bluuebunny/crossref_metadata_2025 huggingface dataset:
Classification of Dog Breeds Using Convolutional Neural Network Models and Support Vector Machine
Authors: Cui Ying , Tang Bixia , Wu Gangao , Li Lun , Zhang Xin , Du Zhenglin , Zhao Wenming
Published: November 2024

When classifying breeds of dogs, the accuracy of classification significantly affects breed identification and dog research. Using images to classify dog breeds can improve classification efficiency; however, it is increasingly challenging due to the diversities and similarities among dog breeds. Traditional image classification methods primarily rely on extracting simple geometric features, while current convolutional neural networks (CNNs) are capable of learning high-level semantic features. However, the diversity of dog breeds continues to pose a challenge to classification accuracy. To address this, we developed a model that integrates multiple CNNs with a machine learning method, significantly improving the accuracy of dog images classification. We used the Stanford Dog Dataset, combined image features from four CNN models, filtered the features using principal component analysis (PCA) and gray wolf optimization algorithm (GWO), and then classified the features with support vector machine (SVM). The classification accuracy rate reached 95.24% for 120 breeds and 99.34% for 76 selected breeds, respectively, demonstrating a significant improvement over existing methods using the same Stanford Dog Dataset. It is expected that our proposed method will further serve as a fundamental framework for the accurate classification of a wider range of species.
Targeting the mTOR-Autophagy Axis: Unveiling Therapeutic Potentials in Osteoporosis
Authors: Chen Rongjin , Yang Chenhui , Yang Fei , Yang Ao , Xiao Hefang , Peng Bo , Chen Changshun , Geng Bin , Xia Yayi
Published: November 2024

Osteoporosis (OP) is a widespread age-related disorder marked by decreased bone density and increased fracture risk, presenting a significant public health challenge. Central to the development and progression of OP is the dysregulation of the mechanistic target of the rapamycin (mTOR)-signaling pathway, which plays a critical role in cellular processes including autophagy, growth, and proliferation. The mTOR-autophagy axis is emerging as a promising therapeutic target due to its regulatory capacity in bone metabolism and homeostasis. This review aims to (1) elucidate the role of mTOR signaling in bone metabolism and its dysregulation in OP, (2) explore the interplay between mTOR and autophagy in the context of bone cell activity, and (3) assess the therapeutic potential of targeting the mTOR pathway with modulators as innovative strategies for OP treatment. By examining the interactions among autophagy, mTOR, and OP, including insights from various types of OP and the impact on different bone cells, this review underscores the complexity of mTOR’s role in bone health. Despite advances, significant gaps remain in understanding the detailed mechanisms of mTOR’s effects on autophagy and bone cell function, highlighting the need for comprehensive clinical trials to establish the efficacy and safety of mTOR inhibitors in OP management. Future research directions include clarifying mTOR’s molecular interactions with bone metabolism and investigating the combined benefits of mTOR modulation with other therapeutic approaches. Addressing these challenges is crucial for developing more effective treatments and improving outcomes for individuals with OP, thereby unveiling the therapeutic potentials of targeting the mTOR-autophagy axis in this prevalent disease.
Exosomes from Human Periodontal Ligament Stem Cells Promote Differentiation of Osteoblast-like Cells and Bone Healing in Rat Calvarial Bone
Authors: Albougha Mhd Safwan , Sugii Hideki , Adachi Orie , Mardini Bara , Soeno Serina , Hamano Sayuri , Hasegawa Daigaku , Yoshida Shinichiro , Itoyama Tomohiro , Obata Junko , Maeda Hidefumi
Published: November 2024

Deep caries and severe periodontitis cause bone resorption in periodontal tissue, and severe bone resorption leads to tooth loss. Periodontal ligament stem cells (PDLSCs) are important for the healing of defective periodontal tissue. It is increasingly understood that healing of periodontal tissue is mediated through the secretion of trophic factors, particularly exosomes. This study investigated the effects of exosomes from human PDLSCs (HPDLSCs-Exo) on human osteoblast-like cells in vitro and on the healing of rat calvarial bone defects in vivo. HPDLSCs-Exo were isolated and characterized by their particle shape, size (133 ± 6.4 nm), and expression of surface markers (CD9, CD63, and CD81). In vitro results showed that HPDLSCs-Exo promoted the migration, mineralization, and expression of bone-related genes such as alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP2), osteocalcin (OCN), and osteopontin (OPN) in human osteoblast-like cells. Furthermore, in vivo results showed that more newly formed bone was observed in the HPDLSCs-Exo-treated group than in the non-treated group at the defect sites in rats. These results indicated that HPDLSCs-Exo could promote osteogenesis in vitro and in vivo, and this suggests that HPDLSCs-Exo may be an attractive treatment tool for bone healing in defective periodontal tissue.
Angiotensin II Induces Vascular Endothelial Dysfunction by Promoting Lipid Peroxidation-Mediated Ferroptosis via CD36
Authors: Zhou Qian , Zhang Ying , Shi Wei , Lu Lu , Wei Jianglan , Wang Jinhan , Zhang Hu , Pu Yuepu , Yin Lihong
Published: November 2024

