bluuebunny/crossref_metadata_2025 huggingface dataset:
TRATAMENTOS MEDICAMENTOSOS DE CRIANÇAS COM TDAH: UMA REVISÃO SISTEMÁTICA
Authors: Mariano Paulo Luy Alencar Vieira , Pereira Adriano Pontes , Soares Jara Couceiro , Barros Rogélio Rocha
Published: October 2024

A Ritalina, enquanto monoterapia no tratamento de crianças com Transtorno do Déficit de Atenção e Hiperatividade (TDAH), ocupa um papel central na psicofarmacologia pediátrica. Este estudo visa contextualizar essa abordagem dentro de um panorama abrangente do tratamento do TDAH, por meio de uma revisão sistemática dos desfechos terapêuticos associados ao uso do Metilfenidato. A pesquisa foi realizada utilizando bases de dados como PubMed, Scielo e o Portal Regional da BVS, aplicando descritores em português e inglês relacionados ao TDAH em crianças e ao uso do Metilfenidato. Foram incluídos artigos publicados entre 2018 e 2024, com a devida consideração para estudos relevantes anteriores a esse período. Os resultados da revisão sistemática indicam que o Metilfenidato, quando administrado na infância, traz benefícios significativos no desenvolvimento psicossocial, especialmente na melhora da atenção e no controle dos impulsos. No entanto, destaca-se a importância de uma abordagem cautelosa devido aos possíveis efeitos adversos no sistema nervoso central (SNC), como aumento da ansiedade, elevação da frequência cardíaca e potenciais impactos sobre os astrócitos. Em conclusão, o uso do Metilfenidato como monoterapia tem se mostrado eficaz no manejo dos sintomas do TDAH, e embora efeitos colaterais estejam presentes, estes não superam os benefícios substanciais oferecidos pelo tratamento.
A PERCEPÇÃO DA MULHER SOBRE OS DESAFIOS DO ALEITAMENTO MATERNO
Authors:   Lorena Campos Santos, Dias Eslayne Lopes , Mendes Sarah Bispo , Martins Sandy Hevellyn Alencar
Published: October 2024

Objetivo: Identificar os desafios para as mães durante o período do  aleitamento materno. Metodologia: Trata-se de revisão integrativa, com  produções que utilizaram abordagem qualitativa em estudos da temática da  Percepção da mulher sobre os desafios do aleitamento materno exclusivo na  gestação e o papel do enfermeiro na educação em saúde, trabalhando com  estratégias e ações educativas, com objetivos de minimizar o desmame  precoce. Critérios de inclusão publicados no idioma português, publicados no ano de 2010 a 2021. Resultados: Estudos mostram que o desmame precoce é  corriqueiro e está associado a fatores como problemas mamários, crenças,  mitos e medos. Considerações Finais: Compreende-se que o enfermeiro tem  participação fundamental no processo de acompanhamento de binômios.  Assim, com a educação em saúde e a promoção do aleitamento materno  exclusivo, diminui as ocorrências de episódios de doenças relacionadas à saúde do bebê. Enfatizando que, é importante conhecer as principais causas e  manifestações da amamentação ineficaz permitindo subsidiar as ações de  enfermagem de forma mais direcionada.
IMPACTO DOS COMPOSTOS BIOATIVOS DA UVA, COM ÊNFASE NO RESVERATROL, NA REDUÇÃO DA PRESSÃO ARTERIAL: UMA REVISÃO DE LITERATURA
Authors: Nunes Filipe Bonfim , Ferreira Alessandra de Freitas , Cotinho Bruno Flexa Quaresma , Silva Shirley Helena dos Santos Henriques da , Ferreira Luis Eduardo Batista , Frade Luan Felipe da Silva , Oliveira Robert Davis Souza de , Silva José Willian de Almeida , Cavalcante Patrícia Alves de Mendonça , Nascimento Luzimere Pires do
Published: October 2024

The study aimed to analyze the main studies that address the impacts of grape bioactive compounds, with an emphasis on resveratrol, on reducing blood pressure. This is a literature review, which was carried out based on the definition of the research question, inclusion and exclusion criteria, search strategies, and selection of studies. 40 articles were selected from 230 identified, after assessing their methodological quality. Data analysis focused on the mechanisms of action of resveratrol, the effectiveness of supplementation methods, and its clinical perspectives. The study highlighted the potential of resveratrol, a grape bioactive compound, in reducing blood pressure, acting as an adjuvant in the treatment of hypertension. It promotes vasodilation, reduces oxidative stress, and improves cardiovascular health. Its effectiveness, however, depends on dosage and bioavailability. Despite advances, more clinical studies are needed to standardize its use and investigate interactions with medications. Continued research can consolidate resveratrol in integrative health strategies.
Performance and Emission Analysis of Ethanol-Petrol Blends in Single-Cylinder Engines: A Sustainability Perspective
Authors: Gurav Pratik
Published: October 2024

This study investigates the blending of ethanol and petrol in the Indian subcontinent, addressing the urgent need for sustainable fuel alternatives amid rising energy demands and environmental concerns. Ethanol, as a renewable biofuel, presents a viable option for enhancing the efficiency of internal combustion engines while reducing harmful emissions. Given the significant air pollution issues prevalent in urban areas of India, optimizing fuel blends can substantially improve engine performance and contribute to cleaner air. The primary objectives of this research include analyzing the impact of different ethanol-petrol blends on the performance of a single-cylinder petrol engine, assessing key performance metrics such as brake power, Indicated Mean Effective Pressure (IMEP), Brake Mean Effective Pressure (BMEP), and Brake Thermal Efficiency (BTHE). Emission analysis was conducted to measure hydrocarbons (HC), carbon monoxide (CO), carbon dioxide (CO₂), oxygen (O₂), and nitrogen oxides (NOₓ) across various blends, quantifying the environmental impact of each. Grey Relation Analysis (GRA) was employed to rank the fuel blends based on their performance and emissions, helping identify the most effective blend for optimal engine operation. Additionally, the sustainability of ethanol blending as an alternative fuel solution in the Indian automotive sector was assessed. The study recorded key performance indicators and emissions using standardized techniques, with GRA calculating Grey relational coefficients to measure the degree of similarity between observed data and desired outcomes. This approach facilitated the ranking of blends, ultimately identifying the optimal ethanol-petrol blend. The findings revealed significant variations in both performance and emissions across different blends, indicating that ethanol blending holds substantial promise as a sustainable solution for the Indian automotive sector. These insights align with recent studies on the benefits of biofuels in enhancing engine performance and reducing emissions, providing valuable guidance for optimizing ethanol-petrol blends to improve sustainability in energy consumption.
Las especies del subgénero Atomaria Stephens, 1829 (Coleoptera, Cryptophagidae) de la China continental y descripción de una nueva especie
Authors: Otero José Carlos
Published: October 2024

