bluuebunny/crossref_metadata_2025 huggingface dataset:
Phycoremediated Microalgae and Cyanobacteria Biomass as Biofertilizer for Sustainable Agriculture: A Holistic Biorefinery Approach to Promote Circular Bioeconomy
Authors: Renganathan Prabhaharan , Gaysina Lira A. , Holguín-Peña Ramón Jaime , Sainz-Hernández Juan Carlos , Ortega-García Jesus , Rueda-Puente Edgar Omar
Published: September 2024

The increasing global population has raised concerns about meeting growing food demand. Consequently, the agricultural sector relies heavily on chemical fertilizers to enhance crop production. However, the extensive use of chemical fertilizers can disrupt the natural balance of the soil, causing structural damage and changes in the soil microbiota, as well as affecting crop yield and quality. Biofertilizers and biostimulants derived from microalgae and cyanobacteria are promising sustainable alternatives that significantly influence plant growth and soil health owing to the production of diverse biomolecules, such as N-fixing enzymes, phytohormones, polysaccharides, and soluble amino acids. Despite these benefits, naturally producing high-quality microalgal biomass is challenging owing to various environmental factors. Controlled settings, such as artificial lighting and photobioreactors, allow continuous biomass production, but high capital and energy costs impede large-scale production of microalgal biomass. Sustainable methods, such as wastewater bioremediation and biorefinery strategies, are potential opportunities to overcome these challenges. This review comprehensively summarizes the plant growth-promoting activities of microalgae and elucidates the mechanisms by which various microalgal metabolites serve as biostimulants and their effects on plants, using distinct application methods. Furthermore, it addresses the challenges of biomass production in wastewater and explores biorefinery strategies for enhancing the sustainability of biofertilizers.
Influence of Genetic Polymorphisms on the Age at Cancer Diagnosis in a Homogenous Lynch Syndrome Cohort of Individuals Carrying the MLH1:c.1528C>T South African Founder Variant
Authors: Ndou Lutricia , Chambuso Ramadhani , Algar Ursula , Goldberg Paul , Boutall Adam , Ramesar Raj
Published: September 2024

Background: High variability in the age at cancer diagnosis in Lynch syndrome (LS) patients is widely observed, even among relatives with the same germline pathogenic variant (PV) in the mismatch repair (MMR) genes. Genetic polymorphisms and lifestyle factors are thought to contribute to this variability. We investigated the influence of previously reported genetic polymorphisms on the age at cancer diagnosis in a homogenous LS cohort with a South African founder germline PV c.1528C>T in the MLH1 gene. Methods: A total of 359 LS variant heterozygotes (LSVH) from 60 different families were genotyped for specific genetic polymorphisms in GSTM1, GSTT1, CYP1A1, CYP17, PPP2R2B, KIF20A, TGFB1, XRCC5, TNF, BCL2, CHFR, CDC25C, ATM, TTC28, CDC25C, HFE, and hTERT genes using Multiplex Polymerase Chain Reaction and MassArray methods. Kaplan–Meier survival analysis, univariate and multivariate Cox proportional hazards gamma shared frailty models adjusted for sex were used to estimate the association between age at cancer diagnosis and polymorphism genotypes. A p-value < 0.05 after correcting for multiple testing using the Benjamini–Hochberg method was considered significant at a 95% confidence interval. Results: We identified three genotypes in the cell-cycle regulation, DNA repair, and xenobiotic-metabolism genes significantly associated with age at cancer diagnosis in this cohort. The CYP1A1 rs4646903 risk (GG) and CDC25C rs3734166 polymorphic (GA+AA) genotypes were significantly associated with an increased risk of a younger age at cancer diagnosis (Adj HR: 2.03 [1.01–4.08], p = 0.034 and Adj HR: 1.53 [1.09–2.14], p = 0.015, respectively). LSVH who were heterozygous for the XRCC5 rs1051685 SNP showed significant protection against younger age at cancer diagnosis (Adj HR: 0.69 [CI, 0.48–0.99], p = 0.043). The risk of a younger age at any cancer diagnosis was significantly high in LS carriers of one to two risk genotypes (Adj HR: 1.49 [CI: 1.06–2.09], corrected p = 0.030), while having one to two protective genotypes significantly reduced the risk of developing any cancer and CRC at a younger age (Adj HR: 0.52 [CI: 0.37–0.73], and Adj HR: 0.51 [CI: 0.36–0.74], both corrected p < 0.001). Conclusions: Polymorphism genotypes in the cell-cycle regulation, DNA repair, and xenobiotic metabolizing genes may influence the age at cancer diagnosis in a homogenous LS cohort with a South African founder germline PV c.1528C>T in the MLH1 gene.
The Functional and Prognostic Impact of TIGIT Expression on Bone Marrow NK Cells in Core Binding Factor-Acute Myeloid Leukemia Patients at Diagnosis
Authors: Xie Dai-Hong , Wang Jun , Sun Kai , Shi Zong-Yan , Wang Ya-Zhe , Chang Yan , Yuan Xiao-Ying , Liu Yan-Rong , Jiang Hao , Jiang Qian , Huang Xiao-Jun , Qin Ya-Zhen
Published: September 2024

