bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcriptomic Bioinformatic Analyses of Atria Uncover Involvement of Pathways Related to Strain and Post-translational Modification of Collagen in Increased Atrial Fibrillation Vulnerability in Intensely Exercised Mice
Authors: Oh Yena , Yang Sibao , Liu Xueyan , Jana Sayantan , Izaddoustdar Farzad , Gao Xiaodong , Debi Ryan , Kim Dae-Kyum , Kim Kyoung-Han , Yang Ping , Kassiri Zamaneh , Lakin Robert , Backx Peter H.
Published: December 2020

Atrial Fibrillation (AF) is the most common supraventricular tachyarrhythmia that is typically associated with cardiovascular disease (CVD) and poor cardiovascular health. Paradoxically, endurance athletes are also at risk for AF. While it is well-established that persistent AF is associated with atrial fibrosis, hypertrophy and inflammation, intensely exercised mice showed similar adverse atrial changes and increased AF vulnerability, which required tumor necrosis factor (TNF) signaling, even though ventricular structure and function improved. To identify some of the molecular factors underlying the chamber-specific and TNF-dependent atrial changes induced by exercise, we performed transcriptome analyses of hearts from wild-type and TNF-knockout mice following exercise for 2 days, 2 or 6 weeks of exercise. Consistent with the central role of atrial stretch arising from elevated venous pressure in AF promotion, all 3 time points were associated with differential regulation of genes in atria linked to mechanosensing (focal adhesion kinase, integrins and cell-cell communications), extracellular matrix (ECM) and TNF pathways, with TNF appearing to play a permissive, rather than causal, role in gene changes. Importantly, mechanosensing/ECM genes were only enriched, along with tubulin- and hypertrophy-related genes after 2 days of exercise while being downregulated at 2 and 6 weeks, suggesting that early reactive strain-dependent remodeling with exercise yields to compensatory adjustments. Moreover, at the later time points, there was also downregulation of both collagen genes and genes involved in collagen turnover, a pattern mirroring aging-related fibrosis. By comparison, twofold fewer genes were differentially regulated in ventricles vs. atria, independently of TNF. Our findings reveal that exercise promotes TNF-dependent atrial transcriptome remodeling of ECM/mechanosensing pathways, consistent with increased preload and atrial stretch seen with exercise. We propose that similar preload-dependent mechanisms are responsible for atrial changes and AF in both CVD patients and athletes.
Extensive Simulated Diving Aggravates Endothelial Dysfunction in Male Pro-atherosclerotic ApoE Knockout Rats
Authors: Berenji Ardestani Simin , Matchkov Vladimir V. , Hansen Kasper , Jespersen Nichlas Riise , Pedersen Michael , Eftedal Ingrid
Published: December 2020

Introduction

The average age of the diving population is rising, and the risk of atherosclerosis and cardiovascular disease in divers are accordingly increasing. It is an open question whether this risk is altered by diving per se. In this study, we examined the effect of 7-weeks simulated diving on endothelial function and mitochondrial respiration in atherosclerosis-prone rats.

Methods

Twenty-four male ApoE knockout (KO) rats (9-weeks-old) were fed a Western diet for 8 weeks before 12 rats were exposed to simulated heliox dry-diving in a pressure chamber (600 kPa for 60 min, decompression of 50 kPa/min). The rats were dived twice-weekly for 7 weeks, resulting in a total of 14 dives. The remaining 12 non-diving rats served as controls. Endothelial function of the pulmonary and mesenteric arteries was examined in vitro using an isometric myograph. Mitochondrial respiration in cardiac muscle tissues was measured using high-resolution respirometry.

Results and Conclusion

Both ApoE KO diving and non-diving rats showed changes in endothelial function at the end of the intervention, but the extent of these changes was larger in the diving group. Altered nitric oxide signaling was primarily involved in these changes. Mitochondrial respiration was unaltered. In this pro-atherosclerotic rat model of cardiovascular changes, extensive diving appeared to aggravate endothelial dysfunction rather than promote adaptation to oxidative stress.
3D Compressed Convolutional Neural Network Differentiates Neuromyelitis Optical Spectrum Disorders From Multiple Sclerosis Using Automated White Matter Hyperintensities Segmentations
Authors: Wang Zhuo , Yu Zhezhou , Wang Yao , Zhang Huimao , Luo Yishan , Shi Lin , Wang Yan , Guo Chunjie
Published: December 2020

Background

Magnetic resonance imaging (MRI) has a wide range of applications in medical imaging. Recently, studies based on deep learning algorithms have demonstrated powerful processing capabilities for medical imaging data. Previous studies have mostly focused on common diseases that usually have large scales of datasets and centralized the lesions in the brain. In this paper, we used deep learning models to process MRI images to differentiate the rare neuromyelitis optical spectrum disorder (NMOSD) from multiple sclerosis (MS) automatically, which are characterized by scattered and overlapping lesions.

Methods

We proposed a novel model structure to capture 3D MRI images’ essential information and converted them into lower dimensions. To empirically prove the efficiency of our model, firstly, we used a conventional 3-dimensional (3D) model to classify the T2-weighted fluid-attenuated inversion recovery (T2-FLAIR) images and proved that the traditional 3D convolutional neural network (CNN) models lack the learning capacity to distinguish between NMOSD and MS. Then, we compressed the 3D T2-FLAIR images by a two-view compression block to apply two different depths (18 and 34 layers) of 2D models for disease diagnosis and also applied transfer learning by pre-training our model on ImageNet dataset.

