bluuebunny/crossref_metadata_2025 huggingface dataset:
Muscle Carnitine Palmitoyltransferase II (CPT II) Deficiency: A Conceptual Approach
Authors: Joshi Pushpa Raj , Zierz Stephan
Published: April 2020

Carnitine palmitoyltransferase (CPT) catalyzes the transfer of long- and medium-chain fatty acids from cytoplasm into mitochondria, where oxidation of fatty acids takes place. Deficiency of CPT enzyme is associated with rare diseases of fatty acid metabolism. CPT is present in two subforms: CPT I at the outer mitochondrial membrane and carnitine palmitoyltransferase II (CPT II) inside the mitochondria. Deficiency of CPT II results in the most common inherited disorder of long-chain fatty acid oxidation affecting skeletal muscle. There is a lethal neonatal form, a severe infantile hepato-cardio-muscular form, and a rather mild myopathic form characterized by exercise-induced myalgia, weakness, and myoglobinuria. Total CPT activity (CPT I + CPT II) in muscles of CPT II-deficient patients is generally normal. Nevertheless, in some patients, not detectable to reduced total activities are also reported. CPT II protein is also shown in normal concentration in patients with normal CPT enzymatic activity. However, residual CPT II shows abnormal inhibition sensitivity towards malonyl-CoA, Triton X-100 and fatty acid metabolites in patients. Genetic studies have identified a common p.Ser113Leu mutation in the muscle form along with around 100 different rare mutations. The biochemical consequences of these mutations have been controversial. Hypotheses include lack of enzymatically active protein, partial enzyme deficiency and abnormally regulated enzyme. The recombinant enzyme experiments that we recently conducted have shown that CPT II enzyme is extremely thermoliable and is abnormally inhibited by different emulsifiers and detergents such as malonyl-CoA, palmitoyl-CoA, palmitoylcarnitine, Tween 20 and Triton X-100. Here, we present a conceptual overview on CPT II deficiency based on our own findings and on results from other studies addressing clinical, biochemical, histological, immunohistological and genetic aspects, as well as recent advancements in diagnosis and therapeutic strategies in this disorder.
Association between Self-Reported Eating Rate, Energy Intake, and Cardiovascular Risk Factors in a Multi-Ethnic Asian Population
Authors: Teo Pey Sze , van Dam Rob M. , Whitton Clare , Tan Linda Wei Lin , Forde Ciarán G.
Published: April 2020

Eating faster is associated with greater body mass index (BMI), but less is known about the relationships between eating rate, energy intake, body composition, and cardio-metabolic risk factors in different Asian ethnic groups. Using data from the Singapore Multi-Ethnic Cohort (n = 7011; 21–75 y), we investigated associations between self-reported eating rate (SRER), with energy intake, body composition, blood pressure, and blood lipids. SRER and lifestyle was assessed using interviewer-administered questionnaires. Multivariable models were used to examine the associations of SRER with energy intake, body composition, blood pressure, and blood lipids after adjusting for covariates. General and abdominal overweight were defined as BMI ≥ 23 kg/m2 and waist circumference >90 cm (men) and > 80 cm (women), respectively. On average, faster eaters (vs. slower eaters) consumed 105 kcal/day more (p = 0.034), had ~5 kg higher body weight (p < 0.001), 1.3 kg/m2 higher BMI (p < 0.001), and 3.1 cm larger waist-circumference (p < 0.001). Faster eaters had significantly higher blood pressure, circulating triglycerides, and total-to-high-density lipoprotein cholesterol ratio than slower eaters. Faster eaters were twice as likely to develop general (multivariable-OR: 2.2; 95% CI, 1.8–2.6; p < 0.001), and abdominal (OR: 1.8; 95% CI, 1.5–2.2; p < 0.001) overweight than slower eaters. This association was observed across all subgroups by age, sex, and ethnicity. Findings suggest that SRER is a robust behavioral marker for increased risk of higher energy intake, obesity, and poor cardio-metabolic health, and a modifiable behavioral risk-factor for obesity prevention.
Antecedent Dietary Glutamine Supplementation Benefits Modulation of Liver Pyroptosis in Mice with Polymicrobial Sepsis
Authors: Pai Man-Hui , Wu Jin-Ming , Yang Po-Jen , Lee Po-Chu , Huang Chun-Chieh , Yeh Sung-Ling , Lin Ming-Tsan
Published: April 2020

The liver is the main organ responsible for bacterial and endotoxin clearance. Pyroptosis is a form of proinflammatory programmed cell death activated by caspase-1/11 and gasdermin D (GadD). Pyroptosis protects the host against bacterial infection; however, overactive pyroptosis can lead to organ injury. Glutamine (GLN) is a specific amino acid with anti-inflammatory and immunomodulatory properties. This study investigated the effects of GLN pretreatment on liver pyroptosis in a mouse model of polymicrobial sepsis. Mice were assigned to sham, sepsis control (Sepsis-C), and sepsis GLN (Sepsis-G) groups. The sham and Sepsis-C groups were fed the AIN-93G diet. The Sepsis-G group was provided with identical diet components except that part of the casein was replaced by GLN. After feeding the respective diets for 2 weeks, a cecal ligation and puncture (CLP) procedure was performed in the sepsis groups. An antibiotic was administered after CLP. Mice were sacrificed at either 24 or 72 h after CLP. The results showed that sepsis resulted in upregulated liver caspase-1/11 expression. Compared to the Sepsis-C group, the Sepsis-G group had higher liver caspase-11 and NLRP3 gene expressions at 24 h and lower active caspase-1/11 and cleaved GadD protein levels at 72 h after sepsis. Additionally, liver inflammatory cytokine gene expressions had decreased by 72 h post-CLP. The findings suggest that prophylactic administration of GLN initially upregulated liver pyroptosis to eradicate pathogens, yet the process of pyroptosis was suppressed in the late phase of sepsis. This may have beneficially attenuated liver inflammation and injury in an antibiotic-treated septic condition.
Comparative Kinetics of Acetyl- and Butyryl-Cholinesterase Inhibition by Green Tea Catechins|Relevance to the Symptomatic Treatment of Alzheimer’s Disease
Authors: Okello Edward J. , Mather Joshua
Published: April 2020

