bluuebunny/crossref_metadata_2025 huggingface dataset:
PROMOTION OF FISHING GOODS AT THE UKRAINIAN MARKET
Authors: Mohylova Anastasiia , Ponomarenko Daniil
Published: August 2021

Due to the constant development of innovations related to the ways of promoting goods, they need to be constantly monitored and analyzed in order to use them to improve commercial success. The main mistake, in our opinion, is a little attention to Internet promotion, because this area is becoming more popular every year, and with the conditions of a global pandemic comes to the fore. The promotion of goods of the “Fanatik” chain of branded stores at the market of fishing goods has been studied. TM “Fanatik” is one of the most successful companies of fishing goods in Ukraine, the target audience of the company is ordinary fishermen. In order to find out the reasons for low sales, ABC-XYZ conducted an analysis of the range of “Fanatik” chain stores. It is concluded that in almost all cities where there are “Fanatik” stores and which have been analyzed, the results are the same. The components of the marketing complex of TM “Fanatik” and the complex of promotion of goods of TM “Fanatik” are analyzed. The company uses almost the entire range of promotion tools, namely: direct marketing, personal sales, advertising, especially closely uses public relations and telemarketing, because it is thanks to them that it is possible to maintain a stable profit. But very little attention is paid to promotion through the online store, as a result of which the site began to lose visitors, and competitors gain consumers of the company. According to the results of the SWOT-analysis, it was determined that the main weaknesses of the company are the low level of service and opening hours of stores, and the main strengths are that the leader is Yuri Petrash himself and the lowest price on the market. The Ansoff matrix was used to determine the new strategy for the company. A new complex of promotion of “Fanatik” TM products has been developed and prospects for its application have been determined. It will be possible not only to improve the existing components of the old promotion complex, but also to use new, modern promotion components, which will significantly increase not only the profit from the online store, but also the overall annual profit of the company.
THE TALENT POOL FORMATION AS A TOOL OF MOTIVATION MANAGEMENT
Authors: Hryshchenko Diana , Kaidanovych Vladyslav
Published: August 2021

The current situation in the labor market and wholesale and retail trade changes in a straight line with the economic, political, social sphere. And it assumes that the changes are non-reversible. At this stage of economic development, enterprises of trade industry act as a kind of concentrator of human resource. Enterprises are responsible for qualitative selection and recruitment of personnel, for its development. Combination of these factors makes enterprise management allocate more and more financial resources for creation of qualitative and quantitative base of human resource. It is the personnel of the enterprise that actualizes the presence and formation of the talent pool at the enterprise. In the article essence of a scientific category “talent pool” and its formation is investigated This theme is actual at the moment, as, the talent pool accumulates perspective and professionally developed employees, that in turn positively influences the enterprise. Especially actual are development and introduction in practice of the domestic enterprises of such talent pool, which primary goal is maintenance of steady and the most effective work of the enterprise which in the near future can provide high level of development both the enterprise, and its personnel, and also to have high level of motivation not only reservists and potential reservists. Thus, authors had been allocated a certain problem at formation of the talent pool, but, contrary to it a number of advantages of formation of the talent pool at the enterprise from the side of formation of the talent pool and from the side of a motivating factor were allocated. On the side of the motivational factor, the main known theories of motivation were studied and analyzed how each theory reveals the essence of the talent pool at the enterprise as an integral element of the personnel strategy of the enterprise. For the purpose of improvement of formation of talent pool, foreign experience was studied and on its basis the author’s method of formation of talent pool on the principle of division of employees into “career” and “non-career” for a particular enterprise taking into account its specific features were developed and offered. The prospect of further research and development of this topic is to identify new methods of forming the talent pool, which will have the highest degree of efficiency and improvement of existing methods.
FEATURES OF THE FORMATION AND VALUATION OF INSURANCE RESERVES
Authors: Kompaniets Maxim , Kysilyova Inna
Published: August 2021

The purpose of the paper is research of practice of making insurance reserves of the insurance companies in Ukraine and summarizes the ways of improvement of methods for their calculation with the purpose of increasing management efficiency of an organization. The article addresses the characteristics and economic nature of certain types of technical reserves of insurance organizations in particular the unencumbered premiums reserve, the loss reserve and the catastrophe reserve, and the characteristics of their formation. Major methods for calculating the reserve of unencumbered premiums reviewed and recommended adjustment to method 1/36 , and use of the reserve calculation method of unencumbered premiums, which takes into account inflation ratio. The method of calculation and formation of the loss reserve is considered as well as the characteristics of the reserve for past but undeclared losses and reserves for asserted but unresolved losses. The system of indicators of sufficiency of insurance reserves of insurance organizations was analyzed; the calculation formulae and recommended values are given. Insurance reserves sufficiency ratios refers to the status of insurance reserves and determine the adequacy of insurance reserves to the risks taken into insurance. Sufficiency ratio (based on premiums) and sufficiency ratio (based on payments) determine, respectively, the upper and lower limits of insurance reserves. For conducting research and substantiation of relevant conclusions, the indicators of dynamics and structure of insurance reserves of insurance company JSC IC “INGO” are analyzed. Sufficiency ratios for insurance reserves of JSC IC “INGO” are also calculated and Evaluation of the company’s insurance reserves has been performed. The results of the study can be applied by the heads of the financial divisions of insurance companies for the development of tactical and strategic decisions that allows to yield optimal condition of insurance reserves and their reliable valuation of insurance company and to perform the quick analysis of the state of insurance reserves of insurance company.
ESSENCE AND STRUCTURE OF PUBLIC FINANCE: THEORETICAL ASPECT
Authors: Prutska Olena
Published: August 2021

