bluuebunny/crossref_metadata_2025 huggingface dataset:
Leases for Lives
Authors: Bellhouse David R.
Published: July 2017

Many historians of insurance have commented on the disconnect between the rise of English life insurance companies in the early eighteenth century and the mathematics behind the sound pricing of life insurance products that was developed at about the same time. Insurance and annuity promoters typically ignored this mathematical work. Bellhouse explores this issue, and shows that the early mathematical work was not motivated by insurance but instead by the fair valuation of life contingent contracts related to property. Even the work of the mathematician James Dodson in the creation of the Equitable Life Assurance Society, offering sound actuarially based premiums, did not change the industry in any significant way. The tipping point was a crisis in 1770 in which the philosopher and mathematician Richard Price, as well as other mathematicians, showed that a dozen or more recently formed annuity societies could not meet their financial obligations and were inviable.
Montazeri
Authors: Siavoshi Sussan
Published: July 2017

By the time of his death in 2009, the Grand Ayatollah Montazeri was lauded as the spiritual leader of the Green movement in Iran. Since the 1960s, when he supported Ayatollah Khomeini's opposition to the Shah, Montazeri's life reflected the crucial political shifts within Iran. In this book, Sussan Siavoshi presents the historical context as well as Montazeri's own political and intellectual journey. Siavoshi highlights how Montazeri, originally a student of Khomeini became one of the key figures during the revolution of 1978–9. She furthermore analyses his subsequent writings, explaining how he went from trusted advisor to and nominated successor of Khomeini to an outspoken critic of the Islamic Republic. Examining Montazeri's political thought and practice as well as the historical context, Siavoshi's book is vital for those interested in post-revolutionary Iran and the phenomenon of political Islam.
‘HIPPIAS, HANDSOME AND WISE’: A NOTE ON A BON MOT IN PLATO, HP. MAI. 281A1
Authors: Destrée Pierre
Published: July 2017

Plato's Hippias Major has usually been taken to be a comic dialogue, and rightly so. Its main theme is the καλόν, but what is primarily targeted and harshly mocked throughout the dialogue is Hippias’ pretence of having σοφία, which should allow him to define what the καλόν consists in. Yet, καλόν is an ambiguous term since, besides its aesthetic meaning, it also usually means the ‘morally right’. Not being able to define what καλόν is therefore also amounts to being unable to define what the right is. And indeed, the genuine σοφία, as Plato will tell us explicitly, is the σοφία that helps people, especially the youth, to become morally better (see especially 283c4, where Socrates has Hippias wholeheartedly admitting that his σοφία is supposed to aim at εἰς ἀρετὴν βελτίους ποιεῖν). Thus, the serious conclusion Plato wants his reader to draw is, first, that the well-known sophist Hippias (and perhaps all the sophists, more generally) have no real σοφία despite their very name, and second, and most importantly, that they cannot help anyone become virtuous, and that therefore their claim of educating people in moral goodness proves to be specious.
SALAFI THOUGHT IN TURKISH PUBLIC DISCOURSE SINCE 1980
Authors: Hammond Andrew
Published: July 2017

Turkey has been absent from the growing literature on the phenomenon of transnational Salafism. A tendency among Middle East specialists to focus on Arab regions and in Turkey on the Islamist movement and its long struggle with the Kemalist establishment has perpetuated the notion of Turkey as a category apart. This article argues that, on the contrary, Salafism is a fringe strand of Turkish Islam that began to evolve in the context of the state's effort in the 1980s to recalibrate religion as a complement to nationalism. Salafism became a topic of discussion in media and scholarly writing in Turkish religious studies faculties, while self-styled Salafi preachers trained in Saudi Arabia found a niche through publishing houses. These publishers facilitated the translation into Turkish of Arabic texts by important Saudi religious scholars in an effort to change the discursive landscape of Islam in Turkey. I show that contra assumptions of a rich Sufi tradition acting as a block against modern Salafi ideas, Salafism managed to gain a foothold in Turkey, facilitated in part by the republic's experience of secular materialism.
New Asymmetries in the New Authoritarianism: Research in Egypt in the Age of Post-Revolution
Authors: Ryzova Lucie
Published: July 2017

Egypt was at the center of a wave of uprisings and revolutions that swept the region between 2011 and 2013, the common denominator of which was demands for a radical democratic alternative to authoritarian regimes variously formulated around social justice and political rights. While the Middle East was a major theater of these events, with Tunisia, Egypt, Lybia, Syria, Bahrain, and Yemen sharing the headlines, the processes that informed these uprisings were also deeply global. The year 2011 was a revolutionary year, maybe the last in history, when actors differently positioned in the neoliberal social landscape mobilized in different ways, from the Occupy Movement to the London riots. The demise, or better, defeat, of these movements has reverberated profoundly around the globe, highlighting the postdemocratic nature of governance in contemporary states. One of the effects of the rise of new authoritarianism across Europe and the United States is a palpable transformation in the asymmetry between outside observer and the local observed. Researchers now face a reshaping, in some ways a leveling, of differences between “us” and “them” and the distinct temporality used to underpin this asymmetry. Nothing could illustrate this better than the fact that as I write, Egypt's president ʿAbd al-Fattah al-Sisi is enjoying a warm welcome in the White House. The narrative is no longer framed through the worn-out trope of an Arab leader aspiring to modernize his country through pledging allegiance to the leader of the Free World in exchange for aid and armaments; now the man in the White House implicitly pledges to learn from the Arab dictator. Egypt is the pioneer; the United States is the relative latecomer to the Age of New Authoritarianism.
Out of Space: Securitization, Intimacy, and New Research Challenges in Post-2009 Iran
Authors: Saeidi Shirin , Rivetti Paola
Published: July 2017

