bluuebunny/crossref_metadata_2025 huggingface dataset:
Denovo Production of Resveratrol by Engineered Rice Wine Strain Saccharomyces cerevisiae HJ08 and Its Application in Rice Wine Brewing
Authors: An Huihui , Li Guangpeng , Yang Zhihan , Xiong Meng , Wang Na , Cao Xitao , Yu Aiqun
Published: July 2024

Resveratrol is a plant-derived polyphenolic compound with numerous biological activities and health-promoting properties. Rice wine is a popular traditional alcoholic beverage made from fermented rice grains, and widely consumed in Asia. To develop resveratrol-enriched rice wine, a heterologous resveratrol biosynthesis pathway was established by integrating the 4-coumaroyl-CoA ligase (Pc4CL) and the stilbene synthase (VvSTS) from Petroselinum crispum and Vitis vinifera at the δ locus sites of industrial rice wine strains Saccharomyces cerevisiae HJ. The resulting S. cerevisiae HJ01 produced a level of 0.6 ± 0.01 mg/L resveratrol. Next, the resveratrol production was increased 16.25-fold through employing the fused protein Pc4CL::VvSTS with a rigidly linked peptide (TPTP, EAAAK). Then, the strains were further modified by removing feedback inhibition of tyrosine through point mutation of ARO4 and ARO7, which integrated at the rDNA region of strain HJ03, and generated strain HJ06, HJ07, and HJ08. Subsequently, the highest resveratrol titer (34.22 ± 3.62 mg/L) was obtained by optimizing fermentation time and precursor addition amount. Finally, resveratrol content of rice wine fermented with strain HJ08 was 2.04 ± 0.08 mg/L and 1.45 ± 0.06 mg/L with or without the addition of 400 mg/L tyrosine after 7 days fermentation.
Differences in Person-Centered Care in Fetal Care Centers: Results from the U.S. Pilot Study of the PCC-FCC Scale
Authors: Wilpers Abigail B. , Francis Katie , Powne Amy B. , Somers Lonnie , Ren Yunyi , Kohari Katherine , Lorch Scott A.
Published: July 2024

Objective: We report findings from a U.S. mixed-methods pilot study of the Person-Centered Care in Fetal Care Centers (PCC-FCC) Scale. Methods: Participants, who received care at a U.S. Fetal Care Center (FCC) between 2017 and 2021, completed an online questionnaire providing sociodemographic details, specifics about the care received, qualitative experiences, and scores from the PCC-FCC Scale. Results: Participants’ (n = 247) PCC-FCC scores and qualitative feedback indicate high perceived person-centered care (PCC), particularly in areas of care coordination, respectful care, and patient education. However, 8% scored below the midpoint, and 38% of comments were negative, especially regarding expectation setting, preparation for post-intervention maternal health, and psychosocial support. Public insurance was associated with higher total PCC-FCC (p = 0.03) and Factor 2 scores (p = 0.02) compared to those with private insurance. The qualitative themes trust, clarity, comprehensive care, compassion, and belonging further elucidate the concept of PCC in FCCs. Conclusion: The PCC-FCC Scale pilot study revealed strong overall PCC in FCCs, yet variability in patient experiences suggests areas needing improvement, including expectation setting, preparation for post-intervention maternal health, and psychosocial support. Future research must prioritize diverse samples and continued mixed methodologies to better understand the role of insurance and identify other potential disparities, ensuring comprehensive representation of the FCC patient population.
The Key Role of Nutritional Intervention in Delaying Disease Progression and the Therapeutic Management of Diabetic Kidney Disease—A Challenge for Physicians and Patients
Authors: Peride Ileana , Anastasiu Miruna , Serban Silvia Alexandra , Tiglis Mirela , Ene Razvan , Nechita Ana-Maria , Neagu Tiberiu Paul , Checherita Ionel Alexandru , Niculae Andrei
Published: July 2024

Chronic kidney disease (CKD) represents an increasingly common pathology that affects patients’ quality of life, and it is frequently associated with a high mortality rate, especially in the final stages of the disease. At the same time, diabetes mellitus is a chronic disease that contributes to the increased number of patients with CKD through diabetic kidney disease (DKD). The alternation of hypoglycemia with hyperglycemia is a condition in the occurrence of microvascular complications of diabetes, including DKD, which involves structural and functional changes in the kidneys. The therapeutic management of diabetic nephropathy is a much-discussed topic, both from nutritional medical recommendations and a pharmacotherapy perspective. The diet starting point for patients with DKD is represented by a personalized and correct adjustment of macro- and micronutrients. The importance of nutritional status in DKD patients is given by the fact that it represents a modifiable factor, which contributes to the evolution and prognosis of the disease. Since, in most cases, it is necessary to restrict many types of food, malnutrition must be considered and avoided as much as possible.
Does Managerial Overconfidence Change with Market Conditions? Risk Management for Financial Institutions
Authors: Voon Jan P. , Yeung Wai Lan Victoria , Chan Sze Nam
Published: July 2024

