bluuebunny/crossref_metadata_2025 huggingface dataset:
Deep Sequencing and Analysis of Transcriptomes of Pinus koraiensis Sieb. & Zucc.
Authors: Shi Shaolin , Yan Siyu , Zhao Chao , Zhang Peng , Yang Ling , Wang Chao , Shen Hailong
Published: March 2020

The objective of this research was to study the differences in endogenous hormone levels and the genes related to reproductive development in Chinese pinenut (Pinus koraiensis) trees of different ages. The apical buds of P. koraiensis were collected from 2-, 5-, 10-, 15-, and 30-year-old plants and also from grafted plants. There were three replicates from each group used for transcriptome sequencing. After assembly and annotation, we identified the differentially expressed genes (DEGs) and performed enrichment analysis, pathway analysis, and expression analysis of the DEGs in each sample. The results showed that unigenes related to reproductive development, such as c64070.graph_c0 and c68641.graph_c0, were expressed at relatively low levels at young ages, and that the relative expression gradually increased with increasing plant age. In addition the highest expression levels were reached around 10 and 15 years of age, after which they gradually decreased. Moreover, some unigenes, such as c61855.graph_c0, were annotated as abscisic acid hydroxylase genes, and the expression of c61855.graph_c0 gradually declined with increasing age in P. koraiensis.
Steam Explosion Pretreatment of Sludge for Pharmaceutical Removal and Heavy Metal Release to Improve Biodegradability and Biogas Production
Authors: Lotfi Aski Abolfazl , Borghei Alimohammad , Zenouzi Ali , Ashrafi Nariman , Taherzadeh Mohammad J.
Published: March 2020

Steam explosion pretreatment was developed and evaluated to remove pharmaceuticals and heavy metals from wastewater sludge and to improve its biodegradability and methane yield. Effects of pressure (5–15 bar) and duration (1–15 min) during the pretreatment were examined, and the pretreatment efficiency was evaluated based on the solubilization degree, the capillary suction time (CST) test and anaerobic digestion. The removal efficiency of ibuprofen, acetaminophen, and amoxicillin was 65%, 69%, and 66% and 70%, 66%, and 70% in primary sludge (PS) and waste-activated sludge (WAS), respectively. The highest percent release efficiency of heavy metals, i.e., lead, cadmium, and silver, for PS and WAS was 78%, 70%, and 79% and 79%, 80%, and 75%, respectively. The highest methane yield was obtained after pretreatment at 10 bar for 15 min and at 15 bar for 10 min, with respective yields of 380 and 358 mL CH4/g volatile solids (VS) for the PS and 315 and 334 mL CH4/g VS for the WAS. The results of methane production indicated that the decreased concentrations of pharmaceuticals and heavy metals resulted in increased biodegradability of PS and WAS.
Influence of Cooling Methods on the Behavior of Reactive Powder Concrete Exposed to Fire Flame Effect
Authors: Awad Hadeel K.
Published: March 2020

The construction of highly safe and durable buildings that can bear accident damage risks including fire, earthquake, impact, and more, can be considered to be the most important goal in civil engineering technology. An experimental investigation was prepared to study the influence of adding various percentages 0%, 1.0%, and 1.5% of micro steel fiber volume fraction (Vf) to reactive powder concrete (RPC)—whose properties are compressive strength, splitting tensile strength, flexural strength, and absorbed energy—after the exposure to fire flame of various burning temperatures 300, 400, and 500 °C using gradual-, foam-, and sudden-cooling methods. The outcomes of this research proved that the maximum reduction in mechanical properties is detected in case of 0% addition at burning temperature of 500 °C using sudden cooling to be 63.90%, 55.77% and 53.8% for compressive, splitting tensile, and flexural strength, respectively, while using 1.5% produced a modification in compressive strength, splitting tensile strength, and flexural strength to 6.67%, 4.15%, and 7.00% respectively, and 7.10 kN·mm for the absorbed energy for gradual cooling at 300 °C. From the results, the adopted cooling methods can be ordered according to their negative influence by sudden, foam, and gradual, while the optimum percentage of (Vf) is 1.5% when burning at 300 °C for all methods of cooling. 1.0% is considered the optimum percentage for all burning temperatures that exceed 400 °C using sudden-cooling method.
Characterization of Flavonoid Compounds in Common Swedish Berry Species
Authors: Liu Jiyun , Hefni Mohammed E. , Witthöft Cornelia M.
Published: March 2020

Berries are considered an ideal source of polyphenols, especially from the flavonoid group. In this study, we examined the flavonoid content in 16 varieties of Swedish lingonberry, raspberry, blueberry, and strawberry. Nineteen flavonoids were simultaneously quantified using external standards. An additional 29 flavonoids were tentatively identified using MS as no standards were available. Quantification was done using HPLC-UV after optimization of chromatographic and extraction procedures. The method showed high linearity within the range of 2–100 μg/mL (correlation co-efficient >0.999), intra- and inter-day precision of 1.7–7.3% and average recovery above 84% for all compounds. Blueberries and lingonberries were found to contain higher contents of flavonoids (1100 mg/100 g dry weight) than raspberries and strawberries (500 mg/100 g dry weight). Anthocyanins were the dominant flavonoids in all berries. The tentatively characterized compounds contribute 18%, 29%, 61%, and 67% of the total flavonoid content in strawberries, lingonberries, raspberries, and blueberries, respectively. Overall, Swedish berries were shown to be good sources of polyphenols.
Textural and Sensory Features Changes of Gluten Free Muffins Based on Rice Sourdough Fermented with Lactobacillus spicheri DSM 15429
Authors: Chiş Maria Simona , Păucean Adriana , Man Simona Maria , Mureşan Vlad , Socaci Sonia Ancuţa , Pop Anamaria , Stan Laura , Rusu Bogdan , Muste Sevastiţa
Published: March 2020

