bluuebunny/crossref_metadata_2025 huggingface dataset:
Prediction of extracapsular extension in prostate cancer using the Likert scale combined with clinical and pathological parameters
Authors: Wang Jun-guang , Huang Bin-tian , Huang Li , Zhang Xia , He Pei-pei , Chen Jun-bo
Published: August 2023

This study aimed to investigate the independent clinical, pathological, and radiological factors associated with extracapsular extension in radical prostatectomy specimens and to improve the accuracy of predicting extracapsular extension of prostate cancer before surgery.

Methods

From August 2018 to June 2023, the clinical and pathological data of 229 patients with confirmed prostate cancer underwent radical prostatectomy from The Second Hospital of Yinzhou. The patients’ multiparametric magnetic resonance imaging data were graded using the Likert scale. The chi-square or independent-sample T-test was used to analyze the related factors for an extracapsular extension. Multivariate analysis was used to identify independent factors associated with extracapsular extension in prostate cancer. Additionally, receiver operating characteristic curve analysis was used to calculate the area under the curve and assess the diagnostic performance of our model. The clinical decision curve was used to analyze the clinical net income of Likert scale, biopsy positive rate, biopsy GG, and combined mode.

Results

Of the 229 patients, 52 had an extracapsular extension, and 177 did not. Multivariate analysis showed that the Likert scale score, biopsy grade group and biopsy positive rate were independent risk factors for extracapsular extension in prostate cancer. The area under the curves for the Likert scale score, biopsy grade group, and biopsy positive rate were 0.802, 0.762, and 0.796, respectively. Furthermore, there was no significant difference in the diagnostic efficiency for extracapsular extension (P>0.05). However, when these three factors were combined, the diagnostic efficiency was significantly improved, and the area under the curve increased to 0.905 (P<0.05). In the analysis of the decision curve, The clinical net income of the combined model is obviously higher than that of Likert scale, biopsy positive rate, and biopsy GG.

Conclusion

The Likert scale, biopsy grade group and biopsy positive rate are independent risk factors for extracapsular extension in prostate cancer, and their combination can significantly improve the diagnostic efficiency for an extracapsular extension.
Medication overuse headache: a review of current evidence and management strategies
Authors: Kebede Yabets Tesfaye , Mohammed Bekri Delil , Tamene Beimnet Ayenew , Abebe Abel Tezera , Dhugasa Rabbi Waqshum
Published: August 2023

The third edition of the International Classification of Headache Disorders (ICHD-3) defines medication-overuse headache (MOH) as a headache that develops when a person regularly uses acute or symptomatic headache medications excessively (10 or more, or 15 or more days per month, depending on the medication) for a period of time longer than 3 months. Even though it may not be reported as frequently as it actually is, it affects about 5% of the general population on average. It typically happens following repeated anti-pain medication use for pre-existing headache disorders, such as migraines. Anti-pains can also be used frequently in patients with pre-existing headache disorders for reasons other than treating headaches, such as psychological drug attachment. MOH is linked to a number of illnesses, such as anxiety, depression, and obsessive compulsive disorder (OCD). Both simple and complex types are possible. Additionally, there is no universal consensus on how to treat MOH, but drug discontinuation is the best course of action. Using the medical subject headings “Medication Overuse Headache,” “Migraine Headache,” “Tension Headache,” “Chronification of Headache,” and “Antipains,” an all-language literature search was done on PubMed, Google Scholar, and Medline up until March 2023. We looked into the epidemiology, risk factors, pathophysiology, clinical characteristics, comorbidities, diagnosis, management, and preventative measures of MOH in the literature. This article focuses on the MOH research themes.
Case report: Immunotherapy plus chemotherapy and stereotactic ablative radiotherapy (ICSABR): a novel treatment combination for Epstein-Barr virus-associated lymphoepithelioma-like intrahepatic cholangiocarcinoma
Authors: Li Ruizhen , Cheng Ke , Li Xiaofen , Chang Chen , Lv Wanrui , Xiaoying Li , Zhang Pei , Yang Heqi , Cao Dan
Published: August 2023

Epstein-Barr virus-associated lymphoepithelioma-like intrahepatic cholangiocarcinoma (EBVa LEL-ICC) is a rare tumor, characterized by a rich tumor immune microenvironment (TIME). While this tumor is reportedly sensitive to immunotherapy, its response has been inconsistent. This decreased sensitivity was associated with reduced TIME abundance. We report the case of a 53-year-old woman with EBVa LEL-ICC having reduced TIME abundance. The patient presented with a liver lesion, which was detected using ultrasound. Initially, the tumor was sensitive to immunotherapy and chemotherapy (IC), but resistance developed after a short interval. Subsequently, stereotactic ablative radiotherapy (SABR) was added to the patient’s treatment, which now consisted of ICSABR. Successful tumor shrinkage was achieved with the combination therapy regimen. Thus, surgery and ICSABR are effective adjuncts to the first-line IC therapy in improving the survival rate of patients with EBVa LEL-ICC. The results of this study support multidisciplinary treatment as a viable treatment strategy for EBVa LEL-ICC.
DMC1 stabilizes crossovers at high and low temperatures during wheat meiosis
Authors: Draeger Tracie N. , Rey María-Dolores , Hayta Sadiye , Smedley Mark , Martin Azahara C. , Moore Graham
Published: August 2023