Overconfidence (hubris or overestimation of one’s ability to perform) has been viewed in the finance literature as a character trait that is stable over time, e.g., assuming that if a manager is overconfident, he/she is overconfident all the time. In this paper, we aim to show that managerial overconfidence can be state-contingent, i.e., the level of managerial overconfidence could be influenced by an external economic shock such as the global financial crisis in 2008. A novelty of this paper is to provide evidence for and application of the concept of state-based managerial overconfidence, which is new in the finance literature. Two empirical studies were reported. In the first study (Study 1), we analyzed real market data by linear regression. We found that managerial overconfidence could vary according to changes in the state of the macroeconomy or tightening of corporate governance policies. In the second study (Study 2), we conducted a lab experiment simulating how external manipulations could alter participants’ confidence level. Both our empirical studies provide strong evidence of state-contingent overconfidence by Student’s t-test and contribute to the current finance literature, which assumes overconfidence as a personality trait. Our findings have important practical implications for the credit market. According to the state-contingent overconfidence hypothesis, creditors might reduce the loan amount or the loan duration (or other loan contract terms) too excessively by more than the efficient level during an economic downturn if the offsetting effect of state-contingent overconfidence is ignored.
Exploring the Genetic Roles of Diet and Other Modifiable Risk Factors in the Risk of Angina: A Causal Investigation Using Mendelian Randomization in UK Biobank and FinnGen Cohorts
Authors: Al Ageeli Essam
Published: July 2024

Background: Angina pectoris, a debilitating manifestation of coronary artery disease, has been associated with various modifiable risk factors. However, the causal underpinnings of these associations remain unclear. This study leveraged Mendelian randomization (MR) to investigate the causal roles of dietary patterns, smoking behaviors, body mass index (BMI), and physical activity in the development of angina. Methods: Two-sample MR analyses were performed using summary-level data from large-scale genome-wide association studies (GWASs) and biobank resources, including the UK Biobank (UKB) and FinnGen cohorts. Genetic variants associated with various types of exposure such as fruit and salad intake, smoking initiation and intensity, BMI, and physical activity were used as instrumental variables, and their causal effects on angina risk were assessed. Results: In the UKB cohort (336,683 individuals, 10,618 cases), genetically proxied fruit (OR = 0.95, 95% CI: 0.93–0.97) and cheese intake (OR = 0.98, 95% CI: 0.97–0.99) were associated with decreased angina risk, while smoking initiation (OR = 1.01, 95% CI: 1.002–1.012), maternal smoking (OR = 1.06, 95% CI: 1.03–1.09), and BMI (OR = 1.01, 95% CI: 1.01–1.02) were associated with increased risk. In the FinnGen cohort (206,008 individuals, 18,168 cases), fruit (OR = 0.30, 95% CI: 0.17–0.53) and salad intake (OR = 0.31, 95% CI: 0.12–0.55) were found to be protective, while smoking initiation (OR = 1.20, 95% CI: 1.04–1.37) and intensity (OR = 1.15, 95% CI: 1.04–1.26) and BMI (OR = 1.31, 95% CI: 1.18–1.47) increased angina risk. Conclusions: This study provides robust evidence for the causal roles of various modifiable risk factors associated with angina development, highlighting the potential benefits of dietary interventions that promote increased fruit and vegetable consumption, smoking cessation, and weight management to mitigate angina risk. Further investigation is needed to generalize these findings to populations with diverse genetic backgrounds, lifestyles, and environmental exposures.
A Comparison of the Effects of Continuous Illumination and Day/Night Regimes on PHB Accumulation in Synechocystis Cells
Authors: Fleischhacker-Daffert Christina , Zerobin Antonia , Hummel Ferdinand , Slaninova Eva , Kroupová Zuzana , Obruca Stanislav , Mrazova Katerina , Hrubanova Kamila , Krzyzanek Vladislav , Nebesarova Jana , Ludwig Katharina , Fritz Ines
Published: July 2024

Poly(3-hydroxybutyrate) (PHB) is a biobased and biodegradable polymer with properties comparable to polypropylene and therefore has the potential to replace conventional plastics. PHB is intracellularly accumulated by prokaryotic organisms. For the cells PHB functions manly as carbon and energy source, but all possible functions of PHB are still not known. Synechocystis (cyanobacteria) accumulates PHB using light as energy and CO2 as carbon source. The main trigger for PHB accumulation in cyanobacteria is nitrogen and phosphorous depletion with simultaneous surplus of carbon and energy. For the above reasons, obtaining knowledge about external factors influencing PHB accumulation is of highest interest. This study compares the effect of continuous light exposure and day/night (16/8 h) cycles on selected physiology parameters of three Synechocystis strains. We show that continuous illumination at moderate light intensities leads to an increased PHB accumulation in Synechocystis salina CCALA 192 (max. 14.2% CDW – cell dry weight) compared to day/night cycles (3.7% CDW). In addition to PHB content, glycogen and cell size increased, while cell density and cell viability decreased. The results offer new approaches for further studies to gain deeper insights into the role of PHB in cyanobacteria to obtain bioplastics in a more sustainable and environmentally friendly way.
Evaluation of the Impact of Chemical Mutagens on the Phenological and Biochemical Characteristics of Two Varieties of Soybean (Glycine max L.)
Authors: Hamisu Anas , Koul Bhupendra , Arukha Ananta Prasad , Al Nadhari Saleh , Rabbee Muhammad Fazle
Published: July 2024

