , Siqueira Danilo Diniz , Luna Carlos Bruno Barreto , Araújo Edcleide Maria , Rabello Marcelo Silveira , Wellen Renate Maria Ramos
Published: June 2020

The aim of the present work is to evaluate the effect of NaOH solution as a stress cracking agent on the thermal and tensile properties of PET and PET/ZnO composites. The solutions were applied during tensile testing and the effects were monitored by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and testing the actual mechanical properties. The rate of crystallization was increased when the samples were exposed to NaOH, as observed by both cold and melt crystallization; this is possibly due to the reduction in molar mass of the PET molecules caused by NaOH. During melting, the DSC peaks became more complex, which is probably due to the distinct macromolecular mass, as well as crystallites with different sizes and levels of perfection. According to TGA analyses, no drastic changes were observed on the thermal stability of PET due to the action of NaOH. The tensile properties were shown to decrease drastically upon exposure to NaOH, which is the main symptom of stress cracking, leading to increased fragility, as also observed in the scanning electron microscopy (SEM) images. The presence of ZnO improved PET crystallization and provided some protection against the harmful effects of NaOH.
External Gas-Assisted Mold Temperature Control Improves Weld Line Quality in the Injection Molding Process
Authors: The Uyen Tran Minh , Truong Giang Nguyen , Do Thanh Trung , Anh Son Tran , Son Minh Pham
Published: June 2020

Simulations and experiments were conducted with gas temperatures of 200–400 °C to investigate the impact of external gas-assisted mold temperature control (Ex-GMTC) on the quality of weld line of molding products. In the heating step, the heating rate was 19.6 °C/s from 30 to 128.5 °C in the first 5 s in a 400 °C gas environment. When applied to heating the weld line area of an injection mold, Ex-GMTC improved the appearance of the weld line when the cavity temperature was preheated to 150 °C. For the tensile strength test, a melt flow simulation comparing the packing pressure of different mesh thicknesses revealed that Ex-GMTC helped maintain a high pressure in the weld line area in different packing periods. This was verified by an experiment where Ex-GMTC was applied with 400 °C gas to change the mesh area temperature. The result indicated that an increase in the weld line area temperature from 60 to 180 °C improves the tensile strength of all mesh thicknesses, which was more pronounced with thinner parts, especially at 0.4 mm. The simulations revealed that high temperature is concentrated in the weld line area of the cavity surface, thus reducing the energy wasted during heating.
Autologous Biologic Treatment with Fat, Bone Marrow Aspirate and Platelet Rich Plasma Is an Effective Alternative to Total Knee Arthroplasty for Patients with Moderate Knee Arthrosis
Authors: Prodromos Chadwick , Finkle Susan
Published: June 2020

Background: Osteoarthrosis (OA) of the knee afflicts millions worldwide. Total Knee Arthroplasty (TKA) is common, but associated with substantial cost and morbidity. Prior studies of intra-articular injection of fat, bone marrow aspirate (BMA), and platelet rich plasma (PRP) have shown clinical benefit. We hypothesized that injection of autologous adipose tissue, BMA, and PRP would provide significant benefit for patients with moderate knee OA resulting in avoidance of total knee arthroplasty (TKA) in most, with discontinuance of NSAIDs and other drugs. Methods: 42 TKA candidate patients (47 knees) with moderate (Kellgren-Lawrence 2 and 3) knee OA who had failed conservative treatment had autologous adipose tissue, BMA, and PRP injection as an alternative to TKA in office using only local anesthetic. Patients had discontinuance of all nonsteroidal anti-inflammatory medicines (NSAIDs) and other analgesics, except acetaminophen, prior to treatment. Patients were evaluated with Knee injury and Osteoarthritis Outcome Score Physical Shortform (KOOS-PS), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), and Single Assessment Numeric Evaluation (SANE) prior to treatment, and at 6 months, 1, and 2 years after treatment. Results: Follow up exceeded 80% at all time points. There were no significant adverse events. TKA was avoided in 97% at one and 86% at two years after treatment. Mean SANE, KOOS-PS, and WOMAC scores significantly improved at 6 months, 1, and 2 years post-treatment. WOMAC and SANE scores were higher at two versus one year post-treatment. Conclusions: Combined fat, BMA, and PRP injection is a safe and effective treatment for moderate knee OA, with reliable avoidance of TKA and possible continued improvement at two year follow-up.
Overview of Folic Acid Supplementation Alone or in Combination with Vitamin B12 in Dairy Cattle during Periparturient Period
Authors: Khan Muhammad Zahoor , Khan Adnan , Xiao Jianxin , Dou Jinhuan , Liu Lei , Yu Ying
Published: June 2020

