bluuebunny/crossref_metadata_2025 huggingface dataset:
The effectiveness of self-advocacy videos to inform enablers about the support needs of students with vision impairment
Authors: Swain Gaby , Waddington Jonathan
Published: November 2020

The aim of this project was to understand how enablers (e.g., teaching assistants, paraprofessionals and support workers) access and use information about students with vision impairment (VI) to support them in specialist education. The one-page profile is used widely as a tool for learner-centred planning and information, and is generally seen to be effective and accessible. However, more recent studies have demonstrated that video is an extremely effective medium for training and support in a range of settings. We investigated whether student self-advocacy video clips would be an effective and accessible medium for presenting information about students’ support needs. This study took the form of a pre- and post-questionnaire. The aim of the pre-questionnaire was to gauge current levels of enabler confidence and assess the effectiveness of existing methods of accessing learner information. A total of 15 enablers were given this questionnaire to complete. Six students (aged 11–17 years) with VI and additional complex support needs were then each supported to develop a short video in which they expressed their support needs in an education setting. The enablers were asked to watch the video clips, and complete the post-questionnaire to assess the impact these videos had on their confidence and understanding. Twelve out of the 15 enablers returned the second questionnaire. The questionnaire results demonstrated that current methods for accessing learner information were not wholly effective. Enablers found video to be a desirable and accessible format for presenting learner information. Participants found the medium of video to significantly aid retention and recollection of student information. From the findings, it emerged that one-page profiles remained the preferred single method of accessing student information. However, overall, we found that enablers favoured a multi-method approach to presenting and accessing learner information that was dependent on time and context.
Comparison of intra-wound drainage tubes after cardiac surgery: Blake drains versus Multichannel drains
Authors: Hirata Yuichiro , Tayama Eiki , Ueda Tomohiro , Uchiyama Hikaru , Onzuka Tatsushi , Furukawa Kojiro , Morita Shigeki
Published: November 2020

Background:

Blake and Multichannel drains have been used in our department. Although both are made up of silicone, they differ in structure. We investigated the drainage effects of these two types of drains and the factors related to their occlusion.

Methods:

We enrolled 100 consecutive cases (50 using Blake drains and 50 using Multichannel drains) of cardiovascular surgery performed in our department from July 2017 to April 2018. The formation of thrombi in the groove and tube of the drains was evaluated in each case. The tube portion was checked for the presence of occlusion, and the groove portion was examined for the number and ratio of thrombi formed in the grooves.

Results:

The clot formation rate in the groove part was slightly higher in the Multichannel cases than in the Blake cases. In addition, analysis within the Multichannel cases revealed that the thrombus formation rate between the catheter lumen and the three grooves (without the catheter lumen) was significantly different, with the highest groove clot formation rate occurring in the catheter lumen. Out of 34 cases of occlusions, there were 26 cases (52%) of Multichannel drains, and only 8 cases (16%) of Blake drains ( p < 0.01). A multiple logistic regression analysis revealed that the most important contributory factor in tube obstruction was the drain type.

Conclusions:

The catheter lumen of the Multichannel drain was more susceptible to thrombus formation than the groove. The tube part of the Multichannel drain was more prone to occlusion than that of the Blake drain.
Evaluation of the biogas potential of agricultural biomass waste for energy applications in Greece: A case study of the western Greece region
Authors: Moustakas Konstantinos , Sotiropoulos Dimitrios , Vakalis Stergios
Published: November 2020

Agricultural biomass can be best described as the organic matter residues from farming that remain within the fields after harvesting, along with tree trimmings. From the overall Greek Energy Balance, only a small fraction consists of biomass and this has been the main driving force behind this study. Due to the numerous ongoing agricultural activities, western Greece was selected as an ideal area for a case study. As a second step, the aim was to investigate the feasibility of the current anaerobic digestion plants to utilize the total biomass as feedstock. An additional scope to provide certifiable proof of the essential rural biomass assets available. Information on the potential of agricultural biomass is provided, with a focus on the performance specifications and the social advantages, but also the soil added substances and the produced biofuels. Subsequently, two options for waste management were discussed to illustrate the possibility of generating energy. The anaerobic digestion plants available in western Greece are illustrated in detail and the yearly rate of the main agrarian biomass is evaluated to be 715,080 tons. Arable crops, mechanical plants and tree trimming are recorded as the noteworthy sources. It is estimated that the proposed anaerobic digestion system will handle the entire amount of biomass and deliver max per year electricity 775 GWh and thermal energy 1.119 GWh.
Matches Made With Information: Fitting Measurement Models to Adult Attachment Data
Authors: Sun Tianjun , Fraley R. Chris , Drasgow Fritz
Published: November 2020

