bluuebunny/crossref_metadata_2025 huggingface dataset:
Addison’s Disease in the Course of Recurrent Microangiopathic Antiphospholipid Syndrome—A Clinical Presentation and Review of the Literature
Authors: Grabarczyk Małgorzata , Gorczyca Marta , Cieślik Paweł , Hrycek Antoni , Holecki Michał
Published: December 2022

The article presents a male patient with adrenocortical insufficiency in the course of antiphospholipid syndrome (APS). It also describes recurrent exacerbations of his clinical status, characteristic of microangiopathic antiphospholipid syndrome (MAPS) which had been misdiagnosed as a disseminated intravascular coagulopathy (DIC) syndrome due to sepsis with multi-organ failure, including heart, kidneys, and liver. Issues related to pathogenesis, clinical symptoms, differential diagnosis, and treatment of APS in the context of presently distinguished subtypes of this syndrome have been addressed. The role of vascular endothelial cell activation and the influence of coagulation patterns on the development of APS continuum clinical symptoms have also been mentioned. In addition, this paper highlights that the diagnosis of APS should be considered in patients with adrenal insufficiency and abdominal pain, even without any prior history of thromboembolic diseases, as well as in the course of DIC, especially without predisposing factors.
Antibiotic Resistance of Helicobacter pylori in Patients with Peptic Ulcer
Authors: Vu Thanh Binh , Tran Thi Nhu Quynh , Tran Thi Quynh Anh , Vu Dinh Luong , Hoang Van Thuan
Published: December 2022

Background and Objectives: To determine the antibiotic resistance rate of H. pylori among patients with peptic ulcer. Materials and Methods: A cross-sectional monocentric study was conducted from January to December 2021 among patients aged from 16 years with gastrointestinal symptoms and esophagogastroduodenoscopy. Gastric mucosa biopsies were collected at the edges of the ulcer or at lesion sites for H. pylori culture. Five antibiotics (amoxicillin (AMX), clarithromycin (CLR), metronidazole (MTZ), levofloxacin (LEV), and tetracycline (TET)) were selected for antibiotic susceptibility testing. Results: One hundred and twenty-five patients were included, and the sex ratio was 0.6. Their mean age was 47.3 ± 14.2 years. All of the participants had gastritis, and 24.0% had duodenitis. A total of 21.6% of patients had a duodenal ulcer, and 12.8% had an antral ulcer. A total of 40 specimens have grown in H. pylori culture. The proportion of resistance to AMX, CLR, MTZ, LEV, and TET was 27.5%, 50%, 67.5%, 35%, and 5%, respectively. The proportion of multidrug resistance was 22.5%. The proportion of double resistance to AMX + CLR was 20.0%, AMX + MTZ was 15.0%, AMX + LEV was 2.5%, CLR + MTZ was 32.5%, and TET + MTZ was 5.0%. Conclusions: Our research results show that the treatment with MTX-TET or LVX-AMOX has the highest sensitivity rate. Therefore, practitioners should refer to these regimes to eradicate H. pylori in patients with gastric and duodenal ulcers. The reports on H. pylori eradication from different geographic areas show heterogeneous results. Therefore, continuous monitoring of antibiotic resistance of H. pylori in each population is very important. Having evidence helps clinicians to treat patients most effectively, reduce treatment costs, and limit the rate of antibiotic resistance.
Osteoarthritis of the Temporomandibular Joint: A Narrative Overview
Authors: Mélou Caroline , Pellen-Mussi Pascal , Jeanne Sylvie , Novella Agnès , Tricot-Doleux Sylvie , Chauvel-Lebret Dominique
Published: December 2022

Background and Objectives: This study reviewed the literature to summarize the current and recent knowledge of temporomandibular joint osteoarthritis (TMJOA). Methods: Through a literature review, this work summarizes many concepts related to TMJOA. Results: Although many signaling pathways have been investigated, the etiopathogenesis of TMJOA remains unclear. Some clinical signs are suggestive of TMJOA; however, diagnosis is mainly based on radiological findings. Treatment options include noninvasive, minimally invasive, and surgical techniques. Several study models have been used in TMJOA studies because there is no gold standard model. Conclusion: More research is needed to develop curative treatments for TMJOA, which could be tested with reliable in vitro models, and to explore tissue engineering to regenerate damaged temporomandibular joints.
Innovative C-Arm-Free Navigation Technique for Posterior Spinal Fixation for Atlantoaxial Subluxation: A Technical Note
Authors: Tanaka Masato , Sake Naveen , Kim Dae-Geun , Arataki Shinya , Desai Dhvanit , Fujiwara Yoshihiro , Yamauchi Taro
Published: December 2022

