bluuebunny/crossref_metadata_2025 huggingface dataset:
Photophysiological responses of bottom sea-ice algae to fjord dynamics and rapid freshening
Authors: Forgereau Zoé L. , Lange Benjamin A. , Gradinger Rolf , Assmy Philipp , Osanen Janina E. , García Laura M. , Søreide Janne E. , Granskog Mats A. , Leu Eva , Campbell Karley
Published: December 2023

Sea ice algae have a broad salinity tolerance but can experience stress during rapid decreases in salinity that occur with seasonal ice melt and during ice sample melt. This study investigated the impact of salinity on the photophysiological responses of bottom-ice algal communities from two Svalbard fjords (Tempelfjorden and Van Mijenfjorden). To further investigate the impact of salinity alone, and particularly to rapid freshening, the responses of a lab-cultured ice algal community from Van Mijenfjorden were assessed. Photophysiological responses were mainly determined via 14C-based incubations which provided photosynthesis-irradiance curves. Main findings showed that i) the bottom-ice algal community in Tempelfjorden was characterized by lower photosynthetic efficiency and chlorophyll a biomass than the Van Mijenfjorden communities, and ii) a lab-cultured ice algal community from Van Mijenfjorden dominated by pennate diatoms had significantly lower photosynthetic efficiency, maximum photosynthesis and photoacclimation index after a decrease in salinity from 33 to 10. The lower photosynthetic efficiency and chlorophyll a biomass at Tempelfjorden may be attributed to the almost two-fold lower bulk-ice salinity in Tempelfjorden compared to Van Mijenfjorden, which was likely associated with freshwater inputs from the tidewater glacier Tunabreen during sea ice formation. Other factors such as under-ice light intensities, brine volume fraction and brine nutrient concentrations likely also contributed to variability in ice algal response. Furthermore, experimental results indicated that the cultured Van Mijenfjorden community was negatively impacted by a rapid (within 4 to 24 h) reduction in salinity from 33 to 10. We further documented a significant start of recovery of these algae after 168 h. From this work, we surmise that decreases in surface water salinity, for example arising from the intensifying freshening of fjord waters, may only cause temporary changes in ice algal photoacclimation state and thus in chlorophyll a biomass. Further, this study also supports the need for salinity buffered melt of sea ice samples to reduce artificial bias in biological measurements.
Depositional evolution in response to long-term marine transgression in the northern South China Sea
Authors: Liu Entao , Luo Wei , Yan Detian , Deng Yong , Chen Si , Zhong Jialin , Jiao Yangshuo
Published: December 2023

Research on the interaction between depositional evolution process and marine transgression is critical to understanding the transform mechanism of sedimentary systems and guiding hydrocarbon exploration. The early Miocene witnessed the most significant sea-level rise since the Cenomanian, which resulted in extensive marine-influenced deposits worldwide. However, the relationship between the process of depositional evolution and long-term marine transgressions (>1 Ma) remains poorly understood. The Pearl River Mouth Basin in the South China Sea offers a comprehensive deposition record of the early Miocene marine transgression. This study employs high-quality 3D seismic, well-logging, and core data to investigate the impact of the early Miocene transgression on the evolutionary dynamics of the sedimentary system. The regional sea level exhibited a prolonged rise of at least 100 m during the deposition period of the Miocene Zhujiang Formation, corresponding to the long-term marine transgressive in the South China Sea. Throughout this marine transgression, depositional systems developed in the study area include tidal flats, fan deltas, meandering river deltas, and shallow marine shelf sand bodies. The marine transgression process resulted in a significant change in depositional system types, which can be divided into seven units from Unit 1 at the bottom to Unit 7 at the top. The predominant deposition environment transitioned from tidal flats in Units 1-3 to meandering river deltas in Units 4-5, and finally to shallow marine shelf systems in Units 6-7. In the early stage (Units 1-3), the regional uplifts hindered sea level transgression and caused erosion, leading to the development of small-scale proximal fan deltas. In the middle stage (Units 4-5), these regional uplifts submerged, and meandering river deltas dominated with sediments derived from distant extrabasinal sources. During the late stage (Units 6-7), regional sea levels reached their peak, transforming the entire basin into a shallow marine shelf system. Additionally, this marine transgression significantly influenced the distribution of hydrocarbon resources. Notably, the shallow marine shelf sand bodies in Units 6-7 warrant substantial attention for future exploration. This study outlines the complicated transitional processes within depositional systems during long-term marine transgression events, holding relevance for the global evolution of marginal sea basins.
Should acupuncture become a complementary therapy in the treatment of uterine fibroid: a systematic review and meta-analysis of randomized controlled trials
Authors: Ren Yuehan , Zhang Junning , Wu Weizhen , Yuan Yi , Wang Jiale , Tang Yi , Liao Yan , Liu Xinmin
Published: December 2023

Background

Uterine fibroids (UFs) are the most common benign tumors in women of reproductive age. The most effective treatment is myomectomy, but there is no long-term or low-invasive treatment option exists. Acupuncture can be used to treat UFs in a variety of ways. However, there is no meta-analytic synthesis including valid data that explored the efficacy of acupuncture for UFs.

Objective

To assess the efficacy and safety of acupuncture for treating UFs.

