bluuebunny/crossref_metadata_2025 huggingface dataset:
Glucocorticoid Receptor Isoforms in Breast Cancer Raise Implications for Personalised Supportive Therapies
Authors: Butz Henriett , Vereczki Viktória , Budai Barna , Rubovszky Gábor , Gyebrovszki Rebeka , Vida Ramóna , Szőcs Erika , Gerecs Bence , Kohánka Andrea , Tóth Erika , Likó István , Kacskovics Imre , Patócs Attila
Published: November 2024

Glucocorticoid receptor (GR) activation may promote metastasis in oestrogen receptor-negative and triple-negative breast cancer (TNBC). However, the role of the GRβ isoform, which has opposing effects to the main isoform, has not been studied in clinical samples. We aimed to analyse the intracellular localisation of total GR and GRβ in vitro using plasmid constructs and fluorescent immunocytochemistry. Additionally, our goal was to perform immunostaining for total GR and GRβ on two cohorts: (i) on 194 clinical breast cancer samples to compare the expression in different molecular subtypes, and (ii) on 161 TNBC samples to analyse the association of GR with survival. We supplemented our analysis with RNA data from 1097 TNBC cases. We found that in the absence of the ligand, GR resided in the cytoplasm of breast cancer cells, while upon ligand activation, it translocated to the nucleus. A negative correlation was found between cytoplasmic GRtotal and Ki67 in luminal A tumours, while the opposite trend was observed in TNBC samples. Tumours with strong lymphoid infiltration showed higher cytoplasmic GRtotal staining compared to those with weaker infiltration. Patients with high nuclear GRtotal staining had shorter progression-free survival in univariate analysis. High cytoplasmic GRβ was a marker for better overall survival in multivariate analysis (10-year overall survival HR [95% CI]: 0.46 [0.22–0.95], p = 0.036). As a conclusions, this study is the first to investigate GRβ expression in breast tumours. Different expression and cellular localisation of GRtotal and GRβ were observed in the context of molecular subtypes, underscoring the complex role of GR in breast cancer. An inverse association between cytoplasmic GRtotal and the Ki67 proliferation index was observed in luminal A and TNBC. Regarding the impact of GR on outcomes in TNBC patients, while cytoplasmic GRβ was associated with a better prognosis, patients with nuclear GRtotal staining may be at a higher risk of disease progression, as it negatively affects survival. Caution should be exercised when using glucocorticoids in patients with nuclear GR staining, as it may negatively impact survival.
ROS as Signaling Molecules to Initiate the Process of Plant Acclimatization to Abiotic Stress
Authors: Fedoreyeva Larisa Ivanovna
Published: November 2024

During their life cycle, plants constantly respond to environmental changes. Abiotic stressors affect the photosynthetic and respiratory processes of plants. Reactive oxygen species (ROS) are produced during aerobic metabolism and play an important role as regulatory mediators in signaling processes, activating the plant’s protective response to abiotic stress and restoring “oxidation-reduction homeostasis”. Cells develop normally if the rates of ROS production and the ability to neutralize them are balanced. To implement oxidation-reduction signaling, this balance must be disrupted either by an increase in ROS concentration or a decrease in the activity of one or more antioxidant systems. Under abiotic stress, plants accumulate excessive amounts of ROS, and if the ROS content exceeds the threshold amount dangerous for living organisms, it can lead to damage to all major cellular components. Adaptive resistance of plants to abiotic stressors depends on a set of mechanisms of adaptation to them. The accumulation of ROS in the cell depends on the type of abiotic stress, the strength of its impact on the plant, the duration of its impact, and the recovery period. The aim of this review is to provide a general understanding of the processes occurring during ROS homeostasis in plants, oxidation-reduction processes in cellular compartments in response to abiotic stress, and the participation of ROS in signaling processes activating adaptation processes to abiotic stress.
Phytochemical Composition and Functional Properties of Brassicaceae Microgreens: Impact of In Vitro Digestion
Authors: Šola Ivana , Vujčić Bok Valerija , Popović Maja , Gagić Sanja
Published: November 2024

The aim of this study was to compare the concentration of phenolic compounds, glucosinolates, proteins, sugars and vitamin C between kohlrabi (Brassica oleracea var. acephala gongylodes), Savoy cabbage (B. oleracea sabauda), Brussels sprouts (B. oleracea gemmifera), cauliflower (B. oleracea botrytis), radish (Raphanus sativus) and garden cress (Lepidium sativum) microgreens for their antioxidant and hypoglycemic potential. In addition, we applied an in vitro-simulated system of human digestion in order to track the bioaccessibility of the selected phenolic representatives, and the stability of the microgreens’ antioxidant and hypoglycemic potential in terms of α-amylase and α-glucosidase inhibition after each digestion phase. Using spectrophotometric and RP-HPLC methods with statistical analyses, we found that garden cress had the lowest soluble sugar content, while Savoy cabbage and Brussels sprouts had the highest glucosinolate levels (76.21 ± 4.17 mg SinE/g dm and 77.73 ± 3.33 mg SinE/g dm, respectively). Brussels sprouts were the most effective at inhibiting protein glycation (37.98 ± 2.30% inhibition). A very high positive correlation (r = 0.830) between antiglycation potential and conjugated sinapic acid was recorded. For the first time, the antidiabetic potential of microgreens after in vitro digestion was studied. Kohlrabi microgreens best inhibited α-amylase in both initial and intestinal digestion (60.51 ± 3.65% inhibition and 62.96 ± 3.39% inhibition, respectively), and also showed the strongest inhibition of α-glucosidase post-digestion (19.22 ± 0.08% inhibition). Brussels sprouts, cauliflower, and radish had less stable α-glucosidase than α-amylase inhibitors during digestion. Kohlrabi, Savoy cabbage, and garden cress retained inhibition of both enzymes after digestion. Kohlrabi antioxidant capacity remained unchanged after digestion. The greatest variability was seen in the original samples, while the intestinal phase resulted in the most convergence, indicating that digestion reduced differences between the samples. In conclusion, this study highlights the potential of various microgreens as sources of bioactive compounds with antidiabetic and antiglycation properties. Notably, kohlrabi microgreens demonstrated significant enzyme inhibition after digestion, suggesting their promise in managing carbohydrate metabolism and supporting metabolic health.
Transcriptome and Expression Analysis of Glycerol Biosynthesis-Related Genes in Glenea cantor Fabricius (Cerambycidae: Lamiinae)
Authors: Lan Taihui , Su Ranran , Dong Zishu , Tong Xin , Zheng Xialin , Wang Xiaoyun
Published: November 2024

