bluuebunny/crossref_metadata_2025 huggingface dataset:
Qualidade pós-colheita de colmos de cana armazenados e seus reflexos na produção de cachaça
Authors: Oliveira Filho José Humberto de , Bortoletto Aline Marques , Alcarde André Ricardo
Published: July 2016

Resumo Esta pesquisa teve como objetivos avaliar as características tecnológicas e microbiológicas do caldo de cana colhida e armazenada, assim como seus reflexos na condução dos processos fermentativos e formação dos compostos secundários e contaminantes da cachaça. Depois da colheita da cana-de-açúcar, os colmos foram armazenados à temperatura ambiente (20-32 °C) por períodos de 0, 24, 48, 72 e 96 horas, sendo o mosto preparado logo após a extração do caldo. As fermentações foram conduzidas em processo de batelada, por cinco ciclos fermentativos, com destilação dos vinhos em alambique de cobre. Foram analisadas as características tecnológicas e microbiológicas do caldo e do processo fermentativo. O armazenamento dos colmos de cana causou significantes perdas na qualidade tecnológica do caldo, influenciando negativamente o processo fermentativo, com decréscimo na viabilidade de células e brotos de leveduras. A utilização de matéria-prima armazenada por períodos superiores a 48 horas contribuiu para a redução do pH e aumento da acidez total e açúcares redutores residuais dos vinhos, refletindo em menor produção de álcool. Os níveis de acidez volátil, acetato de etila, acetaldeído, cobre, metanol e carbamato de etila das cachaças ficaram dentro do estabelecido pela Legislação Brasileira, enquanto o conteúdo de álcoois superiores e furfural apresentaram-se elevados nos destilados de cana colhida e armazenada.
Quando um visitante floral é um polinizador?
Authors: Alves-dos-Santos Isabel , Silva Claudia Inês da , Pinheiro Mardiore , Kleinert Astrid de Matos Peixoto
Published: July 2016

Resumo Os visitantes florais são animais que buscam, nas flores, recursos para si ou para sua prole. Às vezes uma mesma planta pode receber um espectro grande de visitantes, como é o caso de espécies cujas flores oferecem muito néctar. Porém, nem todo visitante floral realiza a polinização. Para ser um polinizador efetivo é necessário cumprir alguns requisitos, como: contato com a antera e o estigma, frequência de visita suficiente, fidelidade floral e a realização de uma rota adequada de visitação. Em muitos trabalhos sobre a estrutura de comunidades e, principalmente, sobre a rede trófica (ex. animal-planta), os autores adotam o termo polinizador para todo e qualquer visitante floral, categorizando-os com o mesmo papel e função para a planta. Neste trabalho, relatamos casos de visitantes florais que são polinizadores legítimos e efetivos, dos quais a planta depende de seu serviço para produção de frutos e sementes, distinguindo-os daqueles visitantes que não efetuam a polinização, ou não se tem certeza que o façam. Assim, espera-se ilustrar as diferenças, dando à devida importância e o peso correto para os muitos visitantes. Utilizamos abelhas como modelo, mas a idéia pode ser extrapolada para qualquer outro grupo de polinizador.
Functional diversity of reproductive traits increases across succession in the Atlantic forest
Authors: Warring Bianca , Cardoso Fernanda Cristina Gil , Marques Marcia C.M. , Varassin Isabela Galarda
Published: July 2016

Abstract Niche and neutral processes shape community assembly with a possible shift of niche and neutral importance in communities undergoing temporal changes during succession. Functional diversity helps to discriminate assembly processes since trait distribution is dependent on those processes. We evaluated the changes in reproductive traits related to pollination and seed dispersal in a successional gradient in an Atlantic Forest area, Southern Brazil. We surveyed forests undergoing regeneration varying in age from 2 to 80 years after pasture abandonment. We expected an increase in functional diversity of reproductive traits and a greater role of limiting similarity across succession. Abiotic and mixed pollination systems, dioecious sexual system, biotic dispersed, many-seeded and small-seeded species decreased as the forest got older. Conversely, bee-pollinated, bell-shaped, small and androgynous flowers increased across forest succession as well biotic dispersed and large-seeded species. Functional richness and functional dispersion were higher in older forests. Changes in functional diversity were positively related to species richness, indicating that species enrichment in older forests added new sets of reproductive traits. These changes in trait distribution and functional diversity across succession in the Atlantic Forest suggest an increased role of biotic interactions and limiting similarity process structuring plant assemblages of second-growth tropical forests.
Sistema reprodutivo atípico de duas espécies de Rubiaceae: distilia com autoincompatibilidade parcial no morfo brevistilo?
Authors: Matias Raphael , Oliveira Alexandre Silva de , Furtado Marco Túlio , Sá Túlio , Rodrigues Ebenézer Barbosa , Oliveira Paulo Eugênio de , Consolaro Hélder
Published: July 2016