Study design: Technical note. Objectives: To present a novel C-arm-free technique guided by navigation to insert and place a C1 lateral mass screw. Background and Objectives: Atlantoaxial subluxation (AAS) is a relatively common sequelae in patients with rheumatoid arthritis (RA) and upper cervical trauma. If they present with severe symptoms, surgical intervention such as posterior fusion is indicated. The established treatment for AAS is fixation with a C1 lateral mass screw and C2 pedicle screw (modified Goel technique) to achieve bony fusion. However, this technique requires fluoroscopy for C1 screw insertion. To avoid exposing the operating team to radiation, we present here a novel C-arm-free C1 lateral mass screw insertion technique for AAS. Materials and Methods: A 67-year-old man was referred to our hospital with neck pain, quadriparesis, and clumsiness and numbness of both upper and lower limbs. He had undergone C3–6 posterior fusion previously in another hospital. In physical examination, he had severe muscle weakness of bilateral upper limbs and hypoesthesia of all four limbs. He had hyper-reflexia of bilateral lower limbs and pollakiuria. His Japanese orthopedic score was 8 points out of 17. Preoperative radiograms showed AAS with an atlantodental interval (ADI) of 7 mm. MRI indicated retro-odontoid pseudotumor and severe spinal cord compression at the C1–2 level. The patient underwent posterior atlantoaxial fixation under navigation guidance. To prevent epidural bleeding during the insertion and placement of a C1 lateral mass screw, we have here defined a novel screw insertion technique. Results: The surgical time was clocked as 127 min and blood loss was 100 mL. There were no complications per-operatively or in the postoperative period. The patient showed almost full recovery (JOA 16/17) at two months follow-up and a solid bony fusion was noticed in the radiograms at one year follow-up. Conclusions: This novel surgical procedure and C1 lateral mas screw placement technique is a practical and safe method in recent advances of AAS treatment. Procedurally, the technique helps prevent epidural bleeding from the screw entry point and also allows for proper C1 screw insertion under navigation guidance without exposing surgeons and staff to the risk of fluoroscopic radiation.
Effect of Sub-Inhibitory Concentrations of Nitrofurantoin, Ciprofloxacin, and Trimethoprim on In Vitro Biofilm Formation in Uropathogenic Escherichia coli (UPEC)
Authors: Whelan Shane , O’Grady Mary Claire , Corcoran Gerard Daniel , Finn Karen , Lucey Brigid
Published: December 2022

The purpose of this study was to determine the effect of sublethal concentrations of nitrofurantoin, ciprofloxacin, and trimethoprim on biofilm formation in 57 uropathogenic Escherichia coli strains (UPEC). The minimum inhibitory concentration of nitrofurantoin, ciprofloxacin, and trimethoprim was determined and the biofilm formation for each isolate with and without sub-lethal concentrations of each antibiotic was then quantified. The statistical significance of changes in biofilm formation was ascertained by way of a Dunnett’s test. A total of 22.8% of strains were induced to form stronger biofilms by nitrofurantoin, 12% by ciprofloxacin, and 19% by trimethoprim; conversely 36.8% of strains had inhibited biofilm formation with nitrofurantoin, 52.6% with ciprofloxacin, and 38.5% with trimethoprim. A key finding was that even in cases where the isolate was resistant to an antibiotic as defined by EUCAST, many were induced to form a stronger biofilm when grown with sub-MIC concentrations of antibiotics, especially trimethoprim, where six of the 22 trimethoprim resistant strains were induced to form stronger biofilms. These findings suggest that the use of empirical treatment with trimethoprim without first establishing susceptibility may in fact potentiate infection in cases where a patient who is suffering from a urinary tract infection (UTI) caused by trimethoprim resistant UPEC is administered trimethoprim. This emphasizes the need for laboratory-guided treatment of UTI.
Features and Possible Applications of Plant Lipid-Binding and Transfer Proteins
Authors: Melnikova Daria N. , Finkina Ekaterina I. , Bogdanov Ivan V. , Tagaev Andrey A. , Ovchinnikova Tatiana V.
Published: December 2022

In plants, lipid trafficking within and inside the cell is carried out by lipid-binding and transfer proteins. Ligands for these proteins are building and signaling lipid molecules, secondary metabolites with different biological activities due to which they perform diverse functions in plants. Many different classes of such lipid-binding and transfer proteins have been found, but the most common and represented in plants are lipid transfer proteins (LTPs), pathogenesis-related class 10 (PR-10) proteins, acyl-CoA-binding proteins (ACBPs), and puroindolines (PINs). A low degree of amino acid sequence homology but similar spatial structures containing an internal hydrophobic cavity are common features of these classes of proteins. In this review, we summarize the latest known data on the features of these protein classes with particular focus on their ability to bind and transfer lipid ligands. We analyzed the structural features of these proteins, the diversity of their possible ligands, the key amino acids participating in ligand binding, the currently known mechanisms of ligand binding and transferring, as well as prospects for possible application.
Evaluation of Concentration Polarization Due to the Effect of Feed Water Temperature Change on Reverse Osmosis Membranes
Authors: Dévora-Isiordia Germán Eduardo , Cásares-De la Torre Cristian Ascención , Morales-Mendívil Deemi Paola , Montoya-Pizeno Rosario , Velázquez-Limón Nicolás , Aguilar-Jiménez Jesús Armando , Ríos-Arriola Juan
Published: December 2022

Water is a necessary resource for life development. Its excessive consumption has a negative impact, generating scarcity problems worldwide. Desalination is an alternative to solve these problems; its objective is to reduce the concentration of total dissolved solids to levels suitable for consumption. The most widely used desalination technology is reverse osmosis, which works by means of semipermeable membranes; however, lack of knowledge or wrong operation cause phenomena such as concentration polarization, which reduces the effective area for mass transfer in the membrane, increasing the energy consumption of the process. The objective of the present study is to evaluate the concentration polarization (β) of the concentration in reverse osmosis membranes by varying the temperature in the feed water (23, 25.5, 28, and 35 °C) for different concentrations (5000 and 10,000 mg L−1) in order to reduce its impact on energy consumption (kWh m−3). The results show that as the temperature increases, the specific energy consumption decreases for both concentrations. In the 5000 mg L−1 tests, the specific energy consumption decreased by 0.590 kWh m−3, representing 12.5%. For 10,000 mg L−1 tests, the specific energy consumption shows a reduction of 0.72 kWh m−3, which represents a percentage decrease of 14.54%.
A Novel Approach of Microstructure Refinement of TiAl in Laser Beam Welding
Authors: Liu Jie , Guo Shun , Zhang Peng , Ma Tao , Wang Zhuo , Wu Tongli , Wang Li , Wang Kehong
Published: December 2022

