bluuebunny/crossref_metadata_2025 huggingface dataset:
DISPARITAS PEMIDANAAN TERHADAP PEMBALAKAN LIAR (illegal Logging) SERTA DAMPAKNYA TERHADAP PEMBANGUNAN PENEGAKAN HUKUM DI INDONESIA
Authors: Sahyana Yana
Published: March 2023

Artikel ini berkaitan dengan kajian tentang isu-isu mengapa ada disparitas hukuman dalam putusan pengadilan pidana terhadap illegal logging di Indonesia, apa dampak dari disparitas tersebut dalam penegakan hukum terhadap illegal logging di Indonesia dan bagaimana kebijakan penjatuhan hukuman yang harus diambil. dirumuskan untuk mengurangi hukuman dalam putusan pengadilan pidana terhadap pembalakan liar di Indonesia.
Upaya Pemerintah Kabupaten Ciamis Terhadap Perlindungan Hukum Bagi Konsumen Produk Pangan Dalam Kemasan Tanpa Label Halal Pada Usaha Kecil
Authors: Suhendi Asep Hendi , Brata Yat Rospia , Farida Ida , Lubis Fahmi Zulkifli
Published: March 2023

Upaya Pemerintah Kabupaten Ciamis terhadap Perlindungan Hukum Bagi Konsumen Produk Pangan Dalam Kemasan Tanpa Label Halal Pada Usaha Kecil. Permasalahan utama dalam penelitian ini adalah bagaimana perlindungan hukum bagi konsumen produk pangan dalam kemasan tanpa label halal pada usaha kecil. Penelitian ini bertujuan untuk mengetahui bagaimana pengaturan produk pangan berlabel halal dalam kaitannya dengan perlindungan konsumen, dan upaya hukum apa yang dapat dilakukan konsumen dalam memperoleh perlindungan terhadap haknya. Metode penelitian yang digunakan dalam penelitian adalah hukum normatif dengan pendekatan perundang-undangan, dan pendekatan konsep. Informasi didapatkan dari bahan hukum primer, bahan hukum sekunder dan bahan non hukum. Adapun bahan hukum, baik bahan hukum primer, bahan hukum sekunder maupun bahan non hukum diuraikan dan dihubungkan sedemikian rupa, sehingga ditampilkan dalam penulisan yang lebih sistematis untuk menjawab permasalahan yang telah dirumuskan. Dari hasil penelitian tersebut diatas, dapat diperoleh kesimpulan bahwa kewajiban yang harus dilakukan dan dilaksanakan oleh pelaku usaha khususnya usaha kecil. untuk mencantumkan label halal pada kemasan pada setiap produk yang diproduksi sesuai dengan Pasal 8 Ayat (1) huruf h UUPK, dan upaya hukum yang dapat dilakukan konsumen sesuai dengan UUPK yaitu, dapat menyelesaikan sengketa secara langsung kepada pelaku usaha, pengaduan melalui YLKI, Penyelesaian melalui BPOM, melapor ke BPSK dan penyelesaian melalui Peradilan Umum.
Penegakan Hukum Terhadap Tindak Pidana Perzinaan
Authors: Hakim Lukmanul , Aprinisa Aprinisa , Apriliyandra Chiquita
Published: March 2023

Indonesia sebagai negara yang kaya akan budaya dan adat istiadat tentu saja masih menjunjung tinggi norma-norma kehidupan yang diantaranya mencakup adanya norma agama, norma kesusilaan, norma kesopanan dan norma hukum. Meskipun telah ada beberapa norma tersebut, masih tidak cukup untuk membentengi beberapa masyarakat dalam berkehidupan terlebih semasa kini di mana pesatnya perkembangan sosial budaya di Indonesia serta teknologi komunikasi membuat semakin banyak timbulnya perilaku yang tidak sesuai dengan norma yang ada. Permasalahan yang dikaji dalam penelitian ini terkait dengan permasalahan dalam penelitian ini adalah bagaimana penegakan hukum terhadap tindak pidana perzinaan (Studi Putusan Nomor: 1213/Pid.B/2020/PN.Tjk) dan faktor yang menjadi kendala dalam penegakan hukum terhadap tindak pidana perzinaan (Studi Putusan Nomor: 1213/Pid.B/2020/PN.Tjk). Metode penelitian yang digunakan adalah penelitian doktrinal yuridis normatif. Pendekatan yang digunakan penulis adalah pendekatan perundang-undangan dan pendekatan kas. Hasil pembahasan penelitian ini bahwa tindak pidana perzinahan merupakan suatu tindak pidana yang masuk dalam kategori delik aduan. Sehingga yang dapat menuntut atas terjadinya kasus perzinahan tersebut hanyalah pihak yang dirugikan yaitu pasangan sah dari pelaku perzinahan. Namun Sistem pembuktian tindak pidana perzinahan juga kerap menjadi kesulitan tersendiri bagi pihak yang dirugikan karena pelaku perzinahan sehingga aksi penggerebekan pelaku perzinahan merupakan alternatif yang sangat efektif untuk menjerat para pelaku. Banyaknya kasus perzinahan yang semakin hari semakin meningkat namun sangat jarang tersentuh oleh hukum pidana.
HOW THE HUMAN BRAIN LEARNS AND MEMORIES AND HOW IT SHOWS SIMILAR NEURAL PATTERNS TO ITS SOCIAL NETWORK
Authors: Russo Aurora
Published: March 2023

