bluuebunny/crossref_metadata_2025 huggingface dataset:
Archaeal tRNA-Splicing Endonuclease as an Effector for RNA Recombination and Novel Trans-Splicing Pathways in Eukaryotes
Authors: Tocchini-Valentini Giuseppe D. , Tocchini-Valentini Glauco P.
Published: December 2021

We have characterized a homodimeric tRNA endonuclease from the euryarchaeota Ferroplasma acidarmanus (FERAC), a facultative anaerobe which can grow at temperatures ranging from 35 to 42 °C. This enzyme, contrary to the eukaryal tRNA endonucleases and the homotetrameric Methanocaldococcus jannaschii (METJA) homologs, is able to cleave minimal BHB (bulge–helix–bulge) substrates at 30 °C. The expression of this enzyme in Schizosaccharomyces pombe (SCHPO) enables the use of its properties as effectors by inserting BHB motif introns into hairpin loops normally seen in mRNA transcripts. In addition, the FERAC endonuclease can create proteins with new functionalities through the recombination of protein domains.
Functional Profile of Older Adults Hospitalized in Convalescence Units of the National Network of Integrated Continuous Care of Portugal: A Longitudinal Study
Authors: Ramos Ana , Fonseca César , Pinho Lara , Lopes Manuel , Oliveira Henrique , Henriques Adriana
Published: December 2021

Aim: To evaluate the evolution of the functional profile of older adults admitted to a health unit in Portugal; to relate the functional profile of these individuals with age, sex, education level and emotional state; and to evaluate the probability of the degree of dependence as a function of age and sex. Methods: longitudinal, retrospective study with a sample of 59,013 older adults admitted to convalescence units of the National Network of Integrated Continuous Care of Portugal. Results: In the first 75 days of hospitalization, activities of daily living, mobility and cognitive state improved, but there was a decline after 75 days of hospitalization. The ability to perform instrumental activities of daily living improved in the first 15 days of hospitalization, stabilized until 45 days and then began to worsen. Women had a higher probability of having a severe/complete dependence three years earlier than men (88 years to 91 years). A higher education level and stable emotional state were protective factors against functional decline. Conclusions: The functional profile of older adults improved during the length of stay recommended for hospitalization in convalescence units (30 days). It is critical for health systems to adopt strategies to prevent declines in the emotional state of frail individuals.
Prognostic Relevance of Thyroid-Hormone-Associated Proteins in Adenoid Cystic Carcinoma of the Head and Neck
Authors: Schnoell Julia , Kotowski Ulana , Jank Bernhard J. , Stoiber Stefan , Gurnhofer Elisabeth , Schlederer Michaela , Heiduschka Gregor , Kenner Lukas , Kadletz-Wanke Lorenz
Published: December 2021

The proteins sodium iodide symporter (NIS), μ-crystallin (CRYM), and thyroid hormone receptor beta (THRB) have been associated with prognosis in various cancer entities. While NIS and THRB may serve as possible therapeutic targets, the role of CRYM in cancer is still unclear. Protein levels of 44 patients with adenoid cystic carcinoma of the head and neck were analyzed using immunohistochemistry and correlated with clinicopathological data and outcome. NIS was positive in 72%, CRYM was positive in 55%, and THRB was positive in 39% of the patients. CRYM-positive adenoid cystic carcinomas were associated with a better cause-specific survival. Thus, our data indicate that CRYM might be a suitable positive prognostic marker in adenoid cystic carcinoma of the head and neck. Furthermore, expression of NIS was present in most patients and therefore evaluation of the use of radioiodine treatment is recommended.
The Need for Precision Therapies as Determined by Genetic Signature for Cystic Fibrosis
Authors: Zahir Farah R.
Published: December 2021

Cystic fibrosis (CF) is a devastating genetic infant-onset disease [...]
Weight Loss at First Month and Development of Tolerance as Possible Predictors of 30 mg Phentermine Efficacy at 6 Months
Authors: Rocha-González Héctor Isaac , De la Cruz-Álvarez Lidia Elizabeth , Kammar-García Ashuin , Canizales-Quinteros Samuel , Huerta-Cruz Juan Carlos , Barranco-Garduño Lina Marcela , Reyes-García Juan Gerardo
Published: December 2021

The efficacy of anti-obesity drugs usually does not consider the high degree of interindividual variability in responses to the drug which could affect the decision to withdraw the drug early due to ineffectiveness or to continue therapy according to specific expectations of success. The aim of this study was to analyze body weight loss in kilograms during the first month (1 mo-BWLkg) of treatment with 30 mg phentermine and development of tolerance to phentermine, on its 6-month efficacy. One hundred sixty-six subjects with obesity were individually or jointly analyzed in the study. Subjects with 1 mo-BWLkg of <1 kg, 1–3 kg, 3–5 kg, and ≥5 kg reached 6-month mean percentage body weight reductions (BWR%) of approximately 3%, 5%, 10%, and 15%, respectively. Development of late tolerance (4–6 months) to phentermine had a lower impact than early tolerance (2–3 months). Subjects with 1 mo-BWLkg < 3 kg who developed early tolerance did not achieve relevant BWR% (≥5%) at month 6, while the rest of the subgroups achieved increasing and progressive BWR%, according to their 1 mo-BWLkg range and time of onset of tolerance. The 1 mo-BWLkg and development of tolerance to phentermine could be useful to predict the expected 6-month efficacy trends in obese patients treated with 30 mg phentermine.
Nursing Students’ Practicums during the COVID-19 Crisis and the Effect on Infection-Prevention Behavior in Students: A Mixed-Method Approach
Authors: Nakagawa Hiromi , Sasai Hiroyuki
Published: December 2021

