bluuebunny/crossref_metadata_2025 huggingface dataset:
Highly Sensitive Temperature Sensors Resulting from the Luminescent Behavior of Sm3+-Doped Ba2MgMoO6 High-Symmetry Double-Perovskite Molybdate Phosphors
Authors: Miniajluk-Gaweł Natalia , Bondzior Bartosz , Ptak Maciej , Dereń Przemysław Jacek
Published: April 2024

We present double-perovskite molybdate with the formula of Ba2MgMoO6 doped with Sm3+ ions as a potential red phosphor to improve the color characteristics of white-light-emitting dioded (wLEDs). The new orange–red phosphor was synthesized using the co-precipitation (CP) method, and then its structural and spectroscopic properties were determined. Red emission at 642.6 nm dominates, which results from the electric dipole (ED) transition of the 4G5/2 → 6H9/2 type, and the materials are characterized by short luminescence decay times. BMM:Sm3+ is, to our best knowledge, the clearest example of dominant red emission of Sm3+ resulting from the location of the dopant in octahedral sites of high-symmetry cubic structure. In the sample containing 0.1% Sm3+, Sm3+ ions are located in both Mg2+ and Ba2+ sites, while at higher concentrations the Ba2+ site is less preferable for doping, as a result of which the emission becomes more uniform and single-site. The relative sensitivity calculated from FIR has a maximum of 2.7% K−1 at −30 °C and another local maximum of 1.6% K−1 at 75 °C. Such value is, to the best of our knowledge, one of the highest achieved for luminescent thermometry performed using only Sm3+ ions. To sum up, the obtained materials are good candidates as red phosphor to improve the color characteristics of wLEDs, obtaining a color-rendering index (CRI) of 91 and coordinated color temperature (CCT) of 2943 K, constituting a warm white emission. In addition to this, a promising precedent for temperature sensing using high-symmetry perovskite materials is the high sensitivity achieved, which results from the high symmetry of the BMM host.
Hyers–Ulam Stability of 2D-Convex Mappings and Some Related New Hermite–Hadamard, Pachpatte, and Fejér Type Integral Inequalities Using Novel Fractional Integral Operators via Totally Interval-Order Relations with Open Problem
Authors: Afzal Waqar , Breaz Daniel , Abbas Mujahid , Cotîrlă Luminiţa-Ioana , Khan Zareen A. , Rapeanu Eleonora
Published: April 2024

The aim of this paper is to introduce a new type of two-dimensional convexity by using total-order relations. In the first part of this paper, we examine the Hyers–Ulam stability of two-dimensional convex mappings by using the sandwich theorem. Our next step involves the development of Hermite–Hadamard inequality, including its weighted and product forms, by using a novel type of fractional operator having non-singular kernels. Moreover, we develop several nontrivial examples and remarks to demonstrate the validity of our main results. Finally, we examine approximate convex mappings and have left an open problem regarding the best optimal constants for two-dimensional approximate convexity.
Modeling the Propagation of Infectious Diseases across the Air Transport Network: A Bayesian Approach
Authors: Quirós Corte Pablo , Cano Javier , Sánchez Ayra Eduardo , Joshi Chaitanya , Gómez Comendador Víctor Fernando
Published: April 2024

The COVID-19 pandemic, caused by the SARS-CoV-2 virus, continues to impact the world even three years after its outbreak. International border closures and health alerts severely affected the air transport industry, resulting in substantial financial losses. This study analyzes the global data on infected individuals alongside aircraft types, flight durations, and passenger flows. Using a Bayesian framework, we forecast the risk of infection during commercial flights and its potential spread across an air transport network. Our model allows us to explore the effect of mitigation measures, such as closing individual routes or airports, reducing aircraft occupancy, or restricting access for infected passengers, on disease propagation, while allowing the air industry to operate at near-normal levels. Our novel approach combines dynamic network modeling with discrete event simulation. A real-case study at major European hubs illustrates our methodology.
Blockchain-Enabled Utility Optimization for Supply Chain Finance: An Evolutionary Game and Smart Contract Based Approach
Authors: Wang Shenghua , Zhou Mengjie , Xiang Sunan
Published: April 2024

In recent years, blockchain technology has attracted substantial interest for its capability to transform supply chain management and finance. This paper employs evolutionary game theory to investigate the application of blockchain in mitigating financial risks within supply chains, taking into account the technology’s maturity and the risk preferences of financial institutions. By modeling interactions among financial institutions, small and medium enterprises (SMEs), and core enterprises within the accounts receivable financing framework, this study evaluates blockchain’s impact on their decision-making and its efficacy in risk reduction. Our findings suggest the transformative potential of blockchain in mitigating financial risks, solving information asymmetry, and enhancing collaboration between financial entities and SMEs. Additionally, we integrate smart contracts into supply chain finance, proposing pragmatic procedures for their deployment in real-world contexts. Via a detailed examination of blockchain’s maturity and financial institutions’ risk preferences, this research demonstrates the primary determinants of strategic decisions in supply chain finance and underscores how blockchain technology fosters system stability using risk mitigation. Our innovative contribution lies in the design of smart contracts for the ARF process, rooted in blockchain’s core attributes of security, transparency, and immutability, thereby ensuring efficient operation and cost reduction in supply chain finance.
Numerical Simulation of Temperature Evolution, Solid Phase Transformation, and Residual Stress Distribution during Multi-Pass Welding Process of EH36 Marine Steel
Authors: Wen Pengyu , Wang Jiaji , Jiao Zhenbo , Fu Kuijun , Li Lili , Guo Jing
Published: April 2024

