bluuebunny/crossref_metadata_2025 huggingface dataset:
Hard x-ray – optical four-wave mixing using a split-and-delay line
Authors: Peters William K. , Feltman Jacob , Jones Travis , Song Sanghoon , Chollet Matthieu , Robinson Joseph , Padmanabhan Prashant , Foglia Laura , Bencivenga Filippo , Coffee Ryan , Bowlan Pamela
Published: August 2023

New, hard x-ray free electron lasers (FEL) produce intense femtosecond-to-attosecond pulses at angstrom wavelengths, giving access to the fundamental spatial and temporal scales of matter. These revolutionary light sources open the door to applying the suite of nonlinear, optical spectroscopy methods at hard x-ray photon energies. Nonlinear spectroscopy with hard x-rays can allow for measuring the coherence properties of short wavelength excitations with atomic specificity and for understanding how high energy excitations couple to other degrees of freedom in atomic, molecular or condensed-phase systems. As a step in this direction, here we present hard x-ray, optical four-wave mixing (4WM) measurements done at 9.8 keV at the split-and-delay line at the x-ray correlation spectroscopy (XCS) hutch of the Linac Coherent Light Source (LCLS). In this work, we create an x-ray transient grating (TG) from a pair of crossing x-ray beams and diffract optical laser pulses at 400 nm from the TG. The key technical advance here is being able to independently vary the delays of the x-ray pulses. Measurements were made in 3 different solid samples: bismuth germinate (BGO), zinc oxide (ZnO) and yttrium aluminum garnet (YAG). The resulting phase-matched, 4WM signal is measured in two different ways: by varying the x-ray, x-ray pulse delay which can reveal both material and light source coherence properties and also by varying the optical laser delay with respect to the x-ray TG to study how the x-ray excitation couples to the optical properties. Although no coherent 4WM signal was seen in these measurements, the absence of this signal gives important information on experimental requirements for detecting this in future work. Also, our laser-delay scans, although not a new measurement, were applied to different materials than in past work and reveal new examples x-ray induced lattice dynamics in solids. This work represents a key step towards extending nonlinear optics and time-resolved spectroscopy into the hard x-ray regime.
High-precision turning and ultra-smooth direct polishing of aluminum alloy mirrors
Authors: Song Peng , Yang Chao , Bai Yang , Ding Jiawen , Guo Jie , Li Chuang , Wang Yuxuan , Xue Changxi
Published: August 2023

Due to the high surface roughness requirements of aluminum alloy mirrors used in the visible light band, there are still great challenges in single point diamond turning of high-surface quality aluminum alloy mirrors. In this paper, a processing method for aluminum alloy mirrors is proposed. Based on single point diamond turning technology, the prediction model of aluminum alloy surface roughness was established. The mapping relationship between the surface roughness of the aluminum alloy mirror and each turning parameter was obtained, and the maximum possible surface quality was achieved. On the basis of the turning results, the method of small tool polishing was used to remove the turning texture generated by the copy effect of the tool arc radius, suppress errors of the medium and high-frequency, and reduce the surface roughness. The single abrasive removal efficiency model was established and mechanical removal in the polishing process was analyzed. Combined with the chemical action in the polishing process, two types of polishing liquid—acidic and neutral, were prepared and analyzed. The optimal polishing parameters were obtained through multiple single-factor experiments. On the basis of this, the surface roughness of the aluminum alloy after turning was optimized. The results show that the value was reduced from 4.811 to 1.482 nm, an increase of 69.2%. This method can effectively improve the machining accuracy of aluminum alloy mirrors and provide an important process guarantee for the application of aluminum alloy materials in visible-light systems.
Single image relighting based on illumination field reconstruction
Authors: Zhang Jingyuan , Chen Xiaoyu , Tang Weining , Yu Haotian , Bai Lianfa , Han Jing
Published: August 2023

Relighting a single low-light image is a crucial and challenging task. Previous works primarily focused on brightness enhancement but neglected the differences in light and shadow variations, which leads to unsatisfactory results. Herein, an illumination field reconstruction (IFR) algorithm is proposed to address this issue by leveraging physical mechanism guidance, physical-based supervision, and data-based modeling. Firstly, we derived the Illumination field modulation equation as a physical prior to guide the network design. Next, we constructed a physical-based dataset consisting of image sequences with diverse illumination levels as supervision. Finally, we proposed the IFR neural network (IFRNet) to model the relighting progress and reconstruct photorealistic images. Extensive experiments demonstrate the effectiveness of our method on both simulated and real-world datasets, showing its generalization ability in real-world scenarios, even training solely from simulation.
Fabrication of a flexible stretchable hydrogel-based antenna using a femtosecond laser for miniaturization
Authors: Zhao Pingping , Chen Tao , Si Jinhai , Shi Hongyu , Hou Xun
Published: August 2023

