bluuebunny/crossref_metadata_2025 huggingface dataset:
Umbilical Cord–Derived Stem Cells for the Treatment of Knee Osteoarthritis: A Systematic Review
Authors: Dhillon Jaydeep , Kraeutler Matthew J. , Belk J. Wilson , Scillia Anthony J.
Published: July 2022

Background:

The use of mesenchymal stem cells (MSCs) for the treatment of knee osteoarthritis (OA) has gained recent interest in the orthopaedics community.

Purpose:

To review the literature to evaluate the efficacy of umbilical cord–derived MSCs in the treatment of OA of the knee joint.

Study Design:

Systematic review; Level of evidence, 4.

Methods:

We searched the PubMed, Cochrane Library, and Embase databases to identify studies with evidence levels from 1 to 4 that evaluated the clinical efficacy of human umbilical cord–derived MSC (hUC-MSC) injections for knee OA. The search phrase used was “umbilical cord knee osteoarthritis.” In the studies reviewed, patients were assessed based on the macroscopic International Cartilage Regeneration & Joint Preservation Society (ICRS) score, Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), visual analog scale (VAS) for pain, and the subjective International Knee Documentation Committee (IKDC) score.

Results:

A total of 7 studies met inclusion criteria, including 385 patients undergoing injection of hUC-MSCs (mean age, 59.7 years). The mean follow-up was 23.4 months. Weighted averages of the WOMAC, macroscopic ICRS, subjective IKDC, and VAS scores all showed improvement from before to after treatment. No severe adverse reactions were recorded.

Conclusion:

Patients undergoing treatment of knee OA with hUC-MSCs might be expected to experience improvements in clinical outcomes. Additional high-quality randomized studies are needed to better determine the efficacy of hUC-MSC for the treatment of knee OA.
Effect of Pitching Restrictions and Mound Distance on Youth Baseball Pitch Counts
Authors: Mair Hailey , Kenney Nicholas A. , Uhl Timothy L. , Ullery L. Robert , Hosey Robert G.
Published: July 2022

Background:

Studies have shown that higher pitch counts are directly related to a greater incidence of elbow and shoulder pain among youth baseball pitchers.

Purpose/Hypothesis:

The purpose of this study was to examine the effect of different pitching restriction rules on the number of pitches thrown in youth baseball leagues. We hypothesized that more pitches would be thrown in leagues with inning restrictions versus leagues with pitch count restrictions as well as in leagues with a longer mound distance (from pitching mound to home plate).

Study Design:

Cohort study; Level of evidence, 2.

Methods:

Pitch count data were collected for 2 consecutive years over a 10-week season from 3 different leagues of 9- to 12-year-old baseball players in a single city. The Eastern league had a pitch count restriction and 46-ft (14.02-m) mound distance. The Southeastern and South leagues’ pitching restrictions were based on innings per week. The Southeastern league had a 50-ft (15.24-m) mound distance, while the South league had a 46-ft mound distance. Comparisons of total seasonal pitches thrown were made of the 3 highest-volume pitchers on each team. League averages for each value were then compared utilizing analysis of variance with Bonferroni post hoc analysis. The number of pitchers in each league who threw >600 pitches per season was compared using the chi-square test.

Results:

No significant difference in seasonal pitch counts or innings pitched was noted between the Eastern and South leagues, which differed only in their pitching restrictions. The Southeastern league, with a longer mound distance, was found to have higher seasonal pitch counts per thrower (598 ± 195 pitches) than the South league (463 ± 198 pitches) for the 3 highest-volume throwers for each team ( P = .004). The Southeastern league also had a significantly larger number of pitchers who threw >600 pitches per season (33 vs 20 for Eastern and 13 for South; P = .009).

Conclusion:

There was no significant difference in seasonal pitch counts when the leagues in this study differed based on pitching restrictions. However, the league with a greater mound distance (Southeastern) had higher seasonal pitch counts for the highest-volume throwers. Pitching restrictions based on pitch counts, as opposed to innings, may be advisable.
Boon or bane? Open society and polarisation
Authors: Royer Christof
Published: July 2022

Is polarisation a fundamental threat to the open society? Are the divisions that run through societies and separate them into two (or more) more or less hostile groups problems to be solved? Or are they the corollaries of a vibrant democratic system that might legitimately be called an ‘open society’? These are the questions I seek to explore in this contribution to the special issue. My argument unfolds through a reinterpretation of Karl Popper’s conception of open society as a democratic idea, characterised by an appreciation of genuine human plurality and diversity that make ‘critical encounters with the other side’ possible (and desirable); this conception of open society also recognises the progressive potential of social and political conflicts. For that reason, political polarisation cannot be regarded as a lethal threat to open societies. By contrast, ‘belief polarisation’, with its Manichean orientation and anti-political tendencies, is a much more serious threat. It follows that advocates of open society should avoid the temptation to solve the ‘problem’ of political polarisation – they should accept it as the price to be paid for the ‘imperfect ideal’ of open society. However, they should take steps to reduce belief polarisation through the active creation of spaces of critical encounters with the other side.

