bluuebunny/crossref_metadata_2025 huggingface dataset:
Inland mariculture of reef corals amenable for the ornamental trade
Authors: Rinkevich Baruch , Hunter Cynthia
Published: March 2022

The worldwide market for ornamental saltwater invertebrates supplies the needs of millions of aquarium hobbyists, public exhibitions (i.e., zoos) universities and research institutions. With respect to reef building corals, it is estimated that more than half a million coral colonies/year from a total 93 genera, were exported globally during the period of 1985-1997. International value of retail sale of live coral trade alone is estimated as $78 million in 1997 (not including the illegally, widely smuggled material). The continuous, large-scale collection of marine organisms is responsible, in many places, for the destruction of coral reefs. The expected expansion of the trade further threatens these fragile habitats. While no true captive-bred corals are commercially available, our long-term goal is to develop ex situ inland farming of coral colonies that will circumvent the need for in situ collections and will provide domesticated specimens for the trade and for research. We simultaneously studied two model branching coral species, Stylophora pistillata (Pocilloporidae; in Israel) and Porites (Poritidae; in the US). The proposal included three specific aims: (a) To develop protocols for nubbins (small fragments, down to the size of a single polyp) usage in coral farming;(b) To address the significance of colony pattern formation to the coral trade; and (c) To develop the protocols of using nubbins in physiological and ecotoxicological assays (using oil dispersants, the expression of the stress protein HSP-70, household detergents, etc.). Ten scientific publications (published manuscripts, accepted for publications, submitted to scientific journals, in preparation), revealing results that were related to all three specific aims, originated from this BARD proposal. As a result of the work supported by the BARD, we have now, in hand, original and improved protocols for coral maintenance ex situ, proven expertise on manipulating coral colonies’ pattern formation and biological knowledge on island mariculture of reef corals (from Hawaii and from the Red Sea) amenable for the ornamental trade (for public and private aquaria use, for experimentation). At least one Israeli company (Red Sea Corals, Ltd., KibbutzSaar) is using our methodologies for further developing this new mariculture sector. We are now in the process of introducing the rationale and methodologies to Hawaiian private entities to expand dissemination of the research outcomes.
Sorption and Mobility of Pharmaceutical Compounds in Soils Irrigated with Treated Wastewater
Authors: Chefetz Benny , Chorover Jon
Published: March 2022

Research into the fate of pharmaceutical compounds (PCs) in the environment has focused on aspects of removal efficiency during sewage treatment, degradation in surface water and accumulation in soils and sediments. However, very little information is available on the binding interactions of pharmaceuticals with dissolved organic matter (DOM) originating from wastewater treatment. Such interactions can significantly affect the transport potential of PCs in soils by altering compound affinity for soil particle surfaces. Our primary hypothesis is that the transport potential of PCs in soils is strongly impacted by the type and strength of interaction with DOM and the stability of resulting DOM-PC complexes. The overarching goal of the proposed work is to develop a better understanding of the risk associated with introduction of PCs into the environment with treated wastewater. This goal has been achieved by elucidating the mechanisms of the interaction of selected pharmaceuticals (that have shown to be widespread wastewater contaminants) with DOM constituents; by determining the stability and fate of DOM-PC complexes introduced to soils and soil constituents; and by evaluating the potential uptake of these compounds by plants. Based on the results obtained in this study (column and batch sorption-desorption experiments), we suggest that PCs can be classified as slow-mobile compounds in SOM-rich soil layers. When these compounds pass this layer and/or are introduced into SOM-poor soils, their mobility increases significantly. Our data suggest that in semiarid soils (consisting of low SOM), PCs can potentially be transported to the groundwater in fields irrigated with reclaimed wastewater. Moreover, the higher mobility of the acid PCs (i.e., naproxen and diclofenac) in freshwater column systems suggests that their residues in soils irrigated with reclaimed wastewater can leach from the root zone and be transported to the groundwater after rain events. Our data obtained from the binding experiments of PCs with DOM demonstrate that the hydrophobic DOM fractions were more efficient at sorbing PCs than the more polar hydrophilic fractions at a pH near the pKa of the analytes. At the pH of natural semiarid water and soil systems, including that of reclaimed wastewater and biosolids, the role of the hydrophobic fractions as sorption domains is less important than the contribution of the hydrophilic fractions. We also hypothesize that the DOM fractions interact with each other at the molecular level and do not act as independent sorption domains. In summary, our data collected in the BARD project demonstrate that the sorption abilities of the DOM fractions can also significantly affect the mobility of pharmaceutical compounds in soils influenced by intensive irrigation with treated wastewater or amended with biosolids.
Avian Influenza Virus H9N2: Characterization and Control Strategies
Authors: Perk Shimon , Garcia Maricarmen , Panshin Alexander , Banet-Noach Caroline , Gissin Irina , Jackwood Mark W. , Stallknecht David
Published: March 2022

