bluuebunny/crossref_metadata_2025 huggingface dataset:
An easy and sensitive assay for acetohydroxyacid synthases based on the simultaneous detection of substrates and products in a single step
Authors: Engelhardt Annika , Ebeling Marco , Kaltenegger Elisabeth , Langel Dorothee , Ober Dietrich
Published: October 2024

Acetohydroxyacid synthase (AHAS, EC 2.2.1.6) catalyzes the first step in the synthesis of the branched-chain amino acids valine, leucine, and isoleucine, pathways being present in plants and microorganisms, but not in animals. Thus, AHAS is an important target for numerous herbicides and, more recently, for the development of antimicrobial agents. The need to develop new and optimized herbicides and pharmaceuticals requires a detailed understanding of the biochemistry of AHAS. AHAS transfers an activated two-carbon moiety derived from pyruvate to either pyruvate or 2-oxobutyrate as acceptor substrates, forming 2-acetolactate or 2-acetohydroxy-2-butyrate, respectively. Various methods have been described in the literature to biochemically characterize AHAS with respect to substrate preferences, substrate specificity, or kinetic parameters. However, the simultaneous detection and quantification of substrates and unstable products of the AHAS-catalyzed reaction have always been a challenge. Using AHAS isoform II from Escherichia coli, we have developed a sensitive assay for AHAS-catalyzed reactions that uses derivatization with ethyl chloroformate to stabilize and volatilize all reactants in the aqueous solution and detect them by gas chromatography coupled to flame ionization detection or mass spectrometry. This assay allows us to characterize the product formation in reactions in single and dual substrate reactions and the substrate specificity of AHAS, and to reinterpret previous biochemical observations. This assay is not limited to the AHAS-catalyzed reactions, but should be applicable to studies of many metabolic pathways.

Graphical Abstract
Influence of Ethnicity and Deprivation on Occurrence of Paget’S Disease in Greater Manchester, UK
Authors: Heald A. H. , Lu W. , Williams R. , McCay K. , Maharani A. , Cook M. J. , O’Neill T. W.
Published: October 2024

There is important variation in the occurrence of Paget’s disease in different regions and populations. There are though few data concerning the occurrence of clinically diagnosed disease in black and ethnic minority groups in the United Kingdom (UK). We undertook an anonymised search using an integrated primary and secondary care-based database in Greater Manchester, covering a population of over 3 million people. We looked also among those with a first positive COVID test, the influence of Paget’s disease on subsequent admission to hospital within 28 days. Within our database, there were 534,571 people aged 60 years and over alive on 1 January 2020. The majority were white (84%) with 4.7% identifying as Asian or Asian British, and 1.27% Black or Black British. There were 931 with clinically diagnosed Paget’s disease. Overall prevalence in the greater Manchester area was 0.174%. Prevalence was higher in men than women (0.195 vs 0.155%). Compared to the prevalence of Paget’s in whites (0.179%) the prevalence was lower among those identifying as Asian or Asian British (0.048%) and higher among those identifying as Black or Black British (0.344%). Prevalence increased with increasing deprivation. Clinically diagnosed Paget’s disease is uncommon affecting 0.174% of men and women aged 60 or more years. Within Greater Manchester, it was more common in those identifying as Black or Black British and less common in those identifying as Asian or Asian British.
Mechanical strength of the rotator cuff and cable interface: a complete histological and biomechanical study
Authors: Fondin Maxime , Miroir Mathieu , Guillin Raphaël , Landreau Julien , Ghukasyan Gevorg , Fautrel Alain , Ropars Mickael , Morandi Xavier , Nyangoh Timoh Krystel , Le Cam Jean-Benoît
Published: October 2024

Purpose

This study sought to evaluate the biomechanical properties of the interface between the rotator cuff and the semicircular humeral ligament or rotator cable (RCa) using histological and biomechanical techniques.

Methods

Out of 13 eligible cadaver specimens, 5 cadaver shoulders with an intact rotator cuff were included, 8 were excluded due to an injured rotator cuff. The histological study enables us to describe the capsule-tendon interface between the infraspinatus tendon (IST) or supraspinatus tendon (SST) and RCa, and to detect loose connective tissue layers to determine their precise location and measure their length along the interface. The biomechanical study sought to characterize and compare the mechanical strength of the IST-RCa versus SST-RCa interfaces.

Results

The average thickness of the RCa was 1.44 ± 0.20 mm. The histological study revealed a loose connective tissue layer at the IST-RCa interface, a finding not observed at the SST-RCa interface. The biomechanical study showed that the rigidity of the SST-RCa interface (72.10–2 N/mm) was 4.5 times higher than for the IST-RCa interface (16.10–2 N/mm) and the average maximum forces reached were 19.0 N and 10.6 N for the SST-RCa and IST- RCa interfaces, respectively.

Conclusion

The IST-RCa interface consists of a loose connective tissue layer contrary to the SST-RCa interface. In parallel, two different groups in terms of the mechanical response were identified: the IST-RCa interface group had less rigidity and ruptured more quickly than the SST-RCa interface, therefore emerging as the most vulnerable interface and explaining a potential extension of rotator cuff tears.
Long-term safety and efficacy of endovascular ultrasound renal denervation in resistant hypertension: 8-year results from the ACHIEVE study
Authors: Zeijen Victor J. M. , Völz Sebastian , Zeller Thomas , Mahfoud Felix , Kunz Michael , Kuck Karl-Heinz , Andersson Bert , Graf Tobias , Sievert Horst , Kahlert Philipp , Horesh-Bar Meital , Lenzen Mattie J. , Kardys Isabella , Daemen Joost
Published: October 2024

Background

Ultrasound renal sympathetic denervation (uRDN) reduces blood pressure (BP) in the absence and presence of antihypertensive treatment at 2 months. Beyond 3 years, there is a lack of follow-up data. This study investigated the long-term safety and efficacy of uRDN.