Results

We found that our models possess superior performance when our models were pre-trained on ImageNet dataset, in which the models’ average accuracies of 34 layers model and 18 layers model were 0.75 and 0.725, sensitivities were 0.707 and 0.708, and specificities were 0.759 and 0.719, respectively. Meanwhile, the traditional 3D CNN models lacked the learning capacity to distinguish between NMOSD and MS.

Conclusion

The novel CNN model we proposed could automatically differentiate the rare NMOSD from MS, especially, our model showed better performance than traditional3D CNN models. It indicated that our 3D compressed CNN models are applicable in handling diseases with small-scale datasets and possess overlapping and scattered lesions.
Experimental Acute Pancreatitis Models: History, Current Status, and Role in Translational Research
Authors: Yang Xinmin , Yao Linbo , Fu Xianghui , Mukherjee Rajarshi , Xia Qing , Jakubowska Monika A. , Ferdek Pawel E. , Huang Wei
Published: December 2020

Acute pancreatitis is a potentially severe inflammatory disease that may be associated with a substantial morbidity and mortality. Currently there is no specific treatment for the disease, which indicates an ongoing demand for research into its pathogenesis and development of new therapeutic strategies. Due to the unpredictable course of acute pancreatitis and relatively concealed anatomical site in the retro-peritoneum, research on the human pancreas remains challenging. As a result, for over the last 100 years studies on the pathogenesis of this disease have heavily relied on animal models. This review aims to summarize different animal models of acute pancreatitis from the past to present and discuss their main characteristics and applications. It identifies key studies that have enhanced our current understanding of the pathogenesis of acute pancreatitis and highlights the instrumental role of animal models in translational research for developing novel therapies.
The Summer Is Coming: nocte and timeless Genes Are Influenced by Temperature Cycles and May Affect Aedes aegypti Locomotor Activity
Authors: Teles-de-Freitas Rayane , Rivas Gustavo B. S. , Peixoto Alexandre A. , Bruno Rafaela Vieira
Published: December 2020

Mosquitoes exhibit activity rhythms, crucial for the transmission of pathogens, under the control of a circadian clock.Aedes aegyptiis one of the world’s leading vectors. For decades, several studies have linked the rise in ambient temperature with the increase in their activity. Here, we identify candidate genes whose expression is influenced by temperature cycles and may affectAedeslocomotor activity. We observed thattimelesscompletely lost its rhythmic expression in light/dark, with out-of-phase temperature cycles, and by RNAi mediated knockdown ofnocte, an important gene forDrosophilacircadian synchronization by temperature cycles. Thus,timelessandnocteare important genes for synchronization by temperature cycles inAedes aegypti. To reinforce our findings, we simulated in the laboratory the gradual temperature fluctuations that were as close as possible to daily temperature variations in Brazil. We observed that the activity and the expression of the molecular circadian clock ofAe. aegyptidiffers significantly from that of mosquitoes subjected to constant or rectangular abrupt changes in temperature. We suggest that for understanding the circadian behavior ofAedeswith possible implications for intervention strategies, the seminatural paradigm needs to replace the traditional laboratory study.
Role of the α7 Nicotinic Acetylcholine Receptor in the Pathophysiology of Atherosclerosis
Authors: Vieira-Alves Ildernandes , Coimbra-Campos Leda M. C. , Sancho Maria , da Silva Rafaela Fernandes , Cortes Steyner F. , Lemos Virgínia Soares
Published: December 2020

Atherosclerosis constitutes a major risk factor for cardiovascular diseases, the leading cause of morbidity and mortality worldwide. This slowly progressing, chronic inflammatory disorder of large- and medium-sized arteries involves complex recruitment of immune cells, lipid accumulation, and vascular structural remodeling. The α7 nicotinic acetylcholine receptor (α7nAChR) is expressed in several cell types involved in the genesis and progression of atherosclerosis, including macrophages, dendritic cells, T and B cells, vascular endothelial and smooth muscle cells (VSMCs). Recently, the α7nAChR has been described as an essential regulator of inflammation as this receptor mediates the inhibition of cytokine synthesis through the cholinergic anti-inflammatory pathway, a mechanism involved in the attenuation of atherosclerotic disease. Aside from the neuronal cholinergic control of inflammation, the non-neuronal cholinergic system similarly regulates the immune function. Acetylcholine released from T cells acts in an autocrine/paracrine fashion at the α7nAChR of various immune cells to modulate immune function. This mechanism additionally has potential implications in reducing atherosclerotic plaque formation. In contrast, the activation of α7nAChR is linked to the induction of angiogenesis and VSMC proliferation, which may contribute to the progression of atherosclerosis. Therefore, both atheroprotective and pro-atherogenic roles are attributed to the stimulation of α7nAChRs, and their role in the genesis and progression of atheromatous plaque is still under debate. This minireview highlights the current knowledge on the involvement of the α7nAChR in the pathophysiology of atherosclerosis.
Development of Neonectria punicea Pathogenic Symptoms in Juvenile Fraxinus excelsior Trees
Authors: Karadžić Dragan , Stanivuković Zoran , Milanović Slobodan , Sikora Katarzyna , Radulović Zlatan , Račko Vladimír , Kardošová Monika , Ďurkovič Jaroslav , Milenković Ivan
Published: December 2020

