bluuebunny/crossref_metadata_2025 huggingface dataset:
Performance evaluation of the cobas SARS-CoV-2 Duo, a novel qualitative and quantitative assay, for the detection of SARS-CoV-2 RNA
Authors: Su Yang-Di , Lai Chih-Cheng , Lin Tsai-Hsiu , Chen Wei-Cheng , Hsueh Po-Ren
Published: November 2023

Viral load quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a critical measure for monitoring clinical responses of patients with COVID-19. The cobas SARS-CoV-2 Duo (Roche Molecular Systems, Inc.) uses the cobas 6800/8800 platform and is an automated real-time reverse transcription PCR (RT-PCR) assay for the qualitative detection of SARS-CoV-2 RNA (ORF1a and ORF1a/b non-structural regions) in nasal and nasopharyngeal specimens. This assay also performs quantitation of the SARS-CoV-2 RNA level in the collected specimen. The precision, accuracy, and linearity of the cobas SARS-CoV-2 Duo test were assessed and correlated with the cycle threshold (Ct) value of the cobas SARS-CoV-2 assay, and the cobas Liat SARS-CoV-2 and influenza A/B assay (Inf A/B) (cobas Liat) in 201 clinical nasopharyngeal swab specimens. The analytical measurement range of the cobas SARS-CoV-2 Duo has been verified to be 1.0E + 02 IU/mL to 1.0E + 09 IU/mL. The between- and within-run precision evaluation and the linearity results (
R
2
= 0.9961) of the cobas SARS-CoV-2 Duo were acceptable based on the verification criteria. A total of 201 nasopharyngeal specimens were evaluated. The positive, negative, and total agreements between the cobas SARS-CoV-2 Duo and the cobas SARS-CoV-2 assay were 93.5% (157 of 168), 75% (33 of 44), and 92.5% (186 of 201), respectively. The qualitative results (Ct values) from the cobas SARS-CoV-2 and the cobas Liat demonstrated good agreement with the quantitative results from the cobas SARS-CoV-2 Duo assay. In conclusion, the diagnostic performance of the cobas SARS-CoV-2 Duo assay is appropriate. It is a precise, accurate, and sensitive method for the detection of SARS-CoV-2 RNA and is comparable to two qualitative assays.

IMPORTANCE

Quantitative SARS-CoV-2 tests for viral load are necessary to guide patient treatment, as well as to determine infection control measures and policies. Although the real-time RT-PCR assays can report the Ct value to estimate the viral load, there are several serious concerns regarding the use of Ct values. Importantly, Ct values can vary significantly among between- and within-run methods. The diagnostic performance of the cobas SARS-CoV-2 Duo is appropriate. It is a precise, accurate, and sensitive method for the detection of SARS-CoV-2 RNA and is comparable to two qualitative assays (the cobas SARS-CoV-2 and the Liat cobas SARS-CoV-2 and Inf A/B). In contrast, using the Ct value to estimate viral load is not reliable, and utilization of a quantitative detection test, such as the cobas SARS-CoV-2 Duo, to accurately measure the viral load is needed.
Treatments for partial remission of major depressive disorder: a systematic review and meta-analysis
Authors: Gülpen Joost , Brouwer Marlies E , Geurtsen Gert J , van Dis Eva A M , Denys Damiaan A J P , Bockting Claudi L
Published: November 2023

Question

Partial remission of major depressive disorder (MDD) is a debilitating and distressing clinical state related to chronicity, morbidity and relapse. Although one-third of patients remit partially, evidence for treatment efficacy is unclear. We provide an overview of treatment options and their efficacy.

Study selection and analysis

Embase, PsycINFO, Medline and SCOPUS were systematically searched through February 2023. Included were randomised controlled trials (RCTs) examining any treatment in patients with partially remitted MDD aged 13–65 years, reporting data on severity, remission or relapse.

Findings

Seven RCTs examining psychotherapy including 1024 patients were eligible. There were not enough RCTs to examine effects of pharmacotherapy. Psychotherapy was associated with lower depressive symptom severity at post-treatment (Hedges’ g=0.50; 95% CI 0.23 to 0.76), but not at follow-up up to 1 year (Hedges’ g=0.36; 95% CI −0.30 to 1.02) or longer (Hedges’ g=0.02; 95% CI −0.09 to 0.12). Psychotherapy was associated with superior remission rates at post-treatment (OR 2.57; 95% CI 1.71 to 3.87) and follow-up 6 months or longer (OR 1.75; 95% CI 1.21 to 2.53), although not with improved relapse rates at post-treatment (OR 0.17; 95% CI 0.01 to 4.83) or follow-up 6 months or longer (OR 0.46; 95% CI 0.21 to 1.03). Overall methodological quality was poor.

Conclusions

Psychotherapy targeting partial remission may be effective in lowering depressive symptom severity and patients may potentially achieve full remission twice as likely. Yet, long-term and prophylactic effects are lacking. Given the risk of chronicity, more high-quality RCTs are needed.

PROSPERO registration number

CRD42020188451.
Test the weak cosmic supervision conjecture in dark matter-black hole system
Authors: Meng Liping , Xu Zhaoyi , Tang Meirong
Published: November 2023

There is a possibility that the event horizon of a Kerr-like black hole with perfect fluid dark matter (DM) can be destroyed, providing a potential opportunity for understanding the weak cosmic censorship conjecture of black holes. In this study, we analyze the influence of the strength parameter of perfect fluid DM on the destruction of the event horizon of a Kerr-like black hole with spinning after injecting a test particle and a scalar field. We find that, when a test particle is incident on the black hole, the event horizon is destroyed by perfect fluid dark matter for extremal black holes. For nearly extremal black holes, when the dark matter parameter satisfies $$\alpha \in \left( -r_{h} , 0\right) \cup \left( r_{h} ,k_2\right) $$