Methods

The PRISMA 2020 checklist was used. We identified and extracted the trials through may 2023 from six databases. The quality of the trials was assessed using the risk of bias (2.0). Meta-analysis was performed using RevMan 5.4 software, and it was synthesized using the random-effects model if the included studies were in high heterogeneity. Subgroup and sensitivity analysis were used if necessary.

Results

A total of 1,035 trials were identified, of which 11 were included in the review and meta-analysis. In terms of acupuncture scheme design and fibroid-related symptoms, the trials are highly heterogeneous. All 11 trials have reported acupuncture types, with traditional acupuncture and electroacupuncture being the more representative subgroups. A qualitative review of existing evidence shows that acupuncture has no serious adverse reaction on UFs. Meta-analysis shows that acupuncture can effectively reduce the volume of UFs (MD – 3.89, 95% CI – 5.23 to – 2.56, P < 0.00001) or uterine volume (MD – 16.22, 95% CI – 19.89 To – 12.55, p < 0.00001), reduce the score of fibroid symptoms (MD – 3.03, 95% CI − 3.45 to – 2.60, p < 0.00001), improve the treatment efficiency (RR: 0.19, 95% CI: 0.13 to 0.25, p < 0.00001), and likely do not affect the estrogen level.
Bacterial and fungal bioburden reduction on material surfaces using various sterilization techniques suitable for spacecraft decontamination
Authors: Kimura Shunta , Ishikawa Shu , Hayashi Nobuya , Fujita Kazuhisa , Inatomi Yuko , Suzuki Shino
Published: December 2023

Planetary protection is a guiding principle aiming to prevent microbial contamination of the solar system by spacecraft (forward contamination) and extraterrestrial contamination of the Earth (backward contamination). Bioburden reduction on spacecraft, including cruise and landing systems, is required to prevent microbial contamination from Earth during space exploration missions. Several sterilization methods are available; however, selecting appropriate methods is essential to eliminate a broad spectrum of microorganisms without damaging spacecraft components during manufacturing and assembly. Here, we compared the effects of different bioburden reduction techniques, including dry heat, UV light, isopropyl alcohol (IPA), hydrogen peroxide (H2O2), vaporized hydrogen peroxide (VHP), and oxygen and argon plasma on microorganisms with different resistance capacities. These microorganisms included Bacillus atrophaeus spores and Aspergillus niger spores, Deinococcus radiodurans, and Brevundimonas diminuta, all important microorganisms for considering planetary protection. Bacillus atrophaeus spores showed the highest resistance to dry heat but could be reliably sterilized (i.e., under detection limit) through extended time or increased temperature. Aspergillus niger spores and D. radiodurans were highly resistant to UV light. Seventy percent of IPA and 7.5% of H2O2 treatments effectively sterilized D. radiodurans and B. diminuta but showed no immediate bactericidal effect against B. atrophaeus spores. IPA immediately sterilized A. niger spores, but H2O2 did not. During VHP treatment under reduced pressure, viable B. atrophaeus spores and A. niger spores were quickly reduced by approximately two log orders. Oxygen plasma sterilized D. radiodurans but did not eliminate B. atrophaeus spores. In contrast, argon plasma sterilized B. atrophaeus but not D. radiodurans. Therefore, dry heat could be used for heat-resistant component bioburden reduction, and VHP or plasma for non-heat-resistant components in bulk bioburden reduction. Furthermore, IPA, H2O2, or UV could be used for additional surface bioburden reduction during assembly and testing. The systemic comparison of sterilization efficiencies under identical experimental conditions in this study provides basic criteria for determining which sterilization techniques should be selected during bioburden reduction for forward planetary protection.
Word-based GWAS harnesses the rich potential of genomic data for E. coli quinolone resistance
Authors: Malekian Negin , Sainath Srividhya , Al-Fatlawi Ali , Schroeder Michael
Published: December 2023

Quinolone resistance presents a growing global health threat. We employed word-based GWAS to explore genomic data, aiming to enhance our understanding of this phenomenon. Unlike traditional variant-based GWAS analyses, this approach simultaneously captures multiple genomic factors, including single and interacting resistance mutations and genes. Analyzing a dataset of 92 genomic E. coli samples from a wastewater treatment plant in Dresden, we identified 54 DNA unitigs significantly associated with quinolone resistance. Remarkably, our analysis not only validated known mutations in gyrA and parC genes and the results of our variant-based GWAS but also revealed new (mutated) genes such as mdfA, the AcrEF-TolC multidrug efflux system, ptrB, and hisI, implicated in antibiotic resistance. Furthermore, our study identified joint mutations in 14 genes including the known gyrA gene, providing insights into potential synergistic effects contributing to quinolone resistance. These findings showcase the exceptional capabilities of word-based GWAS in unraveling the intricate genomic foundations of quinolone resistance.
Biocrust reduces the soil erodibility of coral calcareous sand by regulating microbial community and extracellular polymeric substances on tropical coral island, South China Sea
Authors: Wang Lin , Huang Yu , Yang Qingsong , Mai Zhimao , Xie Feiyang , Lyu Lina , Zhang Si , Li Jie
Published: December 2023

