bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamics of Antibiotic Resistance of Streptococcus pneumoniae in France: A Pediatric Prospective Nasopharyngeal Carriage Study from 2001 to 2022
Authors: Rybak Alexis , Levy Corinne , Ouldali Naïm , Bonacorsi Stéphane , Béchet Stéphane , Delobbe Jean-François , Batard Christophe , Donikian Isabelle , Goldrey Marie , Assouline Jessica , Cohen Robert , Varon Emmanuelle
Published: June 2023

Epidemiological surveillance of nasopharyngeal pneumococcal carriage is important for monitoring serotype distribution and antibiotic resistance, particularly before and after the implementation of pneumococcal conjugate vaccines (PCVs). With a prospective surveillance study in France, we aimed to analyze the dynamics of pneumococcal carriage, antibiotic susceptibility and serotype distribution in children aged 6 to 24 months who had acute otitis media between 2001 and 2022 with a focus on the late PCV13 period from May 2014 to July 2022. Trends were analyzed with segmented linear regression with autoregressive error. For the 17,136 children enrolled, overall pneumococcal carriage was stable during the study. During the late PCV13 period, the five most frequent serotypes were all non-PCV13 serotypes: 15B/C (14.3%), 23B (11.0%), 11A (9.6%), 15A (7.4%) and 35B (6.5%). During the same period, we observed a rebound of penicillin non-susceptibility (+0.15% per month, 95% confidence interval, +0.08 to 0.22, p < 0.001). Five serotypes accounted for 64.4% of the penicillin non-susceptible strains: 11A (17.5%), 35B (14.9%), 15A (13.9%), 15B/C (9.9%) and 19F (8.2%); non-PCV13/PCV15 accounted for <1%, and non-PCV15/PCV20 accounted for 28%. The next generation PCVs, particularly PCV20, may disrupt nasopharyngeal carriage and contribute to decreasing the rate of antibiotic resistance among pneumococci.
Comment on Villalva et al. Antioxidant, Anti-Inflammatory, and Antibacterial Properties of an Achillea millefolium L. Extract and Its Fractions Obtained by Supercritical Anti-Solvent Fractionation against Helicobacter pylori. Antioxidants 2022, 11, 1849
Authors: Frański Rafał , Beszterda-Buszczak Monika
Published: June 2023

Villalva et al. evaluated the potential utility of an Achillea millefolium (yarrow) extract in the control of H. pylori infection. The agar-well diffusions bioassay was applied to determine the antimicrobial activity of yarrow extracts. The supercritical anti-solvent fractionation process of yarrow extract was made to give two different fractions with polar phenolic compounds and monoterpenes and sesquiterpenes, respectively. Phenolic compounds were identified by HPLC-ESIMS by using the accurate masses of [M−H]− ions and the characteristic product ions. However, some of the reported product ions seem to be disputable, as described below.
Modeling of Walking-Gait Parameters and Walking Strategy for Quadruped Robots
Authors: Li Zhaolu , Song Yumin , Zhang Xiaoli , Peng Xiafu , Xu Ning
Published: June 2023

The inspiration for the footed robot was originally derived from biology, and it was an imitation of biological form and movement. In this paper, a bionic-robot dog is designed to reveal the motion characteristics of a quadruped robot mechanism through modeling, model kinematic analysis, and other methods. First, the structural characteristics and movement characteristics of the developed bionic-dog model are studied. The first step is to study the physiological structure of the dog, analyze the function of the dog’s limbs, and then use a high-speed camera to capture the motion of the marked bionic-robot dog and shoot motion video of the bionic-robot dog in different motion states. The effective data of the marked points in the video are extracted using PHOTRON 1.0 software, and the extracted data are analyzed and processed in the software MATLAB R2020a, and finally the structural characteristics and motion laws of the bionic-robot dog are obtained. Then, a bionic-robot-dog experimental platform is built to conduct experiments with three planned gaits (dynamic gait, static gait, and gait transition). The experiments showed that the three gaits were consistent with the planned movements and the bionic-robot dog could perform stable fast-gait walking, slow-gait walking, and quickly complete gait transitions. All three gaits were simulated in ADAMS 2019 software, and the simulation results showed that all three gaits caused the bionic dog robot to move smoothly.
Recent Progress in Synthesis and Photonic Applications of Two-Dimensional Bismuthene
Authors: Li Haoran , Yang Zhibin
Published: June 2023

The emergence of phosphorene has generated significant interest in 2D group VA nanomaterials. Among this group, bismuthene exhibits layer-dependent direct bandgaps, high carrier mobility, and topological insulator properties because of its unique structure and ultrathin nature, distinguishing it as a promising candidate for photonic applications. Particularly, its outstanding stability in air makes bismuthene more advantageous than phosphorene for practical applications. Here, we provide a comprehensive review of recent advances regarding 2D bismuth by focusing on the aspects of methods of synthesis and photonic applications. First, the structure and fundamental properties of bismuthene are described, referring to its crystallinity and band structures, as well as to its nonlinear optical properties. Subsequently, the common synthesis methods for 2D bismuth are summarized, including both top-down and bottom-up approaches. Then, potential photonic applications based on 2D bismuth, involving nonlinear photonic devices, photocatalyst, and photodetectors, are illustrated. The performance, mechanisms, and features of the devices are discussed. Finally, the review is summarized and some challenges and future outlooks in this field are addressed.
Running Speed Loss Can Be Prevented with Passive Heat Maintenance before the Start of the Soccer Match
Authors: Majkić Branislav , Stojanović Nikola , Marković Andrea , Stojiljković Nenad , Olanescu Mihai , Suciu Adrian , Peris Miruna , Plesa Alin
Published: June 2023