Resumo Espécies distílicas possuem populações com dois morfos florais. O morfo longistilo possui flores com pistilos longos e estames curtos e o morfo brevistilo, pistilos curtos e estames longos. Normalmente, os morfos apresentam um sistema heteromórfico de incompatibilidade e os morfos estão em uma razão de 1:1 (isopletia) nas populações. Diferentes variações podem ser encontradas em espécies distílicas, como a presença de autocompatibilidade, anisopletia e variações no nível de reciprocidade entre anteras e estigmas dos dois morfos. O objetivo desse trabalho foi investigar a ocorrência das características distílicas em duas espécies de Rubiaceae. Para isso, a razão dos morfos, a hercogamia recíproca, o sistema de incompatibilidade e os visitantes florais de Psychotria deflexa e Declieuxia fruticosa foram estudados. Ambas as espécies apresentaram os dois morfos em proporção semelhante, altos valores de reciprocidade entre a altura dos verticilos sexuais dos morfos florais e apenas insetos como visitantes florais. O morfo brevistilo das duas espécies apresentou autoincompatibilidade parcial. Dentro de Rubiaceae, mesmo em grupos filogenéticos distintos, o relaxamento ou quebra da distilia parece ocorrer em padrões similares e comumente tal variação é mais comum no morfo longistilo. Para as espécies estudadas, mesmo com a pseudocompatibilidade do morfo brevistilo, as populações apresentaram proporção igual dos morfos, indicando que fatores como a alta hercogamia recíproca e, possivelmente, o serviço de polinização podem promover a manutenção da isopletia característica da distilia.
Biologia reprodutiva de Campylocentrum micranthum (Orchidaceae, Angraecinae)
Authors: Cabral Paulo Roberto de Medeiros , Pansarin Emerson Ricardo
Published: July 2016

Resumo Campylocentrum micranthum é uma espécie amplamente distribuída pelo Brasil. A biologia reprodutiva de C. micranthum foi estudada no município de São Simão, no noroeste do estado de São Paulo. A vegetação da região é caracterizada por florestas semideciduais, matas palustres e cerrado. Na área de estudo C. micranthum floresce no verão. A população estudada é formada por mais de 35 indivíduos distribuídos em uma área de mata palustre. As plantas são epífitas, e as flores são ressupinadas e de coloração creme. Cada flor possui um nectário tubuloso na base que produz em média 0,053 µL de néctar. As atividades dos polinizadores se iniciam por volta das 07:30 e as visitas se estendem até 13:00 h. Os polinizadores de C. micranthum são abelhas dos gêneros Lophopedia e Ceratina. A espécie autocompatível e não autógama. No entanto, necessita de polinizadores para a transferência de pólen. Nos experimentos de polinização cruzada à formação de frutos foi de 3,17%, enquanto no tratamento de autopolinização manual foi obtido 16% de frutificação. Em condições naturais a taxa de frutificação foi de 36,4%.
Biologia reprodutiva e guilda de visitantes florais de Pseudobombax marginatum (Malvaceae)
Authors: Pequeno Izaac Damasceno , Almeida Natan Messias , Filho José Alves Siqueira
Published: July 2016

Resumo No Brasil, ocorrem 120 espécies do gênero Pseudobombax em diferentes formações vegetacionais. Espécies de Bombacoideae apresentam flores com antese noturna, visitadas por morcegos e esfingídeos. Objetivou-se investigar a biologia reprodutiva de P. marginatum, e conhecer relações entre planta e visitantes. O estudo foi desenvolvido em uma área de caatinga, no município de Afrânio, Pernambuco. Analisou-se a fenologia, biologia floral, comportamento dos visitantes e sistema reprodutivo. P. marginatum apresenta características de flores quiropterófilas, apesar de não receber visitas de morcegos, parecendo estar relacionado ao processo de fragmentação florestal ocorrente na área de estudo. A espécie floresceu entre maio e julho, recebendo visitas de esfingídeos, abelhas, vespas e aves. A produção de néctar foi elevada com baixa concentração de açúcares. Apenas na polinização cruzada obteve-se sucesso, devido à presença de mecanismos de incompatibilidade. P. marginatum apresenta-se em situação crítica quanto à sua regeneração, devido à carência de serviços de polinização eficientes, provavelmente ocasionados pela perturbação na área e ausência de quirópteros visitantes.
Análisis panbiogeográfico del género Galianthe subgénero Ebelia (Rubiaceae)
Authors: Florentin Javier E. , Arana Marcelo D. , Salas Roberto M.
Published: July 2016

Resumen Galianthe (Rubiaceae: Spermacoceae) es un género con los subgéneros Galianthe y Ebelia, éste último comprende 11 especies neotropicales, con centro de diversidad se encuentra en Brasil. El análisis panbiogeográfíco enfatiza la importancia de la dimensión espacial de la biodiversidad para generar una comprensión más adecuada de los patrones y procesos evolutivos y enfoca los eventos vicariantes como la mayor fuerza de la fragmentación de biotas. Son escasos los estudios biogeográficos de Galianthe, por lo que el objetivo del presente trabajo es establecer los patrones de distribución de Ebelia mediante un análisis de trazos. Las especies poseen distribución mesoamericana, andina y chaqueña, cuatro de estas últimas son exclusivas de Brasil. Siete especies conforman un único trazo generalizado denominado "Paraná", que conecta las provincias biogeográficas Cerrado y Chaco del dominio Chaqueño y Atlántica, Bosque Paraná y Bosque de Araucaria, pertenecientes al dominio Paraná, ambos dominios se hallan íntegramente dentro de la subregión Chaqueña, región Neotropical. Debido a que el origen de la tribu Spermacoceae se ha establecido durante el Eoceno, la aridificación de Sudamérica generada por los eventos vicariantes como la orogenia andina e introgresiones marinas, posiblemente han favorecido la radiación adaptativa del subgénero Ebelia dentro de la subregión Chaqueña.
What is the name of the babassu? A note on the confusing use of scientific names for this important palm tree
Authors: Cavallari Marcelo Mattos , Toledo Marcos Miranda
Published: July 2016

