bluuebunny/crossref_metadata_2025 huggingface dataset:
Advances in Boundary Value Problems for Fractional Differential Equations
Authors: Luca Rodica
Published: May 2023

Fractional-order differential and integral operators and fractional differential equations have extensive applications in the mathematical modelling of real-world phenomena which occur in scientific and engineering disciplines such as physics, chemistry, biophysics, biology, medical sciences, financial economics, ecology, bioengineering, control theory, signal and image processing, aerodynamics, transport dynamics, thermodynamics, viscoelasticity, hydrology, statistical mechanics, electromagnetics, astrophysics, cosmology, and rheology [...]
P-Bifurcation Analysis for a Fractional Damping Stochastic Nonlinear Equation with Gaussian White Noise
Authors: Tang Yujie , Peng Yun , He Guitian , Liang Wenjie , Zhang Weiting
Published: May 2023

This work aims to address the P-bifurcation of a stochastic nonlinear system with fractional damping driven by Gaussian white noise. Based on a stochastic averaging method, a fractional damping stochastic nonlinear equation has been studied. Furthermore, the expressions of drift and diffusion coefficients of the Fokker–Planck equation (FPKE) have been obtained. The probability density function (PDF), the steady solution of FPKE, has also been derived. Then, PDFs of two fractional damping Morse oscillators have been obtained. One can note that the analytical results coincide with the results of numerical simulation. Importantly, stochastic P-bifurcation of a fractional damping stochastic nonlinear Morse oscillator has been further addressed and analyzed.
The Impact of Telemedicine on Patients with Hidradenitis Suppurativa in the COVID-19 Era
Authors: Gierek Marcin , Kitala Diana , Łabuś Wojciech , Glik Justyna , Szyluk Karol , Pietrauszka Kornelia , Bergler-Czop Beata , Niemiec Paweł
Published: May 2023

(1) Background: Hidradenitis suppurativa is a chronic, inflammatory skin disease. It is characterized by the transformation of normal skin into skin with abscesses, nodules, tunnels, and scars. The most commonly affected areas are the armpits, groins, buttocks, and subscapular area. Patients with HS require constant care under the supervision of the outpatient clinic. Due to the COVID-19 pandemic, consultations have been introduced in the form of telemedicine. The aim of this study was to evaluate the availability of HS treatment during the COVID-19 pandemic and to assess patient satisfaction, problems with access to medical care, and the impact of the pandemic on the course of the disease. (2) Methods: An internet survey with an anonymous questionnaire was used to assess the effectiveness of telemedicine consultations. The survey consisted of 25 closed questions, and responses were kept fully anonymous. (3) Results: Most respondents reported minor problems with accessing specialized HS medical care during the COVID-19 pandemic (n = 25, 35.71%). However, 35.71% (n = 25) of them reported major problems with appointments for specialized ambulatory treatment during the last few months of the pandemic, mainly due to delayed appointments. Almost half of the respondents had been diagnosed with COVID-19 (n = 34, 48.57%), and 58.57% (n = 41) of respondents did not see a correlation between COVID-19 infection and HS progression. (4) Conclusions: Our study showed that the pandemic significantly limited access to medical advice, and patients with hidradenitis suppurativa prefer standard consultations.
Trends in Health Communication: Social Media Needs and Quality of Life among Older Adults in Malaysia
Authors: Chen Hana W. Jun , Marzo Roy Rillera , Sapa Nur Hafizah , Ahmad Absar , Anuar Haryati , Baobaid Mohammed Faez , Jamaludin Nurul Akmal , Hamzah Hazian , Sarrafan Siamak , Ads Hassan Omar , Kumar Kavitha Ashok , Hadi Jalal , Sazali Hafsah , Abdalqader Mohammed A.
Published: May 2023

Background: While social media continues to dominate, social media platforms have become powerful health communication tools for older users. However, fulfilling their social media needs can be both detrimental and beneficial to their quality of life (QoL). This study assessed social media needs as they relate to QoL among older adults in Malaysia. Methods: We conducted a cross-sectional study and adopted convenience sampling to recruit participants. The participants were required to self-report their sociodemographic profile, social media use and needs, and QoL. Social media use and needs were assessed using the Social Networking Sites Uses and Needs (SNSUN) scale, and QoL was assessed using the WHOQOL-BREF questionnaire. Multiple linear regression was performed to identify the predictors of QoL. Results: The findings revealed that the fulfilment of social integrative needs was the strongest predictor of higher QoL in all domains. However, those using social media for their affective needs demonstrated lower psychological health quality. Conclusions: Fulfilling social integrative needs is the key to improving the QoL among older adults. The continuous development of age-friendly applications is essential to keep up with constantly changing social media trends and bridge the gap of social media inequalities. More importantly, it would enable older adults to utilize social media to its fullest potential and enjoy a higher QoL through accessible health communication tools.
Herbal Medicine Yukgunja-Tang for Functional Dyspepsia: A Protocol for a Randomized, Controlled, Multicenter Clinical Trial
Authors: Lee Boram , Ha Na-Yeon , Park Hyo-Ju , Kim Ae-Ran , Kwon O-Jin , Cho Jung-Hyo , Shin Seon Mi , Kim Jinsung , Yang Changsop
Published: May 2023

