bluuebunny/crossref_metadata_2025 huggingface dataset:
A phase 1, open-label, multicenter study to assess the safety, tolerability, and immunogenicity of mRNA-4157 alone in subjects with resected solid tumors and in combination with pembrolizumab in subjects with unresectable solid tumors (Keynote-603).
Authors: Burris III Howard A. , Patel Manish R. , Cho Daniel C. , Clarke Jeffrey Melson , Gutierrez Martin , Zaks Tal Z. , Frederick Joshua , Hopson Kristen , Mody Kinjal , Binanti-Berube Alverina , Robert-Tissot Celine , Cowens Kristen , Breton Ben , Zhong Shan , Zhou Honghong , Cohen Pamela Sarah , Keating Karen , Meehan Robert S. , Gainor Justin F.
Published: October 2019

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Background: T-cell targeting of mutation-derived epitopes (neoantigens) has been demonstrated to drive anti-tumor responses. Immunizing patients against such neoantigens in combination with a checkpoint inhibitor (CPI) may elicit greater anti-tumor responses than CPI alone. Mutations are rarely shared between patients, thus requiring a personalized approach to vaccine design. Methods: This is an interim report of a phase I dose escalation study of mRNA-4157 given as monotherapy in patients with resected solid tumors and in combination with pembrolizumab in patients with unresectable solid tumors. mRNA-4157 is a lipid encapsulated personalized vaccine encoding multiple neoantigens selected using a proprietary algorithm designed to induce neoantigen specific T cells and associated anti-tumor responses. Patients received up to 9 cycles (Q3W) of mRNA-4157 by IM injection (0.04 – 1 mg). In combination arm, pembrolizumab (200 mg) was administered for two cycles prior to combination with mRNA-4157 for up to 9 cycles and may continue on pembrolizumab monotherapy for up to 2 years. Results: As of 10-May-2019, 33 patients received mRNA-4157 alone or in combination. No DLTs or related SAEs or AEs ≥ grade 3 were reported. Of the 13 patients treated with monotherapy (3 melanoma, 8 NSCLC, 2 MSI-high CRC), 11 patients remain disease free on study, median follow-up of 10 months. Of the 20 patients treated in combination (1 TMB-high metastatic cutaneous squamous cell, 4 bladder, 2 HNSCC, 1 melanoma, 7 NSCLC, 2 SCLC, 3 MSI-high (CRC, prostate, endometrial), 13 had received prior CPI, 5 PRs (2 in patients previously treated with PD-1/L1 inhibitors), 6 SD, and 8 PD were reported. Neoantigen specific CD8+ T-cell responses have been detected. Conclusions: mRNA-4157 is safe and well tolerated at all dose levels tested. Clinical responses have been observed in combination with pembrolizumab and neoantigen-specific T cells have been induced, supporting the advancement of mRNA-4157 to phase 2. Clinical trial information: NCT 03739931.
Treatment-Induced Neuropathy of Diabetes (TIND) in Pediatrics: A Case Report and Review of the Literature
Authors: Chandler Erika , Brown Martin , Wintergerst Kupper , Doll Elizabeth
Published: October 2019

Context

Treatment-induced neuropathy of diabetes (TIND) is a rarely reported but important consideration in patients presenting with an acute onset of neuropathic symptoms following rapid correction of hyperglycemia in diabetes. Although it has been reported in children, the preponderance of literature focuses on adults with TIND.

Case Description

We report an 18-year-old male with this condition and his clinical course. We then discuss the proposed pathophysiology of TIND and review the literature. We also provide a standard workup for the diagnosis of TIND.

Conclusion

In both pediatric and adult populations, TIND should be considered in diabetic patients who develop neuropathy acutely following rapid correction of hyperglycemia. Because the pathophysiology of TIND remains poorly understood, there is insufficient information regarding how to target susceptible individuals and prevent the development of TIND.
Growth and toxicity of Halomicronema metazoicum (Cyanoprokaryota, Cyanophyta) at different conditions of light, salinity and temperature
Authors: Mutalipassi Mirko , Mazzella Valerio , Romano Giovanna , Ruocco Nadia , Costantini Maria , Glaviano Francesca , Zupo Valerio
Published: October 2019

Cyanobacteria may live in the water column and in the benthos of aquatic environments, or be symbionts of other organisms, as in the case of Phormidium-like cyanobacteria, known to influence the ecology of freshwater and marine ecosystems. A strain of Phormidium-like cyanobacteria has been recently isolated as a free-living epiphyte of leaves of Posidonia oceanica (L.) Delile in the Mediterranean sea and its biology and ecology is herein investigated. It was identified as Halomicronema metazoicum, previously known uniquely as a symbiont of marine sponges. We cultivated it in a range of light irradiances, temperatures and salinities, to establish the most suitable conditions for the production of allelopatic and toxic compounds. The bioactivity of its spent culture medium was measured by means of standard toxicity tests performed on two model organisms. Our results indicate that at least two bioactive compounds are produced, at low and high irradiance levels and at two temperatures. The main compounds influencing the survival of model organisms are produced at the highest temperature and high or intermediate irradiance levels. The present research contributes to the understanding of critical toxigenic relationships among cyanobacteria and invertebrates, possibly influencing the ecology of such a complex environment as P. oceanica. Future isolation, identification and production of bioactive compounds will permit their exploitation for biotechnologies in the field of ecological conservation and medical applications.
Data Analytics, Innovation, and Firm Productivity
Authors: Wu Lynn , Hitt Lorin , Lou Bowen
Published: October 2019