Alzheimer’s disease (AD) is characterised by the apoptosis of cholinergic neurons and the consequent attenuation of acetylcholine mediated neurotransmission, resulting in neurodegeneration. Acetyl-cholinesterase (AChE) and butyryl-cholinesterase (BuChE) are attractive therapeutic targets in the treatment of AD since inhibition of these enzymes can be used to restore synaptic concentrations of acetylcholine. Whilst inhibitors for these enzymes such as galantamine and rivastigmine have been approved for use, none are able to halt the progression of AD and are responsible for the production of troublesome side-effects. Efficacious cholinesterase inhibitors have been isolated from natural plant-based compounds with many demonstrating additional benefits beyond cholinesterase inhibition, such as antioxidation and anti-inflammation, which are key parts of AD pathology. In this study, five natural flavan-3-ol (catechin) compounds: ((-)-epicatechin (EC), catechin, (-)-epicatechin-3-gallate (ECG),) (-)-epigallocatechin (EGC), (-)-epigallocatechin-3-gallate (EGCG), isolated from green tea, were screened for their cholinesterase inhibitory activity using the Ellman assay. The kinetics of inhibition was determined using reciprocal Lineweaver-Burk plots. EGCG was the only compound found to produce statistically significant, competitive inhibition, of both AChE (p < 0.01) and BuChE (p < 0.01) with IC50 values of 0.0148 µmol/mL and 0.0251 µmol/mL respectively. These results, combined with previously identified antioxidative and anti-inflammatory properties, highlight the potential use of EGCG in the treatment of AD, provided it can be delivered to cholinergic neurons in therapeutic concentrations. Further testing of EGCG in vivo is recommended to fully characterise the pharmacokinetic properties, optimal method of administration and efficacy of this novel plant-based compound.
Genotyping Echinococcus multilocularis in Human Alveolar Echinococcosis Patients: An EmsB Microsatellite Analysis
Authors: Knapp Jenny , Gottstein Bruno , Bretagne Stéphane , Bart Jean-Mathieu , Umhang Gérald , Richou Carine , Bresson-Hadni Solange , Millon Laurence
Published: April 2020

For clinical epidemiology specialists, connecting the genetic diversity of Echinococcus multilocularis to sources of infection or particular sites has become somewhat of a holy grail. It is very difficult to trace the infection history of alveolar echinococcosis (AE) patients as there may be an incubation period of five to 15 years before reliable diagnosis. Moreover, the variability of parasitic manifestations in human patients raises the possibility of genetically different isolates of E. multilocularis having different levels of pathogenicity. Thus, the exposure of human patients to different strains or genotypes circulating in geographically different environments may lead to different disease outcomes. Molecular tools, such as the microsatellite marker EmsB, were required to investigate these aspects. This genetic marker was previously tested on a collection of 1211 European field samples predominantly of animal origin, referenced on a publicly available database. In this study, we investigated a panel of 66 metacestode samples (between 1981 and 2019) recovered surgically from 63 patients diagnosed with alveolar echinococcosis originating from four European countries (France, Switzerland, Germany, Belgium). In this study, we identified nine EmsB profiles, five of which were found in patients located in the same areas of France and Switzerland. One profile was detected on both sides of the French-Swiss border, whereas most patients from non-endemic regions clustered together in another profile. EmsB profiles appeared to remain stable over time because similar profiles were detected in patients who underwent surgery recently and patients who underwent surgery some time ago. This study sheds light on possible pathways of contamination in humans, including proximity contamination in some cases, and the dominant contamination profiles in Europe, particularly for extrahepatic lesions.
Personalised 3D Printed Medicines: Optimising Material Properties for Successful Passive Diffusion Loading of Filaments for Fused Deposition Modelling of Solid Dosage Forms
Authors: Cerda Jose R. , Arifi Talaya , Ayyoubi Sejad , Knief Peter , Ballesteros Maria Paloma , Keeble William , Barbu Eugen , Healy Anne Marie , Lalatsa Aikaterini , Serrano Dolores R.
Published: April 2020

