bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-Indices Diagnosis of the Conditions That Led to the Two 2017 Major Wildfires in Portugal
Authors: Andrade Cristina , Bugalho Lourdes
Published: February 2023

Forest fires, though part of a natural forest renewal process, when frequent and on a large -scale, have detrimental impacts on biodiversity, agroforestry systems, soil erosion, air, and water quality, infrastructures, and the economy. Portugal endures extreme forest fires, with a record extent of burned areas in 2017. These complexes of extreme wildfire events (CEWEs) concentrated in a few days but with highly burned areas are, among other factors, linked to severe fire weather conditions. In this study, a comparison between several fire danger indices (named ‘multi-indices diagnosis’) is performed for the control period 2001–2021, 2007 and 2017 (May–October) for the Fire Weather Index (FWI), Burning Index (BI), Forest Fire Danger Index (FFDI), Continuous Haines Index (CHI), and the Keetch–Byram Drought Index (KBDI). Daily analysis for the so-called Pedrógão Grande wildfire (17 June) and the October major fires (15 October) included the Spread Component (SC), Ignition Component (IC), Initial Spread Index (ISI), Buildup Index (BUI), and the Energy Release Component (ERC). Results revealed statistically significant high above-average values for most of the indices for 2017 in comparison with 2001–2021, particularly for October. The spatial distribution of BI, IC, ERC, and SC had the best performance in capturing the locations of the two CEWEs that were driven by atmospheric instability along with a dry environment aloft. These results were confirmed by the hotspot analysis that showed statistically significant intense spatial clustering between these indices and the burned areas. The spatial patterns for SC and ISI showed high values associated with high velocities in the spread of these fires. The outcomes allowed us to conclude that since fire danger depends on several factors, a multi-indices diagnosis can be highly relevant. The implementation of a Multi-index Prediction Methodology should be able to further enhance the ability to track and forecast unique CEWEs since the shortcomings of some indices are compensated by the information retrieved by others, as shown in this study. Overall, a new forecast method can help ensure the development of appropriate spatial preparedness plans, proactive responses by civil protection regarding firefighter management, and suppression efforts to minimize the detrimental impacts of wildfires in Portugal.
Transcriptomic and Metabolomic Analyses of Palaemon carinicauda Hepatopancreas in Response to Enterocytozoon hepatopenaei (EHP) Infection
Authors: Hu Guangwei , Wang Weili , Xu Kai , Wang Chao , Liu Dexue , Xu Jing , Yan Binlun , Ji Nanjing , Gao Huan
Published: February 2023

Enterocytozoon hepatopenaei (EHP), a microsporidian responsible for hepatopancreatic microsporidiosis, is a major pathogen in commercial shrimp production. Among the affected species, Palaemon carinicauda (formerly Exopalaemon carinicauda) is commercially important in China and represents a potential research model for studying crustaceans. However, little information is available on its response to EHP infection. Hence, this study analyzed the transcriptome and metabolome of P. carinicauda’s hepatopancreas using high-throughput sequencing and liquid chromatograph-mass spectrometry (LC-MS) to determine its response during the early stage of infection. The transcriptomic analyses identified 730 differentially expressed genes, of which those associated with EHP infection were enriched in metabolic pathways as well as detoxification and antioxidant pathways. In addition, 144 differential metabolites were identified using a combination of positive and negative ion modes in LC-MS. The Kyoto encyclopedia of genes and genomes pathway analyses further indicated that the degradation of aromatic compounds, the AMP-activated protein kinase signaling pathway and C5-branched dibasic acid metabolism were significantly enriched after EHP infection. These results could provide useful insights into the effects of EHP on shrimps during the early stages of infection and help to understand the mechanisms underlying the stunted growth of shrimps after infection.
Numerical Study of Flow Downstream a Step with a Cylinder Part 1: Validation of the Numerical Simulations
Authors: Abdollahpour Milad , Gualtieri Paola , Vetsch David F. , Gualtieri Carlo
Published: February 2023

The backward-facing step flow (BFSF) is a classical problem in fluid mechanics, hydraulic engineering, and environmental hydraulics. The nature of this flow, consisting of separation and reattachment, makes it a problem worthy of investigation. In this study, divided into two parts, the effect of a cylinder placed downstream of the step on the 2D flow structure was investigated. In Part 1, the classical 2D BFSF was validated by using OpenFOAM. The BFSF characteristics (reattachment, recirculation zone, velocity profile, skin friction coefficient, and pressure coefficient) were validated for a step-height Reynolds number in the range from 75 to 9000, covering both laminar and turbulent flow. The numerical results at different Reynolds numbers of laminar flow and four RANS turbulence models (standard k-ε, RNG k-ε, standard k-ω, and SST k-ω) were found to be in good agreement with the literature data. In laminar flow, the average error between the numerical results and experimental data for velocity profiles and reattachment lengths and the skin friction coefficient were lower than 8.1, 18, and 20%, respectively. In turbulent flow, the standard k-ε was the most accurate model in predicting pressure coefficients, skin friction coefficient, and reattachment length with an average error lower than 20.5, 17.5, and 6%, respectively. In Part 2, the effect on the 2D flow structure of a cylinder placed at different horizontal and vertical locations downstream of the step was investigated.
New Fractional Cancer Mathematical Model via IL-10 Cytokine and Anti-PD-L1 Inhibitor
Authors: Uçar Esmehan , Özdemir Necati
Published: February 2023

