bluuebunny/crossref_metadata_2025 huggingface dataset:
Pirfenidone Prevents Heart Fibrosis during Chronic Chagas Disease Cardiomyopathy
Authors: Silva Tatiana Araújo , Thomas Diane , Siqueira-Neto Jair L. , Calvet Claudia Magalhaes
Published: July 2024

Cardiac fibrosis is a severe outcome of Chagas disease (CD), caused by the protozoan Trypanosoma cruzi. Clinical evidence revealed a correlation between fibrosis levels with impaired cardiac performance in CD patients. Therefore, we sought to analyze the effect of inhibitors of TGF-β (pirfenidone), p38-MAPK (losmapimod) and c-Jun (SP600125) on the modulation of collagen deposition in cardiac fibroblasts (CF) and in vivo models of T. cruzi chronic infection. Sirius Red/Fast Green dye was used to quantify both collagen expression and total protein amount, assessing cytotoxicity. The compounds were also used to treat C57/Bl6 mice chronically infected with T. cruzi, Brazil strain. We identified an anti-fibrotic effect in vitro for pirfenidone (TGF-β inhibitor, IC50 114.3 μM), losmapimod (p38 inhibitor, IC50 17.6 μM) and SP600125 (c-Jun inhibitor, IC50 3.9 μM). This effect was independent of CF proliferation since these compounds do not affect T. cruzi-induced host cell multiplication as measured by BrdU incorporation. Assays of chronic infection of mice with T. cruzi have shown a reduction in heart collagen by pirfenidone. These results propose a novel approach to fibrosis therapy in CD, with the prospect of repurposing pirfenidone to prevent the onset of ECM accumulation in the hearts of the patients.
Serum Vascular Adhesion Protein-1 and Endothelial Dysfunction in Hepatic Cirrhosis: Searching for New Prognostic Markers
Authors: Fasolato Silvano , Bonaiuto Emanuela , Rossetto Monica , Vanzani Paola , Ceccato Fabio , Vittadello Fabio , Zennaro Lucio , Rigo Adelio , Mammano Enzo , Angeli Paolo , Pontisso Patrizia , Di Paolo Maria Luisa
Published: July 2024

Endothelial dysfunction plays a key role in the development of liver cirrhosis. Among the biomarkers of endothelial dysfunction, the soluble form of Vascular Adhesion Protein-1 (sVAP-1) is an unconventional and less known adhesion molecule endowed also with amine oxidase activity. The aim of this study was to explore and correlate the behavior of sVAP-1 with that of the soluble vascular cell adhesion molecule-1 (sVCAM-1) and intercellular adhesion molecule-1 (sICAM-1) and with the severity of liver cirrhosis. A cross-sectional study was carried out by enrolling 28 controls, 59 cirrhotic patients without hepatocellular carcinoma, and 56 patients with hepatocellular carcinoma (HCC), mainly caused by alcohol abuse. The levels of adhesion molecules and of the pro-inflammatory cytokines (IL-6 and TNF-αα) were determined by immunoassay and the enzymatic activity of sVAP-1 by a fluorometric assay. In non-diabetic patients without HCC, a specific behavior of sVAP-1 was highlighted. Differently from sVCAM-1, sICAM-1, and cytokines, the sVAP-1 level was significantly increased only in the early stage of disease, and then, it decreased in the last stage (866 ± 390 ng/mL vs. 545 ± 316 ng/mL, in Child–Pugh class A vs. C, respectively, p < 0.05). Bivariate analysis correlates sVAP-1 to sVCAM-1, in the absence of HCC (Spearman’s rho = 0.403, p < 0.01). Multiple linear regression analysis revealed that sVCAM-1 appears to be a predictor of sVAP-1 (β coefficient = 0.374, p = 0.021). In conclusion, in non-diabetic and non-HCC cirrhotic patients, sVAP-1 may be a potential prognostic biomarker that, together with sVCAM-1 and pro-inflammatory cytokines, may provide information on the progression of sinusoidal liver endothelium damage.
The Diagnostic Role of Skin Manifestations in Rheumatic Diseases in Children: A Critical Review of Paediatric Vasculitis
Authors: Di Ludovico Armando , Rinaldi Marta , Lauriola Federico , Ciarelli Francesca , La Bella Saverio , Gualdi Giulio , Chiarelli Francesco , Bailey Kathryn , Breda Luciana
Published: July 2024

Skin lesions are frequently observed in children with rheumatic diseases, particularly in conditions such as IgA vasculitis (IgAV) and Kawasaki disease (KD). In paediatric vasculitis, the presence of skin lesions serves as an early indicator, emphasising the importance of timely diagnosis to prevent complications, such as cardiac or renal involvement. Conversely, autoinflammatory disorders like juvenile systemic lupus erythematosus (SLE) and juvenile dermatomyositis (DM) may manifest with cutaneous manifestations either at the onset of disease or during its progression. Identifying these skin lesions prior to the appearance of systemic symptoms offers an opportunity for early diagnosis and treatment, which has a positive influence on the outcomes. Additionally, it is noteworthy that specific rheumatological conditions, such as acute rheumatic fever (ARF) or oligoarticular or polyarticular forms of juvenile idiopathic arthritis (JIA), may exhibit occasional, but significant skin involvement, which is strongly correlated with an unfavourable prognosis. The assessment of skin is important in the holist approach to assessing patients for potentially systemic/multisystem disorder and helps distinguish discrete conditions.
Serum-Derived Macrophage-Activating Factor Exhibits Anti-Tumor Activity via M2-to-M1 Macrophage Reprogramming
Authors: Takara Tsuyoshi , Takara Rei , Kobayashi Aya , Shirakata Hina , Ambai Shinobu , Shinohara Yusei , Uto Yoshihiro
Published: July 2024

