bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of a VR Stylus with a Controller, Hand Tracking, and a Mouse for Object Manipulation and Medical Marking Tasks in Virtual Reality
Authors: Rantamaa Hanna-Riikka , Kangas Jari , Kumar Sriram Kishore , Mehtonen Helena , Järnstedt Jorma , Raisamo Roope
Published: February 2023

In medical surgery planning, virtual reality (VR) provides a working environment, where 3D images of the operation area can be utilized. VR allows 3D imaging data to be viewed in a more realistic 3D environment, reducing perceptual problems and increasing spatial understanding. In the present experiment, we compared a mouse, hand tracking, and a combination of a VR stylus and a grab-enabled VR controller as interaction methods in VR. The purpose was to investigate the suitability of the methods in VR for object manipulation and marking tasks in medical surgery planning. The tasks required interaction with 3D objects and high accuracy in the creation of landmarks. The combination of stylus and controller was the most preferred interaction method. According to subjective results, it was considered as the most appropriate because it allows the manipulation of objects in a way that is similar to the use of bare hands. In the objective results, the mouse interaction method was the most accurate.
Green Leaf Volatile Function in Both the Natural Defense System of Rumex confertus and Associated Insects’ Behavior
Authors: Piesik Dariusz , Łyczko Jacek , Krawczyk Krzysztof , Gantner Magdalena , Bocianowski Jan , Ruzsanyi Veronika , Mayhew Chris A.
Published: February 2023

Rumex confertus is a rhizomatous, invasive, and difficult to control plant. Nevertheless, for sustainable agriculture, studies to biologically control R. confertus continue. Towards this, considerable attention has been devoted to investigating the emission of a wide array of volatile organic compounds (VOCs) from herbivore-damaged plants, which are known to induce protection measures in neighboring, undamaged plants. The goals of this study are to (1) determine if the profile of green leaf volatiles (GLVs), which are organic compounds naturally produced by undamaged plants, is similar to that provoked by the chemical stimulants Z-jasmone (ZJA) and dihydrojasmone (DJA), (2) establish if the Apion miniatum beetle’s reproductive choices are influenced by their sex and mating status, and (3) assess if chemically stimulated GLV emissions can be used as signals to attract pests to R. confertus for biological control purposes. Synthetic forms of naturally produced Z-jasmone (ZJA) and dihydrojasmone (DJA), which can act as either an attractant or a repellent of various species of insects, were used to treat R. confertus. In olfactory analysis, the behavioral responses of individual insects to mated and unmated insects and to two GLV blends were evaluated. It was found that unmated male insects were fairly equally divided between going for food (Y-tube olfactometer arm with a GLV blend) and opportunities for reproduction (Y-tube olfactometer arm with unmated females). However, an increase in the GLV blend concentration attracted the insects. Meanwhile, unmated females were definitely indifferent to food and, independent of the GLV blend dose, were more interested in reproduction. Mated males, even with weak feed stimuli, increased their reproduction activity, in opposition to mated females. We provide evidence that synthetic GLV blends can be used to attract predators, making their use an effective means to biologically control R. confertus. The idea of applying synthetic GLVs as a biological control is based on the insects’ mutual relationships, which work as follows: (i) mated males fully invade the weed, (ii) reproduction-driven females follow the mated males to R. confertus, and (iii) the unmated males follow the females with the purpose to reproduce. Therefore, all insect groups (mated and unmated males and females) can be induced to invade the weed. Upon feeding, the insects will damage the generative organs of R. confertus. We propose that the use of chemical stimulants to increase GLV emissions can be effectively used for weed (here R. confertus) control by attracting a plant pest (here A. miniatum).
Evaluation of the Robustness for Integrated Production Scheduling and Maintenance Planning Problem
Authors: Ba Zhiyong , Yuan Yiping
Published: February 2023

In this paper, we consider the surrogate measures of robustness for the integration of production scheduling and preventive maintenance planning problem with stochastic machine failures. First, the influence of two categories of maintenance on operation completion time is analyzed. Since it does not come with an exact solution, a novel measure algorithm for evaluating two types of robustness simultaneously is proposed, based on the internal relationships among the scheduling structure, the number and sequence of preventive maintenance activities, the probability and downtime of failures, and the expected completion time of operation. Extensive experiments are conducted on 19 benchmark problems with random machine breakdowns. Experimental results first show that the correlation between our algorithm and Monte Carlo simulation in two robustness indicators is above 99%, and the former computes much faster than the latter. A thorough comparison is made with the other three surrogate robustness measures, which further proves the accuracy of our algorithm. Additional experiments also confirm the benefits by integrating preventive maintenance.
Monitoring of Engineered Stones Used in Artwork Reproductions: Mechanical Characterization by Laser Vibrometry
Authors: Arciniegas Andres , Martinez Loïc , Serfaty Stéphane , Wilkie-Chancellier Nicolas
Published: February 2023