Maintaining the state achieved after the warm-up in modern soccer represents a significant problem. The main goal of this research was to determine the influence of a regular tracksuit on skin temperature and running speed during the pre-game routine. This study included 36 youth soccer players (15.61 ± 0.68 years, 175.16 ± 4.21 cm) divided into two groups. A two-way ANOVA was used with the level of significance set at <0.05. The main findings of this research indicated that skin temperature is reduced after the WU and is slower to be restored when players wear tracksuits. In addition, the results showed a significant treatment × time-point interaction effect for 10 [p = 0.015, ηp2 = 0.16], 20 [p = 0.001, ηp2 = 0.26], and 30 [p = 0.005, ηp2 = 0.20] meter sprint tests. A significant treatment (experimental vs. control) main effect was observed for 10 m [p = 0.042, ηp2 = 0.35], 20 m [p = 0.020, ηp2 = 0.55], and 30 m [p = 0.005, ηp2 = 0.58] sprint tests. Moreover, a significant sequence-trial interaction effect for 10 m (p = 0.002), 20 m (p < 0.001), and 30 m (p < 0.001) sprints was observed. However, the main effects of the sequence or trial factors alone were insignificant. It is concluded that regular tracksuits may have a beneficial influence on Ts loss and running speed. This research’s most significant practical application is that it lasts for a short period of time and that it requires no extra effort.
Prediction of Near-Wake Velocity in Laminar Flow over a Circular Cylinder Using Neural Networks with Instantaneous Wall Pressure Input
Authors: Yun Jinhyeok , Lee Jungil
Published: June 2023

In the present study, to predict the transverse velocity field in the near-wake of laminar flow over a circular cylinder at the Reynolds numbers of 60 and 300, we construct neural networks with instantaneous wall pressures on the cylinder surface as the input variables. For the two-dimensional unsteady flow at Re=60, a fully connected neural network (FCNN) is considered. On the other hand, for a three-dimensional unsteady flow at Re=300 having spanwise variations, we employ two different convolutional neural networks based on an encoder–FCNN (CNN-F) or an encoder–decoder (CNN-D) structure. Numerical simulations are carried out for both Reynolds numbers to obtain instantaneous flow fields, from which the input and output datasets are generated for training these neural networks. At the Reynolds numbers considered, the neural networks constructed accurately predict the transverse velocity fields in the near-wake over the cylinder using the information of instantaneous wall pressures as the input variables. In addition, at Re=300, it is observed that CNN-D shows a better prediction ability than CNN-F.
Enhancing Lithium-Ion Battery Manufacturing Efficiency: A Comparative Analysis Using DEA Malmquist and Epsilon-Based Measures
Authors: Wang Chia-Nan , Yang Fu-Chiang , Vo Nhut T. M. , Nguyen Van Thanh Tien
Published: June 2023

Innovative carbon reduction and sustainability solutions are needed to combat climate change. One promising approach towards cleaner air involves the utilization of lithium-ion batteries (LIB) and electric power vehicles, showcasing their potential as innovative tools for cleaner air. However, we must focus on the entire battery life cycle, starting with production. By prioritizing the efficiency and sustainability of lithium-ion battery manufacturing, we can take an essential step toward mitigating climate change and creating a healthier planet for future generations. A comprehensive case study of the leading LIB manufacturers demonstrates the usefulness of the suggested hybrid methodology. Initially, we utilized the Malmquist model to evaluate these firms’ total efficiency while dissecting their development into technical and technological efficiency change components. We employed the Epsilon-Based Measure (EBM) model to determine each organization’s efficiency and inefficiency scores. The findings show that the EBM approach successfully bridged the gap in the LIB industry landscape. Combined with the Malmquist model, the resulting framework offers a powerful and equitable evaluation paradigm that is easily applicable to any domain. Furthermore, it accurately identifies the top-performing organizations in specific aspects across the research period of 2018–2021. The EBM model demonstrates that most organizations have attained their top level, except for A10, which has superior technology adoption but poor management. A1, A2, A4, A6, A8, A9, and A10 were unable to meet their targets because of the COVID-19 pandemic, despite productivity improvements. A12 leads the three highest-scoring enterprises in efficiency and total productivity changes, while A3 and A5 should focus on innovative production techniques and improved management. The managerial implications provide vital direction for green energy practitioners, enhancing their operational effectiveness. Concurrently, consumers can identify the best LIB manufacturers, allowing them to invest in long-term green energy solutions confidently.
Enhancement of Antibacterial Properties, Surface Morphology and In Vitro Bioactivity of Hydroxyapatite-Zinc Oxide Nanocomposite Coating by Electrophoretic Deposition Technique
Authors: Akram Waseem , Zahid Rumaisa , Usama Raja Muhammad , AlQahtani Salman Ali , Dahshan Mostafa , Basit Muhammad Abdul , Yasir Muhammad
Published: June 2023