When monitoring the state of health of Fraxinus excelsior trees, unusual symptoms were discovered within a F. excelsior plantation in Bosnia and Herzegovina. These symptoms included the appearance of necrosis and cankers in the basal parts of the trees, followed by the formation of fruiting bodies, however, none of these symptoms were found in the crowns. After sampling and isolation of the necrotic parts from the stem base, pathogen Neonectria punicea was isolated and identified from the characteristics of pure cultures, morphology of the fruiting bodies, and from multilocus sequencing. In field conditions, juvenile F. excelsior trees were inoculated with two N. punicea isolates obtained from the necrotic tissues of both juvenile F. excelsior and mature Fagus sylvatica trees. In both isolates, 12 months post inoculation, the lengths and widths of the necroses were significantly larger compared to the control. Necroses of significantly larger lengths, widths and surfaces were found again in both tested isolates 24 months post inoculation. In the case of the F. excelsior isolate, the lengths of the necroses at both the stem base and at breast height increased by 1.6 times, whereas the F. sylvatica isolate increased in size by up to 1.7 and 1.8 times, respectively. Trees inoculated without a previous bark wound showed no symptoms, similar to the control trees. Scanning electron microscopy and X-ray micro-computed tomography imaging revealed that N. punicea hyphae penetrated from the cankers to the woody outermost annual growth ring and that hyphae were present mostly in the large earlywood vessels and rarely in the axial parenchyma cells. Hyphae also spread radially through the pits in vessels. The infected trees responded with the formation of tyloses in the vessels to prevent a rapid fungal spread through the axial vascular transport pathway. The ability of N. punicea to cause necroses in juvenile ash trees was demonstrated for the first time during this study. It poses a serious threat to planted forests and natural regenerations of F. excelsior especially if F. sylvatica is considered as a possible inoculum reservoir for future infections. This pathogen should be integrated within future ash resistance or breeding programs.
Dynamic Changes of the Anthocyanin Biosynthesis Mechanism During the Development of Heading Chinese Cabbage (Brassica rapa L.) and Arabidopsis Under the Control of BrMYB2
Authors: He Qiong , Lu Qianqian , He Yuting , Wang Yaxiu , Zhang Ninan , Zhao Wenbin , Zhang Lugang
Published: December 2020

Chinese cabbage is an important vegetable mainly planted in Asian countries, and mining the molecular mechanism responsible for purple coloration in Brassica crops is fast becoming a research hotspot. In particular, the anthocyanin accumulation characteristic of purple heading Chinese cabbage, along with the plant’s growth and head developing, is still largely unknown. To elucidate the dynamic anthocyanin biosynthesis mechanism of Chinese cabbage during its development processes, here we investigated the expression profiles of 86 anthocyanin biosynthesis genes and corresponding anthocyanin accumulation characteristics of plants as they grew and their heads developed, between purple heading Chinese cabbage 11S91 and its breeding parents. Anthocyanin accumulation of 11S91 increased from the early head formation period onward, whereas the purple trait donor 95T2-5 constantly accumulated anthocyanin throughout its whole plant development. Increasing expression levels of BrMYB2 and BrTT8 together with the downregulation of BrMYBL2.1, BrMYBL2.2, and BrLBD39.1 occurred in both 11S91 and 95T2-5 plants during their growth, accompanied by the significantly continuous upregulation of a phenylpropanoid metabolic gene, BrPAL3.1; a series of early biosynthesis genes, such as BrCHSs, BrCHIs, BrF3Hs, and BrF3’H; as well as some key late biosynthesis genes, such as BrDFR1, BrANS1, BrUF3GT2, BrUF5GT, Br5MAT, and Brp-Cout; in addition to the transport genes BrGST1 and BrGST2. Dynamic expression profiles of these upregulated genes correlated well with the total anthocyanin contents during the processes of plant growth and leaf head development, and results supported by similar evidence for structural genes were also found in the BrMYB2 transgenic Arabidopsis. After intersubspecific hybridization breeding, the purple interior heading leaves of 11S91 inherited the partial purple phenotypes from 95T2-5 while the phenotypes of seedlings and heads were mainly acquired from white 94S17; comparatively in expression patterns of investigated anthocyanin biosynthesis genes, cotyledons of 11S91 might inherit the majority of genetic information from the white type parent, whereas the growth seedlings and developing heading tissues of 11S91 featured expression patterns of these genes more similar to 95T2-5. This comprehensive set of results provides new evidence for a better understanding of the anthocyanin biosynthesis mechanism and future breeding of new purple Brassica vegetables.
The Economic Impact of Exchanging Breeding Material: Assessing Winter Wheat Production in Germany
Authors: Lüttringhaus Sophia , Gornott Christoph , Wittkop Benjamin , Noleppa Steffen , Lotze-Campen Hermann
Published: December 2020

