bluuebunny/crossref_metadata_2025 huggingface dataset:
Olena Pchilka about "The first Wreath"
Authors: Скупейко Лукаш
Published: October 2024

Альманах «Перший вінок» вийшов 1887 р. у Львові. Ініціаторкою видання була Наталія Кобринська, на той час уже відома активістка й організаторка жіночого культурного руху в Галичині. Незвичність і навіть незвичайність видання полягала насамперед у тому, що в альманасі брали участь лише жінки-письменниці.
Regeneration patterns of multicultural values in the Muneng Pilgrimage Tradition in Temanggung, Indonesia
Authors: Anggraeni Laela Dian , Supriadi Didik
Published: October 2024

This research answers regeneration patterns and multicultural values ​​in the Muneng pilgrimage tradition. Introducing traditions to the younger generation is very important. Society cannot avoid generations because these habits are inherent and have their own meaning for a society. The results of this research show that the regeneration carried out in the Muneng pilgrimage tradition culture needs to include regeneration of cultural presenters, cultural implementers and cultural viewers. The methods used are the value of respect, the value of tolerance, the value of respect, the value of cooperation. This research aims to describe the multicultural values ​​of the Muneng pilgrimage tradition. The method used is the historical method. This historical method has steps that include processional heuristics and offerings, criticism in the form of authentic tests rather than external and internal, interpretation of historical analysis, and historiography. The results nclude 1) Regeneration is carried out with the involvement of all ages, 2) The Muneng pilgrimage becomes a motif of belief and becomes a means of preserving culture in Muneng, 3) The values ​​in culture result in harmonization in life.
Implementasi Media Komunikasi Bagi Mahasiswa Perekam Medis Sebagai Penyedia Informasi Kesehatan
Authors: Hermawan Dedy , Apriyanti Firda , Hatna Hatna
Published: October 2024

This research examines the utilization of communication platforms by medical record students to disseminate health information effectively. In today's information-driven era, especially within healthcare, the role of efficient communication tools is paramount. The study investigates how various media, including social media, electronic health records, and traditional channels, enhance the ability of medical record students to distribute accurate health information. Qualitative methods are employed to analyze the effectiveness of these communication channels in educating the public about health issues. The findings suggest that utilizing these platforms not only enhances students' proficiency in managing health information but also contributes to improving public health literacy. The study underscores the significance of integrating modern communication technologies into health education curricula. This integration is crucial for equipping future healthcare professionals with the skills needed to serve as trustworthy sources of health information.
Pengaruh Proporsi Ekstrak Daun Kersen (Muntingia calabura Linn) dan Ekstrak Daun Sirsak (Annona muricata Linn) terhadap Aktivitas Antioksidan dan Daya Terima Yoghurt
Authors: Husna Zulhidayatil , Syainah Ermina
Published: October 2024

Background: Indonesia ranks first with the most cancer cases in Southeast Asia. Degenerative diseases such as cancer occur due to the induction of free radicals, substances that can counteract and prevent damage caused by free radicals are called antioxidants. Objectives:To determine the effect of the proportions of kersen leaf extract and soursop leaf extract on the antioxidant activity and acceptability of yogurt. Methods: This research is experimental with a true experimental design (posttest with control group design). This research consists of 2 stages. The first stage, namely the introduction, aimed to determine the proportion of kersen leaf extract and soursop leaf extract that were most widely accepted/preferred by panelists from the 5 treatments given (P1 = 100 ml:0 ml, P2 = 75ml:25 ml, P3 = 50ml:50 ml, P4 = 25 ml:75 ml, P5 = 0ml:100 ml). The second stage or main research aims to determine the chemical quality (antioxidant activity) in the selected treatment. Results: The results of the organoleptic test showed that all hedonic categories, namely aroma, color and taste except for texture, had significant differences at p <0.05. The best proportion was obtained in the P2 treatment, namely the proportion of 75ml DK : 25ml DS. The P2 treatment increased the antioxidant activity of yogurt, which was 52.33ppm compared to the control yogurt, which had an antioxidant activity of 73.78ppm. Conclusions: There is an effect of the proportion of kersen leaf extract and soursop leaf extract on the antioxidant activity and acceptability of yogurt except texture.
Faktor yang Mempengaruhi Preferensi Makanan pada Mahasiswa Fakultas Kesehatan Masyarakat, Universitas Andalas
Authors: Nur Nadia Chalida , Lova Luvia Milda , Symond Denas
Published: October 2024

A person's food preferences can be influenced by various factors, including individual characteristics, food characteristics, and environmental characteristics. The study conducted aimed to determine the relationship between several factors, such as money allowance, food expenditure, parents' occupation, nutrition knowledge, food availability, and sensory appeal, and the food preferences of students in the Public Health Faculty at Andalas University.  This quantitative study used a cross-sectional design. The research was conducted from June to July 2023. The sample consisted of 100 students who were selected using proportional stratified random sampling technique. Data were collected through questionnaires on money allowance, food expenditure, nutritional knowledge, food availability, sensory appeal, and food preferences. The chi-square test was used for data analysis.  The study found that money allowance (p-value=0.030), food expenditure (p-value=0.001), and nutrition knowledge (p-value=0.009) were significantly related to students' food preferences. The father’s occupation (p=1,000), mother’s occupation (p=0.549), food availability (p=0,643), and sensory appeal (p=0.643) had no significant relationship with students' food preferences. It is recommended that students consider rational factors when making decisions about their food preferences.
 
