bluuebunny/crossref_metadata_2025 huggingface dataset:
Heat Strain and Use of Heat Mitigation Strategies among COVID-19 Healthcare Workers Wearing Personal Protective Equipment—A Retrospective Study
Authors: Bongers Coen C. W. G. , de Korte Johannus Q. , Zwartkruis Mike , Levels Koen , Kingma Boris R. M. , Eijsvogels Thijs M. H.
Published: February 2022

The combination of an exacerbated workload and impermeable nature of the personal protective equipment (PPE) worn by COVID-19 healthcare workers increases heat strain. We aimed to compare the prevalence of heat strain symptoms before (routine care without PPE) versus during the COVID-19 pandemic (COVID-19 care with PPE), identify risk factors associated with experiencing heat strain, and evaluate the access to and use of heat mitigation strategies. Dutch healthcare workers (n = 791) working at COVID-19 wards for ≥1 week, completed an online questionnaire to assess personal characteristics, heat strain symptoms before and during the COVID-19 pandemic, and the access to and use of heat mitigation strategies. Healthcare workers experienced ~25× more often heat strain symptoms during medical duties with PPE (93% of healthcare workers) compared to without PPE (30% of healthcare workers; OR = 25.57 (95% CI = 18.17–35.98)). Female healthcare workers and those with an age <40 years were most affected by heat strain, whereas exposure time and sports activity level were not significantly associated with heat strain prevalence. Cold drinks and ice slurry ingestion were the most frequently used heat mitigation strategies and were available in 63.5% and 30.1% of participants, respectively. Our findings indicate that heat strain is a major challenge for COVID-19 healthcare workers, and heat mitigations strategies are often used to counteract heat strain.
Global Perspective of Legionella Infection in Community-Acquired Pneumonia: A Systematic Review and Meta-Analysis of Observational Studies
Authors: Graham Frances F. , Finn Norah , White Paul , Hales Simon , Baker Michael G.
Published: February 2022

Legionnaires’ disease (LD) (Legionella) is a common cause of community-acquired pneumonia (CAP) in those requiring hospitalization. Geographical variation in the importance of Legionella species as an aetiologic agent of CAP is poorly understood. We performed a systematic review and meta-analysis of population-based observational studies that reported the proportion of Legionella infection in patients with CAP (1 January 1990 to 31 May 2020). Using five electronic databases, articles were identified, appraised and reported in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. The quality of the included studies was assessed using the Newcastle–Ottawa Scale. Univariate and multivariate meta-regression analyses were conducted using study design, WHO region, study quality and healthcare setting as the explanatory variables. We reviewed 2778 studies, of which 219 were included in the meta-analysis. The mean incidence of CAP was 46.7/100,000 population (95% CI: 46.6–46.8). The mean proportion of Legionella as the causative agent for CAP was 4.6% (95% CI: 4.4 to 4.7). Consequently, the mean Legionella incidence rate was 2.8/100,000 population (95% CI: 2.7–2.9). There was significant heterogeneity across all studies I2 = 99.27% (p < 0.0001). After outliers were removed, there was a decrease in the heterogeneity (I2 = 43.53%). Legionella contribution to CAP has a global distribution. Although the rates appear highest in high income countries in temperate regions, there are insufficient studies from low- and middle-income countries to draw conclusions about the rates in these regions. Nevertheless, this study provides an estimate of the mean incidence of Legionella infection in CAP, which could be used to estimate the regional and global burden of LD to support efforts to reduce the impact of this infection as well as to fill important knowledge gaps.
Estonian Dental Students’ Oral Health-Related Knowledge, Attitudes and Behaviours (KAB): National Survey-Based Study
Authors: Riad Abanoub , Põld Ave , Olak Jana , Howaldt Hans-Peter , Klugar Miloslav , Krsek Martin , Attia Sameh
Published: February 2022

The strategic plan for dentistry and oral health in Estonia of 2030 focuses on oral health promotion and disease prevention through undergraduate dental curricula and fostering public health-oriented research among students. The present study was designed as a descriptive cross-sectional study to evaluate oral health-related knowledge, attitudes, and behaviours (KAB) of dental students in Estonia. The study was carried out in the spring semester of 2020, and it used a modified version of the Hiroshima University Dental Behavioural Inventory (HU-DBI). A total of 129 students responded to the survey, constituting a response rate of 93.5% due to the total population sampling (census) technique used in this study and the small target population size. Out of the 124 students included in the final analysis, 79% were females, 62.1% were clinical students, 11.3% reported smoking tobacco at least once a week, and 86.3% reported problematic internet use. The present study found that mean HU-DBI score of Estonian dental students was 8.09 ± 1.22 which is so far the highest recorded HU-DBI score in Europe. There was no significant difference between female vs. male or preclinical vs. clinical students in terms of HU-DBI score. While clinical students reported less faulty oral hygiene practices, such as hard toothbrush use and aggressive toothbrushing, preclinical students reported a slightly higher mean HU-DBI score. Smoking behaviour was more common among male and clinical students, and it was also associated with alcohol drinking and worry about teeth colour and halitosis.
Precarious Employment and Chronic Stress: Do Social Support Networks Matter?
Authors: Belvis Francesc , Bolíbar Mireia , Benach Joan , Julià Mireia
Published: February 2022

