bluuebunny/crossref_metadata_2025 huggingface dataset:
Postoperative Changes in Systemic Immune Tolerance Following Major Oncologic versus Minor Maxillofacial Surgery
Authors: Trumet Leah , Ries Jutta , Sobl Philip , Ivenz Niclas , Wehrhan Falk , Lutz Rainer , Kesting Marco , Weber Manuel
Published: July 2023

Background: There is increasing evidence of the benefits of adjuvant and neoadjuvant immunotherapy in the treatment of solid malignancies like oral squamous cell carcinoma (OSCC). To optimize (neo-)adjuvant treatment, the systemic immunomodulatory effects of tumor surgery itself need to be considered. Currently, there is little evidence on the immunological effects of major surgery, such as free microvascular flap reconstruction. The current study aims to analyze how and to what extent maxillofacial surgery affects systemic parameters of immune tolerance. Methods: A total of 50 peripheral whole blood samples from patients (Group 1 (G1) = extensive OSCC surgery; Group 2 (G2) = free flap reconstruction without persistent malignant disease; Group 3 (G3) = minor maxillofacial surgery) undergoing surgery were included for real-time quantitative polymerase chain reaction (RT-qPCR) to examine changes in mRNA expression of the biomarkers IL-6, IL-10, FOXP3, and PD-L1. Blood samples were taken immediately before and after surgery as well as on the second, fourth, and tenth postoperative days. Differences in mRNA expression between groups and time points were calculated using statistical tests, including Mann–Whitney U-test and Pearson correlation analysis. Results: Comparing postoperative expression of G1 and G3, there was a significantly higher PD-L1 expression (p = 0.015) in G1 compared to G3 and a significantly lower IL-6 (p = 0.001) and FOXP3 (p = 0.016) expression. Interestingly, IL-10 expression was higher pre- (0.05) and postoperative (p < 0.001) in G1 compared to G3. Additionally, in G1, there was a significant overexpression of IL-10 post-surgery compared to the preoperative value (p = 0.03) and a downregulated expression of FOXP3 between pre- and 2 d post-surgery (p = 0.04). Furthermore, there was a significant correlation between the duration of surgery and the perioperative expression changes of the analyzed biomarkers. As the duration of surgery increased, the expression of IL-10 and PD-L1 increased, and the expression of IL-6 and FOXP3 decreased. Conclusion: Extensive surgery in OSCC patients is associated with a transient shift toward postoperative systemic immune tolerance compared with patients undergoing minor surgery. However, even extensive surgery causes no signs of long-lasting systemic immunosuppression. The degree of immune tolerance that occurred was associated with the duration of surgery. This supports efforts to minimize the duration of surgery.
Models in Pancreatic Neuroendocrine Neoplasms: Current Perspectives and Future Directions
Authors: Forsythe Steven D. , Pu Tracey , Andrews Stephen G. , Madigan James P. , Sadowski Samira M.
Published: July 2023

Pancreatic neuroendocrine neoplasms (pNENs) are a heterogeneous group of tumors derived from multiple neuroendocrine origin cell subtypes. Incidence rates for pNENs have steadily risen over the last decade, and outcomes continue to vary widely due to inability to properly screen. These tumors encompass a wide range of functional and non-functional subtypes, with their rarity and slow growth making therapeutic development difficult as most clinically used therapeutics are derived from retrospective analyses. Improved molecular understanding of these cancers has increased our knowledge of the tumor biology for pNENs. Despite these advances in our understanding of pNENs, there remains a dearth of models for further investigation. In this review, we will cover the current field of pNEN models, which include established cell lines, animal models such as mice and zebrafish, and three-dimensional (3D) cell models, and compare their uses in modeling various disease aspects. While no study model is a complete representation of pNEN biology, each has advantages which allow for new scientific understanding of these rare tumors. Future efforts and advancements in technology will continue to create new options in modeling these cancers.
Electrochemical Multiplexed N-Terminal Natriuretic Peptide and Cortisol Detection in Human Artificial Saliva: Heart Failure Biomedical Application
Authors: Ghedir El Kahina , Baraket Abdoullatif , Benounis Messaoud , Zine Nadia , Errachid Abdelhamid
Published: July 2023

The early detection at low concentration, by non-invasive methods, of cardiac biomarkers in physiological fluids has attracted the interest of researchers over the last decade. This enables early diagnosis and prediction of the first signs of heart failure (HF). In this respect, the analysis of human saliva remains the most suitable medium for this non-invasive approach, as it contains a highly interesting biological matrix for general health and disease monitoring. In this work, we developed a highly sensitive multiplexed immunosensor for direct simultaneous detection of both N-terminal Natriuretic Peptide (NT-proBNP) and Cortisol in human artificial saliva (AS). The developed biosensor platform based on silicon nitride substrate was composed from four gold working microelectrodes (WEs) and an integrated counter and reference microelectrode. Gold WEs were biofunctionalized through carboxyl diazonium (4-APA) to immobilize both anti-NT-proBNP and anti-Cortisol antibodies for simultaneous detection. The electroaddressing of the 4-APA onto the gold WE surfaces was realized with cyclic voltammetry (CV), while the interaction between antibodies and antigens in PBS was monitored using electrochemical impedance spectroscopy (EIS). The antigen detection in human AS was realized with EIS combined with the standard addition method. The immunosensor was highly sensitive and selective toward the corresponding biomarkers in both PBS and artificial human saliva as well as in the presence of other potential interfering biomarkers such as tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10). The limit of detection (LOD) was at 0.2 pg/mL for NT-proBNP within the range of 0.03 to 0.9 pg/mL, while the LOD for Cortisol was 0.06 ng/mL within the range of 0.02 to 0.6 ng/mL for Cortisol in artificial saliva. The developed immunosensor is very promising for significant detection in physiological media, and time reducing as it allows the simultaneous detection of various biomarkers.
Recent Advances in Design Strategies and Imaging Applications of Fluorescent Probes for ATP
Authors: Gu Qing-Song , Li Ting , Liu Ting , Yu Guo , Mao Guo-Jiang , Xu Fen , Li Chun-Yan
Published: July 2023