Gluten free products available on the market have a low textural quality associated with high crumbly structure, low-flavor, aroma, poor mouthfeel, less appearance, in comparison with the conventional final baked products. The aim of this study was to assess the influence of rice sourdough fermented with Lactobacillus spicheri DSM 15429 strain on textural, volatile profile, and sensorial properties of gluten free muffins in order to obtain baked goods with improved quality characteristics. Lactobacillus spicheri is a novel strain isolated from industrial rice sourdough but unexploited for bakery products manufacturing. The results showed that Lactobacillus spicheri DSM 15429 was able to growth in the rice flour influencing the texture and the volatile profile of gluten free muffins as well as their sensory characteristics. Both, textural parameters and volatiles recorded significant differences comparing to muffins obtained with a spontaneously fermented rice sourdough. Hardness and cohesiveness decreased while springiness and resilience of gluten free muffins improved their values. The volatile profile of gluten free muffins was significantly improved by utilization of the rice sourdough fermented with Lactobacilus spicheri DSM 15429. 3-methylbutanal, 2-methylbutanal, acetophenone and limonene were the main volatile derivatives responsible for aroma and odor scores of sensory analysis.
Shaping Planetary Nebulae with Jets and the Grazing Envelope Evolution
Authors: Soker Noam
Published: March 2020

I argue that the high percentage of planetary nebulae (PNe) that are shaped by jets show that main sequence stars in binary systems can accrete mass at a high rate from an accretion disk and launch jets. Not only does this allow jets to shape PNe, but this also points to the importance of jets in other types of binary systems and in other processes. These processes include the grazing envelope evolution (GEE), the common envelope evolution (CEE), and the efficient conversion of kinetic energy to radiation in outflows. Additionally, the jets point to the possibility that many systems launch jets as they enter the CEE, possibly through a GEE phase. The other binary systems in which jets might play significant roles include intermediate-luminosity optical transients (ILOTs), supernova impostors (including pre-explosion outbursts), post-CEE binary systems, post-GEE binary systems, and progenitors of neutron star binary systems and black hole binary systems. One of the immediate consequences is that the outflow of these systems is highly-non-spherical, including bipolar lobes, jets, and rings.
Flipping Our Scripts about Undocumented Immigration
Authors: Pollock Mica
Published: March 2020

This critical family history explores a common script about undocumented immigration: that undocumented immigrants unfairly have refused to “stand in line” for official, sanctioned immigration and instead have broken rules that the rest of “our” families have followed. Noting a hole in her knowledge base, the author put herself on a steep learning curve to “clean her lenses”—to learn more information about opportunities past and present, so she could see and discuss the issue more clearly. The author sought new and forgotten information about immigration history, new information about her own family, and details about actual immigration policy. She wrote this piece to share a few script-flipping realizations, in case they can shortcut this journey for others.
Fetal Hypoxia Impacts on Proliferation and Differentiation of Sca-1+ Cardiac Progenitor Cells and Maturation of Cardiomyocytes: A Role of MicroRNA-210
Authors: Meng Xianmei , Zhang Peng , Zhang Lubo
Published: March 2020

Hypoxia is one of the most frequent and severe stresses to an organism’s homeostatic mechanisms, and hypoxia during gestation has profound adverse effects on the heart development increasing the occurrence of congenital heart defects (CHDs). Cardiac progenitor cells (CPCs) are responsible for early heart development and the later occurrence of heart disease. However, the mechanism of how hypoxic stress affects CPC fate decisions and contributes to CHDs remains a topic of debate. Here we examined the effect of hypoxic stress on the regulations of CPC fate decisions and the potential mechanism. We found that experimental induction of hypoxic responses compromised CPC function by regulating CPC proliferation and differentiation and restraining cardiomyocyte maturation. In addition, echocardiography indicated that fetal hypoxia reduced interventricular septum thickness at diastole and the ejection time, but increased the heart rate, in mouse young adult offspring with a gender-related difference. Further study revealed that hypoxia upregulated microRNA-210 expression in Sca-1+ CPCs and impeded the cell differentiation. Blockage of microRNA-210 with LNA-anti-microRNA-210 significantly promoted differentiation of Sca-1+ CPCs into cardiomyocytes. Thus, the present findings provide clear evidence that hypoxia alters CPC fate decisions and reveal a novel mechanism of microRNA-210 in the hypoxic effect, raising the possibility of microRNA-210 as a potential therapeutic target for heart disease.
NLGenomeSweeper: A Tool for Genome-Wide NBS-LRR Resistance Gene Identification
Authors: Toda Nicholas , Rustenholz Camille , Baud Agnès , Le Paslier Marie-Christine , Amselem Joelle , Merdinoglu Didier , Faivre-Rampant Patricia
Published: March 2020

