bluuebunny/crossref_metadata_2025 huggingface dataset:
Establishment and Molecular Characterization of Two Patient-Derived Pancreatic Ductal Adenocarcinoma Cell Lines as Preclinical Models for Treatment Response
Authors: Braun Rüdiger , Lapshyna Olha , Watzelt Jessica , Drenckhan Maren , Künstner Axel , Färber Benedikt , Hael Ahmed Ahmed Mohammed , Bolm Louisa , Honselmann Kim Christin , Konukiewitz Björn , Castven Darko , Spielmann Malte , Gorantla Sivahari Prasad , Busch Hauke , Marquardt Jens-Uwe , Keck Tobias , Wellner Ulrich Friedrich , Ungefroren Hendrik
Published: February 2023

The prognosis of pancreatic ductal adenocarcinoma (PDAC) is exceedingly poor. Although surgical resection is the only curative treatment option, multimodal treatment is of the utmost importance, as only about 20% of tumors are primarily resectable at the time of diagnosis. The choice of chemotherapeutic treatment regimens involving gemcitabine and FOLFIRINOX is currently solely based on the patient’s performance status, but, ideally, it should be based on the tumors’ individual biology. We established two novel patient-derived primary cell lines from surgical PDAC specimens. LuPanc-1 and LuPanc-2 were derived from a pT3, pN1, G2 and a pT3, pN2, G3 tumor, respectively, and the clinical follow-up was fully annotated. STR-genotyping revealed a unique profile for both cell lines. The population doubling time of LuPanc-2 was substantially longer than that of LuPanc-1 (84 vs. 44 h). Both cell lines exhibited a typical epithelial morphology and expressed moderate levels of CK7 and E-cadherin. LuPanc-1, but not LuPanc-2, co-expressed E-cadherin and vimentin at the single-cell level, suggesting a mixed epithelial-mesenchymal differentiation. LuPanc-1 had a missense mutation (p.R282W) and LuPanc-2 had a frameshift deletion (p.P89X) in TP53. BRCA2 was nonsense-mutated (p.Q780*) and CREBBP was missense-mutated (p.P279R) in LuPanc-1. CDKN2A was missense-mutated (p.H83Y) in LuPanc-2. Notably, only LuPanc-2 harbored a partial or complete deletion of DPC4. LuPanc-1 cells exhibited high basal and transforming growth factor (TGF)-β1-induced migratory activity in real-time cell migration assays, while LuPanc-2 was refractory. Both LuPanc-1 and LuPanc-2 cells responded to treatment with TGF-β1 with the activation of SMAD2; however, only LuPanc-1 cells were able to induce TGF-β1 target genes, which is consistent with the absence of DPC4 in LuPanc-2 cells. Both cell lines were able to form spheres in a semi-solid medium and in cell viability assays, LuPanc-1 cells were more sensitive than LuPanc-2 cells to treatment with gemcitabine and FOLFIRINOX. In summary, both patient-derived cell lines show distinct molecular phenotypes reflecting their individual tumor biology, with a unique clinical annotation of the respective patients. These preclinical ex vivo models can be further explored for potential new treatment strategies and might help in developing personalized (targeted) therapy regimens.
NbMLP43 Ubiquitination and Proteasomal Degradation via the Light Responsive Factor NbBBX24 to Promote Viral Infection
Authors: Song Liyun , Jiao Yubing , Song Hongping , Shao Yuzun , Zhang Daoshun , Ding Chengying , An Dong , Ge Ming , Li Ying , Shen Lili , Wang Fenglong , Yang Jinguang
Published: February 2023

The ubiquitin–proteasome system (UPS) plays an important role in virus–host interactions. However, the mechanism by which the UPS is involved in innate immunity remains unclear. In this study, we identified a novel major latex protein-like protein 43 (NbMLP43) that conferred resistance to Nicotiana benthamiana against potato virus Y (PVY) infection. PVY infection strongly induced NbMLP43 transcription but decreased NbMLP43 at the protein level. We verified that B-box zinc finger protein 24 (NbBBX24) interacted directly with NbMLP43 and that NbBBX24, a light responsive factor, acted as an essential intermediate component targeting NbMLP43 for its ubiquitination and degradation via the UPS. PVY, tobacco mosaic virus, (TMV) and cucumber mosaic virus (CMV) infections could promote NbMLP43 ubiquitination and proteasomal degradation to enhance viral infection. Ubiquitination occurred at lysine 38 (K38) within NbMLP43, and non-ubiquitinated NbMLP43(K38R) conferred stronger resistance to RNA viruses. Overall, our results indicate that the novel NbMLP43 protein is a target of the UPS in the competition between defense and viral anti-defense and enriches existing theoretical studies on the use of UPS by viruses to promote infection.
Genotoxic Risks to Male Reproductive Health from Radiofrequency Radiation
Authors: Kaur Puneet , Rai Umesh , Singh Rajeev
Published: February 2023

