bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of Past and Projected Trends of Rainfall and Temperature Parameters in Eastern and Western Hararghe Zones, Ethiopia
Authors: Teshome Helen , Tesfaye Kindie , Dechassa Nigussie , Tana Tamado , Huber Matthew
Published: December 2021

Smallholder farmers in East and West Hararghe zones, Ethiopia frequently face problems of climate extremes. Knowledge of past and projected climate change and variability at local and regional scales can help develop adaptation measures. A study was therefore conducted to investigate the spatio-temporal dynamics of rainfall and temperature in the past (1988–2017) and projected periods of 2030 and 2050 under two Representative Concentration Pathways (RCP4.5 and RCP8.5) at selected stations in East and West Hararghe zones, Ethiopia. To detect the trends and magnitude of change Mann–Kendall test and Sen’s slope estimator were employed, respectively. The result of the study indicated that for the last three decades annual and seasonal and monthly rainfall showed high variability but the changes are not statistically significant. On the other hand, the minimum temperature of the ‘Belg’ season showed a significant (p < 0.05) increment. The mean annual minimum temperature is projected to increase by 0.34 °C and 2.52 °C for 2030, and 0.41 °C and 4.15 °C for 2050 under RCP4.5 and RCP8.5, respectively. Additionally, the mean maximum temperature is projected to change by −0.02 °C and 1.14 °C for 2030, and 0.54 °C and 1.87 °C for 2050 under RCP4.5 and RCP 8.5, respectively. Annual rainfall amount is also projected to increase by 2.5% and 29% for 2030, and 12% and 32% for 2050 under RCP4.5 and RCP 8.5, respectively. Hence, it is concluded that there was an increasing trend in the Belg season minimum temperature. A significant increasing trend in rainfall and temperature are projected compared to the baseline period for most of the districts studied. This implies a need to design climate-smart crop and livestock production strategies, as well as an early warning system to counter the drastic effects of climate change and variability on agricultural production and farmers’ livelihood in the region.
Fifty Years of Development of the Skull Vibration-Induced Nystagmus Test
Authors: Sinno Solara , Schmerber Sébastien , Perrin Philippe , Dumas Georges
Published: December 2021

This review enumerates most of the studies on the Skull Vibration-Induced Nystagmus Test (SVINT) in the past 50 years from different research groups around the world. It is an attempt to demonstrate the evolution of this test and its increased interest around the globe. It explores clinical studies and animal studies, both permitting a better understanding of the importance of SVINT and its pathophysiology.
Automated Detection and Classification of Meningioma Tumor from MR Images Using Sea Lion Optimization and Deep Learning Models
Authors: Sukumaran Aswathy , Abraham Ajith
Published: December 2021

Meningiomas are the most prevalent benign intracranial life-threatening brain tumors, with a life expectancy of a few months in the later stages, so this type of tumor in the brain image should be recognized and detected efficiently. The source of meningiomas is unknown. Radiation exposure, particularly during childhood, is the sole recognized environmental risk factor for meningiomas. The imaging technique of magnetic resonance imaging (MRI) is commonly used to detect most tumor forms as it is a non-invasive and painless method. This study introduces a CNN-HHO integrated automated identification model, which makes use of SeaLion optimization methods for improving overall network optimization. In addition to these techniques, various CNN models such as Resnet, VGG, and DenseNet have been utilized to give an overall influence of CNN with SeaLion in each methodology. Each model is tested on our benchmark dataset for accuracy, specificity, dice coefficient, MCC, and sensitivity, with DenseNet outperforming the other models with a precision of 98%. The proposed methods outperform existing alternatives in the detection of brain tumors, according to the existing experimental findings.
Construction of a High-Density Genetic Linkage Map for the Mapping of QTL Associated with Growth-Related Traits in Sea Cucumber (Apostichopus japonicus)
Authors: Cui Wei , Huo Da , Liu Shilin , Xing Lili , Su Fang , Yang Hongsheng , Sun Lina
Published: December 2021

Genetic linkage maps have become an indispensable tool for genetics and genomics research. Sea cucumber (Apostichopus japonicus), which is an economically important mariculture species in Asia, is an edible echinoderm with medicinal properties. In this study, the first SNP-based high-density genetic linkage map was constructed by sequencing 132 A. japonicus individuals (2 parents and 130 offspring) according to a genotyping-by-sequencing (GBS) method. The consensus map was 3181.54 cM long, with an average genetic distance of 0.52 cM. A total of 6144 SNPs were assigned to 22 linkage groups (LGs). A Pearson analysis and QTL mapping revealed the correlations among body weight, body length, and papillae number. An important growth-related candidate gene, protein still life, isoforms C/SIF type 2 (sif), was identified in LG18. The gene was significantly highly expressed during the larval developmental stages. Its encoded protein reportedly functions as a guanine nucleotide exchange factor. These results would facilitate the genetic analysis of growth traits and provide valuable genomic resources for the selection and breeding of new varieties of sea cucumbers with excellent production traits.
Connexons Coupling to Gap Junction Channel: Potential Role for Extracellular Protein Stabilization Centers
Authors: Héja László , Simon Ágnes , Szabó Zsolt , Kardos Julianna
Published: December 2021

