bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative assessment of veneral disease research laboratory (VDRL) and point of care (POC) tests for diagnosing syphilis among pregnant women at a tertiary health facility in Lagos: A retrospective cohort study
Authors: Harrison Nkechinyere , Ugwu Aloy Okechukwu , Okunade K.S. , Ismail Lawal , Ayanbode Olufemi , Chinenye Elekwa , Igbodike E.P. , Oluwole A.A. , Kusamotu O.A. , Anas R.F. , Ani-Ugwu Nneoma K. , Adebayo Williams Awoniyi , Oluwaseun Alakija , Okeke C.J. , Taiwo Ajani , Ngwu Hilary O. , Chinelo Okoye , Oghenefejiro Aisini Abigail , Gloria Orjiakor Chioma , Ige S. , Ezeoke Chibunna Denzel , Aidevo Ogbekhuemen Martha
Published: January 2024

Background: Syphilis is a systemic infection caused by the Spirochete Treponema Pallidum. The infection is of particular concern during pregnancy because of the high risk of vertical transmission causing adverse outcome including death. Traditional screening method is with Venereal Disease Research Laboratory (VDRL), which has a high false positive result and an increased waiting time. Aim: To compare the syphilis detection rate between Point-of-Care testing and Venereal Disease Research Laboratory testing among pregnant women. Methods: The retrospective cohort study was conducted at 68 Nigerian Army Reference Hospital Yaba (68NARHY), Lagos between July 2021 and June 2023. Data from antenatal clinic records were retrieved. All eligible pregnant women had both POC and VDRL testing. The primary outcome measures were false positive, false negative, true positive, true negative of the two tests. The sensitivity, and specificity and the diagnostic accuracy of both tests were calculated following which the ROC curve was drawn. Women with incomplete record or who did not conduct both testing were excluded from the study. All data set were collected using a well-designed purpose driven proforma. The data set were stored and later analyzed using the IBM SPSS for Windows, version 26.0 Armonk, NY: IBM corp. Analysis was both descriptive and inferential. The p-value of < 0.05 was set as statistically significant levels. Result: The mean age of the participants was 31.30±5.3. About 45.5% were of low parity while military personnel consisted of more than half of the study population. The prevalence of syphilis was 1.8% and 4.3% using VDRL and POC test kits respectively. When compared, there was a significant difference (P<0.001). It was also noted that a greater proportion of military personnel were reactive to the screening using either of those kits as against civilians. Conclusion: In our study, POC has demonstrated its ability to detect more cases of syphilis than VDRL as a screening test. Therefore, POC may serve as a cost-effective replacement screening test for VDRL, to rapidly and accurately detect syphilis infection. This step is crucial for early diagnosis, prompt treatment, and the prevention of further transmission of syphilis.
Solitary lymph-nodal para-aortic recurrence in a patient with High Grade Serous Ovarian Cancer
Authors: Fanaki Maria , Perros Paraskevas , Pergialiotis Vasilios , Mpramis Konstantinos , Thomakos Nikolaos
Published: January 2024

Ovarian cancer is the second most common cause of gynecologic cancer death. The most common histologic category of epithelial ovarian cancer is High Grade Serous Ovarian Cancer (HGSOC). The initial management usually involves appropriate staging and debulking surgery. Otherwise, patients with unresectable disease receive neoadjuvant chemotherapy, and then undergo Interval Debulking Surgery (IDS). However, even cases with complete clinical response often experience a recurrence. While the primary debulking surgery is the standard of treatment, the role of secondary debulking surgery for recurrent ovarian cancer is still debatable. The most common sites of recurrences are pelvis and abdomen, whereas recurrence confined solely to lymph node is a rare event. Secondary cytoreductive surgery (SCS) for recurrent ovarian cancer presenting as isolated lymph node metastases has been associated with favorable long-term survival, although the resection of aortic metastatic lymph nodes could be related to severe intraoperative complications. In this article, we report a case of a 72-year-old patient with an isolated lymph-nodal para-aortic recurrence with a history of primary debulking surgery for high grade serous ovarian cancer FIGO stage IV, four years before, followed by received adjuvant chemotherapy, and maintenance therapy. The patient presented with a lymph node relapse down the ileocolic artery and submitted to radical resection of the metastatic lymph nodes.
Program Fisioterapi Pada Atlet Setelah Infeksi Covid-19 Dengan Cardiac Problem Untuk Dapat Kembali Ke Olahraga : Studi Literatur
Authors: Pahlawi Riza , Sakanaher Alfuadi , Sativani Zahra
Published: January 2024

Covid-19 merupakan penyakit yang disebabkan oleh virus Sars-CoV-2 yang menyerang sistem pernafasan. Virus ini ternyata juga dapat menyebabkan komplikasi seperti renal failure dan cardiac problem. Setelah infeksi Covid-19 seorang atlet sangat bersiko mengalami cardiac problem dikarenakan olahraga intensitas tinggi yang mereka lakukan dapat meningkatkan penyebaran virus menuju jantung, inflamasi, kematian sel, dan aritmia. Oleh karena itu, pentingnya memastikan kondisi jantung atlet dalam kondisi normal sebelum kembali melakukan aktifitas fisik. Studi ini bertujuan untuk menjelaskan bagaimana Program fisioterapi pada Atlet yang memiliki Cardiac problem setelah infeksi Covid-19 untuk dapat kembali berolahraga. Penelitian ini menggunakan metode Literature review dengan strategi pencarian PICO Framework. Dari hasil pencarian didapatkan 1664 jurnal yang kemudian disaring menggunakan kriteria inklusi dan eksklusi sehingga didapatkan 5 jurnal untuk dilakukan sintesis. Hasil dari studi ini meliputi program fisioterapi pada atlet yang mengalami cardiac problem setelah infeksi Covid-19 yaitu program dengan intensitas Low to moderate yang dilakukan selama 3 sampai 6 bulan. Kesimpulan studi ini yaitu Ketika Atlet terinfeksi Covid-19 dan mengalami cardiac problem maka Program fisioterapi yang direkomendasikan berupa latihan dengan intensitas low to moderate untuk membantu atlet tetap mempertahankan kondisi kapasitas fungsionalnya dan memberi waktu pada jantung untuk recovery sehingga atlet dapat kembali berolahraga lagi.
Beda Pengaruh Antara Latihan Open Kinetic Chain Dan Latihan Isometrik Pada Otot Quadriceps Terhadap Penurunan Nyeri Penderita Osteoartritis Knee
Authors: Haryatno Pajar , Maryono Maryono , Putri Nitaya
Published: January 2024

