bluuebunny/crossref_metadata_2025 huggingface dataset:
Resting State Hypoconnectivity of Reward Networks in Binge Eating Disorder
Authors: Haynos Ann F , Camchong Jazmin , Pearson Carolyn M , Lavender Jason M , Mueller Bryon A , Peterson Carol B , Specker Sheila , Raymond Nancy , Lim Kelvin O
Published: November 2020

The clinical presentation of binge eating disorder (BED) and data emerging from task-based functional neuroimaging research suggests that this disorder may be associated with alterations in reward processing. However, there is a dearth of research investigating the functional organization of brain networks that mediate reward in BED. To address this gap, 27 adults with BED and 21 weight-matched healthy controls (WMC) completed a multimodel assessment consisting of a resting functional magnetic resonance imaging scan, behavioral tasks measuring reward-based decision-making (i.e., delay discounting and reversal learning), and self-report assessing clinical symptoms. A seed-based approach was employed to examine the resting state functional connectivity (rsFC) of the striatum (nucleus accumbens [NAcc] and ventral and dorsal caudate), a collection of regions implicated in reward processing. Compared with WMC, the BED group exhibited lower rsFC of striatal seeds, with frontal regions mediating executive functioning (e.g., superior frontal gyrus [SFG]) and posterior, parietal, and temporal regions implicated in emotional processing. Lower NAcc–SFG rsFC was associated with more difficulties with reversal learning and binge eating frequency in the BED group. Results suggest that hypoconnectivity of striatal networks that integrate self-regulation and reward processing may promote the clinical phenomenology of BED. Interventions for BED may benefit from targeting these circuit-based disturbances.
Coinfection by Severe Acute Respiratory Syndrome Coronavirus 2 and Influenza A(H1N1)pdm09 Virus Enhances the Severity of Pneumonia in Golden Syrian Hamsters
Authors: Zhang Anna Jinxia , Lee Andrew Chak-Yiu , Chan Jasper Fuk-Woo , Liu Feifei , Li Can , Chen Yanxia , Chu Hin , Lau Siu-Ying , Wang Pui , Chan Chris Chung-Sing , Poon Vincent Kwok-Man , Yuan Shuofeng , To Kelvin Kai-Wang , Chen Honglin , Yuen Kwok-Yung
Published: November 2020

Background

Clinical outcomes of the interaction between the co-circulating pandemic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and seasonal influenza viruses are unknown.

Methods

We established a golden Syrian hamster model coinfected by SARS-CoV-2 and mouse-adapted A(H1N1)pdm09 simultaneously or sequentially. The weight loss, clinical scores, histopathological changes, viral load and titer, and serum neutralizing antibody titer were compared with hamsters challenged by either virus.

Results

Coinfected hamsters had more weight loss, more severe lung inflammatory damage, and tissue cytokine/chemokine expression. Lung viral load, infectious virus titers, and virus antigen expression suggested that hamsters were generally more susceptible to SARS-CoV-2 than to A(H1N1)pdm09. Sequential coinfection with A(H1N1)pdm09 one day prior to SARS-CoV-2 exposure resulted in a lower lung SARS-CoV-2 titer and viral load than with SARS-CoV-2 monoinfection, but a higher lung A(H1N1)pdm09 viral load. Coinfection also increased intestinal inflammation with more SARS-CoV-2 nucleoprotein expression in enterocytes. Simultaneous coinfection was associated with delay in resolution of lung damage, lower serum SARS-CoV-2 neutralizing antibody, and longer SARS-CoV-2 shedding in oral swabs compared to that of SARS-CoV-2 monoinfection.

Conclusions

Simultaneous or sequential coinfection by SARS-CoV-2 and A(H1N1)pdm09 caused more severe disease than monoinfection by either virus in hamsters. Prior A(H1N1)pdm09 infection lowered SARS-CoV-2 pulmonary viral loads but enhanced lung damage. Whole-population influenza vaccination for prevention of coinfection, and multiplex molecular diagnostics for both viruses to achieve early initiation of antiviral treatment for improvement of clinical outcome should be considered.
The Future of Wednesbury Unreasonableness in the Substantive Review of Administrative Discretion: A Hong Kong Perspective
Authors: Ramsden Michael
Published: November 2020

The continued use of Wednesbury unreasonableness in the substantive review of administrative discretion has received considerable scholarly attention throughout the common law world. Recent local developments in proportionality review bring this debate to the fore in Hong Kong. It has been argued that the Court of Final Appeal’s articulation of a sliding scale of proportionality review has strengthened the case for the formal abolition of Wednesbury unreasonableness, on the basis that proportionality has now embraced an identical unreasonableness standard. This article challenges the claim that Wednesbury is now a redundant concept in Hong Kong public law. Descriptively, there remain material differences between Wednesbury and proportionality, even under its modified deferential form. Normatively, too, Wednesbury remains justified as a means to recognize the limited general basis in which common law substantive review can occur. A conflation of Wednesbury unreasonableness and proportionality can have the unintended consequence of diluting constitutional protections. Furthermore, the emergence of a sliding scale of Wednesbury review in Hong Kong reduces, rather than increases, pressure for its abolition in favour of proportionality.
Creating a Metabolic Syndrome Research Resource using the National Health and Nutrition Examination Survey
Authors: Jenkins Willysha S , Richardson Christian , Williams Ariel , Williams-DeVane Clarlynda R
Published: November 2020