Many museums have been producing reproductions for several years to replace artworks weakened by outdoor exhibition. Among these, in order to imitate the original aesthetic, the French consortium Réunion des Musées Nationaux–Grand Palais has chosen to work from large-format marble sculpture molds to complex composite materials based on resins comprising mineral fillers. However, similar to the original works of art, these reproductions age and deteriorate due to constant outdoor exposure. For this reason, current research focuses on the preventive conservation and monitoring of the structural health of these reconstructed objects. The goal of this paper was to study the resin/mineral powder composite materials used to produce cultural heritage reproductions of sculptures. This work is oriented toward a comparison of the mechanical properties of composite materials used in the replacement of cultural heritage sculptures (for instance, in the Garden of the Palace of Versailles or the Rodin Museum). The objectives were to first characterize the physical and mechanical properties of these materials in order to identify the most suitable material for cultural heritage reproduction, and secondly, to propose a method with minimal contact that obtained equivalent information as analyses performed with conventional ultrasonic techniques. These nondestructive evaluation techniques could be used for laboratory and in situ analyses. Samples of different polymer/mineral powder filler compositions were analyzed by compressional, shear and surface waves, generated by a 1 MHz center frequency ultrasonic transducer. Firstly, the measurements made it possible to evaluate the velocities of the bulk acoustic waves and extract the Young’s modulus of each tested material. Secondly, in order to have minimal contact with the analyzed structure, a laser interferometry system was used to detect waves at the surface and follow their propagation. The results clearly showed the possibility of using this technique to extract mechanical characteristics of composite materials, allowing for selection of material for the reproduction of large-format statues. For different types of polymer resins, the ability of ultrasonic analysis to track the impact of rock powder (marble or slate) on the mechanical properties of these synthetic materials was clearly observed, proving that this technique holds promise for monitoring the structural health of large-format artwork.
Potential of Laurel (Laurus nobilis L.) Leaf Polyphenols for Modulation of Body Composition
Authors: Dobroslavić Erika , Elez Garofulić Ivona , Ilich Jasminka Z.
Published: February 2023

Due to increased life spans and senescence in society, there is a growing need for supplements that enable healthy aging. Aging is accompanied by changes in body composition such as loss of bone and muscle mass and increase/redistribution of adipose tissue. These changes may, at least in part, be alleviated by exercise, adequate diet and possibly some natural adjuvants. Laurel (L. nobilis L.) leaves are rich in secondary metabolites, especially polyphenols (mostly flavonols, phenolic acids and flavones) which have recently showed potential for modulation of body composition that could allow healthier aging. Therefore, the aim of this paper was to evaluate the potential of laurel leaf polyphenols for modulating body composition. We searched the literature available on the PubMed electronic database related to the main polyphenolic representatives of laurel leaf extracts (namely, kaempferol, hydroxycinnamic acids and apigenin) and their modulation of bone, skeletal muscle and adipose tissue. The search yielded 135 articles showing that the main laurel leaf polyphenols exhibit various positive effects on bones, skeletal muscle and adipose tissue. These effects could ameliorate metabolic disorders related to modern lifestyles and result in an improvement of body composition and function, resulting in healthier aging, but more evidence-based research in humans is needed.
A Cloud Intrusion Detection Systems Based on DNN Using Backpropagation and PSO on the CSE-CIC-IDS2018 Dataset
Authors: Alzughaibi Saud , El Khediri Salim
Published: February 2023

Cloud computing (CC) is becoming an essential technology worldwide. This approach represents a revolution in data storage and collaborative services. Nevertheless, security issues have grown with the move to CC, including intrusion detection systems (IDSs). Intruders have developed advanced tools that trick the traditional IDS. This study attempts to contribute toward solving this problem and reducing its harmful effects by boosting IDS performance and efficiency in a cloud environment. We build two models based on deep neural networks (DNNs) for this study: the first model is built on a multi-layer perceptron (MLP) with backpropagation (BP), and the other is trained by MLP with particle swarm optimization (PSO). We use these models to deal with binary and multi-class classification on the updated cybersecurity CSE-CIC-IDS2018 dataset. This study aims to improve the accuracy of detecting intrusion attacks for IDSs in a cloud environment and to enhance other performance metrics. In this study, we document all aspects of our experiments in depth. The results show that the best accuracy obtained for binary classification was 98.97% and that for multi-class classification was 98.41%. Furthermore, the results are compared with those from the related literature.
Free-Breathing and Ungated Cardiac MRI Reconstruction Using a Deep Kernel Representation
Authors: Zou Qing , Ahmed Abdul Haseeb , Dzelebdzic Sanja , Hussain Tarique
Published: February 2023

Free-breathing and ungated cardiac MRI is a challenging problem due to the cardiac motion and respiration motion, which are not tracked. In this work, we propose an unsupervised deep kernel method for reconstructing real-time free-breathing and ungated cardiac MRI from highly undersampled k-t space measurements. We propose implementing the feature map and kernel function in the kernel method using CNNs. The parameters of the CNNs are learned from specific-subject data directly. Comparisons with state-of-the-art kernel methods show improved performance of the proposed deep kernel method.
A Multi-Objective Optimization Method of a Mobile Robot Milling System Construction for Large Cabins
Authors: Wen Ke , Zhang Zhiqiang , Zhang Jiabo , Zhang Xiaohui , Chen Tao , Gao Xin , Zhang Wen
Published: February 2023

