bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of RecA inactivation and detoxification systems on the evolution of ciprofloxacin resistance in Escherichia coli
Authors: Diaz-Diaz S , Recacha E , García-Duque A , Docobo-Pérez F , Blázquez J , Pascual A , Rodríguez-Martínez J M
Published: November 2021

Background

Suppression of SOS response and overproduction of reactive oxygen species (ROS) through detoxification system suppression enhance the activity of fluoroquinolones.

Objectives

To evaluate the role of both systems in the evolution of resistance to ciprofloxacin in an isogenic model of Escherichia coli.

Methods

Single-gene deletion mutants of E. coli BW25113 (wild-type) (ΔrecA, ΔkatG, ΔkatE, ΔsodA, ΔsodB), double-gene (ΔrecA-ΔkatG, ΔrecA-ΔkatE, ΔrecA-ΔsodA, ΔrecA-ΔsodB, ΔkatG-ΔkatE, ΔsodB-ΔsodA) and triple-gene (ΔrecA-ΔkatG-ΔkatE) mutants were included. The response to sudden high ciprofloxacin pressure was evaluated by mutant prevention concentration (MPC). The gradual antimicrobial pressure response was evaluated through experimental evolution and antibiotic resistance assays.

Results

For E. coli BW25113 strain, ΔkatE, ΔsodB and ΔsodB/ΔsodA mutants, MPC values were 0.25 mg/L. The ΔkatG, ΔsodA, ΔkatG/katE and ΔrecA mutants showed 2-fold reductions (0.125 mg/L). The ΔkatG/ΔrecA, ΔkatE/ΔrecA, ΔsodA/ΔrecA, ΔsodB/ΔrecA and ΔkatG/ΔkatE/ΔrecA strains showed 4–8-fold reductions (0.03–0.06 mg/L) relative to the wild-type. Gradual antimicrobial pressure increased growth capacity for ΔsodA and ΔsodB and ΔsodB/ΔsodA mutants (no growth in 4 mg/L) compared with the wild-type (no growth in the range of 0.5–2 mg/L). Accordingly, increased growth was observed with the mutants ΔrecA/ΔkatG (no growth in 2 mg/L), ΔrecA/ΔkatE (no growth in 2 mg/L), ΔrecA/ΔsodA (no growth in 0.06 mg/L), ΔrecA/ΔsodB (no growth in 0.25 mg/L) and ΔrecA/ΔkatG/ΔkatE (no growth in 0.5 mg/L) compared with ΔrecA (no growth in the range of 0.002–0.015 mg/L).

Conclusions

After RecA inactivation, gradual exposure to ciprofloxacin reduces the evolution of resistance. After suppression of RecA and detoxification systems, sudden high exposure to ciprofloxacin reduces the evolution of resistance in E. coli.
The role of unprotected and privately protected areas for ocelot conservation: densities in Colombia and Brazil
Authors: Boron Valeria , Bardales Rocío , Hyde Matthew , Jaimes-Rodriguez Laura , Stasiukynas Diana , Barragan Jorge , Passos Viana Diego Francis , Payán Esteban
Published: November 2021

The ocelot (Leopardus pardalis) is a widely distributed, medium-sized felid in the Americas with declining population size. We estimated ocelot densities and home ranges in one agricultural area in the Magdalena River valley in Colombia, a private reserve and cattle ranch in the Colombian Llanos, and a private reserve in the Serra do Amolar in the Brazilian Pantanal. We used camera trapping (39–52 stations) and spatially explicit capture-recapture (SECR) models. Density estimates (individuals/100 km2) were 11.0 ± 2.7 (SE) in the Magdalena River valley; 13.2 ± 3.2 (SE) in the Llanos, and 10.3 ± 2.9 (SE) in the Serra do Amolar. Overall, despite an impact of agriculture and human disturbance, our results highlight the importance of unprotected areas and privately protected ranching areas for ocelot conservation. As agriculture continues to expand across the tropics causing habitat loss, and negatively affecting ocelot densities, we recommend land use planning and best agricultural practices to maintain natural habitats, thereby limiting human impacts on ocelot conservation.
Lysophosphatidic acid acyltransferases: a link with intracellular protein trafficking in Arabidopsis root cells?
Authors: Wattelet-Boyer Valérie , Le Guédard Marina , Dittrich-Domergue Franziska , Maneta-Peyret Lilly , Kriechbaumer Verena , Boutté Yohann , Bessoule Jean-Jacques , Moreau Patrick
Published: November 2021