Glenea cantor Fabricius (Cerambycidae: Lamiinae) is an important pest that damages kapok trees in Southeast Asia with a wide adaptability to temperature. Glycerol is a protectant and energy source for insects in low-temperature environments. However, glycerol biosynthesis-related genes at the molecular level are limited in G. cantor. In this study, the supercooling points and freezing points at different stages were measured, and the cold hardiness of male and female pupae significantly differed. Moreover, a full-length transcriptome of G. cantor was established; glycerol kinase (GK) and glycerol-3-phosphate dehydrogenase (GPDH) genes, which are related to glycerol metabolism, were identified, with a special focus on their expression profiles. A total of 24,476 isoforms stemmed from the full-length transcriptome, along with 568 lncRNAs, 56 transcription factor (TF) families, and 1467 alternative splicing (AS) events. The KEGG pathway enrichment analysis revealed that the isoforms associated with AS were enriched primarily in glycerolipid and glycerophospholipid metabolism. In total, three GK genes and one GPDH gene were identified, and GcGK1 and GcGK3 presented differential sex expression during the pupal stage, which may play a role in thermal adaptability. This study provides a valuable transcriptional database of G. cantor and helps to elucidate the function of glycerol in the thermal adaptation mechanism of longhorn beetles.
Ten Hypermethylated lncRNA Genes Are Specifically Involved in the Initiation, Progression, and Lymphatic and Peritoneal Metastasis of Epithelial Ovarian Cancer
Authors: Braga Eleonora A. , Burdennyy Alexey M. , Uroshlev Leonid A. , Zaichenko Danila M. , Filippova Elena A. , Lukina Svetlana S. , Pronina Irina V. , Astafeva Iana R. , Fridman Marina V. , Kazubskaya Tatiana P. , Loginov Vitaly I. , Dmitriev Alexey A. , Moskovtsev Aleksey A. , Kushlinskii Nikolay E.
Published: November 2024

Abstract: Our work aimed to evaluate and differentiate the role of ten lncRNA genes (GAS5, HAND2-AS1, KCNK15-AS1, MAGI2-AS3, MEG3, SEMA3B-AS1, SNHG6, SSTR5-AS1, ZEB1-AS1, and ZNF667-AS1) in the development and progression of epithelial ovarian cancer (EOC). A representative set of clinical samples was used: 140 primary tumors from patients without and with metastases and 59 peritoneal metastases. Using MS-qPCR, we demonstrated an increase in methylation levels of all ten lncRNA genes in tumors compared to normal tissues (p < 0.001). Using RT-qPCR, we showed downregulation and an inverse relationship between methylation and expression levels for ten lncRNAs (rs < −0.5). We further identified lncRNA genes that were specifically hypermethylated in tumors from patients with metastases to lymph nodes (HAND2-AS1), peritoneum (KCNK15-AS1, MEG3, and SEMA3B-AS1), and greater omentum (MEG3, SEMA3B-AS1, and ZNF667-AS1). The same four lncRNA genes involved in peritoneal spread were associated with clinical stage and tumor extent (p < 0.001). Interestingly, we found a reversion from increase to decrease in the hypermethylation level of five metastasis-related lncRNA genes (MEG3, SEMA3B-AS1, SSTR5-AS1, ZEB1-AS1, and ZNF667-AS1) in 59 peritoneal metastases. This reversion may be associated with partial epithelial–mesenchymal transition (EMT) in metastatic cells, as indicated by a decrease in the level of the EMT marker, CDH1 mRNA (p < 0.01). Furthermore, novel mRNA targets and regulated miRNAs were predicted for a number of the studied lncRNAs using the NCBI GEO datasets and analyzed by RT-qPCR and transfection of SKOV3 and OVCAR3 cells. In addition, hypermethylation of SEMA3B-AS1, SSTR5-AS1, and ZNF667-AS1 genes was proposed as a marker for overall survival in patients with EOC.
Advancements in Therapeutic Approaches for Degenerative Tendinopathy: Evaluating Efficacy and Challenges
Authors: Morya Vivek Kumar , Shahid Hamzah , Lang Jun , Kwak Mi Kyung , Park Sin-Hye , Noh Kyu-Cheol
Published: November 2024

