bluuebunny/crossref_metadata_2025 huggingface dataset:
TESTE DE EFICÁCIA ENTRE O USO DA HEMOTERAPIA E DA AUTOVACINA COMO PROTOCOLOS DE TRATAMENTOS CONTRA PAPILOMATOSE BOVINA
Authors: Gonçalves Geisiana Barbosa , Carneiro Yuri Faria , Amâncio de Lima Anderson Eduardo , Oliveira Deborah Souza , Da Costa Silva Flávia Rejane , Urzeda Mirianny , Souza Wesley Jose
Published: August 2019

Os tratamentos empregados no controle da papilomatose cutânea bovina foram a auto-hemoterapia e auto-vacina, utilizando-se 24 animais, de diferentes propriedades, próximas a Urutaí, Goiás. Os bovinos foram divididos em três grupos, com oito animais em cada, sendo um grupo submetido ao tratamento com a autovacina, o outro a hemoterapia e um grupo controle. Todos os grupos foram tratados com um intervalo de sete dias entre as aplicações, consecutivamente, durante quatro semanas, onde permaneceram nas devidas propriedades de origem. Procedeu-se à avaliação pelo registro de fotos para comparação e contagem dos papilomas nas áreas mais afetadas. Os animais tratados com ambos os protocolos, apresentaram melhores respostas do que os bovinos pertencentes ao grupo controle. A auto-hemoterapia se mostrou mais eficaz em animais com grau de infecção leve, já a auto-vacina demonstrou ter maior eficácia, em todos os animais, independente do grau da infestação do papiloma, considerando importante uma nova pesquisa, com um tempo de tratamento maior.
Repair Process Analysis for Wind Turbines Equipped with Hydraulic Pitch Mechanism on the U.S. Market in Focus of Cost Optimization
Authors: Kocsis Gergely , Xydis George
Published: August 2019

In recent years both the demand and supply for upgrade solutions and repair services are growing. The majority of the American turbine owners are motivated to be able to operate their various fleets of wind turbines on their own and gain sufficient knowledge to do so in a professional manner. With this goal in mind, the learning curve includes optimizing operation cost, fine-tuning practices, and building a network with suppliers. This work focused on hydraulic pitch system designed for a modern wind turbine, technology overview, and economic loss due to leakage. Furthermore, the work covers the drivers of the customers, performance requirements, and evaluation the current solutions that are available on the market. The possible solutions are listed for each scenario and follow up actions are suggested on how to control future processes. The paper describes how to optimize the long-term running costs and discuss on the changes that could maximize the availability percentage. The findings can be also applied to both other platforms in the product line and even other Original equipment manufacturers’ (OEM) turbines to a certain extent.
Intelligibility and Listening Effort of Spanish Oesophageal Speech
Authors: Raman Sneha , Serrano Luis , Winneke Axel , Navas Eva , Hernaez Inma
Published: August 2019

Communication is a huge challenge for oesophageal speakers, be it for interactions with fellow humans or with digital voice assistants. We aim to quantify these communication challenges (both human–human and human–machine interactions) by measuring intelligibility and Listening Effort (LE) of Oesophageal Speech (OS) in comparison to Healthy Laryngeal Speech (HS). We conducted two listening tests (one web-based, the other in laboratory settings) to collect these measurements. Participants performed a sentence recognition and LE rating task in each test. Intelligibility, calculated as Word Error Rate, showed significant correlation with self-reported LE ratings. Speaker type (healthy or oesophageal) had a major effect on intelligibility and effort. More LE was reported for OS compared to HS even when OS intelligibility was close to HS. Listeners familiar with OS reported less effort when listening to OS compared to nonfamiliar listeners. However, such advantage of familiarity was not observed for intelligibility. Automatic speech recognition scores were higher for OS compared to HS.
Study on Improving the Fixation Rate of Impregnated Poplar Wood with Maltodextrin and 1,3-Dimethylol-4,5-Dihydroxyethyleneurea
Authors: Cai Mingzhen , Fu Zongying , Cai Yingchun , Zhang Yue
Published: August 2019

The impregnation of poplar wood (Populus adenopoda Maxim) with 1,3-dimethylol-4,5-dihydroxyethyleneurea and maltodextrin and the effects of ZnCl2 and curing at 103 °C and 120 °C on the fixation rate and the leaching resistance of modified samples were investigated (103 °C curing, ZnCl2 + 103 °C curing, 120 °C curing, and ZnCl2 + 120 °C curing are denoted as 103, ZC-103, 120, and ZC-120, respectively), with the aim of improving the modification effect. The results showed that ZC-103 had the highest fixation rate, and its weight leaching ratio was higher than that of 120. Fourier-transform infrared spectroscopy showed that ZnCl2 did not affect the functional groups of the modified chemicals. The flexural strength and modulus and the compressive strength perpendicular to the grain were highest for ZC-103. In summary, ZC-103 exhibited the highest fixation rate, indicating that the hardener ZnCl2 bridged and increased the interfacial properties between the chemicals and cell walls and therefore increased the potential for macromolecule polycondensation between the chemicals and cell wall materials. This research paves the way for improving the fixation rate of impregnated wood and provides new insights into practical applications.
Optical Lensless-Camera Communications Aided by Neural Network
Authors: Zhong Suhua , Zhu Yuhong , Chi Xuefen , Shi Hanyang , Sun Hongliang , Wang Shuang
Published: August 2019

