bluuebunny/crossref_metadata_2025 huggingface dataset:
Increased risk of psychiatric disorder in patients with hearing loss: a nationwide population-based cohort study
Authors: Nian Qun-Yi , Cheng Chun-An , Cheng Li-Hsiang , Lin Yuan-Yung , Wang Chin-Hung , Chien Wu-Chien , Chu Yueng-Hsiang , Shih Cheng-Ping , Kuo Chao-Yin , Chen Hsin-Chien , Lee Jih-Chin , Chung Chi-Hsiang , Shangkuan Wei-Chuan , Lin Hung-Che
Published: April 2024

Background

Hearing loss has been shown to be a risk factor for psychiatric disorders. In addition, long-term hearing loss is associated with increased hospitalization and mortality rates; however, the increased risk and duration of effect of hearing loss in combination with other chronic diseases on each psychiatric disorder are still not clearly defined. The purpose of this article is to clarify the risk of hearing loss for each disorder over time.

Methods

This was a retrospective cohort study, and a national health insurance research database in Taiwan was utilized. All (n = 1,949,101) Taiwanese residents who had a medical visit between 2000 and 2015 were included. Patients with hearing loss and a comparative retrospective cohort were analyzed. Every subject was tracked individually from their index date to identify the subjects who later received a diagnosis of a psychiatric disorder. The Kaplan‒Meier method was used to analyze the cumulative incidence of psychiatric disorders. Cox regression analysis was performed to identify the risk of psychiatric disorders.

Results

A total of 13,341 (15.42%) and 31,250 (9.03%) patients with and without hearing loss, respectively, were diagnosed with psychiatric disorders (P < 0.001). Multivariate analysis indicated that hearing loss significantly elevated the risk of psychiatric disorders (adjusted HR = 2.587, 95% CI 1.723–3.346, p < 0.001).

Conclusion

Our findings indicate that patients with hearing loss are more likely to develop psychiatric disorders. Furthermore, the various psychiatric disorders are more likely to occur at different times. Our findings have important clinical implications, including a need for clinicians to implement early intervention for hearing loss and to pay close attention to patients’ psychological status.

Trial registration TSGHIRB No. E202216036.
Fascin-1 limits myosin activity in microglia to control mechanical characterization of the injured spinal cord
Authors: Huang Jinxin , Hu Xuyang , Chen Zeqiang , Ouyang Fangru , Li Jianjian , Hu Yixue , Zhao Yuanzhe , Wang Jingwen , Yao Fei , Jing Juehua , Cheng Li
Published: April 2024

Background

Mechanical softening of the glial scar region regulates axonal regeneration to impede neurological recovery in central nervous system (CNS) injury. Microglia, a crucial cellular component of the glial scar, facilitate neuronal survival and neurological recovery after spinal cord injury (SCI). However, the critical mechanical characterization of injured spinal cord that harmonizes neuroprotective function of microglia remains poorly understood.

Methods

Spinal cord tissue stiffness was assessed using atomic force microscopy (AFM) in a mouse model of crush injury. Pharmacological depletion of microglia using PLX5622 was used to explore the effect of microglia on mechanical characterization. Conditional knockout of Fascin-1 in microglia (Fascin-1 CKO) alone or in combination with inhibition of myosin activity was performed to delve into relevant mechanisms of microglia regulating mechanical signal. Immunofluorescence staining was performed to evaluate the related protein levels, inflammatory cells, and neuron survival after SCI. The Basso mouse scale score was calculated to assess functional recovery.

Results

Spinal cord tissue significantly softens after SCI. Microglia depletion or Fascin-1 knockout in microglia limits tissue softening and alters mechanical characterization, which leads to increased tissue pathology and impaired functional recovery. Mechanistically, Fascin-1 inhibits myosin activation to promote microglial migration and control mechanical characterization after SCI.

Conclusions

We reveal that Fascin-1 limits myosin activity to regulate mechanical characterization after SCI, and this mechanical signal should be considered in future approaches for the treatment of CNS diseases.
SARS-CoV-2 causes dysfunction in human iPSC-derived brain microvascular endothelial cells potentially by modulating the Wnt signaling pathway
Authors: Yamada Shigeru , Hashita Tadahiro , Yanagida Shota , Sato Hiroyuki , Yasuhiko Yukuto , Okabe Kaori , Noda Takamasa , Nishida Motohiro , Matsunaga Tamihide , Kanda Yasunari
Published: April 2024

Background

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19), which is associated with various neurological symptoms, including nausea, dizziness, headache, encephalitis, and epileptic seizures. SARS-CoV-2 is considered to affect the central nervous system (CNS) by interacting with the blood–brain barrier (BBB), which is defined by tight junctions that seal paracellular gaps between brain microvascular endothelial cells (BMECs). Although SARS-CoV-2 infection of BMECs has been reported, the detailed mechanism has not been fully elucidated.

Methods

Using the original strain of SARS-CoV-2, the infection in BMECs was confirmed by a detection of intracellular RNA copy number and localization of viral particles. BMEC functions were evaluated by measuring transendothelial electrical resistance (TEER), which evaluates the integrity of tight junction dynamics, and expression levels of proinflammatory genes. BMEC signaling pathway was examined by comprehensive RNA-seq analysis.