Precarious employment has been identified as a potentially damaging stressor. Conversely, social support networks have a well-known protective effect on health and well-being. The ways in which precariousness and social support may interact have scarcely been studied with respect to either perceived stress or objective stress biomarkers. This research aims to fill this gap by means of a cross-sectional study based on a non-probability quota sample of 250 workers aged 25–60 in Barcelona, Spain. Fieldwork was carried out between May 2019 and January 2020. Employment precariousness, perceived social support and stress levels were measured by means of scales, while individual steroid profiles capturing the chronic stress suffered over a period of a month were obtained from hair samples using a liquid chromatography-tandem mass spectrometry methodology. As for perceived stress, analysis indicates that a reverse buffering effect exists (interaction B = 0.22, p = 0.014). Steroid biomarkers are unrelated to social support, while association with precariousness is weak and only reaches significance at p < 0.05 in the case of women and 20ß dihydrocortisone metabolites. These results suggest that social support can have negative effects on the relationship between perceived health and an emerging stressful condition like precariousness, while its association with physiological measures of stress remains uncertain.
Physiologic Insulin Resensitization as a Treatment Modality for Insulin Resistance Pathophysiology
Authors: Greenway Frank , Loveridge Brian , Grimes Richard M. , Tucker Tori R. , Alexander Michael , Hepford Scott A. , Fontenot Justin , Nobles-James Candi , Wilson Carol , Starr Adam M. , Abdelsaid Mohammed , Lewis Stanley T. , Lakey Jonathan R. T.
Published: February 2022

Prevalence of type 2 diabetes increased from 2.5% of the US population in 1990 to 10.5% in 2018. This creates a major public health problem, due to increases in long-term complications of diabetes, including neuropathy, retinopathy, nephropathy, skin ulcers, amputations, and atherosclerotic cardiovascular disease. In this review, we evaluated the scientific basis that supports the use of physiologic insulin resensitization. Insulin resistance is the primary cause of type 2 diabetes. Insulin resistance leads to increasing insulin secretion, leading to beta-cell exhaustion or burnout. This triggers a cascade leading to islet cell destruction and the long-term complications of type 2 diabetes. Concurrent with insulin resistance, the regular bursts of insulin from the pancreas become irregular. This has been treated by the precise administration of insulin more physiologically. There is consistent evidence that this treatment modality can reverse the diabetes-associated complications of neuropathy, diabetic ulcers, nephropathy, and retinopathy, and that it lowers HbA1c. In conclusion, physiologic insulin resensitization has a persuasive scientific basis, significant treatment potential, and likely cost benefits.
Antimicrobial Materials with Medical Applications
Authors: Banti Christina N. , Hadjikakou Sotiris K.
Published: February 2022

This Special Issue of the International Journal of Molecular Sciences, entitled “Antimicrobial Materials with Medical Applications”, covers a selection of recent research and review articles in the field of antimicrobial materials, as well as their medical applications [...]
Synergistic Effect of Co-Delivering Ciprofloxacin and Tetracycline Hydrochloride for Promoted Wound Healing by Utilizing Coaxial PCL/Gelatin Nanofiber Membrane
Authors: Lin Mengxia , Liu Yuan , Gao Junwei , Wang Donghui , Xia Dan , Liang Chunyong , Li Ning , Xu Ruodan
Published: February 2022

Combining multiple drugs or biologically active substances for wound healing could not only resist the formation of multidrug resistant pathogens, but also achieve better therapeutic effects. Herein, the hydrophobic fluoroquinolone antibiotic ciprofloxacin (CIP) and the hydrophilic broad-spectrum antibiotic tetracycline hydrochloride (TH) were introduced into the coaxial polycaprolactone/gelatin (PCL/GEL) nanofiber mat with CIP loaded into the PCL (core layer) and TH loaded into the GEL (shell layer), developing antibacterial wound dressing with the co-delivering of the two antibiotics (PCL-CIP/GEL-TH). The nanostructure, physical properties, drug release, antibacterial property, and in vitro cytotoxicity were investigated accordingly. The results revealed that the CIP shows a long-lasting release of five days, reaching the releasing rate of 80.71%, while the cumulative drug release of TH reached 83.51% with a rapid release behavior of 12 h. The in vitro antibacterial activity demonstrated that the coaxial nanofiber mesh possesses strong antibacterial activity against E. coli and S. aureus. In addition, the coaxial mats showed superior biocompatibility toward human skin fibroblast cells (hSFCs). This study indicates that the developed PCL-CIP/GEL-TH nanofiber membranes hold enormous potential as wound dressing materials.
Overexpression of MdZAT5, an C2H2-Type Zinc Finger Protein, Regulates Anthocyanin Accumulation and Salt Stress Response in Apple Calli and Arabidopsis
Authors: Wang Da-Ru , Yang Kuo , Wang Xun , Lin Xiao-Lu , Rui Lin , Liu Hao-Feng , Liu Dan-Dan , You Chun-Xiang
Published: February 2022