We examine the relationship between data analytics capabilities and innovation using detailed firm-level data. To measure innovation, we first utilize a survey to capture two types of firm practices, process improvement and new technology development for 331 firms. We then use patent data to further analyze new technology development for a broader sample of more than 2,000 publicly traded firms. We find that data analytics capabilities are more likely to be present and are more valuable in firms that are oriented around process improvement and that create new technologies by combining a diverse set of existing technologies than they are in firms that are focused on generating entirely new technologies. These results are consistent with the theory that data analytics are complementary to certain types of innovation because they enable firms to expand the search space of existing knowledge to combine into new technologies, as well as the theoretical arguments that data analytics support incremental process improvements. Data analytics appears less effective for developing entirely new technologies or creating combinations involving a few areas of knowledge, innovative approaches where there is either limited data or limited value in integrating diverse knowledge. Overall, our results suggest that firms that have historically focused on specific types of innovation—process innovation and innovation by diverse recombination—may receive the most benefits from using data analytics.

This paper was accepted by Chris Forman, information systems.
Advance Service Reservations with Heterogeneous Customers
Authors: Stein Clifford , Truong Van-Anh , Wang Xinshang
Published: October 2019

We study a fundamental model of resource allocation in which a finite number of resources must be assigned in an online manner to a heterogeneous stream of customers. The customers arrive randomly over time according to known stochastic processes. Each customer requires a specific amount of capacity and has a specific preference for each of the resources with some resources being feasible for the customer and some not. The system must find a feasible assignment of each customer to a resource or must reject the customer. The aim is to maximize the total expected capacity utilization of the resources over the horizon. This model has application in services, freight transportation, and online advertising. We present online algorithms with bounded competitive ratios relative to an optimal off-line algorithm that knows all stochastic information. Our algorithms perform extremely well compared with common heuristics as demonstrated on a real data set from a large hospital system in New York City.

This paper was accepted by Yinyu Ye, optimization.
The Tutoring Influences in Distance Education at El Oro Province Ecuador
Authors: Espinoza-Freire Eudaldo Enrique , Rojas-Garcia Carlos R.
Published: October 2019

El Oro province undergoes a high rate of student’s withdrawal from distance courses offered by the local university. The distance education model gives for granted that the regular teachers are proficient for remote tutoring. This research attempts to reveal the state-of-art of this educative model in a provincial university. The study focused on a quantitative-qualitative explorative approach to determine, whether, if withdrawal is more frequent in introductory courses or not. Among the main causes for withdrawal were found: (1) the naive of learners in high education programs, (2) the inconsistency of the university proposals (academic and administrative) and; (3) the most important, the deficiencies in remote tutoring.
The Use of PSPP Software in Learning Statistics
Authors: Sto.-Tomas Minerva , Tindowen Darin Jan , Mendezabal Marie Jean , Quilang Pyrene , Agustin Erovita Teresita
Published: October 2019

This descriptive and correlational study investigated the effects of using PSPP in learning Statistics on students’ attitudes and performance. The respondents of the study were 200 Grade 11 Senior High School students who were enrolled in Probability and Statistics subject during the Second Semester of School Year 2018-2019. The respondents were randomly selected from those classes across the different academic strands that used PSPP in their Probability and Statistics subject through stratified random sampling. The results revealed that the students have favorable attitudes towards learning Statistics with the use of the PSPP software.  The students became more interested and engaged in their learning of statistics which resulted to an improved academic performance.
University of Education: Structure and Main Components of Management
Authors: Fedorov Aleksandr A. , Sedykh Ekaterina P. , Mialkina Elena V.
Published: October 2019

The article analyzes the evolution of pedagogical systems and the practice of universities in the world. The work reveals the essence and main structural components of the university education, as a special institution of higher education, designed to become a systemic solution to the problem of management and development of teacher education. The model presented by us includes the following components: education, as a platform for transferring innovative solutions into practice; Mega-Science -a system of research collaborations that provides advanced solutions to global problems; technologies -research constructs according to the Lego principle, generating local problem-solving based on a cluster; implementation -a system of investment decisions on the implementation of developments in the practice of activity; innovation is a system for the formation of products open to use that stimulate the development of education. The paper presents the main results of these developments, conclusions are made about the need to form a university of education as a special system operating within the framework of the concept of open pedagogical education and aimed at creating a federal scientific and educational hub of global importance for reproduction and the formation of human capital in scientific, engineering activities, the generation of new solutions in the field of training and development of teaching staff in.
Reading Difficulty and its Remediation: A Case Study
Authors: Akyol Hayati , Boyaci-Altinay Yasemin
Published: October 2019

