bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of the Structure of Humic Substances in Increasing Bacterial Survival
Authors: YA Nikolaev
Published: November 2020

Humic substances (HSs) are important for the functioning and stability of soil ecosystems; their physical and chemical properties, as well as their influence on plants and animals are well studied. However, the role of HSs in the physiology of microorganisms, in particular, in their survival under unfavorable conditions, is understood insufficiently. To evaluate the role of various chemical groups and physicochemical properties of HSs in the survival of soil bacteria, experiments were performed using eight HS preparations with different structure and properties. Addition of HSs to cultures of hydrocarbonoxidizing soil bacteria Rhodococcus erythropolis and Pseudomonas extremaustralis increased the titers of viable cells after 30 days of storage up to 10 times. This biological effect of HSs is due to their ability to stimulate the formation of stress-resistant persister cells. Correlation analysis established a relationship between the biological activity of HS and the atomic content of hydrogen and carbon, hydrophobicity, and the average molecular weight of HS. The atomic content of oxygen, as well as the content of CHn groups and various oxygen-containing groups (CH3O, CH2O, CHO, C=O, Alk-O) correlated with a negative effect of HS on bacterial viability. The content of aromatic groups and antioxidant capacity correlated with both activating and inhibiting biological activity of HSs. The stabilizing effect of HSs on bacterial cells is explained by their antioxidant activity,as well as by their ability to serve as a carbon source and to act as a terminal electron acceptor in the absence of oxygen. The obtained results elucidate an important function of HSs: to ensure long-term survival of bacteria in soil ecosystems in the periods between their active development.
Prevalence and Risk Factors of Pregnancy Associated Malaria in Pregnant Women Attending a General Hospital
Authors: UN Valentine
Published: November 2020

Malaria in pregnancy poses a serious public health issues especially in developing countries. This study was to determine the Prevalence and risk factors of Malaria among Pregnant women attending antenatal clinic in Aboh Mbaise General Hospital. A total of 284 pregnant women participated in this study. A structured interviewer-administered questionnaire was used to obtain some socio-demographic characteristics of patients. Thick and thin films were made from blood collected from each pregnant woman. The films were stained using the gold standard staining technique, Giemsa staining for detecting malaria parasites in blood. Data generated was analyzed using SPSS 23.0 statistical package. A p-value<0.05 was considered significant.The prevalence of malaria parasite among the pregnant women in Aboh Mbaise was 70.8%. Plasmodium falciparum was the only specie of malaria parasite found in this study. The age group 15 – 25 years, illiterates, unemployed and single patients had the highest prevalence of malaria positive (MP) patients of 81(28.5%), 80(28.2%), 140(49.3%) and 53(18.7%) respectively. Primigravidae had the highest frequency of MP patients of 109(38.4%) followed by multigravidae 92(32.4%) but there was no statistical significance (p>0.05). Malaria prevalence followed the trend of 1st trimester 80(28.2%) to 2nd trimester 66(23.2%) to 3rd trimester 45(15.8%) in that order but the differences were not statistically significant (p>0.05). The lowest frequency occurred in the month of February 9(3.2%) while the highest frequency occurred in the month of July 62(21.8%) but the difference was not statistically significant (p>0.05). Patients who had bushes around their compound, and who didn’t use mosquito bed net (MBN) and insecticide treated nets (ITNs); those patients surrounded by mosquito breeding sites and who didn’t use Intermittent Preventive Treatment (IPT) were significantly associated with malaria parasite infection (p< 0.05). The prevalence of malaria parasite among the pregnant women in was 70.8%. Lack of education and employment; and risk factors like non-compliance to use of ITNs/MBN; and non-usage of IPT during pregnancy, presence of mosquito breeding sites and bushes around the premises of the study participants were risk factors contributing to prevalence of malaria among pregnant women in Aboh Mbaise General Hospital in Imo State.
A Short Note on Dietary Components and Noncommunicable Diseases
Authors: N Rashed
Published: November 2020

Human nutrition and associated metabolism together with the development of non-communicable diseases are the key factors for the mass public health maintenance around the world. The dietary habits; i.e., the optimal consumption of the dietary factors, evaluation of the etiologic effects of the nutrients on the metabolic aftermaths, and assessment of the risk factors ofdifferent diets on the disease progression are hence major health legislative attributes. Current review shortly explained the impact of diets on public health sustainability.
Emotionality and Intertextuality in Official Discourses of Post-Soviet Authoritanian States (Kazakhstan, Belarus, and Russia)
Authors:   Vasyl Stus’ Donetsk National University, Steblyna Nataliia
Published: November 2020

Official discourses of Kazakhstan, Belarus and Russia are discovered with formal analysis in the article. The official discourses were represented with presidents’ web-sites. With the help of computer programs (Python language) the indicators of emotionality, direct and indirect intertextuality were calculated. The low emotionality and direct intertextuality are characteristic features of all three discourses. This underlines low intensity (or formality) of political interrelations. Average indicators of Kazakhstan and Russia discourses emotionality are (0,26; 0,24), for Belarus (0,39). These indicators are almost two times smaller, than in countries with more democratic regimes. Additionally, two types of authoritarian discourses are differentiated: more emotional and sporadic (Belarus) and less emotional stable (Kazakhstan, Russia). The positive correlation between the discourses of Kazakhstan and Russia (0,84) is discovered. Thus, similar periods of the discourses development are found: with more salient emotionality (before 2013) and unemotional period afterwards. For Belarus and Russia the periods with high indicators for indirect intertextuality (2012-2014, 2016-2017; and 2005-2010 – respectively) are discovered. The indicators’ values are explained with political interrelations imitation. The big amount of political texts (more than a half, sometimes – 80-90%) are congratulations and commemorations of authoritative leaders towards political actors’ form international community. The small period of such texts domination for Kazakhstan (2011-2012) is differentiated. Thus, the imitation of political interrelations with communication for Russia and Belarus is studied; however, this feature is not a characteristic one for Kazakhstan discourse. It’s concluded that all three discourses have artificial character of political interrelations in official discourses, which can be discovered with clear templates usage for all the political texts.
Disseminated acanthamoebiasis in a horse
Authors: Gonzalez Maria F. , Fresneda Karina , Carvallo Francisco
Published: November 2020