In post-2009 Iran, not only is space gendered for a variety of reasons ranging from customs to state intervention, but also public space has become less accessible and secluded for security purposes. To securitize the state or replace a sense of trust with that of suspicion, states blend the gendering of space with the architecture of seclusion. In the United States, for instance, the separation of males and females in the prison industrial complex includes seclusion of bodies and often subjects gender-nonconforming people, immigrants, and those with HIV to disproportionate levels of physical danger. In Iran, architectural adjustments with the aim of seclusion have significantly increased since the 2009 protests. In Tehran, for instance, shisha shops in the mountains, which used to be common sites of leisure, are randomly raided by security forces. As a result, participating in such spaces means having to hide in the back areas to engage in an activity that not too long ago was legal. It follows that the combination of gendering and seclusion of space disrupts the formation of organic relationships and generates real, falsely stimulated, and contested intimacies. How we approach intimacies in this complicated situation determines in important ways the impact that this new spatial scheme will have on our research agenda, analysis, and perhaps even safety.
The Archive as a “Collective Project”
Authors: Özok-Gündoğan Nilay
Published: July 2017

The history of the archive is the history of the state. Or so say conventional approaches to the archives. Until recently, the archive has been seen solely as a site, or rather a repository, of modern state power and governmentality, and a crucial medium for the making and preservation of national memory in the late 19th century. There is a truth to this state-centric perspective: the archive was conceived as a place where governments keep their records; they usually contain a term such as “state,” “government,” or “national” in their names; and they are often funded by and connected to a governmental body.
Intermediate input linkage and carbon leakage
Authors: Zhang Zengkai , Zhang Zhongxiang
Published: July 2017

Climate regulations tend to target energy-intensive sectors whose products are widely used in industrial production as intermediate inputs, and carbon abatement may be partially offset by intermediate input-led leakage. This paper aims to examine the impact of intermediate input linkages on carbon leakage both theoretically and empirically. The theoretical part develops a Harberger-type model with an input-output linkage structure, identifies four leakage effects and derives closed-form solutions for these leakage effects. Its empirical part builds a computable general equilibrium model of China's economy and introduces structural decomposition analysis to link the theoretical and empirical models. When imposing a carbon price on the electricity generation sector, our results show significant sectoral carbon leakage. Our decomposition analysis further suggests that such leakage is mainly through the production substitution effect and the multiplier effect. Our results highlight the importance of sectoral linkage when discussing the carbon leakage issue of climate policies.
Genome-wide SNPs detection by genomic comparison between Dongxiang wild rice (Oryza rufipogon) and cultivated rice Nipponbare (Oryza sativa ssp. japonica)
Authors: Zhang Meng , Huang Wenyang , Xia Zijun , Liu Jiahui , Xie Jiankun , Zhang Fantao
Published: July 2017

Dongxiang wild rice (Oryza rufipogon, DXWR) exhibits valuable agronomic traits and represents a precious germplasm resource for rice breeding. The use of genetic markers can greatly speed up the breeding process and facilitate research on genetics and genomics. In our previous study, we identified insertion–deletion polymorphisms between DXWR and cultivated rice Nipponbare (Oryza sativa ssp. japonica), using whole-genome sequencing in DXWR. In this study, to further explore the genetic variations and enrich the available genetic markers of DXWR, we identified 1,089,478 single-nucleotide polymorphisms (SNPs) (corresponding to one SNP per 0.33 kb of the genome) by genomic comparison between DXWR and Nipponbare, using the genome sequencing data and bioinformatics approaches. Furthermore, the accuracy of the identified SNPs was also validated by polymerase chain reaction amplification and Sanger sequencing. This genome-wide SNPs identification greatly increases the number of genetic markers available for DXWR and provides new opportunities to exploit this valuable and endangered germplasm resource.
Crop Biomass Not Species Richness Drives Weed Suppression in Warm-Season Annual Grass–Legume Intercrops in the Northeast
Authors: Bybee-Finley K. Ann , Mirsky Steven B. , Ryan Matthew R.
Published: July 2017

Intercropping with functionally diverse crops can reduce the availability of resources that could otherwise be used by weeds. An experiment was conducted across 6 site-years in New York and Maryland in 2013 and 2014 to examine the effects of functional diversity and crop species richness on weed suppression. We compared four annual crop species that differed in stature and nitrogen acquisition traits: (1) pearl millet, (2) sorghum sudangrass, (3) cowpea, and (4) sunn hemp. Crops were seeded in monoculture and in three- and four-species mixtures using a replacement design in which monoculture seeding rates were divided by the number of species in the intercrop. Crop and weed biomass were sampled at ~45 and 90 d after planting. At the first sampling date, intercrops produced more crop biomass than monocultures in all but 1 site-year; however, weed biomass in intercrops was lower than monocultures in only 1 site-year. By the second sampling date, crop biomass was consistently greater in the intercrops than in the monocultures, and weed biomass was lower in the intercrops than in monocultures in 2 site-years. Although we observed several negative relationships between crop species richness and weed biomass, crop biomass was a more important factor than species richness for suppressing weeds. Despite the weak weed suppression from the two legumes compared with the two grasses, legume crops can provide other benefits, including increased forage quality, soil nitrogen for subsequent crops, and resources for pollinators if allowed to flower. On the other hand, if weed suppression is the top priority, our results suggest that monocultures of high biomass–producing grasses will provide more effective suppression at a lower seed cost than functionally diverse intercrops that include low biomass–producing legumes in warm-season intercrops.
CME: Polycythemia vera, myeloproliferative Neoplasie
Authors: Bächli Esther , Gundelfinger Micha
Published: July 2017