Results

We observed that iPSC derived brain microvascular endothelial like cells (iPSC-BMELCs) were infected with SARS-CoV-2. SARS-CoV-2 infection resulted in decreased TEER. In addition, SARS-CoV-2 infection decreased expression levels of tight junction markers CLDN3 and CLDN11. SARS-CoV-2 infection also increased expression levels of proinflammatory genes, which are known to be elevated in patients with COVID-19. Furthermore, RNA-seq analysis revealed that SARS-CoV-2 dysregulated the canonical Wnt signaling pathway in iPSC-BMELCs. Modulation of the Wnt signaling by CHIR99021 partially inhibited the infection and the subsequent inflammatory responses.

Conclusion

These findings suggest that SARS-CoV-2 infection causes BBB dysfunction via Wnt signaling. Thus, iPSC-BMELCs are a useful in vitro model for elucidating COVID-19 neuropathology and drug development.
Optimization of preparation conditions for Salsola laricifolia protoplasts using response surface methodology and artificial neural network modeling
Authors: Guo Hao , Xi Yuxin , Guzailinuer Kuerban , Wen Zhibin
Published: April 2024

Background

Salsola laricifolia is a typical C3–C4 typical desert plant, belonging to the family Amaranthaceae. An efficient single-cell system is crucial to study the gene function of this plant. In this study, we optimized the experimental conditions by using Box-Behnken experimental design and Response Surface Methodology (RSM)-Artificial Neural Network (ANN) model based on the previous studies.

Results

Among the 17 experiment groups designed by Box-Behnken experimental design, the maximum yield (1.566 × 106/100 mg) and the maximum number of viable cells (1.367 × 106/100 mg) were obtained in group 12, and the maximum viability (90.81%) was obtained in group 5. Based on these results, both the RSM and ANN models were employed for evaluating the impact of experimental factors. By RSM model, cellulase R-10 content was the most influential factor on protoplast yield, followed by macerozyme R-10 content and mannitol concentration. For protoplast viability, the macerozyme R-10 content had the highest influence, followed by cellulase R-10 content and mannitol concentration. The RSM model performed better than the ANN model in predicting yield and viability. However, the ANN model showed significant improvement in predicting the number of viable cells. After comprehensive evaluation of the protoplast yield, the viability and number of viable cells, the optimal results was predicted by ANN yield model and tested. The amount of protoplast yield was 1.550 × 106/100 mg, with viability of 90.65% and the number of viable cells of 1.405 × 106/100 mg. The corresponding conditions were 1.98% cellulase R-10, 1.00% macerozyme R-10, and 0.50 mol L−1 mannitol. Using the obtained protoplasts, the reference genes (18SrRNA, β-actin and EF1-α) were screened for expression, and transformed with PEG-mediated pBI121-SaNADP-ME2-GFP plasmid vector. There was no significant difference in the expression of β-actin and EF1-α before and after treatment, suggesting that they can be used as internal reference genes in protoplast experiments. And SaNADP-ME2 localized in chloroplasts.

Conclusion

The current study validated and evaluated the effectiveness and results of RSM and ANN in optimizing the conditions for protoplast preparation using S. laricifolia as materials. These two methods can be used independently of experimental materials, making them suitable for isolating protoplasts from other plant materials. The selection of the number of viable cells as an evaluation index for protoplast experiments is based on its ability to consider both protoplast yield and viability. The findings of this study provide an efficient single-cell system for future genetic experiments in S. laricifolia and can serve as a reference method for preparing protoplasts from other materials.
Medicine, emotience, and reason
Authors: Clark John F.
Published: April 2024

Medicine is faced with a number of intractable modern challenges that can be understood in terms of hyper-intellectualization; a compassion crisis, burnout, dehumanization, and lost meaning. These challenges have roots in medical philosophy and indeed general Western philosophy by way of the historic exclusion of human emotion from human reason. The resolution of these medical challenges first requires a novel philosophic schema of human knowledge and reason that incorporates the balanced interaction of human intellect and human emotion. This schema of necessity requires a novel extension of dual-process theory into epistemology in terms of both intellect and emotion each generating a distinct natural kind of knowledge independent of the other as well as how these two forms of mental process together construct human reason. Such a novel philosophic schema is here proposed. This scheme is then applied to the practice of medicine with examples of practical applications with the goal of reformulating medical practice in a more knowledgable, balanced, and healthy way. This schema’s expanded epistemology becomes the philosophic foundation for more fully incorporating the humanities in medicine.
Application direct anterior approach in pediatric femoral head and neck lesions
Authors: Zheng Jian , Zhang Yanting , Nan Guoxin
Published: April 2024

Background

Femoral neck is one of the high-risk areas for benign tumors and tumor-like lesions. Small range of lesions may also lead to pathological fracture, femoral head necrosis and other serious problems.

Purpose

To investigate a new minimally invasive surgical approach to resect femoral head and neck lesions in children.

Patients and methods

Retrospective study of 20 patients with femoral neck and femoral head lesions from February 2019 to March 2023 in our hospital. Among them, 14 were boys and 6 were girls, 17 were femoral neck lesions and 3 were femoral head lesions. The age of the patients ranged from 3.2 to 12.6 years, with a mean of 7.1 years. The patients were divided into group A and group B according to different surgical approaches; group A used the Smith-Peterson approach, Watson-Jones approach or surgical dislocation approach and group B used the DAA. Intra-operatively, incision length, operative time and blood loss were recorded in both groups. Group A consisted of 1 femoral head lesion and 8 femoral neck lesions, including 5 cases of bone cyst and 4 cases of eosinophilic granuloma. Group B consisted of 2 femoral head lesion and 9 femoral neck lesions. A total of 11 patients with different types of disease were included in group B, including bone cysts (3 cases), aneurysmal bone cysts (1 case), eosinophilic granulomas (6 cases), Kaposi's sarcoma (1 case).

