bluuebunny/crossref_metadata_2025 huggingface dataset:
Simulated galactic cosmic ray exposure activates dose-dependent DNA repair response and down regulates glucosinolate pathways in arabidopsis seedlings
Authors: Dixit Anirudha R. , Meyers Alexander D. , Richardson Brian , Richards Jeffrey T. , Richards Stephanie E. , Neelam Srujana , Levine Howard G. , Cameron Mark J. , Zhang Ye
Published: December 2023

Outside the protection of Earth’s magnetic field, organisms are constantly exposed to space radiation consisting of energetic protons and other heavier charged particles. With the goal of crewed Mars exploration, the production of fresh food during long duration space missions is critical for meeting astronauts’ nutritional and psychological needs. However, the biological effects of space radiation on plants have not been sufficiently investigated and characterized. To that end, 10-day-old Arabidopsis seedlings were exposed to simulated Galactic Cosmic Rays (GCR) and assessed for transcriptomic changes. The simulated GCR irradiation was carried out in the NASA Space Radiation Laboratory (NSRL) at Brookhaven National Lab (BNL). The exposures were conducted acutely for two dose points at 40 cGy or 80 cGy, with sequential delivery of proton, helium, oxygen, silicon, and iron ions. Control and irradiated seedlings were then harvested and preserved in RNAlater at 3 hrs post irradiation. Total RNA was isolated for transcriptomic analyses using RNAseq. The data revealed that the transcriptomic responses were dose-dependent, with significant upregulation of DNA repair pathways and downregulation of glucosinolate biosynthetic pathways. Glucosinolates are important for plant pathogen defense and for the taste of a plant, which are both relevant to growing plants for spaceflight. These findings fill in knowledge gaps of how plants respond to radiation in beyond-Earth environments.
Effect of continuous planting on Casuarina equisetifolia rhizosphere soil physicochemical indexes, microbial functional diversity and metabolites
Authors: Wang Yuhua , Li Jianjuan , Li Mingzhe , Jia Xiaoli , Cai Yuhong , Hu Mingyue , Zhang Qingxu , Cheng Pengyuan , Lin Shaoxiong , Lin Wenxiong , Wang Haibin , Wu Zeyan
Published: December 2023

Continuous planting has a severe impact on the growth of Casuarina equisetifolia. In this study, the effects of three different long-term monocultures (one, two and three replanting) on the physicochemical indexes, microbial functional diversity, and soil metabolomics were analyzed in C. equisetifolia rhizosphere soil. The results showed that rhizosphere soil organic matter content, cation exchange capacity, total and available nitrogen, total and available phosphorus, and total and available potassium contents significantly decreased with the increasing number of continuous plantings. The evaluation of microbial functional diversity revealed a reduction in the number of soil microorganisms that rely on carbohydrates for carbon sources and an increase in soil microorganisms that used phenolic acid, carboxylic acid, fatty acid, and amines as carbon sources. Soil metabolomics analysis showed a significant decrease in soil carbohydrate content and a significant accumulation of autotoxic acid, amine, and lipid in the C. equisetifolia rhizosphere soil. Consequently, the growth of C. equisetifolia could hinder total nutrient content and their availability. Thus, valuable insights for managing the cultivation of C. equisetifolia and soil remediation were provided.
Net CO2 assimilation rate response of tomato seedlings (Solanum lycopersicum L.) to the interaction between light intensity, spectrum and ambient CO2 concentration
Authors: Moratiel Rubén , Jimenez Raúl , Mate Miriam , Ibánez Miguel Angel , Moreno Marta M. , Tarquis Ana M.
Published: December 2023

Artificial lighting is complementary and single-source lighting for controlled Environment Agriculture (CEA) to increase crop productivity. Installations to control CO2 levels and luminaires with variable spectrum and intensity are becoming increasingly common. In order to see the net assimilation of CO2 based on the relationship between the three factors: intensity, spectrum and CO2 concentration, tests are proposed on tomatoes seedling with combinations of ten spectra (100B, 80B20G, 20B80G, 100G, 80G20R, 20G80R, 100R, 80R20B, 20R80B, 37R36G27B) seven light intensities (30, 90, 200, 350, 500, 700 and 1000 μmol·m-2 s-1) and nine CO2 concentrations (200, 300, 400, 500, 600, 700, 800 and 900 ppm). These tomato seedlings grew under uniform conditions with no treatments applied up to the moment of measurement by a differential gas analyzer. We have developed a model to evaluate and determine under what spectrum and intensity of light photosynthesis the Net assimilation of CO2 (An) is more significant in the leaves of tomato plants, considering the CO2 concentration as an independent variable in the model. The evaluation of the model parameters for each spectrum and intensity shows that the intensity has a more decisive influence on the maximum An rate than the spectra. For intensities lower than 350 μmol·m-2 s-1, it is observed that the spectrum has a greater influence on the variable An. The spectra with the best behaviour were 80R20B and 80B20R, which maintained An values between 2 and 4 (μmol CO2·m-2·s-1) above the spectra with the worst behaviour (100G, 80G20R, 20G80R and 37B36G27R) in practically all situations. Photosynthetic Light-Use Efficiency (PLUE) was also higher for the 80B20R and 20R80B spectra with values of 36,07 and 33,84 mmol CO2·mol photon-1, respectively, for light intensities of 200 μmol·m-2 s-1 and 400 ppm of CO2that increased to values of 49,65 and 48,38 mmol CO2·mol photon-1 for the same light intensity and concentrations of 850 ppm. The choice of spectrum is essential, as indicated by the data from this study, to optimize the photosynthesis of the plant species grown in the plant factory where light intensities are adjusted for greater profitability.
Confirmatory factor analysis and gender invariance of the Persian version of psychological control scale: association with internalizing and externalizing behavior problems
Authors: Habibi Asgarabad Mojtaba , Salehi Yegaei Pardis , Mokhtari Sima , Izalnoo Balal , Trejos-Castillo Elizabeth
Published: December 2023