Control of Avian Influenza (AI) infection is a highly topical subject of major economicimportance for the worldwide poultry industry at the national level and for international trade.H9N2 viruses are endemic in poultry throughout Asia and the Middle East, causing major losses inproduction. Moreover, these viruses pose wider threats since they have been isolated from bothswine and humans. At the same time, study of the AI viruses affords an opportunity to explore anumber of problems of intriguing scientific interest. The overall goal of this project was to developa sound control strategy for avian influenza subtype H9N2 viruses (AI H9N2) in commercialpoultry in Israel. The one-year feasibility study focused on two main goals, namely: to study themolecular characteristics of AI H9N2 circulating during the last seven years in Israel and todevelop tools enabling differentiation between the immune response to vaccination and infectionwith H9N2.Genetic and phylogenetic characterization of 29 selected AI H9N2 isolates (2000-2006)was performed by complete sequencing of hemagglutinin (HA), neuraminidase (NA), and all sixinternal genes [nucleoprotein (NP), polymerase basic 1 (PB1), polymerase basic 2 (PB2),polymerase acid (PA), matrix (M), and nonstructural (NS) genes]; comparative phylogenetic andgenetic analyses of these sequences; and comparative genetic analyses of deduced amino acidsequences of the HA, NA, NS1, and NS2 proteins. The major conclusions of the molecularanalyses were: (1) Israeli isolates, together with other H9N2 viruses isolated in Middle Eastcountries, comprise a single regional sublineage related to the G1-lineage. In addition, Israeliisolates subdivided into three different subgroups. Genetic analysis of these viruses suggests thatthey underwent divergent evolution paths.
Learning from Nature How to Design Anti-insect Selective Pesticides - Clarification of the Interacting Face between Insecticidal Toxins and their Na-channel Receptors
Authors: Gurevitz Michael , Catterall William A. , Gordon Dalia
Published: March 2022

Structural details on the interacting faces of toxins and sodium channels (Navs), and particularly identification of elements that confer specificity for insects, are difficult to approach and require suitable experimental systems. Therefore, natural toxins capable of differential recognition of insect and mammalian Navs are valuable leads for design of selective compounds in insect control. We have characterized several scorpion toxins that vary in preference for insect and mammalian Navs, and identified residues important for their action. However, despite many efforts worldwide, only little is known about the receptor sites of these toxins, and particularly on differences between these sites on insect and mammalian Navs. Another problem arises from the massive overuse of chemical insecticides, which increases resistance buildup among various insect pests. A possible solution to this problem is to combine different insecticidal compounds, especially those that provide synergic effects. Our recent finding that combinations of insecticidal receptor site-3 toxins (sea anemone and scorpion alpha) with scorpion beta toxins or their truncated derivatives are synergic in toxicity to insects is therefore timely and strongly supports this approach. Our ability to produce toxins and various Navs in recombinant forms, enable thorough analysis and structural manipulations of both toxins and receptors. On this basis we propose to (1) restrict by mutagenesis the activity of insecticidal scorpion -toxins and sea anemone toxins to insects, and clarify the molecular basis of their synergic toxicity with antiinsect selective -toxins; (2) identify Nav elements that interact with scorpion alpha and sea anemone toxins and those that determine toxin selectivity to insects; (3) determine toxin-channel pairwise side-chain interactions by thermodynamic mutant cycle analysis using our large collection of mutant -toxins and Nav mutants identified in aim 2; (4) clarify the mode of interaction of truncated -toxins with insect Navs, and elucidate how they enhance the activity of insecticidal site-3 toxins. This research may lead to rational design of novel anti-insect peptidomimetics with minimal impact on human health and the environment, and will establish the grounds for a new strategy in insect pest control, whereby a combination of allosterically interacting compounds increase insecticidal action and reduce risks of resistance buildup.
Integrated Studies of Chloroplast Ribonucleases
Authors: Schuster Gadi , Stern David
Published: March 2022

Gene regulation at the RNA level encompasses multiple mechanisms in prokaryotes and eukaryotes, including splicing, editing, endo- and exonucleolytic cleavage, and various phenomena related to small or interfering RNAs. Ribonucleases are key players in nearly all of these post-transcriptional mechanisms, as the catalytic agents. This proposal continued BARD-funded research into ribonuclease activities in the chloroplast, where RNase mutation or deficiency can cause metabolic defects and is often associated with plant chlorosis, embryo or seedling lethality, and/or failure to tolerate nutrient stress. The first objective of this proposal was to examined a series of point mutations in the PNPase enzyme of Arabidopsis both in vivo and in vitro. This goal is related to structure-function analysis of an enzyme whose importance in many cellular processes in prokaryotes and eukaryotes has only begun to be uncovered. PNPase substrates are mostly generated by endonucleolytic cleavages for which the catalytic enzymes remain poorly described. The second objective of the proposal was to examine two candidate enzymes, RNase E and RNase J. RNase E is well-described in bacteria but its function in plants was still unknown. We hypothesized it catalyzes endonucleolytic cleavages in both RNA maturation and decay. RNase J was recently discovered in bacteria but like RNase E, its function in plants had yet to be explored.   The results of this work are described in the scientific manuscripts attached to this report. We have completed the first objective of characterizing in detail TILLING mutants of PNPase Arabidopsis plants and in parallel introducing the same amino acids changes in the protein and characterize the properties of the modified proteins in vitro. This study defined the roles for both RNase PH core domains in polyadenylation, RNA 3’-end maturation and intron degradation. The results are described in the collaborative scientific manuscript (Germain et al 2011). The second part of the project aimed at the characterization of the two endoribonucleases, RNase E and RNase J, also in this case, in vivo and in vitro. Our results described the limited role of RNase E as compared to the pronounced one of RNase J in the elimination of antisense transcripts in the chloroplast (Schein et al 2008; Sharwood et al 2011). In addition, we characterized polyadenylation in the chloroplast of the green alga Chlamydomonas reinhardtii, and in Arabidopsis (Zimmer et al 2009).   Our long term collaboration enabling in vivo and in vitro analysis, capturing the expertise of the two collaborating laboratories, has resulted in a biologically significant correlation of biochemical and in planta results for conserved and indispensable ribonucleases. These new insights into chloroplast gene regulation will ultimately support plant improvement for agriculture.
Functional genomics of reproduction and division of labor in a key non-Apis pollinator
Authors: Bloch Guy , Robinson Gene E. , Band Mark
Published: March 2022