We are continuously faced with a stream of information, which the brain needs to encode and integrate into existing knowledge.  Numerous factors affect this process, such as emotions, neurogenesis, and neuroplasticity. To explain how memory and learning are encoded in the brain, Semon's proposed the existence of engrams cells distributed across the cortex forming a network supporting a given memory, which are activated both during encoding and during recall. It appears that engrams can be either active or silent depending on the stage of memory consolidation. This would be in accordance with the system consolidation theory; however, incongruences in the literature still make it impossible to exclude other alternatives, like the multiple trace theory, as possible explanations. Moreover, it has been found that both learning and memory processes are socially affected. In fact, friendship status, social network features, and hierarchy position have an influence on how people encode information and store them.
NEUROSCIENCES ET ÉDUCATION: QUELLE PERTINENCE POUR L’UNIVERSITÉ MAROCAINE?
Authors: Bouhafs Mounia
Published: March 2023

Les connaissances neuroscientifiques fournissent aujourd’hui suffisamment d'informations sur les mécanismes de l'apprentissage pour modifier les pratiques d'enseignement et de formation. La présente recherche est motivée par la nécessité de vérifier si les enseignants universitaires marocains sont informés de l’apport des neurosciences et s’ils exploitent les connaissances  neuroscientifiques  dans  leurs pratiques pédagogiques pour favoriser les apprentissages, sachant qu’une meilleure compréhension des mécanismes cérébraux d’apprentissage pourrait faciliter la mise en application des connaissances issues de la recherche et ainsi favoriser la réussite scolaire des étudiants..  Une recherche qualitative a été menée dans ce sens. Des entretiens semi-directifs ont été réalisés auprès des enseignants-chercheurs de l’Université Cadi Ayyad. Les résultats obtenus montrent que les neurosciences restent encore boudées dans le contexte universitaire marocain.
THE ROLE OF THE SALIENCE NETWORK AND THE DEFAULT MODE NETWORK IN SECOND LANGUAGE LEARNING
Authors: Juric Ivana
Published: March 2023

L’objectif principal de ce projet était d’appliquer les connaissances en neuroéducation sur les deux réseaux cérébraux, le réseau de saillance (SN) et le réseau de mode par défaut (DMN) et leur importance dans le processus d’apprentissage pour activer et maintenir l’attention et la motivation des étudiants. Suite à l'explication de comment notre cerveau fonctionne lors de l'apprentissage d'une langue seconde, les commentaires que j’ai reçus de la part des étudiants suggèrent qu'ils sont effectivement motivés à participer à mes cours. Cependant, comme il n’y a pas de rétroaction concernant l’attention, je ne peux pas en tirer des conclusions quantitatives. Une grande limite de ce projet est le questionnaire, qui était trop ouvert et n’abordait pas l’aspect de l’attention. Une autre limite de ce projet est que je ne peux pas comparer la motivation et l’attention au début et à la fin du projet. En conséquence, je ne peux pas dire si la forte motivation est effectivement due à mes méthodes d’enseignement ou à l'explication de comment le cerveau fonctionne. Enfin, le temps du projet était trop court pour tirer des conclusions significatives sur le succès des techniques d’enseignement, c’est pourquoi j’ai l’intention de poursuivre ce projet jusqu’à la fin de cette année scolaire et de répéter le questionnaire avec des questions plus précises les années à venir.
ATELIERS RYTHME ET MOUVEMENT POUR ENFANTS AYANT DES TROUBLES DU NEURODÉVELOPPEMENT
Authors: Hedge Nathalie , Castella Nathalie
Published: March 2023

La musique, le rythme et le mouvement apportent beaucoup de plaisir, moteur puissant pour développer le lien parent-enfant, le sentiment d’appartenance au groupe et le développement de compétences motrices, cognitives, communicationnelles et sociales. Les neurosciences en démontrent l’efficacité et apportent des éclairages théoriques sur nos connaissances, nous permettant ainsi d’améliorer notre pratique. Les activités MIO offrent des outils très concrets pour notre travail avec des enfants en âge préscolaire. Elles ont confirmé la pertinence de certaines activités que nous proposions auparavant, et nous ont apporté de nouvelles idées, soutenues par des explications neuroscientifiques. Toutefois, les activités MIO étant parfois compliquées pour le type d’enfants que nous accueillons, nous avons dû les simplifier, voire les remplacer par des activités plus accessibles. Nous avons également mis en place des aménagements adaptés aux besoins des enfants avec TSA : supports visuel, rituels, structure claire, guidance, répétitions, aménagement de l’espace. Ce travail nous a amenées à faire preuve de créativité et à nous remettre en question pour optimiser notre pratique. Nous réalisons que la compétence d’inhibition (principalement « attendre avant d’agir ») a été particulièrement stimulée. Elle est en effet la première compétence à acquérir pour accéder plus tard aux autres fonctions exécutives. Le bilan des ateliers a été réalisé principalement par oral avec les parents, les questionnaires étant compliqués à remplir pour certains d’entre eux (langue, compréhension, disponibilité). Globalement, tous ont mentionné leur plaisir et celui de leur enfant, l’intérêt et l’utilité des activités proposées. Certaines ont été reprises à la maison, soit spontanément par les enfants, soit sur proposition des parents. Les parents ont apprécié d’être intégrés dans un groupe, d’avoir des échanges et de côtoyer d’autres enfants avec des besoins particuliers. Ce travail a confirmé la valeur de nos ateliers, car ils permettent des interactions et des apprentissages renforcés par la dynamique stimulante du groupe. Nous sommes très motivées à poursuivre ce projet et il nous tient à cœur que des familles puissent continuer à en bénéficier. Pour la suite, nous imaginons créer une base de données des activités intéressantes en lien avec notre travail, afin que nos collègues puissent les utiliser dans leurs interventions auprès des familles.
AMÉLIORER LA GESTION COGNITIVE ET ÉMOTIONNELLE D’ENFANTS ET ADOLESCENTS ÂGÉS DE 11 À 14 ANS ATTEINTS DE TROUBLES DU NEURODÉVELOPPEMENT
Authors: Jaccard Merlin Odile
Published: March 2023

