bluuebunny/crossref_metadata_2025 huggingface dataset:
Surface Micro Discharge–Cold Atmospheric Pressure Plasma Processing of Common House Cricket Acheta domesticus Powder: Antimicrobial Potential and Lipid-Quality Preservation
Authors: Pina-Pérez Maria C. , Rodrigo Dolores , Ellert Christoph , Beyrer Michael
Published: July 2021

The growing world population and the need to reduce the environmental impact of food production drive the exploration of novel protein sources. Insects are being cultivated, harvested, and processed to be applied in animal and human nutrition. The inherent microbial contamination of insect matrices requires risk management and decontamination strategies. Thermal sterilization results in unfavorable cooking effects and oxidation of fatty acids. The present study demonstrates the risk management in Acheta domesticus (home cricket) powder with a low-energy (8.7–22.0 mW/cm2, 5 min) semi-direct surface micro discharge (SMD)–cold atmospheric pressure plasma (CAPP). At a plasma power density lower than 22 mW/cm2, no degradation of triglycerides (TG) or increased free fatty acids (FFA) content was detected. For mesophilic bacteria, 1.6 ± 0.1 log10 reductions were achieved, and for Enterobacteriaceae, there were close to 1.9 ± 0.2 log10 reductions in a layer of powder. Colonies of Bacillus cereus, Bacillus subtilis, and Bacillus megaterium were identified via the mass spectral fingerprint analyzed with matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS). The spores of these Bacillus strains resisted to a plasma power density of 22 mW/cm2. Additional inactivation effects at non-thermal, practically non-oxidative conditions are supposed for low-intensity plasma treatments combined with the powder’s fluidization.
Pyrroline-5-Carboxylate Reductase 1 Directs the Cartilage Protective and Regenerative Potential of Murphy Roths Large Mouse Mesenchymal Stem Cells
Authors: Tejedor Gautier , Contreras-Lopez Rafael , Barthelaix Audrey , Ruiz Maxime , Noël Danièle , De Ceuninck Frédéric , Pastoureau Philippe , Luz-Crawford Patricia , Jorgensen Christian , Djouad Farida
Published: July 2021

Murphy Roths Large (MRL) mice possess outstanding capacity to regenerate several tissues. In the present study, we investigated whether this regenerative potential could be associated with the intrinsic particularities possessed by their mesenchymal stem cells (MSCs). We demonstrated that MSCs derived from MRL mice (MRL MSCs) display a superior chondrogenic potential than do C57BL/6 MSC (BL6 MSCs). This higher chondrogenic potential of MRL MSCs was associated with a higher expression level of pyrroline-5-carboxylate reductase 1 (PYCR1), an enzyme that catalyzes the biosynthesis of proline, in MRL MSCs compared with BL6 MSCs. The knockdown of PYCR1 in MRL MSCs, using a specific small interfering RNA (siRNA), abolishes their chondrogenic potential. Moreover, we showed that PYCR1 silencing in MRL MSCs induced a metabolic switch from glycolysis to oxidative phosphorylation. In two in vitro chondrocyte models that reproduce the main features of osteoarthritis (OA) chondrocytes including a downregulation of chondrocyte markers, a significant decrease of PYCR1 was observed. A downregulation of chondrocyte markers was also observed by silencing PYCR1 in freshly isolated healthy chondrocytes. Regarding MSC chondroprotective properties on chondrocytes with OA features, we showed that MSCs silenced for PYCR1 failed to protect chondrocytes from a reduced expression of anabolic markers, while MSCs overexpressing PYCR1 exhibited an increased chondroprotective potential. Finally, using the ear punch model, we demonstrated that MRL MSCs induced a regenerative response in non-regenerating BL6 mice, while BL6 and MRL MSCs deficient for PYCR1 did not. In conclusion, our results provide evidence that MRL mouse regenerative potential is, in part, attributed to its MSCs that exhibit higher PYCR1-dependent glycolytic potential, differentiation capacities, chondroprotective abilities, and regenerative potential than BL6 MSCs.
Nanoparticles for Stem Cell Tracking and the Potential Treatment of Cardiovascular Diseases
Authors: Huang Huihua , Du Xuejun , He Zhiguo , Yan Zifeng , Han Wei
Published: July 2021

Stem cell-based therapies have been shown potential in regenerative medicine. In these cells, mesenchymal stem cells (MSCs) have the ability of self-renewal and being differentiated into different types of cells, such as cardiovascular cells. Moreover, MSCs have low immunogenicity and immunomodulatory properties, and can protect the myocardium, which are ideal qualities for cardiovascular repair. Transplanting mesenchymal stem cells has demonstrated improved outcomes for treating cardiovascular diseases in preclinical trials. However, there still are some challenges, such as their low rate of migration to the ischemic myocardium, low tissue retention, and low survival rate after the transplantation. To solve these problems, an ideal method should be developed to precisely and quantitatively monitor the viability of the transplanted cells in vivo for providing the guidance of clinical translation. Cell imaging is an ideal method, but requires a suitable contrast agent to label and track the cells. This article reviews the uses of nanoparticles as contrast agents for tracking MSCs and the challenges of clinical use of MSCs in the potential treatment of cardiovascular diseases.
PLXNC1: A Novel Potential Immune-Related Target for Stomach Adenocarcinoma
Authors: Ni Zhizhan , Huang Chenshen , Zhao Hongmei , Zhou Jinzhe , Hu Muren , Chen Qing , Ge Bujun , Huang Qi
Published: July 2021

Background

Gastric cancer is associated with tumor microenvironment and chronic inflammation, but the underlying tumor-promoting mechanisms still remain unknown.

