bluuebunny/crossref_metadata_2025 huggingface dataset:
Pharma 4.0-Artificially Intelligent Digital Twins for Solidified Nanosuspensions
Authors: Davidopoulou Christina , Ouranidis Andreas
Published: October 2022

Digital twins capacitate the industry 4.0 paradigm by predicting and optimizing the performance of physical assets of interest, mirroring a realistic in-silico representation of their functional behaviour. Although advanced digital twins set forth disrupting opportunities by delineating the in-service product and the related process dynamic performance, they have yet to be adopted by the pharma sector. The latter, currently struggles more than ever before to improve solubility of BCS II i.e., hard-to-dissolve active pharmaceutical ingredients by micronization and subsequent stabilization. Herein we construct and functionally validate the first artificially intelligent digital twin thread, capable of describing the course of manufacturing of such solidified nanosuspensions given a defined lifecycle starting point and predict and optimize the relevant process outcomes. To this end, we referenced experimental data as the sampling source, which we then augmented via pattern recognition utilizing neural network propagations. The zeta-dynamic potential metrics of the nanosuspensions were correlated to the interfacial Gibbs energy, while the density and heat capacity of the material system was calculated via the Saft-γ-Mie statistical fluid theory. The curated data was then fused to physical and empirical laws to choose the appropriate theory and numeric description, respectively, before being polished by tuning the critical parameters to achieve the best fit with reality.
Toward Optimized 89Zr-Immuno-PET: Side-by-Side Comparison of [89Zr]Zr-DFO-, [89Zr]Zr-3,4,3-(LI-1,2-HOPO)- and [89Zr]Zr-DFO*-Cetuximab for Tumor Imaging: Which Chelator Is the Most Suitable?
Authors: Damerow Helen , Cheng Xia , von Kiedrowski Valeska , Schirrmacher Ralf , Wängler Björn , Fricker Gert , Wängler Carmen
Published: October 2022

89Zr represents a highly favorable positron emitter for application in immuno-PET (Positron Emission Tomography) imaging. Clinically, the 89Zr4+ ion is introduced into antibodies by complexation with desferrioxamine B. However, producing complexes of limited kinetic inertness. Therefore, several new chelators for 89Zr introduction have been developed over the last years. Of these, the direct comparison of the most relevant ones for clinical translation, DFO* and 3,4,3-(LI-1,2-HOPO), is still missing. Thus, we directly compared DFO with DFO* and 3,4,3-(LI-1,2-HOPO) immunoconjugates to identify the most suitable agent stable 89Zr-complexation. The chelators were introduced into cetuximab, and an optical analysis method was developed, enabling the efficient quantification of derivatization sites per protein. The cetuximab conjugates were efficiently obtained and radiolabeled with 89Zr at 37 °C within 30 min, giving the [89Zr]Zr-cetuximab derivatives in high radiochemical yields and purities of >99% as well as specific activities of 50 MBq/mg. The immunoreactive fraction of all 89Zr-labeled cetuximab derivatives was determined to be in the range of 86.5–88.1%. In vivo PET imaging and ex vivo biodistribution studies in tumor-bearing animals revealed a comparable and significantly higher kinetic inertness for both [89Zr]Zr-3,4,3-(LI-1,2-HOPO)-cetuximab and [89Zr]Zr-DFO*-cetuximab, compared to [89Zr]Zr-DFO-cetuximab. Of these, [89Zr]Zr-DFO*-cetuximab showed a considerably more favorable pharmacokinetic profile with significantly lower liver and spleen retention than [89Zr]Zr-3,4,3-(LI-1,2-HOPO)-cetuximab. Since [89Zr]Zr-DFO* demonstrates a very high kinetic inertness, paired with a highly favorable pharmacokinetic profile of the resulting antibody conjugate, DFO* currently represents the most suitable chelator candidate for stable 89Zr-radiolabeling of antibodies and clinical translation.
Hyaluronic Acid-Conjugated PLGA Nanoparticles Alleviate Ulcerative Colitis via CD44-Mediated Dual Targeting to Inflamed Colitis Tissue and Macrophages
Authors: Hlaing Shwe Phyu , Cao Jiafu , Lee Juho , Kim Jihyun , Saparbayeva Aruzhan , Kwak Dongmin , Kim Hyunwoo , Hwang Seonghwan , Yun Hwayoung , Moon Hyung Ryong , Jung Yunjin , Yoo Jin-Wook
Published: October 2022

Although various local anti-inflammatory therapies for ulcerative colitis have been developed, rapid drug elimination from inflamed colitis tissue and off-target side effects reduce their therapeutic efficacy. In this study, we synthesized curcumin (Cur)-loaded hyaluronic acid (HA)-conjugated nanoparticles (Cur-HA-PLGA-NPs) that target inflamed colitis tissue via HA-CD44 interaction with resident colonic epithelial cells and subsequently target activated macrophages for ulcerative colitis therapy. The synthesized spherical Cur-HA-PLGA-NPs showed physicochemical properties similar to those of non-HA-conjugated Cur-PLGA-NPs. HA-PLGA-NPs exhibited selective accumulation in inflamed colitis tissue with minimal accumulation in healthy colon tissue. HA functionalization enhanced targeted drug delivery to intestinal macrophages, significantly increasing HA-PLGA-NP cellular uptake. Importantly, the rectal administration of Cur-HA-PLGA-NPs exhibited better therapeutic efficacy than Cur-PLGA-NPs in animal studies. Histological examination revealed that Cur-HA-PLGA-NPs reduced inflammation with less inflammatory cell infiltration and accelerated recovery with re-epithelialization signs. Our results suggest that Cur-HA-PLGA-NPs are a promising delivery platform for treating ulcerative colitis.
Impact of Guidelines Regarding Dihydropyrimidine Dehydrogenase (DPD) Deficiency Screening Using Uracil-Based Phenotyping on the Reduction of Severe Side Effect of 5-Fluorouracil-Based Chemotherapy: A Propension Score Analysis
Authors: Laures Nicolas , Konecki Céline , Brugel Mathias , Giffard Anne-Lise , Abdelli Naceur , Botsen Damien , Carlier Claire , Gozalo Claire , Feliu Catherine , Slimano Florian , Djerada Zoubir , Bouché Olivier
Published: October 2022

