bluuebunny/crossref_metadata_2025 huggingface dataset:
Augmented Endothelin Vasoconstriction in Intermittent Hypoxia-Induced Hypertension
Authors: Allahdadi Kyan J. , Walker Benjimen R. , Kanagy Nancy L.
Published: March 2005

We reported previously that simulating sleep apnea in rats by exposing them 7 hours per day to intermittent hypoxia/hypercapnia (IH) elevates plasma endothelin-1 and causes hypertension, which is reversed by an endothelin-1 antagonist. We hypothesized that in this model of sleep apnea–induced hypertension, vascular sensitivity to endothelin-1 is increased in combination with the elevated plasma endothelin-1 to cause the endothelin-1–dependent hypertension. In small mesenteric arteries with endothelial function disabled by passing air through the lumen, diameter and vessel wall [Ca
2+
] were recorded simultaneously. IH arteries demonstrated increased constrictor sensitivity to endothelin-1 (percentage max constriction 100±0% IH versus 80±10% Sham;
P
<0.05). This was accompanied by increased calcium sensitivity of IH arteries. In contrast, constrictor sensitivity and increases in vessel wall [Ca
2+
] to KCl and phenylephrine were not different between IH and Sham arteries. We have shown previously that endothelin-1 constriction in mesenteric arteries is mediated by endothelin A receptors. In the current study, the selective increase in endothelin-1 constriction in IH resistance arteries was accompanied by increased expression of endothelin A receptor expression (densitometry units 271±23 IH versus 158±25 Sham;
P
<0.05). Thus, IH hypertension appears to cause alterations in signaling components unique to endothelin-1 at the receptor level and in postreceptor signaling that increases calcium sensitivity during endothelin A activation. Future studies will determine the specific changes in vascular smooth muscle signaling in IH hypertension causing this augmented contractile phenotype.
Multiple Quantitative Trait Loci for Blood Pressure Interacting Epistatically and Additively on Dahl Rat Chromosome 2
Authors: Dutil Julie , Eliopoulos Vasiliki , Tremblay Johanne , Hamet Pavel , Charron Sophie , Deng Alan Y.
Published: March 2005

Our previous work demonstrated 2 quantitative trait loci (QTLs), C2QTL1 and C2QTL2, for blood pressure (BP) located on chromosome (Chr) 2 of Dahl salt-sensitive (DSS) rats. However, for a lack of markers, the 2 congenic strains delineating C2QTL1 and C2QTL2 could not be separated. The position of the C2QTL1 was only inferred by comparing 2 congenic strains, one having and another lacking a BP effect. Furthermore, it was not known how adjacent QTLs would interact with one another on Chr 2. In the current investigation, first, a critical chromosome marker was developed to separate 2 C2QTLs. Second, a congenic substrain was created to cover a chromosome fragment thought to harbor C2QTL1. Finally, a series of congenic strains was produced to systematically and comprehensively cover the entire Chr 2 segment containing C2QTL2 and other regions previously untested. Consequently, a total of 3 QTLs were discovered, with C2QTL3 located between C2QTL1 and C2QTL2. C2QTL1, C2QTL2, and C2QTL3 reside in chromosome segments of 5.7 centiMorgan (cM), 3.5 cM, and 1.5 cM, respectively. C2QTL1 interacted epistatically with either C2QTL2 or C2QTL3, whereas C2QTL2 and C2QTL3 showed additive effects to each other. These results suggest that BP QTLs closely linked in a segment interact epistatically and additively to one another on Chr 2.
Components of the Metabolic Syndrome and Carotid Atherosclerosis
Authors: Irace Concetta , Cortese Claudio , Fiaschi Elio , Carallo Claudio , Sesti Giorgio , Farinaro Eduardo , Gnasso Agostino
Published: March 2005

Elevated blood pressure is among the factors that contribute to the metabolic syndrome (MetS). It is not known whether subjects with MetS and elevated blood pressure are at the same cardiovascular risk as subjects with MetS but without elevated blood pressure. To clarify this point, we have evaluated the prevalence of carotid atherosclerosis in subjects with MetS with or without elevated blood pressure. A large population was examined (842 women and 1011 men). Blood pressure, lipids, glucose, and waist were measured by routine methods. Carotid atherosclerosis was evaluated by echo Doppler examination. The prevalence of MetS was 24.4% in women and 28.7% in men. The prevalence of carotid atherosclerosis was 35.1% in women and 37.3% in men (
p
=NS), and increased with increasing number of MetS components. Age, smoking, and systolic blood pressure (SBP) were associated with the presence of carotid atherosclerosis (logistic model), whereas age, high-density lipoprotein cholesterol, and SBP were associated with the extent of atherosclerosis (linear model). When comparing subjects with an equal number of MetS components, the prevalence of carotid atherosclerosis was significantly higher in subjects with elevated blood pressure than in those without. No difference in carotid atherosclerosis prevalence was found in subjects bearing or not bearing components of the syndrome other than elevated blood pressure. The present findings demonstrate that subjects with MetS and elevated blood pressure have increased carotid atherosclerosis compared with subjects with MetS but without elevated blood pressure. The diagnosis of MetS per se might not adequately identify subjects at elevated cardiovascular risk.
Annual Cycle of Southeast Asia—Maritime Continent Rainfall and the Asymmetric Monsoon Transition
Authors: Chang C-P. , Wang Zhuo , McBride John , Liu Ching-Hwang
Published: March 2005