Many anti-tumor effects of group-specific component-derived macrophage-activating factors (GcMAFs) have been reported; however, the specific mechanisms remain unclear. Controlling tumor-associated macrophages (TAMs) in the tumor microenvironment is essential for cancer treatment. This study assessed the role of GcMAF in macrophage activation, elucidated the mechanisms by which it exerts its anti-tumor effects, and determined its effects on TAMs in the tumor microenvironment. GcMAF-stimulated RAW264.7 macrophages and EMT6 breast tumor cells were co-cultured in a 0.4 µm pore cell culture insert, and gene and protein expression and cell viability were evaluated. DNA microarray analysis of GcMAF-stimulated RAW264.7 cells was conducted. The induction of M2 RAW264.7 cells by interleukin (IL)-4 and IL-13 was analyzed. GcMAF stimulation increased the tumor necrosis factor-α and inducible nitric oxide synthase mRNA and protein levels in RAW264.7 cells but decreased the viability of co-cultured EMT6 cells. Although the details of the receptor or signal pathway of GcMAF are still unclear, these results were confirmed in the M2 RAW264.7 cells, suggesting that GcMAF exerts anti-tumor effects by inducing the differentiation of macrophages into the M1 type and reprogramming M2 macrophages to the M1 type. The anti-tumor activity of GcMAF via M2-to-M1 macrophage reprogramming could aid in developing novel cancer immunotherapies.
Importance of Host Feeding in the Biological Control of Insect Pests: Case Study of Egg Parasitoid Species (Hymenoptera: Chalcidoidea: Trichogrammatidae)
Authors: Cabello Tomas , Gallego Juan Ramón , Lopez Inmaculada , Gamez Manuel , Garay Jozsef
Published: July 2024

Over recent decades, intraguild predation (IGP) has attracted special attention, both from the theoretical and practical standpoints. The present paper addresses the interference competition between two Trichogramma species (egg parasitoids)—on the one hand, the extrinsic interactions (i.e., the indirect competition between female T. achaeae and T. brassicae), and on the other, the intrinsic interactions between the larvae of both species. Furthermore, T. achaeae is a better competitor than T. brassicae due to a dual mechanism—the former acts as a facultative hyperparasitoid of the latter, exclusively considering parasitism relationships as well as presenting predation activity by host feeding, which gives preference to eggs previously parasitized by T. brassicae over non-parasitized eggs. Both mechanisms are dependent on the prey density, which is demonstrated by a change in the functional response (i.e., the relationship between the numbers of prey attacked at different prey densities) of T. achaeae adult female—it changes from type II (i.e., initial phase in which the number of attacked targets increases hyperbolically and then reaches an asymptote, reflecting the handling capacity of the predator), in the absence of competition (an instantaneous search rate of a′ = 9.996 ± 4.973 days−1 and a handling time of Th = 0.018 ± 0.001 days), to type I (i.e., linear increase in parasitism rate as host densities rise, until reaching a maximum parasitism rate, and an instantaneous search rate of a′ = 0.879 ± 0.072 days−1 and a handling time of Th ≈ 0) when interference competition is present. These results show that there is a greater mortality potential of this species, T. achaeae, in conditions of competition with other species, T. brassicae in this case. Based on this, their implications in relation to the biological control of pests by parasitoid species are discussed.
Geometric Morphometrics and Genetic Diversity Analysis of Chalcidoidea (Diglyphus and Pachyneuron) at Various Elevations
Authors: Xi Ouyan , Zhang Shuli , Li Jinzhe , Hu Hongying , Bai Ming
Published: July 2024

Eulophidae and Pteromalidae are parasitic wasps with a global distribution and import for the biological control of pests. They can be distributed in different altitude regions, but their morphological and genetic adaptations to different altitudes are unclear. Here, we collected specimens that belong to Eulophidae and Pteromalidae from various altitudinal gradients, based on integrated taxonomic approaches to determine the species composition, and we analyzed their body shape and size from different altitudes using geometric morphometrics. Then, we performed an analysis of the D. isaea population’s haplotype genes to illustrate their genetic diversity. As a result, eight species that belong to two genera, Diglyphus Walker (Eulophidae) and Pachyneuron Walker (Pteromalidae), were identified, including two newly recorded species from China (D. chabrias and D. sabulosus). Through a geometric morphometrics analysis of body shape, we found that a narrow forewing shape and a widened thorax are the significant characteristics of adaptation to high-altitude environments in D. isaea and P. aphidis. Additionally, the body size studies showed a principal relationship between centroid size and altitude; the size of the forewings and thorax increases at higher altitudes. Next, using haplotype analysis, 32 haplotypes were found in seven geographic populations with high genetic diversity of this species. Our research provides preliminary evidence for the morphological and genetic diversity adaptation of parasitic wasps to extreme environments, and these data can provide important references for investigations on the ecological adaptability of parasitic wasps.
The Relevance of Family Language Policy in Germany and Italy in the Development of Child Bilingualism: The Role of Natural Translation
Authors: Licari Camilla , Perotto Monica
Published: July 2024