Methods

This prospective observational study recruited patients previously included in the international multicenter ACHIEVE study, with office systolic blood pressure (SBP) ≥160 mmHg, 24 h ambulatory SBP ≥130 mmHg, ≥3 antihypertensive drugs and estimated Glomerular Filtration Rate (eGFR) ≥45 ml/min/1.73m2 undergoing uRDN. The primary efficacy outcome was 24 h ambulatory SBP, adjusted for the number of defined daily dosages (DDD) of antihypertensive drugs. Statistical analyses were performed using linear mixed-effects models and inverse probability weighting.

Results

A total of 27 out of the initially enrolled 96 patients underwent prospective follow-up at a median of 8.2 [7.6−8.9] years. Mean age was 62.6±9.3 years (37.0% female). Preprocedural 24 h ambulatory BP was 151.9/84.1±11.5/11.1 mmHg and the median number of DDDs was 5.0 [4.3−7.0]. At 8 years after uRDN, the change in 24 h ambulatory SBP was −19.5 [95%CI −26.7,−12.4] mmHg (p<0.001). The 8-year change in the number of DDDs was −1.7 [−2.8,−0.6] (p = 0.003). The 8-year decline in eGFR was −8.9 [−13.2,−4.7] ml/min/1.73m2 (p<0.001). Clinical event data were available for all 96 patients (median follow-up 3.5 [1.0–8.0] years). Renal failure occurred in one patient and no cases of renal artery stenosis were detected.

Conclusions

A significant BP reduction was observed up until 8 years following uRDN in parallel to a decrease in drug burden over time, in the absence of procedure-related adverse events.
Algorithms in acetabular fracture classifications
Authors: Gänsslen Axel , Tonetti Jerome , Pohlemann Tim
Published: October 2024

Acetabular fractures are still challenging fractures. Fracture classification is the basis for understanding these injuries and to gain treatment and to choose a fracture type-based treatment concept. Using a systematic step-wise concept of fracture analysis, based on specific radiographic lines on standard X-rays allow even less experienced surgeons to get a correct classification of the elementary and associated fracture types of Letournel’s classification. Algorithmic analysis of the iliopectineal line, (anterior column involvement), ilioischial line (posterior column involvement), presence of a posterior wall fragment, fracture lines involving the iliac wing and inferior ramus, and the spur sign (representing associated both column fractures) allow for approximately 80–90% correct classifications using standard X-rays when integrating these lines into an algorithm. Especially, T-shaped fractures, ABC and ACPHT fractures may be difficult to classify. Thus, advanced imaging, such as CT scans with multiplanar reconstruction and 3D reconstructions is additionally recommended.
Reporting of somatic variants in clinical cancer care: recommendations of the Swiss Society of Molecular Pathology
Authors: Christinat Yann , Hamelin Baptiste , Alborelli Ilaria , Angelino Paolo , Barbié Valérie , Bisig Bettina , Dawson Heather , Frattini Milo , Grob Tobias , Jochum Wolfram , Nienhold Ronny , McKee Thomas , Matter Matthias , Missiaglia Edoardo , Molinari Francesca , Rothschild Sacha , Sobottka-Brillout Anna Bettina , Vassella Erik , Zoche Martin , Mertz Kirsten D.
Published: October 2024

Somatic variant testing through next-generation sequencing (NGS) is well integrated into Swiss molecular pathology laboratories and has become a standard diagnostic method for numerous indications in cancer patient care. Currently, there is a wide variation in reporting practices within our country, and as patients move between different hospitals, it is increasingly necessary to standardize NGS reports to ease their reinterpretation. Additionally, as many different stakeholders—oncologists, hematologists, geneticists, pathologists, and patients—have access to the NGS report, it needs to contain comprehensive and detailed information in order to answer the questions of experts and avoid misinterpretation by non-experts. In 2017, the Swiss Institute of Bioinformatics conducted a survey to assess the differences in NGS reporting practices across ten pathology institutes in Switzerland. The survey examined 68 reporting items and identified 48 discrepancies. Based on these findings, the Swiss Society of Molecular Pathology initiated a Delphi method to reach a consensus on a set of recommendations for NGS reporting. Reports should include clinical information about the patient and the diagnosis, technical details about the sample and the test performed, and a list of all clinically relevant variants and variants of uncertain significance. In the absence of a consensus on an actionability scheme, the five-class pathogenicity scheme proposed by the ACMG/AMP guideline must be included in the reports. The Swiss Society of Molecular Pathology recognizes the importance of including clinical actionability in the report and calls on the European community of molecular pathologists and oncologists to reach a consensus on this issue.
Possibility of determining high quantitative fecal occult blood on stool surface using hyperspectral imaging
Authors: Ikematsu Hiroaki , Takara Yohei , Nishihara Keiichiro , Kano Yuki , Owaki Yuji , Okamoto Ryuji , Fujiwara Takahisa , Takamatsu Toshihiro , Yamada Masayuki , Tomioka Yutaka , Takeshita Nobuyoshi , Inaba Atsushi , Sunakawa Hironori , Nakajo Keiichiro , Murano Tatsuro , Kadota Tomohiro , Shinmura Kensuke , Koga Yoshikatsu , Yano Tomonori
Published: October 2024

Background

Fecal immunochemical tests are commonly performed for colorectal cancer screening. Instant fecal occult blood measurement in toilet bowel movements would improve convenience. Hyperspectral imaging (HSI) enables the nondestructive evaluation of materials that are difficult to assess visually. This study aimed to determine whether HSI could be used to identify fecal occult blood on stool surfaces.

