bluuebunny/crossref_metadata_2025 huggingface dataset:
Financing the Transition Toward Carbon Neutrality—an Agent-Based Approach to Modeling Investment Decisions in the Electricity System
Authors: Yang Jinxi , Azar Christian , Lindgren Kristian
Published: December 2021

Transitioning to a low-carbon electricity system requires investments on a very large scale. These investments require access to capital, but that access can be challenging to obtain. Most energy system models do not (explicitly) model investment financing and thereby fail to take this challenge into account. In this study, we develop an agent-based model, where we explicitly include power sector investment financing. We find that different levels of financing constraints and capital availabilities noticeably impact companies' investment choices and economic performances and that this, in turn, impacts the development of the electricity capacity mix and the pace at which CO2emissions are reduced. Limited access to capital can delay investments in low-carbon technologies. However, if the financing constraint is too relaxed, the risk of going bankrupt can increase. In general, companies that anticipate carbon prices too high above or too far below the actual development, along with those that use a low hurdle rate, are the ones that are more likely to go bankrupt. Emissions are cut more rapidly when the carbon tax grows faster, but there is overall a greater tendency for agents to go bankrupt when the tax grows faster. Our energy transition model may be particularly useful in the context of the least financially developed markets.
Ketone Body Metabolism in the Ischemic Heart
Authors: Kolwicz Stephen C.
Published: December 2021

Ketone bodies have been identified as an important, alternative fuel source in heart failure. In addition, the use of ketone bodies as a fuel source has been suggested to be a potential ergogenic aid for endurance exercise performance. These findings have certainly renewed interest in the use of ketogenic diets and exogenous supplementation in an effort to improve overall health and disease. However, given the prevalence of ischemic heart disease and myocardial infarctions, these strategies may not be ideal for individuals with coronary artery disease. Although research studies have clearly defined changes in fatty acid and glucose metabolism during ischemia and reperfusion, the role of ketone body metabolism in the ischemic and reperfused myocardium is less clear. This review will provide an overview of ketone body metabolism, including the induction of ketosis via physiological or nutritional strategies. In addition, the contribution of ketone body metabolism in healthy and diseased states, with a particular emphasis on ischemia-reperfusion (I-R) injury will be discussed.
Characteristics of Aerosol Size Distributions and New Particle Formation Events at Delhi: An Urban Location in the Indo-Gangetic Plains
Authors: Jose Sandhya , Mishra Amit Kumar , Lodhi Neelesh K. , Sharma Sudhir Kumar , Singh Sachchidanand
Published: December 2021

Accurate information about aerosol particle size distribution and its variation under different meteorological conditions are essential for reducing uncertainties related to aerosol-cloud-climate interaction processes. New particle formation (NPF) and the coagulation significantly affect the aerosol size distribution. Here we study the monthly and seasonal variability of aerosol particle size distribution at Delhi from December 2011 to January 2013. Analysis of aerosol particle size distribution using WRAS-GRIMM reveals that aerosol particle number concentration is highest during the post monsoon season owing to the effect of transported crop residue and biomass burning aerosols. Diurnal variations in number concentration show a bimodal pattern with two Aitken mode peaks in all the seasons. Monthly volume size distribution also shows bi-modal distribution with distinct coarse and fine modes. NPF events are observed less frequently in Delhi. Out of 222 days of WRAS data, only 17 NPF events have been observed, with higher NPF frequency during summer season. Growth rate of the nucleation mode of NPF events vary in the range 1.88–21.66 nm/h with a mean value of ∼8.45 ± 5.73 nm/h. It is found that during NPF events the Aitken and nucleation mode particles contribute more to the number concentration. Simultaneous measurement of UV flux and particulate matter (PM10and PM2.5) have also been done along with particle number size distribution measurement to understand the possible mechanisms for NPF events over the study location.
Establishing a Luminescence-Based Chronostratigraphy for the Last Glacial-Interglacial Cycle of the Loess-Palaeosol Sequence Achajur (Armenia)
Authors: Lomax Johanna , Wolf Daniel , Wolpert Urs Tilmann , Sahakyan Lilit , Hovakimyan Hayk , Faust Dominik , Fuchs Markus
Published: December 2021

The loess-palaeosol section Achajur is part of the Sevkar loess area in north-eastern Armenia and comprises three prominent pedocomplexes with intercalated loess units, probably spanning at least three glacial-interglacial cycles. With its central position between the Black Sea and the Caspian Sea, the Sevkar loess area serves as important bridge between loess sections in southeastern Europe and Central Asia. Establishing a numerical chronology of the Achajur loess section enables correlation with other loess-palaeosol sections and a palaeoenvironmental reconstruction of the region. In this paper, we focus on the chronology of the last glacial-interglacial cycle using luminescence dating on fine grain (4–11 µm) material. We compare two post-IR-IRSL protocols on polymineral fine grain using different stimulation temperatures, in order to evaluate the influence of fading and potential unbleachable residuals on our samples. For a backup of the younger ages (<50 ka), we also applied a single aliquot protocol on the quartz fine grain fraction. The results indicate that the upper loess unit was deposited during MIS3 and 4, while MIS2 ages are not present. The underlying pedocomplex and loess unit fall into MIS5 and MIS6, respectively. This confirms that the loess was deposited during dry phases and pedocomplexes formed during wetter phases, as is also shown by comparison with other palaeoenvironmental proxies from the area.
Orientations of Mistaken Point Fronds Indicate Morphology Impacted Ability to Survive Turbulence
Authors: Vixseboxse Philip B. , Kenchington Charlotte G. , Dunn Frances S. , Mitchell Emily G.
Published: December 2021