Results

The two groups of patients differed in terms of incision length (P < 0.05), operative blood loss (P < 0.05) and operative time (P < 0.05). At 6–48 months post-operatively, there were no significant differences in function and all patients had good hip function.

Conclusion

The direct anterior approach is effective for resection of paediatric femoral head and neck lesions. It provides clear exposure of the surgical site, minimal trauma and does not compromise the integrity of the anterior musculature.

Level of evidence: III.
Danggui Sini decoction alleviates oxaliplatin-induced peripheral neuropathy by regulating gut microbiota and potentially relieving neuroinflammation related metabolic disorder
Authors: Chen Chen , Xu Jian-Lin , Gu Zhan-Cheng , Zhou Shan-Shan , Wei Guo-Li , Gu Jia-Lin , Ma Hai-Long , Feng Yan-Qi , Song Zi-Wei , Yan Zhan-Peng , Deng Shan , Ding Rong , Li Song-Lin , Huo Jie-Ge
Published: April 2024

Background

Danggui Sini decoction (DSD), a traditional Chinese medicine formula, has the function of nourishing blood, warming meridians, and unblocking collaterals. Our clinical and animal studies had shown that DSD can effectively protect against oxaliplatin (OXA)-induced peripheral neuropathy (OIPN), but the detailed mechanisms remain uncertain. Multiple studies have confirmed that gut microbiota plays a crucial role in the development of OIPN. In this study, the potential mechanism of protective effect of DSD against OIPN by regulating gut microbiota was investigated.

Methods

The neuroprotective effects of DSD against OIPN were examined on a rat model of OIPN by determining mechanical allodynia, biological features of dorsal root ganglia (DRG) as well as proinflammatory indicators. Gut microbiota dysbiosis was characterized using 16S rDNA gene sequencing and metabolism disorders were evaluated using untargeted and targeted metabolomics. Moreover the gut microbiota mediated mechanisms were validated by antibiotic intervention and fecal microbiota transplantation.

Results

DSD treatment significantly alleviated OIPN symptoms by relieving mechanical allodynia, preserving DRG integrity and reducing proinflammatory indicators lipopolysaccharide (LPS), IL-6 and TNF-α. Besides, DSD restored OXA induced intestinal barrier disruption, gut microbiota dysbiosis as well as systemic metabolic disorders. Correlation analysis revealed that DSD increased bacterial genera such as Faecalibaculum, Allobaculum, Dubosiella and Rhodospirillales_unclassified were closely associated with neuroinflammation related metabolites, including positively with short-chain fatty acids (SCFAs) and sphingomyelin (d18:1/16:0), and negatively with pi-methylimidazoleacetic acid, l-glutamine and homovanillic acid. Meanwhile, antibiotic intervention apparently relieved OIPN symptoms. Furthermore, fecal microbiota transplantation further confirmed the mediated effects of gut microbiota.

Conclusion

DSD alleviates OIPN by regulating gut microbiota and potentially relieving neuroinflammation related metabolic disorder.
Application of immersive virtual reality in the training of wheelchair boxers: evaluation of exercise intensity and users experience additional load– a pilot exploratory study
Authors: Polechoński Jacek , Langer Alan , Akbaş Anna , Zwierzchowska Anna
Published: April 2024

Background

Over the last few years, there has been a growing interest in workout apps and active virtual reality video games (AVRGs), which provide entertainment and enable users to undertake various forms of physical activity (PA) at home. Presumably, these types of exercises can be particularly useful for people with physical disabilities, who experience problems with access to sports and leisure facilities due to architectural and communication barriers. However, it is interesting whether the intensity of PA in VR is high enough to provide users with health benefits, as it is mainly based on arm movements.

Objective

The main aim of the study was to evaluate the intensity of physical exercise of wheelchair boxers during a boxing training session using the FitXR app in immersive VR in light of health-related PA recommendations. The effect of Velcro-fastened hand-held weights (HHWs) on the intensity of PA undertaken by people in VR was also examined, and the attractiveness of virtual exercise were assessed in the opinion of users.

Methods

PA intensity was evaluated using a heart rate monitor based on the percentage of maximal heart rate (% HRmax) and the Borg’s rating of perceived exertion (RPE 6–20). The attractiveness perceived during exercise by users were evaluated using the Physical Activity Enjoyment Scale (PACES 1–7 scale).

Results

The study shows that the exercise intensity of the athletes during wheelchair boxing training in VR is at a beneficial moderate level for health (HRave=68.98% HRmax). The use of HHWs (0.5 kg) does not significantly increase the PA intensity of the individuals during virtual exercise. Users with disabilities highly rated the attractiveness (6.32 ± 0.79 points) of PA during virtual boxing training.

