bluuebunny/crossref_metadata_2025 huggingface dataset:
Exocrine and Endocrine Insufficiency in Autoimmune Pancreatitis: A Matter of Treatment or Time?
Authors: Nikolic Sara , Maisonneuve Patrick , Dahlman Ingrid , Löhr J.-Matthias , Vujasinovic Miroslav
Published: June 2022

Background: Autoimmune pancreatitis (AIP) is a specific form of chronic pancreatitis with a high relapse rate after treatment. AIP patients are burdened with an increased risk of long-term sequelae such as exocrine and endocrine insufficiency. Our objective was to investigate if pharmacological treatment affects both endocrine and exocrine pancreatic function in patients with AIP. Methods: We included 59 patients with definite AIP in the final analysis. Screening for diabetes mellitus (DM) and pancreatic exocrine insufficiency (PEI) was performed at the time of AIP diagnosis and during follow-up. Results: There were 40 (67.8%) males and 19 (32.2%) females; median age at diagnosis was 65 years. Median follow-up after the diagnosis of AIP was 62 months. PEI prevalence at diagnosis was 72.7% and was 63.5% at follow-up. The cumulative incidence of DM was 17.9%, with a prevalence of DM at diagnosis of 32.8%. No strong association was found between pharmacological treatment and occurrence of PEI and DM. Univariate analysis identified potential risk factors for PEI (other organ involvement and biliary stenting) and for DM (overweight, blue-collar profession, smoking, weight loss or obstructive jaundice as presenting symptoms, imaging showing diffuse pancreatic enlargement, smoking). In a multivariate analysis, only obstructive jaundice was identified as a risk factor for DM both at diagnosis and during follow-up. Conclusions: Our results suggest that the prevalence of endocrine and exocrine insufficiency in AIP is high at diagnosis with an additional risk of PEI and DM during follow-up despite pharmacological treatment.
Accurate Fish Detection under Marine Background Noise Based on the Retinex Enhancement Algorithm and CNN
Authors: Chen Yanhu , Ling Yucheng , Zhang Luning
Published: June 2022

Underwater detection equipment with fish detection technology has broad application prospects in marine fishery resources exploration and conservation. In this paper, we establish a multi-scale retinex enhancement algorithm and a multi-scale feature-based fish detection model to improve underwater detection accuracy and ensure real-time performance. During image preprocessing, the enhancement algorithm combines the bionic structure of the fish retina and classical retinex theory to filter out underwater environmental noise. The detection model focuses on improving the detection performance on small-size targets using a deep learning method based on a convolutional neural network. We compare our method to current mainstream detection models (Faster R-CNN, RetinaNet, YOLO, SSDetc.), and the proposed model achieves better performance, with a mean Average Precision (mAP) of 78.31% and a mean Miss Rate (mMR) of 54.11% in the open fish image data set. The test results for the data from the field experiment prove the feasibility and stability of our model.
On the Turbulent Drag Reduction Effect of the Dynamic Free-Slip Surface Method
Authors: Wang Cong , Gharib Morteza
Published: June 2022

The turbulent boundary layer (TBL) over the hull surface of a water vehicle significantly elevates the drag force on the water vehicle. In this regard, effectively controlling the TBL can lead to a drag reduction (DR) effect and therefore improve the energy efficiency of water transportation. Many DR methods have demonstrated promising DR effects but face challenges in implementation at the scale of engineering application. In this regard, the recently developed dynamic free-slip surface method can resolve some of the critical challenges. It employs an array of freely oscillating air–water interfaces to manipulate the TBL and can achieve a substantial DR effect under certain control conditions. However, the optimal setting of the control parameters that would maximize the DR effect remains unclear. To answer these questions, this study systematically investigates the effects of multiple control parameters for the first time, including the geometric size and curvature of the interface, the frequency of active oscillation, and the Reynolds number of TBL. Digital Particle Image Velocimetry was used to non-invasively measure the velocity and vorticity field of the TBL, and the Charted Clauser method was used to calculate the DR effect. The presented results suggest that the oscillating free-slip interfaces reduce the flow velocity near the wall boundary and lift the transverse vorticity (and the viscous shear stress) away from the wall. In addition, the shape factor of the TBL is elevated by the oscillating interfaces and slowly relaxes back in the downstream regions, which implies a partial relaminarization process induced in the TBL. Up to 36% DR effect was achieved within the current scope range of the control parameters. All of the results consistently suggest that a large DR effect is achieved when the free-slip interfaces oscillate with large Weber numbers. These discoveries shed light on the underlying DR mechanism and provide guidance for the future development of an effective drag control technique based on the dynamic free-slip surface method.
Late Quaternary Evolution of a Submerged Karst Basin Influenced by Active Tectonics (Koločep Bay, Croatia)
Authors: Šolaja Dragana , Miko Slobodan , Brunović Dea , Ilijanić Nikolina , Hasan Ozren , Papatheodorou George , Geraga Maria , Durn Tatjana , Christodoulou Dimitris , Razum Ivan
Published: June 2022