Many self-report inventories in social/personality psychology are developed and scored using dominance-based assumptions. Specifically, they assume that the relationship between item endorsement and the latent trait is monotonically increasing; thus, individuals with high standings on the trait would be likely to endorse all items. It is possible, however, that the item response process for these inventories follows an ideal point process in which respondents only endorse items that best describe them, leading to nonmonotonic relations between item responses and latent traits. This research examined whether the item response process underlying the Experiences in Close Relationships–Revised—a commonly used self-report measure of adult attachment styles—is best understood as a dominance or ideal point process. Study 1 showed that the ideal point model provided a good account of the response process and provided better interpretability for the full trait continuum than a dominance model. Importantly, people who were the most insecure were the most likely to be scored differently under these two item response models. In Study 2, the association between attachment anxiety and subjective well-being scores was higher using ideal point than dominance-based scoring, and this was especially the case among subsets of people who were highly insecure. Study 3 demonstrated a similar pattern using simulation data. In summary, when dominance-based methods are used to measure adult attachment, people who are extremely insecure may be assessed in suboptimal ways.
Global and Diverse Markers: Challenges and Opportunities That Await Us in 2045
Authors: Lipschultz Jeremy Harris
Published: November 2020

The Post-World War II era, Journalism Quarterly pedagogical research interests, and a 21st century drive to be global and rigorous helped define the emergence of Journalism and Mass Communication Educator during a time of technological, political, social, and economic disruption. The journalism field and academic programs face continuing challenges and opportunities during the next 25 years.
Intellectual emancipation as minimal humanism – The relevance of Jacques Rancière in business school teaching
Authors: Kogut Mads , Sørensen Thaning Morten , Birksted Niklas
Published: November 2020

How might emancipatory teaching practices look like in the context of the business school, when the meaning of the subject of emancipation, the human being, has become unsettled? Our philosophical essay addresses this question by excavating Jacques Rancière’s conception of intellectual emancipation and showing its practical relevance for experiments with emancipatory teaching in a business school environment. Speaking from within a tradition where the meaning of human is irrevocably unsettled, Rancière, remarkably, still insists on an essential link between emancipation and humanism – although in a minimal version. First, we show why and how Rancière’s analyses of emancipation are united by the common concern to affirm such a minimal humanism. Thereafter, we describe how three features sets intellectual emancipation apart from social and aesthetic emancipation and makes it pertinent to take intellectual emancipation to school: The possibility and intention to emancipate others, the acknowledgement of the constructive role of reason herein, and the significance of teacher authority in doing so. Lastly, we move beyond and problematize Rancière’s clear conceptual account of intellectual emancipation by extracting three heuristic pedagogical devices from it and by recounting their confrontation with the messy details of our teaching practice at the business school.
Barriers, access and management of paediatric epilepsy with telehealth
Authors: Gali Kari , Joshi Sucheta , Hueneke Sarah , Katzenbach Alexis , Radecki Linda , Calabrese Trisha , Fletcher Linda , Trandafir Cristina , Wilson Carey , Goyal Monisha , Wusthoff Courtney J , Le Pichon Jean-Baptiste , Corvalan Rhonda , Golson April , Hardy Jessica , Smith Michael , Cook Elizabeth , Bonkowsky Joshua L
Published: November 2020

Access to paediatric neurology care is complex, resulting in significant wait times and negative patient outcomes. The goal of the American Academy of Pediatrics National Coordinating Center for Epilepsy's project, Access Improvement and Management of Epilepsy with Telehealth (AIM-ET), was to identify access and management challenges in the deployment of telehealth technology. AIM-ET organised four paediatric neurology teams to partner with primary-care providers (PCP) and their multidisciplinary teams. Telehealth visits were conducted for paediatric epilepsy patients. A post-visit survey assessed access and satisfaction with the telehealth visit compared to an in-person visit. Pre/post surveys completed by PCPs and neurologists captured telehealth visit feasibility, functionality and provider satisfaction. A provider focus group assessed facilitators and barriers to telehealth. Sixty-one unique patients completed 75 telehealth visits. Paired t-test analysis demonstrated that telehealth enhanced access to epilepsy care. It reduced self-reported out-of-pocket costs ( p<0.001), missed school hours ( p<0.001) and missed work hours ( p<0.001), with 94% equal parent/caregiver satisfaction. Focus groups indicated developing and maintaining partnerships, institutional infrastructure and education as facilitators and barriers to telehealth. Telehealth shortened travelling distance, reduced expenses and time missed from school and work. Further, it provides significant opportunity in an era when coronavirus disease 2019 limits in-person clinics.
Extensor Retinaculum Reconstruction of the Distal Radioulnar Joint in Adolescents
Authors: Bauer Andrea S. , Lee Stella J. , Smith Michael D. , Bae Donald S. , Waters Peter M.
Published: November 2020

Background

This study characterizes the outcomes and complications of surgical reconstruction of distal radioulnar joint (DRUJ) instability using the extensor retinaculum (Herbert sling). Our hypothesis was that extensor retinaculum reconstruction is a reliable method of DRUJ stabilization in adolescents.

Methods

This was a retrospective study of pediatric patients treated surgically using the Herbert sling for DRUJ instability at a single institution. We identified 22 subjects who underwent surgery at an average of 16.2 years of age (range, 12-18 years). Medical records and available imaging were reviewed for all subjects, and patients were contacted to participate in the prospective completion of the Quick Disabilities of the Arm, Shoulder, and Hand (QuickDASH) questionnaire.