Phosphatidic acid (PA) and lysophosphatidic acid acyltransferases (LPAATs) might be critical for the secretory pathway. Four extra-plastidial LPAATs (LPAAT2, 3, 4, and 5) were identified in Arabidopsis thaliana. These AtLPAATs display a specific enzymatic activity converting lysophosphatidic acid to PA and are located in the endomembrane system. We investigate a putative role for AtLPAATs 3, 4, and 5 in the secretory pathway of root cells through genetical (knockout mutants), biochemical (activity inhibitor, lipid analyses), and imaging (live and immuno-confocal microscopy) approaches. Treating a lpaat4;lpaat5 double mutant with the LPAAT inhibitor CI976 produced a significant decrease in primary root growth. The trafficking of the auxin transporter PIN2 was disturbed in this lpaat4;lpaat5 double mutant treated with CI976, whereas trafficking of H+-ATPases was unaffected. The lpaat4;lpaat5 double mutant is sensitive to salt stress, and the trafficking of the aquaporin PIP2;7 to the plasma membrane in the lpaat4;lpaat5 double mutant treated with CI976 was reduced. We measured the amounts of neo-synthesized PA in roots, and found a decrease in PA only in the lpaat4;lpaat5 double mutant treated with CI976, suggesting that the protein trafficking impairment was due to a critical PA concentration threshold.
Synthetic galaxy clusters and observations based on Dark Energy Survey Year 3 Data
Authors: Varga T N , Gruen D , Seitz S , MacCrann N , Sheldon E , Hartley W G , Amon A , Choi A , Palmese A , Zhang Y , Becker M R , McCullough J , Rozo E , Rykoff E S , To C , Grandis S , Bernstein G M , Dodelson S , Eckert K , Everett S , Gruendl R A , Harrison I , Herner K , Rollins R P , Sevilla-Noarbe I , Troxel M A , Yanny B , Zuntz J , Diehl H T , Jarvis M , Aguena M , Allam S , Annis J , Bertin E , Bhargava S , Brooks D , Carnero Rosell A , Carrasco Kind M , Carretero J , Costanzi M , da Costa L N , Pereira M E S , De Vicente J , Desai S , Dietrich J P , Ferrero I , Flaugher B , García-Bellido J , Gaztanaga E , Gerdes D W , Gschwend J , Gutierrez G , Hinton S R , Honscheid K , Jeltema T , Kuehn K , Kuropatkin N , Maia M A G , March M , Melchior P , Menanteau F , Miquel R , Morgan R , Myles J , Paz-Chinchón F , Plazas A A , Romer A K , Sanchez E , Scarpine V , Schubnell M , Serrano S , Smith M , Soares-Santos M , Suchyta E , Swanson M E C , Tarle G , Thomas D , Weller J ,   (DES Collaboration)
Published: November 2021

We develop a novel data-driven method for generating synthetic optical observations of galaxy clusters. In cluster weak lensing, the interplay between analysis choices and systematic effects related to source galaxy selection, shape measurement, and photometric redshift estimation can be best characterized in end-to-end tests going from mock observations to recovered cluster masses. To create such test scenarios, we measure and model the photometric properties of galaxy clusters and their sky environments from the Dark Energy Survey Year 3 (DES Y3) data in two bins of cluster richness $\lambda \in [30; 45)$, $\lambda \in [45; 60)$ and three bins in cluster redshift ($z\in [0.3; 0.35)$, $z\in [0.45; 0.5)$ and $z\in [0.6; 0.65)$. Using deep-field imaging data, we extrapolate galaxy populations beyond the limiting magnitude of DES Y3 and calculate the properties of cluster member galaxies via statistical background subtraction. We construct mock galaxy clusters as random draws from a distribution function, and render mock clusters and line-of-sight catalogues into synthetic images in the same format as actual survey observations. Synthetic galaxy clusters are generated from real observational data, and thus are independent from the assumptions inherent to cosmological simulations. The recipe can be straightforwardly modified to incorporate extra information, and correct for survey incompleteness. New realizations of synthetic clusters can be created at minimal cost, which will allow future analyses to generate the large number of images needed to characterize systematic uncertainties in cluster mass measurements.
The physics of galactic winds driven by cosmic rays I: Diffusion
Authors: Quataert Eliot , Thompson Todd A , Jiang (姜燕飞) Yan-Fei
Published: November 2021

The physics of Cosmic ray (CR) transport remains a key uncertainty in assessing whether CRs can produce galaxy-scale outflows consistent with observations. In this paper, we elucidate the physics of CR-driven galactic winds for CR transport dominated by diffusion. A companion paper considers CR streaming. We use analytic estimates validated by time-dependent spherically symmetric simulations to derive expressions for the mass-loss rate, momentum flux, and speed of CR-driven galactic winds, suitable for cosmological-scale or semi-analytic models of galaxy formation. For CR diffusion coefficients κ ≳ r0ci, where r0 is the base radius of the wind and ci is the isothermal gas sound speed, the asymptotic wind energy flux is comparable to that supplied to CRs, and the outflow rapidly accelerates to supersonic speeds. By contrast, for κ ≲ r0ci, CR-driven winds accelerate more slowly and lose most of their energy to gravity, a CR analogue of photon-tired stellar winds. Given CR diffusion coefficients estimated using Fermi gamma-ray observations of pion decay, we predict mass-loss rates in CR-driven galactic winds of the order of the star formation rate for dwarf and disc galaxies. The dwarf galaxy mass-loss rates are small compared to the mass-loadings needed to reconcile the stellar and dark matter halo mass functions. For nuclear starbursts (e.g. M82, Arp 220), CR diffusion and pion losses suppress the CR pressure in the galaxy and the strength of CR-driven winds. We discuss the implications of our results for interpreting observations of galactic winds and for the role of CRs in galaxy formation.
The physics of galactic winds driven by cosmic rays – II. Isothermal streaming solutions
Authors: Quataert Eliot , Jiang (姜燕飞) Yan-Fei , Thompson Todd A
Published: November 2021