Koločep bay is a 30 km elongated karst basin located between the Elafiti Islands and the mainland with a NW–SE general direction. The bay lies within the seismically active southern Dalmatia zone. Irregular grid sub-bottom profiles and two legacy reflection seismic profiles have been used to determine the overall morphology of the bay and to establish the seismic stratigraphy of its sedimentary infill. Three major seismic–stratigraphic units have been identified in the upper ~50 m of the ~120-meter-thick sedimentary sequence that lies above the karstified limestone bedrock. The karst polje basin was flooded due to sea-level rise before 12.1 kyr BP. The morphology of the bay implies complex influences of Late Glacial meltwater discharges, aeolian sand deposition, the existence of paleo–ponor/karst spring zones and buried Pleistocene river channels. The Pleistocene seismotectonic units are deformed in the NW and SE parts of the basin. The central part of the basin has no signatures of intensive tectonic activity during the Holocene. A major erosion event was identified that led to the formation of a basin within the older sedimentary infill. In the southern part of the basin, we have evidence of Holocene tectonic activity with the formation of erosional scarps on the seafloor of the bay.
Study on the Pressure–Sinkage Process and Constitutive Model of Deep-Sea Sediment
Authors: Wei Dingbang , Cao Huade , Xia Jianxin
Published: June 2022

The driving performance of subsea mining vehicles is greatly affected by the pressure–sinkage characteristics of deep-sea sediment. Therefore, it is of great importance to analyze the microscopic properties of deep-sea sediment and establish the corresponding pressure–sinkage model for the safe operation of subsea mining vehicles. Hence, the present paper focuses on the physical properties of deep-sea sediment to provide a preliminary understanding of its pressure–sinkage process and evolution according to the solid–liquid two-phase flow characteristics and particle flow mechanism. In addition, the stress loading time and the rheological theory are applied in order to introduce a four-element model that describes the various pressure–sinkage stages that correspond to each stage of deep-sea sediment evolution. On this basis, the parameters of the pressure–sinkage constitutive model are determined by a specific calculation method. Moreover, a new pressure–sinkage constitutive model of deep-sea sediment that considers the time-variable mechanical properties is established in order to describe the full sinkage process. Finally, research results from the existing literature and experimental data are used to verify the rationality and correctness of the model. The results show that the proposed pressure–sinkage constitutive model is in good agreement with experimental data and is effective in describing the evolution of the mechanical properties and the trend in the sinkage rate of deep-sea sediment at various stages. A comparison with the Kelvin model indicates that the proposed pressure–sinkage constitutive model provides superior accuracy with the use of fewer parameters. Consequently, this study can provide a theoretical basis and technical support for the design of subsea mining vehicles.
Seasonal Variation of Catenary-Bead Dunes in the Yangtze River Estuary: Causes and Implications
Authors: Zheng Shuwei , Yuan Xinmeng , Yang Suxian , Cheng Heqin , Guo Xingjie , Cui Yurong , Liu Enfeng , Li Zijun
Published: June 2022

Low-angle lee-side slopes of dunes are commonly developed on the world’s riverbeds, and dune migration associated with sediment transport exert a major influence on riverine processes. However, the catenary-bead dune has been identified in the Yangtze River (YR) Estuary, featuring a higher lee-side angle. To date, the morphological variation and formation reasons of catenary-bead dunes in the YR Estuary remain uncharacterized. In this study, we used a multibeam echo system (MBES) to investigate the bedforms of the YR estuary during 2014–2015, as well as to discuss the seasonal variation of catenary-bead dunes. The results indicate that the catenary-bead dunes of the YR Estuary are characterized by growth during the flood season and extinction during the dry season. The lee-side angle is typically ~16.7°, which is larger than that of other dune types (3.7–8°) in the estuary; moreover, the catenary-bead dunes are higher than other dune types of the same length in the YR Estuary. The relationship between the dune height (H) and length (L) was found to be H = 0.1667L0.603 (R2 = 0.38), while the other dune types yielded the relationship of H = 0.0845L0.758 (R2 = 0.52). Strong runoff superimposing the ebb tide led to the development of catenary-bead dunes. Furthermore, the higher coarse sediment content (69.9–72%) and lower clay content (6.3–6.7%) of the riverbed sediment are favorable for their formation, while the higher curved crest-lines are favorable for the formation of the associated elliptical pits.
In-Situ Estimates of Net Ecosystem Metabolisms in the Rocky Habitats of Dokdo Islets in the East Sea of Korea
Authors: Lee Jae Seong , Kim Sung-Han , Min Won-Gi , Choi Dong Mun , Lee Eun Kyung , Kim Kyung-Tae , An Sung-Uk , Baek Ju-Wook , Lee Won-Chan , Park Chan Hong
Published: June 2022

We measured oxygen (O2) fluxes in two major shallow subtidal benthic habitats (kelp bed (KB) and bare rock (BR) covered with crustose coralline algae) of Dokdo islet in the East Sea by applying noninvasive in-situ aquatic eddy covariance (AEC). The AEC device allows time series measurements (~24 h) of three-dimensional velocity (u, v, and w components) and high-resolution dissolved O2. This allows estimation of O2 exchange flux via benthic habitats. Local flow rates and irradiance levels were found to be major factors controlling O2 exchange flux in the rocky habitats. Gross primary production rates tended to be significantly higher in KB (163 mmol O2 m−2 d−1) than in BR (51 mmol O2 m−2 d−1). The net ecosystem metabolisms were assessed as opposite types, with 8 mmol O2 m−2 d−1 in KB (autotrophy) and –12 mmol O2 m−2 d−1 in BR (heterotrophy). Our results indicate that kelp beds are important for organic carbon cycling in rocky coastal waters and that AEC application to macroalgae habitats is a useful assessment approach.
Factors Controlling Dead Wood Decomposition in an Old-Growth Temperate Forest in Central Europe
Authors: Jomura Mayuko , Yoshida Riki , Michalčíková Lenka , Tláskal Vojtěch , Baldrian Petr
Published: June 2022