Zinc finger proteins are widely involved and play an important role in plant growth and abiotic stress. In this research, MdZAT5, a gene encoding C2H2-type zinc finger protein, was cloned and investigated. The MdZAT5 was highly expressed in flower tissues by qRT-PCR analyses and GUS staining. Promoter analysis showed that MdZAT5 contained multiple response elements, and the expression levels of MdZAT5 were induced by various abiotic stress treatments. Overexpression of MdZAT5 in apple calli positively regulated anthocyanin accumulation by activating the expressions of anthocyanin biosynthesis-related genes. Overexpression of MdZAT5 in Arabidopsis also enhanced the accumulation of anthocyanin. In addition, MdZAT5 increased the sensitivity to salt stress in apple calli. Ectopic expression of MdZAT5 in Arabidopsis reduced the expression of salt-stress-related genes (AtNHX1 and AtABI1) and improved the sensitivity to salt stress. In conclusion, these results suggest that MdZAT5 plays a positive regulatory role in anthocyanin accumulation and negatively regulates salt resistance.
Identification of Corosolic and Oleanolic Acids as Molecules Antagonizing the Human RORγT Nuclear Receptor Using the Calculated Fingerprints of the Molecular Similarity
Authors: Pastwińska Joanna , Karaś Kaja , Sałkowska Anna , Karwaciak Iwona , Chałaśkiewicz Katarzyna , Wojtczak Błażej A. , Bachorz Rafał A. , Ratajewski Marcin
Published: February 2022

RORγT is a protein product of the RORC gene belonging to the nuclear receptor subfamily of retinoic-acid-receptor-related orphan receptors (RORs). RORγT is preferentially expressed in Th17 lymphocytes and drives their differentiation from naive CD4+ cells and is involved in the regulation of the expression of numerous Th17-specific cytokines, such as IL-17. Because Th17 cells are implicated in the pathology of autoimmune diseases (e.g., psoriasis, inflammatory bowel disease, multiple sclerosis), RORγT, whose activity is regulated by ligands, has been recognized as a drug target in potential therapies against these diseases. The identification of such ligands is time-consuming and usually requires the screening of chemical libraries. Herein, using a Tanimoto similarity search, we found corosolic acid and other pentacyclic tritepenes in the library we previously screened as compounds highly similar to the RORγT inverse agonist ursolic acid. Furthermore, using gene reporter assays and Th17 lymphocytes, we distinguished compounds that exert stronger biological effects (ursolic, corosolic, and oleanolic acid) from those that are ineffective (asiatic and maslinic acids), providing evidence that such combinatorial methodology (in silico and experimental) might help wet screenings to achieve more accurate results, eliminating false negatives.
A Real-Time, Plate-Based BRET Assay for Detection of cGMP in Primary Cells
Authors: Valkovic Adam L. , Kocan Martina , Hoare Brad , Marshall Sarah , Scott Daniel J. , Bathgate Ross A. D.
Published: February 2022

Cyclic guanosine monophosphate (cGMP) is a second messenger involved in the regulation of numerous physiological processes. The modulation of cGMP is important in many diseases, but reliably assaying cGMP in live cells in a plate-based format with temporal resolution is challenging. The Förster/fluorescence resonance energy transfer (FRET)-based biosensor cGES-DE5 has a high temporal resolution and high selectivity for cGMP over cAMP, so we converted it to use bioluminescence resonance energy transfer (BRET), which is more compatible with plate-based assays. This BRET variant, called CYGYEL (cyclic GMP sensor using YFP-PDE5-Rluc8), was cloned into a lentiviral vector for use across different mammalian cell types. CYGYEL was characterised in HEK293T cells using the nitric oxide donor diethylamine NONOate (DEA), where it was shown to be dynamic, reversible, and able to detect cGMP with or without the use of phosphodiesterase inhibitors. In human primary vascular endothelial and smooth muscle cells, CYGYEL successfully detected cGMP mediated through either soluble or particulate guanylate cyclase using DEA or C-type natriuretic peptide, respectively. Notably, CYGYEL detected differences in kinetics and strength of signal both between ligands and between cell types. CYGYEL remained selective for cGMP over cAMP, but this selectivity was reduced compared to cGES-DE5. CYGYEL streamlines the process of cGMP detection in plate-based assays and can be used to detect cGMP activity across a range of cell types.
Fusarium graminearum Infection Strategy in Wheat Involves a Highly Conserved Genetic Program That Controls the Expression of a Core Effectome
Authors: Rocher Florian , Alouane Tarek , Philippe Géraldine , Martin Marie-Laure , Label Philippe , Langin Thierry , Bonhomme Ludovic
Published: February 2022

Fusarium graminearum, the main causal agent of Fusarium Head Blight (FHB), is one of the most damaging pathogens in wheat. Because of the complex organization of wheat resistance to FHB, this pathosystem represents a relevant model to elucidate the molecular mechanisms underlying plant susceptibility and to identify their main drivers, the pathogen’s effectors. Although the F. graminearum catalog of effectors has been well characterized at the genome scale, in planta studies are needed to confirm their effective accumulation in host tissues and to identify their role during the infection process. Taking advantage of the genetic variability from both species, a RNAseq-based profiling of gene expression was performed during an infection time course using an aggressive F. graminearum strain facing five wheat cultivars of contrasting susceptibility as well as using three strains of contrasting aggressiveness infecting a single susceptible host. Genes coding for secreted proteins and exhibiting significant expression changes along infection progress were selected to identify the effector gene candidates. During its interaction with the five wheat cultivars, 476 effector genes were expressed by the aggressive strain, among which 91% were found in all the infected hosts. Considering three different strains infecting a single susceptible host, 761 effector genes were identified, among which 90% were systematically expressed in the three strains. We revealed a robust F. graminearum core effectome of 357 genes expressed in all the hosts and by all the strains that exhibited conserved expression patterns over time. Several wheat compartments were predicted to be targeted by these putative effectors including apoplast, nucleus, chloroplast and mitochondria. Taken together, our results shed light on a highly conserved parasite strategy. They led to the identification of reliable key fungal genes putatively involved in wheat susceptibility to F. graminearum, and provided valuable information about their putative targets.
Screening of Differentially Expressed Genes and Localization Analysis of Female Gametophyte at the Free Nuclear Mitosis Stage in Pinus tabuliformis Carr.
Authors: Gong Zaixin , Hu Hailin , Xu Li , Zhao Yuanyuan , Zheng Caixia
Published: February 2022