In this study, we explore a recent biological model created to analyze the behavior of cancer cells by administering a dose of a drug containing anti-PD-L1 and IL-10 with the Caputo and Atangana–Baleanu derivative in the Caputo sense (ABC). Using the Caputo derivative in order to examine the stability of the non-linear system, we are able to demonstrate that it is existent and unique, and to introduce several numeric data obtained for the fractional values in MATLAB by using the Adams–Bashforth–Moulton (ABM) method. Additionally, by using the predictor–corrector approach, the numerical results from the system with ABC derivative will be produced. As a result, it has been observed that immune system cells that are exposed to single-dose drug with fractional order effectively combat cancer cells. The tumor cells decrease by 70.44% and 80.16% for the system generalized by the Caputo and ABC derivative, respectively, for the order α=0.42.
Characteristics of Sasakian Manifolds Admitting Almost ∗-Ricci Solitons
Authors: Rovenski Vladimir , Patra Dhriti Sundar
Published: February 2023

This article presents some results of a geometric classification of Sasakian manifolds (SM) that admit an almost ∗-Ricci soliton (RS) structure (g,ω,X). First, we show that a complete SM equipped with an almost ∗-RS with ω≠ const is a unit sphere. Then we prove that if an SM has an almost ∗-RS structure, whose potential vector is a Jacobi vector field on the integral curves of the characteristic vector field, then the manifold is a null or positive SM. Finally, we characterize those SM represented as almost ∗-RS, which are ∗-RS, ∗-Einstein or ∗-Ricci flat.
Bifurcation and Analytical Solutions of the Space-Fractional Stochastic Schrödinger Equation with White Noise
Authors: Al Nuwairan Muneerah
Published: February 2023

The qualitative theory for planar dynamical systems is used to study the bifurcation of the wave solutions for the space-fractional nonlinear Schrödinger equation with multiplicative white noise. Employing the first integral, we introduce some new wave solutions, assorted into periodic, solitary, and kink wave solutions. The dependence of the solutions on the initial conditions is investigated. Some solutions are clarified by the display of their 2D and 3D representations with varying levels of noise to show the influence of multiplicative white noise on the solutions.
Minkowski–Sierpinski Fractal Structure-Inspired 2 × 2 Antenna Array for Use in Next-Generation Wireless Systems
Authors: Vallappil Arshad Karimbu , Khawaja Bilal A. , Rahim Mohamad Kamal A. , Uzair Muhammad , Jamil Mohsin , Awais Qasim
Published: February 2023

In this paper, the design, simulation, fabrication, and characterization study of a low-cost and directional hybrid four-element (2 × 2 configuration) Minkowski–Sierpinski fractal antenna array (MSFAA) for the high-efficiency IEEE 802.11ax WLANs (Wi-Fi 6E) and the sub-6 GHz 5G wireless system is presented. Each element of the array is separated by 0.7 λ0. The complete four-element fractal antenna array system includes designing the single-element Minkowski–Sierpinski fractal antenna using two different substrates for performance comparison and an equal-split Wilkinson power divider (WPD) to achieve power division and to form a feed network. The single-element antenna, four-element fractal antenna array, and WPDs are fabricated using a flame-resistant (FR4) glass epoxy substrate with a dielectric constant (εr) of 4.3 and thickness (h) of 1.66 mm. For performance comparison, a high-end Rogers thermoset microwave material (TMM4) substrate is also used, having εr = 4.5 and h = 1.524mm, respectively. The designed four-element fractal antenna array operates at the dual-band frequencies of 4.17 and 5.97 GHz, respectively. The various performance parameters of the antenna array, such as return loss, bandwidth, gain, and 2D and 3D radiation patterns, are analyzed using CST Microwave Studio. The fabricated four-element antenna array provides the bandwidth and gain characteristic of 85 MHz/4.19 dB and 182 MHz/9.61 dB at 4.17 and 5.97 GHz frequency bands, respectively. The proposed antenna array design gives an improvement in the bandwidth, gain, and radiation pattern in the boresight at both frequencies. In the IEEE 802.11 ax WLANs (Wi-Fi 6E) deployments and the upcoming 5G wireless and satellite communication systems, it is critical to have directional antenna arrays to focus the radiated power in any specific direction. Therefore, it is believed that the proposed dual-band four-element fractal antenna array with directional radiation patterns can be an ideal candidate for the high-efficiency IEEE 802.11ax WLANs (Wi-Fi 6E) and the upcoming 5G wireless and satellite communication systems.
Resveratrol-Loaded Chia Seed Oil-Based Nanogel as an Anti-Inflammatory in Adjuvant-Induced Arthritis
Authors: Afzal Obaid , Altamimi Abdulamalik S. A. , Alamri Mubarak A. , Altharawi Ali , Alossaimi Manal A. , Akhtar Md Sayeed , Tabassum Fauzia , Almalki Waleed H. , Singh Tanuja
Published: February 2023