The periparturient period is the period from three weeks before calving to three weeks post-calving. This period is important in terms of health, productivity and profitability, and is fundamental to successful lactation. During this period, the animal experiences stress because of hormonal changes due to pregnancy and the significant rise in milk production. In addition, a negative energy balance usually occurs, because the demand for nutrients to sustain milk production increases by more than the nutrient supply during the periparturient period. The immunity of dairy cattle is suppressed around parturition, which increases their susceptibility to infections. Special care regarding nutrition can reduce the risks of metabolism and immunity depression, which dairy cattle face during the periparturient span. Folic acid is relevant in this regard because of its critical role in the metabolism to maintain lactational performance and to improve health. Being a donor of one-carbon units, folic acid has a vital role in DNA and RNA biosynthesis. Generally, the folic acid requirements of dairy cattle can be met by the microbial synthesis in the rumen; however, in special cases, such as during the periparturient period, the requirement for this vitamin strictly increases. Vitamin B12 also has a critical role in the metabolism as a coenzyme of the enzyme methionine synthase for the transfer of a methyl group from folic acid to homocysteine for the regeneration of methionine. In the current review, we highlight the issues facing periparturient dairy cattle, and relevant knowledge and practices, and point out future research directions for utilization of the associated vitamins in ruminants, especially during the periparturient period.
Oxylipin Response to Acute and Chronic Exercise: A Systematic Review
Authors: Signini Étore F. , Nieman David C. , Silva Claudio D. , Sakaguchi Camila A. , Catai Aparecida M.
Published: June 2020

Oxylipins are oxidized compounds of polyunsaturated fatty acids that play important roles in the body. Recently, metabololipidomic-based studies using advanced mass spectrometry have measured the oxylipins generated during acute and chronic physical exercise and described the related physiological effects. The objective of this systematic review was to provide a panel of the primary exercise-related oxylipins and their respective functions in healthy individuals. Searches were performed in five databases (Cochrane, PubMed, Science Direct, Scopus and Web of Science) using combinations of the Medical Subject Headings (MeSH) terms: “Humans”, “Exercise”, “Physical Activity”, “Sports”, “Oxylipins”, and “Lipid Mediators”. An adapted scoring system created in a previous study from our group was used to rate the quality of the studies. Nine studies were included after examining 1749 documents. Seven studies focused on the acute effect of physical exercise while two studies determined the effects of exercise training on the oxylipin profile. Numerous oxylipins are mobilized during intensive and prolonged exercise, with most related to the inflammatory process, immune function, tissue repair, cardiovascular and renal functions, and oxidative stress.
Vibrio harveyi Infection Significantly Alters Amino Acid and Carbohydrate Metabolism in Whiteleg Shrimp, Litopenaeus vannamei
Authors: Ma Seohee , Kim Ahran , Lee Wonho , Kim Seonghye , Lee Sujin , Yoon Dahye , Bae Jin-Sol , Park Chan-Il , Kim Suhkmann
Published: June 2020