The Ediacaran fossils of the Mistaken Point E surface have provided crucial insight into early animal communities, including how they reproduced, the importance of Ediacaran height and what the most important factors were to their community dynamics. Here, we use this iconic community to investigate how morphological variation between eight taxa affected their ability to withstand different flow conditions. For each of Beothukis, Bradgatia, Charniodiscus procerus, Charniodiscus spinosus, Plumeropriscum, Primocandelabrum, Thectardis and Fractofusus we measured the orientation and length of their stems (if present) and their fronds. We statistically tested each taxon’s stem and frond orientation distributions to see whether they displayed a uniform or multimodal distribution. Where multimodal distributions were identified, the stem/frond length of each cohort was tested to identify if there were differences in size between different orientation groups. We find that Bradgatia and Thectardis show a bimodal felling direction, and infer that they were felled by the turbulent head of the felling flow. In contrast, the frondose rangeomorphs including Beothukis, Plumeropriscum, Primocandelabrum, and the arboreomorphs were felled in a single direction, indicating that they were upright in the water column, and were likely felled by the laminar tail of the felling flow. These differences in directionality suggests that an elongate habit, and particularly possession of a stem, lent greater resilience to frondose taxa against turbulent flows, suggesting that such taxa would have had improved survivability in conditions with higher background turbulence than taxa like Bradgatia and Thectardis, that lacked a stem and had a higher centre of mass, which may have fared better in quieter water conditions.
Fast High-Resolution S-PTHA Along the Western Mediterranean Sea Coastlines. Application to the Bay of Cannes
Authors: Souty Viviane , Gailler Audrey
Published: December 2021

Probabilistic Tsunami Hazard Assessment (PTHA) is a fundamental framework for producing time-independent forecasts of tsunami hazards at the coast, taking into account local to distant tsunamigenic earthquake sources. If high resolution bathymetry and topography data at the shoreline are available, local tsunami inundation models can be computed to identify the highest risk areas and derive evidence-based evacuation plans to improve community safety. We propose a fast high-resolution Seismic-PTHA approach to estimate the tsunami hazard at a coastal level using the Bay of Cannes as test site. The S-PTHA process is firstly fastened by performing seismic and tsunami hazards separately to allow for quick updates, either from seismic rates by adding new earthquakes, or from tsunami hazard by adding new scenarios of tsunamis. Furthermore, significant tsunamis are selected on the basis of the extrapolation of a tsunami amplitude collected offshore from low-resolution simulations to an a priori amplitude nearshore using Green’s law. This allows a saving in computation time on high-resolution simulations of almost 85%. The S-PTHA performed in the Bay of Cannes exhibits maximum expected tsunami waves that do not exceed 1 m in a 2500-year period, except in some particular places such as the Old Port of Cannes. However, the probability to experience wave heights of 30 cm in this same period exceeds 50% along the main beach of Cannes and these results need to be considered in risk mitigation plans given the high touristic attraction of the area, especially in summer times.
Origin and Characteristics of the Shwetagun Deposit, Modi Taung-Nankwe Gold District and the Kunzeik and Zibyaung Deposits, Kyaikhto Gold District in Mergui Belt, Myanmar: Implications for Fluid Source and Orogenic Gold Mineralization
Authors: Hlaing Myo Kyaw , Yonezu Kotaro , Zaw Khin , Myint Aung Zaw , Aye May Thwe , Watanabe Koichiro
Published: December 2021