The purpose of this paper is to analyse the role of natural translation in heritage speakers’ bilingual communication in relation to the family language policies (FLP) adopted to maintain heritage language in Italian and German multilingual families. In order to investigate this, in spring 2023, a semi-structured questionnaire was administered to both parents and children. The sample consists of 60 Russian-speaking bilingual HS living in Italy and Germany, where they have access to regular primary education and attend, in some cases, private Russian courses or schools. The informants do not receive specific translation training from or into the Russian language (they only practice translation at school from or into Italian/German), and they translate, in most cases, as an occasional activity, closer to the function of mediation or brokering. The role of translation in relation to FLP seems particularly relevant when comparing the two samples, considering different family compositions: mostly bi-ethnic in Italy and mono-ethnic in Germany. The survey showed that in daily life, both parents and children use translation, often as a specific kind of bilingual communication. In the Italian part of the sample, the strategy called OPOL prevails, and translation is a frequent activity in the domestic sphere. In the German one, instead, the separation of language use contexts is widespread, and all family members speak both Russian and German, making translation activity less relevant.
Retention Rate of Ixekizumab in Psoriatic Arthritis: A Real-World Study
Authors: Bellis Elisa , Ruscitti Piero , Donzella Denise , Crepaldi Gloria , Data Valeria , Gammino Marinella , Gatto Mariele , Guardo Valeria , Lomater Claudia , Marucco Elena , Saracco Marta , Iagnocco Annamaria
Published: July 2024

We aimed to examine the drug retention rate (DRR) of the interleukin-17 inhibitor ixekizumab in a real-world monocentric cohort of psoriatic arthritis (PsA) patients and to assess the predictors of drug discontinuation. Consecutive PsA patients who underwent treatment with ixekizumab from October 2019 to February 2023 were enrolled in this observational, retrospective, monocentric study. Clinical records were assessed at baseline and throughout the follow-up period. We collected sociodemographic data, smoking habits, body mass index, the presence of Human Leukocyte Antigen B27, comorbidities, disease involvement and duration, previous therapy, discontinuation of ixekizumab, reasons for discontinuation, and adverse events (AEs). DRR was evaluated as time to drug discontinuation and assessed through Kaplan–Meier curves. Baseline factors predicting drug discontinuation were investigated through logistic regression models. Eighty PsA patients were included in this study. Ixekizumab was administered at a dose of 160 mg by subcutaneous injection at baseline, followed by 80 mg every four weeks thereafter. Ixekizumab had a 38-month-cumulative DRR of 43.8%, accounting for both inefficacy and AEs. When considering only inefficacy, the DRR was 62.6%. Comorbidities (p = 0.665), obesity (p = 0.665), smoking (p = 0.884), disease duration ≤ 2 years (p = 0.071), axial (p = 0.131) and skin involvement (p = 0.460), and previous therapies, including conventional synthetic (p = 0.504) and biological (p = 0.474) Disease-Modifying Antirheumatic Drugs (bDMARDs), as well as the number of previous bDMARDs or targeted synthetic Disease-Modifying Antirheumatic Drugs (tsDMARDs), did not significantly affect the DRR (p = 0.349). Multivariate analysis found no independent predictors of drug discontinuation. The most frequent AEs leading to discontinuation were skin reactions; no severe infections were observed. In our real-world study, comorbidities, disease duration, and previous therapies did not affect the DRR of ixekizumab. Ixekizumab had a favorable safety profile, with no severe AEs observed.
A VMD-BP Model to Predict Laser Welding Keyhole-Induced Pore Defect in Al Butt–Lap Joint
Authors: Wang Wei , Dong Yang , Liu Fuyun , Yang Biao , Han Xiaohui , Wei Lianfeng , Song Xiaoguo , Tan Caiwang
Published: July 2024

The detection of keyhole-induced pore positions is a critical procedure for assessing laser welding quality. Considering the detection error due to pore migration and noise interference, this research proposes a regional prediction model based on the time–frequency-domain features of the laser plume. The original plume signal was separated into several signal segments to construct the morphological sequences. To suppress the mode mixing caused by environmental noise, variational modal decomposition (VMD) was utilized to process the signals. The time–frequency features extracted from the decomposed signals were acquired as the input of a backpropagation (BP) neural network to predict the pore locations. To reduce the prediction error caused by pore migration, the effect of the length of the signal segments on the prediction accuracy was investigated. The results show that the optimal signal segment length was 0.4 mm, with an accuracy of 97.77%. The 0.2 mm signal segments failed to eliminate the negative effects of pore migration. The signal segments over 0.4 mm resulted in prediction errors of small and dense pores. This work provides more guidance for optimizing the feature extraction of welding signals to improve the accuracy of welding defect identification.
Experimental Study on the Effects of Carbonated Steel Slag Fine Aggregate on the Expansion Rate, Mechanical Properties and Carbonation Depth of Mortar
Authors: Gao Pengfei , Wang Jian , Cui Jianjun , Yuan Yongyu , Song Yuanyuan
Published: July 2024