Zusammenfassung. Zusammenfassung: Myeloproliferative Neoplasien (MPN) nach den Kriterien der WHO 2016 [1] stellen eine heterogene Krankheitsgruppe dar, die durch Proliferation einer oder mehrerer hämatopoietischer Zellreihen definiert ist. Diese Krankheiten können sich mit unspezifischen Symptomen manifestieren, und oft führen Zufallsbefunde bei Blutanalysen zur Diagnose. Zu den MPN gehören die Polycythemia vera, die essenzielle Thrombozytose, die präfibrotische Osteomyelofibrose, die Osteomyelofibrose und die chronische myeloische Leukämie. Im Folgenden wird auf die Polycythemia vera (PV) und ihre Differenzialdiagnosen eingegangen.
Aktuelle Behandlungskonzepte bei Belastungsinkontinenz
Authors: Rautenberg Oliver , Zivanovic Irena , Kociszewski Jacek , Kuszka Andrzej , Münst Julia , Eisele Lilly , Viereck Nicole , Walser Claudia , Gamper Marianne , Viereck Volker
Published: July 2017

Zusammenfassung. Zusammenfassung: Eine Belastungsinkontinenz sollte immer zuerst konservativ behandelt werden. Schon eine Gewichtsreduktion, Hormonpräparate, Physiotherapie, Beckenbodentraining und/oder die Anwendung von Pessaren können zum Erfolg führen. Nach Ausschöpfen dieser Therapien werden heute Inkontinenzoperationen mit meist sehr guten Heilungschancen (ca. 80–90 %) angeboten. Der operative Goldstandard ist die suburethrale Schlingeneinlage. Die Pelvic-Floor-Sonografie liefert dazu sehr wichtige Hinweise zur Wahl der Operationstechnik und zur Behebung von Komplikationen. Ferner bildet die intra- oder paraurethrale Injektion von Bulking Agents eine vielversprechende, wenig invasive operative Alternative. In diesem Artikel werden Behandlungskonzepte, prä-, intra- und postoperative Untersuchungen, Wahl der Operationsmethode, operationstechnische Details für den Operationserfolg sowie Vorbeugung und Behandlung von Komplikationen diskutiert.
Are Humans to Blame for Worsening Heat Waves in China?
Authors: Underwood Emily
Published: July 2017

A new study suggests that even hotter events will follow unless greenhouse gas emissions are reduced.
Hubble Reveals Less Studied Regions of Jupiter’s Auroras
Authors: Witman Sarah
Published: July 2017

With a dose of fiery plasma, the secondary arcs of Jupiter’s aurora shine bright.
Metabonomics Approach to Assessing the Modulatory Effects of Kisspeptin-10 on Liver Injury Induced by Heat Stress in Rats
Authors: Hou Yuanlong , Wang Xiaoyan , Ping Jihui , Lei Zhihai , Gao Yingdong , Ma Zhiyu , Jia Cuicui , Zhang Zheng , Li Xiang , Jin Mengmeng , Li Xiaoliang , Suo Chuan , Zhang Ying , Su Juan
Published: July 2017

The protective effects of Kisspeptin on heat-induced oxidative stress in rats were investigated by using a combination of biochemical parameters and metabonomics. Metabonomic analyses were performed using gas chromatography/mass spectrometry in conjunction with multivariate and univariate statistical analyses. At the end point of the heat stress experiment, histological observation, ultrastructural analysis and biochemical parameters were measured. Metabonomic analysis of liver tissue revealed that Kisspeptin mainly attenuated the alteration of purine metabolism and fatty acid metabolism pathways. Futhermore, Kisspeptin also increased the levels of GSH, T-AOC as well as SOD activities, and upregulated MDA levels. These results provide important mechanistic insights into the protective effects of Kisspeptin against heat-induced oxidative stress.
Contrast-Enhanced Ultrasound Imaging Quantification of Adventitial Vasa Vasorum in a Rabbit Model of Varying Degrees of Atherosclerosis
Authors: Li Xiaoying , Zhang Ruyou , Li Zongmin , Ning Chunping , Wang Zhenzhen , Dang Meizheng , Peng Yanqing , Han Xuesong , Sun Litao , Tian Jiawei
Published: July 2017

This study used an atherosclerotic rabbit model to investigate the feasibility of quantifying adventitial vasa vasorum (VV) via contrast-enhanced ultrasound (CEUS) imaging to identify early atherosclerosis. Recent evidence has linked adventitial VV with atherosclerotic plaque progression and vulnerability. A growth in VV density has been detected preceding intimal thickening and even endothelial dysfunction. In our study, carotid atherosclerosis rabbit models were used, and animals underwent CEUS imaging at the end of the atherosclerotic induction period. Normalized maximal video-intensity enhancement (MVE) was calculated to quantify VV density. After CEUS imaging, animals were euthanized, and their carotids were processed for histopathological analysis following staining for CD31 and VEGF. Adventitial normalized MVE increased as atherosclerosis progressed (p < 0.001), and normalized MVE also progressed, demonstrating a linear correlation with histological findings (r = 0.634, p < 0.001 for VEGF-positive; r = 0.538, p < 0.001 for CD31-positive). Thus, we histologically validated that CEUS imaging can be used to quantify the development of adventitial VV associated with atherosclerosis progression. This method can be used for monitoring the VV to detect early atherosclerosis.
Greenhouse gas emissions as a result of spectators travelling to football in England
Authors: Dosumu Adekunle , Colbeck Ian , Bragg Rachel
Published: July 2017