Conclusions

Boxing exercises in VR can be an attractive and health-related form of PA for wheelchair boxers and a supplement to their conventional training.
Comprehensive machine learning boosts structure-based virtual screening for PARP1 inhibitors
Authors: Caba Klaudia , Tran-Nguyen Viet-Khoa , Rahman Taufiq , Ballester Pedro J.
Published: April 2024

Poly ADP-ribose polymerase 1 (PARP1) is an attractive therapeutic target for cancer treatment. Machine-learning scoring functions constitute a promising approach to discovering novel PARP1 inhibitors. Cutting-edge PARP1-specific machine-learning scoring functions were investigated using semi-synthetic training data from docking activity-labelled molecules: known PARP1 inhibitors, hard-to-discriminate decoys property-matched to them with generative graph neural networks and confirmed inactives. We further made test sets harder by including only molecules dissimilar to those in the training set. Comprehensive analysis of these datasets using five supervised learning algorithms, and protein–ligand fingerprints extracted from docking poses and ligand only features revealed one highly predictive scoring function. This is the PARP1-specific support vector machine-based regressor, when employing PLEC fingerprints, which achieved a high Normalized Enrichment Factor at the top 1% on the hardest test set (NEF1% = 0.588, median of 10 repetitions), and was more predictive than any other investigated scoring function, especially the classical scoring function employed as baseline.
Klotho exerts protection in chronic kidney disease associated with regulating inflammatory response and lipid metabolism
Authors: Liu Junhui , Wang Huaicheng , Liu Qinyu , Long Shushu , Wu Yanfang , Wang Nengying , Lin Wei , Chen Gang , Lin Miao , Wen Junping
Published: April 2024

Background

The anti-aging protein Klotho plays a protective role in kidney disease, but its potential as a biomarker for chronic kidney disease (CKD) is controversial. Additionally, the main pathways through which Klotho exerts its effects on CKD remain unclear. Therefore, we used bioinformatics and clinical data analysis to determine its role in CKD.

Results

We analyzed the transcriptomic and clinical data from the Nephroseq v5 database and found that the Klotho gene was mainly expressed in the tubulointerstitium, and its expression was significantly positively correlated with estimated glomerular filtration rate (eGFR) and negatively correlated with blood urea nitrogen (BUN) in CKD. We further found that Klotho gene expression was mainly negatively associated with inflammatory response and positively associated with lipid metabolism in CKD tubulointerstitium by analyzing two large sample-size CKD tubulointerstitial transcriptome datasets. By analyzing 10-year clinical data from the National Health and Nutrition Examination Survey (NHANES) 2007–2016, we also found that Klotho negatively correlated with inflammatory biomarkers and triglyceride and positively correlated with eGFR in the CKD population. Mediation analysis showed that Klotho could improve renal function in the general population by modulating the inflammatory response and lipid metabolism, while in the CKD population, it primarily manifested by mediating the inflammatory response. Restricted cubic spline (RCS) analysis showed that the optimal concentration range for Klotho to exert its biological function was around 1000 pg/ml. Kaplan–Meier curves showed that lower cumulative hazards of all-cause mortality in participants with higher levels of Klotho. We also demonstrated that Klotho could reduce cellular inflammatory response and improve cellular lipid metabolism by establishing an in vitro model similar to CKD.

Conclusions

Our results suggest that Klotho exerts protection in CKD, which may be mainly related to the regulation of inflammatory response and lipid metabolism, and it can serve as a potential biomarker for CKD.
Continuous immunosuppression is required for suppressing immune responses to xenografts in non-human primate brains
Authors: Feng Su , Zhang Ting , He Zhengxiao , Zhang Wenchang , Chen Yingying , Yue Chunmei , Jing Naihe
Published: April 2024

Continuous immunosuppression has been widely used in xenografts into non-human primate brains. However, how immune responses change after transplantation in host brains under continuous immunosuppressive administration and whether immunosuppression can be withdrawn to mitigate side effects remain unclear. Human induced neural stem/progenitor cells (iNPCs) have shown long-term survival and efficient neuronal differentiation in primate brains. Here, we evaluate the immune responses in primate brains triggered by human grafts. The results show that the immune responses, including the evident activation of microglia and the strong infiltration of lymphocytes (both T- and B-cells), are caused by xenografts at 4 months post transplantation (p.t.), but significantly reduced at 8 months p.t. under continuous administration of immunosuppressant Cyclosporin A. However, early immunosuppressant withdrawal at 5 months p.t. results in severe immune responses at 10 months p.t. These results suggest that continuous long-term immunosuppression is required for suppressing immune responses to xenografts in primate brains.
Evaluation on Properties of Cement Mortar and Brick Using Magnetically Separated Coal Power Plant Bottom Ash
Authors: Kim Ji-Hyun , Moon Hoon , Chung Chul-Woo
Published: April 2024

Recycling of abandoned waste bottom ash has been a key issue in Republic of Korea in terms of environmental protection as well as economic concern. In this work, a method for recycling of abandoned bottom ash has been discussed based on the results from laboratory and industrial-scale experiments. Abandoned bottom ash was magnetically separated and properties of magnetically separated bottom ash samples as well as properties of mortar and masonry cement brick made of bottom ash were investigated. According to the experimental results, bulk and skeletal densities were ranked in the order of strongly magnetic > weakly magnetic > as-received > non-magnetic (from heavier to lighter) bottom ash. From laboratory-scale experiments, compressive strengths of mortars made of bottom ash samples (measured by ASTM C 109) were lower than that of mortar made of standard sand. Among bottom ash samples, mortar made of non-magnetic bottom ash (after removal of unburnt carbon) showed higher compressive strength with lower thermal conductivity (measured by ASTM C 1113) and weight than others. Masonry cement brick made of magnetic bottom ash showed lower weight and thermal conductivity than those made of standard sand, while meeting the KS strength guideline as a masonry cement brick. The results suggest the applicability of bottom ash as lightweight aggregate for production of masonry cement brick. However, considering the lower strength obtained from masonry cement brick made of as-received bottom ash (without removal of unburnt carbon), unburnt carbon content should be removed prior to its utilization as lightweight aggregate.
Probiotic Pediococcus pentosaceus restored gossypol-induced intestinal barrier injury by increasing propionate content in Nile tilapia
Authors: Ding Feifei , Zhou Nannan , Luo Yuan , Wang Tong , Li Weijie , Qiao Fang , Du Zhenyu , Zhang Meiling
Published: April 2024