The Mergui Belt of Myanmar is endowed with several important orogenic gold deposits, which have economic significance and exploration potential. The present research is focused on two gold districts, Modi Taung-Nankwe and Kyaikhto in the Mergui Belt comparing their geological setting, ore and alteration mineralogy, fluid inclusion characteristics, and ore-forming processes. Both of the gold districts show similarities in nature and characteristics of gold-bearing quartz veins occurring as sheeted veins, massive veins, stockworks to spider veinlets. These gold deposits are mainly hosted by the mudstone, slaty mudstone, greywacke sandstone, slate, and slaty phyllite of Mergui Group (dominantly of Carboniferous age). The gold-bearing quartz veins generally trend from NNE to N-S, whereas some veins strike NW-SE in all deposits. The gold-bearing quartz veins are mainly occurred within the faults and shear zones throughout the two gold districts. Wall-rock alterations at Shwetagun are mainly silicification, chloritization, and sericitization, whereas in Kyaikhto, silicification, carbonation, as well as chloritization, and sericitization are common. At Shwetagun, the gold occurred as electrum grains in fractures within the veins and sulfides. In Kyaikhto, the quartz-carbonate-sulfide and quartz-sulfide veins appeared to have formed from multiple episodes of gold formation categorizing mainly as free native gold grains in fractures within the veins or invisible native gold and electrum within sulfides. At Shwetagun, the ore minerals in the auriferous quartz veins include pyrite, galena, and sphalerite, with a lesser amount of electrum, chalcopyrite, arsenopyrite, chlorite, and sericite. In Kyaikhto, the common mineralogy associated with gold mineralization is pyrite, chalcopyrite, sphalerite, galena, pyrrhotite, arsenopyrite, marcasite, magnetite, hematite, ankerite, calcite, chlorite, epidote, albite, and sericite. At Shwetagun, the mineralization occurred at a varying temperature from 250 to 335°C, with a salinity range from 0.2 to 4.6 wt% NaCl equivalent. The Kyaikhto gold district was formed from aqueous–carbonic ore fluids of temperatures between 242 and 376°C, low to medium salinity (<11.8 wt% NaCl equivalent), and low CO2 content. The ore-forming processes of the Shwetagun deposit in the Modi Taung-Nankwe gold district and the Kyaikhto gold district are remarkably comparable to those of the mesozonal orogenic gold systems.
Hydrocarbon Potential of the Late Permian and the Late Triassic Source Rocks from the Qamdo (Changdu) Basin, Eastern Tibet and Its Linkage with the Sea Level Change
Authors: Qi Zhaolin , Li Yalin , Wang Chengshan
Published: December 2021

The Qamdo Basin in eastern Tibet has significant petroleum potential and previous studies indicate that the basin contains thick potential source rocks of the Late Permian and the Late Triassic ages. In this paper, the petroleum potential of samples from measured the Upper Permian and Upper Triassic outcrop sections were evaluated on the basis of sedimentological, organic petrographic and geochemical analyses. Initial evaluations of total organic carbon contents indicated that shale samples from the Upper Permian Tuoba Formation and the Upper Triassic Adula and Duogala Formations have major source rock potential, while carbonate rocks from the Upper Triassic Bolila Formation are comparatively lean in organic matter More detailed analyses of OM-rich shale samples from the Tuoba, Adula and Duogala Formations included Rock-eval, elemental analyses, gas chromatography and organic petrography. Maceral compositions and plots of atomic O/C versus H/C indicate that the organic matter present in the samples is primarily Type II with a mixed source. Analyses of acyclic isoprenoid biomarkers indicate the organic matter was deposited under reducing and sub-to anoxic conditions. Based on the high vitrinite reflectance (Ro>1.3%) and Rock-eval data, the samples are classified as highly to over-mature, suggesting that the Tuoba, Adula and Duogaila Formation shales may generate thermogenic gas. Source rock intervals in the three formations are interpreted to have been deposited in marginal-marine environment during transgressions and under a warm and moist climatic condition.
Motivating Self-Efficacy in Diverse Biomedical Science Post-baccalaureate and Graduate Students Through Scientific Conference Implementation
Authors: Boehmer Kasey R. , Boschen De Souza Suelen Lucio , Doles Jason D. , Lachman Nirusha , Mays Dennis , Hedin Karen E. , Dornink Cheryl A. , Maher Louis J. , Lujan J. Luis
Published: December 2021

Tactics to increase the number of underrepresented (UR) students in biomedical research PhD training programs have not yet translated to UR faculty numbers that reflect the diversity of the United States. Continued interventions are required to build skills beyond those that result in placement into a PhD program. We hypothesize that successful interventions must build skills that give UR students foundations for confident self-efficacy in leadership. We seek interventions that allow UR students to envision themselves as successful faculty. We posit that development of such skills is difficult in the classroom or laboratory alone. Therefore, novel interventions are required. As part of the NIH-funded Post-baccalaureate Research Education Program (PREP) and Initiative for Maximizing Student Development (IMSD) at the Mayo Clinic Graduate School of Biomedical Sciences, we designed and implemented a unique intervention to support development of student leadership skills: a biannual student-organized and student-led national research conference titled “Scientific Innovation Through Diverse Perspectives” (SITDP). This initiative is based on the concept that students who actively live out realistic roles as scientific leaders will be encouraged to persist to scientific leadership as faculty. Here we describe the motivation for, design of, and outcomes from, the first three pilot conferences of this series. We further discuss approaches needed to rigorously evaluate the effectiveness of such interventions in the future.
Social Well-Being at School: Development and Validation of a Scale for Primary Education Students
Authors: Moliner Lidon , Alegre Francisco , Cabedo-Mas Alberto , Chiva-Bartoll Oscar
Published: December 2021

This study presents the development and validation of a scale for Primary Education students that measures social well-being. A seven-factor structure was defined, with the factors being: achievement, cooperation, cohesion, coexistence, attitude towards school, attitude towards diversity and solidarity. 14 experts from independent European universities participated in the validation process of the scale. The 38-item scale showed considerable reliability (Cronbach’s alpha =0.91). The confirmatory factor analysis confirmed the original seven-factor structure with consistent goodness and badness of fit indexes. The promising results in this study suggest that this scale may be suitable for an international audience.
Red Blood Cell Count: An Unrecognized Risk Factor for Nonalcoholic Fatty Liver Disease
Authors: Zhong Fang , Guan Liying , Lin Haiyan , Zhao Meng , Qin Yiming , Li Qihang , Yuan Zhongshang , Yang Guang , Gao Ling , Zhao Jiajun
Published: December 2021

Objective

Nonalcoholic fatty liver disease (NAFLD) is becoming a global public health challenge. A convenient NAFLD indicator will greatly facilitate risk appraisal and prevention. As a readily available and inexpensive hematological index in routine clinical examinations, red blood cells (RBCs) are gaining increasing attention in many diseases, such as metabolic syndrome, but their association with NAFLD is unknown.