Tropical coral islands assume a pivotal role in the conservation of oceanic ecosystem biodiversity. However, their distinctive environmental attributes and limited vegetation render them highly susceptible to soil erosion. The biological soil crust (biocrust), owing to its significant ecological role in soil stabilization and erosion prevention, is deemed an effective means of mitigating soil erosion on coral island. However, existing research on the mechanisms through which biocrusts resist soil erosion has predominantly concentrated on arid and semi-arid regions. Consequently, this study will specifically delve into elucidating the erosion-resistant mechanisms of biocrusts in tropical coral island environments, South China Sea. Specifically, we collected 16 samples of biocrusts and bare soil from Meiji Island. High-throughput amplicon sequencing was executed to analyze the microbial community, including bacteria, fungi, and archaea. Additionally, quantitative PCR was utilized to assess the abundance of the bacterial 16S rRNA, fungal ITS, archaeal 16S rRNA, and cyanobacterial 16S rRNA genes within these samples. Physicochemical measurements and assessments of extracellular polymeric substances (EPSs) were conducted to characterize the soil properties. The study reported a significantly decreased soil erodibility factor after biocrust formation. Compared to bare soil, soil erodibility factor decreased from 0.280 to 0.190 t h MJ−1 mm−1 in the biocrusts. Mechanistically, we measured the microbial EPS contents and revealed a negative correlation between EPS and soil erodibility factor. Consistent with increased EPS, the abundance of bacteria, fungi, archaea, and cyanobacteria were also detected significantly increased with biocrust formation. Correlation analysis detected Cyanobacteria, Chloroflexi, Deinococcota, and Crenarchaeota as potential microbials promoting EPSs and reducing soil erosion. Together, our study presents the evidence that biocrust from tropical coral island in the South China Sea promotes resistance to soil erosion, pinpointing key EPSs-producing microbials against soil erosion. The findings would provide insights for island soil restoration.
Mannitol improves Monascus pigment biosynthesis with rice bran as a substrate in Monascus purpureus
Authors: Chen Di , Li Han
Published: December 2023

To reduce the production cost of Monascus pigments (MPs), the utilization of rice bran (RB), an agricultural waste product, as a substrate in submerged fermentation was conducted in this study. To improve MP production, different nutritional ingredients including mannitol (Man), NH4NO3 (AN), ZnSO4 (Zn), and optimization (Opti), which was a synthesis of the three above ones, were added in rice bran (RB) medium. The yields of MPs, pigment constituents, and growth and development of Monascus purpureus M9 were investigated in this study. Man had the maximum color value of 3,532 U/g, which was 18.69 times more than that of RB and reached up to 76.65% of the value of rice (Rice) fermentation. Man significantly increased the production of two orange pigments, monascorubrin and rubropunctatin, of which the yields were 69.49 and 95.36% of the counterpart of Rice. The biomass and colony diameter of Opti presented the maximum value among different groups. AN and RB induced more asexual spore formation, whereas Opti and Man promoted sexual spore production. Comparative transcriptomic analysis showed that different nutritional ingredients led to changes in pigment production, promoting the growth and development of M. purpureus M9 through the regulation of related gene expression. Man and Opti improved MP production by regulating the primary metabolism, including the Embden–Meyerhof pathway (EMP), the pentose phosphate (PP) pathway, the tricarboxylic (TCA) cycle, fatty acid degradation (FAD), fatty acid biosynthesis (FAB), amino acid metabolism (AAM), and fructose and mannose metabolism (FMM), to provide the precursors (acetyl-CoA and malonyl-CoA) for MP biosynthesis. This study presents a low-cost method for increasing MP production and explains the molecular mechanisms of different nutritional ingredients for enhancing MP biosynthesis.
Case report: Right vertebral and carotid artery anomalies with an aberrant right subclavian artery in two patients
Authors: Chen Jiang , Liu LunXin , Kou Xiaobo , Wang Chaohua
Published: December 2023

Abnormal origins of the vertebral artery with supra-aortic vessel variants are exceedingly uncommon. Herein, we present two cases of the vertebral artery originating from the right common carotid artery associated with the right subclavian artery arising separately as the initial branch of the aortic arch, followed by the right common carotid artery. We reviewed the embryology of the anomalous origins of the vertebral and subclavian arteries. These variants can significantly affect surgical planning and cause severe clinical symptoms.
Biomarkers for predicting the severity of spinal cord injury by proteomic analysis
Authors: Wei Liangfeng , Huang Yubei , Chen Yehuang , Wu Jianwu , Chen Kaiqin , Zheng Zhaocong , Wang Shousen , Xue Liang
Published: December 2023

Purpose

Currently, there is a shortage of the protein biomarkers for classifying spinal cord injury (SCI) severity. We attempted to explore the candidate biomarkers for predicting SCI severity.

Methods

SCI rat models with mild, moderate, and severe injury were constructed with an electro-mechanic impactor. The behavior assessment and pathological examinations were conducted before and after SCI. Then, quantitative liquid chromatography-mass spectrometry (LC-MS/MS) was performed in spinal cord tissues with different extents of injury. The differentially expressed proteins (DEPs) in SCI relative to controls were identified, followed by Mfuzz clustering, function enrichment analysis, and protein-protein interaction (PPI) network construction. The differential changes of candidate proteins were validated by using a parallel reaction monitoring (PRM) assay.

