bluuebunny/crossref_metadata_2025 huggingface dataset:
A Critical Analysis on Legal Awareness of Farm Based Agri-input Enterpreneurs on Agri Enterprises in Central Telangana Region
Authors: Srishailam B. , Jirli B. , Chowdary K. Raghavendra
Published: August 2022

Agriculture is the primary source of livelihood for about 58% of Indiaâs population. Gross Value Added byagriculture, forestry, and fishing was estimated at Rs. 19.48 lakh crore (US$ 276.37 billion) in FY20. Share ofagriculture and allied sectors in gross value added (GVA) of India at current prices stood at 20.2 % in FY20.(Source: National Statistical Office (NSO). In a contrast situation where farmersâ suicide and distress stillremain the key issues in Indiaâs Agricultural scenario, we are dreaming of achieving a doubling income forthe farming community. This gap in the income level of farmers can be bridged by adopting newer incomegenerating opportunities like establishing Farm based enterprises. Farm based enterprises are the vehiclesfor transforming rural India, these enterprises are maintained by the agripreneurs. More than half (51.42%)Agri enterprises are established units in agriculture and allied sectors established in rural areas (source:Annual report MSME 2020-21). Core thrust of research is to know about the legal awareness on Agri inputenterprises like seeds, pesticides and fertilizers and their impact on rural people. Exploratory and Ex-PostFacto research design was adopted for the study. The study was conducted in Sanga reddy district ofcentral Telangana purposively as it is having maximum number of Agri-enterprises in the state. FromSanga reddy three revenue divisions namely Zahirabad, Narayanakhed and Sanga Reddy were chosenpurposively and from each revenue division Nine blocks were selected purposively thus making up a totalof 27 blocks. From each block 7-8 respondents were interviewed and collected data with help of speciallydesigned interview schedule thus constitutes a total of 200 farm based Agri-input entrepreneurs. The findingsof the study revealed that an overwhelming (97.50%) possess medium level of legal awareness on farmbased agri inputs related information, followed by high level (2.5%) of awareness.
Ecological Changes in Ancient India
Authors: Rakesh Mr.
Published: August 2022

A few studies available have focused on climate change, migration and topographical change in ancient India, however, topographical changes, history and migration is fully interconnected to each other.Many studies based on literary and archaeology suggested that Aryans migrated to the Ganga Yamunadoab from the northwest, but scholars do not inform what route they followed and what topographicalchanged were brought by Aryans during the earlier migration? Aryan followed the course of the Yamunaand Ganga because this route was less dense forest. While, north region was surrounded by hilly regionand cold, while, south region (modern Rajasthan) was dry zone area, as well as, most of the area was notsuitable for agriculture. The studies (Basham, 1967; Romila Thapar, 2002) explained how Aryan settled inthe Ganga plain but these did not throw light over the Aryanâs migration in the humid zone. However,archaeological evidences suggested that people were already moving from the west to east; even fromsouth to north. But archaeologist failed to explain pattern of migration during 1000-1500 BCE. It seems thatenvironmental conditions were much favourable in the Ganga valley; as well as, the area was suitable forthe mixed economy.
Reassessing the Association Between Economic Growth and Environment: An Indian Case
Authors: Paul Arnob
Published: August 2022

The standard of living of people is improving as the global economy grows and develops, but economicprogress is frequently associated with environmental damage, either directly or indirectly. This paper attemptto answer the question whether GDP is granger causing CO2 emission or CO2 emission is granger causingGDP. The data for the study have been collected mainly from the secondary sources such as WB databank,research papers, government reports etc.
Exploring the Probiotic and Plant Growth Promoting Potential of Endophytic Bacteria Isolated from the Vegetable Crop Bhendi Abelmoschus esculentus
Authors: Baskaran D. , Benazir Begum S. , Jenifer Annis Christy J. , Harisma B. R. , Murugappan R.M.
Published: August 2022

Endophytes are microbes that reside within the plant tissues and facilitate the growth of the host plant. Inthe present study, occurrence of endophytic bacteria in the most common vegetable plant of India, Bhendi(Abelmoschus esculentus) was screened and analysed for its plant growth promoting (PGP) potential. Presenceof a rod shaped bacterium within the tissues of the host plant was observed. The isolate was identified asBacillus firmus by its biochemical profile. Phosphate solubilization and IAA production by the isolate at 60h of incubation was found to be 37.3 ï­g mlâ1 and 39.47 ï­g mlâ1 respectively. B. firmus was found to producetrihydroxamate type siderophore with hexadentate nature. The isolate exhibited extracellular pectinolytic,proteolytic, cellulolytic, chitinolytic enzyme activity. Further, the isolate exhibited probiotic properties suchas tolerance to acid (pH2), bile salt (2%), gastric juice, auto-aggregation potential, antibiotic resistance andthe absence of haemolytic activity. Seed biopriming and scanning electron microscope (SEM) imagingconfirmed the endophytic and plant growth promoting potential of B. firmus. The results of this studyillustrate the possible utilization of the endophytic bacterium B. firmus as a bioinoculant for plant growthpromotion.
Antimicrobial sensitivity profile of bacterial agents in chronic suppurative otitis media patients in Samawa city
Authors: Hasan Habeeb Abdali
Published: August 2022

