bluuebunny/crossref_metadata_2025 huggingface dataset:
A Review of Fatty Acid Biosynthesis Enzyme Inhibitors as Promising Antimicrobial Drugs
Authors: Bibens Laurie , Becker Jean-Paul , Dassonville-Klimpt Alexandra , Sonnet Pascal
Published: March 2023

Resistance to antimicrobial drugs is currently a serious threat to human health. Consequently, we are facing an urgent need for new antimicrobial drugs acting with original modes of action. The ubiquitous and widely conserved microbial fatty acid biosynthesis pathway, called FAS-II system, represents a potential target to tackle antimicrobial resistance. This pathway has been extensively studied, and eleven proteins have been described. FabI (or InhA, its homologue in mycobacteria) was considered as a prime target by many teams and is currently the only enzyme with commercial inhibitor drugs: triclosan and isoniazid. Furthermore, afabicin and CG400549, two promising compounds which also target FabI, are in clinical assays to treat Staphylococcus aureus. However, most of the other enzymes are still underexploited targets. This review, after presenting the FAS-II system and its enzymes in Escherichia coli, highlights the reported inhibitors of the system. Their biological activities, main interactions formed with their targets and structure–activity relationships are presented as far as possible.
Evaluation of ctDNA in the Prediction of Response to Neoadjuvant Therapy and Prognosis in Locally Advanced Rectal Cancer Patients: A Prospective Study
Authors: Morais Marina , Fonseca Telma , Melo-Pinto Diogo , Prieto Isabel , Vilares Ana Teresa , Duarte Ana Luísa , Leitão Patrícia , Cirnes Luís , Machado José Carlos , Carneiro Silvestre
Published: March 2023

“Watch and wait” is becoming a common treatment option for patients with locally advanced rectal cancer (LARC) submitted to neoadjuvant treatment. However, currently, no clinical modality has an acceptable accuracy for predicting pathological complete response (pCR). The aim of this study was to assess the clinical utility of circulating tumor DNA (ctDNA) in predicting the response and prognosis in these patients. We prospectively enrolled a cohort of three Iberian centers between January 2020 and December 2021 and performed an analysis on the association of ctDNA with the main response outcomes and disease-free survival (DFS). The rate of pCR in the total sample was 15.3%. A total of 24 plasma samples from 18 patients were analyzed by next-generation sequencing. At baseline, mutations were detected in 38.9%, with the most common being TP53 and KRAS. Combination of either positive magnetic resonance imaging (MRI) extramural venous invasion (mrEMVI) and ctDNA increased the risk of poor response (p = 0.021). Also, patients with two mutations vs. those with fewer than two mutations had a worse DFS (p = 0.005). Although these results should be read carefully due to sample size, this study suggests that baseline ctDNA combined with mrEMVI could potentially help to predict the response and baseline ctDNA number of mutations might allow the discrimination of groups with different DFS. Further studies are needed to clarify the role of ctDNA as an independent tool in the selection and management of LARC patients.
A Safe-by-Design Approach for the Synthesis of a Novel Cross-Linked Hyaluronic Acid with Improved Biological and Physical Properties
Authors: Sciabica Sabrina , Barbari Riccardo , Fontana Riccardo , Tafuro Giovanni , Semenzato Alessandra , Traini Daniela , Silva Dina M. , Reis Larissa Gomes Dos , Canilli Luisa , Terno Massimo , Marconi Peggy , Baldisserotto Anna , Vertuani Silvia , Manfredini Stefano
Published: March 2023

Hyaluronic acid (HA) is a polymer with unique biological properties that has gained in interest over the years, with applications in pharmaceutical, cosmetic, and biomedical fields; however, its widespread use has been limited by its short half-life. Therefore, a new cross-linked hyaluronic acid was designed and characterized using a natural and safe cross-linking agent, such as arginine methyl ester, which provided improved resistance to enzymatic action, as compared to the corresponding linear polymer. The antibacterial profile of the new derivative was shown to be effective against S. aureus and P. acnes, making it a promising candidate for use in cosmetic formulations and skin applications. Its effect on S. pneumoniae, combined with its excellent tolerability profile on lung cells, also makes this new product suitable for applications involving the respiratory tract.
Therapeutic Inhalation of Hydrogen Gas for Alzheimer’s Disease Patients and Subsequent Long-Term Follow-Up as a Disease-Modifying Treatment: An Open Label Pilot Study
Authors: Ono Hirohisa , Nishijima Yoji , Ohta Shigeo
Published: March 2023

(1) Background: Alzheimer’s disease (AD) is a progressive and fatal neurodegenerative disorder. Hydrogen gas (H2) is a therapeutic medical gas with multiple functions such as anti-oxidant, anti-inflammation, anti-cell death, and the stimulation of energy metabolism. To develop a disease-modifying treatment for AD through multifactorial mechanisms, an open label pilot study on H2 treatment was conducted. (2) Methods: Eight patients with AD inhaled 3% H2 gas for one hour twice daily for 6 months and then followed for 1 year without inhaling H2 gas. The patients were clinically assessed using the Alzheimer’s Disease Assessment Scale-cognitive subscale (ADAS-cog). To objectively assess the neuron integrity, diffusion tensor imaging (DTI) with advanced magnetic resonance imaging (MRI) was applied to neuron bundles passing through the hippocampus. (3) Results: The mean individual ADAS-cog change showed significant improvement after 6 months of H2 treatment (−4.1) vs. untreated patients (+2.6). As assessed by DTI, H2 treatment significantly improved the integrity of neurons passing through the hippocampus vs. the initial stage. The improvement by ADAS-cog and DTI assessments were maintained during the follow-up after 6 months (significantly) or 1 year (non-significantly). (4) Conclusions: This study suggests that H2 treatment not only relieves temporary symptoms, but also has disease-modifying effects, despite its limitations.
Emetic Tartar-Loaded Liposomes as a New Strategy for Leishmaniasis Treatment
Authors: Coelho Larissa D. , Souza Mirna M. D. , Cassali Geovanni D. , Silva Raphaela A. , Paiva Maria J. N. , Barros André L. B. , Teixeira Eliane M. , Silveira Josianne N. , Coelho Paulo M. Z. , Aguiar Marta M. G. , Oliveira Mônica C.
Published: March 2023

