bluuebunny/crossref_metadata_2025 huggingface dataset:
Targeted Isolation of Two New Anti-inflammatory and UV-A Protective Dipyrroloquinones from the Sponge-associated Fungus Aspergillus tamarii MCCF102
Authors: Niveditha Lekshmi , Fu Peng , Leao Tiago F. , Li Te , Wang Tingting , Poulin Remington X. , Gaspar Lorena R. , Naman C. Benjamin , Thavarool Puthiyedathu Sajeevan
Published: February 2022

In following up on observed in vitro anti-inflammatory activity of the organic extract of the marine sponge-derived fungus Aspergillus tamarii MCCF102, two new
dipyrrolobenzoquinones, terreusinone B and C (1 and 2), were discovered along with the known analogue, terreusinone (3). The structures of 1–3 were
determined by spectroscopic and spectrometric analyses, along with chemical inter-conversion. In vitro testing on lipopolysaccharide (LPS) stimulated RAW 264.7 murine macrophage cells
revealed that 1–3 exhibit anti-inflammatory activity by inhibiting nitric oxide production in a dose-dependent manner (IC50 < 1 µM) without any cytotoxicity
observed at the same concentrations. Due to this and the UV-A absorptive properties imparted by the highly conjugated structures of these molecules, the potential for using 1–3
or related analogues as natural sunscreen components is suggested. Gene sequencing and informatics biosynthetic gene cluster comparisons were insufficient to confidently elucidate the
biosynthetic origins of these compounds, possibly suggesting the occurrence of a gene cluster not detected in the initial sequencing or a non-canonical pathway that should be further
investigated.
Can Fecal Calprotectin Reflect Your Colonic Status?
Authors: Salama Rasha Ibrahim , Ahmed Mohammed Hussien
Published: February 2022

Background Organic colonic manifestation may be difficult to be differentiated from functional one. Inflammatory bowel disease (IBD) is a common chronic inflammatory and destructive disease of the bowel wall. Chronic inflammation is associated with ulcerations, strictures, perforations, and it is a risk factor for dysplasia and cancer. To reduce these long-standing complications, IBD patients are in a continuous need for early diagnosis1. Markers, such as erythrocyte sedimentation rate (ESR), and c-reactive protein (CRP), fecal calprotectin (FC) have been widely used as noninvasive parameters for IBD monitoring. We aimed, in this current study, to evaluate the value of fecal calprotectin and other noninvasive biomarkers in predicting abnormal histologic findings in patients undergoing colonoscopy.in addition to determine the cutoff value which predict IBD2.

Methods The present prospective study included 160 patients with complaint of colicky abdominal pain with frequent diarrhea associated with mucous and infrequent bleeding per rectum for more than 6 months. They presented partial improvement with medication and recurrence once stopping the treatment These patients had been recently diagnosed with IBD at many primary healthcare centers covering the areas of the Kafrelsheikh and Zagazik governorate in the North of Egyptian Nile delta. After complete history, clinical examination, and laboratory investigation, they were referred to the IBD clinic at Kafrelsheikh University Hospital for assessment and ileocolonoscopy with biopsies.

Results There was a wide spectrum of age of the studied patients, with mean age 40.12 ± 7.88 (minimum 18 and maximum 56 years). Regarding gender, males represented 87.5% of the studied patients. Forty percent of the patients with colonic manifestation were smokers, 57% preferred a spicy diet, and the majority had low educational level (77.5%). Forty percent had obvious blood in stool, 55% had occult blood, and raised ESR CRP occurred in 32.5% and 50%, respectively. Fecal calprotectin cutoff was > 159, with sensitivity 92.8% and specificity 97.5%.

Conclusions: Biomarkers (FC, ESR, CRP) can be used as noninvasive parameters for the early diagnosis and prediction of organic colonic disease. Fecal calprotectin in the IBD group revealed significant area under the curve (AUC) values and cutoff > 159, with sensitivity 92.8% and specificity 97.5%.
Outcomes Following Surgical Lung Biopsy for Interstitial Lung Diseases: A Monocenter Experience
Authors: Millaire Émilie , Ouellet Étienne , Provencher Steeve , Dion Geneviève , Fortin Marc , Martel Simon , Milot Julie , Bilodeau Lara , Conti Massimo
Published: February 2022

Background Surgical lung biopsy (SLB) is considered in the investigation of interstitial lung diseases (ILDs) when a complete clinical evaluation and a multidisciplinary discussion (MDD) do not allow the clinician to make a confident diagnosis. Owing to the risk of the procedure, an appropriate assessment of the risk/benefit ratio prior to the intervention is recommended. We aimed to assess the postoperative outcomes and diagnostic yield of SLB for the investigation of ILD in a tertiary care institution.

Methods We conducted a retrospective cohort study of consecutive subjects who underwent a SLB for the investigation of ILD in our center from 2009 to 2020. The postoperative mortality and complications rates as well as the diagnostic yield of the procedure were assessed.

Results Of the 1,805 patients newly investigated for ILD in our center from 2009 to 2020, 71 (3.93%) underwent a SLB. At days 30 and 90, the mortality rates were 0 and 2.8%, whereas 4.3 and 7.6% patients experienced an acute ILD exacerbation, respectively. In addition, 4 (5.8%) patients experienced infectious complications and 5 (7.0%) presented prolonged air leaks (all within 30 days). A definite pathological diagnosis was made in 47 (66.2%) patients. Following postoperative MDD, a confident diagnosis was made in 61 patients (85.9%) and resulted in a change of therapy in 49 (69.0%) patients.