Methods

The study included 100 patients who underwent colonoscopy, divided into groups A and B (50 patients, each) for creating a discriminant algorithm and validating the accuracy of the algorithm, respectively. In group A, 100 areas were randomly selected from the stool surface, and the fecal occult blood quantitative values were measured and photographed using a hyperspectral camera (cutoff: > 400 ng/mL). A discriminant algorithm image was created to extract spectral feature differences obtained from HSI via machine learning. In group B, 250 random areas were evaluated and compared to fecal occult blood quantitative values, measuring sensitivity, specificity, accuracy, positive predictive value (PPV), and negative predictive value (NPV).

Results

Groups A and B comprised 28 and 26 patients with cancer, respectively. Cancer detection sensitivity at the 400 ng/mL cutoff was 67.9% and 42.3% in groups A and B, respectively. The discriminant algorithm image exhibited high accuracy in group A (sensitivity; 77.1%, specificity; 96.9%, accuracy; 90.0%, PPV; 93.1%, NPV; 88.7%). In group B, the sensitivity, specificity, accuracy, PPV, and NPV were 83.3, 92.9, 90.8, 76.3, and 95.3%, respectively.

Conclusion

HSI can effectively discriminate high quantitative fecal occult blood, highlighting its potential for improved colorectal cancer screening.
Temporary loop end ileostomy reduces the risk of stoma outlet obstruction: a comparative clinical study in patients undergoing restorative proctocolectomy and ileal pouch-anal anastomosis
Authors: Ogino Takayuki , Sekido Yuki , Mizushima Tsunekazu , Fujii Makoto , Mori Ryota , Takeda Mitsunobu , Hata Tsuyoshi , Hamabe Atsushi , Miyoshi Norikatsu , Uemura Mamoru , Doki Yuichiro , Eguchi Hidetoshi
Published: October 2024

Purpose

Stoma outlet obstruction (SOO) is a serious complication of restorative proctocolectomy (RPC) and ileal pouch-anal anastomosis (IPAA). When the ileal mesentery to the pouch is under excessive tension, the ileum near the ileostomy twists easily, causing SOO. Loop-end ileostomy (EI) for fecal diversion was introduced in 2021 to prevent SOO, and we aimed to verify whether temporary EI reduces the incidence of SOO in RPC and IPAA patients relative to loop ileostomy (LI).

Methods

This study included 106 consecutive RPC and IPAA patients with a diverting ileostomy and categorized them into LI (n = 75) or EI (n = 31) groups. The clinical characteristics of the patients were analyzed and compared.

Results

Patient characteristics were similar between the groups, except for higher preoperative steroid use in the LI group (38.7%; p = 0.0116). There were no significant differences between the groups in anatomical factors, such as abdominal wall thickness and the height-adjusted distance between the root of the superior mesenteric artery and the bottom of the external anal sphincter. There were no significant differences in surgery-related factors, with ≥ 90% of the patients in each group undergoing laparoscopic procedures. A multivariate logistic regression analysis revealed that EI significantly reduced the risk of SOO relative to LI (OR, 0.18; 95% CI 0.03–0.92; p = 0.0399).

Conclusion

EI reduced SOO levels after RPC and IPAA and may be beneficial for cases in which anastomosis is challenging.
How mature is requirements engineering for AI-based systems? A systematic mapping study on practices, challenges, and future research directions
Authors: Habiba Umm-e- , Haug Markus , Bogner Justus , Wagner Stefan
Published: October 2024

Artificial intelligence (AI) permeates all fields of life, which resulted in new challenges in requirements engineering for artificial intelligence (RE4AI), e.g., the difficulty in specifying and validating requirements for AI or considering new quality requirements due to emerging ethical implications. It is currently unclear if existing RE methods are sufficient or if new ones are needed to address these challenges. Therefore, our goal is to provide a comprehensive overview of RE4AI to researchers and practitioners. What has been achieved so far, i.e., what practices are available, and what research gaps and challenges still need to be addressed? To achieve this, we conducted a systematic mapping study combining query string search and extensive snowballing. The extracted data was aggregated, and results were synthesized using thematic analysis. Our selection process led to the inclusion of 126 primary studies. Existing RE4AI research focuses mainly on requirements analysis and elicitation, with most practices applied in these areas. Furthermore, we identified requirements specification, explainability, and the gap between machine learning engineers and end-users as the most prevalent challenges, along with a few others. Additionally, we proposed seven potential research directions to address these challenges. Practitioners can use our results to identify and select suitable RE methods for working on their AI-based systems, while researchers can build on the identified gaps and research directions to push the field forward.
Evaluating overhanging slope failure mechanisms and rockfall trajectories through integrated UAV imagery and discrete element modeling
Authors: Weng Meng-Chia , Zhang Geng-Man , Fu Yu-Yao , Le Hoang-Khanh , Kao Yu-Hui
Published: October 2024

