bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative analysis of dislocation rates for total hip replacement arthroplasty using fixed bearing acetabular cup and dual mobility acetabular cup in patients with hip dysplasia
Authors: Kim Jung Shin , Moon Nam Hoon , Do Min Uk , Jung Sung Won , Suh Kuen Tak , Shin Won Chul
Published: March 2023

Background
Total hip replacement arthroplasty (THA) in hip dysplasia patients has a higher complication rate than in patients with simple hip osteoarthritis due to anatomical deformation and previous surgical history. Hence the associated complications need to be probed.
Methods
From 2015 to 2020, 1525 patients underwent THA performed by two surgeons at a single institution. A total of 152 patients involving 172 THAs were included. Hip dysplasia was evaluated radiologically using Crowe classification. The patients were classified into dual-mobility (DM) and fixed-bearing (FB) acetabular cup groups. The occurrence of postoperative complications, including dislocation and functional evaluation of the hip joint, was analyzed before and after surgery using the modified Harris hip score.
Results
There was no difference in the preoperative demographics and radiographic parameters between the groups. The size of the external bearing of the DM acetabular cup (41.83 ± 2.66 mm) and the size of the femoral head implant in the FB acetabular cup (33.03 ± 1.99 mm) were significantly different (P value < 0.001). The incidence of postoperative hip dislocation was significantly lower in the DM group (DM 0% vs. FB 9.0%) (P value = 0.003). The hip joint function score showed no difference before surgery (DM 42.92 vs FB 50.12) or 2 years after surgery (DM 91.80 vs FB 92.03).
Conclusion
Treating hip dysplasia patients with THA using a dual-mobility acetabular cup can reduce postoperative dislocations, and could be used for the better management of these patients.
Effect of doxorubicin on viability and Bax, Bcl-2, p53, Nanog, Sox-2, Oct-4 gene expressions in MCF-7 stem-like cells
Authors: Andac Cenk A , Kocak Nadir , Dalan Altay Burak , Caglar Sena , Arslan Seyfullah O
Published: March 2023

Background: The aim of this study is to clarify whether cell viability, cell death, and gene expressions pertaining to self-renewal and pluripotency differ in doxorubicin (DOX) [IC50] treated and untreated human MCF-7 mammalian cancer cells as well as between their CD44+/CD24¯/low cancer stem cells (CSC).
Methods: Non-tumorigenic human MCF-10A mammalian cells and their CD44+/CD24¯/low stem cells were used as the control. Cell viability, apoptosis, necrosis and cell death were studied by flow cytometry. Cell death pathways, multidrug resistance, pluripotency and self-renewal were studied at Nanog, Oct-4, Sox-2, p53, Bcl-2 and Bax mRNA gene expression level by qRT-PCR.
Results: IC50 value for DOX treated MCF-7 cells was found to be 3.73 µM. Bax, Bcl-2, p53 genes were down-regulated while Nanog, Oct-4, Sox-2 genes were up-regulated in DOX [IC50] treated MCF-7 CSCs. Bax, p53, Nanog, Oct-4 genes were down-regulated while Sox-2, Bcl-2 genes were up-regulated in DOX [IC50] untreated MCF-7 CSCs.
Discussion: In addition to literature reports on DOX [IC50] treated non-stem MCF-7 cells undergoing autophagy and DOX [IC50] treated dedifferentiated MCF-7 (CD44+/CD24¯/low) cancer stem-like cells undergoing apoptosis, our laboratory data strongly suggest that DOX [IC50] treated MCF-7 CSCs also undergo necrosis as determined by flow cytometry and necroptosis due to downregulation of Bax, Bcl-2 and p53 genes.
Conclusion: Our finding suggests that multiple types of cell death pathways, including apoptosis, necrosis and necroptosis, is involved in DOX [IC50] treated MCF-7 CSCs. DOX [IC50] treated MCF-7 CSCs become pluripotent with self-renewal capability by up-regulation of Nanog, Oct-4, Sox-2 gene expressions to possibly survive necroptosis. Nanog, Oct-4, Sox-2 gene expressions are all down-regulated in DOX [IC50] treated MCF-10A (CD44+/CD24¯/low) stem cells, disabling the self-renewal and pluripotency features.
Full length transcriptome sequencing and differential expression gene analysis of Hybrid Larch under PEG simulated drought stress
Authors: Zhao Qingrong , Wang Chen , Zhang Sufang , Zhang Hanguo , Zhang Lei
Published: March 2023

Hybrid larch is one of the main afforestation and timber species in northeast China. Drought is one of the main factors limiting the growth of larch and other organisms. To further explore the mechanism of drought resistance of hybrid larch , the full-length sequencing of hybrid larch embryogenic callus under PEG simulated drought stress was carried out by combining Illumina-Hiseq and SMRT-seq. A total of 20.3Gb clean reads and 786492 CCS reads were obtained from the second and third generation sequencing. The de-redundant transcript sequences were predicted by lncRNA, 2083 lncRNAs were obtained, and the target genes were predicted, and a total of 2722 target genes were obtained. The de-redundant transcripts were further screened and 1654 differentially expressed genes (DEGs )were obtained. Among them, different DEGs respond to drought stress through different ways, such as Oxidation-reduction process, Starch and sucrose metabolism, Plant hormone pathway, Carbon metabolism, lignin catabolic/biosynthetic process and so on. This study provides basic full-length sequencing data for the study of Larch drought resistance, and excavates a large number of DEGs in response to drought stress, which helps us to further understand the function of Larch drought resistance genes and provides a reference for in-depth analysis of the molecular mechanism of Larch drought resistance.
Classification of the oesophageal perforation
Authors: Harrich Friederike H. M. , Knoefel Wolfram Trudo , Bölke Edwin , Schauer Matthias C.
Published: March 2023