Results

Preoperative symptoms were more commonly pain (95%) than feelings of DRUJ instability (45%), although 100% had instability on physical examination. Eight (36%) patients demonstrated limited supination preoperatively. Twenty-one subjects (95%) noted prior injury to that wrist, 15 of which were distal radius fractures. Surgery consisted of stabilization of the DRUJ using extensor retinaculum, in concert with other procedures to address all potential causes of wrist pain. Postoperatively, DRUJ stability was maintained in 21 of 22 subjects. Of the 12 patients who provided functional outcome scores, median QuickDASH score was 7.6 (range, 0-45).

Conclusions

Distal radioulnar joint instability in adolescents is often preceded by fracture of the distal radius. Surgeons must maintain a high level of suspicion to appropriately diagnose DRUJ instability, which is often not an isolated pathoanatomical problem. The Herbert sling technique using extensor retinaculum can successfully confer DRUJ stability in this population.
Taking crime guns seriously: A socio-material perspective
Authors: Goldsmith Andrew , Halsey Mark , Bright David
Published: November 2020

This article argues that guns, as objects used in and for crime, have received insufficient criminological attention. It proposes a socio-material perspective for taking crime guns seriously as material agents in the ways many serious crimes are planned and executed. Drawing in part upon affordance theory, the perspective links the ‘objective’ physical properties of guns to their allure and take up for the purposes of carrying out crime. Guns are powerful organising objects in the commission of crime, it is argued, capable of provoking as well as enabling a range of threatening and harmful activities. The perspective is developed drawing upon interview data from a large qualitative study of convicted gun criminals. These data enable the notion of materiality to be considered at different stages of criminal career, particularly prior to first criminal gun use through to enforced or voluntary desistance. The article concludes with a consideration of policy options suggested by the socio-material perspective. In a post-Covid 19 world in which guns have gained greater salience in many countries, it is argued that the need to ‘dematerialise’ gun attraction and use has never been greater.
There Is No Primacy Effect in Interpersonal Perception: A Series of Preregistered Analyses Using Judgments of Actual Behavior
Authors: Wiedenroth Anne , Wessels Nele M. , Leising Daniel
Published: November 2020

First impressions are commonly assumed to be particularly important: Information about a person that we obtain early on may shape our overall impression of that person more strongly than information obtained later. In contrast to previous research, the present series of preregistered analyses uses actual person judgment data to investigate this so-called primacy effect: Perceivers ( N = 1,395) judged the videotaped behavior of target persons ( N = 200) in 10 different situations. Separate subsamples of about 200 perceivers each were used in moving from exploratory to increasingly confirmatory analyses. Contrary to our expectations, no primacy effect was found. Instead, judgments of the targets in later situations were more strongly associated with overall impressions, indicating an acquaintance effect. Relying on early information seems unreasonable when more comprehensive information is readily available. Early information may, however, affect perceivers’ behavioral reactions to the targets and thus their future interactions, if such interactions are possible.
Prediction of pulmonary hypertension in older adults based on vital capacity and systolic pulmonary artery pressure
Authors: Wernhart Simon , Hedderich Jürgen
Published: November 2020

Objective

Right heart catheterization (RHC) is associated with a higher procedural risk in older adults, but non-invasive estimation of pulmonary hypertension (PH) is a challenge. We aimed to elaborate a non-invasive prediction model to estimate PH.

Methods and design

We retrospectively analysed 134 older adults (70.0 years ±12.3; 44.9% males) who reported to our clinic with unclear dyspnea between 01/2015 and 01/2020 and had received RHC as a part of their diagnostic workup. Lung function testing, analysis of blood gas samples, 6 min walk distance and echocardiography were performed within 24 hours of RHC.

Main outcome measures

In a stepwise statistical approach by using an in/exclusion algorithm (using the AIC criterion) we analysed non-invasive parameters to test their value in predicting PH (defined as mean pulmonary artery pressure, PAmean, >25mmHg). Discrimination capability of the final model was measured by the AUC (area under curve) from an ROC (receiver operating characteristics) analysis.

Results

We yielded a sensitivity of 87.2% and a specificity of 62.5% in a combinatorial logistical model with systolic pulmonary artery pressure (sPAP) and forced vital capacity (VCmax), the discrimination index was 86.7%. The odds ratios for an increase of 10 mmHg of sPAP were 2.99 (2.08–4.65) and 1.86 (1.11–3.21) for a 1 l decrease in VCmax. On their own, VCmax proved to be specific (83.3%), while sPAP was a sensitive (79.1%) predictor for PH.