Adenosine 5′-triphosphate (ATP) is the energy currency in cells. It is involved in numerous cellular life activities and exhibits a close association with the development of certain diseases. Thus, the precise detection of ATP within cells holds immense significance in understanding cell biological events and related disease development. Fluorescent probes have obvious advantages in imaging ATP in cells and in vivo due to their high sensitivity, good selectivity, real-time imaging, and good biocompatibility. Thus far, an extensive array of fluorescent probes targeting ATP has been formulated to enable the visualization of ATP within cells and in vivo. This review summarizes the recent advances in ATP fluorescent probes according to different design strategies, mainly including those based on organic small molecules, metal complexes, and water-soluble conjugated polymers. In addition, the practical applications of ATP fluorescent probes in the imaging of target organelles, cell biological events, and disease markers are highlighted. Finally, the challenges and future trends of ATP detection based on fluorescent probes are discussed.
The Price of Success—The Long-Term Outcomes of Children with Craniopharyngioma—Two Institutions’ Experience
Authors: Napieralska Aleksandra , Mandera Marek , Sordyl Ryszard , Antosz Aleksandra , Bekman Barbara , Blamek Sławomir
Published: July 2023

An analysis of patients below 21 years old treated due to craniopharyngioma in the years 1979–2022 was performed with the aim of evaluating the long-term outcome and treatment side-effects. The standard statistical tests were used, and 56 patients with a median age of 11 years were evaluated. Surgery was the primary treatment in 55 patients; however, in only 29 it was the only neurosurgical intervention. Eighteen children were treated with radiotherapy (RTH) in primary treatment. The most common neurosurgical side effects observed were visual and endocrine deficits and obesity, which were diagnosed in 27 (49%), 50 (91%), and 25 (52%) patients, respectively. Complications after RTH were diagnosed in 14 cases (32%). During the median follow-up of 8.4 years (range: 0.4–39.8 years), six patients died and the 5- and 10-year overall survival was 97% and 93%, respectively. Five-year progression-free survival for gross total resection, resection with adjuvant RTH, and non-radical resection alone was 83%, 68%, and 23%, respectively (p = 0.0006). Surgery combined with RTH provides comparable results to gross tumor resection in terms of oncologic outcome in craniopharyngioma patients. Adjuvant irradiation applied in primary or salvage treatment improves disease control. The rate of complications is high irrespective of improved surgical and radiotherapeutic management.
Latent Transitions of Learning Interests among Kindergarteners in Hakka Bilingual Teaching Programs
Authors: Wu Chung-Chin
Published: July 2023

The profiles of kindergarteners’ learning interests in Hakka bilingual teaching programs are unclear and the effects of these programs on the transition of such interests over the long term are under investigation. This study analyzed the learning interest profiles of 112 kindergarteners (data gathered by kindergarten teachers) enrolled in immersion/non-immersion Hakka bilingual programs in Taiwan. Latent transitions in these profiles were analyzed based on pre- and post-implementation data. The results showed that two different subgroups were identified based on the kindergarteners’ learning interest profiles before and after the implementation of the Hakka bilingual program. The pre-implementation subgroups contained the “moderate situational and low-to-moderate individual interest” and the “high situational and moderate-to-high individual interest” profiles. Post-implementation subgroups consisting of “moderate-to-high situational and moderate individual interest” and “high situational and individual interest” profiles were identified. Moreover, four patterns of transition in the kindergarteners’ learning interest profiles were uncovered: (1) a slight increase in both learning interests, (2) a significant increase in both learning interests, (3) a slight regression in both, and (4) a maintenance of situational interest coupled with a slight increase in individual interest. Lastly, the non-immersion Hakka program showed significant and more positive effects on the “moderate situational and low-to-moderate individual interest” profile subgroup compared to the equivalent group from the Hakka immersion program. These results provide new evidence complementing previous findings reached via different analytical approaches and contribute to the overall conclusion that bilingual programs improve learning outcomes.
Kinetic Models of Wealth Distribution with Extreme Inequality: Numerical Study of Their Stability against Random Exchanges
Authors: Ghosh Asim , Banerjee Suchismita , Goswami Sanchari , Mitra Manipushpak , Chakrabarti Bikas K.
Published: July 2023