Currently, the optical components of a camera embedded in the device constrain its overall thickness. Moreover, if the camera is strongly shaken, the lens and sensor may be misaligned, resulting in a defocusing effect. In this paper, we propose a novel lensless-camera communication model, which removes the lens of camera, therefore decreasing the overall thickness of the device without affecting communications. To decode the images captured by the lensless camera, a decoding algorithm aided by back propagation (BP) neural network was designed, which recognizes the blurred image patterns efficiently. To adapt to time-varying environments, an adaptive training sequence adjustment mechanism was designed. Simulation results show that the proposed image decoding algorithm presents a good bit-error-rate (BER) performance. The proposed system has robust movements and provides resilience to interference, benefiting from the neural network and the designed algorithm.
Immunoexpression of Macroh2a in Uveal Melanoma
Authors: Salvatorelli Lucia , Puzzo Lidia , Bartoloni Giovanni , Palmucci Stefano , Longo Antonio , Russo Andrea , Reibaldi Michele , Vinciguerra Manlio , Li Volti Giovanni , Caltabiano Rosario
Published: August 2019

MacroH2A is a histone variant whose expression has been studied in several neoplasms, including cutaneous melanomas (CMs). In the literature, it has been demonstrated that macroH2A.1 levels gradually decrease during CM progression, and a high expression of macroH2A.1 in CM cells relates to a better prognosis. Although both uveal and cutaneous melanomas arise from melanocytes, uveal melanoma (UM) is biologically and genetically distinct from the more common cutaneous melanoma. Metastasis to the liver is a frequent occurrence in UM, and about 40%–50% of patients die of metastatic disease, even with early diagnosis, proper treatment, and close follow-up. We wanted to investigate macroH2A.1 immunohistochemical expression in UM. Our results demonstrated that mH2A.1 expression was higher in metastatic UM (21/23, 91.4%), while only 18/32 (56.3%). UMs without metastases showed mH2A.1 staining. These data could suggest a possible prognostic role for mH2A.1 and could form a basis for developing new pharmacological strategies for UM treatment.
Thin Film-Based Sensors
Authors: Raposo Maria , Ribeiro Paulo A.
Published: August 2019

An increasing number of fields in everyday life require the development and application of ever more modern and efficient chemical sensors and biosensors, namely to be integrated in intelligent control system networks [...]
Cognitive Processes Underlying Reading Improvement during a Rhythm-Based Intervention. A Small-Scale Investigation of Italian Children with Dyslexia
Authors: Cancer Alice , Stievano Giulia , Pace Gabriella , Colombo Alessia , Antonietti Alessandro
Published: August 2019

Music and rhythm-based training programs to improve reading are a novel approach to treatment of developmental dyslexia and have attracted the attention of trainers and researchers. Experimental studies demonstrating poor basic auditory processing abilities in individuals with dyslexia suggest they should be effective. On this basis, the efficacy of a novel rhythm-based intervention, Rhythmic Reading Training (RRT), was recently investigated and found to improve reading skills in Italian children with dyslexia, but its mode of action remains somewhat unclear. In this study, 19 children and preadolescents with dyslexia received 20 sessions of RRT over 10 weeks. Gains in a set of reading-related cognitive abilities—verbal working memory, auditory, and visual attention, and rhythm processing—were measured, along with reading outcomes. Analysis of the specific contribution of cognitive subprocesses to the primary effect of RRT highlighted that reading speed improvement during the intervention was related to rhythm and auditory discrimination abilities as well as verbal working memory. The relationships among specific reading parameters and the neuropsychological profile of participants are discussed.
Low Dielectric Loss and Multiferroic Properties in Ferroelectric/Mutiferroic/Ferroelectric Sandwich Structured Thin Films
Authors: Wu Zhi-Yong , Ma Cai-Bin
Published: August 2019

Bismuth ferrite (BiFeO3) has proven to be promising for a wide variety of microelectric and magnetoelectric devices applications. In this work, a dense (Ba0.65Sr0.35)TiO3(BST)/(Bi0.875Nd0.125)FeO3(BNF)/BST trilayered thin film grown on Pt-coated Si (100) substrates was developed by the rf-sputtering. For comparison, single-layered BNF and BST were also prepared on the same substrates, respectively. The results show that the dielectric loses suppression in BST/BNF/BST trilayered thin films at room temperature but has enhanced ferromagnetic and ferroelectric properties. The remnant polarization (Pr) and coercive electronic field (Ec) were 5.51 μC/cm2 and 18.3 kV/cm, and the remnant magnetization (Mr) and coercive magnetic field (Hc) were 10.1 emu/cm3 and 351 Oe, respectively, for the trilayered film. We considered that the bismuth’s volatilization was limited by BST bottom layers making the Bi/Fe in good station, and the action of BST layer in the charge transfer between BNF thin film and electrode led to the quite low leakage current and enhanced multiferroic property. The origin of the mechanism of the highly enhanced dielectric constant and decreased loss tanδ was discussed.
Skin Improvement Effects of Gardeniae fructus Extract in HaCaT Keratinocytes, B16F10 Melanocytes, and CCD-986sk Fibroblast Cells
Authors: Hong Se Hyang , Ku Jin Mo , Lee Seung Hwan , Shim Ho Jong , Park Dong Sun , Sung Joo Won , Shin Yong Cheol , Ko Seong-Gyu
Published: August 2019

