bluuebunny/crossref_metadata_2025 huggingface dataset:
Metabolomic Biomarkers in Anxiety Disorders
Authors: Humer Elke , Pieh Christoph , Probst Thomas
Published: July 2020

Anxiety disorders range among the most prevalent psychiatric disorders and belong to the leading disorders in the study of the total global burden of disease. Anxiety disorders are complex conditions, with not fully understood etiological mechanisms. Numerous factors, including psychological, genetic, biological, and chemical factors, are thought to be involved in their etiology. Although the diagnosis of anxiety disorders is constantly evolving, diagnostic manuals rely on symptom lists, not on objective biomarkers and treatment effects are small to moderate. The underlying biological factors that drive anxiety disorders may be better suited to serve as biomarkers for guiding personalized medicine, as they are objective and can be measured externally. Therefore, the incorporation of novel biomarkers into current clinical methods might help to generate a classification system for anxiety disorders that can be linked to the underlying dysfunctional pathways. The study of metabolites (metabolomics) in a large-scale manner shows potential for disease diagnosis, for stratification of patients in a heterogeneous patient population, for monitoring therapeutic efficacy and disease progression, and for defining therapeutic targets. All of these are important properties for anxiety disorders, which is a multifactorial condition not involving a single-gene mutation. This review summarizes recent investigations on metabolomics studies in anxiety disorders.
Electrical and Magnetodielectric Properties of Magneto-Active Fabrics for Electromagnetic Shielding and Health Monitoring
Authors: Bunoiu Madalin , Anitas Eugen Mircea , Pascu Gabriel , Chirigiu Larisa Marina Elisabeth , Bica Ioan
Published: July 2020

An efficient, low-cost and environmental-friendly method to fabricate magneto-active fabrics (MAFs) based on cotton fibers soaked with silicone oil and iron oxide microfibers (mFe) at mass fractions 2 wt.%, 4 wt.% and 8 wt.% is presented. It is shown that mFe induce good magnetic properties in MAFs, which are subsequently used as dielectric materials for capacitor fabrication. The electrical properties of MAFs are investigated in a static magnetic field with intensities of 0 kA/m, 160 kA/m and 320 kA/m, superimposed on a medium-frequency electric field. The influence of mFe on the electrical capacitance and dielectric loss tangent is determined, and it can be observed that the electrical conductivity, dielectric relaxation times and magnetodielectric effects are sensibly influenced by the applied magnetic and electric fields. The results indicate that the MAFs have electrical properties which could be useful for protection against electromagnetic pollution or for health monitoring.
Hypoxia Promotes Prostate Cancer Aggressiveness by Upregulating EMT-Activator Zeb1 and SK3 Channel Expression
Authors: Bery Fanny , Figiel Sandy , Kouba Sana , Fontaine Delphine , Guéguinou Maxime , Potier-Cartereau Marie , Vandier Christophe , Guibon Roseline , Bruyère Franck , Fromont Gaëlle , Mahéo Karine
Published: July 2020

Hypoxia is a well-established feature of prostate cancer (PCa) and is associated with disease aggressiveness. The hypoxic microenvironment initiates multiple adaptive responses including epithelial-to-mesenchymal transition (EMT) and a remodeling of calcium homeostasis involved in cancer progression. In the present study, we identified a new hypoxia signaling pathway with a positive feedback loop between the EMT transcription factor Zeb1 and SK3, a Ca2+-activated K+ channel, which leads to amplifying store-operated Ca2+ entry. Zeb1 and SK3 channel were strongly upregulated by hypoxia both in vitro and ex vivo in organotypic cultures of human PCa. Taking into account the sensitivity of the SK3 channel to the membrane lipid composition, we identified lipids such as Ohmline (an alkyl ether lipid and SK3 inhibitor), linoleic acid (LA) and eicosapentaenoic acid (EPA) (fatty acids associated with indolent PCa), which were able to completely abrogate the hypoxia-induced changes in Zeb1 expression. Ultimately, better understanding of this new hypoxia-induced EMT pathway may allow to develop adjuvant therapeutic strategies, in order to control PCa aggressiveness and improve treatment outcomes.
Establishing Pompe Disease Newborn Screening: The Role of Industry
Authors: Keutzer Joan M.
Published: July 2020

When clinical trials for enzyme replacement therapy for Pompe disease commenced, a need for newborn screening (NBS) for Pompe disease was recognized. Two methods for NBS for Pompe disease by measuring acid α-glucosidase in dried blood spots on filter paper were developed in an international collaborative research effort led by Genzyme. Both methods were used successfully in NBS pilot programs to demonstrate the feasibility of NBS for Pompe disease. Since 2009, all babies born in Taiwan have been screened for Pompe disease. Pompe disease was added to the Recommended Uniform (Newborn) Screening Panel in the United States in 2015. NBS for Pompe disease is possible because of the unprecedented and selfless collaborations of countless international experts who shared their thoughts and data freely with the common goal of establishing NBS for Pompe disease expeditiously.
Effect of Limestone Powder and Fine Gypsum on the Cracking Tendency of Blast-Furnace Slag Cement Concrete Subjected to Accelerated Curing
Authors: Zulu Bernard A. , Miyazawa Shingo , Nito Nobukazu
Published: July 2020

