bluuebunny/crossref_metadata_2025 huggingface dataset:
Trajectories of Adjustment Disorder and Well-Being in Austria and Croatia during 20 Months of the COVID-19 Pandemic
Authors: Zrnić Novaković Irina , Streicher Alina , Ajduković Dean , Ajduković Marina , Kiralj Lacković Jana , Lotzin Annett , Lueger-Schuster Brigitte
Published: September 2023

The present study aimed to investigate the trajectories of adjustment disorder (AD) symptoms and well-being over 20 months of the COVID-19 pandemic in Austria and Croatia. Further objectives of this study were to examine whether sociodemographic characteristics and the symptoms of anxiety and depression could predict these trajectories. As part of the pan-European ESTSS ADJUST study, N = 1144 individuals were recruited using convenience sampling and assessed four times between June 2020 and January 2022 through an online survey. Latent growth curve modelling was applied to estimate the trajectories of AD symptoms and well-being. Over time, the prevalence of probable AD varied between 9.8% and 15.1%. The symptoms of AD tended to increase, whereas well-being tended to decrease. According to the majority of the models tested, women, participants from Austria and those with lower income had higher initial AD symptoms, whereas older participants and those from Croatia had higher initial well-being. In all models and at all timepoints, anxiety and depression significantly predicted AD and well-being scores. Overall, our study points to several predictors of AD and well-being and indicates high variability in people’s reactions to the pandemic. Psychosocial support for the general population is needed during pandemics and similar crises, with a special focus on vulnerable groups.
Investigating the Effects of a New Peptide, Derived from the Enterolobium contortisiliquum Proteinase Inhibitor (EcTI), on Inflammation, Remodeling, and Oxidative Stress in an Experimental Mouse Model of Asthma–Chronic Obstructive Pulmonary Disease Overlap (ACO)
Authors: Barbosa Jéssica Anastácia Silva , da Silva Luana Laura Sales , João Juliana Morelli Lopes Gonçalves , de Campos Elaine Cristina , Fukuzaki Silvia , Camargo Leandro do Nascimento , dos Santos Tabata Maruyama , dos Santos Henrique Tibucheski , Bezerra Suellen Karoline Moreira , Saraiva-Romanholo Beatriz Mangueira , Lopes Fernanda Degobbi Tenório Quirino dos Santos , Bonturi Camila Ramalho , Oliva Maria Luiza Vilela , Leick Edna Aparecida , Righetti Renato Fraga , Tibério Iolanda de Fátima Lopes Calvo
Published: September 2023

The synthesized peptide derived from Enterolobium contortisiliquum (pep3-EcTI) has been associated with potent anti-inflammatory and antioxidant effects, and it may be a potential new treatment for asthma–COPD overlap—ACO). Purpose: To investigate the primary sequence effects of pep3-EcTI in an experimental ACO. BALB/c mice were divided into eight groups: SAL (saline), OVA (ovalbumin), ELA (elastase), ACO (ovalbumin + elastase), ACO-pep3-EcTI (treated with inhibitor), ACO-DX (treated with dexamethasone), ACO-DX-pep3-EcTI (treated with dexamethasone and inhibitor), and SAL-pep3-EcTI (saline group treated with inhibitor). We evaluated the hyperresponsiveness to methacholine, exhaled nitric oxide, bronchoalveolar lavage fluid (BALF), mean linear intercept (Lm), inflammatory markers, tumor necrosis factor (TNF-α), interferon (IFN)), matrix metalloproteinases (MMPs), growth factor (TGF-β), collagen fibers, the oxidative stress marker inducible nitric oxide synthase (iNOS), transcription factors, and the signaling pathway NF-κB in the airways (AW) and alveolar septa (AS). Statistical analysis was conducted using one-way ANOVA and t-tests, significant when p < 0.05. ACO caused alterations in the airways and alveolar septa. Compared with SAL, ACO-pep3-EcTI reversed the changes in the percentage of resistance of the respiratory system (%Rrs), the elastance of the respiratory system (%Ers), tissue resistance (%Gtis), tissue elastance (%Htis), airway resistance (%Raw), Lm, exhaled nitric oxide (ENO), lymphocytes, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, TNF-α, INF-γ, MMP-12, transforming growth factor (TGF)-β, collagen fibers, and iNOS. ACO-DX reversed the changes in %Rrs, %Ers, %Gtis, %Htis, %Raw, total cells, eosinophils, neutrophils, lymphocytes, macrophages, IL-1β, IL-6, IL-10, IL-13, IL-17, TNF-α, INF-γ, MMP-12, TGF-β, collagen fibers, and iNOS. ACO-DX-pep3-EcTI reversed the changes, as was also observed for the pep3-EcTI and the ACO-DX-pep3-EcTI. Significance: The pep3-EcTI was revealed to be a promising strategy for the treatment of ACO, asthma, and COPD.
Perspectives on Scaffold Designs with Roles in Liver Cell Asymmetry and Medical and Industrial Applications by Using a New Type of Specialized 3D Bioprinter
Authors: Harbuz Iuliana , Banciu Daniel Dumitru , David Rodica , Cercel Cristina , Cotîrță Octavian , Ciurea Bogdan Marius , Radu Sorin Mihai , Dinescu Stela , Jinga Sorin Ion , Banciu Adela
Published: September 2023

