bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Shear Tabs and High-Strength Bolts in Seismic Performance of Steel Moment Connections
Authors: Lai Chi-Ming , Yeh Ching-Yu , Kang Sin-Yu , Chang Heui-Yung
Published: September 2021

A shear tab and high-strength bolts are often used to connect a steel H-beam to a column. The design demand and capacity of these elements vary from one standard to the other. To investigate the effect, this study applied a finite element method (FEM) to develop models for two steel moment connections and validated the effectiveness by test data. The connections were characteristic of bolted-web-and-welded-flange details. The FEM models were then used to study the design of shear tabs and high-strength bolts in accordance with the U.S. and Japan standards and compared to the Taiwan practice. The result showed a small difference in the peak loads of the connections. However, the U.S. direct welded flange connection had flange buckling and strength degradation at a relatively smaller drift. The connection had a thinner shear tab and fewer high-strength bolts. The other two connections had very similar design results and loading responses. The increase in shear-tab thickness reduced the stress concentration and fracture potential of the connections. It is, therefore, recommended to design a shear tab with moment capacity greater than the beam web. This will reduce the stress concentration of the base metal surrounding the beam-flange groove welds, increasing the connection ductility.
Graphene-Enhanced Battery Components in Rechargeable Lithium-Ion and Lithium Metal Batteries
Authors: Chang Hao-Hsun , Ho Tseng-Hsiang , Su Yu-Sheng
Published: September 2021

Stepping into the 21st century, “graphene fever” swept the world due to the discovery of graphene, made of single-layer carbon atoms with a hexagonal lattice. This wonder material displays impressive material properties, such as its electrical conductivity, thermal conductivity, and mechanical strength, and it also possesses unique optical and magnetic properties. Many researchers see graphene as a game changer for boosting the performance of various applications. Emerging consumer electronics and electric vehicle technologies require advanced battery systems to enhance their portability and driving range, respectively. Therefore, graphene seems to be a great candidate material for application in high-energy-density/high-power-density batteries. The “graphene battery”, combining two Nobel Prize-winning concepts, is also frequently mentioned in the news and articles all over the world. This review paper introduces how graphene can be adopted in Li-ion/Li metal battery components, the designs of graphene-enhanced battery materials, and the role of graphene in different battery applications.
Metabolic Hormones Modulate Macrophage Inflammatory Responses
Authors: Batty Matthew J. , Chabrier Gwladys , Sheridan Alanah , Gage Matthew C.
Published: September 2021

Macrophages are phagocytotic leukocytes that play an important role in the innate immune response and have established roles in metabolic diseases and cancer progression. Increased adiposity in obese individuals leads to dysregulation of many hormones including those whose functions are to coordinate metabolism. Recent evidence suggests additional roles of these metabolic hormones in modulating macrophage inflammatory responses. In this review, we highlight key metabolic hormones and summarise their influence on the inflammatory response of macrophages and consider how, in turn, these hormones may influence the development of different cancer types through the modulation of macrophage functions.
CAR-T after Stem Cell Transplantation in B-Cell Lymphoproliferative Disorders: Are They Really Autologous or Allogenic Cell Therapies?
Authors: Bartoló-Ibars Ariadna , Uribe-Herranz Mireia , Muñoz-Sánchez Guillermo , Arnaldos-Pérez Cristina , Ortiz-Maldonado Valentín , Urbano-Ispizua Álvaro , Pascal Mariona , Juan Manel
Published: September 2021

Allogenic hematopoietic stem cell transplantation (allo-HSCT) is one of the standard treatments for B-cell lymphoproliferative disorders; however, deep relapses are common after an allo-HSCT, and it is associated with poor prognosis. A successful approach to overcome these relapses is to exploit the body’s own immune system with chimeric antigen receptor (CAR) T-cells. These two approaches are potentially combinatorial for treating R/R B-cell lymphoproliferative disorders. Several clinical trials have described different scenarios in which allo-HSCT and CAR-T are successively combined. Further, for all transplanted patients, assessment of chimerism is important to evaluate the engraftment success. Nonetheless, for those patients who previously received an allo-HSCT there is no monitorization of chimerism before manufacturing CAR T-cells. In this review, we focus on allo-HSCT and CAR-T treatments and the different sources of T-cells for manufacturing CAR T-cells.
Gut Microbiome Modulation and Faecal Microbiota Transplantation Following Allogenic Hematopoietic Stem Cell Transplantation
Authors: Kaźmierczak-Siedlecka Karolina , Skonieczna-Żydecka Karolina , Biliński Jarosław , Roviello Giandomenico , Iannone Luigi Francesco , Atzeni Alessandro , Sobocki Bartosz Kamil , Połom Karol
Published: September 2021