Climate change impacts imply that the stabilization and improvement of agricultural production systems using technological innovations has become vital. Improvements in plant breeding are integral to such innovations. In the context of German crop breeding programs, the economic impact of exchanging genetic material has yet to be determined. To this end, we analyze in this impact assessment the economic effects on German winter wheat production that are attributable to exchanging parental material amongst breeders in the breeding process. This exchange is supported by the breeders’ exemption, which is an integral part of the German plant variety protection legislation. It ensures that breeders can freely use licensed varieties created by other breeders for their own breeding activities and aims to speed up the development of improved varieties. For our analysis, we created a unique data set that combines variety-specific grain yield, adoption, and pedigree information of 133 winter wheat varieties. We determined the parental pedigree of each variety to see if a variety was created by interbreeding varieties that are internal or external to its specific breeder. Our study is the first that analyzes the economic impact of exchanging genetic material in German breeding programs. We found that more than 90 % of the tested varieties were bred with exchanged parental material, whereby the majority had two external parents. Also, these varieties were planted on an 8.5 times larger area than the varieties that were bred with two internal parents. Due to lower adoption, these only contributed 11 % to the overall winter wheat production in Germany, even though they yielded more. We used an economic surplus model to measure the benefits of exchanging parental breeding material on German winter wheat production. This resulted in an overall estimated economic surplus of 19.2 to 22.0 billion EUR from production year 1972 to 2018. This implies tremendous returns to using the breeder’s exemption, which, from an economic perspective, is almost cost-free for the breeder. We conclude that the exchange of breeding material contributes to improving Germany’s agricultural production and fosters the development of climate-resilient production systems and global food security.
Empty Pericarp24 and Empty Pericarp25 Are Required for the Splicing of Mitochondrial Introns, Complex I Assembly, and Seed Development in Maize
Authors: Xiu Zhihui , Peng Ling , Wang Yong , Yang Huanhuan , Sun Feng , Wang Xiaomin , Cao Shi-Kai , Jiang Ruicheng , Wang Le , Chen Bao-Yin , Tan Bao-Cai
Published: December 2020

RNA splicing is an essential post-transcriptional regulation in plant mitochondria and chloroplasts. As the mechanism of RNA splicing remains obscure, identification and functional elucidation of new splicing factors are necessary. Through a characterization of two maize mutants, we clonedEmpty pericarp 24(Emp24) andEmpty pericarp 25(Emp25). BothEmp24andEmp25encode mitochondrion-targeted P-type PPR proteins. EMP24 is required for the splicing ofnad4introns 1 and 3, which was reported (Ren Z. et al., 2019), and EMP25 functions in the splicing ofnad5introns 1, 2, and 3. Absence of either Nad4 or Nad5 proteins blocks the assembly of mitochondrial complex I, resulting in the formation of a sub-sized complex I of similar size in both mutants. Mass spectrometry identification revealed that the subcomplexes in both mutants lack an identical set of proteins of complex I. These results indicate that EMP24 and EMP25 function in the splicing ofnad4andnad5introns, respectively, and are essential to maize kernel development. The identification of the subcomplexes provides genetic and molecular insights into the modular complex I assembly pathway in maize.
Reprogramming of Tomato Leaf Metabolome by the Activity of Heat Stress Transcription Factor HsfB1
Authors: Paupière Marine Josephine , Tikunov Yury , Schleiff Enrico , Bovy Arnaud , Fragkostefanakis Sotirios
Published: December 2020

Plants respond to high temperatures with global changes of the transcriptome, proteome, and metabolome. Heat stress transcription factors (Hsfs) are the core regulators of transcriptome responses as they control the reprogramming of expression of hundreds of genes. The thermotolerance-related function of Hsfs is mainly based on the regulation of many heat shock proteins (HSPs). Instead, the Hsf-dependent reprogramming of metabolic pathways and their contribution to thermotolerance are not well described. In tomato (Solanum lycopersicum), manipulation of HsfB1, either by suppression or overexpression (OE) leads to enhanced thermotolerance and coincides with distinct profile of metabolic routes based on a metabolome profiling of wild-type (WT) and HsfB1 transgenic plants. Leaves of HsfB1 knock-down plants show an accumulation of metabolites with a positive effect on thermotolerance such as the sugars sucrose and glucose and the polyamine putrescine. OE of HsfB1 leads to the accumulation of products of the phenylpropanoid and flavonoid pathways, including several caffeoyl quinic acid isomers. The latter is due to the enhanced transcription of genes coding key enzymes in both pathways, in some cases in both non-stressed and stressed plants. Our results show that beyond the control of the expression of Hsfs and HSPs, HsfB1 has a wider activity range by regulating important metabolic pathways providing an important link between stress response and physiological tomato development.
Psychological Factors Influencing Pro-environmental Behavior in Developing Countries: Evidence From Colombian and Nicaraguan Students
Authors: Díaz Manuel Francisco , Charry Andrés , Sellitti Stefania , Ruzzante Matteo , Enciso Karen , Burkart Stefan
Published: December 2020