Vibrio harveyi is one of the pathogens that threaten the shrimp farming industry. However, metabolic changes induced by V. harveyi infection in shrimp remain unknown. In this study, we first conducted high resolution-magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR)-based metabolomics studies on gill, hepatopancreas, and haemolymph of V. harveyi-infected white leg shrimp, Litopenaeus vannamei. Using multivariate statistical analysis, we observed a clear separation between the early (3 and 9 h post-injection (hpi)) and late phases (24, 72 and 144 hpi) of the infection in all tissues. Moreover, metabolic changes in response to V. harveyi infection were faster in the haemolymph in the early phase and significantly changed in the late phase of the infection in the gills. Extensive changes were observed in the hepatopancreas, with 24 hpi being the turning point of progression from early to late phase infection in the hepatopancreas. V. harveyi infection increased the energy demand in L. vannamei and the amino acid and carbohydrate metabolism pathways also exhibited significant changes depending on the tissue. Thus, each tissue displayed different metabolic changes, depending on the progress of the infection.
The Mechanical and Physical Properties of 3D-Printed Materials Composed of ABS-ZnO Nanocomposites and ABS-ZnO Microcomposites
Authors: Vidakis Nectarios , Petousis Markos , Maniadi Athena , Koudoumas Emmanuel , Kenanakis George , Romanitan Cosmin , Tutunaru Oana , Suchea Mirela , Kechagias John
Published: June 2020

In order to expand the mechanical and physical capabilities of 3D-printed structures fabricated via commercially available 3D printers, nanocomposite and microcomposite filaments were produced via melt extrusion, 3D-printed and evaluated. The scope of this work is to fabricate physically and mechanically improved nanocomposites or microcomposites for direct commercial or industrial implementation while enriching the existing literature with the methodology applied. Zinc Oxide nanoparticles (ZnO nano) and Zinc Oxide micro-sized particles (ZnO micro) were dispersed, in various concentrations, in Acrylonitrile Butadiene Styrene (ABS) matrices and printable filament of ~1.75mm was extruded. The composite filaments were employed in a commercial 3D printer for tensile and flexion specimens’ production, according to international standards. Results showed a 14% increase in the tensile strength at 5% wt. concentration in both nanocomposite and microcomposite materials, when compared to pure ABS specimens. Furthermore, a 15.3% increase in the flexural strength was found in 0.5% wt. for ABS/ZnO nano, while an increase of 17% was found on 5% wt. ABS/ZnO micro. Comparing the two composites, it was found that the ABS/ZnO microcomposite structures had higher overall mechanical strength over ABS/ZnO nanostructures.
Late Paleozoic–Early Mesozoic Granite Magmatism on the Arctic Margin of the Siberian Craton during the Kara-Siberia Oblique Collision and Plume Events
Authors: Vernikovsky Valery , Vernikovskaya Antonina , Proskurnin Vasilij , Matushkin Nikolay , Proskurnina Maria , Kadilnikov Pavel , Larionov Alexander , Travin Alexey
Published: June 2020

We present new structural, petrographic, geochemical and geochronological data for the late Paleozoic–early Mesozoic granites and associated igneous rocks of the Taimyr Peninsula. It is demonstrated that large volumes of granites were formed due to the oblique collision of the Kara microcontinent and the Siberian paleocontinent. Based on U-Th-Pb isotope data for zircons, we identify syncollisional (315–282 Ma) and postcollisional (264–248 Ma) varieties, which differ not only in age but also in petrochemical and geochemical features. It is also shown that as the postcollisional magmatism was coming to an end, Siberian plume magmatism manifested in the Kara orogen and was represented by basalts and dolerites of the trap formation (251–249 Ma), but also by differentiated and individual intrusions of monzonites, quartz monzonites and syenites (Early–Middle Triassic) with a mixed crustal-mantle source. We present a geodynamic model for the formation of the Kara orogen and discuss the relationship between collisional and trap magmatism.
Comparative Effects of Dietary Hemp and Poppy Seed Oil on Lipid Metabolism and the Antioxidant Status in Lean and Obese Zucker Rats
Authors: Fotschki Bartosz , Opyd Paulina , Juśkiewicz Jerzy , Wiczkowski Wiesław , Jurgoński Adam
Published: June 2020

