bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhancing Supplemental Effects of Acute Natural Antioxidant Derived from Yeast Fermentation and Vitamin C on Sports Performance in Triathlon Athletes: A Randomized, Double-Blinded, Placebo-Controlled, Crossover Trial
Authors: Lee Eunjoo , Park Hun-Young , Kim Sung-Woo , Sun Yerin , Choi Jae-Ho , Seo Jisoo , Jung Yanghoon Peter , Kim Ah-Jin , Kim Jisu , Lim Kiwon
Published: July 2023

This study investigated the acute effects of natural antioxidants, derived from yeast fermentation containing glutathione and dietary vitamin C supplementation, on metabolic function, skeletal muscle oxygenation, cardiac function, and antioxidant function during submaximal exercise in middle-aged triathlon athletes. Twelve participants (aged 49.42 ± 5.9 years) completed 90 min submaximal cycling trials corresponding to 70% maximal oxygen uptake with either vitamin C and glutathione (VitC+Glu), vitamin C (VitC), glutathione (Glu) supplementation, or placebo. Metabolic function (minute ventilation, oxygen uptake, carbon dioxide output [VCO2], respiratory exchange ratio [RER], oxygen pulse [O2pulse], carbohydrate oxidation, fat oxidation, and energy expenditure), skeletal muscle oxygenation (oxidized hemoglobin and myoglobin in skeletal muscle tissue, total hemoglobin and myoglobin in skeletal muscle tissue [tHb]), cardiac function (heart rate [HR], stroke volume [SV], cardiac output, end-diastolic volume, end-systolic volume, and ejection fraction), and antioxidant function parameters (blood lactate, superoxide dismutase, catalase, glutathione peroxidases, glutathione [GSH], diacron reactive oxygen metabolite [dROM], and biological antioxidant potential [BAP]) were measured during submaximal exercise and recovery. VCO2, RER, HR, blood lactate after exercise, and dROM were significantly lower, and O2pulse, tHb, and BAP were significantly higher for VitC+Glu than for the other trials (p < 0.05). In conclusion, combined vitamin C and glutathione supplementation was more effective in improving metabolic function, skeletal oxygenation, cardiac function, and antioxidant function during prolonged submaximal exercise in middle-aged triathletes.
Exploring the Effects of a Mediterranean Diet and Weight Loss on the Gut Microbiome and Cognitive Performance in Older, African American Obese Adults: A Post Hoc Analysis
Authors: McLeod Andrew , Bernabe Beatriz Penalver , Xia Yinglin , Sanchez-Flack Jennifer , Lamar Melissa , Schiffer Linda , Hemphill Nefertiti Oji-Njideka , Fantuzzi Giamila , Maki Pauline , Fitzgibbon Marian , Tussing-Humphreys Lisa
Published: July 2023

African American adults have a higher prevalence of Alzheimer’s dementia (AD) than non-Hispanic Whites. The impact of a Mediterranean Diet (Med Diet) and intentional weight loss (IWL) on the gut microbiome may alter AD risk. A post hoc analysis of the Building Research in Diet and Cognition (BRIDGE) trial was performed to determine whether participation in an 8-month Med Diet lifestyle intervention with (n = 35) or without IWL (n = 31) was associated with changes in gut microbiota structure, abundance, and function and whether these changes were related to changes in cognitive performance. The results showed that family and genus alpha diversity increased significantly in both groups combined (p = 0.0075 and p = 0.024, respectively). However, there were no other significant microbially related within- or between-group changes over time. Also, an increase in Med Diet adherence was significantly associated with a decrease in alpha diversity at the phylum level only (p = 0.049). Increasing alpha diversity was associated with decreasing cognitive performance, but this association was attenuated after controlling for Med Diet adherence. In sum, an 8-month Med Diet lifestyle intervention with or without IWL did not appreciably alter the gut microbiome.
The Role of Next-Generation Sequencing (NGS) in the Management of Tuberculosis: Practical Review for Implementation in Routine
Authors: Beviere Marion , Reissier Sophie , Penven Malo , Dejoies Loren , Guerin François , Cattoir Vincent , Piau Caroline
Published: July 2023

Next-generation sequencing (NGS) has modernized the field of tuberculosis (TB) research by enabling high-throughput sequencing of the entire genome of Mycobacterium tuberculosis (MTB), which is the causative agent of TB. NGS has provided insights into the genetic diversity of MTB, which are crucial for understanding the evolution and transmission of the disease, and it has facilitated the identification of drug-resistant strains, enabling rapid and accurate tailoring of treatment. However, the high cost and the technical complexities of NGS currently limit its widespread use in clinical settings. International recommendations are thus necessary to facilitate the interpretation of polymorphisms, and an experimental approach is still necessary to correlate them to phenotypic data. This review aims to present a comparative, step-by-step, and up-to-date review of the techniques available for the implementation of this approach in routine laboratory workflow. Ongoing research on NGS for TB holds promise for improving our understanding of the disease and for developing more efficacious treatments.
Corynebacterium ulcerans Infections in Eurasian Beavers (Castor fiber)
Authors: Sting Reinhard , Pölzelbauer Catharina , Eisenberg Tobias , Bonke Rebecca , Blazey Birgit , Peters Martin , Riße Karin , Sing Andreas , Berger Anja , Dangel Alexandra , Rau Jörg
Published: July 2023