The development of functional cosmetics with skin improvement effects from natural sources is necessary. In this study, the antioxidant, antiwrinkling, moisturizing, and whitening effects of Gardeniae fructus extract (GF) were investigated in keratinocytes, melanocytes, and fibroblast cells. Antioxidant activity was determined by a DPPH free radical scavenging assay. MMP-1, MMP-9, HAS1, and filaggrin mRNA levels were measured by RT-PCR in keratinocytes and fibroblast cells. MITF and tyrosinase protein levels were evaluated by blotting analysis in melanocytes. DPPH free radical activity was investigated to determine whether GF showed dose-dependent inhibitory activity. GF induced the upregulation of HAS1 and filaggrin mRNA expression in keratinocytes and fibroblast cells. GF led to the downregulation of MMP mRNA levels in keratinocytes and fibroblast cells. Western blotting was performed to confirm the whitening-related protein (MITF and tyrosinase) levels induced by GF in melanocytes, and the inhibitory activity was superior to that of the α-MSH used for the comparison test. GF showed marked antioxidant, antiwrinkling, skin moisturizing, and whitening activity in keratinocytes, melanocytes, and fibroblast cells. Through the results of these experiments, the applicability of GF as a natural and functional cosmetic material was verified.
Evaluation of Malignancy Risk in 18F-FDG PET/CT Thyroid Incidentalomas
Authors: Larg Maria-Iulia , Apostu Dragoș , Peștean Claudiu , Gabora Katalin , Bădulescu Iulian Claudiu , Olariu Elena , Piciu Doina
Published: August 2019

Thyroid incidentalomas detected by 18 fluoro-2-deoxy-d-glucose (FDG)-positron emission tomography/computed tomography (PET/CT) are a real challenge for nuclear medicine physicians and clinicians. This study aimed to evaluate the risk of malignancy for patients with focal thyroid incidentalomas (TIs) diagnosed through FDG PET/CT. Data from 6900 patients, with a known primary tumor, who had an FDG PET/CT investigation performed were analyzed for the presence of incidental thyroid uptake. The focal TIs were reported, and the patients were referred for further investigation to the endocrinology department. There were 126 patients (1.82%) who presented with focal thyroid uptake, and for 87 of them, investigations were completed with ultrasonography (US), and for 29 with a fine needle aspiration biopsy (FNAB) procedure. Malignancy was detected in 7.93% (10/126) of cases. An arbitrary cutoff value of four was established for the standard uptake value lean body mass (SUVlbm Max) to differentiate the malignant nodules from the benign ones, and this value was significantly associated with malignancy (p = 0.0168). TIs are not so frequent, but they have a potential malignancy risk, and a proper evaluation is required. Even though SUVlbm Max is a predictive factor for malignancy, the FNAB remains the main diagnostic method for the therapeutic management of these patients.
Comparing the Diagnostic Accuracy of Two Cognitive Screening Instruments in Different Dementia Subtypes and Clinical Depression
Authors: O’Caoimh Rónán , Molloy D. William
Published: August 2019

Short but accurate cognitive screening instruments are required in busy clinical practice. Although widely-used, the diagnostic accuracy of the standardised Mini-Mental State Examination (SMMSE) in different dementia subtypes remains poorly characterised. We compared the SMMSE to the Quick Mild Cognitive Impairment (Qmci) screen in patients (n = 3020) pooled from three memory clinic databases in Canada including those with mild cognitive impairment (MCI) and Alzheimer’s, vascular, mixed, frontotemporal, Lewy Body and Parkinson’s dementia, with and without co-morbid depression. Caregivers (n = 875) without cognitive symptoms were included as normal controls. The median age of patients was 77 (Interquartile = ±9) years. Both instruments accurately differentiated cognitive impairment (MCI or dementia) from controls. The SMMSE most accurately differentiated Alzheimer’s (AUC 0.94) and Lewy Body dementia (AUC 0.94) and least accurately identified MCI (AUC 0.73), vascular (AUC 0.74), and Parkinson’s dementia (AUC 0.81). The Qmci had statistically similar or greater accuracy in distinguishing all dementia subtypes but particularly MCI (AUC 0.85). Co-morbid depression affected accuracy in those with MCI. The SMMSE and Qmci have good-excellent accuracy in established dementia. The SMMSE is less suitable in MCI, vascular and Parkinson’s dementia, where alternatives including the Qmci screen may be used. The influence of co-morbid depression on scores merits further investigation.
Finite-Time Synchronization of Markovian Jumping Complex Networks with Non-Identical Nodes and Impulsive Effects
Authors: Chen Tao , Peng Shiguo , Zhang Zhenhua
Published: August 2019

In this paper, we investigate the finite-time synchronization problem for a class of Markovian jumping complex networks (MJCNs) with non-identical nodes and impulsive effects. Sufficient conditions for the MJCNs are presented based on an M-matrix technique, Lyapunov function method, stochastic analysis technique, and suitable comparison systems to guarantee finite-time synchronization. At last, numerical examples are exploited to illustrate our theoretical results, and they testify the effectiveness of our results for complex dynamic systems.
An Improved Hybrid Particle Swarm Optimization and Tabu Search Algorithm for Expansion Planning of Large Dimension Electric Distribution Network
Authors: Ahmadian Ali , Elkamel Ali , Mazouz Abdelkader
Published: August 2019