Background: The effect of the expression of the newly identified immune checkpoint, T cell immunoglobulin and immunoreceptor tyrosine-based inhibition motif domain (TIGIT) on NK cells in core binding factor-acute myeloid leukemia (CBF-AML) remains to be investigated. Methods: Fresh bone marrow samples from a total of 39 newly diagnosed CBF-AML patients and 25 healthy donors (HDs) were collected for testing the phenotype and function state of total NK, CD56bright, and CD56dim NK cell subsets after in vitro stimulation. Results: The frequencies of TIGIT+ cells in total NK, CD56bright, and CD56dim NK cell subsets had no significant difference between patients and HDs. TNF-α and INF-γ levels were uniformly lower in TIGIT+ cells than the corresponding TIGIT− cells in all HDs, whereas those for TIGIT+ to TIGIT− cells in patients were highly heterogenous; TIGIT expression was not related to PFP and GZMB expression in HDs, whereas it was related to higher intracellular PFP and GZMB levels in patients. Patients’ TIGIT+ NK cells displayed lower K562 cell-killing activity than their TIGIT− NK cells. In addition, high frequencies of TIGIT+ cells in total NK and CD56dim NK cells were associated with poor RFS. Conclusions: TIGIT expression affected the diagnostic bone marrow-sited NK cell function and had prognostic significance in CBF-AML patients.
Set Packing Optimization by Evolutionary Algorithms with Theoretical Guarantees
Authors: Jin Youzhen , Xia Xiaoyun , Wang Zijia , Peng Xue , Zhang Jun , Liao Weizhi
Published: September 2024

The set packing problem is a core NP-complete combinatorial optimization problem which aims to find the maximum collection of disjoint sets from a given collection of sets, S, over a ground set, U. Evolutionary algorithms (EAs) have been widely used as general-purpose global optimization methods and have shown promising performance for the set packing problem. While most previous studies are mainly based on experimentation, there is little theoretical investigation available in this area. In this study, we analyze the approximation performance of simplified versions of EAs, specifically the (1+1) EA, for the set packing problem from a theoretical perspective. Our analysis demonstrates that the (1+1) EA can provide an approximation guarantee in solving the k-set packing problem. Additionally, we construct a problem instance and prove that the (1+1) EA beats the local search algorithm on this specific instance. This proof reveals that evolutionary algorithms can have theoretical guarantees for solving NP-hard optimization problems.
Modeling Thermal Effects of Pulsating Currents in Human Tissues: How to Prevent Necrosis
Authors: Farina Angiolo , Fasano Antonio , Rosso Fabio
Published: September 2024

In certain clinical applications, pulsating currents are applied to specific body regions for therapeutic purposes. In this paper, we analyze the resulting thermal field to determine the optimal amplitude, period, and duration of these stimuli, ensuring that the temperature in the targeted tissue remains below the necrosis threshold.
Comparison of Virtual Reality Exergames and Nature Videos on Attentional Performance: A Single-Session Study
Authors: Rodríguez-Rodríguez Elena , Castillo-Escamilla Joaquín , Nieto-Escamez Francisco
Published: September 2024

Background/Objectives: This study aimed to investigate the acute effects of a single session of a VR exergame (Beat Saber) and a VR nature video (Ireland 4K) on attentional performance, using the Flanker and Attentional Blink (AB) tasks. The objective was to assess whether these VR interventions could enhance attentional control, as measured by improvements in response times and accuracy. Methods: A total of 39 psychology students, aged 19–25, were randomly assigned to one of three groups: VR exergame, VR nature video, or control. Participants completed the Flanker and AB tasks before and after the intervention. A repeated measures design was employed to analyze changes in response times and accuracy across pre- and post-test sessions. Results: The study revealed significant improvements in response times and accuracy across all groups in the post-test measures, indicating a strong training effect. In the AB task, shorter stimulus onset asynchrony (SOA) led to decreased accuracy and slower response times, emphasizing the difficulty in processing closely spaced targets. The interaction between Type and Group in response times for target stimuli suggested that the intervention types differentially influenced processing speed in specific conditions. Conclusions: The findings suggest that while brief VR interventions did not produce significant differences between groups, the training effect observed highlights the influence of task-specific factors such as SOA and target presence. Further research is needed to explore whether longer or repeated VR sessions, as well as the optimization of task-specific parameters, might lead to more pronounced cognitive benefits.
White Matter Magnetic Resonance Diffusion Measures in Multiple Sclerosis with Overactive Bladder
Authors: Yang Xixi , Liechti Martina D. , Kanber Baris , Sudre Carole H. , Castellazzi Gloria , Zhang Jiaying , Yiannakas Marios C. , Gonzales Gwen , Prados Ferran , Toosy Ahmed T. , Gandini Wheeler-Kingshott Claudia A. M. , Panicker Jalesh N.
Published: September 2024

