bluuebunny/crossref_metadata_2025 huggingface dataset:
THE SUSTAINABLE DEVELOPMENT LICENSING POLICY OF CREATIVE INDUSTRY IN THE ERA OF ASEAN ECONOMIC COMMUNITY (AEC) IN SURAKARTA, INDONESIA
Authors: . Absori , Nurhayati Nunik , Bangsawan Moh. Indra
Published: March 2019

Purpose of Study: The purpose of this study is to describe the growth of the creative industries in the city of Surakarta, formulate the sustainable development licensing model of creative industries in the era of ASEAN economic community (AEC) through legitimacy as a legal umbrella by designing the local regulation on creative industry.

Methodology: This type of research is a juridical-empirical study with a qualitative non-doctrinal approach. The policy offered is a policy with the Fishbone model analysis which sequentially describes a visual list composed of various causes that affect in process who has done by connecting one cause to another. Each affect will be arranged according to the cause, and aims to classify some causes based on category. The identification process is done by looking for the root cause which influences in irrelevance the sustainable development creative industries legality in the city of Surakarta, namely: Local Government; Regulation; Creative Industry Businessman and the environment who will describe with a small bone. While the big bone is the result of the analysis of the root of the problem of the four indicators mentioned above, which is the root of the problem (the big bone) the irrelevance of the sustainable development creative industries legality in the city of Surakarta.

Results:  Based on the results,  this research showed that there has been an increasing trend of creative industry growth in the city of Surakarta in 2015-2016 which touched the number 495 creative industries. However, the data show that in 2015-2016 only 10% of creative industries have business licenses and 90% not have the business license.

Implications: Therefore, the local government has to make and formulate the local regulations as licence of sustainable development creative industries in the city of Surakarta and designing the guideline book to make easier for the society in order to understand the rules.
Antioxidant, acetylcholinesterase and butyrylcholinesterase inhibition profiles of histamine Schiff bases
Authors: AKOCAK Süleyman , LOLAK Nabih , TUNEG Muhammed , BOGA Mehmet
Published: March 2019

In this work, a series of histamine Schiff bases H(1-20) were synthesized by reacting
histamine and substituted aldehydes A(1-20).
The compounds were assayed for antioxidant properties by using different
bioanalytical methods such as DPPH free radical scavenging assay, ABTS cation
radical decolarization, cupric reducing antioxidant capacity (CUPRAC) and metal
chelating methods. The acetylcholinesterase (AChE) and butyrylcholinesterase
(BChE) inhibition profiles were also assessed. In general, the synthesized
compounds showed weak antioxidant activity against all tested methods, but some
of the compounds showed great inhibition potency against AChE and BChE enzymes.
Specifically, compound H9 showed
effective inhibition potency against both enzymes with % inhibition of 97.03
and 93.64, respectively.
Nature of zircon clastics in the Riphean and Vendian sandstones of the Southern Urals
Authors: Krasnobaev A.A. , Puchkov V.N. , Sergeeva N.D. , Busharina S.V.
Published: March 2019

New age dates of detrital zircons of terrigenous rocks augmented the possibilities of interpretation of their provenance. Unfortunately this interpretation is restricted by a formal comparison of age-and-composition characteristics of detrital crystals with any very distant model objects. The paper deals with a situation when the role of a source of a detritus is claimed by local objects. When comparing the age parameters of primary and detrital crystals of zircons, the data on Riphean volcanics and ancient metamorphics of the Taratash complex of the Southern Urals were used. Specifying the ideas on the nature of the zircon clastics (detritus) and its relationships with primary zircons of sources, a role of processes of mechanical abrasion is pointed out, leading to a clearing of heterogenous primary grains of defect crystals, which results in an accumulation of crystals of more homogenous appearance. The analysis of SHRIMP and TIMS-dates of zircons and U and Th concentrations in them, and also a comparison of histograms of primary zircons from Riphean volcanics and rocks of the Taratash complex on one hand and the detrital zircons from the Vendian and Riphean sandstones of the Southern Urals on the other, have shown that the age variations of both are rather comparable. It means that the age characteristics of primary zircons from the Riphean volcanics and rocks of the Taratash complex as sources of zircon clastics for the Riphean and Vendian sandstones are regulated by processes of resedimentation, and a detrital fraction of zircons is formed at the expense of local objects. The participation of very distant sources is not excluded, but in our case it is not detected.
Research of domanic source rock by pyrolytic gas chromatography-mass spectrometry method
Authors: Chemodanov Artem E. , Gareev Bulat I. , Batalin Georgy A. , Gerasimov Roman S.
Published: March 2019

We applied new novel approach based on pyrolytic gas chromatography-mass spectrometry technology (Py-GCMS) developed by Frontier Lab company (Japan) in order to get the same data as it can be acquired by using conventional core analyzer. Experimental part describes the detailed measurement procedure, temperature program and outcomes acquired by using IFP 160000 as a standard sample. We have managed to demonstrate accuracy and reproducibility of tests for the domanic source rock samples and its kerogen extracted respectively. In results of this analytical challenge we were able to get pyrograms providing the S1 (free hydrocarbons), S2 (potential hydrocarbons), Tmax (temperature at which the maximum rate of hydrocarbon generation is reached) information with level of confidence we usually could see doing conventional core analyzer tests. Some important conclusions regarding petroleum generation and thermal maturity have been made. S1 and S2 significantly decrease for kerogen samples with Tmax growth at the same time. Moreover mass spectra data of core samples pyrolizates can be collected easily to detect certain groups of compounds. Sulfur-containing compounds temperature extracted by Py-GCMS can be measured for example. Py-GCMS technology fully comply with mainstream analytical protocol for whole-rock or kerogen analysis and even more, Py-GCMS has a lot of advantages against conventional approach providing us additional valuable information about a sample.
МОДЕРНИЗАЦИЯ ЭЛЕКТРОПРИВОДА КОМПРЕССОРНОЙ СТАНЦИИ
Authors: (Lyubov A. Payuk) Паюк Любовь Анатольевна , (Nataliya A. Voronina) Воронина Наталья Алексеевна , (Anatoliy A. Logvinenko) Логвиненко Анатолий Анатольевич
Published: March 2019

