bluuebunny/crossref_metadata_2025 huggingface dataset:
A Design Tool for Battery/Supercapacitor Hybrid Energy Storage Systems Based on the Physical–Electrochemical Degradation Battery Model BaSiS
Authors: Shan Weiwei , Schwalm Michael , Shan Martin
Published: July 2024

A design toolbox has been developed for hybrid energy storage systems (HESSs) that employ both batteries and supercapacitors, primarily focusing on optimizing the system sizing/cost and mitigating battery aging. The toolbox incorporates the BaSiS model, a non-empirical physical–electrochemical degradation model for lithium-ion batteries that enables accurate simulations of battery performance and degradation under realistic operating conditions. The paper presents a detailed description of the parameterization, and validation process for the battery model, emphasizing the high accuracy and strong reliability of the battery aging prediction. The HESS design toolbox can be used to investigate the impact of various battery/supercapacitor configurations and energy management algorithms on the design, battery degradation, and system investment cost of the hybrid storage system. To illustrate the effectiveness of the design toolbox, a case study on Dynamic Moderation frequency support in the UK grid was conducted. For this use case, the application of hybrid storage energy systems is well suited due to the highly dynamic power regulation requirements in island grids with low inertia. By utilizing the fast response of supercapacitors, the stress on the battery caused by short-term high-power peaks can be significantly alleviated. In this way, the hybrid storage system effectively reduces either the battery size or the battery aging rate. In summary, this research highlights the crucial role of a comprehensive analysis in the design of hybrid energy storage systems, addressing both battery aging and overall system costs. The design toolbox can provide transparency regarding the design space and assist in determining the most suitable HESS configuration for a given application.
A Productivity Prediction Method of Fracture-Vuggy Reservoirs Based on the PSO-BP Neural Network
Authors: Tian Kunming , Kang Zhihong , Kang Zhijiang
Published: July 2024

Reservoir productivity prediction is a key component of oil and gas field development, and the rapid and accurate evaluation of reservoir productivity plays an important role in evaluating oil field development potential and improving oil field development efficiency. Fracture-vuggy reservoirs are characterized by strong heterogeneity, complex distribution, and irregular development, causing great difficulties in the efficient prediction of fracture-vuggy reservoirs’ productivity. Therefore, a PSO-BP fracture-vuggy reservoir productivity prediction model optimized by feature optimization was proposed in this paper. The Chatterjee correlation coefficient was used to select the appropriate combination of seismic attributes as the input of the prediction model, and we applied the PSO-BP model to predict oil wells’ production in a typical fracture-vuggy reservoir area of Tahe Oilfield, China, with the selected seismic attributes and compared the accuracy with that provided by the BP neural network, linear support vector machine, and multiple linear regression. The prediction results using the four models based on the test set showed that compared with the other three models, the MSE of the PSO-BP model increased by 23% to 62%, the RMSE increased by 12 to 38 percent, the MAE increased by 18 to 44 percent, the SSE increased by 23 to 62 percent, and the R-square value increased by 2 to 13 percent. This comparison proves that the PSO-BP neural network model proposed in this paper is suitable for the productivity prediction of fracture-vuggy reservoirs and has better performance, which is of guiding significance for the development and production of fracture-vuggy reservoirs.
Robust Control Design of Under-Actuated Nonlinear Systems: Quadcopter Unmanned Aerial Vehicles with Integral Backstepping Integral Terminal Fractional-Order Sliding Mode
Authors: Ullah Safeer , Alghamdi Hisham , Algethami Abdullah A. , Alghamdi Baheej , Hafeez Ghulam
Published: July 2024

In this paper, a novel robust finite-time control scheme is specifically designed for a class of under-actuated nonlinear systems. The proposed scheme integrates a reaching phase-free integral backstepping method with an integral terminal fractional-order sliding mode to ensure finite-time stability at the desired equilibria. The core of the algorithm is built around proportional-integral-based nonlinear virtual control laws that are systematically designed in a backstepping manner. A fractional-order integral terminal sliding mode is introduced in the final step of the design, enhancing the robustness of the overall system. The robust nonlinear control algorithm developed in this study guarantees zero steady-state errors at each step while also providing robustness against matched uncertain disturbances. The stability of the control scheme at each step is rigorously proven using the Lyapunov candidate function to ensure theoretical soundness. To demonstrate the practicality and benefits of the proposed control strategy, simulation results are provided for two systems: a cart–pendulum system and quadcopter UAV. These simulations illustrate the effectiveness of the proposed control scheme in real-world scenarios. Additionally, the results are compared with those from the standard literature to highlight the superior performance and appealing nature of the proposed approach for underactuated nonlinear systems. This comparison underscores the advantages of the proposed method in terms of achieving robust and stable control in complex systems.
Existence, Uniqueness, and Stability of a Nonlinear Tripled Fractional Order Differential System
Authors: Madani Yasir A. , Rabih Mohammed Nour A. , Alqarni Faez A. , Ali Zeeshan , Aldwoah Khaled A. , Hleili Manel
Published: July 2024

This manuscript investigates the existence, uniqueness, and different forms of Ulam stability for a system of three coupled differential equations involving the Riemann–Liouville (RL) fractional operator. The Leray–Schauder alternative is employed to confirm the existence of solutions, while the Banach contraction principle is used to establish their uniqueness. Stability conditions are derived utilizing classical nonlinear functional analysis techniques. Theoretical findings are illustrated with an example. The proposed system generalizes third-order ordinary differential equations (ODEs) with different boundary conditions (BCs).
Assessment of Pregnant Women’s Knowledge and Perceptions of Antenatal Ultrasound in Saudi Arabia
Authors: Alghamdi Sami , Dhahi Najwa , Gashash Fahad , Abuturboush Ghasan , Hazzazi Afaf , Alhailiy Ali , Alashban Yazeed
Published: July 2024