Methods

The ATAC-seq was used to identify genes with chromatin accessibilities in promoter regions. The RNA-seq datasets were performed to identify differentially expressed genes (DEGs). Pearson correlation analysis with the mRNA expression of three families of tumor-related inflammation TFs was used to filter downstream DEGs. Cox univariate survival analysis was performed to identify the prognostic value. The ImmPort database and CIBERSORTx algorithm were used to investigate the regulatory relationship between hub DEGs and immune cells. Immunohistochemistry (IHC) and multidimensional database were performed to verification.

Results

In this case, we require 2,454 genes with chromatin accessibility in promoter regions by ATAC-seq. Based on the gene expression profiles (RNA-seq), we identified 365 genes with chromatin accessibility and differential expression. Combined with the Cox univariate survival analysis, we identified 32 survival-related DEGs with chromatin accessibility. According to ImmPort database, CXCL3, PLXNC1, and EDN2 were identified as immune- related genes in STAD. By applying the CIBERSORTx algorithm and Pearson correlation, PLXNC1 was the only gene correlated with various immune cells, significantly associated with M2 macrophages. Furthermore, gene set variation analysis (GSVA) suggests the “hallmark_interferon_gamma_response” pathway was most significantly correlated with PLXNC1. Immunohistochemistry results revealed that PLXNC1 protein level was significantly higher in STAD tissues than in normal tissues (p < 0.001).

Conclusion

PLXNC1, regulated by IRF5, is an immune-related gene that was significantly associated with M2 macrophages and poor outcome in stomach adenocarcinoma.
The Genomic Response to TGF-β1 Dictates Failed Repair and Progression of Fibrotic Disease in the Obstructed Kidney
Authors: Higgins Craig E. , Tang Jiaqi , Higgins Stephen P. , Gifford Cody C. , Mian Badar M. , Jones David M. , Zhang Wenzheng , Costello Angelica , Conti David J. , Samarakoon Rohan , Higgins Paul J.
Published: July 2021

Tubulointerstitial fibrosis is a common and diagnostic hallmark of a spectrum of chronic renal disorders. While the etiology varies as to the causative nature of the underlying pathology, persistent TGF-β1 signaling drives the relentless progression of renal fibrotic disease. TGF-β1 orchestrates the multifaceted program of kidney fibrogenesis involving proximal tubular dysfunction, failed epithelial recovery or re-differentiation, capillary collapse and subsequent interstitial fibrosis eventually leading to chronic and ultimately end-stage disease. An increasing complement of non-canonical elements function as co-factors in TGF-β1 signaling. p53 is a particularly prominent transcriptional co-regulator of several TGF-β1 fibrotic-response genes by complexing with TGF-β1 receptor-activated SMADs. This cooperative p53/TGF-β1 genomic cluster includes genes involved in cellular proliferative control, survival, apoptosis, senescence, and ECM remodeling. While the molecular basis for this co-dependency remains to be determined, a subset of TGF-β1-regulated genes possess both p53- and SMAD-binding motifs. Increases in p53 expression and phosphorylation, moreover, are evident in various forms of renal injury as well as kidney allograft rejection. Targeted reduction of p53 levels by pharmacologic and genetic approaches attenuates expression of the involved genes and mitigates the fibrotic response confirming a key role for p53 in renal disorders. This review focuses on mechanisms underlying TGF-β1-induced renal fibrosis largely in the context of ureteral obstruction, which mimics the pathophysiology of pediatric unilateral ureteropelvic junction obstruction, and the role of p53 as a transcriptional regulator within the TGF-β1 repertoire of fibrosis-promoting genes.
m6A Regulator-Associated Modification Patterns and Immune Infiltration of the Tumor Microenvironment in Hepatocarcinoma
Authors: Li Jianhao , Wang Weiwei , Zhou Yubing , Liu Liwen , Zhang Guizhen , Guan Kelei , Cui Xichun , Liu Xin , Huang Maoxin , Cui Guangying , Sun Ranran
Published: July 2021

Background: Immunotherapy elicits durable responses in many tumors. Nevertheless, the positive response to immunotherapy always depends on the dynamic interactions between the tumor cells and infiltrating lymphocytes in the tumor microenvironment (TME). Currently, the application of immunotherapy in hepatocellular carcinoma (HCC) has achieved limited success. The ectopic modification of N6-methyladenosine (m6A) is a common feature in multiple tumors. However, the relationship between m6A modification with HCC clinical features, prognosis, immune cell infiltration, and immunotherapy efficacy remains unclear.

Materials and Methods: Here, we comprehensively evaluated m6A modification clusters based on 22 m6A regulators and systematically explored the relationship between m6A modification with tumor progression, prognosis, and immune cell infiltration characteristics. The m6Ascore was calculated by principal component analysis to quantify the m6A modifications of individual patients. Key regulators involved in immunoregulation in HCC were identified using immunohistochemistry and immunofluorescence.