Methods

This health management cohort study included 27,112 subjects (17,383 non-NAFLD and 9,729 NAFLD) with up to 5 years of follow-up (median 2.8 years). NAFLD was diagnosed by ultrasonography. NAFLD severity was categorized as mild, moderate, or severe. The generalized estimation equation (GEE), an extension of generalized linear models that allows for analysis of repeated measurements, was used to analyze the association between RBC count and NAFLD.

Results

Overall, 4,332 of 17,383 (24.9%) subjects without NAFLD at baseline developed NAFLD. Incident NAFLD risk was positively associated with RBC count. After adjustment for hemoglobin and other confounders, the risk of incident NAFLD was 21%, 32%, and 51% higher in the second, third, and fourth RBC count quartiles, respectively, than in the lowest quartile. In 1,798 of 9,476 (19.0%) subjects with NAFLD at baseline, the severity of NAFLD increased. NAFLD progression risk increased progressively as RBC count increased (P for trend < 0.001). Every one-unit (1012 cells/L) increase in RBC count was associated with a 53% [OR 1.53 (95% CI 1.32-1.77)] increased risk for NAFLD progression.

Conclusions

Elevated RBC count was independently associated with a high risk of NAFLD incidence and progression. This finding revealed a convenient NAFLD risk indicator.
Discovery of a Positive Allosteric Modulator of Cholecystokinin Action at CCK1R in Normal and Elevated Cholesterol
Authors: Harikumar Kaleeckal G. , Coudrat Thomas , Desai Aditya J. , Dong Maoqing , Dengler Daniela G. , Furness Sebastian G. B. , Christopoulos Arthur , Wootten Denise , Sergienko Eduard A. , Sexton Patrick M. , Miller Laurence J.
Published: December 2021

Drugs useful in prevention/treatment of obesity could improve health. Cholecystokinin (CCK) is a key regulator of appetite, working through the type 1 CCK receptor (CCK1R); however, full agonists have not stimulated more weight loss than dieting. We proposed an alternate strategy to target this receptor, while reducing likelihood of side effects and/or toxicity. Positive allosteric modulators (PAMs) with minimal intrinsic agonist activity would enhance CCK action, while maintaining spatial and temporal characteristics of physiologic signaling. This could correct abnormal stimulus–activity coupling observed in a high-cholesterol environment observed in obesity. We utilized high-throughput screening to identify a molecule with this pharmacological profile and studied its basis of action. Compound 1 was a weak partial agonist, with PAM activity to enhance CCK action at CCK1R, but not CCK2R, maintained in both normal and high cholesterol. Compound 1 (10 µM) did not exhibit agonist activity or stimulate internalization of CCK1R. It enhanced CCK activity by slowing the off-rate of bound hormone, increasing its binding affinity. Computational docking of Compound 1 to CCK1R yielded plausible poses. A radioiodinatable photolabile analogue retained Compound 1 pharmacology and covalently labeled CCK1R Thr211, consistent with one proposed pose. Our study identifies a novel, selective, CCK1R PAM that binds to the receptor to enhance action of CCK-8 and CCK-58 in both normal and disease-mimicking high-cholesterol environments. This facilitates the development of compounds that target the physiologic spatial and temporal engagement of CCK1R by CCK that underpins its critical role in metabolic regulation.
Informed Consent for Intraoperative Neural Monitoring in Thyroid and Parathyroid Surgery – Consensus Statement of the International Neural Monitoring Study Group
Authors: Wu Che-Wei , Huang Tzu-Yen , Randolph Gregory W. , Barczyński Marcin , Schneider Rick , Chiang Feng-Yu , Silver Karcioglu Amanda , Wojtczak Beata , Frattini Francesco , Gualniera Patrizia , Sun Hui , Weber Frank , Angelos Peter , Dralle Henning , Dionigi Gianlorenzo
Published: December 2021

In the past decade, the use of intraoperative neural monitoring (IONM) in thyroid and parathyroid surgery has been widely accepted by surgeons as a useful technology for improving laryngeal nerve identification and voice outcomes, facilitating neurophysiological research, educating and training surgeons, and reducing surgical complications and malpractice litigation. Informing patients about IONM is not only good practice and helpful in promoting the efficient use of IONM resources but is indispensable for effective shared decision making between the patient and surgeon. The International Neural Monitoring Study Group (INMSG) feels complete discussion of IONM in the preoperative planning and patient consent process is important in all patients undergoing thyroid and parathyroid surgery. The purpose of this publication is to evaluate the impact of IONM on the informed consent process before thyroid and parathyroid surgery and to review the current INMSG consensus on evidence-based consent. The objective of this consensus statement, which outlines general and specific considerations as well as recommended criteria for informed consent for the use of IONM, is to assist surgeons and patients in the processes of informed consent and shared decision making before thyroid and parathyroid surgery.
Economic Dispatch Methods for Smart Grid Based on Improved SPEA2 and Improved NSGA2
Authors: Li Bo , Wang Jingwen
Published: December 2021

