bluuebunny/crossref_metadata_2025 huggingface dataset:
The Gene FvTST1 From Strawberry Modulates Endogenous Sugars Enhancing Plant Growth and Fruit Ripening
Authors: Rashid Arif , Ruan Haixiang , Wang Yunsheng
Published: January 2022

Sugar is an important carbon source and contributes significantly to the improvement of plant growth and fruit flavor quality. Sugar transport through the tonoplast is important for intracellular homeostasis and metabolic balance in plant cells. There are four tonoplast sugar transporters (FvTST1-4) in strawberry genome. The qRT-PCR results indicated that FvTST1 has a differential expression pattern in different tissues and developmental stages, and exhibited highest expression level in mature fruits. The yeast complementation assay showed that FvTST1 can mediate the uptake of different sugars, such as fructose, glucose, sucrose, and mannose. Subcellular localization analyses revealed that FvTST1 was mainly targeted to the tonoplast. Transient expression of FvTST1 in strawberry fruits enhanced both fruit ripening and sugar accumulation. Furthermore, FvTST1-transformed tomato plants exhibited higher sucrose and auxin content, enhanced seed germination and vegetative growth, higher photosynthetic rate, early flowering, and bore fruit; fructose and glucose levels were higher in transgenic fruits than those in the control. Transcriptomic analysis indicated that the auxin signaling pathway was highly enriched pathway in up-regulated Gene-ontology terms. In transgenic plants, genes encoding transcription factors, such as phytochrome-interacting factors PIF1, -3, and -4, as well as their potential target genes, were also induced. Collectively, the results show that FvTST1 enhances plant growth and fruit ripening by modulating endogenous sugars, and highlight the biological significance of this gene for future breeding purposes.
Research Advances in Multi-Omics on the Traditional Chinese Herb Dendrobium officinale
Authors: Wang Yue , Tong Yan , Adejobi Oluwaniyi Isaiah , Wang Yuhua , Liu Aizhong
Published: January 2022

Dendrobium officinale Kimura et Migo is an important epiphytic plant, belonging to the Orchidaceae family. There are various bioactive components in D. officinale plants, mainly including polysaccharides, alkaloids, and phenolic compounds. These compounds have been demonstrated to possess multiple functions, such as anti-oxidation, immune regulation, and anti-cancer. Due to serious shortages of wild resources, deterioration of cultivated germplasm and the unstable quality of D. officinale, the study has been focused on the biosynthetic pathway and regulation mechanisms of bioactive compounds. In recent years, with rapid developments in detection technologies and analysis tools, omics research including genomics, transcriptomics, proteomics and metabolomics have all been widely applied in various medicinal plants, including D. officinale. Many important advances have been achieved in D. officinale research, such as chromosome-level reference genome assembly and the identification of key genes involved in the biosynthesis of active components. In this review, we summarize the latest research advances in D. officinale based on multiple omics studies. At the same time, we discuss limitations of the current research. Finally, we put forward prospective topics in need of further study on D. officinale.
Children Use Non-referential Gestures in Narrative Speech to Mark Discourse Elements Which Update Common Ground
Authors: Rohrer Patrick Louis , Florit-Pons Júlia , Vilà-Giménez Ingrid , Prieto Pilar
Published: January 2022

While recent studies have claimed that non-referential gestures (i.e., gestures that do not visually represent any semantic content in speech) are used to mark discourse-new and/or -accessible referents and focused information in adult speech, to our knowledge, no prior investigation has studied the relationship between information structure (IS) and gesture referentiality in children’s narrative speech from a developmental perspective. A longitudinal database consisting of 332 narratives performed by 83 children at two different time points in development was coded for IS and gesture referentiality (i.e., referential and non-referential gestures). Results revealed that at both time points, both referential and non-referential gestures were produced more with information that moves discourse forward (i.e., focus) and predication (i.e., comment) rather than topical or background information. Further, at 7–9 years of age, children tended to use more non-referential gestures to mark focus and comment constituents than referential gestures. In terms of the marking of the newness of discourse referents, non-referential gestures already seem to play a key role at 5–6 years old, whereas referential gestures did not show any patterns. This relationship was even stronger at 7–9 years old. All in all, our findings offer supporting evidence that in contrast with referential gestures, non-referential gestures have been found to play a key role in marking IS, and that the development of this relationship solidifies at a period in development that coincides with a spurt in non-referential gesture production.
Role of Peer Coaching in Transmitting the Benefits of Leader Coaching
Authors: Dong Yanan , Dong Huijuan , Yuan Yuan , Jiang Jing
Published: January 2022

Drawing on social information processing theory, the present study examines how and when leader coaching can be beneficial for team performance. Based on a sample of 58 teams from a sanitary product company in China, we found that peer coaching served as a mediator linking leader coaching and team performance. Moreover, the team individualistic/collectivism value moderated the first-stage relationship that the relationship between leader coaching and peer coaching was more positive when the team individualism value was low, but not significant when the team individualism value was high; while team task interdependence moderated the second-stage relationship that the relationship between peer coaching and team performance was more positive when the team task interdependence was high, but not significant when it was low. The findings enrich our understandings of the effectiveness of leader coaching behavior by uncovering the theoretical mechanism and boundary conditions. The study also provides important implications for coaching practice in organizations.
Associations of Wellbeing Levels, Changes, and Within-Person Variability With Late-Life All-Cause Mortality Across 12 Years: Contrasting Hedonic vs. Eudaimonic Wellbeing Among Very Old Adults
Authors: Schilling Oliver Karl , Wettstein Markus , Wahl Hans-Werner
Published: January 2022