Female sterility is a common phenomenon in the plant world, and systematic research has not been carried out in gymnosperms. In this study, the ovules of No. 28 sterile line and No. 15 fertile line Pinus tabuliformis were used as materials, and a total of 18 cDNA libraries were sequenced by the HiSeqTM 4000 platform to analyze the differentially expressed genes (DEGs) and simple sequence repeats (SSRs) between the two lines. In addition, this study further analyzed the DEGs involved in the signal transduction of plant hormones, revealing that the signal pathways related to auxin, cytokinin, and gibberellin were blocked in the sterile ovule. Additionally, real-time fluorescent quantitative PCR verified that the expression trend of DEGs related to plant hormones was consistent with the results of high-throughput sequencing. Frozen sections and fluorescence in situ hybridization (FISH) were used to study the temporal and spatial expression patterns of PtRab in the ovules of P. tabuliformis. It was found that PtRab was significantly expressed in female gametophytes and rarely expressed in the surrounding diploid tissues. This study further explained the molecular regulation mechanism of female sterility in P. tabuliformis, preliminarily mining the key factors of ovule abortion in gymnosperms at the transcriptional level.
Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients
Authors: Sot Jesús , García-Arribas Aritz , Abad Beatriz , Arranz Sara , Portune Kevin , Andrade Fernando , Martín-Nieto Alicia , Velasco Olaia , Arana Eunate , Tueros Itziar , Ferreri Carla , Gaztambide Sonia , Goñi Félix , Castaño Luis , Alonso Alicia
Published: February 2022

This work intends to describe the physical properties of red blood cell (RBC) membranes in obese adults. The hypothesis driving this research is that obesity, in addition to increasing the amount of body fat, will also modify the lipid composition of membranes in cells other than adipocytes. Forty-nine control volunteers (16 male, 33 female, BMI 21.8 ± 5.6 and 21.5 ± 4.2 kg/m2, respectively) and 52 obese subjects (16 male and 36 female, BMI 38.2± 11.0 and 40.7 ± 8.7 kg/m2, respectively) were examined. The two physical techniques applied were atomic force microscopy (AFM) in the force spectroscopy mode, which allows the micromechanical measurement of penetration forces, and fluorescence anisotropy of trimethylammonium diphenylhexatriene (TMA-DPH), which provides information on lipid order at the membrane polar–nonpolar interface. These techniques, in combination with lipidomic studies, revealed a decreased rigidity in the interfacial region of the RBC membranes of obese as compared to control patients, related to parallel changes in lipid composition. Lipidomic data show an increase in the cholesterol/phospholipid mole ratio and a decrease in sphingomyelin contents in obese membranes. ω-3 fatty acids (e.g., docosahexaenoic acid) appear to be less prevalent in obese patient RBCs, and this is the case for both the global fatty acid distribution and for the individual major lipids in the membrane phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS). Moreover, some ω-6 fatty acids (e.g., arachidonic acid) are increased in obese patient RBCs. The switch from ω-3 to ω-6 lipids in obese subjects could be a major factor explaining the higher interfacial fluidity in obese patient RBC membranes.
Recent Advance in Biological Responsive Nanomaterials for Biosensing and Molecular Imaging Application
Authors: Jiang Zhenqi , Han Xiao , Zhao Chen , Wang Shanshan , Tang Xiaoying
Published: February 2022

In recent decades, as a subclass of biomaterials, biologically sensitive nanoparticles have attracted increased scientific interest. Many of the demands for physiologically responsive nanomaterials in applications involving the human body cannot be met by conventional technologies. Due to the field’s importance, considerable effort has been expended, and biologically responsive nanomaterials have achieved remarkable success thus far. This review summarizes the recent advancements in biologically responsive nanomaterials and their applications in biosensing and molecular imaging. The nanomaterials change their structure or increase the chemical reaction ratio in response to specific bio-relevant stimuli (such as pH, redox potentials, enzyme kinds, and concentrations) in order to improve the signal for biologically responsive diagnosis. We use various case studies to illustrate the existing issues and provide a clear sense of direction in this area. Furthermore, the limitations and prospects of these nanomaterials for diagnosis are also discussed.
Inherited Cancer Genetic Epidemiology to Improve Precision Medicine
Authors: Møller Pål , Evans Dafydd Gareth
Published: February 2022

Epidemiology is the study of the distribution and determinants of health-related states or events in specified populations, and the application of this study to the control of health problems [...]
Characteristics and Outcomes of Bloodstream Infections in a Tertiary-Care Pediatric Hematology–Oncology Unit: A 10-Year Study
Authors: Mattei Davide , Baretta Valentina , Mazzariol Annarita , Maccacaro Laura , Balter Rita , Zaccaron Ada , Bonetti Elisa , Chinello Matteo , Vitale Virginia , Caddeo Giulia , Esposto Maria , Pezzella Vincenza , Gibellini Davide , Tridello Gloria , Cesaro Simone
Published: February 2022