In view of some recent reports on global wealth inequality, where a small number (often a handful) of people own more wealth than 50% of the world’s population, we explored if kinetic exchange models of markets could ever capture features where a significant fraction of wealth can concentrate in the hands of a few as the market size N approaches infinity. One existing example of such a kinetic exchange model is the Chakraborti or Yard-Sale model; in the absence of tax redistribution, etc., all wealth ultimately condenses into the hands of a single individual (for any value of N), and the market dynamics stop. With tax redistribution, etc., steady-state dynamics are shown to have remarkable applicability in many cases in our extremely unequal world. We show that another kinetic exchange model (called the Banerjee model) has intriguing intrinsic dynamics, where only ten rich traders or agents possess about 99.98% of the total wealth in the steady state (without any tax, etc., like external manipulation) for any large N value. We will discuss the statistical features of this model using Monte Carlo simulations. We will also demonstrate that if each trader has a non-zero probability f of engaging in random exchanges, then these condensations of wealth (e.g., 100% in the hand of one agent in the Chakraborti model, or about 99.98% in the hands of ten agents in the Banerjee model) disappear in the large N limit. Moreover, due to the built-in possibility of random exchange dynamics in the earlier proposed Goswami–Sen model, where the exchange probability decreases with the inverse power of the wealth difference between trading pairs, one does not see any wealth condensation phenomena. In this paper, we explore these aspects of statistics of these intriguing models.
Friction and Stiffness Dependent Dynamics of Accumulation Landslides with Delayed Failure
Authors: Kostić Srđan , Todorović Kristina , Lazarević Žarko , Prekrat Dragan
Published: July 2023

We propose a new model for landslide dynamics under the assumption of a delay failure mechanism. Delay failure is simulated as a delayed interaction between adjacent blocks, which mimics the relationship between the accumulation and feeder part of the accumulation slope. The conducted research consisted of three phases. Firstly, the real observed movements of the landslide were examined to exclude the existence or the statistically significant presence of background noise. Secondly, we propose a new mechanical model of an accumulation landslide dynamics, with introduced delay failure, and with variable friction law. Results obtained indicate the onset of a transition from an equilibrium state to an oscillatory regime if delayed failure is assumed for different cases of slope stiffness and state of homogeneity/heterogeneity of the slope. At the end, we examine the influence of different frictional properties (along the sliding surface) on the conditions for the onset of instability. Results obtained indicate that the increase of friction parameters leads to stabilization of sliding for homogeneous geological environment. Moreover, increase of certain friction parameters leads to the occurrence of irregular aperiodic behavior, which could be ascribed to the regime of fast irregular sliding along the slope.
Understanding the Barriers and Enablers for Seeking Psychological Support Following a Burn Injury
Authors: McDermott Lianne , Hotton Matthew , Cartwright Anna
Published: July 2023

Burn injuries can be traumatic and distressing for patients, with a prolonged period of recovery. This qualitative study aimed to explore adult burn patients’ perceptions of the barriers and facilitators to accessing psychological support in a Regional Burns Service in Southeast England. Participants (five females and six males) were under the care of the burns unit and were not currently accessing psychological support. Eleven semi-structured interviews were conducted. Responses were analysed using thematic analysis. Four main themes highlighted how access to psychology was influenced by communication between the patient and service, beliefs about mental health, environmental challenges, and patient hope. Recommendations for improving access to burn psychological care included (1) the provision of patient resources to increase awareness and reduce stigma; (2) psychological skills training to encourage staff to recognise distress and respond appropriately; (3) staff training in the practice of cultural humility; (4) increasing psychological presence in outpatient appointments and via routine follow-ups.
Study to Live or Live to Study: The Link between Social Role Investment and Academic Success in First-Year Higher Education Students
Authors: Dias Diana , Santos Gina
Published: July 2023

Becoming a student, i.e., learning a set of new skills and lifestyles is an inevitable task for young people joining higher education (HE). Using Perrenoud’s (1995) conceptualization of the student’s role as a theoretical framework, this paper intends to reflect on the construction of students’ identities and its repercussions on their academic success through analysis of the discourse between HE students. How students try to intertwine their personal lives with the demands of their new roles as higher education students is also discussed. Qualitative data analysis was conducted using semi-structured, in-depth interviews with 30 engineering students. Our analysis of the results confirmed that attending HE can indeed be conceptualized as the exercise of a “craft”. This craft could be taught in different ways, with more or less success, in the light of the construction of one’s own social identity with more focus on either their role as student or their role as a young person. The results allow for the emergence of a conceptual framework which, crossing the investment in their social role as students with academic success, brings out distinctive dimensions: “Live to Study”, “Study to Live”, “Study without living” and “Live without study”. These dimensions provide four major student profiles that can advise the management of higher education institutions to strategically take actions to promote not only student success, but also the pedagogic efficiency of their educational programs.
Three-Dimensional Measurement of Full Profile of Steel Rail Cross-Section Based on Line-Structured Light
Authors: Liu Jiajia , Zhang Jiapeng , Ma Zhongli , Zhang Hangtian , Zhang Shun
Published: July 2023

