bluuebunny/crossref_metadata_2025 huggingface dataset:
Single-Cell Sequencing of the Healthy and Diseased Heart Reveals Cytoskeleton-Associated Protein 4 as a New Modulator of Fibroblasts Activation
Authors: Gladka Monika M. , Molenaar Bas , de Ruiter Hesther , van der Elst Stefan , Tsui Hoyee , Versteeg Danielle , Lacraz Grègory P.A. , Huibers Manon M.H. , van Oudenaarden Alexander , van Rooij Eva
Published: January 2018

Background:

Genome-wide transcriptome analysis has greatly advanced our understanding of the regulatory networks underlying basic cardiac biology and mechanisms driving disease. However, so far, the resolution of studying gene expression patterns in the adult heart has been limited to the level of extracts from whole tissues. The use of tissue homogenates inherently causes the loss of any information on cellular origin or cell type-specific changes in gene expression. Recent developments in RNA amplification strategies provide a unique opportunity to use small amounts of input RNA for genome-wide sequencing of single cells.

Methods:

Here, we present a method to obtain high-quality RNA from digested cardiac tissue from adult mice for automated single-cell sequencing of both the healthy and diseased heart.

Results:

After optimization, we were able to perform single-cell sequencing on adult cardiac tissue under both homeostatic conditions and after ischemic injury. Clustering analysis based on differential gene expression unveiled known and novel markers of all main cardiac cell types. Based on differential gene expression, we could identify multiple subpopulations within a certain cell type. Furthermore, applying single-cell sequencing on both the healthy and injured heart indicated the presence of disease-specific cell subpopulations. As such, we identified cytoskeleton-associated protein 4 as a novel marker for activated fibroblasts that positively correlates with known myofibroblast markers in both mouse and human cardiac tissue. Cytoskeleton-associated protein 4 inhibition in activated fibroblasts treated with transforming growth factor β triggered a greater increase in the expression of genes related to activated fibroblasts compared with control, suggesting a role of cytoskeleton-associated protein 4 in modulating fibroblast activation in the injured heart.

Conclusions:

Single-cell sequencing on both the healthy and diseased adult heart allows us to study transcriptomic differences between cardiac cells, as well as cell type-specific changes in gene expression during cardiac disease. This new approach provides a wealth of novel insights into molecular changes that underlie the cellular processes relevant for cardiac biology and pathophysiology. Applying this technology could lead to the discovery of new therapeutic targets relevant for heart disease.
A Universal Standard for the Validation of Blood Pressure Measuring Devices
Authors: Stergiou George S. , Alpert Bruce , Mieke Stephan , Asmar Roland , Atkins Neil , Eckert Siegfried , Frick Gerhard , Friedman Bruce , Graßl Thomas , Ichikawa Tsutomu , Ioannidis John P. , Lacy Peter , McManus Richard , Murray Alan , Myers Martin , Palatini Paolo , Parati Gianfranco , Quinn David , Sarkis Josh , Shennan Andrew , Usuda Takashi , Wang Jiguang , Wu Colin O. , O’Brien Eoin
Published: January 2018

In the past 30 years, several organizations, such as the US Association for the Advancement of Medical Instrumentation (AAMI), the British Hypertension Society, the European Society of Hypertension (ESH) Working Group on Blood Pressure (BP) Monitoring, and the International Organization for Standardization (ISO), have developed protocols for clinical validation of BP measuring devices. However, it is recognized that science, as well as patients, consumers, and manufacturers, would be best served if all BP measuring devices were assessed for accuracy according to an agreed single validation protocol that had global acceptance. Therefore, an international initiative was taken by the AAMI, ESH, and ISO experts who agreed to develop a universal standard for device validation. This statement presents the key aspects of a validation procedure, which were agreed by the AAMI, ESH, and ISO representatives as the basis for a single universal validation protocol. As soon as the AAMI/ESH/ISO standard is fully developed, this will be regarded as the single universal standard and will replace all other previous standards/protocols.
Inhibition of 5‐Hydroxytryptamine Receptor 2B Reduced Vascular Restenosis and Mitigated the β‐Arrestin2–Mammalian Target of Rapamycin/p70S6K Pathway
Authors: Liu Yahan , Wang Zhipeng , Li Jing , Ban Yiqian , Mao Guangmei , Zhang Man , Wang Mo , Liu Yan , Zhao Beilei , Shen Qiang , Xu Qingbo , Wang Nanping
Published: January 2018

Background

As a monoamine neurotransmitter, 5‐hydroxytryptamine (5‐HT) or serotonin modulates mood, appetite, and sleep. Besides, 5‐HT also has important peripheral functions. 5‐HT receptor 2B (5‐HT2BR) plays a key role in cardiovascular diseases, such as pulmonary arterial hypertension and cardiac valve disease. Percutaneous intervention has been used to restore blood flow in occlusive vascular disease. However, restenosis remains a significant problem. Herein, we investigated the role of 5‐HT2BR in neointimal hyperplasia, a key pathological process in restenosis.

Methods and Results

The expression of 5‐HT2BR was upregulated in wire‐injured mouse femoral arteries. In addition, BW723C86, a selective 5‐HT2BR agonist, promoted the injury response during restenosis. 5‐HT and BW723C86 stimulated migration and proliferation of rat aortic smooth muscle cells. Conversely, LY272015, a selective antagonist, attenuated the 5‐HT–induced smooth muscle cell migration and proliferation. In vitro study showed that the promigratory effects of 5‐HT2BR were mediated through the activation of mammalian target of rapamycin (mTOR)/p70S6K signaling in a β‐arrestin2–dependent manner. Inhibition of mammalian target of rapamycin or p70S6K mitigated 5‐HT2BR–mediated smooth muscle cell migration. Mice with deficiency of
5‐HT2BR
showed significantly reduced neointimal formation in wire‐injured arteries.