Metabolic syndrome (MetS) is multifaceted. Risk factors include visceral adiposity, dyslipidemia, hyperglycemia, hypertension and environmental stimuli. MetS leads to an increased risk of cardiovascular disease, type 2 diabetes and stroke. Comparative studies, however, have identified heterogeneity in the pathology of MetS across groups though the etiology of these differences has yet to be elucidated. The Metabolic Syndrome Research Resource (MetSRR) described in this report is a curated database that provides access to MetS-associated biological and ancillary data and pools current and potential biomarkers of MetS extracted from relevant National Health and Nutrition Examination Survey (NHANES) data from 1999–2016. Each potential biomarker was selected following the review of over 100 peer-reviewed articles. MetSRR includes 28 demographics, survey and known MetS-related variables, including 9 curated categorical variables and 42 potentially novel biomarkers. All measures are captured from over 90 000 individuals. This biocuration effort provides increased access to curated MetS-related data and will serve as a hypothesis-generating tool to aid in novel biomarker discovery. In addition, MetSRR provides the ability to generate and export ethnic group-/race-, sex- and age-specific curated datasets, thus broadening participation in research efforts to identify clinically evaluative MetS biomarkers for disparate populations. Although there are other databases, such as BioM2MetDisease, designed to explore metabolic diseases through analysis of miRNAs and disease phenotypes, MetSRR is the only MetS-specific database designed to explore etiology of MetS across groups, through the biocuration of demographic, biological samples and biometric data.

Database URL:  http://www.healthdisparityinformatics.com/MetSRR
A case report of a rare cardiac complication in novel coronavirus disease
Authors: Malekrah Alireza , Fatahian Alireza
Published: November 2020

Background

Since late 2019, the outbreak of COVID-19 has rapidly spread worldwide. As it is a newly emerged disease, many of its manifestations and complications are unknown to us. Cardiac involvement and arrhythmias are another aspect of the disease about which very little is known.

Case summary

A 71-year-old male patient presented at the Emergency Department complaining of fever, a dry cough, and dyspneoa. He was admitted due to these symptoms suggestive of COVID-19, and a chest CT and PCR test confirmed the diagnosis. During admission, cardiac involvement was detected, i.e. second-degree atrioventricular block with intermittent left bundle branch block (LBBB) which progressed to fixed LBBB and eventually developed into atrial fibrillation/flutter with bradycardia. Both cardiac troponin and echocardiographic findings for detecting myocarditis were negative. We waited 14 days for resolution of atrioventricular block before permanent pacemaker implantation, but the condition still did not improve after the waiting period.

Discussion

COVID-19 is mainly a respiratory infection but cardiac involvement is not uncommon in the course of the disease. Arrhythmia, during this infection, seems to be caused by an inflammatory response in the myocardium, electrolyte disturbances, and hypoxia; the course of the disease in our case study shows that the virus can preferentially and irreversibly involve the cardiac conduction system.
One-shot transcatheter double valve replacement: six-month follow-up—a case report
Authors: Lutter Georg , Salem Mohamed , Frank Derk , Puehler Thomas
Published: November 2020

Background

Transcatheter aortic valve replacement (TAVR) in combination with a valve-in-valve (V-i-V) transcatheter mitral valve replacement (TMVR) is a rare procedure in comparison to surgical therapy especially in young patients. We report on a young patient at high surgical risk, receiving a double valve implantation with two S3 transcatheter heart valves.

Case summary

A 59-year-old female patient with two previous mitral valve replacements due to endocarditis and re-endocarditis experienced a new onset of severe mitral valve stenosis in combination with progredient aortic stenosis. She was admitted to the hospital with severe dyspnoea and intermittent non-invasive ventilation [New York Heart Association (NYHA) III–IV]. An interventional transapical transcatheter double valve implantation was planned and carried out due to cardiac decompensation and high comorbidity preoperatively (STS score of 6.92). At 6-month follow-up, the patient presented herself in an improved condition with reduced symptoms (NYHA I–II), a good functional status of both valves and an advanced right and left ventricular function in the echocardiogram.

Discussion

Even in younger patients at high risk, a combined native TAVR and V-i-V TMVR procedure can be performed. In this case, a transcatheter SAPIEN 3 valve was transapically implanted with good clinical mid-term outcome at 6 months.
Impact of electrophysiological features acquired after anatomical repair of congenital corrected transposition of the great arteries on late mortality and ventricular dysfunction
Authors: Ma Kai , Qi Lei , Ren Lan , Zhang Benqing , Liu Rui , Yang Yang , Wang Guanxi , Zhang Sen , Li Shoujun
Published: November 2020

OBJECTIVES

In patients with anatomically repaired congenitally corrected transposition of the great arteries, the impact of electrophysiological features on postoperative ventricular dysfunction remains less well known. Our goal was to investigate the role of fragmented QRS and QRS duration in mortality and systemic ventricular dysfunction after anatomical repair of corrected transposed great arteries.

METHODS

Consecutive patients who underwent anatomical repair in our institution from January 2005 to December 2017 were enrolled in this retrospective analysis. Fragmented QRS was defined as ≥1 discontinuous deflections in narrow QRS complexes, and ≥2 in wide QRS complexes, in 2 contiguous electrocardiogram leads. The primary end point was a composite of all-cause mortality and systemic ventricular dysfunction.

RESULTS

A total of 74 patients were included. Among them, 30, 15 and 29 underwent the Senning arterial switch, the Senning Rastelli and the hemi-Mustard/bidirectional Glenn/Rastelli procedures, respectively. The primary end point occurred in 9 (12.2%) patients and included 7 late deaths and 2 cases of late-onset systemic ventricular dysfunction. Fragmented QRS and QRS prolongation were noted in 19 (25.7%) and 21 (28.4%) patients, respectively. In patients with the primary end point, QRS fragmentation (6/9 vs 10/65; P < 0.001) and QRS prolongation (6/9 vs 15/65; P = 0.013) were noted more frequently than in patients without the primary end point. No statistical differences in these electrocardiogram findings were found among patients treated with 3 surgical strategies.