Kerusakan struktur sendi pada kasus osteoartritis lutut akan menstimulasi mekanoreseptor menghantarkan informasi sensoris yang abnormal. Pada akhirnya menyebabkan penurunan kontraksi volunter otot quadriceps femoris yang diikuti oleh penurunan kekuatan otot quadriceps femoris. Penurunan kekuatanotot quadriceps femoris juga mempengaruhi nyeri saat beraktivitas pada penderita osteoartritis lutut. Tujuan: Untuk mengetahui perbedaan pengaruh latihan open kinetic chain (OKC) dan latihan isometrik terhadap penurunan nyeri pada penderita osteoartrits knee. Penelitian dengan two group pre and post test design. Subjek penelitian  ini merupakan pasien dengan kondisi nyeri lutut osteoarthritis yang berjumlah 40 orang yang terbagi menjadi 2 kelompok sehingga tiap kelompok berjumlah 20 orang. Intervensi dilakukan seminggu 3 kali sebanyak 12 kali latihan. Pengukuran nyeri dilakukan di awal dan akhir setelah seluruh program perlakuan selesai. Alat ukur pada penelitian ini menggunakan VAS. Hasil uji beda pre dan post-test pada kelompok OKC dan isometrik menggunakan uji wilcoxon dan didapatkan hasil p=0,000 (p<0,05). Adapun hasil uji beda post-post kelompok OKC dan isometrik menggunakan uji mann-whitney dan didapatkan hasil p=0,01 (p<0,05). Mean kelompok I : 2 dan mean kelompok II : 1. Latihan open kinetic chain lebih efektif dari pada latihan isometrik terhadap penurunan nyeri penderita osteoartritis lutut.
Aplikasi NMES, IR, serta Terapi Latihan Pada Pasien Post Stroke: Laporan Kasus
Authors: Salsabila Naura , Rahman Farid
Published: January 2024

NMES banyak digunakan untuk pengobatan manajemen nyeri, penguatan otot dan pemulihan sensorimotor. Pasien wanita usia 60 tahun datang ke poli fisioterapi  dengan diagnosa medis Hemiparese sinistra et causa Stroke non Hemoragic. Hemiparese adalah salah satu gangguan yang menonjol akibat stroke yang mempengaruhi aktivitas hidup sehari-hari dan kualitas hidup. Cedera otak seperti stroke menyebabkan kerusakan dan kematian sel-sel neuron. Kerusakan otak diproyeksikan menuju kecacatan pada fungsi motorik, gangguan fungsi kognitif yang mengakibatkan hilangnya kemampuan dalam kinerja fungsional (Nudo, 2007). NMES mengaktifkan sistem sensorik-motor melalui pulsa listrik ke saraf dan otot dan memfasilitasi gerakan sukarela dan penguatan otot. Dalam beberapa penelitian sebelumnya di jelaskan bahwa Stimulasi listrik neuromuskular (NMES) pada ekstremitas dinilai aman dan murah serta berpotensi memfasilitasi pemulihan dalam hal kekuatan otot, keseimbangan berdiri, peningkatan kecepatan berjalan, dan aktivitas sehari-hari. Intervensi yang diberikan  dalam laporan kasus ini berupa NMES ditambah dengan IR serta terapi latihan. Hal yang membedakan artikel ini dengan artikel NMES yang sudah ada sebelumnya adalah penentuan goals yang realistis karena kembali melihat realita keadaan pasien yang tidak memungkinkan untuk mencapai goals pada artikel artikel lain seperti berdiri dan berjalan.
Manajemen Fisioterapi pada Sindrom Obstruksi Pasca Tuberkulosis (SOPT): Laporan Kasus
Authors: Irianto Irianto , Mahardika Agung Satrya , Malik Kasmia , Ishan Lutfiyah Mawaddatul , Syahar Alfi
Published: January 2024

Kondisi adanya obstruksi saluran napas atau Sindrom Obstruksi Pasca Tuberkulosis (SOPT). Pasien tuberkulosis dengan lesi paru minimal bisa menyebabkan kerusakan paru yang terjadi pada saluran pernapasan obstruktif dimana membuat komplikasi terjadi pada sebagian besar penderita tuberkulosis setelah pengobatan dengan gejala sisa paling sering gangguan paru yang bersifat obstruktif. Adapun tujuan dari penelitian ini untuk memberikan gambaran manajemen fisioterapi pada kasus SOPT. Studi ini merupakan laporan kasus, data primer diperoleh melalui autoanamnesis, alloanamnesis, dan pemeriksaan fisik. Pasien pria usia 52 tahun melakukan tindakan fisioterapi dengan keluhan utama berupa sesak napas, batuk dan kecemasaan. Pasien mendapatkan intervensi fisioterapi dalam satu sesi per minggu selama dua minggu. Hasil positif pada penurunan sesak dan batuk juga kecemasaan. Laporan kasus ini menggambarkan presentasi klinis dan manajemen fisioterapi kasus SOPT dimana program fisioterapi yang bersifat individu dan mempentingkan keadaan klinis pasien.
Pengaruh Senam Hipertensi Terhadap Kualitas Hidup Lansia Penderita Hipertensi
Authors: Astuti Dwi Nur , Agustiningsih Luhur Sesanti , Wibisono Irawan , Triyana Triyana
Published: January 2024