To evaluate the threat of rockfall from an overhanging slope above a bridge, this study proposes a comprehensive analysis procedure to assess the failure mechanisms of the slope and the associated rockfall trajectories. The analysis utilizes high-resolution point cloud data obtained through integrated UAV-based imagery technology, enabling a detailed evaluation of joint configurations and slope stability. The geometric reconstruction of unstable rock blocks, combined with stereographic projections, provides an in-depth analysis of potential failure modes. Discrete element modeling (DEM) is employed to simulate the failure processes and predict rockfall trajectories, with particular emphasis on the potential risks to the underlying bridge. The results indicate that rockfall events near the bridge are primarily driven by wedge-shaped failures, a finding confirmed by both the proposed inverted three-dimensional projection method and DEM simulations. The analysis identifies critical rock blocks near the bridge, underscoring the need for targeted mitigation strategies. This case study demonstrates the effectiveness of integrating UAV-derived data with DEM for assessing overhanging slope failures and managing rockfall risks in similar geological settings.
Increasing loneliness among German children and adolescents from 2018 to 2022: A cross-sectional survey before and after the onset of the COVID-19 pandemic
Authors: Schütz Raphael , Bilz Ludwig
Published: October 2024

Aim

Loneliness is an increasingly present topic in scientific and public discourse, and has gained relevance due to the COVID-19 pandemic. Nevertheless, there is a lack of representative studies examining the prevalence of loneliness among children and adolescents before and after the pandemic. Accordingly, the present study aimed to determine the prevalence of loneliness among 11- to 15-year-old students before (2018) and after (2022) the onset of the COVID-19 pandemic and to investigate subgroup differences.

Subject and methods

In this cross-sectional Health Behaviour in School-aged Children (HBSC) study, the prevalence of loneliness was compared in two representative samples from the German federal state of Brandenburg from 2018 and 2022. In 2018, data were collected from 3,057 (51.8% girls, Mage = 13.1, SD = 1.7) and, in 2022, from 3,801 (52.6% girls, Mage = 13.5, SD = 1.6) students in general education schools. Loneliness was assessed using a single item from the Center for Epidemiologic Studies Depression Scale (CES-D). Chi-square test and logistic regressions were performed.

Results

In total, 11.8% of the students reported being lonely in 2018, and 19.3% reported being lonely in 2022. Loneliness was more prevalent among girls, nonbinary, older adolescents, and students with lower family affluence. The increase in loneliness did not differ depending on age, gender, or family affluence.

Conclusion

The findings highlight that loneliness among children and adolescents is a widespread phenomenon that has increased significantly from 2018 to 2022. There is a need for prevention measures and further research into health-related associations of loneliness.
Adsorption and evolution of N2 molecules over ZnO monolayer: a combined DFT and kinetic Monte-Carlo insight
Authors: Ghosh Sulagna , Nath Palash , Sanyal Dirtha
Published: October 2024

A large surface area, wide band gap, and unique bonding property between Zn and O atoms make the hexagonal ZnO monolayer attractive as a gas sensor. In the present work, the adsorption and evolution of nitrogen (N2) molecules over a ZnO monolayer have been studied using two different theoretical methods: van der Waals density functional theory (vdW-DFT) and kinetic Monte-Carlo (kMC) simulation. The adsorption and diffusion (hopping over the surface) energy of a N2 gas molecule has been calculated considering the different sites over the ZnO substrate using the revPBE-vdW functional. Bader charge, electron localization function analysis, density of states and band structure plotting have been used to understand the adsorption mechanism. Lateral repulsive interaction between two N2 molecules limits the maximum packing number of gas molecules within one hexagonal ring. The output of the vdW-DFT calculation has been fed to the kMC code to predict the rate of adsorption, desorption, and diffusion, along with the overall surface coverage at different temperatures and pressures. Finally, the change in the N2 adsorption energy has been predicted with the increase of the ZnO layer number.
Predicting the potential habitat of bears under a changing climate in Nepal
Authors: Baral Rishi , Adhikari Binaya , Paudel Rajan Prasad , Kadariya Rabin , Subedi Naresh , Dhakal Bed Kumar , Shimozuru Michito , Tsubota Toshio
Published: October 2024

In Nepal, the distributions of three bear species vary: sloth bears (Melursus ursinus) in the lowlands, Asiatic black bears (Ursus thibetanus) in the mid-hills, and brown bears (Ursus arctos) in the high Himalayas. We utilized 179 occurrence points for sloth bears, 199 for Asiatic black bears, and 41 points for brown bears to construct a habitat model incorporating climate and topographic variables. Employing various species distribution modeling algorithms in BIOMOD2, the model predicts suitable habitats spanning 10,971.75 km2 for sloth bears; 29,470.75 km2 for Asiatic black bears; and 6152.97 km2 for brown bears. Within protected areas, the habitat for sloth bears is 4120.56 km2, that for Asiatic black bears is 9688.67 km2, and that for brown bears is 4538.67 km2. Chitwan National Park emerged as the prime sloth bear habitat with a core area of 918.55 km2 and a buffer zone of 726.485 km2. The Annapurna Conservation Area was deemed suitable for Asiatic black bears and brown bears, covering 2802.23 km2 and 2795.91 km2, respectively. The models projected a significant reduction in the habitat of these bear species both inside and outside protected areas. As predicted under the Shared Socioeconomic Pathways (SSP)2–4.5 scenario, sloth bears may experience 54.9% (2050) and 44.7% (2070) losses, respectively, of habitat; Asiatic black bears, 11.2% (2050) and 16.8% (2070); and brown bears, 68.41% (2050) and 82.20% (2070) losses. The overlap between sloth bears and black bears spans 38.7 km2, and that between brown bears and black bears is 26.6 km2. Notably, all three bear species exhibited suitability correlations with the intermediate temperature of the driest quarter. Examining current and projected habitats provides essential information for guiding conservation strategies and ensuring the conservation of these bear species in the face of climate change.
English fronting constructions as a window to the semantics of tense: the case of belief reports
Authors: Kusliy Petr
Published: October 2024