Results

After SCI modeling, the motor function and mechanical pain sensitivity of SCI rats were impaired, dependent on the severity of the injury. A total of 154 DEPs overlapped in the mild, moderate, and severe SCI groups, among which 82 proteins were classified in clusters 1, 2, 3, 5, and 6 with similar expression patterns at different extents of injury. DEPs were closely related to inflammatory response and significantly enriched in the IL-17 signaling pathway. PPI network showed that Fgg (Fibrinogen gamma chain), Fga (Fibrinogen alpha chain), Serpinc1 (Antithrombin-III), and Fgb (Fibrinogen beta chain) in cluster 1 were significant nodes with the largest degrees. The upregulation of the significant nodes in SCI samples was validated by PRM.

Conclusion

Fgg, Fga, and Fgb may be the putative biomarkers for assessing the extent of SCI.
Effectiveness and safety of standard chemotherapy in older patients with ovarian cancer: a retrospective analysis by age group and treatment regimen
Authors: Brezis Miriam R. , Shachar Eliya , Peleg Hasson Shira , Laskov Ido , Michaan Nadav , Levy Bar , Wolf Ido , Safra Tamar
Published: December 2023

Objective

To evaluate the effectiveness and safety of standard chemotherapy administered to patients >70 years with advanced ovarian cancer (OC).

Methods

Medical records of 956 advanced-stage patients with OC treated between 2002-2020 with standard surgery and paclitaxel-carboplatin chemotherapy in a three-weekly (PC-3W) or weekly (PC-1W) regimen were reviewed. Treatment response and tolerability were compared between patients ≤70 years (N=723) and >70 years (N=233) with stratification to septuagenarians (>70-80 years) and octogenarians (>80 years).

Results

Median overall survival (mOS) in patients >70 was 41.26 months (95% confidence interval [Cl], 37.22-45.14) and median progression-free survival (mPFS) was 11.04 months (95% Cl, 8.97-15.74). No statistically significant differences in mPFS and mOS were observed between septuagenarians and octogenarians. Patients >70 treated with PC-1W versus PC-3W had significantly longer mOS (57.17 versus 30.00 months) and mPFS (19.09 versus 8.15 months). Toxicity rates were mostly similar between younger and older patients. Among patients >70 treated with PC-1W, the rate of neutropenia (75.7% versus 51.8%, p=0.0005), thrombocytopenia (41.0% versus 22.2%, p=0.0042) and anemia (78.1% versus 51.9%, p<0.0001) were significantly higher and the rate of grade 2 alopecia was statistically significantly lower compared with those >70 treated with PC-3W. Significantly more patients treated with PC-1W completed ≥6 chemotherapy cycles, suggesting better tolerability of this regimen.

Conclusions

Older patients with OC may benefit from improved OS with reasonable toxicity if treated with standard chemotherapy. Older patients treated with PC-1W are more likely to complete the full chemotherapy course and survive longer compared with those treated with conventional PC-3W.
Basis and applicability of noninvasive inverse electrocardiography: a comparison between cardiac source models
Authors: van der Waal Jeanne , Meijborg Veronique , Coronel Ruben , Dubois Rémi , Oostendorp Thom
Published: December 2023

The body surface electrocardiogram (ECG) is a direct result of electrical activity generated by the myocardium. Using the body surface ECGs to reconstruct cardiac electrical activity is called the inverse problem of electrocardiography. The method to solve the inverse problem depends on the chosen cardiac source model to describe cardiac electrical activity. In this paper, we describe the theoretical basis of two inverse methods based on the most commonly used cardiac source models: the epicardial potential model and the equivalent dipole layer model. We discuss similarities and differences in applicability, strengths and weaknesses and sketch a road towards improved inverse solutions by targeted use, sequential application or a combination of the two methods.
Epithelial-to-mesenchymal transition, inflammation, subsequent collagen production, and reduced proteinase expression cooperatively contribute to cyclosporin-A-induced gingival overgrowth development
Authors: Imagawa Mio , Shinjo Takanori , Sato Kohei , Kawakami Kentaro , Zeze Tatsuro , Nishimura Yuki , Toyoda Masaaki , Chen Shuang , Ryo Naoaki , Ahmed Al-kafee , Iwashita Misaki , Yamashita Akiko , Fukuda Takao , Sanui Terukazu , Nishimura Fusanori
Published: December 2023

Drug-induced gingival overgrowth (DIGO), induced by certain immunosuppressive drugs, antihypertensive agents, and antiepileptic drugs, may contribute to the formation of deeper periodontal pockets and intractableness in periodontitis. To date, multiple factors such as enhanced matrix production, inflammation, and reduced matrix degradation might be involved in the pathogenesis of DIGO. We have previously reported that SPOCK-1, a heparan sulfate proteoglycan, could affect gingival thickening by promoting epithelial-to-mesenchymal transition (EMT) in gingival keratinocytes. However, few studies have investigated whether a combination of these factors enhances the DIGO phenotype in animal models. Therefore, we investigated whether SPOCK-1, periodontal inflammation, and cyclosporin-A (CsA) could cooperatively promote gingival overgrowth. We first confirmed that Spock-1 overexpressing (Spock1-Tg) mice showed significantly thicker gingiva and greater alveolar bone loss than WT mice in response to ligature-induced experimental periodontitis. DIGO was induced by the combination of CsA administration and experimental periodontitis was significantly enhanced in Spock1-Tg mice compared to that in WT mice. Ligature-induced alveolar bone loss in CsA-treated Spock1-Tg mice was also significantly greater than that in CsA-treated WT mice, while being accompanied by an increase in Rankl and Col1a1 levels and a reduction in matrix metalloprotease expression. Lastly, SPOCK-1 promoted RANKL-induced osteoclast differentiation in both human peripheral blood mononuclear cells and murine macrophages, while peritoneal macrophages from Spock1-Tg mice showed less TNFα and IL-1β secretion than WT mice in response to Escherichia coli lipopolysaccharide. These results suggest that EMT, periodontal inflammation, and subsequent enhanced collagen production and reduced proteinase production contribute to CsA-induced DIGO pathogenesis.
Foliar endophyte diversity in Eastern Asian-Eastern North American disjunct tree species – influences of host identity, environment, phylogeny, and geographic isolation
Authors: Zhou Wenbin , Shi Wei , Soltis Pamela S. , Soltis Douglas E. , Xiang Qiu-Yun (Jenny)
Published: December 2023