The severity of the ongoing environmental crisis has prompted the development of renewable energy generation and smart grids integration. The access of enewable energy makes the economic dispatching of smart grid complicated. Therefore, the economic dispatching model for smart grid is very necessary. This paper presents an economic dispatching model of smart power grid, which considers both economy and pollution emission. The smart grid model used for the simulation is construced of wind energy, solar energy, fuel cell, and thermal power, and the use of fuel cell enables the smart grid to achieve multi-energy complementar. To overcome the defect of the traditional centralized communication methods, which are prone to communication jams, this paper adopts a multi-agent inform ation exchange method to improve the stability and efficiency. In terms of the solution method for this model, this paper proposes Improved Strength Pareto Evolutionary Algorithm 2(ISPEA2) and Improved Non-dominated Sorting Genetic Algorithm 2(INSGA2) that solves the economic dispatch problem of a smart grid. The strength Pareto evolutionary algorithm 2(SPEA2),non-dominated sorting genetic algorithm 2(NSGA2) and the improved algorithms are simultaneously applied to the proposed smart grid model for economic dispatching simulation. The simulation results show that ISPEA2 and INSGA2 are effective. ISPEA2 and INSGA2 have shown improvements over SPEA2 and NSGA2 in accuracy or running times.
A Fusion-Based Method of State-of-Charge Online Estimation for Lithium-Ion Batteries Under Low Capacity Conditions
Authors: Zhou Nan , Liang Hong , Cui Jing , Chen Zeyu , Fang Zhiyuan
Published: December 2021

The accurate estimation of the battery state of charge (SOC) is crucial for providing information on the performance and remaining range of electric vehicles. Based on the analysis of battery charge and discharge data under actual vehicle driving cycles, this paper presents an online estimation method of battery SOC based on the extended Kalman filter (EKF) and neural network (NN). A battery model is established to identify and calibrate battery parameters. SOC estimation is conducted in the low-SOC area by exploring the relationship between battery parameters and SOC through many experimental results. In the fusion online estimation method, the NN is carried out to propose the estimation as the global mainstream trend providing a high precision feasible region; the EKF algorithm is used to provide the initial assessment and the local fluctuation boundary revision. Verified results show that it can improve the SOC estimation in low-battery capacity accuracy. It has achieved good adaptability to the estimation accuracy of low battery capacity SOC in different cycle conditions.
Influence of Late Holocene Climate Change and Human Land Use on Terrestrial and Aquatic Ecosystems in Southwest Madagascar
Authors: Domic Alejandra I. , Hixon Sean W. , Velez Maria I. , Ivory Sarah J. , Douglass Kristina G. , Brenner Mark , Curtis Jason H. , Culleton Brendan J. , Kennett Douglas J.
Published: December 2021

Madagascar’s biota underwent substantial change following human colonization of the island in the Late Holocene. The timing of human arrival and its role in the extinction of megafauna have received considerable attention. However, the impacts of human activities on regional ecosystems remain poorly studied. Here, we focus on reconstructing changes in the composition of terrestrial and aquatic ecosystems to evaluate the impact of human land use and climate variability. We conducted a paleoenvironmental study, using a sediment record that spans the last ∼1,145 years, collected from a lakebed in the Namonte Basin of southwest Madagascar. We examined physical (X-ray fluorescence and stratigraphy) and biotic indicators (pollen, diatoms and micro- and macro-charcoal particles) to infer terrestrial and aquatic ecosystem change. The fossil pollen data indicate that composition of grasslands and dry deciduous forest in the region remained relatively stable during an arid event associated with northward displacement of the Intertropical Convergence Zone (ITCZ) between ∼1,145 and 555 calibrated calendar years before present (cal yr BP). Charcoal particles indicate that widespread fires occurred in the region, resulting from a combination of climate drivers and human agency during the entire span covered by the paleorecord. Following settlement by pastoral communities and the disappearance of endemic megafauna ∼1,000 cal yr BP, grasslands expanded and the abundance of trees that rely on large animals for seed dispersal gradually declined. A reduction in the abundance of pollen taxa characteristic of dry forest coincided with an abrupt increase in charcoal particles between ∼230 and 35 cal yr BP, when agro-pastoral communities immigrated into the region. Deforestation and soil erosion, indicated by a relatively rapid sedimentation rate and high K/Zr and Fe/Zr, intensified between 180 and 70 cal yr BP and caused a consequent increase in lake turbidity, resulting in more rapid turnover of the aquatic diatom community. Land use and ongoing climate change have continued to transform local terrestrial and freshwater ecosystems during the last ∼70 years. The current composition of terrestrial and aquatic ecosystems reflects the legacy of extinction of native biota, invasion of exotic species, and diminished use of traditional land management practices.
Directional Selection on Tree Seedling Traits Driven by Experimental Drought Differs Between Mesic and Dry Populations
Authors: Costa e Silva João , Jordan Rebecca , Potts Brad M. , Pinkard Elizabeth , Prober Suzanne M.
Published: December 2021

