bluuebunny/crossref_metadata_2025 huggingface dataset:
New Perspectives in Sustainable Aquaculture
Authors: Longo Caterina , Pierri Cataldo
Published: July 2023

The strong growth of the aquaculture industry can represent a serious threat to aquatic ecosystems and requires the development of new strategies to control and minimize the impact of such activities on the structure and function of biological communities [...]
Improved Sugarcane-Based Fermentation Processes by an Industrial Fuel-Ethanol Yeast Strain
Authors: Muller Gabriela , de Godoy Victor R. , Dário Marcelo G. , Duval Eduarda H. , Alves-Jr Sergio L. , Bücker Augusto , Rosa Carlos A. , Dunn Barbara , Sherlock Gavin , Stambuk Boris U.
Published: July 2023

In Brazil, sucrose-rich broths (cane juice and/or molasses) are used to produce billions of liters of both fuel ethanol and cachaça per year using selected Saccharomyces cerevisiae industrial strains. Considering the important role of feedstock (sugar) prices in the overall process economics, to improve sucrose fermentation the genetic characteristics of a group of eight fuel-ethanol and five cachaça industrial yeasts that tend to dominate the fermentors during the production season were determined by array comparative genomic hybridization. The widespread presence of genes encoding invertase at multiple telomeres has been shown to be a common feature of both baker’s and distillers’ yeast strains, and is postulated to be an adaptation to sucrose-rich broths. Our results show that only two strains (one fuel-ethanol and one cachaça yeast) have amplification of genes encoding invertase, with high specific activity. The other industrial yeast strains had a single locus (SUC2) in their genome, with different patterns of invertase activity. These results indicate that invertase activity probably does not limit sucrose fermentation during fuel-ethanol and cachaça production by these industrial strains. Using this knowledge, we changed the mode of sucrose metabolism of an industrial strain by avoiding extracellular invertase activity, overexpressing the intracellular invertase, and increasing its transport through the AGT1 permease. This approach allowed the direct consumption of the disaccharide by the cells, without releasing glucose or fructose into the medium, and a 11% higher ethanol production from sucrose by the modified industrial yeast, when compared to its parental strain.
Sporotrichosis in Older Adults: A Cohort Study of 911 Patients from a Hyperendemic Area of Zoonotic Transmission in Rio de Janeiro, Brazil
Authors: Gomes Rachel da Silva Ribeiro , do Valle Antonio Carlos Francesconi , Freitas Dayvison Francis Saraiva , de Macedo Priscila Marques , Oliveira Raquel de Vasconcellos Carvalhaes , Almeida-Paes Rodrigo , Zancopé-Oliveira Rosely Maria , Gutierrez-Galhardo Maria Clara
Published: July 2023

Generally, older people tend to suffer from more severe infections than younger adults. In addition, there are accumulations of comorbidities and immune senescence in some cases. This cohort study evaluated the clinical and epidemiological characteristics of older adults (≥60 years old) with sporotrichosis. The cohort consisted of 911 patients with a median age of 67 years, most of whom were female (72.6%), white (62.1%), and afflicted with comorbidities (64.5%). The lymphocutaneous form occurred in 62% of the patients, followed by the fixed form (25.7%), cutaneous disseminated form (8.9%), and extracutaneous/disseminated forms (3.3%). In this study, we draw attention to the frequency of osteoarticular involvement (2.1%) secondary to skin lesions such as osteomyelitis and/or tenosynovitis. A clinical cure was achieved in 87.3% of cases. Itraconazole was used in 81.1% of cases, while terbinafine was used in 22.7% of cases, usually in low doses. Survival analysis showed that the median treatment time was 119 days, and the multiple Cox model demonstrated that the presentation of a black coloration and diabetes was associated with a longer treatment time required to establish a cure. Therefore, these subgroups should be monitored more closely to reduce possible difficulties during treatment. It would be interesting to conduct more studies analyzing older adults with sporotrichosis from different geographic areas to better comprehend the disease in this group.
Kidney Allograft Monitoring by Combining Donor-Derived Cell-Free DNA and Molecular Gene Expression: A Clinical Management Perspective
Authors: Rizvi Asim , Faiz Sara , Thakkar Parin H. , Hussain Syed , Gamilla-Crudo Ann N. , Kueht Michael , Mujtaba Muhammad A.
Published: July 2023

Donor-derived cell-free DNA (dd-cfDNA) may safely assess kidney allograft rejection. Molecular Microscope (MMDx®) gene expression may offer increased precision to histology. This single-center retrospective study monitored kidney transplant recipients for rejection at specified time intervals by utilizing creatinine (SCr), proteinuria, donor-specific antibodies (DSAs), and dd-cfDNA. A clinically indicated biopsy sample was sent for histopathology and MMDx®. Patients were categorized into rejection (Rej) and non-rejection (NRej) groups, and further grouped according to antibody-mediated rejection (ABMR) subtypes. Rej and NRej groups included 52 and 37 biopsies, respectively. Median follow-up duration was 506 days. DSAs were positive in 53% and 22% of patients in both groups, respectively (p = 0.01). Among these groups, pre- and post-intervention median SCr, proteinuria, and dd-cfDNA at 1 month, 2 months, and at the last follow-up revealed significant difference for dd-cfDNA (all p = 0.01), however, no difference was found for SCr and proteinuria (p > 0.05). The AUC was 0.80 (95% CI: 0.69–0.91), with an optimal dd-cfDNA criterion of 2.2%. Compared to histology, MMDx® was more likely to diagnose ABMR (79% vs. 100%) with either C4d positivity or negativity and/or DSA positivity or negativity. Hence, a pre- and post-intervention allograft monitoring protocol in combination with dd-cfDNA, MMDx®, and histology has aided in early diagnosis and timely individualized intervention.
Causes of Non-Adherence to Allergen-Specific Immunotherapy: A Foundation towards a Patient-Personalized Approach
Authors: Ridolo Erminia , Nicoletta Francesca , Barone Alessandro , Ottoni Martina , Senna Gianenrico , Canonica Giorgio Walter
Published: July 2023