CONCLUSIONS

Appearance of QRS fragmentation or QRS prolongation is associated with death or ventricular dysfunction in anatomically repaired corrected transposition of the great arteries. Although there is a trend that QRS fragmentation and QRS prolongation appear more frequently in patients who had the Senning-arterial switch operation, there is no statistically significant difference associated with these electrocardiogram features among varied procedures.
Deciphering operation of tryptophan-independent pathway in high indole-3-acetic acid (IAA) producing Micrococcus aloeverae DCB-20
Authors: Ahmad Ees , Sharma Sushil K , Sharma Pawan K
Published: November 2020

Genus Micrococcus is considered a high IAA producer. However, interestingly, there is no report on the tryptophan- independent pathway operation in this genus. Consequently, the present study was undertaken to evaluate high IAA production by Micrococcus aloeverae DCB-20 and generate reasonable evidence for the occurrence of the tryptophan-independent pathway. Strain DCB-20 produced a high quantity of 880.51 µM or 154.3 µg/mL IAA in LB broth supplemented with L-tryptophan. The tryptophan-independent pathway operation was supported by IAA production in Tris-minimal broth (TM broth) medium supplemented with acid hydrolyzed casein hydrolysate (casein acid hydolysate), which lacks tryptophan. The HPLC analysis showed the absence of tryptophan either from exogenous or endogenous sources in TM broth in the presence of casein acid hydrolysate inoculated with M. aloeverae DCB-20. The absence of tryptophan was further confirmed by the appearance of non-pigmented colonies of Chromobacterium violaceum strain TRFM-24 on Tris-minimal agar (TM agar) containing acid-hydrolyzed casein. This is probably the first report on IAA biosynthesis by M. aloeverae DCB-20 employing tryptophan-independent pathway. This simple technique can also be adapted to detect operation of the tryptophan-independent pathway in other bacteria.
Improving xylitol yield by deletion of endogenous xylitol-assimilating genes: a study of industrialSaccharomyces cerevisiaein fermentation of glucose and xylose
Authors: Yang Bai-Xue , Xie Cai-Yun , Xia Zi-Yuan , Wu Ya-Jing , Gou Min , Tang Yue-Qin
Published: November 2020

Engineered Saccharomyces cerevisiae can reduce xylose to xylitol. However, in S.cerevisiae, there are several endogenous enzymes including xylitol dehydrogenase encoded by XYL2, sorbitol dehydrogenases encoded by SOR1/SOR2 and xylulokinase encoded by XKS1 may lead to the assimilation of xylitol. In this study, to increase xylitol accumulation, these genes were separately deleted through CRISPR/Cas9 system. Their effects on xylitol yield of an industrial S. cerevisiae CK17 overexpressing Candida tropicalis XYL1 (encoding xylose reductase) were investigated. Deletion of SOR1/SOR2 or XKS1 increased the xylitol yield in both batch and fed-batch fermentation with different concentrations of glucose and xylose. The analysis of the transcription level of key genes in the mutants during fed-batch fermentation suggests that SOR1/SOR2 are more crucially responsible for xylitol oxidation than XYL2 under the genetic background of S.cerevisiae CK17. The deletion of XKS1 gene could also weaken SOR1/SOR2 expression, thereby increasing the xylitol accumulation. The XKS1-deleted strain CK17ΔXKS1 produced 46.17 g/L of xylitol and reached a xylitol yield of 0.92 g/g during simultaneous saccharification and fermentation (SSF) of pretreated corn stover slurry. Therefore, the deletion of XKS1 gene provides a promising strategy to meet the industrial demands for xylitol production from lignocellulosic biomass.
Racial Disparities in Financial Security, Work and Leisure Activities, and Quality of Life Among the Direct Care Workforce
Authors: Muench Ulrike , Spetz Joanne , Jura Matthew , Harrington Charlene
Published: November 2020

Background and Objectives

Personal care, home health, and nursing aides provide the majority of care to chronically ill and disabled older adults. This workforce faces challenging working conditions, resulting in high turnover and workforce instability that affect the quality of care for older adults. We examine financial security, work–life balance, and quality of life of Black, Hispanic, and workers of other race/ethnicity compared to White workers.

Research Design and Methods

We hypothesize that Black and Hispanic workers experience greater financial insecurity, spend more time on work-related activities and have less time available for leisure activities, and have a lower quality of life compared to White workers. To test these hypotheses, we analyze the American Time Use Survey using descriptive analyses and multivariable and compositional regression.

Results

Black and Hispanic individuals were 2–3 times more likely to live in poverty than White individuals. The time use analysis indicated that Black and Hispanic workers spent more time on work-related activities and less time on nonwork-related activities, including longer work commutes and less time exercising. In analyses of aggregated paid/unpaid work and leisure, Black workers were the only group that spent significantly more time working and less time on leisure activities compared to White workers. This may explain the lower quality of life that we only observed in Black workers.

Discussion and Implications

Racial/ethnic disparities in well-being among direct care workers may affect the care older adults receive and contribute to widening inequities in this workforce and society. Policymakers should direct efforts toward securing funding for workers, incentivizing employer provisions, and implementing racial equity approaches.
O-GlcNAcylation of MEK2 promotes the proliferation and migration of breast cancer cells
Authors: Xu Yaoyao , Sheng Xiangying , Zhao Ting , Zhang Lei , Ruan Yuanyuan , Lu Haojie
Published: November 2020