i. List the original objectives, as defined in the approved proposal, and any revisions made at the beginning or during the course of project. Our objectives were: 1) develop state-of-the-art functional genomics tools for B. terrestris. These resources will be then used to: 2) characterize genes and molecular pathways that are associated with reproduction, 3) characterize genes and molecular pathways associated with specialization in foraging or nursing activities, and 4) determine the extent to which juvenile hormone (JH) is involved in the regulation of reproduction and division of labor. 5) Use RNA interference to down regulate genes associated with reproductive physiology, division of labor, or both. A decrease in the cost of RNA sequencing enabled us to further use the BARD support to extend our research to three additional related projects: A) The regulation of body size which is crucial for understanding both reproduction (castedetermination) and (size based) division of labor in bumblebees. B) Analyze RNA editing in our RNA sequencing data which improves the molecular understanding of the systems we study. C) The influence of JH on the fat body in addition to the brain on which we focused in our proposal. The fat body is a key tissue regulating insect reproduction and health.  ii. Background to the topic. Bees are by far the most important pollinators in agricultural and natural ecosystems. The recent collapse of honey bee populations, together with declines in wild bee (including bumble bee) populations, puts their vital pollination services under severe threat. A promising strategy for circumventing this risk is the domestication and mass-rearing of non-Apis bees. This approach has been successfully implemented for several bumble bees including Bombusterrestris in Israel, and B. impatiens in the US, which are mass-reared in captivity. In spite of their critical economic and environmental value, little is known about the physiology and molecular biology of bumble bees. In this collaborative project we developed functional genomics tools for the bumble bee B. terrestris and use these tools for a first thorough study on the physiology and molecular biology of reproduction, dominance, and division of labor in a bumble bee.  iii. Major conclusions, solutions. The valuable molecular data of this project together with the functional tools and molecular information generated in this BARD funded project significantly advanced the understanding of bumblebee biology which is essential for maintaining their vital pollination services for US and Israel agriculture.
Characterization and Control Strategies of Low Pathogenic Avian Influenza Virus H9N2
Authors: Perk Simon , Mundt Egbert , Panshin Alexander , Davidson Irit , Shkoda Irina , AlTori Ameera , Garcia Maricarmen
Published: March 2022

The avian influenza virus, subtype H9N2 subtype, defined as having a low pathogenicity, causes extensive economical losses in commercial flocks, probably due to management and synergism with other pathogens. AIV H9N2 was first identified in Israel in the year 2000, and since then it became endemic and widespread in Israel. Control by vaccination of commercial flocks with an inactivated vaccine has been introduced since 2007. In face of the continuous H9N2 outbreaks, and the application of the vaccination policy, we aimed in the present study to provide a method of differentiating naturally infected from vaccinated animals (DIVA). The aim of the assay would be detect only antibodies created by a de-novo infection, since the inactivated vaccine virus is not reproducing, and might provide a simple tool for mass detection of novel infections of commercial flocks. To fulfill the overall aim, the project was designed to include four operational objectives: 1. Evaluation of the genetic evolution of AIV in Israel; 2. Assessment of the diagnostic value of an NS1 ELISA; 3. NS1 ELISA as evaluation criteria for measuring the efficacy of vaccination against H9N2 AIV; 4. Development of an AIV H9 subtype specific ELISA systems.  Major conclusion and implications drawn from the project were: 1. A continuous genetic change occurred in the collection of H9N2 isolates, and new introductions were identified. It was shown thatthe differences between the HA proteins of viruses used for vaccine productionand local fieldisolatesincreasedin parallelwith the durationand intensity ofvaccine use, therefore, developing a differential assay for the vaccine and the wild type viruses was the project main aim.  2. To assess the diagnostic value of an NS1 ELISA we first performed experimental infection trials using representative viruses of all introductions, and used the sera and recombinant NS1 antigens of the same viruses in homologous and heterologous NS1 ELISA combination. The NS1 ELISA was evidently reactive in all combinations, and did not discriminate significantly between different groups.   3. However, several major drawbacks of the NS1 ELISA were recognized: a) The evaluation of the vaccination effect in challenged birds, showed that the level of the NS1 antibodies dropped due to the vaccination-dependent virus level drop; b) the applicability of the NS1-ELISA was verified on sera of commercial flocks and found to be unusable due to physico-chemical composition of the sera and the recombinant antigen, c) commercial sera showed non-reactivity that might be caused by many factors, including vaccination, uncertainty regarding the infection time, and possibly low antigen avidity, d) NS1 elevated antibody levels for less than 2 months in SPF chicks. Due to the above mentioned reasons we do not recommend the application of the DIVA NS1 ELISA assay for monitoring and differentiation AIV H9N2 naturally-infected from vaccinated commercial birds.
Nutrition of the Developing Chick Embryo: Nutrient Uptake Systems of the Yolk Sac Membrane and Embryonic Intestine
Authors: Wong Eric A. , Uni Zehava
Published: March 2022

