bluuebunny/crossref_metadata_2025 huggingface dataset:
The Dynamics of Pheromone Release in Two Passive Dispensers Commonly Used for Mating Disruption in the Control of Lobesia botrana and Eupoecilia ambiguella in Vineyards
Authors: Corbetta Marta , Bricchi Luca , Rossi Vittorio , Fedele Giorgia
Published: December 2024

Background: Mating disruption (MD) is a worthwhile technique for the control of Lobesia botrana and Eupoecilia ambiguella in central Europe and Mediterranean areas. MD efficacy is affected by the pheromone release (PR), which in turn is influenced by environmental conditions. Methods: The effect of weather conditions on PR was evaluated under four different fields in northern Italy. The PR of two commercial types of MD passive dispensers was correlated with different variables. Results: For both dispensers, the temperature and vapor pressure deficit explained PR in vineyards with diverse weather conditions better than time. The effect of temperature was not linear, and any temperature increase at high temperatures accelerated the PRR more than proportionally. One dispenser type showed a non-linear release trend of the pheromone emission in field conditions with respect to the considered variables; further, the stepwise regression pointed out the importance of the dichotomous variable associated with the vineyard for increasing the goodness-of-fit. Conclusions: The equations developed in this work are dispenser-dependent and can provide information on the PR during the season for each dispenser type, as influenced by weather conditions. These equations could serve as an input for a pheromone concentration model to predict concentrations based on meteorological conditions.
Transcriptomic Characterization of Phototransduction Genes of the Asian Citrus Psyllid Diaphorina citri Kuwayama
Authors: Chen Shao-Ping , Chu Xue-Mei , Chi Mei-Xiang , Zhao Jian , Qiu Rong-Zhou
Published: December 2024

Opsin plays a regulatory role in phototaxis of Diaphorina citri, functioning as the initial station in the phototransduction cascade. Our study aimed to explore the D. citri phototransduction pathway to identify elicitors that may enhance D. citri phototaxis in the future. The RNAi technique was employed to inhibit LW-opsin gene expression, followed by RNA-Seq analysis to identify phototransduction genes. Finally, RT-qPCR was performed to validate whether genes in the phototransduction pathway were affected by the inhibition of LW-opsin expression. A total of 87 genes were identified within the transcriptome as involved in phototransduction based on KEGG functional annotation. Of these, 71 genes were identified as enriched in the phototransduction-fly pathway. These genes encode key proteins in this process, including Gqα, Gqβ, Gqγ, phospholipase C β (PLCβ), the cation channel transient receptor potential (TRP), and TRP-like (TRPL), among others. Moreover, the LOC103513214 (Gqβ) and LOC103518375 (ninaC) genes exhibited reduced expression when LW-opsin gene expression was suppressed. Our results provide a basis for further investigation of phototransduction in D. citri.
Multidisciplinary Digital Therapeutics for Chronic Low Back Pain Versus In-Person Therapeutic Exercise with Education: A Randomized Controlled Pilot Study
Authors: Kang Dong-Ho , Park Jae Hyeon , Yoon Chan , Choi Chi-Hyun , Lee Sanghee , Park Tae Hyun , Chang Sam Yeol , Jang Seong-Ho
Published: December 2024

Background: Chronic lower back pain (CLBP) is a global health issue leading to significant disability and socioeconomic burden. Traditional treatments, including exercise and cognitive behavioral therapy (CBT), are often limited by physical and temporal constraints. This study aimed to evaluate the efficacy of multidisciplinary digital therapeutics (MORA Cure LBP) compared to conventional treatments. Methods: This multicenter, randomized, controlled pilot study enrolled 46 participants. Participants were randomly assigned in a 1:1 ratio to either a MORA Cure LBP group or control group, which received conventional treatment. Results: At eight weeks, both groups demonstrated improvements compared to baseline. No statistically significant differences were observed between the MORA Cure LBP and control groups in reductions in usual pain intensity (MORA Cure LBP: 3.1 ± 1.9 vs. control: 3.0 ± 1.5, p = 0.809), worst pain intensity (MORA Cure LBP: 5.00 ± 2.18 vs. control: 4.27 ± 1.83, p = 0.247), and functional disability (ODI, MORA Cure LBP: 15.6 ± 9.6 vs. control: 15.6 ± 10.0, p > 0.999). Compliance was significantly higher in the MORA Cure LBP group during the first 4 weeks (MORA Cure LBP: 74.7% ± 27.4 vs. control: 53.1% ± 28.6, p < 0.001). Conclusions: Both multidisciplinary digital therapeutics (MORA Cure LBP) and conventional treatments were effective in reducing pain and functional disability in patients with CLBP, with no significant differences between the two groups. Digital therapeutics, particularly those that integrate CBT and exercise, offer promising alternatives to conventional therapies by improving accessibility and potentially enhancing patient engagement.
Results of a Codesign Process: A Cognition Screening Pathway for Inpatient and Outpatient Settings for Patients Who Are Facing or Have Undergone Lower Limb Amputation
Authors: Dawes Erinn , Hewitt Lyndel , Bliokas Vida , Wilson Val
Published: December 2024