Emetic tartar (ET), was used in the treatment of leishmaniasis but its use was discontinued due to its low therapeutic index. Liposomes have been shown to be a promising strategy for delivery of bioactive substances in the region of interest, in order to reduce and/or eliminate undesirable effects. In the present study, liposomes containing ET were prepared and characterized to evaluate acute toxicity as well as their leishmanicidal action using BALB/c mice with an inoculum of Leishmania (Leishmania) infantum. Liposomes were composed of egg phosphatidylcholine and 3ß-[N-(N′,N′-dimethylaminoethane)-carbamoyl]cholesterol, with an average diameter of 200 nm, zeta potential of +18 mV, and ET encapsulated into liposomes at a concentration near 2 g/L. Healthy mice were treated with ET or liposome containing ET (Lip-ET) in a single dose of 16 mg/kg of Sb3+ intravenously and observed for 14 days. The death of two animals in the ET-treated group and no deaths in the Lip-ET-treated group was observed. Higher hepatic and cardiac toxicity were observed in animals treated with ET when compared to animals treated with Lip-ET, blank liposomes (Blank-Lip) and PBS. The study of antileishmanial efficacy was conducted by intraperitoneal administration of Lip-ET, for ten consecutive days. It was observed by limiting dilution that treatments with liposomal formulations containing ET, as well as Glucantime®, led to a significant reduction in parasitic load in spleen and liver (p < 0.05) when compared to the untreated control group.
Hospital Production of Sterile 2% Propofol Nanoemulsion: Proof of Concept
Authors: Cèbe Amélie , Dessane Bérangère , Gohier Pauline , Bernadou Jean-Marc , Venet Arnaud , Xuereb Fabien , Crauste-Manciet Sylvie
Published: March 2023

In the context of essential drug shortages, this article reports a proof of concept for the hospital preparation of a 2% propofol injectable nanoemulsion. Two processes for propofol were assessed: mixing propofol with the commercial Intralipid® 20% emulsion and a “de novo” process performed using separate raw materials (i.e., oil, water, and surfactant) and optimized for droplet size reduction with a high-pressure homogenizer. A propofol HPLC-UV stability-indicating method was developed for process validation and short-term stability. In addition, free propofol in the aqueous phase was quantified by dialysis. To envision routine production, sterility and endotoxin tests were validated. Only the “de novo” process using high-pressure homogenization gave satisfactory physical results similar to commercialized Diprivan® 2%. Both terminal heat sterilization processes (121 °C, 15 min and 0.22 µm filtration) were validated, but an additional pH adjustment was required prior to heat sterilization. The propofol nanoemulsion was monodisperse with a 160 nm mean droplet size, and no droplets were larger than 5µm. We confirmed that free propofol in the aqueous phase of the emulsion was similar to Diprivan 2%, and the chemical stability of propofol was validated. In conclusion, the proof of concept for the in-house 2% propofol nanoemulsion preparation was successfully demonstrated, opening the field for the possible production of the nanoemulsion in hospital pharmacies.
Quantitative Analysis of Photothermal Therapy of Tumor Tissue Using Various Gold Nanoparticle Injection Schemes
Authors: Kim Donghyuk , Kim Hyunjung
Published: March 2023

Photothermal therapy is a new chemotherapy technique using photothermal effects, a phenomenon in which light energy is converted into thermal energy. Since the treatment technique is performed without surgical incision, it does not cause bleeding and patients are expected to make rapid recoveries, which are significant advantages. In this study, photothermal therapy with direct injection of gold nanoparticles into tumor tissue was simulated through numerical modeling. The treatment effect resulting from changing the intensity of the irradiated laser, volume fraction of the injected gold nanoparticles, and number of gold nanoparticle injections was quantitatively evaluated. The discrete dipole approximation method was applied to calculate the optical properties of the entire medium, and the Monte Carlo method was applied to identify the absorption and scattering behavior of lasers in tissue. In addition, by confirming the temperature distribution of the entire medium through the calculated light absorption distribution, the treatment effect of photothermal therapy was evaluated, and the optimal treatment conditions were suggested. This is expected to accelerate the popularization of photothermal therapy in the future.
DOPE/CHEMS-Based EGFR-Targeted Immunoliposomes for Docetaxel Delivery: Formulation Development, Physicochemical Characterization and Biological Evaluation on Prostate Cancer Cells
Authors: Moreira Thais da Silva , Silva Alan Denis Olivindo , Vasconcelos Bianca Rodrigues Farias , Santos Elias da Silva , de Sousa Ana Carolina Cruz , de Freitas João Vito Barroso , de Oliveira Yara Santiago , Vidal Laura Maria Teodorio , Ribeiro Fábio de Oliveira Silva , de Araújo Alyne Rodrigues , Vieira Neto José de Brito , Pessoa Cláudia do Ó , Petrilli Raquel , Eloy Josimar O.
Published: March 2023