We have examined the developmental changes in composition, amount, and uptake of yolk nutrients (fat, protein, water and carbohydrates) and the expression ofnutrient transporters in the yolk sac membrane (YSM) from embryonic day 11 (Ell) to 21 (E21) and small intestine from embryonic day 15 (E15) to E21 in embryos from young (22-25 wk) and old (45-50 wk) Cobb and Leghorn breeder flocks.   The developmental expression profiles for the peptide transporter 1 (PepTl), the amino acid transporters, EAAT3, CAT-1 and BOAT, the sodium glucose transporter (SGLTl), the fructose transporter (GLUT5), the digestive enzymes aminopeptidase N (APN) and sucraseisomaltase (SI) were assayed by the absolute quantification real time PCR method in the YSM and embryonic intestine. Different temporal patterns of expression were observed for these genes.   The effect of in ovo injection of peptides (the dipeptide Gly-Sar, purified peptides, trypsin hydrolysate) on transporter gene expression has been examined in the embryonic intestine. Injection of a partial protein hydrolysate resulted in an increase in expression of the peptide transporter PepT2.   We have initiated a transcriptome analysis of genes expressed in the YSM at different developmental ages to better understand the function of the YSM.
Interactions of engineered nanoparticles with dissolved organic matter (DOM) and organic contaminants in water
Authors: Chefetz Benny , Xing Baoshan , Chen Yona
Published: March 2022

Background: Engineered carbon nanotubes (CNTs) are expected to be increasingly released into the environment with the rapid increase in their production and use. The discharged CNTs may interact with coexisting contaminants and subsequently change environmental behaviors and ecological effects of both the CNTs themselves and the contaminants. Dissolved organic matter (DOM) plays a critical role in the transport of CNTs in the aquatic environment, affecting both CNT's surface properties through adsorption, and its colloidal stability in solution. Therefore, CNT-bound DOM complexes may interact with coexisting contaminants, thus affecting their environmental fate. With increasing production and use of CNTs, there is an increasing risk that humans could be exposed to CNTs mainly through ingestion and inhalation. Since CNTs can be carriers of contaminants due to their high adsorption affinity and capacity, the distribution of these nanoparticles in the environment holds a potential environmental and health risk. Project objectives: The overall goal of this project was to gain a better understanding of the environmental behavior of engineered nanoparticles with DOM and organic pollutant in aqueous systems. The scope of this study includes: characterizing various types of engineered nanoparticles and their interaction with DOM; binding studies of organic contaminants by nanoparticles and DOM-nanoparticle complexes; and examining interactions in DOM-nanoparticles-contaminant systems. Major conclusions, solutions and achievements: DOM has a pronounced effect on colloidal stability of CNTs in solution and on their surface chemistry and reactivity toward associated contaminants. The structure and chemical makeup of both CNTs and DOM determine their interactions and nature of formed complexes. CNTs, contaminants and DOM can co-occur in the aquatic environment. The occurrence of co-contaminants, as well as of co-introduction of DOM, was found to suppress the adsorption of organic contaminants to CNTs through both competition over adsorption sites and direct interactions in solution. Furthermore, the release of residual contaminants from CNTs could be enhanced by biomolecules found in the digestive as well as the respiratory tracts, thus increasing the bioaccessibility of adsorbed contaminants and possibly the overall toxicity of contaminant-associated CNTs. Contaminant desorption could be promoted by both solubilization and sorptive competition by biological surfactants. Scientific and agricultural implications: The information gained in the current project may assist in predicting the transport and fate of both CNTs and associated contaminants in the natural environment. Furthermore, the results imply a serious health risk from contaminant-associated CNTs.
Control of Bovine Anaplasmosis: Protective Capacity of the MSP2 Allelic Repertoire
Authors: Brayton Kelly A. , Shkap Varda , Palmer Guy H. , Brown Wendy C. , Molad Thea
Published: March 2022

Anaplasmosis is an arthropod-borne disease of cattle caused by the rickettsia Anaplasmamarginale and is an impediment to efficient production of healthy livestock in both Israel and the United States. Currently, the only effective vaccines are derived from the blood of infected cattle. The risk of widespread transmission of both known and newly emergent pathogens has prevented licensure of live blood-based vaccines in the U.S. and is a major concern for their continued use in Israel. Consequently, development of a safe, effective vaccine is a high priority.   Despite its drawbacks as a live, blood-based vaccine, the Israel vaccine strain protects against disease upon challenge with wild-type A. marginale in extensive experimental trials and during 50 years of deployment in Israel. Field studies in Australia and Argentina indicate that this protection is broadly effective. Thus, to identify antigens for development of a safe and effective recombinant vaccine, we have used a comparative genomics approach by sequencing the Israel vaccine strain and searching for shared surface antigens with sequenced wild-type U.S. strains. We have focused on Msp2, the immune-dominant but antigenically variable surface protein, based on shared structure among strains and demonstration that antibody from cattle immunized with the Israel vaccine strain binds Msp2 from the genetically and geographically distinct U.S. St. Maries strain, consistent with the ability to protect against St. Maries challenge. Importantly, we have defined the full repertoire of Msp2 simple variants encoded by the vaccine strain and hypothesize that a recombinant vaccine encoding this full repertoire will induce protection equivalent to that induced by the live vaccine strain. Any escape from immunity by generation of complex Msp2 variants is predicted to carry a severe fitness cost that prevents high-level bacteremia and disease— consistent with the type of protection induced by the live vaccine strain.   We tested the hypothesis that the Msp2 simple variant repertoires in wild-type A. marginale strains are recognized by antibody from cattle immunized with the Israel vaccine strain and that immunization with the vaccine strain Msp2 repertoire can recapitulate the protection provided by the vaccine strain upon challenge with Israel and U.S. strains of A. marginale. Our findings demonstrate that a set of conserved outer membrane proteins are recognized by immune serum from A. centrale vaccinated animals but that this set of proteins does not include Msp2. These findings suggest that “subdominant” immunogens are required for vaccine induced protection.
Genetic and cellular networks regulating cell fate at the shoot apical meristem
Authors: Eshed-Williams Leor , Zilberman Daniel
Published: March 2022