Angiotensin II (Ang II) is an effective vasoconstriction peptide, a major effector molecule of the renin–angiotensin–aldosterone system (RAAS) and one of the important causes of endothelial dysfunction. Ferroptosis is considered to be involved in the occurrence and development of cardiovascular diseases. This study is dedicated to exploring the role and mechanism of Ang II-induced ferroptosis in HUVECs and to finding molecular targets for vascular endothelial injury and dysfunction during the progression of hypertension. In this study, we found that with the increase in exposure concentration, the intracellular ROS content and apoptosis rate increased significantly, the NO release decreased significantly in the medium- and high-concentration groups and the ET-1 content in the high-concentration group increased significantly. The expression of ZO-1 protein was significantly decreased in the high-concentration group. The expression of p-eNOS, VE-cadherin and Occludin protein showed a dose-dependent downward trend, while the ICAM-1 protein showed an upward trend. Ang II caused lipid metabolism disorders in HUVECs, and the PL–PUFAs associated with ferroptosis were significantly increased. In addition, Ang II promoted a significant increase in intracellular free Fe2+ content and MDA and a significant decrease in GSH content. Furthermore, the expression of GPX4, SLC7A11 and SLC3A2 was down-regulated, the expression of ACSL4, LPCAT3 and ALOX15 was up-regulated, and the ratio of p-cPLA2/cPLA2 was increased. After the intervention of ferroptosis inhibitor Fer-1, the injury and dysfunction of HUVECs induced by Ang II were significantly rescued. Immunofluorescence results showed that the expression of CD36 showed a significant increasing trend and was localized in the cytoplasm. Over-expression of CD36 promoted Ang II-induced ferroptosis and endothelial dysfunction. In conclusion, Ang II induces the injury of HUVECs, decreases vascular diastole and endothelial barrier-related molecules, and increases vascular constriction and adhesion-related molecules, which may be related to CD36 and its mediated lipid peroxidation and ferroptosis signals.
Neuroradiological Findings in Cerebral Amyloid Angiopathy with a Particular Consideration of the Boston Criteria 2.0: An Imaging Review
Authors: Jensen-Kondering Ulf , Heß Katharina , Neumann Alexander , Margraf Nils G.
Published: November 2024

In the elderly, cerebral amyloid angiopathy (CAA) is the most common cause for intracranial lobar hemorrhages. CAA is caused by the accumulation of amyloid-β fibrils in cortical and leptomeningeal vessels. In 2022, the Boston Criteria 2.0 became the new diagnostic standard for CAA, following the Modified Boston Criteria of 2010. The diagnostic criteria are a composite of clinical, imaging and histopathological findings. In the latest version of the Boston Criteria, neuroradiological imaging findings were even expanded compared to the previous version. Crucially, the correct application of the diagnostic criteria is necessary to avoid over- and underdiagnosis. The aim of this review is to demonstrate the diagnostic criteria for CAA with an emphasis on typical imaging findings which are part of the Boston Criteria 2.0 and other imaging findings suggestive of CAA.
Activins and Inhibins in Cardiovascular Pathophysiology
Authors: Tang Wenyi , Gu Zhilin , Guo Jiuqi , Lin Mingzhi , Tao Hongqian , Jia Dalin , Jia Pengyu
Published: November 2024

Activins and inhibins, members of the transforming growth factor β (TGFβ) superfamily, were initially recognized for their opposing effects on the secretion of follicle-stimulating hormone. Subsequent research has demonstrated their broader biological roles across various tissue types. Primarily, activins and inhibins function through the classical TGFβ SMAD signaling pathway, but studies suggest that they also act through other pathways, with their specific signaling being complex and context-dependent. Recent research has identified significant roles for activins and inhibins in the cardiovascular system. Their actions in other systems and their signaling pathways show strong correlations with the development and progression of cardiovascular diseases, indicating potential broader roles in the cardiovascular system. This review summarizes the progress in research on the biological functions and mechanisms of activins and inhibins and their signaling pathways in cardiovascular diseases, offering new insights for the prevention and treatment of cardiovascular diseases.
Gut Microbe-Generated Metabolite Trimethylamine-N-Oxide and Ischemic Stroke
Authors: Li Zhen , He Xinyi , Fang Qi , Yin Xulong
Published: November 2024

Trimethylamine-N-oxide (TMAO) is a gut microbiota-derived metabolite, the production of which in vivo is mainly regulated by dietary choices, gut microbiota, and the hepatic enzyme flavin monooxygenase (FMO), while its elimination occurs via the kidneys. The TMAO level is positively correlated with the risk of developing cardiovascular diseases. Recent studies have found that TMAO plays an important role in the development of ischemic stroke. In this review, we describe the relationship between TMAO and ischemic stroke risk factors (hypertension, diabetes, atrial fibrillation, atherosclerosis, thrombosis, etc.), disease risk, severity, prognostic outcomes, and recurrence and discuss the possible mechanisms by which they interact. Importantly, TMAO induces atherosclerosis and thrombosis through lipid metabolism, foam cell formation, endothelial dysfunction (via inflammation, oxidative stress, and pyroptosis), enhanced platelet hyper-reactivity, and the upregulation and activation of vascular endothelial tissue factors. Although the pathogenic mechanisms underlying TMAO’s aggravation of disease severity and its effects on post-stroke neurological recovery and recurrence risk remain unclear, they may involve inflammation, astrocyte function, and pro-inflammatory monocytes. In addition, this paper provides a summary and evaluation of relevant preclinical and clinical studies on interventions regarding the gut-microbiota-dependent TMAO level to provide evidence for the prevention and treatment of ischemic stroke through the gut microbe–TMAO pathway.
Schinus molle Resin Essential Oil as Potent Bioinsecticide Against Tribolium castaneum: Chemical Profile, In Vitro Acetylcholinesterase Inhibition, DFT Calculation and Molecular Docking Analysis
Authors: Baccari Wiem , Saidi Ilyes , Jebnouni Achref , Teka Safa , Osman Sayda , Mansoor Alrasheeday Awatif , Mohamed Nuzaiha , El Oudi Mabrouka , Ben Jannet Hichem
Published: November 2024