We use analytical calculations and time-dependent spherically symmetric simulations to study the properties of isothermal galactic winds driven by cosmic rays (CRs) streaming at the Alfvén velocity. The simulations produce time-dependent flows permeated by strong shocks; we identify a new linear instability of sound waves that sources these shocks. The shocks substantially modify the wind dynamics, invalidating previous steady state models: the CR pressure pc has a staircase-like structure with dpc/dr ≃ 0 in most of the volume, and the time-averaged CR energetics are in many cases better approximated by pc ∝ ρ1/2, rather than the canonical pc ∝ ρ2/3. Accounting for this change in CR energetics, we analytically derive new expressions for the mass-loss rate, momentum flux, wind speed, and wind kinetic power in galactic winds driven by CR streaming. We show that streaming CRs are ineffective at directly driving cold gas out of galaxies, though CR-driven winds in hotter ISM phases may entrain cool gas. For the same physical conditions, diffusive CR transport (Paper I) yields mass-loss rates that are a few-100 times larger than streaming transport, and asymptotic wind powers that are a factor of ≃4 larger. We discuss the implications of our results for galactic wind theory and observations; strong shocks driven by CR-streaming-induced instabilities produce gas with a wide range of densities and temperatures, consistent with the multiphase nature of observed winds. We also quantify the applicability of the isothermal gas approximation for modelling streaming CRs and highlight the need for calculations with more realistic thermodynamics.
Sparse data inpainting for the recovery of Galactic-binary gravitational wave signals from gapped data
Authors: Blelly Aurore , Bobin Jérôme , Moutarde Hervé
Published: November 2021

The forthcoming space-based gravitational wave observatory LISA will open a new window for the measurement of Galactic binaries, which will deliver unprecedented information about these systems. However, the detection of Galactic binary gravitational wave signals is challenged by the presence of gaps in the data. Whether being planned or not, gapped data reduce our ability to detect faint signals and increase the risk of misdetection. Inspired by advances in signal processing, we introduce a non-parametric inpainting algorithm based on the sparse representation of the Galactic binary signal in the Fourier domain. In contrast to traditional inpainting approaches, noise statistics are known theoretically on ungapped measurements only. This calls for the joint recovery of both the ungapped noise and the Galactic binary signal. We thoroughly show that sparse inpainting yields an accurate estimation of the gravitational imprint of the Galactic binaries. Additionally, we highlight that the proposed algorithm produces a statistically consistent ungapped noise estimate. We further evaluate the performances of the proposed inpainting methods to recover the gravitational wave signal on a simple example involving verification Galactic binaries recently proposed in LISA data challenges.
Gravitational waves from spinning neutron stars as not-quite-standard sirens
Authors: Sieniawska Magdalena , Jones David Ian
Published: November 2021

As is well known, gravitational wave detections of coalescing binaries are standard sirens, allowing a measurement of source distance by gravitational wave means alone. In this paper we explore the analogue of this capability for continuous gravitational wave emission from individual spinning neutron stars, whose spin-down is driven purely by gravitational wave emission. We show that in this case, the distance measurement is always degenerate with one other parameter, which can be taken to be the moment of inertia of the star. We quantify the accuracy to which such degenerate measurements can be made. We also discuss the practical application of this method to scenarios where one or other of distance or moment of inertia is constrained, breaking this degeneracy and allowing a measurement of the remaining parameter. We consider a broad range of possible unknown parameters, as well as we present results for the aLIGO and Einstein Telescope sensitivities. Our results will be of use following the eventual detection of a neutron star spinning down through such gravitational wave emission.
Controlled RISC loading efficiency of miR168 defined by miRNA duplex structure adjusts ARGONAUTE1 homeostasis
Authors: Dalmadi Ágnes , Miloro Fabio , Bálint Jeannette , Várallyay Éva , Havelda Zoltán
Published: November 2021

Micro RNAs (miRNAs) are processed from precursor RNA molecules with precisely defined secondary stem-loop structures. ARGONAUTE1 (AGO1) is the main executor component of miRNA pathway and its expression is controlled via the auto-regulatory feedback loop activity of miR168 in plants. Previously we have shown that AGO1 loading of miR168 is strongly restricted leading to abundant cytoplasmic accumulation of AGO-unbound miR168. Here, we report, that intrinsic RNA secondary structure of MIR168a precursor not only defines the processing of miR168, but also precisely adjusts AGO1 loading efficiency determining the biologically active subset of miR168 pool. Our results show, that modification of miRNA duplex structure of MIR168a precursor fragment or expression from artificial precursors can alter the finely adjusted loading efficiency of miR168. In dcl1-9 mutant where, except for miR168, production of most miRNAs is severely reduced this mechanism ensures the elimination of unloaded AGO1 proteins via enhanced AGO1 loading of miR168. Based on this data, we propose a new competitive loading mechanism model for miR168 action: the miR168 surplus functions as a molecular buffer for controlled AGO1 loading continuously adjusting the amount of AGO1 protein in accordance with the changing size of the cellular miRNA pool.
Functional adaptation after kidney tissue removal in patients is associated with increased plasma atrial natriuretic peptide concentration
Authors: Azawi Nessn , Jensen Mia , Jensen Boye L , Gϕtze Jens P , Bistrup Claus , Lund Lars
Published: November 2021

Background

Following nephrectomy, the remaining kidney tissue adapts by an increase in glomerular filtration rate (GFR). In rats, hyperfiltration can be transferred by plasma. We examined whether natriuretic peptides, atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) increase in plasma proportionally with kidney mass reduction and, if so, whether the increase relates to an increase in GFR.

Methods

Patients (n = 54) undergoing partial or total unilateral nephrectomy at two Danish centres were followed for 1 year in an observational study. Glomerular filtration rate was measured before, and 3 and 12 months after surgery. Natriuretic propeptides (proANP and proBNP) and aldosterone were measured in plasma before and at 24 h, 5 days, 21 days, 3 months and 12 months. Cyclic guanosine monophosphate (cGMP) was determined in urine.

