bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Rice Planting on Nitrous Oxide (N2O) Emission under Different Levels of Nitrogen Fertilization
Authors: Kim Gil Won , Kim Pil Joo , Khan Muhammad Israr , Lee Sung-Jae
Published: January 2021

Nitrogen (N) fertilization is one of the most effective practices to increase productivity, and has therefore had a fast global increase. Consequently, the effects of the application of N fertilizer on emissions of N2O have been widely studied, but the effect of rice planting on N2O emission was not adequately quantified. To evaluate the effect of rice cultivation on N2O emissions, different levels of N were applied in a typical temperate rice field, and the N2O fluxes were compared in rice-planted and non-planted soils. Seasonal N2O fluxes responded differently with respect to N fertilization in the two different soil conditions. In non-planted soils, seasonal N2O fluxes ranged within 0.31–0.34 kg N2O ha−1 under 0 kg N ha−1 fertilization, and significantly increased by increasing N fertilization rates, with an average rate of 0.0024 kg N2O kg−1 N for 3 years. In rice-planted soils, seasonal N2O fluxes were also increased by N fertilization but showed large negative N2O fluxes, irrespective of the N fertilization level. This study confirms that the rice reacted as a reducer of N2O emissions, not an emission source, in paddy fields, suggesting that N2O fluxes should be estimated by the static chamber planted with rice to obtain a more precise field environment. The differences of N2O fluxes between the rice-planted and non-planted soils might have been caused by the rice plant’s rhizospheric activities, which may have influenced the N2O consumption potential in the rice plants’ rhizosphere. The N2O consumption potential was significantly increased with increasing N fertilization rates and was highly correlated with rice biomass yields. Therefore, the decrease in N2O fluxes by N fertilization in rice-planted soils might have been caused by a decreasing denitrification potential in paddy soils.
Plant Growth Stimulation by Microbial Consortia
Authors: Santoyo Gustavo , Guzmán-Guzmán Paulina , Parra-Cota Fannie Isela , Santos-Villalobos Sergio de los , Orozco-Mosqueda Ma. del Carmen , Glick Bernard R.
Published: January 2021

Plant-associated microorganisms play an important role in agricultural production. Although various studies have shown that single microorganisms can exert beneficial effects on plants, it is increasingly evident that when a microbial consortium—two or more interacting microorganisms—is involved, additive or synergistic results can be expected. This occurs, in part, due to the fact that multiple species can perform a variety of tasks in an ecosystem like the rhizosphere. Therefore, the beneficial mechanisms of plant growth stimulation (i.e., enhanced nutrient availability, phytohormone modulation, biocontrol, biotic and abiotic stress tolerance) exerted by different microbial players within the rhizosphere, such as plant-growth-promoting bacteria (PGPB) and fungi (such as Trichoderma and Mycorrhizae), are reviewed. In addition, their interaction and beneficial activity are highlighted when they act as part of a consortium, mainly as mixtures of different species of PGPB, PGPB–Mycorrhizae, and PGPB–Trichoderma, under normal and diverse stress conditions. Finally, we propose the expansion of the use of different microbial consortia, as well as an increase in research on different mixtures of microorganisms that facilitate the best and most consistent results in the field.
Yield Potential and Variability of Teff (Eragrostis tef (Zucc.) Trotter) Germplasms under Intensive and Conventional Management Conditions
Authors: Bayable Muluken , Tsunekawa Atsushi , Haregeweyn Nigussie , Alemayehu Getachew , Tsuji Wataru , Tsubo Mitsuru , Adgo Enyew , Tassew Asaminew , Ishii Takayoshi , Asaregew Fekremariam , Masunaga Tsugiyuki
Published: January 2021

Teff is the most strategic cereal crop grown from high rainfall to drought prone areas of Ethiopia, where it covers nearly 30% of the land allotted for cereals. However, its productivity remains very low due to lack of knowledge and research interventions. To investigate the grain yield potential, estimate the genetic parameters, and the diversity, a pot experiment with intensive management and a field experiment with conventional management at two contrasting locations for two seasons using the same 317 genotypes and additional 3 improved cultivars in the field experiment were carried out. The results showed highly significant variation among the genotypes for grain yield, biomass, harvest index, and phenological traits under both experiments. The best linear unbiased predictor (BLUP)-adjusted grain yield performance of the genotypes ranged from 4.2 to 8.8 g/plant in the intensive management and 1.8 to 4.3 g/plant in the field growing condition with conventional management. Coefficient of genetic variation, heritability, and expected genetic advance for grain yield were the highest in both experiments. Among the phenological traits, the grain filling period in the intensive growing condition exceptionally showed the highest genetic coefficient of variation and genetic advance. The high grain yield performance and wider range of the harvest index observed under the intensive management condition with moderate to high heritability signifies the genetic potential of teff for further improvement through trait recombination.
Disclosing the Nutritional Quality Diversity of Portuguese Common Beans—The Missing Link for Their Effective Use in Protein Quality Breeding Programs
Authors: Mecha Elsa , Natalello Sofia , Carbas Bruna , da Silva Andreia Bento , Leitão Susana T. , Brites Carla , Veloso Maria Manuela , Rubiales Diego , Costa Judite , Cabral Maria de Fátima , Figueira Maria E. , Vaz Patto Maria C. , Bronze Maria R.
Published: January 2021