Aim: this study was to determine bacterial profile and their antimicrobial susceptibility patterns in COSM from patients attending the Al-Husain Teaching Hospital, Samawah, Al-Muthanna. Material and Method: One hundred and Twenty patients with CSOM who presented to the Ear, Nose and Throat (ENT) department in ENT in Al-Husain Teaching Hospital, Samawah city, from June 2019 to May 2021 were prospectively studied. The discharge was either frankly purulent, muco-purulent, serous or blood stained on occasion. Detailed clinical history regarding unique identification number, name, age, sex, duration of discharge, other associated symptoms and antibiotic therapy were taken. Result: Chronic suppurative otitis media (CSOM) is the result of an initial episode of acute otitis media and is characterized by a persistent discharge from the middle ear through a tympanic perforation. It is an important cause of preventable hearing loss, particularly in the developing area study. Conclusion : The predominant OM aetiologies were Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, and Staphylococcus aureus, and they were generally susceptible to most of the antibiotics tested. Amikacin, cefepime, ciprofloxacin, and meropenem could be valuable in the empirical management of   OM.
Midline stoma via the umbilicus versus traditional diverting loop ileostomy
Authors: Farooque Mohammad , Bhatt Lauve , Desai Zeel
Published: August 2022

Diverting loop ileostomies have traditionally been fashioned in the right iliac fossa, but a novel technique of midline loop ileostomy at the umbilical port site during colorectal surgeries have been described. We aim to compare outcomes after creation and reversal of these two types of ileostomy. Using a retrospective study design, 10 consecutive patients who underwent a midline loop ileostomy were compared with 10 consecutive patients who underwent a traditional right iliac fossa stoma. Baseline characteristics, operating time, length of stay and complications for the initial operation as well as reversal of stoma operation were compared between the two groups. There was no significant difference in duration of operation or complication rate after the initial stoma formation, but length of hospital stay was longer in the traditional stoma group (10 vs 4.5 days, p = 0.04). After reversal of ileostomy, the traditional stoma group had a significantly higher perioperative complication rate compared to the midline stoma group (50 vs 0%, p = 0.03), but there was no difference in operation time or length of hospital stay.
Insole modification as a plantar pressure distributor in diabetic polyneuropathy
Authors: Respati Dimas Bagus , Pawana I Putu Alit
Published: August 2022

73 years old, male, referred from Internal Medicine department with diagnose Diabetic Polyneuropathy. The patient felt numbness at both feet since about 8 years ago and also complain about shortened walking distance because of fatigue. From the physical examination we found that has muscle weakness at left leg. From the foot examination there were decreased vibration and light touch sensation, anhidrosis, hyperkeratosis at his feet. There were also charcot arthropathy and decreased of foot fat pad. Foot pressure examination was high. The treatment goal for this patient is to wear comfortable footwear, improving walking endurance, and preventing future ulceration. The insole was needed to decrease plantar pressure during ambulation. Recommended rehabilitation treatment involves interaction between the health care team and the patient to achieve rehabilitation goals.
Coaching commitment and physical development of student-athletes from various public schools in Angeles City, Pampanga, Philippines
Authors: Lobo Joseph , Bautista Clarenz , Dimalanta Genesis , Manuel Susan
Published: August 2022

This present study aims to determine the relationship between coaching commitment and physical development of student-athletes from various public schools in Angeles City, Pampanga, Philippines. Respondents on the study are student-athletes from Senior High School Public Schools from Grade 11 and 12. Descriptive statistics such as Mean and Standard Deviation were used to describe the level of coaching commitment and physical development according to categories, and Pearson Product Moment Correlation Coefficient was used to determine the relationship between coaching commitment and physical development. The findings revealed that the level of coaching commitment is very high and the level of physical development of the respondents is highly developed. From the correlational analysis, result findings revealed that competition strategies, goal setting, physical training and condition, teamwork, technical skills, and time management were observed to have no significant relationship to caring, character, competence, confidence, and connection of the respondents. On one hand, mental preparation was observed to have a significant negative relationship on caring. Additionally, Personal Rapport/ Negative Personal Rapport were observed to have a significant positive relationship on Caring, Character and Connection.
Small check size pattern reversal visual evoked potentials (VEPs) in patients with chronic idiopathic intracranial hypertension
Authors: Khames Marwa Abd Elsalam , Alim Hanan Hosny Abdel , Elhabashy Hala Rashad , Tawfik Mona Hussein , Khames Marwa Abd Elsalam
Published: August 2022