Introduction

The current cross-sectional study aimed to examine the reliability, construct validity, gender invariance and concurrent validity of the psychological control scale-youth self-report (PCS-YSR) among Iranian adolescents.

Methods

A total of 1,453 high school students (49.2% boys; Mage = 15.48, SD = 0.97), who aged between 14 and 18 years old completed the PCS-YSR and the youth self-report (YSR) scale of behavior problems.

Results

Reliability was established using Cronbach’s alpha and ordinal alpha for maternal and paternal psychological control. The confirmatory factor analysis (CFA) results supported the original unidimensional model of the PCS-YSR scale for both mother and father forms. Results also revealed that mother and father forms of PCS-YSR were invariant across adolescents’ gender. When comparing the mean differences, mothers were more psychologically controlling toward their sons, compared to their daughters. The mother and father forms of PCS-YSR were found to have acceptable concurrent validity through their relationship to internalizing and externalizing behavioral problems.

Discussion

Overall, our findings supported the psychometric properties of the Persian version of the Psychological Control Scale-Youth Self-Report among Iranian adolescents. This scale can be used as an efficient tool for parental psychological control among adolescents in Iran. The negative effect of the intrusive parenting behavior on child’ negative outcomes in Iran, irrespective of culture, was shown.
Outlining a novel psychometric model of mental flexibility and affect dynamics
Authors: Borghesi Francesca , Chirico Alice , Cipresso Pietro
Published: December 2023

Theoretically, affective states have always been conceived as complex phenomena enabling individuals to respond flexibly and dynamically to environmental demands. Methodologically, the novel field of Affect Dynamics has started to analyze affective states as inherently dynamic and interdependent phenomena by focusing on how and why they fluctuate over time. Fluctuations of affective states can also be conceived as a function of individuals’ ability to flexibly modulate their responses according to environmental demands. However, this ability has been sparsely investigated in different disciplines and domains, thus, engendering a plethora of terms and models. In this conceptual analysis, we first aimed to disentangle the puzzle of flexibility by outlining the distinctive cross-domain features of this concept, thus providing a novel comprehensive operationalization. We termed this novel unitary concept “mental flexibility,” the general ability to variably adapt to environmental demands. Then, we outlined the interplay between individuals’ mental flexibility and affect dynamics by proposing a novel psychometric model of affect dynamics, using Markovian chain.
Mitochondrial DNA oxidation, methylation, and copy number alterations in major and bipolar depression
Authors: Ceylan Deniz , Karacicek Bilge , Tufekci Kemal Ugur , Aksahin Izel Cemre , Senol Sevin Hun , Genc Sermin
Published: December 2023

Background

Mood disorders are common disabling psychiatric disorders caused by both genetic and environmental factors. Mitochondrial DNA (mtDNA) modifications and epigenetics are promising areas of research in depression since mitochondrial dysfunction has been associated with depression. In this study we aimed to investigate the mtDNA changes in depressive disorder (MDD) and bipolar disorder (BD).

Methods

Displacement loop methylation (D-loop-met), relative mtDNA copy number (mtDNA-cn) and mtDNA oxidation (mtDNA-oxi) were investigated in DNA samples of individuals with MDD (n = 34), BD (n = 23), and healthy controls (HC; n = 40) using the Real-Time Polymerase Chain Reaction (RT-PCR). Blood samples were obtained from a subset of individuals with MDD (n = 15) during a depressive episode (baseline) and after remission (8th week).

Results

The study groups exhibited significant differences in D-loop-met (p = 0.020), while relative mtDNA-cn and mtDNA-oxi showed comparable results. During the remission phase (8th week), there were lower levels of relative mtDNA-cn (Z = −2.783, p = 0.005) and D-loop-met (Z = −3.180, p = 0.001) compared to the acute MDD baseline, with no significant change in mtDNA-oxi levels (Z = −1.193, p = 0.233).

Conclusion

Our findings indicate significantly increased D-loop methylation in MDD compared to BD and HCs, suggesting distinct mtDNA modifications in these conditions. Moreover, the observed alterations in relative mtDNA-cn and D-loop-met during remission suggest a potential role of mtDNA alterations in the pathophysiology of MDD. Future studies may provide valuable insights into the dynamics of mtDNA modifications in both disorders and their response to treatment.
Cross-sectional study on the dissociation of decision-making capacity for antipsychotic treatment and COVID-19 vaccination in individuals with schizophrenia
Authors: Raffard Stéphane , Bayard Sophie , Tattard Philippe , Laraki Yasmine , Capdevielle Delphine
Published: December 2023

Objective

Decision-making capacity for treatment is impaired in schizophrenia but it remains unknown if schizophrenia affects distinct decision-making capacities differently.