Nowadays, allogenic hematopoietic stem cell transplantation (allo-HSCT) is a curative therapy that is mainly recommended for hematologic malignancies. However, complications (such as graft-versus-host disease, mucositis, disease relapse, and infections) associated with the HSCT procedure contribute to the development of gut microbiota imbalance, gut-barrier disruption, and increased intestinal permeability. In the present narrative review, the crosstalk between gut microbiota products and intestinal homeostasis is discussed. Notably, gut-microbiota-related aspects have an impact on patients’ clinical outcomes and overall survival. In accordance with the most recent published data, gut microbiota is crucial for the treatment effectiveness of many diseases, not only gastrointestinal cancers but also hematologic malignancies. Therefore, it is necessary to indicate a therapeutic method allowing to modulate gut microbiota in HSCT recipients. Currently, fecal microbiota transplantation (FMT) is the most innovative method used to alter/restore gut microbiota composition, as well as modulate its activity. Despite the fact that some previous data have shown promising results, the knowledge regarding FMT in HSCT is still strongly limited, except for the treatment of Clostridium difficile infection. Additionally, administration of prebiotics, probiotics, synbiotics, and postbiotics can also modify gut microbiota; however, this strategy should be considered carefully due to the high risk of fungemia/septicemia (especially in case of fungal probiotics).
Rapid Progress in the Use of Immunomodulatory Drugs and Cereblon E3 Ligase Modulators in the Treatment of Multiple Myeloma
Authors: Charliński Grzegorz , Vesole David H. , Jurczyszyn Artur
Published: September 2021

Over the past two decades, the improvement in our understanding of the biology of MM and the introduction of new drug classes, including immunomodulatory drugs (IMiDs), proteasome inhibitors (PI), and monoclonal antibodies (MoAb), have significantly improved outcomes. The first IMiD introduced to treat MM was thalidomide. The side effects observed during treatment with thalidomide initiated work on the synthesis of IMiD analogs. Subsequently, lenalidomide and pomalidomide were developed, both with different safety profiles, and they have better tolerability than thalidomide. In 2010, the cereblon (CRBN) protein was discovered as a direct target of IMiDs. By binding to CRBN, IMiDs change the substrate specificity of the CRBN E3 ubiquitin ligase complex, which results in the breakdown of internal Ikaros and Aiolos proteins. Most clinical trials conducted, both in newly diagnosed, post-transplant maintenance and relapsed/refractory MM, report a beneficial effect of IMiDs on the extension of progression-free survival and overall survival in patients with MM. Due to side effects, thalidomide is used less frequently. Currently, lenalidomide is used at every phase of MM treatment. Lenalidomide is used in conjunction with other agents such as PIs and MoAb as induction and relapsed therapy. Pomalidomide is currently used to treat relapsed/refractory MM, also with PIs and monoclonal antibodies. Current clinical trials are evaluating the efficacy of IMiD derivatives, the CRBN E3 ligase modulators (CELMoDs). This review focuses on the impact of IMiDs for the treatment of MM.
Multiple Myeloma Relapse Is Associated with Increased NFκB Pathway Activity and Upregulation of the Pro-Survival BCL-2 Protein BFL-1
Authors: Spaan Ingrid , van de Stolpe Anja , Raymakers Reinier A. , Peperzak Victor
Published: September 2021

Multiple myeloma (MM) is a hematological malignancy that is still considered incurable due to the development of therapy resistance and subsequent relapse of disease. MM plasma cells (PC) use NFκB signaling to stimulate cell growth and disease progression, and for protection against therapy-induced apoptosis. Amongst its diverse array of target genes, NFκB regulates the expression of pro-survival BCL-2 proteins BCL-XL, BFL-1, and BCL-2. A possible role for BFL-1 in MM is controversial, since BFL-1, encoded by BCL2A1, is downregulated when mature B cells differentiate into antibody-secreting PC. NFκB signaling can be activated by many factors in the bone marrow microenvironment and/or induced by genetic lesions in MM PC. We used the novel signal transduction pathway activity (STA) computational model to quantify the functional NFκB pathway output in primary MM PC from diverse patient subsets at multiple stages of disease. We found that NFκB pathway activity is not altered during disease development, is irrespective of patient prognosis, and does not predict therapy outcome. However, disease relapse after treatment resulted in increased NFκB pathway activity in surviving MM PC, which correlated with increased BCL2A1 expression in a subset of patients. This suggests that BFL-1 upregulation, in addition to BCL-XL and BCL-2, may render MM PC resistant to therapy-induced apoptosis, and that BFL-1 targeting could provide a new approach to reduce therapy resistance in a subset of relapsed/refractory MM patients.
Patterns of Extrapulmonary Metastases in Sarcoma Surveillance
Authors: Hong Zachery , England Patrick , Rhea Lee , Hirbe Angela , McDonald Douglas , Cipriano Cara A.
Published: September 2021

Soft tissue sarcomas (STS) most commonly metastasize to the lungs. Current surveillance guidelines variably recommend abdominal and pelvic imaging, but there is little evidence to support this. We sought to determine the proportion of initial pulmonary versus extrapulmonary metastases, the time to development of each, and factors to identify patients that would benefit from abdominopelvic surveillance. We retrospectively reviewed 382 patients who underwent surgical treatment for STS at a single institution. Of the 33% (126/382) of patients who developed metastases, 72% (90/126) were pulmonary, 22% (28/126) were extrapulmonary, and 6% (8/126) developed both simultaneously. Initial extrapulmonary metastases occurred later (log rank p = 0.049), with median 11 months (IQR, 5 to 19) until pulmonary disease and 22 months (IQR, 6 to 45) until extrapulmonary disease. Pulmonary metastases were more common in patients with high grade tumors (p = 0.0201) and larger tumors (p < 0.0001). Our multivariate analysis did not identify any factors associated with initial extrapulmonary metastases. A substantial minority of initial metastases were extrapulmonary; these occurred later and over a broader time range than initial pulmonary metastases. Moreover, extrapulmonary metastases are more difficult to predict than pulmonary metastases, adding to the challenge of creating targeted surveillance protocols.
Integrative Transcriptome Profiling Reveals SKA3 as a Novel Prognostic Marker in Non-Muscle Invasive Bladder Cancer
Authors: You Chaelin , Piao Xuan-Mei , Kang Keunsoo , Kim Yong-June , Kang Kyuho
Published: September 2021

