bluuebunny/crossref_metadata_2025 huggingface dataset:
Analytical possibilities of determining aristolochic acid I using amperometric biosensors and polarizing fluorescent immunoanalysis
Authors:   Kazan (Privolzhsky) Federal University, Beilinson R. M. ,   A.M. Butlerov Chemical Institute, Lopatko N. Yu. ,   Kazan (Privolzhsky) Federal University,   A.M. Butlerov Chemical Institute, Korolkova K. A. ,   Kazan (Privolzhsky) Federal University,   A.M. Butlerov Chemical Institute, Medyantseva E. P. ,   Kazan (Privolzhsky) Federal University,   A.M. Butlerov Chemical Institute, Eremin S. A. ,   Lomonosov Moscow State University, Muhametova L. I. ,   Lomonosov Moscow State University, Shanin I. A.
Published: May 2024

Tyrosinase amperometric biosensors based on graphite printed electrodes modified with reduced graphene oxide (RGO), multi-walled carbon nanotubes (MWNTs), and a nanocomposite based on RGO and silver nanoparticles (Ag NPs), as well as a variant of polarization fluorescence immunoanalysis (PFIA) using a tracer based on fluorescein-5(6)-carboxamidohexanoic acid for determining aristolochic acid Ⅰ (AA) were developed. It has been found that AA Ⅰ is a tyrosinase inhibitor in the concentration range of 1×10–10–1×10–8 M, with LOD of 7×10–11 M. Modifying the electrode surface by RGO and MWCNTs improved the analytical characteristics of the biosensor; analyzed concentration range increased to 1×10–11–1×10–6 M for RGO and 1×10–10–1×10–6 M for MWCNTs. The correlation coefficient and LOD were 0.9828 and 8×10–12 M, and 0.9859 and 5×10–11 M in the case of the biosensors modified with RGO and MWCNT, respectively. A variant of a competitive polarization fluorescent immunoanalysis was developed, which permitted determining AA Ⅰ in the concentration range of 1×10–11–1×10–7 M with LOD of 7×10–12 M. The concentration of antibodies was 1 mg/ml, the time of incubation of the immune complex tracer-antibody was 5 min. Methods for determining AA Ⅰ were tested using samples of herbal preparations, in roots, leaves, stems of European hoof, as well as in crops grown together with hoof.
Sample preparation of blue ballpoint pen ink for determining the composition of color components by RP HPLC method
Authors:   St. Petersburg State University, Institute of Chemistry, Kechin A. A. , Kravchenko A. V. ,   St. Petersburg State University, Institute of Chemistry, Kartsova L. A. ,   St. Petersburg State University, Institute of Chemistry
Published: May 2024

The paper presents various theoretical and practical aspects of sample preparation of blue ballpoint pen ink applied on paper for subsequent quantitative analysis using reverse-phase high-performance liquid chromatography (RP HPLC). Estimation of analytical characteristics of a variant of cutting out a paper fragment containing applied ink for reproducibility of analysis results is discussed; composition of the extraction system and extraction conditions were evaluated. Qualitative and quantitative characterization of the extracts was performed by RP HPLC with the diode array detection. Using a scalpel as a tool for cutting out sample fragments and an acidified mixture of solvents as an extractant with performing extraction in an ultrasonic bath at the room temperature for 2 minutes is recommended. It was shown that the influence of different degree of pressure on a ballpoint pen when applying ink to the paper can be minimized by using normalized peak areas of individual dyes. The proposed sample preparation conditions were tested when studying the processes of changes in the composition of applied ink during artificial aging under the influence of constant ultraviolet (UV) irradiation.
The use of statistical methods of data analysis in identifying significant parameters to improve the efficiency of production system management
Authors: Speshilova Natalia , Speshilov Evgeny , Tsykalo Kristina
Published: May 2024

In modern realities, the urgent question is how enterprises can remain competitive in their production niche. The key areas in this context are cost reduction and evidence-based cost management while maintaining high quality products. The cost of production is an important economic category that characterizes the sum of all the costs of an enterprise for its production and sale, described by a multidimensional system of indicators. In this regard, it is necessary to identify a list of factors in each specific case, analyze their contribution to the change in cost and select from them the most significant parameters that affect the result of production. Without such actions, it is impossible to search for potential opportunities, reserves and competent cost management. One of the most formalized methods of analysis is the implementation of an economic and mathematical approach based on statistical data with the construction of correlation and regression models. It allows not only to identify the factors influencing the formation of cost, predict the outcome of the activity, but also helps to determine the reserves for its reduction. The purpose of the work is to search for potential opportunities to reduce the amount of production costs, develop measures aimed at forming strategies for the company's activities in order to improve the efficiency of management of the production system. MS Excel and the Statistica software package from StatSoft were used as tools for data analysis, as well as for conducting correlation and regression analysis and building econometric models. For the studied regional enterprise, a forecast of changes in the cost of production has been built. Based on the results of the obtained correlation and regression model, significant factors have been identified that have the greatest impact on cost formation and act as controlled variables. With this in mind, a set of specific measures has been formulated aimed at reducing the cost of production, as well as improving the efficiency of the enterprise and its competitiveness. Among the most significant are: rationing of labor, economical use of materials, as well as optimization of logistics to the client. The applied technique can be implemented for a similar production system.
TELEFISIOTERAPIA PARA O TRATAMENTO DA DOR NO MEMBRO FANTASMA: UM ESTUDO QUASI-EXPERIMENTAL
Authors: Luz Soraia Cristina Tonon da , Souza Juliana Barcellos de , Piazza Amanda de Aguiar , Medeiros Amanda Borges , Vieira Amábile Catarina
Published: May 2024