Background and Objectives: The coronavirus disease pandemic is ongoing. Infection-prevention measures in nursing education (practicum) are essential. However, there are few studies on infection-prevention behaviors among nursing students participating in practicums. We aimed to clarify the effect of practicums during the coronavirus disease crisis on infection-prevention behavior in Japanese nursing students. Materials and Methods: We conducted semi-structured interviews with 13 third-year nursing students in Osaka City within one week of their clinical placement training. From the results of the interview analysis, we compiled a questionnaire and surveyed 90 third-year students. We conducted qualitative and quantitative analyses. We used descriptive statistics for the quantitative analysis and the chi-squared test for binary variables. Results: From the qualitative analysis, we identified five categories regarding the awareness of infection-prevention measures: , , , , and . In the quantitative analysis, the practicum students who attended at least three pre-practicum orientations continued wearing masks during lunch breaks and avoided the three Cs. Conclusions: Students could recall the knowledge and experiences gained from pre-practicum orientations/practicums. This experience created a new awareness of infection-prevention and change of infection-prevention behavior. Infection-prevention education using practicums is important for infection-prevention behavior during this pandemic. However, there should be a much larger-scale study to support these findings in the future.
Numerical Analysis and Parameter Optimization of Wear Characteristics of Titanium Alloy Cross Wedge Rolling Die
Authors: Peng Zhanshuo , Ji Hongchao , Huang Xiaomin , Wang Baoyu , Xiao Wenchao , Wang Shufu
Published: December 2021

Cross wedge rolling has the advantages of high production efficiency, good product quality, high material utilization, environmental protection, and low cost. It is one of the best processing methods for producing shaft blanks. In this paper, a cross wedge rolling die of TC4 titanium alloy is studied. Based on the Archard wear model, a modified model suitable for cross wedge rolling die wear analysis is derived through finite element simulation. Then, the modified Archard wear model is imported into Deform-3D software for finite element analysis. Orthogonal experimental design is used to combine and analyze different process parameters. Finally, the beetle antennae search (BAS)-genetic algorithm (GA)-back propagation neural network (BPNN) algorithm is used to predict the degree of die wear and to optimize the simulation parameters, which can acquire the process parameters that have the least impact on die wear. The results show that the wear distributions of cross wedge rolling tools is uneven. In general, the most serious areas are basically concentrated in the wedge-shaped inclined plane and rectangular edge lines. The reason is that the tangential force and radial force received by the die are relatively large, which leads to increased wear. Moreover, the temperature change is most severe on the wedge-shaped ridge line. When in contact with the workpiece, the temperature rises sharply, which makes the local temperature rise, the mold hardness decrease, and the wear accelerate. Through response surface method (RSM) analysis, it is concluded that the deformation temperature is the main factor affecting wear depth, followed by the forming angle, and that there is an interaction between the two factors. Finally, the feasibility of the BAS-GA-BP algorithm for optimizing the wear behavior of dies is verified, which provides a new process parameter optimization method for the problem of die wear in the cross wedge rolling process.
Dislocation Loop Generation Differences between Thin Films and Bulk in EFDA Pure Iron under Self-Ion Irradiation at 20 MeV
Authors: Roldán Marcelo , Sánchez Fernando José , Fernández Pilar , Ortiz Christophe J. , Gómez-Herrero Adrián , Rey David Jiménez
Published: December 2021

In the present investigation, high-energy self-ion irradiation experiments (20 MeV Fe+4) were performed on two types of pure Fe samples to evaluate the formation of dislocation loops as a function of material volume. The choice of model material, namely EFDA pure Fe, was made to emulate experiments simulated with computational models that study defect evolution. The experimental conditions were an ion fluence of 4.25 and 8.5 × 1015 ions/cm2 and an irradiation temperature of 350 and 450 °C, respectively. First, the ions pass through the samples, which are thin films of less than 100 nm. With this procedure, the formation of the accumulated damage zone, which is the peak where the ions stop, and the injection of interstitials are prevented. As a result, the effect of two free surfaces on defect formation can be studied. In the second type of experiments, the same irradiations were performed on bulk samples to compare the creation of defects in the first 100 nm depth with the microstructure found in the whole thickness of the thin films. Apparent differences were found between the thin foil irradiation and the first 100 nm in bulk specimens in terms of dislocation loops, even with a similar primary knock-on atom (PKA) spectrum. In thin films, the most loops identified in all four experimental conditions were b ±a0<100>{200} type with sizes of hundreds of nm depending on the experimental conditions, similarly to bulk samples where practically no defects were detected. These important results would help validate computational simulations about the evolution of defects in alpha iron thin films irradiated with energetic ions at large doses, which would predict the dislocation nucleation and growth.
Magnetic Pulse Hybrid Joining of Polymer Composites to Metals
Authors: Khalil Chady , Marya Surendar , Racineux Guillaume
Published: December 2021