Docetaxel (DTX) is a non-selective antineoplastic agent with low solubility and a series of side effects. The technology of pH-sensitive and anti-epidermal growth factor receptor (anti-EGFR) immunoliposomes aims to increase the selective delivery of the drug in the acidic tumor environment to cells with EFGR overexpression. Thus, the study aimed to develop pH-sensitive liposomes based on DOPE (dioleoylphosphatidylethanolamine) and CHEMS (cholesteryl hemisuccinate), using a Box–Behnken factorial design. Furthermore, we aimed to conjugate the monoclonal antibody cetuximab onto liposomal surface, as well as to thoroughly characterize the nanosystems and evaluate them on prostate cancer cells. The liposomes prepared by hydration of the lipid film and optimized by the Box–Behnken factorial design showed a particle size of 107.2 ± 2.9 nm, a PDI of 0.213 ± 0.005, zeta potential of −21.9 ± 1.8 mV and an encapsulation efficiency of 88.65 ± 20.3%. Together, FTIR, DSC and DRX characterization demonstrated that the drug was properly encapsulated, with reduced drug crystallinity. Drug release was higher in acidic pH. The liposome conjugation with the anti-EGFR antibody cetuximab preserved the physicochemical characteristics and was successful. The liposome containing DTX reached an IC50 at a concentration of 65.74 nM in the PC3 cell line and 28.28 nM in the DU145 cell line. Immunoliposome, in turn, for PC3 cells reached an IC50 of 152.1 nM, and for the DU145 cell line, 12.60 nM, a considerable enhancement of cytotoxicity for the EGFR-positive cell line. Finally, the immunoliposome internalization was faster and greater than that of liposome in the DU145 cell line, with a higher EGFR overexpression. Thus, based on these results, it was possible to obtain a formulation with adequate characteristics of nanometric size, a high encapsulation of DTX and liposomes and particularly immunoliposomes containing DTX, which caused, as expected, a reduction in the viability of prostate cells, with high cellular internalization in EGFR overexpressing cells.
Immunomodulatory Activity of the Tyrosine Kinase Inhibitor Dasatinib to Elicit NK Cytotoxicity against Cancer, HIV Infection and Aging
Authors: Rodríguez-Agustín Andrea , Casanova Víctor , Grau-Expósito Judith , Sánchez-Palomino Sonsoles , Alcamí José , Climent Núria
Published: March 2023

Tyrosine kinase inhibitors (TKIs) have been extensively used as a treatment for chronic myeloid leukemia (CML). Dasatinib is a broad-spectrum TKI with off-target effects that give it an immunomodulatory capacity resulting in increased innate immune responses against cancerous cells and viral infected cells. Several studies reported that dasatinib expanded memory-like natural killer (NK) cells and γδ T cells that have been related with increased control of CML after treatment withdrawal. In the HIV infection setting, these innate cells are associated with virus control and protection, suggesting that dasatinib could have a potential role in improving both the CML and HIV outcomes. Moreover, dasatinib could also directly induce apoptosis of senescence cells, being a new potential senolytic drug. Here, we review in depth the current knowledge of virological and immunogenetic factors associated with the development of powerful cytotoxic responses associated with this drug. Besides, we will discuss the potential therapeutic role against CML, HIV infection and aging.
Biopolymer- and Lipid-Based Carriers for the Delivery of Plant-Based Ingredients
Authors: Gali Lynda , Pirozzi Annachiara , Donsì Francesco
Published: March 2023

Natural ingredients are gaining increasing attention from manufacturers following consumers’ concerns about the excessive use of synthetic ingredients. However, the use of natural extracts or molecules to achieve desirable qualities throughout the shelf life of foodstuff and, upon consumption, in the relevant biological environment is severely limited by their poor performance, especially with respect to solubility, stability against environmental conditions during product manufacturing, storage, and bioavailability upon consumption. Nanoencapsulation can be seen as an attractive approach with which to overcome these challenges. Among the different nanoencapsulation systems, lipids and biopolymer-based nanocarriers have emerged as the most effective ones because of their intrinsic low toxicity following their formulation with biocompatible and biodegradable materials. The present review aims to provide a survey of the recent advances in nanoscale carriers, formulated with biopolymers or lipids, for the encapsulation of natural compounds and plant extracts.
Water-Soluble Dicationic Deuteroporphyrin Derivative for Antimicrobial PDT: Singlet Oxygen Generation, Passive Carrier Interaction and Nosocomial Bacterial Strains Photoinactivation
Authors: Berezin Dmitry B. , Kruchin Sergey O. , Kukushkina Natal’ya V. , Venediktov Evgeny A. , Koifman Mikhail O. , Kustov Andrey V.
Published: March 2023