Les neurosciences permettent une meilleure connaissance des mécanismes cérébraux en jeu lors de l’apprentissage et soutiennent la pratique de l’enseignant. La dimension dynamique du cerveau permet d’appréhender les difficultés non plus comme une fatalité, mais comme des défis à résoudre puisque la neuroplasticité est possible toute la vie. En effet, les structures cérébrales sont présentes dès la naissance, mais c’est grâce à l’environnement et aux expériences que les connexions neuronales peuvent se développer. Notre rôle en tant que professionnels de l’éducation est donc primordial afin de créer des conditions favorables à l’apprentissage en dépit des vulnérabilités génétiques et environnementales. Beaucoup d’élèves en enseignement spécialisé présentent des troubles du neurodéveloppement et des troubles des apprentissages. Leurs parcours scolaires souvent chaotiques, impactent leur estime d’eux-mêmes et leur confiance en eux. Par ailleurs, leurs difficultés dans les fonctions exécutives ont des répercussions sur leur adaptation et dans leurs relations interpersonnelles. Les émotions prennent une place importante à l’école et influencent toute situation d’apprentissage. Une intervention précoce sur ces fonctions exécutives et la régulation émotionnelle peut être un moyen de prévention des comportements antisociaux à l’adolescence. De plus, des techniques liées à la respiration et à la pleine conscience peuvent diminuer l’anxiété à l’école. La réussite scolaire ne dépend pas seulement des capacités académiques, mais en grande partie des compétences socio-émotionnelles que l’enfant aura pu acquérir. Cette recherche basée sur les apports des neurosciences de l’éducation est une tentative pour permettre aux élèves atteints de troubles du neurodéveloppement d’acquérir des stratégies pour améliorer la gestion de leur comportement émotionnel et cognitif, afin qu’ils puissent construire des liens sociaux positifs et vivre une scolarité épanouie.
Some Features of Form-Building in Modern Rock Music
Authors: Сыров В.Н.
Published: March 2023

Особенности формообразования в рок-музыке рассматриваются в эволюционном аспекте в контексте двух тенденций — имманентных преобразований и привнесения новых принципов извне. Первая связана с трансформацией песенной структуры за счет внутренних ресурсов материала куплета, вторая — с влиянием других форм европейской музыки, предполагающими иные средства развития материала по сравнению с куплетным принципом, и со становлением сквозной песенно-строфической формы, в которой важную роль играет музыкально-драматургический фактор. Различные модификации последней подвергаются в статье специальному рассмотрению. Одна из них характеризуется «равнинным» динамическим рельефом, пребыванием в одном состоянии. Другая представляет постепенное (а порой волнообразное) восхождение к кульминации. А третья образует сложный динамический рельеф, усиленный тематическими контрастами и тональными переходами. Кроме того, отмеченные модификации могут взаимодействовать и давать различные сочетания. Большинство из них неотделимы от вариантности как типа мышления. В качестве аналитического материала привлекаются композиции современного арт- и прогрессив-рока (Yes, Porcupine Tree, Radiohead, Anathema, Muse и других групп).

In this article, features of form-building in rock music are considered in aspect of their evolution in the context of two trends, that is, immanent transformations and introduction of new principles from outside. The first is connected with the transformation of the song composition due to the internal resources of the verse material, the second is connected with the influence of other forms of European music, implying other means of development of material in comparison with the verse-chorus principle, and with the formation of a through-composed song-strophic form, in which the musical-dramaturgical factor plays an important role. Various modifications of the latter are subjected to special consideration in the article. One of them is characterized by a plain dynamic relief. Another modification represents a gradual (sometimes in a wave form) ascent to the culmination. At last, there exists the third variant which is characterized by musical contrasts and a tonal transition. Besides, the modifications can interact with each other and form different combinations. Compositions of modern art and progressive rock (Yes, Porcupine Tree, Radiohead, Anathema, Muse and other groups) are analyzed in the article.
Comparing transferability in neural network approaches and linear models for machine-learning interaction potentials
Authors: Ammothum Kandy Akshay Krishna , Rossi Kevin , Raulin-Foissac Alexis , Laurens Ga ́etan , Lam Julien
Published: March 2023

Atomic simulations using machine learning interatomic potential (MLIP) have gained a lot of popularity owing to their accuracy in comparison to conventional empirical potentials. However, the transferability of MLIP to systems outside the training-set poses a significant challenge. Here, we compare the transferability of three MLIP approaches: i) Neural Network Potentials (NNP), ii) Physical LassoLars Interactions Potential (PLIP) and iii) Linear Potentials with Belher-Parrinello descriptors, trained over a small but diverse configuration of zinc oxide polymorphs. We obtained good agreement between each MLIP models and density functional theory reference results for physical properties including bulk lattice parameters, surface energies, and vibrational density of states. However, the NNP model performed poorly when compared to the other two linear models for the structural optimization of nanoparticles and molecular dynamics simulation of liquid phases, which are systems outside the training-set. While providing less accurate prediction for solid Zinc Oxides phases, both linear models appear more transferable than NNP when testing for nanoscale systems and liquid phases
A process-level perspective of the impact of molecular force fields on the computational screening of MOFs for carbon capture
Authors: Cleeton Conor , Lopes de Oliveira Felipe , Neumann Rodrigo , Farmahini Amir , Luan Binquan , Steiner Mathias , Sarkisov Lev
Published: March 2023