Grain refinement through borides is known to be suppressed when TiAl is welded with a laser beam. As β grains do not primarily nucleate on boride at a high cooling rate, a mixture of nitrogen and argon is applied as a protecting gas for the formation of TiN during solidification. The phase transformation is changed correspondingly from Liquid → Liquid + β → β → α + β → α + γ+ β → α2 + γ + B2 to Liquid → TiN + Liquid → β+ TiN → α + γ + TiN → α2 + γ+ TiN. It is found that β grains prefer to nucleate heterogeneously on the suspending TiN in the melt with orientation relationship {111}TiN//{110}β, leading to refined β grains. α2 colonies that were thus modified into fine non-dendritic grains. The effects of nitrogen as a shielding atmosphere on the microstructure evolution of TiAl are elaborately studied.
Quantified Approach for Evaluation of Geometry Visibility of Optical-Based Process Monitoring System for Laser Powder Bed Fusion
Authors: Zhang Song , Adjei-Kyeremeh Frank , Wang Hui , Kolter Moritz , Raffeis Iris , Schleifenbaum Johannes Henrich , Bührig-Polaczek Andreas
Published: December 2022

The long-term sustainability of the Additive Manufacturing (AM) industry not only depends on the ability to produce parts with reproducible quality and properties to a large extent but also on the standardization of the production processes. In that regard, online process monitoring and detection of defective parts during production become inevitable. Optical-based process monitoring techniques are popular; however, most work has been mainly focused on capturing images of print abnormalities without taking other influencing factors, such as camera and part position, chamber illumination, and print geometry on the resolution of the captured images, into account. In this work, we present a scenario to evaluate and quantify the performance of an optical-based monitoring system in a Laser Powder Bed Fusion (LPBF) machine using the F1 score, considering factors such as scan vector orientation, part geometry (size) and position in a built chamber with a fixed camera position. The quantified results confirm that the F1 score can be used as a reliable means of evaluating the performance of optical-based monitoring systems in the LPBF process for the purposes of standardization. The biggest line width of the test artifact (1000 µm) had the highest F1 score range of 0.714–0.876 compared to the smallest (200 µm) with a 0.158–0.649 F1 score.
Coumarins of Lovage Roots (Levisticum officinale W.D.J.Koch): LC-MS Profile, Quantification, and Stability during Postharvest Storage
Authors: Olennikov Daniil N.
Published: December 2022

Lovage (Levisticum officinale W.D.J. Koch) is a known aromatic apiaceous species that is widely used as a culinary and medicinal plant. Traditionally, more scientific attention has been paid to lovage volatiles, while other groups of compounds have been underutilized. In this study, metabolites of fresh lovage roots were investigated by liquid chromatography–mass spectrometry, and 25 compounds were identified, including coumarins as basic components and minor hydroxycinnamates; most were detected for the first time in the plant. Four major coumarins (including apterin, xanthotoxin, isopimpinellin, and pimpinellin) were successfully separated by a validated HPLC–PDA method, and the fresh roots of seven lovage cultivars as well as the dry roots of commercial lovage were quantified. The coumarin content deviation was 1.7–2.9 mg/g in the fresh roots and 15–24 mg/g in the dry roots. A variation in the coumarin level was found during storage of the fresh lovage roots at chill and room temperatures, while storage of the dried roots at room temperature showed the lowest loss of target compounds. This new information about the metabolites of lovage indicates the prospects of the plant roots as a source of dietary coumarins.
Hemorheological and Microcirculatory Relations of Acute Pancreatitis
Authors: Kotan Robert , Peto Katalin , Deak Adam , Szentkereszty Zsolt , Nemeth Norbert
Published: December 2022

Acute pancreatitis still means a serious challenge in clinical practice. Its pathomechanism is complex and has yet to be fully elucidated. Rheological properties of blood play an important role in tissue perfusion and show non-specific changes in acute pancreatitis. An increase in blood and plasma viscosity, impairment of red blood cell deformability, and enhanced red blood cell aggregation caused by metabolic, inflammatory, free radical-related changes and mechanical stress contribute to the deterioration of the blood flow in the large vessels and also in the microcirculation. Revealing the significance of these changes in acute pancreatitis may better explain the pathogenesis and optimize the therapy. In this review, we give an overview of the role of impaired microcirculation by changes in hemorheological properties in acute pancreatitis.
Metabolites Associated with the Main Nutrients in Two Varieties of Purple Rice Processed to Polished Rice
Authors: Xiong Qiangqiang , Wang Runnan , Sun Changhui , Wang Ruizhi , Wang Xiaoyu , Zhang Yu , Zhang Hongcheng , Zhu Jinyan
Published: December 2022