Актуальность работы обусловлена необходимостью модернизации различного оборудования на многих старых газокомпрессорных станциях в связи с постройкой газопровода «Сила Сибири». Цель работы: провести анализ пуска электропривода компрессорной установки перекачки природного газа после замены синхронного двигателя на асинхронный. Данная установка является частью большой системы газоснабжения Сибири и Дальнего Востока. Методы: анализ и синтез замкнутых электромеханических систем, математическое описание электропривода переменного тока компрессора в осях x, y, 0 с законом управления U/f2 и учётом особенностей работы на «дожим» природного газа в системе его перекачки. Кроме того, были учтены изменения внутренних параметров исполнительного двигателя (фирмы Siemens типа 1TA2832-4AU01-Z) при увеличении частоты питающего напряжения в пределах от 70 до 150 Гц, а именно уменьшение активных и индуктивных сопротивлений обмоток статора и ротора на 8–12 %, и проведена оценка влияния частоты на динамические показатели электропривода компрессора. Результаты. Получили полную автоматизацию подачи и перекачки природного газа, установили, что изменение частоты питающего напряжения в два раза приводит к увеличению пускового момента на 13 %. Установили, что частота питающего напряжения, равная 136 Гц, является наиболее оптимальной, т. к. позволяет снизить рабочие токи в цепях обмоток статора исполнительного двигателя и устранить проблему бросков токов при пуске, что, в свою очередь, позволило уменьшить потребление электроэнергии в компрессорном цехе на 15–18 %.
РАДИОЭКОЛОГИЧЕСКАЯ ОПАСНОСТЬ ДЛЯ НАСЕЛЕНИЯ В РАЙОНАХ РАСПРОСТРАНЕНИЯ ВЫСОКОРАДИОАКТИВНЫХ ГРАНИТОВ
Authors: (Anastasiya N. Zlobina) Злобина Анастасия Николаевна , (Leonid P. Rikhvanov) Рихванов Леонид Петрович , (Natalia V. Baranovskaya) Барановская Наталья Владимировна , (Iskhak M. Farkhutdinov) Фархутдинов Исхак Мансурович , (Nanping Wang) Ванг Нанпинг
Published: March 2019

Актуальность исследования обусловлена необходимостью объяснения высокой заболеваемости населения в районах распространения радиоактивных пород и почв. Исследование выполнено в рамках нового научного направления – медицинской геологии. Целью работы является изучение масштабов радоновыделений в районах выхода на поверхность высокорадиоактивных гранитов, оценка их радиоэкологической опасности и медико-биологических последствий. Объекты: геохимический и минералогический состав образцов гранитов, кор выветривания и почв, масштабы радоновыделений и частота заболеваемости в различных областях мира (г. Чжухай в китайской провинции Гуандун; г. Эшасьер во французском регионе Овернь) и Сибирского региона (г. Белокуриха Алтайского края; пгт. Колывань Новосибирской области). Методы. В рамках исследования применялся широкий комплекс методов, включающий инструментальный нейтронно-активационный, рентгенофазовый анализы, гамма-спектрометрию, осколочную радиографию, электронную микроскопию, экспрессное измерение объёмной активности радона, расчет потока плотности радона, эквивалентной эффективной дозы и частоты вновь выявленных заболеваний (incidence). Результаты. Проведено обобщение данных по воздействию ионизирующего излучения на организм человека. По результатам геохимических, минералогических, радиоэкологических и медико- статистических исследований выявлено, что в процессах коро- и почвообразования радионуклиды высвобождаются из структурных решеток акцессорных минералов и сорбируются на глинистых минералах. Данный процесс способствует повышению радоновыделений и радиационного фона. Дано объяснение возможных связей повышенных концентраций радионуклидов в подстилающих породах, почвах и высокой активности газа радона с показателями заболеваемости населения. При сопоставлении первичных показателей онкозаболеваемости у всех групп населения с общероссийскими и мировыми показателями отмечаются превышения уровня во всех исследуемых районах. К территориям «риска» по частоте заболеваемости раком легкого можно отнести Белокуриху в Алтайском крае и Колывань в Новосибирской области; раком носоглотки – провинцию Гуандун; раком кроветворной ткани – Колывань; анемиями – Гуандун; по врожденным порокам развития – Гуандун и Колывань.
ГЕОХИМИЧЕСКИЕ ОСОБЕННОСТИ ОРГАНИЧЕСКОГО ВЕЩЕСТВА В ДОННЫХ ОТЛОЖЕНИЯХ ЛАГУНЫ ИВАШКИНОЙ (БЫКОВСКИЙ ПОЛУОСТРОВ, МОРЕ ЛАПТЕВЫХ)
Authors: (Elena V. Gershelis (Panova) Гершелис (Панова) Елена Владимировна , (Ivan V. Goncharov) Гончаров Иван Васильевич , (Oleg V. Dudarev) Дударев Олег Викторович , (Alexey S. Ruban) Рубан Алексей Сергеевич , (Tatyana G. Perevertaylo) Перевертайло Татьяна Геннадьевна , (Kseniya P. Shcherbakova) Щербакова Ксения Павловна , (Natalia E. Shakhova) Шахова Наталья Евгеньевна , (Igor P. Semiletov) Семилетов Игорь Петрович
Published: March 2019

