bluuebunny/crossref_metadata_2025 huggingface dataset:
A COMPREHENSIVE ANALYSIS OF FOUR CHANNEL CWDM NETWORK SYSTEM
Authors: Koduru ManojKumar , B.D Venkatramana Reddy
Published: July 2023

Growth of optical fiber communication has led to an increased demand for high bandwidth and long-distance transmission capabilities. In response, telecommunication companies are using DWDM network system is able to satisfy the demand, though this approach is often costly for customers. As an alternative, the CWDM network system has been proposed to provide a lower cost solution for high bandwidth transmission.
The proposed study evaluates the effectiveness of transmitting various bandwidths for each channel in a 4-channel CWDM system using Opti System software. The study assesses the system's performance by measuring the extinction ratio and the separation of transmitter and receiver up to a fiber span of 100 km. This research focuses on applications that require shorter distances and where cost is a critical factor.
The outcomes of this research can be used to improve the design and functionality of CWDM systems by illuminating their strengths and weaknesses. The usage of CWDM technology offers a cost-effective solution for telecommunication companies to meet the increasing demand for high bandwidth transmission while also providing a lower cost option for customers.
Absence of E-Cadherin and β-Catenin in the Basal Plasma Membrane of Collecting Duct Cells During NDI Development and Recovery
Authors: Sørtvedt Xabier , Nielsen Rikke , Praetorius Jeppe , Christensen Birgitte M.
Published: July 2023

Lithium (Li) induces severe polyuria and polydipsia in up to 40% of patients undergoing Li treatment. In rats, Li treatment induces a reversible cellular remodeling of the collecting duct (CD), decreasing the fraction of principal-to-intercalated cells. To investigate the potential role of adherens junction proteins, we performed immunohistochemistry on kidney cross-sections from rats treated with Li as well as rats undergoing recovery on a normal diet following 4 weeks of Li-treatment. We performed immunoelectron microscopy on cryosections to determine the ultrastructural localizations. Immunohistochemistry showed that E-cadherin and β-catenin were present in both the lateral and basal plasma membrane domains of CD cells. Immunoelectron microscopy confirmed that β-catenin was localized both to the lateral and the basal plasma membrane. The basal localization of both proteins was absent from a fraction of mainly principal cells after 10 and 15 days of Li-treatment. After 4 weeks of Li-treatment few to no cells were absent of E-cadherin and β-catenin at the basal plasma membrane. After 12 and 19 days of recovery some cells exhibited an absence of basal localization of both proteins. Thus, the observed localizational changes of E-cadherin and β-catenin appear before the cellular remodeling during both development and recovery from Li-NDI:
Diamonds in the not-so-rough: Wild relative diversity hidden in crop genomes
Authors: Flint-Garcia Sherry , Feldmann Mitchell J. , Dempewolf Hannes , Morrell Peter L. , Ross-Ibarra Jeffrey
Published: July 2023

Crop production is becoming an increasing challenge as the global population grows and the climate changes. Modern cultivated crop species are selected for productivity under optimal growth environments and have often lost genetic variants that could allow them to adapt to diverse, and now rapidly changing, environments. These genetic variants are often present in their closest wild relatives, but so are less desirable traits. How to preserve and effectively utilize the rich genetic resources that crop wild relatives offer while avoiding detrimental variants and maladaptive genetic contributions is a central challenge for ongoing crop improvement. This Essay explores this challenge and potential paths that could lead to a solution.
Loss of STING in parkin mutant flies suppresses muscle defects and mitochondria damage
Authors: Moehlman Andrew T. , Kanfer Gil , Youle Richard J.
Published: July 2023

The early pathogenesis and underlying molecular causes of motor neuron degeneration in Parkinson’s Disease (PD) remains unresolved. In the model organism Drosophila melanogaster, loss of the early-onset PD gene parkin (the ortholog of human PRKN) results in impaired climbing ability, damage to the indirect flight muscles, and mitochondrial fragmentation with swelling. These stressed mitochondria have been proposed to activate innate immune pathways through release of damage associated molecular patterns (DAMPs). Parkin-mediated mitophagy is hypothesized to suppress mitochondrial damage and subsequent activation of the cGAS/STING innate immunity pathway, but the relevance of this interaction in the fly remains unresolved. Using a combination of genetics, immunoassays, and RNA sequencing, we investigated a potential role for STING in the onset of parkin-null phenotypes. Our findings demonstrate that loss of Drosophila STING in flies rescues the thorax muscle defects and the climbing ability of parkin-/- mutants. Loss of STING also suppresses the disrupted mitochondrial morphology in parkin-/- flight muscles, suggesting unexpected feedback of STING on mitochondria integrity or activation of a compensatory mitochondrial pathway. In the animals lacking both parkin and sting, PINK1 is activated and cell death pathways are suppressed. These findings support a unique, non-canonical role for Drosophila STING in the cellular and organismal response to mitochondria stress.
Factors associated with knowledge and use of home pregnancy test kits in Uganda
Authors: Kamei Akito , Sato Ryoko , Thornton Rebecca
Published: July 2023

