bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Bi Content on the Microstructure and Mechanical Performance of Sn-1Ag-0.5Cu Solder Alloy
Authors: Zahran Heba Y. , Mahmoud Ashraf S. , Abd El-Rehim Alaa F.
Published: March 2021

The purpose of this work is to explore the impact of 0.5, 1.5, 2.5 and 3.5 wt.% Bi additions on the microstructure and mechanical performance of Sn-1Ag-0.5Cu solder alloy. Scanning electron microscope (SEM) and X-ray diffraction (XRD) were utilized to examine the microstructure of the present solders. Creep measurements have been used for the preliminary assessment of mechanical properties. The steady-state creep rate, έst, diminished as the Bi’s concentration increased and reached 2.5 wt.%, with this trend altering above this point. Furthermore, increasing the aging or testing temperature caused the έst values to increment for all the investigated solders. έst variations with different Bi content and aging temperature were observed by examining the Sn-Ag-Cu solders’ structural evolutions. The mean value of the activation energy of all investigated solder alloys was found to be ∼52 kJ/mol. This value is appropriate to that quoted for the dislocation climb through the core diffusion as the dominant operating mechanism. The XRD findings supported the microstructure and lattice parameters variations with both aging temperatures and bismuth concentrations.
Common Vole as a Focal Small Mammal Species in Orchards of the Northern Zone
Authors: Stirkė Vitalijus , Balčiauskas Linas , Balčiauskienė Laima
Published: March 2021

In 2018–2020, we performed a country-wide study of small mammals in commercial orchards and berry plantations with the aim of determining whether the common vole (Microtus arvalis) is a more suitable focal species than the field vole (M. agrestis) in the risk assessment of plant protection products in Lithuania (country of the Northern Zone). Common vole was present in 75% of orchards and in 80% of control habitats, accounting for 30% of all trapped individuals. The proportion of this species was stable between years and seasons. The pattern was in agreement with the intermediate disturbance hypothesis, i.e., highest in medium-aged crops, while lowest in habitats with high intensities of agricultural practices. The average relative abundance of common vole in autumn, 2.65 ± 0.52 individuals per 100 trap days, was three times higher than that in summer, with no differences recorded between crops and control habitats. Field vole was present in 30% of locations, only accounting for 2.1% of all trapped individuals. In central and eastern European countries, common vole is more widespread and abundant than field vole. In Lithuania, common vole dominates in orchards and natural habitats and is, therefore, the most relevant small mammal species for higher tier risk assessment.
Photoprotective Strategies in Mediterranean High-Mountain Grasslands
Authors: Magaña Ugarte Rosina , Gómez-Serranillos María Pilar , Escudero Adrián , Gavilán Rosario G.
Published: March 2021

Albeit the remarkably high Ultraviolet B loads, high temperatures, and drought stress substantiate the need for efficient photoprotective strategies in Mediterranean high-mountain plants, these remain understudied. Considering the sensitivity of photosystems to extreme conditions, we evaluated an environmental gradient’s weight on the photoprotection of five high-mountain specialists from Central Spain. Diurnal and seasonal variations in chlorophyll, chlorophyll fluorescence, carotenoids, and xanthophylls in consecutive and climatically contrasting years were taken to evaluate the effect of the impending climate coarsening at the photosystem level. Our results revealed significant differences among species in the xanthophyll cycle functioning, acting either as a continuous photoprotective strategy enhancing photochemistry-steadiness; or prompted only to counteract the cumulative effects of atypically adverse conditions. The lutein cycle’s involvement is inferred from the high lutein content found in all species and elevations, acting as a sustained photoprotective strategy. These findings added to high de-epoxidation state (DEPS) and minor seasonal changes in the chlorophyll a/b ratio, infer the xanthophyll and Lutein cycles are crucial for upkeeping the photosystems’ optimal functioning in these plants heightening their photoprotective capacity during periods of more unfavorable conditions. Nevertheless, an atypically dry growing season’s detrimental effect infers the feasible surpassing of stress-thresholds and the precariousness of the communities’ functional diversity under climate change.
Strength and Performance Tests for Screening Reduced Muscle Mass in Elderly Lebanese Males with Obesity in Community Dwellings
Authors: Saadeddine Dana , Itani Leila , Rossi Andrea P. , Pellegrini Massimo , El Ghoch Marwan
Published: March 2021

The reduction in skeletal muscle mass (SMM) is a common phenomenon in older adults. It is associated with several diseases, a reduction in physical fitness, longer periods of hospitalization and high rates of mortality. We aimed to identify the reliability of simple tools for screening for reduced SMM among older adult males in Lebanon. The Tanita MC-780MA bioimpedance analyzer (BIA) was used to assess body composition in a population of 102 community-dwelling elderly males with overweight or obesity, in order to be then categorized as with or without reduced SMM. Participants also performed the handgrip strength test and the 4 m gait speed test. Of the total sample of 102 participants (mean age 67.4 ± 6.96 years; BMI 30.8 6 ± 4.04 kg/m2), 32 (31.4%) met the criteria for reduced SMM. Partial correlation analysis showed that handgrip strength (ρ = 0.308, p = 0.002) and 4 m gait speed (ρ = 0.284, p = 0.004) were both associated with low SMM. Receiver operating characteristic (ROC) curve analysis identified discriminating cut-off points of 1.1 m/s for the 4 m gait speed test and 32.0 kg for the handgrip strength test. Our study showed that participants displayed a substantial prevalence of reduced SMM. Reduced 4 m gait speed and handgrip strength were associated with low SMM. Clear cut-off points for strength and functional tests for screening for this condition in Lebanese older men were identified.
Drought Stress Can Induce the Pathogenicity of Cryptostroma corticale, the Causal Agent of Sooty Bark Disease of Sycamore Maple
Authors: Ogris Nikica , Brglez Ana , Piškur Barbara
Published: March 2021

