bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of Chlamydial DNA from Mediterranean Loggerhead Sea Turtles in Southern Italy
Authors: Pace Antonino , Vicari Nadia , Rigamonti Sara , Magnino Simone , Borrelli Luca , Dipineto Ludovico , Fioretti Alessandro , Hochscheid Sandra , Tavares Luís , Duarte Ana
Published: March 2022

Chlamydiae are obligate intracellular bacteria that include pathogens of human and veterinary importance. Several reptiles were reported to host chlamydial agents, but pathogenicity in these animals still needs clarification. Given that only one report of chlamydiosis was described in sea turtles, and that chlamydiae might also be detected in hosts without clinical signs, the current study examined asymptomatic Mediterranean loggerhead sea turtles for the presence of chlamydial DNA. Twenty loggerhead sea turtles, rehabilitated at the Marine Turtle Research Centre (Portici, Italy), were examined collecting ocular-conjunctival, oropharyngeal and nasal swabs. Samples were processed through quantitative and conventional PCR analyses to identify Chlamydiales and Chlamydiaceae, with particular attention to C. pecorum, C. pneumoniae, C. psittaci, and C. trachomatis. Although it was not possible to determine the species of chlamydiae involved, the detection of chlamydial DNA from the collected samples suggests that these microorganisms might act as opportunistic pathogens, and underlines the role of sea turtles as potential carriers. This study highlights the presence of chlamydial agents in sea turtles, and encourages further research to fully characterize these microorganisms, in order to improve the management of the health and conservation of these endangered species, and prevent potential zoonotic implications.
Reactivity of Rheumatoid Arthritis-Associated Citrulline-Dependent Antibodies to Epstein-Barr Virus Nuclear Antigen1-3
Authors: Fanelli Ilaria , Rovero Paolo , Hansen Paul Robert , Frederiksen Jette Lautrup , Houen Gunnar , Trier Nicole Hartwig
Published: March 2022

Rheumatoid arthritis (RA) is a chronic disease which causes joint inflammation and, ultimately, erosion of the underlying bone. Diagnosis of RA is based on the presence of biomarkers, such as anti-citrullinated protein antibodies (ACPA) and rheumatoid factors, along with clinical symptoms. Much evidence points to a link between the Epstein-Barr virus and RA. In this study, we analyzed ACPA reactivity to citrullinated peptides originating from Epstein-Barr nuclear antigens (EBNA1, EBNA2, and EBNA3) in order to elaborate the diagnostic potential of citrullinated EBNA peptides. Moreover, ACPA cross-reactivity to citrullinated peptides from myelin basic protein (MBP) was analyzed, as citrullinated MBP recently was described to be associated with multiple sclerosis, and some degree of sequence homology between MBP and citrullinated EBNA exists. A peptide from EBNA2, (EBNA2-A, GQGRGRWRG-Cit-GSKGRGRMH) reacted with approximately 70% of all RA sera, whereas only limited reactivity was detected to EBNA1 and EBNA3 peptides. Moreover, screening of ACPA reactivity to hybrid peptides of EBNA3-A (EPDSRDQQS-Cit-GQRRGDENRG) and EBNA2-A and peptides containing citrulline close to the N-terminal confirmed that ACPA sera contain different populations of ACPAs. No notable ACPA reactivity to MBP peptides was found, confirming that ACPAs are specific for RA, and that other factors than the presence of a central Cit-Gly motif are crucial for antibody binding. Collectively, these findings illustrate that citrullinated EBNA2 is an optimal candidate for ACPA detection, supporting current evidence that EBV is linked to RA onset.
Relationship of Physical Activity and Sleep Duration with Self-Concept, Mediterranean Diet and Problematic Videogame Use in Children: Structural Equation Analysis as a Function of Gender
Authors: González-Valero Gabriel , Nobari Hadi , Badicu Georgian , López-Gutiérrez Carlos Javier , Moreno-Rosa Guillermo , Castro-Sánchez Manuel
Published: March 2022

Engagement in physical activity and sleep duration are crucial to the adequate psychosocial and healthy development of children. Thus, the aim of this study was to develop an explanatory model of the relationships of physical activity and sleep duration with self-concept, Mediterranean diet and problematic use of videogames. Accordingly, a structural model was contrasted by means of a multi-group analysis according to gender. A non-experimental study was conducted with a descriptive, cross-sectional design for a sample of 1154 students aged 11–12 years (M = 11.41 ± 0.49). An ad hoc questionnaire was used to record sociodemographic and physical-health attributes (physical activity time and sleep). Adherence to a Mediterranean diet was assessed using the KIDMED questionnaire, self-concept was assessed using “self-concept form-5” (AF-5) and problematic videogame use was assessed using the “Video Game-Related Experiences Questionnaire”. For data analysis, a multi-group structural equation analysis was performed. The results showed that physical activity was positively associated with self-concept, while sleep duration and self-concept were negatively related to problematic videogame use. In boys there was a direct effect of self-concept on sleep duration and adherence to the Mediterranean diet, while girls did not generate such differences. Although there is a negative association between physical activity and problematic videogame use in the theoretical model and in boys, girls showed a direct association between these factors. Finally, physical activity and sleep duration were directly related in boys and indirectly related in girls.
Thermophilic Dark Fermentation of Olive Mill Wastewater in Batch Reactors: Effect of pH and Organic Loading
Authors: Tsigkou Konstantina , Sivolapenko Natalia , Kornaros Michael
Published: March 2022

