bluuebunny/crossref_metadata_2025 huggingface dataset:
Dengue Fever: Therapeutic Potential of Carica papaya L. Leaves
Authors: Sarker Md. Moklesur Rahman , Khan Farzana , Mohamed Isa Naina
Published: April 2021

Dengue, a very widespread mosquito-borne infectious disease caused by Aedes aegypti virus, has been occurring during the monsoons every year. The prevalence and incidence of dengue fever and death due to its complications have been increased drastically in these recent years in Bangladesh, Philippines, Thailand, Brazil, and India. Recently, dengue had spread in an epidemic form in Bangladesh, Thailand, and Philippines. Although the infection affected a large number of people around the world, there is no established specific and effective treatment by synthetic medicines. In this subcontinent, Malaysia could effectively control its incidences and death of patients using alternative medication treatment mainly prepared from Carica papaya L. leaves along with proper care and hospitalization. Papaya leaves, their juice or extract, as well as their different forms of preparation have long been used traditionally for treating dengue fever and its complications to save patients’ lives. Although it is recommended by traditional healers, and the general public use Papaya leaves juice or their other preparations in dengue fever, this treatment option is strictly denied by the physicians offering treatment in hospitals in Bangladesh as they do not believe in the effectiveness of papaya leaves, thus suggesting to patients that they should not use them. In Bangladesh, 1,01,354 dengue patients have been hospitalized, with 179 deaths in the year 2019 according to information from the Institute of Epidemiology, Disease Control, and Research as well as the Directorate General of Health Services of Bangladesh. Most of the patients died because of the falling down of platelets to dangerous levels and hemorrhage or serious bleeding. Therefore, this paper aims to critically review the scientific basis and effectiveness of Carica papaya L. leaves in treating dengue fever based on preclinical and clinical reports. Thrombocytopenia is one of the major conditions that is typical in cases of dengue infection. Besides, the infection and impairment of immunity are concerned with dengue patients. This review summarizes all the scientific reports on Carica papaya L. for its ability on three aspects of dengue: antiviral activities, prevention of thrombocytopenia and improvement of immunity during dengue fever.
Disease Status–Dependent Drug–Herb Interactions: NASH Lowered the Risk of Hepatotoxicity in Rats Coadministered With Simvastatin and Gardenia jasminoides J. Ellis
Authors: Li Ziwei , Lyu Yuanfeng , Zhao Jiajia , Li Dan , Lin Zhixiu , To Kenneth Kin Wah , Yan Xiaoyu , Zuo Zhong
Published: April 2021

Concurrent use of simvastatin (SV) and Gardenia jasminoides J. Ellis (GJ) was adopted in patients with multi-morbidity, such as stroke rehabilitation patients with NASH. Although hepatotoxicity has been reported in both of them and NASH could alter the pharmacokinetics of drugs/herbs, the interaction between SV and GJ and the related hepatotoxicity remained uninvestigated under neither healthy nor NASH condition. The current study aimed to evaluate the potential hepatotoxicity resulted from the interactions between SV and GJ in both healthy and NASH rats. Both healthy and NASH rats received two-week SV (p. o., 8.66 mg/kg, once daily) and/or GJ (p.o., 325 mg/kg, twice daily). Pharmacokinetic profiles of SV, simvastatin acid (SVA, active metabolite of SV), and geniposide (major component in GJ); hepatic Cyp2c11/Oatp1b2/P-gp expression; and biomarker levels of liver function, lipid levels, and liver histology were compared to demonstrate the interactions in rats. To explore the mechanism of the interaction-mediated hepatotoxicity, hepatic genipin-protein adduct content and iNOS/COX-1/COX-2 expressions from related groups were compared. Moreover, liver histology of healthy/NASH rats at 90 days after discontinuation of two-week GJ in the absence and presence of SV was evaluated to estimate the long-term impact of the interactions. GJ reduced the systemic exposures of SV and SVA by up-regulating the hepatic P-gp expression in healthy but not NASH rats. Meanwhile, SV increased the systemic exposure of geniposide via inhibiting the activity of P-gp in both healthy and NASH rats. Although neither SV nor GJ induced hepatotoxicity in healthy rats, their co-treatment elevated serum ALT and AST levels, which may attribute to the aggravated genipin-protein adduct formation, inflammation infiltration, and iNOS/COX-1 expressions in the liver. In NASH rats, SV and/or GJ reduced serum ALT, AST, LDL/vLDL, and TC levels via alleviating hepatic inflammation infiltration and iNOS/COX-1 expressions. Moreover, in comparison to NASH rats, more severe fibrosis was observed in the livers of healthy rats at 90 days after discontinuation of two-week SV and GJ coadministration. Although interactions between SV and GJ induced short-term and long-term liver injuries in healthy rats, NASH condition in rats could lower such risk.
Nigella Plants – Traditional Uses, Bioactive Phytoconstituents, Preclinical and Clinical Studies
Authors: Salehi Bahare , Quispe Cristina , Imran Muhammad , Ul-Haq Iahtisham , Živković Jelena , Abu-Reidah Ibrahim M. , Sen Surjit , Taheri Yasaman , Acharya Krishnendu , Azadi Hamed , del Mar Contreras María , Segura-Carretero Antonio , Mnayer Dima , Sethi Gautam , Martorell Miquel , Abdull Razis Ahmad Faizal , Sunusi Usman , Kamal Ramla Muhammad , Rasul Suleria Hafiz Ansar , Sharifi-Rad Javad
Published: April 2021

