bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of an Incentive Programme for Increasing Green Infrastructure on Vineyards
Authors: Goodall Anna-Kate , McWilliam Wendy , Meurk Colin , Schelezki Olaf , Muangsri Suphicha
Published: September 2023

Wine grape ecosystems with low species richness and reliance on agrichemicals have weak resilience to environmental impacts. Increasing biodiversity through green infrastructure (GI) not only helps mitigate some of these impacts but can provide additional benefits to growers and the public. Despite this, many vineyards have limited GI. While scholars suggest incentive programmes may help to encourage GI implementation, few studies have evaluated their effectiveness. We surveyed winegrowers and their vineyards in the Waipara Valley sub-region, New Zealand, to evaluate an incentive programme aimed at increasing GI on vineyards, particularly indigenous vegetation. The results indicated the programme was effective in encouraging growers to plant indigenous plants in areas incapable or unsuitable for growing grapes, largely in support of nature conservation, aesthetics, branding, and sales. It was less successful in encouraging growers to plant them in productive areas. While substantial GI, primarily in the form of inter-row cover crops, was managed in these areas, most were exotic plants seen by growers to provide superior services (especially erosion control, weed suppression and pest regulation) at lower management complexity and cost. Growers identified six GI enablers: (1) promoting GI types that provide greater grower services than disservices and costs of implementation and management; (2) implementing GI where biophysical conditions support success; (3) providing assistance with plant selection and design; (4) providing GI implementation and/or management funding; (5) developing GI certification policies and regional association programmes; and (6) providing government GI regulations, strategies, and incentives. They also identified five barriers: (1) insufficient grower appreciation for indigenous GI services; (2) grower concerns that some GI disservices were greater than their services; (3) grower belief that costs of GI implementation and/or management were greater than those of alternative practices; (4) harsh and remote GI growing conditions; (5) lack of grower knowledge regarding how to design plantings, especially those that could provide multiple services; and (6) lack of sufficient financial resources for GI implementation and/or management. Twenty recommendations for improving GI implementation are provided.
The Interplay between the 1980 Hague Convention on the Civil Aspects of International Child Abduction and Domestic Violence
Authors: Trimmings Katarina , Momoh Onyója , Kalaitsoglou Konstantina
Published: September 2023

When a mother commits an international child abduction, even if she is fleeing domestic violence perpetrated by the left-behind father, she is bound to face complicated return proceedings under the 1980 Hague Child Abduction Convention. Such mothers are particularly vulnerable; apart from the costly, cross-border proceedings they face, if the court issues a return order, they risk returning to the abusive setting they fled from. This article explores avenues for safeguarding the protection of abducting mothers in return proceedings. The authors provide a range of potential avenues for improving the standing of the abducting mother fleeing domestic violence, including judicial and legislative interventions. The article delves deeper by considering the interplay between international child abduction law and international refugee law in cases involving domestic violence allegations. Particular emphasis is given to Article 20 and the growing instances of mothers defending return orders on asylum grounds pursuant to Article 20 and the flowing human rights implications. The authors point out a niche area for further research: the interplay between domestic violence and asylum claims.
Impact of IL-12B Genetic Variants on Antiviral Treatment Response among Hepatitis B Patients in Pakistan
Authors: Badshah Yasmin , Shabbir Maria , Zafar Sameen , Mussarat Uzma , Ikram Aamer , Javed Sumbal , Akhtar Hashaam
Published: September 2023

HBV is a continuous major global health concern. Genetic factors of hosts are known to play a role in HBV infection outcomes. This study aimed to reveal the association of IL-12b 3′ UTR variant rs3212227 in HBV patients. Genotyping was performed using ARMS-PCR to detect IL-12b rs3212227 polymorphism. The patients were categorized into groups based on their response to the antiviral therapy. Group I: non-sustained virological response (NSR); Group II: sustained virological responders (SVR); and Group III: HBV-positive fresh cases. ALT levels were measured to evaluate liver function, and viral load was determined to evaluate viral infectivity among the study groups. The variant genotype CC was found to be significantly associated with the non-sustained virological response to the antiviral therapy (with a p-value of 0.0117; OR = 2.914; RR = 1.556). It was also determined that the genotype CC was the most prevalent genotype among both genders in the NSR group. Viral load was found to be 6-fold higher in Group III compared to Group I and Group II. The results suggest that genotype CC is the most prevalent genotype in the NSR groups, and it is associated with a poor response to antiviral therapy in Pakistani patients with HBV infection.
Manipulate A2/B2 Structures in AlCrFexNi Alloys for Improved Mechanical Properties and Wear Resistance
Authors: Diao Guijiang , Wu Mingyu , He Anqiang , Xu Zhen , Mousavi Seyed Elias , Li Dongyang
Published: September 2023