Although there are a number of bioinformatic tools to identify plant nucleotide-binding leucine-rich repeat (NLR) disease resistance genes based on conserved protein sequences, only a few of these tools have attempted to identify disease resistance genes that have not been annotated in the genome. The overall goal of the NLGenomeSweeper pipeline is to annotate NLR disease resistance genes, including RPW8, in the genome assembly with high specificity and a focus on complete functional genes. This is based on the identification of the complete NB-ARC domain, the most conserved domain of NLR genes, using the BLAST suite. In this way, the tool has a high specificity for complete genes and relatively intact pseudogenes. The tool returns all candidate NLR gene locations as well as InterProScan ORF and domain annotations for manual curation of the gene structure.
Bottom Sediment Characteristics of a Tropical Lake: Lake Tana, Ethiopia
Authors: Kebedew Mebrahtom G. , Tilahun Seifu A. , Zimale Fasikaw A. , Steenhuis Tammo S.
Published: March 2020

Sediment concentration of rivers in developing countries has been increasing greatly over the last 50 years due to the conversion of forest to continuously cultivated land with the increasing population. Few studies have addressed its effect on sedimentation and water quality of the lakes by analyzing bottom sediment characteristics. In this study, the objective was to investigate the spatial distribution (and their interrelationships) of the bottom sediment characteristics in the largest lake in Ethiopia, Lake Tana where water hyacinths have been spreading rapidly during the last decade. Sediment samples were collected from the lake bottom at 60 locations and analyzed for texture, organic matter, total nitrogen, and available phosphorus. Bottom sediment samples had a median of 75% clay, 13% silt, and 9% sand. Clay was greatest in the northwestern part and smallest in the areas near the major rivers entering or exiting the lake. Clay percentage and lake depth were strongly correlated. The mean organic matter content of bottom sediment was 16 g kg−1, total nitrogen 0.8 g kg−1, and Olsen available phosphorus 19 mg kg−1. Phosphorus concentrations peaked where water hyacinths were found in the northeastern part of the lake. This study will serve as a baseline for future water quality and sedimentation changes in Lake Tana. In particular, it might aid in explaining the spread of the water hyacinths.
Using a Family Systems Approach to Treat Tobacco Use among Cancer Patients
Authors: Ruebush Ellen , Mitra Sara , Meyer Colleen , Sisler Laurel , Goldstein Adam O.
Published: March 2020

Tobacco use treatment is an essential component of cancer care. Family members play a significant role in smoking behavior, but more research is needed regarding the development, implementation, and impact of family-based interventions in cancer care. The UNC Tobacco Treatment Program conducted an 18-month pilot study to examine the feasibility of implementing a family systems approach to treat tobacco use among patients at the North Carolina Cancer Hospital and to measure the impact of such an approach on patient abstinence. Implementation included four phases: (1) modifying the electronic health record and monthly report generated from the electronic health record; (2) training Tobacco Treatment Specialists to provide family counseling; (3) integrating family members into patients’ treatment; and (4) conducting six-month follow-up calls. During the course of the study, 42% (N = 221/532) of patients had family members integrated into their tobacco use treatment. Only 21 patients (4%) had family members present but not integrated into the treatment plan. At the six-month follow up time point, the seven-day point-prevalence quit rate for patients with family integration was 28% (N = 56/200), compared to 23% (N = 67/291) (p = 0.105) for patients without family integration. Integration of family members is clearly possible in an academic medical center’s oncology tobacco treatment program. Although pilot results were not statistically significant at 6 months, a potentially higher quit rate suggests a need for expanded research on methods to integrate family members in oncology settings for patients with tobacco-related cancers.
Why Do Women Not Use the Bathroom? Women’s Attitudes and Beliefs on Using Public Restrooms
Authors: Hartigan Siobhan M. , Bonnet Kemberlee , Chisholm Leah , Kowalik Casey , Dmochowski Roger R. , Schlundt David , Reynolds W. Stuart
Published: March 2020

There are a variety of factors and influences, both internal and external, that may impact an individual’s public toileting experience and may ultimately have repercussions for bladder health. This study sought to identify predominant constructs underlying a women’s attitude towards using restrooms at work, at school, and in public in order to develop a conceptual model incorporating these themes. We performed a secondary analysis of a cross-sectional, survey-based study that included open-ended questions about limitations to restroom use using a mixed-methods approach. Qualitative data coding and analysis was performed on 12,583 quotes and, using an iterative inductive-deductive approach, was used to construct the conceptual framework. Our conceptual framework reveals a complicated interplay of personal contexts, situational influences, and behavioral strategies used by women to manage their bladder and bowel habits away from home. These findings can inform future research and public policy related to bladder health awareness related to toilet access in the workplace and in public.
Legal Steps to Secure the Tobacco Supply Chain: A Case Study of Poland
Authors: Balwicki Łukasz , Stoklosa Michal , Balwicka-Szczyrba Małgorzata , Drope Jeffrey
Published: March 2020