Nigella is a small genus of the family Ranunculaceae, which includes some popular species due to their culinary and medicinal properties, especially in Eastern Europe, Middle East, Western, and Central Asia. Therefore, this review covers the traditional uses and phytochemical composition of Nigella and, in particular, Nigella sativa. The pharmacological studies reported in vitro, in vivo, and in humans have also been reviewed. One of the main strength of the use of Nigella is that the seeds are rich in the omega-6 fatty acid linoleic acid and provide an extra-source of dietary phytochemicals, including the bioactive thymoquinone, and characteristics saponins, alkaloids, and flavonoids. Among Nigella species, N. sativa L. is the most studied plant from the genus. Due to the phytochemical composition and pharmacological properties, the seed and seed oil from this plant can be considered as good candidates to formulate functional ingredients on the basis of folklore and scientific knowledge. Nonetheless, the main limations are that more studies, especially, clinical trials are required to standardize the results, e.g. to establish active molecules, dosage, chemical profile, long-term effects and impact of cooking/incorporation into foods.
Anacardium Occidentale L. Leaf Extracts Protect Against Glutamate/H2O2-Induced Oxidative Toxicity and Induce Neurite Outgrowth: The Involvement of SIRT1/Nrf2 Signaling Pathway and Teneurin 4 Transmembrane Protein
Authors: Duangjan Chatrawee , Rangsinth Panthakarn , Zhang Shaoxiong , Wink Michael , Tencomnao Tewin
Published: April 2021

Neurodegenerative diseases are linked to neuronal cell death and neurite outgrowth impairment that are often caused by oxidative stress. Natural products, which have neuroprotective against oxidative stress and neurite outgrowth inducing activity, could be potential candidates for alternative treatment of neurodegenerative diseases. This study aims to investigate the neuroprotective effects and neuritogenesis properties of Anacardium occidentale leaf extracts in cultured neuronal (HT22 and Neuro-2a) cells. We found gallic acid, catechin and quercetin as the main compounds in A. occidentale extracts. The extracts have a protective effect against glutamate/H2O2-mediated oxidative stress-induced cell toxicity. The gene expression of cellular antioxidant enzymes (SODs, GPx and, GSTs) were up-regulated by this treatment. The treatment also triggered SIRT, Nrf2 proteins as well as the mRNA transcriptions of relevant anti-oxidation genes (NQO1, GCLM, and EAAT3). We demonstrated that the extracts promote antioxidant defense in neuronal cells via the SIRT1/Nrf2 signaling pathway. Moreover, the extracts increase neurite outgrowth and Ten-4 expression in Neuro-2a cells. However, the neuritogenesis properties did not occur, when Ten-4 expression was knocked down by corresponding siRNA. These results suggest that the leaf extracts have an interesting neuritogenesis and neuroprotective potential against glutamate/H2O2-mediated toxicity and could be a potential therapeutic candidate for neurodegenerative diseases.
Knockout of Formyl Peptide Receptor-1 Attenuates Cigarette Smoke–Induced Airway Inflammation in Mice
Authors: Gao Lijuan , Zeng Ni , Yuan Zhicheng , Wang Tao , Chen Lei , Yang Deqing , Xu Dan , Wan Chun , Wen Fuqiang , Shen Yongchun
Published: April 2021

Objective: The formyl peptide receptor-1 (FPR-1) has been reported to be implicated in the regulation of inflammatory disorders, while its role in cigarette smoke (CS)–induced airway inflammation has not been fully explained. In this study, we investigated the role of FPR-1 in CS-induced airway inflammation and the possible mechanism through gene knockout (KO) technology and transcriptional study.

Methods: FPR-1 KO or wild-type C57BL/6 mice were exposed to mainstream CS to establish an airway inflammation model. Cell counts and pro-inflammatory cytokines were measured in bronchoalveolar lavage fluid (BALF). Lung tissues were collected for histological examination, polymerase chain reaction, Western blot, transcriptomic gene study, and related bioinformatics analysis.

Results: CS exposure induced significant histological inflammatory changes, increased neutrophils, and pro-inflammatory cytokines in the BALF of wild-type mice, which were all attenuated by KO of FPR-1. The transcriptomic gene study showed a total of 198 up-regulated genes and 282 down-regulated genes in mouse lungs. Bioinformatics analysis including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) suggested these differentiated expressed genes were significantly related to the immune, chemotaxis responses, and cross-talked with a complicated network of signaling pathways including NF-κB. Western blot validated that KO of FPR-1 inhibited CS-induced NF-κB activation.

Conclusion: Knockout of FPR-1 significantly ameliorates CS-induced airway inflammation in mice, possibly via its related immune-chemotaxis responses and inhibition of NF-κB activation.
Low Quality of Warfarin Therapy is Associated With Female Gender but Not With Polypharmacy in Patients With Atrial Fibrillation
Authors: Takamoto Kojiro , Sakamoto Jun-ichi , Ito Satoyasu , Kimura Takeshi , Manabe Eri , Shikata Toshiyuki , Asakura Masanori , Ishihara Masaharu , Tsujino Takeshi
Published: April 2021

Background: We examined the impact of polypharmacy on the quality of the anticoagulation therapy in patients with atrial fibrillation. We also examined the factors that affect the stability of warfarin therapy.

