bluuebunny/crossref_metadata_2025 huggingface dataset:
The Performance of Quantiferon-TB Gold in-Tube (QFT-IT) Test Compared to Tuberculin Skin Test (TST) in Detecting Latent Tuberculosis Infection (LTBI) in the Presence of HIV Coinfection in a High TB-Burden Area with BCG-Vaccinated Population
Authors: James Pratibha Malini , Ganaie Feroze Ahmed , Kadahalli Ravikumar Lingegowda
Published: March 2013

Objectives:

To compare quantiferon-TB gold “in tube” (QFT-IT) with the conventional tuberculin skin test (TST) for the diagnosis of latent tuberculosis infection (LTBI) in HIV-infected adults in a setting highly endemic for tuberculosis with BCG vaccinated population in Bangalore, Karnataka, India.

Patients and Methods:

The study population (100 BCG-vaccinated adults) was divided into 4 groups: HIV patients with TB patient contact, HIV patients with past history of TB, TB patients (positive control), and healthy volunteers (negative control).

Results:

Overall agreement between TST and QFT-IT was 52.4% (κ = 0.22). Increasing the TST cutoff value from 5 mm to 10 mm among HIV-positive groups resulted in better agreement 62.5% in Group 1 and 81.2% in Group 2.

Discussion:

In a setting with high TB prevalence, the TST gives increased false positives in HIV-positive groups if 5 mm is used as the cutoff value. This could be minimized by increasing the cutoff to 10 mm.

Conclusion:

The 2-step approach (initial testing of all cases with TST and confirmation of only those positive in TST by testing with QFT-IT) will be economical and help in treating LTBI cases in lower middle income countries like India.
SOX4 is a direct target gene of FRA-2 and induces expression of HDAC8 in adult T-cell leukemia/lymphoma
Authors: Higuchi Tomonori , Nakayama Takashi , Arao Tokuzo , Nishio Kazuto , Yoshie Osamu
Published: March 2013

Key Points

SOX4 is consistently expressed in ATL, is involved in ATL cell growth, and induces genes such as GCRK, NAP1, and HDAC8 in ATL. FRA-2/JUND and SOX4 form an important oncogenic cascade in ATL, leading to upregulation of genes such as HDAC8.
Autologous/reduced-intensity allogeneic stem cell transplantation vs autologous transplantation in multiple myeloma: long-term results of the EBMT-NMAM2000 study
Authors: Gahrton Gösta , Iacobelli Simona , Björkstrand Bo , Hegenbart Ute , Gruber Astrid , Greinix Hildegard , Volin Liisa , Narni Franco , Carella Angelo Michele , Beksac Meral , Bosi Alberto , Milone Giuseppe , Corradini Paolo , Schönland Stefan , Friberg Kristina , van Biezen Anja , Goldschmidt Hartmut , de Witte Theo , Morris Curly , Niederwieser Dietger , Garderet Laurent , Kröger Nicolaus
Published: March 2013

Key Points

Tandem autologous/reduced-intensity allogeneic transplantation is superior to autologous transplantation alone in multiple myeloma.
A note on the "Equation missing" -zeta-type function with weight α
Authors: Cetin Elif , Acikgoz Mehmet , Cangul Ismail Naci , Araci Serkan
Published: March 2013

The objective of this paper is to derive the symmetric property of an

(
h
,
q
)

-zeta function with weight α. By using this property, we give some interesting identities for

(
h
,
q
)

-Genocchi polynomials with weight α. As a result, our applications possess a number of interesting properties which we state in this paper.

MSC:11S80, 11B68.
Characterization of expressed sequence tags from developing fibers of Gossypium barbadenseand evaluation of insertion-deletion variation in tetraploid cultivated cotton species
Authors: Lv Yuanda , Zhao Liang , Xu Xiaoyang , Wang Lei , Wang Cheng , Zhang Tianzhen , Guo Wangzhen
Published: March 2013

Background

Cotton is the leading fiber crop worldwide. Gossypium barbadense is an important species of cotton because of its extra-long staple fibers with superior luster and silkiness. However, a systematic analysis and utilization of cDNA sequences from G. barbadense fiber development remains understudied.

Results

A total of 21,079 high quality sequences were generated from two non-normalized cDNA libraries prepared by using a mixture of G. barbadense Hai7124 fibers and ovules. After assembly processing, a set of 8,653 unigenes were obtained. Of those, 7,786 were matched to known proteins and 7,316 were assigned to functional categories. The molecular functions of these unigenes were mostly related to binding and catalytic activity, and carbohydrate, amino acid, and energy metabolisms were major contributors among the subsets of metabolism. Sequences comparison between G. barbadense and G. hirsutum revealed that 8,245 unigenes from G. barbadense were detected the similarity with those released publicly in G. hirsutum, however, the remaining 408 sequences had no hits against G. hirsutum unigenes database. Furthermore, 13,275 putative ESTs InDels loci involved in the orthologous and/or homoeologous differences between/within G. barbadense and G. hirsutum were discovered by in silico analyses, and 2,160 InDel markers were developed by ESTs with more than five insertions or deletions. By gel electrophoresis combined with sequencing verification, 71.11% candidate InDel loci were reconfirmed orthologous and/or homoeologous loci polymorphisms using G. hirsutum acc TM-1 and G. barbadense cv Hai7124. Blastx result showed among 2,160 InDel loci, 81 with significant function similarity with known genes associated with secondary wall synthesis process, indicating the important roles in fiber quality in tetraploid cultivated cotton species.