Dead wood represents an important pool of carbon and nitrogen in forest ecosystems. This source of soil organic matter has diverse ecosystem functions that include, among others, carbon and nitrogen cycling. However, information is limited on how deadwood properties such as chemical composition, decomposer abundance, community composition, and age correlate and affect decomposition rate. Here, we targeted coarse dead wood of beech, spruce, and fir, namely snags and tree trunks (logs) in an old-growth temperate forest in central Europe; measured their decomposition rate as CO2 production in situ; and analyzed their relationships with other measured variables. Respiration rate of dead wood showed strong positive correlation with acid phosphatase activity and negative correlation with lignin content. Fungal biomass (ergosterol content) and moisture content were additional predictors. Our results indicate that dead wood traits, including tree species, age, and position (downed/standing), affected dead wood chemical properties, microbial biomass, moisture condition, and enzyme activity through changes in fungal communities and ultimately influenced the decomposition rate of dead wood.
Isavuconazole for COVID-19-Associated Invasive Mold Infections
Authors: Ullah Nadir , Sepulcri Chiara , Mikulska Malgorzata
Published: June 2022

Isavuconazole is a broad-spectrum antifungal drug recently approved as a first-line treatment for invasive aspergillosis and as a first or alternative treatment for mucormycosis. The purpose of this review was to report and discuss the use of isavuconazole for the treatment of COVID-19-associated aspergillosis (CAPA), and COVID-19-associated mucormycosis (CAM). Among all studies which reported treatment of CAPA, approximately 10% of patients were reportedly treated with isavuconazole. Considering 14 identified studies that reported the use of isavuconazole for CAPA, isavuconazole was used in 40% of patients (95 of 235 treated patients), being first-line monotherapy in over half of them. We identified six studies that reported isavuconazole use in CAM, either alone or in combination therapy. Overall, isavuconazole was used as therapy in 13% of treated CAM patients, frequently as combination or sequential therapy. The use of isavuconazole in CAPA and CAM is complicated by the challenge of achieving adequate exposure in COVID-19 patients who are frequently obese and hospitalized in the ICU with concomitant renal replacement therapy (RRT) or extracorporeal membrane oxygenation (ECMO). The presence of data on high efficacy in the treatment of aspergillosis, lower potential for drug–drug interactions (DDIs) and for subtherapeutic levels, and no risk of QT prolongation compared to other mold-active azoles, better safety profile than voriconazole, and the possibility of using an intravenous formulation in the case of renal failure are the advantages of using isavuconazole in this setting.
Towards a Fungal Science That Is Independent of Researchers’ Gender
Authors: Kraševec Nada
Published: June 2022

The main drivers of gender mainstreaming in basic and clinical research appear to be funding agencies and scientific journals. Some funding agencies have already recognized the importance of their actions for the global development of ideas in science, but further targeted efforts are needed. The challenges for women scientists in fungal research appear to be similar to those in other science, technology, engineering, and mathematics disciplines, although the gender gap in mycology publishing appears to be less pronounced; however, women are underrepresented as last (corresponding) authors. Two examples of best practices to bridge the gap have been promoted in the fungal community: “power hour” and a central resource database for women researchers of fungi and oomycetes. A more balanced ratio of women researchers among (plenary) session speakers, (plenary) session chairs, and committee members at the recent fungal genetics conference is an encouraging sign that the gender gap can be closed. The editorial policy of some journals follows the guidance “Sex and Gender Equality in Research,” and other journals should follow, and indicate the gender ratio among authors and reviewers.
A Dramaturgical Analysis of Latina Influencers Use of Props and Settings to Signal Identity
Authors: Soto-Vásquez Arthur D. , Jimenez Nadia
Published: June 2022

Dramaturgical analysis has been applied by scholars to social media influencers, but how props and settings are used to signal identity is understudied. This study uses a series of in-depth interviews with Latina influencers who live and work in a mid-size city on the U.S./Mexico border and an analysis of corresponding posts to explore how props and settings can be used to signal gender and race while also communicating authenticity. The findings show that influencers have to carefully and strategically navigate the use of props and settings not to appear fake and contrived. They blend the use of frontstage props with calibrated sharing of backstage settings to approximate an authentic online performance of their branded identity that is approachable but also monetizable. When performing their gender, the influencers adopt a having-it-all performance, balancing family, beauty, career success, and health while using backstage settings to create connection. Finally, Latina influencers on the border portray it as a setting that differs from its mainstream representation as a place to avoid. They also strategically deploy Latina identity to market themselves and localize national trends.
Research Progress of the Endocrine-Disrupting Effects of Disinfection Byproducts
Authors: Sui Shuxin , Liu Huihui , Yang Xianhai
Published: June 2022