To lighten their vehicles, car manufacturers are inclined to substitute steel structures with aluminum alloys or composites parts. They are then faced with the constraints inherent to dissimilar (galvanized steel/aluminum) or hybrid (metal/composite) assemblies. Recent developments in magnetic pulse welding seems to offer a viable route. Very fast, this process can be robotized and generates a very localized heating system which limits the formation of intermetallic and damage the composite. Low energy consumption, without filler metal or smoke it is recognized as an environmentally friendly process. In this paper, electromagnetic pulse welding is exploited to assemble polymer composite to metals. Two techniques, a metallic insert in polymer composite or an external patch, have been tested with possible design considerations.
Numerical Analysis of Particle Trajectories in a Gas–Powder Jet during the Laser-Based Directed Energy Deposition Process
Authors: Stankevich Stanislav , Larionov Nikolay , Valdaytseva Ekaterina
Published: December 2021

Based on numerical solutions of the equation of motion of a particle in a gas jet modeled by the Reynolds-averaged Navier–Stokes equations, the features of transporting powder particles to the working zone of laser-based directed energy deposition are investigated. The propagation of a gas jet in a confined space in the presence of obstacles in the form of a substrate and a wall of a part is considered. A solution determining the gas-dynamic parameters of the jet is obtained, and the results of calculating its velocity field are presented. The influence of gas-dynamic parameters on the trajectories of the powder particles is analyzed. It is shown that these parameters determine the amount of model material involved in the formation of the geometry of the part.
Effect of Ce Content on Microstructure-Toughness Relationship in the Simulated Coarse-Grained Heat-Affected Zone of High-Strength Low-Alloy Steels
Authors: Cao Yuxin , Wan Xiangliang , Zhou Feng , Wang Yong , Liu Xinbin , Wu Kaiming , Li Guangqiang
Published: December 2021

The study aimed to identify a moderate degree of Ce addition to improve the toughness in the simulated coarse-grained heat-affected zone (CGHAZ) of high-strength low-alloy steels, based on the effect of the Ce content on particle characteristics, microstructure and impact toughness. Three steels with 0.012 wt.%, 0.050 wt.% and 0.086 wt.% Ce content were subjected to 100 kJ/cm heat input in their thermal welding cycles. The particles and microstructures in the simulated CGHAZ of each steel were characterized and the impact-absorbance energy levels were measured at −20 °C. The results indicated that Ce2O2S inclusion compounds were gradually modified to CexSy-CeP and CeP with the increasing of the Ce content. A higher fraction of acicular ferrite was formed in the 0.012 wt.%-Ce-treated steel due to the lower mismatch between Ce2O2S and α-Fe. Furthermore, a lower fraction of M-A constituent was obtained in the 0.012 wt.%-Ce-treated steel. As a result, superior toughness and a typical amount of ductile fracture were detected in the simulated CGHAZ of the 0.012 wt.%-Ce-treated steel. Compared with the 0.012 wt.%-Ce-treated steel, a smaller prior austenite grain was observed in the 0.086 wt.%-Ce-treated steel because of the segregation of CeP at the grain boundary. However, the larger size and density of CeP led to poor toughness in the CGHAZ of the 0.086 wt.%-Ce-treated steel.
Research Progress of Nano Copper Lubricant Additives on Engineering Tribology
Authors: Guo Junde , Zhao Yingxiang , Sun Biao , Wang Puchao , Wang Zhijie , Dong Hao
Published: December 2021

Nanoparticles have as characteristics super sliding, extreme pressure, self-healing, etc., which can improve the friction reduction and anti-wear performance of sliding components, when used as lubricating oil additives. Nano-copper particles have a good synergistic effect with other antifriction agents, anti-wear agents, antioxidants and grease additives because of their low shear strength and grain boundary slip effect, showing a better anti-friction and anti-wear effect. However, nanoparticles are prone to conglomerate, and this causes a bottleneck in the application of dispersant for nano-copper in a lubricating oil system. The regulation of nanosized effect and surface properties has great engineering significance in compensating for the precision in manufacturing accuracy. This paper comprehensively reviews the tribological research progress of nano-copper as a lubricant additive, which provides a reference to the application of nano-copper particles as lubricating oil additives on engineering tribology.
Sequential Oxidation Strategy for the Fabrication of Liquid Metal Electrothermal Thin Film with Desired Printing and Functional Property
Authors: Fu Jun-Heng , Zhang Xu-Dong , Qin Peng , Liu Jing
Published: December 2021

Room temperature liquid metal (LM) showcases a great promise in the fields of flexible functional thin film due to its favorable characteristics of flexibility, inherent conductivity, and printability. Current fabrication strategies of liquid metal film are substrate structure specific and sustain from unanticipated smearing effects. Herein, this paper reported a facile fabrication of liquid metal composite film via sequentially regulating oxidation to change the adhesion characteristics, targeting the ability of electrical connection and electrothermal conversion. The composite film was then made of the electrically resistive layer (oxidizing liquid metal) and the insulating Polyimide film (PI film) substrate, which has the advantages of electrical insulation and ultra-wide temperature working range, and its thickness is only 50 μm. The electrical resistance of composite film can maintain constant for 6 h and could work normally. Additionally, the heating film exhibited excellent thermal switching characteristics that can reach temperature equilibrium within 100 s, and recovery to ambient temperature within 50 s. The maximum working temperature of the as-prepared film is 115 °C, which is consistent with the result of the theoretical calculation, demonstrating a good electrothermal conversion capability. Finally, the heating application under extreme low temperature (−196 °C) was achieved. This conceptual study showed the promising value of the prototype strategy to the specific application areas such as the field of smart homes, flexible electronics, wearable thermal management, and high-performance heating systems.
Low-Frequency, Open, Sound-Insulation Barrier by Two Oppositely Oriented Helmholtz Resonators
Authors: Guan Yi-Jun , Ge Yong , Sun Hong-Xiang , Yuan Shou-Qi , Liu Xiao-Jun
Published: December 2021