In recent decades, olive oil consumption has almost tripled worldwide. Olive oil production is linked with the production of enormous amounts of olive mill wastewater, the main by-product derived from three-phase olive mills. Due to the environmental risks of olive mill wastewater disposal, the management and valorization of the specific waste stream is of great importance. This work focuses on the thermophilic dark fermentation of olive mill wastewater in batch reactors, targeting pH optimization and the organic loading effect. A series of experiments were performed, during which the organic load of the substrate remained at 40 g/L after dilution with tap water, and the pH was tested in the range of 4.5 to 7.5. The maximum yield in terms of produced hydrogen was obtained at pH 6.0, and the yields were 0.7 mol H2/mol glucose or 0.5 L H2/Lreactor. At the same conditions, a reduction of 62% of the waste’s phenols was achieved. However, concerning the effect of organic loading at the optimized pH value (6.0), a further increase in the organic load minimized the hydrogen production, and the overall process was strongly inhibited.
Tears as the Next Diagnostic Biofluid: A Comparative Study between Ocular Fluid and Blood
Authors: Ravishankar Prashanth , Daily Anna
Published: March 2022

The need to easily isolate small molecular weight proteins and genomic fragments has prompted a search for an alternative biofluid to blood that has traversed sweat, urine, saliva, and even breath. In this study, both the genomic and proteomic profiles of tears and blood are evaluated to determine the similarity and differences between the two biofluids. Both fluids were tested utilizing microarray panels for identifying proteins as well as isolation of microRNA for sequencing. As anticipated, most (118) of the proteins detected in plasma were also detected in the tear samples, with tear samples also showing 34 unique proteins that were not found in the plasma. Over 400 microRNAs were isolated in both samples with 250 microRNA fragments commonly expressed in both tears and blood. This preliminary analysis, along with simplicity of collection and processing, lends credence to further investigate tears as an alternative biofluid to blood.
Neural Networks for Directed Connectivity Estimation in Source-Reconstructed EEG Data
Authors: Faes Axel , Vantieghem Iris , Van Hulle Marc M.
Published: March 2022

Directed connectivity between brain sources identified from scalp electroencephalography (EEG) can shed light on the brain’s information flows and provide a biomarker of neurological disorders. However, as volume conductance results in scalp activity being a mix of activities originating from multiple sources, the correct interpretation of their connectivity is a formidable challenge despite source localization being applied with some success. Traditional connectivity approaches rely on statistical assumptions that usually do not hold for EEG, calling for a model-free approach. We investigated several types of Artificial Neural Networks in estimating Directed Connectivity between Reconstructed EEG Sources and assessed their accuracy with respect to several ground truths. We show that a Long Short-Term Memory neural network with Non-Uniform Embedding yields the most promising results due to its relative robustness to differing dipole locations. We conclude that certain network architectures can compete with the already established methods for brain connectivity analysis.
Advances in Food, Bioproducts and Natural Byproducts for a Sustainable Future: From Conventional to Innovative Processes
Authors: Garcia Isidoro Garcia , Simal-Gandara Jesus , Gullo Maria
Published: March 2022

The world population is expected to reach almost 10,000 million in 2050, which entails the need to focus on sustainability and its three pillars: the economy, the environment, and society [...]
Mask R-CNN with New Data Augmentation Features for Smart Detection of Retail Products
Authors: Hsia Chih-Hsien , Chang Tsung-Hsien William , Chiang Chun-Yen , Chan Hung-Tse
Published: March 2022

Human–computer interactions (HCIs) use computer technology to manage the interfaces between users and computers. Object detection systems that use convolutional neural networks (CNNs) have been repeatedly improved. Computer vision is also widely applied to multiple specialties. However, self-checkouts operating with a faster region-based convolutional neural network (faster R-CNN) image detection system still feature overlapping and cannot distinguish between the color of objects, so detection is inhibited. This study uses a mask R-CNN with data augmentation (DA) and a discrete wavelet transform (DWT) in lieu of a faster R-CNN to prevent trivial details in images from hindering feature extraction and detection for deep learning (DL). The experiment results show that the proposed algorithm allows more accurate and efficient detection of overlapping and similarly colored objects than a faster R-CNN with ResNet 101, but allows excellent resolution and real-time processing for smart retail stores.
Microbial Biopesticides against Bacterial, Fungal and Oomycete Pathogens of Tomato, Cabbage and Chickpea
Authors: Booth James , Schenk Peer M. , Mirzaee Hooman
Published: March 2022

Biological control is an environmentally friendly approach that holds promise to complement or replace chemicals to effectively protect crop plants against pests and pathogens. Environmental samples with highly diverse and competitive microbiomes that harbor antagonistic microbes with diverse modes-of-action can provide a rich source of microbial biopesticides. In the current study, bacteria isolated from rhizosphere soil and food spoilage samples were subsequently screened against various plant fungal and oomycete pathogens in growth inhibition assays. These included the new potential biocontrol bacteria Corynebacterium flavescens, Sporosarcina aquimarina and Sporosarcina saromensis with anti-fungal and antioomycete activities. Potential candidates selected by preliminary screening in plant assays were then applied to tomato, cabbage and chickpea plants to control bacterial (Pseudomonas syringae pv. tomato), fungal (Alternaria brassicicola) and oomycete (Phytophtora medicaginis) phytopathogens. Ten potential microbial biopesticides were demonstrated to be effective against these diseases, and led to significant (p < 0.05) reductions in symptoms and/or pathogen DNA compared to mock-treated diseased plants. We conclude that new and effective microbial biopesticides to control crop pathogens can be rapidly isolated from biodiverse microbiomes, where bacteria may employ these features to effectively compete against each other.
Assessment of CMIP6 Model Performance for Air Temperature in the Arid Region of Northwest China and Subregions
Authors: Liu Fang , Xu Changchun , Long Yunxia , Yin Gang , Wang Hongyu
Published: March 2022

