bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of Medication Errors Reported by Community Pharmacists in the Republic of Korea: A Cross-Sectional Study
Authors: Han Ju-Hee , Heo Kyu-Nam , Han JiMin , Lee Mo-Se , Kim Su-Jin , Min Sangil , Ah Young-Mi , Lee Ju-Yeun
Published: January 2023

Background and objectives: We aimed to describe medication-related incidents or medication errors (MEs) reported by community pharmacists and analyze the prevalent medications involved. Materials and Methods: We extracted ME reports from databases comprising patient safety incidents reported to the Korean Pharmaceutical Association between January 2013 and June 2021. Medications were analyzed according to the second (therapeutic subgroup) and fifth (chemical substance) levels of the Anatomical Therapeutic Chemical classification. Results: A total of 9046 MEs were identified, most of which were near miss reports (88.3%). Among the errors that reached the patients (521 cases), harmful incidents accounted for 76.8%. Most MEs occurred during prescription (89.5%), while harmful MEs occurred mainly during dispensing (73.3%). In the prescription step, wrong drugs (44.8%), dosing errors (27.0%), and wrong durations (14.0%) were common. Anti-inflammatory and anti-rheumatic products (M01), drugs for acid-related disorders (A02), and antihistamines for systemic use (R06) were the most frequently reported medication classes involved. Harmful incidents were most common for dosing errors (31.0%) and wrong drugs (26.8%) and were common with warfarin, levothyroxine, and glimepiride. Conclusions: The MEs reported by community pharmacists were mainly prescribing errors, most of which were rectified before reaching patients. The prevalent medications involved in harmful errors include anti-diabetic, anti-thrombotic, and anti-inflammatory agents.
Impact of a Targeted Project for Shortening of Imaging Diagnostic Waiting Time in Patients with Suspected Oncological Diseases in Hungary
Authors: Papp Zsombor Mátyás , Szakács László , Hajivandi Shayan-Salehi , Kalina Ildikó , Varga Edit , Kiss Gergely , Solymos Ferenc , Takács István , Dank Magdolna , Dudás Ibolyka , Szanka Tímea , Dózsa Csaba László , Rékassy Balázs , Merkely Béla , Maurovich-Horvat Pál
Published: January 2023

Background and Objectives: Medical imaging is a key element in the clinical workup of patients with suspected oncological disease. In Hungary, due to the high number of patients, waiting lists for Computed Tomography (CT) and Magnetic Resonance Imaging (MRI) were created some years ago. The Municipality of Budapest and Semmelweis University signed a cooperation agreement with an extra budget in 2020 (HBP: Healthy Budapest Program) to reduce the waiting lists for these patients. The aim of our study was to analyze the impact of the first experiences with the HBP. Material and Methods: The study database included all the CT/MRI examinations conducted at Semmelweis University with a referral diagnosis of suspected oncological disease within the first 13 months of the HBP (6804 cases). In our retrospective, two-armed, comparative clinical study, different components of the waiting times in the oncology diagnostics pathway were analyzed. Using propensity score matching, we compared the data of the HBP-funded patients (n = 450) to those of the patients with regular care provided by the National Health Insurance Fund (NHIF) (n = 450). Results: In the HBP-funded vs. the NHIF-funded patients, the time interval from the first suspicion of oncological disease to the request for imaging examinations was on average 15.2 days shorter (16.1 vs. 31.3 days), and the mean waiting time for the CT/MRI examination was reduced by 13.0 days (4.2 vs. 17.2 days, respectively). In addition, the imaging medical records were prepared on average 1.7 days faster for the HBP-funded patients than for the NHIF-funded patients (3.4 vs. 5.1 days, respectively). No further shortening of the different time intervals during the subsequent oncology diagnostic pathway (histological investigation and multidisciplinary team decision) or in the starting of specific oncological therapy (surgery, irradiation, and chemotherapy) was observed in the HBP-funded vs. the NHIF-funded patients. We identified a moderately strong negative correlation (r = −0.5736, p = 0.0350) between the CT/MR scans requested and the active COVID-19 case rates during the pandemic waves. Conclusion: The waiting lists for diagnostic CT/MR imaging can be effectively shortened with a targeted project, but a more comprehensive intervention is needed to shorten the time from the radiological diagnosis, through the decisions of the oncoteam, to the start of the oncological treatment.
Direct Membrane Filtration of Municipal Wastewater: Studying the Most Suitable Conditions for Minimizing Fouling Rate in Commercial Porous Membranes at Demonstration Scale
Authors: Sanchis-Perucho Pau , Aguado Daniel , Ferrer José , Seco Aurora , Robles Ángel
Published: January 2023

This study aimed to evaluate the feasibility of applying a commercial porous membrane to direct filtration of municipal wastewater. The effects of membrane pore size (MF and UF), treated influent (raw wastewater and the primary settler effluent of a municipal wastewater treatment plant) and operating solids concentration (about 1 and 2.6 g L−1) were evaluated on a demonstration plant. Filtration periods of 2–8 h were achieved when using the MF membrane, while these increased to 34–69 days with the UF membrane. This wide difference was due to severe fouling when operating the MF membrane, which was dramatically reduced by the UF membrane. Use of raw wastewater and higher solids concentration showed a significant benefit in the filtration performance when using the UF module. The physical fouling control strategies tested (air sparging and backwashing) proved to be ineffective in controlling UF membrane fouling, although these strategies had a significant impact on MF membrane fouling, extending the operating period from some hours to 5–6 days. The fouling evaluation showed that a cake layer seemed to be the predominant reversible fouling mechanism during each independent filtration cycle. However, as continuous filtration advanced, a large accumulation of irreversible fouling appeared, which could have been related to intermediate/complete pore blocking in the case of the MF membrane, while it could have been produced by standard pore blocking in the case of the UF membrane. Organic matter represented more than 70% of this irreversible fouling in all the experimental conditions evaluated.
Incorporating the Roles of Feature Size and Build Orientation in the Process-Structure-Property Relationship for Additively Manufactured Parts
Authors: Phutela Chinmay , Bosio Federico , Alhammadi Alya , Alshehhi Nujood , Kindleyside Lewis , Aboulkhair Nesma T.
Published: January 2023

