bluuebunny/crossref_metadata_2025 huggingface dataset:
Combining Abilities and Heterotic Patterns among Early Maturing Maize Inbred Lines under Optimal and Striga-Infested Environments
Authors: Adu Gloria Boakyewaa , Badu-Apraku Baffour , Akromah Richard , Awuku Frederick Justice
Published: December 2022

Information on the general combining ability of inbred lines and the specific combining ability of hybrid combinations is crucial for successful hybrid development. The objectives of this study were to (i) determine the combining ability of thirty selected early maturing maize inbred lines under Striga-infested and optimal environments, (ii) classify the inbred lines into heterotic groups using the general combining ability effects of multiple traits (HGCAMT) and the single nucleotide polymorphism genetic distance (SNP- GD) methods, and (iii) assess the effectiveness of the heterotic grouping methods. One hundred and fifty single-cross hybrids were generated from the thirty inbred lines using the North Carolina Design II mating method. The hybrids and six local check varieties were tested across optimal and Striga-infested environments in Ghana and Nigeria in 2016 and 2017. The inheritance of grain yield was controlled by the non-additive gene action under both environments and the additive gene action across the two research environments. The non-additive gene action modulated the inheritance of measured traits under Striga-infested environments, except for the Striga damage syndrome rating at 8 weeks after planting. Maternal effects were observed for most traits in each environment and across environments. The inbred lines TZEI 127 and TZEI 40 exhibited significant and positive GCA male and female effects for grain yield under each environment and across the two research environments, indicating the presence of favorable alleles for yield improvements. The SNP-GD heterotic grouping method was identified as the most adequate in grouping the thirty inbred lines.
Complete Plastome of Physalis angulata var. villosa, Gene Organization, Comparative Genomics and Phylogenetic Relationships among Solanaceae
Authors: Zhan Xiaori , Zhang Zhenhao , Zhang Yong , Gao Yadi , Jin Yanyun , Shen Chenjia , Wang Huizhong , Feng Shangguo
Published: December 2022

Physalis angulata var. villosa, rich in withanolides, has been used as a traditional Chinese medicine for many years. To date, few extensive molecular studies of this plant have been conducted. In the present study, the plastome of P. angulata var. villosa was sequenced, characterized and compared with that of other Physalis species, and a phylogenetic analysis was conducted in the family Solanaceae. The plastome of P. angulata var. villosa was 156,898 bp in length with a GC content of 37.52%, and exhibited a quadripartite structure typical of land plants, consisting of a large single-copy (LSC, 87,108 bp) region, a small single-copy (SSC, 18,462 bp) region and a pair of inverted repeats (IR: IRA and IRB, 25,664 bp each). The plastome contained 131 genes, of which 114 were unique and 17 were duplicated in IR regions. The genome consisted of 85 protein-coding genes, eight rRNA genes and 38 tRNA genes. A total of 38 long, repeat sequences of three types were identified in the plastome, of which forward repeats had the highest frequency. Simple sequence repeats (SSRs) analysis revealed a total of 57 SSRs, of which the T mononucleotide constituted the majority, with most of SSRs being located in the intergenic spacer regions. Comparative genomic analysis among nine Physalis species revealed that the single-copy regions were less conserved than the pair of inverted repeats, with most of the variation being found in the intergenic spacer regions rather than in the coding regions. Phylogenetic analysis indicated a close relationship between Physalis and Withania. In addition, Iochroma, Dunalia, Saracha and Eriolarynx were paraphyletic, and clustered together in the phylogenetic tree. Our study published the first sequence and assembly of the plastome of P. angulata var. villosa, reported its basic resources for evolutionary studies and provided an important tool for evaluating the phylogenetic relationship within the family Solanaceae.
Isabelle de Charrière, Jane Austen, and Post-Enlightenment Fiction: Writing the Shared Humanity of Men and Women
Authors: Cossy Valérie
Published: December 2022

This article analyses Isabelle de Charrière and Jane Austen together in relation to the changing perception of humanity affecting European thinking at the end of the eighteenth century. Not only is Charrière’s rather lesser-known work likely to benefit from the comparison, but Austen’s novels also gain in philosophical depth when read alongside hers. Each one an outsider in her own world, Charrière and Austen wrote against the grain of inherited gender prejudices but also against the growing conservatism of binary orthodoxy around 1800. Adopting as writers a perspective freed from ‘feminine’ expectations, as opposed to the lady novelist embodied by the famous Isabelle de Montolieu, they endeavoured to invent characters and stories distinct from the pervasive definitions of the feminine and the masculine, giving readers the possibility of considering the shared humanity equally shaping men and women.
A Comparison between Open and Minimally Invasive Techniques for the Resection of Colorectal Liver Metastasis
Authors: Bartolini Ilenia , Fortuna Laura , Risaliti Matteo , Tirloni Luca , Buccianti Simone , Luceri Cristina , Ringressi Maria Novella , Batignani Giacomo , Taddei Antonio
Published: December 2022