Background. Allergen-specific immunotherapy (AIT) is the only disease-modifying therapy for allergic conditions, resulting in a long-lasting tolerance beyond the duration of the treatment. Due to the strong relationship between the effectiveness, its optimal duration (at least three years) and the observation of the correct administration protocol, appropriate adherence to the plan of treatment represents a critical factor for the therapeutical success of AIT. Methods. Analysis of studies about the rate of adherence in subcutaneous and sublingual immunotherapy, which are the main routes of administration of AIT. Results. There are different causes leading to a premature interruption of the therapy or to it being incorrectly carried out; the most reported include erroneous expectations of the effectiveness and the adverse effects, economic issues, inconvenience and unrelated clinical conditions. Conclusions. An attentive analysis of the main causes of dropouts may be useful to improve the management of these patients and to develop new strategies for a personalized approach. These strategies should be dynamic, involving attentive communication between the physician and the patient about all the possible criticalities, especially in the initial phase of the therapy, and facilitating, as much as possible, access to healthcare providers over the course of the maintenance phase, including by exploiting technological tools.
Preemptive TPMT Genotyping and Adherence to Genotype-Based Therapeutic Recommendations Reduces the Healthcare Cost in Patients Receiving Azathioprine or 6-Mercaptopurine for Autoimmune Diseases
Authors: Valdez-Acosta Sarahí , Zubiaur Pablo , Casado Miguel Angel , Novalbos Jesús , Casajús Ana , Campodónico Diana , Oyagüez Itziar , Abad-Santos Francisco
Published: July 2023

A cost analysis of thiopurine treatment was carried out in 257 patients, with 153 preemptively genotyped for TPMT and 104 retrospectively genotyped in a Spanish setting. The healthcare cost was significantly higher in patients retrospectively genotyped compared to those who were preemptively genotyped (p < 0.001). TPMT intermediate metabolizers (IMs) (n = 23) showed a 3.3-fold higher healthcare cost when compared to normal metabolizers (NMs) (p < 0.001). The healthcare cost in patients with a TPMT IM phenotype whose physician adhered to the genotype-informed recommendation was similar than the cost in TPMT NMs and was significantly lower than IMs whose physician did not adhere to the therapeutic recommendation (3.8-fold, p = 0.016). Myelotoxicity occurrence was significantly lower in patients preemptively vs. retrospectively genotyped (2.0% and 21.2%, respectively, p < 0.001). Patients who developed myelotoxicity showed a significantly higher healthcare cost than those who did not (4.10-fold, p < 0.001). Overall, 87% of patients whose dose was not adjusted despite being TPMT IMs suffered myelotoxicity, while only one of the eight patients (13%) whose dose was adjusted suffered myelotoxicity (p < 0.001). In conclusion, TPMT preemptive genotyping and physician adherence to genotype-informed therapeutic recommendations prevents myelotoxicity and significantly reduces the healthcare cost, and it is therefore essential for the sustainability of the Spanish healthcare system.
Risk Factors of Suboptimal Coronary Blood Flow after a Percutaneous Coronary Intervention in Patients with Acute Anterior Wall Myocardial Infarction
Authors: Maruszak Natalia , Pilch Weronika , Januszek Rafał , Malinowski Krzysztof Piotr , Surdacki Andrzej , Chyrchel Michał
Published: July 2023

Background and aims: Primary percutaneous coronary intervention (PCI) is regarded as the most preferred strategy in ST-segment elevation myocardial infarction (STEMI). Thrombolysis in Myocardial Infarction (TIMI) flow grade has been an important and cohesive predictor of outcomes in STEMI patients. We sought to evaluate potential variables associated with the risk of suboptimal TIMI flow after PCI in patients with anterior wall STEMI. Methods: We evaluated 107 patients admitted to our hospital between 1 January 2019 and 31 December 2021 with a diagnosis of anterior wall STEMI treated with primary PCI. Results: Suboptimal TIMI flow grade (≤2) after PCI occurred in 14 (13%) patients while grade 3 was found in 93 (87%) of them presenting with anterior wall STEMI. Failure to achieve optimal TIMI 3 flow grade after PCI was associated with lower TIMI grade prior to PCI (OR 0.5477, 95% CI 0.2589–0.9324, p = 0.02), greater troponin concentration before (OR 1.0001, 95% CI 1–1.0001, p = 0.0028) and after PCI (OR 1.0001, 95% CI 1–1.0001, p = 0.0452) as well as lower mean minimal systolic blood pressure (OR 0.9653, 95% CI 0.9271–0.9985, p = 0.04). Conclusions: Among predictors of suboptimal TIMI flow grade after PCI, we noted lower TIMI grade flow pre-PCI, greater serum troponin concentrations in the periprocedural period and lower mean minimal systolic blood pressure.
The Olive Grove Landscape as a Tourist Resource in Andalucía: Oleotourism
Authors: Dancausa Millán Mª Genoveva , Sanchez-Rivas García Javier , Millán Vázquez de la Torre Mª Genoveva
Published: July 2023