Constructing mobile robot milling systems with multiple mounting surfaces for large cabins still has several unsolved issues, such as huge economic and time costs, unpredictable milling accuracy and milling time. Hence, a multi-objective optimization method for constructing a mobile robot milling system of large cabins is proposed in the current paper. Firstly, mathematical models of constructing the system and the optimization objective function are established. Thereafter, a multi-objective optimization method for the mobile robot milling system construction based on NSGA-II (Fast Non-dominated Sorting Genetic Algorithm) is proposed. Finally, feasibility and validity of the proposed method are verified through comparing the optimization result with two practical mobile robot systems. Results show that the proposed method is able to estimate different combinations’ milling accuracy, cost and time consumption.
Relation between Muscle Force of Knee Extensors and Flexor Muscles and Sleep Quality of Women Soccer Players: A Pilot Study
Authors: Rusu Ligia , Ungureanu Dobre Aurora , Chivaran Alexandru Gabriel , Marin Mihnea Ion
Published: February 2023

Physical performance in sport performance such as football is influenced by sleep quality, but there are only a few studies about this and the behaviour of muscle force at knee flexors and extensors. The aim of this study is to make an analysis of sleep, an analysis of muscle force parameters (flexors and knee extensors), and a study of the correlation between sleep quality and muscle force. The study includes 18 junior women footballers, average age 14.75 years old, who participated in a training programme of 90 min, three times/week. The evaluation includes sleep quality evaluation using the sleep quality scale (SQS), sleepiness evaluation using the Cleveland Adolescent Sleepiness Questionnaire (CASQ), and muscle force evaluation using dynamometry for knee flexor and extensor muscles. We recorded maximal muscle force, medium maximal muscle force, and duration of maintained muscle force. We observed that more than 83% of subjects scored below 50% on the maximal SQS score and all participants scored less than 40 points (50%) on the CASQ’s maxim score, meaning a small level of sleepiness. With regard to muscle force, left–right symmetry for medium maximal force shows a predominance of the right-hand side and the value was 1.64%, for knee extensors and for knee flexors the difference was 7.58%, meaning that for knee extensors the symmetry is better than that of knee flexors. Statistical analysis regarding muscle parameters shows that there are no significant statistical differences between the left- and right-hand sides. Because the duration of the maintained maximal muscle force could indicate muscle fatigue, we observed that there was no link, and that sleep quality or sleepiness do not influence muscle fatigue. Our research concludes that muscle asymmetry of the left–right side of knee extensor and flexor muscles is minimal and does not correlate with sleep quality or sleepiness. There is no statistical correlation between muscle force parameters and sleep quality.
Evaluation of qPCR to Detect Shifts in Population Composition of the Rhizobial Symbiont Mesorhizobium japonicum during Serial in Planta Transfers
Authors: Quides Kenjiro W. , Lee Yoobeen , Hur Teresa , Atamian Hagop S.
Published: February 2023

Microbial symbionts range from mutualistic to commensal to antagonistic. While these roles are distinct in their outcome, they are also fluid in a changing environment. Here, we used the Lotus japonicus–Mesorhizobium japonicum symbiosis to investigate short-term and long-term shifts in population abundance using an effective, fast, and low-cost tracking methodology for M. japonicum. We use quantitative polymerase chain reaction (qPCR) to track previously generated signature-tagged M. japonicum mutants targeting the Tn5 transposon insertion and the flanking gene. We used a highly beneficial wild type and moderately beneficial and non-beneficial mutants of M. japonicum sp. nov. to demonstrate the specificity of these primers to estimate the relative abundance of each genotype within individual nodules and after serial transfers to new hosts. For the moderate and non-beneficial genotypes, qPCR allowed us to differentiate genotypes that are phenotypically indistinguishable and investigate host control with suboptimal symbionts. We consistently found the wild type increasing in the proportion of the population, but our data suggest a potential reproductive trade-off between the moderate and non-beneficial genotypes. The multi-generation framework we used, coupled with qPCR, can easily be scaled up to track dozens of M. japonicum mutants simultaneously. Moreover, these mutants can be used to explore M. japonicum genotype abundance in the presence of a complex soil community.
The Structure of Evolutionary Model Space for Proteins across the Tree of Life
Authors: Scolaro Gabrielle E. , Braun Edward L.
Published: February 2023

The factors that determine the relative rates of amino acid substitution during protein evolution are complex and known to vary among taxa. We estimated relative exchangeabilities for pairs of amino acids from clades spread across the tree of life and assessed the historical signal in the distances among these clade-specific models. We separately trained these models on collections of arbitrarily selected protein alignments and on ribosomal protein alignments. In both cases, we found a clear separation between the models trained using multiple sequence alignments from bacterial clades and the models trained on archaeal and eukaryotic data. We assessed the predictive power of our novel clade-specific models of sequence evolution by asking whether fit to the models could be used to identify the source of multiple sequence alignments. Model fit was generally able to correctly classify protein alignments at the level of domain (bacterial versus archaeal), but the accuracy of classification at finer scales was much lower. The only exceptions to this were the relatively high classification accuracy for two archaeal lineages: Halobacteriaceae and Thermoprotei. Genomic GC content had a modest impact on relative exchangeabilities despite having a large impact on amino acid frequencies. Relative exchangeabilities involving aromatic residues exhibited the largest differences among models. There were a small number of exchangeabilities that exhibited large differences in comparisons among major clades and between generalized models and ribosomal protein models. Taken as a whole, these results reveal that a small number of relative exchangeabilities are responsible for much of the structure of the “model space” for protein sequence evolution. The clade-specific models we generated may be useful tools for protein phylogenetics, and the structure of evolutionary model space that they revealed has implications for phylogenomic inference across the tree of life.
Enzymatic Glyco-Modification of Synthetic Membrane Systems
Authors: Jabeguero Dylan , Siukstaite Lina , Wang Chunyue , Mitrovic Anna , Pérez Serge , Makshakova Olga , Richter Ralf P. , Römer Winfried , Breton Christelle
Published: February 2023