The Eurasian beaver (Castor fiber) has been reintroduced successfully in Germany since the 1990s. Since wildlife is an important source of zoonotic infectious diseases, monitoring of invasive and reintroduced species is crucial with respect to the One Health approach. Three Eurasian beavers were found dead in the German federal states of Bavaria, North Rhine–Westphalia and Baden–Wuerttemberg in 2015, 2021 and 2022, respectively. During post-mortem examinations, Corynebacterium (C.) ulcerans could be isolated from the abscesses of two beavers and from the lungs of one of the animals. Identification of the bacterial isolates at the species level was carried out by spectroscopic analysis using MALDI-TOF MS, FT-IR and biochemical profiles and were verified by molecular analysis based on 16-23S internal transcribed spacer (ITS) region sequencing. Molecular characterization of the C. ulcerans isolates using whole-genome sequencing (WGS) revealed a genome size of about 2.5 Mbp and a GC content of 53.4%. Multilocus sequence typing (MLST) analysis classified all three isolates as the sequence type ST-332. A minimum spanning tree (MST) based on cgMLST allelic profiles, including 1211 core genes of the sequenced C. ulcerans isolates, showed that the beaver-derived isolates clearly group on the branch of C. ulcerans with the closest relationship to each other, in close similarity to an isolate from a dog. Antibiotic susceptibility testing revealed resistance to clindamycin and, in one strain, to erythromycin according to EUCAST, while all isolates were susceptible to the other antimicrobials tested.
Dogs Harbor Leishmania braziliensis and Participate in the Transmission Cycle of Human Tegumentary Leishmaniasis
Authors: Lago Jamile , Fraga Deborah , Coelho Lívia , Jesus Matheus Silva de , Leite Bruna , Werneck Guilherme L. , Arruda Sérgio , Lago Ednaldo , Carvalho Edgar M. , Bacellar Olivia
Published: July 2023

Dogs play an important role in transmission of Leishmania infantum, but epidemiologic and clinical studies of canine tegumentary leishmaniasis (CTL) are scarce. In an endemic area of human American tegumentary leishmaniasis (ATL) caused by Leishmania braziliensis, we determine the prevalence and incidence of both CTL and subclinical (SC) L. braziliensis infection in dogs and evaluated if the presence of dogs with CTL or SC L. braziliensis infection is associated with the occurrence of human ATL. SC infection in healthy animals and CTL in animals with ulcers were determined by PCR on biopsied healthy skin or on ulcers or by detecting antibodies against soluble leishmania antigen. We compared the occurrence of human ATL in homes with dogs with CTL or SC infection with control homes without dogs or with dogs without CTL or SC infection. The prevalence of SC infection was 35% and of CTL 31%. The incidence of SC infection in dogs was 4.6% and of CTL 9.3%. The frequency of ATL in humans was 50% in homes with infected dogs and 13% in homes without L. braziliensis infection in dogs. CTL and SC infection is highly prevalent, and dogs may participate in the transmission chain of L. braziliensis.
Engineering of Nisin as a Means for Improvement of Its Pharmacological Properties: A Review
Authors: Musiejuk Mateusz , Kafarski Paweł
Published: July 2023

Lantibiotics are believed to have a conceivable potential to be used as therapeutics, especially against clinically resistant bacterial strains. However, their low solubility and poor stability under physiological conditions limit their availability for clinical studies and further pharmaceutical commercialization. Nisin is a readily available and cheap lanthipeptide and thus serves as a good model in the search for the tools to engineer lantibiotics with improved pharmacological properties. This review aims to address technologies that can be applied to alter and enhance the antimicrobial activity, antibacterial spectrum and physicochemical properties (solubility, solution stability and protease resistance) of nisin. There are basically two general means to obtain nisin analogs—protein engineering and chemical functionalization of this antibiotic. Although bioengineering techniques have been well developed and enable the creation of nisin mutants of variable structures and properties, they are lacking spectacular effects so far. Chemical modifications of nisin based on utilization of the reactivity of its free amino and carboxylic moieties, as well as reactivity of the double bonds of its dehydroamino acids, are in their infancy.
Induced Vascular Normalization—Can One Force Tumors to Surrender to a Better Microenvironment?
Authors: Sun Xu Xin , Nosrati Zeynab , Ko Janell , Lee Che-Min , Bennewith Kevin L. , Bally Marcel B.
Published: July 2023

Immunotherapy has changed the way many cancers are being treated. Researchers in the field of immunotherapy and tumor immunology are investigating similar questions: How can the positive benefits achieved with immunotherapies be enhanced? Can this be achieved through combinations with other agents and if so, which ones? In our view, there is an urgent need to improve immunotherapy to make further gains in the overall survival for those patients that should benefit from immunotherapy. While numerous different approaches are being considered, our team believes that drug delivery methods along with appropriately selected small-molecule drugs and drug candidates could help reach the goal of doubling the overall survival rate that is seen in some patients that are given immunotherapeutics. This review article is prepared to address how immunotherapies should be combined with a second treatment using an approach that could realize therapeutic gains 10 years from now. For context, an overview of immunotherapy and cancer angiogenesis is provided. The major targets in angiogenesis that have modulatory effects on the tumor microenvironment and immune cells are highlighted. A combination approach that, for us, has the greatest potential for success involves treatments that will normalize the tumor’s blood vessel structure and alter the immune microenvironment to support the action of immunotherapeutics. So, this is reviewed as well. Our focus is to provide an insight into some strategies that will engender vascular normalization that may be better than previously described approaches. The potential for drug delivery systems to promote tumor blood vessel normalization is considered.
A Novel PWM Signal-Generation Strategy for Pockels Cell Drivers
Authors: Wu Yajun , Liu Wenqing , Chen Jinxin , Sun Xinhui , Zhang Tianshu
Published: July 2023