Optimal expansion of medium-voltage power networks is a common issue in electrical distribution planning. Minimizing the total cost of the objective function with technical constraints make it a combinatorial problem which should be solved by powerful optimization algorithms. In this paper, a new improved hybrid Tabu search/particle swarm optimization algorithm is proposed to optimize the electric expansion planning. The proposed method is analyzed both mathematically and experimentally and it is applied to three different electric distribution networks as case studies. Numerical results and comparisons are presented and show the efficiency of the proposed algorithm. As a result, the proposed algorithm is more powerful than the other algorithms, especially in larger dimension networks.
A Reactive Power-Voltage Control Strategy of an AC Microgrid Based on Adaptive Virtual Impedance
Authors: Liu Yao , Guan Lin , Guo Fang , Zheng Jianping , Chen Jianfu , Liu Chao , Guerrero Josep M.
Published: August 2019

As an effective carrier of distributed generation, a microgrid is an effective way to ensure that distributed power can be reasonably utilized. However, due to the property of line impedance and other factors in a microgrid, reactive power supplied by distributed generation units cannot be shared rationally. To efficiently improve reactive power sharing, this paper proposes a reactive power-voltage control strategy based on adaptive virtual impedance. This method changes the voltage reference value by adding an adaptive term based on the traditional virtual impedance. Meanwhile, a voltage recovery mechanism was used to compensate the decline of distributed generation (DG) output voltage in the process. MATLAB/Simulink simulations and experimental results show that the proposed controller can effectively improve the steady state performance of the active and reactive power sharing. Finally, the feasibility and effectiveness of the proposed control strategy were verified.
Calibration Proposal for UHF Partial Discharge Measurements at Power Transformers
Authors: Siegel Martin , Coenen Sebastian , Beltle Michael , Tenbohlen Stefan , Weber Marc , Fehlmann Pascal , Hoek Stefan , Kempf Ulrich , Schwarz Robert , Linn Thomas , Fuhr Jitka
Published: August 2019

The continuous, non-intermitted service of electrical grids relies on the reliability of their assets, e.g., power transformers. Local insulation defects can result in serve failures such as breakdowns with severe subsequent costs. The prevention of such events is crucial. Hence, partial discharge (PD) activity at power transformers is evaluated directly in the factory before shipment. Additionally, PD activity can be monitored during service using the ultra-high frequency (UHF) method. In this contribution, a calibration procedure is proposed for the UHF method. The calibration process is required to ensure both, reproducibility and comparability of UHF measurements: Only a calibrated UHF measurement procedure can be introduced supplementary to IEC 60270 in acceptance tests of power transformers. The proposed calibration method considers two factors: The influence of the UHF-antenna’s sensitivity and the PD recorder characteristics including accessories such as cable damping, pre-amplifier, etc. The former is addressed by a characterization of UHF sensors using the standard antenna factor (AF) in a gigahertz transverse electromagnetic (GTEM) cell. The PD recorder’s influence is corrected by using a defined, invariable test signal as reference for all recording devices. A practical evaluation of the proposed calibration procedure is performed in a laboratory setup using different UHF recording devices and UHF sensors using artificial PD signals and real voltage-driven PD sources.
Modeling and Analysis of the Effects of Noise Barrier Shape and Inflow Conditions on Highway Automobiles Emission Dispersion
Authors: Wang Shaoguang , Wang Xiuling
Published: August 2019

Recent research has suggested that noise barriers have significant impacts on near-road automobile emissions reduction. T-shaped noise barriers have better performance on reducing noise than others, however, their effects on automobile emissions reduction are not clear. In this research, commercial software ANSYS®Fluent 19.2 (Ansys Inc., Canonsburg, PA, USA) was applied to simulate the noise barrier shape and different inflow wind shear condition effects on highway automobiles emission dispersion. Various Reynolds Averaged Navier-Stokes (RANS) models were tested. The realizable k-ε turbulence model was selected to simulate the turbulent flow caused by fast moving vehicles on highway based on the comparison results. A non-reacting species transport model was applied to simulate emission dispersion. Results showed that the T-shaped barrier was able to help reduce highway automobiles emission concentration in downstream areas more than the rectangular barrier. An optimized range of the T-shape was proposed; under the inflow condition without wind shear, the noise barrier shape effects on automobiles emission reduction were not significant.
The Contribution of a Whey Protein Film Incorporated with Green Tea Extract to Minimize the Lipid Oxidation of Salmon (Salmo salar L.)
Authors: Castro Frederico V. R. , Andrade Mariana A. , Sanches Silva Ana , Vaz Maria Fátima , Vilarinho Fernanda
Published: August 2019

Active packaging is becoming progressively more significant as a response to the dynamic changes in current consumer demand and market tendencies. Active packaging is projected to interact directly with the packaged food or with the headspace within the package with the aim of maintaining or extending product quality and shelf-life. Aiming for sustainability, the potential application as biodegradable films of whey protein concentrate (WPC) was evaluated. Aromatic plant’s extracts present high antioxidant properties, representing an alternative for synthetic food additives. The main objective of this study was to verify the effectiveness of an edible WPC film incorporated with a plant-based extract on retarding the lipid oxidation of fresh salmon. Green tea extract (GTE) was chosen to be incorporated into the active film. Fresh salmon was packaged with the control film (WPC) and with active film (WPC–GTE). The oxidation level of non-packaged samples and packaged samples were tested for different storage times. Four methods were applied to evaluate lipid oxidation state of fresh salmon: peroxide value, p-anisidine value, thiobarbituric acid reactive substances (TBARS) assay, and monitoring of hexanal. The results obtained in this study indicate that the whey protein active film was successfully produced, and it was effective in delaying lipid oxidation of fresh salmon samples until the 14th day of storage.
The Hunt for Primordial Interactions in the Large-Scale Structures of the Universe
Authors: Biagetti Matteo
Published: August 2019