Background: This study aims to evaluate pregnant women’s knowledge of antenatal ultrasound in Saudi Arabia and its correlation with demographic factors like age and education to enhance prenatal care. Methods: A cross-sectional study was conducted in six Saudi Arabian hospitals, involving 22 questions split between sociodemographic information and knowledge of antenatal ultrasound. Descriptive statistics were used to characterize the participants’ demographics and responses. Additionally, inferential statistics were employed to analyze the relationships and differences among the study variables. Results: Among the 531 pregnant women in the study, most demonstrated a good understanding of antenatal ultrasound, identifying its various uses. Specifically, they recognized its roles in evaluating fetal growth (82.5%), placental location (81.7%), amniotic fluid volume (67%), and fetal morphology (65%), predicting the delivery date (79%), and determining the baby’s sex (89%). A majority viewed ultrasound as important (89.3%), safe (82.3%), and tolerable (76.3%) for prenatal care. Additionally, 66.7% felt adequately informed, mainly through clinical staff and doctors. Younger age, lower education, lack of prior ultrasound experience, and first pregnancy were linked to lower knowledge. Approximately 65% were uncertain about the nonionizing radiation properties of ultrasound. Conclusions: The study found that while most pregnant women in Saudi Arabia understand the objectives of antenatal ultrasonography, there are gaps in their knowledge about its nonionizing properties. Younger age, lower education, lack of prior ultrasound experience, and first pregnancy contribute to lower knowledge.
A Pathfinding Algorithm for Large-Scale Complex Terrain Environments in the Field
Authors: Kui Luchao , Yu Xianwen
Published: July 2024

Pathfinding for autonomous vehicles in large-scale complex terrain environments is difficult when aiming to balance efficiency and quality. To solve the problem, this paper proposes Hierarchical Path-Finding A* based on Multi-Scale Rectangle, called RHA*, which achieves efficient pathfinding and high path quality for large-scale unequal-weighted maps. Firstly, the original map grid cells were aggregated into fixed-size clusters. Then, an abstract map was constructed by aggregating equal-weighted clusters into rectangular regions of different sizes and calculating the nodes and edges of the regions in advance. Finally, real-time pathfinding was performed based on the abstract map. The experiment showed that the computation time of real-time pathfinding was reduced by 96.64% compared to A* and 20.38% compared to HPA*. The total cost of the generated path deviated no more than 0.05% compared to A*. The deviation value is reduced by 99.2% compared to HPA*. The generated path can be used for autonomous vehicle traveling in off-road environments.
A Lightweight Multi-Label Classification Method for Urban Green Space in High-Resolution Remote Sensing Imagery
Authors: Lin Weihua , Zhang Dexiong , Liu Fujiang , Guo Yan , Chen Shuo , Wu Tianqi , Hou Qiuyan
Published: July 2024

Urban green spaces are an indispensable part of the ecology of cities, serving as the city’s “purifier” and playing a crucial role in promoting sustainable urban development. Therefore, the refined classification of urban green spaces is an important task in urban planning and management. Traditional methods for the refined classification of urban green spaces heavily rely on expert knowledge, often requiring substantial time and cost. Hence, our study presents a multi-label image classification model based on MobileViT. This model integrates the Triplet Attention module, along with the LSTM module, to enhance its label prediction capabilities while maintaining its lightweight characteristic for standalone operation on mobile devices. Trial outcomes in our UGS dataset in this study demonstrate that the approach we used outperforms the baseline by 1.64%, 3.25%, 3.67%, and 2.71% in mAP,F1,precision, and recall, respectively. This indicates that the model can uncover the latent dependencies among labels to enhance the multi-label image classification device’s performance. This study provides a practical solution for the intelligent and detailed classification of urban green spaces, which holds significant importance for the management and planning of urban green spaces.
Integrating NoSQL, Hilbert Curve, and R*-Tree to Efficiently Manage Mobile LiDAR Point Cloud Data
Authors: Yang Yuqi , Zuo Xiaoqing , Zhao Kang , Li Yongfa
Published: July 2024

The widespread use of Light Detection and Ranging (LiDAR) technology has led to a surge in three-dimensional point cloud data; although, it also poses challenges in terms of data storage and indexing. Efficient storage and management of LiDAR data are prerequisites for data processing and analysis for various LiDAR-based scientific applications. Traditional relational database management systems and centralized file storage struggle to meet the storage, scaling, and specific query requirements of massive point cloud data. However, NoSQL databases, known for their scalability, speed, and cost-effectiveness, provide a viable solution. In this study, a 3D point cloud indexing strategy for mobile LiDAR point cloud data that integrates Hilbert curves, R*-trees, and B+-trees was proposed to support MongoDB-based point cloud storage and querying from the following aspects: (1) partitioning the point cloud using an adaptive space partitioning strategy to improve the I/O efficiency and ensure data locality; (2) encoding partitions using Hilbert curves to construct global indices; (3) constructing local indexes (R*-trees) for each point cloud partition so that MongoDB can natively support indexing of point cloud data; and (4) a MongoDB-oriented storage structure design based on a hierarchical indexing structure. We evaluated the efficacy of chunked point cloud data storage with MongoDB for spatial querying and found that the proposed storage strategy provides higher data encoding, index construction and retrieval speeds, and more scalable storage structures to support efficient point cloud spatial query processing compared to many mainstream point cloud indexing strategies and database systems.
Aspirin Stimulates the Osteogenic Differentiation of Human Adipose Tissue-Derived Stem Cells In Vitro
Authors: Funke Sarah , Wiggenhauser Paul Severin , Grundmeier Anna , Taha Sara , Fuchs Benedikt , Birt Alexandra , Koban Konstantin , Giunta Riccardo E. , Kuhlmann Constanze
Published: July 2024