Plants offer a bountiful source of natural pest control solutions through their essential oils. This research introduces and analyzes an eco-friendly natural essential oil for red flour beetle control. Therefore, the current study was included to show the chemical profile and the insecticidal efficacy of resin essential oil (REO) and its fractions (F1–3), resulting from chromatographic separation, from the plant Schinus molle against Tribolium castaneum adults. The trunk bark resin essential oil and its fractions’ composition were analyzed by GC-MS. Overall, 33 constituents with 98.3% of the total EO composition were identified. REO and F1–3 displayed impressive repellent properties at a concentration of 0.12 µL/cm2. After 120 min of exposure, repellency ranged from 73.3% to a remarkable 96.7%. They also exhibited noteworthy fumigant properties, with median lethal doses of LD50 = 120.6–160.8 μL/L. The fractions F1 and F3 showed the most notable topical toxicity at a concentration of 10%, with LD50 values of 8.6% and 5.6%, respectively. Fractions F3 and F2 demonstrated the most effective inhibition of acetylcholinesterase (AChE) activity, providing insight into their insecticidal mechanisms. The in silico molecular docking and DFT studies corroborate the results of in vitro tests performed to identify new insecticide products derived from natural sources.
Cardio-Lipotoxicity of Epicardial Adipose Tissue
Authors: Bodenstab Monica L. , Varghese Ron T. , Iacobellis Gianluca
Published: November 2024

Epicardial adipose tissue is a unique visceral adipose tissue depot that plays a crucial role in myocardial metabolism. Epicardial adipose tissue is a major source of energy and free fatty acids for the adjacent myocardium. However, under pathological conditions, epicardial fat can affect the heart through the excessive and abnormal influx of lipids. The cardio-lipotoxicity of the epicardial adipose tissue is complex and involves different pathways, such as increased inflammation, the infiltration of lipid intermediates such as diacylglycerol and ceramides, mitochondrial dysfunction, and oxidative stress, ultimately leading to cardiomyocyte dysfunction and coronary artery ischemia. These changes can contribute to the pathogenesis of various cardio-metabolic diseases including atrial fibrillation, coronary artery disease, heart failure, and obstructive sleep apnea. Hence, the role of the cardio-lipotoxicity of epicardial fat and its clinical implications are discussed in this review.
Methyl Paraben Affects Porcine Oocyte Maturation Through Mitochondrial Dysfunction
Authors: Huang Huimei , Huang Chuman , Li Yinghua , Liang Xingwei , Kim Namhyung , Xu Yongnan
Published: November 2024

Parabens are widely used in various industries, which are including chemical, pharmaceutical, food, cosmetic, and plastic processing industries. Among these, methyl paraben (MP) serves as an antimicrobial preservative in processed foods, pharmaceuticals, and cosmetics, and it is particularly detected in baby care products. Studies indicate that MP functions as an endocrine-disrupting compound with estrogenic properties, negatively affecting mitochondrial bioenergetics and antioxidant activity in testicular germ cells. However, limited information exists regarding studies on the effects of MP in oocytes. The aim of this study was to investigate the specific mechanism and the toxic effects of MP during oocyte maturation cultured in vitro using a porcine oocyte model. The results indicated that MP (50 μM) inhibited oocyte expansion, significantly reducing the expression of expansion-related genes MAPK1 and ERK1, and decreased the first polar body extrusion significantly as well. ATP levels decreased, reactive oxygen species (ROS) levels remained unchanged, and glutathione (GSH) levels decreased significantly, resulting in an elevated ROS/GSH ratio. The expression of antioxidant genes SOD1 and GPX was significantly decreased. Additionally, a significant decrease in levels of mitochondrial production and biosynthesis protein PGC1α+β, whereas levels of antioxidant-related protein Nrf2 and related gene expression were significantly increased. Autophagy protein LC3B and gene expression significantly decreased, and apoptosis assay indicated a significant increase in levels of caspase3 protein and apoptosis-related genes. These results demonstrated the negative effect of MP on oocyte maturation. In conclusion, our findings indicate that MP disrupts redox balance and induces mitochondrial dysfunction during meiosis in porcine oocytes, resulting in the inhibition of meiotic progression. The present study reveals the mechanism underlying the effects of methyl para-hydroxybenzoate on oocyte maturation.
High-Sensitivity C-Reactive Protein Levels in Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD), Metabolic Alcohol-Associated Liver Disease (MetALD), and Alcoholic Liver Disease (ALD) with Metabolic Dysfunction
Authors: Baek Seong-Uk , Yoon Jin-Ha
Published: November 2024