Introduction

The well-known eastern Asian (EA) and eastern North American (ENA) floristic disjunction provides a unique system for biogeographic and evolutionary studies. Despite considerable interest in the disjunction, few studies have investigated the patterns and their underlying drivers of allopatric divergence in sister species or lineages isolated in the two areas. Endophyte diversity and assembly in disjunct sister taxa, as an ecological trait, may have played an important role in the processes of allopatric evolution, but no studies have examined endophytes in these lineages. Here we compared foliar endophytic fungi and bacteria-archaea (FEF and FEB) in 17 EA-ENA disjunct species or clade pairs from genera representing conifers and 10 orders of five major groups of angiosperms and 23 species of Cornus from EA and North America.

Methods

Metagenomic sequencing of fungal ITS and bacterial-archaeal 16S rDNA was used to capture the foliar endophytic communities. Alpha and beta diversity of fungi and bacteria were compared at multiple scales and dimensions to gain insights into the relative roles of historical geographic isolation, host identity, phylogeny, and environment from samples at different sites in shaping endophytic diversity patterns.

Results

We found that beta diversity of endophytes varied greatly among plant individuals within species and between species among genera at the same sampling site, and among three sampling sites, but little variation between region-of-origin of all plant species (EA vs ENA) and between EA-ENA disjunct counterparts within genera. Various numbers of indicator fungal species differing in abundance were identified for each plant genus and Cornus species. An overall significant correlation between endophyte community dissimilarity and phylogenetic distance of plants was detected among the disjunct genera but not among species of Cornus. However, significant correlations between beta diversities at different taxonomic scales of endophytes and phylogenetic distances of Cornus species were observed.

Discussion

Our results suggest important roles of host identity and environment (sampling sites), and a likely minor role of phylogenetic divergence and historical biogeographic isolation in shaping the pattern of foliar endophyte diversity and assembly in the EA-ENA disjunct genera and Cornus. The results lead to a hypothesis that the sister taxa in EA and ENA likely differ in FEF and FEB when growing in native habitats due to differences in local environments, which may potentially drive allopatric divergence of the functional features of species.
Sorghum bicolor INDETERMINATE1 is a conserved primary regulator of flowering
Authors: De Riseis Samuel , Chen Junping , Xin Zhanguo , Harmon Frank G.
Published: December 2023

Introduction

A fundamental developmental switch for plants is transition from vegetative to floral growth, which integrates external and internal signals. INDETERMINATE1 (Id1) family proteins are zinc finger transcription factors that activate flowering in grasses regardless of photoperiod. Mutations in maize Id1 and rice Id1 (RID1) cause very late flowering. RID1 promotes expression of the flowering activator genes Early Heading Date1 (Ehd1) and Heading date 1 (Hd1), a rice homolog of CONSTANS (CO).

Methods and results

Mapping of two recessive late flowering mutants from a pedigreed sorghum EMS mutant library identified two distinct mutations in the Sorghum bicolor Id1 (SbId1) homolog, mutant alleles named sbid1-1 and sbid1-2. The weaker sbid1-1 allele caused a 35 day delay in reaching boot stage in the field, but its effect was limited to 6 days under greenhouse conditions. The strong sbid1-2 allele delayed boot stage by more than 60 days in the field and under greenhouse conditions. When sbid1-1 and sbid1-2 were combined, the delayed flowering phenotype remained and resembled that of sbid1-2, confirming late flowering was due to loss of SbId1 function. Evaluation of major flowering time regulatory gene expression in sbid1-2 showed that SbId1 is needed for expression of floral activators, like SbCO and SbCN8, and repressors, like SbPRR37 and SbGhd7.

Discussion

These results demonstrate a conserved role for SbId1 in promotion of flowering in sorghum, where it appears to be critical to allow expression of most major flowering regulatory genes.
Increased fruit intake is associated with reduced risk of depression: evidence from cross-sectional and Mendelian randomization analyses
Authors: Yan Zhaoqi , Xu Yifeng , Li Keke , Liu Liangji
Published: December 2023

Background

The association between dietary patterns and depression has gained significant attention, but the relationship between fruit intake and depression remains unclear. This study aimed to investigate the role of fruit intake in the risk of depression using data from the National Health and Nutrition Examination Survey (NHANES) and Mendelian randomization (MR) analysis, and further explore the causal relationship between them.

