bluuebunny/crossref_metadata_2025 huggingface dataset:
Radiation-Induced Hemorrhagic Cystitis in Prostate Cancer Survivors: The Hidden Toll
Authors: Gatsinga René , Lim Benjamin J. H. , Kumar Navin , Tan Jacinda G. G. , Li Youquan , Wang Michael L. C. , Tan Terence W. K. , Tuan Jeffrey K. L. , Tan Yu Guang , Chen Kenneth , Yuen John S. P.
Published: October 2024

Background and Objectives: Radiation therapy (RT) plays a crucial role in managing prostate cancer, offering effective disease control and improving survival rates in both localized and recurrent cases. However, RT can lead to hemorrhagic cystitis, a significant late complication resulting in chronic morbidity and other health issues. This study aims to evaluate the real-world incidence of radiation-induced hemorrhagic cystitis requiring surgical intervention. Materials and Methods: This retrospective cohort study analyzed data from prostate cancer survivors treated for hematuria at our center between January 2014 and January 2024. Patients were included if cystoscopy identified radiation cystitis as the cause of hematuria. Descriptive statistics were used, and binomial logistic regression analyses with univariate and multivariate analysis were performed to identify risk factors for worse outcomes. Results: Fifty-two patients met the inclusion criteria. The estimated cumulative incidence at a median follow-up of 5.3 years was 4.5%. Among the participants, 21.2% required more than two transurethral bladder fulguration (TUBF) procedures, and 38.5% needed more than two hospital admissions for hematuria management. The median time to the first fulguration was 64 months. Blood transfusions were necessary in 53.8% of cases, and 38.5% required hyperbaric oxygen therapy. Ultimately, 5.8% of the patients underwent cystectomy. Univariate analysis identified ischemic heart disease (IHD) and antiplatelet therapy as significant risk factors (OR: 5.17 and 5.18, respectively), along with longer time to first fulguration (OR: 5.02). Multivariate analysis confirmed antiplatelet therapy (OR: 2.8, p = 0.05) and time to first TUBF (OR: 1.8, p = 0.02) as significant predictors of multiple procedures. Conclusions: Radiation cystitis remains a significant burden on prostate cancer survivors. Patients on antithrombotic agents, those with delayed initial presentations, and those who received radiation as salvage therapy are more likely to experience higher morbidity.
The Jack-in-the-Box: Pericardial Decompression Syndrome Managed by a Multidisciplinary Approach with Early Initiation of Veno-Arterial Extracorporeal Membrane Oxygenation: A Case Report
Authors: Orban Carmen , Borjog Tudor , Talpau Claudia , Agapie Mihaela , Bratu Angelica , Jafal Mugurel , Popescu Mihai
Published: October 2024

Post decompression syndrome (PDS) is a rare and life-threatening complication of pericardiocentesis, especially after rapid drainage of large amounts of pericardial fluid. We present the case of a 21-year-old man who presented with cardiac tamponade of unknown etiology. After preoperative optimization, surgical drainage of the pericardial effusion was performed and approximately 2500 mL of fluid was released over 30 min. The patient rapidly developed hemodynamic collapse with severe biventricular dysfunction, with a left ventricle ejection fraction of 15%. Vasopressor and inotropic support were initiated with Noradrenaline and Dobutamine, further escalated to Adrenaline and Levosimendan with no improvement in clinical and hemodynamic parameters. Considering the high doses of vasoactive drugs, rescue veno-arterial extracorporeal membrane oxygenation (V-A ECMO) was started within the first 24 h. After 10 days on V-A ECMO, the cardiac function slowly recovered, and the extracorporeal mechanical support was successfully weaned. The diagnosis of paraneoplastic PDS secondary to angiosarcoma was made and the patient was successfully discharged to the ward on the 24th day. In conclusion, far from being the last option in the management of PDS, V-A ECMO deserves early consideration for securing adequate myocardial and systemic perfusion, while the cardiac function recovers, but a risk-to-benefit assessment should be made by an experienced multidisciplinary team.
Elevated Urotensin-II and TGF-β Levels in COPD: Biomarkers of Fibrosis and Airway Remodeling in Smokers
Authors: Kilinc Metin , Demir Ibrahim , Aydemir Semih , Gul Rauf , Dokuyucu Recep
Published: October 2024