The present report assesses the capability of a soluble glycosyltransferase to modify glycolipids organized in two synthetic membrane systems that are attractive models to mimic cell membranes: giant unilamellar vesicles (GUVs) and supported lipid bilayers (SLBs). The objective was to synthesize the Gb3 antigen (Galα1,4Galβ1,4Glcβ-Cer), a cancer biomarker, at the surface of these membrane models. A soluble form of LgtC that adds a galactose residue from UDP-Gal to lactose-containing acceptors was selected. Although less efficient than with lactose, the ability of LgtC to utilize lactosyl–ceramide as an acceptor was demonstrated on GUVs and SLBs. The reaction was monitored using the B-subunit of Shiga toxin as Gb3-binding lectin. Quartz crystal microbalance with dissipation analysis showed that transient binding of LgtC at the membrane surface was sufficient for a productive conversion of LacCer to Gb3. Molecular dynamics simulations provided structural elements to help rationalize experimental data.
Time-Course Transcriptome Analysis of the Lungs of Mice Challenged with Aerosols of Methicillin-Resistant Staphylococcus aureus USA300 Clone Reveals Inflammatory Balance
Authors: Zhao Yue , Zhai Lina , Qin Tongtong , Hu Lingfei , Wang Jiazhen , Zhang Zhijun , Sui Chengyu , Zhang Lili , Zhou Dongsheng , Lv Meng , Yang Wenhui
Published: February 2023

USA300, a dominant clone of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA), is circulating globally and can cause necrotizing pneumonia with high morbidity and mortality. To further reveal the host anti-MRSA infection immune response, we established a mouse model of acute primary MRSA pneumonia challenged with aerosols of the USA300 clone. A time-course transcriptome analysis of the lungs collected at 0, 12, 24, 48 and 96 h post-infection (hpi) was conducted using RNA sequencing (RNA-seq) and multiple bioinformatic analysis methods. The change trend of histopathology and five innate immune cell (neutrophils, mononuclear cells, eosinophils, macrophages, DC cells) proportions in the lungs after infection was also examined. We observed a distinct acute pulmonary recovery process. A rapid initiation period of inflammation was present at 12 hpi, during which the IL-17 pathway dominantly mediated inflammation and immune defense. The main stages of host inflammatory response occurred at 24 and 48 hpi, and the regulation of interferon activation and macrophage polarization played an important role in the control of inflammatory balance at this stage. At 96 hpi, cellular proliferation processes associated with host repair were observed, as well as adaptive immunity and complement system responses involving C1q molecules. More importantly, the data provide new insight into and identify potential functional genes involved in the checks and balances occurring between host anti-inflammatory and proinflammatory responses. To the best of our knowledge, this is the first study to investigate transcriptional responses throughout the inflammatory recovery process in the lungs after MRSA infection. Our study uncovers valuable research targets for key regulatory mechanisms underlying the pathogenesis of MRSA lung infections, which may help to develop novel treatment strategies for MRSA pneumonia.
In Vivo Acute Toxicity Studies of Novel Anti-Melanoma Compounds Downregulators of hnRNPH1/H2
Authors: Velayutham Sadeeshkumar , Seal Trisha , Danthurthy Samaya , Zaias Julia , Smalley Keiran S. M. , Minond Dmitriy
Published: February 2023

Despite the recent advances in melanoma therapy, the need for new targets and novel approaches to therapy is urgent. We previously reported melanoma actives that work via binding and downregulating spliceosomal proteins hnRNPH1 and H2. Given the lack of knowledge about the side effects of using spliceosomal binders in humans, an acute toxicity study was conducted to evaluate these compounds in mice. Male and female mice were treated with compounds 2155-14 and 2155-18 at 50 mg/kg/day via subcutaneous injections, and the clinical signs of distress were monitored for 21 days and compared with control mice. Additionally, the effect of the leads on blood chemistry, blood cell counts, and organs was evaluated. No significant changes were observed in the body weight, blood cell count, blood chemistry, or organs of the mice following the compound treatment. The results show that our compounds, 2155-14 and 2155-18, are not toxic for the study period of three weeks.
Reduced Glutathione-Modified Electrode for the Detection of Hydroxyl Free Radicals
Authors: Ghaedamini Hamidreza , Duanghathaipornsuk Surachet , Onusko Patrick , Binsheheween Abdullah M. , Kim Dong-Shik
Published: February 2023