Conclusions

We provide a combinatorial model to predict PH from sPAP and VCmax in older adults, which may help to avoid invasive procedures.
Data capitalism and the counter futures of ethics in artificial intelligence
Authors: Dixon-Román Ezekiel , Parisi Luciana
Published: November 2020

Ethics in data science and artificial intelligence have gained broader prominence in both scholarly and public discourse. Much of the scholarly engagements have often been based on perspectives of transparency, politics of representation, moral ethical norms, and refusal. In this article, while the authors agree that there is a problem with the universal model of technology, they argue that what these perspectives do not address is the postcolonial epistemology of the machine. Drawing from Mark Fisher’s science fiction capital, it is posited that data capitalism doesn’t rely on data as a given, but on what data can become; it operates in the future as much as the calculation of probabilities coincides with the predictive extraction of surplus value. The authors argue that in order to address ethical and sociopolitical concerns in artificial intelligence, technosocial systems must be understood in data capitalism. After discussing what they characterize as the three paradigms of prediction, the authors point toward the transformative potential of temporal structures and indeterminacies in automated self-regulating systems. They argue therefore that assumptions of technological determinism that are found in debates about the reproduction of biases in systems of predictive intelligence has nothing to do with the technical machine, but is rather the result of a continuous re-territorialization of the technosocial possibilities of re-inventing epistemological paradigms outside the framework of colonial capital.
Concussion in collegiate athletics: A link to academic achievement
Authors: Walter Alexa E , Kaschak Christian , Sebastianelli Wayne , Arnett Peter , Slobounov Semyon M
Published: November 2020

Context

After concussion, many different symptoms can occur and persist that can affect daily functioning. Many of these symptoms could have implications in an athlete’s ability to return to academics. Unlike return to play, return to academics is less studied and less regulated. There is little research examining the effects of concussion on grade point average (GPA) and results have been inconsistent.

Objective

To examine the effects of concussion on college GPA.

Design

Retrospective observational study

Setting

University laboratory

Participants

Division I athletes after their first concussion (n = 26) and Division I athletes without a history of concussion (n = 30).

Main Outcome Measures

GPA and demographic information was obtained for the semester before injury, the semester of injury, and the semester after injury.

Results

Statistical analysis using generalized linear mixed model analysis revealed a significant interaction ( p < 0.05) of group (concussion vs. control) by time, with the concussed group having a significant decrease in GPA from semester before injury to semester of injury, and a significant main effect for sex ( p < 0.05) with females having higher GPAs than males.

Conclusions

Since the cognitive demands of academics can potentially exacerbate symptomology of concussion, the identification of students at risk for difficulties is critical.
Innovation and Sustainable Development: Does Aid and Political Instability Impede Innovation?
Authors: Nadeem Muhammad Athar , Liu Zhiying , Ali Haji Suleman , Younis Amna , Bilal Muhammad , Xu Yi
Published: November 2020

Sound innovation capabilities help the nations not only to capture bigger market shares but also to sustain long-term economic growth. Innovation is of vital importance at all stages of a country’s development as it promotes productivity, value creation, employment, economic growth, and sustainability. Several factors can affect the innovation activities of a country. For example, peaceful and stable environment, effective macroeconomic designs, sound institutional quality, and efficient utilization of resources are of great significance for a country to nourish economic, business, and market activities. Applying the Auto Regressive Distributive Lag approach to cointegration, this study investigates the short- and long-run impacts of aid, political instability, and terrorism upon the innovation of a laggard economy, namely, Pakistan. Our findings reveal that aid, political instability, and terrorism all have adverse impacts on innovation. Results across robustness checks remain the same. This study is of strong policy implications for policymakers, governments and opposition parties, and security and intelligence agencies to develop sound macroeconomic designs and policies, bring harmony for political stability, and curb terrorism, respectively.
Exposing the legal and bureaucratic underpinnings of gentrification: Municipal property transfers through alley closures in Washington, DC
Authors: Summer Rebecca
Published: November 2020

Scholars acknowledge that property ownership is fundamental to gentrification and that the privatization of public property can exacerbate market-based speculation on urban land. However, few have closely examined how the transfer of municipal property occurs. This article examines the political, legal, and bureaucratic preconditions for the acquisition of municipal land, which underpins gentrification. Drawing on the example of municipal alley closures in Washington, D.C. during the 1970s, this article traces how private developers acquired public land in downtown neighborhoods like the West End. It also traces how residents tried to oppose this acquisition of public property. In following West End residents’ failed attempts to retain control over the use and value of land in their neighborhood, this article exposes the legal and bureaucratic barriers that ordinary urban citizens face in interrupting the processes that contribute to gentrification.
Many Labs 5: Registered Multisite Replication of the Tempting-Fate Effects in Risen and Gilovich (2008)
Authors: Mathur Maya B. , Bart-Plange Diane-Jo , Aczel Balazs , Bernstein Michael H. , Ciunci Antonia M. , Ebersole Charles R. , Falcão Filipe , Ashbaugh Kayla , Hilliard Rias A. , Jern Alan , Kellier Danielle J. , Kessinger Grecia , Kolb Vanessa S. , Kovacs Marton , Lage Caio Ambrosio , Langford Eleanor V. , Lins Samuel , Manfredi Dylan , Meyet Venus , Moore Don A. , Nave Gideon , Nunnally Christian , Palinkas Anna , Parks Kimberly P. , Pessers Sebastiaan , Ramos Tiago , Rudy Kaylis Hase , Salamon Janos , Shubella Rachel L. , Silva Rúben , Steegen Sara , Stein L. A. R. , Szaszi Barnabas , Szecsi Peter , Tuerlinckx Francis , Vanpaemel Wolf , Vlachou Maria , Wiggins Bradford J. , Zealley David , Zrubka Mark , Frank Michael C.
Published: November 2020