The common bean (Phaseolus vulgaris L.) represents a sustainable and affordable source of protein, namely, to populations with vegetarian dietary habits. Despite the national germplasm genetic diversity, little is known about the Portuguese accessions’ nutritional and protein quality, leading to their underuse in breeding programs. To fill this gap, a representative collection (106 accessions) was cropped under two contrasting environments (traditional versus heat stress) and evaluated in terms of nutritional quality by near-infrared spectroscopy. Protein quality was assessed, under the stressful environment, considering the individual amino acid contents and the activity of trypsin inhibitors through mass spectrometry (LC-MS/MS) and spectrophotometry, respectively. On top of strong genotypic control, the nutritional composition (protein, fat, fiber, moisture and ash) was also highly influenced by the environment and by genotype × environment interaction, with a clear nutritional quality ranking change for the accessions in heat stress conditions. Classified into three clusters, the accessions from the cluster with the highest individual amino acid and protein contents also showed higher trypsin inhibitor activity (TIA). Since different levels of TIA had no translation into contrasting protein digestibility, breeders focusing on common beans’ protein quality improvement, especially under challenging warming climate conditions, may take advantage of this group of accessions.
An Overview on Agroecology and Organic Agriculture Strategies for Sustainable Crop Production
Authors: Diacono Mariangela , Trinchera Alessandra , Montemurro Francesco
Published: January 2021

Within the new “European Green Deal”, the European Commission defined crucial challenges for the agriculture of future decades, such as making food production more sustainable by considering the local pedo-climatic and socio-economic specificities [...]
Tracing Mastitis Pathogens—Epidemiological Investigations of a Pseudomonas aeruginosa Mastitis Outbreak in an Austrian Dairy Herd
Authors: Schauer Bernhard , Wald Regina , Urbantke Verena , Loncaric Igor , Baumgartner Martina
Published: January 2021

The present study describes an outbreak of Pseudomonas (P.) aeruginosa mastitis in a 20-cow dairy herd where throughout genotyping of isolates reusable udder towels were identified as the source of infection. Sampling of cows during three herd surveys and bacteriological culturing showed that P. aeruginosa was isolated from nine cows with a total of 13 infected quarters. Mastitis occurred as mild clinical or subclinical infection. P. aeruginosa was additionally isolated from a teat disinfectant solution, containing N-(3-aminopropyl)-N-dodécylpropane-1,3-diamine 1 as active component, and microfiber towels used for pre-milking teat preparation. Disc diffusion antimicrobial resistance testing revealed that all isolates were susceptible to piperacillin, piperacillin-tazobactam, ceftazidime, cefepime, aztreonam, imipenem, meropenem, tobramycin, amikacin, and ciprofloxacin. Thirty-two isolates of milk samples and 22 randomly selected isolates of one udder towel and of the teat disinfectant solution were confirmed as P. aeruginosa with matrix-assisted laser desorption, ionization time-of-flight mass spectrometry (MALDI Tof MS). Isolates were further characterized with rep-PCR and randomly amplified polymorphic DNA (RAPD) as well as with multiple locus variable-number tandem repeat analysis (MLVA). Results obtained in this study suggested that one single strain was responsible for the whole outbreak. The transmission occurred throughout a contaminated teat cleaning solution as a source of infection. The farmer was advised to change udder-preparing routine and to cull infected cows.
Effects of Soy Protein Concentrate in Starter Phase Diet on Growth Performance, Blood Biochemical Indices, Carcass Traits, Immune Organ Indices and Meat Quality of Broilers
Authors: Zhang Qianyun , Zhang Shan , Cong Guanglei , Zhang Yijian , Madsen Marianne Hjøllund , Tan Benjie , Shi Shourong
Published: January 2021

Soybean meal (SBM) is high in antinutritional factors (ANFs), which is not conducive to the starter growth of broilers. The purpose of this study was to investigate the effects of soy protein concentrate (SPC) in starter diet on growth performance, carcass traits, meat quality, immune organ indices and blood biochemical indices of broilers. A total of 384 1-day-old Arbor Acres (AA) male broilers (46.05 ± 0.37 g) with similar body weight were randomly divided into 4 groups with 8 replicates in each group and 12 broilers in each replicate. The experiment was divided into three phases: in starter phase (1–10 d), birds were fed a corn-SBM-based basal mash diet (control) and the basal diet was supplemented with SPC at 4% (SPC4), 8% (SPC8), 12% (SPC12). In the grower phase (11–21 d) and the finisher phase (22–42 d), the birds in all four treatment groups were fed the same diets. The results showed that the body weight was significantly increased in the SPC8 and SPC12 groups of broilers at 10 d and 42 d (p < 0.05). The average daily gain was significantly increased in the SPC12 group of broilers at 1–10 d and 1–42 d (p < 0.05). The average daily feed intake was significantly increased in the SPC8 and SPC12 groups of broilers at 1-10 d (p < 0.05). The feed conversion rates at 1–42 d (p = 0.055) tended to decline in the SPC12 group. The carcass yield and the thymus indices were significantly increased in the SPC12 group of broilers at 42 d (p < 0.05). Alanine aminotransferase (ALT)/aspartate aminotransferase (AST) tended to decline in SPC12 group at 10 d (p = 0.055) and total protein (TP) tended to increase in the SPC12 group at 42 d (p = 0.080). The contents of total cholesterol (T-CHO) and high-density lipoprotein (HDL) were significantly elevated in the SPC12 group of broilers at 42 d (p < 0.05). In conclusion, dietary inclusion of 12% SPC as a starter diet can be recommended due to the positive effects on broilers.
Evaluation of Bone Healing Using Contrast-Enhanced Ultrasonography in Non-Operative Treatment of Tibial Fracture in a Puppy Dog
Authors: Macrì Francesco , Angileri Vito , Russo Teresa , Russo Maria Tomiko , Tabbì Marco , Di Pietro Simona
Published: January 2021