Cellular asymmetry is an important element of efficiency in the compartmentalization of intracellular chemical reactions that ensure efficient tissue function. Improving the current 3D printing methods by using cellular asymmetry is essential in producing complex tissues and organs such as the liver. The use of cell spots containing at least two cells and basement membrane-like bio support materials allows cells to be tethered at two points on the basement membrane and with another cell in order to maintain cell asymmetry. Our model is a new type of 3D bioprinter that uses oriented multicellular complexes with cellular asymmetry. This novel approach is necessary to replace the sequential and slow processes of organogenesis with rapid methods of growth and 3D organ printing. The use of the extracellular matrix in the process of bioprinting with cells allows one to preserve the cellular asymmetry in the 3D printing process and thus preserve the compartmentalization of biological processes and metabolic efficiency.
The Resistance of Maize to Ustilago maydis Infection Is Correlated with the Degree of Methyl Esterification of Pectin in the Cell Wall
Authors: Huang Yingni , Li Yang , Zou Kunkun , Wang Yang , Ma Yuting , Meng Dexuan , Luo Haishan , Qu Jianzhou , Li Fengcheng , Xuan Yuanhu , Du Wanli
Published: September 2023

Common smut caused by Ustilago maydis is one of the dominant fungal diseases in plants. The resistance mechanism to U. maydis infection involving alterations in the cell wall is poorly studied. In this study, the resistant single segment substitution line (SSSL) R445 and its susceptible recurrent parent line Ye478 of maize were infected with U. maydis, and the changes in cell wall components and structure were studied at 0, 2, 4, 8, and 12 days postinfection. In R445 and Ye478, the contents of cellulose, hemicellulose, pectin, and lignin increased by varying degrees, and pectin methylesterase (PME) activity increased. The changes in hemicellulose and pectin in the cell wall after U. maydis infection were analyzed via immunolabeling using monoclonal antibodies against hemicellulsic xylans and high/low-methylated pectin. U. maydis infection altered methyl esterification of pectin, and the degree of methyl esterification was correlated with the resistance of maize to U. maydis. Furthermore, the relationship between methyl esterification of pectin and host resistance was validated using 15 maize inbred lines with different resistance levels. The results revealed that cell wall components, particularly pectin, were important factors affecting the colonization and propagation of U. maydis in maize, and methyl esterification of pectin played a role in the resistance of maize to U. maydis infection.
Technical Implications of the Chicken Embryo Chorioallantoic Membrane Assay to Elucidate Neuroblastoma Biology
Authors: Patiño-Morales Carlos César , Jaime-Cruz Ricardo , Ramírez-Fuentes Tania Cristina , Villavicencio-Guzmán Laura , Salazar-García Marcela
Published: September 2023

The chorioallantoic membrane (CAM) can be used as a valuable research tool to examine tumors. The CAM can be used to investigate processes such as migration, invasion, and angiogenesis and to assess novel antitumor drugs. The CAM can be used to establish tumors in a straightforward, rapid, and cost-effective manner via xenotransplantation of cells or tumor tissues with reproducible results; furthermore, the use of the CAM adheres to the three “R” principle, i.e., replace, reduce, and refine. To achieve successful tumor establishment and survival, several technical aspects should be taken into consideration. The complexity and heterogeneity of diseases including neuroblastoma and cancers in general and their impact on human health highlight the importance of preclinical models that help us describe tumor-specific biological processes. These models will not only help in understanding tumor biology, but also allow clinicians to explore therapeutic alternatives that will improve current treatment strategies. In this review, we summarize the technical characteristics as well as the main findings regarding the use of this model to study neuroblastoma for angiogenesis, metastasis, drug sensitivity, and drug resistance.
Multispectral Raman Differentiation of Malignant Skin Neoplasms In Vitro: Search for Specific Biomarkers and Optimal Wavelengths
Authors: Rimskaya Elena , Shelygina Svetlana , Timurzieva Alina , Saraeva Irina , Perevedentseva Elena , Melnik Nikolay , Kudrin Konstantin , Reshetov Dmitry , Kudryashov Sergey
Published: September 2023

Confocal scanning Raman and photoluminescence (PL) microspectroscopy is a structure-sensitive optical method that allows the non-invasive analysis of biomarkers in the skin tissue. We used it to perform in vitro diagnostics of different malignant skin neoplasms at several excitation wavelengths (532, 785 and 1064 nm). Distinct spectral differences were noticed in the Raman spectra of basal cell carcinoma (BCC) and squamous cell carcinoma (SCC), compared with healthy skin. Our analysis of Raman/PL spectra at the different excitation wavelengths enabled us to propose two novel wavelength-independent spectral criteria (intensity ratios for 1302 cm−1 and 1445 cm−1 bands, 1745 cm−1 and 1445 cm−1 bands), related to the different vibrational “fingerprints” of cell membrane lipids as biomarkers, which was confirmed by the multivariate curve resolution (MCR) technique. These criteria allowed us to differentiate healthy skin from BCC and SCC with sensitivity and specificity higher than 95%, demonstrating high clinical importance in the differential diagnostics of skin tumors.
Color Stability, Gloss Retention, and Surface Roughness of 3D-Printed versus Indirect Prefabricated Veneers
Authors: Daghrery Arwa
Published: September 2023