Mitogen-activated protein kinase kinases are an important part of evolutionary conserved signaling modules that are involved in a variety of cellular processes in response to environmental stimuli. Among them, mitogen-activated protein kinase kinase 2 (MEK2) is the most crucial upstream signaling pathway of ERK1/2 cascade as a therapeutic target for overcoming Ras-driven cancers. However, the mechanisms of MEK2 regulation during tumor progression remain not fully elucidated. Herein, we identified that MEK2 was post-translationally regulated by O-GlcNAcylation. We found that MEK2 associated with OGT and was modified by O-GlcNAc. Mass spectrometry analysis further verified that O-GlcNAcylation of MEK2 occurred at Thr13, which was in the docking domain for specifically identifying its target proteins. While total O-GlcNAcylation stimulated the protein stability and phosphorylation of MEK2, Thr13 O-GlcNAcylation of MEK2 specifically enhanced its Thr394 phosphorylation as well as downstream ERK1/2 activation. Genetic ablation of MEK2 O-GlcNAcylation at Thr13 abrogated its ability to promote the proliferation and migration of breast cancer cells. Together, our data demonstrate that O-GlcNAcylation of MEK2 might be a key regulatory mechanism during tumorigenesis and is a potential therapeutic target for tumor treatment.
Bilateral internal thoracic artery grafting in haemodialysis patients with diabetic nephropathy
Authors: Hachiro Kohei , Kinoshita Takeshi , Suzuki Tomoaki , Asai Tohru
Published: November 2020

OBJECTIVES

To compare postoperative outcomes in patients with diabetic nephropathy receiving haemodialysis and undergoing isolated coronary artery bypass grafting (CABG) using bilateral or single skeletonized internal thoracic artery (ITA).

METHODS

Among 1441 consecutive patients undergoing isolated CABG between 2002 and 2019 at our university hospital, we retrospectively analysed data for 107 patients with diabetic nephropathy receiving haemodialysis. After inverse probability of treatment weighting, we found no statistically significant differences regarding patients’ preoperative characteristics.

RESULTS

All patients underwent myocardial revascularization using the off-pump technique. There was no statistical significance in postoperative deep sternal wound infection (P = 0.902) and 30-day mortality (P = 0.755). However, the bilateral ITA group had a lower rate of postoperative stroke versus the single group (0% vs 5.5%, respectively; P = 0.021). Follow-up was completed in 95.3% (102/107) of the patients, and the mean follow-up duration was 3.3 years. Thirty-eight deaths occurred in the bilateral ITA group and 18 in the single ITA group. There was no significant difference in all-cause death (P = 0.558) and cardiac death rates (P = 0.727). Multivariable Cox regression models showed that the independent predictors of all-cause death were age [hazard ratio (HR) 1.031; P = 0.010], previous percutaneous intervention (HR 1.757; P = 0.009) and gastroepiploic artery grafting (HR 0.582; P = 0.026).

CONCLUSIONS

Bilateral ITA grafting in patients with diabetic nephropathy receiving haemodialysis did not improve mid-term outcomes.
Cardiac anatomy in the ‘Dreyfus Madonna’ by Leonardo da Vinci
Authors: Keshelava Grigol
Published: November 2020

Leonardo’s findings on the anatomy and physiology of the heart, preserved in his private sketches and notes, reveal a remarkable period of transition in the early history of cardiology. The object of this research is a ‘Dreyfus Madonna’. Through the programme Paint X, we moved 1 detail (blue marked) in the direction of the green arrow and we get an image of the heart with branches of the aortic arch. If we compare the image of the heart obtained by us with the anatomical sketch of Leonardo (RL 19073-74v; K/P 1 66v), we will see a sharp resemblance to the branches of the aortic arch.
Administration of Exogenous Progesterone Protects Against Brucella abortus Infection–Induced Inflammation in Pregnant Mice
Authors: Ren Jingjing , Hou Huanhuan , Zhao Weizheng , Wang Jun , Peng Qisheng
Published: November 2020

Progesterone has been recognized as essential for the establishment and maintenance of pregnancy, and is typically known as an immunosuppressive agent. However, its effects on mediating Brucella infection–induced inflammation have not been evaluated. Here we demonstrated that Brucella abortus infection inhibits progesterone levels in the pregnant mouse by suppressing the production of progesterone by placenta. Progesterone treatment significantly reduced the secretion of inflammatory cytokines in serum, macrophages, and trophoblasts of B. abortus–infected mice, leading to decreased placentitis and enhancing the pup viability. Mechanistically, this decreased inflammatory response results from inhibition of NF-kB activation by progesterone. Moreover, progesterone treatment suppresses B. abortus growth within trophoblasts associated with an inability of bacteria to escape the late endosome compartment in vitro. Collectively, our data illustrate that progesterone treatment might be useful therapeutically in protection against placentitis or abortion caused by B. abortus infection.
Ergot alkaloids reduce circulating serotonin in the bovine
Authors: Valente Eriton E L , Klotz James L , Ahn Gyuchul , McLeod Kyle R , Herzing Hannah M , King Mindy , Harmon David L
Published: November 2020