Advanced old age has been characterized as a biologically highly vulnerable life phase. Biological, morbidity-, and cognitive impairment-related factors play an important role as mortality predictors among very old adults. However, it is largely unknown whether previous findings confirming the role of different wellbeing domains for mortality translate to survival among the oldest-old individuals. Moreover, the distinction established in the wellbeing literature between hedonic and eudaimonic wellbeing as well as the consideration of within-person variability of potentially relevant mortality predictors has not sufficiently been addressed in prior mortality research. In this study, we examined a broad set of hedonic and eudaimonic wellbeing indicators, including their levels, their changes, as well as their within-person variability, as predictors of all-cause mortality in a sample of very old individuals. We used data from the LateLine study, a 7-year longitudinal study based on a sample ofn= 124 individuals who were living alone and who were aged 87–97 years (M= 90.6,SD= 2.9) at baseline. Study participants provided up to 16 measurement occasions (mean number of measurement occasions per individual = 5.50,SD= 4.79) between 2009 and 2016. Dates of death were available for 118 individuals (95.2%) who had deceased between 2009 and 2021. We ran longitudinal multilevel structural equation models and specified between-person level differences, within-person long-term linear change trends, as well as the “detrended” within-person variability in three indicators of hedonic (i.e., life satisfaction and positive and negative affect) and four indicators of eudaimonic wellbeing (i.e., purpose in life, autonomy, environmental mastery, and self-acceptance) as all-cause mortality predictors. Controlling for age, gender, education, and physical condition and testing our sets of hedonic and eudaimonic indictors separately in terms of their mortality impact, solely one eudaimonic wellbeing indicator, namely, autonomy, showed significant effects on survival. Surprisingly, autonomy appeared “paradoxically” related with mortality, with high individual levels and intraindividual highly stable perceptions of autonomy being associated with a shorter residual lifetime. Thus, it seems plausible that accepting dependency and changing perceptions of autonomy over time in accordance with objectively remaining capabilities might become adaptive for survival in very old age.
An Ecological Approach to Evaluating Collaborative Practice in NSF Sponsored Partnership Projects: The SPARC Model
Authors: Burr Erin M. , Kelly Kimberle A. , Murphrey Theresa P. , Koswatta Taniya J.
Published: January 2022

From co-authored publications to sponsored projects involving multiple partner institutions, collaborative practice is an expected part of work in the academy. As evaluators of a National Science Foundation (NSF) Alliances for Graduate Education and the Professoriate (AGEP) grant awarded to four university partners in a large southern state, the authors recognized the increasing value of collaborative practice in the design, implementation, evaluation, and dissemination of findings in the partnership over time. When planning a program among partnering institutions, stakeholders may underestimate the need for, and value of, collaborative practice in facilitating partnership functioning. This method paper outlines an evaluative model to increase the use of collaborative practice in funded academic partnership programs. The model highlights collaborative practice across multiple stakeholder groups in the academic ecology: Sponsors of funded programs (S), Program partners and participants (P), Assessment and evaluation professionals (A), academic researchers (R), and the national and global Community (C). The SPARC model emphasizes evidence-based benefits of collaborative practice across multiple outcome domains. Tools and frameworks for evaluating collaborative practice take a view of optimizing partnership operational performance in achieving stated goals. Collaborative practice can also be an integral element of program activities that support the academic success and scholarly productivity, psychosocial adjustment, and physical and psychological well-being of stakeholders participating in the program. Given the goal of our alliance to promote diversification of the professoriate, the model highlights the use of collaborative practice in supporting stakeholders from groups historically underrepresented in STEM fields across these outcome domains. Using data from a mixed-methods program evaluation of our AGEP alliance over 4 years, the authors provide concrete examples of collaborative practice and their measurement. Results discuss important themes regarding collaborative practice that emerged in each stakeholder group. Authors operationalize the SPARC model with a checklist to assist program stakeholders in designing for and assessing collaborative practice in support of project goals in funded academic partnership projects, emphasizing the contributions of collaborative practice in promoting diversification of the professoriate.
The Specificity and Reliability of Conflict Adaptation: A Mouse-Tracking Study
Authors: Grundy John G.
Published: January 2022