The herbal medicine Yukgunja-tang has been widely used for the treatment of functional dyspepsia (FD) in the clinical setting of East Asian traditional medicine. This paper presents a protocol for a randomized, assessor-blind, controlled, multicenter, three-arm parallel clinical trial comparing the effectiveness, safety, and cost-effectiveness of Yukgunja-tang with Pyeongwi-san and usual care. A total of 140 participants with Rome IV-diagnosed FD will be randomly assigned to either the Yukgunja-tang (n = 56), Pyeongwi-san (n = 56), or usual care (n = 28) groups. All participants will be educated on dietary guidelines for FD patients. Participants in the Yukgunja-tang and Pyeongwi-san groups will take investigational products for 6 weeks. All participants will be assessed for clinical parameters at weeks 0, 3, 6, 9, and 24. The primary outcome will be measured on the total dyspepsia symptom scale, and the secondary outcome will include the single dyspepsia symptom scale, overall treatment effect, the visual analog scale for dyspepsia, FD-related quality of life, hospital anxiety and depression scale, EuroQol-5 dimension, pattern identification, and serum levels of acyl-ghrelin and deacyl-ghrelin. Adverse events and laboratory tests will be monitored for safety assessment. The results will provide evidence of the effectiveness, safety, and cost-effectiveness of Yukgunja-tang in the treatment of FD.
Assisted Suicide and Euthanasia in Mental Disorders: Ethical Positions in the Debate between Proportionality, Dignity, and the Right to Die
Authors: Scopetti Matteo , Morena Donato , Padovano Martina , Manetti Federico , Di Fazio Nicola , Delogu Giuseppe , Ferracuti Stefano , Frati Paola , Fineschi Vittorio
Published: May 2023

The admission of people suffering from psychiatric and neurocognitive disorders to euthanasia and physician-assisted suicide (E/PAS) in some European and non-European countries represents a controversial issue. In some countries, the initial limitation of E/PAS to cases of severe physical illness with poor prognosis in the short term has been overcome, as it was considered discriminatory; thus, E/PAS has also been made available to subjects suffering from mental disorders. This decision has raised significant ethical questions regarding the capacity and freedom of self-determination; the family, social, and economic contexts; the social consideration of the sense of dignity and the pressure on the judgment of one’s personal value; the contextual therapeutic possibilities; the identification of figures involved in the validation and application; as well as the epistemological definitions of the clinical conditions in question. To these issues must be added the situation of legislative vacuum peculiar to different countries and the widespread lack of effective evaluation and control systems. Nonetheless, pessimistic indicators on global health status, availability of care and assistance, aging demographics, and socioeconomic levels suggest that there may be further pressure toward the expansion of such requests. The present paper aims to trace an international overview with the aim of providing ethical support to the debate on the matter. Precisely, the goal is the delimitation of foundations for clinical practice in the complex field of psychiatry between the recognition of the irreversibility of the disease, assessment of the state of physical and mental suffering, as well as the possibility of adopting free and informed choices.
Synergistic Effect of a Plant-Derived Protein Hydrolysate and Arbuscular Mycorrhizal Fungi on Eggplant Grown in Open Fields: A Two-Year Study
Authors: Di Miceli Giuseppe , Vultaggio Lorena , Sabatino Leo , De Pasquale Claudio , La Bella Salvatore , Consentino Beppe Benedetto
Published: May 2023

Plant biostimulants, such as plant protein hydrolysates (PHs) and arbuscular mycorrhizal fungi (AM), are natural products capable of increasing the yield and quality of crops and decreasing the ecological impact of plant growing cycles. However, there is little research on the mutual application of different categories of biostimulants (microbial and non-microbial). The current study was conducted to examine the effects of “Trainer” PH application (0 or 3 mL L−1) and AM (R. irregularis) inoculation on the growth, yield, quality and nitrogen indices of ”Birgah” F1 eggplant cultivated for two years (2020 and 2021). Results revealed that the combined application of PH and AM significantly enhanced total and marketable yields, average marketable fruit weight and number of marketable fruits by 23.7%, 36.4%, 19.0% and 11.1% compared to non-treated plants (control), respectively. Moreover, biostimulants increased the soluble solids content (SSC), chlorogenic acid, total anthocyanins, K and Mg in the fruits by 16%, 4.6%, 6.4%, 8.6% and 23.9% compared to control plants, respectively. Interestingly, the mutual application of PH and AM improved fruit quality by reducing the glycoalkaloid concentration (−19.8%) and fruit browning potential (−38%). Furthermore, both biostimulants exerted a synergistic action, enhancing nitrogen use efficiency and nitrogen uptake efficiency by 26.7% and 18.75%, respectively. On the other hand, productive and fruit-quality features were significantly influenced by the year due to remarkable differences in terms of maximum temperature between the first and second cultivation cycles. Overall, our research underlined that PH and AM can positively interact to improve the performance of eggplant cultivated in open fields.
The Concept of “Hypersexuality” in the Boundary between Physiological and Pathological Sexuality
Authors: Perrotta Giulio
Published: May 2023