An investigation into the evolution of temperature and stress fields, as well as the phase transformation in marine steel EH36 during multi-pass welding, and their subsequent effects on Charpy impact toughness, remains in great lack. In this study, submerged arc welding (SAW) was employed to carry out multi-pass welding on EH36 steel plates, followed by the low-temperature toughness test of weldments. Comsol software version 6.2 and finite element analysis are utilized to simulate the evolution of the microstructure, temperature, and residual stress fields throughout the multi-pass welding process. As welding progressed, the heat absorption along the vertical direction was enhanced; in contrast, a decrease is observed in the horizontal direction away from the heat source. This complicated temperature history favors the bainite transformation in the vicinity to the heat source, whereas areas more remote from the weld zone exhibit a higher prevalence of acicular ferrite due to the reduced cooling rate. The concentration of residual stress is predicted to occur at the boundary of the melt pool and at the interface between the weld and the heat-affected zone, with the greatest deformation observed near the fusion line at the top surface of the model. Furthermore, multi-pass welding may alleviate the residual stress, especially when coupled with the formation of acicular ferrite upon cooling, leading to improved low-temperature impact toughness in regions remote from the heat source. These findings offer valuable insights for the design and optimization of multi-pass welding in future applications.
Geodynamic Settings of Late Paleozoic–Early Mesozoic Granitoid Magmatism at the Arctic Continental Margins: Insights from New Geochronological and Geochemical Data from the Taimyr Peninsula
Authors: Kurapov Mikhail Yu. , Konopelko Dmitry L. , Biske Yuriy S. , Proskurnin Vasiliy F. , Petrov Sergei V. , Proskurnina Maria A. , Berzon Yevgeny Yi. , Ershova Victoria B. , Berzin Stepan V. , Stepanov Sergey Yu.
Published: April 2024

Despite significant progress in Arctic geological studies, a number of principal questions concerning the Paleozoic collisional events remain unanswered. Therefore, the Taimyr Peninsula, representing the only outcropped high Arctic region where magmatic complexes, formed by Hercynian collision between the Siberian Craton and the Kara Block, are well exposed, is crucially important. In this paper we report new geochemical and geochronological data for intrusions in the poorly studied northeastern part of the Taimyr Peninsula. The obtained results in combination with published data show that supra-subduction magmatism at the southern active margin of the Kara Block continued from ca. 345 to 285 Ma (Early Carboniferous to Early Permian), and was followed by a post-collisional magmatic pulse that affected the whole Taimyr across terrane boundaries at ca. 280 Ma in the Early Permian. After cessation of the post-collisional magmatism at ca. 265 Ma, the Taimyr experienced extension, and voluminous magmatic series associated with a Siberian mantle plume were formed between 251 and 228 Ma during the Triassic. The studied post-collisional and plume-related intrusions of the Northeastern Taimyr are generally classified as evolved high-K I-type granites with adakitic affinity. The latter is a regional feature because the majority of the analyzed plume-related granitoids are geochemically similar to high potassium continental adakites. It is suggested that the adakitic geochemical characteristics of the plume-related granitoids resulted from melting of hydrated mafic lower crustal protoliths and were controlled by the source lithology. Comparison of the new results with data available for adjacent areas allows for correlation of terranes on a regional scale and sheds light on the evolution of the Arctic continental margins in general. In the Early–Middle Paleozoic, the Kara Block was part of a continental terrane that formed at the northern edge of Baltica as a result of Neoproterozoic Timanian orogeny. In the Early Carboniferous, the southern margin of Kara turned into an active margin, while its inferred continuation in the eastern Uralian margin of Baltica remained a passive margin until the Early Permian. This discrepancy can be explained by dextral displacement of Kara relative to Baltica that took place in the Early Carboniferous and was later accommodated by the formation of the Taimyr collisional belt in the course of the Early Permian collision between Kara and Siberia. After collision, the Taimyr was incorporated into the northern Eurasian margin as an uplifted block that experienced surface erosion and supplied clastic material in surrounding basins.
Research on Green Adsorbents
Authors: Wiśniewska Małgorzata , Nowicki Piotr
Published: April 2024

Adsorption processes play a crucial role in air purification, wastewater treatment, soil remediation technologies, noble metals recovery, and long-term energy storage systems [...]
Exploring the Pharmacological Potential of Lithospermum officinale L.: A Review of Phytochemicals and Ethnomedicinal Uses
Authors: Barkizatova Gulzhanat , Turgumbayeva Aknur , Zhakipbekov Kairat , Bekesheva Kuralay , Arystanov Zhalgaskali , Arystanova Tanagul , Kayupova Farida , Zhumalina Klara , Toxanbayeva Zhanat , Ibragimova Aigul , Blinova Olga , Utegenova Gulnara , Iztileu Nurzhan , Shynykul Zhanserik
Published: April 2024