Justificativa e objetivo: A dor fantasma (DF) é uma complicação comum da amputação de extremidades, com prevalência entre 41% e 46% dos casos. Apesar de sua fisiopatologia incerta, evidências sugerem mecanismos  multifatoriais para explicar o fenômeno doloroso, que afeta diretamente a qualidade de vida do indivíduo. O objetivo do estudo foi analisar o efeito de um protocolo de telefisioterapia para DF na qualidade de vida, intensidade e percepção da dor de indivíduos amputados de extremidades. Método: Estudo quasi-experimental com abordagem quali-quantitativa, com amostra composta por nove indivíduos. Os instrumentos utilizados foram o Questionário de Dor de McGill, a Escala Verbal de Dor (EVD), o Short-Form Health Survey (SF-36) e ficha de avaliação elaborada no formato de entrevista, todos aplicados antes e após o protocolo de tratamento. A intervenção constituiu de uma adaptação ao protocolo de Imagens Motoras Graduadas (IMG); realizado de forma on-line pela plataforma GoogleMeet. A análise quantitativa se deu através do teste de Wilcoxon pareado para variáveis não paramétricas e correlação de Pearson. A abordagem qualitativa foi analisada pelo método de análise de conteúdo. Resultados: Não houve diferença significativa nos parâmetros de qualidade de vida e intensidade da dor pré e pós tratamento, porém houve diminuição significativa da percepção de dor total e nos componentes emocionais, o que também foi evidenciado nos relatos qualitativos. Conclusão: O protocolo adaptado de IMG diminuiu significativamente a percepção de dor, especialmente no aspecto emocional, sem resultados significativos na intensidade da dor e qualidade de vida dos indivíduos.
ENHANCEMENT AND OPTIMIZATION OF THERMOHYDRAULIC CHARACTERISTICS OF A HEAT EXCHANGER WITH THREE FLUIDS USED IN SUSTAINABLE HEATING APPLICATIONS
Authors: Bargah Vikas , Mishra Sudhansu S. , Almasri Belal , Mohapatra Taraprasad
Published: May 2024

An experimental investigation of the thermohydraulic and energetic performance of a heat exchanger with three fluids
(HETF) was carried out in the present work. The HETF comprises two straight tubes and a helical tube inserted in
between the outermost and the innermost tube for better heat transfer. The present heat exchanger is projected for the
heating of two fluids (TF2: normal water and TF3: air) simultaneously by another fluid TF1 (hot water). The present
investigation is carried out with three levels of TF1 flow rate (i.e., 100, 200, and 300 LPH), three levels of TF2 flow
rate (i.e., 50, 100, and 150 LPH), three levels of TF3 flow velocity (i.e., 1, 2, and 3 m/s), and two levels of TF1 inlet
temperature (i.e., 60°С and 80°С). A coupled Taguchi-Grey relational analysis is employed to determine the optimum
combination of control parameters to predict optimized overall performances of the HETF. The outcomes show that
the TF1 inlet temperature has significant influence on the JF factor, exergy efficiency, and sustainability index with
a contribution of 41.29%, 63.06%, and 60.66%, respectively. The optimized performance of the HETF is predicted at
60°С TF1 inlet temperature, 100 LPH TF1 flow rate, 150 LPH TF2 flow rate, and 3 m/s TF3 velocity and the same
result got confirmed with significant enhancement in Grey relational grade of 32.9%.
Medicinal Potential of the Giant Puffball Mushroom Calvatia gigantea (Agaricomycetes): A Review
Authors: Cicha-Jeleń Małgorzata , Muszynska Bozena , Kala Katarzyna , Sulkowska-Ziaja Katarzyna
Published: May 2024

Calvatia gigantea, commonly known as the giant puffball mushroom, has traditionally been regarded as a significant edible and medicinal species due to its wide spectrum of bioactive compounds and its health-promoting properties. This study aims to systematize the knowledge on the nutritional value and therapeutic potential of C. gigantea, highlighting its role in traditional and contemporary medicine. The mushroom is recognized for its nutritional content, including easily digestible protein, carbohydrates, fiber, phenolic compounds, vitamins, and minerals, while being low in calories, cholesterol, and sodium. Furthermore, C. gigantea exhibits a range of biological effects, such as antioxidant, anticancer, antimicrobial, antidiabetic, and wound-healing properties, attributed to its diverse chemical composition that includes unsaturated fatty acids, free amino acids, polysaccharides, and bioactive metabolites.
Does royal jelly have a reproductive effect on progesterone-treated goats?
Authors:   Universidad Nacional Autónoma de México, Alvarez Ramírez Lorenzo , Sánchez Cervantes Alejandra
Published: May 2024