Since 1974, more than 800 disinfection byproducts (DBPs) have been identified from disinfected drinking water, swimming pool water, wastewaters, etc. Some DBPs are recognized as contaminants of high environmental concern because they may induce many detrimental health (e.g., cancer, cytotoxicity, and genotoxicity) and/or ecological (e.g., acute toxicity and development toxicity on alga, crustacean, and fish) effects. However, the information on whether DBPs may elicit potential endocrine-disrupting effects in human and wildlife is scarce. It is the major objective of this paper to summarize the reported potential endocrine-disrupting effects of the identified DBPs in the view of adverse outcome pathways (AOPs). In this regard, we introduce the potential molecular initiating events (MIEs), key events (KEs), and adverse outcomes (AOs) associated with exposure to specific DBPs. The present evidence indicates that the endocrine system of organism can be perturbed by certain DBPs through some MIEs, including hormone receptor-mediated mechanisms and non-receptor-mediated mechanisms (e.g., hormone transport protein). Lastly, the gaps in our knowledge of the endocrine-disrupting effects of DBPs are highlighted, and critical directions for future studies are proposed.
Determinants of Left Atrial Compliance in the Metabolic Syndrome: Insights from the “Linosa Study”
Authors: Barbier Paolo , Palazzo Adriano Edvige , Lucini Daniela , Pagani Massimo , Cusumano Gaspare , De Maria Beatrice , Dalla Vecchia Laura Adelaide
Published: June 2022

The association between left atrial (LA) impairment and cardiovascular diseases (CVD) and between dyslipidaemia and CVD are well known. The present study aims to investigate the relationships between metabolic factors and LA dimensions and compliance, as well as test the hypothesis that metabolic factors influence LA function independent from hemodynamic mechanisms. Arterial blood pressure (BP), waist and hip circumference, metabolic indices, and a complete echocardiographic assessment were obtained from 148 selected inhabitants (M/F 89/59; age 20–86 years) of Linosa Island, who had no history of CVD. At enrollment, 27.7% of the subjects met the criteria for metabolic syndrome (MetS) and 15.5% for arterial hypertension (HTN). LA compliance was reduced in subjects with MetS compared to those without (53 ± 27% vs. 71 ± 29%, p = 0.04) and was even lower (32 ± 17%, p = 0.01) in those with MetS and HTN. At multiple regression analysis, the presence of MetS independently determined LA maximal area (r = 0.56, p < 0.001), whereas systolic BP and the total cholesterol/HDL cholesterol ratio determined LA compliance (r = 0.41, p < 0.001). In an apparently healthy population with a high prevalence of MetS, dyslipidaemia seems to independently influence LA compliance. At a 5-year follow-up, LA compliance was reduced in both all-cause and CVD mortality groups, and markedly impaired in those who died of CVD. These findings may contribute to understanding the prognostic role of LA function in CVD and strengthen the need for early and accurate lipid control strategies.
The Correlation of Carpal Tunnel Pressure with Clinical Outcomes following Ultrasonographically-Guided Percutaneous Carpal Tunnel Release
Authors: Wang Jui-Chien , Li Chung-Yi , Ko Po-Yen , Wu Tung-Tai , Wu Kuo-Chen , Su Fong-Chin , Jou I-Ming , Wu Po-Ting
Published: June 2022

Background: To evaluate the correlation between carpal tunnel pressure (CTP) and the clinical presentations, and to explore the possible predictors for the postoperative recovery pattern in patients with carpal tunnel syndrome (CTS). Materials and Methods: Consecutive patients with idiopathic CTS following percutaneous ultrasound-guided carpal tunnel release (UCTR) were enrolled. CTP was measured preoperatively and immediately after operation. The Boston Carpal Tunnel Questionnaire (BCTQ) and the cross-sectional area (CSA) of median nerve were recorded preoperatively and at 1, 3, and 12 months postoperatively. Results: 37 patients (37 hands; 8 men and 29 females; median age, 59.0 years) were enrolled. CTP significantly decreased immediately from 40.0 (28.0–58.0) to 13.0 (8.0–20.0) mmHg after UCTR. BCTQ scores significantly improved at 1 month postoperatively, and the improvement trend persisted until 12 months postoperatively (p < 0.001). Preoperative CTP was positively correlated with preoperative CSA and preoperative BCTQ scores (p < 0.05, all). Using group-based trajectory modeling, all patients were categorized into the “gradual recovery” or “fast recovery” group. Higher preoperative CTP was significantly associated with a faster recovery pattern (odds ratio: 1.32). Conclusions: Preoperative CTP was well correlated with the clinical presentations and might be a useful predictor for the postoperative clinical recovery pattern.
Predicting Remission among Perinatal Women with Depression in Rural Pakistan: A Prognostic Model for Task-Shared Interventions in Primary Care Settings
Authors: Waqas Ahmed , Sikander Siham , Malik Abid , Atif Najia , Karyotaki Eirini , Rahman Atif
Published: June 2022

