bluuebunny/crossref_metadata_2025 huggingface dataset:
Mindfulness in Sexual Activity, Sexual Satisfaction and Erotic Fantasies in a Non-Clinical Sample
Authors: Sánchez-Sánchez Laura C. , Rodríguez María Fernanda Valderrama , García-Montes José Manuel , Petisco-Rodríguez Cristina , Fernández-García Rubén
Published: January 2021

The goal of this study is to better understand the relation between the practice of Mindfulness and the sexual activity, sexual satisfaction and erotic fantasies of Spanish-speaking participants. This research focuses on the comparison between people who practice Mindfulness versus naïve people, and explores the practice of Mindfulness and its relation with the following variables about sexuality: body awareness and bodily dissociation, personal sexual satisfaction, partner and relationship-related satisfaction, desire, subjective sexual arousal, genital arousal, orgasm, pain, attitudes towards sexual fantasies and types of sexual fantasies. The sample consisted of 106 selected adults, 32 men and 74 women, who completed six measures on an online survey platform: (a) Mindfulness Attention Awareness Scale (MAAS), (b) Scale of Body Connection (SBC), (c) New Sexual Satisfaction Scale (NSSS), (d) Scale of Sexual Activity in Women (SSA-W) and Men (SSA-M), (e) Hurlbert Index of Sexual Fantasy (HISF), (f) Wilson’s Sex Fantasy Questionnaire. In the MAAS, Body Awareness subscale (SBC), NSSS, SSA-W and SSA-M, HISF and intimate fantasies subscale (Wilson’s questionnaire), people in the Mindfulness condition showed higher scores and these differences were statistically significant. These results may have relevant implications in the sexuality of clinical and non-clinical samples.
Determinants of Dribbling and Passing Skills in Competitive Games of Women’s Basketball
Authors: Vencúrik Tomáš , Nykodým Jiří , Bokůvka Dominik , Rupčić Tomislav , Knjaz Damir , Dukarić Vedran , Struhár Ivan
Published: January 2021

This work aimed to identify the influence of selected endogenous (internal load) and exogenous (possession duration, game quarter, and defensive pressure) factors in natural game conditions on the efficiency of dribbling and passing skills. Dribbling and passing skills were assessed during four games of U19 female basketball players and five games of senior (2nd division) female basketball players. In total, 551 dribbling and 914 passing executions were evaluated. Binary logistic regression identified defensive pressure of the opponent as a predictor of dribbling and passing skills effectivity. When the defensive pressure of the opponent was medium, the chances for the ineffective pass were 1.997 times more likely (95% CI; 1.179–3.383), as it is at the minimum defensive pressure. When the defensive pressure of the opponent was high, the chances for ineffective dribbling were 7.45 times more likely (95% CI; 3.672–15.113) and for ineffective pass were 8.419 times more likely (95% CI; 4.6–15.409), as it is at minimum defensive pressure. The game quarter and the internal load were not identified as the predictors influencing the dribbling and passing effectivity. Possession duration was also an insignificant predictor of dribbling and passing skills effectivity. However, the passing skill effectivity decreases when the shot clock is winding down. These findings confirm the importance of transferring game situations into the training process. Coaches should take into account these factors when they want to stimulate determinants of player performance in a balanced and systematic way.
Toward Biofabrication of Resorbable Implants Consisting of a Calcium Phosphate Cement and Fibrin—A Characterization In Vitro and In Vivo
Authors: Ahlfeld Tilman , Lode Anja , Richter Richard Frank , Pradel Winnie , Franke Adrian , Rauner Martina , Stadlinger Bernd , Lauer Günter , Gelinsky Michael , Korn Paula
Published: January 2021

Cleft alveolar bone defects can be treated potentially with tissue engineered bone grafts. Herein, we developed novel biphasic bone constructs consisting of two clinically certified materials, a calcium phosphate cement (CPC) and a fibrin gel that were biofabricated using 3D plotting. The fibrin gel was loaded with mesenchymal stromal cells (MSC) derived from bone marrow. Firstly, the degradation of fibrin as well as the behavior of cells in the biphasic system were evaluated in vitro. Fibrin degraded quickly in presence of MSC. Our results showed that the plotted CPC structure acted slightly stabilizing for the fibrin gel. However, with passing time and fibrin degradation, MSC migrated to the CPC surface. Thus, the fibrin gel could be identified as cell delivery system. A pilot study in vivo was conducted in artificial craniofacial defects in Lewis rats. Ongoing bone formation could be evidenced over 12 weeks but the biphasic constructs were not completely osseous integrated. Nevertheless, our results show that the combination of 3D plotted CPC constructs and fibrin as suitable cell delivery system enables the fabrication of novel regenerative implants for the treatment of alveolar bone defects.
Synergism of AZD6738, an ATR Inhibitor, in Combination with Belotecan, a Camptothecin Analogue, in Chemotherapy-Resistant Ovarian Cancer
Authors: Hur Jin , Ghosh Mithun , Kim Tae Heon , Park Nahee , Pandey Kamal , Cho Young Bin , Hong Sa Deok , Katuwal Nar Bahadur , Kang Minsil , An Hee Jung , Moon Yong Wha
Published: January 2021