Andalucía, located in the southern region of Spain, is the world’s largest producer of olive oil. It is home to over 70 million olive trees, which shape the distinctive landscape of the olive groves typical of the Mediterranean Basin. This research focuses on analyzing the olive grove landscape and the rich culture associated with the olive tree as a tourist activity known as oleotourism. This form of tourism would attract an increased number of tourists and generate funds for the preservation of the olive tree heritage if UNESCO declared it a World Heritage Site, similar to other cultural landscapes. Furthermore, it would help diversify the region’s tourism industry, which has traditionally focused on sun and beach tourism. This study is a continuation of previous research initiated by the authors in 2017. It is observed that the profile of oleotourists before and after the COVID-19 pandemic has not varied significantly, although there has been an increase in the number of people engaging in this type of tourism, as evidenced by the results obtained with the SARIMA model. The SARIMA model is used for forecasting and analyzing time series data. These findings indicate that investing in this tourism activity would be profitable for local residents, making it a sustainable form of tourism that is compatible with the environment and the local community.
Analysis of Spatio-Temporal Pattern Changes and Driving Forces of Xinjiang Plain Oases Based on Geodetector
Authors: Zhang Ji , Zhang Pei , Gu Xinchen , Deng Mingjiang , Lai Xiaoying , Long Aihua , Deng Xiaoya
Published: July 2023

Water and soil resources are the key elements required to maintain the stability and sustainable development of oases in inland drylands. This study takes oases in Xinjiang as an example, and reveals the spatial and temporal distribution characteristics of oases across Xinjiang, as well as the dynamic migration and transformation process of oases and the mutual influence of human activities on oasis resources, through a combination of land movement and attitude indicators, use of a gravity model, and Geodetector. The results show that: (1) the area containing the oases in the study has expanded, increasing by 24.0% between 1990 and 2020; (2) both artificial oases (AO) and natural oases (NO) were tilted in a northeast–southwest direction, with the center point of AOs moving slightly to the northeast, and the centripetal force and dispersion degree did not change significantly; the center point of NO moved significantly to the northeast, and the dispersion degree weakened, showing spatial convergence; (3) cultivated land and grassland were the main land types of oasis evolution, and their contribution to evolution was over 70%; and (4) the Geodetector results show that the three main factors driving the evolution of oases were vegetation cover, population density, and GDP. The research results have practical implications for land-use management planning and ecological environmental protection in arid zones.
Rethinking Industrial Heritage Tourism Resources in the EU: A Spatial Perspective
Authors: Yan Mi , Li Qingmiao , Zhang Jiazhen
Published: July 2023

Industrial heritage serves as a poignant testament to the historical narrative of industrial civilization. The European Route of Industrial Heritage (ERIH) is a pan-European network that connects industrial sites and museums to enhance public awareness of the significance of the industrial heritage sector and to promote sustainable development practices in urban areas through collaboration and cooperation between these sites. The ERIH is crucial in promoting economic, cultural, and social values associated with industrial heritage and creating opportunities for tourism and education in Europe. Taking the ERIH as the research object, the nearest-neighbor index, kernel density, geographic detector, and other methods are used in this study to explore the spatial distribution characteristics and influencing factors of European industrial heritage. The results of this study have implications for urban and regional planning endeavors aimed at advancing sustainable urban development. Furthermore, they contribute to cultivating a sense of place and identity by identifying and preserving industrial heritage institutions while fostering social cohesion and community identity practices. The results show that (1) the spatial distribution of European industrial heritage presents a cohesive distribution, and the spatial distribution is uneven; (2) the spatial distribution of European industrial heritage forms “dual cores, dual centers, one belt, three zones and multiple scattered points”—the center of gravity shows a trend of “first north and then east” and the north–south movement is relatively small; and (3) a combination of natural and social factors shape the spatial distribution of industrial heritage. Natural conditions, such as altitude, topography, and hydrological characteristics, influence the distribution patterns of industrial heritage sites. Meanwhile, human factors, including infrastructure level, cultural tourism potential, and social development, play a pivotal role in determining spatial distribution patterns. Among these factors, the socio-economic level exhibits the strongest influence, with an explanatory power of 0.763. The results of this study can contribute to the conservation and tourism practices regarding industrial heritage sites, thus promoting sustainable urban development practices.
Satisfaction with Sustainable Tourism—A Case of the Special Nature Reserve “Meadows of Great Bustard”, Vojvodina Province
Authors: Trišić Igor , Stanić Jovanović Sara , Štetić Snežana , Nechita Florin , Candrea Adina Nicoleta
Published: July 2023