The long-term color stability and surface properties of anterior laminate veneers are among the crucial factors affecting the clinical longevity of aesthetic restorations. Novel 3D-printed materials are being introduced as definitive restorative treatment. In light of the existing variety of indirect yet minimally invasive composite resin veneers, research on their surface properties is warranted. This in vitro study evaluated the effect of artificial aging by immersion in different staining solutions on the color changes, gloss, and surface roughness (Ra) of 3D-printed veneers compared to the prefabricated resin composite veneer systems (PRCVs) manufactured by Componeer and Edelweiss. Moreover, this study compared the effects of two methods for stain removal: repolishing with Sof-Lex disks and in-office bleaching with 40% hydrogen peroxide. The veneers (n = 24) were randomly divided according to the immersion solutions used, i.e., tea and coffee. Colorimetric measurements, surface roughness, and surface gloss were determined before and after staining and surface treatment with either in-office bleaching or surface polishing. The data were statistically analyzed using two-way ANOVA followed by the Tukey’s post hoc test (α = 0.05). Artificial aging with immersion in staining solutions led to significant color changes, increased surface roughness, and gloss reduction in all materials (p  <  0.05). The 3D-printed veneers showed higher ΔE values (coffee = 10.112 ± 0.141) and (tea = 10.689 ± 0.771) compared to baseline after 7 days of aging. The 3D-printed veneers had a statistically significant surface roughness Ra (0.574 µm ± 0.073). The gloss was >70% in all groups at baseline; these values dropped in all groups after 7 days of artificial aging. After the stain-removing procedures, the ΔE values decreased in all tested veneers. That being said, they failed to return to the baseline values, and both stain-removing methods were found to have an adverse effect on surface roughness and gloss retention in all tested veneers.
IL10 Gene and Neurodegenerative Sclerosis: A Systematic Review and Meta-Analysis
Authors: de Almeida Ismael Duarte Machado , de Paiva Pedro Luiz , Santos Kamilla de Faria , Reis Angela Adamski da Silva , Santos Rodrigo da Silva
Published: September 2023

Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS) are incurable degenerative scleroses with unclear etiology. Neuroinflammation is an important factor in the neurodegeneration characteristic of these diseases. Additionally, Interleukin 10 (IL10) can inhibit the synthesis of inflammatory cytokines and plays a protective role against neurodegeneration associated with neuroinflammation. Thus, we developed a systematic review and meta-analysis in order to clarify the relationship between polymorphisms in the IL10 gene and MS and/or ALS. We searched for observational studies in four international databases without time restrictions. Seventeen studies were added to the systematic review and six polymorphisms were observed: IL10-592 (rs1800872; C>A), IL10-819 (rs1800871; C>T), IL10-1082 (rs1800896; A>G), IL10-2763 (rs6693899; A>C), IL10-2849 (rs6703630; A>G) and IL10-3575 (rs1800890; A>T). In the meta-analysis, we used odds ratio (OR) and 95% confidence interval (CI) to evaluate the association of IL10-1082, IL10-819 and IL10-592 polymorphisms and MS. We found a positive association of MS with the IL10-1082 SNP in genotypic comparison (AG+GG vs. AA) (OR = 1.23; 95% CI = 1.01–1.51; p = 0.04). Our search did not find any article relating polymorphisms in the IL10 gene with ALS. Therefore, our analysis indicates a possible association of IL10 gene SNPs in the development and progression of MS.
Polypeptides Targeting Paracoccidioides brasiliensis Drk1
Authors: Marcos Caroline Maria , de Oliveira Haroldo Cesar , Assato Patricia Akemi , de Oliveira Lariane Teodoro , Fregonezi Nathália , dos Santos Kelvin Sousa , Costa-Orlandi Caroline Barcelos , Fusco-Almeida Ana Marisa , Mendes-Giannini Maria José Soares
Published: September 2023

Considering the toxicity of conventional therapeutic approaches and the importance of precise mechanistic targets, it is important to explore signaling pathways implicated in fungal pathobiology. Moreover, treatment of paracoccidioidomycosis, a systemic mycosis caused by a dimorphic fungus, requires prolonged therapeutic regimens. Among the numerous factors underpinning the establishment of Paracoccidioides spp. infection, the capacity to transition from the mycelial to the yeast form is of pivotal importance. The Drk1 protein of Paracoccidioides brasiliensis likely plays a decisive role in this morphological shift and subsequent virulence. We identified peptides with affinity for the PbDrk1 protein using the phage-display method and assessed the effects of these peptides on P. brasiliensis. The peptides were found to inhibit the phase transition of P. brasiliensis. Furthermore, a substantial proportion of these peptides prevented adhesion to pneumocytes. Although these peptides may not possess inherent antifungal properties, they can augment the effects of certain antifungal agents. Notably, the cell wall architecture of P. brasiliensis appears to be modulated by peptide intervention, resulting in a reduced abundance of glycosylated proteins and lipids. These peptides were also evaluated for their efficacy in a Galleria mellonella model and shown to contribute to enhanced larval survival rates. The role of PbDrk1, which is notably absent in mammals, should be further investigated to improve the understanding of its functional role in P. brasiliensis, which may be helpful for designing novel therapeutic modalities.
On the Direction of Causality between Business and Financial Cycles
Authors: Tsiakas Ilias , Zhang Haibin
Published: September 2023

This paper investigates whether business cycles cause financial cycles or vice versa. We also assess whether the US plays a leading role in causing the domestic business and financial cycles of other countries. The literature has established that business and financial cycles are linked through several channels such as credit constraints, the real effects of financial information and the reversal of overoptimistic expectations. Our analysis evaluates the direction of Granger causality using a novel approach based on the mixed-frequency vector autoregression model for the G7 countries. Our approach exploits the fact that real economic activity measured by industrial production is observed at a higher frequency than aggregate credit. We find strong evidence of bidirectional causality between the business and financial cycles, especially in recessions. Furthermore, the US is a global leader since the US business cycle significantly affects other countries’ business cycles, especially in terms of expansions.
SQMetrics: An Educational Software Quality Assessment Tool for Java
Authors: Sofronas Dimitrios , Margounakis Dimitrios , Rigou Maria , Tambouris Efthimios , Pachidis Theodore
Published: September 2023