We evaluated population differences and drought-induced phenotypic selection on four seedling traits of the Australian forest treeEucalyptus pauciflorausing a glasshouse dry-down experiment. We compared dry and mesic populations and tested for directional selection on lamina length (reflecting leaf size), leaf shape, the node of ontogenetic transition to the petiolate leaf (reflecting the loss of vegetative juvenility), and lignotuber size (reflecting a recovery trait). On average, the dry population had smaller and broader leaves, greater retention of the juvenile leaf state and larger lignotubers than the mesic population, but the populations did not differ in seedling survival. While there was statistical support for directional selection acting on the focal traits in one or other population, and for differences between populations in selection gradient estimates for two traits, only one trait—lamina length—exhibited a pattern of directional selection consistent with the observed population differences being a result of past adaptation to reduce seedling susceptibility to acute drought. The observed directional selection for lamina length in the mesic population suggests that future increases in drought risk in the wild will shift the mean of the mesic population toward that of the dry population. Further, we provide evidence suggesting an early age trade-off between drought damage and recovery traits, with phenotypes which develop larger lignotubers early being more susceptible to drought death. Such trade-offs could have contributed to the absence of population mean differences in survival, despite marked differentiation in seedling traits.
Fluid Nest Membership Drives Variable Relatedness in Groups of a Facultatively Social Bee
Authors: Ostwald Madeleine M. , Dahan Romain A. , Shaffer Zachary , Fewell Jennifer H.
Published: December 2021

Kin selection theory has dominated our understanding of the evolution of group living. However, many animal groups form among non-relatives, which gain no indirect fitness benefits from cooperating with nestmates. In this study, we characterized the relatedness and inter-nest migration behavior of the facultatively social carpenter bee, Xylocopa sonorina. Nesting constraints due to costly nest construction in this species give rise to intense intraspecific competition over access to existing nests. We used mark-recapture techniques to characterize patterns of dispersal and nest relocation within a nesting aggregation of spatially clustered nests. Two-thirds of bees relocated at least once during the reproductive season, likely to seek reproductive opportunities in another nest. This fluid nest membership creates opportunities for association among non-relatives. To assess the effects of this dynamic nesting behavior on group relatedness, we used microsatellite analysis to estimate relative relatedness within and between nests in the aggregation. We found that relatedness was variable across sampling years, but that in many cases nestmates were no more related to one another than they were to non-nestmate bees in the population. Together, these results suggest that group composition in X. sonorina may result from strategies to maximize direct fitness. This study supports the hypothesis that factors beyond kinship, such as ecological constraints, are likely to drive group formation in this species.
Stem Radial Growth Is Negatively Related to Tree Defoliation and Damage in Conifers, Northern Italy
Authors: Ferretti Marco , Ghosh Sucharita , Gottardini Elena
Published: December 2021

Although several manipulative experiments provided evidence for a negative effect of defoliation on tree growth, results from observational studies were less univocal. This may be due to the ability of observed defoliation to reflect the health status of individual trees, to the influence of site condition and to the amplitude of time window used for growth assessment. Here, we investigated the relationship between two tree health indicators (crown defoliation, damage symptoms) and annual (measured by tree-ring width on 69 Norway spruce trees) and periodical (5-year and 10-year diameter increments, 346 trees from five coniferous species) tree growth. Data originated from 14 (seven for tree rings) ICP Forests Level I plots in Trentino, northern Italy. Diameter, defoliation and damage were measured between 1997 and 2011 as part of the annual crown condition survey, while cores for tree-rings were collected on a sub-sample of trees in 2012. We carried out regression modeling combined with model selection in one-step (periodical data) and two-step (annual data) approaches, using moving averages for the annual data with varying time window widths. Our results indicated an overall negative correlation between defoliation and annual or periodical stem diameter growth. The relationship between defoliation and growth changes in relation to the time window considered, and becomes stronger when data are aggregated over longer time windows (>3 years), when also the occurrence of damage symptoms plays a significant role. The effect of the amplitude of the time windows for data aggregation is probably due to the mechanisms behind the defoliation-growth relationship, which may change according to the causal factors involved. In particular, when larger time windows are considered, short-term fluctuations are likely to be smoothed out, and more general patterns may emerge. We concluded that radial growth is significantly negatively related to defoliation, and this supports the use of defoliation as a rapid indicator for forest health and vitality.
Parental Somatic Mosaicism Uncovers Inheritance of an Apparently De Novo GFAP Mutation
Authors: Grossi Alice , Morelli Federico , Di Duca Marco , Caroli Francesco , Moroni Isabella , Tonduti Davide , Bachetti Tiziana , Ceccherini Isabella
Published: December 2021

