bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of the First Animal Toxin Acting as an Antagonist on AT1 Receptor
Authors: Van Baelen Anne-Cécile , Iturrioz Xavier , Chaigneau Marion , Kessler Pascal , Llorens-Cortes Catherine , Servent Denis , Gilles Nicolas , Robin Philippe
Published: January 2023

The renin-angiotensin system (RAS) is one of the main regulatory systems of cardiovascular homeostasis. It is mainly composed of angiotensin-converting enzyme (ACE) and angiotensin II receptors AT1 and AT2. ACE and AT1 are targets of choice for the treatment of hypertension, whereas the AT2 receptor is still not exploited due to the lack of knowledge of its physiological properties. Peptide toxins from venoms display multiple biological functions associated with varied chemical and structural properties. If Brazilian viper toxins have been described to inhibit ACE, no animal toxin is known to act on AT1/AT2 receptors. We screened a library of toxins on angiotensin II receptors with a radioligand competition binding assay. Functional characterization of the selected toxin was conducted by measuring second messenger production, G-protein activation and β-arrestin 2 recruitment using bioluminescence resonance energy transfer (BRET) based biosensors. We identified one original toxin, A-CTX-cMila, which is a 7-residues cyclic peptide from Conus miliaris with no homology sequence with known angiotensin peptides nor identified toxins, displaying a 100-fold selectivity for AT1 over AT2. This toxin shows a competitive antagonism mode of action on AT1, blocking Gαq, Gαi3, GαoA, β-arrestin 2 pathways and ERK1/2 activation. These results describe the first animal toxin active on angiotensin II receptors.
Association between 20% Albumin Use and Acute Kidney Injury in Major Abdominal Surgery with Transfusion
Authors: Kim Hye Jin , Kim Hyun Joo , Park Jin Ha , Shin Hye Jung , Yu Sung Kyung , Roh Yun Ho , Jeon Soo Yeon , Kim So Yeon
Published: January 2023

Red blood cell (RBC) transfusion and albumin administration can affect kidney function. We aimed to evaluate the association between intraoperative 20% albumin administration and acute kidney injury (AKI), along with the duration of hospitalization and 30-day mortality in patients undergoing major abdominal surgery with RBC transfusion. This retrospective study included 2408 patients who received transfusions during major abdominal surgery. Patients were categorized into albumin (n = 842) or no-albumin (n = 1566) groups. We applied inverse probability of treatment weighting (IPTW), propensity score (PS) matching (PSM), and PS covariate adjustment to assess the effect of albumin administration on the outcomes. In the unadjusted cohort, albumin administration was significantly associated with increased risk of AKI, prolonged hospitalization, and higher 30-day mortality. However, there was no significant association between albumin administration and AKI after adjustment (OR 1.26, 95% CI 0.90–1.76 for the IPTW; OR 1.03, 95% CI 0.72–1.48 for the PSM; and OR 1.04, 95% CI 0.76–1.43 for the PS covariate adjustment methods). While albumin exposure remained associated with prolonged hospitalization after adjustment, it did not affect 30-day mortality. Our findings suggest that hyper-oncotic albumin can be safely administered to patients who are at risk of developing AKI due to RBC transfusion.
Finerenone, a Non-Steroidal Mineralocorticoid Receptor Antagonist, Reduces Vascular Injury and Increases Regulatory T-Cells: Studies in Rodents with Diabetic and Neovascular Retinopathy
Authors: Jerome Jack R. , Deliyanti Devy , Suphapimol Varaporn , Kolkhof Peter , Wilkinson-Berka Jennifer L.
Published: January 2023

Vision loss in diabetic retinopathy features damage to the blood–retinal barrier and neovascularization, with hypertension and the renin–angiotensin system (RAS) having causal roles. We evaluated if finerenone, a non-steroidal mineralocorticoid receptor (MR) antagonist, reduced vascular pathology and inflammation in diabetic and neovascular retinopathy. Diabetic and hypertensive transgenic (mRen-2)27 rats overexpressing the RAS received the MR antagonist finerenone (10 mg/kg/day, oral gavage) or the angiotensin-converting enzyme inhibitor perindopril (10 mg/kg/day, drinking water) for 12 weeks. As retinal neovascularization does not develop in diabetic rodents, finerenone (5 mg/kg/day, i.p.) was evaluated in murine oxygen-induced retinopathy (OIR). Retinal vasculopathy was assessed by measuring gliosis, vascular leakage, neovascularization, and VEGF. Inflammation was investigated by quantitating retinal microglia/macrophages, pro-inflammatory mediators, and anti-inflammatory regulatory T-cells (Tregs). In diabetes, both treatments reduced systolic blood pressure, gliosis, vascular leakage, and microglial/macrophage density, but only finerenone lowered VEGF, ICAM-1, and IL-1ß. In OIR, finerenone reduced neovascularization, vascular leakage, and microglial density, and increased Tregs in the blood, spleen, and retina. Our findings, in the context of the FIDELIO-DKD and FIGARO-DKD trials reporting the benefits of finerenone on renal and cardiovascular outcomes in diabetic kidney disease, indicate the potential of finerenone as an effective oral treatment for diabetic retinopathy.
The Antimicrobial Activity of Curcumin and Xanthohumol on Bacterial Biofilms Developed over Dental Implant Surfaces
Authors: Alonso-Español Andrea , Bravo Enrique , Ribeiro-Vidal Honorato , Virto Leire , Herrera David , Alonso Bettina , Sanz Mariano
Published: January 2023