Mutagenic effectiveness and efficiency are the most important factors determining the success of mutation breeding, a coherent tool for quickly enhancing diversity in crops. This study was carried out at Lovely Professional University’s agricultural research farm in Punjab, India, during the year 2023. The experimental design followed a randomized complete block design (RCBD) with three replications. The experiment aimed to assess the effect of three chemical mutagens, sodium azide (SA), ethyl methyl sulphonates (EMSs), and methyl methane sulfonate (MMS), at three different concentrations (0.2%, 0.4%, and 0.6%), in SL958 and SL744 soybean varieties to select the mutant exhibiting the highest yield. The data were collected and analysed using a two-way ANOVA test through SPSS software (version 22), and the means were separated using Duncan’s multiple range test (DMRT) at the 5% level of significance. Between the two varieties, the highest seed germination percentage (76.0% seedlings/plot) was recorded in SL958 (0.4% SA), while the lowest (30.33% seedlings/plot) was observed in 0.6% MMS as compared to the control (53% and 76% in SL744 and SL958 at 10 days after sowing, respectively). Several weeks after sowing, the average plant height was observed to be higher (37.84 ± 1.32 cm) in SL958 (0.4% SA) and lower (20.58 ± 0.30 cm) in SL744 (0.6% SA), as compared to the controls (SL958: 26.09 ± 0.62 cm and SL744: 27.48 ± 0.74 cm). The average leaf count was the highest (234.33 ± 3.09 tetrafoliate leaves/plant) in SL958 (0.4% SA) while it was the lowest (87 leaves/plant) in 0.6% MMS as compared to the control (SL744 180.00 ± 1.63 and SL958 160.73 ± 1.05). The highest total leaf areas recorded in the SL958 and SL744 M1plants were 3625.8 ± 1.43 cm2 and 2311.03 ± 3.65 cm2, respectively. Seeds of the SL958 variety treated with 0.4% SA resulted in the development of tetrafoliate leaves with a broad leaf base and the maximum yield (277.55 ± 1.37 pods/plant) compared to the narrow pentafoliate leaves obtained through the treatment with EMS. Meanwhile, in the SL744 variety, the same treatment led to tetrafoliate leaves with a comparatively lower yield of 206.54 ± 23.47 pods/plant as compared to the control (SL744 164.33 ± 8.58 and SL958 229.86 ± 0.96). The highest protein content (47.04 ± 0.87% TSP) was recorded in the SL958 (0.4% SA) M2 seeds followed by a content of 46.14 ± 0.64% TSP in the SL744 (0.4% SA) M2 seeds, whereas the lowest content (38.13 ± 0.81% TSP) was found in SL958 (0.6% MMS). Similar observations were recorded for the lipid and fibre content. The 0.4% SA treatment in SL958 proved to be efficient in generating the highest leaf area (tetrafoliate leaves) and a reasonable yield of M1 (the first generation after mutation) plants.
Sensitization to Lanolin in North-Eastern Italy, 1997–2021: Prevalence, Risk Factors and the Impact of Occupation
Authors: Cegolon Luca , Larese Filon Francesca ,   on behalf of the North-East Research Group on Contact Dermatitis
Published: July 2024

Background: Direct skin contact with items containing lanolin can induce sensitization and development of contact dermatitis (CD). This multi-centric study investigated prevalence of lanolin sensitization among 30,269 outpatients from North-Eastern Italy patch tested during 1997–2021. Methods: European baseline and extended Triveneto series were applied on the upper part of patients’ back and removed after 48 h. Risk factors for lanolin sensitization were investigated by multiple logistic regression analysis, reporting adjusted odds ratios (aOR) with 95% confidence interval (95%CI). Results. Overall lanolin patch test positive ratio (PTPR) was 1.64% (=501/30,629), with variability over time and by research center. The body area most frequently affected by CD were hands (36.32%), followed by face (19.52%) and legs (8.09%), with a lanolin PTPR of 1.68%, 1.37% and 3.07%, respectively. Prevalence of occupational CD was 8.24%, and 1.83% patients with occupational CD patch tested positive against lanolin. Lanolin sensitization was significantly higher in males (aOR = 1.34; 95%CI: 1.08; 1.65) and among patients with leg CD aged 49–60 years (aOR = 2.34; 95%CI: 1.20; 4.57) or older than 60 (aOR = 4.21; 95%CI: 2.59; 6.85). Sub-group analysis confirmed the significantly higher sensitization rate of older patients with leg CD, with much stronger effect size in females 61+ years old (aOR = 5.33; 95%CI 2.87; 9.89) than males in the same age group (aOR = 2.92; 95%CI: 1.34; 6.39). Moreover, female house painters were more likely to test positive to lanolin. Conclusions: The variability of lanolin PTPR over time and by research center endorsed the ongoing debate on the relevance of the respective skin reaction. Clinicians assessing patients with dermatitis should collect information on potential risk factors for lanolin sensitization, particularly use of skin care products containing the hapten. Occupational exposure to lanolin-containing varnishes should also be considered.
Multi-Objective Technology-Based Approach to Home Healthcare Routing Problem Considering Sustainability Aspects
Authors: Zaid Ahmed Adnan , Asaad Ahmed R. , Othman Mohammed , Haj Mohammad Ahmad
Published: July 2024

Background: This research aims to solve a home healthcare vehicle routing problem (HHCVRP) model that considers the social aspect of sustainability and will be implemented in smart cities. In addition to the dynamism and uncertainty caused by variations in the patient’s condition, the proposed model considers parameters and variables that enhance its practicability, such as assuming different levels of patient importance (priority). Methods: The model was solved using a metaheuristic algorithm approach via the Ant Colony Optimization algorithm and the Non-Dominated Sorting technique due to the ability of such a combination to work out with dynamic models with uncertainties and multi-objectives. Results: This study proposes a novel mathematical model by integrating body sensors on patients to keep updating their conditions and prioritizing critical conditions in service. The sensitivity analysis demonstrates that using a heart rate sensor improves service quality and patient satisfaction without affecting the energy consumed. In addition, quality costs are increased if the importance levels of patients increase. Conclusions: The suggested model can assist healthcare practitioners in tracking patients’ health conditions to improve the quality of service and manage workload effectively. A trade-off between patient satisfaction and service provider satisfaction should be maintained.
Simulations of Femtosecond-Laser Near-Field Ablation Using Nanosphere under Dynamic Excitation
Authors: Sun Jiaxin , Jiang Lan , Guan Mingle , Liu Jiangfeng , Wang Sumei , Zhu Weihua
Published: July 2024