A Pockels cell driver (PCD) can be viewed as a high-voltage pulse width generator for controlling the bi-refringence effect of electro-optical crystals. The main features of a PCD include a high repetition rate, fast on and off switching, variable pulse duration, and a true square pulse shape. The most commonly used PCD has a narrow pulse width tuning range, typically within a few microseconds. In this paper, we propose a PCD based on a novel pulse width modulation (PWM) signal-generation strategy that can continuously adjust its pulse width with a minimum step size of 10 ns and no restriction on the maximum width. Therefore, it is easily compatible with both “On-type” and “Off-type” applications of the electro-optic crystal quarter-wave voltage. The experimental results show that the rising and falling times of the proposed PCD are approximately 7.3 ns and 7.8 ns, respectively, with a maximum repetition rate of 1 MHz and a maximum voltage of approximately 2.0 kV. Finally, the functionality of the PCD is demonstrated in a home-built slab laser.
High-Directionality Silicon Nitride Antenna Based on Distributed Bragg Reflector for Optical Phased Array
Authors: Wei Jinmei , Li Yan , Zhang Yuejun , Dai Tingge , Li Xiangyu , Zhang Xiaowei , Wang Ying
Published: July 2023

Optical phased arrays (OPAs) have great potential in the fields of integrated solid-state light detection and ranging. The ranging distance of an OPA can be further enlarged by improving the directionality of the grating antenna. A high-directionality silicon nitride grating antenna with a distributed Bragg reflector (DBR) is proposed. The DBR consists of a stack of silicon nitride and silicon dioxide layers, which are utilized as the bottom reflectors to further reduce downward radiation. In a simulation, the directionality of the antenna exceeded 71.6% within the wavelength range of 1420–1740 nm. Additionally, the directionality of the antenna can achieve 97.6% at 1550 nm. Compared to a grating antenna without a DBR, the directionality is improved by 1.52 dB. Moreover, the proposed silicon nitride grating antenna has a large fabrication tolerance and is compatible with CMOS fabrication techniques, showing great potential for enhancing the performance of the integrated optical phased array.
High-Quality Genome Assembly and Genome-Wide Association Study of Male Sterility Provide Resources for Flax Improvement
Authors: Zhao Xiaoqing , Yi Liuxi , Zuo Yongchun , Gao Fengyun , Cheng Yuchen , Zhang Hui , Zhou Yu , Jia Xiaoyun , Su Shaofeng , Zhang Dejian , Zhang Xiangqian , Ren Yongfeng , Mu Yanxin , Jin Xiaolei , Li Qiang , Bateer Siqin , Lu Zhanyuan
Published: July 2023

Flax is an economic crop with a long history. It is grown worldwide and is mainly used for edible oil, industry, and textiles. Here, we reported a high-quality genome assembly for “Neiya No. 9”, a popular variety widely grown in China. Combining PacBio long reads, Hi-C sequencing, and a genetic map reported previously, a genome assembly of 473.55 Mb was constructed, which covers ~94.7% of the flax genome. These sequences were anchored onto 15 chromosomes. The N50 lengths of the contig and scaffold were 0.91 Mb and 31.72 Mb, respectively. A total of 32,786 protein-coding genes were annotated, and 95.9% of complete BUSCOs were found. Through morphological and cytological observation, the male sterility of flax was considered dominant nuclear sterility. Through GWAS analysis, the gene LUSG00017705 (cysteine synthase gene) was found to be closest to the most significant SNP, and the expression level of this gene was significantly lower in male sterile plants than in fertile plants. Among the significant SNPs identified in the GWAS analysis, only two were located in the coding region, and these two SNPs caused changes in the protein encoded by LUSG00017565 (cysteine protease gene). It was speculated that these two genes may be related to male sterility in flax. This is the first time the molecular mechanism of male sterility in flax has been reported. The high-quality genome assembly and the male sterility genes revealed, provided a solid foundation for flax breeding.
Genome-Wide Analysis of WRKY and NAC Transcription Factors in Carica papaya L. and Their Possible Role in the Loss of Drought Tolerance by Recent Cultivars through the Domestication of Their Wild Ancestors
Authors: Arroyo-Álvarez Erick , Chan-León Arianna , Girón-Ramírez Amaranta , Fuentes Gabriela , Estrella-Maldonado Humberto , Santamaría Jorge M.
Published: July 2023