In this work, a low-frequency, open, sound-insulation barrier, composed of a single layer of periodic subwavelength units (with a thickness of λ/28), is demonstrated both numerically and experimentally. Each unit was constructed using two identical, oppositely oriented Helmholtz resonators, which were composed of a central square cavity surrounded by a coiled channel. In the design of the open barrier, the distance between two adjacent units was twice the width of the unit, showing high-performance ventilation, and low-frequency sound insulation. A minimum transmittance of 0.06 could be observed around 121.5 Hz, which arose from both sound reflections and absorptions, created by the coupling of symmetric and asymmetric eigenmodes of the unit, and the absorbed sound energy propagating into the central cavity was greatly reduced by the viscous loss in the channel. Additionally, by introducing a multilayer open barrier, a broadband sound insulation was obtained, and the fractional bandwidth could reach approximately 0.19 with four layers. Finally, the application of the multilayer open barrier in designing a ventilated room was further discussed, and the results presented an omnidirectional, broadband, sound-insulation effect. The proposed open, sound-insulation barrier with the advantages of ultrathin thickness; omnidirectional, low-frequency sound insulation; broad bandwidth; and high-performance ventilation has great potential in architectural acoustics and noise control.
Fabricating Silicon Resonators for Analysing Biological Samples
Authors: Kumemura Momoko , Pekin Deniz , Menon Vivek , Van Seuningen Isabelle , Collard Dominique , Tarhan Mehmet
Published: December 2021

The adaptability of microscale devices allows microtechnologies to be used for a wide range of applications. Biology and medicine are among those fields that, in recent decades, have applied microtechnologies to achieve new and improved functionality. However, despite their ability to achieve assay sensitivities that rival or exceed conventional standards, silicon-based microelectromechanical systems remain underutilised for biological and biomedical applications. Although microelectromechanical resonators and actuators do not always exhibit optimal performance in liquid due to electrical double layer formation and high damping, these issues have been solved with some innovative fabrication processes or alternative experimental approaches. This paper focuses on several examples of silicon-based resonating devices with a brief look at their fundamental sensing elements and key fabrication steps, as well as current and potential biological/biomedical applications.
Open-Source FPGA Coprocessor for the Doppler Emulation of Moving Fluids
Authors: Ricci Stefano
Published: December 2021

Embedded systems are nowadays employed in a wide range of application, and their capability to implement calculation-intensive algorithms is growing quickly and constantly. This result is obtained by the exploitation of powerful embedded processors that are often connected to coprocessors optimized for a particular application. This work presents an open-source coprocessor dedicated to the real-time generation of a synthetic signal that mimics the echoes produced by a moving fluid when investigated by ultrasounds. The coprocessor is implemented in a Field Programmable Gate Array (FPGA) device and integrated in an embedded system. The system can replace the complex and inaccurate flow-rigs employed in laboratorial tests of Doppler ultrasound systems and methods. This paper details the coprocessor and its standard interfaces, and shows how it can be integrated in the wider architecture of an embedded system. Experiments showed its capability to emulate a fluid flowing in a pipe when investigated by an echographic Doppler system.
Flexible Microwave Biosensor for Skin Abnormality Detection Based on Spoof Surface Plasmon Polaritons
Authors: Bai Junkai , Guo Hongfu , Li Hua , Zhou Chen , Tang Hanchao
Published: December 2021

Point-of-care testing plays an important role in the detection of skin abnormalities. The detection of skin abnormalities requires sufficient depth and no harm. A flexible microwave biosensor based on spoof surface plasmon polaritons was designed to meet the requirements of skin abnormalities. The designed biosensor, which works at 11.3 GHz, is small and can be flexibly attached to the skin surface of any part of the human body for measurement. The health status of the skin can be evaluated by the resonant frequency and the magnitude of the reflection coefficient of the sensor. The sensor was tested on pork skin. The experiment results showed that the sensor can detect skin abnormalities such as skin burn, skin tumor, and others. Compared with other sensors, the sensor has sufficient penetration depth because of the strong penetration of microwave electromagnetic waves. It is the first flexible microwave biosensor used for skin, which involves point-of-care testing, and continuous monitoring of skin.
Atmosphere Effect in Post-Annealing Treatments for Amorphous InGaZnO Thin-Film Transistors with SiOx Passivation Layers
Authors: Zhang Wen , Fan Zenghui , Shen Ao , Dong Chengyuan
Published: December 2021