Objectives
Oesophageal perforations are a complex clinical scenario that has been poorly studied.
To date, there is no staging of oesophageal perforation. The outcome is often fatal and heterogeneous, which is related to the fact that there is no classification and no treatment guideline.
Methods
The observation period of the study covered a period of four years. The cause of the injury and the time interval between the event and the start of therapy were analyzed. Therapy and the clinical signs of infection were evaluated.
Endpoints of the study were patient recovery or death due to sepsis.
The study was conducted as a retrospective single-center study in a maximum-care hospital and has been approved by the review board. Patients gave informed consent before data collection. All data were analyzed using SPSS (IBM SPSS Statistics software).
Results
For this study, oesophageal perforation cases from a maximum care hospital were summarized and classified for the first time, from which a prediction of lethality could be derived. The proposed endoscopic radiological classification correlated with survival in our study. Accordingly, we were able to suggest a therapeutic option to the different stages. It can be seen that lethality correlates significantly with the severity of the perforation. As a final result of the study a prediction of mortality at diagnosis is now possible.
Conclusion
Oesophageal perforations can be systematically classified into grades I to IV, with grade III divided into a, b, and c according to the height of the perforation in relation to the anatomy. A treatment guideline can now be discussed according to the severity of the perforation.
Cattle Manure Application for 12-17 Years Enhanced Depth Distribution of X-Ray Computed Tomography-Derived Soil Pore Characteristics
Authors: Sangotayo Anuoluwa Ojonoka , Chakraborty Poulamee , Xu Sutie , Kumar Sandeep , Kovacs Peter
Published: March 2023

Long-term manure and inorganic fertilizer application in row crops may significantly influence soil pore characteristics, thereby impacting soil aggregation and structure. Understanding the influence of such soil amendments on soil pore characteristics is useful to develop proper conservation practices. However, there is limited information on the impact of cattle manure and inorganic fertilizer application on soil pore characteristics at a microscale level in the soil profile. Therefore, in this study, the X-ray computed tomography (XCT) technique was utilized to quantify the impact of manure and fertilizer amendments under a corn (Zea mays L.)-soybean (Glycine max L.)-spring wheat (Triticum aestivum) rotation system on soil pore characteristics to 40 cm soil depth. The study was conducted at Brookings (initiated in 2008) and Beresford (2003) in South Dakota. The study treatments included: low manure (LM), medium manure (MM), high manure (HM), medium fertilizer (MF), high fertilizer (HF), and control (CK). Four replicated intact cores were collected from all the treatments at 0–10, 10–20, 20–30, and 30–40 cm depths. Image visualization and processing were performed using ImageJ software at a pixel resolution of (0.26 × 0.26 × 0.28) mm3. Data showed that treatments by depth interactions were mainly significant for soil organic carbon (SOC) content at 0–20 cm. The HM treatment increased the SOC by 8 to 68% compared to the CK and MF at 0–20 cm for either site. However, treatments did not always impact these parameters beyond 20 cm depth. Considering treatment as the main effect, the MM, HM, and HF increased the total number of pores (TP) compared to the CK at Beresford site. Soil depth impacted the TP and total number of macropores (Tmacro), where more Tmacro was observed at 0–10 cm compared to the 30–40 cm depth at Beresford site. In general, manure application improved SOC and TN contents and soil pore characteristics at 0–20 cm for both sites. This study illustrates the importance of the XCT technique in quantifying soil pore characteristics and highlighted the improvement in the XCT-derived pore characteristic with the long-term application of manure to a greater depth in the soil.
Public Perspectives on Strays and Companion Animal Management in Malaysia
Authors: Munir Syafiq Munir Ismail , Mokhtar Mohd Istajib , Arham Ahmad Firdhaus
Published: March 2023

Strays and companion animal management is a sensitive issue in Malaysia that incites solid and conflicting views. Through structured questionnaires administered to a random sample of 704 respondents, this study explored public opinion on a) causes of the stray animal population, b) the management of the stray animal population, and c) the national strategy on strays and companion Animal Management. The results show that 70.3% of respondents agreed that a lack of public awareness regarding animal care was the major contributor to the stray animal population. In addition, 58.1% of respondents felt that treating and vaccinating animals exposed to zoonotic diseases is a viable approach that could be instituted as a conceivable measure in stray animal population management. Finally, developing animal protection areas through a multi-stakeholder partnership strategy initiative recorded the highest support (48.4%) for intervention planning for stray animal management at a national level. Notably, a significant percentage of public responses were implicitly influenced by demographic variables. These findings provide valuable insights into public opinion regarding stray and companion animal management in Malaysia. These findings could inform the development of future legislation aimed at reducing the unfavorable effects of stray animal populations on humans and the ecology of Malaysia.
Altered dynamic functional and effective connectivity in drug-naive children with Tourette Syndrome
Authors: Qu Haibo , Luo Lekai , Liao Yi , Jia Fenglin , Ning Gang , Liu Jing , Li Xuesheng , Chen Xijian , Ma Xinmao , He Xuejia , Fu Chuan , Cai Xiaotang
Published: March 2023