Introduction: Some methods for inducing/synchronizing oestrus and ovulation in goats require the injection of equine chorionic gonadotrophin (eCG). The objective of this study was to determine whether royal jelly (RJ) can be a subs-titute for eCG in CIDR-treated dairy goats. Methods: Sixty-six primiparous goats received the CIDR device for twelve days in April. The eCG group (n=22) was injec-ted with 200 IU of the gonadotropin when the intravaginal device was removed; the RJ group (n=22) received a daily intramuscular injection of 1 g of RJ during the period of CIDR treatment; the CIDR group (n=22) did not receive any additional treatment. A fourth group (Con, n=20) was used as control and received no treat-ment at all. Oestrus response, ovulation and fertility were recorded. Results: The oestrus percentage was higher in eCG (95%, p<0.05) than in the remaining groups (RJ, 41%; CIDR, 36% and Con, 0%) and occurred at shorter intervals (eCG, 35±2.3; RJ, 66±4.6; CIDR, 59±2; h±se; p<0.05). Ovulation was higher in eCG (91%, p<0.05), and RJ and CIDR were not different between them (50% vs 41%, p>0.05). No ovula-tion was recorded in the Con group. The percentage of fertile and birthing animals after 148-158 days was greater in the eCG (91%, p<0.05) compared to other groups (RJ, 36%; CIDR, 36%, Con, 0%), whereas it was not different between the RJ, CIDR and Con groups (p>0.05). Conclusion: There was no evidence of a reproductive effect of RJ on progesterone-treated goats.
Efecto de algunos factores del modelo productivo y las fases lunares sobre la duración de la gestación y sexo de la cría bovina
Authors: Serrano-Díaz Gabriel , Villa-Mesa Orlando ,   Universidad Cooperativa de Colombia
Published: May 2024

Introducción:en elámbito ganadero es usual atribuir el sexo de las crías que nacen a la influencia de algunos factores externos y externos al animal. Este trabajo se ocupa de explorar y sustentar alguna evidencia sobre la posibilidad de que estos factores tengan una asociacion real sobre el sexo de las crías. Metodología:se utilizaron los datos registrados a través de la práctica profesional privada por uno de los au-tores durante 16 años (2000-2015) en 23 fincas correspondientes a 18.124 nacimientos (1.108 con registro de servicio). Se utilizaron los programas Excel y Panacea 2. Se realizaron test de significancia utilizando análisis de varianza de una vía y pruebas de chi-cuadrado en tablas de contingencia. Resultados: el porcentaje de hembras nacidas fue menor y hubo una finca con alta predominancia de la pro-porción de estas. Se constató la marcada estacionalidad de nacimientos a través del año. No se observaron diferencias en las proporciones de hembras y machos nacidos inherentes al sistema de producción o la época del año del nacimiento. Se observó significancia para un mayor porcentaje de nacimientos en la fase lunar creciente, y en esta misma, mayor proporción de machos en los nacimientos. Los días de la lunación al naci-miento no mostraron diferencias en las cantidades de nacimientos y proporciones por sexo. No se observaron diferencias en las proporciones de concepciones por sexo debidas a las fases lunares o días de la lunación en el momento de la concepción. Para la duración de la gestación, se observó significancia en las diferencias determinadas por los factores “finca” y “sistema de producción”; no se observó influencia del sexo de la cría que se estaba gestando ni de las variables mes, fase lunar y día de la lunación en los que ocurrió tanto la concepción como el parto. Conclusiones: el porcentaje de machos concebidos y nacidos es ligeramente superior al de hembras; ninguno de los factores analizados puede ser usado para incrementar el porcentaje de concepciones de uno u otro sexo, pero si es notorio un incremento tanto en la proporción total de nacimientos como de machos en los nacimientos registrados en la fase lunar creciente.
Evaluación comparativa de desempeño zootécnico y grado de emplume en dos líneas genéticas de pollo de engorde comercial
Authors:   Universidad Cooperativa de Colombia, Cotamo-López Ariel Leonardo , Gamboa-González Nidia Fernanda , Suárez-Gómez Nixon Ali
Published: May 2024

Los programas de mejoramiento genético en pollos de engorde se actualizan con base en parámetros de interés como son: desempeño zootécnico, rendimiento y calidad de la canal, así como la viabilidad económica. Por tanto, el desarrollo de nuevas líneas genéticas que respondan positivamente a los desafíos productivos de la industria son uno de los mayores retos encontrados en la actualidad. El objetivo de este trabajo fue evaluar el rendimiento zootécnico y grado de emplume de las aves durante su ciclo productivo. Se usaron 2.400 pollos de engorde machos y hembras, distribuidos en bloques aleatorizados en un sistema factorial 2 x 2 (sexo x línea), siendo dos sexos y dos líneas genéticas (línea A x línea B) con 12 repeticiones por tratamiento. Las aves fueron distribuidas en 48 corrales de 2,25 m x 1,50 m, con una ocupación inicial de 50 aves por corral (14,8 aves/m2), el programa de alimentación y de luz fue establecido por la empresa. Semanalmente se evaluaron los parámetros de desempeño zootécnicos y al día 41 se evaluó el grado de emplume. Los datos se analizaron mediante la prueba estadística de T student y U de Mann Whitney en datos que no presentaron distribución normal, usando el programa IMB SPSS 25. No se observó diferencias significativas entre las líneas genéticas evaluadas para las variables de peso corporal, consumo de alimento, conversión alimenticia, ganancia de peso y eficiencia al día 41 de edad. Con respecto al grado de emplume las hembras de la línea B presentaron un mayor grado de emplume que los demás tratamientos.
Análisis bacteriológico y composicional de la leche bovina de cuatro municipios del departamento de Putumayo, Colombia
Authors:   Universidad de Pamplona, Flórez Delgado Dixon Fabián , Cely Leal Dubel ,   Universidad de Pamplona, González Sthefany Daniela ,   Universidad de Pamplona, Niño Vera Lady Johana ,   Universidad de Pamplona, Robledo Palomino Yoicy
Published: May 2024