The article provides a list of the 47 species of subgenre Atomaria Stephens, 1829 (Coleoptera, Cryptophagidae) recorded in China and adjacent regions (Mongolia, Russian Far East, Nepal, Bhutan, northeastern part of India, Kyrgyzstan, Uzbekistan, Kazakhstan, Turkmenistan, Tajikistan, Japan, N. Korea, N. Thailand). Atomaria (Atomaria) derougemonti sp. nov. is described.
“A hot topic”: TTR amyloidosis gets its moment to shine at ESC 2024
Authors: von Riedemann Sarah
Published: October 2024

n transthyretin amyloidosis (ATTR), the liver produces an abnormal transthyretin (TTR) protein, which aggregates into amyloid fibrils that accumulate in peripheral nerves and the heart. Over time, this can lead to progressive cardiomyopathy (ATTR-CM), heart failure (HF), and premature mortality. At the recent meeting of the ESC there was much excitement and enthusiasm about ATTR-CM, largely inspired by the HELIOS-B trial of vutrisiran, an RNA interference (RNAi) agent that, if approved, would become only the second medication indicated for ATTR-CM. This report reviews the most relevant insights from the meeting into the diagnosis and management of ATTR-CM, with expert commentary on their relevance and implications for Canadian clinicians.
HOW CASSIA ALATA L AFFECTS BLOOD GLUCOSE LEVELS IN DIABETES MELLITUS PATIENTS?
Authors: Sabrina Salsabilah  , Mustika Sari  , Muthmainnah J. Abdullah  , Said Kurniati  , Emy Izzati  , I Putu Sudayasa
Published: October 2024

Candle bush (Cassia alata L.) are plants that are rich in antioxidants. There have been many studies that discuss the efficacy of Candle bush (Cassia alata L.) in skin diseases, but there is still less research that discusses the potential of Candle bush leaves as antidiabetics. Therefore, researchers want to test the effectiveness of Candle bush leaves (Cassia alata L.) in improving blood glucose levels (antidiabetic) using experimental animals (Rattus norvegicus strain wistar) induced with Streptozotosin (STZ). Candle bush leaf extract (Cassia alata L.) was made with maceration method using ethanol 70% solution. Experimental animals were divided into 6 groups. Group 1,2, and 3 are the control group, Group 4,5, and 6 are the groups that are given the extract with different doses each. This study proves that extract of Candle bush leaf (Cassia alata L.) can improve blood glucose levels of the experimental animals (Rattus norvegicus strain wistar).
HUBUNGAN KOMUNIKASI TERAPEUTIK PERAWAT DENGAN KEPUASAN PASIEN DI IGD RSU GMIM BETHESDA TOMOHON
Authors: Mandagi Autry , Ponamon Jolie.F. , Wenas Melinda
Published: October 2024

Komunikasi terapeutik memegang peranan penting dalam membangun pemahaman pasien tentang pelayanan kesehatan yang akan diterima, Komunikasi ini penting karena masalah utama pasien adalah mengalami kecemasan akan penyakit dan tindakan-tindakan kesehatan yang akan diterima. Penelitian ini bertujuan untuk mengetahui hubungan komunikasi terapeutik dengan kepuasan pasien di IGD RSU GMIM Bethesda Tomohon. Penelitin di lakukan pada bulan Maret 2022. Rancangan penelitian yang digunakan deskriptif dengan pendekatan Cross Sectionel. Populasi berjumlah 281 orang. Teknik sampling yaitu purposif sampling dengan sampel 166 responden. Istrumen penenelitian menggunakan kuisioner. Analisa yang digunakan analisa univariat (jenis kelamin, umur, jenis pekerjaan) dan analisa bivariat menggunakan uji chi squer. Hasil penelilitian didapati komunikasi dengan kepuasan p= 0,004 < α 0,05 yang berarti terdapat hubungan komunikasi terapeutik dengan kepuasan pasien atau Ha di terima Ho do tolak. Kesimpulan dari penelitian ini terdapat hubungan komunikasi terapeutik dengan kepuasan pasien di IGD RSU GMIM Bethesda Tomohon
L’AMPLIFICATION: ESQUISSE D’ANALYSE COMPARÉE DE L’ENGAGEMENT CORPOREL DES BASSISTES ET DES GUITARISTES
Authors: Grün Laurent
Published: October 2024

L’électrification de la guitare et de la contrebasse inaugure une lutherie originale. La « six-cordes » devient fine, au corps compact, avec l’émergence des modèles solid body. La « quatre-cordes », elle, est une révolution par rapport à la contrebasse en copiant la structure de la guitare et en modifiant la tenue de l’instrument du « posé » au « porté ». Pour autant, si l’électrification des deux instruments conduit à une rationalisation unique autour de la guitare électrique à « six cordes » ou « quatre cordes », cela n’a pas les mêmes conséquences en matière de motricité, corporéité et jeu. Phénoménologiquement, évoquer la guitare électrique renvoie au rapport fusionnel perceptible entre l’instrument et le musicien, qui exhibe son corps et sa virilité à travers un usage phallique du manche. Au contraire, l’électrification de la contrebasse, qui induit pourtant davantage de conséquences au plan formel, n’empêche pas la conservation d’une retenue et d’un échange sensuel entre le musicien et l’instrument, quels que soient la culture et le style. La discrétion demeure la norme du bassiste, l’exhibition celle du « six-cordiste ». Notre projet visera à identifier les différences et les similitudes dans l’interface gestuelle, l’engagement du corps, la motricité au travers de l’utilisation de la basse et de la guitare électriques. Notre appartenance aux STAPS (sciences et techniques des activités physiques et sportives) incite à l’analyse de la mise en jeu du corps, du mouvement et de la motricité des objets d’investigation, même si nous n’avons pas affaire à un exercice physique. Notre « chapelle scientifique » confère, nous semble-t-il, une certaine légitimité à nous intéresser à ce « corps à corps » entre instruments électriques et musiciens.
LINK WRAY, À LA RECHERCHE DU SON SALE ET SAUVAGE
Authors: Gilles Guillaume
Published: October 2024