Degenerative tendinopathy results from the accumulation of minor injuries following unsuccessful tendon repair during acute tendon injuries. The process of tendon repair is prolonged and varies between individuals, making it susceptible to reinjury. Moreover, treating chronic tendinopathy often requires expensive and extensive rehabilitation, along with a variety of combined therapies to facilitate recovery. This condition significantly affects the quality of life of affected individuals, underscoring the urgent need for more efficient and cost-effective treatment options. Although traditional treatments have improved significantly and are being used as substitutes for surgical interventions, the findings have been inconsistent and conflicting. This review aims to clarify these issues by exploring the strengths and limitations of current treatments as well as recent innovations in managing various forms of degenerative tendinopathy.
Molecular and Cellular Mechanisms Involved in the Pathophysiology of Retinal Vascular Disease—Interplay Between Inflammation and Oxidative Stress
Authors: Srejovic Jovana V. , Muric Maja D. , Jakovljevic Vladimir Lj. , Srejovic Ivan M. , Sreckovic Suncica B. , Petrovic Nenad T. , Todorovic Dusan Z. , Bolevich Sergey B. , Sarenac Vulovic Tatjana S.
Published: November 2024

Retinal vascular diseases encompass several retinal disorders, including diabetic retinopathy, retinopathy of prematurity, age-related macular degeneration, and retinal vascular occlusion; these disorders are classified as similar groups of disorders due to impaired retinal vascularization. The aim of this review is to address the main signaling pathways involved in the pathogenesis of retinal vascular diseases and to identify crucial molecules and the importance of their interactions. Vascular endothelial growth factor (VEGF) is recognized as a crucial and central molecule in abnormal neovascularization and a key phenomenon in retinal vascular occlusion; thus, anti-VEGF therapy is now the most successful form of treatment for these disorders. Interaction between angiopoietin 2 and the Tie2 receptor results in aberrant Tie2 signaling, resulting in loss of pericytes, neovascularization, and inflammation. Notch signaling and hypoxia-inducible factors in ischemic conditions induce pathological neovascularization and disruption of the blood–retina barrier. An increase in the pro-inflammatory cytokines—TNF-α, IL-1β, and IL-6—and activation of microglia create a persistent inflammatory milieu that promotes breakage of the blood–retinal barrier and neovascularization. Toll-like receptor signaling and nuclear factor-kappa B are important factors in the dysregulation of the immune response in retinal vascular diseases. Increased production of reactive oxygen species and oxidative damage follow inflammation and together create a vicious cycle because each factor amplifies the other. Understanding the complex interplay among various signaling pathways, signaling cascades, and molecules enables the development of new and more successful therapeutic options.
Evaluation of a Novel Mechanical Device for the Production of Microfragmented Adipose Tissue for Veterinary Regenerative Medicine: A Proof-of-Concept
Authors: Berni Priscilla , Andreoli Valentina , Conti Virna , Ramoni Roberto , Basini Giuseppina , Scattini Gabriele , Pascucci Luisa , Pellegrini Martina , Del Bue Maurizio , Squassino Gian Paolo , Paino Francesca , Pessina Augusto , Alessandri Giulio , Pirazzoli Paolo , Bosetto Antonio , Grolli Stefano
Published: November 2024

Therapies based on mesenchymal stromal cells (MSCs) have become one of the most significant advancements in veterinary regenerative medicine. The isolation of MSCs is usually performed by enzymatic digestion and requires variable times for cell expansion. In addition, these procedures need to be performed in specialized laboratory facilities. An alternative approach to in vitro-expanded MSC therapy is the use of microfragmented adipose tissue (microfat), which is a rich source of cells and growth factors from the stromal vascular fraction. Recent clinical studies support its safety and efficacy in the treatment of musculoskeletal disorders and wound healing. The aim of the present work was to characterize the microfragmented adipose tissue obtained by a new mechanical device, which provides sterile tissue that is ready for use in the clinic by the veterinarian, avoiding the need for specialized laboratory facilities. Microfat-derived MSCs were compared with enzymatically isolated MSCs in terms of their phenotypic characterization, growth rate and differentiation potential. Conditioned medium derived from microfat culture was evaluated for its ability to promote MSC vitality. No differences were observed between MSCs obtained through mechanical fragmentation and those derived from collagenase digestion of adipose tissue, suggesting that the device could serve as a practical source of microfragmented adipose tissue for use in veterinary clinics.
Public Health Using Social Network Analysis During the COVID-19 Era: A Systematic Review
Authors: Gardasevic Stanislava , Jaiswal Aditi , Lamba Manika , Funakoshi Jena , Chu Kar-Hai , Shah Aekta , Sun Yinan , Pokhrel Pallav , Washington Peter
Published: November 2024

Social network analysis (SNA), or the application of network analysis techniques to social media data, is an increasingly prominent approach used in computational public health research. We conducted a systematic review to investigate trends around SNA applied to social media data for public health and epidemiology while outlining existing ethical practices. Following PRISMA guidelines, we reviewed articles from Web of Science and PubMed published between January 2019 and February 2024, leading to a total of 51 papers surveyed. The majority of analyzed research (69%) involved studying Twitter/X, followed by Sina Weibo (16%). The most prominent topics in this timeframe were related to COVID-19, while other papers explored public health topics such as citizen science, public emergencies, behavior change, and various medical conditions. We surveyed the methodological approaches and network characteristics commonly employed in public health SNA studies, finding that most studies applied only basic network metrics and algorithms such as layout, community detection, and standard centrality measures. We highlight the ethical concerns related to the use of social media data, such as privacy and consent, underscoring the potential of integrating ethical SNA with more inclusive, human-centered practices to enhance the effectiveness and community buy-in of emerging computational public health efforts.
Early Estimation in Agile Software Development Projects: A Systematic Mapping Study
Authors: Rivera Ibarra José Gamaliel , Borrego Gilberto , Palacio Ramón R.
Published: November 2024