In search for natural products with antimicrobial properties for use in the prevention and treatment of peri-implantitis, the purpose of this investigation was to evaluate the antimicrobial activity of curcumin and xanthohumol, using an in vitro multi-species dynamic biofilm model including Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of curcumin (5 mM) and xanthohumol (100 μM) extracts, and the respective controls, were evaluated with 72-h biofilms formed over dental implants by their submersion for 60 seconds. The evaluation was assessed by quantitative polymerase chain reaction (qPCR), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). For the data analysis, comparisons were tested applying ANOVA tests with post-hoc Bonferroni corrections to evaluate the antimicrobial activity of both extracts. With qPCR, statistically significant reductions in bacterial counts were observed for curcumin and xanthohumol, when compared to the negative control. The results with CLSM and SEM were consistent with those reported with qPCR. It was concluded that both curcumin and xanthohumol have demonstrated antimicrobial activity against the six bacterial species included in the dynamic in vitro biofilm model used.
Glutamine Metabolism in Cancer Stem Cells: A Complex Liaison in the Tumor Microenvironment
Authors: Pacifico Francesco , Leonardi Antonio , Crescenzi Elvira
Published: January 2023

In this review we focus on the role of glutamine in control of cancer stem cell (CSC) fate. We first provide an overview of glutamine metabolism, and then summarize relevant studies investigating how glutamine metabolism modulates the CSC compartment, concentrating on solid tumors. We schematically describe how glutamine in CSC contributes to several metabolic pathways, such as redox metabolic pathways, ATP production, non-essential aminoacids and nucleotides biosynthesis, and ammonia production. Furthermore, we show that glutamine metabolism is a key regulator of epigenetic modifications in CSC. Finally, we briefly discuss how cancer-associated fibroblasts, adipocytes, and senescent cells in the tumor microenvironment may indirectly influence CSC fate by modulating glutamine availability. We aim to highlight the complexity of glutamine’s role in CSC, which supports our knowledge about metabolic heterogeneity within the CSC population.
sFgl2-Treg Positive Feedback Pathway Protects against Atherosclerosis
Authors: An Tianhui , Guo Mengyuan , Fan Cheng , Huang Shiyuan , Liu Hui , Liu Kun , Wang Zhaohui
Published: January 2023

Soluble fibrinogen-like protein 2 (sFgl2), a novel effector of regulatory T cells (Tregs), has been demonstrated to have potent immunosuppressive functions. Multiple studies indicate that Tregs could exert important atheroprotective effects, but their numbers gradually decrease during atherogenesis. The receptor of sFgl2 can be expressed on Treg precursor cells, while the role of sFgl2 on Treg differentiation and atherosclerosis progression remains unclear. Firstly, we detected that the sFgl2 was decreased in humans and mice with atherosclerotic diseases and was especially lower in their vulnerable plaques. Then, we used both Adeno-associated virus-sFgl2 (AAV-sFgl2)-injected ApoE-/- mice, which is systemic overexpression of sFgl2, and sFgl2TgApoE-/- bone marrow cells (BMC)-transplanted ApoE-/- mice, which is almost immune-system-specific overexpression of sFgl2, to explore the role of sFgl2 in atherosclerosis. Our experiment data showed that AAV-sFgl2 and BMT-sFgl2 could reduce atherosclerotic area and enhance plaque stability. Mechanistically, sFgl2 increases the abundance and immunosuppressive function of Tregs, which is partly mediated by binding to FcγRIIB receptors and phosphorylating Smad2/3. Collectively, sFgl2 has an atheroprotective effect that is mainly achieved by forming a positive feedback pathway with Treg. sFgl2 and Treg could synergistically protect against atherosclerosis.
Ca2+-Permeable AMPA Receptors Contribute to Changed Dorsal Horn Neuronal Firing and Inflammatory Pain
Authors: Kopach Olga , Dobropolska Yulia , Belan Pavel , Voitenko Nana
Published: January 2023

The dorsal horn (DH) neurons of the spinal cord play a critical role in nociceptive input integration and processing in the central nervous system. Engaged neuronal classes and cell-specific excitability shape nociceptive computation within the DH. The DH hyperexcitability (central sensitisation) has been considered a fundamental mechanism in mediating nociceptive hypersensitivity, with the proven role of Ca2+-permeable AMPA receptors (AMPARs). However, whether and how the DH hyperexcitability relates to changes in action potential (AP) parameters in DH neurons and if Ca2+-permeable AMPARs contribute to these changes remain unknown. We examined the cell-class heterogeneity of APs generated by DH neurons in inflammatory pain conditions to address these. Inflammatory-induced peripheral hypersensitivity increased DH neuronal excitability. We found changes in the AP threshold and amplitude but not kinetics (spike waveform) in DH neurons generating sustained or initial bursts of firing patterns. In contrast, there were no changes in AP parameters in the DH neurons displaying a single spike firing pattern. Genetic knockdown of the molecular mechanism responsible for the upregulation of Ca2+-permeable AMPARs allowed the recovery of cell-specific AP changes in peripheral inflammation. Selective inhibition of Ca2+-permeable AMPARs in the spinal cord alleviated nociceptive hypersensitivity, both thermal and mechanical modalities, in animals with peripheral inflammation. Thus, Ca2+-permeable AMPARs contribute to shaping APs in DH neurons and nociceptive hypersensitivity. This may represent a neuropathological mechanism in the DH circuits, leading to aberrant signal transfer to other nociceptive pathways.
Extracellular Vesicles as Therapeutic Resources in the Clinical Environment
Authors: Sanz-Ros Jorge , Mas-Bargues Cristina , Romero-García Nekane , Huete-Acevedo Javier , Dromant Mar , Borrás Consuelo
Published: January 2023