Conclusion SLB for the diagnosis of unclassifiable ILDs is associated with low mortality but significant morbidity. However, it results in a confident diagnosis and a change in therapy in the majority of patients.
An Exploratory Analysis of the Internal Structure of Test Through a Multimethods Exploratory Approach of the ASQ:SE in Brazil
Authors: Anunciação Luis , Squires Jane , Landeira-Fernandez J. , Singh Ajay
Published: February 2022

Background A wide range of exploratory methods is available in psychometrics as means of gathering insight on existing data and on the process of establishing the number and nature of an internal structure factor of a test. Exploratory factor analysis (EFA) and principal component analysis (PCA) remain well-established techniques despite their different theoretical perspectives. Network analysis (NA) has recently gained popularity together with such algorithms as the Next Eigenvalue Sufficiency Test. These analyses link statistics and psychology, but their results tend to vary, leading to an open methodological debate on statistical assumptions of psychometric analyses and the extent to which results that are generated with these analyses align with the theoretical basis that underlies an instrument. The current study uses a previously published data set from the Ages & Stages Questionnaires: Social-Emotional to explore, show, and discuss several exploratory analyses of its internal structure. To a lesser degree, this study furthers the ongoing debate on the interface between theoretical and methodological perspectives in psychometrics.

Methods From a sample of 22,331 sixty-month-old children, 500 participants were randomly selected. Pearson and polychoric correlation matrices were compared and used as inputs in the psychometric analyses. The number of factors was determined via well-known rules of thumb, including the parallel analysis and the Hull method. Multidimensional solutions were rotated via oblique methods. R and Factor software were used, the codes for which are publicly available at https://luisfca.shinyapps.io/psychometrics_asq_se/.

Results Solutions from one to eight dimensions were suggested. Polychoric correlation overcame Pearson correlation, but nonconvergence issues were detected. The Hull method achieved a unidimensional structure. PCA and EFA achieved similar results. Conversely, six clusters were suggested via NA.

Conclusion The statistical outcomes for determining the factor structure of an assessment diverged, varying from one to eight domains, which allowed for different interpretations of the results. Methodological implications are further discussed.
Technical Considerations in Primary Repair of a Congenital Prostatic Rectourethral Fistula in an Adult-Sized Patient
Authors: Tirrell Timothy F. , Demehri Farokh R. , Nandivada Prathima , McNamara Erin R. , Dickie Belinda Hsi
Published: February 2022

Congenital anorectal malformations are generally diagnosed and repaired as a neonate or infant, but repair is sometimes delayed. Considerations for operative repair change as the patient approaches full stature. We recently encountered a 17-year-old male with an unrepaired congenital rectourethral fistula and detail our experience with his repair.

We elected to utilize a combined abdominal and perineal approach, with robotic assistance for division of his rectourethral fistula and pullthrough anoplasty. Cystoscopy was used simultaneously to assure full dissection of the fistula and to minimize the risk of leaving a remnant of the original fistula (also known as a posterior urethral diverticulum).

The procedure was well tolerated without complications. His anoplasty was evaluated 60 days postoperatively and was well healed without stricture. At 9 months of follow-up, he has good fecal and urinary continence.

Robotic assistance in this procedure allowed minimal perineal dissection while ensuring precise rectourethral fistula dissection. The length of the intramural segment of the fistula was longer than anticipated.

Simultaneous cystoscopy, in conjunction with the integrated robotic fluorescence system, helped reduce the risk of leaving a remnant of the original fistula.
Characterization of Acute Traumatic Coagulopathy in Cats and Association with Clinicopathological Parameters at Presentation
Authors: Gustin Ursina , Sigrist Nadja E. , Muri Benjamin M. , Spring Irina , Jud Schefer Rahel
Published: February 2022

Objective This study aimed to document rotational thromboelastometric (ROTEM) characteristics of traumatized cats and to investigate associations between clinicopathological parameters and acute traumatic coagulopathy (ATC). A secondary goal was to determine the relevance of autoheparinization in injured cats.

Study Design Cats presenting with acute (<12 hours) trauma were eligible. Cats were allocated to the ATC group (≥2 hypocoagulable parameters) or non-ATC group (≤1 hypocoagulable parameter) based on ROTEM analysis. Clinicopathological parameters were compared between groups and regression was used to find variables associated with ATC. Heparinase-modified ROTEM (HepTEM) was used to assess for heparin effects in a subgroup.

Results Fifty-three cats were included, and the incidence of ATC was 15%. Prolongation of both intrinsic and extrinsic clotting times (CT) was the most frequently altered ROTEM variable in the ATC group, but CTInTEM-prolongation also occurred in 47% of non-ATC cats. The incidence of autoheparinization, defined as concurrent CTInTEM prolongation and CTInTEM:HepTEM ratio >1.1, was 41% and was observed in both cats with and without ATC. None of the evaluated clinicopathological parameters were different between groups or associated with ATC.