Results: Three distinct m6A modification clusters were identified. The m6A clusters were significantly associated with clinical features, prognosis, and immune cell infiltration. The three clusters were highly consistent with the three tumor immune phenotypes, i.e., immune-excluded, immune-inflamed, and immune-desert. Comprehensive bioinformatics analysis revealed that high m6Ascore was closely associated with tumor progression, poor prognosis, and immunotherapy non-response. m6A regulators were dysregulated in HCC tissues. Hence, they play a role as predictors of poor prognosis. Tissue microarray demonstrated that overexpressed YTHDF1 was associated with low CD3+ and CD8+ T cell infiltration in HCC.

Conclusion: Our findings demonstrate that m6A modification patterns play a crucial role in the tumor immune microenvironment and the prognosis of HCC. High YTHDF1 expression is closely associated with low CD3+ and CD8+ T cell infiltration in HCC.
Modular Evolution and Population Variability of Oikopleura dioica Metallothioneins
Authors: Calatayud Sara , Garcia-Risco Mario , Capdevila Mercè , Cañestro Cristian , Palacios Òscar , Albalat Ricard
Published: July 2021

Chordate Oikopleura dioica probably is the fastest evolving metazoan reported so far, and thereby, a suitable system in which to explore the limits of evolutionary processes. For this reason, and in order to gain new insights on the evolution of protein modularity, we have investigated the organization, function and evolution of multi-modular metallothionein (MT) proteins in O. dioica. MTs are a heterogeneous group of modular proteins defined by their cysteine (C)-rich domains, which confer the capacity of coordinating different transition metal ions. O. dioica has two MTs, a bi-modular OdiMT1 consisting of two domains (t-12C and 12C), and a multi-modular OdiMT2 with six t-12C/12C repeats. By means of mass spectrometry and spectroscopy of metal-protein complexes, we have shown that the 12C domain is able to autonomously bind four divalent metal ions, although the t-12C/12C pair –as it is found in OdiMT1– is the optimized unit for divalent metal binding. We have also shown a direct relationship between the number of the t-12C/12C repeats and the metal-binding capacity of the MTs, which means a stepwise mode of functional and structural evolution for OdiMT2. Finally, after analyzing four different O. dioica populations worldwide distributed, we have detected several OdiMT2 variants with changes in their number of t-12C/12C domain repeats. This finding reveals that the number of repeats fluctuates between current O. dioica populations, which provides a new perspective on the evolution of domain repeat proteins.
Formaldehyde Analysis in Non-Aqueous Methanol Solutions by Infrared Spectroscopy and Electrospray Ionization
Authors: Barakoti Krishna K. , Subedi Pradeep , Chalyavi Farzaneh , Gutierrez-Portocarrero Salvador , Tucker Matthew J. , Alpuche-Aviles Mario A.
Published: July 2021

We present the analysis of formaldehyde (HCHO) in anhydrous methanol (CH3OH) as a case study to quantify HCHO in non-aqueous samples. At higher concentrations (C > 0.07 M), we detect a product of HCHO, methoxy methanol (MM, CH3OCH2OH), by Fourier transform infrared spectroscopy, FTIR. Formaldehyde reacts with CH3OH, CD3OH, and CD3OD as shown by FTIR with a characteristic spectral feature around 1,195 cm−1 for CH3OH used for the qualitative detection of MM, a formaldehyde derivative in neat methanol. Ab initio calculations support this assignment. The extinction coefficient for 1,195 cm−1 is in the order of 1.4 × 102 M−1cm−1, which makes the detection limit by FTIR in the order of 0.07 M. For lower concentrations, we performed the quantitative analysis of non-aqueous samples by derivatization with dinitrophenylhydrazine (DNPH). The derivatization uses an aqueous H2SO4 solution to yield the formaldehyde derivatized hydrazone. Ba(OH)2 removes sulfate ions from the derivatized samples and a final extraction with isobutyl acetate to yield a 1:1 methanol: isobutyl acetate solvent for injection for electrospray ionization (ESI). The ESI analysis gave a linear calibration curve for concentrations from 10 to 200 µM with a time-of-flight analyzer (TOF). The detection and quantification limits are 7.8 and 26 μM, respectively, for a linear correlation with R2 > 0.99. We propose that the formaldehyde in CH3OH is in equilibrium with the MM species, without evidence of HCHO in solution. In the presence of water, the peaks for MM become less resolved, as expected from the well-known equilibria of HCHO that favors the formation of methylene glycol and polymeric species. Our results show that HCHO, in methanol does not exist in the aldehyde form as the main chemical species. Still, HCHO is in equilibrium between the production of MM and the formation of hydrated species in the presence of water. We demonstrate the ESI-MS analysis of HCHO from a non-aqueous TiO2 suspension in methanol. Detection of HCHO after illumination of the colloid indicates that methanol photooxidation yields formaldehyde in equilibrium with the solvent.
A 3D Printer Guide for the Development and Application of Electrochemical Cells and Devices
Authors: Silva Ana Luisa , Salvador Gabriel Maia da Silva , Castro Sílvia V. F. , Carvalho Nakédia M. F. , Munoz Rodrigo A. A.
Published: July 2021

