bluuebunny/crossref_metadata_2025 huggingface dataset:
Leaching of Zinc for Subsequent Recovery by Hydrometallurgical Techniques from Electric Arc Furnace Dusts and Utilisation of the Leaching Process Residues for Ceramic Materials for Construction Purposes
Authors: Terrones-Saeta Juan María , Suárez-Macías Jorge , Moreno-López Evaristo Rafael , Corpas-Iglesias Francisco Antonio
Published: October 2021

Steel is one of the most widely used materials in the past and today. Various techniques are used to recycle this material, including the electric arc furnace. This process has several advantages, but it also has a major disadvantage, namely, the generation of waste such as electric arc furnace dusts. Electric arc furnace dusts are classified as hazardous waste due to their high percentage of heavy metals, including zinc. Consequently, in the present research, the leaching of zinc for recovery with sulfuric acid solutions at ambient temperature and atmospheric pressure is evaluated, as well as the reuse of the leaching process residue as a raw material for ceramic materials. The sulfuric acid solutions were 0.125, 0.25, 0.5, and 1 molar, using clay for ceramic conforming and percentages of the leaching residue from 0–50%. The results showed that the optimum solution was 1 molar sulfuric acid, recovering all the zinc in the sample in 36 h. Furthermore, it was found that the clay-conformed ceramics with less than 40% leaching residue showed acceptable physical and mechanical properties according to standards. Therefore, this research develops a new environmental hydrometallurgy in which metallic elements of interest are valorized and the production of waste is avoided, reducing the deposition of hazardous waste in landfills and the extraction of raw materials for the manufacture of construction materials.
Effect of Strain Rate and Extrinsic SIZE Effect on Micro-Mechanical Properties of Zr-Based Bulk Metallic Glass
Authors: Tabbakh Thamer , Kurdi Abdulaziz , Basak Animesh Kumar
Published: October 2021

In this study, the mechanical properties and deformation features of Zr-based bulk metallic glass (BMG) are investigated at micro-scale via in situ micro-pillar compression. Furthermore, the effects of the strain rate and micro-pillar diameter on respective stress–strain curves are investigated. Together with the mechanical properties, such unique in situ micro-pillar compression techniques provide physical status to the micro-pillars, referring to the instances of stress–strain curves. It is noted that the effect of the strain rate on the stress–strain behaviour of the BMG diminishes with increasing micro-pillar diameter. In contrast, yield and ultimate compressive strength increase with increasing micro-pillar diameter, up to 4 µm. The deformation details after compression, as a result of conformed mechanical loading, are analysed by SEM and TEM. As evident from electron microscopy investigation, the plastic deformation is evidenced by the presence of multiple slip/shear bands, acting as load accommodation mechanisms in the course of mechanical loading together and resemble local plastic flow (ductile in nature) between two shear plans.
Strengthening Mechanisms in Carbon Nanotubes Reinforced Metal Matrix Composites: A Review
Authors: Carneiro Íris , Simões Sónia
Published: October 2021

Carbon nanotubes (CNTs)-reinforced metal matrix composites are very attractive advanced nanocomposites due to their potential unusual combination of excellent properties. These nanocomposites can be produced by several techniques, the most reported being powder metallurgy, electrochemical routes, and stir or ultrasonic casting. However, the final mechanical properties are often lower than expected. This can be attributed to a lack of understanding concerning the strengthening mechanisms that act to improve the mechanical properties of the metal matrix via the presence of the CNTs. The dispersion of the CNTs is the main challenge in the production of the nanocomposites, and is independent of the production technique used. This review describes the strengthening mechanism that act in CNT-reinforced metal matrix nanocomposites, such as the load transfer, grain refinement or texture strengthening, second phase, and strain hardening. However, other mechanisms can occur, such as solid solution strengthening, and these depend on the metal matrix used to produce the nanocomposites. Different metallic matrices and different production techniques are described to evaluate their influence on the reinforcement of these nanocomposites.
Evaluation of Bioactive Metabolites and Antioxidant-Rich Extracts of Amaranths with Possible Role in Pancreatic Lipase Interaction: In Silico and In Vitro Studies
Authors: Chaturvedi Swati , Gupta Promila
Published: October 2021

Fat/carbohydrate-rich diet consumption or elevated secretion of pancreatic lipase (PL) in pancreatic injury results in increased fat digestion and storage. Several metabolites in plant-based diets can help achieve the requirements of nutrition and fitness together. Presently, nutritional metabolites from Amaranthus tricolor, A. viridis, and Achyranthes aspera were assessed and predicted for daily intake. The volatile-metabolite profiling of their extracts using GC-MS revealed various antioxidant and bioactive components. The implication of these specialized components and antioxidant-rich extracts (EC50 free radical scavenging: 34.1 ± 1.5 to 166.3 ± 14.2 µg/mL; FRAP values: 12.1 ± 1.0 to 34.0 ± 2.0 µg Trolox Equivalent/mg) in lipolysis regulation by means of interaction with PL was checked by in silico docking (Betahistine and vitamins: ΔGbind −2.3 to −4.4 kcal/mol) and in vitro fluorescence quenching. Out of the various compounds and extracts tested, Betahistine, ATRA and AVLA showed better quenching the PL fluorescence. The identification of potential extracts as source of functional components contributing to nutrition and fat regulation can be improved through such study.
Impact of Exercise and Detraining during Childhood on Brown Adipose Tissue Whitening in Obesity
Authors: Takaishi Kaho , Oshima Takaya , Eto Hikari , Nishihira Misuzu , Nguyen Son Tien , Ochi Ryosuke , Fujita Naoto , Urakawa Susumu
Published: October 2021