Conclusion Acute traumatic coagulopathy in cats is mainly characterized by prolonged CT. No relationship between clinicopathological variables and ATC was identified and prediction of ATC based on these variables was not possible. While autoheparinization is important in cats, it is not the sole cause for ATC.
Relationship between Hemoglobin Concentration at Admission with the Incidence of No-Reflow Phenomenon and In-Hospital Mortality in Acute Myocardial Infarction with Elevation of ST Segments in Patients who underwent Primary Percutaneous Coronary Intervention
Authors: Akbar Kiagus Muhammad Andri , Dharma Surya , Andriantoro Hananto , Sukmawan Renan , Mangkuanom Arwin Saleh , Rejeki Vidya Gilang
Published: February 2022

Anemia in acute ST-segment elevation myocardial infarction (STEMI) is associated with a pro-coagulant state, contributing to the incidence of no-reflow phenomenon and increased mortality following primary percutaneous coronary intervention (PPCI). However, clinical data remain contradictory. The objective of our study was to evaluate the association of admission hemoglobin (Hb) concentration and in-hospital mortality of STEMI patients' post-PPCI, as well as final thrombolysis in myocardial infarction (TIMI) flow. A cross-sectional study was performed from the database of Jakarta Acute Coronary Syndrome Registry, consisting of 3,071 STEMI patients who underwent PPCI between January 2014 and December 2019. No-reflow phenomenon was defined as final TIMI flow <3 of the infarct-related artery. Outcome measures were the occurrence of no-reflow and in-hospital mortality. Anemia criteria were based on the World Health Organization. Anemia was found in 550 patients (17.9%). Patients with anemia were older (60 ± 10 years, p < 0.001), predominantly women (20.7 vs. 11.2%, p < 0.001), TIMI risk score >4 (45.8 vs. 30.4%, p < 0.00), and Killip classification >1 (25.8 vs. 20.8%, p < 0.009). Anemia at admission was not associated with no-reflow phenomenon (odds ratio [OR] = 0.889; 95% confidence interval [CI] = 0.654–1.209, p = 0.455). Multivariate regression models showed that anemia was not associated with in-hospital mortality (OR = 0.963; 95% CI = 0.635–1.459, p = 0.857) and with no-reflow phenomenon (OR = 0.939; 95% CI = 0.361–2.437, p = 0.896). Anemia upon admission was not related to the no-reflow phenomena or in-hospital mortality in STEMI patients undergoing PPCI.
Serum Long-Noncoding RNA H19 and β-Catenin as Biomarkers for Early Diagnosis of Colorectal Cancer in Egyptian Patients: A Case Control Study
Authors: Elshazly Ayat Abdelrahman Abdelrahman , Desouky Mohammed Nageeb , Diab Iman Hassan , Ibrahim Abeer Mahmoud Ali , Dwedar Fatma Ibrahim
Published: February 2022

Colorectal cancer (CRC) is the third most prevalent cancer and the second most common cause of cancer death; however, its early detection can improve the survival. Colonic polyps are considered one of the CRC's major risk factors. Throughout many biological processes and malignancies, the non-coding RNAs have essential functions. Certain long noncoding RNAs (lncRNAs), including H19, were supposed to be CRC possible biomarkers. Also, H19 has been reported to play a role in regulating the activity of β-catenin, a protein that regulates cell-to-cell adhesion, as well as gene transcription. The current work aimed to investigate the potential significance of LncRNA H19 relative serum expression level by quantitative polymerase chain reaction (q-PCR) and β-catenin by enzyme-linked immunosorbent assay (ELISA) as noninvasive biomarkers to discriminate between colorectal cancer and colonic polyps. The statistical analysis of the studied factors revealed that the serum expression of H19 and β-catenin in cancer cases were substantially greater than colonic polyp cases and normal control.

Conclusion The relative expressions of H19 and beta-catenin in the serum can significantly discriminate patients with CRC from those with polyp and normal controls, which could help when screening for CRC.
Traditional Japanese apricot (Prunus mume) induces osteocalcin in osteoblasts
Authors: Nomura Sachiko , Kono Ryohei , Imaoka Masakazu , Tazaki Fumie , Okuno Yoshiharu , Utsunomiya Hirotoshi , Takeda Masatoshi , Nakamura Misa
Published: February 2022

The fruit of Prunus mume (ume, also known as Japanese apricot) has been used as a functional food in Japan since ancient times. We previously reported that ume stimulates the differentiation of preosteoblastic cells. Osteocalcin (OCN) is secreted by osteoblasts, and there is known association with glucolipid metabolism and cognitive function. This study sought to clarify the relationship between ume extracts and OCN production both in vitro and in vivo. Alkaline phosphatase activity and OCN level in the ethyl acetate extracts of ume-treated extracts were significantly increased in preosteoblast MC3T3-E1 cells compared with the control group. In human study, serum OCN level was significantly higher in the high ume intake group than in the low intake group in community-dwelling participants over 60 years old. These results suggest that ume has the potential to upregulated OCN production both in vitro and in vivo.
PGS-server: accuracy, robustness and transferability of polygenic score methods for biobank scale studies
Authors: Yang Sheng , Zhou Xiang
Published: February 2022