Amoebic infections have been described in humans and animals, causing lesions in specific organs or as systemic infections. In both animals and humans, immunocompromised patients are predisposed to the infection. The present report described the macroscopic and histologic findings in a Quarter horse filly who presented with a systemic infection caused by Acanthamoeba spp. that was characterized by multinodular lesions in lung, kidney, heart, submandibular lymph node, and lip. Histopathology revealed dense inflammatory infiltrates composed of neutrophils, histiocytes, occasional multinucleated giant cells, and numerous intralesional trophozoites. Immunohistochemistry was positive for Acanthamoeba spp trophozoites in lungs, kidneys, heart, submandibular lymph node, and lip. No predisposing conditions were identified.
Cutaneous myofibroblastic fibrosarcoma in a margay (Leopardus wiedii): a case report
Authors: Bermúdez-Salas Raúl , Campos Natalia , Barrantes Daniel , Arguedas Randall , Alfaro-Alarcón Alejandro
Published: November 2020

A routine check-up was performed on a captive 14-year-old female margay (Leopardus wiedii), a cutaneous mass was detected on the ventral thorax. The mass was surgically removed and sent for histopathological analysis. Histologically, the mass was a poorly-demarcated, highly cellular, infiltrative and unencapsulated mesenchymal neoplasm. Immunohistochemical labeling for smooth muscle actin and vimentin were positive, while desmin and cytokeratin were negative which is consistent with a myofibroblastic fibrosarcoma. This type of tumor has been diagnosed in wild felines, however this seems to be the first report of its occurrence in this L. wiedii. Wildlife oncology studies should be performed to promote our understanding of cancer in a species.
Health Leadership Lessons From a Fine Detective
Authors: Arya Dinesh
Published: November 2020

Objective: To explore lessons on leadership from interactions and success of two very fine detectives – Sherlock Holmes and Dr. Watson Methods: From the review of Sir Arthur Conan Doyle's depiction of Sherlock Holmes, some clear and useful lessons have been drawn for health leaders. An erratic, unsystematic Sherlock Holmes and an analytical, systematic and introspective Dr. Watson provide useful lessons for health leaders on trust, teamwork and how complementary abilities enable the achievement of a great outcome. Conclusions: It is really important for health leaders to not forget the elementary message to remain focused on care provision, enable the provision of care that the community wishes to receive and avoid of the temptation of making decisions without appropriate information set to inform those decisions.
Terapia ocupacional para atendimento odontológico de pacientes especiais
Authors: Spezzia Sérgio
Published: November 2020

A terapia ocupacional pode ser aplicada no setor odontológico, modificando o ambiente dos consultórios e ambulatórios no que tange à decoração, além de adotar a música no ambiente com o objetivo de minimizar a tensão dos indivíduos no transcorrer dos procedimentos. Com o recurso da terapia ocupacional pode-se ainda fazer emprego do mobiliário, objetivando melhorar a postura dos pacientes nas cadeiras odontológicas. Nesse contexto, torna-se extremamente importante tecer orientações e embasamento à respeito das medidas adotadas e de seus benefícios, orientando tanto o cirurgião dentista como a família dos pacientes. No âmbito odontológico, pacientes especiais podem ser inseridos em nove grupos, são eles: doenças sistêmicas crônicas; doenças infectocontagiosas; condições sistêmicas; anomalias congênitas; deficiência física; deficiência mental; transtornos psiquiátricos; distúrbios comportamentais e distúrbios sensoriais e de comunicação. O terapeuta ocupacional faz uso dos conhecimentos médicos e sociais para dar andamento aos recursos terapêuticos e para atuar na assistênciaodontológica aos pacientes especiais. Busca-se um melhor posicionamento desses pacientes na cadeira do equipo odontológico que se acha instalado para utilização nos atendimentos no decorrer dos procedimentos dentários a serem executados; inibição de movimentos involuntários e a humanização do ambiente, procurando adaptações que sejam condizentes com as necessidades presentes em particular. O objetivo do presente artigo foi verificar a possível inter-relação existente entre terapia ocupacional e a assistência à saúde prestada em odontologia voltada aos pacientes especiais. Realizou-se revisão narrativa de literatura com busca nas bases de dados: Google Acadêmico, PubMED envolvendo levantamento de estudos e artigos que versavam sobre a utilização da terapia ocupacional no atendimento odontológico aos pacientes especiais. No Google Acadêmico buscou-se dar ênfase às pesquisas realizadas entre 2014 e 2019. No PubMED considerou-se todo o acervo disponível na base de dados quando da realização da pesquisa, independentemente da data de publicação dos trabalhos. A utilização da terapia ocupacional a nível odontológico age favoravelmente nos indivíduos, podendo melhorar a qualidade de vida dos pacientes especiais em tratamento.
Optimizarea modalitãților de semnalizare a erorilor în interacțiunea om-calculator
Authors: Maricuțoiu Laurențiu P
Published: November 2020

In Human-Computer Interaction, error signaling has negative effects both on user’s cognitive efficiency, andon user’s emotional state. Previous research indicated that such effects are the result of surface processing of errormessages. This study aims at analysing the relations between the psysical characteristics of error messages and userreactions (cognitive and emotional). Study participants were 110 students (48% male), distributed in 8 experimentalconditions. Results indicated that the pictogram specific to the error message and the presence of the word „error“in the body of the message lead to the apparition of the cognitive and emotional effects we previously described
Examinarea dimensiunilor comportamentului de tip A în relația dintre stresorii ocupaționali și reacțiile la stres în munca de poliție
Authors: Rus Claudia Lenuța , Lazăr Anca-Maria
Published: November 2020