This study investigates the impact of acetylsalicylic acid (ASA), also known as aspirin, on adipose tissue-derived stem cells (ASCs), aiming to elucidate its dose-dependent effects on morphology, viability, proliferation, and osteogenic differentiation. Isolated and characterized human ASCs were exposed to 0 µM, 100 µM, 200 µM, 400 µM, 800 µM, 1000 µM, 10,000 µM, and 16,000 µM of ASA in vitro. Cell morphology, viability, and proliferation were evaluated with fluorescent live/dead staining, alamarBlue viability reagent, and CyQUANT® cell proliferation assay, respectively. Osteogenic differentiation under stimulation with 400 µM or 1000 µM of ASA was assessed with alizarin red staining and qPCR of selected osteogenic differentiation markers (RUNX2, SPP1, ALPL, BGLAP) over a 3- and 21-day-period. ASA doses ≤ 1000 µM showed no significant impact on cell viability and proliferation. Live/dead staining revealed a visible reduction in viable cell confluency for ASA concentrations ≥ 1000 µM. Doses of 10,000 µM and 16,000 µM of ASA exhibited a strong cytotoxic and anti-proliferative effect in ASCs. Alizarin red staining revealed enhanced calcium accretion under the influence of ASA, which was macro- and microscopically visible and significant for 1000 µM of ASA (p = 0.0092) in quantification if compared to osteogenic differentiation without ASA addition over a 21-day-period. This enhancement correlated with a more pronounced upregulation of osteogenic markers under ASA exposure (ns). Our results indicate a stimulatory effect of 1000 µM of ASA on the osteogenic differentiation of ASCs. Further research is needed to elucidate the precise molecular mechanisms underlying this effect; however, this discovery suggests promising opportunities for enhancing bone tissue engineering with ASCs as cell source.
Comprehensive Analysis of Placental DNA Methylation Changes and Fetal Birth Weight in Pigs
Authors: Tan Baohua , Xiao Liyao , Wang Yongzhong , Zhou Chen , Huang Huijun , Li Zicong , Hong Linjun , Cai Gengyuan , Wu Zhenfang , Gu Ting
Published: July 2024

Birth weight is a complex multifactorial trait relevant to health states and disease risks in later life. The placenta is essential for proper fetal growth and facilitates gas, nutrient, and waste exchange between the mother and developing fetus. How changes in placental DNA methylation affect fetal birth weight remains to be fully elucidated. In this study, we used whole-genome bisulfite sequencing and RNA sequencing to reveal a global map of DNA methylation and gene expression changes between the placentas of highest birth weight and lowest birth weight piglets in the same litters. The transcriptome analysis identified 1682 differential expressed genes and revealed key transcriptional properties in distinct placentas. We also identified key transcription factors that may drive the differences in DNA methylome patterns between placentas. The decrease in DNA methylation level in the promoter was associated with the transcriptional activation of genes associated with angiogenesis, extracellular matrix remodeling, and transmembrane transport. Our results revealed the regulatory role of DNA methylation in gene transcription activity leading to the differences in placental morphological structures and birth weights of piglets. These results could provide novel clues to clarify the underlying regulatory mechanisms of placental development and fetal growth.
Gastrodin Improves the Activity of the Ubiquitin–Proteasome System and the Autophagy–Lysosome Pathway to Degrade Mutant Huntingtin
Authors: Sun He , Li Miao , Li Yunling , Zheng Na , Li Jiaxin , Li Xiang , Liu Yingying , Ji Qianyun , Zhou Liping , Su Jingwen , Huang Wanxu , Liu Zhongbo , Liu Peng , Zou Libo
Published: July 2024