The arid region of northwest China (ARNC) is one of the most sensitive areas to global warming. However, the performance of new Global Climate Models (GCMs) from phase 6 of the Coupled Model Intercomparison Project (CMIP6) in simulating climate in this region, especially in the subregions, is not clear yet. Based on the temperature dataset from historical runs of CMIP6, this paper analyzed and evaluated the simulation ability of 29 GCMs in reproducing the annual mean temperature (tas), annual mean maximum temperature (tasmax) and annual mean minimum temperature (tasmin) in the ARNC and subregions from 1961 to 2014. The results show that (1) the correlation coefficients (CCs) between simulation and observation time series for the mean of two model ensembles (MME for equal-weight multi-model ensemble and PME for preferred-model ensemble) are generally better than those of 29 individual GCMs, with CCs ranging from 0.38 to 0.87 (p < 0.01). (2) All the models can simulate the significant warming trend of the three temperature elements in the study area well. However, the warming magnitude simulated by most of the models (41%) is smaller than the observations except for tasmax, which is also shown in the MME. (3) The spatial pattern of the three temperature elements can be better reflected by most models. Model simulation ability for the ARNC is better compared to that of the four subregions, with a spatial CC greater than 0.7 (p < 0.01). Among the subregions, the simulation performance of the north of Xinjiang for spatial pattern is superior to that of the other regions. (4) The preferred models for each subregion are various and should be treated differently when used. Overall, the PME outperforms both the MME and the individual models; it can not only simulate the linear trend accurately but also reduce the deviation effectively.
The Energy Model of Urban Heat Island
Authors: Dudorova Nina V. , Belan Boris D.
Published: March 2022

Despite the fact that the presence of a heat island over a city was established quite a long time ago, now there is no versatile algorithm for the determination of the urban heat island intensity. The proposed models either take into account only one or several factors for the formation of an urban heat island or do not consider physical reasons for the difference in thermodynamic conditions between a city and countryside. In this regard, it is impossible to make a forecast and determine the optimal methods for reducing the urban heat island intensity for an arbitrarily chosen city in a wide range of its characteristics and climatic conditions. This paper studies the causes for the formation of an urban heat island in order to develop the quantitative model of this process through the determination of the difference in radiation fluxes of various nature between a city and countryside (background area). A new equation allowing the intensity of an urban heat island in different seasons and different times of day, as well as under various atmospheric conditions, to be calculated from meteorological parameters measured at a stationary observation station is proposed. The model has been tested through the comparison of the results of numerical simulation with direct measurements of the heat island in Tomsk with a mobile station. It is shown that the main contributors to the formation of the heat island in Tomsk are anthropogenic heat emissions (80–90% in winter, 40–50% in summer) and absorption of shortwave radiation by the urban underlying surface (5–15% in winter, 40–50% summer). The absorption of longwave radiation by the urban underlying surface, absorption by atmospheric water vapor and other constituents, and heat consumption for evaporation are insignificant. An increase in the turbulent heat flux is responsible for the outflow of 40–50% of absorbed energy in summer and 20–30% in winter.
Improvement of Enzymatic Saccharification and Ethanol Production from Rice Straw Using Recycled Ionic Liquid: The Effect of Anti-Solvent Mixture
Authors: Chuetor Santi , Panakkal Elizabeth Jayex , Ruensodsai Thanagorn , Cheenkachorn Kraipat , Kirdponpattara Suchata , Cheng Yu-Shen , Sriariyanun Malinee
Published: March 2022

One of the major concerns for utilizing ionic liquid on an industrial scale is the cost involved in the production. Despite its proven pretreatment efficiency, expenses involved in its usage hinder its utilization. A better way to tackle this limitation could be overcome by studying the recyclability of ionic liquid. The current study has applied the Box–Behnken design (BBD) to optimize the pretreatment condition of rice straw through the usage of 1-ethyl-3-methylimidazolium acetate (EMIM-Ac) as an ionic liquid. The model predicted the operation condition with 5% solid loading at 128.4 °C for 71.83 min as an optimum pretreatment condition. Under the optimized pretreatment condition, the necessity of the best anti-solvent was evaluated among water, acetone methanol, and their combinations. The study revealed that pure methanol is the suitable choice of anti-solvent, enhancing the highest sugar yield. Recyclability of EMIM-Ac coupled with anti-solvent was conducted up to five recycles following the predicted pretreatment condition. Fermentation studies evaluated the efficacy of recycled EMIM-Ac for ethanol production with 89% more ethanol production than the untreated rice straw even after five recycles. This study demonstrates the potential of recycled ionic liquid in ethanol production, thereby reducing the production cost at the industrial level.
Alpha Lipoic-Acid Potentiates Ex Vivo Expansion of Human Steady-State Peripheral Blood Hematopoietic Primitive Cells
Authors: Debeissat Christelle , Avalon Maryse , Huart Mathilde , Duchez Pascale , Rodriguez Laura , Vlaski-Lafarge Marija , Ivanovic Zoran , Brunet de la Grange Philippe
Published: March 2022