Background

Intestinal barrier is a dynamic interface between the body and the ingested food components, however, dietary components or xenobiotics could compromise intestinal integrity, causing health risks to the host. Gossypol, a toxic component in cottonseed meal (CSM), caused intestinal injury in fish or other monogastric animals. It has been demonstrated that probiotics administration benefits the intestinal barrier integrity, but the efficacy of probiotics in maintaining intestinal health when the host is exposed to gossypol remains unclear. Here, a strain (YC) affiliated to Pediococcus pentosaceus was isolated from the gut of Nile tilapia (Oreochromis niloticus) and its potential to repair gossypol-induced intestinal damage was evaluated.

Results

A total of 270 Nile tilapia (2.20 ± 0.02 g) were allotted in 3 groups with 3 tanks each and fed with 3 diets including CON (control diet), GOS (control diet containing 300 mg/kg gossypol) and GP (control diet containing 300 mg/kg gossypol and 108 colony-forming unit (CFU)/g P. pentosaceus YC), respectively. After 10 weeks, addition of P. pentosaceus YC restored growth retardation and intestinal injury induced by gossypol in Nile tilapia. Transcriptome analysis and siRNA interference experiments demonstrated that NOD-like receptors (NLR) family caspase recruitment domain (CARD) domain containing 3 (Nlrc3) inhibition might promote intestinal stem cell (ISC) proliferation, as well as maintaining gut barrier integrity. 16S rRNA sequencing and gas chromatography-mass spectrometry (GC-MS) revealed that addition of P. pentosaceus YC altered the composition of gut microbiota and increased the content of propionate in fish gut. In vitro studies on propionate’s function demonstrated that it suppressed nlrc3 expression and promoted wound healing in Caco-2 cell model.

Conclusions

The present study reveals that P. pentosaceus YC has the capacity to ameliorate intestinal barrier injury by modulating gut microbiota composition and elevating propionate level. This finding offers a promising strategy for the feed industry to incorporate cottonseed meal into fish feed formulations.

Graphical Abstract
Exploring the connotation of “Local Community” in ICOMOS discourse: a textual analysis based on authoritative documents
Authors: Xu Yishu , Tang Yuexing , Zhang Luchen
Published: April 2024

As an authoritative institution in cultural heritage conservation, ICOMOS plays a crucial role in guiding local communities’ participation in heritage conservation. However, its scattered and vague descriptions of local communities pose significant obstacles to further research and practice of community participation in heritage conservation. Given the increasing importance of local communities in heritage conservation, it is essential to systematically explore the connotation of Local Community connotations within ICOMOS discourse. This research employs Natural Language Processing methods to analyze ICOMOS’s descriptions of Local Community. Utilizing computational techniques of word frequency calculation, LDA (Latent Dirichlet Allocation) topic model keyword calculation, and hierarchical clustering calculation, the research uncovers relevant keywords and its thematic clusters of Local Community. These findings are further elucidated by aligning them with the principles outlined in authoritative documents of ICOMOS. The research indicates that ICOMOS’s descriptions of Local Community can be summarized into four main keywords families. These keywords families can be summarized as a comprehensive Local Community “three-level, four-family” keywords system. The “Tourism” keywords family exhibits a close association with Local Community, highlighting ICOMOS’s heightened emphasis on heritage tourism. The “Management-Development” keywords family occupies the second level, emphasizing fundamental principles for local communities’ participation in heritage practices. The “Traditional-Knowledge” and “Social-Economic” Keywords Families, situated in the third level, respectively describe the value attributes and conservation approaches of local communities. Each keywords family formed over different periods, exhibiting varying trends of development. By systematically integrating ICOMOS’s descriptions of Local Community and employing Natural Language Processing for in-depth exploration, This research aims to construct a cognitive understanding of local communities from a new perspective of quantitative text analysis, with the intention of providing theoretical references for subsequent research on local communities.
Identifying factors influencing the spatial distribution of minority cultural heritage in Southwest China
Authors: Li Chang , Qian Yuyao , Li Zhaokun , Tong Tong
Published: April 2024