Background: Lower urinary tract (LUT) symptoms are reported in more than 80% of patients with multiple sclerosis (MS), most commonly an overactive bladder (OAB). The relationship between brain white matter (WM) changes in MS and OAB symptoms is poorly understood. Objectives: We aim to evaluate (i) microstructural WM differences across MS patients (pwMS) with OAB symptoms, patients without LUT symptoms, and healthy subjects using diffusion tensor imaging (DTI), and (ii) associations between clinical OAB symptom scores and DTI indices. Methods: Twenty-nine female pwMS [mean age (SD) 43.3 years (9.4)], including seventeen with OAB [mean age (SD) 46.1 years (8.6)] and nine without LUT symptoms [mean age (SD) 37.5 years (8.9)], and fourteen healthy controls (HCs) [mean age (SD) 48.5 years (20)] were scanned in a 3T MRI with a DTI protocol. Additionally, clinical scans were performed for WM lesion segmentation. Group differences in fractional anisotropy (FA) were evaluated using tract-based spatial statistics. The Urinary Symptom Profile questionnaire assessed OAB severity. Results: A statistically significant reduction in FA (p = 0.004) was identified in microstructural WM in pwMS, compared with HCs. An inverse correlation was found between FA in frontal and parietal WM lobes and OAB scores (p = 0.021) in pwMS. Areas of lower FA, although this did not reach statistical significance, were found in both frontal lobes and the rest of the non-dominant hemisphere in pwMS with OAB compared with pwMS without LUT symptoms (p = 0.072). Conclusions: This study identified that lesions affecting different WM tracts in MS can result in OAB symptoms and demonstrated the role of the WM in the neural control of LUT functions. By using DTI, the association between OAB symptom severity and WM changes were identified, adding knowledge to the current LUT working model. As MS is predominantly a WM disease, these findings suggest that regional WM involvement, including of the anterior corona radiata, anterior thalamic radiation, superior longitudinal fasciculus, and superior frontal-occipital fasciculus and a non-dominant prevalence in WM, can result in OAB symptoms. OAB symptoms in MS correlate with anisotropy changes in different white matter tracts as demonstrated by DTI. Structural impairment in WM tracts plays an important role in LUT symptoms in MS.
Fluid–Structure Interaction Simulations of the Initiation Process of Cerebral Aneurysms
Authors: Nagy Jozsef , Fenz Wolfgang , Miron Veronika M. , Thumfart Stefan , Maier Julia , Major Zoltan , Stefanits Harald , Oberndorfer Johannes , Stroh Nico , Mazanec Vanessa , Rauch Philip-Rudolf , Gruber Andreas , Gmeiner Matthias
Published: September 2024

Background: Hemodynamics during the growth process of cerebral aneurysms are incompletely understood. We developed a novel fluid–structure interaction analysis method for the identification of relevant scenarios of aneurysm onset. Method: This method integrates both fluid dynamics and structural mechanics, as well as their mutual interaction, for a comprehensive analysis. Patients with a single unruptured cerebral aneurysm were included. Results: Overall, three scenarios were identified. In scenario A, wall shear stress (WSS) was low, and the oscillatory shear index (OSI) was high in large areas within the region of aneurysm onset (RAO). In scenario B, the quantities indicated a reversed behavior, where WSS was high and OSI was low. In the last scenario C, a behavior in-between was found, with scenarios A and B coexisting simultaneously in the RAO. Structural mechanics demonstrated a similar but independent trend. Further, we analyzed the change in hemodynamics between the onset and a fully developed aneurysm. While scenarios A and C remained unchanged during aneurysm growth, 47% of aneurysms in scenario B changed into scenario A and 20% into scenario C. Conclusions: In conclusion, these findings suggest that WSS and the OSI are reciprocally regulated, and both low and high WSS/OSI conditions can lead to aneurysm onset.
Enhanced Correlation between Arousal and Infra-Slow Brain Activity in Experienced Meditators
Authors: Sihn Duho , Kim Sung-Phil
Published: September 2024

Background/Objectives: Meditation induces changes in the nervous system, which presumably underpin positive psychological and physiological effects. Such neural changes include alterations in the arousal fluctuation, as well as in infraslow brain activity (ISA, <0.1 Hz). Furthermore, it is known that fluctuations of arousal over time correlate with the oscillatory phase of ISA. However, whether this arousal–ISA correlation changes after meditation practices remains unanswered.; Methods: The present study aims to address this question by analyzing a publicly available electroencephalogram (EEG) dataset recorded during meditation sessions in the groups of experienced meditators and novices. The arousal fluctuation is measured by galvanic skin responses (GSR), and arousal–ISA correlations are measured by phase synchronization between GSR and EEG ISAs.; Results: While both groups exhibit arousal–ISA correlations, experienced meditators display higher correlations than novices. These increased arousal–ISA correlations in experienced meditators manifest more clearly when oscillatory phase differences between GSR and EEG ISAs are either 0 or π radians. As such, we further investigate the characteristics of these phase differences with respect to spatial distribution over the brain. We found that brain regions with the phase difference of either 0 or π radians form distinct spatial clusters, and that these clusters are spatially correlated with functional organization estimated by the principal gradient, based on functional connectivity.; Conclusions: Since increased arousal–ISA correlations reflect enhanced global organization of the central and autonomic nervous systems, our findings imply that the positive effects of meditation might be mediated by enhanced global organization of the nervous system.
The Big, the Dark, and the Biopsychosocial Shades of Harmony: Personality Traits and Harmony in Life
Authors: Garcia Danilo
Published: September 2024