Hydroxyl radicals (•OH) are known as essential chemicals for cells to maintain their normal functions and defensive responses. However, a high concentration of •OH may cause oxidative stress-related diseases, such as cancer, inflammation, and cardiovascular disorders. Therefore, •OH can be used as a biomarker to detect the onset of these disorders at an early stage. Reduced glutathione (GSH), a well-known tripeptide for its antioxidant capacity against reactive oxygen species (ROS), was immobilized on a screen-printed carbon electrode (SPCE) to develop a real-time detection sensor with a high selectivity towards •OH. The signals produced by the interaction of the GSH-modified sensor and •OH were characterized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The CV curve of the GSH-modified sensor in the Fenton reagent exhibited a pair of well-defined peaks, demonstrating the redox reaction of the electrochemical sensor and •OH. The sensor showed a linear relationship between the redox response and the concentration of •OH with a limit of detection (LOD) of 49 µM. Furthermore, using EIS studies, the proposed sensor demonstrated the capability of differentiating •OH from hydrogen peroxide (H2O2), a similar oxidizing chemical. After being immersed in the Fenton solution for 1 hr, redox peaks in the CV curve of the GSH-modified electrode disappeared, revealing that the immobilized GSH on the electrode was oxidized and turned to glutathione disulfide (GSSG). However, it was demonstrated that the oxidized GSH surface could be reversed back to the reduced state by reacting with a solution of glutathione reductase (GR) and nicotinamide adenine dinucleotide phosphate (NADPH), and possibly reused for •OH detection.
An Insight into the Suitability of Clayey Raw Materials: The Ceramic Provinces of the Northern Peloponnese and South Epirus, Greece
Authors: Xanthopoulou Vayia , Iliopoulos Ioannis
Published: February 2023

Corinth, Achaea in the northern Peloponnese, and Nicopolis in Epirus (Greece) have a strong archaeological record of ceramic production, from the Early Helladic to Late Roman periods. Important archaeological sites, such as Helike, Aigeira, the settlement of Derveni, and the cities of Patras and Nicopolis, offer data and findings of exceptional quality. One of the main pillars for the development of the ceramic production in these areas was the availability of local natural resources, among which are the clayey sedimentary deposits that outcrop in the area. This study investigates the physical-technological properties (plasticity and granulometry), the mineralogical composition of clayey raw materials, in order to test their suitability for ceramic production across the entire geographic area. Our aim was to differentiate the three main “ceramic” provinces, based upon the above-mentioned properties. New data from western Achaea and south Epirus are presented and compared with previous research conducted in Corinthia and eastern Achaea. The results obtained showed that the clays sampled from Corinthia and eastern Achaea are carbonatic materials of poor plasticity, whereas those from western Achaea and Epirus are either Ca-rich or Ca-poor materials, with the majority exhibiting a better plasticity. The results from this study are expected to constitute an important archaeometric tool for tackling archaeological issues by means of provenance and ceramic technology within the region and in the surrounding areas.
CRITIC-TOPSIS-Based Evaluation of Smart Community Safety: A Case Study of Shenzhen, China
Authors: Wang Chenyang , Wang Linxiu , Gu Tiantian , Yin Jiyao , Hao Enyang
Published: February 2023

As a micro-unit of the smart city, smart communities have transformed residents’ lives into a world that connects physical objects. Simultaneously, though, they have brought community safety problems. Most studies of the smart community have only focused on technical aspects, and little attention has been paid to community safety. Thus, this paper aims to develop an evaluation system for smart community safety, which will further promote community safety development. On the basis of identifying evaluation indicators, an evaluation framework was built to assess the level of smart community safety by a comprehensive CRITIC-TOPSIS method. Five smart communities in Shenzhen city were selected as cases to validate the feasibility of the evaluation framework. There was an indication that the indicator with the highest weight was the ‘building monitoring’, and the indicator with the lowest weight was the ‘emergency shelter guidelines’. In addition, the Yucun community showed the highest safety level among these five smart communities. Some suggestions for enhancing the safety level of the smart community are proposed, such as strengthening the training of community safety management talents, establishing good emergency protective measures, and encouraging residents to participate in the development of community safety. This research not only provides an innovative community safety assessment method; it also enriches the knowledge of smart community safety.
Amplification in Mechanical Properties of a Lead Rubber Bearing for Various Exposure Times to Low Temperature
Authors: Yasar Cansu , Karuk Volkan , Kaplan Onur , Cavdar Esengul , Ozdemir Gokhan
Published: February 2023

In this paper, new formulations to predict the change in mechanical properties, namely, post-yield stiffness and characteristic strength of lead rubber bearings (LRBs) at low ambient temperatures, are proposed based on test results. Proposed formulations consider not only the effect of low temperature but also the effect of exposure time to low temperature. Accordingly, a full-scale LRB was tested dynamically after being conditioned at temperatures of −20, −10, 0, and 20 °C for 3, 6, and 24 h. During the displacement-controlled cyclic tests, various levels of shear strain were applied to the isolator with loading frequencies of 0.1 Hz and 0.5 Hz. Then, force-displacement curves of LRB were recorded, and the corresponding amplifications in its hysteretic properties were noted. The accuracy of existing equations to estimate the amount of amplification in mechanical properties was evaluated through the experimental results. It was found that the existing formulas do not represent the effect of exposure time on LRB characteristics at low temperatures. On the other hand, the proposed equations result in highly accurate estimations of post-yield stiffness and characteristic strength of LRB at low temperatures for different exposure times.
Development of Side Silicone Mold for Reducing Shape Error of Free-Form Concrete Panel
Authors: Jeong Kyeongtae , Lee Moonse , Oh Jisu , Lee Donghoon
Published: February 2023