La calidad bacteriológica y composicional de la leche cruda bovina tiene implicaciones económicas y sanita-rias muy importantes para el desarrollo agropecuario del país. El objetivo de esta investigación fue analizar bacteriológica y composicionalmente la leche bovina cruda proveniente de sistemas de producción ganaderos doble propósito de los municipios de Puerto Asís, Villagarzón, Orito y Valle del Guamuez en el departamento de Putumayo. Para ello, se empleó un muestreo intencional en 250 predios ganaderos obteniendo de esta manera 854 muestras de leche. Se analizaron los parámetros de células somáticas (cs/mL), recuento de bacterias mesófilas aerobias (UFC/mL), grasa, proteína, lactosa y sólidos totales. Los datos obtenidos fueron sujetos a análisis de varianza con significancia del 5%. Se evidenció diferencia para los parámetros de grasa y sólidos totales entre los municipios. Al analizar los resultados respecto a la Norma Técnica Colombiana 399 para leche cruda se cumplen los parámetros de células somáticas, bacterias mesófilas, proteína, lactosa y sólidos totales se cumplen en los cuatro municipios. Por su parte, el parámetro de grasa se cumple en Puerto Asís y Valle del Guamuez.
The case of Lyme borreliosis in the Astrakhan region
Authors: Cherenova L. P. , Bogdalova A. R. , Khok M. M. , Cherenov I. V.
Published: May 2024

The Astrakhan region is not a natural focus of Lyme borreliosis, however, the geographical proximity of endemic territories contributes to the migration of this disease. In 2019, for the first time, a case of Lyme disease was registered in a resident of Astrakhan, who was on vacation in Kislovodsk. In 2022, 3 cases of Lyme disease were again reported in residents of Astrakhan who traveled to regions endemic for tick-borne borreliosis. In 2022, Lyme disease was detected in a resident of Astrakhan who did not travel to the Lyme-borreliosis-endemic regions.
On the issue of blood circulation features in the fetoplacental complex in pregnant women with thyroid diseases
Authors: Alsabekova M. K. , Sinchikhin S. P. , Karimova D. Yu. , Sinchikhina E. S.
Published: May 2024

Studying the issues of pregnancy management with extragenital diseases is a relevant and important task of modern obstetrics. The issue of the influence of thyroid diseases on the formation and function of the placenta remains not fully studied. Purpose of the study: to study the characteristics of the uteroplacental and fetal placental circulation in pregnant women with certain diseases of the thyroid gland. Material and research methods. 170 pregnant women were under observation. The 1st group (64 people) was formed by patients with autoimmune thyroiditis (AIT), the 2nd group (76 people) was formed by patients with diffuse toxic goiter (DTG), the 3rd (control) group was made up of 30 women with normal pregnancy. The study of feto-placental blood circulation was carried out by analyzing blood flow velocity curves (BSV) in the uterine arteries and in the umbilical cord artery. Statistical processing was carried out using the StatSoft program (Russia). The significance of the difference between the compared groups was determined using the Student's t test. Research results. An increase in indices of peripheral vascular resistance of the uterine arteries and a decrease in the systolic-diastolic ratio in the umbilical arteries in pregnant women with thyroid diseases were established, while more pronounced changes were observed in patients with autoimmune thyroiditis. Ultrasound data indicated the formation of fetoplacental insufficiency during pregnancy in women with thyroid diseases. Conclusion. To predict placental dysfunction and the formation of a risk group for the formation of perinatal complications, it is necessary to take into account the identified sonographic changes that are observed during pregnancy in patients with thyroid diseases.
The impact of cesarean section and its complications on fertility rates
Authors: Vlasova N. A. , Dikareva L. V. , Suverneva A. A. , Tishkova O. G. , Shvarev E. G.
Published: May 2024