Effective chromosome synapsis and crossover formation during meiosis are essential for fertility, especially in grain crops such as wheat. These processes function most efficiently in wheat at temperatures between 17-23 °C, although the genetic mechanisms for such temperature dependence are unknown. In a previously identified mutant of the hexaploid wheat reference variety ‘Chinese Spring’ lacking the long arm of chromosome 5D, exposure to low temperatures during meiosis resulted in asynapsis and crossover failure. In a second mutant (ttmei1), containing a 4 Mb deletion in chromosome 5DL, exposure to 13 °C led to similarly high levels of asynapsis and univalence. Moreover, exposure to 30 °C led to a significant, but less extreme effect on crossovers. Previously, we proposed that, of 41 genes deleted in this 4 Mb region, the major meiotic gene TaDMC1-D1 was the most likely candidate for preservation of synapsis and crossovers at low (and possibly high) temperatures. In the current study, using RNA-guided Cas9, we developed a new Chinese Spring CRISPR mutant, containing a 39 bp deletion in the 5D copy of DMC1, representing the first reported CRISPR-Cas9 targeted mutagenesis in Chinese Spring, and the first CRISPR mutant for DMC1 in wheat. In controlled environment experiments, wild-type Chinese Spring, CRISPR dmc1-D1 and backcrossed ttmei1 mutants were exposed to either high or low temperatures during the temperature-sensitive period from premeiotic interphase to early meiosis I. After 6-7 days at 13 °C, crossovers decreased by over 95% in the dmc1-D1 mutants, when compared with wild-type plants grown under the same conditions. After 24 hours at 30 °C, dmc1-D1 mutants exhibited a reduced number of crossovers and increased univalence, although these differences were less marked than at 13 °C. Similar results were obtained for ttmei1 mutants, although their scores were more variable, possibly reflecting higher levels of background mutation. These experiments confirm our previous hypothesis that DMC1-D1 is responsible for preservation of normal crossover formation at low and, to a certain extent, high temperatures. Given that reductions in crossovers have significant effects on grain yield, these results have important implications for wheat breeding, particularly in the face of climate change.
Transcriptome analysis revealed differentially expressed genes in rice functionally associated with brown planthopper defense in near isogenic lines pyramiding BPH14 and BPH15
Authors: Hu Liang , Yang Dabing , Wang Hongbo , Du Xueshu , Zhang Yanming , Niu Liping , Wan Bingliang , Xia Mingyuan , Qi Huaxiong , Mou Tongmin , You Aiqing , Li Jinbo
Published: August 2023

Although rice has many pests, brown planthopper (BPH) in particular is known to cause substantial damage. The pyramiding application of BPH-resistance genes BPH14 and BPH15 has proven effective in enhancing rice defense against BPH. However, the molecular mechanisms underlying BPH14/BPH15-conferred resistance remain unexplained. In this investigation, we analyzed the transcriptomes of near isogenic lines (NILs) containing either BPH14 (B14), BPH15 (B15), or BPH14/BPH15 (B1415), as well as their recurrent parent (RP) ‘Wushansimiao’. In total, we detected 14,492 differentially expressed genes (DEGs) across 12 mRNA profiles of resistant NILs and RP at different feeding stages. In the transcriptomic analysis, 531 DEGs appeared to be common among the resistant NILs compared to RP before and after BPH feeding. These common DEGs were enriched in defense response, phosphorylation, and salt stress response. In addition, 258 DEGs shared only in resistant NILs were obtained among the different feeding stages, which were enriched in oxidative stress response, karrikin response, and chloroplast organization. Considering the expression patterns and relevant research reports associated with these DEGs, 21 were chosen as BPH resistance candidates. In rice protoplasts, the candidate DEG OsPOX8.1 was confirmed to increase reactive oxygen species (ROS) accumulation by chemiluminescence measurement. Our results provide valuable information to further explore the defense mechanism of insect-resistant gene pyramiding lines and develop robust strategies for insect control.
Sexual development in ADHD and internet pornography consumption
Authors: Privara Michal , Bob Petr
Published: August 2023

Development of sexual identity during adolescence is a major process of transition in individual life which in cases of ADHD specifically interacts with other ontogenetic, pathological and psychosocial conditions. According to recent findings growing consumption of internet pornography mainly in male ADHD population is closely related to compulsive sexual behavior and hypersexuality. Recent findings also indicate that consumption of internet pornography in ADHD individuals and other sexual activities may serve as a mood-altering “self-medication” which may help to cope with stressful events and decrease depression and anxiety. Taken together recent findings indicate that internet pornography consumption mainly in ADHD individuals is closely related to stressful experiences, anxiety, depression and identity problems in partnerships which significantly increase their vulnerability to the so-called “problematic pornography use” and other forms of addictive sexual behavior. From this developmental perspective “problematic pornography use” in ADHD individuals represents significant epidemiological problem which requires further research mainly with focus on clinical diagnostics and treatment.
The effects of climate change-induced flooding on harvest failure in Burkina Faso: case study
Authors: Müller Charlotte , Ouédraogo Windpanga Aristide , Schwarz Maximilian , Barteit Sandra , Sauerborn Rainer
Published: August 2023

Background

Climate change leads to more frequent and severe extreme weather events including floods, heatwaves, heavy rainfalls, and droughts. In contrast to the majority of research on weather extremes in sub-Saharan Africa, which focus primarily on how a lack of rainfall causes droughts, this paper aims to elucidate the effect of flooding on harvest failure in rural Burkina Faso.