The article is devoted to the theoretical aspects of the functioning of public finances. In Ukraine, public finances account for more than 40% of gross domestic product. Public finances provide funding for state functions, including territorial integrity, law and order, health care, education funding, pensions, social protection, infrastructure development, environmental protection, and more. The formation of an effective system of public finance has become one of the key tasks of the current stage of development of the Ukrainian economy. Its solution requires the creation of a holistic concept of formation, development and improvement of the public finance system of Ukraine. In the domestic economic literature in the study of the problems of state finance, its revenues and expenditures, the phrase “state finance” was common. The term “public finance” is considered by experts to be a relatively young category of Ukrainian science, the active application of which began with the implementation of public administration reform. The purpose of the article is to clarify the nature and composition of public finances as a necessary resource for the functioning of the state, local governments, public needs of society. The article analyzes the definitions of “public finance”. It is concluded that Ukrainian economists have not yet agreed on the essence and components of the public finance system. The author presents arguments in favor of an expanded interpretation of this category, proposed his own definition of public finance. It is proposed to include the budget system, extra-budgetary trust funds, and finances of state and communal enterprises, financial and non-financial public corporations, enterprises within the framework of public-private partnership to public finances. Such an expanded interpretation of the structure of public finances will clarify the mechanism of their management, develop a system of incentives, forms of influence and control, and in particular by drawing more attention to the activities of state and municipal enterprises, develop clear criteria for quantitative parameters of public sector enterprises and their financial indicators.
DEVELOPMENT OF THE INFORMATION SOCIETY IN UKRAINE – THE BASIS OF SUCCESSFUL EUROPEAN INTEGRATION: STRATEGY, TASKS, PRINCIPLES
Authors: Tur Oleksandr , Prigara Iryna , Novikova Inna
Published: August 2021

The active development of the information society in the world and in Ukraine significantly raises the issue of the development of electronic interaction between the state, business and society. The development of electronic interaction in Ukraine is taking place through the prism of public administration reform, which cannot be imagined without the participation of computer and information technologies. Direct development of the information society is impossible to imagine without a developed e-government system in Ukraine. In the light of the signed Association Agreement between Ukraine and the European Union, the problem of developing and implementing joint strategic projects aimed at the systematic development of e-government in Ukraine in accordance with European standards and requirements becomes urgent. The presented study outlines the main stages of development of the information society in Ukraine. The peculiarities of the development of the information society in Ukraine before 2014 (the year of signing the Association Agreement between Ukraine and the EU) and after 2014 are studied. A study of the peculiarities of the work of the State Agency for e-Government of Ukraine and the Ministry of Digital Transformation of Ukraine, which worked on the development of the information society in Ukraine after 2014. The achievements of the Ministry of Digital Transformation of Ukraine over the years of its activity in the field of information society development in Ukraine are determined. The main strategic goals of the ministry for the short term are analyzed. A study of the peculiarities of the work of the Ministry of Digital Transformation of Ukraine in the planning and implementation of digital transformation projects. Based on the analysis, the main shortcomings of the Ministry of Digital Transformation of Ukraine, which hinder the development of the information society in Ukraine and European integration processes, are highlighted. The authors propose a system of principles guided by the Ministry of Digital Transformation of Ukraine in the development of the information society in Ukraine.
MODERN LENDING OF AGRICULTURE
Authors: Zhuravleva Anastasiya , Batrakova Tatiana
Published: August 2021

The purpose of the article is to study the main problems of lending to agro-industrial enterprises with state support in market relations and propose measures to implement a new mechanism of subsidized and compensatory budget support for agriculture, aimed at its effective functioning. Historically, Ukraine is an agro-industrial country with rich natural resources and opportunities. However, to make a decent profit, the agro-industrial complex needs regular cash infusions and complete modernization. Given these factors, farmers in Ukraine are increasingly resorting to such an ancillary method as a loan for agricultural development. The long history of the Ukrainian economy, multiplied by current trends in crop production and animal husbandry, forces banks to consider lending to agriculture as a promising profitable business, which can and should be lent. The urgency of the study is due to the growing need to address one of the priority sectors of the economy-agriculture, by increasing the role and availability of credit for farmers, improving lending conditions, improving the efficiency of monetary institutions lending to agricultural enterprises, as well as development forms of their support. Problems of improving the lending system, investment activity of enterprises, lending mechanism and state regulation in the field of agriculture were covered in the works of a number of scientists and practitioners: G. Bezorna, H. Borkhunov, I. Buzdalov, A. Gataulin, A. Golovanov, A. Gordeeva, L. Kolichev, K. Koroleva, A. Kupavykh, D. Murzin, E. Mezentsev, A. Ponomareva, E. Sagaidak, A. Sidoruk, Y. Trushin, D. Epstein and some others. In the foreign economic literature, the main attention is paid to the forms of state support for agricultural producers, assessment of the impact of subsidies in terms of efficient use of public funds and the functioning of agricultural banks, but at the level of microfinance. These problems are reflected in the works of foreign authors: N. Gow, K. Hoff, M. Zeller, A. Sarris, J. Swinnen, J. Stiglitz, J. Yaron, M. Schreiner, Le Min Tu, and others. The changing conditions of the market environment pose new challenges to the subjects of credit relations, and then there are problems of agricultural lending in the changing conditions of the country’s economy, which need to be addressed. Formulation of the goals of the article (task statement). The purpose of the article is to generalize the theory and practice of agricultural lending when there are new economic relations, financial and credit opportunities of the Ukrainian economy.
PUBLICITY OF SHARE PRODUCTS – FEATURES OF FUNCTIONS IN UKRAINE
Authors: Zaitseva Liudmyla
Published: August 2021