Perinatal depression is highly prevalent in low- and middle-income countries (LMICs) and is associated with adverse maternal and child health consequences. Task-shared psychological and psychosocial interventions for perinatal depression have demonstrated clinical and cost-effectiveness when delivered on a large scale. However, task-sharing approaches, especially in LMICs, require an effective mechanism, whereby clients who are not likely to benefit from such interventions are identified from the outset so that they can benefit from higher intensity treatments. Such a stratified approach can ensure that limited resources are utilized appropriately and effectively. The use of standardized and easy-to-implement algorithmic devices (e.g., nomograms) could help with such targeted dissemination of interventions. The present investigation posits a prognostic model and a nomogram to predict the prognosis of perinatal depression among women in rural Pakistan. The nomogram was developed to deliver stratified model of care in primary care settings by identifying those women who respond well to a non-specialist delivered intervention and those requiring specialist care. This secondary analysis utilized data from 903 pregnant women with depression who participated in a cluster randomized, controlled trial that tested the effectiveness of the Thinking Healthy Program in rural Rawalpindi, Pakistan. The participants were recruited from 40 union councils in two sub-districts of Rawalpindi and randomly assigned to intervention and enhanced usual care. Sixteen sessions of the THP intervention were delivered by trained community health workers to women with depression over pregnancy and the postnatal period. A trained assessment team used the Structured Clinical Interview for DSM-IV current major depressive episode module to diagnose major depressive disorder at baseline and post-intervention. The intervention received by the participants emerged as the most significant predictor in the prognostic model. Among clinical factors, baseline severity of core-emotional symptoms emerged as an essential predictor, followed by atypical symptoms and insomnia. Higher severity of these symptoms was associated with a poorer prognosis. Other important predictors of a favorable prognosis included support from one’s mother or mother-in-law, financial empowerment, higher socioeconomic class, and living in a joint family system. This prognostic model yielded acceptable discrimination (c-statistic = 0.75) and calibration to aid in personalized delivery of the intervention.
Modeling Endometrium Biology and Disease
Authors: Maenhoudt Nina , De Moor Amber , Vankelecom Hugo
Published: June 2022

The endometrium, lining the uterine lumen, is highly essential for human reproduction. Its exceptional remodeling plasticity, including the transformation process to welcome and nest the embryo, is not well understood. Lack of representative and reliable study models allowing the molecular and cellular mechanisms underlying endometrium development and biology to be deciphered is an important hurdle to progress in the field. Recently, powerful organoid models have been developed that not only recapitulate endometrial biology such as the menstrual cycle, but also faithfully reproduce diseases of the endometrium such as endometriosis. Moreover, single-cell profiling endeavors of the endometrium in health and disease, and of derived organoids, start to provide deeper insight into cellular complexity and expression specificities, and in resulting tissue processes. This granular portrayal will not only help in understanding endometrium biology and disease, but also in pinning down the tissue’s stem cells, at present not yet conclusively defined. Here, we provide a general overview of endometrium development and biology, and the efforts of modeling both the healthy tissue, as well as its key diseased form of endometriosis. The future of modeling and deciphering this key tissue, hidden inside the womb, looks bright.
The Current State of Radiotherapy for Pediatric Brain Tumors: An Overview of Post-Radiotherapy Neurocognitive Decline and Outcomes
Authors: Major Nicholas , Patel Neal A. , Bennett Josiah , Novakovic Ena , Poloni Dana , Abraham Mickey , Brown Nolan J. , Gendreau Julian L. , Sahyouni Ronald , Loya Joshua
Published: June 2022

Tumors of the central nervous system are the most common solid malignancies diagnosed in children. While common, they are also found to have some of the lowest survival rates of all malignancies. Treatment of childhood brain tumors often consists of operative gross total resection with adjuvant chemotherapy or radiotherapy. The current body of literature is largely inconclusive regarding the overall benefit of adjuvant chemo- or radiotherapy. However, it is known that both are associated with conditions that lower the quality of life in children who undergo those treatments. Chemotherapy is often associated with nausea, emesis, significant fatigue, immunosuppression, and alopecia. While radiotherapy can be effective for achieving local control, it is associated with late effects such as endocrine dysfunction, secondary malignancy, and neurocognitive decline. Advancements in radiotherapy grant both an increase in lifetime survival and an increased lifetime for survivors to contend with these late effects. In this review, the authors examined all the published literature, analyzing the results of clinical trials, case series, and technical notes on patients undergoing radiotherapy for the treatment of tumors of the central nervous system with a focus on neurocognitive decline and survival outcomes.
Marginal Bone Level Evaluation of Fixed Partial Dental Prostheses Using Preformed Stock versus CAD/CAM Customized Abutments
Authors: Lin Hui-Ting , Lin Jerry Chin-Yi , Salamanca Eisner , Dorj Odontuya , Pan Yu-Hwa , Wu Yi-Fan , Hsu Yung-Szu , Fang Chih-Yuan , Chang Wei-Jen
Published: June 2022