This paper delves into the temporal interpretation of fronting constructions in English, a topic that has received limited attention in the literature on tense semantics. It presents new empirical findings revealing that specific fronting configurations, involving present tense morphology in a complement CP under a matrix past tense, can yield a theoretically unexpected simultaneous interpretation. A novel theoretical framework for understanding English tense is proposed, which accounts for the temporal interpretation of both fronting and non-fronting versions of attitude reports. The framework introduces a null simultaneous tense as a replacement for the conventional indexical present and the Sequence-of-Tense (SOT) de se tense. It is argued that in complement CPs the null simultaneous tense, as well as past tense, can be anchored either to the attitude time or to the utterance time. In either case, each tense receives a de re interpretation. Moreover, the paper contends that evidence from fronting constructions supports Kratzer’s (Proceedings of semantics and linguistic theory 8, pp. 92–110, 1998) proposal that the transmission of temporal features (as part of the mechanism of SOT) occurs at PF. Finally, the proposed revision of the English tense system contributes to a more unified cross-linguistic perspective on tense. It is demonstrated how the temporal readings of attitude reports arising in such non-SOT languages as Russian, Japanese, and Hebrew can be captured within the system developed for English, with known contrasts attributed to the absence of Feature Transmission in those languages. Further known and new data from English, Modern Greek, and German are examined, revealing variations in SOT effects across SOT languages. These data are used as additional evidence supporting the claim that cross-linguistic variation in the interpretation of complement tense stems solely from differences in Feature Transmission. Regarding the semantic profile of tenses, it can be considered largely invariant.
Wine industry by-products as a source of active ingredients for topical applications
Authors: Gonçalves Maria Beatriz S. , Marques Mário Pedro , Correia Fernando , Pires Patrícia C. , Correia Mafalda , Makvandi Pooyan , Varela Carla , Cefali Letícia Caramori , Mazzola Priscila Gava , Veiga Francisco , Cabral Célia , Mascarenhas-Melo Filipa , Paiva-Santos Ana Cláudia
Published: October 2024

The wine industry is responsible for a considerable amount of waste produced by agricultural activities worldwide, which impacts both the economic sector and the environment through pollution and ecosystem imbalance. There are several examples of by-products generated during the winemaking process, such as grape pomace, vine leaves, grape stalks, or wine lees. These can be recovered and reused by the cosmetic industry, which is actively seeking natural and greener products. There are active ingredients that come from by-products that have relevant bioactive properties such as antioxidant, antiaging, anti-hyperpigmentation, or cellular protection. Therefore, the use of by-products from the wine industry as a source of active ingredients for topical application has attracted attention from the scientific community, overcoming some of the drawbacks of the winemaking process. Further research into extraction processes and methods is essential, as they directly influence the quality and quantity of recovered active ingredients. While current cosmetic products on the market undergo safety and cytotoxicity testing, it is important to evaluate the effects of pesticide treatments applied before pruning and the toxicity of the by-products to ensure the safety of future formulations. This review addresses a critical discussion on this subject, assessing the potential benefits of their use in topical formulations.

Graphical Abstract
Administration of melatonin nanoparticles improves testicular blood flow, echotexture of testicular parenchyma, scrotal circumference, and levels of estradiol and nitric oxide in prepubertal ossimi rams under summer heat stress
Authors: Fayez Eman , Samir Haney , Youssef Fady Sayed , Salama Ali , ElSayed Mohamed AI
Published: October 2024

Environmental heat stress (HS) impairs reproductive efficiency in farm animals. This study investigated, for the first time, how the melatonin and melatonin nanoparticles treatment affected the testicular hemodynamics, testicular volume, echotexture [Pixel intensity (PIX) and integrated density (IND)], scrotal circumference, serum concentration of testosterone (T), estradiol (E2), nitric oxide (NO), and total antioxidant capacity (TAC) in prepubertal Ossimi ram lambs in hot climatic conditions. The lambs undergoing examination had a temperature humidity index (THI) of 87.05 ± 1.70, indicating severe HS condition. Fifteen prepubertal Ossimi ram lambs were exposed to a single s.c injection of either nano melatonin (nano melatonin group; 20 mg/ram; n = 5) or melatonin suspended in two ml of corn oil (melatonin group; 40 mg/ram; n = 5) or two ml of corn oil (control group; n = 5). Blood collection and ultrasonographic assessment of the testes and supratesticular arteries (STAs) were conducted immediately before treatment (W0) and once weekly for six successive weeks after nano melatonin and melatonin injection (W1-W6). Results revealed decreases (P < 0.05) in the Doppler indices (resistive index; RI and pulsatility index; PI) of the testicular arteries at most time points of the study in the nano melatonin and melatonin groups. PIX of testicular parenchyma was significantly increased (P ˂ 0.05) in the treated groups compared to the control one. IND of testicular parenchyma increased significantly in the nano melatonin group compared to the melatonin and control groups. Testicular volume and scrotal circumference significantly increased (P < 0.05) in nano melatonin and melatonin groups compared to the control one. T concentration did not significantly (P > 0.05) change in the treated groups compared to the control group. E2, NO, and TAC concentrations increased (P < 0.05) in the treated groups compared to the control one. In conclusion, this study extrapolated that administrations of melatonin or nano melatonin can ameliorate the effects of environmental HS in prepubertal Ossimi ram lambs with a more protective effect and lower dose of nano melatonin.
Application of intuitionistic fuzzy set in social life cycle impact assessment
Authors: Tokede Olubukola
Published: October 2024