Early detection of pregnancy status may help women initiate earlier antenatal care and healthy pregnancy behaviors, which could lead to healthier mothers and infants. Pregnancy tests are inexpensive and easy to use; meanwhile, little attention has been given to understanding women’s knowledge and use of home pregnancy tests, especially in developing countries. We analyze cross-sectional data collected from 1,008 women ages 18–35, living in Northern Uganda in 2019, who are most likely to be uncertain about their pregnancy status. The survey asked women if they had knowledge of or had ever used a home pregnancy test kit, and barriers to purchasing a home pregnancy test kit. Among the 1,008 women, 65 percent report knowledge of home pregnancy test kits, and 29 percent report having ever used a test kit. Women who have heard of pregnancy test kits have higher levels of education, are in higher wealth quintiles, are more likely to have a salaried occupation and live closer to a health facility. Among women who report knowledge of home pregnancy test kits (N = 657), 90 percent report needing to ask their husband or partner for money to purchase a test kit, seven percent report they would hide the purchase, and 31 percent report that their husband or partner would not support the purchase. Women who report a lack of support from their husband or partner tend to be older, are more likely to have had prior pregnancies, are less likely to have a salaried occupation, are less likely to want a/another child, and are more likely to have a husband or partner desiring more children than herself. Future research aimed at understanding how and whether these barriers affect the utilization of home pregnancy test kits could help inform policymakers on how to increase the use of home pregnancy test kits.

Trial registration: NCT03975933. Registered 05 June 2019, https://clinicaltrials.gov/ct2/show/record/NCT03975933.
Characterization of Latin American migrants at risk for Trypanosoma cruzi infection in a non-endemic setting. Insights into initial evaluation of cardiac and digestive involvement
Authors: Laynez-Roldán Pedro , Losada-Galván Irene , Posada Elizabeth , de la Torre Ávila Leonardo , Casellas Aina , Sanz Sergi , Subirà Carme , Rodriguez-Valero Natalia , Camprubí-Ferrer Daniel , Vera Isabel , Roldán Montserrat , Aldasoro Edelweiss , Oliveira-Souto Inés , Calvo-Cano Antonia , Valls Maria-Eugenia , Álvarez-Martínez Míriam J. , Gállego Montserrat , Abras Alba , Ballart Cristina , Muñoz José , Gascón Joaquim , Pinazo Maria-Jesus
Published: July 2023

Background

Trypanosoma cruzi causes Chagas disease (CD), a potentially fatal disease characterized by cardiac disorders and digestive, neurological or mixed alterations. T. cruzi is transmitted to humans by the bite of triatomine vectors; both the parasite and disease are endemic in Latin America and the United States. In the last decades, population migration has changed the classic epidemiology of T. cruzi, contributing to its global spread to traditionally non-endemic countries. Screening is recommended for Latin American populations residing in non-endemic countries.

Methods

The present study analyzes the epidemiological characteristics of 2,820 Latin American individuals who attended the International Health Service (IHS) of the Hospital Clinic de Barcelona between 2002 and 2019. The initial assessment of organ damage among positive cases of T. cruzi infection was analyzed, including the results of electrocardiogram (ECG), echocardiogram, barium enema and esophagogram.

Results

Among all the screened individuals attending the clinic, 2,441 (86.6%) were born in Bolivia and 1,993 (70.7%) were female. Of individuals, 1,517 (81.5%) reported previous exposure to the vector, which is a strong risk factor associated with T. cruzi infection; 1,382 individuals were positive for T. cruzi infection. The first evaluation of individuals with confirmed T. cruzi infection, showed 148 (17.1%) individuals with Chagasic cardiomyopathy, the main diagnostic method being an ECG and the right bundle branch block (RBBB) for the most frequent disorder; 16 (10.8%) individuals had a normal ECG and were diagnosed of Chagasic cardiomyopathy by echocardiogram.

Conclusions

We still observe many Latin American individuals who were at risk of T. cruzi infection in highly endemic areas in their countries of origin, and who have not been previously tested for T. cruzi infection. In fact, even in Spain, a country with one of the highest proportion of diagnosis of Latin American populations, T. cruzi infection remains underdiagnosed. The screening of Latin American populations presenting with a similar profile as reported here should be promoted. ECG is considered necessary to assess Chagasic cardiomyopathy in positive individuals, but echocardiograms should also be considered as a diagnostic approach given that it can detect cardiac abnormalities when the ECG is normal.
A systematic review on maternal-to-infant transfer of drugs through breast milk during the treatment of malaria, tuberculosis, and neglected tropical diseases
Authors: Ojara Francis Williams , Kawuma Aida N. , Waitt Catriona
Published: July 2023

Background

Exclusive breastfeeding of infants under 6 months of age is recommended by the World Health Organization. In 2021, over 300 million combined incident cases of malaria, tuberculosis, and neglected tropical diseases (NTDs) were reported, predominantly in low-income countries. For many of the drugs used as first-line treatments for these conditions, there is limited knowledge on infant exposure through breastfeeding with poorly understood consequences. This review summarized available knowledge on mother-to-infant transfer of these drugs to inform future lactation pharmacokinetic studies.