Methods

We conducted a case study in north-western Nouna, Burkina Faso, between August and December 2021 covering a study population of n = 180 participants. The study comprised four components: (i) interviews with farmers (n = 180) on whether any of their fields had been inundated and if so, on harvest loss on these fields; (ii) determining the feasibility of using Sentinel-2 satellite images to validate study participants reports of floods; (iii) characterizing short-term weather including frequency and duration, of extreme rainfall events within the study area, as well as comparing cumulative rainfall (long-term) over the past 50 years; and (v), estimating both the food energy and economic loss of harvest failure due to flooding.

Results

49% of interviewed farmers (n = 88) reported that floods had damaged at least one of their fields. Some fields (n = 13, 7%) had no harvest due to flooding, while some farmers (n = 14, 8%) had lost part of their harvest. Images from the Sentinel-2-Satellite indicated that reported and remotely observed flooding were consistent. According to time series of data from the local weather station, there has been an increase irregular rainfall distribution and at the same time of cumulative annual rainfall in Nouna. Furthermore, a first illustrative calculation allowed us to estimate the amount of energy lost when one hectare of a common crop is flooded.

Conclusion

This case study demonstrated that flood-related harvest failures leading to crop losses in sub-Saharan Africa, exemplified by Burkina Faso, are likely to be substantial. This study serves as a proof-of-principle for flooding effects on food security. This could provide more detail for agricultural adaptation and mitigation strategies. Inundation-vulnerable fields need alternative and novel management practices, which may only be effectively implemented if agricultural institutions and national policy-making bodies receive evidence of flooding e.g., from remote sensing.
Deep-learning model for predicting physical fitness in possible sarcopenia: analysis of the Korean physical fitness award from 2010 to 2023
Authors: Bae Jun-Hyun , Seo Ji-won , Kim Dae Young
Published: August 2023

Introduction

Physical fitness is regarded as a significant indicator of sarcopenia. This study aimed to develop and evaluate a deep-learning model for predicting the decline in physical fitness due to sarcopenia in individuals with potential sarcopenia.

Methods

This study used the 2010–2023 Korean National Physical Fitness Award data. The data comprised exercise- and health-related measurements in Koreans aged >65 years and included body composition and physical fitness variables. Appendicular muscle mass (ASM) was calculated as ASM/height2 to define normal and possible sarcopenia. The deep-learning model was created with EarlyStopping and ModelCheckpoint to prevent overfitting and was evaluated using stratified k-fold cross-validation (k = 5). The model was trained and tested using training data and validation data from each fold. The model’s performance was assessed using a confusion matrix, receiver operating characteristic curve, and area under the curve. The average performance metrics obtained from each cross-validation were determined. For the analysis of feature importance, SHAP, permutation feature importance, and LIME were employed as model-agnostic explanation methods.

Results

The deep-learning model proved effective in distinguishing from sarcopenia, with an accuracy of 87.55%, precision of 85.57%, recall of 90.34%, and F1 score of 87.89%. Waist circumference (WC, cm), absolute grip strength (kg), and body fat (BF, %) had an influence on the model output. SHAP, LIME, and permutation feature importance analyses revealed that WC and absolute grip strength were the most important variables. WC, figure-of-8 walk, BF, timed up-and-go, and sit-and-reach emerged as key factors for predicting possible sarcopenia.

Conclusion

The deep-learning model showed high accuracy and recall with respect to possible sarcopenia prediction. Considering the need for the development of a more detailed and accurate sarcopenia prediction model, the study findings hold promise for enhancing sarcopenia prediction using deep learning.
Genetic effects of chemically and biosynthesized titanium dioxide nanoparticles in vitro and in vivo of female rats and their fetuses
Authors: Kamal Zeinab , Said Alaa H. , Ebnalwaled A. A. , Rehan Ibrahim F. , Zigo František , Farkašová Zuzana , Allam Mohammad
Published: August 2023

With the increase in nanoparticles (NPs) products on the market, the possibility of animal and human exposure to these materials will increase. The smaller size of NPs facilitates their entrance through placental barriers and allows them to accumulate in embryonic tissue, where they can then be a source of different developmental malformations. Several toxicity studies with chemically synthesized titanium dioxide NPs (CTiO2 NPs) have been recently carried out; although there is insufficient data on exposure to biosynthesized titanium dioxide NPs (BTiO2 NPs) during pregnancy, the study aimed to evaluate the ability of an eco-friendly biosynthesis technique using garlic extract against maternal and fetal genotoxicities, which could result from repeated exposure to TiO2 NPs during gestation days (GD) 6–19. A total of fifty pregnant rats were divided into five groups (n = 10) and gavaged CTiO2 NPs and BTiO2 NPs at 100 and 300 mg/kg/day concentrations. Pregnant rats on GD 20 were anesthetized, uterine horns were removed, and then embryotoxicity was performed. The kidneys of the mothers and fetuses in each group were collected and then maintained in a frozen condition. Our results showed that garlic extract can be used as a reducing agent for the formation of TiO2 NPs. Moreover, BTiO2 NPs showed less toxic potential than CTiO2 NPs in HepG2 cells. Both chemically and biosynthesized TiO2 NP-induced genetic variation in the 16S rRNA sequences of mother groups compared to the control group. In conclusion, the genetic effects of the 16S rRNA sequence induced by chemically synthesized TiO2 NPs were greater than those of biosynthesized TiO2 NPs. However, there were no differences between the control group and the embryo-treated groups with chemically and biologically synthesized TiO2 NPs.
Fibroblast activation protein-α expression in fibroblasts is common in the tumor microenvironment of colorectal cancer and may serve as a therapeutic target
Authors: Greimelmaier K. , Klopp N. , Mairinger E. , Wessolly M. , Borchert S. , Steinborn J. , Schmid K. W. , Wohlschlaeger J. , Mairinger F. D.
Published: August 2023