The wear condition of steel rails directly affects the safety of railway operations. Line-structured-light visual measurement technology is used for online measurement of rail wear due to its ability to achieve high-precision dynamic measurements. However, in dynamic measurements, the random deviation of the measurement plane caused by the vibration of the railcar results in changes in the actual measured rail profile relative to its cross-sectional profile, ultimately leading to measurement deviations. To address these issues, this paper proposes a method for three-dimensional measurement of steel rail cross-sectional profiles based on binocular line-structured light. Firstly, calibrated dual cameras are used to simultaneously capture the profiles of both sides of the steel rail in the same world coordinate system, forming the complete rail profile. Then, considering that the wear at the rail waist is zero in actual operation, the coordinate of the circle center on both sides of the rail waist are connected to form feature vectors. The measured steel rail profile is aligned with the corresponding feature vectors of the standard steel rail model to achieve initial registration; next, the rail profile that has completed the preliminary matching is accurately matched with the target model based on the iterative closest point (ICP) algorithm. Finally, by comparing the projected complete rail profile onto the rail cross-sectional plane with the standard 3D rail model, the amount of wear on the railhead can be obtained. The experimental results indicate that the proposed line-structured-light measurement method for the complete rail profile, when compared to the measurements obtained from the rail wear gauge, exhibits smaller mean absolute deviation (MAD) and root mean square error (RMSE) for both the vertical and lateral dimensions. The MAD values for the vertical and lateral measurements are 0.009 mm and 0.039 mm, respectively, while the RMSE values are 0.011 mm and 0.048 mm. The MAD and RMSE values for the vertical and lateral wear measurements are lower than those obtained using the standard two-dimensional rail profile measurement method. Furthermore, it effectively eliminates the impact of vibrations during the dynamic measurement process, showcasing its practical engineering application value.
Blockchain for Healthcare Games Management
Authors: Chen Sheng , Cao Qi , Cai Yiyu
Published: July 2023

More and more serious games have been developed in recent years for patients with aging, rehabilitation, mental, and other healthcare needs. Often, such healthcare games have different experiments or evaluations associated. Rapid advancements in healthcare games see increased regulatory concerns. Unfortunately, there is no authority like the Food and Drug Administration (FDA) in United States for regulatory approvals of healthcare games. Yet, it is not appropriate to use the traditional pharmaceutical FDA approval, which is a tedious and time-consuming process, for healthcare games. We propose a Healthcare Game Blockchain (HGB) to support game developers, healthcare providers, healthcare authorities, and patients. For healthcare game developers, HGB is a common platform to deposit healthcare game prototypes or products. HGB can collect feedback data for analysis similar to drug clinical trials on efficacy and toxicity. Volunteers can be recruited for clinical trials using the healthcare games available on HGB. Once the healthcare games are accepted with a recommended usage, patient gamers can leverage the same HGB platform for treatments following the instructions of healthcare providers. HGB allows healthcare professionals to track the patient responses during their healthcare game medication. Big data analytics can be developed to monitor patient outcomes and healthcare game efficacy. Security and privacy issues are critical in healthcare data handling. HGB has the potential to resolve these limitations and inefficiencies improving data collection, data sharing, and data security by leveraging on the intrinsic properties that blockchain technology provides. This paper explores the possibility of integrating blockchain and healthcare games supporting clinical trials and treatment monitoring.
RETRACTED: Obodoekwe et al. Convolutional Neural Networks in Process Mining and Data Analytics for Prediction Accuracy. Electronics 2022, 11, 2128
Authors: Obodoekwe Ekene , Fang Xianwen , Lu Ke
Published: July 2023

The journal retracts the article entitled “Convolutional Neural Networks in Process Mining and Data Analytics for Prediction Accuracy” [...]
A Decoupled Semantic–Detail Learning Network for Remote Sensing Object Detection in Complex Backgrounds
Authors: Ruan Hao , Qian Wenbin , Zheng Zhihong , Peng Yingqiong
Published: July 2023

Detecting multi-scale objects in complex backgrounds is a crucial challenge in remote sensing. The main challenge is that the localization and identification of objects in complex backgrounds can be inaccurate. To address this issue, a decoupled semantic–detail learning network (DSDL-Net) was proposed. Our proposed approach comprises two components. Firstly, we introduce a multi-receptive field feature fusion and detail mining (MRF-DM) module, which learns higher semantic-level representations by fusing multi-scale receptive fields. Subsequently, it uses multi-scale pooling to preserve detail texture information at different scales. Secondly, we present an adaptive cross-level semantic–detail fusion (CSDF) network that leverages a feature pyramid with fusion between detailed features extracted from the backbone network and high-level semantic features obtained from the topmost layer of the pyramid. The fusion is accomplished through two rounds of parallel global–local contextual feature extraction, with shared learning for global context information between the two rounds. Furthermore, to effectively enhance fine-grained texture features conducive to object localization and features conducive to object semantic recognition, we adopt and improve two enhancement modules with attention mechanisms, making them simpler and more lightweight. Our experimental results demonstrate that our approach outperforms 12 benchmark models on three publicly available remote sensing datasets (DIOR, HRRSD, and RSOD) regarding average precision (AP) at small, medium, and large scales. On the DIOR dataset, our model achieved a 2.19% improvement in mAP@0.5 compared to the baseline model, with a parameter reduction of 14.07%.
Surrogate-Assisted Multi-Objective Optimisation of Transcritical Carbon Dioxide Scroll Expander Flank Clearance Based on Computational Fluid Dynamics
Authors: Du Yuheng , Li Shuang , Pekris Michael , Li Wei , Tian Guohong
Published: July 2023