Risen and Gilovich (2008) found that subjects believed that “tempting fate” would be punished with ironic bad outcomes (a main effect), and that this effect was magnified when subjects were under cognitive load (an interaction). A previous replication study (Frank & Mathur, 2016) that used an online implementation of the protocol on Amazon Mechanical Turk failed to replicate both the main effect and the interaction. Before this replication was run, the authors of the original study expressed concern that the cognitive-load manipulation may be less effective when implemented online than when implemented in the lab and that subjects recruited online may also respond differently to the specific experimental scenario chosen for the replication. A later, large replication project, Many Labs 2 (Klein et al. 2018), replicated the main effect (though the effect size was smaller than in the original study), but the interaction was not assessed. Attempting to replicate the interaction while addressing the original authors’ concerns regarding the protocol for the first replication study, we developed a new protocol in collaboration with the original authors. We used four university sites ( N = 754) chosen for similarity to the site of the original study to conduct a high-powered, preregistered replication focused primarily on the interaction effect. Results from these sites did not support the interaction or the main effect and were comparable to results obtained at six additional universities that were less similar to the original site. Post hoc analyses did not provide strong evidence for statistical inconsistency between the original study’s estimates and our estimates; that is, the original study’s results would not have been extremely unlikely in the estimated distribution of population effects in our sites. We also collected data from a new Mechanical Turk sample under the first replication study’s protocol, and results were not meaningfully different from those obtained with the new protocol at universities similar to the original site. Secondary analyses failed to support proposed substantive mechanisms for the failure to replicate.
Many Labs 5: Registered Replication of LoBue and DeLoache (2008)
Authors: Lazarević Ljiljana B. , Purić Danka , Žeželj Iris , Belopavlović Radomir , Bodroža Bojana , Čolić Marija V. , Ebersole Charles R. , Ford Máire , Orlić Ana , Pedović Ivana , Petrović Boban , Shabazian Ani N. , Stojilović Darko
Published: November 2020

Across three studies, LoBue and DeLoache (2008) provided evidence suggesting that both young children and adults exhibit enhanced visual detection of evolutionarily relevant threat stimuli (as compared with nonthreatening stimuli). A replication of their Experiment 3, conducted by Cramblet Alvarez and Pipitone (2015) as part of the Reproducibility Project: Psychology (RP:P), demonstrated trends similar to those of the original study, but the effect sizes were smaller and not statistically significant. There were, however, some methodological differences (e.g., screen size) and sampling differences (the age of recruited children) between the original study and the RP:P replication study. Additionally, LoBue and DeLoache expressed concern over the choice of stimuli used in the RP:P replication. We sought to explore the possible moderating effects of these factors by conducting two new replications—one using the protocol from the RP:P and the other using a revised protocol. We collected data at four sites, three in Serbia and one in the United States (total N = 553). Overall, participants were not significantly faster at detecting threatening stimuli. Thus, results were not supportive of the hypothesis that visual detection of evolutionarily relevant threat stimuli is enhanced in young children. The effect from the RP:P protocol ( d = −0.10, 95% confidence interval = [−1.02, 0.82]) was similar to the effect from the revised protocol ( d = −0.09, 95% confidence interval = [−0.33, 0.15]), and the results from both the RP:P and the revised protocols were more similar to those found by Cramblet Alvarez and Pipitone than to those found by LoBue and DeLoache.
ZLM-7 inhibits the occurrence and angiogenesis of breast cancer through miR-212-3p/Sp1/VEGFA signal axis
Authors: Li Xuan , Zou Zi-Zheng , Wen Min , Xie Yuan-Zhu , Peng Kun-Jian , Luo Tiao , Liu Su-You , Gu Qin , Li Ji-Jia , Luo Zhi-Yong
Published: November 2020

Background

Breast cancer (BC) is a common malignant tumor with poor prognosis. Angiogenesis is related to the growth and progression of solid tumors and associated with prognosis. ZLM-7, SP1, VEGFA and miR-212-3p were associated with BC angiogenesis and proliferation, however the detailed mechanism was not clear. This study aimed to reveal the regulatory mechanism of angiogenesis of BC.

Methods

BC cell lines were treated with 10 nM ZLM-7 for 8 h. We detected protein expression level by western blot and RNA expression level by qRT-PCR. Overexpression or inhibition of miR-212-3p is performed using miR-212-3p mimics or miR-212-3p inhibitor, Sp1 overexpression using pcDNA3.1 vector. Angiogenesis was analyzed by co-culturing BC cell lines and HUVEC cells. To evaluate regulatory relationship between miR-212-3p and Sp1, dual luciferase assay was performed. Besides, the direct interaction between Sp1 and VEGFA was analyzed by ChIP. Migration and invasion were analyzed by transwell assay and proliferation was detected by clone formation assay. In mice xenograft model developed using BC cells, we also detected angiogenesis marker CD31 through immunohistochemistry.