Epithelial ovarian cancer remains the leading cause of mortality among all gynecologic malignancies owing to recurrence and ultimate development of chemotherapy resistance in the majority of patients. In the chemotherapy-resistant ovarian cancer preclinical model, we investigated whether AZD6738 (an ataxia telangiectasia and Rad3-related (ATR) inhibitor) could synergize with belotecan (a camptothecin analog and topoisomerase I inhibitor). In vitro, both chemotherapy-resistant and chemotherapy-sensitive ovarian cancer cell lines showed synergistic anti-proliferative activity with a combination treatment of belotecan and AZD6738. The combination also demonstrated synergistic tumor inhibition in mice with a chemotherapy-resistant cell line xenograft. Mechanistically, belotecan, a DNA-damaging agent, increased phospho-ATR (pATR) and phospho-Chk1 (pChk1) in consecutive order, indicating the activation of the DNA repair system. This consequently induced G2/M arrest in the cell cycle analysis. However, when AZD6738 was added to belotecan, pATR and pChk1 induced by belotecan alone were suppressed again. A cell cycle analysis in betotecan showed a sub-G1 increase as well as a G2/M decrease, representing the release of G2/M arrest and the induction of apoptosis. In ascites-derived primary cancer cells from both chemotherapy-sensitive and -resistant ovarian cancer patients, this combination was also synergistic, providing further support for our hypothesis. The combined administration of ATR inhibitor and belotecan proved to be synergistic in our preclinical model. This combination warrants further investigation in a clinical trial, with a particular aim of overcoming chemotherapy resistance in ovarian cancer.
Targeting the WNK-SPAK/OSR1 Pathway and Cation-Chloride Cotransporters for the Therapy of Stroke
Authors: Josiah Sunday Solomon , Meor Azlan Nur Farah , Zhang Jinwei
Published: January 2021

Stroke is one of the major culprits responsible for morbidity and mortality worldwide, and the currently available pharmacological strategies to combat this global disease are scanty. Cation-chloride cotransporters (CCCs) are expressed in several tissues (including neurons) and extensively contribute to the maintenance of numerous physiological functions including chloride homeostasis. Previous studies have implicated two CCCs, the Na+-K+-Cl− and K+-Cl− cotransporters (NKCCs and KCCs) in stroke episodes along with their upstream regulators, the with-no-lysine kinase (WNKs) family and STE20/SPS1-related proline/alanine rich kinase (SPAK) or oxidative stress response kinase (OSR1) via a signaling pathway. As the WNK-SPAK/OSR1 pathway reciprocally regulates NKCC and KCC, a growing body of evidence implicates over-activation and altered expression of NKCC1 in stroke pathology whilst stimulation of KCC3 during and even after a stroke event is neuroprotective. Both inhibition of NKCC1 and activation of KCC3 exert neuroprotection through reduction in intracellular chloride levels and thus could be a novel therapeutic strategy. Hence, this review summarizes the current understanding of functional regulations of the CCCs implicated in stroke with particular focus on NKCC1, KCC3, and WNK-SPAK/OSR1 signaling and discusses the current and potential pharmacological treatments for stroke.
Constitutively Activated DAP12 Induces Functional Anti-Tumor Activation and Maturation of Human Monocyte-Derived DC
Authors: Dalton Robert , Calescibetta Alexandra , Zhou Jun Min , Maurin Michelle , Ward Grace , Trinh Thu Le , Tu Nhan , Gilvary Danielle , Chen Xianghong , Cheng Pingyan , Kostenko Elena , Wei Sheng , Wright Kenneth L. , Eksioglu Erika A.
Published: January 2021

Dendritic cells (DCs) are professional antigen presenting cells with a great capacity for cross-presentation of exogenous antigens from which robust anti-tumor immune responses ensue. However, this function is not always available and requires DCs to first be primed to induce their maturation. In particular, in the field of DC vaccine design, currently available methodologies have been limited in eliciting a sustained anti-tumor immune response. Mechanistically, part of the maturation response is influenced by the presence of stimulatory receptors relying on ITAM-containing activating adaptor molecules like DAP12, that modulates their function. We hypothesize that activating DAP12 in DC could force their maturation and enhance their potential anti-tumor activity for therapeutic intervention. For this purpose, we developed constitutively active DAP12 mutants that can promote activation of monocyte-derived DC. Here we demonstrate its ability to induce the maturation and activation of monocyte-derived DCs which enhances migration, and T cell stimulation in vitro using primary human cells. Moreover, constitutively active DAP12 stimulates a strong immune response in a murine melanoma model leading to a reduction of tumor burden. This provides proof-of-concept for investigating the pre-activation of antigen presenting cells to enhance the effectiveness of anti-tumor immunotherapies.
Mesencephalic Electrical Stimulation Reduces Neuroinflammation after Photothrombotic Stroke in Rats by Targeting the Cholinergic Anti-Inflammatory Pathway
Authors: Schuhmann Michael K. , Papp Lena , Stoll Guido , Blum Robert , Volkmann Jens , Fluri Felix
Published: January 2021