The movement of the economy on the path of efficiency, rationalization and constant renewal, through the creation of an innovative environment that destroys traditional structures and opens opportunities for transformation is achieved through the activities of public companies that are subjects of the stock market. The presented article is devoted to substantiation of theoretical positions and definition of features of activity of public companies on the basis of experience of the countries of the European Union. The activities of public companies are regulated by law and related to the stock market. The key theses of the legislative base of Ukraine concerning the activity of joint-stock companies are given. Emphasis is placed on a clear criterion for classifying a joint-stock company as a public one, due to cooperation with the stock market. The peculiarities of the functioning of modern foreign public companies, including in comparison with private campaigns, are given. The results of the analysis of the dynamics of the number of public companies indicate the low readiness of Ukrainian business for transparent activities, and the underdevelopment of the Ukrainian stock market limits the prospects for choosing sources of increasing the market value of public companies in the future. The publicity of companies in Ukraine is questionable due to the lack of public offerings by issuers of shares in Ukraine. These facts point to the problems associated with increasing the level of capitalization of the company by increasing the market value of capital and the formation of effective sources of financing by expanding the range of financial instruments and resources. The peculiarities of this form of entrepreneurship (infinity of stakeholders, openness of information, legality of actions, sustainability of business models, transparency of decisions) due to increased investment attractiveness and liquidity of assets for investors, which is achieved by ensuring openness, transparency and social responsibility of public companies.
ECONOMETRIC MODEL OF THE TOURIST ENTERPRISES ACTIVITY ASSESTMENT
Authors: Liashenko Oksana , Kondratska Liliya , Demianiuk Olga
Published: August 2021

The work aims to develop an author's approach to modelling the performance of tourism enterprises using modern mathematical methods of multifactor modelling based on empirical data. To achieve this goal, the following tasks were set and solved: to generalize theoretical developments and practical approaches to evaluating the effectiveness of tourism enterprises at the regional and national levels; substantiate the methodological basis and choice of econometric tools for modelling the evaluation of the effectiveness of tourism enterprises, taking into account the available statistical base; to develop an econometric model for evaluating the effectiveness of tourism enterprises that would allow determining a system of indicators for assessing the impact of the effectiveness of tourism enterprises on the economic development of territories; to develop recommendations for the application of the author's model in the substantiation and management decisions in the process of forming the foundations of regional and national economic policy.
The author's approach to assessing the impact of tourism enterprises on the processes of economic development of the regions is proposed in the article. In the process of econometric modelling, the method of analysis of canonical correlations was used, which allowed obtaining an analytical view of empirical relationships of sets of performance indicators of tourism enterprises and indicators of economic development of regions, which, in turn, allows to obtain a formalized interaction of tourism enterprises and region. to obtain a realistic estimate of the contribution of tourism enterprises to GRP generation.
The canonical analysis of the interaction of indicators reflecting the economic performance of tourism enterprises in the regions of Ukraine and indicators of economic development of the regions showed that the most priority areas of tourism enterprises in the regions of Ukraine that contribute to the development of regions, territories and increase employment are revenues from tourism services in the form of sale of tourist vouchers to citizens of Ukraine abroad for the purpose of specialized tourism, leisure, recreation and children's tourism.
The growth of the share of tourist services both in the structure of gross regional product and in the structure of consumption in the world should stimulate domestic tourist enterprises to work towards increasing those indicators of tourist activity that would increase their profitability and competitiveness in the distribution of tourist flows. with the state, national tourist facilities and tourist infrastructure, especially in regions with sufficient tourist potential and low level of its use, to ensure a high level of quality of tourist services in accordance with international standards, to develop tourist activities within Ukraine.
Gælunöfn í ættartölusafnriti
Authors: Sigmundsson Svavar
Published: August 2021

Greinin fjallar um íslensk gælunöfn í Ættartölusafnriti Þórðar Jónssonar frá Hítardal (1645-1669).
Stafræn gerð Blöndalsorðabókar
Authors: Jónsdóttir Halldóra , Úlfarsdóttir Þórdís
Published: August 2021