The Special Nature Reserve, a part of the nature “Meadows of Great Bustard” (MGB), is a protected area with an extremely rare and sensitive ecosystem. The MGB spreads in the northeast of Vojvodina in Serbia. The natural potentials of MGB, in addition to diverse flora and fauna, are symbolized by a jeopardized species of bird called the Great Bustard (Otis tarda). Only a few specimens of this species live in this protected area, and they need to be protected. Globally, the Great Bustard is vulnerable species (VU) and a seriously protected species in Serbia. The goal of the research in this paper is to determine whether sustainable tourism affects the satisfaction of residents, using a quantitative method through the Prism of Sustainability (PoS) research model, which includes the technique of surveying respondents (residents). In this part of Vojvodina lives a population that has specific social characteristics. The cultural legacy, customs of this part of Banat, way of treating nature, local crafts, and local events stand out among those characteristics. The favorable geographical position of MGB, the proximity of Romania and Hungary, and good traffic connections with numerous cities in Serbia and the region represent significant potential for tourism development. The natural motives of MGB and ecological interests should be a priority in planning and developing tourism. A nature-based tourism destination can be formed by combining natural and social motives. The main forms of tourism, important for the development of this area, are ecotourism, scientific research tourism, and birdwatching tourism in the MGB. The significant results of the research are those that point out that the respondents rated ecological and socio-cultural sustainability as the most important. Also, these two dimensions have the greatest impact on sustainable tourism in the MGB. After the conducted research, it can be concluded that the residents are satisfied with sustainable tourism, i.e., sustainable tourism significantly affects the satisfaction of residents.
SE-YOLOv7 Landslide Detection Algorithm Based on Attention Mechanism and Improved Loss Function
Authors: Liu Qing , Wu Tingting , Deng Yahong , Liu Zhiheng
Published: July 2023

With the continuous development of computer vision technology, more and more landslide identification detection tasks have started to shift from manual visual interpretation to automatic computer identification, and automatic landslide detection methods based on remote sensing satellite images and deep learning have been gradually developed. However, most existing algorithms often have the problem of low precision and weak generalization in landslide detection. Based on the Google Earth Engine platform, this study selected landslide image data from 24 study areas in China and established the DN landslide sample dataset, which contains a total of 1440 landslide samples. The original YOLOv7 algorithm model was improved and optimized by applying the SE squeezed attention mechanism and VariFocal loss function to construct the SE-YOLOv7 model to realize the automatic detection of landslides in remote sensing images. The experimental results show that the mAP, Precision value, Recall value, and F1-Score of the improved SE-YOLOv7 model for landslide identification are 91.15%, 93.35%, 94.54%, and 93.94%, respectively. At the same time, through a field investigation and verification study in Qianyang County, Baoji City, Shaanxi Province, comparing the detection results of SE-YOLOv7, it is concluded that the improved SE-YOLOv7 can locate the landslide location more accurately, detect the landslide range more accurately, and have fewer missed detections. The research results show that the algorithm model has strong detection accuracy for many types of landslide image data, which provides a technical reference for future research on landslide detection based on remote sensing images.
Improving Public Action to Mitigate River Flooding in Niamey (Niger)
Authors: Mahamane Saidou Oumarou , Oumarou Amadou , Piñeira Mantiñán María José
Published: July 2023

The purpose of this paper is to analyze the effects of repeated floods in the city of Niamey and the strategies developed by the state in terms of prevention, rehabilitation, and care for victims. Although numerous framework documents have been drafted in the last decade and urbanization in flood zones has been limited, the reality in Niamey shows that urban sprawl is increasing along the riverbank and the population facing vulnerable situations is growing. The inefficiency of state action determines that, on the one hand, it is non-governmental institutions that need to develop improvement plans—with the recovery of the city being dependent on the arrival of their funds and their distribution in those areas that donor countries consider most suitable—and on the other hand, it is the citizens—already vulnerable—who must find their own resilience mechanisms to try to survive the next flood. This study reveals that this diversity of players is involved in flood management through emergency relief, solidarity in rehousing, and providing support for living and non-living flood victims. Despite this mobilization, the actions undertaken are hampered by a number of constraints. Insufficient financial and human resources, a lack of foresight on the part of the authorities, and poorly coordinated actions are the main difficulties limiting the success of public action in the face of flooding. Through a qualitative approach, combining documentary research, direct observation and semi-structured interviews, we analyze the mobilization of actors around the management of the effects of floods in Niamey.
Unexpected Adverse Events of Immune Checkpoint Inhibitors
Authors: Shalata Walid , Yakobson Alexander , Cohen Aharon Y. , Goldstein Iris , Saleh Omar Abu , Dudnik Yulia , Rouvinov Keren
Published: July 2023

The introduction of immune checkpoint inhibitors (ICIs) has revolutionized cancer treatment standards and significantly enhanced patient prognoses. However, the utilization of these groundbreaking therapies has led to the observation and reporting of various types of adverse events, commonly known as immune-related adverse events (irAEs). In the following article, we present four patients who encountered uncommon toxicities induced by ICIs. The first patient was a 59-year-old female diagnosed with stage 4 lung adenocarcinoma. She received immunotherapy (pembrolizumab) together with chemotherapy and subsequently developed autonomic neuropathy (AN). The next two patients also received chemo-immunotherapy (pembrolizumab) and were both 63-year-old males with stage 4 lung adenocarcinoma. One of the two experienced palmoplantar keratoderma, while the other presented with Reiter’s syndrome (urethritis, conjunctivitis and arthritis). The 4th patient, an 80-year-old male with stage 4 squamous cell carcinoma of the lung, received chemo-immunotherapy (pembrolizumab) and developed myasthenia gravis.
GC-ToF-MS Profiling and In Vitro Inhibitory Effects of Selected South African Plants against Important Mycotoxigenic Phytopathogens
Authors: Mariri Ntagi Gerald , Dikhoba Preachers Madimetja , Mongalo Nkoana Ishmael , Makhafola Tshepiso Jan
Published: July 2023