Inflammation is crucial in the pathophysiology of stroke and thus a promising therapeutic target. High-frequency stimulation (HFS) of the mesencephalic locomotor region (MLR) reduces perilesional inflammation after photothrombotic stroke (PTS). However, the underlying mechanism is not completely understood. Since distinct neural and immune cells respond to electrical stimulation by releasing acetylcholine, we hypothesize that HFS might trigger the cholinergic anti-inflammatory pathway via activation of the α7 nicotinic acetylcholine receptor (α7nAchR). To test this hypothesis, rats underwent PTS and implantation of a microelectrode into the MLR. Three hours after intervention, either HFS or sham-stimulation of the MLR was applied for 24 h. IFN-γ, TNF-α, and IL-1α were quantified by cytometric bead array. Choline acetyltransferase (ChAT)+ CD4+-cells and α7nAchR+-cells were quantified visually using immunohistochemistry. Phosphorylation of NFĸB, ERK1/2, Akt, and Stat3 was determined by Western blot analyses. IFN-γ, TNF-α, and IL-1α were decreased in the perilesional area of stimulated rats compared to controls. The number of ChAT+ CD4+-cells increased after MLR-HFS, whereas the amount of α7nAchR+-cells was similar in both groups. Phospho-ERK1/2 was reduced significantly in stimulated rats. The present study suggests that MLR-HFS may trigger anti-inflammatory processes within the perilesional area by modulating the cholinergic system, probably via activation of the α7nAchR.
Nuclear Reorganization in Hippocampal Granule Cell Neurons from a Mouse Model of Down Syndrome: Changes in Chromatin Configuration, Nucleoli and Cajal Bodies
Authors: Puente-Bedia Alba , Berciano María T. , Tapia Olga , Martínez-Cué Carmen , Lafarga Miguel , Rueda Noemí
Published: January 2021

Down syndrome (DS) or trisomy of chromosome 21 (Hsa21) is characterized by impaired hippocampal-dependent learning and memory. These alterations are due to defective neurogenesis and to neuromorphological and functional anomalies of numerous neuronal populations, including hippocampal granular cells (GCs). It has been proposed that the additional gene dose in trisomic cells induces modifications in nuclear compartments and on the chromatin landscape, which could contribute to some DS phenotypes. The Ts65Dn (TS) mouse model of DS carries a triplication of 92 genes orthologous to those found in Hsa21, and shares many phenotypes with DS individuals, including cognitive and neuromorphological alterations. Considering its essential role in hippocampal memory formation, we investigated whether the triplication of this set of Hsa21 orthologous genes in TS mice modifies the nuclear architecture of their GCs. Our results show that the TS mouse presents alterations in the nuclear architecture of its GCs, affecting nuclear compartments involved in transcription and pre-rRNA and pre-mRNA processing. In particular, the GCs of the TS mouse show alterations in the nucleolar fusion pattern and the molecular assembly of Cajal bodies (CBs). Furthermore, hippocampal GCs of TS mice present an epigenetic dysregulation of chromatin that results in an increased heterochromatinization and reduced global transcriptional activity. These nuclear alterations could play an important role in the neuromorphological and/or functional alterations of the hippocampal GCs implicated in the cognitive dysfunction characteristic of TS mice.
The Use of Insecticides to Manage the Western Corn Rootworm, Diabrotica virgifera virgifera, LeConte: History, Field-Evolved Resistance, and Associated Mechanisms
Authors: Meinke Lance J. , Souza Dariane , Siegfried Blair D.
Published: January 2021

The western corn rootworm, Diabrotica virgifera virgifera LeConte (Dvv) is a significant insect pest of maize in the United States (U.S.). This paper reviews the history of insecticide use in Dvv management programs, Dvv adaptation to insecticides, i.e., field-evolved resistance and associated mechanisms of resistance, plus the current role of insecticides in the transgenic era. In the western U.S. Corn Belt where continuous maize is commonly grown in large irrigated monocultures, broadcast-applied soil or foliar insecticides have been extensively used over time to manage annual densities of Dvv and other secondary insect pests. This has contributed to the sequential occurrence of Dvv resistance evolution to cyclodiene, organophosphate, carbamate, and pyrethroid insecticides since the 1950s. Mechanisms of resistance are complex, but both oxidative and hydrolytic metabolism contribute to organophosphate, carbamate, and pyrethroid resistance facilitating cross-resistance between insecticide classes. History shows that Dvv insecticide resistance can evolve quickly and may persist in field populations even in the absence of selection. This suggests minimal fitness costs associated with Dvv resistance. In the transgenic era, insecticides function primarily as complementary tools with other Dvv management tactics to manage annual Dvv densities/crop injury and resistance over time.
PCE3 Plays a Role in the Reproduction of Male Nilaparvata lugens
Authors: Zheng Rong-er , Ji Jinliang , Wu Jiamin , Zhang Ruijuan , Li Yabin , Yu Xiaoping , Xu Yipeng
Published: January 2021

Nilaparvata lugens proclotting enzymes (NlPCEs) belong to the clip domain serine protease (clip-SP) family, which is a characteristic protease family in arthropods. NlPCE3 was previously reported to regulate egg production and development in female N. lugens, but its role in male N. lugens is unclear. In the present study, qPCR analysis showed that NlPCE3 was expressed in three different tissues (gut, testis and fat body). RNAi revealed that dsNlPCE3 injection made the male vas deferens thinner and reduced the oviposition level of the females that mated with dsNlPCE3-treated males, causing eggs not to hatch. Furthermore, immunofluorescence staining showed that NlPCE3 was widely expressed in the male internal genitalia. However, after dsNlPCE3 injection, expression of NlPCE3 was diffuse in the male internal genitalia, whose peripheral cells seemed degraded. Overall, these results indicate that NlPCE3 is important for reproduction in male N. lugens.
Cutaneous and Mucosal Melanomas of Uncommon Sites: Where Do We Stand Now?
Authors: Dika Emi , Lambertini Martina , Pellegrini Cristina , Veronesi Giulia , Melotti Barbara , Riefolo Mattia , Sperandi Francesca , Patrizi Annalisa , Ricci Costantino , Mussi Martina , Fargnoli Maria Concetta
Published: January 2021