The digitisation of a 100-year-old dictionary, Íslensk-dönsk orðabók, was completed in 2020. With Icelandic as a source language and Danish as a target language, the work is one of the most comprehensive dictionaries ever published in Iceland. It is now available on the internet, free of charge (see blondal.arnastofnun.is). The authors, Sigfús and Björg Þ. Blöndal, spent two decades compiling the dictionary, and to this day it remains an influential work for Icelandic lexicography, being an important authority on the Icelandic language as it was spoken and written in the early 20th century.
SCIENCE PARADIGM AND PUBLISHING ADEQUACY: WHAT WE WOULD LIKE TO READ IN SCIENTIFIC JOURNALS
Authors: Lakhvich Todar
Published: August 2021

A few days ago I had an interesting discussion with my master student who recently had entered our university after his graduation as a bachelor from an Arab university. As usual, I gave him few questions for analysis through reviewing scientific Journals and monographs. It was the easiest way to check what his grades in Diploma are worth of and skills in working on a thesis. In all the cases the first chapter of a thesis is to comprise a scientific analysis of the focal problem and therefore it resembles be an introduction to experimental research, in our case in the field of applied Biochemistry.
After years of being a university professor I’m rather sceptical about students’ ability to offer at the first attempt a proper scientific review that would meet all the requirements (strangely enough, it’s a matter of consideration for some proposals for a scientific Journal). Still I’m an acting professor, appointed as the supervisor for the foreign student and should teach him skills of a scientist. It is just a normal practice. I am used to teach and like to do it, and accept the idea the first attempt barely gets you the final result; anyway, I am there to help to overcome the difficulties (which is an approach in Science education that gives students good experience to enable them to work further on more independently. I am used to correct and edit, to teach and explain, to discuss findings and help to do work in a proper way. That’s just all right, that’s the process of learning. But in that case, I was absolutely disappointed.
INTEGRATING VISUALIZATIONS IN SCIENCE TEACHING: TEACHERS’ DIFFICULTIES AND PEDAGOGICAL APPROACHES
Authors: Ferreira Celeste , Baptista Mónica , Arroio Agnaldo
Published: August 2021

This study is a qualitative research where it tries to finding out and analyze the teacher’s pedagogical approaches and difficulties to introduce visualizations in science classroom. The use of visualizations in classroom has increased substantially, especially since the development of Information and Communication Technology (ICT). Fourteen in-service teachers were investigated across a teachers’ training course to seek their difficulties and pedagogical approaches for integrating these tools in teaching and learning sequences (TLS). During the training teachers were invited to build in group (five groups) teaching learning sequences about some science content using visualizations. The methodology is qualitative and a case study design was adopted. It was analysed the TLS made by them and the audiovisual record of their oral communications to the class. It was also analyzed the final semi-structured interview with these teachers’ groups and a final report about the TLS application in classroom. These teachers present mainly pedagogical difficulties in order to select the suitable resource and to realize the full potentialities of these resources and sometimes the role of the teacher towards students and resources in the classroom. The technical barriers were the lack of school computers and appropriated software. The results suggest that these teachers are using mostly constructivist approaches to incorporate the visualizations in classroom trying to take advantage of the interactivity that some of these tools allow. Two groups fall more in transmission view, using this resources mostly to present information and the rest tried to use this resources to build inquiry tasks. They chose above of all multimedia tools to enhance particular concepts and skills and sometimes to innovate presentations. Some difficulties related to pedagogical approaches and technical barriers to use these resources are discussed.
Key words: approaches, difficulties, visualizations, teacher pedagogical education.
Implementation of Islamic Values in The Employees Recruitment Process
Authors: Yuli Sri Budi Cantika , Sari Novi Primita
Published: August 2021

This study aimed at determining the implementation of Islamic values in the recruitment process (Case Study at University of Muhammadiyah Malang-UMM). This research applied qualitative method through a phenomenological approach. The informants of this study were (1) University Leaders; (2) Employees. Further, the data collected were analyzed using qualitative data analysis techniques, namely: Data Reduction, Data Presentation, and Conclusion Drawing. The results of this study indicated that the recruitment process at UMM: 1) in accordance with Islamic values sourced from the Qur'an and Hadith; 2) UMM leaders have accomplished the mandate that was showed in selecting employees and establishing the law fairly according to Q.S. An Nisa [4]: 58 and Q.S. Al Maidah [5]: 8. UMM Leaders appoint the most worthy and appropriate employees as in Q.S. An Nisaa [4]: 59; 4) UMM leaders choose employees of the highest quality selectively in accordance with the words of the Prophet Muhammad in HR. Baihaqi; 5) UMM Leaders choose capable and trustworthy employees according to Q.S. Al Anfaal [8]: 27.
ENGLISH AS A FOREIGN LANGUAGE FOR STUDENTS OF STAKN KUPANG: A STUDY OF LEARNERS’ NEEDS
Authors: Sine Junaity Soften , A. Bekuliu Desty , Loak Mardolitha
Published: August 2021