Abstract Various scientific names are usually found for the babassu in current literature: mainly Orbignya phalerata and Attalea speciosa, but also O. speciosa and O. martiana, among others. This multiplicity of names leads to great confusion in the scientific community causing mistakes to be successively propagated. This note aims to clarify the problem, reviewing the species nomenclature history since the first description by Martius, in 1826, and reporting the successive name changes which occurred. Consequences of the lumping of four closely related genera, of recent phylogenetic studies and of the last changes in palm classification on the species nomenclature are also briefly discussed. In addition, searching results on scientific names at the main Plant Name Indexes are presented here. As a conclusion, we recommend the adoption of Attalea speciosa Mart ex. Spreng as the most suitable name for the babassu and highlight that a broad taxonomic review of the Attalea group is necessary.
Avaliação para o início da alimentação oral de recém-nascidos pré-termo
Authors: Bolzan Geovana de Paula , Berwig Luana Cristina , Prade Leila Sauer , Cuti Lilian Kopp , Yamamoto Raquel Coube de Carvalho , Silva Ana Maria Toniolo da , Weinmann Angela Regina Maciel
Published: July 2016

RESUMO Objetivo avaliar a acurácia do Preterm Oral Feeding Readiness Scale - POFRAS para iniciar a alimentação oral de recém-nascidos pré-termo e verificar a concordância entre este instrumento e o instrumento de avaliação do Nível de Habilidade Oral. Métodos Foram avaliados 82 recém-nascidos pré-termo quanto à prontidão para o início da alimentação oral através do POFRAS e da avaliação do Nível de Habilidade Oral, durante a primeira alimentação oral. A acurácia do POFRAS foi estimada em relação à variável proficiência, por meio da Curva ROC (Receiver Operating Characteristic Curve). Para a análise da concordância entre os instrumentos, foi utilizado o coeficiente Kappa. Resultados A acurácia global do POFRAS foi de 71,29%. O ponto de corte 29 foi o que apresentou melhor equilíbrio entre sensibilidade e especificidade. O coeficiente Kappa mostrou fraca concordância entre os instrumentos na identificação dos RN aptos e inaptos a mamar por via oral (k=0.281). Conclusão a acurácia do POFRAS para o início da alimentação oral, estimada por meio da variável proficiência, foi semelhante à obtida com a técnica de translactação. Observou-se fraca concordância entre os instrumentos avaliados. Sugere-se, portanto, que estes instrumentos de avaliação sejam usados de forma complementar na prática clínica, uma vez que ambos apresentam aspectos importantes do comportamento alimentar do prematuro, que ao serem analisados conjuntamente permitirão orientar a conduta necessária para propiciar uma transição alimentar mais breve e eficaz para essa população.
Valores de nasalância para sílabas produzidas por falantes do Português Brasileiro
Authors: Marino Viviane Cristina de Castro , Cardoso Vanessa Moraes , Ramos Renata Giorgetto , Dutka Jeniffer de Cássia Rillo
Published: July 2016

RESUMO Objetivo o objetivo do estudo foi estabelecer valores de nasalância para sílabas produzidas por falantes do Português Brasileiro com diferentes idades e sexo. Métodos Participaram deste estudo 245 falantes do Português Brasileiro (121 do sexo masculino e 124 do sexo feminino), de ambos os sexos, divididos em quatro grupos de idade: 57 crianças, 61 adolescentes, 65 adultos jovens e 62 adultos. Valores de nasalância foram obtidos para um conjunto de 14 sílabas (dez orais e quatro nasais), utilizando o Nasômetro II 6400. Resultados Valores de nasalância mais altos foram encontrados para sílabas nasais quando comparadas às orais e para as sílabas constituídas pela vogal /i/ quando comparadas com a vogal /a/. Valores de nasalância mais altos foram obtidos para mulheres quando comparados aos dos homens e, particularmente, para os adultos. Conclusão Valores de nasalância das sílabas produzidas por falantes do Português Brasileiro demonstraram variação quanto às sílabas investigadas e também para variável sexo para falantes adultos.
The development of communication skills and the teacher’s performance in the nursing student’s perspective
Authors: Oliveira Karime Rodrigues Emilio de , Braga Eliana Mara
Published: July 2016

Objective To understand experiences in the development of communication skills and the teacher’s role in this teaching-learning process under the perspective of undergraduate nursing students by considering two types of curriculum organization. Method Descriptive study with a qualitative approach conducted in two public schools located in São Paulo state, Brazil. Data were collected by means of self-completed forms from 81 students in the second and fourth years of the undergraduate program. Results were analyzed in light of Content Analysis. Results Results showed that the development of such skills is related to: students’ individual characteristics, patients’ characteristics, those of the health-disease process, the health-care team’s profile and the theoretical knowledge acquired on communication in health-care provision and nursing. The teacher’s role was perceived as one that supports and encourages interactions with patients and health-care teams by teaching and providing orientation about interpersonal communication. Conclusions Students identify and value the importance of their teachers’ performance in the development and acquisition of communication skills. Additionally, students who experience active teaching-learning methodologies acknowledge the teacher as essential to provide opportunities for students to express their knowledge and thoughts.
Perception of nursing faculty on the care: Heidegger constructions
Authors: Sebold Luciara Fabiane , Kempfer Silvana Silveira , Girondi Juliana Balbinot Reis , Prado Marta Lenise
Published: July 2016