Thermal stresses are generated in concrete during the accelerated curing process in precast concrete. These stresses may cause concrete to crack, which would have a negative effect on the general concrete performance. This paper provides the results of the thermal stress analysis of concrete containing 25% replacement of ordinary Portland cement (OPC) by ground granulated blast-furnace slag cement (GGBFS) with 3000 cm2/g fineness. A total of 2.5% of limestone powder and 1.75% of fine gypsum by mass of OPC were incorporated in the other concrete mix proportion to check their effect on the cracking tendency of concrete. The concrete was subjected to heat curing. JCMAC, a 3D finite element analysis software developed in Japan, was used in this study for the thermal stress analysis. The heat curing period of one day, similar to generally used in the precast concrete industry for civil engineering products consisting of 3 h preheating period, heating to a peak temperature of 65 °C and this temperature was kept for 3 h, and lastly cooling was used in the study. A standard precast box culvert model member together with some experimental results obtained in the laboratory were used in the analysis. Lower cracking resistance at 1 day was observed in both mix proportions that would lead to cracking at demolding time. Curing sheets were then introduced in the analysis to cover the precast mold during accelerated curing and this showed improvement in cracking resistance of concrete containing limestone powder and fine gypsum.
Rapid Chairside Microbial Detection Predicts Endodontic Treatment Outcome
Authors: Knight Alan , Blewitt Ian , Al-Nuaimi Nassr , Watson Tim , Herzog Dylan , Festy Frederic , Patel Shanon , Foschi Federico , Koller Garrit , Mannocci Francesco
Published: July 2020

Background. The aim of this longitudinal, one-year cohort study was to explore the hypothesis that fluorescence sampling of the root canal space prior to obturation could predict the outcome of root canal treatment (RCT). Methods. Sixty-five teeth underwent primary RCT and were followed up clinically and radiographically. The outcome was determined radiographically with periapical radiographs (PR) and cone beam computed tomography (CBCT) scans. Results. Success at 12 months was predictable based on the fluorescence score. When the fluorescence score (defined as the percentage of signal over total signal including background) was lower than 67, there was a 4.5 times (Odds ratio (OR) = 0.028; 95% confidence interval (CI): 0.003, 0.291, p = 0.001) greater chance of success (90% overall). When the readings were above this threshold, the success rate was 20%. Conclusion. A chairside sampling method is able to predict the outcome of RCT, through the use of paper point sampling and fluorescence staining. This has reduced the prevalence of persistent infections by guiding the optimum time for obturation. ClinicalTrials.gov trial NCT03660163.
Hepatitis C Virus Influences HIV-1 Viral Splicing in Coinfected Patients
Authors: Martínez-Román Paula , López-Huertas María Rosa , Crespo-Bermejo Celia , Arca-Lafuente Sonia , Cortegano Isabel , Valle-Millares Daniel , Gaspar María Luisa , Martín-Carbonero Luz , Domínguez-Domínguez Lourdes , Ryan Pablo , de los Santos Ignacio , de la Fuente-Moral Sara , Fernández-Rodríguez Amanda , Coiras Mayte , Briz Verónica
Published: July 2020

Coinfection with hepatitis C virus (HCV) influences HIV reservoir size. However, it is unknown whether this coinfection also induces a higher provirus transcription. Viral transcription is promoted by synergy between cellular factors such as NF-κB and the viral regulator Tat. The impact of HCV coinfection on HIV provirus transcription was analyzed in resting (r)CD4 T+ cells (CD3+CD4+CD25-CD69-HLADR-) and rCD4 T cells-depleted PBMCs (rCD4 T- PBMCs) from a multicenter cross-sectional study of 115 cART-treated HIV patients: 42 HIV+/HCV+ coinfected individuals (HIV+/HCV+), 34 HIV+ patients with HCV spontaneous clearance (HIV+/HCV−) and 39 HIV patients (HIV+). Viral transcription was assessed in total RNA through the quantification of unspliced, single spliced, and multiple spliced viral mRNAs by qPCR. Linear correlations between viral reservoir size and viral splicing were determined. A 3-fold increase of multiple spliced transcripts in rCD4 T+ cells of HIV+/HCV+ patients was found compared to HIV+ individuals (p < 0.05). As Tat is synthesized by multiple splicing, the levels of Tat were also quantified in these patients. Significant differences in single and multiple spliced transcripts were also observed in rCD4 T- PBMCs. Levels of multiple spliced mRNAs were increased in rCD4 T+ cells isolated from HIV+/HCV+ subjects, which could indicate a higher Tat activity in these cells despite their resting state.
Comparative Analysis of Three Machine-Learning Techniques and Conventional Techniques for Predicting Sepsis-Induced Coagulopathy Progression
Authors: Hasegawa Daisuke , Yamakawa Kazuma , Nishida Kazuki , Okada Naoki , Murao Shuhei , Nishida Osamu
Published: July 2020