Keywords: food expenditure, food preferences, pocket money, sensory appeal.
Meningkatkan Motivasi Belajar Unsur Siswa Kelas VIII SMPN 2 Telukjambe Barat melalui Model Pembelajaran STAD
Authors: Jauharo Agustin Farras , Mahmudatussaadah Ai
Published: October 2024

Mata pelajaran Ilmu Pengetahuan Alam (IPA) memiliki banyak aspek yang sulit dipahami siswa sehingga membuat siswa sulit termotivasi mempelajari IPA. Padahal motivasi sangatlah penting dalam kegiatan belajar karena belajar tanpa atau kurang motivasi tidak akan berhasil dengan maksimal. Model pembelajaran merupakan salah satu faktor yang secara signifikan memengaruhi motivasi belajar. Salah satu model pembelajaran yang telah banyak diaplikasikan untuk memperbaiki kualitas pembelajaran yaitu model pembelajaran kooperatif tipe student teams achievement division (STAD). Penelitian ini bertujuan untuk memperbaiki kualitas pembelajaran IPA, tepatnya materi unsur dalam hal peningkatan motivasi belajar siswa dengan cara menerapkan model pembelajaran kooperatif tipe STAD di kelas VIII SMPN 2 Telukjambe Barat. Metodologi penelitian yang digunakan yaitu penelitian tindakan kelas dengan lembar evaluasi siswa, formulir Alat Penilaian Kinerja Guru, dan lembar observasi sebagai instrumen penelitian. Hasil analisis data mengungkapkan bahwa terjadi peningkatan nilai tes rata-rata siswa dari 30,86 menjadi 49,43. Nilai modul ajar dan nilai praktik perbaikan pembelajaran juga mengalami peningkatan. Pada lembar observasi juga terdapat penambahan kriteria yang ditandai ada (muncul). Berdasarkan hal tersebut, dapat disimpulkan bahwa model pembelajaran kooperatif tipe student teams achievement division (STAD) dapat meningkatkan motivasi belajar IPA materi Unsur siswa kelas VIII G SMPN 2 Telukjambe Barat.
Computational identification and characterization of chitinase 1 and chitinase 2 from neotropical isolates of Beauveria bassiana
Authors: Segura-Vega Juan , González-Herrera Allan , Molina-Bravo Ramón , Solano-González Stefany
Published: October 2024

Background

The fungus Beauveria bassiana is widely used for agronomical applications, mainly in biological control. B. bassiana uses chitinase enzymes to degrade chitin, a major chemical component found in insect exoskeletons and fungal cell walls. However, until recently, genomic information on neotropical isolates, as well as their metabolic and biotechnological potential, has been limited.

Methods

Eight complete B. bassiana genomes of Neotropical origin and three references were studied to identify chitinase genes and its corresponding proteins, which were curated and characterized using manual curation and computational tools. We conducted a computational study to highlight functional differences and similarities for chitinase proteins in these Neotropical isolates.

Results

Eleven chitinase 1 genes were identified, categorized as chitinase 1.1 and chitinase 1.2. Five chitinase 2 genes were identified but presented a higher sequence conservation across all sequences. Interestingly, physicochemical parameters were more similar between chitinase 1.1 and chitinase 2 than between chitinase 1.1 and 1.2.

Conclusion

Chitinases 1 and 2 demonstrated variations, especially within chitinase 1, which presented a potential paralog. These differences were observed in their physical parameters. Additionally, CHIT2 completely lacks a signal peptide. This implies that CHIT1 might be associated with infection processes, while CHIT2 could be involved in morphogenesis and cellular growth. Therefore, our work highlights the importance of computational studies on local isolates, providing valuable resources for further experimental validation. Intrinsic changes within local species can significantly impact our understanding of complex pathogen-host interactions and offer practical applications, such as biological control.
The effect of titanium surface treatment by application of constant potential or current on the viability of pre-osteoblast cells: an in-vitro study
Authors: Cai Wenji , Wang Min , Hadad Amir Ei , Zhang Yuli , Tran Simon D. , Shurbaji Samar , Nasrallah Gheyath K. , Sanz Mariano , Omanovic Sasha , Tamimi Faleh
Published: October 2024

Objectives

The aim of this study was to investigate the impact of electrochemical treatment of a titanium surface employing constant current and potential on the viability of the tissue cells attached to the surface and determining the safety limits for this type of treatment.

Methods

Pre-osteoblast cells (pOB) were cultured and seeded onto titanium discs. The cell-seeded discs were then exposed to a range of contant direct electrical potentials (-6V–6V) or contant direct electrical currents (−12.5 mA, −25 mA, or −50 mA) using a three-electrode system connected to a potentiostat. Cell viability was assessed using live/dead assay and fluorescence microscopy.

Results

Exposure of cells to high negative potentials caused cell detachment, while exposure to positive ones led to cell death on the cpTi surfaces. However, cellular viability was preserved when the electrical potentials were kept between −3 and +3 V. Cells retained 80% viability when subjected to −12.5 mA currents with an initial pOB cell count of 5 × 104. However, when the initial cell count was elevated to 1 × 105, the cells demonstrated the ability to withstand an even greater current (−25 mA) while preserving their vitality at the same level.