The process in identifying the situation analysis of a target language is called Needs Analysis or Needs Assessment (NA). To be effective and efficient in learning process in English classroom at STAKN Kupang, this analysis is considered important. NA is divided into two parts of analysis namely present situation analysis and target situation analysis. A quantitative approach was used in this research with a survey method. The findings show: 1) In general, the English proficiency of the students of Study Program Christian Religious Education in academic year 2018-2019 was beginner (basic); 2) There were several reasons why they stopped learning English such as the materials used were not applicable on daily basis, their teachers did not use good teaching method, or the materials was not interesting and challenging; 3) Students thought that learning grammar and the differences between Bahasa Indonesia and English is important to be covered in textbooks; 4) Students preferred their teachers to explain the grammatical theories before giving them practice activities; 5) Even though students admitted that English is a difficult language, they agreed that English is very important to be mastered and it has become a language widely used for international occasions.
Aplikasi Rekomendasi Resep Masakan Khas Indonesia bagian Timur Menggunakan Metode Collaboration Collective Intelligence dan Slope One
Authors: Soekarta Rendra , Modok Mersi
Published: August 2021

Resep makanan penting dalam komposisi hidangan, dan juga tidak hanya secara fisik tetapi beberapa dibutuhkan digital. Jadi pengguna tidak perlu menyimpan buku resep yang telah dibeli untuk menemukan resep. Dari situs web yang memberikan rekomendasi untuk digital panduan resep adalah dapurSiapsaji. Aplikasi ini membantu pengguna untuk mencari resep hanya dengan memasukkan bahan dari makanan yang dimiliki oleh pengguna. Dan itu akan menghasilkan daftar menu atau dengan kata lain daftar hidangan menggunakan bahan yang dimasukkan sebelumnya pengguna. Dan ada juga rekomendasi terkait setelah membuka salah satu resep setelah pencarian. Tidak hanya itu, situs web ini juga dapat memberikan kebebasan untuk berinovasi, dengan cara semua pengguna dapat mengisi resep baru sesuai dengan inovasi dan kreasi itu sendiri. Kemudian resepnya akan dipublikasikan dan dibaca oleh publik. Kolektif Kecerdasan Kolektif dan Kemiringan Satu metode ini diimplementasikan dalam desain desain aplikasi ini.
PERANCANGAN SISTEM INFORMASI PARIWISATA KOTA SORONG MENGGUNAKAN WEB DAN LINE@ CHATBOT SEBAGAI MEDIA INFORMASI PARIWISATA
Authors: Kadaton Muhammad Sahid s , Soekarta Rendra
Published: August 2021

Abstrak Sorong merupakan salah satu kota yang di kelilingi objek wista alam dan berlokasi sangat strategis yaitu sebagai pintu gerbang Papua dan Papua Barat sehingga arus informasi juga bisnis sangat lancar disini. Tetapi kenyataannya masih banyak wisatawan domestik yang belum benar-benar mendapatkan informasi tentang produk lokal atau souvenir, dan industri pariwisata di Kota Sorong. Oleh karena itu wisatawan membutuhkan informasi yang bisa di dapat dengan mudah mengenai pariwisata kota sorong sehingga pariwisata yang ada di kota sorong dapat berkembang dengan baik dan berdampak pada pendapatan kota sorong di bidang pariwisata. maka Penulis ingin menerapkan sebuah chatbot yang dapat memberikan informasi seputar pariwisata kota sorong, dengan mengunakan layanan line chat bot sebagai media penyampaian informasi tempat wisata yang bisa interatif dan layanan line webhook maker sebagai layanan perancangan chat pada botnya dengan metode interaksi mengunakan keyword untuk memilih kategori dan lokasi tempat wisata.
Rancang Bangun Prototype Alat Pendeteksi Kebakaran Menggunakan Arduino Uno Dilengkapi Pemadam Dan Notifikasi Sms Gateway
Authors: Iskandar Alam Teguh Hidayat , Soekarta Rendra , Ramadhan Wahyu
Published: August 2021

Bencana kebakaran merupakan salah satu bencana yang dapat terjadi dimana saja dankapan saja. Kebakaran juga dapat disebabkan juga oleh kelalaina manusia, keadaan alam, danlain-lain. Kebakaran juga menjadi suatu permasalah yang dihadapi oleh masyarakat akibatketerlambatan petugas dalam penanganan mengakibatkan banyak kerugian yang bisa terjadi,korban jiwa ataupun materi. Para penghuni yang berada pada lokasi kebakaran yang terjadiharus mendapatkan informasi peringatan dini jika akan terjadinya kebaran mulai membesar.Maka dari itu tujuan penelitian ini adalah untuk merancang alat pendeteksi kebakaranmenggunakan arduino uno di lengkapi pemadam dengan notifikasi SMS gateway. Sistem iniakan bekerja dimana jika sensor flame dan sensor MQ-2 telah mendapat inputan Api atau asapmaka, secara otomatis modul GMS akan memberikan notifikasi berupa SMS ke HP.Sistem pendeteksi kebakaran ini juga dapat menggurangi dampak kebakaran yang akanmembesar dan dipadamkan oleh pompa air yang terpasang pada alat tersebut. Solusi alatpendeteksi kebakaran ini dapat meyelesaikan permasalahan yang dihadapi denganpenggiriman notifikasi SMS kepada petugas dan dapat ditanggulagi secara cepat oleh alattersebur.
Rancang Bangun Alat Pemberi Pakan Ikan Otomatis Pada Akuarium Berbasis Arduino Uno
Authors: Soekarta Rendra , Yapari Denny , Ackswan M.
Published: August 2021

