bluuebunny/crossref_metadata_2025 huggingface dataset:
Performance Evaluation for Repair of HSGc-C5 Carcinoma Cell Using Geant4-DNA
Authors: Sakata Dousatsu , Suzuki Masao , Hirayama Ryoichi , Abe Yasushi , Muramatsu Masayuki , Sato Shinji , Belov Oleg , Kyriakou Ioanna , Emfietzoglou Dimitris , Guatelli Susanna , Incerti Sebastien , Inaniwa Taku
Published: December 2021

Track-structure Monte Carlo simulations are useful tools to evaluate initial DNA damage induced by irradiation. In the previous study, we have developed a Gean4-DNA-based application to estimate the cell surviving fraction of V79 cells after irradiation, bridging the gap between the initial DNA damage and the DNA rejoining kinetics by means of the two-lesion kinetics (TLK) model. However, since the DNA repair performance depends on cell line, the same model parameters cannot be used for different cell lines. Thus, we extended the Geant4-DNA application with a TLK model for the evaluation of DNA damage repair performance in HSGc-C5 carcinoma cells which are typically used for evaluating proton/carbon radiation treatment effects. For this evaluation, we also performed experimental measurements for cell surviving fractions and DNA rejoining kinetics of the HSGc-C5 cells irradiated by 70 MeV protons at the cyclotron facility at the National Institutes for Quantum and Radiological Science and Technology (QST). Concerning fast- and slow-DNA rejoining, the TLK model parameters were adequately optimized with the simulated initial DNA damage. The optimized DNA rejoining speeds were reasonably agreed with the experimental DNA rejoining speeds. Using the optimized TLK model, the Geant4-DNA simulation is now able to predict cell survival and DNA-rejoining kinetics for HSGc-C5 cells.
Correction: Meerz et al. Comparative Therapeutic Exploitability of Acute Adaptation Mechanisms to Photon and Proton Irradiation in 3D Head and Neck Squamous Cell Carcinoma Cell Cultures. Cancers 2021, 13, 1190
Authors: Meerz Annina , Deville Sara Sofia , Müller Johannes , Cordes Nils
Published: December 2021

The authors wish to make the following corrections to this paper [...]
Histopathological and Molecular Profiling of Clear Cell Sarcoma and Correlation with Response to Crizotinib: An Exploratory Study Related to EORTC 90101 “CREATE” Trial
Authors: Lee Che-Jui , Modave Elodie , Boeckx Bram , Stacchiotti Silvia , Rutkowski Piotr , Blay Jean-Yves , Debiec-Rychter Maria , Sciot Raf , Lambrechts Diether , Wozniak Agnieszka , Schöffski Patrick
Published: December 2021

Clear cell sarcoma (CCSA) is characterized by a chromosomal translocation leading to EWSR1 rearrangement, resulting in aberrant transcription of multiple genes, including MET. The EORTC 90101 phase II trial evaluated the MET inhibitor crizotinib in CCSA but resulted in only sporadic responses. We performed an in-depth histopathological and molecular analysis of archival CCSA samples to identify alterations potentially relevant for the treatment outcome. Immunohistochemical characterization of MET signaling was performed using a tissue microarray constructed from 32 CCSA cases. The DNA from 24 available tumor specimens was analyzed by low-coverage whole-genome sequencing and whole-exome sequencing for the detection of recurrent copy number alterations (CNAs) and mutations. A pathway enrichment analysis was performed to identify the pathways relevant for CCSA tumorigenesis. Kaplan–Meier estimates and Fisher’s exact test were used to correlate the molecular findings with the clinical features related to crizotinib treatment, aiming to assess a potential association with the outcomes. The histopathological analysis showed the absence of a MET ligand and MET activation, with the presence of MET itself in most of cases. However, the expression/activation of MET downstream molecules was frequently observed, suggesting the role of other receptors in CCSA signal transduction. Using sequencing, we detected a number of CNAs at the chromosomal arm and region levels. The most common alteration was a gain of 8q24.21, observed in 83% of the cases. The loss of chromosomes 9q and 12q24 was associated with shorter survival. Based on exome sequencing, 40 cancer-associated genes were found to be mutated in more than one sample, with SRGAP3 and KMT2D as the most common alterations (each in four cases). The mutated genes encoded proteins were mainly involved in receptor tyrosine kinase signaling, polymerase-II transcription, DNA damage repair, SUMOylation and chromatin organization. Disruption in chromatin organization was correlated with longer progression-free survival in patients receiving crizotinib. Conclusions: The infrequent activation of MET may explain the lack of response to crizotinib observed in the majority of cases in the clinical trial. Our work describes the molecular heterogeneity in CCSA and provides further insight into the biology of this ultra-rare malignancy, which may potentially lead to better therapeutic approaches for CCSA.
Different Involvement of Band 3 in Red Cell Deformability and Osmotic Fragility—A Comparative GP.Mur Erythrocyte Study
Authors: Kuo Mei-Shin , Chuang Cheng-Hsi , Cheng Han-Chih , Lin Hui-Ru , Wang Jong-Shyan , Hsu Kate
Published: December 2021