The harmful effects following the ingestion of mycotoxin-contaminated food include the induction of cancers, mutagenicity, immune suppression, and toxicities that target organs of the digestive, cardiovascular, and central nervous systems. Synthetic fungicides are generally associated with a high toxic residue in food and the development of excessive fungal resistance. This study aimed to determine the antifungal activities against mycotoxigenic fungi of selected South African plant leaves and potentially develop plant-derived bio-fungicides, and, furthermore, to explore the in vitro antioxidant activity and the phytochemical spectra of the compounds of the selected medicinal plant extracts. The extracts were tested for antifungal activity against phytopathogenic strains using a microdilution broth assay. Bauhinia galpinii extracts exhibited the lowest minimum inhibitory concentration (MIC) against C. cladospoides and P. haloterans at 24 h incubation periods. C. caffrum had good antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) with 50% inhibitory concentration (IC50) values of 0.013 mg/mL while B. galpini had IC50 values of 0.053 against free radicals of 2,2′-azinobis (3-ethylbenzthiazoline-6-suphonic acid (ABTS). The antimycotoxigenic and antioxidant activity exerted by both B. galpinii and C. caffrum may well be attributed to high TPC. In the GC-ToF-MS analysis, all the selected medicinal plants exhibited the presence of Hexadecanoic acid at varying % areas, while both B. galpinii and C. caffum exhibited the presence of lupeol at % area 2.99 and 3.96, respectively. The compounds identified, particularly the ones with higher % area, may well explain the biological activity observed. Although the selected medicinal plants exhibited a notable biological activity, there is a need to explore the safety profiles of these plants, both in vitro and in vivo.
Pathogenesis of Alcoholic Fatty Liver a Narrative Review
Authors: Seitz Helmut K. , Moreira Bernardo , Neuman Manuela G.
Published: July 2023

Alcohol effect hepatic lipid metabolism through various mechanisms, leading synergistically to an accumulation of fatty acids (FA) and triglycerides. Obesity, as well as dietary fat (saturated fatty acids (FA) versus poly-unsaturated fatty acids (PUFA)) may modulate the hepatic fat. Alcohol inhibits adenosine monophosphate activated kinase (AMPK). AMPK activates peroxisome proliferator activated receptor a (PPARα) and leads to a decreased activation of sterol regulatory element binding protein 1c (SRABP1c). The inhibition of AMPK, and thus of PPARα, results in an inhibition of FA oxidation. This ß-oxidation is further reduced due to mitochondrial damage induced through cytochrome P4502E1 (CYP2E1)-driven oxidative stress. Furthermore, the synthesis of FAs is stimulated through an activation of SHREP1. In addition, alcohol consumption leads to a reduced production of adiponectin in adipocytes due to oxidative stress and to an increased mobilization of FAs from adipose tissue and from the gut as chylomicrons. On the other side, the secretion of FAs via very-low-density lipoproteins (VLDL) from the liver is inhibited by alcohol. Alcohol also affects signal pathways such as early growth response 1 (Egr-1) associated with the expression of tumour necrosis factor α (TNF α), and the mammalian target of rapamycin (mTOR) a key regulator of autophagy. Both have influence the pathogenesis of alcoholic fatty liver. Alcohol-induced gut dysbiosis contributes to the severity of ALD by increasing the metabolism of ethanol in the gut and promoting intestinal dysfunction. Moreover, pathogen-associated molecular patterns (PAMPS) via specific Toll-like receptor (TLR) bacterial overgrowth leads to the translocation of bacteria. Endotoxins and toxic ethanol metabolites enter the enterohepatic circulation, reaching the liver and inducing the activation of the nuclear factor kappa-B (NFκB) pathway. Pro-inflammatory cytokines released in the process contribute to inflammation and fibrosis. In addition, cellular apoptosis is inhibited in favour of necrosis.
The Progression in Developing Genomic Resources for Crop Improvement
Authors: Ruperao Pradeep , Rangan Parimalan , Shah Trushar , Thakur Vivek , Kalia Sanjay , Mayes Sean , Rathore Abhishek
Published: July 2023

Sequencing technologies have rapidly evolved over the past two decades, and new technologies are being continually developed and commercialized. The emerging sequencing technologies target generating more data with fewer inputs and at lower costs. This has also translated to an increase in the number and type of corresponding applications in genomics besides enhanced computational capacities (both hardware and software). Alongside the evolving DNA sequencing landscape, bioinformatics research teams have also evolved to accommodate the increasingly demanding techniques used to combine and interpret data, leading to many researchers moving from the lab to the computer. The rich history of DNA sequencing has paved the way for new insights and the development of new analysis methods. Understanding and learning from past technologies can help with the progress of future applications. This review focuses on the evolution of sequencing technologies, their significant enabling role in generating plant genome assemblies and downstream applications, and the parallel development of bioinformatics tools and skills, filling the gap in data analysis techniques.
Novel Anti-Inflammatory Therapies in Coronary Artery Disease and Acute Coronary Syndromes
Authors: Dimitroglou Yannis , Aggeli Constantina , Theofilis Panagiotis , Tsioufis Panagiotis , Oikonomou Evangelos , Chasikidis Christos , Tsioufis Konstantinos , Tousoulis Dimitris
Published: July 2023