Researchers have recently begun to question the specificity and reliability of conflict adaptation effects, also known as sequential congruency effects (SCEs), a highly cited effect in cognitive psychology. Some have even used the lack of reliability across tasks (e.g., Flanker, and Stroop) to argue against models of cognitive control that have dominated the field for decades. The present study tested the possibility that domain-general processes across tasks might appear on more sensitive mouse-tracking metrics rather than overall reaction times. The relationship between SCE effects on the Stroop and Flanker tasks were examined for the first time using a mouse-tracking paradigm. Three main findings emerged: (1) Robust SCEs were observed for both the Stroop and Flanker tasks at the group level, (2) Within-task split-half reliabilities for the SCE across dependent variables were weak at best and non-existent in many cases, and (3) SCEs for the Flanker and Stroop tasks did not correlate with each other for overall reaction times, but did show significant correlations between tasks on more dynamic measures that captured processes before response execution. These findings contribute to the literature by highlighting how mouse-tracking may be a fruitful avenue by which future studies can examine the specificity and reliability of conflict adaptation and tease apart different theoretical models producing the effects.
Interpersonal Affective Touch in a Virtual World: Feeling the Social Presence of Others to Overcome Loneliness
Authors: Della Longa Letizia , Valori Irene , Farroni Teresa
Published: January 2022

Humans are by nature social beings tuned to communicate and interact from the very beginning of their lives. The sense of touch represents the most direct and intimate channel of communication and a powerful means of connection between the self and the others. In our digital age, the development and diffusion of internet-based technologies and virtual environments offer new opportunities of communication overcoming physical distance. It however, happens that social interactions are often mediated, and the tactile aspects of communication are overlooked, thus diminishing the feeling of social presence, which may contribute to an increased sense of social disconnection and loneliness. The current manuscript aims to review the extant literature about the socio-affective dimension of touch and current advancements in interactive virtual environments in order to provide a new perspective on multisensory virtual communication. Specifically, we suggest that interpersonal affective touch might critically impact virtual social exchanges, promoting a sense of co-presence and social connection between individuals, possibly overcoming feelings of sensory loneliness. This topic of investigation will be of crucial relevance from a theoretical perspective aiming to understand how we integrate multisensory signals in processing and making sense of interpersonal exchanges, this is important in both typical and atypical populations. Moreover, it will pave the way to promising applications by exploring the possibility to use technical innovations to communicate more interactively in the case of people who suffer from social isolation and disconnection from others.
Measuring What Counts in Life: The Development and Initial Validation of the Fulfilled Life Scale (FLS)
Authors: Baumann Doris , Ruch Willibald
Published: January 2022

In a recent work, we introduced a theoretical model for fulfillment in life that covers cognitive and affective components and distinguishes different time frames. The present study evaluates this model and describes the construction of the Fulfilled Life Scale (FLS) to assess fulfillment regarding the whole lived life retrospectively. We investigated the scale in two samples (Sample 1: N = 282 adults aged 50–93 years; Sample 2: N = 406 adults aged 40–85 years). The model of the cognitive component combines three sources of fulfillment (self, life, impact/legacy) with three criteria (wholeness, fit, value), yielding nine facets. Employing hierarchical factor analysis, we inspected all solutions between one and nine. We identified three optimal factors, which we labeled unfolded self and life, the worthwhile life, and positive impact and legacy. Next, we selected marker items and replicated the factor structure in Sample 2. The three scales were positively intercorrelated and showed good internal consistency in both samples. For the affective component, exploratory and confirmatory factor analyses established a one-factor structure in both samples, and high internal consistency was obtained. Across a range of related constructs, we demonstrated construct and criterion validity. Notably, cognitive and affective fulfillment incrementally predicted a global rating of a fulfilled life and mental well-being, even after controlling for subjective and eudaimonic well-being. Overall, the study proves that the FLS is necessary to capture people’s experience of a fulfilled life, which could not be assessed sufficiently with previous well-being measures. Both cognitive and affective fulfillment were able to predict additional variance in mental well-being. Moreover, the study reveals psychometric support for the FLS and presents the first evidence on its validity. Lastly, applications in research and practice are discussed, especially in the context of living and aging well in the second half of life.
URMC Universal Depression Screening Initiative: Patient Reported Outcome Assessments to Promote a Person-Centered Biopsychosocial Population Health Management Strategy
Authors: Van Orden Kimberly A. , Lutz Julie , Conner Kenneth R. , Silva Caroline , Hasselberg Michael J. , Fear Kathleen , Leadley Allison W. , Wittink Marsha N. , Baumhauer Judith F.
Published: January 2022

Background: Patient-reported outcomes (PROs) can promote person-centered biopsychosocial health care by measuring outcomes that matter to patients, including functioning and well-being. Data support feasibility and acceptability of PRO administration as part of routine clinical care, but less is known about its effects on population health, including detection of unmet healthcare needs. Our objectives were to examine differences in rates of clinically significant depression across sociodemographic groups and clinical settings from universal depression screens in a large health system, estimate the number of patients with untreated depression detected by screenings, and examine associations between biopsychosocial PROs—physical, psychological, and social health.

Methods: We analyzed data from over 200,000 adult patients who completed depression screens—either PROMIS (Patient Reported Outcomes Measurement Information System) or PHQ-2/9—as part of routine outpatient care.

Results: Depression screens were positive in 14.2% of the sample, with more positive screens among younger vs. older adults, women vs. men, non-White vs. White, and Hispanics vs. non-Hispanics. These same sociodemographic indicators, as well as completing screening in primary care (vs. specialty care) were also associated with greater likelihood of detected depression in the medical record.