Transcritical carbon dioxide waste heat recovery systems and the construction of scroll expanders have recently been hot topics. The flank clearance, located between the orbiting and fixed scroll, has a vital impact on the scroll expander performance. This paper estimates the effect of the flank clearance on the expander’s thermodynamic performance (first-law efficiency) based on computational fluid dynamics (CFD) simulations. The manufacturing cost of different flank clearances is also considered to enhance the feasibility of the machinery design. The computational cost for different flank clearance cases is significantly reduced with a surrogate-assisted multi-objective optimisation algorithm (SAMOA), which also supports modelling the trade-off relationship between manufacturing cost and machinery efficiency. The results indicated that an increasing flank clearance negatively affects the first-law thermal efficiency. The efficiency decreased from 87.41% to 44.83% moving from 20 to 200 μm flank clearances. The SAMOA successfully reduced the computational cost of the dynamic mesh CFD model from 90 h to 15 s with 0.6% discrepancy. The final Pareto solutions presented a clear trade-off relationship between the first-law efficiency and manufacturing cost and promised a diversity of optimum solutions. The “knee points” for the relationship were 25, 55, and 127 μm, which provided flexible clearance choices based on the importance of either machinery efficiency or manufacturing cost.
Potential Effects of Prebiotics on Gastrointestinal and Immunological Modulation in the Feeding of Healthy Dogs: A Review
Authors: Perini Mariana Pamplona , Pedrinelli Vivian , Marchi Pedro Henrique , Henríquez Lucas Ben Fiuza , Zafalon Rafael Vessecchi Amorim , Vendramini Thiago Henrique Annibale , Balieiro Julio César de Carvalho , Brunetto Marcio Antonio
Published: July 2023

One of the most studied functional foods in dog feed today is the prebiotic. Prebiotics are known for their modulating effects on the intestinal microbiota, fecal characteristics, and the immune system, which promotes beneficial effects to the host. However, with the diversity of prebiotics in the pet market, there are discussions around which prebiotics to use to stimulate these positive effects. In this case, the objective of this review was to demonstrate the main effects of different prebiotics on the feeding of healthy dogs. Platforms such as Embase, PubMed, and Mendeley were accessed to plot all scientific articles in vivo that reported prebiotics to feed adult or senior dogs. After excluding duplicate articles and without the evaluated criteria, we obtained a total of 36 articles. Our results demonstrated the diversity and concentrations of prebiotics in the feeding of healthy adult and senior dogs. The effects of prebiotics differ according to source, concentration, and length of the supplementation period. Several beneficial effects of different prebiotics have been observed in dogs, such as increased fecal Lactobacilli and Bifidobacteria concentrations and decreased fecal Clostridium perfringens and Escherichia coli concentrations, increased short chain fatty acids concentrations, decreased colonic ammonia absorption, and immunomodulatory effects, such as improved humoral immune response and increased phagocytic index. Galactooligosaccharides, fructooligosaccharides, mannanoligosaccharides, yeast cell wall, inulin, and beta-glucans were the most studied prebiotics, which showed potentially promising effects. This is a review that brings the importance and the modulating effects of prebiotics in the feeding of healthy dogs; the effects help the gastrointestinal tract and the immune system.
Is Chelidonichthys lucerna (Linnaeus, 1758) a Marine Estuarine-Dependent Fish? Insights from Saccular Otolith Microchemistry
Authors: Ferreira Inês , Daros Felippe A. , Moreira Cláudia , Feijó Diana , Rocha Alberto , Mendez-Vicente Ana , Pisonero Jorge , Correia Alberto Teodorico
Published: July 2023

Tub gurnard, Chelidonichthys lucerna (Linnaeus, 1758), is a Mediterranean-Atlantic benthic species usually captured as by-catch by Portuguese traditional fisheries and considered the most important commercial fish species of the family Triglidae. However, to date, little is known about its habitat residency and whether the species can be considered a marine estuarine-dependent fish. Otolith microchemistry has proven effective in providing information about fish movement patterns throughout different water salinities. In this study, core-to-edge transects of Sr:Ca in the fish saccular otoliths of 35 juveniles of C. lucerna captured in March 2007 by a scientific survey along the Portuguese coast were used to assess the species movement between brackish and marine waters. Data suggest that most individuals (97%) have an estuarine-dependent profile, with 63% showing a clear presence in marine waters during the early life history periods. Evidence of an estuarine residence throughout the fish life cycle was found in only 3% of individuals. Ba:Ca profiles did not reflect an inverse relationship with Sr:Ca salinity results but corroborated findings from other authors about the influence of upwelling processes and freshwater runoffs on Ba incorporation into the otoliths of coastal fish. Furthermore, the data also showed that C. lucerna can occupy and migrate among habitats with diverse salinity degrees, showing high environmental plasticity and adaptation.
Quantifying the Neural and Behavioral Correlates of Repeated Social Competition in the Fighting Fish Betta splendens
Authors: Dupeyron Solanch , Wallace Kelly J.
Published: July 2023