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a recently introduced term for steatotic liver disease (SLD). Although the inflammatory process is central to the pathogenesis of SLD, research investigating the differences in systemic inflammation across various SLD subtypes as well as sex differences is limited. This population-based, cross-sectional study investigated the association between SLD subtypes and high-sensitivity C-reactive protein (hs-CRP) levels among Korean adults (N = 20,141; mean age: 50.8 ± 16.7 years). The participants were classified into five groups that included no SLD, MASLD, metabolic alcohol-associated liver disease (MetALD), alcoholic liver disease with metabolic dysfunction (ALD with MD), and other SLDs. The median (Q1, Q3) value of the hs-CRP level was 0.54 mg/L (0.33, 1.04). Among men, compared to levels in the no SLD group, the MASLD, MetALD, and ALD with MD groups were associated with 41.9% (95% confidence interval [CI]: 35.1–49.1%), 46.8% (95% CI: 35.0–59.6%), and 51.8% (95% CI: 30.0–77.2%) increases in hs-CRP levels, respectively. The association between SLD subtypes and hs-CRP levels was stronger among women, and compared to the levels in the no SLD group, the MASLD, MetALD, and ALD with MD groups were associated with 81.5% (95% CI: 73.6–89.8%), 84.3% (95% CI: 58.1–114.8%), and 98.2% (95% CI: 38.0–184.8%) increases in hs-CRP levels, respectively. In conclusion, our findings indicate a varying profile of systemic inflammation across SLD subtypes, with more pronounced increases in hs-CRP levels in women with SLDs.
Advances in the Regulation of Periostin for Osteoarthritic Cartilage Repair Applications
Authors: Shih Sunny Y. , Grant Michael P. , Epure Laura M. , Alad Muskan , Lerouge Sophie , Huk Olga L. , Bergeron Stephane G. , Zukor David J. , Merle Géraldine , Im Hee-Jeong , Antoniou John , Mwale Fackson
Published: November 2024

Emerging evidence indicates periostin (POSTN) is upregulated in patients with OA, and studies have shown that it can induce the activation of inflammatory cytokines and catabolic enzymes, making it a potential therapeutic target. Link N (LN) is a peptide fragment derived from the link protein and has been demonstrated as an anabolic-like factor and anti-catabolic and anti-inflammatory factors both in vitro and in vivo. This study aims to determine if LN can regulate POSTN expression and function in OA cartilage. Articular cartilage was recovered from donors undergoing total knee replacements to isolate chondrocytes and prepare osteochondral explants. Cells and explants were treated with POSTN and LN (1 and 100 μg) and measured for changes in POSTN expression and various matrix proteins, catabolic and proinflammatory factors, and signaling. To determine the effects of POSTN expression in vivo, a rabbit OA model was used. Immunoprecipitation and in silico modeling were used to determine peptide/POSTN interactions. Western blotting, PCR, and immunohistochemistry demonstrated that LN decreased POSTN expression both in vitro and in vivo. LN was also able to directly inhibit POSTN signaling in OA chondrocytes. In silico docking suggested the direct interaction of LN with POSTN at residues responsible for its oligomerization. Immunoprecipitation experiments confirmed the direct interaction of LN with POSTN and the destabilization of its oligomerization. This study demonstrates the ability of a peptide, LN, to suppress the overexpression and function of POSTN in OA cartilage.
Short-Cycle Therapy with Bictegravir/Emtricitabine/Tenofovir Alafenamide in a Small Cohort of Virally Suppressed People Living with HIV: A Long-Term Follow-Up
Authors: Lanzafame Massimiliano , Lattuada Emanuela , Delama Andrea , Mori Giovanni , Vento Sandro
Published: November 2024

Background: Antiretroviral triple therapy has considerably reduced morbidity and mortality in people living with HIV and is the standard-of-care treatment. However, it is lifelong and linked to long-term side effects and adherence problems. Methods: Here, we report long-term virological and immunological outcome in 12 virally suppressed people on short-cycle therapy with bictegravir/emtricitabine/tenofovir alafenamide administered five days a week (Monday to Friday). Results: All patients, after a long term follow-up, were virally suppressed Conclusions: In the wait for new long-acting antiretroviral drugs and new antiretroviral formulations, short-cycle therapy has proven to be a safe and effective alternative to the standard daily antiretroviral regimen for individuals living with HIV who are virologically suppressed.
Prognostic Value of Circulating Biomarkers of Fibrotic Remodeling in Arrhythmogenic Cardiomyopathy
Authors: Chelko Stephen P.
Published: November 2024

Arrhythmogenic cardiomyopathy (ACM) is a nonischemic, familial heart disease with a high risk of sudden cardiac death (SCD) in the pediatric population and accounts for >20% of SCDs worldwide [...]
Analysis of Circulating Catestatin in Early Pregnancy: A Preliminary Investigation
Authors: Sunjic Lovric Zdenka , Resic Karara Jasminka , Mimica Bianka , Kumric Marko , Supe-Domic Daniela , Santic Roko , Bozic Josko
Published: November 2024