Femtosecond lasers have garnered widespread attention owing to their subdiffraction processing capabilities. However, their intricate natures, involving intrapulse feedbacks between transient material excitation and laser propagation, often present significant challenges for near-field ablation predictions and simulations. To address these challenges, the current study introduces an improved finite-difference time-domain method (FDTD)–plasma model (plasma)–two-temperature model (TTM) framework for simulating the ablation processes of various nanospheres on diverse substrates, particularly in scenarios wherein dynamic and heterogeneous excitations significantly influence optical-field distributions. Initially, FDTD simulations of a single Au nanosphere on a Si substrate reveal that, with transitions in the excitation states of the substrate, the field-intensity distribution transforms from a profile with a single central peak to a bimodal structure, consistent with experimental reports. Subsequently, simulations of a polystyrene nanosphere array on a SiO2 substrate reveal that different excitation states of the nanospheres yield two distinct modes, namely near-field enhancement and masking. These modes cannot be adequately modeled in the FDTD simulations. Our combined model also considers the intrapulse feedback between the electromagnetic-field distribution resulting from near-field effects and material excitations. Furthermore, the model can quantitatively analyze subsequent electron–phonon coupling and material removal processes resulting from thermal-phase transitions. Consequently, our model facilitates predictions of the femtosecond-laser ablation of single nanospheres or nanosphere arrays with varying sizes and materials placed on substrates subjected to near-field effects.
Characterization of Iron Oxide Nanotubes Obtained by Anodic Oxidation for Biomedical Applications—In Vitro Studies
Authors: Rangel Rita de Cássia Reis , Rangel André Luiz Reis , da Silva Kerolene Barboza , Escada Ana Lúcia do Amaral , Chaves Javier Andres Munoz , Maia Fátima Raquel , Pina Sandra , Reis Rui L. , Oliveira Joaquim M. , Rosifini Alves Ana Paula
Published: July 2024

To improve the biocompatibility and bioactivity of biodegradable iron-based materials, nanostructured surfaces formed by metal oxides offer a promising strategy for surface functionalization. To explore this potential, iron oxide nanotubes were synthesized on pure iron (Fe) using an anodic oxidation process (50 V–30 min, using an ethylene glycol solution containing 0.3% NH4F and 3% H2O, at a speed of 100 rpm). A nanotube layer composed mainly of α-Fe2O3 with diameters between 60 and 70 nm was obtained. The effect of the Fe-oxide nanotube layer on cell viability and morphology was evaluated by in vitro studies using a human osteosarcoma cell line (SaOs-2 cells). The results showed that the presence of this layer did not harm the viability or morphology of the cells. Furthermore, cells cultured on anodized surfaces showed higher metabolic activity than those on non-anodized surfaces. This research suggests that growing a layer of Fe oxide nanotubes on pure Fe is a promising method for functionalizing and improving the cytocompatibility of iron substrates. This opens up new opportunities for biomedical applications, including the development of cardiovascular stents or osteosynthesis implants.
Effect of Packaging Method and Storage Environment on Activity of Magnesium Oxide and Mechanical Properties of Basic Magnesium Sulfate Cement
Authors: Wu Yuxiao , Shi Peini , Yang Qingsong , Zhang Na
Published: July 2024

As one of the raw materials of basic magnesium sulfate cement (BMSC), the activity of light-burned magnesium oxide (MgO) has an important effect on the hydration rate, hydration products, and mechanical properties of BMSC. To reveal the influence of packaging method, storage environment, and storage time on the activity of MgO and the mechanical properties of BMSC, an experiment was conducted by using ordinary woven bags, peritoneal woven bags, and plastic and paper compound bags to store the finished BMSC and the raw materials (light-burned MgO, MgSO4·7H2O, fly ash, and a chemical additive) under the conditions of natural environment, sealed environment, and wet environment, respectively. Comparative analysis of the effects of packaging method, storage conditions, and storage time on the activity of MgO and the mechanical properties of BMSC was performed through the mechanical strength test of mortar specimens. The results showed that in a sealed environment, the loss of a-MgO content in light-burned MgO was minimized, which was more conducive to keeping the mechanical properties of BMSC stable. In the wet environment, the mechanical strength of BMSC was significantly reduced in the early stage (1 day) due to the significant reduction in the activity of MgO, and the mechanical strength of the finished BMSC and prepared BMSC after 120 days of storage was still lost, regardless of the packaging method. However, the storage environment and packaging method had relatively little effect on the late mechanical strength (28 days) of BMSC. It is advisable to use ordinary woven bags for packaging in natural and sealed environments as this is more economical for engineering applications. Plastic and paper compound bags are superior to ordinary woven bags and peritoneal woven bags in wet environments.
Mathematical Modeling of NaCl Scaling Development in Long-Distance Membrane Distillation for Improved Scaling Control
Authors: Cai Jingcheng , Mu Xingsen , Xue Jian , Chen Jiaming , Liu Zeman , Guo Fei
Published: July 2024

Membrane distillation is a novel membrane-based separation technology with the potential to produce pure water from high-salinity brine. It couples transport behaviors along the membrane and across the membrane. The brine in the feed is gradually concentrated due to the permeate flux across the membrane, which is a significant factor in initiating the scaling behavior on the membrane surface along the feed flow direction. It is of great interest to investigate and estimate the development of scaling on the membrane surface. This work specifically focuses on a long-distance membrane distillation process with a sodium chloride solution as the feed. A modeling approach has been developed to estimate the sodium chloride scaling development on the membrane surface along the flow direction. A set of experiments was conducted to validate the results. Based on mathematical simplification and analytical fitting, a simplified model was summarized to predict the initiating position of sodium chloride scaling on the membrane, which is meaningful for scaling control in industrial-scale applications of membrane distillation.
Impact of Juglone, a PIN1 İnhibitor, on Oral Carcinogenesis Induced by 4-Nitroquinoline-1-Oxide (4NQO) in Rat Model
Authors: Topal Olgun , Topal Burcu Güçyetmez , Baş Yunus , Ongan Bünyamin , Sadi Gökhan , Aslan Esra , Yavaş Betül Demirciler , Pektaş Mehmet Bilgehan
Published: July 2024