Evidence suggests that inflammation plays an important role in atherosclerosis and the consequent clinical presentation, including stable coronary artery disease (CAD) and acute coronary syndromes (ACS). The most essential elements are cytokines, proteins with hormone-like properties that are produced by the immune cells, endothelial cells, platelets, fibroblasts, and some stromal cells. Interleukins (IL-1β and IL-6), chemokines, interferon-γ (IFN-γ), and tumor necrosis factor-alpha (TNF-α) are the cytokines commonly associated with endothelial dysfunction, vascular inflammation, and atherosclerosis. These molecules can be targeted by commonly used therapeutic substances or selective molecules that exert targeted anti-inflammatory actions. The most significant anti-inflammatory therapies are aspirin, statins, colchicine, IL-1β inhibitors, and IL-6 inhibitors, along with novel therapies such as TNF-α inhibitors and IL-1 receptor antagonists. Aspirin and statins are well-established therapies for atherosclerosis and CAD and their pleiotropic and anti-inflammatory actions contribute to their efficacy and favorable profile. Colchicine may also be considered in high-risk patients if recurrent ACS episodes occur when on optimal medical therapy according to the most recent guidelines. Recent randomized studies have also shown that therapies specifically targeting inflammatory interleukins and inflammation can reduce the risk for cardiovascular events, but these therapies are yet to be fully implemented in clinical practice. Preclinical research is also intense, targeting various inflammatory mediators that are believed to be implicated in CAD, namely repeated transfers of the soluble mutant of IFN-γ receptors, NLRP3 inflammasome inhibitors, IL-10 delivery by nanocarriers, chemokine modulatory treatments, and reacting oxygen species (ROS) targeting nanoparticles. Such approaches, although intriguing and promising, ought to be tested in clinical settings before safe conclusions can be drawn. Although the link between inflammation and atherosclerosis is significant, further studies are needed in order to elucidate this association and improve outcomes in patients with CAD.
Study on the Influence of Micro-Features in the Surface Topography of the Slider Raceway on the Dynamic Friction Factor of the Guide Rail Pair
Authors: Kang Mingxia , Hua Dezheng , Guo Xiaoqiang
Published: July 2023

During the operation of the guide rail pair, the accuracy of the guide rail pair is decreased by friction and wear, and the overall performance of the machine tool is directly affected. The influence of surface micro-features of the slider raceway on the dynamic friction factor of the guide rail pair is analyzed in this paper. The relationship between the geometric characteristics of the surface micro-features and the dynamic friction factor is studied, and a prediction model of the dynamic friction factor is established. Meanwhile, the correctness and rationality of the predicted model is verified by the orthogonal experiments of the friction force. It is found that the dynamic friction factor increases with the increase of the groove depth, the height of the peaks and peak–valleys on the surface of the slider raceway. The above research provides a basis for the study of the friction and wear characteristics of guide rail pairs and lays a foundation for improving its friction and wear performance.
Microstructural, Mechanical, and Electrochemical Characterization of CrMoNbTiZr High-Entropy Alloy for Biomedical Application
Authors: Akinwekomi Akeem , Akhtar Farid
Published: July 2023

High-entropy alloys (HEA) with superior biocompatibility, high pitting resistance, minimal debris accumulation, and reduced release of metallic ions into surrounding tissues are potential replacements for traditional metallic bio-implants. A novel equiatomic HEA based on biocompatible metals, CrMoNbTiZr, was consolidated by spark plasma sintering (SPS). The relative sintered density of the alloy was about 97% of the theoretical density, indicating the suitability of the SPS technique to produce relatively dense material. The microstructure of the sintered HEA consisted of a BCC matrix and Laves phase, corresponding to the prediction of the thermodynamic CALPHAD simulation. The HEA exhibited a global Vickers microhardness of 531.5 ± 99.7 HV, while the individual BCC and Laves phases had hardness values of 364.6 ± 99.4 and 641.8 ± 63.0 HV, respectively. Its ultimate compressive and compressive yield strengths were 1235.7 ± 42.8 MPa and 1110.8 ± 78.6 MPa, respectively. The elasticity modulus of 34.9 ± 2.9 GPa of the HEA alloy was well within the range of cortical bone and significantly lower than the values reported for commonly used biomaterials made from Ti-based and Cr–Co-based alloys. In addition, the alloy exhibited good resistance to bio-corrosion in PBS and Hanks solutions. The CrMoNbTiZr HEA exhibited an average COF of 0.43 ± 0.06, characterized mainly by abrasive and adhesive wear mechanisms. The CrMoNbTiZr alloy’s mechanical, bio-corrosion, and wear resistance properties developed in this study showed a good propensity for application as a biomaterial.
The Preparation, Characterization, and Pressure-Influenced Dihydrogen Interactions of Tetramethylphosphonium Borohydride
Authors: Jaroń Tomasz
Published: July 2023