Steel slag is the main by-product of the steel industry and can be used to produce steel slag fine aggregate (SSFA). SSFA can be used as a fine aggregate in mortar or concrete. However, SSFA contains f-CaO, which is the main reason for the expansion damage of mortar and concrete. In this study, the carbonation treatment of SSFA was adopted to reduce the f-CaO content; the influence of the carbonation time on the content of f-CaO in the SSFA was studied; and the effects of the carbonated SSFA replacement ratio on the expansion rate, mechanical properties and carbonation depth of mortar were investigated through tests. The results showed that as the carbonation time increased, the content of f-CaO in the SSFA gradually decreased. Compared to the mortar specimens with carbonated SSFA, the specimens with uncarbonated SSFA showed faster and more severe damage and a higher expansion rate. When the replacement ratio of carbonated SSFA was less than 45%, the carbonated SSFA had an inhibitory effect on the expansion development of the specimens. The compressive strengths of the specimens with a carbonated SSFA replacement ratio of 60% and 45% were 1.29% and 6.81% higher than those of the specimens with an uncarbonated SSFA replacement ratio of 60% and 45%, respectively. Carbonation treatment could improve the replacement ratio of SSFA while ensuring the compressive strength of specimens. Compared with mortar specimens with uncarbonated SSFA, the anti-carbonation performance of mortar specimens with carbonated SSFA was reduced.
Development and Implementation of Die Forging Technology Eliminating Flange Welding Operations in Conveyor Driver Forging
Authors: Hawryluk Marek , Polak Sławomir , Rychlik Marcin , Barełkowski Artur , Jakuć Jakub , Marzec Jan
Published: July 2024

This article presents research results regarding the development of a new manufacturing technology for an element assigned to belt conveyor flights in the extractive industry through hot die forging (of a forging with a double-sided flange) instead of the currently realized process of producing such an element by welding two flanges onto a sleeve or one flange onto a flange forging. The studies were conducted to design an innovative and low-waste technology, mainly with the use of numerical modelling and simulations, partially based on the current technology of producing a flange forging. Additionally, during the development of the forging process, the aspect of robotization was considered, both in respect of the forging tools and the process of transportation and relocation of forging between the impressions and the forging aggregates. A thermo-mechanical model of the process of producing a belt conveyor flight forging with deformable tools was elaborated by means of the Forge 3NxT program. The results of the conducted numerical modelling made it possible, among other things, to develop models of forging tools ensuring the proper manner of material flow and filling of the impressions, as well as temperature and plastic deformation distributions in the forging and also the detection of possible forging defects. For the technology elaborated this way, the tools were built together with a special instrument for flanging in the metal, and technological tests were performed under industrial conditions. The produced forgings were verified through a measurement of the geometry, by way of 3D scanning, as well as the hardness, which definitively confirmed the properness of the developed technology. The obtained technological test results made it possible to confirm that the elaborated construction, as well as the tool impressions, ensure the possibility of implementing the designed technology with the use of robotization and automatization of the forging process.
Proposal for New Method for Calculating Sedimentation Process Efficiency in Water Treatment Plants
Authors: Banaś Marian , Hilger Bartłomiej
Published: July 2024

An important aspect of water treatment is removing fine-grain materials from water. Due to the properties of fine-grain materials, they are difficult to remove from water. During the sedimentation process, which takes place in settling tanks, such materials are removed. The sedimentation process is often accompanied by coagulation and flocculation processes, which form aggregates of particles (flocs) from the fine-grained material particles in a suspension (non-grainy suspension). This kind of suspension (consisting of aggregates of particles or flocs) shows a different behaviour when falling compared with classic grainy suspensions. The main goal and novelty of this article are to propose (and test) a modification of the often used Stokes’ formula with the addition of fractal geometry into the calculation of the terminal velocity of free-falling particles in order to overcome Stokes’ formula’s limitation, thus obtaining the sedimentation process efficiency. Because of this fractal modification, it is possible to use the simple and elegant Stokes’ formula in order to calculate better the terminal velocity of non-grainy particles—aggregates or flocs—and thus obtain the sedimentation efficiency for the whole range of suspensions, both non-grainy and grainy. The results obtained in this article show that the sedimentation process efficiency calculated by using the modified formula based on the fractal geometry morphology of particles (the proposed fractal method) describes and agrees more with the data from the experiment than the sedimentation efficiency calculated only based on particle size (classic method).
A Pragmatic Approach for Rapid, Non-Destructive Assessment of Defect Types in Laser Powder Bed Fusion Based on Melt Pool Monitoring Data
Authors: Engelhardt Anna , Wegener Thomas , Niendorf Thomas
Published: July 2024

Process monitoring systems, e.g., systems based on photodiodes, could be used in laser-based powder bed fusion (PBF-LB/M) to measure various process parameters and process signatures to eventually allow for a local, detailed analysis of the produced parts. Here, simple statements only concerning the occurrence of defects in parts are sufficient in many cases, especially with respect to industrial application. Therefore, a pragmatic approach to rapidly infer the occurrence of defects and their types based on in situ data obtained by commercially available process monitoring systems is introduced. In this approach, a color distribution in form of a histogram is determined for each produced part using layer-wise screenshots of the visualized data provided by the monitoring software. Assessment of the histograms of AlSi10Mg samples, which were processed with different parameter combinations, revealed characteristics depending on the prevailing defect types. These characteristics enable the prediction of the occurring defect types without the necessity to apply conventional downstream testing methods, and thus, a straightforward separation of parts with good quality from defective components. Since the approach presented uses the data visualization of the monitoring software, it can be used even when direct access to the raw data is not provided by the machine manufacturer.
An Update to The Demagnetizing Factor Dataset Calculated for The General Ellipsoid by Osborn
Authors: Kiss László F. , Bakonyi Imre
Published: July 2024