Conclusion

Treatment of a titanium dental implant surface employing constant potential or current can harm cells surrounding dental implants. However, this damage can be minimized by keeping the potential within a safety limit.
Selective and fast oxidation of alcohol to aldehyde using novel catalytic deep eutectic solvent surfactants
Authors: Shokr Chalaki Bahareh , Azizi Najmedin , Mirjafary Zohreh , Saeidian Hamid
Published: October 2024

Deep eutectic solvent (DES) has been considered as a useful catalyst and reaction medium for various organic transformations. Herein, we report the catalytic application of novel deep eutectic solvent- based surfactant (DES surfactant) for the selective and fast oxidation of alcohols to aldehydes. The readily accessible DES surfactants (FeCl3/BHDC) was prepared using inexpensive ferric chloride (FeCl3) and benzyl hexadecyl dimethyl ammonium chloride in a simple manner. The synthesized FeCl3/BHDC was characterized using various techniques, including, FTIR spectroscopy, thermal gravimetric analysis (TGA), scanning electron microscopy (SEM), and energy- dispersive X-ray spectroscopy (EDS) to determine its structure. The catalytic activity of FeCl3/BHDC in the selective oxidation of various alcohols to corresponding aldehyde derivative was investigated. The results showed the reaction could be completed within very short reaction times ranging from 2 to 15 min, while achieving good to excellent yields. This protocol offers a facile strategy and excellent efficiency in selectively oxidizing various alcohol derivatives to their respective aldehydes and ketones, utilizing hydrogen peroxide in the presence of catalytic DES surfactant.
Levels of high-sensitive troponin T and mid-regional pro-adrenomedullin after COVID-19 vaccination in vulnerable groups: monitoring cardiovascular safety of COVID-19 vaccination
Authors: Pudasaini Samipa , Le Ngoc Han , Huscher Dörte , Holert Fabian , Hillus David , Tober-Lau Pinkus , Kurth Florian , Sander Leif Erik , Möckel Martin
Published: October 2024

Background

COVID-19 vaccines are well tolerated and effective but may have adverse effects on the cardiovascular system. Vaccine-associated myocardial injury was analysed by measuring high-sensitive troponin T (hsTnT); mid-regional pro-adrenomedullin (MR-proADM) levels were evaluated to assess endothelial dysfunction.

Methods

This was a prospective study with a vulnerable population of healthcare workers (HCWs) and elderly patients (>70 years) who were vaccinated with either one dose of ChAdOx1 nCov-19 adenoviral vector vaccine (AZ) followed by one dose of the BNT162b2 messenger RNA vaccine (BNT), or with two doses of BNT (12th of January - 30th of November 2021). HsTnT and MR-proADM were measured in blood samples at three visits (V1: 1st immediately before vaccination; V2, 3: 3–4 weeks after 1st and 2nd vaccination). HsTnT of HCWs was compared to a healthy reference population.

Results

N = 162 volunteers were included (V1 = 161; V2, V3 = 162 each). N = 74 (45.7%) received AZ/BNT and n = 88 (54.3%) received BNT/BNT [elderly: n = 20 (12.3%), HCWs: n = 68 (42.0%)]. Median hsTnT levels were 4 ng/L, 5 ng/L and 4 ng/L (V1-V3) for AZ/BNT and at 5 ng/L, 6 ng/L and 6 ng/L (V1-V3) for BNT/BNT. Compared to the reference population (n = 300), hsTnT was significantly higher at all visits for both vaccination groups (p < 0.01), without differences between the AZ/BNT and BNT/BNT cohort. Median MR-proADM values were 0.43 nmol/L, 0.45 nmol/L, 0.44 nmol/L (V1-V3) in the AZ/BNT cohort and 0.49 nmol/L, 0.44 nmol/L, 0.47 nmol/L for BNT/BNT, respectively. Change of median hsTnT and MR-proADM between visits did not show significant increases. One HCW experienced a permanent and three a transient hsTnT increase ≥14 ng/L.

Conclusion

No overall subtle, persistent cardiovascular involvement was observed after the 2nd COVID-19 vaccination. Elevated cardiovascular biomarkers in clinically asymptomatic individuals need further investigations.
Research progress of sea buckthorn (Hippophae rhamnoides L.) in prevention and treatment of cardiovascular disease
Authors: Chen Yumeng , He Weiwei , Cao Hanjing , Wang Zhenzhen , Liu Jiping , Wang Bin , Wang Chuan
Published: October 2024

Sea buckthorn (Hippophae rhamnoides L.) contains a variety of biologically active compounds, including flavonoids, terpenoids, polysaccharides, organic acids, volatile oils, and vitamins. It has been demonstrated to be effective in the treatment of cardiovascular disorders. In this paper, we evaluated the pharmacological effects of sea buckthorn in cardiovascular diseases through preclinical studies, and revealed the mechanism of action of the active components in sea buckthorn in cardiovascular diseases, including anti-inflammatory, lipid oxidation regulation, antioxidant, vascular function modulation, anti-platelet aggregation, autophagy, intestinal microorganism regulation, and cell apoptosis reduction. In clinical trials, sea buckthorn was proven to be effective in managing lipid metabolism, blood pressure, and blood glucose levels in patients. We also extensively reviewed the safety of sea buckthorn medicine and its toxicity to numerous organs. To summarize, sea buckthorn has a beneficial effect on cardiovascular disease and may give a novel strategy for clinical intervention and therapy. This paper summarizes the phytochemistry, pharmacology, clinical applications, safety, and toxicity of sea buckthorn in order to better understand the mechanism of action of the various bioactive components in sea buckthorn, investigate its medicinal potential, and provide more options for the treatment of cardiovascular diseases.
Improvement of the outcome of the saphenous vein graft when connected to the internal thoracic artery
Authors: Katsavrias Konstantinos , Prapas Sotirios , Calafiore Antonio M. , Taggart David , Angouras Dimitrios , Iliopoulos Dimitrios , Di Mauro Michele , Papandreopoulos Styliani , Zografos Panayiotis , Dougenis Dimitrios
Published: October 2024

Background

Since 2000, we have been grafting the right coronary artery system (RCAs) using the proximal portion of the right internal thoracic artery (RITA) as the inflow of the saphenous vein graft (SVG) to increase the number of patients undergoing beating heart complete myocardial revascularization.