Estimating during the early stages is crucial for determining the feasibility and conducting the budgeting and planning of agile software development (ASD) projects. However, due to the characteristics of ASD and limited initial information, these estimates are often complicated and inaccurate. This study aims to systematically map the literature to identify the most used estimation techniques; the reasons for their selection; the input artifacts, predictors, and metrics associated with these techniques; as well as research gaps in early-stage estimations in ASD. This study was based on the guidelines proposed by Kitchenham for systematic literature reviews in software engineering; a review protocol was defined with research questions and criteria for the selection of empirical studies. Results show that data-driven techniques are preferred to reduce biases and inconsistencies of expert-driven techniques. Most selected studies do not mention input artifacts, and software size is the most commonly used predictor. Machine learning-based techniques use publicly available data but often contain records of old projects from before the agile movement. The study highlights the need for tools supporting estimation activities and identifies key areas for future research, such as evaluating hybrid approaches and creating datasets of recent projects with sufficient contextual information and standardized metrics.
Hospitalizations of Patients with Atrial Fibrillation (AF) in Poland: A Nationwide Study Based on over One Million AF Hospitalizations in 2017–2021
Authors: Dąbrowska Martyna , Rząd Michał , Kanecki Krzysztof , Lewtak Katarzyna , Tyszko Piotr , Goryński Paweł , Nitsch-Osuch Aneta
Published: November 2024

Background/Objectives: Atrial fibrillation (AF) is one of the most common forms of persistent arrhythmia in adults worldwide. The aim of this study was to present recent data on the epidemiology of patients hospitalized with AF in Poland. Methods: This is a retrospective, population-based study conducted using hospital discharge records. The data covered 1,225,424 cases of AF hospitalization reported in 2017–2021. Results: The study group consisted of 51.36% men and 48.64% women. The mean and median ages were 73.6 and 74 years, respectively. Women were older than men (77 vs. 70 years, p < 0.001). The mean and median lengths of hospitalization were 6.9 and 4 days. The mean annual hospitalization rate was 640.0 per 100,000 person-years. In the group of patients aged ≥65 years, the hospitalization rate was 2870.4 per 100,000 person-years. Men were hospitalized more frequently than women (p < 0.001). The total in-hospital mortality rate was 37.7 per 100,000 person-years, and it was higher in women than in men (p < 0.001). There was a significant downward trend in first-time hospitalizations during the analyzed period and a significant downward trend in mortality rates with a marked increase in the years 2020–2021. Conclusions: Although women are less frequently hospitalized for AF, they show a higher risk of fatal hospitalizations. The pandemic may have reduced new AF diagnoses and increased mortality in this group of patients. The results of this study may be helpful in making comparative analyses in the European and global contexts and taking actions aimed at improving the health condition of the Polish population.
A Proton Pump Inhibitor Independently Elevates Gastrin Levels as a Marker for Metachronous Gastric Cancer After Endoscopic Submucosal Dissection
Authors: Teshima Hajime , Takigawa Hidehiko , Kotachi Takahiro , Tsuboi Akiyoshi , Tanaka Hidenori , Yamashita Ken , Kishida Yoshihiro , Urabe Yuji , Kuwai Toshio , Ishikawa Akira , Oka Shiro
Published: November 2024

Background and Objective: Serum markers such as gastrin and pepsinogen are useful for stratifying gastric cancer risk. However, their utility in predicting metachronous gastric cancer after endoscopic submucosal dissection (ESD) in patients with gastric cancer after Helicobacter pylori eradication (GCAE) is unclear. This study aimed to clarify predictive factors for metachronous gastric cancer after ESD with a focus on serum markers. Methods: A retrospective analysis was conducted on 197 patients with 224 GCAE lesions who underwent ESD at Hiroshima University Hospital between April 2010 and December 2019. In total, 63 patients with 74 differentiated-type lesions were classified into metachronous gastric cancer (MG) and non-metachronous gastric cancer (NMG) groups, excluding proton pump inhibitor (PPI) users, female patients, and undifferentiated-type cases. The predictive value of serum markers was assessed using ROC curve analysis, and their association with carcinogenesis was evaluated using multiple logistic regression. Furthermore, the incidence of MG was compared between long-term PPI users and non-users. Results: ROC analysis revealed that serum gastrin had the highest discriminative ability for MG (AUC 0.77, cut-off 99 pg/mL, sensitivity 61.6%, and specificity 80.0%). Severe mucosal atrophy and high gastrin levels were significantly more common in the MG group and were independent predictors (p < 0.01). Although serum gastrin levels were significantly elevated in PPI users, no increased risk of MG was observed. Conclusions: In addition to severe mucosal atrophy, PPI-independent elevated serum gastrin levels may be associated with an increased risk of MG after ESD. Serum gastrin may serve as a valuable marker for post-ESD cancer surveillance in GCAE patients.
Diagnosis of Enlarged Vestibular Aqueduct Using Wideband Tympanometry
Authors: Ganaha Akira , Nojiri Nao , Nakamura Takeshi , Higa Teruyuki , Kondo Shunsuke , Tono Tetsuya
Published: November 2024