In general, the Bay of Bengal, Indochina Peninsula, and Philippines are in the Asian summer monsoon regime while the Maritime Continent experiences a wet monsoon during boreal winter and a dry season during boreal summer. However, the complex distribution of land, sea, and terrain results in significant local variations of the annual cycle. This work uses historical station rainfall data to classify the annual cycles of rainfall over land areas, the TRMM rainfall measurements to identify the monsoon regimes of the four seasons in all of Southeast Asia, and the QuikSCAT winds to study the causes of the variations.

The annual cycle is dominated largely by interactions between the complex terrain and a simple annual reversal of the surface monsoonal winds throughout all monsoon regions from the Indian Ocean to the South China Sea and the equatorial western Pacific. The semiannual cycle is comparable in magnitude to the annual cycle over parts of the equatorial landmasses, but only a very small region reflects the twice-yearly crossing of the sun. Most of the semiannual cycle appears to be due to the influence of both the summer and the winter monsoon in the western part of the Maritime Continent where the annual cycle maximum occurs in fall. Analysis of the TRMM data reveals a structure whereby the boreal summer and winter monsoon rainfall regimes intertwine across the equator and both are strongly affected by the wind–terrain interaction. In particular, the boreal winter regime extends far northward along the eastern flanks of the major island groups and landmasses.

A hypothesis is presented to explain the asymmetric seasonal march in which the maximum convection follows a gradual southeastward progression path from the Asian summer monsoon to the Asian winter monsoon but experiences a sudden transition in the reverse. The hypothesis is based on the redistribution of mass between land and ocean areas during spring and fall that results from different land–ocean thermal memories. This mass redistribution between the two transition seasons produces sea level patterns leading to asymmetric wind–terrain interactions throughout the region, and a low-level divergence asymmetry in the region that promotes the southward march of maximum convection during boreal fall but opposes the northward march during boreal spring.
North Atlantic Decadal Variability: Air–Sea Coupling, Oceanic Memory, and Potential Northern Hemisphere Resonance*
Authors: Wu Lixin , Liu Zhengyu
Published: March 2005

In this paper, the causes and mechanisms of North Atlantic decadal variability are explored in a series of coupled ocean–atmosphere simulations. The model captures the major features of the observed North Atlantic decadal variability. The North Atlantic SST anomalies in the model control simulation exhibit a prominent decadal cycle of 12–16 yr, and a coherent propagation from the western subtropical Atlantic to the subpolar region. A series of additional modeling experiments are conducted in which the air–sea coupling is systematically modified in order to evaluate the importance of air–sea coupling for the North Atlantic decadal variability being studied. This shall be referred to as “modeling surgery.” The results suggest the critical role of ocean–atmosphere coupling in sustaining the North Atlantic decadal oscillation at selected time scales. The coupling in the North Atlantic is characterized by a robust North Atlantic Oscillation (NAO)-like atmospheric response to the SST tripole anomaly, which tends to intensify the SST anomaly and, meanwhile, also provide a delayed negative feedback. This delayed negative feedback is predominantly associated with the adjustment of the subtropical gyre in response to the anomalous wind stress curl in the subtropical Atlantic. Atmospheric stochastic forcing can drive SST patterns similar to those in the fully coupled ocean–atmosphere system, but fails to generate any preferred decadal time scales. The simulated North Atlantic decadal variability, therefore, can be viewed as a coupled ocean–atmosphere mode under the influence of stochastic forcing.

This modeling study also suggests some potential resonance between the Pacific and the North Atlantic decadal fluctuations mediated by the atmosphere. The modeling surgery indicates that the Pacific climate, although not a necessary precondition, can impact the North Atlantic climate variability substantially.
Impact of Indian Ocean Sea Surface Temperature on Developing El Niño*
Authors: Annamalai H. , Xie S. P. , McCreary J. P. , Murtugudde R.
Published: March 2005

Prior to the 1976–77 climate shift (1950–76), sea surface temperature (SST) anomalies in the tropical Indian Ocean consisted of a basinwide warming during boreal fall of the developing phase of most El Niños, whereas after the shift (1977–99) they had an east–west asymmetry—a consequence of El Niño being associated with the Indian Ocean Dipole/Zonal mode. In this study, the possible impact of these contrasting SST patterns on the ongoing El Niño is investigated, using atmospheric reanalysis products and solutions to both an atmospheric general circulation model (AGCM) and a simple atmospheric model (LBM), with the latter used to identify basic processes. Specifically, analyses of reanalysis products during the El Niño onset indicate that after the climate shift a low-level anticyclone over the South China Sea was shifted into the Bay of Bengal and that equatorial westerly anomalies in the Pacific Ocean were considerably stronger. The present study focuses on determining influence of Indian Ocean SST on these changes.