Reports of sooty bark disease of maples caused by the fungus Cryptostroma corticale have recently been emerging from across Europe. The aims of our study were to describe the first report of sooty bark disease in Slovenia, to determine the pathogenicity of C. corticale, to confirm the optimum temperature for the growth of the fungus, and to determine the mass loss of Acer pseudoplatanus wood inoculated by C. corticale. We confirmed the presence of C. corticale on A. pseudoplatanus via morphological and molecular analysis. The optimal growth of C. corticale was measured in vitro on potato dextrose agar and was determined to occur at 25 °C. Pathogenicity tests were performed on 30 saplings of A. pseudoplatanus under two treatments, humid and drought stress, and the fungus was pathogenic in both treatments. The mean length of bark lesions and wood discoloration of the drought-stressed saplings was significantly greater than that in the humid treatment. Re-isolations of C. corticale were successful from all inoculated saplings, and thus Koch’s postulates were confirmed. The mass loss of A. pseudoplatanus wood was determined by mini-block test in a period of 10 weeks and was observed as minimal. Based on the results, we conclude that C. corticale is a weak and opportunistic pathogen that most likely expresses itself intensively under hot and dry conditions.
RecPOID: POI Recommendation with Friendship Aware and Deep CNN
Authors: Safavi Sadaf , Jalali Mehrdad
Published: March 2021

In location-based social networks (LBSNs), exploit several key features of points-of-interest (POIs) and users on precise POI recommendation be significant. In this work, a novel POI recommendation pipeline based on the convolutional neural network named RecPOID is proposed, which can recommend an accurate sequence of top-k POIs and considers only the effect of the most similar pattern friendship rather than all user’s friendship. We use the fuzzy c-mean clustering method to find the similarity. Temporal and spatial features of similar friends are fed to our Deep CNN model. The 10-layer convolutional neural network can predict longitude and latitude and the Id of the next proper locations; after that, based on the shortest time distance from a similar pattern’s friendship, select the smallest distance locations. The proposed structure uses six features, including user’s ID, month, day, hour, minute, and second of visiting time by each user as inputs. RecPOID based on two accessible LBSNs datasets is evaluated. Experimental outcomes illustrate considering most similar friendship could improve the accuracy of recommendations and the proposed RecPOID for POI recommendation outperforms state-of-the-art approaches.
Multifunctional Tyrosinase Inhibitor Peptides with Copper Chelating, UV-Absorption and Antioxidant Activities: Kinetic and Docking Studies
Authors: Yap Pei-Gee , Gan Chee-Yuen
Published: March 2021

Nature-derived tyrosinase inhibitors are of great industrial interest. Three monophenolase inhibitor peptides (MIPs) and three diphenolase inhibitor peptides (DIPs) from a previous study were investigated for their in vitro tyrosinase inhibitory effects, mode of inhibition, copper-chelating activity, sun protection factor (SPF) and antioxidant activities. DIP1 was found to be the most potent tyrosinase inhibitor (IC50 = 3.04 ± 0.39 mM), which could be due to the binding interactions between its aromatic amino acid residues (Y2 and D7) with tyrosinase hotspots (H85, V248, H258, H263, F264, R268, V283 and E322) and its ability to chelate copper ion within the substrate-binding pocket. The conjugated planar rings of tyrosine and tryptophan may interact with histidine within the active site to provide stability upon enzyme-peptide binding. This postulation was later confirmed as the Lineweaver–Burk analysis had identified DIP1 as a competitive inhibitor and DIP1 also showed 36.27 ± 1.17% of copper chelating activity. In addition, DIP1 provided the highest SPF value (11.9 ± 0.04) as well as ferric reducing antioxidant power (FRAP) (5.09 ± 0.13 mM FeSO4), 2,2′–azinobis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) (11.34 ± 0.90%) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (29.14 ± 1.36%) free radical scavenging activities compared to other peptides. These results demonstrated that DIP1 could be a multifunctional anti-tyrosinase agent with pharmaceutical and cosmeceutical applications.
Optimal Incentives Schemes under Homo Moralis Preferences
Authors: Sarkisian Roberto
Published: March 2021

This study focuses on the optimal incentive schemes in a multi-agent moral hazard model, where each agent has other-regarding preferences and an individual measure of output, with both being observable by the principal. In particular, the two agents display homo moralis preferences. I find that, contrary to the case with purely selfish preferences, tournaments can never be optimal when agents are risk averse, and as the degree of morality increases, positive payments are made in a larger number of output realizations. Furthermore, I extend the analysis to a dynamic setting, in which a contract is initially offered to the agents, who then repeatedly choose which level of effort to provide in each period. I show that the optimal incentive schemes in this case are similar to the ones obtained in the static setting, but for the role of intertemporal discounting.
Biopolymers Hybrid Particles Used in Dentistry
Authors: Chen I-Hao , Lee Tzer-Min , Huang Chih-Ling
Published: March 2021