Tourette syndrome (TS) is a developmental neuropsychiatric disorder characterized by repetitive, stereotyped, involuntary motor and vocal tics, the neurological basis of which remains unclear. Although traditional resting-state MRI (rfMRI) studies have identified abnormal static functional connectivity (FC) in patients with TS, dynamic FC (dFC) remains relatively unexplored. The rfMRI data of 54 children with TS and 46 typically developing children (TDC) were analyzed using group independent component analysis to obtain independent components (ICs), and a sliding-window approach to generate dFC matrices. All dFC matrices were clustered into two reoccurring states, the state transition metrics were obtained. We conducted Granger causality and degree centrality analyses to further investigate the brain regions that may play the most important roles in driving whole-brain switching between different states. We found that children with TS spent more time in state 2 (PFDR < 0.001), a state characterized by strong connectivity between ICs, and switched more quickly between states (PFDR = 0.025) than TDC. The default mode network (DMN) may play an important role in abnormal state transitions because the FC that changed the most between the two states was between the DMN and other networks. Additionally, the DMN had increased centrality and altered causal influence on other networks. Certain alterations related to cognitive function (r = – 0.309, P < 0.05) and tic symptom ratings (r = 0.282; 0.413, P < 0.05) may represent important aspects of the pathophysiology of TS. These findings facilitate our understanding of the neural basis for the clinical presentation of TS.
Dissociation of perception and motor execution of lower limb in multi-directional movements
Authors: Oku Kyosuke , Tanaka Shinsuke , Kida Noriyuki
Published: March 2023

Estimating action capability is important for humans to successfully move their bodies. Reachability has been proposed to be an overestimation of motor abilities, by judging the unreachable distance as reachable. Existing literature have mainly investigated the sagittal direction, but multi-directional reachability is unexplored. This study examined the relationship between cognition and movement using the reaching of the lower limbs in multiple directions. Sixteen adults were asked to reach targets projected on the floor in 21 locations (seven directions and three distances) to estimate the reaching time. We found that movement slowed as the direction increased toward the contralateral side, but subjective reaching time did not change with direction. Multiple regression analysis showed that subjective reaching time could be calculated with high accuracy, using mainly the duration from the toe leaving the ground to movement completion. These results suggest that changes in direction may not be perceived precisely by the motor system of the lower limbs, and subjective reaching time was strongly affected by the time after the toe- left the ground. Our findings provide novel insights into the relationship between motor and cognition in multiple directions, which may provide a new strategy for the maximal performance of lower limb movement.
Sit-To-Walk Strategy Classification Using Hip and Knee Joint Angles at Gait Initiation
Authors: Perera Chamalka Kenneth , Gopalai Alpha Agape , Gouwanda Darwin , Ahmad Siti Anom , Salim Mazatulfazura Sf Binti
Published: March 2023

Sit-to-walk (STW) is categorised by three movement strategies - forward continuation, balance and sit-to-stand-and-walk (SiStW). Literature identified strategies through biomechanical parameters using gold standard laboratory equipment, which is expensive, bulky, and not easily integrated into treatment solutions. As strategy becomes apparent at gait-initiation (GI) and the hip/knee are primary contributors in STW, this study proposes the hip/knee joint angles at GI, as an alternate and standalone method of strategy classification - measurable using wearable sensors. To achieve this, K-means clustering was implemented using three clusters and two feature sets (hip/knee angles); with data from an open access online database (age:21–80 years; n = 10). The results identified forward continuation with the lowest hip/knee extension at GI, followed by balance and then SiStW. From this classification, strategy biomechanics were investigated. The biomechanical parameters (derived in this study) that varied between strategies (P < 0.05) were time, horizontal centre of mass (COM) momentum, braking impulse, centre of pressure (COP) range and velocities, COP-COM separation, hip/knee torque and movement fluency. The derived strategy biomechanics are consistent with literature and validate the classification results. Through strategy classification an individual’s strategy-specific biomechanics can be understood and would aid the design and evaluation of interventions for movement impaired individuals.
The Effects of Strong-Weak Swirling Interaction on Emissions in a Multi-Nozzle Combustor
Authors: JIN Ming , Shi Ting , Lu Yudi , Ge Bing , Zang Shusheng
Published: March 2023

Strong-weak swirling interaction has been proved to be effective on combustion instability suppression. A linearly three-nozzle rectangular model combustor is designed to investigate the effects of strong-weak swirling interaction on emissions. Swirl number of swirlers on both sides are 0.72 and that of the middle swirlers are 0.72 and 1.02, respectively. Fuel flow rate remains constant and the equivalence ratio varies from 0.53 to 0.83 by decreasing the air flow rate. High-speed particle imaging velocimetry (HPIV) and RANS methods are applied to study the flow characteristics to reveal the reasons for reduction of pollutants. Results indicate that the "strong-weak" swirling interaction between central and outer swirlers strengthens the turbulence intensity of the swirling flow shear layers, which effectively improve mixing process between fresh premixed gas and burned high-temperature gas to promote combustion characteristics. CO emission varies from 20 to 40 ppm under different equivalence ratios in equal-strength swirl combustor. However, CO emission reduces to below 4 ppm with "strong-weak" swirling interaction. With the increase of swirl number of central swirler, NOx emission remains basically unchanged under the same equivalence ratio. The "strong-weak" swirling interaction also raises the vorticity of main recirculation zone, which strengthens the roll of high-temperature burned gas into the recirculation zone to stabilize flame combustion and widen the operating conditions. In this paper, the equal-strength swirling interaction is converted into a "strong-weak" swirling interaction by increasing the swirling number of central swirler, which helps achieve stable, high-efficiency, low-emission combustion.
Functional consequences of A-to-I editing of miR-379 in prostate cancer cells
Authors: Voss Gjendine , Cassidy James R. , Ceder Yvonne
Published: March 2023