Discussion: Universal screening for depression symptoms throughout a large health system appears acceptable and has the potential to detect depression in diverse patient populations outside of behavioral health. Expanded delivery of PROs to include physical and social health as well as depression should be explored to develop a clinically-relevant model for addressing patients' biopsychosocial needs in an integrated fashion across the health system.
Multiple Substance Use Disorders and Self-Reported Cognitive Function in U.S. Adults: Associations and Sex-Differences in a Nationally Representative Sample
Authors: Bourgault Zoe , Rubin-Kahana Dafna Sara , Hassan Ahmed Nabeel , Sanches Marcos , Le Foll Bernard
Published: January 2022

Polysubstance use is a growing public health concern that has been associated with poor clinical outcomes. Compared to single-drug users, this population suffers greater deficits in cognitive function, which hinder treatment success and recovery. Despite its high prevalence and poor prognosis, epidemiological research on polysubstance use and accompanying cognitive profile is lacking. We investigated associations between numbers of past-year co-occurring substance use disorders (SUDs) and self-reported cognitive function using data from the National Epidemiologic Survey for Alcohol and Related Conditions III (NESARC-III). Regression analyses revealed a significant negative association between cognitive scores and numbers of past-year SUDs, which was moderated by sex. After adjusting for confounding variables, greater numbers of SUDs were associated with declining self-reported cognitive function, and this relationship was stronger among females. Our findings expand on current literature on cognitive impairments among polysubstance users and provide a novel, nuanced description of this relationship among the general population. We highlight the need for targeted and individualized treatment approaches in order to improve outcomes in this population.
The Rise and Fall of Dopamine: A Two-Stage Model of the Development and Entrenchment of Anorexia Nervosa
Authors: Beeler Jeff A. , Burghardt Nesha S.
Published: January 2022

Dopamine has long been implicated as a critical neural substrate mediating anorexia nervosa (AN). Despite nearly 50 years of research, the putative direction of change in dopamine function remains unclear and no consensus on the mechanistic role of dopamine in AN has been achieved. We hypothesize two stages in AN– corresponding to initial development and entrenchment– characterized by opposite changes in dopamine. First, caloric restriction, particularly when combined with exercise, triggers an escalating spiral of increasing dopamine that facilitates the behavioral plasticity necessary to establish and reinforce weight-loss behaviors. Second, chronic self-starvation reverses this escalation to reduce or impair dopamine which, in turn, confers behavioral inflexibility and entrenchment of now established AN behaviors. This pattern of enhanced, followed by impaired dopamine might be a common path to many behavioral disorders characterized by reinforcement learning and subsequent behavioral inflexibility. If correct, our hypothesis has significant clinical and research implications for AN and other disorders, such as addiction and obesity.
Validity and Acceptability of Wearable Devices for Monitoring Step-Count and Activity Minutes Among People With Multiple Sclerosis
Authors: Lavelle Grace , Norris Meriel , Flemming Julie , Harper Jamie , Bradley Joan , Johnston Helen , Fortune Jennifer , Stennett Andrea , Kilbride Cherry , Ryan Jennifer M.
Published: January 2022

Multiple wearable devices that purport to measure physical activity are widely available to consumers. While they may support increases in physical activity among people with multiple sclerosis (MS) by providing feedback on their performance, there is little information about the validity and acceptability of these devices. Providing devices that are perceived as inaccurate and difficult to use may have negative consequences for people with MS, rather than supporting participation in physical activity. The aim of this study was, therefore, to assess the validity and acceptability of commercially available devices for monitoring step-count and activity time among people with MS. Nineteen ambulatory adults with MS [mean (SD) age 52.1 (11.9) years] participated in the study. Step-count was assessed using five commercially available devices (Fitbit Alta, Fitbit Zip, Garmin Vivofit 4, Yamax Digi Walker SW200, and Letscom monitor) and an activPAL3μ while completing nine everyday activities. Step-count was also manually counted. Time in light activity, moderate-to-vigorous activity, and total activity were measured during activities using an Actigraph GT3X accelerometer. Of the 19 participants who completed the validity study, fifteen of these people also wore the five commercially available devices for three consecutive days each, and participated in a semi-structured interview regarding their perception of the acceptability of the monitors. Mean percentage error for step-count ranged from 12.1% for the Yamax SW200 to −112.3% for the Letscom. Mean step-count as manually determined differed to mean step-count measured by the Fitbit Alta (p = 0.002), Garmin vivofit 4 (p < 0.001), Letscom (p < 0.001) and the research standard device, the activPAL3μ (p < 0.001). However, 95% limits of agreement were smallest for the activPAL3μ and largest for the Fitbit Alta. Median percentage error for activity minutes was 52.9% for the Letscom and 100% for the Garmin Vivofit 4 and Fitbit Alta compared to minutes in total activity. Three inductive themes were generated from participant accounts: Interaction with device; The way the device looks and feels; Functionality. In conclusion, commercially available devices demonstrated poor criterion validity when measuring step-count and activity time in people with MS. This negatively affected the acceptability of devices, with perceived inaccuracies causing distrust and frustration. Additional considerations when designing devices for people with MS include an appropriately sized and lit display and ease of attaching and charging devices.
Never Ask for a Lighter Rain but a Stronger Umbrella
Authors: Pande Saket , Haeffner Melissa , Blöschl Günter , Alam Mohammad Faiz , Castro Cyndi , Di Baldassarre Giuliano , Frick-Trzebitzky Fanny , Hogeboom Rick , Kreibich Heidi , Mukherjee Jenia , Mukherji Aditi , Nardi Fernando , Nüsser Marcus , Tian Fuqiang , van Oel Pieter , Sivapalan Murugesu
Published: January 2022