The aim: to evaluate the relationship between performed caesarean section operations and complications influencing on fertility rates. Materials and methods. A total of 393 patients aged 19 to 43 years old have been examined to conduct aretrospective cohortre search. Some of their social and biological data, obstetric-gynecological anamnesis peculiarities (including extragenital diseases, development and complications of previous pregnancies), results of clinical, instrumental and laboratory examinations have been studied. Authenticity of difference between two independent groups in terms of qualitative characteristics was determined using Pearson's chi- square test (χ 2 ) (with Yates correction for small samples-df) by compiling four-field contingency tables. When performing analytical statistics, the following levels of significance of differences were established: p < 0,05; p < 0,01; p < 0,001. The statistical significance of the established relationship was being determined using the definition of relative risk (RR, relative risk) and 95 % confidence interval (CI, confidence interval). Results. After performed caesarean sections in the examined women non-developing pregnancy was being observed 1.9 times more often than in the comparison group in patients who gave birth through the natural birth canal (p = 0,003, RR = 1,886; CI = 1,061–3,351). Inflammatory diseases of the genitals in the main group (PID) were registered 1.7 times more often than in the comparison group (p < 0,001, RR = 1,667; CI = 1,290–2,156). It should be especially noted that after the completion of the first pregnancy by CS, repeated similar interventions were performed 6,5 times more often (p < 0,001, RR = 6,466; CI = 4,968–8,416). Other surgical interventions on the abdominal organs were performed 2,7 times more often than in the comparison group (p = 0,003, RR = 2,669; CI = 1,402–5,080). No less significant is the fact that proliferative diseases of the internal organs such as uterine myoma and endometriosis were being developed respectively 2 (χ 2 = 4,454, p = 0,035, RR = 2,062; CI = 1,052–4,508) and 4 times more often (χ 2=4,598, p = 0,033, RR = 4,311; CI = 1,281–14,508) than in patients of the comparison group. Conclusion. The completion of the reproductive debut by abdominal delivery is associated with a significant risk of developing PID, non-developing pregnancy, proliferative diseases of the uterus (myoma, endometriosis), necessity in performing repeated CS operations and other surgical interventions on abdominal organs.
Cinderela: Implicações da função materna nos processos de maturação do Ego
Authors: Silva Grabowski Bruna , Furtado Conte Raquel
Published: May 2024

A mãe suficientemente boa refere-se àquela figura capaz de interpretar e adaptar-se às necessidades do bebê, amparando-o fisiologicamente e afetivamente, o que oportuniza o estabelecimento de um ambiente favorável para os processos de maturação do Ego. Os contos de fadas são narrativas universais que contribuem na elaboração de conflitos internos, de modo a conectar as histórias à sua própria experiência. Nesse contexto, o objetivo deste estudo é identificar as possíveis implicações da função materna nos processos de maturação do Ego, a partir do conto de Fadas Cinderela. A presente pesquisa é de natureza qualitativa, com caráter exploratório e explicativo, cuja técnica para análise dos achados é a análise de conteúdo. Os resultados mostram que o papel da figura materna é crucial para que o sujeito seja capaz de tolerar a frustração; para o alcance do mundo simbólico; para adquirir independência, integração do Ego e desenvolvimento do self verdadeiro. Em consonância, os achados demonstram que os contos de fadas se configuram como um possível recurso de projeção da criança, bem como de sua figura materna, o que oportuniza elaboração de conflitos. Por meio deste trabalho, identificam-se as contribuições do uso de conto de fadas e sua influência no desenvolvimento emocional das crianças. Nesse sentido, a utilização deste trabalho pode beneficiar em âmbito social, as áreas científicas, em específico, a psicologia e a educação.
Este estudo aborda a necessidade de repensar a educação diante das transformações sociais, tecnológicas e econômicas, visando priorizar o desenvolvimento humano e a dignidade individual. Destacam-se as reflexões de Foucault sobre governamentalidade e responsabilização individual, juntamente com as análises de Benjamin e Freire sobre desumanização no contexto capitalista e a importância da educação humanista. Busca-se compreender como as abordagens pedagógicas, incluindo a Educação a Distância e sua aplicação diante de uma lógica de acesso a formação e seu uso dentro de uma lógica capitalista, podendo influenciar a formação humana
Authors: Chagas de Arruda Uedson
Published: May 2024

Diante da crescente utilização de tecnologias baseadas em inteligência artificial (IA), este estudo investiga como os alunos de Educação a Distância (EAD) do ensino superior estão incorporando a IA em sua rotina acadêmica e quais as contribuições dessa utilização em sua aprendizagem e no desempenho acadêmico. Os objetivos da pesquisa são: identificar as principais aplicações da IA utilizadas pelos alunos de EAD e verificar as contribuições da utilização da IA na aprendizagem e no desempenho acadêmico dos alunos de EAD. Para alcançar esses objetivos, foi adotada como metodologia a pesquisa qualitativa de estudo de caso, utilizando entrevistas semiestruturadas como instrumento de coleta de dados. O estudo foi realizado com alunos dos cursos de Pedagogia e administração em um polo de uma instituição de ensino superior (IES) privada de Recife-PE. De modo geral, os resultados expressam que a integração eficaz da IA no ambiente educacional pode transformar positivamente a forma como os alunos aprendem e se desenvolvem, preparando-os de forma mais eficiente para os desafios futuros em seu âmbito profissiona
Improving crop productivity in low land through crop intensification in Bangladesh
Authors: MAK Mian , AA Begum
Published: May 2024