Tetramethylphosphonium borohydride was synthesized via an ion metathesis reaction in a weakly-coordinating aprotic environment. [(CH3)4P]BH4, in contrast to related [(CH3)4N]+ compounds which tend to crystallize in a tetragonal system, adopts the distorted wurtzite structure (P63mc), resembling some salts containing analogous ions of As and Sb. [(CH3)4P]BH4 decomposes thermally in several endo- and exothermic steps above ca. 240 °C. This renders it more stable than [(CH3)4N]BH4, with a lowered temperature of decomposition onset by ca. 20 °C and solely exothermic processes observed. Raman spectra measured at the 0–10 GPa range indicate that a polymorphic transition occurs within 0.53–1.86 GPa, which is further confirmed by the periodic DFT calculations. The latter suggests a phase transition around 0.8 GPa to a high-pressure phase of [(CH3)4N]BH4. The P63mc phase seems to be destabilized under high pressure by relatively closer dihydrogen interactions, including the C–H…H–C contacts.
Chemical Pressure Effect on the Stabilization of Rock-Salt ZnO—Lin−2MeOn−1 Solid Solutions Synthesized at High Pressure
Authors: Sokolov Petr S. , Baranov Andrey N. , Solozhenko Vladimir L.
Published: July 2023

Metastable ZnO—Lin−2MeOn−1 (Me = Sc3+, Ti4+, Ta5+) solid solutions with a rock-salt structure were synthesized through the solid-state reaction of ZnO with Lin−2Men+On−1 (n = 3, 4, 5) complex oxides at 7.7 GPa and 1300–1500 K. In all investigated systems, single-phase rock-salt solid solutions can be quenched down to ambient conditions in a wide (up to 80 mol% ZnO) concentration range. The phase composition, thermal stability, and thermal expansion of the recovered rock-salt solid solutions were studied by synchrotron powder X-ray diffraction. At ambient pressure, these solid solutions exhibit high thermal stability (up to 1000 K), with the decomposition temperature and decomposition products depending on the nature of the multiple charge cations.
Review of Waterproof Breathable Membranes: Preparation, Performance and Applications in the Textile Field
Authors: Chang Yawen , Liu Fujuan
Published: July 2023

Waterproof breathable membranes (WBMs) characterized by a specific internal structure, allowing air and water vapor to be transferred from one side to the other while preventing liquid water penetration, have attracted much attention from researchers. WBMs combine lamination and other technologies with textile materials to form waterproof breathable fabrics, which play a key role in outdoor sports clothing, medical clothing, military clothing, etc. Herein, a systematic overview of the recent progress of WBMs is provided, including the principles of waterproofness and breathability, common preparation methods and the applications of WBMs. Discussion starts with the waterproof and breathable mechanisms of two different membranes: hydrophilic non-porous membranes and hydrophobic microporous membranes. Then evaluation criteria and common preparation methods for WBMs are presented. In addition, treatment processes that promote water vapor transmission and prominent applications in the textile field are comprehensively analyzed. Finally, the challenges and future perspectives of WBMs are also explored.
A Photoluminescence Study of Eu3+, Tb3+, Ce3+ Emission in Doped Crystals of Strontium-Barium Fluoride Borate Solid Solution Ba4−xSr3+x(BO3)4−yF2+3y (BSBF)
Authors: Bekker Tatyana B. , Ryadun Alexey A. , Rashchenko Sergey V. , Davydov Alexey V. , Baykalova Elena B. , Solntsev Vladimir P.
Published: July 2023

The present study is aimed at unveiling the luminescence potential of Ba4−xSr3+x(BO3)4−yF2+3y (BSBF) crystals doped with Eu3+, Tb3+, and Ce3+. Owing to the incongruent melting character of the phase, the NaF compound was used as a solvent for BSBF crystal growth. The structure of BSBF: Eu3+ with Eu2O3 concentration of about 0.7(3) wt% was solved in the non-centrosymmetric point group P63mc. The presence of Eu2O3 in BSBF: Eu3+ leads to a shift of the absorption edge from 225 nm to 320 nm. The photoluminescence properties of the BSBF: Ce3+, BSBF: Tb3+, BSBF: Eu3+, and BSBF: Eu3+, Tb3+, Ce3+ crystals have been studied. The unusual feature of europium emission in BSBF is the intensively manifested 5D0→7F0 transition at about 574 nm, which is the strongest for BSBF: Eu3+ at 370 nm excitation and for BSBF: Eu3+, Tb3+, Ce3+ at 300 nm and 370 nm excitations. No evidence of Tb3+→Eu3+ energy transfer was found for BSBF: Eu3+, Tb3+, Ce3+. The PL spectra of BSBF: Eu3+ at 77 and 300 K are similar with CIE chromaticity coordinates of (0.617; 0.378) at 300 nm excitation and (0.634; 0.359) at 395 nm excitation and low correlated color temperature which implies application prospects in the field of lightning. Due to the high intensity of 5D0→7F0 Eu3+ transition at 370 nm excitation, the BSBF: Eu3+ emission is yellow-shifted.
Nanoparticles in Cancer Diagnosis and Treatment
Authors: Baranwal Jaya , Barse Brajesh , Di Petrillo Amalia , Gatto Gianluca , Pilia Luca , Kumar Amit
Published: July 2023

The use of tailored medication delivery in cancer treatment has the potential to increase efficacy while decreasing unfavourable side effects. For researchers looking to improve clinical outcomes, chemotherapy for cancer continues to be the most challenging topic. Cancer is one of the worst illnesses despite the limits of current cancer therapies. New anticancer medications are therefore required to treat cancer. Nanotechnology has revolutionized medical research with new and improved materials for biomedical applications, with a particular focus on therapy and diagnostics. In cancer research, the application of metal nanoparticles as substitute chemotherapy drugs is growing. Metals exhibit inherent or surface-induced anticancer properties, making metallic nanoparticles extremely useful. The development of metal nanoparticles is proceeding rapidly and in many directions, offering alternative therapeutic strategies and improving outcomes for many cancer treatments. This review aimed to present the most commonly used nanoparticles for cancer applications.
A Simplified Method for Predicting Bond–Slip Behaviour of Ribbed Bars and Threaded Rods Glued in Glulam along the Grain
Authors: Peng Jun , Wang Dan-Dan , Kang Shao-Bo , Zhou Shu-Rong
Published: July 2023