During modern era, mobile phones, televisions, microwaves, radio, and wireless devices, etc., have become an integral part of our daily lifestyle. All these technologies employ radiofrequency (RF) waves and everyone is exposed to them, since they are widespread in the environment. The increasing risk of male infertility is a growing concern to the human population. Excessive and long-term exposure to non-ionizing radiation may cause genetic health effects on the male reproductive system which could be a primitive factor to induce cancer risk. With respect to the concerned aspect, many possible RFR induced genotoxic studies have been reported; however, reports are very contradictory and showed the possible effect on humans and animals. Thus, the present review is focusing on the genomic impact of the radiofrequency electromagnetic field (RF-EMF) underlying the male infertility issue. In this review, both in vitro and in vivo studies have been incorporated explaining the role of RFR on the male reproductive system. It includes RFR induced-DNA damage, micronuclei formation, chromosomal aberrations, SCE generation, etc. In addition, attention has also been paid to the ROS generation after radiofrequency radiation exposure showing a rise in oxidative stress, base adduct formation, sperm head DNA damage, or cross-linking problems between DNA & protein.
The Role of CD38 in the Pathogenesis of Cardiorenal Metabolic Disease and Aging, an Approach from Basic Research
Authors: Kitada Munehiro , Araki Shin-ichi , Koya Daisuke
Published: February 2023

Aging is a major risk factor for the leading causes of mortality, and the incidence of age-related diseases including cardiovascular disease, kidney disease and metabolic disease increases with age. NAD+ is a classic coenzyme that exists in all species, and that plays a crucial role in oxidation–reduction reactions. It is also involved in the regulation of many cellular functions including inflammation, oxidative stress and differentiation. NAD+ declines with aging in various organs, and the reduction in NAD+ is possibly involved in the development of age-related cellular dysfunction in cardiorenal metabolic organs through the accumulation of inflammation and oxidative stress. Levels of NAD+ are regulated by the balance between its synthesis and degradation. CD38 is the main NAD+-degrading enzyme, and CD38 is activated in response to inflammation with aging, which is associated with the reduction in NAD+ levels. In this review, focusing on CD38, we discuss the role of CD38 in aging and the pathogenesis of age-related diseases, including cardiorenal metabolic disease.
In Silico Identification and Functional Characterization of Genetic Variations across DLBCL Cell Lines
Authors: Dharanipragada Prashanthi , Parekh Nita
Published: February 2023

Diffuse large B-cell lymphoma (DLBCL) is the most common form of non-Hodgkin lymphoma and frequently develops through the accumulation of several genetic variations. With the advancement in high-throughput techniques, in addition to mutations and copy number variations, structural variations have gained importance for their role in genome instability leading to tumorigenesis. In this study, in order to understand the genetics of DLBCL pathogenesis, we carried out a whole-genome mutation profile analysis of eleven human cell lines from germinal-center B-cell-like (GCB-7) and activated B-cell-like (ABC-4) subtypes of DLBCL. Analysis of genetic variations including small sequence variants and large structural variations across the cell lines revealed distinct variation profiles indicating the heterogeneous nature of DLBCL and the need for novel patient stratification methods to design potential intervention strategies. Validation and prognostic significance of the variants was assessed using annotations provided for DLBCL samples in cBioPortal for Cancer Genomics. Combining genetic variations revealed new subgroups between the subtypes and associated enriched pathways, viz., PI3K-AKT signaling, cell cycle, TGF-beta signaling, and WNT signaling. Mutation landscape analysis also revealed drug–variant associations and possible effectiveness of known and novel DLBCL treatments. From the whole-genome-based mutation analysis, our findings suggest putative molecular genetics of DLBCL lymphomagenesis and potential genomics-driven precision treatments.
Surgical Models of Liver Regeneration in Pigs: A Practical Review of the Literature for Researchers
Authors: Cinelli Lorenzo , Muttillo Edoardo Maria , Felli Emanuele , Baiocchini Andrea , Giannone Fabio , Marescaux Jacques , Mutter Didier , De Mathelin Michel , Gioux Sylvain , Felli Eric , Diana Michele
Published: February 2023

The remarkable capacity of regeneration of the liver is well known, although the involved mechanisms are far from being understood. Furthermore, limits concerning the residual functional mass of the liver remain critical in both fields of hepatic resection and transplantation. The aim of the present study was to review the surgical experiments regarding liver regeneration in pigs to promote experimental methodological standardization. The Pubmed, Medline, Scopus, and Cochrane Library databases were searched. Studies evaluating liver regeneration through surgical experiments performed on pigs were included. A total of 139 titles were screened, and 41 articles were included in the study, with 689 pigs in total. A total of 29 studies (71% of all) had a survival design, with an average study duration of 13 days. Overall, 36 studies (88%) considered partial hepatectomy, of which four were an associating liver partition and portal vein ligation for staged hepatectomy (ALPPS). Remnant liver volume ranged from 10% to 60%. Only 2 studies considered a hepatotoxic pre-treatment, while 25 studies evaluated additional liver procedures, such as stem cell application, ischemia/reperfusion injury, portal vein modulation, liver scaffold application, bio-artificial, and pharmacological liver treatment. Only nine authors analysed how cytokines and growth factors changed in response to liver resection. The most used imaging system to evaluate liver volume was CT-scan volumetry, even if performed only by nine authors. The pig represents one of the best animal models for the study of liver regeneration. However, it remains a mostly unexplored field due to the lack of experiments reproducing the chronic pathological aspects of the liver and the heterogeneity of existing studies.
Hodgkin Lymphoma Cell Lines and Tissues Express mGluR5: A Potential Link to Ophelia Syndrome and Paraneoplastic Neurological Disease
Authors: Schnell Sofia , Knierim Ellen , Bittigau Petra , Kreye Jakob , Hauptmann Kathrin , Hundsdoerfer Patrick , Morales-Gonzalez Susanne , Schuelke Markus , Nikolaus Marc
Published: February 2023