Laser powder bed fusion (L-PBF) additive manufacturing has reached wide-scale technology readiness for various sectors. However, some challenges posed by the complex nature of the process persist. Limited studies investigated the correlation between the micro- and macroscopic properties of L-PBF AlSi10Mg parts and the features’ sizes with the build orientation in mind. Therefore, this study presents a comprehensive view on the “size effect” for samples larger than those available in the literature (up to 12 mm) on the defects, microstructure evolution, and mechanical properties in two build orientations using a fixed set of process parameters. Microstructural differences were observed between the build orientations, but no considerable difference with size change was detected. The porosity content was inversely proportional to the feature size irrespective of the build orientation, leading to an increase in ductility that was more evident in the horizontal specimens (~44%). This was attributed to an in-situ heat treatment. Although specimens oriented parallel to the build direction showed no significant size-effect in terms of the mechanical properties (hardness and tensile), anisotropy was evident. Based on the findings presented in the study and the scientific explanations discussed corroborated by thermal imaging during processing, it is concluded that although any set of ‘optimised’ process parameters will only be valid for a specific size range, the severity of the size-effect changes dynamically based on the range.
Effect of Toughness and Ductility on the Cavitation Erosion of Martensitic Stainless Steel
Authors: Zhao Lianqing , Hu Hongxiang , Guo Xuming
Published: January 2023

Martensitic stainless steel containing 13% Cr–4% Ni suffers cavitation erosion (CE) as the common material of hydro turbine impellers. Two 13% Cr–4% Ni stainless steel samples were obtained by different melting and heating processes. One was of relatively low toughness but high ductility (LTHD), and the other was of relatively high toughness but low ductility (HTLD). This paper is to clarify the relationship between the mechanical properties and the CE resistance of the experimental steel samples. The CE of the two materials was studied using an ultrasonic vibration cavitation erosion rig. Mass loss, morphological observation, nanoindentation characterization, and tensile tests were employed to clarify the erosion mechanism. The results showed that LTHD stainless steel had slightly higher ductility, but lower toughness than HTLD material. The mass loss method verified that the CE resistance of LTHD material was higher than that for the HTLD material. In addition, both materials had an incubation stage of 2 h in the distilled water. The SEM revealed that material removal was preferentially initiated from the grain boundaries and slip zone after the incubation period. The ductility could delay the fracture of the material, which contributed more to cavitation erosion resistance than the toughness of the materials. The hardness test showed few relationships with the CE resistance.
Mechanism of Layer Formation during Gas Nitriding of Remelted Ledeburitic Surface Layers on Unalloyed Cast Irons
Authors: Holst Anja , Kante Stefan , Leineweber Andreas , Buchwalder Anja
Published: January 2023

Unalloyed cast iron materials exhibit low tribological and corrosive resistance. In this respect, nitriding has a wide range of applications for steels. In the case of cast iron, the advantageous properties of nitrided layers are impaired by the presence of graphite. Electron beam remelting of cast iron surfaces prior to nitriding removes graphite. The homogeneous ledeburitic microstructure within the approx. 1 mm-thick remelted layer enables the formation of a dense compound layer during subsequent nitriding. The main objective of this study is to investigate the nitriding mechanism of unalloyed ledeburitic microstructures. Due to the complex relationships, investigations were carried out on both conventional ferritic and pearlitic cast irons and Fe-based model alloys containing one to four additional alloying elements, i.e., C, Si, Mn and Cu. The iron (carbo-)nitride composition (γ’, ε) of this compound layer depends on the gas nitriding conditions, the chemical composition of the substrates and the microstructural constituents. As a result, a schematic model of the nitriding mechanism is developed that includes the effects of the nitriding parameters and alloy composition on the phase composition of the nitriding layer. These findings enable targeted parameter selection and a further optimization of both the process and the properties.
Microstructure and High-Temperature Mechanical Properties of Mg-1Al-12Y Alloy Containing LPSO Phase
Authors: Ren Qianlong , Yuan Shuai , Wang Jinhui , Ma Deqing , Li Wei , Wang Lei
Published: January 2023

This paper describes our study on the effect of the long-period stacking order (LPSO) phase on the comprehensive mechanical properties of the as-cast Mg-1Al-12Y (wt. %) alloy. The microstructure and tensile mechanical properties of as-cast alloy and solution treatment alloy were evaluated. The results showed that α-Mg matrix, Al2Y phase and β-Mg24Y5 phase were present in the as-cast alloy. After solution treatment, the β-Mg24Y5 phase in the alloy was dissolved and the LPSO phase was precipitated. The solution treatment did not cause grain growth or Al2Y phase change, but the comprehensive mechanical properties of the alloy were significantly improved. This was mainly due to the precipitation of the LPSO phase in the solid solution alloy. In addition, the dissolution of the β-Mg24Y5 phase after solution treatment could also improve the mechanical properties. The improvement of the comprehensive mechanical properties of solution treatment alloys at room temperature was due to the dispersion strengthening caused by the intragranular LPSO phase dispersed within the alloy, which had a good blocking effect on the movement of dislocations. The ductility of the solid-solution alloy was greatly improved at a high temperature while maintaining high strength because the LPSO phase is kinked, the dislocation density was reduced, and more non-basal slip was activated during the high-temperature tension.
Seasonal Variations of Low-Molecular-Weight Organic Acids in Three Evergreen Broadleaf Rhododendron Forests
Authors: Lv Xiaofei , Hao Jiangtao , Zhao Yumeng , Li Chaochan , Quan Wenxuan
Published: January 2023