Objective To understand the perceptions of nursing teachers about care in the light of Heidegger’s framework. It was used as theoretical and methodological reference Hei- degger’s hermeneutics. Method To capture the meanings we used phenomenological interviews with 11 teachers. e data analysis is based on heideggerian hermeneutic. Results The way to be a nurse determines their way of life to the care that re ects the construction of experiences in the nursing worldliness. The existence of the nurse for nursing care is evidenced in care relations established between being careful and being caregiver, deciding the mode of being-there of the nurse who has before him and on the other the possibilities of care. Conclusions It is being in the world that the being-nurse is manifested in their subjectivity in care for sensitive, is the objectivity of scienti c care, and is in the interrelationship with being careful is that manifests the being of choices and existing decisions in his way of being.
The Illness Experience: Palliative Care Given the impossibility of Healing
Authors: Dantas Margarida Maria Florêncio , Amazonas Maria Cristina Lopes de Almeida
Published: July 2016

This paper presents a reflection about being terminally ill and the various ways that the subject has at its disposal to deal with this event. The objective is to understand the experience of palliation for patients undergoing no therapeutic possibilities of cure. The methodology of this study has the instruments to semi-structured interview, the participant observation and the field diary, and the Descriptive Analysis of Foucault’s inspiration how the narratives of the subjects were perceived. The Results of paper there was the possibility of looking at the experience of illness through the eyes of a subject position assumed by the very sick. As conclusion we have than when choosing palliative care, the terminally ill opts for a way to feel more comfortable and resists the impositions of the medical model of prolonging life.
Social Representations of Gynecologic Cancer Screening Assessment a Qualitative research on Ecuadorian women
Authors: Godoy Yolanda , Godoy Clara , Reyes Juan
Published: July 2016

The purpose of this work was to explore: knowledge, attitudes, and beliefs regarding gynecologic cancer screening on Ecuadorian women users of primary care facilities, to identify the social representations that users of health services make about these programs and their influence on the decision to undergo a screening. An exploratory and qualitative research design was held using focus groups and in-depth interviews for data collection. A narrative content analysis of the results was conducted. Women’s knowledge on gynecological cancer screening was confusing. Most frequent misconceptions related to the pap smear were: the belief that it could be useful for detecting pregnancy, ovarian cysts or infections. Most of the participants stated that the pap smear procedure is a traumatic and painful experience. Regarding to mammography women said it was used for sick woman and this procedure by itself may cause cancer.
The family experience of care in chronic situation
Authors: Bellato Roseney , Araújo Laura Filomena Santos de , Dolina Janderléia Valéria , Musquim Cleciene dos Anjos , Corrêa Geovana Hagata de Lima Souza Thaines
Published: July 2016

An essay that aims to reflect on the family experience of care in chronic situation, increasing the understanding of the family as the primary caregiver. It is based on comprehensive approach in studies conducted in three matrix searches from family care experiences. We have taken three axes to organize our reflections: a) conformation of family care in chronic situation, highlighting the multiple costs incurred to the family, which can exhaust the potential of care and establish or increase its vulnerability if it is not backed by networks support and sustenance; b) family rearrangements for the care, giving visibility to care cores in which many loved family members share the care, dynamic, plural and changeable way; c) self care modeling family care, pointing to the range of possibilities of the person taking care of diseased conditions supported by people close to them. We learn that the family takes care of itself in everyday life and in the illness experience, creating networks that can provide you support and sustenance. Thus, professionals in health practices should shape up in a longitudinal and very personal way, by reference to the family care, supporting him in what is his own.
Moral Distress in Family Health Strategy: experiences expressed by daily life
Authors: Cardoso Cecília Maria Lima , Pereira Maria Odete , Moreira Danielle de Araújo , Tibães Hanna Beatriz Bacelar , Ramos Flávia Regina Souza , Brito Maria José Menezes
Published: July 2016

Objective Understanding the Moral Suffering experiences expressed in the daily life of the Family Health Strategy. Method This is a case study with a qualitative approach, conducted between August and October 2014 in a municipality of Minas Gerais. The sample was represented by 28 professionals of family health teams. Data were collected through interviews with semi-structured questionnaires, observation, projective technique and submitted to thematic content analysis. Results The results indicate that routine issues of the health care system lead professionals to experience a challenging practice in dealing with daily situations that contradict their ethical precepts and can compromise the quality of work, becoming triggers of Moral Distress. Conclusions Social vulnerabilities such as domestic violence, poor socioeconomic conditions and organizational weaknesses of the health system were the main triggers of Moral Distress. Therefore, it is necessary to amplify this reflection by workers of the Family Health Strategy, aiming to encourage the minimization of suffering experiences, considering their ethical values.
Changing dietary habits among Akwen Xerente
Authors: Rocha Tatiana Evangelista da Silva , Silva Reijane Pinheiro da , Nascimento Maira Messias do
Published: July 2016

The research aimed to identify the current feed of Xerente people in Indian villages Porteira and Funil in the city of Tocantinia / TO. It was used a qualitative ethnographic approach. Conducted from September 2013 to August 2014. The data were presented in a diary, through participant observation of food practices with the guidance of a script. A process of dietary change that permeates the culture of this people is taking place. Factors such as the deficit in the planting gardens, the recent arrival of energy, and therefore the technology has allowed access to processed foods. But some families still maintain the farming of cassava, yam, and beans. The main animals that are hunted in the village are peccary, deer and armadillo. It was possible to point which foods are inserted in the Xerente feed and factors related to this situation.
Taking care of the newborn dying and their families: Nurses' experiences of neonatal intensive care
Authors: Almeida Fabiane de Amorim , Moraes Mariana Salim de , Cunha Mariana Lucas da Rocha
Published: July 2016