Multidrug resistance of pathogenic microflora is a serious threat to the modern community looking for new approaches to treating superinfections. In this sense, antimicrobial photodynamic therapy (aPDT) is an effective and safe technique considered to be a promising alternative or an important supplement to the traditional clinically applied methods for inactivating antibiotic resistant pathogens. Macroheterocyclic photosensitizers (PS) of three generations are proposed for clinical practice. They are known as the key compounds for PDT able to be localized selectively in microbial cells and to be activated with the red light producing toxic reactive oxygen species (ROS). However, these neutral and anionic PSs possess low affinity towards the outer lipopolysaccharide membrane of Gram-negative bacteria and, consequently, poor ability to kill these pathogens under irradiation. In contrast, cationic PSs containing one or more charged groups, especially those bound to an appropriate carrier, provide efficient inactivation of microorganisms. In this paper, we focus on the study of photophysics, aggregation and photoinduced antimicrobial activity of the water-soluble derivative of deuteroporphyrin-IX, a blood group porphyrin, bearing two cationic trialkylammonium fragments. This potential photosensitizing agent is found to generate singlet oxygen in a non-polar environment and forms stable nano-sized molecular complexes with passive non-ionic carrier Tween 80, localizing in an aqueous surfactant solution as a non-aggregated form in the surface micellar layer. Two different modes of PS/Tween 80 binding characterized by their own stability constants and interaction stoichiometry are observed. Microbiological experiments clearly demonstrate that the increased permeability of the outer bacterial membrane caused by the application of the intramicellar form of the photosensitizer or addition of some potentiation agents leads to pronounced light phototoxicity of the pigment against antibiotic-resistant nosocomial strains of Gram-negative bacterial pathogens.
OliveAtlas: A Gene Expression Atlas Tool for Olea europaea
Authors: Bullones Amanda , Castro Antonio Jesús , Lima-Cabello Elena , Alché Juan de Dios , Luque Francisco , Claros Manuel Gonzalo , Fernandez-Pozo Noe
Published: March 2023

The olive (Olea europaea L.) is an ancient crop of great importance in the Mediterranean basin due to the production of olive oil and table olives, which are important sources of fat and have benefits for human health. This crop is expanding and increasing its production worldwide and five olive genomes have recently been sequenced, representing a wild olive and important cultivars in terms of olive oil production, intensive agriculture, and adaptation to the East Asian climate. However, few bioinformatic and genomic resources are available to assist olive research and breeding, and there are no platforms to query olive gene expression data. Here, we present OliveAtlas, an interactive gene expression atlas for olive with multiple bioinformatics tools and visualization methods, enabling multiple gene comparison, replicate inspection, gene set enrichment, and data downloading. It contains 70 RNA-seq experiments, organized in 10 data sets representing the main olive plant organs, the pollen germination and pollen tube elongation process, and the response to a collection of biotic and abiotic stresses, among other experimental conditions. OliveAtlas is a web tool based on easyGDB with expression data based on the ‘Picual’ genome reference and gene annotation.
Comprehensive Analysis of BR Receptor Expression under Hormone Treatment in the Rubber Tree (Hevea brasiliensis Muell. Arg.)
Authors: Guo Bingbing , Dai Longjun , Yang Hong , Zhao Xizhu , Liu Mingyang , Wang Lifeng
Published: March 2023

Brassinosteroids (BRs) are important for plant growth and development, with BRI1 and BAK1 kinases playing an important role in BR signal transduction. Latex from rubber trees is crucial for industry, medicine and defense use. Therefore, it is beneficial to characterize and analyze HbBRI1 and HbBAK1 genes to improve the quality of the resources obtained from Hevea brasiliensis (rubber tree). Based on bioinformatics predictions and rubber tree database, five HbBRI1s with four HbBAK1s were identified and named HbBRI1~HbBRL3 and HbBAK1a~HbBAK1d, respectively, which were clustered in two groups. HbBRI1 genes, except for HbBRL3, exclusively contain introns, which is convenient for responding to external factors, whereas HbBAK1b/c/d contain 10 introns and 11 exons, and HbBAK1a contains eight introns. Multiple sequence analysis showed that HbBRI1s include typical domains of the BRI1 kinase, indicating that HbBRI1s belong to BRI1. HbBAK1s that possess LRR and STK_BAK1_like domains illustrate that HbBAK1s belong to the BAK1 kinase. BRI1 and BAK1 play an important role in regulating plant hormone signal transduction. Analysis of the cis-element of all HbBRI1 and HbBAK1 genes identified hormone response, light regulation and abiotic stress elements in the promoters of HbBRI1s and HbBAK1s. The results of tissue expression patterns indicate that HbBRL1/2/3/4 and HbBAK1a/b/c are highly expressed in the flower, especially HbBRL2-1. The expression of HbBRL3 is extremely high in the stem, and the expression of HbBAK1d is extremely high in the root. Expression profiles with different hormones show that HbBRI1 and HbBAK1 genes are extremely induced by different hormone stimulates. These results provide theoretical foundations for further research on the functions of BR receptors, especially in response to hormone signals in the rubber tree.
High-Nitrate-Supply-Induced Transcriptional Upregulation of Ascorbic Acid Biosynthetic and Recycling Pathways in Cucumber
Authors: Hesari Neda , Szegő Anita , Mirmazloum Iman , Pónya Zsolt , Kiss-Bába Erzsébet , Kolozs Henriett , Gyöngyik Márta , Vasas Dominika , Papp István
Published: March 2023