The exact formulae for calculating the demagnetizing factors of a general ellipsoid along the three main axes a ≥ b ≥ c have been long known. According to these formulae, the demagnetizing factors depend only on the axial ratios b/a and c/a. Although the calculation of the demagnetizing factors is a straightforward task, the calculation itself is not a simple one. Therefore, tabular and graphical representations of these demagnetizing factor data have also been presented which can then be used for approximating the demagnetizing factors of a rectangular ferromagnetic slab with the same axial ratios. It turned out in our recent study, however, that, in some ranges of axial ratios (e.g., for very small c/a values), the available tables and graphs do not provide sufficient resolution for obtaining the demagnetizing factors with reasonable accuracy. It was decided to calculate these missing values, and they are presented here in both tabular and graphical form by giving instructions for how to obtain conveniently further interpolated data. In addition, the previous and current demagnetizing factor data have been replotted and fitted to a polynomial function with high accuracy. The functional form of these fitting polynomials is presented in a table for the whole range of the axial ratios b/a and c/a. By graphically displaying these functions, one can obtain, in a relatively simple manner, the demagnetizing factors of a general ellipsoid with known axial ratios without the need to directly calculate through the exact formulae. This may be helpful in obtaining a quick estimate for the demagnetizing factors of any rectangular ferromagnetic slab of interest.
Total Synthesis and Biological Profiling of Putative (±)-Marinoaziridine B and (±)-N-Methyl Marinoaziridine A
Authors: Buljan Anđela , Stepanić Višnja , Čikoš Ana , Babić Brčić Sanja , Bojanić Krunoslav , Roje Marin
Published: July 2024

The total synthesis of two new marine natural products, (±)-marinoaziridine B 7 and (±)-N-methyl marinoaziridine A 8, was accomplished. The (±)-marinoaziridine 7 was prepared in a six-step linear sequence with a 2% overall yield. The key steps in our strategy were the preparation of the chiral epoxide (±)-5 using the Johnson Corey Chaykovsky reaction, followed by the ring-opening reaction and the Staudinger reaction. The N,N-dimethylation of compound (±)-7 gives (±)-N-methyl marinoaziridine A 8. The NMR spectra of synthetized (±)-marinoaziridine B 7 and isolated natural product did not match. The compounds are biologically characterized using relevant in silico, in vitro and in vivo methods. In silico ADMET and bioactivity profiling predicted toxic and neuromodulatory effects. In vitro screening by MTT assay on three cell lines (MCF-7, H-460, HEK293T) showed that both compounds exhibited moderate to strong antiproliferative and cytotoxic effects. Antimicrobial tests on bacterial cultures of Escherichia coli and Staphylococcus aureus demonstrated the dose-dependent inhibition of the growth of both bacteria. In vivo toxicological tests were performed on zebrafish Danio rerio and showed a significant reduction of zebrafish mortality due to N-methylation in (±)-8.
Metabolomics of Benzene Exposure and Development of Biomarkers for Exposure Hazard Assessment
Authors: Li Hao , Sun Qianyu , Li Fei , Wang Boshen , Zhu Baoli
Published: July 2024

Benzene, a common industrial solvent, poses significant health risks including poisoning and hematopoietic diseases. However, its precise toxicity mechanisms remain unclear. To assess the health impact of prolonged benzene exposure through metabolomic analyses of exposed workers and benzene-poisoned mice, aiming to identify biomarkers and minimize occupational hazards. This study compared 18 benzene-exposed workers with 18 non-exposed workers, matching for age, lifestyle, and BMI. The metabolites in the workers’ samples were analyzed using ultra-high-performance liquid chromatography and mass spectrometry. A larger study included 118 exposed and 158 non-exposed workers, incorporating surveys and routine blood and urine tests with differential metabolites targeted via an enzyme-linked immunosorbent assay. The animal studies consisted of two 15- and 60-day benzene staining and control experiments on 28 C57BL/6J mice, followed by sample collection and organ analysis. The data analysis employed eXtensible Computational Mass Spectrometry (XCMS), Python, MetaboAnalyst 6.0, and SPSS24.0. The exposed workers exhibited altered metabolites indicating external benzene exposure, lower glucose levels, and changes in white blood cell counts and urinary ketone bodies. The plasma metabolomics revealed disturbances in energy and lipid metabolism. The benzene-exposed mice displayed reduced weight gain, behavioral changes, and organ damage. Oxidative stress and abnormal purine and lipid metabolism were observed in both the long-term benzene-exposed workers and benzene-exposed mice. Metabolic markers for the early detection of benzene exposure hazards were identified, underscoring the need to mitigate occupational risks.
Photoperiod Regulates Aerobic Methane Emissions by Altering Plant Growth and Physiological Processes
Authors: Qaderi Mirwais M. , Burton Kate
Published: July 2024