Results

ZLM-7 up-regulated miR-212-3p and inhibited invasion, migration, proliferation and angiogenesis of BC, while miR-212-3p inhibitor antagonized such effects. Binding sequence was revealed between miR-212-3p and Sp1, and expression of Sp1 was inhibited by miR-212-3p on both protein and mRNA level. Sp1 could interact with VEGFA and promoted its expression. Overexpression of miR-212-3p inhibited migration, invasion, proliferation and angiogenesis of BC cell lines, while Sp1 overexpression showed the opposite effect and could antagonize these effects of miR-212-3p overexpression. ZLM-7 decreased VEGFA expression, which was rescued by co-transfection with miR-212-3p inhibitor. Similar, ZLM-7 could inhibit tumor growth and angiogenesis through the miR-212-3p/Sp1/VEGFA axis in vivo.

Conclusions

ZLM-7 could directly up-regulate miR-212-3p in BC. MiR-212-3p could inhibit VEGFA expression through Sp1, thereby inhibiting angiogenesis and progression of BC.
Characterizing glycosyltransferases by a combination of sequencing platforms applied to the leaf tissues of Stevia rebaudiana
Authors: Zhang Shaoshan , Liu Qiong , Lyu Chengcheng , Chen Jinsong , Xiao Renfeng , Chen Jingtian , Yang Yunshu , Zhang Huihui , Hou Kai , Wu Wei
Published: November 2020

Background

Stevia rebaudiana (Bertoni) is considered one of the most valuable plants because of the steviol glycosides (SGs) that can be extracted from its leaves. Glycosyltransferases (GTs), which can transfer sugar moieties from activated sugar donors onto saccharide and nonsaccharide acceptors, are widely distributed in the genome of S. rebaudiana and play important roles in the synthesis of steviol glycosides.

Results

Six stevia genotypes with significantly different concentrations of SGs were obtained by induction through various mutagenic methods, and the contents of seven glycosides (stevioboside, Reb B, ST, Reb A, Reb F, Reb D and Reb M) in their leaves were considerably different. Then, NGS and single-molecule real-time (SMRT) sequencing were combined to analyse leaf tissue from these six different genotypes to generate a full-length transcriptome of S. rebaudiana. Two phylogenetic trees of glycosyltransferases (SrUGTs) were constructed by the neighbour-joining method and successfully predicted the functions of SrUGTs involved in SG biosynthesis. With further insight into glycosyltransferases (SrUGTs) involved in SG biosynthesis, the weighted gene co-expression network analysis (WGCNA) method was used to characterize the relationships between SrUGTs and SGs, and forty-four potential SrUGTs were finally obtained, including SrUGT85C2, SrUGT74G1, SrUGT76G1 and SrUGT91D2, which have already been reported to be involved in the glucosylation of steviol glycosides, illustrating the reliability of our results.

Conclusion

Combined with the results obtained by previous studies and those of this work, we systematically characterized glycosyltransferases in S. rebaudiana and forty-four candidate SrUGTs involved in the glycosylation of steviol glucosides were obtained. Moreover, the full-length transcriptome obtained in this study will provide valuable support for further research investigating S. rebaudiana.
Rapid identification, capsular typing and molecular characterization of Streptococcus pneumoniae by using whole genome nanopore sequencing
Authors: Garcia-Garcia S. , Perez-Arguello A. , Henares D. , Timoneda N. , Muñoz-Almagro C.
Published: November 2020

Background

Whole genome sequencing has emerged as a useful tool for identification and molecular characterization of pathogens. MinION (Oxford Nanopore) is a real-time third generation sequencer whose portability, affordability and speed in data production make of it an attractive device for whole genome sequencing. The objective of this study is to evaluate MinION sequencer for pathogen identification and molecular characterization of Streptococcus pneumoniae isolated at a children’s Hospital. Whole genome sequencing of 32 Streptococcus pneumoniae invasive isolates, previously characterized by standard methods (Quellung reaction, Multiplex PCR and Sanger-MLST), were performed. DNA was extracted using ZymoBIOMICS DNA Microprep kit. Quantification and purity of DNA was assessed by Qubit and Nanodrop, respectively. Library preparation was performed using the Rapid Barcoding Kit. Real-time workflow EPI2ME platform “What’s it in my pot” was used for species identification. Fast5 sequences were converted into FASTQ by Albacore software. Reads were assembled using CANU software. PathogenWatch, genomic epidemiology and pubmlst online tools were used for capsular typing and/or whole genome-MLST profile.

Results

Rapid identification of Streptococcus pneumoniae was achieved by “What’s in my pot”. Capsular typing was correctly assigned with PathogenWatch in all 32 isolates at serogroup level and 24 at serotype level. Whole genome-MLST results obtained by genomic epidemiology and pubmlst were consistent with double locus variant clonal complex obtained by Sanger-MLST in 31 isolates.