This study aimed to investigate the influence of childhood exercise and detraining on brown adipose tissue (BAT) whitening in obesity. Four-week-old male Long-Evans Tokushima Otsuka (LETO) rats (n = 9) and Otsuka Long-Evans Tokushima Fatty (OLETF) rats (n = 24) were used as non-obese and obese animals, respectively. OLETF rats were divided into non-exercise sedentary (n = 9) and exercise groups. OLETF rats in the exercise group were further divided into subgroups according to the exercise period—exercise from 10- to 12-weeks-old (n = 6); and exercise from 4- to 6-weeks-old, and detraining from 6- to 12-weeks-old (n = 9). At 12-weeks-old, immediately after exercise period, BAT whitening in OLETF rats was inhibited by exercise despite the fact that hypertrophy was not caused in the plantaris muscle. However, the effectiveness was attenuated during the detraining period. Histological BAT whitening and downregulation of uncoupling protein-1 (UCP-1) were found in non-exercise sedentary OLETF rats at 12-weeks-old. The downregulation was not inhibited, even though exercise histologically inhibited BAT whitening in OLETF rats. Childhood exercise decreased BAT whitening in obesity. Detraining attenuated the inhibition of BAT whitening. These results suggest that regular exercise is needed to improve BAT whitening and downregulation of UCP-1 in obesity.
Analytical Methodology for a Metabolome Atlas of Goat’s Plasma, Milk and Feces Using 1H-NMR and UHPLC-HRMS
Authors: Martias Cécile , Gatien Julie , Roch Léa , Baroukh Nadine , Mavel Sylvie , Lefèvre Antoine , Montigny Frédéric , Schibler Laurent , Emond Patrick , Nadal-Desbarats Lydie
Published: October 2021

Metabolomics has been increasingly used in animal and food sciences. Animal health is one of the most important factor that can also alter animal integrity and welfare. Some studies have already investigated the link between health and metabolic profile of dairy animals. These studies in metabolomics often consider a single type of sample using a single analytical platform (nuclear magnetic resonance or mass spectrometry). Only few studies with multi-platform approaches are also used with a single or a multi type of sample, but they mainly consider dairy cows’ metabolome although dairy goats present similar diseases, that it could be interesting to detect early to preserve animal health and milk production. This study aims to create a metabolic atlas of goat plasma, milk and feces, based on healthy animals. Our study describes a standard operating procedure for three goat matrices: blood plasma, milk, and feces using multiple platforms (NMR (1H), UHPLC (RP)-MS and UHPLC (HILIC)-MS) that follows a unique sample preparation procedure for each sample type to be analyzed on multi-platforms basis. Our method was evaluated for its robustness and allowed a better characterization of goat metabolic profile in healthy conditions.
Apolipoprotein B and Cardiovascular Disease: Biomarker and Potential Therapeutic Target
Authors: Behbodikhah Jennifer , Ahmed Saba , Elyasi Ailin , Kasselman Lora J. , De Leon Joshua , Glass Amy D. , Reiss Allison B.
Published: October 2021

Apolipoprotein (apo) B, the critical structural protein of the atherogenic lipoproteins, has two major isoforms: apoB48 and apoB100. ApoB48 is found in chylomicrons and chylomicron remnants with one apoB48 molecule per chylomicron particle. Similarly, a single apoB100 molecule is contained per particle of very-low-density lipoprotein (VLDL), intermediate density lipoprotein, LDL and lipoprotein(a). This unique one apoB per particle ratio makes plasma apoB concentration a direct measure of the number of circulating atherogenic lipoproteins. ApoB levels indicate the atherogenic particle concentration independent of the particle cholesterol content, which is variable. While LDL, the major cholesterol-carrying serum lipoprotein, is the primary therapeutic target for management and prevention of atherosclerotic cardiovascular disease, there is strong evidence that apoB is a more accurate indicator of cardiovascular risk than either total cholesterol or LDL cholesterol. This review examines multiple aspects of apoB structure and function, with a focus on the controversy over use of apoB as a therapeutic target in clinical practice. Ongoing coronary artery disease residual risk, despite lipid-lowering treatment, has left patients and clinicians with unsatisfactory options for monitoring cardiovascular health. At the present time, the substitution of apoB for LDL-C in cardiovascular disease prevention guidelines has been deemed unjustified, but discussions continue.
Space Detumbling Robot Arm Deployment Path Planning Based on Bi-FMT* Algorithm
Authors: Chen Ning , Zhang Yasheng , Cheng Wenhua
Published: October 2021