Background and Objectives: Small airway fibrosis plays a critical role in the progression of chronic obstructive pulmonary disease (COPD). Previous research has suggested that Urotensin-II (U-II) and transforming growth factor-β (TGF-β) may contribute to pathological fibrosis in various organs, including the cardiovascular system, lungs, and liver. However, their specific relationship with airway fibrosis in COPD has not yet been thoroughly investigated. This study aims to evaluate the concentrations of U-II and TGF-β in individuals with COPD, as well as in healthy smokers and non-smokers, to explore their potential roles in COPD-related fibrosis. Materials and Methods: The study included three distinct groups: a healthy non-smoker control group (n = 98), a healthy smoker group (n = 78), and a COPD group (n = 80). All participants in the COPD group had a smoking history of at least 10 pack-years. COPD was defined according to the Global Initiative for Chronic Obstructive Lung Disease (GOLD) guidelines, with only patients classified as GOLD stage 2 or higher being included in the study. Urotensin-II (U-II) and transforming growth factor-β (TGF-β) levels were measured using a commercially available ELISA kit. Results: COPD patients had a significantly lower FEV1 (58 ± 15.4%) compared to smokers (79 ± 4.5%) and non-smokers (92 ± 3.7%) (p < 0.001). Similarly, COPD patients had a lower FEV1/FVC ratio (55 ± 9.4%) compared to smokers (72 ± 4.2%) and non-smokers (85 ± 3.6%) (p < 0.01 and p < 0.05, respectively). SaO2 was significantly lower in COPD patients (87%) compared to smokers (96.5%) and non-smokers (98%) (COPD vs. smokers: p < 0.05 and smokers vs. non-smokers: p > 0.05). U-II levels were significantly higher in COPD patients (175.10 ± 62.40 pg/mL) compared to smokers (118.50 ± 45.51 pg/mL) and non-smokers (85.29 ± 35.87 pg/mL) (p < 0.001 and p < 0.05, respectively). COPD patients also had significantly higher levels of TGF-β (284.60 ± 60.50 pg/mL) compared to smokers (160.00 ± 41.80 pg/mL) and non-smokers (92.00 ± 25.00 pg/mL) (p < 0.001 and p < 0.05, respectively). Conclusions: Our study supports the growing body of evidence that U-II and TGF-β play central roles in the development and progression of fibrosis in COPD. The negative correlation between these markers and lung function parameters such as FEV1 and FEV1/FVC indicates that they may be key drivers of airway remodeling and obstruction. These biomarkers could serve as early indicators of fibrotic changes in smokers, even before the onset of COPD.
The Importance of Biochemical Parameters, Immunonutritional Status, and Social Support for Quality of Life in Chronic Hemodialysis Patients
Authors: Babovic Batric , Belada Babovic Natasa , Tomovic Filip , Radovanovic Snezana , Debeljevic Mladen , Mustur Dusan , Mihaljevic Olgica
Published: October 2024

Background and Objectives: Chronic kidney disease (CKD) is a growing public health problem and one of the leading causes of premature death worldwide. The progressive nature of CKD is associated with serious complications that can reduce the quality of life in CKD patients. Additional factors that can worsen well-being include dialysis treatment, malnutrition, inflammation, and lack of social support. The aim of our study was to analyze the quality of life of CKD patients undergoing hemodialysis and its association with certain biochemical and immunonutritional parameters, as well as with social support. Materials and Methods: This research was conducted as a cross-sectional study that included 170 patients, divided into two groups: a group of patients undergoing hemodialysis (HD group) (n = 85), and a control group of non-hemodialysis patients (group with CKD stage 3–4) (n = 85). The Health-Related Quality of Life (HRQoL) score was used to assess the quality of life of the study population. Measurement of biochemical and immunonutritional parameters was also performed in all patients. The Oslo-3 Social Support Scale (OSSS-3) was used to analyze social support. Results: The HRQoL score was significantly lower in HD patients compared to patients with CKD stage 3–4 (0.701 ± 0.137 vs. 0.832 ± 0.122, p < 0.001). It declined significantly as the concentrations of urea (β = −0.347, p < 0.001), creatinine (β = −0.699, p = 0.005), uric acid (β = −0.184, p = 0.016), β2-microglobulin (β = −0.432, p < 0.001), and parathormone (β = −0.209, p = 0.006) increased in HD patients. In addition to uremic toxins, an increase in glucose (β = −0.278, p = 0.010) and triglyceride (β = −0.354, p = 0.001) concentrations was associated with poor HRQoL in patients with CKD stage 3–4. There was a significant connection between the Hemoglobin, Albumin, Lymphocyte, and Platelet (HALP) score and HRQoL in HD patients (β = 0.229, p = 0.035). Additionally, C-reactive protein (β = −0.361, p < 0.001) and neutrophil-to-lymphocyte ratio (β = −0.288, p < 0.001), as markers of systemic inflammation, directly affected HRQoL in HD patients. In both study groups, perceived social support positively influenced the HRQoL scores (β = 0.192, p = 0.012 for hemodialysis; β = 0.225, p = 0.038 for non-hemodialysis). Conclusions: There is a decline in HRQoL in chronic hemodialysis patients, significantly affected by certain biochemical and immunonutritional parameters, along with perceived social support.
Comparing NIRS and Pulse Oximetry for Cerebral Oxygen Saturation During Hypoxia Testing
Authors: Alevizakos Vasilios , Werner Andreas , Schiller Lisa-Marie , von See Constantin , Schiller Marcus
Published: October 2024

Objective: This study evaluates the suitability of cerebral oximetry using near-infrared spectroscopy (NIRS) compared to traditional pulse oximetry (SpO2) for measuring cerebral oxygen saturation during hypoxia testing, aiming to enhance safety during flight operations and training. Material and Methods: The study included 106 participants aged 18–60 years at the Aerospace Medicine Training Center in Königsbrück. Cerebral oxygen saturation (rSO2) and peripheral oxygen saturation (SpO2) were measured using the INVOS™ 5100C cerebral oximeter and Masimo™ MS5 pulse oximeter, respectively. Measurements were taken at baseline, during hypoxia at 25,000 feet, and post recovery. Data analysis included regression analysis, Bland–Altman plots, and concordance correlation coefficients (CCC). Ethical approval was obtained from the Hannover Medical School. Data from 100 participants were analyzed. Results: Baseline SpO2 was 97.5 ± 1.5%, and baseline rSO2 was 77.25 ± 6.4%. During hypoxia, SpO2 dropped significantly, while rSO2 showed higher values. SpO2 recovered faster than rSO2. Deviations in rSO2 between the right and left sides during hypoxia were minimal. Lin’s CCC indicated moderate to substantial concordance. NIRS measurements were more stable and less prone to disturbances, with 95 disruptions in pulse oximetry, 25 of which were potentially critical. Conclusions: NIRS is a reliable method for detecting cerebral oxygen saturation, offering significant advantages over traditional pulse oximetry in stability and reliability during hypoxia testing. NIRS is less error-prone, supporting its use for continuous monitoring in aviation settings and enhancing flight safety by providing more accurate hypoxia detection.
Empowering Leadership in the Military: Pros and Cons
Authors: Teixeira João , Pais Leonor , dos Santos Nuno Rebelo , de Sousa Bruno
Published: October 2024