Precipitation strengthening of body-center cubic (A2) alloys via ordered B2 nanoprecipitates is expected to achieve a desirable combination of strength and ductility. In this work, the A2/B2 configuration is manipulated by adjusting Fe content in medium-entropy AlCrFexNi (x = 0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0) alloys fabricated via arc-melting for improved mechanical properties and wear resistance. As Fe content increases, the fraction of A2 phase increases, and A2 nanoprecipitates in the B2 matrix change to a weave-like A2/B2 structure. Continuously increasing Fe content leads to a mixture of BMAP (B2 matrix with A2 precipitates) and AMBP (A2 matrix with B2 precipitates), and finally to a complete AMBP structure. The yield strength decreases and fracture strain increases with increasing Fe content except x = 0. The alloy of x = 0 displays slightly higher hardness because of its relatively brittle B2 matrix. Cracks tend to propagate along A2/B2 interfaces. AMBP structure exhibits greater toughness than the BMAP structure. The alloy of x = 0 displays the second-greatest wear volume loss due to its relatively brittle B2 matrix. When Fe is added, the wear volume loss decreases considerably but shows a trend of an upward parabola with respect to the Fe content. After achieving the highest volume loss at x = 1.5 with a mixture of AMBP and BMAP, the volume loss decreases again. A completely uniform AMBP structure at x = 3.0 shows the least volume loss.
Supramolecular Self-Assembly of the Zwitterionic Sn(IV)-Porphyrin Complex
Authors: Shee Nirmal Kumar , Kim Hee-Joon
Published: September 2023

[Sn(OSO3)2(TPyHP)](HSO4)2∙8H2O (1), an ionic Sn(IV)-porphyrin complex, was prepared by reacting [Sn(OH2)2TPyP] with dilute sulfuric acid. X-ray structural analysis revealed that the zwitterionic [Sn(OSO3)2TPyHP]2+ species consists of two anionic axial Sn–O–SO3 units and four peripheral pyridinium moieties, with an overall dicationic charge balanced by two hydrogen sulfate (HSO4−) counter-anions. Ionic hydrogen bonding between the oxygen atoms of axial sulfato ligands and the peripheral pyridinium groups of adjacent Sn(IV)-porphyrin cations led to the formation of a 1D channel filled with counter-anions and water molecules. The supramolecular self-assembly of 1 was further characterized using various spectroscopic techniques, including 1H NMR spectroscopy, elemental analysis, ESI-mass spectrometry, UV-vis spectroscopy, fluorescence spectroscopy, FT-IR spectroscopy, thermogravimetric analysis (TGA), and powder X-ray diffractometry. The zwitterionic [Sn(OSO3)2TPyHP]2+ complex is a structurally well-defined complementary scaffold involved in supramolecular self-assembly. This novel class of ion-assembled metalloporphyrin is a potential functional porphyrin material used in ion exchange applications.
Comparison of Sublimation 3D Scanning Sprays in Terms of Their Effect on the Resulting 3D Scan, Thickness, and Sublimation Time
Authors: Franke Jakub , Koutecký Tomáš , Koutný Daniel
Published: September 2023

This study compared eight sublimation scanning sprays in terms of their effect on 3D scanning results, coating thickness, and sublimation time. The work used an automated spraying system to ensure the same deposition conditions for all tested materials. All experiments were performed under the same environmental conditions to exclude the influence of the ambient environment on the coatings. All tested scanning sprays created coatings with thicknesses in the order of tens of micrometers that were detectable by the 3D scanner Atos III Triple Scan. The coatings must be applied carefully when accurate measurements are required. All used materials enabled the capture of the highly reflective surface of the Si-wafer. However, the differences between some sprays were significant. Sublimation time measurements showed that all coatings disappeared from the Si-wafer surface completely. Nevertheless, all coatings left visible traces on the mirror-like surface. They were easily wiped off with a cloth.
High-Modulus Laminated SiC/AZ91 Material with Adjustable Microstructure and Mechanical Properties Based on the Adjustment of the Densities of the Ceramic Layers
Authors: Du Zeqi , Deng Kunkun , Nie Kaibo , Wang Cuiju , Xu Chao , Shi Quanxin
Published: September 2023

To address the issue of inadequate strength and plasticity in magnesium matrix composites, SiC preforms were prepared using the freeze-casting process. The effects of sintering temperature on the microstructure, mechanical properties, and fracture behavior of SiCp/AZ91 magnesium matrix composites were studied by controlling the density of SiC preforms through low-temperature sintering. The results indicate that as the sintering temperature decreases, the reaction products in the SiC layer decrease, resulting in lower SiC preform density and increased content of AZ91 alloy filling in the layer. The increased alloy content in the ceramic layer not only inhibits crack initiation but also hinders crack propagation, thereby endowing the SiCp/AZ91 laminated material with excellent compressive strength and compressive strain. At the sintering temperature of 900 °C, the SiCp/AZ91 laminated material exhibits impressive compressive strength and strain values of 623 MPa and 8.77%, respectively, which demonstrates an excellent combination of strength and toughness.
Theoretical and Experimental Study of the Photocatalytic Properties of ZnO Semiconductor Nanoparticles Synthesized by Prosopis laevigata
Authors: Luque Morales Mizael , Luque Morales Priscy Alfredo , Chinchillas Chinchillas Manuel de Jesús , Orozco Carmona Víctor Manuel , Gómez Gutiérrez Claudia Mariana , Vilchis Nestor Alfredo Rafael , Villarreal Sánchez Rubén César
Published: September 2023