Exploring phytochemicals from ethnomedicinal plants for pharmacological applications is a promising research area. By studying ethnomedicine, researchers can identify plants used for centuries to treat ailments and investigate their phytochemicals. Consequently, phytochemicals can be isolated, characterized, and tested for pharmacological activities, leading to new drug development. This research also helps preserve traditional knowledge and biodiversity. Lithospermum officinale L., found in Eurasia, Argentina (South), Colombia, and the United States, is valued for its medicinal properties, including anti-inflammatory, antioxidant, and antimicrobial effects. The current review emphasizes L. officinale L. as a significant reservoir of bioactive phytochemicals, with alkaloids, quinones, glucosides, phenolics, flavonoids, and lipids identified as the principal metabolites. It also unveils the unexplored potential of this plant for future research endeavors. Continued research on L. officinale L. can unlock its full potential, providing insights into its medicinal uses and contributing to biodiversity preservation.
Dissipation and Safety Analysis of Dimethomorph Application in Lychee by High-Performance Liquid Chromatography–Tandem Mass Spectrometry with QuEChERS
Authors: Wang Siwei , Wang Xiaonan , Liu Yanping , He Qiang , Tian Hai
Published: April 2024

This study presents a method for analyzing dimethomorph residues in lychee using QuEChERS extraction and HPLC-MS/MS. The validation parameters for this method, which include accuracy, precision, linearity, and recovery, indicate that it meets standard validation requirements. Following first-order kinetics, the dissipation dynamic of dimethomorph in lychee was determined to range from 6.4 to 9.2 days. Analysis of terminal residues revealed that residues in whole lychee were substantially greater than those in the pulp, indicating that dimethomorph residues are predominantly concentrated in the peel. When applied twice and thrice at two dosage levels with pre-harvest intervals (PHIs) of 5, 7, and 10 days, the terminal residues in whole lychee ranged from 0.092 to 1.99 mg/kg. The terminal residues of the pulp ranged from 0.01 to 0.18 mg/kg, with the residue ratio of whole lychee to pulp consistently exceeding one. The risk quotient (RQ) for dimethomorph, even at the recommended dosage, was less than one, indicating that the potential for damage was negligible. This study contributes to the establishment of maximum residue limits (MRLs) in China by providing essential information on the safe application of dimethomorph in lychee orchards.
Chemical Composition of Volatile and Extractive Components of Canary (Tenerife) Propolis
Authors: Isidorov Valery A. , Dallagnol Andrea M. , Zalewski Adam
Published: April 2024

The vegetation of the Canary Islands is characterized by a large number of endemic species confined to different altitudinal levels. It can be assumed that these circumstances determine the characteristic features of the chemical composition of local beekeeping products, including propolis. We report, for the first time, the chemical composition of propolis from Tenerife (Canary Islands). The volatile emissions of three propolis samples collected from different apiaries are represented by 162 C1–C20 compounds, of which 144 were identified using the HS-SPME/GC-MS technique. The main group of volatiles, consisting of 72 compounds, is formed by terpenoids, which account for 42–68% of the total ion current (TIC) of the chromatograms. The next most numerous groups are formed by C6–C17 alkanes and alkenes (6–32% TIC) and aliphatic C3–C11 carbonyl compounds (7–20% TIC). The volatile emissions also contain C1–C6 aliphatic acids and C2–C8 alcohols, as well as their esters. Peaks of 138 organic C3–C34 compounds were recorded in the chromatograms of the ether extracts of the studied propolis. Terpene compounds form the most numerous group, but their number and content in different samples is within very wide limits (9–63% TIC), which is probably due to the origin of the samples from apiaries located at different altitudes. A peculiarity of the chemical composition of the extractive substances is the almost complete absence of phenylcarboxylic acids and flavonoids, characteristic of Apis mellifera propolis from different regions of Eurasia and North America. Aromatic compounds of propolis from Tenerife are represented by a group of nine isomeric furofuranoid lignans, as well as alkyl- and alkenyl-substituted derivatives of salicylic acid and resorcinol.
Revealing the Critical Role of Global Electron Density Transfer in the Reaction Rate of Polar Organic Reactions within Molecular Electron Density Theory
Authors: Domingo Luis R. , Ríos-Gutiérrez Mar
Published: April 2024

The critical role of global electron density transfer (GEDT) in increasing the reaction rate of polar organic reactions has been studied within the framework of Molecular Electron Density Theory (MEDT). To this end, the series of the polar Diels–Alder (P-DA) reactions of cyclopentadiene with cyanoethylene derivatives, for which experimental kinetic data are available, have been chosen. A complete linear correlation between the computed activation Gibbs free energies and the GEDT taking place at the polar transition state structures (TSs) is found; the higher the GEDT at the TS, the lower the activation Gibbs free energy. An interacting quantum atoms energy partitioning analysis allows for establishing a complete linear correlation between the electronic stabilization of the electrophilic ethylene frameworks and the GEDT taking place at the polar TSs. This finding supports Parr’s proposal for the definition of the electrophilicity ω index. The present MEDT study establishes the critical role of the GEDT in the acceleration of polar reactions, since the electronic stabilization of the electrophilic framework with the electron density gain is greater than the destabilization of the nucleophilic one, making a net favorable electronic contribution to the decrease in the activation energy.
A Comprehensive Review of In Situ Measurement Techniques for Evaluating the Electro-Chemo-Mechanical Behaviors of Battery Electrodes
Authors: Jiang Hainan , Chen Jie , Li Xiaolin , Jin Zhiyao , Chen Tianjun , Liu Jiahui , Li Dawei
Published: April 2024