Results

There was no baseline difference in GFR between total and partial nephrectomy (90.1 mL/min/1.73 m2 ± 14.6 versus 82.9 ± 18; P = 0.16). Single-kidney GFR increased after 3 and 12 months (12.0 and 11.9 mL/min/1.73 m2, +23.3%). There was no change in measured GFR 3 and 12 months after partial nephrectomy. ProANP and proBNP increased 3-fold 24 h after surgery and returned to baseline after 5 days. The magnitude of acute proANP and proBNP increases did not relate to kidney mass removed. ProANP, not proBNP, increased 12 months after nephrectomy. Plasma aldosterone and urine cGMP did not change. Urine albumin/creatinine ratio increased transiently after partial nephrectomy. Blood pressure was similar between the groups.

Conclusion

ANP and BNP increase acutely in plasma with no relation to degree of kidney tissue ablation. After 1 year, only unilateral nephrectomy patients displayed increased plasma ANP, which could support adaptation.
Angiopoietin-2 predicts all-cause mortality in male but not female end-stage kidney disease patients on hemodialysis
Authors: Chu Chang , Chen Xin , Hasan Ahmed A , Szakallova Angelika , Krämer Bernhard K , Tepel Martin , Hocher Berthold
Published: November 2021

Background

Angiopoietin-2 (Ang-2) plays a pivotal role in pathological vascular remodeling and angiogenesis. Both vascular mechanisms are active in patients with end-stage renal disease (ESRD) and may contribute to the high mortality in these patients. The aim of this multicenter prospective cohort study was to investigate baseline serum Ang-2 concentrations in ESRD patients on hemodialysis (HD) for their ability to predict all-cause mortality.

Methods

We conducted a prospective cohort study in 340 stable HD patients from different chronic dialysis centers in Berlin, Germany. The primary endpoint was all-cause mortality during a 5-year follow-up period. Blood samples and clinical data were collected at baseline. Serum Ang-2 was measured with a validated enzyme-linked immunosorbent assay (Biomedica, Vienna, Austria).

Results

A total of 313 HD patients (206 men and 107 women) were finally included in the study. Receiver operating characteristic (ROC) analysis of Ang-2 concentrations yielded an area under the curve (AUC) of 0.65 (P < 0.0001) for predicting all-cause mortality in the entire study population and was used to determine the optimal cut-off (111.0 pmol/L) for all-cause mortality. Kaplan–Meier survival analysis indicated that male but not female end-stage kidney disease patients on HD with higher Ang-2 concentrations had a significantly lower survival (log-rank test, P < 0.0001 and P = 0.380 for male and female patients, respectively). Multivariable Cox regression analyses adjusted for age, comorbidity, smoking, dialysis vintage, serum creatinine, hemoglobin, C-reactive protein, serum albumin, intact parathyroid hormone (iPTH), low-density lipoprotein (LDL) and Kt/V likewise indicated that elevated Ang-2 concentrations are associated with all-cause mortality in male {hazard ratio [HR] 3.294 [95% confidence interval (CI) 1.768–6.138]; P = 0.0002} but not in female end-stage kidney disease patients on HD [HR 1.084 (95% CI 0.476–2.467); P = 0.847].

Conclusion

Ang-2 at baseline is independently associated with all-cause mortality in male ESRD patients on HD.
The N-terminally truncated helper NLR NRG1C antagonizes immunity mediated by its full-length neighbors NRG1A and NRG1B
Authors: Wu Zhongshou , Tian Lei , Liu Xueru , Huang Weijie , Zhang Yuelin , Li Xin
Published: November 2021

Both plants and animals utilize nucleotide-binding leucine-rich repeat immune receptors (NLRs) to perceive the presence of pathogen-derived molecules and induce immune responses. NLR genes are far more abundant and diverse in vascular plants than in animals. Truncated NLRs, which lack one or more of the canonical domains, are also commonly encoded in plant genomes. However, little is known about their functions, especially the N-terminally truncated ones. Here, we show that the Arabidopsis thaliana N-terminally truncated helper NLR (hNLR) gene N REQUIREMENT GENE1 (NRG1C) is highly induced upon pathogen infection and in autoimmune mutants. The immune response and cell death conferred by some Toll/interleukin-1 receptor-type NLRs (TNLs) were compromised in Arabidopsis NRG1C overexpression lines. Detailed genetic analysis revealed that NRG1C antagonizes the immunity mediated by its full-length neighbors NRG1A and NRG1B. Biochemical tests suggested that NRG1C might interfere with the EDS1–SAG101 complex, which functions in immunity signaling together with NRG1A/1B. Interestingly, Brassicaceae NRG1Cs are functionally exchangeable and that the Nicotiana benthamiana N-terminally truncated hNLR NRG2 also antagonizes NRG1 activity. Together, our study uncovers an unexpected negative role of N-terminally truncated hNLRs in immunity in different plant species.
Combination of cycle threshold time, absolute lymphocyte count and neutrophil:lymphocyte ratio is predictive of hypoxia in patients with SARS-CoV-2 infection
Authors: Abeysuriya Visula , Seneviratne Suranjith L , de Silva Arjuna P , Mowjood Riaz , Mowjood Shazli , de Silva Thushara , de Mel Primesh , de Mel Chandima , Chandrasena Lal , Wijesinha R S , Fernando Amitha , de Mel Sanjay
Published: November 2021

Background

There is currently no clinically validated biomarker to predict respiratory compromise in sudden acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Cycle threshold time (Ct), absolute lymphocyte count (AL) and neutrophil:lymphocyte ratio (NLR) have been previously evaluated for this purpose. We hypothesized that the combination of these parameters at presentation may be predictive of hypoxia (oxygen saturation <92%).