Connexin (Cx) proteins establish intercellular gap junction channels (Cx GJCs) through coupling of two apposed hexameric Cx hemichannels (Cx HCs, connexons). Pre- and post-GJ interfaces consist of extracellular EL1 and EL2 loops, each with three conserved cysteines. Previously, we reported that known peptide inhibitors, mimicking a variety of Cx43 sequences, appear non-selective when binding to homomeric Cx43 vs. Cx36 GJC homology model subtypes. In pursuit of finding potentially Cx subtype-specific inhibitors of connexon-connexon coupling, we aimed at to understand better how the GJ interface is formed. Here we report on the discovery of Cx GJC subtype-specific protein stabilization centers (SCs) featuring GJ interface architecture. First, the Cx43 GJC homology model, embedded in two opposed membrane bilayers, has been devised. Next, we endorsed the fluctuation dynamics of SCs of the interface domain of Cx43 GJC by applying standard molecular dynamics under open and closed cystine disulfide bond (CS-SC) preconditions. The simulations confirmed the major role of the unique trans-GJ SC pattern comprising conserved (55N, 56T) and non-conserved (57Q) residues of the apposed EL1 loops in the stabilization of the GJC complex. Importantly, clusters of SC patterns residing close to the GJ interface domain appear to orient the interface formation via the numerous SCs between EL1 and EL2. These include central 54CS-S198C or 61CS-S192C contacts with residues 53R, 54C, 55N, 197D, 199F or 64V, 191P, respectively. In addition, we revealed that GJC interface formation is favoured when the psi dihedral angle of the nearby 193P residue is stable around 180° and the interface SCs disappear when this angle moves to the 0° to −45° range. The potential of the association of non-conserved residues with SC motifs in connexon-connexon coupling makes the development of Cx subtype-specific inhibitors viable.
Predicting Diagnostic Potential of Cathepsin in Epithelial Ovarian Cancer: A Design Validated by Computational, Biophysical and Electrochemical Data
Authors: Ranade Hemangi , Paliwal Priya , Chaudhary Anis Ahmad , Piplani Sakshi , Rudayni Hassan Ahmed , Al-Zharani Mohammed , Niraj Ravi Ranjan , Datta Manali
Published: December 2021

Background: Epithelial ovarian cancer remains one of the leading variants of gynecological cancer with a high mortality rate. Feasibility and technical competence for screening and detection of epithelial ovarian cancer remain a major obstacle and the development of point of care diagnostics (POCD) may offer a simple solution for monitoring its progression. Cathepsins have been implicated as biomarkers for cancer progression and metastasis; being a protease, it has an inherent tendency to interact with Cystatin C, a cysteine protease inhibitor. This interaction was assessed for designing a POCD module. Methods: A combinatorial approach encompassing computational, biophysical and electron-transfer kinetics has been used to assess this protease-inhibitor interaction. Results: Calculations predicted two cathepsin candidates, Cathepsin K and Cathepsin L based on their binding energies and structural alignment and both predictions were confirmed experimentally. Differential pulse voltammetry was used to verify the potency of Cathepsin K and Cathepsin L interaction with Cystatin C and assess the selectivity and sensitivity of their electrochemical interactions. Electrochemical measurements indicated selectivity for both the ligands, but with increasing concentrations, there was a marked difference in the sensitivity of the detection. Conclusions: This work validated the utility of dry-lab integration in the wet-lab technique to generate leads for the design of electrochemical diagnostics for epithelial ovarian cancer.
Association of Oxidative Stress Markers with Vascular Stiffness Parameters in Patients with Diabetic Neuropathy
Authors: Mačáková Dominika , Plchová Markéta , Cibičková Lubica , Krystyník Ondřej , Karásek David , Zadrazil Josef
Published: December 2021

Introduction: One of the most common chronic complications of diabetes mellitus is diabetic neuropathy. The aim of the study was to elucidate the association between selected markers of oxidative stress and markers of vascular stiffness and to contribute to the understanding of the pathophysiological links between oxidative stress and micro- and macrovascular complications of diabetes. Methods: We enrolled patients with type 2 DM (n = 49), with moderate to severe diabetic polyneuropathy of lower extremities, and a control group without microvascular complications (n = 29). The neuropathy group received alpha-lipoic acid infusion therapy. Sampling was performed before and after treatment to determine the level of oxidative markers (advanced glycation end-products—AGEs, glycation products of AOPP proteins, MDA malondialdehyde and oxidized LDL), parameters of metabolic control and parameters of vascular wall stiffness were measured by sphygmomanometry. Results: After the administration of alpha-lipoic acid, we demonstrated a significant reduction in the level of three selected oxidation markers (AOPP: p < 0.001, AGE: p < 0.001, oxLDL: p < 0.05). In contrast, the level of MDA did not change significantly (p = 0.83). Throughout the group, oxLDL was significantly correlated with central BP (SBP and DBP in the aorta, p < 0.05 and <0.01) and with the augmentation index (AiX/75 bpm, p < 0.01). AOPP significantly correlated with systolic BP in the aorta (p < 0.05). We did not find significant associations in the remaining oxidation markers. Conclusion: In our study, we demonstrated a reduction in the level of oxidative markers after alpha-lipoic acid administration and also an association between markers of oxidative damage to lipids and proteins (oxLDL and AOPP) and some parameters of vascular stiffness.
The Relationship between Telomere Length and Gestational Weight Gain: Findings from the Mamma & Bambino Cohort
Authors: Maugeri Andrea , Magnano San Lio Roberta , La Rosa Maria Clara , Giunta Giuliana , Panella Marco , Cianci Antonio , Caruso Maria Anna Teresa , Agodi Antonella , Barchitta Martina
Published: December 2021