Natural anti-inflammatory nutraceuticals may be useful in preventing rheumatoid arthritis from worsening. Resveratrol (RV) and chia seed oil, having antioxidant potential, can assist in avoiding oxidative stress-related disorders. This investigation developed and evaluated resveratrol-loaded chia seed oil-based nanoemulsion (NE) gel formulations through in vitro and in vivo studies. The physical stability and in vitro drug permeability of the chosen formulations (NE1 to NE10) were studied. The optimized RV-loaded nanoemulsion (NE2) had droplets with an average size of 37.48 nm that were homogeneous in shape and had a zeta potential of −18 mV. RV-NE2, with a permeability of 98.21 ± 4.32 µg/cm2/h, was gelled with 1% carbopol-940P. A 28-day anti-arthritic assessment (body weight, paw edema, and levels of pro-inflammatory mediators including TNF-α, IL-6, IL-1β, and COX-2) following topical administration of RV-NE2 gel showed significant reversal of arthritic symptoms in arthritic Wistar rats induced by Freund’s complete adjuvant injection. Therefore, RV-NE2 gel demonstrated the potential to achieve local therapeutic benefits in inflammatory arthritic conditions due to its increased topical bioavailability and balancing of pro-inflammatory mediators.
Development of Adapalene Loaded Liposome Based Gel for Acne
Authors: Arooj Asma , Rehman Asim Ur , Iqbal Muhammad , Naz Iffat , Alhodaib Aiyeshah , Ahmed Naveed
Published: February 2023

Retinoids are considered the mainstay treatment for moderate to severe acne. Adapalene, a third-generation retinoid, has physiochemical properties which hinder the effective delivery of the drug to the skin. Therefore, the current study aimed to develop and evaluate adapalene liposomal loaded gel (ADA-LP gel) for the effective management of acne to improve tolerability and delivery to targeted sites as compared to the conventional dosage form of the drug. A novel spontaneous phase transition method (SPT) was used to formulate liposomes. Liposomal formulation (ADA-LP) was prepared and optimized based on particle size, zeta potential, and PDI. Optimized formulation was further characterized by different techniques and loaded into Carbopol gel. In vitro drug release, ex vivo permeation, and in vivo studies were performed using the prepared adapalene-loaded liposomal-based gel. The in vivo study was done employing the testosterone-induced acne model in mice. The optimized formulation had a size of 181 nm, PDI 0.145, and a zeta potential of −35 mV, indicating that the formulation was stable. Encapsulation efficiency was 89.69 ± 0.5%. ADA-LPs were loaded into the gel. Prepared ADA-LP showed a 79 ± 0.02% release of drug in a sustained manner, within 24 h. The ex vivo permeability study showed a total of 43 ± 0.06 µg/cm2 of drug able to permeate through the skin within 24 h. Moreover, only 28.27 ± 0.04% was retained on the epidermis. The developed ADA-LP gel showed significant improvement in the acne lesions in mice with no visible scars and inflammation on the skin. Therefore, ADA-LP-based gel could be a promising carrier system for the safe and effective delivery of Adapalene.
Ultrasound-Assisted Extraction of Mango (Mangifera indica) Kernel Starch: Chemical, Techno-Functional, and Pasting Properties
Authors: Mieles-Gómez Luis , Quintana Somaris E. , García-Zapateiro Luis A.
Published: February 2023

(1) Background: Starch is the main component of mango (Mangifera indica) kernel, making it an alternative to obtain an ingredient from a non-conventional source with potential application in food and other industrial applications; however, reports on the use of new extraction techniques for this material are scarce. The main objective of this research was to evaluate the effect of ultrasound-assisted extraction (UAE) on the yield, chemical, techno-functional, rheological, and pasting properties of starch isolated from a non-conventional source such as a mango kernel. (2) Methods: Different power sonication conditions (120, 300, and 480 W) and sonication time (10, 20, and 30 min) were evaluated along with a control treatment (extracted by the wet milling method). (3) Results: Ultrasound-assisted extraction increases starch yield, with the highest values (54%) at 480 W and 20 min. A significant increase in the amylose content, water-holding capacity, oil-holding capacity, solubility, and swelling power of ultrasonically extracted starches was observed. Similarly, mango kernel starch (MKS) exhibited interesting antioxidant properties. The sol-gel transition temperature and pasting parameters, such as the breakdown viscosity (BD) and the setback viscosity (SB), decreased with ultrasound application; (4) Conclusion: indicating that ultrasound caused changes in physical, chemical, techno-functional, rheological, and pasting properties, depending on the power and time of sonication, so it can be used as an alternative starch extraction and modification technique, for example, for potential application in thermally processed food products such as baked goods, canned foods, and frozen foods.
Chemokine Regulation in Temporomandibular Joint Disease: A Comprehensive Review
Authors: Qiao Yusen , Li Jun , Yuh Catherine , Ko Frank , Mercuri Louis G. , Alkhudari Jad , Pourzal Robin , Oh Chun-do
Published: February 2023