Ergot alkaloids can interact with several serotonin (5-hydroxytryptamine [5-HT]) receptors provoking many physiological responses. However, it is unknown whether ergot alkaloid consumption influences 5-HT or its metabolites. Thus, two experiments were performed to evaluate the effect of ergot alkaloid feeding on 5-HT metabolism. In exp. 1, 12 Holstein steers (260 ± 3 kg body weight [BW]) were used in a completely randomized design. The treatments were the dietary concentration of ergovaline: 0, 0.862, and 1.282 mg/kg of diet. The steers were fed ad libitum, kept in light and temperature cycles mimicking the summer, and had blood sampled before and 15 d after receiving the treatments. The consumption of ergot alkaloids provoked a linear decrease (P = 0.004) in serum 5-HT. However, serum 5-hydroxytryptophan and 5-hydroxyindoleacetic acid did not change (P > 0.05) between treatments. In exp. 2, four ruminally cannulated Holstein steers (318 ± 3 kg BW) were used in a 4 × 4 Latin square design to examine the difference between seed sources on 5-HT metabolism. Treatments were: control—tall fescue seeds free of ergovaline, KY 32 seeds (L42-16-2K32); 5Way—endophyte-infected seeds, 5 way (L152-11-1739); KY31—endophyte-infected seeds, KY 31 (M164-16-SOS); and Millennium—endophyte-infected seeds, 3rd Millennium (L108-11-76). The endophyte-infected seed treatments were all adjusted to provide an ergovaline dosage of 15 μg/kg BW. The basal diet provided 1.5-fold the net energy requirement for maintenance. The seed treatments were dosed directly into the rumen before feeding. The experiment lasted 84 d and was divided into four periods. In each period, the steers received seeds for 7 d followed by a 14-d washout. Blood samples were collected on day 0 (baseline) and day 7 for evaluating the treatment response in each period. A 24 h urine collection was performed on day 7. Similar to exp. 1, serum 5-HT decreased (P = 0.008) with the consumption of all endophyte-infected seed treatments. However, there was no difference (P > 0.05) between the infected seeds. The urinary excretion of 5-hydroxyindoleacetic acid in the urine was not affected (P > 0.05) by the presence of ergot alkaloids. In conclusion, the consumption of ergot alkaloids decreases serum 5-HT with no difference between the source of endophyte-infected seeds in the bovine.
Determination of Ketamine and Norketamine in Hair and Evaluation of Polydrug Use in Ketamine Abusers Using Hair Analysis in Korea
Authors: Rhee Jongsook , Lee Juseon , Kim Jihyun , Jang Moonhee , Shin Ilchung , Lee Sangki
Published: November 2020

This study evaluated hair samples from 28 subjects who tested positive for ketamine at Seoul Institute National Forensic Service in Korea between 2016 and 2017. Ketamine in the hair was extracted using a solution of 1% hydrochloric acid in methanol for 16 h. Extracts were analyzed using gas chromatography–mass spectrometry (GC–MS) or liquid chromatography–tandem mass spectrometry (LC–MS-MS). The LC–MS-MS method was validated by determining the limit of detection (LOD), limit of quantification (LOQ), linearity, intra- and inter-accuracy, precision and matrix effect. In 59 ketamine-positive hair or hair segments from 28 ketamine abusers, the ketamine concentration was found to be in the range of 0.011–335.8 ng/mg (mean, 13.6; median, 1.8), and the norketamine concentration was found to be in the range of 0.001–35.7 ng/mg (mean, 7.5; median, 0.44). The ratio of norketamine to ketamine concentrations in hair was in the range of 0.01–1.46 (mean, 0.34; median, 0.26). The distribution of ketamine concentration in hair samples was as follows: 0.01–0.1 ng/mg in 11 samples (18.6%), 0.1–5 ng/mg in 33 samples (55.9%), 5–10 ng/mg in 4 samples (6.8%), 10–15 ng/mg in 2 samples (3.4%), 15–20 ng/mg in 4 samples (6.8%), 40–45 ng/mg in 2 samples (3.4%), 45–50 ng/mg in 1 sample (1.7%) and >100 ng/mg in only 2 samples (3.4%). In the hair of ketamine abusers, 26 of 28 subjects were detected simultaneously ketamine with other drugs, including methylenedioxymethamphetamine (MDMA; n = 9), methamphetamine (MA; n = 3), MDMA/MA (n = 3), MDMA/para-methoxyamphetamine (PMA; n = 3), MDMA/PMA/MA (n = 2), cocaine (n = 1) and other drugs (n = 5, propofol, zolpidem or benzodiazepines). Along with ketamine, other controlled drugs were detected in most of the hair samples: MDMA (60.7%), MA (28.6%), PMA (17.9%), zolpidem (17.9%) and propofol (14.3%) in the frequency of abuse. In conclusion, most of the ketamine abusers (92.9%) were polydrug abusers, who were concomitantly abusing other controlled substances.
Solving Highly Cyclic Distributed Optimization Problems Without Busting the Bank: A Decimation-based Approach
Authors: Cerquides Jesús , Rodríguez-Aguilar Juan Antonio , Emonet Rémi , Picard Gauthier
Published: November 2020

In the context of solving large distributed constraint optimization problems, belief-propagation and incomplete inference algorithms are candidates of choice. However, in general, when the problem structure is very cyclic, these solution methods suffer from bad performance, due to non-convergence and many exchanged messages. As to improve performances of the MaxSum inference algorithm when solving cyclic constraint optimization problems, we propose here to take inspiration from the belief-propagation-guided decimation used to solve sparse random graphs ($k$-satisfiability). We propose the novel DeciMaxSum method, which is parameterized in terms of policies to decide when to trigger decimation, which variables to decimate and which values to assign to decimated variables. Based on an empirical evaluation on a classical constraint optimization benchmarks (graph coloring, random graph and Ising model), some of these combinations of policies, using periodic decimation, cycle detection-based decimation, parallel and non parallel decimation, random or deterministic variable selection and deterministic or random sampling for value selection, outperform state-of-the-art competitors in many settings.
Radiation effects on wild medaka around Fukushima Dai-ichi Nuclear Power Plant assessed by micronucleus assay
Authors: Maruyama Kouichi , Wang Bing , Doi Kazutaka , Ishibashi Koji , Ichikawa San’ei , Furuhata Yoshiaki , Kubota Masahide , Watanabe Yoshito
Published: November 2020