Purpose

Intuitionistic fuzzy set (IFS) has been found promising for handling ambiguity forms of uncertainty in social data. This paper undertakes an application of an intuitionistic fuzzy set in the social life cycle impact assessment (S-LCIA) of a public building project. This work proposes to combine an intuitionistic fuzzy set (IFS) with multi-attribute decision-making in converting qualitative data into quantitative social impacts of the building project. This research utilises IFS to accommodate and handle experts’ imprecise cognitions in S-LCIA to facilitate the identification and prioritisation of the most pressing indicators accompanying social impacts in a project.

Methods

Data were collected using questionnaire(s), structured interviews, and through targeted focus group sessions. Intuitionistic fuzzy set (IFS) approach was used to model the obtained data from structured interviews. Using IFS, this research also accounts for missing or ambiguous data that emerged during the data elicitation process. In combining IFS with multi-attribute decision-making techniques, the social impacts of selected stakeholders were evaluated using fuzzy set and IFS approaches. Sensitivity analysis was then used to test the robustness of the results and ranking was conducted based on each social stakeholder subcategory.

Results and discussion

Results revealed that approximately 23% of missing datapoints in the public case study building were incorporated in the S-LCIA using IFS. The issues of highest priority in each of the considered subcategory in the public case study building project according to IFS are (i) consumer privacy by adopting more functional planning (0.27), (ii) public commitment to sustainability (0.33), and (iii) education provided in the local community by having an indigenous botanical element for children (0.39), respectively. Conversely, the FS technique inferred that the issue of highest priority in the consumer stakeholder is the feedback mechanism (0.26). The overall degree of correlation between the IFS and FS is only 0.234, revealing that IFS provides a different perspective from conventional FS when used in modelling social data.

Conclusion

The IFS method provides an objective and systematic approach for dealing with a heterogeneous scope of imprecise and inexact social data in achieving holistic social life cycle assessment results. Furthermore, expansive stakeholder involvement would rely on a robust approach for improving social well-being in public buildings, thereby leaving no one behind in accomplishing a sustainable world.
US Primary Care Workforce Growth: A Decade of Limited Progress, and Projected Needs Through 2040
Authors: Bazemore Andrew W. , Petterson Stephen M. , McCulloch Kade K.
Published: October 2024

Background

Despite efforts to mitigate a projected primary care physician (PCP) shortage required to meet an aging, growing, and increasingly insured population, shortages remain, compounded by the COVID-19 pandemic, growing inequity, and persistent underinvestment.

Objective

We examined primary care workforce trends over the past decade and revisited projected primary care clinician workforce needs through the year 2040.

Design and Participants

Using data from the AMA Masterfile and Medical Expenditure Panel Survey (MEPS), we analyzed trends in the number of primary care physicians (PCPs) and in outpatient PCP visits by age and gender over the past decade. We then used the Medicare PECOS and Physician & Other Practitioners datasets to identify nurse practitioners (NPs) and physician assistants (PAs) in primary care.

Measures

Using these baseline clinician enumerations and projected population growth estimates from the US Census Bureau for the years 2020–2040, we calculated estimated primary care workforce needs by 2040.

Key Results

The effects of aging and population growth and baseline shortages in the primary care workforce call for significant increases in the primary care workforce to accommodate rising demands. Office visits to primary care clinicians are projected to increase from 773,606 in 2020 to 893,098 in 2040. We project a need for an additional 57,559 primary care clinicians by 2040.

Conclusions

Workforce shortages in primary care continue to expand due to population aging, growth, and heightened rates of clinician burnout & egress.
On the Dimension of the Singular Set of Perimeter Minimizers in Spaces with a Two-Sided Bound on the Ricci Curvature
Authors: Cucinotta Alessandro , Fiorani Francesco
Published: October 2024

We show that the Hausdorff dimension of the singular set of perimeter minimizers in noncollapsed limits of manifolds with two-sided bounds on the Ricci curvature is at most

$$n-5$$

n
-
5

, where n is the dimension of the ambient space. The estimate is sharp.
Escalation of neoadjuvant therapy in triple-negative breast cancer—balancing effectiveness and toxicities
Authors: Suppan Christoph
Published: October 2024