GP.Mur is a clinically important red blood cell (RBC) phenotype in Southeast Asia. The molecular entity of GP.Mur is glycophorin B-A-B hybrid protein that promotes band 3 expression and band 3–AQP1 interaction, and alters the organization of band 3 complexes with Rh/RhAG complexes. GP.Mur+ RBCs are more resistant to osmotic stress. To explore whether GP.Mur+ RBCs could be structurally more resilient, we compared deformability and osmotic fragility of fresh RBCs from 145 adults without major illness (47% GP.Mur). We also evaluated potential impacts of cellular and lipid factors on RBC deformability and osmotic resistivity. Contrary to our anticipation, these two physical properties were independent from each other based on multivariate regression analyses. GP.Mur+ RBCs were less deformable than non-GP.Mur RBCs. We also unexpectedly found 25% microcytosis in GP.Mur+ female subjects (10/40). Both microcytosis and membrane cholesterol reduced deformability, but the latter was only observed in non-GP.Mur and not GP.Mur+ normocytes. The osmotic fragility of erythrocytes was not affected by microcytosis; instead, larger mean corpuscular volume (MCV) increased the chances of hypotonic burst. From comparison with GP.Mur+ RBCs, higher band 3 expression strengthened the structure of RBC membrane and submembranous cytoskeletal networks and thereby reduced cell deformability; stronger band 3–AQP1 interaction additionally supported osmotic resistance. Thus, red cell deformability and osmotic resistivity involve distinct structural–functional roles of band 3.
DNA Environment of Centromeres and Non-Homologous Chromosomes Interactions in Mouse
Authors: Spangenberg Victor , Losev Mikhail , Volkhin Ilya , Smirnova Svetlana , Nikitin Pavel , Kolomiets Oxana
Published: December 2021

Although the pericentromeric regions of chromosomes that are enriched in tandemly repeated satellite DNA represent a significant part of eukaryotic genomes, they remain understudied, which is mainly due to interdisciplinary knowledge gaps. Recent studies suggest their important role in genome regulation, karyotype stability, and evolution. Thus, the idea of satellite DNA as a junk part of the genome has been refuted. The integration of data regarding molecular composition, chromosome behaviour, and the details of the in situ organization of pericentromeric regions is of great interest. The objective of this work was a cytogenetic analysis of the interactions between pericentromeric regions from non-homologous chromosomes in mouse spermatocytes using immuno-FISH. We analysed two events: the associations between centromeric regions of the X chromosome and autosomes and the associations between the centromeric regions of the autosomal bivalents that form chromocenters. We concluded that the X chromosome forms temporary synaptic associations with different autosomes in early meiotic prophase I, which can normally be found until the pachytene–diplotene, without signs of pachytene arrest. These associations are formed between the satellite-DNA-rich centromeric regions of the X chromosome and different autosomes but do not involve the satellite-DNA-poor centromeric region of the Y chromosome. We suggest the hypothetical model of X chromosome competitive replacement from such associations during synaptic correction. We showed that the centromeric region of the X chromosome in association remains free of γH2Ax-dependent chromatin inactivation, while the Y chromosome is completely inactivated. This finding highlights the predominant role of associations between satellite DNA-rich regions of different chromosomes, including the X chromosome. We suppose that X-autosomal transient associations are a manifestation of an additional synaptic disorder checkpoint. These associations are normally corrected before the late diplotene stage. We revealed that the intense spreading conditions that were applied to the spermatocyte I nuclei did not lead to the destruction of stretched chromatin fibers of elongated chromocenters enriched in satellite DNA. The tight associations that we revealed between the pericentromeric regions of different autosomal bivalents and the X chromosome may represent the basis for a mechanism for maintaining the repeats stability in the autosomes and in the X chromosome. The consequences of our findings are discussed.
Sensorimotor Rehabilitation Promotes Vestibular Compensation in a Rodent Model of Acute Peripheral Vestibulopathy by Promoting Microgliogenesis in the Deafferented Vestibular Nuclei
Authors: Marouane Emna , El Mahmoudi Nada , Rastoldo Guillaume , Péricat David , Watabe Isabelle , Lapôtre Agnès , Tonetto Alain , Xavier Frédéric , Dumas Olivier , Chabbert Christian , Artzner Vincent , Tighilet Brahim
Published: December 2021

Acute peripheral vestibulopathy leads to a cascade of symptoms involving balance and gait disorders that are particularly disabling for vestibular patients. Vestibular rehabilitation protocols have proven to be effective in improving vestibular compensation in clinical practice. Yet, the underlying neurobiological correlates remain unknown. The aim of this study was to highlight the behavioural and cellular consequences of a vestibular rehabilitation protocol adapted to a rat model of unilateral vestibular neurectomy. We developed a progressive sensory-motor rehabilitation task, and the behavioural consequences were quantified using a weight-distribution device. This analysis method provides a precise and ecological analysis of posturolocomotor vestibular deficits. At the cellular level, we focused on the analysis of plasticity mechanisms expressed in the vestibular nuclei. The results obtained show that vestibular rehabilitation induces a faster recovery of posturolocomotor deficits during vestibular compensation associated with a decrease in neurogenesis and an increase in microgliogenesis in the deafferented medial vestibular nucleus. This study reveals for the first time a part of the underlying adaptative neuroplasticity mechanisms of vestibular rehabilitation. These original data incite further investigation of the impact of rehabilitation on animal models of vestibulopathy. This new line of research should improve the management of vestibular patients.
Nano-Micro-Structured 6%–8% YSZ Thermal Barrier Coatings: A Comprehensive Review of Comparative Performance Analysis
Authors: Kumar Amarnath , Moledina Jenna , Liu Yuan , Chen Kuiying , Patnaik Prakash C.
Published: December 2021