Methods

In this study, we assessed concomitantly two decision-making capacities (i.e., antipsychotic treatment and COVID-19 vaccination) in 27 schizophrenia patients. Sociodemographic variables, psychotic symptoms, global cognition and insight were also assessed.

Results

We found that among individuals incompetent to consent to antipsychotic treatment, one-third had preserved capacity to consent to vaccination. No significant associations between the two different decision-making capacities were found. Psychotic symptoms and cognition were associated with the capacity to consent to antipsychotic treatment and to vaccination, respectively.

Conclusion

Clinicians should be aware that capacity to consent to treatment is not unidimensional and vary across domains in individuals with schizophrenia. Being incompetent regarding one treatment does not mean to be incompetent for another treatment in this clinical population.
Daily fluctuations in COVID-19 infection rates under Tokyo’s epidemic prevention measures – new evidence from adaptive Fourier decomposition
Authors: Lu Guibin , Yang Zifeng , Qu Wei , Qian Tao , Liu Zige , He Wei , Lin Zhijie , Hon Chitin
Published: December 2023

Background

The COVID-19 pandemic has witnessed widespread infections and variants. Particularly, Tokyo faced the challenge of seven waves of COVID-19, during which government interventions played a pivotal role. Therefore, gaining a comprehensive understanding of government control measures is of paramount importance, which is beneficial for health authorities in the policy development process.

Method

Our study analysis the daily change data of the daily COVID-19 infection count in Tokyo from January 16, 2020 to September 30, 2022. We utilized adaptive Fourier decomposition (AFD) for analyzing the temporal trends within COVID-19 data. It extends the conventional AFD approach by constructing new components base on multiple individual components at various time-frequency scales. Furthermore, we conducted Pearson correlation assessments of the first to third-order synthesis results, along with comparative analyses against other signal analysis techniques. Ultimately, these new components are integrated with policy data spanning different time periods for a comprehensive analysis.

Result

The analysis of daily COVID-19 data in Tokyo using AFD reveals how various government policies impacted infection rates across seven distinct fluctuation periods. In the decomposition results, the reduction of business hours policy correlated with high-frequency components in the first four waves, while the low-frequency components for the sixth wave suggested a decline in its relevance. The vaccination policy initially displayed a mid-frequency correlation with the fifth wave and continued with a low-frequency correlation in the last wave. Moreover, our statistical analysis (value of p < 0.05) demonstrated that 75% of the third-order AFD components exhibited significant positive correlations with the original infections, while the correlation coefficients of most components in EMD and VMD did not attain significance.

Conclusion

In the time-frequency domain, AFD demonstrates superior performance compared to EMD and VMD in capturing crucial data related to epidemic control measures. The variations in daily COVID-19 infection counts during these seven periods under various policies are evident in distinct third-order AFD components. These findings guide the formulation of future public health policies and social measures.
“They don't care about people; they only care about the money”: the effects of border enforcement, commodification and migration industries on the mobility of migrants in transit through Mexico
Authors: Willers Susanne
Published: December 2023

How does border enforcement affect the mobility of migrants and refugees in countries of transit? What impact does it have on migrants' bodily experiences of mobility and their reliance on actors of the migration industry? While the externalization of borders affects undocumented people by increasing their vulnerability to violence during transit, the impact of the migration regime on the social construction of inequalities in every-day interactions and its relationship to the capacity for mobility has not been studied in depth. This article intends to bridge this gap: based on ethnographic fieldwork I conducted between 2013 and 2019, this article analyzes the relation between immigration enforcement and the mobility strategies of migrants and refugees, particularly women. It focuses on the intertwining of border enforcement and violence and their impact on people's bodily mobility experiences in transit through Mexico along intersecting lines of inequality such as race, class, gender and nationality. First, I analyze how border enforcement contributes to internal bordering, thereby increasing the vulnerability and dependence of migrants on brokers for mobility; second, it looks at the bodily experiences of women in transit and the ways in which internal bordering shapes gendered power hierarchies among actors in the field of mobility. The analysis shows how women negotiate mobility and bodily integrity in social interactions with different actors and how they face constraints resulting from the gendered hierarchies to mobility on routes of transit. Furthermore, it demonstrates how women's bodies have become a privileged site for the construction of a 'body politic' exploitable by others, since border enforcement has contributed to weakening the possibilities of negotiating mobility and bodily integrity in transit.
Associations between load-velocity profiling and race parameters of elite swimmers in the 100 and 200m freestyle events
Authors: Raineteau Yannis , Nicolas Guillaume , Bideau Benoit , Bideau Nicolas , Pla Robin
Published: December 2023

Introduction

Improving swimming performance involves assessments of biomechanical variables of the stroke, and it can be achieved using semi-tethered swimming tests. The aim of this study was thus to investigate the associations between load-velocity (L-V) profiles, from a semi-tethered swimming protocol and race variables in the 100 m and 200 m freestyle events.

Methods

Eight swimmers completed a L-V profiling protocol consisting of four sprints (25 m, 25 m, 20 m, 15 m) against increasing loads (0.1, 2.0, 4.0, 6.0 kg respectively) with complete recovery between repetitions (>5 min). The L-V linear regression was used to estimate maximal velocity (V0) and body mass normalized load (rL0). Race variables such as clean swimming speed (V), stroke rate (SR), distance per cycle (SL) and stroke index (SI) were assessed from video analysis of 100 m and 200 m freestyle events taking place 3–4 days after the L-V protocol.