Kualitas hidup penderita hipertensi lebih buruk dibandingkan dengan individu yang memiliki tekanan darah normal. Hal tersebut dipengaruhi oleh tekanan darah dan tingkat kesadaran seseorang tersebut. Peningkatan kualitas hidup bisa diperoleh melalui latihan fisik yaitu senam hipertensi dengan tujuan mengurangi stress, meningkatkan rasa percaya diri, serta mengurangi kecemasan dan depresi terkait penyakit yang dialaminya. Penelitian ini bertujuan untuk mngetahui pengaruh senam hipertensi terhadap kualitas hidup lansia penderita hipertensi di Panti wredha Rindang Asih II Bongsari, diharapkan kualitas hidup lansia menjadi lebih baik setelah diberikan senam hipertensi. Desain penelitian yang digunakan bersifat eksperimental dengan rancangan pre-post test one group design. Jumlah sample 22 lansia yang diambil dengan teknik Purposive Sampling. Instrumen pengumpuan data dengan kuesioner WHOQOL-BREF yang diukur sebelum dan sesudah intervensi, yang dilakukan dua kali seminggu selama empat minggu. Analisa data menggunakan Wilcoxon Signed Rank Test dengan α < 0,05. Hasil penelitian menunjukkan 22 responden mengalami peningkatan hasil kualitas hidup dari nilai pre test ke nilai post test dengan nilai mean rank atau rata-rata peningkatan adalah 11.50. Hasil uji Wilcoxon Signed Rank Test didapatkan hasil þ= 0,000 < α=0,05 sehingga Ha diterima yaitu ada perbedaan antara kualitas hidup lansia untuk pre tes dan post test, sehingga dapat disimpulkan ada pengaruh senam hipertensi terhadap peningkatan kualitas hidup pada lansia penderita hipertensi di Panti Wredha Rindang Asih II Bongsari Semarang.
OECD Ülkelerinin Rekabet Performansının Entropi Tabanlı GİA Yöntemiyle Değerlendirilmesi
Authors: IŞIK Fikret
Published: January 2024

Endeksler doğrudan ölçülemeyen fakat sonuçları işletme ve ülkeler için kritik öneme sahip çok boyutlu olguları ölçülebilir hale getiren araçlardır. Endekslerin ürettiği veriler stratejik karar birimlerine önemli ipuçları sağlamaktadır. Ülkeler arası rekabet gücü endeksleri stratejik amaçlara daha etkin ulaşmak için özellikle kaynak tahsisi kararları açısından değerli göstergelerdir. Ülkeler arası rekabet gücü kıyaslamaları daha isabetli sonuçlara ulaşmak amacıyla benzer ülke grupları arasında karşılaştırılarak incelenmektedir. Bu çalışmada Türkiye’nin de dâhil olduğu OECD ülkelerinin Küresel Sürdürülebilir Rekabet Endeksi performansları değerlendirilmiştir. OECD ülkelerinin Solability tarafından hesaplanan 2022 yılı KSRE performans puanları ham veri olarak alınmış, önce entropi yöntemiyle alt endeksler ağırlıklandırılmış daha sonra Gri İlişkisel Analiz yöntemiyle ülkelerin KSRE puanları derecelendirilerek sıralanmıştır. Sürdürülebilir Rekabetçilik Performansı üzerinde en etkili endeksler Sosyal Sermaye Endeksi ve Doğal Sermaye Endeksi; en düşük etkiye sahip endeks ise Yönetişim Endeksi olmuştur. OECD ülkeleri arasında İsveç, İzlanda ve İsviçre en iyi sürdürülebilir rekabetçilik performansına sahip ülke olmuştur. Meksika OECD ülkeleri içinde son sırada yer alırken Türkiye sondan ikinci olmuştur. Sürdürülebilir rekabetçilik performansının yükseltilmesi için Sosyal Sermaye Endeksi ve Doğal Sermaye Endeksi boyutlarına yapılacak yatırımlar daha etkili olacaktır.
Yaratıcı Takım İkliminde Bilge Liderliğin Rolü: Havayolu Kabin Memurları Üzerine Bir Araştırma
Authors: GÖKGÖZ Hakan
Published: January 2024

Günümüzün artan ve giderek karmaşık bir hal alan rekabet ortamında liderlerin, bilge olarak karakterize edilebilecek niteliklere ve özelliklere sahip olmaları, örgüt açısından önem arz etmektedir. Bilge lider, çalışanlarıyla bilgi alışverişinde bulunan, farklı fikir, görüş ve önerileri dikkate alan, çalışanlarıyla etkileşim ve iletişim içinde olan liderdir. Örgütün hedeflerine ulaşmasında çalışanların katkısının büyük olduğunun farkındadır. Bu yüzden çalışanları arasında yaratıcı takım ikliminin oluşması ve gelişmesi için uğraşmaktadır. Bu çalışmada bağlı bulundukları amirlerin bilge liderlik özelliklerine sahip olmalarının, kabin memurlarında yaratıcı takım iklimi oluşmasındaki varlığı ve etkisi araştırılmıştır. Çalışmada kapsamında elde edilen veriler yapısal eşitlik modellemesi test edilmiştir. Sonuca göre bilge liderlik ile yaratıcı takım iklimi arasında pozitif yönde ve anlamlı bir ilişki bulunmuştur. Bilge liderlik değişkenindeki bir puanlık artış, yaratıcı takım iklimi değişkeninde 0.62 puanlık bir artışa yol açmaktadır. Dolayısıyla, çalışmaya katılan kabin memurlarında bilge liderlik algısının yaratıcı takım iklimi üzerinde anlamlı bir etkisinin olduğu bulunmuştur.
Üniversite Özerkliği Üzerine Tarihsel Bir İnceleme
Authors: BUĞDAY Fatma
Published: January 2024