Nowadays open field and protected vegetable cultivation practices require and use genotypes which are precisely tailored to their intended growth environments. Variability of this kind provides a rich source of material to uncover molecular mechanisms supporting the necessarily divergent physiological traits. In this study, typical field-optimized and glasshouse-cultivated cucumber F1 hybrids were investigated, and displayed slower growth (‘Joker’) and faster growth (‘Oitol’) in seedlings. Antioxidant capacity was lower in ‘Joker’ and higher in ‘Oitol’, pointing to a potential redox regulation of growth. The growth response of seedlings to paraquat treatment indicated stronger oxidative stress tolerance in the fast-growing ‘Oitol’. To test whether protection against nitrate-induced oxidative stress was also different, fertigation with increasing potassium nitrate content was applied. This treatment did not change growth but decreased the antioxidant capacities of both hybrids. Bioluminescence emission revealed stronger lipid peroxidation triggered by high nitrate fertigation in the leaves of ‘Joker’ seedlings. To explore the background of the more effective antioxidant protection of ‘Oitol’, levels of ascorbic acid (AsA), as well as transcriptional regulation of relevant genes of the Smirnoff–Wheeler biosynthetic pathway and ascorbate recycling, were investigated. Genes related to AsA biosynthesis were strongly upregulated at an elevated nitrate supply in ‘Oitol’ leaves only, but this was only reflected in a small increase in total AsA content. High nitrate provision also triggered expression of ascorbate–glutathion cycle genes with stronger or exclusive induction in ‘Oitol’. AsA/dehydro–ascorbate ratios were higher in ‘Oitol’ for all treatments, with a more pronounced difference at high nitrate levels. Despite strong transcriptional upregulation of ascorbate peroxidase genes (APX) in ‘Oitol’, APX activity only increased significantly in ‘Joker’. This suggests potential inhibition of APX enzyme activity specifically in ‘Oitol’ at a high nitrate supply. Our results uncover an unexpected variability in redox stress management in cucumbers, including nitrate inducibility of AsA biosynthetic and recycling pathways in certain genotypes. Possible connections between AsA biosynthesis, recycling and nitro-oxidative stress protection are discussed. Cucumber hybrids emerge as an excellent model system for studying the regulation of AsA metabolism and the roles of AsA in growth and stress tolerance.
The Leaf Essential Oil of Myrtus communis subsp. tarentina (L.) Nyman: From Phytochemical Characterization to Cytotoxic and Antimigratory Activity in Human Prostate Cancer Cells
Authors: Giuliani Claudia , Moretti Roberta Manuela , Bottoni Martina , Santagostini Laura , Fico Gelsomina , Montagnani Marelli Marina
Published: March 2023

The aim of this study was to investigate the chemical profile and the cytotoxic activity in two castration-resistant prostate cancer (CRPC) cell lines of the leaf essential oil in Myrtus communis subsp. tarentina (L.) Nyman (EO MT), which was cultivated at the Ghirardi Botanical Garden (Toscolano Maderno, Brescia, Italy). The leaves were air-dried and extracted by hydrodistillation with a Clevenger-type apparatus, and the EO profile was characterized by GC/MS. For the cytotoxic activity investigation, we analyzed the cell viability by MTT assay, and the apoptosis induction by Annexin V/propidium iodide assay/Western blot analysis of cleaved caspase 3 and cleaved PARP proteins. Moreover, the cellular migration was analyzed by Boyden’s chamber assay and the distribution of actin cytoskeleton filaments by immunofluorescence. We identified 29 total compounds; the main compound classes were oxygenated monoterpenes, monoterpene hydrocarbons, and sesquiterpenes. The main constituents were α-pinene, α-humulene, α-terpineol, durohydroquinon, linalool, geranyl acetate, and β-caryophyllene. We found that EO MT was able to reduce cellular viability, activating an apoptotic process, and to decrease the migratory capacity of CRPC cells. These results suggest that it might be interesting to further investigate the effects of single compounds present in EO MT for their possible use in prostate cancer treatment.
Phytochemical-Based Nanomaterials against Antibiotic-Resistant Bacteria: An Updated Review
Authors: Díaz-Puertas Rocío , Álvarez-Martínez Francisco Javier , Falco Alberto , Barrajón-Catalán Enrique , Mallavia Ricardo
Published: March 2023

Antibiotic-resistant bacteria (ARB) is a growing global health threat, leading to the search for alternative strategies to combat bacterial infections. Phytochemicals, which are naturally occurring compounds found in plants, have shown potential as antimicrobial agents; however, therapy with these agents has certain limitations. The use of nanotechnology combined with antibacterial phytochemicals could help achieve greater antibacterial capacity against ARB by providing improved mechanical, physicochemical, biopharmaceutical, bioavailability, morphological or release properties. This review aims to provide an updated overview of the current state of research on the use of phytochemical-based nanomaterials for the treatment against ARB, with a special focus on polymeric nanofibers and nanoparticles. The review discusses the various types of phytochemicals that have been incorporated into different nanomaterials, the methods used to synthesize these materials, and the results of studies evaluating their antimicrobial activity. The challenges and limitations of using phytochemical-based nanomaterials, as well as future directions for research in this field, are also considered here. Overall, this review highlights the potential of phytochemical-based nanomaterials as a promising strategy for the treatment against ARB, but also stresses the need for further studies to fully understand their mechanisms of action and optimize their use in clinical settings.
Cyclodextrin-Based Polymeric Drug Delivery Systems for Cancer Therapy
Authors: Li Xuebing , Liu Junda , Qiu Neng
Published: March 2023

Cyclodextrins (CDs) are one of the most extensively studied cyclic-oligosaccharides due to their low toxicity, good biodegradability and biocompatibility, facile chemical modification, and unique inclusion capacity. However, problems such as poor pharmacokinetics, plasma membrane disruption, hemolytic effects and a lack of target specificity still exist for their applications as drug carriers. Recently, polymers have been introduced into CDs to combine the advantages of both biomaterials for the superior delivery of anticancer agents in cancer treatment. In this review, we summarize four types of CD-based polymeric carriers for the delivery of chemotherapeutics or gene agents for cancer therapy. These CD-based polymers were classified based on their structural properties. Most of the CD-based polymers were amphiphilic with the introduction of hydrophobic/hydrophilic segments and were able to form nanoassemblies. Anticancer drugs could be included in the cavity of CDs, encapsulated in the nanoparticles or conjugated on the CD-based polymers. In addition, the unique structures of CDs enable the functionalization of targeting agents and stimuli-responsive materials to realize the targeting and precise release of anticancer agents. In summary, CD-based polymers are attractive carriers for anticancer agents.
Experimental and Numerical Investigation on the Influence Factors of Damage Interference of Patch-Repaired CFRP Laminates under Double Impacts
Authors: Sun Zhenhui , Li Cheng , Tie Ying
Published: March 2023