The understanding of the primordial mechanism that seeded the cosmic structures we observe today in the sky is one of the major goals in cosmology. The leading paradigm for such a mechanism is provided by the inflationary scenario, a period of violent accelerated expansion in the very early stages of evolution of the universe. While our current knowledge of the physics of inflation is limited to phenomenological models which fit observations, an exquisite understanding of the particle content and interactions taking place during inflation would provide breakthroughs in our understanding of fundamental physics at high energies. In this review, we summarize recent theoretical progress in the modeling of the imprint of primordial interactions in the large-scale structures of the universe. We focus specifically on the effects of such interactions on the statistical distribution of dark-matter halos, providing a consistent treatment of the steps required to connect the correlations generated among fields during inflation all the way to the late-time correlations of halos.
Parenting When Children Have Lyme Disease: Fear, Frustration, Advocacy
Authors: Gaudet Emilie M. , Gould Odette N. , Lloyd Vett
Published: August 2019

Increasing numbers of Canadians, including children and adolescents, are being infected with Borrelia burgdorferi and contracting Lyme disease. In the present study, we provided a qualitative analysis of written correspondence produced by 23 parents of children and adolescents with Lyme disease. The goal of this study was to investigate how medical and psychological issues were highlighted by parents describing their family’s Lyme disease experiences. The results suggest a series of four stages in these families where satisfactory treatment had not been obtained over months or years. The experiences of parents evolved from feelings of worry for the child to frustration with the lack of a helpful treatment, to mistrust of physicians’ actions, and, in some case, to a rejection of the conventional health care system as a whole. Improved diagnostic testing and treatment guidelines, as well as family-centered practices of medical care were proposed as important features for improving the experiences of families living with Lyme disease.
How Many Secret Details Could a Systematic Multi-Analytical Study Reveal About the Mysterious Fresco Trionfo della Morte?
Authors: Alberghina Maria Francesca , Schiavone Salvatore , Greco Caterina , Saladino Maria Luisa , Armetta Francesco , Renda Vincenzo , Caponetti Eugenio
Published: August 2019

The “Trionfo della morte” is a detached fresco painting dated at the half of the XV century. Its history is strictly connected with the history of Palermo and it is considered a symbol of the late Gothic period. Some small areas of the fresco were analyzed using a combination of non-invasive techniques and hand-held instrumentations (multispectral imaging analysis, X-ray fluorescence (XRF), and IR spectroscopy). The characterization of the nature of pigments used in its realization and restoration works was performed and some indications about its conservation state were obtained. More interestingly, some hidden details were revealed on the mysterious painting. They constitute additional evidence of the preciousness of the fresco.
Activation of the Transcription of BrGA20ox3 by a BrTCP21 Transcription Factor Is Associated with Gibberellin-Delayed Leaf Senescence in Chinese Flowering Cabbage during Storage
Authors: Xiao Xian-mei , Xu Yan-mei , Zeng Ze-xiang , Tan Xiao-li , Liu Zong-li , Chen Jian-wen , Su Xin-guo , Chen Jian-ye
Published: August 2019

Several lines of evidence have implicated the involvement of the phytohormone gibberellin (GA) in modulating leaf senescence in plants. However, upstream transcription factors (TFs) that regulate GA biosynthesis in association with GA-mediated leaf senescence remain elusive. In the current study, we report the possible involvement of a TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) TF BrTCP21 in GA-delayed leaf senescence in Chinese flowering cabbage. Exogenous GA3 treatment maintained a higher value of maximum PSII quantum yield (Fv/Fm) and total chlorophyll content, accompanied by the repression of the expression of senescence-associated genes and chlorophyll catabolic genes, which led to the delay of leaf senescence. A class I member of TCP TFs BrTCP21, was further isolated and characterized. The transcript level of BrTCP21 was low in senescing leaves, and decreased following leaf senescence, while GA3 could keep a higher expression level of BrTCP21. BrTCP21 was further found to be a nuclear protein and exhibit trans-activation ability through transient-expression analysis in tobacco leaves. Intriguingly, the electrophoretic mobility shift assay (EMSA) and transient expression assay illustrated that BrTCP21 bound to the promoter region of a GA biosynthetic gene BrGA20ox3, and activated its transcription. Collectively, these observations reveal that BrTCP21 is associated with GA-delayed leaf senescence, at least partly through the activation of the GA biosynthetic pathway. These findings expand our knowledge on the transcriptional mechanism of GA-mediated leaf senescence.
Cytomegalovirus (CMV) Pneumonitis: Cell Tropism, Inflammation, and Immunity
Authors: Fonseca Brito Luís , Brune Wolfram , Stahl Felix R.
Published: August 2019