The native role of extracellular vesicles (EVs) in mediating the transfer of biomolecules between cells has raised the possibility to use them as therapeutic vehicles. The development of therapies based on EVs is now expanding rapidly; here we will describe the current knowledge on different key points regarding the use of EVs in a clinical setting. These points are related to cell sources of EVs, isolation, storage, and delivery methods, as well as modifications to the releasing cells for improved production of EVs. Finally, we will depict the application of EVs therapies in clinical trials, considering the impact of the COVID-19 pandemic on the development of these therapies, pointing out that although it is a promising therapy for human diseases, we are still in the initial phase of its application to patients.
The Roles of Coronary Computed Tomography Angiography in Characterizing Coronary Plaque: Screening, Treatment, and Prevention
Authors: Pour-Ghaz Issa , Alkhatib Deya , Isa Sakiru , Al-Taweel Omar , Ugonabo Ifeoma , Yedlapati Neeraja , Jefferies John Lynn
Published: January 2023

One of the major risk factors for coronary atherosclerosis is the gradual formation and maturation of coronary atherosclerotic plaque (CAP) [...]
Maxillary Antral Pseudocyst Drift after Osteotome Sinus Floor Elevation with Simultaneous Implant Placement: A Case Report and Literature Review
Authors: Wang Peihan , Huang Nan , Ren Jiayin , Gong Ping , Long Jie , Huang Bo
Published: January 2023

This report describes maxillary antral pseudocyst drift after maxillary sinus floor augmentation through osteotome sinus floor elevation with simultaneous implant placement. 3D Slicer was used to measure the pseudocyst and maxilla for the placement of the implants; follow-up visits were scheduled at 6, 12, and 22 months. No adverse effects were observed during or after surgery, and all implants exhibited osseointegration without mobility. At 6 months after surgery, the pseudocyst had moved posterolaterally from the preoperative position near the anterior medial maxillary sinus, then returned to its original position at 12 months. However, it had remigrated to the posterolateral position at 22 months. The preoperative volume of the pseudocyst was 3.795 mm3; it was 2.370, 3.439, and 2.930 mm3 at 6, 12, and 22 months after surgery, respectively. The changes in pseudocyst drift and volume did not have a substantial negative influence on the implants, presumably because of cystic attachment and the recurrence of multiple pseudocysts at different locations. The risks associated with changes in a pseudocyst can be avoided, if an appropriate treatment plan is selected.
Influence of Analytic Processing on Divergent and Convergent Thinking Tasks: The Role of Rational and Experiential Thinking Styles
Authors: Hongdizi Jianati , Cui Yu-Xin , Zhou Xiang , Zhai Hong-Kun
Published: January 2023

Scientific interest in the relationship between analytic processing and creativity has increased in recent years. However, there is conflicting evidence on whether analytic processing reduces or enhances creativity. We hypothesize that differences in creativity measurement paradigms (divergent or convergent thinking tasks) and the research orientation of analytic processing (dispositional or situational) may explain the conflicting findings. The present study aims to investigate how priming analytic processing affects individuals’ performance on divergent and convergent thinking tasks and the moderating role of thinking styles. In Study 1 (N = 155), participants were assigned to either an analytic processing group or a control group and performed convergent thinking (Remote Associates Task) and divergent thinking (Alternative Uses Test) tasks after priming. In Study 2 (N = 119), we conducted a priming paradigm of analytic processing that differed from Study 1, and a personal experiential-rational thinking style was introduced as a moderator. Results showed that priming analytic processing promoted convergent thinking performance but decreased fluency and flexibility scores on the divergent thinking task (Study 1). Notably, the effect of priming analytic processing on divergent thinking performance was significant only for participants with higher levels of rational thinking style (Study 2). These results suggest that thinking styles and dimensions of creativity should be considered in the relationship between analytic processing and creativity.
Application of Artificial Intelligence in Marine Corrosion Prediction and Detection
Authors: Imran Md Mahadi Hasan , Jamaludin Shahrizan , Ayob Ahmad Faisal Mohamad , Ali Ahmad Ali Imran Mohd , Ahmad Sayyid Zainal Abidin Syed , Akhbar Mohd Faizal Ali , Suhrab Mohammed Ismail Russtam , Zainal Nasharuddin , Norzeli Syamimi Mohd , Mohamed Saiful Bahri
Published: January 2023

One of the biggest problems the maritime industry is currently experiencing is corrosion, resulting in short and long-term damages. Early prediction and proper corrosion monitoring can reduce economic losses. Traditional approaches used in corrosion prediction and detection are time-consuming and challenging to execute in inaccessible areas. Due to these reasons, artificial intelligence-based algorithms have become the most popular tools for researchers. This study discusses state-of-the-art artificial intelligence (AI) methods for marine-related corrosion prediction and detection: (1) predictive maintenance approaches and (2) computer vision and image processing approaches. Furthermore, a brief description of AI is described. The outcomes of this review will bring forward new knowledge about AI and the development of prediction models which can avoid unexpected failures during corrosion detection and maintenance. Moreover, it will expand the understanding of computer vision and image processing approaches for accurately detecting corrosion in images and videos.
Evidence of the Intrusion of the Oceanic Lightfish (Vinciguerria nimbaria) into Korean Waters Based on High-Throughput Sequencing of Mixed Fish Eggs
Authors: Kim Sung , Chin Byung-sun , Wang Soon-young
Published: January 2023