Conclusion

Sequence comparisons and InDel markers development will lay the groundwork for promoting the identification of genes related to superior agronomic traits, genetic differentiation and comparative genomic studies between G. hirsutum and G. barbadense.
Transcriptomics of morphological color change in polychromatic Midas cichlids
Authors: Henning Frederico , Jones Julia C , Franchini Paolo , Meyer Axel
Published: March 2013

Background

Animal pigmentation has received much attention in evolutionary biology research due to its strong implications for adaptation and speciation. However, apart from a few cases the genetic changes associated with these evolutionary processes remain largely unknown. The Midas cichlid fish from Central America are an ideal model system for investigating pigmentation traits that may also play a role in speciation. Most Midas cichlids maintain their melanophores and exhibit a grayish (normal) color pattern throughout their lives. A minority of individuals, however, undergo color change and exhibit a distinctive gold or even white coloration in adulthood. The ontogenetic color change in the Midas cichlids may also shed light on the molecular mechanisms underlying pigmentation disorders in humans.

Results

Here we use next-generation sequencing (Illumina) RNAseq analyses to compare skin transcriptome-wide expression levels in three distinct stages of color transformation in Midas cichlids. cDNA libraries of scale tissue, for six biological replicates of each group, were generated and sequenced using Illumina technology. Using a combination of three differential expression (DE) analyses we identified 46 candidate genes that showed DE between the color morphs. We find evidence for two key DE patterns: a) genes involved in melanosomal pathways are up-regulated in normally pigmented fish; and b) immediate early and inflammatory response genes were up-regulated in transitional fish, a response that parallels some human skin disorders such as melanoma formation and psoriasis. One of the DE genes segregates with the gold phenotype in a genetic cross and might be associated with incipient speciation in this highly “species-rich” lineage of cichlids.

Conclusions

Using transcriptomic analyses we successfully identified key expression differences between different color morphs of Midas cichlid fish. These differentially expressed genes have important implications for our understanding of the molecular mechanisms underlying speciation in this lineage of extremely young species since they mate strongly assortatively, and new species may arise by sexual selection due to this color polymorphism. Some of the human orthologues of the genes identified here may also be involved in pigmentation differences and diseases and therefore provide genetic markers for the detection of human pigmentation disorders.
Recognition of face-name associations after errorless and errorful learning: an fMRI study
Authors: Hammer Anke , Tempelmann Claus , Münte Thomas F
Published: March 2013

Background

Errorless learning has advantages over errorful learning. The erroneous items produced during errorful learning compete with correct items at retrieval resulting in decreased memory performance. This interference is associated with an increased demand on executive monitoring processes. Event-related functional magnetic resonance imaging (fMRI) was used to contrast errorless and errorful learning. Learning mode was manipulated by the number of distractors during learning of face-name associations: in errorless learning only the correct name was introduced. During errorful learning either one incorrect name or two incorrect names were additionally introduced in order to modulate the interference in recognition.

Results

The behavioural results showed an enhanced memory performance after errorless learning. The veridicality of recognition of the face-name associations was reflected in a left lateralized fronto-temporal-parietal network. The different learning modes were associated with modulations in left prefrontal and parietal regions.

Conclusions

Errorless learning enhances memory performance as compared to errorful learning and underpins the known advantages for errorless learning. During memory retrieval different networks are engaged for specific purposes: Recognition of face-name associations engaged a lateralized fronto-temporal-parietal network and executive monitoring processes of memory engaged the left prefrontal and parietal regions.
Beclin1 inhibition promotes autophagy and decreases gemcitabine–induced apoptosis in Miapaca2 pancreatic cancer cells
Authors: Li Xiaoshu , Yan Jun , Wang Lisheng , Xiao Fengjun , Yang Yuefeng , Guo Xiaozhong , Wang Hua
Published: March 2013

Background

Beclin1 is a well-known key regulator of autophagy, which is also a haploinsufficient tumor suppressor. Current studies revealed that down-regulation or monoallelic deletions of Beclin1 were frequently found in various cancers. The purpose of this study was to investigate the effects of Beclin1 inhibition on autophagy and Gemcitabine-induced apoptosis of pancreatic cancer cells.

Methods

Beclin1 expression was inhibited by siRNA transduction and gene expression was determined by Real-time PCR and Western blot. The effects of Beclin1 inhibition on autophagy and Gemcitabine-induced apoptosis of Miapaca2 cells were analyed through LC3 expression, cell viability, cell cycle and apoptosis by using Western blot.

Results

We observed that Beclin1 silence promoted microtubule-associated protein 1 light chain 3-II (LC3-II) protein formation and increased punctate fluorescent signals in Miapaca2 cells transfected with green fluorescent protein (GFP)-tagged LC3. Beclin1 inhibition showed a greater suppressive effect on Gemcitabine-induced apoptosis of Miapaca2 cells.