In this work, the photocatalytic activity of nanoparticles (NPs) of zinc oxide synthetized by Prosopis laevigata as a stabilizing agent was evaluated in the degradation of methylene blue (MB) dye under UV radiation. The theoretical study of the photocatalytic degradation process was carried out by a Langmuir–Hinshelwood–Hougen–Watson (LHHW) model. Zinc oxide nanoparticles were synthesized by varying the concentration of natural extract of Prosopis laevigata from 1, 2, and 4% (weight/volume), identifying the samples as ZnO_PL1%, ZnO_PL2%, and ZnO_PL4%, respectively. The characterization of the nanoparticles was carried out by Fourier transform infrared spectroscopy (FT-IR), where the absorption band for the Zn-O vibration at 400 cm−1 was presented; by ultraviolet–visible spectroscopy (UV–vis) the value of the band gap was calculated, resulting in 2.80, 2.74 and 2.63 eV for the samples ZnO_PL1%, ZnO_PL2%, and ZnO_PL4%, respectively; XRD analysis indicated that the nanoparticles have a hexagonal zincite crystal structure with an average crystal size of 55, 50, and 49 in the sample ZnO_PL1%, ZnO_PL2%, and ZnO_PL4%, respectively. The morphology observed by TEM showed that the nanoparticles had a hemispherical shape, and the ZnO_PL4% sample presented sizes ranging between 29 and 45 nm. The photocatalytic study showed a total degradation of the MB in 150, 120, and 60 min for the samples ZnO_PL1%, ZnO_PL2%, and ZnO_PL4%, respectively. Also, the model explains the experimental observation of the first-order kinetic model in the limit of low concentrations of dye, indicating the influence of the mass transfer processes.
The Prevalence of Elder Abuse and its Association with Frailty in Elderly Patients at the Outpatient Department of a Super-Tertiary Care Hospital in Northern Thailand
Authors: Choksomngam Yanee , Petrungjarern Terdsak , Ketkit Perapoln , Boontak Pakpoom , Panya Ratchanon , Wongpakaran Tinakon , Wongpakaran Nahathai , Lerttrakarnnon Peerasak
Published: September 2023

Background and Objectives: The global population is undergoing rapid aging, resulting in an increase in geriatric syndromes and hidden health issues such as elder abuse. However, the prevalence of elder abuse varies across different settings. The objective of this study is to determine the prevalence and factors associated with elder abuse at the outpatient department of a super-tertiary care hospital in northern Thailand. Materials and Methods: This cross-sectional study involved 210 elderly patients who visited Maharaj Nakorn Chiang Mai Hospital between May and August 2022. The participants completed several assessments, including the Mini-Cog, Thai Geriatric Depression Scale-6, Core Symptom Index-15, FRAIL scale, Barthel Activities of Daily Living, Interview Guideline for Screening for Elder Abuse, and Diagnostic Criteria for Elder Abuse. Fisher’s exact test was used to examine the association between the factors and elder abuse. Results: The Screening for Elder Abuse yielded noteworthy results, with 15.7% of the elderly patients having experienced psychological abuse. However, only a smaller subset of study participants, comprising five individuals or 2.38%, met the diagnostic criteria for elder abuse. Furthermore, statistical analysis revealed no significant correlation between elder abuse and the other factors examined in the study. Conclusions: As a result, it is crucial for hospitals to consider preventive measures and implement routine screening protocols.
Fabrication and Characterization of Dielectric ZnCr2O4 Nanopowders and Thin Films for Parallel-Plate Capacitor Applications
Authors: Mykhailovych Vasyl , Caruntu Gabriel , Graur Adrian , Mykhailovych Mariia , Fochuk Petro , Fodchuk Igor , Rotaru Gelu-Marius , Rotaru Aurelian
Published: September 2023

We report here the successful shape-controlled synthesis of dielectric spinel-type ZnCr2O4 nanoparticles by using a simple sol-gel auto-combustion method followed by successive heat treatment steps of the resulting powders at temperatures from 500 to 900 °C and from 5 to 11 h, in air. A systematic study of the dependence of the morphology of the nanoparticles on the annealing time and temperature was performed by using field effect scanning electron microscopy (FE-SEM), powder X-ray diffraction (PXRD) and structure refinement by the Rietveld method, dynamic lattice analysis and broadband dielectric spectrometry, respectively. It was observed for the first time that when the aerobic post-synthesis heat treatment temperature increases progressively from 500 to 900 °C, the ZnCr2O4 nanoparticles: (i) increase in size from 10 to 350 nm and (ii) develop well-defined facets, changing their shape from shapeless to truncated octahedrons and eventually pseudo-octahedra. The samples were found to exhibit high dielectric constant values and low dielectric losses with the best dielectric performance characteristics displayed by the 350 nm pseudo-octahedral nanoparticles whose permittivity reaches a value of ε = 1500 and a dielectric loss tan δ = 5 × 10−4 at a frequency of 1 Hz. Nanoparticulate ZnCr2O4-based thin films with a thickness varying from 0.5 to 2 μm were fabricated by the drop-casting method and subsequently incorporated into planar capacitors whose dielectric performance was characterized. This study undoubtedly shows that the dielectric properties of nanostructured zinc chromite powders can be engineered by the rational control of their morphology upon the variation of the post-synthesis heat treatment process.
Trichoderma Species Problematic to the Commercial Production of Pleurotus in Italy: Characterization, Identification, and Methods of Control
Authors: Lombardi Nadia , Pironti Angela , Manganiello Gelsomina , Marra Roberta , Vinale Francesco , Vitale Stefania , Lorito Matteo , Woo Sheridan Lois
Published: September 2023