Background and Objectives: PIN1 is overexpressed in several human cancers, including prostate cancer, breast cancer, and oral squamous carcinomas. Juglone (J), derived from walnut, was reported to selectively inhibit PIN1 by modifying its sulfhydryl groups. In this study, the potential effects of juglone, also known as PIN1 inhibitor, on oral cancer and carcinogenesis were investigated at the molecular level. Materials and Methods: 4-Nitroquinoline N-oxide (4-NQO) was used to create an oral cancer model in animals. Wistar rats were divided into five groups: Control, NQO, Juglone, NQO+J, and NQO+J*. The control group received the basal diet and tap water throughout the experiment. The NQO group received 4-NQO for 8 weeks in drinking water only. The Juglone group was administered intraperitoneally in a juglone solution for 10 weeks (1 mg/kg/day). The NQO+J group received 4-NQO in drinking water for 8 weeks, starting 1 week after the cessation of 4-NQO treatment. They were then administered intraperitoneally in a juglone solution for 10 weeks. (1 mg/kg/day). NQO+J* group: received 4 NQO for 8 weeks in drinking water and administered intraperitoneally in a juglone solution for 10 weeks (1 mg/kg/day). They were sacrificed at the end of the 22-week experimental period. The tongue tissues of the rats were isolated after the experiment, morphological changes were investigated by histological examinations, and the molecular apoptotic process was investigated by rt-qPCR and western blot. Results: Histological results indicate that tumors are formed in the tongue tissue with 4-NQO, and juglone treatment largely corrects the epithelial changes that developed with 4-NQO. It has been determined that apoptotic factors p53, Bax, and caspases are induced by the effect of juglone, while antiapoptotic factors such as Bcl-2 are suppressed. However, it was observed that the positive effects were more pronounced in rats given juglone together with 4-NQO. Conclusions: The use of PIN1 inhibitors such as juglone in place of existing therapeutic approaches might be a promising and novel approach to the preservation and treatment of oral cancer and carcinogenesis. However, further research is required to investigate the practical application of such inhibitors.
Assessment of the Thermomechanical Behavior and Microstructure of AA 7075-T6 Aluminum Alloy Lap Joints at Optimal Predicted FSW Process Parameters
Authors: Toumi Oumayma , Khalifa Romdhane Ben , Silvestri Alessia Teresa , Ennetta Ridha , Scherillo Fabio , Prisco Umberto
Published: July 2024

The lap joints of AA 7075-T6 aluminum alloy were assembled using the friction stir welding (FSW) technique. Experimental studies were performed to characterize the thermomechanical properties of these welds. The main goal of this research was to comprehensively assess the thermomechanical behavior of AA 7075-T6 aluminum alloy under FSW conditions. Tests were carried out at a tool rotational speed of 1320 rpm and at two advancing speeds of 70 mm/min and 120 mm/min, selected based on a previous study aiming to optimize the heat input during the FSW process. The experimental investigations involved the characterization of temperature profiles during welding, mechanical properties such as microhardness and tensile strength, and microstructure examination at the two advancing speed conditions. This study revealed that the welding speed has an obvious influence on the material thermal behavior during the FSW process. Indeed, the peak temperature obtained with a lower welding speed (70 mm/min) was higher by almost 10% compared to that obtained with a higher speed (120 mm/min). Moreover, by increasing the welding speed, the mechanical characteristics, such as microhardness and tensile strength, were increased by almost 5% for the mean microhardness and 6% for the ultimate tensile strength. Additionally, the microstructure examination demonstrated that, by decreasing the welding speed, more interaction between the tool and the material is observed, resulting in a deeper stir zone due to increased heat dissipation downwards into the material, affecting the thermal profile and influencing the resulting mechanical properties of the welded joint.
The Influence of Laser Remelting Parameters on the Surface Quality of Copper
Authors: Li Hengzheng , Chen Yang , Chen Shuai , Liu Yan , Liu Conghu
Published: July 2024

In order to improve the surface quality of copper after laser remelting, this article took laser frequency, pulse width, and energy density as the research objects and used scanning electron microscopy (SEM), a laser confocal three-dimensional measurement instrument, hardness tester, and friction and wear measurement instrument to study the surface morphology, surface roughness, microhardness, and wear resistance of copper, respectively. The results indicate that the frequency, pulse width, and energy density of laser remelting could directly affect the surface quality of the sample, but the influence of frequency and pulse width was more significant. When the laser remelting frequency was 10 Hz, the pulse width was 10 ms, and the energy density was 132.69 J/mm2, the sample exhibited good surface morphology, roughness, and wear resistance. The relevant research in this article can provide a good reference for the laser surface treatment of copper-based materials.
Sensitive and Accurate Quantification of Enterovirus-D68 (EV-D68) Viral Loads Using Droplet Digital PCR (ddPCR)
Authors: Grizer Cassandra S. , Li Zhaozhang , Mattapallil Joseph J.
Published: July 2024