Ophelia syndrome is characterized by the coincidence of severe neuropsychiatric symptoms, classical Hodgkin lymphoma, and the presence of antibodies to the metabotropic glutamate 5 receptor (mGluR5). Little is known about the pathogenetic link between these symptoms and the role that anti-mGluR5-antibodies play. We investigated lymphoma tissue from patients with Ophelia syndrome and with isolated classical Hodgkin lymphoma by quantitative immunocytochemistry for mGluR5-expression. Further, we studied the L-1236, L-428, L-540, SUP-HD1, KM-H2, and HDLM-2 classical Hodgkin lymphoma cell lines by FACS and Western blot for mGluR5-expression, and by transcriptome analysis. mGluR5 surface expression differed significantly in terms of receptor density, distribution pattern, and percentage of positive cells. The highest expression levels were found in the L-1236 line. RNA-sequencing revealed more than 800 genes that were higher expressed in the L-1236 line in comparison to the other classical Hodgkin lymphoma cell lines. High mGluR5-expression was associated with upregulation of PI3K/AKT and MAPK pathways and of downstream targets (e.g., EGR1) known to be involved in classical Hodgkin lymphoma progression. Finally, mGluR5 expression was increased in the classical Hodgkin lymphoma-tissue of our Ophelia syndrome patient in contrast to five classical Hodgkin lymphoma-patients without autoimmune encephalitis. Given the association of encephalitis and classical Hodgkin lymphoma in Ophelia syndrome, it is possible that mGluR5-expression in classical Hodgkin lymphoma cells not only drives tumor progression but also triggers anti-mGluR5 encephalitis even before classical Hodgkin lymphoma becomes manifest.
Intergenerational Deliberations for Long Term Sustainability
Authors: Spencer Llinos Haf , Lynch Mary , Thomas Gwenlli Mair , Edwards Rhiannon Tudor
Published: February 2023

Grŵp Cynefin, a social housing association in North Wales, United Kingdom (UK) with other partner organisations, had a vision to create a community Hub in the Nantlle Valley to strengthen and support the health and well-being of the local community through the provision of a range of traditional and preventative services. Social prescribing (SP), which is a non-medical support using community assets, would be a part of this new innovative Hub. SP activities would be co-designed and co-produced by current community members. Drawing on the principles of citizens’ assembly deliberations and Future Design, four focus groups (n = 16) were conducted to develop sustainable strategies for SP activities as part of the proposed Hub. Deliberations on the perspectives of future generations were considered along with current community needs. Findings from the focus groups imply that current members of society are open to the concept of taking an inter-generational approach when designing SP activities to address the social and economic needs of the community along with integration of traditional and preventative community health services. Deliberations highlighted that the proposed Hub could strengthen communities and support community health and well-being, by providing a place to socialise and acting as a single point of access for community services, which could promote social cohesion in line with the Well-being for Future Generations (Wales) Act. Applying a long-term thinking approach to citizens’ assembly deliberation design offers a voice to the interests of future generations, providing inter-generational equity.
Formaldehyde Gas Sensors Fabricated with Polymer-Based Materials: A Review
Authors: Min Yuru , Yuan Chenyao , Fu Donglei , Liu Jingquan
Published: February 2023

Formaldehyde has been regarded as a common indoor pollutant and does great harm to human health, which has caused the relevant departments to pay attention to its accurate detection. At present, spectrophotometry, gas chromatography, liquid chromatography, and other methods have been proposed for formaldehyde detection. Among them, the gas sensor is especially suitable for common gaseous formaldehyde detection with the fastest response speed and the highest sensitivity. Compared with the formaldehyde sensors based on small molecules, the polymer-based sensor has higher selectivity but lower sensitivity because the polymer-based sensor can realize the specific detection of formaldehyde through a specific chemical reaction. Polymer-related formaldehyde sensors can be very versatile. They can be fabricated with a single polymer, molecularly imprinted polymers (MIP), polymer/metal-oxide composites, different polymers, polymer/biomass material composites, polymer/carbon material composites, and polymer composites with other materials. Almost all of these sensors can detect formaldehyde at ppb levels under laboratory conditions. Moreover, almost all polymer nanocomposite sensors have better sensitivity than single polymer sensors. However, the sensing performance of the sensor will be greatly reduced in a humid environment due to the sensitive coating on the gaseous formaldehyde sensor, which is mostly a hydrophilic polymer. At present, researchers are trying to improve the sensitive material or use humidity compensation methods to optimize the gaseous formaldehyde sensor. The improvement of the practical performance of formaldehyde sensors has great significance for improving indoor living environments.
Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay Method
Authors: Mordvinova Ekaterina D. , Nikitina Polina A. , Yarovaya Olga I. , Volosnikova Ekaterina A. , Murashkin Denis E. , Isaeva Anastasiya A. , Koldaeva Tatiana Y. , Perevalov Valery P. , Salakhutdinov Nariman F. , Shcherbakov Dmitriy N.
Published: February 2023

Angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV-2, plays a crucial role in the pathogenesis of COVID-19. ACE2 targeting holds the promise for preventing and inhibiting SARS-CoV-2 infection. In this work, we describe the development and use of a test system based on competitive ELISA for the primary screening of potential antiviral compounds. We studied the activity of the library of dyes of different groups. Several dyes (ortho-cresolphthalein, eosin (free acid), eosin (Na salt)) that inhibited the interaction of ACE2 with the spike proteins of SARS-CoV-2 have been identified among the candidates. A potential antiviral drug, methylene blue, did not show activity in our study. We believe that our results can help in the further search for inhibitors of interaction between the coronavirus spike protein and ACE2 receptor.
Dental Phenotype with Minor Ectodermal Symptoms Suggestive of WNT10A Deficiency
Authors: García-Martínez Victoria-Eugenia , Galiana-Vallés Ximo , Zomeño-Alcalá Otilia , Rodríguez-López Raquel , Llena Carmen , Martínez-Romero María del Carmen , Guillén-Navarro Encarna
Published: February 2023