Inadequate gestational weight gain (GWG) affects a growing number of pregnancies, influencing intrauterine environment and long-term health. Uncovering molecular mechanisms associated with GWG could be helpful to develop public health strategies for tackling this issue. Here, our study aimed to understand the relationship of DNA telomere length with weigh gain during pregnancy, using data and samples from the ongoing prospective “Mamma & Bambino” study (Catania, Italy). GWG was calculated according to the Institute of Medicine (IOM) guidelines. Relative telomere length was assessed by real-time quantitative polymerase chain reaction in 252 samples of maternal leucocyte DNA (mlDNA) and 150 samples of cell-free DNA (cfDNA) from amniotic fluid. We observed that relative telomere length of mlDNA seemed to weakly increase with GWG. In contrast, telomere length of cfDNA exhibited a U-shaped relationship with GWG. Women with adequate GWG showed longer telomere length than those who gained weight inadequately. Accordingly, the logistic regression model confirmed the association between telomere length of cfDNA and adequate GWG, after adjusting for potential confounders. Our findings suggest an early effect of GWG on telomere length of cfDNA, which could represent a molecular mechanism underpinning the effects of maternal behaviours on foetal well-being.
Neutrophil to Lymphocyte Ratio (NLR)—A Useful Tool for the Prognosis of Sepsis in the ICU
Authors: Drăgoescu Alice Nicoleta , Pădureanu Vlad , Stănculescu Andreea Doriana , Chiuțu Luminița Cristina , Tomescu Paul , Geormăneanu Cristiana , Pădureanu Rodica , Iovănescu Vlad Florin , Ungureanu Bogdan Silviu , Pănuș Andrei , Drăgoescu Octavian Petru
Published: December 2021

Sepsis is a life-threatening medical emergency induced by the body′s extreme response to an infection. Despite well-defined and constantly updated criteria for diagnosing sepsis, it is still underdiagnosed worldwide. Among various markers studied over time, the neutrophil to lymphocyte ratio (NLR) recently emerged as a good marker to predict sepsis severity. Our study was a single-center prospective observational study performed in our ICU and included 114 patients admitted for sepsis or septic shock. Neutrophil to lymphocyte ratio (NLR) is easy to perform, CBC being one of the standard blood tests routinely performed upon admission for all ICU patients. We found that NLR was increased in all patients with sepsis and significantly raised in those with septic shock. NLR correlates significantly with sepsis severity evaluated by the SOFA score (R = 0.65) and also with extensively studied sepsis prognosis marker presepsin (R = 0.56). Additionally, NLR showed good sensitivity (47%) and specificity (78%) with AUC = 0.631 (p < 0.05). NLR is less expensive and easier to perform compared with other specific markers and may potentially become a good alternate option for evaluation of sepsis severity. Larger studies are needed in the future to demonstrate the prognosis value of NLR.
Neurogenic Inflammation: The Participant in Migraine and Recent Advancements in Translational Research
Authors: Spekker Eleonóra , Tanaka Masaru , Szabó Ágnes , Vécsei László
Published: December 2021

Migraine is a primary headache disorder characterized by a unilateral, throbbing, pulsing headache, which lasts for hours to days, and the pain can interfere with daily activities. It exhibits various symptoms, such as nausea, vomiting, sensitivity to light, sound, and odors, and physical activity consistently contributes to worsening pain. Despite the intensive research, little is still known about the pathomechanism of migraine. It is widely accepted that migraine involves activation and sensitization of the trigeminovascular system. It leads to the release of several pro-inflammatory neuropeptides and neurotransmitters and causes a cascade of inflammatory tissue responses, including vasodilation, plasma extravasation secondary to capillary leakage, edema, and mast cell degranulation. Convincing evidence obtained in rodent models suggests that neurogenic inflammation is assumed to contribute to the development of a migraine attack. Chemical stimulation of the dura mater triggers activation and sensitization of the trigeminal system and causes numerous molecular and behavioral changes; therefore, this is a relevant animal model of acute migraine. This narrative review discusses the emerging evidence supporting the involvement of neurogenic inflammation and neuropeptides in the pathophysiology of migraine, presenting the most recent advances in preclinical research and the novel therapeutic approaches to the disease.
Multiple Chemical Sensitivity
Authors: Zucco Gesualdo M. , Doty Richard L.
Published: December 2021

Multiple Chemical Sensitivity (MCS), a condition also known as Chemical Sensitivity (CS), Chemical Intolerance (CI), Idiopathic Environmental Illness (IEI) and Toxicant Induced Loss of Tolerance (TILT), is an acquired multifactorial syndrome characterized by a recurrent set of debilitating symptoms. The symptoms of this controversial disorder are reported to be induced by environmental chemicals at doses far below those usually harmful to most persons. They involve a large spectrum of organ systems and typically disappear when the environmental chemicals are removed. However, no clear link has emerged among self-reported MCS symptoms and widely accepted objective measures of physiological dysfunction, and no clear dose-response relationship between exposure and symptom reactions has been observed. In addition, the underlying etiology and pathogenic processes of the disorder remain unknown and disputed, although biologic and psychologic hypotheses abound. It is currently debated whether MCS should be considered a clinical entity at all. Nevertheless, in the last few decades MCS has received considerable scientific and governmental attention in light of the many persons reporting this illness. In this review, we provide a general overview of the history, definition, demographics, prevalence, and etiologic challenges in defining and understanding MCS.
Repetitive Transcranial Magnetic Stimulation for Comorbid Major Depressive Disorder and Alcohol Use Disorder
Authors: Tang Victor M. , Le Foll Bernard , Blumberger Daniel M. , Voineskos Daphne
Published: December 2021