Orta Çağ Avrupası’nda ortaya çıktığı kabul edilen üniversitelerin niteliği ve fonksiyonu yüzyıllar içerisinde dönüşerek devam etmiştir. Üniversitelerin tarihsel gelişim sürecinde ve kültürel mirasında 19. yüzyılda ortaya çıkan Humboldt Üniversitesi modeli önem taşımaktadır. Özerklik üzerine inşa edilen bu model üzerinden modern üniversite dönemine geçilmiştir. Humboldt Üniversitesi modelinde üniversite özerkliği bilimin kamuya yönelik hizmet ve sorumluluğundan dolayı devlet otoritesi kavramı dışında gelişmiştir. Çalışmada, bilgi üretimi ve bilginin toplumsallaştırılması için üniversite özerkliğine neden gereksinim duyulduğu hususunun tarihsel gereksinimler üzerinden incelenmesi ve üniversite kavramını tarihsel gelişim içerisinde analizi amaçlanmıştır. Makale, nitel araştırma yöntemiyle betimsel olarak incelenmiştir. Bu kapsamda iki ana bölüm olarak tasarlanan çalışmanın birinci bölümünde; tarihte üniversite olarak kabul edilebilecek kurumların dönüşümü, özerklik temelinde, literatürde yer alan üç dönem üzerinden incelenmiştir. İkinci bölümde ise üniversite özerkliğinin anlamı, tanımı, kapsamı ve türleri tartışıldıktan sonra üniversite özerkliğine yönelik tutumlar analiz edilmiştir. Makalede şu tespitlere varılmıştır: Orta Çağ dönemi üniversitelerinde bilginin üretilmediği aktarıldığı bir yapılanma ile birlikte kısmi bir kurumsal özerkliğe sahip oldukları görülmektedir. Humboldt Üniversitesi Modeli ile birlikte modern üniversiteye geçiş yapılarak bilginin üretimi önem kazanmıştır. Bu doğrultuda bilgi üretimi için akademik özgürlüğe gereksinim duyulup üniversite özerkliğine vurgu yapılmıştır. Girişimci Üniversite modelinde ise üniversite özerkliğinin iktisadi boyutu olan mali özerkliğin diğer özerklik göstergelerine göre nispeten yükseldiği görülmektedir.
Terapi Healing The Mind Untuk Mengatasi Kondisi Traumatik Pada Anak Dan Remaja Korban Kekerasan Seksual
Authors: Pangesti Nova Ari , Saputri Dita Ayu Nur
Published: January 2024

Kekerasan seksual terhadap anak terus menjadi ancaman yang serius. Dampak kekerasan seksual yang paling urgent adalah anak akan memiliki trauma yang mendalam sehingga dapat mengganggu pertumbuhan dan perkembangan. Anak rentan mengalami gangguan stress pascatrauma (PTSD) yang ditandai dengan keinginan untuk melakukan bunuh diri, kecemasan yang meningkat, gelisah, khawatir akan masa depannya, hingga keinginan menjadi pelaku kekerasan seksual di kemudian hari. Salah satu perawatan non farmakologis pada korban kekerasan seksual yang mengalami trauma adalah terapi Healing the Mind dengan inovasi peneliti menggabungkan art therapy dan spiritual. Tujuan penelitian menganalisis pengaruh terapi Healing The Mind dalam mengatasi traumatic pada anak-anak dan remaja yang mengalami kekerasan seksual. Metode Penelitian ini merupakan jenis penelitian kualitatif yang menerapkan metode eksploratif (exploratory research) dengan menerapkan teknik wawancara mendalam (in-depth interview). Analisa data menggunakan Collaizi. Hasil Ada tiga tema utama yang diidentifikasi dalam penelitian ini, yaitu (1) Kondisi trauma yang dirasakan sebelum diberikan terapi healing the mind yang terdiri dari 3 sub tema (2) Kondisi Trauma yang Dirasakan Sebelum Diberikan Terapi Healing The Mind yang terdiri dari 1 sub tema (3) Efektifitas terapi Healing The Mind yang terdiri dari 4 sub tema. Kesimpulan  Partisipan berjumlah empat orang menyatakan perasaan yang dialami setiap partisipan berbeda setelah peristiwa kekerasan seksual. Terapi penyembuhan pikiran (healing the mind) memiliki kemampuan untuk mengungkapkan emosi yang tidak dapat diungkapkan, mengurangi tingkat trauma, dan menimbulkan rasa tenang pada individu.
Multimodal Hand Hygiene Improvement Strategy Meningkatkan Kepatuhan Cuci Tangan Five Moment
Authors: Maryana Maryana , Anggraini Rima Berti
Published: January 2024

Perawat dapat menjadi sumber infeksi nosokomial di rumah sakit. Kebersihan tangan perawat menjadi hal yang sangat terpenting untuk mengurangi infeksi terkait perawatan, khususnya infeksi nosokomial. Penelitian ini bertujuan untuk mengeksploratif Multimodal Hand Hygiene Improvement Stategy sebagai solusi bagi pengelola PPI dalam meningkatkan kepatuhan cuci tangan perawat sesuai standar di rumah sakit. Metode penelitian ini menggunakan pendekatan pilot study yang bersifat observasional. Ruangan yang digunakan sebagai pilot studi adalah ruangan keperawatan anak yang terdiri dari 12 orang perawat pelaksana, 1 orang dokter spesialis, dan 1 orang dokter asisten. Hasil penelitian menunjukkan bahwa kepatuhan cuci tangan perawat mengalami peningkatan menjadi 80% setelah diterapkan Multimodal Hand Hygiene Improvement Stategy Simpulan, kepatuhan cuci tangan perawat tergolong dalam kategori patuh setelah dilakukan Multimodal Hand Hygiene Improvement Stategy. Disarankan   Multimodal Hand Hygiene Improvement Stategy dapat dijadikan pendekatan untuk meningkatkan kepatuhan Hand Hygiene Five Moment di rumah sakit.
Engineering allosteric inhibition of homoserine dehydrogenase by semi-rational saturation mutagenesis screening
Authors: Liu Xinyang , Liu Jiao , Liu Zhemin , Qiao Qianqian , Ni Xiaomeng , Yang Jinxing , Sun Guannan , Li Fanghe , Zhou Wenjuan , Guo Xuan , Chen Jiuzhou , Jia Shiru , Zheng Yu , Zheng Ping , Sun Jibin
Published: January 2024