To develop medical-grade stainless-steel 316L implants that are biocompatible, non-toxic and antibacterial, such implants need to be coated with biomaterials to meet the current demanding properties of biomedical materials. Hydroxyapatite (HA) is commonly used as a bone implant coating due to its excellent biocompatible properties. Zinc oxide (ZnO) nanoparticles are added to HA to increase its antibacterial and cohesion properties. The specimens were made of a stainless-steel grade 316 substrate coated with HA-ZnO using the electrophoretic deposition technique (EPD), and were subsequently characterized using scanning electron microscopy (SEM), energy dispersive X-ray (EDX), stylus profilometry, electrochemical corrosion testing and Fourier transform infrared (FTIR) spectroscopy. Additionally, cross-hatch tests, cell viability assays, antibacterial assessment and in vitro activity tests in simulated body fluid (SBF) were performed. The results showed that the HA-ZnO coating was uniform and resistant to corrosion in an acceptable range. FTIR confirmed the presence of HA-ZnO compositions, and the in vitro response and adhesion were in accordance with standard requirements for biomedical materials. Cell viability confirmed the viability of cells in an acceptable range (>70%). In addition, the antibacterial activity of ZnO was confirmed on Staphylococcus aureus. Thus, the HA-ZnO samples are recommended for biomedical applications.
35 Years of TFAM Research: Old Protein, New Puzzles
Authors: Kozhukhar Natalya , Alexeyev Mikhail F.
Published: June 2023

Transcription Factor A Mitochondrial (TFAM), through its contributions to mtDNA maintenance and expression, is essential for cellular bioenergetics and, therefore, for the very survival of cells. Thirty-five years of research on TFAM structure and function generated a considerable body of experimental evidence, some of which remains to be fully reconciled. Recent advancements allowed an unprecedented glimpse into the structure of TFAM complexed with promoter DNA and TFAM within the open promoter complexes. These novel insights, however, raise new questions about the function of this remarkable protein. In our review, we compile the available literature on TFAM structure and function and provide some critical analysis of the available data.
Mitochondria Quality Control and Male Fertility
Authors: Costa José , Braga Patrícia C. , Rebelo Irene , Oliveira Pedro F. , Alves Marco G.
Published: June 2023

Mitochondria are pivotal to cellular homeostasis, performing vital functions such as bioenergetics, biosynthesis, and cell signalling. Proper maintenance of these processes is crucial to prevent disease development and ensure optimal cell function. Mitochondrial dynamics, including fission, fusion, biogenesis, mitophagy, and apoptosis, maintain mitochondrial quality control, which is essential for overall cell health. In male reproduction, mitochondria play a pivotal role in germ cell development and any defects in mitochondrial quality can have serious consequences on male fertility. Reactive oxygen species (ROS) also play a crucial role in sperm capacitation, but excessive ROS levels can trigger oxidative damage. Any imbalance between ROS and sperm quality control, caused by non-communicable diseases or environmental factors, can lead to an increase in oxidative stress, cell damage, and apoptosis, which in turn affect sperm concentration, quality, and motility. Therefore, assessing mitochondrial functionality and quality control is essential to gain valuable insights into male infertility. In sum, proper mitochondrial functionality is essential for overall health, and particularly important for male fertility. The assessment of mitochondrial functionality and quality control can provide crucial information for the study and management of male infertility and may lead to the development of new strategies for its management.
YES1 Kinase Mediates the Membrane Removal of Rescued F508del-CFTR in Airway Cells by Promoting MAPK Pathway Activation via SHC1
Authors: Barros Patrícia , Matos Ana M. , Matos Paulo , Jordan Peter
Published: June 2023

Recent developments in CFTR modulator drugs have had a significant transformational effect on the treatment of individuals with Cystic Fibrosis (CF) who carry the most frequent F508del-CFTR mutation in at least one allele. However, the clinical effects of these revolutionary drugs remain limited by their inability to fully restore the plasma membrane (PM) stability of the rescued mutant channels. Here, we shed new light on the molecular mechanisms behind the reduced half-life of rescued F508del-CFTR at the PM of airway cells. We describe that YES1 protein kinase is enriched in F508del-CFTR protein PM complexes, and that its interaction with rescued channels is mediated and dependent on the adaptor protein YAP1. Moreover, we show that interference with this complex, either by depletion of one of these components or inhibiting YES1 activity, is sufficient to significantly improve the abundance and stability of modulator-rescued F508del-CFTR at the surface of airway cells. In addition, we found that this effect was mediated by a decreased phosphorylation of the scaffold protein SHC1, a key regulator of MAPK pathway activity. In fact, we showed that depletion of SHC1 or inhibition of MAPK pathway signaling was sufficient to improve rescued F508del-CFTR surface levels, whereas an ectopic increase in pathway activation downstream of SHC1, through the use of a constitutively active H-RAS protein, abrogated the stabilizing effect of YES1 inhibition on rescued F508del-CFTR. Taken together, our findings not only provide new mechanistic insights into the regulation of modulator-rescued F508del-CFTR membrane stability, but also open exciting new avenues to be further explored in CF research and treatment.
Inhibitory Potential of Synthetic Amino Acid Derivatives against Digestive Enzymes as Promising Hypoglycemic and Anti-Obesity Agents
Authors: Silva Franciane Campos da , Santos Bruna Celeida Silva , Castro Pedro Pôssa de , Amarante Giovanni Wilson , Sousa Orlando Vieira de
Published: June 2023