The aim of this study, in which one of the qualitative research approaches, the case study design, was used, was to remedy reading problems and develop reading skills in a fourth grade primary school student with sound, syllable and word recognition exercises. The study covers 38 lesson periods in the autumn term of the 2018-2019 academic year. For developing reading skills, the strategies of “prior listening to the paragraph”, “repetitive reading” and “word repetition” were used. For determining reading errors, the “Error Analysis Inventory” was used. Data were obtained by means of observation and document examination. In the study related to determining reading status, it was established that the student had made errors such as repetition, syllabication, omission, addition and failure to notice punctuation marks. Based on the data obtained, first of all, sound, syllable and word recognition exercises were conducted with the student, and then reading exercises were carried out with texts selected to suit the level of the student’s Turkish course books and story books. Analyses were performed by taking audio and video recordings of all exercises. As a result of the intervention, the student’s desire to read increased, he began to read out loud, he began to identify sounds that he previously did not recognise or confused, and improvements in his reading skill were observed.
Factors Affected the Psychological Trauma of Children Living in Incomplete Families – The Concern in Vietnamese School Counseling
Authors: Duc Hoi Dinh , Van Son Huynh , Diem My Nguyen Thi , Thien Vu Giang
Published: October 2019

Domestic violence, separation and divorce are becoming increasingly common and leaving psychological traumas that are not easily healed for family members, especially for children in Vietnamese incomplete family. In this article, we identify the factors affecting the psychological trauma of children in the incomplete families by using Achenbach T. M’s Children Behavior Checklist (CBCL). We design 2 experiments and 3 steps to filter the main factor affected the psychological trauma of children living in incomplete families. In results, we found out that up to 46.42% of children had psychological traumas when living in incomplete family with an extremely high level of 8.16% and an average of 57.14%. The results showed not only by living in incomplete family but also other factors such as the pressure from public opinion and community affected the children psychological trauma. It is necessary to minimize the effect of psychological trauma of children in Vietnamese families.
According to Whom, Who is Who?: Perceptions Regarding Cultural, Educational Diversity and Othering (Amsterdam, the Netherlands Example)
Authors: Yaman-Ortas Banu
Published: October 2019

This study has been carried out with the families who migrated from Turkey and settled in Amsterdam, the Netherlands, which has an intense cultural and educational diversity. Finding the answer to the question “According to whom; who is who?” constitutes the fundamental ground of this research. However, this research has also been carried out in order to comprehend the points of view that have changed or have not changed between the past and the present and to scrutinize the reasons for such points of view. Moreover, another main objective is to form a comparative opinion about the situations that the settled migrant families in Amsterdam, the Netherlands, and their children have encountered regarding the “other” concept, based on cases in their experiences, with regard to the adaptation concept. Content analysis, which is a qualitative research technique, was used during the research, whereas relevant persons have been contacted by means of the snowball sampling technique. In accordance with the obtained opinions and with the results derived from sample experiences, there surely exist differences in experiences in terms of adaptation and the marginalization-othering concept between those who migrated in the past and those migrating in the present day. Those sample experiences are discussed directly and the comments have been made by considering negative and positive concepts together.
The Effects of Anxiety, Shyness and Language Learning Strategies on Speaking Skills and Academic Achievement
Authors: Oflaz Adnan
Published: October 2019

The purpose of this research was to investigate the relationship between foreign language anxiety, shyness, language learning strategies, speaking scores and academic achievement of university preparatory students learning German. In addition, it was aimed to determine how the independent variables predict the speaking scores and academic achievement. The research sample consisted of 110 students (75 female and 35 males). Three instruments used in the study were: Foreign Language Classroom Anxiety Scale; Strategy Inventory of Language Learning and Shyness Scale. The results of this study revealed that a moderate significant negative relationship (r= -.434) was present between the students' foreign language learning anxiety and academic achievement. Besides, a significant and negative relationship (r= -.290) was found between foreign language anxiety and speaking scores of students. According to the correlation analysis, no significant relationship was identified between shyness, academic achievement and speaking scores of students. According to the analysis, a significant positive relationship was found between the students' language learning strategies and their academic achievements (r= .275). Namely, these data showed that, the level of academic achievement increases as the use of strategy increases. Similarly, shyness and foreign language anxiety show a moderate positive correlation (r= .419). According to these findings, it may be stated that shyness increases as speaking anxiety rises. Besides, it was determined that students' shyness, foreign language anxiety and language learning strategies predicted 26.4 % of their academic achievement. The results indicated that independent variables were positive predictors of students’ academic achievement. Finally, suggestions were made for German teachers to reduce the effects of shyness and anxiety in the process of foreign language learning.
Voluntary ‘organic’ leadership for community resilience
Authors: Lough Benjamin J.
Published: October 2019

This article investigates the qualities of bottom-up leadership that emerges voluntarily and collectively in response to adverse events. With an eye on better understanding the resilience of marginalised communities in the Global South, it seeks to illustrate how bottom-up ‘organic’ leadership is a clear manifestation of place leadership at the local level. Findings are drawn from qualitative field data gathered in 10 Southern communities. These data illustrate that people are often willing and able to organise organically in response to adversity – and are largely successful at navigating the complex challenges they encounter. However, the long-term sustainability of organic leadership in self-organised groups often requires balanced supports from external actors. Better recognition of the added value of voluntary self-organisation happening in vulnerable communities can provide a platform for more innovative, experimental and co-creative solutions to manage risk.
III All-Russian Congress of Endocrinologists
Authors: Balabolkin M. I.
Published: October 2019

