bluuebunny/crossref_metadata_2025 huggingface dataset:
Whole-Genome Metagenomic Analysis of Functional Profiles in the Fecal Microbiome of Farmed Sows with Different Reproductive Performances
Authors: Miura Hiroto , Tsukahara Takamitsu , Inoue Ryo
Published: October 2024

Recent studies suggested an association between the reproductive performance of sows and their gut microbiota. To understand how the gut microbiota affect the reproductive performances of sows, we conducted a whole-genome metagenomic analysis on the fecal microbial functional profiles of sows with high and low reproductive performances. We used 60 sows from six farms (10 sows/farm), including 30 sows from three farms with higher reproductive performances (the mean number of weaned piglets/sow/year) (group H) and 30 sows from three farms with lower performances (group L). Fecal microbial DNA was subjected to a whole-genome metagenomic analysis. Biomarker exploration analysis identified “carbohydrate transport and metabolism” as the most discriminative function enriched in group H. Further analysis of carbohydrate-active enzymes revealed that the fecal microbiome of group H had a greater capacity to degrade dietary fiber, specifically cellulose and pectin. Group H also exhibited higher fecal short-chain fatty acid (SCFA) concentrations than group L, with the abundances of cellulose- and pectin-degrading genes showing significant positive correlations with fecal SCFA concentrations. Taxonomic analysis indicated greater contributions of Prevotella, Treponema, Ruminococcus, and Fibrobacter to cellulose and pectin degradation in the fecal microbiome in group H. In conclusion, higher reproductive performances of sows were, at least in part, associated with a greater microbial capacity for degrading cellulose and pectin, resulting in a higher SCFA production in the hindgut.
Clinopyroxenite-Wehrlite Porya Guba Complex with Fe-Ti-V and PGE-Cu-Ni Mineralization in the Northeastern Part of the Fennoscandian Shield: Evidence of Post-Orogenic Formation from Sm-Nd Isotope System
Authors: Serov Pavel A. , Groshev Nikolay Yu.
Published: October 2024

The Porya Guba clinopyroxenite–wehrlite complex is located in the core of the Lapland–Kola collisional orogen (~2.0–1.9 billion years old) in the northeastern part of the Fennoscandian Shield and contains iron–titanium–vanadium and nickel–copper mineralization with platinum group elements (PGEs). The controversial geological position of the complex within the mafic granulites of the Kolvitsa mélange (pre-, syn- or post-orogenic) is clarified by Sm-Nd isotopic dating of the rocks and mineralization. The Sm-Nd age of the barren clinopyroxenites that dominate the complex is 1858 ± 34 Ma (εNd(T) = −1.5) and is interpreted as the time of its emplacement as evidenced by a sample from the largest intrusion, named Zhelezny. This age is younger than that of the peak of granulite metamorphism in the host rocks (1925–1915 Ma) and coincides within error with the age of rutile from granulites (1880–1870 Ma), indicating the time at which cooling to 450 °C occurs. Emplacement in the cooled rocks is confirmed by the detection of quenching zones in clinopyroxenites around granulite xenoliths. Magnetite ores, as well as mineralized pyroxenites with sulfide disseminations, are formed during a late stage of the complex development, as suggested by active assimilation of granulite xenoliths by these rocks. The isotopic age of mineralized pyroxenites enriched in PGEs is 1832 ± 35 Ma (εNd(T) = –2.0), while the age of magnetite ores is 1823 ± 19 Ma (εNd(T) = –2.5). Thus, the obtained isotopic data indicate that the emplacement of the Porya Guba complex and probably other small mafic–ultramafic intrusions in the Kolvitsa mélange granulites took place after the end of the Lapland–Kola collision.
Geochemical Dynamics and Evolutionary Implications of Sediments at the Xingu–Amazon Rivers’ Confluence: Proxies for Mixing, Mobility and Weathering
Authors: Medeiros Filho Lucio , Asp Nils , Lafon Jean , Souza Thiago , Berredo José , Salomão Gabriel
Published: October 2024

This study investigates the geochemical characteristics and evolutionary implications of sediments at the confluence of the Xingu and Amazon Rivers. The main objective is to understand sediment mixing, mobility, and weathering processes through geochemical proxies. Samples were collected from various sections of the lower Xingu River, focusing on its interaction with the Amazon River. Analytical techniques such as X-ray diffraction (XRD), X-ray fluorescence (XRF), and inductively coupled plasma mass spectrometry (ICP-MS) were employed to analyze major and trace elements. The results reveal significant spatial variations in mineralogical and textural patterns, with sediments forming distinct groupings based on their location. The data suggest that the lower Xingu River is strongly influenced by sediment inputs from the Amazon River, particularly affecting sediment composition and chemical weathering processes. This research highlights the critical interactions between river systems and their implications for the evolution of the Amazon basin, especially regarding sediment contributions from various geological sources. Even though the Xingu River drains cratonic regions at higher elevations, the geochemistry of the bottom sediments confirms that the bedload is derived from heterogeneous sources with primarily intermediate igneous compositions and has undergone substantial recycling during river transport.
Petrogenesis of Granitoids from the Waxing Mo Polymetallic Deposit, NE China: Implications for Magma Fertility and Mineralization
Authors: Liu Yang , Sun De-You , Gao Yang , Wang Hong-Chao , Ma Yu-Xin , Xu Jun , Liu Xin-Tong
Published: October 2024