Steady state peripheral blood (SSPB) contains hematopoietic stem and progenitor cells (HSPCs) presenting characteristics of real hematopoietic stem cells, and thus represents an interesting alternative cell supply for hematopoietic cell transplantation. Development of ex vivo expansion strategies could overcome the low HSPC numbers usually rescued from SSPB. We investigated the effect of alpha lipoic acid (ALA) on ex vivo culture of SSPB CD34 positive (CD34pos) cells on primitive cell expansion, cell cycle, and oxidative metabolism as estimated by determining the ROS and GSH content. ALA increased the ex vivo expansion of total CD34pos cells and of phenotypically defined CD34pos HSPCs subpopulations that retained in vivo repopulating capacity, concomitantly to a decreased expansion of differentiating cells. ALA did not modify cell cycle progression nor the proliferation of ex vivo expanded CD34pos cells, and coherently did not affect the ROS level. On the contrary, ALA decreased the proliferation and disturbed cell cycle progression of cells reaching a differentiated status, a phenomenon that seems to be associated with a drop in ROS level. Nonetheless, ALA affected the redox status of hematopoietic primitive cells, as it reproducibly increased GSH content. In conclusion, ALA represents an interesting molecule for the improvement of ex vivo expansion strategies and further clinical application in hematopoietic cell transplantation (HCT).
Breast Cancer Subtype-Specific miRNAs: Networks, Impacts, and the Potential for Intervention
Authors: Arun Raj Pranap , Cahill Hannah F. , Marcato Paola
Published: March 2022

The regulatory and functional roles of non-coding RNAs are increasingly demonstrated as critical in cancer. Among non-coding RNAs, microRNAs (miRNAs) are the most well-studied with direct regulation of biological signals through post-transcriptional repression of mRNAs. Like the transcriptome, which varies between tissue type and disease condition, the miRNA landscape is also similarly altered and shows disease-specific changes. The importance of individual tumor-promoting or suppressing miRNAs is well documented in breast cancer; however, the implications of miRNA networks is less defined. Some evidence suggests that breast cancer subtype-specific cellular effects are influenced by distinct miRNAs and a comprehensive network of subtype-specific miRNAs and mRNAs would allow us to better understand breast cancer signaling. In this review, we discuss the altered miRNA landscape in the context of breast cancer and propose that breast cancer subtypes have distinct miRNA dysregulation. Further, given that miRNAs can be used as diagnostic and/or prognostic biomarkers, their impact as novel targets for subtype-specific therapy is also possible and suggest important implications for subtype-specific miRNAs.
Lipoprotein(a) Serum Levels Predict Pulse Wave Velocity in Subjects in Primary Prevention for Cardiovascular Disease with Large Apo(a) Isoforms: Data from the Brisighella Heart Study
Authors: Cicero Arrigo F. G. , Fogacci Federica , Derosa Giuseppe , D’Angelo Angela , Ventura Fulvio , Rizzoli Elisabetta , D’Addato Sergio , Borghi Claudio ,   on behalf of the Brisighella Heart Study Group
Published: March 2022

In the last decades, high serum levels of lipoprotein(a) (Lp(a)) have been associated with increased cardiovascular disease (CVD) risk, in particular among individuals with smaller apolipoprotein(a) (apo(a)) isoforms than those with larger sizes. The aim of our analysis was to evaluate whether Lp(a) levels could predict early vascular aging, and whether smaller apo(a) isoforms had a predictive value for vascular aging different than larger apo(a) isoforms in a cohort of subjects free from CVD. We considered the data of a subset of Brisighella Heart Study (BHS) participants free from CVD (462 men and 516 women) who were clinically evaluated during the 2012 BHS population survey. Predictors of arterial stiffness, measured as carotid-femoral pulse wave velocity (cfPWV) were estimated by the application of a step-wise linear regression model. In our cohort, there were 511 subjects with small apo(a) size and 467 subjects with large apo(a) isoforms. Subjects with larger apo(a) isoform sizes had significantly lower serum levels of Lp(a). In the BHS subpopulation sample, cfPWV was predicted by age, systolic blood pressure (SBP), serum levels of high-density lipoprotein cholesterol (HDL-C), triglycerides (TG) and sex, higher HDL-C serum levels and female sex associated with lower values of cfPWV. In subjects with smaller apo(a) isoform sizes, predictors of cfPWV were age, SBP, sex and serum levels of HDL-C, being higher HDL-C serum levels and female sex associated to lower values of cfPWV. In subjects with larger apo(a) isoform sizes, cfPWV was predicted by age, SBP, serum levels of Lp(a) and sex, with female sex associated with lower values of cfPWV. In our subpopulation sample, Lp(a) did not predict cfPWV. However, in subjects with large apo(a) isoform sizes, Lp(a) was a significant predictor of arterial stiffness.
Teaching Elaborative Reminiscing to Support Autobiographical Memory and Relationships in Residential and Community Aged Care Services
Authors: Harris Celia B. , Van Bergen Penny , Strutt Paul A. , Picard Gabrielle K. , Harris Sophia A. , Brookman Ruth , Nelson Karn
Published: March 2022