This literature review provides an overview of the fabrication and application of biopolymer hybrid particles in dentistry. A total of 95 articles have been included in this review. In the review paper, the common inorganic particles and biopolymers used in dentistry are discussed in general, and detailed examples of inorganic particles (i.e., hydroxyapatite, calcium phosphate, and bioactive glass) and biopolymers such as collagen, gelatin, and chitosan have been drawn from the scientific literature and practical work. Among the included studies, calcium phosphate including hydroxyapatite is the most widely applied for inorganic particles used in dentistry, but bioactive glass is more applicable and multifunctional than hydroxyapatite and is currently used in clinical practice. Today, biopolymer hybrid particles are receiving more attention as novel materials for several applications in dentistry, such as drug delivery systems, bone repair, and periodontal regeneration surgery. The literature published on the biopolymer gel-assisted synthesis of inorganic particles for dentistry is somewhat limited, and therefore, this article focuses on reviewing and discussing the biopolymer hybrid particles used in dentistry.
DNA Methylation at ATP11A cg11702988 Is a Biomarker of Lung Disease Severity in Cystic Fibrosis: A Longitudinal Study
Authors: Pineau Fanny , Caimmi Davide , Taviaux Sylvie , Reveil Maurane , Brosseau Laura , Rivals Isabelle , Drevait Margot , Vachier Isabelle , Claustres Mireille , Chiron Raphaël , De Sario Albertina
Published: March 2021

Cystic fibrosis (CF) is a chronic genetic disease that mainly affects the respiratory and gastrointestinal systems. No curative treatments are available, but the follow-up in specialized centers has greatly improved the patient life expectancy. Robust biomarkers are required to monitor the disease, guide treatments, stratify patients, and provide outcome measures in clinical trials. In the present study, we outline a strategy to select putative DNA methylation biomarkers of lung disease severity in cystic fibrosis patients. In the discovery step, we selected seven potential biomarkers using a genome-wide DNA methylation dataset that we generated in nasal epithelial samples from the MethylCF cohort. In the replication step, we assessed the same biomarkers using sputum cell samples from the MethylBiomark cohort. Of interest, DNA methylation at the cg11702988 site (ATP11A gene) positively correlated with lung function and BMI, and negatively correlated with lung disease severity, P. aeruginosa chronic infection, and the number of exacerbations. These results were replicated in prospective sputum samples collected at four time points within an 18-month period and longitudinally. To conclude, (i) we identified a DNA methylation biomarker that correlates with CF severity, (ii) we provided a method to easily assess this biomarker, and (iii) we carried out the first longitudinal analysis of DNA methylation in CF patients. This new epigenetic biomarker could be used to stratify CF patients in clinical trials.
Pilot Study to Detect Genes Involved in DNA Damage and Cancer in Humans: Potential Biomarkers of Exposure to E-Cigarette Aerosols
Authors: Hamad Samera H. , Brinkman Marielle C. , Tsai Yi-Hsuan , Mellouk Namya , Cross Kandice , Jaspers Ilona , Clark Pamela I. , Granville Courtney A.
Published: March 2021

There is a paucity of data on how gene expression enables identification of individuals who are at risk of exposure to carcinogens from e-cigarette (e-cig) vaping; and how human vaping behaviors modify these exposures. This pilot study aimed to identify genes regulated from acute exposure to e-cig using RT-qPCR. Three subjects (2M and 1F) made three visits to the lab (nTOT = 9 visits); buccal and blood samples were collected before and immediately after scripted vaping 20 puffs (nTOT = 18 samples); vaping topography data were collected in each session. Subjects used their own e-cig containing 50:50 propylene glycol (PG):vegetable glycerine (VG) +3–6 mg/mL nicotine. The tumor suppressor TP53 was significantly upregulated in buccal samples. TP53 expression was puff volume and flow rate dependent in both tissues. In blood, the significant downregulation of N-methylpurine DNA glycosylase (MPG), a base excision repair gene, was consistent across all subjects. In addition to DNA repair pathway, cell cycle and cancer pathways were the most enriched pathways in buccal and blood samples, respectively. This pilot study demonstrates that vaping 20 puffs significantly alters expression of TP53 in human tissues; vaping behavior is an important modifier of this response. A larger study is needed to confirm these relationships.
Identification of Chlorophyll Metabolism- and Photosynthesis-Related Genes Regulating Green Flower Color in Chrysanthemum by Integrative Transcriptome and Weighted Correlation Network Analyses
Authors: Fu Hansen , Zeng Tuo , Zhao Yangyang , Luo Tingting , Deng Huijie , Meng Chenwei , Luo Jing , Wang Caiyun
Published: March 2021