The threat of tobacco tax evasion and avoidance is the most commonly mentioned argument against tax hikes. Increasingly, the focus of legislators is on leaks in the tobacco crop supply chain, in which raw or cured tobacco that was never taxed finds its way to smokers. To study the process undertaken by Poland to secure the tobacco supply chain, we analyzed the 2013–2018 legislation around tobacco supply and interviewed a key stakeholder in the Government of Poland. We found that farmers and intermediary entities can trade tobacco only if registered with the government. Farmers are required to report the size of their fields and the weight of their crops to the state authorities. Each purchase within the supply chain is also reported by both the seller and the buyer for cross-validation. This has prevented manipulation within the system, while the mere threat of heavy fines related to an excise tax law violation and/or the administrative burden associated with becoming an excise tax payer (had the violation been prosecuted) has significantly contributed to securing the tobacco supply chain. The experience of Poland demonstrates that securing the tobacco supply chain is complicated but also a tractable problem. This case can be widely applicable to other countries.
Do the Walkability and Urban Leisure Amenities of Neighborhoods Affect the Body Mass Index of Individuals? Based on a Case Study in Seoul, South Korea
Authors: Choi Yunwon , Yoon Heeyeun
Published: March 2020

This study investigates the impact of neighborhood-built environments on obesity in interrelationship with socioeconomic status (SES)—controlling for dietary patterns and physical activities of residents—using structural equation modeling (SEM). A total of 577 samples who are between 19 and 64 years old and reside in Seoul are extracted from Korea National Health and Nutrition Examination Survey (KNHNES), 2015. Neighborhood environments are represented as the two latent constructs—walkability and leisure amenities—composited with indicators such as density of intersections, density of mixed-use area, and the area of open spaces and are aggregated by jurisdictional unit in Seoul. We found that greater walkability in a neighborhood explained a lower body mass index (BMI) among residents, whereas more urban leisure amenities in a neighborhood explained a higher BMI. The finding suggests that a walking-friendly environment is more effective than active recreational amenities in inducing people to engage in daily physical activities to the level that reduces obesity rate. SES exerted a negative impact on BMI of a greater magnitude than the impact of either of the environmental living conditions, reinforcing the importance of general wealth and education level in leading to a healthy lifestyle. Our research contributes to growing evidence of a relationship between obesity and the built environment in the context of Asian countries where the prevalence of obesity is becoming a serious issue and requires immediate attention.
Analysis of the Components of a Cognitive-Behavioral Intervention for the prevention of Depression Administered via Conference Call to Nonprofessional Caregivers: A Randomized Controlled Trial
Authors: Vázquez Fernando L. , López Lara , Torres Ángela J. , Otero Patricia , Blanco Vanessa , Díaz Olga , Páramo Mario
Published: March 2020

Effective and accessible interventions for indicated prevention of depression are necessary and lacking, especially for informal caregivers. Although telephone-based interventions could increase the accessibility for caregivers, randomized controlled trials are scarce, with no examination of prevention to date. Moreover, the efficacy of specific therapeutic components in preventive cognitive-behavioral programs is unknown. The main objective of this study was to evaluate the efficacy of a telephone-administered psychological preventive intervention in informal caregivers with high depressive symptoms. A total of 219 caregivers were randomized to a cognitive-behavioral conference call intervention (CBCC, n = 69), a behavioral-activation conference call intervention (BACC, n = 70), or a usual care control group (CG, n = 80). Both interventions consisted of five 90-minute group sessions. At the post-intervention, incidence of depression was lower in CBCC and BACC compared to CG (1.5% and 1.4% vs. 8.8%). Relative risk was 0.17 for the CBCC and 0.16 for the BACC, and the number-needed-to-treat was 14 in both groups. Depressive symptoms were significantly lower in BACC and BACC groups compared to CG (d = 1.16 and 1.29), with no significant differences between CBCC and BACC groups. The conference call intervention was effective in preventing depression and the behavioral-activation component (BACC) was comparable to the CBCC intervention.
Shifting the Care of Type 2 Diabetes Mellitus from Hospital to Primary Health Care Institutions through an Educational Intervention for Health Care Professionals: An Example from Rural China
Authors: Chen Shaofan , Qian Dongfu , Burström Bo
Published: March 2020

This study assessed the impact of an educational intervention on the knowledge, attitudes, and practice regarding Type 2 Diabetes Mellitus (T2DM) of Primary Health Care (PHC) professionals, as well as on the types of T2DM care services which they were able to provide. The intervention was carried out in collaboration with county hospitals. The study was conducted from 2015 to 2016 among 241 health care professionals in 18 township health centers and 55 village clinics in three counties in Jiangsu Province, randomly divided into an intervention group and a control group. Participants in the intervention group received professional skills training sessions and team communication and were involved in regular meetings. The control group followed the routine work plan. At one-year follow up, the diabetes knowledge score, practice score, and attitudes score were significantly higher in the intervention group than in the control group. A significantly higher proportion of health care professionals in the intervention group was able to provide services compared with the control group, for all types of services, except T2DM emergency treatment. The intervention among health care professionals in PHC had a positive impact on their professional diabetes skills, knowledge, attitudes, practices, and types of services they were able to provide, at one-year follow-up.
piRNAs in Gastric Cancer: A New Approach Towards Translational Research
Authors: Fonseca Cabral Gleyce , Azevedo dos Santos Pinheiro Jhully , Vidal Amanda Ferreira , Santos Sidney , Ribeiro-dos-Santos Ândrea
Published: March 2020