Under the same nitrogen fertilizer and cultivation conditions, the nutrients of rice are strongly affected during the processing of brown rice to polished rice, especially in special rice varieties. In this study, twenty-two amino acids in brown and polished rice of two purple rice varieties were quantified using targeted metabolomics, and the relationships among the main nutrients, mineral elements and metabolites were analysed. The results showed that the amino acid levels in YZN1_H (polished rice of Yangzinuo No. 1) and YZN2_H (polished rice of Yangzinuo No. 2) decreased to different degrees compared with those in YZN1_B (brown rice of Yangzinuo No. 1) and YZN2_B (brown rice of Yangzinuo No. 2). Citric acid is closely associated with amino acids. The total sugar (TS), ATP, and soluble dietary fiber (SDF) levels in YZN1_B decreased by 9.37%, 53.85%, and 75.71%, respectively, compared with those in YZN1_H. The TS, ATP, and SDF levels in YZN2_B decreased significantly by 6.92%, 21.03%, and 76.78%, respectively, compared with those in YZN2_H. Citric acid was significantly negatively correlated with ATP and SDF but significantly positively correlated with carotenoids. The Se content in YZN1_H was significantly higher than that in YZN1_B by 87.02%. The Se content in YZN2_H was significantly higher than that in YZN2_B by 72.02%. Citric acid was significantly positively correlated with Fe, Mn, Ca, and Mg. Citric acid was identified as a candidate key metabolite that affects changes in the main nutrients in purple rice during processing.
Plotter Cut Stencil Masks for the Deposition of Organic and Inorganic Materials and a New Rapid, Cost Effective Technique for Antimicrobial Evaluations
Authors: Childs Andre , Pereira Jorge , Didier Charles M. , Baksh Aliyah , Johnson Isaac , Castro Jorge Manrique , Davidson Edwin , Santra Swadeshmukul , Rajaraman Swaminathan
Published: December 2022

Plotter cutters in stencil mask prototyping are underutilized but have several advantages over traditional MEMS techniques. In this paper we investigate the use of a conventional plotter cutter as a highly effective benchtop tool for the rapid prototyping of stencil masks in the sub-250 μm range and characterize patterned layers of organic/inorganic materials. Furthermore, we show a new diagnostic monitoring application for use in healthcare, and a potential replacement of the Standard Kirby-Bauer Diffusion Antibiotic Resistance tests was developed and tested on both Escherichia coli and Xanthomonas alfalfae as pathogens with Oxytetracycline, Streptomycin and Kanamycin. We show that the reduction in area required for the minimum inhibitory concentration tests; allow for three times the number of tests to be performed within the same nutrient agar Petri dish, demonstrated both theoretically and experimentally resulting in correlations of R ≈ 0.96 and 0.985, respectively for both pathogens.
Virulence, Antibiotic Resistance, and Phylogenetic Relationships of Aeromonas spp. Carried by Migratory Birds in China
Authors: Liang Bing , Ji Xue , Jiang Bowen , Yuan Tingyu , Gerile Chao Lu Men , Zhu Lingwei , Wang Tiecheng , Li Yuanguo , Liu Jun , Guo Xuejun , Sun Yang
Published: December 2022

This study aimed to evaluate antimicrobial resistance, virulence, and the genetic diversity of Aeromonas isolated from migratory birds from Guangxi Province, Guangdong Province, Ningxia Hui Autonomous Region, Jiangxi Province, and Inner Mongolia in China. A total of 810 samples were collected, including fresh feces, cloacal swabs, and throat swabs. The collected samples were processed and subjected to bacteriological examination. The resistance to 21 antibiotics was evaluated. A phylogenetic tree was constructed using concatenated gltA-groL-gyrB-metG-PPSA-recA sequences. Eight putative virulence factors were identified by PCR and sequencing, and a biofilm formation assay was performed using a modified microtiter plate method. In total, 176 Aeromonas isolates were isolated including A. sobria, A. hydrophila, A. veronii, and A. caviae. All isolates showed variable resistance against all 16 tested antibiotic discs, and only one antibiotic had no reference standard. Six kinds of virulence gene markers were discovered, and the detection rates were 46.0% (hlyA), 76.1% (aerA), 52.3% (alt), 4.5% (ast), 54.0% (fla), and 64.2% (lip). These strains were able to form biofilms with distinct magnitudes; 102 were weakly adherent, 14 were moderately adherent, 60 were non-adherent, and none were strongly adherent. Our results suggest that migratory birds carry highly virulent and multidrug-resistant Aeromonas and spread them around the world through migration, which is a potential threat to public health.
Strain-Level Characterization of Legionella Environmental Isolates via MALDI-TOF-MS
Authors: Schwake David Otto , Sandrin Todd , Zhang Lin , Abbaszadegan Morteza
Published: December 2022

As a waterborne pathogen of increasing concern, techniques for cost-effective and rapid characterization of Legionella are vital. This study examines the development of a Matrix Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS) analysis methodology for this microbe. First, optimal sample preparation methods for the analysis of environmental Legionella isolates via MALDI-TOF-MS were determined. These methods were then implemented to perform strain-level characterization of environmental Legionella isolates from central Arizona. Results demonstrate that a MALDI-TOF-MS method involving BCYE agar-based culturing and protein extraction-based sample preparation yield high-quality mass spectra. Twenty-eight environmental Legionella isolates originating from two separate drinking water distribution systems were analyzed. Multiple species were detected, and strain-level characterization was achieved, with 12 unique strains distinguished. In addition, isolates of L. pneumophila, the most common species observed in the study, were correctly assigned to specific sampling sites. These results demonstrate the potential for this technique to be applied for sub-species characterization of Legionella with significant benefits over established methodologies.
Parallel Evolution to Elucidate the Contributions of PA0625 and parE to Ciprofloxacin Sensitivity in Pseudomonas aeruginosa
Authors: Liu Qi , Yin Liwen , Lv Chenjing , Bai Fang , Cheng Zhihui , Wu Weihui , Jin Yongxin
Published: December 2022