Green chrysanthemums are difficult to breed but have high commercial value. The molecular basis for the green petal color in chrysanthemum is not fully understood. This was investigated in the present study by RNA sequencing analysis of white and green ray florets collected at three stages of flower development from the F1 progeny of the cross between Chrysanthemum × morifolium “Lüdingdang” with green-petaled flowers and Chrysanthemum vistitum with white-petaled flowers. The chlorophyll content was higher and chloroplast degradation was slower in green pools than in white pools at each developmental stage. Transcriptome analysis revealed that genes that were differentially expressed between the two pools were enriched in pathways related to chlorophyll metabolism and photosynthesis. We identified the transcription factor genes CmCOLa, CmCOLb, CmERF, and CmbHLH as regulators of the green flower color in chrysanthemum by differential expression analysis and weighted gene co-expression network analysis. These findings can guide future efforts to improve the color palette of chrysanthemum flowers through genetic engineering.
Modulation of Ion Transport to Restore Airway Hydration in Cystic Fibrosis
Authors: Reihill James , Douglas Lisa , Martin S.
Published: March 2021

Cystic fibrosis (CF) is a life-limiting genetic disorder caused by loss-of-function mutations in the gene which codes for the CF transmembrane conductance regulator (CFTR) Cl− channel. Loss of Cl− secretion across the apical membrane of airway lining epithelial cells results in dehydration of the airway surface liquid (ASL) layer which impairs mucociliary clearance (MCC), and as a consequence promotes bacterial infection and inflammation of the airways. Interventions that restore airway hydration are known to improve MCC. Here we review the ion channels present at the luminal surface of airway epithelial cells that may be targeted to improve airway hydration and MCC in CF airways.
Noninvasive Prenatal Paternity Testing with a Combination of Well-Established SNP and STR Markers Using Massively Parallel Sequencing
Authors: Shen Xuefeng , Li Ran , Li Haixia , Gao Yu , Chen Hui , Qu Ning , Peng Dan , Wu Riga , Sun Hongyu
Published: March 2021

Cell-free fetal DNA (cffDNA) from maternal plasma has made it possible to develop noninvasive prenatal paternity testing (NIPPT). However, most studies have focused on customized single nucleotide polymorphism (SNP) typing systems and few have used conventional short tandem repeat (STR) markers. Based on massively parallel sequencing (MPS), this study used a widely-accepted forensic multiplex assay system to evaluate the effect of noninvasive prenatal paternity testing with a combination of well-established SNP and STR markers. Using a ForenSeq DNA Signature Prep Kit, NIPPT was performed in 17 real parentage cases with monovular unborn fetuses at 7 to 24 gestational weeks. Different analytical strategies for the identification of paternally inherited allele (PIA) were developed to deal with SNPs and STRs. Combined paternity index (CPI) for 17 real trios as well as 272 unrelated trios was calculated. With the combination of SNPs and A-STRs, 82.35% (14/17), 88.24% (15/17), 94.12% (16/17), and 94.12% (16/17) of real trios could be accurately determined when the likelihood ratio (LR) threshold for paternity inclusion was set to 10,000, 1000, 100, and 10, respectively. This reveals that simultaneous surveys of SNP and STR markers included in the ForenSeq DNA Signature Prep Kit offer a promising method for NIPPT using MPS technology.
Rapid Support for Older Adults during the Initial Stages of the COVID-19 Pandemic: Results from a Geriatric Psychiatry Helpline
Authors: Wahl Anna-Sophia , Benson Gloria , Hausner Lucrezia , Schmitt Sandra , Knoll Annika , Ferretti-Bondy Adriana , Hefter Dimitri , Froelich Lutz
Published: March 2021

Background. The COVID-19 pandemic and governmental lockdown measures disproportionally impact older adults. This study presents the results from a psychiatric helpline for older adults in Mannheim, Germany, during the lockdown, set up to provide information and psychosocial support. We aim to elucidate the needs of older adults, their reported changes, and the psychological impact during the initial stages of the health crisis. Methods: A total of 55 older adults called the psychiatric helpline between April and June 2020. Information on demographics, medical and psychiatric history. as well as changes in daily life due to the pandemic was collected anonymously. Mental health status was assessed using the 7-Item Hamilton Depression Rating Scale (HAMD-7) and the Hamilton Anxiety Rating Scale (HAM-A). Results: Most callers were women, older adults (M = 74.69 years), single, and retired. In total, 69% of callers reported new or an increase in psychiatric symptoms, with anxiety and depressive symptoms being the most common ones. Age was significantly negatively correlated to higher levels of anxiety and depression symptoms. Individuals with a previous diagnosis of a psychiatric disease reported significantly higher levels of depressive and anxiety symptoms than those without a diagnosis. Conclusion: In older adults, the perceived psychological impact of the COVID-19 crisis appears to ameliorate with age. Individuals with a history of psychiatric disease are most vulnerable to negative mental health outcomes. Rapid response in the form of a geriatric helpline is a useful initiative to support the psychosocial needs of older adults during a health crisis.
Dynamics of Public Spending on Health and Socio-Economic Development in the European Union: An Analysis from the Perspective of the Sustainable Development Goals
Authors: Peña-Sánchez Antonio Rafael , Ruiz-Chico José , Jiménez-García Mercedes
Published: March 2021