3D printing is a type of additive manufacturing (AM), a technology that is on the rise and works by building parts in three dimensions by the deposit of raw material layer upon layer. In this review, we explore the use of 3D printers to prototype electrochemical cells and devices for various applications within chemistry. Recent publications reporting the use of Fused Deposition Modelling (fused deposition modeling®) technique will be mostly covered, besides papers about the application of other different types of 3D printing, highlighting the advances in the technology for promising applications in the near future. Different from the previous reviews in the area that focused on 3D printing for electrochemical applications, this review also aims to disseminate the benefits of using 3D printers for research at different levels as well as to guide researchers who want to start using this technology in their research laboratories. Moreover, we show the different designs already explored by different research groups illustrating the myriad of possibilities enabled by 3D printing.
Rhythm-Speech Correlations in a Corpus of Senegalese Drum Language
Authors: Ros Sofiya
Published: July 2021

In some African cultures, drumming is used for expressing linguistic meanings. Our research focuses on Senegalese musical traditions of encoding linguistic messages on the sabar drums. Senegalese drummers have the practice of playing drums in correlation to speech. We consider rhythms and their linguistic correlates as being part of a Sabar drum language. The long-term goal of this investigation is to establish the linguistic properties of the Sabar drum language. To this end, this work relies on two kinds of research materials collected from Senegalese drummers: bàkks (classical sabar phrases, not improvised on the spot) and sabar improvisations including their translation to Wolof. We study the regularities between Wolof units and sabar rhythms in the collected data. We tested the hypothesis of a syllable-level correspondence between Sabar and Wolof, assuming that each sabar stroke represents a syllable or a number of syllables in Wolof, where the nature of the correspondence depends on the phonetic or phonological properties of a vowel in a syllable. The analysis has shown that different drum strokes are more commonly associated with different types of vowels (front, central or back; open, mid-open/mid-closed or closed vowels).
COVID-19 and the Digitalisation of Cardiovascular Training and Education—A Review of Guiding Themes for Equitable and Effective Post-graduate Telelearning
Authors: Chong Jun Hua , Chahal C. Anwar A. , Gupta Ajay , Ricci Fabrizio , Westwood Mark , Pugliese Francesca , Petersen Steffen E. , Khanji Mohammed Y.
Published: July 2021

The coronavirus disease-2019 (COVID-19) pandemic has had an unprecedented impact leading to novel adaptations in post-graduate medical education for cardiovascular and general internal medicine. Whilst the results of initial community COVID-19 vaccination are awaited, continuation of multimodality teaching and training that incorporates telelearning will have enduring benefit to post-graduate education and will place educational establishments in good stead to nimbly respond in future pandemic-related public health emergencies. With the rise in innovative virtual learning solutions, medical educators will have to leverage technology to develop electronic educational materials and virtual courses that facilitate adult learning. Technology-enabled virtual learning is thus a timely progression of hybrid classroom initiatives that are already adopted to varying degrees, with a need for faculty to serve as subject matter experts, to host and moderate online discussions, and to provide feedback and overall mentorship. As an extension from existing efforts, simulation-based teaching (SBT) and learning and the use of mixed reality technology should also form a greater core in the cardiovascular medicine curriculum. We highlight five foundational themes for building a successful e-learning model in cardiovascular and general post-graduate medical training: (1) digital solutions and associated infrastructure; (2) equity in access; (3) participant engagement; (4) diversity and inclusion; and (5) patient confidentiality and governance framework. With digitalisation impacting our everyday lives and now how we teach and train in medicine, these five guiding principles provide a cognitive scaffold for careful consideration of the required ecosystem in which cardiovascular and general post-graduate medical education can effectively operate. With due consideration of various e-learning options and associated infrastructure needs; and adoption of strategies for participant engagement under sound and just governance, virtual training in medicine can be effective, inclusive and equitable through the COVID-19 era and beyond.
Analysis of Hub Genes and the Mechanism of Immune Infiltration in Stanford Type a Aortic Dissection
Authors: Gao Haoyu , Sun Xiaogang , Liu Yanxiang , Liang Shenghua , Zhang Bowen , Wang Luchen , Ren Jie
Published: July 2021

Background: Stanford type A aortic dissection (AAD) is a catastrophic disease. An immune infiltrate has been found within the aortic wall of dissected aortic specimens. The recall and activation of macrophages are key events in the early phases of AAD. Herein, the immune filtration profile of AAD was uncovered.

Methods: Gene expression data from the GSE52093, GSE98770 and GSE153434 datasets were downloaded from the Gene Expression Omnibus (GEO). The differentially expressed genes (DEGs) of each dataset were calculated and then integrated. A protein-protein interaction (PPI) network was established with the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING), and the hub genes were identified in Cytoscape. Furthermore, gene ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of hub genes were performed. Finally, we set GSE52093 and GSE98770 as the training set and GSE153434 as the validation set to assess immune infiltration in AAD using CIBERSORTx and analyzed the correlations between immune cells and hub genes in both the training and validation sets.