Errors that occur on the side shape of Free-form Concrete Panels (FCP) can cause errors in the FCP construction stage. Therefore, the error generated in FCPs must be reduced. Accordingly, side mold development and research are being carried out. However, in the case of studies using side silicone molds, there was no detailed information about the specifications and application methods of molds, and there was no support between molds. When producing FCPs with the method stated above, there is a high possibility for the mold to be pushed due to the side pressure of concrete, which can cause errors on the side shape of FCPs. Therefore, two new types of side silicone molds were developed in this study. In order to verify the performance difference between the newly developed mold and existing molds, FCP production tests and error analysis were performed. In result, the developed mold decreased the average error difference of the FCP side by 3–5 times compared to the existing mold. In addition, the significance of the average error difference with the produced FCP was verified, and results showed that the difference of the average error was significant at a 95% confidence level.
Mesenchymal Stromal Cell-Based Therapy
Authors: Mönch Dina , Reinders Marlies E. J. , Hoogduijn Martin J. , Dahlke Marc-Hendrik
Published: February 2023

The use of mesenchymal stromal cells (MSCs) for clinical application is intensively investigated for a variety of areas, such as bone repair, haematological and autoimmune diseases, and solid organ transplantation [...]
The Chloroplast Envelope of Angiosperms Contains a Peptidoglycan Layer
Authors: Tran Xuan , Keskin Erva , Winkler Paul , Braun Marvin , Kolukisaoglu Üner
Published: February 2023

Plastids in plants are assumed to have evolved from cyanobacteria as they have maintained several bacterial features. Recently, peptidoglycans, as bacterial cell wall components, have been shown to exist in the envelopes of moss chloroplasts. Phylogenomic comparisons of bacterial and plant genomes have raised the question of whether such structures are also part of chloroplasts in angiosperms. To address this question, we visualized canonical amino acids of peptidoglycan around chloroplasts of Arabidopsis and Nicotiana via click chemistry and fluorescence microscopy. Additional detection by different peptidoglycan-binding proteins from bacteria and animals supported this observation. Further Arabidopsis experiments with D-cycloserine and AtMurE knock-out lines, both affecting putative peptidoglycan biosynthesis, revealed a central role of this pathway in plastid genesis and division. Taken together, these results indicate that peptidoglycans are integral parts of plastids in the whole plant lineage. Elucidating their biosynthesis and further roles in the function of these organelles is yet to be achieved.
Interneuronal In Vivo Transfer of Synaptic Proteins
Authors: Klinkenberg Michael , Helwig Michael , Pinto-Costa Rita , Rollar Angela , Rusconi Raffaella , Di Monte Donato A. , Ulusoy Ayse
Published: February 2023

Neuron-to-neuron transfer of pathogenic α-synuclein species is a mechanism of likely relevance to Parkinson’s disease development. Experimentally, interneuronal α-synuclein spreading from the low brainstem toward higher brain regions can be reproduced by the administration of AAV vectors encoding for α-synuclein into the mouse vagus nerve. The aim of this study was to determine whether α-synuclein’s spreading ability is shared by other proteins. Given α-synuclein synaptic localization, experiments involved intravagal injections of AAVs encoding for other synaptic proteins, β-synuclein, VAMP2, or SNAP25. Administration of AAV-VAMP2 or AAV-SNAP25 caused robust transduction of either of the proteins in the dorsal medulla oblongata but was not followed by interneuronal VAMP2 or SNAP25 transfer and caudo-rostral spreading. In contrast, AAV-mediated β-synuclein overexpression triggered its spreading to more frontal brain regions. The aggregate formation was investigated as a potential mechanism involved in protein spreading, and consistent with this hypothesis, results showed that overexpression of β-synuclein, but not VAMP2 or SNAP25, in the dorsal medulla oblongata was associated with pronounced protein aggregation. Data indicate that interneuronal protein transfer is not a mere consequence of increased expression or synaptic localization. It is rather promoted by structural/functional characteristics of synuclein proteins that likely include their tendency to form aggregate species.
Molecular Composition and Biological Activity of a Novel Acetonitrile–Water Extract of Lens Culinaris Medik in Murine Native Cells and Cell Lines Exposed to Different Chemotherapeutics Using Mass Spectrometry
Authors: Di Turi Annamaria , Antonacci Marina , Dibenedetto Jacopo Raffaele , Maqoud Fatima , Leonetti Francesco , Centoducati Gerardo , Colonna Nicola , Tricarico Domenico
Published: February 2023