Approximately 80% of all new bladder cancer patients are diagnosed with non-muscle invasive bladder cancer (NMIBC). However, approximately 15% of them progress to muscle-invasive bladder cancer (MIBC), for which prognosis is poor. The current study aimed to improve diagnostic accuracy associated with clinical outcomes in NMIBC patients. Nevertheless, it has been challenging to identify molecular biomarkers that accurately predict MIBC progression because this disease is complex and heterogeneous. Through integrative transcriptome profiling, we showed that high SKA3 expression is associated with poor clinical outcomes and MIBC progression. We performed RNA sequencing on human tumor tissues to identify candidate biomarkers in NMIBC. We then selected genes with prognostic significance by analyzing public datasets from multiple cohorts of bladder cancer patients. We found that SKA3 was associated with NMIBC pathophysiology and poor survival. We analyzed public single-cell RNA-sequencing (scRNA-seq) data for bladder cancer to dissect transcriptional tumor heterogeneity. SKA3 was expressed in an epithelial cell subpopulation expressing genes regulating the cell cycle. Knockdown experiments confirmed that SKA3 promotes bladder cancer cell proliferation by accelerating G2/M transition. Hence, SKA3 is a new prognostic marker for predicting NMIBC progression. Its inhibition could form part of a novel treatment lowering the probability of bladder cancer progression.
VKNG-1 Antagonizes ABCG2-Mediated Multidrug Resistance via p-AKT and Bcl-2 Pathway in Colon Cancer: In Vitro and In Vivo Study
Authors: Narayanan Silpa , Fan Ying-Fang , Gujarati Nehaben A. , Teng Qiu-Xu , Wang Jing-Quan , Cai Chao-Yun , Yang Yuqi , Chintalapati Anirudh J. , Lei Yixiong , Korlipara Vijaya L. , Chen Zhe-Sheng
Published: September 2021

The emergence of multidrug resistance (MDR) to chemotherapeutic drugs is a major problem in the therapy of cancer. Knowledge of the mechanisms of drug resistance in cancer is necessary for developing efficacious therapies. ATP-binding cassette (ABC) transporters are transmembrane proteins that efflux chemotherapeutic drugs from cancer cells, thereby producing MDR. Our research efforts have led to the discovery of VKNG-1, a compound that selectively inhibits the ABCG2 transporter and reverses resistanctabe to standard anticancer drugs both in vitro and in vivo. VKNG-1, at 6 µM, selectively inhibited ABCG2 transporter and sensitized ABCG2-overexpressing drug-resistant cancer cells to the ABCG2 substrate anticancer drugs mitoxantrone, SN-38, and doxorubicin in ABCG2-overexpressing colon cancers. VKNG- 1 reverses ABCG2-mediated MDR by blocking ABCG2 efflux activity and downregulating ABCG2 expression at the mRNA and protein levels. Moreover, VKNG-1 inhibits the level of phosphorylated protein kinase B (PKB/p-AKT), and B-cell lymphoma-2 (Bcl-2) protein which may overcome resistance to anticancer drugs. However, the in vitro translocation of ABCG2 protein did not occur in the presence of 6 µM of VKNG-1. In addition, VKNG-1 enhanced the anticancer efficacy of irinotecan in ABCG2- overexpressing mouse tumor xenografts. Overall, our results suggest that VKNG-1 may, in combination with certain anticancer drugs, represent a treatment to overcome ABCG2-mediated MDR colon cancers.
Dynamic Expression of Membrane Type 1-Matrix Metalloproteinase (Mt1-mmp/Mmp14) in the Mouse Embryo
Authors: Muñoz-Sáez Emma , Moracho Natalia , Learte Ana I. R. , Arroyo Alicia G. , Sánchez-Camacho Cristina
Published: September 2021

MT1-MMP/MMP14 belongs to a subgroup of the matrix metalloproteinases family that presents a transmembrane domain, with a cytosolic tail and the catalytic site exposed to the extracellular space. Deficient mice for this enzyme result in early postnatal death and display severe defects in skeletal, muscle and lung development. By using a transgenic line expressing the LacZ reporter under the control of the endogenous Mt1-mmp promoter, we reported a dynamic spatiotemporal expression pattern for Mt1-mmp from early embryonic to perinatal stages during cardiovascular development and brain formation. Thus, Mt1-mmp shows expression in the endocardium of the heart and the truncus arteriosus by E8.5, and is also strongly detected during vascular system development as well as in endothelial cells. In the brain, LacZ reporter expression was detected in the olfactory bulb, the rostral cerebral cortex and the caudal mesencephalic tectum. LacZ-positive cells were observed in neural progenitors of the spinal cord, neural crest cells and the intersomitic region. In the limb, Mt1-mmp expression was restricted to blood vessels, cartilage primordium and muscles. Detection of the enzyme was confirmed by Western blot and immunohistochemical analysis. We suggest novel functions for this metalloproteinase in angiogenesis, endocardial formation and vascularization during organogenesis. Moreover, Mt1-mmp expression revealed that the enzyme may contribute to heart, muscle and brain throughout development.
Molecular Interactions Driving Intermediate Filament Assembly
Authors: Vermeire Pieter-Jan , Stalmans Giel , Lilina Anastasia V. , Fiala Jan , Novak Petr , Herrmann Harald , Strelkov Sergei V.
Published: September 2021