Background: Wideband tympanometry (WBT) has the potential to distinguish various mechanical middle ear and inner ear pathologies noninvasively. This study investigated the diagnostic value of WBT in the diagnosis of enlarged vestibular aqueduct (EVA). Methods: The absorbance and resonance frequency (RF) of patients with EVA (40 ears, 25 patients) and matched population controls (39 ears, 28 subjects) were compared, alongside receiver operating characteristic (ROC) analysis. Correlations between VA width and RF were also examined. Results: Patients with EVA had higher absorbance at low frequencies (226–917 Hz) and lower absorbance at high frequencies (2520–4896 Hz) compared to controls. The RF of the EVA group was significantly lower versus controls (751 [391–1165] vs. 933 [628–1346] Hz). The ROC analysis revealed area under the curve values of 0.771 and 0.801, respectively, for absorbance and RF. RF had a sensitivity, specificity, positive predictive value, and negative predictive value of 74.4%, 82.5%, 76.7%, and 80.6%, respectively, for diagnosing EVA. In the EVA group, the VA midpoint width (r = −0.334) and VA petrous width (r = −0.402) both significantly correlated with RF. Conclusions: Our findings support the utility of WBT for diagnosing EVA, with RF as the optimal index used.
Activation of Irrigants in Root Canals with Open Apices: A Narrative Review
Authors: Abdellatif Dina , Pisano Massimo , Cecere Luigi , Natoli Valentino , Mancino Davide , Euvrard Eduard , Iandolo Alfredo
Published: November 2024

Performing endodontic treatment on a tooth with an immature apex can be challenging due to the risk of irrigant extrusion beyond the apex. This narrative review investigates the over-apex extrusion of activated irrigants in teeth with open and immature apices and aims to provide crucial insights for practice and research. Two hundred fifty-two results were found from the electronic search. Sixteen duplicates were removed before selection, and 24 were excluded. Of the 212 remaining results, the full text was evaluated for eligibility. One hundred ninety-four results were excluded: in one hundred sixty-five, irrigant activation was not performed; twenty-nine were animal studies; in seven, irrigant activation was not performed to disinfect the canal. Finally, 13 studies were included. They cover a range of topics, from the types of irrigants used to the techniques of activation, and their findings contribute to our understanding of the risks and consequences of irrigant extrusion. All the activation techniques investigated can, to varying degrees, cause the irrigant to extrude beyond the apex. Extrusion may not always be clinically relevant; however, the consequences of excessive irrigant leakage from the apex are dangerous, so try to assess all the variables that may cause it and adopt techniques to reduce it.
Applications of Near Infrared Spectroscopy and Mirror Therapy for Upper Limb Rehabilitation in Post-Stroke Patients: A Brain Plasticity Pilot Study
Authors: Formica Caterina , De Salvo Simona , Muscarà Nunzio , Bonanno Lilla , Arcadi Francesca Antonia , Lo Buono Viviana , Acri Giuseppe , Quartarone Angelo , Marino Silvia
Published: November 2024

Objectives: The aim of this study was to identify the neural pattern activation during mirror therapy (MT) and explore any cortical reorganization and reducing asymmetry of hemispheric activity for upper limb rehabilitation in post-stroke patients. Methods: A box containing a mirror was placed between the arms of the patients to create the illusion of normal motion in the affected limb by reflecting the image of the unaffected limb in motion. We measured the cerebral hemodynamic response using near-infrared spectroscopy (NIRS). We enrolled ten right-handed stroke patients. They observed healthy hand movements in the mirror (MT condition) while performing various tasks (MT condition), and then repeated the same tasks with the mirror covered (N-MT condition). Results: Significant activation of some brain areas was observed in the right and left hemiparesis groups for the MT condition, while lower levels of activation were observed for the N-MT condition. The results showed significant differences in hemodynamic response based on oxygenated (HbO) concentrations between MT and N-MT conditions across all tasks in sensorimotor areas. These neural circuits were activated despite the motor areas being affected by the brain injury, indicating that the reflection of movement in the mirror helped to activate them. Conclusions: These results suggest that MT promotes cortical activations of sensory motor areas in affected and non-affected brain sides in subacute post-stroke patients, and it encourages the use of these tools in clinical practice.
Complex Thoracic Aortic Diseases and Surgery: A Quest for the Golden Fleece
Authors: Magouliotis Dimitrios E. , Athanasiou Thanos
Published: November 2024

In Greek mythology, the Golden Fleece, a golden-wooled fleece of a winged ram called Chrysomallos, stood as an eternal symbol of authority and power [...]
Comprehensive Review of Hydrogel Synthesis, Characterization, and Emerging Applications
Authors: Priya Arumugasamy Sathiya , Premanand Rajaraman , Ragupathi Indhumathi , Bhaviripudi Vijayabhaskara Rao , Aepuru Radhamanohar , Kannan Karthik , Shanmugaraj Krishnamoorthy
Published: November 2024