We evaluated the effects of a new extract (70% acetonitrile, 2E0217022196DIPFARMTDA) of Lens culinaris Medik (Terre di Altamura SRL, Altamura BA) to prevent cytotoxic damage from cisplatin, staurosporine, irinotecan, doxorubicin, and the glucocorticoid dexamethasone. The acetonitrile–water extract (range 0.1–5 mg/mL) was obtained by extracting 10 g of lentil flour with 50 milliliters of the acetonitrile–water extraction mixture in a 70:30 ratio, first for 3 h and then overnight in a shaker at room temperature. The next day, the extract was filtered and passed through a Rotavapor to obtain only the aqueous component and eliminate that with acetonitrile, and then freeze-dried to finally have the powdered extract. In vitro experiments showed that the extract prevented the cytotoxic damage induced by cisplatin, irinotecan, and doxorubicin on HEK293 and SHSY5Y cell lines after 24–96 h. In murine osteoblasts after 24–72 h of incubation time, the extract was cytoprotective against all chemicals. The extract was effective against dexamethasone, leading to synergic cell proliferation in all cell types. In bone marrow cells, the extract is cytoprotective after 72 h against doxorubicin, staurosporine, and dexamethasone. Instead, on muscle fibers, the extract has a synergic effect with chemotherapeutics, increasing cytotoxicity induced by doxorubicin and staurosporine. LC-MS attested to the existence of several phenolic structures in the extract. The most abundant families of compounds were flavonoids (25.7%) and mellitic acid (18%). Thus, the development of this extract could be implemented in the area of research related to the chemoprevention of damage to renal, neuronal, bone marrow cells, and osteoblasts by chemotherapeutics; moreover, it could be used as a reinforcer of cytotoxic action of chemotherapeutics on muscle fibers.
Effect of Surface Modification of PEEK Artificial Phalanx by 3D Printing on its Biological Activity
Authors: Shi Yun , Deng Ting , Peng Yu , Qin Zugan , Ramalingam Murugan , Pan Yang , Chen Cheng , Zhao Feng , Cheng Lijia , Liu Juan
Published: February 2023

Objective: Polyetheretherketone (PEEK) is widely used as an orthopedic implant material owing to its good biocompatibility and mechanical strength; however, PEEK implants are biologically inert, resulting in suboptimal cellular responses after implantation. The aim of this study was to enhance the biological activity of PEEK through sulfonation treatment. Methods: In this study, distal phalangeal implants of PEEK were customized by fused deposition modeling (FDM) printing technology and soaked in concentrated sulfuric acid at different times to obtain sulfonated PEEK (SPEEK). The groups were divided into five groups according to the sulfonation time as follows: 0 min (control group), 1 min (group SPEEK1), 2 min (group SPEEK2), 4 min (group SPEEK4), and 8 min (group SPEEK8). Then the physicochemical characteristics of implants were determined by SEM, XRD, EDS, etc. The implants were co-cultured with stem cells from human exfoliated deciduous teeth (SHED), and then the cell proliferation, adhesion, alkaline phosphatase (ALP) activity, and alizarin red staining were performed to detect the biological activity, biocompatibility, and osteogenic activity of the SPEEK implants. Results: The sulfonation time range of 1 to 8 min could promote the formation of micropores on the surface of PEEK implants, while slightly affecting the composition and compression performance of the implants. Compared with the control group, the hydrophilicity of PEEK materials was not improved after sulfonation treatment. Tests for adhesion and proliferation of SHED indicated that SPEEK2 showed superior biocompatibility. Furthermore, ALP activity and semi-quantitative analysis of Alizarin red staining showed that the osteogenic activity of SPEEK2 phalanges exhibited significantly stronger osteogenic activity than the other groups. Conclusions: The method presented here provides a promising approach to improve the surface bioactivity of PEEK implants prepared by FDM, providing a shred of primary evidence to support the application of SPEEK in orthopedics.
Investigating the Effects of Geometrical Parameters of Re-Entrant Cells of Aluminum 7075-T651 Auxetic Structures on Fatigue Life
Authors: Ghiasvand Amir , Khanigi Alireza Fayazi , Guerrero John William Grimaldo , Derazkola Hamed Aghajani , Tomków Jacek , Janeczek Anna , Wolski Adrian
Published: February 2023

In this study, the effects of two geometrical parameters of the re-entrant auxetic cells, namely, internal cell angle (θ) and H/L ratio in which H is the cell height, and L is the cell length, have been studied on the variations of Poisson’s ratio and fatigue life of Aluminum 7075-T6 auxetic structures. Five different values of both the H/L ratio and angle θ were selected. Numerical simulations and fatigue life predictions have been conducted through the use of ABAQUS (version 2022) and MSC Fatigue (version 11.0) software. Results revealed that increases in both the H/L ratio and angle θ improved the average value of Poisson’s ratio. Increasing the H/L ratio from 1 to 1.4 and θ from 50° to 70° increased the values of Poisson’s ratio, respectively, 7.7% and 80%. In all angles, increasing the H/L values decreased the fatigue life of the structures significantly. Furthermore, in all H/L values, an increment in θ caused a reduction in fatigue life. The effects of H/L and θ parameters on fatigue life were dominant in the low cycle fatigue regime. Results also showed that the H/L ratio parameter had greater influence as compared to the θ angle, and the structures with higher auxeticity experienced higher fatigue resistance. It was found that the auxetic property of the structure has a direct relationship with the fatigue resistance of the structure. In all samples, structures with greater auxetic property had higher fatigue resistance.
Enhanced Multimodal Effect of Chemotherapy, Hyperthermia and Magneto-Mechanic Actuation of Silver-Coated Magnetite on Cancer Cells
Authors: Herea Dumitru Daniel , Zară-Dănceanu Camelia-Mihaela , Lăbușcă Luminița , Minuti Anca-Emanuela , Stavilă Cristina , Ababei Gabriel , Tibu Mihai , Grigoraș Marian , Lostun Mihaela , Stoian George , Dragoș-Pînzaru Oana-Georgiana , Buema Gabriela , Chiriac Horia , Lupu Nicoleta
Published: February 2023