A suite of AGCM experiments, each consisting of a 10-member ensemble, is carried out to assess the relative importance of remote (Pacific) versus local (Indian Ocean) SST anomalies in determining precipitation anomalies over the equatorial Indian Ocean. Solutions indicate that both local and remote SST anomalies are necessary for realistic simulations, with convection in the tropical west Pacific and the subsequent development of the South China Sea anticyclone being particularly sensitive to Indian Ocean SST anomalies. Prior to the climate shift, the basinwide Indian Ocean SST anomalies generate an atmospheric Kelvin wave associated with easterly flow over the equatorial west-central Pacific, thereby weakening the westerly anomalies associated with the developing El Niño. In contrast, after the shift, the east–west contrast in Indian Ocean SST anomalies does not generate a significant Kelvin wave response, and there is little effect on the El Niño–induced westerlies. The Linear Baroclinic Model (LBM) solutions confirm the AGCM’s results.
A Study of the Impact of Off-Equatorial Warm Pool SST Anomalies on ENSO Cycles
Authors: Solomon Amy , Jin Fei-Fei
Published: March 2005

Concurrent with most large El Niño events, cold sea surface temperature (SST) anomalies are observed over the western Pacific warm pool region (WPWP). Observational evidence that SST anomalies that form in the off-equatorial western Pacific during El Niño–Southern Oscillation (ENSO) cycles are forced by subsurface ocean processes equatorward of 12°N and air–sea fluxes poleward of 12°N is presented. It is demonstrated that diurnal mixing in the ocean equatorward of 12°N plays a significant role in bringing subsurface temperature anomalies to the sea surface during an El Niño event.

The role of SST anomalies equatorward of 12°N in ENSO cycles is tested in the Zebiak–Cane coupled model, modified to allow for the impact of subsurface temperatures on SSTs. This coupled model successfully simulates cold SST anomalies in the off-equatorial northwestern Pacific that are observed to occur during the warm phase of ENSO and the atmospheric response to these anomalies, which is composed of both westerlies in the central Pacific and easterlies in the far western equatorial Pacific. It is found that there is little net change in the zonal mean wind stress at the equator, suggesting that the westerlies cancel the impact of the easterlies on the basin-scale tilt of the equatorial zonal mean thermocline depth. The anomalous westerly winds in the central equatorial Pacific are found to increase the amplitude of an El Niño event directly by increasing anomalous warm zonal advection and reducing upwelling. Moreover, the off-equatorial anticyclonic wind stress associated with the cold SST anomalies during the warm phase of ENSO tends to reduce the discharge of the equatorial heat content. Thus, the coupled processes over the western Pacific warm pool can serve as a positive feedback to amplify ENSO cycles.
The Future of Marketing Scholarship: Recruiting for Marketing Doctoral Programs
Authors: Davis Donna F. , McCarthy Teresa M.
Published: March 2005

As demand for business education is rising, the production of business doctorates continues to fall. Between 1995 and 2001, new business doctorates declined 18%, dropping to the lowest point since 1987. In the same time frame, new marketing doctorates dropped by 32%. This article reports the results of a study designed to (1) assess enrollment trends and recruiting practices for marketing doctoral programs, (2) examine decision-making processes of prospective marketing doctoral students, and (3) develop a set of recommendations for improving recruiting in marketing doctoral programs. Results of surveys of marketing doctoral program coordinators, marketing Ph.D. students, and MBA students are reported.
The Motivational Effects of the Classroom Environment in Facilitating Self-Regulated Learning
Authors: Young Mark R.
Published: March 2005

Students can be proactive and engaged or, alternatively, lack initiative and responsibility for their learning. Self-regulated learning involves learning strategies and mental processes that learners deliberately engage to help themselves learn and perform better academically. The results of this study provide empirical support for the theoretical relationships among cognitive evaluation theory, achievement goal theory, and self-regulated learning strategies in the context of the classroom. Superficial learning strategies were linked to extrinsic motivation, while intrinsic motivation determined deep cognitive and metacognitive strategy usage. Perceived autonomy, perceived competence, and task mastery orientation mediated the classroom environment’s effect on intrinsic motivation. These findings suggest that active applicationoriented experience delivered by enthusiastic faculty, who provide high interaction, supportive feedback, and clear goals that emphasize learning over grades, will increase intrinsic motivation and the use of self-regulated learning strategies. Teaching guidelines and pedagogical examples for enhancing intrinsic motivation are provided.
Enhancing Student Team Effectiveness: Application of Myers-Briggs Personality Assessment in Business Courses
Authors: Amato Christie H. , Amato Louis H.
Published: March 2005

This article examines the relationship between student perceptions of team learning experience and communication style. Student group learning perceptions were evaluated and team communication style was measured using dyads derived from Myers-Briggs personality profiles. Groups containing similar personalities were classified as compatible, whereas groups that blend individuals with different talents and preferences were deemed complementary. Our sample included two sections of Marketing Strategy and one Principles of Macroeconomics section. Macroeconomics students reported greater satisfaction when the group is compatible, whereas Marketing Strategy students preferred complementary groups. The number of group learning experiences and differences in group homogeneity provides likely explanations. Principles of Macroeconomics students, with little or no prior group experience, preferred the comfort of a compatible group. Alternatively, drawing on their considerable group experience, Marketing Strategy students preferred to blend the diverse talents available in a complementary group.
Patenting Marketing Methods: A Missing Topic in the Classroom
Authors: Christ Paul
Published: March 2005