The objective of this study was to compare the effects of the dietary inclusion of hemp seed oil (HO) and poppy seed oil (PO) on the lipid metabolism and antioxidant status of lean and genetically obese Zucker rats. The rats were fed a control diet for laboratory rodents or a modification with HO or PO. Both oils reduced body and epididymal fat and liver cholesterol levels and promoted oxidative stress in the liver of obese rats. The HO reduced plasma triglycerides and had a stronger liver cholesterol-lowering effect in obese rats than PO. In the lean rats, HO and PO had no effects on the body fat content, plasma lipid profile, or lipid metabolism in the liver. HO considerably elevated the content of α-linolenic acid in the liver and increased the liver ratio of reduced glutathione (GSH)/oxidized glutathione (GSSG) in the lean rats. In conclusion, the regular consumption of both oils increases the accumulation of essential fatty acids in the liver of healthy animals, whilst not having any adverse effects on the body, whereas in genetically obese rats, the effects of both dietary oils on the lipid metabolism and antioxidant status are unequivocal and only partially beneficial.
DNA Photocleavage in the Near-Infrared Wavelength Range by 2-Quinolinium Dicarbocyanine Dyes
Authors: Ahoulou Effibe O. , Drinkard Kaitlyn K. , Basnet Kanchan , St. Lorenz Anna , Taratula Oleh , Henary Maged , Grant Kathryn B.
Published: June 2020

Here, we report the syntheses of two pentamethine cyanine dyes containing quinolinium rings and substituted with either hydrogen (3) or bromine (4) at the meso carbon. The electron withdrawing bromine atom stabilizes dye 4 in aqueous buffer, allowing complex formation to occur between the dye and double-helical DNA. UV–visible, CD, and fluorescence spectra recorded at low DNA concentrations suggest that dye 4 initially binds to the DNA as a high-order aggregate. As the ratio of DNA to dye is increased, the aggregate is converted to monomeric and other low-order dye forms that interact with DNA in a non-intercalative fashion. The brominated dye 4 is relatively unreactive in the dark, but, under 707–759 nm illumination, generates hydroxyl radicals that cleave DNA in high yield (pH 7.0, 22 °C). Dye 4 is also taken up by ES2 ovarian carcinoma cells, where it is non-toxic under dark conditions. Upon irradiation of the ES2 cells at 694 nm, the brominated cyanine reduces cell viability from 100 ± 10% to 14 ± 1%. Our results suggest that 2-quinolinium-based carbocyanine dyes equipped with stabilizing electron withdrawing groups may have the potential to serve as sensitizing agents in long-wavelength phototherapeutic applications.
Sustainable Palm Oil—The Role of Screening and Advanced Analytical Techniques for Geographical Traceability and Authenticity Verification
Authors: Ramli Umi Salamah , Tahir Noor Idayu , Rozali Nurul Liyana , Othman Abrizah , Muhammad Nor Hayati , Muhammad Syahidah Akmal , Tarmizi Azmil Haizam Ahmad , Hashim Norfadilah , Sambanthamurthi Ravigadevi , Singh Rajinder , Manaf Mohamad Arif Abd , Parveez Ghulam Kadir Ahmad
Published: June 2020

Palm oil production from oil palm (Elaeis guineensis Jacq.) is vital for the economy of Malaysia. As of late, sustainable production of palm oil has been a key focus due to demand by consumer groups, and important progress has been made in establishing standards that promote good agricultural practices that minimize impact on the environment. In line with the industrial goal to build a traceable supply chain, several measures have been implemented to ensure that traceability can be monitored. Although the palm oil supply chain can be highly complex, and achieving full traceability is not an easy task, the industry has to be proactive in developing improved systems that support the existing methods, which rely on recorded information in the supply chain. The Malaysian Palm Oil Board (MPOB) as the custodian of the palm oil industry in Malaysia has taken the initiative to assess and develop technologies that can ensure authenticity and traceability of palm oil in the major supply chains from the point of harvesting all the way to key downstream applications. This review describes the underlying framework related to palm oil geographical traceability using various state-of-the-art analytical techniques, which are also being explored to address adulteration in the global palm oil supply chain.
Antifungal Agent 4-AN Changes the Genome-Wide Expression Profile, Downregulates Virulence-Associated Genes and Induces Necrosis in Candida albicans Cells
Authors: Martyna Aleksandra , Masłyk Maciej , Janeczko Monika , Kochanowicz Elżbieta , Gielniewski Bartłomiej , Świercz Aleksandra , Demchuk Oleg M. , Kubiński Konrad
Published: June 2020