The condition known as idiopathic intracranial hypertension is characterized by elevated intracranial pressure. The most feared complication of IIH is visual loss due to papilledema and optic nerve atrophy. Visual evoked potentials are sensitive and non-invasive method. They are considered an important objective test in investigating suspected optic nerve disease. This study aims to clarify the effect of chronic increased ICP on the optic nerve. Methods: This case-control study was performed at Beni-Suef University Hospital on 60 female patients divided into two equal groups; Group (I):  thirty female patients with chronic idiopathic hypertension and Group (II): thirty age and sex-matched healthy controls. All subjects were subjected to full neuro-ophthalmological assessment by PVEP, a lumbar puncture to measure CSF pressure, and radiological assessment using MRI. SPSS version 20 was used for the statistical analysis. Results: Right Pattern reversed VEP; for the check size 15min, the mean value of P100 latencies of chronic IIH was 109.88±10.46 and for controls was 97.35±4.23. The mean value of P100 amplitudes of chronic IIH was 11.73±5 and for controls was 11.95±3.3. Left pattern reversed VEP; for the check size 15min, the mean value of P100 latencies of chronic IIH was 111.42±11.5 and for controls was 97.13±4.32.
A study of arrhythmias and its outcome in patients with acute coronary syndrome
Authors: Chandregowda Chandregowda , Unnikrishnan Anagha , Rajan Sini
Published: August 2022

Aim: To detect arrhythmia during hospitalization of patients with acute coronary syndrome and to correlate the association between arrhythmia and patient outcome. Introduction: Acute coronary syndrome represents a Global Pandemic, and is intimidating large as the new epidemic afflicting population worldwide, especially in the Indian sub-continent. Studies have shown that arrhythmias are important predictors of poor outcome in patients with ACS and are associated with higher in-hospital mortality. So, ECG is of great value in patients with acute MI and is essential for risk stratification and selection of optimal management. Methods: A Prospective clinical study consisting of 100 patients of acute coronary syndrome were admitted in ICCU of Mandya Institute of medical Sciences, Mandya who are aged between 18 –50yrs during the period of study (January 2018 to December 2018, a period of 12 months) were taken to determine the occurrence of arrhythmia, its predictors and outcome. Patients were assessed with Characteristic history, ECG criteria and Elevated cardiac enzymes. Results: A total of 100 patients are taken, on admission to the intensive coronary care unit at Mandya Institute of Medical Sciences, Mandya. They included 56 males and 44 females (52%).
Decoding the changing etiology and management protocols of intestinal obstruction: A prospective clinical study
Authors: Rao G. Nagabhushana , S. R. Nagendrababu S. R. Nagendrababu , Anjali Gorle sri , Sarvani Gaddipati , Raju D. S. S. K.
Published: August 2022

Background: Obstruction of the bowel is one of the most common problems intra-abdominally seen by surgeons in their practice. The reasons associated with these obstructions are biochemical disturbances correlated to adhesion, neoplasms, and/or hernia. Obstruction of either the large intestine or small intestine remains the major cause of mortality and morbidity. Methods: The study included a total of 48 subjects having intestinal obstruction within the age range of 18-87 years. The diagnosis was made based on hematologic parameters, radiologic parameters, physical examination, and clinical history of the subjects. Results: Most common complication seen in 14.58% (n=7) study subjects, followed by the surgical site infection seen in 10.41% (n=5) study subjects. Respiratory tract infection was seen in 8.33% (n=4) of study subjects. The least common reported complication was faecal fistula seen in 4.16% (n=2) of study subjects. On assessing the mortality rate of the present study, it was seen that 12.5% (n=6) subjects died following surgical management of acute abdominal distension, whereas, 87.5% (n=42) subjects survived and were cured Conclusion: The present study concludes that the most common reason behind intestinal obstruction was postoperative adhesion which can be accurately diagnosed with operative, radiological, and clinical assessment.
Spatial variation of the change in the cultivation of horticultural crops in Al-Qaim district for the period (2000 - 2020)
Authors: Alrawi Abdalkader Maan Ismail , Alsalmani Ahmed Hilaal Hmood
Published: August 2022

The research concluded by studying and analyzing the areas planted with horticultural crops in Al-Qaim district at the level of agricultural districts for two different periods of time in order to analyze the amount of variation and change in the area planted with these crops, whether positive or negative. Therefore, the year (2000) was taken as the base year and (2020) as the target year, to determine the levels of change between them. It was found through the tables and maps of the geographical distribution that there was a significant change in the areas planted with these crops during the study period and at the level of one province, and at a rate of decline in those areas Its amount reached (507) dunums, and the number of trees decreased by (63,178), which gives a serious indication of the decline in agricultural areas and crops in general, whose effects will be reflected on the residents of the region and their economic levels.
GOVERNMENT ENCOURAGING SOCIAL AND ECONOMIC DEVELOPMENT THROUGH BY MIXED CAPITAL BANK: STIMULATING SOCIAL ENTREPRENEURSHIP
Authors: Machado Junior Celso , Aurélio Rony Roberto , Ribeiro Daielly Melina Nassif Mantovani , Viana Adriana Backx Noronha
Published: August 2022