The overarching aim of the article, then, is to make a contribution to both the literature on open society and polarisation. To that end, I will bring the concept of open society and the phenomenon of polarisation into a relationship of reciprocal elucidation: through the engagement with open society, I will shine some light on polarisation, and through the analysis of polarisation, I will put flesh on the concept of open society.
Toward Transdisciplinarity: Constructing Meaning Where Disciplines Intersect, Combine, and Shift
Authors: Radakovic Nenad , O’Byrne W. Ian , Negreiros Melissa , Hunter-Doniger Tracey , Pears Emily , Littlejohn Cymone
Published: July 2022

Although literacy research has traditionally focused on content area and disciplinary literacies, in this study we argue for the importance of transdisciplinarity. We employed a participatory action research design to examine pre-service teachers’ (PST) planning of a transdisciplinary math and music lesson to understand how conceptual framing of pedagogy and instructional practice can be shifted from content literacies to transdisciplinarity. Our area of inquiry focused on how PSTs’ understanding of transdisciplinarity shifted as they planned, taught, and reflected on instruction and how these shifts were supported by others. We documented evidence of how the lesson progressed from disciplinary thinking to interdisciplinary, multidisciplinary, and transdisciplinary thinking throughout the planning process. We also documented how the process of planning, teaching, coaching, and reflecting provided PSTs with an opportunity to better understand the connections between transdisciplinarity and pedagogy.
The leading role of cities in public and private discourses on urban climate governance
Authors: Díaz-Pont Joana
Published: July 2022

The paper analyses how the discourse on climate governance is re-worked in the urban context. It does so in order to better understand the meaning and possible impact of a growing rhetoric that proclaims the leadership in terms of climate governance of cities that are transitioning towards sustainable futures. By using an approach that employs critical discourse analysis, it examines the institutional climate governance discourse issued by public and private actors in the city of Barcelona, and compares it with the locally-contextualised discourses issued by these same actors during the Car-Free Day campaigns in 2005, 2012 and 2015. The objective is to analyse if private and public discourses internalise and take on the city’s leadership discourse and transfer it to urban climate governance. The comparative examination of texts released by public and private actors points to the discursive strategies of climate governance at the city level taking on meanings that are more descriptive than normative. The paper claims that this has implications for expectations regarding the city’s leadership in transitions towards sustainable futures.
Decreased Brain Ventricular Volume in Psychiatric Inpatients with Serotonin Reuptake Inhibitor Treatment
Authors: Bolin PK , Gosnell SN , Brandel-Ankrapp K , Srinivasan N , Castellanos A , Salas R
Published: July 2022

Background

Brain ventricles have been reported to be enlarged in several neuropsychiatric disorders and in aging. Whether human cerebral ventricular volume can decrease over time with psychiatric treatment is not well-studied. The aim of this study was to examine whether inpatients taking serotonin reuptake inhibitors (SRI) exhibited reductions in cerebral ventricular volume.

Methods

Psychiatric inpatients, diagnosed mainly with depression, substance use, anxiety, and personality disorders, underwent two imaging sessions (Time 1 and Time 2, approximately 4 weeks apart). FreeSurfer was used to quantify volumetric features of the brain, and ANOVA was used to analyze ventricular volume differences between Time 1 and Time 2. Inpatients’ brain ventricle volumes were normalized by dividing by estimated total intracranial volume (eTIV). Clinical features such as depression and anxiety levels were collected at Time 1, Time 1.5 (approximately 2 weeks apart), and Time 2.

Results

Inpatients consistently taking SRIs (SRI + , n = 44) showed statistically significant reductions of brain ventricular volumes particularly for their left and right lateral ventricular volumes. Reductions in their third ventricular volume were close to significance ( p =  0.063). The inpatients that did not take SRIs (SRI-, n = 25) showed no statistically significant changes in brain ventricular volumes. The SRI + group also exhibited similar brain structural features to the healthy control group based on the 90% confidence interval comparsions on brain ventricular volume parameters, whereas the SRI- group still exhibited relatively enlarged brain ventricular volumes after treatment.

Conclusions

SRI treatment was associated with decreased brain ventricle volume over treatment.
Making global oceans governance in/visible with Smart Earth: The case of Global Fishing Watch
Authors: Drakopulos Lauren , Silver Jennifer J. , Nost Eric , Gray Noella , Hawkins Roberta
Published: July 2022

The number and variety of technologies used for environmental surveillance is expanding rapidly, making constant data collection and near ‘real time’ analyses possible. ‘Smart Earth’ describes networked infrastructures comprised of devices and equipment and signals to the human dimensions inherent to developing, deploying and putting technology and large datasets to use. In this paper, we situate Smart Earth in terms of technological products and human practices and consider the relationship between Smart Earth and global environmental governance. Specifically, we review emerging literature and present a case study of an organization founded by environmental non-profit, SkyTruth, tech industry behemoth, Google and marine conservation NGO, Oceana. Called ‘Global Fishing Watch’ (GFW), this organization builds geospatial datasets, hosts an online mapping platform where anyone with internet access can surveil various types of ocean-going vessels and shares data and map products with scientists and practitioners. Two critical points emerge through the case. First, we show that GFW expands its surveillance capacity by pursuing ‘data sharing’ partnerships with sovereign states, many in the Global South. Second, the maps and datasets produced by GFW link vessels to a ‘flag state’ while the firms, subsidiaries and financiers that may own and/or operate these vessels remain obscure – and hence so too does the political economy of oceans fisheries. GFW maps and datasets offer new approaches to tracking fishing and are advancing fisheries science. At the same time, they rely on and are only legible through hegemonic geopolitical and political–economic orders deeply implicated in industrial (over)fishing. The norms and domains of global environmental governance are expanding, but Smart Earth ‘solutions’ risk leaving the structural drivers of environmental change unaddressed.
Diabetes Distress in Emerging Adulthood: Content Validity of the Problem Areas in Diabetes—Emerging Adult Version (PAID-EA) Using Qualitative Analysis
Authors: Wentzell Katherine , Vitale Rebecca , Laffel Lori
Published: July 2022