The liver is the most common site of colorectal cancer metastasis. Liver surgery is a cornerstone in treatment, with progressive expansion of minimally invasive surgery (MIS). This study aims to compare short- and long-term outcomes of open surgery and MIS for the treatment of colorectal adenocarcinoma liver metastasis during the first three years of increasing caseload and implementation of MIS use in liver surgery. All patients treated between November 2018 and August 2021 at Careggi Teaching Hospital in Florence, Italy, were prospectively entered into a database and retrospectively reviewed. Fifty-one patients were resected (41 open, 10 MIS). Considering that patients with a significantly higher number of lesions underwent open surgery and operative results were similar, postoperative morbidity rate and length of hospital stay were significantly higher in the open group. No differences were found in the pathological specimen. The postoperative mortality rate was 2%. Mean overall survival and disease-free survival were 46 months (95% CI 42–50) and 22 months (95% CI 15.6–29), respectively. The use of minimally invasive techniques in liver surgery is safe and feasible if surgeons have adequate expertise. MIS and parenchymal sparing resections should be preferred whenever technically feasible.
Influencing Appropriate Statin Use in a Charity Care Primary Clinic
Authors: Manohar Hasitha Diana , Karkour Carole , Desai Rajesh N.
Published: December 2022

According to the American College of Cardiology/American Heart Association (ACC/AHA) new cholesterol management guidelines in 2019, statin regimen was prescribed to only about 46.4% and 30% of diabetes (DM) patients and patients with atherosclerotic cardiovascular disease (ASCVD), respectively. Atherosclerotic cardiovascular disease accounts for most deaths and disabilities in North America. This study argues that a systematic approach to identifying targeted interventions to adhere to the statin regimen for ASCVD is sparse in previous studies. This study seeks to address the research gap. Besides, the study argues that the statin regimen could improve cholesterol management with the enablers of pharmacy, providers, electronic medical records (E.M.R.), and patients. It paves the way for future research on cardiovascular and statin regimens from different perspectives. Current study has adopted the Qualitative observation method. Accordingly, the study approached the charity care primary clinic serving a large population in the northeastern part of the United States, which constitutes the project’s setting. The facility has 51 internal medicine residents. The facility has E.H.R., which is used by the clinical staff. Besides, providers use electronic medication prescribing (E-Scribe). Four PDSA cycles were run in six months. Here, the interventions were intensified during each subsequent cycle. The interventions were then incorporated into routine clinical practice. Based on the observation, the study found a 25% relative improvement by six months based on the baseline data of the appropriate intensity statin prescription for patients with ASCVD or DM by medical resident trainees in our single-center primary care clinic. A total of 77% of cardiovascular disease patients were found to be on an appropriate statin dose at baseline. On the other hand, the proportion of patients with DM who were on proper dose statin was 80.4%. According to the study’s findings, PDSA could result in a faster uptake and support of the ACC/AHA guidelines. Evidence indicates that overmedication of persons at low risk and time constraints are some of the most significant impediments to the greater use of prescription medications. Proactive panel management can help improve statins’ use by ensuring they are used appropriately.
What Primary Care Practitioners Need to Know about the New NICE Guideline for Myalgic Encephalomyelitis/Chronic Fatigue Syndrome in Adults
Authors: Kingdon Caroline , Lowe Adam , Shepherd Charles , Nacul Luis
Published: December 2022

The new NICE guideline for myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), published in October 2021, makes significant changes in treatment recommendations. It acknowledges the complexity of this chronic medical condition, which always impacts quality of life and can be profoundly disabling, recognising the prejudice and stigma that people with ME/CFS often experience in the absence of any specific diagnostic test. The guideline outlines steps for accurate diagnosis, recognising post-exertional malaise as a core symptom; importantly, ME/CFS can now be diagnosed after just 3 months in a bid to improve long-term health outcomes. It recommends the need for individual, tailored management by a multi-disciplinary team, ensuring that the wellbeing of the individual is paramount. The guideline makes clear that any programme based on fixed incremental increases in physical activity or exercise, for example, graded exercise therapy (GET), should not be offered as a treatment for ME/CFS and emphasises that cognitive behavioural therapy (CBT) should only be offered as a supportive intervention. Because of the rigorous methodology required by NICE Committee review and the inclusion of the testimony of people with lived experience as committee members, this guideline will influence the future diagnosis and management of ME/CFS in the UK and beyond.
Ergonomic Risk Factors for Musculoskeletal Disorders among Ethnic Lychee–Longan Harvesting Workers in Northern Thailand
Authors: Ong-Artborirak Parichat , Kantow Supakan , Seangpraw Katekaew , Tonchoy Prakasit , Auttama Nisarat , Choowanthanapakorn Monchanok , Boonyathee Sorawit
Published: December 2022