Methods

Data were collected on 118 patients with SARS-CoV-2 infection between May 2020 and April 2021. Demographics, clinical parameters and laboratory and radiological investigation results were recorded. Respiratory compromise (RC) was defined based on symptoms and signs, hypoxia and chest X-ray abnormalities.

Results

RC occurred in 61 (51.7%) of patients. The Ct, AL and NLR at median day 3 of illness were significantly different between patients with and without RC (Ct, RC vs not: 19.46±2.64 vs 22.62±3.37, p=0.0001; AL, RC vs not: 531.49±289.09 vs 764.69±481.79, p=0.0001; NLR, RC vs not: 3.42±0.75 vs 2.59±0.55, p=0.0001). Receiver operating characteristics analysis showed that a Ct <19.9, AL <630.8×103/μL and NLR >3.12 at median day 3 of symptoms was predictive of hypoxia on day 7 of illness (area under the curve 0.805, sensitivity 96.7%, specificity 69.1%). The predictive value for the parameters combined was significantly superior to their individual predictive power.

Conclusions

Ct, AL and NLR used in combination on day 3 of symptoms are predictive of hypoxia on day 7 of SARS-CoV-2 illness.
Deaths related to Chagas disease and HIV/AIDS coinfection in Brazil: a nationwide population-based analysis
Authors: Martins-Melo Francisco Rogerlândio , Castro Marcia C , Werneck Guilherme Loureiro , Heukelbach Jorg
Published: November 2021

Background

Trypanosoma cruzi/HIV coinfection has been described as a relevant clinical event and an emerging public health problem. Here, we describe the epidemiological patterns of deaths related to Chagas disease and HIV/AIDS coinfection in Brazil from 2000 to 2019.

Methods

We performed a nationwide population-based study using mortality data obtained from the Brazilian Mortality Information System. We included all deaths recorded in Brazil from 2000 to 2019 in which Chagas disease and HIV/AIDS were mentioned on the same death certificate, either as underlying or as associated causes of death.

Results

Chagas disease and HIV/AIDS were mentioned on 196/22 663 092 death certificates. HIV/AIDS was the underlying cause in 58.2% (114/196) of deaths and Chagas disease in 33.2% (65/196). The average annual mortality rate was 0.05 deaths/1 000 000 inhabitants (95% CI 0.03 to 0.09). The highest death rates were found among males, those aged 60–69 y, Afro-Brazilians, those with 1–3 y of schooling/study and residents in Chagas disease-endemic regions/states. Respiratory, infectious/parasitic and cardiovascular diseases/disorders were the associated causes of death most commonly mentioned.

Conclusions

Mortality due to Chagas disease and HIV/AIDS coinfection may be largely underestimated in Brazil. Our data further reinforce the importance of screening for T. cruzi infection in HIV-infected patients from Chagas disease-endemic areas. Appropriate clinical management should be ensured for Chagas disease and HIV coinfected patients.
Superior capsular reconstruction for management of massive irreparable rotator cuff tears: current concepts
Authors: McGovern Madeline M. , Bassett Ashley J.
Published: November 2021

Irreparable massive rotator cuff tears in active middle-aged patients result in significant shoulder dysfunction and challenge surgeons in their treatment selection. Superior capsular reconstruction (SCR) is a joint-preserving surgical option that restores glenohumeral stability and prevents superior humeral head migration, thereby improving shoulder function, lessening pain, and limiting development of rotator cuff arthropathy. Indications for SCR are irreparable supraspinatus and/or infraspinatus tears with persistent shoulder pain and/or dysfunction for which conservative treatment has failed in a patient who is not an ideal candidate for arthroplasty because of age or activity level. Patients must have relatively preserved glenohumeral cartilage, an intact or repairable subscapularis tendon, and a fully functioning deltoid muscle to be an appropriate candidate for SCR. The procedure was originally described by Dr. Teruhisa Mihata using fascia lata autograft; however, in North America, dermal allograft has become the most common graft choice. The graft is secured medially to the superior glenoid with two or three suture anchors and laterally to the tuberosity commonly using four anchors in a double-row configuration. Preliminary clinical studies demonstrate significant improvement in pain, function, muscle strength, active shoulder range of motion with resolution of pseudoparalysis, normalization of the acromiohumeral interval, and no rapid progression of osteoarthritis. Superior capsular reconstruction is a viable, joint-sparing surgical option for irreparable massive rotator cuff tears.
Primary tumor location in lung cancer: the evaluation and administration
Authors: Xie Xueqi , Li Xiaolin , Tang Wenjie , Xie Peng , Tan Xuefen
Published: November 2021

Lung cancer continues to be the leading cause of cancer-related death in the world, which is classically subgrouped into two major histological types: Non-small cell lung cancer (NSCLC) (85% of patients) and small-cell lung cancer (SCLC) (15%). Tumor location has been reported to be associated with the prognosis of various solid tumors. Several types of cancer often occur in a specific region and are more prone to spread to predilection locations, including colorectal cancer, prostate cancer, gastric cancer, ovarian cancer, cervical cancer, bladder cancer, lung tumor, and so on. Besides, tumor location is also considered as a risk factor for lung neoplasm with chronic obstructive pulmonary disease/emphysema. Additionally, the primary lung cancer location is associated with specific lymph node metastasis. And the recent analysis has shown that the primary location may affect metastasis pattern in metastatic NSCLC based on a large population. Numerous studies have enrolled the “location” factor in the risk model. Anatomy location and lobe-specific location are both important in prognosis. Therefore, it is important for us to clarify the characteristics about tumor location according to various definitions. However, the inconsistent definitions about tumor location among different articles are controversial. It is also a significant guidance in multimode therapy in the present time. In this review, we mainly aim to provide a new insight about tumor location, including anatomy, clinicopathology, and prognosis in patients with lung neoplasm.
Home Environment Influence on Adolescent Health Literacy
Authors: Melton Karen K. , Caldwell Elizabeth Perry
Published: November 2021