The III All-Russian Congress of Endocrinologists was held in Moscow from June 4 to 7, 1996. The scientific program of the congress included almost all sections of endocrinology: diabetes mellitus, diseases of the hypothalamic-pituitary system, diseases of the thyroid and parathyroid glands, pathology of the adrenal glands and gonads. A separate symposium at the congress was devoted to endocrine pathology as a consequence of radiation disasters. The first plenary lecture at the congress was dedicated to the late complications of diabetes, which was presented by the president of the Russian Acad Endocrinologists Association. RAMS I.I. Dedov. The report noted that one of the most pressing issues in diabetology is the prevention and treatment of late complications of diabetes, which lead to early disability, while remaining the main cause of mortality in this disease. Modern diagnostics of diabetes complications and complex therapy can already change the fate of patients with diabetes. In particular, the report provided compelling data on the early diagnosis of nephropathy at the stage of hyperfiltration and microalbuminuria.Optimal compensation of carbohydrate metabolism and the use of antihypertensive drugs, and primarily angiotensin-converting enzyme inhibitors (renitec, ramipril, enalapril, capoten, etc.) allow the development of nephropathy to be slowed down for a considerable time.The treatment tactics for diabetic retinopathy have changed. Laser coagulation is deservedly highlighted; it leads to a halt in the progression of retinopathy and preservation of vision for many years.
Recovery of degraded area of Urochloa decumbens with turkey litter fertilizer
Authors: Silva Andrisley  Joaquim da , Cunha Fernando França da , Roque Cassiano Garcia , Ribeiro Diego Oliveira , Carballal Manuel Rodriguez , Silva Monice Donatila Tavares da
Published: October 2019

The aimed of this study was to evaluate the effects of different dosages of turkey litter on soil’s chemical attributes and yield of the Urochloa decumbens in classified as degraded pasture. The work was performed at the municipality of Mineiros-GO between October/2011 and September/2013 in Quartzipsamment soil. The treatments consisted in applications of 0, 3, 6, 9 and 12 Mg ha-1 of turkey litter at a randomized-complete blocks design with four replications. Was evaluated the fodder’s productivity and the following soil’s chemical attributes: organic matter, hydrogenionic potential (CaCl2), phosphorus (resin), potassium, calcium, magnesium, cation exchange capacity and base saturation of soil. In general terms, the increase of turkey litter’s doses increased the grass productivity and the supply of nutrients of the soil, maintaining the balance of the soil-plant system. The dosage of 12 Mg ha-1 year-1 of turkey litter ensure higher amounts in the soil’s chemical attributes and in the productivity of dry mass of Urochloa decumbens.
Decomposition of cover crop mulch and weed control under a no-till system for organic maize
Authors: Brito Lamara Freitas , Galvão João Carlos Cardoso , Giehl Jeferson , Coellho Steliane Pereira , Campos Silvane de Almeida , Barrella Tatiana Pires , Dos Santos Tamara Rocha , Mendonça Beatriz Ferreira , De Jesus Edio Vicente
Published: October 2019

The decomposition dynamics of cover crop mulch influence the nutrient supply of successor crops and weed suppression. This is even more relevant in organic production systems, due to their limited use of chemical fertilizers and herbicides. As such, the aim of this study was to quantify biomass production, model the decomposition and N, P and K release of the mulch of different cover crops, and assess the weed suppression of cover crops in the form of mulch and in consortium with organic maize. A randomized block design was used, with a 7x2 factorial scheme (7 cover crop management strategies and 2 cropping systems - maize in monoculture and intercropped with jack bean) and 4 replicates. The management practices that produced the most biomass were white lupine intercropped with black oat and the white lupine, black oat and sunflower monocultures. The use of cover crops did not differ from manual weeding in terms of weed biomass, but did affect the relative importance (RI) of nutgrass. Additionally, maize intercropped with jack bean reduced weed biomass in subsequent crop growth stages.
Mobility and distribution of whey solutions in soil columns
Authors: Silva Nara Cristina de Lima , Ruiz Hugo Alberto , Almeida Leandro da Silva
Published: October 2019

In Brazil’s food industry, dairy production is one of the most important sectors, whose most relevant byproduct is whey. Due to the difficulties of reuse and environmental impacts caused when discarded as effluent in water bodies, an alternative for its final destination would be the application of this residue in the soil. The purpose of this study was to determine chemical changes and mobility and distribution of solutes in the soil after applications of whey rates, as well as to analyze the leachate collected after each application. The test was carried out in a laboratory, in PVC columns filled with soil. The treatments consisted of 2 x 2 samples of a typical dystrophic Red-Yellow Oxisol   (Oxisol) and a typical dystrophic fluvic Inceptisol (Inceptisol), sampled in the layers 0- 20 and 20-40 cm. Each experimental unit consisted of 11 PVC rings (diameter of 6.6 cm, height of 7 cm). The columns were arranged in a randomized complete block design with five replications. Four whey rates were applied, corresponding to a soil pore volume of 0.2, at intervals of six days. The leachate was collected 24 and 120 hours after each application to measure pH, electrical conductivity (EC), chemical oxygen demand (COD), contents of total N, N-NH4+, N-NO3-, Na, K, Ca, and Mg. Six days after the last whey application, the columns were opened and the soil of each ring was analyzed for pH, EC, total N, N-NH4+, N-NO3-, Na, K, Ca, and Mg. The high electrolyte concentrations of whey resulted in a general increase in soil EC. The increase of N-NH4+ and N-NO3- in the soil was high due to mineralization. High concentrations of K, Na and Ca caused displacement of Mg from the exchange complex. It was concluded that from an environmental standpoint, whey soil application is a viable alternative, given that problems of salinization and leaching of undesirable elements are avoided by an adequate management.
Wastewater from swine farming in the growth and nutrition of Khaya senegalensis (DESR.) A Juss seedlings
Authors: Araújo Emanuel França , Arauco Adriana Miranda de Santana , Dias Bruna Anair Souto , Lacerda Julian Júnio de Jesus , Boechat Cácio Luiz , Porto Dayara Lins , Arauco Luis Ricardo Romero
Published: October 2019