Pemeliharaan ikan hias sangat digandrungi banyak orang, tak kala masuk waktu  pemberian pakan tiba kadang kita sering lupa dalam memberikan pakan, dan terkadang banyak juga ssang pemelihara punya ksesibukan tersendiri hingga lupa memberikan pakan pada ikan peliharaan. Dan banyak juga para penghobbyh ikan yang telah mempunyai fish deffer tapi sangat cepat sekali rusak dan kadang kerusakan tidak diketahui pemilik, dan ikan yang dipelihara dapat mati akibat tidak diberi pakan. Maka dari itu tujuan penelitian ini adalah untuk merancang alat pemberi pakan ikan otomatis. menggunakan arduino Uno, di lengkapi dengan notifikasi suara yang menggunakan dfplayer. Sistem ini akan bekerja dimana jika sensor ultrasonik, telah mengirimkan data bahwa pakan kurang dari 7cm maka sistem servo akan berputar ketika telah memasuki waktu pemberian pakan, dan jika pakan lebih dari 7cm sistem dfplayer, spiker dan lcd ini dapat membantu para penghobby ikan hias dalam pengawasan pakan ikan.
Evaluation of Cooling Performance of an Additive Manufactured Gravity Die Casting Mold with Conformal Cooling Channel
Authors: Park Yu Jin , Kim Eok Soo , Lee Jeong Hun , Choi Ji Hwan , Yoon Pil Hwan , Kang Ho Jeong , Kim Dong Hyun , Park Yong Ho , Ko Jong Wan
Published: August 2021

A cooling channel with an optimized design provides not only high throughput with gravity die casting, but also guarantees product quality. A conformal cooling channel (CC) whose structure follows the shapes or surfaces of the mold cavity has attracted great attention in the die casting industry, because it allows rapid and uniform cooling. However, implementing conformal cooling remains highly challenging, because the complicated geometries of CC are difficult to form using conventional fabrication methods such as drilling and milling. In recent years, advances in additive manufacturing (AM) technology have made it possible to fabricate products with complex and elaborate structures. In this paper, a gravity die casting mold with CC was designed and built using AM technology. The cooling channel performance was estimated and evaluated using an Al-Si-Cu alloy casting simulation and die casting experiments, respectively. The casting simulation results showed that the cooling performance of the CC was enhanced by ca. 10% compared with that of a conventional cooling channel. The experimental cooling performance of the CC improved by ca. 8% compared to that of a conventional cooling channel, and the increment in performance was consistent with the simulation results. In addition, microstructural evidence clarified that the effective cooling performance of CC could be attributed to the decrement (ca. 17%) of the secondary dendrite arm spacing (SDAS) of the Al-Si-Cu alloy. In this research, AM technology provides a novel way to fabricate functionally superior CC molds that are hardly producible with traditional methods.
Dynamic Solution Space Division-Based Methods for Calculating Reaction Deletion Strategies for Constraint-Based Metabolic Networks for Substance Production: DynCubeProd
Authors: Ma Yier , Tamura Takeyuki
Published: August 2021

Flux balance analysis (FBA) is a crucial method to analyze large-scale constraint-based metabolic networks and computing design strategies for strain production in metabolic engineering. However, as it is often non-straightforward to obtain such design strategies to produce valuable metabolites, many tools have been proposed based on FBA. Among them, GridProd, which divides the solution space into small squares by focusing on the cell growth rate and the target metabolite production rate to efficiently find the reaction deletion strategies, was extended to CubeProd, which divides the solution space into small cubes. However, as GridProd and CubeProd naively divide the solution space into equal sizes, even places where solutions are unlikely to exist are examined. To address this issue, we introduce dynamic solution space division methods based on CubeProd for faster computing by avoiding searching in places where the solutions do not exist. We applied the proposed method DynCubeProd to iJO1366, which is a genome-scale constraint-based model of Escherichia coli. Compared with CubeProd, DynCubeProd significantly accelerated the calculation of the reaction deletion strategy for each target metabolite production. In addition, under the anaerobic condition of iJO1366, DynCubeProd could obtain the reaction deletion strategies for almost 40% of the target metabolites that the elementary flux vector-based method, which is one of the most effective methods in existence, could not. The developed software is available on https://github.com/Ma-Yier/DynCubeProd.
Significance of High-Containment Biological Laboratories Performing Work During the COVID-19 Pandemic: Biosafety Level-3 and -4 Labs
Authors: Yeh Kenneth B. , Tabynov Kairat , Parekh Falgunee K. , Mombo Illich , Parker Kyle , Tabynov Kaissar , Bradrick Shelton S. , Tseng Ashley S. , Yang Ji-Rong , Gardiner Lolly , Olinger Gene , Setser Bradly
Published: August 2021