We demonstrated a new method of fabricating a stretchable antenna by injecting liquid metal (LM) into a femtosecond-laser-ablated embedded hydrogel microchannel, and realized miniaturization of a stretchable dipole antenna based on hydrogel substrate. Firstly, symmetrical microchannels with two equal and linear branches were formed by a femtosecond laser in the middle of a hydrogel substrate, and then were filled with LM by use of a syringe needle. Using this method, a stretchable LM-dipole antenna with each dimension of  24 mm × 0.6 mm × 0.2 mm separated by a 2-mm gap, was formed in the middle of a 70 mm × 12 mm × 7 mm hydrogel slab. Since the polyacrylamide (PAAm) hydrogel contained ∼ 95 wt % deionized water with a high permittivity of 79 in the 0.5 GHz - 1.5 GHz range, the hydrogel used to prepare the flexible antenna can be considered as distilled water boxes. Experiments and simulations showed that a 5-cm-long LM-dipole embedded in hydrogel resonated at approximately 927.5 MHz with an S11 value of about - 12.6 dB and omnidirectional radiation direction. Benefiting from the high permittivity of the hydrogel, the dipole length was downsized by about half compared with conventional polymer substrates at the same resonant frequency. By varying the applied strain from 0 to 48%, the resonant frequency of the hydrogel/LM dipole antenna can be tuned from 770.3 MHz to 927.0 MHz. This method provides a simple and scalable technique for the design and preparation of LM-pattern microstructures in hydrogels, and has potential applications in hydrogel-based soft electronic device.
Long term observations of biomass burning aerosol over Warsaw by means of multiwavelength lidar
Authors: Janicka Lucja , Davuliene Lina , Bycenkiene Steigvile , Stachlewska Iwona S.
Published: August 2023

High quality lidar measurements of PollyXT operating at the University of Warsaw in the years 2013-2022 were analyzed to present a comprehensive optical characterization of biomass burning aerosols over Warsaw. The directions of the aerosol inflows were analyzed by dividing advection cases into four types, according to the area of origin: Western Europe, Eastern Europe, the Iberian Peninsula, and North America. It was shown that optical properties of smoke vary in each of these types, and emphasized that aerosol aging processes are important. It was found that as aerosol’s age increases, there is more backscattering and less extinction at 355 nm in relation to 532 nm. The analysis of the lidar ratio demonstrated that the main changes of the aging process were observed in the UV spectra.
Ultrafine electro-optical frequency comb based on cascade phase modulation with cyclic frequency shifting
Authors: Wang Changhe , Sun Yuxin , Zhao Qilai , Yang Changsheng , Zeng Chun , Feng Zhouming , Zhang Yu , Li Li , Zhou Kaijun , Wei Xiaoming , Yang Zhongmin , Xu Shanhui
Published: August 2023

An ultrafine electro-optical frequency comb (EOFC) with plentiful comb
teeth is demonstrated. Adopting a single-frequency fiber laser as a
light source, cascade phase modulation based on a sinusoidal signal
and a frequency-time transformation (FTT) signal is executed to
generate the EOFC with high fineness. Meanwhile, a cyclic fast
frequency shifting strategy is introduced to boost the number of comb
teeth and the bandwidth of the EOFC. As a result, an EOFC with 12600
comb lines covering a broad bandwidth from −6.3 GHz to 6.3 GHz is
established, corresponding to an ultrafine comb space of 1 MHz.
Moreover, the power fluctuation of a comb tooth is less than 0.5 dBm.
This state-of-the-art EOFC has significant potential in the field of
precision spectroscopy.
Broadly tunable continuous wave 2.3-µm Tm3+:tellurite bulk glass laser
Authors: Morova Yagız , Ardalı Emir , Denker Boris , Galagan Boris , Sverchkov Sergei , Sennaroglu Alphan
Published: August 2023

We report, for the first time to the best of our knowledge, a continuous wave trivalent thulium ion (Tm3+)-doped bulk glass near 2.3 µm. In the experiments, a bulk Tm3+-doped tellurite glass with the stoichiometric composition of 74TeO2–12ZnO–4La2O3–10Na2O (Tm3+:TZLN) was used. Lasing operation was achieved by using an x-fold cavity at the free-running wavelength of 2303 nm. The maximum slope efficiency of 6.2% was obtained with respect to the absorbed pump power with a 1% transmitting output coupler. In this case, as high as 100-mW output power was generated with 2.2 W of absorbed pump power. Continuous, broad tuning was achieved from 2233 nm to 2400 nm. The excitation spectrum of the laser was also investigated and 2.3-µm lasing was obtained by varying the pump wavelength over the 773–809-nm range. The absorption cross section was determined to be 4.4 × 10−21 cm2, based on open-aperture z-scan measurement. By using the laser efficiency data, the emission cross section of the Tm3+:TZLN glass was further determined to be 1.3 × 10−20 cm2 at 2.3 µm.
Terahertz disk resonator on a substrateless dielectric waveguide platform
Authors: Dechwechprasit Panisa , Tanyi Ako Rajour , Sriram Sharath , Fumeaux Christophe , Withayachumnankul Withawat
Published: August 2023

Resonant cavities are fundamental to and versatile for terahertz integrated systems. So far, integrated resonant cavities have been implemented in relatively lossy terahertz platforms. In this Letter, we propose a series of integrated disk resonators built into a low-loss substrateless silicon waveguide platform, where the resonances and associated quality factor (Q-factor) can be controlled via an effective medium. The measurement results demonstrate that the Q-factor can reach up to 9146 at 274.4 GHz due to the low dissipation of the platform. Additionally, these resonators show strong tunability of the resonance under moderate optical power. These terahertz integrated disk resonators can be employed in sensing and communications.
Multiple beam coherent combination via an optical ring resonator
Authors: Bode Nina , Holmes Zachary , Ng Sebastian , von Behren Benjamin , Ottaway David , Willke Benno
Published: August 2023