Although not readily accessible yet to many community and hospital pharmacists, fuse deposition modelling (FDM) is a 3D printing technique that can be used to create a 3D pharmaceutical dosage form by employing drug loaded filaments extruded via a nozzle, melted and deposited layer by layer. FDM requires printable filaments, which are commonly manufactured by hot melt extrusion, and identifying a suitable extrudable drug-excipient mixture can sometimes be challenging. We propose here the use of passive diffusion as an accessible loading method for filaments that can be printed using FDM technology to allow for the fabrication of oral personalised medicines in clinical settings. Utilising Hansen Solubility Parameters (HSP) and the concept of HSP distances (Ra) between drug, solvent, and filament, we have developed a facile pre-screening tool for the selection of the optimal combination that can provide a high drug loading (a high solvent-drug Ra, >10, and an intermediate solvent–filament Ra value, ~10). We have identified that other parameters such as surface roughness and stiffness also play a key role in enhancing passive diffusion of the drug into the filaments. A predictive model for drug loading was developed based on Support Vector Machine (SVM) regression and indicated a strong correlation between both Ra and filament stiffness and the diffusion capacity of a model BCS Class II drug, nifedipine (NFD), into the filaments. A drug loading, close to 3% w/w, was achieved. 3D printed tablets prepared using a PVA-derived filament (Hydrosupport, 3D Fuel) showed promising characteristics in terms of dissolution (with a sustained release over 24 h) and predicted chemical stability (>3 years at 25 °C/60% relative humidity), similar to commercially available NFD oral dosage forms. We believe FDM coupled with passive diffusion could be implemented easily in clinical settings for the manufacture of tailored personalised medicines, which can be stored over long periods of time (similar to industrially manufactured solid dosage forms).
miRNA Reference Genes in Extracellular Vesicles Released from Amniotic Membrane-Derived Mesenchymal Stromal Cells
Authors: Ragni Enrico , Perucca Orfei Carlotta , Silini Antonietta Rosa , Colombini Alessandra , Viganò Marco , Parolini Ornella , de Girolamo Laura
Published: April 2020

Human amniotic membrane and amniotic membrane-derived mesenchymal stromal cells (hAMSCs) have produced promising results in regenerative medicine, especially for the treatment of inflammatory-based diseases and for different injuries including those in the orthopedic field such as tendon disorders. hAMSCs have been proposed to exert their anti-inflammatory and healing potential via secreted factors, both free and conveyed within extracellular vesicles (EVs). In particular, EV miRNAs are considered privileged players due to their impact on target cells and tissues, and their future use as therapeutic molecules is being intensely investigated. In this view, EV-miRNA quantification in either research or future clinical products has emerged as a crucial paradigm, although, to date, largely unsolved due to lack of reliable reference genes (RGs). In this study, a panel of thirteen putative miRNA RGs (let-7a-5p, miR-16-5p, miR-22-5p, miR-23a-3p, miR-26a-5p, miR-29a-5p, miR-101-3p, miR-103a-3p, miR-221-3p, miR-423-5p, miR-425-5p, miR-660-5p and U6 snRNA) that were identified in different EV types was assessed in hAMSC-EVs. A validated experimental pipeline was followed, sifting the output of four largely accepted algorithms for RG prediction (geNorm, NormFinder, BestKeeper and ΔCt method). Out of nine RGs constitutively expressed across all EV isolates, miR-101-3p and miR-22-5p resulted in the most stable RGs, whereas miR-423-5p and U6 snRNA performed poorly. miR-22-5p was also previously reported to be a reliable RG in adipose-derived MSC-EVs, suggesting its suitability across samples isolated from different MSC types. Further, to shed light on the impact of incorrect RG choice, the level of five tendon-related miRNAs (miR-29a-3p, miR-135a-5p, miR-146a-5p, miR-337-3p, let-7d-5p) was compared among hAMSC-EVs isolates. The use of miR-423-5p and U6 snRNA did not allow a correct quantification of miRNA incorporation in EVs, leading to less accurate fingerprinting and, if used for potency prediction, misleading indication of the most appropriate clinical batch. These results emphasize the crucial importance of RG choice for EV-miRNAs in hAMSCs studies and contribute to the identification of reliable RGs such as miR-101-3p and miR-22-5p to be validated in other MSC-EVs related fields.
In Vitro Evaluation of Antimicrobial Activity of Minocycline Formulations for Topical Application in Periodontal Therapy
Authors: Schmid Jan-Luca , Kirchberg Martin , Sarembe Sandra , Kiesow Andreas , Sculean Anton , Mäder Karsten , Buchholz Mirko , Eick Sigrun
Published: April 2020

Periodontal therapy using antimicrobials that are topically applied requires slow or controlled release devices. The in vitro antimicrobial activity of biodegradable polymer formulations that contain a new minocycline lipid complex (P-MLC) was evaluated. The new P-MLC formulations that contained 11.5% minocycline were compared with pure minocycline or an existing commercial formulation, which included determination of minimal inhibitory concentration (MIC) values against two oral bacteria and activity on six-species periodontal biofilm. Moreover, the flow of gingival crevicular fluid (GCF) was modeled up to 42 days and the obtained eluates were tested both for MIC values and inhibiting biofilm formation. In general, MICs of the P-MLC formulations were slightly increased as compared with pure minocycline. Biofilm formation was clearly inhibited by all tested formulations containing minocycline with no clear difference between them. In 3.5 day old biofilms, all formulations with 250 µg/mL minocycline decreased bacterial counts by 3 log10 and metabolic activity with no difference to pure antimicrobials. Eluates of experimental formulations showed superiority in antimicrobial activity. Eluates of one experimental formulation (P503-MLC) still inhibited biofilm formation at 28 days, with a reduction by 1.87 log10 colony forming units (CFU) vs. the untreated control. The new experimental formulations can easily be instilled in periodontal pockets and represent alternatives in local antimicrobials, and thus warrant further testing.
Novel and Modified Neutrophil Elastase Inhibitor Loaded in Topical Formulations for Psoriasis Management
Authors: Nunes Andreia , Marto Joana , Gonçalves Lídia Maria , Simões Sandra , Félix Rita , Ascenso Andreia , Lopes Francisca , Ribeiro Helena Margarida
Published: April 2020