Materials and methods

Cross-sectional analysis was conducted using the 2005–2018 NHANES data. Specialized weighted complex survey design analysis software was used for multivariate logistic analysis. Additionally, genetic variants for fruit intake and depression, as well as its related neuroticism traits, from the GWAS were used as instrumental variables in MR analysis. The inverse variance weighted (IVW) method was employed as the primary analysis method to evaluate the causal relationship between them. MR-Egger regression, MR-PRESSO test, and leave-one-out analysis were conducted to assess heterogeneity and pleiotropy.

Results

In NHANES, compared to the lowest quartile (Q1, <0.12 cup], the highest quartile (Q4, >1.49 cups) of fruit intake showed a significant reduction in the risk of depression after adjusting for relevant covariates. Model 3, after rigorous adjustment for multiple covariates, demonstrated improved predictive performance in both Receiver operating characteristic (ROC) curve and Decision curve analysis (DCA). In Model 3, the proportion of reduced depression risk associated with fruit intake reached 31% (OR = 0.69, 95% CI: 0.50–0.95). This association remained significant in the MR analysis (OR = 0.92, 95% CI = 0.87–0.96; p = 5.09E-04). Fruit intake was also associated with a decreased risk of neuroticism traits related to depression, including feeling lonely (OR = 0.82, 95% CI = 0.74–0.90; p = 2.91E-05), feeling miserable (OR = 0.79, 95% CI = 0.72–0.87; p = 2.35E-06), feeling fed-up (OR = 0.75, 95% CI = 0.68–0.83; p = 2.78E-08), irritable mood (OR = 0.89, 95% CI = 0.79–0.99; p = 0.03), and neuroticism (OR = 0.85, 95% CI = 0.76–0.96; p = 9.94E-03). The causal relationship between feeling lonely and fruit intake was bidirectional.

Conclusion

Increased fruit intake has a causal effect in reducing the risk of depression and is beneficial for related psychological well-being.
Biomonitoring of polycyclic aromatic hydrocarbons in firefighters at fire training facilities and in employees at respiratory protection and hose workshops
Authors: Koslitz Stephan , Heinrich Birgit , Käfferlein Heiko U. , Koch Holger M. , Pelzl Tim , Pitzke Katrin , Köster Daniel , Weiß Tobias , Harth Volker , Brüning Thomas , Behrens Thomas , Taeger Dirk
Published: December 2023

Introduction

Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic to humans and are formed by incomplete combustion. PAHs are always present during firefighting operations, and fire department members can be exposed to them in the workplace.

Methods

In this study, we analyzed 1-hydroxypyrene (1-OHP) in 36 urine samples from nine firefighters, collected before and after fire training sessions, and 32 urine samples from eight employees at respiratory protection and hose workshops. To assess breakthrough PAH exposure through personal protective equipment and potential dermal uptake, some of the workshop employees wore cotton garments under their regular workwear. Cotton samples were then examined for the presence of 17 semi-volatile and low-volatility PAHs.

Results

After firefighting exercises, we observed approximately a fivefold increase in mean 1-OHP concentrations in samples from firefighters, from 0.24 μg/L to 1.17 μg/L (maximum: 5.31 μg/L). In contrast, 1-OHP levels in workshop employees were found to be low, with the majority of urine samples yielding concentrations below the limit of quantification (LOQ: 0.05 μg/L, maximum: 0.11 μg/L). Similarly, low PAH levels were found on the workshop employees' cotton undergarments, with maximum concentrations of 250 and 205 ng/g for pyrene and benzo[a]pyrene, respectively.

Discussion

In conclusion, significant increases in 1-OHP in urine were observed in firefighters after training sessions, whereas work-related exposure remained low among workshop employees.
Standardized brain tumor imaging protocols for clinical trials: current recommendations and tips for integration
Authors: Sanvito Francesco , Kaufmann Timothy J. , Cloughesy Timothy F. , Wen Patrick Y. , Ellingson Benjamin M.
Published: December 2023

Standardized MRI acquisition protocols are crucial for reducing the measurement and interpretation variability associated with response assessment in brain tumor clinical trials. The main challenge is that standardized protocols should ensure high image quality while maximizing the number of institutions meeting the acquisition requirements. In recent years, extensive effort has been made by consensus groups to propose different “ideal” and “minimum requirements” brain tumor imaging protocols (BTIPs) for gliomas, brain metastases (BM), and primary central nervous system lymphomas (PCSNL). In clinical practice, BTIPs for clinical trials can be easily integrated with additional MRI sequences that may be desired for clinical patient management at individual sites. In this review, we summarize the general concepts behind the choice and timing of sequences included in the current recommended BTIPs, we provide a comparative overview, and discuss tips and caveats to integrate additional clinical or research sequences while preserving the recommended BTIPs. Finally, we also reflect on potential future directions for brain tumor imaging in clinical trials.
The impact of routines on emotional and behavioural difficulties in children and on parental anxiety during COVID-19
Authors: Lees Vera , Hay Rosie , Bould Helen , Kwong Alex S. F. , Major-Smith Daniel , Kounali Daphne , Pearson Rebecca M.
Published: December 2023

Background

The Covid-19 pandemic and related public health measures, including lockdowns and school closures, have impacted on mental health of children.

Aims and hypothesis

We hypothesised that there would be an association between maintaining a routine during lockdown and both lower emotional and behavioural difficulties in children and lower parental anxiety. Routine was taken as keeping to the same basic activities such as mealtimes and bedtimes. We also hypothesised that children of ‘keyworker’ parents would have fewer emotional and behavioural symptoms due to having maintained more normal routines. The key reason was that children of keyworkers still attended school or nursery and parents would have been getting up and coming home at the same times as pre-Covid. Keyworker status was defined as those whose work was essential to Covid-19 response, including work in health and social care and other key sectors.