Background: The maintenance of marginal bone levels around dental implants is an important criterion for evaluating the success of implants. Although computer-aided design/computer-aided manufacturing (CAD/CAM) customized abutments (CAs) provide more flexible solutions, compared with the original preformed stock abutments (PAs), there are dimensional tolerances and underlying drawbacks in the production of CAD/CAM CAs, which may change the tightness and seamless connection between fixtures and abutments set by the manufacturer and then affect the long-term stability of the abutments. This study aimed to examine the change in both mesial and distal bone levels using digital periapical radiographs to evaluate the difference between CAD/CAM CAs and original PAs.Material and methods: Radiographs were taken before delivery; after functional loading for 1 month; and after 3, 6, and 12 months; and the vertical marginal bone levels (vMBLs) of both the mesial and distal surrounding implant bones were measured. All data are presented as means ± standard errors and were analyzed using Student’s t-test. A p-value < 0.05 was judged to represent a significant difference. Results: A total of 57 implants in 50 patients were divided into 22 CAD/CAM CAs and 35 original stock abutments. The PAs appeared to have a more stable bone level. By contrast, the amount of bone level change in the CAs was higher than that in the PAs. The change in the vMBL of the CAs was significantly more than that of the PAs after functional loading for 1 month (p = 0.006), 3 months (p = 0.013), 6 months (p = 0.014), and 12 months (p = 0.002). In contrast, the distal marginal bone level was lower than the mesial marginal bone level in any period. Nevertheless, the bone levels of the CAs and PAs in any period were comparable with no significant difference. Conclusions: Significant differences were found between the mesial and distal bone levels in the PAs. The CAD/CAM CAs showed a significantly greater bone level change than the original stock abutments after functional loading.
Predicting the 2-Year Risk of Progression from Prediabetes to Diabetes Using Machine Learning among Chinese Elderly Adults
Authors: Liu Qing , Zhou Qing , He Yifeng , Zou Jingui , Guo Yan , Yan Yaqiong
Published: June 2022

Identifying people with a high risk of developing diabetes among those with prediabetes may facilitate the implementation of a targeted lifestyle and pharmacological interventions. We aimed to establish machine learning models based on demographic and clinical characteristics to predict the risk of incident diabetes. We used data from the free medical examination service project for elderly people who were 65 years or older to develop logistic regression (LR), decision tree (DT), random forest (RF), and extreme gradient boosting (XGBoost) machine learning models for the follow-up results of 2019 and 2020 and performed internal validation. The receiver operating characteristic (ROC), sensitivity, specificity, accuracy, and F1 score were used to select the model with better performance. The average annual progression rate to diabetes in prediabetic elderly people was 14.21%. Each model was trained using eight features and one outcome variable from 9607 prediabetic individuals, and the performance of the models was assessed in 2402 prediabetes patients. The predictive ability of four models in the first year was better than in the second year. The XGBoost model performed relatively efficiently (ROC: 0.6742 for 2019 and 0.6707 for 2020). We established and compared four machine learning models to predict the risk of progression from prediabetes to diabetes. Although there was little difference in the performance of the four models, the XGBoost model had a relatively good ROC value, which might perform well in future exploration in this field.
Analysis of Circulating Fatty Acid Profiles in Free-Ranging and Managed Care Marine Toads (Rhinella marina) with a Comparison of Whole-Blood Vial and Whole-Blood Dried Blood Spot Card Analyses
Authors: Witt Melissa L. , Minter Larry J. , Tollefson Troy N. , Ridgley Frank , Treiber Kimberly , Smith Dustin , Bibus Doug , Scott Heather , Ange-van Heugten Kimberly
Published: June 2022

This study provides novel data on circulating concentrations of whole-blood fatty acids (FAs) in marine toads (Rhinella marina) via dried blood spot (DBS) card technology as a potential amphibian model species. Free-ranging (n = 10) animals were compared to managed populations fed two diet strategies for 60 days (n = 6 per diet). Thirty-six individual FAs were analyzed, with 28 found in significant reportable quantities. Eight FA groupings were represented. Traditional whole-blood vial (WBV) FA percentages were also collected and compared to DBS after managed care for 60 days. Results showed eleven individual FAs and four FA groups were higher in free-ranging toads (n = 10; p ≤ 0.05), while three FAs and three groups were higher in managed care (n = 12; p ≤ 0.05). FA concentrations compared between DBS cards and WBV at day 60 generally agreed, although two individual FAs and one grouping were higher in DBS (p ≤ 0.05). When free-ranging FAs were analyzed by sex, four individual FAs and two groupings were higher in females, while four individual FAs and one grouping were higher in males. Understanding normal FA circulating levels and how husbandry changes them may impact amphibian health. Additionally, DBS cards may provide a convenient sampling tool for fieldwork.
Coastal Dynamics Initiate, Relocate and Terminate Short-Lived Wetlands of Dune Slacks, Manawatū, New Zealand
Authors: Villacís Lozada Sylvia P. , Rapson Gillian L.
Published: June 2022

Dunelands are one of the most dynamic environments on Earth, which greatly hinders their conservation and management. In the dune slacks along the Manawatū coast, New Zealand, lies a zone of small, temporary, freshwater wetlands that host early seral communities of rare turf plants. Analysis of historical aerial photos allowed determination of coastline movement, distance of the wetlands from the coast, and wetland movements through time. Study sites were around the coastal settlements of Tangimoana and Foxton Beach, both having major rivers debouching nearby, and Himatangi, amongst stabilising exotic pine plantations. The coastline is prograding (with seaward movement) generally by 0.7–1 m yr−1, but is more variable closer to river mouths, with episodes of movement of up to 15 m yr−1. Wetlands occur 200–400 m behind the strandline, are closer to the coast at Foxton Beach, and furthest away at Himatangi. Wetlands wobble in their position at 5.2 m yr−1 but migrate inland at an average of 2.7 m yr−1. Wetland size appears unrelated to rainfall, but may be related positively to coastal progradation rate, to which wetland movement is negatively related. Near the major rivers, dunes are less stable, and wetlands can be impacted both positively and negatively. Wetland existence and movement is balanced between stability and dynamism on the coast, and management will need to be proactive to maintain environments for early successional turfs.
Strain Development in Microalgal Biotechnology—Random Mutagenesis Techniques
Authors: Bleisch Richard , Freitag Leander , Ihadjadene Yob , Sprenger Una , Steingröwer Juliane , Walther Thomas , Krujatz Felix
Published: June 2022