The question we pose in this study is to what extent the ranking of metal organic frameworks (MOFs) for adsorption-based carbon capture, and the selection of top performers identified in PSA process modelling, depends on the choice of the commonly available forcefields. To answer this question, we first generated distributions of CO2 and N2 adsorption isotherms via molecular simulation in 690 MOFs using six typical forcefields: the UFF or Dreiding sets of Lennard-Jones parameters, in combination with partial charges derived from ab initio calculations (DDEC scheme) or by charge equilibration (Qeq an EQeq schemes). We then conducted a systematic uncertainty quantification study using PSA process-level modelling. We observe that: (i) the ranking of MOFs significantly depends on the choice of forcefield; (ii) partial charge assignment is the prevailing source of uncertainty, and that charge equilibration schemes produce results which are inconsistent with ab initio-derived charges; iii) the choice of Lennard-Jones parameters is a considerable source of uncertainty. Our work highlights that is not really possible to obtain material rankings with high resolution using a single molecular modelling approach and that, as a minimum, some uncertainty should be estimated for the performance of MOFs shortlisted using high throughput computational screening workflows. Future prospects for computational screening studies are also discussed.
A2Bn-1PbnI3n+1 (A = BA, PEA; B = MA, n = 1, 2): Engineering Quantum-well Crystals for High Density and Fast Scintillators
Authors: Kuddus-Sheikh Md Abdul , Kowal Dominik , Mahyuddin Muhammad Haris , Cala Roberto , Auffray Etiennette , Witkowski Marcin , Makowski Michal , Drozdowski Winicjusz , Wang Hong , Dujardin Christophe , Cortecchia Daniele , Birowosuto Muhammad Danang
Published: March 2023

Quantum-well (QW) hybrid organic-inorganic perovskite (HOIP) crystals, e.g. A2Pb2X4 (A = BA, PEA, X = Br, I), demonstrated significant potentials as scintillating materials for wide energy radiation detection compared to their individual three-dimensional (3D) counterparts, e. g. BPbX3 (B = MA). Inserting 3D into QW structures resulting new structures namely A2BPb2X7 perovskite crystals and they may have promising optical and scintillation properties towards higher mass density and fast timing scintillators. In this article, we investigate the crystal structure, optical and scintillation properties of iodide-based QW HOIP crystals, A2PbI4 and A2MAPb2I7. A2PbI4 crystals exhibit green and red emission with fastest PL decay time < 1 ns, while A2MAPb2I7 crystals exhibit high mass density of > 3.0 g/cm3, and tunable smaller band gaps < 2.1 eV resulting from quantum and dielectric confinement. We observe that only PEA cation-based A2MAPb2I7 shows emission under X- and γ-ray excitations. We further observe that QW HOIP iodide scintillators exhibit shorter radiation absorption length (~3 cm at 511 keV) and faster decay time component (~0.5 ns) compared to QW HOIP bromide scintillators. We investigate the light yields of both QW HOIP crystals (> 7 photons/keV) at 10 K, while at room temperature are still low > 0.6 photons/keV compared to our previously reported QW bromide crystals (10-40 photons/keV). Thus, promising results of our study on iodide-based QW HOIP scintillators provide the right pathway for further enhancement towards fast-timing applications.
Efficient automatic construction of atom-economical QM regions with point-charge variation analysis
Authors: Brandt Felix , Jacob Christoph
Published: March 2023

The setup of QM/MM calculations is not trivial since many decisions have to be made by the simulation scientist to achieve reasonable and consistent results. The main challenge to be tackled is the construction of the QM region to make sure to take into account all important amino acid residues and exclude less important ones. In our previous work [J. Chem. Theory Comput. 65, 18, 2584–2596 (2022)], we introduced the point charge variation analysis (PCVA) as a simple and reliable tool to systematically construct QM regions based on the sensitivity of the reaction energy with respect to variations of the MM point charges. Here, we assess several simplified variants of this PCVA approach for the example of catechol O-methyltransferase and apply PCVA for another system, the triosephosphate isomerase. Furthermore, we extend its scope by applying it to a DNA system. Our results indicate that PCVA offers an efficient and versatile approach of the automatic construction of atom-economical QM regions, but also identify possible pitfalls and limitations.
Bidirectional Elongation Strategy Using Ambiphilic Radical Linchpin for Modular Access to 1,4-Dicarbonyls via Sequential Photocatalysis
Authors: Matsumoto Akira , Maeda Natsumi , Maruoka Keiji
Published: March 2023

Organic molecules which connect themselves with multiple substrates by sequential C−C bond formations can be utilized as linchpin compounds in multicomponent process. While they are useful for rapidly increasing the molecular complexity, most of the reported linchpin coupling methods rely on the use of organometallic species as strong carbon nucleophiles to form C−C bonds, which narrows the functional group compatibility. Here, we describe a metal-free, radical-mediated coupling approach using a formyl-stabilized phosphonium ylide as a multifunctional linchpin under visible-light photoredox conditions. The present method demonstrates an ambiphilic character of the phosphonium ylide, which serves both as a nucleophilic and an electrophilic carbon-centered radical source. The stepwise and controllable generation of these radical intermediates allows sequential photocatalysis involving two mechanistically distinct radical additions, both of which are initiated by the same photocatalyst in one pot with a high functional-group tolerance. The methodology enables a bidirectional assembly of the linchpin with two electronically differentiated alkene fragments and thus offers rapid and modular access to 1,4-dicarbonyl compounds as versatile synthetic intermediates.
Direct Printing of Helical Metal Arrays by Circularly Polarized Light
Authors: Kim Ji Young , McGlothin Connor , Cha Minjeong , Pfaffenberger Zechariah , Turali Emre Emine , Choi Wonjin , Kim Sanghoon , Biteen Julie , Kotov Nicholas
Published: March 2023