Introduction: The concept of hypersexuality belongs to modern parlance, according to a predominantly clinical meaning, and is understood as a psychological and behavioural alteration as a result of which sexually motivated stimuli are sought in inappropriate ways and often experienced in a way that is not completely satisfactory. Methods: Literature up to February 2023 was reviewed, with 25 searches selected. Results: Forty-two articles were included in the review. Conclusion: Hypersexuality is a potentially clinically relevant condition consisting of one or more dysfunctional and pathological behaviours of one’s sexual sphere and graded according to the severity of impairment of subjective acting out; for this reason, the Perrotta Hypersexuality Global Spectrum of Gradation (PH-GSS) is suggested, which distinguishes high-functioning forms (pro-active and dynamic hypersexuality) from those of attenuated and corrupted functioning (dysfunctional and pathological hypersexuality of grades I and II). Future research is hoped to address the practical needs of this condition, such as the exact etiopathology, the role of oxytocin in dopaminergic hypotheses (and its ability to attenuate the symptomatology suffered by the patient in terms of manic drive), the best structural and functional personality framing of the subject, and the appropriate therapy to pursue.
Exploring the Relationship between the Gut Microbiota and Ageing: A Possible Age Modulator
Authors: Salazar Juan , Durán Pablo , Díaz María P. , Chacín Maricarmen , Santeliz Raquel , Mengual Edgardo , Gutiérrez Emma , León Xavier , Díaz Andrea , Bernal Marycarlota , Escalona Daniel , Hernández Luis Alberto Parra , Bermúdez Valmore
Published: May 2023

The gut microbiota (GM) has been the subject of intense research in recent years. Therefore, numerous factors affecting its composition have been thoroughly examined, and with them, their function and role in the individual’s systems. The gut microbiota’s taxonomical composition dramatically impacts older adults’ health status. In this regard, it could either extend their life expectancy via the modulation of metabolic processes and the immune system or, in the case of dysbiosis, predispose them to age-related diseases, including bowel inflammatory and musculoskeletal diseases and metabolic and neurological disorders. In general, the microbiome of the elderly tends to present taxonomic and functional changes, which can function as a target to modulate the microbiota and improve the health of this population. The GM of centenarians is unique, with the faculty-promoting metabolic pathways capable of preventing and counteracting the different processes associated with age-related diseases. The molecular mechanisms by which the microbiota can exhibit anti-ageing properties are mainly based on anti-inflammatory and antioxidant actions. This review focuses on analysing the current knowledge of gut microbiota characteristics and modifiers, its relationship with ageing, and the GM-modulating approaches to increase life expectancy.
Age Friendly Characteristics and Sense of Community of an Italian City: The Case of Macerata
Authors: Monachesi Paola
Published: May 2023

The paper presents a study about the city of Macerata, as a representative case of an urban community in the Marche Region, Italy. The aim of this paper is to assess the level of its age-friendliness by means of a quantitative analysis based on a questionnaire that relies on the well-established eight AFC domains proposed by the WHO. In addition, the sense of community (SOC) is investigated and how the older residents relate to it. Studies that analyze age-friendly Italian cities in relation to elder outcomes are limited. The paper contributes to fill this gap, and the findings reveal that the elderly respondents are not particularly satisfied about the services and the urban infrastructure of the city but show nevertheless a sense of community. It might be the combination of urban and rural features that contributes to the longevity and strong sense of community of the city despite its poor infrastructure and average services.
Negative Interest Rates and Its Impact on GDP, FDI and Banks’ Financial Performance: The Cases of Switzerland and Sweden
Authors: Wawrosz Petr , Traksel Semen
Published: May 2023

The article deals with the issue of how negative interest rate policies, introduced in the second decade of the 21st century in some countries, affect certain macroeconomic indicators and bank performance. We concentrate specifically on Switzerland and Sweden. We use correlation analysis to reveal the relationship between interest rates and GDP, the level of foreign direct investment (FDI) and some indicators of banks’ performance. We found that negative interest rates (NIRs) are strongly correlated with the level of GDP in both Switzerland and Sweden but that they do not affect their FDI. The share of banks´ deposits in GDP is also strongly correlated with NIR. Other indicators of bank performance do not show a strong correlation for both countries. Our evidence is consistent with NIR not being associated with undesirable effects concerning economic growth and bank performance in Switzerland and Sweden. The value of FDI depends on many factors—mainly on the attractiveness of a country for foreign investors in terms of its political and economic stability and by general conditions for business operation.
Quantifying the Effect of Socio-Economic Predictors and the Built Environment on Mental Health Events in Little Rock, AR
Authors: Ek Alfieri , Drawve Grant , Robinson Samantha , Datta Jyotishka
Published: May 2023

Law enforcement agencies continue to grow in the use of spatial analysis to assist in identifying patterns of outcomes. Despite the critical nature of proper resource allocation for mental health incidents, there has been little progress in statistical modeling of the geo-spatial nature of mental health events in Little Rock, Arkansas. In this article, we provide insights into the spatial nature of mental health data from Little Rock, Arkansas between 2015 and 2018, under a supervised spatial modeling framework. We provide evidence of spatial clustering and identify the important features influencing such heterogeneity via a spatially informed hierarchy of generalized linear, tree-based, and spatial regression models, viz. the Poisson regression model, the random forest model, the spatial Durbin error model, and the Manski model. The insights obtained from these different models are presented here along with their relative predictive performances. The inferential tools developed here can be used in a broad variety of spatial modeling contexts and have the potential to aid both law enforcement agencies and the city in properly allocating resources. We were able to identify several built-environment and socio-demographic measures related to mental health calls while noting that the results indicated that there are unmeasured factors that contribute to the number of events.
Optimized De Novo Eriodictyol Biosynthesis in Streptomyces albidoflavus Using an Expansion of the Golden Standard Toolkit for Its Use in Actinomycetes
Authors: Magadán-Corpas Patricia , Ye Suhui , Pérez-Valero Álvaro , McAlpine Patrick L. , Valdés-Chiara Paula , Torres-Bacete Jesús , Nogales Juan , Villar Claudio J. , Lombó Felipe
Published: May 2023