Enterovirus-D68 (EV-D68) is a reemerging virus that has been associated with numerous outbreaks in children in the past 10 years. Most assays examining viral infection kinetics have relied on the use of quantitative RT-PCR (qRT-PCR) assays as an assay of choice. Though valuable, there are inherent limitations that introduce variability, thereby reducing its value when comparing results across the field. Unlike the qRT-PCR assay that uses a standard curve to determine the copy number of viral RNA, the droplet digital PCR assay (ddPCR) directly quantifies the absolute number of copies within a given sample, which in turn makes the assay highly sensitive and accurate. Here, we have developed an EV-D68-specific ddPCR assay that effectively quantifies EV-D68 RNA copies in both cells and supernatants within a dynamic range of 6.7 × 10−3 copies/μL to 1.2 × 104 copies/μL of the sample. The assay was highly specific for a broad range of EV-D68 isolates (Fermon, US/MO/14-18947, US/MO/14-18949, US/KY/14-18953, USA/2018-23088, USA/2020-23336 and EV-D68-infected human nasal turbinate samples from the 2022 outbreak) without cross-reactivity to other viruses such as Enterovirus-A71 (EV-A71), Human Parechovirus (HPeV)-1 and -2, Coxsackievirus (CV)-B1, Human Coronavirus (HCoV)-NL63, SARS-CoV-2, Influenza-A and B, Rhinovirus, and Respiratory Syncytial Virus (RSV)-A2, which are known to cause infection in children. The assay was able to readily quantify EV-D68 in infected cells and supernatants along with nasal turbinate samples collected from children during the 2022 outbreak. Our results suggest that the assay can be readily translated to accurately quantify viral loads in tissues and body fluids such as plasma and lung or nasal aspirates.
Rapid and Highly Sensitive Detection of Mycobacterium tuberculosis Utilizing the Recombinase Aided Amplification-Based CRISPR-Cas13a System
Authors: Li Qiao , Wang Nenhan , Pang Mengdi , Miao Honghao , Dai Xiaowei , Li Bo , Yang Xinyu , Li Chuanyou , Liu Yi
Published: July 2024

Tuberculosis (TB), a disease caused by Mycobacterium tuberculosis (MTB) infection, remains a major threat to global public health. To facilitate early TB diagnosis, an IS6110 gene-based recombinase aided amplification (RAA) assay was coupled to a clustered, regularly interspaced short palindromic repeats (CRISPR)-Cas13a fluorescence assay to create a rapid MTB detection assay (named RAA-CRISPR-MTB). Its diagnostic efficacy was evaluated for sensitivity and specificity through sequential testing of recombinant plasmids, mycobacterium strains, and clinical specimens. RAA-CRISPR detected IS6110 genes at levels approaching 1 copy/μL with pUC57-6110 as the template and 10 copies/μL with H37Rv as the template. There was no observed cross detection of non-tuberculosis mycobacteria (NTM) with either template. Furthermore, RAA-CRISPR testing of 151 clinical specimens yielded a diagnostic specificity rate of 100% and a diagnostic sensitivity rate of 69% that exceeded the corresponding Xpert MTB/RIF assay rate (60%). In conclusion, we established a novel RAA-CRISPR assay that achieved highly sensitive and specific MTB detection for use as a clinical TB diagnostic tool in resource-poor settings.
Investigation and Analysis of Wettability, Anisotropy, and Adhesion in Bionic Upper and Lower Surfaces Inspired by Indocalamus Leaves
Authors: Wang Bo , Chen Donghui , Yang Xiao , Li Ming
Published: July 2024

Nature provides us with a wealth of inspiration for the design of bionic functional surfaces. Numerous types of plant leaves with exceptional wettability, anisotropy, and adhesion are extensively employed in many engineering applications. Inspired by the wettability, anisotropy, and adhesion of indocalamus leaves, bionic upper and lower surfaces (BUSs and BLSs) of the indocalamus leaf were successfully prepared using a facile approach combining laser scanning and chemical modification. The results demonstrated the BUSs and BLSs obtained similar structural features to the upper and lower surfaces of the indocalamus leaf and exhibited enhanced and more-controllable wettability, anisotropy, and adhesion. More importantly, we conducted a detailed comparative analysis of the wettability, anisotropy, and adhesion between BUSs and BLSs. Finally, BUSs and BLSs were also explored for the corresponding potential applications, including self-cleaning, liquid manipulation, and fog collection, thereby broadening their practical utility. We believe that this study can contribute to the enrichment of the research on novel biological models and provide significant insights into the development of multifunctional bionic surfaces.
Greater Post-Operative Nutrition Risks Identified in Pediatric and Adolescent Patients after Anterior Cruciate Ligament Reconstruction Regardless of Age and Sex
Authors: McGinley James J. , Dabis Jessica , Morrison Taylor , Podvin Caroline , Ellis Henry B. , Ulman Sophia
Published: July 2024