Results

L-V results showed high levels of speed (mean ± SD: 1.87 ± 0.04 m/s) and heavy maximal relative loads (mean ± SD: 38.5 ± 6.51 as % of body mass). Swimmers also achieved high-level performances in the 100 m (mean ± SD time: 51.95 ± 0.75 s) and the 200 m (mean ± SD time: 113.85 ± 2.67 s). For the 100 m events, the maximal relative load showed strong correlation with performance (r = 0.63) whereas trivial correlation was observed for the 200 m events (r = 0.12). SR on the 100 m and the 200 m also showed very strong association with rL0 (r = 0.83) and a strong association with V0 (r = 0.68) respectively.

Conclusion

The relationships between L-V variables and race variables depend on the distance of the event. However, L-V variables seem to be less related to SR and SL evolutions for the 100 m than in the 200 m event. Moreover, L-V profiles tend to be more related to the 100 m than 200 m freestyle performance. L-V profile should be interpreted taking into consideration the specific physiological and biomechanical constraints of the main events of the swimmer.
Exploring sustainable food choices among adults in the United Arab Emirates: a cross-sectional study
Authors: Cheikh Ismail Leila , Hashim Mona , Osaili Tareq M. , Faris MoezAlIslam E. , Naja Farah , Radwan Hadia , Hasan Fayeza , Saleh Sheima T. , Al Rajaby Radhiya , Al Daour Rameez , Stojanovska Lily , Al Dhaheri Ayesha S. , Hasan Hayder , Obaid Reyad Shaker
Published: December 2023

Introduction

Dietary choices serve as a vital and ongoing link between environmental sustainability and human health. This study aimed to assess the consumption behavior of people in the United Arab Emirates (UAE) concerning sustainability and determine factors that contribute to sustainable food choices.

Methods

A cross-sectional, web-based study was conducted among adults in the UAE (n = 1,113). Data on participants’ sociodemographic characteristics, attitudes toward sustainable food choices, and reasons behind sustainable food choices were collected. Independent t-test and one-way ANOVA tests were used to investigate the differences in the level of agreement to nine statements about sustainable food choices among different sociodemographic groups on a scale of 1–5 ranging from strongly disagree to strongly agree. Participants’ responses to open-ended questions were filtered and clustered into eight different categories and presented as counts and percentages.

Results and discussion

Participants agreed on trying new healthy and environmentally friendly foodstuff (3.57 ± 1.04). Females (p = 0.002) and older adults (>50 years; p = 0.001) showed higher agreement with avoiding red meat. Older participants, and those with higher education (p = 0.020 and p < 0.001, respectively) showed higher agreement with favoring plant-based diets. 21.2% reported avoiding red meat and 23.1% preferred a plant-based diet. Of those who favored plant-based food the main reported reason was ‘health and nutritive value’ (66.5%), while of those who avoided red meat, preference was the most reported reason (41.9%). Although the study sample did not greatly adopt sustainable food choices, they leaned toward trying environmentally friendly foods. Females, older adults, and highly educated people aligned themselves with more sustainable food choices. Targeted policies and the integration of sustainability aspects within dietary guidelines to promote healthy, sustainable, and affordable diets are needed.
Interhospital Spread of blaVIM-1- and blaCTX-M-15-Producing K. pneumoniae ST15 on an IncR Plasmid in Southern Spain
Authors: Pérez-Palacios Patricia , Gual-de-Torrella Ana , Portillo-Calderón Ines , Recacha-Villamor Esther , Franco-Álvarez de Luna Francisco , Lopez-Cerero Lorena , Pascual Alvaro
Published: December 2023

In 2014–2015, the main CTX-M-15- and OXA-48-producing clone in our region was ST15. Recently, K. pneumoniae ST15 isolates co-producing VIM-1 and CTX-M-15 were detected in several hospitals. The aim was to study the emergence and acquisition of this carbapenemase. Between 2017 and 2019, four hospitals submitted twenty-nine VIM-1- and CTX-M-15-producing K. pneumoniae ST15 isolates to our laboratory. Seven representatives of each XbaI PFGE pulsotype were sequenced using short- and long-read technologies. RAST, CGE databases, and Pathogenwatch were used for resistance determinants and capsule-type analysis. Plasmid comparison was performed with Easyfig2.1. Phylogenetic analysis included other contemporary ST15 isolates from Spain. The 29 isolates were clustered into seven different pulsotypes. The selected genomes, from three hospitals in two different provinces, were clustered together (fewer than 35 alleles) and differed by more than 100 alleles from other ST15 isolates obtained in the region. These seven isolates harbored one IncR plasmid (200–220 kb) with a common backbone and four regions flanked by IS26: one contained blaVIM-1, another contained blaCTX-M-15, the third contained blaOXA-1, and the fourth harbored heavy-metal-tolerance genes. The two initial plasmids, from two different centers, were identical, and rearrangement of four regions was observed in the five subsequent plasmids. Our findings showed the first intercenter dissemination of IncR plasmids carrying blaVIM-1, blaCTX-M-15, and metal-tolerance genes mediated by a new lineage of K. pneumoniae ST15. Two different capture events of the blaVIM-1 gene or different IS26-mediated plasmid rearrangements from a common ancestor may explain plasmid variations.
Reply to Papini, A. Comment on “Nekkaa et al. Rhamnus alaternus Plant: Extraction of Bioactive Fractions and Evaluation of Their Pharmacological and Phytochemical Properties. Antioxidants 2021, 10, 300”
Authors: Nekkaa Amine , Benaissa Akila , Mutelet Fabrice , Canabady-Rochelle Laetitia
Published: December 2023