Methodology

A list of first-line drugs was generated from the latest WHO treatment guidelines. Using standard online databases, 2 independent reviewers searched for eligible articles reporting lactation pharmacokinetics studies and extracted information on study design, participant characteristics, and the mathematical approach used for parameter estimation. A third reviewer settled any disagreements between the 2 reviewers. All studies were scored against the standardized “ClinPK” checklist for conformity to best practices for reporting clinical pharmacokinetic studies. Simple proportions were used to summarize different study characteristics.

Findings

The most remarkable finding was the scarcity of lactation pharmacokinetic data. Only 15 of the 69 drugs we listed had lactation pharmacokinetics fully characterized. Most studies enrolled few mothers, and only one evaluated infant drug concentrations. Up to 66% of the studies used non-compartmental analysis to estimate pharmacokinetic parameters rather than model-based compartmental analysis. Unlike non-compartmental approaches, model-based compartmental analysis provides for dynamic characterization of individual plasma and breast milk concentration-time profiles and adequately characterizes variability within and between individuals, using sparsely sampled data. The “ClinPK” checklist inadequately appraised the studies with variability in the number of relevant criteria across different studies.

Conclusions/significance

A consensus is required on best practices for conducting and reporting lactation pharmacokinetic studies, especially in neglected diseases such as malaria, tuberculosis, and NTDs, to optimize treatment of mother–infant pairs.
The Aedes aegypti RNA interference response against Zika virus in the context of co-infection with dengue and chikungunya viruses
Authors: Leggewie Mayke , Scherer Christina , Altinli Mine , Gestuveo Rommel J. , Sreenu Vattipally B. , Fuss Janina , Vazeille Marie , Mousson Laurence , Badusche Marlis , Kohl Alain , Failloux Anna-Bella , Schnettler Esther
Published: July 2023

Since its detection in 2015 in Brazil, Zika virus (ZIKV) has remained in the spotlight of international public health and research as an emerging arboviral pathogen. In addition to single infection, ZIKV may occur in co-infection with dengue (DENV) and chikungunya (CHIKV) viruses, with whom ZIKV shares geographic distribution and the mosquito Aedes aegypti as a vector. The main mosquito immune response against arboviruses is RNA interference (RNAi). It is unknown whether or not the dynamics of the RNAi response differ between single arboviral infections and co-infections. In this study, we investigated the interaction of ZIKV and DENV, as well as ZIKV and CHIKV co-infections with the RNAi response in Ae. aegypti. Using small RNA sequencing, we found that the efficiency of small RNA production against ZIKV -a hallmark of antiviral RNAi—was mostly similar when comparing single and co-infections with either DENV or CHIKV. Silencing of key antiviral RNAi proteins, showed no change in effect on ZIKV replication when the cell is co-infected with ZIKV and DENV or CHIKV. Interestingly, we observed a negative effect on ZIKV replication during CHIKV co-infection in the context of Ago2-knockout cells, though his effect was absent during DENV co-infection. Overall, this study provides evidence that ZIKV single or co-infections with CHIKV or DENV are equally controlled by RNAi responses. Thus, Ae. aegypti mosquitoes and derived cells support co-infections of ZIKV with either CHIKV or DENV to a similar level than single infections, as long as the RNAi response is functional.
Snakebite envenomations and access to treatment in communities of two indigenous areas of the Western Brazilian Amazon: A cross-sectional study
Authors: de Farias Altair Seabra , Gomes Filho Manoel Rodrigues , da Costa Arévalo Macio , Cristino Joseir Saturnino , Farias Franciane Ribeiro , Sachett André , Silva-Neto Alexandre Vilhena , de Carvalho Fabíola Guimarães , Ambrosio Sediel Andrade , da Silva Carvalho Erica , Lacerda Marcus , Murta Felipe , Machado Vinícius Azevedo , Wen Fan Hui , Monteiro Wuelton , Sachett Jacqueline
Published: July 2023

Background

The indigenous populations of Brazil present poor health indicators and a disproportionate prevalence and case-fatality rate of neglected tropical diseases, including snakebite envenomations (SBEs). This study aims to estimate access to medical care for SBEs and analyze the barriers that prevent victims from accessing healthcare in indigenous communities in two health districts located in the Western Brazilian Amazon.

Methodology/Principal findings

This cross-sectional study used semi-structured interviews to collect data from individuals who experienced SBEs in the Upper Rio Solimões and Upper Rio Negro indigenous health districts. Of the 187 participants, 164 (87.7%) reported that they had access to healthcare and received assistance in a hospital in the urban area of the municipalities. Frequency was 95.4% in the Upper Rio Solimões SIHD, and 69.6% in the Upper Rio Negro SIHD (P<0.0001). The study found that the availability of indigenous medicine as the only choice in the village was the main reason for not accessing healthcare (75%), followed by a lack of financial resources and means of transportation (28.1%). Four deaths were reported from SBEs, resulting in a case-fatality rate of 2.1%.