We investigated the electrical performance and positive bias stress (PBS) stability of the amorphous InGaZnO thin-film transistors (a-IGZO TFTs) with SiOx passivation layers after the post-annealing treatments in different atmospheres (air, N2, O2 and vacuum). Both the chamber atmospheres and the device passivation layers proved important for the post-annealing effects on a-IGZO TFTs. For the heat treatments in O2 or air, the larger threshold voltage (VTH) and off current (IOFF), smaller field-effect mobility (μFE), and slightly better PBS stability of a-IGZO TFTs were obtained. The X-ray photoemission spectroscopy (XPS) and secondary ion mass spectroscopy (SIMS) measurement results indicated that the oxygen atoms from the ambience led to less oxygen vacancies (VO) and more oxygen-related defects in a-IGZO after the heat treatments in O2 or air. For the annealing processes in vacuum or N2, the electrical performance of the a-IGZO TFTs showed nearly no change, but their PBS stability evidently improved. After 4500 seconds’ stressing at 40 V, the VTH shift decreased to nearly 1 V. In this situation, the SiOx passivation layers were assumed to effectively prevent the oxygen diffusion, keep the VO concentration unchanged and refuse the oxygen-related defects into the a-IGZO films.
New Geological Evidence of the 1755 Lisbon Tsunami from the Rock of Gibraltar (Southern Iberian Peninsula)
Authors: Ruiz Francisco , Pozo Manuel , González-Regalado María Luz , Rodríguez Vidal Joaquín Rodríguez , Cáceres Luis Miguel , Abad Manuel , Izquierdo Tatiana , Prudencio María Isabel , Dias María Isabel , Marques Rosa , Muñoz-Pichardo Juan M. , Tosquella Josep , Gómez Paula , Toscano Antonio , Romero Verónica , Arroyo Marta
Published: December 2021

This paper presents the easternmost mineralogical and geochemical evidence of the 1755 Lisbon tsunami found in the Western Mediterranean. This multidisciplinary analysis of a sediment core obtained in Gibraltar (southern Iberian Peninsula) has allowed us to differentiate a tsunamiite from an old lagoon (The Inundation). This tsunamigenic layer has increased levels of calcite and aragonite and higher concentrations of Ba and ferromagnesian elements in comparison with the underlying lagoonal sediments of this core. This layer is also differentiated by its paleontological record, with the introduction of marine species within this lagoon. The uppermost part of the core includes a transition from swampy/marsh paleoenvironments to terrestrial scenarios, with a final anthropogenic filling occurring during the last century.
Detrital Zircon Provenance of the Cenozoic Sequence, Kotli, Northwestern Himalaya, Pakistan; Implications for India–Asia Collision
Authors: Awais Muhammad , Qasim Muhammad , Tanoli Javed Iqbal , Ding Lin , Sattar Maryam , Baig Mirza Shahid , Pervaiz Shahab
Published: December 2021

This study reported the detrital zircon U-Pb geochronology of the Cenozoic sequence exposed in Kotli, northwestern Himalaya, Pakistan, which forms part of the Kashmir foreland basin. The U-Pb detrital age patterns of the Paleocene Patala Formation show a major age cluster between ~130–290 Ma, ~500–1000 Ma and ~1000–1500 Ma, which mainly resembles the lesser and higher Himalayan sequence. However, the younger age pattern (~130–290 Ma) can be matched to the ages of the ophiolites exposed along the Indus–Tsangpo suture zone. In addition, two younger grains with 57 Ma and 55 Ma ages may indicate a contribution from the Kohistan-Ladakh arc. The detrital zircons in the upper Tertiary sequence show the increased input of younger detrital ages <100 Ma, with more pronounced peaks at ~36–58 Ma, ~72–94 Ma and ~102–166 Ma, indicating the strong resemblance to the Asian sources including the Kohistan–Ladakh arc, Karakoram block and Gangdese batholith. This provenance shift, recorded in the upper portion of Patala Formation and becoming more visible in the upper Tertiary clastic sequence (Kuldana and Murree formations), is related to the collision of the Indian and Asian plates in the northwestern Himalayas. Considering the age of the Patala Formation, we suggest that the Indian and Asian plates collided during 57–55 Ma in the northwestern Himalayas, Pakistan.
Supergene Copper and the Ancient Mining Landscapes of the Atacama Desert: Refining the Protocol for the Study of Archaeological Copper Minerals through the Case Study of Pukara de Turi
Authors: Sapiains Pía , Figueroa Valentina , Hayashida Frances , Salazar Diego , Menzies Andrew , González Cristián , Loyola Rodrigo , Murphy Beau , González Juan , Parcero-Oubiña César , Troncoso Andrés
Published: December 2021

Northern Chile is home to the world’s largest copper ore deposits, which have been exploited for thousands of years by different groups, at varying scales and for different purposes. In this context, it is important to develop new protocols to characterise the mineralogical variability of archaeological copper ores. A comprehensive and representative methodology in the analysis of minerals, the application of non-destructive analytical techniques, and the combination of insights from geological, archaeological and local knowledge are key to developing a copper mineral repository of the Atacama Desert area. Geochemical analyses were applied to the study of 568 samples from the archaeological site Pukara de Turi, with different techniques such as micro-XRF, XRD, QEMSCAN, Raman spectroscopy and technological studies. This exhaustive analysis allowed for the recognition of two mineralogical associations: atacamite/brochantite (99%) and azurite/chrysocolla (1%). The study of various minerals allows data to be interpreted more reliably and to trace the likely geological sources of these minerals. The azurite/chrysocolla samples appear to belong to the same mineral association found in the Cerro Verde district, which is probably the source of these samples. The atacamite/brochantite samples appear to come from more than one geological source, including, but not limited to, Chuquicamata-Radomiro Tomic and El Abra-Conchi.
New Evidence of the Brizziite, Sodium Antimonate from the Central Paratethys Sea Strata in Poland
Authors: Gołębiowska Bożena , Pilarz Monika , Bukowski Krzysztof
Published: December 2021