Conclusion

Our data suggested that Beclin1 silence not only up-adjusted autophagy process, but also played an important role in the regulation of apoptosis. Beclin1 inhibition could inhibit apoptosis signaling induced by Gemcitabine in Miapaca2 cells.
Synchronous triple carcinoma of the colon and rectum
Authors: Yeh Chien-Chih , Hsi Sheng-Chuan , Chuu Chih-Pin , Kao Yung-Hsi
Published: March 2013

Synchronous multiple colorectal cancers are defined as multiple malignant colorectal tumors that occur simultaneously. All tumors are distant from each other, and none are the result of metastasis from other tumors. Here, we present a case of a 79-year-old man who was admitted to our hospital because of a 3-month history of abdominal pain associated with anemia, loss of appetite, and body weight loss. The patient did not have a family history of cancer. Computed tomography revealed bowel wall thickness and mesentery inflammation at the hepatic flexure of the colon and cecum. Colonoscopy revealed a tumor located 10 cm from the anal verge. Colonoscopic examination of the large bowel was not possible because of bowel obstruction due to the rectal tumor. Synchronous triple adenocarcinoma of the colon and rectum was confirmed by pathologic examination. The tumor was surgically resected by two-segment resection of the colon, low anterior resection, and right hemicolectomy. We used intraoperative colonoscopy to confirm that there were no other lesions after the resection of the three tumors. To the best of our knowledge, this is the first case of synchronous triple carcinoma of the colon and rectum in Taiwan. We consider that comprehensive preoperative study, extensive intraoperative exploration, and radical resection can increase the survival rate of patients with synchronous multiple colorectal cancers.
Neurodevelopmental alcohol exposure elicits long-term changes to gene expression that alter distinct molecular pathways dependent on timing of exposure
Authors: Kleiber Morgan L , Mantha Katarzyna , Stringer Randa L , Singh Shiva M
Published: March 2013

Background

Maternal alcohol consumption is known to adversely affect fetal neurodevelopment. While it is known that alcohol dose and timing play a role in the cognitive and behavioral changes associated with prenatal alcohol exposure, it is unclear what developmental processes are disrupted that may lead to these phenotypes.

Methods

Mice (n=6 per treatment per developmental time) were exposed to two acute doses of alcohol (5 g/kg) at neurodevelopmental times representing the human first, second, or third trimester equivalent. Mice were reared to adulthood and changes to their adult brain transcriptome were assessed using expression arrays. These were then categorized based on Gene Ontology annotations, canonical pathway associations, and relationships to interacting molecules.

Results

The results suggest that ethanol disrupts biological processes that are actively occurring at the time of exposure. These include cell proliferation during trimester one, cell migration and differentiation during trimester two, and cellular communication and neurotransmission during trimester three. Further, although ethanol altered a distinct set of genes depending on developmental timing, many of these show interrelatedness and can be associated with one another via ‘hub’ molecules and pathways such as those related tohuntingtinandbrain-derived neurotrophic factor.

Conclusions

These changes to brain gene expression represent a ‘molecular footprint’ of neurodevelopmental alcohol exposure that is long-lasting and correlates with active processes disrupted at the time of exposure. This study provides further support that there is no neurodevelopmental time when alcohol cannot adversely affect the developing brain.
Segregation mechanisms of tissue cells: from experimental data to models
Authors: Méhes Előd , Vicsek Tamás
Published: March 2013

Considerable advance has been made in recent years in the research field of pattern formation by segregation of tissue cells. Research has become more quantitative partly due to more in-depth analysis of experimental data and the emergence modeling approaches. In this review we present experimental observations, including some of our new results, on various aspects of two and three dimensional segregation events and then summarize the computational modeling approaches.
Flow-dependent porosity and other biomechanical properties of mysticete baleen
Authors: Werth Alexander J.
Published: March 2013

Despite its vital function in a highly dynamic environment, baleen is typically assumed to be a static material. Its biomechanical and material properties have not previously been explored. Thus I tested sections of baleen from bowhead whales, Balaena mysticetus, and humpback whales, Megaptera novaeangliae, alone or in groups representing miniature ‘racks’, in a flow tank through which water and buoyant particles circulated with variable flow velocity. Kinematic sequences were recorded through an endoscopic camera or viewing window. One set of experiments investigated particle capture; another series analyzed biomechanical behavior, including fringe spacing, movement and interaction. Baleen fringe porosity directly correlates, in a mostly linear fashion, with velocity of incident water flow. However, undulation and interaction of fringes (especially of bowheads) at higher flow velocities can decrease porosity. Fringe porosity depends on distance from the baleen plate. Porosity also varies, with fringe length, by position along the length of an individual plate. Plate orientation, which varied from 0 to 90 deg relative to water flow, is crucial in fringe spacing and particle capture. At all flow velocities, porosity is lowest with plates aligned parallel to water flow. Turbulence introduced when plates rotate perpendicular to flow (as in cross-flow filtration) increases fringe interaction, so that particles more easily strike fringes yet more readily dislodge. Baleen of bowhead whales, which feed by continuous ram filtration, differs biomechanically from that of humpbacks, which use intermittent lunge filtration. The longer, finer fringes of bowhead baleen readily form a mesh-like mat, especially at higher flow velocities, to trap tiny particles.
Risk Factors for Venous Thromboembolism in Patients with Chronic Liver Disease
Authors: Walsh Kelly A , Lewis Daniel A , Clifford Timothy M , Hundley Jonathan C , Gokun Yevgeniya , Angulo Paul , Davis George A
Published: March 2013

BACKGROUND

Pharmacologic prophylaxis for venous thromboembolism (VTE) in patients with chronic liver disease (CLD) presents a unique challenge because of coagulopathies associated with the disease. When evaluating whether these patients require VTE prophylaxis upon hospitalization, it would be advantageous if risk factors for the development of VTE in this population were known.

OBJECTIVE

To evaluate risk factors associated with the development of VTE in patients with CLD.