Major depressive disorder (MDD) and alcohol use disorder (AUD) are leading causes of disability, and patients are frequently affected by both conditions. This comorbidity is known to confer worse outcomes and greater illness severity. Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive neuromodulation method that has demonstrated antidepressant effects. However, the study of rTMS for patients with MDD and commonly associated comorbidities, such as AUD, has been largely overlooked, despite significant overlap in clinical presentation and neurobiological mechanisms. This narrative review aims to highlight the interrelated aspects of the literature on rTMS for MDD and rTMS for AUD. First, we summarize the available evidence on the effectiveness of rTMS for each condition, both most studied through stimulation of the dorsolateral prefrontal cortex (DLPFC). Second, we describe common symptom constructs that can be modulated by rTMS, such as executive dysfunction, that are transdiagnostic across these disorders. Lastly, we describe promising approaches in the personalization and optimization of rTMS that may be applicable to both AUD and MDD. By bridging the gap between research efforts in MDD and AUD, rTMS is well positioned to be developed as a treatment for the many patients who have both conditions concurrently.
Support Needs and Interventions for Family Caregivers of Patients with Amyotrophic Lateral Sclerosis (ALS): A Narrative Review with Report of Telemedicine Experiences at the Time of COVID-19 Pandemic
Authors: D’Alvano Giulia , Buonanno Daniela , Passaniti Carla , De Stefano Manuela , Lavorgna Luigi , Tedeschi Gioacchino , Siciliano Mattia , Trojsi Francesca
Published: December 2021

Family caregivers of people with amyotrophic lateral sclerosis (ALS), a severely disabling neurodegenerative disease due to the degeneration of both upper and lower motor neurons, have a very demanding role in managing their relatives, thereby often experiencing heavy care burden. Previous literature has widely highlighted that this situation reduces caregivers’ quality of life and increases their psychological distress and risk of health problems, but there are relatively few studies that focus on psychological interventions for these situations. Family support is more—not less—important during crisis. However, during the COVID-19 pandemic, maintaining public safety has required restricting the physical presence of families for hospitalized patients. Caregivers of ALS patients felt increased sense of loneliness and experienced greater difficulties in the access to both hospital and home assistance. In response, health systems rapidly adapted family-centric procedures and tools to circumvent restrictions on physical presence. In this regard, internet-based and telehealth solutions have been adopted to facilitate the routine, predictable, and structured communication, crucial to family-centered care. This narrative review aims at addressing more current matters on support needs and interventions for improving wellbeing of caregivers of ALS patients. In particular, we aimed at highlighting several gaps related to the complex needs of caregivers of ALS patients, to the interventions carried out in order to respond to these needs, and to the changes that COVID-19 pandemic caused from 2020 to nowadays in clinical managing of ALS patients. Finally, we report ongoing experiences of psychological support for family caregivers of ALS patients through telehealth solutions, which have been reinforced in case of needing of physical distancing during the COVID-19 pandemic.
Cognitive Control in Young and Older Adults: Does Mood Matter?
Authors: Truong Linda , Kandasamy Kesaan , Yang Lixia
Published: December 2021

The dual mechanisms of control framework (DMC) proposes two modes of cognitive control: proactive and reactive control. In anticipation of an interference event, young adults primarily use a more proactive control mode, whereas older adults tend to use a more reactive one during the event, due to age-related deficits in working memory. The current study aimed to examine the effects of mood induction on cognitive control mode in older (ages 65+) compared to young adults (ages 18–30) with a standard letter-cue (Experiment 1) and a modified face-cue AX-CPT (Experiment 2). Mood induction into negative and/or positive mood versus neutral mood was conducted prior to the cognitive control task. Experiment 1 replicated the typical pattern of proactive control use in young adults and reactive control use in older adults. In Experiment 2, older adults showed comparable proactive control to young adults in their response time (RT). Mood induction showed little effect on cognitive control across the two experiments. These results did not reveal consistent effects of mood (negative or positive) on cognitive control mode in young and older adults, but discovered (or demonstrated) that older adults can engage proactive control when dichotomous face cues (female or male) are used in AX-CPT.
Saturation Transfer MRI for Detection of Metabolic and Microstructural Impairments Underlying Neurodegeneration in Alzheimer’s Disease
Authors: Orzyłowska Anna , Oakden Wendy
Published: December 2021

Alzheimer’s disease (AD) is one of the most common causes of dementia and difficult to study as the pool of subjects is highly heterogeneous. Saturation transfer (ST) magnetic resonance imaging (MRI) methods are quantitative modalities with potential for non-invasive identification and tracking of various aspects of AD pathology. In this review we cover ST-MRI studies in both humans and animal models of AD over the past 20 years. A number of magnetization transfer (MT) studies have shown promising results in human brain. Increased computing power enables more quantitative MT studies, while access to higher magnetic fields improves the specificity of chemical exchange saturation transfer (CEST) techniques. While much work remains to be done, results so far are very encouraging. MT is sensitive to patterns of AD-related pathological changes, improving differential diagnosis, and CEST is sensitive to particular pathological processes which could greatly assist in the development and monitoring of therapeutic treatments of this currently incurable disease.
Robotic-Assisted vs. Standard Laparoscopic Surgery for Rectal Cancer Resection: A Systematic Review and Meta-Analysis of 19,731 Patients
Authors: Safiejko Kamil , Tarkowski Radoslaw , Koselak Maciej , Juchimiuk Marcin , Tarasik Aleksander , Pruc Michal , Smereka Jacek , Szarpak Lukasz
Published: December 2021