The shoot apical meristem establishes plant architecture by continuously producing new lateral organs such as leaves, axillary meristems and flowers throughout the plant life cycle. This unique capacity is achieved by a group of self-renewing pluripotent stem cells that give rise to founder cells, which can differentiate into multiple cell and tissue types in response to environmental and developmental cues. Cell fate specification at the shoot apical meristem is programmed primarily by transcription factors acting in a complex gene regulatory network. In this project we proposed to provide significant understanding of meristem maintenance and cell fate specification by studying four transcription factors acting at the meristem. Our original aim was to identify the direct target genes of WUS, STM, KNAT6 and CNA transcription factor in a genome wide scale and the manner by which they regulate their targets. Our goal was to integrate this data into a regulatory model of cell fate specification in the SAM and to identify key genes within the model for further study. We have generated transgenic plants carrying the four TF with two different tags and preformed chromatin Immunoprecipitation (ChIP) assay to identify the TF direct target genes. Due to unforeseen obstacles we have been delayed in achieving this aim but hope to accomplish it soon. Using the GR inducible system, genetic approach and transcriptome analysis [mRNA-seq] we provided a new look at meristem activity and its regulation of morphogenesis and phyllotaxy and propose a coherent framework for the role of many factors acting in meristem development and maintenance. We provided evidence for 3 different mechanisms for the regulation of WUS expression, DNA methylation, a second receptor pathway - the ERECTA receptor and the CNA TF that negatively regulates WUS expression in its own domain, the Organizing Center. We found that once the WUS expression level surpasses a certain threshold it alters cell identity at the periphery of the inflorescence meristem from floral meristem to carpel fate [FM]. When WUS expression highly elevated in the FM, the meristem turn into indeterminate. We showed that WUS activate cytokinine, inhibit auxin response and represses the genes required for root identity fate and that gradual increase in WUCHEL activity leads to gradual meristem enlargement that affect phyllotaxis. We also propose a model in which the direction of WUS domain expansion laterally or upward affects meristem structure differently. We preformed mRNA-seq on meristems with different size and structure followed by k-means clustering and identified groups of genes that are expressed in specific domains at the meristem. We will integrate this data with the ChIP-seq of the 4 TF to add another layer to the genetic network regulating meristem activity.
KORELASI NILAI DAYA DUKUNG ULTIMIT TIANG BOR HASIL ANALISIS DENGAN HASIL PENGUJIAN PDA TEST (STUDI KASUS: PEMBANGUNAN JALAN TOL BOGOR RING ROAD SEKSI III A RUAS SIMPANG YASMIN - SIMPANG SALABENDA)
Authors: Rahman Ari Awalul , Hariati Feril , Chayati Nurul , Taqwa Fadhila Muhammad Libasut
Published: March 2022

ABSTRAK

Pondasi tiang bored pile, salah satu jenis pondasi yang biasanya digunakan untuk bangunan tinggi, atau bangunan dengan beban yang besar. Penentuan suatu pondasi ditentukan dengan mencari daya dukung beban maksimum atau daya dukung izin pada pondasi serta keamanan tiang, dengan kombinasi tahanan selimut dan tahanan ujung tiang diperlukan pengujian dinamis dan statik. Tujuan dari studi ini untuk membandingkan daya dukung tiang bor hasil analisis dengan nilai daya dukung PDA test. Menggunakan metode Meyerhof, Decourt, dan Resee O’neill. Berdasarkan data hasil PDA serta dilakukannya metode analisis menggunakan data N-SPT pada pembangunan di lokasi proyek jalan Tol Bogor Ring Road Seksi III A. Hasil Root Means Square Error daya dukung statis  menggunakan metode Meyerhof  R2= 0,1946, Decourt R2= 0,0077, dan Resee O’neill R2= 0,3795  dan hasil yang mendekati pada analisis daya dukung ultimit tiang statis menggunakan teori Meyerhof dan Decourt.

ABSTRACT

Bored pile foundation, a type of foundation that is usually used for tall buildings, or buildings with large loads. Determination of a foundation is determined by finding the maximum load bearing capacity or permit bearing capacity on the foundation and pile safety, with a combination of blanket resistance and pile end resistance required dynamic and static testing. This study aims to compare the bearing capacity of the drill pile as a result of the analysis with the PDA test carrying capacity using the Meyerhof, Decourt, and Resee O'neill methods. Based on PDA results data and analysis methods using N-SPT data on construction at the Bogor Ring Road Section III toll road project site A.Results of Root Means Square Error, static carrying capacity uses the Meyerhof R2 = 0.1946 method, Decourt R2 = 0.0077 , and Resee O'neill R2 = 0.3795 and the results are close to the ultimate bearing capacity analysis of static piles using Meyerhof and Decourtanalysis.