Polygenic scores (PGS) are important tools for carrying out genetic prediction of common diseases and disease related complex traits, facilitating the development of precision medicine. Unfortunately, despite the critical importance of PGS and the vast number of PGS methods recently developed, few comprehensive comparison studies have been performed to evaluate the effectiveness of PGS methods. To fill this critical knowledge gap, we performed a comprehensive comparison study on 12 different PGS methods through internal evaluations on 25 quantitative and 25 binary traits within the UK Biobank with sample sizes ranging from 147 408 to 336 573, and through external evaluations via 25 cross-study and 112 cross-ancestry analyses on summary statistics from multiple genome-wide association studies with sample sizes ranging from 1415 to 329 345. We evaluate the prediction accuracy, computational scalability, as well as robustness and transferability of different PGS methods across datasets and/or genetic ancestries, providing important guidelines for practitioners in choosing PGS methods. Besides method comparison, we present a simple aggregation strategy that combines multiple PGS from different methods to take advantage of their distinct benefits to achieve stable and superior prediction performance. To facilitate future applications of PGS, we also develop a PGS webserver (http://www.pgs-server.com/) that allows users to upload summary statistics and choose different PGS methods to fit the data directly. We hope that our results, method and webserver will facilitate the routine application of PGS across different research areas.
Urinary biomarkers for the detection of ovarian cancer: a systematic review
Authors: Owens Gemma L , Barr Chloe E , White Holly , Njoku Kelechi , Crosbie Emma J
Published: February 2022

Currently, the only definitive method for diagnosing ovarian cancer involves histological examination of tissue obtained at time of surgery or by invasive biopsy. Blood has traditionally been the biofluid of choice in ovarian cancer biomarker discovery; however, there has been a growing interest in exploring urinary biomarkers, particularly as it is non-invasive. In this systematic review, we present the diagnostic accuracy of urinary biomarker candidates for the detection of ovarian cancer. A comprehensive literature search was performed using the MEDLINE/PubMed and EMBASE, up to 1 April 2021. All included studies reported the diagnostic accuracy using sensitivity and/or specificity and/or receiver operating characteristics (ROC) curve. Risk of bias and applicability of included studies were assessed using the QUADAS-2 tool. Twenty-seven studies were included in the narrative synthesis. Protein/peptide biomarkers were most commonly described (n = 18), with seven studies reporting composite scores of multiple protein-based targets. The most frequently described urinary protein biomarker was HE4 (n = 5), with three studies reporting a sensitivity and specificity > 80%. Epigenetic (n = 1) and metabolomic/organic compound biomarkers (n = 8) were less commonly described. Overall, six studies achieved a sensitivity and specificity of >90% and/or an AUC > 0.9. Evaluation of urinary biomarkers for the detection of ovarian cancer is a dynamic and growing field. Currently, the most promising biomarkers are those that interrogate metabolomic pathways and organic compounds, or quantify multiple proteins. Such biomarkers require external validation in large, prospective observational studies before they can be implemented into clinical practice.
Developmental trajectory of MEG resting-state oscillatory activity in children and adolescents: a longitudinal reliability study
Authors: Candelaria-Cook Felicha T , Solis Isabel , Schendel Megan E , Wang Yu-Ping , Wilson Tony W , Calhoun Vince D , Stephen Julia M
Published: February 2022

Neural oscillations may be sensitive to aspects of brain maturation such as myelination and synaptic density changes. Better characterization of developmental trajectories and reliability is necessary for understanding typical and atypical neurodevelopment. Here, we examined reliability in 110 typically developing children and adolescents (aged 9–17 years) across 2.25 years. From 10 min of magnetoencephalography resting-state data, normalized source spectral power and intraclass correlation coefficients were calculated. We found sex-specific differences in global normalized power, with males showing age-related decreases in delta and theta, along with age-related increases in beta and gamma. Females had fewer significant age-related changes. Structural magnetic resonance imaging revealed that males had more total gray, subcortical gray, and cortical white matter volume. There were significant age-related changes in total gray matter volume with sex-specific and frequency-specific correlations to normalized power. In males, increased total gray matter volume correlated with increased theta and alpha, along with decreased gamma. Split-half reliability was excellent in all frequency bands and source regions. Test–retest reliability ranged from good (alpha) to fair (theta) to poor (remaining bands). While resting-state neural oscillations can have fingerprint-like quality in adults, we show here that neural oscillations continue to evolve in children and adolescents due to brain maturation and neurodevelopmental change.
“A Stern Language of Beauty”: Ecological Grief and Ecofeminist Ethics in Paula Meehan’s Poetry
Authors: Du Jun
Published: February 2022

Through publicly mourning the loss of family members, communities, and natural habitats, Paula Meehan’s poems de-privatize and politicize grieving and lamentation, functioning in resistance to the existing social and cultural norms. From Reading the Sky (1985) to As If By Magic (2020), Meehan has been conscious of and has continuously delineated the deterioration of the ecosystem due to the exploitative expansion of human society. Drawing on ecofeminist theories and Judith Butler’s theorizations of precariousness and grievability, the essay explores how Meehan depicts ecological grief and the interrelationship between human and nonhuman beings at different stages of her poetic career; it further probes how she illuminates and complicates the ethics of mourning and ecofeminism in her ecological elegies.
Antibiotic susceptibility of Bacillus velezensis
Authors: Heo Ganghun , Kong Haram , Kim Namwon , Lee Sugyeong , Sul Sooyoung , Jeong Do-Won , Lee Jong-Hoon
Published: February 2022