The fighting fish Betta splendens, long studied for its aggressive territorial competitions, has the potential to be a tractable and relevant model for studying the intersection of cognitive ecology and social neuroscience. Yet, few studies have comprehensively assessed Betta behavior across both social and nonsocial contexts. Furthermore, the present study is the first to quantify the expression of phosphorylated ribosomal protein S6 (PS6), a proxy for neural response, in the Betta telencephalon. Here, we assessed male Betta behavior across a suite of tasks and found that response to a mirror, but not neophilia (a novel object) nor anxiety (scototaxis), predicted behavior in a social competition. To then explore the cognitive aspects of social competition, we exposed Betta to either a familiar or novel opponent and compared their competitive behavior as well as their neural responses in the teleost homologs of the hippocampus, basolateral amygdala, and lateral septum. We did not detect any differences between familiar-exposed and novel-exposed individuals, but by implementing the first use of a habituation–dishabituation competition design in a study of Betta, we were able to observe remarkable consistency in competitive outcomes across repeated exposures. Taken together, the present study lays the groundwork for expanding the use of Betta to explore integrative and multidimensional questions of social cognition.
Techno-Functional and Sensory Characterization of Commercial Plant Protein Powders
Authors: Jakobson Kadi , Kaleda Aleksei , Adra Karl , Tammik Mari-Liis , Vaikma Helen , Kriščiunaite Tiina , Vilu Raivo
Published: July 2023

Many new plant proteins are appearing on the market, but their properties are insufficiently characterized. Hence, we collected 24 commercial proteins from pea, oat, fava bean, chickpea, mung bean, potato, canola, soy, and wheat, including different batches, and assessed their techno-functional and sensory properties. Many powders had yellow, red, and brown color tones, but that of fava bean was the lightest. The native pH ranged from 6.0 to 7.7. The water solubility index was 28% on average, but after heat treatment the solubility typically increased. Soy isolate had by far the best water-holding capacity of 6.3 g (H2O) g−1, and canola had the highest oil-holding capacity of 2.8 g (oil) g−1. The foaming capacity and stability results were highly varied but typical to the raw material. The emulsification properties of all powders were similar. Upon heating, the highest viscosity and storage modulus were found in potato, canola, and mung bean. All powders had raw material flavor, were bitter and astringent, and undissolved particles were perceived in the mouth. Large differences in functionality were found between the batches of one pea powder. In conclusion, we emphasize the need for methodological standardization, but while respecting the conditions found in end applications like meat and dairy analogs.
The Effect of Washing, Blanching and Frozen Storage on Pesticide Residue in Spinach
Authors: Flamminii Federica , Minetti Silvia , Mollica Adriano , Cichelli Angelo , Cerretani Lorenzo
Published: July 2023

Spinach (Spinacia oleracea L.) is a representative green leafy vegetable commonly consumed fresh or as a ready-to-cook frozen product, with increasing consumption because of its many health-related properties. Among leafy vegetables, spinach poses a major concern in terms of pesticide residue detection due to common phytotechnical practices. In this study, spinach leaves were treated in the open field with three commercial pesticide formulations containing propamocarb, lambda-cyhalothrin, fluopicolide and chlorantraniliprole at the highest concentration. The effects of the successive processing steps of washing, blanching, freezing and frozen storage were evaluated on the levels of the four pesticide residues and the degradation product (propamocarb n-desmethyl). The washing step caused a reduction of fluopicolide and chlorantraniliprole of −47% and −43%, respectively, while having a mild effect on lambda-cyhalothrin content (+5%). A two-minute blanching step allowed for the reduction of pesticides content ranging from −41% to −4% with respect to the washed sample. Different behaviors were depicted for longer blanching times, mainly for propamocarb, reaching −56% after 10 min of treatment. Processing factors higher than 1 were reported mainly for lambda-cyhalothrin and fluopicolide. Frozen storage led to a slight increase in the pesticide content in samples treated for 6 and 10 min. The optimal blanching treatment for spinach, submitted to freezing and frozen storage, seems to be 2 min at 80 °C.
Modification of Ginseng Insoluble Dietary Fiber by Enzymatic Method: Structural, Rheological, Thermal and Functional Properties
Authors: Jiang Guihun , Ramachandraiah Karna , Tan Chaoyi , Cai Nanjie , Ameer Kashif , Feng Xiaoyu
Published: July 2023