Background: During pregnancy, significant cardiovascular changes occur to accommodate fetal growth, and catestatin may play a role in these changes. Evidence suggests that catestatin, a pleiotropic sympathoinhibitory peptide, is involved in multiple cardiovascular pathologies, including hypertensive disorders. The objective of this study was to compare serum catestatin levels between first-trimester pregnant women and non-pregnant women, aiming to investigate catestatin’s role in blood pressure regulation during early pregnancy. Methods: This cross-sectional study included 72 first-trimester pregnant women and 57 age-matched non-pregnant controls, all without known cardiovascular or metabolic disorders. Results: Serum catestatin concentrations were significantly higher in pregnant women compared to controls (12.4 (9.9–21.2) ng/mL vs. 7.1 (4.5–10.9) ng/mL, p < 0.001). However, there was no significant difference in serum catestatin levels between those with a normal and abnormal uterine artery pulsatility index (17.8 (8.3-22.3) ng/mL vs. 12.5 (9.9–22.4) ng/mL, p = 0.962). Similarly, catestatin concentrations did not significantly differ between primiparous and multiparous women (14.0 (11.5–22.4) ng/mL vs. 10.7 (8.8–19.0) ng/mL). A positive correlation was observed between systolic blood pressure and serum catestatin levels in the control group (r = 0.335, p = 0.011) but not in pregnant women. Conclusions: Research on catestatin in pregnancy is still in its early stages, necessitating further studies to fully elucidate its roles and potential therapeutic applications.
Visual Cortical Function Changes After Perceptual Learning with Dichoptic Attention Tasks in Adults with Amblyopia: A Case Study Evaluated Using fMRI
Authors: Hou Chuan , Zhou Zhangziyi , Uner Ismet Joan , Nicholas Spero C.
Published: November 2024

Background: Amblyopia is a neurodevelopmental disorder of vision, commonly caused by strabismus or anisometropia during early childhood. While studies demonstrated that perceptual learning improves visual acuity and stereopsis in adults with amblyopia, accompanying changes in visual cortical function remain unclear. Methods: We measured functional magnetic resonance imaging (fMRI) responses before and after perceptual learning in seven adults with amblyopia. Our learning tasks involved dichoptic high-attention-demand tasks that avoided V1 function-related tasks and required high-level cortical functions (e.g., intraparietal sulcus) to train the amblyopic eye. Results: Perceptual learning induced low-level visual cortical function changes, which were strongly associated with the etiology of amblyopia and visual function improvements. Anisometropic amblyopes showed functional improvements across all regions of interest (ROIs: V1, V2, V3, V3A, and hV4), along with improvements in visual acuity and stereoacuity. In contrast, strabismic amblyopes showed robust improvements in visual cortical functions only in individuals who experienced significant gains in visual acuity and stereoacuity. Notably, improvements in V1 functions were significantly correlated with the magnitude of visual acuity and stereoacuity improvements when combining both anisometropic and strabismic amblyopes. Conclusions: Our findings provide evidence that learning occurs in both high-level and low-level cortical processes. Our study suggests that early intervention to correct eye alignment (e.g., strabismus surgery) is critical for restoring both visual and cortical functions in strabismic amblyopia.
Sensitivity of Spiking Neural Networks Due to Input Perturbation
Authors: Zhu Haoran , Zeng Xiaoqin , Zou Yang , Zhou Jinfeng
Published: November 2024

Background: To investigate the behavior of spiking neural networks (SNNs), the sensitivity of input perturbation serves as an effective metric for assessing the influence on the network output. However, existing methods fall short in evaluating the sensitivity of SNNs featuring biologically plausible leaky integrate-and-fire (LIF) neurons due to the intricate neuronal dynamics during the feedforward process. Methods: This paper first defines the sensitivity of a temporal-coded spiking neuron (SN) as the deviation between the perturbed and unperturbed output under a given input perturbation with respect to overall inputs. Then, the sensitivity algorithm of an entire SNN is derived iteratively from the sensitivity of each individual neuron. Instead of using the actual firing time, the desired firing time is employed to derive a more precise analytical expression of the sensitivity. Moreover, the expectation of the membrane potential difference is utilized to quantify the magnitude of the input deviation. Results/Conclusions: The theoretical results achieved with the proposed algorithm are in reasonable agreement with the simulation results obtained with extensive input data. The sensitivity also varies monotonically with changes in other parameters, except for the number of time steps, providing valuable insights for choosing appropriate values to construct the network. Nevertheless, the sensitivity exhibits a piecewise decreasing tendency with respect to the number of time steps, with the length and starting point of each piece contingent upon the specific parameter values of the neuron.
The Effectiveness of Motor Imagery in Balance and Functional Status of Older People with Early-Stage Dementia
Authors: Christakou Anna , Bouzineki Christina , Pavlou Marousa , Stranjalis George , Sakellari Vasiliki
Published: November 2024