Sepsis-induced coagulopathy has poor prognosis; however, there is no established tool for predicting it. We aimed to create predictive models for coagulopathy progression using machine-learning techniques to evaluate predictive accuracies of machine-learning and conventional techniques. A post-hoc subgroup analysis was conducted based on the Japan Septic Disseminated Intravascular Coagulation retrospective study. We used the International Society on Thrombosis and Haemostasis disseminated intravascular coagulation (DIC) score to calculate the ΔDIC score as ((DIC score on Day 3) − (DIC score on Day 1)). The primary outcome was to determine whether the predictive accuracy of ΔDIC was more than 0. The secondary outcome was the actual predictive accuracy of ΔDIC (predicted ΔDIC−real ΔDIC). We used the machine-learning methods, such as random forests (RF), support vector machines (SVM), and neural networks (NN); their predictive accuracies were compared with those of conventional methods. In total, 1017 patients were included. Regarding DIC progression, predictive accuracy of the multiple linear regression, RF, SVM, and NN models was 63.7%, 67.0%, 64.4%, and 59.8%, respectively. The difference between predicted ΔDIC and real ΔDIC was 2.05, 1.54, 2.24, and 1.77 for the multiple linear regression, RF, SVM, and NN models, respectively. RF had the highest predictive accuracy.
The Use of Demoralization Scale in Italian Kidney Transplant Recipients
Authors: Battaglia Yuri , Zerbinati Luigi , Piazza Giulia , Martino Elena , Massarenti Sara , Provenzano Michele , Esposito Pasquale , Andreucci Michele , Storari Alda , Grassi Luigi
Published: July 2020

Demoralization is a commonly observed syndrome in medically ill patients. The risk of demoralization may increase in patients after a kidney transplant (KTRs) because of the stressful nature of renal transplantation, psychosocial challenges, and adjustment needs. No study is available on demoralization amongst KTRs. The purpose of our study was to evaluate the validity of the Italian version of the Demoralization Scale (DS-IT) and the prevalence of demoralization in KTRs. Also, we aimed at exploring the association of the DS-IT with International Classification of Diseases (ICD) psychiatric diagnoses, post-traumatic growth (PTG), psychological and physical symptoms, and daily-life problems. A total of 134 KTRs were administered the MINI International Neuropsychiatric Interview 6.0. and the Diagnostic Criteria for Psychosomatic Research–Demoralization (DCPR/D) Interview. The DS-IT, the Edmonton Symptom Assessment System (ESAS), the Canadian Problem Checklist (CPC), were used to measure demoralization, physical and psychological symptoms, and daily-life problems; also, positive psychological experience of kidney transplantation was assessed with the PTG Inventory. Routine biochemistry and sociodemographic data were collected. Exploratory factor analysis demonstrated a four-dimensional factor structure of the DS-IT, explaining 55% of the variance (loss of meaning and purpose, disheartenment, dysphoria, and sense of failure). DS-IT Cronbach alpha coefficients indicated good or acceptable level of internal consistency. The area under the Receiving Operating Characteristics (ROC) curve for DS-IT (against the DCPR/D interview as a gold standard) was 0.92. The DS-IT optimal cut-off points were ≥20 (sensitivity 0.87, specificity 0.82). By examining the level of demoralization, 14.2%, 46.3%, 24.6%, and 14.6% of our sample were classified as having no, low, moderate, and high demoralization, respectively, with differences according to the ICD psychiatric diagnoses (p < 0.001). DS-IT Total and subscales scores were positively correlated with scores of ESAS symptoms and CPC score. A correlation between DS-IT loss of meaning and purpose subscale and PTGI appreciation of life subscale (p < 0.05) was found. This study shows, for the first time, a satisfactory level of reliability of the DS-IT and a high prevalence of severe demoralization in KTRs.
Three-Dimensional Radiographic Outcome of Free-Handed Flaplessly Placed Mini Dental Implants in Edentulous Maxillae after 2-Years Function
Authors: Van Doorne Luc , Gholami Pedram , D’haese Jan , Hommez Geert , Meijer Gert , De Bruyn Hugo
Published: July 2020

Background: Free-handed, flaplessly placed mini dental implants (MDIs) are a valuable, more affordable and minimally invasive treatment to support overdentures in fully edentulous jaws, especially for medically compromised patients. However, critical 3D radiographic evaluation is lacking. This multicenter prospective case series assessed clinical outcome and carried out 3D- cone-beam computerized tomography (CBCT) analysis of free-handed flaplessly placed one-piece maxillary MDIs by an experienced maxillofacial surgeon. Methods: Thirty-one patients suffering from an ill-fitting maxillary denture relating to compromised bone volume (as confirmed on CBCT), with a dentate mandible, were selected. They received 5–6 MDIs free-hand flaplessly placed and mentally guided with preoperative CBCT. Final connection and attachment activation took place six months later. After two years each implant was individually assessed with CBCT for perforations on eight sites. Implant survival, prosthetic failure, clinical stability and sinus/nasal complaints were registered after three years. Results: 32/185 (17.3%) MDIs failed during the provisional loading with non-activated attachments; 17 replacements in 10 patients were performed. Of the 170 actively loaded 170 MDIs, 82.3% survived and 27/31 prostheses (87%) were fully functional. In total 98/170 MDIs showed no perforation. Based on 1360 CBCT observations, 231 perforations (16.9%) were registered. Of most perforations 37 (25%) were observed at the apical tip and 37 were positioned (21%) into the sinus/nasal cavity, although without clinical complaints. Conclusions: Given the compromised population, the minimally invasive procedure and the low treatment cost involved, a failure rate of 17% is substantial, however clinically acceptable given the critical bone condition. However, even in experienced surgical hands, freehanded and flapless placement yield a high risk for implant perforation, although this did not necessarily lead to complications.
Kidney Involvement in Hypocomplementemic Urticarial Vasculitis Syndrome—A Case-Based Review
Authors: Ion Oana , Obrișcă Bogdan , Ismail Gener , Sorohan Bogdan , Bălănică Sonia , Mircescu Gabriel , Sinescu Ioanel
Published: July 2020