The military serves as a vital bridge between strategic goals and societal values. This study aims to comprehensively analyze the potential and limitations of Empowering Leadership (EL) in the armed forces. Considering the current post-pandemic context and the geopolitical shifts in a volatile, uncertain, fragile, and complex world, we look at how EL can boost adaptability. We started by addressing military organization structure and evolution, followed by the EL concept and its impact on organizational performance. We discussed its benefits, progress, and role in modern military environments. The analysis points out EL benefits, despite the challenges posed by the military’s strict hierarchy, which can limit EL’s effectiveness, but concluded that this style can be effective when aligned with Mission Command. We analyze EL’s nuances in military settings, presenting three propositions and stating their potential, challenges, and limitations. This study offers insights into leadership dynamics in the military, highlighting the delicate balance between empowerment and traditional structures.
Enhancement of Activated Carbon on Anaerobic Fermentation of Heavy-Metal-Contaminated Plants: Insights into Microbial Responses
Authors: Tian Yonglan , Zhang Huayong , Zheng Lei , Cao Yudong , Tian Wang
Published: October 2024

Anaerobic fermentation is a potentially cost-effective approach to disposing of metal-contaminated biowaste collected during phytoremediation. However, the compound heavy metals contained in the biowaste may limit the efficiency of anaerobic fermentation. In this study, anaerobic fermentation with alfalfa harvested from an iron tailing as the feedstock was set up and further enhanced by granular activated carbon (AC). The results showed that adding AC improved the cumulative biogas yields of alfalfa contaminated with metals (AM) by 2.26 times. At the biogas peak stage, plenty of microbes were observed on the surface of the AC, and the functional groups of AC contributed to better electron transfer, lower heavy metal toxicity and higher CH4 contents. AC increased the richness and decreased the diversity of bacteria while reducing both the richness and diversity of archaea. The AC addition resulted in higher relative abundance of Prevotella_7, Bacteroides and Ruminiclostridium_1, which enhanced the hydrolysis of substrate and produced more precursors for methanogenesis. Meanwhile, the relative abundances of Methanosarcina and Methanobacterium were remarkably increased together with the metabolism of cofactors and vitamins, indicating the enhancement of both the acetoclastic and hydrotrophic methanogenesis. The present study provided new insights into the microbial responses of the anaerobic fermentation in heavy-metal-contaminated plants and proved the possibility of enhancing the biogas production by AC.
Optimization of Fermentation and Biocontrol Efficacy of Bacillus atrophaeus XHG-1-3m2
Authors: Xu Ziyan , Lu Hailong , Shi Wanbin , Zhou Xinmei , Ren Jianxin , Zhang Yanling , Ma Rong
Published: October 2024

Biological control plays an increasingly important role in various aspects of modern agriculture and forestry. Identifying biocontrol strains with commercial potential for effective disease management is currently a focal point in biological control research. In this study, Bacillus atrophaeus XHG-1-3m2, a strain with significant biocontrol potential against Wilsonomyces carpophilus causing shot hole disease in wild apricots, was developed. The study determined the antibacterial activity of the fermentation broth, the optimal fermentation medium composition and conditions, and explored its effectiveness in controlling Wilsonomyces carpophilus. The optimal fermentation medium for strain XHG-1-3m2 comprises 12.5 g/L yeast extract, 12.5 g/L soy peptone, 10.0 g/L sodium chloride, 1 g/L ammonium chloride, 1 g/L potassium dihydrogen phosphate, 1 g/L disodium hydrogen phosphate, and 0.5 g/L magnesium sulfate heptahydrate. With an initial pH of 7.0, a liquid volume of 40%, an inoculum volume of 3%, and shaking incubation at 28 °C for 24 h, the viable cell count reached 14 × 109 CFU/mL. In vitro and in vivo tests on leaves revealed that the fermentation broth and the biocontrol biofertilizer derived from this strain inhibited the leaf lesions caused by Wilsonomyces carpophilus on wild apricots, achieving inhibition rates of 94.62% and 82.46%, respectively.
Reactions of an Anionic Gallylene with Azobenzene or Azide Compounds Through C(sp2)–H and C(sp3)–H Activation
Authors: Sun Jinfeng , Chen Fangfeng , Liu Juan , Zhang Yihu , He Dongyu , Dodonov Vladimir A. , Zhao Yanxia
Published: October 2024

The activation of inert C–H bonds remains a challenge in current chemistry. Here, we report the excellent reactivity of the anionic gallylene species [LGa:][Na(THF)3] (L = [(2,6-iPr2C6H3)NC(CH3)]22−, 1) that allows the selective activation one ortho sp2 C–H bond of several azobenzene and azide derivatives at ambient temperature, with the transfer of the hydrogen atom to one of the nitrogen atoms. The process leads to the formation of the aryl amido products [LGa-κ2N,C-PhNN(H)(p-R-C6H3)][Na(solvent)3] (2, R = H solvent = DME (1,2-Dimethoxyethane); 3, R = –OMe, solvent = DME; 4, R = –NMe2 solvent = THF), [LGa-κ2N,C-(m-CH3-C6H4)NN(H)(m-CH3-C6H3)][Na(15-C-5)2] (5) with new Ga–C and Ga–N bonds. Moreover, 1 is also effective for the C–H activation of two azides RN3 (R = 2,4,6-Me3C6H2 or 2,6-iPr2C6H3), resulting in the formation of gallium amides [LGa(NH-2-(CH2)-4,6-Me2C6H2)][Na(15-C-5)2] (6) and [LGa(NH-2,6-iPr2C6H3)2][Na(THF)5] (7) through intra- or intermolecular sp3 C–H amination. Significantly, these reactions occur for the highly challenging activation of inert C(sp2)–H and C(sp3)–H bonds, thus demonstrating the excellent reactivity of the Ga(I) species 1. The products 2–7 were characterized by X-ray crystallography, 1H and 13C NMR, UV–vis spectroscopy, and density functional theory (DFT) calculations.
Mobility Support with Intelligent Obstacle Detection for Enhanced Safety
Authors: Han Jong Hyeok , Yoon Inkwon , Kim Hyun Soo , Jeong Ye Bin , Maeng Ji Hwan , Park Jinseok , Jeon Hee-Jae
Published: October 2024