In a recent editorial in the journal Nature Sustainability, the editors raised the concern that journal submissions on water studies appear too similar. The gist of the editorial: “too many publications and not enough ideas.” In this response, we contest this notion, and point to the numerous new ideas that result from taking a broader view of the water science field. Drawing inspiration from a recently hosted conference geared at transcending traditional disciplinary silos and forging new paradigms for water research, we are, in fact, enthusiastic and optimistic about the ways scientists are investigating political, economic, historical, and cultural intersections toward more just and sustainable human-water relations and ways of knowing.
On (Dis)Connections and Transformations: The Role of the Agricultural Innovation System in the Adoption of Improved Forages in Colombia
Authors: Enciso Karen , Triana Natalia , Díaz Manuel , Burkart Stefan
Published: January 2022

Feeding improvement strategies are key in increasing cattle productivity and reducing its environmental footprint. Nevertheless, Colombian tropical cattle systems still feature serious deficiencies in both forage quality and availability. As a result of past and on-going forage Research and Development (R&D) processes, institutions have released 23 grass and legume cultivars of superior characteristics in terms of forage quality, supply, or adaptation to different soil and climate conditions, while providing numerous environmental benefits. However, low levels of adoption are observed: although R&D processes are a necessary condition for adoption, they are still not sufficient to guarantee agricultural technification in Colombia. The ultimate success occurs only when end-users make effective use of a technology–a link constantly interrupted. Agricultural innovation requires complex processes of interaction in which knowledge is shared amongst organizations involved in the Agricultural Innovation System (AIS), namely: suitable links, attitudes, practices, governance structures, and policies. The objective of this study is to identify limitations and opportunities in R&D, adoption, and diffusion of forage technologies in Colombia from an AIS perspective. Particularly, we present a study case pertaining to research institutions only, to (a) map the involved actors and describe their roles and links, and (b) identify the events that marked the evolution of the AIS and the course of forage R&D in its research-related components. We applied a qualitative methodology based on focus group discussions, in-depth interviews, literature review, and historical analysis. Results show that the complex nature of institutions and the interactions between them determine the historical transformation of diffusion of forage technologies. The lack of connection between institutions and the weak intensity of the relationships, prevent the convergence of interests and objectives, leading to vicious cycles that hamper technology adoption. Insufficient synchronization between institutions of different nature (and even between those that share similar objectives) results in efficiency losses due to an unnecessary repetition of activities and processes. We provide recommendations for policy- and decision-makers that will help in both a restructuration of the AIS and a better allocation of funds for R&D, and thus support the development of more effective pathways for forage adoption and scaling.
Impact of Crop Diversification on Household Food and Nutrition Security in Southern and Central Mali
Authors: Douyon Adama , Worou Omonlola Nadine , Diama Agathe , Badolo Felix , Denou Richard Kibarou , Touré Sidi , Sidibé Amadou , Nebie Baloua , Tabo Ramadjita
Published: January 2022

Many African countries, including Mali, depend on the production of a single or a limited range of crops for national food security. In Mali, this heavy reliance on a range of basic commodities or staple crops, or even just one, exacerbates multiple risks to agricultural production, rural livelihoods, and nutrition. With this in mind, the smart food campaign was initiated to strengthen the resilience and nutritional situation of households and peasant communities where the diet is mainly cereal-based and remains very undiversified and poor in essential micronutrients. As part of the campaign, our study aims to analyze the impact of agricultural diversification on food consumption and household nutritional security. The analysis uses survey data from 332 individuals randomly selected. Multinomial logistic regression and the Simpson diversity index were used to determine the index and estimate the determinants of crop diversification. The consumption score index weighted by consumption frequency and anthropometric indices (for children) were used to assess the nutritional status of households. The results show four types of strategies of diversification: 7.55% are cereals only, 5.66% combine millet–sorghum–groundnut, 41.51% combine millet–sorghum–groundnut–cowpea, and 45.28% combine millet–sorghum–groundnut–cowpea–maize. The estimation of the regression model shows that socioeconomic factors have a positive influence. With a consumption score index of 34 in the villages and 40.5 in Bamako, based on eight food groups, we find that the quality of food is insufficient in rural areas, but it is acceptable in the urban center of Bamako. Analysis of the nutritional status of children aged 6–48 months reveals that 30% of the surveyed population is in a situation of nutritional insecurity (all forms combined). To help improve crop diversification and the nutritional quality of foods, we suggest, among other things, subsidies and public spending to facilitate access to inputs that allow the acquisition of a wider range of inputs and services, intensification of nutrition awareness, and education programs to maximize the incentive to consume nutritious foods from self-production and market purchases. Finally, we propose to facilitate access to technologies promoting food diversification and improving food and nutritional security, particularly in rural areas.
Comparison of Intra-CL Injection and Peripheral Application of Prostaglandin F2α Analog on Luteal Blood Flow and Secretory Function of the Bovine Corpus Luteum
Authors: Jonczyk Agnieszka W. , Piotrowska-Tomala Katarzyna K. , Skarzynski Dariusz J.
Published: January 2022