Gastrodin (GAS) is the main chemical component of the traditional Chinese herb Gastrodia elata (called “Tianma” in Chinese), which has been used to treat neurological conditions, including headaches, epilepsy, stroke, and memory loss. To our knowledge, it is unclear whether GAS has a therapeutic effect on Huntington’s disease (HD). In the present study, we evaluated the effect of GAS on the degradation of mutant huntingtin protein (mHtt) by using PC12 cells transfected with N-terminal mHtt Q74. We found that 0.1–100 μM GAS had no effect on the survival rate of Q23 and Q74 PC12 cells after 24–48 h of incubation. The ubiquitin–proteasome system (UPS) is the main system that clears misfolded proteins in eukaryotic cells. Mutated Htt significantly upregulated total ubiquitinated protein (Ub) expression, decreased chymotrypsin-like, trypsin-like and caspase-like peptidase activity, and reduced the colocalization of the 20S proteasome with mHtt. GAS (25 μM) attenuated all of the abovementioned pathological changes, and the regulatory effect of GAS on mHtt was found to be abolished by MG132, a proteasome inhibitor. The autophagy–lysosome pathway (ALP) is another system for misfolded protein degradation. Although GAS downregulated the expression of autophagy markers (LC3II and P62), it increased the colocalization of LC3II with lysosomal associated membrane protein 1 (LAMP1), which indicates that ALP was activated. Moreover, GAS prevented mHtt-induced neuronal damage in PC12 cells. GAS has a selective effect on mHtt in Q74 PC12 cells and has no effect on Q23 and proteins encoded by other genes containing long CAGs, such as Rbm33 (10 CAG repeats) and Hcn1 (>30 CAG repeats). Furthermore, oral administration of 100 mg/kg GAS increased grip strength and attenuated mHtt aggregates in B6-hHTT130-N transgenic mice. This is a high dose (100 mg/kg GAS) when compared with experiments on HD mice with other small molecules. We will design more doses to evaluate the dose–response relationship of the inhibition effect of GAS on mHtt in our next study. In summary, GAS can promote the degradation of mHtt by activating the UPS and ALP, making it a potential therapeutic agent for HD.
Neuroblastoma—A Review of Combination Immunotherapy
Authors: Pieniążek Barbara , Cencelewicz Katarzyna , Bździuch Patrycja , Młynarczyk Łukasz , Lejman Monika , Zawitkowska Joanna , Derwich Katarzyna
Published: July 2024

Neuroblastoma is the most common extracranial solid tumor found in childhood and is responsible for 15% of deaths among children with cancer. Although multimodal therapies focused on surgery, chemotherapy, radiotherapy, and stem cell transplants have favorable results in many cases, the use of conventional therapies has probably reached the limit their possibility. Almost half of the patients with neuroblastoma belong to the high-risk group. Patients in this group require a combination of several therapeutic approaches. It has been shown that various immunotherapies combined with conventional methods can work synergistically. Due to the development of such therapeutic methods, we present combinations and forms of combining immunotherapy, focusing on their mechanisms and benefits but also their limitations and potential side effects.
TLR2 and TLR4 Are Expressed in Epiretinal Membranes: Possible Links with Vitreous Levels of Complement Fragments and DAMP-Related Proteins
Authors: Dinice Lucia , Esposito Graziana , Cacciamani Andrea , Balzamino Bijorn Omar , Cosimi Pamela , Cafiero Concetta , Ripandelli Guido , Micera Alessandra
Published: July 2024

Previous studies reported the expression of toll-like receptors (TLRs), merely TLR2 and TLR4, and complement fragments (C3a, C5b9) in vitreoretinal disorders. Other than pathogens, TLRs can recognize endogenous products of tissue remodeling as damage-associated molecular pattern (DAMPs). The aim of this study was to confirm the expression of TLR2 and TLR4 in the fibrocellular membranes and vitreal fluids (soluble TLRs) of patients suffering of epiretinal membranes (ERMs) and assess their association with disease severity, complement fragments and inflammatory profiles. Twenty (n = 20) ERMs and twelve (n = 12) vitreous samples were collected at the time of the vitrectomy. Different severity-staged ERMs were processed for: immunolocalization (IF), transcriptomic (RT-PCR) and proteomics (ELISA, IP/WB, Protein Chip Array) analysis. The investigation of targets included TLR2, TLR4, C3a, C5b9, a few selected inflammatory biomarkers (Eotaxin-2, Rantes, Vascular Endothelial Growth Factor (VEGFA), Vascular Endothelial Growth Factor receptor (VEGFR2), Interferon-γ (IFNγ), Interleukin (IL1β, IL12p40/p70)) and a restricted panel of matrix enzymes (Matrix metalloproteinases (MMPs)/Tissue Inhibitor of Metallo-Proteinases (TIMPs)). A reduced cellularity was observed as function of ERM severity. TLR2, TLR4 and myD88 transcripts/proteins were detected in membranes and decreased upon disease severity. The levels of soluble TLR2 and TLR4, as well as C3a, C5b9, Eotaxin-2, Rantes, VEGFA, VEGFR2, IFNγ, IL1β, IL12p40/p70, MMP7 and TIMP2 levels were changed in vitreal samples. Significant correlations were observed between TLRs and complement fragments and between TLRs and some inflammatory mediators. Our findings pointed at TLR2 and TLR4 over-expression at early stages of ERM formation, suggesting the participation of the local immune response in the severity of disease. These activations at the early-stage of ERM formation suggest a potential persistence of innate immune response in the early phases of fibrocellular membrane formation.
Computational NMR Study of Benzothienoquinoline Heterohelicenes
Authors: Semenov Valentin A. , Martin Gary E. , Krivdin Leonid B.
Published: July 2024

Early NMR studies of several heterohelicenes containing an annular nitrogen atom and a thiophene ring in their structure suggested the possibility of the lengthening of the carbon–carbon bonds in the interior of the helical turn of the molecule based on the progressive upfield shift of 13C resonances toward the center of the helical turn. We now report a comprehensive analysis of the optimized geometry and a comparison of the calculated vs. observed 1H and 13C NMR chemical shifts of nineteen representative benzothienoquinoline heterohelicenes. As was initially hypothesized on the basis of the progressive upfield shift of carbon resonances toward the center of the interior helical turn, the present computational study has demonstrated that carbon–carbon bonds indeed have more sp3 character and are longer than normal sp2 bonds to accommodate the helical twist of the molecule, as expected.
Effect of Solvent Properties on the Critical Solution Temperature of Thermoresponsive Polymers
Authors: Beitl Konstantin Nikolaus , Reimhult Erik
Published: July 2024