Low-molecular-weight organic acids (LMWOAs) are widely distributed in forests. Fresh leaves, litter, humus, and the topsoil layer of representative Rhododendron delavayi (RD), Rhododendron agastum (RA), and Rhododendron irroratum (RI) in the Baili Rhododendron National Forest Park were sampled to explore their seasonal changes. The contents of oxalic, tartaric, malic, citric, acetic, lactic, succinic, and formic acids in samples from different seasons were determined by high-performance liquid chromatography. The results showed that the composition and content of the LMWOAs in the fresh leaves, litter, humus, and topsoil layer of the rhododendrons were affected by the tree species, samples, and season. The main LMWOA was oxalic acid (the average content in the samples was 195.31 µg/g), followed by malic acid (the average content in the samples was 68.55 µg/g) and tartaric acid (the average content in the samples was 59.82 µg/g). Succinic acid had the lowest content; the average content in the samples was 18.40 µg/g. The LMWOAs of the RD were the highest (the average content in the samples was 517.42 µg/g), and the LMWOAs of the RI were the lowest (the average content in the samples was 445.18 µg/g). The LMWOAs in the three rhododendron forests were in the order of fresh leaves > litter > humus > soil layers. This study showed the seasonal distribution characteristics of LMWOAs in three evergreen broadleaf rhododendron forests, and the results provide a reference for ecosystem management and the protection of wild rhododendron forests.
Data-Driven Design of Transparent Thermal Insulating Nanoscale Layered Oxides
Authors: Wu Yen-Ju , Xu Yibin
Published: January 2023

Predicting the interfacial thermal resistance (ITR) for various material systems is a time-consuming process. In this study, we applied our previously proposed ITR machine learning models to discover the material systems that satisfy both high transparency and low thermal conductivity. The selected material system of TiO2/SiO2 shows a high ITR of 26.56 m2K/GW, which is in good agreement with the predicted value. The nanoscale layered TiO2/SiO2 thin films synthesized by sputtering exhibits ultralow thermal conductivity (0.21 W/mK) and high transparency (>90%, 380–800 nm). The reduction of the thermal conductivity is achieved by the high density of the interfaces with a high ITR rather than the change of the intrinsic thermal conductivity. The thermal conductivity of TiO2 is observed to be 1.56 W/mK with the film thickness in the range of 5–50 nm. Furthermore, the strong substrate dependence is confirmed as the thermal conductivity of the nanoscale layered TiO2/SiO2 thin films on quartz glass is three times lower than that on Si. The proposed TiO2/SiO2 composites have higher transparency and robustness, good adaptivity to electronics, and lower cost than the current transparent thermal insulating materials such as aerogels and polypropylene. The good agreement of the experimental ITR with the prediction and the low thermal conductivity of the layered thin films promise this strategy has great potential for accelerating the development of transparent thermal insulators.
GaN JBS Diode Device Performance Prediction Method Based on Neural Network
Authors: Ma Hao , Duan Xiaoling , Wang Shulong , Liu Shijie , Zhang Jincheng , Hao Yue
Published: January 2023

GaN JBS diodes exhibit excellent performance in power electronics. However, device performance is affected by multiple parameters of the P+ region, and the traditional TCAD simulation method is complex and time-consuming. In this study, we used a neural network machine learning method to predict the performance of a GaN JBS diode. First, 3018 groups of sample data composed of device structure and performance parameters were obtained using TCAD tools. The data were then input into the established neural network for training, which could quickly predict the device performance. The final prediction results show that the mean relative errors of the on-state resistance and reverse breakdown voltage are 0.048 and 0.028, respectively. The predicted value has an excellent fitting effect. This method can quickly design GaN JBS diodes with target performance and accelerate research on GaN JBS diode performance prediction.
ZnO/Graphene Composite from Solvent-Exfoliated Few-Layer Graphene Nanosheets for Photocatalytic Dye Degradation under Sunlight Irradiation
Authors: Venkidusamy Vasanthi , Nallusamy Sivanantham , Nammalvar Gopalakrishnan , Veerabahu Ramakrishnan , Thirumurugan Arun , Natarajan Chidhambaram , Dhanabalan Shanmuga Sundar , Pabba Durga Prasad , Abarzúa Carolina Venegas , Kamaraj Sathish-Kumar
Published: January 2023

ZnO/graphene nanocomposites were prepared using a facile approach. Graphene nanosheets were prepared by ultrasonication-based liquid phase exfoliation of graphite powder in a low boiling point organic solvent, 1,2-Dichloroethane, for the preparation of ZnO/graphene nanocomposites. Structural properties of the synthesized ZnO/graphene nanocomposites were studied through powder XRD and micro-Raman analysis. The characteristic Raman active modes of ZnO and graphene present in the micro-Raman spectra ensured the formation of ZnO/graphene nanocomposite and it is inferred that the graphene sheets in the composites were few layers in nature. Increasing the concentration of graphene influenced the surface morphology of the ZnO nanoparticles and a flower shape ZnO was formed on the graphene nanosheets of the composite with high graphene concentration. The efficiencies of the samples for the photocatalytic degradation of Methylene Blue dye under sunlight irradiation were investigated and 97% degradation efficiency was observed. The stability of the nanocomposites was evaluated by performing five cycles, and 92% degradation efficiency was maintained. The observed results were compared with that of ZnO/graphene composite derived from other methods.
A Study on High-Rate Performance of Graphite Nanostructures Produced by Ball Milling as Anode for Lithium-Ion Batteries
Authors: Ghanooni Ahmadabadi Vahide , Rahman Md Mokhlesur , Chen Ying
Published: January 2023