Bloodstream infections (BSIs) after chemotherapy or hematopoietic stem cell transplantation (HSCT) are a leading cause of morbidity and mortality. Data on 154 BSIs that occurred in 111 onco-hematological patients (57 hematological malignancies, 28 solid tumors, and 26 non-malignant hematological diseases) were retrospectively collected and analyzed. Monomicrobial Gram-positive (GP), Gram-negative (GN), and fungal BSIs accounted for 50% (77/154), 38.3% (59/144), and 3.2% (5/154) of all episodes. Polymicrobial infections were 7.8% (12/154), while mixed bacterial–fungal infections were 0.6% (1/154). The most frequent GN isolates were Escherichia coli (46.9%), followed by Pseudomonas aeruginosa (21.9%), Klebsiella species (18.8%), and Enterobacter species (6.3%). Overall, 18.8% (12/64) of GN organisms were multidrug-resistant (seven Escherichia coli, three Klebsiella pneumoniae, and two Enterobacter cloacae), whereas GP resistance to glycopeptides was observed in 1% (1/97). Initial empirical antibiotic therapy was deemed inappropriate in 12.3% of BSIs (19/154). The 30-day mortality was 7.1% (11/154), while the bacteremia-attributable mortality was 3.9% (6/154). In multivariate analysis, septic shock was significantly associated with 30-day mortality (p = 0.0001). Attentive analysis of epidemiology and continuous microbiological surveillance are essential for the appropriate treatment of bacterial infections in pediatric onco-hematological patients.
Development and Validation of the Acute PNeumonia Early Assessment Score for Safely Discharging Low-Risk SARS-CoV-2-Infected Patients from the Emergency Department
Authors: Venturini Sergio , Pontoni Elisa , Carnelos Rossella , Arcidiacono Domenico , Da Ros Silvia , De Santi Laura , Orso Daniele , Cugini Francesco , Fossati Sara , Callegari Astrid , Mancini Walter , Tonizzo Maurizio , Grembiale Alessandro , Crapis Massimo , Colussi GianLuca
Published: February 2022

A continuous demand for assistance and an overcrowded emergency department (ED) require early and safe discharge of low-risk severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected patients. We developed (n = 128) and validated (n = 330) the acute PNeumonia early assessment (aPNea) score in a tertiary hospital and preliminarily tested the score on an external secondary hospital (n = 97). The score’s performance was compared to that of the National Early Warning Score 2 (NEWS2). The composite outcome of either death or oral intubation within 30 days from admission occurred in 101 and 28 patients in the two hospitals, respectively. The area under the receiver operating characteristic (AUROC) curve of the aPNea model was 0.86 (95% confidence interval (CI), 0.78–0.93) and 0.79 (95% CI, 0.73–0.89) for the development and validation cohorts, respectively. The aPNea score discriminated low-risk patients better than NEWS2 at a 10% outcome probability, corresponding to five cut-off points and one cut-off point, respectively. aPNea’s cut-off reduced the number of unnecessary hospitalizations without missing outcomes by 27% (95% CI, 9–41) in the validation cohort. NEWS2 was not significant. In the external cohort, aPNea’s cut-off had 93% sensitivity (95% CI, 83–102) and a 94% negative predictive value (95% CI, 87–102). In conclusion, the aPNea score appears to be appropriate for discharging low-risk SARS-CoV-2-infected patients from the ED.
Antimicrobial Meshes for Hernia Repair: Current Progress and Perspectives
Authors: Mirel Simona , Pusta Alexandra , Moldovan Mihaela , Moldovan Septimiu
Published: February 2022

Recent advances in the development of biomaterials have given rise to new options for surgery. New-generation medical devices can control chemical breakdown and resorption, prevent post-operative adhesion, and stimulate tissue regeneration. For the fabrication of medical devices, numerous biomaterials can be employed, including non-degradable biomaterials (silicone, polypropylene, expanded polytetrafluoroethylene) or biodegradable polymers, including implants and three-dimensional scaffolds for tissue engineering, which require particular physicochemical and biological properties. Based on the combination of new generation technologies and cell-based therapies, the biocompatible and bioactive properties of some of these medical products can lead to progress in the repair of injured or harmed tissue and in tissue regeneration. An important aspect in the use of these prosthetic devices is the associated infection risk, due to the medical complications and socio-economic impact. This paper provides the latest achievements in the field of antimicrobial surgical meshes for hernia repair and discusses the perspectives in the development of these innovative biomaterials.
Kidney Function According to Different Equations in Patients Admitted to a Cardiology Unit and Impact on Outcome
Authors: Malavasi Vincenzo Livio , Valenti Anna Chiara , Ruggerini Sara , Manicardi Marcella , Orlandi Carlotta , Sgreccia Daria , Vitolo Marco , Proietti Marco , Lip Gregory Y. H. , Boriani Giuseppe
Published: February 2022