Methods and Results: This retrospective study was conducted using data from 157 consecutive outpatients with atrial fibrillation in a single tertiary referral hospital. Patients who were prescribed warfarin continuously and for whom PT-INR was examined at least three times in a year were included in this study. We examined the quality of warfarin therapy using time in the therapeutic INR range (TTR), percentage of PT-INR determinations in range (PINRR), and the coefficient variation (CV) of PT-INR. We found that the number of prescribed medicines was significantly associated with high BMI and low eGFR, but not with TTR, PINRR, and the coefficient variation of PT-INR in patients with atrial fibrillation. We also found that female gender was independently associated with low PINRR in this study population.

Conclusion: Polypharmacy did not deteriorate the quality of warfarin therapy in patients with atrial fibrillation treated in the tertiary referral hospital. Female gender was an independent predictor of the low quality of warfarin therapy.
Neuroinflammatory In Vitro Cell Culture Models and the Potential Applications for Neurological Disorders
Authors: Peng Ye , Chu Shifeng , Yang Yantao , Zhang Zhao , Pang Zongran , Chen Naihong
Published: April 2021

Cell cultures are used in pharmaceutical, medical and biological sciences. Due to the ethical and cost limitations of in vivo models, the replaceable cell model that is more closely related to the characteristics of organisms, which has broad prospects and can be used for high-throughput drug screening is urgent. Neuronal and glial cell models have been widely used in the researches of neurological disorders. And the current researches on neuroinflammation contributes to blood-brain barrier (BBB) damage. In this review, we describe the features of healthy and inflamed BBB and summarize the main immortalized cell lines of the central nervous system (PC12, SH-SY5Y, BV2, HA, and HBMEC et al.) and their use in the anti-inflammatory potential of neurological disorders. Especially, different co-culture models of neuroinflammatory, in association with immune cells in both 2D and 3D models are discussed in this review. In summary, 2D co-culture is easily practicable and economical but cannot fully reproduce the microenvironment in vivo. While 3D models called organs-on-chips or biochips are the most recent and very promising approach, which made possible by bioengineering and biotechnological improvements and more accurately mimic the BBB microenvironment.
Perceived Strain Due to COVID-19-Related Restrictions Mediates the Effect of Social Needs and Fear of Missing Out on the Risk of a Problematic Use of Social Networks
Authors: Wegmann Elisa , Brandtner Annika , Brand Matthias
Published: April 2021

The occurrence of the COVID-19-virus led to drastic short-term measures to reduce its spread and influence. Regulations such as “physical distancing,” mentioned as “social distancing,” and the closure of public facilities during the lockdown could be perceived as burdensome especially by individuals who feel a strong need for social exchange and belonging. These components such as need to belong and the fear of missing out also play a major role in the development and maintenance of a problematic use of social networks. Researchers have argued recently that an increase of addictive (online) behaviors may be a likely consequence of subjectively experienced restrictions in the context of the COVID-19 pandemic. The current study investigates the interplay of perceived strain due to COVID-19-related restrictions and the fear of missing out (FoMO) as well as of symptoms of problematic social-networks use. We hypothesized that perceived strain due to COVID-19-related restrictions mediates the effect of specific predisposing variables related to social needs on the symptom severity of a problematic use. To assess the perceived strain due to COVID-19-related restrictions, we developed a specific questionnaire asking for perceived COVID-19-related strain in several domains of everyday-life. An exploratory factor analysis identified five factors: perceived strain related to restrictions of (1) social contacts, (2) travel, (3) childcare, (4) work, and (5) own health. In a sample of 719 German participants and data collection during the first COVID-19 lockdown (March 30th until April 3rd 2020), a structural equation model was calculated showing that higher levels of need to belong and FoMO increase perceived COVID-19-related strain, which is related to symptoms of a problematic social-networks use. The effect of need to belong on problematic social-networks use is mediated by experienced COVID-19-related strain and FoMO-online. Even if the use of social networks is not pathological per se, it may be associated with suffering for a vulnerable part of users. We conclude that specific needs and fear-associated predisposing variables contribute to experiencing physical distance and other pandemic-related restrictions as more stressful, which may increase problematic social-networks use and potentially other addictive behaviors as well in the context of the COVID-19-related lockdown.
ERR and dPECR Suggest a Link Between Neuroprotection and the Regulation of Ethanol Consumption Preference
Authors: Velo Escarcena Laura , Neufeld Margarita , Rietschel Marcella , Spanagel Rainer , Scholz Henrike
Published: April 2021