A 10-month-old mixed-breed male dog was presented with an oblique tibial fracture. The dog was treated with a Robert Jones-like bandage as a conservative approach, and was subjected to X-ray, B-mode, Color Doppler and contrast-enhanced ultrasound (CEUS) examinations during the fracture healing, in order to assess bone hemodynamic changes. B-mode, Power Doppler and CEUS examinations of the fracture gap were performed at 7, 20, 35, and 50 days post-trauma. Quantitative analysis of CEUS and perfusion parameters were obtained. On CEUS, a steep incline in signal numbers was visible in fracture gap at 7 days with peaks at 35 days, after which the vascularization decreases gradually over the next days. In this study, CEUS provided important information on the early stages of the callus formation and on the healing of neighboring tissues, allowing recognition of a correct bone healing. Moreover, the number of vascular signals on CEUS was greater than that on Doppler images on the same day. This report showed the application of CEUS in controlling the fracture healing process. CEUS could be a method of monitoring the remedial processes, assessing the tibial fracture perfusion characterized by low-velocity, small-volume blood flows.
A Disorder of Sex Development in a Holstein–Friesian Heifer with a Rare Mosaicism (60,XX/90,XXY): A Genetic, Anatomical, and Histological Study
Authors: Szczerbal Izabela , Komosa Marcin , Nowacka-Woszuk Joanna , Uzar Tomasz , Houszka Marek , Semrau Jerzy , Musial Magdalena , Barczykowski Michal , Lukomska Anna , Switonski Marek
Published: January 2021

In this study, we describe an eighteen-month-old Holstein–Friesian heifer with a deformed vulva, located abdominally. The heifer showed typical signs of estrus. A comprehensive anatomical and histopathological examination revealed a blind-ended vagina and an additional section of urethra, which became a part of the shortened penis. Cytogenetic analysis showed the presence of two cell lines: 60,XX and 90,XXY. The frequency of the triploid cell line was low (3%) in leukocytes and elevated (35%) in fibroblasts. The molecular detection of Y-linked genes (SRY and AMELY) in the blood, skin, hair follicles, and buccal epithelial cells confirmed the presence of a cell line carrying the Y chromosome. Genotyping of 16 microsatellite markers in DNA isolated from hair follicles and fibroblast culture showed the presence of one (homozygous) or two variants (heterozygous) at all the studied loci, and allowed chimerism to be excluded. We concluded that the heifer had diploid/triploid (60,XX/90,XXY) mosaicism. To our knowledge, this is only the fifth such case to be reported worldwide in this species. Since cytogenetic studies are routinely performed on in vitro cultured leukocytes, we suspect that the prevalence of this chromosome abnormality is underestimated, as it is known from published reports that the frequency of the triploid cell line is usually very low in leukocytes.
Head-Only Stunning of Turkeys Part 2: Subjective and Objective Assessment of the Application of AC and DC Waveforms
Authors: Wotton Steve , Grist Andrew , O’Callaghan Mike , van Klink Ed
Published: January 2021

Electrical stunning is likely to remain an important stunning method for turkeys at slaughter. The purpose of this study is to understand the application of various waveforms of alternating current (AC) and pulsed direct currents (DC), head-only, to turkeys and to improve the effectiveness of handheld stunning of turkeys. We evaluated the effectiveness of stunning by documenting physical responses and recording electroencephalograms (EEGs). For the assessment of physical responses, the stunning voltage was varied depending on the proportion of animals effectively stunned at a certain voltage level. If all turkeys in a group of 10 were stunned, the voltage was decreased, and the next group was stunned. This was repeated until not all turkeys showed signs of being effectively stunned. The experiment was then repeated at the voltage level just above the one that showed incomplete effective stunning. The effects of the stunning on the EEG recording was assessed in 16 turkeys to measure the occurrence of epileptiform EEGs, in 14 turkeys to assess epileptiform EEGs after neck-cut (bleeding), and in 14 turkeys to assess the effect of increased voltage and reduced frequency on epileptiform EEGs. Assessing EEGs in a laboratory setting contributes considerably to the understanding of electrical stunning procedures. Voltages between 125 and 250 V, depending on the waveform assessed, were effective in producing an effective stun in turkeys in this study.
A Cat Skeleton from the Balatlar Church Excavation, Sinop, Turkey
Authors: Onar Vedat , Köroğlu Gülgün , Armutak Altan , Öncü Öğül Emre , Siddiq Abu B. , Chrószcz Aleksander
Published: January 2021