Previous studies have shown that light quality and quantity affect methane emissions from plants. However, the role of photoperiod in plant-derived methane has not been addressed. We studied the effects of two photoperiods—long-day (16 h light/8 h dark), and short-day (8 h light/16 h dark)—on growth and methane emissions of lettuce (a long-day plant), mung bean (a short-day plant), and tomato (a day-neutral plant) under a temperature regime of 22/18 °C. All species were grown under both light durations. First, seeds were germinated in Petri dishes for one week, then plants were transferred to pots and randomly assigned to one of the two experimental conditions. Under each condition, twelve plants were grown for 21 days; at that time, plant growth and physiological traits, including plant dry mass, growth index, photosynthesis, chlorophyll fluorescence, total chlorophyll, nitrogen balance index, flavonoids, and anthocyanin, were measured. Lettuce plants under the short-day photoperiod had the highest methane emissions. Long-day plants that were exposed to short-day conditions and short-day plants that were exposed to long-day conditions were stressed; day-neutral plants were also stressed under short days (p < 0.05). All three species had decreased total dry mass under short-day conditions, most likely because of decreased photosynthesis and increased transpiration and stomatal conductance. Methane emission was positively correlated with shoot/root mass ratio, nonphotochemical quenching and anthocyanin; but was negatively correlated with stem height, dry mass, photosynthesis, water-use efficiency, total chlorophyll, and flavonoids (p < 0.05). This study revealed that, besides light intensity and quality, light duration can also affect methane emissions from plants.
A Lego-Like Reconfigurable Microfluidic Stabilizer System with Tunable Fluidic RC Constants and Stabilization Ratios
Authors: Zhuge Wuyang , Li Weihao , Wang Kaimin , Chen Zhuodan , Wu Chunhui , Jiang Kyle , Ding Jun , Anthony Carl , Cheng Xing
Published: July 2024

In microfluidic systems, it is important to maintain flow stability to execute various functions, such as chemical reactions, cell transportation, and liquid injection. However, traditional flow sources, often bulky and prone to unpredictable fluctuations, limit the portability and broader application of these systems. Existing fluidic stabilizers, typically designed for specific flow sources, lack reconfigurability and adaptability in terms of the stabilization ratios. To address these limitations, a modular and standardized stabilizer system with tunable stabilization ratios is required. In this work, we present a Lego-like modular microfluidic stabilizer system, which is fabricated using 3D printing and offers multi-level stabilization combinations and customizable stabilization ratios through the control of fluidic RC constants, making it adaptable to various microfluidic systems. A simplified three-element circuit model is used to characterize the system by straightforwardly extracting the RC constant without intricate calculations of the fluidic resistance and capacitance. By utilizing a simplified three-element model, the stabilizer yields two well-fitted operational curves, demonstrating an R-square of 0.95, and provides an optimal stabilization ratio below 1%. To evaluate the system’s effectiveness, unstable input flow at different working frequencies is stabilized, and droplet generation experiments are conducted and discussed. The results show that the microfluidic stabilizer system significantly reduces flow fluctuations and enhances droplet uniformity. This system provides a new avenue for microfluidic stabilization with a tunable stabilization ratio, and its plug-and-play design can be effectively applied across diverse applications to finely tune fluid flow behaviors in microfluidic devices.
A Low-Power and Robust Micromachined Thermal Convective Accelerometer
Authors: Ye Yizhou , Wan Shu , Hou Chen , He Xuefeng , Li Shunbo
Published: July 2024

This paper presents a micromachined thermal convective accelerometer with low power and high reliability. This accelerometer comprises a heater and two thermistors. The central heater elevates the temperature of the chip above ambient levels while the symmetrically arranged thermistors monitor the temperature differentials induced by acceleration. The heater and thermistors are fabricated on a glass substrate using a standard micro-electromechanical systems (MEMS) process flow, and the fabricated sensor is installed on a rotation platform and a shaking table experimental setup to perform the experiment. The results indicate that the sensor has the capability to measure accelerations surpassing 80 m/s2, with an approximate linear sensitivity of 110.69 mV/g. This proposed thermal convective accelerometer offers promising potential for various applications requiring precise acceleration measurements in environments where low power consumption and high reliability are paramount.
A Review of the Vaporization Behavior of Some Metal Elements in the LPBF Process
Authors: Shi Guanglei , Zhang Runze , Cao Yachao , Yang Guang
Published: July 2024

Metal additive manufacturing technology has developed by leaps and bounds in recent years; selective laser melting technology is a major form in metal additive manufacturing, and its application scenarios are numerous. For example, it is involved in many fields including aerospace field, automotive, mechanical processing, and the nuclear industry. At the same time, it also indirectly provides more raw materials for all walks of life in our country. However, during the selective laser melting process, due to the action of high-energy-density lasers, the temperature of most metal powders can reach above the vaporization temperature. Light metals with relatively low vaporization temperatures such as magnesium and zinc have more significant vaporization and other behaviors. At the same time, during the metal vaporization process, a variety of by-products are generated, which seriously affect the forming quality and mechanical properties of the workpiece, resulting in the workpiece quality possibly not reaching the expected target. This paper mainly interprets the metal vaporization behavior in the LPBF process and summarizes the international research progress and suppression methods for vaporization.
Computational and Experimental Characterization of Aligned Collagen across Varied Crosslinking Degrees
Authors: Lin Shengmao , Patrawalla Nashaita Y. , Zhai Yingnan , Dong Pengfei , Kishore Vipuil , Gu Linxia
Published: July 2024

