bluuebunny/crossref_metadata_2025 huggingface dataset:
Performance of Bituminous Binder Modified with Recycled Plastic Pellets
Authors: Soliman Haithem , Osei Paul , Shalaby Ahmed
Published: October 2023

Finding beneficial uses for waste plastics has been an environmental challenge for municipalities. A limited number of studies have investigated the performance of asphalt mixtures containing plastic waste in cold regions that experience freeze-thaw cycling. The objective of this study is to evaluate the impact of adding two types of recycled plastic pellets on the high- and low-temperature performance of bituminous binders. Nylon-based (NP) and polyester-based (PP) recycled plastic pellets were used in this study. A PG 58-28 bituminous binder was modified by different dosages of NP and PP plastic pellets. The impact of adding Elvaloy copolymer and polyphosphoric acid on the modified binders was also investigated. Results showed that using recycled plastic pellets as a modifier for bituminous binders improved their elastic response and rutting resistance without affecting their low-temperature performance. The PP modifier showed better elastic behavior and rutting resistance than the NP modifier.
Evolution of Microstructure and Texture in Grain-Oriented 6.5% Si Steel Processed by Rolling with Intrinsic Inhibitors and Additional Inhibitors
Authors: Liang Ruiyang , Sun Chengqian , Li Qingchun
Published: October 2023

A grain-oriented steel containing 6.5% Si, characterized by a notable Goss texture, was effectively manufactured through the rolling technique, incorporating both intrinsic inhibitors and additional inhibitors. This investigation focuses on tracking the development of texture and magnetic properties during the manufacturing process and delineates the mechanism underlying secondary recrystallization. The empirical findings clearly demonstrated the significant influence of nitriding duration and quantity on the secondary recrystallization process. In instances where additional nitrogen is absent, the intrinsic inhibitors alone do not lead to secondary recrystallization. However, when the nitriding duration is 90 s and the nitriding amount is 185 ppm, a complete secondary recrystallization structure with a strong Goss texture enables the finished products have excellent magnetic properties. The preferential growth of Goss grains is mainly governed by the enhanced mobility of high-energy (HE) grain boundaries. With the increase in annealing temperature, the occurrence of 20°–45° HE grain boundaries with Goss grains becomes more progressively frequent. At the secondary recrystallization temperature of 1000 °C, the frequency of 20°–45° HE grain boundaries with Goss grains reaches 62.7%, providing favorable conditions for the abnormal growth of Goss grains. This results in a secondary recrystallization structure predominantly characterized by a strong Goss texture. In light of these observations, the present study provides fundamental theoretical insights and serves as a valuable procedural guideline for the industrial manufacturing of 6.5% Si grain-oriented electrical steels.
Phase Transitions in Boron Carbide
Authors: Werheit Helmut
Published: October 2023

The idealized rhombohedral unit cell of boron carbide is formed by a 12-atom icosahedron and a 3-atom linear chain. Phase transitions are second order and caused by the exchange of B and C sites or by vacancies in the structure. Nevertheless, the impact of such minimal structural changes on the properties can be significant. As the X-ray scattering cross sections of B and C isotopes are very similar, the capability of X-ray fine structure investigation is substantially restricted. Phonon spectroscopy helps close this gap as the frequency and strength of phonons sensitively depend on the bonding force and mass of the vibrating atoms concerned. Phase transitions known to date have been identified due to significant changes of properties: (1) The phase transition near the chemical composition B8C by clear change of the electronic structure; (2) the endothermic temperature-dependent phase transition at 712 K according to the change of specific heat; (3) the high-pressure phase transition at 33.2 GPa by the drastic change of optical appearance from opacity to transparency. These phase transitions affect IR- and Raman-active phonons and other solid-state properties. The phase transitions at B~8C and 712 K mean that a well-defined distorted structure is converted into another one. In the high-pressure phase transition, an apparently well-defined distorted structure changes into a highly ordered one. In all these cases, the distribution of polar C atoms in the icosahedra plays a crucial role.
Empirical Filtering-Based Artificial Intelligence Learning Diagnosis of Series DC Arc Faults in Time Domains
Authors: Dang Hoang-Long , Kwak Sangshin , Choi Seungdeog
Published: October 2023

Direct current (DC) networks play a pivotal role in the growing integration of renewable energy sources. However, the occurrence of DC arc faults can introduce disruptions and pose fire hazards within these networks. In order to ensure both safety and optimal functionality, it becomes imperative to comprehend the characteristics of DC arc faults and implement a dependable detection system. This paper introduces an innovative arc fault detection algorithm that leverages current filtering based on the empirical rule in conjunction with intelligent machine learning techniques. The core of this approach involves the sampling and subsequent filtration of current using the empirical rule. This filtering process effectively amplifies the distinctions between normal and arcing states, thereby enhancing the overall performance of the intelligent learning techniques integrated into the system. Furthermore, this proposed diagnosis scheme requires only the signal from the current sensor, which reduces the complexity of the diagnosis scheme. The results obtained from the detection process serve to affirm the effectiveness and reliability of the proposed DC arc fault diagnosis scheme.
Adaptive Finite-Time Fuzzy Control for Uncertain Nonlinear Systems with Asymmetric Full-State Constraints
Authors: Hou Yinlong , Xu Xiaoling , Liu Ruixia , Bai Xiangyun , Liu Hui
Published: October 2023