Since the 1998 landmark case that validated the patenting of business methods as intellectual property, a plethora of patents has emerged. Patents for marketing-related processes have been particularly abundant, especially when new marketing processes are tied to a technology component. A review of the marketing literature and textbooks shows an instructor lag in teaching this area. Methods of teaching the area are explored, and suggested materials are noted.
Forensic Marketing: The Use of the Historical Method in a Capstone Marketing Course
Authors: Bussière Dave
Published: March 2005

Case studies have long been used in marketing programs as a way to provide real-world context to business issues and to structure analysis and decision making. In a similar fashion, advocates of the teaching of business/marketing history believe that it provides a contextual background for the marketing student. This article first demonstrates that the historical method is similar to and compatible with the case method. It is then shown that the historical method strengthens analytical skills not used in the case method. Finally, this article further describes an innovative student assignment/project that requires the use of the historical research method.
My Instructor Made Me Do It: Task Characteristics of Procrastination
Authors: Ackerman David S. , Gross Barbara L.
Published: March 2005

Procrastination can have a negative effect on learning. Many previous studies have examined personality factors that contribute to procrastination. This study examines selected assignment characteristics controllable by the instructor that might influence student procrastination. Results found less procrastination on assignments that were perceived as interesting, that required students to use a variety of skills, for which students perceived social norms and rewards for starting promptly, and for which the instructor provided clear instructions. Procrastination was not affected by fear, deadline pressure from other assignments, or the degree to which the task was perceived as difficult or time consuming.
Irregular Marriages: Unorthodox Working-Class Domestic Life in Liverpool, Birmingham, and Manchester, 1900-1939
Authors: Klein Joanne
Published: March 2005

English police personnel records provide a perspective on working-class marriages not available in standard sources. Police officers were respectable workingclass men expected to uphold the reputation of the force both on and off the job, and yet 119 cases of irregular marriages appear in police records in Liverpool, Birmingham, and Manchester. Their affairs, separations, cohabitation, and other unorthodox marital arrangements clearly demonstrate that attempts to impose a strict legal model of marriage on police officers could not displace the more flexible ideas of matrimony still common to respectable working-class culture in the first half of the twentieth century.
Women, Credit, and Family Relationships in England, 1300-1620
Authors: McIntosh Marjorie K.
Published: March 2005

This article examines how women became involved in credit dealings and often in legal action as the result of family relationships between 1300 and 1620. It argues that women’s own personal standing and that of their family underlay their ability to function successfully in a world of financial credit that was based upon delayed obligations. It shows also that women displayed considerable knowledge of economic and legal systems as they attempted to pursue their rights or those of a relative. These observations force one to question any assumption that women’s position within the family was uniformly characterized by deference or reliance upon others.
The Measure of Success: Evidence for Immigrant Networks in the Southern Low Countries, Saint-Omer 1413-1455
Authors: Sortor Marci
Published: March 2005

The importance of immigrants to the demographic and economic survival ofmedieval cities calls for an exploration of how they established themselves in their adopted homes. This study uses network analysis to investigate the social and economic relationships of immigrants who joined the citizenry of Saint-Omer between 1413/14 and 1455/56. Reconstructing the social and economic networks of these new citizens, this study demonstrates that many immigrants did not venture to their new home alone. They either brought with them, or found already established, networks of family and acquaintances that enhanced the likelihood of a successful relocation. These networks could be complex. Immigrants who succeeded in joining Saint-Omer’s bourgeoisie tended to be better supplied with resources, social and economic, than other “outsiders” joining the citizenry. Networks of women immigrants differed somewhat from those of their male counterparts, but social networks were part of women’s successful establishment in their adopted city also.
EFL Professional Development in Cross-Cultural Relief: Examining Effects of U.S. Frameworks on Teacher Practice in México
Authors: Jiang Binbin , DeVillar Robert A.
Published: March 2005

First-year research conducted by the authors of a 3-year, cross-cultural, professional development project involving English-as-a-Foreign Language (EFL) teachers at a private pre-K-12 school in central México. Findings indicate that key elements of the integrative-systematic approach—developed to respond to the combined need for equity and quality schooling within the highly diversified and differentially performing student populations in U.S. public schools—had positive effects on EFL teachers and students within the school.
The Agony and the Ecstasy: Current Status of Hispanic Individuals’ Achievement in Higher Education and Earnings—With a Glimpse to the Future
Authors: Santos Gilberto de los , Asgary Nader , Nazemzadeh Asghar , DeShields Oscar W.
Published: March 2005