Reconsumption of ethanol after withdrawal is a hallmark for relapse in recovering patients with alcohol use disorders. We show that the preference of Drosophila melanogaster to reconsume ethanol after abstinence shares mechanistic similarities to human behavior by feeding the antirelapse drug acamprosate to flies and reducing the ethanol consumption preference. The Drosophila cellular stress mutant hangover also reduced ethanol consumption preference. Together with the observation that an increasing number of candidate genes identified in a genome-wide association study on alcohol use disorders are involved in the regulation of cellular stress, the results suggest that cellular stress mechanisms might regulate the level of ethanol reconsumption after abstinence. To address this, we analyzed mutants of candidate genes involved in the regulation of cellular stress for their ethanol consumption level after abstinence and cellular stress response to free radicals. Since hangover encodes a nuclear RNA-binding protein that regulates transcript levels, we analyzed the interactions of candidate genes on transcript and protein level. The behavioral analysis of the mutants, the analysis of transcript levels, and protein interactions suggested that at least two mechanisms regulate ethanol consumption preference after abstinence—a nuclear estrogen-related receptor-hangover-dependent complex and peroxisomal trans-2-enoyl-CoA reductase (dPECR)-dependent component in peroxisomes. The loss of estrogen-like receptor and dPECR in neurons share a protective function against oxidative stress, suggesting that the neuroprotective function of genes might be a predictor for genes involved in the regulation of ethanol reconsumption after abstinence.
Evolutionary Analysis of Pre-S/S Mutations in HBeAg-Negative Chronic Hepatitis B With HBsAg < 100 IU/ml
Authors: Wu Yingping , Zhu Zhiqiang , Wu Jianyong , Bi Wenzi , Xu Wei , Xia Xiaoping , Han Dongsheng
Published: April 2021

Background: Hepatitis B surface antigen (HBsAg) and viral load are important clinical indicators for antiviral therapy. Few studies have evaluated viral sequence biomarkers predicting the risk of hepatocellular carcinoma (HCC) in the stage, which show a low serological response (HBsAg < 100 IU/ml) and high viral levels (HBV DNA > 2,000 IU/ml). This study aims to determine the trend of the biological prevalence within the pre-S/S regions of special model of inactive CHB infection.

Methods: We used Sanger sequencing, quantitative HBV serology (HBeAg and HBsAg), and liver function index to identify whether HBV genome sequences are associated with long-term risk of further HCC progression in special inactive CHB infection.

Results: HBV sequencing analysis of 28 CHB patients with special infectious pattern showed higher genetic diversity among four opening reading frames (ORFs) (p < 0.001). However, dN/dS ratios of HBsAg and pre-C/C regions in the experimental group showed no significantly different from those in the HCC group (p = 0.06), while significantly lower in polymerase and HBxAg regions of the experimental group (p < 0.001). In addition, seven positively selected sites were identified in pre-S1, five in pre-S2, and four in S, in which five sites (128H/135Q/135R/139L/141P) were among “α” determinant.

Conclusions: These mutations in the pre-S/S region might be associated with the HCC phenotype of low HBsAg expression, with the P region possibly impacting high viral loads. Increased viral diversity across the HBV genome is also associated with low levels of HBsAg. The cumulative evolutionary changes in the HBV pre-S/S regions shows that facilitate immune evasion should be monitored individually. Due to the similarity of evolutionary characteristics in HCC, low serological responses and high viremia may be associated with the risk of further disease progression.
Nitrogen Dioxide Selective Sensor for Humid Environments Based on Octahedral Indium Oxide
Authors: Domènech-Gil Guillem , Gràcia Isabel , Cané Carles , Romano-Rodríguez Albert
Published: April 2021

We report the growth of micrometer-sized In2O3 octahedral structures, which are next aligned in chains using dielectrophoresis on top of microhotplates with prepatterned electrodes and integrated heater to work as chemoresistive gas sensors. The devices are relatively fast (180 s), highly sensitive (response up to ~256%), and selective toward NO2 in humid environments, showing little response to O2 and ethanol, and being completely insensitive to CO and CH4. The here-presented fabrication method can be easily extended as a cost-effective post-process in CMOS-compatible microhotplate fabrication and, thus, represents a promising candidate for indoor and outdoor air quality monitoring devices.
Concrete Lessons: Policies and Practices Affecting the Impact of COVID-19 for Urban Indigenous Communities in the United States and Canada
Authors: Howard-Bobiwash Heather A. , Joe Jennie R. , Lobo Susan
Published: April 2021

Throughout the Americas, most Indigenous people move through urban areas and make their homes in cities. Yet, the specific issues and concerns facing Indigenous people in cities, and the positive protective factors their vibrant urban communities generate are often overlooked and poorly understood. This has been particularly so under COVID-19 pandemic conditions. In the spring of 2020, the United Nations High Commissioner Special Rapporteur on the Rights of Indigenous Peoples called for information on the impacts of COVID-19 for Indigenous peoples. We took that opportunity to provide a response focused on urban Indigenous communities in the United States and Canada. Here, we expand on that response and Indigenous and human rights lens to review policies and practices impacting the experience of COVID-19 for urban Indigenous communities. Our analysis integrates a discussion of historical and ongoing settler colonialism, and the strengths of Indigenous community-building, as these shape the urban Indigenous experience with COVID-19. Mindful of the United Nations Declaration on the Rights of Indigenous Peoples, we highlight the perspectives of Indigenous organizations which are the lifeline of urban Indigenous communities, focusing on challenges that miscounting poses to data collection and information sharing, and the exacerbation of intersectional discrimination and human rights infringements specific to the urban context. We include Indigenous critiques of the implications of structural oppressions exposed by COVID-19, and the resulting recommendations which have emerged from Indigenous urban adaptations to lockdown isolation, the provision of safety, and delivery of services grounded in Indigenous initiatives and traditional practices.
Increase in the Value of Agricultural Parcels—Modelling and Simulation of the Effects of Land Consolidation Project
Authors: Dacko Mariusz , Wojewodzic Tomasz , Pijanowski Jacek , Taszakowski Jarosław , Dacko Aneta , Janus Jarosław
Published: April 2021