Methods

We used data from the Avon Longitudinal Study of Parents and Children (ALSPAC) to explore associations between maintaining a routine, and emotional and behavioural difficulties in children, using linear regression models. All eligible ALSPAC-G2 participants were sent the survey and the responders are representative of the eligible G2 population. We included measures of parental anxiety. We separately explored associations with having a keyworker parent. We used the Carey Infant Temperament Questionnaire and the Revised Rutter Parent Scale for Preschool Children to establish levels of emotional and behavioural difficulties. The measures were chosen to match previous waves in multi-generations in ALSPAC where they had been shown to be predictive of later mental health in children. The scales measure emotional and behavioural problems.

Results

Two hundred eighty-nine parents completed questionnaires about their 411 children. Keeping a routine was associated with emotional and behavioural difficulty scores 5.0 points lower (95% CI −10.0 to −0.1), p = 0.045 than not keeping a routine. Parents who reported keeping a routine had anxiety scores 4.3 points lower (95% CI −7.5 to −1.1), p = 0.009 than those who did not. Children of keyworkers tended to have lower emotional and behavioural difficulty scores [−3.1 (95%CI −6.26 to 0.08), p = 0.056] than children of non-keyworkers. All models were adjusted for relevant potential confounders.

Conclusion

Maintaining a routine may be beneficial for both child emotional wellbeing and parental anxiety, although it is also possible that lower parental anxiety levels made maintaining a routine easier. Being the child of a keyworker parent during lockdown may have been protective for child emotional wellbeing.
Immunoinformatics-aided rational design of a multi-epitope vaccine targeting feline infectious peritonitis virus
Authors: Chawla Mohit , Cuspoca Andrés Felipe , Akthar Nahid , Magdaleno Jorge Samuel Leon , Rattanabunyong Siriluk , Suwattanasophon Chonticha , Jongkon Nathjanan , Choowongkomon Kiattawee , Shaikh Abdul Rajjak , Malik Tabarak , Cavallo Luigi
Published: December 2023

Feline infectious peritonitis (FIP) is a grave and frequently lethal ailment instigated by feline coronavirus (FCoV) in wild and domestic feline species. The spike (S) protein of FCoV assumes a critical function in viral ingress and infection, thereby presenting a promising avenue for the development of a vaccine. In this investigation, an immunoinformatics approach was employed to ascertain immunogenic epitopes within the S-protein of FIP and formulate an innovative vaccine candidate. By subjecting the amino acid sequence of the FIP S-protein to computational scrutiny, MHC-I binding T-cell epitopes were predicted, which were subsequently evaluated for their antigenicity, toxicity, and allergenicity through in silico tools. Our analyses yielded the identification of 11 potential epitopes capable of provoking a robust immune response against FIPV. Additionally, molecular docking analysis demonstrated the ability of these epitopes to bind with feline MHC class I molecules. Through the utilization of suitable linkers, these epitopes, along with adjuvants, were integrated to design a multi-epitope vaccine candidate. Furthermore, the stability of the interaction between the vaccine candidate and feline Toll-like receptor 4 (TLR4) was established via molecular docking and molecular dynamics simulation analyses. This suggests good prospects for future experimental validation to ascertain the efficacy of our vaccine candidate in inducing a protective immune response against FIP.
Youth–Therapist and Parent–Therapist Match and Mismatch on Internalizing and Externalizing Treatment Goals as Predictors of Treatment Engagement
Authors: Yeh May , Diaz Devynne , Zerr Argero , Macias Alisandra , McCabe Kristen
Published: December 2023

Therapist–client cognitive match upon key constructs such as treatment goals is purported to be an important component of culturally competent care. For adolescent clients, treatment may involve both youths and their parents, suggesting the need to consider both youth–therapist and parent–therapist perspectives. This longitudinal study examined broadband youth–therapist and parent–therapist treatment goal matching and mismatching in relationship to treatment engagement in a culturally diverse sample of 245 outpatient mental health service-using youth. Although goal matching/mismatching did not uniformly predict treatment engagement as measured by a total score, youth–therapist internalizing goal matching predicted better youth engagement, and parent–therapist externalizing goal mismatch marginally predicted worse parent engagement. When selected post hoc analyses examined relationships to four individual engagement dimensions, youth–therapist internalizing goal matches positively predicted youth Client–therapist interaction, Communication/Openness, Client’s perceived usefulness of therapy, and Collaboration with treatment, while parent–therapist externalizing goal mismatch negatively predicted parent Collaboration with treatment. Findings support the importance of cognitive match on treatment goals as well as the consideration of both parent and youth perspectives, matched and mismatched goals, internalizing and externalizing goals, and examining individual dimensions in addition to total scores of engagement.
Biological Characterisation of Hailstones from Two Storms in South Brazil
Authors: Mantoani Maurício C. , Quintino Thaysla Beluco , Emygdio Ana Paula M. , Guerra Lara C. C. , Dias Maria A. F. S. , Dias Pedro L. S. , Rodrigues Fábio , Silva Dulcilena M. C. , Duo Filho Valter Batista , Rudke Anderson Paulo , Alves Ronaldo Adriano , Martins Leila Droprinchinski , Martins Jorge Alberto , Siqueira Alexandre , Boschilia Solana M. , Carotenuto Federico , Šantl-Temkiv Tina , Phillips Vaughan , Gonçalves Fábio L. T.
Published: December 2023