In order to avoid damage to service satellites and targets during space missions and improve safety and reliability, it is necessary to study how to eliminate or reduce the rotation of targets. This paper focused on a space detumbling robot and studied the space detumbling robot dynamics and robot arm deployment path planning. Firstly, a certain space detumbling robot with a ‘platform + manipulator + end effector’ configuration is proposed. By considering the end effector as a translational joint, the entire space detumbling robot is equivalent to a link system containing six rotating joints and three translational joints, and the detailed derivation process of the kinematic and dynamic model is presented. Then, ADAMS and MATLAB were used to simulate the model, and the MATLAB results were compared with the ADAMS results to verify the correctness of the model. After that, the robot arm deployment problem was analyzed in detail from the aspects of problem description, constraint analysis and algorithm implementation. An algorithm of robot arm deployment path planning based on the Bi-FMT* algorithm is proposed, and the effectiveness of the algorithm is verified by simulation.
The Characteristics of Intestinal Bacterial Community in Three Omnivorous Fishes and Their Interaction with Microbiota from Habitats
Authors: Bi Sheng , Lai Han , Guo Dingli , Liu Xuange , Wang Gongpei , Chen Xiaoli , Liu Shuang , Yi Huadong , Su Yuqin , Li Guifeng
Published: October 2021

Artificial fishery habitats have been extensively used for fishery resource protection and water habitat restoration, and they could attract a large number of omnivorous fishes to gather together. This study intended to reveal the relationship between bacterial communities in the habitats (water and sediment) and intestines of omnivorous fishes (Oreochromis mossambicus, Toxabramis houdemeri and Hemiculter leucisculus). Therefore, we investigated the bacterial communities of samples collected from intestines, water, and sediments in artificial fishery habitats via 16S rRNA metabarcoding high-throughput sequencing technology. The results showed that there were significant differences in the composition, core indicators, diversity and prediction functions in water, sediments, and intestinal microbial communities of the three omnivorous fish. The microbial diversities were significantly higher in habitats than in intestines. The analysis of similarity (ANOSIM) and nonmetric multidimensional scaling (NMDS) results indicated that the intestine microbial communities (T. houdemeri and H. leucisculus) were more similar to the water microbiota, but the intestine microbial communities (O. mossambicus) were more similar to the sediments. Source tracking analysis also confirmed that the contribution of habitat characteristics to omnivorous fish intestinal microorganisms was different; the sediment had a greater contribution than water to the intestinal microbiota of O. mossambicus, which was consistent with their benthic habit. Moreover, the functional prediction results showed that there were unique core indicators and functions between the bacterial community of habitats and intestines. Altogether, these results can enhance our understanding of the bacterial composition and functions about omnivorous fish intestines and their living with habitats, which have provided new information for the ecological benefits of artificial fishery habitats from the perspective of bacterial ecology and contributed to apply artificial fishery habitats in more rivers.
New Exopolysaccharides Produced by Bacillus licheniformis 24 Display Substrate-Dependent Content and Antioxidant Activity
Authors: Petrova Penka , Arsov Alexander , Ivanov Ivan , Tsigoriyna Lidia , Petrov Kaloyan
Published: October 2021

Bacillus licheniformis is a soil bacterium with many industrial applications. In addition to enzymes, platform chemicals, antibiotics and phytohormones, the species produces exopolysaccharides (EPSs) of various biological activities. This study revealed that Bulgarian isolate B. licheniformis 24 produced EPSs consisting of galactose, glucose and mannose with substrate-dependent ratio. From glucose, B. licheniformis 24 secreted EPS1, consisting of 54% galactose, 39% glucose and 7% mannose. From fructose, the strain formed EPS2, containing 51% glucose, 30% mannose and 19% galactose. Batch cultivation in flasks yielded 2.2–2.6 g/L EPS1 and 1.90–2.11 g/L EPS2. Four to five times higher yields of EPS were obtained from both substrates during batch and fed-batch processes in a fermenter at 37.8 °C, pH 6.2 and aeration 3.68 vvm. The batch process with 200 g/L of starting substrates received 9.64 g/L EPS1 and 6.29 g/L EPS2, reaching maximum values at the 33rd and 24th h, respectively. Fed-batch fermentation resulted in the highest yields, 12.61 g/L EPS1 and 7.03 g/L EPS2. In all processes, EPSs were produced only in the exponential growth phase. Both EPSs exhibited antioxidant activity, but EPS2 was much more potent in this regard, reaching 811 μM Vitamin C Equivalent Antioxidant Capacity (versus 135 μM for EPS1). EPS1 displayed antibacterial activity against a non-O1 strain of Vibrio cholerae.
Evaluation of Tetracycline Resistance and Determination of the Tentative Microbiological Cutoff Values in Lactic Acid Bacterial Species
Authors: Ma Qingqing , Pei Zhangming , Fang Zhifeng , Wang Hongchao , Zhu Jinlin , Lee Yuan-kun , Zhang Hao , Zhao Jianxin , Lu Wenwei , Chen Wei
Published: October 2021

Lactic acid bacteria (LAB) are widely used as probiotics in the food industry owing to their beneficial effects on human health. However, numerous antibiotic resistance genes have been found in LAB strains, especially tetracycline resistance genes. Notably, the potential transferability of these genes poses safety risks. To comprehensively evaluate tetracycline resistance in LAB, we determined the tetracycline susceptibility patterns of 478 LAB strains belonging to four genera and eight species. By comparing phenotypes with genotypes based on genome-wide annotations, five tetracycline resistance genes, tet(M), tet(W/N/W), tet(L), tet(S), and tet(45), were detected in LAB. Multiple LAB strains without tetracycline resistance genes were found to be resistant to tetracycline at the currently recommended cutoff values. Thus, based on the minimum inhibitory concentrations of tetracycline for these LAB strains, the species-specific microbiological cutoff values for Lactobacillus (para)gasseri, Lactobacillus johnsonii, and Lactobacillus crispatus to tetracycline were first developed using the Turnidge, Kronvall, and eyeball methods. The cutoff values for Lactiplantibacillus plantarum were re-established and could be used to better distinguish susceptible strains from strains with acquired resistance. Finally, we verified that these five genes play a role in tetracycline resistance and found that tet(M) and tet(W/N/W) are the most widely distributed tetracycline resistance genes in LAB.
Soil Layers Matter: Vertical Stratification of Root-Associated Fungal Assemblages in Temperate Forests Reveals Differences in Habitat Colonization
Authors: Khokon Anis Mahmud , Schneider Dominik , Daniel Rolf , Polle Andrea
Published: October 2021