In the 2015 excavation season, an east–west oriented burial (2015-Grave-14) built with large dimension stone blocks was unearthed on the south edge of “Area IVi” at the Balatlar Church in Sinop, on the northeastern Black Sea coast of Turkey. In this grave, which is dated between the end of the 6th century AD and the first half of the 7th century AD, a human skeleton was found with the head to the west and a cat skeleton was carefully placed next to the right femur. This study on the burial and the cat skeleton within it shows that, compared to the Roman period, the status of cats reached a higher level during the Byzantine period. It was found that alongside of being a pet, the Balatlar cat was a young healthy female individual that instinctively hunted rodents and birds, given that the remains of a rat and a sparrow were found in the region of the abdominal cavity, corresponding with the stomach location in the living animal. The grave presents the most significant direct archaeological evidence of a pet–human bond recorded at any Byzantine site so far.
Avian Mycobacteriosis and Molecular Identification of Mycobacterium avium Subsp. avium in Racing Pigeons (Columba livia domestica) in Greece
Authors: Tsiouris Vasilios , Kiskinis Konstantinos , Mantzios Tilemachos , Dovas Chrysostomos , Mavromati Natalia , Filiousis Georgios , Brellou Georgia , Vlemmas Ioannis , Georgopoulou Ioanna
Published: January 2021

In this report, cases of avian mycobacteriosis in two lofts of racing pigeons are described. Three racing pigeons of 2-year old from the first loft (A) and four racing pigeons of 4–5 years old from the second loft (B) were submitted to the Unit of Avian Medicine for clinical examination and necropsy. In the case history chronic and debilitating disease was reported. The clinical signs included emaciation, depression, lameness, periorbital swelling and diarrhea, although the appetite was normal. Post mortem lesions involved an enlarged spleen with multiple different sized yellow nodules. Similar lesions were also observed in the liver, conjunctiva of the inferior eyelids and in the femoral bone marrow. The suspicion of avian mycobacteriosis was based on history, clinical signs and typical lesions. In order to confirm the diagnosis, histopathology was performed on tissue sections and revealed the presence of multiple granulomas with central necrosis. In addition, Ziehl-Neelsen positive bacilli were observed in histological sections and smears from the granulomas of the affected tissues. Molecular analysis identified the causative agent as Mycobacterium avium subsp. avium. This is the first case report of avian mycobacteriosis in Greece, which describes the presence of granulomatous conjunctivitis and the molecular identification of M. avium subsp. avium as the causative agent in racing pigeons.
Early Immunocastration of Pigs: From Farming to Meat Quality
Authors: Werner Daniela , Baldinger Lisa , Bussemas Ralf , Büttner Sinje , Weißmann Friedrich , Ciulu Marco , Mörlein Johanna , Mörlein Daniel
Published: January 2021

The study aimed to test a very early immunization of pigs to prevent boar taint with regard to its practicability and influence on production performance, its reliability in ensuring good meat and fat quality, and animal welfare. Immunization was already conducted at piglet production stage and could be easily integrated into routine vaccination (week 3) and weaning practices (week 7). The fattening and slaughter performance of the animals was not affected by the immunization regime and was within the usual range. In addition, there were no abnormalities in animal behavior and the prevalence of injuries caused by aggressive interactions. All animals were classified as infertile on the basis of the histological examination of the testicles. However, the testosterone levels measured at slaughter were significantly higher in animals of the early immunization regime than in animals subjected to the standard immunization regime. Androstenone and skatole levels as the main components of boar taint were, on average, higher and varied to a greater extent in early immunized animals. Furthermore, the comparison of the immunization scheme did not result in significant differences for meat quality and for fatty acid composition.
Oregano (Origanum vulgare) Extract Enhances Zebrafish (Danio rerio) Growth Performance, Serum and Mucus Innate Immune Responses and Resistance against Aeromonas hydrophila Challenge
Authors: Rashidian Ghasem , Boldaji Javad Tahmasebi , Rainis Simona , Prokić Marko D. , Faggio Caterina
Published: January 2021

This study evaluated the dietary effects of an ultrasound-assisted extract of Origanum vulgare on the growth, antioxidant and immune responses (serum and mucosal) and resistance of zebrafish (Danio rerio) against Aeromonas hydrophila. Four hundred and forty adult zebrafish were distributed into 12 tanks and fed 4 experimental diets including 0% (control), 0.5% (M1), 1% (M2) and 2% (M3) of the extract per kg−1 diet for eight weeks. Fish were then challenged with A. hydrophila and mortality was recorded for 10 days. Results revealed that the extract exerted potent effects on growth parameters of weight gain and specific growth rate. The feed conversion ratio was significantly lower in fish fed extract-incorporated diets. O. vulgare extract improved antioxidant and immune responses, resulting in less sensitivity to oxidative stress and a higher survival rate when challenged with A. hydrophila. Overall, the greatest effects were observed in individuals with 1% dietary inclusion of the extract. These results suggest that the extract from the plant Origanum vulgare possesses a great potential to be used in the aquaculture industry and that zebrafish is an appropriate model for nutrition studies.
Factors That Optimize Reproductive Efficiency in Dairy Herds with an Emphasis on Timed Artificial Insemination Programs
Authors: Cardoso Consentini Carlos Eduardo , Wiltbank Milo Charles , Sartori Roberto
Published: January 2021