Ectodermal dysplasias (EDs) represent a heterogeneous group of genetic disorders characterized by the abnormal development of ectodermal-derived tissues. They include the involvement of the hair, nails, skin, sweat glands, and teeth. Pathogenic variants in EDA1 (Xq12–13.1; OMIM*300451), EDAR (2q11-q13; OMIM*604095), EDARADD (1q42-q43, OMIM*606603), and WNT10A (2q35; OMIM*606268) genes are responsible for most EDs. Bi-allelic pathogenic variants of WNT10A have been associated with autosomal recessive forms of ED, as well as non-syndromic tooth agenesis (NSTA). The potential phenotypic impact of associated modifier mutations in other ectodysplasin pathway genes has also been pointed out. We present on an 11-year-old Chinese boy with oligodontia, with conical-shaped teeth as the main phenotype, and other very mild ED signs. The genetic study identified the pathogenic variants WNT10A (NM_025216.3): c.310C > T; p. (Arg104Cys) and c.742C > T; p. (Arg248Ter) in compound heterozygosis, confirmed by parental segregation. In addition, the patient had the polymorphism EDAR (NM_022336.4): c.1109T > C, p. (Val370Ala) in homozygosis, named EDAR370. A prominent dental phenotype with minor ectodermal symptoms is very suggestive of WNT10A mutations. In this case, the EDAR370A allele might also attenuate the severity of other ED signs.
Analysis of Cause-of-Death Mortality in Children and Young Adults with Diabetes: A Nationwide 10-Year Follow-Up Cohort Study
Authors: Choi Iee-Ho , Yeom Sang-Woo , Kim Sun-Young , You Jihye , Kim Jong-Seung , Kim Minsun
Published: February 2023

We examined the associations of clinical characteristics and cause-of-death patterns with mortality in children and young adults (<30 years) with diabetes. We analyzed a nationwide cohort sample from the KNHIS database using propensity score matching from a sample of 1 million people from 2002 to 2013. There were 10,006 individuals in the diabetes mellitus (DM) group and 10,006 in the control (no DM) group. The numbers of deaths were 77 in the DM group and 20 in the control group. The deaths of patients in the DM Group were 3.74 (95% confidence interval (CI) = 2.25–6.21) times higher than in the control group. Type 1 DM, type 2 DM and unspecified DM were 4.52 (95% CI = 1.89–10.82) times, 3.25 (95% CI = 1.95–5.43) times and 10.20 (95% CI = 5.24–20.18) times higher, respectively. Mental disorders were 2.08 times higher in the risk of death (95% CI = 1.27–3.40). Mortality rates have increased in children and young adults with diabetes alone. Therefore, in the future, it is necessary to identify the cause of the increased mortality rate among young diabetic people and select vulnerable groups among them so that early prevention can be achieved.
Monocrystalline Nickel Nanogrinding Subsurface Deformation-Layer Depth Study Based on Orthogonal Tests
Authors: Ren Jie , Lv Ming
Published: February 2023

Nanogrinding is one of the main technologies for machining complex surface shapes with nanometer-level precision. The subsurface deformation depth, as an important index of machining quality, directly affects the service life and mechanical properties of machined parts. In order to explore the factors that influence subsurface deformation depth, this work investigated the effects of three factors, namely, grinding speed, grinding depth and crystal orientation, along different crystal planes at the depth of the subsurface deformation layer in a monocrystalline nickel nanofabrication process. By combining molecular dynamics simulation and orthogonal tests, the results showed that, among the three aforementioned factors, the influence of crystal orientation at the depth of the subsurface deformation layer was the greatest, followed by that of grinding depth, while the influence of grinding speed was the weakest. Through the orthogonal tests, the factors affecting the significance of subsurface deformation depth were analyzed, and the results were found to be more meaningful compared with those of current single-factor studies. Meanwhile, in-depth exploration of the nanogrinding mechanism can provide the necessary theoretical basis for the development of nanomachining technology, which is of great significance for the improvement of ultra-precision cutting technology.
Bibliometric Evidence on the Trend and Future Direction of the Research on Textile Coloration with Natural Sources
Authors: Mamun Md. Abdullah Al , Haji Aminoddin , Mahmud Md. Himel , Repon Md. Reazuddin , Islam Mohammad Tajul
Published: February 2023