Si la guitare électrique est devenue l’instrument symbolique du rock à partir des années 1960, le timbre saturé auquel on l’associe communément dans ce genre musical n’a pas attendu les déluges de larsens de Jimi Hendrix, de Jeff Beck ou de Jimmy Page pour s’imposer dans le rock. Plus tôt déjà, dans le rock’n’roll américain, des guitaristes comme Chuck Berry, Grady Martin, Mickey Baker se sont intéressés aux possibilités sonores et expressives de la distorsion. Par un travail d’expérimentation sur la surcharge de signal, ces guitaristes se sont créé de toutes pièces une identité sonore au cœur des timbres saturés. Ce texte s’attache plus directement à retracer la quête du son sale et sauvage de Link Wray, exemple emblématique des changements survenus entre le rock’n’roll et le rock anglais qui profita de la nouvelle expressivité offerte par ces timbres inouïs. Pour ce faire, nous utiliserons une méthode d’analyse comparative s’articulant autour d’approches complémentaires, de la phénoménologie la plus élémentaire à une lecture sociale des changements musicaux. Après un rapide passage en revue des effets, timbres et modes de jeu de guitaristes électriques des années 1950 (« slapback » de Scotty Moore, trémolo de Bo Diddley, « fuzz » de Grady Martin…), nous observerons comment Link Wray est parvenu à anticiper les nouvelles possibilités offertes par la guitare électrique: la recherche du son saturé, le développement du power chord par lesquels Wray illustrait sa posture à l’égard de la dureté du quotidien. Nous verrons finalement par l’analyse de Rumble comment la guitare électrique a pu devenir le symbole d’une certaine sauvagerie du rock, à travers le caractère agressif, menaçant, indomptable dont elle a pu donner, tour à tour ou simultanément, l’image.
Sistem Pendukung Keputusan Penerimaan Karyawan Menggunakan Metode Weighted Product (WP) Pada BMT NU Cabang Suboh
Authors: Al Halimi Moh. Riqza , Ridla Muhammad Ali , Susanto Adi
Published: October 2024

Karyawan merupakan salah satu aset perusahaan yang paling berharga. Pegawai menjadi SDM yang sangat penting bagi perusahaan.  Untuk memperoleh pegawai yang sesuai dengan kebutuhan kantor maka dilakukan rekrutmen. KSPP Syariah BMT NU merupakan Lembaga keuangan Koperasi Simpan Pinjam dan Pembiayaan yang berbasis Syariah. Proses seleksi pegawai baru masih dilakukan secara manual dengan sistem administrasi sehingga memerlukan waktu yang cukup lama untuk menenrukan siapa yang akan diterima. Penelitian ini menggunakan metode Weighted Product (WP) bertujuan untuk bisa membantu dalam proses penerimaan karyawan. Dalam proses rekrutmen karyawan digunakan enam kriteria yaitu Kelengkapan Berkas Lamaran, Pendidikan Terakhir, Pengalaman Kerja, Usia, Tes Kemampuan (Skill) dan Tes Interview. Hasil penelitian ini memutuskan bahwa nilai tertinggi dengan peringkat pertama yaitu Alternatif 6 dengan perolehan nilai 0,1628.  Adapun tujuan penelitian ini diharapkan bisa memudahkan dan mempercepat saat memilih pegawai yang paling memenuhi kebutuhan kantor.
Six Sigma untuk Peningkatan Kualitas Sajian Makanan Dine-In: Studi Kasus Usaha Makanan dan Minuman di Indonesia
Authors: Destyanto Arry Rahmawan , Nurimannisa Melinda
Published: October 2024

Penelitian ini mengusulkan implementasi kerangka kerja Six Sigma di industri non-manufaktur, terutama industri makanan dan minuman melalui studi kasus nyata untuk meningkatkan kualitas sajian makanan di tempat (Dine-In). Pendekatan Define, Measure, Analyze, Improve, dan Control (DMAIC) Six Sigma diterapkan untuk mengidentifikasi akar penyebab masalah dan merumuskan solusi efektif untuk peningkatan kualitas sajian. Hasil implementasi menunjukkan peningkatan signifikan dalam kualitas sajian Dine-In dengan mengurangi kecacatan sajian sebesar 62,5% dibandingkan kondisi sebelum implementasi Six Sigma. Studi ini menjadi yang pertama di literatur dalam memberikan panduan praktis untuk menerapkan Six Sigma dalam konteks non-manufaktur, khususnya untuk meningkatkan kualitas sajian Dine-In pada industri makanan dan minuman yang dapat dijadikan rujukan bagi banyak pelaku industri terkait di Indonesia.
Analisis Pengendalian Persediaan Bahan Kain di UMKM IBS Menggunakan Metode Peramalan dan EOQ
Authors: Imansyah Teguh , Andesta Deny
Published: October 2024