The ability of thermoresponsive polymers to respond to temperature with a reversible conformational change makes them promising ‘smart’ materials for solutions in medical and biotechnological applications. In this work, two such polymers and structural isomers were studied: poly(N-isopropyl acrylamide) (PNiPAm) and poly(2-isopropyl-2-oxazoline) (PiPOx). We compare the critical solution temperatures (CST) of these polymers in D2O and H2O in the presence of Hofmeister series salts, as results obtained under these different solvent conditions are often compared. D2O has a higher dipole moment and electronegativity than H2O, which could significantly alter the CST transition. We used two complementary methods to measure the CST, dynamic light scattering (DLS) and differential scanning calorimetry (DSC) and found that the CST decreased significantly in D2O compared to H2O. In the presence of highly concentrated kosmotropes, the CST of both polymers decreased in both solvents. The influence of the kosmotropic anions was smaller than the water isotope effect at low ionic strengths but considerably higher at physiological ionic strengths. However, the Hofmeister anion effect was quantitatively different in H2O than in D2O, with the largest relative differences observed for Cl−, where the CSTs in D2O decreased more than in H2O measured by DLS but less by DSC. PiPOx was more sensitive than PNiPAm to the presence of chaotropes. It exhibited much higher transition enthalpies and multistep transitions, especially in aqueous solutions. Our results highlight that measurements of thermoresponsive polymer properties in D2O cannot be compared directly or quantitatively to application conditions or even measurements performed in H2O.
Developments in Alloplastic Bone Grafts and Barrier Membrane Biomaterials for Periodontal Guided Tissue and Bone Regeneration Therapy
Authors: Ashfaq Rabia , Kovács Anita , Berkó Szilvia , Budai-Szűcs Mária
Published: July 2024

Periodontitis is a serious form of oral gum inflammation with recession of gingival soft tissue, destruction of the periodontal ligament, and absorption of alveolar bone. Management of periodontal tissue and bone destruction, along with the restoration of functionality and structural integrity, is not possible with conventional clinical therapy alone. Guided bone and tissue regeneration therapy employs an occlusive biodegradable barrier membrane and graft biomaterials to guide the formation of alveolar bone and tissues for periodontal restoration and regeneration. Amongst several grafting approaches, alloplastic grafts/biomaterials, either derived from natural sources, synthesization, or a combination of both, offer a wide variety of resources tailored to multiple needs. Examining several pertinent scientific databases (Web of Science, Scopus, PubMed, MEDLINE, and Cochrane Library) provided the foundation to cover the literature on synthetic graft materials and membranes, devoted to achieving periodontal tissue and bone regeneration. This discussion proceeds by highlighting potential grafting and barrier biomaterials, their characteristics, efficiency, regenerative ability, therapy outcomes, and advancements in periodontal guided regeneration therapy. Marketed and standardized quality products made of grafts and membrane biomaterials have been documented in this work. Conclusively, this paper illustrates the challenges, risk factors, and combination of biomaterials and drug delivery systems with which to reconstruct the hierarchical periodontium.
Agrobacterium tumefaciens-Mediated Genetic Transformation of Eclipta alba
Authors: Aggarwal Diwakar , Datta Vasudha , Tuli Hardeep Singh , Kumar Pawan , Ramniwas Seema
Published: July 2024

Eclipta alba (Linn.) Hassk. (Asteraceae) is a high value medicinal plant which possesses diverse medicinal properties. It is an important herb for the treatment of various disorders, and is primarily used as a hepatoprotectant. Its major biochemical constituents include wedelolactone and dimethyl-wedelolactone (coumestans), which possess anti-hepatotoxic properties. Due to its numerous medicinal properties, it is in high demand by the pharmaceutical industry and therefore requires urgent biotechnological interventions for its improvement. Therefore, the present study was constructed with the aim of developing an efficient genetic transformation protocol for E. alba, which will help in the mass production of the active compounds found in E. alba. Agrobacterium tumefaciens strain LBA 4404, containing vector pBI121, was used to genetically transform the plant, and the effect of various factors such as infection type, light cycle effect, effect of pH, among others, on the genetic transformation efficiency was analyzed. Regenerated transformed shoots were confirmed using the standard Polymerase Chain Reaction PCR method. Kanamycin-resistant and beta- glucurosidaseGUS-positive shoots indicated the development of transgenic shoots in E. alba. Amplification of nptll and uidA genes confirmed the integration of t-DNA transgenic shoots. In conclusion, various factors affecting the transformation efficiency were analyzed, and a reliable A. tumefaciens-mediated genetic transformation protocol was developed.
Gender Differences in Survival after Coronary Artery Bypass Grafting—13-Year Results from KROK Registry
Authors: Hirnle Grzegorz , Stankiewicz Adrian , Mitrosz Maciej , Aboul-Hassan Sleiman Sebastian , Deja Marek , Rogowski Jan , Cichoń Romuald , Anisimowicz Lech , Bugajski Paweł , Tobota Zdzisław , Maruszewski Bohdan , Hrapkowicz Tomasz
Published: July 2024