Over the years, various software quality measurement models have been proposed and used in academia and the software industry to assess the quality of produced code and to obtain guidelines for its improvement. In this article, we describe the design and functionality of SQMetrics, a tool for calculating object-oriented quality metrics for projects written in Java. SQMetrics provides the convenience of measuring small code, mainly covering academic or research needs. In this context, the application can be used by students of software engineering courses to make measurements and comparisons in their projects and gradually increase their quality by improving the calculated metrics. Teachers, on the other hand, can use SQMetrics to evaluate students’ Java projects and grade them in proportion to their quality. The contribution of the proposed tool is three-fold, as it has been: (a) tested for its completeness and functionality by comparing it with widely known similar tools, (b) evaluated for its usability and value as a learning aid by students, and (c) statistically tested for its value as a teachers’ aid assisting in the evaluation of student projects. Our findings verify SQMetrics’ effectiveness in helping software engineering students learn critical concepts and improve the quality of their code, as well as in helping teachers assess the quality of students’ Java projects and make more informed grading decisions.
Development of Mechanical Properties of Stainless Steel 316LN-IG after Cryo-Plastic Deformation
Authors: Fedoriková Alica , Petroušek Patrik , Kvačkaj Tibor , Kočiško Róbert , Zemko Michal
Published: September 2023

The article deals with increasing the mechanical properties of stainless steel 316 Ln-IG, which is intended for work in cryogenic temperatures (liquid nitrogen and liquid helium), such as conductor conduits for the ITER magnet system. The strength and plastic properties were increased by a combination of cold and cryo-rolling and heat treatment. The mechanical properties of rolled material were investigated at 293 K, 77 K, and 4.2 K. The work-hardening rate of the steel increased continuously with a lowering of the temperature. The maximum yield strength and ultimate tensile strength were achieved by the cryo-rolling process with a total thickness deformation of 50%. The material properties tested at ambient temperature were 0.2YS = 1050 MPa, UTS = 1200 MPa, and at 4.2 K, the values were 0.2YS = 1804 MPa and UTS = 2081 MPa. Two types of long-term heat treatment were applied after experimental rolling (823 K and 1093 K for 10 h). The highest precipitation hardening of steel was achieved at a temperature of 823 K after 50% deformation. The resulting grain size decreased from the initial 216 μm (before the rolling process) to 70 μm after ambient rolling and 72 μm after cryo-rolling.
A Free-Standing Polymer Polypyrrole/Cellulose Composite Film via Spatial-Confined Interfacial Electrodeposition for Flexible Supercapacitors
Authors: Wang Sijie , Chen Wen , Huang Xinyue , Chen Xuezheng , Li De , Yu Feng , Chen Yong
Published: September 2023

As a kind of energy storage device, a flexible supercapacitor has the characteristics of high capacity, fast charge/discharge rate, good stability, portability and softness. Conductive polymer polypyrrole (PPy) can be used as an electrode material for supercapacitors due to its environmental friendliness, simple synthesis process, good conductivity and potential for large-scale production. However, pristine PPy inevitably suffers from structural rupture due to repeated doping/de-doping during charge and discharge processes, which in turn impairs its cycle stability. In general, compounding with flexible substrates like soft carbon materials, cellulose or nylon fabric, is a good strategy to weaken the inner stress and restrain the structure pulverization of PPy. Herein, cellulose is utilized as a soft substrate to compound with PPy based on the electrochemical oxidation of polypyrrole. The interfacial electrodeposition method can successfully obtain a smooth, uniform and flexible PPy/cellulose composite film, which shows good conductivity. The assembled symmetric supercapacitor with PPy/cellulose film has an optimized specific capacitance of 256.1 mF cm−2, even after 10,000 cycles at a current density of 1 mA cm−2. Furthermore, there is no significant capacitance loss even after 180° bending of the device. This work provides a new means to prepare flexible, low-cost, environmentally friendly and high-performance electrode materials for energy conversion and storage systems.
A SIRT6 Inhibitor, Marine-Derived Pyrrole-Pyridinimidazole Derivative 8a, Suppresses Angiogenesis
Authors: Song Nannan , Tang Yanfei , Wang Yangui , Guan Xian , Yu Wengong , Jiang Tao , Lu Ling , Gu Yuchao
Published: September 2023

Angiogenesis refers to the process of growing new blood vessels from pre-existing capillaries or post-capillary veins. This process plays a critical role in promoting tumorigenesis and metastasis. As a result, developing antiangiogenic agents has become an attractive strategy for tumor treatment. Sirtuin6 (SIRT6), a member of nicotinamide adenine (NAD+)-dependent histone deacetylases, regulates various biological processes, including metabolism, oxidative stress, angiogenesis, and DNA damage and repair. Some SIRT6 inhibitors have been identified, but the effects of SIRT6 inhibitors on anti-angiogenesis have not been reported. We have identified a pyrrole-pyridinimidazole derivative 8a as a highly effective inhibitor of SIRT6 and clarified its anti-pancreatic-cancer roles. This study investigated the antiangiogenic roles of 8a. We found that 8a was able to inhibit the migration and tube formation of HUVECs and downregulate the expression of angiogenesis-related proteins, including VEGF, HIF-1α, p-VEGFR2, and N-cadherin, and suppress the activation of AKT and ERK pathways. Additionally, 8a significantly blocked angiogenesis in intersegmental vessels in zebrafish embryos. Notably, in a pancreatic cancer xenograft mouse model, 8a down-regulated the expression of CD31, a marker protein of angiogenesis. These findings suggest that 8a could be a promising antiangiogenic and cancer therapeutic agent.
Psychological and Social Aspects of Vaccination Hesitancy—Implications for Travel Medicine in the Aftermath of the COVID-19 Crisis: A Narrative Review
Authors: Milionis Charalampos , Ilias Ioannis , Tselebis Athanasios , Pachi Argyro
Published: September 2023