α
∈

-

r
h

,
0

∪

r
h

,

k
2

i.e. $$(A<0),$$

(
A
<
0
)
,

the event horizon of the black hole will not be destroyed; when the dark matter parameter satisfies $$\alpha \in \left( k_1 ,-r_{h} \right] \cup \left[ 0,r_{h}\right] $$

α
∈

k
1

,
-

r
h

∪

0
,

r
h

i.e. $$(A\ge 0),$$

(
A
≥
0
)
,

the event horizon of the black hole will be destroyed. When a classical scalar field is incident into the black hole in the extremal black hole case, we find that the range of mode patterns of the scalar field that can disrupt the black hole event horizon is different for different values of the perfect fluid dark matter strength parameter. In the nearly extremal black hole case, through our analysis, we have found when $$\alpha \ne 0 $$

α
≠
0

and $$\alpha \ne \pm \ r_h$$

α
≠
±

r
h

i.e. $$A\ne 0,$$

A
≠
0
,

the event horizon of the black hole can be disrupted. Our research results indicate that dark matter might be capable of breaking the black hole horizon, thus potentially violating the weak cosmic censorship conjecture.
Lorentzian manifolds: A characterization with a type of semi-symmetric non-metric connection
Authors: Suh Young Jin , Chaubey Sudhakar Kumar , Khan Mohammad Nazrul Islam
Published: November 2023

This paper characterizes the Lorentzian manifolds endowed with a semi-symmetric non-metric [Formula: see text]-connection (briefly, ssnmρc). First, the existence of semi-symmetric non-metric connection (ssnmc) on Lorentzian manifold is established, and it is shown that an [Formula: see text]-dimensional Lorentzian manifold equipped with an ssnmρc is a generalized Robertson–Walker spacetime. We also establish the condition for a Lorentzian manifold together with an ssnmρc to be a Robertson–Walker spacetime. In this series, the properties of Ricci semi-symmetric Lorentzian manifold endowed with an ssnmρc, almost Ricci solitons and gradient Ricci solitons are explored. Finally, a non-trivial example of Lorentzian manifold admits an ssnmρc, which is constructed to verify some of our results.
On topology changes in quantum field theory and quantum gravity
Authors: Schulz Benjamin
Published: November 2023

Two singularity theorems can be proven if one attempts to let a Lorentzian cobordism interpolate between two topologically distinct manifolds. On the other hand, Cartier and DeWitt-Morette have given a rigorous definition for quantum field theories (QFTs) by means of path integrals. This paper uses their results to study whether QFTs can be made compatible with topology changes. We show that path integrals over metrics need a finite norm for the latter and for degenerate metrics, this problem can sometimes be resolved with tetrads. We prove that already in the neighborhood of some cuspidal singularities, difficulties can arise to define certain QFTs. On the other hand, we show that simple QFTs can be defined around conical singularities that result from a topology change in a simple setup. We argue that the ground state of many theories of quantum gravity will imply a small cosmological constant and, during the expansion of the universe, will cause frequent topology changes. Unfortunately, it is difficult to describe the transition amplitudes consistently due to the aforementioned problems. We argue that one needs to describe QFTs by stochastic differential equations, and in the case of gravity, by Regge calculus in order to resolve this problem.
Large Fluctuations in Amplifying Graphs
Authors: Lepri Stefano
Published: November 2023

In this paper, we consider a model for chaotic diffusion with amplification on graphs associated with piecewise-linear maps of the interval [S. Lepri, Chaotic fluctuations in graphs with amplification, Chaos, Solitons & Fractals 139 (2020) 110003]. We determine the conditions for having fat-tailed invariant measures by considering approximate solution of the Perron–Frobenius equation for generic graphs. An analogy with the statistical mechanics of a directed polymer is presented that allows for a physically appealing interpretation of the statistical regimes. The connection between non-Gaussian statistics and the generalized Lyapunov exponents [Formula: see text] is illustrated. Finally, some results concerning large graphs are reported.
Dynamic Response of a Rotating Beam with Elastic Restraints in Forward Flight
Authors: Wang Feiyu , Su Zhu , Wang Lifeng
Published: November 2023

This paper presents the dynamic response of a rotating beam with elastic restraints in forward flight. The motion equations of the system are established by Hamilton’s principle. The structure model is established by Euler-Bernoulli beam theory. The influence of elastic restraints and centrifugal force is considered in the form of potential energy. The aerodynamic model is established by Greenberg theory and considered in the form of an external force. A modified Fourier series method is used to expand the displacement field. The stiffness intervals of boundary springs corresponding to the elastic restraints are determined by natural frequencies. The effects of advance ratios on the dynamic response of the system are studied. Then, the effects of spring stiffness in different directions are compared. The results show that the displacements and velocities of the rotating beam increase with the advance ratio. The displacement amplitudes of the rotating beam increase as the stiffness of boundary springs decrease.
Strategy Requires Execution
Authors: Kolko Jon
Published: November 2023

The Details Aren't Boring. The Details Are the Way to Success.
Editors’ Choice—Structural, Electrical, and Luminescent Properties of Orthorhombic κ-Ga2O3 Grown by Epitaxial Lateral Overgrowth
Authors: Nikolaev V. I. , Polyakov A. Y. , Myasoedov A. V. , Pavlov I. S. , Morozov A. V. , Pechnikov A. I. , Lee In-Hwan , Yakimov E. B. , Vasilev A. A. , Scheglov M. P. , Kochkova A. I. , Pearton S. J.
Published: November 2023

The properties of orthorhombic κ-Ga2O3 films grown by Epitaxial Lateral Overgrowth (ELOG) were studied by Scanning Transmission Electron Microscopy (STEM), X-ray diffraction, capacitance-voltage profiling, Microcathodoluminescence (MCL) spectroscopy and imaging. ELOG mask was formed by deposition of SiO2 stripes on TiO2 buffer prepared on basal plane sapphire, with the stripes going along the [11