The influence of gender on both early and long-term outcomes of coronary artery bypass grafting (CABG) is not clearly defined. Objectives: This study aimed to assess the impact of gender on early and long-term mortality after CABG using data from the KROK Registry. Methods: All 133,973 adult patients who underwent CABG in Poland between 1 January 2009 and 31 December 2019 were included in the Polish National Registry of Cardiac Surgical Procedures (KROK Registry). The study enrolled 90,541 patients: 68,401 men (75.55%) and 22,140 women (24.45%) who met the inclusion criteria. Then, 30-day mortality, 1-year mortality, and long-term mortality rates were compared. Results: Advanced age, higher Canadian Cardiovascular Society (CCS) and New York Heart Association (NYHA) grade, diabetes, hypercholesterolemia, arterial hypertension, body mass index BMI > 35 kg/m2, and renal failure, before the propensity matching, were more frequently observed in women. Women more frequently underwent urgent surgery, including single and double graft surgery, and off-pump CABG (OPCAB) (p < 0.001). In propensity-matched groups, early mortality (30 days) was significantly higher in women (3.4% versus 2.8%, p < 0.001). The annual mortality remained higher in this group (6.6% versus 6.0%, p = 0.025). However, long-term mortality differed significantly between the groups and was higher in the male group (33.0% men versus 28.8% women, p < 0.001). Conclusions: There are no apparent differences in long-term mortality between the two sexes in the entire population. In propensity-matched patients, early mortality was lower for men, but the long-term survival was found to be better in women.
Innovations in Intracoronary Imaging: Present Clinical Practices and Future Outlooks
Authors: Mitsis Andreas , Eftychiou Christos , Kadoglou Nikolaos P. E. , Theodoropoulos Konstantinos C. , Karagiannidis Efstratios , Nasoufidou Athina , Ziakas Antonios , Tzikas Stergios , Kassimis George
Published: July 2024

Engaging intracoronary imaging (IC) techniques such as intravascular ultrasound or optical coherence tomography enables the precise description of vessel architecture. These imaging modalities have well-established roles in providing guidance and optimizing percutaneous coronary intervention (PCI) outcomes. Furthermore, IC is increasingly recognized for its diagnostic capabilities, as it has the unique capacity to reveal vessel wall characteristics that may not be apparent through angiography alone. This manuscript thoroughly reviews the contemporary landscape of IC in clinical practice. Focused on current methodologies, the review explores the utility and advancements in IC techniques. Emphasizing their role in clarifying coronary pathophysiology, guiding PCI, and optimizing patient outcomes, the manuscript critically evaluates the strengths and limitations of each modality. Additionally, the integration of IC into routine clinical workflows and its impact on decision-making processes are discussed. By synthesizing the latest evidence, this review provides valuable insights for clinicians, researchers, and healthcare professionals involved in the dynamic field of interventional cardiology.
Is a Low Dosage of Medical Cannabis Effective for Treating Pain Related to Fibromyalgia? A Pilot Study and Systematic Review
Authors: Giardina Antonio , Palmieri Rocco , Ponticelli Maria , Antonelli Carlo , Carlucci Vittorio , Colangelo Monica , Benedetto Nadia , Di Fazio Aldo , Milella Luigi
Published: July 2024

Background and Objectives: Fibromyalgia is a multifaceted and frequently misunderstood chronic pain disease marked by widespread musculoskeletal pain and cognitive/somatic dysfunction. This trial aims to contribute to the existing knowledge on treating fibromyalgia (FM) with medical cannabis (Cannabis sativa L.) and explore a safer and more effective cannabis administration method. The goal is to provide evidence-based findings that can guide alternative treatment options for FM patients by assessing a pilot study. Materials and Methods: The trial was performed at the pain therapy unit of the San Carlo Hospital (Potenza, Italy) by administrating to 30 FM patients 100 mg/day of Bedrocan® (Bedrocan International, Veendam, The Netherlands) as a decoction. The Numerical Rating Scale (NRS) and SF-12 short-form health questionnaire were used to evaluate pain intensity and the quality of life at the beginning of the study and the 6th-month follow-up. A systematic review of all clinical studies investigating the use of cannabis to reduce FM was also undertaken to place this study in the context of the existing evidence base. Results: Pain intensity evaluated with the NRS lowered from a median of 8 [95% CI 7.66–8.54] at a baseline to a median of 4 (95% CI 3.28–4.79) after 6 months of follow-up (p-value < 0.001; t-test). Similarly, significant physical and mental state improvement, evaluated with the SF-12 questionnaire, was found in 96.67% and 82.33% of patients, respectively (95% CI 44.11–51.13 for the physical state, and 53.48–58.69 for mental state assessed after the 6th-month follow-up; p-value < 0.001; t-test). The systematic analysis of the literature identified 10 clinical trials concerning the treatment of fibromyalgia with cannabis. Conclusions: Considering results from the present pilot study and systematic review, it is possible to assume that medical cannabis may be considered an alternative therapy for FM patients who do not respond to conventional pharmacological therapy.
Histologic Evaluation of Early Papilla Healing after Augmentation with Injectable Hyaluronic Acid—A Proof of Concept
Authors: Vela Octavia Carolina , Boariu Marius Ion , Iorio-Siciliano Vincenzo , Vaduva Adrian , Belova Alla , Stratul Stefan-Ioan , Rusu Darian
Published: July 2024