A genome-wide analysis for two families of key transcription factors (TF; WRKY and NAC) involved in drought response revealed 46 WRKY and 66 NAC members of the Carica papaya genome. A phylogenetic analysis grouped the CpWRKY proteins into three groups (I, II a, b, c, d, e and III), while the CpNAC proteins were clustered into 15 groups. The conserved domains, chromosomal localization and promoter cis-acting elements were also analyzed. In addition, from a previous transcriptome study of two contrasting genotypes in response to 14 days of water deficit stress (WDS), we found that 29 of the 46 CpWRKYs genes and 25 of the 66 CpNACs genes were differentially expressed in response to the WDS. In the present paper, the native wild genotype (WG) (collected in its center of origin) consistently showed a higher expression (transcripts per million; TPM and fold change; FC) than the commercial genotype (CG) in almost all the members of the CpWRKY and CpNAC gene families. To corroborate this, we selected CpWRKY50 and CpNAC83.1 for further evaluation by RT-qPCR. Consistently, the WG showed higher relative expression levels (REL) after 14 days of WDS than the CG, in both the leaves and roots. The results suggest that the CpWRKY and CpNAC TF families are important for drought tolerance in this species. The results may also suggest that, during the domestication process, the ability of the native (wild) C. papaya genotypes to respond to drought (including the overexpression of the CpWRKY and CpNAC genes) was somehow reduced in the current commercial genotypes.
Modeling of the Spatial Distribution of Forest Carbon Storage in a Tropical/Subtropical Island with Multiple Ecozones
Authors: Chang Ting-Wei , Chen Guan-Fu , Chang Ken-Hui
Published: July 2023

Visual data on the geographic distribution of carbon storage help policy makers to formulate countermeasures for global warming. However, Taiwan, as an island showing diversity in climate and topography, had lacked valid visual data on the distribution of forest carbon storage between the last two forest surveys (1993–2015). This study established a model to estimate and illustrate the distribution of forest carbon storage. This model uses land use, stand morphology, and carbon conversion coefficient databases accordingly for 51 types of major forests in Taiwan. An estimation in 2006 was conducted and shows an overall carbon storage of 165.65 Mt C, with forest carbon storage per unit area of 71.56 t C ha−1, where natural forests and plantations respectively contributed 114.15 Mt C (68.9%) and 51.50 Mt C (31.1%). By assuming no change in land use type, the carbon sequestration from 2006 to 2007 by the 51 forest types was estimated to be 5.21 Mt C yr−1 using historical tree growth and mortality rates. The result reflects the reality of the land use status and the events of coverage shifting with time by combining the two forest surveys in Taiwan.
Structure-Guided Identification of Critical Residues in the Vacuolar Cation/Proton Antiporter NHX1 from Arabidopsis thaliana
Authors: Rombolá-Caldentey Belén , Mendoza Imelda , Quintero Francisco J. , Pardo José M.
Published: July 2023

Cation/Proton Antiporters (CPA) acting in all biological membranes regulate the volume and pH of cells and of intracellular organelles. A key issue with these proteins is their structure–function relationships since they present intrinsic regulatory features that rely on structural determinants, including pH sensitivity and the stoichiometry of ion exchange. Crystal structures are only available for prokaryotic CPA, whereas the eukaryotic ones have been modeled using the former as templates. Here, we present an updated and improved structural model of the tonoplast-localized K+,Na+/H+ antiporter NHX1 of Arabidopsis as a representative of the vacuolar NHX family that is essential for the accumulation of K+ into plant vacuoles. Conserved residues that were judged as functionally important were mutated, and the resulting protein variants were tested for activity in the yeast Saccharomyces cerevisiae. The results indicate that residue N184 in the ND-motif characteristic of CPA1 could be replaced by the DD-motif of CPA2 family members with minimal consequences for their activity. Attempts to alter the electroneutrality of AtNHX1 by different combinations of amino acid replacements at N184, R353 and R390 residues resulted in inactive or partly active proteins with a differential ability to control the vacuolar pH of the yeast.
Effects of Different Heating Treatments on the Antioxidant Activity and Phenolic Compounds of Ecuadorian Red Dacca Banana
Authors: Tuárez-García Diego Armando , Galván-Gámez Hugo , Erazo Solórzano Cyntia Yadira , Edison Zambrano Carlos , Rodríguez-Solana Raquel , Pereira-Caro Gema , Sánchez-Parra Mónica , Moreno-Rojas José M. , Ordóñez-Díaz José L.
Published: July 2023

The banana is a tropical fruit characterized by its composition of healthy and nutritional compounds. This fruit is part of traditional Ecuadorian gastronomy, being consumed in a wide variety of ways. In this context, unripe Red Dacca banana samples and those submitted to different traditional Ecuadorian heating treatments (boiling, roasting, and baking) were evaluated to profile their phenolic content by ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) and the antioxidant activity by ORAC, ABTS, and DPPH assays. A total of sixty-eight phenolic compounds were identified or tentatively identified in raw banana and treated samples, highlighting the content in flavonoids (flavan-3-ols with 88.33% and flavonols with 3.24%) followed by the hydroxybenzoic acid family (5.44%) in raw banana samples. The total phenolic compound content significantly decreased for all the elaborations evaluated, specifically from 442.12 mg/100 g DW in fresh bananas to 338.60 mg/100 g DW in boiled (23.41%), 243.63 mg/100 g DW in roasted (44.90%), and 109.85 mg/100 g DW in baked samples (75.15%). Flavan-3-ols and flavonols were the phenolic groups most affected by the heating treatments, while flavanones and hydroxybenzoic acids showed higher stability against the heating treatments, especially the boiled and roasted samples. In general, the decrease in phenolic compounds corresponded with a decline in antioxidant activity, evaluated by different methods, especially in baked samples. The results obtained from PCA studies confirmed that the impact of heating on the composition of some phenolic compounds was different depending on the technique used. In general, the heating processes applied to the banana samples induced phytochemical modifications. Even so, they remain an important source of bioactive compounds for consumers.
On the Scarce Occurrence of Arsenic in Vineyard Soils of Castilla La Mancha: Between the Null Tolerance of Vine Plants and Clean Vineyards
Authors: Jiménez-Ballesta Raimundo , García-Navarro Francisco J. , Amorós José A. , Pérez-de-los-Reyes Caridad , Bravo Sandra
Published: July 2023