In the theory and practice of valuation, it is commonly accepted that the key feature determining the value of agricultural land is its location, both general and in a specific part (zone) of a village. The model approach used in the present study can provide the answer to the question of how to maximize the value of agricultural land as part of a conducted arrangement, agricultural works. The study used data on the market sale of agricultural parcels in 10 Polish municipalities. Each parcel was described using a set of features (parameters) that were key to its value and entered into a database. Using the database, two statistical models were built: a multiple regression analysis model (MRA) and an artificial neural network model (ANN). The studies conducted have shown that changes in such features as surface area, shape, and access to a public road were accompanied by significant changes in the market values of parcels. Another important observation was that potential decreases in the value of agricultural parcels as a result of changes in their surface areas were offset (where it was reasonable) by the elimination of their excessive elongation and providing them with an access to a public road. Based on the findings, it has been concluded that change in land value should be considered one of the effects of executed land consolidation projects (LCP), during which the parameters of agricultural parcels are subject to the biggest changes.
Achieving the European Green “Deal” of Sustainable Grass Forage Production and Landscaping Using Fungal Endophytes
Authors: Pańka Dariusz , Jeske Małgorzata , Łukanowski Aleksander , Prus Piotr , Szwarc Katarzyna , Muhire Jean de Dieu
Published: April 2021

The European Green Deal is the EU’s latest growth strategy and action plan, which will meet the challenges concerning climate change and environmental degradation. The components of the Green Deal which are intended to prevent biodiversity loss, to reduce pollution level, and to improve food quality are: The Farm to Fork Strategy and Biodiversity Strategy. Their main aims include: Reducing the application of pesticides by 50% by 2030, reducing nutrients loss by 50% while preserving soil fertility, reducing the application of mineral fertilizers by a minimum of 20% by 2030, as well as supporting the development of organic farming to reach 25% of all the arable land in the EU. These aims are very ambitious and they pose a serious challenge. Can the European Green Deal provide an opportunity for fungal grass endophytes? This paper presented different aspects in which endophytes of the Epichloë genus affect colonized plants, as well as their possible applications in biological grass protection and in improving the performance properties of different grass biotopes. Literature was reviewed to provide evidence of how fungal endophytes might be used to achieve the goals of the European Green Deal strategy, in accordance with the principles of sustainable agriculture.
Noise Reduction Effect of Superhydrophobic Surfaces with Streamwise Strip of Channel Flow
Authors: Niu Chen , Liu Yongwei , Shang Dejiang , Zhang Chao
Published: April 2021

Superhydrophobic surface is a promising technology, but the effect of superhydrophobic surface on flow noise is still unclear. Therefore, we used alternating free-slip and no-slip boundary conditions to study the flow noise of superhydrophobic channel flows with streamwise strips. The numerical calculations of the flow and the sound field have been carried out by the methods of large eddy simulation (LES) and Lighthill analogy, respectively. Under a constant pressure gradient (CPG) condition, the average Reynolds number and the friction Reynolds number are approximately set to 4200 and 180, respectively. The influence on noise of different gas fractions (GF) and strip number in a spanwise period on channel flow have been studied. Our results show that the superhydrophobic surface has noise reduction effect in some cases. Under CPG conditions, the increase in GF increases the bulk velocity and weakens the noise reduction effect. Otherwise, the increase in strip number enhances the lateral energy exchange of the superhydrophobic surface, and results in more transverse vortices and attenuates the noise reduction effect. In our results, the best noise reduction effect is obtained as 10.7 dB under the scenario of the strip number is 4 and GF is 0.5. The best drag reduction effect is 32%, and the result is obtained under the scenario of GF is 0.8 and strip number is 1. In summary, the choice of GF and the number of strips is comprehensively considered to guarantee the performance of drag reduction and noise reduction in this work.
Evaluation of Multivariate Biomarker Indexes Application in Ecotoxicity Tests with Marine Diatoms Exposed to Emerging Contaminants
Authors: Pires Vanessa Leal , Novais Sara C. , Lemos Marco F. L. , Fonseca Vanessa F. , Duarte Bernardo
Published: April 2021