We evaluated the antibiotic minimum inhibitory concentrations (MICs) of 123 Bacillus velezensis strains predominantly isolated from fermented soybean foods from Korea. When the 2018 European Food Safety Authority breakpoint values for Bacillus spp. were applied, all the strains were sensitive to chloramphenicol, clindamycin, erythromycin, gentamicin, kanamycin, tetracycline and vancomycin, and eight strains (6.5%) were resistant to streptomycin. The population distribution in MIC tests with streptomycin was continuous and the profile was clearly different from that expected for acquired antibiotic resistance. As of 25 October 2021, there were 181 complete published genomes of B. velezensis strains; 175 (96.7%) and 136 (75.2%) of these strains, respectively, possess potential tetracycline and streptomycin resistance genes tetL and ant(6) in the chromosome. In Bacillus licheniformis, SpeG confers resistance to clindamycin and there is an ‘speG’ gene annotated in the genomes of 180 B. velezensis strains; however, the gene products exhibit ≤26.6% amino acid identity with that from B. licheniformis DSM 13T. All the potential antibiotic resistance genes in the 181 B. velezensis strains were intrinsic, and traits of lateral gene transfer were not found. In this context, B. velezensis may not present a high risk in terms of antibiotic resistance in food fermentation or human use.
Synergistic effects of plant hormones on spontaneous late-ripening mutant of ‘Jinghong’ peach detected by transcriptome analysis
Authors: Zhang Man , Du Tingting , Yin Yarui , Cao Hongyan , Song Zhihua , Ye Mao , Liu Yating , Shen Yanhong , Zhang Libin , Yang Qing , Meng Dong , Wu Junkai
Published: February 2022

Objectives

Peach (Prunus persica L.) is an ancient fruit tree that originated from China. It is the climacteric fruit belonging to genus Prunus in family Rosaceae. Ethylene, which is produced during ripening, accelerates fruit softening, and therefore peaches cannot be stored for a long time.

Materials and Methods

To study the mechanism of fruit late ripening, transcriptome analysis of the fruit of a late-ripening mutant of ‘Jinghong’ peach was performed to identify genes and pathways involved in fruit late ripening.

Results

A total of 1805, 1511, and 2309 genes were found to be differentially expressed in W2_vs_M1, W3_vs_M2, and W3_vs_M3, respectively. Functional enrichment analysis of the differentially expressed genes showed they were related to carotenoid biosynthesis, starch and sucrose metabolism plant hormone signal transduction, flavonoid biosynthesis, and photosynthesis. The expression trends of ripening-related genes that encode transcription factors and plant hormone signal transduction-related genes that encode enzymes were similar.

Conclusions

It will help to elucidate the transcriptional regulatory network of fruit development in the spontaneous late-ripening mutant of ‘Jinghong’ peach and provide a theoretical basis for understanding the molecular regulatory mechanism of fruit ripening.
Genetic mapping of loci involved in oil tocopherol composition control in Russian sunflower (Helianthus annuus L.) lines
Authors: Gubaev Rim , Boldyrev Stepan , Martynova Elena , Chernova Alina , Kovalenko Tatyana , Peretyagina Tatyana , Goryunova Svetlana , Goryunov Denis , Mukhina Zhanna , Ben Cecile , Gentzbittel Laurent , Khaitovich Philipp , Demurin Yakov
Published: February 2022

Tocopherols are antioxidants that preserve oil lipids against oxidation and serve as a natural source of vitamin E in the human diet. Compared with other major oilseeds like rapeseed and soybean, sunflower (Helianthus annuus L.) exhibits low phenotypic diversity of tocopherol composition, both in wild and cultivated accessions from germplasm collections. Two major mutations that alter tocopherol composition were identified in genetic collections, and several studies suggested additional loci controlling tocopherol composition, with their expression possibly depending on the genetic background. In the present study, we performed QTL mapping of tocopherol composition in two independent F2 crosses between lines with contrasting tocopherol composition from the Pustovoit All-Russia Research Institute of Oil Crops (VNIIMK) collection. We used genotyping-bysequencing (GBS) to construct single nucleotide polymorphism-based genetic maps, and performed QTL mapping using quantitative and qualitative encoding for phenotypic traits. Our results support the notion that the tocopherol composition in the assessed crosses is controlled by two loci. We additionally selected and validated two single nucleotide polymorphism markers for each cross which could be used for marker-assisted selection.
Improving genomic predictions with inbreeding and nonadditive effects in two admixed maize hybrid populations in single and multienvironment contexts
Authors: Roth Morgane , Beugnot Aurélien , Mary-Huard Tristan , Moreau Laurence , Charcosset Alain , Fiévet Julie B
Published: February 2022

Genetic admixture, resulting from the recombination between structural groups, is frequently encountered in breeding populations. In hybrid breeding, crossing admixed lines can generate substantial nonadditive genetic variance and contrasted levels of inbreeding which can impact trait variation. This study aimed at testing recent methodological developments for the modeling of inbreeding and nonadditive effects in order to increase prediction accuracy in admixed populations. Using two maize (Zea mays L.) populations of hybrids admixed between dent and flint heterotic groups, we compared a suite of five genomic prediction models incorporating (or not) parameters accounting for inbreeding and nonadditive effects with the natural and orthogonal interaction approach in single and multienvironment contexts. In both populations, variance decompositions showed the strong impact of inbreeding on plant yield, height, and flowering time which was supported by the superiority of prediction models incorporating this effect (+0.038 in predictive ability for mean yield). In most cases dominance variance was reduced when inbreeding was accounted for. The model including additivity, dominance, epistasis, and inbreeding effects appeared to be the most robust for prediction across traits and populations (+0.054 in predictive ability for mean yield). In a multienvironment context, we found that the inclusion of nonadditive and inbreeding effects was advantageous when predicting hybrids not yet observed in any environment. Overall, comparing variance decompositions was helpful to guide model selection for genomic prediction. Finally, we recommend the use of models including inbreeding and nonadditive parameters following the natural and orthogonal interaction approach to increase prediction accuracy in admixed populations.
Interpersonal Discussion and Political Knowledge: Unpacking the Black Box via a Combined Experimental and Content-Analytic Approach
Authors: Moore Ryan C , Coronel Jason C
Published: February 2022