The literature has highlighted the presence of a higher proportion of Type A behavior among police officerscompared to the general population. Thus, the present study examined the predictive role of two components ofType A behavior (impatience and drive) on stress reactions and the moderator effect of these dimensions in therelation stressors-reactions to stress in police work. To assess the variables included in this study it was usedscales from Occupational Stress Indicator-2 (OSI-2; Cooper, Sloan, & Williams, 1988). These scales were completedby 108 policemen. The results showed that impatience is the only predictor of some aspects of physical(tempered behavior, energy) and mental health (satisfaction, peace of mind). The data show that impatience anddrive do not moderate the relationship between stressors and reactions to stress represented by work satisfaction,satisfaction toward organization, satisfaction with mental well-being, peace of mind, tempered behavior and energy.The impatience has moderated the tension generated by the workload and responsibilities on activism. Thedrive has moderated the relation between workload and activism. Based on these results, it has been discussedthe validity of the conceptual model of the OSI-2 and the development of occupational stress management interventionsin police work.
„Infidelitãțile“ coeficientului de fidelitate Cronbach alfa
Authors: Popa Marian
Published: November 2020

Cronbach alpha coefficient is still commonly used in research dedicated to the development of psychologicaltests. However, there is a certain lack of understanding of the significance of real and, especially, has its limits.The article presents the fundamental postulates of classical test theory and analyzes, in this context, the mainissues affecting the calculation and interpretation of Cronbach alpha coefficient: unidimensionality, internal consistency,item number, characteristics of the data, the sampling error. Finally, are summarized recommendationsof good practices on the use and reporting Cronbach's alpha.
Maş Fasulyesi [Vigna radiata (L.) Wilczek] Genotiplerinin Isparta Koşullarında Fenolojik Özellikler Yönünden Karakterizasyonu
Authors: Karaman Ruziye , Kaya Muharrem
Published: November 2020

Çalışma, ülkemizdeki farklı illerden toplanan maş fasulyesi tohum materyallerinin Isparta koşullarında fenolojik özelliklerinin belirlemesi amacıyla Isparta Uygulamalı Bilimler Üniversitesi (ISUBÜ) Ziraat Fakültesi Tarla Bitkileri Bölümü deneme tarlalarında, yürütülmüştür. Çalışmada 2015 yılında 91 adet materyal toplanmış ve aynı yıl tohum çoğaltılması ve ön gözlemler yapılmıştır. Ön gözlem sonuçlarına göre, seçilen 50 adet maş fasulyesi genotipi, 4 tescilli çeşit ile birlikte 2017 ve 2018 yıllarında tarla denemelerine alınmıştır. Araştırma, Augmented Deneme Desenine göre 5 tekerrürlü olarak yürütülmüştür. Denemede fenolojik özellikler olarak çıkış süresi, çiçeklenme süresi, bakla bağlama süresi ve vejetasyon süresi incelenmiştir. Çalışmada sonuç olarak, tescilli çeşitlerden daha erkenci özelliğe sahip genotiplerin olduğu ortaya çıkmıştır. Genotiplerin içerisinde en erkenci olanının 27 S 08 genotipi olduğu tespit edilmiş olup, bu genotipin ileride ümitvar olduğu düşünülmektedir.
Et ve Süt Kurumunun Sözleşmeli Kırmızı Et Satış Politikasının Tüketiciler Boyutunda Değerlendirilmesi
Authors: Çiçek Adnan , Ayyıldız Merve , Doğar Didem
Published: November 2020

Türkiye‘de kırmızı et fiyatlarında istikrar sağlamak amacıyla Et ve Süt Kurumu (ESK), zincir marketler aracılığı ile kırmızı et satışı uygulanmaktadır. Bu politikanın tüketiciler perspektifinden etkinliğini değerlendirmek amacıyla çalışmada, Gaziantep il merkezindeki 388 hane halkından elde edilen veri seti kullanılmıştır. Elde edilen bulgulara göre, tüketicilerin %53,6‘sının kırmızı et satın alma yerini belirlerken fiyatlardaki uygunluk sebebi ile yüksek oranda anlaşmalı marketleri tercih ettiği belirlenmiştir. Tüketicilerin tamamı anlaşmalı marketlerden en az bir kez kırmızı et alırken %62,11‘nin sonrasında bu marketlerden kırmızı et satın almaktan vazgeçtikleri belirlenmiştir. Bu durum oldukça dikkat çekici bulunmuş ve hanelerin anlaşmalı marketlerden kırmızı et satın alımına devam etme durumunu etkileyen faktörleri belirlemek için logit modelden yararlanılmıştır. Logit model sonuçlarına göre, hanelerin anlaşmalı marketlerden kırmızı et satın almasını kırmızı et fiyatını önemseme, gelir ve bu marketlerden alışveriş yapma sıklığı değişkenlerinin etkilediği tespit edilmiştir. ESK‘nun anlaşmalı marketler aracılığıyla sözleşmeli kırmızı et satışına yönelik uygulanan politikanın fiyat istikrarını sağlayıcı nitelikte olmasından ziyade, gelir düzeyi düşük kesime kırmızı et satışı yapmaya yönelik olduğu, talep arttırıcı bir etkide bulunmadığı ifade edilebilir.
Türkiye‘de Organik Tarım
Authors: Tıraşcı Serpil , Erdoğan Ümmügülsüm , Aksakal Vecihi
Published: November 2020