Reproductive efficiency is closely tied to the profitability of dairy herds, and therefore successful dairy operations seek to achieve high 21-day pregnancy rates in order to reduce the calving interval and days in milk of the herd. There are various factors that impact reproductive performance, including the specific reproductive management program, body condition score loss and nutritional management, genetics of the cows, and the cow comfort provided by the facilities and management programs. To achieve high 21-day pregnancy rates, the service rate and pregnancy per artificial insemination (P/AI) should be increased. Currently, there are adjustments in timed artificial insemination (TAI) protocols and use of presynchronization programs that can increase P/AI, even to the point that fertility is higher with some TAI programs as compared with AI after standing estrus. Implementation of a systematic reproductive management program that utilizes efficient TAI programs with optimized management strategies can produce high reproductive indexes combined with healthy cows having high milk production termed “the high fertility cycle”. The scientific results that underlie these concepts are presented in this manuscript along with how these ideas can be practically implemented to improve reproductive efficiency on commercial dairy operations.
Genetic Features of Extended-Spectrum β-Lactamase-Producing Escherichia coli from Poultry in Mayabeque Province, Cuba
Authors: Baez Michel , Espinosa Ivette , Collaud Alexandra , Miranda Iliana , Montano Damarys de las Nieves , Feria Angel L. , Hernández-Fillor Rosa Elena , Obregón Dasiel , Alfonso Pastor , Perreten Vincent
Published: January 2021

A total of 434 poultry cloacal samples were collected from seven different farms in different years (2013–2015) in the Cuban province of Mayabeque and analyzed for the presence of third-generation cephalosporin-resistant Escherichia coli (3GC-R-Ec). Sixty-two 3GC-R-Ec isolates were recovered in total from the farms, with detection rates of 2.9% in 2013, 10.3% in 2014, and 28.7% in 2015. Characterization of 32 3GC-R-Ec isolates revealed the presence of the extended-spectrum β-lactamase (ESBL) genes blaCTX-M-1 (n = 27), blaCTX-M-15 (n = 4), and blaCTX-M-1 together with blaLAP-2 (n = 1). The isolates also contained different proportions of genes conferring decreased susceptibility to sulfonamides (sul1, sul2, sul3), trimethoprim (dfrA1, dfrA7, dfrA12, dfrA14, dfrA17), tetracyclines (tet(A), tet(B)), aminoglycosides (aac(6′)-Ib-cr, strA, strB), chloramphenicol (cmlA1, floR), macrolides (mph(A), mph(D)), and quinolones (qnrS, qnrB, aac(6′)-Ib-cr) as well as mutations in the fluoroquinolone-resistance determining regions of GyrA (S83L, D87N, D87Y) and ParC (S80I, E84G). The isolates belonged to 23 different sequence types and to phylogroups A (n = 25), B1 (n = 5), and D (n = 2), and they contained plasmid-associated incompatibility groups FII, X1, HI1, HI2, N, FIA, and FIB. These findings reveal a genetically diverse population of multiresistant ESBL-producing E. coli in poultry farms in Cuba, which suggests multiple sources of contamination and the acquisition of antibiotic resistance genes.
Special Issue: Pathogenic Escherichia coli: Infections and Therapies
Authors: Martinez-Medina Margarita
Published: January 2021

Escherichia coli is a facultative anaerobic Gram-negative bacterium from the Enterobacteriaceae family that colonizes the gastrointestinal tract of warm-blooded animals shortly after birth, and it is a lifelong colonizer of adults [...]
Lipid Oxidation and Colour Stability of Lamb and Yearling Meat (Muscle longissimus lumborum) from Sheep Supplemented with Camelina-Based Diets after Short-, Medium-, and Long-Term Storage
Authors: Ponnampalam Eric N. , Butler Kym L. , Muir Stephanie K. , Plozza Tim E. , Kerr Matthew G. , Brown Wayne G. , Jacobs Joe L. , Knight Matthew I.
Published: January 2021

This study investigated the impact of feeding pelleted diets containing camelina (Camelina sativa L. Crantz) hay (CAHP) or camelina meal (CAMP) as a supplement compared with a control pellet (CONP) diet, without vitamin E fortification. The fatty acid profile, retail colour, and lipid oxidative stability of lamb and yearling meat (m. longissimus lumborum) stored for short-, medium-, or long-periods (2 days (fresh), 45 days and 90 days) under chilled to semi-frozen conditions were determined. The CAMP diet altered key fatty acids (p < 0.05) in a nutritionally beneficial manner for human health compared to the other diets, with increased total omega-3, decreased omega-6 fatty acids and decreased omega-6/omega-3 ratio of muscle. Muscle vitamin E concentration was lower (p < 0.05) for both camelina diets (CAMP and CAHP) when compared with the CONP diet, with the average concentrations less than 1 mg/kg muscle for all three treatments. Animal type and storage length were factors that all affected (p < 0.05) colour and lipid oxidative stability of meat. These results emphasise the importance of vitamin E concentration in meat stored for extended periods under semi-frozen conditions to maintain desirable meat colour during retail display, and to avoid off-flavour development of the cooked meat.
Activation of Nrf2 by Electrophiles Is Largely Independent of the Selenium Status of HepG2 Cells
Authors: Tauber Sarah , Sieckmann Maria Katharina , Erler Katrin , Stahl Wilhelm , Klotz Lars-Oliver , Steinbrenner Holger
Published: January 2021