Eriodictyol is a hydroxylated flavonoid displaying multiple pharmaceutical activities, such as antitumoral, antiviral or neuroprotective. However, its industrial production is limited to extraction from plants due to its inherent limitations. Here, we present the generation of a Streptomyces albidoflavus bacterial factory edited at the genome level for an optimized de novo heterologous production of eriodictyol. For this purpose, an expansion of the Golden Standard toolkit (a Type IIS assembly method based on the Standard European Vector Architecture (SEVA)) has been created, encompassing a collection of synthetic biology modular vectors (adapted for their use in actinomycetes). These vectors have been designed for the assembly of transcriptional units and gene circuits in a plug-and-play manner, as well as for genome editing using CRISPR-Cas9-mediated genetic engineering. These vectors have been used for the optimization of the eriodictyol heterologous production levels in S. albidoflavus by enhancing the flavonoid-3′-hydroxylase (F3’H) activity (by means of a chimera design) and by replacing three native biosynthetic gene clusters in the bacterial chromosome with the plant genes matBC (involved in extracellular malonate uptake and its intracellular activation into malonyl-CoA), therefore allowing more malonyl-CoA to be devoted to the heterologous production of plant flavonoids in this bacterial factory. These experiments have allowed an increase in production of 1.8 times in the edited strain (where the three native biosynthetic gene clusters have been deleted) in comparison with the wild-type strain and a 13 times increase in eriodictyol overproduction in comparison with the non-chimaera version of the F3′H enzyme.
Antibacterial Evaluation of Zirconia Coated with Plasma-Based Graphene Oxide with Photothermal Properties
Authors: Park Lydia , Kim Hee-Seon , Jang Woohyung , Ji Min-Kyung , Ryu Je-Hwang , Cho Hoonsung , Lim Hyun-Pil
Published: May 2023

The alternative antibacterial treatment photothermal therapy (PTT) significantly affects oral microbiota inactivation. In this work, graphene with photothermal properties was coated on a zirconia surface using atmospheric pressure plasma, and then the antibacterial properties against oral bacteria were evaluated. For the graphene oxide coating on the zirconia specimens, an atmospheric pressure plasma generator (PGS-300, Expantech, Suwon, Republic of Korea) was used, and an Ar/CH4 gas mixture was coated on a zirconia specimen at a power of 240 W and a rate of 10 L/min. In the physiological property test, the surface properties were evaluated by measuring the surface shape of the zirconia specimen coated with graphene oxide, as well as the chemical composition and contact angle of the surface. In the biological experiment, the degree of adhesion of Streptococcus mutans (S. mutans) and Porphyromonas gingivalis (P. gingivalis) was determined by crystal violet assay and live/dead staining. All statistical analyzes were performed using SPSS 21.0 (SPSS Inc., Chicago, IL, USA). The group in which the zirconia specimen coated with graphene oxide was irradiated with near-infrared rays demonstrated a significant reduction in the adhesion of S. mutans and P. gingivalis compared with the group not irradiated. The oral microbiota inactivation was reduced by the photothermal effect on the zirconia coated with graphene oxide, exhibiting photothermal properties.
Repurposing Amphotericin B and Its Liposomal Formulation for the Treatment of Human Mpox
Authors: Peruzzu Daniela , Fecchi Katia , Venturi Giulietta , Gagliardi Maria Cristina
Published: May 2023

Mpox (monkeypox) is a zoonotic viral disease caused by the mpox virus (MPXV). Recently in 2022, a multi-country Mpox outbreak has determined great concern as the disease rapidly spreads. The majority of cases are being noticed in European regions and are unrelated to endemic travel or known contact with infected individuals. In this outbreak, close sexual contact appears to be important for MPXV transmission, and an increasing prevalence in people with multiple sexual partners and in men who have sex with men has been observed. Although Vaccinia virus (VACV)-based vaccines have been shown to induce a cross-reactive and protective immune response against MPXV, limited data support their efficacy against the 2022 Mpox outbreak. Furthermore, there are no specific antiviral drugs for Mpox. Host-cell lipid rafts are small, highly dynamic plasma-membrane microdomains enriched in cholesterol, glycosphingolipids and phospholipids that have emerged as crucial surface-entry platforms for several viruses. We previously demonstrated that the antifungal drug Amphotericin B (AmphB) inhibits fungal, bacterial and viral infection of host cells through its capacity to sequester host-cell cholesterol and disrupt lipid raft architecture. In this context, we discuss the hypothesis that AmphB could inhibit MPXV infection of host cells through disruption of lipid rafts and eventually through redistribution of receptors/co-receptors mediating virus entry, thus representing an alternative or additional therapeutic tool for human Mpox.
Human Umbilical Cord Mesenchymal Stem Cells Protect against Renal Ischemia-Reperfusion Injury by Secreting Extracellular Vesicles Loaded with miR-148b-3p That Target Pyruvate Dehydrogenase Kinase 4 to Inhibit Endoplasmic Reticulum Stress at the Reperfusion Stages
Authors: Shi Wei , Zhou Xiang , Li Xinyuan , Peng Xiang , Chen Guo , Li Yang , Zhang Chunlin , Yu Haitao , Feng Zhenwei , Gou Xin , Fan Jing
Published: May 2023