Dihydropyrimidine dehydrogenase (DPD) deficiency is associated with severe fluoropyrimidines-induced toxicity. As of September 2018, French recommendations call for screening for DPD deficiency by plasma uracil quantification prior to all fluoropyrimidine-based chemotherapy. A dose reduction of fluoropyrimidine is recommended when uracil concentration is equal to or greater than 16 ng/mL. This matched retrospective study assessed the impact of DPD screening on the reduction of severe side effects and on the management of DPD-deficient patients. Using a propensity score, we balanced the factors influencing 5-Fluorouracil (5-FU) toxicity. Then, the severity scores (G3 and G4 severity as well as their frequency) of patients who did not benefit from DPD screening were compared with those of patients who benefited from DPD screening for each treatment cycle (from 1 to 4). Among 349 screened patients, 198 treated patients were included. Among them, 31 (15.7%) had DPD deficiency (median uracilemia 19.8 ng/mL (range: 16.1–172.3)). The median toxicity severity score was higher in the unscreened group for each treatment cycle (0 vs. 1, p < 0.001 at each cycle from 1 to 4) as well as the cumulative score during all courses of treatment (p = 0.028). DPD-deficient patients received a significantly lower dose of 5-FU (p < 0.001). This study suggests that pretherapeutic plasmatic uracil assessment, along with 5-FU dosage adjustment, may be beneficial in reducing 5-FU toxicity in real-life patients.
Theranostic Properties of Crystalline Aluminum Phthalocyanine Nanoparticles as a Photosensitizer
Authors: Makarov Vladimir I. , Pominova Daria V. , Ryabova Anastasiya V. , Romanishkin Igor D. , Voitova Arina V. , Steiner Rudolf W. , Loschenov Victor B.
Published: October 2022

The study of phthalocyanines, known photosensitizers, for biomedical applications has been of high research interest for several decades. Of specific interest, nanophotosensitizers are crystalline aluminum phthalocyanine nanoparticles (AlPc NPs). In crystalline form, they are water-insoluble and atoxic, but upon contact with tumors, immune cells, or pathogenic microflora, they change their spectroscopic properties (acquire the ability to fluoresce and become phototoxic), which makes them upcoming agents for selective phototheranostics. Aqueous colloids of crystalline AlPc NPs with a hydrodynamic size of 104 ± 54 nm were obtained using ultrasonic dispersal and centrifugation. Intracellular accumulation and localization of AlPc were studied on HeLa and THP-1 cell cultures and macrophages (M0, M1, M2) by fluorescence microscopy. Crystallinity was assessed by XRD spectroscopy. Time-resolved spectroscopy was used to obtain characteristic fluorescence kinetics of AlPc NPs upon interaction with cell cultures. The photodynamic efficiency and fluorescence quantum yield of AlPc NPs in HeLa and THP-1 cells were evaluated. After entering the cells, AlPc NPs localized in lysosomes and fluorescence corresponding to individual AlPc molecules were observed, as well as destruction of lysosomes and a rapid decrease in fluorescence intensity during photodynamic action. The photodynamic efficiency of AlPc NPs in THP-1 cells was almost 1.8-fold that of the molecular form of AlPc (Photosens). A new mechanism for the occurrence of fluorescence and phototoxicity of AlPc NPs in interaction with cells is proposed.
Angiotensin II Receptor Blockers in the Management of Hypertension in Preventing Cognitive Impairment and Dementia—A Systematic Review
Authors: D’Silva Elvira , Meor Azlan Nur Farah , Zhang Jinwei
Published: October 2022

Hypertension is a known risk factor for cognition-related pathologies including dementia. The National Institute of Health and Care Excellence (NICE) guidelines recommend angiotensin (Ang) II receptor blockers (ARBs) or angiotensin-converting enzyme inhibitors (ACEIs) as a first-line treatment for hypertension. Although both ARBs and ACEIs show neuroprotective effects, ACEIs show contradictory side effects; therefore, ARBs may be a more viable option. However, trials assessing the effects of ARBs on cognition are scarce and conflicting. Therefore, the aim of this review is to conduct a systematic review and synthesise data on the influence of ARBs on cognition and dementia prevention. Five databases were searched from 1992–2022 to produce 13 randomised controlled trials (RCTs) involving 26,907 patients that compared associations of ARBs against placebos or other antihypertensives on cognition or probable dementia with a minimum duration of 3 months. ARBs showed greater cognitive benefits when compared to hydrochlorothiazide (HCTZ), beta blockers (BB), and ACEIs. Our findings showed that although ARBs are superior to some antihypertensives such as ACEIs, thiazide and beta blockers, they made no difference in comparison to the placebo in all but one sample of patients. The positive effects on cognitive performances are equal to calcium channel blockers (CCBs) and lower than statin. The neuroprotective effects of ARBs are also more beneficial when ARBs are taken at the same time as a statin. Due to these inconsistencies, robust conclusions cannot be made. Future trials are warranted and, if successful, could have positive economic implications and consequently improve quality of life.
Gallium Protoporphyrin Liquid Crystalline Lipid Nanoparticles: A Third-Generation Photosensitizer against Pseudomonas aeruginosa Biofilms
Authors: Awad Muhammed , Barnes Timothy J. , Thomas Nicky , Joyce Paul , Prestidge Clive A.
Published: October 2022