Selenoenzymes, whose activity depends on adequate selenium (Se) supply, and phase II enzymes, encoded by target genes of nuclear factor erythroid 2-related factor 2 (Nrf2), take part in governing cellular redox homeostasis. Their interplay is still not entirely understood. Here, we exposed HepG2 hepatoma cells cultured under Se-deficient, Se-adequate, or Se-supranutritional conditions to the Nrf2 activators sulforaphane, cardamonin, or diethyl maleate. Nrf2 protein levels and intracellular localization were determined by immunoblotting, and mRNA levels of Nrf2 target genes and selenoproteins were assessed by qRT-PCR. Exposure to electrophiles resulted in rapid induction of Nrf2 and its enrichment in the nucleus, independent of the cellular Se status. All three electrophilic compounds caused an enhanced expression of Nrf2 target genes, although with differences regarding extent and time course of their induction. Whereas Se status did not significantly affect mRNA levels of the Nrf2 target genes, gene expression of selenoproteins with a low position in the cellular “selenoprotein hierarchy”, such as glutathione peroxidase 1 (GPX1) or selenoprotein W (SELENOW), was elevated under Se-supplemented conditions, as compared to cells held in Se-deficient media. In conclusion, no major effect of Se status on Nrf2 signalling was observed in HepG2 cells.
Possible Beneficial Effects of N-Acetylcysteine for Treatment of Triple-Negative Breast Cancer
Authors: Kwon Youngjoo
Published: January 2021

N-acetylcysteine (NAC) is a widely used antioxidant with therapeutic potential. However, the cancer-promoting effect of NAC observed in some preclinical studies has raised concerns regarding its clinical use. Reactive oxygen species (ROS) can mediate signaling that results in both cancer-promoting and cancer-suppressing effects. The beneficial effect of NAC may depend on whether the type of cancer relies on ROS signaling for its survival and metastasis. Triple-negative breast cancer (TNBC) has aggressive phenotypes and is currently treated with standard chemotherapy as the main systemic treatment option. Particularly, basal-like TNBC cells characterized by inactivated BRCA1 and mutated TP53 produce high ROS levels and rely on ROS signaling for their survival and malignant progression. In addition, the high ROS levels in TNBC cells can mediate the interplay between cancer cells and the tissue microenvironment (TME) to trigger the recruitment and conversion of stromal cells and induce hypoxic responses, thus leading to the creation of cancer-supportive TMEs and increased cancer aggressiveness. However, NAC treatment effectively reduces the ROS production and ROS-mediated signaling that contribute to cell survival, metastasis, and drug resistance in TNBC cells. Therefore, the inclusion of NAC in standard chemotherapy could probably provide additional benefits for TNBC patients.
Mechanical and Physical Characterization of Papercrete as New Eco-Friendly Construction Material
Authors: Cardinale Tiziana , D’Amato Michele , Sulla Roselena , Cardinale Nicola
Published: January 2021

The manufacturing of Portland cement is responsible for a big amount of energy and greenhouse gas (GHG) emission. Therefore, to date, it is imperative to find alternative materials to replace a major part of cement for sustainable concrete constructions. The present study forms a part of an on-going research project on the application of new cementitious matrices produced using different types of recycled materials. In particular, it focuses on the use of pulp and waste paper to partially replace Portland cement at varying percentages for producing a new lightweight mortar, frequently named papercrete. The development of this economical and eco-friendly material may permit of recycling a big amount of waste paper leading to lower housing costs with also ecological benefits. To this scope, an experimental campaign in the laboratory is carried out to characterize this new innovative material from a physical and mechanical point of view. The preliminary results of this on-going experimental campaign are illustrated and commented on in this paper. The obtained results confirm the possibility of applying this partially-recycled material as a possible alternative for strengthening existing panels of masonry.
Bridge-Mediated RET between Two Chiral Molecules
Authors: Salam A
Published: January 2021

Molecular quantum electrodynamics (QED) theory is employed to calculate the rate of resonance energy transfer (RET) between a donor, D, described by an electric dipole and quadrupole, and magnetic dipole coupling, and an identical acceptor molecule, A, that is mediated by a third body, T, which is otherwise inert. A single virtual photon propagates between D and T, and between T and A. Time-dependent perturbation theory is used to compute the matrix element, from which the transfer rate is evaluated using the Fermi golden rule. This extends previous studies that were limited to the electric dipole approximation only and admits the possibility of the exchange of excitation between a chiral emitter and absorber. Rate terms are computed for specific pure and mixed multipole-dependent contributions of D and A for both an oriented arrangement of the three particles and for the freely tumbling situation. Mixed multipole moment contributions, such as those involving electric–magnetic dipole or electric dipole–quadrupole coupling at one center, do not survive random orientational averaging. Interestingly, the mixed electric–magnetic dipole D and A rate term is non-vanishing and discriminatory, exhibiting a dependence on the chirality of the emitter and absorber, and is entirely retarded. It vanishes, however, if D and A are oriented perpendicularly to one another. Near- and far-zone asymptotes of isotropic contributions to the rate are also evaluated, demonstrating radiationless short-range transfer and inverse-square radiative exchange at very large separations.
The Spatial Distribution and Influencing Factors of Employment Multipliers in China’s Expanding Cities
Authors: Huang Daquan , He Han , Liu Tao
Published: January 2021