The global production landscape exhibits a substantial need for efficient and clean energy. Enhancing and advancing energy storage systems are a crucial avenue to optimize energy utilization and mitigate costs. Lithium batteries are the most effective and impressive energy utilization system at present, with good safety, high energy density, excellent cycle performance, and other advantages, occupying most of the market. However, due to the defects in the electrode material of the battery itself, the electrode will undergo the process of expansion, stress evolution, and electrode damage during electro-chemical cycling, which will degrade battery performance. Therefore, the detection of property changes in the electrode during electro-chemical cycling, such as the evolution of stress and the modulus change, are useful for preventing the degradation of lithium-ion batteries. This review presents a current overview of measurement systems applied to the performance detection of batteries’ electrodes, including the multi-beam optical stress sensor (MOSS) measurement system, the digital image correlation (DIC) measurement system, and the bending curvature measurement system (BCMS), which aims to highlight the measurement principles and advantages of the different systems, summarizes a part of the research methods by using each system, and discusses an effective way to improve the battery performance.
Detection and Identification of Pesticides in Fruits Coupling to an Au–Au Nanorod Array SERS Substrate and RF-1D-CNN Model Analysis
Authors: Sha Pengxing , Zhu Chushu , Wang Tianran , Dong Peitao , Wu Xuezhong
Published: April 2024

In this research, a method was developed for fabricating Au–Au nanorod array substrates through the deposition of large-area Au nanostructures on an Au nanorod array using a galvanic cell reaction. The incorporation of a granular structure enhanced both the number and intensity of surface-enhanced Raman scattering (SERS) hot spots on the substrate, thereby elevating the SERS performance beyond that of substrates composed solely of an Au nanorod. Calculations using the finite difference time domain method confirmed the generation of a strong electromagnetic field around the nanoparticles. Motivated by the electromotive force, Au ions in the chloroauric acid solution were reduced to form nanostructures on the nanorod array. The size and distribution density of these granular nanostructures could be modulated by varying the reaction time and the concentration of chloroauric acid. The resulting Au–Au nanorod array substrate exhibited an active, uniform, and reproducible SERS effect. With 1,2-bis(4-pyridyl)ethylene as the probe molecule, the detection sensitivity of the Au–Au nanorod array substrate was enhanced to 10−11 M, improving by five orders of magnitude over the substrate consisting only of an Au nanorod array. For a practical application, this substrate was utilized for the detection of pesticides, including thiram, thiabendazole, carbendazim, and phosmet, within the concentration range of 10−4 to 5 × 10−7 M. An analytical model combining a random forest and a one-dimensional convolutional neural network, referring to the important variable-one-dimensional convolutional neural network model, was developed for the precise identification of thiram. This approach demonstrated significant potential for biochemical sensing and rapid on-site identification.
Analysis of the Association between Protein Intake and Disability-Adjusted Life Year Rates for Alzheimer’s Disease in Japanese Aged over 60
Authors: Fujiwara Kazuki , Tanaka Takayuki , Kobayashi Hisamine , Nagao Kenji , Ishikawa-Takata Kazuko
Published: April 2024

With advancements in medical technology, the structure of disease is shifting from acute illnesses to chronic conditions, such as Alzheimer’s disease (AD). Consequently, there is an escalating need for evaluations that discourse on the potential effects on healthy life years, as well as disease onset. We aimed to evaluate the associations with AD disability-adjusted life year (AD-DALY) rates and protein intake by sex and age group. For the analysis, we used representative values for males and females in their 60s and aged over 70, extracted from the public dataset of the Global Burden of Disease Study and the National Health and Nutrition Survey in Japan, covering the years 1990 to 2019. In order to evaluate the association between AD-DALY rates and protein intake, we analyzed correlations and stratified multiple regression models. Additionally, we simulated alterations in AD-DALY rates associated with changes in protein intake by utilizing stratified multiple regression models. AD-DALY rates and protein intake indicated significant negative correlations across all sex and age groups. In stratified multiple regression models, significant associations were found between higher protein intake and lower AD-DALY rates in females. In the simulation, when protein intake was increased to 1.5 g/kg/day, AD-DALY rates decreased by 5–9 percent compared with 2019. However, the association between intake of animal and plant protein and AD-DALY rates were found to vary based on sex and age group. The present study suggests the possibility to improve AD-DALY rates by increasing population average protein intake levels in a recommended range.
A Prospective, Observational Study of the Effect of a High-Calorie, High-Protein Oral Nutritional Supplement with HMB in an Old and Malnourished or at-Risk-of-Malnutrition Population with Hip Fractures: A FracNut Study
Authors: Pareja Sierra Teresa , Hünicken Torrez Flavia , Pablos Hernández María , López Velasco Rosario , Ortés Gómez Raquel , Cervera Díaz María , Hormigo Sánchez Ana , Perdomo Ramírez Beatriz , Mora Fernández Jesús , Jiménez Mola Sonia , Rodriguez Piñera María , Condorhuaman Alvarado Patricia , Sanchez Juan Carlos , Ramos Clemente Juan , Veses Martín Silvia , Rodríguez Manzano Ingrid , González-Colaço Harmand Magali , Camprubí Robles María , Martín Aguilar Andrea , Saez Lopez Pilar
Published: April 2024