Conclusions

These results demonstrated that activation of 5‐HT2BR and β‐arrestin2–biased downstream signaling are key pathological processes in neointimal formation, and 5‐HT2BR may be a potential target for the therapeutic intervention of vascular restenosis.
Inhibiting Integrin α5 Cytoplasmic Domain Signaling Reduces Atherosclerosis and Promotes Arteriogenesis
Authors: Budatha Madhusudhan , Zhang Jiasheng , Zhuang Zhen W. , Yun Sanguk , Dahlman James E. , Anderson Daniel G. , Schwartz Martin A.
Published: January 2018

Background

Fibronectin in endothelial basement membranes promotes endothelial inflammatory activation and atherosclerosis but also promotes plaque stability and vascular remodeling. The fibronectin receptor α5 subunit is proinflammatory through binding to and activating phosphodiesterase 4D5, which inhibits anti‐inflammatory cyclic adenosine monophosphate and protein kinase A. Replacing the α5 cytoplasmic domain with that of α2 resulted in smaller atherosclerotic plaques. Here, we further assessed plaque phenotype and compensatory vascular remodeling in this model.

Methods and Results

α5/2 mice in the hyperlipidemic apolipoprotein E null background had smaller plaques in the aortic root, with reduced endothelial
NF
‐κB activation and inflammatory gene expression, reduced leukocyte content, and much lower metalloproteinase expression. However, smooth muscle cell content, fibrous cap thickness, and fibrillar collagen were unchanged, indicating no shift toward vulnerability. In vivo knockdown of phosphodiesterase 4D5 also decreased endothelial inflammatory activation and atherosclerotic plaque size. α5/2 mice showed improved recovery from hindlimb ischemia after femoral artery ligation.

Conclusions

Blocking the fibronectin‐Integrin α5 pathway reduces atherosclerotic plaque size, maintains plaque stability, and improves compensatory remodeling. This pathway is therefore a potential therapeutic target for treatment of atherosclerosis.
The Right to Custodial Legal Assistance in Europe: In Search for the Rationales
Authors: Pivaty Anna
Published: January 2018

Following national transposition of procedural rights’ Directives, various eu and domestic authorities would need to interpret their provisions, including those concerning the right to custodial legal assistance. To inform this interpretation, account must be taken of the relevant ECtHR case law. However, many of the Directives’ provisions are so unclear, and the ECtHR approach is so inconsistent, that to achieve a coherent interpretation, it appears necessary to first identify the principles behind this right. This article examines these principles, in the form of rationales, from the theoretical, jurisprudential, and eu law perspectives. It concludes that the right should be conceived as grounded in a range of rationales, notwithstanding an alternative view that it serves (only) to protect the privilege against self-incrimination. The article fleshes out the consequences of this conclusion for interpreting some of the relevant Directives’ provisions, and for criminal defence practice.
First finding of anchialine and submarine cave dwelling brittle stars from the Pacific Ocean, with descriptions of new species of Ophiolepis and Ophiozonella (Echinodermata: Ophiuroidea: Amphilepidida)
Authors: OKANISHI MASANORI , FUJITA YOSHIHISA
Published: January 2018

Two new species, Ophiolepis cavitata n. sp. and Ophiozonella cavernalis n. sp., are described from the specimens collected in shallow water submarine caves in the Ryukyu Islands, southwestern Japan. Ophiolepis cavitata n. sp. is distinguished from the other congeners in having prominent tubercle-shaped knobs on dorsal arm plates; two sizes of disc scales on the aboral disc, the larger surrounded by 3–8 times smaller disc scales; larger scales concave; radial shields wider than long; a trio of distinct scales present distal to each pair of radial shields; oral shields partly concave; 3 arm spines on proximal portion of the arms. Ophiozonella cavernalis n. sp. is distinguished from the other congeners in having polygonal, uniform size disc scales; oral shields almost as long as wide, shallow concave proximal sides that end in a fairly sharp point medially; arms about 9 times longer than disc diameter; 3 cylindrical arm spines, of which the oral most one is slightly larger than other 2 spines. Ophiozonella cavernalis n. sp. is the second finding of an anchialine-endemic ophiuroid and the first finding from the Pacific Ocean.
Morphological convergences in Ameles Burmeister and Pseudoyersinia Kirby: Taxonomic implications of wing reduction and flight predisposition in some West-Mediterranean Amelini (Insecta: Mantodea)
Authors: BATTISTON ROBERTO , CORREAS JOSÉ , LOMBARDO FRANCESCO , MOUNA MOHAMED , PAYNE KEITH , SCHÜTTE KAI
Published: January 2018

Species in the genus Ameles Burmeister and Pseudoyersinia Kirby (Amelini) are traditionally defined as small-sized, ground-dwelling mantids whose males are distinct for being, respectively, macropterous and brachypterous. However, comparative morphological studies across Amelini confirmed the existence of short-winged males in Ameles, suggesting that this traditional diagnostic concept does not apply to all species. Our analyses of several species from West Mediterranean localities (Canary Islands, Spain, Italy, and Morocco) resulted in the relocation of Pseudoyersinia andreae Galvagni, 1976 to Ameles as Ameles andreae (Galvagni, 1976) (n. comb.) with Ameles insularis Agabiti, Ippolito & Lombardo, 2010 as its new synonym (n. syn.), the clarification of the taxonomic identity of A. gracilis (Brullé, 1838) and A. maroccana Uvarov, 1931, including diagnoses of their males, and the description of Ameles spallanzania obscura (n. ssp.) (from Spain). We also take the opportunity to describe Pseudoyersinia maroccana (n. sp.) (from Morocco) based on museum specimens separating it from Ameles maroccana Uvarov, 1931. We also found that wing length is positively correlated to ocelli size. We discuss this trend from an ecological, evolutionary, and biogeographic perspectives to both facilitate species circumscription and justify the taxonomic modifications herein introduced.
Germalna, a new genus for the New Caledonian cicada previously assigned to the genus Melampsalta Kolenati, plus a complement to the description of the genus Rouxalna Boulard, with the description of two new species (Insecta: Hemiptera, Cicadoidea, Cicadidae)
Authors: DELORME QUENTIN
Published: January 2018