Vaccines are an important tool of preventive medicine. Although organized vaccination programs have saved large populations from serious infectious diseases, there is a considerable part of the population who oppose vaccinations. In particular, anti-vaccination perceptions, among travelers to countries with endemic diseases, are a major public health concern. Although hesitancy towards vaccinations is not a novel phenomenon, it came back to the forefront during the fight against the COVID-19 pandemic. This review explores the etiology of anti-vaccination beliefs among travelers and draws conclusions about their impact on public health and society in general. For this purpose, a purposeful search for data on the causative factors of vaccine hesitancy and their impact on people’s health was conducted. A descriptive analysis of the findings and conclusions regarding possible implications in health policy and clinical practice are presented. A fear of side effects, lack of credence in the necessity of vaccines, and mistrust of medical authorities are important causative factors. Their interplay shapes hesitancy towards vaccines. However, anti-vaccination beliefs can also be an aspect of a more general unconventional stance of life. Health care professionals and organizations must be ready to tackle vaccine hesitancy by making the necessary interventions. Correcting misconceptions about vaccinations is a prerequisite for ensuring personal and public health, especially in the context of a pandemic or epidemic. Moreover, ensuring the efficacy and safety of vaccines, especially in cases of modern technology applications, is a fundamental factor in addressing people’s concerns about vaccines. For this purpose, medical authorities and organizations must provide accurate and clear information on vaccines so as to eliminate misinformation. Furthermore, clinicians should cultivate their communication skills in order to convey the appropriate messages to prospective recipients of vaccinations.
Particularities of Urinary Tract Infections in Diabetic Patients: A Concise Review
Authors: Confederat Luminita-Georgeta , Condurache Mihaela-Iustina , Alexa Raluca-Elena , Dragostin Oana-Maria
Published: September 2023

Diabetes mellitus is a chronic disease that, untreated or poorly controlled, can lead to serious complications, reducing life expectancy and quality. Diabetic patients are more likely to develop infections, including many common infections, but also pathognomonic ones such as emphysematous pyelonephritis, malignant otitis externa, mucormycosis and Fournier’s gangrene. Considering the fact that diabetic patients experience more frequently urinary tract infections (UTIs) with a worse prognosis than non-diabetic people, we conducted a review study based on data in the literature, following the particularities of UTIs in this group of patients, the risk factors, the mechanisms involved and the challenges in their management. The findings highlight that UTI in diabetic patients have some particularities, including a more frequent evolution to bacteremia, increased hospitalizations, and elevated rates of recurrence and mortality than non-diabetic patients. The possible risk factors identified seem to be female gender, pregnancy, older age, UTI in the previous six months, poor glycemic control and duration of diabetes. The mechanisms involved are related to glucosuria and bladder dysfunction, factors related to bacterial strains and host response. The bacterial strains involved in UTIs in diabetic patients and their antibiotic susceptibility profile are, with some exceptions, similar to those in non-diabetic people; however, the antimicrobial agents should be carefully chosen and the duration of the treatment should be as those required for a complicated UTI. The data related to the risk of developing UTIs in patients treated with SGLT-2 inhibitors, a new class of oral hypoglycaemic agents with cardiovascular and renal benefits, are controversial; overall, it was evidenced that UTIs occurred at the initiation of the treatment, recurrent infection was uncommon and the majority of UTIs responded to treatment with standard antibiotics. Moreover, interruption or discontinuation of SGLT-2 inhibitor as a result of UTI was rare and SGLT-2 inhibitors did not increase the risk of severe infections such as urosepsis and pyelonephritis.
Parameter Identification of the Fractional-Order Mathematical Model for Convective Mass Transfer in a Porous Medium
Authors: Pavlenko Ivan , Ochowiak Marek , Włodarczak Sylwia , Krupińska Andżelika , Matuszak Magdalena
Published: September 2023

Fractional calculus is an essential tool in studying new phenomena in hydromechanics and heat and mass transfer, particularly anomalous hydromechanical advection–dispersion considering the fractal nature of the porous medium. They are valuable in solving the urgent problem of convective mass transfer in a porous medium (e.g., membranes, filters, nozzles, convective coolers, vibrational prillers, and so on). Its solution allows for improving chemical engineering and technology workflows, refining process models for obtaining porous granular materials, realizing the convective cooling of granular and grain materials, and ensuring the corresponding apparatuses’ environmental safety. The article aims to develop a reliable convective mass transfer model for a porous medium and proposes a practical approach for its parameter identification. As a result, a general scientific and methodological approach to parameter identification of the fractional convective mass transfer model in a porous medium was proposed based on available experimental data. It mainly used Riemann–Liouville fractional time and coordinate derivatives. The comprehensive application of the Laplace obtained the corresponding general solution transform with respect to time and a coordinate, the Mittag-Leffler function, and specialized functions. Different partial solutions in various application case studies proved this solution. Moreover, the algorithm for practically implementing the developed approach was proposed to evaluate parameters for the considered model by evaluation data. It was reduced to the two-parameter model and justified by the available experimental data.
A Novel Synthesis of Quasi-Chebyshev Ultra-Wideband Bandpass Filter Using Nth Order Stub Loaded Coupled-Line Resonator
Authors: Abdul Rehman Muhammad , Khalid Sohail , Mushtaq Bilal , Uddin Mueen , Iqbal Jawaid , Abdelhaq Maha , Alsaqour Raed
Published: September 2023