In recent years, assistive technology usage among the visually impaired has risen significantly worldwide. While traditional aids like guide dogs and white canes have limitations, recent innovations like RFID-based indoor navigation systems and alternative sensory solutions show promise. Nevertheless, there is a need for a user-friendly, comprehensive system to address spatial orientation challenges for the visually impaired. This research addresses the significance of developing a deep learning-based walking assistance device for visually impaired individuals to enhance their safety during mobility. The proposed system utilizes real-time ultrasonic sensors attached to a cane to detect obstacles, thus reducing collision risks. It further offers real-time recognition and analysis of diverse obstacles, providing immediate feedback to the user. A camera distinguishes obstacle types and conveys relevant information through voice assistance. The system’s efficacy was confirmed with a 90–98% object recognition rate in tests involving various obstacles. This research holds importance in providing safe mobility, promoting independence, leveraging modern technology, and fostering social inclusion for visually impaired individuals.
Histological, Transcriptomic, and Functional Analyses Reveal the Role of Gibberellin in Bulbil Development in Lilium lancifolium
Authors: Du Shanshan , Wang Mengdi , Liang Jiahui , Pan Wenqiang , Sang Qianzi , Ma Yanfang , Jin Mengzhu , Zhang Mingfang , Zhang Xiuhai , Du Yunpeng
Published: October 2024

Lily bulbils, advantageous axillary organs used for asexual reproduction, have an underexplored developmental mechanism. Gibberellins are known to participate in bulbil development, but the regulatory mechanisms remain unclear. In this study, exogenous gibberellin (GA3) significantly increased the bulbil length, width, and weight by raising the endogenous gibberellin levels and elongating the scale cells. Transcriptomic analysis identified LlGA20ox2, a key gibberellin biosynthesis gene, which was upregulated during bulbil development and significantly responsive to GA3 treatment. Given the similarities in bulbil and bulblet development, we determined the roles of LlGA20ox2 using a bulblet system. Silencing LlGA20ox2 in bulblets inhibited development by reducing the cell length, while overexpression increased the bulblet length and width. In the gibberellin signaling pathway, we identified two key genes, LlGID1C and LlCIGR2. Silencing these genes resulted in phenotypes similar to LlGA20ox2, inhibiting bulblet development. Further transcriptomic analysis revealed that gibberellin-responsive genes were enriched in the glucuronate pathway, pentose phosphate pathway and galactose metabolism pathways. Most of these differentially expressed genes responded to gibberellin and were highly expressed in later stages of bulbil development, suggesting their involvement in gibberellin-regulated bulbil growth. In conclusion, we preliminarily explored the mechanisms of gibberellin regulation in bulbil development, offering significant commercial potential for new lily reproductive organs.
Allelopathic Interactions Between the Green-Tide-Forming Ulva prolifera and the Golden-Tide-Forming Sargassum horneri Under Controlled Laboratory Conditions
Authors: Sun Ruibin , Korboon Onjira , Ma Wenfei , Ren Xingyue , Wang Xiaonan , Muangmai Narongrit , Xing Qikun , Gao Xu , Li Jingyu
Published: October 2024

Harmful algal blooms (HABs) represent a significant global marine ecological disaster. In the Yellow Sea, green and golden tides often occur simultaneously or sequentially, suggesting that interspecific competition involves not only spatial and resource competition but also allelopathy. This study investigated the allelopathic interactions between Ulva prolifera and Sargassum horneri using physiological and biochemical parameters, including relative growth rate (RGR), cell ultrastructure, chlorophyll fluorescence, enzyme activity, and metabolomics analysis. The results showed that S. horneri filtrate significantly inhibited U. prolifera growth, while U. prolifera filtrate had no significant effect on S. horneri. Both algal filtrates caused cellular damage and affected photosynthesis, enzyme activities, and metabolism. However, their allelopathic responses differed: U. prolifera may rely on internal compensatory mechanisms, while S. horneri may depend on defense strategies. These findings provide insights into the dynamics of green and golden tides and support the scientific control of HABs through allelopathy.
New Horizons in Plant–Microbe Interactions
Authors: Cerny Martin , Hýsková Veronika
Published: October 2024

Theophrastus, an ancient Greek philosopher known as the “Father of Botany,” was among the first to document plant diseases, establishing an understanding of plant biotic interactions some 2300 years ago [...]
Genome-Wide Analysis of Caffeoyl-CoA-O-methyltransferase (CCoAOMT) Family Genes and the Roles of GhCCoAOMT7 in Lignin Synthesis in Cotton
Authors: Ma Lina , Wang Jin , Qiao Kaikai , Quan Yuewei , Fan Shuli , Wu Liqiang
Published: October 2024