2

̅

0] direction of sapphire. κ-Ga2O3 ELOG growth was performed using Halide Vapor Phase Epitaxy (HVPE), with ELOG wing of the structure formed by lateral overgrowth over the 20 μm-wide SiO2 stripes, while growth in between the stripes proceeded initially by vertical growth in the 5-μm-wide windows. TEM analysis showed that the material in the windows comprised 120o rotational nanodomains typical of κ-Ga2O3, while, in the wing regions, the material was single-domain monocrystalline. The films were conducting, with the net donor density close to 1013 cm−3. The data suggested the material in the windows have much higher resistance than in the wings. MCL spectra and imaging revealed much higher density of nonradiative recombination centers in the windows than in the wings.
Enhancing Nurses’ Well-Being: Exploring the Relationship between Transformational Leadership, Organizational Justice, and Quality of Nursing Work Life
Authors: Abdullatif Ibrahim Ibrahim , Hashem El-Monshed Ahmed , Gamal El-Sehrawy Mohamed , Elamir Hossam , Mohamed Abdelrahim Samah
Published: November 2023

Aim. The study’s aim was to examine the relationship between transformational leadership, organizational justice, and nurses’ quality of work life. Background. There is an increasing emphasis on the welfare of nurses and their influence on healthcare services. There is a dearth of comprehensive studies on the influence of transformational leadership and justice within organizations on work-related quality of life. Understanding these connections is critical for improving workplace dynamics and fostering nurse well-being. Methods. This cross-sectional research included a convenience sample of nurses working in a general hospital. The data were obtained through self-report questionnaires encompassing three reliable and validated scales: the global transformational leadership scale, organizational justice scale, and quality of nursing work-life scale. The data were then analyzed with SPSS 23 and statistical methods (descriptive statistics, Pearson correlation coefficient, and hierarchical linear regression). This study adhered to the STROBE guidelines. Results. A sum of 527 out of 566 nurses responded to the questionnaires (response rate = 93.1%). The nurses had a high perception of transformational leadership behaviors but had a more moderate view of organizational fairness and the quality of their nursing work life. Transformational leadership was significantly associated with organizational fairness. Also, a positive relationship was found between transformational leadership, justice within organizations, and nurses’ quality of work life. Conclusions. Nurses who consider their leaders as transformative and have the sense of a fair organizational climate are more likely to have higher levels of quality of work life. Implications for Nursing Management. Nurse managers should strive to develop transformational leadership behaviors and promote organizational justice to enhance the well-being and satisfaction of nurses in their work environments.
Evaluation of the Inhibitory Effect of Moringa oleifera Leaves Methanolic Extract against In Vitro Growth of Several Babesia Species and Theileria equi and the In Vivo Growth of Babesia microti
Authors: Rizk Mohamed Abdo , El-Sayed Shimaa Abd El-Salam , Sayed-Ahmed Mohamed Z. , Almoshari Yosif , Alqahtani Saad S. , Ahmad Sarfaraz , Alam Nawazish , Marghani Basma H. , Abdelbaset Abdelbaset E. , Igarashi Ikuo
Published: November 2023

The current study evaluated the inhibitory effect of Moringa oleifera leaves methanolic extract (MOL) against the in vitro growth of Babesia bovis (B. bovis), B. caballi, B. bigemina, and Theileria equi (T. equi), as well as in vivo growth of B. microti in mice. Active principles of MOL extract were determined using liquid chromatography mass spectrometry (LC-MS). MOL’s anti-piroplasm efficacy was assessed both in vitro and in vivo using the SYBR Green I fluorescence assay. Every 96 hours, the hematological parameters, including red blood cell count (RBCs; 104/UL), hemoglobin content (HGB; g/dl), and hematocrit percent (HCT; %), in the treated mice were monitored using a Celltac MEK6450 automated hematological analyzer. LC-MS of MOL revealed that the most abundant polyphenolic catechism found in the MOL extract was isoquercetin and rutin. MOL inhibited B. bovis, B. caballi, B. bigemina, and T. equi in vitro growth in a dose-dependent way, with IC50 values of 45.29 ± 6.14, 19.16 ± 0.45, 137.49 ± 16.07, and 9.29 ± 0.014 μg/ml, respectively. MOL’s in vitro antibabesial activity was enhanced when administrated simultaneously with either diminazene aceturate (DA) or MMV665875 compound from malaria box. In mice infected by B. microti, a combination of MOL and a low dose of DA (12.5 mg·kg−1) resulted in a significant (

P
<
0.05

) reduction in B. microti growth. These findings suggest that MOL is an effective herbal anti-piroplasm therapy, especially when combined with a low dosage of either DA or MMV665875.
Spatio-Temporal Change of Land Use/Land Cover and Vegetation Using Multi-MODIS Satellite Data, Western Ethiopia
Authors: Seifu Tesema Kebede , Woldesenbet Tekalegn Ayele , Alemayehu Taye , Ayenew Tenalem
Published: November 2023

Land use and land cover (LULC) change and variability are some of the challenges to present-day water resource management. The purpose of this study was to determine LULC and Normalized Difference Vegetation Index (NDVI) fluctuations in western Ethiopia during the last 20 years. The first part of the study used MODIS LULC data for the change analysis, change detection, and spatial and temporal coverage in the study region. In the second part, the study analyzes the NDVI change and its spatial and temporal coverage. In this study, The Moderate Resolution Imaging Spectroradiometer (MODIS) satellite data were applied to determine LULC and NDVI changes over four different periods. Evergreen broadleaf forests, deciduous broadleaf forests, mixed forests, woody savannas, savannas, grasslands, permanent wetlands, croplands, urban and built-up lands, and water bodies are the LULC in the period of analysis. The overall classification accuracy for the classified image from 2001 to 2020 was 85.4% and the overall kappa statistic was 81.2%. The results indicate a substantial increase in woody savannas, deciduous broadleaf, grasslands, permanent wetlands, and mixed forest areas by 119.6%, 57.7% 45.2%, 37%, and 21.3%, respectively, followed by reductions in croplands, water bodies, savannas, and evergreen broadleaf forest by 90.1%, 19.8%, 13.2%, and 4.8%, respectively, for the catchment between 2001 and 2020. The result also showed that the area’s vegetation cover increased by 64% from 2001 to 2022. This study could provide valuable information for water resource and environmental management as well as policy and decision-making.
Cyclic Behavior of Square CFST Columns-Based Joint
Authors: Nie Ruifeng , Yao Jinfeng , Li Songhui , Wang Shuo , Han Weiwei
Published: November 2023