Musculoskeletal disorders (MSDs) are one of the leading causes of occupational injuries and disabilities. The purpose of this study was to assess the prevalence of MSDs and occupational factors affecting MSDs among ethnic lychee–longan harvesting workers in northern Thailand. A cross-sectional study was conducted in the areas of three upper northern provinces of Thailand. The study areas are located in the highlands and rural plains, where many ethnic minority groups live, including Indigenous, Mien, Karen, and Lua. The majority of them work in a farm of perennial fruit trees, mainly lychee and longan. During the harvest season, 404 participants were recruited for the study using the convenience sampling technique. Data were collected using questionnaires that included general information, an ergonomic risk assessment, and a standardized Nordic questionnaire for assessing MSDs in 10 body parts. The average age of lychee–longan harvesting workers was 48.8 years. Almost all (99.5%) reported MSDs in one or more body regions in the previous seven days of work. The prevalence of MSDs was highest in the hands (82.9%), followed by the shoulders (82.2%) and the neck (79.7%). The total ergonomic risk scores, which included awkward posture, heavy carrying and lifting, repetitive activity, land slope, and equipment, were found to be significantly associated with MSDs in part of the neck (AOR = 1.17, 95%CI = 1.11–1.23), shoulder (AOR = 1.15, 95%CI = 1.10–1.21), elbow (AOR = 1.18, 95%CI = 1.12–1.24), hand (AOR = 1.12, 95%CI = 1.07–1.18), finger (AOR = 1.33, 95%CI = 1.24–1.44), upper back (AOR = 1.14, 95%CI = 1.09–1.20), lower back (AOR = 1.16, 95%CI = 1.11–1.22), hip (AOR = 1.11, 95%CI = 1.06–1.15), knee (AOR = 1.18, 95%CI = 1.12–1.24), and feet (AOR = 1.21, 95%CI = 1.15–1.28) when adjusting for ethnicity, sex, age, BMI, and work experience. Many parts of ethnic workers’ bodies have been affected by occupational injuries, with a high risk of upper extremity injury. As a result, using an ergonomic approach to improving the working environment and appropriate posture movement is very beneficial in preventing MSDs among ethnic harvesting workers.
Burnout and Depressive Symptoms in Healthcare Professionals: A Cross-Sectional Study in Saudi Arabia
Authors: Alwhaibi Monira , Alhawassi Tariq M. , Balkhi Bander , Al Aloola Noha , Almomen Aliyah A. , Alhossan Abdulaziz , Alyousif Sarah , Almadi Bana , Bin Essa Maryam , Kamal Khalid M.
Published: December 2022

Objectives. The study objectives were to examine the prevalence of burnout among healthcare professionals, analyze the association of depression and burnout among healthcare professionals, and explore the factors related to burnout. Methods. A prospective cross-sectional study using a validated questionnaire was conducted among healthcare professionals in a tertiary teaching hospital in Saudi Arabia’s central region. The Maslach Burnout Inventory (MBI) questionnaire was used to measure burnout through emotional exhaustion, depersonalization, and personal accomplishment. Descriptive and inferential statistics were carried out using SAS version 9.4. Results. The study sample was composed of 139 healthcare professionals. Around 48% of the study sample were nurses, 26% were physicians, 19% were pharmacists, and 6% were other healthcare professionals. About 61% screened positive for depression. Overall, one third of the participants had a high risk of burnout. Around 61.8% of the participants were in the high-risk group of the EE, 58.3% of the DP, and 41.0% of the PA subscales. Scores for the overall MBI were significantly different between various age groups, gender, those with social and financial responsibility, income, job titles, or years of experience. A higher risk of burnout in all subscales was observed among those with depression. Conclusions. A high risk of burnout was observed among healthcare professionals. The level of burnout was connected to workplace factors and the presence of depression. The burnout suffering among these healthcare professionals underlines the need to study further how to reduce the factors that contribute to burnout and the impact of interventions to reduce healthcare professionals’ burnout levels. The burnout scientific literature would benefit from further high-quality research with larger samples using longitudinal study designs to identify the causal risk factors.
Cold-Water Immersion and Sports Massage Can Improve Pain Sensation but Not Functionality in Athletes with Delayed Onset Muscle Soreness
Authors: Angelopoulos Pavlos , Diakoronas Anastasios , Panagiotopoulos Dimitrios , Tsekoura Maria , Xaplanteri Panagiota , Koumoundourou Dimitra , Saki Farzaneh , Billis Evdokia , Tsepis Elias , Fousekis Konstantinos
Published: December 2022

This study aimed to investigate the effects of cold-water immersion (CWI) and sports massage on delayed-onset muscle soreness (DOMS) in amateur athletes. Sixty male amateur athletes were randomised into four equal groups (n = 15) receiving either CWI, sports massage, their combination, or served as controls after applying plyometric training to their lower extremities. The main outcomes measures were pain, exertion, rectus femoris perimeter, knee flexion range of motion, knee extensors isometric strength and serum creatine phosphokinase (CPK) levels examined before the plyometric training, immediately after the treatment, and 24, 48 and 72 h post exercise. We observed no significant differences between study groups in the most tested variables. CWI improved pain compared to the combined application of CWI and sports massage, and the control group both on the second and third day post exercise. Sports massage combined with CWI also led to a significant reduction in pain sensation compared to the control group. In conclusion the treatment interventions used were effective in reducing pain but were unable to affect other important adaptations of DOMS. Based on the above, sports scientists should reconsider the wide use of these interventions as a recovery strategy for athletes with DOMS.
Using xPIRT to Record Pharmacy Interventions: An Observational, Cross-Sectional and Retrospective Study
Authors: Baptista Rafael , Williams Mary , Price Jayne
Published: December 2022

Medication errors and omissions can potentially cause harm, prolong a hospital stay, lead to co-morbidities and even death. Pharmacy interventions (PI) ensure that these errors are identified and addressed, leading to improved patient safety and prescriber practice. Particularly in community hospitals, many only having general practitioners and not specialist doctors in their medical teams, PIs assume a strategic role. The PIs recorded throughout 8 months (between November 2021 and June 2022) in the community hospital wards in Powys, Wales, UK, using xPIRT (Pharmacy Intervention Recording Tool), a new pharmacy intervention record toolkit, were subjected to a retrospective analysis. The data were organised by location, drug, severity, acceptance, cost avoidance and intervention type. Significant prescribing errors were identified, which can potentially be different from those recorded in acute settings. Our results also informed on the need for integrated electronic prescribing systems paired with a PI recording tool to address effectively prescribing inaccuracies. Overall, this study was able to identify pharmacy teams as key to improve patient safety and care while contributing to significant cost-savings, through the recording of PI using xPIRT.
Development, Application, and Performance of Artificial Intelligence in Cephalometric Landmark Identification and Diagnosis: A Systematic Review
Authors: Junaid Nuha , Khan Niha , Ahmed Naseer , Abbasi Maria Shakoor , Das Gotam , Maqsood Afsheen , Ahmed Abdul Razzaq , Marya Anand , Alam Mohammad Khursheed , Heboyan Artak
Published: December 2022