Background: Gastric cancer is currently the third leading cause of cancer-related deaths worldwide, usually diagnosed at late stages. The development of new biomarkers to improve its prevention and patient management is critical for disease control. piRNAs are small regulatory RNAs important for gene silencing mechanisms, mainly associated with the silencing of transposable elements. piRNA pathways may also be involved in gene regulation and the deregulation of piRNAs may be an important factor in carcinogenic processes. Thus, several studies suggest piRNAs as potential cancer biomarkers. Translational studies suggest that piRNAs may regulate key genes and pathways associated with gastric cancer progression, though there is no functional annotation in piRNA databases. The impacts of genetic variants in piRNA genes and their influence in gastric cancer development remains elusive, highlighting the gap in piRNA regulatory mechanisms knowledge. Here, we discuss the current state of understanding of piRNA-mediated regulation and piRNA functions and suggest that genetic alterations in piRNA genes may affect their functionality, thus, it may be associated with gastric carcinogenesis. Conclusions: In the era of precision medicine, investigations about genetic and epigenetic mechanisms are essential to further comprehend gastric carcinogenesis and the role of piRNAs as potential biomarkers for translational research.
Complement Component C3 Participates in Early Stages of Niemann–Pick C Mouse Liver Damage
Authors: Klein Andrés D. , González de la Vega Javier , Zanlungo Silvana
Published: March 2020

Niemann–Pick type C (NPC), a lysosomal storage disorder, is mainly caused by mutations in the NPC1 gene. Niemann–Pick type C patients and mice show intracellular cholesterol accumulation leading to hepatic failure with increased inflammatory response. The complement cascade, which belongs to the innate immunity response, recognizes danger signals from injured tissues. We aimed to determine whether there is activation of the complement system in the liver of the NPC mouse and to assess the relationship between C3 activation, a final component of the pathway, and NPC liver pathology. Niemann–Pick type C mice showed high levels of C3 staining in the liver which unexpectedly decreased with aging. Using an inducible NPC1 hepatocyte rescue mouse model, we restored NPC1 expression for a short time in young mice. We found C3 positive cells only in non-rescued cells, suggesting that C3 activation in NPC cells is reversible. Then, we studied the effect of C3 ablation on NPC liver damage at two postnatal time points, P56 and P72. Deletion of C3 reduced the presence of hepatic CD68-positive cells at postnatal day 56 and prevented the increase of transaminase levels in the blood of NPC mice. These positive effects were abrogated at P72, indicating that the complement cascade participates only during the early stages of liver damage in NPC mice, and that its inhibition may serve as a new potential therapeutic strategy for the disease.
Modification of the Mammalian Endomembrane System in Healthy and Diseased Cells
Authors: Simpson Jeremy C.
Published: March 2020

One remarkable characteristic of eukaryotic cells is the complexity of their membrane systems [...]
Distal Unfolding of Ferricytochrome c Induced by the F82K Mutation
Authors: Lalli Daniela , Rosa Camilla , Allegrozzi Marco , Turano Paola
Published: March 2020

It is well known that axial coordination of heme iron in mitochondrial cytochrome c has redox-dependent stability. The Met80 heme iron axial ligand in the ferric form of the protein is relatively labile and can be easily replaced by alternative amino acid side chains under non-native conditions induced by alkaline pH, high temperature, or denaturing agents. Here, we showed a redox-dependent destabilization induced in human cytochrome c by substituting Phe82—conserved amino acid and a key actor in cytochrome c intermolecular interactions—with a Lys residue. Introducing a positive charge at position 82 did not significantly affect the structure of ferrous cytochrome c but caused localized unfolding of the distal site in the ferric state. As revealed by 1H NMR fingerprint, the ferric form of the F82K variant had axial coordination resembling the renowned alkaline species, where the detachment of the native Met80 ligand favored the formation of multiple conformations involving distal Lys residues binding to iron, but with more limited overall structural destabilization.
High-Protein Diet Induces Hyperuricemia in a New Animal Model for Studying Human Gout
Authors: Hong Fan , Zheng Aijuan , Xu Pengfei , Wang Jialin , Xue Tingting , Dai Shu , Pan Shijia , Guo Yuan , Xie Xinlu , Li Letong , Qiao Xiaoxiao , Liu Guohua , Zhai Yonggong
Published: March 2020

Hyperuricemia is a central risk factor for gout and increases the risk for other chronic diseases, including cardiometabolic disease, kidney disease, and hypertension. Overproduction of urate is one of the main reasons for hyperuricemia, and dietary factors including seafoods, meats, and drinking are contributed to the development of it. However, the lack of a suitable animal model for urate metabolism is one of the main reasons for the delay and limitations of hyperuricemia research. Combining evolutionary biological studies and clinical studies, we conclude that chicken is a preferred animal model for hyperuricemia. Thus, we provided chickens a high-protein diet (HPD) to evaluate the changes in the serum urate levels in chickens. In our study, the HPD increased the serum urate level and maintained it at a long-term high level in chickens. Long-term high serum urate levels induced an abnormal chicken claw morphology and the precipitation of monosodium urate (MSU) in joint synovial fluid. In addition, a long-term HPD also decreased the glomerular filtration rate and induced mild renal injury. Most importantly, allopurinol and probenecid displayed the positive effects in decreasing serum urate and then attenuated hyperuricemia in chicken model. These findings provide a novel model for hyperuricemia and a new opportunity to further investigate the effects of long-term hyperuricemia on other metabolic diseases.
Astaxanthin n-Octanoic Acid Diester Ameliorates Insulin Resistance and Modulates Gut Microbiota in High-Fat and High-Sucrose Diet-Fed Mice
Authors: Gao Yuan , Yang Lu , Chin Yaoxian , Liu Fang , Li Robert W. , Yuan Shihan , Xue Changhu , Xu Jie , Tang Qingjuan
Published: March 2020