Pseudomonas aeruginosa is a ubiquitous pathogen that causes a wide range of acute and chronic infections. Ciprofloxacin, one of the first-line fluoroquinolone class antibiotics, is commonly used for the treatment of P. aeruginosa infections. However, ciprofloxacin-resistant P. aeruginosa is increasingly reported worldwide, making treatment difficult. To determine resistance-related mutations, we conducted an experimental evolution using a previously identified ciprofloxacin-resistant P. aeruginosa clinical isolate, CRP42. The evolved mutants could tolerate a 512-fold higher concentration of ciprofloxacin than CRP42. Genomic DNA reference mapping was performed, which revealed mutations in genes known to be associated with ciprofloxacin resistance as well as in those not previously linked to ciprofloxacin resistance, including the ParER586W substitution and PA0625 frameshift insertion. Simulation of the ParER586W substitution and PA0625 frameshift insertion by gene editing in CRP42 and the model strain PAO1 demonstrated that while the PA0625 mutation does contribute to resistance, mutation in the ParER586W does not contribute to resistance but rather affects tolerance against ciprofloxacin. These findings advance our understanding of ciprofloxacin resistance in P. aeruginosa.
Design Method and Application of Stope Structure Parameters in Deep Metal Mines Based on an Improved Stability Graph
Authors: Zhao Xingdong , Zhou Xin
Published: December 2022

Deep mining has become an inevitable trend of mining development. Previously conducted studies have established that reasonable stope structure parameters are the premise to ensure the safe and efficient production of deep mines. In order to ensure the safety of deep mining, in this paper, we systematically review the existing stope structure parameter design methods, and then put forward a deep stope structure design method based on the stability of mining rock mass. Based on rock mass quality classification, this method uses a critical span graph and an improved stability graph, and fully considers the influence of joint occurrence and mining stress on the stability of surrounding rock, to design the stope structural parameters. Taking into consideration the deterioration of the quality of deep rock mass, we collect mining data at home and abroad, improve the stability graph, and make it suitable for the design of stope structural parameters with different mining methods. The design process of stope structural parameters is expounded through field engineering cases, and it has specific guiding significance for the design of stope structural parameters in deep metal mines.
Early Diagenesis in the Lacustrine Ostracods from the Songliao Basin 91.35 Million Years Ago and Its Geological Implications
Authors: Liu Zhenwu , Liu Yuke , Du Xuejia , Lyu Dan , Wu Huaichun , Wang Huajian
Published: December 2022

Diagenesis is a double-edged sword of geochemical recordings. It makes us always doubt about the representativeness of many geochemical indicators, especially the isotope and mineral related. It also provides a window to explore the biogeochemical processes at the water–rock interface, which are related to the interactions between the hydrosphere, biosphere, and lithosphere. In this study, we identified microbial early diagenesis in lacustrine ostracods from the Songliao Basin 91.35 million years ago by using in situ mineralogical and carbon isotope analytical methods. Our results suggest multiple biological early-diagenesis processes and the formation of a ferric and methane transition zone (FMTZ) in the sulfate-poor pore water, which are conducive to the formation of dolomite and ankerite. These secondary carbonate minerals related to dissimilatory iron reduction and methanogenesis have heavier carbon isotopic compositions than the calcified ostracod shell in the water column and might bring interferences to the geochemical parameters of ostracods.
RETRACTED: Isolation and Characterization of Antibacterial Conglutinins from Lupine Seeds
Authors: Abdel-Shafi Seham , El-Nemr Mona , Enan Gamal , Osman Ali , Sitohy Basel , Sitohy Mahmoud
Published: December 2022

The main target of this work is to discover new protein fractions from natural resources with high antibacterial action. The 7S and 11S globulin fractions, as well as the basic subunit (BS), were isolated from lupine seeds (Lupinus termis), chemically characterized, and screened for antibacterial activity against seven pathogenic bacteria. SDS-PAGE revealed molecular weights ranging from 55 to 75 kDa for 7S globulin, 20–37 kD for 11S globulin, and 20 kD for the BS. 11S globulin and the BS migrated faster on Urea-PAGE toward the cathode compared to 7S globulin. FTIR and NMR showed different spectral patterns between the 7S and 11S globulins but similar ones between 11S globulin and the BS. The MICs of the BS were in the range of 0.05–2 μg/mL against Listeria monocytogenes, Klebsiella oxytoca, Proteus mirabilis, Staphylococcus aureus, Listeria ivanovii, Salmonella typhimurium, and Pseudomonas aeruginosa compared to higher values for 11S globulin. The BS surpassed 11S globulin in antibacterial action, while 7S globulin showed no effect. The MICs of 11S globulin and the BS represented only 5% and 2.5% of the specific antibiotic against L. monocytogenes, respectively. Scanning electron microscopy (SEM) demonstrated different signs of cellular deformation and decay in the protein-treated bacteria, probably due to interaction with the bacterial cell wall and membranes. 11S globulin and the BS can be nominated as effective food biopreservatives.
Capabilities of Single Cell ICP-MS for the Analysis of Cell Suspensions from Solid Tissues
Authors: Álvarez-Fernández García Roberto , Gutiérrez Romero Lucía , Bettmer Jörg , Montes-Bayón Maria
Published: December 2022

