bluuebunny/crossref_metadata_2025 huggingface dataset:
The Interannual Variability of Global Burned Area Is Mostly Explained by Climatic Drivers
Authors: Gincheva Andrina , Pausas Juli G. , Torres‐Vázquez Miguel Ángel , Bedia Joaquín , Vicente‐Serrano Sergio M. , Abatzoglou John T. , Sánchez‐Espigares Josep A. , Chuvieco Emilio , Jerez Sonia , Provenzale Antonello , Trigo Ricardo M. , Turco Marco
Published: July 2024

Better understanding how fires respond to climate variability is an issue of current interest in light of ongoing climate change. However, evaluating the global‐scale temporal variability of fires in response to climate presents a challenge due to the intricate processes at play and the limitation of fire data. Here, we investigate the links between year‐to‐year variability of burned area (BA) and climate using BA data, the Fire Weather Index (FWI), and the Standardized Precipitation Evapotranspiration Index (SPEI) from 2001 to 2021 at ecoregion scales. Our results reveal complex spatial patterns in the dependence of BA variability on antecedent and concurrent weather conditions, highlighting where BA is mostly influenced by either FWI or SPEI and where the combined effect of both indicators must be considered. Our findings indicate that same‐season weather conditions have a more pronounced relationship with BA across various ecoregions, particularly in climatologically wetter areas. Additionally, we note that BA is also significantly associated with periods of antecedent wetness and coolness, with this association being especially evident in more arid ecoregions. About 60% of the interannual variations in BA can be explained by climatic variability in a large fraction (∼77%) of the world's burnable regions.
Paleozoic Episodic Magmatism in Western Tianshan: Insight Into Assembling the Northeastern Pangea
Authors: Wang Kai , Cai Keda , Sun Min , Lai Chun‐Kit , Xia Xiao‐Ping , Wang Xiangsong , Bao Zihe
Published: July 2024

The late‐stage union of Pangea was associated with the convergence of Siberia with Laurussia, but the exact timing remains unclear. The orogenic duration of the Kazakhstan block can provide geochronological constraints as it connects Siberia, Baltica, and Tarim. Zircon petrochronology offers a reliable approach for ascertaining the lifespan of an ancient orogen. In this study, we explore three phases of magmatism recorded in detrital zircons from late Paleozoic and Mesozoic sandstone‐siltstones in Western Tianshan, that is, 400–470 Ma, 320–380 Ma, and 280–320 Ma. Based on their age‐propagated Hf isotopes, melt SiO2 contents, and crustal thicknesses, our findings suggest that the southern limb of Kazakhstan underwent the early Paleozoic amalgamation of microcontinents with arcs, the late Paleozoic maturation of an Andean‐like continental arc, and the late Carboniferous collision of Kazakhstan with the Junggar oceanic basin and the Tarim craton. Such characteristics manifest the long‐term orogenic progression of Kazakhstan. Combining published timelines and paleolatitudes of major orogens and blocks, we propose that the Kazakhstan block welded northeastern Pangea along with the cessation of these orogenic activities around it. Consequently, by docking Kazakhstan with surrounding cratons, the fundamental configuration of Pangea could have been established in the late Carboniferous.
Convective Initiation Nowcasting in South China Using Physics‐Augmented Random Forest Models and Geostationary Satellites
Authors: Yang Chunlei , Yuan Huiling , Zhang Feng , Xie Meng , Wang Yan , Jiang Geng‐Ming
Published: July 2024

Convective initiation (CI) nowcasting in subtropical regions often faces challenges, such as complex physical processes and imbalanced samples of CI events, resulting in a high false alarm ratio (FAR). In this paper, we propose a Storm Warning System with Physics‐Augmentation (SWASP) based on the random forest algorithm and cloud physical conditions, using Himawari‐8 Advanced Himawari Imager data from April to September 2019 in South China. The cloud physical conditions (e.g., cloud‐top cooling rates) were investigated to establish regional thresholds for convection occurrence. Ancillary information, including elevation, satellite zenith angle, and latitude, was also incorporated into the SWASP model. Compared to conventional methods, the SWASP model exhibits an improved probability of detection by 0.11 and 0.08 and a decreased FAR by 0.38 and 0.44 for daytime and nighttime forecasts. Moreover, the SWASP model enables the detection of local convective storm systems about 30 min to 1 hr ahead of radar detection in typical convective storm cases. This study contributes to further advancements of the SWASP model by incorporating physical conditions and emphasizes the potential application of geostationary satellites in convective early warnings.
Investigation of Dust‐Induced Direct Radiative Forcing Over the Arabian Peninsula Based on High‐Resolution WRF‐Chem Simulations
Authors: Karumuri Rama Krishna , Dasari Hari Prasad , Gandham Harikishan , Kunchala Ravi Kumar , Attada Raju , Ashok Karumuri , Hoteit Ibrahim
Published: July 2024