Although studies focusing on the physicochemical properties of aerosols/clouds have not been performed extensively, even less attention has been given to hailstones and their biological composition. Here, we present the results of the physical and microbiological characterisation of 20 hailstones collected in Southern Brazil originating from two storms. Nearly half of the hailstones (9 out of 20, or 45%) did not contain any cultivable bacteria or fungi. A total of 18 bacterial species were found in hailstones from both storms, and the genus Bacillus was found in 5 out of the 11 hailstones, with Bacillus cereus being the most frequent bacterial species. Fungi, on the other hand, were only present in four hailstones derived from a single storm, with three fungal species identified and Epicoccum nigrum being the most frequent fungal species. HYSPLIT modelling indicated the different flow of air masses from the Amazon and Pacific Ocean that contributed to the loading of microorganisms found in the clouds at the time of the two storms. Our findings suggest that ca. 50% of hailstones have cultivable bacterial or fungal species, which came mainly from the local landscape with intrusions of air masses derived from the Amazon and the Pacific Ocean.
Optical Design of a Miniaturised Solar Magnetograph for Space Applications
Authors: Calcines Rosario Ariadna , Green Lucie M. , Smith Alan , Long David M.
Published: December 2023

Measuring the Sun’s magnetic field is a key component of monitoring solar activity and forecasting space weather. The main goal of the research presented in this paper is to investigate the possibility of reducing the dimensions and weight of a solar magnetograph while preserving its optical quality. This article presents a range of different designs, along with their advantages and disadvantages, and an analysis of the optical performance of each. All proposed designs are based on the magneto-optical filter (MOF) technique. As a result of the design study, a miniaturised solar magnetograph is proposed with an ultra-compact layout. The dimensions are 345 mm × 54 mm × 54 mm, and the optical quality is almost at the diffraction limit. The design has an entrance focal ratio of F/17.65, with a plate scale of 83.58 arcsec/mm at the telescope image focal plane, and produces a magnification of 0.79. The field of view is 1920 arcsec in diameter, equivalent to ±0.27 degrees, sufficient to cover the entire solar disk.
Drivers for the Adoption of Organic Farming: Evidence from an Analysis of Chinese Farmers
Authors: Xia Maosen , Xiang Pingan , Mei Guo , Liu Zhizhen
Published: December 2023

Adoption decision is an important topic in organic farming research. In order to understand farmers’ decision-making, it is necessary to delve into the factors influencing their behavior. Some studies have used social psychology models to explore the adoption intention of farmers in specific locations regarding organic farming, but there is a lack of investigation into the differences in driving factors for adoption intention among farmers in the pre-organic conversion (conventional), mid-conversion (conversion), and post-conversion (certified) stages, as well as the examination of the relationship between intention and behavior. This study aims to address this issue by examining the driving factors of Chinese farmers’ adoption of organic farming practices. We established a theoretical framework based on the Theory of Planned Behavior (TPB) and applied Partial Least Squares–Structural Equation Modeling (PLS-SEM) to analyze intention data collected from 432 farmers and behavior data collected one year later. The study found that attitude, perceived behavioral control, subjective norms, and descriptive norms positively drive the intention to adopt organic farming. In addition to intention being a determinant of behavior, farm size also positively influences behavior. The strength of the impacts of subjective norms on intention and farm size on behavior differs between conventional farmers and conversion farmers. The common driving chain of “attitude → intention → behavior” exists in the organic adoption decision of conventional, conversion, and certified farmers. Our findings suggest that the public sector can attract conventional farmers to transition to organic and stabilize existing practitioners of organic agriculture practices by considering the differences in driving factors when formulating intervention policies.
Effects of Cellulosic Carbon Addition on Nitrogen Removal from Simulated Dry Land Drainage, and Its Environmental Effects
Authors: Duan Jingjing , Cao Pengpeng , Shu Tong , Zhou Beibei , Xue Lihong , Yang Linzhang
Published: December 2023

Agricultural non-point source pollution has emerged as a significant driver of declining global water quality in recent years. Ditch systems hold considerable promise for trapping and purifying pollutants. However, the persistent challenge has been the limited availability of carbon sources in drainage water, which significantly hinders nitrogen (N) removal in ditches. This study investigated the dynamic changes in ammonia (NH4+) and nitrate (NO3−) levels caused by three cellulosic carbon additions (rice straw, coir, and sawdust) during both winter and summer seasons. Water column devices were used as containers, and the impacts on environmental factors and water denitrification rates were explored. Results demonstrated that the addition of straw exhibited the most effective N removal in winter and summer, and significantly enhanced water denitrification rates in a short timeframe, with the maximum denitrification rate reaching 1482.42 μmol·L−1·h−1. However, there was an observed accumulation of NH4-N and chemical oxygen demand (COD) in summer. Also, the addition of sawdust resulted in a notable increase in greenhouse gas emissions during the summer test. In conclusion, during the cooler seasons of winter and spring when temperatures are not as high, the combined use of various cellulosic carbon sources has the potential to enhance water denitrification and mitigate adverse environmental impacts, offering valuable applications for water quality improvement.