UMKM IBS merupakan bisnis di bidang home industry manufaktur. Produk yang dibuat yaitu sofa bayi, perlengkapan tidur bayi, dll. Dengan memakai bahan baku kain utama yaitu tetron, kapas, dan knitting. Namun, pembelian yang berlebihan dan kekurangan bahan menyebabkan stok beberapa bahan kain, yang membuat sulit untuk memprediksi jumlah produksi di masa mendatang. Penelitian ini bertujuan untuk mengetahui jumlah pemesanan perusahaan dan biaya persedian total dengan metode peramalan dan jumlah pemesanan ekonomis. Dalam perhitungan TIC didapatkan hasil TIC EOQ memiliki biaya ekonomis untuk bahan kain tetron sebesar Rp. 10.970 sedangkan TIC Perusahaan sebesar Rp. 51.387. Hasil peramalan bahan kain tetron sebesar 530/m, kapas sebesar 523/m, dan knitting sebesar 514/m. Sedangkan hasil perhitungan EOQ diperoleh nilai bahan kain tetron sebesar 50/m, kapas sebesar 50/m, dan knitting sebesar 50/m.
Analisis Risiko Pada Proses Produksi Dengan Menerapkan Metode House of Risk, AHP dan Pendekatan SCOR Pada PT XYZ
Authors: Ghozali Mohammad Bima , Hidayat Hidayat , Negoro Yanuar Pandu
Published: October 2024

PT XYZ merupakan perusahaan jasa yang bergerak di bidang pengolahan air. Perusahaan ini mengalami banyak masalah dalam proses produksi dan tidak ada rencana mitigasi yang terstruktur. Tujuan dari penelitian ini adalah untuk mengetahui risiko-risiko yang diperkirakan ada atau sudah ada dan memberikan rencana mitigasi. Penelitian ini menggunakan pendekatan SCOR untuk pemetaan dan metode House Of Risk untuk menganalisis dan memberikan mitigasi risiko. Setelah itu dilakukan analisis risiko pada House Of Risk fase 1 yang menghasilkan 35 Risk event & 30 Risk Agent dengan 5 hasil prioritas risiko yang didapat dari estimasi ARP diantaranya A16, A28, A26, A10, A18, sedangkan untuk preventive action yang didapat dari estimasi AHP sebanyak 8 yaitu PA1 dengan nilai 0, PA2 dengan nilai 26, PA3 dengan nilai 0,20, PA2 dengan nilai 0,17, PA4 dengan nilai 0,13, PA5 dengan nilai 0,12, PA6 dengan nilai 0,08, PA7 dengan nilai 0,03, PA8 dengan nilai 0,02.
Metode Klasifikasi Tingkat Kematangan Buah dan Sayuran : Tinjauan Sistematis
Authors: Saktriawindarta Rama , Kusrini Kusrini
Published: October 2024

Klasifikasi kematangan buah dan sayuran penting untuk memastikan kualitas produk dalam industri pertanian. Tujuan penelitian ini adalah mengoptimalkan waktu panen dan distribusi dengan metode deep learning dan machine learning. Sistematic Literatur Review (SLR) digunakan untuk mengidentifikasi metode dan model klasifikasi yang efektif. Metode yang dominan adalah image processing (65%), diikuti machine learning (50%) dan deep learning (42.5%). Model seperti CNN, AlexNet, dan ResNet-50 menunjukkan akurasi tinggi. Evaluasi kinerja menggunakan metrik seperti akurasi, precision, recall, dan F1-score. Untuk meningkatkan akurasi, penelitian di masa depan disarankan untuk mengumpulkan dataset lebih beragam dan menggunakan metode hybrid. Pengembangan infrastruktur komputasi dan pelatihan tenaga kerja juga diperlukan untuk penerapan teknologi ini dalam industri pertanian.
Komparasi Algoritma Machine Learning Untuk Menganalisis Sentimen Ulasan Pada Aplikasi Digital Korlantas Polri
Authors: Delimasari Siti , Kusrini Kusrini
Published: October 2024

Aplikasi Digital Korlantas Polri merupakan aplikasi mobile yang memberikan kemudahan bagi masyarakat dalam memperpanjang SIM. Analisis sentimen terhadap ulasan pengguna membantu Korlantas Polri mengidentifikasi persepsi publik terhadap layanan yang diberikan. Penelitian ini bertujuan mengevaluasi lima algoritma machine learning mana yang paling baik performanya dari Support Vector Machine (SVM), Naive Bayes, Random Forest, K-Nearest Neighbors (KNN), dan Logistic Regression dalam proses analisis sentimen. Evaluasi dilakukan dengan mengukur akurasi, presisi, recall dan F1 measure. Terdapat 10.000 ulasan diberi label dengan validasi ahli bahasa, diproses ulang, diberi bobot kata setelah data dikumpulkan. Teknik over-sampling minoritas sintetis (SMOTE) diterapkan sebelum pembagian data untuk pelatihan dan pengujian. Hasil evaluasi menunjukkan Random Forest dan SVM melakukannya dengan paling baik. Random Forest memiliki akurasi  90,77%, recall 90,77%, dan nilai F1 tertinggi yaitu 90,79%. SVM memiliki presisi tertinggi dengan 91,14% di antara algoritma lainnya, yang menunjukkan potensi besar kedua algoritma ini menganalisis sentimen ulasan aplikasi digital Korlantas Polri.
Peningkatan Pelayanan Data Keuangan Siswa Melalui Sistem Informasi Keuangan Monte Carmelo (SIMOKAR) dengan Metode Extreme Programming
Authors: Wara Febriyanti Alwisye , Muda Henderikus Basilius Nong , Ermilinda Lindiana , Chandra Conchita Junita , Mado Theresia Wihelmina , Lodan Maria Wihelmina
Published: October 2024