This study aimed to analyze the existing literature on how artificial intelligence is being used to support the identification of cephalometric landmarks. The systematic analysis of literature was carried out by performing an extensive search in PubMed/MEDLINE, Google Scholar, Cochrane, Scopus, and Science Direct databases. Articles published in the last ten years were selected after applying the inclusion and exclusion criteria. A total of 17 full-text articles were systematically appraised. The Cochrane Handbook for Systematic Reviews of Interventions (CHSRI) and Newcastle-Ottawa quality assessment scale (NOS) were adopted for quality analysis of the included studies. The artificial intelligence systems were mainly based on deep learning-based convolutional neural networks (CNNs) in the included studies. The majority of the studies proposed that AI-based automatic cephalometric analyses provide clinically acceptable diagnostic performance. They have worked remarkably well, with accuracy and precision similar to the trained orthodontist. Moreover, they can simplify cephalometric analysis and provide a quick outcome in practice. Therefore, they are of great benefit to orthodontists, as with these systems they can perform tasks more efficiently.
Association of Vitamin C Treatment with Clinical Outcomes for COVID-19 Patients: A Systematic Review and Meta-Analysis
Authors: Huang Wen Yan , Hong Jiyoun , Ahn Sung-Il , Han Bok Kyung , Kim Young Jun
Published: December 2022

Background: Vitamin C is an essential nutrient that serves as an antioxidant and is known to reduce the inflammatory response associated with pneumonia and acute respiratory distress syndrome in patients with the coronavirus disease (COVID-19), but its clinical effects remain controversial. Methods: This study aimed to investigate the therapeutic effect of vitamin C administration on the clinical outcomes of COVID-19 patients through a systematic review and meta-analysis. Results: Nineteen studies were selected, of which 949 participants administered vitamin C were in the intervention group, and 1816 participants were in the control group. All-cause mortality, hospitalization duration, length of intensive care unit stay, and ventilation incidence in COVID-19 patients were analyzed. The intervention group tends to have a lower risk ratio (RR = 0.81, 95% CI: 0.62 to 1.07; I2 = 58%; Q = 40.95; p < 0.01) in all-cause mortality than the control group. However, there were no significant differences in ventilation incidence, hospitalization duration, and length of ICU stay between the two groups. In the subgroup analysis for all-cause mortality, the risk ratio for RCT as study design, combination therapy, of vitamin C was lower than that of the combination therapy with other agents. A moderate dosage showed a lower RR than a higher dose. Conclusion: The results suggest that vitamin C may lower mortality in COVID-19 patients, but further large-scale studies are required to assess the role of vitamin C in the treatment of COVID-19.
Identification of Phenological Growth Stages of Four Morus Species Based on the Extended BBCH-Scale and Its Application in Fruit Development with Morphological Profiles and Color Characteristics
Authors: Mo Rongli , Zhang Na , Hu Die , Jin Qiang , Li Jinxin , Dong Zhaoxia , Zhu Zhixian , Li Yong , Zhang Cheng , Yu Cui
Published: December 2022

Mulberry (Morus L.; Moraceae; Rosales) is an economically important tree with a long history in China and valued for its rich nutrient, flavor content, medicinal value and useful ecological functions. Morus species are widely distributed in Asia, Europe, North and South America and Africa and exhibit obvious differences in morphological characteristics and phenological stages due to being distributed in diverse climate zones. The morphological description of the phenological stages of black mulberry (Morus nigra L.) has been established in Mediterranean climates and this study extended the BBCH scale for application in different Morus species, especially for mulberry trees grown in subtropical monsoon climates. In this study, we used the BBCH (Biologische Bundesanstalt, Bundessortenamt and CHemische Industrie) scale to describe in detail the phenological growth stages for Morus species in Wuhan, China (a subtropical monsoon climate). Based on this general scale, eight principal stages, i.e., bud, leaf and shoot development stage, inflorescence emergence stage, flowering stage, fruit development stage, fruit maturation stage, senescence and beginning of dormancy stage, were described. We provide photographic images of some primary and secondary developmental stages to better define and standardize morphological characteristics and phenological descriptions of these mulberries (Morus spp.). In addition, because the color of mulberry fruit varies from white, purple, black, or pink to red at maturity, with mostly monosexual flowers, we have also presented detailed descriptions of morphological traits of flower sexuality and fruit color among these four species. In addition, two late phenological germplasms (late bud sprouting associated with late blooming and late fruit ripening) were screened out according to the BBCH scale. Overall, this study will contribute to advance the field of mulberry breeding and implementation of agronomic practices and facilitate convenient communication between mulberry cultivators and researchers in different areas.
Personality, Risk Perceptions, and Health Behaviors: A Two-Wave Study on Reciprocal Relations in Adults
Authors: Thøgersen-Ntoumani Cecilie , Stenling Andreas , Izett Esther , Quested Eleanor
Published: December 2022