Background: This paper aims to evaluate the concordance between the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) formula and alternative equations and to assess their predictive power for all-cause mortality in unselected patients discharged alive from a cardiology ward. Methods: We retrospectively included patients admitted to our Cardiology Division independently of their diagnosis. The total population was classified according to Kidney Disease: Improving Global Outcomes (KDIGO) categories, as follows: G1 (estimated glomerular filtration rate (eGFR) ≥90 mL/min/1.73 m2); G2 (eGFR 89–60 mL/min/1.73 m2); G3a (eGFR 59–45 mL/min/1.73 m2); G3b (eGFR 44–30 mL/min/1.73 m2); G4 (eGFR 29–15 mL/min/1.73 m2); G5 (eGFR <15 mL/min/1.73 m2). Cockcroft-Gault (CG), CG adjusted for body surface area (CG-BSA), Modification of Diet in Renal Disease (MDRD), Berlin Initiative Study (BIS-1), and Full Age Spectrum (FAS) equations were also assessed. Results: A total of 806 patients were included. Good agreement was found between the CKD-EPI formula and CG-BSA, MDRD, BIS-1, and FAS equations. In subjects younger than 65 years or aged ≥85 years, CKD-EPI and MDRD showed the highest agreement (Cohen’s kappa (K) 0.881 and 0.588, respectively) while CG showed the lowest. After a median follow-up of 407 days, overall mortality was 8.2%. The risk of death was higher in lower eGFR classes (G3b HR4.35; 95%CI 1.05–17.80; G4 HR7.13; 95%CI 1.63–31.23; G5 HR25.91; 95%CI 6.63–101.21). The discriminant capability of death prediction tested with ROC curves showed the best results for BIS-1 and FAS equations. Conclusion: In our cohort, the concordance between CKD-EPI and other equations decreased with age, with the MDRD formula showing the best agreement in both younger and older patients. Overall, mortality rates increased with the renal function decreasing. In patients aged ≥75 years, the best discriminant capability for death prediction was found for BIS-1 and FAS equations.
A Systematic Literature Review of the Relationship between Serum Ferritin and Outcomes in Myelodysplastic Syndromes
Authors: Oliva Esther Natalie , Huey Krystal , Deshpande Sohan , Turner Monica , Chitnis Madhura , Schiller Emma , Tang Derek , Yucel Aylin , Hughes Christina , Shah Farrukh
Published: February 2022

Anemia is the most common form of cytopenia in patients with myelodysplastic syndromes (MDS), who require chronic red blood cell transfusions and may present high serum ferritin (SF) levels as a result of iron overload. To better understand the potential effects of high SF levels, we conducted a systematic literature review (SLR) to identify evidence on the relationship between SF levels and clinical, economic, or humanistic outcomes in adult patients with MDS. Of 267 references identified, 21 were included. No studies assessing SF levels and their relationship with humanistic or economic outcomes were identified. Increased SF levels were an indicator of worse overall survival and other worsened outcomes; however, the association was not consistently significant. SF levels were a significant prognostic factor for relapse incidence of MDS and showed a significant positive correlation with number of blood units transfused but were not associated with progression to acute myeloid leukemia or the time to transformation. Higher SF levels were also an indicator of a lower likelihood of leukemia-free survival, relapse-free survival, and event-free survival. The SLR suggests that SF levels are associated with clinical outcomes in MDS, with higher levels correlated with number of blood units transfused, frequently indicating worse outcomes.
The Influence of Angle Alpha, Angle Kappa, and Optical Aberrations on Visual Outcomes after the Implantation of a High-Addition Trifocal IOL
Authors: Cervantes-Coste Guadalupe , Tapia André , Corredor-Ortega Claudia , Osorio Mariana , Valdez Rafael , Massaro Martha , Velasco-Barona Cecilio , Gonzalez-Salinas Roberto
Published: February 2022

The aim of our investigation was to examine the possible correlations between optical aberrations, angle kappa, angle alpha, and visual outcomes following cataract surgery. In total, 56 eyes of 28 patients were implanted with the Liberty 677MY trifocal intraocular lens (IOL). Pre- and postoperative higher-order aberrations, coma, astigmatism, angle alpha, and angle kappa were registered, along with uncorrected and corrected visual acuities at multiple distances. Visual acuity and contrast sensitivity defocus curves were plotted, and the areas under the curve were calculated 1 and 3 months postoperatively. Excellent visual outcomes were found at all distances. Patients reported low levels of dysphotopsia, and 96.4% of patients achieved complete spectacle independence. While angle kappa significantly decreased during cataract surgery (p = 0.0007), angle alpha remained unchanged (p = 0.5158). Angle alpha correlated with postoperative HOAs and had a negative impact on near vision (p = 0.0543). Preoperative corneal HOA and coma had a strong adverse effect on future intermediate and near vision. Residual astigmatism significantly affected postoperative intermediate vision (p = 0.0091). Our results suggest that angle kappa is not an optimal predictive factor for future visual outcomes, while angle alpha and the preoperative screening of optical aberrations might help patient selection prior to multifocal IOL implantation.
Fatigue Analysis of the Oil Offloading Lines in FPSO System under Wave and Current Loads
Authors: Zhang Xu , Ni Wenchi , Sun Liping
Published: February 2022