Results: Sixty-one integrated DEGs were identified. The top 10 hub genes were selected from the PPI network, and 140 biological process (BP) terms and 12 pathways were enriched among the top 10 hub genes. The proportions of monocytes and macrophages were significantly higher in AAD tissues than in normal tissues. Notably, this result was consistent in the training set and the validation set. In addition, we found that among the hub genes, CA9, CXCL5, GDF15, VEGFA, CCL20, HMOX1, and SPP1 were positively correlated with CD14, a cell marker of monocytes, while CA9, CXCL5, GDF15, and VEGFA were positively correlated with CD68, a cell marker of macrophages in the training set. Finally, according to the results of the GO and KEGG analysis of hub genes, we found that the monocyte/macrophage-related genes were involved in immune-inflammatory responses through degradation of the extracellular matrix, endothelial cell apoptosis, hypoxia and the interaction of cytokines and chemokines.

Conclusion: The monocyte-macrophage system plays a major role in immune-inflammatory responses in the development of AAD. Several hub genes are involved in this process via diverse mechanisms.
Global, Regional, and National Burden of Myocarditis From 1990 to 2017: A Systematic Analysis Based on the Global Burden of Disease Study 2017
Authors: Wang Xiqiang , Bu Xiang , Wei Linyan , Liu Jing , Yang Dandan , Mann Douglas L. , Ma Aiqun , Hayashi Tomohiro
Published: July 2021

Objective: The global trends in myocarditis burden over the past two decades remain poorly understood and might be increasing during the coronavirus disease 2019 (COVID-19) worldwide pandemic. This study aimed to provide comprehensive estimates of the incidence, mortality, and disability-adjusted life years (DALYs) for myocarditis globally from 1990 to 2017.

Methods: Data regarding the incidence, mortality, DALY, and estimated annual percentage change (EAPC) between 1990 and 2017 for myocarditis worldwide were collected and calculated from the 2017 Global Burden of Disease study. We additionally calculated the myocarditis burden distribution based on the Socio-Demographic Index (SDI) quintile and Human Development Index (HDI).

Results: The incidence cases of myocarditis in 2017 was 3,071,000, with a 59.6% increase from 1990, while the age-standardized incidence rate (ASIR) was slightly decreased. The number of deaths due to myocarditis increased gradually from 27,120 in 1990 to 46,490 in 2017. The middle SDI quintile showed the highest number of myocarditis-related deaths. On the contrary, the global age-standardized death rate (ASDR) decreased with an overall EAPC of −1.4 [95% uncertainty interval (UI) = −1.8 to −1.0]. Similar to ASDR, the global age-standardized DALY rate also declined, with an EAPC of −1.50 (95% UI = −2.30 to −0.8) from 1990 to 2017. However, there was a 12.1% increase in the number of DALYs in the past 28 years; the middle SDI and low-middle SDI quintiles contributed the most to the DALY number in 2017. We also observed significant positive correlations between the EPAC of age-standardized rate and HDI for both death and DALY in 2017.

Conclusions: Globally, the ASIR, ASDR, and age-standardized DALY rate of myocarditis decreased slightly from 1990 to 2017. The middle SDI quintile had the highest level of ASIR, ASDR, and age-standardized DALY rate, indicating that targeted control should be developed to reduce the myocarditis burden especially based on the regional socioeconomic status. Our findings also provide a platform for further investigation into the myocarditis burden in the era of COVID-19.
From General Principles to Procedural Values: Responsible Digital Health Meets Public Health Ethics
Authors: Nyrup Rune
Published: July 2021

Most existing work in digital ethics is modeled on the “principlist” approach to medical ethics, seeking to articulate a small set of general principles to guide ethical decision-making. Critics have highlighted several limitations of such principles, including (1) that they mask ethical disagreements between and within stakeholder communities, and (2) that they provide little guidance for how to resolve trade-offs between different values. This paper argues that efforts to develop responsible digital health practices could benefit from paying closer attention to a different branch of medical ethics, namely public health ethics. In particular, I argue that the influential “accountability for reasonableness” (A4R) approach to public health ethics can help overcome some of the limitations of existing digital ethics principles. A4R seeks to resolve trade-offs through decision-procedures designed according to certain shared procedural values. This allows stakeholders to recognize decisions reached through these procedures as legitimate, despite their underlying disagreements. I discuss the prospects for adapting A4R to the context of responsible digital health and suggest questions for further research.
Evaluating the Effectiveness of Medical–Dental Integration to Close Preventive and Disease Management Care Gaps
Authors: Mosen David M. , Banegas Matthew P. , Dickerson John F. , Fellows Jeffrey L. , Pihlstrom Daniel J. , Kershah Hala M. , Scott Jason L. , Keast Erin M.
Published: July 2021

Background: The integration of medical care into the dental setting has been shown to facilitate the closure of care gaps among patients with unmet needs. However, little is known about whether program effectiveness varies depending on whether the care gap is related to preventive care or disease management.

Materials and Methods: We used a matched cohort study design to compare closure of care gaps between patients aged 65+ who received care at a Kaiser Permanente Northwest (KPNW) Medical–Dental Integration (MDI) clinic or a non-MDI dental clinic between June 1, 2018, and December 31, 2019. The KPNW MDI program focuses on closing 12 preventive (e.g., flu vaccines) and 11 disease management care gaps (e.g., HbA1c testing) within the dental setting. Using the multivariable logistic regression, we separately analyzed care gap closure rates (yes vs. no) for patients who were overdue for: (1) preventive services only (n = 1,611), (2) disease management services only (n = 538), or (3) both types of services (n = 429), analyzing closure of each care gap type separately. All data were obtained through the electronic health record of KPNW.