Robotic-assisted surgery is expected to have advantages over standard laparoscopic approach in patients undergoing curative surgery for rectal cancer. PubMed, Cochrane Library, Web of Science, Scopus and Google Scholar were searched from database inception to 10 November 2021, for both RCTs and observational studies comparing robotic-assisted versus standard laparoscopic surgery for rectal cancer resection. Where possible, data were pooled using random effects meta-analysis. Forty-Two were considered eligible for the meta-analysis. Survival to hospital discharge or 30-day overall survival rate was 99.6% for RG and 98.8% for LG (OR = 2.10; 95% CI: 1.00 to 4.43; p = 0.05). Time to first flatus in the RG group was 2.5 ± 1.4 days and was statistically significantly shorter than in LG group (2.9 ± 2.0 days; MD = −0.34; 95%CI: −0.65 to 0.03; p = 0.03). In the case of time to a liquid diet, solid diet and bowel movement, the analysis showed no statistically significant differences (p > 0.05). Length of hospital stay in the RG vs. LG group varied and amounted to 8.0 ± 5.3 vs. 9.5 ± 10.0 days (MD = −2.01; 95%CI: −2.90 to −1.11; p < 0.001). Overall, 30-days complications in the RG and LG groups were 27.2% and 19.0% (OR = 1.11; 95%CI: 0.80 to 1.55; p = 0.53), respectively. In summary, robotic-assisted techniques provide several advantages over laparoscopic techniques in reducing operative time, significantly lowering conversion of the procedure to open surgery, shortening the duration of hospital stay, lowering the risk of urinary retention, improving survival to hospital discharge or 30-day overall survival rate.
Enhanced Adhesion Strength of Pt/γ-Al2O3 Catalysts on STS-444 Substrate via γ-Al2O3 Intermediate Layer Formation: Application for CO and C3H6 Oxidation
Authors: Ryu Seung-Hee , Hwang Cheol Hong , Jeong Hojin , Kim Giyeong , Ahn Sung Il , Park Joon Sik , Choi Joon-Hwan
Published: December 2021

Pt/γ-Al2O3 catalysts coated on honeycomb-shaped stainless STS-444 steel substrates with a γ-Al2O3 intermediate layer were prepared using a conventional washcoating method. The intermediate layer was formed on the substrate surface through oxidation using pack cementation. The monolithic catalysts with the intermediate layer were fabricated for potential applications to pre-turbocharger catalysts, which suffer from severe conditions such as vibrations of the engine and high flow rates of exhaust gas. Adhesive strength tests and simultaneous oxidation reactions of CO and C3H6 were carried out for the Pt/γ-Al2O3 monolithic catalysts with and without the intermediate layer. The catalysts with an intermediate layer showed much stronger adhesion than the catalysts without an intermediate layer. Thus, the formation of a γ-Al2O3 intermediate layer by surface oxidation through pack cementation facilitated a significant enhancement of the catalyst adhesion strength without catalytic performance degradation.
Hierarchical Graphitic Carbon-Encapsulating Cobalt Nanoparticles for Catalytic Hydrogenation of 2,4-Dinitrophenol
Authors: Hammud Hassan H. , Traboulsi Hassan , Karnati Ranjith Kumar , Hussain Syed Ghazanfar , Bakir Esam M.
Published: December 2021

Cobalt hierarchical graphitic carbon nanoparticles (Co@HGC) (1), (2), and (3) were prepared by simple pyrolysis of a cobalt phenanthroline complex in the presence of anthracene at different temperatures and heating times, under a nitrogen atmosphere. The samples were used for the catalytic hydrogenation of 2,4-dinitrophenol. Samples (1) and (3) were prepared by heating at 600 °C and 800 °C respectively, while (2) was prepared by heating at 600 °C with an additional intermediate stage at 300 °C. This work revealed that graphitization was catalyzed by cobalt nanoparticles and occurred readily at temperatures of 600 °C and above. The nanocatalysts were characterized by Scanning Electron Microscopy SEM, energy dispersive X-ray analysis EDX, Raman, Xrd, and XPS. The analysis revealed the presence of cobalt and cobalt oxide species as well as graphitized carbon, while TEM analysis indicated that the nanocatalyst contains mainly cobalt nanoparticles of 3–20 nm in size embedded in a lighter graphitic web. Some bamboo-like multiwall carbon nanotubes and graphitic onion-like nanostructures were observed in (3). The structures and chemical properties of the three catalysts were correlated with their catalytic activities. The apparent rate constants kapp (min−1) of the 2,4-dinitrophenol reductions were 0.34 for (2), 0.17 for (3), 0.04 for (1), 0.005 (no catalyst). Among the three studied catalysts, the highest rate constant was obtained for (2), while the highest conversion yield was achieved by (3). Our data show that an increase in agglomeration of the cobalt species reduces the catalytic activity, while an increase in pyrolysis temperature improves the conversion yield. The nanocatalyst enhances hydrogen generation in the presence of sodium borohydride and reduces 2,4-dinitrophenol to p-diamino phenol. The best nanocatalyst (3) was prepared at 800 °C. It consisted of uniformly distributed cobalt nanoparticles sheltered by hierarchical graphitic carbon. The nanocatalyst is easily separated and recycled from the reaction system and proved to be degradation resistant, to have robust stability, and high activity towards the reduction reaction of nitrophenols.
Stem Cell-Based Disease Models for Inborn Errors of Immunity
Authors: Zbinden Aline , Canté-Barrett Kirsten , Pike-Overzet Karin , Staal Frank J. T.
Published: December 2021