Background/Objectives: Dementia involves the loss of cognitive abilities and impairs functional abilities in daily life. In motor imagery (MI) techniques, motor acts are mentally rehearsed without any overt body movements. The purpose of the randomized controlled trial was to examine the effects of MI on the motor function of older adults with dementia. Methods: Overall, 160 participants (43 men, 117 women, MMSE M = 23.20, SD = 0.15) from an Athens Day Care Center of the Alzheimer Association were randomized to (a) the MI and exercise group (experimental group) (n = 55), (b) the only exercise group (1st control group) (n = 52) and (c) the neither MI nor exercise group (2nd control group) (n = 53). The exercise session comprised 24 physiotherapy exercise sessions, lasting 45 min each, twice a week for 12 weeks. The exercises were selected from the Otago Exercise Program. Three assessments were performed: (a) one week prior to the program, (b) at one and a half months and (c) after the program. The experimental group performed a 30-minute MI with exercise program content after the end of every physiotherapy exercise session. The Multidirectional Reach Test, Five Times Sit-to-Stand Test (FTSST), Timed Up and Go test (TUG), Functional Gait Assessment (FGA) and Berg Balance Scale (BBS) were used to assess participants’ balance and functional status. Results: In the intention to treat analysis (18 participants dropped out), the 3 × 3 repeated measures ANOVA indicated statistically significant results between the three groups on (a) the TUG (F = 3.06, df (2), p = 0.04), (b) the FTSST (F = 3.00, df (2), p = 0.05), (c) the forward direction test (F = 4.14 df (2), p = 0.02), the lateral right and the lateral left direction tests (F = 3.90, df (2), p = 0.02 and F = 7.87, df (2), p = 0.00, respectively), and (d) the FGA (F = 4.35, df (2), p = 0.01). The Friedman test showed significant statistical significant differences among the three groups for BBS (X2 = 7.62, df = 2, p = 0.22), and an effect size of partial η2 coefficient for F-tests was found. Post hoc comparisons using a Bonferroni test for ANOVA and Wilcoxon test for Friedman indicated that the mean scores for the experimental group and the 1st control were significantly better than the 2rd control group in many dependent variables. Conclusions: The study showed a positive effect of MI on balance and the functional status of older adults with early stages of dementia with possible beneficial effects on maintaining independence and reducing physical decline.
The Effects of Post-Warm-Up Active and Passive Rest Periods on a Vigilance Task in Karate Athletes
Authors: Silva Rui Miguel , González-Fernández Francisco , Rusillo-Magdaleno Alba , Loureiro Vânia , Pires Dinis , Ferreira Filipe , Silva Ana Filipa
Published: November 2024

This study aimed to analyze how active versus passive rest periods after a warm-up influence performance in psychomotor vigilance tasks (PVT). Twenty amateur karate athletes participated in a randomized cross-over study consisting of two sessions with either a 20 min active rest involving kata techniques or passive rest. PVT was administered before and after these conditions to assess the changes in reaction time. The results revealed that the active rest condition significantly improved reaction times compared to both the passive rest condition (F(1,31) = 5.34, p = 0.03, η2 partial = 0.14) and control condition (F(1,31) = 5.49, p = 0.02, η2 partial = 0.15). No significant time-on-task effects were observed, F(4,120) = 2.31, p = 0.06, and there were no significant interactions between effort condition and time-on-task, F(4,120) = 1.89, p = 0.11). Participating in an active rest period post-warm-up improves cognitive performance in karate athletes, as evidenced by quicker reaction times in the PVT. This finding supports the use of active rest strategies (involving kata techniques) to maintain and improve cognitive readiness in young karate athletes.
Analysis of Gravel Migration Patterns During Vibration Rolling and Their Impact on GCL Performance Based on DEM
Authors: Huang Hu , Chen Feihao , Qiu Qingming , Li Ruihang , Guo Lixia
Published: November 2024

In this study, a multilayer composite rolling model consisting of a rolling wheel, a protective layer, a GCL, and a support layer was constructed by the discrete element method (DEM). Soil compaction and gravel migration, and their effects on the GCL, were analyzed from a fine viewpoint, and three key indexes for the safety assessment of the GCL were proposed: local elongation, gravel embedment value, and bentonite allotment number. The results show that the soil porosity and cumulative settlement do not decrease all the time with the number of rolling passes, and there exists an optimal number of rolling passes during the rolling process; the protective layer of gravel soil moves more frequently than the support layer; and the nearly rectangular and nearly elliptical gravels are more likely to rotate. The maximum local elongation of the GCL was 3.79% during the lapping process, and all gravels in contact with the upper boundary of the GCL extruded the GCL to varying degrees during the lapping process. The distribution of bentonite particles is closely related to the contact mode between gravel and GCL.
Building Condition Auditing (BCA)—Improving Auditability—Reducing Ambiguity
Authors: West Jye , Siddhpura Milind , Evangelista Ana , Haddad Assed
Published: November 2024

BCA methodically assesses the state of a building’s deterioration to support Maintenance, Safety, Function, and Compliance purposes. Originally used to assist in identifying urgent repair requirements, it has evolved and become one of the most used tools for assessing a building’s outstanding maintenance liability when a building is transacted or acquired. Nevertheless, current practices involve several conflicts; for example, high costs are associated with inspections, inconsistent building component registers, and ambiguity and consistency regarding reporting parameters, all of which lead to compounding errors that reduce reliability. To address these gaps, the current research, involving one hundred and eighteen (118) active facilities managers and asset inspectors, suggests the development of an extension of the deterioration scale (0–7) and methodologies to reduce errors and ambiguity. Furthermore, it suggests using weighted indices to focus on crucial building components, thus improving condition assessment. As was found, these tools improve the accuracy of BCA, facilitate better management of the asset’s life cycle, and provide support in decision-making. This study adds consistency, limits subjectivity, and provides a framework applicable to different building types, assisting future management for sustainability. It, therefore, stands to serve the field by providing detailed and concise best practices for conducting condition audits on built assets.
Blockchain-Based Responsibility Management Framework for Smart City Building Information Modeling Projects Using Non-Fungible Tokens
Authors: Bai Hao , Li Zushun , Chen Keyu , Li Xiongwei
Published: November 2024