Objectives: This human histological study’s purpose was to histologically evaluate papillae’s healing after hyaluronic acid (HA) gel augmentation at three healing time points after one injection with hyaDENT BG®. Methods: Fifteen papillae from two patients with stage III, grade B periodontitis have been selected for this study. Every week for three weeks, five papillae were injected once with HA gel, and during the fourth week, the papillae were surgically removed as part of step 3 of the periodontal treatment. The histological analysis was performed on fifteen papillae, with five papillae corresponding to every timepoint of healing (weeks 1, 2, and 3). The primary outcome was considered to be the newly formed collagen fibers. The presence of residual HA, the integrity of epithelium or the presence of erosions/ulcerations, the presence and characteristics of inflammatory infiltrate, the presence of granulomatous reactions, and interstitial edema were considered to be secondary outcomes. Results: From the first to the third week, newly formed connective tissue begins to appear, while the observed HA pools (vesicles) content decreases. The density of inflammatory infiltrate was higher in the first week after injection, decreasing considerably by week 3; however, it was still visible throughout the healing time points. A granulomatous reaction was present in only three samples, while no signs of ulceration or necrosis could be observed; however, epithelial erosions could be observed on some samples after the first week. Conclusions: Papila augmentation with hyaluronic acid promotes new collagen formation from the second week of healing despite some foreign body granulomatous reactions.
Progress of Ossification after Mandibular Reconstruction by Free Fibula Flap Depending on Different Timing of Radiotherapy: A Retrospective 3D Analysis by CT Scans
Authors: Gottsauner Maximilian , Sroka Anne Marie , Eichberger Jonas , Schuderer Johannes , Zeman Florian , Fiedler Mathias , Maurer Michael , Einspieler Ingo , Reichert Torsten E. , Ettl Tobias
Published: July 2024

Background: The aim of this study was to evaluate the difference between pre- and post-operative radiotherapy on the progress of ossification after free fibula flap reconstruction of the mandible using three-dimensional (3D) analysis. Methods: A total of 38 free fibula reconstructions of the mandible were evaluated retrospectively for ossification between bone segments by measuring Hounsfield Units (HU) in at least two postoperative computer tomography scans (average of 2.4 scans per patient; around the 5th, 12th, 16th, and 19th month postoperative). Three subgroups were created according to the time of irradiation: preoperative radiotherapy (preORT) (n = 11), postoperative radiotherapy (postORT) (n = 16), and patients without any radiation therapy (n = 11) as the control group (noRT). HU in eight regions of interest (ROI) and overlapping surfaces between segments per contact point, as well as influencing factors, were analyzed. Results: The fastest progress in gain of HU ossification with a difference of 0.30 HU/day was observed in noRT compared to preORT (p = 0.002). postORT was −0.24 HU/day slower than preORT (p = 0.005). Original and grafted bone showed a significantly slower HU uptake than between two graft segments with −84.18 HU/day (p < 0.001). Moreover, a larger initial overlapping surface between the segments in cm2 resulted in a higher rise of HU/day (p < 0.001). Conclusions: 3D analysis of post-reconstructive CT scans shows prolonged ossification of mandible reconstructions by free fibula after head and neck radiation. The effect is distinct in cases with post-operative adjuvant radiotherapy. The effects of radiotherapy on ossification may be minimized by a larger initial contact surface and improved operational techniques. Moreover, HU longitudinal measurements and 3D analysis offer new perspectives for clinical evaluation of successful bony healing.
Renal Transplant Outcomes in Plasma Cell Dyscrasias and AL Amyloidosis after Treatment with Daratumumab
Authors: Mohidin Barian , Needleman Amy , Fernando Raymond , Lowe David M. , Wechalekar Ashutosh , Sheaff Michael , Salama Alan , Jones Gareth
Published: July 2024

Background: The morbidity and mortality from AL amyloidosis has significantly improved with the development of novel treatments. Daratumumab is a highly effective treatment for AL amyloidosis, but end-stage kidney disease is a common complication of this condition. Kidney transplantation is the ideal form of renal replacement therapy but has historically been contraindicated in this group of patients. Methods: Given the improved survival and better treatments of both conditions, we argue that it is time to reconsider transplanting these patients. Results: We report our experience of transplanting four patients with AL amyloidosis who had achieved stable remission through treatment with daratumumab. Conclusions: We highlight the key challenges involved and discuss important clinical issues for patients receiving daratumumab, particularly the difficulties with interpreting the crossmatch in light of daratumumab and immunoglobulin therapy interference. We also discuss the complexities involved in balancing the risks of infection, relapse, rejection, and immunosuppression in such patients.
Unveiling Silent Consequences: Impact of Pulmonary Tuberculosis on Lung Health and Functional Wellbeing after Treatment
Authors: Bansal Nidhi , Arunachala Sumalatha , Kaleem Ullah Mohammed , Kulkarni Shreedhar , Ravindran Sukanya , ShankaraSetty Rekha Vaddarahalli , Malamardi Sowmya , Chaya Sindaghatta Krishnarao , Lokesh Komarla Sundararaja , Parthasarathi Ashwaghosha , Shyam Prasad Shetty Bellipady , Chikkahonnaiah Prashanth , Vishwanath Prashant , Siddaiah Jayaraj Biligere , Mahesh Padukudru Anand
Published: July 2024