This paper investigates the cyclic behavior of six square concrete-filled steel tube (CFST) columns that take the effect of width–thickness ratio (D/t = 30, 40, and 60), slenderness ratio (λ = 19.63 and 24.25), and axial force ratio (n = 0.6, 0.4, and 0.2) into consideration. The failure modes, hysteretic behavior, energy dissipation capacities, stiffness degradation, and ductility of these specimens have been discussed and studied. The results revealed that a bigger D/t could reduce the energy dissipation capacities and bearing capacities of the columns. With increasing λ, the energy dissipation capacities and bearing capacities of columns reduced, and the stiffness deteriorated rapidly. The axial force ratio could enhance the bearing capacities but weaken their energy dissipation capacities and stiffness. Calculation formulae for ultimate moment strength of square CFST columns were exhibited to compare to the test results.
Eichhornia crassipes Efficacy in Secondary Wastewater Treatment in the Western Highlands of Cameroon
Authors: Manekeu Tanetsa Amandine Elodie , Lekeufack Martin , Edzigui Tsimi Marcelle Léonce , Tsetagho Gabriel Nicodème , Longniang Raoul Carmel , Fonkou Théophile
Published: November 2023

Wastewater treatment using lagoons technology is recognised to have potential to protect the environment and preserve water resources. The implementation requires mastery of ecological conditions of operation and management of the plants used. We aimed to contribute to the implementation of macrophyte-based lagoons for wastewater treatment in the Western Highlands of Cameroon. We used two sets of four lagoons in series; one was vegetated with E. crassipes, while the other served as the control. Each set constituted an anaerobic, two facultative, and maturation lagoons. Each lagoon system was supplied with primary-treated domestic wastewater from the wastewater treatment plant of the University of Dschang, Cameroon, at a hydraulic loading rate of 1.13 m3/day. Monthly wastewater samples were collected from the inflow and outflow of each lagoon for physicochemical and bacteriological analyses, and plant growth was assessed biweekly for six months spanning the dry and rainy seasons. Macrophyte lagoons showed positive plant growth during both seasons. The highest plant height (10.67 and 25.21 cm), longer roots (11.3 and 37.41 cm), and the highest number of new buds (14 and 20 buds) were obtained during the dry and rainy seasons, respectively. Plants were significantly (

P

 < 0.0001) taller in facultative lagoons in the rainy season than in the dry season. Vegetated lagoons significantly (

P

 < 0.05) contributed to reducing TSS (−08.36% and 82.25%), true color (44.46% and 82.31%), orthophosphates (63.1% and 86.59%), and BOD5 (−56.79% and 56.06%) during the dry and rainy seasons, respectively. 100% elimination of fecal streptococci (FS) and 74.89% and 43.80% of fecal coliforms (FC) were observed in the dry and rainy seasons, respectively. Significant (

P

 = 0.0019) FS elimination was more in the dry season than in the rainy season. However, the average residual content of FC (190,000 UFC/100 ml) was still higher than WHO limits (≤1000 UFC/100 ml) for nonrestrictive reuse in agriculture. E. crassipes can be efficiently used in lagoon systems for domestic wastewater treatment in the Western Highlands of Cameroon.
The Safety and Efficacy of Macrofocused Ultrasound without Visualization on Enlarged Facial Pores among Thai Patients: A Pilot Study
Authors: Wanitphakdeedecha Rungsima , Borjal Kua-Uy Katrina Kashmyr , Yan Chadakan , Nokdhes Ya-Nin , Suphatsathienkul Panittra , Hutachoke Thrit , Viriyaskultorn Noldtawat , Leesanguankul Thanyaporn
Published: November 2023

Introduction. Visibly enlarged facial pores are familiar dermatologic concerns. Macrofocused ultrasound energy without visualization (MaFU‐WV) showed efficacy in facial tightening and an improvement in skin textural irregularities which opened the potential of the positive effects in reducing the appearance of facial pores. This study aims to assess the safety and efficacy of MaFU‐WV in tightening facial pores. Methods. This was a prospective, single‐blinded pilot study. Thirty‐four Thai subjects with enlarged pores received a single treatment of MaFU‐WV using a 2.0 mm transducer on bilateral malar areas of the face. Primary outcome measures included the pore count, pore volume, and pore index measurements using an instrument with a camera for image acquisition and software for analysis of skin. Secondary outcome measures incorporated two blinded dermatologists’ evaluation of clinical photographs and the subjects’ perception of improvement in their facial pores using a 6‐point scale. Measurements were taken at baseline, 1 week, 1 month, 3 month, and 6 month after treatment. Results. The pore count significantly decreased from baseline to 6 month after treatment (p  < 0.001). Pore volume was significantly lowered from baseline to 1 week and 1, 3, and 6 months after treatment (p < 0.05). The pore index was likewise significantly reduced from baseline in every visit (p < 0.05). The majority of photographic evaluations by blinded dermatologists were scored as a 1–25% pore minimizing effect across nearly all follow‐up visits. On the other hand, patient satisfaction kept improving until the end of the study at 6 months. No adverse events occurred throughout the conduct of the study. Conclusion. Macrofocused ultrasound energy using a 2 mm transducer is a safe and effective treatment for facial pore tightening. The trial is registered with registration number: TCTR20220719001.
Continuous-Variable Pairwise Entanglement Based on Optoelectromechanical System
Authors: Cai Qizhi , Liao Jinkun , Zhou Qiang
Published: November 2023