This paper studies the adaptive finite-time fuzzy control issue associated with uncertain nonlinear systems that exhibit asymmetric constraints on the full state. A distinct function, constrained by nonlinear states, is designed to mitigate the excessive breach of these full-state boundaries. Unlike the standard barrier Lyapunov function (BLF) method, this approach solves symmetric and asymmetric full-state constraints without modifying the controller structure, and it does not require any additional assumptions about virtual control to be met. Simultaneously employing approximating functions using fuzzy logic systems and incorporating dynamic surface control technology integrated with a first-order filter, the unknown nonlinear functions emanating from the suggested controller strategy are estimated. Additionally, this approach addresses the prevalent problem of complexity explosion observed in conventional backstepping techniques. An adaptive finite-time fuzzy tracking control strategy is introduced, ensuring that all signals and tracking errors of the controlled system remain bounded in finite time. Finally, two simulation examples are given to illustrate the effectiveness of the proposed control scheme, confirming that all states remain within the predefined regions.
Existence of Positive Ground States of Nonlocal Nonlinear Schrödinger Equations
Authors: Zhang Yong-Chao , Lu Yao
Published: October 2023

We investigate ground states of a (nonlocal) nonlinear Schrödinger equation which generalizes classical (fractional, relativistic, etc.) Schrödinger equations, so that we extend relevant results and study common properties of these equations in a uniform way. To obtain the existence of ground states, we first solve a minimization problem and then prove that the solution of the minimization problem is a ground state of the equation. After examining the regularity of the solutions to the equation, we demonstrate that any ground state is sign-definite.
Normalization Method as a Potent Tool for Grasping Linear and Nonlinear Systems in Physics and Soil Mechanics
Authors: Conesa Manuel , Sánchez-Pérez Juan Francisco , García-Ros Gonzalo , Castro Enrique , Valenzuela Julio
Published: October 2023

To address physical problems that require solving differential equations, both linear and nonlinear analytical methods are preferred when possible, but numerical methods are utilized when necessary. In this study, the normalization technique is established, which is a simple mathematical approach that requires only basic manipulation of the governing equations to obtain valuable information about the solution. The methodology of this technique involves adopting appropriate references to obtain the dimensionless form of the governing equation, after which the terms of the equation are balanced, obtaining the dimensionless monomials governing the solution. Thorough knowledge of the physical processes involved is necessary to find the best references. The main advantages of this technique are the simplicity of the methodology, the acquisition of valuable information about the solution without the need for complex mathematical calculations, and its applicability to nonlinear problems. However, it is important to consider the difficulty in selecting appropriate references in more complex scenarios. This study applies this normalization methodology to different scenarios, showing how choosing appropriate references lead to the independent dimensionless monomials. Once obtained, it was possible to identify different situations concerning the value of monomials. It will be when they are close to unity, and therefore normalized, when they fundamentally affect the solution of the problem. Finally, we present two cases, one linear and one complex, about the application of normalization to the challenging problem of soil consolidation in ground engineering, illustrating how the technique was used to obtain the solution and its many advantages.
Complexity-Effective Joint Detection of Physical Cell Identity and Integer Frequency Offset in 5G New Radio Communication Systems
Authors: You Young-Hwan , Jung Yong-An , Lee Sung-Hun , Choi Sung-Chan , Hwang Intae
Published: October 2023

This paper presents a simplified joint synchronization scheme for the integer carrier frequency offset and physical cell identity using a primary synchronization signal (PSS) in 5G new radio (NR) communication systems. We demonstrate the efficiency of our proposed NR-PSS synchronization scheme by deriving its simplified implementation, which exploits the near-zero autocorrelation feature between cyclically shifted NR-PSS symbols. As a figure of merit, we compute the probability of detection failure of the proposed NR-PSS synchronization scheme and validate its accuracy via simulations. To illustrate the benefits and limitations of the proposed NR-PSS synchronization scheme, we compare it with the conventional NR-PSS synchronization scheme, considering factors such as detection performance and computation complexity. Numerical results indicate that, regardless of the channel environment, the proposed NR-PSS synchronization scheme achieves a significant reduction in arithmetic complexity while maintaining the same detection capability as existing NR-PSS synchronization schemes.
Prevalence Rates of Arterial Hypertension According to the Threshold Criteria of 140/90 or 130/80 mmHg and Associated Cardiometabolic and Renal Factors: SIMETAP-HTN Study
Authors: Pallarés-Carratalá Vicente , Ruiz-García Antonio , Serrano-Cumplido Adalberto , Arranz-Martínez Ezequiel , Divisón-Garrote Juan Antonio , Moyá-Amengual Ana , Escobar-Cervantes Carlos , Barrios Vivencio
Published: October 2023