Species previously assigned to Melampsalta Kolenati, in New Caledonia are reviewed. Morphological studies indicate that New Caledonian cicadas currently placed in this genus have been wrongly assigned and should be placed in a new genus. The genus Germalna gen. nov., is therefore erected to accommodate Germalna germaini comb. nov. The genus Germalna gen. nov. was first documented by Michel Boulard, but remained a nomen nudum until now. A redescription of the genus Rouxalna Boulard is provided and the following new species are described: Rouxalna villosa sp. nov., and Rouxalna scabens sp. nov. Male calling songs of Rouxalna rouxi Boulard and Rouxalna scabens sp. nov. are analysed and described from field recordings. A key to the species of Rouxalna is also provided.
Taxonomic notes on the genera Scatella and Scatophila (Diptera: Ephydridae) with a remark on Trixoscelis chilensis (Trixoscelididae)
Authors: ZATWARNICKI TADEUSZ , IRWIN ANTHONY G.
Published: January 2018

Two new combinations are proposed in Scatella for Ephydra australis Walker, 1853 and Opomyza guttata Macquart, 1843. Two species are resurrected from synonymy: Scatella lacustris (Meigen, 1830), revised status and Trixoscelis chilensis (Schiner, 1868), revised status (Trixoscelididae). Nine new synonyms are suggested: Scatella insularis Mathis & Wirth, 1981 = S. australis (Walker, 1853); S. lutosa nigripes Oldenberg, 1923 = S. obsoleta Loew, 1861; S. septempunctata Malloch, 1933 = S. gratiellae Canzoneri & Raffone, 1987 = S. septemfenestrata Lamb, 1912; S. tenuicosta Collin, 1930 = S. lacustris (Meigen, 1830); S. vulgata Cresson, 1931 = S. guttata (Macquart, 1843); Scatophila zlobini Krivosheina, 2009 = S. hirtirostris Sturtevant and Wheeler, 1954; S. grisescens Frey, 1915 = S. mesogramma (Loew, 1869); S. planiceps (Boheman, 1853) = S. quadriguttata (Meigen, 1830).
Dexoris ruzzieri sp. nov. (Coleoptera: Lycidae), a new net-winged beetle from Ivory Coast
Authors: BOCAK LADISLAV
Published: January 2018

Dexoris ruzzieri sp. nov. is described from Mt. Tonkoul, Ivory Coast. This is the second record of a Dexoris species from the regions west of the Dahomey Gap. The unique specimen is available due to rareness of Dexoris Waterhouse, 1878. All Dexoris, including D. ruzzieri sp. nov., are only known from male specimens and despite the growing number of known species, females remain unknown.
Development of a Comprehensive Quality-of-Life Measure for Facial and Torso Acne
Authors: McLellan Chelsea , Frey Marc P. , Thiboutot Diane , Layton Alison , Chren Mary-Margaret , Tan Jerry
Published: January 2018

Background

Acne is a chronic skin disorder which generally presents in adolescence but continues into adulthood, and negatively affects both physical and psychosocial well-being. Presently, there are no validated acne-specific quality-of-life (QoL) measures that include dimensions for both facial and torso acne.

Objective:

The objective of this study was to develop a QoL instrument for both facial and torso acne (CompAQ) in accordance with recommended standards.

Methods:

A literature review and Delphi survey of patients and clinicians were used to develop the conceptual framework for outcomes perceived important to acne patients. An initial version of the measure was developed, CompAQ-v1, and pilot tested with patients via cognitive interviews.

Results:

The Delphi survey generated 4 domains (physical, psychological, sociological, and treatment) and 54 items. These, along with a literature review and input from clinical experts, informed the development of the CompAQ-v1. Eleven cognitive interviews were conducted, resulting in the second version of the measure, CompAQ-v2. Psychometric validation resulted in the final 20-item CompAQ measure comprising 5 domains. An abbreviated 5-item measure was also developed (CompAQ-SF).

Conclusion:

CompAQ and CompAQ-SF are instruments intended to evaluate QoL in patients with acne on their face or torso. The former is a 21-item QoL intended for research, while the latter is intended for clinical practice.
Efficient range-free localization using elliptical distance correction in heterogeneous wireless sensor networks
Authors: Wu Wenlan , Wen Xianbin , Xu Haixia , Yuan Liming , Meng Qingxia
Published: January 2018

In this article, a novel range-free localization algorithm is proposed based on the modified expected hop progress for heterogeneous wireless sensor networks where all nodes’ communication ranges are different. First, we construct the new cumulative distribution function expression of expected hop progress to reduce the computational complexity. Then, the elliptical distance correction method is used to improve the accuracy of the estimation distance and simultaneously decrease overhead. Finally, using the modified distance, the coordinate of the unknown node can be obtained by maximum likelihood estimation. Compared with other algorithms for heterogeneous wireless sensor network, the proposed algorithm is superior in the localization accuracy and efficiency when used in random and uniform placement of nodes.
Arthroscopic percutaneous inverted mattress suture fixation of isolated greater tuberosity fracture of humerus
Authors: Jang Suk-Hwan , Song Han-Eui , Choi Seung-Hyuk
Published: January 2018

Objective:

The purpose of the study was to evaluate the clinical and radiographic outcomes of treatment in patients with isolated greater tuberosity (GT) fractures of humerus using arthroscopic percutaneous inverted mattress suture fixation technique.

Materials and methods:

We attempted to use the arthroscopic percutaneous inverted mattress suture fixation technique in 17 consecutive cases with isolated displaced GT fractures. Fourteen patients were successfully treated without switching to other methods and were available for follow-up at a mean of 22 months (range: 17–38 months) after surgery. For assessment of clinical outcomes, we evaluated the range of motion and the visual analog scale (VAS) score, the shoulder index of the American Shoulder and Elbow Surgeons (ASES), and the Korean Shoulder Scale (KSS).