The impact responses of a patch-repaired carbon-fiber-reinforced polymer (CFRP) specimen under double impacts were compared to study the damage interference mechanism through the combination of experiment and numerical analysis. A three-dimensional finite element model (FEM) with iterative loading based on continuous damage mechanics (CDM) and a cohesive zone model (CZM) was employed to simulate the double-impacts testing with an improved movable fixture at an impact distance of 0 mm–50 mm. The influence of impact distance and impact energy on the damage interference was explored by mechanical curves and delamination damage diagrams of the repaired laminates. When impactors fell within the range of the patch with an impact distance of 0 mm–25 mm at a low level of impact energy, delamination damage of the parent plate caused by the two impacts overlapped, resulting in damage interference. With the continuing increase in impact distance, the damage interference gradually disappeared. When impactors fell on the edge of the patch, the damage area caused by the first impact on the left half of the adhesive film gradually enlarged, and as the impact energy increased from 5 J to 12.5 J, the damage interference caused by the first impact on the second impact was gradually enhanced.
Epoxidized and Maleinized Hemp Oil to Develop Fully Bio-Based Epoxy Resin Based on Anhydride Hardeners
Authors: Lerma-Canto Alejandro , Samper Maria D. , Dominguez-Candela Ivan , Garcia-Garcia Daniel , Fombuena Vicent
Published: March 2023

The present work aims to develop thermosetting resins using epoxidized hemp oil (EHO) as a bio-based epoxy matrix and a mixture of methyl nadic anhydride (MNA) and maleinized hemp oil (MHO) in different ratios as hardeners. The results show that the mixture with only MNA as a hardener is characterized by high stiffness and brittleness. In addition, this material is characterized by a high curing time of around 170 min. On the other hand, as the MHO content in the resin increases, the mechanical strength properties decrease and the ductile properties increase. Therefore, it can be stated that the presence of MHO confers flexible properties to the mixtures. In this case, it was determined that the thermosetting resin with balanced properties and high bio-based content contains 25% MHO and 75% MNA. Specifically, this mixture obtained a 180% higher impact energy absorption and a 195% lower Young’s modulus than the sample with 100% MNA. Also, it has been observed that this mixture has significantly shorter times than the mixture containing 100% MNA (around 78 min), which is of great concern at an industrial level. Therefore, thermosetting resins with different mechanical and thermal properties can be obtained by varying the MHO and MNA content.
Photo-Durable Molecularly Oriented Liquid Crystalline Copolymer Film based on Photoalignment of N-benzylideneaniline
Authors: Nakajima Gento , Kondo Mizuho , Sakamoto Moritsugu , Sasaki Tomoyuki , Ono Hiroshi , Kawatsuki Nobuhiro
Published: March 2023

Copolymer films of photoalignable liquid crystalline (LC) copolymethacrylates comprised of a phenyl benzoate mesogen connected with N-benzylideneaniline end moiety (NBA2) and benzoic acid (BA) side groups exhibit a photoinduced reorientation behavior. Significant thermally stimulated molecular reorientation attains a dichroism (D) greater than 0.7 for all copolymer films and a birefringence of 0.113–0.181. In situ thermal hydrolysis of the oriented NBA2 groups decreases the birefringence to 0.111–0.128. However, the oriented structures of the film are maintained, demonstrating a photo-durability, even though the NBA2 side groups photo-react. The hydrolyzed oriented films show higher photo-durability without changing their optical properties.
Epoxy Resins for Flooring Applications, an Optimal Host for Recycling Deactivated Cement Asbestos
Authors: Campanale Fabrizio , Vergani Fabrizio , Marian Narcisa Mihaela , Viti Cecilia , Bianchi Alberto , Ferrario Silvia , Mauri Michele , Capitani Giancarlo
Published: March 2023

Cement asbestos slates, commonly known as Eternit® and still abundant in private and public buildings, were deactivated through a thermal process. The resulting deactivated cement asbestos powder (DCAP), a mixture of Ca-Mg-Al silicates and glass, was compounded with Pavatekno Gold 200 (PT) and Pavafloor H200/E (PF), two different epoxy resins (bisphenol A epichlorohydrin) for flooring applications. The addition of the DCAP filler to the PF samples causes a slight but acceptable decrease in the relevant mechanical properties (compressive, tensile, and flexural strengths) upon increasing DCAP content. The addition of the DCAP filler to pure epoxy (PT resin) causes a slight decrease in the tensile and flexural strengths with increasing DCAP content, while the compressive strength is almost unaffected, and the Shore hardness increases. The main mechanical properties of the PT samples are significantly better than those of the filler-bearing sample of normal production. Overall, these results suggest that DCAP can be advantageously used as filler in addition to, or in substitution for, commercial barite. In particular, the sample with 20 wt% of DCAP is the best performing in terms of compressive, tensile, and flexural strengths, whereas the sample with 30 wt% of DCAP shows the highest Shore hardness, which is an important property to be considered in flooring applications.
Exclusive Biosynthesis of Pullulan Using Taguchi’s Approach and Decision Tree Learning Algorithm by a Novel Endophytic Aureobasidium pullulans Strain
Authors: Saber WesamEldin I. A. , Al-Askar Abdulaziz A. , Ghoneem Khalid M.
Published: March 2023