Dünyada olduğu gibi Türkiye‘de de gelişen bilim, teknoloji ve sanayi ile buna bağlı olarak değişen kırsal kalkınma ve üretim anlayışı, tarımsal faaliyetlerin gerçekleşme şeklini değiştirmiştir. Yeni üretim metotları sonucu çevre ve insan sağlığı bozulmuş ve gelinen noktada bilim insanları organik tarıma yönelmiştir. Ülkemizde 1980‘li yıllarda başlayan organik tarım uygulamaları, 1994 yılında organik tarım yönetmeliğinin yayınlanmasıyla önemli ölçüde gelişme göstermiş ve yönetmelik son olarak 2018 yılında düzenlenmiştir. Ülkemiz toprak ve su kaynakları, iklimi, ürün çeşitliliği bakımından organik tarıma elverişli koşullara sahip olması dolayısıyla organik tarımın yıllar içerisinde nasıl bir ilerleme gösterdiğini ve bugün ne aşamada olduğunu görebilmek amacıyla bu çalışma hazırlanmıştır. Türkiye‘ de 1990 yılında 1.037 ha alanda 313 çiftçi ile 8 organik ürün üretilmiştir. Son verilere göre 73.563 çiftçi 626.885 ha organik tarım alanında 2.371.612 ton organik ürün yetiştirmiştir. Ayrıca üretimi yapılan organik ürün çeşidi de 213‘e çıkarılmıştır. İhracat durumuna bakıldığında toplam ihracat miktarının 111.690.675 ton olduğu ve bu ihracat miktarının önemli bir miktarını 41.633.896 tonla buğday ve buğday ürünlerinin oluşturduğu görülmektedir. İthalat verilerine bakıldığında ise toplam ithalat miktarının 175.865,85 ton olduğu ve en çok ithal edilen ürünün ise soya fasulyesi (tohumluk olmayan) (99.446 ton) olduğu bilinmektedir. Geçmiş yıllara göre organik tarım üretiminde önemli bir artış gözlense de Türkiye‘nin tarım potansiyeli göz önünde bulundurulduğunda bu artışın daha da artabileceği öngörülmektedir.
The use of information technology by restaurants in Joglosemar, the mainstay city of Indonesian culinary
Authors: Asrihapsari Andi , Setiawan Doddy
Published: November 2020

According to the Indonesian Ministry of Tourism, Yogyakarta, Surakarta, Semarang (Joglosemar) are top culinary destinations in Indonesia, with information technology (IT) needed to strengthen its development. Therefore, this study examines IT's adaptation in restaurants owned by micro, small, and medium enterprises (MSMEs) at Joglosemar. This is a descriptive research with data obtained from 200 restaurants each in three cities through questionnaires, interviews, and observation, culminating in 600 restaurants. The result showed that restaurants adopt IT contact (92%), online transportation partner (81%), non-cash cashier payment (66%), IT cashier (61%), Wi-Fi (57%), and large orders-outside restaurant (49%) to improve their performance. This research contributes to providing a current overview of the development of IT restaurants in Indonesia, therefore it is expected to enable the entrepreneur to be more ready to face the business competition in the digital era. This research is limited to Joglosemar, therefore further study needs to investigate other top culinary cities. Secondly, this is a descriptive research on the adoption of IT by restaurants in Joglosemar, therefore further study needs to elaborate on the determinants factors of adoption of IT by restaurants and its effects on performance.
Biographical and artistic in M. Y. Lermontov’s novel “Princess Ligovskaya” and the drama “Two Brothers”
Authors: Denisova Ekaterina Andreevna
Published: November 2020

This article is dedicated to a comparative analysis of two works by M. Y. Lermontov, which represent the interpretation of the common biographical context. The subject of this research is the specificity of transformation of the biographical fact into the artistic generalization in the works of M. Y. Lermontov. The object of this research is the drama "Two Brothers" and the novel Princess Ligovskaya” viewed within the paradigm of artistic comprehension of the scenario of unfaithfulness, or destruction of love as conceptually important in the conflict present in the works of M. Y. Lermontov. Such approach, based on interrelation between the creative and biographical, allows making important remarks on specificity of the phenomenon of autobiographism in the works of M. Y. Lermontov. The author analyzes the initial biographical situation (relationship between Lermontov and V. A. Lopukhina), and concludes that two literary texts – drama and novel – represent a distinct system of creative reflection of the biographical fact. It is established that in the process of creating the drama, and the novel later on, Lermontov ensues the need for artistic interpretation and resolution of the situation of unfaithfulness as an instance, and in a broader sense – as an ontological pattern; however, the conflict cannot be settled within the framework of this storyline. Since 1837, autobiographism in Lermontov's works ceases to play the crucial role; in other words, personal situation and its artistic interpretation no longer coincide, which renders impossible to continue the novel. Such circumstance allows realizing the reasons of incomplete works of Lermontov’s early period, which lie in inability to resolve love conflicts with a positive finale.
Lexical peculiarities of French language of Switzerland in the context of linguistic worldview (based on the materials of the thematic group “Horticulture”)
Authors: Afonina Maria Mikhailovna
Published: November 2020

The goal of this research is the analysis of linguistic aspect of the fragment of linguistic worldview of the francophone part of Switzerland. Due to the fact that linguistic worldview is a mental-lingual formation, it incorporates linguistic and extralinguistic side of reality, as well as reflects correlation between them. Analysis is conducted on the specific lexical units of the Swiss French language; they are compared to analogues in the language of metropolitan country for the purpose of revealing the features that convey extralinguistic information. The subject of this research is the ethnolinguistic characteristics of helvetisms – linguistic peculiarities that reflect national-cultural specificity of Swiss ethnic group and are absent in the French language of France. The scientific novelty consists in consideration of the fragment of linguistic worldview of French-speaking Switzerland related to horticulture, using a set of methods for the analysis of various characteristics of the word or phrase as an independent linguistic unit. In the course of study, the author applies semantic analysis of the word, analysis of etymology of the word, analysis of inner form of the word. The acquired results contain information on ethnocultural content of such characteristics helvetisms, as external form of the word, inner form of the word, origin and meaning of the word. It was established in majority of cases, ethnocultural information is usually conveyed by such characteristics of lexical unit as external form of the word or phrase, or separate components of the structure of word meaning. The presented results can find practical implementation in cross-cultural communication, lexicographic, and cognitive linguistics.
Monolog Drama ”Matinya Toekang Kritik” Karya Agus Noor (Kajian Teori Reaksi)
Authors: Suprihatien Suprihatien , Damayanti Rini
Published: November 2020