Background/Objectives: Cognition plays a major role in prosthetic rehabilitation success. The ability to identify patients who may have difficulty understanding and adapting to the rehabilitation process is beneficial for clinicians and patients to allow for targeted and appropriate therapy. The research aim was to codesign a process that facilitates routine cognitive screening into the amputee inpatient journey. Methods: A convenience sample of sixteen medical and allied health practitioners from one local health district undertook a codesign process over 10 months from March to November 2023. A combination of virtual and face-to-face data collection occurred. Each of the codesign meetings was audio recorded, following which transcription occurred. Transcripts were reviewed using thematic analysis-based techniques to capture themes and consensus within the group. Results: Two pathways were established for use within one local health district, allowing clinicians to measure the cognition of patients in both inpatient and outpatient settings either before or after they underwent amputation. The newly established pathways provide step-by-step guidance for clinicians, such as how to address contraindicators for testing and providing guidance for subsequent neuropsychological testing. The Montreal Cognitive Assessment (MoCA), both paper based and electronic based, was selected as the cognitive screening tool for implementation. Conclusions: Utilizing codesign as a method for generating a cognitive screening pathway for amputees was successful. The pathways generated should be reviewed for suitability for application in other health settings.
Successful Treatment of an Esophago-Tracheobronchial Fistula Using Double Stenting to Correct Initial Stent Migration: A Case Report and Literature Review
Authors: Nishio Yuya , Matsuo Hideki , Nagamatsu Shinsaku , Shioya Kazuki , Yamamoto Chisa , Kikukawa Shoma , Matsuura Kyohei , Fujimoto Yuki , Uejima Masakazu , Moriya Kei
Published: December 2024

Background: Esophago-tracheobronchial fistula is a severe and often fatal complication in patients with advanced esophageal cancer, requiring prompt attention. The standard treatment involves the placement of a covered stent, which is relatively simple to perform and effectively seals the fistula. However, stent migration remains a common issue, highlighting the need for improved methods to prevent it. Case Presentation: We developed an innovative double stenting method approach utilizing two types of metal stents for cases where conventional stenting led to early stent dislodgement. This technique combines the benefits of uncovered and fully covered stents while minimizing their limitations. Furthermore, it is straightforward and adaptable. In two cases treated at our facility, this method successfully maintained complete fistula coverage until the patients’ deaths, allowing them to consume food orally. Conclusions: Here, we describe the procedure in detail and discuss its significance, as our findings demonstrate the effectiveness of the double stenting technique.
Management of Postpartum Hemorrhage in Low- and Middle-Income Countries: Emergency Need for Updated Approach Due to Specific Circumstances, Resources, and Availabilities
Authors: Aimagambetova Gulzhanat , Bapayeva Gauri , Sakhipova Gulnara , Terzic Milan
Published: December 2024

Postpartum hemorrhage (PPH) represents a critical emergency condition and the principal cause of maternal morbidity and mortality worldwide. It encompasses excessive bleeding following childbirth, which can arise from various causes. Prompt recognition and management are essential to mitigate severe outcomes and ensure maternal safety. The incidence of PPH in low- and middle-income countries (LMICs) is higher than in developed countries. Healthcare systems in developing countries face multiple challenges that may impact PPH management at policy, facility, and community levels. The mentioned barriers could be addressed by providing an empowering environment via the implementation of supportive policies, access to PPH care, planning supplies, allying strategies, providing training, and utilization of guidelines and algorithms for PPH management. Evidence-based international guidelines should serve as an integral part of appropriate management. On the other hand, LMICs have limited opportunities to implement the proposed international algorithms and guidelines. Therefore, some amendments based on the resource/expertise availability should be considered at the specific clinical site. This review summarizes and updates the accumulated knowledge on postpartum hemorrhage, focusing on challenging management options in developing countries. In many LMICs, maternal morbidity and mortality linked to PPH were improved after the implementation of standardized protocols and timely and purposeful interventions. International support in healthcare professionals’ training, enhancing resources, and the provision of an adapted evidence-based approach could assist in improving the management of PPH in LMICs. Refining our understanding of specific local circumstances, international support in specialists’ training, and the provision of evidence-based approaches may assist in improving the management of PPH in LMICs and contribute to safer childbirth.
Exogenous Ketones in Cardiovascular Disease and Diabetes: From Bench to Bedside
Authors: Kansakar Urna , Nieves Garcia Crystal , Santulli Gaetano , Gambardella Jessica , Mone Pasquale , Jankauskas Stanislovas S. , Lombardi Angela
Published: December 2024

Ketone bodies are molecules produced from fatty acids in the liver that act as energy carriers to peripheral tissues when glucose levels are low. Carbohydrate- and calorie-restricted diets, known to increase the levels of circulating ketone bodies, have attracted significant attention in recent years due to their potential health benefits in several diseases. Specifically, increasing ketones through dietary modulation has been reported to be beneficial for cardiovascular health and to improve glucose homeostasis and insulin resistance. Interestingly, although excessive production of ketones may lead to life-threatening ketoacidosis in diabetic patients, mounting evidence suggests that modest levels of ketones play adaptive and beneficial roles in pancreatic beta cells, although the exact mechanisms are still unknown. Of note, Sodium-Glucose Transporter 2 (SGLT2) inhibitors have been shown to increase the levels of beta-hydroxybutyrate (BHB), the most abundant ketone circulating in the human body, which may play a pivotal role in mediating some of their protective effects in cardiovascular health and diabetes. This systematic review provides a comprehensive overview of the scientific literature and presents an analysis of the effects of ketone bodies on cardiovascular pathophysiology and pancreatic beta cell function. The evidence from both preclinical and clinical studies indicates that exogenous ketones may have significant beneficial effects on both cardiomyocytes and pancreatic beta cells, making them intriguing candidates for potential cardioprotective therapies and to preserve beta cell function in patients with diabetes.
The Clinical and Radiological Outcomes and Complications of Bilboquet Implant for Proximal Humerus Fractures: A Systematic Review
Authors: Samargandi Ramy , Albashri Jawad , Albashri Ahmed , Alzahrani Faris , Hassan Abdulrahman , Berhouet Julien
Published: December 2024