Objective To understand the experiences of nurses when caring for dying newborns and their families in the NICU; and redeem their perceptions about acting before the death and grieving process. Method A descriptive exploratory study with a qualitative approach, developed with nine nurses at the ICU of a hospital in São Paulo (SP), Brazil. Data was collected through semi-structured interviews and analyzed using the Collective Subject Discourse (CSD). Results Caring for newborns who are dying and their families is very difficult for nurses, due to the intense involvement. They seek strategies to deal with the situation and, before the newborn’s death, despite the suffering, express the feeling of accomplishment. Conclusions Facing death and grief triggers mechanisms that emerge life references, coming across painful issues. Learning to deal with these questions is a daily challenge for nurses of the NICU.
Tide assessment for the continental shelf situated in the southwestern Atlantic between the latitudes 19.8ºS and 21.2ºS
Authors: Chacaltana Julio Tomás Aquije , Jesus Leonardo Carvalho de , Barreto Fernando Túlio Camilo , Innocentini Valdir
Published: July 2016

Abstract The astronomical tide was numerically simulated by the MOHID model for the South-Central Region of Espírito Santo State (Brazil), between latitudes 19.8ºS and 21.2ºS. The grid nesting technique was used to transpose the boundary conditions from the larger domain to a more refined local domain (spatial resolution around 450 m), with tidal harmonics provided by the TPXO tide inverse model as boundary conditions for the larger domain. Results from the numerical model were compared with both the pre-existing tide harmonics and the harmonics calculated from measured data. From the maps of phase, amplitude, tidal ellipses and residual currents the main tidal kinematic characteristics in the study area were described. On the continental shelf, the results showed that the physiography and bathymetry of the region play an important role in the distribution of tidal co-phases, co-amplitudes, ellipses and residual currents. The MOHID model was able to reproduce satisfactorily the astronomical tide and thus can be used to study the behaviour of the tidal propagation in continental shelf areas.
Macromedusae of Southern Brazil: temporal variation, population structure and biochemical composition
Authors: Barba Fabiana Figueredo Molin De , Bazi Carla Camila , Pessatti Marcos Luiz , Resgalla Jr Charrid
Published: July 2016

Abstract The objectives of this study were to record the macromedusae species on the north coast of Santa Catarina, and to estimate their population dynamics and variations in their biochemical composition, based on the specimens obtained in the by-catch of artisanal shrimp fishing between January 2012 and October 2014. Cohort analysis was performed to evaluate the population dynamic of the most frequent species. Biochemical analyses of the umbrellas were conducted on their water, carbohydrate, protein, lipid, ash and metals contents. Six species of macromedusae were found: Chiropsalmus quadrumanus and Tamoya haplonema (Cubozoa); Olindias sambaquiensis and Rhacostoma atlanticum (Hydrozoa), and Chrysaora lactea and Lychnorhiza lucerna (Scyphozoa). The species with the highest occurrence, and typical of the region, were Olindias sambaquiensis and Rhacostoma atlanticum. These species showed high inter-year variability, the highest densities being observed between autumn and winter 2014, associated with a less severe winter and coastal water with higher salinity. The distribution of size classes of O. sambaquiensis suggests two well-defined cohorts in spring/summer and in autumn/winter. R. atlanticum showed continuous reproduction and inter-year variation but a high value for protein content related to the reproductive peak in autumn. However, the Cubozoa species showed higher organic matter and metal content than the other classes.
Spatial variability in the icthyoplankton structure of a subtropicalhypersaline lagoon
Authors: Rosa Judson da Cruz Lopes da , Alberto Mariana Dantas , Ribas Wanda Maria Monteiro , Neves Maria Helena Campos Baeta , Fernandes Lohengrin Dias de A.
Published: July 2016

Abstract The Lagoa de Araruama is a hypersaline ecosystem inhabited by distinct fish species, either permanently or during their reproductive season. Over recent years, some significant environmental changes have been observed in this ecosystem related to the sewage runoff, as salinity decrease (from 64 to 41 psu during the last 40 years) and nutrients increase. As both changes are thought to affect the ichthyoplankton assemblage, the present study aimed to evaluate all the potential relationships between salinity disruption and fish larvae distribution. Ichtyoplankton samples were collected monthly from January 2010 to March 2011 at eight sites in Araruama Lagoon by means of a WP2 plankton net equipped with a flowmeter. During this period, low egg densities were coincident with high salinity regions, suggesting that adults are avoiding to release their eggs under less favorable environmental conditions to the larvae. The uneven distribution of eggs and larvae inside the lagoon, as revealed by both spatial and temporal analyses lead us to suggest that changes in salinity have influenced the reproductive rhythms of those fish species that depend upon the Lagoa de Araruama.
Viability of gait speed test in hospitalized elderly patients
Authors: Martinez Bruno Prata , Batista Anne Karine Menezes Santos , Ramos Isis Resende , Dantas Júlio Cesar , Gomes Isabela Barboza , Forgiarini Júnior Luiz Alberto , Camelier Fernanda Rosa Warken , Camelier Aquiles Assunção
Published: July 2016