METHODS

A retrospective case-control study was conducted. Patients admitted to the University of Kentucky Chandler Hospital from October 2006 to July 2010 with a diagnosis of CLD and VTE were matched in a 1:3 fashion with CLD patients without VTE. The primary objective was to determine whether there were significant differences in laboratory values between the 2 groups.

RESULTS

During this time, 27 patients with CLD (1.0%) were diagnosed with VTE. These patients had significantly lower median aspartate aminotransferase (AST) (47 vs 70 U/L, p = 0.04), alanine transaminase (ALT) (24.5 vs 36 U/L, p = 0.02), albumin (2.1 vs 2.4 g/dL, p = 0.02) and hematocrit (Hct) (28.3% vs 32%, p = 0.03) values compared to the control patients. Patients with albumin lower than 1.9 g/dL had a 5.1 times greater risk of VTE compared to patients with albumin of 2.8 g/dL and higher (OR 5.14, 95% CI 1.05–25.2).

CONCLUSIONS

Patients with CLD who developed VTE had significantly lower AST, ALT, albumin, and Hct compared to those of control patients. Studies are necessary to further examine the significance of this finding.
Intermittent Nondaily Dosing Strategies in Patients with Previous Statin-Induced Myopathy
Authors: Keating Alyssa J , Campbell Kristen Bova , Guyton John R
Published: March 2013

OBJECTIVE

To review the safety and efficacy of alternative intermittent statin dosing regimens in patients with previous intolerance due to myopathy.

DATA SOURCES

Literature was accessed through MEDLINE (1946 -December 2012) and EMBASE (1966-December 2012) using relevant MeSH and Emtree search terms, including statins, HMG Co-A reductase inhibitors, simvastatin, atorvastatin, lovastatin, fluvastatin, pravastatin, pitavastatin, rosuvastatin, myopathy, and myalgias. Web of Science (1955-December 2012) and the aforementioned databases were additionally searched using combinations of the following text words: statin intolerance, alternate dosing, nondaily dosing, weekly dosing, statin-induced myopathy, and intermittent statin dosing. References of identified articles were reviewed for additional citations.

STUDY SELECTION AND DATA EXTRACTION

All identified English-language peer-reviewed publications were evaluated. Articles (excluding meeting abstracts) specifically addressing nondaily statin use in patients with previous statin-induced myopathy were reviewed.

DATA SYNTHESIS

Although statins have achieved significant reductions in cardiovascular morbidity and mortality, as many as 10% of patients prescribed these therapies experience myopathies. Intermittent statin regimens ranging from every-other-day to once- weekly dosing have emerged in an attempt to maintain efficacy while moderating the incidence of adverse effects. The results reported in 10 publications investigating varying regimens with atorvastatin and/or rosuvastatin revealed that at least 70% of patients were able to tolerate an intermittent dosing strategy without a recurrence of previous treatment-limiting adverse effects. Although the degree of low-density lipoprotein cholesterol–lowering varied appreciably among studies (12–38%), the addition of a nondaily statin regimen facilitated attainment of National Cholesterol Education Program goals for some.

CONCLUSIONS

Although areas of uncertainty remain, intermittent dosing (particularly with rosuvastatin and atorvastatin) in previously intolerant patients is a useful strategy to capitalize on the benefits of this therapy. Larger scale randomized trials are necessary to more clearly define the role of this strategy and the optimal choice of regimen.
Aziridinyl imines in organic synthesis: Development of tandem reaction strategies and application to total synthesis of natural products
Authors: Lee Hee-Yoon , Song Seog-Beom , Kang Taek , Kim Yoon Jung , Geum Su Jeong
Published: March 2013

Aziridinyl imines are well-known carbene equivalents because they are precursors of diazo compounds from which reactive intermediates can be produced. These carbene equivalents can be utilized as zwitterionic species, diradicals, or 4π system for cycloaddition reactions. Thus, the intermediates derived from aziridinyl imines have been used in the sulfur-ylide-mediated epoxide formation, tandem free-radical reactions, or cyclopropanation reaction via carbene intermediates to form trimethylenemethane (TMM) diyls, which undergo [2 + 3] cycloaddition reactions to form cyclopentanoids. Diazo compounds generated from aziridinyl imines also react with allenes to form TMM diyls. This reaction was utilized in tandem cycloaddition reactions of linear substrates to form polyquinanes. These tandem reaction strategies were successfully applied to the total synthesis of various cyclopentanoid natural products.
Transient Receptor Potential Vanilloid Type 1 Channel (TRPV1) Immunolocalization in the Murine Enteric Nervous System Is Affected by the Targeted C-terminal Epitope of the Applied Antibody
Authors: Buckinx Roeland , Van Nassauw Luc , Avula Leela R. , Alpaerts Katrien , Adriaensen Dirk , Timmermans Jean-Pierre
Published: March 2013

The expression of transient receptor potential vanilloid type 1 channel (TRPV1) in the enteric nervous system is still the subject of debate. Although a number of studies have reported that TRPV1 is limited to extrinsic afferent fibers, other studies argue for an intrinsic expression of TRPV1. In the present study, reverse transcriptase PCR was employed to establish the expression of TRPV1 mRNA throughout the gastrointestinal tract. Using two antibodies directed against different epitopes of TRPV1, we were able to show at the protein level that the observed distribution pattern of TRPV1 is dependent on the antibody used in the immunohistochemical staining. A first antibody indeed mainly stained neuronal fibers, whereas a second antibody exclusively stained perikarya of enteric neurons throughout the mouse gastrointestinal tract. We argue that these different distribution patterns are due to the antibodies discriminating between different modulated forms of TRPV1 that influence the recognition of the targeted immunogen and as such distinguish intracellular from plasmalemmal forms of TRPV1. Our study is the first to directly compare these two antibodies within the same species and in identical conditions. Our observations underline that detailed knowledge of the epitope that is recognized by the antibodies employed in immunohistochemical procedures is a prerequisite for correctly interpreting experimental results.
Comparing the Relative Effectiveness of Advertising Channels: A Case Study of a Multimedia Blitz Campaign
Authors: Danaher Peter J. , Dagger Tracey S.
Published: March 2013