Temporomandibular joint disorders (TMDs) are conditions that affect the muscles of mastication and joints that connect the mandible to the base of the skull. Although TMJ disorders are associated with symptoms, the causes are not well proven. Chemokines play an important role in the pathogenesis of TMJ disease by promoting chemotaxis inflammatory cells to destroy the joint synovium, cartilage, subchondral bone, and other structures. Therefore, enhancing our understanding of chemokines is critical for developing appropriate treatment of TMJ. In this review, we discuss chemokines including MCP-1, MIP-1α, MIP-3a, RANTES, IL-8, SDF-1, and fractalkine that are known to be involved in TMJ diseases. In addition, we present novel findings that CCL2 is involved in β-catenin-mediated TMJ osteoarthritis (OA) and potential molecular targets for the development of effective therapies. The effects of common inflammatory factors, IL-1β and TNF-α, on chemotaxis are also described. In conclusion, this review aims to provide a theoretical basis for future chemokine-targeted therapies for TMJ OA.
Transient Receptor Potential Ankyrin 1 (TRPA1) Methylation and Chronic Pain: A Systematic Review
Authors: Celsi Fulvio , Peri Francesca , Cavasin Julia , Zupin Luisa , Cozzi Giorgio , Barbi Egidio , Crovella Sergio
Published: February 2023

Background and Objective: Chronic pain represents a major global health issue in terms of psycho-physiological, therapeutic, and economic burden, not limited to adults but also to the pediatric age. Despite its great impact, its molecular mechanisms have still not been completely unraveled. Focusing on the impact of epigenetics in the pain complex trait, we assessed the association between chronic pain and the methylation pattern of TRPA1, a key gene related to pain sensitivity. Methods: We conducted a systematic review retrieving articles from three different databases. After deduplication, 431 items were subjected to manual screening, and then 61 articles were selected and screened again. Of these, only six were maintained for meta-analysis and analyzed using specific R packages. Results: Six articles were divided into two groups (group 1: comparison of mean methylation levels between healthy subjects and patients with chronic pain; group 2: correlation between mean methylation levels and pain sensation). A non-significant mean difference was obtained from the analysis of group 1 with a value of 3.97 (95% C.I. −7.79; 15.73). Analysis of group 2 showed a high level of variability between studies (correlation = 0.35, 95% C.I. −0.12; 0.82) due to their heterogeneity (I2 = 97%, p < 0.01). Conclusions: Despite the high variability observed in the different studies analyzed, our results suggest that hypermethylation and increased pain sensitivity could be connected, possibly due to the variation of TRPA1 expression.
The Satellite DNAs Populating the Genome of Trigona hyalinata and the Sharing of a Highly Abundant satDNA in Trigona Genus
Authors: Pereira Jaqueline A. , Cabral-de-Mello Diogo C. , Lopes Denilce M.
Published: February 2023

Among Meliponini species, c-heterochromatin can occupy large portions of chromosomes. This characteristic could be useful for understanding evolutionary patterns of satellite DNAs (satDNAs), although few sequences have been characterized in these bees. In Trigona, phylogenetically represented by clades A and B, the c-heterochromatin is mostly located in one chromosome arm. Here we used different techniques, including restriction endonucleases and genome sequencing followed by chromosomal analysis, to identify satDNAs that may be contributing to the evolution of c-heterochromatin in Trigona. Our results revealed a highly abundant ThyaSat01-301 satDNA, corresponding to about 13.77% of the Trigona hyalinata genome. Another seven satDNAs were identified, one corresponding to 2.24%, and the other six corresponding to 0.545% of the genome. The satDNA ThyaSat01-301 was shown to be one of the main constituents of the c-heterochromatin of this species, as well as of other species belonging to clade B of Trigona. However, this satDNA was not observed on the chromosomes of species from clade A, demonstrating that the c-heterochromatin is evolving divergently between species of clade A and B, as a consequence of the evolution of repetitive DNA sequences. Finally, our data suggest the molecular diversification of the karyotypes, despite a conservated macrochromosomal structure on the genus.
Remote Sensing Image Scene Classification: Advances and Open Challenges
Authors: Tombe Ronald , Viriri Serestina
Published: February 2023

Deep learning approaches are gaining popularity in image feature analysis and in attaining state-of-the-art performances in scene classification of remote sensing imagery. This article presents a comprehensive review of the developments of various computer vision methods in remote sensing. There is currently an increase of remote sensing datasets with diverse scene semantics; this renders computer vision methods challenging to characterize the scene images for accurate scene classification effectively. This paper presents technology breakthroughs in deep learning and discusses their artificial intelligence open-source software implementation framework capabilities. Further, this paper discusses the open gaps/opportunities that need to be addressed by remote sensing communities.
Piuro Landslide: 3D Hydromechanical Numerical Modelling of the 1618 Event
Authors: Morcioni Andrea , Apuani Tiziana , Cecinato Francesco
Published: February 2023

The Piuro 1618 landslide represents a well-known case history of a large Alpine landslide. It destroyed the ancient village of Piuro (Italian Bregaglia Valley), renowned as an important trading center between the Mediterranean region and Northern Europe. The event had a significant impact among communities of all Alpine regions and was well documented by chronicles and paintings during subsequent decades. However, some aspects, such as the geometry reconstruction of the landslide body, the location of the landslide scarp, and its dynamics, remained undefined in previous studies, and a geomechanical characterization of the failure area is completely missing. Using field and laboratory analysis followed by stress–strain numerical modelling, this work develops a 3D conceptual geomechanical model of the slope considering its complex geological framework. The aim is to back-analyze the 1618 event, defining predisposing and triggering factors of the sliding event, and providing verifications on the geometry and location of the failure scar, as well as on the landslide dynamics. A coupled hydro-mechanical analysis with a 3D numerical approach is presented, assuming a rainfall scenario as a possible triggering factor. Simulated displacement and the development of a deep region of shear strain localization at a depth roughly corresponding to that of the detected Piuro sliding surface, allow us to highlight the mechanical role of geological elements outcropping along the slope and to validate the proposed scenario as a likely triggering factor for the 1618 event.
Relationship between NDVI of Patches and Cover Area of Grasses, Shrubs and Bare Soil Components of a Semi-Arid Steppe from North-West Patagonia, Argentina
Authors: Fariña Clara , Aramayo Valeria , Perri Daiana , Martín Albarracín Valeria , Umaña Fernando , Bruzzone Octavio Augusto , Easdale Marcos H.
Published: February 2023