Ectomycorrhizal and saprotrophic fungi play pivotal roles in ecosystem functioning. Here, we studied the vertical differentiation of root-associated fungi (RAF) in temperate forests. We analysed RAF assemblages in the organic and mineral soil from 150 experimental forest plots across three biogeographic regions spanning a distance of about 800 km. Saprotrophic RAF showed the highest richness in organic and symbiotrophic RAF in mineral soil. Symbiotrophic RAF exhibited higher relative abundances than saprotrophic fungi in both soil layers. Beta-diversity of RAF was mainly due to turnover between organic and mineral soil and showed regional differences for symbiotrophic and saprotrophic fungi. Regional differences were also found for different phylogenetic levels, i.e., fungal orders and indicator species in the organic and mineral soil, supporting that habitat conditions strongly influence differentiation of RAF assemblages. Important exceptions were fungal orders that occurred irrespective of the habitat conditions in distinct soil layers across the biogeographic gradient: Russulales and Cantharellales (ectomycorrhizal fungi) were enriched in RAF assemblages in mineral soil, whereas saprotrophic Polyporales and Sordariales and ectomycorrhizal Boletales were enriched in RAF assemblages in the organic layer. These results underpin a phylogenetic signature for niche partitioning at the rank of fungal orders and suggest that RAF assembly entails two strategies encompassing flexible and territorial habitat colonization by different fungal taxa.
Mineralogical Evolution of the Cretaceous Strata in the Songliao Basin, Northeastern China: Implications for Thermal History and Paleoenvironmental Evolution
Authors: Dong Tian , Gao Yuan , Huang He , Tian Xing , Yang Qian , Li Yuyin , Niu Lu , Cao Yongqiang
Published: October 2021

The Songliao Basin in northeastern China is one of the largest and longest-lived Cretaceous sedimentary basins enriched in petroleum and geothermal resources worldwide. Although the modern Songliao Basin has a high geothermal gradient, the geological thermal history of the basin has not been well constrained. The SK-2 drilling program, as the second stage of the International Continental Drilling Project of Cretaceous Songliao Basin, is for recovering extensive Early Cretaceous terrestrial strata and providing valuable materials for decoding the mineralogical evolution and the paleoenvironmental changes. Here, we present whole-rock and clay mineralogical analysis on 72 core samples covering 3346–5705 m of the Shahezi Formation in the SK-2 borehole. The whole-rock minerals mainly include clay minerals, quartz, plagioclase, as well as some calcite, K-feldspar, siderite, and pyrite. The clay mineral assemblages include illite, chlorite, and illite–smectite interlayer minerals. Above 4500 m, clay minerals are dominated by illite and illite–smectite interlayers. Below 4500 m, more plagioclase, K-feldspar, and calcite are present, while illite–smectite interlayers are completely replaced by illite. The whole-rock and clay mineralogical evolution of the Shahezi Formation is primarily controlled by thermal diagenesis, although paleoenvironmental change may act as a minor contribution. Combined with published data from the Upper Cretaceous in SK-1 cores, we infer that Cretaceous greenhouse climatic and environmental changes left fingerprints on whole-rock and clay mineralogical assemblages and that the Songliao Basin reached a maximum burial depth and a peak of thermal evolution at the end of the Cretaceous.
One-Dimensional Convolutional Auto-Encoder for Predicting Furnace Blowback Events from Multivariate Time Series Process Data—A Case Study
Authors: Theunissen Carl Daniel , Bradshaw Steven Martin , Auret Lidia , Louw Tobias Muller
Published: October 2021

Modern industrial mining and mineral processing applications are characterized by large volumes of historical process data. Hazardous events occurring in these processes compromise process safety and therefore overall viability. These events are recorded in historical data and are often preceded by characteristic patterns. Reconstruction-based data-driven models are trained to reconstruct the characteristic patterns of hazardous event-preceding process data with minimal residuals, facilitating effective event prediction based on reconstruction residuals. This investigation evaluated one-dimensional convolutional auto-encoders as reconstruction-based data-driven models for predicting positive pressure events in industrial furnaces. A simple furnace model was used to generate dynamic multivariate process data with simulated positive pressure events to use as a case study. A one-dimensional convolutional auto-encoder was trained as a reconstruction-based model to recognize the data preceding the hazardous events, and its performance was evaluated by comparing it to a fully-connected auto-encoder as well as a principal component analysis reconstruction model. This investigation found that one-dimensional convolutional auto-encoders recognized event-preceding patterns with lower detection delays, higher specificities, and lower missed alarm rates, suggesting that the one-dimensional convolutional auto-encoder layout is superior to the fully connected auto-encoder layout for use as a reconstruction-based event prediction model. This investigation also found that the nonlinear auto-encoder models outperformed the linear principal component model investigated. While the one-dimensional auto-encoder was evaluated comparatively on a simulated furnace case study, the methodology used in this evaluation can be applied to industrial furnaces and other mineral processing applications. Further investigation using industrial data will allow for a view of the convolutional auto-encoder’s absolute performance as a reconstruction-based hazardous event prediction model.
Awn Stem-Derived High-Activity Free-Metal Porous Carbon for Oxidation Reduction
Authors: Zhao Qingyun , Wen Shikai , Hou Junhua
Published: October 2021