Currently, various methods based on magnetic nanoparticles are being considered for the treatment of cancer. Among these, magnetic hyperthermia and magneto-mechanical actuation are the most tested physical methods that have shown promising results when applied both separately and in combination. However, combining them with specific drugs can further improve antitumor efficiency. In this study, we performed a systematic analysis to determine the best combination of hyperthermia, magneto-mechanical actuation of silver-coated magnetite nanoparticles (MNP@Ag) and chemotherapy (mitoxantrone) capable of destroying tumor cells in vitro while maintaining normal cells in their state of increased viability. The results showed that of the nine treatment configurations, the only one that satisfied the safety condition for normal cells (fibroblasts) and the highly cytotoxic condition for tumor cells (HeLa) was the combination of all three triggers. This combination led to the decrease in HeLa viability to about 32%, while the decrease in fibroblast viability reached 80%. It was observed that the cytotoxic effect was not a sum of the separate effects of each trigger involved, but the result of a nonlinear conjugation of the triggers in a dynamic regime imposed by the magneto-mechanical actuation of the nanoparticles. We conclude that by using such a treatment approach, the need for chemotherapeutic drugs can be substantially reduced while maintaining their therapeutic performance.
Green Biosynthesis of Silver Nanoparticles Using Vaccinium oxycoccos (Cranberry) Extract and Evaluation of Their Biomedical Potential
Authors: Rizwana Humaira , Khan Mujeeb , Aldehaish Horiah A. , Adil Syed Farooq , Shaik Mohammed Rafi , Assal Mohamed E. , Hatshan Mohammad Rafe , Siddiqui Mohammed Rafiq H.
Published: February 2023

Eco-friendly preparation of metallic nanoparticles (NPs) is a greatly evolving field of scientific research. These types of NPs have gained substantial recognition from scientists, including chemists, chemical biologists and technologists, who have successfully exploited them for the fabrication of a variety of advanced nanodevices. Herein, silver (Ag) NPs were synthesized by a green approach using the aqueous extract of Vaccinium oxycoccos (cranberry), which not only reduced the silver ions but also stabilized the surface of the resultant Ag NPs. The formation of Ag NPs is confirmed by different analytical techniques, including powder X-ray diffraction, UV analysis, scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), transmission electron microscopy (TEM) and Fourier-transform infrared spectroscopy (FT-IR). The UV analysis of the aqueous solution of the reaction mixture demonstrated an absorption band at ~450 nm, which is the typical peak of Ag NPs, leading to the confirmation of product formation. While the XRD confirmed the crystallinity of the sample and the formation of a face-centered cubic (fcc) structure, on the other hand, TEM revealed the presence of spherical NPs with an approximate size range between 5–30 nm. Furthermore, the as-obtained Ag NPs were subjected to thorough investigations to explore the biomedical potential of the sample. In this case, the Ag NPs demonstrated considerable antioxidant and antifungal properties towards various pathogens. In addition, Ag NPs also showed substantial inhibition of spore germination.
Photon-Counting Computed Tomography (PCCT): Technical Background and Cardio-Vascular Applications
Authors: Meloni Antonella , Frijia Francesca , Panetta Daniele , Degiorgi Giulia , De Gori Carmelo , Maffei Erica , Clemente Alberto , Positano Vincenzo , Cademartiri Filippo
Published: February 2023

Photon-counting computed tomography (PCCT) is a new advanced imaging technique that is going to transform the standard clinical use of computed tomography (CT) imaging. Photon-counting detectors resolve the number of photons and the incident X-ray energy spectrum into multiple energy bins. Compared with conventional CT technology, PCCT offers the advantages of improved spatial and contrast resolution, reduction of image noise and artifacts, reduced radiation exposure, and multi-energy/multi-parametric imaging based on the atomic properties of tissues, with the consequent possibility to use different contrast agents and improve quantitative imaging. This narrative review first briefly describes the technical principles and the benefits of photon-counting CT and then provides a synthetic outline of the current literature on its use for vascular imaging.
The Role of Multimodality Imaging Approach in Acute Aortic Syndromes: Diagnosis, Complications, and Clinical Management
Authors: Perone Francesco , Guglielmo Marco , Coceani Michele , La Mura Lucia , Dentamaro Ilaria , Sabatino Jolanda , Gimelli Alessia
Published: February 2023

Acute aortic syndromes are life-threatening conditions with high morbidity and mortality. The principal pathological feature is acute wall damage with possible evolution towards aortic rupture. Accurate and timely diagnosis is mandatory to avoid catastrophic consequences. Indeed, misdiagnosis with other conditions mimicking acute aortic syndromes is associated with premature death. In this view, cardiovascular imaging is necessary for the correct diagnosis and management. Echocardiography, computed tomography, magnetic resonance imaging, and aortography allow for diagnosis, guarantee immediate treatment, and detect associated complications. Multimodality imaging is essential in the diagnostic work-up to confirm or rule out acute aortic syndromes. The aim of this review is to highlight the contemporary evidence on the role of single cardiovascular imaging techniques and multimodality imaging in the diagnosis and management of acute aortic syndromes.