Two experiments were conducted at Chalan Beel (Experiment-1: Sirajgonj) and at Monglar Beel (Experiment-2: Faridpur) to improve the rice-based cropping system in the lowlands during 2021 - 2022. Treatments of Experiment 1 were four cropping patterns, T1 = Mustard-Boro rice, T2 = Mustard-Jute, T3 = Potato-Boro rice, and T4 = Fallow-Boro rice. Treatments of Experiment 2 were four cropping patterns viz. T1 = Onion-Jute, T2 = Onion-Broadcast aman (B. aman) rice, T3 = Lathyrus- B. aman rice and T4 = Fallow-Boro rice. Experiments were conducted in an RCB design with five dispersed replications. Crops in the patterns were sown/planted/transplanted timely in the growing season. Cultural management was done as and when necessary as per the recommendation of each crop. All relevant data was collected and analyzed systematically. The results of Experiment 1 revealed that Mustard-Jute and Potato-Boro rice cropping patterns were found to be superior in respect of productivity and economic returns as compared to existing cropping patterns (ie. Fallow-Boro rice cropping pattern). The findings of Experiment 2 indicated that Onion-Jute and Onion-B. aman rice were found as the most productive cropping pattern with better economic benefits over existing cropping patterns (i.e. Fallow-Boro rice cropping pattern). Farmers are interested to adopt in these improved cropping patterns in the beel area. They will get more economic returns. The total food production of the country will be enhanced through the adoption of these improved cropping patterns.
HTTM : Transposon mutagenesis v2
Authors: Champie Antoine , De Grandmaison Amélie , Rodrigue Sebastien
Published: May 2024

Part of the HTTM protocol dedicated to the transposon mutagenesis of targets cells. Thelast stepin this version contains a supplemental video with extra context and tips, as part of the protocols.io Spotlight series, featuring conversations with protocol authors.
Sucrose Preference v1
Authors: daniel daniel.dautan , Svenningsson Per
Published: May 2024

Test for anhedonia-like behavior in mice.
Traces referential on raw material from Foz do Chapecó (Brazil) v1
Authors: Igier Lou , Tamara Jakab Lilla , SHRESTHA Vikram
Published: May 2024

Quite a few lithic use-wear referentials on raw materials from Brazil exist. To further develop use-wear analysis in the country and especially in the region of Foz do Chapecó, new referentials must be created to better understand trace formation on these specific raw materials. This protocol aims to use pieces in a sequential experiment to observe the initial development of wear traces. Given the short duration of the experiment, we do not expect to fully characterize the worked materials. Instead, our goal is to have a better understanding of the use-wear aspect on these raw materials, enabling us to identify relevant traces on archaeological artifacts.
Modelling Insulin resistance in vitro v1
Authors: Mahoney-Sanchez Laura
Published: May 2024

This protocol contains the instruction for modelling insulin resistance in iPSC-derived neurons by depriving the cells of insulin overnight followed by a 30 minutes insulin treatment the following day.
Планирование и управление туристическим кластером и отдельными туристическими объектами
Authors: Яковенко Д.А. , Лыков П.А. , Лыков В.А. , Бурданова Н.В.
Published: May 2024

В учебном пособии авторами рассматриваются вопросы управления как туристическим кластером, так и отдельными туристическими объектами. Проблема кластерного подхода, как эффективного инструмента долгосрочного развития туризма обсуждается достаточно давно в разных странах. По мнению авторов - туристский кластер - это совокупность туристско-рекреационных особых экономических зон, созданных при поддержке Правительства, расположенных на одной территории, являющихся определяющими в экономике данной территории. Цель создания кластера – создание мультипликационного синергетического эффекта в развитии экономики. Развитие туристского кластера неизбежно влияет на развитие всей инфраструктуры: транспорта, дорожного строительства и строительного комплекса в целом, на развитие сельского хозяйства и пищевой промышленности; существенно влияет на решение проблемы занятости населения. Кроме этого, туристские кластеры – это проводники создания положительной деловой репутации для страны, что может быть двигателем привлечения новых инвестиций. Наполнение кластера конкурентными туристическими объектами, относящимися к индустрии гостеприимства (гостиницы, кафе и рестораны) является необходимым условием успешного развития кластера. Учебно-методическое пособие для препода¬вателей и студентов профильных учеб¬ных заведений, готовящих профес¬сиональные кадры для предприятий ин¬дустрии гостеприимства, ставит своей целью удовлетворить реальные потреб¬ности специалистов индустрии гостеп-риимства в сис¬темных и практических знаниях, а также способствовать формированию конкурентоспособного кадрового потенциала. Работа может быть использована в качестве учебного пособия при обучении студентов по программам подготовки магистров по специальностям Государственное и муниципальное управление, Менеджмент.
PORTFOLIO OF RESEARCH PROJECTS AS A PREROGATIVE OF UNIVERSITIES TO ENSURE FINANCIAL AUTONOMY
Authors: Bohashko Oleksandr , Bohashko Iryna
Published: May 2024

The growing role of higher education in modern society and the expansion of the boundaries of science require a change in the models of university funding through the integration of certain corporate governance mechanisms. Of particular importance is the ability of universities to attract funding, including from private sources, by combining basic and applied research. It is critical for universities to find an optimal balance between basic and applied research when making decisions on launching new projects and programmes or adjusting existing ones. The purpose of the article is to determine the peculiarities of forming a balanced portfolio of research projects to ensure the financial autonomy of modern universities. The scientific research uses general scientific and special methods, in particular: analysis and synthesis; comparison, systematisation and generalisation, and the graphical method to clearly present the theoretical and practical provisions of the scientific article. The proposed approaches to assessing the university's portfolio of technological innovation developments will contribute to the efficient use of limited resources, prioritisation, risk analysis and monitoring of results in the short and long term. This will create a network of partners to share knowledge, research and resources.
MASTER'S DEGREE IN LAW - PER ASPERA AD ASTRA BALTIC APPROACH
Authors: Šulmane Dace , Beinoravičius Darijus , Kaugia Silvia
Published: May 2024