Minority Cultural Heritage (MCH) plays a crucial role in preserving human cultural and historical diversity. In Southwest China, there is a disparity between the abundance of ethnic minority cultural heritage resources and their inadequate protection and development. However, limited by interdisciplinary barriers, research into the distribution patterns of varied MCH in this area remains unexplored, making an initial step towards comprehensive preservation. The study investigates the relationship between MCH distribution and its association with factors of geography, climate, transportation, economics, and demographics, utilizing spatial geographic analysis, Geodetector methods, and social network analysis. The results indicate the following: (1) The distribution of 483 national-level MCH in Southwest China exhibits clustering, with a higher concentration in the southern regions. Honghe Prefecture, Dali Prefecture, and Qiandongnan Prefecture serve as the core areas with high MCH density, accounting for 47.2% of the total concentration. (2) All three categories of MCH show cohesive distribution patterns. cultural heritage clusters in the northern to southeastern regions of Yunnan Province, intangible cultural heritage clusters in the southeastern to southern regions of Guizhou Province, and agricultural cultural heritage clusters in the northern region of Yunnan Province. (3) The distribution of MCH among different ethnic minorities demonstrates polarization. The Yi and Tibetan ethnic groups have a higher quantity and diversity of MCH, while the Qiang, Lisu, and Jingpo ethnic groups have relatively fewer resources. (4) Single-factor analysis reveals that natural factors like the proportion of mountainous areas, river density, and annual average sunshine, as well as human factors like the proportion of minority population, urbanization rate, and road mileage, have the strongest explanatory power for the distribution of MCH. Furthermore, the interaction between these factors and others enhances the explanatory power for the distribution of MCH in Southwest China. This study provides scientific evidence for the assessment, protection, and sustainable development of MCH.
Special issue “VLF/ELF remote sensing of ionospheres and magnetospheres”
Authors: Omura Yoshiharu , Bortnik Jacob , Clilverd Mark , Demekhov Andrei , Miyake Yohei
Published: April 2024

Graphical Abstract
Distribution of eukaryotic environmental DNA in global subseafloor sediments
Authors: Hoshino Tatsuhiko , Inagaki Fumio
Published: April 2024

The analysis of eukaryotic environmental DNA (eDNA) in sediment has the potential for understanding past ecosystems, even for taxa lacking skeletons or preserved only as a part of necromass. Despite the paleoenvironmental and ecological importance of eukaryotic eDNA in marine sediment, the duration of remaining eDNA and the species of eDNA present in marine sediment has not been well investigated. Here, we analyzed eDNA extracted from 299 sediment samples down to 678 m below the seafloor at 40 geologically distinct sites. The results showed that eukaryotic eDNA was amplified from more than 80% of the sediments with a depositional age of less than 100,000 years. The eDNA was well conserved in anoxic sediments than in oxic sediments, with PCR success rates of 48% and 18%, respectively. The eukaryotic communities include non-benthic organisms such as marine plankton, including diatoms, dinoflagellates, and coccolithophores. A freshwater diatom genus Aulacoseira was detected in the Baltic Sea sediments from the last glacial lacustrine environment. These results provide new insights into the global-scale distribution of the past eukaryotic eDNA preserved in marine sediment.
A case of gastric adenocarcinoma with pyloric gland-type infiltrating submucosa
Authors: Hirata Kaiho , Yagi Shusuke , Miyazaki Hideki , Yamada Kazuhiko , Akazawa Naoki , Enomoto Naoki , Nohara Kyoko , Yokoi Chizu , Igari Toru , Kokudo Norihiro
Published: April 2024

Background

The development of immunohistochemical staining has revealed that gastric adenocarcinoma with the gastric phenotype can be divided into the foveolar, fundic gland, and pyloric gland phenotypes. Gastric adenocarcinoma of the pyloric gland type is difficult to diagnose using biopsy because of its low atypia and rarity. Herein, we describe a case of gastric adenocarcinoma of the pyloric gland type that was diagnosed immunohistochemically after endoscopic resection.

Case presentation

A 67-year-old man was referred to our hospital for the diagnosis and treatment of a 30-mm elevated lesion on the lesser curvature side of the middle of the gastric body. Although four biopsies were performed, it was difficult to determine whether the lesion was benign or malignant. Therefore, endoscopic submucosal dissection was performed, and the presence of tumor cells infiltrating the submucosa with venous invasions was identified. Immunohistochemical staining revealed that the tumor cells were positive for MUC5AC and MUC6 and negative for Pepsinogen I and H + /K + -ATPase. From the above findings, he was diagnosed as having gastric adenocarcinoma with pyloric gland type. The patient underwent a laparoscopic distal gastrectomy and was discharged without any adverse events.

Conclusions

Gastric adenocarcinoma of the pyloric gland type is a rare disease, and endoscopic resection can serve as a viable diagnostic option for this condition when it is difficult to diagnose using biopsy. Immunohistochemical pathology images can aid in the diagnosis of gastric adenocarcinoma of the pyloric gland type.
Parenting practices and interventions during the COVID-19 pandemic lockdown: an exploratory cross-sectional study of caregivers in Brazil, Mexico, and the United States
Authors: Juras Mariana M. , Coelho Acileide Cristiane F. , Vázquez Alejandro L. , Ribeiro Michela , Kohlsdorf Marina , Custódio Alice Lima , Amador Buenabad Nancy G. , Perez Lucia Vazquez , Hooley Cole , Barnett Miya L. , Baumann Ana A.
Published: April 2024

Introduction

The COVID-19 pandemic led countries’ governments to rapidly establish lockdowns and social distancing, which altered family routines and the quality of family relationships worldwide.

Objectives

This exploratory cross-sectional study aimed to identify the impacts of the social distancing and lockdown in parenting practices of caregivers from Brazil, Mexico, and the USA, and to analyze the continuity of parenting intervention support for children and their families at the beginning of the pandemic in these countries.