Renal ischemia-reperfusion (I/R) injury is a leading cause of acute kidney injury (AKI), with high mortality. Recent studies have reported that human umbilical cord mesenchymal stem cells (HucMSCs) play an important role in repairing organ and tissue injuries because of their unique characteristics. However, the potential of HucMSC extracellular vesicles (HucMSC-EVs) to promote the repair of renal tubular cells remains to be explored. This study found that HucMSC-EVs derived from HucMSCs played a protective role and were associated with kidney I/R injury. We found that miR-148b-3p in HucMSC-EVs had a protective effect against kidney I/R injury. HK-2 cells overexpressing miR-148b-3p were protected against I/R injury by inhibiting apoptosis. Next, the target mRNA of miR-148b-3p was predicted online, and the target mRNA, pyruvate dehydrogenase kinase 4 (PDK4), was identified and verified using dual luciferase. We discovered that I/R injury significantly increased endoplasmic reticulum (ER) stress, whereas siR-PDK4 inhibited these effects and protected against I/R injury. Interestingly, after administrating HucMSC-EVs to HK-2 cells, PDK4 expression and ER stress induced by I/R injury were significantly inhibited. HK-2 ingested miR-148b-3p from HucMSC-EVs, and its ER induced by I/R injury was significantly deregulated. This study suggests that HucMSC-EVs protect kidneys from I/R injury during the early I/R stage. These results suggest a new mechanism for HucMSC-EVs in treating AKI and provide a new treatment strategy for I/R injury.
Mitochondrial Features of Mouse Myoblasts Are Finely Tuned by Low Doses of Ozone: The Evidence In Vitro
Authors: Inguscio Chiara Rita , Dalla Pozza Elisa , Dando Ilaria , Boschi Federico , Tabaracci Gabriele , Angelini Osvaldo , Picotti Pietro Maria , Malatesta Manuela , Cisterna Barbara
Published: May 2023

The mild oxidative stress induced by low doses of gaseous ozone (O3) activates the antioxidant cell response through the nuclear factor erythroid 2-related factor 2 (Nrf2), thus inducing beneficial effects without cell damage. Mitochondria are sensitive to mild oxidative stress and represent a susceptible O3 target. In this in vitro study, we investigated the mitochondrial response to low O3 doses in the immortalized, non-tumoral muscle C2C12 cells; a multimodal approach including fluorescence microscopy, transmission electron microscopy and biochemistry was used. Results demonstrated that mitochondrial features are finely tuned by low O3 doses. The O3 concentration of 10 μg maintained normal levels of mitochondria-associated Nrf2, promoted the mitochondrial increase of size and cristae extension, reduced cellular reactive oxygen species (ROS) and prevented cell death. Conversely, in 20 μg O3-treated cells, where the association of Nrf2 with the mitochondria drastically dropped, mitochondria underwent more significant swelling, and ROS and cell death increased. This study, therefore, adds original evidence for the involvement of Nrf2 in the dose-dependent response to low O3 concentrations not only as an Antioxidant Response Elements (ARE) gene activator but also as a regulatory/protective factor of mitochondrial function.
Hamster Sperm Possess Functional Na+/Ca2+-Exchanger 1: Its Implication in Hyperactivation
Authors: Takei Gen L. , Ogura Yuhei , Ujihara Yoshihiro , Toyama Fubito , Hayashi Keitaro , Fujita Tomoe
Published: May 2023

Previous studies demonstrated that hamster sperm hyperactivation is suppressed by extracellular Na+ by lowering intracellular Ca2+ levels, and Na+/Ca2+-exchanger (NCX) specific inhibitors canceled the suppressive effects of extracellular Na+. These results suggest the involvement of NCX in the regulation of hyperactivation. However, direct evidence of the presence and functionality of NCX in hamster spermatozoa is still lacking. This study aimed to reveal that NCX is present and is functional in hamster spermatozoa. First, NCX1 and NCX2 transcripts were detected via RNA-seq analyses of hamster testis mRNAs, but only the NCX1 protein was detected. Next, NCX activity was determined by measuring the Na+-dependent Ca2+ influx using the Ca2+ indicator Fura-2. The Na+-dependent Ca2+ influx was detected in hamster spermatozoa, notably in the tail region. The Na+-dependent Ca2+ influx was inhibited by the NCX inhibitor SEA0400 at NCX1-specific concentrations. NCX1 activity was reduced after 3 h of incubation in capacitating conditions. These results, together with authors’ previous study, showed that hamster spermatozoa possesses functional NCX1 and that its activity was downregulated upon capacitation to trigger hyperactivation. This is the first study to successfully reveal the presence of NCX1 and its physiological function as a hyperactivation brake.
miR-100-5p Regulates Skeletal Muscle Myogenesis through the Trib2/mTOR/S6K Signaling Pathway
Authors: Wang Kaiming , Liufu Sui , Yu Zonggang , Xu Xueli , Ai Nini , Li Xintong , Liu Xiaolin , Chen Bohe , Zhang Yuebo , Ma Haiming , Yin Yulong
Published: May 2023

MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial regulatory roles in many biological processes, including the growth and development of skeletal muscle. miRNA-100-5p is often associated with tumor cell proliferation and migration. This study aimed to uncover the regulatory mechanism of miRNA-100-5p in myogenesis. In our study, we found that the miRNA-100-5p expression level was significantly higher in muscle tissue than in other tissues in pigs. Functionally, this study shows that miR-100-5p overexpression significantly promotes the proliferation and inhibits the differentiation of C2C12 myoblasts, whereas miR-100-5p inhibition results in the opposite effects. Bioinformatic analysis predicted that Trib2 has potential binding sites for miR-100-5p at the 3′UTR region. A dual-luciferase assay, qRT-qPCR, and Western blot confirmed that Trib2 is a target gene of miR-100-5p. We further explored the function of Trib2 in myogenesis and found that Trib2 knockdown markedly facilitated proliferation but suppressed the differentiation of C2C12 myoblasts, which is contrary to the effects of miR-100-5p. In addition, co-transfection experiments demonstrated that Trib2 knockdown could attenuate the effects of miR-100-5p inhibition on C2C12 myoblasts differentiation. In terms of the molecular mechanism, miR-100-5p suppressed C2C12 myoblasts differentiation by inactivating the mTOR/S6K signaling pathway. Taken together, our study results indicate that miR-100-5p regulates skeletal muscle myogenesis through the Trib2/mTOR/S6K signaling pathway.
Comprehensive Analysis of the NF-YB Gene Family and Expression under Abiotic Stress and Hormone Treatment in Larix kaempferi
Authors: Li Lu , Ren Xi , Shao Liying , Huang Xun , Zhang Chunyan , Wang Xuhui , Yang Jingli , Li Chenghao
Published: May 2023

NF-YB, a subfamily of Nuclear Factor Y (NF-Y) transcription factor, play crucial role in many biological processes of plant growth and development and abiotic stress responses, and they can therefore be good candidate factors for breeding stress-resistant plants. However, the NF-YB proteins have not yet been explored in Larix kaempferi, a tree species with high economic and ecological values in northeast China and other regions, limiting the breeding of anti-stress L. kaempferi. In order to explore the roles of NF-YB transcription factors in L. kaempferi, we identified 20 LkNF-YB family genes from L. kaempferi full-length transcriptome data and carried out preliminary characterization of them through series of analyses on their phylogenetic relationships, conserved motif structure, subcellular localization prediction, GO annotation, promoter cis-acting elements as well as expression profiles under treatment of phytohormones (ABA, SA, MeJA) and abiotic stresses (salt and drought). The LkNF-YB genes were classified into three clades through phylogenetic analysis and belong to non-LEC1 type NF-YB transcription factors. They have 10 conserved motifs; all genes contain a common motif, and their promoters have various phytohormones and abiotic stress related cis-acting elements. Quantitative real time reverse transcription PCR (RT-qPCR) analysis showed that the sensitivity of the LkNF-YB genes to drought and salt stresses was higher in leaves than roots. The sensitivity of LKNF-YB genes to ABA, MeJA, SA stresses was much lower than that to abiotic stress. Among the LkNF-YBs, LkNF-YB3 showed the strongest responses to drought and ABA treatments. Further protein interaction prediction analysis for LkNF-YB3 revealed that LkNF-YB3 interacts with various factors associated with stress responses and epigenetic regulation as well as NF-YA/NF-YC factors. Taken together, these results unveiled novel L. kaempferi NF-YB family genes and their characteristics, providing the basic knowledge for further in-depth studies on their roles in abiotic stress responses of L. kaempferi.
Gonadotropins and Sex Steroid Hormones in Captive-Reared Small Yellow Croaker (Larimichthys polyactis) and Their Role in Female Reproductive Dysfunction
Authors: Kho Kang Hee , Sukhan Zahid Parvez , Yang Seok-Woo , Hwang Nam-Yong , Lee Won-Kyo
Published: May 2023

The seed production of small yellow croaker (SYC) is constrained by reproductive dysfunction in captive-reared females. Reproductive dysfunction is closely linked to endocrine reproductive mechanisms. To better understand the reproductive dysfunction in captive broodstock, functional characterization of gonadotropins (GtHs: follicle stimulating hormone β subunit, fshβ; luteinizing hormone β subunit, lhβ; and glycoprotein α subunit, gpα) and sex steroids (17β-estradiol, E2; testosterone, T; progesterone; P) was performed using qRT-PCR, ELISA, in vivo, and in-vitro assay. The pituitary GtHs and gonadal steroids levels were significantly higher in ripen fish of both sexes. However, changes in lhβ and E2 levels in females were not significant in the developing and ripen stages. Furthermore, GtHs and steroids levels were lower in females compared to males throughout the reproductive cycle. In vivo administration of gonadotropin releasing hormone analogue (GnRHa) significantly increased the expression of GtHs in both dose- and time-related manners. The lower and higher doses of GnRHa led to successful spawning in male and female SYC, respectively. Sex steroids in vitro significantly inhibited the expression of lhβ in female SYC. Overall, GtHs were shown to play a vital role in final gonadal maturation, while steroids promoted negative feedback in the regulation of pituitary GtHs. Lower levels of GtHs and steroids might be key components in the reproductive dysfunction of captive-reared female SYC.
Performance and Mechanism of Functionalized Water Hyacinth Biochar for Adsorption and Removal of Benzotriazole and Lead in Water
Authors: Bian Pengyang , Shao Qinqin
Published: May 2023