Over 130 studies have examined the relationship between interpersonal political discussion and political knowledge, generally finding that discussion can increase people’s level of political knowledge (Amsalem & Nir, 2019). However, two important questions remain unanswered: (a) Do some types of political discussions facilitate greater levels of political knowledge than others? (b) Do people retain knowledge gained from political discussions? In this study (N = 96), we addressed these questions using a novel methodological approach that combines a lab experiment, in which we manipulated the occurrence of political discussion, with a systematic content analysis of participants’ discussions (N = 1,080 distinct instances of discussions). We found that discussions involving confirmatory feedback and cueing were associated with greater levels of political knowledge than other types of discussions. Furthermore, knowledge gains from discussion were not retained after a short delay. Our study lays the theoretical and methodological groundwork for future investigations into the “black box” of political discussion.
Here for the Right Reasons: The Selection of Women as Peace Delegates
Authors: Brannon Elizabeth , Best Rebecca
Published: February 2022

Since the passing of the United Nations Security Council Resolution 1325 on women, peace, and security more than two decades ago, there has been a global push to bolster the inclusion of women in these processes. When women are selected into peace delegations for the wrong reasons, they—like men—can hinder or stall progress. Yet, very little work has analyzed which women are included in peace processes, how they are selected, why they are selected, and how their individual experiences influence both their behavior and the outcomes of those processes. We identify four selection criteria used to select participants in negotiations: (1) reliability as assessed through either connections to elites or ideological purity; (2) qualifications such as experience in the armed forces, rebel forces, civil society, or academia; (3) personal appeal or ability to elicit sympathy based on factors such as victimhood, attractiveness, youth, or demographics; and (4) selection by a third party whose strength and size have allowed it to negotiate representation in the process. It is likely that in many cases, multiple motives and selection criteria are at play in the selection of individual women (or men). We consider how gender impacts the implementation of these criteria, drawing on a variety of peace processes, but especially the Havana Peace Talks between the Colombian government and Fuerzas Armadas Revolucionarios Colombianos–Ejército del Pueblo. This framework sets the foundation for the development of three research agendas: the first relating to which women get a seat at the table, the second to how the individual backgrounds of the women selected into the peace process influence outcomes, and the third to issues of intersectionality and representation.
Management of elderly patients with head and neck cancer
Authors: Ishii Ryo , Ohkoshi Akira , Kiyota Naomi , Matsuura Kazuto , Yasuda Koichi , Imamura Yoshinori , Saito Yuki , Homma Akihiro ,   the Japan Clinical Oncology Group (JCOG) Head and Neck Cancer Study Group
Published: February 2022

There are no established guidelines for managing older patients with head and neck cancer. Most clinical trials that define current standard therapy included few elderly patients. On the other hand, there is great variability in patients’ comorbidities, physical functions, cognitive function, familial and financial background and values. The key point appears to be appropriate geriatric assessment, clarifying the patients’ outcomes and a multidisciplinary team approach, including the treatment decision-making policy. Although these processes should be scientific in nature, the evidence for the treatment of elderly head and neck patients is very limited.

This review summarizes the evidence available regarding the management of geriatric assessment, each treatment modality and the multidisciplinary team approach for older patients with head and neck cancers.
The prognostic impact of preoperative mean corpuscular volume in colorectal cancer
Authors: Nakamura Kimihiko , Seishima Ryo , Matsui Shimpei , Shigeta Kohei , Okabayashi Koji , Kitagawa Yuko
Published: February 2022

Objective

The relationship between preoperative mean corpuscular volume and postoperative prognosis has been reported in some cancers recently, but no certain consensus has been reached, especially for colorectal cancer. We evaluated the usefulness of mean corpuscular volume as a prognostic factor in colorectal cancer patients.

Methods

This study included 1003 patients with colorectal cancer who underwent curative surgery in a single institution. The relationship between mean corpuscular volume values and postoperative recurrence was evaluated by fractional polynomial model. Based on the result, patients were divided into groups according to mean corpuscular volume values. Clinicopathological factors and long-term outcomes were compared between the groups.

Results

The risk of postoperative recurrence according to mean corpuscular volume value showed a J-shaped curve, suggesting that both low and high mean corpuscular volume have high risk. Low mean corpuscular volume (≤84 fl) group was oncologically advanced in terms of pathological tumor stage, histological grade and lymphatic invasion with higher inflammation markers. High mean corpuscular volume (>95 fl) group had higher frequency of drinking habit with higher values of aspartate aminotransferase, alanine aminotransferase and γ-glutamyl transpeptidase. Abnormal mean corpuscular volume group including these two groups showed significantly worse disease-free survival than the other (P < 0.001). Multivariate analysis suggested that abnormal mean corpuscular volume was an independent risk factor for postoperative recurrence (hazard ratio, 1.344; 95% confidence interval, 1.005–1.796; P = 0.046). Furthermore, its prognostic impact was more significant in pStage III than in other stages.