The bond between a steel reinforcement/rod and glulam plays a crucial role in the resistance and deformation capacity of timbers joints. Existing studies provide different bond–slip models for reinforcements and rods with different anchorage lengths, in which the relationship between local bond stress and global bond behaviour cannot not be established. This study presents a unified analytical method for predicting the bond–slip behaviour of ribbed bars and threaded rods along the grain using a local bond–slip model of reinforcement at the elastic and post-yield stages. In the analytical method, equilibrium, compatibility, and constitutive models for reinforcement and rods are considered. The method is verified using test data of rebars and rods with different anchorage lengths. Comparisons between the experimental and calculated results suggest that the analytical method yields reasonably good predictions of the load–slip relationship and failure mode. Furthermore, the embedment lengths required for yield and the ultimate strengths of the reinforcement and rods along the grain are determined by assuming uniform bond stress distributions over the elastic and post-yield steel segment. The average bond stress over the entire anchorage length is calculated and compared with existing equations. Design recommendations for anchorage lengths are proposed for ribbed bars and threaded rods glued in glulam.
Development of Molecular Dynamics and Research Progress in the Study of Slag
Authors: Zhou Chaogang , Li Jinyue , Wang Shuhuan , Zhao Jingjing , Ai Liqun , Chen Qinggong , Chen Qiya , Zhao Dingguo
Published: July 2023

Molecular dynamics is a method of studying microstructure and properties by calculating and simulating the movement and interaction of molecules. The molecular dynamics simulation method has become an important method for studying the structural and dynamic characteristics of slag systems and can make up for the shortcomings of existing detection methods and experiments. Firstly, this paper analyzes the development process and application fields of molecular dynamics, summarizes the general simulation steps and software algorithms of molecular dynamics simulation methods, and discusses the advantages and disadvantages of the algorithms and the common functions of the software. Secondly, the research status and application progress of molecular dynamics simulation methods in the study of phosphate, silicate, aluminate and aluminosilicate are introduced. On this basis, a method of combining molecular dynamics simulation with laboratory experiments is proposed, which will help obtain more accurate simulation results. This review provides theoretical guidance and a technical framework for the effective analysis of the microstructure of different slag systems via molecular dynamics, so as to finally meet the needs of iron and steel enterprises in producing high-quality steel grades.
Effect of Potassium Doping on the Structural and Catalytic Properties of Co/MnOx Catalyst in the Steam Reforming of Ethanol
Authors: Greluk Magdalena , Rotko Marek , Słowik Grzegorz , Turczyniak-Surdacka Sylwia , Grzybek Gabriela , Tyszczuk-Rotko Katarzyna
Published: July 2023

The promotional effect of potassium (~1.25 wt%) on a Co/MnOx catalyst was studied for samples prepared by the impregnation method in the steam reforming of ethanol (SRE) process at 420 °C for a H2O/EtOH molar ratio of 12/1. The catalysts were characterized using physicochemical methods to study their textural, structural, and redox properties. The XRD studies revealed that, during the treatment of both cobalt-based catalysts under a hydrogen atmosphere at 500 °C, Co0 and MnO phases were formed by the reduction in Co3O4 and Mn2O3/Mn3O4 phases, respectively. Potassium doping significantly improved stability and ability for the C–C bond cleavage of the Co/MnOx catalyst. The enhancement of activity (at ~25%) and selectivity to hydrogen (at ca. 10%) and the C1 product, mainly carbon dioxide (at ~20%), of the Co/MnOx catalyst upon potassium doping was clarified by the alkali promoter’s impact on the reducibility of the cobalt and manganese oxides. The microscopic observations revealed that fibrous carbon deposits are present on the surface of Co/MnOx and KCo/MnOx catalysts after the SRE reaction and their formation is the main reason these catalysts deactivate under SRE conditions. However, carbon accumulation on the surface of the potassium-promoted catalyst was ca. 12% lower after 18 h of SRE reaction compared to the unpromoted sample.
Current Harmonics Minimization of Permanent Magnet Synchronous Machine Based on Iterative Learning Control and Neural Networks
Authors: Mai Annette , Liu Xinjun , Wagner Bernhard , Hofmann Maximilian
Published: July 2023

Electrical machines generate unwanted flux and current harmonics. Harmonics can be suppressed using various methods. In this paper, the harmonics are significantly reduced using Iterative Learning Control (ILC) and Neural Networks (NNs). The ILC can compensate for the harmonics well for operation at constant speed and current reference values. The NNs are trained with the data from the ILC and help to suppress the harmonics well even in transient operation. The simulation model is based on flux and torque maps, depending on dq-currents and the electrical angle. The maps are generated from FEM simulation of an interior permanent magnet synchronous machine (IPM) and are published with the paper. They are intended to serve other researchers for direct comparison with their own methods. Simulation results in this paper verify that by using ILC and NNs together, current harmonics in transient operation can be eliminated better than without NNs.