In this paper, water hyacinth is used to prepare biochar (WBC). A biochar–aluminum–zinc-layered double hydroxide composite functional material (WL) is synthesized via a simple co-precipitation method which is used to adsorb and remove benzotriazole (BTA) and lead (Pb2+) in an aqueous solution. In particular, this research paper uses various characterization methods to analyze WL and to explore the adsorption performance and adsorption mechanism of WL on BTA and Pb2+ in an aqueous solution through batch adsorption experiments combined with model fitting and spectroscopy techniques. The results indicate that the surface of WL contains a thick sheet-like structure with many wrinkles which would provide many adsorption sites for pollutants. At room temperature (25 °C), the maximum adsorption capacities of WL on BTA and Pb2+ are 248.44 mg·g−1 and 227.13 mg·g−1, respectively. In a binary system, during the process of using WL to adsorb BTA and Pb2+, compared with that in the absorption on Pb2+, WL shows a stronger affinity in the adsorption on BTA, and BTA would thus be preferred in the absorption process. The adsorption process of WL on BTA and Pb2+ is spontaneous and is endothermic monolayer chemisorption. In addition, the adsorption of WL on BTA and Pb2+ involves many mechanisms, but the main adsorption mechanisms are different. Among them, hydrogen bonding dominates the adsorption on BTA, while functional groups (C-O and C=O) complexation dominates the adsorption on Pb2+. When WL adsorbs BTA and Pb2+, the coexistence of cations (K+, Na+, and Ca2+) has a strong anti-interference ability, and WL can use a lower concentration of fulvic acid (FA) (<20 mg·L−1) to improve its adsorption performance. Last but not least, WL has a stable regenerative performance in a one-component system and a binary system, which indicates that WL has excellent potential for the remediation of BTA and Pb2+ in water.
Rational Design of Bifunctional Microporous Organic Polymers Containing Anthracene and Triphenylamine Units for Energy Storage and Biological Applications
Authors: Mousa Aya Osama , Lin Zheng-Ian , Chuang Cheng-Hsin , Chen Chih-Kuang , Kuo Shiao-Wei , Mohamed Mohamed Gamal
Published: May 2023

In this study, we synthesized two conjugated microporous polymers (CMPs), An-Ph-TPA and An-Ph-Py CMPs, using the Suzuki cross-coupling reaction. These CMPs are organic polymers with p-conjugated skeletons and persistent micro-porosity and contain anthracene (An) moieties linked to triphenylamine (TPA) and pyrene (Py) units. We characterized the chemical structures, porosities, thermal stabilities, and morphologies of the newly synthesized An-CMPs using spectroscopic, microscopic, and N2 adsorption/desorption isotherm techniques. Our results from thermogravimetric analysis (TGA) showed that the An-Ph-TPA CMP displayed better thermal stability with Td10 = 467 °C and char yield of 57 wt% compared to the An-Ph-Py CMP with Td10 = 355 °C and char yield of 54 wt%. Furthermore, we evaluated the electrochemical performance of the An-linked CMPs and found that the An-Ph-TPA CMP had a higher capacitance of 116 F g−1 and better capacitance stability of 97% over 5000 cycles at 10 A g−1. In addition, we assessed the biocompatibility and cytotoxicity of An-linked CMPs using the MTT assay and a live/dead cell viability assay and observed that they were non-toxic and biocompatible with high cell viability values after 24 or 48 h of incubation. These findings suggest that the An-based CMPs synthesized in this study have potential applications in electrochemical testing and the biological field.
Using Construction and Demolition Waste Materials to Develop Chip Seals for Pavements
Authors: Shamsaei Mohsen , Carter Alan , Vaillancourt Michel
Published: May 2023

Construction and demolition waste (CDW) materials account for a considerable part of waste materials throughout the world. As these materials are not usually recycled, reusing them in construction projects is of major significance. In this study, recycled concrete, bricks, and glass were used as 100% aggregates of chip seal, which is a corrective or preventive pavement maintenance method. A cationic rapid setting (CRS-2) bitumen emulsion was also used to prepare the chip seal. Different tests, including the sand patch test, sweep test, British pendulum tester (BPT), interface bond, and Vialit test, were conducted. The results of these tests revealed that all these materials had sufficient aggregate embedment for vehicle speeds of more than 70 km/h, and the number of chips was less than 10%, indicating their good performance. All developed chip seals ranked as high skid resistance pavement at ambient temperature. The chip seals developed with concrete and glass showed the best adhesion with an asphalt pavement surface and an aggregate–bitumen adhesion at very cold and ambient temperatures due to the fact of their chemical compositions. Overall, using concrete aggregates to develop chip seals under different traffic loads is recommended. Finally, these findings can provide a novel approach for recycling CDW materials with low costs.
Free Shrinkage Strains of Box Girders with Concrete Overlays
Authors: Parmiani Maria Giulia , Orta Luis
Published: May 2023

The aging of reinforced concrete structures is one of the biggest concerns in civil engineering today since billions of dollars are spent annually on deck repairs and replacements. This study focuses on the rehabilitation of reinforced concrete box girders used in bridge construction. Bridge rehabilitation with a new concrete overlay possesses many challenges that involve cracking and debonding of the overlay caused by the restraining effect of the substrate. This effect leads to the development of tension stresses in the overlay, compression stresses in the substrate, and shear stresses at the interface. In order to mitigate this type of cracking and to ensure a desirable monolithic structural behavior of the rehabilitated bridge, a long-term assessment of the free shrinkage strains acting in the overlay is necessary. This study conducts a two-dimension finite element analysis of a reinforced concrete box girder bridge to evaluate humidity and free shrinkage strain profiles at different times. The humidity gradient between the overlay and the substrate generates differential volume changes between substrate and overlay. The substrate deformations are negligible, while the overlay is subjected to high shrinkage; 78% of the ultimate shrinkage strain is reached after 3 years, indicating a high susceptibility to cracking.
Positive Association of Serum Galectin-3 with the Development of Aortic Stiffness of Patients on Peritoneal Dialysis
Authors: Huang Po-Yu , Huang Chen-Sen , Lin Yu-Li , Chen Yi-Hsin , Hung Szu-Chun , Tsai Jen-Pi , Hsu Bang-Gee
Published: May 2023