Background and objectives: Arterial hypertension (HTN) is the leading preventable cause of atherosclerotic cardiovascular diseases (ASCVD) and death from all causes. This study aimed to determine the prevalence rates of HTN diagnosed according to the threshold diagnostic criteria 130/80 mmHg and 140/90 mmHg, to compare blood pressure (BP) control, and to evaluate their associations with cardiovascular diseases and cardiometabolic and renal risk factors. Materials and Methods: This was a cross-sectional observational study conducted in primary care with a population-based random sample: 6588 people aged 18.0–102.8 years. Crude and adjusted prevalence rates of HTN were calculated. BP control was compared in HTN patients with and without ASCVD or chronic kidney disease (CKD). Their associations with cardiovascular diseases and cardiometabolic and renal factors were assessed using bivariate and multivariate analysis. Results: Adjusted prevalence rates of HTN diagnosed according to 140/90 and 130/90 criteria were 30.9% (32.9% male; 29.7% female) and 54.9% (63.2% male; 49.3% female), respectively. BP < 130/80 mmHg was achieved in 60.5% of HTN patients without ASCVD or CKD according to 140/90 criterion, and 65.5% according to 130/80 criterion. This BP-control was achieved in 70% of HTN patients with ASCVD and 71% with CKD, according to both criteria. Coronary heart disease (CHD), heart failure, atrial fibrillation, stroke, diabetes, prediabetes, low glomerular filtration rate (eGFR), hyperuricemia, hypercholesterolemia, obesity, overweight, and increased waist-to-height ratio were independently associated with HTN according to both criteria. Conclusions: Almost a third of the adult population has HTN according to the 140/90 criterion, and more than half according to the 130/90 criterion, with a higher prevalence in men. The main clinical conditions associated with HTN were heart failure, diabetes, CHD, low eGFR, and obesity.
Effect of Composition and Thermal Treatments on Mechanical Properties and Applications of Quenching and Partitioning Steels
Authors: Tedesco Michele Maria , Caro Daniele De , Rizzi Paola , Baricco Marcello
Published: October 2023

In this review, we compared nine different chemical compositions and several heat treatments applied on quenching and partitioning steels investigated in the literature. All of these parameters were correlated to the reported mechanical properties (e.g., yield, tensile strength, elongation and toughness). Sustainability and a circular economy approach for applications in the automotive sector were also considered, providing hints for lightweighting in the car industry and the next possible steps to improve the decarbonization of the steel industry.
Review on Laser Shock Peening Effect on Fatigue of Powder Bed Fusion Materials
Authors: Bumba Francisco , Morais Paulo , Batalha Rodolfo , Anes Vitor , Reis Luis
Published: October 2023

The ability to manufacture parts with complex geometry by sending a model from CAD directly to the manufacturing machine has attracted much attention in the industry, driving the development of additive manufacturing technology. However, studies have shown that components manufactured using additive manufacturing technology have several problems, namely high tensile residual stresses, cracks, and voids, which are known to have a major impact on material performance (in service). Therefore, various post-treatment methods have been developed to address these drawbacks. Among the post-treatment techniques, laser shock peening (LSP) is currently considered one of the most efficient post-treatment technologies for improving the mechanical properties of materials. In practice, LSP is responsible for eliminating unfavorable tensile residual stresses and generating compressive residual stresses (CRS), which result in higher resistance to crack initiation and propagation, thus increasing component life. However, since CRS depends on many parameters, the optimization of LSP parameters remains a challenge. In this paper, a general overview of AM and LSP technology is first provided. It then describes which parameters have a greater influence during powder bed melting and LSP processing and how they affect the microstructure and mechanical properties of the material. Experimental, numerical, and analytical optimization approaches are also presented, and their results are discussed. Finally, a performance evaluation of the LSP technique in powder bed melting of metallic materials is presented. It is expected that the analysis presented in this review will stimulate further studies on the optimization of parameters via experimental, numerical, and perhaps analytical approaches that have not been well studied so far.
Effect of Mechanical Alloying on the Dissolution of the Elemental Mn and Al-Mn Compound in Aluminum
Authors: Yakovtseva Olga A. , Emelina Nadezhda B. , Mochugovskiy Andrey G. , Bazlov Andrey I. , Prosviryakov Alexey S. , Mikhaylovskaya Anastasia V.
Published: October 2023

The grain boundary, solid solution, and precipitation strengthening mechanisms are important for controlling the mechanical properties of Al-based alloys. Due to severe plastic deformation, mechanical alloying refines grain structure to a nanoscale level which leads to a strong increase in solute content and the related strengthening effect of solute atoms and secondary-phase precipitates. This study analyzed the elemental Mn and Al6Mn phase dissolution in Al during high-energy ball milling. For this purpose, XRD data, microstructure, and hardness evolutions were compared for two Al—5.2 at% Mn alloys prepared by mechanical alloying using elemental Al and Mn powders and a pre-melted master alloy. In the two-phase master alloy, containing the Al solid solution and the Al6Mn phase, the strain accumulation, grain refinement, solid solution supersaturation, and milling-induced hardening effects were facilitated. Both elemental Mn and intermetallic compound were dissolved during mechanical alloying, and the maximum solute content was near 3.1 at% Mn. A fine crystalline size of ~25 nm and the maximum Mn solute content were observed after milling of elemental powders and the master alloy for 60 h and 20 h, respectively. The microhardness of ~3 GPa corresponded to a ~3.1% solute Mn content, and the microhardness increased to ~5 GPa after long–term milling due to precipitation strengthening effect of the secondary Al6Mn phase in the master alloy.
Vertical GaN MOSFET Power Devices
Authors: Langpoklakpam Catherine , Liu An-Chen , Hsiao Yi-Kai , Lin Chun-Hsiung , Kuo Hao-Chung
Published: October 2023

Gallium nitride (GaN) possesses remarkable characteristics such as a wide bandgap, high critical electric field, robust antiradiation properties, and a high saturation velocity for high-power devices. These attributes position GaN as a pivotal material for the development of power devices. Among the various GaN-based devices, vertical GaN MOSFETs stand out for their numerous advantages over their silicon MOSFET counterparts. These advantages encompass high-power device applications. This review provides a concise overview of their significance and explores their distinctive architectures. Additionally, it delves into the advantages of vertical GaN MOSFETs and highlights their recent advancements. In conclusion, the review addresses methods to enhance the breakdown voltage of vertical GaN devices. This comprehensive perspective underscores the pivotal role of vertical GaN MOSFETs in the realm of power electronics and their continual progress.
Comparing Methods for Microplastic Quantification Using the Danube as a Model
Authors: Kiefer Tim , Knoll Martin , Fath Andreas
Published: October 2023