Microalgal biomass and metabolites can be used as a renewable source of nutrition, pharmaceuticals and energy to maintain or improve the quality of human life. Microalgae’s high volumetric productivity and low impact on the environment make them a promising raw material in terms of both ecology and economics. To optimize biotechnological processes with microalgae, improving the productivity and robustness of the cell factories is a major step towards economically viable bioprocesses. This review provides an overview of random mutagenesis techniques that are applied to microalgal cell factories, with a particular focus on physical and chemical mutagens, mutagenesis conditions and mutant characteristics.
Demyelination Lesions Do Not Correlate with Clinical Manifestations by Bordetella pertussis Toxin Concentrations
Authors: Perussolo Maiara Carolina , Mogharbel Bassam Felipe , Saçaki Claudia Sayuri , Dziedzic Dilcele Silva Moreira , Nagashima Seigo , de Meira Leanderson Franco , Guarita-Souza Luiz Cesar , de Noronha Lúcia , Athayde Teixeira de Carvalho Katherine
Published: June 2022

Multiple sclerosis (MS) is an autoimmune disease of the central nervous system, characterized as an inflammatory demyelinating disease. Given the need for improvements in MS treatment, many studies are mainly conducted through preclinical models such as experimental allergic encephalomyelitis (EAE). This study analyzes the relationships between histopathological and clinical score findings at EAE. Twenty-three female Rattus norvegicus Lewis rats from 6 to 8 weeks were induced to EAE. Nineteen rats underwent EAE induction distributed in six groups to establish the evolution of clinical signs, and four animals were in the control group. Bordetella pertussis toxin (PTX) doses were 200, 250, 300, 350 and 400 ng. The clinical scores of the animals were analyzed daily, from seven to 24 days after induction. The brains and spinal cords were collected for histopathological analyses. The results demonstrated that the dose of 250 ng of PTX induced a higher clinical score and reduction in weight. All induced groups demonstrated leukocyte infiltration, activation of microglia and astrocytes, and demyelinated plaques in the brains in histopathology. It was concluded that the dose of 250 ng and 350 ng of PTX were the best choices to trigger the brain and spinal cord demyelination lesions and did not correlate with clinical scores.
Iron Overload and Breast Cancer: Iron Chelation as a Potential Therapeutic Approach
Authors: Islam Sufia , Hoque Nazia , Nasrin Nishat , Hossain Mehnaz , Rizwan Farhana , Biswas Kushal , Asaduzzaman Muhammad , Rahman Sabera , Hoskin David W. , Sultana Saki , Lehmann Christian
Published: June 2022

Breast cancer has historically been one of the leading causes of death for women worldwide. As of 2020, breast cancer was reported to have overtaken lung cancer as the most common type of cancer globally, representing an estimated 11.3% of all cancer diagnoses. A multidisciplinary approach is taken for the diagnosis and treatment of breast cancer that includes conventional and targeted treatments. However, current therapeutic approaches to treating breast cancer have limitations, necessitating the search for new treatment options. Cancer cells require adequate iron for their continuous and rapid proliferation. Excess iron saturates the iron-binding capacity of transferrin, resulting in non-transferrin-bound iron (NTBI) that can catalyze free-radical reactions and may lead to oxidant-mediated breast carcinogenesis. Moreover, excess iron and the disruption of iron metabolism by local estrogen in the breast leads to the generation of reactive oxygen species (ROS). Therefore, iron concentration reduction using an iron chelator can be a novel therapeutic strategy for countering breast cancer development and progression. This review focuses on the use of iron chelators to deplete iron levels in tumor cells, specifically in the breast, thereby preventing the generation of free radicals. The inhibition of DNA synthesis and promotion of cancer cell apoptosis are the targets of breast cancer treatment, which can be achieved by restricting the iron environment in the body. We hypothesize that the usage of iron chelators has the therapeutic potential to control intracellular iron levels and inhibit the breast tumor growth. In clinical settings, iron chelators can be used to reduce cancer cell growth and thus reduce the morbidity and mortality in breast cancer patients.
Immediate Evaluation of the Effect of Infrared LED Photobiomodulation on Childhood Sleep Bruxism: A Randomized Clinical Trial
Authors: Kobayashi Fernanda Yukie , Castelo Paula Midori , Politti Fabiano , Rocha Monise Mendes , Beltramin Rafael Zaratin , Salgueiro Mônica Da Consolação Canuto , Gonçalves Marcela Leticia Leal , Nammour Samir , Brugnera Júnior Aldo , Sfalcin Ravana Angelini , Bussadori Sandra Kalil
Published: June 2022