Актуальность. В настоящее время для прогнозирования глобальных климатических изменений особое значение имеет исследование природных механизмов функционирования арктической биогеохимической экосистемы с применением различных методов и аналитических подходов. Органическое вещество современных донных осадков, аккумулирующее гетерогенные сигналы различных процессов транспорта и трансформации углерода, является индикатором уникальных особенностей седиментогенеза и диагенеза осадков. Использование наборов высокоточных геохимических инструментов позволяет получить важную информацию о вкладе аллохтонной и автохтонной компоненты в состав органического вещества и таким образом внести вклад в понимание отдельных элементов современного арктического цикла углерода. Цель исследования заключается в определении литологических и органо-геохимических особенностей отложений, накопленных в специфических лагунных условиях прибрежной части моря Лаптевых (Ивашкина лагуна, Быковский полуостров). Материалы и методы. Объектом исследования был выбран разрез осадков в районе дельты реки Лена. Для оценки изменчивости молекулярного состава органического вещества в процессе накопления толщ осадков был исследован 18-метровый интервал керна скважины VD-13, пробуренной в центральной части Ивашкиной лагуны во время весенней арктической экспедиции 2013 г. Для образцов были определены их гранулометрические характеристики, а также проведены пиролитические и хромато-масс-спектрометрические исследования. Показано, что повышенное содержание органического углерода (Сорг) в разрезе приурочено к пелитовой фракции осадков. Распределение н-алканов характеризуется доминированием высокомолекулярных нечетных гомологов, что указывает на повсеместно определяющий вклад высшей наземной растительности в формирование органического вещества, аккумулируемого в осадках. Тем не менее, вклад потенциально миграционной органической компоненты не исключен, так как для ряда образцов отмечается присутствие низкомолекулярной фракции н-алканов. Результаты пиролитического анализа образцов указывают на резкую изменчивость содержания Сорг и летучих органических соединений с глубиной.
Biochemical investigation of an experimentally induced metabolic syndrome in rats
Authors: Mustafa N.G. , Hasan M.K.
Published: March 2019

Metabolic syndrome (MS) is a complex condition characterized by insulin resistance, hyperglycemia, dyslipidemia, and obesity. This project aims to induce MS in rat and then a demonstration of the main biochemical parameters. In male, Sprague-Dawley rats, MS has been prompted suitably and relatively by fast (six weeks) approach through a high fructose in drinking water (40%). It has been found that serum urea, creatinine, and total bilirubin raise in MS significantly. Moreover, dyslipidemia has arbitrated via some considerable lipid profile deviations. In addition, BMI, blood glucose, and insulin monitoring evidently ensure achievement of MS. It is concluded that a well-established rat model of MS could be employed by a 40% fructose in drinking water.
Effects of the floor type on the gene expression of Hspa1a and cytokines in Holstein dairy cows
Authors: DÝLER Abdulkerim
Published: March 2019

This study was carried out to identify the HSPA1A, TNF, IL1B and IL6 mRNA gene expression levels of Holstein dairy cattle sheltered in different floor types. Nineteen Holstein cows were used in this study. The cattle taken into research were divided into two groups as concrete (CON; n= 10) or rubber mat (RUB; n=9). HSPA1A, TNF, IL1B and IL6 mRNA genes are isolated from milk somatic cells and the gene expression is identified by Real-Time PCR. Between the groups, the HSPA1A (P less than 0.01) and IL1B (P less thann 0.05) gene expression levels were found to be statistically significant, while IL6 and TNF gene expressions were not significant. While the IL6 and TNF gene expression differences are insignificant between the groups, numerically higher level of gene expression was observed in the CON group. Overall results of the study suggested that the rubber mat floor type has a positive impact on both the animal welfare and the udder health.
Multiple-antibiotic resistance of Enterococcus faecalis in an endangered olive ridley sea turtle (Lepidochelys olivacea): A case report
Authors: Tsai Ming-An , Chang Chao-Chin , Li Tsung-Hsien
Published: March 2019

There has recently been an alarming increase in the prevalence of antibiotic resistant bacteria in marine environments. In this study, an Enterococcus sp. was isolated from bacteriologic cultures of the elbow joint of an olive ridley turtle (Lepidochelys olivacea ). The enterococci isolate was identified at the species level as Enterococcus faecalis using PCR and the RAPID ID 32 STREP system. Analysis of the antimicrobial resistance patterns and the antibiotic-resistance genes of the E. faecalis isolated in this study revealed multiple-antibiotic-resistance (MAR). Specifically, the isolate presented resistance to doxycycline, enrofloxacin, erythromycin, neomycin, oxytetracycline, gentamicin, amikacin, ciprofloxacin, spiramycin, chloramphenicol, ceftiofur, and azithromycin. We also identified the tetracycline resistance gene tet(M) in the E. faecalis isolate. Further research into the antimicrobial resistance patterns of Enterococcus spp. should be conducted to promote the conservation of sea turtles.
Use of ordered logit model with time series data for determining the factors affecting the milk yield of Holstein Friesians
Authors: Akkuş Özge , Sevinç Volkan
Published: March 2019