Background: Colorectal cancer (CRC) is still one of the leading causes of cancer death worldwide, emphasizing the need for further diagnostic and therapeutic approaches. Cancer invasion and metastasis are affected by the tumor microenvironment (TME), with cancer-associated fibroblasts (CAF) being the predominant cellular component. An important marker for CAF is fibroblast activation protein-α (FAP) which has been evaluated as therapeutic target for, e.g., radioligand therapy. The aim of this study was to examine CRC regarding the FAP expression as a candidate for targeted therapy.

Methods: 67 CRC, 24 adenomas, 18 tissue samples of inflammation sites and 28 non-neoplastic, non-inflammatory tissue samples of colonic mucosa were evaluated for immunohistochemical FAP expression of CAF in tissue microarrays. The results were correlated with clinicopathological data, tumor biology and concurrent expression of additional immunohistochemical parameters.

Results: 53/67 (79%) CRC and 6/18 (33%) inflammatory tissue specimens showed expression of FAP. However, FAP was only present in 1/24 (4%) adenomas and absent in normal mucosa (0/28). Thus, FAP expression in CRC was significantly higher than in the other investigated groups. Within the CRC cohort, expression of FAP did not correlate with tumor stage, grading or the MSI status. However, it was observed that tumors exhibiting high immunohistochemical expression of Ki-67, CD3, p53, and β-Catenin showed a significantly higher incidence of FAP expression.

Conclusion: In the crosstalk between tumor cells and TME, CAF play a key role in carcinogenesis and metastatic spread. Expression of FAP was detectable in the majority of CRC but nearly absent in precursor lesions and non-neoplastic, non-inflammatory tissue. This finding indicates that FAP has the potential to emerge as a target for new diagnostic and therapeutic concepts in CRC. Additionally, the association between FAP expression and other immunohistochemical parameters displays the interaction between different components of the TME and demands further investigation.
Learning to Extrapolate Using Continued Fractions: Predicting the Critical Temperature of Superconductor Materials
Authors: Moscato Pablo , Haque Mohammad Nazmul , Huang Kevin , Sloan Julia , Corrales de Oliveira Jonathon
Published: August 2023

In the field of Artificial Intelligence (AI) and Machine Learning (ML), a common objective is the approximation of unknown target functions y=f(x) using limited instances S=(x(i),y(i)), where x(i)∈D and D represents the domain of interest. We refer to S as the training set and aim to identify a low-complexity mathematical model that can effectively approximate this target function for new instances x. Consequently, the model’s generalization ability is evaluated on a separate set T={x(j)}⊂D, where T≠S, frequently with T∩S=∅, to assess its performance beyond the training set. However, certain applications require accurate approximation not only within the original domain D but in an extended domain D′ that encompasses D as well. This becomes particularly relevant in scenarios involving the design of new structures, where minimizing errors in approximations is crucial. For example, when developing new materials through data-driven approaches, the AI/ML system can provide valuable insights to guide the design process by serving as a surrogate function. Consequently, the learned model can be employed to facilitate the design of new laboratory experiments. In this paper, we propose a method for multivariate regression based on iterative fitting of a continued fraction, incorporating additive spline models. We compare the performance of our method with established techniques, including AdaBoost, Kernel Ridge, Linear Regression, Lasso Lars, Linear Support Vector Regression, Multi-Layer Perceptrons, Random Forest, Stochastic Gradient Descent, and XGBoost. To evaluate these methods, we focus on an important problem in the field, namely, predicting the critical temperature of superconductors based on their physical–chemical characteristics.
The Influence of Coordinate Systems on the Stability Analysis of Lateral–Torsional Coupled Vibration
Authors: Qian Xin , Fan Yu , Wu Yaguang , Wang Wenjun , Li Lin
Published: August 2023