We appreciate the commentary by Alessio Papini [...]
Bioactives and Technological Quality of Functional Biscuits Containing Flour and Liquid Extracts from Broccoli By-Products
Authors: Fanesi Benedetta , Ismaiel Lama , Nartea Ancuta , Orhotohwo Oghenetega Lois , Kuhalskaya Anastasiya , Pacetti Deborah , Lucci Paolo , Falcone Pasquale Massimiliano
Published: December 2023

Broccoli by-products are an important source of health-promoting bioactive compounds, although they are generally underutilized. This study aimed to valorize non-compliant broccoli florets by transforming them into functional ingredients for biscuit formulation. A broccoli flour and three water/ethanol extracts (100:0, 75:25, 50:50; v/v) were obtained. The rheological properties and the content of bioactive compounds of the functional ingredients and biscuits were evaluated. The 50:50 hydroalcoholic extract was the richest in glucosinolates (9749 µg·g−1 DW); however, the addition of a small amount strongly affected dough workability. The enrichment with 10% broccoli flour resulted the best formulation in terms of workability and color compared to the other enriched biscuits. The food matrix also contributed to protecting bioactive compounds from thermal degradation, leading to the highest total glucosinolate (33 µg·g−1 DW), carotenoid (46 µg·g−1 DW), and phenol (1.9 mg GAE·g−1 DW) contents being present in the final biscuit. Therefore, broccoli flour is a promising ingredient for innovative healthy bakery goods. Hydroalcoholic extracts could be valuable ingredients for liquid or semi-solid food formulation.
Experimental and Numerical Evaluation of a Wildland–Urban Interface Fire Scenario
Authors: Craveiro Hélder D. , Fiorini Cesare , Laím Luís , Guillaume Bruno , Santiago Aldina
Published: December 2023

This paper presents the results obtained from a field fire test, aiming to reproduce a wildland–urban interface scenario to collect relevant information concerning the impact of wildfires on the built environment. The objective was to understand heat transfer mechanisms from forest fires to structures. During the fire test, the temperatures at the exposed face of one building component were monitored, as well as those in the vicinity of that component, using thermal imaging. The detailed characterization of the field test and building component and obtained experimental results of the fire test were then used to develop and validate a complex computational fluid dynamics model (full physics models) using the Fire Dynamics Simulator (FDS). Several numerical models were previously developed to reproduce the behaviour of individual shrubs and trees in fires considering available results in the literature. The developed Computational Fluid Dynamics (CFD) models can accurately reproduce the field test, including the fire spread and the temperature evolution on the surface of the exposed construction component. The obtained maximum temperature in the construction element was 1038 °C, whereas the maximum average temperature was approximately 638 °C. According to the results from the numerical model, the construction element was exposed to a very high heat flux (above 40 kW/m2), indicating direct contact of the flames with the construction element. The use of CFD enables the quantification of the characteristics of the fire and the exposure of structures to fire in the wildland–urban interface (WUI), allowing for the definition of a performance-based design approach for buildings in the WUI. This contributes to developing safe and resilient structures, as well as mitigating and reducing the impacts of wildfires in the built environment.
A Study on Exploring the Level of Awareness of Privacy Concerns and Risks
Authors: Nguyen Tommy , Yeates Garnet , Ly Tony , Albalawi Umar
Published: December 2023

With the recent increase in phishing attacks and other kinds of malicious activities, increasing the awareness of security and privacy awareness is arguably one of the best proven ways of preventing these kinds of threats. The main challenge in security and privacy awareness is the end user’s awareness of aspects of privacy and security they give up when using the Internet. Thus, this study focuses on identifying and discussing the growing threats of Internet usage and the lack of privacy and security knowledge of the everyday person. This study presents the results of a survey conducted to determine discrepancies between what rights users think they sign away when they agree to terms of service versus what rights they actually give away. It is hypothesized that people are genuinely unaware of what rights they are giving up, especially since they are signing the terms of service without reading the fine print. In this study, the terms of service were presented to respondents, and they answered questions on what they thought they were giving up, but once they answered the questions, they were explicitly told whether they truly knew what rights they signed away. The experimental results of this study examine how much knowledge the everyday person lacks with respect to the privacy policies they sign. All of this is ultimately used to examine possible flaws in the system. The experimental results illustrate the results of the survey. Based on the total surveys completed, the average score was 62%. This means that out of 10 clauses described in a given terms of service document or privacy policy, people are truly unaware of at least 4 of these statements.
Neural Network-Based Approach to Detect and Filter Misleading Audio Segments in Classroom Automatic Transcription
Authors: Hewstone Jorge , Araya Roberto
Published: December 2023