 Keywords: bored pile, pile bearing capacity, PDA test, N-SPT data, metode Meyerhof, Decourt, and Resee O’neill.
HUBUNGAN KEHARMONISAN KELUARGA DENGAN KENAKALAN REMAJA DI PKBM AL-JAUHAR KECAMATAN BOGOR UTARA KOTA BOGOR
Authors: Safitri Ani
Published: March 2022

Keharmonisan keluarga adalah situasi atau kondisi keluarga dimana terjalinnya kasih� sayang, mempunyai waktu bersama keluarga, adanya kerjasama dalam keluarga, komunikasi setiap anggota keluarga dan minimnya ketegangan di dalam rumah. Metode yang digunakan dalam penelitian ini adalah metode deskriptif kuantitatif, dengan alat pengumpulan data, berupa angket, observasi, wawancara, dan studi pustaka. Populasi dalam penelitian ini sekaligus dijadikan sampel yaitu warga belajar Paket B di PKBM Al-Jauhar Kecamatan Bogor Utara Kota Bogor, berjumlah 30 orang. Dari hasil penelitian yang telah dilakukan, diperoleh nilai koefisien korelasi produk
momen person atau r hitung sebesar 0,92. Maka nilai r hitung sebesar 0,92 berada pada interval 0,80-1,000 dengan interpretasi Sangat Kuat dan berkontribusi sebanyak 84%. Nilai t hitung sebesar 12.167 dan ttabel sebesar 2,048 sehingga thitung> ttabel (5,365>2,048). Dengan demikian, maka penelitian ini menolak Hipotesis uji atau
Hipotesis nol (H0), dan menerima H1 yang dengan demikian, maka hubungan antara keharmonisan keluarga dengan kenakalan remaja di PKBM Al-Jauhar Kecamatan Bogor
Utara Kota Bogor.
CHARACTER BUILDING PROGRAM MELALUI KEGIATAN EDUKASI DILINGKUNGAN TAMAN KOTA BOGOR
Authors: Yusiyaka Rahmi Alendra , Nisa Jihan Solehatun , Kurniasih Indah
Published: March 2022

Kota merupakan suatu pusat pemukiman dan kegiatan penduduk yang cukup padat, memiliki permasalahan cukup kompleks, bersifat dinamis, serta memiliki pengaruh besar dalam bidang ekonomi. Adapun tujuan dari pembuatan program karakter building ini adalah menjadikan taman-taman yang ada di Kota Bogor sebagai sarana alternative untuk kegiatan edukasi bagi anak-anak. Makalah ini disusun berdasarkan studi pustaka (library research) yang menggunakan buku-buku dan literaratur-literatur lainnya sebagai objek yang utama ini menggunakan metode kajian pustaka. Keberadaan tamantaman yang ada di Kota Bogor sudah cukup banyak dan memberikan fasilitas yang baik bagi para anak-anak, akan tetapi belum mampu memberikan perubahan dan edukasi yang baik terhadap anak-anak. Akan lebih baik taman dimanfaatkan sebagai sarana wisata sekaligus pembelajaran melalui program karakter building yang dapat memudahkan tercapainya tujuan pendidikan nasional, yang berisikan salah satunya untuk mencerdaskan kehidupan bangsa
PENDIDIKAN KELUARGA RESPONSIF GENDER
Authors: Yusiyaka Rahmi Alendra , Safitri Ani
Published: March 2022

Keluarga merupakan pijakan pertama dalam perkembangan kehidupan manusia, melalui keluarga manusis mengenal berbagai pengertahuan dan dapat mengembangkan berbagai potensi yang dimiliki. Pada prinsipnya, setiap manusia secara fitrah sudah dibekali potensi baik, maka melalui keluargalah segala pontesi itu dapat distimulus untuk mewujudkan sumberdaya yang berkualitas. Pendidikan dalam keluarga yang responsif gender sangat diutamakan dalam pendidikan keluarga,dengan demikian anak bisa berkembang tanpa
adanya bias gender, yang akan sangat berpengaruh kepada polapikir dan pembentukan karakter manusia yang bebudi luhur dan berdaya, sehingga dapat menwujudkan sumberdaya manusia yang berkualitas.Orang tua merupakn pihak utama yang bertanggung jawab dalam pendidikan didalam keluargUntuk mewujudkan hal ini tentu perlu disiapkan beberapa hal, agar tujuan tersebut apat dicapai, layaknya sebuh lembaga pendidikan, ada yang perlu
dipersiapakan, dintaranya; pendidik, sarana prasarana serta lingkungan keluarga yang dapat menunjang terselenggaranya pendidikan keluarga. Namun reaitasnya, banyak sekali ditemukan orag tua yang bertindak sebagai pendidik tidak memiliki kematangan keilmuan
dan pengetahuan tentang pendidikan yang akan diselenggarakan dalam keluarga.
La producción y los productores agrícolas de Yahualica de González Gallo, Jalisco
Authors: Amador García Karla Anahí , García García José Odón
Published: March 2022

El objetivo del trabajo es analizar las características del sistema agrícola de Yahualica de González Gallo, Jalisco y clasificar a los productores según sus actividades y estrategias. A partir del análisis estadístico y de entrevistas a profundidad, con un cuestionario semiestructructurado de 117 preguntas, a una muestra de 136 actores clave, del padrón del CADER número 10 del 2019; se determinaron once tipos de productores, siendo: 1) agricultor tradicional de supervivencia, 2) con actividad complementaria, 3) comerciante, 4) ganadero, 5) ganadero comerciante, 6) chilero, 7) chilero emprendedor, 8) agricultor retornado, 9) retornado jubilado, 10) innovador y 11) el sustentable incipiente.
Comparison of Two Pre-Hospital Stroke Scales to Detect Large Vessel Occlusion Strokes in Australia: A Prospective Observational Study
Authors: Ostman Cecilia , Garcia-Esperon Carlos , Lillicrap Thomas , Alanati Khaled , Chew Beng Lim Alvin , Pedler Jennifer , Edwards Sarah , Parsons Mark , Levi Christopher , Spratt Neil
Published: March 2022

Aims

The Hunter-8 and the ACT-FAST are stroke scales used in Australia for the pre-hospital identification of large vessel occlusion (LVO) stroke but have not previously been compared. Moreover, their use in identifying distal arterial occlusions has not previously been assessed. We therefore aimed to describe the area under the receiver operating curve (AUC) of the Hunter-8 versus the ACT-FAST for the detection of classic LVO.