Prostate cancer is the predominant cause of cancer in men, but there is still a lack of biomarkers and treatments for metastatic spread. The initial promise of microRNAs to provide avenues to solve these problems has been dampened by the realisation that microRNAs co-exist in multiple functionally distinct isoforms, for example as a result of A-to-I editing. We recently found that A-to-I-editing of microRNA-379 (miR-379) was associated with prostate cancer, and that only the unedited isoform was negatively correlated with aggressive disease. Here, we set out to decipher the biological effects of unedited and edited miR-379 in prostate cancer cells. After transfection of four different prostate cancer cell lines with isoform-specific miR-379 mimics we performed assays for cell growth, colony formation, migration, cell-cell adhesion and epithelial-mesenchymal transition (EMT) and stemness markers. We found that unedited miR-379 had an effect on cell growth, with a promoting function in androgen receptor (AR)-negative cells and an inhibiting effect in AR-positive cells. We further found that both miR-379 isoforms could promote colony formation, migration and cell-cell adhesion. Overall, unedited miR-379 had a more pronounced biological function in prostate cancer cells, and miR-379 editing may primarily serve to downregulate the function of unedited miR-379.
Identification of Novel Lung Adenocarcinoma Subtypes and Prognostic Gene Sets Based on GSVA Analysis
Authors: Yuan Jianxu , Wang Jiawu , Jiang Qing , Hua Zhengzhao , Yu Shengjie
Published: March 2023

Background
Lung cancer is one of the most common malignant tumors of the respiratory system in the world. According to the traditional histological classification, it can be divided into many subtypes. In recent years, the incidence rate of lung adenocarcinoma (LUAD) has been rising rapidly. In this study, we identified the biomarkers related to the prognosis of LUAD through the protein-protein interaction (PPI) network analysis, gene set variation analysis (GSVA) and the "CancerSubtypes" software package in R, with a view to having a positive impact on the future treatment and new drug development.
Method
We obtained the relevant data needed for the study from The Cancer Genome Atlas (TCGA) database and the GEO database. Through GSVA analysis, the gene expression matrix was further transformed into the gene set expression matrix for subsequent research. Then, we applied the package "CancerSubtypes" in R to transform the samples into different subtypes, and established a LUAD-related prognosis model based on the differential expression gene sets (DEGSs) among the subtypes. Finally, we performed functional and pathway enrichment analysis together with PPI network analysis of genes from prognosis related DEGSs.
Results
A total of 600 LUAD samples were obtained from TCGA database, including 541 tumor samples and 59 normal samples. We screened 507 tumor samples for further classification, including 166 cases of subtype 1, 138 cases of subtype 2, and 203 cases of subtype 3. Subsequently, we identified 63 DEGSs and constructed the prognostic characteristics of LUAD with 4 of them (“T_GSE24634_TREG_VS_TCONV_POST_DAY5_IL4_CONVERSION_UP” “T_GSE25088_WT_VS_STAT6_KO_MACROPHAGE_DN” “T_GSE45365_HEALTHY_VS_MCMV_INFECTION_CD11B_DC_DN” and “T_HALLMARK_MYC_TARGETS_V2”). Finally, we established the corresponding PPI network with 6 subnets, and identified 15 core proteins including CCNB2, KIF2C, TPX2, PES1, BRIX1, NIP7, PSMB4, PSMD12, PSMC3, MPHOSPH10, WDR43, POLA1, MCM4, PAICS and GART.
Conclusions
In this study, we identified four gene sets related to the prognosis of LUAD and obtained 15 core proteins. This study could provide relevant theoretical basis and guidance for the update of treatment methods and the development of new drugs, related to LUAD and other cancers.
Efficient and ultrafast organic scintillators by hot exciton manipulation
Authors: Tang Jiang , Du Xinyuan , Zhao Shan , Wang Lu , Wu Haodi , Ye Fan , Xue Kan-Hao , Peng Shaoqian , Xia Jianlong , Sang Ziru , Zhang Dongdong , Xiong Zuping , Zheng Zhiping , Xu Ling , Niu Guangda
Published: March 2023