Future gravitational wave detectors (GWDs) require low noise, single
frequency, continuous wave lasers with excellent beam quality and
powers in excess of 500 W. Low noise laser amplifiers with high
spatial purity have been demonstrated up to 300 W. For higher powers,
coherent beam combination can overcome scaling limitations. In this
Letter we introduce a new, to the best of our knowledge, combination
scheme that uses a bow-tie resonator to combine three laser beams with
simultaneous spatial filtering performance.
Effect of noise-induced quantum coherence in the intermediate band solar cells
Authors: Daryani M. , Rostami A. , Darvish G. , Morravej Farshi M. K.
Published: August 2023

It has been shown that quantum coherence induced by incoherent light can increase the efficiency of solar cells. Here we evaluate the effect of such coherence in the intermediate band solar cells (IBSC). We first examine a six-level quantum IBSC model and demonstrate by simulation that the maximum output power in a solar cell with a quantum structure increases more than 16 percent in the case of coherence existence. We then propose an IBSC model which can absorb continuous spectra of sunlight and show that the quantum coherence can increase the output power of the cell. For instance, calculations indicate that the coherence makes an increase of about 31% in the maximum output power of a cell that the width of the conduction and intermediate bands are 100 and 10 meV, respectively. Also, our calculations show that the quantum coherence effect is still observed in increasing the solar cell power by expanding the width of the conduction band, although the output power is reduced due to an increase in thermalization loss. However, expanding the width of the intermediate band reduces the coherence effect.
All-glass miniature GHz repetition rate femtosecond laser cavity
Authors: Delgoffe Antoine , Nazir Saood , Hakobyan Sargis , Hönninger Clemens , Bellouard Yves
Published: August 2023

Free-space optics allows for design freedom and control, but
miniaturization and manufacturability are limited. Here, we present a
method for manufacturing complex miniaturized free-space optical
systems that combines contactless femtosecond laser-activated
alignment with femtosecond laser 3D manufacturing of a substrate
incorporating optomechanical elements. Specifically, we demonstrate a
palm-sized, all-glass GHz femtosecond laser cavity, whose alignment
and lasing operation are permanently tuned in a contactless manner via
laser–matter interaction using another femtosecond laser. The
manufactured Yb:KYW oscillator shows self-starting mode-locking with a
diffraction-limited beam and outputs a stable train of solitons with
182 fs pulse width at 1.0925 GHz repetition rate, for
725 mW incident pump power.
Strengthening the community governance of healthcare services in ‘fragile’ settings: Evidence from Burundi and South Kivu, DR Congo
Authors: Falisse Jean-Benoit , Nkengurutse Hugues , Ntakarutimana Léonard
Published: August 2023

Community governance, the direct (co-)management of public services by community members, is a popular approach to improve the quality of, and access to, healthcare services–including in so-called ‘fragile’ states. The effectiveness of such approach is, however, debated, and scholars and practitioners have emphasised the need to properly reflect on the contextual features that may influence social accountability interventions. We study a randomised intervention during which community-elected health facility committee members were trained on their roles and rights in the co-management of primary healthcare facilities. 328 publicly-funded health facilities of Burundi and Sud Kivu in DR Congo were followed over a period of one year. In Kivu, but not in Burundi, the intervention strengthened the position of the committee vis-à-vis the health facility nurses and affected the management of the facility. HFC members mostly focused on improving the elements most accessible to them: hiring staff and engaging in basic construction and maintenance work. Using survey data and interviews, we argue that part of the discrepancy in results between the two contexts can be explained by differences in health facilities’ management (whether they primarily depend on a local church or more distant authorities) as well as different local histories of relationship to public service providers. The former affects the room available for change, while the latter affects the relevance of the citizens’ committee as an acceptable way to interact with healthcare providers. No effect was found on the perceived quality of and access to services, and the committees, even when strengthened, appear disconnected from the citizens. The findings are an invitation to re-think the conditions under which bottom-up accountability mechanisms such as citizens committees can be effective in ‘fragile’ settings.
Adenosine triphosphate (ATP) sampling algorithm for monitoring the cleanliness of surgical instruments
Authors: Pontes Daniela Oliveira , Costa Dayane de Melo , da Silva Pereira Priscilla Perez , Whiteley Greg S. , Glasbey Trevor , Tipple Anaclara Ferreira Veiga
Published: August 2023

Background

Timely detection of cleaning failure is critical for quality assurance within Sterilising Service Units (SSUs). Rapid Adenosine Triphosphate (ATP) testing provides a real time and quantitative indication of cellular contaminants, when used to measure surface or device cleanliness. The aim of this study was to investigate the use of an ATP algorithm and to whether it could be used as a routine quality assurance step, to monitor surgical instruments cleanliness in SSUs prior to sterilisation.

Methods

Cleanliness monitoring using rapid ATP testing was undertaken in the SSUs of four hospitals located in the western (Amazonia) region of Brazil. ATP testing was conducted (Clean Trace, 3M) on 163 surgical instruments, following manual cleaning. A sampling algorithm using a duplicate swab approach was applied to indicate surgical instruments as (i) very clean, (ii) clean, (iii) equivocal or (iv) fail, based around a ‘clean’ cut-off of 250 Relative Light Units (RLU) and a ‘very clean’ <100 RLU.