In recent years, healthcare has become a fundamental pillar of the level of well-being of any society. With the aim of improving the lives of countries and societies, in 2015 the United Nations (UN) approved the 2030 Agenda for Sustainable Development. Among the Sustainable Development Goals (SDGs) set out in the Agenda are health and well-being (O3) and the reduction of inequalities (O10). The general objective of this paper is to analyse the impact that the level of socioeconomic development, as well as the evolution of inequalities, have had on public spending on health in European Union countries. The research methodology is based on the application of a regression model and statistical techniques such as sigma convergence, beta convergence and the Gini index. We can see that the levels of public spending on health per capita, the level of socio-economic development and the degree of inequality are closely related in these countries. For this reason, we suggest maintaining sustainable economic growth to reduce the economic disparities between EU countries, and also the current differences in public spending on health per capita.
Living Mulch with Selected Herbs for Soil Management in Organic Apple Orchards
Authors: Mia Md , Furmanczyk Ewa , Golian Joanna , Kwiatkowska Joanna , Malusá Eligio , Neri Davide
Published: March 2021

The establishment of living mulches in organic orchards could potentially improve the orchard biodiversity and, when specific plant species are selected, provide additional eco-services and functions, including adequate weed management. This study was conducted in an organically managed apple orchard in Skierniewice (Poland) to assess the effect of two selected living mulching species: Alchemilla vulgaris and Mentha piperita. They were assessed on weed control, weed biodiversity, tree nutritional status, root dry weight density (RDWD), and other root morphological traits compared to a natural soil cover (control). Overall, both living mulches produced 42.5% more dry biomass, increased weed species number (+29%), and increased soil coverage (+33%) compared to control mowed plots. The apple leaf chlorophyll index and nutrient content were higher in the presence of both living mulches than in the control. In addition, apple trees had 30–46% higher root dry weight densities, even though other root morphological traits were not affected by the treatments. The results suggested that the tree row can be managed with living mulches of herbs; these species have the potential to provide an additional income to the farmer, as well as beneficial effects for the orchard biodiversity, without impairing the tree root development and nutrient status.
A Decentralized Semantic Reasoning Approach for the Detection and Representation of Continuous Spatial Dynamic Phenomena in Wireless Sensor Networks
Authors: Ntankouo Njila Roger Cesarié , Mostafavi Mir Abolfazl , Brodeur Jean
Published: March 2021

In this paper, we propose a decentralized semantic reasoning approach for modeling vague spatial objects from sensor network data describing vague shape phenomena, such as forest fire, air pollution, traffic noise, etc. This is a challenging problem as it necessitates appropriate aggregation of sensor data and their update with respect to the evolution of the state of the phenomena to be represented. Sensor data are generally poorly provided in terms of semantic information. Hence, the proposed approach starts with building a knowledge base integrating sensor and domain ontologies and then uses fuzzy rules to extract three-valued spatial qualitative information expressing the relative position of each sensor with respect to the monitored phenomenon’s extent. The observed phenomena are modeled using a fuzzy-crisp type spatial object made of a kernel and a conjecture part, which is a more realistic spatial representation for such vague shape environmental phenomena. The second step of our approach uses decentralized computing techniques to infer boundary detection and vertices for the kernel and conjecture parts of spatial objects using fuzzy IF-THEN rules. Finally, we present a case study for urban noise pollution monitoring by a sensor network, which is implemented in Netlogo to illustrate the validity of the proposed approach.
Aromatic Amino Acid Decarboxylase Deficiency: The Added Value of Biochemistry
Authors: Montioli Riccardo , Borri Voltattorni Carla
Published: March 2021

Aromatic amino acid decarboxylase (AADC) deficiency is a rare, autosomal recessive neurometabolic disorder caused by mutations in the DDC gene, leading to a deficit of AADC, a pyridoxal 5′-phosphate requiring enzyme that catalyzes the decarboxylation of L-Dopa and L-5-hydroxytryptophan in dopamine and serotonin, respectively. Although clinical and genetic studies have given the major contribution to the diagnosis and therapy of AADC deficiency, biochemical investigations have also helped the comprehension of this disorder at a molecular level. Here, we reported the steps leading to the elucidation of the functional and structural features of the enzyme that were useful to identify the different molecular defects caused by the mutations, either in homozygosis or in heterozygosis, associated with AADC deficiency. By revisiting the biochemical data available on the characterization of the pathogenic variants in the purified recombinant form, and interpreting them on the basis of the structure-function relationship of AADC, it was possible: (i) to define the enzymatic phenotype of patients harboring pathogenic mutations and at the same time to propose specific therapeutic managements, and (ii) to identify residues and/or regions of the enzyme relevant for catalysis and/or folding of AADC.
Neonatal Urine Screening Program in the Province of Quebec: Technological Upgrade from Thin Layer Chromatography to Tandem Mass Spectrometry
Authors: Auray-Blais Christiane , Boutin Michel , Lavoie Pamela , Maranda Bruno
Published: March 2021

The Quebec Neonatal Urine Screening Program was initiated in 1971 with overall screening inception of newborns in 1973. Forty-seven years later, over 3.5 million babies have been screened for up to 25 inborn errors of metabolism divided into two groups: (1) urea cycle disorders and organic acidurias; and (2) disorders of amino acid metabolism and transport. The main goal of this preventive genetic medicine program is the detection of treatable diseases before the onset of clinical symptoms. Urine specimens from 21-day-old babies are collected and dried on filter paper by parents at home. The participation is voluntary with a high compliance rate over the years (~90%). Specimens are analyzed by thin layer chromatography (TLC). The main objective of this evaluative research project was to assess the feasibility of a technological upgrade towards mass spectrometry. A 2.85-min flow injection method was devised, normal values established, and abnormal profiles confirmed using second-tier tests. The validated assays are sensitive, specific, and suitable for populational screening, as well as for high-risk screening laboratories. Triple H syndrome, which would not be detected in newborns by blood screening at two days of age was found to be positive in the urine of an affected patient.
Representation of Slovak Research Information (A Case Study)
Authors: Zendulková Danica , Rysuľa Boris , Putalová Andrea
Published: March 2021