In the process of urbanization in developing countries, creating enough jobs to realize the transition from an agricultural population to a non-agricultural population is a major goal of development. The differences and localities of cities need to be considered in the policymaking process. This study estimated the local employment multipliers of expanding cities in China and calculated the employment multiplier of each city. First, there are obvious differences in the size of employment multipliers across cities; therefore, it is necessary to adopt different policies in employment promotion. Second, an inverted-U-shape relationship is detected between employment multiplier and city size, namely the larger the city, the greater the employment multiplier, but when the city size exceeds a certain value, the employment multiplier begins to decline. Third, different degrees of influence are generated by factors for cities at different levels of economic development. Based on the research results, we suggest that expansion of the trade sector be promoted in small- and medium-sized cities, to give full play to its employment multiplier effect; meanwhile, in large cities, the degree of specialization of the trade sector and diversification of the non-trade sector should be improved.
Smoothed Particle Hydrodynamics Simulation of Orthogonal Cutting with Enhanced Thermal Modeling
Authors: Afrasiabi Mohamadreza , Klippel Hagen , Roethlin Matthias , Wegener Konrad
Published: January 2021

Smoothed Particle Hydrodynamics (SPH) is a mesh-free numerical method that can simulate metal cutting problems efficiently. The thermal modeling of such processes with SPH, nevertheless, is not straightforward. The difficulty is rooted in the computationally demanding procedures regarding convergence properties and boundary treatments, both known as SPH Grand Challenges. This paper, therefore, intends to rectify these issues in SPH cutting models by proposing two improvements: (1) Implementing a higher-order Laplacian formulation to solve the heat equation more accurately. (2) Introducing a more realistic thermal boundary condition using a robust surface detection algorithm. We employ the proposed framework to simulate an orthogonal cutting process and validate the numerical results against the available experimental measurements.
A Typology Model of Temporary Facility Constraints for Automated Construction Site Layout Planning
Authors: Kim Minguk , Ryu Han-Guk , Kim Tae Wan
Published: January 2021

This study sought to develop a typology model of temporary facility constraints that can be used practically in construction site layout planning (CSLP) automation models. A triangulated methodology (literature review, in-depth interviews, and actual case studies) was used to identify constraints of 11 temporary facilities that are considered mainly in CSLP and to classify them into six constraint types (i.e., dimensional, regional, relocation, non-overlap, inter-facility distance, and visibility constraints) and seven subtypes. In addition, this study proposed computational modeling methods that would allow a computer to judge whether or not the constraints are met by a created construction site layout. This study contributes to CSLP theory by providing a typology model of temporary facilities that can be used in the model-based verification of the created construction site layout and constraint processing condition in CSLP optimization models. This would also help provide efficient, safe, and eco-friendly construction site management, while refraining from experience- and intuition-centered CSLP practices.
Comparison of Trabecular Bone Mineral Density Measurement Using Hounsfield Unit and Trabecular Microstructure in Orthodontic Patients Using Cone-Beam Computed Tomography
Authors: Kim Tae-Hyun , Lee Dong-Yul , Jung Seok-Ki
Published: January 2021

The aim of this study was to measure the bone mineral density of specific regions of maxilla, mandible, and first cervical vertebra using the Hounsfield unit and trabecular microstructure pattern analysis and to compare the two methods. In this study, cone-beam computed tomography (CBCT) images were obtained from 58 patients. Trabecular thickness, trabecular number, trabecular separation, and bone volume fraction were measured in 484 regions for trabecular microstructure parameters and Hounsfield unit was measured for the grayscale value. There was no difference in bone mineral density between the right and left side in every site and between males and females. Trabecular thickness and trabecular number were high in the order of anterior base of the maxilla, mandibular body, first cervical vertebra, and mandibular condyle. Bone volume fraction and Hounsfield unit were high in the order of anterior base of the maxilla, mandibular body, mandibular condyle, and first cervical vertebra (p < 0.05). Trabecular thickness, trabecular number, and bone volume fraction was positively correlated to the Hounsfield unit, and trabecular separation was negatively correlated to the Hounsfield unit (p < 0.005). This study suggests that it is possible to compare the bone mineral density of trabecular bone in various sites using the Hounsfield unit and trabecular microstructure pattern analysis.
Investigation of Regenerative Braking Performance of Brushless Direct Current Machine Drive System
Authors: Kivanc Omer Cihan , Ustun Ozgur
Published: January 2021

The brushless direct current (BLDC) machines which are preferred in light electric vehicles (LEVs) come forward as high regenerative braking capability machines due to their permanent magnet excitation and relatively simple operation. In this paper, the regenerative braking capability limits of BLDC machines and their drive circuits are examined by taking into account nonlinear circuit parameters and battery internal resistance variation. During energy recovery from mechanical port to electrical port, the inverter of BLDC machine is operated as a boost converter which enables power flow to a battery. However, the regeneration performance is also heavily dependant on the battery condition, particularly the temperature. By means of the developed detailed circuit model including the non-ideal effects of the boosting converter and the increase of the internal resistance variation which is caused by the temperature variation of the battery and ambient temperature, the specific duty cycle can be determined. The specific duty ratio is then applied in a proposed approach for various operation scenarios. The experimental tests are implemented by a 400 W BLDC machine drive system controlled via a TMS320F28335 digital signal processor. The experimental results show that the proposed comprehensive model presents a proper performance estimation of regenerative braking system under varying battery temperature.
Effect of Pitch Parameters on Aerodynamic Forces of a Straight-Bladed Vertical Axis Wind Turbine with Inclined Pitch Axes
Authors: Guo Jia , Qu Timing , Lei Liping
Published: January 2021