Results

The four cleanliness categories were significantly differentiated (P≤0.001). The worst performing locations (hospitals A & C) had failure rates of 39.2% and 32.4%, respectively, and were distinctly different from hospitals B & D (P≤0.001). The best performing hospitals (B & D) had failure rates of 7.7% and 2.8%, respectively.

Conclusion

The ATP testing algorithm provides a simple to use method within SSUs. The measurements are in real time, quantitative and useful for risk-based quality assurance monitoring, and the tool can be used for staff training. The four-tiered approach to the grading of surgical instrument cleanliness provides a nuanced approach for continuous quality improvement within SSU than does a simple pass/fail methodology.
Research on the risk transmission mechanism of international construction projects based on complex network
Authors: Huangfu Yanjun , Xu Jingrong , Zhang Yang , Huang Dechun , Chang Jiahui
Published: August 2023

The risk transmission process between international construction projects largely contributes to the dilemma of risk management of international construction projects. Firstly, this paper adopts methods such as literature review and brainstorming to identify the risks in international construction projects from all aspects and all stages. Connections between risks is built by the Delphi method and further construct the international construction project risk network. Combined with “ucinet”, a network visualization analysis tool, overall feature parameters and local feature parameters are presented for analysis as the focus. Starting from this, the risk transmission in complex construction projects is analyzed to identify key risks and transmission relationships and reveal inherent laws of risk transmission. Accordingly, when formulating risk prevention strategies for international engineering projects, it is proposed that measures to curb risk transmission should be effectively adopted from both key risks and their transmission relationships.
Single molecule studies characterize the kinetic mechanism of tetrameric p53 binding to different native response elements
Authors: Suwita Johannes P. , Voong Calvin K. , Ly Elina , Goodrich James A. , Kugel Jennifer F.
Published: August 2023

The transcriptional activator p53 is a tumor suppressor protein that controls cellular pathways important for cell fate decisions, including cell cycle arrest, senescence, and apoptosis. It functions as a tetramer by binding to specific DNA sequences known as response elements (REs) to control transcription via interactions with co-regulatory complexes. Despite its biological importance, the mechanism by which p53 binds REs remains unclear. To address this, we have used an in vitro single molecule fluorescence approach to quantify the dynamic binding of full-length human p53 to five native REs in real time under equilibrium conditions. Our approach enabled us to quantify the oligomeric state of DNA-bound p53. We found little evidence that dimer/DNA complexes form as intermediates en route to binding or dissociation of p53 tetramer/DNA complexes. Interestingly, however, at some REs dimers can rapidly exchange from tetramer/DNA complexes. Real time kinetic measurements enabled us to determine rate constants for association and dissociation at all five REs, which revealed two kinetically distinct populations of tetrameric p53/RE complexes. For the less stable population, the rate constants for dissociation were larger at REs closest to consensus, showing that the more favorable binding sequences form the least kinetically stable complexes. Together our single molecule measurements provide new insight into mechanisms by which tetrameric p53 forms complexes on different native REs.
Revisiting food insecurity gender disparity
Authors: Silva Andres , Astorga Andres , Faundez Rodrigo , Santos Karla
Published: August 2023

Previous research has shown that woman-headed households, more than man-headed ones, experience food insecurity. The purpose of this article is to contribute on the determinants that are linked to this gender disparity. Using a nationally representative dataset from Chile, we found that food security household head gender disparity is associated with marital status (having or not a partner) and household composition (having children or seniors). In contrast, gender disparity is not strongly associated with household income and household head educational differences. In this way, we expect to bring evidence to inform new alternatives that help mitigate food security gender disparity.
Salinity and nitrogen source affect productivity and nutritional value of edible halophytes
Authors: Farzana Tania , Guo Qi , Rahman Md. Sydur , Rose Terry J. , Barkla Bronwyn J.
Published: August 2023

Saline agriculture may contribute to food production in the face of the declining availability of fresh water and an expanding area of salinized soils worldwide. However, there is currently little known about the biomass and nutrient/antinutrient accumulation response of many edible halophytes to increasing levels of salinity and nitrogen source. To address this, two glass house experiments were carried out. The first to study the shoot biomass, and nutrient accumulation response, measured by ICP-MS analysis, of edible halophyte species, including Mesembryanthemum crystallinum (ice plant), Salsola komarovii (Land seaweed), Enchylaena tomentosa (Ruby Saltbush), Crithmum maritimum (Rock Samphire), Crambe maritima (Sea Kale) and Mertensia maritima (Oyster Plant), under increasing levels of salinity (0 to 800 mM). The second experiment studied the effects of nitrogen source combined with salinity, on levels of oxalate, measured by HPLC, in ice plant and ruby saltbush. Species differences for biomass and sodium (Na), potassium (K), chloride (Cl), nitrogen (N) and phosphorus (P) accumulation were observed across the range of salt treatments (0 to 800mM). Shoot concentrations of the anti-nutrient oxalate decreased significantly in ice plant and ruby saltbush with an increase in the proportion of N provided as NH4+ (up to 100%), while shoot oxalate concentrations in ice plant and ruby saltbush grown in the absence of NaCl were not significantly different to oxalate concentrations in plants treated with 200 mM or 400 mM NaCl. However, the lower shoot oxalate concentrations observed with the increase in NH4+ came with concurrent reductions in shoot biomass. Results suggest that there will need to be a calculated tradeoff between oxalate levels and biomass when growing these plants for commercial purposes.
Trialling an optimised social groups intervention in services to enhance social connectedness and mental health in vulnerable young people (TOGETHER): Study protocol for a feasibility randomised controlled trial
Authors: Vella Claire , Berry Clio , Easterbrook Matthew J. , Bibby-Jones Anna-Marie , Michelson Daniel , Bogen-Johnston Leanne , Fowler David
Published: August 2023