We investigated the effects of different doses of dinoprost injected directly into the bovine corpus luteum (CL) on (i) concentrations of progesterone (P4) and oxytocin (OT) in peripheral blood and (ii) mRNA levels of steroidogenic acute regulatory protein (STAR), cytochrome P450 family 11 subfamily A member 1 (P450scc), hydroxy-delta-5-steroid dehydrogenase, 3 β- and steroid delta-isomerase 1 (HSD3B), and receptor-interacting protein kinases 1 and 3 (RIPK1, RIPK3) in CL tissue. Moreover, we examined the effects of dinoprost, injected intra-CL or administered intramuscularly (IM), on CL secretory function and on indicators of CL vascular network status: luteal tissue area (LTA), CL blood flow (CLBF), and the CLBF:LTA ratio (Adj. CLBF), in cows at the early and mid-luteal phases. In the Experiment 1, cows (day 10 of the cycle) were allocated to (i) an intra-CL injection of saline (control; n = 3); (ii) an intra-CL injection of dinoprost (1.25 mg; 2.5 mg, or 5 mg; n = 3 for each dose); (iii) an IM administration of saline (control; n = 3); or (iv) an IM administration of dinoprost (25 mg; positive control; n = 3). Concentrations of OT and P4 were measured in plasma samples. The mRNA expression of steroidogenesis- or necroptosis-related factors was determined in CL tissue 4 h after treatments. In Experiment 2, cows on day 4 (n = 12) or day 10 (n = 12) were allocated to (i) an intra-CL injection of dinoprost (2.5 mg/0.5 ml; n = 6), or (ii) IM administration of dinoprost (25 mg/5 ml; n = 6). Concentrations of P4 were measured in plasma samples. Luteal tissue area, CLBF, and Adj. CLBF were assessed based on color Doppler ultrasonography. An intra-CL injection of dinoprost increased OT and decreased P4 levels in the jugular vein (JV) in a dose-dependent manner in cows at the mid-luteal phase. Increased CLBF and Adj. CLBF, accompanied by reduced P4 levels, were observed 2 h after intra-CL dinoprost injection in middle-stage CL. Decreased STAR and increased RIPK1 and RIPK3 mRNA levels confirmed that 2.5 mg dinoprost injected directly into CL is the minimum dose that induces luteolytic cascade. Injection of dinoprost directly into the CL (at a dosage lower than recommended for peripheral application) results in a pattern similar to IM dinoprost administration.
Autonomous Landing of a Quadrotor on a Moving Platform via Model Predictive Control
Authors: Guo Kaiyang , Tang Pan , Wang Hui , Lin Defu , Cui Xiaoxi
Published: January 2022

Landing on a moving platform is an essential requirement to achieve high-performance autonomous flight with various vehicles, including quadrotors. We propose an efficient and reliable autonomous landing system, based on model predictive control, which can accurately land in the presence of external disturbances. To detect and track the landing marker, a fast two-stage algorithm is introduced in the gimbaled camera, while a model predictive controller with variable sampling time is used to predict and calculate the entire landing trajectory based on the estimated platform information. As the quadrotor approaches the target platform, the sampling time is gradually shortened to feed a re-planning process that perfects the landing trajectory continuously and rapidly, improving the overall accuracy and computing efficiency. At the same time, a cascade incremental nonlinear dynamic inversion control method is adopted to track the planned trajectory and improve robustness against external disturbances. We carried out both simulations and outdoor flight experiments to demonstrate the effectiveness of the proposed landing system. The results show that the quadrotor can land rapidly and accurately even under external disturbance and that the terminal position, speed and attitude satisfy the requirements of a smooth landing mission.
Multi-Objective Optimization of Low Reynolds Number Airfoil Using Convolutional Neural Network and Non-Dominated Sorting Genetic Algorithm
Authors: Bakar Abu , Li Ke , Liu Haobo , Xu Ziqi , Alessandrini Marco , Wen Dongsheng
Published: January 2022