This study investigates the impact of dust on radiation over the Arabian Peninsula (AP) during the reported high, low, and normal dust seasons (March–August) of 2012, 2014, and 2015, respectively. Simulations were performed using the Weather Research and Forecasting model coupled to a Chemistry module (WRF‐Chem). The simulated seasonal horizontal and vertical dust concentrations, and their interannual distinctions, match well with those from two ground‐based AERONET observations, and measurements from MODIS and CALIOP satellites. The maximum dust concentrations over the dust‐source regions in the southern AP reach vertically upto 700 hPa during the high dust season, but only upto 900–950 hPa during the low/normal dust seasons. Stronger incoming low‐level winds along the southern Red Sea and those from Iraq bring in higher‐than‐normal dust during the high dust summers. We conducted a sensitivity experiment by switching‐off the dust module to assess the radiative perturbations due to dust. The results suggest that active dust‐module improved the fidelity of simulated radiation fluxes distributions at the surface and top of the atmosphere vis‐à‐vis Clouds and the Earth's Radiant Energy System (CERES) measurements. Dust results in a 26 Wm−2 short‐wave (SW) radiative forcing in the tropospheric‐column over the AP. The SW radiative forcing increases by another 6–8 Wm−2 during the high dust season due to the increased number of extreme dust days, which also amplifies atmospheric heating. During extreme dust days, the heating rate exhibits a dipolar structure, with cooling over the Iraq region and warming of 40%–60% over the southern‐AP.
Network properties determine neural network performance
Authors: Jiang Chunheng , Huang Zhenhan , Pedapati Tejaswini , Chen Pin-Yu , Sun Yizhou , Gao Jianxi
Published: July 2024

Machine learning influences numerous aspects of modern society, empowers new technologies, from Alphago to ChatGPT, and increasingly materializes in consumer products such as smartphones and self-driving cars. Despite the vital role and broad applications of artificial neural networks, we lack systematic approaches, such as network science, to understand their underlying mechanism. The difficulty is rooted in many possible model configurations, each with different hyper-parameters and weighted architectures determined by noisy data. We bridge the gap by developing a mathematical framework that maps the neural network’s performance to the network characters of the line graph governed by the edge dynamics of stochastic gradient descent differential equations. This framework enables us to derive a neural capacitance metric to universally capture a model’s generalization capability on a downstream task and predict model performance using only early training results. The numerical results on 17 pre-trained ImageNet models across five benchmark datasets and one NAS benchmark indicate that our neural capacitance metric is a powerful indicator for model selection based only on early training results and is more efficient than state-of-the-art methods.
Heterogeneity in lung macrophage control of Mycobacterium tuberculosis is modulated by T cells
Authors: Lai Rocky , Williams Travis , Rakib Tasfia , Lee Jinhee , Behar Samuel M.
Published: July 2024

Following Mycobacterium tuberculosis infection, alveolar macrophages are initially infected but ineffectively restrict bacterial replication. The distribution of M. tuberculosis among different cell types in the lung changes with the onset of T cell immunity when the dominant infected cellular niche shifts from alveolar to monocyte-derived macrophages (MDM). We hypothesize that changes in bacterial distribution among different cell types is driven by differences in T cell recognition of infected cells and their subsequent activation of antimicrobial effector mechanisms. We show that CD4 and CD8 T cells efficiently eliminate M. tuberculosis infection in alveolar macrophages, but they have less impact on suppressing infection in MDM, which may be a bacterial niche. Importantly, CD4 T cell responses enhance MDM recruitment to the lung. Thus, the outcome of infection depends on the interaction between the T cell subset and the infected cell; both contribute to the resolution and persistence of the infection.
Ocean iron cycle feedbacks decouple atmospheric CO2 from meridional overturning circulation changes
Authors: Lauderdale Jonathan Maitland
Published: July 2024

The ocean’s Meridional Overturning Circulation (MOC) brings carbon- and nutrient-rich deep waters to the surface around Antarctica. Limited by light and dissolved iron, photosynthetic microbes incompletely consume these nutrients, the extent of which governs the escape of inorganic carbon into the atmosphere. Changes in MOC upwelling may have regulated Southern Ocean outgassing, resulting in glacial-interglacial atmospheric CO2 oscillations. However, numerical models that explore this positive relationship do not typically include a feedback between biological activity and abundance of organic chelating ligands that control dissolved iron availability. Here, I show that incorporating a dynamic ligand parameterization inverts the modelled MOC-atmospheric CO2 relationship: reduced MOC nutrient upwelling decreases biological activity, resulting in scant ligand production, enhanced iron limitation, incomplete nutrient usage, and ocean carbon outgassing, and vice versa. This first-order response suggests iron cycle feedbacks may be a critical driver of the ocean’s response to climate changes, independent of external iron supply.
Ube3a unsilencer for the potential treatment of Angelman syndrome
Authors: Vihma Hanna , Li Kelin , Welton-Arndt Anna , Smith Audrey L. , Bettadapur Kiran R. , Gilmore Rachel B. , Gao Eric , Cotney Justin L. , Huang Hsueh-Cheng , Collins Jon L. , Chamberlain Stormy J. , Lee Hyeong-Min , Aubé Jeffrey , Philpot Benjamin D.
Published: July 2024

Deletion of the maternal UBE3A allele causes Angelman syndrome (AS); because paternal UBE3A is epigenetically silenced by a long non-coding antisense (UBE3A-ATS) in neurons, this nearly eliminates UBE3A protein in the brain. Reactivating paternal UBE3A holds promise for treating AS. We previously showed topoisomerase inhibitors can reactivate paternal UBE3A, but their therapeutic challenges prompted our search for small molecule unsilencers with a different mechanism of action. Here, we found that (S)-PHA533533 acts through a novel mechanism to significantly increase paternal Ube3a mRNA and UBE3A protein levels while downregulating Ube3a-ATS in primary neurons derived from AS model mice. Furthermore, peripheral delivery of (S)-PHA533533 in AS model mice induces widespread neuronal UBE3A expression. Finally, we show that (S)-PHA533533 unsilences paternal UBE3A in AS patient-derived neurons, highlighting its translational potential. Our findings provide a lead for developing a small molecule treatment for AS that could be safe, non-invasively delivered, and capable of brain-wide unsilencing of paternal UBE3A.
Copy number variation introduced by a massive mobile element facilitates global thermal adaptation in a fungal wheat pathogen
Authors: Tralamazza Sabina Moser , Gluck-Thaler Emile , Feurtey Alice , Croll Daniel
Published: July 2024