The analysis of government actions promoted by the state is present in several surveys. However, there are few contributions that analyze the social benefits of activities developed by public institutions of mixed capital, that reconcile social and economic aspects. This research aims to analyze the social effectiveness of a corporate social responsibility action of a mixed capital financial institution. Documentary research was developed in 11,377 projects, combined with the application of a questionnaire. It was identified that corporate social responsibility actions should prioritize social impact, positioning financial return and reputation as secondary aspects, in order to guarantee the social intentions of the institution promoting the activity, otherwise it will only be addressed how a investment for marketing strategies. Projects that seek to develop economic activities in underserved locations provide significant and lasting social impacts. A significant benefit of Corporate Social Responsibility is to empower people to be social entrepreneurs, and to encourage the dissemination of the culture of social entrepreneurship, thus enhancing the occurrence of more projects that serve the less favored economic classes.
REGIONAL MANAGEMENT AND DEVELOPMENT WITH FREE MULTI-TEMPORAL IMAGES: THE CASE OF HYDROELECTRIC POWER INSPECTION
Authors: Orlandi Alex Gois , De Carvalho Junior Osmar Abí­lio , Mendonça Rodrigo Cesar Neves , Guimarães Renato Fontes , Gomes Roberto Arnaldo Trancoso
Published: August 2022

The present research aims to analyze the case of the use of free satellite images in the inspection of works of hydroelectric plants and to propose its use in the management of regional development. The study performs a multitemporal analysis of CBERS-4 and Sentinel-2 data to assist the inspection process of the National Electric Energy Agency and proposes a reflection on this technology as a free tool for regional development management. The methodology observed inspection reports, images with different temporal and spatial resolutions and the ability of the images to integrate the inspection process of electricity generation in Brazil through photointerpretation. The results demonstrate that the processing of free images optimizes the human and financial resources, being able to verify three of the five stages of works supervised by the Agency to guarantee the schedule of implantation of the hydroelectric plants in any region of the country with the same capacity of use. In addition, remote sensing data are presented as an excellent tool in the management of regional development where the plants are implemented or other related management and monitoring applications, as they provide greater transparency and efficiency to the control over large areas, such as that exercised by the Agency, strengthening accountability and considerably reducing one of the most sensitive aspects to the regulatory body and the management of large areas: information asymmetry.
Development and Validation of An Evaluation Scale for Audiovisual Production for Health Interventions - ZIKAMOB
Authors: da Costa Emily Galdino , Fernandes Izabelly Dutra , Albino Victor Alves , Smania-Marques Roberta , Olinda Ricardo , da Silva Leandro Fernandes , de Lima Adrielly Karoliny , Barbosa Lourenço Eli Mateus , Galisa Steffany Sales , Albuquerque Emanuelly Oliveira Muniz e , Smith Matt , Traxler John , Santos Silvana
Published: August 2022

According to the World Health Organization, intervention actions and Health Education achieve better performance when based on Behavior Change Theories associated with new technologies. This work aimed to build and validate an Audiovisual Production Assessment Scale (APAS) for use in educational interventions. One hundred videos of up to 90 seconds in length, produced by high school students from Northeast Brazil, were analyzed. The APAS contains twenty statements, grouped into five sections, some of which are based on the Social Cognitive Theory (observational learning; facilitators) and others, such as the halo effect and cognitive comfort, were proposed by Daniel Kahneman. It was found that, of the twenty statements, 15 of them had no significant difference between different evaluators; having obtained a value of 0.941 for Cronbach's Alpha, showing excellent internal reliability of the APAS. On average, 22 (33.8%) videos received a score greater than 60 points, indicating that they have the potential to significantly contribute to population behavior change in relation to the prevention of mosquito-borne arboviruses; 28 (41.3%) contribute satisfactorily; 15 (22.9%), partially and from one to two videos were scored with values lower than 19 points. Altogether, 12% of the videos received maximum scores in relation to the total score and subjective score. The APAS is, therefore, an example of an effective tool for assessing audiovisual content that can be used in educational interventions in health, with good internal reliability. The scale allows evaluating any content, classifying the production into categories that reveal its potential to promote behavior change.
Footprint of Nitric oxide in induced systemic resistance
Authors: Acharya R , Patra P , Chakraborty N , Gupta N S , Acharya K
Published: August 2022

Nitric oxide (NO) is a potent signaling molecule with diverse physiological functions in plants. Several rhizobacterial strains may have capacity to induce systemic resistance in (ISR) plants but how far the biochemical mechanisms in which No participates in this signaling pathway is still an open question. The present study have shown in Pseudomonas aeruginosa WS-1 mediated ISR inducing system in Catharanthus roseus induces defense enzyme and phenolics and also showed a two fold increase in NO production when challenge with Alternaria alternata. Furthermore, NO donor treatment in the host produced same defense molecules in a comparable manner. From those observations it is suggested that NO might have possible signaling role in ISR during crosstalk between the ISR inducing agent and pathogen within the host system.
Dark -Field Microscopic Analysis on the Blood of 1,006 Symptomatic Persons After Anti-COVID mRNA Injections from Pfizer/BioNtech or Moderna
Authors: Benzi Cipelli Riccardo , Giovannini Franco , Pisano Giampaolo
Published: August 2022