In this study, the effects of enzymatic modification using cellulase/xylanase on the composition and structural and functional properties of ginseng insoluble dietary fiber (G-IDF) were evaluated. Fourier transform infrared spectroscopy and scanning electron microcopy showed that enzymatic extraction treatment caused obvious structural alterations in ginseng-modified (G-MIDF) samples, which exhibited more porous and completely wrinkled surfaces. Comparing the peak morphology of G-MIDF with untreated IDF using X-ray diffractometry, the G-MIDF sample exhibited split peaks at a 2θ angle of 23.71°, along with the emergence of sharp peaks at 28.02°, 31.78°, and 35.07°. Thermo-gravimetric analysis showed that G-MIDF exhibited a specified range of pyrolysis temperature and is suitable for food applications involving processing at temperatures below 300 °C. Overall, it was evident from rheograms that both G-IDF and G-MIDF exhibited a resemblance with respect to viscosity changes as a function of the shear rate. Enzymatic treatment led to significant (p < 0.05) improvement in water holding, oil retention, water swelling, nitrite ion binding, bile acid binding, cholesterol absorption, and glucose absorption capacities.
Mechanism of Anti-Diabetic Activity from Sweet Potato (Ipomoea batatas): A Systematic Review
Authors: Arisanti Cokorda Istri Sri , Wirasuta I. Made Agus Gelgel , Musfiroh Ida , Ikram Emmy Hainida Khairul , Muchtaridi Muchtaridi
Published: July 2023

This study aims to provide an overview of the compounds found in sweet potato (Ipomoea batatas) that contribute to its anti-diabetic activity and the mechanisms by which they act. A comprehensive literature search was conducted using electronic databases, such as PubMed, Scopus, and Science Direct, with specific search terms and Boolean operators. A total of 269 articles were initially retrieved, but after applying inclusion and exclusion criteria only 28 articles were selected for further review. Among the findings, four varieties of sweet potato were identified as having potential anti-diabetic properties. Phenolic acids, flavonols, flavanones, and anthocyanidins are responsible for the anti-diabetic activity of sweet potatoes. The anti-diabetic mechanism of sweet potatoes was determined using a combination of components with multi-target actions. The results of these studies provide evidence that Ipomoea batatas is effective in the treatment of type 2 diabetes.
Comparative Quantitation of Kokumi γ-Glutamyl Peptides in Spanish Dry-Cured Ham under Salt-Reduced Production
Authors: Heres Alejandro , Li Qian , Toldrá Fidel , Lametsch René , Mora Leticia
Published: July 2023

Salting is a crucial step during the production of dry-cured ham and it is not well known whether it has an impact on the generation of taste-active peptides. The present study focused on the quantitation of kokumi γ-glutamyl peptides in low-salted Spanish dry-cured hams with 12 months of processing. By using mass spectrometry, peptides were quantitated from samples obtained after ethanolic deproteinization-based and non-ethanolic deproteinization-based extraction methods. Peptides γ-EA, γ-EE, and γ-EL registered mean values of 0.31, 2.75, and 11.35 µg/g of dry-cured ham, respectively, with no differences observed between both extraction protocols. However, γ-EF, γ-EM, γ-EV, γ-EW, γ-EY, and γ-EVG presented significantly (p < 0.05) higher concentrations in the ethanolic deproteinized samples showing values of 5.58, 4.13, 13.90, 0.77, 3.71, and 0.11 µg/g of dry-cured ham, respectively. These outcomes reflect the importance of protocols for the extraction of peptides to achieve the most feasible results. In addition, potential precursors for the formation of γ-glutamyl peptides are generated during dry-curing under salt restriction. The kokumi activity of these γ-glutamyl peptides could enhance the sensory attributes countering the taste deficiencies caused by the salt restriction.
Creep Properties of a Viscoelastic 3D Printed Sierpinski Carpet-Based Fractal
Authors: Pascual-Francisco Juan B. , Susarrey-Huerta Orlando , Farfan-Cabrera Leonardo I. , Flores-Hernández Rockali
Published: July 2023

In this paper, the phenomenon of creep compliance and the creep Poisson’s ratio of a 3D-printed Sierpinski carpet-based fractal and its bulk material (flexible resin Resione F69) was experimentally investigated, as well as the quantification of the change in the viscoelastic parameters of the material due to the fractal structure. The samples were manufactured via a vat photopolymerization method. The fractal structure of the samples was based on the Sierpinski carpet at the fourth iteration. In order to evaluate the response of both the fractal and the bulk material under the creep phenomenon, 1 h-duration tensile creep tests at three constant temperatures (20, 30 and 40 °C) and three constant stresses (0.1, 0.2 and 0.3 MPa) were conducted. A digital image correlation (DIC) technique was implemented for strain measurement in axial and transverse directions. From the results obtained, the linear viscoelastic behavior regime of the fractal and the bulk material was identified. The linear viscoelastic parameters of both fractal and bulk materials were then estimated by fitting the creep Burgers model to the experimental data to determine the effect of the fractal geometry on the viscoelastic properties of the samples. Overall, it was found that the reduction in stiffness induced by the fractal porosity caused a more viscous behavior of the material and a reduction in its creep Poisson’s ratio, which means an increase in the compliance of the material.
Chitosan-Based Hydrogel in the Management of Dermal Infections: A Review
Authors: Mohite Popat , Rahayu Pudji , Munde Shubham , Ade Nitin , Chidrawar Vijay R. , Singh Sudarshan , Jayeoye Titilope J. , Prajapati Bhupendra G. , Bhattacharya Sankha , Patel Ravish J.
Published: July 2023