Copy number variation (CNV) can drive rapid evolution in changing environments. In microbial pathogens, such adaptation is a key factor underpinning epidemics and colonization of new niches. However, the genomic determinants of such adaptation remain poorly understood. Here, we systematically investigate CNVs in a large genome sequencing dataset spanning a worldwide collection of 1104 genomes from the major wheat pathogen Zymoseptoria tritici. We found overall strong purifying selection acting on most CNVs. Genomic defense mechanisms likely accelerated gene loss over episodes of continental colonization. Local adaptation along climatic gradients was likely facilitated by CNVs affecting secondary metabolite production and gene loss in general. One of the strongest loci for climatic adaptation is a highly conserved gene of the NAD-dependent Sirtuin family. The Sirtuin CNV locus localizes to an ~68-kb Starship mobile element unique to the species carrying genes highly expressed during plant infection. The element has likely lost the ability to transpose, demonstrating how the ongoing domestication of cargo-carrying selfish elements can contribute to selectable variation within populations. Our work highlights how standing variation in gene copy numbers at the global scale can be a major factor driving climatic and metabolic adaptation in microbial species.
An experimentally validated approach to automated biological evidence generation in drug discovery using knowledge graphs
Authors: Sudhahar Saatviga , Ozer Bugra , Chang Jiakang , Chadwick Wayne , O’Donovan Daniel , Campbell Aoife , Tulip Emma , Thompson Neil , Roberts Ian
Published: July 2024

Explaining predictions for drug repositioning with biological knowledge graphs is a challenging problem. Graph completion methods using symbolic reasoning predict drug treatments and associated rules to generate evidence representing the therapeutic basis of the drug. Yet the vast amounts of generated paths that are biologically irrelevant or not mechanistically meaningful within the context of disease biology can limit utility. We use a reinforcement learning based knowledge graph completion model combined with an automatic filtering approach that produces the most relevant rules and biological paths explaining the predicted drug’s therapeutic connection to the disease. In this work we validate the approach against preclinical experimental data for Fragile X syndrome demonstrating strong correlation between automatically extracted paths and experimentally derived transcriptional changes of selected genes and pathways of drug predictions Sulindac and Ibudilast. Additionally, we show it reduces the number of generated paths in two case studies, 85% for Cystic fibrosis and 95% for Parkinson’s disease.
Egg multivesicular bodies elicit an LC3-associated phagocytosis-like pathway to degrade paternal mitochondria after fertilization
Authors: Ben-Hur Sharon , Sernik Shoshana , Afar Sara , Kolpakova Alina , Politi Yoav , Gal Liron , Florentin Anat , Golani Ofra , Sivan Ehud , Dezorella Nili , Morgenstern David , Pietrokovski Shmuel , Schejter Eyal , Yacobi-Sharon Keren , Arama Eli
Published: July 2024

Mitochondria are maternally inherited, but the mechanisms underlying paternal mitochondrial elimination after fertilization are far less clear. Using Drosophila, we show that special egg-derived multivesicular body vesicles promote paternal mitochondrial elimination by activating an LC3-associated phagocytosis-like pathway, a cellular defense pathway commonly employed against invading microbes. Upon fertilization, these egg-derived vesicles form extended vesicular sheaths around the sperm flagellum, promoting degradation of the sperm mitochondrial derivative and plasma membrane. LC3-associated phagocytosis cascade of events, including recruitment of a Rubicon-based class III PI(3)K complex to the flagellum vesicular sheaths, its activation, and consequent recruitment of Atg8/LC3, are all required for paternal mitochondrial elimination. Finally, lysosomes fuse with strings of large vesicles derived from the flagellum vesicular sheaths and contain degrading fragments of the paternal mitochondrial derivative. Given reports showing that in some mammals, the paternal mitochondria are also decorated with Atg8/LC3 and surrounded by multivesicular bodies upon fertilization, our findings suggest that a similar pathway also mediates paternal mitochondrial elimination in other flagellated sperm-producing organisms.
Network mechanisms of ongoing brain activity’s influence on conscious visual perception
Authors: Wu Yuan-hao , Podvalny Ella , Levinson Max , He Biyu J.
Published: July 2024

Sensory inputs enter a constantly active brain, whose state is always changing from one moment to the next. Currently, little is known about how ongoing, spontaneous brain activity participates in online task processing. We employed 7 Tesla fMRI and a threshold-level visual perception task to probe the effects of prestimulus ongoing brain activity on perceptual decision-making and conscious recognition. Prestimulus activity originating from distributed brain regions, including visual cortices and regions of the default-mode and cingulo-opercular networks, exerted a diverse set of effects on the sensitivity and criterion of conscious recognition, and categorization performance. We further elucidate the mechanisms underlying these behavioral effects, revealing how prestimulus activity modulates multiple aspects of stimulus processing in highly specific and network-dependent manners. These findings reveal heretofore unknown network mechanisms underlying ongoing brain activity’s influence on conscious perception, and may hold implications for understanding the precise roles of spontaneous activity in other brain functions.
Metal-ligand dual-site single-atom nanozyme mimicking urate oxidase with high substrates specificity
Authors: Wang Kaiyuan , Hong Qing , Zhu Caixia , Xu Yuan , Li Wang , Wang Ying , Chen Wenhao , Gu Xiang , Chen Xinghua , Fang Yanfeng , Shen Yanfei , Liu Songqin , Zhang Yuanjian
Published: July 2024