In the light of the increasing importance of the societal impact of research, this article attempts to address the question as to how social sciences and humanities (SSH) research outputs from 2019 are represented in Slovak research portfolios in comparison with those of the EU-28 and the world. The data used for the analysis originate from the R&D SK CRIS and bibliographic Central Register of Publication Activities (CREPČ) national databases, and WoS Core Collection/InCites. The research data were appropriate for the analysis at the time they were structured, on the national level; of high quality and consistency; and covering as many components as possible and in mutual relations. The data resources should enable the research outputs to be assigned to research categories. The analysis prompts the conclusion that social sciences and humanities research outputs in Slovakia in 2019 are appropriately represented and in general show an increasing trend. This can be documented by the proportion represented by the SSH research projects and other entities involved in the overall Slovak research outputs, and even the higher ratio of SSH research publications in comparison with the EU-28 and the world. Recommendations of a technical character include research data management, data quality, and the integration of individual systems and available analytical tools.
Small Bait Traps May Not Accurately Reflect the Composition of Necrophagous Diptera Associated to Remains
Authors: LeBlanc Kathleen , Boudreau Denis R. , Moreau Gaétan
Published: March 2021

Small bait traps are beginning to emerge in forensic entomology as a new approach to sample early-colonizing necrophagous Diptera species while reducing the investment in time and energy in obtaining information. To test the hypothesis conveyed by the literature that these traps can be a substitute for whole carcasses, we simultaneously documented the Diptera assemblages visiting and colonizing domestic pig carcasses and small traps baited with pork liver. Results indicated that Diptera species occurrence and assemblage composition in the small bait traps and on the carcasses differed, while they were similar when comparing only the pig carcasses. These results are in agreement with the literature that examined insect colonization of other decaying substrates. Although small bait traps can be useful tools to document the communities of necrophagous Diptera in a given area, we stress that caution must be exercised when extending the data obtained by these traps to courtroom proceedings.
Salivary Digestion Extends the Range of Sugar-Aversions in the German Cockroach
Authors: Wada-Katsumata Ayako , Schal Coby
Published: March 2021

Saliva has diverse functions in feeding behavior of animals. However, the impact of salivary digestion of food on insect gustatory information processing is poorly documented. Glucose-aversion (GA) in the German cockroach, Blattella germanica, is a highly adaptive heritable behavioral resistance trait that protects the cockroach from ingesting glucose-containing-insecticide-baits. In this study, we confirmed that GA cockroaches rejected glucose, but they accepted oligosaccharides. However, whereas wild-type cockroaches that accepted glucose also satiated on oligosaccharides, GA cockroaches ceased ingesting the oligosaccharides within seconds, resulting in significantly lower consumption. We hypothesized that saliva might hydrolyze oligosaccharides, releasing glucose and terminating feeding. By mixing artificially collected cockroach saliva with various oligosaccharides, we demonstrated oligosaccharide-aversion in GA cockroaches. Acarbose, an alpha-glucosidase inhibitor, prevented the accumulation of glucose and rescued the phagostimulatory response and ingestion of oligosaccharides. Our results indicate that pre-oral and oral hydrolysis of oligosaccharides by salivary alpha-glucosidases released glucose, which was then processed by the gustatory system of GA cockroaches as a deterrent and caused the rejection of food. We suggest that the genetic mechanism of glucose-aversion support an extended aversion phenotype that includes glucose-containing oligosaccharides. Salivary digestion protects the cockroach from ingesting toxic chemicals and thus could support the rapid evolution of behavioral and physiological resistance in cockroach populations.
Reducing Pesticides and Increasing Crop Diversification Offer Ecological and Economic Benefits for Farmers—A Case Study in Cambodian Rice Fields
Authors: Sattler Cornelia , Schrader Julian , Flor Rica Joy , Keo Makarakpakphea , Chhun Sokunroth , Choun Saban , Hadi Buyung Asmara Ratna , Settele Josef
Published: March 2021

Rice production is often associated with high pesticide input. To improve farmers’ practice, sustainable management approaches are urgently needed, such as ecological engineering (EE), which aims at enhancing beneficial arthropods while reducing pesticides. Here, we implemented and tested EE in Cambodian rice fields by comparing: (i) fields not treated with pesticides (control); (ii) fields not treated with pesticides but with non-rice crops planted in the surrounding (EE); and (iii) conventionally farmed fields using pesticides (CR). Using benefit-cost analysis, we compared the economic value of each treatment. The non-rice crops preferred by men and women farmers as well as farmers’ willingness to implement EE were assessed using surveys. We sampled arthropod abundance and richness in rice fields and bunds during two seasons. During the dry season, we compared EE and CR among three Cambodian provinces. During the wet season, we specifically assessed the differences in EE, control and CR in arthropod abundance and rice yield in one province. While withholding from using pesticides did not result in a decrease in yield in EE and control treatments, parasitoid abundance was higher in both treatments during the wet season. The benefit–cost ratio was highest for EE and control treatments. Pesticides were likely the main driver causing low arthropod abundance, without any benefit towards increased rice yield. The proper implementation of EE coupled with farmers’ knowledge of ecologically based pest management is a promising solution towards sustainable rice production.
Synthesis of the Brazilian Poduromorpha (Collembola: Hexapoda) with Special Emphasis on the Equatorial Oceanic Islands
Authors: de Lima Estevam C. A. , de Mendonça Maria Cleide , Queiroz Gabriel Costa , da Silveira Tatiana Cristina , Zeppelini Douglas
Published: March 2021