Single cell elemental (SC) analysis of isogenic cell cultures can be done using inductively coupled plasma (ICP-MS) detection. However, 2D cell cultures are just models to simplify the complexity of real tissue samples. Here, we show for the first time the capabilities of the technique (SC-ICP-MS) to analyze single cell suspensions of isolated cells from tissues. An optimized cocktail of proteolytic and collagenolytic enzymes was applied in a single preparation step with cellular yields up to 28% using 0.5 g of fresh rat spleen and liver, respectively. The retrieved cells revealed adequate morphology and stability to be examined by SC-ICP-MS. Quantitative elemental analysis of P, S, Cu, and Fe from disaggregated cells from rat spleen and liver tissues revealed levels of Fe of 7–16 fg/cell in the spleen and 8–12 fg/cell in the liver, while Cu was about 3–5 fg/cell in the spleen and 1.5–2.5 fg/cell in the liver. Evaluation of the transmembrane protein transferrin receptor 1 (TfR1) expression levels in disaggregated cells was also conducted by using a Nd-labelled antibody against this cell surface biomarker. Quantitative results showed significantly lower expression in the disaggregated cells than in the cell model HepG2, in agreement with the overexpression of this biomarker in tumor cells. In this proof of concept study, the tissue disaggregation protocol has shown to maintain the elemental intracellular content of cells as well as the presence of relevant antigens. This opens a completely new area of research for SC-ICP-MS in tissue samples as a complementary strategy with validation capabilities.
Gelatin-Coated Magnetic Nanowires for High-Sensitivity Optical Labels
Authors: Cuevas-Corona M. Charbel , Lopez-Romero J. Mauricio , Manzano-Ramírez Alejandro , Esparza Rodrigo , Zavala-Arce Rosa E. , Gimenez Alejandro J. , Luna-Bárcenas Gabriel
Published: December 2022

The encapsulation of magnetic nickel nanowires (NiNWs) with gelatin is proposed as an alternative for optical label detection. Magnetic nanowires can be detected at very low concentrations using light-scattering methods. This detection capacity could be helpful in applications such as transducers for molecular and biomolecular sensors; however, potential applications require the attachment of specific binding molecules to the nanowire structure. In the present study, a method is presented which is helpful in coating magnetic nanowires with gelatin, a material with the potential to handle specific decoration and functionalization of the nanowires; in the first case, silver nanoparticles (AgNPs) are efficiently used to decorate the nanowires. Furthermore, it is shown that the synthesized gelatin-coated particles have excellent detectability to the level of 140 pg/mL; this level of detection outperforms more complex techniques such as ICP-OES (~3 ng/mL for Ni) and magnetoresistance sensing (~10 ng/mL for magnetic nanoparticles).
Influence of Laboratory Synthesized Graphene Oxide on the Morphology and Properties of Cement Mortar
Authors: Ganesh Suganthiny , Thambiliyagodage Charitha , Perera S. V. T. Janaka , Rajapakse R. K. N. D.
Published: December 2022

The introduction of Graphene Oxide (GO), a nanomaterial, has shown considerable promise in improving the mechanical properties of cement composites. However, the reasons for this improvement are not yet fully understood and demand further research. This study aims to understand the effect of laboratory-produced GO, using Tour’s method, on the mechanical properties and morphology of cement mortar containing GO. The GO was characterized using Fourier-transform infrared spectroscopy, X-ray Photoelectron Spectroscopy (XRD), X-ray powder diffraction, and Raman spectroscopy alongside Scanning electron microscopy (SEM). This study adopted a cement mortar with GO percentages of 0.02, 0.025, 0.03, 0.035, and 0.04 with respect to the weight of the cement. The presence of GO in cement mortar increased the density and decreased the consistency and setting times. At the optimum of 0.03% GO viscous suspension, the mechanical properties such as the 28-day compressive strength, splitting tensile strength, and flexural strength were enhanced by 41%, 83%, and 43%, respectively. In addition, Brunauer–Emmett–Teller analysis indicates an increase in surface area and volume of micropores of GO cement mortar, resulting in a decreased volume of mesopores. The improvement in properties was due to increased nucleation sites, calcium silicate hydrate (CSH) density, and a decreased volume of mesopores.
Analysis of the Effect of Graphene, Metal, and Metal Oxide Transparent Electrodes on the Performance of Organic Optoelectronic Devices
Authors: Chen Ziqiang , Wang Zhenyu , Wang Jintao , Chen Shuming , Zhang Buyue , Li Ye , Yuan Long , Duan Yu
Published: December 2022

Transparent electrodes (TEs) are important components in organic optoelectronic devices. ITO is the mostly applied TE material, which is costly and inferior in mechanical performance, and could not satisfy the versatile need for the next generation of transparent optoelectronic devices. Recently, many new TE materials emerged to try to overcome the deficiency of ITO, including graphene, ultrathin metal, and oxide-metal-oxide structure. By finely control of the fabrication techniques, the main properties of conductivity, transmittance, and mechanical stability, have been studied in the literatures, and their applicability in the potential optoelectronic devices has been reported. Herein, in this work, we summarized the recent progress of the TE materials applied in optoelectronic devices by focusing on the fabrication, properties, such as Graphene, ultra-thin metal film, and metal oxide and performance. The advantages and insufficiencies of these materials as TEs have been summarized and the future development aspects have been pointed out to guide the design and fabrication TE materials in the next generation of transparent optoelectronic devices.
ME/CFS and Post-Exertional Malaise among Patients with Long COVID
Authors: Jason Leonard A. , Dorri Joseph A.
Published: December 2022