ABSTRACT Objective: The gait speed test (GST) is a physical test that can predict falls and aid in the diagnosis of sarcopenia in the elderly. However, to our knowledge, there have been no studies evaluating its reproducibility in hospitalized elderly patients. The objective of this study was to evaluate the safety and reproducibility of the six-meter GST (6GST) in hospitalized elderly patients. Methods: This repeated measures study involved hospitalized elderly patients (≥ 60 years of age) who underwent the 6GST by the fifth day of hospitalization, were able to walk without assistance, and presented no signs of dyspnea or pain that would prevent them from performing the test. The 6GST was performed three times in sequence, with a rest period between each test, in a level corridor. Gait speed was measured in meters/second. Reproducibility was assessed by comparing the means, intraclass correlation coefficients (ICCs) and Bland-Altman plots. Results: We evaluated 110 elderly patients in a total of 330 tests. All participants completed all of the tests. The comparisons between the speeds obtained during the three tests showed high ICCs and a low mean bias (Bland-Altman plots). The correlation and accuracy were greatest when the mean maximum speed was compared with that obtained in the third test (1.26 ± 0.44 m/s vs. 1.22 ± 0.44 m/s; ICC = 0.99; p = 0.001; mean bias = 0.04; and limits of agreement = −0.27 to 0.15). Conclusions: The 6GST was proven to be safe and to have good reproducibility in this sample of hospitalized elderly patients. The third measurement seems to correspond to the maximum speed, since the first two measurements underestimated the actual performance.
Analysis of the stability of housekeeping gene expression in the left cardiac ventricle of rats submitted to chronic intermittent hypoxia
Authors: Julian Guilherme Silva , Oliveira Renato Watanabe de , Tufik Sergio , Chagas Jair Ribeiro
Published: July 2016

ABSTRACT Obstructive sleep apnea (OSA) has been associated with oxidative stress and various cardiovascular consequences, such as increased cardiovascular disease risk. Quantitative real-time PCR is frequently employed to assess changes in gene expression in experimental models. In this study, we analyzed the effects of chronic intermittent hypoxia (an experimental model of OSA) on housekeeping gene expression in the left cardiac ventricle of rats. Analyses via four different approaches-use of the geNorm, BestKeeper, and NormFinder algorithms; and 2−ΔCt (threshold cycle) data analysis-produced similar results: all genes were found to be suitable for use, glyceraldehyde-3-phosphate dehydrogenase and 18S being classified as the most and the least stable, respectively. The use of more than one housekeeping gene is strongly advised.
Robotic pulmonary lobectomy for lung cancer treatment: program implementation and initial experience
Authors: Terra Ricardo Mingarini , Araujo Pedro Henrique Xavier Nabuco de , Lauricella Leticia Leone , Campos José Ribas Milanez de , Costa Herbert Felix , Pego-Fernandes Paulo Manuel
Published: July 2016

ABSTRACT Objective: To describe the implementation of a robotic thoracic surgery program at a public tertiary teaching hospital and to analyze its initial results. Methods: This was a planned interim analysis of a randomized clinical trial aimed at comparing video-assisted thoracoscopic surgery and robotic surgery in terms of the results obtained after pulmonary lobectomy. The robotic surgery program developed at the Instituto do Câncer do Estado de São Paulo, in the city of São Paulo, Brazil, is a multidisciplinary initiative involving various surgical specialties, as well as anesthesiology, nursing, and clinical engineering teams. In this analysis, we evaluated the patients included in the robotic lobectomy arm of the trial during its first three months (from April to June of 2015). Results: Ten patients were included in this analysis. There were eight women and two men. The mean age was 65.1 years. All of the patients presented with peripheral tumors. We performed right upper lobectomy in four patients, right lower lobectomy in four, and left upper lobectomy in two. Surgical time varied considerably (range, 135-435 min). Conversion to open surgery or video-assisted thoracoscopic surgery was not necessary in any of the cases. Intraoperative complications were not found. Only the first patient required postoperative transfer to the ICU. There were no deaths or readmissions within the first 30 days after discharge. The only postoperative complication was chest pain (grade 3), in two patients. Pathological examination revealed complete tumor resection in all cases. Conclusions: When there is integration and proper training of all of the teams involved, the implementation of a robotic thoracic surgery program is feasible and can reduce morbidity and mortality.
The value of antibody-coated bacteria in tracheal aspirates for the diagnosis of ventilator-associated pneumonia: a case-control study
Authors: Ranzani Otavio Tavares , Forte Daniel Neves , Forte Antonio Carlos , Mimica Igor , Forte Wilma Carvalho Neves
Published: July 2016

ABSTRACT Objective: Ventilator-associated pneumonia (VAP) is the leading type of hospital-acquired infection in ICU patients. The diagnosis of VAP is challenging, mostly due to limitations of the diagnostic methods available. The aim of this study was to determine whether antibody-coated bacteria (ACB) evaluation can improve the specificity of endotracheal aspirate (EA) culture in VAP diagnosis. Methods: We conducted a diagnostic case-control study, enrolling 45 patients undergoing mechanical ventilation. Samples of EA were obtained from patients with and without VAP (cases and controls, respectively), and we assessed the number of bacteria coated with FITC-conjugated monoclonal antibodies (IgA, IgM, or IgG) or an FITC-conjugated polyvalent antibody. Using immunofluorescence microscopy, we determined the proportion of ACB among a fixed number of 80 bacteria. Results: The median proportions of ACB were significantly higher among the cases (n = 22) than among the controls (n = 23)-IgA (60.6% vs. 22.5%), IgM (42.5% vs. 12.5%), IgG (50.6% vs. 17.5%), and polyvalent (75.6% vs. 33.8%)-p < 0.001 for all. The accuracy of the best cut-off points for VAP diagnosis regarding monoclonal and polyvalent ACBs was greater than 95.0% and 93.3%, respectively. Conclusions: The numbers of ACB in EA samples were higher among cases than among controls. Our findings indicate that evaluating ACB in EA is a promising tool to improve the specificity of VAP diagnosis. The technique could be cost-effective and therefore useful in low-resource settings, with the advantages of minimizing false-positive results and avoiding overtreatment.
Anatomic pulmonary resection by video-assisted thoracoscopy: the Brazilian experience (VATS Brazil study)
Authors: Terra Ricardo Mingarini , Kazantzis Thamara , Pinto-Filho Darcy Ribeiro , Camargo Spencer Marcantonio , Martins-Neto Francisco , Guimarães Anderson Nassar , Araújo Carlos Alberto , Losso Luis Carlos , Ghefter Mario Claudio , Lima Nuno Ferreira de , Gomes-Neto Antero , Brito-Filho Flávio , Haddad Rui , Saueressig Maurício Guidi , Lima Alexandre Marcelo Rodrigues , Siqueira Rafael Pontes de , Pinho Astunaldo Júnior de Macedo e , Vannucci Fernando
Published: July 2016