Stability analysis of lateral–torsional coupled vibration is obligatory for rotating machinery, such as aero-engines. However, the state-of-the-art method may lead to stability misjudgment under different coordinate systems. The cause of this misjudgment has not yet been well explored. The purpose of this paper is to clarify the error source of the stability analysis in a more comprehensive manner. A vertical Jeffcott rotor model including torsion vibration is built, and the Lagrange approach is applied to establish the motion equations. The coordinate transformation matrix is used to transfer the motion equations into the rotating coordinate system, making the coefficients of the motion equation constants. The differences in the unstable speed regions in the two coordinate systems are captured. The limitations of the Floquet theory and Hill’s determinant analysis in the stability estimation of the lateral–torsional coupled vibration are explained. It is found that, for Hill’s method, increasing the number of the harmonic truncation cannot correct the misjudgment, and the matrix truncation is the fundamental error source. The above research provides more accurate theoretical support for the analysis of the lateral–torsional coupling instability of rotors.
Archaeobotanical Insights into Kañawa (Chenopodium pallidicaule Aellen) Domestication: A Rustic Seed Crop of the Andean Altiplano
Authors: Bruno Maria C.
Published: August 2023

Kañawa/Cañihua (Chenopodium pallidicaule Aellen) is the lesser-known cousin of the domesticated Andean pseudocereal quinoa (Chenopodium quinoa Willd.). In 1970, Daniel Gade hypothesized that Andean farmers may have domesticated volunteer wild kañawa plants that occupied quinoa or potato fields after observing that they could survive harsh climatic events such as drought or frost. To revisit this question of kañawa domestication, this paper provides an overview of the current botanical, genetic, and archaeological knowledge of kañawa domestication. It then provides patterns in the presence of wild and domesticated kañawa seeds from archaeological sites in the southern Lake Titicaca Basin of Bolivia, spanning the Formative and Tiwanaku periods from approximately 1500 BCE to 1100 CE. This archaeobotanical evidence supports Gade’s hypothesis that kañawa was a later domesticate, not appearing until after 250 CE. Regional paleoclimatic evidence of frequent climatic fluctuations lends support to the argument that kañawa contributed to a diversified food supply, which could provide a buffer against climate risks.
Aerated Static Pile Composting for Industrial Biowastes: From Engineering to Microbiology
Authors: Keng Zi Xiang , Tan Jamie Jean Minn , Phoon Bao Lee , Khoo Chee Chang , Khoiroh Ianatul , Chong Siewhui , Supramaniam Christinavimala , Singh Ajit , Pan Guan-Ting
Published: August 2023

This work demonstrated the feasibility of an industrial-scale aerated static pile composting system for treating one of the common biowastes—soybean curd residue. The mixing ratios of the feedstock were optimized to achieve a carbon–nitrogen ratio and a moisture level in the ranges of 25–35 and 60–70%, respectively. This open-air composting system required 6–7 months to obtain a mature compost. Solvita and seed germination tests further confirmed the maturity of the compost, with 25% compost extract concentration yielding the best germination index in the absence of phytotoxicity. The bacterial and fungal compositions of the compost piles were further examined with metagenomic analysis. Thermoactinomyces spp., Oceanobacillus spp., and Kroppenstedtia spp. were among the unique bacteria found, and Diutina rugosa, Thermomyces dupontii, and Candida taylorii were among the unique fungi found in the compost piles, suggesting the presence of good microorganisms for degrading the organic biowastes.
Three-Dimensional Preoperative Planning Software for Hip Resurfacing Arthroplasty
Authors: Alagha M. Abdulhadi , Logishetty Kartik , O’Hanlon Ciaran , Liddle Alexander D. , Cobb Justin
Published: August 2023

Three-dimensional planning of hip arthroplasty is associated with better visualisation of anatomical landmarks and enhanced mapping for preoperative implant sizing, which can lead to a decrease in surgical time and complications. Despite the advantages of hip resurfacing arthroplasty (HRA), it is considered a technically challenging procedure and associated with inaccurate implant placement. This study aimed to examine the validity, reliability, and usability of preoperative 3D Hip Planner software for HRA. Fifty random cases of various hip osteoarthritis severity were planned twice by two junior trainees using the 3D Hip Planner within a one-month interval. Outcome measures included femoral/cup implant size, stem-shaft angle, and cup inclination angle, and were assessed by comparing outcomes from 2D and 3D planning. An adapted unified theory of acceptance and use of technology (UTAUT) survey was used for software usability. Bland–Altman plots between 3D and 2D planning for stem-shaft and inclination angles showed mean differences of 0.7 and −0.6, respectively (r = 0.93, p < 0.001). Stem-shaft and inclination angles showed inter-rater reliability biases of around −2° and 3°, respectively. Chi-square and Pearson’s correlation for femoral implant size showed a significant association between the two assessors (r = 0.91, p < 0.001). The 3D test–retest coefficient of repeatability for stem-shaft and inclination angles were around ±2° and ±3°, respectively, with a strong significant association for femoral implant size (r = 0.98, p < 0.001). Survey analyses showed that 70–90% agreed that 3D planning improved expectancy in four domains. 3D hip planner appears to be valid and reliable in preoperative HRA and shows significant potential in optimising the quality and accuracy of surgical planning.
Effects of a Subanesthetic Ketamine Infusion on Inflammatory and Behavioral Outcomes after Closed Head Injury in Rats
Authors: Spencer Haley F. , Boese Martin , Berman Rina Y. , Radford Kennett D. , Choi Kwang H.
Published: August 2023