Nine isolates of Trichoderma were obtained from the diverse phases of compost preparation and the production of different commercial varieties of oyster mushrooms Pleurotus spp. with the apparent presence of green mould disease. The isolates were morphologically and genetically characterized. Molecular fingerprinting indicated that the isolates belonged to the species T. pleuroticola and T. harzianum. In order to identify control measures, changes in temperature, pH, and application of fungicides were tested on the present isolates, using known Trichoderma biocontrol strains as a reference. Fungicide effects on the growth of Pleurotus isolates were also assessed. The optimal growth temperatures were 25 °C for Trichoderma isolates and 28 °C for Pleurotus isolates, and Trichoderma always grew faster than Pleurotus. In particular, a reduction of about 30% was recorded for some of the Trichoderma isolates when comparing the colony growth at 25 and 28 °C. Trichoderma isolates developed well within a wide pH range, with the best growth occurring between pH 5 and 7, whereas Pleurotus preferred more alkaline conditions (pH 8 to 9). Prochloraz and metrafenone were found to inhibit Trichoderma growth with different dose responses that did not affect the growth of Pleurotus spp. In particular, metrafenone was the most effective active ingredient that inhibited the majority of the Trichoderma isolates (1–25% growth reduction).
In Vivo, In Vitro and In Silico Study of Cucurbita moschata Flower Extract: A Promising Source of Natural Analgesic, Anti-Inflammatory, and Antibacterial Agents
Authors: Hossain Md. Rabiul , Alam Rashedul , Chung Hea-Jong , Eva Taslima Akter , Kabir Mohammed Fazlul , Mamurat Husnum , Hong Seong-Tshool , Hafiz Md. Al , Hossen S. M. Moazzem
Published: September 2023

For thousands of years, medicinal plants have played a pivotal role in maintaining human health and improving the quality of human life. This study was designed to analyze the analgesic, anti-inflammatory, and antibacterial potentials of a hydro-methanolic extract of Cucurbita moschata flowers, along with qualitative and quantitative phytochemical screening. The anti-inflammatory effect was tested using the in vitro membrane stabilizing method for human red blood cells (HRBC), the analgesic effect was tested using the in vivo acetic acid-induced writing method, and the antibacterial effect was tested using the disc diffusion method. In silico ADME/T and molecular docking studies were performed to assess the potential of the stated phytochemicals against Cyclooxygenase-II enzyme. Phytochemical screening confirmed the presence of flavonoids, alkaloids, glycosides, tannins, and carbohydrates. The flower extract demonstrated the maximum protection of human red blood cells at 1000 µg/mL, with a 65.73% reduction in hemolysis in a hypotonic solution. The extract also showed significant (p < 0.05) and dose-dependent analgesic effects at oral doses of 200 and 400 mg/kg on the tested animals. Furthermore, the flower extract exhibited potent antibacterial activity due to the disc diffusion method, which was compared with standard ciprofloxacin. In silico testing revealed that 42 phytochemicals exhibited notable pharmacokinetic properties and passed drug likeness screening tests. Among the six best-selected compounds, 3,4-dihydro-2H-pyran-2-yl)methanamine showed the highest binding affinity (−10.1) with significant non-bonding interactions with the target enzyme. In conclusion, the hydro-methanolic extract of Cucurbita moschata was found to be rich in various phytochemicals that may be associated with therapeutic potential, and this study supports the traditional use of Cucurbita moschata flowers in the management of inflammation and painful conditions.
Making Every Single Puff Count—Simple and Sensitive E-Cigarette Aerosol Sampling for GCxIMS and GC-MS Analysis
Authors: Augustini Alexander L. R. M. , Borg Christopher , Sielemann Stefanie , Telgheder Ursula
Published: September 2023

The analysis of the aerosol from tobaccoless electronic cigarettes (e-cigarettes) is an important part of understanding their impact on human health, yet sampling aerosol from e-cigarettes is still considered a challenge. It lacks a standard method for research and quality control and there are a variety of methods. However, few are simple and inexpensive, and none have been suggested for the use with gas chromatography coupled ion mobility spectrometry (GCxIMS). This work presents and evaluates such a setup made from standard lab equipment to quickly collect a quantitative sample from the aerosol of a single puff (5 s totaling 125 mL). The aerosol condensates directly in the cooled headspace (HS) vial, which is analyzed in the HS-GCxIMS or mass spectrometer (HS-GC-MS). The combined use of GC-MS and GCxIMS allows the simple and sensitive identification of unknown substances in complex mixtures and the identification of degradation products in the aerosols. A calibration of 26 flavor compounds (0.2–20 µg/g) was created using single puffs of a spiked, flavorless commercial refill solution and 2-alkanones as internal standards. This sensitive but easily reproducible setup enables a wide range of further investigations, even for labs that were previously unable to afford it.
Expert Consensus on the Long-Term Effectiveness of Medical Nutrition Therapy and Its Impact on the Outcomes of Adults with Phenylketonuria
Authors: Rocha Júlio César , Ahring Kirsten K. , Bausell Heather , Bilder Deborah A. , Harding Cary O. , Inwood Anita , Longo Nicola , Muntau Ania C. , Pessoa André L. Santos , Rohr Fran , Sivri Serap , Hermida Álvaro
Published: September 2023