Conclusion

Preoperative low and high mean corpuscular volume is a poor prognostic factor in colorectal cancer patients. It could be a predictive marker to estimate worse survival outcome after surgery.
Association of Personal Characteristics and Effectiveness of Immunotherapy in Late-Stage Non-Small Cell Lung Cancer: A Systematic Review
Authors: Patel Krishna , Alpert Naomi , Tuminello Stephanie , Taioli Emanuela
Published: February 2022

Background

Although immunotherapy can increase survival in non-small cell lung cancer (NSCLC), response rates are low. It is unclear which characteristics contribute to variability in immunotherapy efficacy and survival. Research is needed to identify reasons for heterogeneity in response rates to better tailor treatments.

Methods

Web of Science, Ovid EMBASE, and MEDLINE were queried from 2013 to January 2021, and all studies reporting overall or progression-free survival for patients treated with immunotherapy for NSCLC of at least stage IIIB were screened.

Results

Included were 18 randomized controlled trials (RCTs; 6534 immunotherapy RCTs; 11 192 nonimmunotherapy RCTs) and 16 observational studies (n = 9073 immunotherapy patients). Among RCTs, there was improved survival with the addition of immunotherapy in patients aged younger than 65 years in 10 of 17 studies; smokers in 8 of 15 studies; and males in 10 of 17 studies and 6 of 17 females. Only 5 studies reported outcomes by race. Among observational studies, younger patients (aged younger than 60, younger than 65, or younger than 70 years in most studies) had better survival than older patients (aged 60 years and older, 65 years and older, or 70 years and older) in 4 of 13 studies, ever-smokers in 7 of 13, and females in 2 of 14. Three studies reported race with mixed results.

Conclusion

Although evidence is mixed, younger patients, smokers, and males may derive more benefit from immunotherapy. Evidence on racial differences is limited. Physicians should be mindful of personal characteristics when formulating treatment plans. Further research is needed to understand underlying mechanisms and to identify the best immunotherapy candidates and alternative treatments for those unlikely to benefit.
Patterns of Objectively Measured Sedentary Time and Emotional Disorder Symptoms Among Youth
Authors: Zink Jennifer , Yang Chih-Hsiang , McAlister Kelsey L , Huh Jimi , Pentz Mary Ann , Page Kathleen A , Belcher Britni R , Dunton Genevieve F
Published: February 2022

Objective

We examined the within-person longitudinal and bidirectional associations between patterns of sedentary time accumulation [alpha (sedentary bout duration/length), sedentary breaks (number of breaks in sedentary bouts)], and symptoms of major depressive disorder and generalized anxiety disorder.

Methods

Children [N = 167, 10.1(0.9) years old, 54.5% female, 59.3% Hispanic, 35.9% overweight/obese at baseline] participated in a 3-year longitudinal study that consisted of assessments of sedentary time, and depressive and anxiety symptoms. At each assessment, participants wore accelerometers and completed the Revised Child Anxiety and Depression Scale. Separate random intercept cross-lagged panel models estimated the within-person longitudinal and bidirectional associations between alpha, sedentary breaks, and symptoms of major depressive disorder and generalized anxiety disorder across chronological age intervals.

Results

Having greater than one’s own usual depressive symptoms at age 11 was associated with fewer sedentary breaks 1 year later. Having greater than one’s own usual generalized anxiety symptoms at age 11 was associated with longer sedentary bouts and fewer sedentary breaks 1 year later. In contrast, having greater than one’s own usual sedentary breaks at age 10 was associated with fewer generalized anxiety symptoms 1 year later. All other associations, including at younger ages, were null.

Conclusion

Deviations from one’s usual levels of depressive or anxiety symptoms may be important for how sedentary time is subsequently accumulated. Intraindividual processes appear to be at play, therefore more within-person research is needed. Intervention strategies for promoting a healthier accumulation of sedentary time may consider targeting occasions when depressive and anxiety symptoms are greater than usual.
Phytochemical profile and protective effects on myocardial ischaemia-reperfusion injury of sweated and non-sweated Salvia miltiorrhiza. Bge alcoholic extracts
Authors: Shan Xiaoxiao , Xiao Yaoyao , Hong Bangzhen , Li Ling , Chen Yueting , Wang Guokai , Yu Nianjun , Peng Daiyin , Zhang Caiyun , Wang Lei , Chen Weidong
Published: February 2022

Objectives

This study aims to compare the fingerprint and the content of the three components of sweated and non-sweated Salvia miltiorrhiza alcoholic extracts (SSAE and NSAE). It also aims to investigate the difference in protective effects of SSAE and NSAE on myocardial ischaemia-reperfusion injury (MIRI).

Methods

The fingerprints of SSAE and NSAE were established by HPLC with a UV detector to identify the common peaks and detect the content of the three major components (cryptotanshinone, tanshinone I and tanshinone IIA). The protective effects of SSAE and NSAE were compared with MIRI rat model after orally administered SSAE and NSAE (2 g/kg of raw drug) for 7 days. The ST segment, PR and QT interval changes and the infarct size were assessed in the rat hearts. Moreover, the activity of aspartate transaminase (AST), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and the level of cardiac troponin I (cTn I) in serum as well as the cardiac H&E staining were evaluated.