The intrinsic capacity of human hematopoietic stem cells (hHSCs) to reconstitute myeloid and lymphoid lineages combined with their self-renewal capacity hold enormous promises for gene therapy as a viable treatment option for a number of immune-mediated diseases, most prominently for inborn errors of immunity (IEI). The current development of such therapies relies on disease models, both in vitro and in vivo, which allow the study of human pathophysiology in great detail. Here, we discuss the current challenges with regards to developmental origin, heterogeneity and the subsequent implications for disease modeling. We review models based on induced pluripotent stem cell technology and those relaying on use of adult hHSCs. We critically review the advantages and limitations of current models for IEI both in vitro and in vivo. We conclude that existing and future stem cell-based models are necessary tools for developing next generation therapies for IEI.
Mitochondrial Dysfunction as a Hallmark of Environmental Injury
Authors: Duarte-Hospital Carolina , Tête Arnaud , Brial François , Benoit Louise , Koual Meriem , Tomkiewicz Céline , Kim Min Ji , Blanc Etienne B. , Coumoul Xavier , Bortoli Sylvie
Published: December 2021

Environmental factors including diet, sedentary lifestyle and exposure to pollutants largely influence human health throughout life. Cellular and molecular events triggered by an exposure to environmental pollutants are extremely variable and depend on the age, the chronicity and the doses of exposure. Only a fraction of all relevant mechanisms involved in the onset and progression of pathologies in response to toxicants has probably been identified. Mitochondria are central hubs of metabolic and cell signaling responsible for a large variety of biochemical processes, including oxidative stress, metabolite production, energy transduction, hormone synthesis, and apoptosis. Growing evidence highlights mitochondrial dysfunction as a major hallmark of environmental insults. Here, we present mitochondria as crucial organelles for healthy metabolic homeostasis and whose dysfunction induces critical adverse effects. Then, we review the multiple mechanisms of action of pollutants causing mitochondrial toxicity in link with chronic diseases. We propose the Aryl hydrocarbon Receptor (AhR) as a model of “exposome receptor”, whose activation by environmental pollutants leads to various toxic events through mitochondrial dysfunction. Finally, we provide some remarks related to mitotoxicity and risk assessment.
Cell-Based Therapy for the Treatment of Glioblastoma: An Update from Preclinical to Clinical Studies
Authors: Attia Noha , Mashal Mohamed , Pemminati Sudhakar , Omole Adekunle , Edmondson Carolyn , Jones Will , Priyadarshini Priyanka , Mughal Temoria , Aziz Pauline , Zenick Blesing , Perez Ambar , Lacken Morgan
Published: December 2021

Glioblastoma (GB), an aggressive primary tumor of the central nervous system, represents about 60% of all adult primary brain tumors. It is notorious for its extremely low (~5%) 5-year survival rate which signals the unsatisfactory results of the standard protocol for GB therapy. This issue has become, over time, the impetus for the discipline of bringing novel therapeutics to the surface and challenging them so they can be improved. The cell-based approach in treating GB found its way to clinical trials thanks to a marvelous number of preclinical studies that probed various types of cells aiming to combat GB and increase the survival rate. In this review, we aimed to summarize and discuss the up-to-date preclinical studies that utilized stem cells or immune cells to treat GB. Likewise, we tried to summarize the most recent clinical trials using both cell categories to treat or prevent recurrence of GB in patients. As with any other therapeutics, cell-based therapy in GB is still hampered by many drawbacks. Therefore, we highlighted several novel techniques, such as the use of biomaterials, scaffolds, nanoparticles, or cells in the 3D context that may depict a promising future when combined with the cell-based approach.
Structure and Properties of Highly Porous Alumina-Based Ceramic Materials after Heating by Concentrated Solar Radiation
Authors: Babashov Vladimir G. , Suleimanov Sultan Kh. , Daskovskii Mikhail I. , Shein Evgeny A. , Stolyankov Yurii V.
Published: December 2021

Three ceramic fibrous materials of the Al2O3-SiO2 system with different densities have been treated using concentrated solar radiation. The experiment was performed using technological capabilities of the Big Solar Furnace in the 2 modes: the first mode includes heating up to 1400–1600 °C, holding for 1.5–2 h; the second mode (the fusion mode) includes heating up to 1750–1900 °C until the sample destruction, which is accompanied by fusion. Upon completion of the experiment, the phase composition, microstructure, and compressive strength of the materials were studied. It was shown that the investigated materials retained their fibrous structure under prolonged treatment in the first mode up to temperatures of 1600 °C. The phase composition of the ceramic materials changes during the experiment, and with a decrease in the density, the modification is more pronounced. Treatment of all three materials under study in the fusion mode resulted in the formation of the eutectic component in the form of spherulites. The compressive strength of the materials was found to be slightly reduced after exposure to concentrated solar radiation.
Incidence of Neonatal Abstinence Syndrome (NAS) in Castilla y Leon (Spain)
Authors: Moreno-Ramos Miriam , Sánchez-Barba Mercedes , Sánchez Rubén García , Mirón-Canelo José Antonio , González-Nuñez Veronica
Published: December 2021