Hypocomplementemic urticarial vasculitis syndrome (HUVS), or McDuffie syndrome, is a rare small vessel vasculitis associated with urticaria, hypocomplementemia and positivity of anti-C1q antibodies. In rare cases, HUVS can manifest as an immune-complex mediated glomerulonephritis with a membranoproliferative pattern of injury. Due to the rarity of this disorder, little is known about the clinical manifestation, pathogenesis, treatment response and outcome of such patients. We describe here three cases of HUVS with severe renal involvement. These patients had a rapidly progressive form of glomerulonephritis with severe nephrotic syndrome against a background of a membranoproliferative pattern of glomerular injury with extensive crescent formation. Therefore, these patients required aggressive induction and maintenance immunosuppressive therapy, with a clinical and renal response in two patients, while the third patient progressed to end-stage renal disease. Because of the rarity of this condition, there are few data regarding the clinical presentation, pathology and outcome of such patients. Accordingly, we provide an extensive literature review of cases reported from 1976 until 2020 and place them in the context of the current knowledge of HUVS pathogenesis. We identified 60 patients with HUVS and renal involvement that had adequate clinical data reported, out of which 52 patients underwent a percutaneous kidney biopsy. The most frequent renal manifestation was hematuria associated with proteinuria (70% of patients), while one third had abnormal kidney function on presentation (estimated glomerular filtration (GFR) below 60 mL/min/1.73 m2). The most frequent glomerular pattern of injury was membranoproliferative (35%), followed by mesangioproliferative (21%) and membranous (19%). Similar to other systemic vasculitis, renal involvement carries a poorer prognosis, but the outcome can be improved by aggressive immunosuppressive treatment.
Walking Speed is the Sole Determinant Criterion of Sarcopenia of Mild Cognitive Impairment in Japanese Elderly Patients with Type 2 Diabetes Mellitus
Authors: Machii Noritaka , Kudo Akihiro , Saito Haruka , Tanabe Hayato , Iwasaki Mariko , Hirai Hiroyuki , Masuzaki Hiroaki , Shimabukuro Michio
Published: July 2020

Diabetes mellitus is a risk factor for mild cognitive impairment (MCI) and dementia. However, how the clinical characteristics of MCI patients with type 2 diabetes mellitus are linked to sarcopenia and/or its criteria remain to be elucidated. Japanese patients with type 2 diabetes mellitus were categorized into the MCI group for MoCA-J (the Japanese version of the Montreal cognitive assessment) score <26, and into the non-MCI group for MoCA-J ≥26. Sarcopenia was defined by a low skeletal mass index along with low muscle strength (handgrip strength) or low physical performance (walking speed <1.0 m/s). Univariate and multivariate-adjusted odds ratio models were used to determine the independent contributors for MoCA-J <26. Among 438 participants, 221 (50.5%) and 217 (49.5%) comprised the non-MCI and MCI groups, respectively. In the MCI group, age (61 ± 12 vs. 71 ± 10 years, p < 0.01) and duration of diabetes mellitus (14 ± 9 vs. 17 ± 9 years, p < 0.01) were higher than those in the non-MCI group. Patients in the MCI group exhibited lower hand grip strength, walking speed, and skeletal mass index, but higher prevalence of sarcopenia. Only walking speed (rather than muscle loss or muscle weakness) was found to be an independent determinant of MCI after adjusting for multiple factors, such as age, gender, body mass index (BMI), duration of diabetes mellitus, hypertension, dyslipidemia, smoking, drinking, estimated glomerular filtration rate (eGFR), HbA1c, and history of coronary heart diseases and stroke. In subgroup analysis, a group consisting of male patients aged ≥65 years, with BMI <25, showed a significant OR for walking speed. This study showed that slow walking speed is a sole determinant criterion of sarcopenia of MCI in patients with type 2 diabetes mellitus. It was suggested that walking speed is an important factor in the prediction and prevention of MCI development in patients with diabetes mellitus.
Fabrication and Characterization of Flexible Three-Phase ZnO-Graphene-Epoxy Electro-Active Thin-Film Nanocomposites: Towards Applications in Wearable Biomedical Devices
Authors: Singh Mandeep , Kumar Sanjeev , Zoghi Shervin , Cervantes Yerli , Sarkar Debaki , Ahmed Saquib , Chowdhury Shaestagir , Banerjee Sankha
Published: July 2020

Perovskite oxides have been used as sensors, actuators, transducers, for sound generation and detection, and also in optical instruments and microscopes. Perovskite halides are currently considered as optoelectronic devices such as solar cells, photodetectors, and radiation detection, but there are major issues with stability, interfacial recombination, and electron/hole mobility. The following work looks into the fabrication of non-toxic ZnO-based lead-free alternatives to perovskite oxides for use as secondary sensors or electron transport layers along with perovskite halides for application in stacked biomedical wearable devices. Three-phase, lead-free, Zinc Oxide-Graphene-Epoxy electroactive nanocomposite thin films were fabricated. The volume fraction of the Graphene phase was held constant at 10%, while the volume fraction of the ZnO phase was varied from 10–70%. The dielectric constant, capacitance, impedance, resistance, and conductance of the samples were measured using an impedance analyzer, and the results were compared as a function of volume fraction of ZnO to understand the electron transport performance of these thin films. The impedance and dielectric spectra of the nanocomposites were recorded over a frequency range of 20 Hz to 10 MHz. The microstructural properties and cross-section of the thin films were analyzed using a Scanning Electron Microscope. The high sensitivity and electron transport properties of the composite could be potentially utilized in biomedical devices at low- and high-frequency ranges.
Role of Hepcidins from Black Rockfish (Sebastes schlegelii) in Iron-Metabolic Function and Bacterial Defense
Authors: Ma Yunqi , Lee Chang-Joo , Kim So-Sun , Kim David Nahm-Joon , Nam Bo-Hye , Kim Young-Ok , An Cheul-Min , Park Jang-Su
Published: July 2020