Audio recording in classrooms is a common practice in educational research, with applications ranging from detecting classroom activities to analyzing student behavior. Previous research has employed neural networks for classroom activity detection and speaker role identification. However, these recordings are often affected by background noise that can hinder further analysis, and the literature has only sought to identify noise with general filters and not specifically designed for classrooms. Although the use of high-end microphones and environmental monitoring can mitigate this problem, these solutions can be costly and potentially disruptive to the natural classroom environment. In this context, we propose the development of a novel neural network model that specifically detects and filters out problematic audio sections in classroom recordings. This model is particularly effective in reducing transcription errors, achieving up to a 96% success rate in filtering out segments that could lead to incorrect automated transcriptions. The novelty of our work lies in its targeted approach for low-budget, aurally complex environments like classrooms, where multiple speakers are present. By allowing the use of lower-quality recordings without compromising analysis capability, our model facilitates data collection in natural educational settings and reduces the dependency on expensive recording equipment. This advancement not only demonstrates the practical application of specialized neural network filters in challenging acoustic environments but also opens new avenues for enhancing audio analysis in educational research and beyond.
Study on Seasonal Characteristics and Causes of Marine Heatwaves in the South China Sea over Nearly 30 Years
Authors: Gao Zhenli , Jia Wentao , Zhang Weimin , Wang Pinqiang
Published: December 2023

Marine heatwaves (MHWs) are becoming more frequent and intense in many regions around the world, as well as in China’s marginal seas. However, the seasonal characteristics and associated physical drivers of MHWs are largely unknown. In this study, we analyze, based on multiple reanalysis and numerical model data, the seasonal characteristics and causes of MHWs in the South China Sea (SCS) over a near 30-year period (1991–2022). There exist significant seasonal variabilities in the spatiotemporal features and formation mechanisms of MHWs. MHWs in the SCS show significant increasing trends in terms of frequency, duration, and intensity. MHWs during the summer half-year are stronger than the winter half-year as a whole, with them being more likely to occur over the eastern SCS in the summer half-year and the western region in the winter half-year. However, the increasing trend of MHWs in the winter half-year exceed those in the summer. Additionally, we find that MHWs are associated with the unusually strong west Pacific subtropical high (WPSH) both in the summer and winter half-years. Nevertheless, the dominant factors for MHWs are different in the varied seasons. According to upper ocean temperature equation analysis, surface heat flux anomalies (especially shortwave radiation flux) are major effect factors in the summer half-year, while ocean dynamic processes play the main role in the winter half-year. An analysis of the typical MHWs also proves this conclusion. Moreover, MHWs occurring in winter are often accompanied by temperature anomalies within the mixed-layer depth. The findings imply that the formation mechanisms and space–time distribution of MHWs exist with a seasonal contrast in the SCS, rather than simply being due to large-scale circulation and flux anomalies. This may provide a useful reference for a deeper understanding and forecasting of MHWs under different seasons and weather.
Repurposing an Antioxidant to Kill Mycobacterium tuberculosis by Targeting the 50S Subunit of the Ribosome
Authors: Dong Wenqi , Wang Gaoyan , Bai Yajuan , Li Yuxin , Zhao Liying , Lu Wenjia , Wang Chenchen , Zhang Zhaoran , Lu Hao , Wang Xiangru , Chen Huanchun , Tan Chen
Published: December 2023

Tuberculosis and drug-resistant TB remain serious threats to global public health. It is urgent to develop novel anti-TB drugs in order to control it. In addition to redesigning and developing new anti-TB drugs, drug repurposing is also an innovative way to develop antibacterial drugs. Based on this method, we discovered SKQ-1 in the FDA-approved drug library and evaluated its anti-TB activity. In vitro, we demonstrated that SKQ-1 engaged in bactericidal activity against drug-sensitive and -resistant Mtb and confirmed the synergistic effects of SKQ1 with RIF and INH. Moreover, SKQ-1 showed a significant Mtb-killing effect in macrophages. In vivo, both the SKQ-1 treatment alone and the treatment in combination with RIF were able to significantly reduce the bacterial load and improve the survival rate of G. mellonella infected with Mtb. We performed whole-genome sequencing on screened SKQ-1-resistant strains and found that the SNP sites were concentrated in the 50S ribosomal subunit of Mtb. Furthermore, we proved that SKQ-1 can inhibit protein translation. In summary, from the perspective of drug repurposing, we discovered and determined the anti-tuberculosis effect of SKQ-1, revealed its synergistic effects with RIF and INH, and demonstrated its mechanism of action through targeting ribosomes and disrupting protein synthesis, thus making it a potential treatment option for DR-TB.
Bioassays for Identifying and Characterizing Plant Regulatory Peptides
Authors: Skripnikov Alexander
Published: December 2023

Plant peptides are a new frontier in plant biology, owing to their key regulatory roles in plant growth, development, and stress responses. Synthetic peptides are promising biological agents that can be used to improve crop growth and protection in an environmentally sustainable manner. Plant regulatory peptides identified in pioneering research, including systemin, PSK, HypSys, RALPH, AtPep1, CLV3, TDIF, CLE, and RGF/GLV/CLEL, hold promise for crop improvement as potent regulators of plant growth and defense. Mass spectrometry and bioinformatics are greatly facilitating the discovery and identification of new plant peptides. The biological functions of most novel plant peptides remain to be elucidated. Bioassays are an essential part in studying the biological activity of identified and putative plant peptides. Root growth assays and cultivated plant cell cultures are widely used to evaluate the regulatory potential of plant peptides during growth, differentiation, and stress reactions. These bioassays can be used as universal approaches for screening peptides from different plant species. Development of high-throughput bioassays can facilitate the screening of large numbers of identified and putative plant peptides, which have recently been discovered but remain uncharacterized for biological activity.
The Role of Pentraxin 3 in Gastrointestinal Cancers
Authors: Zajkowska Monika , Mroczko Barbara
Published: December 2023