Human neutrophil elastase (HNE) is a serine protease that degrades matrix proteins. An excess of HNE may trigger several pathological conditions, such as psoriasis. In this work, we aimed to synthesize, characterize and formulate new HNE inhibitors with a 4-oxo-β-lactam scaffold with less toxicity, as well as therapeutic index in a psoriasis context. HNE inhibitors with 4-oxo-β-lactam scaffolds were synthesized and characterized by NMR, FTIR, melting point, mass spectrometry and elemental analysis. In vitro cytotoxicity and serine protease assays were performed. The compound with the highest cell viability (AAN-16) was selected to be incorporated in an emulsion (AAN-16 E) and in a microemulsion (AAN-16 ME). Formulations were characterized in terms of organoleptic properties, pH, rheology, droplet size distribution, in vitro drug release and in vivo psoriatic activity. All compounds were successfully synthesized according to analytical methodology, with good yields. Both formulations presented suitable physicochemical properties. AAN-16 E presented the most promising therapeutic effects in a murine model of psoriasis. Overall, new HNE inhibitors were synthesized with high and selective activity and incorporated into topical emulsions with potential to treat psoriasis.
AM-Induced Alteration in the Expression of Genes, Encoding Phosphorus Transporters and Enzymes of Carbohydrate Metabolism in Medicago lupulina
Authors: Yurkov Andrey , Kryukov Alexey , Gorbunova Anastasia , Sherbakov Andrey , Dobryakova Ksenia , Mikhaylova Yulia , Afonin Alexey , Shishova Maria
Published: April 2020

Plant–microbe interactions, including those of arbuscular mycorrhiza (AM), have been investigated for a wide spectrum of model plants. The present study focuses on an analysis of gene expression that encodes phosphate and sugar transporters and carbohydrate metabolic enzymes in a new model plant, the highly mycotrophic Medicago lupulina MLS-1 line under conditions of phosphorus deficiency and inoculation with Rhizophagus irregularis. Expression profiles were detected by RT-PCR at six plant stages of development (second leaf, third leaf, shooting, axillary shoot branching initiation, axillary shoot branching, flowering initiation). In comparison to control (without AM), the variant with AM inoculation exhibited a significant elevation of transcription levels of carbohydrate metabolic enzymes (MlSUS, MlHXK1) and sucrose transporters (MlSUC4) in M. lupulina leaves at the shooting stage. We suggest that this leads to a significant increase in the frequency of AM infection, an abundance of mycelium in roots and an increase in AM efficiency (which is calculated by the fresh weight of aerial parts and roots at the axillary shoot branching initiation stage). In roots, the specificity of MlPT4 and MlATP1 gene expressions were revealed for effective AM symbiosis. The level of MlPT4 transcripts in AM roots increased more than tenfold in comparison to that of non-specific MlPT1 and MlPT2. For the first time, MlPT1 expression was shown to increase sharply against MlPT2 in M. lupulina roots without AM at the shooting initiation stage. A significant increase in MlRUB expression was revealed at late stages in the host plant’s development, during axillary shoot branching and flowering initiation. The opposite changes characterized MlHXK1 expression. Alteration in MlHXK1 gene transcription was the same, but was more pronounced in roots. The obtained results indicate the importance of genes that encode phosphate transporters and the enzymes of carbohydrate metabolism for effective AM development at the shooting stage in the host plant.
Triacontanol Promotes the Fruit Development and Retards Fruit Senescence in Strawberry: A Transcriptome Analysis
Authors: Pang Qianqian , Chen Xueqin , Lv Jinhua , Li Teng , Fang Jinggui , Jia Haifeng
Published: April 2020

Triacontanol (TA) is a non-toxic, pollution-free, low-cost, high-efficiency, broad-spectrum plant growth regulator that plays an important role in plant growth and development, but its regulation mechanism of strawberry (Sweet charlie, Fragaria × ananassa Duch.) fruit development is still unclear. In this study, we showed that TA treatment (50 μM) could promote fruit development by up-regulating factors related to fruit ripening-related growth and development. TA increased fruit sugar content and anthocyanin accumulation, and many stress-related enzyme activities. In the meantime, Illumina RNA-Seq technology was used to evaluate the effect of TA treatment on strawberry fruit senescence. The results showed that 9338 differentially expressed genes (DEGs) were obtained, including 4520 up-regulated DEGs and 4818 down-regulated DEGs. We performed gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of these DEGs. The results showed that TA treatment caused changes in transcript levels related to cellular processes, hormones and secondary metabolism, such as DNA metabolic processes, flavonoid synthesis, and plant hormone signal transduction. Bioinformatics analysis showed that many transcription factors were related to fruit maturity. Taken together, this study will provide new insights into the mechanism of strawberry development and postharvest response to TA treatment.
Biochemical Analysis of Organic Acids and Soluble Sugars in Wild and Cultivated Pomegranate Germplasm Based in Pakistan
Authors: Nafees Muhammad , Jafar Jaskani Muhammad , Ahmad Ishtiaq , Maryam  , Ashraf Irfan , Maqsood Ambreen , Ahmar Sunny , Azam Muhammad , Hussain Sajjad , Hanif Asma , Chen Jen-Tsung
Published: April 2020