Astaxanthin n-octanoic acid diester (AOD) is a type of astaxanthin connecting medium-chain fatty acids with a more stable structure. In this study, we examined the role of AOD in ameliorating insulin resistance (IR) induced by a high-fat and high-sucrose diet (HFD) as well as its effect on modulating gut microbiota in mice, with free astaxanthin (AST) as a comparison. Four groups of male C57BL/6J mice (6 weeks old; n = 10 per group) were fed with a normal control diet (NC), HFD orally administered with AOD, AST (50 mg/kg body weight), or vehicle for 8 weeks. AOD improved glucose tolerance, IR, systematic and intestinal inflammation, and intestinal integrity better than AST. Further, both AOD and AST modulated gut microbiota. A significantly higher abundance of Bacteroides and Coprococcus was found in AOD than in AST, and the predicted pathway of carbohydrate metabolism was significantly impacted by AOD. Overall, AOD may play a role in alleviating IR and inflammation with the modulating effect on microbiota in HFD-fed mice. Our findings could facilitate the development of AOD as a bioactive nutraceutical and more stable alternative to AST.
Gomisin N from Schisandra chinensis Ameliorates Lipid Accumulation and Induces a Brown Fat-Like Phenotype through AMP-Activated Protein Kinase in 3T3-L1 Adipocytes
Authors: Lee Kippeum , Lee Yeon-Joo , Kim Kui-Jin , Chei Sungwoo , Jin Heegu , Oh Hyun-Ji , Lee Boo-Yong
Published: March 2020

Obesity results from an imbalance between energy intake and energy expenditure, in which excess fat is stored as triglycerides (TGs) in white adipocytes. Recent studies have explored the anti-obesity effects of certain edible phytochemicals, which suppress TG accumulation and stimulate a brown adipocyte-like phenotype in white adipocytes. Gomisin N (GN) is an important bioactive component of Schisandra chinensis, a woody plant endemic to Asia. GN has antioxidant, anti-inflammatory and hepatoprotective effects in vivo and in vitro. However, the anti-obesity effects of GN in lipid metabolism and adipocyte browning have not yet been investigated. In the present study, we aimed to determine whether GN suppresses lipid accumulation and regulates energy metabolism, potentially via AMP-activated protein kinase (AMPK), in 3T3-L1 adipocytes. Our findings demonstrate that GN inhibited adipogenesis and lipogenesis in adipocyte differentiation. Also, GN not only increased the expression of thermogenic factors, including uncoupling protein 1 (UCP1), but also enhanced fatty acid oxidation (FAO) in 3T3-L1 cells. Therefore, GN may have a therapeutic benefit as a promising natural agent to combat obesity.
Radon Biomonitoring and microRNA in Lung Cancer
Authors: Bersimbaev Rakhmet , Pulliero Alessandra , Bulgakova Olga , Kussainova Assiya , Aripova Akmara , Izzotti Alberto
Published: March 2020

Radon is the number one cause of lung cancer in non-smokers. microRNA expression in human bronchial epithelium cells is altered by radon, with particular reference to upregulation of miR-16, miR-15, miR-23, miR-19, miR-125, and downregulation of let-7, miR-194, miR-373, miR-124, miR-146, miR-369, and miR-652. These alterations alter cell cycle, oxidative stress, inflammation, oncogene suppression, and malignant transformation. Also DNA methylation is altered as a consequence of miR-29 modification induced by radon. Indeed miR-29 targets DNA methyltransferases causing inhibition of CpG sites methylation. Massive microRNA dysregulation occurs in the lung due to radon expose and is functionally related with the resulting lung damage. However, in humans this massive lung microRNA alterations only barely reflect onto blood microRNAs. Indeed, blood miR-19 was not found altered in radon-exposed subjects. Thus, microRNAs are massively dysregulated in experimental models of radon lung carcinogenesis. In humans these events are initially adaptive being aimed at inhibiting neoplastic transformation. Only in case of long-term exposure to radon, microRNA alterations lead towards cancer development. Accordingly, it is difficult in human to establish a microRNA signature reflecting radon exposure. Additional studies are required to understand the role of microRNAs in pathogenesis of radon-induced lung cancer.
Development of a Cartilage Oligomeric Matrix Protein Neo-Epitope Assay for the Detection of Intra-Thecal Tendon Disease
Authors: Smith Roger , Önnerfjord Patrik , Holmgren Kristin , di Grado Shacko , Dudhia Jayesh
Published: March 2020