Traumatic brain injury (TBI) affects millions of people annually, and most cases are classified as mild TBI (mTBI). Ketamine is a potent trauma analgesic and anesthetic with anti-inflammatory properties. However, ketamine’s effects on post-mTBI outcomes are not well characterized. For the current study, we used the Closed-Head Impact Model of Engineered Rotational Acceleration (CHIMERA), which replicates the biomechanics of a closed-head impact with resulting free head movement. Adult male Sprague–Dawley rats sustained a single-session, repeated-impacts CHIMERA injury. An hour after the injury, rats received an intravenous ketamine infusion (0, 10, or 20 mg/kg, 2 h period), during which locomotor activity was monitored. Catheter blood samples were collected at 1, 3, 5, and 24 h after the CHIMERA injury for plasma cytokine assays. Behavioral assays were conducted on post-injury days (PID) 1 to 4 and included rotarod, locomotor activity, acoustic startle reflex (ASR), and pre-pulse inhibition (PPI). Brain tissue samples were collected at PID 4 and processed for GFAP (astrocytes), Iba-1 (microglia), and silver staining (axonal injury). Ketamine dose-dependently altered locomotor activity during the infusion and reduced KC/GRO, TNF-α, and IL-1β levels after the infusion. CHIMERA produced a delayed deficit in rotarod performance (PID 3) and significant axonal damage in the optic tract (PID 4), without significant changes in other behavioral or histological measures. Notably, subanesthetic doses of intravenous ketamine infusion after mTBI did not produce adverse effects on behavioral outcomes in PID 1–4 or neuroinflammation on PID 4. A further study is warranted to thoroughly investigate beneficial effects of IV ketamine on mTBI given multi-modal properties of ketamine in traumatic injury and stress.
Cuproptosis- and m6A-Related lncRNAs for Prognosis of Hepatocellular Carcinoma
Authors: Zhu Yuezhi , Tan Jen Kit , Goon Jo Aan
Published: August 2023

Cuproptosis and N6-methyladenosine (m6A) have potential as prognostic predictors in cancer patients, but their roles in hepatocellular carcinoma (HCC) are unclear. This study aimed to screen a total of 375 HCC samples were retrieved from the TCGA database, and lncRNAs related to cuproptosis and m6A were obtained through correlation analysis. To construct a risk assessment model, univariate Cox regression analysis and LASSO Cox regression were employed. Analyze the regulatory effect of relevant risk assessment models on tumor mutation load (TMB) and immune microenvironment. A total of five lncRNAs (AC007405.3, AL031985.3, TMCC1-AS1, MIR210HG, TMEM220-AS1) with independent overall survival-related risk models were obtained by LASSO survival regression. TP53 and CTNNB1 were the three genes found to have the most mutations in high-risk group patients. The high-risk group with low TMB had the worst survival, whereas the low-risk group with high TMB had the best survival. KEGG pathway analysis revealed that the high-risk group was enriched with cell cycle, oocyte meiosis, cell senescence, and glycolysis/glucose production pathways. We constructed a reliable cuproptosis- and m6A-related lncRNA model for the prognosis of HCC. The model may provide new insights into managing HCC patients, but further research is needed to validate it.
The Mechanisms of Action of Hyperbaric Oxygen in Restoring Host Homeostasis during Sepsis
Authors: Vinkel Julie , Arenkiel Bjoern , Hyldegaard Ole
Published: August 2023

The perception of sepsis has shifted over time; however, it remains a leading cause of death worldwide. Sepsis is now recognized as an imbalance in host cellular functions triggered by the invading pathogens, both related to immune cells, endothelial function, glucose and oxygen metabolism, tissue repair and restoration. Many of these key mechanisms in sepsis are also targets of hyperbaric oxygen (HBO2) treatment. HBO2 treatment has been shown to improve survival in clinical studies on patients with necrotizing soft tissue infections as well as experimental sepsis models. High tissue oxygen tension during HBO2 treatment may affect oxidative phosphorylation in mitochondria. Oxygen is converted to energy, and, as a natural byproduct, reactive oxygen species are produced. Reactive oxygen species can act as mediators, and both these and the HBO2-mediated increase in oxygen supply have the potential to influence the cellular processes involved in sepsis. The pathophysiology of sepsis can be explained comprehensively through resistance and tolerance to infection. We argue that HBO2 treatment may protect the host from collateral tissue damage during resistance by reducing neutrophil extracellular traps, inhibiting neutrophil adhesion to vascular endothelium, reducing proinflammatory cytokines, and halting the Warburg effect, while also assisting the host in tolerance to infection by reducing iron-mediated injury and upregulating anti-inflammatory measures. Finally, we show how inflammation and oxygen-sensing pathways are connected on the cellular level in a self-reinforcing and detrimental manner in inflammatory conditions, and with support from a substantial body of studies from the literature, we conclude by demonstrating that HBO2 treatment can intervene to maintain homeostasis.
Telemedicine in Heart Failure in the COVID-19 and Post-Pandemic Era: What Have We Learned?
Authors: Sokolski Mateusz , Kalużna-Oleksy Marta , Tycińska Agnieszka , Jankowska Ewa A.
Published: August 2023