Synthetic dyes used for the coloration of textile goods are not readily biodegradable and are a major concern for water pollution. Nature has abundant sources of potential colorants for textile applications. There are many challenges in textile coloration using natural dyes and pigments, and significant research efforts are currently put into replacing synthetic textile dyes successfully. In order to gain insight into the future trajectory of dyeing research utilizing natural colorants, a bibliometric analysis from 1990 to 2021 using the Science Citation Index Expanded database was conducted. The analysis focused on how well the publication performed in terms of outputs and citations annually, mainstream journals, Web of Science categories, top universities, top nations, research trends, and hotspots. An overview of the most frequently used keywords derived from terms in the article title analysis, authors’ keyword analysis, and KeyWords Plus analysis served as the foundation for determining current research goals and future trends. The findings indicated that no noteworthy research on this topic was conducted in the final decade of the previous century but that it did begin to get the attention of scholars in the first decade of this century. The use of natural dyes in industry has significantly expanded during the past ten years. “Fastness” is the interest point that has received the most attention. Mordants, or environmentally friendly extraction techniques such as ultrasonic, gamma irradiation, etc., represent the future of this research area. On the other hand, ultraviolet protection and antibacterial or antimicrobial properties are becoming more and more popular in the field of textile dyeing research using natural colorants.
Pullulan Films with PCMs: Recyclable Bio-Based Films with Thermal Management Functionality
Authors: Kizildag Nuray
Published: February 2023

The use of phase-changing materials (PCMs) is a practical and powerful way of buffering thermal fluctuations and maintaining the isothermal nature of the storage process. In this study, melamine formaldehyde microcapsules with paraffin cores were used as PCMs; pullulan films with PCMs were prepared by the film-casting method; and the composite films prepared were analysed with regard to their chemical structure, thermal properties, thermal stability, and recyclability. Uniform films displaying thermal management functionality were prepared. The amount of 75 wt.% PCM were added to the pullulan film structure which enabled the preparation of a composite film that displayed 104.85 J g−1 of heat storage during heating and 103.58 J g−1 of heat release during cooling. Multiple heating and cooling cycles showed that the composite films maintained their thermal management functionality after multiple heating-cooling cycles. The PCMs could be recovered with a yield of approximately 95% by the application of a simple dissolution and filtration process. The morphology, chemical structure, and thermal properties of the PCMs were maintained after the recovery process. The bio-based composite films with thermal management functionality and recyclability are proposed as an innovative, practical, and effective system for thermoactive storage and packaging applications.
Measurement of Tire-Pavement Contact Tri-Axial Stress Distribution Based on Sensor Array
Authors: Guan Jiaxi , Zhou Xinglin , Liu Lu , Ran Maoping
Published: February 2023

A tire’s three-dimensional stress for pavement is an important cause of asphalt pavement disease. In order to study the contact stress distribution between the tire and the pavement under real conditions, a sensor that can measure the tri-axial stress synchronously is designed, and a complete measurement system is established. The variation trend and stress value of tri-axial stress under steady rolling of the tire were obtained, and the stress distribution characteristics were analyzed. The results show that the stress in the three directions near the tire shoulder is greater than that in the crown area, and the stress peak moves gradually from front to back with the rolling of the tire. Compared with the simplified simulation model, these results provides valuable suggestions for exploring the real tire-pavement interaction.
Effect of Ni Coating on Microstructure and Property of Al Alloy/Steel CMT Welding-Brazing Joints
Authors: Zhang Chao , Wu Mingfang , Pu Juan , Rao Jiawei , Long Weimin , Shen Yuanxun
Published: February 2023

The cold metal transfer (CMT) welding-brazing process was chosen to join Al alloy and Ni-coated steel using AlSi12 as the filler wire. The macrostructure and microstructure of the joints were tested by using an optical microscope (OM), scanning electron microscope (SEM), energy dispersive spectrometry (EDS), and X-ray diffraction (XRD). The tensile properties and corrosion properties of the joints were also tested. The results showed that Ni coating could improve the wettability and spreadability of molten AlSi12 filler metal on the steel surface, resulting in a good appearance for the Al alloy/steel joint. Ni coating could hinder the chemical metallurgical reaction between Al atom and Fe atoms to inhibit the formation of brittle Fe-Al intermetallic compounds (IMCs) and reduce the thickness of the IMCs layer. Meanwhile, the Ni atom reacted with the Fe and Al atoms to form Al3Ni2, (Fe, Ni) Al3 and (Fe, Ni)2Al3, which improved the tensile strength of the joints. All joints with Ni coating cracked near the Al alloy. When the Ni-coating thickness was 5 μm, the tensile strength of the joint reached a maximum of 202.5 MPa. The addition of Ni could also improve the corrosion resistance of the joints. Significantly, when the Ni-coating thickness was 10 μm, most of the Ni coating was still solid, and the interface reaction layer was mainly composed of α-Ni solid solution and some (Fe, Ni)2Al3.
Impact of Zinc Oxide Nano Particles, Poly Vinyl Alcohol, and Natural Polymers on Quality Characteristics of Nanocomposite Film
Authors: Singh Gurpreet , Shanmugam Sivakumar , Chawla Rekha , Goel Nitika , Talwar Gopika , Mishra Santosh Kumar , Chatli Manish Kumar
Published: February 2023

The use of biodegradable films to replace the synthetic polymers prepared from natural polymers has been strongly limited owing to their poor barrier and mechanical properties. The modification was carried out with a partial replacement of natural polymers with synthetic polymer, such as PVA (poly vinyl alcohol), to increase the barrier properties of the film. The addition of an active ingredient in the form of nanoparticles such as Zinc Oxide (ZnO), enhanced the properties of the packaging materials compared to the conventional composite film, to which sonication imparted an excellent dispersion of nanoparticles in the slurry. The film thickness, water vapor permeability, film solubility, and mechanical properties of the composite, the composite with PVA, and the composite with PVA and ZnO nanoparticle film (active film) values differed significantly (p < 0.05) between the samples. The Z-average diameters of the composite slurry, the composite with PVA slurry, and the composite with PVA and ZnO nanoparticle slurry ranged from 242.20 to 1021.03 in nanometers (d-nm). The PDI and zeta potential of the samples were also analyzed. The antibacterial activity of the nanoparticles showed a distinct inhibition against Gram-positive Bacillus cereus and Gram-negative Escherichia coli in the treated films counterpart to the control films. The active film conferred excellent mechanical and barrier properties, including antibacterial properties.
Hard Carbons Derived from Phenyl Hyper-Crosslinked Polymers for Lithium-Ion Batteries
Authors: Guo Ziyang , Tian Xiaodong , Song Yan , Yang Tao , Ma Zihui , Gong Xiangjie , Wang Chao
Published: February 2023