The looming antimicrobial resistance pandemic has encouraged the investigation of antimicrobial photodynamic therapy (aPDT) as a promising technology to combat recalcitrant bacterial infections caused by antibiotic resistant strains. Here, we report on the optimization and effective application of gallium protoporphyrin liquid crystalline lipid nanoparticles (GaPP-LCNP) as a photosensitizer for aPDT against the Gram-negative bacteria P. aeruginosa in both planktonic and biofilm modes of growth. LCNP significantly enhanced the performance of GaPP as photosensitizer by two-fold, which was correlated with higher antibacterial activity, reducing the viability of planktonic P. aeruginosa by 7 log10 using 0.8 µM GaPP-LCNP and a light dose of 17 J.cm−2. Importantly, GaPP-LCNP also reduced the viability of biofilms by 6 log10 at relatively low light dose of 34.2 J.cm−2 using only 3 µM GaPP-LCNP. The high antibiofilm activity of GaPP-LCNP at low GaPP-LCNP dose indicated the high efficiency and safety profile of GaPP-LCNP as a promising platform for photodynamic inactivation of recalcitrant infections.
Autologous Marrow Mesenchymal Stem Cell Driving Bone Regeneration in a Rabbit Model of Femoral Head Osteonecrosis
Authors: Mastrolia Ilenia , Giorgini Andrea , Murgia Alba , Loschi Pietro , Petrachi Tiziana , Rasini Valeria , Pinelli Massimo , Pinto Valentina , Lolli Francesca , Chiavelli Chiara , Grisendi Giulia , Baschieri Maria Cristina , Santis Giorgio De , Catani Fabio , Dominici Massimo , Veronesi Elena
Published: October 2022

Osteonecrosis of the femoral head (ONFH) is a progressive degenerative disease that ultimately requires a total hip replacement. Mesenchymal stromal/stem cells (MSCs), particularly the ones isolated from bone marrow (BM), could be promising tools to restore bone tissue in ONFH. Here, we established a rabbit model to mimic the pathogenic features of human ONFH and to challenge an autologous MSC-based treatment. ON has been originally induced by the synergic combination of surgery and steroid administration. Autologous BM-MSCs were then implanted in the FH, aiming to restore the damaged tissue. Histological analyses confirmed bone formation in the BM-MSC treated rabbit femurs but not in the controls. In addition, the model also allowed investigations on BM-MSCs isolated before (ON-BM-MSCs) and after (ON+BM-MSCs) ON induction to dissect the impact of ON damage on MSC behavior in an affected microenvironment, accounting for those clinical approaches foreseeing MSCs generally isolated from affected patients. BM-MSCs, isolated before and after ON induction, revealed similar growth rates, immunophenotypic profiles, and differentiation abilities regardless of the ON. Our data support the use of ON+BM-MSCs as a promising autologous therapeutic tool to treat ON, paving the way for a more consolidated use into the clinical settings.
Topical Antibiotic Prophylaxis and Intravitreal Injections: Impact on the Incidence of Acute Endophthalmitis—A Nationwide Study in France from 2009 to 2018
Authors: Baudin Florian , Benzenine Eric , Mariet Anne-Sophie , Ghezala Inès Ben , Bron Alain M. , Daien Vincent , Gabrielle Pierre-Henry , Quantin Catherine , Creuzot-Garcher Catherine
Published: October 2022

Background: The dramatic increase in intravitreal injections (IVTs) has been accompanied by a greater need for safer procedures. The ongoing debate about topical antibiotic prophylaxis after IVTs emphasizes the importance of large-scale studies. We aimed to study the role of topical antibiotic prophylaxis in reducing the risk of acute endophthalmitis after IVTs. Methods: Population-based cohort study, in France, from 2009 to 2018, including all French patients receiving IVTs of corticosteroids or anti-VEGF agents. Results: A total of 5,291,420 IVTs were performed on 605,434 patients. The rate of topical antibiotic prophylaxis after IVTs progressively decreased during the study period, with a sharp drop in 2014 (from 84.6% in 2009 to 27.4% in 2018). Acute endophthalmitis occurred in 1274 cases (incidence rate = 0.0241%). Although antibiotic prophylaxis did not alter the risk of endophthalmitis (p = 0.06), univariate analysis showed an increased risk after fluoroquinolone and aminoglycoside prophylaxis. This increased risk was not found in multivariate analysis. However, we observed an increased risk related to the use of fixed combinations of fluoroquinolones and aminoglycosides with corticosteroids (IRR = 1.89; 95% CI = 1.57–2.27%, antibiotics combined with corticosteroids). Conclusion: These results are consistent with the literature. Endophthalmitis rates after IVTs did not decrease with topical antibiotic prophylaxis. The use of a combination of antibiotics and corticosteroids doubles the risk of endophthalmitis and should be avoided. Avoiding antibiotic prophylaxis would reduce the costs and the potential risks of antibiotic resistance.
The Anti-Obesity Potential of Superparamagnetic Iron Oxide Nanoparticles against High-Fat Diet-Induced Obesity in Rats: Possible Involvement of Mitochondrial Biogenesis in the Adipose Tissues
Authors: Alsenousy Aisha H. A. , El-Tahan Rasha A. , Ghazal Nesma A. , Piñol Rafael , Millán Angel , Ali Lamiaa M. A. , Kamel Maher A.
Published: October 2022