This study investigates the impact different mesh-sized filtration methods have on the amount of detected microplastics in the surface water of the Danube River delta. Further, the distribution of microplastics in different size categories (20 µm, 65 µm, 105 µm) and in the water column (0 m, 3 m, 6 m) was analyzed. Our findings show that the Danube River carries 46 p∙L−1 (microplastic particles per liter) with a size larger than 105 µm, 95 p∙L−1 larger than 65 µm and 2677 p∙L−1 that are larger than 20 µm. This suggests a negative logarithmic correlation between mesh size and particle amount. The most abundant polymer throughout all samples was polyethylene terephthalate, followed by polytetrafluorethylene. Overall, the data shows that different sampling methods cannot be compared directly. Further research is needed to find correlations in particle sizes for better comparison between different sampling methods.
Petrological, Geochemical and Chronological Characteristics of Dolomites in the Permian Maokou Formation and Constraints to the Reservoir Genesis, Central Sichuan Basin, China
Authors: Bai Xuejing , Zheng Jianfeng , Dai Kun , Hong Shuxin , Duan Junmao , Liu Yunmiao
Published: October 2023

The Middle Permian Maokou Formation in the Sichuan Basin has huge resources and is an important target for natural gas exploration. In recent years, significant exploration breakthroughs have been made in the dolomite field of member Mao-2 in central Sichuan, and the gas production of several wells has exceeded 1 × 106 m3/d, indicating promising prospects for exploration. However, the origin of the dolomite reservoir in member Mao-2 remains ambiguous, which restricts the accurate prediction of favorable reservoirs. This study focuses on drilling in the Hechuan area as its research object, by using a detailed description of the cores from member Mao-2 of seven wells; samples were selected for tests of the degree of dolomite cation ordering, stable carbon and oxygen isotopic compositions, strontium isotopic composition, rare earth elements, LA-ICP-MS element mapping and U-Pb dating. It is clarified that: (1) The crystalline dolomite of member Mao-2 in the Hechuan area is the main reservoir rock, and the heterogeneous vugs and fractures are the main reservoir space. The dolomite in member Mao-2 has been characterized by a low degree of cation ordering value (avg. 0.59), with values of δ13C (avg. 3.87‰), δ18O (avg. −7.15‰) and 87Sr/86Sr (avg. 0.707474) having similar geochemical characteristics to Middle Permian seawater; the REEs normalized distribution patterns have similar characteristics to limestone; and the U-Pb age (261.0~262.0 Ma) corresponds to the age in the Capitanian stage of the Permian Guadalupian Series. (2) Petrological studies show that member Mao-2 has vertical karstification zonation characteristics; syngenetic karstification controls the formation of a large-scale fracture-cave system in the phreatic zone; the dolomitization of sediment in the fracture-cave system occurred during the penecontemporaneous period with locally restricted seawater. (3) The main controlling factors of the reservoir were syngenetic karstification, early dolomitization and hydrothermal dissolution related to Emei taphrogenesis. The research results are of great significance for dolomite reservoir prediction; the highlands of paleogeomorphology with syndepositional faults are favorable areas for dolomite reservoirs.
Measuring the Residual Levels of Fenpyroximate and Its Z-Isomer in Citrus Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry and Assessing the Related Dietary Intake Risks
Authors: Sun Ruiqing , Cao Junli , Li Jindong , Qi Yanli , Qin Shu
Published: October 2023

Fenpyroximate is an efficient, broad-spectrum phenoxypyrazole acaricide which is used for controlling various mites. In this study, we measured the levels of terminal fenpyroximate residues in citrus fruits, and estimated the dietary intake risks posed by fenpyroximate. To this end, a QuEChERS analytical method was used in combination with ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) to determine the residual levels of fenpyroximate and its Z-isomer (Z-fenpyroximate) in citrus fruits collected from 12 fields under good agricultural practices (GAPs). The average recoveries of fenpyroximate in whole fruits and citrus flesh were 104–110% and 92–109%, respectively, with corresponding RSDs of 1–4% and 1–3%. The average recoveries of Z-fenpyroximate were 104–113% and 90–91%, respectively, with RSDs of 1–2% in both cases. Each limit of quantification (LOQ) was 0.01 mg kg−1. Fifteen days after application with 56 mg kg−1, the terminal residues of fenpyroximate in whole fruits and citrus flesh were <0.010–0.18 mg kg−1 and <0.010–0.063 mg kg−1, respectively; the corresponding values for total fenpyroximate (the sum of fenpyroximate and Z-fenpyroximate) were <0.020–0.19 and <0.020–0.053 mg kg−1. The levels of terminal fenpyroximate residues in citrus fruit were less than the maximum residue limits (MRLs) specified in all the existing international standards. In addition, the risk quotients RQc and RQa were both less than 100%, indicating that the long-term and short-term dietary intake risks posed to Chinese consumers by fenpyroximate in citrus fruit are both acceptable after a 15-day harvest interval.
Ecofriendly Synthesis of Magnetic Composites Loaded on Rice Husks for Acid Blue 25 Decontamination: Adsorption Kinetics, Thermodynamics, and Isotherms
Authors: Batool Fozia , Kanwal Samia , Kanwal Hafsa , Noreen Sobia , Hodhod Mohamed S. , Mustaqeem Muhammad , Sharif Gulnaz , Naeem Hafiza Komal , Zahid Javeria , Gaafar Abdel-Rhman Z.
Published: October 2023