Hyper-crosslinked polymers are attracting extensive attention owing to their ease of design and synthesis. Based on the flexibility of its molecular design, a hyper-crosslinked polymer with a π-conjugated structure and its derived carbon were synthesized by the Friedel–Crafts reaction. The polymer and its derived hard carbon material were characterized by FTIR, 13C NMR, Raman, BET, and other characterization tools. The electrochemical properties of both materials as anode electrodes of lithium-ion batteries were investigated. Benefiting from the highly cross-linked skeleton and conjugated structure, the as-prepared carbon materials still had high specific surface area (583 m2 g−1) and porosity (0.378 cm3 g−1) values. The hard carbon (CHCPB) anode possessed the powerful reversible capacity of 699 mAh g−1 at 0.1A g−1, and it had an excellent rate of performance of 165 mAh g−1 at the large current density of 5.0 A g−1. Long-cycle performance for 2000 charge/discharge cycles displayed that the capacity was kept at 148 mAh g−1 under 2 A g−1. This work contributes to a better understanding of the properties of hard carbon materials derived from hyper-crosslinked polymers and how this class of materials can be further exploited in various applications.
An Analytical Surface Potential and Effective Charge Density Approach Based Drain Current Model for Amorphous InGaZnO Thin-Film Transistors
Authors: Song Zhaoxu , Wang Shichun , Han Yujie , Huang Gongyi , Xu Chuanzhong
Published: February 2023

An analytical surface-potential-based drain current model for amorphous indium–gallium–zinc–oxide (a-InGaZnO) thin film transistors (TFTs) is proposed by introducing an effective charge density approach in this paper. This approach gives two initial approximate values of the effective state density and the effective thermal voltage by using the dominant state of the free charge density in total charge density, and then obtains a high-precision one-exponent equivalent transformation for three-exponent total charge density. Based on this approach, we have solved the problem that the physical meaning of the transition area in the regional method is not clear and a one-piece analytical surface potential solution to Poisson’s equation is successfully derived. Furthermore, the drain current is also explicitly derived from the charge sheet model and I-V characteristics of a-InGaZnO TFTs are reproduced from the above obtained model. Finally, accurate and effective surface-potential model and drain current model are obtained and verified by experimental data, respectively. Good verification results prove that the proposed model could become an accurate and suitable tool for being embedded into a circuit simulation.
Biostable Fluorine-Containing Coatings on the Surface of Polymers
Authors: Doronin Fedor , Rytikov Georgy , Evdokimov Andrey , Rudyak Yury , Savel’ev Mikhail , Nazarov Victor
Published: February 2023

We considered the formation of the integrated biostable coating on the surface of several polymers using the original gas-phase fluorination technology. The effectiveness of surface modification of low-density polyethylene (LDPE) and polypropylene (PP) was confirmed by corresponding changes in the absorption spectra of infrared radiation with the modified samples. The gas-phase fluorination quality of ultrahigh molecular weight polyethylene (UHMWPE) and nitrile butadiene rubber (NBR) surfaces was evidenced by the results of scanning electron microscopy (SEM) and energy dispersion analysis (EDS). As was shown with the direct measurements of water and ethyleneglycol wetting angles, the properties differences of the polymer materials with modified surfaces from the initial one correlated well with the change in free surface energy induced by the chemical and morphological transformations under the fluorine and oxygen-containing gas mixtures treatment. The developed technique for the bio-contamination degree quantifying, based on the computer analysis of the corresponding images, allowed us to establish that gas-phase fluorination of the surface approximately doubles the time during which the biofouling of LDPE, PP and UHMWPE with the mixed colony of Rivularia and Stigonema Minutum algae occurs under natural conditions. No positive effect from fluorination was observed for NBR, which was explained, from our point of view, by the difference in the mechanisms of biostability for the considered polymer materials.
Solvent Effect on Small-Molecule Thin Film Formation Deposited Using the Doctor Blade Technique
Authors: Ramos-Hernández Rodrigo , Pérez-Gutiérrez Enrique , Calvo Francisco Domingo , Beristain Miriam Fatima , Cerón Margarita , Percino Maria Judith
Published: February 2023