Pomegranate is famous for its health benefiting chemical and biochemical constituent compounds. The present study was undertaken to characterize pomegranate germplasm for its various fruit traits, acids, and sugar profiling through high performance liquid chromatography (HPLC) analysis. Among 11 detected acids and 8 sugars, citric acid and fructose were predominant in 18 domestic and 5 wild genotypes, respectively. Fruit weight, aril weight and wood portion index (WPI) were ranged from 15.82% to 24.42%, 10.99% to 113.78%, and 2.39% to 17.25%, respectively. Genotypes were grouped as sweet, sweet–sour, sour–sweet, and sour based on citric acid contents. Lactic acid and pyruvic acid showed the highest correlation (r = 0.92), however, sour and sweet genotypes had strong association for acids and sugars, respectively. Straddling of dendrogram showed the flow of genetic material in a cultivated location with wild and cultivated pomegranates grouped in different classes, however, wild and sour landraces grouped in the same class with 71% similarity of traits. Based on the observations of the current study, it was concluded that selected wild and arid zones (Multan, Bahawalpur) genotypes are poor in nutrients (acid and sugars) quality, however, genotypes of Rahim-Yar-Khan, Muzafar Garh, and Khyber Pakhtunkhwa have a better composition of sugars and acids.
Occurrence of Sweetpotato (Ipomoea batatas) Wilt and Surface Rot Disease and Determining Resistance of Selected Varieties to the Pathogen in Korea
Authors: Paul Narayan Chandra , Park Won , Lee Seungyong , Chung Mi Nam , Lee Hyeong-Un , Yang Jung-Wook
Published: April 2020

Fusarium wilt and Fusarium surface rot caused by Fusarium oxysporum Schltdl are the major diseases of sweetpotato (Ipomoea batatas) and was surveyed in different locations (Cheongju, Heanam, Iksan, Icheon, Kimje, Nonsan, Yeoungam, and Yeoju) in Korea from 2015 to 2017 in the field, after harvesting and in storehouse. The wilt incidence in the early stage represented 17.9%, 5.9%, and 8.3% in 2015, 2016, and 2017, respectively. Samples were collected, and the causal organism was isolated on potato dextrose agar (PDA). Ten pure cultures were stored at the Sweetpotato Research Laboratory, Bioenergy Crop Research Institute, Muan, Korea. Morphological analysis, along with molecular phylogeny of the sequences of internal-transcribed spacer (ITS) and elongation factor 1-α (EF-1α) genes and their combined phylogenetic analysis, confirmed the isolates as the Fusarium oxysporum Schltdl. Pathogenicity tests were conducted on sweetpotato stems, and storage roots by artificially inoculation methods, and the most virulent isolate was selected as SPL18019. A rapid screening method on 21 selected varieties for resistant variety selection was applied on stems. The Pungwanmi was found resistant to Fusarium wilt, whereas Annobeni was the most susceptible. On the other hand, six varieties were used to test surface rot resistance, and Yulmi and Yesumi were resistant and susceptible, respectively, to Fusarium surface rot.
Micropropagation and Production of Somatic Seeds for Short-Term Storage of the Endangered Species Eryngium alpinum L.
Authors: Kikowska Małgorzata , Sliwinska Elwira , Thiem Barbara
Published: April 2020

Eryngium alpinum L. is a high-value herb and a source of important compounds that include phenolics, triterpenoid saponins, and essential oils. The present report indicates successful micropropagation of this species. In our study, medium supplemented with BAP 2.0 mg/L, IAA 1.0 mg/L, and GA3 1.0 mg/L was found to be the most suitable for long-term culture and for effective proliferation, irrespective of the passage number. Roots induction, without basal callus formation, was observed when individual microshoots were placed on Murashige & Skoog medium augmented with auxin, and formation was the most advantageous in the presence of NAA alone or when combined with IAA or IBA. The encapsulated propagules were tested for their capability to endure different storage periods under low temperature. Therefore, we developed an efficient method for synseeds production by encapsulation of axillary buds in the sodium alginate matrix, storage for 2, 4, and 6 months, as well as the regeneration process. The maximum regeneration rate of 74% ± 2.72% was observed for axillary buds encapsulated in 4% sodium–alginate complexed with 300 mM calcium chloride after 2 months of storage at low temperature. This is the first report on E. alpinum micropropagation and somatic seeds production.
Supramolecular Dimerization in a Polymer Melt from Small-Angle X-ray Scattering and Rheology: A Miscible Model System
Authors: Staropoli Mariapaola , Kruteva Margarita , Allgaier Jürgen , Wischnewski Andreas , Pyckhout-Hintzen Wim
Published: April 2020

We present a structural and dynamic study on the simplest supramolecular hetero-association, recently investigated by the authors to prepare architectural homogeneous structures in the melt state, based on the bio-inspired hydrogen-bonding of thymine/diaminotriazine (thy–DAT) base-pairs. In the combination with an amorphous low Tg poly(butylene oxide) (PBO), no micellar structures are formed, which is expected for nonpolar polymers because of noncompatibility with the highly polar supramolecular groups. Instead, a clear polymer-like transient architecture is retrieved. This makes the heterocomplementary thy–DAT association an ideal candidate for further exploitation of the hydrogen-bonding ability in the bulk for self-healing purposes, damage management in rubbers or even the development of easily processable branched polymers with built-in plasticizer. In the present work, we investigate the temperature range from Tg + 20 °C to Tg + 150 °C of an oligomeric PBO using small-angle X-ray scattering (SAXS) and linear rheology on the pure thy and pure DAT monofunctionals and on an equimolar mixture of thy/DAT oligomers. The linear rheology performed at low temperature is found to correspond to fully closed-state dimeric configurations. At intermediate temperatures, SAXS probes the equilibrium between open and closed states of the thy–DAT mixtures. The temperature-dependent association constant in the full range between open and closed H-bonds and an enhancement of the monomeric friction coefficient due to the groups is obtained. The thy–DAT association in the melt is more stable than the DAT–DAT, whereas the thy–thy association seems to involve additional long-lived interactions.
Roles of Salicylate Donors in Enhancement of Productivity and Isotacticity of Ziegler–Natta Catalyzed Propylene Polymerization
Authors: Ratanasak Manussada , Hasegawa Jun-ya , Parasuk Vudhichai
Published: April 2020