Background: Hip fractures are prevalent among older people, often leading to reduced mobility, muscle loss, and bone density decline. Malnutrition exacerbates the prognosis post surgery. This study aimed to evaluate the impact of a 12-week regimen of a high-calorie, high-protein oral supplement with β-hydroxy-β-methylbutyrate (HC-HP-HMB-ONS) on nutritional status, daily activities, and compliance in malnourished or at-risk older patients with hip fractures receiving standard care. Subjects and Methods: A total of 270 subjects ≥75 years of age, residing at home or in nursing homes, malnourished or at risk of malnutrition, and post hip fracture surgery, received HC-HP-HMB-ONS for 12 weeks. Various scales and questionnaires assessed outcomes. Results: During the 12 weeks of follow-up, 82.8% consumed ≥75% of HC-HP-HMB-ONS. By week 12, 62.4% gained or maintained weight (+0.3 kg), 29.2% achieved normal nutritional status (mean MNA score +2.8), and 46.8% improved nutritional status. Biochemical parameters improved significantly. Subjects reported good tolerability (mean score 8.5/10), with 87.1% of healthcare providers concurring. Conclusions: The administration of HC-HP-HMB-ONS markedly enhanced nutritional status and biochemical parameters in older hip-fracture patients, with high compliance and tolerability. Both patients and healthcare professionals expressed satisfaction with HC-HP-HMB-ONS.
The Therapeutic Potential of Four Main Compounds of Zanthoxylum nitidum (Roxb.) DC: A Comprehensive Study on Biological Processes, Anti-Inflammatory Effects, and Myocardial Toxicity
Authors: Li Xiaohan , Wang Qi , Liu Ling , Shi Yang , Hong Yang , Xu Wanqing , Xu Henghui , Feng Jing , Xie Minzhen , Li Yang , Yang Baofeng , Zhang Yong
Published: April 2024

Zanthoxylum nitidum (Roxb.) DC. (Z. nitidum) is a traditional Chinese medicinal plant that is indigenous to the southern regions of China. Previous research has provided evidence of the significant anti-inflammatory, antibacterial, and anticancer properties exhibited by Z. nitidum. The potential therapeutic effects and cardiac toxicity of Z. nitidum remain uncertain. The aim of this research was to investigate the potential therapeutic properties of the four main compounds of Z. nitidum in cardiovascular diseases, their impact on the electrical activity of cardiomyocytes, and the underlying mechanism of their anti-inflammatory effects. We selected the four compounds from Z. nitidum with a high concentration and specific biological activity: nitidine chloride (NC), chelerythrine chloride (CHE), magnoflorine chloride (MAG), and hesperidin (HE). A proteomic analysis was conducted on the myocardial tissues of beagle dogs following the administration of NC to investigate the role of NC in vivo and the associated biological processes. A bioinformatic analysis was used to predict the in vivo biological processes that MAG, CHE, and HE were involved in. Molecular docking was used to simulate the binding between compounds and their targets. The effect of the compounds on ion channels in cardiomyocytes was evaluated through a patch clamp experiment. Organ-on-a-chip (OOC) technology was developed to mimic the physiological conditions of the heart in vivo. Proteomic and bioinformatic analyses demonstrated that the four compounds of Z. nitidum are extensively involved in various cardiovascular-related biological pathways. The findings from the patch clamp experiments indicate that NC, CHE, MAG, and HE elicit a distinct activation or inhibition of the IK1 and ICa-L in cardiomyocytes. Finally, the anti-inflammatory effects of the compounds on cardiomyocytes were verified using OOC technology. NC, CHE, MAG, and HE demonstrate anti-inflammatory effects through their specific interactions with prostaglandin-endoperoxide synthase 2 (PTGS2) and significantly influence ion channels in cardiomyocytes. Our study provides a foundation for utilizing NC, CHE, MAG, and HE in the treatment of cardiovascular diseases.
Investigation on the Combined Effect of Hydroxypropyl Beta-Cyclodextrin (HPβCD) and Polysorbate in Monoclonal Antibody Formulation
Authors: Huang Jiayi , Hong Shiqi , Goh Lucas Yuan Hao , Zhang Hailong , Peng Tao , Chow Keat Theng , Gokhale Rajeev , Tuliani Vinod
Published: April 2024

Monoclonal antibodies require careful formulation due to their inherent stability limitations. Polysorbates are commonly used to stabilize mAbs, but they are prone to degradation, which results in unwanted impurities. KLEPTOSE® HPβCD (hydroxypropyl beta-cyclodextrin) has functioned as a stable stabilizer for protein formulations in our previous research. The current study investigates the collaborative impact of combining polysorbates and HPβCD as excipients in protein formulations. The introduction of HPβCD in formulations showed it considerably reduced aggregation in two model proteins, bevacizumab and ipilimumab, following exposure to various stress conditions. The diffusion interaction parameter revealed a reduction in protein–protein interactions by HPβCD. In bevacizumab formulations, the subvisible particle counts per 0.4 mL of samples in commercial formulations vs. formulations containing both HPβCD and polysorbates subjected to distinct stressors were as follows: agitation, 87,308 particles vs. 15,350 particles; light, 25,492 particles vs. 6765 particles; and heat, 1775 particles vs. 460 particles. Isothermal titration calorimetry (ITC) measurement indicated a weak interaction between PS 80 and HPβCD, with a KD value of 74.7 ± 7.5 µM and binding sites of 5 × 10–3. Surface tension measurements illustrated that HPβCD enhanced the surface activity of polysorbates. The study suggests that combining these excipients can improve mAb stability in formulations, offering an alternative for the biopharmaceutical industry.
Development of Mechanistic In Vitro–In Vivo Extrapolation to Support Bioequivalence Assessment of Long-Acting Injectables
Authors: Amaral Silva Daniela , Le Merdy Maxime , Alam Khondoker Dedarul , Wang Yan , Bao Quanying , Malavia Nilesh , Burgess Diane , Lukacova Viera
Published: April 2024