The use of dark-field microscopic analysis of fresh peripheral blood on a slide was once widespread in medicine, allowing a first and immediate assessment of the state of health of the corpuscular components of the blood. In the present study we analyzed with a dark-field optical microscope the peripheral blood drop from 1,006 symptomatic subjects after inoculation with an mRNA injection (Pfizer/BioNTech or Moderna), starting from March 2021. There were 948 subjects (94% of the total sample) whose blood showed aggregation of erythrocytes and the presence of particles of various shapes and sizes of unclear origin one month after the mRNA inoculation. In 12 subjects, blood was examined with the same method before vaccination, showing a perfectly normal hematological distribution. The alterations found after the inoculation of the mRNA injections further reinforce the suspicion that the modifications were due to the so-called “vaccines” themselves. We report 4 clinical cases, chosen as representative of the entire case series. Further studies are needed to define the exact nature of the particles found in the blood and to identify possible solutions to the problems they are evidently causing.
Effectiveness of Football Learning Through the Playing K Approach to Motor Ability for Riau Sports High School Students
Authors: Fernando Ricky , Zulrafli Zulrafli , Sasmarianto Sasmarianto
Published: August 2022

Football learning has a complex movement that is very skillful (Sartono et al., 2020). The skills taught are not just playing skills, but more on the actual application of the game in football on the field. The influence of physical education subject teachers also plays a very important role in the success of football learning (Effendi & Rhamadhansyah, 2017). It must also provide changes to all components that exist in the structure of football movement. In addition, the keywords of soccer learning have three important aspects in learning, namely cognitive, affective, and psychomotor (Nururi & Sulaiman, 2015; Taufik & Gaos, 2019). This study aims to improve the motor ability of Riau Sports High School students in learning football. The method used in this research is the experimental method. The results showed that the play approach was better than the technical approach. So it can be concluded that the play approach is considered more effective and can be applied inlearning football in schools.
Entre cantoras, matronas e poetas: significando o blues
Authors: Rocha Alexandre Eleutério , Pereira Flávio Santos , Cayón Durán Luis Abraham
Published: August 2022

Este artigo aborda o blues. Tem por objetivo a análise de aspectos marcantes da estética e linguagem desse secular gênero musical afro-americano: a agridoce ambiguidade discursiva, emotiva e comportamental, linguagem culturalmente codificada cujo fio condutor é o recorte no(s) conceito(s) de significação. Foram escolhidos eixos analíticos centrados na tétrade reflexiva estrutura, processo, função e significado. O caminho que possibilita a análise dessa manifestação artística é uma revisão bibliográfica que se vale de arcabouço teórico-metodológico da Musicologia Cultural, Etnomusicologia e Semiologia Musical. Recorre-se também a outros suportes teóricos de áreas como Antropologia, História e Estudos Literários. É abordada igualmente a estreita relação da estética, linguagem e discurso do blues - semântica e sintaxe - com a construção sócio-histórica e cultural da identidade étnica do afro-americano.
Resenha: Piano.Pérolas: quem brinca já chegou!
Authors: Uzun Helena , Malagutti Vania
Published: August 2022

O livro “Piano.Pérolas: Quem brinca já chegou!”, de autoria das professoras, pianistas e compositoras Carla Reis e Liliana Botelho, foi lançado em 2019. O material é composto por 18 obras autorais para iniciantes, pautadas no ensino por imitação, de modo que a leitura musical é considerada como um dos aspectos a serem abordados no processo do ensino de um instrumento musical. Nesta direção, o livro traz subsídios pedagógico-musicais que contemplam, por exemplo, a topografia do teclado, a aquisição da técnica, a exploração sonora, a criatividade e a compreensão de elementos de estruturação musical. A proposta traz peças em solo e duos e disponibiliza a gravação das mesmas em um canal do Youtube. O material se constitui em uma possibilidade prática e viável que considera as demandas reais do ensino do piano no século XXI, oferecendo um caminho que contempla a performance e a criação musical.
Uma teoria formal de funções tonais generalizadas
Authors: Defensor Felipe
Published: August 2022

Tradução do seminal artigo A Formal Theory of Generalized Tonal Functions de David Lewin, publicado em 1982, que prefigura algumas ideias mais tarde desenvolvidas em Generalized Musical Intervals and Transformations (LEWIN, 1987) e que dariam origem à teoria transformacional e à teoria neorriemaniana. No artigo é construída, por meio de formalização matemática, uma estrutura denominada Sistema Riemann, que generaliza certos conceitos pertinentes à teoria da música tonal, como o de função harmônica e o de função melódica. São também conceituadas transformações que relacionam Sistemas Riemann entre si, abarcando e estendendo o escopo das transformações riemanianas tradicionais. Tais transformações podem ser interpretadas como correspondentes a outras noções teóricas familiares, como a transposição, a inversão, o “se tomar a relativa”, o “se tomar a mediante”, entre outras. Algumas pequenas análises de excertos de Wagner, Brahms, Stravinsky e Beethoven são elaboradas a título de mostrar a pertinência da teoria desenvolvida para a análise musical.
A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regeneration
Authors: Brandão Ana S , Borbinha Jorge , Pereira Telmo , Brito Patrícia H , Lourenço Raquel , Bensimon-Brito Anabela , Jacinto Antonio
Published: August 2022