Worldwide anthropogenic activities result in the production and release of potentially damaging toxic pollutants into ecosystems, thereby jeopardizing their health and continuity. Research studies and biomonitoring programs attend to this emerging problematic by applying and developing statistically relevant indexes that integrate complex biomarker response data to provide a holistic approach, reflecting toxically induced alterations at the organism or population level. Ultimately, indexes allow simple result communications, enhancing policy makers understanding, and contributing to better resource and environmental managing policies. In this study three indexes, the integrated biomarker response index (IBR), the bioeffects assessment index (BAI) and principal components analysis (PCA), were evaluated for their sensitivity in revealing toxically induced stress patterns in cells of the diatom Phaeodactylum tricornutum under contaminant exposure. The set of biomarkers selected for index construction comprised the anti-oxidant enzymes APX, CAT and SOD, and the lipid peroxidation marker TBARS. Several significant correlations with the applied concentration gradients were noticed for all indexes, although IBR excelled for its reliability in delivering statistically significant dose-response patterns for four out of the five tested compounds.
Impact of Transparency in the Teamwork Development through Cloud Computing
Authors: Sein-Echaluce María Luisa , Fidalgo-Blanco Angel , García-Peñalvo Francisco José , Fonseca David
Published: April 2021

Active educational methodologies promote students to take an active role in their own learning, enhance cooperative work, and develop a collective understanding of the subject as a common learning area. Cloud Computing enables the learning space to be supported while also revolutionizing it by allowing it to be used as a link between active methodology and students’ learning activities. A Cloud Computing system is used in conjunction with an active methodology to recognize and manage individual, group, and collective evidence of the students’ work in this research. The key hypothesis shown in this work is that if evidence management is made clear and evidence is consistently and gradually presented to students, their level of involvement will increase, and their learning outcomes will improve. The model was implemented in a university subject of a first academic year using the active Flipped Classroom methodology, and the individual, group and collective evidence is constantly worked with throughout the implementation of a teamwork method.
Methodology for Data-Informed Process Improvement to Enable Automated Manufacturing in Current Manual Processes
Authors: Adrita Mumtahina Mahajabin , Brem Alexander , O’Sullivan Dominic , Allen Eoin , Bruton Ken
Published: April 2021

Manufacturing industries are constantly identifying ways to automate machinery and processes to reduce waste and increase profits. Machines that were previously handled manually in non-standardized manners can now be automated. Converting non-digital records to digital formats is called digitization. Data that are analyzed or entered manually are subject to human error. Digitization can remove human error, when dealing with data, via automatic extraction and data conversion. This paper presents methodology to identify automation opportunities and eliminate manual processes via digitized data analyses. The method uses a hybrid combination of Lean Six Sigma (LSS), CRISP-DM framework, and “pre-automation” sequence, which address the gaps in each individual methodology and enable the identification and analysis of processes for optimization, in terms of automation. The results from the use case validates the novel methodology, reducing the implant manufacturing process cycle time by 3.76%, with a 4.48% increase in product output per day, as a result of identification and removal of manual steps based on capability studies. This work can guide manufacturing industries in automating manual production processes using data digitization.
Chloroplast Localized FIBRILLIN11 Is Involved in the Osmotic Stress Response during Arabidopsis Seed Germination
Authors: Choi Yu-Ri , Kim Inyoung , Kumar Manu , Shim Jaekyung , Kim Hyun-Uk
Published: April 2021

Plants live in ever-changing environments, facing adverse environmental conditions including pathogen infection, herbivore attack, drought, high temperature, low temperature, nutrient deficiency, toxic metal soil contamination, high salt, and osmotic imbalance that inhibit overall plant growth and development. Plants have evolved mechanisms to cope with these stresses. In this study, we found that the FIBRILLIN11 (FBN11) gene in Arabidopsis, which has a lipid-binding FBN domain and a kinase domain, is involved in the plant’s response to abiotic stressors, including salt and osmotic stresses. FBN11 protein localizes to the chloroplast. FBN11 gene expression significantly changed when plants were exposed to the abiotic stress response mediators such as abscisic acid (ABA), sodium chloride (NaCl), and mannitol. The seed germination rates of fbn11 homozygous mutants in different concentrations of mannitol and NaCl were significantly reduced compared to wild type. ABA-dependent and -independent stress response regulatory genes were differentially expressed in the fbn11 mutant compared with wild type when grown in mannitol medium. These results suggest a clear role for chloroplast-localized FBN11 in mediating osmotic stress tolerance via the stress response regulatory signaling pathway in the nucleus.
Natural Products in Modern Biology: Ancient Wisdom for Today’s Challenges
Authors: Krüger Marcus , Richter Peter , Strauch Sebastian M.
Published: April 2021

Nature provides a unique diversity of primary and secondary metabolites [...]
Preoperative Devascularization of Choroid Plexus Tumors: Specific Issues about Anatomy and Embolization Technique
Authors: Baro Valentina , Gabrieli Joseph Domenico , Cester Giacomo , D’Errico Ignazio , Landi Andrea , Denaro Luca , Causin Francesco
Published: April 2021

(1) Background: Surgical treatment of choroid plexus tumors is challenging, burdened by a notable risk of bleeding. Neoadjuvant chemotherapy and preoperative embolization have been attempted, with encouraging results; however, the consensus on these procedures is lacking. (2) Methods: We present a case of a 10-month-old girl who underwent preoperative embolization of a hemorrhagic choroid plexus carcinoma of the lateral ventricle via the anterior choroidal artery, followed by total resection. (3) Results: The endovascular procedure was successfully completed, despite the rectification of the anterior choroidal artery associated with the absence of flow proximal to the plexal point. Minimal bleeding was observed during resection and the patient remained neurologically intact. (4) Conclusions: The time from entrance to exit in the anterior choroidal artery should be monitored and regarded as a potential ‘occlusion time’ in this specific group of patients. Nevertheless, our case supports the feasibility and effectiveness of preoperative embolization of a choroid plexus carcinoma of the lateral ventricle via the anterior choroidal artery, without complications. Furthermore, we suggest the use of a fast-embolic agent, such as N-butyl cyanoacrylate glue, as the preferred agent for this specific pathology and patient population.
Cilioretinal Artery Occlusion after Endovascular Coil Embolization for Anterior Communicating Artery
Authors: Bae Heewon , Kang TaeGu , Jeong Da-Eun , Shim KyuHwan , Kang MinJu
Published: April 2021