The aim of the study was to examine reciprocal associations between risk perceptions for cardiovascular disease and health behaviors (i.e., physical activity, fruit/vegetable consumption), while accounting for key personality characteristics in middle-aged adults. Participants (N = 297; M (SD) age = 51 (6.95); 72.4% female) completed online questionnaires assessing risk perceptions, physical activity, fruit/vegetable consumption, and personality (conscientiousness and neuroticism) on two occasions, one week apart. Physical activity did not have a statistically significant effect on risk perception over time (b = −0.00, p = 0.227). However, fruit and vegetable consumption (b = −0.19, p = 0.006) and neuroticism (b = 0.22, p = 0.001) predicted risk perception. Risk perception did not have a significant effect on physical activity (b = −343.86, p = 0.147) or fruit/vegetable consumption (b = −0.08, p = 0.144) over time; however, neuroticism had significant and negative effects on physical activity (b = −520.84, p = 0.029) and fruit/vegetable consumption (b = −0.20, p = 0.001). High levels of neuroticism in middle age may hinder engagement in physical activity and consumption of fruit/vegetable behaviors and should therefore be targeted accordingly to increase protective health behaviors and reduce incidence of cardiovascular disease.
Risk Factors for Mortality in Emergently Admitted Patients with Acute Gastric Ulcer: An Analysis of 15,538 Patients in National Inpatient Sample, 2005–2014
Authors: Idris Maksat , Smiley Abbas , Patel Saral , Latifi Rifat
Published: December 2022

Background: Patients admitted emergently with a primary diagnosis of acute gastric ulcer have significant complications including morbidity and mortality. The objective of this study was to assess the risk factors of mortality including the role of surgery in gastric ulcers. Methods: Adult (18–64-year-old) and elderly (≥65-year-old) patients admitted emergently with hemorrhagic and/or perforated gastric ulcers, were analyzed using the National Inpatient Sample database, 2005–2014. Demographics, various clinical data, and associated comorbidities were collected. A stratified analysis was combined with a multivariable logistic regression model to assess predictors of mortality. Results: Our study analyzed a total of 15,538 patients, split independently into two age groups: 6338 adult patients and 9200 elderly patients. The mean age (SD) was 50.42 (10.65) in adult males vs. 51.10 (10.35) in adult females (p < 0.05). The mean age (SD) was 76.72 (7.50) in elderly males vs. 79.03 (7.80) in elderly females (p < 0.001). The percentage of total deceased adults was 1.9% and the percentage of total deceased elderly was 3.7%, a difference by a factor of 1.94. Out of 3283 adult patients who underwent surgery, 32.1% had perforated non-hemorrhagic ulcers vs. 1.8% in the non-surgical counterparts (p < 0.001). In the 4181 elderly surgical patients, 18.1% had perforated non-hemorrhagic ulcers vs. 1.2% in the non-surgical counterparts (p < 0.001). In adult patients managed surgically, 2.6% were deceased, while in elderly patients managed surgically, 5.5% were deceased. The mortality of non-surgical counterparts in both age groups were lower (p < 0.001). The multivariable logistic regression model for adult patients electing surgery found delayed surgery, frailty, and the presence of perforations to be the main risk factors for mortality. In the regression model for elderly surgical patients, delayed surgery, frailty, presence of perforations, the male sex, and age were the main risk factors for mortality. In contrast, the regression model for adult patients with no surgery found hospital length of stay to be the main risk factor for mortality, whereas invasive diagnostic procedures were protective. In elderly non-surgical patients, hospital length of stay, presence of perforations, age, and frailty were the main risk factors for mortality, while invasive diagnostic procedures were protective. The following comorbidities were associated with gastric ulcers: alcohol abuse, deficiency anemias, chronic blood loss, chronic heart failure, chronic pulmonary disease, hypertension, fluid/electrolyte disorders, uncomplicated diabetes, and renal failure. Conclusions: The odds of mortality in emergently admitted geriatric patients with acute gastric ulcer was two times that in adult patients. Surgery was a protective factor for patients admitted emergently with gastric perforated non-hemorrhagic ulcers.
A Comparison Study of Landslide Susceptibility Spatial Modeling Using Machine Learning
Authors: Nurwatik Nurwatik , Ummah Muhammad Hidayatul , Cahyono Agung Budi , Darminto Mohammad Rohmaneo , Hong Jung-Hong
Published: December 2022

One hundred seventeen landslides occurred in Malang Regency throughout 2021, triggering the need for practical hazard assessments to strengthen the disaster mitigation process. In terms of providing a solution for investigating the location of landslides more precisely, this research aims to compare machine learning algorithms to produce an accurate landslide susceptibility model. This research applies three machine learning algorithms composed of RF (random forest), NB (naïve Bayes), and KNN (k-nearest neighbor) and 12 conditioning factors. The conditioning factors consist of slope, elevation, aspect, NDVI, geological type, soil type, distance from the fault, distance from the river, river density, TWI, land cover, and annual rainfall. This research performs seven models over three ratios between the training and testing dataset encompassing 50:50, 60:40, and 70:30 for KNN and NB algorithms and 70:30 for the RF algorithm. This research measures the performance of each model using eight parameters (ROC, AUC, ACC, SN, SP, BA, GM, CK, and MCC). The results indicate that RF 70:30 generates the best performance, witnessed by the evaluation parameters ACC (0.884), SN (0.765), GM (0.863), BA (0.857), CK (0.749), MCC (0.876), and AUC (0.943). Overall, seven models have reasonably good accuracy, ranging between 0.806 and 0.884. Furthermore, based on the best model, the study area is dominated by high susceptibility with an area coverage of 51%, which occurs in the areas with high slopes. This research is expected to improve the quality of landslide susceptibility maps in the study area as a foundation for mitigation planning. Furthermore, it can provide recommendations for further research in splitting ratio scenarios between training and testing data.
ATPase Inhibitory Factor 1—A Novel Marker of Cellular Fitness and Exercise Capacity?
Authors: Domin Remigiusz , Pytka Michał , Niziński Jan , Żołyński Mikołaj , Zybek-Kocik Ariadna , Wrotkowska Elżbieta , Zieliński Jacek , Guzik Przemysław , Ruchała Marek
Published: December 2022