Tujuan menganalisis monolog drama ini  untuk mengetahui sampai sejauh mana suatu ironi dan parodi yang ada pada Monolog Drama ”Matinya Toekang Kritik”  Karya Agus Noor dalam Kajian Teori Reaksi.  Metode  deskriptif kualitatif digunakan untuk pembahasan monolog drama ini dengan objek penelitian berupa naskah drama karya Agus Noor yang berjudul “Matinya Toekang Kritik”. Metode kepustakaan  digunakan dalam penelitian ini  karena yang diteliti berupa naskah tertulis.  Teknik yang digunakan pada  penelitian ini berupa  teknik baca dan teknik catat. Dasar kajian berupa  teori reaksi dalam  naskah monolog drama ini. Monolog drama atau lakon ini berani menyampaikan kritik  berbagai fenomena kebudayaan, dari mulai masalah ekonomi, sejarah, politik, sampai masalah sosial budaya. Dari kritikan para kritikus ini dapat tersampaikan   suatu sindiran   mulai  yang nakal sampai bernada konyol. Berbagai ironi dan parodi dapat  dijumpai pada naskah monolog drama ini.
Pengaruh Discovery Learning Terhadap Kemampuan Menulis Puisi Siswa Kelas VIII MTs Negeri 2 Sukabumi
Authors: Robiyadin Robiyadin , Supendi Deden Ahmad , Firdaus Asep
Published: November 2020

Tujuan penelitian ini untuk mendeskripsikan langkah-langkah metode pembelajaran discovery learning dalam pembelajaran menulis puisi serta hasil menulis sebelum dan setelah implementasi. Lokasi penelitian dilakukan di MTs Negeri 1 Sukabumi tahun ajaran 2019/2020. Penelitian ini menggunakan metode eksperimen. Prosedur penelitiannya dilakukan dengan tiga tahapan yaitu pelaksanaan tes awal menulis puisi, implementasi discovery learning dan tes akhir menulis puisi. Purposive sampling digunakan sebagai teknik pemngambilan sampel dengan berdasarkan karakteristik dan rekomendasi guru pengampu mata pelajaran bahasa Indonesia. Sampel yang dipilih yaitu siswa kelas VIII 5. Hasilnya penelitian ini menunjukan bahwa implementasi discovery learning dalam pembelajaran dapat dilaksanakan dengan tahapan, yaitu: pendahuluan pembelajaran dengan penyampaian tujuan pembelajaran menulis puisi; pembentukan kelompok belajar; pemberian stimulus teks puisi yang difasilitasi oleh guru; mengdeintifikasi permasalahan mengenai proses kreatif menulis puisi; mengumpulkan data dari berbagai sumber mengenai proses kreatif menulis puisi; mengolah data yang diperoleh dengan bimbingan guru melalui penciptaan teks puis; menyampaikan hasil diskusinya, dan menyimpulkan proses penulisan kreatif menulis puisi. Adapun hasil menulisnya terdapat kenaikan yang signifikan dari rata-rata tes awal sebesar 76 menjadi 82.
Teknik Persuasif pada Paidpromote Produk di Instagram Fanbase K-Pop Indonesia
Authors: Kulup Luluk Isani , Nursafitri Anisa
Published: November 2020

Penelitian ini dibuat untuk menjelaskan teknik persuasif yang digunakan dalam promosi produk berbayar di instagram fanbase K-POP Indonesia. Metode penelitian yang digunakan adalah kualitatif. Data dalam penelitian ini adalah semua tulisan pada caption yang tertera pada unggahan produk yang diiklankan di Instagram. Sumber data penelitian ini diambil dari iklan produk yang diunggah ke akun fanbase KPOP Indonesia di Instagram. Teknik pengumpulan data menggunakan teknik dokumentasi, dilakukan screenshot iklan di akun fanbase KPOP Indonesia. Hasil studi Promosikan Produk Berbayar di Instagram Fanbase K-POP Indonesia didapatkan semua teknik yang dijelaskan oleh Keraf (2007). Teknik persuasif tersebut adalah teknik sugesti, teknik substitusi, teknik konformitas, teknik rasionalisasi, teknik identifikasi, teknik kompensasi, dan teknik proyeksi.
Demographic determinants of regional development in Eastern Poland
Authors: Bugowski Łukasz Karol
Published: November 2020