Allosteric regulation by pathway products plays a vital role in amino acid metabolism. Homoserine dehydrogenase (HSD), the key enzyme for the biosynthesis of various aspartate family amino acids, is subject to feedback inhibition by l-threonine and l-isoleucine. The desensitized mutants with the potential for amino acid production remain limited. Herein, a semi-rational approach was proposed to relieve the feedback inhibition. HSD from Corynebacterium glutamicum (CgHSD) was first characterized as a homotetramer, and nine conservative sites at the tetramer interface were selected for saturation mutagenesis by structural simulations and sequence analysis. Then, we established a high-throughput screening (HTS) method based on resistance to l-threonine analog and successfully acquired two dominant mutants (I397V and A384D). Compared with the best-ever reported desensitized mutant G378E, both new mutants qualified the engineered strains with higher production of CgHSD-dependent amino acids. The mutant and wild-type enzymes were purified and assessed in the presence or absence of inhibitors. Both purified mutants maintained >90% activity with 10 mM l-threonine or 25 mM l-isoleucine. Moreover, they showed >50% higher specific activities than G378E without inhibitors. This work provides two competitive alternatives for constructing cell factories of CgHSD-related amino acids and derivatives. Moreover, the proposed approach can be applied to engineering other allosteric enzymes in the amino acid synthesis pathway.
Human and animal exposure to newly discovered sand fly viruses, China
Authors: Yao Xiaohui , Yin Qikai , Tian Xiaodong , Zheng Yuke , Li Hongyan , Fu Shihong , Lian Zhengmin , Zhang Yijia , Li Fan , Zhang Weijia , He Ying , Wang Ruichen , Wu Bin , Nie Kai , Xu Songtao , Cheng Jingxia , Li Xiangdong , Wang Huanyu , Liang Guodong
Published: January 2024

Introduction

The Hedi virus (HEDV) and Wuxiang virus (WUXV) are newly discovered Bunyaviruses transmitted by sandflies. The geographical distribution of isolation of these two viruses continues to expand and it has been reported that WUXV causes neurological symptoms and even death in suckling mice. However, little is known about the prevalence of the two viruses in mammalian infections.

Methods

In order to understand the infection status of HEDV and WUXV in humans and animals from regions where the viruses have been isolated, this study used Western blotting to detect the positive rates of HEDV and WUXV IgG antibodies in serum samples from febrile patients, dogs, and chickens in the forementioned regions.

Results

The results showed that of the 29 human serum samples, 17.24% (5/29) tested positive for HEDV, while 68.96% (20/29) were positive for WUXV. In the 31 dog serum samples, 87.10% (27/31) were positive for HEDV and 70.97% (22/31) were positive for WUXV, while in the 36 chicken serum samples, 47.22% (17/36) were positive for HEDV, and 52.78% (19/36) were positive for WUXV.

Discussion

These findings suggest there are widespread infections of HEDV and WUXV in mammals (dogs, chickens) and humans from the regions where these viruses have been isolated. Moreover, the positive rate of HEDV infections was higher in local animals compared to that measured in human specimens. This is the first seroepidemiological study of these two sandfly-transmitted viruses. The findings of the study have practical implications for vector-borne viral infections and related zoonotic infections in China, as well as providing an important reference for studies on the relationship between sandfly-transmitted viruses and zoonotic infections outside of China.
Development and validation of an art-inspired multimodal interactive technology system for a multi-component intervention for older people: a pilot study
Authors: Camurri Antonio , Seminerio Emanuele , Morganti Wanda , Canepa Corrado , Ferrari Nicola , Ghisio Simone , Cera Andrea , Coletta Paolo , Barbagelata Marina , Puleo Gianluca , Nolasco Ilaria , Costantini Claudio , Senesi Barbara , Pilotto Alberto
Published: January 2024

Introduction

The World Health Organization (WHO) acknowledges the presence of a significant body of research on the positive effects of the arts on health, considering a variety of factors including physical well-being, quality of life, and social and community impact. The model that underlies cultural welfare puts the performing arts, visual arts, and cultural heritage at the service of people personal and societal well-being. The potential connections between movements of the body and artistic content have been extensively studied over time, considering movement as a non-verbal language with a universal character.

Methods

This pilot study presents the results of the validation of an innovative multimodal system, the DanzArTe-Emotional Wellbeing Technology, designed to support active and participative experience of older people providing physical and cognitive activation through a full-body physical interaction with a traditional visual work of art of religious subject. DanzArTe supports a replicable treatment protocol for multidimensional frailty, administered through a low cost and scalable technological platform capable of generating real-time visual and auditory feedback (interactive sonification) from the automated analysis of individual as well as joint movement expressive qualities. The study involved 45 participants, 23 of whom participated in the DanzArTe program and 22 who were included in the control group.

Results

The two groups were similar in terms of age (p = 0.465) and gender (p = 0.683). The results showed that the DanzArTe program had a positive impact on participants' self-perceived psychological health and well-being (Mean Psychological General Well-Being Index—Short T1 = 19.6 ± 4.3 Vs. T2 = 20.8 ± 4.9; p = 0.029). The same trend was not observed in the control group (p = 0.389).