Results: The MDI patients had significantly higher odds of closing preventive care gaps (OR = 1.51, 95% CI = 1.30–1.75) and disease management care gaps (OR = 1.65, 95% CI = 1.27–2.15) than the non-MDI patients when they only had care gaps of one type or the other. However, no significant association was found between MDI and care gap closure when patients were overdue for both care gap types.

Conclusions: Patients with care gaps related to either preventive care or disease management who received dental care in an MDI clinic had higher odds of closing these care gaps, but we found no evidence that MDI was helpful for those with both types of care gaps.

Practical Implications: MDI may be an effective model for facilitating the delivery of preventive and disease management services, mainly when patients are overdue for one type of these services. Future research should examine the impact of MDI on long-term health outcomes.
An Analysis of (Sub-)Hourly Rainfall in Convection-Permitting Climate Simulations Over Southern Sweden From a User’s Perspective
Authors: Olsson Jonas , Du Yiheng , An Dong , Uvo Cintia B. , Sörensen Johanna , Toivonen Erika , Belušić Danijel , Dobler Andreas
Published: July 2021

To date, the assessment of hydrological climate change impacts, not least on pluvial flooding, has been severely limited by i) the insufficient spatial resolution of regional climate models (RCMs) as well as ii) the simplified description of key processes, e.g., convective rainfall generation. Therefore, expectations have been high on the recent generation of high-resolution convection-permitting regional climate models (CPRCMs), to reproduce the small-scale features of observed (extreme) rainfall that are driving small-scale hydrological hazards. Are they living up to these expectations? In this study, we zoom in on southern Sweden and investigate to which extent two climate models, a 3-km resolution CPRCM (HCLIM3) and a 12-km non-convection permitting RCM (HCLIM12), are able to reproduce the rainfall climate with focus on short-duration extremes. We use three types of evaluation–intensity-based, time-based and event-based–which have been designed to provide an added value to users of high-intensity rainfall information, as compared with the ways climate models are generally evaluated. In particular, in the event-based evaluation we explore the prospect of bringing climate model evaluation closer to the user by investigating whether the models are able to reproduce a well-known historical high-intensity rainfall event in the city of Malmö 2014. The results very clearly point at a substantially reduced bias in HCLIM3 as compared with HCLIM12, especially for short-duration extremes, as well as an overall better reproduction of the diurnal cycles. Furthermore, the HCLIM3 model proved able to generate events similar to the one in Malmö 2014. The results imply that CPRCMs offer a clear potential for increased confidence in future projections of small-scale hydrological climate change impacts, which is crucial for climate-proofing, e.g., our cities, as well as climate modeling in general.
The Driving Forces Underlying Spatiotemporal Lake Extent Changes in the Inner Tibetan Plateau During the Holocene
Authors: Liu Xiangjun , Madsen David , Zhang Xiaojian
Published: July 2021

The Inner Tibetan Plateau (ITP), the central and western part of the Tibetan Plateau (TP), covers about one-fourth of the entire TP and contains more than 800 endorheic lakes larger than 1 km2. These lakes are important water reservoirs and sensitive to TP climate changes. They regulate regional water circulations, and further influence local ecosystems. Many lakes in ITP are surrounded by conspicuous paleoshorelines indicating much higher past lake levels. Previous studies found that lakes in the western ITP (west of ∼86°E) apparently expanded to higher levels than those to the east during the Holocene high lake level stage, however, there is no in-depth study on the reasons for the spatial differences of high lake levels within the ITP. In this study, we first identify Holocene lake level (or lake extent) changes over the ITP by combining published lake level variation data with our reconstruction of Dagze Co lake level variations. We then investigate spatial differences in the magnitude of lake expansions and explore the underlying forces driving these differences using the transient climate evolution of the last 21 ka (TraCE-21ka) and Kiel Climate Model (KCM) simulation results. We find that lakes in the ITP expanded to their highest levels during the early Holocene when the Indian summer monsoon (ISM) greatly intensified. After the mid-Holocene, lake levels fell as a result of the weakening of the ISM. The early Holocene northward shift of the westerly jet and a positive phase of the Atlantic multidecadal oscillation (AMO) resulted in the intensification of southwesterly winds on the southwest TP flank. Concurrently, westerly winds over the TP weakened, causing a differential increase in water vapor transport to the ITP with higher precipitation levels in the southwestern ITP and lower levels to the northeast. These wind-driven differential precipitation levels caused lakes in the southwestern ITP to expand to higher levels than those in the central, northern and northeastern ITP. During the early Holocene, expansion of lakes in the northwestern ITP was enhanced by an increase in glacier melt water besides the increased summer rainfall associated with the intensified ISM.
Spectrum of Endocrine Dysfunction and Association With Disease Severity in Patients With COVID-19: Insights From a Cross-Sectional, Observational Study
Authors: Das Liza , Dutta Pinaki , Walia Rama , Mukherjee Soham , Suri Vikas , Puri Goverdhan Dutt , Mahajan Varun , Malhotra Pankaj , Chaudhary Shakun , Gupta Rahul , Jayant Satyam Singh , Agrawal Kanhaiya , Kumar Vijay , Sachdeva Naresh , Rastogi Ashu , Bhadada Sanjay Kumar , Ram Sant , Bhansali Anil
Published: July 2021

Introduction

Evidence on new-onset endocrine dysfunction and identifying whether the degree of this dysfunction is associated with the severity of disease in patients with COVID-19 is scarce.