In this study, the authors develop an inexpensive method to help firms assess the relative effectiveness of multiple advertising media. Specifically, they use a firm's loyalty program database to capture media exposure, through an online media survey, for all the media in which the firm advertises. In turn, the exposure data are matched with the purchase history for these same respondents, thereby creating single-source data. The authors illustrate their method for a large retailer that undertook a short-term promotional sale by advertising in television, radio, newspaper, magazine, online display ad, sponsored search, social media, catalog, direct mail, and e-mail channels. In this case, seven of the ten media significantly influence purchase outcomes. Finally, the authors demonstrate how to use their advertising response model to determine the optimal budget allocation across each advertising media channel.
Adolescents Are More Vulnerable to Cocaine Addiction: Behavioral and Electrophysiological Evidence
Authors: Wong Wai Chong , Ford Kerstin A. , Pagels Nicole E. , McCutcheon James E. , Marinelli Michela
Published: March 2013

In humans, adolescence is a period of heightened propensity to develop cocaine addiction. It is unknown whether this is attributable to greater access and exposure to cocaine at this age, or whether the adolescent brain is particularly vulnerable to the addictive properties of cocaine. Here, we subjected male adolescent (P42) and adult (∼P88) rats to a wide range of cocaine self-administration procedures. In addition, to determine whether behavioral differences are associated with developmental differences in dopaminergic activity, we examined and manipulated the activity of dopamine neurons. Relative to adults, adolescent rats took cocaine more readily, were more sensitive to lower doses, showed greater escalation of cocaine intake, and were less susceptible to increases in price (i.e., were more “inelastic”). In parallel, adolescents also showed elevated activity of ventral tegmental area dopamine neurons, a feature known to be associated with increased self-administration behavior. Pharmacological manipulation of dopamine D2receptor function with quinpirole (agonist) or eticlopride (antagonist), to alter dopamine neuron activity, eliminated age differences in cocaine self-administration. These data suggest a causal relationship between behavioral and electrophysiological determinants of cocaine addiction liability. In conclusion, adolescents show behavioral and electrophysiological traits of heightened addiction liability.
Intracellular Magnesium-Dependent Modulation of Gap Junction Channels Formed by Neuronal Connexin36
Authors: Palacios-Prado Nicolás , Hoge Gregory , Marandykina Alina , Rimkute Lina , Chapuis Sandrine , Paulauskas Nerijus , Skeberdis Vytenis A. , O'Brien John , Pereda Alberto E. , Bennett Michael V. L. , Bukauskas Feliksas F.
Published: March 2013

Gap junction (GJ) channels composed of Connexin36 (Cx36) are widely expressed in the mammalian CNS and form electrical synapses between neurons. Here we describe a novel modulatory mechanism of Cx36 GJ channels dependent on intracellular free magnesium ([Mg2+]i). We examined junctional conductance (gj) and its dependence on transjunctional voltage (Vj) at different [Mg2+]iin cultures of HeLa or N2A cells expressing Cx36. We found that Cx36 GJs are partially inhibited at resting [Mg2+]i. Thus,gjcan be augmented or reduced by lowering or increasing [Mg2+]i, respectively. Similar changes ingjandVj-gating were observed using MgATP or K2ATP in pipette solutions, which increases or decreases [Mg2+]i, respectively. Changes in phosphorylation of Cx36 or in intracellular free calcium concentration were not involved in the observed Mg2+-dependent modulation ofgj. Magnesium ions permeate the channel and transjunctional asymmetry in [Mg2+]iresulted in asymmetricVj-gating. Thegjof GJs formed of Cx26, Cx32, Cx43, Cx45, and Cx47 was also reduced by increasing [Mg2+]i, but was not increased by lowering [Mg2+]i; single-channel conductance did not change. We showed that [Mg2+]iaffects both open probability and the number of functional channels, likely through binding in the channel lumen. Finally, we showed that Cx36-containing electrical synapses between neurons of the trigeminal mesencephalic nucleus in rat brain slices are similarly affected by changes in [Mg2+]i. Thus, this novel modulatory mechanism could underlie changes in neuronal synchronization under conditions in which ATP levels, and consequently [Mg2+]i, are modified.
Limited Recovery of Pineal Function after Regeneration of Preganglionic Sympathetic Axons: Evidence for Loss of Ganglionic Synaptic Specificity
Authors: Lingappa Jaisri R. , Zigmond Richard E.
Published: March 2013