Since the Fukushima Dai-ichi Nuclear Power Plant (F1-NPP) accident in 2011, radiation effects on wildlife in the contaminated areas have been a major concern. The outskirts of the F1-NPP are mainly rural areas, where many rice fields, streams and reservoirs are located. We searched for wild medaka (small aquarium fish) around the F1-NPP and found two wild medaka habitats (S1 and S2). S1 is a stream located 4 km from the F1-NPP, where the ambient dose equivalent rate was 0.4–0.9 μSv/h (2013–14), and S2 is a reservoir located 7.5 km from the F1-NPP, where the ambient dose equivalent rate was 9.8–22 μSv/h (2013–14 and 2017–18). Dosimeters were placed for one day at the locations where the medaka were captured, and the absorbed dose rates were estimated. Radiation effects on wild medaka were examined using micronucleus assay between 2013 and 2018. No significant difference in frequency of micronucleated gill cells was observed among the wild medaka from S1, S2 and our cultivated medaka that were used as a control.
The use of radiolucent (carbon fibre-reinforced polymer) pedicle screw fixation for serial monitoring of clear cell meningioma: a case report
Authors: Ho Daniel , Phan Thanh Ha Thy , Mobbs Ralph Jasper , Reddy Rajesh
Published: November 2020

Clear cell meningioma is an uncommon variant of meningioma associated with high rates of local recurrence and metastasis. However, monitoring for local recurrence may be complicated by metal-related artefacts generated by spinal instrumentation. We present a patient with recurrent lumbar atypical clear cell meningioma, which had been resected multiple times throughout her adolescence. Due to extensive bone and ligament resection, posterior stabilization of the lumbar spine with pedicle screws was required. To ensure clear postoperative visualization of the spinal cord for local recurrence, a carbon fibre/polyetheretherketone (CF/PEEK) pedicle screw and rod construct was used. CF/PEEK has non-inferior biomechanical and biocompatible properties to titanium, with a clear advantage of radiolucency to assist in detecting the local recurrence early and facilitating accurate radiotherapy planning.
Risk of latent and active tuberculosis infection in travellers: a systematic review and meta-analysis
Authors: Diefenbach-Elstob Tanya R , Alabdulkarim Balqis , Deb-Rinker Paromita , Pernica Jeffrey M , Schwarzer Guido , Menzies Dick , Shrier Ian , Schwartzman Kevin , Greenaway Christina
Published: November 2020

Introduction

Achieving tuberculosis (TB) elimination in low TB incidence countries requires identification and treatment of individuals at risk for latent TB infection (LTBI). Persons travelling to high TB incidence countries are potentially at risk for TB exposure. This systematic review and meta-analysis estimates incident LTBI and active TB among individuals travelling from low to higher TB incidence countries.

Methods

Five electronic databases were searched from inception to 18 February 2020. We identified incident LTBI and active TB among individuals travelling from low (<10 cases/100 000 population) to intermediate (10–100/100 000) or high (>100/100 000) TB incidence countries. We conducted a meta-analysis and meta-regression using a random effects model of log-transformed proportions (cumulative incidence). Subgroup analyses investigated the impact of travel duration, travel purpose and TB incidence in the destination country.

Results

Our search identified 799 studies, 120 underwent full-text review, and 10 studies were included. These studies included 1 154 673 travellers observed between 1994 and 2013, comprising 443 health care workers (HCW), 1 068 636 military personnel and 85 594 general travellers/volunteers. We did not identify any studies that estimated incidence of LTBI or active TB among people travelling to visit friends and relatives (VFRs). The overall cumulative incidence of LTBI was 2.3%, with considerable heterogeneity. Among individuals travelling for a mean/median of up to 6 months, HCWs had the highest cumulative incidence of LTBI (4.3%), whereas the risk was lower for military (2.5%) and general travellers/volunteers (1.6%). Meta-regression did not identify a difference in incident LTBI based on travel duration and TB incidence in the destination country. Five studies reported cases of active TB, with an overall pooled estimate of 120.7 cases per 100 000 travellers.

Conclusions

We found that travelling HCWs were at highest risk of developing LTBI. Individual risk activities and travel purpose were most associated with risk of TB infection acquired during travel.
Low-redshift quasars in the SDSS Stripe 82 – II. Associated companion galaxies and signature of star formation
Authors: Stone M B , Bettoni D , Falomo R , Kotilainen J K , Karhunen K , Paiano S , Scarpa R
Published: November 2020

We present optical spectroscopy of the close companions of 22 low-redshift (z < 0.5) quasars (QSO) selected from a larger sample of QSO in the SDSS Stripe82 region for which both the host galaxy and the large-scale environments have been investigated in our previous work. The new observations extend the number of QSO studied in our previous paper on close companion galaxies of 12 quasars. Our analysis here covers all 34 quasars from both this work and the previously published paper. We find that half of them (15 QSO; ∼44 per cent) have at least one associated galaxy. Many (12 galaxies; ∼67 per cent) of the associated companions exhibit [O ii] 3727 Å emission line as signature of recent star formation. The star formation rate (SFR) of these galaxies is modest (median SFR ∼ 4.3 M⊙ yr−1). For eight QSO, we are also able to detect the starlight of the host galaxy from which three have a typical spectrum of a post-starburst galaxy. Our results suggest that quasars do not have a strong influence on the star formation of their companion galaxies.
Modelling X-ray RMS spectra II: the ultrafast outflow of PDS 456
Authors: Härer L , Parker M L , Joyce A , Igo Z , Alston W N , Fürst F , Lobban A P , Matzeu G A , Reeves J N
Published: November 2020