Background/Objectives: There is no consensus in the literature regarding the optimal treatment for complex proximal humerus fractures (PHFs). The aim of this study is to evaluate the clinical, functional, and radiological outcomes, and complications, associated with the Bilboquet implant in the treatment of PHFs. Methods: The search was conducted from the first description of the Bilboquet device in 1994 to June 2024, across PubMed, Web of Science, and Google Scholar, using specific keywords such as (“Bilboquet” OR “Bilboquet prosthesis” OR “Bilboquet device”) AND (“proximal humerus fracture” OR “shoulder fracture”), along with Boolean operators. The inclusion criteria comprised studies published in English or French that focused on the use of the Bilboquet implant for PHFs. Eligible study designs included case series, cohort studies, randomized controlled trials (RCTs), and non-RCTs evaluating clinical, functional, and radiological outcomes, and complications. Studies that do not contain relevant results to this systematic review, pediatric populations, or the use of alternative implants were excluded. Results: A total of eight studies (235 patients) published between 1996 and 2021 were included. The mean age was 68.6 years (56 to 76.8) in all the studies. The majority of patients, 76.2%, were females, with male patients accounting for only 23.8%. A total of 10 (4.3%) patients had 2-part fractures, 40% of patients had 3-part fractures, and 55.7% of patients had 4-part fractures. The mean follow-up was 36.4 months (25.8–88.7), with a mean constant score of 69.7 (62–78.6). Complications included non-union in 2.65% of cases, avascular necrosis in 19.7%, revision surgery in 5.1%, and protrusion of the staple in 4.3%. Conclusions: Despite limited knowledge of the Bilboquet implant, it shows promise in managing complex PHFs in both young and older adults, with favorable clinical and radiological outcomes. It offers advantages over traditional fixation methods and allows easy conversion to arthroplasty if osteonecrosis occurs. However, the long-term outcomes require further study. While early results are promising, larger randomized studies are needed to confirm its broader clinical utility.
A Comparison Study of High-Temperature Low-Cycle Fatigue Behaviour and Deformation Mechanisms Between Incoloy 800H and Its Weldments
Authors: Li Wenjing , Xiao Lin , Walters Lori , Kasprick Greg , Sloan Robyn
Published: December 2024

The high-temperature low-cycle fatigue (LCF) behaviour of Incoloy 800H and its weldments with Haynes 230 and Inconel 82 filler metals, which were fabricated with the gas tungsten arc welding (GTAW) technique, was investigated and compared at 760 °C. The results revealed that the Incoloy 800H weldments showed lower fatigue lifetimes compared to the base metal. However, the weldments with the Haynes 230 filler metal demonstrated an improved fatigue life at the low strain amplitude compared to both Incoloy 800H and the weldment with the Inconel 82 filler metal. The Incoloy 800H base metal showed pronounced initial cyclic hardening with hardening factors increasing with strain amplitudes. In contrast, the weldments with Haynes 230 and Inconel 82 filler metals displayed short initial cyclic hardening and saturation stages, followed by long continuous cyclic softening. The fractography and microstructure after LCF the tests were characterized with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Transgranular fracture with multiple crack initiations was the predominant failure mode on the fracture surfaces of both Incoloy 800 base metal and the weldments. TEM examination revealed that planar dislocation slips at the low strain amplitude evolved to wavy slips, eventually forming a cell structure at high strain amplitudes in the Incoloy 800H material as the strain amplitudes increased. However, the weld metal exhibited a planar slip mode deformation mechanism regardless of cyclic strain amplitude in the weldment specimens. The differing cyclic hardening and softening behaviours between Incoloy 800H and its weldments are attributed to the higher strength of the weldment specimens compared to the base metal. In the Incoloy 800H base material specimens, the reverse strains during LCF created wavy dislocation structures, which could not fully recover due to the non-reversible nature of the microstructure. As a result, cells or subgrains formed within the microstructure once created. In contrast, the higher strength of the weld metal in the weldment specimens significantly suppressed the formation of wavy dislocation structures, and deformation primarily manifested as planar arrays of dislocations.
Parasitic Protozoa and Other Vector-Borne Pathogens in Captive Mammals from Brazil
Authors: Castillo Anisleidy Pérez , Colácio Nicolas , Rodrigues Pedro Henrique Cotrin , Miranda João Victor Oliveira , Lima Paula Cristina Senra , Motta Rafael Otávio Cançado , Tinoco Herlandes Penha , Coelho Carlyle Mendes , da Silveira Júlia Angélica Gonçalves
Published: December 2024