The cervical sympathetic trunks (CSTs) contain axons of preganglionic neurons that innervate the superior cervical ganglia (SCGs). Because regeneration of CST fibers can be extensive and can reestablish certain specific patterns of SCG connections, restoration of end organ function would be expected. This expectation was examined with respect to the pineal gland, an organ innervated by the two SCGs. The activity of pineal serotoninN-acetyltransferase (NAT) exhibits a large circadian rhythm that is dependent on the sympathetic input of the gland, with high activity at night. Thirty-six hours after the CSTs were crushed bilaterally, nocturnal NAT was decreased by 99%. Three months later, enzyme activity had recovered only to 15% of control values, a recovery dependent on regeneration of CST fibers. Nevertheless, a small day/night rhythm was present in lesioned animals. Neither the density of the adrenergic innervation of the gland nor the ability of an adrenergic agonist to stimulate NAT activity was reduced in rats with regenerated CSTs. In addition, stimulation of the regenerated CST at a variety of frequencies was at least as effective in increasing NAT activity as seen with control nerves. These data suggest that the failure of pineal function to recover is not attributable to a quantitative deficit in the extent of reinnervation or synaptic efficacy. Rather, we suggest that there is some loss of specificity in the synaptic connections made in the SCG during reinnervation, resulting in a loss of the central neuronal information necessary for directing a normal NAT rhythm and thus normal pineal function.
Causal Frequency-Specific Contributions of Frontal Spatiotemporal Patterns Induced by Non-Invasive Neurostimulation to Human Visual Performance
Authors: Chanes Lorena , Quentin Romain , Tallon-Baudry Catherine , Valero-Cabré Antoni
Published: March 2013

Neural oscillatory activity is known to play a crucial role in brain function. In the particular domain of visual perception, specific frequency bands in different brain regions and networks, from sensory areas to large-scale frontoparietal systems, have been associated with distinct aspects of visual behavior. Nonetheless, their contributions to human visual cognition remain to be causally demonstrated. We hereby used non-uniform (and thus non-frequency-specific) and uniform (frequency-specific) high-beta and gamma patterns of noninvasive neurostimulation over the right frontal eye field (FEF) to isolate the behavioral effects of oscillation frequency and provide causal evidence that distinct visual behavioral outcomes could be modulated by frequency-specific activity emerging from a single cortical region. In a visual detection task using near-threshold targets, high-beta frequency enhanced perceptual sensitivity (d′) without changing response criterion (beta), whereas gamma frequency shifted response criterion but showed no effects on perceptual sensitivity. The lack of behavioral modulations by non-frequency-specific patterns demonstrates that these behavioral effects were specifically driven by burst frequency. We hypothesize that such frequency-coded behavioral impact of oscillatory activity may reflect a general brain mechanism to multiplex functions within the same neural substrate. Furthermore, pathological conditions involving impaired cerebral oscillations could potentially benefit in the near future from the use of neurostimulation to restore the characteristic oscillatory patterns of healthy systems.
Diverse Visual Features Encoded in Mouse Lateral Geniculate Nucleus
Authors: Piscopo Denise M. , El-Danaf Rana N. , Huberman Andrew D. , Niell Cristopher M.
Published: March 2013

The thalamus is crucial in determining the sensory information conveyed to cortex. In the visual system, the thalamic lateral geniculate nucleus (LGN) is generally thought to encode simple center-surround receptive fields, which are combined into more sophisticated features in cortex, such as orientation and direction selectivity. However, recent evidence suggests that a more diverse set of retinal ganglion cells projects to the LGN. We therefore used multisite extracellular recordings to define the repertoire of visual features represented in the LGN of mouse, an emerging model for visual processing. In addition to center-surround cells, we discovered a substantial population with more selective coding properties, including direction and orientation selectivity, as well as neurons that signal absence of contrast in a visual scene. The direction and orientation selective neurons were enriched in regions that match the termination zones of direction selective ganglion cells from the retina, suggesting a source for their tuning. Together, these data demonstrate that the mouse LGN contains a far more elaborate representation of the visual scene than current models posit. These findings should therefore have a significant impact on our understanding of the computations performed in mouse visual cortex.
Intrauterine growth restriction alters term fetal baboon hypothalamic appetitive peptide balance
Authors: Li Cun , McDonald Thomas J , Wu Guoyao , Nijland Mark J , Nathanielsz Peter W
Published: March 2013

Neurons controlling appetite are located in the hypothalamic arcuate nuclei (ARH). Offspring appetite regulation has been shown to be modified by dysregulation of ARH nuclear development. Most ARH developmental studies have been in altricial rodents whose hypothalamic development is predominantly postnatal. In primates including humans, much development of hypothalamic appetite regulatory centers occurs before birth. We hypothesized that i) appetitive peptides are abundantly expressed by 90 percent gestation (0.9G), ready for postnatal function; ii) by 0.9G, intrauterine growth restriction (IUGR) increases the orexigenic:anorexigenic peptide ratio; iii) IUGR increases fetal glucocorticoid receptor (GR) expression; and iv) IUGR decreases STAT3, which signals inhibition of appetite. We developed a fetal baboon IUGR model resulting from reduced maternal nutrition. Pregnant baboons were fed ad libitum, controls (CTR; n=24), or 70% CTR diet to produce IUGR (n=14). C-section was performed at 0.9G. In CTR (n=7) and IUGR (n=6) fetal brains, ARH appetite regulatory peptides (neuropeptide Y (NPY) and proopiomelanocortin (POMC)) were quantified immunohistochemically. Fetal plasma cortisol was raised in IUGR fetuses. We observed that NPY and POMC were well expressed by 0.9G. IUGR increased NPY, GR, and active phosphorylated GR and decreased POMC and phosphorylated form of STAT3. We conclude that IUGR dysregulates ARH development in ways that will reset the appetitive neuropeptide balance in favor of increased appetite drive in postnatal life. We postulate that changes in peptide abundance are in part due to increased fetal cortisol and ARH GR. These changes may contribute to predisposition to obesity in IUGR offspring.
Charging behavior of MNOS and SONOS memory structures with embedded semiconductor nanocrystals - Computer simulation
Authors: Molnár K. Z. , Turmezei P. , Horváth Zs. J.
Published: March 2013