We present an improved model for excess variance spectra describing ultrafast outflows and successfully apply it to the luminous ($L_{\rm bol}\sim 10^{47}\mathrm{erg}\, \mathrm{s}^{-1}$) low-redshift (z = 0.184) quasar Pico del Dias Survey (PDS) 456. The model is able to account well for the broadening of the spike-like features of these outflows in the excess variance spectrum of PDS 456, by considering two effects: a correlation between the outflow velocity and the logarithmic X-ray flux and intrinsic Doppler broadening with $v_\mathrm{int} = 10^4\, \mathrm{km}\, \mathrm{s}^{-1}$. The models were generated by calculating the fractional excess variance of count spectra from a Monte Carlo simulation. We find evidence that the outflow in PDS 456 is structured, i.e. there exist two or more layers with outflow velocities $0.27\!-\!0.30\, c$, $0.41\!-\!0.49\, c$, and $0.15\!-\!0.20\, c$ for a possible third layer, which agrees well with the literature. We discuss the prospects of generally applicable models for excess variance spectra for detecting ultrafast outflows and investigating their structure. We provide an estimate for the strength of the correlation between the outflow velocity and the logarithmic X-ray flux and investigate its validity.
Three-dimensional modelling of accretion columns: spatial asymmetry and self-consistent simulations
Authors: Gornostaev M I
Published: November 2020

The paper presents the results of three-dimensional (3D) modelling of the structure and the emission of accretion columns formed above the surface of accreting strongly magnetized neutron stars under the circumstances when a pressure of the photons generated in the column base is enough to determine the dynamics of the plasma flow. On the foundation of numerical radiation hydrodynamic simulations, several 3D models of accretion column are constructed. The first group of the models contains spatially 3D columns. The corresponding calculations lead to the distributions of the radiation flux over the sidewalls of the columns which are not characterized by axial symmetry. The second group includes the self-consistent modelling of spectral radiative transfer and two-dimensional spatial structure of the column, with both thermal and bulk Comptonization taken into account. The changes in the structure of the column and the shape of X-ray continuum are investigated depending on physical parameters of the model.
Plasmonic evolution of atomically size-selected Au clusters by electron energy loss spectrum
Authors: Lu Siqi , Xie Lin , Lai Kang , Chen Runkun , Cao Lu , Hu Kuojuei , Wang Xuefeng , Han Jinsen , Wan Xiangang , Wan Jianguo , Dai Qing , Song Fengqi , He Jiaqing , Dai Jiayu , Chen Jianing , Wang Zhenlin , Wang Guanghou
Published: November 2020

The plasmonic response of gold clusters with atom number (N) = 100–70 000 was investigated using scanning transmission electron microscopy-electron energy loss spectroscopy. For decreasing N, the bulk plasmon remains unchanged above N = 887 but then disappears, while the surface plasmon firstly redshifts from 2.4 to 2.3 eV above N = 887 before blueshifting towards 2.6 eV down to N = 300, and finally splitting into three fine features. The surface plasmon's excitation ratio is found to follow N0.669, which is essentially R2. An atomically precise evolution picture of plasmon physics is thus demonstrated according to three regimes: classical plasmon (N = 887–70 000), quantum confinement corrected plasmon (N = 300–887) and molecule related plasmon (N < 300).
The Resurrection of Interdisciplinary Pain Rehabilitation: Outcomes Across a Veterans Affairs Collaborative
Authors: Murphy Jennifer L , Palyo Sarah A , Schmidt Zachary S , Hollrah Lauren N , Banou Evangelia , Van Keuren Cynthia P , Strigo Irina A
Published: November 2020

Objective

Despite empirical support for interdisciplinary pain rehabilitation programs improving functioning and quality of life, access to this treatment approach has decreased dramatically over the last 20 years within the United States but has grown significantly in the Department of Veterans Affairs (VA). Between 2009 and 2019, VA pain rehabilitation programs accredited by the Commission on Accreditation of Rehabilitation Facilities increased 10-fold in the VA, expanding from two to 20. The aim of this collaborative observational evaluation was to examine patient outcomes across a subset of six programs at five sites.

Methods

Outcomes were assessed using agreed-upon measures of patient-reported pain intensity, pain interference across various domains, pain catastrophizing, and sleep.

Results

A total of 931 patients enrolled in the selected VA interdisciplinary pain programs, with 84.1% of participants completing the full course of treatment. Overall, all programs showed significant improvements from pretreatment to posttreatment in nearly all patient-reported outcomes. The effect sizes ranged from medium to large. Notably, the results demonstrate that positive outcomes were typical despite differences in structure and resources across programs.

Conclusions

The adverse impacts of opioid use have highlighted the importance of chronic pain treatment approaches that emphasize team-based care focused on functional improvements. This study represents the first and largest analysis of outcomes across chronic pain rehabilitation programs and demonstrates the need for increased access to similar comprehensive approaches to pain management across the health care system. Further, it suggests that a variety of structures may be effective, encouraging flexibility in adopting this interdisciplinary approach.
The influence of dietary energy and zinc source and concentration on performance, trace mineral status, and gene expression of beef steers
Authors: Carmichael-Wyatt Remy N , Genther-Schroeder Olivia N , Hansen Stephanie L
Published: November 2020