In the light of the increasing occurrence of antifungal resistance, there is an urgent need to search for new therapeutic strategies to overcome this phenomenon. One of the applied approaches is the synthesis of small-molecule compounds showing antifungal properties. Here we present a continuation of the research on the recently discovered anti-Candida albicans agent 4-AN. Using next generation sequencing and transcriptional analysis, we revealed that the treatment of C. albicans with 4-AN can change the expression profile of a large number of genes. The highest upregulation was observed in the case of genes involved in cell stress, while the highest downregulation was shown for genes coding sugar transporters. Real-time PCR analysis revealed 4-AN mediated reduction of the relative expression of genes engaged in fungal virulence (ALS1, ALS3, BCR1, CPH1, ECE1, EFG1, HWP1, HYR1 and SAP1). The determination of the fractional inhibitory concentration index (FICI) showed that the combination of 4-AN with amphotericin B is synergistic. Finally, flow cytometry analysis revealed that the compound induces mainly necrosis in C. albicans cells.
The (+)-Brevipolide H Displays Anticancer Activity against Human Castration-Resistant Prostate Cancer: The Role of Oxidative Stress and Akt/mTOR/p70S6K-Dependent Pathways in G1 Checkpoint Arrest and Apoptosis
Authors: Sheng Yi-Hua , Leu Wohn-Jenn , Chen Ching-Nung , Hsu Jui-Ling , Liu Ying-Tung , Hsu Lih-Ching , Hou Duen-Ren , Guh Jih-Hwa
Published: June 2020

Because conventional chemotherapy is not sufficiently effective against prostate cancer, various examinations have been performed to identify anticancer activity of naturally occurring components and their mechanisms of action. The (+)-brevipolide H, an α-pyrone-based natural compound, induced potent and long-term anticancer effects in human castration-resistant prostate cancer (CRPC) PC-3 cells. Flow cytofluorometric analysis with propidium iodide staining showed (+)-brevipolide H-induced G1 arrest of cell cycle and subsequent apoptosis through induction of caspase cascades. Since Akt/mTOR pathway has been well substantiated in participating in cell cycle progression in G1 phase, its signaling and downstream regulators were examined. Consequently, (+)-brevipolide H inhibited the signaling pathway of Akt/mTOR/p70S6K. The c-Myc inhibition and downregulation of G1 phase cyclins were also attributed to (+)-brevipolide H action. Overexpression of myristoylated Akt significantly rescued mTOR/p70S6K and downstream signaling under (+)-brevipolide H treatment. ROS and Ca2+, two key mediators in regulating intracellular signaling, were determined, showing that (+)-brevipolide H interactively induced ROS production and an increase of intracellular Ca2+ levels. The (+)-Brevipolide H also induced the downregulation of anti-apoptotic Bcl-2 family proteins (Bcl-2 and Bcl-xL) and loss of mitochondrial membrane potential, indicating the contribution of mitochondrial dysfunction to apoptosis. In conclusion, the data suggest that (+)-brevipolide H displays anticancer activity through crosstalk between ROS production and intracellular Ca2+ mobilization. In addition, suppression of Akt/mTOR/p70S6K pathway associated with downregulation of G1 phase cyclins contributes to (+)-brevipolide H-mediated anticancer activity, which ultimately causes mitochondrial dysfunction and cell apoptosis. The data also support the biological significance and, possibly, clinically important development of natural product-based anticancer approaches.
Green and Sustainable Valorization of Bioactive Phenolic Compounds from Pinus By-Products
Authors: Ferreira-Santos Pedro , Zanuso Elisa , Genisheva Zlatina , Rocha Cristina M. R. , Teixeira José A.
Published: June 2020