The Waxing Mo polymetallic deposit is located in the central part of the Lesser Xing’an–Zhangguangcai Range (LXZR), NE China. The Mo (Cu) mineralization in the deposit is dominantly hosted by quartz veinlets and stockworks and is closely related to silicification and potassic alteration, while the W mineralization is most closely related to greisenization. Zircon samples from granodiorite, biotite monzogranite, granodiorite porphyry, and syenogranite in the Waxing deposit yielded U-Pb ages of 172.3 Ma, 172.8 Ma, 173.0 Ma, and 171.4 Ma, respectively. Six molybdenite samples from porphyry Mo ores yielded a Re-Os isochron age of 172.0 ± 1.1 Ma. The granitoids in the ore district are relatively high in total alkali (Na2O + K2O), are metaluminous to weakly peraluminous, and are classified as I-type granitoids. The zircon samples from all granitoids showed a relatively consistent Hf isotopic composition, as shown by positive εHf(t) values (3.1–8.3) and young TDM2 ages (0.69–1.25 Ga). These results, combined with the whole-rock geochemistry, suggest that the magma source of these rocks most likely derived from partial melting of a juvenile middle-lower continental crust, with a minor contribution from the mantle. These granitoids have compositional characteristics of adakites such as relatively high Sr contents (e.g., >400 ppm) and Sr/Y ratios (e.g., >33), as well as weak Eu anomalies (e.g., Eu/Eu* = 0.8–1.1), indicating extensive fractionation crystallization of a hydrous magma. The apatite geochemistry indicates that the ore-related magma in Waxing is F-rich and has a relatively low content of sulfur. The zircon geochemistry reveals that the granodiorite, biotite monzogranite, and granodiorite porphyry have relatively high oxygen fugacity (i.e., ΔFMQ = +1.1~1.3), whereas the fO2 values of the granite porphyry and syenogranite are relatively low (i.e., ΔFMQ = +0.1~0.5). The whole-rock and mineral geochemistry suggest that the Mo mineralization in Waxing is probably genetically related to granitoids (i.e., granodiorite, biotite monzogranite, and granodiorite porphyry), with higher oxygen fugacity and a high water content, whereas the magmatic S concentration is not the key factor controlling the mineralization. A comparison of the geochemical compositions of ore-forming and barren stocks for porphyry Mo deposits in the LXZR showed that geochemical ratios, including Eu/Eu* (>0.8), 10,000*(Eu/Eu*)/Y (>600), Sr/Y (>33), and V/Sc (>8), could be effective indicators in discriminating fertile granitoids for porphyry Mo deposits from barren ones in the region.
Application of Graphene in Acoustoelectronics
Authors: Roshchupkin Dmitry , Kononenko Oleg , Matveev Viktor , Pundikov Kirill , Emelin Evgenii
Published: October 2024

An interdigital transducer structure was fabricated from multilayer graphene on the surface of the YZ-cut of a LiNbO3 ferroelectric crystal. The multilayer graphene was prepared by CVD method and transferred onto the surface of the LiNbO3 substrate. The properties of the multilayer graphene film were studied by Raman spectroscopy. A multilayer graphene (MLG) interdigital transducer (IDT) structure for surface acoustic wave (SAW) excitation with a wavelength of Λ=60 μm was fabricated on the surface of the LiNbO3 crystal using electron beam lithography (EBL) and plasma chemical etching. The amplitude–frequency response of the SAW delay time line was measured. The process of SAW excitation by graphene IDT was visualized by scanning electron microscopy. It was demonstrated that the increase in the SAW velocity using graphene was related to the minimization of the IDT mass.
Unveiling BaTiO3-SrTiO3 as Anodes for Highly Efficient and Stable Lithium-Ion Batteries
Authors: Oli Nischal , Sapkota Nawraj , Weiner Brad R. , Morell Gerardo , S. Katiyar Ram
Published: October 2024

Amidst the swift expansion of the electric vehicle industry, the imperative for alternative battery technologies that balance economic feasibility with sustainability has reached unprecedented importance. Herein, we utilized Perovskite-based oxide compounds barium titanate (BaTiO3) and strontium titanate (SrTiO3) nanoparticles as anode materials for lithium-ion batteries from straightforward and standard carbonate-based electrolyte with 10% fluoroethylene carbonate (FEC) additive [1M LiPF6 (1:1 EC: DEC) + 10% FEC]. SrTiO3 and BaTiO3 electrodes can deliver a high specific capacity of 80 mA h g−1 at a safe and low average working potential of ≈0.6 V vs. Li/Li+ with excellent high-rate performance with specific capacity of ~90 mA h g−1 at low current density of 20 mA g−1 and specific capacity of ~80 mA h g−1 for over 500 cycles at high current density of 100 mA g−1. Our findings pave the way for the direct utilization of perovskite-type materials as anode materials in Li-ion batteries due to their promising potential for Li+ ion storage. This investigation addresses the escalating market demands in a sustainable manner and opens avenues for the investigation of diverse perovskite oxides as advanced anodes for next-generation metal-ion batteries.
Sub-ppb H2S Sensing with Screen-Printed Porous ZnO/SnO2 Nanocomposite
Authors: Akbari-Saatlu Mehdi , Heidari Masoumeh , Mattsson Claes , Zhang Renyun , Thungström Göran
Published: October 2024