Numerous studies showed that patients with heart failure (HF) and COVID-19 are at high risk of in-hospital complications and long-term mortality. Changes in the organisation of the medical system during the pandemic also worsened access to standard procedures, increasing the general mortality in HF and forcing the systems to be reorganised with the implementation and development of telemedical technologies. The main challenges for HF patients during the pandemic could be solved with new technologies aimed to limit the risk of SARS-CoV-2 transmission, optimise and titrate the therapy, prevent the progression and worsening of HF, and monitor patients with acute HF events in the course of and after COVID-19. Dedicated platforms, phone calls or video conferencing and consultation, and remote non-invasive and invasive cardiac monitoring became potential tools used to meet the aforementioned challenges. These solutions showed to be effective in the model of care for patients with HF and undoubtedly will be developed after the experience of the pandemic. However, the multitude of possibilities requires central coordination and collaboration between institutes with data protection and cost reimbursement to create effective mechanisms in HF management. It is crucial that lessons be learned from the pandemic experience to improve the quality of care for HF patients.
Bioinspired Perception and Navigation of Service Robots in Indoor Environments: A Review
Authors: Wang Jianguo , Lin Shiwei , Liu Ang
Published: August 2023

Biological principles draw attention to service robotics because of similar concepts when robots operate various tasks. Bioinspired perception is significant for robotic perception, which is inspired by animals’ awareness of the environment. This paper reviews the bioinspired perception and navigation of service robots in indoor environments, which are popular applications of civilian robotics. The navigation approaches are classified by perception type, including vision-based, remote sensing, tactile sensor, olfactory, sound-based, inertial, and multimodal navigation. The trend of state-of-art techniques is moving towards multimodal navigation to combine several approaches. The challenges in indoor navigation focus on precise localization and dynamic and complex environments with moving objects and people.
A Novel Artificial-Intelligence-Based Approach for Classification of Parkinson’s Disease Using Complex and Large Vocal Features
Authors: Nijhawan Rahul , Kumar Mukul , Arya Sahitya , Mendirtta Neha , Kumar Sunil , Towfek S. K. , Khafaga Doaa Sami , Alkahtani Hend K. , Abdelhamid Abdelaziz A.
Published: August 2023

Parkinson’s disease (PD) affects a large proportion of elderly people. Symptoms include tremors, slow movement, rigid muscles, and trouble speaking. With the aging of the developed world’s population, this number is expected to rise. The early detection of PD and avoiding its severe consequences require a precise and efficient system. Our goal is to create an accurate AI model that can identify PD using human voices. We developed a transformer-based method for detecting PD by retrieving dysphonia measures from a subject’s voice recording. It is uncommon to use a neural network (NN)-based solution for tabular vocal characteristics, but it has several advantages over a tree-based approach, including compatibility with continuous learning and the network’s potential to be linked with an image/voice encoder for a more accurate multi modal solution, shifting SOTA approach from tree-based to a neural network (NN) is crucial for advancing research in multimodal solutions. Our method outperforms the state of the art (SOTA), namely Gradient-Boosted Decision Trees (GBDTs), by at least 1% AUC, and the precision and recall scores are also improved. We additionally offered an XgBoost-based feature-selection method and a fully connected NN layer technique for including continuous dysphonia measures, in addition to the solution network. We also discussed numerous important discoveries relating to our suggested solution and deep learning (DL) and its application to dysphonia measures, such as how a transformer-based network is more resilient to increased depth compared to a simple MLP network. The performance of the proposed approach and conventional machine learning techniques such as MLP, SVM, and Random Forest (RF) have also been compared. A detailed performance comparison matrix has been added to this article, along with the proposed solution’s space and time complexity.
Power Benefits of High-Altitude Flapping Wing Flight at the Monarch Butterfly Scale
Authors: Kang Chang-kwon , Sridhar Madhu , Twigg Rachel , Pohly Jeremy , Lee Taeyoung , Aono Hikaru
Published: August 2023

The long-range migration of monarch butterflies, extended over 4000 km, is not well understood. Monarchs experience varying density conditions during migration, ranging as high as 3000 m, where the air density is much lower than at sea level. In this study, we test the hypothesis that the aerodynamic performance of monarchs improves at reduced density conditions by considering the fluid–structure interaction of chordwise flexible wings. A well-validated, fully coupled Navier–Stokes/structural dynamics solver was used to illustrate the interplay between wing motion, aerodynamics, and structural flexibility in forward flight. The wing density and elastic modulus were measured from real monarch wings and prescribed as inputs to the aeroelastic framework. Our results show that sufficient lift is generated to offset the butterfly weight at higher altitudes, aided by the wake-capture mechanism, which is a nonlinear wing–wake interaction mechanism, commonly seen for hovering animals. The mean total power, defined as the sum of the aerodynamic and inertial power, decreased by 36% from the sea level to the condition at 3000 m. Decreasing power with altitude, while maintaining the same equilibrium lift, suggests that the butterflies generate lift more efficiently at higher altitudes.
The Role of Swelling in the Regulation of OPA1-Mediated Mitochondrial Function in the Heart In Vitro
Authors: Chapa-Dubocq Xavier R. , Rodríguez-Graciani Keishla M. , García-Báez Jorge , Vadovsky Alyssa , Bazil Jason N. , Javadov Sabzali
Published: August 2023