Collagen-based scaffolds have been widely used in tissue engineering. The alignment of collagen fibers and the degree of crosslinking in engineering tissue scaffolds significantly affect cell activity and scaffold stability. Changes in microarchitecture and crosslinking degree also impact the mechanical properties of collagen scaffolds. A clear understanding of the effects of collagen alignment and crosslinking degrees can help properly control these critical parameters for fabricating collagen scaffolds with desired mechanical properties. In this study, combined uniaxial mechanical testing and finite element method (FEM) were used to quantify the effects of fiber alignment and crosslinking degree on the mechanical properties of collagen threads. We have fabricated electrochemically aligned collagen (ELAC) and compared it with randomly distributed collagen at varying crosslinking degrees, which depend on genipin concentrations of 0.1% or 2% for crosslinking durations of 1, 4, and 24 h. Our results indicate that aligned collagen fibers and higher crosslinking degree contribute to a larger Young’s modulus. Specifically, aligned fiber structure, compared to random collagen, significantly increases Young’s modulus by 112.7% at a 25% crosslinking degree (0.1% (4 h), i.e., 0.1% genipin concentration with a crosslinking duration of 4 h). Moreover, the ELAC Young’s modulus increased by 90.3% as the crosslinking degree doubled by changing the genipin concentration from 0.1% to 2% with the same 4 h crosslinking duration. Furthermore, verified computational models can predict mechanical properties based on specific crosslinking degrees and fiber alignments, which facilitate the controlled fabrication of collagen threads. This combined experimental and computational approach provides a systematic understanding of the interplay among fiber alignment, crosslinking parameters, and mechanical performance of collagen scaffolds. This work will enable the precise fabrication of collagen threads for desired tissue engineering performance, potentially advancing tissue engineering applications.
Assessing the Effect of Twisting and Twisting Fatigue on ZnO:Al Thin Film Performance on PEN and PET Substrates
Authors: Mohammed Dilveen W. , Ameen Rayan M. , Waddingham Rob , Flewitt Andrew J. , Bowen James , Kukureka Stephen N.
Published: July 2024

This study examines the electromechanical characteristics of aluminium-doped zinc oxide (AZO) films. The films were produced using the RF magnetron sputtering process with a consistent thickness of 150 nm on various polymer substrates. The study focuses on assessing the electro-mechanical failure processes of coated segments using flexible substrates, namely polyethylene naphthalate (PEN) and polyethylene terephthalate (PET), with a specific emphasis on typical cracking and delamination occurrences. This examination involves conducting twisting deformation together with using standardised electrical resistance measurements and optical microscope monitoring instruments. It was found that the crack initiation angle is mostly dependent on the mechanical mismatch between the coating and substrate. Higher critical twisting angle values are observed for the AZO/PEN film during twisting testing. Relative to the perpendicular plane of the untwisted sample, it was found that cracks initiated at a twist angle equal to 42° ± 2.1° and 38° ± 1.7° for AZO/PEN and AZO/PET, respectively, and propagated along the sample length. SEM images indicate that the twisting motion results in deformation in the thin film material, leading to the presence of both types of stress in the film structure. These discoveries emphasise the significance of studying the mechanical properties of thin films under different stress conditions, as it can impact their performance and reliability in real-world applications. The electromechanical stability of AZO was found to be similar on both substrates during fatigue testing. Studying the electromechanical properties of various material combinations is important for selecting polymer substrates and predicting the durability of flexible electronic devices made from polyester.
Research on a Support-Free Five-Degree-of-Freedom Additive Manufacturing Method
Authors: Han Xingguo , Wu Gaofei , Liu Xuan , Song Xiaohui , Cui Lixiu
Published: July 2024

When using traditional 3D printing equipment to manufacture overhang models, it is often necessary to generate support structures to assist in the printing of parts. The post-processing operation of removing the support structures after printing is time-consuming and wastes material. In order to solve the above problems, a support-free five-degree-of-freedom additive manufacturing (SFAM) method is proposed. Through the homogeneous coordinate transformation matrix, the forward and inverse kinematics equations of the five-degree-of-freedom additive manufacturing device (FAMD) are established, and the joint variables of each axis are solved to realize the five-axis linkage of the additive manufacturing (AM) device. In this research work, initially, the layered curve is obtained through the structural lines of the overhang model, and a continuous path planning of the infill area is performed on it, and further, the part printing experiments are conducted on the FAMD. Compared with the traditional three-axis additive manufacturing (TTAM) method, the SFAM method shortens the printing time by 23.58% and saves printing materials by 33.06%. The experimental results show that the SFAM method realizes the support-free printing of overhang models, which not only improves the accuracy of the parts but also the manufacturing efficiency of the parts.
Development of Light-Scribing Process Using L-Ascorbic Acid for Graphene Micro-Supercapacitor
Authors: Park Seorin , Lee Da Young , Cho Sunghun
Published: July 2024

The rapid development of smart technologies is accelerating the growing demand for microscale energy storage devices. This work reports a facile and practical approach to fabricating interdigitated graphene micro-patterns through the LSC process accompanied by the l-ascorbic acid (L-AA) and preheating treatment. Our work offered a higher degree of GO reduction than the conventional microfabrication. It significantly shortened the overall processing time to obtain the micro-patterns with improved electrical and electrochemical performances. The interdigitated MSC composed of 16 electrodes exhibited a high capacitance of 14.1 F/cm3, energy density of 1.78 mWh/cm3, and power density of 69.9 mW/cm3. Furthermore, the fabricated MSC device demonstrated excellent cycling stability of 88.2% after 10,000 GCD cycles and a high rate capability of 81.1% at a current density of 1.00 A/cm3. The fabrication process provides an effective means for producing high-performance MSCs for miniaturized electronic devices.
Real-Time Plasmonic Strain Sensors Based on Surface Relief Diffraction Gratings
Authors: Bdour Yazan , Sabat Ribal Georges
Published: July 2024