Research Background: Demography affects human capital, which today is one of the most important factors of regional development. Demographic factors create possibilities or limit the expansion of the knowledge and skills and condition socio-economic activity. In other words, demography can favour or block regional development. Negative demographic trends, in particular referring to the size and structure of the population as well as population growth, are a significant challenge of regional policy. In addition, in the case of regions with a lower level of development, the migration rate is negative, which in turn may limit regional development. As a consequence, the following hypothesis will be verified in the article: demographic processes are a barrier to the development of regions in Eastern Poland.Purpose of the paper: The main objective of the article is to analyze the spatial diversity of demographic phenomena in Eastern Poland, along with the identification of areas with unfavorable demographic developmental conditions. Implementation of the second objective is to carry out the analysis at the level of subregions, not regions, which will allow for more precise analysis of negative demographic phenomena.Methodology/Methods/Data sources: In the article the method of linear ordering of objects using the taxonomic measure of Z. Hellwig's development is used. In addition, a comparative analysis of the main demographic phenomena in 2010, 2013 and 2016 in Eastern Poland was carried out. The main source of data used in the article was the Statistics Poland (Local Data Bank). Findings: The analysis allows to conclude that in 2010-2016 in Eastern Poland an increase in the demographic burden resulting from the aging population was observed. In addition, the migration rate is negative and sub-replacement fertility rate is common in the majority of the subregions. As a consequence, negative demographic phenomena are increasingly limiting human capital and indirectly have a negative impact on regional development. However, the conducted analysis allows to distinguish subregions with positive values of the analyzed indicators, but it refers only to a few regions including a capital of a given voivodeships, and do not significantly affect the negative general demographic trends in Eastern Poland.
Molecular characterization of gene encoding halo tolerant amylase of Bacillus paralicheniformis isolated from Jazan region
Authors: Ghazouani Abdullah , El-Gayar Khaled , Abada Emad
Published: November 2020

Objectives:

This study aims to characterize the gene encoding halo tolerant amylase of bacteria isolated from Jazan region.

Materials and Methods:

Soil samples were collected from several area of Jazan region, KSA. The samples were serially diluted and plateted on starch agar plates. The amylase producing bacteria were detected by iodine test. To determine the halophilic amylase producing bacteria, several colonies were tested for their ability to grow at higher concentrations of NaCl ranging from 7 to 16%. The bacteria was identified by 16S rRNA and the full length amylase gene was fully identified by sequencing using specific primers.

Results:

One bacterial halophilic isolate was able to grow on starch agar medium up to 14% NaCl. The Gram stain of the isolate indicated that it is Gram-positive, bacilli. The 16S rRNA gene homology study showed that the bacterial isolate was identified as Bacillus paralicheniformis. Two specific primers were designed named S1F, S1R, to amplify the amylase gene (AMY) region using PCR and the PCR product was sequenced. The sequencing results showed that the full-length amy gene of B. paralicheniformis was of 1452 encoding 483 amino acids. The expected M.Wt. of the protein expressed is of 55 KDa.

Conclusion:

We report the isolation, identification, and characterization of an isolate of halophilic bacterium isolated from Jazan region. Based on molecular identification, this isolate was identified as Bacillus paralicheniformis. This bacterial strain has an α-amylase gene in its genome and is able to produce extracellular α-amylase. Based on the findings of this work we propose that Bacillus paralicheniformis amy gene could be cloned into expression vector for large scale production.
Leber's hereditary optic neuropathy plus, case report
Authors: Короткова Д.Г. , Карпова М.И. , Буянова Г.В. , Кашко Т.Н.
Published: November 2020

Наследственная оптическая невропатия Лебера (LHON) - митохондриальное заболевание с атрофией зрительного нерва. Хотя в большинстве случаев LHON других ассоциированных неврологических отклонений нет, сообщалось о случаях LHON plus. В статье представлен анализ клинического случая с проявлением неврологических симптомов в подростковом возрасте.

Leber’s hereditary optic neuropathy (LHON) is a mitochondrial disorder with optic nerve atrophy. Although there are no other associated neurological abnormalities in most cases of LHON, cases of “LHON plus” have been reported. The article presents an analysis of clinical case with the manifestation of neurological symptoms in adolescence.
Sign language prevalence as a source of data for genetic epidemiology of inherited forms of hearing loss
Authors: Романов Г.П. , Пшенникова В.Г. , Лашин С.А. , Соловьев А.В. , Терютин Ф.М. , Сазонов Н.Н. , Хуснутдинова Э.К. , Посух О.Л. , Федорова С.А. , Барашков Н.А.
Published: November 2020

На основе материалов тотальной переписи населения (2010 г.) проведен анализ распространенности языка жестов в России. Полученные результаты сопоставлены с имеющимися данными о частоте наследуемых форм потери слуха на территории России.

The paper presents the results of an analysis of the prevalence of sign language (SL) in Russia collected during the 2010 total census, as well as their comparison with data on the frequency of hereditary forms of hearing loss.
The frequency of heterozygous carriage of CFTR gene mutations causing the development of cystic fibrosis in a population-based cohort study (ESSE-Vologda)
Authors: Сотникова Е.А. , Климушина М.В. , Киселева А.В. , Скирко О.П. , Курилова О.В. , Дивашук М.Г. , Хлебус Э.Ю. , Козлова В.А. , Покровская М.С. , Сломинский П.А. , Мешков А.Н. , Драпкина О.М.
Published: November 2020

Целью работы было создание панели для скрининга носительства мутаций, связанных с развитием муковисцидоза (МВ), и апробация панели в популяционной выборке ЭССЕ-Вологда. В исследование вошли 642 участника из популяционной выборки региона Вологды. Детекцию мутаций осуществляли с помощью системы QuantStudio 12K Flex Real-Time PCR. Создана скрининговая панель, включающая 60 мутаций гена CFTR. Среди 642 участников исследования выявлено 23 гетерозиготных носителя 6 мутаций, связанных с развитием МВ, частота носительства составила 3,58% (ДИ 95%: 2,28-5,33%) или 1:28 чеовек. Разработанная панель может быть использована для скрининга гетерозиготных носителей МВ.