Conclusion

MinION sequencer provides a rapid, cost-effective and promising pathway for performing WGS by a pocked-sized device for epidemiological purposes but improving its sequencing accuracy will make it more appealing to be used in clinical microbiology laboratories.
Clinical and radiographic characteristics, management and short-term outcomes of patients with COVID-19 in Wenzhou, China
Authors: Hong Liang , Ye Enling , Sun Gangqiang , Wang Xiaoyang , Zhang Shengguo , Wu Yanghe , Xie Xiangao , Xia Shichun , Zheng Xudong , Dong Ling , Cai Fujing , Lou Xixian , Zhao Renguo , Hu Yongqi , Ruan Zhanwei , Ding Jiguang , Sun Qingfeng
Published: November 2020

Background

Coronavirus disease 2019 (COVID-19) is an emerging viral disease. Here, we report the clinical features, management, and short-term outcomes of COVID-19 patients in Wenzhou, China, an area outside Wuhan.

Methods

Patients admitted to the Infectious Diseases Department of Ruian People’s Hospital in Wenzhou, from January 21 to February 7, 2020, were recruited. Medical data on epidemiological history, demographics, clinical characteristics, laboratory tests, chest computerized tomography (CT) examination, treatment, and short-term outcomes were retrospectively reviewed. Blood biochemistry and routine tests were examined using standard methods and automatic machines. CT examination was performed several times during hospitalization as necessary.

Results

A total of 67 confirmed COVID-19 cases were diagnosed; 64 (95.4%) were common cases and three (4.5%) were severe cases. The most common symptoms at admission were fever (86.6%), cough (77.6%), productive cough (52.2%), chest distress (17.9%), and sore throat (11.9%), followed by diarrhea (7.4%), headache (7.4%), shortness of breath (6.0%), dizziness (4.5%), muscular soreness (4.5%), and running nose (4.5%). Thirty patients (47.8%) had increased C-reactive protein levels. The CT radiographs at admission showed abnormal findings in 54 (80.6%) patients. The patients were treated mainly by oxygen therapy and antiviral drugs. By March 3, 2020, all 67 patients completely recovered and had negative nucleic acid tests. The patients were discharged from the hospital and transferred to a medical observation isolation center for further observation.

Conclusion

Cases of COVID-19 in Wenzhou are milder and have a better prognosis, compared to those in Wuhan. Timely and appropriate screening, diagnosis, and treatment are the key to achieve good outcomes.
Impact of parathyroidectomy on left ventricular function in end stage renal disease patients
Authors: Chen Shaohua , Sheng Kaixiang , Shen Ying , Jiang Hua , Lei Xin , Qu Lihui , Xu Chunping , Chen Jianghua , Zhang Ping
Published: November 2020

Background

Secondary hyperparathyroidism (SHPT) is a common complication in end-stage renal disease (ESRD) patients, and parathyroidectomy (PTX) is an effective treatment intervention of SHPT. However, the curative impact of PTX on left ventricular function still remains incompletely understood. To evaluate the impact of parathyroidectomy on left ventricular function in ESRD patients, we conducted this retrospective study.

Methods

Between Oct 1, 2010 and Oct 1, 2016, ESRD patients presented with SHPT who underwent parathyroidectomy were enrolled. We retrospectively collected the ultrasonic cardiogram parameter pre- and 1-year post-PTX, and analyzed the influence factor for the overturn of left ventricular hypertrophy (LVH) and the improvement of ejection fraction% (EF%).

Results

In all the patients (135), the main ultrasonic cardiogram parameter dramatically improved after PTX. Compared with pre-PTX, the left ventricular mass (LVM) (172.82 (135.90, 212.91) g vs. 192.76 (157.56, 237.97) g, p<0.001) and the left ventricular mass index (LVMI) (107.01 (86.79, 128.42) g/m2 vs. 123.54 (105.49, 146.64) g/m2, p<0.001) significantly declined after 1 year of the PTX. Further, 43.75% patients diagnosed with LVH before the PTX have recovered from LVH. In the subgroup analysis of 35 patients with EF% ≤ 60% pre-PTX, EF% and fractional shortening% (FS%) significantly improved after 1 year of the PTX compared with pre-PTX (EF%: 64.90 ± 7.90% vs. 55.71 ± 4.78%, p<0.001; FS% 35.48 ± 6.34% vs. 29.54 ± 2.88%, p<0.001), and 82.86% patients underwent an improvement of left ventricular systolic function post 1year of the PTX.