Patients and Methods

Consecutive patients enrolled at PGIMER Chandigarh were stratified on the basis of disease severity as group I (moderate-to-severe disease including oxygen saturation <94% on room air or those with comorbidities) (n= 35) and group II (mild disease, with oxygen saturation >94% and without comorbidities) (n=49). Hypothalamo-pituitary-adrenal, thyroid, gonadal axes, and lactotroph function were evaluated. Inflammatory and cell-injury markers were also analysed.

Results

Patients in group I had higher prevalence of hypocortisolism (38.5 vs 6.8%, p=0.012), lower ACTH (16.3 vs 32.1pg/ml, p=0.234) and DHEAS (86.29 vs 117.8µg/dl, p= 0.086) as compared to group II. Low T3 syndrome was a universal finding, irrespective of disease severity. Sick euthyroid syndrome (apart from low T3 syndrome) (80.9 vs 73.1%, p= 0.046) and atypical thyroiditis (low T3, high T4, low or normal TSH) (14.3 vs 2.4%, p= 0.046) were more frequent in group I than group II. Male hypogonadism was also more prevalent in group I (75.6% vs 20.6%, p=0.006) than group II, with higher prevalence of both secondary (56.8 vs 15.3%, p=0.006) and primary (18.8 vs 5.3%, p=0.006) hypogonadism. Hyperprolactinemia was observed in 42.4% of patients without significant difference between both groups.

Conclusion

COVID-19 can involve multiple endocrine organs and axes, with a greater prevalence and degree of endocrine dysfunction in those with more severe disease.
Mouse Spexin: (I) NMR Solution Structure, Docking Models for Receptor Binding, and Histological Expression at Tissue Level
Authors: Wong Matthew K. H. , He Mulan , Sze Kong Hung , Huang Tao , Ko Wendy K. W. , Bian Zhao-Xiang , Wong Anderson O. L.
Published: July 2021

Spexin (SPX), a highly conserved neuropeptide, is known to have diverse functions and has been implicated/associated with pathological conditions, including obesity, diabetes, anorexia nervosa, and anxiety/mood disorders. Although most of the studies on SPX involved the mouse model, the solution structure of mouse SPX, structural aspects for SPX binding with its receptors GalR2/3, and its cellular expression/distribution in mouse tissues are largely unknown. Using CD and NMR spectroscopies, the solution structure of mouse SPX was shown to be in the form of a helical peptide with a random coil from Asn1 to Pro4 in the N-terminal followed by an α-helix from Gln5 to Gln14 in the C-terminus. The molecular surface of mouse SPX is largely hydrophobic with Lys11 as the only charged residue in the α-helix. Based on the NMR structure obtained, docking models of SPX binding with mouse GalR2 and GalR3 were constructed by homology modeling and MD simulation. The models deduced reveal that the amino acids in SPX, especially Asn1, Leu8, and Leu10, could interact with specific residues in ECL1&2 and TMD2&7 of GalR2 and GalR3 by H-bonding/hydrophobic interactions, which provides the structural evidence to support the idea that the two receptors can act as the cognate receptors for SPX. For tissue distribution of SPX, RT-PCR based on 28 tissues/organs harvested from the mouse demonstrated that SPX was ubiquitously expressed at the tissue level with notable signals detected in the brain, GI tract, liver, gonad, and adrenal gland. Using immunohistochemical staining, protein signals of SPX could be located in the liver, pancreas, white adipose tissue, muscle, stomach, kidney, spleen, gonad, adrenal, and hypothalamo-pituitary axis in a cell type-specific manner. Our results, as a whole, not only can provide the structural information for ligand/receptor interaction for SPX but also establish the anatomical basis for our on-going studies to examine the physiological functions of SPX in the mouse model.
Metabolic and Hormonal Profile of Adolescent Girls With Polycystic Ovary Syndrome With Concomitant Autoimmune Thyroiditis
Authors: Skrzyńska Karolina Joanna , Zachurzok Agnieszka , Gawlik Aneta Monika
Published: July 2021

Introduction

Both polycystic ovary syndrome (PCOS) and autoimmune thyroiditis (AT) are considered to be among the most common endocrinopathies in young women, and they are classified as diseases that affect many processes in the human body. Their role in the development of metabolic disorders and diseases of the cardiovascular system in adult women is also emphasized. However, there are no data available to assess such risk in the teenage girl population. The aim of the study was to assess the hormonal and metabolic profile of adolescent girls with PCOS, additionally diagnosed with AT, as well as to identify possible risk factors for the coexistence of AT and PCOS.

Material and Methods

80 euthyroidic PCOS patients were qualified for the study (chronological age 16.54 ± 1.00 years, BMI 24.60 ± 4.16 kg/m2). Eighteen girls diagnosed with AT were included in the study group and 62 girls without AT—in the control group. Each patient had biochemical and hormonal tests performed. Additionally, to diagnose AT, the level of antibodies against thyroid peroxidase (anti-TPO) and anti-thyroglobulin (anti-TG), as well as the image of the thyroid gland on ultrasound examination, were taken into account.