We show how to generate stationary continuous-variable pairwise entanglement between microwave modes in a hybrid optoelectromechanical system, which consists of a single Fabry–Pérot cavity, a parallel-plate capacitor with a moving element as the mechanical resonator, and several pairs of microwave cavities. The optical mode and mechanical resonator are coupled via radiation pressure; meanwhile, several pairs of the microwave mode and mechanical resonator are capacitively coupled. Under an experimentally reachable parameter regime, we show the influence of different key parameters on pairwise entanglement and find that it is also robust against temperature. Our model and results are expected to provide a new perspective on quantum networks with increasingly large scales, quantum internet with multiple local users, and multiport microwave quantum illumination radar.
The Development of Eco-Friendly Biopolymers for Use in Tissue Engineering and Drug Delivery
Authors: Mahmud Md. Zobair Al , Islam Md. Didarul , Mobarak Md Hosne
Published: November 2023

Tissue engineering and drug delivery have emerged as promising fields that hold the potential to revolutionize modern healthcare. The widespread use of synthetic polymers in these applications raises concerns about their environmental impact and biocompatibility. In response, researchers have shifted their focus toward the development of eco-friendly biopolymers as viable alternatives. This review paper aims to comprehensively analyze the progress made in the utilization of biopolymers for tissue engineering and drug delivery, with an emphasis on their ecological advantages and biomedical performance. The review begins by exploring the diverse sources of biopolymers, such as proteins, polysaccharides, and nucleic acids, derived from renewable resources, agricultural waste, etc. The discussion then delves into the unique properties of these biopolymers, including their biodegradability, nontoxic nature, and potential for controlled drug release. The incorporation of biopolymers in tissue engineering scaffolds is critically examined. The review also explores the role of biopolymers in the drug delivery systems, with a focus on nanoparticles, microparticles, hydrogels, and films. The capability of biopolymers to encapsulate and protect therapeutic agents, as well as their controlled release, is discussed in the context of targeted drug delivery and improved therapeutic outcomes. This paper highlights the challenges and future perspectives in the field of eco-friendly biopolymers for tissue engineering and drug delivery. Promising advancements in biofabrication techniques, nanotechnology, and personalized medicine are identified as key drivers for the rapid translation of these materials into the clinical applications. The development of eco-friendly biopolymers offers a sustainable and bio-safe alternative to the traditional synthetic polymers in tissue engineering and drug delivery. This comprehensive review underscores the crucial role of biopolymers in fostering advancements in regenerative medicine and therapeutic interventions while prioritizing environmental preservation and human health.
Table S4 from Matrix Stiffness Triggers Lipid Metabolic Cross-talk between Tumor and Stromal Cells to Mediate Bevacizumab Resistance in Colorectal Cancer Liver Metastases
Authors: Zheng Yannan , Zhou Rui , Cai Jianan , Yang Nanyan , Wen Zhaowei , Zhang Zhihua , Sun Huiying , Huang Genjie , Guan Yijin , Huang Na , Shi Min , Liao Yulin , Bin Jianping , Liao Wangjun
Published: November 2023

Supplementary Table S4. Primer sequences
Figure S3 from Matrix Stiffness Triggers Lipid Metabolic Cross-talk between Tumor and Stromal Cells to Mediate Bevacizumab Resistance in Colorectal Cancer Liver Metastases
Authors: Zheng Yannan , Zhou Rui , Cai Jianan , Yang Nanyan , Wen Zhaowei , Zhang Zhihua , Sun Huiying , Huang Genjie , Guan Yijin , Huang Na , Shi Min , Liao Yulin , Bin Jianping , Liao Wangjun
Published: November 2023

Figure. S3 Conditioned medium derived from HSCs cultured on the stiff substrates upregulates FAO metabolism in colon cancer cells.
Table S9 from Cross-talk between Myeloid and B Cells Shapes the Distinct Microenvironments of Primary and Secondary Liver Cancer
Authors: Chen Zhihang , Zhang Guopei , Ren Xiaoxue , Yao Zhijia , Zhou Qian , Ren Xuxin , Chen Shuling , Xu Lixia , Sun Kaiyu , Zeng Qianwen , Kuang Ming , Kuang Dong-Ming , Peng Sui
Published: November 2023

The RNA sequencing count data of 61 HCC and 62 CRLM tissues
Data from Biallelic Dicer1 Mutations in the Gynecologic Tract of Mice Drive Lineage-Specific Development of DICER1 Syndrome–Associated Cancer
Authors: Wang Yemin , Chen Shary Yuting , Ta Monica , Senz Janine , Tao Lan Valerie , Thornton Shelby , Tamvada Nirupama , Yang Winnie , Moscovitz Yana , Li Eunice , Guo Jingjie , Shen Cindy , Douglas J. Maxwell , EI-Naggar Amal M. , Kommoss Felix K.F. , Underhill T. Michael , Singh Naveena , Gilks C. Blake , Morin Gregg B. , Huntsman David G.
Published: November 2023

Abstract
DICER1 is an RNase III enzyme essential for miRNA biogenesis through cleaving precursor-miRNA hairpins. Germline loss-of-function DICER1 mutations underline the development of DICER1 syndrome, a rare genetic disorder that predisposes children to cancer development in organs such as lung, gynecologic tract, kidney, and brain. Unlike classical tumor suppressors, the somatic “second hit” in DICER1 syndrome–associated cancers does not fully inactivate DICER1 but impairs its RNase IIIb activity only, suggesting a noncanonical two-hit hypothesis. Here, we developed a genetically engineered conditional compound heterozygous Dicer1 mutant mouse strain that fully recapitulates the biallelic DICER1 mutations in DICER1 syndrome–associated human cancers. Crossing this tool strain with tissue-specific Cre strains that activate Dicer1 mutations in gynecologic tract cells at two distinct developmental stages revealed that embryonic biallelic Dicer1 mutations caused infertility in females by disrupting oviduct and endometrium development and ultimately drove cancer development. These multicystic tubal and intrauterine tumors histologically resembled a subset of DICER1 syndrome–associated human cancers. Molecular analysis uncovered accumulation of additional oncogenic events (e.g., aberrant p53 expression, Kras mutation, and Myc activation) in murine Dicer1 mutant tumors and validated miRNA biogenesis defects in 5P miRNA strand production, of which, loss of let-7 family miRNAs was identified as a putative key player in transcriptomic rewiring and tumor development. Thus, this DICER1 syndrome–associated cancer model recapitulates the biology of human cancer and provides a unique tool for future investigation and therapeutic development.
Significance:
Generation of a Dicer1 mutant mouse model establishes the oncogenicity of missense mutations in the DICER1 RNase IIIb domain and provides a faithful model of DICER1 syndrome–associated cancer for further investigation.
Supplementary Table S3 from Assessing a Polygenic Risk Score for Lung Cancer Susceptibility in Non-Hispanic White and Black Populations
Authors: Trendowski Matthew R. , Lusk Christine M. , Wenzlaff Angela S. , Neslund-Dudas Christine , Gadgeel Shirish M. , Soubani Ayman O. , Schwartz Ann G.
Published: November 2023