Background: Obesity is a pandemic disease that is rapidly growing into a serious health problem and has economic impact on healthcare systems. This bleak image has elicited creative responses, and nanotechnology is a promising approach in obesity treatment. This study aimed to investigate the anti-obesity effect of superparamagnetic iron oxide nanoparticles (SPIONs) on a high-fat-diet rat model of obesity and compared their effect to a traditional anti-obesity drug (orlistat). Methods: The obese rats were treated daily with orlistat and/or SPIONs once per week for 8 weeks. At the end of the experiment, blood samples were collected for biochemical assays. Then, the animals were sacrificed to obtain white adipose tissues (WAT) and brown adipose tissues (BAT) for assessment of the expression of thermogenic genes and mitochondrial DNA copy number (mtDNA-CN). Results: For the first time, we reported promising ameliorating effects of SPIONs treatments against weight gain, hyperglycemia, adiponectin, leptin, and dyslipidemia in obese rats. At the molecular level, surprisingly, SPIONs treatments markedly corrected the disturbed expression and protein content of inflammatory markers and parameters controlling mitochondrial biogenesis and functions in BAT and WAT. Conclusions: SPIONs have a powerful anti-obesity effect by acting as an inducer of WAT browning and activator of BAT functions.
Quantum Dots Mediated Imaging and Phototherapy in Cancer Spheroid Models: State of the Art and Perspectives
Authors: Dirheimer Luca , Pons Thomas , Marchal Frédéric , Bezdetnaya Lina
Published: October 2022

Quantum Dots (QDs) are fluorescent nanoparticles known for their exceptional optical properties, i.e., high fluorescence emission, photostability, narrow emission spectrum, and broad excitation wavelength. These properties make QDs an exciting choice for bioimaging applications, notably in cancer imaging. Challenges lie in their ability to specifically label targeted cells. Numerous studies have been carried out with QDs coupled to various ligands like peptides, antibodies, aptamers, etc., to achieve efficient targeting. Most studies were conducted in vitro with two-dimensional cell monolayers (n = 8902) before evolving towards more sophisticated models. Three-dimensional multicellular tumor models better recapitulate in vivo conditions by mimicking cell-to-cell and cell-matrix interactions. To date, only few studies (n = 34) were conducted in 3D in vitro models such as spheroids, whereas these models could better represent QDs behavior in tumors compared to monolayers. Thus, the purpose of this review is to present a state of the art on the studies conducted with Quantum Dots on spheroid models for imaging and phototherapy purposes.
Enhanced Production of ECM Proteins for Pharmaceutical Applications Using Mammalian Cells and Sodium Heparin Supplementation
Authors: Garcia-Pardo Javier , Montané Sergi , Avilés Francesc Xavier , Tanco Sebastian , Lorenzo Julia
Published: October 2022

The yields of soluble ECM proteins recombinantly produced with mammalian cells can be significantly enhanced by exploiting the stabilizing properties of heparin. Here, we propose a simple and straightforward scalable protocol for the mammalian cell production of ECM proteins with affinity for heparin, using heparin as a supplement. As proof of concept, we have demonstrated the high-level expression of four biomedically relevant human enzymes such as carboxypeptidase Z (CPZ), carboxypeptidase A6 (CPA6), beta-galactoside alpha-2,6-sialyltransferase 2 (ST6GAL1) and thrombin-activable fibrinolysis inhibitor (TAFI). We found a strong linear correlation between the isoelectric point (pI) of a protein and the improvement in protein expression levels upon heparin addition, providing a reference for selecting novel protein targets that would benefit from heparin supplementation. Finally, we demonstrated the compatibility of this approach with a three-step purification strategy that includes an initial heparin affinity purification step. Using CPZ as a representative example, we performed a preparative purification of this enzyme. The purified protein is enzymatically active and can be used for pharmaceutical applications as well as for high-throughput functional and structural studies.
Nano-Dry-Melting: A Novel Technology for Manufacturing of Pharmaceutical Amorphous Solid Dispersions
Authors: Hermeling Malin , Nueboldt Christoph , Heumann Roman , Hoheisel Werner , Breitkreutz Joerg
Published: October 2022

Amorphous solid dispersions (ASD) are one of the most prominent formulation approaches to overcome bioavailability issues that are often presented by new poorly soluble drug candidates. State-of-the art manufacturing techniques include hot melt extrusion and solvent-based methods like spray drying. The high thermal and mechanical shear stress during hot melt extrusion, or the use of an organic solvent during solvent-based methods, are examples of clear drawbacks for those methods, limiting their applicability for certain systems. In this work a novel process technology is introduced, called Nano-Dry-Melting (NDM), which can provide an alternative option for ASD manufacturing. NDM consists of a comminution step in which the drug is ground to nanosize and a drying step provides a complete amorphization of the system at temperatures below the melting point. Two drug–polymer systems were prepared using NDM with a wet media mill and a spray dryer and analyzed regarding their degree of crystallinity using XRD analysis. Feasibility studies were performed with indomethacin and PVP. Furthermore, a “proof-of-concept” study was conducted with niclosamide. The experiments successfully led to amorphous samples at temperatures of about 50 K below the melting point within seconds of heat exposition. With this novel, solvent-free and therefore “green” production technology it is feasible to manufacture ASDs even with those drug candidates that cannot be processed by conventional process technologies.
Advances in Pharmacy Practice: A Look towards the Future
Authors: Atkinson Jeffrey
Published: October 2022