Our current understanding of the relationship between personality traits and subjective well-being, or happiness, is limited to the conceptualization of subjective well-being as being life satisfaction and a positive affective experience (i.e., the presence of positive emotions and the absence of negative ones), thus lacking the sense of acceptance, balance, adaptation, and self-transcendent unity (i.e., harmony in life) that is appreciated as part of the good life in many ancient and modern cultures. Moreover, most studies use the Big Five Model to understand which personality traits predict subjective well-being. Here, I examine the predictive power of personality on harmony in life using the Big Five Model, the Dark Triad, and Cloninger’s Biopsychosocial Model. The present study utilized past published data from three cross-sectional studies. In each separate sample, participants self-reported personality by answering the Big Five Inventory (N1 = 297), the Short Dark Triad (N2 = 1876), or the Temperament and Character Inventory (N3 = 436). All participants (NTotal = 3698) answered to the Harmony in Life Scale. The traits in the Biopsychosocial Model explained the highest variance in harmony in life (R2 = 0.435, F(7, 428) = 47.136, p < 0.001), followed by the Big Five (R2 = 0.341, F(5, 291) = 30.110, p < 0.001) and the Dark Triad (R2 = 0.096, F(3, 1872) = 66.055, p < 0.001). The key significant predictors were Self-Directedness, Self-Transcendence, and Harm Avoidance from the Biopsychosocial Model and Agreeableness, Conscientiousness, and Neuroticism from the Big Five. Narcissism was the only predictor from the Dark Triad, although this relationship was very small. The findings underscore the importance of a multidimensional approach for understanding subjective well-being and the inclusion of harmony in life as its third component. The Biopsychosocial Model’s inclusion of both temperament and character dimensions provided the most comprehensive understanding of harmony in life. While positive traits like Agreeableness, Self-Directedness, and Self-Transcendence enhance harmony, negative traits like Neuroticism and Harm Avoidance diminish it. Moreover, research only including “dark traits” might give the impression that an inflated sense of self-importance, a deep need for admiration, and a lack of empathy for others (i.e., Narcissism) is predictive of balance in life. However, this association was not only extremely low but can be interpreted as misguided since the results using the other models showed that helpful, empathetic, kind, and self-transcendent behavior predicted harmony. These results suggest that interventions aimed at enhancing well-being should consider a broad range of personality traits, especially those that are not present in the Big Five Model, thus advocating for a biopsychosocial approach to well-being interventions.
Global Research Trends and Future Directions in Urban Historical Heritage Area Conservation and Development: A 25-Year Bibliometric Analysis
Authors: Xia Jun , Kang Jing , Xu Xiaolin
Published: September 2024

Urban historical heritage areas serve as vital repositories of urban culture and history, playing a crucial role in cultural inheritance and the promotion of urban development. The protection and development of these heritage areas are essential for preserving the cultural characteristics and architectural styles of cities. Despite the growing body of research, a comprehensive review of the dynamic evolution, research frontiers, and future trajectories in this field remains absent. To bridge this gap, this study draws on the Web of Science Core Collection database, selecting 828 papers published between 2000 and 2024 that focus on urban historical heritage conservation and development. By employing Python programming and network analysis tools, this study conducted a systematic analysis of research structures and trends over the past 25 years. The results indicate that countries such as China and Italy, along with their respective research institutions, are at the forefront of global research in this area. Furthermore, this study identified research hotspots, including historic districts, sustainable urban development, urban regeneration, risk assessment, 3D modeling, digital documentation, and cultural tourism. This research not only discusses the challenges faced in the field but also explores future development trends, providing new theoretical perspectives and practical guidance for subsequent studies.
Research on the Visual Design Elements of the Living Street from the Perspective of Human Factors Engineering
Authors: Liu Yutong , Fu Yao
Published: September 2024

Living streets are places of high activity frequency in people’s daily lives, so it is particularly important to design the street space based on people’s perceived comfort. There is a paucity of quantitative studies conducted on street interface elements, as evidenced by an examination of existing studies. Accordingly, this study used a human factor experiment to ascertain the quantitative value of interface elements that engender a sense of visual comfort. This study used a simulation experiment of a life street scene, integrating wearable physiological sensors and a subjective evaluation scale, to analyze the impact of varying scene element values on participants’ perception. The findings indicate that distinct values of street interface elements exert markedly disparate effects on people’s perception. The interface transparency that elicits a more favorable response is approximately 40%, the store density is approximately 15, and individuals demonstrate a discernible inclination towards the street scene with warm colors and rich textures.
The Seismic Behavior of a Base-Isolated Building with Simultaneous Translational and Rotational Motions during an Earthquake
Authors: Bratu Polidor , Dobre Daniela , Vasile Ovidiu , Dobrescu Cornelia-Florentina
Published: September 2024

This technical study on strengthening a five-story building in Bucharest, a city known for its high seismic activity in Europe, required implementing a base-isolation system as part of the rehabilitation solution. The main challenge was assembling the elastomeric equipment system at the base of the building and the structural system, which was considered a rigid solid with six degrees of dynamic freedom. This required defining and solving differential equations of motion for earthquake action. It was determined that switching from a six-DoF system to a three-DoF system and then customizing the results for one DoF was justified. The analysis involved designing an isolation system with elastomeric anti-seismic devices and using a dynamic model with degrees of freedom to calculate the response for the first mode (fundamental) of seismic action from the spectral composition of an earthquake. The variation in amplitudes in the three zones—pre-resonance, at resonance and after resonance—is of great interest from a practical and design perspective. Also, the support solution was optimized in terms of the isolators’ location and the stiffness and damping parameters so that the degree of dynamic isolation could be achieved at the highest possible values (I ≥ 60%).
Experimental Research on the Seismic Ductility Performance of Wavy Web PEC Beams
Authors: Yang Kejia , Lu Tianyu , Li Jie , Lou Hanzhong
Published: September 2024