Hydrogen sulfide (H2S) is a highly toxic and corrosive gas commonly found in industrial emissions and natural gas processing, posing serious risks to human health and environmental safety even at low concentrations. The early detection of H2S is therefore critical for preventing accidents and ensuring compliance with safety regulations. This study presents the development of porous ZnO/SnO2-nanocomposite gas sensors tailored for the ultrasensitive detection of H2S at sub-ppb levels. Utilizing a screen-printing method, we fabricated five different sensor compositions—ranging from pure SnO2 to pure ZnO—and characterized their structural and morphological properties through X-ray diffraction (XRD) and scanning electron microscopy (SEM). Among these, the SnO2/ZnO sensor with a composition-weight ratio of 3:4 demonstrated the highest response at 325 °C, achieving a low detection limit of 0.14 ppb. The sensor was evaluated for detecting H2S concentrations ranging from 5 ppb to 500 ppb under dry, humid air and N2 conditions. The relative concentration error was carefully calculated based on analytical sensitivity, confirming the sensor’s precision in measuring gas concentrations. Our findings underscore the significant advantages of mixture nanocomposites in enhancing gas sensitivity, offering promising applications in environmental monitoring and industrial safety. This research paves the way for the advancement of highly effective gas sensors capable of operating under diverse conditions with high accuracy.
Microwave Kinetic Inductance Detector Made of Molecular Beam Epitaxy (MBE)-Grown MgB2 Film
Authors: Roitman Ariel , Pfaff Corentin , Hauet Thomas , Shaulov Avner , Yeshurun Yosef
Published: October 2024

We present a MgB2-based Microwave Kinetic Inductance Detector (MKID) featuring a quality factor Qi ~ 105 and noise equivalent power NEP ~ 10−14 W/Hz at 2 K. In comparison to YBCO-based MKIDs, the MgB2 detector shows greater sensitivity to both temperature and magnetic field, a result of its two-gap nature and relatively low critical Hc2 field. Our data indicate that MgB2 is more advantageous for MKID applications at temperatures lower than 3 K.
Mechanical Properties of Two-Dimensional Metal Nitrides: Numerical Simulation Study
Authors: Sakharova Nataliya A. , Pereira André F. G. , Antunes Jorge M.
Published: October 2024

It is expected that two-dimensional (2D) metal nitrides (MNs) consisting of the 13th group elements of the periodic table and nitrogen, namely aluminium nitride (AlN), gallium nitride (GaN), indium nitride (InN) and thallium nitride (TlN), have enhanced physical and mechanical properties due to the honeycomb, graphene-like atomic arrangement characteristic of these compounds. The basis for the correct design and improved performance of nanodevices and complex structures based on 2D MNs from the 13th group is an understanding of the mechanical response of their components. In this context, a comparative study to determine the elastic properties of metal nitride nanosheets was carried out making use of the nanoscale continuum modelling (or molecular structural mechanics) method. The differences in the elastic properties (surface shear and Young’s moduli and Poisson’s ratio) found for the 2D 13th group MNs are attributed to the bond length of the respective hexagonal lattice of their diatomic nanostructure. The outcomes obtained contribute to a benchmark in the evaluation of the mechanical properties of AlN, GaN, InN and TlN monolayers using analytical and numerical approaches.
Anti-Calcitonin Gene-Related Peptide Monoclonal Antibody Is Effective for Preventing Migraine Aura Without Headache
Authors: Shibata Yasushi
Published: October 2024

Background: Anti-calcitonin gene-related peptide monoclonal antibodies (CGRP mAbs) are clinically effective in preventing the migraine attacks, photophobia, and migraine auras associated with headaches. However, no study has yet investigated the effectiveness of CGRP mAbs in preventing migraine aura without headache. Case report: A female patient of 49 years old presented with a long history (since age 10) of photosensitivity and typical migraine auras without a headache. The symptoms slightly responded to oral medication, lomerizine chloride, but did not completely resolve. Just one day after the administration of galcanezumab, her photo-hypersensitivity and migraine aura had completely resolved. Consequently, the administration of the oral migraine preventive medication was discontinued. Monthly galcanezumab at a dose of 120 mg was continuously given and she did not re-experience any auras or headaches. Conclusions: The use of CGRP mAbs can be considered as a potential treatment in preventing migraine aura without headache. Currently, CGRP mAb is indicated only for migraines with and without auras. Given our findings and the promising effects of this medication for this migraine subtype, a large clinical trial is required to better assess the effects and potential adverse events of CGRP mAb in patients with migraine aura without headache.
Amelioration of Cancer Cachexia by Dalbergia odorifera Extract Through AKT Signaling Pathway Regulation
Authors: Ho Phuong T. , Park Eulyong , Luong Quynh Xuan Thi , Hakim Meutia Diva , Hoang Phuong T. , Vo Thuy T. B. , Kawalin Kantawong , Kang Hee , Lee Taek-Kyun , Lee Sukchan
Published: October 2024

Background/Objectives: Cancer cachexia is a multifactorial syndrome characterized by the progressive loss of skeletal muscle mass and adipose tissue. Dalbergia odorifer is widely used in traditional medicine in Korea and China to treat various diseases. However, its exact role and underlying mechanism in regulating cancer cachexia have not been elucidated yet. This research was conducted to investigate the effect of D. odorifer extract (DOE) in preventing the development of cancer-induced cachexia symptoms and figure out the relevant mechanisms. Methods: A cancer cachexia model was established in Balb/c mice using the CT26 colon carcinoma cell line. To evaluate the anti-cachexia effect of Dalbergia odorifer extract (DOE), CT26-bearing mice were orally administered with DOE at concentrations of 50 and 100 mg/kg BW for 14 days. C2C12 myotubes and 3T3L1 adipocytes were treated with 80% CT26 conditioned medium, DOE, and wortmannin, a particular AKT inhibitor to determine the influence of DOE in the AKT signaling pathway. Mice body weight, food intake, myofiber cross-sectional area, adipocyte size, myotube diameter, lipid accumulation, and relevant gene expression were analyzed. Results: The oral administration of DOE at doses of 50 and 100 mg/kg body weight to CT26 tumor-bearing mice resulted in a significant reduction in body weight loss, an increase in food intake, and a decrease in serum glycerol levels. Furthermore, DOE treatment led to an increase in muscle mass, larger muscle fiber diameter, and elevated expression levels of MyH2 and Igf1, while simultaneously reducing the expression of Atrogin1 and MuRF1. DOE also attenuated adipose tissue wasting, as evidenced by increased epididymal fat mass, enlarged adipocyte size, and upregulated Pparγ expression, alongside a reduction in Ucp1 and IL6 levels. In cachectic C2C12 myotubes and 3T3-L1 adipocytes induced by the CT26 conditioned medium, DOE significantly inhibited muscle wasting and lipolysis by activating the AKT signaling pathway. The treatment of wortmannin, a specific AKT inhibitor, effectively neutralized DOE’s impact on the AKT pathway, myotube diameter, and lipid accumulation. Conclusions: DOE ameliorates cancer cachexia through the expression of genes involved in protein synthesis and lipogenesis, while suppressing those related to protein degradation, suggesting its potential as a plant-derived therapeutic agent in combating cancer cachexia.
Psychological Theoretical Frameworks of Healthy and Sustainable Food Choices: A Systematic Review of the Literature
Authors: Lo Dato Elena , Gostoli Sara , Tomba Elena
Published: October 2024