Unruptured intracranial aneurysms have a risk of rupture, so coil embolization is widely practiced as it preserves a patent artery. There are complications of coil procedures, such as patent artery occlusion and thromboembolism, which can result in retinal artery occlusion. We report onretinal artery occlusion following coil embolization of anterior communicating artery aneurysm. This is a rare case of a combination of cilioretinal and branch retinal artery occlusion, and is unusual in showing a functional recovery.
Tenuazonic Acid-Triggered Cell Death Is the Essential Prerequisite for Alternaria alternata (Fr.) Keissler to Infect Successfully Host Ageratina adenophora
Authors: Shi Jiale , Zhang Min , Gao Liwen , Yang Qian , Kalaji Hazem M. , Qiang Sheng , Strasser Reto Jörg , Chen Shiguo
Published: April 2021

The necrotrophic fungus Alternaria alternata contains different pathotypes that produce different mycotoxins. The pathotype Ageratina adenophora secretes the non-host-selective toxin tenuazonic acid (TeA), which can cause necrosis in many plants. Although TeA is thought to be a central virulence factor of the A. adenophora pathotype, the precise role of TeA in different stages of host infection by pathogens remains unclear. Here, an A. alternata wild-type and the toxin-deficient mutant ΔHP001 with a 75% reduction in TeA production were used. It was observed that wild-type pathogens could induce the reactive oxygen species (ROS) bursts in host leaves and killed photosynthetic cells before invading hyphae. The ROS interceptor catalase remarkably inhibited hyphal penetration and invasive hyphal growth and expansion in infected leaves and suppressed necrotic leaf lesion. This suggests that the production of ROS is critical for pathogen invasion and proliferation and disease symptom formation during infection. It was found that the mutant pathogens did not cause the formation of ROS and cell death in host leaves, showing an almost complete loss of disease susceptibility. In addition, the lack of TeA resulted in a significant reduction in the ability of the pathogen to penetrate invasive hyphal growth and spread. The addition of exogenous TeA, AAL-toxin, and bentazone to the mutant ΔHP001 pathogens during inoculation resulted in a significant restoration of pathogenicity by increasing the level of cell death, frequency of hyphal penetration, and extent of invasive hyphal spread. Our results suggest that cell death triggered by TeA is the essential requirement for successful colonization and disease development in host leaves during infection with A. adenophora pathogens.
Integrated Action of Autophagy and Adipose Tissue Triglyceride Lipase Ameliorates Diet-Induced Hepatic Steatosis in Liver-Specific PLIN2 Knockout Mice
Authors: Griffin John D. , Bejarano Eloy , Wang Xiang-Dong , Greenberg Andrew S.
Published: April 2021

An imbalance in the storage and breakdown of hepatic lipid droplet (LD) triglyceride (TAG) leads to hepatic steatosis, a defining feature of non-alcoholic fatty liver disease (NAFLD). The two primary cellular pathways regulating hepatic TAG catabolism are lipolysis, initiated by adipose triglyceride lipase (ATGL), and lipophagy. Each of these processes requires access to the LD surface to initiate LD TAG catabolism. Ablation of perilipin 2 (PLIN2), the most abundant lipid droplet-associated protein in steatotic liver, protects mice from diet-induced NAFLD. However, the mechanisms underlaying this protection are unclear. We tested the contributions of ATGL and lipophagy mediated lipolysis to reduced hepatic TAG in mice with liver-specific PLIN2 deficiency (PLIN2LKO) fed a Western-type diet for 12 weeks. We observed enhanced autophagy in the absence of PLIN2, as determined by ex vivo p62 flux, as well as increased p62- and LC3-positive autophagic vesicles in PLIN2LKO livers and isolated primary hepatocytes. Increased levels of autophagy correlated with significant increases in cellular fatty acid (FA) oxidation in PLIN2LKO hepatocytes. We observed that inhibition of either autophagy or ATGL blunted the increased FA oxidation in PLIN2LKO hepatocytes. Additionally, combined inhibition of ATGL and autophagy reduced FA oxidation to the same extent as treatment with either inhibitor alone. In sum, these studies show that protection against NAFLD in the absence of hepatic PLIN2 is driven by the integrated actions of both ATGL and lipophagy.
Anti-Inflammatory Activity of Calendula officinalis L. Flower Extract
Authors: Silva Diva , Ferreira Marta Salvador , Sousa-Lobo José Manuel , Cruz Maria Teresa , Almeida Isabel Filipa
Published: April 2021