Designing oxygen reduction reaction (ORR) catalysts with excellent performance has far-reaching significance. In this work, a high-activity biomass free-metal carbon catalyst with N and S co-doped was successfully prepared by using the KOH activated awn stem powder as the precursor with organic matter pore-forming doping technology, which is named TAAS. The content of pyridine nitrogen groups accounts for up to 36% of the total nitrogen content, and a rich pore structure is formed on the surface and inside, which are considered as the potential active centers of ORR. The results show that the specific surface area of TAAS reaches 191.04 m2/g, which effectively increases the active sites of the catalyst, and the initial potential and half slope potential are as high as 0.90 and 0.76 V vs. RHE, respectively. This study provides a low-cost, environmentally friendly and feasible strategy for the conversion of low-value agricultural and forestry wastes into high value-added products to promote sustainable development of energy and the environment.
Cissus subtetragona Planch. Ameliorates Inflammatory Responses in LPS-induced Macrophages, HCl/EtOH-induced Gastritis, and LPS-induced Lung Injury via Attenuation of Src and TAK1
Authors: Rahmawati Laily , Aziz Nur , Oh Jieun , Hong Yo Han , Woo Byoung Young , Hong Yong Deog , Manilack Philaxay , Souladeth Phetlasy , Jung Ji Hwa , Lee Woo Shin , Jeon Mi Jeong , Kim Taewoo , Hossain Mohammad Amjad , Yum Jinwhoa , Kim Jong-Hoon , Cho Jae Youl
Published: October 2021

Several Cissus species have been used and reported to possess medicinal benefits. However, the anti-inflammatory mechanisms of Cissus subtetragona have not been described. In this study, we examined the potential anti-inflammatory effects of C. subtetragona ethanol extract (Cs-EE) in vitro and in vivo, and investigated its molecular mechanism as well as its flavonoid content. Lipopolysaccharide (LPS)-induced macrophage-like RAW264.7 cells and primary macrophages as well as LPS-induced acute lung injury (ALI) and HCl/EtOH-induced acute gastritis mouse models were utilized. Luciferase assays, immunoblotting analyses, overexpression strategies, and cellular thermal shift assay (CETSA) were performed to identify the molecular mechanisms and targets of Cs-EE. Cs-EE concentration-dependently reduced the secretion of NO and PGE2, inhibited the expression of inflammation-related cytokines in LPS-induced RAW264.7 cells, and decreased NF-κB- and AP-1-luciferase activity. Subsequently, we determined that Cs-EE decreased the phosphorylation events of NF-κB and AP-1 pathways. Cs-EE treatment also significantly ameliorated the inflammatory symptoms of HCl/EtOH-induced acute gastritis and LPS-induced ALI mouse models. Overexpression of HA-Src and HA-TAK1 along with CETSA experiments validated that inhibited inflammatory responses are the outcome of attenuation of Src and TAK1 activation. Taken together, these findings suggest that Cs-EE could be utilized as an anti-inflammatory remedy especially targeting against gastritis and acute lung injury by attenuating the activities of Src and TAK1.
Probiotics Regulate Gut Microbiota: An Effective Method to Improve Immunity
Authors: Wang Xinzhou , Zhang Peng , Zhang Xin
Published: October 2021

Probiotics are beneficial active microorganisms that colonize the human intestines and change the composition of the flora in particular parts of the host. Recently, the use of probiotics to regulate intestinal flora to improve host immunity has received widespread attention. Recent evidence has shown that probiotics play significant roles in gut microbiota composition, which can inhibit the colonization of pathogenic bacteria in the intestine, help the host build a healthy intestinal mucosa protective layer, and enhance the host immune system. Based on the close relationship between the gut microbiota and human immunity, it has become an extremely effective way to improve human immunity by regulating the gut microbiome with probiotics. In this review, we discussed the influence of probiotics on the gut microbiota and human immunity, and the relationship between immunity, probiotics, gut microbiota, and life quality. We further emphasized the regulation of gut microflora through probiotics, thereby enhancing human immunity and improving people’s lives.
The Antimicrobial Activities of Silver Nanoparticles from Aqueous Extract of Grape Seeds against Pathogenic Bacteria and Fungi
Authors: Al-Otibi Fatimah , Alkhudhair Shahad K. , Alharbi Raedah I. , Al-Askar Abdulaziz A. , Aljowaie Reem M. , Al-Shehri Sameha
Published: October 2021