Some projections about Hispanic individuals point to a rosy picture regarding gains in higher educational enrollment. Other studies lament that these gains are, at best, minimal. Although the so-called higher education pie is undoubtedly expanding, this article concludes that Hispanic adults are losing, rather than gaining, educational attainment when compared to Black and White adults.
Presence of factors that activate platelet aggregation in mitral stenotic patients' plasma
Authors: Tengiz Istemihan , Ercan Ertugrul , Sahin Fahri , Alioglu Emin , Duman Can , Saydam Guray , Buyukkececi Filiz
Published: March 2005

Background

Although the association between mitral stenosis (MS) and increased coagulation activity is well recognized, it is unclear whether enhanced coagulation remains localized in the left atrium or whether this represents a systemic problem. To assess systemic coagulation parameters and changes in platelet aggregation, we measured fibrinogen levels and performed in vitro platelet function tests in plasma obtained from mitral stenotic patients' and from healthy control subjects' peripheral venous blood.

Methods

Sixteen newly diagnosed patients with rheumatic MS (Group P) and 16 healthy subjects (Group N) were enrolled in the study. Platelet-equalized plasma samples were evaluated to determine in vitro platelet function, using adenosine diphosphate (ADP), collagen and epinephrine in an automated aggregometer. In vitro platelet function tests in group N were performed twice, with and without plasma obtained from group P.

Results

There were no significant differences between the groups with respect to demographic variables. Peripheral venous fibrinogen levels in Group P were not significantly different from those in Group N. Adenosine diphosphate, epinephrine and collagen-induced platelet aggregation ratios were significantly higher in Group P than in Group N. When plasma obtained from Group P was added to Group N subjects' platelets, ADP and collagen-induced, but not epinephrine-induced, aggregation ratios were significantly increased compared to baseline levels in Group N.

Conclusion

Platelet aggregation is increased in patients with MS, while fibrinogen levels remain similar to controls. We conclude that mitral stenotic patients exhibit increased systemic coagulation activity and that plasma extracted from these patients may contain some transferable factors that activate platelet aggregation.
GC-compositional strand bias around transcription start sites in plants and fungi
Authors: Fujimori Shigeo , Washio Takanori , Tomita Masaru
Published: March 2005

Background

A GC-compositional strand bias or GC-skew (=(C-G)/(C+G)), where C and G denote the numbers of cytosine and guanine residues, was recently reported near the transcription start sites (TSS) of Arabidopsis genes. However, it is unclear whether other eukaryotic species have equally prominent GC-skews, and the biological meaning of this trait remains unknown.

Results

Our study confirmed a significant GC-skew (C > G) in the TSS of Oryza sativa (rice) genes. The full-length cDNAs and genomic sequences from Arabidopsis and rice were compared using statistical analyses. Despite marked differences in the G+C content around the TSS in the two plants, the degrees of bias were almost identical. Although slight GC-skew peaks, including opposite skews (C < G), were detected around the TSS of genes in human and Drosophila, they were qualitatively and quantitatively different from those identified in plants. However, plant-like GC-skew in regions upstream of the translation initiation sites (TIS) in some fungi was identified following analyses of the expressed sequence tags and/or genomic sequences from other species. On the basis of our dataset, we estimated that >70 and 68% of Arabidopsis and rice genes, respectively, had a strong GC-skew (>0.33) in a 100-bp window (that is, the number of C residues was more than double the number of G residues in a +/-100-bp window around the TSS). The mean GC-skew value in the TSS of highly-expressed genes in Arabidopsis was significantly greater than that of genes with low expression levels. Many of the GC-skew peaks were preferentially located near the TSS, so we examined the potential value of GC-skew as an index for TSS identification. Our results confirm that the GC-skew can be used to assist the TSS prediction in plant genomes.

Conclusion

The GC-skew (C > G) around the TSS is strictly conserved between monocot and eudicot plants (ie. angiosperms in general), and a similar skew has been observed in some fungi. Highly-expressed Arabidopsis genes had overall a more marked GC-skew in the TSS compared to genes with low expression levels. We therefore propose that the GC-skew around the TSS in some plants and fungi is related to transcription. It might be caused by mutations during transcription initiation or the frequent use of transcription factor-biding sites having a strand preference. In addition, GC-skew is a good candidate index for TSS prediction in plant genomes, where there is a lack of correlation among CpG islands and genes.
Determinants of health insurance ownership among South African women
Authors: Kirigia Joses M , Sambo Luis G , Nganda Benjamin , Mwabu Germano M , Chatora Rufaro , Mwase Takondwa
Published: March 2005

Background

Studies conducted in developed countries using economic models show that individual- and household- level variables are important determinants of health insurance ownership. There is however a dearth of such studies in sub-Saharan Africa. The objective of this study was to examine the relationship between health insurance ownership and the demographic, economic and educational characteristics of South African women.

Methods

The analysis was based on data from a cross-sectional national household sample derived from the South African Health Inequalities Survey (SANHIS). The study subjects consisted of 3,489 women, aged between 16 and 64 years. It was a non-interventional, qualitative response econometric study. The outcome measure was the probability of a respondent's ownership of a health insurance policy.