Pullulan is a biodegradable, renewable, and environmentally friendly hydrogel biopolymer, with potential uses in food, medicine, and cosmetics. New endophytic Aureobasidium pullulans (accession number; OP924554) was used for the biosynthesis of pullulan. Innovatively, the fermentation process was optimized using both Taguchi’s approach and the decision tree learning algorithm for the determination of important variables for pullulan biosynthesis. The relative importance of the seven tested variables that were obtained by Taguchi and the decision tree model was accurate and followed each other’s, confirming the accuracy of the experimental design. The decision tree model was more economical by reducing the quantity of medium sucrose content by 33% without a negative reduction in the biosynthesis of pullulan. The optimum nutritional conditions (g/L) were sucrose (60 or 40), K2HPO4 (6.0), NaCl (1.5), MgSO4 (0.3), and yeast extract (1.0) at pH 5.5, and short incubation time (48 h), yielding 7.23% pullulan. The spectroscopic characterization (FT-IR and 1H-NMR spectroscopy) confirmed the structure of the obtained pullulan. This is the first report on using Taguchi and the decision tree for pullulan production by a new endophyte. Further research is encouraged for additional studies on using artificial intelligence to maximize fermentation conditions.
Effect of Lanthanum Sorption on the Behavior of Rarely Crosslinked Acidic and Basic Polymer Hydrogels during Remote Interaction
Authors: Melnikov Yevgeniy , Kondaurov Ruslan , Agibayeva Laura
Published: March 2023

This present study is targeted at the complex investigation of the behavior of interpolymer systems based on acidic rarely crosslinked polymeric hydrogels (polyacrylic acid hydrogel (hPAA); polymethacrylic acid hydrogel (hPMAA)) and basic rarely crosslinked polymeric hydrogels (poly-4-vinylpyridine hydrogel (hP4VP), specifically the poly-2-methyl-5-vinylpyridine hydrogel (hP2M5VP)) either in an aqueous medium or lanthanum nitrate solution. We found that the transition of the polymeric hydrogels in the developed interpolymer systems (hPAA-hP4VP, hPMAA-hP4VP, hPAA-hP2M5VP, and hPMAA-hP2M5VP) into highly ionized states leads to significant changes in electrochemical, conformational, and sorption properties of the initial macromolecules. The subsequent mutual activation effect demonstrates strong swelling of both hydrogels in the systems. The sorption efficiency of lanthanum by the interpolymer systems is 94.51% (33%hPAA:67%hP4VP), 90.80% (17%hPMAA-83%hP4VP), 91.55% (67%hPAA:33%hP2M5VP), and 90.10% (50%hPMAA:50%hP2M5VP). An advantage of the interpolymer systems (compared to individual polymeric hydrogels) is the strong growth of their sorption properties (up to 35%) due to high ionization states. Interpolymer systems can be considered new-generation sorbents for further application in the industry for highly effective sorption of rare earth metals.
Synthesis of Chemically Modified Acid-Functionalized Multiwall Carbon Nanotubes with Benzimidazole for Removal of Lead and Cadmium Ions from Wastewater
Authors: Elghamry Ibrahim , Gouda Mohamed , Al-Fayiz Yasair S. S.
Published: March 2023

In this work, acid-functionalized multiwalled carbon (MWCNTs–CO2H) nanotube was successfully functionalized with a heterocyclic scaffold, namely benzimidazole, to give novel functionalized multiwalled carbon nanotubes (BI@MWCNTs). Then, FTIR, XRD, TEM, EDX, Raman spectroscopy, DLS, and BET analyses were used to characterize the synthesized BI@MWCNTs. The effectiveness of the adsorption of two heavy metal ions, Cd2+ and Pb2+, in single metal and mixed metal solutions on the prepared material was investigated. Influencing parameters for the adsorption method, for example duration, pH, starting metal concentration, and BI@MWCNT dosage, were examined for both metal ions. Moreover, adsorption equilibrium isotherms fit with the Langmuir and Freundlich models perfectly, while the intra-particle diffusion models provide pseudo-second order adsorption kinetics. The adsorption of Cd2+ and Pb2+ ions onto BI@MWCNTs revealed an endothermic and a spontaneous method with great affinity as a result of the negative values of Gibbs free energy (ΔG) and the positive values of enthalpy (ΔH) and entropy (ΔS). Both Pb2+ and Cd2+ ions were completely eliminated from aqueous solution (100 and 98%, respectively) using the prepared material. Additionally, BI@MWCNTs have a high adsorption capacity and were regenerated in a simple way and reused for six cycles, which make them a cost-effective and efficient absorbent for the removal of such heavy metal ions from wastewater.
How to Protect One’s Home in Medieval China? A Study of the Fóshuō ānzhái shénzhòu jīng 佛說安宅神呪經
Authors: Yang Gang , Anderl Christoph
Published: March 2023