The purpose of this article is to continue the discussion of experts and academic representatives on the necessary knowledge and examination formats in law studies in order to achieve the goal: providing the education of highly qualified lawyers to fully and responsibly implement the rule of law in democratic countries. Authors highlight the current trends and novelties in the Baltic States, which illustrate the transformation of the attitude of legislators and policymakers and the steps taken to ensure the highest standards in learning the legal profession. The article illustrates the differences in the procedure for obtaining a master's degree in Latvia, Lithuania and Estonia, which is related to the introduction of the national unified legal professional qualification exam in Latvia in 2021. The article summarizes the opinions of three academic staff and legal professionals from the Baltic States on how the master's degree acquisition process existing in the country provides guarantees for graduates' acquisition of quality education and the necessary professional skills.
“SCHOOLS FOR LADIES AND GENTLEMEN” AS A SPECIFIC FORM OF NON-FORMAL EDUCATIONAL INSTITUTIONS IN FAR EAST COUNTRIES (China, Japan, South Korea and Singapore)
Authors: Kalashnyk Lyubov , Ruda Nataliia , Oserska Oksana , Nazarenko Oleksandra
Published: May 2024

”Schools for Ladies and Gentlemen" is a term commonly used to determine a non-formal educational institution in some of Far East countries where the students are taught mainly abroad ethics and aesthetics. Such educational institutions can be considered a vestige of the foreign countries` presence on the territory of the countries under consideration in the 19th-20th century, since it was the social activity of some European countries and US that largely determined the shape and value orientation of the education system in China, Japan, South Korea and Singapore in particular. The second half of the 20th century in the countries considered is characterized by a revision of foreign educational trends, which led to the departure of the abroad educational experience from the mass formal education. However, the social prestige of “foreign education” and the introduction of a child to “foreign ethical and aesthetic canons” as an indicator of financial well-being and social elitism has been preserved and has found its expression in the sphere of non-formal education in the form of clubs, circles, the services of private tutors, etc. The article examines the exact forms in which this trend is present in the educational sphere of one or another country in the Far East.
PEDAGOGICAL CONDITIONS FOR THE FORMATION OF READING CULTURE OF EDUCATION SEEKERS IN UKRAINIAN AND GLOBAL PRACTICE
Authors: Bilavych Halyna , Bagriy Maria , Klubochkina Tetiana , Pantyuk Mykola , Savchuk Borys
Published: May 2024

The aim of the article is to consider the formation of reading culture as an important pedagogical problem of today in the context of domestic and global problems of the development of students’ reading literacy, to single out the pedagogical conditions for the formation of the reading culture of an individual. Ukrainian and foreign scientists, teachers, and common people emphasize the crisis of children's reading: children and youth do not have a developed interest in this field of study, there is no motivation to read. This is a global trend. Pedagogical conditions for the formation of the reading culture of an individual are highlighted: from the teacher's point of view, this is high-quality preparation for teaching schoolchildren to read, mastery of a high level of reading culture, reading practice, children's reading circle; students believe that this is the activation of independent reading activity aimed at the development of abilities, interest in literature, improvement of knowledge, reading abilities and skills, active reading activity; parents think that it is the possession of reading culture, interest in reading, desire to ensure that fiction books do not lose their educational role among children, so that the great spiritual potential of fiction does not remain outside their attention, etc.
STUDY OF THE MODERN PARADIGM OF EDUCATIONAL DIPLOMACY: THE CASE OF UKRAINE
Authors: Mietule Iveta , Hushko Serhii , Maksymova Irina , Purii Hanna , Kulishov Volodymyr
Published: May 2024

The article examines the modern paradigm of education diplomacy through the elements of public diplomacy instead of classical understanding of diplomacy, namely, the formation of the image of an educational institution, interpersonal relations of participants in the educational process and the definition of new content in the global educational space. Besides, a discourse analysis is conducted, the main tasks of education diplomacy are formulated and defined. The methodological foundations of education diplomacy functioning are considered basing on generalizations of the experience and observations covering this issue, which indicate the multi-vector nature and relevance of the proposed research. Ukraine takes an important place in the development of global education diplomacy, due to positioning itself as a country of cultural and educational values on the international arena. The expert opinions of scientists studying the issue of education diplomacy from the point of view of current development, advantages and challenges are given in the article.  An integral part of education is scientific diplomacy, which has been gaining special relevance, since the publication and content of educational and scientific literature determine not only education in general, but also the educational and informational context of the country, in particular. Emphasizing positive trends and achievements in the field of education diplomacy, the authors, on the other hand, listed some problems and challenges in Ukraine, proposed relevant conclusions regarding the in-depth study of the issue. The result of the research was the proposed cluster model of educational diplomacy.
BURNOUT SYNDROME IN SLOVAK TEACHERS IN RELATION TO SELECTED VARIABLES OF THE JOB DEMAND RESOURCES MODEL
Authors: Markovič Daniel , Fričová Júlia , Kohútová Katarína
Published: May 2024