To improve the out-of-plane stability of partially encased composite (PEC) beam webs and enhance the synergy between concrete and section steel, a new type of wavy web PEC beam was designed and fabricated. In this study, the flange thickness and shear–span ratio were varied as key parameters. Low-cycle reversed loading tests were conducted to investigate the effects of these variables on the load-bearing capacity, failure patterns, deformation capacity, hysteretic energy dissipation capacity, and stiffness degradation of the wavy web PEC beams. Numerical simulations were performed using ABAQUS CAE2023, a finite element analysis (FEA) software, under low-cycle reversed loading conditions. The applicability of the ABAQUS software CAE2023 for the corrugated web PEC beam model was validated by comparing test results with finite element analysis results. A detailed parametric analysis was then carried out using the finite element model to further investigate the mechanical properties of the wavy web PEC beams. The research findings are as follows: the wavy web PEC beams exhibited good ductility; a larger shear–span ratio led to a transition in the failure pattern from shear failure to flexural failure; varying the flange thickness significantly affected the failure location and characteristics; and reducing the flange thickness could limit the propagation of concrete cracks, thereby improving toughness and energy dissipation capacity.
Mechanical Properties and Stress–Strain Constitutive Relations of Coal-Fired Slag Concrete
Authors: Zhang Jianpeng , Li Gang , Yu Daidong , Lei Yingdong , Zhang Yonghua
Published: September 2024

In this study, we conducted a single-factor experiment where fine aggregates in each mixture were replaced with coal-fired slag at replacement rates in the range of 0–100%. We investigated the effect of slag substitution rate on the cubic compressive strength, splitting tensile strength, axial compressive strength, and static modulus of elasticity of slag concrete. Based on the experimental data, the stress–strain curve of the coal-fired slag concrete was divided into four phases: elastic, elasto-plastic, peak, and decline phases. A stress–strain constitutive equation was established to describe the coal-fired slag concrete. A replacement rate of 50% of the formulated coal-fired slag concrete meets the strength requirements of C60 structural applications, and the cubic compressive strength is the same as that of ordinary concrete. Coal-fired slag can be utilized in large quantities, improving the economic value of coal-fired slag and expanding the scope of application of slag concrete.
Efficient Daylighting: The Importance of Glazing Transmittance and Room Surface Reflectance
Authors: Escobar Isabel , Orduna-Hospital Elvira , Aporta Justiniano , Sanchez-Cano Ana
Published: September 2024

This study quantitatively analyzes the influence of the spectral characteristics, reflectance or transmittance, of different materials on the lighting of an interior space with natural and artificial light. For this purpose, a three-dimensional simulated classroom is used, where each of the components is assigned specific materials with an associated reflectance or transmittance. Additionally, two types of lighting are available: 6500 K daylight and light from six continuous spectrum LED luminaires. The lighting is evaluated on two planes: the work plane and the corneal plane (80 cm and 120 cm from the floor, respectively). Three versions of the same classroom were analyzed by varying the walls (white, blue, and red), each with a different neutral-colored floor. Furthermore, calculations were performed in each situation considering two different types of glazing in the windows, with 20% and 88% transmittance. The photopic and melanopic lighting analysis was carried out with the ALFA calculation program to verify the necessary requirements for adequate lighting. The results show that the white classroom is the best lit, followed by the blue and finally the red, due to the reflectance characteristics of the walls and floor although slight differences among them are found. It was found that in some cases, additional auxiliary luminaires would be required for proper lighting depending on the transmittance of the glazing. This study highlights the critical role of material selection in optimizing both photopic and melanopic lighting, with practical implications for energy efficiency and occupant well-being in educational spaces.
Upgrading of Rice Straw Bio-Oil Using 1-Butanol over ZrO2-Fe3O4 Bimetallic Nanocatalyst Supported on Activated Rice Straw Biochar to Butyl Esters
Authors: Ibrahim Alhassan , Elsayed Islam , Hassan El Barbary
Published: September 2024

Bio-oil produced via fast pyrolysis, irrespective of the biomass source, faces several limitations, such as high water content, significant oxygenated compound concentration (35–40 wt.%), a low heating value (13–20 MJ/kg), and poor miscibility with fossil fuels. These inherent drawbacks hinder the bio-oil’s desirable properties and usability, highlighting the necessity for advanced processing techniques to overcome these challenges and improve the bio-oil’s overall quality and applicability in energy and industrial sectors. To address the limitations of bio-oil, a magnetic bimetallic oxide catalyst supported on activated rice straw biochar (ZrO2-Fe3O4/AcB), which has not been previously employed for this purpose, was developed and characterized for upgrading rice straw bio-oil in supercritical butanol via esterification. Furthermore, the silica in the biochar, combined with the Lewis acid sites provided by ZrO2 and Fe3O4, offers Brønsted acid sites. This synergistic combination enhances the bio-oil’s quality by facilitating esterification, deoxygenation, and mild hydrogenation, thereby reducing oxygen content and increasing carbon and hydrogen levels. The effects of variables, including time, temperature, and catalyst load, were optimized using response surface methodology (RSM). The optimal reaction conditions were determined using a three-factor, one-response, and three-level Box-Behnken design (BBD). The ANOVA results at a 95% confidence level indicate that the results are statistically significant due to a high Fisher’s test (F-value = 37.07) and a low probability (p-value = 0.001). The minimal difference between the predicted R² and adjusted R² for the ester yield (0.0092) suggests a better fit. The results confirm that the optimal reaction conditions are a catalyst concentration of 1.8 g, a reaction time of 2 h, and a reaction temperature of 300 °C. Additionally, the catalyst can be easily recycled for four reaction cycles. Moreover, the catalyst demonstrated remarkable reusability, maintaining its activity through four consecutive reaction cycles. Its magnetic properties allow for easy separation from the reaction mixture using an external magnet.
Microbiological Bioreduction of Bulky–Bulky Pyrimidine Derivatives as an Alternative to Asymmetric Chemical Synthesis
Authors: Kołodziejska Renata , Pawluk Hanna , Tafelska-Kaczmarek Agnieszka , Baumgart Szymon , Studzińska Renata , Kosinska Agnieszka , Kwit Marcin
Published: September 2024