Human cytomegalovirus (HCMV) is an opportunistic pathogen causing disease mainly in immunocompromised patients or after congenital infection. HCMV infection of the respiratory tract leads to pneumonitis in the immunocompromised host, which is often associated with a bad clinical course. The related mouse cytomegalovirus (MCMV) likewise exhibits a distinct tropism for the lung and thus provides an elegant model to study host-pathogen interaction. Accordingly, fundamental features of cytomegalovirus (CMV) pneumonitis have been discovered in mice that correlate with clinical data obtained from humans. Recent studies have provided insight into MCMV cell tropism and localized inflammation after infection of the respiratory tract. Accordingly, the nodular inflammatory focus (NIF) has been identified as the anatomical correlate of immune control in lungs. Several hematopoietic cells involved in antiviral immunity reside in NIFs and their key effector molecules have been deciphered. Here, we review what has been learned from the mouse model with focus on the microanatomy of infection sites and antiviral immunity in MCMV pneumonitis.
A tRNA-mimic Strategy to Explore the Role of G34 of tRNAGly in Translation and Codon Frameshifting
Authors: Janvier Aurélie , Despons Laurence , Schaeffer Laure , Tidu Antonin , Martin Franck , Eriani Gilbert
Published: August 2019

Decoding of the 61 sense codons of the genetic code requires a variable number of tRNAs that establish codon-anticodon interactions. Thanks to the wobble base pairing at the third codon position, less than 61 different tRNA isoacceptors are needed to decode the whole set of codons. On the tRNA, a subtle distribution of nucleoside modifications shapes the anticodon loop structure and participates to accurate decoding and reading frame maintenance. Interestingly, although the 61 anticodons should exist in tRNAs, a strict absence of some tRNAs decoders is found in several codon families. For instance, in Eukaryotes, G34-containing tRNAs translating 3-, 4- and 6-codon boxes are absent. This includes tRNA specific for Ala, Arg, Ile, Leu, Pro, Ser, Thr, and Val. tRNAGly is the only exception for which in the three kingdoms, a G34-containing tRNA exists to decode C3 and U3-ending codons. To understand why G34-tRNAGly exists, we analysed at the genome wide level the codon distribution in codon +1 relative to the four GGN Gly codons. When considering codon GGU, a bias was found towards an unusual high usage of codons starting with a G whatever the amino acid at +1 codon. It is expected that GGU codons are decoded by G34-containing tRNAGly, decoding also GGC codons. Translation studies revealed that the presence of a G at the first position of the downstream codon reduces the +1 frameshift by stabilizing the G34•U3 wobble interaction. This result partially explains why G34-containing tRNAGly exists in Eukaryotes whereas all the other G34-containing tRNAs for multiple codon boxes are absent.
Effect of the Large and Small T-Antigens of Human Polyomaviruses on Signaling Pathways
Authors: Moens Ugo , Macdonald Andrew
Published: August 2019

Viruses are intracellular parasites that require a permissive host cell to express the viral genome and to produce new progeny virus particles. However, not all viral infections are productive and some viruses can induce carcinogenesis. Irrespective of the type of infection (productive or neoplastic), viruses hijack the host cell machinery to permit optimal viral replication or to transform the infected cell into a tumor cell. One mechanism viruses employ to reprogram the host cell is through interference with signaling pathways. Polyomaviruses are naked, double-stranded DNA viruses whose genome encodes the regulatory proteins large T-antigen and small t-antigen, and structural proteins that form the capsid. The large T-antigens and small t-antigens can interfere with several host signaling pathways. In this case, we review the interplay between the large T-antigens and small t-antigens with host signaling pathways and the biological consequences of these interactions.
Innovative Approaches for Geometric Uncertainty Quantification in an Operational Oil Spill Modeling System
Authors: Feng Dongyu , Passalacqua Paola , Hodges Ben R.
Published: August 2019

Reliable and rapid real-time prediction of likely oil transport paths is critical for decision-making from emergency response managers and timely clean-up after a spill. As high-resolution hydrodynamic models are slow, operational oil spill systems generally rely on relatively coarse-grid models to provide quick estimates of the near-future surface-water velocities and oil transport paths. However, the coarse grid resolution introduces model structural errors, which have been called “geometric uncertainty”. Presently, emergency response managers do not have readily-available methods for estimating how geometric uncertainty might affect predictions. This research develops new methods to quantify geometric uncertainty using fine- and coarse-grid models within a lagoonal estuary along the coast of the northern Gulf of Mexico. Using measures of geometric uncertainty, we propose and test a new data-driven uncertainty model along with a multi-model integration approach to quantify this uncertainty in an operational context. The data-driven uncertainty model is developed from a machine learning algorithm that provides a priori assessment of the prediction’s confidence degree. The multi-model integration generates ensemble predictions through comparison with limited fine-grid predictions. The two approaches provide explicit information on the expected scale of modeling errors induced by geometric uncertainty in a manner suitable for operational modeling.
Examining the Distribution of Strength across the Thickness of Reinforced Concrete Elements Subject to Sulphate Corrosion Using the Ultrasonic Method
Authors: Stawiski Bohdan , Kania Tomasz
Published: August 2019