Background: Non-sustainable diets are associated with several environmental and health-related problems. Psychology research is interested in the study of food choice determinants, and several theoretical frameworks have been applied to study mechanisms underlying behavioral change and to develop theory-based interventions. The present systematic review is aimed at reviewing the existing literature on the psychological theoretical frameworks used to study sustainable and/or healthy food choices and their application for the development of interventions promoting such food choices, both in general and clinical populations. Methods: A systematic search of PubMed, PsycInfo, and Scopus was conducted according to PRISMA criteria. Results: Forty-five articles met the inclusion criteria and thirty-five theoretical frameworks emerged, mostly pertaining to social psychology and with the most widely used being the Theory of Planned Behavior. The majority of studies had a cross-sectional design, were conducted in general populations, and focused on healthy food choices. Only a few studies tested theory-based interventions. Internal (i.e., self-efficacy, personal values, and motivation) and external (i.e., peers, family, and social media influence) factors emerged as relevant healthy and sustainable eating determinants. Conclusions: The current review underlines that an integrative perspective combining prompts from different psychology fields is needed in order to identify the psychological factors influencing food choices and to develop psychological interventions for the promotion of more sustainable diets.
The Efficacy of Omega-3 Fatty Acids as the Monotherapy for Depression: A Randomized, Double-Blind, Placebo-Controlled Pilot Study
Authors: Wu Suet-Kei , Yang Kai-Jie , Liu Wen-Chun , Malau Ikbal Andrian , Zailani Halliru , Chang Cheng-Ho , Huang Shih-Yi , Chang Jane Pei-Chen , Chiu Wei-Che , Su Kuan-Pin
Published: October 2024

Objective: Omega-3 polyunsaturated fatty acids (n-3 PUFAs) have demonstrated protective effects in major depressive disorder (MDD) patients receiving antidepressant treatment. However, there have been a few double-blind randomized controlled trials focused on n-3 PUFAs as monotherapy in MDD, and the outcomes have been mixed. This study aimed to assess the clinical effects of n-3 PUFAs monotherapy in patients with MDD. Methods: A total of 60 patients with MDD participated in this 12-week double-blind randomized controlled trial. They were randomized to either the n-3 PUFAs group (n = 30; 3.2 g of eicosapentaenoic acid, EPA and docosahexaenoic acid, DHA per day) or the placebo group (n = 30; 3.2 g of soybean oil per day). The severity of depression was evaluated using the Hamilton Rating Scale for Depression (HRSD). Results: The n-3 PUFAs group had a significantly lower HRSD score compared with the placebo group at week 4 (p = 0.004), week 6 (p = 0.006), week 8 (p = 0.004), and week 12 (p = 0.01). The n-3 PUFAs group showed slightly higher rates for both remission (26.7% vs. 10%, p = 0.095) and response (23.3% vs. 6.7%, p = 0.145) compared with the placebo group at week 12, but these differences did not reach statistical significance. Conclusions: These findings suggested that monotherapy of n-3 PUFAs could improve depression and potentially serve as an alternative option for MDD patients.
The Incidence, Aetiology and Clinical Course of Serious Infections Complicating Biological and Targeted Synthetic Disease-Modifying Antirheumatic Drug Therapy in Patients with Rheumatoid Arthritis in Tropical Australia
Authors: Price Cody F. , Wood John P. , Ismail Ibrahim , Smith Simon , Hanson Josh
Published: October 2024