Beneficial properties achieved by nanostructuring effects in materials have generated tremendous interests in applications in surface engineering, especially in thermal barrier coatings (TBC). Limitations in conventional TBC processing for gas turbines and aero-propulsion systems have been exposed during past decades when rapid progress was made in nano-structuring coating research and developments. The present work is a comprehensive review of the current state of progress in nanostructured TBC (Ntbc) in reference to its microstructure, damage progression, failure mechanisms and a wide range of properties. The review aims to address the comparative performance analysis between the nanostructured and conventional (microstructured) 6–8 wt.% yttrium stabilized zirconia (YSZ) TBC systems. Oxidation resistance and sintering behavior in two TBCs are considered as the central focus of discussion. A few schematics are used to represent major microstructural features and failure progression. A performance analysis is performed for standard 2-layer, as well as functionally graded multilayer, TBC systems. A comparison of TBC characteristics processed by plasma spray and vapor deposition techniques is also made as reference. Compared to the sea of R&D efforts made for conventional TBC (Ctbc), limited experimental studies on Ntbc offers conflicting data, and prediction modeling and computational research are scarce.
How to Calculate Condensed Matter Electronic Structure Based on Multi-Electron Atom Semi-Classical Model
Authors: Chkhartishvili Levan
Published: December 2021

Atoms are proved to be semi-classical electronic systems in the sense of closeness of their exact quantum electron energy spectrum with that calculated within semi-classical approximation. Introduced semi-classical model of atom represents the wave functions of bounded in atom electrons in form of hydrogen-like atomic orbitals with explicitly defined effective charge numbers. The hydrogen-like electron orbitals of constituting condensed matter atoms are used to calculate the matrix elements of the secular equation determining the condensed matter electronic structure in the linear-combination-of-atomic-orbitals (LCAO) approach. Preliminary test calculations are conducted for boron B atom and diboron B2 molecule electron systems.
Genome-Wide Association Mapping of Seedling Vigor and Regrowth Vigor in Winter Wheat
Authors: Maulana Frank , Huang Wangqi , Anderson Joshua D. , Kumssa Tadele T. , Ma Xue-Feng
Published: December 2021

Seedling vigor and regrowth ability are important traits for the forage production of winter wheat. The objectives of this study were to map quantitative trait loci (QTL) associated with seedling vigor and regrowth vigor traits using a genome-wide association mapping study (GWAS). Seedling vigor and regrowth vigor were evaluated with shoot length, the number of shoots per plant and shoot dry weight per plant 45 days after planting and 15 days after cutting. A large phenotypic variation was observed for all the traits studied. In total, 12 significant QTL for seedling vigor and 16 for regrowth vigor traits were detected on various chromosomes. Four QTL on chromosomes 2B, 4B, 5A and 7A for seedling vigor co-localized with QTL for regrowth vigor due to significant correlations between corresponding traits of the initial growth and regrowth. A BLAST search using DNA sequences of the significant loci revealed candidate genes playing roles in vegetative and reproductive development in different crop species. The QTL and single-nucleotide polymorphism (SNP) markers identified in this study will be further validated and used for marker-assisted selection of the traits during forage wheat breeding.
Technology-Based Neurorehabilitation in Parkinson’s Disease—A Narrative Review
Authors: Möller Jens Carsten , Zutter Daniel , Riener Robert
Published: December 2021

This narrative review provides a brief overview of the current literature on technology-based interventions for the neurorehabilitation of persons with Parkinson’s disease (PD). The role of brain–computer interfaces, exergaming/virtual-reality-based exercises, robot-assisted therapies and wearables is discussed. It is expected that technology-based neurorehabilitation will gain importance in the management of PD patients, although it is often not clear yet whether this approach is superior to conventional therapies. High-intensity technology-based neurorehabilitation may hold promise with respect to neuroprotective or neurorestorative actions in PD. Overall, more research is required in order to obtain more data on the feasibility, efficacy and safety of technology-based neurorehabilitation in persons with PD.
Association between Fruit and Vegetable Intake and Physical Activity among Breast Cancer Survivors: A Longitudinal Study
Authors: Amireault Steve , Brunet Jennifer , Kurth Jordan D. , Fong Angela J. , Sabiston Catherine M.
Published: December 2021

This study examines the association between rates of change in daily fruit and vegetable intake and in weekly levels of moderate-to-vigorous intensity physical activity (MVPA) over a 15-month period in women following primary treatment completion for breast cancer. Breast cancer survivors (N = 199) self-reported fruit and vegetable intake and wore an accelerometer for 7 consecutive days to measure levels of MVPA on five occasions every 3 months. Multivariate latent growth modeling revealed that the rate of change in fruit and vegetable intake was not associated with the rate of change in levels of MVPA. Baseline (Mean = 3.46 months post-treatment) levels of MVPA were not associated with the rate of change of daily fruit and vegetable intake; likewise, baseline fruit and vegetable intake was not associated with the rate of change in levels of MVPA. Behavioral interventions promoting fruit and vegetable intake should not be assumed to yield concomitant effects in promoting MVPA or vice versa.
Yakemys multiporcata n. g. n. sp., a Large Macrobaenid Turtle from the Basal Cretaceous of Thailand, with a Review of the Turtle Fauna from the Phu Kradung Formation and Its Stratigraphical Implications
Authors: Tong Haiyan , Chanthasit Phornphen , Naksri Wilailuck , Ditbanjong Pitaksit , Suteethorn Suravech , Buffetaut Eric , Suteethorn Varavudh , Wongko Kamonlak , Deesri Uthumporn , Claude Julien
Published: December 2021