Grape seed extract (GSE) is a natural source of polyphenolic compounds and secondary metabolites, which have been tested for their possible antimicrobial activities. In the current study, we tested the antibacterial and antifungal activities of aqueous GSE and the biosynthesized silver nanoparticles loaded with GSE (GSE-AgNPs) against different pathogens. The biosynthesized GSE-AgNPs were assessed by UV spectroscopy, dynamic light scattering (DLS), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and gas chromatography/mass spectrometry (GC/MS). The antimicrobial activities were assessed against different bacterial and fungal species. DLS analysis showed that GSE-AgNPs had a Z-Average of 91.89 nm while UV spectroscopy showed that GSE-AgNPs had the highest absorbance at a wavelength of ~415 nm. FTIR analysis revealed that both of GSE and GSE-AgNPs consisted of different functional groups, such as hydroxyl, alkenes, alkyne, and aromatic rings. Both FE-SEM and TEM showed that GSE-AgNPs had larger sizes and rough surfaces than GSE and AgNO3. The results showed significant antimicrobial activities of GSE-AgNPs against all tested species, unlike GSE, which had weaker and limited effects. More studies are needed to investigate the other antimicrobial activities of GSE.
Rhytidhylides A and B, Two New Phthalide Derivatives from the Endophytic Fungus Rhytidhysteron sp. BZM-9
Authors: Zhang Sha , Chen Dekun , Kuang Min , Peng Weiwei , Chen Yan , Tan Jianbing , Kang Fenghua , Xu Kangping , Zou Zhenxing
Published: October 2021

Two new phthalide derivatives, rhytidhylides A (1) and B (2), together with ten known compounds (3–12) were isolated from cultures of Rhytidhysteron sp. BZM-9, an endophyte isolated from the leaves of Leptospermum brachyandrum. Their structures were identified by an extensive analysis of NMR, HRESIMS, ECD, and through comparison with data reported in the literature. In addition, the cytotoxic activities against two human hepatoma cell lines (HepG2 and SMMC7721) and antibacterial activities against MRSA and E. coli were evaluated.
Revision and Extension of a Generally Applicable Group-Additivity Method for the Calculation of the Standard Heat of Combustion and Formation of Organic Molecules
Authors: Naef Rudolf , Acree William E.
Published: October 2021

The calculation of the heats of combustion ΔH°c and formation ΔH°f of organic molecules at standard conditions is presented using a commonly applicable computer algorithm based on the group-additivity method. This work is a continuation and extension of an earlier publication. The method rests on the complete breakdown of the molecules into their constituting atoms, these being further characterized by their immediate neighbor atoms. The group contributions are calculated by means of a fast Gauss–Seidel fitting calculus using the experimental data of 5030 molecules from literature. The applicability of this method has been tested by a subsequent ten-fold cross-validation procedure, which confirmed the extraordinary accuracy of the prediction of ΔH°c with a correlation coefficient R2 and a cross-validated correlation coefficient Q2 of 1, a standard deviation σ of 18.12 kJ/mol, a cross-validated standard deviation S of 19.16 kJ/mol, and a mean absolute deviation of 0.4%. The heat of formation ΔH°f has been calculated from ΔH°c using the standard enthalpies of combustion for the elements, yielding a correlation coefficient R2 for ΔH°f of 0.9979 and a corresponding standard deviation σ of 18.14 kJ/mol.
Design, Microwave-Assisted Synthesis and In Silico Prediction Study of Novel Isoxazole Linked Pyranopyrimidinone Conjugates as New Targets for Searching Potential Anti-SARS-CoV-2 Agents
Authors: Algethami Faisal K. , Cherif Maher , Jlizi Salma , Ben Hamadi Naoufel , Romdhane Anis , Elamin Mohamed R. , Alghamdi Mashael A. , Ben Jannet Hichem
Published: October 2021

A series of novel naphthopyrano[2,3-d]pyrimidin-11(12H)-one containing isoxazole nucleus 4 was synthesized under microwave irradiation and classical conditions in moderate to excellent yields upon 1,3-dipolar cycloaddition reaction using various arylnitrile oxides under copper(I) catalyst. A one-pot, three-component reaction, N-propargylation and Dimroth rearrangement were used as the key steps for the preparation of the dipolarophiles3. The structures of the synthesized compounds were established by 1H NMR, 13C NMR and HRMS-ES means. The present study aims to also predict the theoretical assembly of the COVID-19 protease (SARS-CoV-2 Mpro) and to discover in advance whether this protein can be targeted by the compounds 4a–1 and thus be synthesized. The docking scores of these compounds were compared to those of the co-crystallized native ligand inhibitor (N3) which was used as a reference standard. The results showed that all the synthesized compounds (4a–l) gave interesting binding scores compared to those of N3 inhibitor. It was found that compounds 4a, 4e and 4i achieved greatly similar binding scores and modes of interaction than N3, indicating promising affinity towards SARS-CoV-2 Mpro. On the other hand, the derivatives 4k, 4h and 4j showed binding energy scores (−8.9, −8.5 and −8.4 kcal/mol, respectively) higher than the Mpro N3 inhibitor (−7.0 kcal/mol), revealing, in their turn, a strong interaction with the target protease, although their interactions were not entirely comparable to that of the reference N3.
The Effect of Wall Material Type on the Encapsulation Efficiency and Oxidative Stability of Fish Oils
Authors: Selim Khaled A. , Alharthi Salman S. , Abu El-Hassan Abdelmonam M. , Elneairy Nady A. , Rabee Laila A. , Abdel-Razek Adel G.
Published: October 2021

Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits. It also contains vitamins that are lipid-soluble, such as vitamins A and D. This work aimed to determine how the wall material composition influenced the encapsulation efficiency and oxidative stability of omega fish oils in spray-dried microcapsules. In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying. The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated. All three encapsulating agents provided a highly protective effect against the oxidative deterioration of the encapsulated oils. Whey protein was found to be the most effective encapsulated agent comparing to gum Arabic and maltodextrin. The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively. Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period. It could be concluded that all the encapsulating agents provided a protective effect to the encapsulated fish oil and elongated the shelf life of it comparing to the untreated oil sample (control). The results suggest that encapsulation of fish oil is beneficial for its oxidative stability and its uses in the production of functional foods.
Advances in Skin Wound and Scar Repair by Polymer Scaffolds
Authors: Zhou Shuiqing , Wang Qiusheng , Huang Ao , Fan Hongdou , Yan Shuqin , Zhang Qiang
Published: October 2021

Scars, as the result of abnormal wound-healing response after skin injury, may lead to loss of aesthetics and physical dysfunction. Current clinical strategies, such as surgical excision, laser treatment, and drug application, provide late remedies for scarring, yet it is difficult to eliminate scars. In this review, the functions, roles of multiple polymer scaffolds in wound healing and scar inhibition are explored. Polysaccharide and protein scaffolds, an analog of extracellular matrix, act as templates for cell adhesion and migration, differentiation to facilitate wound reconstruction and limit scarring. Stem cell-seeded scaffolds and growth factors-loaded scaffolds offer significant bioactive substances to improve the wound healing process. Special emphasis is placed on scaffolds that continuously release oxygen, which greatly accelerates the vascularization process and ensures graft survival, providing convincing theoretical support and great promise for scarless healing.
Effect of Biologically Oriented Preparation Technique on the Stress Concentration of Endodontically Treated Upper Central Incisor Restored with Zirconia Crown: 3D-FEA
Authors: Sichi Luigi Giovanni Bernardo , Pierre Fernanda Zapater , Arcila Laura Viviana Calvache , de Andrade Guilherme Schmitt , Tribst João Paulo Mendes , Ausiello Pietro , di Lauro Alessandro Espedito , Borges Alexandre Luiz Souto
Published: October 2021

The aim of this study was to evaluate the effect of biologically oriented preparation technique on the stress concentration of endodontically treated upper central incisors restored with zirconia crown (yttria-stabilized zirconia polycrystalline ceramic) through finite element analysis (FEA). Four models of maxillary central incisors containing enamel, dentin, periodontal ligament, cortical and medullary bone were created in CAD. Each model received a polymeric core-build up with nanofilled dental resin composite. The evaluated models were SM—preparation in shoulder 90°; CM—chamfer preparation; BOPT—biologically oriented preparation technique and BOPTB—BOPT preparation 1 mm below the cement-enamel junction. All models received zirconia crowns (5Y-TZP), fiberglass post and 1 mm ferrule. The models were imported into the analysis software with parameters for mechanical structural testing using the maximum principal stress and the tensile strength as the analysis criteria. Then, load of 150 N was applied at the cingulum with 45° slope to the long axis of the tooth, with the fixed base for each model. The type of marginal preparation affected the stresses concentration in endodontically treated teeth and in the zirconia crown margin. Considering the stress magnitude only, BOPT is a viable option for anterior monolithic zirconia crowns; however, with the highest stress magnitude at the restoration margin.
Natural Aporphine Alkaloids with Potential to Impact Metabolic Syndrome
Authors: Wang Fei-Xuan , Zhu Nan , Zhou Fan , Lin Dong-Xiang
Published: October 2021

The incidence and prevalence of metabolic syndrome has steadily increased worldwide. As a major risk factor for various diseases, metabolic syndrome has come into focus in recent years. Some natural aporphine alkaloids are very promising agents in the prevention and treatment of metabolic syndrome and its components because of their wide variety of biological activities. These natural aporphine alkaloids have protective effects on the different risk factors characterizing metabolic syndrome. In this review, we highlight the activities of bioactive aporphine alkaloids: thaliporphine, boldine, nuciferine, pronuciferine, roemerine, dicentrine, magnoflorine, anonaine, apomorphine, glaucine, predicentrine, isolaureline, xylopine, methylbulbocapnine, and crebanine. We particularly focused on their impact on metabolic syndrome and its components, including insulin resistance and type 2 diabetes mellitus, endothelial dysfunction, hypertension and cardiovascular disease, hyperlipidemia and obesity, non-alcoholic fatty liver disease, hyperuricemia and kidney damage, erectile dysfunction, central nervous system-related disorder, and intestinal microbiota dysbiosis. We also discussed the potential mechanisms of actions by aporphine alkaloids in metabolic syndrome.
Novel Bio-Based Amphiphilic Ionic Liquids for the Efficient Demulsification of Heavy Crude Oil Emulsions
Authors: Abdullah Mahmood M. S. , Al-Lohedan Hamad A.
Published: October 2021