Given the role of intermediate filaments (IFs) in normal cell physiology and scores of IF-linked diseases, the importance of understanding their molecular structure is beyond doubt. Research into the IF structure was initiated more than 30 years ago, and some important advances have been made. Using crystallography and other methods, the central coiled-coil domain of the elementary dimer and also the structural basis of the soluble tetramer formation have been studied to atomic precision. However, the molecular interactions driving later stages of the filament assembly are still not fully understood. For cytoplasmic IFs, much of the currently available insight is due to chemical cross-linking experiments that date back to the 1990s. This technique has since been radically improved, and several groups have utilized it recently to obtain data on lamin filament assembly. Here, we will summarize these findings and reflect on the remaining open questions and challenges of IF structure. We argue that, in addition to X-ray crystallography, chemical cross-linking and cryoelectron microscopy are the techniques that should enable major new advances in the field in the near future.
Weight Pulling: A Novel Mouse Model of Human Progressive Resistance Exercise
Authors: Zhu Wenyuan G. , Hibbert Jamie E. , Lin Kuan Hung , Steinert Nathaniel D. , Lemens Jake L. , Jorgenson Kent W. , Newman Sarah M. , Lamming Dudley W. , Hornberger Troy A.
Published: September 2021

This study describes a mouse model of progressive resistance exercise that utilizes a full-body/multi-joint exercise (weight pulling) along with a training protocol that mimics a traditional human paradigm (three training sessions per week, ~8–12 repetitions per set, 2 min of rest between sets, approximately two maximal-intensity sets per session, last set taken to failure, and a progressive increase in loading that is based on the individual’s performance). We demonstrate that weight pulling can induce an increase in the mass of numerous muscles throughout the body. The relative increase in muscle mass is similar to what has been observed in human studies, and is associated with the same type of long-term adaptations that occur in humans (e.g., fiber hypertrophy, myonuclear accretion, and, in some instances, a fast-to-slow transition in Type II fiber composition). Moreover, we demonstrate that weight pulling can induce the same type of acute responses that are thought to drive these long-term adaptations (e.g., the activation of signaling through mTORC1 and the induction of protein synthesis at 1 h post-exercise). Collectively, the results of this study indicate that weight pulling can serve as a highly translatable mouse model of progressive resistance exercise.
The Role of Stem Cells and Their Derived Extracellular Vesicles in Restoring Female and Male Fertility
Authors: Qamar Ahmad Yar , Hussain Tariq , Rafique Muhammad Kamran , Bang Seonggyu , Tanga Bereket Molla , Seong Gyeonghwan , Fang Xun , Saadeldin Islam M. , Cho Jongki
Published: September 2021

Infertility is a globally recognized issue caused by different reproductive disorders. To date, various therapeutic approaches to restore fertility have been attempted including etiology-specific medication, hormonal therapies, surgical excisions, and assisted reproductive technologies. Although these approaches produce results, however, fertility restoration is not achieved in all cases. Advances in using stem cell (SC) therapy hold a great promise for treating infertile patients due to their abilities to self-renew, differentiate, and produce different paracrine factors to regenerate the damaged or injured cells and replenish the affected germ cells. Furthermore, SCs secrete extracellular vesicles (EVs) containing biologically active molecules including nucleic acids, lipids, and proteins. EVs are involved in various physiological and pathological processes and show promising non-cellular therapeutic uses to combat infertility. Several studies have indicated that SCs and/or their derived EVs transplantation plays a crucial role in the regeneration of different segments of the reproductive system, oocyte production, and initiation of sperm production. However, available evidence triggers the need to testify the efficacy of SC transplantation or EVs injection in resolving the infertility issues of the human population. In this review, we highlight the recent literature covering the issues of infertility in females and males, with a special focus on the possible treatments by stem cells or their derived EVs.
Chirality Transfer in a Calixarene-Based Directional Pseudorotaxane Complex
Authors: Concilio Gerardo , Gaeta Carmine , Della Sala Paolo , Iuliano Veronica , Talotta Carmen , Monaco Guglielmo , Superchi Stefano , Belviso Sandra , Neri Placido
Published: September 2021

Hexamethoxycalix[6]arene 3 forms a directional pseudorotaxane complex with the chiral axle (S)-(α-methyl-benzyl)benzylammonium 2+. Between the two (endo-chiral)-2+@3 and (exo-chiral)-2+@3 pseudorotaxane stereoisomers, the former is preferentially formed. This result confirms the validity of the “endo-α-methyl-benzyl rule”, previously reported by us. DFT calculations suggest that C-H … π interactions between the methyl group of 2+ and the calixarene aromatic rings, determine the stereoselectivity of the threading process toward the “endo-α-methyl-benzyl preference”. An amplification of optical rotation is observed upon formation of the pseudorotaxane complex (endo-chiral)-2+@3 with respect to free axle 2+. Thus, the specifical rotation of the 1:1 mixture of chiral 2+·B(ArF)4− salt and achiral 3 was augmented upon formation of the pseudorotaxane and DFT calculations were used to rationalize this result.
Core/Shell Glycine-Polyvinyl Alcohol/Polycaprolactone Nanofibrous Membrane Intended for Guided Bone Regeneration: Development and Characterization
Authors: Alazzawi Marwa , Kadim Abid Alsahib Nabeel , Turkoglu Sasmazel Hilal
Published: September 2021