Purpose:

The purpose of this study was to explore the emotional work of diabetes during emerging adulthood and to explicate the validity of a newly developed measure of diabetes distress (DD) for use with emerging adults living with type 1 diabetes mellitus (T1DM), the Problem Areas in Diabetes—Emerging Adult version (PAID-EA).

Methods:

Young people ages 18 to 30 with T1DM were recruited online to complete a cross-sectional survey including measures of DD, depressive symptomology, and the PAID-EA. To evaluate content validity, 2 open-ended questions asked what was the most significant emotion or worry discussed in the survey items and what feelings were missed in those items. Responses were analyzed using directed qualitative content analysis.

Results:

A total of 254 (87%) participants responded to at least 1 of the 2 open-ended questions. Three themes and 1 subtheme were identified: (1) fear of the future with the subtheme of worry about the cost of diabetes, (2) acute worries about living with diabetes, and (3) challenges with finding support. More PAID-EA items corresponded with these themes than items on the original Problem Areas in Diabetes or Center for Epidemiologic Studies Depression Scale, supporting the validity of the PAID-EA and clarifying the developmental-stage-specific aspects of DD.

Conclusions:

Emerging adulthood is a period in which the future should hold infinite possibility, but young people with T1DM describe a staggering fear of the future with markedly limited possibilities, supporting the need to measure the developmental-stage-specific experience of DD as captured on the PAID-EA.
Distal Biceps Tendon Repair Using a Unicortical Button
Authors: Barrazueta Gustavo , Awan Hisham , Cvetanovich Gregory
Published: July 2022

Background:

Distal biceps tendon ruptures occur most commonly in the dominant arm of men in their 4th through 6th decades of life. These injuries lead to a 30% reduction in flexion strength and 40% reduction in supination strength. Numerous methods exist for fixation of the distal biceps tendon, including bicortical endobutton, transosseous tunnels, suture anchors, and interference screws. We review the technique for using a unicortical button fixation method.

Indications:

Indications for surgical fixation of the distal biceps tendon include complete tendon ruptures, where a loss of elbow flexion and forearm supination strength of 30% and 40%, respectively, is not desired and partial tendon ruptures that have failed nonoperative treatment. Typically, nonoperative treatment of partial tendon ruptures is tried for a minimum of 3 months.

Technique Description:

We present the technique of repairing a distal biceps tendon rupture using a unicortical button. A 1-incision technique is utilized through a transverse incision approximately 3-4 cm distal to the elbow flexion crease. Onlay fixation of the distal biceps tendon to the radial tuberosity is achieved with a unicortical, or intramedullary, button. The postoperative protocol varies with surgeon but is similar to other fixation techniques.

Results:

Outcomes for primary repair of the distal biceps tendon are promising in the literature with both patient-reported outcomes and objective data showing good to excellent results. Bicortical button fixation has traditionally shown to be stiffer with a higher load to failure than other fixation techniques, including suture anchors, transosseous tunnels, and interference screws. The unicortical button has been shown to be statistically equivalent to the bicortical button, with similar cyclic loading and load to failure values.

Discussion/Conclusion:

The unicortical button technique for distal biceps repairs has the benefit of using a small footprint in the radial tuberosity, potentially decreasing the risk of heterotopic ossification, providing a safer avenue of obtaining anatomic placement and trajectory of repair, and decreasing the risk of posterior interosseous nerve injury. The unicortical button has been shown to have a similar strength profile to the bicortical button, which is higher than all other fixation techniques previously described in the literature.
The novel transcriptomic signature of angiogenesis predicts clinical outcome, tumor microenvironment and treatment response for prostate adenocarcinoma
Authors: Gu Cheng-Yuan , Dai Bo , Zhu Yao , Lin Guo-Wen , Wang Hong-Kai , Ye Ding-Wei , Qin Xiao-Jian
Published: July 2022