This study evaluated the use of wastewater from swine farming in the growth and nutritional balance of Khaya senegalensis (Desr.) A. Juss. (African mahogany) seedlings. The experiment was setup in a shade house on the Professor Cinobelina Elvas Campus of the Federal University of Piauí, in Bom Jesus, in the State of Piauí, Brazil. The experimental design was completely randomised, with five concentrations of swine farm wastewater (SFW) (0, 25, 50, 75 and 100%) added to the irrigation water. The growth and nutritional balance of the seedlings were evaluated 100 days after sowing, by measuring shoot height (H), stem diameter (SD), number of leaves (NL), total chlorophyll (TC), leaf area (LA), shoot dry weight (SDW) and root dry weight (RDW), and by calculating the total dry weight (TDW), leaf (LBA), stem (SBA), and root (RBA) biomass allocation, Dickson Quality Index (DQI) and average Nutritional Balance Index (NBIm). It was found that K. senegalensis seedlings responded to the SFW, showing the best results for growth and nutritional balance at concentrations of around 50%.
Nitrogen doses in topdressing affect vegetation indices and corn yield
Authors: Baio Fabio Henrique Rojo , Silva Eder Eujácio da , Scarpim Igor Mendes , Campos Cid Naudi da Silva , Teodoro Paulo Eduardo
Published: October 2019

Nitrogen is the main nutrient required by corn crop, especially in Cerrado soils. Remote sensing techniques can be used to generate additional information now of nitrogen fertilization recommendation. This work investigated the association of plant height and dry matter phenological variables together with NDVI, REDEDGE, SAVI, and IV 760/550 vegetation indices (VIs) with corn grain yield, under different N doses. Sowing occurred in November 2016, at a spacing of 0.45 m between rows and a 60,000 ha-1 plant population. Four N doses (0, 80, 160, and 240 kg of N ha-1) were applied at phenological stage V4. The experimental design consisted of randomized blocks, containing four N doses in topdressing and 16 replications. The active optical sensor Crop Circle ACS-470 was used to obtain the VIs. The NDVI, SAVI, and RE indices have a high positive association with each other and with the variables plant height and dry matter. Polynomial regression equations were adjusted between the variables in response as doses of N. Afterwards, they were estimated as correlations between variables and results expressed through the network of correlations. Finally, a multivariate analysis of canonical variables was performed to understand the interrelationship between the variables and each dose of N applied. NDVI and RE have a positive relationship of moderate magnitude with grain yield in corn crops.
Vigor tests in seeds creole of Phaseolus lunatus L.
Authors: Nascimento Maria das Graças Rodrigues do , Silva Maria Lúcia Maurício da , Alves Edna Ursulino , Rodrigues Caroline Marques , Silva Maria Joelma da
Published: October 2019

Seeds with a good physiological quality are essential to high productivity. However, for some seeds, Phaseolus lunatus L., the viability and vigor analyze show deficiency in quality. This work aimed to evaluate the physiological quality of Phaseolus lunatus L. seeds using several vigor tests. The study was realized at the Laboratory of Seed Analysis of the Agricultural Sciences Center of the Federal University of Paraíba, in Areia – PB, Brazil. We used seeds from five cultivars of P. lunatus (Branca, Orelha de Vó, Cearense, Rosinha, and Roxinha). To determine the physiological quality, we measured the water content, the viability and vigor test (germination, emergence, electrical conductivity, tetrazolium, first germination count and emergence, seedling length and dry mass). We used a completely randomized design for seeds evaluation, and the field test was performed in randomized blocks. The water content of P. lunatus did not differ among the cultivars, indicating a uniformity in the percentage of moisture. Also, the percentage and speed of emergency and the dry mass of seedlings did not differ among the varieties. The tetrazolium and electrical conductivity tests indicated that Rosinha cultivar had the worst viability concerning the other cultivars, showing a low percentage of living tissue and lower electrical conductivity. The seeds of Roxinha cultivar had the better physiological quality, and the Cearense cultivar had the worst quality evaluation. We recommend the tetrazolium test but not the electrical conductivity test to evaluate the vigor of P. lunatus.
Acute toxicity of Jatropha curcas oil on plant cell cycle
Authors: Andrade-Vieira Larissa Fonseca , Botelho Carolina Mastella , Laviola Bruno Galvêas , Ferreira Adésio , Fontes Milene Miranda Praça
Published: October 2019