Long-acting injectable (LAI) formulations provide sustained drug release over an extended period ranging from weeks to several months to improve efficacy, safety, and compliance. Nevertheless, many challenges arise in the development and regulatory assessment of LAI drug products due to a limited understanding of the tissue response to injected particles (e.g., inflammation) impacting in vivo performance. Mechanism-based in silico methods may support the understanding of LAI–physiology interactions. The objectives of this study were as follows: (1) to use a mechanistic modeling approach to delineate the in vivo performance of DepoSubQ Provera® and formulation variants in preclinical species; (2) to predict human exposure based on the knowledge gained from the animal model. The PBPK model evaluated different elements involved in LAI administration and showed that (1) the effective in vivo particle size is potentially larger than the measured in vitro particle size, which could be due to particle aggregation at the injection site, and (2) local inflammation is a key process at the injection site that results in a transient increase in depot volume. This work highlights how a mechanistic modeling approach can identify critical physiological events and product attributes that may affect the in vivo performance of LAIs.
Non-Thermal Plasma (NTP) Treatment of Alfalfa Seeds in Different Voltage Conditions Leads to Both Positive and Inhibitory Outcomes Related to Sprout Growth and Nutraceutical Properties
Authors: Motrescu Iuliana , Lungoci Constantin , Calistru Anca Elena , Luchian Camelia Elena , Gocan Tincuta Marta , Rimbu Cristina Mihaela , Bulgariu Emilian , Ciolan Mihai Alexandru , Jitareanu Gerard
Published: April 2024

Non-thermal plasma (NTP) has proven to be a green method in the agricultural field for the stimulation of germination, growth, and production of nutraceutical compounds in some cases. However, the process is far from being fully understood and depends on the targeted plant species and the NTP used. In this work, we focus on the production of alfalfa sprouts from NTP-treated seeds under different voltage conditions. A flexible electrode configuration was used to produce the NTP, which can also be placed on packages for in-package treatments. The surface of the seeds was analyzed, indicating that the microstructure was strongly affected by NTP treatment. Biometric measurements evidenced the possibility of stimulating the sprout growth in some conditions by up to 50% compared to the sprouts obtained from untreated seeds. Biochemical traits for the sprouts obtained in different processing conditions were also studied, such as the concentrations of chlorophyll pigments, flavonoids and polyphenols, and antioxidant activity. Most NTP treatments led to inhibitory effects, proving the strong dependence between NTP treatment and targeted plant species.
Combined Application of Biochar and Plant Growth-Promoting Rhizobacteria Improves Heavy Metal and Drought Stress Tolerance in Zea mays
Authors: Anbuganesan Vadivel , Vishnupradeep Ramasamy , Bruno L. Benedict , Sharmila Krishnan , Freitas Helena , Rajkumar Mani
Published: April 2024

Plants are often exposed to multiple stresses, including heavy metals (HM) and drought, which limit the plant growth and productivity. Though biochar or plant growth-promoting rhizobacteria (PGPR) have been widely used for alleviating HM or drought stress in plants, the study of the effects of combined treatment with biochar and PGPR under simultaneous HM and drought stress is limited. This study investigated individual and combined effects of groundnut shell biochar (GS-BC) and PGPR Bacillus pseudomycoides strain ARN7 on Zea mays growth, physiology, and HM accumulation, along with their impact on soil enzymes under HM (Ni and Zn), drought, or HM+drought stress. It was observed that even under HM+drought stress, Z. mays growth, total chlorophyll, proteins, phenolics, and relative water contents were increased in response to combined GS-BC and ARN7 treatment. Furthermore, the combined treatment positively influenced plant superoxide dismutase, ascorbate peroxidase, and catalase activities, while reducing electrolyte leakage and phenolics, malondialdehyde, and proline under HM, drought, or HM+drought stress. Interestingly, the combined GS-BC and ARN7 treatment decreased HM accumulation and the bioaccumulation factor in Z. mays, highlighting that the combined treatment is suitable for improving HM phytostabilization. Additionally, GS-BC increased soil enzymatic activities and ARN7 colonization irrespective of HM and drought stress. As far as we know, this study is the first to illustrate that combined biochar and PGPR treatment could lessen the adverse effects of both HM and drought, suggesting that such treatment can be used in water-deficient HM-contaminated areas to improve plant growth and reduce HM accumulation in plants.
Last-Century Forest Dynamics in a Highland Pyrenean National Park and Implications for Conservation
Authors: Rull Valentí , Blasco Arnau , Sigro Javier , Vegas-Vilarrúbia Teresa
Published: April 2024