Small molecule thin films are the core of some organic optoelectronic devices. Their deposition by solution processes is an advantage for device fabrication and can be achieved via spin coating for small areas and slot-die or doctor blade for larger areas. Solution deposition of small molecule thin films is usually processed only with medium polarity solvents. Herein, the use and influence of solvents with several polarities and physicochemical properties to form small-molecule homogeneous thin films via the doctor blade technique over an area of 25 cm2 have been explored. Solvents with different polarity, heptane, chlorobenzene, N,N-dimethylformamide, acetonitrile, and methanol were used along with different deposition temperatures, from room temperature up to near the boiling temperature for each solvent. With heptane and chlorobenzene, smooth films with an average roughness of 3 nm and thickness of 100–120 nm were obtained. The film was homogeneous over the whole substrate for temperatures from room temperature to close to the boiling temperature of both solvents. On the other hand, with dimethylformamide, a film is observed when the deposition is conducted only at room temperature; when the deposition temperature increases, the formation of agglomerates of several sizes from 1 to 5 nm was observed. With acetonitrile, and methanol, no films were formed, and only nanoaggregates were created on the substrate due to the solvent high vapor pressure, and the agglomerate size depends on the deposition temperature. The measure of the contact angle of pure solvent and solutions indicated that wettability helps to film formation over the whole substrate. For heptane and chlorobenzene, a small angle was measured; meanwhile, the contact angle is large in acetonitrile leading to the formation of nanoaggregates. In the case of methanol solution, although it wets very well, no film is deposited because it has high volatility.
Evaluation of the Minority-Carrier Lifetime of IMM3J Solar Cells under Proton Irradiation Based on Electroluminescence
Authors: Xu Jing , Yan Gang , Lu Ming
Published: February 2023

The shortening of the minority carrier lifetime is the main reason for the degradation of the electrical performance of solar cells; therefore, it is particularly important to evaluate the minority carrier lifetime of inverted metamorphic triple junction (IMM3J) GaInP/GaAs/InGaAs solar cells. We evaluate the minority carrier lifetime of each subcell of IMM3J solar cells before and after 2 MeV proton irradiation by the electroluminescence (EL) method. Before proton irradiation, the minority carrier lifetimes of the GaInP, GaAs, and InGaAs subcells were 6.99 × 10−9 s, 3.09 × 10−8 s, and 2.31 × 10−8 s, respectively. After proton irradiation, the minority carrier lifetime of GaInP, GaAs, and InGaAs subcells degraded significantly. When the proton fluence was 2 × 1012 cm−2, the minority carrier lifetimes of the GaInP, GaAs, and InGaAs subcells degraded to 1.63 × 10−10 s, 1.56 × 10−11 s, and 1.65 × 10−10 s, respectively. These results provide a reference for predicting the degradation of the short-circuit current and open-circuit voltage of each subcell.
Study on Oscillatory Mechanism of Solutocapillary Convection and Influence of Aspect Ratio on Flow Characteristics during Crystal Growth
Authors: Zhang Shuo , Liang Ruquan , Yang Shuo
Published: February 2023

A numerical simulation has been conducted to investigate the oscillatory mechanism of a solutocapillary convection and the influence of different aspect ratios on the flow characteristics in a liquid bridge. The SIMPLE algorithm is applied to figure out the Navier-Stokes equation and the concentration diffusion equation on the staggered grids, and the level set approach with the conservation of the mass is used to acquire the surface deformation of the liquid bridge. The flow characteristics of the oscillatory solutocapillary convection are analyzed in detail, including the distributions of the concentration, velocity, and transverse displacement of the free surface at the upper corner and intermediate height of the liquid bridge. Moreover, the effects of the aspect ratio on the flow stability and onset time of the oscillations for the concentration and velocity have also been investigated. The results show that the essence of the oscillatory solutocapillary convection is the result of the coupling oscillation of the concentration, velocity and free surface. The upper corner is the origin region of the oscillation, which has an important impact on the overall flow characteristics. Within a definite height range of the liquid bridge, the lower the height, the more stable the flow and the weaker the oscillation. There is a complex relationship between the onset time of the concentration and velocity oscillations and the aspect ratio of the liquid bridge.
Influence of Rapid Thermal Annealing on the Characteristics of Sn-Doped Ga2O3 Films Fabricated Using Plasma-Enhanced Atomic Layer Deposition
Authors: Shen Yi , Ma Hong-Ping , Wang Zhen-Yu , Gu Lin , Zhang Jie , Li Ao , Yang Ming-Yang , Zhang Qing-Chun
Published: February 2023

In this work, Sn-doped Ga2O3 films fabricated using plasma-enhanced atomic layer deposition were treated by rapid thermal annealing (RTA). The RTA influence on the chemical state, surface morphology, energy band alignment, and electrical properties of Sn-doped Ga2O3 films were thoroughly investigated. The results of X-ray photoelectron spectroscopy (XPS) demonstrated that Sn atoms were successfully doped into these films. Moreover, energy band alignments were obtained by the energy-loss peak of the O 1s spectrum and valence band spectra and thoroughly discussed. X-ray reflectivity (XRR) and atomic force microscope (AFM) measurements indicated that the Sn-doping level affects the interfacial microstructure and surface morphology. As the Sn content increases, the film thickness decreases while the roughness increases. Finally, the leakage current-voltage (I-V) characteristics proved that the Sn-doped Ga2O3 films have a large breakdown field. In I-V tests, all metal oxide semiconductor (MOS) capacitors exhibited a hard breakdown. This research demonstrates a method for manufacturing high-performance optoelectronic devices with desired properties.
Temperature Sensing Based on Defect Mode of One-Dimensional Superconductor-Semiconductor Photonic Crystals
Authors: Wei Huisheng , Chen Xiaoling , Zhao Dong , Zhao Miaomiao , Wang Yang , Zhang Pu
Published: February 2023