Yakemys multiporcata n. g. n. sp. is described on the basis of shell elements from the upper part of the Phu Kradung Formation (basal Cretaceous), Khorat Plateau, NE Thailand and assigned to Macrobaenidae. The new taxon is unusually large for an early macrobaenid (with an estimated carapace length about 70 cm) and is characterized by a large, rounded, low shell, the presence of a midline keel and numerous additional strong ridges on the carapace, the anterolateral margin upturned to form a gutter, posterolateral peripherals mesiolaterally expanded, narrow vertebrals, the vertebral 4 triangular and narrowed posteriorly, a greatly reduced plastron with a short bridge, an oval and elongate entoplastron with reduced ventral exposure, and strip-shaped epiplastra. The discovery of a macrobaenid turtle provides further support for an Early Cretaceous age for the upper part of the Phu Kradung Formation.
Spitz Nevus with Features of Clark Nevus, So-Called SPARK Nevus: Case Series Presentation with Emphasis on Cytological and Histological Features
Authors: Cimmino Antonietta , Cazzato Gerardo , Colagrande Anna , Maiorano Eugenio , Lospalluti Lucia , Ingravallo Giuseppe , Resta Leonardo
Published: December 2021

Background: SPARK nevus represents a little-known and characterized entity, with few case series available in the literature. Methods and results: we present a case series of 12 patients (6 F and 6 M) between January 2005 and December 2020 and conduct a review of the current literature. Ten articles were selected on the basis of the adopted inclusion criteria and the PRISMA guidelines. Conclusions: The definition of histopathological and dermoscopic criteria are important to allow for an agreement to be reached among dermopathologists, and for the development of a consensus on higher case studies. To our knowledge, there are not many case series in the literature, and ours is part of the attempt to increase the knowledge of an entity that remains little-known and characterized.
Design of a Gasification Reactor for Manufacturing and Operation in West Africa
Authors: Zoungrana Laetitia , Sidibé Sayon dit Sadio , Herman Benoît , Coulibaly Yézouma , Jeanmart Hervé
Published: December 2021

This paper introduces the design of a biomass gasification reactor with specific constraints for its manufacturing and operation in the West African conditions. The foreseen applications are the valorisation into heat and electricity of agricultural biomass residues. Rice husk is chosen as the reference fuel for the design. Local manufacturing is a key feature and the main focus of the design, as it allows us to reduce the capital costs and facilitate the maintenance. The design methodology is based on the conceptual approach proposed by Cross. This approach leads in several steps to a rational design choice based on the evaluation of different solutions. In this study, nine reactor types have been compared leading to a prototype that best suits the defined objectives such as a local manufacturing, a secure installation and a sufficient gas quality. From this conception approach, the Semi-Batch, Fixed-bed reactor with air Aspiration appears the most suitable. Its specific characteristics for the foreseen application are a power of 44 kW based on the syngas lower heating value, an average fuel consumption of 20.38 kg/h and an average air flow of 28.8 kg/h for optimal gasification. The gasifier resulting from the design methodology has been built. It is presented in the paper.
Characterization and Analysis of Household Solid Waste Composition to Identify the Optimal Waste Management Method: A Case Study in Hanoi City, Vietnam
Authors: Phuong Ngo , Yabar Helmut , Mizunoya Takeshi
Published: December 2021

A survey on household solid waste generation and physical composition was conducted in Hanoi City, the capital of Vietnam. The study sampled 110 households in specific areas with different population density and household scale. Household solid waste was classified into 13 main categories and 25 sub-categories. The results showed that average generation rate of waste in Hanoi City is 0.63 kg per person per day with a slightly higher rate in rural areas than urban ones. The largest proportion was food and garden waste at 78.9% followed by plastic and paper. Plastic waste was segregated into plastic and nylon, and nylon was double that of plastics in household solid waste. Compared to previous studies, this study found a higher portion of organic matter in the waste characterization that could be attributed to the changes in lifestyle patterns associated with COVID-19. This situation provides challenges and opportunities for introducing biomass technology to recover energy.
Sex on the Screen: A Content Analysis of Free Internet Pornography Depicting Mixed-Sex Threesomes from 2012–2020
Authors: Kulibert Danica , Moran James B. , Preman Sharayah , Vannier Sarah A. , Thompson Ashley E.
Published: December 2021

Viewing online pornography is common among US adults, with mixed-sex threesome (MST) videos being one of the top 10 most popular categories of pornography for both men and women. The current content analysis applied sexual script theory to understand the themes present in these mixed-sex threesome videos. Independent coders viewed a total of 50 videos (25 MMF and 25 FFM) at each timepoint (2012, 2015, 2020) and coded for different sexual behaviors and themes in each video. By examining both same-sex (female–female, male–male) and other-sex (female–male) behaviors, as well as themes of aggression and sexual initiation in different videos and across three timepoints, it was determined that other-sex behaviors are more common in MST videos than same-sex behaviors. Same-sex behaviors between two female actors were more common than same-sex behaviors between two male actors. Aggression was a common theme in videos, with male actors being more aggressive on average than female actors. Most of these trends did not change across 8 years, suggesting that the impacts of traditional sexual scripts are pervasive in pornography, even in current online content. Important implications for both researchers and clinical professionals are discussed.
Coupled Electrochemical-Thermal Simulations and Validation of Minichannel Cold-Plate Water-Cooled Prismatic 20 Ah LiFePO4 Battery
Authors: Akkaldevi Chaithanya , Chitta Sandeep Dattu , Jaidi Jeevan , Panchal Satyam , Fowler Michael , Fraser Roydon
Published: December 2021