Optic atrophy-1 (OPA1) plays a crucial role in the regulation of mitochondria fusion and participates in maintaining the structural integrity of mitochondrial cristae. Here we elucidate the role of OPA1 cleavage induced by calcium swelling in the presence of Myls22 (an OPA1 GTPase activity inhibitor) and TPEN (an OMA1 inhibitor). The rate of ADP-stimulated respiration was found diminished by both inhibitors, and they did not prevent Ca2+-induced mitochondrial respiratory dysfunction, membrane depolarization, or swelling. L-OPA1 cleavage was stimulated at state 3 respiration; therefore, our data suggest that L-OPA1 cleavage produces S-OPA1 to maintain mitochondrial bioenergetics in response to stress.
Biotechnological Potential of the Stress Response and Plant Cell Death Regulators Proteins in the Biofuel Industry
Authors: Bernacki Maciej Jerzy , Mielecki Jakub , Antczak Andrzej , Drożdżek Michał , Witoń Damian , Dąbrowska-Bronk Joanna , Gawroński Piotr , Burdiak Paweł , Marchwicka Monika , Rusaczonek Anna , Dąbkowska-Susfał Katarzyna , Strobel Wacław Roman , Mellerowicz Ewa J. , Zawadzki Janusz , Szechyńska-Hebda Magdalena , Karpiński Stanisław
Published: August 2023

Production of biofuel from lignocellulosic biomass is relatively low due to the limited knowledge about natural cell wall loosening and cellulolytic processes in plants. Industrial separation of cellulose fiber mass from lignin, its saccharification and alcoholic fermentation is still cost-ineffective and environmentally unfriendly. Assuming that the green transformation is inevitable and that new sources of raw materials for biofuels are needed, we decided to study cell death—a natural process occurring in plants in the context of reducing the recalcitrance of lignocellulose for the production of second-generation bioethanol. “Members of the enzyme families responsible for lysigenous aerenchyma formation were identified during the root hypoxia stress in Arabidopsis thaliana cell death mutants. The cell death regulatory genes, LESION SIMULATING DISEASE 1 (LSD1), PHYTOALEXIN DEFICIENT 4 (PAD4) and ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) conditionally regulate the cell wall when suppressed in transgenic aspen. During four years of growth in the field, the following effects were observed: lignin content was reduced, the cellulose fiber polymerization degree increased and the growth itself was unaffected. The wood of transgenic trees was more efficient as a substrate for saccharification, alcoholic fermentation and bioethanol production. The presented results may trigger the development of novel biotechnologies in the biofuel industry.
Human Blood Serum Can Diminish EGFR-Targeted Inhibition of Squamous Carcinoma Cell Growth through Reactivation of MAPK and EGFR Pathways
Authors: Kamashev Dmitri , Shaban Nina , Lebedev Timofey , Prassolov Vladimir , Suntsova Maria , Raevskiy Mikhail , Gaifullin Nurshat , Sekacheva Marina , Garazha Andrew , Poddubskaya Elena , Sorokin Maksim , Buzdin Anton
Published: August 2023

Regardless of the presence or absence of specific diagnostic mutations, many cancer patients fail to respond to EGFR-targeted therapeutics, and a personalized approach is needed to identify putative (non)responders. We found previously that human peripheral blood and EGF can modulate the activities of EGFR-specific drugs on inhibiting clonogenity in model EGFR-positive A431 squamous carcinoma cells. Here, we report that human serum can dramatically abolish the cell growth rate inhibition by EGFR-specific drugs cetuximab and erlotinib. We show that this phenomenon is linked with derepression of drug-induced G1S cell cycle transition arrest. Furthermore, A431 cell growth inhibition by cetuximab, erlotinib, and EGF correlates with a decreased activity of ERK1/2 proteins. In turn, the EGF- and human serum-mediated rescue of drug-treated A431 cells restores ERK1/2 activity in functional tests. RNA sequencing revealed 1271 and 1566 differentially expressed genes (DEGs) in the presence of cetuximab and erlotinib, respectively. Erlotinib- and cetuximab-specific DEGs significantly overlapped. Interestingly, the expression of 100% and 75% of these DEGs restores to the no-drug level when EGF or a mixed human serum sample, respectively, is added along with cetuximab. In the case of erlotinib, EGF and human serum restore the expression of 39% and 83% of DEGs, respectively. We further assessed differential molecular pathway activation levels and propose that EGF/human serum-mediated A431 resistance to EGFR drugs can be largely explained by reactivation of the MAPK signaling cascade.
Work Gains and Strains on Father Involvement: The Mediating Role of Parenting Styles
Authors: Diniz Eva , Monteiro Lígia , Veríssimo Manuela
Published: August 2023

The balance between work and family demands is one of the main challenges of contemporary parenting. However, most of the research has focused on mothers’ perspectives, with fathers’ perspectives about the links between work–family activities and father involvement, as well as the role of indirect effects, such as parenting styles, being less explored. This study aims to bridge these gaps by exploring whether work strains or gains are related to father involvement in childcare and the mediating role of parental styles, focusing on fathers’ reports. Working, married fathers of preschoolers (n = 411) self-reported about work strains and gains, parental styles, and father involvement. Structural equation modeling, using maximum-likelihood estimation, provided good fit indices. Results of the bootstrap analysis revealed how fathers’ gains indirectly increased involvement both in direct and indirect care through positive parental styles. Otherwise, fathers’ strains at work had a negative indirect effect on direct care through negative parental styles. Findings contribute to work–family interface by showing how parental styles account for mediating environmental challenges on father involvement.