Adolescent health disparities are influenced by individuals' health literacy. To date, the only known household factors to influence adolescent health literacy (AHL) are social capital factors of parental health literacy, parent education, and household income. Therefore, the purpose of this study was to expand the understanding of household factors that influence AHL for future interventions. A sample of 105 adolescents and their parents completed an online survey. Home environment variables included family communication, family involvement, and books in the home. A quantitative analysis of correlations and regression was employed to explore the relationship between AHL and household factors. Findings from this study suggest that parental health literacy is the best-known household facilitator of AHL. Family communication and family involvement were not correlated with AHL. The number of books in the home was correlated with AHL. A good understanding of the factors influencing AHL is necessary for developing interventions. These findings continue to lend support that AHL is heavily associated with parental health literacy. Based on the salience of these findings in the research, future health literacy interventions should consider incorporating a parent/caregiver component. Yet, what remains unknown is the mechanism between AHL and parental health literacy.
Select immunizations for adult women
Authors: Brown Vivien
Published: November 2021

National standards for immunizations are set by the Advisory Committee on Immunization Practices in the United States and the National Advisory Committee on Immunizations in Canada. In Mexico, the National Immunization Technical Advisory Group sets standards for infants and adolescents but not for adults. In this Practice Pearl, guidelines for some adult immunizations in women and the recent and potential upcoming changes in vaccines are reviewed.
Anatomical Study of the Dorsal Nasal Artery to Prevent Visual Complications during Dorsal Nasal Augmentation
Authors: Tansatit Tanvaa , Jitaree Benrita , Uruwan Sukanya , Rungsawang Chalermquan
Published: November 2021

Dorsal nasal augmentation is a common injection associated with ocular complications. Digital compressions on both sides of the nose are recommended during injection. Considering the reported incidences of visual complications, this preventive technique may need an adjustment for more effectiveness to prevent blindness. Therefore, the dorsal nasal arteries (DNAs) were studied by conventional dissections in the subcutaneous and fibromuscular tissues of the nasal dorsum in 60 embalmed cadavers. The results showed that among the 60 faces, 32 faces had bilateral DNAs (53.3%), 23 had dorsal nasal plexus with minute arteries (38.3%), and five had a single dominant DNA (8.3%). The DNA originated from one of the four arterial sources, which influenced the location and course of the artery. These sources included the ophthalmic angular arteries in 21 faces (56.8%), terminal ophthalmic arteries in two faces (5.4%), lateral nasal arteries in 11 faces (29.7%) and facial angular arteries in three faces (8.1%). Consequently, the dominant dorsal nasal artery running close to the midline found in 8% of the cases could make side compressions during nasal dorsum augmentation less effective from preventing ocular complications. However, an adjustment of digital compressions which combines pinching and side compressions is suggested to improve the safety.
The Octopus Procedure Combined with Targeted Muscle Reinnervation for Elective Transhumeral Amputation
Authors: Agrawal Nikhil , Olafsson Sigurast , Pickrell Brent B. , Heng Marilyn , Valerio Ian L. , Eberlin Kyle R.
Published: November 2021

Optimizing prosthetic function and tolerance are key principles of performing an elective upper extremity amputation. It is common for upper extremity amputees to experience issues related to nonoptimal prosthetic control and pain. Targeted muscle reinnervation and regenerative peripheral nerve interfaces in elective transhumeral amputations have been introduced as techniques to address the paucity of signals that may exist for myoelectric control postamputation. These techniques require the denervation of muscle and rely on delayed muscle reinnervation to provide eventual signal amplification for prosthetic function. In addition, the fascicles cannot be separated enough to provide signals to each individual muscle. Use of native innervated forearm musculature can provide more immediate and specific signals for prosthetic use. These native muscles are often not available for use due to trauma, denervation, or dysvascularization. In elective amputations, they can be used as spare parts to provide more signals for the sensors on a myoelectric prosthetic. The concept has been used in partial hand amputations and allowed for individual digital control at the terminal prosthetic device. In this study, we describe a novel technique used for an elective transhumeral amputation utilizing native innervated, vascularized musculature to provide intuitive control of a myoelectric prosthetic.
Higher aldosterone is associated with increased renal impairment risk in patients with hypertension and abnormal glucose metabolism: a longitudinal study
Authors: Lin Mengyue , Heizhati Mulalibieke , Gan Lin , Yao Xiaoguang , Luo Qin , Zhang Delian , Abulikemu Suofeiya , Wang Menghui , Wang Guoliang , Jiang Wen , Hu Junli , Maimaiti Nuerguli , Wang Lei , Wu Ting , Sun Le , Yue Na , Ren Yingli , Li Nanfang
Published: November 2021

Objective:

The aim of this study was to evaluate the association between plasma aldosterone concentration (PAC) and renal impairment in patients with both hypertension and abnormal glucose metabolism (AGM).