Caffeoyl coenzyme A-O-methyltransferase (CCoAOMT) has a critical function in the lignin biosynthesis pathway. However, its functions in cotton are not clear. In this research, we observed 50 CCoAOMT genes from four cotton species, including two diploids (Gossypium arboretum, 9, and Gossypium raimondii, 8) and two tetraploids (Gossypium hirsutum, 16, and Gossypium barbadense, 17), performed bioinformatic analysis, and focused on the involvement and functions of GhCCoAOMT7 in lignin synthesis of Gossypium hirsutum. CCoAOMT proteins were divided into four subgroups based on the phylogenetic tree analysis. Motif analysis revealed that all CCoAOMT proteins possess conserved Methyltransf_3 domains, and conserved structural features were identified based on the genes’ exon-intron organization. A synteny analysis suggested that segmental duplications were the primary cause in the expansion of the CCoAOMT genes family. Transcriptomic data analysis of GhCCoAOMTs revealed that GhCCoAOMT2, GhCCoAOMT7, and GhCCoAOMT14 were highly expressed in stems. Subcellular localization experiments of GhCCoAOMT2, GhCCoAOMT7, and GhCCoAOMT14 showed that GhCCoAOMT2, GhCCoAOMT7, and GhCCoAOMT14 were localized in the nucleus and plasma membrane. However, there are no cis-regulatory elements related to lignin synthesis in the GhCCoAOMT7 gene promoter. GhCCoAOMT7 expression was inhibited by virus-induced gene silencing technology to obtain gene silencing lines, the suppression of GhCCoAOMT7 expression resulted in a 56% reduction in the lignin content in cotton stems, and the phloroglucinol staining area corresponding to the xylem was significantly decreased, indicating that GhCCoAOMT7 positively regulates lignin synthesis. Our results provided fundamental information regarding CCoAOMTs and highlighted their potential functions in cotton lignin biosynthesis and lignification.
Proteomic and Targeted Lipidomic Analyses of Fluid and Rigid Rubber Particle Membrane Domains in Guayule
Authors: Blakeslee Joshua J. , Han Eun-Hyang , Lin Yun , Lin Jinshan , Nath Seema , Zhang Liwen , Li Zhenyu , Cornish Katrina
Published: October 2024

Rubber (cis-1,4-polyisoprene) is produced in cytosolic unilamellar vesicles called rubber particles (RPs), and the protein complex responsible for this synthesis, the rubber transferase (RTase), is embedded in, or tethered to, the membranes of these RPs. Solubilized enzyme activity is very difficult to achieve because the polymerization of highly hydrophilic substrates into hydrophobic polymers requires a polar/non-polar interface and a hydrophobic compartment. Using guayule (Parthenium argentatum) as a model rubber-producing species, we optimized methods to isolate RP unilamellear membranes and then a subset of membrane microdomains (detergent-resistant membranes) likely to contain protein complexes such as RTase. The phospholipid and sterol composition of these membranes and microdomains were analyzed using thin-layer chromatography (TLC) and liquid chromatography tandem mass spectroscopy (LC-MS/MS). Our data indicate that RP membranes consist predominantly of phosphatidic acid-containing membrane microdomains (DRMs or “lipid rafts”). Proteomic analyses of guayule RP membranes and membrane microdomains identified 80 putative membrane proteins covering 30 functional categories. From this population, we have tentatively identified several proteins in multiple functional domains associated with membrane microdomains which may be critical to RTase function. Definition of the mechanisms underlying rubber synthesis will provide targets for both metabolic engineering and breeding strategies designed to increase natural rubber production in latex-producing species.
Mechanisms of Cannabis Growth Promotion by Bacillus velezensis S141
Authors: Aunkam Phirom , Sibponkrung Surachat , Limkul Sirawich , Seabkongseng Tuangrak , Mahanil Kanjana , Umnajkitikorn Kamolchanok , Boonkerd Nantakorn , Teaumroong Neung , Sato Shusei , Tittabutr Panlada , Boonchuen Pakpoom
Published: October 2024

Cannabis sativa L. has a variety of uses, including fiber production, food, oil, and medicine. In response to environmental concerns regarding chemical fertilizers, Bacillus velezensis S141 was examined as a plant-growth-promoting bacterium (PGPB) for cannabis. This study evaluated the effects of S141 on cannabis growth and utilized transcriptomic analysis to identify the responsive pathways. Inoculation with S141 significantly increased growth in laboratory and field environments, with most of the bacteria residing in the leaves, followed by the stems and roots, as determined by quantitative polymerase chain reaction (qPCR). Transcriptomic analysis revealed 976 differentially expressed genes. Upregulated genes were associated with metabolism, cellular processes, and catalytic activities, especially in the biosynthesis of phenylpropanoid, plant–pathogen interactions, and hormone signaling pathways. S141 mutants deficient in the production of auxin and cytokinin displayed reduced growth enhancement, which affirmed the roles of these hormones in cannabis development. These findings emphasize the potential of S141 as a sustainable growth promoter for cannabis and provide insights into the underlying pathways it influences.
The Adsorption of Durene and Prehnitene on Metal–Organic Frameworks
Authors: Wang Tianyou , Wu Yanyang , Rao Jiabo , Wang Xudong , Wu Bin , Chen Kui , Ji Lijun
Published: October 2024