The gold standard for the management of sleep bruxism (SB) is the use of a rigid occlusal splint; however, there are limitations for its use in children and alternatives to the management of SB are needed. Photobiomodulation therapy has been used with positive results in temporomandibular disorders. This study aimed to evaluate the effects of photobiomodulation therapy with infrared LED in children with SB. Thirty children were divided into three groups: Group 1: control/absence of bruxism (n = 10); Group 2: SB treated with infrared LED (n = 10); Group 3: SB treated with occlusal splint (n = 10). Electromyographic evaluation of masseter, anterior temporalis, and upper trapezius, and salivary dopamine levels were assessed before and after treatments. Data were statistically analyzed using two-way mixed model ANOVA. An increase in the temporalis and right masseter EMG activity at rest was observed in Group 3, with large effect size (p < 0.05). Left masseter and temporalis EMG activity did not differ over time in the LED group, similar to the control group. Moreover, the EMG activity of masticatory muscles during chewing and upper trapezius muscle did not differ over time in all groups. The results also pointed to a difference in the levels of dopamine between children with and without SB, with Group 3 showing higher levels in the pre-treatment time compared to controls (p < 0.025). In conclusion, an increase in the masticatory muscles activity at rest was observed in children undergoing splint therapy. Moreover, a difference in the levels of salivary dopamine was found between children with and without SB.
Effectiveness and Safety of a New Nutrient Fixed Combination Containing Pollen Extract Plus Teupolioside, in the Management of LUTS in Patients with Benign Prostatic Hypertrophy: A Pilot Study
Authors: Muraca Lucia , Scuteri Antonio , Burdino Elisabetta , Marcianò Gianmarco , Rania Vincenzo , Catarisano Luca , Casarella Alessandro , Cione Erika , Palleria Caterina , Colosimo Manuela , Cutruzzolà Antonio , Vocca Cristina , Basile Emanuele , Citraro Rita , Marsala Gabriella , Di Mizio Giulio , De Sarro Giovambattista , Gallelli Luca
Published: June 2022

Benign prostatic hyperplasia (BPH) is a common cause of male lower urinary tract symptoms (LUTS) that can reduce quality of life. Even if several drugs can be used in its treatment, the development of adverse drug reactions (ADRs) represents the most common cause of low adherence. In the present study, we evaluate both the efficacy and the safety of a new nutrient fixed combination of Pollen Extract plus Teupolioside, named Xipag®, in patients with LUTS. We conduct a pilot single center open label clinical study between 1 March 2020 and 30 June 2020 in patients with BPH referred to general practitioner’s ambulatories. Male patients > 45 years, sexually active, with clinical symptoms of LUTS and with a diagnosis of HPB were enrolled and received one tablet/day of Xipag® (T0), for three months (T1: end of treatment). The IPSS and IIEF-5 questionnaires were carried out at T0 and T1 and represent the first end point, whereas the primary safety end point was considered the absence of ADR or of drug–drug interactions related to Xipag® administration. During the study period, 25 subjects aged 43 to 76 years (mean 62.7 ± 9) were enrolled and completed the study. The clinical evaluation in T1 documented that Xipag® induced a statistically significant improvement (p < 0.01) in symptoms, as documented by the IPSS questionnaire (range 22.7–88.9; mean 55.2 ± 23.6), without the development of ADRs. In conclusion, this is the first real-world study that showed the efficacy and the safety of Xipag® in the BPH patients with LUTS.
Protocol of an Exploratory Single-Arm Study to Evaluate the Safety and Immunogenicity of KD-414 as a Booster Vaccine for SARS-CoV-2 in Healthy Adults (KAPIVARA)
Authors: Terayama Yuriko , Tomita Noriko , Terada-Hirashima Junko , Uemura Yukari , Shimizu Yosuke , Takeuchi Junko S. , Takamatsu Yuki , Maeda Kenji , Mikami Ayako , Ujiie Mugen , Sugiura Wataru
Published: June 2022

Background: The coronavirus disease 2019 (COVID-19) pandemic is currently ongoing, and there have been significant efforts in the development of COVID-19 vaccines. However, the neutralizing antibody titers in vaccinated individuals are reported to progressively decrease over time. Japanese pharmaceutical companies have published the results of Phase I and II studies on the safety and efficacy of different vaccines. Final clinical trials will be conducted with the aim of practical application by March 2023. To effectively utilize vaccines developed by Japanese companies, the efficacy and safety of a booster dose (i.e., third vaccination) must be evaluated among individuals who have received three doses of different vaccines. Methods: This protocol describes a study that aims to examine the effect of a booster dose of “KD-414”, a novel Japanese inactivated vaccine, on antibody titers among participants involved in a previous study. Volunteers in this protocol will be recruited from participants in the previous study and immunized with KD-414 after obtaining consent. The antibody titers, before and after immunization with KD-414, among participants who previously received two doses of the BNT162b2 mRNA vaccine, will be comparatively analyzed. Discussion: The reactogenicity and immunogenicity of seven different COVID-19 vaccines including an inactivated vaccine as a third dose after two doses of ChAdOx1 nCov-19 or BNT162b2, has been tested previously, and found to be superior to control (quadrivalent meningococcal conjugate vaccine) regardless of which vaccine had been received during the initial course. This suggests that many types of third booster doses are efficacious. It is anticipated that this study will provide evidence of the safety and immunogenicity of KD-414 as a booster vaccine, which will have profound public health implications.