Chiral metasurface with orthogonal nanohelical metal arrays provide strong optical rotation but demands multi-step nanofabrication at low-pressure and/or high-temperature conditions, which is incompatible with many substrates and high-throughput assessment. Submillimeter local photonic patterns with various optical polarization were also hitherto unattainable over the same substrate. Here, we demonstrate direct substrate-tolerant printing of silver nanohelicoids with a locally variable optical activity using circularly polarized light (CPL), producing centimeter-scale chiral metasurface within minutes. The light-illuminated sites on the substrate immersed in an aqueous silver ion solution are activated for heterogeneous nucleation at room temperature. Subsequent CPL-induced asymmetric site-selective deposition and self-assembly of the silver nanoparticles (NPs) sculpt the orthogonal silver helicoids at the interface as one-pot synthesis. The ellipticity and wavelength of the incident photons control the handedness and size of the printed silver helicoids, realizing on-the-fly modulation of optical polarization while printing local patterns. Processing simplicity, high polarization rotation, and fine spatial resolution of the light-driven printing can provide a pathway to the sustainable production of chiral plasmonic metasurfaces, accelerating the development of chiral photonics for health and information technologies.
PepFun 2.0: improved protocols for the analysis of natural and modified peptides
Authors: Ochoa Rodrigo
Published: March 2023

The role of peptides is nowadays relevant in fields such as drug discovery and biotechnology. Computational analyses are required to study their properties and gain insights into rational design strategies. Both natural and modified peptides containing non-natural amino acids require customized tools to run sequence and structure-based studies. PepFun 2.0 is a new version of the python package for the study of natural and modified peptides using a set of modules to analyze the sequence and structure of the molecules. PepFun 2.0 comprises five main modules for different tasks such as sequence alignments, prediction of properties, generation of conformers, modification of structures, detection of protein-peptide interactions, and extra functions to include peptides containing non-natural amino acids. The code and tutorial are available at: https://github.com/rochoa85/PepFun2
Fluorescent Ligand Enables Target Engagement Studies for the Intracellular Allosteric Binding Site of the Chemokine Receptor CXCR2
Authors: Huber Max , Wurnig Silas , Weiler Corinna , Merten Nicole , Toy Lara , Kostenis Evi , Hansen Finn , Schiedel Matthias
Published: March 2023

Fluorescently labeled ligands are versatile molecular tools to study G protein-coupled receptors (GPCRs) and can be used for a range of different applications, including bioluminescence resonance energy transfer (BRET) assays and fluorescence microscopy. Herein, we report the structure-based development of a fluorescent ligand targeting the intracellular allosteric binding site (IABS) of the CXC chemokine receptor 2 (CXCR2), a class A GPCR that has been pursued as a drug target in oncology and inflammation. Starting from the co-crystallized intracellular CXCR2 antagonist 00767013 (1), a tetramethylrhodamine (TAMRA)-labeled CXCR2 ligand was designed, synthesized, and tested for its suitability as fluorescent reporter to probe binding to the IABS of CXCR2. By means of these studies, we developed Mz438 (9) as a high affinity fluorescent CXCR2 ligand, enabling cell-free as well as cellular NanoBRET-based binding studies in a non-isotopic and high-throughput manner. Further, we show that 9 can be used as a tool to visualize intracellular target engagement for CXCR2 via fluorescence microscopy. Thus, our small molecule-based fluorescent CXCR2 ligand 9 represents a promising tool for future studies of CXCR2 pharmacology.
How do donor and acceptor substituents change the photophysical and photochemical behavior of dithienylethenes? The search for a water-soluble visible-light photoswitch
Authors: Qiu Sili , Frawley Andrew , Leslie Kathryn , Anderson Harry
Published: March 2023

Dithienylethenes are a type of diarylethene and they constitute the most widely studied class of photoswitches, yet there have been no systematic studies of how electron-donor or -acceptor substituents affect their properties. Here we report eight dithienylethenes bearing push-push,pull-pull and push-pull substitution patterns with different lengths of conjugation in the backbone and investigate their photophysical andphotochemical properties. Donor-acceptor interactions in the closed forms of push-pull dithienylethenes shift their absorption spectra into the near-infrared (lmax 800 nm). The push-pull systems also exhibit low quantum yields for photochemical electrocyclization, and computational studies indicate that this can be attributed to stabilization of the parallel, rather than anti-parallel, conformations. The pull-pull systems have the highest quantum yields for switching in both directions, ring-closure and ring-opening. The chloride salt of a pull-pull DTE, with alkynes on both arms, is the first water-soluble dithienylethene that can achieve >95% photostationary state distribution in both directions with visible light. It has good fatigue resistance: in aqueous solution on irradiation at 365 nm, the photochemical quantum yields for switching and decomposition are 0.15 and 2.6 x 10–5 respectively, indicating that the molecule could undergo >5000 switching cycles before decomposition. These properties make it a promising candidate for biological applications such as super-resolution microscopy and photopharmacology.
A scalable crystal representation for reverse engineering of novel inorganic materials using deep generative models
Authors: Tewari Abhishek , Bajpai Rochan , Shukla Atharva , Kumar Janish
Published: March 2023