In nature, coenzyme-independent oxidases have evolved in selective catalysis using isolated substrate-binding pockets. Single-atom nanozymes (SAzymes), an emerging type of non-protein artificial enzymes, are promising to simulate enzyme active centers, but owing to the lack of recognition sites, realizing substrate specificity is a formidable task. Here we report a metal-ligand dual-site SAzyme (Ni-DAB) that exhibited selectivity in uric acid (UA) oxidation. Ni-DAB mimics the dual-site catalytic mechanism of urate oxidase, in which the Ni metal center and the C atom in the ligand serve as the specific UA and O2 binding sites, respectively, characterized by synchrotron soft X-ray absorption spectroscopy, in situ near ambient pressure X-ray photoelectron spectroscopy, and isotope labeling. The theoretical calculations reveal the high catalytic specificity is derived from not only the delicate interaction between UA and the Ni center but also the complementary oxygen reduction at the beta C site in the ligand. As a potential application, a Ni-DAB-based biofuel cell using human urine is constructed. This work unlocks an approach of enzyme-like isolated dual sites in boosting the selectivity of non-protein artificial enzymes.
CircCDC42-encoded CDC42-165aa regulates macrophage pyroptosis in Klebsiella pneumoniae infection through Pyrin inflammasome activation
Authors: Xu Nana , Jiang Jiebang , Jiang Fei , Dong Guokai , Meng Li , Wang Meng , Chen Jing , Li Cong , Shi Yongping , He Sisi , Li Rongpeng
Published: July 2024

The circular RNA (circRNA) family is a group of endogenous non-coding RNAs (ncRNAs) that have critical functions in multiple physiological and pathological processes, including inflammation, cancer, and cardiovascular diseases. However, their roles in regulating innate immune responses remain unclear. Here, we define Cell division cycle 42 (CDC42)−165aa, a protein encoded by circRNA circCDC42, which is overexpressed in Klebsiella pneumoniae (KP)-infected alveolar macrophages. High levels of CDC42-165aa induces the hyperactivation of Pyrin inflammasomes and aggravates alveolar macrophage pyroptosis, while the inhibition of CDC42-165aa reduces lung injury in mice after KP infection by inhibiting Pyrin inflammasome-mediated pyroptosis. Overall, these results demonstrate that CDC42-165aa stimulates Pyrin inflammasome by inhibiting CDC42 GTPase activation and provides a potential clinical target for pathogenic bacterial infection in clinical practice.
Donor-acceptor bulk-heterojunction sensitizer for efficient solid-state infrared-to-visible photon up-conversion
Authors: Bi Pengqing , Zhang Tao , Guo Yuanyuan , Wang Jianqiu , Chua Xian Wei , Chen Zhihao , Goh Wei Peng , Jiang Changyun , Chia Elbert E. M. , Hou Jianhui , Yang Le
Published: July 2024

Solid-state infrared-to-visible photon up-conversion is important for spectral-tailoring applications. However, existing up-conversion systems not only suffer from low efficiencies and a need for high excitation intensity, but also exhibit a limited selection of materials and complex fabrication processes. Herein, we propose a sensitizer with a bulk-heterojunction structure, comprising both an energy donor and an energy acceptor, for triplet-triplet annihilation up-conversion devices. The up-conversion occurs through charge separation at the donor-acceptor interface, followed by the formation of charge transfer state between the energy donor and annihilator following the spin statistics. The bulk-heterojunction sensitizer ensures efficient charge generation and low charge recombination. Hence, we achieve a highly efficient solid-state up-conversion device with 2.20% efficiency and low excitation intensity (10 mW cm−2) through a one-step solution method. We also demonstrate bright up-conversion devices on highly-flexible large-area substrates. This study introduces a simple and scalable platform strategy for fabricating efficient up-conversion devices.
Efficient and rapid digestion of proteins with a dual-enzyme microreactor featuring 3-D pores formed by dopamine/polyethyleneimine/acrylamide-coated KIT-6 molecular sieve
Authors: Yuan Fang-Fang , Wang Pei , Han Xiao-Jie , Qin Ting-Ting , Lu Xin , Bai Hai-Jiao
Published: July 2024

The microreactor with two types of immobilized enzymes, exhibiting excellent orthogonal performance, represents an effective approach to counteract the reduced digestion efficiency resulting from the absence of a single enzyme cleavage site, thereby impacting protein identification. In this study, we developed a hydrophilic dual-enzyme microreactor characterized by rapid mass transfer and superior enzymatic activity. Initially, we selected KIT-6 molecular sieve as the carrier for the dual-IMER due to its three-dimensional network pore structure. Modification involved co-deposition of polyethyleneimine (PEI) and acrylamide (AM) as amine donors, along with dopamine to enhance material hydrophilicity. Remaining amino and double bond functional groups facilitated stepwise immobilization of trypsin and Glu-C. Digestion times for bovine serum albumin (BSA) and bovine hemoglobin (BHb) on the dual-IMER were significantly reduced compared to solution-based digestion (1 min vs. 36 h), resulting in improved sequence coverage (91.30% vs. 82.7% for BSA; 90.24% vs. 89.20% for BHb). Additionally, the dual-IMER demonstrated excellent durability, retaining 96.08% relative activity after 29 reuse cycles. Enhanced protein digestion efficiency can be attributed to several factors: (1) KIT-6’s large specific surface area, enabling higher enzyme loading capacity; (2) Its three-dimensional network pore structure, facilitating faster mass transfer and substance diffusion; (3) Orthogonality of trypsin and Glu-C enzyme cleavage sites; (4) The spatial effect introduced by the chain structure of PEI and glutaraldehyde’s spacing arm, reducing spatial hindrance and enhancing enzyme–substrate interactions; (5) Mild and stable enzyme immobilization. The KIT-6-based dual-IMER offers a promising technical tool for protein digestion, while the PDA/PEI/AM-KIT-6 platform holds potential for immobilizing other proteins or active substances.
Dietary nanocomposite of vitamin C and vitamin E enhanced the performance of Nile tilapia
Authors: Sherif Ahmed H. , Khalil Riad H. , Talaat Talaat S. , Baromh Mohamed Z. , Elnagar Mahmoud A.
Published: July 2024