Conclusions/Significance

In the study areas, there are records of SBE patients who did not receive medical attention. Availability of pre-hospital emergency transport using motorboats, a greater number of hospitals and better navigability of the Solimões River and its tributaries would make access easier for indigenous people living in the region of the Upper Solimões River. The implementation of cross-cultural hospital care needs to be considered in order to reduce the resistance of indigenous populations in relation to seeking treatment for SBEs.
Multifaceted Empathy Test (MET): Validity evidence for the Brazilian population concerning the computer-based (face-to-face) and online versions
Authors: Fernandes Vieira de Lima Felipe , Rossi Giordano , dos Santos Rafael Guimarães , de Lima Osório Flávia
Published: July 2023

Background

The lack of empathy is associated with several psychological and behavioral disorders, and it is important to assess this construct broadly, through multi-methods.

Objective

To conduct a psychometric analysis of the Brazilian version of the Multifaceted Empathy Test (MET), a computerized task that assesses emotional and cognitive empathy.

Methods

The samples were recruited from the community using the snowball method (phase 1: face-to-face; N = 142) and through social media (phase 2: online; N = 519). The participants completed the MET and the Interpersonal Reactivity Index (IRI) to assess the convergent validity between the instruments. To assess validity with correlated constructs (resilient coping and stress), the Brief Resilient Coping Scale and Perceived Stress Scale were used. A task was also implemented in the face-to-face application to assess facial emotions. The retest was applied 25 days later to a portion of the sample (face-to-face: N = 31; online: N = 102).

Results

It was observed adequate test-retest reliability for most items (ICC = 0.49–0.98), satisfactory infit and outfit indexes, discriminatory ability between sexes, weak convergent validity with empathy measures (r = 0.17–0.36), and correlate constructs (r = 0.12–0.46). MET presented good psychometric indicators, confirming its use in face-to-face/computer-based and online formats in clinical and research contexts. However, weaknesses were found regarding the cognitive subscale, demanding future studies to address larger samples to enable more robust conclusions concerning its adequacy. Further research on the instrument’s internal structure can also contribute to its improvement.
On closing the inopportune gap with consistency transformation and iterative refinement
Authors: João Mario , Sena Alexandre C. , Rebello Vinod E. F.
Published: July 2023

The problem of aligning multiple biological sequences has fascinated scientists for a long time. Over the last four decades, tens of heuristic-based Multiple Sequence Alignment (MSA) tools have been proposed, the vast majority being built on the concept of Progressive Alignment. It is known, however, that this approach suffers from an inherent drawback regarding the inadvertent insertion of gaps when aligning sequences. Two well-known corrective solutions have frequently been adopted to help mitigate this: Consistency Transformation and Iterative Refinement. This paper takes a tool-independent technique-oriented look at the alignment quality benefits of these two strategies using problem instances from the HOMSTRAD and BAliBASE benchmarks. Eighty MSA aligners have been used to compare 4 classes of heuristics: Progressive Alignments, Iterative Alignments, Consistency-based Alignments, and Consistency-based Progressive Alignments with Iterative Refinement. Statistically, while both Consistency-based classes are better for alignments with low similarity, for sequences with higher similarity, the differences between the classes are less clear. Iterative Refinement has its own drawbacks resulting in there being statistically little advantage for Progressive Aligners to adopt this technique either with Consistency Transformation or without. Nevertheless, all 4 classes are capable of bettering each other, depending on the instance problem. This further motivates the development of MSA frameworks, such as the one being developed for this research, which simultaneously contemplate multiple classes and techniques in their attempt to uncover better solutions.
Can static Rorschach stimuli perceived as in motion affect corticospinal excitability?
Authors: Andò Agata , Garbarini Francesca , Giromini Luciano , Salatino Adriana , Zennaro Alessandro , Ricci Raffaella , Fossataro Carlotta
Published: July 2023

It has been proposed that seeing human movement or activity (M), while trying to say what the static Rorschach inkblot design look like, is accompanied by Mirror Neuron System (MNS)-like mirroring activity in the brain. The present study aimed to investigate whether the Rorschach cards eliciting M responses could affect the excitability of the motor cortex by recording motor evoked potentials (MEPs) elicited by single-pulse TMS over the primary motor cortex (M1). We hypothesized that Rorschach inkblot stimuli triggering the viewer’s experience of human movement would increase corticospinal excitability. Twenty-one healthy volunteers (15 women) participated in the preliminary experiment, while another different sample of twenty-two healthy participants (11 women) ranging in age from 21 to 41 years was enrolled in the main experiment. Our results showed that the Rorschach cards known to be associated with a high number of M responses elicited human movement both as automatic internal sensations and as verbal production of responses involving human movement. However, contrary to our hypothesis, the reported internal feeling of human movement had no corresponding physiological counterpart, as the amplitude of MEPs did not increase. Possible and innovative explanations for the involvement of bottom-up and top-down processes were provided.
Using real-time ascertainment rate estimate from infection and hospitalization dataset for modeling the spread of infectious disease: COVID-19 case study in the Czech Republic
Authors: Přibylová Lenka , Eclerová Veronika , Májek Ondřej , Jarkovský Jiří , Pavlík Tomáš , Dušek Ladislav
Published: July 2023