Large-scale diffraction gratings were fabricated in surface relief on azobenzene thin films and transferred to flexible PDMS substrates using soft lift-off lithography. The PDMS gratings were strained along the grating vector axis and the resulting surface topography was analyzed using diffraction angle measurements, AFM imagery and surface plasmon resonance (SPR) spectra. All measurement methods exhibited a linear response in strain indicating the useability of these sensors in real-world applications. For SPR-based strain sensing, an increasing pitch and a decreasing modulation depth were observed with increasing strain. The SPR peak shifted by ~1.0 nm wavelength and the SPR intensity decreased by ~0.3 a.u. per percentage of applied strain. The tested PDMS samples retained their integrity even after multiple cycles of stretching and relaxation, making them a suitable strain sensor.
Towards High-Performance Pockels Effect-Based Modulators: Review and Projections
Authors: Li Yu , Sun Muhan , Miao Ting , Chen Jianping
Published: July 2024

The ever-increasing demand for high-speed data transmission in telecommunications and data centers has driven the development of advanced on-chip integrated electro-optic modulators. Silicon modulators, constrained by the relatively weak carrier dispersion effect, face challenges in meeting the stringent requirements of next-generation photonic integrated circuits. Consequently, there has been a growing interest in Pockels effect-based electro-optic modulators, leveraging ferroelectric materials like LiNbO3, BaTiO3, PZT, and LaTiO3. Attributed to the large first-order electro-optic coefficient, researchers have delved into developing modulators with expansive bandwidth, low power consumption, compact size, and linear response. This paper reviews the working principles, fabrication techniques, integration schemes, and recent highlights in Pockels effect-based modulators.
Optimization of Grayscale Lithography for the Fabrication of Flat Diffractive Infrared Lenses on Silicon Wafers
Authors: Bouchouri Angelos , Akram Muhammad Nadeem , Øhlckers Per Alfred , Chen Xuyuan
Published: July 2024

Grayscale lithography (GSL) is an alternative approach to the standard binary lithography in MEMS fabrication, enabling the fabrication of complicated, arbitrary 3D structures on a wafer without the need for multiple masks and exposure steps. Despite its advantages, GSL’s effectiveness is highly dependent on controlled lab conditions, equipment consistency, and finely tuned photoresist (PR) exposure and etching processes. This works presents a thorough investigation of the challenges of GSL for silicon (Si) wafers and presents a detailed approach on how to minimize fabrication inaccuracies, aiming to replicate the intended design as closely as possible. Utilizing a maskless laser writer, all aspects of the GSL are analyzed, from photoresist exposure parameters to Si etching conditions. A practical application of GSL is demonstrated in the fabrication of 4-μm-deep f#/1 Si Fresnel lenses for long-wave infrared (LWIR) imaging (8–12 μm). The surface topography of a Fresnel lens is a good case to apply GSL, as it has varying shapes and size features that need to be preserved. The final fabricated lens profiles show a good match with the initial design, and demonstrate successful etching of coarse and fine features, and demonstrative images taken with an LWIR camera.
Independent Concentration Manipulation Using Sidewall-Driven Micromixer
Authors: Takayama Toshio , Maki Hayato
Published: July 2024

Lab-on-a-chip technology has been developed to streamline biochemical experiments by providing experimental environments in microscopic areas. Due to the difficulty of mixing chemicals in such small channels, various micromixers have been created. Our proposed sidewall-driven micromixer offers easy fabrication and precise control over mixing concentrations. In our previous study, we successfully generated concentration gradients by simultaneously mixing multiple chambers using a single actuator. However, it is not possible to mix different chemicals in each chamber. In this study, we developed a sidewall-driven micromixer that enables independent mixing in each chamber by installing one actuator per chamber. Experimental results showed that different conditions were achieved in each chamber using both microbead-mixture water and colored water. Thus, this mixer can be used to manipulate concentrations regardless of whether the mixing targets are particles or fluids.
Experimental Proof of Principle of 3D-Printed Microfluidic Benthic Microbial Fuel Cells (MBMFCs) with Inbuilt Biocompatible Carbon-Fiber Electrodes
Authors: Hornik Terak , Terry Maxwell , Krause Michael , Catterlin Jeffrey K. , Joiner Kevin L. , Aragon Samuel , Sarmiento Angelica , Arias-Thode Yolanda Meriah , Kartalov Emil P.
Published: July 2024

Microbial fuel cells (MFCs) represent a promising avenue for sustainable energy production by harnessing the metabolic activity of microorganisms. In this study, a novel design of MFC—a Microfluidic Benthic Microbial Fuel Cell (MBMFC)—was developed, fabricated, and tested to evaluate its electrical energy generation. The design focused on balancing microfluidic architecture and wiring procedures with microbial community dynamics to maximize power output and allow for upscaling and thus practical implementation. The testing phase involved experimentation to evaluate the performance of the MBMFC. Microbial feedstock was varied to assess its impact on power generation. The designed MBMFC represents a promising advancement in the field of bioenergy generation. By integrating innovative design principles with advanced fabrication techniques, this study demonstrates a systematic approach to optimizing MFC performance for sustainable and clean energy production.