The efficient search for crystals with targeted properties is a significant challenge in materials discovery. The rapidly growing field of materials informatics has so far primarily focused on the application of AI/ML models to predict the properties of known crystals from their fundamental and derived properties as descriptors. In the last few years, deep learning-based approaches have spawned a slew of innovative data-driven materials research applications. Materials scientists have used these techniques for the reverse engineering of crystal structures for target applications. However, one of the challenges has been the representation of the crystal structures in the machine readable format. Proposed representations in the literature lack in generality and scalability. In this paper, we train a conditional variational autoencoder with a scalable and invertible representation along with the elemental properties of the constituents as descriptors to inverse-design new crystal structures with specified attributes. When targeting formation energy, we show that our model predicts structures that are not in the complete OQMD database. Finally, we use first-principles density functional theory calculations to validate our findings and show that the developed model is able to generate novel crystal structures for targeted property, i.e. formation energy in this case.
H4K20me1 plays a dual role in transcriptional regulation of regeneration and axis patterning inHydra
Authors: Gungi Akhila , Saha Shagnik , Pal Mrinmoy , Galande Sanjeev
Published: March 2023

The evolution of the first body axis in the animal kingdom and its extensive ability to regenerate makesHydra, a Cnidarian, an excellent model system for understanding the underlying epigenetic mechanisms. We identify that monomethyltransferase SETD8 is critical for regeneration inHydrabecause of its conserved interaction with β-catenin to fine-tune the associated gene regulatory network. Inhibition of SETD8 activity abolishes head and foot regeneration inHydra. Furthermore, we show that H4K20me1, the histone mark imparted by SETD8, colocalizes with the transcriptional activation machinery locally at the β-catenin-bound TCF/LEF-binding sites on the promoters of head-associated genes, marking an epigenetic activation mode. In contrast, genome-wide analysis of the H4K20me1 occupancy revealed a negative correlation with transcriptional activation. We propose that H4K20me1 acts as a general repressive histone mark in Cnidaria and describe its dichotomous role in transcriptional regulation inHydra.
Cell death and barrier disruption by clinically used iodine concentrations
Authors: Steins Anne , Carroll Christina , Choong Fui Jiun , George Amee J , He Jin-Shu , Parsons Kate M , Feng Shouya , Man Si Ming , Kam Cathelijne , van Loon Lex M , Poh Perlita , Ferreira Rita , Mann Graham J , Gruen Russell L , Hannan Katherine M , Hannan Ross D , Schulte Klaus-Martin
Published: March 2023

Povidone-iodine (PVP-I) inactivates a broad range of pathogens. Despite its widespread use over decades, the safety of PVP-I remains controversial. Its extended use in the current SARS-CoV-2 virus pandemic urges the need to clarify safety features of PVP-I on a cellular level. Our investigation in epithelial, mesothelial, endothelial, and innate immune cells revealed that the toxicity of PVP-I is caused by diatomic iodine (I2), which is rapidly released from PVP-I to fuel organic halogenation with fast first-order kinetics. Eukaryotic toxicity manifests at below clinically used concentrations with a threshold of 0.1% PVP-I (wt/vol), equalling 1 mM of total available I2. Above this threshold, membrane disruption, loss of mitochondrial membrane potential, and abolition of oxidative phosphorylation induce a rapid form of cell death we propose to term iodoptosis. Furthermore, PVP-I attacks lipid rafts, leading to the failure of tight junctions and thereby compromising the barrier functions of surface-lining cells. Thus, the therapeutic window of PVP-I is considerably narrower than commonly believed. Our findings urge the reappraisal of PVP-I in clinical practice to avert unwarranted toxicity whilst safeguarding its benefits.
Filopodia-like protrusions of adjacent somatic cells shape the developmental potential of oocytes
Authors: Crozet Flora , Letort Gaëlle , Bulteau Rose , Da Silva Christelle , Eichmuller Adrien , Tortorelli Anna Francesca , Blévinal Joséphine , Belle Morgane , Dumont Julien , Piolot Tristan , Dauphin Aurélien , Coulpier Fanny , Chédotal Alain , Maître Jean-Léon , Verlhac Marie-Hélène , Clarke Hugh J , Terret Marie-Emilie
Published: March 2023

The oocyte must grow and mature before fertilization, thanks to a close dialogue with the somatic cells that surround it. Part of this communication is through filopodia-like protrusions, called transzonal projections (TZPs), sent by the somatic cells to the oocyte membrane. To investigate the contribution of TZPs to oocyte quality, we impaired their structure by generating a full knockout mouse of the TZP structural component myosin-X (MYO10). Using spinning disk and super-resolution microscopy combined with a machine-learning approach to phenotype oocyte morphology, we show that the lack ofMyo10decreases TZP density during oocyte growth. Reduction in TZPs does not prevent oocyte growth but impairs oocyte-matrix integrity. Importantly, we reveal by transcriptomic analysis that gene expression is altered in TZP-deprived oocytes and that oocyte maturation and subsequent early embryonic development are partially affected, effectively reducing mouse fertility. We propose that TZPs play a role in the structural integrity of the germline–somatic complex, which is essential for regulating gene expression in the oocyte and thus its developmental potential.
El espectro autista claves de comprensión para familias, cuidadores y docentes
Authors: Sánchez Fernando Andrade , Ahumada Rodríguez Ana Abigail , Martínez Cárdenas Mónica Patricia , Polanía Sánchez Yira Betsie
Published: March 2023

Las letras que iniciarás a leer, más que un libro, son un diálogo directo con padres, cuidadores y familiares de personas que manifiestan sus formas de ser y estar en el mundo de maneras no convencionales y que, lejanos a los estándares, configuran retos de atención y formación llenos de incógnitas y nuevas rutas aun por transitar. Aunque una de las maneras posibles para abordar esta temática es enunciar a los sujetos como personas con discapacidad, la obra El Espectro Autista, claves de comprensión para familias, cuidadores y docentes, aborda la diversidad de capacidades con la firme intención de provocar ejercicios de comprensión, basados en las diversas posibilidades de ser capaces indistintamente de las sospechas diagnósticas y las formalidades médicas al respecto.
Treatment Of Environmental Issues in Leading Pakistani Dailies
Authors: Ishaq Nasim , Ejaz Ghazala Kanwal , Noor
Published: March 2023

Purpose: Environmental issues are contemplated as the most frightening issues that occurred due to climate change. These environmental issues are disturbing the whole world including living species. Pakistan is also facing issues regarding environmental changes. Pakistan is considered the 7th most susceptible country in terms of environmental disasters.