Angiogenesis plays the critical roles in promoting tumor progression, aggressiveness, and metastasis. Although few studies have revealed some angiogenesis-related genes (ARGs) could serve as prognosis-related biomarkers for the prostate cancer (PCa), the integrated role of ARGs has not been systematically studied. The RNA-sequencing data and clinical information of prostate adenocarcinoma (PRAD) were downloaded from The Cancer Genome Atlas (TCGA) as discovery dataset. Twenty-three ARGs in total were identified to be correlated with prognosis of PRAD by the univariate Cox regression analysis, and a 19-ARG signature was further developed with significant correlation with the disease-free survival (DFS) of PRAD by the least absolute shrinkage and selection operator (LASSO) Cox regression with tenfold cross-validation. The signature stratified PRAD patients into high- and low-ARGs signature score groups, and those with high ARGs signature score were associated with significantly poorer outcomes (median DFS: 62.71 months vs unreached, p < 0.0001). The predicting ability of ARGs signature was subsequently validated in two independent cohorts of GSE40272 & PRAD_MSKCC. Notably, the 19-ARG signature outperformed the typical clinical features or each involved ARG in predicting the DFS of PRAD. Furthermore, a prognostic nomogram was constructed with three independent prognostic factors, including the ARGs signature, T stage and Gleason score. The predicted results from the nomogram (C-index = 0.799, 95%CI = 0.744–0.854) matched well with the observed outcomes, which was verified by the calibration curves. The values of area under receiver operating characteristic curve (AUC) for DFS at 1-, 3-, 5-year for the nomogram were 0.82, 0.83, and 0.83, respectively, indicating the performance of nomogram model is of reasonably high accuracy and robustness. Moreover, functional enrichment analysis demonstrated the potential targets of E2F targets, G2M checkpoint pathways, and cell cycle pathways to suppress the PRAD progression. Of note, the high-risk PRAD patients were more sensitive to immune therapies, but Treg might hinder benefits from immunotherapies. Additionally, this established tool also could predict response to neoadjuvant androgen deprivation therapy (ADT) and some chemotherapy drugs, such as cisplatin, paclitaxel, and docetaxel, etc. The novel ARGs signature, with prognostic significance, can further promote the application of targeted therapies in different stratifications of PCa patients.
m6A-modified circRNAs: detections, mechanisms, and prospects in cancers
Authors: Qin Shiyi , Zhang Qi , Xu Yanhua , Ma Shuo , Wang Tianyi , Huang Yuejiao , Ju Shaoqing
Published: July 2022

Circular RNAs (circRNAs) have become a research hotspot in recent years with their universality, diversity, stability, conservativeness, and spatiotemporal specificity. N6-methyladenosine (m6A), the most abundant modification in the eukaryotic cells, is engaged in the pathophysiological processes of various diseases. An increasing amount of evidence has suggested that m6A modification is common in circRNAs and is associated with their biological functions. This review summarizes the effects of m6A modification on circRNAs and their regulation mechanisms in cancers, providing some suggestions of m6A-modified circRNAs in cancer therapy.
Uncertainty Analysis and Optimization of Quasi-Zero Stiffness Air Suspension Based on Polynomial Chaos Method
Authors: Xu Xing , Liu Huan , Jiang Xinwei , Atindana Akolbire Vincent
Published: July 2022

To improve the vibration isolation performance of suspensions, various new structural forms of suspensions have been proposed. However, there is uncertainty in these new structure suspensions, so the deterministic research cannot reflect the performance of the suspension under actual operating conditions. In this paper, a quasi-zero stiffness isolator is used in automotive suspensions to form a new suspension−quasi-zero stiffness air suspension (QZSAS). Due to the strong nonlinearity and structural complexity of quasi-zero stiffness suspensions, changes in structural parameters may cause dramatic changes in suspension performance, so it is of practical importance to study the effect of structural parameter uncertainty on the suspension performance. In order to solve this problem, three suspension structural parameters d0, L0 and Pc0 are selected as random variables, and the polynomial chaos expansion (PCE) theory is used to solve the suspension performance parameters. The sensitivity of the performance parameters to different structural parameters was discussed and analyzed in the frequency domain. Furthermore, a multi-objective optimization of the structural parameters d0, L0 and Pc0 of QZSAS was performed with the mean and variance of the root-mean-square (RMS) acceleration values as the optimization objectives. The optimization results show that there is an improvement of about 8%−10% in the mean value and about 40%−55% in the standard deviation of acceleration (RMS) values. This paper verifies the feasibility of the PCE method for solving the uncertainty problem of complex nonlinear systems, which provide a reference for the future structural design and optimization of such suspension systems.
METTL3 mediates Ang-II-induced cardiac hypertrophy through accelerating pri-miR-221/222 maturation in an m6A-dependent manner
Authors: Zhang Rui , Qu Yangyang , Ji Zhenjun , Hao Chunshu , Su Yamin , Yao Yuyu , Zuo Wenjie , Chen Xi , Yang Mingming , Ma Genshan
Published: July 2022

Background

METTL3 is the core catalytic enzyme in m6A and is involved in a variety of cardiovascular diseases. However, whether and how METTL3 plays a role during angiotensin II (Ang-II)-induced myocardial hypertrophy is still unknown.

Methods

Neonatal rat cardiomyocytes (NRCMs) and C57BL/6J mice were treated with Ang-II to induce myocardial hypertrophy. qRT-PCR and western blots were used to detect the expression of RNAs and proteins. Gene function was verified by knockdown and/or overexpression, respectively. Luciferase and RNA immunoprecipitation (RIP) assays were used to verify interactions among multiple genes. Wheat germ agglutinin (WGA), hematoxylin and eosin (H&E), and immunofluorescence were used to examine myocardial size. m6A methylation was detected by a colorimetric kit.

Results

METTL3 and miR-221/222 expression and m6A levels were significantly increased in response to Ang-II stimulation. Knockdown of METTL3 or miR-221/222 could completely abolish the ability of NRCMs to undergo hypertrophy. The expression of miR-221/222 was positively regulated by METTL3, and the levels of pri-miR-221/222 that bind to DGCR8 or form m6A methylation were promoted by METTL3 in NRCMs. The effect of METTL3 knockdown on hypertrophy was antagonized by miR-221/222 overexpression. Mechanically, Wnt/β-catenin signaling was activated during hypertrophy and restrained by METTL3 or miR-221/222 inhibition. The Wnt/β-catenin antagonist DKK2 was directly targeted by miR-221/222, and the effect of miR-221/222 inhibitor on Wnt/β-catenin was abolished after inhibition of DKK2. Finally, AAV9-mediated cardiac METTL3 knockdown was able to attenuate Ang-II-induced cardiac hypertrophy in mouse model.