Results:

At the final follow-up, the VAS improved to 1.0 points (range: 0–3), the mean ASES score improved to 86.9 points (range: 78.3–100) and the KSS improved to 88.6 points (range: 82–100) postoperatively. Mean union time was 10 weeks. Mean forward flexion was 167.8° (range: 140–180°), mean external rotation in neutral position was 36° (range: 20–70°), and mean internal rotation was at the 12th thoracic level (range: T6–L3) at final follow-up. Three cases were switched to open surgery after attempted arthroscopic technique due to large fragment or osteoporosis.

Conclusion:

In select cases, the arthroscopic percutaneous inverted mattress suture fixation of GT fracture is a simple and reproducible technique with encouraging early results.
Mindfulness as an Intervention for Recalling Information from a Lecture as a Measure of Academic Performance in Higher Education
Authors: Bennett Rebecca Iranzo , Egan Helen , Cook Amy , Mantzios Michail
Published: January 2018

Higher education students experience heightened levels of stress and anxiety, and report experiencing negative thoughts and emotions, which influence information retention and recall. In a randomized experiment, we assigned participants to either a mindfulness meditation or an audiobook listening condition, and recorded the information recalled from a previously attended lecture, which was controlled for in subsequent analyses for trait resiliency and trait mindfulness. Participants placed in the mindfulness meditation condition recalled significantly more information than participants who were placed in the audiobook listening condition, even when controlling for resiliency and mindfulness. Future directions are suggested in an attempt to expand the literature and research around higher education, mindfulness and individual differences.
Phase 2 trial of a multivalent WT1 peptide vaccine (galinpepimut-S) in acute myeloid leukemia
Authors: Maslak Peter G. , Dao Tao , Bernal Yvette , Chanel Suzanne M. , Zhang Rong , Frattini Mark , Rosenblat Todd , Jurcic Joseph G. , Brentjens Renier J. , Arcila Maria E. , Rampal Raajit , Park Jae H. , Douer Dan , Katz Laura , Sarlis Nicholas , Tallman Martin S. , Scheinberg David A.
Published: January 2018

Key Points

A heteroclitic WT1 peptide vaccine is well tolerated and induces immunologic responses in most acute myeloid leukemia patients post-CR1. Median overall survival for the group of patients vaccinated was not reached but is poised to reach or exceed 67.6 months.
RETRACTED ARTICLE: Integrin-β5, a miR-185-targeted gene, promotes hepatocellular carcinoma tumorigenesis by regulating β-catenin stability
Authors: Lin Zhikun , He Ruiping , Luo Haifeng , Lu Chang , Ning Zhen , Wu Yuanhang , Han Chuanchun , Tan Guang , Wang Zhongyu
Published: January 2018

Background

The tumour microenvironment is essential for cancer progress and metastasis. Integrin-β5 (ITGB5), a member of the integrin family, has been implicated to mediate the interactions of cells with the extracellular matrix (ECM) and promote tumorigenesis in several malignancies. However, the role of ITGB5 in hepatocellular carcinoma (HCC) is still unknown.

Methods

The biological function of ITGB5 in HCC was investigated using migration, colony formation assays. The potential molecular mechanism of ITGB5 in regulating HCC tumorigenesis and β-catenin stabilization was investigated by western blotting, co-immunoprecipitation and ubiquitination assays. The expression level of ITGB5 mediated by miR-185 was confirmed by bioinformatic analysis, luciferase assay. The clinical significance of ITGB5 was based on human tissue microarray (TMA) analysis.

Results

Here, we found that the expression of ITGB5 is increased in HCC tissues. Elevated ITGB5 markedly facilitates HCC cell migration and tumorigenesis in vitro and in vivo. Further mechanistic studies revealed that ITGB5, as a partner of β-catenin, directly interacts with β-catenin and inhibits its degradation, thus leading to WNT/β-catenin activity. Subsequently, we also found that ITGB5 is a direct targeted gene of miR-185. The downregulation of miR-185 in HCC cells promotes an increase in ITGB5. An additional increase of ITGB5 is associated with β-catenin upregulation and a miR-185 decrease in HCC tissues.

Conclusions

Our data reveal that the miR-185-ITGB5-β-catenin pathway plays an important role in HCC tumorigenesis, and ITGB5 may be a promising specific target for HCC therapy.
Pathophysiological and behavioral deficits in developing mice following rotational acceleration-deceleration traumatic brain injury
Authors: Wang Guoxiang , Zhang Yi Ping , Gao Zhongwen , Shields Lisa B. E. , Li Fang , Chu Tianci , Lv Huayi , Moriarty Thomas , Xu Xiao-Ming , Yang Xiaoyu , Shields Christopher B. , Cai Jun
Published: January 2018

Abusive head trauma (AHT) is the leading cause of death from trauma in infants and young children. An AHT animal model was developed on 12-day-old mice subjected to 90° head extension-flexion sagittal shaking repeated 30, 60, 80 and 100 times. The mortality and time until return of consciousness were dependent on the number of repeats and severity of the injury. Following 60 episodes of repeated head shakings, the pups demonstrated apnea and/or bradycardia immediately after injury. Acute oxygen desaturation was observed by pulse oximetry during respiratory and cardiac suppression. The cerebral blood perfusion was assessed by laser speckle contrast analysis (LASCA) using a PeriCam PSI system. There was a severe reduction in cerebral blood perfusion immediately after the trauma that did not significantly improve within 24 h. The injured mice began to experience reversible sensorimotor function at 9 days postinjury (dpi), which had completely recovered at 28 dpi. However, cognitive deficits and anxiety-like behavior remained. Subdural/subarachnoid hemorrhage, damage to the brain-blood barrier and parenchymal edema were found in all pups subjected to 60 insults. Proinflammatory response and reactive gliosis were upregulated at 3 dpi. Degenerated neurons were found in the cerebral cortex and olfactory tubercles at 30 dpi. This mouse model of repetitive brain injury by rotational head acceleration-deceleration partially mimics the major pathophysiological and behavioral events that occur in children with AHT. The resultant hypoxia/ischemia suggests a potential mechanism underlying the secondary rotational acceleration-deceleration-induced brain injury in developing mice.
The central role of DNA damage and repair in CAG repeat diseases
Authors: Massey Thomas H. , Jones Lesley
Published: January 2018