Methodology: This research work is conducted to analyze and examine the role of news media in the coverage of environmental issues and the basic purpose of this study is to evaluate the “media representation of environmental issues” as discussed in mainstream English newspapers of Pakistan (Dawn and The News). The content analysis is used to know the frequency and direction of environmental issues. The universe of the study contains textual content of print media including news stories and editorials.

Findings: It is concluded that The News, gives more coverage to environmental issues as compared to Dawn newspaper in terms of news stories and editorials.

Implications: The media should play a substantial role in addressing environmental problems faced by Pakistan, as people rely frequently on media.
PENGARUH MEDIA PEMBELAJARAN SOFTWARE PROTEUS TERHADAP HASIL BELAJAR SISWA PADA MATA PELAJARAN PENERAPAN RANGKAIAN ELEKTRONIKA KELAS XI DI SMK NEGERI 1 JETIS
Authors: Maniar Tiara , Rusimamto Puput Wanarti , Sulistyo Edy , Fransisca Yulia
Published: March 2023

The research was conducted in SMK Negeri 1 Jetis where the learning process requires media so that students can learn independently to support the activities of the practicum. This study uses a learning media that the software proteus. The purpose of this research is: (1) determine differences in student learning outcomes before the application and after the application of learning media software proteus, (2) determine the response of students to the eye pelejaran the application of the electronic circuit. The method of research is Pre-Experimental Design with the shape of the One-Group Pretest-Posttest Design. The technique of data analysis using the t test to determine differences in learning outcomes and student responses using a sheet of the questionnaire. The validation sheet filled by two faculty of Electrical Engineering and one teachers of SMK. This research resulted in the discussion, namely: (1) Differences in learning outcomes of students by using the t test obtained value of 0.000 and the level of significant was set at 0.05 so that the value of 0,000<0,05 means that H0 is rejected and H1 is accepted. So there is a significant difference between the learning outcomes of students before and after the application of learning media software proteus. (2) the Response of students to obtain the average worth 82% of which consists of 3 aspects, namely aspects of interest, the material and the suitability of the efforts of the subjects. So the average is classified in the category of very good for the students ' response to the subjects. Learning Media can be used as effective media so that students do not feel bored with the material being taught.
PENGEMBANGAN TRAINER LANDSLIDE SMART MITIGATION (LSM) SEBAGAI EDUKASI MITIGASI DINI TANAH LONGSOR DI SMA NEGERI 1 PULUNG
Authors: Pratama Handyesa Dika , Sulistiyo Edy , Baskoro Farid , Nurhayati Nurhayati
Published: March 2023

Improving the quality of education and learning activities in the aspect of disaster mitigation, educational institutions are an important means to help provide students with an understanding of the importance of natural disaster mitigation. Improving the quality of disaster mitigation education can be done by providing students with learning media to improve their understanding of mitigating natural disasters that will occur. The purpose of making trainers disaster mitigation as learning media is an effort to support students' understanding of the importance of natural disaster mitigation. The use of the research & development ADDIE method in this study aims to develop learning media to educate students about disaster mitigation. The data comparison process was carried out using the results of the pretest and posttest by analyzing the learning outcomes of 32 students of SMA Negeri 1 Pulung on geography subjects about landslide disaster mitigation using LSM trainer learning media. Development results Trainer landslide smart mitigation (LSM) obtained a level of validity with average score trainers of 90.54% for, job sheets 87.50% for, and 88.89% for questions, meaning that the research product is very valid to be implemented as a learning medium. Based on analysis of test for normality Kolmogorov-Smirnov gain score significance pretest 0.200 and score significance posttest 0.164 and the results of the t-test analysis obtained a score significance (2-tailed) is 0.000, so the trainer LSM, worksheets, and questions can be said to be effective as a means of support. The practicality of trainers LSM and job sheets are known from the answers to the questionnaire responses of 32 students with an average score of 84.76%, meaning that learning using trainers LSM and job sheets is very practical as a learning medium.
PENGEMBANGAN TRAINER INSTALASI PENERANGAN LISTRIK 3 FASA GEDUNG BERTINGKAT BERBASIS “SMART BUILDING” PADA MATA PELAJARAN INSTALASI PENERANGAN LISTRIK KELAS XI TITL DI SMKN 1 DRIYOREJO
Authors: Putra Alfredo Arianto Permana , Munoto Munoto , Wrahatnolo Tri , Aribowo Widi
Published: March 2023