Arsenic (As), a widely distributed metalloid in the Earth’s crust, constitutes one of the most significant environmental contaminants today. This study was carried out to determine As concentrations in the soils of a Castilla La Mancha (CLM; Spain) benchmark collection that represents all the soil orders (soil taxonomy) in this territory. It also examined vine plant tolerance to As in relation to soil concentration. For this purpose, soils and leaves from vineyards were collected from 10 locations in the CLM community. The bioconcentration factor (BCF) of As in vineyards was assessed. The results of the present study show that As content in soils is widely variable, and is fundamentally related to soil type and parent material. The most surprising point is that, although some vineyards have been treated with As derivatives, the vast majority of them do not accumulate any amount of As. This important finding must be used to enhance the quality of the final obtained product: wine. In other words, CLM wines are not at risk of As contamination and must, therefore, be clean wines. Our results suggest that CLM vineyards are clean of contamination by As because this element in leaves reveals null vine capacity to accumulate As, a process that derives from scarce As in soils and the traditional practices carried out by winegrowers.
Long-Chain Branched Bio-Based Poly(butylene dodecanedioate) Copolyester Using Pentaerythritol as Branching Agent: Synthesis, Thermo-Mechanical, and Rheological Properties
Authors: Niu Ruixue , Zheng Zhening , Lv Xuedong , He Benqiao , Chen Sheng , Zhang Jiaying , Ji Yanhong , Liu Yi , Zheng Liuchun
Published: July 2023

The introduction of long-chain branched structures into biodegradable polyesters can effectively improve the melt strength and blow-molding properties of polyesters. In this study, pentaerythritol (PER) was used as a branching agent to synthesize branched poly(butylene dodecanedioate) (PBD), and the resulting polymers were characterized by Nuclear Magnetic Resonance Proton Spectra (1H NMR) and Fourier Transform Infrared spectroscopy (FT-IR). It was found that the introduction of a small amount of PER (0.25–0.5 mol%) can generate branching and even crosslinking structures. Both impact strength and tensile modulus can be greatly improved by the introduction of a branching agent. With the introduction of 1 mol% PER content in PBD, the notched impact strength of PBD has been increased by 85%, and the tensile modulus has been increased by 206%. Wide-angle X-ray diffraction and differential scanning calorimetry results showed that PER-branched PBDs exhibited improved crystallization ability compared with linear PBDs. Dynamic viscoelastics revealed that shear-thickening behaviors can be found for all branched PBD under low shear rates.
Nanocomposite Hydrogel Films Based on Sequential Interpenetrating Polymeric Networks as Drug Delivery Platforms
Authors: Toader Gabriela , Podaru Alice Ionela , Diacon Aurel , Rusen Edina , Mocanu Alexandra , Brincoveanu Oana , Alexandru Mioara , Zorila Florina Lucica , Bacalum Mihaela , Albota Florin , Gavrila Ana Mihaela , Trica Bogdan , Rotariu Traian , Ionita Mariana , Istrate Marcel
Published: July 2023

In this study, novel materials have been obtained via a dual covalent and ionic crosslinking strategies, leading to the formation of a fully interpenetrated polymeric network with remarkable mechanical performances as drug delivery platforms for dermal patches. The polymeric network was obtained by the free-radical photopolymerization of N-vinylpyrrolidone using tri(ethylene glycol) divinyl ether as crosslinker in the presence of sodium alginate (1%, weight%). The ionic crosslinking was achieved by the addition of Zn2+, ions which were coordinated by the alginate chains. Bentonite nanoclay was incorporated in hydrogel formulations to capitalize on its mechanical reinforcement and adsorptive capacity. TiO2 and ZnO nanoparticles were also included in two of the samples to evaluate their influence on the morphology, mechanical properties and/or the antimicrobial activity of the hydrogels. The double-crosslinked nanocomposite hydrogels presented a good tensile resistance (1.5 MPa at 70% strain) and compression resistance (12.5 MPa at a strain of 70%). Nafcillin was loaded into nanocomposite hydrogel films with a loading efficiency of up to 30%. The drug release characteristics were evaluated, and the profile was fitted by mathematical models that describe the physical processes taking place during the drug transfer from the polymer to a PBS (phosphate-buffered saline) solution. Depending on the design of the polymeric network and the nanofillers included, it was demonstrated that the nafcillin loaded into the nanocomposite hydrogel films ensured a high to moderate activity against S. aureus and S. pyogenes and no activity against E. coli. Furthermore, it was demonstrated that the presence of zinc ions in these polymeric matrices can be correlated with the inactivation of E. coli.
Tensile Behavior of Chain Links Made of Polymeric Materials Manufactured by 3D Printing
Authors: Rădulescu Bruno , Mihalache Andrei Marius , Păduraru Emilian , Hriţuc Adelina , Rădulescu Mara Cristina , Slătineanu Laurenţiu , Ermolai Vasile
Published: July 2023