Background

Calls have been made to rethink the mental health support currently available for young people. This study aims to help re-focus and reduce the inaccessibility of mental health services by offering an adapted version of a theoretically-driven, evidence-based, guided psychosocial intervention known as ‘Groups 4 Health’ (G4H). To date, the G4H intervention has mainly been trialled in Australia, with promising positive effects on social connection, mental health and well-being. The present study examines the feasibility of running a randomised controlled trial when delivering the G4H intervention for young people in the UK.

Methods

The TOGETHER study is a feasibility randomised controlled trial of an adapted version of the G4H intervention. Participants are aged 16–25, currently experiencing mental health difficulties and recruited from mental health services. The target sample size is 30, with 15 in each trial arm. Participants are randomly allocated to either G4H plus treatment as usual, or treatment as usual alone. The primary outcomes of interest are the feasibility of recruitment, randomisation, data collection and retention to the study at 10 and 14 week follow up, as well as the acceptability, and accessibility of the study protocol and G4H intervention.

Discussion

The results of this study will indicate if further optimisation is required to improve the feasibility, acceptability and accessibility of the intervention and study protocol procedures as perceived by end users and practitioners. This offers a significant opportunity to support the local and national demand for accessible, innovative, and effective psychosocial youth mental health support.

Trial registration

ISRCTN registry (ISRCTN12505807). Registration date: 11/04/2022.
Disciplinary trends in the use of the Delphi method: A bibliometric analysis
Authors: Khodyakov Dmitry , Grant Sean , Kroger Jack , Gadwah-Meaden Catria , Motala Aneesa , Larkin Jody
Published: August 2023

The Delphi method is an iterative, anonymous, group-based process for eliciting and aggregating opinion on a topic to explore the existence of consensus among experts. The year 2023 marks the 60th anniversary of the first peer-reviewed journal article on the Delphi method. Originally developed for operations research, this method is now applied extensively by researchers representing diverse scientific fields. We used a bibliometric analysis to describe general trends in the expansion of its use across disciplines over time. We conducted a systematic literature search for all English-language, peer-reviewed journal articles on the Delphi method through its first 60 years. We found 19,831 articles: 96.8% (n = 19,204) on the actual use of the Delphi method in an empirical study and 3.2% (n = 627) describing, examining, or providing some guidance on how to use the Delphi method. Almost half (49.9%) of all articles were published in the 2010s and an additional third (32.5%) in the first few years of the 2020s. Nearly two-thirds (65%, n = 12,883) of all published articles have appeared in medical journals, compared to 15% in science and technology (n = 3,053) or social science (n = 3,016) journals. We conclude that the expanded use of the Delphi method has been driven largely by the medical field, though social scientists and technologists continue to be at the forefront of methodological work on the Delphi method. Therefore, we call for greater transdisciplinary collaboration on methodological guidance and standards for the Delphi method.
What determines the performance of low-carbon cities in China? Analysis of the grouping based on the technology—Organization—Environment framework
Authors: Chen Weidong , Cai Quanling , Di Kaisheng , Li Dongli , Liu Caiping , Wang Mingxing , Liu Sichen , Di Zhensheng , Shi Qiumei
Published: August 2023

Background and objectives

Addressing climate change and reducing greenhouse gas emissions have emerged as shared global objectives. Enhancing the development performance of low-carbon cities has become an urgent and widely acknowledged concern for both government policy-making departments and academics. Drawing upon the complex grouping perspective and resource allocation theory, this study investigates how varying conditions related to technology, organization, and environment in Chinese low-carbon pilot cities can effectively allocate resources to shape the governance performance of low-carbon cities.

Methods and data

This paper employs a comprehensive grouping analysis perspective, treating the research object as a combination of various ways between condition variables. It integrates the advantages of case studies and variable studies, and investigates the collective relationships between elemental groupings and outcomes using the fsQCA analysis method. This approach facilitates the understanding of multiple concurrent causal relationships within the technology-organization-environment (TOE) framework, accounting for different performance levels in Chinese low-carbon pilot cities, as well as addressing complex causal issues such as asymmetry and multiple scenario equivalence. Data from 30 representative low-carbon pilot cities in China were employed to validate the TOE theoretical framework.

Conclusion

No single element alone can be considered a necessary condition for low-carbon city governance performance. However, environmental enhancement plays a more prominent role in the governance performance of low-carbon cities. Additionally, the presence of "multiple concurrent" technical, organizational, and environmental conditions leads to a diverse range of governance performance in Chinese low-carbon pilot cities. In other words, the driving paths of low-carbon city performance exhibit distinct pathways.