The objective of this study was to determine the effects of increased supplemental Zn from differing sources on growth performance of steers fed diets differing in net energy. Angus steers (n = 72, 324 ± 2.1 kg) with Genemax gain scores of 3, 4, or 5 were blocked by BW and stratified by Genemax gain score into 12 pens of 6 steers each for 158 d. Pens were randomly assigned to 1 of 3 Zn treatments (ZNTRT): 1) control (no supplemental Zn, analyzed 33 mg Zn/kg DM; CON); 2) inorganic Zn (CON + 120 mg supplemental Zn/kg DM as ZnSO4 for entire trial; INZN); or 3) 120 mg supplemental Zn/kg DM as Zn-amino acid complex (Availa-Zn; Zinpro, Eden Prairie, MN) for first 60 d, then a blend of ZnSO4 and Zn-AA complex (CON + 60 mg supplemental Zn/kg DM as ZnSO4 + 60 mg supplemental Zn/kg DM as Zn-amino acid complex) for the remainder of the trial (ZNBLD). Two dietary energy strategies (ENERGY) were formulated to reach ADG rates of 1) 1.6 kg/d (LE) or 2) 2.0 kg/d (HE) utilizing a 3 × 2 factorial arrangement (12 steers/treatment). All steers were fed LE for a 60 d growing period, then pens were randomly assigned to ENERGY treatments fed the remaining 91 d. Day 60 BW tended to be greater (P = 0.07) in steers receiving supplemental Zn vs. CON. Liver Cu was decreased in Zn supplemented steers vs. CON (P = 0.02). Liver Zn concentrations on d 56 did not differ for Zn vs. CON (P = 0.22) nor were there differences due to Zn source (P = 0.98). There were or tended to be ZNTRT × ENERGY effects for d 67–90 ADG and G:F (P ≤ 0.01), and d 122 BW and d 90–122 G:F (P ≤ 0.10) driven by improved performance for ZNBLD-HE over ZNBLD-LE, while ENERGY within CON and INZN did not differ. Day 90–122 ADG, overall ADG and overall G:F was greater (P ≤ 0.02) and d 67–90 G:F tended to be greater (P = 0.10) for HE vs. LE. No ZNTRT × ENERGY or ZNTRT effects were detected for HCW, REA, BF, KPH, MS, or YG (P ≥ 0.37) while HE increased HCW, BF, MS, and YG compared with LE (P ≤ 0.05). In the liver, ZNTRT affected d 97 MT1A expression (P = 0.03) where INZN was greater than ZNBLD or CON (P ≤ 0.02), while ZIP14 was unaffected due to ZNTRT, ENERGY, or the interaction (P ≥ 0.39). Supplying supplemental Zn as ZNBLD during the transition period appeared to improve performance measures, but no final performance advantages were noted due to increased supplemental Zn, regardless of source. Additionally, differences in liver MT1A expression may indicate differing post-absorptive metabolism between Zn sources.
Silver nanoparticles achieve cytotoxicity against breast cancer by regulating long-chain noncoding RNA XLOC_006390-mediated pathway
Authors: Tao Lin , Chen Xi , Sun Jiawei , Wu Changjun
Published: November 2020

The specific cytotoxic effect of nanoparticles on tumor cells may be used in future antitumor clinical applications. Silver nanoparticles (AgNPs) have been reported to have potent cytotoxic effect, but the mechanism is unclear. Here, AgNPs were synthesized, and the particle average size was 63.1 ± 8.3 nm and showed a nearly circular shape, which were determined by transmission electron microscopy and field emission scanning electron microscopy. The selected area electron diffraction patterns showed that the nanoparticles were crystalline. The energy-dispersive X-ray spectrum proved that silver is the main component of nanoparticles. The AgNPs showed potent cytotoxicity in breast cancer cells, no matter whether they were tamoxifen sensitive or resistant. Next, we found that a long noncoding RNA, XLOC_006390, was decreased in AgNPs-treated breast cancer cells, coupled to inhibited cell proliferation, altered cell cycle and apoptotic phenotype. Downstream of AgNPs, XLOC_006390 was recognized to target miR-338-3p and modulate the SOX4 expression. This signaling pathway also mediates the AgNPs function of sensitizing tamoxifen-resistant breast cancer cells to tamoxifen. These results provide a new clue for the antitumor mechanism of AgNPs, and a new way for drug development by using AgNPs.
Association of the transthyretin variant V122I with polyneuropathy among individuals of African descent
Authors: Parker Margaret M. , Damrauer Scott M. , Tcheandjieu Catherine , Erbe David , Aldinc Emre , Hawkins Philip N. , Gillmore Julian D. , Hull Leland E. , Lynch Julie A. , Joseph Jacob , Ticau Simina , Flynn-Carroll Alexander O. , Deaton Aimee M. , Ward Lucas D. , Assimes Themistocles L. , Tsao Philip S. , Chang Kyong-Mi , Rader Daniel J. , Fitzgerald Kevin , Vaishnaw Akshay K. , Hinkle Gregory , Nioi Paul
Published: November 2020

Introduction

Hereditary transthyretin-mediated (hATTR) amyloidosis is an underdiagnosed, progressively debilitating disease caused by mutations in the transthyretin (TTR) gene. The V122I variant, one of the most common pathogenic TTR mutations, is found in 3-4% of Black individuals, and has been associated with cardiomyopathy.

Methods

To better understand the phenotypic consequences of carrying V122I, we conducted a phenome-wide association study scanning 427 ICD diagnosis codes for association with this variant in Black participants of the UK Biobank (n= 6,062). Significant associations were tested for replication in the Penn Medicine Biobank (n= 5,737) and the Million Veteran Program (n= 82,382).

Results

Our analyses discovered a significant association between V122I and polyneuropathy diagnosis (odds ratio = 6.4, 95% confidence interval [CI] = 2.6 to 15.6, P = 4.2 × 10−5) in the UK Biobank,which was replicated in the Penn Medicine Biobank (p=6.0×10−3)) and Million Veteran Program (P= 1.8×10−4)). Polyneuropathy prevalence among V122I carriers was 2.1–9.0% across biobanks. The cumulative incidence of common hATTR amyloidosis manifestations (carpal tunnel syndrome, polyneuropathy, cardiomyopathy, heart failure) was significantly enriched in V122I carriers versus non-carriers (hazard ratio = 2.8, 95% CI = 1.7–4.5, P = 2.6 × 10−5) in the UK Biobank;37.4% of V122I carriers having a diagnosis of any one of these manifestations by age 75.

Conclusions

Our findings show that, although the V122I variant is known to be associated with cardiomyopathy, carriers are also at significantly increased risk of developing polyneuropathy. These results also emphasize the underdiagnosis of disease in V122I carriers with a significant proportion of subjects showing phenotypic changes consistent with hATTR. Greater understanding of the manifestations associated with V122I is critical for earlier diagnosis and treatment.