Methods:

The longitudinal observational study included 2033 hypertensive individuals with AGM who did not have chronic kidney disease (CKD) at baseline. CKD was defined as estimated glomerular filtration rate (eGFR) less than 60 ml/min per 1.73 m2 and/or positive proteinuria. Directed acyclic graphs and LASSO regression analyses were applied to identify adjusted sets. Cox proportional hazard models and linear regression were used to evaluate the association of PAC with CKD and its components including decreased renal function (DRF) and proteinuria. Mediation analysis was used to examine the role of blood pressure (BP) in the association between the two.

Results:

During total follow-up of 5951 person-years with a median follow-up of 31 months, 291 participants developed CKD. The incidence of CKD was increased with the elevation in tertile PAC. Multivariable Cox model showed that PAC was positively associated with increased CKD risk (hazard ratio = 1.76 for natural log-transformed PAC, P < 0.001), and with increased risk of DRF and proteinuria. SBP mediated 7.5–17.9% of the association between PAC and renal impairment. Overall results remained consistent and significant in sensitivity analysis by excluding those with suspicious primary aldosteronism, too short follow-up time and mineralocorticoid receptor antagonists use.

Conclusion:

Higher PAC was associated with increased CKD risk in patients with hypertension and AGM, even in the absence of suspicious primary aldosteronism. The results indicate PAC may serve as a potential therapeutic target in this population.
Comprehensive biomarker profiling of hypertension in 36 985 Finnish individuals
Authors: Palmu Joonatan , Tikkanen Emmi , Havulinna Aki S. , Vartiainen Erkki , Lundqvist Annamari , Ruuskanen Matti O. , Perola Markus , Ala-Korpela Mika , Jousilahti Pekka , Würtz Peter , Salomaa Veikko , Lahti Leo , Niiranen Teemu
Published: November 2021

Objective:

Previous studies on the association between metabolic biomarkers and hypertension have been limited by small sample sizes, low number of studied biomarkers, and cross-sectional study design. In the largest study to date, we assess the cross-sectional and longitudinal associations between high-abundance serum biomarkers and blood pressure (BP).

Methods:

We studied cross-sectional (N = 36 985; age 50.5 ± 14.2; 53.1% women) and longitudinal (N = 4197; age 49.4 ± 11.8, 55.3% women) population samples of Finnish individuals. We included 53 serum biomarkers and other detailed lipoprotein subclass measures in our analyses. We studied the associations between serum biomarkers and BP using both conventional statistical methods and a machine learning algorithm (gradient boosting) while adjusting for clinical risk factors.

Results:

Fifty-one of 53 serum biomarkers were cross-sectionally related to BP (adjusted P < 0.05 for all). Conventional linear regression modeling demonstrated that LDL cholesterol, remnant cholesterol, apolipoprotein B, and acetate were positively, and HDL particle size was negatively, associated with SBP change over time (adjusted P < 0.05 for all). Adding serum biomarkers (cross-sectional root-mean-square error: 16.27 mmHg; longitudinal: 17.61 mmHg) in the model with clinical measures (cross-sectional: 16.70 mmHg; longitudinal 18.52 mmHg) improved the machine learning model fit. Glucose, albumin, triglycerides in LDL, glycerol, VLDL particle size, and acetoacetate had the highest importance scores in models related to current or future BP.

Conclusion:

Our results suggest that serum lipids, and particularly LDL-derived and VLDL-derived cholesterol measures, and glucose metabolism abnormalities are associated with hypertension onset. Use of serum metabolite determination could improve identification of individuals at high risk of developing hypertension.
Effect of Complementary and Integrative Treatments on Fatigue Symptoms in Hemodialysis Patients
Authors: Bayülgen Melek Yeşil , Gün Meral
Published: November 2021

Although hemodialysis treatment is one of the main treatment methods in chronic kidney failure, it also causes many health problems. A variety of complementary and integrative therapies are used to relieve or minimize hemodialysis symptoms. Fatigue (60%-97%) is also one of these problems. Therefore, we have conducted a systematic review to determine the effect of complementary and integrative therapies on fatigue in hemodialysis patients. Relevant studies published from 2005 to 2019, in PubMed, EBSCOhost, ScienceDirect, and Cochran Library databases have been reviewed. In the study, initially, 21 039 pieces of data were obtained, and then 14 randomized controlled studies meeting the inclusion criteria were reviewed. The methodological qualities of the articles included in the study were determined by using the quality evaluation checklist in Jadad randomized controlled studies. In the literature, acupressure, aromatherapy, reflexology, massage, and yoga practice were found to be applied to hemodialysis patients and be effective in reducing pain and anxiety as well as improving sleep quality. It is recommended that these methods be included in the routine clinical care of patients receiving hemodialysis.
Self-Caring Practices Among Nurses
Authors: Davis Kim C. , Duffy Joanne R. , Marchessault Patricia , Miles Debra
Published: November 2021

Faith community nurses receive additional education in holistic health and intentional spiritual care, including self-caring. This study used an online survey to explore self-caring practices of faith community nurses and clinical nurses. Despite sample limitations, faith community nurses reported significantly higher levels of self-caring practices than clinical nurses.
SATB2 Immunopositivity in Spindle Cell (Sarcomatoid) Squamous Cell Carcinoma: A Potential Pitfall in Diagnosis
Authors: Sasidharan Anju , Kakkar Aanchal , Thakar Alok , Deo Suryanarayan V.S.
Published: November 2021