We present a novel approach to estimate the time-varying ascertainment rate in almost real-time, based on the surveillance of positively tested infectious and hospital admission data. We also address the age dependence of the estimate. The ascertainment rate estimation is based on the Bayes theorem. It can be easily calculated and used (i) as part of a mechanistic model of the disease spread or (ii) to estimate the unreported infections or changes in their proportion in almost real-time as one of the early-warning signals in case of undetected outbreak emergence. The paper also contains a case study of the COVID-19 epidemic in the Czech Republic. The case study demonstrates the usage of the ascertainment rate estimate in retrospective analysis, epidemic monitoring, explanations of differences between waves, usage in the national Anti-epidemic system, and monitoring of the effectiveness of non-pharmaceutical interventions on Czech nationwide surveillance datasets. The Czech data reveal that the probability of hospitalization due to SARS-CoV-2 infection for the senior population was 12 times higher than for the non-senior population in the monitored period from the beginning of March 2020 to the end of May 2021. In a mechanistic model of COVID-19 spread in the Czech Republic, the ascertainment rate enables us to explain the links between all basic compartments, including new cases, hospitalizations, and deaths.
Multidisciplinary analyses on the 11th-12th century bronze doors of San Marco, Venice
Authors: Mödlinger Marianne , Bernabei Mauro , Bontadi Jarno , Fellin Marco , Fera Martin , Ghiara Giorgia , Negri Martino , Utz Judith
Published: July 2023

Two 11th- and 12th-century entrance doors from the Basilica di San Marco in Venice, made of different copper alloys and woods, were non-invasively examined in situ. The chemical composition of the metals, the way in which different metal parts were joined together, the tree species used to construct the supporting structures and the age of the wood are determined. A portable ED-XRF instrument and optical microscopes were used. The doors were also photographed to produce high-resolution orthophotos and 3D models. The metal parts of the doors were made of leaded tin-bronze and quaternary Cu-Sn-Zn-Pb alloys and were mounted on a wooden multi-layer structure of larch and silver fir; the dendrochronological dates of some of the larch boards are 1965, teminus post quem.
How do housing prices affect innovation and entrepreneurship? Evidence from China
Authors: Fan Jianshuang , Liu Dongtao , Hu Mingzhi , Zang Yipeng
Published: July 2023

This paper analyzes how housing prices affect innovation and entrepreneurship. We construct a city-level panel dataset including 281 cities between 2009 and 2019 by merging housing price data from China Statistical Yearbook for Regional Economy with innovation and entrepreneurship data from Peking University Open Research Data Platform. Our results suggest that housing prices are positively associated with the vitality of innovation and entrepreneurship (VIE). The results remain consistent with a series of robustness checks. We also find that rising house prices promote VIE through the wealth effect and the siphon effect. Spatial effect analysis further shows that housing prices not only positively affect the VIE of local cities, but also positively affect the VIE of neighboring cities. These findings imply the necessity of curbing the excessive rise of housing prices and decoupling public services and benefits related to homeownership.
Klebsiella pneumoniae carbapenamases in Escherichia coli isolated from humans and livestock in rural south-western Uganda
Authors: Tuhamize Barbra , Asiimwe Benon B. , Kasaza Kennedy , Sabiiti Wilber , Holden Mathew , Bazira Joel
Published: July 2023

Background

The accumulation of resistance genes in Escherichia coli (E. coli) strains imposes limitations in the therapeutic options available for the treatment of infections caused by E.coli. Production of Klebsiella pneumoniae carbapenemase (KPC) by E. coli renders it resistant to broad-spectrum β-lactam antibiotics. Globally there is existing evidence of spread of carbapenem-resistant E. coli in both humans and livestock driven by acquisition of the several other carbapenemase genes. Overall, there is little information regarding the extent of KPC gene distribution in E. coli. We set out to determine the prevalence, and evaluate the phenotypic and genotypic patterns of KPC in E. coli isolated from humans and their livestock in rural south western Uganda.

Methods

A laboratory-based, descriptive cross-sectional study was conducted involving 96 human and 96 livestock isolates collected from agro-pastoralist communities in Mbarara district in south western Uganda. Phenotypic and molecular methods (PCR) were used for presence and identification of KPC genes in the E. coli isolates. A chi-square test of independence was used to evaluate the differences in resistant patterns between carbapenems and isolates.

Results

The overall prevalence of carbapenem resistance by disk diffusion susceptibility testing (DST) for both humans and livestock isolates were 41.7% (80/192). DST-based resistance was identical in both human and livestock isolates (41.7%). The prevalence of carbapenem resistance based on Modified Hodge Test (MHT) was 5% (2/40) and 10% (4/40) for humans and livestock isolates respectively. Both human and livestock isolates, 48.7% (95/192) had the KPC gene, higher than phenotypic expression; 41.7% (80/192). blaKPC gene prevalence was overall similar in human isolates (51%; 49/96) vs livestock isolates (47.9%; 46/96). Approximately, 19% (15/80) of the isolates were phenotypically resistant to carbapenems and over 70% (79/112) of the phenotypically sensitive strains harbored the blaKPC gene.