Papers in the Appendix were published in electronic format as Volume 1534
Features of Electronic Transport in relaxed Si/Si1-XGeX Heterostructures with High doping level
Authors: Orlov Lev K. , Mel’nikova A. A. , Orlov Mikhail L. , Alyabina Natal’ya A. , Ivina Natal’ya L. , Neverov V. N. , Horváth Zsolt J.
Published: March 2013

Papers in the Appendix were published in electronic format as Volume 1534
Effect of the Surface on Optical properties of AlGaAs/GaAs heterostructures with double 2-DEG
Authors: Zamora-Peredo L. , Cortes-Mestizo I. , García-Gonzalez L. , Hernandez-Torres J. , Vázquez-Cortes D. , Shimomura S. , Cisneros A. , Méndez-García V. , Lopez-Lopez M.
Published: March 2013

Papers in the Appendix were published in electronic format as Volume 1534
Double core Infrared (CdSeTe) / ZnS quantum dots conjugated to Papiloma virus antibodies
Authors: Espinola J.L. Casas , Torchynska T. V. , Gómez J. A. Jaramillo , Douda J. , Gazarian K.
Published: March 2013

Papers in the Appendix were published in electronic format as Volume 1534
Thin Film Hybrid Ceramic-Polymeric Low Cost Solar Absorber
Authors: Chávez-Urbiola Edgar A. , Pérez-Robles Juan F.
Published: March 2013

Papers in the Appendix were published in electronic format as Volume 1534
Thermoelectric Measurements of Ni Nanojunctions
Authors: Lee See Kei , Yamada Ryo , Tada Hirokazu
Published: March 2013

We investigated the thermoelectric voltage (TEV) of atomic contacts of nickel (Ni) by using a scanning tunneling microscope. The TEV of nanoscale junctions show fluctuation in stepwise manner. Histogram analysis of TEV observed in the Ni point contact with the conductance of 1.2 G0 (G0 = 2e2/h is the quantum of charge conductance) revealed multiple voltage peaks at larger and smaller values observed at conductance of 2.5 G0, which showed a single sharp voltage peak. Fluctuation observed in our results suggest that there is transition of the transport channel distribution caused by the thermal motion of Ni atoms.
Influence of Metsulfuron-Containing Herbicides and Application Timings on Tall Fescue Seedhead Production and Forage Yield
Authors: Sather Bryan C. , Roberts Craig A. , Bradley Kevin W.
Published: March 2013

Field trials were conducted in 2009 and 2010 to investigate the effects of metsulfuron-containing herbicides on tall fescue growth, seedhead production, yield, and forage nutritive value. Several rates of metsulfuron-containing products and picloram plus 2,4-D were applied to a weed-free tall fescue hay field in the early spring vegetative, late spring boot, and late summer dormancy stages of growth. Compared to the nontreated control, applying metsulfuron-containing herbicides to vegetative tall fescue reduced plant height by 13 to 40% whereas boot-stage applications of these same herbicides reduced height by 28 to 45%. Metsulfuron-containing herbicides reduced seedhead density from 14 to 61% when applied to vegetative tall fescue, and from 53 to 88% when applied at the boot stage. Metsulfuron plus 2,4-D plus dicamba (0.01 + 0.40 + 0.14 kg ai ha−1) was the only metsulfuron-containing treatment applied at the vegetative application timing that did not reduce tall fescue seedheads or yield when compared to the nontreated control. Vegetative-stage applications of metsulfuron-containing herbicides reduced tall fescue yields by 33 to 63%, whereas boot-stage applications reduced yields by 15 to 35%. Picloram plus 2,4-D did not reduce tall fescue height, seedhead density, or yield when applied at either timing. Tall fescue crude protein (CP) concentration was greater in response to the vegetative compared to boot-stage herbicide applications, and vegetative-stage applications of metsulfuron-containing herbicides increased CP concentration of tall fescue by 1.5 to 3.4% compared to the nontreated control. Results from these experiments indicate that spring applications of metsulfuron-containing herbicides can be utilized to reduce tall fescue seedhead production and increase CP content of tall fescue pastures and hay fields, but summer applications of these same herbicide treatments will have only limited effects on yield, nutritive values, or seedhead density of tall fescue harvested in the fall or the spring following treatment.
Cover Crops for Weed Management in Southern Reduced-Tillage Vegetable Cropping Systems
Authors: Price Andrew J. , Norsworthy Jason. K.
Published: March 2013

With growing agricultural demands from both conventional and organic systems comes the need for sustainable practices to ensure long-term productivity. Implementation of reduced- or no-till practices offers a number of environmental benefits for agricultural land and maintains adequate yield for current and future production. Concerns over satisfactory pest control options, particularly weed control, have contributed to the slow adoption of conservation practices in many areas. To identify effective alternative weed management options for use in conservation systems, research in the Southeast has continued to evaluate the use of cover crops in conjunction with reduced-tillage practices. A number of cover crop species, including cereal grains, legumes, and Brassicaceae species, that have potential to suppress weeds through direct crop interference or allelopathic potential have been investigated. Many recent research projects in the Midsouth and southeastern United States have assessed the success of cover crops in reduced-tillage row crop settings with promising outcomes in some systems. However, continued research is necessary to identify appropriate cover crop and tillage systems for use in other agricultural settings, such as vegetable crops and organic production systems.
European Perspectives on the Adoption of Nonchemical Weed Management in Reduced-Tillage Systems for Arable Crops
Authors: Melander Bo , Munier-Jolain Nicolas , Charles Raphaël , Wirth Judith , Schwarz Jürgen , van der Weide Rommie , Bonin Ludovic , Jensen Peter K. , Kudsk Per
Published: March 2013