Methods

Both scales were performed on consecutive patients presenting with stroke-like symptoms within 24 hours of symptom onset presenting to the emergency department at a tertiary referral hospital between June 2018 and January 2019. The AUC of the Hunter-8 and the ACT-FAST was calculated for the detection of LVO using different definitions [classic LVO (proximal segment of the middle cerebral artery (MCA-M1), terminal internal carotid artery (T-ICA), or tandem occlusions) and extended LVO (classic LVO plus proximal MCA-M2 and basilar occlusions)].

Results

Of 126 suspected stroke patients, there were 24 classic LVO and 34 extended LVO. For detection of classic LVO, the Hunter-8 had an AUC of 0.79 and the ACT-FAST had an AUC of 0.77. For extended LVO, the AUC was 0.71 and 0.70 respectively.

Conclusion

Both scales represent a significant opportunity to identify patients with proven potential benefit from thrombectomy (classic LVO), however M2 and basilar occlusions may be more challenging to identify with these scales.
Identificación de ambientes de aprendizaje en la Universidad Tecnológica de Panamá motivado por la pandemia de la COVID-19
Authors: Chang Ignacio , Ríos Ana , Ríos Oscar
Published: March 2022

El objetivo de este trabajo es identificar metodologías y herramientas tecnológicas utilizadas en la Universidad Tecnológica de Panamá para crear espacios de aprendizaje que vayan acorde con la educación de nivel superior y con los retos actuales de la sociedad en la solución de sus problemas, por ende, es una revisión bibliográfica y del estado de la práctica en la institución. En este estudio, se aplicó una encuesta a profesores de todas las facultades en el Campus Central de la Universidad Tecnológica de Panamá para conocer las técnicas aplicadas en los cursos, las opiniones sobre estas y el conocimiento sobre técnicas aplicadas por sus colegas.
Análisis de aumento del nivel del mar en Isla Colón, Bocas del Toro
Authors: Grajales Saavedra Francisco , Ciniglio Stefano , Machado Víctor , Vallarino Ruby
Published: March 2022

El cambio climático genera afectaciones que amenazan la vida en el planeta como lo es el aumento del nivel del mar, situación que ha tomado un papel protagónico dentro de la preocupación mundial en los últimos años. Es por ello que organizaciones internacionales analizan estos impactos y generan planes para los tomadores de decisiones con miras a proteger la población. Panamá, al ser un país con costas en el océano Pacífico y mar Caribe, necesita la creación de mapas de inundación en zonas costeras, como la Isla Colón en la provincia de Bocas del Toro. Estos mapas son una herramienta para describir la magnitud y alcance del aumento del nivel del mar a una escala de tiempo en específico, encontrándose información valiosa en las proyecciones a los años 2050, 2080 y 2110. En este estudio se presentan mapas de inundación desarrollados con el programa Civil 3D, en el cual se utilizaron datos georreferenciados de Isla Colón suministrados por el Instituto Geográfico Nacional Tommy Guardia (IGNTG). Entre los resultados se detallan las zonas más afectadas y vulnerables ante el aumento del nivel del mar en la Isla en tres periodos. La percepción pública relacionada al aumento del nivel del mar se evaluó por medio de encuestas a 300 personas. De manera simultánea se buscó concientizar a la población acerca del cambio climático y la vulnerabilidad de zonas costeras ante el aumento del nivel del mar.
Aqua plantae: Sistema prototipo sostenible de desalinización de agua de mar, y aplicación a cultivos mediante riego subterráneo
Authors: Domínguez Viccelda , Atencio Alberto , Mojica Alexandra , Osorio Juan
Published: March 2022

En los últimos años, la problemática principal en el mundo es sin lugar a duda el inminente cambio climático acompañado de los fenómenos del Niño y la Niña que traen consigo, sequías extremas de un lado e inundaciones por el otro. A esto se le suma la contaminación del planeta y específicamente la de los ríos, lagos y otras fuentes de agua dulce que precisamente son las fuentes de agua que necesitan los seres vivos para su supervivencia. Esta investigación tiene como propósito adaptar un método de desalinización del agua de mar, a través de un procedimiento de destilación para la obtención de agua dulce, usando como fuente de energía, la solar. Por otra parte, se demuestra la eficiencia del producto de la desalinizadora, simulando microsistemas de crecimiento de plantas en función de la adición de agua de mar tratada, usando como caso de estudio muestras de suelo degradado del arco seco en la región de Los Santos. La metodología incluyó: i. Adaptar los métodos de Nicholas Grimshaw (Teatro del Agua) y Charlie Paton (Invernadero de Agua de Mar) sobre la utilización del agua de mar, ii. La aplicación de agua desalinizada, agua de mar, agua de lluvia y agua potable, a terrarios sembrados con maíz, frijol, lenteja y guandú en triplicado a escala de laboratorio. La tecnología es bastante sencilla y efectiva en la disminución de los niveles de salinidad en el agua de 34.39 g/L a 0.01 g/L; además, implica el uso de energía solar, la cual lo hace auto gestionable. Este estudio demuestra que sí es posible obtener agua de buena calidad por desalinización y de manera sostenible utilizando energía no convencional. Y que puede ser de grandes proporciones para el futuro del país beneficiando directamente a las regiones secas del país.
Una revisión de las estrategias de diseño de envolventes para minimización de consumo energético de edificaciones en clima tropical basado en enfoques biomiméticos
Authors: Chen Austin Miguel , Cowen Oscar , Delgado Nicole , González Chrissmar
Published: March 2022