In this paper, fatigue analysis of oil offloading lines (OOLs) in the floating production storage and offloading (FPSO) catenary anchor leg mooring (CALM) buoy offloading system under wave and current loads in the West Africa Sea area is carried out by the numerical simulation method. The hydrodynamic coupling response is calculated, and fatigue damage is analyzed. Firstly, the numerical model is verified by comparison with the experimental results. Then, according to the environmental statistics in West Africa, the influence of various parameters on the fatigue damage of OOLs is analyzed, including tension characteristics, wave parameters, and structural parameters. Additionally, the effect of current load is studied. Results show that accumulated fatigue damage mainly occurs near the CALM buoy and is mainly caused by the 0° wind wave. Appropriately reducing the cover length of buoyancy material and increasing the wall thickness can reduce fatigue damage. Moreover, the effect of the shuttle tanker can increase the fatigue damage of the OOL near the CALM buoy by about 1.5 times, and the effect of vortex-induced vibration can increase the fatigue damage of the OOL in the middle part by up to 5–10 times.
Review of the Influence of Oceanographic and Geometric Parameters on Oscillating Water Columns
Authors: Portillo Juan Nerea , Negro Valdecantos Vicente , Esteban M. Dolores , López Gutiérrez José Santos
Published: February 2022

Wave energy is one of the most powerful sources of energy on our planet, but its exploitation is difficult. Much current research on renewable energy is focused on how to harness ocean energy. However, wave energy converter (WEC) technology is still immature and how to reach high levels of efficiency is still unknown. In coming years, this field is likely to reach a high level of development, so it is important to continue research on the improvement of the performance of these devices. One of the most important wave energy converters is the oscillating water column (OWC). The main difficulty of OWCs is that they have to provide good rates of hydrodynamic efficiency for many different types of sea states (different periods, heights, wavelengths, etc.). The other big concern is the optimization of the geometric parameters of the device. This research paper is focused on these two big concerns: how oceanographic parameters affect the hydrodynamic behavior of an OWC and its geometric optimization. Different studies about how wave and geometric characteristics affect the performance of an OWC are reviewed and relationships between these and the hydrodynamic performance of an OWC are finally outlined and summed up.
Robust Composite Dynamic Event-Triggered Control for Multiple USVs with DLLOS Guidance
Authors: Zhang Guoqing , Liu Shang , Li Bo , Zhang Xianku
Published: February 2022

In this paper, a robust composite dynamic event-triggered formation control scheme is proposed for multiple underactuated surface vehicles (USVs) from two aspects, i.e., guidance and control. In the guidance module, a novel dual-layer line-of-sight (DLLOS) guidance principle is incorporated into the leader–follower framework to generate the reference path. To overcome the problem of unavailable leader velocity information, an adaptive speed controller is designed to adjust the navigational speed of followers. As for the control part, by utilizing the dynamic event-triggered method, the operational frequency of actuators can be reduced in a flexible manner. That can effectively avoid the excessive wear and chattering phenomenon of actuators. Furthermore, by the fusing of the radial basis function neural networks (RBF NNs) and the robust neural damping technique, the model uncertainty, environmental disturbances and some unknown parameters can be remodeled, and only two gain-related adaptive laws need to be updated online. The serial–parallel estimation model (SPEM) is established to predict the velocity variables, and the approximation performance of NNs can be enhanced by virtue of the derived prediction error. Through the Lyapunov stable theorem, all control signals in the closed-loop system are guaranteed semi-globally uniformly ultimately bounded (SGUUB) stability. Finally, digital simulations are illustrated to verify the effectiveness and superiority of the proposed algorithm.
A Numerical Study of a Submerged Water Jet Impinging on a Stationary Wall
Authors: Hu Bo , Wang Hui , Liu Jinhua , Zhu Yong , Wang Chuan , Ge Jie , Zhang Yingchong
Published: February 2022

The impinging jet is a classical flow model with relatively simple geometric boundary conditions, and it is widely used in marine engineering. In recent years, scholars have conducted more and more fundamental studies on impact jets, but most of the classical turbulence models are used in numerical simulations, and the accuracy of their calculation results is still a problem in regions with large changes in velocity gradients such as the impact zone. In order to study the complex flow characteristics of the water flow under the condition of a submerged jet impacting a stationary wall, the Wray–Agarwal turbulence model was chosen for the Computational Fluid Dynamics (CFD) numerical simulation study of the impacting jet. Continuous jets with different Reynolds numbers and different impact heights H/D were used to impact the stationary wall, and the results show that the jet flow structure depends on the impact height and is relatively independent of the Reynolds number. With the increase in the impact height, the diffusion of the jet reaching the impact area gradually increases, and its velocity gradually decreases. As the impact height increases, the maximum pressure coefficient decreases and the rate of decrease increases gradually, and the dimensionless pressure distribution is almost constant. In this paper, the flow field structure and pressure characteristics of a continuous submerged jet impacting a stationary wall are explored in depth, which is of great guidance to engineering practice.
Clinical Utility and Validation of the Krakow DCM Risk Score—A Prognostic Model Dedicated to Dilated Cardiomyopathy
Authors: Dziewięcka Ewa , Winiarczyk Mateusz , Wiśniowska-Śmiałek Sylwia , Karabinowska-Małocha Aleksandra , Gliniak Matylda , Robak Jan , Kaciczak Monika , Leszek Przemysław , Celińska-Spodar Małgorzata , Dziewięcki Marcin , Rubiś Paweł
Published: February 2022