Tetratoluene has the following three isomers: durene (DR), prehnitene (PR), and isodurene (IR). DR and PR often coexist during the separation of C10 heavy aromatics at different levels. They are both important organic chemical raw materials and their separation is the key to the high-efficiency industrial utilization of C10 heavy aromatics. In this paper, six metal–organic frameworks (MOFs), including ZU-61, MIL-101, UIO-66, UIO-66-NH2, Mg-MOF-74, and MIL-53(Al), were used as the adsorbents of DR and PR. Their skeletons were structurally optimized using VASP software (latest v. 6.4.3). The adsorption capacity and isosteric heats of both pure components and mixtures (the molar ratio was 1:1) in gas were calculated using Grand Canonical Monte Carlo simulation from 10 kPa to 300 kPa at 298 K. The results indicated that all adsorption processes were physical. ZU-61, UIO-66, UIO-66-NH2, and Mg-MOF-74 presented suitable capacity differences for DR and PR at 300 kPa. The selectivity values of these frameworks were all above 1.5. Thus, the four MOFs were prepared using the solvothermal method and characterized by SEM and XRD. Then, the competitive adsorption of DR and PR in liquid on the four MOFs was carried out as well. The results showed good agreement with the simulation in general, with a lower adsorption attained capacity due to the different phase states of both DR and PR. This study can guide the separation of tetratoluene isomers in C10 heavy aromatics.
Application of Hybrid Attention Mechanisms in Lithological Classification with Multisource Data: A Case Study from the Altay Orogenic Belt
Authors: Li Dong , Wang Jinlin , Zhou Kefa , Bi Jiantao , Zhang Qing , Wang Wei , Qu Guangjun , Li Chao , Qiu Heshun , Liao Tao , Zhao Chong , Lu Yingpeng
Published: October 2024

Multisource data fusion technology integrates the strengths of various data sources, addressing the limitations of relying on a single source. Therefore, it has been widely applied in fields such as lithological classification and mineral exploration. However, traditional deep learning algorithms fail to distinguish the importance of different features effectively during fusion, leading to insufficient focus in the model. To address this issue, this paper introduces a ResHA network based on a hybrid attention mechanism to fuse features from ASTER remote sensing images, geochemical data, and DEM data. A case study was conducted in the Altay Orogenic Belt to demonstrate the lithological classification process. This study explored the impact of the submodule order on the hybrid attention mechanism and compared the results with those of MLP, KNN, RF, and SVM models. The experimental results show that (1) the ResHA network with hybrid attention mechanisms assigned reasonable weights to the feature sets, allowing the model to focus on key features closely related to the task. This resulted in a 7.99% improvement in classification accuracy compared with that of traditional models, significantly increasing the precision of lithological classification. (2) The combination of channel attention followed by spatial attention achieved the highest overall accuracy, 98.06%.
Video Game-Based Trunk Exercises for Rehabilitation in Chronic Stroke Survivors: A Mixed-Methods Feasibility Study
Authors: Alhwoaimel Norah A. , Hughes Ann-Marie , Warner Martin , Alenazi Aqeel M. , Alshehri Mohammed M. , Alqahtani Bader A. , Alhowimel Ahmed S. , Wagland Richard , Brown Simon , Turk Ruth
Published: October 2024

Aim: To assess the feasibility of video game-based trunk exercises using the Valedo® system in a chronic stroke population. Method: Ten chronic stroke survivors (eight males and two females, mean age 63 ± 15 years) were asked to complete 18 intervention sessions, each lasting 45 min., over 6–8 weeks. Feasibility was evaluated quantitatively using the Psychosocial Impact of Assistive Devices Scale (PIADS) as well as through recruitment, retention, adherence, and safety measures. Qualitative data on feasibility were collected through post-intervention semi-structured interviews. Descriptive analysis was used to summarize participant characteristics, recruitment, retention, and adherence. Qualitative data were analyzed using thematic analysis of the interviews. Results: Twelve stroke survivors were recruited from Southampton (United Kingdom) and Riyadh (Kingdom of Saudi Arabia), with two participants dropping out after the baseline assessment session. The remaining ten participants completed the study with a mean adherence of 96.11% to the planned sessions. No serious adverse effects were reported, however, four participants did experience trunk muscle tightness and fatigue. Post-intervention interviews revealed that participants encountered some physical and cognitive challenges while playing the Valedo video games. However, they felt that the implementation of trunk exercises using video games was safe, as the exercises were performed in a secure environment and in safe positions. Conclusions: The findings suggest that the Valedo system is feasible for delivering trunk exercises to chronic stroke survivors. Several factors should be considered when implementing this type of intervention in the future.
A Dual-Band Patch Antenna with Combined Self-Decoupling and Filtering Properties and Its Application in Dual/Squad-Band Two-Element MIMO Array
Authors: Lv Jun-Yi , Zhang Jun-Ming , Lv Peng-Fei , Xu Li-Xin
Published: October 2024

This paper proposes a dual-band patch antenna with combined self-decoupling and filtering properties, designed to suppress mutual coupling between two antenna elements both within the same dual-band and across different dual-bands. Initially, a dual-band aperture-coupled filtering patch antenna is designed, featuring a forked short-circuited SIR feedline with a quarter-wavelength open-ended stub and a U-shaped patch with two U-slots, which generate three controllable radiation nulls while introducing two additional resonant modes. The design steps are also provided in detail. Subsequently, the low mutual coupling phenomenon of two vertically placed aperture-coupled patch antennas is investigated, successfully developing a high-isolated dual-band two-element MIMO array I. Furthermore, the other quad-band two-element MIMO array II is designed, which utilizes the filtering response to significantly reduce mutual coupling across four bands. Finally, a dual-band filtering patch antenna element and two two-element MIMO arrays are fabricated and measured. The measurements and simulations validate the antenna’s low mutual coupling performance in multi-band MIMO arrays and demonstrate its strong potential for future wireless communication applications.
Blockchain Factors in the Design of Smart-Media for E-Healthcare Management
Authors: Shah Dhaneshwar , Rani Sunanda , Shoukat Khadija , Kalsoom Habiba , Shoukat Muhammad Usman , Almujibah Hamad , Liao Shengxiao
Published: October 2024