Conclusion

Our results suggest that both human and livestock isolates of E. coli in our setting carry the blaKPC gene with a high percentage of strains not actively expressing the blaKPC gene. The finding of fewer isolates carrying the KPC gene than those phenotypically resistant to carbapenems suggests that other mechanisms are playing a role in this phenomenon, calling for further researcher into this phenomenon.
The role of scenic context on upright face preference in infancy
Authors: Kobayashi Megumi , Kanazawa So , Yamaguchi Masami K.
Published: July 2023

Scenic information plays an important role in face processing, whereas it has received limited attention in the field of developmental research. In the current study, we investigated whether infants, like adults, utilize scenic information for face processing by the preferential-looking method. In Experiment 1, we examined 4–5 and 6-7-month-olds’ visual preferences for upright faces compared to inverted faces in two surrounding scene conditions: intact (in which a face occurs in an intact scene) and scrambled (in which a face occurs in a jumbled scene). We found that 6- to 7-month-olds preferred the upright face in the intact scene, but not in the scrambled scene. Meanwhile, 4- to 5-month-olds showed significant upright face preference in both scenes. The results of Experiment 2 ruled out the possibility that the lack of preference for upright faces in the scrambled scene in 6- to 7-month-olds resulted from more distraction by the scrambledness of the image than occurs with 4- to 5-month-olds, by showing no developmental changes in preference either for the scrambled images or the intact images when faces did not appear. Our results suggest that infants aged 6 months or more utilize scenic information for face processing.
No evidence for trans-generational immune priming in Drosophila melanogaster
Authors: Radhika R. , Lazzaro Brian P.
Published: July 2023

Most organisms are under constant and repeated exposure to pathogens, leading to perpetual natural selection for more effective ways to fight-off infections. This could include the evolution of memory-based immunity to increase protection from repeatedly-encountered pathogens both within and across generations. There is mixed evidence for intra- and trans-generational priming in non-vertebrates, which lack the antibody-mediated acquired immunity characteristic of vertebrates. In this work, we tested for trans-generational immune priming in adult offspring of the fruit fly, Drosophila melanogaster, after maternal challenge with 10 different bacterial pathogens. We focused on natural opportunistic pathogens of Drosophila spanning a range of virulence from 10% to 100% host mortality. We infected mothers via septic injury and tested for enhanced resistance to infection in their adult offspring, measured as the ability to suppress bacterial proliferation and survive infection. We categorized the mothers into four classes for each bacterium tested: those that survived infection, those that succumbed to infection, sterile-injury controls, and uninjured controls. We found no evidence for trans-generational priming by any class of mother in response to any of the bacteria.
Capturing COVID-19 spread and interplay with multi-hop contact tracing intervention
Authors: Kim Jungyeol , Bidokhti Shirin Saeedi , Sarkar Saswati
Published: July 2023

A preemptive multi-hop contact tracing scheme that tracks not only the direct contacts of those who tested positive for COVID-19, but also secondary or tertiary contacts has been proposed and deployed in practice with some success. We propose a mathematical methodology for evaluating this preemptive contact tracing strategy that combines the contact tracing dynamics and the virus transmission mechanism in a single framework using microscopic Markov Chain approach (MMCA). We perform Monte Carlo (MC) simulations to validate our model and show that the output of our model provides a reasonable match with the result of MC simulations. Utilizing the formulation under a human contact network generated from real-world data, we show that the cost-benefit tradeoff can be significantly enhanced through an implementation of the multi-hop contact tracing as compared to traditional contact tracing. We further shed light on the mechanisms behind the effectiveness of the multi-hop testing strategy using the framework. We show that our mathematical framework allows significantly faster computation of key attributes for multi-hop contact tracing as compared to MC simulations. This in turn enables the investigation of these attributes for large contact networks, and constitutes a significant strength of our approach as the contact networks that arise in practice are typically large.
Feasibility study of Internet video-based speech-language activity for outpatients with primary progressive aphasia
Authors: Higashi Shinji , Koshibe Yuko , Miyazaki Takehiro , Watanabe Ryohei , Nakanome Hanako , Inoue Takeshi , Asada Takashi , Arai Tetsuaki
Published: July 2023

Background

Primary progressive aphasia is a clinical dementia syndrome secondary to neurodegenerative disease characterized by language-related difficulties. Currently, there is no effective treatment for language impairment in primary progressive aphasia. In the present study, we investigated the feasibility of Internet video-based speech-language activities for this condition.

Methods

Twenty-three people with primary progressive aphasia (pwPPA) participated in the study and were provided with twelve speech-language activity videos on a dedicated website, with three sessions per week. The group that chose to continue with participation after three months of intervention received Internet activities for one year. Cognitive domains associated with persistence, treatment motivation, and video difficulty settings were statistically analyzed.