Diseases such as Huntington's disease and certain spinocerebellar ataxias are caused by the expansion of genomic cytosine-adenine-guanine (CAG) trinucleotide repeats beyond a specific threshold. These diseases are all characterised by neurological symptoms and central neurodegeneration, but our understanding of how expanded repeats drive neuronal loss is incomplete. Recent human genetic evidence implicates DNA repair pathways, especially mismatch repair, in modifying the onset and progression of CAG repeat diseases. Repair pathways might operate directly on repeat sequences by licensing or inhibiting repeat expansion in neurons. Alternatively, or in addition, because many of the genes containing pathogenic CAG repeats encode proteins that themselves have roles in the DNA damage response, it is possible that repeat expansions impair specific DNA repair pathways. DNA damage could then accrue in neurons, leading to further expansion at repeat loci, thus setting up a vicious cycle of pathology. In this review, we consider DNA damage and repair pathways in postmitotic neurons in the context of disease-causing CAG repeats. Investigating and understanding these pathways, which are clearly relevant in promoting and ameliorating disease in humans, is a research priority, as they are known to modify disease and therefore constitute prevalidated drug targets.
Should We Reject the Natural Rate Hypothesis?
Authors: Blanchard Olivier
Published: January 2018

Fifty years ago, Milton Friedman articulated the natural rate hypothesis. It was composed of two sub-hypotheses: First, the natural rate of unemployment is independent of monetary policy. Second, there is no long-run trade-off between the deviation of unemployment from the natural rate and inflation. Both propositions have been challenged. The paper reviews the arguments and the macro and micro evidence against each. It concludes that, in each case, the evidence is suggestive, but not conclusive. Policymakers should keep the natural rate hypothesis as their null hypothesis, but keep an open mind and put some weight on the alternatives.
Com as próprias pernas
Authors: Sheng Hsia Hua , Carrera Junior José Marcos
Published: January 2018

Multinacionais brasileiras devem diminuir sua dependência de recursos estatais e definir melhor sua estratégia global para os próximos anos.
Um padrão inovador
Authors: Santos Edilene Santana
Published: January 2018

A convergência para um modelo internacional único de contabilidade vem transformando, para melhor, as informações financeiras das empresas brasileiras.
O líder financeiro sob pressão
Authors: Almeida Antonio José Rocha de
Published: January 2018

Com a inovação digital, a proliferação de dados, um ambiente de maior risco e stakeholders mais exigentes, os chief financial officers (CFOs) precisam reorganizar suas funções.
Compensa investir em inovação?
Authors: Furtado Cláudio Vilar , Belluzzo Giovane Spagnuolo
Published: January 2018

À crescente indústria de fundos de private equity e venture capital cabe questionar se os retornos econômicos serão compatíveis com os riscos assumidos.
A importância da gestão da cadeia de suprimentos
Authors: Miguel Priscila Laczynski de Souza
Published: January 2018

Agregar valor à entrega permite às empresas se diferenciarem
Tendências na gestão da saúde
Authors: Vecina Neto Gonzalo , Malik Ana Maria
Published: January 2018

Como pagar a conta do sistema e como garantir o acesso de todos aos novos tratamentos são as duas questões deste século. A resposta está nas possíveis melhorias com a revolução tecnológica.
Nova administração nos hospitais públicos
Authors: Rehem Renilson
Published: January 2018

Diversas organizações sociais vêm assumindo, com excelentes resultados, serviços de estados e municípios. É o melhor modelo nos dias de hoje, mas não há solução mágica.
Na visão dos especialistas
Authors: GV-executivo Equipe da redação -
Published: January 2018

O que faz e o que precisa saber um administrador na área de saúde?
É difícil abrir mão de bens materiais
Authors: Dana Samy
Published: January 2018

As pessoas têm aversão a perder o que possuem. Para evitar o excesso de apego, uma boa saída é criar o hábito de negociar.
Assédio moral nas organizações
Authors: Barros Amon
Published: January 2018

No contexto de crise atual, de alta carga de estresse, cabe mais atenção para evitar que comportamentos abusivos se tornem a norma.
Entre o desagradável e o desastroso
Authors: Sandroni Paulo
Published: January 2018

A política econômica do governo temer penaliza a maioria da população e livra o máximo possível o empresariado.
A nova fonte de valor econômico
Authors: Vendramini Annelise , Belinky Aron
Published: January 2018

Uma gestão que incorpore aspectos socioambientais será cada vez mais fundamental para criar e proteger o capital das empresas.
Risco ou oportunidade?
Authors: Osório Guarany , Santos Inaiê Takaes
Published: January 2018

Instrumentos para precificar emissões de gases de efeito estufa avançam no Brasil e no mundo. Falta usar o potencial nacional de produção industrial de baixa intensidade carbônica como diferencial competitivo.
O papel do consumo
Authors: Betiol Luciana Stocco
Published: January 2018

Para o real desenvolvimento sustentável, as soluções pelo lado da oferta de mercado não serão suficientes, principalmente em um cenário em que a população mundial já consome mais de um quarto acima do que o planeta pode repor naturalmente.
Gestão de carreira em um mundo de talentos líquidos
Authors: Nóbrega Marcelo de Freitas
Published: January 2018

Numa conjuntura volátil, com vínculos de trabalho superficiais, a insegurança tornou-se parte da vida. Como agir, se fomos educados para gerenciar nossas carreiras de forma linear e progressiva?
Economia e política descolaram-se?
Authors: Sandroni Paulo
Published: January 2018

Trocados entre o loiro desvairado e o gordinho sinistro poderão resultar em um impensável conflito nuclear, empurrando o mundo para um precipício incompatível com um bom clima para os negócios?
Mulher de fibra
Authors: Wilner Adriana
Published: January 2018

No passado, que as áreas operacionais eram consideradas lugares dos machões, dos fortões, da peãozada. Questionavam: “O que mulher tem a ver com esse universo?”
Desafios da gestão de riscos
Authors: Pereira Susana Carla Farias , Pereira Luís Henrique
Published: January 2018