ATPase inhibitory factor 1 is a myokine inhibiting the hydrolytic activity of mitochondrial adenosine triphosphate synthase and ecto-F1-ATPase on the surface of many cells. IF1 affects ATP metabolism in mitochondria and the extracellular space and upregulates glucose uptake in myocytes; these processes are essential in physical activity. It is unknown whether the IF1 serum concentration is associated with exercise capacity. This study explored the association between resting IF1 serum concentration and exercise capacity indices in healthy people. IF1 serum concentration was measured in samples collected at rest in 97 healthy amateur cyclists. Exercise capacity was assessed on a bike ergometer at the successive stages of the progressive cardiopulmonary exercise test (CPET). IF1 serum concentration was negatively and significantly correlated with oxygen consumption, oxygen pulse, and load at various CPET stages. A better exercise capacity was associated with lower circulating IF1. IF1 may reflect better cellular/mitochondrial energetic fitness, but there is uncertainty regarding how IF1 is released into the intravascular space. We speculate that lower IF1 concentration may reflect a better cellular/mitochondrial integrity, as this protein is bound more strongly with ATPases in mitochondria and cellular surfaces in people with higher exercise capacity.
Ultrathin Films of Silver by Magnetron Sputtering
Authors: Filip Ana Violeta , Sava Bogdan Alexandru , Medianu Rares Victor , Boroica Lucica , Dinca Marius Catalin , Pascu Rovena , Tigau Nicolae , Andrei Andreea , Moldovan Antoniu , Dumitru Marius , Oane Mihai , Eftimie Mihai
Published: December 2022

The interest in ultrathin silver (Ag) films has increased due to their high surface plasmon resonance for coatings of only a few nm. Low roughness ultrathin films of 1 to 9 nm have been deposited on different substrates, such as polyethylene terephthalate (PET) and optical glass, using radio frequency (RF) magnetron sputtering. Films show good surface plasmon resonance up to 7 nm thickness, as revealed by the ultraviolet-visible (UV-Vis) spectra. The roughness of the films, investigated by Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM), is small, and one can conclude that depositions are smooth and homogeneous. The bandgap values decrease with film thickness. The refractive index of the films, calculated from ellipsometry measurements, leads to values of under 1 visible domain, with minima in the wavelength range of 400–600 nm. The results are useful for obtaining lower roughness ultrathin Ag films with good surface plasmon resonance for photonic applications.
Transcriptomic Analysis Insight into the Immune Modulation during the Interaction of Ophiocordyceps sinensis and Hepialus xiaojinensis
Authors: Tong Xinxin , Peng Ting , Liu Sukun , Zhang Daixi , Guo Jinlin
Published: December 2022

Ophiocordyceps sinensis (Berk.) is an entomopathogenic fungus that can infect the larva of the ghost moth, Hepialus xiaojinensis, causing mummification after more than one year. This prolonged infection provides a valuable model for studying the immunological interplay between an insect host and a pathogenic fungus. A comparative transcriptome analysis of pre-infection (L) and one-year post-infection (IL) larvae was performed to investigate the immune response in the host. Here, a total of 59,668 unigenes were obtained using Illumina Sequencing in IL and L. Among the 345 identified immune-related genes, 83 out of 86 immune-related differentially expressed genes (DEGs) had a much higher expression in IL than in L. Furthermore, the immune-related DEGs were classified as pathogen recognition receptors (PRRs), signal modulators or transductors, and immune effector molecules. Serpins and protease inhibitors were found to be upregulated in the late phase of infection, suppressing the host’s immune response. Based on the above analysis, the expression levels of most immune-related genes would return to the baseline with the immune response being repressed in the late phase of infection, leading to the fungal immunological tolerance after prolonged infection. Meanwhile, the transcriptomes of IL and the mummified larva (ML) were compared to explore O. sinensis invasion. A total of 1408 novel genes were identified, with 162 of them annotated with putative functions. The gene families likely implicated in O. sinensis pathogenicity have been identified, primarily including serine carboxypeptidase, peroxidase, metalloprotease peptidase, aminopeptidases, cytochrome P450, and oxidoreductase. Furthermore, quantitative real-time PCR (qPCR) was used to assess the expression levels of some critical genes that were involved in immune response and fungal pathogenicity. The results showed that their expression levels were consistent with the transcriptomes. Taken together, our findings offered a comprehensive and precise transcriptome study to understand the immune defense in H. xiaojinensis and O. sinensis invasion, which would accelerate the large-scale artificial cultivation of this medicinal fungus.
Risk of Cardiac Lesion with Chronic and Acute Use of Loperamide—An Integrative Review
Authors: Lammoglia Bruna Cremonezi , Hasselmann Gabriela , Pires-Oliveira Marcelo , Duarte Nicolau Lucas Antonio , Rolim Medeiros Jand Venes , Sabia Tallo Fernando , Omar Taha Murched , Yamaguti Lima Rildo , Caricati-Neto Afonso , Menezes-Rodrigues Francisco Sandro
Published: December 2022