High containment biological laboratories (HCBL) are required for work on Risk Group 3 and 4 agents across the spectrum of basic, applied, and translational research. These laboratories include biosafety level (BSL)-3, BSL-4, animal BSL (ABSL)-3, BSL-3-Ag (agriculture livestock), and ABSL-4 laboratories. While SARS-CoV-2 is classified as a Risk Group 3 biological agent, routine diagnostic can be handled at BSL-2. Scenarios involving virus culture, potential exposure to aerosols, divergent high transmissible variants, and zoonosis from laboratory animals require higher BSL-3 measures. Establishing HCBLs especially those at BSL-4 is costly and needs continual investments of resources and funding to sustain labor, equipment, infrastructure, certifications, and operational needs. There are now over 50 BSL-4 laboratories and numerous BSL-3 laboratories worldwide. Besides technical and funding challenges, there are biosecurity and dual-use risks, and local community issues to contend with in order to sustain operations. Here, we describe case histories for distinct HCBLs: representative national centers for diagnostic and reference, nonprofit organizations. Case histories describe capabilities and assess activities during COVID-19 and include capacities, gaps, successes, and summary of lessons learned for future practice.
Identification of Uterine Microbiota in Infertile Women Receiving in vitro Fertilization With and Without Chronic Endometritis
Authors: Chen Weijun , Wei Kehong , He Xia , Wei Jing , Yang Lijuan , Li Lin , Chen Tingtao , Tan Buzhen
Published: August 2021

In vitro fertilization (IVF) is an important assisted reproductive technology in treating infertility, whose failure rate is still high. Studies suggested that uterine microbiota are related to women’s reproductive diseases and persisting intrauterine bacterial infectious conditions, such as chronic endometritis (CE), impairing the pregnant processes. However, the relationship between uterine microbiota and IVF outcomes is still an open question. In the present study, 94 patients diagnosed with infertility were enrolled and were divided into CE (E group, n = 25) and non-CE (NE group, n = 69) groups depending on the hysteroscopy and immunohistochemistry. Subsequently, E (Ep, n = 8 and Enp, n = 17) and NE (NEp, n = 41 and NEnp, n = 28) groups were divided into pregnancy and non-pregnancy groups depending on the IVF outcomes, respectively. The uterine fluids were collected and microbial profiles were examined through the V4 region of 16S rRNA gene high-throughput sequencing. The results demonstrated that patients with CE had significantly lower clinical pregnancy rate compared with the non-CE patients (32 vs. 58.42%, p = 0.0014). The relative abundances of Proteobacteria and Acidobacteria were higher in the non-CE group, whereas high abundances of Actinobacteria and Fusobacteria were observed in the CE group at the phylum level. At the genus level, high relative abundances of Gardnerella were observed in the CE group and non-pregnancy groups, which significantly referred to the negative IVF outcome. In conclusion, CE may be a key factor for the negative outcome after IVF, of which the uterine microbiota plays a pivotal role, and the microbial diversity in uterine may serve as a biomarker to forecast the success of IVF outcome.
The Incoherent Fluctuation of Folate Pools and Differential Regulation of Folate Enzymes Prioritize Nucleotide Supply in the Zebrafish Model Displaying Folate Deficiency-Induced Microphthalmia and Visual Defects
Authors: Hsiao Tsun-Hsien , Lee Gang-Hui , Chang Yi-Sheng , Chen Bing-Hung , Fu Tzu-Fun
Published: August 2021

Graphical Abstract

Folate deficiency lowered intracellular 10-CHO-THF and 5-CH3-THF levels, interfered nucleotide formation, impaired cell proliferation, induced apoptosis and impeded vitamin A supply, leading to microphthalmia and obstructed visual ability.
Hair Cell Generation in Cochlear Culture Models Mediated by Novel γ-Secretase Inhibitors
Authors: Erni Silvia T. , Gill John C. , Palaferri Carlotta , Fernandes Gabriella , Buri Michelle , Lazarides Katherine , Grandgirard Denis , Edge Albert S. B. , Leib Stephen L. , Roccio Marta
Published: August 2021

Sensorineural hearing loss is prevalent within society affecting the quality of life of 460 million worldwide. In the majority of cases, this is due to insult or degeneration of mechanosensory hair cells in the cochlea. In adult mammals, hair cell loss is irreversible as sensory cells are not replaced spontaneously. Genetic inhibition of Notch signaling had been shown to induce hair cell formation by transdifferentiation of supporting cells in young postnatal rodents and provided an impetus for targeting Notch pathway with small molecule inhibitors for hearing restoration. Here, the oto-regenerative potential of different γ-secretase inhibitors (GSIs) was evaluated in complementary assay models, including cell lines, organotypic cultures of the organ of Corti and cochlear organoids to characterize two novel GSIs (CPD3 and CPD8). GSI-treatment induced hair cell gene expression in all these models and was effective in increasing hair cell numbers, in particular outer hair cells, both in baseline conditions and in response to ototoxic damage. Hair cells were generated from transdifferentiation of supporting cells. Similar findings were obtained in cochlear organoid cultures, used for the first time to probe regeneration following sisomicin-induced damage. Finally, effective absorption of a novel GSI through the round window membrane and hair cell induction was attained in a whole cochlea culture model andin vivopharmacokinetic comparisons of transtympanic delivery of GSIs and different vehicle formulations were successfully conducted in guinea pigs. This preclinical evaluation of targeting Notch signaling with novel GSIs illustrates methods of characterization for hearing restoration molecules, enabling translation to more complex animal studies and clinical research.
Organoids and Their Use in Modeling Gut Epithelial Cell Lineage Differentiation and Barrier Properties During Intestinal Diseases
Authors: Gómez Dianne Pupo , Boudreau Francois
Published: August 2021