The airfoil is the prime component of flying vehicles. For low-speed flights, low Reynolds number airfoils are used. The characteristic of low Reynolds number airfoils is a laminar separation bubble and an associated drag rise. This paper presents a framework for the design of a low Reynolds number airfoil. The contributions of the proposed research are twofold. First, a convolutional neural network (CNN) is designed for the aerodynamic coefficient prediction of low Reynolds number airfoils. Data generation is discussed in detail and XFOIL is selected to obtain aerodynamic coefficients. The performance of the CNN is evaluated using different learning rate schedulers and adaptive learning rate optimizers. The trained model can predict the aerodynamic coefficients with high accuracy. Second, the trained model is used with a non-dominated sorting genetic algorithm (NSGA-II) for multi-objective optimization of the low Reynolds number airfoil at a specific angle of attack. A similar optimization is performed using NSGA-II directly calling XFOIL, to obtain the aerodynamic coefficients. The Pareto fronts of both optimizations are compared, and it is concluded that the proposed CNN can replicate the actual Pareto in considerably less time.
Identification of a New Serovar of Salmonella enterica in Mediterranean Buffalo Calves (Bubalus bubalis)
Authors: D’Angelo Luisa , Vecchio Domenico , Cozza Debora , La Tela Immacolata , Carullo Maria Rosaria , Menozzi Ilaria , Scaltriti Erika , Pongolini Stefano , Galiero Giorgio , De Carlo Esterina
Published: January 2022

This case report describes for the first-time cases of severe gastroenteritis in water buffalo calves due to a new serovar of Salmonella enterica. The study was carried out on fecal matrix collected from live water buffalo calves that showed profuse diarrhea, severe dehydration and fever, exhibiting a systemic course. Culture and molecular investigations identified the pathogens isolated from intestinal contents as two Salmonella serovars, Salmonella enterica enterica O:35 and a new serovar of Salmonella enterica. The isolates showed multi-drug resistance. Timely diagnosis associated with a targeted antimicrobial treatment were found to be sufficient for the survival and recovery of the infected animals. Herd vaccines prepared from isolated pathogens were used to prevent further deaths of the calves.
Chlorogenic Acid Improves Quality of Chilled Ram Sperm by Mitigating Oxidative Stress
Authors: Wang Yanhu , Zhang Liuming , Sohail Tariq , Kang Yan , Sun Xiaomei , Li Yongjun
Published: January 2022

The purpose of this study was to investigate whether the addition of chlorogenic acid (CGA) to a sheep semen extender could improve the quality of chilled sheep sperm. Ejaculates (n = 80) were collected from five Hu rams with an artificial vagina. The ejaculates were mixed and divided into five equal parts, diluted with a CGA-free Tris–egg yolk extender (control), or supplemented with 0.2, 0.4, 0.8, and 1.2 mg/mL. The sperm kinematic parameters (viability, progressive motility), functional integrity of plasma membrane and acrosome, adenosine triphosphate (ATP) concentration and antioxidant parameters (Catalase (CAT), Superoxide dismutase (SOD) activity, total antioxidant capacity (T-AOC), ROS level and Malondialdehyde (MDA) content) were evaluated during storage of the semen. The results indicated that: PM, plasmatic membrane integrity and acrosomal integrity in 0.8 mg/mL CGA were higher (p < 0.05) from day 1 to 5. The ROS level in CGA groups was lower than the control (p < 0.05). CAT, SOD, ATP, and T-AOC were highest at 0.8 mg/mL concentration within 1 to 5 days. The above results indicated that the right concentration of CGA improved the quality of Hu ram sperm during chilling storage.
Inbreeding Coefficient and Distance in MHC Genes of Parents as Predictors of Reproductive Success in Domestic Cat
Authors: Erofeeva Mariya N. , Alekseeva Galina S. , Kim Mariya D. , Sorokin Pavel A. , Naidenko Sergey V.
Published: January 2022

Inbreeding and low diversity in MHC genes are considered to have a negative effect on reproductive success in animals. This study presents an analysis of the number and body mass of offspring in domestic cat, depending on the inbreeding coefficient and the degree of similarity in MHC genes of class I and II in parents. Inbred partners had a lower number of live kittens at birth than outbred ones. At the same time, the inbreeding coefficient did not affect the litter size and the number of offspring who survived until the period of transition to solid food. The most significant predictor for the number of surviving offspring was the degree of parental similarity in MHC genes: the parents with the maximum distance in MHC genes had more survived kittens. Moreover, this effect was most pronounced immediately after birth. A significant percentage of kittens from parents with a minimum distance in MHC genes were either stillborn or died on the first day after birth. By the age of transition to solid food, this effect is no longer so pronounced. Furthermore, neither the inbreeding coefficient nor the distance in MHC genes of parents had any effect on the body mass of kittens.
How Do Avalanche Dogs (and Their Handlers) Cope with Physical Exercise? Heart Rate Changes during Endurance in a Snowy Environment
Authors: Menchetti Laura , Iaboni Martina , Santoro Michele Matteo , Guelfi Gabriella , Diverio Silvana
Published: January 2022