Heterocyclic scaffolds are often present in many natural and non-natural products with important biological activity, such as synthetic intermediates used to synthesise many drugs. Among others, heterocycles based on a pyrimidine ring may have antioxidant, antibacterial, antiviral, antifungal, antituberculosis, and anti-inflammatory properties. The present study investigated commercially available microbial biocatalysts in the enzymatic desymmetrization reaction of bulky–bulky ketones derived from pyrimidine bases. The influence of some parameters on the efficiency of biocatalysis, i.e., the substrate concentration and pH of the reaction medium, was evaluated. In the one-step bioreduction catalysed by Saccharomyces cerevisiae, secondary alcohols with a defined absolute configuration were obtained with high enantiomeric excess up to 99% ee and moderate conversion. Biocatalysis offers economic and environmental benefits as an alternative to conventional methods, becoming a powerful tool in the synthesis of crowded alcohols.
Functional Analysis of Human GBA1 Missense Mutations in Drosophila: Insights into Gaucher Disease Pathogenesis and Phenotypic Consequences
Authors: Kuppuramalingam Aparna , Cabasso Or , Horowitz Mia
Published: September 2024

The human GBA1 gene encodes lysosomal acid β-glucocerebrosidase, whose activity is deficient in Gaucher disease (GD). In Drosophila, there are two GBA1 orthologs, Gba1a and Gba1b, and Gba1b is the bona fide GCase encoding gene. Several fly lines with different deletions in the Gba1b were studied in the past. However, since most GD-associated GBA1 mutations are point mutations, we created missense mutations homologous to the two most common GD mutations: the mild N370S mutation (D415S in Drosophila) and the severe L444P mutation (L494P in Drosophila), using the CRISPR-Cas9 technology. Flies homozygous for the D415S mutation (dubbed D370S hereafter) presented low GCase activity and substrate accumulation, which led to lysosomal defects, activation of the Unfolded Protein Response (UPR), inflammation/neuroinflammation, and neurodegeneration along with earlier death compared to control flies. Surprisingly, the L494P (called L444P hereafter) flies presented higher GCase activity with fewer lysosomal defects and milder disease in comparison to that presented by the D370S homozygous flies. Treatment with ambroxol had a limited effect on all homozygous fly lines tested. Overall, our results underscore the differences between the fly and human GCase enzymes, as evidenced by the distinct phenotypic outcomes of mutations in flies compared to those observed in human GD patients.
Hardening of Mortars from Blended Cement with Opoka Additive in CO2 Environment
Authors: Siauciunas Raimundas , Prichockiene Edita , Valancius Zenonas , Elsteris Arunas
Published: September 2024

The influence of the parameters of accelerated carbonization in a 99.9% CO2 environment on the hardening kinetics of blended cement with 15 wt% opoka additive, the physical and mechanical properties of the resulting products, the mineralogical composition, and the amount of absorbed CO2 were investigated. Sedimentary rock opoka was found to have opal silica and calcite as its predominant constituent parts. Therefore, these properties determine that it serves as an extremely suitable raw material and a source of both SiO2 and CaO. The strength properties of the mortars (blended cement/standard sand = 1:3) were similar or even better than those of samples based on Ordinary Portland cement (OPC): the compressive strength exceeded 50 MPa under optimal conditions. In blended cement, some of the pores are filled with fine-dispersed opoka, which can lead to an increase in strength. By reducing the amount of OPC in mixtures, the negative impact of its production on the environment is reduced accordingly. Using XRD, DSC, and TG methods, it was determined that replacing 15 wt% of OPC clinker with opoka does not affect the mineralogy of the crystalline phases as the same compounds are obtained. After determining the optimal parameters for sample preparation and hardening, in accordance with the obtained numbers, concrete pavers of industrial dimensions (100 × 100 × 50 mm) were produced. Their strength indicators were even ~10% better.
Diverse Cobalt(II) and Iron(II/III) Coordination Complexes/Polymers Based on 4′-Pyridyl: 2,2′;6′,2″-Terpyridine: Synthesis, Structures, Catalytic and Anticancer Activities
Authors: Cheng Shu-Yuan , Zhang Qinguo , Tang Quan , Neary Michelle C. , Zheng Shengping
Published: September 2024