This review looks at the factors that may influence practice in the future. Transformation could occur at 3 levels. Firstly, the traditional profession of the pharmacist as a dispenser of medicines is expanding. Secondly, the pharmacist’s activities are progressing into new healthcare fields. Thirdly, other changes are stimulated by global developments. This review may be helpful for pharmacy and healthcare leaders looking at the future configuration and aims of their pharmacy services.
A Vitalist Shoal in the Mechanist Tide: Art, Nature, and 17th-Century Science
Authors: Shaheen Jonathan L.
Published: October 2022

This paper reconstructs Margaret Cavendish’s theory of the metaphysics of artifacts. It situates her anti-mechanist account of artifactual production and the art-nature distinction against a background of Aristotelian, Scholastic, and mechanist theories. Within this broad context, it considers what Cavendish thinks artisans can actually do, grounding her terminological stipulation that there is no genuine generation in nature in a commitment to natural and artistic production as the mere rearrangement of bodies. Bodies themselves are identified, in a conceptually Ockhamist manner, with their figures, so that the resulting theory of mere rearrangement is Scholastically respectable. The paper also offers literal interpretations, focused narrowly on the philosophical content of her theories of art and artifacts, of her claims that art concerns only “nature’s sporting or playing actions”, that its products are “deformed and defective”, and that they are “at best …mixt or hermaphroditical."
Not All Computational Methods Are Effective Methods
Authors: Sprevak Mark
Published: October 2022

An effective method is a computational method that might, in principle, be executed by a human. In this paper, I argue that there are methods for computing that are not effective methods. The examples I consider are taken primarily from quantum computing, but these are only meant to be illustrative of a much wider class. Quantum inference and quantum parallelism involve steps that might be implemented in multiple physical systems, but cannot be implemented, or at least not at will, by an idealised human. Recognising that not all computational methods are effective methods is important for at least two reasons. First, it is needed to correctly state the results of Turing and other founders of computation theory. Turing is sometimes said to have offered a replacement for the informal notion of an effective method with the formal notion of a Turing machine. I argue that such a view only holds under limited circumstances. Second, not distinguishing between computational methods and effective methods can lead to mistakes when quantifying over the class of all possible computational methods. Such quantification is common in philosophy of mind in the context of thought experiments that explore the limits of computational functionalism. I argue that these ‘homuncular’ thought experiments should not be treated as valid.
Efficient Access of Phenyl-Spaced 5,5′-Bridged Dinuclear Ruthenium Metal Complexes and the Effect of Dynamic Ligand Exchange on Catalysis
Authors: Lämmle Martin , Volk Steffen , Klinkerman Madelyn , Müßler Marius , Mengele Alexander K. , Rau Sven
Published: October 2022

Herein, we present the synthesis, characterization, and light-driven hydrogen evolution activity of two dinuclear Ru-Pt complexes, Rup(ph)pPtX2 (X = Cl, I), comprising a new phenyl-spaced 5,5′-bis-phenanthroline p(ph)p bridging ligand. The two complexes only differ in the nature of the halide ligand at the catalytic center. Structural, photophysical, electrochemical, as well as photochemical characterization techniques revealed that the variations of single components of the intramolecular system provide a strong influence on the stability even in non-catalytic conditions. Interestingly, varying electron density at the catalytic center, mainly influenced by the coordinating halide at the catalytic center, as shown by 195Pt NMR spectroscopy, strongly influences the photocatalytic efficiency. Furthermore, intensive investigations on the potential catalytic mechanism showed that small structural variations (e.g., halide exchange) not only affect catalytic activity but can also switch the main catalytic mechanism from an initially molecular one to a fully heterogeneous, colloid-driven hydrogen evolution.
Measurement of the Attenuation Coefficient in Fresh Water Using the Adjacent Frame Difference Method
Authors: Yang Gang , Tian Zhaoshuo , Bi Zongjie , Cui Zihao , Sun Fenghao , Liu Qingcao
Published: October 2022

The attenuation coefficient of a water body is one of the important factors for describing its features. However, its remote measurement in real time is still a challenge. In this paper, we demonstrated a novel method to realize real-time remote measurements of the attenuation coefficient of fresh water using flash imaging lidar based on the adjacent frame difference (AFD) method and a water body backscattering model. In general, we firstly investigated the relationship between the backscattering intensity and the attenuation coefficient based on the backscattering model of the water body. Then, the backscattering intensity at the front and back edges of the range-gate obtained by the AFD method was brought into this relationship to obtain the attenuation coefficient. Experiments on the measurements of the average attenuation coefficient of the 532 nm laser in fresh water at 3–8 m were further carried out using our self-developed flash lidar with the AFD method. The acquired water attenuation coefficients were 0.1334±0.02 and 0.1382±0.03 with a delay step time of 1 ns and 2 ns in the AFD method, respectively. We compared these values to the one achieved following the conventional Beer–Lambert law (0.1330±0.02), and they matched well with each other. These results fully illustrated the feasibility and reliability of the proposed method for measuring the attenuation coefficient of water bodies.
Decoding-Order-Based Power Allocation (DOPA) Scheme for Non-Orthogonal Multiple Access (NOMA) Visible Light Communication Systems
Authors: Alqahtani Ali H. , Almohimmah Esam M. , Alresheedi Mohammed T. , Abas Ahmad Fauzi , Qidan Ahmad-Adnan , Elmirghani Jaafar
Published: October 2022