Conclusions

Our findings suggest that METTL3 positively modulates the pri-miR221/222 maturation process in an m6A-dependent manner and subsequently activates Wnt/β-catenin signaling by inhibiting DKK2, thus promoting Ang-II-induced cardiac hypertrophy. AAV9-mediated cardiac METTL3 knockdown could be a therapeutic for pathological myocardial hypertrophy.
Toward a standard formal semantic representation of the model card report
Authors: Amith Muhammad Tuan , Cui Licong , Zhi Degui , Roberts Kirk , Jiang Xiaoqian , Li Fang , Yu Evan , Tao Cui
Published: July 2022

Background

Model card reports aim to provide informative and transparent description of machine learning models to stakeholders. This report document is of interest to the National Institutes of Health’s Bridge2AI initiative to address the FAIR challenges with artificial intelligence-based machine learning models for biomedical research. We present our early undertaking in developing an ontology for capturing the conceptual-level information embedded in model card reports.

Results

Sourcing from existing ontologies and developing the core framework, we generated the Model Card Report Ontology. Our development efforts yielded an OWL2-based artifact that represents and formalizes model card report information. The current release of this ontology utilizes standard concepts and properties from OBO Foundry ontologies. Also, the software reasoner indicated no logical inconsistencies with the ontology. With sample model cards of machine learning models for bioinformatics research (HIV social networks and adverse outcome prediction for stent implantation), we showed the coverage and usefulness of our model in transforming static model card reports to a computable format for machine-based processing.

Conclusions

The benefit of our work is that it utilizes expansive and standard terminologies and scientific rigor promoted by biomedical ontologists, as well as, generating an avenue to make model cards machine-readable using semantic web technology. Our future goal is to assess the veracity of our model and later expand the model to include additional concepts to address terminological gaps. We discuss tools and software that will utilize our ontology for potential application services.
MicroRNA-145-5p modulates Krüppel-like factor 5 and inhibits cell proliferation, migration, and invasion in nasopharyngeal carcinoma
Authors: Yuan Chien-Han , Hsu Wei-Chi , Huang A.-Mei , Yuan Ben-Chih , Chen I.-Hung , Hsu Chia-An , Chen Rong-Feng , Chu Yih-Min , Lin Hui-Hui , Ke Hung-Lung
Published: July 2022

Background

In several human cancers, Krüppel-like factor 5 (KLF5), a zinc finger transcription factor, can contribute to both tumor progression or suppression; however, the precise role of KLF5 in nasopharyngeal carcinoma (NPC) remains poorly understood. In this study, the association between KLF5 and microRNA-145-5p (miR-145-5p) in NPC cells was elucidated.

Results

Our results showed that KLF5 expression was up-regulated in NPC group compared to normal group. We found that KLF5 exhibited an oncogenic role in NPC cells. The upregulation of miR-145-5p inhibited the proliferation, migration, and invasion of NPC cells. It was observed that miR-145-5p could down-regulate the mRNA and protein expression of KLF5 in NPC cell lines. Additionally, the activity of focal adhesion kinase (FAK), a migration marker, was regulated by miR-145-5p and KLF5 in NPC cells.

Conclusions

The results of this study indicated that miR-145-5p could repress the proliferation, migration, and invasion of NPC cells via KLF5/FAK regulation, and could be a potential therapeutic target for patients with NPC.
Does group-based trajectory modeling estimate spurious trajectories?
Authors: Mésidor Miceline , Rousseau Marie-Claude , O’Loughlin Jennifer , Sylvestre Marie-Pierre
Published: July 2022

Background

Group-based trajectory modelling (GBTM) is increasingly used to identify subgroups of individuals with similar patterns. In this paper, we use simulated and real-life data to illustrate that GBTM is susceptible to generating spurious findings in some circumstances.

Methods

Six plausible scenarios, two of which mimicked published analyses, were simulated. Models with 1 to 10 trajectory subgroups were estimated and the model that minimized the Bayes criterion was selected. For each scenario, we assessed whether the method identified the correct number of trajectories, the correct shapes of the trajectories, and the mean number of participants of each trajectory subgroup. The performance of the average posterior probabilities, relative entropy and mismatch criteria to assess classification adequacy were compared.

Results

Among the six scenarios, the correct number of trajectories was identified in two, the correct shapes in four and the mean number of participants of each trajectory subgroup in only one. Relative entropy and mismatch outperformed the average posterior probability in detecting spurious trajectories.