Results

Estradiol concentration was significantly higher in the study than in the control group (203.00 ± 217.00 vs. 152.00 ± 78.50 pmol/L, p=0.02). Higher DHEAS concentrations were also observed in the AT group compared with the group without AT (391.28 ± 176.40 vs. 317.93 ± 114.27 µg/dl, p=0.04). Moreover, there was a positive correlation between AT and estradiol concentration (ry=0.27; p=0.04). It was also shown that there is a tendency toward statistical significance for the positive correlation between the positive anti-TPO titer and the glucose concentration at 120 min OGTT (rƴ=0.26; p=0.07) and girls with PCOS and AT had higher glucose levels in 120 min OGTT (115.29±41.70 vs. 98.56±28.02 mg/dl, p=0.08).

Conclusion

The study results showed no difference in the metabolic profile between the groups. The high concentration of estradiol found in girls with PCOS and AT may indicate the role of this hormone in the development of the autoimmune process. However, the numbers are small, and more research is needed to confirm our findings.
Design Optimization and Dynamic Simulation of Steam Cycle Power Plants: A Review
Authors: Martelli Emanuele , Alobaid Falah , Elsido Cristina
Published: July 2021

After more than one century from its first use for electric power production, steam cycles are still the object of continuous research and development efforts worldwide. Indeed, owing to its favorable thermodynamic properties, steam cycles are not only used in coal-fired power plants but in a large variety of applications such as combined cycles, concentrated solar power plants and polygeneration plants. On the other hand, to cope with the efficiency and flexibility requirements set by today’s energy markets, the design and the operation of steam cycles must be carefully optimized. A key rule is played by the simulation and optimization codes developed in the last 30 years. This paper provides an introduction to the main types of simulation and optimization problems (design, off-design operation and dynamic), an overview of the mathematical background (possible solution approaches, numerical methods and available software), and a review of the main scientific contributions.
How Does Target-Based Performance Evaluation Affect the Accuracy of Energy-Saving Data: Evidence From China
Authors: Zhang Ping , Yu Tiantian , Lu Shengfeng , Huang Ruting
Published: July 2021

Energy intensity measured by energy consumption per unit of GDP has always been the main assessment indicator for the design of energy-saving policies, but its accuracy is highly dependent on the reliability of GDP data. This paper finds that the indicator accuracy is improving after the central government has included energy intensity into the performance appraisal system for local officials. This means that the energy-saving target-based performance evaluation has restrained the data misrepresentation behavior of local officials. Further mechanism analysis shows that the pressure of energy saving restricts the development of the industrial sector, which weakens the ability of local governments to manipulate GDP data, thus improving the accuracy of energy intensity statistics. These findings provide some insightful references for China’s future green development and policy design.
Hydrogeological Criteria to Improve the Sponge City Strategy of China
Authors: Jin Mengxiao , Lancia Michele , Tian Yong , Viaroli Stefano , Andrews Charles , Liu Junguo , Zheng Chunmiao
Published: July 2021

China is facing frequent waterlogging and an increasing water scarcity that mirrors the fast urban and economic expansion of the last 4 decades. To mitigate these issues, the government promulgated the “Sponge City” strategy; a concept rooted in practices in western countries aimed at collecting and reusing 65–90% of urban rainfall. The application consists of absorbent infrastructures such as green roofs and rain gardens combined with the pre-existing urban environment. However, due to climate heterogeneities and the different urbanization contexts in China, these goals may seem overly ambitious in many areas of the country. Compact urbanization, together with heavy rainfall concentrated in short events, puts dramatic stresses on these infrastructures. At the same time, overdesigned infrastructures are expensive and may not be practical to retrofit in existing urban areas. In this paper, the role of urban aquifers as natural Sponge City elements are investigated throughout China. The method of implementation is inexpensive and easy to apply, favoring the direct infiltration to the subsoil after the conversion of the urban surfaces from impervious to permeable. Infiltration to urban aquifers alleviates the pressure on sewers, urban streams, as well as waste-water treatment plants. Considering urban aquifers with different hydraulic characteristics, water table dynamics after large infiltration events from rainfall are simulated via numerical analysis. Hydrogeological and geomorphological analyses are carried out to individuate criteria for the mapping of high absorbance areas at the regional and local scales. A Sponge City approach involving the urban aquifers can represent a winning formula for the success of this ambitious but compelling plan.
Cues for Directional Hearing in the Fly Ormia ochracea
Authors: Mason Andrew C.
Published: July 2021

Insects are often small relative to the wavelengths of sounds they need to localize, which presents a fundamental biophysical problem. Understanding novel solutions to this limitation can provide insights for biomimetic technologies. Such an approach has been successful using the fly Ormia ochracea (Diptera: Tachinidae) as a model. O. ochracea is a parasitoid species whose larvae develop as internal parasites within crickets (Gryllidae). In nature, female flies find singing male crickets by phonotaxis, despite severe constraints on directional hearing due to their small size. A physical coupling between the two tympanal membranes allows the flies to obtain information about sound source direction with high accuracy because it generates interaural time-differences (ITD) and interaural level differences (ILD) in tympanal vibrations that are exaggerated relative to the small arrival-time difference at the two ears, that is the only cue available in the sound stimulus. In this study, I demonstrate that pure time-differences in the neural responses to sound stimuli are sufficient for auditory directionality in O. ochracea.