Over the last decades, the increased incidence of metabolic disorders, such as type two diabetes and obesity, has motivated researchers to investigate new enzyme inhibitors. In this study, the inhibitory effects of synthetic amino acid derivatives (PPC80, PPC82, PPC84, PPC89, and PPC101) on the activity of digestive enzymes were assessed using in vitro assays. The inhibitory effect was determined by the inhibition percentage and the 50% inhibitory concentration (IC50), and the mechanism of action was investigated using kinetic parameters and Lineweaver–Burk plots. PPC80, PPC82, and PPC84 inhibited pancreatic lipase (IC50 of 167–1023 µM) via competitive or mixed mechanisms. The activity of pancreatic α-amylase was suppressed by PPC80, PPC82, PPC84, PPC89, and PPC101 (IC50 of 162–519 µM), which acted as competitive or mixed inhibitors. Finally, PPC84, PPC89, and PPC101 also showed potent inhibitory effects on α-glucosidase (IC50 of 51–353 µM) as competitive inhibitors. The results suggest that these synthetic amino acid derivatives have inhibitory potential against digestive enzymes and may be used as therapeutic agents to control metabolic disorders.
Language Network Connectivity of Euthymic Bipolar Patients Is Altered at Rest and during a Verbal Fluency Task
Authors: Romeo Zaira , Marino Marco , Mantini Dante , Angrilli Alessandro , Spironelli Chiara
Published: June 2023

Abnormalities of the Language Network (LN) have been found in different psychiatric conditions (e.g., schizophrenia and bipolar disorder), supporting the hypothesis that language plays a central role in a high-level integration/connectivity of second-level cognitive processes and the underlying cortical regions. This view implies a continuum of shared neural alterations along the psychotic disorder spectrum. In particular, bipolar disorder (BD) patients were recently documented to have an altered LN asymmetry during resting state. The extent to which the LN architecture is altered and stable also during a language task has yet to be investigated. To address this question, we analyzed fMRI data recorded during an open-eyes resting state session and a silent verbal fluency task in 16 euthymic BD patients and 16 matched healthy controls (HC). Functional connectivity in the LN of both groups was computed using spatial independent component analysis, and group comparisons were carried out to assess the network organization during both rest and active linguistic task conditions. The LN of BD patients involved left and right brain areas during both resting state and linguistic task. Compared to the left-lateralized network found in HC, the BD group was characterized by two anterior clusters (in left frontal and right temporo-insular regions) and the disengagement of the posterior language areas, especially during the verbal fluency task. Our findings support the hypothesis that reduced language lateralization may represent a biological marker across different psychotic disorders and that the altered language network connectivity found at rest in bipolar patients is stable and pervasive as it is also impaired during a verbal fluency task.
Transplacental Transfer of SARS-CoV-2 Receptor-Binding Domain IgG Antibodies from Mothers to Neonates in a Cohort of Pakistani Unvaccinated Mothers
Authors: Harakeh Steve , Khan Ihsan Alam , Rani Gulab Fatima , Ibrahim Muhammad , Khan Aysha Sarwar , Almuhayawi Mohammed , Al-Raddadi Rajaa , Teklemariam Addisu D. , Hazzazi Mohannad S. , Bawazir Waleed M. , Niyazi Hanouf A. , Alamri Turki , Niyazi Hatoon A. , Yousafzai Yasar Mehmood
Published: June 2023

The presence of COVID-19 antibodies in the maternal circulation is assumed to be protective for newborns against SARS-CoV-2 infection. We investigated whether maternal COVID-19 antibodies crossed the transplacental barrier and whether there was any difference in the hematological parameters of neonates born to mothers who recovered from COVID-19 during pregnancy. The cross-sectional study was conducted at the Saidu Group of Teaching Hospitals, located in Swat, Khyber Pakhtunkhwa. After obtaining written informed consent, 115 healthy, unvaccinated mother-neonate dyads were included. A clinical history of COVID-19-like illness, laboratory-confirmed diagnosis, and contact history were obtained. Serum samples from mothers and neonates were tested for SARS-CoV-2 anti-receptor-binding domain (anti-RBD) IgG antibodies. Hematological parameters were assessed with complete blood counts (CBC) and peripheral blood smear examinations. The study population consisted of 115 mothers, with a mean age of 29.44 ± 5.75 years, and most women (68/115 (59.1%)) were between 26 and 35 years of age. Of these mothers, 88/115 (76.5 percent) tested positive for SARS-CoV-2 anti-RBD IgG antibodies, as did 83/115 (72.2 percent) neonatal cord blood samples. The mean levels of SARS-CoV-2 IgG antibodies in maternal and neonatal blood were 19.86 ± 13.82 (IU/mL) and 16.16 ± 12.90 (IU/mL), respectively, indicating that maternal antibodies efficiently crossed the transplacental barrier with an antibody transfer ratio of 0.83. The study found no significant difference in complete blood count (CBC) parameters between seropositive and seronegative mothers, nor between neonates born to seropositive and seronegative mothers.
Role of Epicardial Adipose Tissue Secretome on Cardiovascular Diseases
Authors: Leo Sara , Tremoli Elena , Ferroni Letizia , Zavan Barbara
Published: June 2023

Obesity and insulin resistance are associated with the inflamed and defective adipose tissue (AT) phenotype, and are established risk factors for cardiovascular diseases (CVDs). Extracellular vesicles (EVs) are a heterogeneous group of cell-derived lipid membrane vesicles involved in the onset and development of many pathologies, including insulin resistance, diabetes, and CVDs. The inflammation associated with overweight and obesity triggers the transition of the AT secretome from healthy to pathological, with a consequent increased expression of pro-inflammatory mediators. Epicardial adipose tissue (EAT) is a specialized fat depot that surrounds the heart, in direct contact with the myocardium. Recently, the role of EAT in regulating the physiopathology of many heart diseases has been increasingly explored. In particular, the EAT phenotype and derived EVs have been associated with the onset and exacerbation of CVDs. In this review, we will focus on the role of the AT secretome in the case of CVDs, and will discuss the beneficial effects of EVs released by AT as promising therapeutic candidates.
Drawer Algorithm: A New Metaheuristic Approach for Solving Optimization Problems in Engineering
Authors: Trojovská Eva , Dehghani Mohammad , Leiva Víctor
Published: June 2023