Alexander disease is a leukodystrophy caused by heterozygous mutations of GFAP gene. Recurrence in siblings from healthy parents provides a confirmation to the transmission of variants through germinal mosaicism. With the use of DNA isolated from peripheral blood, next-generation sequencing (NGS) of GFAP locus was performed with deep coverage (≥500×) in 11 probands and their parents (trios) with probands heterozygous for apparently de novo GFAP mutations. Indeed, one parent had somatic mosaicism, estimated in the range of 8.9%–16%, for the mutant allele transmitted to the affected sibling. Parental germline mosaicism deserves attention, as it is critical in assessing the risk of recurrence in families with Alexander disease.
A Highly Conserved Peptide Vaccine Candidate Activates Both Humoral and Cellular Immunity Against SARS-CoV-2 Variant Strains
Authors: Gao Fengxia , Huang Jingjing , Li Tingting , Hu Chao , Shen Meiying , Mu Song , Luo Feiyang , Song Shuyi , Hao Yanan , Wang Wang , Han Xiaojian , Qian Chen , Wang Yingming , Wu Ruixin , Li Luo , Li Shenglong , Jin Aishun
Published: December 2021

Facing the imminent need for vaccine candidates with cross-protection against globally circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutants, we present a conserved antigenic peptide RBD9.1 with both T-cell and B-cell epitopes. RBD9.1 can be recognized by coronavirus disease 2019 (COVID-19) convalescent serum, particularly for those with high neutralizing potency. Immunization with RBD9.1 can successfully induce the production of the receptor-binding domain (RBD)-specific antibodies in Balb/c mice. Importantly, the immunized sera exhibit sustained neutralizing efficacy against multiple dominant SARS-CoV-2 variant strains, including B.1.617.2 that carries a point mutation (SL452R) within the sequence of RBD9.1. Specifically, SY451 and SY454 are identified as the key amino acids for the binding of the induced RBD-specific antibodies to RBD9.1. Furthermore, we have confirmed that the RBD9.1 antigenic peptide can induce a S448-456 (NYNYLYRLF)-specific CD8+ T-cell response. Both RBD9.1-specific B cells and the S448-456-specific T cells can still be activated more than 3 months post the last immunization. This study provides a potential vaccine candidate that can generate long-term protective efficacy over SARS-CoV-2 variants, with the unique functional mechanism of activating both humoral and cellular immunity.
Coiling-Up Space Metasurface for High-Efficient and Wide-angle Acoustic Wavefront Steering
Authors: Chen Shuang , Fan Yuancheng , Yang Fan , Sun Kangyao , Fu Quanhong , Zheng Jianbang , Zhang Fuli
Published: December 2021

The recent advent of acoustic metasurface displays tremendous potential with their unique and flexible capabilities of wavefront manipulations. In this paper, we propose an acoustic metagrating made of binary coiling-up space structures to coherently control the acoustic wavefront steering. The acoustic wave steering is based on the in-plane coherent modulation of waves in different diffraction channels. The acoustic metagrating structure with a subwavelength thickness is realized with 3D printed two coiling-up space metaunits. By adjusting structural parameters of the metaunits, the −1st-order diffraction mode can be retained, and the rest of the diffraction orders are eliminated as much as possible through destructive interference, forming a high-efficiency anomalous reflection in the scattering field. The anomalous reflection performance of the designed metagrating is achieved over a wide range of incident angles with high efficiency.
Prevalence and Prognostic Significance of Hyponatremia in Patients With Lung Cancer: Systematic Review and Meta-Analysis
Authors: Bartalis Eszter , Gergics Marin , Tinusz Benedek , Földi Mária , Kiss Szabolcs , Németh Dávid , Solymár Margit , Szakács Zsolt , Hegyi Péter , Mezösi Emese , Bajnok László
Published: December 2021

Background: The prevalence of hyponatremia is highly variable among patients with lung cancer. However, its prevalence and prognostic significance in subgroups of patients with lung cancer have not yet been evaluated in a meta-analysis.

Methods: We have registered our meta-analysis and review protocol to the PROSPERO International Prospective Register of Systematic Reviews, with the following registration number: CRD42020167013. A systematic search was done in the following sources: MEDLINE, Embase, CENTRAL, Web of Science, ClinicalTrials.gov, a WHO Global Health Library.

Results: We identified a total of 8,962 potentially eligible studies, and we included 31 articles in our evaluation. The prevalence of hyponatremia in patients with lung cancer varied between 3 and 94.8% with an average of 25% without any significant differences between the following subgroups: histotype, gender, age, Eastern Cooperative Oncology Group (ECOG) state, and the extent of disease. The overall survival (OS) was significantly lower in hyponatremic compared to normonatremic patients at 10 months [RR.59 (95% CI.47–0.74), p < 0.001] and at 20 months [RR.44 (95% CI.33–0.59), p < 0.001], with worse survival rates in non-small cell lung cancer (NSCLC) [RR.27 (95% CI.12–0.44), p < 0.001] than in small cell lung cancer (SCLC) [RR.42 (95% CI.27–0.57), p < 0.001]. If hyponatremia was corrected, OS at 10 months was significantly higher than in the uncorrected hyponatremia group [RR 1.83 (95% CI 1.37–2.44), p < 0.001], but, at 20 months, no statistically significant difference could be found between these subgroups [RR 2.65 (95% CI.94–7.50), p = 0.067].