In the last few decades, there has been an increasing trend for the usage of natural products and their derivatives as green and renewable oil-filed chemicals. Use of these compounds or their derivatives contributes to reducing the use of traditional chemicals, and enhances green chemistry principles. Curcumin (CRC) is one of the most popular natural products and is widely available. The green character, antioxidant action, and low cost of CRC prompt its use in several applications. In the present study, Curcumin was used to synthesize two new amphiphilic ionic liquids (AILs) by reacting with 1,3-propanesultone or bromoacetic acid to produce corresponding sulfonic and carboxylic acids, CRC-PS and CRC-BA, respectively. Following this, the formed CRC-PS and CRC-BA were allowed to react with 12-(2-hydroxyethyl)-15-(4-nonylphenoxy)-3,6,9-trioxa-12-azapentadecane-1,14-diol (HNTA) to form corresponding AILs, GCP-IL and GRB-IL, respectively. The chemical structures, surface tension, interfacial tension, and relative solubility number (RSN) of the synthesized AILs were investigated. The efficiency of GCP-IL and GRB-IL to demulsify water in heavy crude oil (W/O) emulsions was also investigated, where we observed that both GCP-IL and GRB-IL served as high-efficiency demulsifiers and the efficiency increased with a decreased ratio of water in W/O emulsion. Moreover, the data showed an increased efficiency of these AILs with an increased concentration. Among the two AILs, under testing conditions, GCP-IL exhibited a higher efficiency, shorter demulsification time, and cleaner demulsified water.
Critical Protein–Protein Interactions Determine the Biological Activity of Elk-1, a Master Regulator of Stimulus-Induced Gene Transcription
Authors: Thiel Gerald , Backes Tobias M. , Guethlein Lisbeth A. , Rössler Oliver G.
Published: October 2021

Elk-1 is a transcription factor that binds together with a dimer of the serum response factor (SRF) to the serum-response element (SRE), a genetic element that connects cellular stimulation with gene transcription. Elk-1 plays an important role in the regulation of cellular proliferation and apoptosis, thymocyte development, glucose homeostasis and brain function. The biological function of Elk-1 relies essentially on the interaction with other proteins. Elk-1 binds to SRF and generates a functional ternary complex that is required to activate SRE-mediated gene transcription. Elk-1 is kept in an inactive state under basal conditions via binding of a SUMO-histone deacetylase complex. Phosphorylation by extracellular signal-regulated protein kinase, c-Jun N-terminal protein kinase or p38 upregulates the transcriptional activity of Elk-1, mediated by binding to the mediator of RNA polymerase II transcription (Mediator) and the transcriptional coactivator p300. Strong and extended phosphorylation of Elk-1 attenuates Mediator and p300 recruitment and allows the binding of the mSin3A-histone deacetylase corepressor complex. The subsequent dephosphorylation of Elk-1, catalyzed by the protein phosphatase calcineurin, facilitates the re-SUMOylation of Elk-1, transforming Elk-1 back to a transcriptionally inactive state. Thus, numerous protein–protein interactions control the activation cycle of Elk-1 and are essential for its biological function.
Theoretical Study on Redox Potential Control of Iron-Sulfur Cluster by Hydrogen Bonds: A Possibility of Redox Potential Programming
Authors: Era Iori , Kitagawa Yasutaka , Yasuda Natsumi , Kamimura Taigo , Amamizu Naoka , Sato Hiromasa , Cho Keigo , Okumura Mitsutaka , Nakano Masayoshi
Published: October 2021

The effect of hydrogen bonds around the active site of Anabaena [2Fe-2S] ferredoxin (Fd) on a vertical ionization potential of the reduced state (IP(red)) is examined based on the density functional theory (DFT) calculations. The results indicate that a single hydrogen bond increases the relative stability of the reduced state, and shifts IP(red) to a reductive side by 0.31–0.33 eV, regardless of the attached sulfur atoms. In addition, the IP(red) value can be changed by the number of hydrogen bonds around the active site. The results also suggest that the redox potential of [2Fe-2S] Fd is controlled by the number of hydrogen bonds because IP(red) is considered to be a major factor in the redox potential. Furthermore, there is a possibility that the redox potentials of artificial iron-sulfur clusters can be finely controlled by the number of the hydrogen bonds attached to the sulfur atoms of the cluster.
Heart Rate Sharing at the Workplace
Authors: Wikström Valtteri , Falcon Mari , Martikainen Silja , Pejoska Jana , Durall Eva , Bauters Merja , Saarikivi Katri
Published: October 2021

Augmenting online interpersonal communication with biosignals, often in the form of heart rate sharing, has shown promise in increasing affiliation, feelings of closeness, and intimacy. Increasing empathetic awareness in the professional domain and in the customer interface could benefit both customer and employee satisfaction, but heart rate sharing in this context needs to consider issues around physiological monitoring of employees, appropriate level of intimacy, as well as the productivity outlook. In this study, we explore heart rate sharing at the workplace and study its effects on task performance. Altogether, 124 participants completed a collaborative visual guidance task using a chat box with heart rate visualization. Participants’ feedback about heart rate sharing reveal themes such as a stronger sense of human contact and increased self-reflection, but also raise concerns around unnecessity, intimacy, privacy and negative interpretations. Live heart rate was always measured, but to investigate the effect of heart rate sharing on task performance, half of the customers were told that they were seeing a recording, and half were told that they were seeing the advisor’s live heart beat. We found a negative link between awareness and task performance. We also found that higher ratings of usefulness of the heart rate visualization were associated with increased feelings of closeness. These results reveal that intimacy and privacy issues are particularly important for heart rate sharing in professional contexts, that preference modulates the effects of heart rate sharing on social closeness, and that heart rate sharing may have a negative effect on performance.