In the context of digital construction, responsibility management in smart city building information modeling (BIM) projects spans the entire building lifecycle. The involvement of numerous BIM designers in project management and frequent data exchanges pose significant challenges for the traceability, immutability, and responsibility attribution of BIM models. To address these issues, this study proposes a blockchain-based responsibility management and collaboration framework for BIM projects using non-fungible tokens (NFTs), aiming to enhance the management of responsibilities and accountability in BIM projects. This research adopts a design science methodology, strictly adhering to scientific research procedures to ensure rigor. First, NFTs based on blockchain technology were developed to generate corresponding digital signatures for BIM model files. This approach ensures that each BIM model file has a unique digital identity, enhancing transparency and traceability in responsibility management. Next, the interplanetary file system (IPFS) was used to generate digital fingerprints, with the content identifier generated by IPFS uploaded to the blockchain to ensure the immutability of BIM model files. This method guarantees the integrity and security of BIM model files throughout their lifecycle. Finally, the proposed methods were validated through a blockchain network. The experimental results indicate that the proposed framework is theoretically highly feasible and demonstrates good applicability and efficiency in practical production. The constructed blockchain network meets the actual needs of responsibility management in smart city BIM projects, enhancing the transparency and reliability of project management.
Numerical Simulation Study of Bearing Characteristics of Large-Diameter Flexible Piles Under Complex Loads
Authors: Yang Xueying , Gong Weiming , Yin Qian
Published: November 2024

The majority of the existing calculation methods for determining the ultimate bearing capacity of steel-pipe piles using Chinese criteria are designed for piles with diameters smaller than 2 m. To investigate the bearing capacity of flexible steel-pipe piles with diameters larger than 2 m under combined loading conditions, reveal nonlinear interactions between vertical and horizontal loads, and propose bearing capacity envelopes, in this paper, a numerical method was used to study the bearing capacity of a flexible pile with a diameter of 2.8 m and an embedment length of 72 m under vertical and horizontal loading conditions. First, a numerical model was developed and calibrated using field test results. Then, the effects of vertical pressure on horizontal capacity and lateral force on vertical capacity and uplift capacity of the pile were analyzed. The results indicate that vertical pressure at the top of the pile can nonlinearly reduce its horizontal capacity, but this pressure initially has a slight positive effect on the horizontal bearing capacity before causing a rapid decrease. Conversely, horizontal force negatively impacts both the compressive and uplift bearing capacities of the pile. Finally, depending on the above results, bearing capacity envelopes for piles subjected to vertical and horizontal loads were proposed.
Assessment of Architectural Typologies and Comparative Analysis of Defensive Rammed Earth Dwellings in the Fujian Region, China
Authors: Lin Xiuhong , Zhang Yukun , Wu Yilin , Yang Yingqian
Published: November 2024

Tulou, Tubao, and Zhailu all belong to the same vernacular architectural defensive rammed earth dwellings in Fujian; however, because of the similarity of their earthen defensive functions and fortress architectural systems, they are easily confused by scholars and there is still a gap in distinguishing the in-depth differences between the three. For the first time, this article develops a proper architectural typology with a comparative analysis of five aspects (origin and prototype, plan and type, building function and settlement typology, structure type and construction technology, and ethical concept and settlement model) to distinguish their architectural features. We find that they have different architectural prototypes and plan types. Tubao is a defensive dwelling with a residential function, Tulou is a defensive dwelling with a residential function, and Zhailu is a dwelling that combines living and defense. They have six different structural types. Although Tulou has the simplest structure and the thinnest rammed earth wall, its construction technology is the best. Tubao and Zhailu have more clearly defined architectural levels than Tulou, and Tubao’s hierarchy is the most significant. Therefore, we prove that despite their similarities, they are different types of buildings. This study helps to provide a methodological guide for identifying other, similar buildings and serves as a manual for the restoration and sustainable development of defensive rammed earth dwelling.
Full-Scale Experimental Study on Influence of Smoke on Pedestrian Movement During Evacuation in Road Tunnel
Authors: Schmidt-Polończyk Natalia
Published: November 2024

The article presents the results of experimental studies of evacuation of 50 people from a road tunnel in various smoke conditions. Calculations of total evacuation times, pre-movement times and movement speeds were carried out and the impact of smoke on the speed of movement was analyzed. The pre-movement times, the alarm realization and response times for the subsequent experiments (1, 2 and 3) were 36, 7 and 5 s, respectively. The total evacuation times for 3 experiments were 340, 301 and 215 s. It has been shown that the speed of movement in smoke depends not only on the density of smoke, but also on the very attitude of the experiment participants and knowledge of the tunnel. It has also been shown that the adverse impact of low visibility on the evacuation time and movement speed is as important as the motivation of the evacuees and the effect of learning. In order to collect the observations of the participants, as well as assess potential aspects which might have influenced the process of evacuation, a survey was conducted after both experiments. The answers show that the two main reasons that prompted the evacuation were smoke in the tunnel and the fire drill.
Cytogenomic Characterization of Murine Neuroblastoma Cell Line Neuro-2a and Its Two Derivatives Neuro-2a TR-Alpha and Neuro-2a TR-Beta
Authors: Hergenhahn Lioba , Padutsch Niklas , Azawi Shaymaa , Weiskirchen Ralf , Liehr Thomas , Rinčic Martina
Published: November 2024