Introduction: Patients receiving biological and targeted synthetic disease-modifying antirheumatic drugs (b/tsDMARDs) for rheumatological conditions are at an increased risk of serious, potentially life-threatening, infection. However, the incidence, aetiology, and clinical course of serious infection in patients receiving b/tsDMARDs in tropical settings are incompletely defined. Methods: We retrospectively reviewed all patients with rheumatoid arthritis receiving b/tsDMARDs between October 2012 and October 2021, at Cairns Hospital in tropical Australia. The incidence, aetiology, and clinical course of serious infections (those requiring admission to hospital or parenteral antibiotics) were determined. Results: 310 patients had 1468 patient years of b/tsDMARD therapy during the study period; 74/310 (24%) had 147 serious infections translating to an overall risk of 10.0 episodes of serious infection per 100 patient years. The respiratory tract (50/147, 34%) and skin (37/147, 25%) were the most frequently affected sites. A pathogen was identified in 59/147 (40%) episodes and was most commonly Staphylococcus aureus (24/147, 16%). Only 2/147 (1%) were confirmed “tropical infections”: 1 case of Burkholderia pseudomallei and 1 case of mixed B. pseudomallei and community-acquired Acinetobacter baumannii infection. Overall, 13/147 (9%) episodes of serious infection required Intensive Care Unit admission (0.9 per 100-patient years of b/tsDMARD therapy) and 4/147 (3%) died from their infection (0.3 per 100-patient years of b/tsDMARD therapy). The burden of comorbidity and co-administration of prednisone were the strongest predictors of death or a requirement for ICU admission. Conclusions: The risk of serious infection in patients taking b/tsDMARDs in tropical Australia is higher than in temperate settings, but this is not explained by an increased incidence of traditional tropical pathogens.
Physically Cross-Linked PVA Hydrogels as Potential Wound Dressings: How Freezing Conditions and Formulation Composition Define Cryogel Structure and Performance
Authors: Górska Anna , Baran Ewelina , Knapik-Kowalczuk Justyna , Szafraniec-Szczęsny Joanna , Paluch Marian , Kulinowski Piotr , Mendyk Aleksander
Published: October 2024

Objectives: Hydrogels produced using the freeze–thaw method have demonstrated significant potential for wound management applications. However, their production requires precise control over critical factors including freezing temperature and the choice of matrix-forming excipients, for which no consensus on the optimal conditions currently exists. This study aimed to address this gap by evaluating the effects of the above-mentioned variables on cryogel performance. Methods: Mechanical properties, absorption capacity, and microstructure were assessed alongside advanced analyses using differential scanning calorimetry (DSC) and low-field nuclear magnetic resonance relaxometry (LF TD NMR). Results: The results demonstrated that fully hydrolyzed polyvinyl alcohol (PVA) with a molecular weight above 61,000 g/mol is essential for producing high-performance cryogels. Among the tested formulations, an 8% (w/w) PVA56–98 solution (Mw~195,000; DH = 98.0–98.8%) with 10% (w/w) propylene glycol (PG) provided the best balance of stretchability, durability, and low adhesion. Notably, while −25 °C is often used for cryogel preparation, freezing the gel precursor at −80 °C yielded superior results, producing materials with more open, interconnected structures and enhanced mechanical strength and elasticity—deviating from conventional practices. Conclusions: The designed cryogel prototypes exhibited functional properties comparable to or even surpassing commercial wound dressings, except for absorption capacity, which remained lower. Despite this, the cryogel prototypes demonstrated potential as wound dressings, particularly for use in dry or minimally exuding wounds. All in all, this study provides a comprehensive analysis of the physicochemical and functional properties of PVA cryogels, establishing a strong foundation for the development of advanced wound dressing systems.
Enhanced Ocular Drug Delivery of Dexamethasone Using a Chitosan-Coated Soluplus®-Based Mixed Micellar System
Authors: Adwan Samer , Al-Akayleh Faisal , Qasmieh Madeiha , Obeidi Teiba
Published: October 2024

Background: This study introduces a novel dexamethasone (DEX) mixed micellar system (DEX-MM) using Soluplus® and Pluronic F-127 (PF127) to enhance ocular drug delivery. The enhancement of ocular application properties was achieved by creating a chitosan-coated DEX-MM (DEX-CMM), which promotes better adherence to the ocular surface, thereby improving drug absorption. Methods: Using the solvent evaporation method, a formulation was developed with a Soluplus®-to-drug ratio of 1:10, enhanced with 0.25% PF127. After dispersing in water, 1% chitosan (CS) was added. The stability and integrity of DEX within the micelles were verified using attenuated total reflection–Fourier transform infrared spectroscopy (ATR-FTIR) and differential scanning calorimetry (DSC). Additionally, in vitro and ex vivo drug release studies were conducted. Results: DEX-CMM (F6) demonstrated a particle size of 151.9 ± 1 nm and a polydispersity index (PDI) of 0.168 ± 0.003, suggesting uniformity and high electrostatic stability with a zeta potential of +35.96 ± 2.13 mV. The non-Fickian drug release mechanism indicated prolonged drug retention. Comparative analyses showed DEX-CMM outperforming a standard DEX suspension in drug release and ocular tissue permeation, with flux measurements significantly higher than the DEX suspension. Conclusion: The study confirmed the efficacy of DEX-CMM in enhancing drug delivery to ocular tissues, evidenced by improved permeability. Safety evaluations using the HET-CAM test demonstrated that DEX-CMM was non-irritant, supporting its potential for effective ocular drug delivery.
Pharmacological Treatment of Interstitial Lung Diseases: A Novel Landscape for Inhaled Agents
Authors: D’Agnano Vito , Perrotta Fabio , Fomez Ramona , Carrozzo Valerio Maria , Schiattarella Angela , Sanduzzi Zamparelli Stefano , Pagliaro Raffaella , Bianco Andrea , Mariniello Domenica Francesca
Published: October 2024

Interstitial lung diseases (ILDs) encompass a heterogeneous group of over 200 disorders that require individualized treatment. Antifibrotic agents, such as nintedanib and pirfenidone, have remarkably revolutionized the treatment landscape of patients with idiopathic pulmonary fibrosis (IPF). Moreover, the approval of nintedanib has also expanded the therapeutic options for patients with progressive pulmonary fibrosis other than IPF. However, despite recent advances, current therapeutic strategies based on antifibrotic agents and/or immunomodulation are associated with non-negligible side effects. Therefore, several studies have explored the inhalation route aiming to spread higher local concentrations while limiting systemic toxicity. In this review, we examined the currently available literature about preclinical and clinical studies testing the efficacy and safety of inhalation-based antifibrotics, immunomodulatory agents, antioxidants, mucolytics, bronchodilators, and vasodilator agents in ILDs.
Minimal Information for Studies of Extracellular Vesicles (MISEV): Ten-Year Evolution (2014–2023)
Authors: Zhang Yuan , Lan Mengyi , Chen Yong
Published: October 2024