Methods

From 2000 to 2022, 928 consecutive patients underwent SVG on the RCAs. In 546 patients (58.8%), the inflow was the RITA (I-graft group), and in 382 patients (41.2%), the inflow was the aorta (Ao-graft group). The inclusion criteria were age ≤75 years, ejection fraction >35%, only one SVG per patient, bilateral internal thoracic arteries as a Y-graft on the left system (three-vessel disease, n = 817, 88.0%) or left internal thoracic artery on the left anterior descending artery and RITA + SVG on the RCAs (two-vessel disease, n = 111, 12.0%). Propensity matching identified 306 patients per group. After a median follow-up of 8 (5–10) years, graft patency was assessed by coronary computed tomographic angiography in 132 patients (64 in the I-graft group and 68 in the Ao-graft group).

Results

Early results were similar in both groups. The I-graft group had higher 10-year survival and freedom from main adverse cardiac events (90.0 ± 2.0 vs. 80.6 ± 3.8, p = 0.0162, and 81.3 ± 2.7 vs. 64.7 ± 5.6, p = 0.0206, respectively). When RITA was the inflow, SVG had a higher estimated 10-year patency rate (82.8% ± 6.5 vs. 58.8% ± 7.4, p = 0.0026) and a smaller inner lumen diameter (2.7 ± 0.4 vs. 3.4 ± 0.6 mm, p < 0.0001).

Conclusion

When the inflow is the RITA, SVG grafted to the RCAs (I-graft) may result in a higher patency rate and better outcome than when the inflow is the ascending aorta (Ao-graft). The continuous supply of nitric oxide by RITA may be the cause of the higher patency rate of the I-graft, which can behave like an arterial conduit.
A method for calculating saturation in tight sandstone reservoirs based on the dual porosity model
Authors: Xin Yi , Duan Wei , Han Chuang , Bie Kang , Zhao Xinjian , Ai Yong , Zhao Peiqiang
Published: October 2024

Introduction

With the continuous development of exploration and development, tight sandstone reservoirs have become an essential field of oil and gas exploration. The tight sandstone reservoirs are characterized by complex lithology, poor pore structure, and strong heterogeneity, which bring great difficulties to formation evaluation by well logs. Especially, the accuracy of the Archie formula for calculating the water saturation of tight sandstone reservoirs containing fractures is not high, saturation evaluation faces greater challenges. Therefore, how to calculate the saturation of tight sandstone reservoirs more accurately is an urgent problem to be solved.

Methods

In this paper, the tight sandstone reservoirs of the Jurassic Ahe Formation in the Tarim Basin were taken as the research area. A saturation model that combines the effects of shale and fractures was proposed. Specifically, if the shale content is more than 20%, the Indonesian formula is used to calculate the saturation. If the shale content is less than 20%, the dual porosity model is adopted. Based on the rock resistivity and nuclear magnetic resonance (NMR) experiment results, the porosity and T2 logarithmic mean values are selected as influencing factors to calculate the key parameters of the dual porosity model.

Results and Discussion

The saturation of tight sandstone reservoirs in the Jurassic Ahe Formation of the Tarim Basin is calculated through the method proposed in this paper. The case study shows that the accuracy of the proposed method is higher than that of the Archie model. The method proposed in this paper demonstrates excellent adaptability in the quantitative evaluation of saturation for tight sandstone reservoirs.
The role of gas emissions (He, Rn, and CO2) from fault zones in understanding fault and seismic activity
Authors: Li Jiye , Liu Zhaofei , Chen Zhi , Gao Yan , Hao Yongmei , Gu Hongbiao
Published: October 2024

Active fault zones are critical pathways for the migration of deep fluids to the Earth’s surface, carrying gases such as He, Rn, and CO2 that provide evidence for the physical and chemical dynamics of the Earth’s interior. This review examines the geochemical characteristics of fault zone gases and their implications for understanding fault activity and seismic events. Fault zones with high activity levels exhibit significant gas release, and variations in soil and hot spring gas concentrations can serve as indicators of seismic activity. Changes in gas concentrations and isotopic ratios, particularly before and after earthquakes, reflect the dynamic interplay between deep-sourced and shallow-sourced fluids. Seismic-induced stress alterations enhance gas release along fault zones, leading to observable anomalies that can aid in earthquake monitoring and prediction. The study underscores the importance of isotope tracing in deciphering fluid sources, migration pathways, and the evolution of fault zones, providing valuable information for assessing tectonic activity and mitigating seismic risks.
How higher education teachers see their professional identity
Authors: Halal Orfali Carol , Arancibia Muñoz Maria Luisa , Riquelme Plaza Irma , Unda Valenzuela Rodrigo
Published: October 2024