Memories of the past are critically important as we age. For older adults receiving formal care in a range of settings, reminiscing with care staff may provide frequent opportunities for recalling autobiographical memories with a supportive conversational partner. Importantly, prior research suggests that some reminiscing conversations are more supportive than others. In the developmental literature, a long tradition of sociocultural memory research has shown how children’s autobiographical memory is scaffolded and supported by parents during reminiscing, when parents use a particular kind of conversational technique, known as “elaborative reminiscing”. In the current project, we aimed to examine whether we could enhance conversations between staff and older people receiving aged care by teaching care staff about these beneficial conversational techniques and supporting them to reminisce more often with residents/clients. We also aimed to determine whether staff members’ use of elaborative reminiscing techniques was associated with autobiographical memory details recalled by residents/clients during routine conversations. We conducted a workshop with 16 staff within a residential aged care and community care setting. We followed this with a 4-week training-and-feedback period during which staff recorded their conversations with residents and clients. Staff feedback indicated successful use of the scaffolding techniques overall, and benefits as well as barriers to their use in day-to-day practice. Analysis of the conversations demonstrated that the use of particular elaborative reminiscing techniques by staff was associated with increased recall of episodic and semantic autobiographical memory details by residents/clients. Overall, findings suggest that the principles of elaborative reminiscing may apply across the lifespan, and that the benefits of elaborative reminiscing for autobiographical memory may be particularly important in times of cognitive need. Practically, training aged care staff in specific and practical conversational tools can facilitate reminiscing for people receiving aged care.
Exploring the Impact of the Obesity Paradox on Lung Cancer and Other Malignancies
Authors: Nitsche Lindsay Joyce , Mukherjee Sarbajit , Cheruvu Kareena , Krabak Cathleen , Rachala Rohit , Ratnakaram Kalyan , Sharma Priyanka , Singh Maddy , Yendamuri Sai
Published: March 2022

There is a paradoxical relationship between obesity, as measured by BMI, and many types of cancer, including non-small-cell lung cancer. Obese non-small-cell lung cancer patients have been shown to fare better than their non-obese counterparts. To analyze the multifaceted effects of obesity on oncologic outcomes, we reviewed the literature on the obesity paradox, methods to measure adiposity, the obesity-related derangements in immunology and metabolism, and the oncologic impact of confounding variables such as gender, smoking, and concomitant medications such as statins and metformin. We analyzed how these aspects may contribute to the obesity paradox and cancer outcomes with a focus on lung cancer. We concluded that the use of BMI to measure adiposity is limited and should be replaced by a method that can differentiate abdominal obesity. We also concluded that the concomitant metabolic and immunologic derangements caused by obesity contribute to the obesity paradox. Medications, gender, and smoking are additional variables that impact oncologic outcomes, and further research needs to be performed to solidify the mechanisms.
Correlation of Intraoperative 5-ALA-Induced Fluorescence Intensity and Preoperative 11C-Methionine PET Uptake in Glioma Surgery
Authors: Shimizu Kazuhide , Tamura Kaoru , Hara Shoko , Inaji Motoki , Tanaka Yoji , Kobayashi Daisuke , Sugawara Takashi , Wakimoto Hiroaki , Nariai Tadashi , Ishii Kenji , Sakuma Ichiro , Maehara Taketoshi
Published: March 2022

Background: 5-Aminolevulinic acid (5-ALA) is widely employed to assist fluorescence-guided surgery for malignant brain tumors. Positron emission tomography with 11C-methionine (MET-PET) represents the activity of brain tumors with precise boundaries but is not readily available. We hypothesized that quantitative 5-ALA-induced fluorescence intensity might correlate with MET-PET uptake in gliomas. Methods: Adult patients with supratentorial astrocytic gliomas who underwent preoperative MET-PET and surgical tumor resection using 5-ALA were enrolled in this prospective study. The regional tumor uptake of MET-PET was expressed as the ratio of standardized uptake volume max to that of the normal contralateral frontal lobe. A spectrometric fluorescence detection system measured tumor specimens’ ex vivo fluorescence intensity at 635 nm. Ki-67 index and IDH mutation status were assessed by histopathological analysis. Use of an antiepileptic drug (AED) and contrast enhancement pattern on MRI were also investigated. Results: Thirty-two patients, mostly with Glioblastoma IDH wild type (46.9%) and anaplastic astrocytoma IDH mutant (21.9%), were analyzed. When the fluorescence intensity was ranked into four groups, the strongest fluorescence group exhibited the highest mean MET-PET uptake and Ki-67 index values. When rearranged into fluorescence Visible or Non-visible groups, the Visible group had significantly higher MET-PET uptake and Ki-67 index compared to the Non-visible group. Contrast enhancement on MRI and IDH wild type tumors were more frequent among the Visible group. AED use did not correlate with 5-ALA-induced fluorescence intensity. Conclusions: In astrocytic glioma surgery, visible 5-ALA-induced fluorescence correlated with high MET-PET uptake, along with a high Ki-67 index.
A Novel Blood-Based microRNA Diagnostic Model with High Accuracy for Multi-Cancer Early Detection
Authors: Zhang Andrew , Hu Hai
Published: March 2022