Addressing the growing need for methods for ecofriendly dye removal from aqueous media, this study explores the potential of rice husks coated with iron oxide (Fe2O3@RH composites) for efficient Acid Blue 25 decontamination. The adsorption potential of Acid Blue 25 is analyzed using raw rice husks and Fe2O3 nanoparticles in the literature, but their enhanced removal capacity by means of Fe2O3@RH composites is reported for the first time in this study. Fe2O3@RH composites were analyzed by using analytical techniques such as TGA, SEM, FTIR, BET, and the point of zero charge (pH(PZC)). The Acid Blue 25 adsorption experiment using Fe2O3@RH composites showed maximum adsorption at an initial concentration of Acid Blue 25 of 80 ppm, a contact time of 50 min, a temperature of 313 K, 0.25 g of Fe2O3@RH composites, and a pH of 2. The maximum percentage removal of Acid Blue 25 was found to be 91%. Various linear and nonlinear kinetic and isothermal models were used in this study to emphasize the importance and necessity of the adsorption process. Adsorption isotherms such as the Freundlich, Temkin, Langmuir, and Dubinin–Radushkevich (D–R) models were applied. The results showed that all the isotherms were best fitted on the data, except the linear form of the D–R isotherm. Adsorption kinetics such as the intraparticle kinetic model, the Elovich kinetic model, and the pseudo-first-order and pseudo-second-order models were applied. All the kinetic models were found to be best fitted on the data, except the PSO model (types II, III, and IV). Thermodynamic parameters such as ΔG° (KJ/mol), ΔH° (KJ/mol), and ΔS° (J/K*mol) were studied, and the reaction was found to be exothermic in nature with an increase in the entropy of the system, which supported the adsorption phenomenon. The current study contributes to a comprehensive understanding of the adsorption process and its underlying mechanisms through characterization, the optimization of the conditions, and the application of various models. The findings of the present study suggest practical applications of this method in wastewater treatment and environmental remediation.
Development and Characterization of High-Absorption Microencapsulated Organic Propolis EPP-AF® Extract (i-CAPs)
Authors: Berretta Andresa A. , De Lima Jéssica A. , Falcão Soraia I. , Calhelha Ricardo , Amorim Nathaly Alcazar , Gonçalves Isabella Salgado , Zamarrenho Luana Gonçalves , Barud Hernane da Silva , Bastos Jairo Kenupp , De Jong David , Vilas-Boas Miguel
Published: October 2023

The demand for organic and functional food continues to increase yearly. Among the available functional foods, propolis is a bee product that has various beneficial properties, including antimicrobial, antioxidant, and anti-inflammatory activities. However, it generally is only available in ethanol solution, which has poor bioavailability, as it is relatively insoluble in water. The use of such ethanol extracts is often objectionable because of the alcohol content and because they have a strong and striking taste. Development of alternatives that can efficiently and safely increase solubility in water, and that meet organic production specifications, has been a challenge. To address these concerns, microcapsules were developed using spray-dryer technology from an emulsion based on EPP-AF® propolis and gum arabic (i-CAPS). These propolis-loaded microcapsules were characterized using FT-IR, SEM, TGA, HPLC, and spectrophotometric techniques, along with determination of antimicrobial, antioxidant, antitumor, anti-inflammatory, and antihypercholesterolemic activities, as well as permeability in in vitro models. The production system resulted in microcapsules with a spherical shape and an encapsulation efficiency of 93.7 ± 0.7%. They had IC50s of 2.654 ± 0.062 and 7.342 ± 0.058 µg/mL by FRAP and DPPH antioxidant methods, respectively. The EPP-AF® i-CAPS also had superior antimicrobial activity against Gram-positive bacteria. Antitumor activity was calculated based on the concentration that inhibited 50% of growth of AGS, Caco-2, and MCF-7 cell strains, giving results of 154.0 ± 1.0, 117 ± 1.0, and 271.0 ± 25 µg/mL, respectively. The microcapsule presentation reduced the permeation of cholesterol by 53.7%, demonstrating antihypercholesterolemic activity, and it improved the permeability of p-coumaric acid and artepillin C. The IC50 for NO production in RAW 264.7 cells was 59.0 ± 0.1 µg/mL. These findings demonstrate the potential of this new propolis product as a food and pharmaceutical ingredient, though additional studies are recommended to validate the safety of proposed dosages.
The Adsorption Mechanisms of SF6-Decomposed Species on Tc- and Ru-Embedded Phthalocyanine Surfaces: A Density Functional Theory Study
Authors: Xue Rou , Jiang Wen , He Xing , Xiong Huihui , Xie Gang , Nie Zhifeng
Published: October 2023