The oceanic lightfish Vinciguerria nimbaria is a mesopelagic species found in tropical and subtropical waters. In this study, we collected a total of 266 mixed fish egg samples from 78 stations in Korean waters of the Northwest Pacific Ocean from January to November 2021, and analysed these samples for V. nimbaria using cytochrome c oxidase I (COI) metabarcoding. We detected V. nimbaria eggs five times in May and once in August, with 20 V. nimbaria eggs to be estimated among the 266 mixed samples, which consisted of 68,844 eggs. To verify the accuracy of the metabarcoding results, two samples consisting of 1 and 6 eggs (diameter, 0.82 ± 0.07 mm; n = 5), respectively, that were identified as V. nimbaria were reanalysed using partial COI regions with the Sanger sequencing method. COI sequences obtained using both sequencing methods showed 100% identity in the overlapping regions. The mixed eggs formed one clade with V. nimbaria (average pairwise genetic distance, 0.002 ± 0.003; n = 7) in a phylogenetic ML tree based on the mitogenome (2 rRNAs and 13 protein-coding genes) of order Stomiiformes, including partial COIs from the mixed egg samples. The pairwise genetic distances in this clade were smaller than that of Stomiiformes (0.468 ± 0.081), except for V. nimbaria. These eggs represent direct evidence of the intrusion or distribution of adult V. nimbaria, an unrecorded species, in Korean waters.
The Analysis of Cavitation Flow and Pressure Pulsation of Bi-Directional Pump
Authors: Liu Haiyu , Tang Fangping , Shi Lijian , Dai Liang , Shen Jie , Liu Jian
Published: January 2023

A bi-directional pump is designed by using S-shaped hydrofoil, is the most convenient way to achieve bi-directional operation. In this paper, high-speed photography is used to visualize the flow field characteristics of the bidirectional pump under different cavitation numbers, and the flow field changes caused by cavitation are quantitatively analyzed in combination with the pressure pulsation sensor. The results show that the operation efficiency of the bidirectional pump in reverse operation is lower than that in forward operation. Tip clearance cavitation occurs on both suction and pressure surfaces of the impeller under reverse operation and large flow. In reverse operation, the influence of guide vane on the main frequency of pressure pulsation in the impeller is obvious. The quasi-periodic vertical cavitation flow phenomenon increases the amplitude of pressure pulsation in the impeller and becomes the main component of the internal flow in the bidirectional axial flow pump.
Nitrogen Enriched Solid-State Cultivation for the Overproduction of Azaphilone Red Pigments by Penicillium sclerotiorum SNB-CN111
Authors: Hebra Téo , Eparvier Véronique , Touboul David
Published: January 2023

Azaphilones are microbial specialized metabolites employed as yellow, orange, red or purple pigments. In particular, yellow azaphilones react spontaneously with functionalized nitrogen groups, leading to red azaphilones. In this study, a new two-step solid-state cultivation process to produce specific red azaphilones pigments was implemented, and their chemical diversity was explored based on liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and a molecular network. This two-step procedure first implies a cellophane membrane allowing accumulating yellow and orange azaphilones from a Penicillium sclerotiorum SNB-CN111 strain, and second involves the incorporation of the desired functionalized nitrogen by shifting the culture medium. The potential of this solid-state cultivation method was finally demonstrated by overproducing an azaphilone with a propargylamine side chain, representing 16% of the metabolic crude extract mass.
Diagnosis of Aspergillosis in Horses
Authors: Dobiáš Radim , Jahn Petr , Tóthová Katarina , Dobešová Olga , Višňovská Denisa , Patil Rutuja , Škríba Anton , Jaworská Pavla , Škorič Miša , Podojil Libor , Kantorová Michaela , Mrázek Jakub , Krejčí Eva , Stevens David A. , Havlíček Vladimír
Published: January 2023

Invasive pulmonary aspergillosis (IPA) may be a rare cause of granulomatous pneumonia in horses. The mortality of IPA is almost 100%; direct diagnostic tools in horses are needed. Bronchoalveolar lavage fluid (BALF) and serum samples were collected from 18 horses, including individuals suffering from IPA (n = 1), equine asthma (EA, n = 12), and 5 healthy controls. Serum samples were collected from another 6 healthy controls. Samples of BALF (n = 18) were analyzed for Aspergillus spp. DNA, fungal galactomannan (GM), ferricrocin (Fc), triacetylfusarinin C (TafC), and gliotoxin (Gtx). Analysis of 24 serum samples for (1,3)-β-D-glucan (BDG) and GM was performed. Median serum BDG levels were 131 pg/mL in controls and 1142 pg/mL in IPA. Similar trends were observed in BALF samples for GM (Area under the Curve (AUC) = 0.941) and DNA (AUC = 0.941). The fungal secondary metabolite Gtx was detected in IPA BALF and lung tissue samples (86 ng/mL and 2.17 ng/mg, AUC = 1).
Complement, but Not Platelets, Plays a Pivotal Role in the Outcome of Mucormycosis In Vivo
Authors: Harpf Verena , Rambach Günter , Parth Nadia , Neurauter Magdalena , Fleischer Verena , Lackner Michaela , Lass-Flörl Cornelia , Würzner Reinhard , Speth Cornelia
Published: January 2023