Early detection is critical to reduce cancer deaths as treating early stage cancers is more likely to be successful. However, patients with early stage diseases are often asymptomatic and thus less likely to be diagnosed. Here, we utilized four microarray datasets with a standardized platform to investigate comprehensive microRNA expression profiles from 7536 serum samples. A 4-miRNA diagnostic model was developed from the lung cancer training set (n = 416, 208 lung cancer patients and 208 non-cancer participants). The model showed 99% sensitivity and specificity in the lung cancer validation set (n = 3328, 1358 cancer patients and 1970 non-cancer participants); and the sensitivity remained to be >99% for patients with stage 1 disease. When applied to the additional combined dataset of 3792 participants including 2038 cancer patients across 12 different cancer types and 1754 independent non-cancer controls, the model demonstrated high sensitivities ranging from 83.2 to 100% for biliary tract, bladder, colorectal, esophageal, gastric, glioma, liver, pancreatic, and prostate cancers, and showed reasonable sensitivities of 68.2 and 72.0% for ovarian cancer and sarcoma, respectively, while maintaining 99.3% specificity. Our study provided a proof-of-concept data in demonstrating that the 4-miRNA model has the potential to be developed into a simple, inexpensive and noninvasive blood test for early detection of multiple cancers with high accuracy.
Mitochondrial DNA on Tumor-Associated Macrophages Polarization and Immunity
Authors: Guo Yaxin , Tsai Hsiang-i , Zhang Lirong , Zhu Haitao
Published: March 2022

As the richest immune cells in most tumor microenvironments (TMEs), tumor-associated macrophages (TAMs) play an important role in tumor development and treatment sensitivity. The phenotypes and functions of TAMs vary according to their sources and tumor progression. Different TAM phenotypes display distinct behaviors in terms of tumor immunity and are regulated by intracellular and exogenous molecules. Additionally, dysfunctional and oxidatively stressed mitochondrial-derived mitochondrial DNA (mtDNA) plays an important role in remodeling the phenotypes and functions of TAMs. This article reviews the interactions between mtDNA and TAMs in the TME and further discusses the influence of their performance on tumor genesis and development.
Radiolabeled Antibodies for Cancer Imaging and Therapy
Authors: Parakh Sagun , Lee Sze Ting , Gan Hui K. , Scott Andrew M.
Published: March 2022

Radioimmunoconjugates consist of a monoclonal antibody (mAb) linked to a radionuclide. Radioimmunoconjugates as theranostics tools have been in development with success, particularly in hematological malignancies, leading to approval by the US Food and Drug Administration (FDA) for the treatment of non-Hodgkin’s lymphoma. Radioimmunotherapy (RIT) allows for reduced toxicity compared to conventional radiation therapy and enhances the efficacy of mAbs. In addition, using radiolabeled mAbs with imaging methods provides critical information on the pharmacokinetics and pharmacodynamics of therapeutic agents with direct relevance to the optimization of the dose and dosing schedule, real-time antigen quantitation, antigen heterogeneity, and dynamic antigen changes. All of these parameters are critical in predicting treatment responses and identifying patients who are most likely to benefit from treatment. Historically, RITs have been less effective in solid tumors; however, several strategies are being investigated to improve their therapeutic index, including targeting patients with minimal disease burden; using pre-targeting strategies, newer radionuclides, and improved labeling techniques; and using combined modalities and locoregional application. This review provides an overview of the radiolabeled intact antibodies currently in clinical use and those in development.
A Review on Biological Synthesis of the Biodegradable Polymers Polyhydroxyalkanoates and the Development of Multiple Applications
Authors: Dalton Bryan , Bhagabati Purabi , De Micco Jessica , Padamati Ramesh Babu , O’Connor Kevin
Published: March 2022

Polyhydroxyalkanoates, or PHAs, belong to a class of biopolyesters where the biodegradable PHA polymer is accumulated by microorganisms as intracellular granules known as carbonosomes. Microorganisms can accumulate PHA using a wide variety of substrates under specific inorganic nutrient limiting conditions, with many of the carbon-containing substrates coming from waste or low-value sources. PHAs are universally thermoplastic, with PHB and PHB copolymers having similar characteristics to conventional fossil-based polymers such as polypropylene. PHA properties are dependent on the composition of its monomers, meaning PHAs can have a diverse range of properties and, thus, functionalities within this biopolyester family. This diversity in functionality results in a wide array of applications in sectors such as food-packaging and biomedical industries. In order for PHAs to compete with the conventional plastic industry in terms of applications and economics, the scale of PHA production needs to grow from its current low base. Similar to all new polymers, PHAs need continuous technological developments in their production and material science developments to grow their market opportunities. The setup of end-of-life management (biodegradability, recyclability) system infrastructure is also critical to ensure that PHA and other biobased biodegradable polymers can be marketed with maximum benefits to society. The biobased nature and the biodegradability of PHAs mean they can be a key polymer in the materials sector of the future. The worldwide scale of plastic waste pollution demands a reformation of the current polymer industry, or humankind will face the consequences of having plastic in every step of the food chain and beyond. This review will discuss the aforementioned points in more detail, hoping to provide information that sheds light on how PHAs can be polymers of the future.
Photocatalytic Reduction of Nitrates and Combined Photodegradation with Ammonium
Authors: Conte Francesco , Pellegatta Veronica , Di Michele Alessandro , Ramis Gianguido , Rossetti Ilenia
Published: March 2022