Hepcidin, an antimicrobial peptide produced by the liver, also controls the iron balance and regeneration in vertebrates. Two types of hepcidin (Hamp1 and Hamp2) have been found in the bodies of black rockfish (Sebastes schlegelii). The full-length cDNA of hepcidin was cloned to enable a study of the antibacterial roles of these two hepcidins (Hamp) in black rockfish. The antimicrobial function of recombinant hepcidins was tested both in vitro and in vivo by the synthesis in Escherichia coli of recombinant hepcidin (approximately 11 kDa) from black rockfish. The recombinant hepcidins inhibited the growth of two bacterial species, Streptococcus iniae FP5228 and Pseudomonas aeruginosa, at various concentrations, in vitro after 6 h post-incubation, respectively. During infection, the production of ferroportin was reduced, suggesting the preservation of iron to prevent microbial proliferation. In vivo administration of Hamp1, but not Hamp2, synthetic peptides induced a substantial reduction in the expression of ferroportin, suggesting that in black rockfish with two forms of hepcidin, ferroportin production is regulated by the iron-regulator Hamp1, and not by the dedicated antimicrobial Hamp2. The findings of this study suggest the various antimicrobial roles of these two types of hepcidin.
IMO Second Generation Intact Stability Criteria: General Overview and Focus on Operational Measures
Authors: Petacco Nicola , Gualeni Paola
Published: July 2020

At the beginning of 2020, after a long and demanding process, the Second Generation Intact Stability criteria (SGISc) have been finalized at the 7th session of the International Maritime Organization (IMO) sub-committee on Ship Design and Construction (SDC). At present, SGISc are not mandatory, nevertheless IMO endorses their application in order to assess their consistency and validity. It is envisaged that SGISc can support the design of safer ships, nevertheless such a rules framework might have an impact also on the ship operational aspects in a seaway. In fact, within the SGISc framework, Operational Measures have also been implemented providing guidance and limitations during navigation. After a comprehensive overview about SGISc vulnerability levels and direct stability assessment, this paper provides a specific insight into the methodological approach for the Operational Measures extensively addressed as a complementary action to ship design.
An Empirical Investigation on Determinants of Sustainable Economic Growth. Lessons from Central and Eastern European Countries
Authors: Ioan Batrancea , Mozi Rathnaswamy Malar , Lucian Gaban , Gheorghe Fatacean , Horia Tulai , Ioan Bircea , Mircea-Iosif Rus
Published: July 2020

The study focuses on the effects of imports, exports, financial direct investment inflow and financial direct investment outflow on sustainable economic growth expressed by various macroeconomic indicators (gross domestic product, gross domestic savings, gross domestic capital) using the least squares panel method. Sample data were selected for ten Central and Eastern European (CEE) countries and the time frame considered was 2005–2016. Generally, transitional economies have to incorporate strong savings and a steady capital formation in order to achieve higher economic growth via foreign direct investment. Results showed that the analyzed factors played a major role in the sustainable economic growth of CEE countries. Another important and valuable insight of this study is that the financial sector steers the process of achieving sustainable economic growth across CEE countries.
Towards a Correlation between Structural, Magnetic, and Luminescence Properties of CeF3:Tb3+ Nanocrystals
Authors: Bartha Cristina , Secu Corina , Matei Elena , Negrila Catalin , Leca Aurel , Secu Mihail
Published: July 2020

In this study, we report on the structural, magnetic, and optical properties of Tb3+-doped CeF3 nanocrystals prepared via a polyol-assisted route, followed by calcination. X-ray diffraction analysis and electron microscopy investigations have shown the formation of a dominant Ce0.75F3 nanocrystalline phase (of about 99%), with a relatively uniform distribution of nanocrystals about 15 nm in size. Magnetization curves showed typical paramagnetic properties related to the presence of Ce3+ and Tb3+ ions. The magnetic susceptibility showed a weak inflexion at about 150 K, assigned to the cerium ions’ crystal field splitting. Under UV light excitation of the Ce3+ ions, we observed Tb3+ green luminescence with a quantum yield of about 20%.
Control of Particle Size in Flame Spray Pyrolysis of Tb–doped Y2O3 for Bio-Imaging
Authors: Khan Sovann , Choi Yunseok , Ahn Hak-Young , Han Jae Hyun , Ju Byeong-Kwon , Chung Jaewon , Cho So-Hye
Published: July 2020