ABSTRACT Objective: The objective of this study was to describe the results of anatomic pulmonary resections performed by video-assisted thoracoscopy in Brazil. Methods: Thoracic surgeons (members of the Brazilian Society of Thoracic Surgery) were invited, via e-mail, to participate in the study. Eighteen surgeons participated in the project by providing us with retrospective databases containing information related to anatomic pulmonary resections performed by video-assisted thoracoscopy. Demographic, surgical, and postoperative data were collected with a standardized instrument, after which they were compiled and analyzed. Results: The surgeons provided data related to a collective total of 786 patients (mean number of resections per surgeon, 43.6). However, 137 patients were excluded because some data were missing. Therefore, the study sample comprised 649 patients. The mean age of the patients was 61.7 years. Of the 649 patients, 295 (45.5%) were male. The majority-521 (89.8%)-had undergone surgery for neoplasia, which was most often classified as stage IA. The median duration of pleural drainage was 3 days, and the median hospital stay was 4 days. Of the 649 procedures evaluated, 598 (91.2%) were lobectomies. Conversion to thoracotomy was necessary in 30 cases (4.6%). Postoperative complications occurred in 124 patients (19.1%), the most common complications being pneumonia, prolonged air leaks, and atelectasis. The 30-day mortality rate was 2.0%, advanced age and diabetes being found to be predictors of mortality. Conclusions: Our analysis of this representative sample of patients undergoing pulmonary resection by video-assisted thoracoscopy in Brazil showed that the procedure is practicable and safe, as well as being comparable to those performed in other countries.
Pre-treatment with dexamethasone attenuates experimental ventilator-induced lung injury
Authors: Reis Fernando Fonseca dos , Reboredo Maycon de Moura , Lucinda Leda Marília Fonseca , Bianchi Aydra Mendes Almeida , Rabelo Maria Aparecida Esteves , Fonseca Lídia Maria Carneiro da , Oliveira Júlio César Abreu de , Pinheiro Bruno Valle
Published: July 2016

ABSTRACT Objective: To evaluate the effects that administering dexamethasone before the induction of ventilator-induced lung injury (VILI) has on the temporal evolution of that injury. Methods: Wistar rats were allocated to one of three groups: pre-VILI administration of dexamethasone (dexamethasone group); pre-VILI administration of saline (control group); or ventilation only (sham group). The VILI was induced by ventilation at a high tidal volume. Animals in the dexamethasone and control groups were euthanized at 0, 4, 24, and 168 h after VILI induction. We analyzed arterial blood gases, lung edema, cell counts (total and differential) in the BAL fluid, and lung histology. Results: At 0, 4, and 24 h after VILI induction, acute lung injury (ALI) scores were higher in the control group than in the sham group (p < 0.05). Administration of dexamethasone prior to VILI induction decreased the severity of the lung injury. At 4 h and 24 h after induction, the ALI score in the dexamethasone group was not significantly different from that observed for the sham group and was lower than that observed for the control group (p < 0.05). Neutrophil counts in BAL fluid were increased in the control and dexamethasone groups, peaking at 4 h after VILI induction (p < 0.05). However, the neutrophil counts were lower in the dexamethasone group than in the control group at 4 h and 24 h after induction (p < 0.05). Pre-treatment with dexamethasone also prevented the post-induction oxygenation impairment seen in the control group. Conclusions: Administration of dexamethasone prior to VILI induction attenuates the effects of the injury in Wistar rats. The molecular mechanisms of such injury and the possible clinical role of corticosteroids in VILI have yet to be elucidated.
Impact of long-term treatment with inhaled corticosteroids and bronchodilators on lung function in a patient with post-infectious bronchiolitis obliterans
Authors: Calabrese Cecilia , Corcione Nadia , Rea Gaetano , Stefanelli Francesco , Meoli Ilernando , Vatrella Alessandro
Published: July 2016

ABSTRACT Post-infectious bronchiolitis obliterans (PIBO) is a small airways disease characterized by fixed airflow limitation. Therefore, inhaled bronchodilators and corticosteroids are not recommended as maintenance therapy options. The management of PIBO currently consists only of close monitoring of affected patients, aimed at the prevention and early treatment of pulmonary infections. In recent years, there has been an increase in the incidence of PIBO in the pediatric population. Patients with PIBO are characterized by a progressive decline in lung function, accompanied by a decrease in overall functional capacity. Here, we report the case of a relatively young man diagnosed with PIBO and followed for three years. After short- and long-term therapy with an inhaled corticosteroid/long-acting 2 agonist combination, together with an inhaled long-acting antimuscarinic, the patient showed relevant improvement of airway obstruction that had been irreversible at the time of the bronchodilator test. The lung function of the patient worsened when he interrupted the triple inhaled therapy. In addition, a 3-week pulmonary rehabilitation program markedly improved his physical performance.
INTERSPINOUS SPACER IN PERSISTENT DISCOGENIC PAIN: PERCUTANEOUS APPROACH OR OPEN TECHNIQUE
Authors: Cruz Ricardez José Antonio , Romero Arroyo Jesús Alfonso Cao
Published: July 2016