Discussion

The findings suggest that such programs may have a significant impact particularly on the mental and social well-being of older adults and could be a valuable tool for promoting healthy aging and improving quality of life.
The effect of pituitrin on postoperative outcomes in patients with pulmonary hypertension undergoing cardiac surgery: a study protocol for a randomized controlled trial
Authors: Kong Lingchen , Lv Meng , Qiao Chang-long , Sun Xia-xuan , Du Wen-ya , Li Quan
Published: January 2024

Background

The vasoplegic syndrome is one of the major consequences of cardiac surgery. If pulmonary hypertension is additionally involved with vasoplegic syndrome, circulation management becomes much more complicated. According to previous studies, pituitrin (a substitute for vasopressin, which contains vasopressin and oxytocin) not only constricts systemic circulation vessels and increases systemic circulation pressure but also likely decreases pulmonary artery pressure and pulmonary vascular resistance. The aim of this study is to investigate whether pituitrin is beneficial for the postoperative outcomes in patients with pulmonary hypertension undergoing cardiac surgery.

Methods and analysis

The randomized controlled trial will include an intervention group continuously infused with 0.04 U/(kg h) of pituitrin and a control group. Adult patients with pulmonary hypertension undergoing elective cardiac surgery will be included in this study. Patients who meet the conditions and give their consent will be randomly assigned to the intervention group or the control group. The primary outcome is the composite endpoint of all-cause mortality within 30 days after surgery or common complications after cardiac surgery. Secondary outcomes include the incidence of other postoperative complications, length of hospital stay, and so on.

Discussion

Pituitrin constricts systemic circulation vessels, increases systemic circulation pressure, and may reduce pulmonary artery pressure and pulmonary vascular resistance, which makes it a potentially promising vasopressor during the perioperative period in patients with pulmonary hypertension. Therefore, evidence from randomized controlled trials is necessary to elucidate whether pituitrin influences outcomes in patients with pulmonary hypertension following cardiac surgery.
Navigating the AI frontiers in cardiovascular research: a bibliometric exploration and topic modeling
Authors: Shiferaw Kirubel Biruk , Wali Payam , Waltemath Dagmar , Zeleke Atinkut Alamirrew
Published: January 2024

Artificial intelligence (AI) has emerged as a promising field in cardiovascular disease (CVD) research, offering innovative approaches to enhance diagnosis, treatment, and patient outcomes. In this study, we conducted bibliometric analysis combined with topic modeling to provide a comprehensive overview of the AI research landscape in CVD. Our analysis included 23,846 studies from Web of Science and PubMed, capturing the latest advancements and trends in this rapidly evolving field. By employing LDA (Latent Dirichlet Allocation) we identified key research themes, trends, and collaborations within the AI-CVD domain.

The findings revealed the exponential growth of AI-related research in CVD, underscoring its immense potential to revolutionize cardiovascular healthcare. The annual scientific publication of machine learning papers in CVD increases continuously and significantly since 2016, with an overall annual growth rate of 22.8%. Almost half (46.2%) of the growth happened in the last 5 years. USA, China, India, UK and Korea were the top five productive countries in number of publications. UK, Germany and Australia were the most collaborative countries with a multiple country publication (MCP) value of 42.8%, 40.3% and 40.0% respectively. We observed the emergence of twenty-two distinct research topics, including “stroke and robotic rehabilitation therapy,” “robotic-assisted cardiac surgery,” and “cardiac image analysis,” which persisted as major topics throughout the years. Other topics, such as “retinal image analysis and CVD” and “biomarker and wearable signal analyses,” have recently emerged as dominant areas of research in cardiovascular medicine.

Convolutional neural network appears to be the most mentioned algorithm followed by LSTM (Long Short-Term Memory) and KNN (K-Nearest Neighbours). This indicates that the future direction of AI cardiovascular research is predominantly directing toward neural networks and image analysis.

As AI continues to shape the landscape of CVD research, our study serves as a comprehensive guide for researchers, practitioners, and policymakers, providing valuable insights into the current state of AI in CVD research. This study offers a deep understanding of research trends and paves the way for future directions to maximiz the potential of AI to effectively combat cardiovascular diseases.
Relationship of weight change patterns from young to middle adulthood with incident rheumatoid arthritis and osteoarthritis: a retrospective cohort study
Authors: Nan Kai , Zhang Ming , Hu Shouye , Shao Xiaolong , Liu Lin , Zhi Yang , Xu Peng
Published: January 2024

Background

The relationship between weight change patterns and arthritis onset, specifically rheumatoid arthritis (RA) and osteoarthritis (OA), is unclear. We examined the association between weight changes from young adulthood to midlife and arthritis onset.

Methods

Using data from NHANES 1999–2018, participants with self-reported arthritis were selected. Age at diagnosis determined arthritis onset. Weight change patterns were based on BMI at age 25 and 10 years before the survey. Patterns were categorized as stable non-obese, non-obese to obese, obese to non-obese, and stable obese. Cox regression models and restricted cubic spline (RCS) analysis were employed, calculating hazard ratios (HRs) and 95% confidence intervals (CIs) considering covariates.

Results

Out of 20,859 participants (male 11,017, 52.82%), 4922 developed arthritis over a mean 8.66-year follow-up. Compared to stable non-obese individuals, the HRs for arthritis were 1.55 (95% CI=1.45 to 1.66, P < 0.0001) for non-obese to obese and 1.74 (95% CI=1.56 to 1.95, P < 0.0001) for stable obese. Those gaining 10-20 kg had a HR of 1.33 (95% CI=1.22 to 1.46, P < 0.0001), and gains >20 kg had a HR of 1.56 (95% CI=1.42 to 1.71, P < 0.0001), compared to stable weight (change within 2.5 kg). Identical results observed for OA and RA. RCS showed a nonlinear relationship between weight change and arthritis (all P < 0.01).