Melanomas arising at uncommon sites include a group of lesions related to unusual localizations in specific ethnic groups. The rarity of the disease often represents a limit to the participation of patients in specific trials. However, this peculiar genetic scenario has important therapeutic implications regarding new oncologic therapies. The aim of this article is to review the clinical features, somatic alterations and therapeutic options for melanomas of uncommon sites. They can be classified as cutaneous and mucosal lesions affecting the nail apparatus, palms/soles, oral mucosa, genital area and scalp. The prognosis may be worse compared to melanomas of other districts, and a prompt diagnosis may dramatically influence the outcome. Dermatologists and oncologists should therefore distinguish this melanoma subgroup in terms of surgical intervention and medical treatment. Due to the lack of mutations in genes usually found in cutaneous melanomas, the discovery of novel targets is required to develop new strategies and to change the prognosis of non-responders or wild-type patients.
Current Knowledge and Future Challenges in Takotsubo Syndrome: Part 1—Pathophysiology and Diagnosis
Authors: Rawish Elias , Stiermaier Thomas , Santoro Francesco , Brunetti Natale , Eitel Ingo
Published: January 2021

First recognized in 1990, takotsubo syndrome (TTS) constitutes an acute cardiac condition that mimics acute myocardial infarction commonly in the absence of obstructive coronary artery disease; it is characterized by temporary left ventricular dysfunction, regularly in a circumferential apical, midventricular, or basal distribution. Considering its acute clinical presentation, coronary angiography with left ventriculography constitutes the gold standard diagnostic tool to exclude or confirm TTS. Frequently, TTS is related to severe emotional or physical stress and a subsequent increased adrenergic stimulation affecting cardiac function. Beyond clinical presentation, epidemiology, and novel diagnostic biomarkers, this review draws attention to potential pathophysiological mechanisms for the observed reversible myocardial dysfunction such as sympathetic overdrive-mediated multi-vessel epicardial spasms, microvascular dysfunction, the direct toxicity of catecholamines, lipotoxicity, and inflammation. Considering the long-term prognosis, further experimental and clinical research is indispensable to elucidate further pathophysiological mechanisms underlying TTS before randomized control trials with evidence-based therapeutic management can be performed.
Effects of β-Lactolin on Regional Cerebral Blood Flow within the Dorsolateral Prefrontal Cortex during Working Memory Task in Healthy Adults: A Randomized Controlled Trial
Authors: Ano Yasuhisa , Kita Masahiro , Kobayashi Keiko , Koikeda Takashi , Kawashima Ryuta
Published: January 2021

Epidemiological studies have reported that consumption of dairy products rich in β-lactolin is beneficial for cognitive decline among elderly individuals. Although previous studies have shown that β-lactolin supplementation improves memory function and attention in healthy adults, the mechanism through which β-lactolin affects human brain function has yet to be elucidated. This placebo-controlled randomized double-blind study therefore examined the effects of β-lactolin on human regional cerebral blood flow (rCBF) using near-infrared spectroscopy (NIRS) according to the Consolidated Standards of Reporting Trials guidelines. A total of 114 healthy participants aged between 50 and 75 years with relatively low cognition were randomly allocated into the β-lactolin or placebo groups (n = 57 for both groups) and received supplementation for 6 weeks. After the 6 weeks of supplementation, total hemoglobin during cognitive tasks (Kraepelin and 2-back tasks) was measured using two-channel NIRS to determine rCBF. Accordingly, the β-lactolin group had significantly higher changes in total hemoglobin at the left dorsolateral prefrontal cortex (DLPFC) area measured using the left-side channel during the 2-back tasks (p = 0.027) compared to the placebo group. The present study suggests that β-lactolin supplementation increases rCBF and DLPFC activity during working memory tasks.
TRPM5 rs886277 Polymorphism Predicts Hepatic Fibrosis Progression in Non-Cirrhotic HCV-Infected Patients
Authors: Resino Salvador , Fernández-Rodríguez Amanda , Pineda-Tenor Daniel , Gómez-Moreno Ana Zaida , Sánchez-Ruano Juan José , Artaza-Varasa Tomas , Muñoz-Gómez María José , Virseda-Berdices Ana , Martín-Vicente María , Martínez Isidoro , Jiménez-Sousa María A.
Published: January 2021