Many adults with phenylketonuria (PKU) rely on medical nutrition therapy (MNT; low phenylalanine (Phe) diet with protein substitutes/medical foods) to maintain blood Phe concentrations within recommended ranges and prevent PKU-associated comorbidities. Despite disease detection through newborn screening and introduction of MNT as early as birth, adherence to MNT often deteriorates from childhood onwards, complicating the assessment of its effectiveness in the long term. Via a modified Delphi process, consensus (≥70% agreement) was sought on 19 statements among an international, multidisciplinary 13-member expert panel. After three iterative voting rounds, the panel achieved consensus on 17 statements related to the limitations of the long-term effectiveness of MNT (7), the burden of long-term reliance on MNT (4), and its potential long-term detrimental health effects (6). According to the expert panel, the effectiveness of MNT is limited in the long term, is associated with a high treatment burden, and demonstrates that adults with PKU are often unable to achieve metabolic control through dietary management alone, creating an unmet need in the adult PKU population.
Influence of Occupational Stress on the Body Mass Index of Hospital Workers: A Systematic Review
Authors: de Lira Carlos Rodrigo Nascimento , Akutsu Rita de Cássia , Coelho Lorene Gonçalves , da Silva Karine Brito Beck , Pitangueira Jacqueline Costa Dias , Zandonadi Renata Puppin , Costa Priscila Ribas de Farias
Published: September 2023

This systematic review aimed to identify the influence of occupational stress on the body mass index of hospital workers. After registering the protocol at PROSPERO (CRD42022331846), we started this systematic review following a search in seven databases, gray literature, as well as manual search and contact with specialists. The selection of studies was performed independently by two evaluators following the inclusion criteria: observational studies evaluating adult hospital workers, in which occupational stress was considered exposure and body composition as a result. The risk of bias in the included studies was assessed using the Joanna Briggs Institute Critical Appraisal checklist. We used the Grading of Recommendations Assessment, Development and Evaluation to grade the certainty of the evidence. Qualitative results were presented and synthesized through a qualitative approach, with simplified information in a narrative form. A total of 12 studies met the eligibility criteria and were included. This review comprised 10,885 workers (2312 men; 1582 women; and 6991 workers whose gender was not identified). Ten studies were carried out only with health workers, and two included workers from other sectors besides health workers. This review showed a relationship between occupational stress and changes in body mass index in hospital workers. However, most studies presented a moderate or high risk of bias and low quality of the evidence. These findings can be useful for clinical practice, administrators and leaders and provide insights for future research in the field of worker health in the hospital setting.
Household Food Security and Consumption of Sugar-Sweetened Beverages among New York City (NYC) Children: A Cross-Sectional Analysis of 2017 NYC Kids’ Data
Authors: Flórez Karen R. , Albrecht Sandra S. , Hwang Neil , Chambers Earle , Li Yan , Gany Francesca M. , Davila Marivel
Published: September 2023

Food insecurity is a stressor associated with adverse health outcomes, including the consumption of sugar-sweetened beverages (SSBs). Our study tests the hypothesis that other socioeconomic vulnerabilities may magnify this effect using cross-sectional data from the 2017 New York City (NYC) Kids Survey. Households providing an affirmative response to one or both food security screener questions developed by the US Department of Agriculture were coded as households with low food security. The number of sodas plus other SSBs consumed was standardized per day and categorized as 1 = none, 2 = less than one, and 3 = one or more. We tested the joint effect of low food security with chronic hardship, receipt of federal aid, and immigrant head of household on a sample of n = 2362 kids attending kindergarten and beyond using ordinal logistic regression and accounting for the complex survey design. Only having a US-born parent substantially magnified the effect of low household food security on SSB consumption (OR = 4.2, 95%CI: 2.9–6.3, p < 0.001) compared to the reference group of high household food security with an immigrant parent. The effect of low food security on SSB consumption among NYC children warrants intersectional approaches, especially to elucidate US-based SSB norms in low-food-security settings.
Research in the Field of Drug Design and Development
Authors: Biala Grazyna , Kedzierska Ewa , Kruk-Slomka Marta , Orzelska-Gorka Jolanta , Hmaidan Sara , Skrok Aleksandra , Kaminski Jakub , Havrankova Eva , Nadaska Dominika , Malik Ivan
Published: September 2023

The processes used by academic and industrial scientists to discover new drugs have recently experienced a true renaissance, with many new and exciting techniques being developed over the past 5–10 years alone. Drug design and discovery, and the search for new safe and well-tolerated compounds, as well as the ineffectiveness of existing therapies, and society’s insufficient knowledge concerning the prophylactics and pharmacotherapy of the most common diseases today, comprise a serious challenge. This can influence not only the quality of human life, but also the health of whole societies, which became evident during the COVID-19 pandemic. In general, the process of drug development consists of three main stages: drug discovery, preclinical development using cell-based and animal models/tests, clinical trials on humans and, finally, forward moving toward the step of obtaining regulatory approval, in order to market the potential drug. In this review, we will attempt to outline the first three most important consecutive phases in drug design and development, based on the experience of three cooperating and complementary academic centers of the Visegrád group; i.e., Medical University of Lublin, Poland, Masaryk University of Brno, Czech Republic, and Comenius University Bratislava, Slovak Republic.
Chitosan/Solid-Lipid Nanoparticles Hybrid Gels for Vaginal Delivery of Estradiol for Management of Vaginal Menopausal Symptoms
Authors: Abou-Taleb Heba A. , Fathalla Zeinab , Naguib Demiana M. , Fatease Adel Al , Abdelkader Hamdy
Published: September 2023