Distinguishing the contributions of different vegetation cover such as shrubs and grasses components into the primary production in arid and semi-arid rangelands is a key step to understanding changes at a landscape scale. The aim was to assess the contribution of shrubs, grasses and bare soil components into a total biophysical variable at a patch level, and the relationship between that biophysical variable and remote sensing vegetation index, in a grass–shrub steppe from North-West Patagonia, Argentina. We conducted a field survey in the period 2015–2017 to analyzing the relationship between monthly values of Normalized Difference Vegetation Index (NDVI) of two grasses, two shrub species and bare soil, weighted by their cover area at a patch level, and the concomitant patch NDVI records, respectively. The contribution of the patch components to the total NDVI value at a patch level was additive. The relationship between the weighted NDVI of patch components and the concomitant NDVI value at a patch level along time was linear for perennial grasses and deciduous shrub–grass patches, but linearity was not significant for most perennial shrub–grass patches. Differences among patch compositions and their surface reflectance suggest the need to move forward in a more precise distinction of the floristic composition of patches, to better understanding their contribution to NDVI temporal dynamics at a landscape scale.
Non-Invasive Analyses of Italian “Ostrogothic” Jewellery: The Desana Treasure
Authors: Aceto Maurizio , Calà Elisa , Robotti Francesca , Gil Joan Pinar , Castronovo Simonetta , Gulmini Monica , Labate Maria , Agostino Angelo
Published: February 2023

The Desana treasure is a remarkable assemblage of items made of gold, silver, gemstones and glasses found in north-western Italy. Most scholars agree on the fact that the core of the treasure might have belonged to a single deposit resulted from a long period of selection, accumulation and use. The treasure testifies to the evolution of goldsmiths’ art in Ostrogothic Italy and represents an extraordinary material trace of the Italian elites of the 5th–6th centuries. The Desana treasure was investigated with non-invasive instrumental analytical techniques, namely optical microscopy, UV-visible diffuse reflectance spectrophotometry with optical fibres and X-ray fluorescence spectrometry in order to record the chemical features of gemstones, coloured glasses and precious metals employed to produce the items. As for the gemstones, besides identifying typologies, data suggested India as the source for a sapphire pendant and for most of the garnets, whereas the emeralds may belong to different sources, among which Pakistan, India and Egypt. The investigation revealed the colouring agents and compositional features of the glasses, and the composition of the gold alloys. The results of the investigation highlight that the raw materials used by Late Antique Italian goldsmiths did not differ significantly from other neighbouring European and Mediterranean regions, although the garnets show some differences if compared with coeval jewels recorded north of the Alps. The dataset produced in this work complements the stylistic approach for the study of these amazing traces of the past and deepens our knowledge on the role of the Italian “Ostrogothic” jewellery in the frame of the coeval Mediterranean, Central European and Northern Pontic metalwork traditions.
Geomagnetic and FDEM Methods in the Roman Archaeological Site of Bocca Delle Menate (Comacchio, Italy)
Authors: Rizzo Enzo , Dubbini Rachele , Clementi Jessica , Capozzoli Luigi , De Martino Gregory , Fornasari Giacomo , Fiano Francesca Romana , Lombardi Matteo
Published: February 2023

The increasing use of geophysical investigations for archaeological purposes is now provided also by Italian reforms about preventive archaeology. They allow not only the discovery or the spatial definition of possible buried archaeological evidence, but they are also able to define the state of preservation of ancient structures. The Bocca delle Menate archaeological site is in Comacchio village territory, situated in Ferrara provence (Emilia Romagna region, Italy). The archeological site provides important evidence of the Roman presence in the Po Delta (Italy). The Roman villa was excavated between 1958 and 1959, during the reclaiming works in the Mezzano Valley (Comacchio, Ferrara). An archaeological preliminary survey and a geophysical field trip using Geomagnetic and Frequency Domain Electromagnetic Methods were carried out, following the aim to identify the planimetry of the villa previously excavated and eventually newly discovered archaeological remains. The geomagnetic results detected the archaeological buried structures, even if the original disposition of them is not completely highlighted. The electromagnetic method was able to depict the geological and geomorphological background surrounding the Roman villa. The obtained results highlighted that the applied geophysical methods are excellent tools for the preservation, protection, and monitoring of archaeological heritage previously excavated, adding to their already known importance as best tools for new archaeological buried remains detections.
Gardens as Architectural Heritage of the Bastion Castle Ensemble
Authors: Tikhonova Olha
Published: February 2023