Systematic detection of risky nutrition behaviors after sports surgery may better promote healing for return-to-sport. The purpose of this study was to assess nutritional behavior differences between patients following anterior cruciate ligament reconstruction (ACLR) and following other lower-extremity orthopedic surgeries. One pediatric sports medicine center was reviewed for a custom Sports Nutrition Assessment for Consultation, which investigates nutrition-related risk factors for youth athletes at their first post-operative visit. Patients reported “Yes” or “No” to eight questions, after which they were offered a nutrition consultation for any response indicating risk. A total of 243 post-ACLR and 242 non-ACLR patients were reviewed. The post-ACLR patients more often reported a change in appetite (p = 0.021), recent weight changes (p = 0.011), a desire to better understand nutrition (p = 0.004), and recommendations to change their body composition (p = 0.032). More post-ACLR patients were identified for a nutrition consultation (p = 0.002), though an equal percentage accepted the consultation between groups. Age and sex were not determined to be significant confounders after matched sub-analysis. The post-ACLR patients more often reported nutrition risks, specifically weight-related issues, regardless of age or sex. Sports surgeons should regularly inquire about nutrition-related concerns with patients and refer to sports dietitians for recovery nutrition support as needed, particularly after ACLR.
Sourdough Bread with Different Fermentation Times: A Randomized Clinical Trial in Subjects with Metabolic Syndrome
Authors: Pérez-Vega Karla Alejandra , Sanllorente Albert , Zomeño María-Dolores , Quindós Ana , Muñoz-Martínez Júlia , Malcampo Mireia , Aldea-Perona Ana , Hernáez Álvaro , Lluansí Aleix , Llirós Marc , Elias Isidre , Elias-Masiques Núria , Aldeguer Xavier , Muñoz Daniel , Gaixas Sònia , Blanchart Gemma , Schröder Helmut , Hernando-Redondo Javier , Carrón Nerea , González-Torres Pedro , Konstantinidou Valentini , Fitó Montserrat , Castañer Olga
Published: July 2024

The Mediterranean diet, featuring sourdough bread, shows promise in managing metabolic syndrome. This study explored the effects of two sourdough breads, with differing fermentation times but similar nutritional profiles, on inflammation, satiety, and gut microbiota composition in adults with metabolic syndrome. In a double-blind clinical trial, participants were randomized to consume either Elias Boulanger® long-fermentation (48 h) sourdough bread (EBLong) or Elias Boulanger® short-fermentation (2 h) sourdough bread (EBShort) over a two-month period. We assessed clinical parameters, inflammatory biomarkers, satiety-related hormones, and the richness and abundance of gut microbiota at baseline and follow-up. The participants included 31 individuals (mean age, 67, 51.6% female). EBShort was associated with reduced levels of soluble intercellular adhesion molecule (sICAM), and all participants, regardless of the intervention, exhibited a decrease in sICAM and diastolic pressure from baseline (p < 0.017). At follow-up, plasminogen activator inhibitor-1 (PAI-1) levels were lower in EBShort (−744 pg/mL; 95%CI: −282 to −1210 pg/mL) compared to EBLong. No differences in microbiota richness or abundance were observed. EBShort bread was effective in reducing some inflammation markers. The consumption of sourdough bread may offer potential benefits in reducing inflammation markers in individuals with metabolic syndrome; however, longer fermentation times did not show additional benefits.
The Association between the Substitution of Red Meat with Legumes and the Risk of Primary Liver Cancer in the UK Biobank: A Cohort Study
Authors: Bock Niels , Langmann Fie , Johnston Luke , Ibsen Daniel , Dahm Christina
Published: July 2024

Primary liver cancer is globally on the rise, partially due to poor diets and sedentary lifestyles. Shifting to more plant-based diets may lower the risk. We aimed to estimate the effect of replacing total red meat, unprocessed red meat and processed red meat with legumes on primary liver cancer in a free-living population. We analyzed data from 126,744 UK Biobank participants who completed ≥ two 24 h diet recalls. Baseline characteristics were collected from the initial assessment visit. Information on liver cancer diagnoses was collected via external linkage to inpatient hospital episodes or central cancer registries. Cox proportional hazards regression models were used to estimate the substitution of 15 g/day of legumes with 15 g/day of total red meat, unprocessed red meat or processed red meat on liver cancer risk, using the leave-one-out food substitution model. During a median follow-up time of 11.1 years, 173 participants developed liver cancer. In the fully adjusted models, no association was observed when substituting 15 g/day of legumes with total red meat (HR: 1.02 (95% CI 0.96–1.08)), unprocessed red meat (HR: 1.00 (95% CI 0.94–1.06)) or processed red meat (HR: 1.09 (95% CI 0.99–1.21)). Overall, little evidence of an association between replacing red meat with legumes and liver cancer was observed. Further research in other study populations with longer follow-up time is warranted.
Evaluation of the Caffeine Content in Servings of Popular Coffees in Terms of Its Safe Intake—Can We Drink 3–5 Cups of Coffee per Day, as Experts Advise?
Authors: Wierzejska Regina Ewa , Gielecińska Iwona
Published: July 2024

The spreading knowledge of the health benefits of coffee and the development of gastronomy with a wide range of coffees prompt an evaluation of their caffeine content in terms of safe intake. The study analyzed the caffeine content of popular coffees in comparison with recommendations for a safe single dose (200 mg) and daily caffeine intake (400 mg), and guidelines for drinking 3–5 cups of coffee per day. A total of 299 coffee samples from franchise shops and homemade coffees were tested. The “takeaway” coffees had a three times higher mean caffeine content (p < 0.005) compared to homemade coffees. Americano coffee was the “strongest” (143 mg caffeine/serving on average), while coffee prepared by pouring hot water over one teaspoon of ground coffee was the “lightest” (23 mg caffeine/serving on average) (p < 0.05). Over 200 mg of caffeine per serving was found in 4% of samples. Over 400 mg of caffeine would be consumed by people drinking “on the go” 4–5 servings of many types of coffee, except espresso. In this respect, homemade coffees are safer. Therefore, recommendations on drinking coffee should be more practical, and indicate not only the number of cups, but also the “strength” of various types of coffee, in order to avoid the regular intake of high amounts of caffeine.
A Reduction in the Rotational Velocity Measurement Deviation of the Vortex Beam Superposition State for Tilted Object
Authors: Wang Hongyang , Yan Yinyin , Zhang Zijing , Liu Hao , Lv Xinran , Cui Chengshuai , Yun Hao , Feng Rui , Zhao Yuan
Published: July 2024