Today, a great interest in Jatropha-based products exists worldwide, mainly for the production of biofuel. However, the oil obtained from this plant is known to be toxic due to contained curcins and phorbol esters. Bioassays, including plant cytogenetic assays based on cell cycle observation, are useful for determining the toxicity of J. curcas oil. Hence, the aim of this study was to describe the mechanism of action of J. curcas oil by cell cycle analysis using Lactuca sativa as plant testing model. A decrease in root growth was observed, closely related to the reduction in mitotic index, along with an increase in condensed nuclei. J. curcas chemicals act both as aneugenic agents, leading to the formation of lagged, sticky chromosomes and c-metaphase cells, as well as clastogenic agents, inducing the formation of chromosome bridges and fragments. The cytotoxicity and genotoxicity of phorbol esters and other chemical components of J. curcas oil was determined and discussed.
Ovarian dysgerminoma causing multifocal metastases in young bitch
Authors: Gonçalves Dias Fernanda Gosuen , Magalhães Larissa Fernandes , Magalhães Geórgia Modé , Castro Sasahara Tais Harumi de , Pereira Lucas de Freitas , Calazans Sabryna Gouveia , Gonçalves Dias Luis Gustavo Gosuen , Rezende Luara Rodrigues
Published: October 2019

The ovarian neoplasias are unusual in canine species, mainly originated from germinative cells. Among these, dysgerminoma is a rare neoplasm in bitches, predominantly affecting senile and small and medium breeds. They are large, rounded, smooth surface, painless and firm in consistency. The clinical signs commonly demonstrated by patients with this neoplasia are abdominal distension and secondary signs of hyperestrogenism. The diagnosis should be based on the association of history, clinical signs, radiographic, ultrasonographic, cytological, histopathological and immunohistochemical exams. The treatment is the surgical castration and metastases are uncommon. Due to the rarity in bitches, specifically the young animals, this study aimed to describe a case of left ovarian dysgerminoma, of considerable size and adherence in adjacent organs, in a Brazilian Fila, whose showed considerable increase in abdominal volume and progressive weight loss. The diagnosis was confirmed by means of complementary x-ray, ultrasound, histopathological and immunohistochemical tests. Even with the recommended surgical treatment (castration), the patient demonstrated severe respiratory symptoms and increased abdominal volume after two months, and the complementary exams suggested pulmonary and multifocal metastases and the tutor chose euthanasia. As presented by the results, although rare in bitches, dysgerminoma should be included in the differential of ovarian neoplasias, including the young ones.
UM ESTUDO DOS GÊNEROS ORAIS AULA ESPETÁCULO E AULA SHOW
Authors: Pereira Daniervelin Renata Marques
Published: October 2019

Este artigo dedica-se à caracterização da Aula Espetáculo (AE) e da Aula Show (AS), bem como ao estabelecimento da diferença entre elas, discutindo se AE e AS são ou não realmente novos gêneros. São consideradas ainda as semelhanças e diferenças com outros gêneros, como o espetáculo, a aula, a conferência e a oficina. Para isso, utilizamos as categorias de gênero a partir de Bakhtin. Observou-se que a Aula Espetáculo é um gênero que mescla características de aula e espetáculo em torno de questões de arte e cultura popular, com exposição e exibição de e sobre essas questões. Já a Aula Show é um gênero de realização curta, que emerge no interior de outro, mais rotineiro, a aula, trazendo uma nova cenografia e destacando-se por seu tom informal e lúdico.
A CARACTERIZAÇÃO DO GÊNERO EXPOSIÇÃO ORAL NO CONTEXTO DAS PRÁTICAS DE LINGUAGEM NA ESCOLA
Authors: Goulart Cláudia
Published: October 2019

Dada a pouca atenção com que os gêneros orais são abordados na escola, o objetivo deste artigo é descrever e caracterizar um dos gêneros orais formais públicos mais utilizados na esfera escolar: a exposição oral em seminários. O foco principal deste texto é apresentar as características textuais desse gênero, segundo o que postula Travaglia (2007) de forma a identificar e distinguir seu objetivo sociocomunicativo, a estrutura composicional, o conteúdo temático, o estilo e as condições de produção. Essa caracterização é importante porque pode auxiliar professores na tarefa de levar os estudantes a se apropriarem de um gênero secundário, formal, cujas habilidades são desenvolvidas por meio de reflexões. Assim fazendo, os professores podem auxiliar os estudantes a assumirem o lugar do enunciador que o expositor deve ocupar, objetivo principal da exposição oral. O corpus analisado foi constituído por gravações em áudio e vídeo de estudantes do último ano do ensino fundamental (9º ano) e do ensino médio em atividades de exposição oral em seminário nos conteúdos de Língua Portuguesa/Literatura, Física, Biologia e Geografia. Os resultados evidenciam que a exposição oral é um gênero que os alunos não dominam e são necessárias intervenções didáticas que possibilitem a reflexão sobre a importância de se aliar os recursos linguísticos e prosódicos aos elementos multissemióticos que se interpenetram e se complementam no curso das exposições orais.
GÊNERO ORAL: LEILÃO
Authors: Fernandes Maria José da Silva , Cardoso Solange Aparecida Faria
Published: October 2019