Regeneration depends on the ability of mature cells at the injury site to respond to injury, generating tissue-specific progenitors that incorporate the blastema and proliferate to reconstitute the original organ architecture. The metabolic microenvironment has been tightly connected to cell function and identity during development and tumorigenesis. Yet, the link between metabolism and cell identity at the mechanistic level in a regenerative context remains unclear. The adult zebrafish caudal fin, and bone cells specifically, have been crucial for the understanding of mature cell contribution to tissue regeneration. Here, we use this model to explore the relevance of glucose metabolism for the cell fate transitions preceding new osteoblast formation and blastema assembly. We show that injury triggers a modulation in the metabolic profile at early stages of regeneration to enhance glycolysis at the expense of mitochondrial oxidation. This metabolic adaptation mediates transcriptional changes that make mature osteoblast amenable to be reprogramed into pre-osteoblasts and induces cell cycle re-entry and progression. Manipulation of the metabolic profile led to severe reduction of the pre-osteoblast pool, diminishing their capacity to generate new osteoblasts, and to a complete abrogation of blastema formation. Overall, our data indicate that metabolic alterations have a powerful instructive role in regulating genetic programs that dictate fate decisions and stimulate proliferation, thereby providing a deeper understanding on the mechanisms regulating blastema formation and bone regeneration.
Transcriptional heterogeneity and cell cycle regulation as central determinants of Primitive Endoderm priming
Authors: Perera Marta , Nissen Silas Boye , Proks Martin , Pozzi Sara , Monteiro Rita S , Trusina Ala , Brickman Joshua M
Published: August 2022

During embryonic development cells acquire identity as they proliferate, implying that an intrinsic facet of cell fate choice requires coupling lineage decisions to cell division. How is the cell cycle regulated to promote or suppress heterogeneity and differentiation? We explore this question combining time lapse imaging with single-cell RNA-seq in the contexts of self-renewal, priming, and differentiation of mouse embryonic stem cells (ESCs) towards the Primitive Endoderm (PrE) lineage. Since ESCs are derived from the inner cell mass (ICM) of the mammalian blastocyst, ESCs in standard culture conditions are transcriptionally heterogeneous containing dynamically interconverting subfractions primed for either of the two ICM lineages, Epiblast and PrE. Here, we find that differential regulation of cell cycle can tip the balance between these primed populations, such that naïve ESC culture promotes Epiblast-like expansion and PrE differentiation stimulates the selective survival and proliferation of PrE-primed cells. In endoderm differentiation, this change is accompanied by a counter-intuitive increase in G1 length, also observed in vivo. While fibroblast growth factor/extracellular signal-regulated kinase (FGF/ERK) signalling is a key regulator of ESC differentiation and PrE specification, we find it is not just responsible for ESCs heterogeneity, but also the inheritance of similar cell cycles between sisters and cousins. Taken together, our results indicate a tight relationship between transcriptional heterogeneity and cell cycle regulation in lineage specification, with primed cell populations providing a pool of flexible cell types that can be expanded in a lineage-specific fashion while allowing plasticity during early determination.
TRPV1 drugs alter core body temperature via central projections of primary afferent sensory neurons
Authors: Yue Wendy Wing Sze , Yuan Lin , Braz Joao M , Basbaum Allan I , Julius David
Published: August 2022

TRPV1, a capsaicin- and heat-activated ion channel, is expressed by peripheral nociceptors and has been implicated in various inflammatory and neuropathic pain conditions. Although pharmacological modulation of TRPV1 has attracted therapeutic interest, many TRPV1 agonists and antagonists produce thermomodulatory side effects in animal models and human clinical trials, limiting their utility. These on-target effects may result from the perturbation of TRPV1 receptors on nociceptors, which transduce signals to central thermoregulatory circuits and release proinflammatory factors from their peripheral terminals, most notably the potent vasodilative neuropeptide, calcitonin gene-related peptide (CGRP). Alternatively, these body temperature effects may originate from the modulation of TRPV1 on vascular smooth muscle cells (vSMCs), where channel activation promotes arteriole constriction. Here, we ask which of these pathways is most responsible for the body temperature perturbations elicited by TRPV1 drugs in vivo. We address this question by selectively eliminating TRPV1 expression in sensory neurons or vSMCs and show that only the former abrogates agonist-induced hypothermia and antagonist-induced hyperthermia. Furthermore, lesioning the central projections of TRPV1-positive sensory nerve fibers also abrogates drug-mediated thermomodulation, whereas eliminating CGRP has no effect. Thus, TRPV1 drugs alter core body temperature by modulating sensory input to the central nervous system, rather than through peripheral actions on the vasculature. These findings suggest how mechanistically distinct TRPV1 antagonists may diminish inflammatory pain without affecting core body temperature.
Neighborhood-based inference and restricted Boltzmann machine for microbe and drug associations prediction
Authors: Cheng Xiaolong , Qu Jia , Song Shuangbao , Bian Zekang
Published: August 2022

Background

Efficient identification of microbe-drug associations is critical for drug development and solving problem of antimicrobial resistance. Traditional wet-lab method requires a lot of money and labor in identifying potential microbe-drug associations. With development of machine learning and publication of large amounts of biological data, computational methods become feasible.