Non-orthogonal multiple access (NOMA) is an effective multiple access scheme that can be used to improve considerably the spectral efficiency of indoor downlink visible light communication (VLC) systems. However, NOMA suffers from inevitable multi-user interference which degrades the system performance. In this paper, a NOMA scheme is applied in a downlink VLC system and the impact of the multi-user interference on the system performance is studied. A closed-form expression for the user symbol error rate (SER) is derived and a decoding-order-based power allocation (DOPA) method is proposed to reduce the multi-user interference and find the optimal power allocation that minimizes the SER. The significance of the proposed schemes is demonstrated by simulation. The results show that the proposed DOPA method is able to reduce effectively the multi-user interference and provide more sum rate in comparison with benchmarking schemes such as the gain ratio PA (GRPA) and the normalized gain difference PA (NGDPA) methods.
Multi-Wavelength Spatiotemporal Mode-Locked Fiber Laser at 1.55 μm
Authors: Ma Ze-Long , Zhao Tian-Xu , Hong Wei-Yi , Cui Hu , Luo Zhi-Chao , Xu Wen-Cheng , Luo Ai-Ping
Published: October 2022

We propose and demonstrate an effective and simple multi-wavelength spatiotemporal mode-locked (STML) laser. The laser introduces a multimode interference (MMI) effect using the core diameter mismatch of the fibers, thus enabling simultaneous mode-locking at multiple wavelengths. For the multi-wavelength mode-locking, each wavelength exists a matching pulse sequence, and different wavelengths can possess the same pulse state or different states. Meanwhile, at the dual-wavelength operation, by adjusting the polarization controllers, not only the central wavelength can be continuously adjusted from 1552.93 nm to 1572.64 nm, but also the wavelength interval can be adjusted from 2 nm to 19 nm, which benefits from the tunability of the MMI effect. Moreover, the spectral channels can be consecutively increased from 2 to 4. The obtained results provide a platform for understanding and studying the multi-wavelength STML properties. This flexible all-fiber laser has great potential for applications in the fields of optical signal processing, fiber sensing, and information coding.
A Proposition to Correct Infrared Emissivity Measurements for Curved Surface Targets Based on the Irradiation Reflection Method
Authors: Zhang Puyousen , Li Yiwen , Li Yao , Chen Ge , Hua Weizhuo
Published: October 2022

In this paper, a measurement correction method is proposed to correct the error of the irradiation reflection method when measuring the emissivity of curved surface targets. First, by introducing an angle parameter related to the target surface shape, the formulas of emissivity measurements for curved surface targets under various types of radiation sources were obtained. Then, the variation law of these emissivity measurement formulas was analyzed, the concept of the measurement correction factor was proposed, and then a unified correction measurement formula was obtained. Finally, a scene of measuring the emissivity of a curved surface target based on the irradiation reflection method was simulated. Different target emissivities and different distances between the radiation source and target were set, and the errors between the measured and corrected emissivities were compared. The results revealed that the proposed method could effectively correct the error caused by the curvature of the target surface when measuring its emissivity by the irradiation reflection method. This is of great significance to expanding the application scope of the irradiation reflection method and improving the measurement accuracy.
Cross-Domain Resource Allocation Scheme with Unified Control Architecture in Software Defined Optical Access Network
Authors: Bao Bowen , Yao Qiuyan , Li Chao , Sun Zhengjie , Li Xiangyue , Bai Wei , Yang Hui , Liu Sheng , Li Yunbo
Published: October 2022

With the rapid development of communication and the rise of new network services, the resource provisioning of the optical access network becomes more significant than before, especially under the multiple-domain networking situation with the requirement of cross-domain service support. For the sake of high resource utilization to establish more connections of services, this paper proposes a cross-domain resource allocation (CDRA) scheme in a software-defined optical access network to meet the huge bandwidth supporting the requirement of new network services. To achieve this purpose, the global evaluation strategy with the consideration of the traffic situation in each node in its domain is presented in the CDRA scheme, and its interaction process makes decisions for comprehensive optimal resource allocation by integrating radio resources and optical aggregation resources of the entire access network. Furthermore, to manage the resources among multiple domains and support the interaction process of the CDRA scheme, a cross-domain unified control architecture is firstly upgraded by using software-defined networking technology, which includes the further design of CDRA function modules with the global evaluation strategy. The simulation results verify the feasibility of the upgraded architecture and further show that the proposed CDRA scheme can effectively decrease the blocking probability with a 29.35% improvement, balance the network load, and enhance the utilization of network resources of the network.
Synchronization of Optomechanical Oscillators in Coupled 1D Optomechanical Crystal Nanobeam Cavities
Authors: Liu Yang , Gao Fei , Yang Daquan , Wang Aiqiang , Zhou Mengchen , Li Shanchuang , Gao Lu , Zhang Ze
Published: October 2022

We proposed a new optomechanical system (OMS) based on parallel suspended one-dimensional optomechanical crystal (1D-OMC) nanobeam cavities for optomechanical synchronization. The optomechanical oscillators (OMOs) were spaced apart by an air-slot gap and coupled through optical radiation fields. The numerical simulation showed that the evolution process of 1D-OMC nanobeam cavities to mechanical synchronization could be divided into three clear stages. The synchronization of two mechanical breathing modes at 5.8846 GHz was achieved by using a single laser source. Finally, we investigated the relationship between the threshold power and detuning of an input laser for self-sustaining and synchronization states. Such chip-based structures hold great potential for large-scale synchronized oscillator networks.
Gellan Gum in Wound Dressing Scaffolds
Authors: Feketshane Zizo , Alven Sibusiso , Aderibigbe Blessing Atim
Published: October 2022