Trainer adalah salah satu media penunjang pembelajaran yang efektif dalam membantu proses belajar, realitasnya belum semua sekolah memiliki trainer. Penelitian ini bertujuan menghasilkan trainer dan job sheet instalasi penerangan listrik 3 fasa gedung bertingkat berbasis smart building yang layak digunakan, dilihat dari kevalidan, kepraktisan, dan keefektifan. Penelitian ini dilakukan melalui Research and Development (R & D), dan uji coba penelitian ini menggunakan desain penelitian One Shot-Case Study yang dilakukan di SMK Negeri 1 Driyorejo Gresik. Subjek penelitian ini adalah 24 peserta didik kelas XI TITL-1. Data validitas trainer dan job sheet diperoleh dari hasil penilaian tiga ahli menggunakan lembar validasi; data kepraktisan trainer dan job sheet diperoleh dari respon guru dan peserta didik melalui angket respon, dan keefektifan diperoleh dari data hasil belajar peserta didik menggunakan instrumen post test, lembar observasi dan tes kinerja proyek. Teknik analisis data pada penelitian ini menggunakan analisis statistik deskriptif dan one sample t-test. Didapatkan hasil penelitian menunjukkan rating validitas trainer 83 %, 85% dan job sheet 85,77% >80% (sangat valid). Rating respon guru 88, 89% dan peserta didik 86,92% > 80% (sangat praktis). Hasil belajar peserta didik rerata 84,21 (sangat efektif) dan berbeda signifikan dibandingkan nilai KKM>75. Sehingga dapat disimpulkan, trainer dan jobsheet yang dihasilkan sangat layak digunakan dalam pembelajaran instalasi penerangan listrik gedung bertingkat berbasis smart building. Hasil penelitian dapat dipakai pertimbangan kepala sekolah SMK untuk mendorong, memotivasi, dan memfasilitasi guru merealisasikan pembelajaran dan evaluasi hasil belajar dilengkapi trainer dan jobsheet HOTS.
PENGEMBANGAN TRAINER DAN JOBSHEET PENGENDALI MOTOR LISTRIK 3 FASA MENGGUNAKAN RASPBERRY PI DENGAN INTERFACE APLIKASI TELEGRAM PADA MATA PELAJARAN INSTALASI MOTOR LISTRIK DI SMK NEGERI 1 SIDOARJO
Authors: Huda Mohamad Syamsul , Joko Joko , Rijanto Tri , Wrahatnolo Tri
Published: March 2023

Latar belakang dari penelitian ini berdasarkan hasil observasi saat pelaksanaan Pengenalan Lapangan Persekolahan di SMK Negeri 1 Sidoarjo, diketahui pembelajaran masih dilaksanakan secara konvensional, masih menggunakan media trainer yang sudah lama dan belum mengacu pada standar Peraturan Umum Instalasi Listrik terbaru pada mata pelajaran Instalasi Motor Listrik. Penelitian ini bertujuan untuk mengembangkan produk berupa trainer dan jobsheet pengendali motor listrik 3 fasa menggunakan raspberry pi dengan interface aplikasi telegram yang layak digunakan meliputi validitas, kepraktisan, dan keefektifan. Uji coba penelitian ini menggunakan teknik simple random sampling di mana subjek adalah 20 peserta didik kelas XI TITL 1 program keahlian Teknik Instalasi Tenaga Listrik semester genap tahun pelajaran 2020/2021. Penelitian ini menggunakan metode Research and Development, dalam bentuk One Group Pretest–Postest Design. Dari penelitian ini diperoleh hasil kevalidan dengan nilai rerata sebesar 89,06%, maka produk penelitian ini dikategorikan sangat valid digunakan. Hasil kepraktisan dari hasil analisis respon guru sebesar 86,36% dan hasil analisis respon peserta didik sebesar 87,90%, maka produk penelitian ini dikategorikan sangat praktis digunakan. Hasil keefektifan diperoleh dari hasil belajar peserta didik berdasarkan perhitungan analisis uji-t diperoleh hasil (-2 tailed) 0,000. Hasil tersebut menunjukan bahwa nilai 0,000 lebih kecil dari (<) 0,05. Untuk hasil thitung sebesar (45,042) sedangkan ttabel sebesar 2,093 dengan df = 19. Karena thitung (>) ttabel sehingga dapat dikatakan sangat efektif diterapkan pada mata pelajaran Instalasi Motor Listrik di SMK Negeri 1 Sidoarjo. Hasil penelitian ini dapat berkontribusi sebagai masukan bagi guru SMK dalam memperbaiki kualitas pembelajaran dengan menerapkan media trainer dan jobsheet.
PENGEMBANGAN MODUL MIKROKONTROLLER CODEVISION AVR MENGGUNAKAN PERANGKAT LUNAK PROTEUS PROFESSIONAL PADA MATA PELAJARAN MIKROPROSESSOR DAN MIKROKONTROLLER UNTUK SISWA KELAS XI TEI SMKN 1 JETIS
Authors: Purnama Rizki Dwi Putra , Harimurti Rina , Widodo Arif , Rahmadian Reza
Published: March 2023

Education is a place for the formation of a quality generation of the nation. Selection of learning media appropriately can increase the quality of learning in students. Microprocessors and microcontrollers are subjects that study software and hardware on computers The purpose of this study was to develop a codevision AVR microcontroller module using professional proteus software in microprocessor and microcontroller subjects for class students XI TEI SMKN 1 Jetis. In this research, the writer used research and development method and also gave pretest and posttest to 30 students. Retrieval of data with module validation sheets and questions that were validated by 2 lecturers majoring in Electrical Engineering and 1 teacher SMKN 1 Jetis which gets a rating of 84.3% for the module and 89.1% for the questions so that it produces a very valid media. Meanwhile, for the results of the analysis of the normality test using SPSS, the sig value is 0.200 for the pretest and 0.200 for the posttest which is normally distributed. In the T test using SPSS, it was obtained a significance value (2-tailed) of 0.000, so it was stated that there was a significant effect on the differences in the treatment given to each variable.