Roles of internal salicylate donors (SID) in enhancing activity and stereoselectivity of Ziegler–Natta catalyzed propylene (PP) polymerization were examined using DFT calculations. Five salicylate donors were studied. The chelate mode is the preferred adsorption mode. The linear relationship (R2 = 0.96) between calculated adsorption energies (Eads) of five SIDs and the experimental PP activities was observed. Thus, the SID with the strongest adsorption energy will provide the highest activity in agreement with our previous studies. Compared with diisobutyl phthalate (DIBP), which is the industrial electron donor, SID has stronger Eads. The insertion step, which involves the π-complex formation (∆Eπ) and the insertion activation or intrinsic activation energy (Ea) for PP polymerization was also examined. The relation between ln(activity) and apparent activation energy (Ea(app)), which is ∆Eπ + Ea for the primary(1,2)-re insertion with R2 = 0.99, was observed. The salicylate donor also has a lower Ea(app) than that of DIBP. This explains the better catalytic performance of SID. Our results also demonstrated that the size and the type of hydrocarbon substituents play a key role in controlling stereoselectivity and activity. In addition, we found a good relationship between Eads and both intrinsic (Ea) and apparent (Ea(app)) activation energies of five salicylate donors with R2 of 0.90 and 0.97, respectively.
Poly(lactic Acid)–Biochar Biocomposites: Effect of Processing and Filler Content on Rheological, Thermal, and Mechanical Properties
Authors: Arrigo Rossella , Bartoli Mattia , Malucelli Giulio
Published: April 2020

Biocomposites based on poly(lactic acid) (PLA) and biochar (BC) particles derived from spent ground coffee were prepared using two different processing routes, namely melt mixing and solvent casting. The formulated biocomposites were characterized through rheological, thermal, and mechanical analyses, aiming at evaluating the effects of the filler content and of the processing method on their final properties. The rheological characterization demonstrated the effectiveness of both exploited strategies in achieving a good level of filler dispersion within the matrix, notwithstanding the occurrence of a remarkable decrease of the PLA molar mass during the processing at high temperature. Nevertheless, significant alterations of the PLA rheological behavior were observed in the composites obtained by melt mixing. Differential scanning calorimetry (DSC) measurements indicated a remarkable influence of the processing method on the thermal behavior of biocomposites. More specifically, melt mixing caused the appearance of two melting peaks, though the structure of the materials remained almost amorphous; conversely, a significant increase of the crystalline phase content was observed for solvent cast biocomposites containing low amounts of filler that acted as nucleating agents. Finally, thermogravimetric analyses suggested a catalytic effect of BC particles on the degradation of PLA; its biocomposites showed decreased thermal stability as compared with the neat PLA matrix.
Electroresponsive Polyelectrolyte Brushes Studied by Self-Consistent Field Theory
Authors: Okrugin Boris M. , Richter Ralf P. , Leermakers Frans A. M. , Neelov Igor M. , Zhulina Ekaterina B. , Borisov Oleg V.
Published: April 2020

End-grafting of polyelectrolyte chains to conducting substrates offers an opportunity to fabricate electro-responsive surfaces capable of changing their physical/chemical properties (adhesion, wettability) in response to applied electrical voltage. We use a self-consistent field numerical approach to compare the equilibrium properties of tethered strong and weak (pH-sensitive) polyelectrolytes to applied electrical field in both salt-free and salt-containing solutions. We demonstrate that both strong and weak polyelectrolyte brushes exhibit segregation of polyions in two populations if the surface is oppositely charged with respect to the brush. This segregation gives rise to complex patterns in the dependence of the brush thickness on salt concentration. We demonstrate that adjustable ionization of weak polyelectrolytes weakens their conformational response in terms of the dependence of brush thickness on the amplitude of the applied voltage.
Research on Inner Gas Inflation Improvements in Double-layer Gas-assisted Extrusion of Micro-tubes
Authors: Luo Cheng , Huang Xingyuan , Liu Tongke , Liu Hesheng
Published: April 2020

Micro-tubes have small diameters and thin wall thicknesses. When using double-layer gas-assisted extrusion (DGAE) technology to process micro-tubes, due to the influence of flow resistance, airflow from the inner gas-assisted layer cannot flow into the atmosphere through the lumen. Over time, it will inflate or even fracture the micro-tubes intermittently and periodically. To solve this problem, a new double-layer micro-tube gas-assisted extrusion die was designed in this study. Its mandrel has an independent airway leading to the lumen of the extrudate, with which the gas flow into the lumen of the extrudate can be regulated by employing forced exhaust. Using the new die, we carried out extrusion experiments and numerical calculations. The results show a significant positive correlation between micro-tube deformation and gas flow rate in the lumen of a micro-tube. Without considering the refrigerant distortion of the microtube, the flow rate of forced exhaust should be set equal to that of the gas from the inner gas-assisted layer flow into the micro-tube lumen. By doing this, the problem of the micro-tube being inflated can be eliminated without causing other problems.
Biopolymer-Inspired N-Doped Nanocarbon Using Carbonized Polydopamine: A High-Performance Electrocatalyst for Hydrogen-Evolution Reaction
Authors: Nguyen Duong Nguyen , Sim Uk , Kim Jung Kyu
Published: April 2020