In captive environments, mammals are frequently exposed to various parasitic protozoa and other vector-borne pathogens that can impact both animal health and public health. Monitoring these pathogens is essential for animal welfare and zoonotic disease control. This study aimed to investigate the prevalence of parasitic protozoa and other vector-borne pathogens in captive mammals through molecular detection methods at the Belo Horizonte Zoo, Brazil. Between November 2021 and March 2023, whole blood samples were collected from 40 mammals. Molecular analyses identified piroplasms, Leishmania spp., granulocytic/platelet Anaplasma/Ehrlichia spp., monocytic Ehrlichia spp., Bartonella spp. and hemotropic Mycoplasma spp. with a 72.5% positivity rate. Piroplasms were found in 22.5% (two Pantanal cats, two gorillas, one white rhinoceros, one spider monkey, one jaguar, one tufted capuchin and one hippo) and Leishmania spp. in 12.9% (four maned wolves). Granulocytic/platelet Anaplasma/Ehrlichia spp. were found in 12.5% of the samples (one gorilla and four maned wolves), Ehrlichia canis in 2.5% of the animals (one maned wolf), Bartonella spp. in 42.5% (six howler monkeys, two maned wolves, one gorilla, one white rhino, one southern tamandua, one common woolly monkey, one tufted capuchin, one brown brocket deer, one agouti, one cougar and one hippo), hemotropic Mycoplasma spp. in 17.5% (one gorilla, one maned wolf, one white rhino, one howler monkey, two common woolly monkeys and one European fallow deer). Five Artiodactyla members tested negative for A. marginale. Coinfections occurred in 34.5% of the positive samples. Sequencing revealed that Theileria spp. and Cytauxzoon spp. are closely related to Theileria bicornis and Cytauxzoon felis; Ehrlichia canis and Bartonella spp. are closely related to B. clarridgeiae and B. henselae; and hemotropic Mycoplasma spp. are closely related to Candidatus Mycoplasma haemominutum. Our results showed a high occurrence of vector-borne pathogens in captive animals, including zoonotic species, which may pose a risk to animal and human public health.
IgA Nephropathy: What Is New in Treatment Options?
Authors: Scarpioni Roberto , Valsania Teresa
Published: December 2024

IgA nephropathy (IgAN), first described in 1968, is one of the most common forms of glomerulonephritis and can progress to end-stage kidney disease (ESKD) in 25 to 30 percent of patients within 20 to 25 years from the onset. It is histologically characterized by mesangial proliferation with prominent IgA deposition. The prognosis may be difficult to predict, but important risk factors for disease progression of kidney disease have been recognized: usually proteinuria above 0.75–1 g/day with or without hematuria, hypertension, high-risk histologic features (such as crescent formation, immune deposits in the capillary loops, mesangial deposits, glomerulosclerosis, tubular atrophy, interstitial fibrosis, and vascular disease), and a reduced Glomerular Filtration Rate (GFR). In the absence of reliable specific biomarkers, current standards of care are addressed to decrease proteinuria, as a surrogate endpoint, and control blood pressure. For a long time, corticosteroids have been considered the only cure for proteinuric patients or those at risk of progression to ESKF; however, unfortunately, like other immunosuppressive agents, they are burdened with high collateral risks. Therefore, optimal treatment remains a challenge, even if, to date, clinicians have many more options available. Here, we will review the main therapies proposed, such as the stronghold of RAAS inhibition and the use of SGLT2 inhibitors; it is expected that ongoing clinical trials may find other therapies, apart from corticosteroids, that may help improve treatment, including both immunosuppressive monoclonal antibodies and other strategies. At the current time, there are no disease-specific therapies available for IgAN, because no largescale RCTs have demonstrated a reduction in mortality or in major adverse kidney or cardiovascular events with any therapy.
The Role of Trust in Facilitating Land Transfers and Contract Formalization: A Study of Chinese Farm Households
Authors: Xie Dongying , Xu Mengbo
Published: December 2024

Facilitating land transfers and contract standardization is crucial for optimizing farmland use and achieving high-quality agricultural development following the round of confirmation, registration, and certification of contracted rural land management rights by the government. Social trust is crucial for cracking the growth dilemma of land transfer, and the impact of farmers’ trust on land transfer may also change after the round of land titling. This study aims to examine the impact of social trust on farm households’ land transfer decisions and the formalization of land transfer contracts within the context of the new round of land titling in China, using models such as IV-Probit, Heckprobit, and conditional mixed process (CMP), with survey data from 2600 Chinese farmers in 2020–2021. The results show that increased social trust significantly enhances both land transfer and contract formalization, and these results remain robust after addressing potential endogeneity. Further analysis reveals that while interpersonal trust promotes land transfer, institutional trust plays a more pivotal role in the formalization of contracts. The results underscore the importance of strengthening grassroots governance to foster institutional trust, enhance institutional reliability, and support the regulated growth of the land transfer market.
Genome-Wide Identification and Characterization of OSC Gene Family in Gynostemma pentaphyllum (Cucurbitaceae)
Authors: Zhang Xiao , Yang Huan , Wang Xuan , Wang Xiaoting , Chen Chen
Published: December 2024