Graphite, with appealing features such as good stability, high electrical conductivity, and natural abundance, is still the main commercial anode material for lithium-ion batteries. The charge-discharge rate capability of graphite anodes is not significant for the development of mobile devices and electric vehicles. Therefore, the feasibility investigation of the rate capability enhancement of graphite by manipulating the structure is worthwhile and of interest. In this study, an effective ball-milling process has been set up by which graphite nanostructures with a high surface area are produced. An in-depth investigation into the effect of ball milling on graphite structure as well as electrochemical performance, particularly rate capability, is conducted. Here, we report that graphite nanoflakes with 350 m2 g−1 surface area deliver retained capacity of ~75 mAh g−1 at 10 C (1 C = 372 mA g−1). Finally, the Li+ surface-storage mechanism is recognised by associating the structural characteristics with electrochemical properties.
Epiphytic and Endophytic Fungi Colonizing Seeds of Two Poaceae Weed Species and Fusarium spp. Seed Degradation Potential In Vitro
Authors: Ņečajeva Jevgenija , Borodušķe Anete , Nikolajeva Vizma , Seņkovs Māris , Kalniņa Ineta , Roga Ance , Skinderskis Edmunds , Fridmanis Dāvids
Published: January 2023

Fungi colonizing the surface and endosphere of two widespread Poaceae weed species, Avena fatua and Echinochloa crus-galli, were isolated to compare the taxonomic composition between the plant species, location, and year of the seed collection. The seed-degrading potential of Fusarium isolated from the seeds was tested by inoculating seeds of E. crus-galli with spore suspension. Molecular identification of epiphytic and endophytic fungal genera was performed by sequencing the ITS region of rDNA. Endophytes comprised of significantly lower fungal richness compared to epiphytes. A significant taxonomic overlap was observed between the endosphere and seed surface. The most abundant genera were Alternaria, Fusarium, Cladosporium, and Sarocladium. Analysis of similarities and hierarchical clustering showed that microbial communities were more dissimilar between the two plant species than between the years. Fusarium isolates with a high potential to infect and degrade E. crus-galli seeds in laboratory conditions belong to F. sporotrichioides and F. culmorum.
Are ELISA and PCR Discrepancies in the Identification of Chlamydia pneumoniae Caused by the Presence of “Chlamydia-Related Bacteria”?
Authors: Smolejová Martina , Krčmáriková Jana , Cihová Iveta , Sulo Pavol
Published: January 2023

Chlamydia are Gram-negative, intracellular pathogens colonizing the epithelial mucosa. They cause primarily atypical pneumonia and have recently been associated with chronic diseases. Diagnostics rely almost exclusively on serological methods; PCR tests are used rarely because in patients with positive ELISA, it is nearly impossible to identify chlamydial DNA. To understand this issue, we elaborated a reliable and sensitive nested PCR method (panNPCR) for identifying all Chlamydiales species, not only in sputa, but also in clotted blood. Sequencing of the PCR product revealed that 41% of positive sputa samples and 66% of positive blood samples were not infected by Chlamydia but with “Chlamydia-related bacteria” such as Rhabdochlamydia sp., Parachlamydia sp., Protochlamydia sp., Neochlamydia sp., Mesochlamydia elodeae and lacustris, Piscichlamydia salmonis, and Estrella lausannensis. Consequently, we propose that there might be more than four human pathogenic Chlamydia species. We did not find any clear correlation between increased levels of antibodies and the presence of their DNA. Chlamydialles DNA was found in sputa samples from individuals positive for IgG or IgA but not in blood samples. Thus, elevated IgG and IgA levels are not reliable markers of chronic infection, and the presence of persistent forms should be proved by panNPCR. Apparently, the differences between ELISA and DNA amplification results have three main methodological reasons. The first one is the threshold occurrence of chlamydial genetic material in sputum and blood. The second one is the fact that a significant part of the samples can have DNA with sequences different from those of other species of the order Chlamydiales. The third one is the high background characteristic for ELISA, the absence of paired sera, and the vague interpretation of the gray zone.
Possible Third Step Preventing Conjugation between Different Species of Blepharisma
Authors: Sugino Ayu , Kobayashi Mayumi , Sugiura Mayumi , Harumoto Terue
Published: January 2023