This study sought to ascertain the prevalence of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) among a sample of 465 patients with Long COVID. The participants completed three questionnaires: (1) a new questionnaire measuring both the frequency and severity of 38 common symptoms of COVID and Long COVID, (2) a validated short form questionnaire assessing ME/CFS, and (3) a validated questionnaire measuring post-exertional malaise. The population was predominantly white, female, and living in North America. The mean duration since the onset of COVID-19 symptoms was 70.5 weeks. Among the 465 participants, 58% met a ME/CFS case definition. Of respondents who reported that they had ME/CFS only 71% met criteria for ME/CFS and of those who did not report they had ME/CFS, 40% nevertheless did meet criteria for the disease: both over-diagnosis and under-diagnosis were evident on self-report. This study supports prior findings that ME/CFS occurs with high prevalence among those who have persistent COVID-19 symptoms.
Effects of a Web-Based Weight Loss Program on the Healthy Eating Index-NVS in Adults with Overweight or Obesity and the Association with Dietary, Anthropometric and Cardiometabolic Variables: A Randomized Controlled Clinical Trial
Authors: Kohl Jan , Brame Judith , Hauff Pascal , Wurst Ramona , Sehlbrede Matthias , Fichtner Urs Alexander , Armbruster Christoph , Tinsel Iris , Maiwald Phillip , Farin-Glattacker Erik , Fuchs Reinhard , Gollhofer Albert , König Daniel
Published: December 2022

This randomized, controlled clinical trial examined the impact of a web-based weight loss intervention on diet quality. Furthermore, it was investigated whether corresponding changes in diet quality were associated with changes in measures of cardiovascular risk profile. Individuals with a body mass index (BMI) of 27.5 to 34.9 kg/m2 and an age of 18 to 65 y were assigned to either an interactive and fully automated web-based weight loss program focusing on dietary energy density (intervention) or a non-interactive web-based weight loss program (control). Examinations were performed at baseline (t0), after the 12-week web-based intervention (t1), and after an additional 6 (t2) and 12 months (t3). Based on a dietary record, the Healthy Eating Index-NVS (HEI-NVS) was calculated and analyzed using a robust linear mixed model. In addition, bootstrapped correlations were performed independently of study group to examine associations between change in HEI-NVS and change in dietary, anthropometric, and cardiometabolic variables. A total of n = 153 participants with a mean BMI of 30.71 kg/m2 (SD 2.13) and an average age of 48.92 y (SD 11.17) were included in the study. HEI-NVS improved significantly in the intervention group from baseline (t0) to t2 (p = 0.003) and to t3 (p = 0.037), whereby the course was significantly different up to t2 (p = 0.013) and not significantly different up to t3 (p = 0.054) compared to the control group. Independent of study group, there was a significant negative association between change in HEI-NVS and dietary energy density. A higher total score in HEI-NVS did not correlate with improvements in cardiovascular risk profile. The interactive and fully automated web-based weight loss program improved diet quality. Independent of study group, changes in HEI-NVS correlated with changes in energy density, but there was no association between improvements in HEI-NVS and improvements in cardiovascular risk profile.
The Association of Perceived Vulnerability to Disease with Cognitive Restraint and Compensatory Behaviors
Authors: Hoover Lindzey V. , Ackerman Joshua M. , Cummings Jenna R. , Gearhardt Ashley N.
Published: December 2022

Individual differences exist in perceived vulnerability to disease (PVD). PVD is associated with negative responses (e.g., disgust) towards individuals with obesity and heightened sensitivity regarding personal appearance. Through increasing fear of fat (FOF), PVD may be associated with cognitive restraint and compensatory behaviors. We utilized an adult sample (n = 247; 53.3% male sex assigned at birth) recruited through Amazon’s MTurk prior to the COVID-19 pandemic to investigate associations between PVD, cognitive restraint and compensatory behaviors. Participants completed the Perceived Vulnerability to Disease Scale, Eating Disorder Diagnostic Scale, Dutch Eating Behaviors Questionnaire, and Goldfarb’s Fear of Fat Scale. Mediation analyses were used to test our hypotheses. Perceived infectability (PVD-Infection) was associated with cognitive restraint and compensatory behaviors through increased FOF. Perceived germ aversion (PVD–Germ) was associated with cognitive restraint, but FOF did not mediate this association. Sex-stratified analyses revealed no significant sex differences. PVD may be an overlooked factor associated with cognitive restraint and compensatory behaviors in males and females. FOF was an important mediating factor in these associations. Increased engagement in cognitive restraint and compensatory behaviors may reflect attempts to reduce FOF. Future longitudinal research should explore whether PVD is a risk factor for cognitive restraint and compensatory behaviors.
Effect of Lean Red Meat from Beef (Pirenaica Breed) Versus Lean White Meat Consumption on Diet Quality: A Randomized-Controlled Crossover Study in Healthy Young Adults
Authors: Miguel-Berges Maria Luisa , Fajó-Pascual Marta , Moreno Luis A. , Campo Marimar , Guerrero Ana , Olleta Jose Luis , Santolaria Blasco Pilar , Santaliestra-Pasías Alba M.
Published: December 2022