In the tenth year since the first edition of MISEV was released in 2014, MISEV2023 has been reported in 2024 with the aim of refining the standard and improving the rigor, reproducibility, and transparency of extracellular vesicle (EV) research to clarify the requirements for experimental design of EVs, emphasize the importance of reproducible experimental results as well as encouraging openness of experimental information. The release of MISEV has significantly contributed to the quality of research in the field of EVs, which creates a more reliable research environment. However, despite the important role of MISEV, there is still a need for the EV researchers to continue to push for the widespread implementation of the guidelines to meet the evolving nature and challenges of EV research. The evolution of EV research and the attention it receives have grown exponentially over time, as has the number of people involved in the writing of MISEV. Here, this review briefly summarizes the evolution of the three editions of MISEV, aiming to recall MISEV2014 and MISEV2018 while learning about the latest release, MISEV2023, to gain a deeper understanding of the content, and to provide a quick note for beginners who want to learn about MISEV and explore the EV world.
Targeted Magnetic Nanoparticles for Beta-Amyloid Detection
Authors: Chmelyuk Nelly S. , Nikitin Aleksey A. , Vadekhina Veronika V. , Mitkevich Vladimir A. , Abakumov Maxim A.
Published: October 2024

Background/Objectivities: The presence of beta-amyloid plaques is a part of the pathogenesis of Alzheimer’s disease, but there is currently no universally accepted method for magnetic resonance (MR) imaging of the disease. However, it is known that magnetic nanoparticles (MNPs) can improve the T2 contrast in MR images of various targets. Methods: We used cubic MNPs, which were produced by thermal decomposition and then it was covalently bonded to a modified fluorescently labeled tetrapeptide, HAEE-Cy5, for visualizing beta-amyloid plaques. The interaction of MNPs-HAEE-Cy5 and beta-amyloid was determinate by confocal microscopy using SH-SY5Y cell line. Results: MNPs exhibit relatively high relaxivity (approximately 200 mM−1s−1), which is crucial for enhancing target visibility in MR imaging. HAEE provides targeted delivery of MNPs by specifically interacting with beta-amyloid, while the fluorescent label Cy5 enables monitoring the efficacy of the interaction through confocal microscopy. Conclusions: The MNPs modified with HAEE-Cy5 demonstrated excellent binding to beta-amyloid plaques in vitro, as shown by experiments on the SH-SY5Y cell line. These results suggest that the proposed method has potential for use in future MR imaging studies of Alzheimer’s disease.
Pharmacy Education and Clinical Pharmacy Training in France
Authors: Ranchon Florence , Chanoine Sébastien ,   ANEPC Pedagogical Committee, Dupuis Antoine , Grimandi Gaël , Sève Michel , Honoré Stéphane , Allenet Benoît , Bedouch Pierrick
Published: October 2024

Clinical pharmacy education varies widely between European countries, and several major changes have taken place in France. This review aims to describe the current state of pharmacy education in France, focusing on clinical pharmacy. Research into legislative texts on pharmacy education in France was conducted based on the national database “legifrance”. A complementary search on clinical pharmacy teaching methods used in France was carried out on the Medline, Embase, Pascal and Francis database for articles published from 2008 to 30 April 2021. Pharmacy studies are taught in universities and last from six to ten years, depending on the student’s chosen options. The scientific curriculum is defined at the national level. Students choose their professional path after the fourth year with specialized courses. Whatever the direction chosen, all students have several internships, including a half-time one-year hospital internship, with patient-centered hospital functions within medical and pharmaceutical teams. The status of clinical pharmacy has been enhanced under French law and regulations, improving clinical pharmacy education, which is now skill-based, in a progressive, active, and dynamic process, with community or hospital pharmacists as university teachers and closer to real-life clinical pharmacy. Teaching is increasingly innovative, and this needs to be shared and reported in the literature. Several important reforms have modernized French pharmacy studies in recent years, conferring a pivotal place for clinical pharmacy.
Online Optical Axis Parallelism Measurement Method for Continuous Zoom Camera Based on High-Precision Spot Center Positioning Algorithm
Authors: Kang Chanchan , Fang Yao , Wang Huawei , Zhou Feng , Ren Zeyue , Han Feixiang
Published: October 2024

Ensuring precise alignment of the optical axis is critical for achieving high-quality imaging in continuous zoom cameras. However, existing methods for measuring optical axis parallelism often lack accuracy and fail to assess parallelism across the entire focal range. This study introduces an online measurement method designed to address these limitations by incorporating two enhancements. First, image processing methodologies enable sub-pixel-level extraction of the spot center, achieved through improved morphological processing and the incorporation of an edge tracing algorithm. Second, measurement software developed using Qt Creator can output real-time data on optical axis parallelism across the full focal range post-measurement. This software features a multi-threaded architecture that facilitates the concurrent execution of image acquisition, data processing, and serial communication. Experimental results derived from simulations and real data indicate that the maximum average error in extracting the center of the spot is 0.13 pixels. The proposed system provides critical data for optical axis calibration during camera adjustment and inspection.
Generation of Wideband Signals Based on Continuous-Time Photonic Compression
Authors: Zhou Zhen , Zhang Yukang , Chi Hao
Published: October 2024