Sulphate corrosion of concrete is a complex chemical and physical process that leads to the destruction of construction elements. Degradation of concrete results from the transportation of sulphate compounds through the pores of exposed elements and their chemical reactions with cementitious material. Sulphate corrosion can develop in all kind of structures exposed to the corrosive environment. The mechanism of the chemical reactions of sulphate ions with concrete compounds is well known and described. Furthermore, the dependence of the compressive strength of standard cubic samples on the duration of their exposure in the sulphate corrosion environment has been described. However, strength tests on standard samples presented in the scientific literature do not provide an answer to the question regarding the measurement methodology and actual distribution of compressive strength in cross-section of reinforced concrete structures exposed to sulphate ions. Since it is difficult to find any description of this type of test in the literature, the authors undertook to conduct them. The ultrasonic method using exponential heads with spot surface of contact with the material was chosen for the measurements of concrete strength in close cross-sections parallel to the corroded surface. The test was performed on samples taken from compartments of a reinforced concrete tank after five years of operation in a corrosive environment. Test measurements showed heterogeneity of strength across the entire thickness of the tested elements. It was determined that the strength of the elements in internal cross-sections of the structure was up to 80% higher than the initial strength. A drop in the mechanical properties of concrete was observed only in the close zone near the exposed surface.
Preliminary Characterization of Novel LDPE-Based Wear-Resistant Composite Suitable for FDM 3D Printing
Authors: Olesik Piotr , Godzierz Marcin , Kozioł Mateusz
Published: August 2019

Low-density polyethylene (LDPE) composites reinforced with finely powdered waste glass were identified as a potential material for 3D printed structures for use in low-duty frictional applications. A recently published 3D printing model was used to calculate the limits in the filament feed rate and printing speed. Tribological tests (pin-on-disc method) of the printed composites were performed for different print-path directions. Differential scanning calorimetry (DSC) was performed on the samples and the composites showed a higher crystallinity compared with LDPE, which partially explains the higher elastic modulus of the composites determined during static tensile tests. Using a fine glass powder as reinforcement improved the wear resistance of LDPE by 50% due to the formation of a sliding film on the sample’s surface. An evident effect of friction direction vs. the printed path direction on wear was found; which was likely related to differences in the removal of friction products from the friction area for different print-path directions. The LDPE composites with fine waste glass particles are promising materials for low-duty frictional applications and should be the subject of further research.
FEM-Based Study of Precision Hard Turning of Stainless Steel 316L
Authors: Elkaseer Ahmed , Abdelaziz Ali , Saber Mohammed , Nassef Ahmed
Published: August 2019

This study aims to investigate chip formation and surface generation during the precision turning of stainless steel 316L samples. A Finite Element Method (FEM) was used to simulate the chipping process of the stainless steel but with only a restricted number of process parameters. A set of turning tests was carried out using tungsten carbide tools under similar cutting conditions to validate the results obtained from the FEM for the chipping process and at the same time to experimentally examine the generated surface roughness. These results helped in the analysis and understanding the chip formation process and the surface generation phenomena during the cutting process, especially on micro scale. Good agreement between experiments and FEM results was found, which confirmed that the cutting process was accurately simulated by the FEM and allowed the identification of the optimum process parameters to ensure high performance. Results obtained from the simulation revealed that, an applied feed equals to 0.75 of edge radius of new cutting tool is the optimal cutting conditions for stainless steel 316L. Moreover, the experimental results demonstrated that in contrast to conventional turning processes, a nonlinear relationship was found between the feed rate and obtainable surface roughness, with a minimum surface roughness obtained when the feed rate laid between 0.75 and 1.25 times the original cutting edge radius, for new and worn tools, respectively.
Modeling of the Influence of Chemical Composition, Sintering Temperature, Density, and Thickness in the Light Transmittance of Four Zirconia Dental Prostheses
Authors: Fonseca Yuri Resende , Elias Carlos Nelson , Monteiro Sergio Neves , dos Santos Heraldo Elias Salomão , dos Santos Claudinei
Published: August 2019

Research has increasingly been conducted to improve the toughness and aesthetics of zirconium oxide (zirconia) used in prosthetic dentistry. However, the balance between better mechanical properties and greater translucency, to ensure aesthetic requirements, is still a challenge in the development of a novel zirconia for dentistry applications. This study evaluated the transmittance of visible light for four types of pre-sintered zirconia blocks used in dentistry (3Y-SBE, Zpex, Zpex-4, and Zpex-Smile). The objective is to analyze the simultaneous influence of sintering temperature, in the range of 1450–1560 °C, as well as the chemical composition (%Y2O3), density, and thickness (1.0, 1.3, 1.6, and 2.0 mm) in the zirconia’s transmittance. To evaluate the interactive influence of these variables, a statistical learning model based on gradient boosting is applied. The results showed that the effect of the sintering temperature has an optimal (maximum transmittance) point. Increasing the temperature beyond this point reduces the transmittance of the material for all types of zirconia. Moreover, the optimal transmittance point is affected by the chemical composition of each type of zirconia. In addition, the results showed that the transmittance of all types of zirconia had an inverse relationship with the density, zirconia Zpex-Smile being the most sensitive to this parameter. Furthermore, the transmittance of 3Y-SBE, Zpex, and Zpex-4 decreases approximately linearly with the specimen thickness, whereas zirconia Zpex-Smile has a sublinear decrease, which is expected due to the optical isotropy of the cubic phase.
Metabolites from Marine Microorganisms, Micro, and Macroalgae: Immense Scope for Pharmacology
Authors: Barzkar Noora , Jahromi Saeid Tamadoni , Poorsaheli Hadi Bolooki , Vianello Fabio
Published: August 2019

Marine organisms produce a large array of natural products with relevance in drug discovery. These compounds have biological activities such as antioxidant, antibacterial, antitumor, antivirus, anticoagulant, anti-inflammatory, antihypertensive, antidiabetic, and so forth. Consequently, several of the metabolites have made it to the advanced stages of clinical trials, and a few of them are commercially available. In this review, novel information on natural products isolated from marine microorganisms, microalgae, and macroalgae are presented. Given due research impetus, these marine metabolites might emerge as a new wave of promising drugs.
Marine Collagen as A Promising Biomaterial for Biomedical Applications
Authors: Lim Ye-Seon , Ok Ye-Jin , Hwang Seon-Yeong , Kwak Jong-Young , Yoon Sik
Published: August 2019