Transport remains a critical avenue in the attempt to reduce greenhouse gas (GHG) emissions and any significant effort to reduce travel GHG emissions will need to encourage a movement towards more fuel-efficient, less polluting behaviours. The aim of this paper is to calculate GHG emissions arising from the travel of spectators to and from football games within eight football tiers (3 to 10) in England, and to extrapolate this to a national level. The study comprised of 1649 participants with an average age of 42 years (M = 42.63, SD = 17.10). Participants travelled to and from games by walking, cycling, car, bus, train or taxi. The average distance travelled to and from games was 41.5 km. A Kruskal-Wallis test was conducted to evaluate differences in travel related GHG emissions between the eight football tiers during the 2012/13 season. The results indicate significant differences between football tiers’ GHG emissions, H(7) = 46.474, p < 0.001. The annual GHG emission of spectators from the 8 tiers for the 2012/13 season was estimated at 56,237 tonnes of CO2e, accounting for less than 0.05% of transport emissions in England. Football authorities should have robust travel plans and educate spectators to employ more sustainable travel plans to games.
Evaluation of realistic layouts for next generation on-scalp MEG: spatial information density maps
Authors: Riaz Bushra , Pfeiffer Christoph , Schneiderman Justin F.
Published: July 2017

While commercial magnetoencephalography (MEG) systems are the functional neuroimaging state-of-the-art in terms of spatio-temporal resolution, MEG sensors have not changed significantly since the 1990s. Interest in newer sensors that operate at less extreme temperatures, e.g., high critical temperature (high-Tc) SQUIDs, optically-pumped magnetometers, etc., is growing because they enable significant reductions in head-to-sensor standoff (on-scalp MEG). Various metrics quantify the advantages of on-scalp MEG, but a single straightforward one is lacking. Previous works have furthermore been limited to arbitrary and/or unrealistic sensor layouts. We introduce spatial information density (SID) maps for quantitative and qualitative evaluations of sensor arrays. SID-maps present the spatial distribution of information a sensor array extracts from a source space while accounting for relevant source and sensor parameters. We use it in a systematic comparison of three practical on-scalp MEG sensor array layouts (based on high-Tc SQUIDs) and the standard Elekta Neuromag TRIUX magnetometer array. Results strengthen the case for on-scalp and specifically high-Tc SQUID-based MEG while providing a path for the practical design of future MEG systems. SID-maps are furthermore general to arbitrary magnetic sensor technologies and source spaces and can thus be used for design and evaluation of sensor arrays for magnetocardiography, magnetic particle imaging, etc.
Neuronal patterning of the tubular collar cord is highly conserved among enteropneusts but dissimilar to the chordate neural tube
Authors: Kaul-Strehlow Sabrina , Urata Makoto , Praher Daniela , Wanninger Andreas
Published: July 2017

A tubular nervous system is present in the deuterostome groups Chordata (cephalochordates, tunicates, vertebrates) and in the non-chordate Enteropneusta. However, the worm-shaped enteropneusts possess a less complex nervous system featuring only a short hollow neural tube, whereby homology to its chordate counterpart remains elusive. Since the majority of data on enteropneusts stem from the harrimaniid Saccoglossus kowalevskii, putative interspecific variations remain undetected resulting in an unreliable ground pattern that impedes homology assessments. In order to complement the missing data from another enteropneust family, we investigated expression of key neuronal patterning genes in the ptychoderid Balanoglossus misakiensis. The collar cord of B. misakiensis shows anterior Six3/6 and posterior Otx + Engrailed expression, in a region corresponding to the chordate brain. Neuronal Nk2.1/Nk2.2 expression is absent. Interestingly, we found median Dlx and lateral Pax6 expression domains, i.e., a condition that is reversed compared to chordates. Comparative analyses reveal that adult nervous system patterning is highly conserved among the enteropneust families Harrimaniidae, Spengelidae and Ptychoderidae. BmiDlx and BmiPax6 have no corresponding expression domains in the chordate brain, which may be indicative of independent acquisition of a tubular nervous system in Enteropneusta and Chordata.
Simultaneous determination of hydrophilic and lipophilic constituents in herbal medicines using directly-coupled reversed-phase and hydrophilic interaction liquid chromatography-tandem mass spectrometry
Authors: Sun Wan-Yang , Lu Qin-Wei , Gao Hao , Tong Ling , Li Dong-Xiang , Zhou Zheng-Qun , Jiang Zheng-Jin , Sun Henry , Bi Kai-Shun
Published: July 2017

Limitations in the separation ability of conventional liquid chromatography system remains a challenge in developing a versatile method for simultaneously determining both hydrophilic and lipophilic constituents in herbal medicines (HMs). To measure compounds covering a broad polarity span in HMs, we developed a directly-coupled reversed-phase and hydrophilic interaction liquid chromatography-tandem mass spectrometry system. Samples were firstly separated according to lipophilicity by using a C18 column. Utilizing a T-piece as connector, the eluent was then pumped into an amide column to get further separation that mainly based on the hydrogen bonding effects. Dan-Qi pair, an extensively used herb-combined prescription in China, was selected to test the practicability and performance of the established system. A total of 27 components, containing 9 hydrophilic and 18 lipophilic constituents, were simultaneously determined using a schedule multiple reaction monitoring method in 15 min. Up to 69.9% content could be monitored in one injection in Dan-Qi pair extract, showing a significant advantage over previous methods. The proposed method was expected to benefit the controllability of herbal medicines.
Experimental evidence of high pressure decoupling between charge transport and structural dynamics in a protic ionic glass-former
Authors: Wojnarowska Z. , Rams-Baron M. , Knapik-Kowalczuk J. , Połatyńska A. , Pochylski M. , Gapinski J. , Patkowski A. , Wlodarczyk P. , Paluch M.
Published: July 2017