Glycine (Gly), which is the simplest amino acid, induces the inflammation response and enhances bone mass density, and particularly its β polymorph has superior mechanical and piezoelectric properties. Therefore, electrospinning of Gly with any polymer, including polyvinyl alcohol (PVA), has a great potential in biomedical applications, such as guided bone regeneration (GBR) application. However, their application is limited due to a fast degradation rate and undesirable mechanical and physical properties. Therefore, encapsulation of Gly and PVA fiber within a poly(ε-caprolactone) (PCL) shell provides a slower degradation rate and improves the mechanical, chemical, and physical properties. A membrane intended for GBR application is a barrier membrane used to guide alveolar bone regeneration by preventing fast-proliferating cells from growing into the bone defect site. In the present work, a core/shell nanofibrous membrane, composed of PCL as shell and PVA:Gly as core, was developed utilizing the coaxial electrospinning technique and characterized morphologically, mechanically, physically, chemically, and thermally. Moreover, the characterization results of the core/shell membrane were compared to monolithic electrospun PCL, PVA, and PVA:Gly fibrous membranes. The results showed that the core-shell membrane appears to be a good candidate for GBR application with a nano-scale fiber of 412 ± 82 nm and microscale pore size of 6.803 ± 0.035 μm. Moreover, the wettability of 47.4 ± 2.2° contact angle (C.A) and mechanical properties of 135 ± 3.05 MPa average modulus of elasticity, 4.57 ± 0.04 MPa average ultimate tensile stress (UTS), and 39.43% ± 0.58% average elongation at break are desirable and suitable for GBR application. Furthermore, the X-ray diffraction (XRD) and transmission electron microscopy (TEM) results exhibited the formation of β-Gly.
Mathematical Simulation and Experimental Verification of Carburizing Quenching Process Based on Multi-Field Coupling
Authors: Wang Jiangang , Yang Shuai , Li Jianhui , Ju Dongying , Li Xusheng , He Fangbo , Li Hui , Chen Yong
Published: September 2021

Based on the multi-field coupling effect of temperature, diffusion, and phase change, the finite element model of carburizing and quenching was established. The 20CrMnTiH steel helical gear as the research object, prediction accuracy of carburizing, and quenching model of complex helical gear was studied. The material properties database of experimental steel was established by JMatPro, and the material thermophysical parameters needed in the calculation process were obtained. The carburizing and quenching process of transmission helical gear was numerically simulated by thermodynamic three-dimensional coupling analysis method combined with actual heat treatment process. The microstructure morphology, macro hardness, and deformation were characterized. The experimental results show that the microstructure of the hardened surface layer was acicular martensite and a small amount of residual austenite. The highest hardness appears at the surface layer of 778.8 HV, the effective hardened layer depth was 0.9 mm, and the maximum deformation of the gear was 0.055 mm. By comparing the experimental measurement results with the simulation results, they were in good agreement, which verifies the accuracy of the finite element model. This indicates that the model has good prediction ability in carburizing and quenching process.
Skin Care Formulations and Lipid Carriers as Skin Moisturizing Agents
Authors: Pavlou Panagoula , Siamidi Angeliki , Varvaresou Athanasia , Vlachou Marilena
Published: September 2021

Skin care formulations have evolved as the interaction of health and beauty products for the skin. Their benefits are based on the combination of cosmetic active ingredients and targeted application. Cosmetic actives have been used in novel formulations for decades (sunscreens, anti-aging treatments, etc.), but the problems with their low solubility, low penetration, and physicochemical instability when applied to the skin have yet to be solved. One way to circumvent these shortcomings is to use lipid carriers, which are known to play an important role in the solubility of poorly soluble compounds by facilitating skin permeation and improving stability. This review addresses recent advances in skin care products that use novel nanotechnology-based lipid systems (liposomes, solid lipid nanoparticles, etc.) to deliver moisturizing cosmetic actives and improve product efficacy.
Preparation of Readily-to-Use Stilbenoids Extract from Morus alba Callus Using a Natural Deep Eutectic Solvent
Authors: Komaikul Jukrapun , Mangmool Supachoke , Putalun Waraporn , Kitisripanya Tharita
Published: September 2021