This paper presents a novel synthesis of a quasi-Chebyshev Nth order stub-loaded coupled-line ultra-wideband bandpass filter. A unit element of a proposed filter topology consists of two short-circuited stubs loaded at the edges of coupled lines. A distributed equivalent circuit model of a proposed topology is extracted and used to acquire a generalized filtering function. The extracted filtering function is of rational form. The denominator of the filtering function causes a mismatch with Chebyshev type-I polynomials. For conventional narrowband filters, the denominator term can be neglected because of the close vicinity of band-edge frequencies; however, for the ultra-wideband filter response, the factor in the denominator cannot be neglected and hence requires a new mathematical procedure to compensate for the effect of the frequency-dependent term in the denominator. The electrical parameters are calculated using the proposed synthesis and used to design an ideal filter topology on ADS. To validate the proposed design procedure, fabrication is performed on a high-frequency substrate. The proposed filter is miniaturized in size and has good out-of-band performance. The simulated and measured results provide good agreement.
Alternative Approach to Design and Optimization of High-Q Ring Resonators for Membrane-Free Acoustic Sensors
Authors: Zheng Yongqiu , Chen Jiamin , Han Yuan , Bai Jiandong , Luo Yifan , Wang Yonghua , Xue Chenyang
Published: September 2023

Membrane-free acoustic sensors based on new principle and structure are becoming a research hotspot, because of many advantages, e.g., their wide bandwidth and high sensitivity. It is proposed that a membrane-free acoustic sensor employs a semi-buried optical waveguide ring resonator (SOWRR) as a sensing element. Using air as the upper cladding medium, the excited evanescent field in the air cladding medium would be modulated by acoustic wave. On this basis, the acoustic sensing model is established. Taking high Q factor and resonance depth as design criteria, the optimal design parameters are given. The optimal values of the air/SiO2: Ge/SiO2 waveguide resonator length and coupling spacing are obtained as 50 mm and 5.6 μm, respectively. The Q factor of the waveguide resonator of this size is as high as 8.33 × 106. The theoretical simulation indicates that the frequency response ranges from 1 Hz to 1.58 MHz and that the minimum detectable sound pressure is 7.48 µPa using a laser with linewidth of 1 kHz. Because of its advantages of wide bandwidth and high sensitivity, the membrane-free sensor is expected to become one of the most promising candidates for the next-generation acoustic sensor.
Recent Progress on Chelating Reagents in Flotation of Zinc Oxide Ores: A Review
Authors: Song Zhengyong , Wen Shuming , Han Guang , Feng Qicheng
Published: September 2023

Zinc oxide minerals (primarily smithsonite and hemimorphite) are important sources of Zn. Flotation is the most widely used method of enriching zinc oxide minerals for mineral processing. Chelating reagents have received extensive attention for the flotation of zinc oxide ores because of their high selectivity and stability. This paper systematically summarizes the selective separation performance and mechanisms of various chelating reagents as collectors, activators, and depressants in the flotation of zinc oxide ores. The types and mechanisms of chelating reagents in the flotation of zinc oxide ores are discussed, providing new ideas for the development of efficient flotation reagents for zinc oxide ores.
The Role of Nanofluids in Renewable Energy Engineering
Authors: Bhatti Muhammad Mubashir , Vafai Kambiz , Abdelsalam Sara I.
Published: September 2023

The phenomenon of nanofluid flows is intrinsically characterized by several scales and intricate physical processes [...]
Direct Evidence of Dynamic Metal Support Interactions in Co/TiO2 Catalysts by Near-Ambient Pressure X-ray Photoelectron Spectroscopy
Authors: Salusso Davide , Scarfiello Canio , Efimenko Anna , Pham Minh Doan , Serp Philippe , Soulantica Katerina , Zafeiratos Spyridon
Published: September 2023

The interaction between metal particles and the oxide support, the so-called metal–support interaction, plays a critical role in the performance of heterogenous catalysts. Probing the dynamic evolution of these interactions under reactive gas atmospheres is crucial to comprehending the structure–performance relationship and eventually designing new catalysts with enhanced properties. Cobalt supported on TiO2 (Co/TiO2) is an industrially relevant catalyst applied in Fischer−Tropsch synthesis. Although it is widely acknowledged that Co/TiO2 is restructured during the reaction process, little is known about the impact of the specific gas phase environment at the material’s surface. The combination of soft and hard X-ray photoemission spectroscopies are used to investigate in situ Co particles supported on pure and NaBH4-modified TiO2 under H2, O2, and CO2:H2 gas atmospheres. The combination of soft and hard X-ray photoemission methods, which allows for simultaneous probing of the chemical composition of surface and subsurface layers, is one of the study’s unique features. It is shown that under H2, cobalt particles are encapsulated below a stoichiometric TiO2 layer. This arrangement is preserved under CO2 hydrogenation conditions (i.e., CO2:H2), but changes rapidly upon exposure to O2. The pretreatment of the TiO2 support with NaBH4 affects the surface mobility and prevents TiO2 spillover onto Co particles.
Targeting Neutrophil Extracellular Traps for Stroke Prognosis: A Promising Path
Authors: Liaptsi Eirini , Merkouris Ermis , Polatidou Efthymia , Tsiptsios Dimitrios , Gkantzios Aimilios , Kokkotis Christos , Petridis Foivos , Christidi Foteini , Karatzetzou Stella , Karaoglanis Christos , Tsagkalidi Anna-Maria , Chouliaras Nikolaos , Tsamakis Konstantinos , Protopapa Maria , Pantazis-Pergaminelis Dimitrios , Skendros Panagiotis , Aggelousis Nikolaos , Vadikolias Konstantinos
Published: September 2023