In the genus Blepharisma, reproductive isolation between different species appears to be established at least by two barriers: (1) a mating pheromone, i.e., gamone 1, and (2) a factor involved in pair formation. Using four species, we experimentally investigated other potential barriers to interspecific conjugation in Blepharisma, as well as the first and second barriers. Cell-free fluid from type I cells (CFF1) of B. americanum had no effect on B. undulans, B. japonicum, or B. stoltei. Type II cells of B. americanum responded to CFF1 from B. americanum but not to CFF1 from B. undulans, B. japonicum, or B. stoltei. Gamone 1, therefore, seems to be the first reproductive barrier (with the inclusion of B. americanum species [megakaryotype 3]) as reported previously. In pretreated cells with complementary gamones in B. undulans and B. americanum, inter-species pair formation was rare, but pair formation between B. americanum and B. japonicum and between B. americanum and B. stoltei occurred at relatively high frequency. Most of the inter-species B. americanum–B. stoltei pairs underwent nuclear changes specific to conjugation. No significant difference was observed between the intra- and inter-species pairs over the time course of the nuclear changes, but the percentage of abnormal cells was higher in inter-species pairs than in intra-species pairs, and no progenies were produced by inter-species pairs. These results suggest a third barrier or step, in addition to the first and second ones, in nuclear changes after pair formation that prevents interspecific conjugation in Blepharisma.
Conjugative RP4 Plasmid-Mediated Transfer of Antibiotic Resistance Genes to Commensal and Multidrug-Resistant Enteric Bacteria In Vitro
Authors: Sher Azam A. , VanAllen Mia E. , Ahmed Husnain , Whitehead-Tillery Charles , Rafique Sonia , Bell Julia A. , Zhang Lixin , Mansfield Linda S.
Published: January 2023

Many antibiotic-resistant bacteria carry resistance genes on conjugative plasmids that are transferable to commensals and pathogens. We determined the ability of multiple enteric bacteria to acquire and retransfer a broad-host-range plasmid RP4. We used human-derived commensal Escherichia coli LM715-1 carrying a chromosomal red fluorescent protein gene and green fluorescent protein (GFP)-labeled broad-host-range RP4 plasmid with ampR, tetR, and kanR in in vitro matings to rifampicin-resistant recipients, including Escherichia coli MG1655, Dec5α, Vibrio cholerae, Pseudomonas putida, Pseudomonas aeruginosa, Klebsiella pneumoniae, Citrobacter rodentium, and Salmonella Typhimurium. Transconjugants were quantified on selective media and confirmed using fluorescence microscopy and PCR for the GFP gene. The plasmid was transferred from E. coli LM715-1 to all tested recipients except P. aeruginosa. Transfer frequencies differed between specific donor–recipient pairings (10−2 to 10−8). Secondary retransfer of plasmid from transconjugants to E. coli LM715-1 occurred at frequencies from 10−2 to 10−7. A serial passage plasmid persistence assay showed plasmid loss over time in the absence of antibiotics, indicating that the plasmid imposed a fitness cost to its host, although some plasmid-bearing cells persisted for at least ten transfers. Thus, the RP4 plasmid can transfer to multiple clinically relevant bacterial species without antibiotic selection pressure.
Bacterial Communities of Lamiacea L. Medicinal Plants: Structural Features and Rhizosphere Effect
Authors: Zharkova Ekaterina K. , Vankova Anna A. , Selitskaya Olga V. , Malankina Elena L. , Drenova Natalya V. , Zhelezova Alena D. , Khlyustov Vitaliy K. , Belopukhov Sergey L. , Zhevnerov Aleksey V. , Sviridova Ludmila A. , Fomina Tatiana N. , Kozlov Andrey V.
Published: January 2023

Bacterial communities associated with medicinal plants are an essential part of ecosystems. The rhizosphere effect is rather important in the cultivation process. The purpose of the study was to analyze the rhizosphere effect of oregano (Origanum vulgare L.), peppermint (Mentha piperita L.), thyme (Thymus vulgaris L.), creeping thyme (Thymus serpillum L.) and sage (Salvia officinalis L.). To estimate the quantity of 16S bacteria ribosomal genes, qPCR assays were used. To compare bacterial communities’ structure of medicinal plants rhizosphere with bulk soil high-throughput sequencing of the 16S rRNA targeting variable regions V3–V4 of bacteria was carried out. The highest bacterial abundance was associated with T. vulgaris L., M. piperita L. and S. officinalis L., and the lowest was associated with the O. vulgare L. rhizosphere. Phylum Actinobacteriota was predominant in all rhizosphere samples. The maximum bacterial α-diversity was found in S. officinalis L. rhizosphere. According to bacterial β-diversity calculated by the Bray–Curtis metric, T. vulgaris L. root zone significantly differed from bulk soil. The rhizosphere effect was positive to the Myxococcota, Bacteroidota, Verrucomicrobiota, Proteobacteria and Gemmatimonadota.
Gold Extraction from a Refractory Sulfide Concentrate by Simultaneous Pressure Leaching/Oxidation
Authors: Soto-Uribe Juan Carlos , Valenzuela-Garcia Jesus Leobardo , Salazar-Campoy Maria Mercedes , Parga-Torres Jose Refugio , Tiburcio-Munive Guillermo , Encinas-Romero Martin Antonio , Vazquez-Vazquez Victor Manuel
Published: January 2023

Most gold deposits occur associated with sulphides like pyrite and arsenopyrite; thus, precious metal dissolution is possible by oxidizing auriferous sulfide concentrate using simultaneous pressure oxidation and cyanidation. The effectiveness of this process of extraction can be influenced by the temperature, cyanide (NaCN) concentration, and oxygen pressure. In this study, we conducted experiments to characterize the effects on gold extraction of ores using a range of sodium cyanide concentrations (1–8 g/L), temperatures (40–75 °C), and oxygen pressures (0.5–1.1 MPa). Characterization of the ores showed that pyrite and quartz were the main minerals present in the concentrate. The best results in terms of the highest extraction of Au were obtained with an oxygen pressure of 0.5 MPa, 6 g/L sodium cyanide, and a temperature of 75 °C, along with a constant stirring speed of 600 rpm. These conditions allowed for approximately 95% gold extraction in 90 min.
GLP-1R Signaling and Functional Molecules in Incretin Therapy
Authors: Wan Wenwei , Qin Qikai , Xie Linshan , Zhang Hanqing , Wu Fan , Stevens Raymond C. , Liu Yan
Published: January 2023