Metaheuristic optimization algorithms play an essential role in optimizing problems. In this article, a new metaheuristic approach called the drawer algorithm (DA) is developed to provide quasi-optimal solutions to optimization problems. The main inspiration for the DA is to simulate the selection of objects from different drawers to create an optimal combination. The optimization process involves a dresser with a given number of drawers, where similar items are placed in each drawer. The optimization is based on selecting suitable items, discarding unsuitable ones from different drawers, and assembling them into an appropriate combination. The DA is described, and its mathematical modeling is presented. The performance of the DA in optimization is tested by solving fifty-two objective functions of various unimodal and multimodal types and the CEC 2017 test suite. The results of the DA are compared to the performance of twelve well-known algorithms. The simulation results demonstrate that the DA, with a proper balance between exploration and exploitation, produces suitable solutions. Furthermore, comparing the performance of optimization algorithms shows that the DA is an effective approach for solving optimization problems and is much more competitive than the twelve algorithms against which it was compared to. Additionally, the implementation of the DA on twenty-two constrained problems from the CEC 2011 test suite demonstrates its high efficiency in handling optimization problems in real-world applications.
Habits, Goals, and Behavioral Signs of Cognitive Perseveration on Wisconsin Card-Sorting Tasks
Authors: Kopp Bruno , Al-Hafez Bilal , Steinke Alexander
Published: June 2023

Wisconsin card-sorting tasks provide unique opportunities to study cognitive flexibility and its limitations, which express themselves behaviorally as perseverative errors (PE). PE refer to those behavioral errors on Wisconsin card-sorting tasks that are committed when cognitive rules are maintained even though recently received outcomes demand to switch to other rules (i.e., cognitive perseveration). We explored error-suppression effects (ESE) across three Wisconsin card-sorting studies. ESE refer to the phenomenon that PE are reduced on repetitive trials compared to non-repetitive trials. We replicated ESE in all three Wisconsin card-sorting studies. Study 1 revealed that non-associative accounts of ESE, in particular the idea that cognitive inhibition may account for them, are not tenable. Study 2 suggested that models of instrumental learning are among the most promising associative accounts of ESE. Instrumental learning comprises goal-directed control and the formation of corresponding associative memories over and above the formation of habitual memories according to dual-process models of instrumental learning. Study 3 showed that cognitive, rather than motor, representations of responses should be conceptualized as elements entering goal-directed instrumental memories. Collectively, the results imply that ESE on Wisconsin card-sorting tasks are not only a highly replicable phenomenon, but they also indicate that ESE provide an opportunity to study cognitive mechanisms of goal-directed instrumental control. Based on the reported data, we present a novel theory of cognitive perseveration (i.e., the ‘goal-directed instrumental control’ GIC model), which is outlined in the Concluding Discussion.
Evaluating Influencing Factors of Audiences’ Attitudes toward Virtual Concerts: Evidence from China
Authors: Deng Jing , Pan Younghwan
Published: June 2023

The purpose of this study is to investigate and validate the influencing factors of audiences’ attitudes toward virtual concerts. In order to address this issue, the current study proposes a conceptual model integrating player experience factors (autonomy, relatedness, and engagement) and the technology acceptance model (perceived usefulness, perceived ease of use, and perceived enjoyment). An online questionnaire on virtual concert experiences was conducted among Chinese audiences who had attended virtual concerts previously. Structural equation modeling was then used to establish the relationships between variables. The results suggested that autonomy, relatedness, and engagement positively impacted perceived usefulness, perceived ease of use, and perceived enjoyment. Furthermore, the perceived usefulness, perceived ease of use, and perceived enjoyment were significant predictors of audiences’ attitudes. The findings of this study could provide a reference for relevant virtual entertainment providers and could also serve as a point of development and exploration for the technology acceptance model and player experience in the field of virtual concerts.
Alternative Lengthening of Telomeres in Pediatric High-Grade Glioma and Therapeutic Implications
Authors: Umaru Banlanjo , Sengupta Satarupa , Senthil Kumar Shiva , Drissi Rachid
Published: June 2023

Pediatric high-grade gliomas (pHGGs), including diffuse intrinsic pontine glioma (DIPG), are highly aggressive tumors with dismal prognoses despite multimodal therapy including surgery, radiation therapy, and chemotherapy. To achieve cellular immortality cancer cells must overcome replicative senescence and apoptosis by activating telomere maintenance mechanisms (TMMs) through the reactivation of telomerase activity or using alternative lengthening of telomere (ALT) pathways. Although the ALT phenotype is more prevalent in pHGGs compared to adult HGGs, the molecular pathway and the prognostic significance of ALT activation are not well understood in pHGGs. Here, we report the heterogeneity of TMM in pHGGs and their association with genetic alterations. Additionally, we show that sensitivity to the protein kinase ataxia telangiectasia- and RAD3-related protein (ATR) inhibitor and the ATR downstream target CHK1 is not specific to pHGG ALT-positive cells. Together, these findings underscore the need for novel therapeutic strategies to target ALT in pHGG tumors.
CD28 and 41BB Costimulatory Domains Alone or in Combination Differentially Influence Cell Surface Dynamics and Organization of Chimeric Antigen Receptors and Early Activation of CAR T Cells
Authors: Mezősi-Csaplár Marianna , Szöőr Árpád , Vereb György
Published: June 2023