A detailed study on continuous-time photonic compression (CTPC) for generating wideband signals is presented in this paper. CTPC enables the conversion of parallel analog waveforms from multiple channels into a time-compressed continuous-time waveform with increased bandwidth. We demonstrate a CTPC system with a compression factor of two in a proof-of-concept experiment. Subsequently, the origin of the distortion in the generated signals is investigated, and we proposed a method based on bandpass filtering to remove the periodic dips observed in the generated waveforms. In addition, a predistortion method is proposed to eliminate the distortion caused by the non-ideal spectral property of the multichannel system. Further simulation results are presented to show the potential of the proposed approach.
The Effect of Post-Exercise Warm Water Immersion on Anaerobic Power Performance in College Students
Authors: Newlands Chloe E. , Aquino Michele , Young Kevin Z. , Gupta Soniya T. , Petrizzo John , Wygand John W. , Otto Robert M.
Published: October 2024

Background/Objectives: The purpose of this study was to investigate the effect of a short-term passive post-exercise heat acclimation (HA) protocol on anaerobic performance in college students. Methods: In total, 28 participants (age 22.3 ± 2.1 years, height 168 ± 9.1 cm, mass 71.5 ± 16.6 kg) volunteered and were randomly divided into either an intervention (INT) or control (CON) group. Both groups followed a consecutive two-day protocol, consisting of a 25 min submaximal cycle @ 50–60% heart rate reserve (HRR), followed by a 25 min hot water immersion (INT) or nothing (CON). All participants performed a 30 s Wingate Anaerobic Test (WAnT) on four separate occasions—familiarization, pre-intervention, acutely post-intervention, and 24 h post intervention. The WAnT measured their peak power (W), relative peak power (W/kg), mean power (W), fatigue index (%), and total work (kJ). Results: From the results of the Factorial ANOVA, it was found that there were no significant differences, with no main effect for group (INT vs. CON) or time (Trials 1–4) and no significant interaction across all measured variables (p > 0.05). Conclusions: A two-day post-exercise passive HA protocol utilizing hot water immersion had no significant effect on the measurement of anaerobic performance in college students.
Ecophysiological Trade-Off Strategies of Three Gramineous Crops in Response to Root Extracts of Phytolacca americana
Authors: Wang Xinyu , Cao Yuting , Jin Yefei , Sun Lifu , Tang Fangping , Dong Lijia
Published: October 2024

The invasive Phytolacca americana L. poses a significant threat to local agroforestry ecosystems due to its allelopathic toxicity. However, the ecophysiological response mechanisms of crops to allelochemicals remain unclear. This study investigated the seedling growth, physiological, and biochemical responses of three gramineous crops to the root extracts of P. americana and identified potential allelochemicals of the invader. The germination and seedling growth of three crops were inhibited by extracts differently, with high-concentration extracts causing more severe inhibition on seedling roots in hydroponic (>57%) than soil culture experiments (>18%). This inhibition may be related to representative secondary metabolites such as fatty acyls, alkaloids, and phenols. Despite the significant inhibition of high-concentration extracts on seedling growth, the levels of soluble sugar, soluble protein, and antioxidant enzymes increased synergistically. Under allelopathic stress, three species enhanced antioxidant enzyme activities and metabolite contents at the cost of reducing their shoot, root biomass, and root/shoot ratio. This may be an ecophysiological growth-defense strategy to bolster their resistance to allelopathy. Interestingly, transgenic rice exhibited greater sensitivity to allelochemicals than wild-type rice, resulting in more pronounced growth inhibition and increased levels of most metabolites and antioxidant enzymes. This study highlights the adaptive strategies of three gramineous crops to the allelopathy of invasive P. americana.
Brassinosteroids Alleviate Salt Stress by Enhancing Sugar and Glycine Betaine in Pepper (Capsicum annuum L.)
Authors: Jin Yujie , Yang Ping , Li Jian , Yang Yongchao , Yang Ruopeng , Fu Hongbo , Li Jie
Published: October 2024

Salt stress is a major abiotic factor that negatively impacts the growth, performance, and secondary metabolite production in pepper (Capsicum annuum L.) plants. Brassinosteroids (BRs) play a crucial role in enhancing plant tolerance to abiotic stress, yet their potential in mitigating salt stress in pepper plants, particularly by promoting sugar and glycine betaine accumulation, remains underexplored. In this study, we investigated the effects of the foliar application of 2,4-epibrassinolide (EBR) on salt-stressed pepper seedlings. Our findings revealed that EBR treatment significantly increased the levels of proline, sugar, and glycine betaine under salt stress compared to untreated controls. Moreover, EBR enhanced the antioxidant defense mechanisms in pepper seedlings by increasing sugar and glycine betaine levels, which contributed to the reduction of reactive oxygen species (ROS) and malondialdehyde (MDA) accumulation.
Induced Electron Traps via the PCBM in P(VDF-HFP) Composites to Enhance Dielectric and Energy Storage Performance
Authors: Yang Yantao , Qiao Jingqi , Sun Haiyu , Yang Wenhao , Wei Liangliang , Zhao Xuetong
Published: October 2024