Methods

In this article, we proposed a computational model of neighborhood-based inference (NI) and restricted Boltzmann machine (RBM) to predict potential microbe-drug association (NIRBMMDA) by using integrated microbe similarity, integrated drug similarity and known microbe-drug associations. First, NI was used to obtain a score matrix of potential microbe-drug associations by using different thresholds to find similar neighbors for drug or microbe. Second, RBM was employed to obtain another score matrix of potential microbe-drug associations based on contrastive divergence algorithm and sigmoid function. Because generalization ability of individual method is poor, we used an ensemble learning to integrate two score matrices for predicting potential microbe-drug associations more accurately. In particular, NI can fully utilize similar (neighbor) information of drug or microbe and RBM can learn potential probability distribution hid in known microbe-drug associations. Moreover, ensemble learning was used to integrate individual predictor for obtaining a stronger predictor.

Results

In global leave-one-out cross validation (LOOCV), NIRBMMDA gained the area under the receiver operating characteristics curve (AUC) of 0.8666, 0.9413 and 0.9557 for datasets of DrugVirus, MDAD and aBiofilm, respectively. In local LOOCV, AUCs of 0.8512, 0.9204 and 0.9414 were obtained for NIRBMMDA based on datasets of DrugVirus, MDAD and aBiofilm, respectively. For five-fold cross validation, NIRBMMDA acquired AUC and standard deviation of 0.8569 ± −0.0027, 0.9248 ± −0.0014 and 0.9369 ± −0.0020 on the basis of datasets of DrugVirus, MDAD and aBiofilm, respectively. Moreover, case study for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) showed that 13 out of the top 20 predicted drugs were verified by searching literature. The other two case studies indicated that 17 and 17 out of the top 20 predicted microbes for the drug of ciprofloxacin and minocycline were confirmed by identifying published literature, respectively.
Flotillin-1 promotes EMT of gastric cancer via stabilizing Snail
Authors: Huang Ying , Guo Yun , Xu Yi , Liu Fei , Dai Suli
Published: August 2022

Gastric cancer is one of the most common malignancies worldwide and has been identified as the third leading cause of cancer-related mortality. Flotillin-1 is a lipid raft-associated scaffolding protein and plays an important role in the progression and development of several malignant carcinomas. Flotillin-1 is involved in epithelial-mesenchymal transition (EMT) process of several solid tumors to promote metastasis. However, the detailed characteristics and mechanisms of Flotillin-1 in gastric cancer have rarely been investigated. In this study, we found Flotillin-1 upregulated in gastric cancer, and the high expression of Flotillin-1 correlated with a worse prognosis. The migration and invasion ability of gastric cancer cells was upregulated by overexpressing Flotillin-1. Knockdown of Flotillin-1 inhibits gastric cancer cells metastasis. Flotillin-1 is a key regulator of EMT process and promotes gastric cancer cells metastasis through inducing EMT. Flotillin-1 may interact with a deubiquitinase to inhibit the ubiquitination of Snail in gastric cancer cells to promote EMT process. Our study provides a rationale and potential target for the treatment of gastric cancer.
Gênese social do editor e as novas condições de produção do livro.
Authors: De Sousa Antonio Paulino
Published: August 2022

Este artigo relaciona o problema da edição tradicional com as novas tendências no campo editorial e busca estabelecer as condições sociais de produção do livro. O editor é um mediador entre o autor e o seu público. Esta relação dialética entre editor e autor é importante para a compreensão do produto final que é o livro. O editor exerce uma função crítica determinante no processo de construção, circulação e atribuição de novos significados. Por essa razão fala-se em termos de escrita editorial, cuja atividade está sendo questionada pelas novas estratégias de leitura na Web. O problema essencial é a filtragem na Web. O gesto editorial continua sendo indispensável para a legitimação e validação científica dos discursos. Palavras-chave: Poder. Editor. Campo Editorial. Enunciação Editorial, Web. SOCIAL GENESIS OF THE PUBLISHER AND THE NEW CONDITIONS OF BOOK PRODUCTION Antonio Paulino de Sousa This article links the problem of traditional publishing with the new trends in the publishing sector and has the goal of establishing the social conditions for book production. The publisher is an intermediary between the author and his or her public. This dialectic relation between publisher and author is important to understand the book as a final product. The publisher has a critical role which is a determinant in the process of construction, circulation and attribution of new meanings. For this reason, we speak in terms of editorial writing, whose activity is being questioned by new reading strategies on the Web. The main problem is filtering on the Web. The editorial action remains essential for the legitimization and scientific validation of the discourses. Keywords: Power. Publisher. Editorial field. Editorial enunciation, Web. LA GENÈSE SOCIALE DE L’ÉDITEUR ET LES NOUVELLES CONDITIONS DE PRODUCTION DU LIVRE Antonio Paulino de Sousa Cet article fait le lien entre le problème de l’édition traditionnelle accompagnée des nouvelles tendances dans le champ de l’édition et cherche à établir les conditions sociales de production du livre. L’éditeur est un médiateur entre l’auteur et son public. Cette relation dialectique entre l’éditeur et l’auteur est importante pour bien comprendre ce produit final qui est le livre. L’éditeur exerce une fonction critique déterminante dans le processus de construction, de circulation et d’attribution de nouveaux sens. C’est pour cette raison que l’on parle en termes d’écriture éditoriale dont l’activité est remise en question par les nouvelles stratégies de lecture sur la Toile (Web). Le problème essentiel est de savoir filtrer sur la Toile (Web). Le geste éditorial continue à être indispensable pour légitimer et valider le côté scientifique des discours. Mots-clés: Pouvoir. Éditeur. Champ Editorial. Énonciation Éditorial, Toile (Web). Publicação Online do Caderno CRH no Scielo: http://www.scielo.br/ccrh  Publicação Online do Caderno CRH: http://www.cadernocrh.ufba.br
FEATURES OF THE PRODUCTION OF DOUGH SEMI-FINISHED PRODUCT PRODUCED USING LENTIL FLOUR
Authors: TERENTIEV S. , GRUNINA O. , PONOMAREVA L.
Published: August 2022