The aim of this paper is to explore and analyse the prevalence of risk factors (and factors) associated with burnout among Slovak teachers. The research sample consists of 274 respondents: teachers, school principals, and other pedagogical staff. We used the Job Demand Resources Model (JD-R model), which suggests that the interplay between job demands and resources determines the overall impact on employee well-being. Jobs with high demands and low resources are likely to lead to burnout and health issues, while jobs with high demands but sufficient resources can lead to positive outcomes such as increased motivation and job satisfaction. Investigating burnout in teachers is essential for promoting the well-being of educators, maintaining a high-quality education system, and ensuring positive outcomes for both teachers and learners. Based on the Job Demand Resources Model, this study aims to analyse the relationship between selected variables and burnout syndrome among Slovak teachers.
EXERCISE PROMOTION AMONG SCHOOL STUDENTS THROUGH STREET WORKOUT
Authors: Yaroshyk Marta , Bodnar Ivanna , Malanchuk Halyna , Rymar Olga , Solovei Alla , Semenova Nataliia , Stefanyshyn Orest , Stefanyshyn Nataliia , Petryna Roman
Published: May 2024

Today in Ukraine, there is a steady trend towards health deterioration of the younger generation. This negative phenomenon is associated with insufficient motor activity of young people (Rymar, Sorokolit, Solovey, Yaroshyk & Khanikiants, 2021; Solovey, Vovkanych, Sorokolit, Rymar, Yaroshyk & Novokshonov, 2021; Yaroshyk, Rymar, Malanchuk, Solovey, Khanikiants & Bubela, 2021). This problem can be solved by developing a positive attitude towards physical exercises, promoting trendy sports. Street workout is popular among young people. This type of activity is safe and suitable for anyone regardless of age, gender and level of physical fitness (Sanchez-Martinez, Plaza, Araneda, Sánchez & Almagiâ, 2017; Taipe-Nasimba &Cantón Chirivella, 2020). Purpose of the article – development of measures to promote physical activity among school students through Street workout. Methods: theoretical analysis and generalization; pedagogical observation; survey; mathematical statistics methods. A redistribution of self-assessed motor activity was recorded among school students at the end of the pedagogical observation after the implementation of the developed measures. Thus, the number of school students whose self-assessment grades were "excellent," "good," and "satisfactory" increased by 19.5%, 10% and 13.4% respectively. There was a 20.2% increase in the number of school students who regularly attended sports clubs. The number of school students who began to engage in physical activity at home increased by 12.8%. The number of school students who started doing morning gymnastics also increased by 13.5%. At the end of the pedagogical observation, the duration of sleep in most school students increased to 7-7.5 hours, and sleep quality improved and reached 6 points.
EARLY SCHOOL LEAVING RISKS RELATED TO EDUCATIONAL INSTITUTION
Authors: Kaniņa Inguna
Published: May 2024

Some of the main challenges in contemporary education systems are decrease of students learning motivation, students, who don’t engage in academic work and early school leaving. In Latvia the percentage of early school leavers still remains high. Main factors reported as the reason for early school leaving were not only risks related to students personality traits, family or social environment, but also risks related to school environment, diverging comprehension of the problems related to at-risk youth among teachers and school staff and different approaches within each school to prevent early school leaving. The method used in the research was questionnaires (with closed and open questions). 195 teachers from comprehensive schools (all districts) in Latvia were involved. The main goal of the research is to analyze  teachers comprehension and assessment on early school leaving risks related to school environment and to analyze teachers views about possibilities to identify at-risk youth at an early stage. Another goal of the research is to create a sustainable action plan, to reduce early school leaving risks related to school environment
TECHNOLOGIES FOR DEVELOPING THE CREATIVE POTENTIAL OF FUTURE VOCATIONAL TRAINING TEACHERS
Authors: Kovalchuk Vasyl , Vovk Lyudmyla , Volkova Natalііa , Vovk Bohdan , Horbatiuk Roman
Published: May 2024

The article examines the important aspect of training future teachers of professional education, namely applying technologies to stimulate and develop their creative potential. The formation and development of the creative potential of an individual is of great importance in the context of the educational process and the formation of the future teacher, since modern society has set high demands on specialists regarding their professional qualities. Applying the technologies for developing student’s creativity is a key element of the modern educational process aimed at stimulating creative thinking and personal development of the future specialist. The article explores current challenges associated with preparing future professional education educators for the implementation of educational technologies that stimulate the unfolding of learners' creative potential. The materials of this study focus on the issue of technologizing the educational process in higher education institutions, which will contribute to its qualitative improvement, wider adoption of innovative approaches, modern development tools, teaching methods, and forms that will ensure the development of learners' creative abilities. The concept of pedagogical technology is examined, its essence and classification are revealed, and aspects of implementing a technological approach in the educational process of preparing future professional education educators are highlighted. The results of the experimental research presented in the article emphasize the importance of using modern pedagogical technologies and draw attention to the need to search for rational ways of teaching, develop modern principles, and justify the specifics of their implementation that meet the requirements for education aimed at shaping and developing the creative personality of the future professional. The article highlights the practical experience of implementing educational technologies in the institutions of higher education and defines the effective methods of evaluating and supporting the creative potential of students. The key aspects that contribute to the improvement of the professional development of future specialists and the creation of conditions for their creative self-expression in the educational process and the further professional activities were considered.