Polymer-based composites with excellent dielectric properties are essential for advanced energy storage applications. In this work, the [6,6]-phenyl-C61-butyric acid methyl ester (PCBM) as a filler was incorporated into the poly(vinylidene fluoride-co-hexafluoropropylene) (P(VDF-HFP)) composite to improve its dielectric performance. P(VDF-HFP) composite films with varying PCBM concentrations were prepared via solution casting and their dielectric, energy storage, and charge–discharge properties were characterized. It was found that the doped PCBM could introduce new charge traps with an energy level of 1.25 eV that modulate charge transport and energy storage characteristics of the polymer matrix. The dielectric constant of the composites was enhanced to the maximum of 10.87 as 0.2 vol% PCBM was added, while the breakdown strength reached 455 MV/m, achieving an energy density of 7.38 J/cm3, which is 33% higher than the pristine P(VDF-HFP) film. Furthermore, the charge–discharge efficiency of the composites was enhanced 66% under the electric field of 300 MV/m. These results demonstrate that PCBM significantly improves the dielectric and energy storage properties of P(VDF-HFP) composites, providing a promising approach for the development of high-performance dielectric materials in flexible energy storage devices.
Mechanical Strength of Additive Manufactured and Standard Polymeric Components Joined Through Structural Adhesives
Authors: Spaggiari Andrea , Orlandini Simone
Published: October 2024

The main aim of this work is to evaluate the mechanical properties of additive manufactured polymeric parts joined with standard plastic parts through structural adhesives. The primary advantage of this technique is its ability to significantly increase the size of the final assembly by using additive manufacturing (AM) for complex joints and inexpensive, reliable extruded plastic parts for load-bearing components. This hybrid assembly combines the flexibility and shape adaptability of AM with the structural strength and cost-effectiveness of extruded polymer parts, resulting in a final design that performs comparably to the base material. The materials used in the paper are rigid acrylic adhesive and toughened acrylic, both applicable with almost no surface preparation and fast curing. The 3D-printed parts are produced in ABS, while the standard parts are in PVC. First, the work is devoted to estimating the performance of the adhesives using pin–collar joints and a combined numerical and experimental methodology. The second section presents and discusses the results of two more realistic applications of adhesive bonding to hybrid complex joints. For the pin–collar joints, the results show failure mostly in the adhesive, with an average shear stress of 11.5 MPa and 5.22 MPa and a stiffness of 4449 N/mm and 3649 N/mm for the rigid and toughened adhesives, respectively. The results of the adhesive bonding of structural joints show that the adhesive is always capable of providing the load-carrying capacity required to achieve the strength of traditionally manufactured polymeric parts. The paper shows that adhesives are a feasible way to expand the potential of 3D-printed equipment to obtain larger hybrid parts partially realized with traditional technology, especially with inexpensive off-the-shelf bars and sections.
Highly Porous 3D Nanofibrous Scaffold of Polylactic Acid/Polyethylene Glycol/Calcium Phosphate for Bone Regeneration by a Two-Step Solution Blow Spinning (SBS) Facile Route
Authors: da Silva Vanderlane Cavalcanti , Gomes Déborah dos Santos , de Medeiros Eudes Leonan Gomes , Santos Adillys Marcelo da Cunha , de Lima Isabela Lemos , Rosa Taciane Pedrosa , Rocha Flaviana Soares , Filice Leticia de Souza Castro , Neves Gelmires de Araújo , Menezes Romualdo Rodrigues
Published: October 2024

This work presents the successful production of highly porous 3D nanofibrous hybrid scaffolds of polylactic acid (PLA)/polyethylene glycol (PEG) blends with the incorporation of calcium phosphate (CaP) bioceramics by a facile two-step process using the solution blow spinning (SBS) technique. CaP nanofibers were obtained at two calcium/phosphorus (Ca/P) ratios, 1.67 and 1.1, by SBS and calcination at 1000 °C. They were incorporated in PLA/PEG blends by SBS at 10 and 20 wt% to form 3D hybrid cotton-wool-like scaffolds. Morphological analysis showed that the fibrous scaffolds obtained had a randomly interconnected and highly porous structure. Also, the mean fiber diameter ranged from 408 ± 141 nm to 893 ± 496 nm. Apatite deposited considerably within 14 days in a simulated body fluid (SBF) test for hybrid scaffolds containing a mix of hydroxyapatite (HAp) and tri-calcium phosphate-β (β-TCP) phases. The scaffolds with 20 wt% CaP and a Ca/P ration of 1.1 showed better in vitro bioactivity to induce calcium mineralization for bone regeneration. Cellular tests evidenced that the developed scaffolds can support the osteogenic differentiation and proliferation of pre-osteoblastic MC3T3-E1 cells into mature osteoblasts. The results showed that the developed 3D scaffolds have potential applications for bone tissue engineering.
Numerical Modeling of Hydraulic Fracturing Interference in Multi-Layer Shale Oil Wells
Authors: Guo Xinwei , Aibaibu Abulimiti , Wu Yuezhong , Chen Bo , Zhou Hua , Zhu Bolong , Zhao Xiangyun
Published: October 2024

Multi-layer horizontal well development and hydraulic fracturing are key techniques for enhancing production from shale oil reservoirs. During well development, the fracturing performance and well-pad production are affected by depletion-induced stress changes. Previous studies generally focused on the stress and fracturing interference within the horizontal layers, and the infilled multi-layer development was not thoroughly investigated. This study introduces a modeling workflow based on finite element and displacement discontinuity methods that accounts for dynamic porous media flow, geomechanics, and hydraulic fracturing modeling. It quantitatively characterizes the in situ stress alteration in various layers caused by the historical production of parent wells and quantifies the hydraulic fracturing interference in infill wells. In situ stress changes and reorientation and the non-planar propagation of hydraulic fractures were simulated. Thus, the workflow characterizes infill-well fracturing interferences in shale oil reservoirs developed by multi-layer horizontal wells. Non-planar fracturing in infill wells is affected by the parent-well history production, infilling layers, and cluster number. They also affect principal stress reorientations and reversal of the fracturing paths. Interwell interference can be decreased by optimizing the infilling layer, infill-well fracturing timing, and cluster numbers. This study extends the numerical investigation of interwell fracturing interference to multi-layer development.