Noninversion tillage with tine- or disc-based cultivations prior to crop establishment is the most common way of reducing tillage for arable cropping systems with small grain cereals, oilseed rape, and maize in Europe. However, new regulations on pesticide use might hinder further expansion of reduced-tillage systems. European agriculture is asked to become less dependent on pesticides and promote crop protection programs based on integrated pest management (IPM) principles. Conventional noninversion tillage systems rely entirely on the availability of glyphosate products, and herbicide consumption is mostly higher compared to plow-based cropping systems. Annual grass weeds and catchweed bedstraw often constitute the principal weed problems in noninversion tillage systems, and crop rotations concurrently have very high proportions of winter cereals. There is a need to redesign cropping systems to allow for more diversification of the crop rotations to combat these weed problems with less herbicide input. Cover crops, stubble management strategies, and tactics that strengthen crop growth relative to weed growth are also seen as important components in future IPM systems, but their impact in noninversion tillage systems needs validation. Direct mechanical weed control methods based on rotating weeding devices such as rotary hoes could become useful in reduced-tillage systems where more crop residues and less workable soils are more prevalent, but further development is needed for effective application. Owing to the frequent use of glyphosate in reduced-tillage systems, perennial weeds are not particularly problematic. However, results from organic cropping systems clearly reveal that desisting from glyphosate use inevitably leads to more problems with perennials, which need to be addressed in future research.
Temperature and Relative Humidity Affect Weed Response to Vinegar and Clove Oil
Authors: Brainard Daniel C. , Curran William S. , Bellinder Robin R. , Ngouajio Mathieu , VanGessel Mark J. , Haar Milton J. , Lanini W. Thomas , Masiunas John B.
Published: March 2013

Nonsynthetic herbicides offer a potentially useful addition to the suite of weed management tools available to organic growers, but limited information is available to guide the optimal use of these products. The objectives of this research were to (1) evaluate the efficacy of clove oil– and vinegar-based herbicides on weeds across multiple states, and (2) assess the potential role of temperature, relative humidity (RH), and cloud cover in explaining inter-state variations in results. From 2006 to 2008, a total of 20 field trials were conducted in seven states using an identical protocol. Seeds of brown mustard were sown and herbicides applied to both mustard and emerged weeds when mustard reached the three- to four-leaf stage. Treatments included clove oil at 2.5, 5, 7.5, and 10% v/v concentrations at 54 L ha−1, and vinegar at 5, 10, 15, and 20% v/v concentrations at 107 L ha−1. Results varied widely across trials. In general, concentrations of at least 7.5% for clove oil and 15% for vinegar were needed for adequate control of mustard. Both products were more effective at suppressing mustard thanAmaranthusspp. or common lambsquarters. Poor control was observed for annual grasses. No significant effects of cloud cover on the efficacy of either product were detected. In contrast, RH was positively correlated with control of brown mustard by both clove oil and vinegar with improved control at higher RH. Temperature had no detectable effect on the efficacy of clove oil, but higher temperatures improved control of brown mustard by vinegar.
Influence of Two Herbicides on Soybean Cyst Nematode (Heterodera glycines) Reproduction on Henbit (Lamium amplexicaule) Roots
Authors: Werle Rodrigo , Bernards Mark L. , Giesler Loren J. , Lindquist John L.
Published: March 2013

Soybean cyst nematode (SCN) is the most yield-limiting pathogen of soybean in the United States. Henbit is a prevalent winter annual weed species in no-till fields and is reported to be an alternative host of SCN. A greenhouse study was conducted to evaluate how the development of SCN on henbit roots was affected by herbicide mode of action and time of herbicide application. Henbit plants were grown in watertight pots placed in a water bath bench that kept soil temperature constant (27 ± 1 C) during the study. Ten d after transplanting, pots were inoculated with approximately 1,000 SCN eggs. At 7, 14, or 21 d after inoculation (DAI), henbit plants were sprayed with recommended dose of either glyphosate (870 g ae ha−1) or 2,4-D (1,070 g ae ha−1). The experiment was arranged in a randomized complete block design with five replications per treatment, and two experimental runs separated in time. At 28 DAI, the total number of SCN cysts and eggs, and plant shoot and root dry weight per pot were determined. Henbit root and shoot biomass increased as the time of herbicide application was delayed. Glyphosate reduced root biomass more than 2,4-D, but no differences in shoot biomass were detected. The number of SCN cysts per henbit plant and eggs per cyst increased as the herbicide application was delayed from 7 to 21 DAI. Glyphosate reduced the number of cysts found on henbit roots more than 2,4-D, especially at earlier application times. On plants treated with glyphosate, SCN-females produced only half the number of eggs of SCN-females on henbit plants treated with 2,4-D, regardless of time of application. These results indicate that early control of henbit plants, especially with glyphosate, can reduce SCN reproduction potential in SCN infested fields.