ABSTRACT Objective: To compare the postoperative clinical course of placement of interspinous spacer with open technique (ISO) with percutaneous interspinous spacer (PIS). Methods: Quasi-experimental, longitudinal study of 42 patients with discogenic pain uncontrolled with analgesics, aged 35-55 years old, 21 women, and 21 men. Clinical history, location of pain, VAS scale before and after surgery, Oswestry Disability Index and Macnab modified scale at 6 months were used. Results: When performing quantitative analysis statistical significance (p = 0.0478, 0.0466, 0.0399) was demonstrated with Student's t test between the results according to VAS scale; in the qualitative analysis with the Oswestry index and Macnab modified scale it was demonstrated the hypothesis that the results is dependent of the surgical technique. Conclusions: According to the results, we can conclude that there is a statistically significant difference depending on the surgical technique used with respect to the rate of disability and functionality in daily life as well as in the improvement of pain symptoms.
GLOBAL BALANCE IN ADULT SPINAL DEFORMITIES. A STUDY OF 60 CASES
Authors: Galicia Luna Victor Misael , Guzman Carranza Juan Enrique , Gonzalez Moga Amado , Huerta Hernandez Gabriel , Isais Gomez Abraham , Anaya Contreras Victor Hugo , Alvarez Vazquez Leonardo
Published: July 2016

ABSTRACT Objectives: Determine the most prevalent type of curve in our population, to quantify the radiographic parameters such as PT, IP, SS and compare the physical function according to ODI and SRS-22r. Methods: Retrospective, observational, longitudinal, single-center study, carried out from January 2010 to May 2015 at the Centro Médico ISSEMYM Ecatepec, Spine Surgery Service. Results: A total of 60 patients were obtained, 60% female, with curvatures according to SRS-Schwab, type T (28%), TL (46.6%), D (15%), N (10%), with a mean preoperative VAS of 7 for all curves and post-surgical 2 after 6 months. The SRS-22r preoperative test was 2.1 and postoperatively was 3.75, with p<0.001. Conclusions: Deformities of the adult spine are a growing disease in our country. The surgical management of deformities requires proper clinical and radiographic planning. Patients undergoing surgical treatment in our study showed curvature type TL and demonstrated significant improvement in ODI and SRS-22r.
ANALYSIS OF 111 PATIENTS WITH CERVICAL SPINE FRACTURES IN A TERTIARY HOSPITAL
Authors: Vilchis Sámano Hugo , Villegas Esquivel Edgar , Muñiz Luna Luis
Published: July 2016

ABSTRACT Objective: To describe the epidemiology of 111 patients to determine the age group affected, the level of fracture, fracture classification, and type of treatment. Methods: Descriptive study of 111 cases of cervical spine fracture treated at the Hospital of Traumatology and Orthopedics Lomas Verdes - IMSS from March 2009 to October 2014. Results: The condition appeared in 88% of men. The age range was 16-81 years, with an average of 40 years. The most common fracture according to the AO classification was type A (57%). The segments most affected were C5-C6, C6-C7, C6 and C4-C5 (24.32%, 17.1%, 9.9% and 8.1%). Neurological involvement occurred in 46.8%. The most common mechanism of injury was high energy. Surgical treatment was performed in 78.4% of patients, being one-level corpectomy with placement of mesh and plate, and posterior fixation with facet screws the most appropriate procedures (31.5% and 27.9%, respectively). Conclusions: Males were the most affected, the highest incidence was in C5-C6 and C6-C7 levels and the surgical procedure was the most used.
RESULTS OF TREATMENT OF CERVICAL DISCOPATHY WITH PEEK INTERBODY CAGES AT THREE LEVELS WITHOUT PLATE FIXATION
Authors: González Moga Amado , Guzmán Carranza Enrique , Álvarez Vázquez Leonardo , Huerta Hernández Gabriel , Galicia Luna Víctor , Anaya Contreras Víctor Hugo , Isais Gómez Abraham
Published: July 2016

ABSTRACT Objective: To present the results of treatment of patients with cervical discopathy by anterior cervical approach, discectomy and placement of a PEEK interbody cage without anterior plate fixation. Methods: Retrospective, cross-sectional study from March 2013 to March 2015. Sixteen patients with radiculopathy or clinical signs of myelopathy were included; all patients underwent cervical surgery through anterior approach, discectomy, and placement of PEEK cages on three levels. Decompression levels were determined according to the correlation between preoperative radiological and clinical findings. Results: Sixteen patients predominantly male were included, with mean age of 50 years at the onset of the condition. Ten patients had involvement of C4-C5, C5-C6, C6-C7 levels, and six patients C3-C4, C4-C5 and C5-C6. Fourteen patients had cervicobrachialgia and two myelopathy. The preoperative visual analog scale average was 8/10 and the average postoperative value at 6 months was 3/10. At 6 months, there was no radiological evidence of recurrence. One patient had non-fatal complications. Conclusions: The treatment of cervical discopathy by anterior approach with interbody fusion with PEEK cage on three levels, with no plate fixation seemed to be safe and effective with better long-term results in terms of pain and myelopathy. The clinical results compare favorably with other similar series and, most importantly, the complications associated with anterior fixation plate are avoided.