In this paper the relaxation dynamics of ionic glass-former acebutolol hydrochloride (ACB-HCl) is studied as a function of temperature and pressure by using dynamic light scattering and broadband dielectric spectroscopy. These unique experimental data provide the first direct evidence that the decoupling between the charge transport and structural relaxation exists in proton conductors over a wide T-P thermodynamic space, with the time scale of structural relaxation being constant at the liquid-glass transition (τα = 1000 s). We demonstrate that the enhanced proton transport, being a combination of intermolecular H+ hopping between cation and anion as well as tautomerization process within amide moiety of ACB molecule, results in a breakdown of the Stokes-Einstein relation at ambient and elevated pressure with the fractional exponent k being pressure dependent. The dT
g
/dP coefficient, stretching exponent βKWW and dynamic modulus E
a
/ΔV
#
were found to be the same regardless of the relaxation processes studied. This is in contrast to the apparent activation volume parameter that is different when charge transport and structural dynamics are considered. These experimental results together with theoretical considerations create new ideas to design efficient proton conductors for potential electrochemical applications.
Integrative whole-genome sequence analysis reveals roles of regulatory mutations in BCL6 and BCL2 in follicular lymphoma
Authors: Batmanov Kirill , Wang Wei , Bjørås Magnar , Delabie Jan , Wang Junbai
Published: July 2017

The contribution of mutations in regulatory regions to tumorigenesis has been the subject of many recent studies. We propose a new framework for integrative analysis of genome-wide sequencing data by considering diverse genetic information. This approach is applied to study follicular lymphoma (FL), a disease for which little is known about the contribution of regulatory gene mutations. Results from a test FL cohort revealed three novel highly recurrent regulatory mutation blocks near important genes implicated in FL, BCL6 and BCL2. Similar findings were detected in a validation FL cohort. We also found transcription factors (TF) whose binding may be disturbed by these mutations in FL: disruption of FOX TF family near the BCL6 promoter may result in reduced BCL6 expression, which then increases BCL2 expression over that caused by BCL2 gene translocation. Knockdown experiments of two TF hits (FOXD2 or FOXD3) were performed in human B lymphocytes verifying that they modulate BCL6/BCL2 according to the computationally predicted effects of the SNVs on TF binding. Overall, our proposed integrative analysis facilitates non-coding driver identification and the new findings may enhance the understanding of FL.
Blood-testis barrier integrity depends on Pin1 expression in Sertoli cells
Authors: Islam Rabia , Yoon Heein , Kim Bong-soo , Bae Han-sol , Shin Hye-rim , Kim Woo-Jin , Yoon Won-joon , Lee Yun-Sil , Woo Kyung Mi , Baek Jeong-Hwa , Ryoo Hyun-Mo
Published: July 2017

The conformation and function of a subset of serine and threonine-phosphorylated proteins are regulated by the prolyl isomerase Pin1 through isomerization of phosphorylated Ser/Thr-Pro bonds. Pin1 is intensely expressed in Sertoli cells, but its function in this post mitotic cell remains unclear. Our aim was to investigate the role of Pin1 in the Sertoli cells. Lack of Pin1 caused disruption of the blood-testis barrier. We next investigated if the activin pathways in the Sertoli cells were affected by lack of Pin1 through immunostaining for Smad3 protein in testis tissue. Indeed, lack of Pin1 caused reduced Smad3 expression in the testis tissue, as well as a reduction in the level of N-Cadherin, a known target of Smad3. Pin1−/− testes express Sertoli cell marker mRNAs in a pattern similar to that seen in Smad3+/− mice, except for an increase in Wt1 expression. The resulting dysregulation of N-Cadherin, connexin 43, and Wt1 targets caused by lack of Pin1 might affect the mesenchymal–epithelial balance in the Sertoli cells and perturb the blood-testis barrier. The effect of Pin1 dosage in Sertoli cells might be useful in the study of toxicant-mediated infertility, gonadal cancer, and for designing male contraceptives.
Enhanced robustness of evolving open systems by the bidirectionality of interactions between elements
Authors: Ogushi Fumiko , Kertész János , Kaski Kimmo , Shimada Takashi
Published: July 2017

Living organisms, ecosystems, and social systems are examples of complex systems in which robustness against inclusion of new elements is an essential feature. A recently proposed simple model has revealed a general mechanism by which such systems can become robust against inclusion of elements with totally random interactions when the elements have a moderate number of links. The interaction is, however, in many systems often intrinsically bidirectional like for mutual symbiosis and competition in ecology. This study reports the strong reinforcement effect of the bidirectionality of the interactions on the robustness of evolving systems. We show that the system with purely bidirectional interactions can grow with twofold average degree, in comparison with the purely unidirectional system. This drastic shift of the transition point comes from the reinforcement of each node, not from a change in structure of the emergent system. For systems with partially bidirectional interactions we find that the regime of the growing phase gets expanded. In the dense interaction regime, there exists an optimum proportion of bidirectional interactions for the growth rate at around 1/3. In the sparsely connected systems, small but finite fraction of bidirectional links can change the system’s behaviour from non-growing to growing.
Development of an integrated Monte Carlo model for glioblastoma multiforme treated with boron neutron capture therapy
Authors: Moghaddasi Leyla , Bezak Eva
Published: July 2017