Efficient and fast scintillators are in high demand for a variety of fields, such as medical diagnostics, scientific instruments, and high-energy physics. However, the trade-off between high scintillation efficiency and fast timing properties is a common challenge faced by almost all scintillators. To overcome this limitation, we have developed a new strategy for organic scintillators by directing all hot excitons into fast singlet emission states without involving the lowest triplet states. Our scintillator 1,1,2,2-tetraphenylethylene (4-bromophenyl) (TPE-4Br) shows an ultrafast radiative lifetime of 1.79 ns and ~ 72600 photons MeV− 1 light yield, exhibiting an unprecedented combination of high light yield and short decay time. Our work provides a paradigm-shifting method to design efficient and ultrafast scintillators and paves the way towards exciting applications toward ultrafast detection and imaging.
Trajectories of children and adolescents attending a psychiatric emergency unit during the COVID-19 confinements: 2020-2022 longitudinal study
Authors: Adrados-Pérez Marina , Llorca-Bofí Vicent , Mur-Laín María , Albert-Porcar Carla , Nicolau-Subires Eugènia , Ibarra-Pertusa Lucía , Jiménez-Mayoral Andrea , Buil-Reiné Esther , Budny Filip , Resa-Pérez Belén , Velásquez-Acebey Vanessa Gladys , Arenas-Pijoan Laura , Irigoyen-Otiñano María , López-Castroman Jorge
Published: March 2023

Introduction: The prevalence of psychiatric disorders has not shifted widely through the COVID pandemic, except for some specific groups such as young people or women. Our objective is to examine prospectively the evolution of children and adolescents who consulted in a psychiatric emergency service during the COVID-19 confinements.
Method: We collected prospective clinical information about 296 young people under 18 who visited a tertiary hospital for psychiatric reasons during the confinement periods in Spain. Clinical diagnoses, suicide attempts, hospital admissions, and pharmacological prescriptions were extracted from electronic health records through 2020, 2021, and 2022. Features of those who maintained psychiatric care and those who did not were compared.
Results: Three out of four children and adolescents who visited the psychiatric emergency department during the confinements continued psychiatric care at the end of 2022. Those who did not showed better premorbid adjustment at baseline. During follow-up, diagnoses of neurodevelopmental disorders and eating disorders, as well as the dosage of psychotropic drug prescriptions, increased. The diagnoses of major depressive disorder and eating disorder at baseline were associated with attempting suicide during follow-up. Patients with internalizing symptoms were admitted earlier than those with externalizing symptoms but no differences were found in terms of suicide attempts.
Conclusions: The continuity of psychiatric care after an initial emergency visit during the confinements implied greater clinical severity, as reflected by changes in clinical diagnoses and pharmacological regimens. Emergent symptoms of depression or eating disorders after social distancing or isolation could predict subsequent suicidal behavior in young populations.
A novel approach for improving the accuracy using word embedding on deep neural networks for software requirements classification
Authors: P Saratha , Mukherjee Saswati
Published: March 2023

Software requirements classification is essential for later use in the design and implementation phases. Traditional manual classification methods have been unable to keep up with the massive volume of current data. Automatic requirement classification is a crucial and promising field in industry and academia. Deep learning technology speeds up the advancement of requirements classification. Moreover, word embedding enables the representation of words learned from a corpus as vectors that map words with similar meanings to have similar presentations. We demonstrated few deep learning models, such as Convolutional Neural Networks (CNN), Long Short Term Memory (LSTM), and Bi-directional Long Short Term Memory (BiLSTM), to classify the requirements based on the word embedding methods Word2Vec and Global Vectors (GloVe). We deal with requirements categorization as binary, multi-class, and multi-label classification, even though a particular need may belong to numerous classes simultaneously. Then, we analyze the performance of the embedding methods, their impacts on the accuracy rate, and the effectiveness of the deep neural network architectures in detail. We have experimented on the publicly accessible Predictor Models in Software Engineering (PROMISE) and Electronic Health Records (EHR) datasets to demonstrate the efficacy of deep learning models. This research aims to classify requirements into functional and non-functional categories with minimal pre-processing. Also, it indicates that deep learning is superior for analyzing requirements classification and suggests possibilities for future work.
Construction and validation of an anoikis-related gene prognostic signature in ovarian cancer
Authors: Tang Zhenye , Huang Shuting , Liu Dajiang , Liu Yueyang , Chen Jing , Zhou Chenfei , He Shanyang
Published: March 2023

Ovarian cancer (OC) is identified as one of the most aggressive malignant diseases that imperil women health. Anoikis has been proved to be significantly correlated with the aggressiveness of dislodged cancer cells and poor prognosis of cancer patients. Currently, few researches focus on the prognostic role of anoikis-related genes (AIRGs) on the OC cases. Here, we firstly and systematically analyzed the predictive ability of AIRGS on the prognosis of patients with OC. The genomics data and clinicopathologic information of OC patients were retrieved from TCGA project. Univariate Cox regression and least absolute shrinkage and selection operator (LASSO) method were executed to construct an anoikis–related risk score (AIRS) indicator based on 11 AIRGs. ICGC-OV and GSE26712 were used as independent validation datasets. A nomogram integrated AIRS and pathologic parameters, survival analysis and time-dependent receiver operating characteristic (ROC) curves were utilized to assess the predictive performance of this signature. Successfully, OC patients were divided into two sets based on the optimum cut-off value of risk scores. High risk category evidently suffers from more unfavorable survival outcomes. What is more, AIRS was closely connected with tumor microenvironment (TME) characteristics, mutant landscape, m6A modification and clinical implications. Interestingly, we validated EML2 as a crucial risk gene in OC based on GSEA, single-cell analysis and immunohistochemical staining results. Collectively, AIRS was validated as an independent prognostic indicator with good predictive validity in OC. It can function as a robust clinical parameter which effectively stratifies OC patients and promotes the development of precise medicine.
Relationship between disgust and Orthorexia Nervosa and psychometrics properties of the Italian Dusseldorf Orthorexia Scale in a general population sample
Authors: Aloi Matteo , Moniaci Martina , Rania Marianna , Carbone Elvira Anna , Martino Gabriella , Segura-Garcia Cristina , Liuzza Marco Tullio
Published: March 2023