Hormonal replacement therapy is the mainstay treatment to improve quality of life and reduce mortality. With the increasing number of young women with early menopause, women now live longer (increased life expectancy). However, poor patient compliance with oral estrogen therapy has emerged. Intravaginal estrogen therapy can provide significant benefits with minimal risk for postmenopausal women with symptoms of the lower urinary tract and vaginal area but who do not want to take oral estrogen. In this study, estradiol-loaded solid lipid nanoparticles (SLPs) were prepared from compritol ATO 888 and precirol ATO 5, and two different stabilizers (Pluronic F127 and Tween 80) were studied. Selected SLPs (F3 and F6) were coated with different concentrations of the mucoadhesive and sustained-release polymer chitosan. Furthermore, gelation time, viscosity, mucoadhesion, ex vivo permeation, and in vitro irritation for vaginal irritation were studied. Particle sizes ranged between 450–850 nm, and EE% recorded 50–83% for the six SLPs depending on the type and amount of lipids used. Cumulative % drug release was significantly enhanced and was recorded at 51% to 83%, compared to that (less than 20%) for the control suspension of estradiol. Furthermore, extensive thermal gelation and mucoadhesion were recorded for chitosan-coated SLPs. Up to 2.2-fold increases in the permeation parameters for SLPs gels compared to the control suspension gel were recorded, revealing a slight to moderate irritation on Hela cell lines. These findings demonstrated chitosan-coated estradiol SLPs as novel and promising vaginal mucoadhesive hybrid nanogels.
Special Issue “Delivery Systems of Peptides and Proteins: Challenges, Status Quo and Future Perspectives”
Authors: Liu Wenjuan , He Haisheng , Qi Jianping
Published: September 2023

Peptides and proteins have emerged as more important therapeutic molecules compared to small molecular chemicals due to their high specificity and efficacy and low toxicity [...]
Revisiting p38 Mitogen-Activated Protein Kinases (MAPK) in Inflammatory Arthritis: A Narrative of the Emergence of MAPK-Activated Protein Kinase Inhibitors (MK2i)
Authors: Ganguly Payal , Macleod Tom , Wong Chi , Harland Mark , McGonagle Dennis
Published: September 2023

The p38 mitogen-activated protein kinase (p38-MAPK) is a crucial signaling pathway closely involved in several physiological and cellular functions, including cell cycle, apoptosis, gene expression, and responses to stress stimuli. It also plays a central role in inflammation and immunity. Owing to disparate p38-MAPK functions, it has thus far formed an elusive drug target with failed clinical trials in inflammatory diseases due to challenges including hepatotoxicity, cardiac toxicity, lack of efficacy, and tachyphylaxis, which is a brief initial improvement with rapid disease rebound. To overcome these limitations, downstream antagonism of the p38 pathway with a MAPK-activated protein kinase (MAPKAPK, also known as MK2) blockade has demonstrated the potential to abrogate inflammation without the prior recognized toxicities. Such MK2 inhibition (MK2i) is associated with robust suppression of key pro-inflammatory cytokines, including TNFα and IL-6 and others in experimental systems and in vitro. Considering this recent evidence regarding MK2i in inflammatory arthritis, we revisit the p38-MAPK pathway and discuss the literature encompassing the challenges of p38 inhibitors with a focus on this pathway. We then highlight how novel MK2i strategies, although encouraging in the pre-clinical arena, may either show evidence for efficacy or the lack of efficacy in emergent human trials data from different disease settings.
Coal and Gas Outburst Prediction Model Based on Miceforest Filling and PHHO–KELM
Authors: Shao Liangshan , Chen Wenjing
Published: September 2023

Coal and gas outbursts are some of the most serious coal mine disasters, and effective prediction of coal and gas outbursts can reduce the likelihood of accidents and fatalities. Previously conducted studies have established that machine learning has achieved results in the prediction of coal and gas outbursts, but there is a problem that the available accident data of coal and gas outbursts are diminished or missing. This paper proposes a prediction model based on multiple filling of chained equations for random forests (miceforest) and the Harris Hawk optimization algorithm with Piecewise chaos mapping (PHHO) to optimize the kernel extreme learning machine (KELM) to solve the problem of missing data in coal and gas outburst prediction and to improve prediction accuracy in the case of missing data. Firstly, the miceforest algorithm was adopted to fill missing values in the salient samples, and then the PHHO algorithm was used to optimize the parameters of KELM. Finally, the datasets before and after filling were input into the PHHO–KELM model for experimentation and comparison with other models. The results show that miceforest filling is effective in improving the salient sample accuracy and overall accuracy of predictions, but the improvement is not significant for non-salient samples. The use of the PHHO–KELM model can effectively avoid falling into a local optimum and further improve the prediction accuracy of the KELM algorithm. The salient sample accuracy and overall accuracy of the miceforest–PHHO–KELM model prediction are 96.77% and 98.50%. And an effective coal and gas outburst model has been proposed, which is the miceforest–PHHO–KELM model.
Recent Developments in In Vitro Spermatogenesis and Future Directions
Authors: Cho In Ki , Easley Charles A.
Published: September 2023