In Europe, pine forests are one of the most extended forests formations, making pine residues and by-products an important source of compounds with high industrial interest as well as for bioenergy production. Moreover, the valorization of lumber industry residues is desirable from a circular economy perspective. Different extraction methods and solvents have been used, resulting in extracts with different constituents and consequently with different bioactivities. Recently, emerging and green technologies as ultrasounds, microwaves, supercritical fluids, pressurized liquids, and electric fields have appeared as promising tools for bioactive compounds extraction in alignment with the Green Chemistry principles. Pine extracts have attracted the researchers’ attention because of the positive bioproperties, such as anti-inflammatory, antimicrobial, anti-neurodegenerative, antitumoral, cardioprotective, etc., and potential industrial applications as functional foods, food additives as preservatives, nutraceuticals, pharmaceuticals, and cosmetics. Phenolic compounds are responsible for many of these bioactivities. However, there is not much information in the literature about the individual phenolic compounds of extracts from the pine species. The present review is about the reutilization of residues and by-products from the pine species, using ecofriendly technologies to obtain added-value bioactive compounds for industrial applications.
Comparative Study of Different Acidic Surface Structures in Solid Catalysts Applied for the Isobutene Dimerization Reaction
Authors: Fernández-Morales José M. , Castillejos Eva , Asedegbega-Nieto Esther , Dongil Ana Belén , Rodríguez-Ramos Inmaculada , Guerrero-Ruiz Antonio
Published: June 2020

Dimerization of isobutene (IBE) to C8s olefins was evaluated over a range of solid acid catalysts of diverse nature, in a fixed bed reactor working in a continuous mode. All catalytic materials were studied in the title reaction performed between 50–250 °C, being the reaction feed a mixture of IBE/helium (4:1 molar ratio). In all materials, both conversion and selectivity increased with increasing reaction temperature and at 180 °C the best performance was recorded. Herein, we used thermogravimetry analysis (TGA) and temperature programmed desorption of adsorbed ammonia (NH3-TPD) for catalysts characterization. We place emphasis on the nature of acid sites that affect the catalytic performance. High selectivity to C8s was achieved with all catalysts. Nicely, the catalyst with higher loading of Brønsted sites displayed brilliant catalytic performance in the course of the reaction (high IBE conversion). However, optimum selectivity towards C8 compounds led to low catalyst stability, this being attributed to the combined effect between the nature of acidic sites and structural characteristics of the catalytic materials used. Therefore, this study would foment more research in the optimization of the activity and the selectivity for IBE dimerization reactions.
Effectiveness of Interventions to Promote Healthy Eating Habits in Children and Adolescents at Risk of Poverty: Systematic Review and Meta-Analysis
Authors: Pastor Rosario , Tur Josep A.
Published: June 2020

The objective of this review was to provide an up-to-date review of trials that include behavioral intervention on the eating habits of children and adolescents at risk of poverty, applying meta-analysis to estimate the size of the intervention effect. A systematic literature search was performed in the following databases: MEDLINE via Pubmed and via EBSCOhost, LILACS and IBECS via VHL. The MeSH terms were used: “social class”, “poverty”, “diet”, “health promotion” (PROSPERO ID: 183900). A total of 14 articles were finally included in this systematic review. The primary results of the included studies were meta-analyzed with the RevMan 5.3 program, assuming a random effects model. Analysis of the overall effect showed combined standardized mean differences (SMD) of 0.16 (CI 95%: [0.03, 0.30], I2 = 76%). The p-value for the Z statistic was 0.02, which reflects accuracy in the effect estimation. When establishing subgroups based on the intervention tracking time points, studies that provided longer-term follow-up data showed a combined SMD of 0.15 (CI 95%: [−0.02, 0.32], I2 = 81%]). Small effects of behavioral interventions were found to promote healthy eating habits, but better effects were shown in cases where the invention was followed up in the long term.
Optics to the World
Authors: De Angelis Costantino
Published: June 2020