This article aims to introduce the use of ordered logit model with time series data in milk productivity studies and determine the important factor levels affecting the milk yield of Holstein Friesians. The data consists of 2002 records collected for the years 2009-2015 from the reports of the Cattle Breeders’ Association of Turkey (CBAT) in Muðla province in Turkey. The direct and marginal effects of the variables: parity, lactation length and year of calving on milk yield are investigated and the probabilities regarding the milk yield production for a given specific parity, lactation length and calving year are calculated. The results show that milk yield slightly increases on the 4th parity of cows. As far as the years concerned, although there had mostly been a steady amount of milk production between 2009 and 2015 years, there was a significant decrease in 2011 and increase in 2014.
Concanavalin A- Sandwich-ELISA for the detection of antibodies against Newcastle disease virus (NDV) in chicken
Authors: Kannaki T.R. , Priyanka E. , Haunshi Santosh
Published: March 2019

Concanavalin A (Con A), a lectin interacts with carbohydrate moieties of viruses and provide stable and sensitive detection when used as a capture agent. Indirect ELISA methods need purified Newcastle disease virus (NDV) or recombinant antigens for adsorption, whereas use of Con A as capture agent will enable the use of non-purified and non-concentrated virus as antigen replacing costly and time-consuming virus purification step. Con A based sandwich ELISA with non-purified NDV whole virus antigen with single serum dilution format has been developed in this study. The optimum concentrations of the capture agent, Con A and non-purified antigen preparations were determined by checker-board titration. Briefly, microplates were coated with predetermined optimum concentration of ConA (0.5 mg/ml; 50µg per well) and incubated for 18h at 4°C. After washing, allantoic fluid with Newcastle disease virus (NDV) LaSota (HA titre, 210) at a constant predetermined dilution (1:1; 50µl) was coated and incubated for 45 min at 37°C, followed by blocking with 2 % bovine serum albumin for 45 min at 37°C. The antigen coated plates were used in the detection of antibody titre against NDV in serum samples at single serum dilution of 1: 500. Then, wells were added with goat anti-chicken IgG horseradish peroxidase conjugate and incubated for 1h at 37°C, followed by addition of TMB substrate and the plates were read spectrophotometrically at 450 nm. ELISA antibody titres were determined by standard serial dilution of positive sera and endpoints were calculated by a subtraction method. By using positive negative threshold curve (PNT), intercept and slope of the standard curve were calculated. Total of 271 random chicken serum samples were analyzed for antibodies against NDV by Haemagglutination inhibition assay (HI), indirect ELISA and compared with the Con A- S- ELISA developed in this study. The Con A-S-ELISA showed a high coefficient of correlation (r=0.85, n=271, P less than 0.01) and an agreement of ê=0.99 with the commercially available Indirect-ELISA. The relative sensitivity and specificity were 98% and 85% respectively in comparison to HI test. Hence, the Con A-S-ELISA is a simple, easy and effective for monitoring serum antibody levels against NDV.
Disposition Kinetics of lincomycin following intravenous administration in hypothyroid goats
Authors: Sharma Meemansha , Dumka Vinod Kumar , Singla Saloni , Kaur Rajdeep , Singh Raushan Kumar
Published: March 2019

Hypothyroidism is a common disorder of small ruminants and is expected to alter the pharmacokinetics of drugs. Hypothyroidism was induced by feeding thiourea at the dose rate 50 mg.kg-1 daily for 28 days to goats. Disposition of lincomycin, after intravenous administration at dose rate 10 mg/kg, was investigated in hypothyroid goats to determine the potential dosage regimen against susceptible microorganisms. Blood samples were collected from 1 min to 24 h of drug administration. The drug was detected in plasma up to 8 h and lincomycin was rapidly distributed from blood to the tissue, as evidenced by the high value of the distribution coefficient (mean ± SEM) 12.3±1.09 h-1. The large Vd (1.78±0.18 L/kg) indicated vast tissue distribution of lincomycin in goats. The elimination half life, AUC and total body clearance were 3.99± 0.25 h, 33.2±1.71 ìg.h/mL and 0.31±0.02 L/h/kg, respectively. Based on results, lincomycin in hypothyroid goats is suggested to be repeated at 12 h interval for organisms sensitive to lincomycin having MIC up to 0.1 µg.ml-1.
Protective effect of Fragarria ananassa and Vaccinium corymbosum fruit extracts against L-arginine induced acute pancreatitis in rats
Authors: Challa Siva Reddy , Gadicherla Veena , Basaveswara Rao Mandava V. , Ramakrishna P. , Kumar K.Pavan
Published: March 2019