Designing high-performance materials for the detection or removal of toxic decomposition gases of sulfur hexafluoride is crucial for both environmental monitoring and human health preservation. Based on first-principles calculations, the adsorption performance and gas-sensing properties of unsubstituted phthalocyanine (H2Pc) and H2Pc doped with 4d transition metal atoms (TM = Tc and Ru) towards five characteristic decomposition components (HF, H2S, SO2, SOF2, and SO2F2) were simulated. The findings indicate that both the TcPc and RuPc monolayers are thermodynamically and dynamically stable. The analysis of the adsorption energy indicates that H2S, SO2, SOF2, and SO2F2 underwent chemisorption on the TcPc monolayer. Conversely, the HF molecules were physisorbed through interactions with H atoms. The chemical adsorption of H2S, SO2, and SOF2 occurred on the RuPc monolayer, while the physical adsorption of HF and SO2F2 molecules was observed. Moreover, the microcosmic mechanism of the gas–adsorbent interaction was elucidated by analyzing the charge density differences, electron density distributions, Hirshfeld charges, and density of states. The TcPc and RuPc monolayers exhibited excellent sensitivity towards H2S, SO2, and SOF2, as evidenced by the substantial alterations in the band gaps and work functions of the TcPc and RuPc nanosheets. Our calculations hold significant value for exploring the potential chemical sensing applications of TcPc and RuPc monolayers in gas sensing, with a specific focus on detecting sulfur hexafluoride.
Hexa-Histidine, a Peptide with Versatile Applications in the Study of Amyloid-β(1–42) Molecular Mechanisms of Action
Authors: Salazar Jairo , Samhan-Arias Alejandro K. , Gutierrez-Merino Carlos
Published: October 2023

Amyloid β (Aβ) oligomers are the most neurotoxic forms of Aβ, and Aβ(1–42) is the prevalent Aβ peptide found in the amyloid plaques of Alzheimer’s disease patients. Aβ(25–35) is the shortest peptide that retains the toxicity of Aβ(1–42). Aβ oligomers bind to calmodulin (CaM) and calbindin-D28k with dissociation constants in the nanomolar Aβ(1–42) concentration range. Aβ and histidine-rich proteins have a high affinity for transition metal ions Cu2+, Fe3+ and Zn2+. In this work, we show that the fluorescence of Aβ(1–42) HiLyteTM-Fluor555 can be used to monitor hexa-histidine peptide (His6) interaction with Aβ(1–42). The formation of His6/Aβ(1–42) complexes is also supported by docking results yielded by the MDockPeP Server. Also, we found that micromolar concentrations of His6 block the increase in the fluorescence of Aβ(1–42) HiLyteTM-Fluor555 produced by its interaction with the proteins CaM and calbindin-D28k. In addition, we found that the His6-tag provides a high-affinity site for the binding of Aβ(1–42) and Aβ(25–35) peptides to the human recombinant cytochrome b5 reductase, and sensitizes this enzyme to inhibition by these peptides. In conclusion, our results suggest that a His6-tag could provide a valuable new tool to experimentally direct the action of neurotoxic Aβ peptides toward selected cellular targets.
A Universal Volumetric Haptic Actuation Platform
Authors: Coe Patrick , Evreinov Grigori , Ziat Mounia , Raisamo Roope
Published: October 2023

In this paper, we report a method of implementing a universal volumetric haptic actuation platform which can be adapted to fit a wide variety of visual displays with flat surfaces. This platform aims to enable the simulation of the 3D features of input interfaces. This goal is achieved using four readily available stepper motors in a diagonal cross configuration with which we can quickly change the position of a surface in a manner that can render these volumetric features. In our research, we use a Microsoft Surface Go tablet placed on the haptic enhancement actuation platform to replicate the exploratory features of virtual keyboard keycaps displayed on the touchscreen. We ask seven participants to explore the surface of a virtual keypad comprised of 12 keycaps. As a second task, random key positions are announced one at a time, which the participant is expected to locate. These experiments are used to understand how and with what fidelity the volumetric feedback could improve performance (detection time, track length, and error rate) of detecting the specific keycaps location with haptic feedback and in the absence of visual feedback. Participants complete the tasks with great success (p < 0.05). In addition, their ability to feel convex keycaps is confirmed within the subjective comments.
Whey Protein Hydrolysate Exerts Anti-Inflammatory Effects to Alleviate Dextran Sodium Sulfate (DSS)-Induced Colitis via Microbiome Restoration
Authors: Zou Wenrong , Fu Zixin , Guo Xiaohong , Yao Lei , Hong Hui , Luo Yongkang , Tan Yuqing
Published: October 2023

Whey protein hydrolysate (WPH) has been shown to have a variety of bioactivities. This study aimed to investigate the preventive effect of WPH on dextran sodium sulfate (DSS)-induced colitis in C57BL/6J mice. The results indicated that WPH intervention for 37 days was effective in delaying the development of colonic inflammation, and high doses of WPH significantly inhibited weight loss (9.16%, n = 8, p < 0.05), protected the colonic mucosal layer, and significantly reduced the levels of inflammatory factors TNF-α, IL-6, and IL-1β in mice with colitis (n = 8, p < 0.05). In addition, WPH intervention was able to up-regulate the short-chain fatty acids secretion and restore the gut microbiome imbalance in mice with colitis. Notably, high-dose WPH intervention increased the relative abundance of norank_f_Muribaculaceae by 1.52-fold and decreased the relative abundance of Romboutsia and Enterobacter by 3.77-fold and 2.45-fold, respectively, compared with the Model group. WPH intervention protected colitis mice mainly by reversing the microbiome imbalance and regulating the major histocompatibility complex (MHC) class I pathway. This study showed that WPH has anti-inflammatory activity and a promising colitis management future.
Tributyrin Mitigates Ethanol-Induced Lysine Acetylation of Histone-H3 and p65-NFκB Downregulating CCL2 Expression and Consequent Liver Inflammation and Injury
Authors: Ghare Smita S. , Charpentier Benjamin T. , Ghooray Dushan T. , Zhang Jingwen , Vadhanam Manicka V. , Reddy Sreelatha , Joshi-Barve Swati , McClain Craig J. , Barve Shirish S.
Published: October 2023