Background: Mucormycetes, a heterogeneous group of fungi, induce a life-threatening disease called mucormycosis. Immune deficiencies represent a major risk factor; hence, we wanted to illuminate the role of complement and platelets in the defense against mucormycetes. Methods: Rhizopus arrhizus (Ra), Rhizopus microsporus (Rm), Lichtheimia ramosa (Lr), Lichtheimia corymbifera (Lc), Rhizomucor pusillus (Rmp), and Mucor circinelloides (Mc) spores were opsonized with human and mouse serum, and C1q, C3c, and terminal complement complex (C5b-9) deposition was measured. Additionally, thrombocytopenic, C3-deficient, or C6-deficient mice were intravenously infected with selected isolates. Survival and immunological parameters were monitored, and fungal burden was determined and compared to that of immunocompetent and neutropenic mice. Results: In vitro experiments showed significant differences in complement deposition between mucormycetes. Mc isolates bound up to threefold more human C5b-9 than other mucormycetes. Lr, Lc, and Mc bound high levels of murine C3c, whereas human C3c deposition was reduced on Mc compared to Lr and Lc. Murine C3c deposition negatively correlated with virulence. Complement deficiencies and neutropenia, but not thrombocytopenia, were shown to be a risk factor for a lethal outcome. Conclusion: Complement deposition varies between mucormycetes. Additionally, we demonstrated that complement and neutrophilic granulocytes, but not platelets, play an important role in a murine model of disseminated mucormycosis.
A Study about Who Is Interested in Stock Splitting and Why: Considering Companies, Shareholders, or Managers
Authors: Chen Jiaquan , Ausloos Marcel
Published: January 2023

There are many misconceptions around stock prices and stock splits, and the behavior of shareholders, investors, and managers based on such information, due to a number of confounding factors. This paper tests a few hypotheses using a selected database, concerning the question “Is the stock split attractive for companies?”—in another words, “Why do companies split their stock?”, “Why do managers split their stock?” (sometimes for no benefit), and “Why do shareholders agree with such decisions?”. We contribute to the existing knowledge through a discussion of a random code selection of nine events in recent (selectively chosen) years, observing the role of information asymmetries, and the returns and traded volumes before and after the event. Therefore, calculating the beta for each sample, it is found that stock splits (i) affect the market and slightly enhance the trading volume in the short term, (ii) increase the shareholder base for their firm, and (iii) have a positive effect on the liquidity of the market. We concur that stock-splitting announcements can reduce the level of information asymmetries. Investors readjust their beliefs in the firm, although most of the firms are mispriced in the stock split year.
Mapping Priority Areas for Connectivity of Yellow-Winged Darter (Sympetrum flaveolum, Linnaeus 1758) under Climate Change
Authors: Rincón Víctor , Velázquez Javier , Gülçin Derya , López-Sánchez Aida , Jiménez Carlos , Özcan Ali Uğur , López-Almansa Juan Carlos , Santamaría Tomás , Sánchez-Mata Daniel , Çiçek Kerim
Published: January 2023

The yellow-winged darter (Sympetrum flaveolum Linnaeus, 1758, Odonata), which is associated with high mountain areas, can be considered a flagship species. Due to climate change, its natural range will be negatively affected. In this study, we propose global potential distributions for this species up to the year 2100, considering four time periods (2021–2040, 2041–2060, 2061–2080, and 2081–2100) and three shared socioeconomic pathways (optimistic—SSP245, middle of the road—SSP370, and worst—SSP585), by using an ecological niche model to produce two sets of distribution models (80% to 100% and 60% to 100%). It is foreseen that in the worst of the considered climate scenario (SSP585– 2100 year), the distribution of this species could be reduced by almost half, which could pose a risk for the species and provoke the shift from vulnerable to endangered. An analysis of connectivity has also been carried out for all the studied scenarios by applying the MSPA and PC indices, showing that the core habitat of this species will become more important, which is consistent with the decrease in the distribution range. Over time, the importance of the most valuable connectors will increase, implying a greater risk of some populations becoming isolated.
Exploring the Spatial Heterogeneity and Influence Factors of Daily Travel Carbon Emissions in Metropolitan Areas: From the Perspective of the 15-min City
Authors: Guo Liang , Cheng Wenjun , Liu Chang , Zhang Qinghao , Yang Shuo
Published: January 2023

Most of the residents’ daily travel is concentrated within their 15-min walking distance. In China, derived from the 15-min city concept, the 15-min walkable area is often referred to as the 15-min pedestrian-scale neighborhood, and it has become a basic planning unit. Understanding the factors that influence the built environment of the 15-min pedestrian-scale neighborhood on the residents’ daily travel carbon emissions is critical to reduce urban carbon emissions. There may be spatial heterogeneity in daily travel carbon emissions as a dependent variable due to the spatial heterogeneity of built environment factors. Therefore, this study used data from the Wuhan City Resident Travel Survey to describe the spatial pattern of daily travel carbon emissions among Wuhan residents. The study examined the spatial heterogeneity of daily travel carbon emissions and explored the spatial differentiation of the built environment’s impact on daily travel carbon emissions within the 15-min pedestrian-scale neighborhood of the residents using spatial autocorrelation analysis and multi-scale geo-weighted regression (MGWR). The results indicate that Wuhan residents’ daily travel carbon emissions show an increasing circle structure from the center outward. In general, built environment elements in the 15-min pedestrian-scale neighborhood are closely related to the daily travel carbon emissions, and the direction and degree of impact of the built environment varies spatially. This study provides empirical evidence for controlling transportation carbon emissions.
A Critical Review of the Psychomotor Agitation Treatment in Youth
Authors: Tripodi Beniamino , Matarese Irene , Carbone Manuel Glauco
Published: January 2023