The study was aimed to evaluate the protective effects of alcoholic extracts of Strawberry and Blueberry fruits [AESF and AEBF] in acute pancreatitis in rats. Treatment groups received AESF and AEBF at doses of 200 and 400 mg/kg for 7 days with prior injections of L-arginine on 5th day. Biochemical parameters were estimated in serum and pancreatic tissue samples. Histopathological studies and DNA fragmentation assay were carried out in isolated pancreatic tissue. The results of the study indicated that treatment of AESF and AEBF exhibited a significant dose dependent protective effect. Upon the treatment, anti-oxidant enzymes were significantly (*pless than 0.05) increased. Biochemical results were correlated with the histopathological findings. In addition, the DNA fragmentation assay showed an intact DNA in pancreatic cells of treated groups. In conclusion, berry fruit extracts exerted a potential protective effect against L-arginine induced damage in rat pancreas, at least in part, due to its antioxidant properties.
Antifungal resistance in cnlac1 possessing Cryptococcus neoformans isolated from domestic and feral pigeons in West Bengal, India
Authors: Banerjee A. , Batabyal K. , Debnath C. , Joardar S.N. , Isore D.P. , Dey S. , Taraphder S. , Singh A.D. , Pal S. , Samanta I.
Published: March 2019

Cryptococcus neoformans acts as a major etiology of human infections in immunocompromised, cancer and transplant patients. The present study was conducted to detect the occurrence and antifungal resistance of C. neoformans in domestic and feral pigeons in and around Kolkata, a metropolitan city in India with considerable size of pigeon population. Weathered droppings of domestic and feral pigeons (n=917) were collected from different pet bird shelters, different buildings and other places. Isolation and identification of C. neoformans was performed based on cultural, biochemical properties. Antifungal sensitivity of the confirmed isolates were performed according to standard CLSI protocols with seven antifungals and caspofungin. A total of 153(16.68%) samples were found positive for Cryptococcus neoformans var. neoformans. All the isolates possessed CNLAC1 outer gene in PCR. Antifungal sensitivity revealed marked resistance to amphotericin-B (33.33%), fluconazole (20.91%), flucytosine (11.11%), and ketoconazole (8.49%). This is of great concern as the study area (Kolkata) is a densely populated city and the birds are in close proximity to the people.
Gross morphological and sex wise morphometrical studies on the tenth, eleventh, twelfth and thirteenth thoracic vertebrae of Blue bull (Boselaphus tragocamelus)
Authors: Sathapathy S. , Dhote B.S. , Mahanta D. , Tamilselvan S. , Singh I. , Mrigesh M. , Joshi S.K.
Published: March 2019

The present study was carried out on the tenth, eleventh, twelfth and thirteenth thoracic vertebrae of six specimens of adult Blue bull (Boselaphus tragocamelus) of either sex. The tenth, eleventh, twelfth and thirteenth thoracic vertebrae were characterized by long supraspinous process, cylindrical, but short centrum. The centrum was distinctly constricted in the middle and presented a thin-edged ventral crest. The arch presented shallow notches and was perforated by intervertebral foramina at its caudal aspect. The mammillary processes were fused with the anterior articular processes in T12 and T13. The backward slope of the dorsal supraspinous process decreased from T10 to T12. The supraspinous process of T13 was vertical and wide as lumbar vertebra. The costal facets were placed on either side at the end of the articular extremities of the centrum. However, the posterior costal facets were absent in T13. Each articular facet was a demi-facet which articulated with the half of the part of the head of the rib. The cranial vertebral notches were shallow and small, but the caudal ones were deeper. The arch was caudally perforated by an additional intervertebral foramen on either side. The cranial articular processes were represented by oval facets on the anterior part of the arch and faced upwards except in T11, where they were triangular in shape, whereas the caudal ones sprang from the base of the dorsal supraspinous process. The left caudal articular facets of T13 was placed at a higher level than the right ones.
Effects of oral administration of various essential oils on blood metabolites, intestine development, microbial enumeration and meat quality in broilers
Authors: Liu S.D. , Song M.H. , Yun W. , Lee J.H. , Lee C.H. , Kwak W.G. , Oh H.J. , Kim H.B. , Cho J.H.
Published: March 2019

A total of eighty 21-day-olds (1.1±0.01kg) ROSS 308 broilers were randomly allocated into 4 groups, with 20 replicates per group and 1 chicken per replicate per cage. Three experimental groups (CAR, THY, ORE group) were given oral administration of 200ìL essential oils (carvacrol, thyme and oregano) at 18:00h every day. The results showed no significant differences on blood metabolites among each group (P>0.05). The villus height of the small intestines in the CAR group increased significantly (P less than 0.05) from 1360.10 to 1552.54ìm as compared to the control group, the goblet cell counts in the THY and ORE groups significantly increased by 20.91% (P less than 0.05) and 31.82% (P less than 0.05), respectively, compared to the control group. The E.coli and Salmonella in ORE group significantly decreased by 11.16% (P less than 0.05) and 9.54% (P less than 0.05), respectively, compared to the control group. The Redness (a*) values of the muscles of the CAR and ORE groups were observed to increase significantly (P less than 0.05) from 3.58 to 7.95 and 6.86, respectively, compared to the control group. In conclusion, oral administration of the essential oils carvacrol, thyme, and oregano promoted intestinal development, improved the intestinal environment and the meat quality of broilers.
Effect of strain, sex and rearing system on carcass and fat yield of Naked Neck, Ovambo and Potchefstroom Koekoek chickens
Authors: Mutibvu T. , Chimonyo M. , Halimani T.E.
Published: March 2019