Background: One of the most common causes of heart failure is dilated cardiomyopathy (DCM). In DCM, the mortality risk is high and reaches approximately 20% in 5 years. A patient’s prognosis should be established for appropriate HF management. However, so far, no validated tools have been available for the DCM population. Methods: The study population consisted of 735 DCM patients: 406 from the derivation cohort (previously described) and 329 from the validation cohort (from 2009 to 2020, with outcome data after a mean of 42 months). For each DCM patient, the individual mortality risk was calculated based on the Krakow DCM Risk Score. Results: During follow-up, 49 (15%) patients of the validation cohort died. They had shown significantly higher calculated 1-to-5-year mortality risks. The Krakow DCM Risk Score yielded good discrimination in terms of overall mortality risk, with an AUC of 0.704–0.765. Based on a 2-year mortality risk, patients were divided into non-high (≤6%) and high (>6%) mortality risk groups. The observed mortality rates were 8.3% (n = 44) vs. 42.6% (n = 75), respectively (HR 3.37; 95%CI 1.88–6.05; p < 0.0001). Conclusions: The Krakow DCM Risk Score was found to have good predictive accuracy. The 2-year mortality risk > 6% has good discrimination for the identification of high-risk patients and can be applied in everyday practice.
Long-Term Outcome of HBV-Infected Patients with Clinically Significant Portal Hypertension Achieving Viral Suppression
Authors: Jachs Mathias , Hartl Lukas , Bauer David , Simbrunner Benedikt , Stättermayer Albert , Strassl Robert , Trauner Michael , Mandorfer Mattias , Reiberger Thomas
Published: February 2022

Background: Nucleos(t)ide analog (NA) treatment for hepatitis B virus (HBV) infection may improve clinically significant portal hypertension (CSPH). Data on hepatic venous pressure gradient (HVPG) and non-invasive tests (NITs) for risk re-stratification in virally suppressed HBV-infected patients with pre-treatment CSPH are limited. Methods: We retrospectively included patients with long-term (>12 months) suppression of HBV replication and pre-treatment CSPH (i.e., varices, collaterals on cross-sectional imaging, or ascites). Patients were monitored by on-treatment liver stiffness measurement (LSM) and HVPG assessment. The primary outcome was (further) hepatic decompensation (including liver-related mortality). Results: Forty-two patients (n = 12 (28.6%) with previous decompensation, HBeAg-negative: n = 36 (85.7%)) were included and followed for 2.1 (0.6; 5.3) years. The median HVPG (available in n = 17) was 15 (10; 22) mmHg and the median LSM 22.5 (12.5; 41.0) kPa. LSM correlated strongly with HVPG (Spearman’s ρ: 0.725, p < 0.001) and moderately with the model for end-stage liver disease (MELD) score (ρ: 0.459, p = 0.002). LSM, MELD and albumin levels had good prognostic value for decompensation (area under the receiver operated characteristics curve (AUROC) >0.850 for all). LSM predicted (further) decompensation in competing risk regression (subdistribution hazard ratio (SHR): 1.05 (95% confidence interval(CI) 1.03–1.06); p < 0.001), even after adjusting for other factors. An LSM cut-off at 25kPa accurately stratified patients into a low-risk (n = 23, zero events during follow-up) and a high-risk (n = 19; n = 12 (63.2%) developed events during follow-up) group. Conclusions: Patients with HBV-induced CSPH who achieved long-term viral suppression were protected from decompensation, if LSM was <25 kPa. LSM ≥ 25 kPa indicates a persisting risk for decompensation, despite long-term HBV suppression.
Acknowledgment to Reviewers of Journal of Zoological and Botanical Gardens in 2021
Authors:   JZBG Editorial Office
Published: February 2022

Rigorous peer-reviews are the basis of high-quality academic publishing [...]
How Land Finance Drives Urban Expansion under Fiscal Pressure: Evidence from Chinese Cities
Authors: Tong De , Chu Jun , Han Qing , Liu Xuan
Published: February 2022

Urban expansion is commonly attributed to market-oriented forces, while public finance factors connected to government forces, especially land finance, in China’s institutional environment, can provide a more interesting explanation. Using a fixed-effect threshold model, the relationships of land finance and differing local fiscal pressures to urban expansion in China were explored mathematically and empirically. It was found that land finance positively and significantly influences urban expansion. It was also found that the impact of land finance on urban spatial size varies with fiscal pressure. Results suggested that capital flows in the process of urban expansion should be carefully regulated by local governments.
A Review of Crop Husbandry and Soil Management Practices Using Meta-Analysis Studies: Towards Soil-Improving Cropping Systems
Authors: Rietra René , Heinen Marius , Oenema Oene
Published: February 2022

Coherent improvements in crop varieties and crop husbandry and soil management practices are needed to increase global crop production in a sustainable manner. However, these practices are often discussed separately, and as a result there is little overview. Here, we present a database and synthesis of 154 meta-analysis studies related to ten main crop husbandry and soil management practices, including crop type and rotations, tillage, drainage, nutrient management, irrigation and fertigation, weed management, pest management, crop residue management, mechanization and technology, and landscape management. Most meta-analysis studies were related to tillage (55), followed by crop type and rotations (32), nutrient management (25), crop residue management (19), and irrigation and fertigation (18). Few studies were related to landscape management (6) and mechanization and technology (2). In terms of outcome, studies focused on crop yield and quality (81), soil quality (73), and environmental impacts (56), and little on economic effects (7) or resource use efficiency (24). Reported effects of alternative practices, relative to conventional practice, were positive in general. Effect sizes were relatively large for environmental effects (nutrient leaching, greenhouse gas emissions), and small for soil quality (except for soil life) and crop yield. Together, meta-analysis studies indicate that there is large scope for increasing cropland productivity and minimizing environmental impacts. A roadmap is provided for integration and optimization of all ten practices, and recommendations are formulated to address the gaps in meta-analysis studies.