Contribution

The findings of this study can assist low-carbon pilot city managers in generating effective governance ideas, facilitating the successful implementation of low-carbon city pilot projects, and drawing valuable lessons from the experience of low-carbon city development in China.
Urinary titin is not an early biomarker of skeletal muscle atrophy induced by muscle denervation in mice
Authors: Tanihata Jun , Minamisawa Susumu
Published: August 2023

Early detection of skeletal muscle atrophy is important to prevent further muscle weakness. However, there are few non-invasive biomarkers for skeletal muscle atrophy. Recent studies have reported that the N-terminal fragment (N-titin) of titin, a giant sarcomeric protein, is detected in the urine of patients with muscle damage. In this study, we hypothesized that urinary N-titin would be a potential early biomarker of skeletal muscle atrophy in mice caused by sciatic nerve denervation. Male mice were randomly divided into control and denervation groups, and urinary N-titin levels were assessed daily for 9 days using an enzyme-linked immunosorbent assay system. Despite reduced titin protein levels in atrophic muscles 10 days after denervation, cleaved N-titin fragments were not increased in the urine of mice with denervation-induced muscle atrophy. Furthermore, we found no uptake of Evans blue dye from the extracellular space into the cytoplasm in atrophic muscles, suggesting that the sarcomeric membrane is intact in those muscles. The present results suggest that cleaved N-titin in the urine is not suitable as an early biomarker of skeletal muscle atrophy.
Predictors of hospital admission when presenting with acute-on-chronic breathlessness: Binary logistic regression
Authors: Hutchinson Ann , Pickering Alastair , Williams Paul , Johnson Miriam
Published: August 2023

Background

Breathlessness due to medical conditions commonly causes emergency department presentations and unplanned admissions. Acute-on-chronic breathlessness is a reason for 20% of emergency presentations by ambulance with 69% of these being admitted. The emergency department may be inappropriate for many presenting with acute-on-chronic breathlessness.

Aim

To examine predictors of emergency department departure status in people with acute-on-chronic breathlessness.

Design, setting and method

Secondary analysis of patient-report survey and clinical record data from consecutive eligible attendees by ambulance. Variables associated with emergency department departure status (unifactorial analyses; p<0.05) were included in a binary logistic regression model. The study was conducted in a single tertiary hospital. Consecutive survey participants presenting in May 2015 with capacity were eligible. 1,212/1,345 surveys were completed. 245/1,212 presented with acute-on-chronic breathlessness, 171 of whom consented to clinical record review and were included in this analysis.

Results

In the final model, the odds of admission were increased with every extra year of age [OR 1.041 (95% CI: 1.016 to 1.066)], having talked to a specialist doctor about breathlessness [9.262 (1.066 to 80.491)] and having a known history of a heart condition [4.177 (1.680 to 10.386)]. Odds of admission were decreased with every percentage increase in oxygen saturation [0.826 (0.701 to 0.974)].

Conclusion

Older age, lower oxygen saturation, having talked to a specialist, and having history of a cardiac condition predict hospital admission in people presenting to the emergency department with acute-on-chronic breathlessness. These clinical factors could be assessed in the community and may inform the decision regarding conveyance.
Mental health and suicide risk among homosexual males in Bangladesh
Authors: Mozumder Muhammad Kamruzzaman , Jasmine Umme Habiba , Haque Md. Ashikul , Haque Shamsul
Published: August 2023

Background

Homosexual individuals are at high risk of suicide, but there is a shortage of data from developing countries to confirm this. Estimates on mental health and suicide risk among male homosexuals in Bangladesh are needed to generate awareness and to plan services accordingly.

Method

We assessed mental health and suicidal behavior of 102 self-identified homosexual males from a community-based organization that works with the sexual minority population.

Results

One-third of the participants (32.4%) had experienced attempted suicide, and almost half (47.1%) had a history of suicidal ideation and self-harm (40.2%). Compared to a heterosexual sample, homosexual males had poorer mental health as they scored higher on Beck Hopelessness Scale (Cohen’s d = 0.29) and General Health Questionnaire (GHQ) (Cohen’s d = 0.57). The results revealed positive correlations between self-harm, suicidal ideation and suicide attempt scores. Participants with history of suicide attempt, suicide ideation and self-harm reported worse general health, more social dysfunction, and severe depression than those without such history.

Conclusion

Suicidality and mental health conditions among homosexual males in Bangladesh have appeared to be alarming. Given the concerns, we offer some recommendations for practitioners and social workers who are serving this population in Bangladesh.
Nonlinear shear characteristics of frozen loess-concrete interface
Authors: Zhang Qingwei , Zhang Chunguang
Published: August 2023

Under the different temperature environment, the precast pile-soil interface characteristics has an important impact on the safety and long-term stability for pile foundation. A large precast pile-soil shear experimental device is used to carry out the direct shear test of concrete-loess interface with different moisture contents under different freezing temperatures. The variation laws of shear strength parameters are revealed with influencing factors, and the shear mechanism of interface is discussed. The stress-strain constitutive equation of interface is proposed, and the shear strength criterion is established with considering the effects of temperature and moisture content on cohesion and internal friction angle. The results show the curve of shear stress and shear displacement can be divided into three stages: elastic deformation stage, plastic deformation stage and sliding failure stage, which macroscopically reflects the shear failure mechanism of the frozen soil-concrete interface. The shear strength of the interface is affected by the test temperature, sample moisture content and normal stress. The lower the test temperature, the greater the shear strength of the interface; With the increase of normal stress, the shear strength of interface increases; With the increase of moisture content, the shear strength of the interface increases and then decreases. The relationship of shear stress and shear displacement of frozen soil-concrete interface can be well described by the piecewise combination of hyperbolic function and linear function.
PAXIP1-AS1 is associated with immune infiltration and predicts poor prognosis in ovarian cancer
Authors: Chen Buze , Lu Xiaoyuan , Zhou Qingmei , Chen Qing , Zhu Siyan , Li Guilin , Liu Hui
Published: August 2023