Introduction: Neonatal Abstinence Syndrome (NAS) is considered a Public Health problem that is defined as a group of symptoms that appear in the newborn due to withdrawal from intrauterine drug exposure. Objective: The aim of this study was to analyze the incidence of NAS in Castilla y León from 2000 to 2019. Methodology: Data of NAS cases in Castilla y León from 2000 to 2019 were obtained. NAS incidence per 1000 births was calculated and the correlation among data from different provinces and years was analyzed. Results: The cumulative incidence of NAS in Castilla y León per 1000 births between 2000-2019 was 0.91‰, with great interprovincial variability. The provinces of Zamora and Palencia stand out, with high incidence rates of NAS despite their low birth rates. The temporal trend points towards a decrease in incidence from 2000 to 2019. Opioids such as methadone, cannabis, benzodiazepines and poly-drug use are the most prevalent drugs causing NAS, and it has also been observed that methadone is being replaced by cannabis as the major cause of NAS cases at the University Hospital in Salamanca in recent years. Conclusions: The incidence of NAS in Castilla y León decreased in 2004 and remained constant until 2019, but it shows great interprovincial variability. It is necessary to implement a national NAS Registry to obtain comprehensive information regarding its incidence.
Comparative Analysis of the Transcriptomes of Persisting and Abscised Fruitlets: Insights into Plant Hormone and Carbohydrate Metabolism Regulated Self-Thinning of Pecan Fruitlets during the Early Stage
Authors: Zhang Jiyu , Wang Tao , Zhang Fan , Liu Yongzhi , Wang Gang
Published: December 2021

Pecan is one of the most popular nut species in the world. The fruit drop rate of the pecan ‘Pawnee’ is more than 57%, with four fruit drop stages, which is very serious. In this study, we conducted transcriptomic profiling of persisting and abscised fruitlets in early fruit development by RNA-seq. A total of 11,976 differentially expressed genes (DEGs) were identified, 3012 upregulated and 8964 downregulated, in a comparison of abscised vs. persisting fruitlets at 35 days after anthesis (DAA). Our transcriptomic data suggest that gene subsets encoding elements involving the biosynthesis, metabolism, perception, signal transduction, and crosstalk of the plant hormones abscisic acid (ABA), auxin, cytokinin, ethylene, and gibberellin (GA) and plant growth regulators jasmonates, salicylic acid, and brassinosteroids were differentially expressed. In addition, the majority of transcriptionally activated genes involved in hormone signaling (except for ethylene and salicylic acid signaling) were downregulated in abscised fruitlets. The differential expression of transcripts coding for enzymes involved in sucrose, glucose, trehalose, starch, galactose, and galactinol metabolism shows that sucrose, galactinol, and glucose synthesis and starch content were reduced as starch biosynthesis was blocked, and retrogradation and degradation intensified. These results suggest that the abscised pecan fruitlets stopped growing and developing for some time before dropping, further indicating that their sugar supply was reduced or stopped. The transcriptome characterization described in this paper contributes to unravelling the molecular mechanisms and pathways involved in the physiological abscission of pecan fruits.
Influence of Organic Matter/Bacteria on the Formation and Transformation of Sulfate Green Rust
Authors: Duboscq Julien , Vincent Julia , Jeannin Marc , Sabot René , Lanneluc Isabelle , Sablé Sophie , Refait Philippe
Published: December 2021

The corrosion processes of carbon steel immersed in natural seawater are influenced by microorganisms due to important biological activity. An analysis of the corrosion product layers formed on carbon steel coupons in natural or artificial seawater revealed that sulfate green rust GR(SO42−) was favored in natural environments. In this paper, the role of organic matter/bacteria on the formation and transformation of this compound are addressed. GR(SO42−) was precipitated from Fe(II) and Fe(III) salts in the presence of various marine bacterial species not involved in the redox cycle of Fe or S. Abiotic experiments were performed for comparison, first without any organic species and then with sodium acetate added as a small organic ion. The obtained aqueous suspensions were aged at room temperature for 1 week. The number of bacteria (CFU/mL) was followed over time and the solid phases were characterized by XRD. Whatever the fate of the bacteria (no activity, or activity and growth), the formation of GR(SO42−) was favored and its transformation to magnetite completely inhibited. This effect is attributed to the adsorption of organic molecules on the lateral sides of the GR(SO42−) crystals. A similar effect, though less important, was observed with acetate.
Preliminary Investigation on Degradation Behavior and Cytocompatibility of Ca-P-Sr Coated Pure Zinc
Authors: Ke Mingfeng , Xie Danhua , Tang Qiangqiang , Su Shenghui
Published: December 2021

Zinc and its alloys show a good application prospect as a new biodegradable material. However, one of the drawbacks is that Zn and its alloys would induce the release of more Zn ions, which are reported to be cytotoxic to cells. In this study, a Ca-P-Sr bioactive coating was prepared on the surface of pure zinc by the hydrothermal method to address this issue. The morphology, thickness, and composition were characterized, and the effects of the coating on the degradation, cell viability, and ALP staining were investigated. The results demonstrated that the degradation rate of pure zinc was reduced, while the cytocompatibility was significantly improved after pure zinc was treated with Ca-P-Sr coating. It is considered that the Ca-P-Sr bioactive coating prepared by the hydrothermal method has promising application in the clinic.
Structural and In Situ X-ray Diffraction Study of Hydrogenation of CaxMg1−xNi2 (0 ≤ x ≤ 1)
Authors: Rehman Zia Ur , Nawaz Mohsan , Ullah Hameed , Ahmad Pervaiz , Khandaker Mayeen Uddin , Idris Abubakr M. , Haq Sirajul , Sulieman Abdelmoneim
Published: December 2021