Glioblastomas (GBM) are notorious for their high fatality rate. Boron Neutron Capture Therapy (BNCT) being a biochemically targeted type of radiotherapy is a potent modality for GBM. In the current work, a BNCT treatment modelling framework for GBM was developed. Optimal Clinical Target Volume (CTV) margins for GBM-BNCT and the BNCT efficacy have been investigated. The model integrated a cell-based dosimetry model, an in-house-developed epithermal neutron beam model and previously-developed Microscopic Extension Probability (MEP) model. The system was defined as a cubic ICRP-brain phantom divided into 20 μm side voxels. The corresponding 10B concentrations in GBM and normal brain cells were applied. The in-silico model was irradiated with the epithermal neutron beam using 2 and 2.5 cm CTV margins. Results from the cell-based dosimetry and the MEP models were combined to calculate GBM cell survival fractions (SF) post BNCT and compared to x-ray radiotherapy (XRT) SFs. Compared to XRT, the SF within the beam decreased by five orders of magnitudes and the total SF was reduced three times following BNCT. CTV extension by 0.5 cm reduced the SF by additional (53.8 ± 0.3)%. In conclusion, BNCT results in a more efficient cell kill. The extension of the CTV margin, however, may not increase the treatment outcome significantly.
Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection
Authors: Lai Yen-Chung , Chuang Yung-Chun , Liu Ching-Chuan , Ho Tzong-Shiann , Lin Yee-Shin , Anderson Robert , Yeh Trai-Ming
Published: July 2017

Dengue is the most common mosquito-transmitted viral infection for which an improved vaccine is still needed. Although nonstructural protein-1 (NS1) immunization can protect mice against dengue infection, molecular mimicry between NS1 and host proteins makes NS1-based vaccines challenging to develop. Based on the epitope recognized by the anti-NS1 monoclonal Ab (mAb) 33D2 which recognizes a conserved NS1 wing domain (NS1-WD) region but not host proteins, we synthesized a modified NS1-WD peptide to immunize mice. We found that both mAb 33D2 and modified NS1-WD peptide immune sera could induce complement-dependent lysis of dengue-infected but not un-infected cellsin vitro. Furthermore, either active immunization with the modified NS1-WD peptide or passive transfer of mAb 33D2 efficiently protected mice against all serotypes of dengue virus infection. More importantly, dengue patients with more antibodies recognized the modified NS1-WD peptide had less severe disease. Thus, the modified NS1-WD peptide is a promising dengue vaccine candidate.
Anti-diabetic Effects of Clostridium butyricum CGMCC0313.1 through Promoting the Growth of Gut Butyrate-producing Bacteria in Type 2 Diabetic Mice
Authors: Jia Lingling , Li Dongyao , Feng Ninghan , Shamoon Muhammad , Sun Zhenghua , Ding Lei , Zhang Hao , Chen Wei , Sun Jia , Chen Yong Q
Published: July 2017

Patients with type 2 diabetes (T2D) have decreased butyrate-producing bacteria. We hypothesized that supplementation with butyrate-producing bacteria may exert beneficial effects on T2D. The current study investigated the effects of well-characterized butyrate-producing bacteria Clostridium butyricum CGMCC0313.1 (CB0313.1) on hyperglycemia and associated metabolic dysfunction in two diabetic mouse models. CB0313.1 was administered daily by oral gavage to leptin
db/db
mice for 5 weeks starting from 3 weeks of age, and to HF diabetic mice induced by high fat diet (HFD) plus streptozotocin (STZ) in C57BL/6J mice for 13 weeks starting from 4 weeks of age. CB0313.1 improved diabetic markers (fasting glucose, glucose tolerance, insulin tolerance, GLP-1 and insulin secretion), and decreased blood lipids and inflammatory tone. Furthermore, CB0313.1 reversed hypohepatias and reduced glucose output. We also found that CB0313.1 modulated gut microbiota composition, characterized by a decreased ratio of Firmicutes to Bacteroidetes, reduced Allobaculum bacteria that were abundant in HF diabetic mice and increased butyrate-producing bacteria. Changes in gut microbiota following CB0313.1 treatment were associated with enhanced peroxisome proliferator–activated receptor-γ (PPARγ), insulin signaling molecules and mitochondrial function markers. Together, our study suggests that CB0313.1 may act as a beneficial probiotic for the prevention and treatment of hyperglycemia and associated metabolic dysfunction.
Nanosecond laser coloration on stainless steel surface
Authors: Lu Yan , Shi Xinying , Huang Zhongjia , Li Taohai , Zhang Meng , Czajkowski Jakub , Fabritius Tapio , Huttula Marko , Cao Wei
Published: July 2017

In this work, we present laser coloration on 304 stainless steel using nanosecond laser. Surface modifications are tuned by adjusting laser parameters of scanning speed, repetition rate, and pulse width. A comprehensive study of the physical mechanism leading to the appearance is presented. Microscopic patterns are measured and employed as input to simulate light-matter interferences, while chemical states and crystal structures of composites to figure out intrinsic colors. Quantitative analysis clarifies the final colors and RGB values are the combinations of structural colors and intrinsic colors from the oxidized pigments, with the latter dominating. Therefore, the engineering and scientific insights of nanosecond laser coloration highlight large-scale utilization of the present route for colorful and resistant steels.
Conditional knockdown of hyaluronidase 2 in articular cartilage stimulates osteoarthritic progression in a mice model
Authors: Higuchi Yoshitoshi , Nishida Yoshihiro , Kozawa Eiji , Zhuo Lisheng , Arai Eisuke , Hamada Shunsuke , Morita Daigo , Ikuta Kunihiro , Kimata Koji , Ushida Takahiro , Ishiguro Naoki
Published: July 2017