Conclusions

tPTX+AT is an effective curative intervention of secondary hyperparathyroidism and can significantly overturn the LVH and increase the left ventricular systolic function.
Insight into the intestinal microbiome of farrowing sows following the administration of garlic (Allium sativum) extract and probiotic bacteria cultures under farming conditions
Authors: Satora Marta , Magdziarz Marcin , Rząsa Anna , Rypuła Krzysztof , Płoneczka-Janeczko Katarzyna
Published: November 2020

Background

Due to the tendency to reduce antibiotic use in humans and animals, more attention is paid to feed additives as their replacement. Crucial role of feed additives is to improve the health status, production efficiency and performance. In this original research, we estimate the potential influence of garlic (Allium sativum) extract and probiotic formula includingEnterococcus faecium,Lactobacillus rhamnosusandLactobacillus fermentumon the intestinal microbiota of sows, using the next generation sequencing method (NGS).

Results

Our results indicate that the overall species richness as well as the composition of swine gut microbiota may be shaped by regular feeding with supplemented additives. On the Family and Genus level both additives (garlic extract and probiotics) seem to decrease microbiome diversity and richness. However, when it comes to garlic supplementation, we found the opposite trend on the Species level.

Conclusions

The analysis of the selected microbial function indicates that both additives used in this study (garlic extract and composition of probiotics) seem to create a greater metabolic potential than estimated in a control group of sows. A general trend of losing or decreasing members of pathogenic species in the swine microbiome seems to occur in relation to both supplemented additives. In the prevention of some bacterial diseases supplemented additives could be considered for future use.
Engineering nature for gaseous hydrocarbon production
Authors: Amer Mohamed , Toogood Helen , Scrutton Nigel S.
Published: November 2020

The development of sustainable routes to the bio-manufacture of gaseous hydrocarbons will contribute widely to future energy needs. Their realisation would contribute towards minimising over-reliance on fossil fuels, improving air quality, reducing carbon footprints and enhancing overall energy security. Alkane gases (propane, butane and isobutane) are efficient and clean-burning fuels. They are established globally within the transportation industry and are used for domestic heating and cooking, non-greenhouse gas refrigerants and as aerosol propellants. As no natural biosynthetic routes to short chain alkanes have been discovered, de novo pathways have been engineered. These pathways incorporate one of two enzymes, either aldehyde deformylating oxygenase or fatty acid photodecarboxylase, to catalyse the final step that leads to gas formation. These new pathways are derived from established routes of fatty acid biosynthesis, reverse β-oxidation for butanol production, valine biosynthesis and amino acid degradation. Single-step production of alkane gases in vivo is also possible, where one recombinant biocatalyst can catalyse gas formation from exogenously supplied short-chain fatty acid precursors. This review explores current progress in bio-alkane gas production, and highlights the potential for implementation of scalable and sustainable commercial bioproduction hubs.
Modification and verification of the Infant–Toddler Meaningful Auditory Integration Scale: a psychometric analysis combining item response theory with classical test theory
Authors: Yang Fengling , Zhao Fei , Zheng Yun , Li Gang
Published: November 2020

Background

Early prelingual auditory development (EPLAD) is a fundamental and important process in the speech and language development of infants and toddlers. The Infant–Toddler Meaningful Auditory Integration Scale (ITMAIS) is a widely used measurement tool for EPLAD, however it has not yet undergone a comprehensive psychometric analysis. The aim of this research was to modify and verify the psychometric properties of ITMAIS using a combination of Item Response Theory (IRT) and Classical Test Theory (CTT).

Methods

Stage 1—1730 children were retrospectively recruited to enable the application of an IRT model, specifically the graded response model, to modify the ITMAIS. Stage 2—another 450 infants and toddlers with normal hearing or permanent hearing loss before auditory intervention were recruited to verify the psychometric properties of the modified ITMAIS (ITMAIS-m) using the CTT method.

Results

Using the metric of the graded response model, by removing item 2 from the ITMAIS, ITMAIS-m demonstrated discrimination parameters ranging from 3.947 to 5.431, difficulty parameters from − 1.146 to 1.150, item information distributed between 4.798 and 9.259 and a test information score of 48.061. None of the items showed differential item functioning. ITMAIS-m was further verified in Stage 2, showing Cronbach’s α of 0.919 and item-total correlations ranging from 0.693 to 0.851. There was good convergent validity of ITMAIS-m with other auditory outcome measure (r = 0.932) and pure tone average thresholds (r ranging from − 0.670 to − 0.909), as well as a high ability to discriminate between different hearing grades (Cohen d ranging from 0.41 to 5.83).

Conclusions

The ITMAIS-m is a reliable and valid tool for evaluating EPLAD in infants and toddlers, which can be efficiently and precisely applied in clinical practice. The combined use of IRT and CTT provides a powerful means to modify psychometrically robust scales aimed at childhood auditory outcome measurements.
Advanced paternal age: effects on sperm parameters, assisted reproduction outcomes and offspring health
Authors: Halvaei Iman , Litzky Julia , Esfandiari Navid
Published: November 2020

Many factors, including postponement of marriage, increased life expectancy, and improved success with assisted reproductive technologies have been contributing to increased paternal age in developed nations. This increased average paternal age has led to concerns about adverse effects of advanced paternal age on sperm quality, assisted reproductive outcomes, and the health of the offspring conceived by older fathers. This review discusses the association between advanced paternal age and sperm parameters, assisted reproduction success rates, and offspring health.