Background: TRPM5 (transient receptor potential cation channel subfamily M member 5) rs886277 polymorphism has been related to liver cirrhosis from different etiologies. The present study investigates the association of TRPM5 rs886277 polymorphism with liver fibrosis progression and cirrhosis development in chronic hepatitis C (CHC) patients. Methods: We conducted a retrospective study of 208 non-cirrhotic patients with CHC, who had at least two liver stiffness measurements (LSM) with a separation of 12 months (baseline LSM (LSM1) and the last LSM (LSM2)). Two outcome variables were considered: (1) LSM2/LSM1 ratio; (2) cirrhosis progression (F4; LSM ≥ 12.5 kPa). DNA genotyping was done at the CeGen using a MassARRAY platform. Results: The follow-up time was similar irrespective of the rs886277 genotype (46.4 months in TT genotype, 46.4 months in CT genotype, and 49.2 months in CC genotype; p = 0.649). The highest LSM increases were found in patients with CC genotype compared with TT and CT genotypes (p = 0.044 and p = 0.038, respectively). The cirrhosis progression was higher in patients with CC genotype than TT genotype (p = 0.033). Thus, the rs886277 C allele was associated with higher cirrhosis progression (adjusted odds ratio (aOR) = 2.64; p = 0.014). Moreover, rs886277 CC genotype was also related to higher values of LSM2/LSM1 ratio (adjusted arithmetic mean ratio a(AMR) = 1.31; p = 0.001) and cirrhosis progression (aOR = 4.33; p = 0.027). Conclusions: TRPM5 rs886277 polymorphism was associated with liver fibrosis progression and cirrhosis development among hepatitis C virus (HCV)-infected patients. Specifically, the rs886277 C allele and CC genotype were risk factors for advancing liver fibrosis and cirrhosis compared to the rs886277 T allele and CT/TT genotype, respectively.
Subthreshold Nano-Second Laser Treatment and Age-Related Macular Degeneration
Authors: Cohn Amy C. , Wu Zhichao , Jobling Andrew I. , Fletcher Erica L. , Guymer Robyn H.
Published: January 2021

The presence of drusen is an important hallmark of age-related macular degeneration (AMD). Laser-induced regression of drusen, first observed over four decades ago, has led to much interest in the potential role of lasers in slowing the progression of the disease. In this article, we summarise the key insights from pre-clinical studies into the possible mechanisms of action of various laser interventions that result in beneficial changes in the retinal pigment epithelium/Bruch’s membrane/choriocapillaris interface. Key learnings from clinical trials of laser treatment in AMD are also summarised, concentrating on the evolution of laser technology towards short pulse, non-thermal delivery such as the nanosecond laser. The evolution in our understanding of AMD, through advances in multimodal imaging and functional testing, as well as ongoing investigation of key pathological mechanisms, have all helped to set the scene for further well-conducted randomised trials to further explore potential utility of the nanosecond and other subthreshold short pulse lasers in AMD.
A New Hybrid Inversion Method for 2D Nuclear Magnetic Resonance Combining TSVD and Tikhonov Regularization
Authors: Landi Germana , Zama Fabiana , Bortolotti Villiam
Published: January 2021

This paper is concerned with the reconstruction of relaxation time distributions in Nuclear Magnetic Resonance (NMR) relaxometry. This is a large-scale and ill-posed inverse problem with many potential applications in biology, medicine, chemistry, and other disciplines. However, the large amount of data and the consequently long inversion times, together with the high sensitivity of the solution to the value of the regularization parameter, still represent a major issue in the applicability of the NMR relaxometry. We present a method for two-dimensional data inversion (2DNMR) which combines Truncated Singular Value Decomposition and Tikhonov regularization in order to accelerate the inversion time and to reduce the sensitivity to the value of the regularization parameter. The Discrete Picard condition is used to jointly select the SVD truncation and Tikhonov regularization parameters. We evaluate the performance of the proposed method on both simulated and real NMR measurements.
The Role of Environmental Factors on the Fishery Catch of the Squid Uroteuthis chinensis in the Pearl River Estuary, China
Authors: Wang Dongliang , Yao Lijun , Yu Jing , Chen Pimao
Published: January 2021

The Pearl River Estuary (PRE) is one of the major fishing grounds for the squid Uroteuthis chinensis. Taking that into consideration, this study analyzes the environmental effects on the spatiotemporal variability of U. chinensis in the PRE, on the basis of the Generalized Additive Model (GAM) and Clustering Fishing Tactics (CFT), using satellite and in situ observations. Results show that 63.1% of the total variation in U. chinensis Catch Per Unit Effort (CPUE) in the PRE could be explained by looking into outside factors. The most important one was the interaction of sea surface temperature (SST) and month, with a contribution of 26.7%, followed by the interaction effect of depth and month, fishermen’s fishing tactics, sea surface salinity (SSS), chlorophyll a concentration (Chl a), and year, with contributions of 12.8%, 8.5%, 7.7%, 4.0%, and 3.1%, respectively. In summary, U. chinensis in the PRE was mainly distributed over areas with an SST of 22–29 °C, SSS of 32.5–34‰, Chl a of 0–0.3 mg × m−3, and water depth of 40–140 m. The distribution of U. chinensis in the PRE was affected by the western Guangdong coastal current, distribution of marine primary productivity, and variation of habitat conditions. Lower stock of U. chinensis in the PRE was connected with La Niña in 2008.
Plasma Extracellular Vesicle Characteristics Correlate with Tumor Differentiation and Predict Overall Survival in Patients with Pancreatic Ductal Adenocarcinoma Undergoing Surgery with Curative Intent
Authors: Badovinac David , Goričar Katja , Zavrtanik Hana , Petrič Miha , Lavrin Teja , Mavec Nina , Dolžan Vita , Tomažič Aleš , Lenassi Metka
Published: January 2021