Glucagon-like peptide-1 receptor (GLP-1R) is a critical therapeutic target for type 2 diabetes mellitus (T2DM). The GLP-1R cellular signaling mechanism relevant to insulin secretion and blood glucose regulation has been extensively studied. Numerous drugs targeting GLP-1R have entered clinical treatment. However, novel functional molecules with reduced side effects and enhanced therapeutic efficacy are still in high demand. In this review, we summarize the basis of GLP-1R cellular signaling, and how it is involved in the treatment of T2DM. We review the functional molecules of incretin therapy in various stages of clinical trials. We also outline the current strategies and emerging techniques that are furthering the development of novel therapeutic drugs for T2DM and other metabolic diseases.
Research of Liquid-Crystal Materials for a High-Performance FFS-TFT Display
Authors: Chen Haiguang , Liu Youran , Chen Maoxian , Jiang Tianmeng , Zhang Lanying , Yang Zhou , Yang Huai
Published: January 2023

A novel liquid-crystal compound of more than 99.95% purity with high performance (such as a high clearing point, large dielectric anisotropy, high optical anisotropy, low viscosity, and large elastic constants) was designed and synthesized according to the fringe-field switching thin-film-transistor-liquid-crystal display requirements (FFS-TFT). Then, a mixed liquid-crystal material suitable for an FFS-TFT display was developed by mixing this compound with other reported compounds, developing a product whose quality was that of the highest level of similar foreign products and which fully met the customer’s use requirements (BOE), and thus able to completely replace similar imported materials.
Realizing Scalable Nano-SiO2-Aerogel-Reinforced Composite Polymer Electrolytes with High Ionic Conductivity via Rheology-Tuning UV Polymerization
Authors: Li Mianrui , Qi Shengguang , Li Shulian , Du Li
Published: January 2023

Polymer electrolytes for lithium metal batteries have aroused widespread interest because of their flexibility and excellent processability. However, the low ambient ionic conductivity and conventional fabrication process hinder their large-scale application. Herein, a novel polyethylene-oxide-based composite polymer electrolyte is designed and fabricated by introducing nano-SiO2 aerogel as an inorganic filler. The Lewis acid–base interaction between SiO2 and anions from Li salts facilitates the dissociation of Li+. Moreover, the SiO2 interacts with ether oxygen (EO) groups, which weakens the interaction between Li+ and EO groups. This synergistic effect produces more free Li+ in the electrolyte. Additionally, the facile rheology-tuning UV polymerization method achieves continuous coating and has potential for scalable fabrication. The composite polymer electrolyte exhibits high ambient ionic conductivity (0.68 mS cm−1) and mechanical properties (e.g., the elastic modulus of 150 MPa). Stable lithium plating/stripping for 1400 h in Li//Li symmetrical cells at 0.1 mA cm−2 is achieved. Furthermore, LiFePO4//Li full cells deliver superior discharge capacity (153 mAh g−1 at 0.5 C) and cycling stability (with a retention rate of 92.3% at 0.5 C after 250 cycles) at ambient temperature. This work provides a promising strategy for polymer-based lithium metal batteries.
Small Molecule Drugs That Inhibit Phagocytosis
Authors: Loriamini Melika , Lewis-Bakker Melissa M. , Frias Boligan Kayluz , Wang Siming , Holton Mairead B. , Kotra Lakshmi P. , Branch Donald R.
Published: January 2023

In our initial publication on the in vitro testing of more than 200 compounds, we demonstrated that small molecules can inhibit phagocytosis. We therefore theorized that a small molecule drug discovery-based approach to the treatment of immune cytopenias (ITP, AIHA, HTR, DHTR) is feasible. Those earlier studies showed that small molecules with anti-phagocytic groups, such as the pyrazole core, are good models for producing efficacious phagocytosis inhibitors with low toxicity. We recently screened a chemical library of 80 compounds containing pyrazole/isoxazole/pyrrole core structures and found four hit molecules for further follow-up, all having the pyrazole core structure. Subsequent evaluation via MTT viability, LDH release, and apoptosis, led to the selection of two lead compounds with negligible toxicity and high efficacy. In an in vitro assay for inhibition of phagocytosis, their IC50 values were 2–4 µM. The rational development of these discoveries from hit to lead molecule stage, viz. independent synthesis/scale up of hit molecules, and in vivo activities in mouse models of autoimmune disease, will result in the selection of a lead compound(s) for further pre-clinical evaluation.
A Simple Synthesis of Reduction-Responsive Acrylamide-Type Nanogels for miRNA Delivery
Authors: Maruf Ali , Milewska Małgorzata , Lalik Anna , Student Sebastian , Wandzik Ilona
Published: January 2023