Este trabalho apresenta a análise e descrição do gênero oral leilão, especificamente o leilão que ocorre em quermesses e o leilão de gado, em situação real de realização. Esse gênero é essencial em determinadas atividades sociais e desempenha uma função social importante na comunidade discursiva onde se realiza. Fundamentamo-nos, nessa análise, nos pressupostos teóricos da Linguística Textual, especialmente nos estudos de Travaglia (2007), que apontam para a necessidade de observar alguns critérios para a caracterização do gênero, tais como o objetivo e a função sociocomunicativa, o conteúdo temático, a estrutura composicional, as características da superfície linguística e as condições de produção. Partimos da hipótese de que, embora se trate de diferentes leilões, eles são do mesmo gênero e possuem características em comum. Para o desenvolvimento da pesquisa, primeiramente fizemos a coleta de dados por meio de gravações dos referidos leilões, em cidades de Minas Gerais e Tocantins. Em seguida, transcrevemos os dados, utilizando as convenções adotadas pelo PETEDI. A análise inicial foi feita separadamente, ou seja, fizemos a análise do leilão de quermesse e depois do leilão de gado. Os resultados mostram que o leilão realizado em quermesses e o leilão de gado apresentam muitas características semelhantes, principalmente em relação à superestrutura. Daí concluirmos que se trata de um mesmo gênero.
Analisa Awal Kekasaran Permukaan Hasil Produksi Mesin Cetak Tiga Dimensi Terhadap Pengaruh Variasi Grit Size Sand Paper
Authors: Hakim Rahman , Sutisna Nanang Ali
Published: October 2019

Hasil produksi cetak tiga dimensi tipe FDM (Fused Deposition Modeling) dengan menggunakan material PLA (Polylactid Acid) yang tergolong material polymer pada umumnya mempunyai permukaan yang kasar sehingga memerlukan pekerjaan akhir untuk membuat permukaanya lebih halus lagi. Tujuan percobaan ini ialah untuk mengetahui ukuran Grit Size mana saja yang bisa menghasilkan nilai kekasaran permukaan yang paling rendah atau dengan kata lain mempunyai nilai kehalusan permukaan yang paling tinggi. Salah satu caranya ialah dengan menggunakan kertas gosok dengan variasi tingkat Grit Size yang biasa ditampilkan pada kode angka pada label kertas gosok. Pada percobaan kali ini, kami menggunakan Grit Size dengan ukuran 1000CW, 1200CW dan 1500CW. Parameter uji pada mesin cetak tiga dimensi dengan merek Leapfrog Creatr HS ini menggunakan PLA berdiameter 1.75mm, dengan temperatur Nozzle dan Base Plate sebesar 230oC dan 30oC, serta mempunyai feedrate dan infill density masing-masing sebesar 70%. Setelah dilakukan percobaan sebanyak tiga kali pengambilan data, kami dapatkan hasil yang dimana dengan menggunakan sand paper dengan grit size 1500CW mempunyai nilai kekasaran permukaan (Ra) yang paling rendah, yaitu sebesar 4,392 μm (N8).
Pendinginan Pengelasan dengan Metode SMAW pada Kekerasan Baja Karbon ST37 dengan Media Serbuk Semen Abu-Abu pada Beban Rockwell 100 kgf
Authors: Anhar Muh
Published: October 2019

Pengelasan merupakan bagian tak terpisahkan dari pertumbuhan peningkatan industri karena memegang peran utama dalam rekayasa dan reparasi produksi logam. Penelitian ini bertujuan untuk mengetahui pengaruh media pendingin terhadap kekerasan logam las dan logam HAZ (heat Affected Zone) yang diuji kekerasan menggunakan metode Rockwell. Penelitian ini dilakukan pada material baja karbon rendah ST 37 yang dilas menggunakan elektroda E6013 diameter 2,6 mm dengan sambungan tumpul, kemudian diberikan pendinginan menggunakan serbuk semen abu-abu. Logam las yang didinginkan dengan media serbuk semen putih memiliki kekerasan yang lebih keras dibandingkan dengan media serbuk semen abu-abu, semen abu-abu mengandung C3S yang memiliki kadar sulit terhidrasi dan agak panas.Setelah dilakukan pengujian pada beberapa titik, hasil berupa nilai rata-rata dari spesimen raw material adalah 48,3 HRB, pengelasan tanpa media isolator = 42,96 HRB dan HAZ = 46,4 HRB. Spesimen las dengan media serbuk semen abu – abu = 41,5 HRB dan HAZ = 43,45 HRB. Dilihat dari fenomena tersebut semen abu-abu memiliki nilai kekerasan yang lebih rendah dari pada udara, dimana HAZ pada kekerasan semen abu-abu memiliki nilai 43,45 HRB sedangkan nilai pada udara 46,4 HRB.
Cellular and physiological responses to drought stress in Aegilops tauschii genotypes
Authors: Falaknaz Mehran , Alaami Ali , Mehrabi Aliashraf , Sabouri Atefeh , Kahrizi Danial , Karimi Naser
Published: October 2019

Drought stress is one of the most important limiting factors in crop yield through impact on the cellular and physiological functions of the plant. Therefore, the study of physiological responses of plants can help to better understanding the drought tolerance mechanisms. In this experiment, 125 wild diploid wheat genotypes of Aegilops tauschii were evaluated for the physiological responses under rainfed and supplemental irrigation conditions. The physiological characteristics such as leaf relative water content (RWC), excised leaf water retention (ELWR), relative water loss (RWL), chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, ion leakage, membrane stability index (MSI) and proline content were measured. The results showed that the higher proline content, lower chlorophyll degradation rate and low amount of the membrane stability index (MSI) may inhibit the grain yield reduction under rainfed conditions. It was also found that the lower ion leakage due to the low cell membrane damage may led to the higher yield under rain-fed conditions. The results of regression analysis in both rainfed and supplemental irrigation conditions showed that proline content and total chlorophyll were introduced into the model, and explained the most variation in the grain yield. So, considering the above traits, the genotypes 16, 22, 43, 66 and 106 seems to be more drought tolerant and could be exploited in wheat breeding programs after further assessments.
Dietary fat intake and risk of bladder cancer: Evidence from a meta-analysis of observational studies
Authors: Wang Jian , Wang Chuanli
Published: October 2019