Nowadays, nanomaterials enter high numbers of daily used products and drug manufacture. A nanocomposite of vitamins C (VC) and vitamin E (VE) with chitosan as a vehicle and protector was used in a comparative eight-week feeding study, Nile tilapia weighing 31.2 ± 0.36 g distributed in seven groups and fed (G1) basal diet, (G2) bulk VC, (G3) VC- nanoparticles (NPs), (G4) bulk VE, (G5) VE-NPs, bulk VCE (G6), and (G7) VC plus VE (VCE)-NPs, respectively. The Nile tilapia-fed nanocomposite vitamins had significantly higher growth performance compared to the control; VCE-NPs had the superiority among tested supplementations where total weight gain (63.6 g), daily weight gain (1.13 g), relative growth rate (206.1%) with lower feed conversion rate (1.6) and insignificant feed intake (101.5 g). Overall, the level of liver enzymes was significantly decreased in fish serum after eight-week nanocomposite supplementation, and dietary VCE-NPs caused a significant reduction of serum AST (18.45 IU/L) and ALT (14.77 IU/L) compared to the control 25.5 IU/L and 17.6 IU/L, respectively. Fish fed dietary VCE-NPs, VC-NPs, and VE-NPs had significant enhancement of RBCs 4.2 × 106/μL, 3.8 × 106/μL, and 3.55 × 106/μL; WBCs 46.15 × 103, 42.9 × 103, and 44 × 103/μL, respectively, Also TP was significantly higher 6.38 g/dL in VCE-NPs group compared to the control and the other treatments. Over all, the dietary nanocomposite vitamins boost the innate immunity of the experimental Nile tilapia, the oxidative burst activity (OBA), phagocytic activity (PA), phagocytic index (PI), and serum antibacterial (SAA) were significantly increased compared to those received bulk vitamins and the control. The activity of antioxidant biomarkers in fish serum including glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (TAC), glutathione reductase (GR), and myeloperoxidase (MPO) showed a rise in the serum of Nile tilapia received nano- and bulk-form of VC and VCE compared to the control and both forms of VE. Furthermore, the level of malondialdehyde (MDA), reduced glutathione (GSH), and oxidized glutathione (GSSG) were significantly increased in the fish serum following the trend of antioxidants enzymes. In conclusion, a dietary nanocomposite of vitamin C and vitamin E enhanced Nile tilapia's growth performance and feed utilization. It could also improve health status and immune response. The values of antioxidant biomarkers indicated that the nanocomposite could help the fish body scavenge the generated reactive oxidative species (ROS).
Non-invasive fractional flow reserve estimation in coronary arteries using angiographic images
Authors: Edrisnia Hadis , Sarkhosh Mohammad Hossein , Mohebbi Bahram , Parhizgar Seyed Ehsan , Alimohammadi Mona
Published: July 2024

Coronary artery disease is the leading global cause of mortality and Fractional Flow Reserve (FFR) is widely regarded as the gold standard for assessing coronary artery stenosis severity. However, due to the limitations of invasive FFR measurements, there is a pressing need for a highly accurate virtual FFR calculation framework. Additionally, it’s essential to consider local haemodynamic factors such as time-averaged wall shear stress (TAWSS), which play a critical role in advancement of atherosclerosis. This study introduces an innovative FFR computation method that involves creating five patient-specific geometries from two-dimensional coronary angiography images and conducting numerical simulations using computational fluid dynamics with a three-element Windkessel model boundary condition at the outlet to predict haemodynamic distribution. Furthermore, four distinct boundary condition methodologies are applied to each geometry for comprehensive analysis. Several haemodynamic features, including velocity, pressure, TAWSS, and oscillatory shear index are investigated and compared for each case. Results show that models with average boundary conditions can predict FFR values accurately and observed errors between invasive FFR and virtual FFR are found to be less than 5%.
Acute toxicity of the fungicide captan to honey bees and mixed evidence for synergism with the insecticide thiamethoxam
Authors: De Souza Daiana , Urbanowicz Christine , Ng Wee Hao , Baert Nicolas , Fersch Ashley A. , Smith Michael L. , McArt Scott H.
Published: July 2024