The consumer and cosmetic industries have recently placed a greater emphasis on ecofriendly solvents for botanical extraction, including natural deep eutectic solvents (NADES). In this study, NADES were prepared for Morus alba callus extraction. The efficiency of extraction from the NADES and methanol was investigated by comparison of the stilbenoids yield and anti-melanogenesis activity. Prior to testing the irritability of a suitable NADES on the reconstructed human epidermis (RhE), the effect of the selected NADES on stilbenoids stability was determined. The results showed that the highest yields of stilbenoids were obtained from choline chloride-glycerol mixtures (Ch1G2) and methanol extracts, with no significant difference in yields (5.06 ± 0.05 and 6.32 ± 0.40 mg/g callus dry weight, respectively). The NADES extracts of M. alba callus showed comparable anti-melanogenesis activity compared to methanol. In term of stability, stilbenoids in Ch1G2 remained stable after six months of storage at 4 °C except resveratrol. Furthermore, Ch1G2 had no irritation effect on RhE. Thus, based on the findings of this study, Ch1G2 is an intriguing green solvent alternative for the extraction of M. alba callus and may be advantageous for the preparation of skin-lightening cosmetics.
Infrared Thermography as a Diagnostic Tool for the Assessment of Patients with Symptomatic Peripheral Arterial Disease Undergoing Infrafemoral Endovascular Revascularisations
Authors: Zenunaj Gladiol , Lamberti Nicola , Manfredini Fabio , Traina Luca , Acciarri Pierfilippo , Bisogno Francesca , Scian Sabrina , Serra Raffaele , Abatangelo Giulio , Gasbarro Vincenzo
Published: September 2021

Aim: The aim of this study was to evaluate the utility and reliability of temperature foot changes measured by infrared thermography (IRT) for the evaluation of patients with atherosclerotic peripheral arterial disease (PAD) before and after endovascular revascularisation. Methods: This is an observational prospective study carried out on symptomatic PAD patients. Evaluations consisted of a clinical examination, duplex scan with ankle–brachial index calculation (ABI) and IRT measurements with infrared camera FLIR-ONE connected to a smartphone with android technology. Locations on the foot sampled with IRT were the anterior tibial, pedal, posterior and arcuate arteries. Results obtained with IRT on the symptomatic foot were compared to the contralateral foot and with the ABI values obtained bilaterally before and 24 h after revascularisation. Results: Within one year, 40 patients were enrolled, among whom 87,5% suffered from critical limb ischaemia. In three patients, it was impossible to obtain ABI measurements because of ulcerations on the limb. Skin temperature changes obtained by IRT between the symptomatic limb and the contralateral limb had a mean difference of 1.7 °C (range: 1.1–2.2 °C), p < 0.001. There was a positive correlation between ABI and temperature values of the limb needed for treatment before revascularisation (p = 0.025; r = 0.36) and after revascularisation (p = 0.024, r = 0.31). The technical success rate was 100% in all cases, achieving a significant increase in temperature at all points of the foot analysed, with a median change of 2 °C (p < 0.001). Conclusion: IRT is a safe, reliable and simple application. It could be a valuable tool for the assessment of the clinical presentation and severity of foot blood perfusion in symptomatic PAD patients and the evaluation of the technical success of endovascular revascularisation. IRT might have a role in follow-up of revascularisation procedures.
A Curve Maybe to Narrow: Description of an Anomalous Course of the Right Coronary Artery
Authors: Pastorini Guido , Bertone Elisa , Talenti Alberto , Feola Mauro
Published: September 2021

Congenital coronary artery anomalies are rare but well-described causes of chest pain and, in some cases, link to sudden cardiac death. With the spread of advanced imaging techniques, the number of incidental findings is staggering, but little information has been given in order to rule out potential malignant cases in symptomatic adult patients. Here, we describe a case of an anomalous course of the coronary artery with an acute (<45°) take-off angle, as well as an inter-arterial course between a dilated ascending aorta and a dilated pulmonary artery, and how we could manage this patient in our clinical practice.
Papillary Meningioma: Case Presentation with Emphasis on Surgical and Medical Therapy of a Rare Variant of Meningioma
Authors: Cazzato Gerardo , Internò Valeria , Cimmino Antonietta , Messina Raffaella , Tucci Marco , Lettini Teresa , Resta Leonardo , Ingravallo Giuseppe
Published: September 2021

Meningioma is one of the most frequent neoplasms of all in the central nervous system. Different variants are known, and of these some have peculiar characteristics, both from a morphological point of view and from a biological point of view. Here, we present a rare case of relapsed papillary meningioma in a young patient, focusing on histological characteristics, medical-surgical therapy and focusing on the risk of progression and/or recurrence of the lesion if not completely eradicated. Finally, we provide detailed molecular characteristics of the case in question.
Chinese University Students’ Experience of WeChat-Based English-Language Vocabulary Learning
Authors: Li Fan , Fan Si , Wang Yanjun , Lu Jinjin
Published: September 2021

The outbreak of COVID-19 worldwide in 2020 has posed tremendous challenges to higher education globally. Teaching English as a foreign language (TEFL) is among the many areas affected by the pandemic. The unexpected transition to online teaching has increased challenges for improving and/or retaining students’ language proficiency. WeChat, a popular social application in China, was widely used for TEFL at Chinese universities before COVID-19. However, it remains unclear whether the use of WeChat can facilitate Chinese university students’ English-language lexical proficiency during the pandemic. To fill this gap, the aim of the present study was two-fold: (1) it initially explored the relationship between the variables including students’ academic years, genders, and academic faculties/disciplines, and their lexical proficiency; and (2) it evaluated the effectiveness of a WeChat-assisted lexical learning (WALL) program in facilitating learning outcomes of English-language vocabulary. One hundred and thirty-three students at a university in Northern China participated in the WALL program for three weeks. As the results indicated, the independent variables had no correlation with the students’ lexical proficiency. More importantly, the students had a decline in the test scores after using the program, compared to their initial test scores. Moreover, the difference was reported to be medium. The findings further proposed questions on applying WeChat to vocabulary teaching in a large-scaled transition. The study is expected to provide insights for tertiary institutions, language practitioners, and student stakeholders to troubleshoot the potential problems regarding implementing WeChat-based TEFL pedagogies.
Identified Challenges from Faculty Teaching at Predominantly Undergraduate Institutions after Abrupt Transition to Emergency Remote Teaching during the COVID-19 Pandemic
Authors: Colclasure Blake C. , Marlier AnnMarie , Durham Mary F. , Brooks Tessa Durham , Kerr Mekenzie
Published: September 2021