SMAK Santa Maria Monte Carmelo, sebuah sekolah di bawah Yayasan Santa Maria Karmel di Wairklau-Maumere, saat ini masih menggunakan pencatatan laporan keuangan siswa secara manual dalam sebuah buku keuangan. Sistem ini menghadapi kendala seperti ketidakakuratan laporan keuangan dan seringnya kehilangan data, yang mengakibatkan laporan keuangan menjadi tidak valid. Untuk mengatasi masalah tersebut, penelitian ini bertujuan untuk mengembangkan sebuah aplikasi yang membantu bendahara sekolah dalam mencatat pembayaran secara cepat dan akurat. Aplikasi yang dirancang yaitu Sistem Informasi Keuangan Monte Carmelo (SIMOKAR), menggunakan bahasa pemrograman Visual Basic (VB.Net) dan MySQL sebagai penyimpanan data. Dalam pengembangannya, aplikasi ini mengadopsi metode Extreme Programming untuk memastikan keefektifan dan keakuratan dalam mencatat pembayaran keuangan siswa. Hasil penelitian menunjukkan bahwa sistem ini berhasil mengimplementasikan pencatatan pembayaran secara otomatis dan efisien sesuai dengan pengujian dari black box testing. Aplikasi SIMOKAR mendapat tingkat kepuasan pengguna sebesar 90%.
Measuring IT Governance in ERP Systems: a COBIT 2019 Evaluation of SAP Material Management Module
Authors: Hariman Putri Aulia , Fianty Melissa Indah
Published: October 2024

Information technology systems such as Enterprise Resource Planning (ERP) are crucial for companies to meet their business needs. However, as companies grow, challenges arise, particularly in the Material Management (MM) module within SAP. This study aims to identify issues such as data inaccuracies and material duplication during the interfacing process with SAP MM and assess IT governance capabilities using the COBIT 2019 framework. The methods used include interviews and literature review, focusing on the domains of APO12 (Managed Risk), BAI03 (Managed Solutions Identification & Build), and DSS03 (Managed Problems). The results show that APO12 reached capability level 4, while BAI03 and DSS03 are at level 3, with a target of level 4. Recommendations include enhancing proactive system design evaluation, developing systematic procedures to address data issues, and implementing permanent solutions to prevent data duplication and inaccuracies during interfacing.
Political Participation for Social Inclusion of Internally Displaced Persons
Authors: Iorbo Rita , Sahni Sanjeev P. , Bhatnagar Tithi , Andzenge Dick T.
Published: October 2024

Internally displaced persons (IDPs) have suffered multiple disadvantages and experienced social exclusion due to involuntary movement to new communities where they struggle to find opportunities for social inclusion. This study examined the political dimensions of social inclusion which focus on engaging IDPs in decision-making on issues that concern them. The study used the democratic participatory theory as a framework for political participation through decision-making. The qualitative exploratory study used in-depth interviews to collect data from twelve IDPs, comprising five females and seven males, who were resident in Benue State, Nigeria. Findings show that government does not incorporate the decisions and choices of IDPs when designing humanitarian measures for protection and assistance; IDPs are not communicated with on issues of interest to them; and IPDs lack sustainable opportunities for interaction with host communities. The study concludes that not incorporating the decisions of IDPs in issues of interest to them creates a culture of humanitarian aid dependency. The study recommends democratising solutions by using grassroots bottom-up measures for sustainable social inclusion of IDPs where solutions emerge from IDPs who are the end beneficiaries of the interventions. A discussion of the study findings is followed by concluding recommendations.
Reprograming gene expression in hibernating C. elegans involves the IRE-1/XBP-1 pathway
Authors: Engelfriet Melanie L , Guo Yanwu , Arnold Andreas , Valen Eivind , Ciosk Rafal
Published: October 2024

In the wild, many animals respond to cold temperatures by entering hibernation. In the clinic, controlled cooling is used in transplantation and emergency medicine. Yet, the molecular mechanisms that the cells use to survive cold remain largely unexplored. One aspect of cold adaptation is a global downregulation of protein synthesis. Studying it in the nematode
Caenorhabditis elegans
, we find that the translation of most mRNAs continues in the cold, albeit at a slower rate, and propose that cold-specific gene expression is regulated primarily at the transcription level. Moreover, we show that the transcription of some cold-induced genes reflects the activation of unfolded protein response (UPR) mediated by the conserved IRE-1/XBP-1 signaling pathway. Our results suggest that the activation of this pathway stems from cold-induced perturbations in proteins and lipids in the endoplasmic reticulum and that its activation is beneficial for cold survival.
LAPTM4B Alleviates Pulmonary Fibrosis by Enhancing NEDD4L-Mediated TGF-β Signaling Suppression
Authors: Xu Kai , Pan Xiaoyue , Lian Hui , Wang Yaxuan , Wan Ruyan , Li Zhongzheng , Pan Xin , Li Yajun , Yang Juntang , Rosas Ivan , Wang Lan , Yu Guoying
Published: October 2024

Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive lung disease with fatal outcome and a poorly understood pathogenesis. The lysosomal protein transmembrane 4 beta (LAPTM4B), a multi-transmembrane endo-lysosomal membrane protein, has been implicated in the pathogenesis of several diseases. However, its involvement in IPF remains unexplored. This study aimed to investigate the role of LAPTM4B in lung fibrosis and elucidate its underlying mechanisms. The results showed that LAPTM4B was significantly reduced in IPF and mouse fibrotic lungs. In vivo studies showed that the deficiency of LAPTM4B exacerbated bleomycin-induced lung fibrosis, while the restoration of LAPTM4B alleviated fibrosis. Mechanistically, LAPTM4B recruits the NEDD4 like E3 ubiquitin protein ligase (NEDD4L) to endosomes, leading to increased ubiquitin-mediated proteasomal degradation of TGFRB2 and active SMAD2/3, thereby blocking the TGF-β/SMAD signaling pathway. Overall, our data provided a novel insight for a deeper understanding of the pathogenesis of IPF, supporting the therapeutic potential of restoration of LAPTM4B as a promising therapeutic approach for the treatment of pulmonary fibrosis.
Protein-Induced Membrane Strain Drives Supercomplex Formation
Authors: Pöverlein Maximilian C , Jussupow Alexander , Kim Hyunho , Kaila Ville RI
Published: October 2024