Gastrointestinal cancers have become a huge problem worldwide as the number of new cases continues to increase. Due to the growing need to explore new biomarkers and therapeutic targets for the detection and treatment of cancerous lesions, we sought to elucidate the role of Pentraxin-3 in the progression of cancerous lesions, as it is involved in the process of angiogenesis and inflammation. Statistically significant changes in the concentration of this parameter have emerged in many gastrointestinal cancer patients. Moreover, it is related to the advancement of cancer, as well as processes leading to the development of those changes. In the case of studies concerning tissue material, both increased and decreased tissue expression of the tested parameter were observed and were dependent on the type of cancer. In the case of cell lines, both human and animal, a significant increase in Pentraxin 3 gene expression was observed, which confirmed the changes observed at the protein level. In conclusion, it can be assumed that PTX3, both at the level of gene expression and protein concentrations, is highly useful in the detection of gastrointestinal cancers, and its use as a biomarker and/or therapeutic target may be useful in the future.
Co-Encapsulation of Rhenium and Ruthenium Complexes into the Scaffolds of Metal–Organic Framework to Promote CO2 Reduction
Authors: Su Zhifang , Yu Baolan , Feng Jianxin , Zhong Maoling , Li Xuan , Shi Jianying
Published: December 2023

The molecular complexes of Re(4,4′-dcbpy)(CO)3Cl (dcbpy = dicarboxylicacid-2,2’-bipyridyl) and [Ru(dcbpy)3]2+ are co-assembled into UiO-66 scaffolds as structural imperfects for CO2 photocatalytic reduction (named as Re-Ru@U). The prepared catalysts are characterized by XRD, Fourier-Transform infrared (FTIR) spectra, X-ray photoelectron spectra (XPS) and N2 adsorption–desorption isotherms. The intact structure of molecular complexes within the matrix are monitored by 1H nuclear magnetic resonance (NMR) spectra through a totally digesting catalyst. The optical properties are studied via absorption and photoluminescence spectra, and the single-electron reduction in Re and Ru complexes is detected by electron paramagnetic resonance (EPR) spectra. An excellent photocatalytic performance is obtained with steady and sustained CO evolution and a turnover number (TON) value of 15 (11 h). The CO activity irradiating by single wavelength presents the absorption-intensity-dependent changing tendency, where the absorption intensity is superposed by Re and Ru complexes. The two radicals related to Re and Ru, respectively, are simultaneously detected in the Re-Ru@U catalyst. It is suggested that the ReC2 component serves as both a photosensitizer and a catalyst, and the RuC2 component works as an additional photosensitizer to supply the second electron for CO2 reduction. The co-assembling of dual metals Re and Ru in the matrix promotes the electron transfer from the reductive Ru centres to one-electron-reduced Re centres and accounts for the superior activity of CO evolution. Our results demonstrate a strategy to develop the multimetallic catalysts via facile assembling into MOF scaffolds to promote photocatalytic performance.
The Impact of Topological States on the Thermoelectric Performance of p- and n-Type Sb2Te3/Bi2Se3-Multiwalled Carbon Nanotubes Heterostructured Networks
Authors: Andzane Jana , Buks Krisjanis , Spalva Elmars , Felsharuk Andrei , Erts Donats
Published: December 2023

The resistance and magnetoresistance of flexible thermoelectric p-type Sb2Te3-MWCNT, p-type Bi2Se3-MWCNT, and n-type Bi2Se3-MWCNT heterostructures were studied in the temperature range from 2 K to 300 K to reveal the conductance mechanisms governing the thermoelectric properties of these heterostructured networks. It was found that the conductance in heterostructured networks at different temperatures is governed by different processes and components of the networks. This effect was found to be related to the growth mechanisms of the Sb2Te3 and Bi2Se3 nanostructures on the MWCNT networks. At near-room temperatures, the Sb2Te3 and Bi2Se3 nanostructures were found to have the dominant contribution to the total conductance of the p-type Sb2Te3-MWCNT and n-type Bi2Se3-MWCNT networks. In turn, the conduction of p-type Bi2Se3-MWCNT heterostructured networks in a full temperature range and p-type Sb2Te3-MWCNT and n-type Bi2Se3-MWCNT heterostructured networks at temperatures below 30 K was governed by the MWCNTs; however, with the contribution from 2D topological states of Sb2Te3 and Bi2Se3 nanostructures, these were manifested by the weak antilocalization effect (WAL) cusps observed at temperatures below 5–10 K for all heterostructured networks considered in this work.
Mapping Potential Regions of Human Interaction with Acuminate Horseshoe Bats (Rhinolophus acuminatus) in Thailand
Authors: Sirichan Nutthinee , Chaiyes Aingorn , Sánchez Cecilia A. , Wacharapluesadee Supaporn , Srikulnath Kornsorn , Duengkae Prateep
Published: December 2023