As cadeias de suprimentos com conexões ao redor do mundo podem trazer vantagens competitivas, mas exigem atenção redobrada dos gestores, pois são mais sujeitas a rupturas do que as que operam localmente
Serviço completo
Authors: Tescari Fábio Campos , Scalese Junior Eduardo Antonio
Published: January 2018

O customer service, que antes só cuidava das reclamações dos clientes, agora enfrenta o desafio de conectar a área comercial à cadeia produtiva e, assim, proporcionar atendimento flexível e integral.
Inteligência em compras
Authors: Biazzin Cristiane
Published: January 2018

Dados e processos são cada vez mais transparentes e acessíveis. O que falta é desenvolver competências para analisar tantas informações e usá-las adequadamente na tomada de decisões.
Movimentações das cadeias globais
Authors: Finger Andrew Beheregarai
Published: January 2018

Nas Global Value Chains (GVCs), a disputa é por quem domina o poder e consegue conquistar maior valor agregado.
Metabolie epidermal necrosis (hepatocutaneous syndrome) in the dog: A clinical and pathological review of 6 spontaneous cases
Authors: KOUTINAS (X.K. ΚΟΥΤΙΝΑΣ) C. K. , KOUTINAS (Α.Φ. ΚΟΥΤΙΝΑΣ) A. F. , SARIDOMICHELAKIS (Μ.Ν. ΣΑΡΙΔΟΜΙΧΕΛΑΚΗΣ) M. N. , KALDRYMIDOU (Ε. ΚΑΛΔΡΥΜΙΔΟΥ) H. , ROUBIES (Ν. ΡΟΥΜΠΙΕΣ) N.
Published: January 2018

Metabolic epidermal necrosis was diagnosed in 6 dogs admitted to the Clinic of Companion Animal Medicine, Faculty of Veterinary Medicine, A.U.T., between 1989 and 1998. Four of these animals were males and 2 females with an age range of 8 to 11.5 years. Bilaterally symmetrical (5/6) or asymmetrical (1/6) skin lesions characterized by alopecia – hypotrichosis (5/6), erythema (6/6), depigmentation (3/6), epidermal colarettes (3/6), ulcers and erosions (6/6), crusts (6/6), footpad and nose hyperkeratosis (5/6), edema (4/6), exudation (3/6), pustules (2/6), scales (2/6) and papules (1/6) were observed in all of the dogs. These lesions were located on the limbs (6/6), the external genitalia (5/6), ventral abdomen (4/6), mucocutaneos junctions (4/6), pressure points (3/6), distal extremities (3/6), nasal philthrum (3/6), muzzle (2/6), axillae (1/6) and on the dorsal aspect of the body trunk (1/6). The most important clinicopathologic findings included anemia (5/6), leucocytosis (3/6), thrombocytopenia (1/6), hypoalbuminaemia (4/5), hyperglycemia (3/6), increased alkaline phosphatase (4/6) and alanino-aminotransferase (5/6) activities, hypocalcaemia (2/5), proteinuria (1/6) and glycosuria (3/6). Liver histopathology, carried out in 4 dogs, revealed vacuolar hepatopathy in all of them. The same underlying disease was suspected in one additional case, whereas pancreatic glucagonoma was a possibility for the remaining dog. Systemic an/or topical treatment, that was attempted in 3 dogs, was unrewarding. All the 6 dogs died (3/6) or were euthanized (3/6) 2 to 17 months after the appearance of the skin lesions.
Brachial plexus neuropathy or neuritis in the dog and cat: A report of 4 spontaneous cases
Authors: KOUTINAS (Α.Φ. ΚΟΥΤΙΝΑΣ) A. F. , POLIZOPOULOU (Ζ.Σ. .ΠΟΛΥΖΟΠΟΥΛΟΥ) Z. S. , GEORGIADIS (Γ. ΓΕΩΡΓΙΑΔΗΣ) G. , SARIDOMICHELAKIS (Μ.Ν. ΣΑΡΙΔΟΜΙΧΕΛΑΚΗΣ) M. N. , PATSIKAS (Μ.Ν. ΠΑΤΣΙΚΑΣ) M.
Published: January 2018

One kitten and three adult dogs were admitted with a history of an acute or progressive lower motor neuron disease involving asymmetrically the thoracic limbs. Neurogenic muscular atrophy was also present affecting the relevant muscles in all the animals. As no other neuromuscular abnormalities could be detected on physical, radiological and clinicopathologic examination, a tentative diagnosis of brachial plexus neuropathy or neuritis was made. In the cat diagnosis was confirmed by electrophysiological testing demonstrating decreased sensory conduction velocity of the ulnar nerve and denervation potentials. The administration of prednisolone at antiinflammatory dosage for 3 weeks abolished or decreased the severity of the clinical signs in all the cases. Recurrence of the disease, one to 6 months after the cessation of treatment, was detected or reported for the 3 dogs where a follow-up was possible.
Investigations on the liver function and its prognostic value in dairy cows with hypocalcaemic paralysis
Authors: PANOUSIS (Ν. ΠΑΝΟΥΣΗΣ) N. , ROUBLES (Ν. ΡΟΥΜΠΙΕΣ) N. , KARATZIAS (Χ. ΚΑΡΑΤΖΙΑΣ) H.
Published: January 2018

Investigations on the liver function and its prognostic value were performed in dairy cows with hypocalcaemic paralysis. Among the 107 animals affected by milk fever 22 showed signs of liver insufficiency. In spite of the therapy indicated, 7 of these animals were not cured. The remaining 85 sick cows, which had showed a physiologic liver function, were cured after the first therapy was applied.
Epizootiologic survey of selenium and vitamin E concentrations in dairy cows of Thessaioniki’s county
Authors: CHRISTODOULOPOULOS (Γ. ΧΡΙΣΤΟΔΟΥΛΟΠΟΥΛΟΣ) G. , ROUBLES (Ν. ΡΟΥΜΠΙΕΣ) N. , KARATZIAS (Χ. ΚΑΡΑΤΖΙΑΣ) H. , PAPASTERIADIS (Α. ΠΑΠΑΣΤΕΡΙΑΔΗΣ) A.
Published: January 2018