Gynostemma pentaphyllum is a traditional Chinese medicinal plant of considerable application value and commercial potential, primarily due to its production of various bioactive compounds, particularly dammarane-type triterpenoid saponins that are structurally analogous to ginsenosides. Oxidosqualene cyclase (OSC), a pivotal enzyme in the biosynthesis of triterpenoid metabolites in plants, catalyzes the conversion of oxidosqualene into triterpenoid precursors, which are essential components of the secondary metabolites found in G. pentaphyllum. To elucidate the role of OSC gene family members in the synthesis of gypenosides within G. pentaphyllum, this study undertook a comprehensive genome-wide identification and characterization of OSC genes within G. pentaphyllum and compared their expression levels across populations distributed over different geographical regions by both transcriptome sequencing and qRT-PCR experimental validation. The results identified a total of 11 members of the OSC gene family within the genome of G. pentaphyllum. These genes encode proteins ranging from 356 to 767 amino acids, exhibiting minor variations in their physicochemical properties, and are localized in peroxisomes, cytoplasm, plasma membranes, and lysosomes. All GpOSCs contain highly conserved DCTAE and QW sequences that are characteristic of the OSC gene family. A phylogenetic analysis categorized the GpOSCs into four distinct subfamilies. A cis-element analysis of the GpOSC promoters revealed a substantial number of abiotic stress-related elements, indicating that these genes may respond to drought conditions, low temperatures, and anaerobic environments, thus potentially contributing to the stress resistance observed in G. pentaphyllum. Expression analyses across different G. pentaphyllum populations demonstrated significant variability in OSC gene expression among geographically diverse samples of G. pentaphyllum, likely attributable to genetic variation or external factors such as environmental conditions and soil composition. These differences may lead to the synthesis of various types of gypenosides within geographically distinct G. pentaphyllum populations. The findings from this study enhance our understanding of both the evolutionary history of the OSC gene family in G. pentaphyllum and the biosynthetic mechanisms underlying triterpenoid compounds. This knowledge is essential for investigating molecular mechanisms involved in forming dammarane-type triterpenoid saponins as well as comprehending geographical variations within G. pentaphyllum populations. Furthermore, this research lays a foundation for employing plant genetic engineering techniques aimed at increasing gypenoside content.
Artificial Intelligence in the Non-Invasive Detection of Melanoma
Authors: İsmail Mendi Banu , Kose Kivanc , Fleshner Lauren , Adam Richard , Safai Bijan , Farabi Banu , Atak Mehmet Fatih
Published: December 2024

Skin cancer is one of the most prevalent cancers worldwide, with increasing incidence. Skin cancer is typically classified as melanoma or non-melanoma skin cancer. Although melanoma is less common than basal or squamous cell carcinomas, it is the deadliest form of cancer, with nearly 8300 Americans expected to die from it each year. Biopsies are currently the gold standard in diagnosing melanoma; however, they can be invasive, expensive, and inaccessible to lower-income individuals. Currently, suspicious lesions are triaged with image-based technologies, such as dermoscopy and confocal microscopy. While these techniques are useful, there is wide inter-user variability and minimal training for dermatology residents on how to properly use these devices. The use of artificial intelligence (AI)-based technologies in dermatology has emerged in recent years to assist in the diagnosis of melanoma that may be more accessible to all patients and more accurate than current methods of screening. This review explores the current status of the application of AI-based algorithms in the detection of melanoma, underscoring its potential to aid dermatologists in clinical practice. We specifically focus on AI application in clinical imaging, dermoscopic evaluation, algorithms that can distinguish melanoma from non-melanoma skin cancers, and in vivo skin imaging devices.
Special Issue: “Novel Diagnosis and Treatment of Gastrointestinal Disease”
Authors: Georgescu Doina , Bratosin Felix
Published: December 2024

Significant advances in the diagnosis and treatment of gastrointestinal diseases have been made in recent years [...]
Regulation of Plant Growth and Development by Melatonin
Authors: Shi Dawei , Zhao Lejia , Zhang Ruijia , Song Qiaofeng
Published: December 2024

Melatonin is a naturally occurring chemical with pleiotropic effects in various species. In plants, melatonin is associated with a variety of plant physiological processes, including plant growth and development, stress responses, etc. Thus, melatonin may hold promise for improving crop yields and agricultural sustainability. This review describes the biosynthetic mode of melatonin and its properties and summarizes its functions in growth, development, and reproduction. In addition, the role of melatonin in plants facing various stressful environments is elaborated upon, and its relationship with other phytohormones is summarized. Through this review, we recognize the problems and challenges facing melatonin research and propose some feasible solutions.
Crystal Structures of 9,9-Disubstituted Fluorene Derivatives Bearing Methyl, Hydroxymethyl or Pyridinylmethyl Groups
Authors: Hanauer Thomas , Seidel Pierre , Seichter Wilhelm , Mazik Monika
Published: December 2024

The wide range of application possibilities of fluorene derivatives, due to their unique physical and chemical properties, has made this class of molecules important for various disciplines. The synthesis of these compounds and the study of their properties are, therefore, of great scientific interest. Due to their reactivity and sensitivity to oxidation, the 9-position of the fluorene skeleton is often double substituted, for example, to ensure the long-term stability of materials. Single crystal structure analysis, among other methods, plays a very important role in the development of various fluorene-based systems. In this paper, the crystal structures of three 9,9-disubstituted fluorene derivatives bearing methyl, hydroxymethyl or pyridinylmethyl groups are described (three solvent-free structures and one toluene solvate). A detailed evaluation of the intermolecular interactions, supported by Hirshfeld surface analysis, is the subject of this article.
Magnetic Performance and Anticorrosion Coating Stability of Thermally Demagnetized Nd-Fe-B Permanent Magnets for Reuse Applications
Authors: Tomše Tomaž , Kubelka Pierre , Moreno López Rosario , Fleissner Peter , Grau Laura , Zaplotnik Matej , Burkhardt Carlo
Published: December 2024