Honey bees are commonly co-exposed to pesticides during crop pollination, including the fungicide captan and neonicotinoid insecticide thiamethoxam. We assessed the impact of exposure to these two pesticides individually and in combination, at a range of field-realistic doses. In laboratory assays, mortality of larvae treated with captan was 80–90% greater than controls, dose-independent, and similar to mortality from the lowest dose of thiamethoxam. There was evidence of synergism (i.e., a non-additive response) from captan-thiamethoxam co-exposure at the highest dose of thiamethoxam, but not at lower doses. In the field, we exposed whole colonies to the lowest doses used in the laboratory. Exposure to captan and thiamethoxam individually and in combination resulted in minimal impacts on population growth or colony mortality, and there was no evidence of synergism or antagonism. These results suggest captan and thiamethoxam are each acutely toxic to immature honey bees, but whole colonies can potentially compensate for detrimental effects, at least at the low doses used in our field trial, or that methodological differences of the field experiment impacted results (e.g., dilution of treatments with natural pollen). If compensation occurred, further work is needed to assess how it occurred, potentially via increased queen egg laying, and whether short-term compensation leads to long-term costs. Further work is also needed for other crop pollinators that lack the social detoxification capabilities of honey bee colonies and may be less resilient to pesticides.
A dynamic attribute reduction algorithm based on relative neighborhood discernibility degree
Authors: Feng Weibing , Sun Tiantian
Published: July 2024

This paper addresses the current existence of attribute reduction algorithms for incomplete hybrid decision-making systems, including low attribute reduction efficiency, low classification accuracy and lack of consideration of unlabeled data types. To address these issues, this paper first redefines the weakly labeled relative neighborhood discernibility degree and develops a non-dynamic attribute reduction algorithm. In addition, this paper proposes an incremental update mechanism for weakly tagged relative neighborhood discernibility degree and introduces a new dynamic attribute reduction algorithm for increasing the set of objects based on it. Meanwhile, this paper also compares and analyses the improved algorithm proposed in this study with two existing attribute reduction algorithms using 8 data sets in the UCI database. The results show that the dynamic attribute reduction algorithm proposed in this paper achieves higher attribute reduction efficiency and classification accuracy, which further validates the effectiveness of the algorithm proposed in this paper.
Enhancing brain tumor segmentation in MRI images using the IC-net algorithm framework
Authors: D S Chandra Sekaran , Christopher Clement J.
Published: July 2024

Brain tumors, often referred to as intracranial tumors, are abnormal tissue masses that arise from rapidly multiplying cells. During medical imaging, it is essential to separate brain tumors from healthy tissue. The goal of this paper is to improve the accuracy of separating tumorous regions from healthy tissues in medical imaging, specifically for brain tumors in MRI images which is difficult in the field of medical image analysis. In our research work, we propose IC-Net (Inverted-C), a novel semantic segmentation architecture that combines elements from various models to provide effective and precise results. The architecture includes Multi-Attention (MA) blocks, Feature Concatenation Networks (FCN), Attention-blocks which performs crucial tasks in improving brain tumor segmentation. MA-block aggregates multi-attention features to adapt to different tumor sizes and shapes. Attention-block is focusing on key regions, resulting in more effective segmentation in complex images. FCN-block captures diverse features, making the model more robust to various characteristics of brain tumor images. Our proposed architecture is used to accelerate the training process and also to address the challenges posed by the diverse nature of brain tumor images, ultimately leads to potentially improved segmentation performance. IC-Net significantly outperforms the typical U-Net architecture and other contemporary effective segmentation techniques. On the BraTS 2020 dataset, our IC-Net design obtained notable outcomes in Accuracy, Loss, Specificity, Sensitivity as 99.65, 0.0159, 99.44, 99.86 and DSC (core, whole, and enhancing tumors as 0.998717, 0.888930, 0.866183) respectively.
In vivo and in vitro efficacy of the ithmid kohl/zinc-oxide nanoparticles, ithmid kohl/Aloe vera, and zinc-oxide nanoparticles/Aloe vera for the treatment of bacterial endophthalmitis
Authors: Obaid Hasson Shaimaa , Kamil Hasan Hasanain , Abdul Kadhem Salman Sumod , Judi Hawraa K. , Akrami Sousan , Saki Morteza , Adil Hasan Maryam , Fares Hashem Durah
Published: July 2024

The aim of this study was to investigate the efficacy of the ithmid kohl/zinc-oxide nanoparticles (ZnONPs), ithmid kohl/Aloe vera, and ZnONPs/Aloe verain the treatment of bacterial endophthalmitis. The endophthalmitis model was prepared by contaminating both eyes of 24 healthy adult male albino rabbits with a clinical isolate ofKlebsiella pneumoniae. The animals were randomly divided into eight groups (A-H) according to the treatment. Group A received 1 ml of ithmid kohl/ZnONPs ointment, group B received 1 ml of ithmid kohl/Aloe veragel ointment, group C received 1 ml of ZnONPs/Aloe veragel ointment, and groups D, E, and F were treated with 1 ml of ithmid kohl solution (0.5 g/ml in distilled water), 1 ml of ZnONPs (0.5 g/ml) colloidal dispersion, and 1 ml ofAloe veragel, respectively. Group G received 100 μl of a tetracycline antibiotic solution (final concentration: 16 µg/ml), and group H received sterile distilled water (no treatment). In vitro antibacterial activity was evaluated againstK. pneumoniaeusing the agar well diffusion. The combination of ithmid kohl/ZnONPs was the most effective formulation for treating endophthalmitis model in infected rabbits within 2 days. In vitro antibacterial assay confirmed the potential of the ithmid kohl/ZnONPs formulation, which had the largest zone of inhibition (31 mm) among the compounds tested. The preparation of the ithmid kohl/ZnONPs formulation and its in vivo experiment in albino rabbits for the treatment of bacterial endophthalmitis was an innovative approach that has shown promise and may potentially serve as a viable alternative in clinical practice.
Variational approach for Stokes flow through a two-dimensional non-uniform channel
Authors: Banerjee Abhishek , Oron Alexander , Agnon Yehuda
Published: July 2024