For reduced mechanical stress, some chains with links made of metallic materials could be replaced by chains made of polymeric materials. A lower weight and a higher corrosion resistance would characterize such chains. From this point of view, research on the behavior of chain links made of polymeric materials under the action of tensile stresses can become important. Modeling by the finite element method highlighted some specific aspects of the behavior of a chain link subjected to tensile stresses. Later, we resorted to the manufacture by 3D printing of some chain links from four distinct polymeric materials, with the modification of the size of the chain link and, respectively, of the values of some of the input factors in the 3D printing process. The tensile strength of the chain links was determined using specialized equipment. The experimental results were processed mathematically to determine some empirical mathematical models that highlight the influence of the values of the input factors in the 3D printing process on the tensile strength of the samples in the form of chain links. It thus became possible to compare the results obtained for the four polymeric materials considered and identify the polymeric material that provides the highest tensile strength of the sample in the form of a chain link. The results of the experimental research showed that the highest mechanical resistance was obtained in the case of the links made of polyethylene terephthalate glycol (PETG). According to experimental results, when tested under identical conditions, PETG links can break for a force value of 40.9 N. In comparison, polylactic acid links will break for a force value of 4.70 N. Links printed in the horizontal position were almost 9-fold stronger than those printed in the vertical position. Under the same test conditions, according to the determined empirical mathematical models, PETG links printed in a horizontal position will break for a force of 300.8 N, while links printed in a vertical position will break for force values of 35.8 N.
Infectious Bronchitis Virus (Gammacoronavirus) in Poultry: Genomic Architecture, Post-Translational Modifications, and Structural Motifs
Authors: Bhuiyan Md. Safiul Alam , Sarker Subir , Amin Zarina , Rodrigues Kenneth Francis , Saallah Suryani , Shaarani Sharifudin Md. , Siddiquee Shafiquzzaman
Published: July 2023

Infectious bronchitis virus (IBV) is an avian coronavirus (CoV) that belongs to the genus Gammacoronavirus and has been listed as an important disease by the World Organization for Animal Health (WOAH). It causes highly contagious respiratory, reproductive, and renal diseases in commercial poultry farms. Multiple IBV serotypes and genotypes have been identified in many countries and many detected variants do not provide cross-protection against infection, resulting in repeated outbreaks and significant economic losses worldwide. In addition, the high genetic mutations and recombination events in the prominent genomic regions of IBV, particularly in the spike glycoprotein (S) and nucleocapsid (N) proteins, are directly involved in the evolutionary processes of IBV and lead to increased pathogenicity and tissue tropism. The characterization of the different genotypes and the relationship between the structure, function, post-translational modifications (PTMs), and structural motifs will elucidate the mechanisms that promote replication and pathogenicity and affect the host’s immune response during infection. In this review, we discuss the molecular features of various IBV genes and proteins that contribute to the infection process. We also highlight the common PTMs and structural motifs that occur during protein synthesis and are essential components of IBV ecology.
Dyes and Heavy Metals Removal from Aqueous Solutions Using Raw and Modified Diatomite
Authors: Muntean Simona , Nistor Maria , Nastas Raisa , Petuhov Oleg
Published: July 2023

The progress of the textile industry has led to a severe increase in the discharge of colored effluents, polluted with dyes and metal ions (non-biodegradable, carcinogenic to humans and environmental hazards). The implementation of effective methodologies and materials for the treatment of wastewater has become an urgent requirement. The present work describes the application of two samples of mineral materials—Ghidirim diatomite and modified diatomite—as adsorbents for the removal of dyes—Acid Blue 350, Methylene Blue, Basic Red 2—and of metal ions—copper, zinc, and lead—from aqueous solutions. In order to determine the optimal working conditions by which to ensure maximum removal efficiency, the influence of the nature and amount of the sorbent, the initial concentration of pollutant, and the temperature were studied. Working under normal conditions (room temperature, solution pH) efficiencies greater than 80% were obtained for the removal of dyes and metal ions. The adsorption fitted well with the pseudo-second order kinetic model, and the maximum adsorption capacities were determined from the Langmuir isotherm model. The adsorption of investigated pollutants is an endothermic and spontaneous process. The results indicate that Ghidirim diatomite and modified diatomite have potential applications in water purification management, providing high removal efficiency of both dyes and metal ions.
Change Characteristics of the Advance Stress and Strata Fracture Structure of Spatial Isolated Island Formed by Roof Drainage
Authors: Li Fenghui , Cheng Yunhai , Zhang Xiufeng , Huang Shunjie , Li Dong
Published: July 2023