Results

The χ2 test for goodness of fit indicated satisfactory prediction of the estimated logit model. The coefficients of the covariates for area of residence, income, education, environment rating, age, smoking and marital status were positive, and all statistically significant at p ≤ 0.05. Women who had standard 10 education and above (secondary), high incomes and lived in affluent provinces and permanent accommodations, had a higher likelihood of being insured.

Conclusion

Poverty reduction programmes aimed at increasing women's incomes in poor provinces; improving living environment (e.g. potable water supplies, sanitation, electricity and housing) for women in urban informal settlements; enhancing women's access to education; reducing unemployment among women; and increasing effective coverage of family planning services, will empower South African women to reach a higher standard of living and in doing so increase their economic access to health insurance policies and the associated health services.
Food selection associated with sense of coherence in adults
Authors: Lindmark Ulrika , Stegmayr Birgitta , Nilsson Berit , Lindahl Bernt , Johansson Ingegerd
Published: March 2005

Background

Favorable dietary habits promote health, whereas unfavorable habits link to various chronic diseases. An individual's "sense of coherence" (SOC) is reported to correlate with prevalence of some diseases to which dietary habits are linked. However, understanding what determines an individual's dietary preferences and how to change his/her behavior remains limited. The aim of the present study was to evaluate associations between dietary intake and SOC in adults.

Methods

Diet intake was recorded by an 84-item semi-quantitative food frequency questionnaire and SOC was measured by the 13-item Antonovsky questionnaire in 2,446 men and 2,545 women (25–74 years old) from the population based northern Sweden MONICA screening in 1999.

Results

Intakes of energy, total and saturated fat, ascorbic acid, sucrose, and servings of fruits, vegetables, cereals, and sweets correlated with SOC among women, whereas intakes of total and saturated fat, ascorbic acid, fiber, and alcohol, and servings of fruits, vegetables, bread, bread and cereals, fish, and potatoes correlated with SOC among men. With a few exceptions, intakes of these nutrients/foods were significantly explained by SOC quartile scores in linear GLM models. Both women and men classified into the highest SOC quartile had significantly higher age-BMI-education standardized mean intakes of vegetables than those in the lowest quartiles. Women in the highest SOC quartile also had higher intake of fruits but lower intakes of energy, total and saturated fat, sucrose, and sweets.

Projection to latent structures (PLS) multivariate modeling of intakes of the 84 food items and food aggregates simultaneously on SOC scores supported low SOC to coincide with a presumably less health promoting dietary preference, e.g. intake of pizza, soft drinks, candies, sausages for main course, hamburgers, mashed potato, chips and other snacks, potato salad, French fries, whereas men and women with high SOC scores were characterized by e.g. high intake of rye crisp whole meal bread, boiled potato, vegetables, berries, and fruits.

Conclusion

Both men and women in the highest, as compared with the lowest, SOC score quartile reported more "healthy" food choices. Dietary habits for individuals in the lowest SOC quartile therefore may render a higher risk for various endemic diseases.
L-histidine inhibits production of lysophosphatidic acid by the tumor-associated cytokine, autotaxin
Authors: Clair Timothy , Koh Eunjin , Ptaszynska Malgorzata , Bandle Russell W , Liotta Lance A , Schiffmann Elliott , Stracke Mary L
Published: March 2005

Background

Autotaxin (ATX, NPP-2), originally purified as a potent tumor cell motility factor, is now known to be the long-sought plasma lysophospholipase D (LPLD). The integrity of the enzymatic active site, including three crucial histidine moieties, is required for motility stimulation, as well as LPLD and 5'nucleotide phosphodiesterase (PDE) activities. Except for relatively non-specific chelation agents, there are no known inhibitors of the ATX LPLD activity.

Results

We show that millimolar concentrations of L-histidine inhibit ATX-stimulated but not LPA-stimulated motility in two tumor cell lines, as well as inhibiting enzymatic activities. Inhibition is reversed by 20-fold lower concentrations of zinc salt. L-histidine has no significant effect on the Km of LPLD, but reduces the Vmax by greater than 50%, acting as a non-competitive inhibitor. Several histidine analogs also inhibit the LPLD activity of ATX; however, none has greater potency than L-histidine and all decrease cell viability or adhesion.

Conclusion

L-histidine inhibition of LPLD is not a simple stoichiometric chelation of metal ions but is more likely a complex interaction with a variety of moieties, including the metal cation, at or near the active site. The inhibitory effect of L-histidine requires all three major functional groups of histidine: the alpha amino group, the alpha carboxyl group, and the metal-binding imidazole side chain. Because of LPA's involvement in pathological processes, regulation of its formation by ATX may give insight into possible novel therapeutic approaches.
Preventing complicated transseptal puncture with intracardiac echocardiography: case report
Authors: Shalganov Tchavdar Nikolov , Paprika Dora , Borbás Sarolta , Temesvári András , Szili-Török Tamás
Published: March 2005

Background

Recently, intracardiac echocardiography emerged as a useful tool in the electrophysiology laboratories for guiding transseptal left heart catheterizations, for avoiding thromboembolic and mechanical complications and assessing the ablation lesions characteristics. Although the value of ICE is well known, it is not a universal tool for achieving uncomplicated access to the left atrium. We present a case in which ICE led to interruption of a transseptal procedure because several risk factors for mechanical complications were revealed.