This supplementary table indicates the association between SNPs in the PRS and smoking intensity.
Investigating Causal Effects of Hematologic Traits on Lung Cancer: A Mendelian Randomization Study
Authors: Yang Zhanghuan , He Hao , He Guangxu , Zeng Chudai , Hu Qian
Published: November 2023

Background:

Observational studies have suggested blood cell counts may act as predictors of cancer. It is not known whether these hematologic traits are causally associated with lung cancer.

Methods:

Two-sample bidirectional univariable Mendelian randomization (MR) and multivariable MR (MVMR) were performed to investigate the causal association between hematologic traits and the overall risk of lung cancer and three histologic subtypes [lung adenocarcinoma, squamous cell lung cancer, and small cell lung cancer (SCLC)]. The instrumental variables of 23 hematologic traits were strictly selected from large-scale genome-wide association studies. Inverse-variance weighted method and five extra methods were used to obtain robust causal estimates.

Results:

We found evidence that genetically influenced higher hematocrit [OR, 0.845; 95% confidence interval (CI), 0.783–0.913; P = 1.68 × 10−5] and hemoglobin concentration (OR, 0.868; 95% CI, 0.804–0.938; P = 3.20 × 10−4) and reticulocyte count (OR, 0.923; 95% CI, 0.872–0.976; P = 5.19 × 10−3) decreased lung carcinoma risk, especially in ever smokers. MVMR further identified hematocrit independently of smoking as an independent predictor. Subgroup analysis showed that a higher plateletcrit level increased the risk of small cell lung carcinoma (OR, 1.288; 95% CI, 1.126–1.474; P = 2.25 × 10−4).

Conclusions:

Genetically driven higher levels of reticulocyte count and hematocrit decreased lung cancer risk. Higher plateletcrit had an adverse effect on SCLC. Hematologic traits may act as low-cost factors for lung cancer risk stratification.

Impact:

Further studies are required to elucidate the potential mechanisms underlying the dysregulation of homeostasis related to hematologic traits, such as subclinical inflammation.
Supplementary Figure S8 from Exercise Training Reduces the Inflammatory Response and Promotes Intestinal Mucosa-Associated Immunity in Lynch Syndrome
Authors: Deng Nan , Reyes-Uribe Laura , Fahrmann Johannes F. , Thoman Whittney S. , Munsell Mark F. , Dennison Jennifer B. , Murage Eunice , Wu Ranran , Hawk Ernest T. , Thirumurthi Selvi , Lynch Patrick M. , Dieli-Conwright Christina M. , Lazar Alexander J. , Jindal Sonali , Chu Khoi , Chelvanambi Manoj , Basen-Engquist Karen , Li Yisheng , Wargo Jennifer A. , McAllister Florencia , Allison James P. , Sharma Padmanee , Sinha Krishna M. , Hanash Samir , Gilchrist Susan C. , Vilar Eduardo
Published: November 2023

Figure S8. (A) Volcano plot of statistically significant genes and fold-changes between the usual care and exercise groups in DSP assay. Top significantly differentially expressed genes are labeled; (B) The dot plot presents enriched GSEA HALLMARK pathways. The sizes of the dots represent the count of core enrichment (leading-edge) genes. The colors of the dots represent the BH-adjusted P-values. The order of HALLMARK gene sets is based on the gene ratio (number of significant genes associated with the HALLMARK gene sets / total number of significant genes; (C) Total CD8+ T cell density per mm2 (left) and epithelial (right) in colonic mucosa preand post- intervention from usual care (n=4) and exercise (n=4) participants; (D) Total CD57+ NK cell density per mm2 (left) and epithelial (right) in colonic mucosa pre- and post- intervention from usual care (n=4) and exercise (n=4) participants.
Supplementary Figure S7 from SPOP Mutations Target STING1 Signaling in Prostate Cancer and Create Therapeutic Vulnerabilities to PARP Inhibitor–Induced Growth Suppression
Authors: Geng Chuandong , Zhang Man-Chao , Manyam Ganiraju C. , Vykoukal Jody V. , Fahrmann Johannes F. , Peng Shan , Wu Cheng , Park Sanghee , Kondraganti Shakuntala , Wang Daoqi , Robinson Brian D. , Loda Massimo , Barbieri Christopher E. , Yap Timothy A. , Corn Paul G. , Hanash Samir , Broom Bradley M. , Pilié Patrick G. , Thompson Timothy C.
Published: November 2023

Supplementary Fig. S7. Original unedited IB images for representative Western blots experiments (n-2) used in Supplementary Figure S6.
Data from Clinical and Molecular Features of Long-term Response to Immune Checkpoint Inhibitors in Patients with Advanced Non–Small Cell Lung Cancer
Authors: Thummalapalli Rohit , Ricciuti Biagio , Bandlamudi Chaitanya , Muldoon Daniel , Rizvi Hira , Elkrief Arielle , Luo Jia , Alessi Joao V. , Pecci Federica , Lamberti Giuseppe , Di Federico Alessandro , Hong Lingzhi , Zhang Jianjun , Heymach John V. , Gibbons Don L. , Plodkowski Andrew J. , Ravichandran Vignesh , Donoghue Mark T.A. , Vanderbilt Chad , Ladanyi Marc , Rudin Charles M. , Kris Mark G. , Riely Gregory J. , Chaft Jamie E. , Hellmann Matthew D. , Vokes Natalie I. , Awad Mark M. , Schoenfeld Adam J.
Published: November 2023