Optics play a vital role in today’s society [...]
Changes in miR-122 and Cholesterol Expression in Chronic Hepatitis C Patients after PegIFN-Alpha/Ribavirin Treatment
Authors: Sidorkiewicz Malgorzata , Grek-Kowalinska Martyna , Piekarska Anna
Published: June 2020

The hepatitis C virus (HCV) is known as a main etiological cause of chronic hepatitis. HCV infection disturbs cholesterol metabolism of the host, which is frequently observed in patients suffering from chronic hepatitis C (CHC). The course of viral infections remains under strict control of microRNA (miRNA). In the case of HCV, miR-122 exerts a positive effect on HCV replication in vitro. The purpose of this study was to investigate the impact of peginterferon alpha (pegIFN-α) and ribavirin treatments on the expression of miR-122 and the cholesterol level in the peripheral blood mononuclear cells (PBMCs) of CHC patients. We report here that the level of miR-122 expression in the PBMCs decreased after the antiviral treatment in comparison to the pretreated state. Simultaneously, the level of cholesterol in the PBMCs of CHC patients was higher six months following the treatment than it was pretreatment. Consequently, it seems that the decrease of miR-122 expression in the PBMCs of CHC patients is one of the antiviral effects connected with the pegIFN-alpha/ribavirin treatments.
Physiological Basis of Salt Stress Tolerance in a Landrace and a Commercial Variety of Sweet Pepper (Capsicum annuum L.)
Authors: Giorio Pasquale , Cirillo Valerio , Caramante Martina , Oliva Marco , Guida Gianpiero , Venezia Accursio , Grillo Stefania , Maggio Albino , Albrizio Rossella
Published: June 2020

Salt stress is one of the most impactful abiotic stresses that plants must cope with. Plants’ ability to tolerate salt stress relies on multiple mechanisms, which are associated with biomass and yield reductions. Sweet pepper is a salt-sensitive crop that in Mediterranean regions can be exposed to salt build-up in the root zone due to irrigation. Understanding the physiological mechanisms that plants activate to adapt to soil salinization is essential to develop breeding programs and agricultural practices that counteract this phenomenon and ultimately minimize yield reductions. With this aim, the physiological and productive performances of Quadrato D’Asti, a common commercial sweet pepper cultivar in Italy, and Cazzone Giallo, a landrace of the Campania region (Italy), were compared under different salt stress treatments. Quadrato D’Asti had higher tolerance to salt stress when compared to Cazzone Giallo in terms of yield, which was associated with higher leaf biomass vs. fruit ratio in the former. Ion accumulation and profiling between the two genoptypes revealed that Quadrato D’Asti was more efficient at excluding chloride from green tissues, allowing the maintenance of photosystem functionality under stress. In contrast, Cazzone Giallo seemed to compartmentalize most sodium in the stem. While sodium accumulation in the stems has been shown to protect shoots from sodium toxicity, in pepper and/or in the specific experimental conditions imposed, this strategy was less efficient than chloride exclusion for salt stress tolerance.
Erratum: Facile Functionalization via Plasma-Enhanced Chemical Vapor Deposition for the Effective Filtration of Oily Aerosol. Polymers 2019, 11, 1490
Authors: Roh Sanghyun , Kim Sungmin , Kim Jooyoun
Published: June 2020

The authors wish to make a change to the published paper [...]
Uncertainties in CERES Top-of-Atmosphere Fluxes Caused by Changes in Accompanying Imager
Authors: Su Wenying , Liang Lusheng , Wang Hailan , Eitzen Zachary A.
Published: June 2020