This paper discusses the quantitative validation carried out on a prismatic 20 Ah LiFePO4 battery sandwiched between two minichannel cold-plates with distributed flow having a single U-turn. A two-way coupled electrochemical-thermal simulations are performed at different discharge rates (1–4 C) and coolant inlet temperatures (15–35 °C). The predicted battery voltage response at room temperature (22 °C) and the performance of the Battery Thermal Management System (BTMS) in terms of the battery surface temperatures (maximum temperature, Tmax and temperature difference, ΔT) have been analyzed. Additionally, temperature variation at ten different locations on the battery surface is studied during the discharge process. The predicted temperatures are compared with the measured data and found to be in close agreement. Differences between the predicted and measured temperatures are attributed to the assumption of uniform heat generation by the Li-ion model (P2D), the accuracy of electrochemical property input data, and the accuracy of the measuring tools used. Overall, it is suggested that the Li-ion model can be used to design the efficient BTMS at the cell level.
Systematic Design and Circuit Analysis of Lightning Impulse Voltage Generation on Low-Inductance Loads
Authors: Yutthagowith Peerawut , Kitcharoen Phattarin , Kunakorn Anantawat
Published: December 2021

The well-known circuit for the generation of lightning impulse voltage (LIV) on low-inductance loads was introduced by Glaninger in 1975, and the circuit component selection was proposed by Feser. However, the circuit and the approach for the component selection have some difficulties for which further adjustment is required for obtaining the waveform parameters according to the standard requirement. In this paper, an extended Glaninger’s circuit with an additional series resistor is proposed. Furthermore, a systematic design and circuit analysis of LIV generation for low-inductance loads are developed. With the help of a circuit simulator, the circuit analysis for the component selection is described. The validity of the proposed circuit was confirmed by some experimental results in comparison with the simulated ones. The proposed circuit and component selection provide not only the generation waveform according to the standard requirement but also other promising performances in terms of the wide inductance load range from 400 μH to 4 mH, a voltage efficiency of over 80%, an overshoot voltage of below 5%, an undershoot voltage of below 40%, and a maximum charging capacitance of 10 μF. From the simulated and experimental results, the proposed circuit and component selection approach is very useful for the LIV tests on low-inductance loads instead of using the conventional approach based on trial and error.
Assessing Tree Coverage and the Direct and Mediation Effect of Tree Diversity on Carbon Storage through Stand Structure in Homegardens of Southwestern Bangladesh
Authors: Rahman Md Mizanur , Kundu Gauranga Kumar , Kabir Md Enamul , Ahmed Heera , Xu Ming
Published: December 2021

Dealing with two major challenges, climate change mitigation and biodiversity loss, under the same management program, is more noteworthy than addressing these two separately. Homegardens, a sustainable agroforestry system and a home of diverse species, can be a possible choice to address these two issues. In this study, we assessed tree coverage, and the direct and indirect effects of tree diversity on carbon storage in different carbon pools through stand structure in homegardens of southwestern Bangladesh, using Sentinel 2 and field inventory data from 40 homesteads in eight villages. An unsupervised classification method was followed to assess homegardens’ tree coverage. We found a high tree coverage (24.34% of total area of Dighalia) in homesteads, with a high overall accuracy of 96.52%. The biomass and soil organic carbon (p < 0.05) varied significantly among the eight villages, while total carbon stock did not vary significantly (p > 0.05). Shannon diversity had both direct and indirect effects on biomass carbon, upper layer soil organic carbon and total carbon storage, while basal area mediated the indirect effect. Both basal area and tree height had positive effects on biomass carbon and total carbon storage, with basal area having the strongest effect. These findings suggest that we must maintain higher diversity and tree height in order to maximize and sustain carbon storage, where tree diversity increases stand basal area and improves total carbon storage (including soil organic) in homegardens. Therefore, privately managed homegardens could be a potential nature-based solution for biodiversity conservation and climate change mitigation in Bangladesh.
Monitoring of Beech Glued Laminated Timber and Delamination Resistance of Beech Finger-Joints in Varying Ambient Climates
Authors: Stolze Hannes , Schuh Mathias , Kegel Sebastian , Fürkötter-Ziegenbein Connor , Brischke Christian , Militz Holger
Published: December 2021

In this study, varying ambient climates were simulated in a test building by changing temperature and relative humidity. Beech glued laminated timber (glulam, Fagus sylvatica, L.) was freshly installed in the test building and monitoring of the change in wood moisture content of the glulam resulting from the variations in climate was carried out. Subsequently, finger-jointed beech specimens were exposed to the variations in relative humidity measured in the course of the monitoring experiment on a laboratory scale, and thus an alternating climate regime was derived from the conditions in the test building. Its influence on the delamination of the finger-joints was evaluated. In addition, it was examined whether beech finger-joints using commercial adhesive systems fulfil the normative requirements for delamination resistance according to EN 301 (2018) and whether different bonding-wood moisture levels have an effect on the delamination of the finger-joints. In the context of the monitoring experiment, there was a clear moisture gradient in the beech glulam between the inner and near-surface wood. The applied adhesive systems showed almost the same delamination resistance after variation of relative humidity. The normative requirements were met by all PRF-bonded and by most PUR-bonded beech finger-joints with higher bonding wood moisture content.
The Driving Forces of Anoplophora glabripennis Have Spatial Spillover Effects
Authors: Huang Jixia , Lu Xiao , Liu Hengzi , Zong Shixiang
Published: December 2021