Background
It remains unclear among clinicians and researchers whether Orthorexia Nervosa (ON) is a part of the obsessive-compulsive disorder (OCD) spectrum or eating disorders (EDs). Disgust seems to be a shared psychopathological factor in these clinical presentations, indicating a potentially crucial role in ON. On the other hand, numerous psychometric tools have been developed to evaluate ON. The Dusseldorf Orthorexia Scale (DOS) was recently validated in an Italian sample. However, the study's primary limitation was that the scale was only administered to undergraduate university students. This study aimed to investigate the psychometric properties (including factorial structure, reliability, and measurement invariance conditional on sex) of the Italian version of the DOS (I-DOS) on a sample from the general population. Additionally, the study sought to determine the nomological validity of the I-DOS by examining its relationship with disgust sensitivity.
Methods
A sample of 521 participants took part in this study and completed a battery that assessed ON and disgust sensitivity. To assess the I-DOS structure, reliability, and measurement invariance we respectively conducted confirmatory factor analysis (CFA), computed McDonalds’s omega, and performed hierarchical series of multigroup CFAs. Then, we tested the relationship between ON and disgust sensitivity.
Results
CFA confirmed the unifactorial model of I-DOS and it respected the configural, metric, and strict invariance while a partial scalar invariance was achieved. It also showed good reliability with an omega of 0.87. In addition, we found a positive relationship between ON and disgust sensitivity, thus confirming the nomological validity of I-DOS.
Conclusions
Our findings suggest that the Italian version of the Dusseldorf Orthorexia Scale (I-DOS) exhibits strong psychometric properties and can be an effective instrument for assessing ON in a general population sample. Notably, the most significant and innovative outcome was the positive correlation between ON and disgust sensitivity. As disgust has been linked to other clinical presentations, this preliminary result could serve as a foundation for future research exploring this phenomenon in greater detail.
Transcriptomic analysis reveals novel age-independent immunomodulatory proteins as a mode of cerebroprotection in P2X4R KO mice after ischemic stroke
Authors: Gamiotea-Turro Daylin , Cronin Chunxia C , Liang Bruce T , Verma Rajkumar
Published: March 2023

Identification of new potential drug target proteins and their plausible mechanisms for stroke treatment is critically needed. We previously showed that genetic deletion and short-term pharmacological inhibition of P2X4R, a purinergic receptor for adenosine triphosphate ATP, provides acute cerebroprotection. However, potential mechanisms remain unknown. Therefore, we employed RNA-seq technology to identify the gene expression profiles, pathway analysis, and qPCR validation of differentially expressed genes (DEGs). This analysis identified roles of DEGs in certain biological processes responsible for P2X4R-dependent cerebroprotection after stroke. We subjected both young and aged male and female global P2X4 KO and littermate WT mice to ischemic stroke. After 3 days, mice were sacrificed, total RNA was isolated using Trizol, and subjected to RNA-seq and Nanostring-mediated qPCR. DESeq2, Gene Ontology (GO), and Ingenuity Pathway Analysis (IPA) were used to identify mRNA transcript expression profiles and biological pathways. We found 2246 DEGs in P2X4R KO vs WT tissue after stroke. Out of these DEGs, 1920 gene were downregulated, and 325 genes were upregulated in KO. GO/IPA analysis of the top 300 DEGs suggests an enrichment of inflammation and extracellular matrix component genes. qPCR validation of the top 30 DEGs revealed downregulation of two common age-independent genes in P2X4R KO mice: Interleukin-6 (IL-6), an inflammatory cytokine, and Cytotoxic T Lymphocyte-Associated Protein 2 alpha (Ctla2a), an immunosuppressive factor. These data suggest that P2X4R-mediated cerebroprotection after stroke is initiated by attenuation of immune modulatory pathways in both young and aged mice of both sexes.
Metabolism and Tumor Microenvironment-Associated Molecular Classification of Neuroblastoma
Authors: Du Xin , Jiang Shayi , Yang Jingwei , Liao XueLian , Zou Bing , Shao Jingbo
Published: March 2023