The success of platinum-based chemotherapeutic drugs for clinical cancer treatments has inspired tremendous research efforts on developing new metallic anticancer agents with improved cytotoxic activity and reduced side effects. 2,2′;6′,2″-Terpyridine and its 4′-substituted derivatives have showed great potential as ligand compartments for designing new anticancer drug candidates involving base metals. In this work, we synthesized a series of cobalt and iron coordination compounds based on 4′-pyridyl-2,2′;6′,2″-terpyridine, including homoleptic complexes, a dinuclear bridged complex and 1- and 2-dimensional coordination polymers/networks. The polymorphism of two homoleptic CoII and FeII complexes has been described along with the structural characterization of a CoII coordination polymer and dinuclear FeIII complex by X-ray crystallography. These compounds were tested preliminarily as precatalysts for the regioselective hydrosilylation of styrene. Their cytotoxic activities against two human breast cancer cell lines (MCF-7 and MDA-MB 468) and a normal breast epithelial cell line (MCF-10A) were investigated in order to observe the best-performing drug candidates.
Preterm Infant with Generalized Arterial Calcification of Infancy Who Survived Due to Early Diagnosis and Appropriate Treatment with Bisphosphonates: A Case Report
Authors: Tanaka Masato , Kobayashi Akira , Kuwabara Haruhiro , Nirei Jun , Ozawa Junichi , Sawano Kentaro , Shibata Nao , Nagasaki Keisuke , Saitoh Akihiko
Published: September 2024

Generalized arterial calcification of infancy (GACI) is a rare disease characterized by arterial calcification. GACI is caused by a mutation in the ENPP1 or ABCC6 genes. GACI causes severe hypertension and heart failure, and approximately 50% of patients die within the first 6 months. In particular, preterm infants with GACI often die due to immature cardiac function. Bisphosphonates are effective in treating GACI; however, no standardized treatment regimen is available. We experienced a case of a preterm infant with GACI born at 30 weeks gestation. Ultrasonography showed high-intensity lesions in the arteries, and computed tomography revealed calcification of the arteries throughout the body, leading to the diagnosis of GACI. We administered intravenous pamidronate, and her cardiac contraction improved. The initial scheduled interval between drug administrations was 2 months. However, the cardiac contraction worsened 1 month after the pamidronate administration. Therefore, we decreased the dosing interval and administered a second course of pamidronate, which improved her cardiac function. We then switched to oral etidronate. To improve the morbidity and mortality rates of preterm infants with GACI, it is important to obtain an early diagnosis of GACI by investigating high-intensity lesions in the arteries and performing early administration of an appropriate type of bisphosphonate.
Marine Bioactive Molecules as Inhibitors of the Janus Kinases: A Comparative Molecular Docking and Molecular Dynamics Simulation Approach
Authors: Ahmed Emad A. , Abdelsalam Salah A.
Published: September 2024

A treasure trove of naturally occurring biomolecules can be obtained from sea living organisms to be used as potential antioxidant and anti-inflammatory agents. These bioactive molecules can target signaling molecules involved in the severity of chronic autoimmune diseases such as rheumatoid arthritis (RA). The intracellular tyrosine kinases family, Janus kinases (JAKs, includes JAK1, JAK2, and JAK3), is implicated in the pathogenesis of RA through regulating several cytokines and inflammatory processes. In the present study, we conducted molecular docking and structural analysis investigations to explore the role of a set of bioactive molecules from marine sources that can be used as JAKs’ specific inhibitors. Around 200 antioxidants and anti-inflammatory molecules out of thousands of marine molecules found at the Comprehensive Marine Natural Products Database (CMNPD) website, were used in that analysis. The details of the interacting residues were compared to the recent FDA approved inhibitors tofacitinib and baricitinib for data validation. The shortlisted critical amino acids residues of our pharmacophore-based virtual screening were LYS905, GLU957, LEU959, and ASP1003 at JAK1, GLU930 and LEU932 at JAK2, and GLU905 and CYS909 of JAK3. Interestingly, marine biomolecules such as Sargachromanol G, Isopseudopterosin E, Seco-Pseudopterosin, and CID 10071610 showed specific binding and significantly higher binding energy to JAK1 active/potential sites when being compared with the approved inhibitors. In addition, Zoanthoxanthin and Fuscoside E bind to JAK2′s critical residues, GLU930 and LEU932. Moreover, Phorbaketal and Fuscoside E appear to be potential candidates that can inhibit JAK3 activity. These results were validated using molecular dynamics simulation for the docked complexes, JAK1(6sm8)/SG, JAK2 (3jy9)/ZAX, and JAK3 (6pjc)/Fuscoside E, where stable and lower binding energy were found based on analyzing set of parameters, discussed below (videos are attached). A promising role of these marine bioactive molecules can be confirmed in prospective preclinical/clinical investigations using rheumatoid arthritis models.
The Enhancement of Regulatory T Cell Maturation and Th1/Th2 Balance through FOXP3 Expression by Lactobacillus paracasei in an Ovalbumin-Induced Allergic Skin Animal Model
Authors: Liu Chin-Feng , Chao Wen-Yu , Shih Tsung-Wei , Lee Chun-Lin , Pan Tzu-Ming
Published: September 2024