Bread consumption has a stable increase in the territory of Russia and in particular in the Ulyanovsk and Samara regions. Bread, as a fairly low-priced product, is in high demand among consumers, but this product is not biologically saturated with useful substances, therefore, in modern production, a number of techniques are used to increase the nutritional and biological value of these types of products. In our work, one of these methods will be considered - the introduction of lentil flour into dough preparations. The problem is that the state policy regarding import substitution, aimed at replacing food additives produced abroad, necessitates the use of food additives or raw materials of natural origin produced in the territory of the Russian Federation, and the lack of development of regulatory and technological documentation in this direction is a significant problem for public enterprises. nutrition. Purpose - to carry out the development of a recipe for a test semi-finished product produced with the addition of lentil flour, as a product with a preventive purpose Results: based on the results of the study, a recipe for a test semi-finished product was developed, produced with the addition of lentil flour, as a product with a preventive purpose.
TAPE – to není páska s již hotovým záznamem, ale nový časopis pro filosofii a teologii výchovy
Authors: Dlouhá Jana
Published: August 2022

Byl založen elektronický časopis s volně přístupným obsahem – TAPE. Má být společnou platformou pro teology, filozofy a pedagogy, i pro všechny další profesionály, kteří se ptají po smyslu lidského života, zajímají se o sebevzdělávání a výchovu, a chtějí přispět k etice a rozvoji humanity.
Measuring the Saudi Society's Awareness of Verbal Violence's Contribution to Low Self-Esteem, Feelings of Psychological Loneliness and Depression in Children
Authors: Barakati Shahd Ibrahim Badr
Published: August 2022

This study sought to measure the Saudi society's awareness of the impact of verbal violence on low self-esteem, feelings of psychological loneliness and depression among children , identify the forms of verbal and emotional violence practiced against the child, the effects resulting from the phenomenon of verbal violence against the child, measure the role of the media and the educational and counseling community institutions in reducing the phenomenon of verbal violence against the child, and determine the relationship of the number of family members, the level of parental education, and the social status to verbal violence against the child. The study released on the descriptive analytical approach, which depends on collecting data from the study sample using the questionnaire prepared for the purposes of this study, and studying and analyzing the sample responses. The study included a random sample of (88) fathers and mothers from the western, southern, central, northern and eastern regions of the Kingdom of Saudi Arabia. The researcher formulated a questionnaire of (23) items to measure Saudi society's awareness of the impact of verbal violence on low self-esteem, feelings of psychological loneliness and depression among adolescents. The questionnaire dealt with four
topics as follows:1. The first axis: the form of verbal and emotional violence against the child: (5) paragraph .2. The second axis: knowledge of the effects resulting from the phenomenon of verbal violence against the child (8) paragraphs .3. The third axis: the role of the media and the awareness and counseling community institutions in reducing the phenomenon of verbal violence against children (5) paragraphs .4. The fourth axis: the relationship of the number of family members to verbal violence against the child (5) paragraphs.The study revealed the forms of verbal violence practiced against the child, and the results of the questionnaire showed an expressive percentage indicating the awareness of the respondents of the effects resulting from the phenomenon of verbal violence against the child and the presence of a relative need to Strengthen the role of the media and educational and counseling community institutions in reducing the phenomenon of verbal violence against the child. The results of the study also revealed a relationship between the increased number of family members and the prevalence of verbal violence against children. The study recommended, among a set of recommendations, to allocate television programs aimed at introducing the family.
Production of English Consonants: Bilabial Voiced /P/ and the Voiced Labiodental Fricative /V/ by Saudi ESL/EFL Students
Authors: Alharbi Samar
Published: August 2022

The language systems and the production of letter sounds seem to be different among people who speak different languages. Non-native speakers of English have some variations in language production and sound when they speak English due to the differences between the native language and the target language. This paper explores the issues related to the absence of the bilabial stop /p/ and the voiced labiodental fricative /v/ in Arabic speakers. It also presents some recommendations to overcome pronunciation problems.