Bare titania and metal-promoted TiO2 catalysts were employed in the treatment of nitrates, which are ubiquitous pollutants of wastewater. The results show that the process can be carried out under visible light (from a white light LED lamp) and, in the best case, 23.5% conversion of nitrate was obtained over 4 h with full selectivity towards N2 by employing 0.1 mol% Ag/TiO2 prepared by flame spray pyrolysis. Moreover, the performance was worse when testing the same catalysts with tap water (11.3% conversion), due to the more complex composition of the matrix. Finally, it was found that photoreduction of nitrate can be effectively performed in combination with photo-oxidation of ammonium without loss in the activity, opening up the possibility of treating highly polluted wastewater with a single process. The latter treatment employs the two contaminants simultaneously as electron and holes scavengers, with very good selectivity, in a completely new process that we may call Photo-Selective Catalytic Reduction (Photo-SCR).
(±)-Catechin—A Mass-Spectrometry-Based Exploration Coordination Complex Formation with FeII and FeIII
Authors: Kubicova Lenka , Bachmann Gert , Weckwerth Wolfram , Chobot Vladimir
Published: March 2022

Catechin is an extensively investigated plant flavan-3-ol with a beneficial impact on human health that is often associated with antioxidant activities and iron coordination complex formation. The aim of this study was to explore these properties with FeII and FeIII using a combination of nanoelectrospray-mass spectrometry, differential pulse voltammetry, site-specific deoxyribose degradation assay, FeII autoxidation assay, and brine shrimp mortality assay. Catechin primarily favored coordination complex formation with Fe ions of the stoichiometry catechin:Fe in the ratio of 1:1 or 2:1. In the detected Fe–catechin coordination complexes, FeII prevailed. Differential pulse voltammetry, the site-specific deoxyribose degradation, and FeII autoxidation assays proved that coordination complex formation affected catechin’s antioxidant effects. In situ formed Fe–catechin coordination complexes showed no toxic activities in the brine shrimp mortality assay. In summary, catechin has properties for the possible treatment of pathological processes associated with ageing and degeneration, such as Alzheimer’s and Parkinson’s diseases.
Oxidative Stress and AKT-Associated Angiogenesis in a Zebrafish Model and Its Potential Application for Withanolides
Authors: Tang Jen-Yang , Cheng Yuan-Bin , Chuang Ya-Ting , Yang Kun-Han , Chang Fang-Rong , Liu Wangta , Chang Hsueh-Wei
Published: March 2022

Oxidative stress and the AKT serine/threonine kinase (AKT) signaling pathway are essential regulators in cellular migration, metastasis, and angiogenesis. More than 300 withanolides were discovered from the plant family Solanaceae, exhibiting diverse functions. Notably, the relationship between oxidative stress, AKT signaling, and angiogenesis in withanolide treatments lacks comprehensive understanding. Here, we summarize connecting evidence related to oxidative stress, AKT signaling, and angiogenesis in the zebrafish model. A convenient vertebrate model monitored the in vivo effects of developmental and tumor xenograft angiogenesis using zebrafish embryos. The oxidative stress and AKT-signaling-modulating abilities of withanolides were highlighted in cancer treatments, which indicated that further assessments of their angiogenesis-modulating potential are necessary in the future. Moreover, targeting AKT for inhibiting AKT and its AKT signaling shows the potential for anti-migration and anti-angiogenesis purposes for future application to withanolides. This particularly holds for investigating the anti-angiogenetic effects mediated by the oxidative stress and AKT signaling pathways in withanolide-based cancer therapy in the future.
Methodological Flaws in Meta-Analyses of Clinical Studies on the Management of Knee Osteoarthritis with Stem Cells: A Systematic Review
Authors: Schmitz Christoph , Alt Christopher , Pearce David A. , Furia John P. , Maffulli Nicola , Alt Eckhard U.
Published: March 2022

(1) Background: Conclusions of meta-analyses of clinical studies may substantially influence opinions of prospective patients and stakeholders in healthcare. Nineteen meta-analyses of clinical studies on the management of primary knee osteoarthritis (pkOA) with stem cells, published between January 2020 and July 2021, came to inconsistent conclusions regarding the efficacy of this treatment modality. It is possible that a separate meta-analysis based on an independent, systematic assessment of clinical studies on the management of pkOA with stem cells may reach a different conclusion. (2) Methods: PubMed, Web of Science, and the Cochrane Library were systematically searched for clinical studies and meta-analyses of clinical studies on the management of pkOA with stem cells. All clinical studies and meta-analyses identified were evaluated in detail, as were all sub-analyses included in the meta-analyses. (3) Results: The inconsistent conclusions regarding the efficacy of treating pkOA with stem cells in the 19 assessed meta-analyses were most probably based on substantial differences in literature search strategies among different authors, misconceptions about meta-analyses themselves, and misconceptions about the comparability of different types of stem cells with regard to their safety and regenerative potential. An independent, systematic review of the literature yielded a total of 183 studies, of which 33 were randomized clinical trials, including a total of 6860 patients with pkOA. However, it was not possible to perform a scientifically sound meta-analysis. (4) Conclusions: Clinicians should interpret the results of the 19 assessed meta-analyses of clinical studies on the management of pkOA with stem cells with caution and should be cautious of the conclusions drawn therein. Clinicians and researchers should strive to participate in FDA and/or EMA reviewed and approved clinical trials to provide clinically and statistically valid efficacy.
Single Cell Effects of Photobiomodulation on Mitochondrial Membrane Potential and Reactive Oxygen Species Production in Human Adipose Mesenchymal Stem Cells
Authors: Pan Li-Chern , Hang Nguyen-Le-Thanh , Colley Mamadi M.S , Chang Jungshan , Hsiao Yu-Cheng , Lu Long-Sheng , Li Bing-Sian , Chang Cheng-Jen , Yang Tzu-Sen
Published: March 2022