Conclusion

Researchers should be aware that GBTM can generate spurious findings, especially when the average posterior probability is used as the sole criterion to evaluate model fit. Several model adequacy criteria should be used to assess classification adequacy.
Patient perspectives on the management of COPD and Type 2 Diabetes in general practice: an interview study
Authors: Lee Kim , Titlestad Signe Beck , Nørgaard Birgitte , Bentzen Niels , Søndergaard Jens , Marcussen Michael
Published: July 2022

Background

The Danish healthcare system has undergone fundamental organisational changes. In recent years, treatment of most patients with chronic obstructive pulmonary disease (COPD) and type 2 diabetes (T2D) in Denmark has been transferred from specialised hospitals to general practices, and only the most complicated cases are treated at hospital outpatients clinics or are admitted. This transfer aimed to reduce costs without compromising quality of care and ensure that the treatment was managed by general practitioners (GPs) who had personal knowledge of the patient. In this paper, we explore patients’ perceptions of the quality of care provided by their GPs.

Methods

A qualitative research study was conducted with semi-structured interviews of 24 informants; nine were diagnosed with COPD and 15 were diagnosed with T2D. Snowball sampling was used for recruitment. Data were analysed using systematic text condensation.

Results

The interviews revealed four main themes:

1) The informants perceived the quality of their treatment in general practice to be high due to their personal relationship with their GPs.

2) The informants valued their GP’s knowledge about them, their lives, and their illnesses.

3) The informants expressed a high degree of satisfaction with the quality of care received in general practice.

4) The informants expressed that geographical distance to the general practice was of minor importance to them.

Conclusion

The patients perceived that the quality of the care and treatment they received were high following the transfer of COPD and T2D treatment to general practice. A strong, trusting relationship between the GP and the patient and the increased availability of the GP both contributed to their satisfaction with the GPs’ services.
Translation and psychometric validation of the Arabic version of Summary of the Diabetes Self-Care Activities (SDSCA) among pregnant women with gestational diabetes
Authors: Al Hashmi I. , Al-Noumani H. , Alaloul F. , Murthi S. , Khalaf A.
Published: July 2022

Background and purpose

There is a lack of validated tools to assess adherence to gestational diabetes (GDM) management plan among women with GDM. This study aimed to translate the Summary of Diabetes Self-Care Activity (SDSCA) into Arabic, culturally adapt it, and test its psychometric properties among women with GDM.

Methods

A multiphase study was used to translate and evaluate the psychometric properties of the Arabic version of SDSCA using the following steps: (1) cultural and linguistic validation; (2) content and face validity testing; (3) construct validity testing; and (4) internal validity testing. Nineghty pregnant women with GDM were recruited to meet the purpose of this study.

Results

The Arabic version of the adapted SDSCA tool revealed adequate content validity, satisfactory internal consistency (Cronbach’s alpha = 0.74), and test-retest reliability (Pearson correlation coefficient = .67). Exploratory factor analysis revealed three factors that fit data satisfactory: diet, exercise, and blood glucose monitoring.

Conclusions

This study showed that the adapted Arabic SDSCA tool is an easy, valid, and reliable tool to assess pregnant women’s adherence to GDM management plan.
Anesthetic propofol enhances cisplatin-sensitivity of non-small cell lung cancer cells through N6-methyladenosine-dependently regulating the miR-486-5p/RAP1-NF-κB axis
Authors: Ling Quan , Wu Shaoyong , Liao Xiaozu , Liu Chiyi , Chen Yong
Published: July 2022

Background

Drug resistance is a considerable challenge for chemotherapy in non-small cell lung cancer (NSCLC). Propofol, a commonly used intravenous anesthetics, has been reported to suppress the malignancy of various cancers. However, the effects of propofol on cisplatin (DDP) sensitivity in NSCLC and its molecular mechanisms have not been clearly clarified yet, and the present study aimed to resolve this problem.

Methods

NSCLC cells were co-treated with propofol and DDP, Cell Counting kit-8 assay, colony formation assay and flow cytometry were conducted to test the role of propofol in regulating DDP-resistance in NSCLC. Next, through conducting quantitative real-time polymerase chain reaction, dual-luciferase gene reporter system and western blot, the responsible molecular axis in propofol regulating the DDP sensitivity in NSCLC was uncovered, and the function verification experiments were performed by transfection with the inhibitors or small interfering RNAs of those molecules.

Results

Propofol suppressed cell viability, colony formation ability, tumorigenesis, and promoted cell apoptosis to enhance DDP-sensitivity in NSCLC in vitro and in vivo. Propofol increased miR-486-5p level in NSCLC cells and xenograft tumors tissues in a N6-methyladenosine (m6A)-dependent manner, thus inactivating the Ras-associated protein1 (RAP1)-NF-kappaB (NF-κB) axis. Propofol regulated the miR-486-5p/RAP1-NF-κB axis to improve DDP-sensitivity in NSCLC.

Conclusions

Taken together, this study firstly investigates the detailed molecular mechanisms by which propofol enhanced DDP-sensitivity in NSCLC cells, and a novel m6A-dependent miR-486-5p/RAP1-NF-κB axis is identified to be closely associated with the process.
Comparative analysis of a presbyopia-correcting intraocular lens that combines extended depth-of-focus and bifocal profiles with a standard monofocal intraocular lens
Authors: Shin Da Eun , Lee Hun , Koh Kyungmin
Published: July 2022

Background

Recently, a new presbyopia-correcting intraocular lens (IOL) that combines extended depth-of-focus and bifocal profiles (ZFR00: Tecnis® Synergy®, Johnson & Johnson Vision, Santa Ana, CA, USA) has been established and several studies have been reported. We attempted to compare the performance with a standard IOL (ZCB00: Tecnis® monofocal, Johnson & Johnson Vision, Santa Ana, CA, USA) manufactured using the same material from the same company, which has been extensively used worldwide.