Results

After three months, 17 out of 23 participants opted to continue with the activities. The ability to follow oral commands which was measured pre intervention was higher in the group that continued compared with those participants who discontinued activity. The scores of two Standard Language Test of Aphasia subtests, sentence repetition and narrative writing–associated with the ability to comprehend and produce sentence structure–were highly correlated with motivation, interest and concentration in activity. Participants with different levels of primary progressive aphasia progression could participate in the same video-based activities when high-frequency words were used in the video.

Conclusions

Internet video-based speech-language activity at home has potential as a useful tool for future primary progressive aphasia treatment because it provides a cost-effective approach to intensive intervention and overcomes barriers associated with traditional therapy approaches.
Effects of personal and interpersonal factors on changes of food choices and physical activity among college students
Authors: Cao Juan , Wang Kun , Shi YuHui , Pan YuQing , Lyu MoHan , Ji Ying , Zhang Yan
Published: July 2023

Background

Health behaviors developed in the college years tend to persist in adulthood. However, distinct changing patterns of food choices and physical activity (PA) and their predictors are still less clear among college students. The current study sought to explore changes of food choices and PA, as well as the effects of personal and interpersonal factors.

Method

Two-wave longitudinal data was collected from a sample of 431 Chinese college students (Mean baseline age = 19.15 ± 0.61 years; 45.7% male). A validated self-reported food frequency questionnaire was used to assess the frequency of food choices. The Chinese revised version of physical activity rating scale was used to assess physical activity. Latent profile analysis, latent transition analysis, and multinomial logistic regression analysis were used to analyze the data.

Results

Two profiles of food choices, i.e.,Avoiding staples(5.1% at Time 1) andVaried diet(94.9% at Time 1), were identified at both timepoints. 90.9% remained the same profiles over time, 63.6% participants in theAvoiding staplesprofile shifted to theVaried dietprofile, and only 6.3% of those in theVaried dietprofile shifted to theAvoiding staplesprofile. Negative body shape-related belief was related to the translation from theVaried dietprofile to theAvoiding staplesprofile. Further, four profiles of PA, i.e.,Inactives(51.0% at Time 1),Low activies(26.0% at Time 1),Moderate activies(15.3% at Time 1), andActivies(7.7% at Time 1), were identified at both timepoints. 50.8% remained the same profiles over time, 38.6%Inactiviesshifted to the other profiles, and 48.5%Activiesshifted to the other profiles over time. Participants with higher self-efficacy showed an increase in PA over time, and those with lower self-efficacy and lower peer support showed a decrease in PA over time.

Conclusions

Overall, most of college students remained the same food choices profiles, and body shape-related belief contributed to changes in food choices profiles. About half of college students experienced changes in PA, and the predictors of such changes were peer support and self-efficacy. The findings extend the understanding of the personal and interpersonal predictors of health behaviors among college students from a dynamic perspective.
A novel co-culture model for investigation of the effects of LPS-induced macrophage-derived cytokines on brain endothelial cells
Authors: Yang Junling , Li Yinchuan , Bhalla Ambuj , Maienschein-Cline Mark , Fukuchi Ken-ichiro
Published: July 2023

In order to study effects of macrophage-derived inflammatory mediators associated with systemic inflammation on brain endothelial cells, we have established a co-culture system consisting of bEnd.3 cells and LPS-activated Raw 264.7 cells and performed its cytokine profiling. The cytokine profile of the co-culture model was compared to that of mice treated with intraperitoneal LPS injection. We found that, among cytokines profiled, eight cytokines/chemokines were similarly upregulated in both in vivo mouse and in vitro co-culture model. In contrast to the co-culture model, the cytokine profile of a common mono-culture system consisting of only LPS-activated bEnd.3 cells had little similarity to that of the in vivo mouse model. These results indicate that the co-culture of bEnd.3 cells with LPS-activated Raw 264.7 cells is a better model than the common mono-culture of LPS-activated bEnd.3 cells to investigate the molecular mechanism in endothelial cells, by which systemic inflammation induces neuroinflammation. Moreover, fibrinogen adherence both to bEnd.3 cells in the co-culture and to brain blood vessels in a LPS-treated animal model of Alzheimer’s disease increased. To the best of our knowledge, this is the first to utilize bEnd.3 cells co-cultured with LPS-activated Raw 264.7 cells as an in vitro model to investigate the consequence of macrophage-derived inflammatory mediators on brain endothelial cells.
Informal ready-to-eat food vending governance in urban Nigeria: Formal and informal lenses guiding the practice
Authors: Adeosun Kehinde Paul , Oosterveer Peter , Greene Mary
Published: July 2023