Neuroblastoma (NBL) is clinically and biologically heterogeneous, and novel therapies are desperately needed as the poor prognosis of high-risk NBL cases. Increasingly, studies about metabolic reprogramming and tumor microenvironment (TME) open the way to change cancer risk stratification and treatment. Through machine learning, this study identified two metabolic clusters in NBL, which have distinct clinical features, Hallmark pathways and TME. By Weighted gene co-expression network analysis (WGCNA) and Cytoscape, we discovered that RNA and glycosphingolipid (GSL) metabolism play a crucial role in metabolic subtyping. Subsequently, we constructed and verified a risk signature based on key module genes, which performs a good prediction of NBL prognosis. Two risk groups, divided by the median value, are closely associated with clinical features, and risk scores show an inverse correlation with immune infiltration. In combination with gene set enrichment analysis (GSEA) results, we conclude that ribosome biogenesis regulated by rRNA metabolism might be a target for MYCN-amplification NBLs, and GSL metabolism might contribute to TME formation in NBL. Finally, we tried to predict different risk groups' immunotherapy and chemotherapy sensitivity and screened potential targets that might be useful against NBL. In summary, we used multiple bioinformatics analyses to explore the interaction between metabolic processes and TME in NBL and provide new ideas for developing new therapies.
Weak congruence between biological assemblages of streams and their relationship with the environmental gradient in the Cerrado-Amazon transition area, Brazil
Authors: Alexandre Rafaela Jemely Rodrigues , Montag Luciano Fogaça de Assis , Dias-Silva Karina , Brasil Leandro Schlemmer , Maracahipes-Santos Leonardo , Batista Joana Darc , Vieira Thiago Bernardi
Published: March 2023

Congruence between taxa is the agreement between groups, it is used to find indicator taxa, being a quick and effective way to assess biodiversity. The objective of this work was to evaluate the congruence between fish assemblages, Heteroptera, Odonata in streams of the Amazon-Cerrado transition area. The study took place in 24 streams in the transition between the Cerrado and Amazon biomes, Brazil. The analysis results showed weak congruence between Odonata and Heteroptera for the two data sets. Disagree Odonata and fish showed weak congruence but signicant with abundance data, and with incidence data, the congruence was not significant. A similar result was obtained when analyzing fish and Heteroptera, which were not congruent with any of the datasets used. These findings were repeated when analyzing the suborder Zygoptera separately, instead of Odonata. The variance partition test, with abundance data, showed that both environmental variables and space are responsible for structuring the Heteroptera community, different fish, Odonata and Zygoptera did not respond to any of the analyzed variables, with incidence data no group showed a relationship with the environmental and spatial variables analyzed. The pRDA, with abundance data, confirmed the relationship of the Heteroptera assemblage with environmental and spatial variables, however, the other assemblages did not present significant results. With the incidence data, it was not significant for all assemblages. The weak congruence between the different groups can due to the different responses to the same environmental gradient, which was confirmed by the variance partition test.
A novel t (5; 17) (q35; q21) associated with t (8; 21) (q22; q22) in a patient with acute myeloid leukemia: Case report and review of literature
Authors: Zahra Kmira , Cherif Wided , Ahmed Gereisha , Regaieg Haifa , Nesrine Ben Sayed , Zaier Monia , Mootamri Wided , Youssef Yosra Ben , Brahem Nejia , Sennana Halima , Khelif Abderrahim
Published: March 2023

The t (8; 21) (q22; q22) with the resulting RUNX1- RUNX1T1 rearrangement is one of the most common cytogenetic abnormalities in acute myeloid leukemia (AML). It is associated with favorable prognosis. The t (5; 17) (q35; q21) is an uncommon translocation, fuses the gene for the nucleophosmin (NPM) to the retinoic acid receptor α(RARA) and was described essentially in acute promyelocytic leukemia (APL) variant. We present the case of a 19-year-old male patient who developed an AML with t (8 ;21) (q22 ;q22) associated to t(5 ;17)(q35 ;21). Morphology and immunophenotype of the leukemic cells were compatible with AML. The patient received chemotherapy based on cytarabine and anthracycline without all-trans retinoic acid (ATRA) followed by allogenic stem cells transplantation in first remission. To the best of our knowledge, this is the first report of an association between a rare translocation t (5; 17) and t (8;21) in AML. In this report, we will discuss the prognosis of this association as well as the treatment.
The Impact of Body Mass Index and Medical Conditions on Home-based Anal Self-Sampling
Authors: Nitkowski Jenna , Fernandez Maria E. , Ridolfi Tim , Chiao Elizabeth , Giuliano Anna R. , Schick Vanessa , Swartz Michael D. , Smith Jennifer S. , Nyitray Alan G.
Published: March 2023

Purpose. Self-sampling is increasingly being used in screening programs, yet no studies to date have examined the impact of bodily characteristics on self-sampling experiences. Our objective was to assess whether body mass index (BMI) and physical disability were associated with anal self-sampling difficulty.
Methods.We recruited sexual minority men (SMM) and trans persons in Milwaukee, Wisconsin to participate in an anal cancer screening study. Between January 2020 and August 2022, 240 participants were randomized to a home (n=120) or clinic (n=120) screening arm. Home participants received a mailed at-home anal self-sampling kit and were asked to attend a baseline clinic visit where biometric measurements were collected. Participants were asked to complete a survey about their experience with the kit. This research utilizes data from participants who used the kit and completed a baseline clinic visit and post-swab survey (n=82). We assessed the impact of BMI and physical disability on reported body or swab positioning difficulty.
Results. Most participants reported no or little difficulty with body positioning (90.3%) or swab positioning (82.9%). Higher BMI was significantly associated with greater reported difficulty with body positioning (aOR=1.10, 95% CI 1.003-1.20, p=.04) and swab positioning (aOR=1.11, 95% CI 1.02-1.20, p=.01). Physical disability was not significantly associated with body or swab positioning difficulty. Specimen adequacy did not differ by BMI category (p=.76) or physical disability (p=.88).
Conclusion. Anal self-sampling may be a viable option to reach obese persons who may be more likely to avoid screening due to weight-related barriers.
Bivariate Sin-Generalized Exponential Model with Applications on Medicine, Economic, and Environmental Data
Authors: Hassanein W. A. , Sobhy A. M.
Published: March 2023