The aim of the study was to create a panel for screening the carriers of mutations associated with the development of cystic fibrosis (CF), and testing the panel on 642 participants of the population-based cohort study (ESSE-Vologda). Variants were detected using the QuantStudio 12K Flex Real-Time PCR system. A custom panel that includes 60 CFTR variants was created. Among 642 participants in the study, 23 heterozygous carriers of 6 CFTR mutations were identified. The carrier frequency was 3.58% (CI95%: 2.28-5.33%) or 1:28. The custom panel can be used for screening heterozygous CF carriers.
Development and application targeted NGS panels in the selective screening algorithm for inborn errors of metabolism. An experience of the St. Petersburg Medical and Genetic Center
Authors: Чурюмова Ю.А. , Вохмянина Н.В. , Шляга С.В. , Вавилова Т.В. , Симакова Т.С. , Зайцева М.А. , Павлов А.Е.
Published: November 2020

Наследственные болезни обмена веществ представляют собой обширный класс генетических заболеваний и вносят значительный вклад в детскую заболеваемость, при этом их диагностика с использованием биохимических методов зачастую вызывает затруднения. В СПбГКУЗ МГЦ были разработаны и внедрены три панели для секвенирования 88 генов, ответственных за развитие трех групп наследственных болезней обмена (НБО), и протестировано 84 ребенка, у которых данные заболевания были заподозрены по данным тандемной масс-спектрометрии (ТМС), либо по наличию клинических симптомов. У 6 детей методом NGS полностью установлена генетическая причина заболевания. Патогенные мутации выявлялись значительно чаще при повышении биохимических маркеров, демонстрируя ведущую роль предварительного биохимического скрининга в проведении NGS анализа. NGS значительно повышает результативность клинической диагностики НБО. Биохимическое тестирование и NGS играют взаимодополняющие роли, и их комплексное использование в алгоритме селективного скрининга позволяет повысить точность диагностики НБО.

Inborn errors of metabolism are an extensive class of genetic diseases and contribute significantly to childhood morbidity, and their diagnosis using biochemical methods is often difficult. Three panels for sequencing of 88 genes responsible for the development of three groups of inborn errors of metabolism (IEM) were developed and introduced in St.Petersburg Medical and Genetic Center and 84 children were tested for which these diseases were suspected by tandem mass-spectrometry or by the presence of clinical symptoms. In 6 children, the NGS method fully established the genetic cause of the disease. Pathogenic mutations were detected significantly more frequently with increased biochemical markers, demonstrating the leading role of pre-biochemical screening in performing NGS analysis. NGS significantly improves the clinical diagnostic effectiveness of IEM. Biochemical testing and NGS play complementary roles and their complex use in selective screening algorithm allows to increase accuracy of IEM diagnostics.
ANALISIS KUALITAS AIR DAERAH IRIGASI LUBUK ANTUK KECAMATAN HULU GURUNG KABUPATEN KAPUAS HULU
Authors: Wahyu Zainal , Maulina Syarifah Melly , Widodo Muji Listyo
Published: November 2020

Desa Lubuk Antuk Kecamatan Hulu Gurung memiliki lahan daerah irigasi fungsional seluas 38,11 Ha. Saat ini petani maupun pemerintah daerah Kabupaten Kapuas Hulu belum mengetahui apakah air daerah irigasi Lubuk Antuk masih sesuai atau tidak untuk irigasi. Untuk menjawab kekhawatiran terhadap kualitas air irigasi yang digunakan maka perlu dilakukan penelitian kualitas air irigasi di Daerah Irigasi Lubuk Antuk. Pengambilan data untuk penelitian ini terdiri dari dua bagian, yaitu pengambilan sampel air di lapangan dan uji kualitas air di laboratorium. Dari hasil pemeriksaan kualitas air permukaan, dapat dilihat bahwa hampir semua parameter pencemaran air di lokasi masih berada dibawah baku mutu lingkungan kategori Air Kelas III berdasarkan Peraturan Pemerintah No. 82 Tahun 2001 tentang Pengelolaan Kualitas Air dan Pengendalian Pencemaran Air. Hanya satu parameter di titik lokasi yang melebihi baku mutu, yaitu BOD. Sehingga dapat dikatakan bahwa kualitas air irigasi di Daerah Irigasi Lubuk Antuk masih memenuhi baku mutu yang telah ditetapkan
Measuring the Concentration of Protein Nanoparticles Synthesized by Desolvation Method: Comparison of Bradford Assay, BCA Assay, hydrolysis/UV Spectroscopy and Gravimetric Analysis
Authors: Khramtsov Pavel , Kalashnikova Tatyana , Bochkova Maria , Kropaneva Maria , Timganova Valeria , Zamorina Svetlana A. , Rayev Mikhail B.
Published: November 2020

Research paper on sunthesis of protein nanoparticles

AbstractThe desolvation technique is one of the most popular methods for preparing protein nanoparticles for medicine, biotechnology, and food applications. We fabricated 11 batches of BSA nanoparticles and 2 batches of gelatin nanoparticles by desolvation method. BSA nanoparticles from 2 batches were cross-linked by heating at +70 °C for 2 h; other nanoparticles were stabilized by glutaraldehyde. We compared several analytical approaches to measuring their concentration: gravimetric analysis, bicinchoninic acid assay, Bradford assay, and alkaline hydrolysis combined with UV spectroscopy. We revealed that the cross-linking degree and method of cross-linking affect both Bradford and BCA assay. Direct measurement of protein concentration in the suspension of purified nanoparticles by dye-binding assays can lead to significant (up to 50-60%) underestimation of nanoparticle concentration. Quantification of non-desolvated protein (indirect method) is affected by the presence of small nanoparticles in supernatants and can be inaccurate when the yield of desolvation is low. The reaction of cross-linker with protein changes UV absorbance of the latter. Therefore pure protein solution is an inappropriate calibrator when applying UV spectroscopy for the determination of nanoparticle concentration. Our recommendation is to determine the concentration of protein nanoparticles by at least two different methods, including gravimetric analysis.
Frustrated Flexibility in Metal-Organic Frameworks
Authors: Pallach Roman , Keupp Julian , Terlinden Kai , Frentzel-Beyme Louis , Kloß Marvin , Machalica Andrea , Kotschy Julia , Vasa Suresh K. , Chater Philip A. , Sternemann Christian , Wharmby Michael T. , Linser Rasmus , Schmid Rochus , Henke Sebastian
Published: November 2020