COVID-19 has been one of the most significant disruptors of higher education in modern history. Higher education institutions rapidly transitioned to Emergency Remote Teaching (ERT) in mid-to-late March of 2020. The extent of COVID-19’s impact on teaching and learning, and the resulting challenges facilitating ERT during this time, likely varied by faculty, institutional, and geographical characteristics. In this study, we identified challenges in teaching and learning during the initial transition to ERT at Predominantly Undergraduate Institutions (PUIs) in the Midwest, United States. We conducted in-depth interviews with 14 faculty teaching at Midwestern PUIs to explore their lived experiences. We describe the most overarching challenges related to faculty teaching through four emergent themes: pedagogical changes, work-life balance, face-to-face interactions, and physical and mental health. Five themes emerged that we used to describe the most overarching challenges related to students and their learning: learning patterns, technology access, additional responsibilities, learning community, and mental health. Based upon the identified challenges, we provide broad recommendations that can be used to foster a more successful transition to ERT in unforeseen regional or global crises in the future.
Automatic Optimization of Gas Insulated Components Based on the Streamer Inception Criterion
Authors: Lucchini Francesco , Marconato Nicolò , Bettini Paolo
Published: September 2021

Gas insulated transmission lines (GILs) are used in electrical systems mainly for power transmission and High Voltage substation interconnection. In this paper, we focus on the development of complex numerical tools for the optimization of gas insulated HVDC components by the estimation of realistic electric field distribution and the voltage holding of the designed geometry. In particular, the paper aims at describing the correct modelling approach suitable to study high voltage components in DC, considering the nonlinear behaviour characterizing the electrical conductivity of solid and gas insulators. The simulated field distribution is then adopted to estimate the voltage holding of the dielectric gas, with a convenient engineering technique, based on the streamer criterion. These two tools are integrated in an automatic optimization package developed in COMSOL® and MATLAB®, with the purpose of adjusting the critical geometry features, suffering from excessive electrical stress and possibly giving rise to electrical breakdown, in order to guide the designer towards a robust solution.
A Study on Water-Induced Damage Severity on Diesel Engine Injection System Using Emulsified Diesel Fuels
Authors: Kim Min-Seop , Akpudo Ugochukwu Ejike , Hur Jang-Wook
Published: September 2021

Diesel engine emissions contribute nearly 30% of greenhouse effects and diverse health and environmental problems. Amidst these problems, it is estimated that there will be a 75% increase in energy demand for transportation by 2040, of which diesel fuel constitutes a major source of energy for transportation. Being a major source of air pollution, efforts are currently being made to curb the pollution spread. The use of water-in-diesel (W/D)-emulsified fuels comes as a readily available (and cost-effective) option with other benefits including engine thermal efficiency, reduced costs, and NOx reduction; nonetheless, the inherent effects—power loss, component wear, corrosion, etc. still pose strong concerns. This study investigates the behavior and damage severity of a common rail (CR) diesel fuel injection system using exploratory and statistical methods under different W/D emulsion conditions and engine speeds. Results reveal that the effect of W/D emulsion fuels on engine operating conditions are reflected in the CR, which provides a reliable avenue for condition monitoring. Also, the effect of W/D emulsion on injection system components-piston, nozzle needle, and ball seat–are presented alongside related discussions.
Investigation of Inertia Response and Rate of Change of Frequency in Low Rotational Inertial Scenario of Synchronous Dominated System
Authors: Gonzalez-Longatt Francisco , Roldan-Fernandez Juan Manuel , Chamorro Harold R. , Arnaltes Santiago , Rodriguez-Amenedo Jose Luis
Published: September 2021

The shift to a sustainable energy future is becoming more reliant on large-scale deployment of renewable and distributed energy resources raising concerns about frequency stability. Rate of Change of Frequency (RoCoF) is necessary as a system inertia metric in order for network operators to perform control steps to preserve system operation. This paper presents in a straightforward and illustrative way several relevant aspects of the inertia response and RoCoF calculation that could help to understand and explain the implementation and results of inertial response controllers on power converter-based technologies. Qualitative explanations based on illustrative numerical experiments are used to cover the effects on the system frequency response of reduced rotational inertia in synchronous dominated power systems. One main contribution of this paper is making evident the importance of the governor action to avoid the synchronous machine taking active power from the system during the recovering period of kinetic energy in an under frequency event.
Thermal Evaluation of a Micro-Coaxial Antenna Set to Treat Bone Tumors: Design, Parametric FEM Modeling and Evaluation in Multilayer Phantom and Ex Vivo Porcine Tissue
Authors: Ramírez-Guzmán Texar Javier , Trujillo-Romero Citlalli Jessica , Martínez-Valdez Raquel , Leija-Salas Lorenzo , Vera-Hernández Arturo , Rico-Martínez Genaro , Ortega-Palacios Rocío , Gutiérrez-Martínez Josefina
Published: September 2021