Hydrogels play a crucial role due to their high-water content and 3D structure, which make them ideal for various applications in biomedicine, sensing, and beyond. They can be prepared from a variety of biomaterials, polymers, and their combinations, allowing for versatility in properties and applications. Hydrogels include natural types derived from collagen, gelatin, alginate, and hyaluronic acid, as well as synthetic types based on polyethylene glycol (PEG), polyvinyl alcohol (PVA), and polyacrylamide (PAAm). Each type possesses distinct properties, such as mechanical strength, biodegradability, and biocompatibility, which can be tailored for applications such as wound healing, contact lenses, 3D bioprinting, and tissue engineering. The high-water content of hydrogels mimics natural tissue environments, promoting cell growth and allowing nutrient and waste exchange, which supports the development of functional tissues. They serve as scaffolds in tissue engineering applications, including wound healing, cartilage and bone regeneration, vascular tissue engineering, and organ-on-a-chip systems. Additionally, hydrogels can encapsulate and deliver therapeutic agents, such as growth factors or drugs, to specific target sites in the body. Hydrogels can be prepared through three primary methods: physical crosslinking, which relies on non-covalent interactions such as physical entanglements or hydrogen bonding; chemical crosslinking, which forms covalent bonds between polymer chains to create a stable structure; and irradiation-based crosslinking, where UV irradiation induces rapid hydrogel formation. The choice of crosslinking method depends on the desired properties and applications of the hydrogel. By providing a biomimetic environment, hydrogels facilitate cell growth and differentiation, support tissue formation, and aid in the regeneration of damaged or diseased tissues while delivering therapeutic agents. This review focuses on the critical advancements in processing routes for hydrogel development, summarizing the characterization and application of hydrogels. It also details key applications, including wound healing and cartilage and bone regeneration, as well as the challenges and future perspectives in the field.
Numerical Investigation of Burial Depth Effects on Tension of Submarine Power Cables
Authors: Shen Jiayi , Liang Yingjie , Hong Huabin , Chen Jiawang
Published: November 2024

To protect submarine power cables from damage caused by anchoring and fishing, submarine power cables in shallow water areas are buried to a certain depth through a cable laying machine. However, limited attention has been paid to studying the stress behavior of submarine power cables while considering the effects of burial depth. In this research, static and dynamic analyses are carried out using three-dimensional numerical models performed by the OrcaFlex v11.0 to investigate the effects of burial depths on cable tension during the cable installation under various conditions. Numerical simulation results show that the peak tension of the submarine power cable increases linearly with the increase in burial depth. In addition, the burial depth can also change the tension state at the endpoint of the submarine power cable. The endpoint of the cable is in a compressed state when h < 2 m and the cable turns into a tensile state when h ≥ 2 m. Finally, genetic programming (GP) is used to analyze numerical simulation results to propose a prediction model that can be used to estimate the peak tension of the submarine power cable during cable installation under various burial depths in shallow sea areas. It should be noted that the proposed GP model is based on the analyses of numerical results; therefore, the GP model is open for further improvements as more experimental data become available.
Research on the Give-Way Ships Determination Based on Field Theory
Authors: Zhang Yunfeng , Shen Yong , Xie Zhexue , Liu Yihua
Published: November 2024

The Convention on the International Regulations for Preventing Collisions at Sea, 1972 (COLREGs) stipulates ships’ obligations when encountering each other. However, human action remains a primary cause of collision accidents. In the complex environment of mixed navigation involving MASS and manned ships, the applicability of the COLREGs for determining the give-way ship has faced certain challenges. Therefore, this study proposes a model for determining the give-way ship, combining ship characteristics and using an asymmetric Gaussian function to construct the potential field of stand-on ships from the perspective of give-way ships. It constructs the cost function based on field theory to determine the respective avoidance costs for both ships in a crossing situation, with the ship incurring the lowest cost selected as the give-way ship, followed by a case study to validate the model. The research is dedicated to coordinating avoidance action objectively, effectively reducing maritime collisions, and providing exploratory guidance for collision avoidance decision-making in future mixed navigation environments.
Prediction of Thin Shoal Reservoirs Under Reef Controlled by Isochronous Stratigraphic Framework
Authors: Xu Shoucheng , Hu Xiuquan , Shi Zejin , Zhang Chao , Mao Jintao , Wang Boqiang
Published: November 2024

Despite the great success in the global exploration and development of reef reservoirs, research on bioclastic shoals under reefs is still in its infancy. Bioclastic shoal reservoirs are very thin, with multiple vertical levels and fast lateral changes, which makes accurate prediction of the reservoir’s location much tougher. To further implement the reservoir distribution, under the guidance of sequence stratigraphy, the prediction of thin shoals under the control of an isochronous stratigraphic framework was established. Using the combination of spectrum shaping and F-X domain noise suppression techniques and utilizing the signal-to-noise ratio spectrum set as the reference, logging curve as supervision, and well seismic calibration and isochronal amplitude slicing as quality control, the seismic frequency band was extended, and the seismic data resolution and signal-to-noise ratio were improved. After frequency extension, the global optimal seismic automatic interpretation technique was used to construct an isochronal stratigraphic framework model. Through waveform facies-controlled inversion and waveform facies-controlled simulation techniques, the elastic properties of the shoal reservoir were obtained, from which the planar distribution of the reservoir was accurately predicted. The above methods were applied to the prediction of the bioclastic shoal reservoir in the lower submember of the Changxing formation in the Yuanba gas field (China). The plane distribution of bioclastic shoal in the first and second levels was identified, which provides a guideline for the prediction of thin shoal reservoirs.
A Numerical Investigation on the Aeroacoustic Noise Emission from Offshore Wind Turbine Wake Interference
Authors: Yan Yan , Xue Lei , Wang Jundong , Yang Zhichao , Xue Yu
Published: November 2024