The purpose of this research was the study of selenium (Se) and vitamin E (vit. E) concentrations in dairy cows blood within the region of Thessaloniki. The survey included 65 dairy cow farms in the region of Thessaloniki from which samples of blood were collected from 10 female adult animals from every farm. Out of the total 650 cows examined in the 65 farms, 40% presented deficient concentration of Se in blood (<0,08 μg/ml), 29% marginally deficient concentration (0,08-0,12 μg/ml) and only 31% normal concentration (>0,12 μg/ml). Regarding vit. E, only 4%, out of the 650 cows examined, had deficient concentration in the blood plasma (<2 μg/ml). It was concluded from the above that, in the region of Thessaloniki, a significant percentage of the dairy cows run the risk of Se deficiency diseases. On the contrary, the case of vit. E deficiency, should be regarded as improbable for these animals.
Nitrate and nitrite residues in Greek pastirma
Authors: TYRPENOU A. E. , GOUTA (E.H. ΓΟΥΤΑ) E. H. , TSIGOURI (Α.Δ. ΤΣΙΓΟΥΡΗ) A. D. , VLASIOTIS (Χ.Ν. ΒΛΑΣΙΩΤΗΣ) Ch. N.
Published: January 2018

In this research, for the investigation of the nitrate and nitrite content of Greek pastirma samples coming from several Greek meat processing factories were examined. Nitrate and nitrite determination was carried out following the standard methods of the International Standard Organization ISO No. 2918/751 and 3091/75.2 According to these Standard Operating Procedures-SOPs, reducing capacity of cadmium column should always has to be >90%. Because this critical point usually causes problems [never was >70%], we succeeded and validated this point by amending 3 points of the Standard Operating Procedure [SOP]. These amendments referred to the replacement of the buffer pH 9,6 of the cadmium column with the buffer pH 12,0 according to the method of Margeson et al., 1980.3 The result was that the reducing capacity of the cadmium column increased from 70% to 90-105%. Thirty pastirma samples have been tasted and the concentrations determined ranged from 0,85 to 189,65mg/kg for nitrites and from 2,66 to 639,67mg /kg for nitrates expressed as sodium nitrite. Nitrites, in 56,66% of the samples and nitrates in 83,33% of the samples were ranging in the limits foreseen by the Greek Legislation.
Meningoencephalitis caused by thermal disbudding in goat kids
Authors: XYLOURI (Ε. ΞΥΛΟΥΡΗ) E. , SABATAKOU (Ο. ΣΑΜΠΑΤΑΚΟΥ) O. , SAKELLARIADI (SAKELLARIADI Th.) Th.
Published: January 2018

A case of kids' meningoencephalitis due to head heat application during a thermal disbudding procedure is described. The case was observed in an organic goat farming where the kids about the 7-8th day after their birth were exposed to the application of excessive heat (with an electric device) during disbudding. 2,2% of the kids presented neurological signs and died in few days. The post mortem examination confirmed that the cause was destruction of the frontal bone, at the point where the thermal electric procedure was applied, leading to irreparable injury.
Current aspects on vaccines and immunization in the dog and cat. Part I. General aspects-Vaccines and vaccinations in the dog
Authors: MYLONAKIS (Μ.Μ. ΜΥΛΩΝΑΚΗΣ) M. M. , KOUTINAS (Α.Φ. ΚΟΥΤΙΝΑΣ) A. F. , PLEVRAKI (Κ.Γ. ΠΛΕΥΡΑΚΗ) K. G.
Published: January 2018

Immunoprophylaxis plays a pivotal role within the frame of contemporary preventive medicine, enabling the practicing veterinarian to control the most common infectious diseases that may pose a threat on the health status or life itself in the dog and cat. Passive immunization is the administration of preformed antibodies in animals of the same or different species. An alternative to maternal immunity, the natural way of passive immunity, is the use of hyperimmune serum or plasma in colostrum-deprived neonates, in unvaccinated puppies and kittens that belong to high risk groups and in immunocompromised animals. In small animal practice the main vaccine types currently in use for vaccination, an active immunization procedure, include the modified live vaccines, the inactivated and the subunit vaccines. The sound knowledge of these vaccines special features and the potential causes of vaccination failures are essential if a successful vaccination schedule is to be implemented. Vaccinations can start at the 6th week of life in colostrum-afforded neonates while in colostrum-deprived puppies at the 4th week with the use of inactivated vaccines. In the dog, infectious diseases such as distemper, infectious hepatitis, leptospirosis (L. canicola, L. icterohaemorrhagiae), rabies, infectious tracheobronchitis, parvovirus infection, Coronavirus enteritis and babesiosis can be prevented by the use of vaccines which are currently available.
Gastrointestinal nematodes of equine in the region of Thessaloniki
Authors: THEODORIDIS (Ι. ΘΕΟΔΩΡΙΔΗΣ) Y. , FOUNTA (Α. ΦΟΥΝΤΑ) A. , GEORGOULAKIS (Ι. ΓΕΩΡΓΟΥΛΑΚΗΣ) I.
Published: January 2018

During a period of approximately two months (January-February 1995), 91 out of 119 riding horses of the seven riding clubs of Thessaloniki their feces were examined. The purpose of this survey was to find out and identify parasites after examining their reproductive elements. In the feces of the horses of two riding clubs had not been found any reproductive elements, while in the remaining five were found strongylid eggs in population whieh ranged from 2-5 to 85-94 / g of feces; per club ( rate infection 68; 1 %). After the examination of the 3rd stage larval, which obtained from fecal culture, were identified, from large strongylids (sub-family Strongylinae), the species Triontophorus sp (42%), Strongylus equinus (29%), Strongylus edentatus (21 %), Strongylus vulgaris (6%), and Trichostrongylus axei (2%) from the family of Trichostrongylidae. From the small strongylids (sub-family Cyathostominae) were identified the genera Cyathostomum (42%), Cylicostephanus (24%), Gyalocephalus (22%) and Poteriostomum (12%). The identified genera Triontophorus, Gyalocephalus and Poteriostomum as well as the species Trichostrongylus axei, are mentioned for the first time in horses in greek region.
Alternative methods of replacement of laboratory animals in biomedical research
Authors: KOSTOMITSOPOULOS (Ν.Γ. ΚΩΣΤΟΜΗΤΣΟΠΟΥΛΟΣ) N. G.
Published: January 2018