Historic gardens are often seen in the context of botanical heritage, which emphasizes their aesthetic and botanical value. Yet, their architectural importance as an integral part of the architectural heritage of the bastion castle ensemble is ignored. Finding the relationship between the castle and the garden is presently complicated by considerable changes in the townscape since the 17th century and the lack of details on these gardens’ depictions on maps. The paper demonstrates how historical maps, especially military ones combined with modern on-site analyses can reveal the main architectural types of the gardens in the bastion castle ensemble and show their most important architectural features that should be preserved. The results demonstrate three scenarios in the relationship between the garden and the palace component of bastion castle, their main features, and the challenges facing them. This elucidation of the main features can help to conserve and to valorize plans for the gardens in these ensembles.
Non-Thermal Plasma and Soilless Nutrient Solution Application: Effects on Nutrient Film Technique Lettuce Cultivation
Authors: Nicoletto Carlo , Falcioni Valentina , Locatelli Silvia , Sambo Paolo
Published: February 2023

Soilless cultivation is one of the best examples of the sustainable intensification concept due to the high use efficiency of its inputs. Managing the nutrient solution through a closed cycle system represents a crucial objective to be pursued, but the recovery of the nutrient solution requires chemical correction and sanitization. The application of non-thermal plasma (NTP) in agriculture promotes the safety of the nutrient solution, decreasing the use of chemicals. The aim of this study was to evaluate the effects of cold plasma on the nutrient depletion, yield, and qualitative traits of lettuce. A closed soilless system (nutrient film technique) was used to compare different NTP treatments: control, low ionization (LI), and high ionization (HI) in two successive lettuce cycles. No significant differences within the nutrient depletion trends were observed. The treated lettuce’s yield was 12% higher than that of the control, characterized by a higher total soluble solid content and a significantly higher electrical conductivity and titratable acidity than the control. The ion content was higher in HI plants, as were the contents of nitrogen, phosphorous, and potassium. In HI plants, the leaf pigments were higher, but no significant changes were observed for the antioxidant content. Cold plasma is a promising strategy that brings benefits to the crop.
Production of Quinoa Leafy Greens in High Tunnel for Season Extension in Missouri
Authors: Pathan Safiullah , Ndunguru Grato , Islam Md R. , Jhumur Sadia T. , Ayele Addissu G.
Published: February 2023

There is limited information regarding the use of quinoa fresh leaves as a vegetable. Thus, the objective of this study was to promote quinoa green leaves as a vegetable, as well as to conduct a season extension feasibility study using high tunnels. The study was conducted during the spring seasons of 2020, 2021, and 2022 at the George Washington Carver farm of Lincoln University in Jefferson City, MO, USA. Three quinoa genotypes were used in a randomized complete block design (RCBD) with three replications over three years. Agronomic data and leaf nutritional analyses for young plants approximately 30 days old were evaluated. Among the three quinoa genotypes, genotype Ames 13724 gave the highest yield of leafy greens and was consistent over the three years. Additionally, the use of high tunnels served as a season extension tool by accelerating the production of quality fruits and vegetables outside typical growing seasons. The results indicated that season extension of quinoa leafy greens production in a high tunnel is possible with a three-week earlier harvest in early spring when fewer fresh vegetables are available in the markets. Farmers can increase farm income by selling this specialty vegetable in the early season for premium prices.
Anatomical and Chemical Analysis of Moringa oleifera Stem Tissue Grown under Controlled Conditions
Authors: McVea Holly M. , Wood Lisa J.
Published: February 2023

Moringa oleifera is a relatively well-studied ethnobotanical species, but information is limited regarding its stem anatomy and the production potential of phytochemicals from bark tissue. Knowing that variation exists in the production of chemical defenses by plants with growing conditions and with developmental stages, M. oleifera was grown under controlled conditions to characterize stem tissues and to determine if stem bark contained the correct phytochemical compounds to be of value in medicinal treatments. We used microscopy to characterize the stem anatomy of M. oleifera and analyzed stem bark extracts using FTIR and GC to identify 4-(α-L-rhamnosyloxy)-benzyl isothiocyanate (moringin) and benzylamine (moringine) in tissue. We found the stems to be in transition between juvenile and mature stages of development at 4 months old under the growth conditions used. In 7-month-old stems, we found the presence of moringin in all bark samples and did not find any moringine. These results indicate that M. oleifera bark of 7-month-old trees grown in greenhouse conditions may be valuable for drug development.
Effect of Musical Stimulation on Placental Programming and Neurodevelopment Outcome of Preterm Infants: A Systematic Review
Authors: Pino Olimpia , Di Pietro Sofia , Poli Diana
Published: February 2023