AbstractPurpose:
We sought to identify features of patients with advanced non–small cell lung cancer (NSCLC) who achieve long-term response (LTR) to immune checkpoint inhibitors (ICI), and how these might differ from features predictive of short-term response (STR).
Experimental Design:
We performed a multicenter retrospective analysis of patients with advanced NSCLC treated with ICIs between 2011 and 2022. LTR and STR were defined as response ≥ 24 months and response < 12 months, respectively. Tumor programmed death ligand 1 (PD-L1) expression, tumor mutational burden (TMB), next-generation sequencing (NGS), and whole-exome sequencing (WES) data were analyzed to identify characteristics enriched in patients achieving LTR compared with STR and non-LTR.
Results:
Among 3,118 patients, 8% achieved LTR and 7% achieved STR, with 5-year overall survival (OS) of 81% and 18% among LTR and STR patients, respectively. High TMB (≥50th percentile) enriched for LTR compared with STR (P = 0.001) and non-LTR (P < 0.001). Whereas PD-L1 ≥ 50% enriched for LTR compared with non-LTR (P < 0.001), PD-L1 ≥ 50% did not enrich for LTR compared with STR (P = 0.181). Nonsquamous histology (P = 0.040) and increasing depth of response [median best overall response (BOR) −65% vs. −46%, P < 0.001] also associated with LTR compared with STR; no individual genomic alterations were uniquely enriched among LTR patients.
Conclusions:
Among patients with advanced NSCLC treated with ICIs, distinct features including high TMB, nonsquamous histology, and depth of radiographic improvement distinguish patients poised to achieve LTR compared with initial response followed by progression, whereas high PD-L1 does not.
Supplementary Data S1. from Efficacy and Safety of Glycosphingolipid SSEA-4 Targeting CAR-T Cells in an Ovarian Carcinoma Model
Authors: Monzo Hector J. , Kalander Kerttu , Hyytiäinen Marko M. , Elbasani Endrit , Wall Johanna , Moyano-Galceran Lidia , Tanjore Ramanathan Jayendrakishore , Jukonen Joonas , Laakkonen Pirjo , Ristimäki Ari , Carlson Joseph W. , Lehti Kaisa , Salehi Sahar , Puolakkainen Pauli , Haglund Caj , Seppänen Hanna , Leppä Sirpa , Ojala Päivi M.
Published: November 2023

Supplementary Data S1. Pathology Report of the long-term survival study. Post-mortem lung, intestine and kidney tissue samples collected and analyzed by Histowiz.
Data from Targeted Investigational Oncology Agents (IOA) in the NCI60: A Phenotypic Systems-Based Resource
Authors: Morris Joel , Kunkel Mark W. , White Stephen L. , Wishka Donn G. , Lopez Omar D. , Bowles Lori , Sellers Brady Penny , Ramsey Patricia , Grams Julie , Rohrer Tiffany , Martin Karen , Dexheimer Thomas S. , Coussens Nathan P. , Evans David , Risbood Prabhakar , Sonkin Dmitriy , Williams John D. , Polley Eric C. , Collins Jerry M. , Doroshow James H. , Teicher Beverly A.
Published: November 2023

Abstract
The NCI-60 human tumor cell line panel has proved to be a useful tool for the global cancer research community in the search for novel chemotherapeutics. The publicly available cell-line characterization and compound screening data from the NCI-60 assay have significantly contributed to the understanding of cellular mechanisms targeted by new oncology agents. Signature sensitivi-ty/resistance patterns generated for a given chemotherapeutic agent against the NCI-60 panel have long served as fingerprint presentations that encompass target information and the mechanism of action associated with the tested agent. We report the establishment of a new public NCI-60 resource based on the cell-line screening of a large and growing set of 175 FDA-approved oncolo-gy drugs (AOD) plus >825 clinical and investigational oncology agents (IOA), representing a diverse set (>250) of therapeutic targets and mechanisms. This data resource is available to the public (https://ioa.cancer.gov) and includes the raw data from the screening of the IOA and AOD collection along with an ex-tensive set of visualization and analysis tools to allow for comparative study of individual test compounds and multiple compound sets.
Data from Efficacy and Safety of Glycosphingolipid SSEA-4 Targeting CAR-T Cells in an Ovarian Carcinoma Model
Authors: Monzo Hector J. , Kalander Kerttu , Hyytiäinen Marko M. , Elbasani Endrit , Wall Johanna , Moyano-Galceran Lidia , Tanjore Ramanathan Jayendrakishore , Jukonen Joonas , Laakkonen Pirjo , Ristimäki Ari , Carlson Joseph W. , Lehti Kaisa , Salehi Sahar , Puolakkainen Pauli , Haglund Caj , Seppänen Hanna , Leppä Sirpa , Ojala Päivi M.
Published: November 2023