Identifying the determinants of human behavior is useful to adjust interventions and lead the civil society toward a stronger commitment to climate change (CC) mitigation and adaptation objectives, achieving greater support for successfully implementing environmental policies. Existing research has largely focused on case studies of pro-environmental behaviors (PEBs) in developed economies but there is very little evidence for developing countries. This study provides estimations of the effect of internal factors, such as sociodemographic variables, and four psychological dimensions (CC knowledge, environmental attitudes, self-efficacy, and trust in sources of environmental information) on PEBs. Data were obtained through a survey applied with future decision makers – university students – from Colombia (n = 4,769) and Nicaragua (n = 2,354). Indices were generated for PEBs and the psychological dimensions using z-scores and Principal Component Analysis (PCA). Partial correlations were evaluated through the Ordinary Least Squares (OLS) method. Our results suggest that, in order to reach the planned emission reduction targets, policy approaches should more strongly focus on educating and motivating citizens and prepare them for contributing to the environmental cause, as well as provide individual solutions to combat CC, rather than providing only information on its causes and consequences.
The Role of Personal and Political Values in Predicting Environmental Attitudes and Pro-environmental Behavior in Kazakhstan
Authors: Agissova Fatikha , Sautkina Elena
Published: December 2020

Although it is widely accepted that personal values of Self-Transcendence are a positive predictor of environmentalism, and Self-Enhancement values are a negative one, these results are not conclusive for all cultural contexts. Regarding political ideologies, research concludes that liberals tend to be more concerned about the environment than conservatives. However, this two-dimensional take on political ideologies does not grasp the diversity of political views, which could be achieved by focusing on political values. In this research, we studied the role of personal and political values in predicting environmental attitudes and behavior in Kazakhstan, a developing country in Central Asia. Using an online survey (n = 305), we found that Security was a strong predictor of both environmental concern and New Environmental Paradigm (NEP), overshadowing the effect of traditionally accepted value dimensions of Self-Transcendence and Self-Enhancement. While Self-Direction positively predicted environmental concern, Universalism and Benevolence were positive predictors of NEP. Among political values, Civil Liberties predicted NEP positively, and had no significant effect on environmental concern, while Free Enterprise predicted environmental concern negatively, and had no significant effect on NEP. Environmental concern was a strong predictor of all pro-environmental behaviors included in the study (littering, recycling, environmental citizenship, and community action), fully mediating the effect of NEP. Based on personal and political values, three profiles of Kazakhstanis who engaged differently in pro-environmental behavior were identified.
Convince Yourself to Do the Right Thing: The Effects of Provided Versus Self-Generated Arguments on Rule Compliance and Perceived Importance of Socially Desirable Behavior
Authors: Lemmen Nieke , Keizer Kees , Bouman Thijs , Steg Linda
Published: December 2020

One way to enhance rule compliance is to provide people with arguments explaining why the desired behavior is important. We argue that there might be another, potentially more effective way to enhance rule compliance: ask people to generate arguments in favor of the rule themselves, which can trigger a process of self-persuasion. We compared the effects of providing arguments, asking respondents to generate arguments themselves, and a combination of both approaches on rule compliance and the perceived importance of the rule. A field experiment revealed that rule compliance was higher in all experimental conditions compared to a control condition, with the highest level of rule compliance in the conditions that either presented the arguments or asked people to generate arguments themselves. Yet the rule was only evaluated as more important compared to the control condition, when people generated arguments themselves. This study suggests that rule compliance and perceived importance of this rule can be enhanced by easy low-cost interventions.
Factor Structure and Measurement Invariance Across Gender of the Beck Depression Inventory-II in Adolescent Psychiatric Patients
Authors: Keller Ferdinand , Kirschbaum-Lesch Inken , Straub Joana
Published: December 2020

The revised version of the Beck Depression Inventory (BDI-II) is one of the most frequently applied questionnaires not only in adults, but also in adolescents. To date, attempts to identify a replicable factor structure of the BDI-II have mainly been undertaken in adult populations. Moreover, most of the studies which included minors and were split by gender lacked confirmatory factor analyses and were generally conducted in healthy adolescents. The present study therefore aimed to determine the goodness of fit of various factor models proposed in the literature in an adolescent clinical sample, to evaluate alternative solutions for the factor structure and to explore potential gender differences in factor loadings. The focus was on testing bifactor models and subsequently on calculating bifactor statistical indices to help clarify whether a uni- or a multidimensional construct is more appropriate, and on testing the best-fitting factor model for measurement invariance according to gender. The sample comprised 835 adolescent girls and boys aged 13–18 years in out- and inpatient setting. Several factor models proposed in the literature provided a good fit when applied to the adolescent clinical sample, and differences in goodness of fit were small. Exploratory factor analyses were used to develop and test a bifactor model that consisted of a general factor and two specific factors, termed cognitive and somatic. The bifactor model confirmed the existence of a strong general factor on which all items load, and the bifactor statistical indices suggest that the BDI-II should be seen as a unidimensional scale. Concerning measurement invariance across gender, there were differences in loadings on item 21 (Loss of interest in sex) on the general factor and on items 1 (Sadness), 4 (Loss of pleasure), and 9 (Suicidal Thoughts) on the specific factors. Thus, partial measurement invariance can be assumed and differences are negligible. It can be concluded that the total score of the BDI-II can be used to measure depression severity in adolescent clinical samples.
Mental Health Determinants Among a Psychiatric Outpatient Sample of Vietnamese Migrants in Germany
Authors: Wolf Simon , Hahn Eric , Wingenfeld Katja , Nguyen Main Huong , von Poser Anita , Nguyen Thi Hoa , Hanewald Bernd , Böge Kerem , Bajbouj Malek , Dettling Michael , Nguyen Van Tuan , Ta Thi Minh Tam
Published: December 2020

Background: Mental health risk-factors for Asian migrants have been studied almost exclusively in the US, Canada, and Australia but not in European countries. Therefore, we aimed to identify sociodemographic, clinical, and migration-surrounding factors associated with experienced mental distress among Vietnamese migrants in Germany.