The association between dietary fat intake and bladder cancer had been inconsistent in the previous epidemiological studies. The aim of this study was to investigate the difference between fat intake and bladder cancer risk. Databases of PubMed, Embase, and Web of Science were systematically searched for suitable studies from inception to June 2018. A meta-analysis was performed to analyze the efficacy of dietary fat intake on bladder cancer risk. A Forest plot was prepared to indicate the relationship. Ten citations were used in this study. The Funnel plot suggested highest category of dietary fat intake could increase the risk of bladder cancer (summarized relative risk (RR)= 1.279, 95% confidence interval (CI)= 1.036-1.577, I2= 53.2%, P for heterogeneity = 0.019). A positive association was found among European populations (summarized RR= 1.359, 95%CI= 1.027-1.798), but not in North American populations. The association was not significant in the subgroup analysis by fat type on bladder cancer risk. Egger test (P= 0.239) and Funnel plot showed there was no significant publication bias in the included publications. In conclusions, compared with the lowest category of dietary fat intake, the highest category could significantly increase the bladder cancer risk, especially among European populations. As some limitations existed in our analysis, large scale studies with detailed amount of dietary fat intake are needed to verify our results.
Mesenchymal stem cell-derived exosomes improve diabetes mellitus-induced myocardial injury and fibrosis via inhibition of TGF-Î²1/Smad2 signaling pathway
Authors: Lin Yuan , Zhang Fang , Lian Xiao-fen , Peng Wei-qun , Yin Chao-yao
Published: October 2019

The aim of this study is to investigate  the effect of mesenchymal stem cell (MSC)-derived exosomes on diabetes mellitus-induced myocardial injury, and the underlying mechanism. Thirty adult male Sprague Dawley rats were randomly assigned to three groups of ten rats each: normal control group, diabetic control group and MSC exosomes group. Exosomes were isolated from MSCs through gradient ultracentrifugation. With the exception of normal control, diabetes mellitus (DM) was induced in the rats with a single intraperitoneal injection of 30 mg/kg body weight streptozotocin (STZ) in 0.1 mol/L sodium citrate buffer. Rats in MSC exosomes group were intravenously injected with MSC-derived exosomes once a week for 12 weeks. Left ventricular collagen (LVC) level was measured using acid hydrolysis method. Fatty acid transporters (FATPs) and fatty acid beta oxidase (FA-Î²-oxidase) were determined using enzyme-linked immunosorbent assay (ELISA). Gene and protein expressions of TGF-Î² and Smad2 were determined using real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting. Flow cytometric analysis and Western blotting revealed positive expression of exosomal specific marker, CD63. The level of LVC was significantly higher in diabetic control group than in normal control group, but was significantly reduced after treatment with MSC-derived exosomes (p < 0.05). The levels of FATPs and FA-Î²-oxidase were significantly lower in diabetic control group than in normal control group (p < 0.05). However, treatment with MSC-derived exosomes significantly increased the levels of these proteins (p < 0.05). The levels of expression of TGF-Î²1 and Smad2 mRNAs were significantly higher in the diabetic control group than in normal control group, but were significantly reduced after treatment with MSC-derived exosomes (p < 0.05). The expressions of TGF-Î²1 and Smad2 proteins were also significantly upregulated in diabetic control group, when compared with normal control group (p < 0.05). However, treatment with MSC-derived exosomes significantly down-regulated the expression of these proteins (p < 0.05). The results obtained in this study indicate that MSC-derived exosomes improve DM-induced myocardial injury and fibrosis via inhibition of TGF-Î²1/Smad2 signaling pathway.
Regulation of signaling pathways by Ampelopsin (Dihydromyricetin) in different cancers: exploring the highways and byways less travelled
Authors: Fayyaz Sundas , Qureshi Muhammad Zahid , Alhewairini Saleh S , Avnioglu Seda , Attar Rukset , Sabitaliyevich Uteuliyev Yerzhan , Buha Aleksandra , Salahuddin Hina , Adylova Aima , Tahir Fatima , Pawlak-Adamska Edyta
Published: October 2019

Ampelopsin or Dihydromyricetin is gradually emerging as a high-quality natural product because of its ability to modulate wide-ranging signaling pathways. Ampelopsin (Dihydromyricetin) has been reported to effectively modulate growth factor receptor (VEGFR2 and PDGFRÎ²) mediated signaling,  TRAIL/TRAIL-R pathway, JAK/STAT and mTOR-driven signaling in different cancers. Ampelopsin (Dihydromyricetin) has also been shown to exert inhibitory effects on the versatile regulators which trigger EMT (Epithelial-to-Mesenchymal Transition). Findings obtained from in-vitro studies are encouraging and there is a need to comprehensively analyze how Ampelopsin (Dihydromyricetin) inhibits tumor growth in different cancer models. Better knowledge of efficacy of Ampelopsin (Dihydromyricetin) in tumor bearing mice will be helpful in maximizing its translational potential.