Methods

The medical records of patients undergoing cataract surgery with ZCB00 or ZFR00 implantation between March 2021 and September 2021 and with available 3-month visit data were reviewed. Uncorrected near, intermediate, and distance visual acuity (VA), corrected distance VA, and optical quality were the main outcome measures.

Results

This study included forty-six patients (64 eyes), with twenty-one patients (32 eyes) implanted with ZCB00 and twenty-five patients (32 eyes) implanted with ZFR00. The average age of the patients was 66.0 ± 10.1 (range: 40 to 82) and 65.1 ± 4.7 (range: 59 to 77) years in the ZCB00 and ZFR00 groups, respectively. The preoperative characteristics did not differ significantly between the two groups. Compared to the ZCB00 group, the ZFR00 group demonstrated significantly superior intermediate and near VA (p < 0.001) at 3 months postoperatively. The ZFR00 group showed significantly lower objective measured optical quality than that in the ZCB00 group.

Conclusions

The ZFR00 exhibited a continuous range of vision and a smooth defocus curve, while the ZCB00 provided superior objective optical quality.
Precocious puberty or growth hormone deficiency as initial presentation in Mayer-Rokitansky-kuster-Hauser syndrome: a clinical report of 5 cases
Authors: Ai Zhuanzhuan , Zhu Xiaoyun , Chen Hong , Chen Ruimin
Published: July 2022

Background

We report five patients with Mayer-Rokitansky-Küster-Hauser syndrome (MRKHS), four of whom presented with precocious puberty and one with growth hormone deficiency (GHD. Our five children add to the growing endocrine data base of MRKHS.

Case presentation

We retrospectively reviewed clinical data of 5 MRKHS patients from 2017 to 2020. The clinical features, hormonal profiles, radiological imaging and genetic analyses were collated. The age range of the 5 patients at diagnosis was 6.7–9.1 years. Four presented with premature thelarche, and one presented with short stature. External genitalia were normal in all patients. Gonadotropin-releasing hormone stimulation tests for the 5 patients revealed peak luteinizing hormone and follicular stimulating hormone levels of 3.57, 6.24, 11.5, 4.44 and 4.97 IU/L and 9.41, 16.7, 13.8, 14.2 and 10.3 mIU/mL, respectively. Growth hormone stimulation for one patient with short stature was consistent with GHD with a peak level of GH was 7.30 ng/mL. Imaging disclosed advanced bone age in four patients and no skeletal abnormalities in any of the patients. Ultrasonography of the abdomen revealed bilateral polycystic kidneys in one patient. Pelvic magnetic resonance imaging confirmed no uterus in five patients. All of the patients had a normal karyotype (46, XX). In one patient, whole-exome sequencing detected a deletion of 17q12(chr17:36,046,434–36,105,050, hg19) encompassing the HNF1B gene.

Conclusions

We report the unusual co-occurrence of precocious puberty and GHD in patients with MRKHS, highlighting that abnormal puberty and growth development may represent initial unexplained manifestations. Whether the deletion of 17q 22 begat GHD is unclear.
Household access to basic drinking water, sanitation and hygiene facilities: secondary analysis of data from the demographic and health survey V, 2017–2018
Authors: Gaffan Nicolas , Kpozèhouen Alphonse , Dégbey Cyriaque , Glèlè Ahanhanzo Yolaine , Glèlè Kakaï Romain , Salamon Roger
Published: July 2022

Background

In Benin, access to water, sanitation and hygiene (WASH) remains an issue. This study aims to provide an overview of household access to basic WASH services based on nationally representative data.

Method

Secondary analyses were run using the ‘HOUSEHOLD’ dataset of the fifth Demographic and Health Survey 2017–2018. The dependent variables were household access to individual and combined basic WASH services. The characteristics of the household head and those related to the composition, wealth and environment of the household were independent variables. After a descriptive analysis of all study variables, multivariate logistic regression was performed to identify predictors of outcome variables.

Results

The study included 14,156 households. Of these, 63.98% (95% CI = 61.63–66.26), 13.28% (95% CI = 12.10–14.57) and 10.11% (95% CI = 9.19–11.11) had access to individual basic water, sanitation and hygiene facilities, respectively. Also, 3% (95% CI = 2.53–3.56) of households had access to combined basic WASH services. Overall, the richest households and few, and those headed by people aged 30 and over, female and with higher levels of education, were the most likely to have access to individual and combined basic WASH services. In addition, disparities based on the department of residence were observed.

Conclusion

The authors suggest a multifactorial approach that addresses the identified determinants.
Learning about COVID-19: sources of information, public trust, and contact tracing during the pandemic
Authors: Amara Philip S. , Platt Jodyn E. , Raj Minakshi , Nong Paige
Published: July 2022

Objective

To assess the association between public attitudes, beliefs, and information seeking about the COVID-19 pandemic and willingness to participate in contact tracing in Michigan.

Methods

Using data from the quarterly Michigan State of the State survey conducted in May 2020 (n = 1000), we conducted multiple regression analyses to identify factors associated with willingness to participate in COVID-19 contact tracing efforts.