Method: 305 Vietnamese migrants utilizing Germany's first Vietnamese psychiatric outpatient clinic filled out at admission the Brief-Symptom-Inventory 18 (BSI-18) as well as a questionnaire on 22 potential mental health determinants. Using a multiple linear regression model, we identified those sociodemographic, clinical, and migration-surrounding factors that were significantly related to the Global Severity Index (GSI) of the BSI-18.

Results: The factors unemployment (B = −6.32, p = 0.014), financial problems (B = −10.71, p < 0.001), no or only little religious involvement (B = −3.23, p = 0.002), no psychiatric precontact (B = −7.35, p = 0.004), previous migration experiences (B = 8.76, p = 0.002), and perceived discrimination (B = 6.58, p = 0.011) were found to significantly increase the level of mental distress according to the BSI-GSI.

Conclusion: Based on these results, we were able to construct a mental health risk-profile for Vietnamese migrants in Germany, which aims to detect candidates for psychiatric problems earlier and supply them with customized prevention and therapy options.
What Did We Learn From Plant Growth-Promoting Rhizobacteria (PGPR)-Grass Associations Studies Through Proteomic and Metabolomic Approaches?
Authors: Alberton Dayane , Valdameri Glaucio , Moure Vivian Rotuno , Monteiro Rose Adele , Pedrosa Fabio de Oliveira , Müller-Santos Marcelo , de Souza Emanuel Maltempi
Published: December 2020

Plant growth stimulation by microorganisms that interact in a mutually beneficial manner remains poorly understood. Understanding the nature of plant-bacteria interactions may open new routes for plant productivity enhancement, especially cereal crops consumed by humans. Proteomic and metabolomic analyses are particularly useful for elucidating these mechanisms. A complete depiction of these mechanisms will prompt researchers to develop more efficient plant-bacteria associations. The success of microorganisms as biofertilizers may replace the current massive use of chemical fertilizers, mitigating many environmental and economic issues. In this review, we discuss the recent advances and current state of the art in proteomics and metabolomics studies involving grass-bacteria associations. We also discuss essential subjects involved in the bacterial plant-growth promotion, such, nitrogen fixation, plant stress, defense responses, and siderophore production.
Key Considerations for the Use of Seaweed to Reduce Enteric Methane Emissions From Cattle
Authors: Vijn Sandra , Compart Devan Paulus , Dutta Nikki , Foukis Athanasios , Hess Matthias , Hristov Alexander N. , Kalscheur Kenneth F. , Kebreab Ermias , Nuzhdin Sergey V. , Price Nichole N. , Sun Yan , Tricarico Juan M. , Turzillo Adele , Weisbjerg Martin R. , Yarish Charles , Kurt Timothy D.
Published: December 2020

Enteric methane emissions are the single largest source of direct greenhouse gas emissions (GHG) in beef and dairy value chains and a substantial contributor to anthropogenic methane emissions globally. In late 2019, the World Wildlife Fund (WWF), the Advanced Research Projects Agency-Energy (ARPA-E) and the Foundation for Food and Agriculture Research (FFAR) convened approximately 50 stakeholders representing research and production of seaweeds, animal feeds, dairy cattle, and beef and dairy foods to discuss challenges and opportunities associated with the use of seaweed-based ingredients to reduce enteric methane emissions. This Perspective article describes the considerations identified by the workshop participants and suggests next steps for the further development and evaluation of seaweed-based feed ingredients as enteric methane mitigants. Although numerous compounds derived from sources other than seaweed have been identified as having enteric methane mitigation potential, these mitigants are outside the scope of this article.
Resistance to Fusarium Head Blight, Kernel Damage, and Concentration of Fusarium Mycotoxins in Grain of Winter Triticale (x Triticosecale Wittmack) Lines
Authors: Góral Tomasz , Wiśniewska Halina , Ochodzki Piotr , Twardawska Adriana , Walentyn-Góral Dorota
Published: December 2020

Fusarium head blight (FHB) can cause contamination of cereal grain with mycotoxins. Triticale is also infected with FHB; however, it is more resistant than wheat to head infection. The aim of this study was to identify triticale lines that combine low head infection with low toxin contamination. Resistance to FHB of 15 winter triticale and three winter wheat lines was evaluated over a three-year experiment established in two locations. At the anthesis stage, heads were inoculated with Fusarium culmorum isolates. The FHB index was scored and the percentage of Fusarium-damaged kernels (FDKs) assessed. The grain was analysed for type B trichothecenes (deoxynivalenol and derivatives, nivalenol) and zearalenone content. The average FHB index was 10.7%. The proportion of FDK was 18.1% (weight) and 21.6% (number). An average content of deoxynivalenol amounted to 7.258 mg/kg and nivalenol to 5.267 mg/kg. In total, it was 12.788 mg/kg of type B trichothecenes. The zearalenone content in the grain was 0.805 mg/kg. Relationships between FHB index, FDK, and mycotoxin contents were statistically significant for triticale lines; however, they were stronger for FDK versus mycotoxins. Triticale lines combing all types of FHB resistance were found, however the most resistant ones were less resistant that wheat lines with the Fhb1 gene.
Transmissible Viral Proventriculitis Caused by Chicken Proventricular Necrosis Virus Displaying Serological Cross-Reactivity with IBDV
Authors: Śmiałek Marcin , Gesek Michał , Dziewulska Daria , Niczyporuk Jowita Samanta , Koncicki Andrzej
Published: December 2020