Bone cancer is rare in adults, the most affected persons by this disease are young people and children. The common treatments for bone cancer are surgery, chemotherapy, and targeted therapies; however, all of them have side-effects that decrease the patient’s quality of life. Thermotherapy is one of the most promising treatments for bone cancer; its main goal is to increase the tumor temperature to kill cancerous cells. Although some micro-coaxial antennas have been used to treat bone tumors, most of them are designed to treat soft tissue. Therefore, the purpose of this work is to analyze the thermal behavior of four micro-coaxial antennas specifically designed to generate thermal ablation in bone tissue to treat bone tumors, at 2.45 GHz. The proposed antennas were the metal-tip monopole (MTM), the choked metal-tip monopole (CMTM), the double slot (DS) and the choked double slot (CDS). The design and optimization of the antennas by using the Finite Element Method (FEM) allow to predict the optimal antenna dimensions and their performance when they are in contact with the affected biological tissues (bone, muscle, and fat). In the FEM model, a maximum power transmission was selected as the main parameter to choose the optimum antenna design, i.e., a Standing Wave Ratio (SWR) value around 1.2–1.5. The four optimized antennas were constructed and experimentally evaluated. The evaluation was carried out in multilayer phantoms (fat, muscle, cortical, and cancellous bone) and ex vivo porcine tissue at different insertion depths of the antennas. To fully evaluate the antennas performance, the standing wave ratio (SWR), power loss, temperature profiles, and thermal distributions were analyzed. In the experimentation, the four antennas were able to reach ablation temperatures (>60 °C) and the highest reached SWR was 1.7; the MTM (power loss around 16%) and the CDS (power loss around 6.4%) antennas presented the lowest SWR values depending on the antenna insertion depth, either in multilayer tissue phantom or in ex vivo tissue. These proposed antennas allow to obtain ablation temperatures with an input power of 5 W after 5 min of treatment; these values are lower than the ones reported in the literature.
Highly Stable Inverted CdSe/ZnS-Based Light-Emitting Diodes by Nonvacuum Technique ZTO as the Electron-Transport Layer
Authors: Hussain Sajid , Saeed Fawad , Raza Ahmad , Parveen Abida , Asghar Ali , Din Nasrud , Chao Zhang , Chen Jing , Khan Qasim , Lei Wei
Published: September 2021

CdSe/ZnS quantum dots (QDs) have attracted great consideration from investigators owing to their excellent photo-physical characteristics and application in quantum dot light-emitting diodes (QD-LEDs). The CdSe/ZnS-based inverted QD-LEDs structure uses high-quality semiconductors electron transport layers (ETLs), a multilayered hole transporting layers (HTLs). In QD-LED, designing a device structure with a minimum energy barrier between adjacent layers is very important to achieve high efficiency. A high mobility polymer of poly (9,9-dioctylfluorene-co-N-(4-(3-methylpropyl)) diphenylamine (TFB) was doped with 4,4′-bis-(carbazole-9-yl) biphenyl (CBP) with deep energy level to produce composite TFB:CBP holes to solve energy mismatch (HTL). In addition, we also improved the QD-LED device structure by using zinc tin oxide (ZTO) as ETL to improve device efficiency. The device turn-on voltage Vt (1 cd m−2) with ZTO ETL reduced from 2.4 V to 1.9 V significantly. Furthermore, invert structure devices exhibit luminance of 4296 cd m−2, current-efficiency (CE) of 7.36 cd A−1, and external-quantum efficiency (EQE) of 3.97%. For the QD-LED based on ZTO, the device efficiency is improved by 1.7 times.
Implementation of an Autonomous Overtaking System Based on Time to Lane Crossing Estimation and Model Predictive Control
Authors: Lin Yu-Chen , Lin Chun-Liang , Huang Shih-Ting , Kuo Cheng-Hsuan
Published: September 2021

According to statistics, the majority of accidents are attributed to driver negligence, especially when a driver intends to lane change or to overtake another vehicle, which is most likely to cause accidents. In addition, overtaking is one of the most difficult and complex functions for the development of autonomous driving technologies because of the dynamic and complicated task involved in the control strategy and electronic control systems, such as steering, throttle, and brake control. This paper proposes a safe overtaking maneuver procedure for an autonomous vehicle based on time to lane crossing (TLC) estimation and the model predictive control scheme. As overtaking is one of the most complex maneuvers that require both lane keeping and lane changing, a vision-based lane-detection system is used to estimate TLC to make a timely and accurate decision about whether to overtake or remain within the lane. Next, to maintain the minimal safe distance and to choose the best timing to overtake, the successive linearization-based model predictive control is employed to derive an optimal vehicle controller, such as throttle, brake, and steering angle control. Simultaneously, it can make certain that the longitudinal acceleration and steering velocity are maintained under constraints to maintain driving safety. Finally, the proposed system is validated by real-world experiments performed on a prototype electric golf cart and executed in real-time on the automotive embedded hardware with limited computational power. In addition, communication between the sensors and actuators as well as the vehicle control unit (VCU) are based on the controller area network (CAN) bus to realize vehicle control and data collection. The experiments demonstrate the ability of the proposed overtaking decision and control strategy to handle a variety of driving scenarios, including a lane-following function when a relative yaw angle exists and an overtaking function when the approaching vehicle has a different lateral velocity.