(1) Background: To systematically review evidence on the safety and efficacy of psychopharmacological treatments available for psychomotor agitation (PA) in children and adolescents. (2) Methods: Studies assessing the safety and efficacy of psychopharmacological treatments for acute PA in children and adolescents that were published between January 1984 and June 2022 on PubMed were systematically reviewed. We included: (i) papers that presented a combination of the search terms specified in the “Search strategy” sub-paragraph; (ii) manuscripts in English; (iii) original papers; (iv) prospective or retrospective/observational studies and experimental or quasi-experimental reports. The exclusion criteria were: (i) review papers; (ii) non-original studies including editorials and book reviews; (iii) studies not specifically designed and focused on the selected topic. (3) Results: We selected 42 papers: 11 case series (11/42, 26.19%), 8 chart reviews (8/42, 19.05%), 8 case reports (8/42, 19.05%), 6 double-blind placebo-controlled randomized studies (6/42, 14.29%), 4 double-blind controlled randomized studies (4/42, 9.52%), 4 open-label trials (4/42, 9.52%) and 1 case control (1/42, 2.38%). (4) Conclusions: The drugs most frequently used to treat agitation in children and adolescents were ziprasidone, risperidone, aripiprazole, olanzapine and valproic acid. Further studies are needed to evaluate the efficacy/safety ratio, considering the limited number of observations in this field.
Clear Aligners and Smart Eye Tracking Technology as a New Communication Strategy between Ethical and Legal Issues
Authors: Putrino Alessandra , Marinelli Enrico , Raso Mario , Calace Valeria , Zaami Simona
Published: January 2023

Smart eye-tracking technology (SEET) that determines visual attention using smartphones can be used to determine the aesthetic perception of different types of clear aligners. Its value as a communication and comprehension tool, in addition to the ethical and legal concerns which it entails, can be assessed. One hundred subjects (50 F, 50 M; age range 15–70) were equally distributed in non-orthodontic (A) and orthodontic (B) groups. A smartphone-based SEET app assessed their knowledge of and opinions on aligners. Subjects evaluated images of smiles not wearing aligners, with/without attachments and with straight/scalloped gingival margins, as a guided calibration step which formed the image control group. Subsequently, the subjects rated the same smiles, this time wearing aligners (experimental images group). Questionnaire data and average values for each group of patients, and images relating to fixation times and overall star scores, were analyzed using these tests: chi-square, t-test, Mann–Whitney U, Spearman’s rho, and Wilcoxon (p < 0.05). One-way ANOVA and related post-hoc tests were also applied. Orthodontic patients were found to be better informed than non-orthodontic patients. Aesthetic perception could be swayed by several factors. Attachments scored lower in aesthetic evaluation. Lips distracted attention from attachments and improved evaluations. Attachment-free aligners were better rated overall. A more thorough understanding as to the opinions, expectations and aesthetic perception of aligners can improve communication with patients. Mobile SEET is remarkably promising, although it does require a careful medicolegal risk–benefit assessments for responsible and professional use.
Difficulties in Adaptation of the Mother and Newborn via Cesarean Section versus Natural Birth—A Narrative Review
Authors: Lupu Vasile Valeriu , Miron Ingrith Crenguta , Raileanu Anca Adam , Starcea Iuliana Magdalena , Lupu Ancuta , Tarca Elena , Mocanu Adriana , Buga Ana Maria Laura , Lupu Valeriu , Fotea Silvia
Published: January 2023

Birth is a physiological act that is part of the morpho-functional economy of the maternal body. Each stage in the act of birth has a predetermined pathway that is neurohormonally induced and morpho-functionally established through specific and characteristic adaptations. Like maternity, childbirth also has an important impact on the maternal body as a biological structure and psycho-emotional behavior. Cesarean section performed at the request of the mother with no medical underlying conditions besides the prolonged hospitalization risk can also cause breathing problems in children, delayed breastfeeding, and possible complications in a future pregnancy. Vaginal birth remains the path of choice for a physiological evolution pregnancy. Although erroneously considered safe and easy today, cesarean section delivery must remain an emergency procedure or a procedure recommended for pregnancies where birth is a risk to the mother and to the child, as cesarean section itself is a risk factor for negative outcomes for both mother and baby. This review summarizes the impact that both cesarean section and natural birth have on mother and newborn in their attempt to adapt to postpartum events and extrauterine life.
A Synopsis of Current Theories on Drug-Induced Nephrotoxicity
Authors: Dobrek Lukasz
Published: January 2023

The overriding goal of the treatment of patients is its effectiveness and safety. However, all medications currently being used also exert some adverse pharmaceutical reactions, which may be regarded as an unintended but inevitable cost of pharmacotherapy. The kidney, as the main organ that eliminates xenobiotics, is an organ especially predisposed and vulnerable to the toxic effects of drugs and their metabolites during their excretion from the body. Moreover, some drugs (e.g., aminoglycosides, cyclosporin A, cisplatin, amphotericin B, and others) have a “preferential” nephrotoxicity potential, and their use is associated with an increased risk of kidney damage. Drug nephrotoxicity is, therefore, both a significant problem and a complication of pharmacotherapy. It should be noted that, currently, there is no generally recognized definition of drug-induced nephrotoxicity and no clear criteria for its diagnosis. This review briefly describes the epidemiology and diagnosis of drug-induced nephrotoxicity and characterizes its pathomechanisms, including immunological and inflammatory disturbances, altered kidney blood flow, tubulointerstitial injury, increased lithogenesis–crystal nephropathy, rhabdomyolysis, and thrombotic microangiopathy. The study also lists the basic drugs with nephrotoxicity potential and provides a short overview of the preventive methods for reducing the risk of drug-related kidney damage developing.
Chronic Vulvar Pain and Health-Related Quality of Life in Women with Vulvodynia
Authors: Patla Gabriela , Mazur-Bialy Agnieszka I. , Humaj-Grysztar Magdalena , Bonior Joanna
Published: January 2023