Nd-Fe-B-type permanent magnets, containing approximately 30% critical rare-earth elements by weight, are essential components in renewable energy systems (e.g., wind turbines, hydroelectric generators) and electric vehicles. They are also critical for consumer electronics and electric motors in products like energy-efficient air conditioners and home appliances. In light of advancing sustainability goals, the direct reuse of magnets from end-of-life devices offers a promising alternative to energy-intensive and costly recycling methods based on hydro- and pyrometallurgical processes, as well as modern short-loop recycling through hydrogen processing. However, Nd-Fe-B magnets must be demagnetized before they can be extracted from devices. This study explores the effects of thermal demagnetization, performed either in air or a vacuum, on the stability of anticorrosion coatings and the magnetic performance of remagnetized magnets. Corrosion tests were conducted to assess the compatibility of various coatings with thermal demagnetization, identifying those most suitable for future applications involving magnet reuse.
The Effect of μ-Limestone Additions on the Mechano-Chemical and Microstructural Properties of Slag and Binary Slag/Ground Fly Ash Alkaline-Activated Binders
Authors: Vázquez-Rodríguez Francisco Javier , López-Yépez Lucio Guillermo , Elizondo-Villarreal Nora , Guzmán-Hernández Ana María , Gómez-Zamorano Lauren Yolanda , Rodríguez-Castellanos Edén Amaral
Published: December 2024

An alternative approach to reducing the clinker factor, i.e., worldwide CO2 emissions resulting from the production of composite cement, is to replace these materials with supplementary aluminosilicate-based materials that promote the formation of alkali-activated cements, whose elevated temperature resistance, limited permeability, strong binding properties, excellent durability, high chemical corrosion resistance, confinement of toxic waste, and environmentally low impact have attracted a lot of attention in the cement industry. The principal aluminosilicate-based supplementary materials (SCMs) used in the cement industry are fly ash and blast-furnace slag. Recently, limestone has been proposed for use in alkali-activated cement to improve mechanical resistance and promote nucleation sources for the hydration of hybrid gels. In the current research work, the effect of 5 and 10 wt% limestone additions to slag and fly ash/slag alkali-activated cements with NaOH-4M was studied to evaluate the mechano-chemical and microstructural properties of alkali-activated cement. The effect of limestone was studied using mechanical resistance, XRD, FTIR, SEM-EDS, and calorimetry methods. The XRD, FTIR, and SEM-EDS results demonstrated the formation of portlandite Ca(OH)2 after the activator solution’s reaction with limestone. The limestone’s dissolution in Ca2+ contributes to hybrid gel formation ((N, C)-A-S-H, N-A-S-H, and C-A-S-H), resulting in compressive strength higher than 20 MPa, the recommended resistance for commercial cement.
Adaptive Impedance Control of Multirotor UAV for Accurate and Robust Path Following
Authors: Ahmed Zain , Xiong Xiaofeng
Published: December 2024

Unmanned Aerial Vehicles (UAVs) have become essential tools in various industries for tasks such as inspection, maintenance, and surveillance. An Online Impedance Adaptive Controller (OIAC) is proposed for the online modulating of UAV control gains to obtain better performance and stability of tracking curved trajectories than the traditional methods, Model Reference Adaptive Controller (MRAC) and Proportional–Integral–Derivative (PID). Two UAV path planners with minimal jerk and snap were integrated into OIAC, MRAC, and PID. These six controllers were implemented and compared in a simulated UAV with perceptional noise, which follows curved pipelines and avoids obstacles. Experimental results show that the OIAC controller achieves at least an 80% improvement over the PID controller across all trajectory types in terms of the trajectory tracking error. Additionally, OIAC demonstrates an over 20% improvement in jerk trajectories and a more than 30% improvement in snap trajectories when compared to the MRAC controller. These results indicate that OIAC offers enhanced trajectory tracking accuracy and robustness against perceptual noise. Our work presents an advanced controller of a UAV and its preliminary validation in accurate and robust path tracking.
Revealing the Potential of Fucus vesiculosus Linnaeus for Cosmetic Purposes: Chemical Profile and Biological Activities of Commercial and Wild Samples
Authors: Muñoz-Quintana Marina , Padrón-Sanz Carolina , Dolbeth Marina , Arenas Francisco , Vasconcelos Vitor , Lopes Graciliana
Published: December 2024

The natural products industry is gaining increasing interest, not only due to modern lifestyles becoming more aware of environmental and sustainability issues but also because of the loss of efficacy and undesirable side effects of synthetic ingredients. This pioneering study provides a comprehensive comparison between extracts obtained from wild and commercial samples of Fucus vesiculosus Linnaeus, highlighting their multifaceted benefits in cosmetic applications. The antiaging potential of acetone (70 and 90%) and ethanol 60% extracts from wild and commercial samples of F. vesiculosus, focusing on their application in cosmetics, was explored. The extracts were chemically characterized, their carotenoid profiles being established by HPLC, and the total phenolic content and phlorotannins by spectrophotometry. The extracts were evaluated for their antioxidant potential against the physiologic free radicals superoxide anion radical (O2•−) and nitric oxide (•NO), for their ability to inhibit the enzymes hyaluronidase and tyrosinase, and for their anti-inflammatory potential in the macrophage cell model RAW 264.7. The acetone 70% extract of wild F. vesiculosus was the richest in fucoxanthin, which accounted for more than 67% of the total pigments identified, followed by the acetone 90% extract of the same sample, where both fucoxanthin and pheophytin-a represented 40% of the total pigments. The same behavior was observed for phenolic compounds, with the ethanol 60% presenting the lowest values. A chemical correlation could be established between the chemical composition and the biological activities, with acetone extracts from the wild F. vesiculosus, richer in fucoxanthin and phlorotannins, standing out as natural ingredients with anti-aging potential. Acetone 90% can be highlighted as the most effective extraction solvent, their extracts presenting the highest radicals scavenging capacity, ability to inhibit tyrosinase to a greater extent than the commercial ingredient kojic acid, and potential to slow down the inflammatory process.
Cytotoxic Effects of ZnO and Ag Nanoparticles Synthesized in Microalgae Extracts on PC12 Cells
Authors: Fais Giacomo , Sidorowicz Agnieszka , Perra Giovanni , Dessì Debora , Loy Francesco , Lai Nicola , Follesa Paolo , Orrù Roberto , Cao Giacomo , Concas Alessandro
Published: December 2024