Meat and fat yield influence the profitability of modern commercial poultry production. This study investigated the effect of strain, sex and rearing system on carcass and fat yields of 3 slow-growing chicken strains. Two hundred and eighty-eight Potchefstroom Koekoek, Ovambo and Naked Neck chickens, weighing 1710 ± 308.8 g, were allocated to 4 free-range pens and 4 housed pens. Body (BW), carcass (CW), dressed (DW), cut, giblet, fat pad (AFP) weights were determined after 4 weeks. Analysis of variance (ANOVA) was conducted in SAS. Strain influenced (P less than 0.05) thigh, neck and giblet weight. Males were heavier (P less than 0.05), yielding heavier cuts and pancreases but lighter AFP than females. Ovambos yielded the heaviest portions among the 3 strains. Free-range birds experienced crop, gizzard hypertrophy and pancreatic atrophy. Further research could focus on fatty acid composition of meat from free-range systems to establish potential implications on heart-related health conditions.
Construction and expression of a prokaryotic expression vector for the goat sry gene
Authors: Wang Xiao , E Guang-Xin , Cheng Shu-Zhu , Ni Wei-Wei , Ma Yue-Hui , Chu Ming-Xing , Huang Yong-Fu
Published: March 2019

Goats are economically important animals in the world, and their sex is an important factor in their economic efficiency. Reconstruction of a goat SRY gene expression vector can lay a foundation for studying the immunogenicity and sex determination of SRY protein at the molecular level. In this study, the coding region of the goat SRY gene was used as the target gene fragment for synthesis and optimization, and the cloning vector was used as a template to amplify the target gene and finally ligated to the expression vector pET-SUMO. The recombinant plasmid was then verified by the double restriction enzyme method and transformed into Escherichia coli (DE3). After the induction of expression by Isopropyl â-D-Thiogalactoside (IPTG), the cells were lysed, and SDS-PAGE electrophoresis was performed to observe the expression of the recombinant protein. Additionally, the immunological activity of the recombinant protein was assessed. The target gene was successfully ligated into the prokaryotic expression vector pET-28a; additionally, the prokaryotic expression plasmid pET-SUMO was successfully constructed, and the SRY antigen protein (42 kDa) was expressed. The titer of the rabbit antiserum (PAI-1608012-1) was more than 1:50000, as measured by ELISA, which demonstrated that the titer and the sensitivity of the rabbit serum reached the expected levels. In this study, the prokaryotic expression vector pET-SUMO was successfully constructed. The recombinant protein has high immunopotency and immunoreactivity, which lays a foundation for the preparation of antibodies and the molecular sexing of goats in the future.
Impact of irrigation on pulses production in India: A time-series study
Authors: Vani Gourav Kumar , Mishra Pradeep
Published: March 2019

Pulses are source of protein for Indians, production of which has not kept with increasing demand of the nation. Among efforts to enhance pulses production, role of irrigation as a critical input has not been given due importance. In present investigation attempt is to find out importance and contribution of irrigation in growth of pulses production in India. The time series data on area, production, productivity and area irrigated of pulses was obtained from DES Official website. The regression analysis and linear decomposition analysis were used as tools to carry out analysis. It was found that yield and area not-irrigated effect accounts for 52 per cent of growth of pulses which is not suitable for sustainability of pulses production system. Area not-irrigated effect contribution was 13.69 percent on pulses production. This also shows that irrigation has not been able to influence the production of pulses to desired level. The area irrigated accounted for 69 percent of variation in pulses yield. The result of present investigation is helpful to researcher as well as policymaker in attaining sustainable increases in pulses production in India.
Seed and yield parameters as influenced by season and plant growth regulators in dolichos bean [Lablab purpureus L. (Sweet)]
Authors: Ashok S. Sajjan , . Pramoda , Malabasari T.A. , Shashidhar T.R.
Published: March 2019

Field experiments were conducted in the Department of Seed Science and Technology, University of Agricultural Sciences, Dharwad, Karnataka during 2016-17. The field experiment consisted of 18 treatment combinations involving two seasons viz., kharif 2016 and rabi 2016-17 and plant growth regulators viz., G1: Control, G2 : GA3 @ 50 ppm, G3 : NAA @ 40 ppm, G4 : Cycocel @ 100 ppm, G5 : Mepiquat chloride @ 50 ppm, G6 : Nitrobenzene @ 200 ppm, G7: TIBA @ 50 ppm, G8 : Brassinosteroids @ 200 ppm, G9 : GA3 @ 5 ppm + IAA @ 5 ppm and experiment was laid out in Factorial RBD with three replications. The more number of pods per plant (117.19), number of seeds per pod (4.25), dry pod yield per plant (118.53 g), dry pod yield per plot (2.07 kg), seed yield per plant (114.48 g), pod length (5.66 cm), seed yield per plot (2.32 kg), seed yield per hectare (1,281 kg) was recorded in rabi season than kharif. Among the plant growth regulators more number of pods per plant (120.44), number of seeds per pod (4.44), dry pod yield per plant (120.17 g), dry pod yield per plot (2.21 kg), seed yield per plant (117.60 g), seed yield per plot (2.55 kg), seed yield per hectare (1,360 kg) and pod length (6.04 cm) was recorded in NAA @ 40 ppm, followed by nitrobenzene @ 200 ppm, (119.78, 4.35, 119.54, 2.11, 116.76, 2.42, 1,299 kg and 5.93 cm, respectively). The interaction effect was found to be non-significant for majority of the characters studied. However, foliar spray of NAA @ 40 ppm during rabi season recorded higher crop growth, seed yield and quality parameters as compared to control.
Effect of organic seed pelleting on biometric, biophysical and yield parameters of clusterbean under saline condition
Authors: Prakash M. , Pallavamallan S. , Narayanan G. Sathiya , Rameshkumar S.
Published: March 2019