We present new species and records of Poduromorpha for the Brazilian oceanic islands and synthesis of this order in Brazil. Friesea noronhaensis sp. nov., Friesea rochedoensis sp. nov., Willemia insularum sp. nov. and Paraxenylla zeliae sp. nov. are described and a diagnosis of the morphospecies Acherontiella sp. Lima and Zeppelini 2015 is provided. We present comparative tables, distribution and taxonomic keys of the Friesea, Arlesia, Brachystomella, Acherontiella, Paraxenylla, Xenylla, and Willemia found in the Brazilian oceanic islands and their respective congeners recorded in Brazil.
A Review of Basic Energy Reconstruction Techniques in Liquid Xenon and Argon Detectors for Dark Matter and Neutrino Physics Using NEST
Authors: Szydagis Matthew , Block Grant A. , Farquhar Collin , Flesher Alexander J. , Kozlova Ekaterina S. , Levy Cecilia , Mangus Emily A. , Mooney Michael , Mueller Justin , Rischbieter Gregory R. C. , Schwartz Andrew K.
Published: March 2021

Detectors based upon the noble elements, especially liquid xenon as well as liquid argon, as both single- and dual-phase types, require reconstruction of the energies of interacting particles, both in the field of direct detection of dark matter (weakly interacting massive particles WIMPs, axions, etc.) and in neutrino physics. Experimentalists, as well as theorists who reanalyze/reinterpret experimental data, have used a few different techniques over the past few decades. In this paper, we review techniques based on solely the primary scintillation channel, the ionization or secondary channel available at non-zero drift electric fields, and combined techniques that include a simple linear combination and weighted averages, with a brief discussion of the application of profile likelihood, maximum likelihood, and machine learning. Comparing results for electron recoils (beta and gamma interactions) and nuclear recoils (primarily from neutrons) from the Noble Element Simulation Technique (NEST) simulation to available data, we confirm that combining all available information generates higher-precision means, lower widths (energy resolution), and more symmetric shapes (approximately Gaussian) especially at keV-scale energies, with the symmetry even greater when thresholding is addressed. Near thresholds, bias from upward fluctuations matters. For MeV-GeV scales, if only one channel is utilized, an ionization-only-based energy scale outperforms scintillation; channel combination remains beneficial. We discuss here what major collaborations use.
Development of Carbon Fiber-Reinforced Thermoplastics for Mass-Produced Automotive Applications in Japan
Authors: Wan Yi , Takahashi Jun
Published: March 2021

The application of carbon fiber-reinforced thermoplastics (CFRTPs) for automotive mass production is attracting increasing attention from researchers and engineers in related fields. This article presents recent developments in CFRTPs focusing on the systematic development of lightweight CFRTP applications for automotive mass production. Additionally, a related national project of Japan conducted at the University of Tokyo is also introduced. The basic development demands, the specific requirements of CFRTPs for lightweight applications in automotive mass production, and the current development status and basic scientific outputs are discussed. The development of high-performance CFRTPs (chopped carbon fiber tape-reinforced thermoplastics (CTTs)) and functional CFRTPs (carbon fiber mat-reinforced thermoplastics (CMTs)) is also introduced. The fabrication process control of CTTs is evaluated, which demonstrates the extreme importance of the mechanical performance. The ultralight lattice, toughened structures, and orientation designable components of CMTs provide a flexible multi-material solution for the proposed applications. Moreover, highly efficient carbon fiber recycling technology is discussed, with recycled carbon fibers exhibiting outstanding compatibility with CFRTPs. A cost sensitivity analysis of carbon fiber and CFRTPs is conducted to guarantee the feasibility and affordability of their application. This article also discusses the trends and sustainability of carbon fiber and CFRTPs usage. The importance of the object-oriented optimal development of CFRTPs is emphasized to efficiently exploit their advantages.
Validation of the SavvyCheck™ Vaginal Yeast Test for Screening Pregnant Women for Vulvovaginal Candidosis: A Prospective, Cross-Sectional Study
Authors: Foessleitner Philipp , Kiss Herbert , Deinsberger Julia , Ott Julia , Zierhut Lorenz , Farr Alex
Published: March 2021