An important dimension to understand the student learning process is the professional identity of teachers, which is conceptualized as a continuous process of construction and reconstruction. In this process, teachers define and assume their roles in a specific educational context that is characterized by tensions, adjustments, and transformations. This study focuses on understanding the professional identity of higher education teachers, a field characterized by constant evolution and demands. The personal characteristics of this identity are explored, including experiences and biographical representations, as well as its interrelationship with the institutional environment. Using a qualitative interpretive approach, six teachers recognized for their pedagogical innovation and educational commitment were interviewed. The results reveal outstanding motivation and responsibility in educational work, but also tensions between personal identity and the work context, reflected in the perception of low recognition and the importance of institutional leadership. The teachers show a critical and reflective view of their work, influenced by childhood experiences that impact their conceptions of educational justice and equity. To conclude, the common characteristics of professional identity in higher education highlight the need to integrate them into professional development. The article advocates for the design of more personalized training programs and the promotion of continuous learning among peers as key elements to strengthen this identity and improve pedagogical practice.
Comparative analysis of frequently used e-learning platforms
Authors: Jain Shaily , Prabha Chander , Nandan Durgesh , Bhosale Snehal
Published: October 2024

An online e-learning platform known as a learning management system (LMS) provides an interface between trainers, students, and administrators toward a common objective of learning. In the market, diverse LMSs with extensive functionalities are available to facilitate e-learning. In this article, we selected some popular LMSs majorly used by most schools and institutions for their teaching–learning process. We compared these LMSs in a discussion based on their technical specifications, technical features, administrative features, general features, and course management-related features. This comparative analysis could help users decide which LMS suits them best, according to their requirements. We also attempted to identify the limitations of these learning management systems so that users and organizations can determine which one is best suited to their needs.
Associations of gestational thyrotropin levels with disease progression among pregnant women with differentiated thyroid cancer: a retrospective cohort study
Authors: Li Xin , Fu Peng , Xiao Wu-Cai , Mei Fang , Zhang Fan , Zhang Shanghang , Chen Jing , Shan Rui , Sun Bang-Kai , Song Shi-Bing , Yuan Chun-Hui , Liu Zheng
Published: October 2024

Purpose

Pregnant women with a diagnosis of differentiated thyroid cancer (DTC) were potentially high-risk but largely ignored study population. We aimed to explore whether gestational thyrotropin levels were associated with progression of DTC.

Methods

We conducted a retrospective cohort study at Peking University Third Hospital in Beijing, China from January 2012 to December 2022. We included pregnant women with a pre-pregnancy DTC managed by active surveillance (under-surveillance DTC) or surgical treatment (after-surgery DTC). Dynamic changes of gestational thyrotropin levels across multiple time points were characterized by both statistical (average level, change instability, longitudinal trajectory) and clinical (thyroid dysfunction, thyrotropin suppression, and achievement of thyrotropin suppression target) indicators. Outcomes were clinician-validated progression of DTC, measured separately for patients under surveillance (tumor enlargement or lymph node metastasis) and those after surgery (≥ 3 mm growth in the size of existing metastatic foci, development of new lymph node metastases, ≥ 2 mm growth in the size of existing cancer foci in the contralateral thyroid, or biochemical progression).

Results

Among 43 and 118 patients with under-surveillance and after-surgery DTC, we observed no evidence of associations between any of the quantitative or clinical indicators of gestational thyrotropin levels and progression-free survival, after a median of 2.63 (IQR: 0.90-4.73) and 4.22 (2.53-6.02) year follow-up, respectively (all P values > 0.05).

Conclusions

Gestational thyrotropin levels appeared to play a minor role in the progression of under-surveillance or after-surgery DTC. Clinicians might focus on the risk of adverse pregnancy outcomes when optimizing thyrotropin levels for pregnant women with a diagnosis of DTC.
Uric acid is associated with increased risk of myocardial infarction: results from NHANES 2009-2018 and bidirectional two-sample Mendelian randomization analysis
Authors: Deng Ting , Liu Xiaoying
Published: October 2024

Aim

Although a growing number of studies have shown that elevated uric acid (UA) levels are associated with multiple cardiovascular risk factors and progression of coronary artery disease, the causal relationship between UA and the occurrence of myocardial infarction (MI) remains uncertain. The aim of this study was to investigate the relationship between UA and the risk of MI.

Methods

We screened 23,080 patients in the National Health and Nutrition Examination Survey (NHANES) database for 2009-2018 and explored the association between UA and MI risk using multivariate logistic regression model. In addition, a two-way two-sample Mendelian randomization (TSMR) analysis was performed to examine the causal relationship of UA on MI, and inverse variance-weighted (IVW) results were used as the primary outcome in this study. Sensitivity analysis and horizontal multiple validity test were also performed to verify the reliability of the results.

Results

After multivariable adjustment, individuals with the severe elevation of UA levels have a significantly increased risk of MI (OR=2.843, 95%CI: 1.296-6.237, P=0.010). In TSMR analysis, the IVW method demonstrated a significant association between UA and increased risk of MI (OR=1.333, 95%CI: 1.079-1.647, P=0.008). Results from the MR-Egger intercept test, Cochran’s Q test, and MR-PRESSO test all suggest the reliability of the IVW analysis. Reverse TSMR analysis did not indicate a causal relationship between genetic susceptibility to MI and UA levels (IVW: OR=1.001, 95%CI: 0.989-1.012, P=0.922).