Results

Perceived threat of the pandemic to personal health (B = 0.59, p = <.00, Ref = No threat) and general trust in the health system (B = 0.17, p < 0.001), were the strongest positive predictors of willingness to participate in contact tracing. Concern about misinformation was also positively associated with willingness to participate in contact tracing (B = 0.30, p < 0.001; Ref = No concern). Trust in information from public health institutions was positively associated with willingness to participate in contact tracing, although these institutions were not necessarily the main sources of information about COVID-19.

Conclusion

Policy makers can enhance willingness to participate in public health efforts such as contact tracing during infectious disease outbreaks by helping the public appreciate the seriousness of the public health threat and communicating trustworthy information through accessible channels.
Hearing loss, sick leave, and disability pension: findings from the HUNT follow-up study
Authors: Jørgensen Astrid Ytrehus , Aarhus Lisa , Engdahl Bo , Bratsberg Bernt , Skirbekk Vegard Fykse , Mehlum Ingrid Sivesind
Published: July 2022

Background

Evidence on the association between hearing loss and sick leave or disability pension is to a great extent based on few cross-sectional studies and remains unclear. We aim to assess the associations in a long-term follow-up population study.

Methods

We used baseline data from a large population-based hearing study in Norway, the HUNT Hearing study (1996–1998). The sample included 21 754 adults (48.5% men, mean age at baseline 36.6 years). We used register data on sick leave and disability pension (1996–2011). Cox regression was used to assess the association between hearing loss at baseline (Pure tone average/PTA 0.5–4 kHz > 20 dB) and time to first physician-certified sick leave episode, as well as time to first disability pension payment.

Results

Hearing loss at baseline (yes/no) was weakly associated with time to first physician-certified sick leave episode: Hazard ratio (HR) 1.2 (95% confidence interval (CI) 1.1–1.3). Restricting the exposed group to people with both hearing loss and tinnitus, the HR was slightly increased: 1.3 (95% CI 1.1–1.6). Hearing loss in 1996–1998 was also associated with time to first received disability pension: HR 1.5 (95% CI 1.3–1.8). Stronger associations were found for disabling hearing loss (PTA > 35). Restricting the exposure to hearing loss and tinnitus, the HR was increased: 2.0 (95% CI 1.4–2.8).

Conclusions

This large population-based cohort study indicates that hearing loss is associated with increased risk of receiving disability pension, especially among younger adults and low educated workers. Hearing loss was weakly associated with sick leave.
The effect of a theory-based educational program on southern Iranian prisoners’ HIV preventive behaviors: a quasi-experimental research
Authors: Hosseini Zahra , Najafi Pirdad , Mohseni Shokrollah , Aghamolaei Teamur , Dadipoor Sara
Published: July 2022

Background

The present research explored the effect of an educational program based on the health belief model (HBM) on prisoners’ HIV preventive behaviors in the south of Iran.

Methods

The present quasi-experimental research was conducted in 2019–20 on 280 prisoners, 140 in the control group (CG) and 140 in the intervention group (IG). The sampling was simple randomized. The data were collected using a questionnaire in two parts, one exploring the demographic information and the other the HBM constructs. The final follow-up was completed 3 months after the educational intervention (8 sessions long) in November 2020.

Results

After the intervention, statistically significant between-group differences were found in the healthy behavior score and all HBM constructs except for the perceived barriers (p < 0.001). Perceived severity and susceptibility were found to be the strongest predictors of HIV preventive behaviors.

Conclusion

The educational intervention showed to positively affect the adoption of preventive behaviors mediated by the HBM constructs. To remove barriers to HIV preventive behaviors or any other healthy behavior, researchers are suggested to develop multi-level interventions (beyond the personal level) to gain better findings.
Differences in salient beliefs associated with voluntary exercise training among South Korean firefighters before and after COVID-19
Authors: Kwon Junhye , Choi Joohee , Kwon Juhyuk , Lee Chung Gun , Seo Dong-il , Song Wook , Park Jung-jun , Lee Han-joon , Kang Hyun Joo , Ahn Yeon Soon
Published: July 2022

Background

Participating in voluntary exercise training is important to meet occupational requirements as well as firefighters’ health and safety. The purpose of this study is to identify salient beliefs associated with voluntary exercise training among firefighters in the pandemic era by comparing outcomes with those from a previous elicitation study, which was carried out before the COVID-19 outbreak.

Methods

A total of 57 firefighters are recruited to participate in an elicitation study. Participants are requested to respond to six open-ended questions related to voluntary exercise training. Content analysis is used to create categories that combine similar factors in each belief. Beliefs mentioned by more than 30% of participants are used for comparison with the results of the previous research.

Results

“Improves my physical ability” (n = 44) and “cause injury” (n = 17) are identified as behavioral beliefs in the present study, whereas “makes me tired” and “takes too much time” were also elicited in Lee’s study. Normative beliefs are “family members” (n = 45) and “colleagues” (n = 27) and these results are consistent with those in Lee’s study. “Lack of time” (n = 28), “exercise facilities” (n = 19), and “COVID-19” (n = 19) are elicited as control beliefs in the present study, whereas “physical condition” (n = 21) and “exercise partners” (n = 14) were elicited as other control beliefs, and “COVID-19” was not mentioned in Lee’s study.

Conclusion

This study can contribute valuable information about salient beliefs associated with exercise training behavior among firefighters, particularly under pandemic conditions. Future researchers should develop tailored exercise training programs for firefighters based on current elicited beliefs.