Anoplophora glabripennis Motschulsky, 1854 (Asian longhorned beetle) does serious damage to forests. It has a long history and wide distribution area in China and is spreading there and elsewhere. Extreme climate events, such as cold surges and droughts, have had a promotive impact on Anoplophora glabripennis occurrence, but the spatial spillover effect of extreme climate events and other environmental factors on the occurrence of this pest has not yet been clarified. Two indices, namely, Standardized Precipitation Evapotranspiration Index (SPEI) and Low Temperature Index (LTI), were used to quantify the effects of drought and low-temperature freezing damage. Based on spatial panel data modeling, this study calculated the spatial spillover effect of environmental factors on the incidence of Anoplophora glabripennis in 666 counties in China’s central plains from 2002 to 2009. The factors examined included LTI, SPEI, average wind speed, hours of sunlight, Gross Domestic Product (GDP) of regional primary industry, population density, Normalized Difference Vegetation Index (NDVI), and pest control rate. Study results indicated that the impacts of environmental factors on the incidence rate of Anoplophora glabripennis are different. Low-temperature freezing damage, drought, wind speed, and pest control rate had a driving impact on pest incidence rates. Overall, the direct effect accounts for about 85% of the total effect, while the indirect effect accounts for about 15% of the total effect.
Effects of Lipase Addition, Hydrothermal Processing, Their Combination, and Co-Digestion with Crude Glycerol on Food Waste Anaerobic Digestion
Authors: Li Xiaojue , Shimizu Naoto
Published: December 2021

To enhance anaerobic fermentation during food waste (FW) digestion, pretreatments can be applied or the FW can be co-digested with other waste. In this study, lipase addition (LA), hydrothermal pretreatment (HTP), and a combination of both methods (HL) were applied to hydrolyze organic matter in FW. Furthermore, the effects of crude glycerol (CG), which provided 5%, 10%, and 15% of the volatile solids (VS) as co-substrate (denoted as CG5, CG10, and CG15, respectively), on the anaerobic digestion of FW were assessed. With an increasing proportion of CG in the co-digestion experiment, CG10 showed higher methane production, while CG15 negatively affected the anaerobic digestion (AD) performance owing to propionic acid accumulation acidifying the reactors and inhibiting methanogen growth. As the pretreatments partially decomposed hard-to-degrade substances in advance, pretreated FW showed a stronger methane production ability compared with raw FW, especially using the HL method, which was significantly better than co-digestion. HL pretreatment was shown to be a promising option for enhancing the methane potential value (1.773 NL CH4/g VS) according to the modified Gompertz model.
DNS Firewall Based on Machine Learning
Authors: Marques Claudio , Malta Silvestre , Magalhães João
Published: December 2021

Nowadays there are many DNS firewall solutions to prevent users accessing malicious domains. These can provide real-time protection and block illegitimate communications, contributing to the cybersecurity posture of the organizations. Most of these solutions are based on known malicious domain lists that are being constantly updated. However, in this way, it is only possible to block malicious communications for known malicious domains, leaving out many others that are malicious but have not yet been updated in the blocklists. This work provides a study to implement a DNS firewall solution based on ML and so improve the detection of malicious domain requests on the fly. For this purpose, a dataset with 34 features and 90 k records was created based on real DNS logs. The data were enriched using OSINT sources. Exploratory analysis and data preparation steps were carried out, and the final dataset submitted to different Supervised ML algorithms to accurately and quickly classify if a domain request is malicious or not. The results show that the ML algorithms were able to classify the benign and malicious domains with accuracy rates between 89% and 96%, and with a classification time between 0.01 and 3.37 s. The contributions of this study are twofold. In terms of research, a dataset was made public and the methodology can be used by other researchers. In terms of solution, the work provides the baseline to implement an in band DNS firewall.
Hybrid Uncertainty-Goal Programming Model with Scaled Index for Production Planning Assessment
Authors: Watada Junzo , Arbaiy Nureize Binti , Chen Qiuhong
Published: December 2021

Goal programming (GP) can be thought of as an extension or generalization of linear programming to handle multiple, normally conflicting objective measures. Each of these measures is given a goal or target value to be achieved. Unwanted deviations from this set of target values are then minimized in an achievement function. Production planning is an important process that aims to leverage the resources available in industry to achieve one or more business goals. However, the production planning that typically uses mathematical models has its own challenges where parameter models are sometimes difficult to find easily and accurately. Data collected with various data collection methods and human experts’ judgments are often prone to uncertainties that can affect the information presented by quantitative results. This study focuses on resolving data uncertainties as well as multi-objective optimization using fuzzy random methods and GP in production planning problems. GP was enhanced with fuzzy random features. Scalable approaches and maximum minimum operators were then used to solve multi-object optimization problems. Scaled indices were also introduced to resolve fuzzy symbols containing unspecified relationships. The application results indicate that the proposed approach can mitigate the characteristics of uncertainty in the analysis and achieve a satisfactory optimized solution.
Multilayered Complexity Analysis in Architectural Design: Two Measurement Methods Evaluating Self-Similarity and Complexity
Authors: Lorenz Wolfgang E. , Kulcke Matthias
Published: December 2021