In the quasi-binary system CaNi2-MgNi2 solid-solutions CaxMg1−xNi2 (0 ≤ x ≤ 1) were prepared from the elements. They crystallize in the hexagonal Laves phase type (MgNi2, C36) for x ≤ 0.33 (P63/mmc, a = 482.51(7) pm, c = 1582.1(3) pm for x = 0, a = 482.59(3), c = 1583.1(1) for x = 0.33) and in the cubic Laves phase type (MgCu2, C15) for 0.33 < x (Fd3¯m, a = 697.12(3) pm for x = 0.5, a = 705.11(2) pm for x = 0.67, a = 724.80(2) pm for x = 1). After hydrogenation in an autoclave the X-ray diffraction patterns changed completely. Reflections assigned to CaNiH3, and Ni and Rietveld refinement confirmed this. The hydrogenation properties of CaxMg1−xNi2 (0 ≤ x ≤ 1) compounds were also studied in situ by X-ray powder diffraction. In situ X-ray powder diffraction of CaxMg1−xNi2 (0 ≤ x ≤ 1) compounds under 0.3 MPa hydrogen gas flow (15 sccm), data collected on a Rigaku SmartLab diffractometer in an Anton Paar XRK 900 Reactor Chamber using Cu-Kα1 radiation. Scanning electron microscopy and EDX spectroscopy confirmed the entitled materials and elemental composition, respectively. From the Transmission electron microscopy and Selected area electron diffraction concluded that the CaxMg1−xNi2 (0 ≤ x ≤ 1) compounds were crystalline.
Rietveld Refinement and X-ray Absorption Study on the Bonding States of Lanthanum-Based Perovskite-Type Oxides La1−xCexCoO3
Authors: Nguyen Duc-Chau , Chu Chia-Chin , Anbalagan Aswin Kumar , Lee Chih-Hao , Chang Chia-Seng
Published: December 2021

Metal-oxygen bonding of the Ce-doped LaCoO3 system remains largely unexplored despite extensive studies on its magnetic properties. Here, we investigate the structure and local structure of nanoscale La1−xCexCoO3, with x = 0, 0.2, and 0.4, using the Rietveld refinement and synchrotron X-ray absorption techniques, complemented by topological analysis of experimental electron density and electron energy distribution. The Rietveld refinement results show that LaCoO3 subject to Ce addition is best interpretable by a model of cubic symmetry in contrast to the pristine LaCoO3, conventionally described by either a monoclinic model or a rhombohedral model. Ce4+/Co2+ are more evidently compatible dopants than Ce3+ for insertion into the main lattice. X-ray absorption data evidence the partially filled La 5d-band of the pristine LaCoO3 in accordance with the presence of La–O bonds with the shared-type atomic interaction. With increasing x, the increased Ce spectroscopic valence and enhanced La–O ionic bonding are noticeable. Characterization of the local structures around Co species also provides evidence to support the findings of the Rietveld refinement analysis.
A Comparison of Magnetic Resonance Imaging Methods to Assess Multiple Sclerosis Lesions: Implications for Patient Characterization and Clinical Trial Design
Authors: Haacke Ewart Mark , Bernitsas Evanthia , Subramanian Karthik , Utriainen David , Palutla Vinay Kumar , Yerramsetty Kiran , Kumar Prashanth , Sethi Sean K. , Chen Yongsheng , Latif Zahid , Jella Pavan , Gharabaghi Sara , Wang Ying , Zhang Xiaomeng , Comley Robert A. , Beaver John , Luo Yanping
Published: December 2021

Magnetic resonance imaging (MRI) is a sensitive imaging modality for identifying inflammatory and/or demyelinating lesions, which is critical for a clinical diagnosis of MS and evaluating drug responses. There are many unique means of probing brain tissue status, including conventional T1 and T2 weighted imaging (T1WI, T2WI), T2 fluid attenuated inversion recovery (FLAIR), magnetization transfer, myelin water fraction, diffusion tensor imaging (DTI), phase-sensitive inversion recovery and susceptibility weighted imaging (SWI), but no study has combined all of these modalities into a single well-controlled investigation. The goals of this study were to: compare different MRI measures for lesion visualization and quantification; evaluate the repeatability of various imaging methods in healthy controls; compare quantitative susceptibility mapping (QSM) with myelin water fraction; measure short-term longitudinal changes in the white matter of MS patients and map out the tissue properties of the white matter hyperintensities using STAGE (strategically acquired gradient echo imaging). Additionally, the outcomes of this study were anticipated to aid in the choice of an efficient imaging protocol reducing redundancy of information and alleviating patient burden. Of all the sequences used, T2 FLAIR and T2WI showed the most lesions. To differentiate the putative demyelinating lesions from inflammatory lesions, the fusion of SWI and T2 FLAIR was used. Our study suggests that a practical and efficient imaging protocol combining T2 FLAIR, T1WI and STAGE (with SWI and QSM) can be used to rapidly image MS patients to both find lesions and study the demyelinating and inflammatory characteristics of the lesions.
Role and Relevance of Cerebrospinal Fluid Cells in Diagnostics and Research: State-of-the-Art and Underutilized Opportunities
Authors: Otto Ferdinand , Harrer Christine , Pilz Georg , Wipfler Peter , Harrer Andrea
Published: December 2021

Cerebrospinal fluid (CSF) has recently experienced a revival in diagnostics and research. However, little progress has been made regarding CSF cell analysis. For almost a century, CSF cell count and cytomorphological examination have been central diagnostic parameters, with CSF pleocytosis as a hallmark finding of neuroinflammation and cytology offering valuable clues regarding infectious, autoimmune, and malignant aetiologies. A great deal of information, however, remains unattended as modern immune phenotyping technologies have not yet been broadly incorporated into routine CSF analysis. This is a serious deficit considering the central role of CSF cells as effectors in central nervous system (CNS) immune defence and autoimmune CNS processes, and the diagnostic challenges posed by clinically overlapping infectious and immune-mediated CNS diseases. Here, we summarize historical, specimen-intrinsic, methodological, and technical issues determining the state-of-the-art diagnostics of CSF cells and outline future perspectives for this underutilized window into meningeal and CNS immunity.