Mitochondrial membranes harbor the electron transport chain (ETC) that powers oxidative phosphorylation (OXPHOS) and drives the synthesis of ATP. Yet, under physiological conditions, the OXPHOS proteins operate as higher-order supercomplex (SC) assemblies, although their functional role remains poorly understood and much debated. By combining large-scale atomistic and coarse-grained molecular simulations with analysis of cryo-electron microscopic data and statistical as well as kinetic models, we show here that the formation of the mammalian I/III
2
supercomplex reduces the molecular strain of inner mitochondrial membranes by altering the local membrane thickness, and leading to an accumulation of both cardiolipin and quinone around specific regions of the SC. We find that the SC assembly also affects the global motion of the individual ETC proteins with possible functional consequences. On a general level, our findings suggest that molecular crowding and entropic effects provide a thermodynamic driving force for the SC formation, with a possible flux enhancement in crowded biological membranes under constrained respiratory conditions.
Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
Authors: Matsuo-Takasaki Mami , Kambayashi Sho , Hemmi Yasuko , Wakabayashi Tamami , Shimizu Tomoya , An Yuri , Ito Hidenori , Takeuchi Kazuhiro , Ibuki Masato , Kawashima Terasu , Masayasu Rio , Suzuki Manami , Nishishita Naoki , Kawai Yoshikazu , Umekage Masafumi , Kato Tomoaki M , Noguchi Michiya , Nakade Koji , Nakamura Yukio , Nakaishi Tomoyuki , Tsukahara Masayoshi , Hayashi Yohei
Published: October 2024

Human induced pluripotent stem cells (hiPSCs) are promising resources for producing various types of tissues in regenerative medicine; however, the improvement in a scalable culture system that can precisely control the cellular status of hiPSCs is needed. Utilizing suspension culture without microcarriers or special materials allows for massive production, automation, cost-effectiveness, and safety assurance in industrialized regenerative medicine. Here, we found that hiPSCs cultured in suspension conditions with continuous agitation without any microcarriers or extracellular matrix components were more prone to spontaneous differentiation than those cultured in conventional adherent conditions. Adding PKCβ and Wnt signaling pathway inhibitors in the suspension conditions suppressed the spontaneous differentiation of hiPSCs into ectoderm and mesendoderm, respectively. In these conditions, we successfully completed the culture processes of hiPSCs including the generation of hiPSCs from peripheral blood mononuclear cells with the expansion of bulk population and single-cell sorted clones, long-term culture with robust self-renewal characteristics, single-cell cloning, direct cryopreservation from suspension culture and their successful recovery, and efficient mass production of a clinical-grade hiPSC line. Our results demonstrate that precise control of the cellular status in suspension culture conditions paves the way for their stable and automated clinical application.
Heat Shock Factor 1 forms nuclear condensates and restructures the yeast genome before activating target genes
Authors: Rubio Linda S , Mohajan Suman , Gross David S
Published: October 2024

In insects and mammals, 3D genome topology has been linked to transcriptional states yet whether this link holds for other eukaryotes is unclear. Using both ligation proximity and fluorescence microscopy assays, we show that in Saccharomyces cerevisiae, Heat Shock Response (HSR) genes dispersed across multiple chromosomes and under the control of Heat Shock Factor (Hsf1) rapidly reposition in cells exposed to acute ethanol stress and engage in concerted, Hsf1-dependent intergenic interactions. Accompanying 3D genome reconfiguration is equally rapid formation of Hsf1-containing condensates. However, in contrast to the transience of Hsf1-driven intergenic interactions that peak within 10–20 min and dissipate within 1 hr in the presence of 8.5% (v/v) ethanol, transcriptional condensates are stably maintained for hours. Moreover, under the same conditions, Pol II occupancy of HSR genes, chromatin remodeling, and RNA expression are detectable only later in the response and peak much later (>1 hr). This contrasts with the coordinate response of HSR genes to thermal stress (39°C) where Pol II occupancy, transcription, histone eviction, intergenic interactions, and formation of Hsf1 condensates are all rapid yet transient (peak within 2.5–10 min and dissipate within 1 hr). Therefore, Hsf1 forms condensates, restructures the genome and transcriptionally activates HSR genes in response to both forms of proteotoxic stress but does so with strikingly different kinetics. In cells subjected to ethanol stress, Hsf1 forms condensates and repositions target genes before transcriptionally activating them.
The E3 ubiquitin ligase RNF220 maintains hindbrain Hox expression patterns through regulation of WDR5 stability
Authors: Wang Huishan , Liu Xingyan , Liu Yamin , Yang Chencheng , Ye Yaxin , Yu Xiaomei , Sheng Nengyin , Zhang Shihua , Mao Bingyu , Ma Pengcheng
Published: October 2024

The spatial and temporal linear expression of
Hox
genes establishes a regional
Hox
code, which is crucial for the antero-posterior (A-P) patterning, segmentation, and neuronal circuit development of the hindbrain. RNF220, an E3 ubiquitin ligase, is widely involved in neural development
via
targeting of multiple substrates. Here, we found that the expression of
Hox
genes in the pons was markedly up-regulated at the late developmental stage (post-embryonic day E15.5) in

Rnf220
−/−

and

Rnf220
+/−

mouse embryos. Single-nucleus RNA-seq analysis revealed different
Hox
de-repression profiles in different groups of neurons, including the pontine nuclei (PN). The
Hox
pattern was disrupted and the neural circuits were affected in the PN of

Rnf220
+/−

mice. We showed that this phenomenon was mediated by WDR5, a key component of the TrxG complex, which can be polyubiquitinated and degraded by RNF220. Intrauterine injection of WDR5 inhibitor (WDR5-IN-4) and genetic ablation of
Wdr5
in

Rnf220
+/−

mice largely recovered the de-repressed
Hox
expression pattern in the hindbrain. In P19 embryonal carcinoma cells, the retinoic acid induced
Hox
expression was further stimulated by
Rnf220
knockdown, which can also be rescued by
Wdr5
knockdown. In short, our data suggest a new role of RNF220/WDR5 in
Hox
pattern maintenance and pons development in mice.
Ih block reveals separation of timescales in pyloric rhythm response to temperature changes in Cancer borealis
Authors: Schapiro Kyra , Rittenberg JD , Kenngott Max , Marder Eve
Published: October 2024