With the incorporation of immune checkpoint inhibitors into the neoadjuvant treatment of patients with triple-negative breast cancer, we are confronted with a new toxicity profile, while adding significant improvement in pathologic complete response rates and event-free survival.
Lights on tardigrade biodiversity: integrative redescription of Eremobiotus alicatai (Eutardigrada, Isohypsibiidae) with new insights on its morphology, phylogeny, and biogeography
Authors: Camarda Daniele , Frigieri Federica , Guidetti Roberto , Cesari Michele , Lisi Oscar
Published: October 2024

Current knowledge on the genus Eremobiotus still remains limited. Only three species are known, mainly recorded in the Palaearctic region, with Eremobiotus alicatai representing the most common species. In the present study, an integrative re-description of E. alicatai based on the examination of a topotypic population from Sicily (Italy) is given, the morphological characters of which perfectly correspond to those of the type series collected in 1969. A second population of E. alicatai, from Tuscany (Italy), was also investigated from the morphological and the molecular point of view, allowing examining the intraspecific variability of the species. A re-analysis of the morphology of specimens of the type series, along with a morphological analysis of specimens of the two investigated populations of E. alicatai, allowed for an update and correction of certain morphological traits in the species. The topotypic and the Tuscanian populations were both investigated through a molecular approach: COI, ITS2, 18S, and 28S gene sequences were obtained, allowing to update the Isohypsibioidea phylogeny and to discuss the correct placement of the genus Eremobiotus. Moreover, the definition of the morphology of the claws of Eremobiotus-type and new observations regarding the buccal opening are discussed.
Distribution of tardigrade cryptobiotic abilities across a fine-scale habitat gradient
Authors: Stec Daniel , Vecchi Matteo , Budzik Krystian , Matsko Yelyzaveta , Miler Krzysztof
Published: October 2024

In this study, we examine the distribution of cryptobiotic abilities, specifically anhydrobiosis and cryobiosis, in tardigrade communities across a habitat gradient from the edge to the centre of a forest bordering a desert. Our study demonstrates no correlation between desiccation and freezing tolerance at the community level. However, communities inhabiting the forest centre show higher cryptobiotic performance, likely due to better energy reserves indicated by slower mortality rates during fasting. The observed distribution pattern of cryptobiotic abilities cannot be explained by differences in community compositions or body sizes, as these variables were uniform across the gradient. Our research highlights the significance of environmental factors in shaping cryptobiotic responses and sheds light on the functional characteristics underlying the distribution of tardigrades in fine-scale habitat gradients. Our findings also challenge the hypothesis of a correlation between desiccation and freezing tolerance in cryptobiotic organisms.
Ecological redesign of crop ecosystems for reliable crop protection. A review
Authors: Bommarco Riccardo
Published: October 2024

To attain food security, we must minimize crop losses caused by weed growth, animal herbivores, and pathogens (or “pests”). Today, crop production depends heavily on the use of chemical pesticides (or “pesticides”) to protect the crops. However, pesticides are phased out as they lose efficiency due to pest resistance, and few new pesticides are appearing on the market. In addition, policies and national action programs are implemented with the aim of reducing pesticide risks. We must redesign our cropping systems to successfully protect our crops against pests using fewer or no pesticides. In this review, I focus on the principles for redesigning the crop ecosystem. Ecological redesign aims to enhance ecological functions in order to regulate pest populations and diminish crop losses. Exploring ecology and ecosystems plays an important role in this transition. Guiding principles for redesigning the cropping system can be drawn from understanding its ecology. Ecosystem and community ecologists have identified four principal ecological characteristics that enhance the biotic regulation of ecological processes across ecosystems: (i) advanced ecosystem succession through introducing and conserving perennial crops and landscape habitats; (ii) reduced disturbance frequency and intensity; (iii) an increase in both managed and wild functional biological diversity, above and below ground; and (iv) matched spatial extent of land use (e.g., crop field size) with that of ecological processes (e.g., dispersal capacity of predators). I review the practices that link these ecosystem characteristics to crop protection in grain commodity cropping in both the crop field and the agricultural landscape. The review brings forth how basic understandings drawn from ecosystem and community ecology can guide agricultural research in the redesign of cropping systems, ensuring that technologies, breeding, innovation, and policy are adapted to and support the reshaped crop ecosystem.
Life cycle assessment in additive manufacturing of copper alloys—comparison between laser and electron beam
Authors: Raute Julius , Beret Alexander , Biegler Max , Rethmeier Michael
Published: October 2024

Additive manufacturing is becoming increasingly important for industrial production. In this context, directed energy deposition processes are in demand to achieve high deposition rates. In addition to the well-known laser-based processes, the electron beam has also reached industrial market maturity. The wire electron beam additive manufacturing offers advantages in the processing of copper materials, for example. In the literature, the higher energy efficiency and the resulting improvement in the carbon footprint of the electron beam are highlighted. However, there is a lack of practical studies with measurement data to quantify the potential of the technology. In this work, a comparative life cycle assessment between wire electron beam additive manufacturing (DED-EB) and laser powder additive manufacturing (DED-LB) is carried out. This involves determining the resources for manufacturing, producing a test component using both processes, and measuring the entire energy consumption. The environmental impact is then estimated using the factors global warming potential (GWP100), photochemical ozone creation potential (POCP), acidification potential (AP), and eutrophication potential (EP). It can be seen that wire electron beam additive manufacturing is characterized by a significantly lower energy requirement. In addition, the use of wire ensures greater resource efficiency, which leads to overall better life cycle assessment results.
Dynamic Regulation of Hydrogen Bonding Networks and Solvation Structures for Synergistic Solar-Thermal Desalination of Seawater and Catalytic Degradation of Organic Pollutants
Authors: Yu Ming-Yuan , Wu Jing , Yin Guang , Jiao Fan-Zhen , Yu Zhong-Zhen , Qu Jin
Published: October 2024