Based on the transfer-matrix method, we theoretically explore the transmission and reflection properties of light waves in a one-dimensional defective photonic crystal composed of superconductor (HgBa2Ca2Cu3O8+δ) and semiconductor (GaAs) layers. The whole system is centrosymmetric and can generate a defect transmission peak in the photonic band gap. We study the effect of the temperature on the defect mode. Results obtained show that the defect mode shifts to the lower frequency regions as the value of the environmental temperature increases, and the resonance of the defect mode can be strengthened further as the number of periods increases. In addition, our findings reveal that the central wavelength of the defect mode increases with the increase in the environmental temperature and it presents a nearly linear relationship between the central wavelength of the defect mode and the temperature in cryogenic environments. Therefore, we can use the temperature response of the defect mode to detect the temperature. It is hoped that this study has potential applications for the development of cryogenic sensors with high sensitivity.
Study on Surface Roughness Improvement of Selective Laser Melted Ti6Al4V Alloy
Authors: Wang Di , Lv Jiale , Wei Xiongmian , Lu Dong , Chen Chen
Published: February 2023

To improve the surface quality of Ti6Al4V parts formed by selective laser melting (SLM), this paper systematically studies the effects of laser power, scanning speed and inclination angle on the different surface morphology and roughness of parts. On this basis, the effect of surface remelting and multi-layer profile scanning process strategies on improving the surface quality of parts is explored. The upper surface roughness varies parabolically with increasing line energy density, the line energy density value that minimizes the upper surface roughness is around 0.22 J/mm, and the minimum Ra value is 4.41 μm. The roughness of upper and lower sides increases significantly with the increase in scanning speed. As the inclination angle increases, the roughness of the upper and lower sides gradually decreases, which is caused by the combined influence of powder adhesion and step effect. The surface remelting process strategy can reduce the upper surface roughness by 35.68% and reduce its Ra value to 2.65 μm. The multi-layer profile scanning process strategy can reduce the upper side and vertical side roughness by more than 50%, down to Ra 5.10 μm and Ra 4.61 μm, respectively.
Assessing Browsing Impact in Beech Forests: The Importance of Tree Responses after Browsing
Authors: Angst Janika Kim , Kupferschmid Andrea Doris
Published: February 2023

Browsing by ungulates can affect the development of a forest stand due to selective browsing and shifts in the growth ranking between tree species. Assessing browsing impact in an objective way is difficult in patchily distributed beech forests. In systematically arranged plots near Kirchberg, Switzerland, the height increment of the two saplings nearest to the plot center (k-tree method) was measured for each height class and tree species, and the within-tree browsing intensity and damage frequency were assessed. In total, 21 tree species were found. Browsing was particularly frequent in climate-adapted species. Winter browsing was more frequent than summer browsing, which was also true for deciduous species, and it significantly reduced height growth. Former damage along the main stem further reduced upgrowth. Browsing shifted the height increment ratio in favor of Fagus sylvatica. Many winter-browsed saplings of Abies alba, Fagus sylvatica, Acer pseudoplatanus, Fraxinus excelsior and Prunus avium had no new leader shoot by the end of the next growing season, i.e., browsing had a long-lasting impact. For estimating browsing impact, it is thus important to assess delays in the response after browsing. Foresters could easily apply the k-tree method in autumn for effective assessments of browsing impact.
The Missing Late Pleistocene Ostrich Femur from Zhoukoudian (China): New Information Provided by a Rediscovered Old Cast
Authors: Buffetaut Eric
Published: February 2023

A complete ostrich femur from the Late Pleistocene deposits of the Upper Cave at Zhoukoudian (China) was referred by Shaw to Struthio anderssoni in the 1930s, but its present whereabouts are unknown. A good quality plaster cast of the missing specimen has been found in the collections of the Natural History Museum (London). This cast provides interesting information about the morphology of this large ostrich femur, which had previously been only summarily described and not illustrated. Although smaller than the femora of the Early Pleistocene giant ostrich Pachystruthio, the robust femur from Zhoukoudian shows morphological similarities with them, and it is suggested that ‘Struthio’ anderssoni should be placed in the genus Pachystruthio. The importance of old palaeontological casts is emphasized, as well as the need to preserve and curate them properly.
Challenging Patterns of Atypical Dermatofibromas and Promising Diagnostic Tools for Differential Diagnosis of Malignant Lesions
Authors: Orzan Olguța Anca , Dorobanțu Alexandra Maria , Gurău Cristian Dorin , Ali Sibel , Mihai Mara Mădălina , Popa Liliana Gabriela , Giurcăneanu Călin , Tudose Irina , Bălăceanu Beatrice
Published: February 2023

Dermatofibroma (DF) or fibrous histiocytoma is one of the most frequent benign cutaneous soft-tissue lesions, characterized by a post-inflammatory tissue reaction associated with fibrosis of the dermis. Clinically DFs have a polymorphous clinical aspect from the solitary, firm, single nodules to multiple papules with a relatively smooth surface. However, multiple atypical clinicopathological variants of DFs have been reported and, therefore, clinical recognition may become challenging, leading to a more burdensome identification and sometimes to misdiagnosis. Dermoscopy is considered an important tool in DFs diagnosis, as it improves diagnostic accuracy for clinically amelanotic nodules. Although typical dermoscopic patterns are most frequently seen in clinical practice, there have also been some atypical variants described, mimicking some underlying recurrent and sometimes harmful skin afflictions. Usually, no treatment is required, although an appropriate work-up may be necessary in specific cases, such as in the presence of atypical variants or a history of recent changes. This narrative review’s aim is to summarize current evidence regarding clinical presentation, positive and differential diagnosis of atypical dermatofibromas and also to raise awareness about the importance of specific characteristics of atypical variants to better differentiate them from malignant conditions.