Offshore wind turbine (WT) wake interference will reduce power generation and increase the fatigue loads of downstream WTs. Wake interference detection based on aeroacoustic noise is believed to solve these challenges in offshore wind farms. However, aeroacoustic noise is closely related to the aerodynamics around WT blades, and the acoustic detection method requires the mastery of noise emission characteristics. In this paper, FAST.Farm, combined with the acoustic model in OpenFAST, is utilized to investigate the acoustic noise emission characteristics from two 3.4 MW-130 WTs with wake interference. Multi-microphone positions were investigated for the optimal reception selection under 8 m/s and 12 m/s wind speeds with a typical offshore atmospheric turbulence intensity of 6%. The numerical simulation results indicate that wake deficit reduces the total noise emission by about 6 dBA in the overall sound pressure level (OASPL) at 8 m/s, while wake turbulence marginally increases it and its fluctuation. There is a mutual influence between these effects, and the wake deficit effect can be 100% compensated for in the OASPL at 12 m/s. Additionally, downstream observer locations are suggested based on comparisons. These investigations provide new insights into wake interference in offshore wind farms.
Genetic and Phenotypic Characterization of Botrytis Populations from Economic and Wild Host Plants in Iran
Authors: Fekrikohan Sepideh , Sharifnabi Bahram , Javan-Nikkhah Mohammad , Pollastro Stefania , Faretra Francesco , De Miccolis Angelini Rita Milvia
Published: November 2024

Grey mould disease, caused by various Botrytis species, poses a significant threat to important plants worldwide. This study aimed to characterize Botrytis populations on strawberry and roses, economically relevant host plants, and raspberry, used as a representative of wild plants, in Iran. A total of 389 isolates were collected and analyzed based on morphological features and haplotyping using molecular markers, transposable elements (Boty and Flipper), and fungicide response. Moreover, 60 isolates were used for phylogenetic analysis based on the rpb2 gene, and 16 selected isolates from each clade were further characterized using the g3pdh, hsp60, and nep2 genes. The results revealed the presence of three distinct species, Botrytis cinerea, Botrytis sinoviticola, and Botrytis prunorum, among the sampled isolates. Additionally, this study reports for the first time the presence of B. sinoviticola on strawberry and isolates belonging to B. cinerea group S in Iran. These findings provide insights into the diversity and composition of Botrytis populations on Iranian host plants.
Construction of a Joint Newmark–Runout Model for Seismic Landslide Risk Identification: A Case Study in the Eastern Tibetan Plateau
Authors: Yang Zhihua , Wu Yuming , Guo Changbao , Mai Ximao
Published: November 2024

The key to seismic landslide risk identification resides in the accurate evaluation of seismic landslide hazards. The traditional evaluation models for seismic landslide hazard seldom consider the landslide dynamic runout process, leading to an underestimation of seismic landslide hazard. Therefore, a joint Newmark–Runout model based on landslide dynamic runout is proposed. According to the evaluation results of static seismic landslide hazard, the landslide source points can be extracted, and the landslide dynamic runout process is simulated to obtain the dynamic seismic landslide hazard. Finally, the static and dynamic seismic landslide hazards are fused to obtain an optimized seismic landslide hazard. In September 2022, a strong Ms6.8 earthquake occurred in the eastern Tibetan Plateau, triggering thousands of landslides. Taking the 2022 Luding earthquake-induced landslide as a sample, the function relationship between seismic slope displacement and landslide occurrence probability is statistically modeled, which partly improves the traditional Newmark model. The optimized seismic landslide hazard evaluation of the Luding earthquake area is conducted, and then, the seismic landslide risk identification is completed by taking roads and buildings as hazard-affected bodies. The results show that the length of the roads facing very high and high seismic landslide risks are 3.36 km and 15.66 km, respectively, and the buildings on the Moxi platform near the epicenter are less vulnerable to seismic landslides. The research findings can furnish critical scientific and technological support for swift earthquake relief operations.
Defense Mechanisms of Xylopia aromatica (Lam.) Mart. in the Dry Season in the Brazilian Savanna
Authors: Campos Felipe , Vieira Maria , Sousa Marília , Jorge Letícia , Ferreira Gisela , Marques Marcia , Boaro Carmen
Published: November 2024

Water availability and light during the dry and rainy seasons in the Cerrado may influence plants’ stomatal movement and the entry of CO2 for organic synthesis, which is the main electron drain. A lower stomatal conductance may contribute to the energy accumulated in the chloroplasts being directed towards the synthesis of compounds, which contributes to the activity of antioxidant enzymes to neutralize reactive oxygen species. Xylopia aromatica is a characteristic Cerrado species, and it is often recommended for recovering degraded areas. This study aimed to investigate the influence of the dry and rainy seasons on the metabolic adjustments of Xylopia aromatica in a portion of the Brazilian savanna in the state of São Paulo. In the rainy season, better photosynthetic performance led to greater investment in essential oil production. In the dry season, the plants may direct part of their reducing sugars to the syntheses of carotenoids and anthocyanins, which may help the antioxidant enzymes to neutralize reactive oxygen species. Carotenoids assist in the dissipation of photosystem energy, which has the potential to cause oxidative stress. During this season, lower stomatal conductance prevented excessive water loss. These results suggest the acclimatization of this species to the conditions of the Brazilian savanna.
Preparation of Purpurin–Fe2+ Complex Natural Dye and Its Printing Performance on Silk Fabrics
Authors: Huang Xiaojia , Luo Jie , Wang Xiangrong , Cheng Xianwei , Hou Xueni
Published: November 2024