Although solar steam generation strategy is efficient in desalinating seawater, it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic pollutants. Herein, dynamic regulations of hydrogen bonding networks and solvation structures are realized by designing an asymmetric bilayer membrane consisting of a bacterial cellulose/carbon nanotube/Co2(OH)2CO3 nanorod top layer and a bacterial cellulose/Co2(OH)2CO3 nanorod (BCH) bottom layer. Crucially, the hydrogen bonding networks inside the membrane can be tuned by the rich surface –OH groups of the bacterial cellulose and Co2(OH)2CO3 as well as the ions and radicals in situ generated during the catalysis process. Moreover, both SO42− and HSO5− can regulate the solvation structure of Na+ and be adsorbed more preferentially on the evaporation surface than Cl−, thus hindering the de-solvation of the solvated Na+ and subsequent nucleation/growth of NaCl. Furthermore, the heat generated by the solar-thermal energy conversion can accelerate the reaction kinetics and enhance the catalytic degradation efficiency. This work provides a flow-bed water purification system with an asymmetric solar-thermal and catalytic membrane for synergistic solar thermal desalination of seawater/brine and catalytic degradation of organic pollutants.
Preparation of nano Cu-Mo2C interface supported on ordered mesoporous biochar of ultrahigh surface area for reverse water gas shift reaction
Authors: Pan Xueyuan , Sun Hao , Ma Mingzhe , Liao Haiquan , Zhan Guowu , Wang Kui , Fan Mengmeng , Xu Jingcheng , Ding Linfei , Sun Kang , Jiang Jianchun
Published: October 2024

High conversion rate and selectivity are challenges for CO2 utilization through catalytic reverse water gas shift (RWGS) reaction. Herein, a novel mesoporous biochar (MB) supported Cu-Mo2C nano-interface was prepared by consecutive physical activation of coconut shells followed by carbothermal hydrogen reduction of bimetal. As compared with traditional carbon materials, this MB exhibited ultra-high specific surface area (2693 m2 g–1) and mesopore volume of mesopore (0.81 cm3 g–1) with a narrow distribution (2–5 nm), responsible for the high dispersion of binary Cu-Mo2C sites, CO2 adsorption and mass transfer in the reaction system. Moderate carbothermal reduction led to the sufficient reduction of Mo ion with carbon matrix of MB and dispersive growth of nano Cu-Mo2C binary sites (~ 6.1 nm) on the surface of MB. Cu+ species were formed from Cu0 via electron transfer and showed high dispersion with simultaneous boosted bimetal loading due to the strong interaction between nano Mo2C and Cu. These were advantageous to the intrinsic activity and stability of the Cu-Mo2C binary sites and their accessibility to the reactant molecules. Under the RWGS reaction conditions of 500 °C, atmospheric pressure, and 300,000 ml/g/h gas hour space velocity, the CO2 conversion rate over Cu-Mo2C/MB reached 27.74 × 10–5 molCO2/gcat/s at very low H2 partial pressure, which was more than twice that over traditional carbon supported Cu-Mo2C catalysts. In addition, this catalyst exhibited 99.08% CO selectivity and high stability for more than 50 h without a decrease in activity and selectivity. This study offers a new development strategy and a promising candidate for industrial RWGS.

Graphical Abstract
Immunohistochemical Analysis of GATA2 Expression in Endometrium and its Relationship with Hormone Receptor Expression in Benign and Premalignant Endometrial Disorders
Authors: Keske Aysenur , Polaki Usha S. , Matson Daniel R.
Published: October 2024

The GATA gene family encodes highly conserved zinc-finger transcription factors that facilitate the development and function of multiple organ systems including the uterus. In the endometrium, GATA2 functions in a positive autoregulatory loop with the progesterone receptor (PGR) and colocalizes with PGR on chromatin to promote PGR transcriptional programs. GATA2 also has PGR-independent functions that maintain endometrial cell identity, and GATA2 transcripts reportedly are down-regulated in endometrial disorders including endometriosis. This event is accompanied by a reciprocal increase in GATA6. Here, we applied custom anti-GATA2 monoclonal antibodies and performed GATA2 immunohistochemistry (IHC) on patient endometrial tissues corresponding to proliferative, secretory, inactive, and hormone-treated endometrium, as well as endometriosis and endometrial atypical hyperplasia/endometrioid intraepithelial neoplasia (EAH/EIN). We also performed IHC for the estrogen receptor, PGR, and GATA6 in relevant groups. The results reveal a tight correlation between GATA2 and PGR expression in the glandular and stromal cells of benign endometrium. GATA2 expression is markedly reduced in stromal but not glandular cells in endometriosis and EAH/EIN. This reduction in GATA2 expression does not lead to a detectable increase in GATA6 expression in endometriosis. Although average glandular GATA2 expression was preserved in endometriosis and EAH/EIN cases, its expression was decoupled from PGR, implying that alternative pathways regulate GATA2 levels in these disorders. Our findings indicate that GATA2 dysregulation is a feature of endometriosis and EAH/EIN, and support a model whereby loss of stromal GATA2 in these disorders contributes to their progesterone insensitivity.