Motor systems operate over a range of frequencies and relative timing (phase). We studied the role of the hyperpolarization-activated inward current (Ih) in regulating these features in the pyloric rhythm of the stomatogastric ganglion (STG) of the crab, Cancer borealis, as temperature was altered from 11°C to 21°C. Under control conditions, rhythm frequency increased monotonically with temperature, while the phases of the pyloric dilator (PD), lateral pyloric (LP), and pyloric (PY) neurons remained constant. Blocking Ih with cesium (Cs+) phase advanced PD offset, LP onset, and LP offset at 11°C, and the latter two further advanced as temperature increased. In Cs+ the frequency increase with temperature diminished and the Q10 of the frequency dropped from ~1.75 to ~1.35. Unexpectedly in Cs+, the frequency dynamics became non-monotonic during temperature transitions; frequency initially dropped as temperature increased, then rose once temperature stabilized, creating a characteristic ‘jag’. Interestingly, these jags persisted during temperature transitions in Cs+ when the pacemaker was isolated by picrotoxin, although the temperature-induced change in frequency recovered to control levels. Overall, these data suggest that Ih plays an important role in maintaining smooth transitory responses and persistent frequency increases by different mechanisms in the pyloric circuitry during temperature fluctuations.
Phosphodiesterase 1A physically interacts with YTHDF2 and reinforces the progression of non-small cell lung cancer
Authors: Zhang Chong , Zhang Zuoyan , Wu Yuchen , Cheng Jing , Luo Kaizhi , Li Zhidi , Zhang Manman , Wang Jian , Li Yangling
Published: October 2024

Non-small cell lung cancer (NSCLC) is the most common subtype of lung cancer, and the prognosis is poor due to distant metastasis and drug resistance. Thus, there is an urgent need to discover novel therapeutic targets and strategies to overcome cisplatin resistance and metastasis. A series of in vitro and in vivo phenotype experiments were performed to investigate the role of PDE1A in NSCLC. The RIP assay, mRNA stability assay and LC- MS/MS were performed to investigate the molecular mechanisms of PDE1A in NSCLC progression. We demonstrated that phosphodiesterase 1A (PDE1A) promoted metastasis and EMT progression of NSCLC. In addition, NSCLC cells overexpressing PDE1A promoted angiogenesis by regulating exosome release. IL-6/JAK/STAT3 signaling pathway was highly enriched in PDE1A- coexpresssed genes, and PDE1A promoted NSCLC metastasis by activating the STAT3 pathway. GO enrichment analysis of PDE1A-interacting genes showed that PDE1A might interact with YTHDF2 and participate in m6A- containing RNA binding. The binding between PDE1A and YTHDF2 was verified, and PDE1A regulated the STAT3 pathway by interacting with YTHDF2. The mechanism of YTHDF2/PDE1A complex in regulating STAT3 pathway was predicted by overlapping YTHDF2-interacting-RNAs, and genes coexpressed with YTHDF2 and STAT3. The interactions between YTHDF2 and target mRNAs were predicted, and there were three predicted targets of YTHDF2 with high scores: NRF2, SOCS2, and MET. Indeed, PDE1A interacted with YTHDF2, destabilized SOCS2, and activated STAT3 pathway. Moreover, PDE1A suppression sensitized anti-NSCLC activity of cisplatin via regulating NRF2 and MET. This work not only uncovers a novel PDE1A/YTHDF2/STAT3 pathway in NSCLC progression but also provides therapeutic strategies for treating NSCLC patients with metastasis or cisplatin- resistance.
PRR adjuvants restrain high stability peptides presentation on APCs
Authors: Li Bin , Zhang Jin , He Taojun , Yuan Hanmei , Wu Hui , Wang Peng , Wu Chao
Published: October 2024

Adjuvants can affect APCs function and boost adaptive immune responses post-vaccination. However, whether they modulate the specificity of immune responses, particularly immunodominant epitope responses, and the mechanisms of regulating antigen processing and presentation remain poorly defined. Here, using overlapping synthetic peptides, we screened the dominant epitopes of Th1 responses in mice post-vaccination with different adjuvants and found that the adjuvants altered the antigen-specific CD4
+
T-cell immunodominant epitope hierarchy. MHC-II immunopeptidomes demonstrated that the peptide repertoires presented by APCs were significantly altered by the adjuvants. Unexpectedly, no novel peptide presentation was detected after adjuvant treatment, whereas peptides with high binding stability for MHC-II presented in the control group were missing after adjuvant stimulation, particularly in the MPLA- and CpG-stimulated groups. The low-stability peptide present in the adjuvant groups effectively elicited robust T-cell responses and formed immune memory. Collectively, our results suggest that adjuvants (MPLA and CpG) inhibit high-stability peptide presentation instead of revealing cryptic epitopes, which may alter the specificity of CD4
+
T-cell-dominant epitope responses. The capacity of adjuvants to modify peptide–MHC (pMHC) stability and antigen-specific T-cell immunodominant epitope responses has fundamental implications for the selection of suitable adjuvants in the vaccine design process and epitope vaccine development.
The effects of subjective family status and subjective school status on depression and suicidal ideation among adolescents: the role of anxiety and psychological resilience
Authors: Shu Zhan , Chen Shurui , Chen Hui , Chen Xianliang , Tang Huajia , Zhou Jiawei , Tian Yusheng , Wang Xiaoping , Zhou Jiansong
Published: October 2024

Background

Depression and suicidal tendencies are notably prevalent among adolescents, yet few studies have revealed the impact of social status on them. This study aimed to explore the mediating and moderating effects of anxiety and psychological resilience on family status, school status, depression, and suicidal ideation.

Methods

A total of 1,190 secondary school students aged 13 to 17 years (mean age: 13.57 ± 2.02 years) were evaluated depression, anxiety, and suicidal ideation using the PHQ-8 and GAD-7 questionnaires. Subjective family and school status were also assessed. Data analysis was conducted using Mplus, SPSS’s Process, and the RSA 3.0 plugin.

Results

Subjective family/school status, anxiety, psychological resilience, depression, and suicidal ideation were significantly correlated. Anxiety played a partial mediating role in the influence of subjective family status and subjective school on depression and suicidal ideation, and psychological resilience moderated the impact of anxiety on adolescent depression and suicidal ideation (βdepression = −0.05, p < 0.01; βsuicidal ideation = −0.06, p < 0.05).