Transmissible viral proventriculitis (TVP) of chickens is manifested in decreased body weight gains, poor feed conversion and weight diversity. Although TVP etiology has not been defined, a Birnaviridae family member, named chicken proventricular necrosis virus (CPNV) is considered as a potential factor of a disease. This study was undertaken in order to reproduce TVP and to evaluate its etiology. Broiler chickens of the TVP-infected group were inoculated with TVP positive proventriculi homogenate on the 24th day of life. Samples were collected, on infection day and 14 days post-infection (dpi). The 14 dpi anatomo- and histopathological evaluation, revealed that we have succeeded to reproduce TVP. TVP-infected birds gained 30.38% less body weight. In the TVP-infected group a seroconversion against picornaviruses, fowl adenoviruses (FAdV) and infectious bursal disease viruses (IBDV) was recorded with an ELISA test. Using RT-PCR and PCR, CPNV was detected in proventriculi and FAdV in spleens and livers of infected birds, 14 dpi. Our study supports that CPNV is involved in the development of TVP. We did not record the presence of IBDV in TVP or control birds, despite our recording of a seroconversion against IBDV in TVP infected birds. CPNV and IBDV belong to the same family, which allows us to assume serological cross-reactivity between them. The role of FAdV needs further evaluation.
Feasibility of a Helium Closed-Cycle Gas Turbine for UAV Propulsion
Authors: Osigwe Emmanuel O. , Gad-Briggs Arnold , Nikolaidis Theoklis
Published: December 2020

When selecting a design for an unmanned aerial vehicle, the choice of the propulsion system is vital in terms of mission requirements, sustainability, usability, noise, controllability, reliability and technology readiness level (TRL). This study analyses the various propulsion systems used in unmanned aerial vehicles (UAVs), paying particular focus on the closed-cycle propulsion systems. The study also investigates the feasibility of using helium closed-cycle gas turbines for UAV propulsion, highlighting the merits and demerits of helium closed-cycle gas turbines. Some of the advantages mentioned include high payload, low noise and high altitude mission ability; while the major drawbacks include a heat sink, nuclear hazard radiation and the shield weight. A preliminary assessment of the cycle showed that a pressure ratio of 4, turbine entry temperature (TET) of 800 °C and mass flow of 50 kg/s could be used to achieve a lightweight helium closed-cycle gas turbine design for UAV mission considering component design constraints.
Digital Twins in Product Lifecycle for Sustainability in Manufacturing and Maintenance
Authors: Rojek Izabela , Mikołajewski Dariusz , Dostatni Ewa
Published: December 2020

A “digital twin” is a dynamic, digital replica of a technical object (e.g., a physical system, device, machine or production process) or a living organism. Using this type of solution has become an integral part of Industry 4.0, offering businesses tangible benefits, in addition to being particularly effective within the context of sustainable production and maintenance. The purpose of this paper is to present the results of research on the development of digital twins of technical objects, which involved data acquisition and their conversion into knowledge, the use of physical models to simulate tasks and processes, and the use of simulation models to improve the physical tasks and processes. In addition, monitoring processes and process parameters allow for the continued improvement of existing processes as regards intelligent eco-designing and planning and monitoring production processes while taking into account sustainable production and maintenance.
A New Coking Coal Charging Method for 6 m Top-Charged Coke Oven: System Design and Experiment
Authors: Zhang Ze , Zhang Shuting
Published: December 2020

Coking with low moisture coal is an important link of energy conservation and coke quality improvement in the coking industry. Aiming at the problems of dust emission and bad accumulation in the coking chamber during coal charging, a new system of coking coal charged into 6 m top-charge coke oven was studied and designed, in which a cylinder with telescopic and high temperature resistance was used to fill the coking chamber with a dense phase continuous flow. The coal transport characteristics, dust emission, and accumulation characteristics were studied through the actual operation of the equipment. The results found that the matching of spiral feeding speed and cylinder lifting speed had an important influence on the transport characteristics, when moisture was 5%, and the control dense phase transport conditions were—cylinder lifting speed of 0.02 m/s and spiral feeding speed control range of [0.31 m/s, 0.50 m/s]. The new device was found to reduce dust emission by 90% per square meter, compared to the traditional. The influence of controllable factors on the accumulation characteristics of coal was studied, and the essential conditions for optimal repose angle and bulk density were obtained through an orthogonal test, the prediction model of accumulation characteristics was established.
Model Validation and Scenario Selection for Virtual-Based Homologation of Automated Vehicles
Authors: Riedmaier Stefan , Schneider Daniel , Watzenig Daniel , Diermeyer Frank , Schick Bernhard
Published: December 2020