According to the current situation of deep aging globally, how to provide low-cost and high-quality medical services has become a problem that the whole society needs to consider. To address these challenges, we propose an e-healthcare management system leveraging the integration of the Internet of Things (IoT) and blockchain technologies. Our system aims to provide comprehensive, reliable, and secure one-stop services for patients. Specifically, we introduce a blockchain-based searchable encryption scheme for decentralized storage and real-time updates of electronic health records (EHRs). This approach ensures secure and efficient data traceability across medical equipment, drug supply chains, patient health monitoring, and medical big data management. By improving information processing capabilities, our system aspires to advance the digital transformation of e-healthcare services.
Adaptive Fault-Tolerant Tracking Control for Multi-Joint Robot Manipulators via Neural Network-Based Synchronization
Authors: Le Quang Dan , Yang Erfu
Published: October 2024

In this paper, adaptive fault-tolerant control for multi-joint robot manipulators is proposed through the combination of synchronous techniques and neural networks. By using a synchronization technique, the position error at each joint simultaneously approaches zero during convergence due to the constraints imposed by the synchronization controller. This aspect is particularly important in fault-tolerant control, as it enables the robot to rapidly and effectively reduce the impact of faults, ensuring the performance of the robot when faults occur. Additionally, the neural network technique is used to compensate for uncertainty, disturbances, and faults in the system via online updating. Firstly, novel robust synchronous control for a robot manipulator based on terminal sliding mode control is presented. Subsequently, a combination of the novel synchronous control and neural network is proposed to enhance the fault tolerance of the robot manipulator. Finally, simulation results for a 3-DOF robot manipulator are presented to demonstrate the effectiveness of the proposed controller in comparison to traditional control techniques.
Inspiratory Muscle Performance Is Significantly Related to Acceleration and Deceleration of Isokinetic Knee Extension and Flexion in Division 1 Collegiate Women Soccer Players: A Pilot Study
Authors: Kasa Tiffany , Feigenbaum Luis A. , Ruiz Jeffrey T. , Cohen Meryl , Rapicavoli Julia L. , Dibble Nicholas H. , Poliszuk Robert O. , Jones Rebecca , Cahalin Lawrence P.
Published: October 2024

This study examines the relationship between inspiratory muscle performance (IMP) using the Test of Incremental Respiratory Endurance (TIRE), knee extension (EXT) and flexion (FLEX) acceleration (ACCEL) and deceleration (DECEL) times on an isokinetic dynamometer (ID), and performance of the Yo-Yo Intermittent Recovery Test Level 1 (YYIRT1) among thirteen Division I collegiate women soccer players (D1CWSP). Knee EXT and FLEX were tested at three velocities (60°/s, 180°/s, and 300°/s) using ID, while IMP was measured using maximal inspiratory pressure (MIP) and sustained maximal inspiratory pressure (SMIP). Significant negative correlations were found between MIP and the ACCEL time of FLEX at 60°/s and 300°/s, as well as between MIP and the DECEL time of EXT at 180°/s. Additionally, SMIP was negatively correlated with ACCEL times of FLEX at 180°/s and 300°/s and with DECEL times for both EXT and FLEX at 300°/s. These results suggest that greater IMP is linked to faster ACCEL and DECEL times in knee EXT and FLEX, indicating a role of IMP in enhancing muscle recruitment and performance. The only significant correlation with the YYIRT1 performance was observed between the YYIRT accumulated distance and the ACCEL time of FLEX at 180°/s. These findings highlight a physiological mechanism whereby IMP may contribute to improved dynamic muscle performance, though further research is needed to explore its implications for overall athletic performance.
Is Sharing One’s Personal Story of Victimization Preferred? Incarcerated Women’s Perspectives on Group Treatment for Sexual Trauma
Authors: Karlsson Marie , Zielinski Melissa , Bridges Ana
Published: October 2024

Treatment preferences are an important part of evidence-based practice and have been shown to affect treatment outcomes. In this two-part study, incarcerated women were asked about their preferences for two versions of a trauma-focused group treatment: one that requires sharing their personal memory of sexual victimization (Sharing Required) and one that does not (Sharing Not Required). Study 1 enrolled 88 non-treatment seeking women who evaluated the treatments based on descriptions of the groups. Study 2 was a partially randomized patient preference trial with 85 treatment-seeking women who either agreed to be randomly assigned to one of the two therapy groups or declined randomization and instead were directly assigned to their preferred therapy. Participants in Study 1 evaluated the Sharing Not Required condition more favorably (p < 0.001, Cohen’s d = 0.39). However, the results were affected by PTSD symptom severity as those above the clinical cutoff for probable PTSD evaluated both group treatments more favorably than those below the cutoff (ps < 0.05, Cohen’s ds ≥ 0.46). Study 2 found no significant difference between the proportion of participants who chose Sharing Required, Sharing Not Required, or had no personal preference, and the results did not differ by PTSD symptom severity (ps ≥ 0.70). Outcomes suggest that a variety of forms of trauma-focused therapy may be acceptable to incarcerated women, including those that involve personal narration of trauma memories and those that do not.
Morphological and Cytogenetic Responses of In Vitro-Grown Grapevine (Vitis vinifera L.) Plants from “Touriga Franca”, “Touriga Nacional” and “Viosinho” Varieties Under Water Stress
Authors: Carvalho Ana , Crisóstomo Christina , Leal Fernanda , Lima-Brito José
Published: October 2024