Loperamide is a synthetic opioid commonly used as an antidiarrheal due to its activation of u-opioid receptors in the myenteric plexus. In therapeutic doses, it inhibits peristalsis and has anti-secretory and anti-motility effects, until metabolized by intestinal and hepatic CYP3A4 and CYP2C8 into inactive metabolites. Furthermore, loperamide also inhibits L-type voltage-gated calcium (Ca2+) channels, increases action potential duration, and can induce arrhythmias and even cardiotoxicity, particularly when taken in extremely high doses. Thus, the aim of this study was to perform an integrative review of the available evidence in the recent literature on the cardiac risks of acute and chronic use of loperamide. In electrocardiogram (ECG) analysis, the most common finding was QTc prolongation in 27 cases, followed by QRS prolongation, first-degree atrioventricular (AV) block, torsades de pointes, ventricular tachycardia, and right bundle branch block. As for the symptoms encountered, syncope, weakness, palpitations, lightheadedness, shortness of breath, nausea, vomiting, bradycardia, and cardiac arrest were the most common. Loperamide can inhibit hERG voltage-gated potassium (K+) channels (Kv11.1), leading to the prolongation of repolarization, QTc interval prolongation, and increased risk of torsades de pointes. In addition, loperamide can inhibit voltage-gated sodium (Na+) channels (Nav1.5), impairing electrical cardiac conduction and potentiating QRS interval widening. Therefore, QTc prolongation, torsades de pointes, and other ECG alterations are of particular concern regarding loperamide toxicity, particularly when overdosed.
Potential Drug Targets for Ceramide Metabolism in Cardiovascular Disease
Authors: Guo Jiaying , Feng Jiling , Qu Huiyan , Xu Hongxi , Zhou Hua
Published: December 2022

Cardiovascular disease poses a significant threat to the quality of human life. Metabolic abnormalities caused by excessive caloric intake have been shown to lead to the development of cardiovascular diseases. Ceramides are structural molecules found in biological membranes; they are crucial for cell survival and lipid metabolism, as they maintain barrier function and membrane fluidity. Increasing evidence has demonstrated that ceramide has a strong correlation with cardiovascular disease progression. Nevertheless, it remains a challenge to develop sphingolipids as therapeutic targets to improve the prognosis of cardiovascular diseases. In this review, we summarize the three synthesis pathways of ceramide and other intermediates that are important in ceramide metabolism. Furthermore, mechanistic studies and therapeutic strategies, including clinical drugs and bioactive molecules based on these intermediates, are discussed.
Statins and the Risk of Gastric Cancer: A Systematic Review and Meta-Analysis
Authors: Su Chun-Hsien , Islam Md. Mohaimenul , Jia Guhua , Wu Chieh-Chen
Published: December 2022

Previous epidemiological studies have reported that the use of statins is associated with a decreased risk of gastric cancer, although the beneficial effects of statins on the reduction of gastric cancer remain unclear. Therefore, we conducted a systematic review and meta-analysis to investigate the association between the use of statins and the risk of gastric cancer. Electronic databases such as PubMed, EMBASE, Scopus, and Web of Science were searched between 1 January 2000 and 31 August 2022. Two authors used predefined selection criteria to independently screen all titles, abstracts, and potential full texts. Observational studies (cohort and case-control) or randomized control trials that assessed the association between statins and gastric cancer were included in the primary and secondary analyses. The pooled effect sizes were calculated using the random-effects model. The Meta-analysis of Observational Studies in Epidemiology (MOOSE) reporting guidelines were followed to conduct this study. The total sample size across the 20 included studies was 11,870,553. The use of statins was associated with a reduced risk of gastric cancer (RRadjusted: 0.72; 95%CI: 0.64–0.81, p < 0.001). However, the effect size of statin use on the risk of gastric cancer was lower in Asian studies compared to Western studies (RRAsian: 0.62; 95%CI: 0.53–0.73 vs. RRwestern: 0.88; 95%CI: 0.79–0.99). These findings suggest that the use of statins is associated with a reduced risk of gastric cancer. This reverse association was even stronger among Asian people than Western individuals.
Gain a Baby Lose a Tooth—Is There an Association between Periodontitis and Preterm Birth?
Authors: Bartha Valentin , Steinmacher Sahra , Wittlinger Rebekka , Boutin Sébastien , Pauluschke-Fröhlich Jan , von Ohle Christiane , Brucker Sara Yvonne , Bruckner Thomas , Wolff Diana
Published: December 2022