A variational approach is proposed to study the Stokes flow in a two-dimensional non-uniform channel. By using the stationarity of the Lagrangian, the Euler-Lagrange equations are established which leads to a simple set of ordinary differential equations to provide an estimate for the average pressure drop explicitly in terms of the channel shape function. The results for the pressure drop show an excellent agreement with the second-order extended lubrication theory. A higher-order formulation further improves the accuracy of the results for the pressure drop along the channel.
Hygroscopic Mg–Al LDHs composite microspheres for highly efficient hyperspectral camouflage in the VIS and NIR wavebands
Authors: Xie Zixun , Yuan Le , Qing Xiaolong , Wang Yaqing , Wu Xiaoyan , Weng Xiaolong
Published: July 2024

In order to enhance the hyperspectral camouflage efficacy of stealth coatings against a natural vegetative backdrop, LiCl, known for its significant hygroscopic properties, was incorporated into green Mg–Al layered double hydroxide (Mg–Al LDHs) material. Micron-sized composite microspheres were subsequently synthesized via the spray-drying granulation technique. The structure, morphology, and chemical composition of these microspheres were thoroughly characterized by X-ray diffraction, scanning electron microscopy, laser particle size analysis, nitrogen adsorption–desorption isotherms, and Fourier-transform infrared spectroscopy. The effect of LiCl content on the moisture absorption capacity and near-infrared reflectance spectra of the microspheres was systematically evaluated. We found that incorporating an optimal amount of LiCl into the internal pores of the Mg–Al LDHs microspheres did not compromise their smooth surface morphology and uniform particulate distribution. Notably, when the LiCl content was 10%, the maximum saturation moisture uptake ratio of the coating increased to 0.75 g/g. This hygroscopicity significantly enhanced the absorption and scattering of near-infrared radiation by the coating while concurrently improving its ability to modulate the shape and reflectance of both the visible and near-infrared spectral curves. Spectral congruence between the synthetic coating and natural green foliage was quantified at 97.41%. Moreover, this performance was maintained over 10 cycles of programmed drying and re-humidification, and the coating consistently demonstrated stable hygroscopic properties and sustained over 95% spectral congruence. These optimized artificial coatings were found to effectively confuse hyperspectral classification algorithms, thus blending seamlessly into a natural foliage backdrop. This study provides a new method for regulating VIS and NIR spectral (visible–near infrared spectrum) features, which will be critical for applications in advanced hyperspectral camouflage materials.
Association between exposure to smoke from cooking fuels and anaemia among women of reproductive age in Ghana
Authors: Adarkwa Samuel Akwasi , Oduro Michael Safo , Morgan Anthony Kwame , Arhin-Donkor Seth
Published: July 2024

In low- and middle-income countries, indoor air pollution (IAP) is a serious public health concern, especially for women and children who cook with solid fuels. IAP exposure has been linked to a number of medical conditions, including pneumonia, ischemic heart disease, stroke, chronic obstructive pulmonary disease (COPD), lung cancer, and anaemia. Around 500 million women of reproductive age (WRA) suffer from anaemia globally, with an estimated 190 million cases in sub-Saharan Africa (SSA). This study, which is based on prior research, investigates the relationship between IAP exposure and anaemia among WRA in Ghana. A diverse sample of 2,406 WRA living in Ghana were interviewed, of which 58.06% were anaemic and used high-pollutant fuels for cooking. Age, place of residence, region, education level, religion, ethnicity, wealth index, type of drinking water, type of toilet facility, and type of cooking fuels were all found to be significantly linked with anaemic state by bivariate analysis. Type of cooking fuels utilized, age, region of residence, and the type of residence were shown to be significant predictors of anaemia status using sequential binary logit regression models. The results emphasise the critical need for efforts to promote the usage of clean cooking fuel in an attempt to lower anaemia prevalence in Ghana. To reduce dependency on solid fuels for cooking, initiatives should promote the use of cleaner cooking fuels and enhance the socioeconomic status of households. These interventions could have significant public health effects by reducing the burden of anaemia and improving maternal and child health outcomes due to the prevalence of anaemia among WRA. Overall, this study sheds light on the relationship between IAP exposure and anaemia in Ghana and highlights the demand for focused public health initiatives to address this serious health problem.
A retrospective observational study evaluating the association between vasoactive–inotropic score and mortality after major abdominal surgery
Authors: Huang Jiao , Ji Jiemei , Zhao Yang , Liu Jingchen
Published: July 2024