Key findings

The results showed that the fingerprints of SSAE and NSAE were similar, and cluster analysis showed that the sweating methods had effects on the alcoholic extracts. The content determination showed that sweating could increase the total content of cryptotanshinone, tanshinone I and tanshinone IIA of S. miltiorrhiza. The results of electrocardiograms (ECG) showed that SSAE could make the ST segment drop more obviously, PR and QT intervals become shorter, and the size of the infarct much smaller. Compared with NSAE, SSAE had more significant effects on the enzymatic activity of AST, LDH and the level of cTn I in serum. The H&E staining showed that both SSAE and NSAE could reduce the degree of heart damage.

Conclusions

The present investigation results demonstrated that sweating increased the content of tanshinone components in S. miltiorrhiza alcoholic extracts, and SSAE had a better protective effect on MIRI.
Swinburne’s Boyishness
Authors: Selleri Andrea
Published: February 2022

This article reconsiders the early critical reception of Algernon Charles Swinburne’s 1866 collection Poems and Ballads with a view to articulating the extent to which the critical hostility that famously greeted the book upon publication was mediated by the category of ‘boyishness’. I show that the complaint that the 29-year-old Swinburne wrote, and by implication thought and felt, too much like a boy and not enough like an adult man lay at the core of the critical onslaught and contributed to underpin critics’ various complaints of obscenity, blasphemy, bad taste and so on. After considering the nature of the connection between the boyish quality often associated with Swinburne as a person throughout his life and the poetical ‘boyishness’ critics perceived in his work, I propose a taxonomy of three main meanings of boyishness that emerge from the early critics’ attacks: boyishness as lack of virility, boyishness as lack of self-restraint, and boyishness as lack of intellectual maturity. By analysing these critical readings in the context of various medical, pedagogical and more broadly cultural discourses of the time, I make the case that Swinburne found himself cast as someone who presented precisely the characteristics of boyhood of which a functioning adult man was supposed to rid himself. The broader argument is that by giving close attention to age-based slurs, we can gain a more fine-grained account of mid-Victorian attitudes to childhood and maturity, and society’s self-image more generally.
Running late: testing delayed supermassive black hole growth models against the quasar luminosity function
Authors: Tillman Megan Taylor , Wellons Sarah , Faucher-Giguère Claude-André , Kelley Luke Zoltan , Anglés-Alcázar Daniel
Published: February 2022

Observations of massive galaxies at low redshift have revealed approximately linear scaling relations between the mass of a supermassive black hole (SMBH) and properties of its host galaxy. How these scaling relations evolve with redshift and whether they extend to lower-mass galaxies, however, remain open questions. Recent galaxy formation simulations predict a delayed, or ‘two-phase,’ growth of SMBHs: slow, highly intermittent BH growth due to repeated gas ejection by stellar feedback in low-mass galaxies, followed by more sustained gas accretion that eventually brings BHs on to the local scaling relations. The predicted two-phase growth implies a steep increase, or ‘kink,’ in BH-galaxy scaling relations at a stellar mass $\rm {M}_{*}\sim 5\times 10^{10}$ M⊙. We develop a parametric, semi-analytic model to compare different SMBH growth models against observations of the quasar luminosity function (QLF) at z ∼ 0.5−4. We compare models in which the relation between SMBH mass and galaxy mass is purely linear versus two-phase models. The models are anchored to the observed galaxy stellar mass function, and the BH mass functions at different redshifts are consistently connected by the accretion rates contributing to the QLF. The best fits suggest that two-phase evolution is significantly preferred by the QLF data over a purely linear scaling relation. Moreover, when the model parameters are left free, the two-phase model fits imply a transition mass consistent with that predicted by simulations. Our analysis motivates further observational tests, including measurements of BH masses and active galactic nuclei activity at the low-mass end, which could more directly test two-phase SMBH growth.
HnRNP D activates production of HPV16 E1 and E6 mRNAs by promoting intron retention
Authors: Cui Xiaoxu , Hao Chengyu , Gong Lijing , Kajitani Naoko , Schwartz Stefan
Published: February 2022

Human papillomavirus type 16 (HPV16) E1 and E6 proteins are produced from mRNAs with retained introns, but it has been unclear how these mRNAs are generated. Here, we report that hnRNP D act as a splicing inhibitor of HPV16 E1/E2- and E6/E7-mRNAs thereby generating intron-containing E1- and E6-mRNAs, respectively. N- and C-termini of hnRNP D contributed to HPV16 mRNA splicing control differently. HnRNP D interacted with the components of splicing machinery and with HPV16 RNA to exert its inhibitory function. As a result, the cytoplasmic levels of intron-retained HPV16 mRNAs were increased in the presence of hnRNP D. Association of hnRNP D with HPV16 mRNAs in the cytoplasm was observed, and this may correlate with unexpected inhibition of HPV16 E1- and E6-mRNA translation. Notably, hnRNP D40 interacted with HPV16 mRNAs in an HPV16-driven tonsillar cancer cell line and in HPV16-immortalized human keratinocytes. Furthermore, knockdown of hnRNP D in HPV16-driven cervical cancer cells enhanced production of the HPV16 E7 oncoprotein. Our results suggest that hnRNP D plays significant roles in the regulation of HPV gene expression and HPV-associated cancer development.