Photobiomodulation (PBM) has recently emerged in cellular therapy as a potent alternative in promoting cell proliferation, migration, and differentiation during tissue regeneration. Herein, a single-cell near-infrared (NIR) laser irradiation system (830 nm) and the image-based approaches were proposed for the investigation of the modulatory effects in mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS), and vesicle transport in single living human adipose mesenchymal stem cells (hADSCs). The irradiated-hADSCs were then stained with 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) and Rhodamine 123 (Rh123) to represent the ΔΨm and ROS production, respectively, with irradiation in the range of 2.5–10 (J/cm2), where time series of bright-field images were obtained to determine the vesicle transport phenomena. Present results showed that a fluence of 5 J/cm2 of PBM significantly enhanced the ΔΨm, ROS, and vesicle transport phenomena compared to the control group (0 J/cm2) after 30 min PBM treatment. These findings demonstrate the efficacy and use of PBM in regulating ΔΨm, ROS, and vesicle transport, which have potential in cell proliferation, migration, and differentiation in cell-based therapy.
From Philanthropy to Clinical Care through Research: Impact of the Norman Saunders Complex Care Initiative
Authors: Macarthur Colin , Cohen Eyal , Adams Sherri , Buchanan Francine , Saunders Natasha R. , Friedman Jeremy N.
Published: March 2022

Norman Saunders was a respected academic community paediatrician who was passionate about the care of children with medical complexity. Following his untimely death at age 60, patients, friends, and colleagues raised funds to create the Norman Saunders Complex Care Initiative (NSCCI). Dr. Saunders’s vision was a comprehensive, coordinated, and integrated clinical program for children with medical complexity that was informed by research evidence. The objective of this review was to evaluate the impact of targeted philanthropic funding on research, clinical care, and policy. Since 2006, NSCCI funds have been used to support interdisciplinary and innovative research. Funded projects have reflected a breadth of research questions (clinical care, training, health system delivery, social determinants), disciplines, and methods, and the research results have informed and helped build an internationally renowned clinical program in complex care. Philanthropic funding was the foundation for the NSCCI, which over the last 15 years has built research and clinical capacity, catalysed clinical and research networks, helped train paediatric residents, influenced policy, and improved the health and well-being of children with medical complexity and their families across Canada, and beyond.
Cyclic Oxidation Properties of the Nanocrystalline AlCrFeCoNi High-Entropy Alloy Coatings Applied by the Atmospheric Plasma Spraying Technique
Authors: Ghadami Farzin , Davoudabadi Mohammad Amin , Ghadami Soheil
Published: March 2022

Microcrystalline and nanocrystalline AlCrFeCoNi high-entropy alloy (HEA) coatings were applied on Inconel 718 superalloy using the atmospheric plasma spraying (APS) process. The high-temperature oxidation behavior of the microcrystalline and nanocrystalline AlCrFeCoNi HEA-coated superalloy was examined at 1100 °C under the air atmosphere for 50 cycles under cyclic heating and cooling (1 h for each cycle). The oxidation kinetics of both nanocrystalline- and microcrystalline-coated superalloys were accordingly analyzed by weight change measurements. We noted that the uncoated and coated samples followed the parabolic rate law of the oxidation. X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy dispersive X-ray analysis (EDS), elemental mapping and X-ray photoelectron spectroscopy (XPS) were used to analyze the oxidized coated and uncoated samples. In the HEA-coated superalloy, Fe, Ni, Co and Al were oxidized in the inter-splat region, whereas the splats, which consisted mainly of Ni and Cr, remained unoxidized. Due to the formation of compact and adhesive thin NiO, CoO oxides and spinels together with the Al2O3 oxide scale on the surface of the coating during oxidation, the developed nanocrystalline HEA coating showed better oxidation resistance compared with the microcrystalline HEA coating.
Effect of Community and Socio-Economic Factors on Cardiovascular, Cancer and Cardio-Oncology Patients with COVID-19
Authors: Batra Akshee , Swaby Justin , Raval Priyanka , Zhu Haidong , Weintraub Neal Lee , Terris Martha , Karim Nagla Abdel , Keruakous Amany , Gutterman David , Beyer Kirsten , Stolley Melinda , Brown Sherry-Ann , Guha Avirup
Published: March 2022

The Coronavirus Disease 2019 (COVID-19) is a world-wide health crisis on a scale that has not been witnessed in modern times. Socio-economic (SE) factors impact every facet of human existence, including lifestyle, which significantly affects health-related quality of life. This article compiles major studies and discusses health disparities based on SE and community status in cardiovascular and cancer patients with a special focus on cardio-oncology in the context of COVID-19.