Better preoperative characterization of patients with pancreatic ductal adenocarcinoma (PDAC) would aid in treatment optimization. Extracellular vesicles (EV) are promising, largely unexplored biomarkers in PDAC. This study aimed to evaluate if plasma EV characteristics are associated with PDAC clinical characteristics and overall survival (OS). The prospective cohort included 34 PDAC patients undergoing surgery with curative intent. Patient data and plasma samples were collected preoperatively, intraoperatively and one month postoperatively. Small plasma EV (sEV) concentration and size were determined by nanoparticle-tracking analysis. A Mann–Whitney test, Spearman’s rho and Cox regression were used in statistical analysis. Preoperatively, patients with poorly differentiated tumors had significantly larger plasma sEVs when compared to patients with well/moderately differentiated tumors (mean diameter 176.9 vs. 149.2 nm, p = 0.021), the sEV size even enabling discrimination of the two groups (AUC = 0.742, 95% CI = 0.560–0.923). Plasma sEV characteristics were also a predictor of OS in multivariable analysis. Patients with a more than 33.8% increase in sEV concentration after one month had 7.2 months shorter median OS (p = 0.002), while patients with a more than 28.0% decrease in sEV size had 9.2 months shorter median OS (p = 0.045). Plasma sEV concentration and size correlate with tumor differentiation and may predict OS in PDAC patients. In the future, plasma sEV characteristics could contribute to improved patient stratification for optimized treatment.
Predictors of Successful Reading Comprehension in Bilingual Adults: The Role of Reading Strategies and Language Proficiency
Authors: Friesen Deanna C. , Frid Bailey
Published: January 2021

The current study investigated the type of strategies that English–French bilingual adults utilize when reading in their dominant and non-dominant languages and which of these strategies are associated with reading comprehension success. Thirty-nine participants read short texts while reporting aloud what they were thinking as they read. Following each passage, readers answered three comprehension questions. Questions either required information found directly in the text (literal question) or required a necessary inference or an elaborative inference. Readers reported more necessary and elaborative inferences and referred to more background knowledge in their dominant language than in their non-dominant language. Engaging in both text analysis strategies and meaning extraction strategies predicted reading comprehension success in both languages, with differences observed depending on the type of question posed. Results are discussed with respect to how strategy use supports the development of text representations.
Additive Linking in L2 French Discourse by German Learners: Syntactic Embedding and Intonation Patterns
Authors: Sandra Benazzo , Christine Dimroth , Santiago Fabian
Published: January 2021

This study deals with the expression of additive linking in L2 French by adult German learners with two proficiency levels (advanced vs. intermediate). We examine whether crosslinguistic influences are observed in three domains: the frequency and type of additive expressions in discourse, the syntactic integration of additive particles in the utterance and the prosodic contour associated with them. A total of 70 participants (20 French native speakers, 20 German native speakers and 30 German learners of L2 French) performed an oral narrative task elicited via a video clip presenting abundant additive contexts. Our results show that advanced German learners did not experience an L1 transfer in any of the domains analyzed, but instead they show a learner-specific tendency to overmark the contrastive status of the relevant entities in discourse. Yet traces of crosslinguistic influence are visible in intermediate learners’ choice and frequency of additive means, as well as the preferred position of the particles. All learners seem to have quickly discarded the possibility to mark scope by prosody, in contrast to what they do in their L1. We discuss these findings in the light of the L2 acquisition of cohesive devices in discourse and their interactions with different linguistic levels.
Acknowledgment to Reviewers of Laws in 2020
Authors:   Laws Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Laws maintains its standards for the high quality of its published papers [...]
The Alteration of Intestinal Microbiota Profile and Immune Response in Epinephelus coioides during Pathogen Infection
Authors: Xiao Joe Joan Tang , Tseng Yung-Che , Wu Jen-Leih , Lu Ming-Wei
Published: January 2021

Epinephelus coioides, or grouper, is a high economic value fish species that plays an important role in the aquaculture industry in Asia. However, both viral and bacterial diseases have threatened grouper for many years, especially nervous necrosis virus, grouper iridovirus and Vibrio harveyi, which have caused a bottleneck in the grouper industry. Currently, intestinal microbiota can provide novel insights into the pathogenesis-related factors involved in pathogen infection. Hence, we investigated the comparison of intestinal microbiota communities in control group and pathogen-infected grouper through high-throughput sequencing of the 16S rRNA gene. Our results showed that microbial diversity was decreased, whereas microbial richness was increased during pathogen infection. The individuals in each group were distributed distinctly on the PLSDA diagram, especially the GIV group. Proteobacteria and Firmicutes were the most abundant bacterial phyla in all groups. Interestingly, beneficial genera, Faecalibacterium and Bifidobacterium, predominated in the intestines of the control group. In contrast, the intestines of pathogen-infected grouper had higher levels of harmful genera such as Sphingomonas, Atopostipes, Staphylococcus and Acinetobacter. Additionally, we investigated the expression levels of innate and adaptive immune-related genes after viral and bacterial infection. The results revealed that immunoglobulin T and proinflammatory cytokine levels in the intestine increased after pathogen infection. Through these unique bacterial compositions in diseased and uninfected fish, we could establish a novel therapeutic approach and bacterial marker for preventing and controlling these diseases.
Solubility of Carbon Dioxide in Deep Eutectic Solvents Based on 3-Amino-1-Propanol and Tetraalkylammonium Salts at Low Pressure
Authors: Cichowska-Kopczyńska Iwona , Warmińska Dorota , Nowosielski Bartosz
Published: January 2021