MicroRNAs (miRNAs) have great therapeutic potential; however, their delivery still faces huge challenges, especially given the short half-life of naked miRNAs due to rapid hydrolysis or inactivation by abundant nucleases in the systemic circulation. Therefore, the search for reliable miRNA delivery systems is crucial. Nanogels are one of the more effective nanocarriers because they are biocompatible and have a high drug-loading capacity. In this study, acrylamide-based nanogels containing cationic groups and redox-sensitive crosslinkers were developed for cellular delivery of anti-miR21 (a-miR21). To achieve this, post-polymerization loading of a-miR21 oligonucleotides into nanogels was performed by utilizing the electrostatic interaction between positively charged nanogels and negatively charged oligonucleotides. Different molar ratios of the amine groups (N) on the cationic nanogel and phosphate groups (P) on the miRNA were investigated. An N/P ratio of 2 allowed high miRNA loading capacity (MLC, 6.7% w/w) and miRNA loading efficiency (MLE, 99.7% w/w). Successful miRNA loading was confirmed by dynamic light scattering (DLS) and electrophoretic light scattering (ELS) measurements. miRNA-loaded nanogels (NG/miRNA) formed stable dispersions in biological media and showed an enhanced miRNA release profile in the presence of glutathione (GSH). Moreover, the addition of heparin to dissociate the miRNA from the cationic nanogels resulted in the complete release of miRNA. Lastly, a cell uptake study indicated that NG/miRNA could be easily taken up by cancer cells.
3-(Adenosylthio)benzoic Acid Derivatives as SARS-CoV-2 Nsp14 Methyltransferase Inhibitors
Authors: Bobileva Olga , Bobrovs Raitis , Sirma Evelina Elva , Kanepe Iveta , Bula Anna L. , Patetko Liene , Ramata-Stunda Anna , Grinberga Solveiga , Jirgensons Aigars , Jaudzems Kristaps
Published: January 2023

SARS-CoV-2 nsp14 guanine-N7-methyltransferase plays an important role in the viral RNA translation process by catalyzing the transfer of a methyl group from S-adenosyl-methionine (SAM) to viral mRNA cap. We report a structure-guided design and synthesis of 3-(adenosylthio)benzoic acid derivatives as nsp14 methyltransferase inhibitors resulting in compound 5p with subnanomolar inhibitory activity and improved cell membrane permeability in comparison with the parent inhibitor. Compound 5p acts as a bisubstrate inhibitor targeting both SAM and mRNA-binding pockets of nsp14. While the selectivity of 3-(adenosylthio)benzoic acid derivatives against human glycine N-methyltransferase was not improved, the discovery of phenyl-substituted analogs 5p,t may contribute to further development of SARS-CoV-2 nsp14 bisubstrate inhibitors.
The Functional and Physicochemical Properties of Rice Protein Concentrate Subjected to Acetylation
Authors: Miedzianka Joanna , Walkowiak Katarzyna , Zielińska-Dawidziak Magdalena , Zambrowicz Aleksandra , Wolny Szymon , Kita Agnieszka
Published: January 2023

The aim of the present study was to increase the value of rice protein concentrate (RPC) by improving the functional properties of a preparation subjected to acetylation and analyze the impact of this chemical modification on chemical composition, digestibility, and protein patterning using SDS-PAGE electrophoresis and FT-IR spectroscopy. In the modified samples, the protein content increased (80.90–83.10 g/100 g cf. 74.20 g/100 g in the control). Electrophoresis revealed that the content of the main rice protein fractions (prolamin and glutelin) decreased as the concentration of the modifying reagent increased. Through spectroscopic analysis, wavenumbers, corresponding to the presence of proteins or lipids, aromatic systems, and carbohydrates, were observed. The use of acetic anhydride did not change the digestibility of the modified RPC significantly when compared to that of the control sample. The acetylation of the RPC caused a significant increase in its emulsifying properties at pH 8 (1.83–14.74%) and its water-binding capacity but did not have a statistically significant impact on the oil-absorption capacity. There was a slight increase in protein solubility and a decrease in foaming capacity in the modified RPC.
Upscaling of Carbon-Based Perovskite Solar Module
Authors: Stefanelli Maurizio , Vesce Luigi , Di Carlo Aldo
Published: January 2023

Perovskite solar cells (PSCs) and modules are driving the energy revolution in the coming photovoltaic field. In the last 10 years, PSCs reached efficiency close to the silicon photovoltaic technology by adopting low-cost solution processes. Despite this, the noble metal (such as gold and silver) used in PSCs as a counter electrode made these devices costly in terms of energy, CO2 footprint, and materials. Carbon-based perovskite solar cells (C-PSCs) and modules use graphite/carbon-black-based material as the counter electrode. The formulation of low-cost carbon-based inks and pastes makes them suitable for large area coating techniques and hence a solid technology for imminent industrialization. Here, we want to present the upscaling routes of carbon-counter-electrode-based module devices in terms of materials formulation, architectures, and manufacturing processes in order to give a clear vision of the scaling route and encourage the research in this green and sustainable direction.
Phemenology of Filling, Investigation of Growth Kinetics and Electronic Properties for Applications of Filled Single-Walled Carbon Nanotubes
Authors: Kharlamova Marianna V. , Kramberger Christian
Published: January 2023

This review discusses the phemenology of filling, the investigation of kinetics, and the electronic properties for applications of filled single-walled carbon nanotubes (SWCNTs), and summarizes five main achievements that were obtained in processing the spectroscopic data of SWCNTs filled with metal halogenide, metal chalcogenide, metal and metallocenes. First, the methods of processing kinetic data were developed to reveal precise trends in growth rates and activation energies of the growth of SWCNTs. Second, the metal-dependence of kinetics was revealed. Third, metallicity-sorted (metallic and semiconducting) SWCNTs were filled with a range of substances and the electronic properties were investigated. Fourth, new approaches to processing the data of spectroscopic investigations of filled SWCNTs were developed, which allowed more reliable and precise analysis of the experimental results. Fifth, the correlation between the physical and chemical properties of encapsulated substances and the electronic properties of SWCNTs were elucidated. These points are highlighted in the review.
Flexible and Stretchable Carbon-Based Sensors and Actuators for Soft Robots
Authors: Zhou Xinyi , Cao Wenhan
Published: January 2023