In measuring object rotational velocity using vortex beam, the incident light on a tilted object causes spectral broadening, which significantly interferes with the identification of the true rotational Doppler shift (RDS) peak. We employed a velocity decomposition method to analyze the relationship between the spectral extremum and the central frequency shift caused by the object tilt. Compared with the linear growth trend observed when calculating the object rotational velocity using the frequency peak with the maximum amplitude, the central frequency calculation method effectively reduced the deviation rate of the RDS and velocity measurement value from the true value, even at large tilt angles. This approach increased the maximum tilt angle for a 1% relative error from 0.221 to 0.287 rad, representing a 29.9% improvement. When the tilt angle was 0.7 rad, the velocity measurement deviation reduction rate can reach 5.85%. Our work provides crucial support for achieving high-precision rotational velocity measurement of tilted object.
Low-Bit-Depth Detection for Phase Retrieval with Higher Efficiency in Holographic Data Storage
Authors: Liu Hongjie , Zheng Shujun , Lin Yongkun , Song Haiyang , Xu Xianmiao , Li Xiong , Zheng Jihong , Cao Qiang , Lin Xiao , Tan Xiaodi
Published: July 2024

In the past, comprehensive information was imperative for image processing, prompting a preference for high-depth cameras. However, in our research, we discovered that the abundance of image details may impede phase retrieval. Consequently, this paper presents an iterative phase retrieval method based on a low bit depth. Through simulations and experiments, this approach has proven effective in evidently enhancing phase retrieval outcomes. Furthermore, the concept of low bit depth holds promise for broader application across diverse domains within the field of image retrieval.
Birefringence and Anisotropy of the Losses Due to Two-Photon Absorption of Femtosecond Pulses in Crystals
Authors: Kovalev Valeri , Krasin George
Published: July 2024

It is shown that the two-photon absorption (TPA)-induced losses in a non-centrosymmetric crystal reduce considerably when the angle θ between the polarization vector of incident radiation and the optical c-axis is 45°. In such a geometry of interaction in a Ca3(VO4)2 crystal, the effective TPA coefficient for 300 fs laser pulses at θ = 45° is 3.5 ± 0.5 times lower than its maximum at θ = 0°, which is more than two times higher than reported earlier in the literature.
Exogenous Melatonin Enhances Cold Tolerance by Regulating the Expression of Photosynthetic Performance, Antioxidant System, and Related Genes in Cotton
Authors: Zhu Jincheng , Lou Hui , Yan Chen , Zhang Wei , Li Zhibo
Published: July 2024

In China, cotton is a significant cash crop, and cold stress negatively impacts the crop’s development, production, and quality formation. Recent studies have shown that melatonin (MT) can alleviate the damage to plants under cold stress and promote good growth and development. In this study, the morphological and physiological changes induced by exogenous melatonin pretreatment on ‘Xinluzao 33’ cotton seedlings under cold stress were examined to investigate its defensive effects. The results showed that 100 μM MT pretreatment improved the cold resistance of cotton most significantly. It also improved the wilting state of cotton under cold stress, greatly increased the photosynthetic rate (Pn), stomatal conductance (Gs), maximum photochemical efficiency (Fv/Fm), and photosynthetic performance index (PIabs) by 116.92%, 47.16%, 32.30%, and 50.22%, respectively, and mitigated the adverse effects of low-temperature. In addition, MT supplementation substantially reduced the accumulation of superoxide anion (O2•−) and hydrogen peroxide (H2O2) by 14.5% and 45.49%, respectively, in cold-stressed cotton leaves by modulating the antioxidant system, thereby mitigating oxidative damage. Furthermore, MT pretreatment increased the endogenous melatonin content (23.80%) and flavonoid content (21.44%) and considerably induced the expression of biosynthesis enzyme-related genes. The above results indicate that exogenous melatonin improves the low-temperature resistance of cotton seedlings by regulating photosynthetic performance, antioxidant enzyme activity, antioxidant content, endogenous melatonin and flavonoid content, and the expression levels of genes related to their synthesis.
Design of High-Performance Molecular Imprinted Magnetic Nanoparticles-Loaded Hydrogels for Adsorption and Photodegradation of Antibiotics from Wastewater
Authors: Curcuruto Giusy , Scamporrino Andrea A. , Puglisi Roberta , Nicotra Giuseppe , Sfuncia Gianfranco , Impellizzeri Giuliana , Dattilo Sandro , Kahru Anne , Sihtmäe Mariliis , Aruoja Villem , Blinova Irina , Carroccio Sabrina Carola
Published: July 2024

A hydrogel formulation of 2-hydroxy ethyl methacrylate (HEMA) containing covalently linked magnetite nanoparticles was developed to actively facilitate the selective removal and photocatalytic degradation of antibiotics. To this purpose, the hybrid materials were molecularly imprinted with Lomefloxacin (Lome) or Ciprofloxacin (Cipro), achieving a selectivity of 60% and 45%, respectively, starting from a solution of XX concentration. After the adsorption, the embedded magnetite was used with the double function of (i) magnetically removing the material from water and (ii) triggering photo-Fenton (PF) reactions assisted by UVA light and H2O2 to oxidize the captured antibiotic. The success of the material design was confirmed by a comprehensive characterization of the system from chemical–physical and morphological perspectives. Adsorption and degradation tests demonstrated the material’s ability to efficiently degrade Lome until its complete disappearance from the electrospray ionization (ESI) mass spectra. Regeneration tests showed the possibility of reusing the material for up to three cycles. Ecotoxicological tests using algae Rapidocelis subcapitata, crustaceans Daphnia magna, and bacteria Vibrio fischeri were performed to evaluate the ecosafety of our synthesized materials.