Hydrogen-evolution reaction (HER) is a promising technology for renewable energy conversion and storage. Electrochemical HER can provide a cost-effective method for the clean production of hydrogen. In this study, a biomimetic eco-friendly approach to fabricate nitrogen-doped carbon nanosheets, exhibiting a high HER performance, and using a carbonized polydopamine (C-PDA), is described. As a biopolymer, polydopamine (PDA) exhibits high biocompatibility and can be easily obtained by an environmentally benign green synthesis with dopamine. Inspired by the polymerization of dopamine, we have devised the facile synthesis of nitrogen-doped nanocarbons using a carbonized polydopamine for the HER in acidic media. The N-doped nanocarbons exhibit excellent performance for H2 generation. The required overpotential at 5 mA/cm2 is 130 mV, and the Tafel slope is 45 mV/decade. Experimental characterizations confirm that the excellent performance of the N-doped nanocarbons can be attributed to the multisite nitrogen doping, while theoretical computations indicate the promotion effect of tertiary/aromatic nitrogen doping in enhancing the spin density of the doped samples and consequently in forming highly electroactive sites for HER applications.
How Much Lighting is Required to Feel Safe When Walking Through the Streets at Night?
Authors: Svechkina Alina , Trop Tamar , Portnov Boris A.
Published: April 2020

Public space lighting (PSL) is indispensable after the natural dark. However, little is known about how much PSL people actually need to feel sufficiently safe in different real-world urban settings. The present study attempts to answer this question by employing a novel real-time interactive approach, according to which, observers use a specially-designed mobile phone application to assess and report the perceived attributes of street lighting and the feeling of safety (FoS) it generates. To validate the proposed approach, a systematic survey was conducted in three cities in Israel—Tel Aviv-Yafo and Haifa, which lie on the Mediterranean coast, and Be’er Sheba, which lies inland. Additionally, instrumental PSL measurements were performed at the same locations. As the study reveals, the necessary level of illumination required by urban residents to feel safe differs by city and is significantly higher in Be’er Sheba, other factors held equal, in compare to Haifa and Tel Aviv-Yafo. This difference may be attributed to stronger daylight that the residents of the desert city of Be’er Sheba are accustomed to, and, therefore, may prefer stronger nighttime illumination. The difference could also be related to the relatively low socio-economic status and somewhat higher crime rates in the latter city. Findings also show a significant and positive association between FoS and instrumentally measured PSL levels, although this association exhibits diminishing returns. To the best of our knowledge, the present study is the first to use an interactive location- and time-based mobile phone technology, which can potentially provide more accurate and reliable assessments, compared to traditional “pen and paper” survey techniques.
Improving the Efficiency and Sustainability of Power Systems Using Distributed Power Factor Correction Methods
Authors: Coman Ciprian Mihai , Florescu Adriana , Oancea Constantin Daniel
Published: April 2020

For the equipment connected to the three-phase or single-phase grid, the power factor represents an efficiency measure for the usage of electrical energy. The power factor improvement through correction methods reduces the load on the transformers and power conductors, leading to a reduction of losses in the mains power supply and a sustainable grid system. The implications at the financial level are also important. An example of load that generates a small power factor is represented by a motor without mechanical load or having a small mechanical load. Given the power factor correction (PFC), the costs are reduced through the elimination of penalties, applying only in the common coupling point (CCP). The advantages of using equipment for the power factor correction are related also to their long operation duration and the easiness of their installation. The device presented in this article takes advantage of the advances in information and communication technology (ICT) to create a new approach for telemetry and remote configuration of a PFC. This approach has flexibility and versatility, such that it can be adapted to many loads, easily changing the capacitance steps and settings of the power factor correction device.
Gender Inequalities and Social Sustainability. Can Modernization Diminish the Gender Gap in Political Knowledge?
Authors: Roig Rosa , Aybar Cristina , Pavía Jose M.
Published: April 2020

The gender gap in political knowledge is a classical problem of Western democracies. In the 21st century, political knowledge is still unequally distributed between men and women, as many cross-section studies have shown. This is an indicator of women’s disempowerment and the distance which remains to be covered to achieve an inclusive and sustainable society. Could public policies and gender equality laws change the situation? Using a longitudinal database in which 600,000 survey responses are analysed from 1996 to 2017, this case study of Spain aims to shed some light on this question. It combines sociological and political approaches in line with the development theory of the gender gap of Inglehart and Norris (2000, 2003), whose core argument is that modernization changes cultural attitudes toward gender equality. From this perspective, this paper proposes the following hypothesis: the modernization process of Spain (from a dictatorship to a democracy) has given rise to changes in traditional sex roles, driving women‘s access to political knowledge and diminishing the gender gap. This is a step towards achieving objective number 5 of the 2030 United Nations Agenda for Sustainable Development (gender equality and empowerment of women and girls), according to which gender equality is not only a fundamental human right but a necessary foundation for a peaceful, prosperous and sustainable world.
A New Livelihood Sustainability Index for Rural Revitalization Assessment—A Modelling Study on Smart Tourism Specialization in China
Authors: Li Huiqin , Nijkamp Peter , Xie Xuelian , Liu Jingjing
Published: April 2020