Chronic allergic skin conditions, including atopic dermatitis (AD), are characterized by pruritus, erythema, xerosis, desquamation, and inflammation, significantly impacting quality of life. Long-term steroid use, while common in treatment, carries the risk of adverse effects. Previous studies have demonstrated the potential of Lactobacillus paracasei subsp. paracasei NTU 101 (NTU 101) in alleviating AD symptoms from a preventive perspective. This study, however, focuses on exploring NTU 101’s therapeutic potential by investigating its effects on regulatory T cell (Treg) maturation and Th1/Th2 balance. The results revealed that NTU 101 administration effectively reduced serum IgE levels and inflammatory cell infiltration in the skin, leading to a significant improvement in both epidermal and dermal thickness in the AD model. Additionally, NTU 101 modulated the immune response by increasing the proportion of CD4+/IL-4+ (Th2) cells in the spleen and concurrently enhancing FOXP3 expression in CD4+/CD25+ cells, which is critical for Treg cell development. This immune modulation was further associated with a rebalancing of the Th1/Th2 ratio, achieved by increasing the proportion of CD4+/IFN-γ+ (Th1) cells. Moreover, NTU 101 influenced the proportion of CD4+IL-17+ (Th17) cells, thereby supporting neutrophil maturation and promoting allergen clearance, ultimately mitigating AD symptoms. These findings underscore the potential of NTU 101 not only in managing AD symptoms but also in modulating key immune pathways involved in the pathogenesis of the disease, offering a promising alternative or adjunct to conventional steroid therapies.
TRIM25, TRIM28 and TRIM59 and Their Protein Partners in Cancer Signaling Crosstalk: Potential Novel Therapeutic Targets for Cancer
Authors: Chiang De Chen , Yap Beow Keat
Published: September 2024

Aberrant expression of TRIM proteins has been correlated with poor prognosis and metastasis in many cancers, with many TRIM proteins acting as key oncogenic factors. TRIM proteins are actively involved in many cancer signaling pathways, such as p53, Akt, NF-κB, MAPK, TGFβ, JAK/STAT, AMPK and Wnt/β-catenin. Therefore, this review attempts to summarize how three of the most studied TRIMs in recent years (i.e., TRIM25, TRIM28 and TRIM59) are involved directly and indirectly in the crosstalk between the signaling pathways. A brief overview of the key signaling pathways involved and their general cross talking is discussed. In addition, the direct interacting protein partners of these TRIM proteins are also highlighted in this review to give a picture of the potential protein–protein interaction that can be targeted for future discovery and for the development of novel therapeutics against cancer. This includes some examples of protein partners which have been proposed to be master switches to various cancer signaling pathways.
Exploring Water-Soluble South African Tulbaghia violacea Harv Extract as a Therapeutic Approach for Triple-Negative Breast Cancer Metastasis
Authors: Alaouna Mohammed , Hull Rodney , Molefi Thulo , Khanyile Richard , Mbodi Langanani , Luvhengo Thifhelimbilu Emmanuel , Chauke-Malinga Nkhensani , Phakathi Boitumelo , Penny Clement , Dlamini Zodwa
Published: September 2024

Triple-negative breast cancer (TNBC) accounts for approximately 20% of all breast cancer cases and is characterized by a lack of estrogen, progesterone, and human epidermal growth factor 2 receptors. Current targeted medicines have been unsuccessful due to this absence of hormone receptors. This study explored the efficacy of Tulbaghia violacea, a South African medicinal plant, for the treatment of TNBC metastasis. Extracts from T. violacea leaves were prepared using water and methanol. However, only the water-soluble extract showed anti-cancer activity and the effects of this water-soluble extract on cell adhesion, invasion, and migration, and its antioxidant activity were assessed using MCF-10A and MDA-MB-231 cells. The T. violacea extract that was soluble in water effectively decreased the movement and penetration of MDA-MB-231 cells through the basement membrane in scratch and invasion tests, while enhancing their attachment to a substance resembling an extracellular matrix. The sample showed mild-to-low antioxidant activity in the antioxidant assy. Nuclear magnetic resonance spectroscopy revealed 61 chemical components in the water-soluble extract, including DDMP, 1,2,4-triazine-3,5(2H,4H)-dione, vanillin, schisandrin, taurolidine, and α-pinene, which are known to have anti-cancer properties. An in-depth examination of the transcriptome showed alterations in genes linked to angiogenesis, metastasis, and proliferation post-treatment, with reduced activity in growth receptor signaling, angiogenesis, and cancer-related pathways, such as the Wnt, Notch, and PI3K pathways. These results indicate that T. violacea may be a beneficial source of lead chemicals for the development of potential therapeutic medicines that target TNBC metastasis. Additional studies are required to identify the precise bioactive chemical components responsible for the observed anti-cancer effects.
Ductility Index for Refractory High Entropy Alloys
Authors: Temesi Ottó K. , Varga Lajos K. , Chinh Nguyen Quang , Vitos Levente
Published: September 2024

The big advantage of refractory high entropy alloys (RHEAs) is their strength at high temperatures, but their big disadvantage is their brittleness at room temperature, which prevents their machining. There is a great need to classify the alloys in terms of brittle-ductile (B-D) properties, with easily obtainable ductility indices (DIs) ready to help design these refractory alloys. Usually, the DIs are checked by representing them as a function of fraction strain, ε. The critical values of DI and ε divide the DI—ε area into four squares. In the case of a successful DI, the points representing the alloys are located in the two diagonal opposite squares, well separating the alloys with (B-D) properties. However, due to the scatter of the data, the B-D separation is not perfect, and it is difficult to establish the critical value of DI. In this paper, we solve this problem by replacing the fracture strain parameter with new DIs that scale with the old DIs. These new DIs are based on the force constant and amplitude of thermal vibration around the Debye temperature. All of them are easily available and can be calculated from tabulated data.