Conclusions

Stable obesity and weight gain during adulthood increase arthritis risk. Maintaining a non-obese weight throughout adult years might reduce arthritis risk in later life.
Association between the metabolic score for insulin resistance (METS-IR) and arterial stiffness among health check-up population in Japan: a retrospective cross-sectional study
Authors: Liu Gailing
Published: January 2024

Aim

This study examined the association between the metabolic score for insulin resistance (METS-IR), a novel surrogate indicator of insulin resistance, and brachial-ankle pulse wave velocity (baPWV) in Japanese health check participants.

Methods

A cross-sectional research was conducted involving individuals in a program of medical health screening at the Medical Health Check-up Center in Japan. The study assessed the connection between METS-IR and baPWV in 912 participants who joined the program between 3/1/2004 and 12/31/2012. Serum laboratory tests and lifestyle surveys were the covariates. Multivariate linear regression analysis and subgroup analyses were performed.

Results

912 participants were involved in this cross-sectional study. Adjusted for age, sex, BMI, AST, UA, HDL, eGFR, ankle-brachial index (ABI), alcohol consumption, and smoking status, multivariate linear regression analysis showed that METS-IR and baPWV showed a significant positive association (adjusted β=15.3, 95% confidence interval (CI): 6.61~23.98) with METS-IR as a continuous variable. When converting METS-IR to quartile categorical variables, higher quartile METS-IR indices had longer baPWV (Q3 vs Q1, β=86.14, 95% CI: 23.45~148.83; Q4 vs Q1, β=125.41, 95% CI: 39.99~210.84). In subgroup analysis, METS-IR was associated with baPWV in people with eGFR > 60 ml/min (adjusted β= 14.44, 95% CI: 5.61~23.26, P=0.001), none or light alcohol consumption (adjusted β=16.92, 95% CI: 6.85~27, P=0.001), non-smokers (adjusted β=15.48, 95% CI: 5.86~25.1, P=0.002), non-regular exercisers(adjusted β=17.34, 95% CI: 8.03~26.65, P<0.001), non-fatty liver (adjusted β=17.65, 95% CI: 5.92~29.39, P=0.003), and non-hypertensive (adjusted β=16.13, 95% CI:8.45~23.8, P<0.001).

Conclusion

METS-IR are remarkably associated with baPWV among health check-up participants in Gifu, Japan. As a simple, easily calculated predictor of arterial stiffness, METS-IR could be considered for primary care as a monitoring tool to identify people with high risk of cardiovascular disease in order to intervene early on risk factors. Future prospective, large-sample researches are still needed to confirm this.
Probabilistic prediction of wind power based on improved Bayesian neural network
Authors: Deng Zhiguang , Zhang Xu , Li Zhengming , Yang Jinghua , Lv Xin , Wu Qian , Zhu Biwei
Published: January 2024

Deterministic wind power prediction can be used for long time-scale optimization of power dispatching systems, but the probability and fluctuation range of prediction results cannot be calculated. A Bayesian LSTM neural network (BNN-LSTM) is constructed based on Bayesian networks by placing a priori distributions on top of the LSTM network layer weight parameters. First, the temporal convolutional neural network (TCNN) is used to process the historical time-series data for wind power prediction, which is used to extract the correlation features of the time-series data and learn the trend changes of the time-series data. Then, the mutual information entropy method is used to analyze the meteorological dataset of wind power, which is used to eliminate the variables with small correlation and reduce the dimension of the meteorological dataset, so as to simplify the overall structure of the prediction model. At the same time, the Embedding structure is used to learn the temporal classification features of wind power. Finally, the time series data processed by TCNN, the meteorological data after dimensionality reduction, and the time classification feature data are fed into the BNN-LSTM prediction model together. Compared with a Bayesian neural network, continuous interval method, and Temporal Fusion Transformer (TFT), which is one of the most advanced time series prediction networks, the improved BNN-LSTM can respond more accurately to wind power fluctuations with better prediction results. The comprehensive index of probability prediction of pinball loss is smaller than those of the other three methods by 53.2%, 24.4%, and 11.3%, and the Winkler index is 3.5 %, 34.6 %, and 8.2 % smaller, respectively.
A hybrid RBF neural network based model for day-ahead prediction of photovoltaic plant power output
Authors: Zhang Qipei , Tang Ningkai , Lu Jixiang , Wang Wei , Wu Lin , Kuang Wenteng
Published: January 2024

Renewable energy resources like solar power contribute greatly to decreasing emissions of carbon dioxide and substituting generators fueled by fossil fuels. Due to the unpredictable and intermittent nature of solar power production as a result of solar radiance and other weather conditions, it is very difficult to integrate solar power into conventional power systems operation economically in a reliable manner, which would emphasize demand for accurate prediction techniques. The study proposes and applies a revised radial basis function neural network (RBFNN) scheme to predict the short-term power output of photovoltaic plant in a day-ahead prediction manner. In the proposed method, the linear as well as non-linear variables in the RBFNN scheme are efficiently trained using the whale optimization algorithm to speed the convergence of prediction results. A nonlinear benchmark function has also been used to validate the suggested scheme, which was also used in predicting the power output of solar energy for a well-designed experiment. A comparison study case generating different outcomes shows that the suggested approach could provide a higher level of prediction precision than other methods in similar scenarios, which suggests the proposed method can be used as a more suitable tool to deal such solar energy forecasting issues.
Effects of large dams on the aquatic food web along a coastal stream with high sediment loads
Authors: Roussel Jean-Marc , Fraisse Stéphane , Dézerald Olivier , Fovet Ophélie , Pannard Alexandrine , Rodriguez-Perez Hector , Crave Alain , Gorzerino Caroline , Poupelin Maxime , Forget Guillaume , Huteau Dominique , Thomas Alban , Chevé Manuel , Soissons Laura , Piscart Christophe
Published: January 2024