Chimeric antigen receptor (CAR)-modified T cells brought a paradigm shift in the treatment of chemotherapy-resistant lymphomas. Conversely, clinical experience with CAR T cells targeting solid tumors has been disheartening, indicating the necessity of their molecular-level optimization. While incorporating CD28 or 41BB costimulatory domains into CARs in addition to the CD3z signaling domain improved the long-term efficacy of T cell products, their influence on early tumor engagement has yet to be elucidated. We studied the antigen-independent self-association and membrane diffusion kinetics of first- (.z), second- (CD28.z, 41BB.z), and third- (CD28.41BB.z) generation HER2-specific CARs in the resting T cell membrane using super-resolution AiryScan microscopy and fluorescence correlation spectroscopy, in correlation with RoseTTAFold-based structure prediction and assessment of oligomerization in native Western blot. While .z and CD28.z CARs formed large, high-density submicron clusters of dimers, 41BB-containing CARs formed higher oligomers that assembled into smaller but more numerous membrane clusters. The first-, second-, and third-generation CARs showed progressively increasing lateral diffusion as the distance of their CD3z domain from the membrane plane increased. Confocal microscopy analysis of immunological synapses showed that both small clusters of highly mobile CD28.41BB.z and large clusters of less mobile .z CAR induced more efficient CD3ζ and pLck phosphorylation than CD28.z or 41BB.z CARs of intermediate mobility. However, electric cell-substrate impedance sensing revealed that the CD28.41BB.z CAR performs worst in sequential short-term elimination of adherent tumor cells, while the .z CAR is superior to all others. We conclude that the molecular structure, membrane organization, and mobility of CARs are critical design parameters that can predict the development of an effective immune synapse. Therefore, they need to be taken into account alongside the long-term biological effects of costimulatory domains to achieve an optimal therapeutic effect.
Preparation-Height-Specific Surface Area of Flower-like ZnO for Norfloxacin Removal in Dialysis Wastewater
Authors: Hsiao Shui-Shu , Nguyen Thi-Minh-Phuong , Wang Li-Pang , Hong Gui-Bing , Cheng Yu-Chen , Wang Pei-Hua , Duong Cong-Chinh , Yang Chia-Jui
Published: June 2023

According to statistics, the number of patients with kidney dialysis is increasing every year, especially in Taiwan. The high content of antibiotics in kidney dialysis wastewater can create an environmental burden if not properly treated. Therefore, in this study, a new design for a flower-like ZnO catalyst that can effectively treat norfloxacin (NF) in dialysis wastewater is presented and its NF treatment efficiency is investigated under different operating parameters (including different dosages, pH, ambient temperatures, and pollutant concentrations). Scanning electron microscope results indicate that the ZnO sample consists of flower-like nanostructures with diameters of about 4.97 μm. The surface area, pore volume, and pore size of the flower-like ZnO were estimated to be 46.45 m2g−1, 0.132 cm3g−1, and 19.50 nm, respectively. The total removal efficiency increased with the increase in the initial pH value of NF, when the initial pH value of NF increased from 3.5 (38.1%) to 7.5 (82.5%). However, the total removal efficiency decreased with an increase in the initial pH value of NF, when the initial pH value of NF increased from 9.5 (79.5%) to 11.5 (32.9%). The second-order kinetic simulation results show that the linear relationship is better than that of the first-order kinetic, and the Rc2 values are all above 0.9.
Horizontal Transfer of Malignant Traits and the Involvement of Extracellular Vesicles in Metastasis
Authors: Arena Goffredo O. , Forte Stefano , Abdouh Mohamed , Vanier Cheryl , Corbeil Denis , Lorico Aurelio
Published: June 2023

Metastases are responsible for the vast majority of cancer deaths, yet most therapeutic efforts have focused on targeting and interrupting tumor growth rather than impairing the metastatic process. Traditionally, cancer metastasis is attributed to the dissemination of neoplastic cells from the primary tumor to distant organs through blood and lymphatic circulation. A thorough understanding of the metastatic process is essential to develop new therapeutic strategies that improve cancer survival. Since Paget’s original description of the “Seed and Soil” hypothesis over a hundred years ago, alternative theories and new players have been proposed. In particular, the role of extracellular vesicles (EVs) released by cancer cells and their uptake by neighboring cells or at distinct anatomical sites has been explored. Here, we will outline and discuss these alternative theories and emphasize the horizontal transfer of EV-associated biomolecules as a possibly major event leading to cell transformation and the induction of metastases. We will also highlight the recently discovered intracellular pathway used by EVs to deliver their cargoes into the nucleus of recipient cells, which is a potential target for novel anti-metastatic strategies.
A Gluten-Free Diet during Pregnancy and Early Life Increases Short Chain Fatty Acid-Producing Bacteria and Regulatory T Cells in Prediabetic NOD Mice
Authors: Johansen Valdemar Brimnes Ingemann , Færø Daisy , Buschard Karsten , Kristiansen Karsten , Pociot Flemming , Kiilerich Pia , Josefsen Knud , Haupt-Jorgensen Martin , Antvorskov Julie Christine
Published: June 2023