The long non-coding RNA (LncRNA) PAXIP1 antisense RNA 1 (PAXIP1-AS1) was found to promote proliferation, migration, EMT, and apoptosis of ovarian cancer (OC) cells in OC cell lines, but the relationship between PAXIP1-AS1 expression and clinical characteristics, prognosis, and immune infiltration of OC patients and its regulatory network are unclear. 379 OC tissues were collected from The Cancer Genome Atlas (TCGA) database. 427 OC tissues and 88 normal ovarian tissues were collected from GTEx combined TCGA database. 130 OC samples were collected from GSE138866. Kruskal-Wallis test, Wilcoxon sign-rank test, logistic regression, Kaplan-Meier method, Cox regression analysis, Gene set enrichment analysis (GSEA), and immuno-infiltration analysis were used to evaluate the relationship between clinical characteristics and PAXIP1-AS1 expression, prognostic factors, and determine the significant involvement of PAXIP1-AS1 in function. QRT-PCR was used to validate the expression of PAXIP1-AS1 in OC cell lines. Low PAXIP1-AS1 expression in OC was associated with age (P = 0.045), histological grade (P = 0.011), and lymphatic invasion (P = 0.004). Low PAXIP1-AS1 expression predicted a poorer overall survival (OS) (HR: 0.71; 95% CI: 0.55–0.92; P = 0.009), progression free interval (PFS) (HR: 1.776; 95% CI: 1.067–2.955; P = 0.001) and disease specific survival (DSS) (HR: 0.67; 95% CI: 0.51–0.89; P = 0.006). PAXIP1-AS1 expression (HR: 0.711; 95% CI: 0.542–0.934; P = 0.014) was independently correlated with PFS in OC patients. GSEA demonstrated that neutrophil degranulation, signaling by Interleukins, GPCR-ligand binding, G alpha I signaling events, VEGFAVEGFR-2 signaling pathway, naba secreted factors, Class A 1 Rhodopsin-Like Receptors, PI3K-Akt signaling pathway, and Focal Adhesion-PI3K-Akt-mTOR-signaling pathway were differentially enriched in PAXIP1-AS1 high expression phenotype. PAXIP1-AS1 was significantly downregulated in OC cell lines compared with IOSE29 cell line. The expression of PAXIP1-AS1 was associated with immune infiltration. low expression of PAXIP1-AS1 was correlated with poor OS (HR: 0.52; 95% CI: 0.34–0.80; P = 0.003) from GSE138866. There were some genomic variations between the PAXIP1-AS1 high and low expression groups. Low expression of PAXIP1-AS1 was significantly associated with poor survival and immune infiltration in OC. PAXIP1-AS1 could be a promising prognosis biomarker and response to immunotherapy for OC.
Genome taxonomy of the genus Neptuniibacter and proposal of Neptuniibacter victor sp. nov. isolated from sea cucumber larvae
Authors: Kudo Rika , Yamano Ryota , Yu Juanwen , Koike Shotaro , Haditomo Alfabetian Harjuno Condro , de Freitas Mayanne A. M. , Tsuchiya Jiro , Mino Sayaka , Thompson Fabiano , Romalde Jesús L. , Kasai Hisae , Sakai Yuichi , Sawabe Tomoo
Published: August 2023

A Gram-staining-negative, oxidase-positive, strictly aerobic rod-shaped bacterium, designated strain PT1T, was isolated from the laboratory-reared larvae of the sea cucumber Apostichopus japonicus. A phylogenetic analysis based on the 16S rRNA gene nucleotide sequences revealed that PT1T was closely related to Neptuniibacter marinus ATR 1.1T (= CECT 8938T = DSM 100783T) and Neptuniibacter caesariensis MED92T (= CECT 7075T = CCUG 52065T) showing 98.2% and 98.1% sequence similarity, respectively. However, the average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values among these three strains were 72.0%-74.8% and 18.3%-19.5% among related Neptuniibacter species, which were below 95% and 70%, respectively, confirming the novel status of PT1T. The average amino acid identity (AAI) values of PT1T showing 74–77% among those strains indicated PT1T is a new species in the genus Neptuniibacter. Based on the genome-based taxonomic approach, Neptuniibacter victor sp. nov. is proposed for PT1T. The type strain is PT1T (JCM 35563T = LMG 32868T).
Novel competitive enzyme-linked immunosorbent assay for the detection of the high-risk Human Papillomavirus 18 E6 oncoprotein
Authors: Contreras Natalia E. , Roldán Julieta S. , Castillo Daniela S.
Published: August 2023