Maintenance of intestinal epithelium homeostasis is a complex process because of the multicellular and molecular composition of the gastrointestinal wall and the involvement of surrounding interactive signals. The complex nature of this intestinal barrier system poses challenges in the detailed mechanistic understanding of intestinal morphogenesis and the onset of several gut pathologies, including intestinal inflammatory disorders, food allergies, and cancer. For several years, the gut scientific community has explored different alternatives in research involving animals and in vitro models consisting of cultured monolayers derived from the immortalized or cancerous origin cell lines. The recent ability to recapitulate intestinal epithelial dynamics from mini-gut cultures has proven to be a promising step in the field of scientific research and biomedicine. The organoids can be grown as two- or three-dimensional structures, and are derived from adult or pluripotent stem cells that ultimately establish an intestinal epithelium that is composed of all differentiated cell types present in the normal epithelium. In this review, we summarize the different origins and recent use of organoids in modeling intestinal epithelial differentiation and barrier properties.
Assemblies of Polyacrylonitrile-Derived Photoactive Polymers as Blue and Green Light Photo-Cocatalysts for Cu-Catalyzed ATRP in Water and Organic Solvents
Authors: Sun Mingkang , Lorandi Francesca , Yuan Rui , Dadashi-Silab Sajjad , Kowalewski Tomasz , Matyjaszewski Krzysztof
Published: August 2021

Photoluminescent nanosized quasi-spherical polymeric assemblies prepared by the hydrothermal reaction of polyacrylonitrile (PAN), ht-PLPPAN, were demonstrated to have the ability to photo-induce atom transfer radical polymerization (ATRP) catalyzed by low, parts per million concentrations of CuII complex with tris(2-pyridylmethyl)amine (TPMA). Such photo induced ATRP reactions of acrylate and methacrylate monomers were performed in water or organic solvents, using ht-PLPPAN as the photo-cocatalyst under blue or green light irradiation. Mechanistic studies indicate that ht-PLPPAN helps to sustain the polymerization by facilitating the activation of alkyl bromide species by two modes: 1) green or blue light-driven photoreduction of the CuII catalyst to the activating CuI form, and 2) direct activation of dormant alkyl bromide species which occurs only under blue light. The photoreduction of the CuII complex by ht-PLPPAN was confirmed by linear sweep voltammetry performed under illumination. Analysis of the polymerization kinetics in aqueous media indicated even though CuI complexes comprised only 1–1.4% of all Cu species at equilibrium, they exhibited high activation rate constant and activated the alkyl bromide initiators five to six orders of magnitude faster than ht-PLPPAN.
The Chemokine CCL5 Inhibits the Replication of Influenza A Virus Through SAMHD1 Modulation
Authors: Silva Thauane , Temerozo Jairo R. , do Vale Gabriele , Ferreira André C. , Soares Vinícius Cardoso , Dias Suelen Silva Gomes , Sardella Gabriela , Bou-Habib Dumith Chequer , Siqueira Marilda , Souza Thiago Moreno L. , Miranda Milene
Published: August 2021

Influenza A virus (IAV) is the main etiological agent of acute respiratory tract infections. During IAV infection, interferon triggers the overexpression of restriction factors (RFs), the intracellular antiviral branch of the innate immune system. Conversely, severe influenza is associated with an unbalanced pro-inflammatory cytokine release. It is unclear whether other cytokines and chemokines released during IAV infection modulate RFs to control virus replication. Among the molecules enhanced in the infected respiratory tract, ligands of the CCR5 receptor play a key role, as they stimulate the migration of inflammatory cells to the alveoli. We investigated here whether ligands of the CCR5 receptor could enhance RFs to levels able to inhibit IAV replication. For this purpose, the human alveolar basal epithelial cell line (A549) was treated with endogenous (CCL3, CCL4 and CCL5) or exogenous (HIV-1 gp120) ligands prior to IAV infection. The three CC-chemokines tested reduced infectious titers between 30% to 45% upon 24 hours of infection. Eploying RT-PCR, a panel of RF mRNA levels from cells treated with CCR5 agonists was evaluated, which showed that the SAMHD1 expression was up-regulated four times over control upon exposure to CCL3, CCL4 and CCL5. We also found that IAV inhibition by CCL5 was dependent on PKC and that SAMHD1 protein levels were also increased after treatment with CCL5. In functional assays, we observed that the knockdown of SAMHD1 resulted in enhanced IAV replication in A549 cells and abolished both CCL5-mediated inhibition of IAV replication and CCL5-mediated cell death inhibition. Our data show that stimuli unrelated to interferon may trigger the upregulation of SAMHD1 and that this RF may directly interfere with IAV replication in alveolar epithelial cells.