Background: The Neuro-2a cell line, derived from a murine neuroblastoma (NB), was established as early as 1969 and originates from a transplantable tumor that arose spontaneously in an A/Jax male mouse in 1940. Since then, it has been applied in over 10,000 studies and is used by the World Organization for Animal Health for the routine diagnosis of rabies. Surprisingly, however, Neuro-2a has never been genetically characterized in detail; this study fills that gap. Methods: The Neuro-2a cell line and two of its derivatives, Neuro-2a TR-alpha and Neuro-2a TR-beta, were analyzed for their chromosomal constitution using molecular cytogenetic approaches. Array comparative genomic hybridization was performed to characterize copy number alterations. Results: Neuro-2A has a hyper-tetraploid karyotype with 70 to 97 chromosomes per cell, and the karyotypes of its two examined derivatives were quite similar. Neither of them had a Y-chromosome. The complex karyotype of Neuro-2a includes mitotically stable dicentres, neocentrics, and complex rearrangements resembling chromothripsis events. Although no amplification of euchromatin or oncogenes was detected, there are five derivative chromosomes with the amplification of centromere-near heterochromatic material and 1–5 additional derivatives consisting only of such material. Conclusions: Since satellite DNA amplification has recently been found in advanced human tumors, this finding may be the corresponding equivalent in mice. An in silico translation of the obtained results into the human genome indicated that Neuro-2A is suitable as a model for advanced human NB.
Validation of a Spectral Flow Cytometry Single-Tube Panel for the Clinical Diagnosis and Follow-Up of Children and Adolescents with B-Cell Acute Lymphoblastic Leukemia
Authors: García-Aguilera Gonzalo , Castillo-Robleda Ana , Sanz Alejandro , Ramírez Manuel
Published: November 2024

The ability of flow cytometry to identify and quantify the presence of cell populations defined by their expression profile of specific markers has made this technique a powerful and routinary tool in clinical diagnostic practice. Specifically in the field of hematological malignancies, flow cytometry allows the identification of the correct type and lineage of each patient’s disease and also sensitively quantifies the presence of the disease at precise moments during treatment, that is, levels of measurable residual disease (MRD). The quantification of MRD by flow cytometry has allowed the adaptation of tailored therapies to patients, contributing to the improvement of the results of the different protocols in recent decades. In this context, our objective in the present work was to evaluate the potential impact that spectral flow cytometry can provide compared to conventional cytometry, which is the one usually used in clinics. We present here a comparative study of both technologies, spectral versus conventional flow cytometry, in primary samples corresponding to the diagnosis and follow-up of children and adolescents with acute lymphoblastic leukemia. Our initial experience demonstrates the feasibility of incorporating spectral flow cytometry into the routine workflow of a reference laboratory.
Animal Models of Febrile Seizures: Limitations and Recent Advances in the Field
Authors: Griflyuk Alexandra V. , Postnikova Tatyana Y. , Zaitsev Aleksey V.
Published: November 2024

Febrile seizures (FSs) are defined as seizures occurring in children aged 6 months to 5 years with a background of elevated body temperature. It is one of the most common neurological disorders of childhood, emphasizing the importance of understanding the causes of FSs and their impact on the developing nervous system. However, there are significant limitations to the technologies currently available for studying the etiology and pathophysiology of seizures in humans. It is currently not possible to adequately capture the subtle molecular and structural rearrangements of the nervous system that can occur after seizures in humans. The use of animal models can be invaluable for these purposes. The most commonly used models in modern research are hyperthermic models in rats and mice aged 10–12 days. While these models can reproduce many of the characteristics of FSs, they have certain limitations. This review outlines the key considerations when working with models of FSs, provides an overview of current approaches to producing seizures in different model subjects, and presents a summary of key findings regarding morphological and functional changes in the brain and behavioral alterations that have been identified in studies using animal models of FSs.
Transcription Factor Deformed Wings Is an Atg8a-Interacting Protein That Regulates Autophagy
Authors: Kołodziej Marta , Tsapras Panagiotis , Cameron Alexander D. , Nezis Ioannis P.
Published: November 2024

LC3 (microtubule-associated protein 1 light chain 3, called Atg8 in yeast and Drosophila) is one of the most well-studied autophagy-related proteins. LC3 controls the selectivity of autophagic degradation by interacting with LIR (LC3-interacting region) motifs also known as AIM (Atg8-interacting motifs) on selective autophagy receptors that carry cargo for degradation. Although the function of Atg8 family proteins is primarily cytoplasmic, they are also enriched in the nucleus. Despite the accumulating evidence indicating the presence of Atg8 proteins in the nucleus, the mechanisms by which they are targeted to the nucleus, their interactions with nuclear components, and their nuclear role in remain poorly understood. Here, we used yeast two-hybrid screening, and we identified transcription factor Deformed wings (Dwg) as an Atg8a-interacting protein in Drosophila. Dwg-Atg8a interaction is LIR motif-dependent. We have created Dwg Y129A/I132A LIR mutant flies and shown that they exhibit elevated autophagy, improved resistance to oxidative stress, and starvation. Our results provide novel insights into the transcriptional regulation of autophagy in Drosophila.