Stroke has become the first cause of functional disability and one of the leading causes of mortality worldwide. Therefore, it is of crucial importance to develop accurate biomarkers to assess stroke risk and prognosis. Emerging evidence suggests that neutrophil extracellular trap (NET) levels may serve as a valuable biomarker to predict stroke occurrence and functional outcome. NETs are known to create a procoagulant state by serving as a scaffold for tissue factor (TF) and platelets inducing thrombosis by activating coagulation pathways and endothelium. A literature search was conducted in two databases (MEDLINE and Scopus) to trace all relevant studies published between 1 January 2016 and 31 December 2022, addressing the potential utility of NETs as a stroke biomarker. Only full-text articles in English were included. The current review includes thirty-three papers. Elevated NET levels in plasma and thrombi seem to be associated with increased mortality and worse functional outcomes in stroke, with all acute ischemic stroke, intracerebral hemorrhage, and subarachnoid hemorrhage included. Additionally, higher NET levels seem to correlate with worse outcomes after recanalization therapies and are more frequently found in strokes of cardioembolic or cryptogenic origin. Additionally, total neutrophil count in plasma seems also to correlate with stroke severity. Overall, NETs may be a promising predictive tool to assess stroke severity, functional outcome, and response to recanalization therapies.
Survivors Overcoming and Achieving Resiliency (SOAR): Mindful Eating Practice for Breast Cancer Survivors in a Virtual Teaching Kitchen
Authors: Huang Sherri , Riccardi Diane , Pflanzer Sonya , Redwine Laura S. , Gray Heewon L. , Carson Tiffany L. , McDowell Marc , Thompson Zachary , Hubbard Jesse J. , Pabbathi Smitha
Published: September 2023

The practice of mindful eating brings awareness to food choices, brings attention to the eating experience, and encourages selecting and preparing food that is both satisfying and nourishing. We examined mindful eating in breast cancer survivors following a 9-week, multidisciplinary virtual teaching kitchen intervention called Survivors Overcoming and Achieving Resiliency (SOAR). SOAR engaged participants through weekly cooking classes that also taught multiple domains of mindfulness. Participants (n = 102) were breast cancer survivors and completed the Mindful Eating Questionnaire (MEQ) prior to and after completion of the intervention. Linear regression analyses examined relationships between the aspects of mindful eating and body mass index (BMI). Wilcoxon (paired) rank sum tests evaluated the significance of the change in the MEQ total sum and subscales scores. A total of 102 participants completed both the pre- and post-intervention surveys. The mean change between the pre- and post-SOAR MEQ summary scores was 0.12 (sd = 0.30; Wilcoxon p-value = 0.0003). All MEQ subscale scores significantly increased with the exception of the distraction subscale. The MEQ summary scores increased for participants across both BMI stratifications. The SOAR teaching kitchen represents one of the first interventions that is tailored for breast cancer survivors and combines behavioral strategies from mindful eating training to nutritional knowledge and culinary medicine pedagogy in a virtual teaching kitchen. Further research is needed to examine whether mindful eating practices among cancer survivors result in sustainable healthy eating behaviors and food choices consistent with the cancer risk reduction guidelines.
Nutrition and Developmental Origins of Kidney Disease
Authors: Nguyen Long T. , Pollock Carol A. , Saad Sonia
Published: September 2023

The developmental programming hypothesis proposes that adverse environmental insults during critical developmental periods increase the risk of diseases later in life. The kidneys are deemed susceptible to such a process, although the exact mechanisms remain elusive. Many factors have been reported to contribute to the developmental origin of chronic kidney diseases (CKD), among which peri-gestational nutrition has a central role, affecting kidney development and metabolism. Physiologically, the link between malnutrition, reduced glomerular numbers, and increased blood pressure is key in the developmental programming of CKD. However, recent studies regarding oxidative stress, mitochondrial dysfunction, epigenetic modifications, and metabolic changes have revealed potential novel pathways for therapeutic intervention. This review will discuss the role of imbalanced nutrition in the development of CKD.
Mendelian Randomization Analysis Reveals Causal Effects of Polyunsaturated Fatty Acids on Subtypes of Diabetic Retinopathy Risk
Authors: Ren Shaojie , Xue Chen , Xu Manhong , Li Xiaorong
Published: September 2023

Polyunsaturated fatty acids (PUFAs) affect several physiological processes, including visual acuity, but their relationship with diabetic retinopathy (DR) remains elusive. The aim of this study was to determine whether PUFAs have a causal effect on DR. PUFAs- (total and omega-3 [FAw3] and omega-6 [FAw6] fatty acids and their ratio) and DR-associated single nucleotide polymorphisms derived from genome-wide association studies; sample sizes were 114,999 for fatty acids and 216,666 for any DR (ADR), background DR (BDR), severe non-proliferative DR (SNPDR), and proliferative DR (PDR). We hypothesized that the intra-body levels of PUFAs have an impact on DR and conducted a two-sample Mendelian randomization (MR) study to assess the causality. Pleiotropy, heterogeneity, and sensitivity analyses were performed to verify result reliability. High levels of PUFAs were found to be associated with reduced risk of both ADR and PDR. Moreover, FAw3 was associated with a decreased risk of PDR, whereas FAw6 demonstrated an association with lowered risks of both BDR and PDR. Our findings provide genetic evidence, for the first time, for a causal relationship between PUFAs and reduced DR risk. Consequently, our comprehensive MR analysis strongly urges further investigation into the precise functions and long-term effects of PUFAs, FAw3, and FAw6 on DR.
Nutritional Status Indices and Monoclonal Gammopathy of Undetermined Significance Risk in the Elderly Population: Findings from the National Health and Nutrition Examination Survey
Authors: Li Linfeng , Wu Mengrui , Yu Zhengyu , Niu Ting
Published: September 2023