A novel cardiovascular stress biomarker known as galectin-3 might be useful for anticipating adverse cardiovascular outcomes. The objective of the current investigation was to assess the association between serum galectin-3 levels and aortic stiffness (AS) in 196 patients on peritoneal dialysis. An enzyme-linked immunosorbent examination and a cuff-based volumetric displacement were employed to determine the levels of serum galectin-3 and the carotid-femoral pulse wave velocity (cfPWV), respectively. The AS group had 48 patients in total (24.5%) with cfPWV greater than 10 m/s. The AS group, when compared with the group without AS, had a significantly higher prevalence of diabetes mellitus and hypertension in addition to greater fasting glucose levels, waist circumference, systolic blood pressure, and serum galectin-3 levels. Multivariate logistic and linear regression analysis demonstrated that serum glactin-3 levels, in addition to gender and age, were significantly and independently associated with cfPWV and AS. Serum galectin-3 levels were linked with AS, according to a receiver operating characteristic curve analysis, with an area under the curve of 0.648 (95% confidence interval, 0.576–0.714; p = 0.0018). In summary, there was a significant correlation between serum galectin-3 levels and cfPWV in patients undergoing peritoneal dialysis therapy for end-stage kidney disease.
General Factors and Dental-Related Risk Factors for Postoperative Pneumonia or Infectious Complications: A Retrospective Study
Authors: Isomura Emiko Tanaka , Fujimoto Yukari , Matsukawa Makoto , Yokota Yusuke , Urakawa Ryuta , Tanaka Susumu
Published: May 2023

Numerous studies report that perioperative oral care decreases the frequency of postoperative pneumonia or infection. However, no studies have analyzed the specific impact of oral infection sources on the postoperative course, and the criteria for preoperative dental care differ among institutions. This study aimed to analyze the factors and dental conditions present in patients with postoperative pneumonia and infection. Our results suggest that general factors related to postoperative pneumonia, including thoracic surgery, sex (male > female), the presence or absence of perioperative oral management, smoking history, and operation time, were identified, but there were no dental-related risk factors associated with it. However, the only general factor related to postoperative infectious complications was operation time, and the only dental-related risk factor was periodontal pocket (4 mm or higher). These results suggest that oral management immediately before surgery is sufficient to prevent postoperative pneumonia, but that moderate periodontal disease must be eliminated to prevent postoperative infectious complication, which requires periodontal treatment not only immediately before surgery, but also on a daily basis.
Patient Selection Criteria and Procedural Standardization for Carotid Artery Stenting—A Single Center Experience
Authors: Calo Paolo , Oberhuber Alexander , Görtz Hartmut
Published: May 2023

The gold standard for the treatment of carotid artery stenosis is the carotid endarterectomy (CEA). According to current guidelines, carotid artery stenting (CAS) is an alternative. Randomized control trials (RCTs) show significantly higher rates of peri-interventional strokes after CAS compared to CEA. However, these trials were usually characterized by a great heterogeneity in the CAS procedure. In this retrospective analysis from 2012 to 2020, 202 symptomatic and asymptomatic patients were treated with CAS. Patients were carefully pre-selected according to anatomical and clinical criteria. In all cases, the same steps and material were used. All interventions were performed by five experienced vascular surgeons. Primary endpoints of this study were perioperative death and stroke. Asymptomatic carotid stenosis was present in 77% of the patients and symptomatic in 23%. The mean age was 66 years. The average degree of stenosis was 81%. The CAS technical success rate was 100%. Periprocedural complications occurred in 1.5% of cases, including one major stroke (0.5%) and two minor strokes (1%). The results of this study indicate that through a strict patient selection based on anatomical and clinical criteria, CAS can be performed with very low complication rates. Furthermore, standardization of the materials and the procedure itself is crucial.
Porous Diatomaceous Earth/Nano-Zinc Oxide Composites: Preparation and Antimicrobial Applications
Authors: Chung Chin-Chun , Hwang Jiunn-Jer
Published: May 2023

This paper presents the preparation and characterization of a porous and antimicrobial composite material consisting of diatomaceous earth, an inorganic pore-forming agent, and nano-zinc oxide (ZnO). A modified direct precipitation device produced high-surface area ZnO powder. The effect of reaction temperature, volume flow rate, and titration rate on ZnO particle size was studied. Using sodium chloride, potassium nitrate, and sodium percarbonate as porosity to create porous structures through dissolution was also investigated. This study found that adding cement sand to diatomaceous earth improved mold strength while lowering the volume flow rate, and increasing the reaction temperature increased the specific surface area of ZnO. At 60 °C, the crystalline structure changed from an irregular spherical form to a regular nanorod structure. The specific surface area of the prepared ZnO nanorods reached over 15 m2/g, which is about five times higher. In an antibacterial experiment, adding 5% ZnO nanorods of 50 nm diameter to the porous diatomaceous earth composite material resulted in a nearly 100% antibacterial rate against E. coli in an aqueous environment. The results suggest that the porous diatomaceous earth/nano-ZnO composite has potential applications as an antimicrobial material, and the modified direct precipitation method could have broader implications in materials science.