The green synthesis of silver (Ag) and zinc oxide (ZnO) nanoparticles (NPs), as well as Ag/Ag2O/ZnO nanocomposites (NCs), using polar and apolar extracts of Chlorella vulgaris, offers a sustainable method for producing nanomaterials with tunable properties. The impact of the synthesis environment and the nanomaterials’ characteristics on cytotoxicity was evaluated by examining reactive species production and their effects on mitochondrial bioenergetic functions. Cytotoxicity assays on PC12 cells, a cell line originated from a rat pheochromocytoma, an adrenal medulla tumor, demonstrated that Ag/Ag2O NPs synthesized with apolar (Ag/Ag2O NPs A) and polar (Ag/Ag2O NPs P) extracts exhibited significant cytotoxic effects, primarily driven by Ag+ ion release and the disruption of mitochondrial function. However, it is more likely the organic content, rather than size, influenced anticancer activity, as commercial Ag NPs, despite smaller crystallite sizes, exhibit less effective activity. ZnO NPs P showed increased reactive oxygen species (ROS) generation, correlated with higher cytotoxicity, while ZnO NPs A produced lower ROS levels, resulting in diminished cytotoxic effects. A comparative analysis revealed significant differences in LD50 values and toxicity profiles. Differentiated PC12 cells showed higher resistance to ZnO, while AgNPs and Ag/Ag2O-based materials had similar effects on both cell types. This study emphasizes the crucial role of the synthesis environment and bioactive compounds from C. vulgaris in modulating nanoparticle surface chemistry, ROS generation, and cytotoxicity. The results provide valuable insights for designing safer and more effective nanomaterials for biomedical applications, especially for targeting tumor-like cells, by exploring the relationships between nanoparticle size, polarity, capping agents, and nanocomposite structures.
Challenges and Perspectives Regarding the Determination of Gingival Crevicular Fluid Biomarkers During Orthodontic Treatment: A Narrative Review
Authors: Bud Anamaria , Lazăr Luminița , Mârțu Maria-Alexandra , Dakó Timea , Suciu Mircea , Vlasiu Andreea , Lazăr Ana-Petra
Published: December 2024

Background: Changes in the positions of teeth occur during orthodontic treatment due to the application of forces that cause restructuring of the periodontal tissue. In the last decade, substantial research has been conducted to detect different biomarkers in the gingival crevicular fluid (GCF) to obtain a better assessment of the periodontal status. Aim: The purpose of this review is to describe how the levels of certain biomarkers from the gingival fluid change during tissue remodeling throughout orthodontic treatment. Materials and methods: To carry out the purpose of this research, electronic databases were searched using specific keywords, leading to 387 articles, out of which 19 were used in writing this narrative review. A sampling period of the last 10 years was used in selecting the articles. Results: The results highlight that the origin of the gingival crevicular fluid is at the gingival blood vessels’ plexus. GCF has a complex composition with differences depending on the periodontal status and the tissue restructuring which takes place in the periodontium. The levels of inflammatory mediators, enzymes, and metabolic products of tissue remodeling in GCF change during orthodontic treatment. Being aware of their specific role, they can provide valuable information about bone remodeling during orthodontic tooth movement. Conclusions: By determining the biomarkers in GCF, as an investigative method, clinicians could easily monitor the orthodontic tooth movement, and, subsequently, the treatment period could be shortened and the adverse effects associated with it could be avoided.
BDSF Analogues Inhibit Quorum Sensing-Regulated Biofilm Production in Xylella fastidiosa
Authors: Horgan Conor , Baccari Clelia , O’Driscoll Michelle , Lindow Steven E. , O’Sullivan Timothy P.
Published: December 2024

Xylella fastidiosa is an aerobic, Gram-negative bacterium that is responsible for many plant diseases. The bacterium is the causal agent of Pierce’s disease in grapes and is also responsible for citrus variegated chlorosis, peach phony disease, olive quick decline syndrome and leaf scorches of various species. The production of biofilm is intrinsically linked with persistence and transmission in X. fastidiosa. Biofilm formation is regulated by members of the Diffusible Signal Factor (DSF) quorum sensing signalling family which are comprised of a series of long chain cis-unsaturated fatty acids. This article describes the evaluation of a library of N-acyl sulfonamide bioisosteric analogues of BDSF, XfDSF1 and XfDSF2 for their ability to control biofilm production in X. fastidiosa. The compounds were screened against both the wild-type strain Temecula and an rpfF* mutant which can perceive but not produce XfDSF. Planktonic cell abundance was measured via OD600 while standard crystal violet assays were used to determine biofilm biomass. Several compounds were found to be effective biofilm inhibitors depending on the nature of the sulfonamide substituent. The findings reported here may provide future opportunities for biocontrol of this important plant pathogen.
Antibacterial, Antifungal, Antiviral Activity, and Mechanisms of Action of Plant Polyphenols
Authors: Davidova Slavena , Galabov Angel S. , Satchanska Galina
Published: December 2024