Ecological records from before and after the creation of natural parks are valuable for informing conservation and management but are often unavailable. High-resolution paleoecological studies may bridge the gap and provide the required information. This paper presents a 20th-century subdecadal reconstruction of vegetation and landscape dynamics in a national park of the Pyrenean highlands. The park lands had traditionally been used for cultivation, extensive grazing, forest exploitation, and hydroelectricity generation following the damming of numerous glacial lakes. A significant finding is that forests have dominated the landscape, with negligible changes in composition, and only experienced fluctuations in forest cover, influenced by both climatic and anthropogenic factors. The creation of the park (1955) and the initial restrictions on forest exploitation did not significantly affect vegetation cover or composition. Major forest expansion did not occur until several decades later, 1980, when the park was enlarged and forest exploitation was further restricted. This expansion peaked in the 1990s, coinciding with a warming trend and a decrease in fire incidence, before declining due to warmer and drier climates. This decline was coeval with the ongoing global forest dieback and may be exacerbated by the predicted global warming in this century, which could also increase fire incidence due to dead-wood accumulation. Currently, the main threats are global warming/drying, fire, and tourism intensification. Similar high-resolution paleoecological records in protected areas are globally scarce and would be capable in providing the long-term ecological scope required to properly understand forest dynamics and optimize conservation measures.
Interaction of Soybean (Glycine max (L.) Merr.) Class II ACBPs with MPK2 and SAPK2 Kinases: New Insights into the Regulatory Mechanisms of Plant ACBPs
Authors: Moradi Atieh , Lung Shiu-Cheung , Chye Mee-Len
Published: April 2024

Plant acyl-CoA-binding proteins (ACBPs) function in plant development and stress responses, with some ACBPs interacting with protein partners. This study tested the interaction between two Class II GmACBPs (Glycine max ACBPs) and seven kinases, using yeast two-hybrid (Y2H) assays and bimolecular fluorescence complementation (BiFC). The results revealed that both GmACBP3.1 and GmACBP4.1 interact with two soybean kinases, a mitogen-activated protein kinase MPK2, and a serine/threonine-protein kinase SAPK2, highlighting the significance of the ankyrin-repeat (ANK) domain in facilitating protein–protein interactions. Moreover, an in vitro kinase assay and subsequent Phos-tag SDS-PAGE determined that GmMPK2 and GmSAPK2 possess the ability to phosphorylate Class II GmACBPs. Additionally, the kinase-specific phosphosites for Class II GmACBPs were predicted using databases. The HDOCK server was also utilized to predict the binding models of Class II GmACBPs with these two kinases, and the results indicated that the affected residues were located in the ANK region of Class II GmACBPs in both docking models, aligning with the findings of the Y2H and BiFC experiments. This is the first report describing the interaction between Class II GmACBPs and kinases, suggesting that Class II GmACBPs have potential as phospho-proteins that impact signaling pathways.
Enhancing Mechanical and Thermal Properties of 3D-Printed Samples Using Mica-Epoxy Acrylate Resin Composites—Via Digital Light Processing (DLP)
Authors: Senthooran Velmurugan , Weng Zixiang , Wu Lixin
Published: April 2024

Digital light processing (DLP) techniques are widely employed in various engineering and design fields, particularly additive manufacturing. Acrylate resins utilized in DLP processes are well known for their versatility, which enables the production of defect-free 3D-printed products with excellent mechanical properties. This study aims to improve the mechanical and thermal properties of 3D-printed samples by incorporating mica as an inorganic filler at different concentrations (5%, 10%, and 15%) and optimizing the dispersion by adding a KH570 silane coupling agent. In this study, mica was introduced as a filler and combined with epoxy acrylate resin to fabricate a 3D-printed sample. Varying concentrations of mica (5%, 10%, and 15% w/w) were mixed with the epoxy acrylate resin at a concentration of 10%, demonstrating a tensile strength increase of 85% and a flexural strength increase of 132%. Additionally, thermal characteristics were analyzed using thermogravimetric analysis (TGA), and successful morphological investigations were conducted using scanning electron microscopy (SEM). Digital light-processing technology was selected for its printing accuracy and cost-effectiveness. The results encompass comprehensive studies of the mechanical, thermal, and morphological aspects that contribute to the advancement of additive manufacturing technology.
Cure Kinetics and Thermal Decomposition Behavior of Novel Phenylacetylene-Capped Polyimide Resins
Authors: Xiong Xuhai , Guan Hongyu , Li Baiyu , Yang Shuai , Li Wenqiang , Ren Rong , Wang Jing , Chen Ping
Published: April 2024