Case presentation

A case of a patient with paroxysmal atrial fibrillation and atrial flutter, and distorted intracardiac anatomy is presented. Intracardiac echocardiography showed a small oval fossa abouting to an enlarged aorta anteriorly. A very small distance from the interatrial septum to the left atrial free wall was seen. The latter two conditions were predisposing to a complicated transseptal puncture. According to fluoroscopy the transseptal needle had a correct position, but the intracardiac echo image showed that it was actually pointing towards the aortic root and most importantly, that it was virtually impossible to stabilize it in the fossa itself. Based on intracardiac echo findings a decision was made to limit the procedure only to ablation of the cavotricuspid isthmus and not to proceed further so as to avoid complications.

Conclusion

This case report illustrates the usefulness of the intracardiac echocardiography in preventing serious or even fatal complications in transseptal procedures when the cardiac anatomy is unusual or distorted. It also helps to understand the possible mechanisms of mechanical complications in cases where fluoroscopic images are apparently normal.
Volume-based non-continuum modeling of bone functional adaptation
Authors: Wang Zhengyuan , Mondry Adrian
Published: March 2005

Background

Bone adapts to mechanical strain by rearranging the trabecular geometry and bone density. The common finite element methods used to simulate this adaptation have inconsistencies regarding material properties at each node and are computationally demanding. Here, a volume-based, non-continuum formulation is proposed as an alternative. Adaptive processes corresponding to various external mechanical loading conditions are simulated for the femur.

Results

Bone adaptations were modeled for one-legged stance, abduction and adduction. One-legged stance generally results in higher bone densities than the other two loading cases. The femoral head and neck are the regions where densities change most drastically under different loading conditions while the distal area always contains the lowest densities regardless of the loading conditions. In the proposed formulation, the inconsistency of material densities or strain energy densities, which is a common problem to finite element based approaches, is eliminated. The computational task is alleviated through introduction of the quasi-binary connectivity matrix and linearization operations in the Jacobian matrix and is therefore computationally less demanding.

Conclusion

The results demonstrated the viability of the proposed formulation to study bone functional adaptation under mechanical loading.
Aggressive HIV-1?
Authors: Berkhout Ben , de Ronde Anthony , van der Hoek Lia
Published: March 2005

New York City health officials announced on February 11, 2005 that a patient rapidly developed full-blown AIDS shortly after being diagnosed with a rare, drug-resistant strain of HIV-1. The New York City Department of Health issued an alert to all hospitals and doctors and a press conference was held to announce the emergence of an aggressive HIV-1 strain that may be difficult to treat and that appears to trigger rapid progression to AIDS. Is the panic justified?
Phosphorylation of HIV Tat by PKR increases interaction with TAR RNA and enhances transcription
Authors: Endo-Munoz Liliana , Warby Tammra , Harrich David , McMillan Nigel A J
Published: March 2005

Background

The interferon (IFN)-induced, dsRNA-dependent serine/threonine protein kinase, PKR, plays a key regulatory role in the IFN-mediated anti-viral response by blocking translation in the infected cell by phosphorylating the alpha subunit of elongation factor 2 (eIF2). The human immunodeficiency virus type 1 (HIV-1) evades the anti-viral IFN response through the binding of one of its major transcriptional regulatory proteins, Tat, to PKR. HIV-1 Tat acts as a substrate homologue for the enzyme, competing with eIF2α, and inhibiting the translational block. It has been shown that during the interaction with PKR, Tat becomes phosphorylated at three residues: serine 62, threonine 64 and serine 68. We have investigated the effect of this phosphorylation on the function of Tat in viral transcription. HIV-1 Tat activates transcription elongation by first binding to TAR RNA, a stem-loop structure found at the 5' end of all viral transcripts. Our results showed faster, greater and stronger binding of Tat to TAR RNA after phosphorylation by PKR.

Results

We have investigated the effect of phosphorylation on Tat-mediated transactivation. Our results showed faster, greater and stronger binding of Tat to TAR RNA after phosphorylation by PKR. In vitro phosphorylation experiments with a series of bacterial expression constructs carrying the wild-type tat gene or mutants of the gene with alanine substitutions at one, two, or all three of the serine/threonine PKR phosphorylation sites, showed that these were subject to different levels of phosphorylation by PKR and displayed distinct kinetic behaviour. These results also suggested a cooperative role for the phosphorylation of S68 in conjunction with S62 and T64. We examined the effect of phosphorylation on Tat-mediated transactivation of the HIV-1 LTR in vivo with a series of analogous mammalian expression constructs. Co-transfection experiments showed a gradual reduction in transactivation as the number of mutated phosphorylation sites increased, and a 4-fold decrease in LTR transactivation with the Tat triple mutant that could not be phosphorylated by PKR. Furthermore, the transfection data also suggested that the presence of S68 is necessary for optimal Tat-mediated transactivation.