The Clouds and the Earth’s Radiant Energy System (CERES) project provides observations of Earth’s radiation budget using measurements from CERES instruments on board the Terra, Aqua, Suomi National Polar-orbiting Partnership (S-NPP), and NOAA-20 satellites. The CERES top-of-atmosphere (TOA) fluxes are produced by converting radiance measurements using empirical angular distribution models, which are functions of cloud properties that are retrieved from imagers flying with the CERES instruments. As the objective is to create a long-term climate data record, not only calibration consistency of the six CERES instruments needs to be maintained for the entire time period, it is also important to maintain the consistency of other input data sets used to produce this climate data record. In this paper, we address aspects that could potentially affect the CERES TOA flux data quality. Discontinuities in imager calibration can affect cloud retrieval which can lead to erroneous flux trends. When imposing an artificial 0.6 per decade decreasing trend to cloud optical depth, which is similar to the trend difference between CERES Edition 2 and Edition 4 cloud retrievals, the decadal SW flux trend changed from − 0.3 5 ± 0.18 Wm − 2 to 0.61 ± 0.18 Wm − 2 . This indicates that a 13% change in cloud optical depth results in about 1% change in the SW flux. Furthermore, different CERES instruments provide valid fluxes at different viewing zenith angle ranges, and including fluxes derived at the most oblique angels unique to S-NPP (>66 ∘ ) can lead to differences of 0.8 Wm − 2 and 0.3 Wm − 2 in global monthly mean instantaneous SW flux and LW flux. To ensure continuity, the viewing zenith angle ranges common to all CERES instruments (<66 ∘ ) are used to produce the long-term Earth’s radiation budget climate data record. The consistency of cloud properties retrieved from different imagers also needs to be maintained to ensure the TOA flux consistency.
New Town Development and Sustainable Transition under Urban Entrepreneurialism in China
Authors: Song Yun , Stead Dominic , de Jong Martin
Published: June 2020

New towns are a major form of urban growth in China. In recent years, increasing numbers of large new town projects have been planned and built in and around existing cities. These new town projects have frequently been employed by city governments as central elements of pro-growth strategies, based on ideas of urban entrepreneurialism, which seek to promote economic growth, project a dynamic city image, and increase urban competitiveness. This article studies how the pro-growth, urban entrepreneurial approach affects the planning and development of Chinese megacities. A conceptual framework focusing on land-leasing revenue and new town development strategies is employed to explore the linkages between urban growth mechanisms and urban outcomes. Empirical material from four cities in the Pearl River Delta—Guangzhou, Shenzhen, Foshan, and Zhuhai—is presented. The analysis indicates that new town developments in these cities have different levels of dependency on spatial expansion and land revenue, and emphasize different issues of sustainable development in their plans. Cities with a lower dependency on physical and economic growth are be more likely to emphasize the quality of the built environment and address issues of sustainable urban development more closely when planning and implementing new town projects.
Going Vegan: The Role(s) of ICT in Vegan Practice Transformation
Authors: Lawo Dennis , Esau Margarita , Engelbutzeder Philip , Stevens Gunnar
Published: June 2020

With the debate on climate change, topics of diet change and the reduction of animal products have become increasingly important in both public and academic discourses. However, sustainable ICT studies have so far focused on individual aspects, in particular investigating the criticized persuasive design approach. We argue for a broader perspective on the role(s) of ICT, one that helps in identifying opportunities to support consumer practice transformation, beyond motivational aspects. Based on retrospective interviews with 16 vegans, we argue to understand practice transformation as co-evolution of practices and ICT artefacts, as this perspective helps to understand how tensions arising from complex entanglements of practices, socio-material contexts, and communities can be resolved. Rather than a motivational process, we observe various roles of ICT artefacts co-evolving with practices: Ranging from initial irritation, to access to information about vegan practices, to the learning of vegan food literacy, to the negotiation of a vegan identity, and vegan norms at the intersection of the ‘odd’ and the ‘norm’.