In recent years, the emergence of low-dimensional carbon-based materials, such as carbon dots, carbon nanotubes, and graphene, together with the advances in materials science, have greatly enriched the variety of flexible and stretchable electronic devices. Compared with conventional rigid devices, these soft robotic sensors and actuators exhibit remarkable advantages in terms of their biocompatibility, portability, power efficiency, and wearability, thus creating myriad possibilities of novel wearable and implantable tactile sensors, as well as micro-/nano-soft actuation systems. Interestingly, not only are carbon-based materials ideal constituents for photodetectors, gas, thermal, triboelectric sensors due to their geometry and extraordinary sensitivity to various external stimuli, but they also provide significantly more precise manipulation of the actuators than conventional centimeter-scale pneumatic and hydraulic robotic actuators, at a molecular level. In this review, we summarize recent progress on state-of-the-art flexible and stretchable carbon-based sensors and actuators that have creatively added to the development of biomedicine, nanoscience, materials science, as well as soft robotics. In the end, we propose the future potential of carbon-based materials for biomedical and soft robotic applications.
Solvent-Free Processed Cathode Slurry with Carbon Nanotube Conductors for Li-Ion Batteries
Authors: Park Gyori , Kim Hyun-Suk , Lee Kyung Jin
Published: January 2023

The increase in demand for energy storage devices, including portable electronic devices, electronic mobile devices, and energy storage systems, has led to substantial growth in the market for Li-ion batteries (LiB). However, the resulting environmental concerns from the waste of LiB and pollutants from the manufacturing process have attracted considerable attention. In particular, N-methylpyrrolidone, which is utilized during the manufacturing process for preparing cathode or anode slurries, is a toxic organic pollutant. Therefore, the dry-based process for electrodes is of special interest nowadays. Herein, we report the fabrication of a cathode by a mortar-based dry process using NCM811, a carbon conductor, and poly(tetrafluoroethylene)binder. The electrochemical performance of the cathode was compared in terms of the types of conductors: carbon nanotubes and carbon black. The electrodes with carbon nanotubes showed an ameliorated performance in terms of cycle testing, capacity retention, and mechanical properties.
Effects of Pretreatment and Polarization Shielding on EK-PRB of Fe/Mn/C-LDH for Remediation of Arsenic Contaminated Soils
Authors: Zhu Zongqiang , Kong Yusong , Yang Hongqu , Tian Yan , Zhou Xiaobin , Zhu Yinian , Fang Zhanqiang , Zhang Lihao , Tang Shen , Fan Yinming
Published: January 2023

In this study, coupling electrokinetic (EK) with the permeable reactive barriers (PRB) of Fe/Mn/C-LDH composite was applied for the remediation of arsenic-contaminated soils. By using self-made Fe/Mn/C-LDH materials as PRB filler, the effects of pretreatment and polarization shielding on EK-PRB of Fe/Mn/C-LDH for remediation of arsenic contaminated soils were investigated. For the pretreatment, phosphoric acid, phosphoric acid and water washing, and phosphate were adopted to reduce the influence of iron in soil. The addition of phosphate could effectively reduce the soil leaching toxicity concentration. The removal rate of the soil pretreated with phosphoric acid or phosphoric acid and water washing was better than with phosphate pretreatment. For the polarization shielding, circulating electrolyte, electrolyte type, anion and cation membranes, and the exchange of cathode and anode were investigated. The electrolyte circulates from the cathode chamber to the anode chamber through the peristaltic pump to control the pH value of the electrolyte, and the highest arsenic toxicity removal rate in the soil reaches 97.36%. The variation of total arsenic residue in soil using anion and cation membranes is the most regular. The total arsenic residue gradually decreases from cathode to anode. Electrode exchange can neutralize H+ and OH− produced by electrolyte, reduce the accumulation of soil cathode area, shield the reduction of repair efficiency caused by resistance polarization, enhance current, and improve the removal rate of arsenic in soil.
Analysis of Fecal Short-Chain Fatty Acids (SCFAs) in Healthy Children during the First Two Years of Life: An Observational Prospective Cohort Study
Authors: Łoniewska Beata , Fraszczyk-Tousty Magda , Tousty Piotr , Skonieczna-Żydecka Karolina , Maciejewska-Markiewicz Dominika , Łoniewski Igor
Published: January 2023

Short-chain fatty acids (SCFAs) are important metabolites of the gut microbiota. The aim is to analyze the influence of perinatal factors, which can affect the gut microbiota, on the concentrations of fecal SCFAs over the first two years of life. Gas chromatography was used to analyze SCFA in a total of 456 fecal samples from 86 children. Total SCFA concentrations increased until 12 months and stabilized after that. Antibiotic treatment during pregnancy was associated with an increase in acetic acid, propionic acid and total SCFA in meconium and a decrease in the same SCFAs at 6 months. Butyric acid was increased after Caesarean delivery until 1 month. In formula-fed children, propionic acid (at 1 month) and butyric acid and total SCFA (at 12 months) were increased. Acetic and linear butyric acids and total SCFAs were also increased at 12 months in children born vaginally that were also formula-fed. Higher butyric acid was observed in children of mothers with normal pre-pregnancy weight and adequate weight gain during pregnancy. Butyric acid was also elevated in 6-month-old infants with a higher body weight (≥85th percentile). Acetic acid concentrations were significantly higher in 2-year-old females vs. males. We conclude that perinatal factors are linked to changes in fecal SCFAs and further long-term epidemiological studies are warranted.