The incidence of the autoimmune disease type 1 diabetes is increasing, likely caused by environmental factors. A gluten-free diet has previously been shown to ameliorate autoimmune diabetes in non-obese diabetic (NOD) mice and humans. Although the exact mechanisms are not understood, interventions influencing the intestinal microbiota early in life affect the risk of type 1 diabetes. Here, we characterize how NOD mice that are fed a gluten-free (GF) diet differ from NOD mice that are fed a gluten-containing standard (STD) diet in terms of their microbiota composition by 16S rRNA gene amplicon sequencing and pancreatic immune environment by real-time quantitative PCR at the prediabetic stage at 6 and 13 weeks of age. Gut microbiota analysis revealed highly distinct microbiota compositions in both the cecum and the colon of GF-fed mice compared with STD-fed mice. The microbiotas of the GF-fed mice were characterized by an increased Firmicutes/Bacteroidetes ratio, an increased abundance of short chain fatty acid (particularly butyrate)-producing bacteria, and a reduced abundance of Lactobacilli compared with STD mice. We found that the insulitis score in the GF mice was significantly reduced compared with the STD mice and that the markers for regulatory T cells and T helper 2 cells were upregulated in the pancreas of the GF mice. In conclusion, a GF diet during pre- and early post-natal life induces shifts in the cecal and colonic microbiota compatible with a less inflammatory environment, providing a likely mechanism for the protective effect of a GF diet in humans.
Circulating Tumor DNA in the Management of Early-Stage Breast Cancer
Authors: Vlataki Katerina , Antonouli Sevastiani , Kalyvioti Christina , Lampri Evangeli , Kamina Sevasti , Mauri Davide , Harissis Haralampos V. , Magklara Angeliki
Published: June 2023

Liquid biopsies refer to the isolation and analysis of tumor-derived biological material from body fluids, most commonly blood, in order to provide clinically valuable information for the management of cancer patients. Their non-invasive nature allows to overcome the limitations of tissue biopsy and complement the latter in guiding therapeutic decision-making. In the past years, several studies have demonstrated that circulating tumor DNA (ctDNA) detection can be used in the clinical setting to improve patient prognosis and monitor therapy response, especially in metastatic cancers. With the advent of significant technological advances in assay development, ctDNA can now be accurately and reliably identified in early-stage cancers despite its low levels in the bloodstream. In this review, we discuss the most important studies that highlight the potential clinical utility of ctDNA in early-stage breast cancer focusing on early diagnosis, detection of minimal residual disease and prediction of metastatic relapse. We also offer a concise description of the most sensitive techniques that are deemed appropriate for ctDNA detection in early-stage cancer and we examine their advantages and disadvantages, as they have been employed in various studies. Finally, we discuss future perspectives on how ctDNA could be better integrated into the everyday oncology practice.
Ammonia Sensor Based on Co2+/SCN− Modified Core-Shell MCM-41 for Rapid Naked-Eye Colorimetric Detection
Authors: Liu Songtao , Wei Kaixin , Cheng Fuqiang , Li Yongsheng , Xue Min
Published: June 2023

Mesoporous silica materials have been widely used as gas adsorbents due to their excellent adsorption ability, large specific surface area, simple preparation process, and convenient functionalization. In this study, a core–shell mesoporous material MCM-41@SiO2 was synthesized and functionalized by CoCl2, and subsequently KSCN, to prepare an ammonia adsorbent. The adsorbent was proved to possess high surface area, good sphericity, uniform size, good dispersibility, and high adsorption capability following DLS, SEM, TEM, and a static adsorption study. Moreover, the successful functionalization and thermal stability were confirmed by FT-IR, XPS, and TGA. The material was then used to fabricate a glass tube sensor for the rapid naked-eye detection of ammonia gas. The sensor showed good performance in terms of sensing speed, selectivity, accuracy, and reusability. Within 5 s, NH3 could be detected by the discoloration, and a wide NH3 concentration from 20 to 1000 ppm could be detected. It also showed a good linear relationship between discoloration length and ammonia concentration. A reversible color change from blue to yellow indicated the presence of NH3, which was attributed to the formation and disassembly of ammonia-containing complex [Co(NH3)6(NCS)2]. With fast adsorption, naked-eye sensing properties, and good selectivity, the sensor holds good promise for indoor NH3 sensing and other more complicated situations.
Hazardous Child Labour, Psychosocial Functioning, and School Dropouts among Children in Bangladesh: A Cross-Sectional Analysis of UNICEF’s Multiple Indicator Cluster Surveys (MICS)
Authors: Thi Aye Myat , Zimmerman Cathy , Ranganathan Meghna
Published: June 2023