Conclusion

Based on cross-sectional study and Mendelian randomization analysis, it has been demonstrated that UA is an independent risk factor for MI. Elevated levels of UA increase the risk of MI, particularly in cases of severe elevation.
Anti-atherosclerotic effect of incretin receptor agonists
Authors: Wang Xin , Yang Xin , Qi Xiaoyan , Fan Gang , Zhou Lingzhi , Peng Zhengliang , Yang Jing
Published: October 2024

Incretin receptor agonists (IRAs), primarily composed of glucagon-like peptide-1 receptor agonists (GLP-1RAs) and glucose-dependent insulinotropic polypeptide receptor agonists (GIPRAs), work by mimicking the actions of the endogenous incretin hormones in the body. GLP-1RAs have been approved for use as monotherapy and in combination with GIPRAs for the management of type 2 diabetes mellitus (T2DM). In addition to their role in glucose regulation, IRAs have demonstrated various benefits such as cardiovascular protection, obesity management, and regulation of bone turnover. Some studies have suggested that IRAs not only aid in glycemic control but also exhibit anti-atherosclerotic effects. These agents have been shown to modulate lipid abnormalities, reduce blood pressure, and preserve the structural and functional integrity of the endothelium. Furthermore, IRAs have the ability to mitigate inflammation by inhibiting macrophage activation and promoting M2 polarization. Research has also indicated that IRAs can decrease macrophage foam cell formation and prevent vascular smooth muscle cell (VSMC) phenotype switching, which are pivotal in atheromatous plaque formation and stability. This review offers a comprehensive overview of the protective effects of IRAs in atherosclerotic disease, with a focus on their impact on atherogenesis.
Analyzing research trends in glioblastoma metabolism: a bibliometric review
Authors: Dai Jiaxin , Song Siyun , Chen Pengyu , Huang Qixuan , Duan Hubin
Published: October 2024

Background

A bibliometric and visual analysis of articles related to glioblastoma metabolism was conducted to reveal the dynamics of scientific development and to assist researchers in gaining a global perspective when exploring hotspots and trends.

Methods

The Web of Science Core Collection (WoSCC) was employed to search, screen, and download articles about glioblastoma metabolism published between 2014 and 2024. The relevant literature was analyzed using CiteSpace, VOSviewer and Microsoft Excel.

Results

A total of 729 articles were included for bibliometric analysis between 2014 and 2024, and the number of articles published each year showed an overall increasing trend, except for a decrease in the number of articles published in 2018 compared to 2017. Collaboration network analysis showed that the United States, Germany and China are influential countries in this field, with a high number of articles published, citations and collaborations with other countries. The journal with the largest number of published articles is the International Journal of Molecular Sciences. Mischel PS is the most prolific author with 14 articles, and Guo DL received the most citations with 104 citations. Keyword analysis of the literature showed that the “Warburg effect” achieved the highest burst intensity, and “central nervous system”, “classification” and “fatty acids” showed stronger citation bursts in 2024, indicating that they are still popular topics so far.

Conclusion

This article elucidates the research trends and focal points in the field of glioblastoma metabolism, furnishes invaluable insights into the historical and contemporary status of this field, and offers guidance for future research. Further research into glioblastoma metabolism will undoubtedly yield new insights that will inform the diagnosis and treatment of this disease.
Age-specific changes in the serum proteome of female anadromous, hilsa Tenualosa ilisha: a comparative analysis across developmental stages
Authors: Chakraborty Hena , Chakraborty Hirak Jyoti , Das Basanta Kumar , Maity Joydev
Published: October 2024

Introduction

The proteome profile of the female Tenualosa ilisha (Hamilton, 1822), a species of great ecological and economic importance, across various age groups was investigated to comprehend the functional dynamics of the serum proteome for conservation and aquaculture, as well as sustain the population.

Methods

Advanced liquid chromatography-tandem mass spectrometry LC-MS/MS-based proteomic data were analysed and submitted to the ProteomeXchange Consortium via PRIDE (PRoteomics IDEntifications database). Bioinformatics analysis of serum proteome have been done and it showed different proteins associated with GO Gene Ontology () terms, and the genes associated with enriched KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways (such as phagosome, mTOR, Apelin signalling pathways, herpes simplex virus) implicated in immune responses.

Results

The expression levels of important immunological proteins, such as those involved in cellular defence and inflammatory responses, were significantly different age-dependently. In this study, we annotated 952, 494, 415, and 282 proteins in year classes IV, III, II, and I Hilsa, respectively, and analysed their Protein–Protein Interaction (PPI) networks based on their functional characteristics. From year classes I to IV, new proteins appeared and were more than three-fold. Notably, class I hilsa displayed a lower abundance of proteins than class IV hilsa.

Discussion

This is the first study, to the best of our knowledge, to report the analysis of the serum proteome of hilsa at different developmental stages, and the results can help improve the understanding of the mechanisms underlying the different changes in protein enrichment during migration in hilsa. This analysis also offers crucial insights into the immune system for hilsa conservation and management.
Numerical modeling of an offshore shellfish farm exposed to extreme wave conditions
Authors: Yang Hui , Li Yihong , Wang Jun , Ma Yingchao , Xu Zhijing
Published: October 2024

Shellfish cultivation is a sustainable method of providing human food and can help remove large amounts of CO2 from the atmosphere. Over the last two decades, longline-based structures have dominated farming systems. So far, the innovative technologies for open-ocean shellfish farming remain stagnant and need to be developed. As such, this paper preliminarily studies the operation and survivability abilities of an innovative shellfish farm under extreme wave conditions. To that end, an efficient numerical scheme with a robust implicit finite element method is established. First, the numerical modeling of a single module of the shellfish farm is conducted and the numerical results are verified against physical model tests. Then, the numerical modeling is implemented in a full-scale shellfish farm containing nine floating rafts with suspended lantern nets in a 3×3 configuration exposed to extreme wave conditions. Different angles of wave attack and shellfish rafts with and without lantern nets are fully considered, allowing an assessment of the operation and survivability abilities of the shellfish farm under extreme wave conditions in various situations. The results highlight that the angle of wave attack significantly affected the energy absorption of the mooring system. Moreover, non-linear instability such as subharmonics, which existed in the motion dynamics, can be manipulated to avoid resonant motions. This study provides insights into the evaluation of the safety design of a shellfish farm at both operational and survivability levels. The numerical method can also model other advanced offshore marine structures with multi-modules, such as floating bridges, airports, and even floating energy islands.
Exploring and comparing renal adverse effects between PARP inhibitors based on a real-world analysis of post-marketing surveillance data
Authors: Xu Qiuyu , Jiang Lin , Chen Gang , Ai Sanxi , Fan Xiaohong , Wang Gangan , Jia Chunyu , Wang Jiahui , Zheng Ke , Zhao Bin , Qin Yan , Li Xuemei
Published: October 2024