Recent developments in stem cell technologies have made significant advancements in the field of in vitro gametogenesis. In vitro gametogenesis (IVG) is a promising technology where functional gametes (sperm or egg cells) can be generated from stem cells. Scientists have made continuous advancements in the field and successfully derived fully functional sperm from stem cells in mice. Two recent papers generated excitement in IVG by generating bi-maternal and bi-paternal mice from embryonic stem cells (ESCs) and pluripotent stem cells (PSCs). IVG is a promising technology with potential applications that include infertility treatment, fertility preservation, same-sex reproduction, bypassing oocyte depletion in women with advanced age, conservation biology, genetic disorder prevention, and research into human germ cell development. In vitro spermatogenesis (IVS) is the attempt to recreate the process of spermatogenesis in a culture system. Spermatogenesis is essential for male fertility and reproductive health, but it can be impaired by various factors such as genetic defects, environmental toxicants, infections, aging, or medical therapies. Spermatogenesis is a complex and highly regulated process involving multiple cell proliferation, differentiation, and maturation stages. The main challenges of IVS are to provide a suitable microenvironment that mimics the testis in vivo, to support the survival and development of all the cell types involved in spermatogenesis, and to achieve complete and functional spermatogenesis. Therefore, there is a great interest in developing methods to study spermatogenesis in vitro, both for basic research and clinical applications. This review covers recent developments in in vitro spermatogenesis in the past two years. Advances in tissue engineering and regenerative medicine have introduced techniques like ex vivo tissue culture and technologies such as bioreactors, microfluidic systems, and organoids. Bioreactors and microfluidic systems replicate physiological conditions for tissue and cell cultivation, while organoids model organ functionality. Meanwhile, scaffolds, made from various materials, provide essential structural support, guiding the growth and organization of cells into functional tissues.
A Novel Error Sensitivity Analysis Method for a Parallel Spindle Head
Authors: Wang Liping , Li Mengyu , Yu Guang
Published: September 2023

Geometric errors are the main factors affecting the output accuracy of the parallel spindle head, and it is necessary to perform a sensitivity analysis to extract the critical geometric errors. The traditional sensitivity analysis method analyzes the output position and orientation errors independently, defining multiple sensitivity indices and making it difficult to determine critical geometric errors. In this paper, we propose sensitivity indices that can comprehensively consider position and orientation errors. First, the configuration of the hybrid machine tool is introduced, and the TCP position error model is derived. Then, the tool radius and the effective cutting length are introduced, and the sensitivity indices are defined. After that, the sensitivity analysis of the 3-DOF parallel spindle head is performed using the proposed sensitivity indices, and six critical geometric errors are extracted. The machining accuracy of the parallel spindle head can be greatly improved by improving the critical geometric errors. The proposed sensitivity analysis method can provide important guidance for machine tool accuracy design.
SDRSwin: A Residual Swin Transformer Network with Saliency Detection for Infrared and Visible Image Fusion
Authors: Li Shengshi , Wang Guanjun , Zhang Hui , Zou Yonghua
Published: September 2023

Infrared and visible image fusion is a solution that generates an information-rich individual image with different modal information by fusing images obtained from various sensors. Salient detection can better emphasize the targets of concern. We propose a residual Swin Transformer fusion network based on saliency detection, termed SDRSwin, aiming to highlight the salient thermal targets in the infrared image while maintaining the texture details in the visible image. The SDRSwin network is trained with a two-stage training approach. In the first stage, we train an encoder–decoder network based on residual Swin Transformers to achieve powerful feature extraction and reconstruction capabilities. In the second stage, we develop a novel salient loss function to guide the network to fuse the salient targets in the infrared image and the background detail regions in the visible image. The extensive results indicate that our method has abundant texture details with clear bright infrared targets and achieves a better performance than the twenty-one state-of-the-art methods in both subjective and objective evaluation.
Trend of Changes in Phenological Components of Iran’s Vegetation Using Satellite Observations
Authors: Khormizi Hadi Zare , Malamiri Hamid Reza Ghafarian , Kalantari Zahra , Ferreira Carla Sofia Santos
Published: September 2023

Investigating vegetation changes, especially plant phenology, can yield valuable information about global warming and climate change. Time series satellite observations and remote sensing methods offer a great source of information on distinctions and changing aspects of vegetation. The current study aimed to determine the trend and rate of changes in some phenological components of Iran’s vegetation. In this regard, the current study employed the daily NDVI (Normalized Difference Vegetation Index) product of the AVHRR sensor with a spatial resolution of 0.05° × 0.05°, named AVH13C1. Then, using the HANTS algorithm, images of amplitude zero, annual amplitude, and annual phase were prepared annually from 1982 to 2019. Using TIMESAT software, the starting, end, and length of time of growing season were calculated for each pixel time series to prepare annual maps. The Mann–Kendall statistical test was used to investigate the significance of changes during the study period. On average in the entire area of Iran, the annual phase was declining with a trend of −0.6° per year, and the time for the start and end of the season was declining by −0.3 and −0.65 days per year, respectively. Major changes were noticed in the northeast, west, and northwest regions of Iran, where the annual phase declined with a trend of −0.9° per year. Since the annual growth cycle of the plant (equivalent to 356 days) was in the form of a sinusoidal signal, and the angular changes in the sine wave were between zero and 360°, each degree of change was equivalent to 1.01 days per year. Therefore, the reduction in the annual phase by −0.9 degrees almost means a change in the time (due to the earlier negative start phase) of the start of the annual growth signal by −0.9 days per year. The time of the start and end of the growing season declined by −0.6 and −1.33 days per year, respectively. The reduction in annual phase and differences in time of the starting season from 1982 to 2019 indicate the acceleration and earlier initiation of various phenological processes in the area.
Spatiotemporal Change in Evapotranspiration across the Indus River Basin Detected by Combining GRACE/GRACE-FO and Swarm Observations
Authors: Cui Lilu , Yin Maoqiao , Zou Zhengbo , Yao Chaolong , Xu Chuang , Li Yu , Mao Yiru
Published: September 2023