Informal ready-to-eat food vending is an important, cheap, convenient, accessible and readily available urban food supply sector that has become an increasingly important part of the diets of people in developing cities in Africa and throughout other contexts in the Global South. Over decades, despite challenges associated with health and hygiene, street foods have been informally accepted as part of the urban food supply system, particularly among the urban poor. Despite the importance of street foods to food security and employment needs in urban Nigeria and elsewhere, very little is known about the governance arrangements (whether formal or informal) revolving around their food provisioning practices. The paper explores governance arrangements that steer and shape food provisioning practices in Ibadan, Nigeria. Taking a social practice approach, the paper analyses the interconnections between governance and ready-to-eat food vending practices. It doing so, it draws on insights generated through a qualitative study incorporating in-depth interviews and participant observation methods to understand different governance arrangements revolving around food vending practices. The findings reveal that formal and informal governance structures are jointly steering and shaping practices of informal ready-to-eat food vending. They furthermore highlight the crucial role informal middlemen fulfill in informal food governance chains. These insights provide new avenues for thinking about food governance of urban food supply systems in terms of co-governance between formal and informal actors. They also provide empirical evidence that can aid policy application and implementation on urban food supply systems going forward. The paper concludes by discussing the potential of a co-governance informal food sector framework that recognizes and encompasses the formal-informal nature of the food sector. Such an approach recognizes and involves informal middlemen in the governance of informal ready-to-eat street food vending embedded in a larger framework of food system governance.
Genomic and phenotypic characterization of Pseudomonas sp. GOM7, a novel marine bacterial species with antimicrobial activity against multidrug-resistant Staphylococcus aureus
Authors: Romero-González Luis E. , Rojas-Vargas Jorge , Muriel-Millán Luis F. , Bustos-Martínez Jaime , Bustamante Víctor H. , Pardo-López Liliana
Published: July 2023

Antimicrobial resistance (AMR) represents a serious threat to global health. The development of new drugs to combat infections caused by bacteria resistant to multiple or even all available antibiotics is urgent. Most antibiotics used up to date have been identified from soil microorganisms. The marine environment represents an alternative source with great potential for the identification of microorganisms that produce bioactive molecules, including antibiotics. In this study, we analyzed the antibacterial activity of a collection of 82 bacterial strains isolated from marine water and sediment samples collected from the Southwestern Gulf of Mexico. Eight of the marine isolates inhibited the growth of different pathogenic bacteria, seven of which were identified as presumptive Pseudomonas aeruginosa. Interestingly, genome sequencing and phylogenetic analysis revealed that the remaining marine isolate showing antibacterial activity is a novel Pseudomonas species that we denominated Pseudomonas sp. GOM7, which was not pathogenic in the Galleria mellonella infection model in the conditions tested. Notably, Pseudomonas sp. GOM7 inhibited the growth of multidrug and methicillin-resistant strains of the priority pathogen Staphylococcus aureus. Our results show that the anti-S. aureus compound(s) produced by Pseudomonas sp. GOM7 can be extracted from the culture supernatant of this bacterium with the organic solvent ethyl acetate. Annotation of the Pseudomonas sp. GOM7 genome revealed the presence of several biosynthetic gene clusters predicted to code for possible antimicrobial compounds. Our results further highlight the potential of bacteria from the Gulf of Mexico as a source of novel antimicrobials.
Exposure levels of animal allergens, endotoxin, and β-(1,3)-glucan on a university campus of veterinary medicine
Authors: Zahradnik Eva , Sander Ingrid , Lotz Anne , Liebers Verena , Thullner Ingrid , Tacke Sabine , Raulf Monika
Published: July 2023

Objectives

The study aimed to determine the allergen, endotoxin and β-(1,3)-glucan concentrations at various areas on a university campus of veterinary medicine.

Methods

Dust samples were collected four times a year for three years using electrostatic dust collectors (EDC) at 25 different locations on a campus of veterinary medicine and in laboratories of inorganic chemistry as a control area representing animal-free environment. Major animal allergens from dog, cat, horse, cattle and mouse, domestic mite (DM) allergens, and β-(1,3)-glucan were measured using enzyme immunoassays and endotoxin using the limulus amoebocyte lysate (LAL) assay. Seasonal, annual and local influences on exposure levels were analyzed using Bayesian mixed models.

Results

With the exception of mouse allergens, all other determinants were found in almost all locations on the campus and in the control area, but in up to 10.000-fold variable concentrations. By far the highest levels of feline, canine, equine and bovine allergens were detected in buildings where the respective species were examined. The highest levels of mouse and DM allergens, β-(1,3)-glucan and endotoxin occurred together and were associated with locations where large animals were present. In buildings without animals, allergen levels were considerably lower but still elevated at several locations compared to the control area, especially for dog and horse allergens, and β-(1,3)-glucan. Significant seasonal effects were observed for dog, cat, horse and DM allergens, and β-(1,3)-glucan. Variations between years were less apparent than between seasons (except for β-(1,3)-glucan).

Conclusions

The strongest influencing factor on the concentration of mammalian allergens was the presence of the corresponding animal at the collection site. Seasonal influence on allergen concentrations was observed, while the overall exposure remained constant over the years. At locations with horses, elevated levels of mite allergens, endotoxin, and β-(1,3)-glucan can be expected, probably due to passive transfer from stable environment.