Recently, the use of oxide-based nanomaterials for bio-imaging has received great attention owing to their remarkable stabilities as compared to those of conventional organic dyes. Therefore, the development of scalable methods for highly luminescent oxide materials with fine control of size has become crucial. In this study, we suggested modified flame spray pyrolysis (FSP) as a scalable method to produce a green-light emitting phosphor—Tb–doped Y2O3—in the nanometer size range. In our FSP method, an alkali salt (NaNO3) was found to be highly effective as a size-controlling agent when it is simply mixed with other metal nitrate precursors. The FSP of the mixture solution resulted in oxide composites of Y2O3:Tb3+ and NaxO. However, the sodium by-product was easily removed by washing with water. This salt-assisted FSP produced nano-sized and well-dispersed Y2O3:Tb3+ nanoparticles; their crystallinity and luminescence were higher than those of the bulk product made without the addition of the alkali salt. The nanoparticle surface was further coated with silica for biocompatibility and functionalized with amino groups for the attachment of biological molecules.
Use of Recycled Fine Aggregates from C&DW for Unbound Road Sub-Base
Authors: Courard Luc , Rondeux Mélanie , Zhao Zengfeng , Michel Frédéric
Published: July 2020

Fine recycled aggregates are produced in large quantities when crushing Construction and Demolition Waste (C&DW). Even if coarse recycled aggregates are commonly used for road foundations, fine particles are often rejected as they are considered detrimental for the long-term behaviour of foundations. Physicochemical, mineralogical and mechanical characterizations (through X-ray diffraction, X-ray fluorescence, the chloride and sulphate contents, Los Angeles abrasion, micro-Deval resistance and static plate load tests) were performed on raw and treated fine recycled materials for understanding both the effects of the preparation, the compaction and the freeze–thaw cycles on the properties and the evolution of fine particles. Special attention was provided to the shape analysis of fines by means of image analyser. The results showed that the main characteristic parameters to be considered are the sieving curve and the proportion of grades. The mixes containing the highest quantity of fine particles, specifically lower than 63 µm, usually inducing a higher water demand and a higher capillary rise. This can be explained by specific surface and bluntness parameters which increase with the finer particles, inducing a higher surface roughness and, consequently, a higher potential interaction with water. Compaction did not seem to have a major effect on the production of fines (despite some breakdown occurred during compaction) and on the shape of materials (the bluntness and convexity increased slightly, while the elongation values remained similar after the compaction process). The static plate load tests showed that bearing capacity is slightly lower than the specifications for the road foundation after compaction. However, the studied material could meet the maximum criteria for secondary roads foundation construction on the wear resistance criteria. Recycled aggregates from C&DW without sufficient quality could be blended with other aggregates to enable their usage for upper-level road foundation.
Design and Fabrication of New High Entropy Alloys for Evaluating Titanium Replacements in Additive Manufacturing
Authors: Sarswat Prashant , Smith Taylor , Sarkar Sayan , Murali Arun , Free Michael
Published: July 2020

High entropy alloys (HEAs) were prepared using the powder bed fusion (PBF) technique. Among titanium free alloys AlCoCrFeNiMn, CoCr1.3FeMnNi0.7, AlCoCrFeNi1.3, and AlCoCr1.3FeNi1.3 have been further investigated. A cost comparison was done for these four alloys as well as the titanium-based alloys AlCoCrFeNiTi and AlCo0.8CrFeNiTi. Such a comparison was done in order to evaluate the performance of the titanium-free alloys as the estimated cost of these will be less than for Ti-based HEAs. Hence, we have chosen four titanium free alloys and two titanium-based alloys for further processing. All these alloys were fabricated and subsequently characterized for phase, purity and performance. Scanning electron microscopy-based images were captured for microstructure characterization. EIS-based tests and potentiodynamic scans were performed to evaluate corrosion current. Hardness tests were performed for mechanical properties evaluation. Additional testing using factorial design tests was performed to evaluate the effects of various parameters to create better PBF-based HEA samples. EBSD tests, accelerated corrosion tests (mass loss), chemical analysis after degradation, microstructure analysis before and after degradation, and mechanical property comparison for finalized samples and other similar tests were executed. The details about all these HEAs and subsequent laser processing as well as behavior of these HEAs have been included in this study. It has been observed that some of the selected alloys exhibit good performance compared to Ti-based alloys, especially with respect to improvements in elastic constant and hardness relative to commercially pure Ti.
The Influence of the Layer Arrangement on the Distortional Post-Buckling Behavior of Open Section Beams
Authors: Kubiak Tomasz , Urbaniak Mariusz , Kazmierczyk Filip
Published: July 2020

The paper deals with the design of the stacking sequence of layers in the laminate beams with open-cross sections in order to create the desired behavior in the post-buckling range. Laminate beams with channel and lipped channel cross-sections made of glass fiber reinforced polymer (GFRP) laminate with different layer arrangements (symmetrical and nonsymmetrical) have been considered. In case of the nonsymmetrical stacking sequences, hygro-thermally curvature stable (HTCS) laminates have been taken into account. Pure bending was assumed as the type of load. In the case of beams with open cross-sections, this load type can cause the lateral-distortional buckling mode. A parametric study was performed to analyze the influence of layer arrangement on post-buckling behavior. The finite element method was used to developed numerical models and conduct simulations. Additionally, the experimental tests of the channel section beams were performed in order to validate the developed numerical models.
Experimental Study on Mechanical and Functional Properties of Reduced Graphene Oxide/Cement Composites
Authors: Zhang Ning , She Wei , Du Fengyin , Xu Kaili
Published: July 2020