This article contributes to clarifying the questions of whether and how fractal geometry, i.e., some of its main properties, are suitable to characterize architectural designs. This is done in reference to complexity-related aesthetic qualities in architecture, taking advantage of the measurability of one of them; the fractal dimension. Research in this area so far, has focused on 2-dimensional elevation plans. The authors present several methods to be used on a variety of source formats, among them a recent method to analyze pictures taken from buildings, i.e., 2.5-dimensional representations, to discuss the potential that lies within their combination. Color analysis methods will provide further information on the significance of a multilayered production and observation of results in this realm. In this publication results from the box-counting method are combined with a coordinate-based method for analyzing redundancy of proportions and their interrelations as well as the potential to include further layers of comparison are discussed. It presents a new area of box-counting implementation, a methodologically redesigned gradient analysis and its new algorithm as well as the combination of both. This research shows that in future systems it will be crucial to integrate several strategies to measure balanced aesthetic complexity in architecture.
Heather’s Homestead/Marotahei: The Invasion of the Waikato and Ways of Knowing Our Past in Aotearoa New Zealand
Authors: Campbell Hugh , Cuthers William Kainana
Published: December 2021

The British invasion of the Māori region of the Waikato in 1863 was one of the most pivotal moments in the colonisation of Aotearoa New Zealand. It has been the subject of multiple authoritative histories and sits at the centre of historical discussions of sovereignty, colonial politics and the dire consequences of colonisation. This article approaches this complex historical moment through the personal histories of a Māori/Pākehā homestead located at the political and geographic epicentre of the invasion. This mixed whanau/family provides the opportunity to explore a more kinship-based ontology of the invisible lines of influence that influenced particular actions before and during the invasion. It does so by mobilising two genealogical approaches, one by author Hugh Campbell which explores the British/Pākehā individuals involved in this family and uses formal documentation and wider historical writing to explain key dynamics—but also to expose a particular limitation of reliance on Western ontologies and formal documentation alone to explain histories of colonisation. In parallel to this approach, the other author—William Kainana Cuthers—uses both formal/Western and a Māori/Pasifika relational ontology of enquiry, and in doing so, allows both authors to open up a set of key insights into this pivotal moment in the history of Aotearoa New Zealand and into the micro-dynamics of colonisation.
HLA-DQA1*05 Associates with Extensive Ulcerative Colitis at Diagnosis: An Observational Study in Children
Authors: Nowak Jan Krzysztof , Glapa-Nowak Aleksandra , Banaszkiewicz Aleksandra , Iwańczak Barbara , Kwiecień Jarosław , Szaflarska-Popławska Anna , Grzybowska-Chlebowczyk Urszula , Osiecki Marcin , Kierkuś Jarosław , Hołubiec Magdalena , Chanaj-Kaczmarek Justyna , Radzikowski Andrzej , Walkowiak Jarosław
Published: December 2021

The human leukocyte antigen (HLA) allele group HLA-DQA1*05 predisposes to ulcerative colitis (UC) and is associated with the development of antibodies against infliximab in patients with inflammatory bowel disease (IBD). Therefore, we hypothesized that the presence of HLA-DQA1*05 correlates with characteristics of pediatric IBD. Within a multi-center cohort in Poland, the phenotype at diagnosis and worst flare was established and HLA-DQA1*05 status was assessed enabling genotype-phenotype analyses. HLA-DQA1*05 was present in 221 (55.1%) out of 401 children with IBD (UC n = 188, Crohn’s disease n = 213). In UC, the presence of HLA-DQA1*05 was moderately associated with a large extent of colonic inflammation at diagnosis (E4 55% more frequent in HLA-DQA1*05-positive patients, p = 0.012). PUCAI at diagnosis (p = 0.078) and the time from UC diagnosis to the first administration of biologic treatment (p = 0.054) did not differ depending on HLA-DQA1*05 status. The number of days of hospitalization for exacerbation was analyzed in 98 patients for whom sufficient follow-up was available and did not differ depending on HLA-DQA1*05 carriership (p = 0.066). HLA-DQA1*05 carriers with CD were less likely to present with both stenosing and penetrating disease (B2B3, p = 0.048) and to have active disease proximal to the ligament of Treitz (L4a) at the worst flare (p = 0.046). Future research focusing on explaining and preventing anti-TNF immunogenicity should take into account that ADA may develop not only as an isolated reaction to anti-TNF exposure but also as a consequence of intrinsic differences in the early course of UC.
Juvenile Amyotrophic Lateral Sclerosis: A Review
Authors: Lehky Tanya , Grunseich Christopher
Published: December 2021