Objective: Although several studies have found dietary intake is related to multiple myeloma (MM) and its precursor status risks, the role of one’s nutritional status has been ignored and its role in plasma cell neoplasm development is still unclear. This study aimed to explore the relationship between various clinical indices of nutritional status and the risk of monoclonal gammopathy of undetermined significance (MGUS) in the population. Methods: We selected 9520 participants from the NHANES III and NHANES 1999–2004 studies. Controlling nutritional status index (CONUT), prognostic nutritional index (PNI), geriatric nutritional risk index (GNRI) and body mass index (BMI) were calculated as indices of nutritional status of the participants. Associations between nutritional indices and MGUS were investigated using multiple logistic regression, subgroup analysis, and an RCS model. Results: In our study, 266 participants had MGUS, with a prevalence of 2.79%. This study found that CONUT and PNI identified populations with poor nutritional status and had a significant positive correlation with the risk of MGUS. In multivariate logistic regression, compared with the lower CONUT score (<3) group, the OR for the group with higher scores (≥3) was 1.805 (95%CI: 1.271, 2.564). Compared with the lowest quartile group, the highest quartile PNI score group had an OR of 0.509 (95%CI: 0.290, 0.896). GNRI had no significant correlation with the risk of MGUS, with an OR of 0.737 (95%CI: 0.443, 1.227). Conclusion: This study found that older adults with CONUT and PNI scores indicating poorer nutrition had a higher risk of MGUS.
An Investigation of the Protein Quality and Temporal Pattern of Peripheral Blood Aminoacidemia following Ingestion of 0.33 g·kg−1 Body Mass Protein Isolates of Whey, Pea, and Fava Bean in Healthy, Young Adult Men
Authors: Kozior Marta , Davies Robert W. , Amigo-Benavent Miryam , Fealy Ciaran , Jakeman Philip M.
Published: September 2023

An increase in the intake of legumes is recommended in the promotion of plant-sourced (PSP) rather than animal-sourced (ASP) protein intake to produce a more sustainable diet. This study evaluated the quality of novel PSP isolates from pea (PEA) and fava bean (FAVA) and an ASP isolate of whey (WHEY) and compared the magnitude and temporal pattern of peripheral arterial aminoacidemia following ingestion of 0.33 g·kg−1 body mass of protein isolate in healthy young adult men (n = 9). Total indispensable amino acids (IAA) comprised 58% (WHEY), 46% (PEA), and 42% (FAVA) of the total amino acid (AA) composition, with the ingested protein providing 108% (WHEY), 77% (PEA), and 67% (FAVA) of the recommended per diem requirement of IAA. Reflecting the AA composition, the area under the curve (∆AUC0-180), post-ingestion increase in total IAA for WHEY was 41% (p < 0.001) and 57% (p < 0.001) greater than PEA and FAVA, respectively, with PEA exceeding FAVA by 28% (p = 0.003). As a sole-source, single-dose meal-size serving, the lower total IAA for PEA and FAVA would likely evoke a reduced post-prandial anabolic capacity compared to WHEY. Incorporated into a food matrix, the promotion of PSP isolates contributes to a more sustainable diet.
Numerical Mechanistic Modelling of Drug Release from Solvent-Removal Zein-Based In Situ Gel
Authors: Senarat Setthapong , Pornsawad Pornsarp , Lertsuphotvanit Nutdanai , Østergaard Jesper , Phaechamud Thawatchai
Published: September 2023

The development of effective drug delivery systems remains a focus of extensive research to enhance therapeutic outcomes. Among these, in situ forming gels (ISG) have emerged as a promising avenue for controlled drug release. This research focuses on the mathematical modeling of levofloxacin HCl (Lv) release from zein-based ISG using the cup method, aiming to mimic the environment of a periodontal pocket. The drug release behavior of the ISGs was investigated through experimental observations and numerical simulations employing forward and central difference formula. Notably, the experimental data for drug release from the 20% w/w zein-based ISG formulations closely aligned with the simulations obtained from numerical mechanistic modeling. In summary, 20% w/w zein-based ISG formulations demonstrated nearly complete drug release with the maximum drug concentration at the edge of the matrix phase values consistently around 100–105%, while 25% w/w zein-based ISG formulations exhibited somewhat lower drug release extents, with values ranging from 70–90%. Additionally, the rate of drug transport from the polymer matrix to the external phase influenced initial release rates, resulting in a slower release. The utilization of glycerol formal as a solvent extended drug release further than dimethyl sulfoxide, thanks to denser matrices formed by high-loading polymers that acted as robust barriers to solvent removal and drug diffusion. Furthermore, UV-vis imaging was utilized to visualize the matrix formation process and solvent diffusion within the ISGs. The imaging results offered valuable insights into the matrix formation kinetics, controlled drug release mechanisms, and the influence of solvent properties on drug diffusion. The combination of mathematical modeling and experimental visualization provides a comprehensive understanding of drug release from zein-based ISGs and offers a foundation for tailored drug delivery strategies.