A randomized crossover study was carried out in three University accommodation halls. Participants consumed either beef (Pirenaica breed) (PB) or conventional chicken (CC) three times per week for an 8-week periods with their usual diet, each one separated by a 5-week wash out period. Dietary variables were recollected by the Food Frequency Questionnaire (FFQ), and the Diet Quality Index (DQI) was calculated. Forty-seven healthy adults were included (19.9 ± 1.75 years). The inclusion of both types of diets did not modify the components of the DQI, such as the diversity, equilibrium, adequacy and excess. However, when only the first period was analyzed, a significant decrease in the consumption of fruits and vegetables was observed in those participants who received the PB diet (intervention group). The CC diet (control group) significantly reduced the consumption of fish and eggs, total DQI, and DQI quality component. The expected effect was observed in the significant increment of consumption of red meat after the intervention period.
Garcinone E Mitigates Oxidative Inflammatory Response and Protects against Experimental Autoimmune Hepatitis via Modulation of Nrf2/HO-1, NF-κB and TNF-α/JNK Axis
Authors: Mohamed Gamal A. , Ibrahim Sabrin R. M. , Hareeri Rawan H. , Binmahfouz Lenah S. , Bagher Amina M. , Abdallah Hossam M. , Elsaed Wael M. , El-Agamy Dina S.
Published: December 2022

Garcinia mangostana L. (Clusiaceae), a popular tropical fruit for its juiciness and sweetness, is an opulent fountain of prenylated and oxygenated xanthones with a vast array of bio-activities. Garcinone E (GE), a xanthone derivative reported from G. mangostana, possesses cytotoxic and aromatase inhibitory activities. The present research endeavors to investigate the hepato-protection efficaciousness of GE on concanavalin-A (Con-A)-instigated hepatitis. Results showed that GE pretreating noticeably diminishes both the serum indices (transaminases, ALP, LDH, and γ-GT) and histopathological lesions of the liver. It counteracted neutrophil and CD4+ infiltration into the liver. GE furthered the Nrf2 genetic expression and its antioxidants’ cascade, which resulted in amelioration of Con-A-caused oxidative stress (OS), lipid per-oxidative markers (4-HNE, MDA, PC) reduction, and intensified antioxidants (TAC, SOD, GSH) in the hepatic tissue. Additionally, GE prohibited NF-ĸB (nuclear factor kappa-B) activation and lessened the genetics and levels of downstream cytokines (IL1β and IL6). Moreover, the TNF-α/JNK axis was repressed in GE-treated mice, which was accompanied by attenuation of Con-A-induced apoptosis. These findings demonstrated the protective potential of GE in Con-A-induced hepatitis which may be associated with Nrf2/HO-1 signaling activation and OS suppression, as well as modulation of the NF-κB and TNF-α/JNK/apoptosis signaling pathway. These results suggest the potential use of GE as a novel hepato-protective agent against autoimmune hepatitis.
Exercise Promotes Bone Marrow Microenvironment by Inhibiting Adipsin in Diet-Induced Male Obese Mice
Authors: Shi Zunhan , Wang Lihui , Luan Jinwen , Yin Liqin , Ji Xiaohui , Zhang Wenqian , Xu Bingxiang , Chen Linshan , He Ying , Wang Ru , Liu Longhua
Published: December 2022

Obesity is a growing global epidemic linked to many diseases, including diabetes, cardiovascular diseases, and musculoskeletal disorders. Exercise can improve bone density and decrease excess bone marrow adipose tissue (BMAT) in obese individuals. However, the mechanism of exercise regulating bone marrow microenvironment remains unclear. This study examines how exercise induces bone marrow remodeling in diet-induced obesity. We employed unbiased RNA-Seq to investigate the effect of exercise on the bone marrow of diet-induced obese male mice. Bone mesenchymal stem cells (BMSCs) were isolated to explore the regulatory effects of exercise in vitro. Our data demonstrated that exercise could slow down the progression of obesity and improve trabecular bone density. RNA-seq data revealed that exercise inhibited secreted phosphoprotein 1 (Spp1), which was shown to mediate bone resorption through mechanosensing mechanisms. Interactome analysis of Spp1 using the HINT database showed that Spp1 interacted with the adipokine adipsin. Moreover, exercise decreased BMAT, which induced osteoclast differentiation and promoted bone loss. Our study reveals that exercise improves the bone marrow microenvironment by at least partially inhibiting the adipsin–Spp1 signaling pathway so as to inhibit the alternative complement system from activating osteoclasts in diet-induced obese mice.
What Promotes Acute Kidney Injury in Patients with Myocardial Infarction and Multivessel Coronary Artery Disease—Contrast Media, Hydration Status or Something Else?
Authors: Maksimczuk Joanna , Galas Agata , Krzesiński Paweł
Published: December 2022

Multivessel coronary artery disease (MVCAD) is found in approximately 50% of patients with acute myocardial infarction (AMI) undergoing percutaneous coronary intervention (PCI). Although we have data showing the benefits of revascularization of significant non-culprit coronary lesions in patients with AMI, the optimal timing of angioplasty remains unclear. The most common reason for postponing subsequent percutaneous treatment is the fear of contrast-induced acute kidney injury (CI-AKI). Acute kidney injury (AKI) is common in patients with AMI undergoing PCI, and its etiology appears to be complex and incompletely understood. In this review, we discuss the definition, pathophysiology and risk factors of AKI in patients with AMI undergoing PCI. We present the impact of AKI on the course of hospitalization and distant prognosis of patients with AMI. Special attention was paid to the phenomenon of AKI in patients undergoing multivessel revascularization. We analyze the correlation between increased exposure to contrast medium (CM) and the risk of AKI in patients with AMI to provide information useful in the decision-making process about the optimal timing of revascularization of non-culprit lesions. In addition, we present diagnostic tools in the form of new biomarkers of AKI and discuss ways to prevent and mitigate the course of AKI.