The relationship between VISmax and mortality in patients undergoing major abdominal surgery remains unclear. This study aims to evaluate the association between VISmax and both short-term and long-term all-cause mortality in patients undergoing major abdominal surgery, VISmax was calculated (VISmax = dopamine dose [µg/kg/min] + dobutamine dose [µg/kg/min] + 100 × epinephrine dose [µg/kg/min] + 10 × milrinone dose [µg/kg/min] + 10,000 × vasopressin dose [units/kg/min] + 100 × norepinephrine dose [µg/kg/min]) using the maximum dosing rates of vasoactives and inotropics within the first 24 h postoperative ICU admission. The study included 512 patients first admitted to the intensive care unit (ICU) who were administered vasoactive drugs after major abdominal surgery. The data was extracted from the medical information mart in intensive care-IV database. VISmax was stratified into five categories: 0–5, > 5–15, > 15–30, > 30–45, and > 45. Compared to patients with the lowest VISmax (≤ 5), those with the high VISmax (> 45) had an increased risk of 30-day mortality (hazard ratio [HR] 3.73, 95% CI 1.16–12.02; P = 0.03) and 1-year mortality (HR 2.76, 95% CI 1.09–6.95; P = 0.03) in fully adjusted Cox models. The ROC analysis for VISmax predicting 30-day and 1-year mortality yielded AUC values of 0.69 (95% CI 0.64–0.75) and 0.67 (95% CI 0.62–0.72), respectively. In conclusion, elevated VISmax within the first postoperative 24 h after ICU admission was associated with increased risks of both short-term and long-term mortality in patients undergoing major abdominal surgery.
Exploring the Loss Aversion Scale’s psychometric properties in Spain
Authors: Cabedo-Peris Javier , Merino-Soto César , Chans Guillermo M. , Martí-Vilar Manuel
Published: July 2024

Loss aversion is a psychological construct defined as a tendency to value potential losses more than gains in a situation that requires decision-making. The Loss Aversion Scale (LAS, eight items) measures an individual’s loss aversion to various situations. However, the generalization of its psychometric properties to different population groups is unknown. This study aimed to validate the LAS instrument for use among Spanish university adults. To this end, two studies were conducted: a content validity study calculating the substantive validity (N = 24) of the instrument’s translation from original English to Spanish and a study of internal structure and association (N = 766) among Spanish university men and women aged 18–35. The analyses performed for each sample indicated that the instrument had adequate validity and reliability values as a one-dimensional measure; however, items 5 and 8 had to be removed. Their scores indicated moderate-magnitude correlations with social desirability. This article debates the study’s limitations, practical implications, and future lines of research based on the results. The conclusion is that the Loss Aversion Scale instrument suits general Spanish population samples and requires probable methodological control concerning social desirability.
Association of platelet to high-density lipoprotein cholesterol ratio with hyperuricemia
Authors: Yan Laisha , Hu Xiaoyan , Wu Shanshan , Zhao Shunying
Published: July 2024

The platelet/high-density lipoprotein ratio (PHR) has been identified as a significant indicator of inflammation and a hypercoagulable state, demonstrating a strong link with the severity of nonalcoholic fatty liver disease (NAFLD) and metabolic syndrome (MetS). However, its correlation with hyperuricemia has not yet been documented. This study utilized a cross-sectional design, analyzing data collected from the National Health and Nutrition Examination Survey (NHANES) between 2007 and 2016 in the United States. The platelet/high-density lipoprotein ratio (PHR) was determined by dividing the number of platelets (PLT) by the level of high-density lipoprotein cholesterol (HDL-C). We employed multivariable logistic regression analyses, generalized additive models, and subgroup analyses to investigate the correlation between PHR and hyperuricemia. The study revealed a hyperuricemia prevalence of 18.56%. Analysis indicated a significant positive correlation between PHR and the risk of hyperuricemia (OR 1.11, 95% CI 1.08, 1.14). This correlation remained consistent across different subgroups including age, ethnicity, gender, and body mass index (BMI). Smooth curve fitting demonstrated a saturation effect between PHR and the risk of hyperuricemia. PHR is positively correlated with hyperuricemia and may serve as a novel biomarker for predicting the onset of this condition. Additionally, targeted interventions to improve PHR might help reduce the incidence of hyperuricemia.
Work autonomy and its associated factors among health professionals in public hospitals of North East Ethiopia
Authors: Yimer Ali , Zewdie Amare , Feleke Amsalu , Dellie Endalkachew , Ahmed Mohammed , Seid Seada , Debebe Wubshet , Ahmed Hassen , Korma Wolyu , Adem Mohammed , Kebede Abdulaziz
Published: July 2024

A low level of work autonomy is the bottleneck for the health service delivery and the quality of the service. Although work autonomy is the pillar of organizational commitment and a means of employee retention mechanism, information about the magnitude of work autonomy among health professionals is limited in Ethiopia. Therefore, this study aimed to assess work autonomy and its predictors among health professionals working in public hospitals of Northeast Ethiopia. Institution-based cross-sectional study was conducted from March 24 to April 24, 2021, among health professionals using a stratified sampling technique. Variables with a p-value of < 0.25 in bivariable analysis were included in the multivariable analysis and variables with a p-value of < 0.05 in multivariable analysis were regarded as significantly associated factors. The overall good work autonomy in public hospitals (Dessie and Boru Meda Hospital) of North East Ethiopia was 54.5% (95% CI 54.48–54.53). Satisfaction with organizational policy and strategy (AOR 2.34, 95% CI 1.29–4.25), satisfaction with supervisor support (AOR 7.20, 95% CI 3.97–13.07), good health service delivery planning practice (AOR 1.88, 95%CI: 1.13–3.13), being married (AOR 4.26, 95%CI: 2.06–8.82) being pharmacy professionals (AOR 0.44, 95% CI 0.19–0.98), and being anesthesia and radiology professionals (AOR 4.66, 95% CI 1.65–13.19) were significantly associated with work autonomy of health professionals. More than half of the health professionals working in public hospitals in Northeast Ethiopia are autonomous in their work. Satisfaction with organizational policy and strategy, satisfaction with supervisor support, having good health service delivery planning practice, being married, and type of profession were significantly associated factors in public hospitals. Thus, strengthening strategies aimed at shaping poor health service delivery planning practices and dissatisfaction of employees concerning supervisor support and organizational policy might have a substantial contribution to improving the work autonomy of health professionals.