Pitch regulation plays a significant role in improving power performance and achieving output control in wind turbines. The present study focuses on a novel, pitch-regulated vertical axis wind turbine (VAWT) with inclined pitch axes. The effect of two pitch parameters (the fold angle and the incline angle) on the instantaneous aerodynamic forces and overall performance of a straight-bladed VAWT under a tip-speed ratio of 4 is investigated using an actuator line model, achieved in ANSYS Fluent software and validated by previous experimental results. The results demonstrate that the fold angle has an apparent influence on the angles of attack and forces of the blades, as well as the power output of the wind turbine. It is helpful to further study the dynamic pitch regulation and adaptable passive pitch regulation of VAWTs. Incline angles away from 90° lead to the asymmetric distribution of aerodynamic forces along the blade span, which results in an expected reduction of loads on the main shaft and the tower of VAWTs.
Effect of Fly Ash on Compressive Strength, Drying Shrinkage, and Carbonation Depth of Mortar with Ferronickel-Slag Powder
Authors: Choi Se-Jin , Kim Ji-Hwan , Bae Sung-Ho , Oh Tae-Gue
Published: January 2021

In recent years, efforts to reduce greenhouse gas emissions have continued worldwide. In the construction industry, a large amount of CO2 is generated during the production of Portland cement, and various studies are being conducted to reduce the amount of cement and enable the use of cement substitutes. Ferronickel slag is a by-product generated by melting materials such as nickel ore and bituminous coal, which are used as raw materials to produce ferronickel at high temperatures. In this study, we investigated the fluidity, microhydration heat, compressive strength, drying shrinkage, and carbonation characteristics of a ternary cement mortar including ferronickel-slag powder and fly ash. According to the test results, the microhydration heat of the FA20FN00 sample was slightly higher than that of the FA00FN20 sample. The 28-day compressive strength of the FA20FN00 mix was approximately 39.6 MPa, which was higher than that of the other samples, whereas the compressive strength of the FA05FN15 mix including 15% of ferronickel-slag powder was approximately 11.6% lower than that of the FA20FN00 mix. The drying shrinkage of the FA20FN00 sample without ferronickel-slag powder was the highest after 56 days, whereas the FA00FN20 sample without fly ash showed the lowest shrinkage compared to the other mixes.
Backlight Unit Excessive Dimming Based on Perceptual Image Analysis
Authors: Yang Seungwoo , Shin Hyungsik , You JaeHee
Published: January 2021

A method to maximize backlight dimming of liquid crystal displays (LCDs) based on human visual system are proposed to minimize power consumption of display panels. Based on images, the proposed method optimizes global, local, and red, green, blue (RGB) backlight dimming by enhancing dimming about 12% on average for various images while maintaining tolerable degradation of perceived image qualities in pixel saturation areas. The method considers and utilizes the brightness sensitivity and contrast response functions of human visual system using the mean luminance and the contrast of an image, which are mathematically modelled to allow optimization for display panels and application areas. A simulator that can calculate various dimming cases with the evaluations of numerical and perceptual image qualities as well as power consumption amount is introduced. With pattern and real photo images, the degree of power savings and the preservation of image quality of the proposed method are verified to outperform conventional approaches with high scores of the mean opinion score (MOS) and the structural similarity index measure (SSIM) over 0.97 while saving more than 10% of power dissipation.
Hybrid Techniques to Predict Solar Radiation Using Support Vector Machine and Search Optimization Algorithms: A Review
Authors: Álvarez-Alvarado José Manuel , Ríos-Moreno José Gabriel , Obregón-Biosca Saul Antonio , Ronquillo-Lomelí Guillermo , Ventura-Ramos Eusebio , Trejo-Perea Mario
Published: January 2021

The use of intelligent algorithms for global solar prediction is an ideal tool for research focused on the use of solar energy. Forecasting solar radiation supports different applications focused on the generation and transport of energy in places where there are no meteorological stations. Different solar radiation prediction techniques have been applied in different time horizons, such as neural networks (ANN) or machine learning (ML), with the latter being the most important. The support vector machine (SVM) is a classification method of the ML that is used to predict solar radiation. To obtain a better accuracy of prediction data, search optimization algorithms (SOA) such as genetic algorithms (GA) and the particle swarm optimization algorithm (PSO) were used to optimize the prediction accuracy by searching the model parameters. This article presents a review of different hybrid SVM models with SOA applied to obtain the best parameters to reduce the prediction error of solar radiation using meteorological variables. Research articles from the last 5 years on solar radiation prediction using SVM models and hybrid SMV optimized models with SOA were studied. The results show that SVM with GA presents a better performance than the classical SVM models using the Radial basis kernel function for prediction parameters.