Several factors, such as bacterial infections, underlying conditions, malnutrition, obesity, ageing, and smoking are the most common issues that cause a delayed process of wound healing. Developing wound dressings that promote an accelerated wound healing process and skin regeneration is crucial. The properties of wound dressings that make them suitable for the acceleration of the wound healing process include good antibacterial efficacy, excellent biocompatibility, and non-toxicity, the ability to provide a moist environment, stimulating cell migration and adhesion, and providing gaseous permeation. Biopolymers have demonstrated features appropriate for the development of effective wound dressing scaffolds. Gellan gum is one of the biopolymers that has attracted great attention in biomedical applications. The wound dressing materials fabricated from gellan gum possess outstanding properties when compared to traditional dressings, such as good biocompatibility, biodegradability, non-toxicity, renewability, and stable nature. This biopolymer has been broadly employed for the development of wound dressing scaffolds in different forms. This review discusses the physicochemical and biological properties of gellan gum-based scaffolds in the management of wounds.
Poly(Lactic Acid) Block Copolymers with Poly(Hexylene Succinate) as Microparticles for Long-Acting Injectables of Risperidone Drug
Authors: Chrysafi Iouliana , Nanaki Stavroula , Zamboulis Alexandra , Kostoglou Margaritis , Pavlidou Eleni , Bikiaris Dimitrios N.
Published: October 2022

In the present work, Risperidone microparticles from poly(lactic acid)/poly(hexylene succinate) (PLA-b-PHSu) block copolymers in different ratios, 95/05, 90/10 and 80/20 w/w, were examined as long-acting injectable formulations. Nuclear magnetic resonance (NMR) was used to verify the successful synthesis of copolymers. Enzymatic hydrolysis showed an increase in weight loss as the content of PHSu increased, while the cytotoxicity studies confirmed the biocompatibility of the copolymers. The polyesters were further used to encapsulate Risperidone by spray drying. The drug-loaded microparticles were studied by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction (XRD). SEM microphotographs confirmed that spherically shaped microparticles were prepared with sizes about 5–12 μm, while XRD and differential scanning calorimetry (DSC) studies evidenced that Risperidone was encapsulated in amorphous form. The drug loading and the entrapment efficiency of Risperidone were studied as well as the in vitro release from the prepared microparticles. As the content of PHSu increased, a higher release of Risperidone was observed, with PLA-b-PHSu 80/20 w/w succeeding to release 100% of RIS within 12 days. According to theoretical modeling, the kinetics of RIS release from PLA-b-PHSu microparticles is complex, governed by both diffusion and polymer erosion.
Magnetic Field Influence on the Microwave Characteristics of Composite Samples Based on Polycrystalline Y-Type Hexaferrite
Authors: Kolev Svetoslav , Georgieva Borislava , Koutzarova Tatyana , Krezhov Kiril , Ghelev Chavdar , Kovacheva Daniela , Vertruyen Benedicte , Closset Raphael , Tran Lan Maria , Babij Michal , Zaleski Andrzej J.
Published: October 2022

Here, we report results on the magnetic and microwave properties of polycrystalline Y-type hexaferrite synthesized by sol-gel auto-combustion and acting as a filler in a composite microwave-absorbing material. The reflection losses in the 1–20 GHz range of the Y-type hexaferrite powder dispersed homogeneously in a polymer matrix of silicon rubber were investigated in the absence and in the presence of a magnetic field. A permanent magnet was used with a strength of 1.4 T, with the magnetic force lines oriented perpendicularly to the direction of the electromagnetic wave propagation. In the case of using an external magnetic field, an extraordinary result was observed. The microwave reflection losses reached a maximum value of 35.4 dB at 5.6 GHz in the Ku-band without a magnetic field and a maximum value of 21.4 dB at 8.2 GHz with the external magnetic field applied. The sensitivity of the microwave properties of the composite material to the external magnetic field was manifested by the decrease of the reflected wave attenuation. At a fixed thickness, tm, of the composite, the attenuation peak frequency can be adjusted to a certain value either by changing the filling density or by applying an external magnetic field.
Nanoscopic Characterization of Starch Biofilms Extracted from the Andean Tubers Ullucus tuberosus, Tropaeolum tuberosum, Oxalis tuberosa, and Solanum tuberosum
Authors: Pico Cynthia , De la Vega Jhomara , Tubón Irvin , Arancibia Mirari , Casado Santiago
Published: October 2022

The replacement of synthetic polymers by starch biofilms entails a significant potentiality. They are non-toxic materials, biodegradable, and relatively easy to gather from several sources. However, various applications may require physicochemical properties that might prevent the use of some types of starch biofilms. Causes should be explored at the nanoscale. Here we present an atomic force microscopy surface analysis of starch biofilms extracted from the Andean tubers melloco (Ullucus tuberosus), mashua (Tropaeolum tuberosum), oca (Oxalis tuberosa), and potato (Solanum tuberosum) and relate the results to the macroscopic effects of moisture content, water activity, total soluble matter, water vapor permeability, elastic properties, opacity and IR absorption. Characterization reveals important differences at the nanoscale between the starch-based biofilms examined. Comparison permitted correlating macroscopic properties observed to the topography and tapping phase contrast segregation at the nanoscale. For instance, those samples presenting granular topography and disconnected phases at the nanoscale are associated with less elastic strength and more water molecule affinity. As an application example, we propose using the starch biofilms developed as a matrix to dispose of mouthwash and discover that melloco films are quite appropriate for this purpose.
Effect of Extraction Ingredients on the Conformation and Stability of Silk Sericin (SS)
Authors: Muindi Munguti Peter , Lee Ji Hae , Kweon HaeYong , Kasina Muo
Published: October 2022