Preterm birth serves as one of the leading causes of neonatal mortality worldwide. The underlying mechanisms that contribute to preterm birth are not yet fully understood. However, an association between periodontitis and preterm birth has been proposed. The periodontal status and presence of periodontal pathogens in women with different birth outcomes have been previously examined. However, varying definitions of periodontitis and different microbiological methods make their interpretation challenging. The aim of this case-control study on women with and without preterm birth was to investigate their periodontal status using the current classification system for periodontal diseases. Moreover, differences in the periodontal microbiome of the study participants were investigated. Therefore, we collected data on oral and periodontal parameters in 77 puerperal women divided into two groups based on gestational age at delivery: 33 patients with preterm birth (PTB, <37 weeks) and 44 patients with term birth (TB, >37 weeks). These data included pocket probing depth (PPD), clinical attachment loss (CAL), bleeding on probing (BOP), gingival-bleeding index, DMFT index, and gynecologic and dental history. In addition, their oral microbiome was explored. Median CAL and percentage PPD ≥ 4 mm were significantly higher in the PTB group than in the TB group (p = 0.0128 and p = 0.047, respectively). Birth weight was significantly higher in periodontally healthy women than in those with gingivitis (p = 0.0078) or periodontitis (p = 0.0127). The periodontal microbiome differed significantly between groups. Our results are underlining the possible association between periodontitis and preterm delivery. Women with periodontitis had babies with significantly lower birth weights. The microbiome varied between the groups.
Prevalence of Human Papillomavirus Infection in the Female Partner of Infertile Couples Undergoing IVF/ICSI-ET and Subsequent Reproductive Outcomes
Authors: Wei Sanhua , Wang Kaili , Cheng Fang , Chang Zhenhua , Ren Xiaoyan , Liu Zheng , Liu Mengxin , Yang Tao , Ma Xuhui , Xie Xiaojuan , Wang Xiaohong
Published: December 2022

We investigated the prevalence of human papillomavirus (HPV) infection in the female partner of infertile couples and the reproductive outcomes after in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET). We conducted a retrospective analysis on 8117 women from infertile couples who underwent IVF/ICSI treatment and evaluated the prevalence of HPV infection in these women. The prevalence of HPV infection in the female partner of infertile couples was 9.2% (747/8117). These HPV-infected female patients undergoing ART were divided into high-risk HPV (hrHPV) (n = 130) and low-risk HPV (lrHPV) groups (n = 94), and non-infected women patients formed the negative group (n = 126). Of the 747 cases infected with HPV, 529 showed hrHPV infection (70.82%; primarily genotypes 16, 52, 53, 58, and 59); 175 exhibited lrHPV infection (23.43%; primarily genotypes 6, 43, 44, 55, 61, and 81); and 43 cases were co-infected with hrHPV and lrHPV (5.76%). Except for the Day-3 high-quality embryo rate, there were no differences in ovum maturation, fertilization, implantation, clinical pregnancy, live birth, or miscarriage rates between women infected with HPV and non-infected women (p > 0.05); however, we noted an increased miscarriage rate after logistic regression analyses (OR, 0.16; 95% CI, 0.03–0.84; p = 0.041). For single-male-factor-induced infertility in couples (smHPV), although we likewise observed no differences in ovum maturation, fertilization, or implantation rates (p > 0.05) between the smHPV group and the negative group, we discerned diminutions in the Day-3 high-quality embryo rate (46.01% vs. 70.04%, p = 0.013), clinical pregnancy rate (46.67% vs. 57.94%, p = 0.003), and live birth rate (33.33% vs. 46.83%, p = 0.027) as well as an augmented miscarriage rate (11.11% vs. 4.76%, p = 0.003), respectively. Logistic regression analyses indicated that smHPV was a risk factor for decreased clinical pregnancy rate (OR, 4.17; 95% CI, 2.31–7.53; p < 0.001) and live birth rate (OR, 1.83; 95% CI, 0.81–2.14; p = 0.045) and elevated miscarriage rate (OR, 6.83; 95% CI, 2.22–21.00; p = 0.001). HPV infection in women was associated with increased miscarriage rate, and single-male-factor infertility influenced reproductive outcomes in couples undergoing IVF/ICSI treatment. Both were potentially due to HPV infection in the couple.
Acute Improvements of Oxygenation with Cpap and Clinical Outcomes in Severe COVID-19 Pneumonia: A Multicenter, Retrospective Study
Authors: Pini Stefano , Radovanovic Dejan , Saad Marina , Gatti Marina , Danzo Fiammetta , Mondoni Michele , Aliberti Stefano , Centanni Stefano , Blasi Francesco , Chiumello Davide Alberto , Santus Pierachille
Published: December 2022

It is not known if the degrees of improvement in oxygenation obtained by CPAP can predict clinical outcomes in patients with COVID-19 pneumonia. This was a retrospective study conducted on patients with severe COVID-19 pneumonia treated with CPAP in three University hospitals in Milan, Italy, from March 2020 to March 2021. Arterial gas analysis was obtained before and 1 h after starting CPAP. CPAP failure included either death in the respiratory units while on CPAP or the need for intubation. Two hundred and eleven patients (mean age 64 years, 74% males) were included. Baseline median PaO2, PaO2/FiO2 ratio (P/F), and the alveolar-arterial (A-a) O2 gradient were 68 (57–83) mmHg, 129 (91–179) mmHg and 310 (177–559) mmHg, respectively. Forty-two (19.9%) patients died in the respiratory units and 51 (24.2%) were intubated. After starting CPAP, PaO2/FiO2 increased by 57 (12–113; p < 0.001) mmHg, and (A-a) O2 was reduced by 68 (−25–250; p < 0.001) mmHg. A substantial overlap of PaO2, P/F, and A-a gradient at baseline and during CPAP was observed in CPAP failures and successes; CPAP-associated improvements in oxygenation in both groups were similar. In conclusion, CPAP-associated improvements in oxygenation do not predict clinical outcomes in patients with severe COVID-19 pneumonia.