Spindle cell squamous cell carcinomas (SpSCC) are aggressive neoplasms constituting 1% of oral cavity tumors. A proportion of SpSCC do not stain with epithelial markers, and frequently express mesenchymal markers, viz. Vimentin, smooth muscle actin, muscle specific actin, S100 and desmin, confounding the diagnosis. Immunoexpression of SATB2, a transcription factor indicating osteoblastic lineage, has not been evaluated in SpSCC previously. We therefore performed SATB2 immunohistochemistry in 15 cases of SpSCCs and scored them with respect to intensity and percentage of tumor cells stained. SATB2 immunopositivity was identified in 9/15 (60%) SpSCCs, with varying intensity and distribution. Eight cases (53.3%) showed nonfocal staining of moderate to strong intensity, and 1 case (6.7%) showed focal weak staining. Of these, 3 cases (3/9; 33.33%) did not stain with epithelial/squamous markers. Thus, a subset of SpSCC demonstrate SATB2 immunopositivity. In oral tumors with bone involvement, SATB2 positivity may lead away from the diagnosis of SpSCC. Knowledge of this aberrant immunostaining is, therefore, extremely relevant to guard against misdiagnosis as osteosarcoma, particularly on biopsies which lack adjacent dysplastic epithelium, in cases which are monophasic spindle cell, and in those that do not show immunopositivity for epithelial/ squamous markers. Our results emphasize that an appropriate panel and not a single immunomarker is required to distinguish SpSCC from mesenchymal tumors including osteosarcoma.
Transition Home Following New Fecal Ostomy Creation
Authors: Keng Christine J. , Lee Jennie , Valencia Marlie , McKechnie Tyler , Forbes Shawn , Eskicioglu Cagla
Published: November 2021

PURPOSE:

Perioperative ostomy education is essential for patients to develop skills and comfort with self-care at home with a new ostomy, but shortened hospital length of stay (LOS) reduces time for postoperative education for patients. This study explored the initial experiences and care needs of patients who have undergone creation of a new ostomy during their transition from hospital to home.

DESIGN:

Qualitative interpretive description using latent content analysis.

SUBJECTS AND SETTING:

Thirteen patients who had undergone an elective colorectal surgery involving creation of an ostomy were interviewed. Participants were 33 to 78 years of age, 54% were female, and 62% were undergoing ostomy creation for colorectal cancer operations. Eleven participants underwent temporary ileostomy creation, and 2 patients had permanent end colostomies created.

METHODS:

Interviews were conducted in person and audiotaped by study investigators within 4 to 6 weeks postoperatively. Audiotapes were transcribed verbatim by trained transcriptionists, and each transcript was reviewed in duplicate by study investigators. A latent content analysis method was used to determine the implied meaning in participants' experiences of having a new ostomy.

RESULTS:

The transition from hospital to home with a new ostomy was illustrated by 5 major themes: (1) Having an ostomy is a life-changing and bizarre experience; (2) Adjustment and adaptation occur through acceptance and self-reliance; (3) It's a hands-on thing: with the role of WOC nurse providing support in achieving independence; (4) Improved home care infrastructure is needed; and (5) Practical advice shared from experiential learning.

CONCLUSION:

Patients with new ostomies are motivated and able to cope with the ostomy and regain independence over a short period after surgery. Shorter LOS does not impede ostomy education so long as adequate home care and support group programs are available. Knowledge gained through this study will assist WOC nurses in managing patients' expectations and the planning and delivery of education to patients with newly created ostomies.
Evolving Evidence Supporting Use of Rectal Irrigation in the Management of Bowel Dysfunction
Authors: Rodriguez Gianna , Muter Paula , Inglese Gary , Goldstine Jimena V. , Neil Nancy
Published: November 2021

Disorders of bowel function are prevalent, particularly among patients with spinal cord injuries and other neurological disorders. An individual's bowel control significantly impacts quality of life, as predictable bowel function is necessary to actively and independently participate in everyday activities. For many patients with bowel dysfunction, initial lifestyle adjustments and other conservative therapeutic interventions (eg, digital stimulation, oral laxatives, suppositories) are insufficient to reestablish regular bowel function. In addition to these options, rectal irrigation (RI) is a safe and effective method of standard bowel care that has been used for several decades in adults and children suffering from bowel dysfunction associated with neurogenic or functional bowel etiologies. Rectal irrigation is an appropriate option when conservative bowel treatments are inadequate. Unlike surgical options, RI can be initiated or discontinued at any time. This report summarizes the clinical, humanistic, and economic evidence supporting the use of RI in clinical practice, noting features (eg, practical considerations, patient education) that can improve patients' success with RI treatment.
Executive Summary: Debridement
Authors: Rajhathy Erin M. , Chaplain Valérie , Hill Mary C. , Woo Kevin Y. , Parslow Nancy E.
Published: November 2021

Debridement is described in the literature as having a high level of clinical risk and may result in patient harm when performed by untrained nurses. As a result, specialized knowledge, skills, and competencies are required to initiate, direct, and perform safe and effective debridement. This executive summary provides an overview of Debridement: Canadian Best Practice Recommendations for Nurses from the Nurses Specialized in Wound, Ostomy and Continence Canada (NSWOCC). The primary objective of these recommendations is to positively influence patient outcomes and enhance safety. The 12 recommendations place the safety of the patient and nurse at the forefront and highlight the educational, competency, certification, preceptor/mentorship, and legal requirements for nurses to initiate, direct, and perform all methods of debridement. We designed these recommendations to be circulated and implemented widely by nurses of various professional levels across the continuum of care and advocate for organizations and government agencies to clearly define debridement in their policies and legislative regulations.