Pregnant women have an increased risk of vulvovaginal candidosis. Recurrent candidosis is under debate as a contributor to preterm birth, and vertical transmission may cause diaper dermatitis and oral thrush in the newborn. Apart from cultural methods, the gold standard for diagnosing candidosis is Gram staining, which is time-consuming and requires laboratory facilities. The objective of this prospective study was to validate a point-of-care vaginal yeast detection assay (SavvyCheck™ Vaginal Yeast Test) and to evaluate it in asymptomatic pregnant women. We enrolled 200 participants, 100 of whom had vulvovaginal candidosis according to Gram stain (study group) and 100 were healthy pregnant controls (control group). Of these, 22 participants (11%) had invalid test results. The point-of-care test of the remaining 85 and 93 study participants in the study and control groups, respectively, showed a sensitivity of 94.1%, specificity of 98.9%, positive predictive value of 90.3%, and negative predictive value of 99.4% when compared with Gram stain. In conclusion, we found a high correlation between the SavvyCheck™ Vaginal Yeast Test and Gram-stained smears during pregnancy. This suggests a potential role of this point-of-care test as a screening tool for asymptomatic pregnant women in early gestation.
Assessment of Commercial Fungicides against Onion (Allium cepa) Basal Rot Disease Caused by Fusarium oxysporum f. sp. cepae and Fusarium acutatum
Authors: Degani Ofir , Kalman Ben
Published: March 2021

The onion basal rot disease is a worldwide threat caused by species of the genus Fusarium. Today, Israel’s control of this disease is limited to a four-year growth cycle and Metam sodium soil disinfection. Here, commercial chemical fungicides were evaluated as control treatments against two of the primary pathogens involved, F. oxysporum f. sp. cepae and F. Acutatum. Out of 10 fungicides tested on culture plates, 3, Prochloraz, Azoxystrobin + Tebuconazole, and Fludioxonil + Sedaxen, had strong inhibitory effects on mycelial growth and were selected and tested in seeds in vitro. The preparations were applied as a seed coating and tested in two commercial cultivars, Riverside (Orlando, white cv.) and Noam (red cv.). Prochloraz (0.3% w/w concentration), the most promising compound, was efficient in reducing the Noam cv. sprouts’ disease symptoms. This preparation had no harmful in situ-toxicity effect and did not influence the plants’ seed germination and early development. In Noam cv. potted 30-day-old sprouts, the Prochloraz treatment was able to reduce the harmful impact of F. oxysporum f. sp. cepae. on the seedlings’ wet biomass, but was not effective in the Riverside cv. or against the F. acutatum pathogen. This suggests that future protective strategies must include an effective protective suit tailored to each of the pathogen species involved and the onion cultivar. The methods presented in this work can be applied for rapidly scanning multiple compounds while gradually ruling out ineffective ones. Eventually, this screening will enable field testing of the highest potential fungicides that successfully pass the pot experiments.
Estimating the Growth Effects of 2004 Eastern Enlargement of the European Union
Authors: Cieślik Andrzej , Turgut Mehmet Burak
Published: March 2021

In this paper, we study the growth effects of the 2004 Eastern enlargement of the European Union (EU) using the synthetic control method. We estimate that this EU enlargement had an immediate but modest positive impact on the economic growth of the EU-8 countries in the first few years following their EU accession. The positive impact of the EU enlargement became more apparent from 2007 when the new EU member states were admitted into the Schengen zone. As a result, the gross domestic product (GDP) per capita difference between the actual and synthetic EU-8 continued to grow towards the end of the sample period. We found that over the entire 2004–2012 period, GDP per capita of the EU-8 was increased by about 2313 USD per year on average relative to the synthetic EU-8. The growth rate of the GDP per capita in the actual EU-8 for the same period was 2.7% larger than the synthetic EU-8.
Pricing Perpetual American Put Options with Asset-Dependent Discounting
Authors: Al-Hadad Jonas , Palmowski Zbigniew
Published: March 2021

The main objective of this paper is to present an algorithm of pricing perpetual American put options with asset-dependent discounting. The value function of such an instrument can be described as VAPutω(s)=supτ∈TEs[e−∫0τω(Sw)dw(K−Sτ)+], where T is a family of stopping times, ω is a discount function and E is an expectation taken with respect to a martingale measure. Moreover, we assume that the asset price process St is a geometric Lévy process with negative exponential jumps, i.e., St=seζt+σBt−∑i=1NtYi. The asset-dependent discounting is reflected in the ω function, so this approach is a generalisation of the classic case when ω is constant. It turns out that under certain conditions on the ω function, the value function VAPutω(s) is convex and can be represented in a closed form. We provide an option pricing algorithm in this scenario and we present exact calculations for the particular choices of ω such that VAPutω(s) takes a simplified form.
Well-Being Impact on Banking Systems
Authors: Iuga Iulia , Dragolea Larisa-Loredana
Published: March 2021

The present research focuses on the influence of the well-being indicators, more specifically, the indicators reflecting the life quality on the banking systems evolution from the EU member states. The study offers a unique approach to comparing the two country groups: the eurozone countries and the EU noneuro countries during the 2008–2019 period. The model is estimated with the help of the OLS method by using panel data. The study aims to identify which life quality indicators significantly influence the EU member states’ banking systems evolution and develop models dividing the countries into two groups. Our conclusions show that, among all the determinant factors analysed in this study, household consumption and internet users strongly influence all EU countries’ banking systems.
Exploring Cross-Linguistic Effects and Phonetic Interactions in the Context of Bilingualism: Introducing the Special Issue
Authors: Amengual Mark
Published: March 2021

Bilinguals who have acquired both of their languages simultaneously since birth or have learned their first language (L1) and their second language (L2) sequentially, as children or as adults, are able to produce and perceive two different sound systems [...]