The catabolism of hyaluronan in articular cartilage remains unclear. The aims of this study were to investigate the effects of hyaluronidase 2 (Hyal2) knockdown in articular cartilage on the development of osteoarthritis (OA) using genetic manipulated mice. Destabilization of the medial meniscus (DMM) model of Col2a promoter specific conditional Hyal2 knockout (Hyal
−/−
) mice was established and examined. Age related and DMM induced alterations of articular cartilage of knee joint were evaluated with modified Mankin score and immunohistochemical staining of MMP-13, ADAMTS-5, KIAA11199, and biotinylated- hyaluronan binding protein staining in addition to histomorphometrical analyses. Effects of Hyal2 suppression were also analyzed using explant culture of an IL-1α induced articular cartilage degradation model. The amount and size of hyaluronan in articular cartilage were higher in Hyal2
−/−
mice. Hyal2
−/−
mice exhibited aggravated cartilage degradation in age-related and DMM induced mice. MMP-13 and ADAMTS-5 positive chondrocytes were significantly higher in Hyal2
−/−
mice. Articular cartilage was more degraded in explant cultures obtained from Hyal2
−/−
mice. Knockdown of Hyal2 in articular cartilage induced OA development and progression possibly mediated by an imbalance of HA metabolism. This suggests that Hyal2 knockdown exhibits mucopolysaccharidosis-like OA change in articular cartilage similar to Hyal1 knockdown.
Value of shear wave arrival time contour display in shear wave elastography for breast masses diagnosis
Authors: Zhou Bang-Guo , Wang Dan , Ren Wei-Wei , Li Xiao-Long , He Ya-Ping , Liu Bo-Ji , Wang Qiao , Chen Shi-Gao , Alizad Azra , Xu Hui-Xiong
Published: July 2017

To evaluate the diagnostic performance of shear wave arrival time contour (SWATC) display for the diagnosis of breast lesions and to identify factors associated with the quality of shear wave propagation (QSWP) in breast lesions. This study included 277 pathologically confirmed breast lesions. Conventional B-mode ultrasound characteristics and shear wave elastography parameters were computed. Using the SWATC display, the QSWP of each lesion was assigned to a two-point scale: score 1 (low quality) and score 2 (high quality). Binary logistic regression analysis was performed to identify factors associated with QSWP. The area under the receiver operating characteristic curve (AUROC) for QSWP to differentiate benign from malignant lesions was 0.913, with a sensitivity of 91.9%, a specificity of 90.7%, a positive predictive value (PPV) of 74.0%, and a negative predictive value (NPV) of 97.5%. Compared with using the standard deviation of shear wave speed (SWSSD) alone, SWSSD combined with QSWP increased the sensitivity from 75.8% to 93.5%, but decreased the specificity from 95.8% to 89.3% (P < 0.05). SWSSD was identified to be the strongest factor associated with the QSWP, followed by tumor malignancy and the depth of the lesion. In conclusion, SWATC display may be useful for characterization of breast lesions.
Martian slope streaks as plausible indicators of transient water activity
Authors: Bhardwaj Anshuman , Sam Lydia , Martín-Torres F. Javier , Zorzano María-Paz , Fonseca Ricardo M.
Published: July 2017

Slope streaks have been frequently observed in the equatorial, low thermal inertia and dusty regions of Mars. The reason behind their formation remains unclear with proposed hypotheses for both dry and wet mechanisms. Here, we report an up-to-date distribution and morphometric investigation of Martian slope streaks. We find: (i) a remarkable coexistence of the slope streak distribution with the regions on Mars with high abundances of water-equivalent hydrogen, chlorine, and iron; (ii) favourable thermodynamic conditions for transient deliquescence and brine development in the slope streak regions; (iii) a significant concurrence of slope streak distribution with the regions of enhanced atmospheric water vapour concentration, thus suggestive of a present-day regolith-atmosphere water cycle; and (iv) terrain preferences and flow patterns supporting a wet mechanism for slope streaks. These results suggest a strong local regolith-atmosphere water coupling in the slope streak regions that leads to the formation of these fluidised features. Our conclusions can have profound astrobiological, habitability, environmental, and planetary protection implications.
Patterns and drivers of daily bed-level dynamics on two tidal flats with contrasting wave exposure
Authors: Hu Zhan , Yao Peng , van der Wal Daphne , Bouma Tjeerd J.
Published: July 2017

Short-term bed-level dynamics has been identified as one of the main factors affecting biota establishment or retreat on tidal flats. However, due to a lack of proper instruments and intensive labour involved, the pattern and drivers of daily bed-level dynamics are largely unexplored in a spatiotemporal context. In this study, 12 newly-developed automatic bed-level sensors were deployed for nearly 15 months on two tidal flats with contrasting wave exposure, proving an unique dataset of daily bed-level changes and hydrodynamic forcing. By analysing the data, we show that (1) a general steepening trend exists on both tidal flats, even with contrasting wave exposure and different bed sediment grain size; (2) daily morphodynamics level increases towards the sea; (3) tidal forcing sets the general morphological evolution pattern at both sites; (4) wave forcing induces short-term bed-level fluctuations at the wave-exposed site, but similar effect is not seen at the sheltered site with smaller waves; (5) storms provoke aggravated erosion, but the impact is conditioned by tidal levels. This study provides insights in the pattern and drivers of daily intertidal bed-level dynamics, thereby setting a template for future high-resolution field monitoring programmes and inviting in-depth morphodynamic modelling for improved understanding and predictive capability.
PRR2, a pseudo-response regulator, promotes salicylic acid and camalexin accumulation during plant immunity
Authors: Cheval C. , Perez M. , Leba L. J. , Ranty B. , Perochon A. , Reichelt M. , Mithöfer A. , Robe E. , Mazars C. , Galaud J. P. , Aldon D.
Published: July 2017