Objective

Poly (ADP-ribose) polymerase inhibitors (PARPis) are emerging targeted therapeutic agents in oncology, primarily indicated for ovarian and metastatic breast cancer. Acute kidney injury (AKI) has been observed in patients undergoing PARPi treatment, while there is still a lack of comprehensive comparisons of AKI associated with different PARPis. Our study aimed to extensively characterize the renal adverse effects (RAEs) of PARPi using real-world data.

Methods

Disproportionality analysis and Bayesian analysis were employed for data mining to identify suspected RAE cases after different PARPis use within the Food and Drug Administration’s Adverse Event Reporting System from January 2004 to September 2023. The time to onset, fatality, and hospitalization rates of PARPi-related RAEs were also investigated.

Results

We identified 1,696 PARPi-related RAEs, predominantly affecting patients over 85 (56.31%). Veliparib exhibited a more pronounced association with RAEs compared to others, as indicated by the highest reporting odds ratio (ROR = 29.20, 95% CI = 8.79–96.97), proportional reporting ratio (PRR = 19.80, χ2 = 72.62), and empirical Bayes geometric mean (EBGM = 19.80, the lower 90% one-sided CI = 7.25). The median time to RAEs onset was 15 (interquartile range: 6–55.75) days following the initiation of PARPi therapy. PARPi-related RAEs generally led to a 28.15% hospitalization rate and a 4.34% fatality rate.

Conclusion

Although the majority present with reversible creatinine elevation, PARPi-related RAEs merits broader attention, given its potential for clinical consequences. We should strive to early identify those individuals who may have irreversible kidney damage. The focus should be directed toward monitoring renal function in individuals receiving PARPi, especially in senile people and those with a predisposition to AKI.
Building on the successes of patient-focused drug development: a call for new policies to maintain momentum
Authors: Martin Allison , DiBiaso Victoria , Bozic Mladen , May Alexander T.
Published: October 2024

Since its commencement as part of the Food and Drug Administration’s (FDA) Prescription Drug User Fee Act (PDUFA) V in 2012, patient-focused drug development (PFDD) has become an integral part of the drug development paradigm. FDA encourages the development and use of Patient-Experience Data (PED) as it provides important information on the patients’ needs and perspectives and inform regulatory decision-making. While the FDA is required to fill out a table which includes a list of various types of Patient Experience Data (PED) and if such data was reviewed by FDA as part of a drug application, there is still a need to understand how FDA uses PED in its regulatory decision-making. This article examines whether new policies are needed to ensure the full potential of PFDD.
Safety and efficacy of plasmapheresis in treatment of acute fatty liver of pregnancy—a systematic review and meta-analysis
Authors: Siwatch Sujata , De Arka , Kaur Bandhanjot , Lamba Divjot Singh , Kaur Simarpreet , Singh Virendra , Periyasamy Aravind Gandhi
Published: October 2024

Introduction

Acute fatty liver of pregnancy (AFLP) is a fatal disease occurring in 3rd trimester. The safety and efficacy of plasmapheresis/plasma exchange (PP/PE) as an adjunctive treatment in patients of AFLP has been studied. We performed systematic review and meta-analysis to estimate the clinical parameters that included mortality rates and improvement of the biochemical parameters including Liver and Renal function enzymes, coagulopathy factors of AFLP patients.

Methods

We searched PubMed, Ovid MEDLINE, Cochrane, CINAHL and Scopus, ClinicalTrials.gov. RevMan statistical software was used for meta-analysis.

Results

Pooled survival proportion for AFLP patients treated with PP/PE was 87.74% (95% CI: 82.84 to 91.65). Efficacy of PP/PE was studied by its effect on mortality. PE/PP was associated with the reduction in the mortality with pooled odds ratio of 0.51 (95% CI: 0.08 to 3.09) with I2 = 86%. Sensitivity analysis after excluding outlier study, yielded a pooled odds ratio of 0.19 (95% CI: 0.02 to 1.52) with reduced heterogeneity (I2 = 63%). Biochemical parameter analysis demonstrated significant improvement post-PP/PE treatment, including decreased bilirubin (MD: 8.30, 95% CI: 6.75 to 9.84), AST (MD: 107.25, 95% CI: 52.45 to 162.06), ALT (MD: 111.08, 95% CI: 27.18 to 194.97), creatinine (MD: 1.66, 95% CI: 1.39 to 1.93), and Prothrombin time (MD: 5.08, 95% CI: 2.93 to 7.22).