Society's sensitivity on the use of laboratory animals, the need for the improvement of the scientific value of the results and the high costs urged the biomedical community to look for alternative methodologies which could replace or reduce the number of animals being used or refine the existing experimental techniques. Replacement alternatives can be classified, according to the applied methology, in in vitro and immunological techniques and in methods which use «lower» organisms or vertebrates at early developmental stages, mathematical or computer models, human studies or data banks. Using replacement alternatives may raise a lot of problems such as the quality of results. Validation of these methods is a complicated and time consuming process with high but fair hurdles which aim at human safety.
Termination of pregnancy in the bitch
Authors: DELLIS (Σ. ΝΤΕΛΛΗΣ) S. C. , BOSCOS (Κ. Μ. ΜΠΟΣΚΟΣ) C. M.
Published: January 2018

Unwanted mating is a relatively common occurrence in bitches, often as a result of ignorance or carelessness of owners. Once it has been established that mating has occurred during the fertile stage of the oestrous cycle, there are various options for the prevention of pregnancy. In bitches not intended for future breeding, ovariohysterectomy is the best option. In breeding or potentially breeding bitches a number of treatments have been investigated, but most of them were associated with side effects and complications. This paper focus on currently available methods to prevent or terminate unwanted pregnancy in bitches and emphasizes on dosage, time of intervention, duration, efficacy, and adverse effects of these treatments, as well as on products that could be used and are available in Greece.
Acid-base status and electrolyte concentrations in 32 dogs with parvoviral enteritis
Authors: KOUTINAS (Α.Φ. ΚΟΥΤΙΝΑΣ) A. F. , SARIDOMICHELAKIS (Μ.Ν. ΣΑΡΙΔΟΜΙΧΕΛΑΚΗΣ) M. N. , RALLIS (Τ. ΡΑΛΛΗΣ) T. , KRITSEPI (Μ. ΚΡΙΤΣΕΠΗ) M. , ADAMAMA-MORAITOU (Κ. ΑΔΑΜΑΜΑ-ΜΩΡΑΪΤΟΥ) K.
Published: January 2018

A total of 32 dogs with clinical signs compatible with parvoviral enteritis were collected and blood pH, 02 and C02 partial pressure, bicarbonate and hemoglobin concentrations and hemoglobin saturation were measured. Sodium and potassium concentrations were also determined by photometry in the serum of venous blood samples. Acidemia, due to metabolic acidosis, was the most common abnormality, found in 17/32 animals. Alkalemia, attributed to respiratory alkalosis, was seen only in 3 dogs. Nine animals with atrerial blood pH found to be within normal limits had low bicarbonate concetration and reduced C02 partial pressure. This discrepancy could be attributed to the development of compensatory mechanisms or a mixed acid-base disorder. Hypokalemia (2/32) or hyperkalemia (2/32) were not a common finding, compared to hyponatremia (5/32) and hypernatremia (12/32).
Chlamydial infections in swine industry and their significance
Authors: KRITAS (Σ.Κ. ΚΡΗΤΑΣ) S. K. , SAOULIDIS (Κ. ΣΑΟΥΛΙΔΗΣ) K. , TSINAS (Α. ΤΣΙΝΑΣ) A. , PAPADOPOULOS (Ο. ΠΑΠΑΔΟΠΟΥΛΟΣ) O. , KYRIAKIS (Σ.K. ΚΥΡΙΑΚΗΣ) S. K.
Published: January 2018

Chlamydial infections in animals, particularly those in ruminants and poultry, have been well studied for the last decades. However, no much information exist on such infections in pigs. This paper reviews the recent knowledge in various countries as well as in Greece on the role of the microorganism on causing disease in swine with emphasis on pathogenesis, epizootiology and diagnosis. Particular attention is given on the newly-described chlamydial strains as well as the recent experimental information and views which implicate the microbe as pathogen. Finally, some measures with respect the prevention and control against Chlamydiae are suggested for the Greek pig industry.
Effect of Buserelin on corpus luteum activity and the fertility of dairy cows
Authors: SARATSIS (Φ. ΣΑΡΑΤΣΗΣ) Ph. , SCHMIDT-ADAMOPOULOU (Β. SCHMIDT-ΑΔΑΜΟΠΟΥΛΟΥ) B. , YPSILANTIS (Π. ΥΨΗΛΑΝΤΗΣ) P. , BROZOS (Χ. ΜΠΡΟΖΟΣ) Ch. , DEMERTZIS (Α. ΔΕΜΕΡΤΖΗΣ) A.
Published: January 2018

112 selected Friesian dairy cows that had calved at least once, were used. The animals were divided into two groups, A (n = 55) and Β (n=57). On the 12th day post insemination (p.i.) 12 μg buserelin (3ml Receptal - Hoechst) and 3 ml normal saline (placebo) were injected intramuscularly to the animals of groups A and Β (controls), respectively. Milk progesterone concentration was measured in even animal from the day of insemination until the 24th day p.i.. A significant increase (P<0.05) in pregnancy rate (19.7%) was observed in the animals of group A. Also, the concentration of progesterone in the pregnant animals (14th - 24th day p.i.) as well as in the non pregnant (14th - 16th day p.i.) ones in group A was significantly increased (P<0.05) compared to that of the controls'. The duration of the estrous cycle of the non pregnant animals did not differ significantly (P>0.05) between the two groups. It is concluded that buserelin administration on the 12th day p.i. improved the fertility of dairy cows significantly by means of an antiluteolytic effect, through an increase in progesterone concentration, during the crucial period of maternal recognition of pregnancy.