The aim of this study was to investigate the severity of chronic vulvar pain in women with vulvodynia and its impact on their health-related quality of life (QL). The study group consisted of 76 women aged 19 to 58. The study was carried out using the diagnostic survey method, i.e., (1) the questionnaire technique, comprising (A) the author’s questionnaire (76 questions) and (B) the WHOQOL-BREF questionnaire, and (2) the VAS. When analyzing the severity of vulvar pain on the VAS, the highest proportion of women rated it at level 6 (23.68%). This was significantly determined by certain personal characteristics (age < 25 years old) and sociodemographic characteristics (marital status: unmarried women, divorcees, widows; high school education), each at p < 0.05. Vulvodynia causes a significant deterioration (64.47%) in QL, which is mainly caused by a reduction in the ability to perform activities of daily living (27.63%) and a decrease in sexual satisfaction (27.63%). The level of stress significantly exacerbates pain (p < 0.05). The severity correlates significantly (p < 0.05) and negatively (r < 0) with QL perception, which was rated worst in the physical domain. The use of treatment resulted in a significant improvement in the physical and psychological domains (p < 0.05), and the latter was particularly influenced by physiotherapy (p < 0.05).
Investigation of The Effects of Oxytocin Administration Timing on Postpartum Hemorrhage during Cesarean Section
Authors: Gök Soner , Kaleli Mehmet Babür
Published: January 2023

Background and Objectives: To determine and compare the effects of the timing of oxytocin administration (routinely used for intraoperative uterotonic purposes in cesarean section (CS) deliveries in our clinic) on the severity of postpartum hemorrhage following CS. Materials and Methods: All study participants (n = 216) had previous cesarean deliveries, were 38–40 weeks pregnant, and had CS planned under elective conditions. The cases were randomly divided into two groups: one group (n = 108) receiving oxytocin administration before the removal of the placenta (AOBRP) and another group (n = 108) receiving oxytocin administration after the removal of the placenta (AOARP). In all cases, the placenta was removed using the manual traction method. The standard dose of oxytocin is administered as an intravenous (IV) push of 3 international units (IU); simultaneously, 10 IU of oxytocin is added to 1000 cc isotonic fluid and given as an IV infusion at a rate of 250 cc/h. All methods and procedures applied to both groups were identical, except for the timing of administration of the standard oxytocin dose. Age, body mass index (BMI), parity, gestational week, preoperative hemoglobin (HB) and hematocrit (HTC), postoperative 6th and 24th hour HB-HTC, intraoperative hemorrhage, additional uterotonic need during cesarean section, postoperative hemorrhage (number of pads), need for blood transfusion during or after cesarean section, cesarean section time, and postpartum newborn baby weight were evaluated. Results: Age (year), BMI (kg/m2), parity, gestational week, surgical time, and newborn weight (g) did not differ between the groups (p > 0.05). The AOBRP group had significantly higher postoperative 6th hour HB and HTC and postoperative 24th hour HB and HTC values (p < 0.05). The intraoperative hemorrhage level was higher in the AOARP group (p = 0.000). Conclusions: The administration of oxytocin before placenta removal did not change the volume of bleeding in the postoperative period but significantly reduced the volume of bleeding in the intraoperative period. Therefore, in the postoperative period, the HB and HTC values of the AOBRP group were higher than those of the AOARP group.
Improvement of Advanced Sample Preparation Systems for the Determination of Trace Ni in Seawater by Electro-Membranes
Authors: Pinto Juan , Mendiguchía Carolina , López-López José , Martín-Barata Mabel , Silva Macarena , Moreno Carlos
Published: January 2023

Due to its important environmental role, the analysis of trace metals in natural waters is attracting increasing attention; consequently, faster and more accurate analytical methods are now needed to reach even lower limits of detection. In this work, we propose the use of electro-membrane extraction (EME) to improve analytical methods based on hollow fiber liquid phase micro-extraction (HFLPME). Specifically, an EME-based method for the determination of trace Ni in seawater has been developed, using an HFLPME system with di-2-ethylhexyl phosphoric acid (DEHPA) in kerosene as a chemical carrier, followed by instrumental determination by graphite furnace atomic absorption spectroscopy (GFAAS). Under optimum conditions, Ni was pre-concentrated 180 ± 17 times after 15 min, using sample pH = 5.5, the concentration of DEHPA 0.9 M in the liquid membranes, and 1.9 M HNO3 in the acceptor solution, as well as an electric potential of 25 V with the sample being stirred at 500 rpm. When compared with other HFLPME systems for pre-concentration of trace Ni in seawater in the absence of electric potential, the enrichment factor was improved 2.2 times, while the time of extraction was reduced an 89%. The limit of detection of the new method was 23.3 ng L−1, and both its applicability and accuracy were successfully evaluated by analyzing Ni concentration in a seawater-certified reference material (BCR-403), showing the reliability of EME for sample preparation in the determination of trace metals in marine water samples.
Eutectoid Transformation Kinetics of FeO under N2 and Air Atmospheres
Authors: Wang Hao , Cao Guangming , Li Silin , Zhao Wencong , Liu Zhenyu
Published: January 2023