This paper focuses on answering the question; How we can construct a bivariate model with fewer parameters that serve many disciplines in lifetime data? The answer to this question is introduced in this paper by proposing a new bivariate model that depends on constructing a SinTransformation of its marginals in the Farlie-Gumbel Morgenstern (FGM) Copula Model. This bivariate model is named by Morgenstern Sin Generalized Exponential (MSGE) Distribution. Without adding any extra parameter, MSGE provides a statistical model which gives better results in the goodness of fit to medical, economic, and environmental real lifetime data compared with other well-known models. The mathematical procedure of MSGE is carried out for statistical and reliability properties, estimation via 3 methods, simulation, and real practical applications in the three mentioned disciplines. The results illuminate the effectiveness of MSGE for the practitioner without overloading extra parameters.
Exosome-derived circKIF20B suppresses gefitinib resistance and cell proliferation in non-small cell lung cancer
Authors: Wei Si-Liang , Ye Jing-Jing , Sun Li , Hu Lei , Wei Yuan-Yuan , Zhang Da-Wei , Xu Meng-Meng , Fei Guang-He
Published: March 2023

Background The gefitinib resistance mechanism in non-small cell lung cancer (NSCLC) remains unclear, albeit exosomal circular RNA (circRNA) is known to possiblyplay a vital role in it.
Methods We employed high-throughput sequencing techniques to detect the expressions of exosomalcircRNA both in gefitinib-resistant and -sensitive cells in this study. The circKIF20B expression was determined in serum exosomes and tissues of patients by qRT-PCR. The structure, stability, and intracellular localization of circKIF20B were verified by Sanger sequencing, Ribonuclease R (RNase R)/actinomycin D (ACTD)treatments, and Fluorescence in situ hybridization (FISH). The functions of circKIF20B were investigated by 5-Ethynyl-20-deoxyuridine (EdU), flow cytometry, Cell Counting Kit-8 (CCK-8), oxygen consumption rate (OCR), and xenograft model. Co-culture experiments were performed to explore the potential ability of exosomal circKIF20B in treatinggefitinib resistance. The downstream targets of circKIF20B were determined by luciferase assay, RNA pulldown, and RNA immunoprecipitation (RIP).
Results We found that circKIF20B was poorlyexpressed in the serum exosomes of gefitinib-resistant patients (n = 24) and the tumor tissues of patients with NSCLC (n = 85). CircKIF20B was negatively correlated with tumor size and tumor stage. Decreasing circKIF20B was found to promote gefitinib resistance by accelerating the cell cycle, inhibiting apoptosis, and enhancing mitochondrial oxidative phosphorylation (OXPHOS), whereas increasing circKIF20B was found to restore gefitinib sensitivity. Mechanistically, circKIF20B bound to miR-615-3p for regulating the MEF2A and then altering the cell cycle, apoptosis, and mitochondrial OXPHOS. Overexpressing circKIF20B parental cells can restore sensitivity to gefitinib in the recipient cells by upregulating the exosomal circKIF20B expression.
Conclusions This study revealed a novel mechanism of circKIF20B/miR-615-3p/MEF2A signaling axis involving progression of gefitinib resistance in NSCLC. Exosomal circKIF20B is expected to be an easily accessible and alternative liquid biopsy candidate and potential therapeutic target in gefitinib-resistant NSCLC.
Effects of fiber content and layer thickness on the final crack morphology of fine-grained coral soil by desiccation cracking tests
Authors: Ding Xuan-Ming , Fang Hua-Qiang , Ou Qiang , Jiang Chun-Yong , Li Yifu
Published: March 2023

Purpose
Fine-grained coral soil as a special kind of marine soil, its potential application value has become increasingly prominent with the strategic goal of 'building a maritime power' proposed. It is necessary to study the modification of fine-grained coral soil to improve its crack resistance, so as to be better applied to practical engineering.
Materials and methods
The effects of PVA fiber (polyvinyl alcohol fiber) content and layer thickness on the final crack morphology of fine-grained coral soil were studied. Through the desiccation crack test, the final crack parameters, clod parameters, fractal dimension and crack morphology distribution of fine-grained coral soil were obtained by using image processing technology, and the quantitative analysis was carried out. Finally, the final crack morphology of fine-grained coral soil under the coupling effect of fiber content and layer thickness was obtained.
Results and discussion
The increase of layer thickness can reduce the number of nodes and number of crack segments, the total crack length and the fractal dimension of crack length. However, it can lead to the increase of average crack width, average crack length, total crack area, surface crack rate and surface crack ratio. The relative frequency distribution ranges of crack length and crack width are also gradually expanding. Moreover, the frequency distribution of crack length and crack width of fine-grained coral soil conforms to the Lognormal function. When the fiber content is low or no fiber is added, the frequency distribution of clod area also conforms to the Lognormal function.
Conclusions
Our study suggested that the crack resistance of fine-grained coral soil is improved with the increase of fiber content, which effectively inhibits the extension of cracks along the length direction and the expansion of width direction. In addition, The layer thickness also has a significant effect on the soil desiccation cracks. Revealing its law is of great significance for the potential application of fine-grained coral soil.