The diagnosis of tendon injury relies on clinical signs and diagnostic imaging but imaging is subjective and does not always correlate with clinical signs. A molecular marker would potentially offer a sensitive and specific diagnostic tool that could also provide objective assessment of healing for the comparison of different treatments. Cartilage Oligomeric Matrix Protein (COMP) has been used as a molecular marker for osteoarthritis in humans and horses but assays for the protein in tendon sheath synovial fluids have shown overlap between horses affected by tendinopathy and controls. We hypothesized that quantifying a COMP neoepitope would be more discriminatory of injury. COMP fragments were purified from synovial fluids of horses with intra-thecal tendon injuries and media from equine tendon explants, and mass spectrometry of a consistent and abundant fragment revealed a ~100 kDa COMP fragment with a new N-terminus at the 78th amino-acid (NH2-TPRVSVRP) located just outside the junctional region of the protein. A competitive inhibition ELISA based on a polyclonal antibody raised to this sequence yielded more than a 10-fold rise in the mean neoepitope levels for tendinopathy cases compared to controls (5.3 ± 1.3 µg/mL (n = 7) versus 58.8 ± 64.3 µg/mL (n = 13); p = 0.002). However, there was some cross-reactivity of the neoepitope polyclonal antiserum with intact COMP, which could be blocked by a peptide spanning the neoepitope. The modified assay demonstrated a lower concentration but a significant > 500-fold average rise with tendon injury (2.5 ± 2.2 ng/mL (n = 6) versus 1029.8 ± 2188.8 ng/ml (n = 14); p = 0.013). This neo-epitope assay therefore offers a potentially useful marker for clinical use.
Interactions of Whey Proteins with Metal Ions
Authors: Rodzik Agnieszka , Pomastowski Paweł , Sagandykova Gulyaim N. , Buszewski Bogusław
Published: March 2020

Whey proteins tend to interact with metal ions, which have implications in different fields related to human life quality. There are two impacts of such interactions: they can provide opportunities for applications in food and nutraceuticals, but may lead to analytical challenges related to their study and outcomes for food processing, storage, and food interactions. Moreover, interactions of whey proteins with metal ions are complicated, requiring deep understanding, leading to consequences, such as metalloproteins, metallocomplexes, nanoparticles, or aggregates, creating a biologically active system. To understand the phenomena of metal–protein interactions, it is important to develop analytical approaches combined with studies of changes in the biological activity and to analyze the impact of such interactions on different fields. The aim of this review was to discuss chemistry of β-lactoglobulin, α-lactalbumin, and lactotransferrin, their interactions with different metal ions, analytical techniques used to study them and the implications for food and nutraceuticals.
Evaluation of Content Dissemination Strategies in Urban Vehicular Networks
Authors: Pessoa Gonçalo , Guardalben Lucas , Luís Miguel , Senna Carlos , Sargento Susana
Published: March 2020

The main drivers for the continuous development of Vehicular ad-hoc Networks (VANETs) are safety applications and services. However, in recent years, new interests have emerged regarding the introduction of new applications and services for non-urgent content (e.g., videos, ads, sensing and touristic information) dissemination. However, there is a lack of real studies considering content dissemination strategies to understand when and to whom the content should be disseminated using real vehicular traces gathered from real vehicular networks. This work presents a realistic study of strategies for dissemination of non-urgent content with the main goal of improving content delivery as well as minimizing network congestion and resource usage. First, we perform an exhaustive network characterization. Then, several content strategies are specified and evaluated in different scenarios (city center and parking lot). All the obtained results show that there are two content distribution strategies that clearly set themselves apart due to their superior performance: Local Rarest Bundle First and Local Rarest Generation First.
System Potential Estimation with Regard to Digitalization: Main Ideas and Estimation Example
Authors: Geyda Alexander , Lysenko Igor
Published: March 2020

The article outlines the main concept and examples of mathematical models needed to estimate system potential and digitalization performance indicators. Such an estimation differs in that it is performed with predictive mathematical models. The purpose of such an estimation is to enable a set of problems of system design and functional design of information technologies to be solved as mathematical problems, predictively and analytically. The hypothesis of the research is that the quality of system functioning in changing conditions can be evaluated analytically, based on predictive mathematical models. We suggested a property of the system potential (or system capability) that describes the effects of the compliance of changing system functioning with changing conditions analytically and predictively. Thus, it describes the performance of the use of information operations to realize functioning in changing conditions. The example includes system’s environment graph-theoretic models and system’s models regarding IT use to react to changing environments. As a result of the suggested models and methods, the quantitative estimation of system potential regarding information technology use becomes possible depending on the parameters and variables of the problems to be solved. Use cases of problems decision with the use of such indicators include choosing optimal information technology implementation and the synthesis of information operation characteristics.
Carotid and Femoral Atherosclerotic Plaques in Asymptomatic and Non-Treated Subjects: Cardiovascular Risk Factors, 10-Years Risk Scores, and Lipid Ratios’ Capability to Detect Plaque Presence, Burden, Fibro-Lipid Composition and Geometry
Authors: Marin Mariana , Bia Daniel , Zócalo Yanina
Published: March 2020