Due to the rapid progress in the development of automated vehicles over the last decade, their market entry is getting closer. One of the remaining challenges is the safety assessment and type approval of automated vehicles, as conventional testing in the real world would involve an unmanageable mileage. Scenario-based testing using simulation is a promising candidate for overcoming this approval trap. Although the research community has recognized the importance of safeguarding in recent years, the quality of simulation models is rarely taken into account. Without investigating the errors and uncertainties of models, virtual statements about vehicle safety are meaningless. This paper describes a whole process combining model validation and safety assessment. It is demonstrated by means of an actual type-approval regulation that deals with the safety assessment of lane-keeping systems. Based on a thorough analysis of the current state-of-the-art, this paper introduces two approaches for selecting test scenarios. While the model validation scenarios are planned from scratch and focus on scenario coverage, the type-approval scenarios are extracted from measurement data based on a data-driven pipeline. The deviations between lane-keeping behavior in the real and virtual world are quantified using a statistical validation metric. They are then modeled using a regression technique and inferred from the validation experiments to the unseen virtual type-approval scenarios. Finally, this paper examines safety-critical lane crossings, taking into account the modeling errors. It demonstrates the potential of the virtual-based safeguarding process using exemplary simulations and real driving tests.
High-Speed Autonomous Robotic Assembly Using In-Hand Manipulation and Re-Grasping
Authors: Kang Taewoong , Yi Jae-Bong , Song Dongwoon , Yi Seung-Joon
Published: December 2020

This paper presents an autonomous robotic assembly system for Soma cube blocks, which, after observing the individual blocks and their assembled shape, quickly plans and executes the assembly motion sequence that picks up each block and incrementally build the target shape. A multi stage planner is used to find the suitable assembly solutions, assembly sequences and grip sequences considering various constraints, and re-grasping is used when the block target pose is not directly realizable or the block pose is ambiguous. The suggested system is implemented for a commercial UR5e robotic arm and a novel two degrees of freedom (DOF) gripper capable of in-hand manipulation, which further speeds up the manipulation speed. It was experimentally validated through a public competitive demonstration, where the suggested system completed all assembly tasks reliably with outstanding performance.
First Report of Hepatitis E Virus in Shellfish in Southeast Italy
Authors: La Bella Gianfranco , Basanisi Maria Grazia , Nobili Gaia , Terio Valentina , Suffredini Elisabetta , La Salandra Giovanna
Published: December 2020

Hepatitis E virus (HEV) represents one of the principal causative agents of hepatitis globally. Among the five HEV genotypes affecting humans, genotypes 3 and 4 are zoonotic and are the main source of hepatitis E in developed countries. HEV has been detected in several foods. The present work investigated the presence of this virus in shellfish sold at retail in the Apulia region of Italy. The presence of HEV RNA was assessed by real-time RT-PCR in 225 shellfish samples collected during 2018. Overall, two (0.89%) of these samples tested positive for HEV RNA. To our knowledge, this is the first notification of the detection of HEV in mussels sold at retail in the Apulia region. These data highlight the potential role of shellfish as a vehicle for the transmission of viral pathogens.
Gasification Char and Used Foundry Sand as Alternative Fillers to Graphene Nanoplatelets for Electrically Conductive Mortars with and without Virgin/Recycled Carbon Fibres
Authors: Mobili Alessandra , Giosuè Chiara , Bellezze Tiziano , Revel Gian Marco , Tittarelli Francesca
Published: December 2020

Structural health monitoring to assess the safety, durability and performance of structures can be performed by non-destructive methods such as the measurement of impedance in self-sensing cement-based elements. Cement-based materials, like mortars and concretes, generally have high electrical resistivity but the addition of carbon-based fillers and fibres decreases their electrical resistivity and thus enhances their self-sensing capabilities. In this study, two waste carbon-based fillers, namely, used foundry sand and gasification char were compared to commercial graphene nanoplatelets and used to produce self-sensing cement mortars, both with and without recycled or virgin carbon fibres. The mortars were tested in terms of their mechanical and electrical properties as well as their propensity to capillary water absorption. The results demonstrate that gasification char alone is the best carbonaceous waste for decreasing the electrical resistivity (−42%) and water absorption (−17%) of mortars, while their compressive strength remains unaltered. Moreover, although there is a slight reduction in compressive strength and an increase in water suction when gasification char is coupled with fibres, the combination of fillers and fibres has a synergistic effect in decreasing mortars’ electrical resistivity, especially when recycled carbon fibres are used (−80%).
Boundary Deformation Measurement by Mesh-Based Digital Image Correlation Method
Authors: Wang Lu , Liu Guangyan , Xing Tongzhen , Zhu Haibin , Ma Shaopeng
Published: December 2020

Digital image correlation (DIC) is a popular photomechanics method for deformation measurement. The conventional subset-based DIC method obtains the displacement vectors at the subset centers, but cannot calculate the deformation on the specimen edges, which may contain very useful information because specimens usually have greater deformation on edges, especially on curved edges due to stress concentration. In this study, the capability of a mesh-based DIC method using 8-node quadrilateral elements (Q8-mesh-DIC) in boundary deformation measurement was investigated and highlighted for specimens with non-uniform deformation. The results were compared with those obtained by some conventional subset-based DIC methods, and the accuracy of the boundary deformation measurement was verified through simulated and real experiments. The Q8-mesh-DIC appears to be more suitable for the boundary deformation measurement of non-uniform deformation fields.