Background: The fetal environment is modulated by the placenta, which integrates and transduces information from the maternal environment to the fetal developmental program and adapts rapidly to changes through epigenetic mechanisms that respond to internal (hereditary) and external (environmental and social) signals. Consequently, the fetus corrects the trajectory of own development. During the last trimester of gestation, plasticity shapes the fetal brain, and prematurity can alter the typical developmental trajectories. In this period, prevention through activity-inducing (e.g., music stimulation) interventions are currently tested. The purpose of this review is to describe the potentialities of music exposure on fetus, and on preterm newborns in the Neonatal Intensive Care Unit evaluating its influence on neurobehavioral development. Methods: Databases were searched from 2010 to 2022 for studies investigating mechanisms of placental epigenetic regulation and effects of music exposure on the fetus and pre-term neonates. Results: In this case, 28 selected papers were distributed into three research lines: studies on placental epigenetic regulation (13 papers), experimental studies of music stimulation on fetus or newborns (6 papers), and clinical studies on premature babies (9 papers). Placental epigenetic changes of the genes involved in the cortisol and serotonin response resulted associated with different neurobehavioral phenotypes in newborns. Prenatal music stimulation had positive effects on fetus, newborn, and pregnant mother while post-natal exposure affected the neurodevelopment of the preterm infants and parental interaction. Conclusions: The results testify the relevance of environmental stimuli for brain development during the pre- and perinatal periods and the beneficial effects of musical stimulation that can handle the fetal programming and the main neurobehavioral disorders.
Understanding Sexualized Drug Use: Substances, Reasons, Consequences, and Self-Perceptions among Men Who Have Sex with Other Men in Spain
Authors: Íncera-Fernández Daniel , Román Francisco J. , Moreno-Guillén Santiago , Gámez-Guadix Manuel
Published: February 2023

Sexualized drug use (SDU) has been identified as a health risk factor among gay, bisexual, and other men who have sex with men (GBMSM). This study aimed to analyze the associations between SDU frequency and a broad set of substances, motives, consequences, and self-perceptions. Sampling was conducted through an online survey. The final sample consisted of 185 GBMSM aged between 18 and 78 years old (mean age = 38.38, SD = 11.52) who engaged in SDU. We analyzed the frequency of SDU in terms of practicing it “once,” “moderately” (“once a month or less” or “a few times a month”), or “frequently” (from “once a week” to “daily”) during the previous 18 months. A questionnaire was administered through which sociodemographic variables, substances, reasons, consequences, and self-perceptions of SDU practice were analyzed. Participants who did so frequently were significantly more likely to use mephedrone, methamphetamine, and GHB/GBL than those who performed SDU less often (large effect sizes). In addition, habitual SDU was associated with motivations to achieve pleasurable emotions and sensations and manage negative feelings. Health implications, such as blackout moments, were also significantly related to frequent SDUs. Finally, those who practiced frequent SDU perceived it as a severe problem and wanted to control it. These data indicate the importance of raising awareness of chemsex as a public health problem among GBMSM. Specific identification, education, and prevention programs need to be strengthened to reduce the incidence of the most undesirable implications of SDU among GBMSM.
Contributing Factors to the Burden on Primary Family Caregivers of Stroke Survivors in South Korea
Authors: Kwon Bo Mi , Lee Hyun Haeng , Sohn Min Kyun , Kim Deog Young , Shin Yong-Il , Oh Gyung-Jae , Lee Yang-Soo , Joo Min Cheol , Lee So Young , Song Min-Keun , Han Junhee , Ahn Jeonghoon , Chang Won Hyuk , Lee Jongmin , Kim Yun-Hee
Published: February 2023

The purpose of this study is to identify the factors associated with the burden on primary family caregivers of stroke patients at home without care services. For this study, the Korean Stroke Cohort for Functioning and Rehabilitation (KOSCO) data were used. Of the total 8010 caregivers, 1133 family caregiver burden was assessed with the shortened Caregiver Burden Inventory (CBI) 3 months after stroke. Patient and caregiver-related factors affecting the heavier burden of caregivers were identified by comparing the heavier caregiver burden group and the lighter caregiver burden group, which divided according to the CBI scores. The 719 (63.5%) family caregiver cared for patients at home without care services. Logistic regression analysis showed that four or more comorbidities (p = 0.002), neurological impairment at early onset (p < 0.001), dependence on daily life (p < 0.001), aphasia (p = 0.024), and depression(p < 0.001) were associated with a heavier burden of care. According to the shortened CBI, caregivers tended to be concerned more about psychological stress than physical strain. The findings suggest the importance of proactively guiding the emotional support services to caregivers who are at high risk of the heavier burden of patient care.
Degradation Characteristics and Remediation Ability of Contaminated Soils by Using β-HCH Degrading Bacteria
Authors: Chen Qing , Shi Huijun , Liang Yanpeng , Qin Litang , Zeng Honghu , Song Xiaohong
Published: February 2023

Three degradation strains that can utilize β-Hexachlorocyclohexanes (β-HCH) as the sole carbon source were isolated from the soil substrate of constructed wetland under long-term β-HCH stress, and they were named A1, J1, and M1. Strains A1 and M1 were identified as Ochrobactrum sp. and strain J1 was identified as Microbacterium oxydans sp. by 16S rRNA gene sequence analysis. The optimum conditions for degradation with these three strains, A1, J1, and M1, were pH = 7, 30 °C, and 5% inoculum amount, and the degradation rates of 50 μg/L β-HCH under these conditions were 58.33%, 51.96%, and 50.28%, respectively. Degradation characteristics experiments showed that root exudates could increase the degradation effects of A1 and M1 on β-HCH by 6.95% and 5.82%, respectively. In addition, the degradation bacteria A1 and J1 mixed in a ratio of 1:1 had the highest degradation rate of β-HCH, which was 69.57%. An experiment on simulated soil remediation showed that the compound bacteria AJ had the best effect on promoting the degradation of β-HCH in soil within 98 d, and the degradation rate of β-HCH in soil without root exudates was 60.22%, whereas it reached 75.02% in the presence of root exudates. The addition of degradation bacteria or degradation bacteria-root exudates during soil remediation led to dramatic changes in the community structure of the soil microorganisms, as well as a significant increase in the proportion of aerobic and Gram-negative bacterial groups. This study can enrich the resources of β-HCH degrading strains and provided a theoretical basis for the on-site engineering treatment of β-HCH contamination.
Effects of Plant-Emitted Monoterpenes on Anxiety Symptoms: A Propensity-Matched Observational Cohort Study
Authors: Donelli Davide , Meneguzzo Francesco , Antonelli Michele , Ardissino Diego , Niccoli Giampaolo , Gronchi Giorgio , Baraldi Rita , Neri Luisa , Zabini Federica
Published: February 2023