According to the climate projections, drought will increase in frequency and severity. Since water stress (WS) impacts a grapevine’s physiology and yield negatively, the evaluation and selection of tolerant genotypes are needed. To analyse the WS effects on the morphology and cell division of three grapevines (Vitis vinifera L.) varieties, “Touriga Franca” (TF), “Touriga Nacional” (TN) and “Viosinho” (VS), in vitro-grown plants were exposed to 10% polyethylene glycol 6000 (PEG) (−0.4 MPa) or 20% PEG (−0.8 MPa), incorporated in the culture medium, for four weeks. Control plants were kept in culture media without PEG. The VS and TN plants showed the highest mean numbers of nodes, shoots and leaves and average mitotic indexes under 20% PEG. The TF and TN plants showed the lowest frequencies of mitotic anomalies under 10% PEG. The VS plant growth was less affected by WS, but TF and TN presented more regular mitosis under moderate WS. Globally, in vitro culture constitutes a cost-effective experimental system for studying grapevine responses to WS and the preliminary selection of resilient genotypes. These approaches could be applied to study plant responses to other abiotic stresses based on additional evaluation techniques (e.g., transcriptional analyses or genome-wide association studies).
Educating in and for Degrowth: Training Future Generations to Prevent Environmental Collapse
Authors: Díez-Gutiérrez Enrique-Javier , Trujillo Vargas José Jesús , Palomo-Cermeño Eva , Perlado-Lamo de Espinosa Ignacio , García-Salas Luisa-María , Romero Acosta Kelly , Mateos-Toro Luis-Miguel , Pérez-Robles Antonio
Published: October 2024

This research has been developed through a literature review on the importance of and current approach in the education system to the present environmental and ecosystemic crisis and the training of future generations in degrowth in the Spanish education system. To this end, a systematic literature review (SLR) has been carried out following the standards of the PRISMA declaration. In total, 40 articles published between January 2005 and March 2024 were selected from the following databases: Scopus, Dialnet, Web of Science and Scielo. The findings show it is a relevant topic in school education as a concern, but it is not reflected in educational practice; that it has been incorporated into the curriculum, but sporadically, decontextualised and more focused on ‘sustainable development’; also, it lacks critical questioning of the unlimited growth and consumption model that capitalism entails. The study concludes that it is crucial to incorporate degrowth in a transversal way in education at all schooling levels, and to reform the curricula of the faculties of education in all universities so that the pedagogy of degrowth is a priority in the training of future teachers.
Effects of High-Density Mixed Planting in Artificial Grassland on Microbial Community
Authors: Tao Ziwei , Li Jinjuan , Li Hui , Du Guozhen
Published: October 2024

The construction level of artificial grassland is an important index of the development degree of grassland animal husbandry. Therefore, improving the productivity level of artificial grassland and promoting the sustainable utilization of artificial grassland have become important tasks that need to be urgently addressed. There have been numerous studies on the effects of monoculture on the soil microbial community structure in artificial grassland, but there is limited research on the effects of mixed sowing on the soil microbial community structure and the related patterns. In this study, Elymus nutans (En), Festuca sinensis (Fs), Avena sativa (As), and Poa pratensis (Pp) were used as common herbage materials in an alpine grassland pastoral area of the eastern Tibetan Plateau. Multi-density monoculture and mixed seeding were employed to establish artificial grassland communities with varying structures. By comparing the soil microbial community structure of the differently treated artificial planting grass, degraded grassland with bald spots, and natural grassland, it was confirmed that plant community diversity significantly influences the microbial community structure. The high-density planting treatment of multiple forage grasses had a more pronounced impact on the soil microbial community structure compared to that of the high-density planting treatment of a single variety of forage grass. The soil microbial community diversity index of the four mixed-planting treatments was higher than those of the other artificial grassland treatments and the natural grassland treatments, and the soil microbial community structure was most similar to that of the natural grassland. Avena sativa planting increased the abundance of Actinobacteria and Basidiomycota and decreased the number of Acidobacteria by increasing the soil pH value. The AFP (As+Fs+Pp) treatment reduced the proportion of Mortierellomycota in the soil by decreasing the content of available phosphorus. The AEFP (As+En+Fs+Pp) treatment increased the number of Proteobacteria by raising the soil total phosphorus content and reduced the abundance of Acidobacteria by lowering the soil pH value. Additionally, a machine learning method was used to evaluate the comprehensive performance of 21 artificial grassland treatments on nine soil physical and chemical properties. It was found that the AEFP mixed-planting and high-density planting treatments had the greatest improvement effect on the nine soil physical and chemical properties, which was conducive to sustainable land use.
The Influence of Green Demarketing on Brand Credibility, Green Authenticity, and Greenwashing in the Food Industry
Authors: Azazz Alaa M. S. , Elshaer Ibrahim A. , Thani Abdulaziz Al , Algezawy Mohamed , Mahrous Abeer A. , Mansour Mahmoud A. , Abdel Moneim Azza , Fayyad Sameh
Published: October 2024

In the age where environmental sustainability issues are progressively prioritized, green demarketing has risen as a strategic choice for organizations aiming to decrease customer demand for unsustainable services/products and stimulate more eco-friendly substitutes. This paper investigates the impact of green demarketing on brand credibility, green authenticity, and perceptions of greenwashing. This paper examines how restaurants that are engaged in green demarketing practices are perceived with regard to their commitment toward the environment and whether such practices improve or reduce a brand’s credibility. Moreover, this study explores green authenticity and explores how an organization’s brand looks in its sustainability practices when employing demarketing activities. The proper consequences of greenwashing, where customers might perceive these tactics as insincere or misleading, are also significantly explored. By employing a survey research method, 414 restaurant customers were targeted, and the gathered data were analyzed employing partial least square structural equation modeling (PLS-SEM). This study’s results might contribute to the increasing interest in sustainable marketing activities and deliver practical implications for restaurants aiming to navigate the complex multi-dynamics of ecofriendly responsibility and consumer credibility.