Conclusions

Subjective family status and school status played important roles in depression and suicidal ideation in adolescents, and anxiety and psychological resilience played mediating and moderating roles.
Propuesta didáctica desde un enfoque multimodal: enseñanza del libro álbum en iv° año de educación media
Authors:   , Campos Pinto Valeria Catalina
Published: October 2024

Resumen La masificación de recursos multimodales durante la última época, ha permitido ampliar las maneras de enseñar y mediar la literatura en la escuela. El escrito presenta el desarrollo de una propuesta didáctica para la enseñanza del libro álbum en estudiantes de IV° año de educación media que responde a una problemática evidenciada en los planes y programas del currículum escolar chileno. Se revisa el desarrollo de la actividad de 10 horas pedagógicas: “Escribir y compartir un libro álbum", que forma parte de la Unidad 3: “Aprendamos a crear colectivamente” del electivo Taller de literatura en un colegio particular privado de la Región Metropolitana (RM). Esta se justifica a partir de un marco teórico que considera aspectos presentes en la pedagogía de los géneros discursos (Rose y Martin, 2012, 2018). El ciclo didáctico se articula por medio de la deconstrucción, construcción conjunta y construcción independiente del género discursivo, junto al concepto de metafunción ideacional (Halliday, 1985, 2014), que es abordado por Kress y van Leeuwen (2021). A partir del desarrollo de la secuencia didáctica, se ha demostrado que lo realizado es efectivo para mejorar la comprensión y producción del recurso multimodal libro álbum por parte de las y los estudiantes, para potenciar sus habilidades interpretativas y creativas, generando un impacto positivo en su aprendizaje y desarrollo integral.Palabras clave: libro álbum; multimodalidad; propuesta didáctica; ciclo de enseñanza.
Mental Health Needs of SOGIE Refugees in Canada: A Rapid Review
Authors: Murugan Arooran Ananthavel , Travers Robb , Louka Farid , Zaki Karen , Morton Ninomiya Melody , Coleman Todd A.
Published: October 2024

This rapid review investigated the mental health concerns of sexual orientation and gender identity expression (SOGIE) refugees in Canada. Database searches yielded 365 results across five databases, providing 12 papers for final thematic analysis once inclusion criteria were applied. Three themes emerged: (1) Stigma and discrimination negatively affected mental health and well-being; (2) SOGIE refugees faced challenges accessing services; and (3) the refugee claims process adversely affected well-being. Recommendations to improve SOGIE refugee well-being include implementing culturally safe policies in agencies, life-skills programs, and supportive spaces; promoting inclusivity, interprofessional collaboration between services, and innovative agency outreach to refugees; addressing biases in refugee claims boards and extending the refugee claim eligibility period.
Three-Dimension Grid Plate Effecting in Mandibular Fracture Fixation
Authors: Almeida Rafael Santiago de , Rodrigues Willian Caetano , Guimarães Marco Túllio Becheleni Ávila , Falci Saulo Gabriel Moreira , Kaba Shajadi Carlos Pardo , Vieira Eduardo Hochuli , Shinohara Élio Hitoshi
Published: October 2024

Introduction: The mandibular angle fractures in the region of the body represent the highest incidence of fractures in mandibles and therefore deserve special attention and care. The use of three-dimensional plates has shown good results in this type of treatment and use has increased every day.
Case Report: The authors describe two cases of mandibular fractures treated with grid miniplate. In case 1, was carried out extra-oral access for fracture reduction in angle. In case 2 was also carried out extra-oral access for fracture reduction in mandibular body. The monitoring showed the surgical success and the absence of postoperative complications. The grid miniplate has shown a great treatment option in cases of fractures of the mandibular body and angle for ease of use and lower rates of postoperative complications.
Conclusion: The use of a three-dimensional grid plate has proven to be a viable alternative for the osteosynthesis of mandibular fractures. The results have significant clinical implications and encourage its use.
The Role of Agricultural Technology in Attaining Food Security among Small Farmers in Char Area in Mymensingh District
Authors: Haider Samiul , Kabir Md.Shajahan , Nasrin Mossat Anamika , Ahmed Shekh Maruf , Jahan Khorshed
Published: October 2024

Farmers in the char areas of Bangladesh are frequently affected by food insecurity due to their low engagement in modern agriculture such as less use of modern agricultural technology. This study evaluates the socio-demographic characteristics of the sample farmers to assess their nutritional status and the factors influencing char dwellers' dietary diversity. Survey data were collected from 70 households, and focus group discussions were conducted using a semi-structured questionnaire from Nilokhiya char under the Mymensingh District of Bangladesh. An OLS regression model was used to estimate the influence of socioeconomic characteristics of sample households on the decision on factors influencing dietary diversity. The farming type of the farmers is another concern of this study where results show that 90% of farmers in the char areas rely on rice and vegetable farming, whereas more than 60% of the farmers are also involved in livestock and poultry farming at the same time. The nutritional status of char farmers shows that their diet includes a variety of foods but is deficient insufficient protein and healthy fats, which could have an impact on their overall nutrition. Cereals are a staple in the diet, as indicated by the high consumption rate (97.1%) and the substantial average intake (2.30 kg/day). Every household consumes fruits, though in smaller quantities (0.28 kg/day). Eggs are less frequently consumed (57.1%) despite a higher intake (2.64 kg/day). Dietary diversity has a strong positive correlation with supply (.913) at a 1 % significant level which means that efficient supply chains minimize delays, reduce spoilage, and ensure a continuous flow of food to markets to ensure food security. A positive correlation with total income (.373) leads to a higher total income also supports greater dietary diversity. Credit was significant at a 5% level (.267) indicating that a 1 % increase in credit leads to an increase in dietary diversity by 267%. On the contrary, Experience has a negative relationship with dietary diversity which means that experience does not influence food security. Lack of transport and communication, environmental hazards, and natural disasters are the most significant problems faced by the farmers in the char area.