Deep eutectic solvents (DESs) became an object of a great interest as an alternative to ionic liquids (ILs) and commonly used in CO2 capture amine solutions. In the present study, five different DESs based on 3-amino-1-propanol as physical-chemical CO2 absorbents were used. The composition was chosen in order to estimate the effects of hydrogen bond acceptor:hydrogen bond donor (HBA:HBD) molar ratio, anion type and length of alkyl chain of composing salt. The Fourier Transform Infrared (FTIR) spectroscopy was used to confirm chemical reaction. The solubility of CO2 was measured at low pressures up to 170 kPa at the temperature range of 293–318 K. Viscosity, polarity and Kamlet–Taft parameters were determined in order to estimate the dependences of the parameters and the CO2 capacity. CO2 uptake was observed to improve with decreasing molar ratio of hydrogen bond donor. Comparing the CO2 capacity of [TBAC]-based DESs, at the approximate pressure of 50 kPa, it was observed that the capacity increased in the following order of molar ratios—1:8 < 1:6 < 1:4 and a decrease in molar ratio from 1:8 to 1:4 resulted in about a 100% increase of capacity. Compared to [TBAC][AP] DESs, the [TEAC][AP] 1:4 and [TBAB][AP] 1:4 exhibited higher CO2 uptake, though the best results were obtained for [TBAB][AP].
Nanostructured Lipid Carriers Engineered as Topical Delivery of Etodolac: Optimization and Cytotoxicity Studies
Authors: Czajkowska-Kośnik Anna , Szymańska Emilia , Czarnomysy Robert , Jacyna Julia , Markuszewski Michał , Basa Anna , Winnicka Katarzyna
Published: January 2021

Etodolac (ETD), a nonsteroidal anti-inflammatory drug, exhibits antinflammatory, analgesic, and antipyretic activity. The main type of ETD administration is oral route, which is associated with significant systemic side effects. Nanostructured lipid carriers (NLC), a modern lipid formulation, are non-toxic, biocompatible, can improve the solubility and stability of drugs. Nanostructured lipid carriers (NLC) containing etodolac were prepared by a melt-emulsification and ultrasonication technique. Full factorial design (FFD) was applied to optimize the composition of NLC and their properties such as zeta potential, polidyspersity index, and entrapment efficiency. Formulations consisting of Capryol 90, glicerol monostearate, and Tween 20 displayed particle size below 300 nm, encapsulated drug with efficiency of approximately 87% and prolonged drug release up to 24 h. Stable formulations displayed moderately negative surface charge suggesting their limited ability to interact with skin surface but simultaneously presenting their lower risk to cause cell-membrane disruption. In fact, cytotoxicity assessment using human dermal fibroblasts and human epidermal keratinocytes revealed that etodolac-loaded NLC had no important impact on skin cells viability evaluated in vitro, which might evidence that NLC formulations are safe for dermal delivery. The studies developed were relatively fast and simple, requiring no specialized equipment method to prepare NLC as ETD carriers ensuring better solubility and prolonged drug release.
The Numerical Validation of the Adomian Decomposition Method for Solving Volterra Integral Equation with Discontinuous Kernels Using the CESTAC Method
Authors: Noeiaghdam Samad , Sidorov Denis , Wazwaz Abdul-Majid , Sidorov Nikolai , Sizikov Valery
Published: January 2021

The aim of this paper is to present a new method and the tool to validate the numerical results of the Volterra integral equation with discontinuous kernels in linear and non-linear forms obtained from the Adomian decomposition method. Because of disadvantages of the traditional absolute error to show the accuracy of the mathematical methods which is based on the floating point arithmetic, we apply the stochastic arithmetic and new condition to study the efficiency of the method which is based on two successive approximations. Thus the CESTAC method (Controle et Estimation Stochastique des Arrondis de Calculs) and the CADNA (Control of Accuracy and Debugging for Numerical Applications) library are employed. Finding the optimal iteration of the method, optimal approximation and the optimal error are some of advantages of the stochastic arithmetic, the CESTAC method and the CADNA library in comparison with the floating point arithmetic and usual packages. The theorems are proved to show the convergence analysis of the Adomian decomposition method for solving the mentioned problem. Also, the main theorem of the CESTAC method is presented which shows the equality between the number of common significant digits between exact and approximate solutions and two successive approximations.This makes in possible to apply the new termination criterion instead of absolute error. Several examples in both linear and nonlinear cases are solved and the numerical results for the stochastic arithmetic and the floating-point arithmetic are compared to demonstrate the accuracy of the novel method.
A Fuzzy Evaluation Decision Model for the Ratio Operating Performance Index
Authors: Li Mingyuan , Chen Kuen-Suan , Yu Chun-Min , Yang Chun-Ming
Published: January 2021

In order to take into account cost and timeliness and enhance accuracy testing, this study developed the fuzzy number and membership function, using the confidence interval of ratio operating performance index. Subsequently, according to the statistical test rules and the application of the fuzzy number and membership function, a fuzzy evaluation decision model for the operating performance index is proposed, to evaluate if the business performance reaches the needed level. Based on the abovementioned, the evaluation model in this study took into account not only timeliness but also accuracy, so that it could grasp the opportunity of improvement for operating organizations with poor operating performance after being evaluated. This fuzzy evaluation decision model for the operating performance index constructs a fuzzy membership function retrieved from an index’s confidence interval, reducing the chance of miscalculation due to sampling mistakes and improving the efficiency of evaluation. Finally, in order to facilitate the application of readers and the industry, this paper uses cases to explain the proposed fuzzy verification method. On the whole, the model proposed in this paper is a data-based auxiliary tool for the service operating performance improvement strategy.
Cinnamaldehyde Could Reduce the Accumulation of Diarrhetic Shellfish Toxins in the Digestive Gland of the Mussel Perna viridis under Laboratory Conditions
Authors: Duan Guo-Fang , Liu Yang , Zhang Li-Na , Li Hong-Ye , Liu Jie-Sheng , Yang Wei-Dong
Published: January 2021