Cluster bean is one of the major legumes cultivated in many parts of the world. However effect of organic seed pelleting in this crops needs more attention nowadays in probem soils like saline soil. Field experiments were carried out with cluster bean cv. Pusa Mausami to study the effect of organic seed pelleting on biometric, biophysical and yield parameters of clusterbean under saline condition. The seeds of cluster bean cv. Pusa Mausami were pelleted with pungam, prosopis, notchi, arappu leaf powders @ 50, 100, 150 and 200 g per kg. From the results, it was found that seed pelleting with pungam leaf powder @ 200 g per kg registered significantly higher values for biometric parameters viz., plant height, number of branches per plant, dry matter production, biophysical parameters viz., photosynthetic rate, transpiration rate, stomatal conductance, intercellular CO2 concentration and yield parameters viz., pod length, number of pods per plant, pod yield per plant, number of seeds per pod, seed yield per plant and hundred seed weight. From the present study, it was concluded that seed pelleting with pungamn leaf powder @ 200 g per kg can be recommended for increased growth and yield parameters in cluster bean under saline conditions.
Nitrogen use efficiency, economic return and yield performance of Pigeonpea [Cajanus cajan (L.) Millsp.] as influenced by nipping and fertility levels
Authors: Dhaka A.K. , Kumar Satish , Singh Bhagat , Singh Karmal , Kumar Amit , Kumar Navish
Published: March 2019

An experiment was conducted to study nitrogen use efficiency in pigeonpea at Research farm, CCS Haryana Agricultural University, Hisar, India having three nipping treatments (no nipping, nipping at just start of branching and nipping at flower initiation) and five fertility levels (control, 20 kg N + 40 kg P2O5/ha, 30 kg N + 40 kg P2O5 /ha, 40 kg N + 40 kg P2O5/ha and 20 kg N + 40 kg P2O5/ha + foliar spray of 2% N immediately after nipping) replicated thrice in split plot design during growing seasons of 2016 and 2017. Nipping at start of branching reduced the plant height, while increased primary and secondary branches, pods/plant and yield over no nipping. Significantly higher total N uptake, protein content, net return, B: C, agronomical NUE, physiologic NUE, agro-physiologic NUE, apparent recovery efficiency, utilization efficiency of N and partial N balance were improved with nipping at start of branching. Among fertility levels, 40 kg N + 40 kg P2O5 / ha recorded significantly higher yield attributes with 39.7 per cent higher seed yield over control. Significantly higher agronomic NUE, physiologic NUE, agro-physiological NUE, apparent recovery efficiency, utilization efficiency of N, partial N balance and NER were recorded with 20 kg/ha as compared to higher nitrogen doses.
Effect of phosphorus and sulphur application on their dynamics and nodulation in soil under black gram [Vigna mungo (L.) Hepper] crop
Authors: Phogat Mamta , Rai A.P. , Kumar Sunil , Angmo Padma
Published: March 2019

The experiment comprising of four levels of phosphorus, i.e., 0, 20, 40 and 60 kg ha-1 and three levels of sulphur, i.e., 0, 15 and 30 kg ha-1, was conducted during summer of 2015-16 to investigate the effect of phosphorus and sulphur application on their dynamics in soil under the crop of black gram cv. Uttara. The treatments were laid out in randomized block design (factorial) and replicated three times. The results reveal that the available and organic phosphorus (kg ha-1) significantly increased with each successive application of phosphorus in soil up to highest level (60 kg ha-1) at 20 days after sowing (DAS) of black gram, while it showed decreasing trend with time intervals of 40 DAS and at maturity of black gram. The application of successive doses of sulphur had no significant effect on available and organic phosphorus at each time interval. Similarly, significant increase has also been recorded in available and organic sulphur (kg ha-1) in soil with each successive application of sulphur up to 30 kg ha-1 at 20 DAS of black gram, thereafter, it showed decreasing trend. The application of successive doses of phosphorus had no significant effect on available and organic sulphur at each time interval. Number of nodules plant-1 also increased significantly with increasing levels of phosphorus and sulphur up to highest level and the optimum values were recorded with combined application of phosphorus 60 kg ha-1 and sulphur 30 kg ha-1.
Biochemical responses of sainfoin shoot and root tissues to drought stress in In vitro Culture
Authors: Beyaz Ramazan
Published: March 2019

This study was conducted to investigate the biochemical responses of the shoot and root tissues of sainfoin to drought stress under in vitro conditions. Seeds of sainfoin were cultured on MS (Murashige and Skoog) medium with
addition of concentrations of PEG-6000 (50, 100, and 150 g/l). Biochemical analyzes (CAT, SOD, GR, and APX enzyme activity; proline, malondialdehyde (MDA) and chlorophyll contents) were carried out on the 35-day-old seedlings. The principal results
of the study were that CAT and SOD antioxidant enzymes seemed to play a critical role in oxidative stress in both tissues of sainfoin seedlings. On the other hand, a significant decrease in GR activity and no change in APX activity detected in both
tissues under stress. The contents of proline and MDA increased in both tissues while the chlorophyll contents decreased in the shoot tissue. Antioxidant enzyme activities seemed to be more active in the root tissue than the shoot tissue.
Accumulation of proline was higher in the root tissue, while the MDA content was higher in the shoot tissue of the seedlings.
Semi compactness in fuzzifying bitopological spaces
Authors: Allam A. A. , Zahran A. M. , Mousa A. K. , Binshahnah H. M.
Published: March 2019