The contribution of two basal energy sources – detrital organic matter and primary producers – as part of aquatic food webs varies typically along river continua. A host of barriers to river flow increase the water residence time and sediment and nutrient retention in reservoirs worldwide, and potentially alter the balance between detritus-based and algae-based energy pathways in the downstream food webs. We explored this issue on the Sélune River (Normandy, France), a small coastal stream that drains an agricultural catchment with high sediment runoff. Seasonal measurements of the following parameters were compared upstream and downstream of the reservoirs of two large dams (16 m and 36 m high): sediment fluxes, nutrient and chlorophyll a concentrations, algal communities in the epilithic biofilm (taxonomic composition, biomass and growth), and benthic invertebrate communities (abundance and trophic guild structure). As anticipated, annual sediment fluxes were much lower downstream of the reservoirs, where significant decreases in water turbidity, phosphate and silicate concentrations were recorded. A higher chlorophyll a concentration in water and a higher contribution of pelagic algae taxa to the photosynthetic biofilm suggested drifting and deposition of reservoir-borne phytoplankton downriver. Photosynthetic biofilm growth was higher downstream of the reservoirs in spring and fall, and so was the abundance of herbivores in the invertebrate community, notably scrapers and algae eaters. Energy pathways within riverine food webs were traced using stable isotope analyses of carbon (C) and nitrogen in the tissues of aquatic consumers (invertebrates and fish). Mixing models revealed a discontinuity in the origin of the C entering the food webs along the river continuum, confirming a greater contribution of algal C to aquatic consumers downstream of the reservoirs. These results illustrate mechanisms whereby large reservoirs can modulate C flow in food webs along a small coastal river with high sediment loads, and make it possible to anticipate the effects of dam removal on the future river ecosystem.
Frequencies of activated T cell populations increase in breast milk of HCMV-seropositive mothers during local HCMV reactivation
Authors: Lazar Katrin , Pawelec Graham , Goelz Rangmar , Hamprecht Klaus , Wistuba-Hamprecht Kilian
Published: January 2024

Background

Human cytomegalovirus (HCMV) can reactivate in the mammary gland during lactation and is shed into breast milk of nearly every HCMV-IgG-seropositive mother of a preterm infant. Dynamics of breast milk leukocytes during lactation, as well as blood leukocytes and the comparison between both in the context of HCMV reactivation is not well understood.

Methods

Here, we present the BlooMil study that aimed at comparing changes of immune cells in blood and breast milk from HCMV-seropositive- vs -seronegative mothers, collected at four time ranges up to two months post-partum. Viral load was monitored by qPCR and nested PCR. Multiparameter flow cytometry was used to identify leukocyte subsets.

Results

CD3+ T cell frequencies were found to increase rapidly in HCMV-seropositive mothers’ milk, while they remained unchanged in matched blood samples, and in both blood and breast milk of HCMV-seronegatives. The activation marker HLA-DR was more strongly expressed on CD4+ and CD8+ T cells in all breast milk samples than matched blood samples, but HCMV-seropositive mothers displayed a significant increase of HLA-DR+ CD4+ and HLA-DR+ CD8+ T cells during lactation. The CD4+/CD8+ T cell ratio was lower in breast milk of HCMV-seropositive mothers than in the blood. HCMV-specific CD8+ T cell frequencies (recognizing pp65 or IE1) were elevated in breast milk relative to blood, which might be due to clonal expansion of these cells during local HCMV reactivation. Breast milk contained very low frequencies of naïve T cells with no significant differences depending on serostatus.

Conclusion

Taken together, we conclude that the distribution of breast milk leukocyte populations is different from blood leukocytes and may contribute to the decrease of breast milk viral load in the late phase of HCMV reactivation in the mammary gland.
A comprehensive battery of flow cytometric immunoassays for the in vitro testing of chemical effects in human blood cells
Authors: Pierzchalski Arkadiusz , Zenclussen Ana C. , Herberth Gunda
Published: January 2024

Background

There is a growing need for immunological assays to test toxic and modulatory effects of chemicals. The assays should be easy to use, reproducible and superior to cell line-based assays. We have therefore developed a comprehensive portfolio of assays based on primary human blood cells that are suitable for testing chemical effects.

Methods

The flow cytometry-based assays were designed to target a wide range of human peripheral blood mononuclear cells and whole blood, including T cells, NK cells, B cells, basophils and innate-like T cells such as γδT, MAIT and NKT cells. We have selected a set of activation markers for each immune cell, e.g: CD154 (T cells), CD137, CD107a (NK cells), CD63 (basophils), CD69, CD83 (B cells), CD69, IFN-γ (MAIT cells) and we selected cell specific stimuli: aCD3 antibodies (T cells); E. coli and cytokines IL-12/15/18 (MAIT cells); CpG ODN2006, R848 or aCD40 antibodies (B cells), fMLP or aFcϵR1 (basophils) or K562 cells (NK cells).

Results

By selecting immune cell-specific markers and cell-specific stimuli, we were able to induce particular immune responses from the targeted immune cells. For example, the response to stimulation with anti-CD3 antibodies was in 36.8% of CD107a+CD8+ cells. Cytokine stimulation induced the production of IFN-γ in 30% of MAIT cells. After stimulation with E. coli, around 50% of MAIT cells produced TNF. About 40% of basophils responded to aFcƐR1 stimulation. Similar activation ranges were achieved in K562-stimulated NK cells.

Conclusion

Our test portfolio covers the most relevant immune cells present in human blood, providing a solid basis for in vitro toxicity and immunomodulatory testing of chemicals. By using human blood, the natural composition of cells found in the blood can be determined and the effects of chemicals can be detected at the cellular level.