The protection of one’s home and ensuring the safety of one’s family have been deep-rooted concerns throughout time and in all cultures. Ānzhái 安宅 (“pacifying one’s residence”) rituals can be traced to ancient China and are still practiced in contemporary China. In this study, we will focus on the Fóshuō ānzhái shénzhòu jīng 佛說安宅神呪經 (AZSZJ; T.21, No. 1394), one of the extant Buddhist scriptures dealing with home protection. There have been (at least) two lines of transmission of ānzhái scriptures and on the basis of internal and external evidence, we project the compilation of the extant version of this text to include the late 6th century and mid 7th century and show that the scripture—in earlier catalogues labeled as “fake”—entered the Buddhist canon on the basis of a mistake or confusion by Míng Quán when he recorded it in his Dà-Zhōu kāndìng zhòngjīng mùlù大周刊定眾經目錄. The main part of the study consists of an annotated translation of AZSZJ and a preliminary analysis of the difficult terminology appearing in parts of the text. In the last part, we discuss some aspects of the text’s traditional Chinese and Buddhist elements and how they were skillfully combined in order to make the overall text attractive for the medieval Chinese Buddhist community and to successfully compete with other contemporary ritual practices concerned with the safety of one’s home.
Attributions of LGBTQ+ Identity and Religious Views on Homosexuality to Disaffiliation from Orthodox Judaism
Authors: Vulakh Gennady , Miles Rona , Chavarga Alla , Hirsch Estee , Eisen Pesach
Published: March 2023

Religious disaffiliation has been found to occur at higher rates in LGBTQ+ individuals than in heterosexual individuals and in the general population. In this brief report, we explore whether LGBTQ+ people who disaffiliated from Orthodox Judaism attributed sexual identity and/or Orthodox Judaism’s lack of acceptance of homosexuality to their disaffiliation. This analysis focuses on 117 individuals who identified as LGBTQ+ who were drawn from a larger study that included 387 participants across all sexual orientations who disaffiliated from Orthodox Judaism. Unexpectedly, only 18 of the respondents reported that their sexual identity and/or religious views on homosexuality were causes for their disaffiliation. A lack of education and language around LGBTQ+ concepts in ultra-Orthodox Jewish communities may have contributed to this finding. We suggest that additional research be conducted to explore the complex relationship between LGBTQ+ identity and disaffiliation from Orthodox Judaism.
A Multisensor UAV Payload and Processing Pipeline for Generating Multispectral Point Clouds
Authors: Vlaminck Michiel , Diels Laurens , Philips Wilfried , Maes Wouter , Heim René , Wit Bart De , Luong Hiep
Published: March 2023

Over the last two decades, UAVs have become an indispensable acquisition platform in the remote sensing community. Meanwhile, advanced lightweight sensors have been introduced in the market, including LiDAR scanners with multiple beams and hyperspectral cameras measuring reflectance using many different narrow-banded filters. To date, however, few fully fledged drone systems exist that combine different sensing modalities in a way that complements the strengths and weaknesses of each. In this paper, we present our multimodal drone payload and sensor fusion pipeline, which allows multispectral point clouds to be generated at subcentimeter accuracy. To that end, we combine high-frequency navigation outputs from a professional-grade GNSS with photogrammetric bundle adjustment and a dedicated point cloud registration algorithm that takes full advantage of LiDAR’s specifications. We demonstrate that the latter significantly improves the quality of the reconstructed point cloud in terms of fewer ghosting effects and less noise. Finally, we thoroughly discuss the impact of the quality of the GNSS/INS system on the structure from the motion and LiDAR SLAM reconstruction process.
Correction: Hua et al. Path Planning of Spacecraft Cluster Orbit Reconstruction Based on ALPIO. Remote Sens. 2022, 14, 4768
Authors: Hua Bing , Yang Guang , Wu Yunhua , Chen Zhiming
Published: March 2023

In the original article [...]
Evaluation and Analysis of Remotely Sensed Water Vapor from the NASA VIIRS/SNPP Product in Mainland China Using GPS Data
Authors: Zhou Linghao , Fan Lei , Shi Chuang
Published: March 2023

Precipitable water vapor (PWV) is a vitally important factor in atmospheric circulation. PWV is significant for forecasting extreme weather and understanding the dynamics of climate change. Comprehensively evaluating the performance of newly proposed remotely sensed water vapor products is crucial for guaranteeing their suitability for futural PWV applications. In this study, the accuracy of the recently established remotely sensed water vapor product from the Visible Infrared Imaging Radiometer Suite (VIIRS) satellite sensor on the Suomi National Polar-orbiting Partnership (SNPP) (VIIRS-PWV) platform within various regions of mainland China was evaluated via the PWV from Global Positioning System (GPS) observations. The GPS-derived PWV from 231 stations of the Crustal Movement Observation Network of China (CMONOC) from 2012 to 2018 was obtained through precise point positioning (PPP) techniques. The results showed that the mean value of the correlation coefficient (CC), the mean bias (MB), and the root-mean-square error (RMSE) between the VIIRS-PWV and the GPS-PWV were 0.92, −1.6 mm, and 4.7 mm, respectively. These values were comparable with the results of the PWV data derived from the Atmospheric Infrared Sounder (AIRS) and Moderate Resolution Imaging Spectroradiometer (MODIS) products. This indicates that the VIIRS product could provide PWV data with satisfactory accuracy for large-area scientific applications. Moreover, the MB and RMSE of the differences between the GPS-PWV and VIIRS-PWV showed obvious seasonal variations. The VIIRS-PWV generally performed better in winter (with the MB and RMSE values of 0.1 mm and 2.3 mm) than in summer (with the MB and RMSE values of −4.4 and 7.0 mm). Analysis among different regions revealed that the Central South (CS) region of China attained the largest mean RMSE value of 6.3 mm, and the North West (NW) region attained the smallest mean RMSE value of 3.8 mm. In addition, the southern region of China obtained a mean RMSE value of 5.6 mm, while that for the northern region of China was 3.9 mm. This indicates that the VIIRS-PWV has better accuracy within the northern region of China than within the southern region.