This study develops a novel self-sensing cement composite by simply mixing reduced graphene oxide (rGO) in cementitious material. The experimental results indicate that, owing to the excellent dispersion method, the nucleation and two-dimensional morphological effect of rGO optimizes the microstructure inside cement-based material. This would increase the electric conductivity, thermal property and self-induction system of cement material, making it much easier for cementitious material to better warn about impending damage. The use of rGO can improve the electric conductivity and electric shielding property of rGO-paste by 23% and 45%. The remarkable enhancement was that the voltage change rate of 1.00 wt.%-rGO paste under six-cycle loads increased from 4% to 12.6%, with strain sensitivity up to 363.10, without compromising the mechanical properties. The maximum compressive strength of the rGO-mortar can be increased from 55 MPa to 71 MPa. In conclusion, the research findings provide an effective strategy to functionalize cement materials by mixing rGO and to achieve the stronger electric shielding property and higher-pressure sensitivity of rGO–cement composites, leading to the development of a novel high strength self-sensing cement material with a flexural strength up to 49%.
Two Forms of the Integral Representations of the Mittag-Leffler Function
Authors: Saenko Viacheslav
Published: July 2020

The integral representation of the two-parameter Mittag-Leffler function E ρ , μ ( z ) is considered in the paper that expresses its value in terms of the contour integral. For this integral representation, the transition is made from integration over a complex variable to integration over real variables. It is shown that as a result of such a transition, the integral representation of the function E ρ , μ ( z ) has two forms: the representation “A” and “B”. Each of these representations has its advantages and drawbacks. In the paper, the corresponding theorems are formulated and proved, and the advantages and disadvantages of each of the obtained representations are discussed.
B-Learning in Basic Vocational Training Students for the Development of the Module of Applied Sciences I
Authors: Hinojo-Lucena Francisco-Javier , Trujillo-Torres Juan-Manuel , Marín-Marín José-Antonio , Rodríguez-Jiménez Carmen
Published: July 2020

Information and communication technologies are a step forward in education, as they have given rise to innovative methodologies, such as blended learning. This type of training can be applied at any stage or educational typology such as basic vocational training. The main objective of this article is to know the degree of effectiveness of this methodology in this stage, specifically in an applied science module. For this purpose, a quasi-experimental design has been applied with a control group and an experimental group with a total of 147 participants. The results show how those students who have worked through b-learning have experienced better results in all the dimensions of the study. In conclusion, the implementation of this methodology in basic vocational training brings benefits, such as motivation and autonomy in the teaching–learning processes of all students.
Osirisynes G-I, New Long-Chain Highly Oxygenated Polyacetylenes from the Mayotte Marine Sponge Haliclona sp.
Authors: Campos Pierre-Eric , Herbette Gaëtan , Chendo Christophe , Clerc Patricia , Tintillier Florent , de Voogd Nicole J. , Papanagnou Eleni-Dimitra , Trougakos Ioannis P. , Jerabek Moran , Bignon Jérôme , Le Goff Géraldine , Ouazzani Jamal , Gauvin-Bialecki Anne
Published: July 2020

Chemical study of the CH2Cl2−MeOH (1:1) extract from the sponge Haliclona sp. collected in Mayotte highlighted three new long-chain highly oxygenated polyacetylenes, osirisynes G-I (1–3) together with the known osirisynes A (4), B (5), and E (6). Their structures were elucidated by 1D and 2D NMR spectra and HRESIMS and MS/MS data. All compounds were evaluated on catalase and sirtuin 1 activation and on CDK7, proteasome, Fyn kinase, tyrosinase, and elastase inhibition. Five compounds (1; 3–6) inhibited proteasome kinase and two compounds (5–6) inhibited CDK7 and Fyn kinase. Osirisyne B (5) was the most active compound with IC50 on FYNB kinase, CDK7 kinase, and proteasome inhibition of 18.44 µM, 9.13 µM, and 0.26 µM, respectively.
Comparison of Different Manufacturing Processes of AISI 4140 Steel with Regard to Surface Modification and Its Influencing Depth
Authors: Borchers Florian , Clausen Brigitte , Eckert Sandro , Ehle Lisa , Epp Jeremy , Harst Simon , Hettig Matthias , Klink Andreas , Kohls Ewald , Meyer Heiner , Meurer Markus , Rommes Bob , Schneider Sebastian , Strunk Rebecca
Published: July 2020

The surface and subsurface conditions of components are significant for their functional properties. Every manufacturing process step changes the surface condition due to its mechanical, chemical and/or thermal impact. The depth of the affected zone varies for different machining operations, and is predetermined by the process parameters and characteristics. Furthermore, the initial state has a decisive influence on the interactions that lead to the final surface conditions. The aim of the investigation presented here is to compare the influence of the load characteristics over the depth applied to manufactured components by several different machining operations and to determine the causing mechanisms. In order to ensure better comparability between the surface modifications caused by different machining operations, the same material was used (AISI 4140; German steel grade 42CrMo4 acc. to DIN EN 10083-3) and annealed to a ferritic-pearlitic microstructure. Based on interdisciplinary cooperation within the collaborative research center CRC/Transregio 136 “Process Signatures”, seven different manufacturing processes, i.e., grinding, turning, deep rolling, laser processing, inductive heat treatment, electrical discharge machining (EDM) and electrochemical machining (ECM), were used, and the resulting surface zones were investigated by highly specialized analysis techniques. This work presents the results of X-ray measurements, hardness measurements and electron microscopic investigations. As a result, the characteristics and depths of the material modifications, as well as their underlying mechanisms and causes, were studied. Mechanisms occurring within 42CrMo4 steel due to thermal, mechanical, chemical or mixed impacts were identified as phase transformation, solidification and strengthening due to dislocation generation and accumulation, continuum dynamic recrystallization and dynamic recovery, as well as chemical reactions.
Role of Vanadium Additions on the Corrosion Mitigation of Ti-6Al-xV Alloy in Simulated Body Fluid
Authors: Sherif El-Sayed M. , Ragab Sameh A. , Abdo Hany S.
Published: July 2020