Abstract
Chimeric antigen receptor (CAR) T-cell immunotherapies for solid tumors face critical challenges such as heterogeneous antigen expression. We characterized stage-specific embryonic antigen-4 (SSEA-4) cell-surface glycolipid as a target for CAR T-cell therapy. SSEA-4 is mainly expressed during embryogenesis but is also found in several cancer types making it an attractive tumor-associated antigen. Anti-SSEA-4 CAR-T cells were generated and assessed preclinically in vitro and in vivo for antitumor response and safety. SSEA-4 CAR-T cells effectively eliminated SSEA-4–positive cells in all the tested cancer cell lines, whereas SSEA-4–negative cells lines were not targeted. In vivo efficacy and safety studies using NSG mice and the high-grade serous ovarian cancer cell line OVCAR4 demonstrated a remarkable and specific antitumor response at all the CAR T-cell doses used. At high T-cell doses, CAR T cell–treated mice showed signs of health deterioration after a follow-up period. However, the severity of toxicity was reduced with a delayed onset when lower CAR T-cell doses were used. Our data demonstrate the efficacy of anti-SSEA-4 CAR T-cell therapy; however, safety strategies, such as dose-limiting and/or equipping CAR-T cells with combinatorial antigen recognition should be implemented for its potential clinical translation.
Data from Exon Junction Complex Mediates the Cap-Independent Translation of Circular RNA
Authors: Lin Hui-Hsuan , Chang Chiu-Yuan , Huang Yi-Ren , Shen Che-Hung , Wu Yu-Chen , Chang Kai-Li , Lee Yueh-Chun , Lin Ya-Chi , Ting Wen-Chien , Chien Han-Ju , Zheng Yi-Feng , Lai Chien-Chen , Hsiao Kuei-Yang
Published: November 2023

Abstract
Evidence that circular RNAs (circRNA) serve as protein template is accumulating. However, how the cap-independent translation is controlled remains largely uncharacterized. Here, we show that the presence of intron and thus splicing promote cap-independent translation. By acquiring the exon junction complex (EJC) after splicing, the interaction between circRNA and ribosomes was promoted, thereby facilitating translation. Prevention of splicing by treatment with spliceosome inhibitor or mutating splicing signal hindered cap-independent translation of circRNA. Moreover, EJC-tethering using Cas13 technology reconstituted EJC-dependent circRNA translation. Finally, the level of a coding circRNA from succinate dehydrogenase assembly factor 2 (circSDHAF2) was found to be elevated in the tumorous tissues from patients with colorectal cancer, and shown to be critical in tumorigenesis of colorectal cancer in both cell and murine models. These findings reveal that EJC-dependent control of circSDHAF2 translation is involved in the regulation of oncogenic pathways.
Implications:
EJC-mediated cap-independent translation of circRNA is implicated in the tumorigenesis of colorectal cancer.
Data from Stromal DDR2 promotes ovarian cancer metastasis through regulation of metabolism and secretion of extracellular matrix proteins
Authors: Schab Angela M. , Greenwade Molly M. , Stock Elizabeth , Lomonosova Elena , Cho Kevin , Grither Whitney R. , Noia Hollie , Wilke Daniel , Mullen Mary M. , Hagemann Andrea R. , Hagemann Ian S. , Thaker Premal H. , Kuroki Lindsay M. , McCourt Carolyn K. , Khabele Dineo , Powell Matthew A. , Mutch David G. , Zhao Peinan , Shriver Leah P. , Patti Gary J. , Longmore Gregory D. , Fuh Katherine C.
Published: November 2023

Abstract
Ovarian cancer is the leading cause of gynecological cancer-related deaths. The propensity for metastasis within the peritoneal cavity is a driving factor for the poor outcomes associated with this disease, but there is currently no effective therapy targeting metastasis. In this study we investigate the contribution of stromal cells to ovarian cancer metastasis and identify normal stromal cell expression of the collagen receptor, Discoidin Domain Receptor 2 (DDR2), that acts to facilitate ovarian cancer metastasis. In vivo, global genetic inactivation of Ddr2 impairs the ability of Ddr2-expressing syngeneic ovarian cancer cells to spread throughout the peritoneal cavity. Specifically, DDR2 expression in mesothelial cells lining the peritoneal cavity facilitates tumor cell attachment and clearance. Subsequently, omentum fibroblast expression of DDR2 promotes tumor cell invasion. Mechanistically, we find DDR2-expressing fibroblasts are more energetically active, such that DDR2 regulates glycolysis through AKT/SNAI1 leading to suppressed fructose-1,6-bisphosphatase and increased hexokinase activity, a key glycolytic enzyme. Upon inhibition of DDR2 we find decreased protein synthesis and secretion. Consequently, when DDR2 is inhibited, there is reduction in secreted extracellular matrix proteins important for metastasis. Specifically, we find that fibroblast DDR2 inhibition leads to decreased secretion of the collagen crosslinker, LOXL2. Adding back LOXL2 to DDR2 deficient fibroblasts rescues the ability of tumor cells to invade. Overall, our results suggest that stromal cell expression of DDR2 is an important mediator of ovarian cancer metastasis.

Implications: DDR2 is highly expressed by stromal cells in ovarian cancer that can mediate metastasis and is a potential therapeutic target in ovarian cancer.
Supplementary Table 2 from Polymorphisms in Cyclooxygenase, Lipoxygenase, and TP53 Genes Predict Colorectal Polyp Risk Reduction by Aspirin in the seAFOod Polyp Prevention Trial
Authors: Davies John R. , Mell Tracey , Fuller Harriett , Harland Mark , Saleh Rasha N.M. , Race Amanda D. , Rees Colin J. , Brown Louise C. , Loadman Paul M. , Downing Amy , Minihane Anne Marie , Williams Elizabeth A. , Hull Mark A.
Published: November 2023

Supplementary Table 2 shows the results of Negative binomial regression analysis of total colorectal polyp number according to aspirin use stratified for each SNP
Supplementary Table S22 from Transposable Elements Are Co-opted as Oncogenic Regulatory Elements by Lineage-Specific Transcription Factors in Prostate Cancer
Authors: Grillo Giacomo , Keshavarzian Tina , Linder Simon , Arlidge Christopher , Mout Lisanne , Nand Ankita , Teng Mona , Qamra Aditi , Zhou Stanley , Kron Ken J. , Murison Alex , Hawley James R. , Fraser Michael , van der Kwast Theodorus H. , Raj Ganesh V. , He Housheng Hansen , Zwart Wilbert , Lupien Mathieu
Published: November 2023

Top 5% transcription factor cistromes enriched at reprogrammed transposable element families (CPC-GENE constant patients)