Cervical cancer represents a global concern with 604,000 new cases and 342,000 deaths reported annually, with the vast majority diagnosed in low income countries. Despite high-risk Human Papillomavirus (HR HPV)-induced cervical cancer has become highly preventable through prophylactic vaccines, screening programs are critical in the control of cervical carcinogenesis in populations with limited access to vaccination and in older generations of women who have already been exposed to HR HPV infection. The surge of HPV molecular tests has provided a more sensitive and accurate diagnostic alternative to cytology screening. Given that HPV DNA testing presents a low positive predicted value, leading to unnecessary treatment, the E6 oncoprotein from HR HPV types arises as a promising diagnostic marker for its overexpression in transformed HPV-positive cancer cells. For these reasons, this study aimed at obtaining monoclonal antibodies (mAbs) against the E6 oncoprotein of one of the most prevalent HR HPV types worldwide, HPV18, in order to develop a highly specific and sensitive indirect competitive ELISA (icELISA). The production of hybridomas secreting HPV18 E6 mAbs was carried out through a combined tolerization and immunization strategy, in order to avoid cross-reactivity with the E6 protein from low-risk HPV types 6 and 11. We selected the 7D2 hybridoma clone, which recognized HPV18 E6 and showed some cross-reactivity against the HR HPV45 E6 oncoprotein. The 7D2 mAb enabled the development of a sensitive, reliable and reproducible icELISA to detect and quantify small amounts of HPV18 E6 biomarker for cervical cancer progression. The present study establishes a valid 7D2-based icELISA that constitutes a promising bioanalytical method for the early detection and quantification of HPV18 E6 oncoprotein in cervical swab samples and cancer prevention.
Persistent pain management in an oncology population through pain neuroscience education, a multimodal program: PaiNEd randomized clinical trial protocol
Authors: Fernández-Gualda Miguel Ángel , Ariza-Vega Patrocinio , Lozano-Lozano Mario , Cantarero-Villanueva Irene , Martín-Martín Lydia , Castro-Martín Eduardo , Arroyo-Morales Manuel , Tovar-Martín Isabel , Lopez-Garzon Maria , Postigo-Martin Paula , González-Santos Ángela , Artacho-Cordón Francisco , Ortiz-Comino Lucía , Galiano-Castillo Noelia , Fernández-Lao Carolina
Published: August 2023

Introduction

Pain is one of the most persistent symptoms after cancer treatment. The central nervous system can erroneously stay in its alarm phase, altering the pain experience of patients who have cancer. Pain neuroscience education (PNE) with multimodal approaches may benefit these patients.

Objective

This protocol aims to determine the effectiveness of a PNE tool on pain, physical function and quality of life, as a supplement to a multimodal rehabilitation (MR) program in patients who had breast cancer (BC).

Methods

An 8-week double-blinded randomized controlled trial will be conducted, including 72 participants who had BC and who have persistent pain, randomized into three groups: PNE program + MR program, traditional biomedical information + MR program and control group. The PNE program will include educational content that participants will learn through a mobile app and the MR program will include a concurrent exercise program and manual therapy. The primary outcome will be the perceived pain assessed using the Visual Analogue Scale and secondary outcomes are others related to pain, physical function and quality of life. All outcomes will be evaluated at baseline, at the end of the intervention and 6 months after the end of intervention.

Discussion

The proposed study may help BC patients with persistent pain improve their pain experience, quality of life and provide for more adaptive pain-coping strategies. This protocol could propose an action guide to implement different integral approaches for the treatment of sequelae. This treatment option could be offered to this patient profile and it could be easily implemented in the healthcare systems due to its low costs.

Trial registration

ClinicalTrials.gov, NCT04877860. (February18, 2022).
Can endophytic microbial compositions in cane roots be shaped by different propagation methods
Authors: Yang Da , Lin Xinru , Wei Yufei , Li Zujian , Zhang Haodong , Liang Tian , Yang Shangdong , Tan Hongwei
Published: August 2023

In practical production, cane stems with buds are generally used as seed for propagation. However, long-terms cane stems only easily lead to some problems such as disease sensitivity, quality loss, etc. Recently, cane seedings, which are produced by tissue culture were used in sugarcane production, but few studies on cane health related to tissue culture seedings. Therefore, to evaluate the immunity and health of sugarcanes growing from different reproduction modes, the endophytic microbial compositions in cane roots between stem and tissue culture seedlings were analyzed using high-throughput techniques. The results showed that the endophytic microbial compositions in cane roots were significant differences between stem and tissue culture seedlings. At the genus level, Pantoea, Bacillus, Streptomyces, Lechevalieria, Pseudomonas, Nocardioides, unclassified_f__Comamonadaceae enriched as the dominant endophytic bacterial genera, and Rhizoctonia, Sarocladium, Scytalidium, Wongia, Fusarium, unclassified_f__Phaeosphaer, unclassified_c__Sordariom, unclassified_f__Stachybot, Poaceascoma, Microdochium, Arnium, Echria, Mycena and Exophiala enriched as the dominant endophytic fungal genera in cane roots growing from the tissue culture seedlings. In contrast, Mycobacterium, Massilia, Ralstonia, unclassified_f__Pseudonocardiacea, norank_f__Micropepsaceae, Leptothrix and Bryobacter were the dominant endophytic bacterial genera, and unclassified_k__Fungi, unclassified_f__Marasmiaceae, Talaromyces, unclassified_c__Sordariomycetes and Trichocladium were the dominant endophytic fungal genera in cane roots growing from stem seedlings. Additionally, the numbers of bacterial and fungal operational taxonomic units (OTUs) in cane roots growing from tissue culture seedlings were significantly higher than those of stem seedlings. It indicates that not only the endophytic microbial compositions in cane roots can be shaped by different propagation methods, but also the stress resistance of sugarcanes can be improved by the tissue culture propagation method.