Diarrhetic shellfish toxins (DSTs), some of the most important phycotoxins, are distributed almost all over the world, posing a great threat to human health through the food chain. Therefore, it is of great significance to find effective methods to reduce toxin accumulation in shellfish. In this paper, we observed the effects of four phytochemicals including cinnamaldehyde (CA), quercetin, oridonin and allicin on the accumulation of DSTs in the digestive gland of Perna viridis after exposure to the DSTs-producing Prorocentrum lima. We found that, among the four phytochemicals, CA could effectively decrease the accumulation of DSTs (okadaic acid-eq) in the digestive gland of P. viridis. Further evidence demonstrated that CA could reduce the histological alterations of the digestive gland of a mussel caused by DSTs. RT-qPCR showed that CA could suppress the CYP3A4 induction by DSTs, suggesting that the DSTs’ decrease induced by CA might be related to the inhibition of CYP3A4 transcription induction. However, further studies on the underlying mechanism, optimal treatment time, ecological safety and cost should be addressed before cinnamaldehyde is used to decrease the accumulation of DSTs in field.
Course of Metal Ions after a Revision of Malfunctioning Metal-on-Metal Total Hip Prostheses
Authors: Nicolli Annamaria , Bortoletti Isabella , Maso Stefano , Trevisan Andrea
Published: January 2021

The present research evaluated the course of cobalt and chromium in the blood and urine after the revision of metal-on-metal with a ceramic-on-polyethylene total hip arthroplasty. Seven patients were enrolled for hip prosthesis revision owing to ascertained damage of the implant. Metals in the blood and urine were evaluated before and after the hip revision. The double measurement before the total hip revision revealed high levels of metal ions (on average, 88.1 µg/L of cobalt in the blood, 399.0 µg/g of creatinine cobalt in the urine, 46.8 µg/L of chromium in the blood, and 129.6 µg/g of creatinine chromium in the urine at the first measurements), with an increasing trend between the first and second dosage. Within a week after the hip revision, the levels of metal ions significantly decreased by approximately half. Four to six months after the operation, the cobalt levels were found near to the reference values, whereas the chromium levels reached 25% of the values measured before the revision. The revision of malfunctioning metal-on-metal implants produced a dramatic decrease of metal ions in biological fluids, although it did not completely rescue the chromium level.
Ballistic Impact Resistance of Bulletproof Vest Inserts Containing Printed Titanium Structures
Authors: Zochowski Pawel , Bajkowski Marcin , Grygoruk Roman , Magier Mariusz , Burian Wojciech , Pyka Dariusz , Bocian Miroslaw , Jamroziak Krzysztof
Published: January 2021

Finite element modeling of ballistic impact of inserts containing titanium structures were presented in the article. The inserts containing an additional layer made using additive manufacturing technology were analyzed. The layer was created from repetitive elements made without connections (adjacent cells were inseparable). Four variants of printed titanium structures were placed between layers of Twaron CT 750 aramid fabric to create ballistic inserts. In order to assess the ballistic resistance of the inserts, numerical simulations of ballistic impact phenomenon were carried out using LS-Dyna software. In the simulations the inserts were placed on a steel box filled with ballistic clay and were fired at with the 9 × 19 mm full metal jacket (FMJ) Parabellum projectile. The main aim of the work was to check the effectiveness of such solutions in soft ballistic protection applications and to select the most effective variant of 3D printed structure. Results of the numerical analysis showed a high potential for 3D printed structures made of titanium alloys to be used for bulletproof vest inserts. In all analyzed cases the projectile was stopped by the armor. In addition, thanks to the cooperation of adjacent cells, the projectile energy density was distributed over a large area, as evidenced by large volumes of hollows in the ballistic clay. The indentations in the ballistic clay obtained in the simulations were significantly lower than the acceptable value for the back face deformation (BFD) parameter required by international body armor standards.
Characterization of the Field Fludioxonil Resistance and Its Molecular Basis in Botrytis cinerea from Shanghai Province in China
Authors: Wang Weizhen , Fang Yuan , Imran Muhammad , Hu Zhihong , Zhang Sicong , Huang Zhongqiao , Liu Xili
Published: January 2021

Botrytis cinerea is a destructive necrotrophic pathogen that can infect many plant species. The control of gray mold mainly relies on the application of fungicides, and the fungicide fludioxonil is widely used in China. However, the field fungicide resistance of B. cinerea to this compound is largely unknown. In this study, B. cinerea isolates were collected from different districts of Shanghai province in 2015–2017, and their sensitivity to fludioxonil was determined. A total of 65 out of 187 field isolates (34.76%) were found to be resistant to fludioxonil, with 36 (19.25%) showing high resistance and 29 (15.51%) showing moderate resistance. Most of these resistant isolates also showed resistance to iprodione, and some developed resistance to fungicides of other modes of action. AtrB gene expression, an indicator of MDR1 and MDR1h phenotypes, was not dramatically increased in the tested resistant isolates. Biological characteristics and osmotic sensitivity investigations showed that the fitness of resistant isolates was lower than that of sensitive ones. To investigate the molecular resistance mechanisms of B. cinerea to fludioxonil, the Bos1 amino acid sequences were compared between resistant and sensitive isolates. Resistant isolates revealed either no amino acid variations or the mutations I365S, I365N, Q369P/N373S, and N373S.