Calcium signalling mediated by Calmodulin (CaM) and calmodulin-like (CML) proteins is critical to plant immunity. CaM and CML regulate a wide range of target proteins and cellular responses. While many CaM-binding proteins have been identified, few have been characterized for their specific role in plant immunity. Here, we report new data on the biological function of a CML-interacting partner, PRR2 (PSEUDO-RESPONSE REGULATOR 2), a plant specific transcription factor. Until now, the physiological relevance of PRR2 remained largely unknown. Using a reverse genetic strategy in A. thaliana, we identified PRR2 as a positive regulator of plant immunity. We propose that PRR2 contributes to salicylic acid (SA)-dependent responses when challenged with the phytopathogenic bacterium Pseudomonas syringae. PRR2 is transcriptionally upregulated by SA and P. syringae, enhances SA biosynthesis and SA signalling responses; e.g. in response to P. syringae, PRR2 induces the production of SA and the accumulation of the defence-related protein PR1. Moreover, PRR2 overexpressing lines exhibit an enhanced production of camalexin, a phytoalexin that confers enhanced resistance against pathogens. Together, these data reveal the importance of PRR2 in plant immune responses against P. syringae and suggest a novel function for this particular plant specific transcription factor in plant physiology.
Oleanolic Acid Enhances Mesenchymal Stromal Cell Osteogenic Potential by Inhibition of Notch Signaling
Authors: Shu Bing , Zhao Yongjian , Wang Yongjun , Wang Guangxi , Shang Xifu , Britt Michael , Olmedo Margaret , Chelly Marjorie , Morandi Massimo Max , Barton Shane , Dong Yufeng
Published: July 2017

Oleanolic acid (OA), a pentacyclic triterpenoid, has been shown to modulate multiple signaling pathways in a variety of cell linages. But the mechanisms underlying OA-mediated mesenchymal stromal cell (MSC) osteogenic differentiation are not known. In this study, we examined effects of OA on cell viability, osteogenic differentiation in MSCs, and the involvement of Notch and BMP signaling. OA induced bone marrow derived MSC differentiation towards osteoprogenitor cells and inhibited Notch signaling in a dose dependent manner. Constitutive activation of Notch signaling fully blocked OA induced MSC osteogenic differentiation. The expression level of early osteogenic marker genes, ALP, Runx2, and type I collagen, which play a critical role in MSC to osteoblast transition and servers as a downstream target of BMP signaling, was significantly induced by OA. Furthermore, BMP2 mediated MSC osteogenic differentiation was significantly enhance by OA treatment, indicating a synergistic effect between BMP2 and OA. Our results suggest that OA is a promising bioactive agent for bone tissue regeneration, and inhibition of Notch signaling is required for its osteogenic effects on MSCs.
Hybrid organic-inorganic coatings via electron transfer behaviour
Authors: Zoubi Wail Al , Min Ji Hoon , Ko Young Gun
Published: July 2017

A novel method to functionalize the surface of inorganic coating by growing organic coating has been investigated based on microstructural interpretation, electrochemical assessment, and quantum chemical analysis. For this purpose, inorganic coating with magnesium aluminate, magnesium oxide, and titanium dioxide was prepared on magnesium alloy via plasma electrolytic oxidation (PEO), and, then, subsequent dip-coating method was used to tailor organic coating using diethyl-5-hydroxyisophthalate (DEIP) as organic molecules. The incorporation of TiO2 particles worked as a sealing agent to block the micro-defects which resulted mainly from the intense plasma sparks during PEO. In addition, such incorporation played an important role in enhancing the adhesion between inorganic and organic coatings. The use of DEIP as organic corrosion inhibitor resulted in a significant decrease in porosity of inorganic coating. Quantum chemical calculation was used to clarify the corrosion inhibition mechanism which was activated by introduction of DEIP. Thus, the electrochemical analysis based on potentiodynamic polarization and impedance spectroscopy tests in 3.5 wt% NaCl solution suggested that corrosion resistance of magnesium alloy sample was enhanced significantly due to a synergistic effect arising from the hybrid inorganic and organic coatings. This phenomenon was explained in relation to electron transfer behaviour between inorganic and organic coatings.
Comment on “Laser produced plasma diagnosis of carcinogenic heavy metals in gallstones” by M. A. Gondal, M. A. Shemis, A. A. I. Khalil, M. M. Nasr and B. Gondal, JAAS, 2016, 31, 506
Authors: Popov Andrey M. , Labutin Timur A. , Zaytsev Sergey M.
Published: July 2017

The selection of analytical lines to determine Pb, Cr, Cd, Hg, and Ni in gallstones in the recently published paper with the above title is criticized.
Three-dimensional microtissues as an in vitro model for personalized radiation therapy
Authors: Qiu Yuting , Ning Dandan , Zhang Peipei , Curly Stephanie , Qiao Yong , Ma Liyuan , Su Ming
Published: July 2017

This paper describes the use of 3D microtissues as an intermediate model between the 2D cell culture and the animal model to assess radiation-induced cellular and DNA damage in the context of personalized radiation therapy.
Phenol-selective mass spectrometric analysis of jet fuel
Authors: Zhu Haoxuan , Janusson Eric , Luo Jingwei , Piers James , Islam Farhana , McGarvey G. Bryce , Oliver Allen G. , Granot Ori , McIndoe J. Scott
Published: July 2017

A bromobenzyl charged tag selectively reacts with key contaminants in jet fuel to provide charged ethers that are readily detectable by ESI-MS.