In our rapidly urbanizing world, the problem of rurality versus urbanization is becoming a source of concern. Rural tourism development may become a new important stimulus for promoting a sustainable transformation of the farmers’ livelihood. This calls for a smart specialization in rural tourism where the focus is on a balanced rural revitalization strategy. As part of the empirical research, this paper introduces a livelihood sustainability index. This index helps to construct a balanced system for the evaluation of livelihood sustainability achievements in rural tourism destinations. It is based on livelihood capital, livelihood strategy, and the interlinkage between livelihood and environment, in order to dynamically assess the livelihood sustainability of rural households. Taking Huangpi District of the Wuhan area in China as our applied case study, the livelihood sustainability index appears to show over the past years a significant rise, based on a comprehensive index method, an entropy method, and a coupling coordination model. Our findings show that the development of rural tourism has clearly promoted livelihood sustainability. This has inter alia resulted in the accumulation of livelihood capital, an asset of which both social and cultural capital have benefitted greatly; livelihood strategies have also improved, and therefore so have livelihood diversity and stability; and finally, the interlinkage and coordination degree between livelihood and the environment has also changed positively from a primary to intermediate balanced development. However, the livelihood sustainability index in the area concerned is still relatively low, and has not yet reached its possible optimal level. Hence, there is still much room for improvement. Various approaches can be proposed to achieve a more sustainable livelihood, such as enhancing livelihood capital; narrowing the economic gap between farmers by participating in professional tourism activity; establishing the mechanism of industrial integration and the development of rural eco-tourism; and coordinating a balanced development of livelihood and environmental quality.
Public Acceptance of the “Renewable Energy 3020 Plan”: Evidence from a Contingent Valuation Study in South Korea
Authors: Kim Ju-Hee , Kim Sin-Young , Yoo Seung-Hoon
Published: April 2020

South Korea is promoting the “Renewable Energy 3020 Plan” to expand the proportion of renewable energy (RE) from 2.2% in 2016 to 20% in 2030. Since the plan could lead to an increase in electricity rates, public acceptance of it is an important key to determining its success. This article examines the public acceptance of the plan by employing contingent valuation (CV). A nationwide CV survey of 1000 households was performed to collect the data on the willingness to pay (WTP) for implementing the plan using electricity charges as a payment vehicle during October 2018. More specifically, a dichotomous choice question to ask interviewees whether they have an intention of paying an offered bid and a spike model to treat the zero WTP observations were employed. The estimate for monthly WTP was KRW 3646 (USD 3.27) per household. When 10 years of the payment period presented in the survey and forecasted values of residential RE electricity consumption from 2018 to 2030 were used, the WTP was worth KRW 60.4 (USD 0.05) per kWh in 2018 constant price. The WTP amounts to 56.5% of the price for residential electricity (KRW 106.9 or USD 0.10 per kWh). That is, the RE electricity produced through implementing the plan has a premium of 56.5% over the current electricity. Overall, it is concluded that public acceptance of the plan has been secured.
Comparison between Sexes of the Relationships between Body Composition and Maximum Oxygen Volume in Elderly People
Authors: Onetti-Onetti Wanesa , Molina-Sotomayor Edgardo , González-Jurado José Antonio , Castillo-Rodríguez Alfonso
Published: April 2020

Background: Performing physical activity can provide a variety of benefits; for example, it can improve one’s physical condition, decrease one’s fat mass, and increase one’s muscle mass. The objective of the present study was to evaluate the relationships between the cardiorespiratory resistance and physical characteristics of people over 60 years of age. Methods: A total of 31 participants over 60 years of age from the province of Malaga (Spain) joined the study voluntarily. Results: The aerobic capacity test, called the Rockport One Mile, was carried out, and Spearman correlation and linear regression analyses between the volume of oxygen (VO2) and the body weight, body mass index (BMI) and age of the participants were performed. The results indicated that 28% (in women) and 48% (in men) of the maximum VO2 (determined by the Rockport test) was explained by the variable body weight (p < 0.01). Conclusions: The main finding of this study is that an individual’s physical abilities, specifically resistance and aerobic capacity are directly related to his or her body weight and other variables in males, such as age and BMI.
Improving Residential Load Disaggregation for Sustainable Development of Energy via Principal Component Analysis
Authors: Moradzadeh Arash , Sadeghian Omid , Pourhossein Kazem , Mohammadi-Ivatloo Behnam , Anvari-Moghaddam Amjad
Published: April 2020

The useful planning and operation of the energy system requires a sustainability assessment of the system, in which the load model adopted is the most important factor in sustainability assessment. Having information about energy consumption patterns of the appliances allows consumers to manage their energy consumption efficiently. Non-intrusive load monitoring (NILM) is an effective tool to recognize power consumption patterns from the measured data in meters. In this paper, an unsupervised approach based on dimensionality reduction is applied to identify power consumption patterns of home electrical appliances. This approach can be utilized to classify household activities of daily life using data measured from home electrical smart meters. In the proposed method, the power consumption curves of the electrical appliances, as high-dimensional data, are mapped to a low-dimensional space by preserving the highest data variance via principal component analysis (PCA). In this paper, the reference energy disaggregation dataset (REDD) has been used to verify the proposed method. REDD is related to real-world measurements recorded at low-frequency. The presented results reveal the accuracy and efficiency of the proposed method in comparison to conventional procedures of NILM.