Juvenile amyotrophic lateral sclerosis (JALS) is a rare group of motor neuron disorders with gene association in 40% of cases. JALS is defined as onset before age 25. We conducted a literature review of JALS and gene mutations associated with JALS. Results of the literature review show that the most common gene mutations associated with JALS are FUS, SETX, and ALS2. In familial cases, the gene mutations are mostly inherited in an autosomal recessive pattern and mutations in SETX are inherited in an autosomal dominant fashion. Disease prognosis varies from rapidly progressive to an indolent course. Distinct clinical features may emerge with specific gene mutations in addition to the clinical finding of combined upper and lower motor neuron degeneration. In conclusion, patients presenting with combined upper and lower motor neuron disorders before age 25 should be carefully examined for genetic mutations. Hereditary patterns and coexisting features may be useful in determining prognosis.
Examination of Genetic Variants Revealed from a Rat Model of Brain Ischemia in Patients with Ischemic Stroke: A Pilot Study
Authors: Khrunin Andrey V. , Khvorykh Gennady V. , Rozhkova Alexandra V. , Koltsova Evgeniya A. , Petrova Elizaveta A. , Kimelfeld Ekaterina I. , Limborska Svetlana A.
Published: December 2021

Although there has been great progress in understanding the genetic bases of ischemic stroke (IS), many of its aspects remain underexplored. These include the genetics of outcomes, as well as problems with the identification of real causative loci and their functional annotations. Therefore, analysis of the results obtained from animal models of brain ischemia could be helpful. We have developed a bioinformatic approach exploring single nucleotide polymorphisms (SNPs) in human orthologues of rat genes expressed differentially under conditions of induced brain ischemia. Using this approach, we identified and analyzed nine SNPs in 553 Russian individuals (331 patients with IS and 222 controls). We explored the association of SNPs with both IS outcomes and with the risk of IS. SNP rs66782529 (LGALS3) was associated with negative IS outcomes (p = 0.048). SNPs rs62278647 and rs2316710 (PTX3) were associated significantly with IS (p = 0.000029 and p = 0.0025, respectively). These correlations for rs62278647 and rs2316710 were found only in women, which suggests a sex-specific association of the PTX3 polymorphism. Thus, this research not only reveals some new genetic associations with IS and its outcomes but also shows how exploring variations in genes from a rat model of brain ischemia can be of use in searching for human genetic markers of this disorder.
Identification and Computational Analysis of Rare Variants of Known Hearing Loss Genes Present in Five Deaf Members of a Pakistani Kindred
Authors: Saleem Irum Badshah , Masoud Muhammad Shareef , Qasim Muhammad , Ali Muhammad , Ahmed Zubair M.
Published: December 2021

Hearing loss (HL) is the most common neurosensory defect in humans that affects the normal communication. Disease is clinically and genetically heterogeneous, rendering challenges for the molecular diagnosis of affected subjects. This study highlights the phenotypic and genetic complexity of inherited HL in a large consanguineous Pakistan kindred. Audiological evaluation of all affected individuals revealed varying degree of mild to profound sensorineural HL. Whole exome (WES) of four family members followed by Sanger sequencing revealed candidate disease-associated variants in five known deafness genes: GJB2 (c.231G>A; p.(Trp77 *)), SLC26A4 (c.1337A>G; p.(Gln446Arg)), CDH23 (c.2789C>T; p.(Pro930Leu)), KCNQ4 (c.1672G>A; p.(Val558Met)) and MPDZ (c.4124T>C; p.(Val1375Ala)). All identified variants replaced evolutionary conserved residues, were either absent or had low frequencies in the control databases. Our in silico and 3-Dimensional (3D) protein topology analyses support the damaging impact of identified variants on the encoded proteins. However, except for the previously established “pathogenic” and “likely pathogenic” categories for the c.231G>A (p.(Trp77 *)) allele of GJB2 and c.1377A>G (p.(Gln446Arg)) of SLC26A4, respectively, all the remaining identified variants were classified as “uncertain significance” based on the American College of Medical Genetics and Genomics/Association for Molecular Pathology (ACMG/AMP) variant pathogenicity guidelines. Our study highlights the complexity of genetic traits in consanguineous families, and the need of combining the functional studies even with the comprehensive profiling of multiple family members to improve the genetic diagnosis in complex inbred families.
Analysis of Serum Biochemical Indexes, Egg Quality, and Liver Transcriptome in Laying Hens Fed Diets Supplemented with Gynostemma pentaphyllum Powder
Authors: Li Tao , Zhang Shuya , Zhang Jiqiao , Song Yiping , Bao Xiuyu , Xu Fengwen , Zhang Jianqin
Published: December 2021

Gynostemma pentaphyllum (GP), known as “southern ginseng”, can reduce the blood pressure and blood lipid levels. In this study, 300 layer chicks of one day old were divided randomly into three groups (control group (base diet), high addition group (base diet with 1% GP), and low addition group (base diet with 0.5% GP)). After 29 weeks, the growth performance, egg quality, and serum index were determined. Additionally, liver mRNA was identified using RNA-seq to investigate the molecular mechanisms. The results indicated that the serum total cholesterol and triglycerides decreased significantly in the GP addition group. The addition of GP increased the egg weight, Haugh unit and redness (a*) of the egg yolk color, and reduced the yolk cholesterol concentration. Moreover, 95 differentially expressed genes (DEGs) were screened between the control and GP addition group. GO and the KEGG analysis showed that the PPAR pathway was significantly enriched. Five fatty acid metabolism-related genes (FABP3, CYP7A1, ANKRD22, SCD1, and PCK1) were validated by qRT-PCR analysis, which confirmed the tendency of the expression. These DEGs in the PPAR pathway may be the key factors of GP affecting fatty acid metabolism. These results may provide a theoretical basis for further research and new insights into GP as a feed additive.