Evapotranspiration (ET) is an important approach for enabling water and energy exchange between the atmosphere and the land, and it has a very close relationship with terrestrial water resources and the ecological environment. Therefore, it is of great scientific to accurately quantify the spatiotemporal change in ET and its impact factors to understand the terrestrial water change pattern, maintaining water resource security and protecting the ecological environment. Our goal is to study the spatiotemporal characteristics of ET in the Indus River basin (IRB) and their driving factors. In our study, we first integrated the multi-source satellite gravimetry observations using the generalized three-cornered hat and least square methods to obtain the high-precision and continuous spatiotemporal evolution features of ET in the IRB from 2003 to 2021. Finally, we combined nine hydrometeorological and land cover type data to analyze the factors influencing ET. The results indicate that the algorithm used in our study can improve the ET accuracy by 40%. During the study period, ET shows a significant increasing trend (0.64 ± 0.73 mm/a), and the increasing rate presents spatial distribution characteristics of high variability in the northern areas and low variability in the southern areas of the study region. ET has a close relationship with precipitation, specific humidity, total canopy water storage, surface temperature and wind speed (with a correlation coefficients greater than 0.53 and variable importance of projection greater than 0.84). Among these factors, precipitation, specific humidity and surface temperature have significant correlations with ET (correlation coefficients greater than 0.85 and variable importance of projection greater than 1.42). And wind speed has a more significant positive effect on ET in the densely vegetated regions. The impacts of climate change on ET are significantly greater than those of land cover types, especially for similar land cover types. Ice and snow are significantly different to other land cover types. In this region, ET is only significantly correlated with precipitation, specific humidity and snow water equivalent (variable importance of projection greater than 0.81), and the impacts of precipitation and specific humidity on ET have been significantly weakened, while that of snow water equivalent is significantly enhanced. Our results contribute to furthering the understanding of the terrestrial water cycle in subtropical regions.
The Classification of Hyperspectral Images: A Double-Branch Multi-Scale Residual Network
Authors: Fu Laiying , Chen Xiaoyong , Pirasteh Saied , Xu Yanan
Published: September 2023

With the continuous advancement of deep learning technology, researchers have made further progress in the hyperspectral image (HSI) classification domain. We propose a double-branch multi-scale residual network (DBMSRN) framework for HSI classification to improve classification accuracy and reduce the number of required training samples. The DBMSRN consists of two branches designed to extract spectral and spatial features from the HSI. Thus, to obtain more comprehensive feature information, we extracted additional local and global features at different scales by expanding the network width. Moreover, we also increased the network depth to capture deeper feature information. Based on this concept, we devise spectral multi-scale residuals and spatial multi-scale residuals within a double-branch architecture. Additionally, skip connections are employed to augment the context information of the network. We demonstrate that the proposed framework effectively enhances classification accuracy in scenarios with limited training samples through experimental analysis. The proposed framework achieves an overall accuracy of 98.67%, 98.09%, and 96.76% on the Pavia University (PU), Kennedy Space Center (KSC), and Indian Pines (IP) datasets, respectively, surpassing the classification accuracy of existing advanced frameworks under identical conditions.
Assessing Shoreline Changes in Fringing Salt Marshes from Satellite Remote Sensing Data
Authors: Castro Inês J. , Dias João M. , Lopes Carina L.
Published: September 2023

Salt marshes are highly important wetlands; however, external pressures are causing their widespread deterioration and loss. Continuous monitoring of their extent is paramount for the preservation and recovery of deteriorated and threatened salt marshes. In general, moderate-resolution satellite remote sensing data allow for the accurate detection of salt marsh shorelines; however, their detection in narrow and fringing salt marshes remains challenging. This study aims to evaluate the ability of Landsat-5 (TM), Landsat-7 (ETM+), and Sentinel-2 (MSI) data to be used to accurately determine the shoreline of narrow and fringing salt marshes, focusing on three regions of the Aveiro lagoon in Mira, Ílhavo and S. Jacinto channels. Shorelines were determined considering the Normalized Difference Vegetation Index (NDVI), and the accuracy of this methodology was evaluated against reference shorelines by computing the Root Mean Square Error (RMSE). Once validated, the method was used to determine historical salt marsh shorelines, and rates of change between 1984 and 2022 were quantified and analyzed in the three locations. Results evidence that the 30 m resolution Landsat data accurately describe the salt marsh shoreline (RMSE~15 m) and that the accuracy is maintained when increasing the spatial resolution through pan-sharpening or when using 10 m resolution Sentinel-2 (MSI) data. These also show that the salt marshes of the Ílhavo and S. Jacinto channels evolved similarly, with salt marsh shoreline stability before 2000 followed by retreats after this year. At the end of the four decades of study, an average retreat of 66.23 ± 1.03 m and 46.62 ± 0.83 m was found, respectively. In contrast to these salt marshes and to the expected evolution, the salt marsh of the Mira Channel showed retreats before 2000, followed by similar progressions after this year, resulting in an average 2.33 ± 1.18 m advance until 2022.