Based on a novel phenylacetylene capped polyimide (PI) with unique high-temperature resistance, its curing kinetics and thermal decomposition behavior were investigated. The curing mechanism and kinetics were studied by differential scanning calorimetry (DSC), and the activation energy (Ea) and pre-exponential factor (A) of the curing reaction were calculated based on the Kissinger equation, Ozawa equation, and Crane equation. According to the curve of conversion rate changing with temperature, the relationship between the dynamic reaction Ea and conversion rate (α) was calculated by the Friedman equation, Starink equation, and Ozawa–Flynn–Wall (O-F-W) equation, and the reaction Ea in different stages was compared with the results of molecular dynamics. Thermogravimetric analysis (TGA) and a scanning electron microscope (SEM) were used to analyze the thermal decomposition behavior of PI resins before and after curing. Temperatures at 5% and 20% mass loss (T5%, T20%), peak decomposition temperature (Tmax), residual carbon rate (RW), and integral process decomposition temperature (IPDT) were used to compare the thermal stability of PI resins and cured PI resins. The results display that the cured PI has excellent thermal stability. The Ea of the thermal decomposition reaction was calculated by the Coats–Redfern method, and the thermal decomposition behavior was analyzed. The thermal decomposition reaction of PI resins at different temperatures was simulated by molecular dynamics, the initial thermal decomposition reaction was studied, and the pyrolysis mechanism was analyzed more comprehensively and intuitively.
Evaluation of the Effectiveness of Geogrids Manufactured from Recycled Plastics for Slope Stabilization—A Case Study
Authors: Vicuña Lenin , Jaramillo-Fierro Ximena , Cuenca Paúl Eduardo , Godoy-Paucar Brenda , Inga-Lafebre Jorge Daniel , Chavez Torres Jose Luis , García Juan Fernando , Guaya Diana , Febres Juan Diego
Published: April 2024

This study aimed to investigate the sustainable use of recycled plastics, specifically polypropylene (PP) and high-density polyethylene (HDPE), in the manufacture of geogrids for geotechnical and civil engineering applications. Plastics were collected from a recycling center, specifically targeting containers used for food, cleaning products, and other domestic packaging items. These plastics were sorted according to the Möbius triangle classification system, with HDPE (#2) and PP (#5) being the primary categories of interest. The research methodologically evaluates the mechanical properties of PP/HDPE (0/100, 25/75, 50/50, 75/25 and 100/0% w/w) composites through tensile and flexural tests, exploring various compositions and configurations of geogrids. The results highlight the superiority of pure recycled HDPE processed into 1.3 mm thick laminated yarns and hot air welded for 20 to 30 s, exhibiting a deformation exceeding 60% in comparison to the PP/HDPE composites. Through SolidWorks® Simulation, it was shown that the adoption of a trigonal geogrid geometry optimizes force distribution and tensile strength, significantly improving slope stabilization efficiency. Based on the results obtained, a laboratory-scale prototype geogrid was developed using an extrusion process. The results underscore the importance of careful composite design and yarn configuration selection to achieve the desired mechanical properties and performance in geogrid applications. It emphasizes the potential of recycled plastics as a viable and environmentally friendly solution for stabilizing slopes, contributing to the reduction in plastic waste and promoting sustainable construction practices.
Changes in Soil Microbial Parameters after Herbicide Application in Soils under Conventional Tillage and Non-Tillage
Authors: Douibi Marwa , Carpio María José , Rodríguez-Cruz María Sonia , Sánchez-Martín María J. , Marín-Benito Jesús M.
Published: April 2024

This study evaluated the changes in microbial activity in the course of time following the joint application of the herbicides S-metolachlor, foramsulfuron, and thiencarbazone-methyl to two soils (S1 and S2) under conventional tillage (CT) and non-tillage (NT) management in field conditions. The biochemical parameters of soil respiration (RES), dehydrogenase activity (DHA), microbial biomass (BIO), and the phospholipid fatty acid (PLFA) profile were determined at 1, 34, and 153 days during herbicide dissipation. In the absence of herbicides, all microbial activity was higher under NT than CT conditions, with higher or similar mean values for S1 compared to S2. A continuous decrease was detected for RES, while DHA and BIO recovered over time. In the presence of herbicides, a greater decrease in all microbial activity was detected, although the changes followed a similar trend to the one recorded without herbicides. In general, a greater decrease was observed in S1 than in S2, possibly due to the higher adsorption and/or lower bioavailability of herbicides in this soil with a higher organic carbon content. The decrease was also greater under CT conditions than under NT conditions because the herbicides can be intercepted by the mulch, with less reaching the soil. These changes involved evolution of the structure of the microbial community.
Two Types of Philosophy of Religion: Neutral Cognition versus Lived Experience
Authors: Rivera Joseph
Published: April 2024

As a discipline taught and debated in universities and seminaries, philosophy of religion has traditionally been understood as a form of apologetics: that is, it pursues with concentrated effort what proof for the existence of God the human mind can mobilize with recourse strictly to reason and logic [...]
An Assessment of Socio-Economic Status of Women on Family Farms: Slovenian Case Study
Authors: Prišenk Jernej , Vesenjak Urška , Rozman Črtomir , Turk Jernej , Pažek Karmen
Published: April 2024

The question of gender equality is increasingly being raised today and is present at all levels of society. The topicality of the issue on farms is particularly evident, due to the particular inheritance processes on farms, the clear division of labour, and intergenerational cooperation that characterise the agricultural sector. In this research, a multi-criteria model (DEX-SOCIAL) was developed to understand the broader aspect of rural sociology and the issue of women’s status on the farm. The paper discusses the status of women on a farm and assesses their social and economic situation. The methodology includes an online questionnaire in which women in the Eastern and Western Cohesion Regions participated, as well as other farm members and owners. Subsequently, the questions were transformed for the requirements of the assessment model, which assessed the life prospects of women on farms in both the Eastern and Western Cohesion Regions who were aged both over and under 40 years (criteria for “young successor”). The results of the study show that there is a clear difference in the qualitative assessment of women’s socio-economic position in relation to the East–West cohesion region. The social position of women does not differ according to age structure. The conclusions of the study also present broader applications of the results in the field of rural development and rural sociology.