This review focuses on the expanding role of marine collagen (MC)-based scaffolds for biomedical applications. A scaffold—a three-dimensional (3D) structure fabricated from biomaterials—is a key supporting element for cell attachment, growth, and maintenance in 3D cell culture and tissue engineering. The mechanical and biological properties of the scaffolds influence cell morphology, behavior, and function. MC, collagen derived from marine organisms, offers advantages over mammalian collagen due to its biocompatibility, biodegradability, easy extractability, water solubility, safety, low immunogenicity, and low production costs. In recent years, the use of MC as an increasingly valuable scaffold biomaterial has drawn considerable attention from biomedical researchers. The characteristics, isolation, physical, and biochemical properties of MC are discussed as an understanding of MC in optimizing the subsequent modification and the chemistries behind important tissue engineering applications. The latest technologies behind scaffold processing are assessed and the biomedical applications of MC and MC-based scaffolds, including tissue engineering and regeneration, wound dressing, drug delivery, and therapeutic approach for diseases, especially those associated with metabolic disturbances such as obesity and diabetes, are discussed. Despite all the challenges, MC holds great promise as a biomaterial for developing medical products and therapeutics.
Analysis of Melt-Pool Behaviors during Selective Laser Melting of AISI 304 Stainless-Steel Composites
Authors: Abolhasani Daniyal , Seyedkashi S. M. Hossein , Kang Namhyun , Kim Yang Jin , Woo Young Yun , Moon Young Hoon
Published: August 2019

The melt-pool behaviors during selective laser melting (SLM) of Al2O3-reinforced and a eutectic mixture of Al2O3-ZrO2-reinforced AISI 304 stainless-steel composites were numerically analyzed and experimentally validated. The thermal analysis results show that the geometry of the melt pool is significantly dependent on reinforcing particles, owing to the variations in the melting point and the thermal conductivity of the powder mixture. With the use of a eutectic mixture of Al2O3-ZrO2 instead of an Al2O3 reinforcing particle, the maximum temperature of the melt pool was increased. Meanwhile, a negligible corresponding relationship was observed between the cooling rate of both reinforcements. Therefore, it was identified that the liquid lifetime of the melt pool has the effect on the melting behavior, rather than the cooling rate, and the liquid lifetime increases with the eutectic ratio of Al2O3-ZrO2 reinforcement. The temperature gradient at the top surface reduces with the use of an Al2O3-ZrO2 reinforcement particle due to the wider melt pool. Inversely, the temperature gradient in the thickness direction increases with the use of an Al2O3-ZrO2 reinforcement particle. The results of melt-pool behaviors will provide a deep understanding of the effect of reinforcing particles on the dimensional accuracies and properties of fabricated AISI 304 stainless-steel composites.
Dually Acting Nonclassical 1,4-Dihydropyridines Promote the Anti-Tuberculosis (Tb) Activities of Clofazimine
Authors: Lentz Fabian , Reiling Norbert , Spengler Gabriella , Kincses Annamária , Csonka Andrea , Molnár Joseph , Hilgeroth Andreas
Published: August 2019

The number of effective antituberculotic drugs is strongly limited to four first-line drugs in standard therapy. In case of resistances second-line antibiotics are used with a poor efficacy and tolerability. Therefore, novel antituberculotic drugs are urgently needed. We synthesized novel nonclassical 1,4-dihydropyridines and evaluated their antituberculotic properties depending on substituent effects. Preferred substituents could be identified. As related classical 1,4-dihydropyridines are known as inhibitors of the transmembrane efflux pump ABCB1 in cancer cells, we wondered whether a use of our compounds may be of favour to enhance the antituberculotic drug efficacy of the second-line antituberculotic drug clofazimine, which is a known substrate of ABCB1 by a suggested inhibition of a corresponding efflux pump in Mycobacterium tuberculosis (Mtb). For this, we determined the ABCB1 inhibiting properties of our compounds in a mouse T-lymphoma cell line model and then evaluated the drug-enhancing properties of selected compounds in a co-application with clofazimine in our Mtb strain. We identified novel enhancers of clofazimine toxicity which could prevent clofazimine resistance development mediated by an efflux pump activity.
Halogen and Hydrogen Bonding in Halogenabenzene/NH3 Complexes Compared Using Next-Generation QTAIM
Authors: Li Shuman , Xu Tianlv , van Mourik Tanja , Früchtl Herbert , Kirk Steven R. , Jenkins Samantha
Published: August 2019

Next-generation quantum theory of atoms in molecules (QTAIM) was used to investigate the competition between hydrogen bonding and halogen bonding for the recently proposed (Y = Br, I, At)/halogenabenzene/NH3 complex. Differences between using the SR-ZORA Hamiltonian and effective core potentials (ECPs) to account for relativistic effects with increased atomic mass demonstrated that next-generation QTAIM is a much more responsive tool than conventional QTAIM. Subtle details of the competition between halogen bonding and hydrogen bonding were observed, indicating a mixed chemical character shown in the 3-D paths constructed from the bond-path framework set B. In addition, the use of SR-ZORA reduced or entirely removed spurious features of B on the site of the halogen atoms.