The main objective of this review is to provide a comprehensive overview of the current evidence regarding the use of chitosan-based hydrogels to manage skin infections. Chitosan, a naturally occurring polysaccharide derived from chitin, possesses inherent antimicrobial properties, making it a promising candidate for treating various dermal infections. This review follows a systematic approach to analyze relevant studies that have investigated the effectiveness of chitosan-based hydrogels in the context of dermal infections. By examining the available evidence, this review aims to evaluate these hydrogels’ overall efficacy, safety, and potential applications for managing dermal infections. This review’s primary focus is to gather and analyze data from different recent studies about chitosan-based hydrogels combating dermal infections; this includes assessing their ability to inhibit the growth of microorganisms and reduce infection-related symptoms. Furthermore, this review also considers the safety profile of chitosan-based hydrogels, examining any potential adverse effects associated with their use. This evaluation is crucial to ensure that these hydrogels can be safely utilized in the management of dermal infections without causing harm to patients. The review aims to provide healthcare professionals and researchers with a comprehensive understanding of the current evidence regarding the use of chitosan-based hydrogels for dermal infection management. The findings from this review can contribute to informed decision-making and the development of potential treatment strategies in this field.
3D Co-Printing and Substrate Geometry Influence the Differentiation of C2C12 Skeletal Myoblasts
Authors: Loi Giada , Scocozza Franca , Aliberti Flaminia , Rinvenuto Lorenza , Cidonio Gianluca , Marchesi Nicola , Benedetti Laura , Ceccarelli Gabriele , Conti Michele
Published: July 2023

Cells are influenced by several biomechanical aspects of their microenvironment, such as substrate geometry. According to the literature, substrate geometry influences the behavior of muscle cells; in particular, the curvature feature improves cell proliferation. However, the effect of substrate geometry on the myogenic differentiation process is not clear and needs to be further investigated. Here, we show that the 3D co-printing technique allows the realization of substrates. To test the influence of the co-printing technique on cellular behavior, we realized linear polycaprolactone substrates with channels in which a fibrinogen-based hydrogel loaded with C2C12 cells was deposited. Cell viability and differentiation were investigated up to 21 days in culture. The results suggest that this technology significantly improves the differentiation at 14 days. Therefore, we investigate the substrate geometry influence by comparing three different co-printed geometries—linear, circular, and hybrid structures (linear and circular features combined). Based on our results, all structures exhibit optimal cell viability (>94%), but the linear pattern allows to increase the in vitro cell differentiation, in particular after 14 days of culture. This study proposes an endorsed approach for creating artificial muscles for future skeletal muscle tissue engineering applications.
Structural Characteristics of Mitochondrial Genomes of Eight Treehoppers (Hemiptera: Membracidae: Centrotinae) and Their Phylogenetic Implications
Authors: Bai Haijun , Zhang Jinrui , Dietrich Christopher H. , Li Yiping , Yuan Xiangqun
Published: July 2023

Complete mitochondrial genomes were newly sequenced for eight species of the treehopper subfamily Centrotinae (Hemiptera: Membracidae), four of which represent genera for which mitogenomes were not previously available. The new mitogenomes are generally similar in overall structure, gene order, base composition, and nucleotide content to those of previously sequenced species of the subfamily. Phylogenetic analyses were conducted using both maximum likelihood and Bayesian inference methods based on three separate nucleotide sequence datasets in which RNA gene sequences and/or third codon positions were either included or excluded from the concatenated protein-coding gene alignments. The results are consistent with previous phylogenies based on morphology and partial nuclear genome data, except for the lack of support for the monophyly of Leptocentrini. These results show that mitogenome sequences are informative of both ancient and recent divergence patterns within Centrotinae.
Implications in Halotherapy of Aerosols from the Salt Mine Targu Ocna—Structural-Functional Characteristics
Authors: Antonovici (Munteanu) Mihaela Orlanda , Sandu Ioan Gabriel , Vasilache Viorica , Sandu Andrei Victor , Arcana Stefanita , Arcana Raluca Ioana , Sandu Ion
Published: July 2023

The paper presents the evolution of the concentration level for four particle size groups of microaerosols (1.0, 2.5, 4.0 and 10.0 µm) in correlation with the microclimatic characteristics (temperature, humidity, lighting, pressure and concentration in CO2 and O2) in three active areas of the Targu Ocna Saltworks, currently used in treatments with solions (hydrated aerosols): in the vicinity of the walls of the old mining salt room, where there is a semi-wet static regime (SSR); in the transition area between the old rooms of exploitation with the semi-wet dynamic regime (DSR); and in the area of the waterfall and the marshy lake with the dynamic wet regime (DWR). The first and last halochamber are the ones recommended for cardio–respiratory, immuno–thyroid and osteo–muscular conditions, as well as in psycho–motor disorders. Based on questionnaires carried out over the course of a year, between 1 September 2021–31 August 2022, in two periods of stationing/treatment: a cold one (15 September 2021–15 December 2021) and a warm one (1 May 2022–30 July 2022), correlated with the data from the Salina medical office, achieved the profile of the improvement rate of the patients’ ailments depending on the type of treatment (working regime in halochambers). These studies have allowed the optimization of the treatment conditions in the artificial surface halochambers in order to reduce the stationary period and optimize the treatment cycles.