Child labour is a common financial coping strategy in poor households, especially in low-and middle-income countries with many children working under hazardous conditions. Little is known about the linkages between hazardous work conditions and psycho-social and educational outcomes. We analysed the Bangladesh Multiple Indicator Cluster Survey (BMICS) round 6 to assess the association between the exposure variables, including child labour, hazardous child labour (HZCL) and hazardous work, and outcome variables, including psychosocial functioning difficulty and school dropout, in children aged 5 to 17 years. We conducted bivariable and multivariable analyses to examine the association. In the adjusted analyses, children engaged in HZCL had increased odds of psychosocial functioning difficulty (aOR: 1.41; 95% CI: 1.16–1.72) and school dropout (aOR: 5.65; 95% CI: 4.83–6.61) among 5–14-year-olds compared to children who did not engage in child labour and hazardous work. Other independent factors associated with psychosocial functioning difficulty and school dropout included being male, living in a deprived neighbourhood, being exposed to violent punishment, the caregiver’s attitude towards physical punishment, the mother’s functional difficulty and lower maternal education. The linkages between hazardous work and psychosocial functioning difficulty appear more prominent among children not in school. Further, the evidence on the relationship between hazardous work and school dropout is stronger among children with psychosocial functioning difficulty. Policies and programmes that target the most hazardous forms of work are likely to have the greatest benefits for children’s mental health, social well-being and educational attainment.
Multiple Sclerosis—Related Dietary and Nutritional Issues: An Updated Scoping Review with a Focus on Pediatrics
Authors: Mandato Claudia , Colucci Angelo , Lanzillo Roberta , Staiano Annamaria , Scarpato Elena , Schiavo Luigi , Operto Francesca Felicia , Serra Maria Rosaria , Di Monaco Cristina , Napoli Julia Sara , Massa Grazia , Vajro Pietro
Published: June 2023

Purpose. Lifestyle/dietetic habits play an important role in the development and progression of multiple sclerosis (MS) disease. Here, we examine the basic pathomechanisms underlying intestinal and brain barrier modifications in MS and consider diets and dietary supplementations proposed over time to complement pharmacological therapies for improving disease outcome both in adults and in children. Methods. Scoping literature search about evidence-based findings in MS-related gut-brain axis (GBA) pathophysiology and nutritional issues at all ages. Findings. Data show that (1) no universal best diet exists, (2) healthy/balanced diets are, however, necessary to safeguard the adequate intake of all essential nutrients, (3) diets with high intakes of fruits, vegetables, whole grains, and lean proteins that limit processed foods, sugar, and saturated fat appear beneficial for their antioxidant and anti-inflammatory properties and their ability to shape a gut microbiota that respects the gut and brain barriers, (4) obesity may trigger MS onset and/or its less favorable course, especially in pediatric-onset MS. Vitamin D and polyunsaturated fatty acids are the most studied supplements for reducing MS-associated inflammation. Conclusions. Pending results from other and/or newer approaches targeting the GBA (e.g., pre- and probiotics, engineered probiotics, fecal-microbiota transplantation), accurate counseling in choosing adequate diet and maintaining physical activity remains recommended for MS prevention and management both in adults and children.
Climate Change Adaptation and the Agriculture–Food System in Myanmar
Authors: Tun Oo Aung , Boughton Duncan , Aung Nilar
Published: June 2023

The agricultural sector provides employment and income to the majority of Myanmar’s population. The sector, however, is extremely susceptible to severe weather, rising temperatures, and changes in precipitation. A lack of knowledge about farming communities’ climate change vulnerabilities, as well as the insufficient integration of policies and programs, is a constraint to climate change adaptation in agriculture sectors. This paper analyzes the drivers of the agricultural sector’s vulnerability to climate change and highlights the key production systems that are most at risk in Myanmar. The paper examines historical climate information and the anticipated effects of climate change. We include an in-depth literature review and summaries of climate change adaptation efforts in agriculture sectors, along with recommendations for targeted, locally appropriate actions to strengthen the resilience of the agricultural sector. Farm households use a combination of scientific and indigenous adaptation strategies to cope with the effects of climate change. Additionally, the study reviews Myanmar’s institutional framework for climate action and government priorities for adaptation measures, emphasizes the urgent need for climate action in agriculture sectors, and calls for more research and development efforts on the effects of climate change on rural livelihoods and agriculture.
Is the Proof in the Pain? Association between Head and Neck Pain and Vessel Pathology at Follow-Up in Cervical Artery Dissection: A Retrospective Data Analysis
Authors: Baumann Jil , Stattmann Miranda , Wegener Susanne
Published: June 2023

Unilateral head and neck pain is a hallmark of cervical artery dissection (CAD). While pain is conceived as an alarming sign for patients and often leads to discovery of the dissection, it is not known if persistence of pain is associated with the course of CAD. Potentially, pain could indicate persisting vessel pathology and thus guide treatment decisions aimed at reducing risk of ischemic stroke in CAD. We performed a retrospective analysis of data from patients with CAD treated at the University Hospital Zurich (USZ). Only patients with information about the presence of pain, independence after CAD according to the modified Rankin scale (mRS), and imaging-based information on vessel status were included. Patients were grouped according to presence/absence of head and/or neck pain on admission and at a three-month follow-up. We used descriptive statistics and logistic regression to reveal a potential association between pain on admission and pain at follow-up with status of the dissected vessel at follow-up (open vs. stenosed or occluded). We screened 139 patients with CAD between 2014 and 2019 and included 68. Fifty-nine patients (86.8%) had pain on admission, which was resolved in 46 (68%) at follow-up. Our post hoc analysis revealed that more patients with headache or neck pain on admission had a migraine diagnosis in medical history (n = 7 (10.4%) vs. n = 0 (0%), p = 0.029) and that NIHSS on admission was higher in patients with no pain at presentation (group B NIHSS = 3, IQR 8 vs. group A NIHSS = 2, IQR 5, group C NIHSS = 0, IQR 2, p = 0.041). There were no other differences between the three patient groups in the descriptive analysis. Logistic regression analysis for vessel status at follow-up did not show an association with pain on admission or at follow-up. In our cohort of patients with CAD, headache was a common initial clinical presentation, which rarely persisted for three months. Headache on admission or at follow-up did not predict persisting vessel pathology in patients with CAD.