Purpose: Chemokine-driven leukocyte infiltration and sustained inflammation contribute to alcohol-associated liver disease (ALD). Elevated hepatic CCL2 expression, seen in ALD, is associated with disease severity. However, mechanisms of CCL2 regulation are not completely elucidated. Post-translational modifications (PTMs) of proteins, particularly acetylation, modulate gene expression. This study examined the acetylation changes of promoter-associated histone-H3 and key transcription factor-NFκB in regulating hepatic CCL2 expression and subsequent inflammation and injury. Further, the effect of therapeutic modulation of the acetylation state by tributyrin (TB), a butyrate prodrug, was assessed. Methods: Hepatic CCL2 expression was assessed in mice fed control (PF) or an ethanol-containing Lieber–DeCarli (5% v/v, EF) diet for 7 weeks with or without oral administration of tributyrin (TB, 2 g/kg, 5 days/week). A chromatin immunoprecipitation (ChIP) assay evaluated promoter-associated modifications. Nuclear association between SIRT1, p300, and NFκB-p65 and acetylation changes of p65 were determined using immunoprecipitation and Western blot analyses. A Student’s t-test and one-way ANOVA determined the significance. Results: Ethanol significantly increased promoter-associated histone-H3-lysine-9 acetylation (H3K9Ac), reflecting a transcriptionally permissive state with a resultant increase in hepatic CCL2 mRNA and protein expression. Moreover, increased lysine-310-acetylation of nuclear RelA/p65 decreased its association with SIRT1, a class III HDAC, but concomitantly increased with p300, a histone acetyltransferase. This further led to enhanced recruitment of NF-κB/p65 and RNA polymerase-II to the CCL2 promoter. Oral TB administration prevented ethanol-associated acetylation changes, thus downregulating CCL2 expression, hepatic neutrophil infiltration, and inflammation/ injury. Conclusion: The modulation of a protein acetylation state via ethanol or TB mechanistically regulates hepatic CCL2 upregulation in ALD.
Evolution and Current Status of Influenza A Virus in Chile: A Review
Authors: Godoy Marcos , Oca Marco Montes de , Caro Diego , Pontigo Juan Pablo , Kibenge Molly , Kibenge Frederick
Published: October 2023

The influenza A virus (IAV) poses a significant global threat to public health and food security. Particularly concerning is the avian influenza virus (AIV) subtype H5N1, which has spread from Europe to North and Central/South America. This review presents recent developments in IAV evolution in birds, mammals, and humans in Chile. Chile’s encounter with IAV began in 2002, with the highly pathogenic avian influenza (HPAI) H7N3 virus, derived from a unique South American low pathogenic avian influenza (LPAI) virus. In 2016–2017, LPAI H7N6 caused outbreaks in turkey, linked to wild birds in Chile and Bolivia. The pandemic influenza A (H1N1) 2009 (H1N1pdm09) virus in 2009 decreased egg production in turkeys. Since 2012, diverse IAV subtypes have emerged in backyard poultry and pigs. Reassortant AIVs, incorporating genes from both North and South American isolates, have been found in wild birds since 2007. Notably, from December 2022, HPAI H5N1 was detected in wild birds, sea lions, and a human, along Chile’s north coast. It was introduced through Atlantic migratory flyways from North America. These findings emphasize the need for enhanced biosecurity on poultry farms and ongoing genomic surveillance to understand and manage AIVs in both wild and domestic bird populations in Chile.
Phytochemical Investigation of Equisetum arvense and Evaluation of Their Anti-Inflammatory Potential in TNFα/INFγ-Stimulated Keratinocytes
Authors: Jeong Se Yun , Yu Hyung-Seok , Ra Moon-Jin , Jung Sang-Mi , Yu Jeong-Nam , Kim Jin-Chul , Kim Ki Hyun
Published: October 2023

Equisetum arvense L. (Equisetaceae), widely known as ‘horsetail’, is a perennial plant found extensively across Asia. Extracts of E. arvense have been used in traditional medicine, particularly for the treatment of inflammatory disorders. This study aimed to determine the phytochemical compounds in E. arvense ethanolic extract and their anti-inflammatory properties. Subsequently, we isolated and identified nine secondary metabolites, including kaempferol 3,7-di-O-β-D-glucopyranoside (1), icariside B2 (2), (Z)-3-hexenyl β-D-glucopyranoside (3), luteolin 5-O-β-D-glucopyranoside (4), 4-O-β-D-glucopyranosyl caffeic acid (5), clemastanin B (6), 4-O-caffeoylshikimic acid (7), (7S,8S)-threo-7,9,9′-trihydroxy-3,3′-dimethoxy-8-O-4′-neolignan-4-O-β-D-glucopyranoside (8), and 3-O-caffeoylshikimic acid (9). The chemical structures of the isolated compounds (1–9) were elucidated using HR-ESI-MS data, NMR spectra, and ECD data. Next, the anti-inflammatory effects of the isolates were evaluated in tumor necrosis factor (TNF)α/interferon (IFN)γ-induced HaCaT, a human keratinocyte cell line. Among the isolates, compound 3 showed the highest inhibitory effect on the expression of pro-inflammatory chemokines, followed by compounds 6 and 8. Correspondingly, the preceding isolates inhibited TNFα/IFNγ-induced activation of pro-inflammatory transcription factors, signal transducer and activator of transcription 1, and nuclear factor-κB. Collectively, E. arvense could be employed for the development of prophylactic or therapeutic agents for improving dermatitis.
Anti-Viral Activity of Bioactive Molecules of Silymarin against COVID-19 via In Silico Studies
Authors: Zhang Chunye , Sui Yuxiang , Liu Shuai , Yang Ming
Published: October 2023

The severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) infection drove the global coronavirus disease 2019 (COVID-19) pandemic, causing a huge loss of human life and a negative impact on economic development. It is an urgent necessity to explore potential drugs against viruses, such as SARS-CoV-2. Silymarin, a mixture of herb-derived polyphenolic flavonoids extracted from the milk thistle, possesses potent antioxidative, anti-apoptotic, and anti-inflammatory properties. Accumulating research studies have demonstrated the killing activity of silymarin against viruses, such as dengue virus, chikungunya virus, and hepatitis C virus. However, the anti-COVID-19 mechanisms of silymarin remain unclear. In this study, multiple disciplinary approaches and methodologies were applied to evaluate the potential mechanisms of silymarin as an anti-viral agent against SARS-CoV-2 infection. In silico approaches such as molecular docking, network pharmacology, and bioinformatic methods were incorporated to assess the ligand–protein binding properties and analyze the protein–protein interaction network. The DAVID database was used to analyze gene functions, such as the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) enrichment. TCMSP and GeneCards were used to identify drug target genes and COVID-19-related genes. Our results revealed that silymarin compounds, such as silybin A/B and silymonin, displayed triplicate functions against SARS-CoV-2 infection, including directly binding with human angiotensin-converting enzyme 2 (ACE2) to inhibit SARS-CoV-2 entry into the host cells, directly binding with viral proteins RdRp and helicase to inhibit viral replication and proliferation, and regulating host immune response to indirectly inhibit viral infection. Specifically, the targets of silymarin molecules in immune regulation were screened out, such as proinflammatory cytokines TNF and IL-6 and cell growth factors VEGFA and EGF. In addition, the molecular mechanism of drug-target protein interaction was investigated, including the binding pockets of drug molecules in human ACE2 and viral proteins, the formation of hydrogen bonds, hydrophobic interactions, and other drug–protein ligand interactions. Finally, the drug-likeness results of candidate molecules passed the criteria for drug screening. Overall, this study demonstrates the molecular mechanism of silymarin molecules against SARS-CoV-2 infection.
Wavelength-Tunable Pulsed Cylindrical Vector Beams in a 1.7-μm Mode-Locking Thulium-Doped All-Fiber Laser
Authors: Yang Xiaoliang , Long Rufei , Xie Yuhua , Wen Jiahao , Lv Hongwei , Chen Yu
Published: October 2023

Because of the special absorption peak, pulsed lasers at 1.7 μm have been rapidly developed in medical treatment, biological imaging and so on. Introducing the cylindrical vector beam (CVB) may further promote these special applications due to its unique intensity, phase and polarization characteristics. Herein, we have experimentally demonstrated the generation of wavelength-tunable pulsed CVBs at 1.7 μm based on a thulium-doped all-fiber laser. A bandpass filter with a wide bandwidth combined with nonlinear polarization rotation technology is used to obtain pulsed laser emission at 1.7 μm. By taking advantage of a home-made Lyot filter and mode selective coupler (MSC), pulsed CVBs can be obtained with a wavelength tuning range of 66 nm (1720–1786 nm). The development of wavelength-tunable pulsed CVBs at the 1.7 μm waveband has significant potential applications in deep bioimaging and laser processing.
Bacterial Population Changes during the Degradation Process of a Lactate (LA)-Enriched Biodegradable Polymer in River Water: LA-Cluster Preferable Bacterial Consortium
Authors: Kadoya Ryosuke , Soga Hitomi , Matsuda Miki , Sato Michio , Taguchi Seiichi
Published: October 2023

The lactate-based polyester poly[lactate (LA)-co-3-hydroxybutyrate (3HB)], termed LAHB, is a highly transparent and flexible bio-based polymeric material. There are many unknowns regarding its degradation process in riverine environments, especially the changes in bacterial flora that might result from its degradation and the identities of any LAHB-degrading bacteria. LAHB were immersed in the river water samples (A and B), and LAHB degradation was observed in terms of the weight change of the polymer and the microscopic changes on the polymer surfaces. A metagenomic analysis of microorganisms was conducted to determine the effect of LAHB degradation on the aquatic environment. The bacterial flora obtained from beta diversity analysis differed between the two river samples. The river A water sample showed the simultaneous degradation of LA and 3HB even though the copolymer was LA-enriched, suggesting preferable hydrolysis of the LA-enriched segments. In contrast, only 3HB degraded for the LAHB in the river B water sample. The linear discriminant analysis effect size (LEfSe) analysis revealed 14 bacteria that were significantly increased in the river A water sample during LAHB degradation, suggesting that these bacteria preferentially degraded and assimilated LA-clustering polymers. Our metagenomic analysis provides useful insights into the dynamic changes in microbial communities and LA-clustering polymer-degrading bacteria.