In this paper, we dene semi open sets in the subspaces of fuzzifying bitopological spaces and study some properties of these sets. We introduce and study the concepts of semi-compactness in fuzzifying bitopological spaces. Also we give some properties of the semi-compactness in fuzzifying bitopological spaces.
Pengaruh Asupan Makanan terhadap Kejadian Malnutrisi di Rumah Sakit
Authors: Dwiyanti Defriani , Hadi Hamam , Susetyowati Susetyowati
Published: March 2019

Background: Sufficient food and other nutrient intake are necessary for recovery of hospitalized patients. Insufficient food intake may decrease nutrient metabolism, which in turn may cause malnutrition. Malnutrition in all hospital is categorized as high (about 40-45%). However, the influence of food intake on hospital malnutrition in Indonesia is still unknown.Objective: To assess the influence of nutrient intake on the occurence of hospital malnutrition.Method: This was an observational with prospective cohort study. This study as conducted in Dr. M. Jamil Padang Hospital, Dr. Sardjito Yogyakarta Hospital, and Sanglah Denpasar Hospital. Subjects were 228 adult patients from internal and neurology units in 1st, 2nd, and 3rd class who were hospitalized for six days or more, receiving oral diet, compos mentis, cooperative, non edema, and non ascites. Data of body weight and body height were measured at admission and discharge. Food intake data collected in this study included hospital and non-hospital food. Data analyzed by FP2 programme and STATA software.Results: Among 228 subjects of study, 51,8% had insufficient intake in the first three days of hospitalization. On average, their protein and energy intakes were lower than subjects with sufficient intake. There was a significant correlation between the first three days intake and intake during hospitalization (p<0,05). Subjects with insufficient energy intake were 2,1 times more likely to be malnourished in hospital than subjects with sufficient energy intake. Meanwhile, subjects with insufficient protein intake were 1,56 times more likely to be malnourished than subjects with sufficient protein intake. There was a significant association between energy intake and hospital malnutrition (p<0,05).Conclusions: Patients with insufficient nutrient intakes are more likely to be malnourished than those with sufficient nutrient intakes.
Faktor risiko obesitas pada ibu rumah tangga miskin
Authors: Purwaningrum Digna Niken , Hadi Hamam , Gunawan I Made Alit
Published: March 2019

Background: Food insecurity is associated with allocation of income for high energy density food consumption that may cause obesity in poor family. In addition, low physical activity may lead to obesity, particularly in individual living in disadvantaged situation.Objective: To identify risk factors of obesity among poor housewives in Yogyakarta.Method: This was a case control study, case group was obese housewives and the control group was non obese housewives. The locations of the study were Bumijo and Pringgokusuman which have high population density. The samples were taken purposively. Each group consisted of 70 housewives (1:1) and were matched according to age. Mc.Nemar test and conditional logistic regression were used to identify the risk factors of obesity.Results: There was no difference in characteristics between the two groups. Food insecurity reached 91,43% in the control group, proportion of excessive energy intake reached 37.86% in the case group, higher than in control group (24.29%). Excessive fat intake in the case group reached 30% whereas in the control group was 28.57%. Low physical activity reached 40% in the case group, and 10% in the control group. The result of Mc.Nemar test showed that food insecurity, energy and fat intake had no significant association with obesity (p>0.05). While physical activity was associated with obesity (p=0.0001). The result of conditional logistic regression showed physical activity was dominant risk factor for obesity among poor housewives (R2=0.1916).Conclusion: Food security status was not a risk factor for obesity in poor families; energy intake and fat intake contributed to the prevalence of obesity though the influence was smaller than physical activity.
Efek ekstrak teh hijau (Camellia sinensis (L.) O. Kuntze var. assamica) terhadap berat badan dan kadar malondialdehid wanita overweight
Authors: Hidayati Alpha Olivia , Lestariana Wiryatun , Huriyati Emy
Published: March 2019

Background: Overweight and obesity were risk factors of various degenerative diseases. Green tea extract contains polyphenol compounds, particularly catechins which act as antioxidants, fat dissolvent and aid lipolysis. Thus, it is necessary to study the use of green tea as an alternative treatment to overcome overweight.Objective: To identify the effect of green tea extract supplementation on body weight and malondialdehide (MDA) level of overweight women in Yogyakarta District Health Office.Method: This was an experimental study with double-blind randomized controlled trial design. Subject of the study were divided into two groups; one group consumed green tea extract capsules and the other consumed placebo. Weight, 6 times 24 hours recall, and level of MDA were measured on every subject.Results: The results revealed that green tea extract has a weight-reducing effect and decrease MDA level of overweight women, though the reduction of weight and MDA level was not significant (p>0,05). Weight loss of 1.3% was observed in the treatment group, whereas the control group has an increase of 0.6% body weight, but the weight change was not significant (p=0.501, p=0.620). Changes in body weight after 12 weeks of treatment showed a significant disparity between the two groups (p=0.000). MDA level decreased by 10% in the treatment group compared to 8.3% in the control group. MDA levels pre and post intervention was significantly different in the treatment group (p=0.006), whereas in the control group there was no significant discrepancy observed (p=0.123). However, changes in MDA levels after 12 weeks of treatment showed no significant differences among groups (p=0.278). The mean nutrient intakes of subjects were still below the recommended dietary allowance (RDA), except for protein.Conclusion: Green tea extract had a weight-reducing effect and decrease MDA level of overweight women in Yogyakarta District Health Office, though the reduction was not statistically significant.