Immersive experiences in green areas, particularly in forests, have long been known to produce beneficial effects for human health. However, the exact determinants and mechanisms leading to healthy outcomes remain to be elucidated. The purpose of this observational cohort study was to investigate whether inhaling plant-emitted biogenic volatile compounds, namely monoterpenes (MTs), can produce specific effects on anxiety symptoms. Data from 505 subjects participating in 39 structured forest therapy sessions at different Italian sites were collected. The air concentration of monoterpenes was measured at each site. STAI state questionnaires were administered before and after the sessions as a measure of anxiety. A propensity score matching analysis was then performed, considering an above-average exposure to inhalable air MTs as the treatment. The estimated effect was −1.28 STAI-S points (95% C.I. −2.51 to −0.06, p = 0.04), indicating that the average effect of exposure to high MT air concentrations during forest therapy sessions was to decrease anxiety symptoms.
Spatial and Temporal Analyses of the Event of Death for 1480 in Milan Using the Data Contained in the Sforza’s Registers of the Dead
Authors: Luconi Ester , Boracchi Patrizia , Nodari Riccardo , Comandatore Francesco , Marano Giuseppe , Vaglienti Folco , Galli Massimo , Biganzoli Elia
Published: February 2023

Historical death registration was conducted primarily to assess the presence of plague. The Liber Mortuorum of Milan was one of Europe’s first registers with many socio-demographical details. In this work, we consider 1480 to make spatial and temporal analyses of the event of death to find possible explanations about the events’ distribution and the events’ trend over time. The spatial analyses involved Moran’s I, the LISA, and the heatmaps; the temporal analysis applied the Durbin-Watson test. All the analyses were conducted separately on all subjects (1813), children (765), and adults (1046). Contrade (districts) were considered for spatial analysis. Moran’s I and the Durbin Watson test were significant on all subjects and children’s analyses, and the LISA showed the same results for those groups. Children may significantly impact the distribution of death and the trend over time. At least half of the children were 0 years old, and survival in the very first childhood period was closely linked to the family, so that it could be a proxy of the conditions of an area.
Continuous Exposure to Terrorism during the COVID-19 Pandemic: A Moderated Mediation Model in the Israeli Context
Authors: Leshem Becky , Kashy-Rosenbaum Gabriela , Schiff Miriam , Benbenishty Rami , Pat-Horenczyk Ruth
Published: February 2023

This study tested the role of perceived social support as a moderating factor in the mediation of COVID-19-related concerns in the association between continuous traumatic stress (CTS) and depression. The study participants were 499 college students who responded to an anonymous online questionnaire. Measures included the assessment of prior continuous exposure to threats of terrorism, COVID-19-related distress, perceived social support and depressive symptoms. The results demonstrated that COVID-19-related concerns mediated the relationship between continuous exposure to threats of terrorism and depression symptoms, and that perceived social support moderated the association between COVID-19-related concerns and depression. The implications of the study highlight the role of prior exposure to traumatic stress as a risk factor for depression and the role of social support as a protective factor. These results point to the need to develop accessible and non-stigmatic mental health services for populations exposed to other types of continuous traumatic stress.
Stress and Resilience Experiences during the Transition to Parenthood among Belgian Lesbian Mothers through Donor Insemination
Authors: D’Amore Salvatore , Maurisse Alexandre , Gubello Alessio , Carone Nicola
Published: February 2023

The present research explored parenting, stress, and resilience experiences among 16 Belgian, lesbian, first-time parental couples with donor-conceived children aged 3–72 months. In each couple, both mothers participated in a conjoint, semi-structured interview focused on their parenthood desire; the impact of stigmatization and social support from families of origin, friends, and institutions; and couple and family resources. Interviews were audio recorded, transcribed, and analyzed using Braun and Clarke’s reflective thematic analysis. Four themes were identified: (1) “The precious baby”: Realizing the parenthood project; (2) “Can we show ourselves in public without prying eyes?”: Family social visibility; (3) “It’s complicated!”: Parental legal recognition and role imbalance; and (4) “How can we handle this?”: Family resilience. The themes indicated that the child’s donor conception, the parents’ coming out, the non-gestational mother’s role, the legal obstacles encountered, and the need to find a balance between the two mothers in childcare tasks generated stress and required mothers to develop resilience strategies. The results suggest several potential areas for mental health practitioners to explore in clinical contexts when supporting intended lesbian mothers in their transition to parenthood through donor insemination.