The effect of different oxygen content on eutectoid transformation kinetics in FeO were studied in this paper. Thermogravimetric analysis was employed to investigate the eutectoid reaction in the oxide formed on pure Fe after being exposed to air at 900 °C for 10 min. The oxidized specimens were held isothermally in N2 and air from 100 s to 10,000 s in the temperature range of 350 to 550 °C, and the morphologies in FeO were observed by electron probe microanalysis. The results of the eutectoid transformation are statistically analyzed, and the dynamic model of the FeO eutectoid transformation is established based on the Johnson-Mehl-Avrami-Kolmogorov equation. Combined with the measured values and the calculation results, the time of eutectoid reaction in air is earlier than that in N2. Under the experimental conditions, the formation of Fe3O4 seams can occur at the interface of the FeO-substrate after the eutectoid reaction has begun, which means the eutectoid reaction is more determined by local ion concentration changes. At the Fe3O4-FeO interface, there is a high concentration enrichment of Fe ions, giving priority to the formation of Fe-rich FeO, which makes the eutectoid phase transition time earlier than in N2 conditions.
A Novel Miniature and Selective CMOS Gas Sensor for Gas Mixture Analysis—Part 3: Extending the Chemical Modeling
Authors: Goikhman Boris , Avraham Moshe , Bar-Lev Sharon , Stolyarova Sara , Blank Tanya , Nemirovsky Yael
Published: January 2023

This is the third part of the paper presenting a miniature, combustion-type gas sensor (dubbed GMOS) based on a novel thermal sensor (dubbed TMOS). The TMOS is a micromachined CMOS-SOI transistor, which acts as the sensing element and is integrated with a catalytic reaction plate, where ignition of the gas takes place. The first part was focused on the chemical and technological aspects of the sensor. In Part 2, the emphasis was on the physical aspects of the reaction micro-hot plate on which the catalytic layer is deposited. The present study focuses on applying several advanced simulation tools, which extend our understanding of the GMOS performance, as well as pellistor sensors in general. The three main challenges in simulating the performance are: (i) how to define the operating temperature based on the input parameters; (ii) how to measure the dynamics of the temperature increase during cyclic operation at a given duty cycle; (iii) how to model the correlation between the operating temperature and the sensing response. The simulated and analytical models and measured results are shown to be in good agreement.
Accelerated Adaptive Laboratory Evolution by Automated Repeated Batch Processes in Parallelized Bioreactors
Authors: Bromig Lukas , Weuster-Botz Dirk
Published: January 2023

Adaptive laboratory evolution (ALE) is a valuable complementary tool for modern strain development. Insights from ALE experiments enable the improvement of microbial cell factories regarding the growth rate and substrate utilization, among others. Most ALE experiments are conducted by serial passaging, a method that involves large amounts of repetitive manual labor and comes with inherent experimental design flaws. The acquisition of meaningful and reliable process data is a burdensome task and is often undervalued and neglected, but also unfeasible in shake flask experiments due to technical limitations. Some of these limitations are alleviated by emerging automated ALE methods on the μL and mL scale. A novel approach to conducting ALE experiments is described that is faster and more efficient than previously used methods. The conventional shake flask approach was translated to a parallelized, L scale stirred-tank bioreactor system that runs controlled, automated, repeated batch processes. The method was validated with a growth optimization experiment of E. coli K-12 MG1655 grown with glycerol minimal media as a benchmark. Off-gas analysis enables the continuous estimation of the biomass concentration and growth rate using a black-box model based on first principles (soft sensor). The proposed method led to the same stable growth rates of E. coli with the non-native carbon source glycerol 9.4 times faster than the traditional manual approach with serial passaging in uncontrolled shake flasks and 3.6 times faster than an automated approach on the mL scale. Furthermore, it is shown that the cumulative number of cell divisions (CCD) alone is not a suitable timescale for measuring and comparing evolutionary progress.
Diversity and Comparison of Intestinal Desulfovibrio in Patients with Liver Cirrhosis and Healthy People
Authors: Lu Gexue , Zhang Yu , Ren Yilin , Shi Jin-Song , Xu Zheng-Hong , Geng Yan
Published: January 2023

Desulfovibrio belongs to Sulfate-reducing bacteria (SRB), which are widely present in anaerobic environments, including the human gut. Desulfovibrio has been associated with many human diseases, including chronic liver disease. However, the characteristics and difference of Desulfovibrio from fecal samples of healthy volunteers (HV) and patients with liver cirrhosis (LC) have not been fully elucidated. Here, we isolated Desulfovibrio from the feces of 6 HV and 9 LC, and 88 Desulfovibrio strains were obtained. In the feces of HV, 55% of isolated strains were D. desulfuricans, followed by D. intestinalis (15%), D. simplex (11%), D. piger (9%), D. legallii (4%), Cupidesulfovibrio oxamicus (4%) and D. fairfieldensis (2%). However, only D. desulfuricans (60%) and C. oxamicus (40%) were isolated from fecal samples of patients with LC. Our results suggest that there was a significant difference in the desulfurization ability and the H2S production ability of different Desulfovibrio. Desulfovibrio. Furthermore, we found that Desulfovibrio isolated from the patients with LC generally had a higher hydrogen sulfide production capacity, gastrointestinal tolerance, and levels of antibiotic resistance than the same species isolated from HV. Our findings suggested that Desulfovibrio may be associated with the occurrence and development of liver cirrhosis.