Conclusion

These results support the hypothesis that phosphorylation of Tat may be important for its function in HIV-1 LTR transactivation.
In at the deep end: making the transition from SpR to consultant
Authors: Wilkie Grant , Raffaelli Derek
Published: March 2005

The first few years of a consultant's professional life can be difficult for all concerned. In this article we argue that the tasks facing the newly appointed consultant are in part instrumental, but that there are also important emotional and development challenges to be faced before the individual psychiatrist can truly fill out the consultant role. For the past 2 years we have been running a group, which we have called the transition group, for specialist registrars and newly appointed consultants working in the West of Scotland. The aim of this group is to provide peer support during this transitional process. Here we draw on our experience in this group to explore the development of professional identity, the exercise of personal authority and how this process can be supported by a group such as ours.
Re-evaluating confidentiality: using patient information in teaching and publications
Authors: Draper Heather , Rogers Wendy
Published: March 2005

Case studies used in psychiatry for teaching and learning can include large amounts of personal information, and most medical journals refuse to publish case material without patient consent. However, not all patients are capable of consenting and maintaining anonymity is not always successful. Disclosure of personal information without consent can cause a sense of violation, but a principle of non-violation rigorously applied would prohibit the use of such material without consent, even if the patient is completely unidentifiable. A public interest argument can be made for using patient information for teaching purposes. Furthermore, a limited case can be made for using, and even publishing, some cases without consent, e.g. those requiring urgent public discussion or concerning people who are incapable of giving consent. Thus, an argument can be put forward for relaxing to this limited degree the demand for patient consent to publish case material.
Lifestyle and physical health in schizophrenia
Authors: Connolly Moira , Kelly Ciara
Published: March 2005

People with schizophrenia die prematurely. Their illness, its treatment and their lifestyle all contribute to the excess morbidity and mortality. Lifestyle ‘choices' (e.g. poor diet, low rates of physical activity and increased likelihood to smoke cigarettes) predispose them to poor physical health and comorbid medical diseases. In addition, weight gain and obesity are a consequence of most antipsychotics, particularly the atypicals. Excessive body weight increases the risk of morbidity and mortality, and is the biggest risk factor for type II diabetes in schizophrenia. Much of the excess mortality of schizophrenia is preventable through lifestyle and risk factor modification and the treatment of common diseases, but the potential for improving outcomes in this area is only starting to be addressed.
Shared care, individual expertise: Invited commentary on… Shared care for people with mental illness
Authors: Burns Tom
Published: March 2005

‘Shared care in mental health is now a policy imperative in England and Wales, yet its meaning … [is] … still open to debate’. Dr Lester's opening line says it all (Lester, 2005, this issue). We're all in favour of it, but none of us is exactly sure what it is. Ambiguity is considered a failing in academic publications and, under pressure from editors, we usually excise the unnecessary and unclear. Politicians, however, often deliberately insert ambiguity, as it allows scope for interpretation. By a simple shift in emphasis, either shared care's achievement can be claimed as a manifesto success or slow progress towards it can be cited to justify further targets.
Physical examination in psychiatric practice
Authors: Garden Gill
Published: March 2005

Physical disease is more prevalent in people with mental disorder than in the general population. It is important for psychiatrists to maintain skills in physical examination to ensure that physical illness is diagnosed and treated appropriately. A sound knowledge of medical illness ensures that examination is targeted at the relevant diseases. Mental health units should provide adequate facilities and equipment. All detailed examinations should be undertaken in the presence of a chaperone. Opportunities for psychiatrists to refresh their knowledge and skill are suggested.
Suicide risk: structured professional judgement
Authors: Bouch Joe , Marshall John James
Published: March 2005

Patient risk factors for suicide are well known to psychiatrists, yet the availability of clinically useful, routine and systematic methods for risk recognition are limited. This article outlines the structured professional judgement approach to suicide risk assessment and management. This method combines psychiatric assessment and formulation with the evidence base for suicide risk factors. Structured professional judgement is contrasted with actuarial and clinical judgement approaches. A categorisation of risk factors is presented, with four groups described – static, stable, dynamic and future. Case histories illustrate long-term high risk contrasted with sudden and unpredictable onset of suicidality.
Understanding and treating depersonalisation disorder
Authors: Medford Nick , Sierra Mauricio , Baker Dawn , David Anthony S.
Published: March 2005

Depersonalisation disorder involves an unpleasant, chronic and disabling alteration in the experience of self and environment. In addition to these classic features of depersonalisation and derealisation, symptoms may also encompass alterations in bodily sensation and a loss of emotional reactivity. Primary depersonalisation disorder is probably more common than previously thought, and here we discuss the diagnosis, assessment and treatment of the condition, with particular reference to our experiences in a specialist depersonalisation clinic. We also consider psychological and biological aspects of the condition. Although there is as yet no recognised treatment for this disorder, various pharmacological interventions, particularly a combination of lamotrigine and a selective serotonin reuptake inhibitor, have shown promise. We discuss these drug treatments, together with psychological approaches, in particular a recent cognitive–behavioural conceptualisation and treatment approach.