The manufacturing of different Ti-6Al-xV (x = 2, 4, 6, and 8 wt.%) alloys using a mechanical alloying technique was reported. The corrosion behaviors of these newly fabricated alloys after 1, 24, and 48 h exposure to a simulated body fluid (SBF) were assessed using cyclic potentiodynamic polarization, electrochemical impedance spectroscopy, and chronoamperometric measurements. Surface morphology and elemental analyses after corrosion for 48 h in SBF were reported using scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) examinations. An X-ray diffraction investigation characterized the phase analyses. All results indicated that the increase of V content significantly decreases both uniform and pitting corrosion. This effect also increases with prolonging the immersion time to 48 h before measurement.
Prediction of the Bilinear Stress-Strain Curve of Aluminum Alloys Using Artificial Intelligence and Big Data
Authors: Merayo Fernández David , Rodríguez-Prieto Alvaro , Camacho Ana María
Published: July 2020

Aluminum alloys are among the most widely used materials in demanding industries such as aerospace, automotive or food packaging and, therefore, it is essential to predict the behavior and properties of each component. Tools based on artificial intelligence can be used to face this complex problem. In this work, a computer-aided tool is developed to predict relevant mechanical properties of aluminum alloys—Young’s modulus, yield stress, ultimate tensile strength and elongation at break. These predictions are based on the alloy chemical composition and tempers, and are employed to estimate the bilinear approximation of the stress-strain curve, very useful as a decision tool that helps in the selection of materials. The system is based on the use of artificial neural networks supported by a big data collection about technological characteristics of thousands of commercial materials. Thus, the volume of data exceeds 5 k entries. Once the relevant data have been retrieved, filtered and organized, an artificial neural network is defined and, after the training, the system is able to make predictions about the material properties with an average confidence greater than 95 % . Finally, the trained network is employed to show how it can be used to support decisions about engineering applications.
Paratuberculosis: A Potential Zoonosis and a Neglected Disease in Africa
Authors: Okuni Julius Boniface , Hansen Sören , Eltom Kamal H. , Eltayeb ElSagad , Amanzada Ahmad , Omega Joseph Amesa , Czerny Claus Peter , Abd El Wahed Ahmed , Ojok Lonzy
Published: July 2020

The Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of paratuberculosis, which is an economically important disease of ruminants. The zoonotic role of MAP in Crohn’s disease and, to a lesser extent, in ulcerative colitis, the two major forms of idiopathic inflammatory bowel disease (IIBD), has been debated for decades and evidence continues to mount in support of that hypothesis. The aim of this paper is to present a review of the current information on paratuberculosis in animals and the two major forms of IIBD in Africa. The occurrence, epidemiology, economic significance and “control of MAP and its involvement IIBD in Africa” are discussed. Although the occurrence of MAP is worldwide and has been documented in several African countries, the epidemiology and socioeconomic impacts remain undetermined and limited research information is available from the continent. At present, there are still significant knowledge gaps in all these areas as far as Africa is concerned. Due to the limited research on paratuberculosis in Africa, in spite of growing global concerns, it may rightfully be considered a neglected tropical disease with a potentially zoonotic role.
Population Genetics of Phlebotomus papatasi from Endemic and Nonendemic Areas for Zoonotic Cutaneous Leishmaniasis in Morocco, as Revealed by Cytochrome Oxidase Gene Subunit I Sequencing
Authors: Guernaoui Souad , Hamarsheh Omar , Garcia Deborah , Fontenille Didier , Sereno Denis
Published: July 2020

Zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania major Yakimoff & Shokhor and transmitted by Phlebotomus papatasi (Scopoli) is a public health concern in Morocco. The disease is endemic mainly in pre-Saharan regions on the southern slope of the High Atlas Mountains. The northern slope of the High Atlas Mountains and the arid plains of central Morocco remain non-endemic and are currently considered high risk for ZCL. Here we investigate and compare the population genetic structure of P. papatasi populations sampled in various habitats in historical foci and non-endemic ZCL areas. A fragment of the mtDNA cytochrome oxidase I (COI) gene was amplified and sequenced in 59 individuals from 10 P. papatasi populations. Haplotype diversity was probed, a median-joining network was generated (FST) and molecular variance (AMOVA) were analyzed. Overall, we identified 28 haplotypes with 32 distinct segregating sites, of which seven are parsimony informative. The rate of private haplotypes was high; 20 haplotypes (71.4%) are private ones and exclusive to a single population. The phylogenetic tree and the network reconstructed highlight a genetic structuration of these populations in two well defined groups: Ouarzazate (or endemic areas) and Non-Ouarzazate (or nonendemic areas). These groups are separated by the High Atlas Mountains. Overall, our study highlights differences in terms of population genetics between ZCL endemic and non-endemic areas. To what extent such differences would impact the transmission of L. major by natural P. papatasi population remains to be investigated.