Stimuli-responsive flexible metal-organic frameworks (MOFs) remain at the forefront of porous materials research due to their enormous potential for various technological applications. Here, we introduce the concept of frustrated flexibility in MOFs, which arises from an incompatibility of intra-framework dispersion forces with the geometrical constraints of the inorganic building units. Controlled by appropriate linker functionalization with dispersion energy donating alkoxy groups, this approach results in a series of MOFs exhibiting a new type of guest- and temperature-responsive structural flexibility characterized by reversible loss and recovery of crystalline order under full retention of framework connectivity and topology. The stimuli-dependent phase change of the frustrated MOFs involves non-correlated deformations of their inorganic building unit, as probed by a combination of global and local structure techniques together with computer simulations. Frustrated flexibility may be a common phenomenon in MOF structures, which are commonly regarded as rigid, and thus may be of crucial importance for the performance of these materials in various applications.
Data augmentation strategies to improve reaction yield predictions and estimate uncertainty
Authors: Schwaller Philippe , Vaucher Alain C. , Laino Teodoro , Reymond Jean-Louis
Published: November 2020

Chemical reactions describe how precursor molecules react together and transform into products. The reaction yield describes the percentage of the precursors successfully transformed into products relative to the theoretical maximum. The prediction of reaction yields can help chemists navigate reaction space and accelerate the design of more effective routes. Here, we investigate the best-studied high-throughput experiment data set and show how data augmentation on chemical reactions can improve yield predictions' accuracy, even when only small data sets are available. Previous work used molecular fingerprints, physics-based or categorical descriptors of the precursors. In this manuscript, we fine-tune natural language processing-inspired reaction transformer models on different augmented data sets to predict yields solely using a text-based representation of chemical reactions. When the random training sets contain 2.5% or more of the data, our models outperform previous models, including those using physics-based descriptors as inputs. Moreover, we demonstrate the use of test-time augmentation to generate uncertainty estimates, which correlate with the prediction errors.
Etyka w „Niezbędniku badacza”. Kwestie etyczne w podręcznikach metod badań jakościowych
Authors: Ślęzak Izabela
Published: November 2020

Podręczniki omawiające metody badań stanowią ważny element socjalizacji początkujących badaczy, wprowadzając ich także w zagadnienia etyki badań społecznych. Celem artykułu jest analiza tego, jak przedstawiane są zagadnienia etyczne w ośmiu podręcznikach serii „Niezbędnik Badacza” Wydawnictwa Naukowego PWN. W wyniku jakościowej analizy treści zrekonstruowano główne wątki etycznej refleksji autorów. Koncentrują się oni na proceduralnych wymogach dotyczących etyki oraz głównych zasadach etycznych obowiązujących w badaniach jakościowych (świadoma zgoda, poufność), w niewielkim stopniu omawiając bardziej zniuansowane kwestie etyczne oraz współczesne wyzwania, z jakimi mierzą się badacze.
Pisemna zgoda? Argumenty badaczy jakościowych za i przeciw pisemnej zgodzie
Authors: Surmiak Adrianna
Published: November 2020

Uzyskanie świadomej i dobrowolnej zgody uczestnika badań należy do podstawowych zasad etycznych w badaniach społecznych. W krajach anglosaskich, gdzie kultura audytu jest bardzo dobrze rozwinięta, często wymaga się od badaczy jakościowych, by wzięli od badanych pisemną zgodę na udział w badaniach. WPolsce wraz z wprowadzaniem kultury audytu do szkolnictwa wyższego i nauki także mogą pojawić się takie oczekiwania wobec badaczy, na przykład ze strony grantodawców. Niewiele jednak wiadomo o stosunku rodzimych badaczy jakościowych do tej praktyki badawczej ani o ich doświadczeniach związanych z jej zastosowaniem. Artykuł omawia argumenty badaczy za i przeciw używaniu pisemnej zgody w społecznych badaniach jakościowych. Podstawą rozważań są opinie i doświadczenia socjologów i antropologów społeczno-kulturowych dotyczące wykorzystania pisemnej zgody w badaniach jakościowych głównie z udziałem osób podatnych na zranienie i marginalizowanych.
Analiza praktyk anonimizacji danych w polskich jakościowych badaniach socjologicznych na tle literatury przedmiotu oraz zasad etyczno-prawnych
Authors: Męcfal Sylwia
Published: November 2020

Celem artykułu jest przedstawienie praktyk anonimizacji danych stosowanych w jakościowych badaniach społecznych polskich socjologów oraz stworzenie typologii stosowanych rozwiązań. Aby sprawdzić, jak badacze prezentują sposoby anonimizacji danych, przeanalizowałam artykuły opublikowane w latach 2015–2019 w czterech ważnych polskich czasopismach socjologicznych. Na podstawie analizy wyróżniłam różne sposoby anonimizacji danych stosowane przez autorów artykułów, które poddane dodatkowej analizie skupień utworzyły cztery strategie anonimizowania danych: anonimizacji domyślnej podstawowej, z wiodącą rolą pseudonimizacji, anonimizacji rozszerzonej o anonimizację miejsca oraz anonimizacji rozszerzonej o charakterystykę cech na poziomie zbiorowości, a nie jednostki. Powstałe typy anonimizacji stanowią odzwierciedlenie typów występujących w literaturze przedmiotu i stanowią potwierdzenie tego, że badacze w znacznej części stosują anonimizację jako procedurę naturalną i nieodłączną część badań (zgodnie z zapisami „Kodeksu etyki socjologa” PTS). Używanie prawdziwych tożsamości uczestników badania oraz autoryzacja stanowią rzadkość. Wybierane strategie anonimizacji mają często postać prostej anonimizacji domyślnej, rzadziej stosowane są rozszerzone typy anonimizacji.