The use of calendula for its lenitive properties’ dates to the XII century. This plant contains several bioactive compounds, including terpenoids, terpenes, carotenoids, flavonoids and polyunsaturated fatty acids. Calendula flower extract is used in soothing cosmetics, such as after-sun, sensitive skin and eye contour products. The anti-inflammatory properties of this ingredient were demonstrated in an animal model, but the mechanism of action is poorly understood. Therefore, our work explored the effect of a calendula flower extract on NO production, a pro-inflammatory radical produced by nitric oxide synthase (iNOS) and highly released by innate immune cells in inflammatory-related pathologies. NO production was evoked by the Toll-like receptor 4 agonist lipopolysaccharide (LPS) in macrophages, using concentrations that did not compromise cells viability. This ingredient exhibited a dose-dependent NO inhibition, reaching 50% at 147 μL/mL without cytotoxicity. Together with previous literature, these results provide experimental evidence on the anti-inflammatory properties of calendula flower extract, as well as its usefulness in cosmetics with soothing properties and adjunctive skin care in the treatment of the diseases associated with dysregulation of the NO signaling cascade.
Recent Developments in Seafood Packaging Technologies
Authors: Kontominas Michael G. , Badeka Anastasia V. , Kosma Ioanna S. , Nathanailides Cosmas I.
Published: April 2021

Seafood products are highly perishable, owing to their high water activity, close to neutral pH, and high content of unsaturated lipids and non-protein nitrogenous compounds. Thus, such products require immediate processing and/or packaging to retain their safety and quality. At the same time, consumers prefer fresh, minimally processed seafood products that maintain their initial quality properties. The present article aims to review the literature over the past decade on: (i) innovative, individual packaging technologies applied to extend the shelf life of fish and fishery products, (ii) the most common combinations of the above technologies applied as multiple hurdles to maximize the shelf life of seafood products, and (iii) the respective food packaging legislation. Packaging technologies covered include: Modified atmosphere packaging; vacuum packaging; vacuum skin packaging; active food packaging, including oxygen scavengers; carbon dioxide emitters; moisture regulators; antioxidant and antimicrobial packaging; intelligent packaging, including freshness indicators; time–temperature indicators and leakage indicators; retort pouch processing and edible films; coatings/biodegradable packaging, used individually or in combination for maximum preservation potential.
Network Representation Learning Enhanced by Partial Community Information That Is Found Using Game Theory
Authors: Sun Hanlin , Jie Wei , Loo Jonathan , Chen Liang , Wang Zhongmin , Ma Sugang , Li Gang , Zhang Shuai
Published: April 2021

Presently, data that are collected from real systems and organized as information networks are universal. Mining hidden information from these data is generally helpful to understand and benefit the corresponding systems. The challenges of analyzing such data include high computational complexity and low parallelizability because of the nature of complicated interconnected structure of their nodes. Network representation learning, also called network embedding, provides a practical and promising way to solve these issues. One of the foremost requirements of network embedding is preserving network topology properties in learned low-dimension representations. Community structure is a prominent characteristic of complex networks and thus should be well maintained. However, the difficulty lies in the fact that the properties of community structure are multivariate and complicated; therefore, it is insufficient to model community structure using a predefined model, the way that is popular in most state-of-the-art network embedding algorithms explicitly considering community structure preservation. In this paper, we introduce a multi-process parallel framework for network embedding that is enhanced by found partial community information and can preserve community properties well. We also implement the framework and propose two node embedding methods that use game theory for detecting partial community information. A series of experiments are conducted to evaluate the performance of our methods and six state-of-the-art algorithms. The results demonstrate that our methods can effectively preserve community properties of networks in their low-dimension representations. Specifically, compared to the involved baselines, our algorithms behave the best and are the runners-up on networks with high overlapping diversity and density.
High Variability in Pre-Oviposition Time Independent of Diet Available at Eclosion: A key Reproductive Trait in the Ladybird Beetle Harmonia axyridis (Coleoptera: Coccinellidae) in Its Native Range
Authors: Hatt Séverin , Osawa Naoya
Published: April 2021

While insect predators need high-quality food to reach sexual maturity and reproduce, starvation following adult eclosion may occur when prey are missing in agroecosystems. This study explores whether the type of diet available at eclosion determines the future fecundity of newly emerged adult predators. In a laboratory experiment, three different diets (i.e., flowers of Perilla frutescens (L.) Britton, eggs of Ephestia kuehniella Zeller as prey, or no food) were offered to adult females of the ladybird beetle Harmonia axyridis Pallas during their first three days after adult eclosion. On the fourth day, each female was paired with a prey-fed male and the pair was subsequently fed with prey. Diet at eclosion did not affect pre-oviposition time, the number of eggs oviposited daily, or the viability of egg batches. High variability in pre-oviposition time among females was observed for all diets. Significant negative linear relationships were found between pre-oviposition time and both the number of eggs oviposited daily and the viability of egg batches. This study clarifies that the food readily available at adult eclosion does not affect the capacity of H. axyridis to reproduce, provided that adults find prey within a few days. More generally, it shows that the reproductive traits of H. axyridis allow this generalist predator to be highly adapted to heterogenous environments in its native range. It is concluded that the variety of habitats offered by diversified agroecosystems may highly benefit the ladybird beetle H. axyridis, and potentially enhance its ability to biologically regulate crop pests.