Large-energy microseismic events, coal bursts, and other abnormal mine stress are often observed in spatial isolated island areas in the roof water-rich gob-side working face during mining operations. In view of this problem, numerical simulation and on-site microseismic monitoring are employed to study the advance abutment stress and strata fracture structure in the spatial island area formed by ‘roof drainage + goaf’ during mining and the method of eliminating the spatial island area. The results show that the lateral stress is distributed in a step-like pattern, and the drainage area and goaf are superimposed to form a high-stress space island area before the mining of the working face along the goaf. The distribution pattern of the advance abutment stress in the spatial island area changes from ‘C’ type to ‘S’ type during the mining process. The strata structure of the roof water-rich gob-side working face is either a single-peak arch or double-peak arch, and the arch peak corresponds to the boundary of the drainage area. The method of local hydraulic fracturing can eliminate the influence of spatial island areas and improve the safety of roof water-rich gob-side working face mining.
The Evolution of Variance and Entropy of the Granule Size Distribution in Fluidized Bed Agglomeration
Authors: Cronin Kevin , Gutiérrez Ortiz Francisco Javier
Published: July 2023

The evolution of the variance and entropy of granule size in the fluidized bed agglomeration process using two different aggregation kernels is examined. The first is a constant kernel (aggregation is independent of both time and granule size) and permits the most unconstrained agglomeration process that can occur where granules in any size class (up to a maximum size) can be formed at any point in time. This gives the fastest and largest increase in the variance and entropy of the resulting granule size distribution. The second kernel is a mechanistic kernel including a granule growth-limiting mechanism, in this case implemented by the consideration that not all collisions result in coalescence. This markedly changes the evolution of the variance and entropy of the distribution and reduces both significantly. Quantifying the entropy of the distribution provides another perspective on the change in the size distribution in an agglomeration process. It is shown that entropy can provide a better measure of size evolution than variance in that it represents the changing shape of the distribution more closely.
Exergy and Energy Analysis of the Shell-and-Tube Heat Exchanger for a Poultry Litter Co-Combustion Process
Authors: Alamu Samuel O. , Lee Seong W. , Qian Xuejun
Published: July 2023

Increasing production of poultry litter, and its associated problems, stimulates the need for generating useful energy in an environmentally friendly and efficient energy system, such as the use of shell-and-tube heat exchangers (STHE) in a fluidized-bed combustion (FBC) system. A holistic approach which involves the integration of the First Law of Thermodynamics (FLT) and Second Law of Thermodynamics (SLT) is required for conducting effective assessment of an energy system. In this study, the STHE designed by the CAESECT research group, which was integrated into the lab-scale FBC, was investigated to determine the maximum available work performed by the system and account for the exergy loss due to irreversibility. The effects of varying operating parameters and configuration of the space heaters connected to the STHE for space heating purposes were investigated in order to improve the thermal efficiency of the poultry litter-to-energy conversion process. Exergy and energy analysis performed on the STHE using flue gas and water media showed higher efficiency (75–92%) obtained via energy analysis, but much lower efficiency (12–25%) was obtained when the ambient conditions were factored into the exergy analysis, thus indicating huge exergy loss to the surroundings. From the obtained experimental data coupled with the simulation on parallel arrangement of air heaters, it was observed that exergy loss increased with increasing flue gas flow rate from 46.8–57.6 kg/h and with increasing ambient temperature from 8.8 °C to 25 °C. To lower the cost of STHE during final design, a larger temperature difference between the hot and cold flue gas is needed throughout the exchanger, which further increases the exergetic loss while maintaining an energy balance. In addition, this study also found the optimal conditions to reduce exergy loss and improve energy efficiency of the designed STHE. This study shows the possibility to evaluate energy systems using integration of exergy and energy analysis.
Failure Analysis of Casing in Shale Oil Wells under Multistage Fracturing Conditions
Authors: Mou Yisheng , Zhao Han , Cui Jian , Wang Zhe , Wei Fengqi , Han Lihong
Published: July 2023

During the multistage fracturing in shale oil and gas wells with tieback and liner, one of the major challenges is the wellbore temperature variation due to the high-rate fracturing. In such a case, the axial shrinkage trend of the casing string could be caused due to the sudden drop in temperature, but the actual axial length of the casing string would not change due to the cement constraints. Therefore, this could lead to cementation damage between the casing and cement due to excessive load from the casing string. A wellbore seal that is out of control often leads to irreversible consequences, even well abandonment. In order to study the mechanism of casing deformation in shale oil and gas wells with tieback and liner quantitatively, in this paper, take LS1 well (a typical shale oil and gas well with tieback and liner, and casing deformation is caused) for example, the transient changes of temperature and pressure in the whole wellbore during multistage fracturing are studied. Moreover, the cementing strength test of the interface between casing and cement is also tested. Then, the testing results are carried out and extended to model the finite element (FE) model with the whole vertical section casing string with tieback and liner. The model is used to simulate the internal force changes under fracturing conditions with different stages of fracturing. Meanwhile, the casing deformation mechanism in LS1 well is analyzed and studied in detail. Our simulation results indicated the failure process and mechanism of cementation between casing and cement in shale oil and gas wells with tieback and liner. Our work can provide a detailed theoretical reference and a basis for field application.
A Review of Treatment Technologies for Perfluorooctane Sulfonate (PFOS) and Perfluorooctanoic Acid (PFOA) in Water
Authors: Cheng Juntao , Huang Liming , Li Yunfeng , Zhang Zhen , Mu Runzhi , Liu Changqing , Hu Shuncheng , Xiao Yihua , Xu Mengchen
Published: July 2023

Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) are a category of persistent, non-degradable pollutants that are widespread in the environment and in humans. They have attracted considerable attention due to their high bioaccumulation, multiple toxicities, long-term stability, and, in particular, their effects on human health. Therefore, there is an urgent need for highly efficient technologies and systematic mechanisms for the degradation of PFOS and PFOA. Therefore, we summarize four mainstream technologies for the degradation of PFOS and PFOA in water and their research progress in this review, namely adsorption, advanced oxidation processes, microbial treatment, and membrane separation. Among them, adsorption technology is the earliest and relatively mature, the advanced oxidation process has relatively high treatment efficiency, there are deep and broad development prospects for microbial treatment in the future, and membrane separation technology can recycle raw materials and help to save resources. Based on the analysis of the advantages and disadvantages of the existing technologies, the development prospect of reasonable optimization technology is prospected, with a view to providing a practical scientific reference for the research and engineering practice of PFOS and PFOA pollution control in China.
An Advanced Optimization Method to Minimize the Detection Limit of Liquid Scintillation Counter to Measure Low-Level Tritium Activity in Groundwater
Authors: Mamun Al
Published: July 2023

In arid regions, the tritium concentration in groundwater is typically very low and often falls below the minimum detectable activity (MDA) of the conventional liquid scintillation counter (LSC). Therefore, to measure the tritium activity concentration, it is necessary to lower the detection limit so that the scintillation counter can detect it. In the present study, several methods are discussed which are effective at lowering the detectable activity of tritium. One of these methods is to enrich the tritium activity concentration by ten- to fortyfold of the initial concentration of the tritium. Twelve spiked samples with known amounts of tritium, five with high concentrations and seven with low concentrations, were enriched by the electrolysis process. The results indicated that enriching the tritium levels in groundwater lowers the MDA value. Other methods are minimizing background radiation using low-background materials for sample containers, increasing the measurement efficiency of the scintillation counter and counting time, and shielding the sample from environmental radiation using the shutter option in LSC. Moreover, reducing the number of interfering contaminants in the sample can lower the uncertainty in measuring the tritium concentration in the water sample, which is beneficial for detecting low-level tritium in water to ensure public health and safety.
Phenomenology of Language as Praeambula Fidei: A Study Based on Edith Stein
Authors: Ramos Gómez Miriam
Published: July 2023

The 20th century “linguistic turn” seems to have established itself as one of the main defining aspects of contemporary philosophy. This is especially visible in analytic philosophy’s emphasis on the study of language, ultimately disconnecting and isolating it from its deep connection to the person. Without denying the validity and meaning of this type of approach, the aim of this article is to highlight that, in Edith Stein’s work, it is possible to find a phenomenology of language, in which the connection with the totality of the person, with its spiritual dimension, is essential. Although it is true that none of the works by this author systematically deal with questions related to language as such, both in her more strictly phenomenological works as well as in those that are also inspired in the philosophy of Aquinas, we find thought-provoking reflections on the question of language. Once the philosophy of language becomes re-connected to philosophical anthropology, it is transformed by Stein into the praeambula fidei, which enables dialogue with theology, especially with the theology of the body and what Vatican II calls the “ecclesiology of the sign”.
Viewpoint Generation Using Feature-Based Constrained Spaces for Robot Vision Systems
Authors: Magaña Alejandro , Dirr Jonas , Bauer Philipp , Reinhart Gunther
Published: July 2023

The efficient computation of viewpoints while considering various system and process constraints is a common challenge that any robot vision system is confronted with when trying to execute a vision task. Although fundamental research has provided solid and sound solutions for tackling this problem, a holistic framework that poses its formal description, considers the heterogeneity of robot vision systems, and offers an integrated solution remains unaddressed. Hence, this publication outlines the generation of viewpoints as a geometrical problem and introduces a generalized theoretical framework based on Feature-Based Constrained Spaces (C-spaces) as the backbone for solving it. A C-space can be understood as the topological space that a viewpoint constraint spans, where the sensor can be positioned for acquiring a feature while fulfilling the constraint. The present study demonstrates that many viewpoint constraints can be efficiently formulated as C-spaces, providing geometric, deterministic, and closed solutions. The introduced C-spaces are characterized based on generic domain and viewpoint constraints models to ease the transferability of the present framework to different applications and robot vision systems. The effectiveness and efficiency of the concepts introduced are verified on a simulation-based scenario and validated on a real robot vision system comprising two different sensors.
Comprehensive Sustainability Indicator for Land Resource-Carrying Capacity in a Farming-Pastoral Region
Authors: Wang Suizi , Zhang Yaxian , Fan Jiangwen , Zhang Haiyan , Fang Huajun
Published: July 2023

The Northeast Farming-pastoral ecotone (NFPE) in China is crucial for farming and pastoralism. However, sustainable development of land resources faces several challenges from human activities and environmental degradation. Thus, assessing land resource-carrying capacity (LRCC) is crucial for effective land-use planning and management. This study proposes a comprehensive indicator system to evaluate LRCC in the NFPE. We integrate remote sensing, model simulation, and statistical data, employing Geographic Information System analysis and statistical techniques to assess spatiotemporal evolution characteristics of LRCC. Findings indicate increasing LRCC with spatial-temporal heterogeneity. The northern region exhibits relatively high and growing LRCC, while some eastern regions have low LRCC but a high growth trend. The southern region displays both low LRCC and growth. Balancing regional development and ecological protection requires considering spatial heterogeneity of LRCC in land-use planning and management decisions, facilitating differentiated land-use strategies for sustainable development.