Bats are reservoirs for various pathogens, including SARS-like coronaviruses (CoVs). Understanding the distribution of bat species is crucial to identifying areas where viral spillover from bats to other animals or humans might occur. In this study, we performed species distribution modeling to predict suitable habitats within Thailand under current and predicted future climate conditions for Rhinolophus acuminatus, a bat species that has been found to host SARS-CoV-2-related viruses. Our assessment of current conditions revealed that temperature seasonality had the greatest impact on habitat suitability and that suitable habitats were primarily restricted to the southern and eastern regions of Thailand. Over time, the projections indicate a diminishing availability of suitable habitats, suggesting a potential trend toward migration into neighboring areas. We next combined modeled bat distribution with urbanization data to estimate regions in Thailand where bat–human interactions might occur. The resulting map highlighted regions of heightened interaction risk, encompassing approximately 46,053.94 km2 across 58 provinces and representing approximately 9.24% of Thailand’s total area. These risk concentrations are prominently situated in the southern, central, and eastern Thai regions, with extensions into neighboring border areas. Our findings will significantly aid future risk surveillance efforts and enhance the effectiveness of monitoring and managing emerging diseases within the country and in contiguous regions.
Effects of Clothianidin Pesticide Application on the Strength of Honey Bee Colonies and Stress-Related Genes in the Vicinity of Rice Fields in the Republic of Korea
Authors: Son Minwoong , Kim Jisoo , Kim Dongwon , Lee Chang-Hoon , Akongte Peter Njukang , Oh Daegeun , Choi Yong-Soo , Park Bo-Sun
Published: December 2023

The honey bee, a crucial organism that provides ecological and economic value to humans, is currently facing threats from various environmental factors including pesticides. Numerous studies have been conducted to demonstrate the risks associated with neonicotinoid pesticides, but research on their occurrence in actual field conditions has not been identified. Therefore, in this study, we observed changes in honey bee (Apis mellifera) colonies located near rice cultivation areas as they progressed beyond the rice pesticide application period. Furthermore, we collected honey bees exposed to the clothianidin and analyzed their stress-related gene expression. The results showed that the foraging behavior of honey bee colonies located near rice cultivation areas did not exhibit significant differences between the treatment sites (Cheongyang and Gimje) and the control site (Wanju) during the experimental period. However, it was observed that the expression levels of stress-related genes in honey bees collected from the treatment group were significantly higher than those in the control. Most of the stress-related genes were associated with detoxification processes in response to pesticides. As a result, pesticide treatment in proximity to rice cultivation areas did not cause direct damage to honey bees but had an indirect impact, suggesting the potential for ongoing chronic damage.
Review on Pediatric Malignant Focal Liver Lesions with Imaging Evaluation: Part II
Authors: Dong Yi , Cekuolis Andrius , Schreiber-Dietrich Dagmar , Augustiniene Rasa , Schwarz Simone , Möller Kathleen , Nourkami-Tutdibi Nasenien , Chen Sheng , Cao Jia-Ying , Huang Yun-Lin , Wang Ying , Taut Heike , Grevelding Lara , Dietrich Christoph F.
Published: December 2023

Malignant focal liver lesions (FLLs) represent various kinds of epithelial and mesenchymal tumors. In pediatric patients, the understanding of pediatric liver diseases and associated imaging manifestations is essential for making accurate diagnosis and differential diagnosis. This paper will discuss the latest knowledge of the common pediatric malignant FLLs, including undifferentiated embryonal sarcoma, rhabdomyosarcoma, epithelioid hemangioendothelioma, angiosarcoma, and malignant rhabdoid tumor. Medical imaging features are not only helpful for clinical diagnosis, but can also be useful in the evaluation and follow-up of pre- and post-treatment. The future perspectives of contrast-enhanced ultrasound (CEUS) enhancement patterns of FLLs in pediatric patients are also mentioned.
Attenuation Correction of Long Axial Field-of-View Positron Emission Tomography Using Synthetic Computed Tomography Derived from the Emission Data: Application to Low-Count Studies and Multiple Tracers
Authors: Montgomery Maria Elkjær , Andersen Flemming Littrup , d’Este Sabrina Honoré , Overbeck Nanna , Cramon Per Karkov , Law Ian , Fischer Barbara Malene , Ladefoged Claes Nøhr
Published: December 2023

Recent advancements in PET/CT, including the emergence of long axial field-of-view (LAFOV) PET/CT scanners, have increased PET sensitivity substantially. Consequently, there has been a significant reduction in the required tracer activity, shifting the primary source of patient radiation dose exposure to the attenuation correction (AC) CT scan during PET imaging. This study proposes a parameter-transferred conditional generative adversarial network (PT-cGAN) architecture to generate synthetic CT (sCT) images from non-attenuation corrected (NAC) PET images, with separate networks for [18F]FDG and [15O]H2O tracers. The study includes a total of 1018 subjects (n = 972 [18F]FDG, n = 46 [15O]H2O). Testing was performed on the LAFOV scanner for both datasets. Qualitative analysis found no differences in image quality in 30 out of 36 cases in FDG patients, with minor insignificant differences in the remaining 6 cases. Reduced artifacts due to motion between NAC PET and CT were found. For the selected organs, a mean average error of 0.45% was found for the FDG cohort, and that of 3.12% was found for the H2O cohort. Simulated low-count images were included in testing, which demonstrated good performance down to 45 s scans. These findings show that the AC of total-body PET is feasible across tracers and in low-count studies and might reduce the artifacts due to motion and metal implants.