Conclusions: Patients with lung cancer diagnosed with hyponatremia, especially patients with NSCLC, seem to have significantly lower survival rates than normonatremic patients. If hyponatremia remains uncorrected, the mortality rates might be even higher.
A Novel Approach for COVID-19 Patient Condition Tracking: From Instant Prediction to Regular Monitoring
Authors: Bakin Evgeny A. , Stanevich Oksana V. , Chmelevsky Mikhail P. , Belash Vasily A. , Belash Anastasia A. , Savateeva Galina A. , Bokinova Veronika A. , Arsentieva Natalia A. , Sayenko Ludmila F. , Korobenkov Evgeny A. , Lioznov Dmitry A. , Totolian Areg A. , Polushin Yury S. , Kulikov Alexander N.
Published: December 2021

Purpose: The aim of this research is to develop an accurate and interpretable aggregated score not only for hospitalization outcome prediction (death/discharge) but also for the daily assessment of the COVID-19 patient's condition.

Patients and Methods: In this single-center cohort study, real-world data collected within the first two waves of the COVID-19 pandemic was used (27.04.2020–03.08.2020 and 01.11.2020–19.01.2021, respectively). The first wave data (1,349 cases) was used as a training set for the score development, while the second wave data (1,453 cases) was used as a validation set. No overlapping cases were presented in the study. For all the available patients' features, we tested their association with an outcome. Significant features were taken for further analysis, and their partial sensitivity, specificity, and promptness were estimated. Sensitivity and specificity were further combined into a feature informativeness index. The developed score was derived as a weighted sum of nine features that showed the best trade-off between informativeness and promptness.

Results: Based on the training cohort (median age ± median absolute deviation 58 ± 13.3, females 55.7%), the following resulting score was derived: APTT (4 points), CRP (3 points), D-dimer (4 points), glucose (4 points), hemoglobin (3 points), lymphocytes (3 points), total protein (6 points), urea (5 points), and WBC (4 points). Internal and temporal validation based on the second wave cohort (age 60 ± 14.8, females 51.8%) showed that a sensitivity and a specificity over 90% may be achieved with an expected prediction range of more than 7 days. Moreover, we demonstrated high robustness of the score to the varying peculiarities of the pandemic.

Conclusions: An extensive application of the score during the pandemic showed its potential for optimization of patient management as well as improvement of medical staff attentiveness in a high workload stress. The transparent structure of the score, as well as tractable cutoff bounds, simplified its implementation into clinical practice. High cumulative informativeness of the nine score components suggests that these are the indicators that need to be monitored regularly during the follow-up of a patient with COVID-19.
Creating Clinical Reasoning Assessment Tools in Different Languages: Adaptation of the Pediatric Emergency Medicine Script Concordance Test to Japanese
Authors: Nomura Osamu , Itoh Taichi , Mori Takaaki , Ihara Takateru , Tsuji Satoshi , Inoue Nobuaki , Carrière Benoit
Published: December 2021

Introduction: Clinical reasoning is a crucial skill in the practice of pediatric emergency medicine and a vital element of the various competencies achieved during the clinical training of resident doctors. Pediatric emergency physicians are often required to stabilize patients and make correct diagnoses with limited clinical information, time and resources. The Pediatric Emergency Medicine Script Concordance Test (PEM-SCT) has been developed specifically for assessing physician's reasoning skills in the context of the uncertainties in pediatric emergency practice. In this study, we developed the Japanese version of the PEM-SCT (Jpem-SCT) and confirmed its validity by collecting relevant evidence.

Methods: The Jpem-SCT was developed by translating the PEM-SCT into Japanese using the Translation, Review, Adjudication, Pretest, Documentation team translation model, which follows cross-cultural survey guidelines for proper translation and cross-cultural and linguistic equivalences between the English and Japanese version of the survey. First, 15 experienced pediatricians participated in the pre-test session, serving as a reference panel for modifying the test descriptions, incorporating Japanese context, and establishing the basis for the scoring process. Then, a 1-h test containing 60 questions was administered to 75 trainees from three academic institutions. Following data collection, we calculated the item-total correlations of the scores to optimize selection of the best items in the final version of the Jpem-SCT. The reliability of the finalized Jpem-SCT was calculated using Cronbach's α coefficient for ensuring generalizability of the evidence. We also conducted multiple regression analysis of the test score to collect evidence on validity of the extrapolation.

Results: The final version of the test, based on item-total correlation data analysis, contained 45 questions. The participant's specialties were as follows: Transitional interns 12.0%, pediatric residents 56.0%, emergency medicine residents 25.3%, and PEM fellows 6.7%. The mean score of the final version of the Jpem-SCT was 68.6 (SD 9.8). The reliability of the optimized test (Cronbach's α) was 0.70. Multiple regression analysis showed that being a transitional intern was a negative predictor of test scores, indicating that clinical experience relates to performance on the Jpem-SCT.

Conclusion: This pediatric emergency medicine Script Concordance Test was reliable and valid for assessing the development of clinical reasoning by trainee doctors during residency training.