Brizziite, a rare sodium antimonate (NaSb5+O3), and fluorcalcioroméite ((Ca,Na)2Sb5+2O6F), have been identified in two boreholes (Pasternik and Włosienica) which are situated 50 km apart. Both wells are located west of Krakow, Poland, and were drilled in the Miocene strata of the Paratethys Sea (a remnant of the Tethys Ocean). The Sb minerals are scattered in a solidified light blue silica gel within marls and layered clays. They occur most often as anhedral grains up to 20 μm in size. The presence of these phases was confirmed by Raman spectroscopy (RS) and X-ray diffraction (XRD). The brizziite from this study represents a secondary mineral after the alteration of roméite within a supergene zone, or crystallization from Sb-rich solutions derived by the leaching of the weathered primary roméite. Hence, the calcium and fluorine admixtures in their composition, determined by EPMA, indicate intergrowths of brizziite and roméite on the micro- to crypto-scale. The presence of the antimony in the study area is related to rejuvenated Old-Paleozoic polymetallic ore-mineralization occurring in the basement of the Krakow-Silesia Monocline. The phenomenon of the repeatability of brizziite in Pasternik and Włosienica, distant by several tens of kilometers, can be explained by the following three steps: (i) the penetration of the chloride ions from the drying up seawaters of the Paratethys Sea into the Miocene groundwater system, (ii) the mobilization of Sb5+ in the form of chloride complexes, and, finally, (iii) the transportation of the Sb-bearing solutions within the marly and clay sediments.
Mining-Induced Stress Control by Advanced Hydraulic Fracking under a Thick Hard Roof for Top Coal Caving Method: A Case Study in the Shendong Mining Area, China
Authors: Zheng Kaige , Liu Yu , Zhang Tong , Zhu Jingzhong
Published: December 2021

Fully mechanized top-coal caving mining with high mining height, hard roofs and strong mining pressure are popular in the Shendong mining area, China. The occurrence of dynamic disasters, such as rock burst, coal and gas outburst, mine earthquakes and goaf hurricanes during the coal exploitation process under hard roof conditions, pose a threat to the safe production of mines. In this study, the characteristics of overburden fracture in fully mechanized top-coal caving with a hard roof and high mining height are studied, and the technology of advanced weakening by hard roof staged fracturing was proposed. The results show that the hard roof strata collapse in the form of large “cantilever beams”, and it is easy to release huge impact kinetic energy, forming impact disasters. After the implementation of advanced hydraulic fracturing, the periodic weighting length decreases by 32.16%, and the length of overhang is reasonably and effectively controlled. Ellipsoidal fracture networks in the mining direction of the vertical working face, horizontal fracture networks perpendicular to the direction of the working face, and near-linear fracture planes dominated by vertical fractures were observed, with the accumulated energy greatly reduced. The effectiveness of innovation technology is validated, and stress transfer, dissipation and dynamic roof disasters were effectively controlled.
Effect of Lattice Substitution on Adsorption of Hexavalent Chromium by Montmorillonite, Nontronite, and Beidellite
Authors: Luo Weige , Zeng Zheng , Bian Liang
Published: December 2021

This work aims to evaluate the effect of lattice substitution on adsorption of hexavalent chromium by three kinds of typical clay minerals, and its common isomorph via experiments and calculations were performed based on density functional theory. The experiments (25 °C, 4 h, pH = 4 and without stirring) confirmed an order of adsorption capacity as follows: Montmorillonite (12 mg/g) > Nontronite (9 mg/g) > Beidellite (8 mg/g). Accordingly, the Mulliken populations, density of states, and band structures of the mineral models with the structural Al, Mg, Fe(II), Fe(III), and Al (in tetrahedrons) on behalf of five species of isomorph were calculated. The calculation results explain the differences between hexavalent chromium adsorption capacity of five kinds of isomorph by means of atom, key populations, overlapping valence electron orbitals, and the variation of energy band. However, no overlapping orbitals were observed in the adsorption system with structural Mg. It is implied that the structural Mg has little influence of hexavalent chromium adsorption. In conclusion, our study contributes to achieving a better understanding of modified clay minerals materials applications.
Investigation on the Failure Mechanism of Weak Floors in Deep and High-Stress Roadway and the Corresponding Control Technology
Authors: Zhang Dong , Bai Jianbiao , Yan Shuai , Wang Rui , Meng Ningkang , Wang Gongyuan
Published: December 2021

Large deformation of roadway and floor burst are the two major geotechnical hazards encountered with high mining stress in deep mines. In this paper, the stress and energy conditions generated by the impact damage on the rock surrounding a roadway are analyzed, and UDEC software was used to study the deformation characteristics of the roadway, as well as its failure mechanism under the influence of superimposed dynamic and static loads. The results indicate that the soft floor of a deep-buried roadway has a high damage degree and an obvious stress release effect, high static load leads to slow floor heave, and strong dynamic load disturbance is the principal trigger leading to floor burst. In addition, the anisotropy caused by the bedding surface weakens the cooperative characteristics of the support system, resulting in serious instability of the whole rock surrounding the roadway. Full-section anchor cables and inverted arches were adopted to maintain the stability of the rock surrounding the roadway. The monitoring results obtained from field tests show that the adoption of the combined support system effectively avoids floor burst caused by the superposition of dynamic and static loads; the maximum floor heave is 67.9 mm, which is 95% lower than the original value, ensuring safety in coal mining operations.
Enhanced Photocurrent of the Ag Interfaced Topological Insulator Bi2Se3 under UV- and Visible-Light Radiations
Authors: Wang Chih-Chiang , Lin Pao-Tai , Shieu Fuh-Sheng , Shih Han-Chang
Published: December 2021