Discussion

Despite some heterogeneity, PP/PE shows promise in improving biochemical parameters in AFLP patients. PE can serve as a therapeutic approach for AFLP particularly in severe or refractory cases. PE provides the time for organ to recover and helps in creating a homeostatic environment for liver. Large RCTs and propensity matched studies are needed to better understand the safety and efficacy of the treatment.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022315698.
Glycerol-derived reuterin regulates human intestinal microbiota and metabolites
Authors: Yang Xi , Liu Wei , Zhang Xiaoling , Sun Minhua , Yi Hongbo , Liao Shenquan , Xiang Rong , Zhang Hao , Yang Qiao , Mori Hirotada
Published: October 2024

Reuterin, a mixture of different forms of 3-hydroxypropanal (3-HPA), including HPA hydrate and HPA dimer, is an antimicrobial compound converted from glycerol by Lactobacillus reuteri and other strains. Although its antimicrobial function may be related to its interaction with thiol groups, its temperature stability and effect on the gut environment remain unclear. The present study evaluated the antimicrobial effects and activity of reuterin against Escherichia coli and Salmonella typhimurium. Utilization of a reliable in vitro gut microbiome fermentation system revealed that reuterin has a modulatory effect on the gut microbial community. Reuterin treatment completely inhibited H2 and NH3 production in the gut and significantly enhanced the synthesis of branched short-chain fatty acids. 16s rRNA sequencing indicated that reuterin promoted the growth of Proteobacteria and Bacteroidetes in the in vitro system and significantly modulated gut microbiota composition.
Association of glymphatic system dysfunction with cognitive impairment in temporal lobe epilepsy
Authors: Wang Jiajia , Xia Xiaona , Zhang Bin , Ma Xiaotian , Shi Feng , Wei Ying , Li Ling , Meng Xiangshui
Published: October 2024

Objectives

To explore the relationship between glymphatic dysfunction and cognitive impairment in unilateral temporal lobe epilepsy (TLE).

Methods

This study retrospectively included 38 patients with unilateral TLE and 26 age- and gender-matched healthy controls (HCs). The diffusion tensor image analysis along the perivascular space (DTI-ALPS) index, choroid plexus volume (CPV), and cognitive assessment were obtained for each participant. Neuropsychological test batteries included Montreal Cognitive Assessment (MoCA), Minimum Mental State Examination, Arithmetic Test (AT), Digit Symbol Substitution Test (DSST), Digit Span Test (DST), Boston Naming Test, Block design, Phonological Fluency Test (PFT), and Semantic Verbal Fluency (SVF).

Results

Compared to HCs, TLE patients had lower scores of MoCA, AT, DSST, DST, Block design, PFT and SVF (all p < 0.05) and lower values of mean DTI-ALPS index (1.491 ± 0.142 vs. 1.642 ± 0.123, p < 0.001). Significantly lower DTI-ALPS index values were observed in the ipsilateral hemisphere than in the contralateral hemisphere (1.466 ± 0.129 vs. 1.517 ± 0.175, p = 0.013) for patients with unilateral TLE. Correlation analyses found that SVF performance was significantly or borderline significantly associated with glymphatic function (FDR-corrected p < 0.05 for all DTI-ALPS index and FDR-corrected p = 0.057 for CPV) in TLE patients. Linear regression analyses showed that increased CPV and decreased DTI-ALPS index were independent risk factors for semantic fluency impairment (all p < 0.05). Furthermore, mediation analyses found the mediator role of the mean DTI-ALPS index in the relationship between choroid plexus enlargement and semantic fluency impairment (indirect effect: β = −0.182, 95%CI = −0.486 to −0.037).

Conclusion

These findings reveal the important role of the DTI-ALPS index and CPV in SVF performance in unilateral TLE. Decreased DTI-ALPS index and increased CPV are the independent risk factors for semantic fluency impairment. The DTI-ALPS index may fully mediate the relationship between CP enlargement and SVF performance. These insights provide a radiological foundation for further investigations into the mechanism of the glymphatic system in TLE pathophysiology.
Inside CKD: a microsimulation modelling study projects the clinical and economic burden of chronic kidney disease in Hungary
Authors: Szabó Lilla , Halmai Luca Adél , Ladányi Erzsébet , Garcia Sanchez Juan Jose , Barone Salvatore , Cabrera Claudia , Retat Lise , Webber Laura , Wittmann István , Laczy Boglárka
Published: October 2024

Objectives

The Inside CKD programme implemented a microsimulation modelling approach to project the clinical and economic burden of chronic kidney disease (CKD) between 2024 and 2027 in Hungary.

Methods

Using the peer-reviewed Inside CKD microsimulation, a virtual Hungarian population was generated that was derived from national records, local demographic data and published epidemiological data. These inputs defined the likelihood of a change in health state for each individual as they progressed through the model in annual increments. Individual CKD status, including disease progression, cardiorenal complications and associated costs, was tracked annually to generate the population-level projections of the clinical and economic burden of CKD.

Results

By 2027, people with CKD were projected to constitute 13.3% of the Hungarian national population. The prevalence of heart failure, myocardial infarction and stroke in people with CKD were projected to remain consistently high, reaching 323 447, 69 188 and 120 118 by 2027, respectively. Kidney replacement therapy cases were predicted to remain high at 20 515 in 2024 and 22 325 in 2027, with associated costs increasing from 71.4 billion HUF in 2024 to 79.6 billion HUF in 2027. Total annual healthcare costs associated with treating CKD were projected to constitute 5.4% of the overall national healthcare budget in 2027.

Conclusions

Inside CKD demonstrates that the future burden of CKD in Hungary will be substantial unless current management strategies change. The high prevalence of undiagnosed CKD and associated cardiorenal complications highlight the urgent need for policy interventions focused on early diagnosis and timely intervention to mitigate the future burden of CKD.