Methods

The sample consisted of 704 caregivers of children (286 from Brazil, 225 from Mexico, and 193 from the USA) who answered an online survey about parenting practices before/after quarantine, caregiver/child routines, feelings related to quarantine, changes in everyday life since the beginning of the COVID-19 pandemic, contact with health professionals, and sources of parenting information.

Results

Data indicate that caregivers from the three countries experienced similar parenting practices during this time, and did not report significant changes before and after the lockdown. They sought information about parenting predominantly via social media. Those receiving previous mental health care perceived the transition from in-person to telehealth services during the pandemic as feasible and acceptable.

Conclusion

This study will be helpful for clinicians and parents to contextualize their practices amid long-standing effects that the COVID-19 pandemic can have on children and their families during and post-pandemic from multiple cultural backgrounds.
Who speaks for the university? Social fiction as a lens for reimagining higher education futures
Authors: Mishra Punya , Oster Nicole , Wagner Phoebe
Published: April 2024

This paper combines social fiction and academic analysis to envision hopeful futures for higher education. At the heart of the exploration is Phoebe Wagner’s speculative fiction piece, University, Speaking, which personifies a university grappling with environmental, political, and social change. Phoebe Wagner’s first-person narrative highlights the power of collective voice, the importance of centering community, and the urgent need to cultivate resilience and adaptability. Through analysis of key themes, this paper connects Phoebe Wagner’s fictional vision to contemporary research on the multi-faceted and complex challenges facing universities today. By integrating artistic and academic perspectives, this paper discusses new possibilities for universities navigating disruption and change.
Access to essential medicines for diabetes care: availability, price, and affordability in central Ethiopia
Authors: Deressa Hachalu Dugasa , Abuye Habtamu , Adinew Alemayehu , Ali Mohammed K. , Kebede Tedla , Habte Bruck Messele
Published: April 2024

Background

Diabetes is a major global public health burden. Effective diabetes management is highly dependent on the availability of affordable and quality-assured essential medicines (EMs) which is a challenge especially in low-and-middle-income countries such as Ethiopia. This study aimed to assess the accessibility of EMs used for diabetes care in central Ethiopia’s public and private medicine outlets with respect to availability and affordability parameters.

Methods

A cross-sectional study was conducted in 60 selected public and private medicine outlets in central Ethiopia from January to February 2022 using the World Health Organization/Health Action International (WHO/HAI) standard tool to assess access to EMs. We included EMs that lower glucose, blood pressure, and cholesterol as these are all critical for diabetes care. Availability was determined as the percentage of surveyed outlets per sector in which the selected lowest-priced generic (LPG) and originator brand (OB) products were found. The number of days’ wages required by the lowest paid government worker (LPGW) to purchase a one month’s supply of medicines was used to measure affordability while median price was determined to assess patient price and price markup difference between public procurement and retail prices.

Results

Across all facilities, availability of LPG and OB medicines were 34.6% and 2.5% respectively. Only two glucose-lowering (glibenclamide 5 mg and metformin 500 mg) and two blood pressure-lowering medications (nifedipine 20 mg and hydrochlorothiazide 25 mg) surpassed the WHO’s target of 80% availability. The median price based on the least measurable unit of LPG diabetes EMs was 1.6 ETB (0.033 USD) in public and 4.65 ETB (0.095 USD) in private outlets. The cost of one month’s supply of diabetes EMs was equivalent to 0.3 to 3.1 days wages in public and 1.0 to 11.0 days wages in private outlets, respectively, for a typical LPGW. Thus, 58.8% and 84.6% of LPG diabetes EMs included in the price analysis were unaffordable in private and public outlets, respectively.

Conclusions

There are big gaps in availability and affordability of EMs used for diabetes in central Ethiopia. Policy makers should work to improve access to diabetes EMs. It is recommended to increase government attention to availing affordable EMs for diabetes care including at the primary healthcare levels which are more accessible to the majority of the population. Similar studies are also recommended to be conducted in different parts of Ethiopia.
Concepts of illness etiology in a traditional medical system: analysis of philosophy of Aruh and healing ritual as ethnomedicine
Authors: Anshari Muhammad
Published: April 2024

The process of recovering from a disease can vary among ethnic groups, culminating in distinct medical systems. Indonesia, an archipelagic country with over a thousand ethnicities, requires extensive research on ethnomedicine. A critical aspect of this research is illness etiology, which plays a crucial role in understanding the concept of prevention, therapy, and treatment developed by these ethnic groups. An adequate understanding of illness etiology will facilitate formulating a powerful, superior traditional medical system and provide practical benefits to assist local community health efforts. Therefore, this research aims to describe illness etiology by analyzing the philosophy of life: aruh and healing ritual according to the ethnomedicine of the Dayak Paramasan in Meratus Mountains. The method used was ethnography, which involved two traditional healing practitioners. Based on the results, illness were caused by behavior that was not aruh (disharmonious) with the environment. The types of illness include alamiah (physiological), perbuatan (psychosocial), and kapuhunan (socio-cultural). Meanwhile, illness therapy with ritual aims to return the aruh (harmonious, intimate, and peaceful) living conditions between the soul of the sufferer and the natural environment.
Evaluation of Artemisia dubia folium extract-mediated immune efficacy through developing a murine model for acute and chronic stages of atopic dermatitis
Authors: Acharya Manju , Gautam Ravi , Yang SuJeong , Jo JiHun , Maharjan Anju , Lee DaEun , Ghimire Narayan Prasad , Min ByeongSun , Kim ChangYul , Kim HyoungAh , Heo Yong
Published: April 2024