La envolvente térmica de una edificación es la interface entre el ambiente exterior y los espacios habitables de su interior, y se considera uno de los aspectos más críticos para la gestión eficiente de la energía de calefacción, refrigeración e iluminación. En este artículo se presenta una revisión sobre distintos enfoques basados en estrategias biomiméticas, que pueden ser aplicados al diseño y construcción de envolventes térmicas autoajustables. Las estrategias de adaptación conductuales, morfológicas y fisiológicas, que tienen los organismos en la naturaleza, sirven de inspiración a la humanidad para lograr grandes avances como el diseño de estructuras inteligentes para edificaciones y la creación de materiales bioinspirados.
Multisystem Inflammatory Syndrome in Children and Kawasaki Disease: A Clinical Conundrum
Authors: Bansal Pankaj , Mangat Chetna
Published: March 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in general leads to a mild disease in children, but a rare yet serious complication of multisystem inflammatory syndrome in children (MIS-C) has been reported.1 MIS-C cases are on the rise during the COVID-19 pandemic, and clinical features overlap between MIS-C and an already well-known clinical entity in children: Kawasaki disease (KD). Both KD and MIS-C are diagnoses of exclusion, and can present with acute fever and increased inflammatory markers without any other potential etiology.2 Despite the overlap in clinical presentation, there are some differentiating features that could help establish an accurate diagnosis and appropriate management, which are highlighted in this article.
Crowdsourcing the Historical Record: Creating Linked Open Data for Chinese History at Scale
Authors: Sturgeon Donald
Published: March 2022

An important part of the historical record of premodern China is recorded in historical works such as the standard dynastic histories. These works are a key source of knowledge about many aspects of premodern Chinese civilization, including persons, events, bureaucratic structures, literature, geography and astronomical observations. While many such sources have been digitized, typically these digitized texts encode only literal textual content and do not attempt to model the semantic content of the text. Similarly, while some of the historical data contained in some of these sources has been entered into specialist scholarly databases, an even greater proportion of the information does not yet exist in any machine-readable form.

Producing such a machine-readable dataset of these materials requires the effort of many individuals working together due to the large scale of the task. This article introduces a crowdsourced approach in which annotation and knowledge base construction are carried out in parallel, with a knowledge base continually expanded through multi-user contributions to textual annotation immediately and automatically feeding back to provide improved assistance with subsequent annotation. The resulting knowledge base is dynamically exposed through Linked Open Data interfaces, creating a continually expanding machine-readable dataset covering around 3,000 years of recorded history.
Linked Open Data and Medieval Studies: Some Lessons from the Mapping Manuscript Migrations Project
Authors: Burrows Toby
Published: March 2022

Building on the work of the Mapping Manuscript Migrations (MMM) Project between 2017 and 2020, this article aims to identify the potential benefits and likely challenges of using Linked Open Data (LOD) more widely across the research field of medieval studies. As well as aggregating and linking disparate datasets relating to the history of more than 220,000 medieval manuscripts, the MMM Project reconciled and matched vocabularies for places, persons, organizations, works and manuscripts. It built and tested various forms of access to the aggregated data, including a web portal and a SPARQL endpoint. It also demonstrated suitable ways of publishing its outputs, not only the aggregated data but also the data model and ontologies used. Drawing on the lessons learned in the MMM Project, the article offers suggestions for building an LOD environment for Western European medieval studies more broadly, covering the aggregation of heterogeneous data, the reconciliation of disparate vocabularies, and ways of enabling more effective discovery, exploration and analysis across the aggregated data.
La innecesaria regulación laboral de los trabajadores que prestan sus servicios en plataformas de digitales. Una cuestión inacaba
Authors: Macías García María del Carmen
Published: March 2022

A raíz de la última sentencia del Tribunal Supremo que declara laboral por cuenta ajena la relación de los trabajadores que prestan sus servicios para plataformas digitales parece que se ha dado respuesta a la -inexistente- duda sobre la laboralidad que rodean estos repartidores. Tanto es así que el Gobierno ha aprobado, ex profeso, el RD-Ley 9/2021 que introduce la presunción de laboralidad de estos trabajadores. Sin embargo, y teniendo en cuenta los antecedentes jurisprudenciales de nuestro Alto Tribunal en cuestiones similares, nos preguntamos si era necesario dilatar tanto esta cuestión en el tiempo o por el contrario se podría desde un inicio, y sin vacilaciones, haber zanjado la cuestión optando, como es de justicia, y así se viene reiterando en concretar que dicha prestación de servicios se ajusta a la relación laboral por cuenta ajena definida por la norma. Intentaremos, por tanto, dar respuesta a dicha cuestión mediante el análisis de la legislación, la jurisprudencia y la forma de trabajar que presentan dichas empresas.