In order to shorten the process of textile printing with natural dyes, develop new methods, and improve the color fastness and quality of printed products, this study presents a novel approach by synthesizing a natural complex dye through the interaction between purpurin and Fe2+ ions, resulting in a compound named purpurin–Fe2+ (P-Fe). This synthesized complex dye was meticulously characterized using state-of-the-art analytical techniques, including Fourier transform infrared spectroscopy (FT-IR), ultraviolet–visible (UV–Vis) spectrophotometry, and scanning electron microscopy energy-dispersive spectroscopy (EDS). The characterization confirmed the successful complexation of purpurin with Fe2+ ions. The prepared complex dye P-Fe was used for the printing of silk fabric. The optimized printing process involves steaming at a temperature of 100 °C for a duration of 20 min. In comparison to fabrics printed using direct dyes, the K/S values of the fabric printed with the P-Fe complex showed a significant enhancement, with all color fastness ratings achieving grade four. Furthermore, the proportion of metal elements on the white background of the printed fabric was found to be less than 0.180%, and the level of whiteness was above 50. The application of the P-Fe dye in silk fabric printing not only streamlines the printing process but also enhances the depth and speed of the printed color, effectively addressing the issue of color transfer onto a white background, which is commonly associated with natural dyes.
Low-Velocity Impact Behaviour of Titanium-Based Carbon-Fibre/Epoxy Laminate
Authors: Sun Jing , Chen Weilin , Luo Hongjie , Xie Xingfang , Zhang Jingzhou , Ding Chao
Published: November 2024

This study investigated the low-velocity impact response of titanium-based carbon-fibre/epoxy laminate (TI-CF FML). A comprehensive experimental study was carried out with impact energies ranging from 16.9 J to 91.9 J. Finite element analysis, performed using ABAQUS, was employed to elucidate the failure mechanisms of the laminate. Three distinct damage modes were identified based on the impact energy levels. The energy absorption characteristics of the TI-CF FML were analysed, revealing that maximum energy absorption is achieved and remains constant after penetration occurs. The relationship between impact force and displacement was also explored, showing that the laminate can withstand a peak force of 13.1 kN. The research on the impact resistance, damage mechanisms and energy absorption capacity of TI-CF FML provides an in-depth understanding of the impact behaviour of the laminate and its suitability for various industrial applications.
Study on Springback Behavior in Hydroforming of Micro Channels for a Metal Bipolar Plate
Authors: Su Zonghui , Xie Wenlong , Xu Yong , Li Changsheng , Xia Liangliang , Yang Baocheng , Gao Mingyu , Song Hongwu , Zhang Shihong
Published: November 2024

Bipolar plates are one of the most important components of proton exchange membrane fuel cells. With the miniaturization of bipolar plate flow channel sizes and the increasing demand for precision, springback has become a key focus of research in the bipolar plate forming process. In this paper, the hydroforming process for 316L stainless steel bipolar plates was studied, and an FEM model was built to examine the stress and strain at various locations on the longitudinal section of the plate. Modeling accuracy was validated by the comparison of experimental profile and thickness distribution. The effects of forming pressure and grain size on springback behavior are discussed. The results show that with increasing forming pressure, the springback value decreases initially, followed by an increase, but then again decreases. When the forming pressure is 80 MPa–100 MPa, the deformation of the lower element of the upper rounded corner is not uniform with more elastic regions, and the springback is positively correlated with forming pressure. The springback distribution pattern on the cross-section of the bipolar plate changes from a normal distribution to a distribution of “M” shape with increased pressure. The larger the grain size, the lower the yield strength elastic proportion, resulting in a decrease in springback of the sheet. The maximum amount of springback of the bipolar plate is 3.1 μm when the grain size is 60.7 μm. The research results provide a reference for improving the forming quality of metal bipolar plates with different flow channel shapes.
Experimental Investigation and NSGA-III Multi-Criteria Optimization of 60CrMoV18-5 Cold-Work Tool Steel Machinability Under Dry CNC Hard Turning Conditions
Authors: Fountas Nikolaos A. , Papantoniou Ioannis G. , Manolakos Dimitrios E. , Vaxevanidis Nikolaos M.
Published: November 2024

This work concerns an experimental investigation dealing with the machinability of 60CrMoV18-5 cold-work tool steel under dry CNC hard turning conditions using a CBN cutting insert. A response surface experiment based on the central composite design was set to conduct dry CNC hard-turning experiments with three different levels for cutting conditions, cutting speed Vc (m/min), feed rate f (mm/rev), and depth of cut α (mm) while selecting main cutting force and surface roughness Ra as the two machinability responses. The results were analyzed by applying analysis of variance (ANOVA). The effect of cutting conditions on main cutting force and surface roughness was studied through contour plots. Full quadratic regression models were generated to model the relationships between inputs and outputs. Finally, the NSGA-III algorithm was applied to simultaneously optimize the selected machinability parameters by providing beneficial values for determining cutting conditions. The results have shown that surface roughness is mainly affected by feed rate and cutting speed, whereas main cutting force is affected by depth of cut and feed rate.