This review describes the enhanced classification of polyphenols into flavonoids, lignans, phenolic acids, stilbenes, and tannins. Its focus is the natural sources of polyphenols and an in-depth discussion of their antibacterial, antifungal, and antiviral activity. Besides a broad literature overview, this paper contains authors’ experimental data according to some daily consumed vegetables such as tomatoes, different varieties of onion, garlic, parsley, and cayenne pepper and the probable relation of these activities to polyphenols. The isolation of polyphenols via conventional and ultrasonic, pressurized liquids and pulse-field extractions, as well as their methods for detection and determination, are interpreted as well. The main mechanisms by which polyphenols inhibit the growth of bacteria, fungi, and viruses, such as protein synthesis, cell membrane destabilization, and ROS production induction, are in focus. Data on polyphenol concentrations and their respective MIC or the inhibition zone diameters of different bacterial and fungal species and suppressing viral replication are depicted. The toxicity of polyphenols in vitro, ex vivo, and in vivo towards microorganisms and human/animal cells, and the safety of the polyphenols applied in clinical and industrial applications are expanded. This review also characterizes the antimicrobial effects of some chemically synthesized polyphenol derivatives. Biotechnological advances are also reported, especially the entrapment of polyphenols in biocompatible nanoparticles to enhance their bioavailability and efficacy. Polyphenols are promising for exploring molecules’ novel antimicrobial substances and paving the path for effective novel antimicrobial agents’ discovery, taking into consideration their positives and negatives.
Cholera Outbreaks in Low- and Middle-Income Countries in the Last Decade: A Systematic Review and Meta-Analysis
Authors: Asantewaa Anastasia A. , Odoom Alex , Owusu-Okyere Godfred , Donkor Eric S.
Published: December 2024

Cholera is linked to penury, making low- and middle-income countries (LMICs) particularly vulnerable to outbreaks. In this systematic review, we analyzed the drivers contributing to these outbreaks, focusing on the epidemiology of cholera in LMICs. This review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and was registered in PROSPERO (ID: CRD42024591613). We searched PubMed, Scopus, Web of Science, and Google Scholar to include studies on cholera outbreaks that occurred in LMICs from 1 January 2014 to 21 September 2024. Studies on outbreaks outside LMICs and focusing on sporadic cases were excluded. The risk of bias among included studies was assessed using a modified Downes et al. appraisal tool. Thematic analysis was used to synthesize the qualitative data, and meta-analyses to estimate the pooled prevalence. From 1662 records, 95 studies met inclusion criteria, primarily documenting outbreaks in Africa (74%) and Asia (26%). Contaminated water was the main route of disease transmission. The pooled fatality prevalence was 1.3% (95% CI: 1.1–1.6), and the detection rate among suspected cases was 57.8% (95% CI: 49.2–66.4). Vibrio cholerae O1 was the dominant serogroup while Ogawa was the dominant serotype. All studies reporting biotypes indicated El Tor. Although the isolates were 100% susceptible to ofloxacin, levofloxacin, norfloxacin, cefuroxime, and doxycycline, they were also fully resistant to amikacin, sulfamethoxazole, trimethoprim, and furazolidone. The persistence of cholera outbreaks in destitute areas with limited access to clean water and sanitation emphasizes the need for socioeconomic improvements, infrastructure development, and ongoing surveillance to support timely responses and achieve long-term prevention.
Examination of Sex-Related Differences in Fatigability and Frequency Components of Mechanomyographic Signals During Sustained Exercise
Authors: Benitez Brian , Kwak Minyoung , Succi Pasquale J. , Mitchinson Clara J. , Bergstrom Haley C.
Published: December 2024

Background: Surface mechanomyographic (sMMG) signals have been used to examine sex-specific differences in the mechanical behavior of muscle during fatiguing exercise. However, studies often utilize simple amplitude- and frequency-based analyses, which only reveal the static components of the sMMG signal. Methods: Thus, a wavelet-based analysis was used to examine changes in the spectral intensity of the non-dominant limb’s vastus lateralis during a fatiguing, maximal, unilateral isometric leg extension in recreationally active men (n = 11) and women (n = 10). Relative changes in spectral intensities and instantaneous mean frequency (IMF) were examined using linear mixed-effect models. Time-to-task failure was compared using an independent sample t-test. Results: The neuromuscular responses demonstrated parallel decreases in IMF (p < 0.001). Further, there were parallel, nonlinear, decreases in spectral intensity across wavelets (p < 0.001) and there were no sex differences in time-to-task failure (p = 0.15). Conclusions: These data showed no sex-specific differences in exercise fatigability or muscle mechanics during fatiguing exercise of the leg extensors. However, when collapsed across sex, wavelet-specific changes in spectral intensity over time reveal novel insights into the interplay between low- and high-frequency components during fatigue.