Bi2Se3 is a topological quantum material that is used in photodetectors, owing to its narrow bandgap, conductive surface, and insulating bulk. In this work, Ag@Bi2Se3 nanoplatelets were synthesized on Al2O3(100) substrates in a two-step process of thermal evaporation and magnetron sputtering. X-ray diffractometer (XRD), high-resolution transmission electron microscopy (HRTEM), Raman spectroscopy, and x-ray photoelectron spectroscopy (XPS) revealed that all samples had the typical rhombohedral Bi2Se3. Field-emission scanning electron microscopy (FESEM)-energy dispersive x-ray spectroscopy (EDS), XPS, and HRTEM confirmed the presence of the precipitated Ag. The optical absorptance of Bi2Se3 nanoplatelets in UV-visible range decreased with the Ag contents. Results of photocurrent measurements under zero-bias conditions revealed that the deposited Ag affected photosensitivity. A total of 7.1 at.% Ag was associated with approximately 4.25 and 4.57 times higher photocurrents under UV and visible light, respectively, than 0 at.% Ag. The photocurrent in Bi2Se3 at 7.1 at.% Ag under visible light was 1.72-folds of that under UV light. This enhanced photocurrent is attributable to the narrow bandgap (~0.35 eV) of Bi2Se3 nanoplatelets, the Schottky field at the interface between Ag and Bi2Se3, the surface plasmon resonance that is caused by Ag, and the highly conductive surface that is formed from Ag and Bi2Se3. This work suggests that the appropriate Ag deposition enhances the photocurrent in, and increases the photosensitivity of, Bi2Se3 nanoplatelets under UV and visible light.
Subtractive Low-Temperature Preparation Route for Porous SiO2 Used for the Catalyst-Assisted Growth of ZnO Field Emitters
Authors: Haugg Stefanie , Hedrich Carina , Blick Robert H. , Zierold Robert
Published: December 2021

The possibility to gradually increase the porosity of thin films facilitates a variety of applications, such as anti-reflective coatings, diffusion membranes, and the herein investigated tailored nanostructuring of a substrate for subsequent self-assembly processes. A low-temperature (<160 °C) preparation route for porous silicon oxide (porSiO2) thin films with porosities of about 60% and effective refractive indices down to 1.20 is tailored for bulk as well as free-standing membranes. Subsequently, both substrate types are successfully employed for the catalyst-assisted growth of nanowire-like zinc oxide (ZnO) field emitters by metal organic chemical vapor deposition. ZnO nanowires can be grown with a large aspect ratio and exhibit a good thermal and chemical stability, which makes them excellent candidates for field emitter arrays. We present a method that allows for the direct synthesis of nanowire-like ZnO field emitters on free-standing membranes using a porSiO2 template. Besides the application of porSiO2 for the catalyst-assisted growth of nanostructures and their use as field emission devices, the herein presented general synthesis route for the preparation of low refractive index films on other than bulk substrates—such as on free-standing, ultra-thin membranes—may pave the way for the employment of porSiO2 in micro-electro-mechanical systems.
A Comparison of EIS and QCM NanoMIP-Based Sensors for Morphine
Authors: D’Aurelio Roberta , Tothill Ibtisam E. , Salbini Maria , Calò Francesca , Mazzotta Elisabetta , Malitesta Cosimino , Chianella Iva
Published: December 2021

In this work we have compared two different sensing platforms for the detection of morphine as an example of a low molecular weight target analyte. For this, molecularly imprinted polymer nanoparticles (NanoMIP), synthesized with an affinity towards morphine, were attached to an electrochemical impedance spectroscopy (EIS) and a quartz crystal microbalance (QCM) sensor. Assay design, sensors fabrication, analyte sensitivity and specificity were performed using similar methods. The results showed that the EIS sensor achieved a limit of detection (LOD) of 0.11 ng·mL−1, which is three orders of magnitude lower than the 0.19 µg·mL−1 achieved using the QCM sensor. Both the EIS and the QCM sensors were found to be able to specifically detect morphine in a direct assay format. However, the QCM method required conjugation of gold nanoparticles (AuNPs) to the small analyte (morphine) to amplify the signal and achieve a LOD in the µg·mL−1 range. Conversely, the EIS sensor method was labor-intensive and required extensive data handling and processing, resulting in longer analysis times (~30–40 min). In addition, whereas the QCM enables visualization of the binding events between the target molecule and the sensor in real-time, the EIS method does not allow such a feature and measurements are taken post-binding. The work also highlighted the advantages of using QCM as an automated, rapid and multiplex sensor compared to the much simpler EIS platform used in this work, though, the QCM method will require sample preparation, especially when a sensitive (ng·mL−1) detection of a small analyte is needed.
Cation Crosslinking-Induced Stable Copper Nanoclusters Powder as Latent Fingerprints Marker
Authors: Qiu Yi , Wen Zhuoqi , Mei Shiliang , Wei Jinxin , Chen Yuanyuan , Hu Zhe , Cui Zhongjie , Zhang Wanlu , Xie Fengxian , Guo Ruiqian
Published: December 2021