Silk sericin (SS) has different physicochemical properties depending on the extraction technique. In this study, SS was isolated in the presence of ingredients, including 5 to 10% ethanol (EtOH) and 5 to 10% glycine. Furthermore, temperature conditions of 80 °C, 100 °C, and 120 °C were used for 1, 3, and 5 h to evaluate the extraction rates. The extraction, gelation, structural, and cytotoxicity properties of SS extracted under different conditions were investigated. Extraction at 100 °C and 120 °C were found to have the highest SS yield, with 80 °C being the lowest. SS isolated at 100 °C and 120 °C for 1 and 3 h in water, and EtOH gelled at 4 °C in 2 to 3 days and 37 °C in 40 min. Glycine SS extracts were obtained at 100 °C and 120 °C for 1 h, gelled at 4 °C for 20 days and 37 °C for 16 h. SS was observed at 80 °C, with no gelation occurring. Glycine SS extracts obtained for 3, and 5 h at 120 °C showed no gelation. Circular dichroism (CD) results show glycine in SS induces α-helix and random coil structure. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and fast performance liquid chromatography (FPLC) were used to quantify the molecular weight distribution at 63 and 70 kDa, respectively. The MMT assay (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) revealed no cytotoxicity in macrophage RAW 264.7 cells treated with this method SS; these findings present the significance and possibility of using selected extraction ingredients in SS that allow for the application of native SS at an initial extraction viscosity.
Sol-Gel Coatings with Azofoska Fertilizer Deposited onto Pea Seeds
Authors: Borak Beata
Published: October 2022

Pure silica sol obtained by hydrolysis of tetraethoxysilane and the same silica sol doped with fertilizer Azofoska were used to cover the surface of pea seeds. The surface state of the coated seeds (layer continuity, thickness, elemental composition) was studied by a scanning electron microscope (SEM) and energy dispersive X-ray (EDX) detector. Different conditions such as sol mixing method, seed immersion time, effect of diluting the sol with water, and ethanol (EtOH) were studied to obtain thin continuous coatings. The coated seeds were subjected to a germination and growth test to demonstrate that the produced SiO2 coating did not inhibit these processes; moreover, the presence of fertilizer in the coating structure facilitates the development of the seedling. The supply of nutrients directly to the grain’s vicinity contributes to faster germination and development of seedlings. This may give the developing plants an advantage in growth over other undesirable plant species. These activities are in the line with the trends of searching for technologies increasing yields without creating an excessive burden on the natural environment.
Synthesis of Optically and Redox Active Polyenaminones from Diamines and α,α’-Bis[(dimethylamino)methylidene]cyclohexanediones
Authors: Štanfel Urša , Kotnik Tomaž , Ričko Sebastijan , Grošelj Uroš , Štefane Bogdan , Pirnat Klemen , Žagar Ema , Genorio Boštjan , Svete Jurij
Published: October 2022

New oligo- and polyenaminones with Mw ~ 7–50 KDa were prepared in high yields by transaminative amino-enaminone polymerization of regioisomeric bis[(dimethylamino)methylidene]cyclohexanediones with alkylene and phenylenediamines. The polymers obtained are practically insoluble in aqueous and organic solvents and exhibit film-forming properties, UV light absorption at wavelengths below 500 nm, and redox activity. These properties indicate a promising application potential of these polymers, which could find use in optical and optoelectronic applications and in energy storage devices.
Colorimetric Indicators Based on Anthocyanin Polymer Composites: A Review
Authors: Kossyvaki Despoina , Contardi Marco , Athanassiou Athanassia , Fragouli Despina
Published: October 2022

This review explores the colorimetric indicators based on anthocyanin polymer composites fabricated in the last decade, in order to provide a comprehensive overview of their morphological and compositional characteristics and their efficacy in their various application fields. Notably, the structural properties of the developed materials and the effect on their performance will be thoroughly and critically discussed in order to highlight their important role. Finally, yet importantly, the current challenges and the future perspectives of the use of anthocyanins as components of colorimetric indicator platforms will be highlighted, in order to stimulate the exploration of new anthocyanin sources and the in-depth investigation of all the possibilities that they can offer. This can pave the way for the development of high-end materials and the expansion of their use to new application fields.
Assessment of Oxaliplatin-Loaded Iodine Nanoparticles for Chemoradiotherapy of Human Colorectal Cancer (HT-29) Cells
Authors: Rasouli Naser , Shahbazi-Gahrouei Daryoush , Hematti Simin , Baradaran Behzad , Salehi Roya , Varshosaz Jaleh , Jafarizad Abbas
Published: October 2022

Colorectal cancer is highly prevalent worldwide and has significant morbidity and mortality in humans. High-atomic-number nanoparticles such as iodine can act as X-rays absorbers to increase the local dose. The synthesis and fabrication of oxaliplatin-loaded iodine nanoparticles, their characterization, cell toxicity, radiosensitivity, cell apoptosis, and cell cycle assay in human colorectal cancer (HT-29) cells are investigated. Results show that the synthesis of a new iodine nanoparticle, polymerized triiodobenzene coated with chitosan and combined with oxaliplatin as a chemotherapeutic drug, performed well in vitro in an intracellular radiosensitizer as chemoradiotherapy agent in HT-29 cell lines. Findings also show that the INPs alone have no impact on cell cycle development and apoptosis. In contrast, oxaliplatin-loaded INPs along with 2 and 6 MV radiation doses produced more apoptosis. The interaction of INPs with mega-voltage photon energies is the cause of a major radiosensitization enhancement in comparison to radiation alone. Furthermore, results show that INPs may work as radiosensitization nanoprobe agents in the treatment of HT-29 cells due to their effect on increasing radiation dose absorption. Overall, iodine nanoparticles may be used in the treatment of colorectal cancers in clinical studies.