Carotid and/or femoral atherosclerotic plaques (AP) assessment through imaging studies is an interesting strategy for improving individual cardiovascular risk (CVR) stratification and cardiovascular disease (CVD) and/or events prediction. There is no consensus on who would benefit from image screening aimed at determining AP presence, burden, and characteristics. Aims: (1) to identify, in asymptomatic and non-treated subjects, demographic factors, anthropometric characteristics and cardiovascular risk factors (CRFs), individually or grouped (e.g., CVR equations, pro-atherogenic lipid ratios) associated with carotid and femoral AP presence, burden, geometry, and fibro-lipid content; (2) to identify cut-off values to be used when considering the variables as indicators of increased probability of AP presence, elevated atherosclerotic burden, and/or lipid content, in a selection scheme for subsequent image screening. Methods: CRFs exposure and clinical data were obtained (n = 581; n = 144 with AP; 47% females). Arterial (e.g., ultrasonography) and hemodynamic (central [cBP] and peripheral blood pressure; oscillometry/applanation tonometry) data were obtained. Carotid and femoral AP presence, burden (e.g., AP number, involved territories), geometric (area, width, height) and fibro-lipid content (semi-automatic, virtual histology analysis, grayscale analysis and color mapping) were assessed. Lipid profile was obtained. Lipid ratios (Total cholesterol/HDL-cholesterol, LDL-cholesterol/HDL-cholesterol, LogTryglicerides(TG)/HDL-cholesterol) and eight 10-years [y.]/CVR scores were quantified (e.g., Framingham Risk Scores [FRS] for CVD). Results: Age, 10-y./CVR and cBP showed the highest levels of association with AP presence and burden. Individually, classical CRFs and lipid ratios showed almost no association with AP presence. 10-y./CVR levels, age and cBP enabled detecting AP with large surfaces (˃p75th). Lipid ratios showed the largest association with AP fibro-lipid content. Ultrasound evaluation could be considered in asymptomatic and non-treated subjects aiming at population screening of AP (e.g., ˃ 45 y.; 10-y./FRS-CVD ˃ 5–8%); identifying subjects with high atherosclerotic burden (e.g., ˃50 y., 10-y./FRS-CVD ˃ 13–15%) and/or with plaques with high lipid content (e.g., LogTG/HDL ˃ 0.135).
Performance Assessment of ERA5 Wave Data in a Swell Dominated Region
Authors: Bruno Maria Francesca , Molfetta Matteo Gianluca , Totaro Vincenzo , Mossa Michele
Published: March 2020

The present paper deals with a performance assessment of the ERA5 wave dataset in an ocean basin where local wind waves superimpose on swell waves. The evaluation framework relies on observed wave data collected during a coastal experimental campaign carried out offshore of the southern Oman coast in the Western Arabian Sea. The applied procedure requires a detailed investigation on the observed waves, and aims at classifying wave regimes: observed wave spectra have been split using a 2D partition scheme and wave characteristics have been evaluated for each wave component. Once the wave climate was defined, a detailed wave model assessment was performed. The results revealed that during the analyzed time span the ERA5 wave model overestimates the swell wave heights, whereas the wind waves’ height prediction is highly influenced by the wave developing conditions. The collected field dataset is also useful for a discussion on spectral wave characteristics during monsoon and post-monsoon season in the examined region; the recorded wave data do not suffice yet to adequately describe wave fields generated by the interaction of monsoon and local winds.
Analysis of Piled Concrete Foundation for a 3-MW Class Offshore Wind Turbine along the Southwest Coast in Korea
Authors: Kim Hyun-Gi , Kim Bum-Joon , Lee Kwang-Ho
Published: March 2020

Concrete foundations have received attention as offshore wind turbine support structures because of their various advantages. However, because of the lack of information on structural analysis and the design method of complex marine environmental loads, concrete foundations cannot be applied on actual sites. Therefore, the structure behavior mechanism and concrete reinforcement design need to be evaluated based on soil-structure interactions. Herein, an efficient method for analysis of piled concrete foundations (PCFs) is presented, and the stability of PCF structures is evaluated under environmental conditions of the coast in Korea for a 3-MW wind turbine. Three analytical parameters for PCF models were defined to consider soil-structure interaction. The results of each model were compared with the displacement, stresses, and natural frequencies. Using the analysis results, a prestressing reinforcement design for concrete foundations was proposed. Quasi-static analysis showed that maximum displacement was sufficiently small and the maximum stresses did not exceed the allowable stresses. PCF showed excellent dynamic performance and structural stability. In addition, stiffness of the soil spring model influenced the natural frequency rather than the stiffness of the pile type. Detailed analysis of the connections between piles and concrete need to be studied in the future.
Density and Abundance of Delphinus delphis in Waters South of Samos Island, Greece (Eastern Mediterranean Sea)
Authors: Pietroluongo Guido , Cipriano Giulia , Ashok Karthik , Antichi Simone , Carlier Heloise , Miliou Anastasia , Maglietta Rosalia , Fanizza Carmelo , Carlucci Roberto
Published: March 2020

The Mediterranean subpopulation of short-beaked common dolphin Delphinus delphis is ranked as endangered on the IUCN (International Union for Conservation of Nature) Red List because it has sharply declined during the last decades, resulting in sparse and decreasing populations. Monitoring the conservation status of this endangered dolphin species is particularly relevant to fulfil targets under the range of several international agreements. Moreover, estimating the abundance of D. delphis is essential to verify the effectiveness of conservation action to maintain safe population levels in the Mediterranean Sea and to suggest appropriate modifications to limit potential threats. In this regard, a monitoring program of the short-beaked common dolphin in Samos Island (Greece) was carried out from 2016 to 2019, adopting a random line transect sampling method. The overall density and abundance estimates of D. delphis, obtained by applying conventional distance sampling (CDS) on sighting data, were 0.15 individuals/km2 (CV = 13.27%; 95% CI = 0.11–0.19 individuals/km2) and 51 individuals (CV = 13.27%; 95% CI = 40-66 individuals), respectively. Although, a longer time series of sighting data should be collected and a larger area should be investigated to better understand the population trend of D. delphis and its residency pattern, the results contribute to setting up a baseline reference for future assessment of its population in the Eastern Aegean Sea.