Luminescent copper nanoclusters (Cu NCs) have shown great potential in light-emitting devices (LEDs), chemical sensing, catalysis and biological fields. However, their practical use has been restricted by poor stability, and study on the stability of Cu NCs solid powder along with the mechanism is absent. In this study, stablized Cu NCs powder was first obtained by cation crosslinking method. Compared with the powder synthesized by solvent precipitation method, the stability of Cu NCs powder crosslinked by ionic inducer Ce3+ was enhanced around 100-fold. The storage time when the fluorescence intensity decreased to 85% (T85) was improved from 2 h to 216 h, which is the longest so far. The results of characterizations indicated that the aggregation structure was formed by the binding of Ce3+ with the capping ligands of Cu NCs, which helped in obtaining Ce-Cu NCs powder from aggregate precipitation in solution. Furthermore, this compact structure could avoid the destruction of ambient moisture resulting in long-lasting fluorescence and almost unchanged physical form. This demonstrated that phosphor, with excellent characteristics of unsophisticated synthesis, easy preservation and stable fluorescence, showed great potential in light sources, display technology and especially in latent fingerprints visualization on different substrates for forensic science.
Spark Plasma Sintering of Aluminum Nanocomposite Powders: Recent Strategy to Translate from Lab-Scale to Mass Production
Authors: Hernández-Maya Roberto , Ulloa-Castillo Nicolás Antonio , Martínez-Romero Oscar , Segura-Cárdenas Emmanuel , Elías-Zúñiga Alex
Published: December 2021

The aim of this paper focuses on presenting a recent study that describes the fundamental steps needed to effectively scale-up from lab to mass production parts produced from Al powders reinforced with 0.5 wt% of industrial multiwalled carbon nanotubes (MWCNTs), with mechanical and electrical conductivity properties higher that those measured at the lab scale. The produced material samples were produced via a Spark Plasma Sintering (SPS) process using nanocomposite aluminum powders elaborated with a planetary ball-mill at the lab scale, and high-volume attrition milling equipment in combination with controlled atmosphere sinter hardening furnace equipment, which were used to consolidate the material at the industrial level. Surprisingly, the electrical conductivity and mechanical properties of the samples produced with the reinforced nanocomposite Al powders were made with mass production equipment and were similar or higher than those samples fabricated using metallic powders prepared with ball-mill lab equipment. Experimental measurements show that the hardness and the electrical conductivity properties of the samples fabricated with the mass production Al powders are 48% and 7.5% higher than those of the produced lab samples. This paper elucidates the steps that one needs to follow during the mass production process of reinforced aluminum powders to improve the physical properties of metallic samples consolidated via the SPS process.
Application of Selected Biomaterials and Stem Cells in the Regeneration of Hard Dental Tissue in Paediatric Dentistry—Based on the Current Literature
Authors: Wrzyszcz-Kowalczyk Alina , Dobrzynski Maciej , Grzesiak-Gasek Iwona , Zakrzewski Wojciech , Mysiak-Debska Monika , Nowak Patrycja , Zimolag Malgorzata , Wiglusz Rafal J.
Published: December 2021

Currently, the development of the use of biomaterials and their application in medicine is causing rapid changes in the fields of regenerative dentistry. Each year, new research studies allow for the discovery of additional possibilities of dental tissue restoration. The structure and functions of teeth are complex. They consist of several diverse tissues that need to act together to ensure the tooth’s function and durability. The integrity of a tooth’s enamel, dentin, cementum, and pulp tissue allows for successful mastication. Biomaterials that are needed in dentistry must withstand excessive loading forces, be biocompatible with the hosts’ tissues, and stable in the oral cavity environment. Moreover, each tooth’s tissue, as well as aesthetic qualities in most cases, should closely resemble the natural dental tissues. This is why tissue regeneration in dentistry is such a challenge. This scientific research focuses on paediatric dentistry, its classification of caries, and the use of biomaterials in rebuilding hard dental tissues. There are several methods described in the study, including classical conservative methods such as caries infiltration or stainless-steel crowns. Several clinical cases are present, allowing a reader to better understand the described methods. Although the biomaterials mentioned in this work are artificial, there is currently ongoing research regarding clinical stem cell applications, which have a high potential for becoming one of the most common techniques of lost dental-tissue regeneration in the near future. The current state of stem cell development is mentioned, as well as the various methods of its possible application in dentistry.
Nutrigenomic Studies on the Ameliorative Effect of Enzyme-Digested Phycocyanin in Alzheimer’s Disease Model Mice
Authors: Imai Yasuyuki , Koseki Yurino , Hirano Makoto , Nakamura Shin
Published: December 2021

Alzheimer’s disease (AD) is the most common form of dementia, and the cognitive impairments associated with this degenerative disease seriously affect daily life. Nutraceuticals for the prevention or delay of AD are urgently needed. It has been increasingly observed that phycocyanin (PC) exerts neuroprotective effects. AD model mice intracerebroventricularly injected with amyloid beta-peptide 25–35 (Aβ25–35) at 10 nmol/head displayed significant cognitive impairment in the spontaneous alternation test. Cognitive impairment was significantly ameliorated in mice treated with 750 mg/kg of enzyme-digested (ED) PC by daily oral administration for 22 consecutive days. Application of DNA microarray data on hippocampal gene expression to nutrigenomics studies revealed that oral EDPC counteracted the aberrant expression of 35 genes, including Prnp, Cct4, Vegfd (Figf), Map9 (Mtap9), Pik3cg, Zfand5, Endog, and Hbq1a. These results suggest that oral administration of EDPC ameliorated cognitive impairment in AD model mice by maintaining and/or restoring normal gene expression patterns in the hippocampus.