This study aimed to assess the heart rate (HR) responses of avalanche SAR dogs and handlers under working field conditions. Thirteen SAR units (dogs and handlers) performed an exercise (Endurance) consisting of approximately 5.5 km of rough tracks through deep snow, at an altitude of 1991–2250 m.a.s.l. The exercise was repeated twice for each of the two different tracks. Both handlers and dogs were equipped with a global positioning satellite/heart rate (GPS/HR) system (Polar®). Multivariable models were used to evaluate the effects of environmental (i.e., gradient, altitude, track, and time) and intrinsic (i.e., speed, repetition, and breed) factors on changes from baseline HR (Δ%HR). The dog’s Δ%HR was greater in the flat and uphill compared with downhill, and increased progressively as the speed increased (p < 0.001). Moreover, it rose at altitudes above 2100 m.a.s.l. and peaked after 30 min of the Endurance activity (p < 0.01). These findings indicated that HR monitors could be a valuable tool to contribute to the evaluation of avalanche dogs’ fitness in their real working environment. In contrast, the lack of correlation between the dogs’ and handlers’ HR changes suggests that handlers might not perceive the physical conditions of their dog in real-time. Thus, implementing protocols to monitor avalanche SAR dogs’ fitness using a GPS/HR monitoring system could help handlers to tailor the training and workload and to detect the risk factors for physical distress of working dogs.
What Drives Climate Change Adaptation Practices in Smallholder Farmers? Evidence from Potato Farmers in Indonesia
Authors: Purwanti Tina Sri , Syafrial Syafrial , Huang Wen-Chi , Saeri Mohammad
Published: January 2022

The potato is the third most consumed crop globally after rice and wheat, but climate change has often disrupted its production. Therefore, adaptation practices are needed to maintain potato productivity. This study investigates the determinants of on- and off-farm climate change adaptation practices among smallholder farmers in Indonesia, considering adaptation intensity, which has not discussed in previous literature. The cross-sectional data were collected from 302 smallholder potato farmers in East Java, Indonesia, analyzed by a multivariate probit model to estimate the determinants. An ordered probit model was subsequently employed to understand the intensity factors. The findings indicated that the significant factors that affect farmers’ choice of on-farm adaptations were the farmers’ education, their participation in farmers’ groups, agricultural-related infrastructure, and agriculture output prices. Meanwhile, the off-farm adaptations were significantly affected by the farmers’ education, employed family members, agriculture-related infrastructure, and livestock ownership. The ordered probit model also suggested that participation in farmers groups and agricultural-related infrastructure were the most significant factors that encouraged adaptation. Therefore, adaptation planning should consider these factors to optimally improve farmers’ adaptation capacity.
Early Schizophrenia and Bipolar Disorder Patients Display Reduced Neural Prepulse Inhibition
Authors: San-Martin Rodrigo , Zimiani Maria , de Ávila Milton , Shuhama Rosana , Del-Ben Cristina , Menezes Paulo , Fraga Francisco , Salum Cristiane
Published: January 2022

Background: Altered sensorimotor gating has been demonstrated by Prepulse Inhibition (PPI) tests in patients with psychosis. Recent advances in signal processing methods allow assessment of neural PPI through electroencephalogram (EEG) recording during acoustic startle response measures (classic muscular PPI). Simultaneous measurements of muscular (eye-blink) and neural gating phenomena during PPI test may help to better understand sensorial processing dysfunctions in psychosis. In this study, we aimed to assess simultaneously muscular and neural PPI in early bipolar disorder and schizophrenia patients. Method: Participants were recruited from a population-based case-control study of first episode psychosis. PPI was measured using electromyography (EMG) and EEG in pulse alone and prepulse + pulse with intervals of 30, 60, and 120 ms in early bipolar disorder (n = 18) and schizophrenia (n = 11) patients. As control group, 15 socio-economically matched healthy subjects were recruited. All subjects were evaluated with Rating Scale, Hamilton Rating Scale for Depression, and Young Mania Rating Scale questionnaires at recruitment and just before PPI test. Wilcoxon ranked sum tests were used to compare PPI test results between groups. Results: In comparison to healthy participants, neural PPI was significantly reduced in PPI 30 and PPI60 among bipolar and schizophrenia patients, while muscular PPI was reduced in PPI60 and PPI120 intervals only among patients with schizophrenia. Conclusion: The combination of muscular and neural PPI evaluations suggested distinct impairment patterns among schizophrenia and bipolar disorder patients. Simultaneous recording may contribute with novel information in sensory gating investigations.
The Role of N6-Methyladenosine (m6A) Methylation Modifications in Hematological Malignancies
Authors: Zhao Yan , Peng Hongling
Published: January 2022

Epigenetics is identified as the study of heritable modifications in gene expression and regulation that do not involve DNA sequence alterations, such as DNA methylation, histone modifications, etc. Importantly, N6-methyladenosine (m6A) methylation modification is one of the most common epigenetic modifications of eukaryotic messenger RNA (mRNA), which plays a key role in various cellular processes. It can not only mediate various RNA metabolic processes such as RNA splicing, translation, and decay under the catalytic regulation of related enzymes but can also affect the normal development of bone marrow hematopoiesis by regulating the self-renewal, proliferation, and differentiation of pluripotent stem cells in the hematopoietic microenvironment of bone marrow. In recent years, numerous studies have demonstrated that m6A methylation modifications play an important role in the development and progression of hematologic malignancies (e.g., leukemia, lymphoma, myelodysplastic syndromes [MDS], multiple myeloma [MM], etc.). Targeting the inhibition of m6A-associated factors can contribute to increased susceptibility of patients with hematologic malignancies to therapeutic agents. Therefore, this review elaborates on the biological characteristics and normal hematopoietic regulatory functions of m6A methylation modifications and their role in the pathogenesis of hematologic malignancies.