Background

Atopic dermatitis (AD) is a biphasic type of skin inflammation characterized by a predominance of type-2 (TH2) and type-1 (TH1) helper T cell-biased immune responses at the acute and persistent chronic phases, respectively. The present study was aimed to evaluate the efficacy of Artemisia dubia folium extract (ADFE) on AD-like skin lesions through developing a murine model for acute and chronic stages of AD. To induce acute phase AD, the dorsal skin of BALB/c mice was sensitized twice a week with 1% 2, 4-dinitrochlorobenzene (DNCB), followed by challenge (twice) in the following week with 0.2% DNCB. To induce persistent chronic AD, some mice were challenged twice a week for 4 more weeks. After the second challenge, the dorsal skin was exposed to 3% ADFE (five times per week) for 2 weeks (acute phase) or 4 weeks (persistent chronic phase).

Results

The paradigm of TH2 or TH1 predominance at the acute and chronic phase, respectively, was observed in this mouse model. During the acute phase, we observed an increased IL-4/IFN-γ ratio in splenic culture supernatants, an increased IgG1/IgG2a ratio in serum, and elevated serum IgE levels; however, the skew toward TH2 responses was diminished during the chronic stage. Compared with vehicle controls, ADFE reduced the IL-4/IFN-γ and IgG1/IgG2a ratios in acute AD, but both ratios increased during the chronic stage.

Conclusions

Our results suggest that ADFE concomitantly suppresses the TH2 predominant response in acute AD, as well as the TH1 predominant response in chronic AD. Thus, ADFE is a candidate therapeutic for AD.
Adjunctive repetitive transcranial magnetic stimulation therapy’s effectiveness in treating a sample of patients with major depressive disorder refractory to first-line drug treatment
Authors: Javadi Alireza Haji Seyed , Mohammadian Abdul Rasool , Shafikhani Ali Akbar
Published: April 2024

Background

The usefulness of repetitious transcranial magnetic stimulation (rTMS) and its protocols in the treatment of major depressive disorder (MDD) remains controversial. This study aimed to evaluate the efficacy of rTMS in treating a sample of patients with MDD who did not respond to conventional treatment.

Results

The Hamilton Depression Rating Scale (HDRS) scores for the active rTMS group were 19.66 ± 6.70 at baseline, 12.50 ± 6.69 at 2 weeks, and 11.23 ± 6.59 at 4 weeks. The average HDRS scores for the sham rTMS group were 20.03 ± 7.40 at baseline, 19.36 ± 6.86 at 2 weeks, and 18.53 ± 7.10 at 4 weeks (F = 5.98; p < 0.01). The Clinical Global Impression-Severity Scale (CGI-S) scores were significantly lower in the second and fourth weeks than the baseline due to the significant interaction between time effects and the groups (F = 9.95, p = 0.002). This condition was also similar to the CGI-Improvement Scale and Brief Illness Perception Questionnaire (Brief IPQ), and the intervention group showed a significantly lower score than the control group (p < 0.05).

Conclusions

This study showed that rTMS using the employed protocol was promising for patients with MDD resistant to first-line drug therapy. Further studies are required to ensure our observation.

Trial registration

Trial registration number: IRCT20190612043877N1

Trial registry Record URL: https://irct.behdasht.gov.ir/trial/63919
Expression of serum microRNAs, mir-182-5p, and miR-590-3p and its clinical significance in neonatal sepsis
Authors: Hamdy Soha M. , Othman Yomna A. , Abdelaleem Omayma O. , Abd El-Hamid Rehab G. , Ali Doaa Y.
Published: April 2024

Background

Neonatal sepsis is one of the life-threatening diseases. MicroRNAs are non-coding RNAs that play vital roles in various diseases.

Methods

This study included 50 neonates with sepsis and 60 healthy controls. RNA extraction and assessment of mir-182-5p and miR-590-3p using real-time PCR were done.

Results

Significant downregulation of mir-182-5p and miR-590-3p in neonates with sepsis compared with healthy neonates was observed. Positive correlations were confirmed between the expression levels of miR-182-5p and birth weight (R = 0.355, P = 0.012), RDW (R = 0.476, p =  < 0.0001), I/T Neutrophil (R = 0.362, P = 0.012), and a negative correlations were demonstrated between miR-182-5p and each of lyomphocyte count (R =  − 0.399, P = 0.004), HCO3 (R =  − 0.396, P = 0.004), as well as snap score (R =  − 0.321, P = 0.023). Moreover, positive correlations were verified between the expression level of miR-590-3p and I/T Neutrophil (R = 0.420, P = 0.003), RDW (R = 0.359, p = 0.010), CRP (R = 0.285, P = 0.45), and negative correlations were established between the expression level of miR-590-3p and platelets (R =  − 0.495, P =  < 0.0001), lymphocyte count (R =  − 0.365, P = 0.009), and snap score (R =  − 0.568, P =  < 0.0001).

Conclusion

mir-182-5p and miR-590-3p may be used as new biomarkers for neonatal sepsis suggesting that they could be used in the treatment of neonatal sepsis. Also, a significant negative correlation was noted between expression levels of mir-182-5p and miR-590-3p and snap score.