Background: Pulmonary tuberculosis (TB) remains a major public health issue in India, with high incidence and mortality. The current literature on post-TB sequelae functional defects focuses heavily on spirometry, with conflicting obstruction vs. restriction data, lacks advanced statistical analysis, and has insufficient data on diffusion limitation and functional impairment. Objective: This study aimed to thoroughly evaluate post-tubercular sequelae after treatment, assessing chest radiology, spirometry, diffusing capacity, and exercise capacity. Methods: A total of 85 patients were studied at a university teaching hospital in Mysuru. The data collected included characteristics, comorbidities, smoking history, and respiratory symptoms. The investigations included spirometry, DLCO, chest X-rays with scoring, and 6MWT. Results: Of the patients, 70% had abnormal X-rays post-treatment, correlating with reduced lung function. Additionally, 70% had impaired spirometry with obstructive/restrictive patterns, and 62.2% had reduced DLCO, with females at higher risk. Smoking increased the risk of sequelae. Conclusions: Most patients had residual radiological/lung function abnormalities post-treatment. Advanced analyses provide insights into obstructive vs. restrictive defects. Ongoing research should explore pathogenetic mechanisms and therapeutic modalities to minimize long-term post-TB disability.
Endodontic Treatment Outcomes in Cone Beam Computed Tomography Images—Assessment of the Diagnostic Accuracy of AI
Authors: Kazimierczak Wojciech , Kazimierczak Natalia , Issa Julien , Wajer Róża , Wajer Adrian , Kalka Sandra , Serafin Zbigniew
Published: July 2024

Background/Objectives: The aim of this study was to assess the diagnostic accuracy of the AI-driven platform Diagnocat for evaluating endodontic treatment outcomes using cone beam computed tomography (CBCT) images. Methods: A total of 55 consecutive patients (15 males and 40 females, aged 12–70 years) referred for CBCT imaging were included. CBCT images were analyzed using Diagnocat’s AI platform, which assessed parameters such as the probability of filling, adequate obturation, adequate density, overfilling, voids in filling, short filling, and root canal number. The images were also evaluated by two experienced human readers. Diagnostic accuracy metrics (accuracy, precision, recall, and F1 score) were assessed and compared to the readers’ consensus, which served as the reference standard. Results: The AI platform demonstrated high diagnostic accuracy for most parameters, with perfect scores for the probability of filling (accuracy, precision, recall, F1 = 100%). Adequate obturation showed moderate performance (accuracy = 84.1%, precision = 66.7%, recall = 92.3%, and F1 = 77.4%). Adequate density (accuracy = 95.5%, precision, recall, and F1 = 97.2%), overfilling (accuracy = 95.5%, precision = 86.7%, recall = 100%, and F1 = 92.9%), and short fillings (accuracy = 95.5%, precision = 100%, recall = 86.7%, and F1 = 92.9%) also exhibited strong performance. The performance of AI for voids in filling detection (accuracy = 88.6%, precision = 88.9%, recall = 66.7%, and F1 = 76.2%) highlighted areas for improvement. Conclusions: The AI platform Diagnocat showed high diagnostic accuracy in evaluating endodontic treatment outcomes using CBCT images, indicating its potential as a valuable tool in dental radiology.
Survey on Parkinson’s Disease Diagnosis Impact: Patients, Caregivers and Health Care Professionals’ Perspectives
Authors: Dodaj Stela , Fabbri Margherita , Doe de Maindreville Anne , Foubert-Samier Alexandra , Toussaint Marie-Claire , Carriere Nicolas , Lopez Jeanne , Giroud Marine , Sattler Virginie , Gerdelat Angélique , Baille Guillaume , Turc Jean Denis , Barthelemy Christel , Scotto d Apollonia Charlotte , Fabre Marie Helene , Eytier Elina , Thiriez Claire , Fluchère Frederique , Ory-Magne Fabienne
Published: July 2024

Background: The announcement of Parkinson’s disease (PD) diagnosis may provoke negative feelings that impact the ability to cope with the disease and all life changes related to this new condition. There are scarce data on how to improve communication about PD diagnosis and which factors may influence this outcome. Methods: We performed a national French survey, investigating the diagnosis announcement impact on a large population of people living with PD (PwPD), who recently received the diagnosis (≤1 year since PD diagnosis), and on related caregivers and health care professionals (HCPs), from tertiary and community-based hospitals. Results: A total of 397 PwPD (45% female and 82% > 50 years old), 192 caregivers and 120 HCPs (69% neurologists) completed the questionnaire. The diagnosis was not expected by about 60% of PwPD and induced negative feelings in the majority (82%) of them. Negative feelings that PwPD experience in the moment of the diagnosis announcement were related with male gender [OR = 2.034, CI 95% 1.09–3.78; p = 0.025] and older age [OR = 1.05, CI 95% 1.01–1.08; p = 0.004], while tremor as the first symptom had a threshold significance [OR = 1.78, CI 95% 0.994–3.187; p = 0.052]. Half of the PwPD and caregivers considered that they did not receive enough information and one third had a short-term appointment to rediscuss the diagnosis. A total of 82% of PwPD expressed the willingness to have a multidisciplinary follow-up (PD nurse, psychologists). Only 24% of the HCPs had been trained for PD announcement. Conclusions: The way a PD diagnosis is delivered represents a pivotal moment in the journey of PwPD and caregivers. This process requires improvement in addressing the gaps expressed by PwPD, caregivers, and HCPs through a participatory approach.
Bridge to Life: Current Landscape of Temporary Mechanical Circulatory Support in Heart-Failure-Related Cardiogenic Shock
Authors: Vlachakis Panayotis K. , Theofilis Panagiotis , Leontsinis Ioannis , Drakopoulou Maria , Karakasis Paschalis , Oikonomou Evangelos , Chrysohoou Christina , Tsioufis Konstantinos , Tousoulis Dimitris
Published: July 2024

Acute heart failure (HF) presents a significant mortality burden, necessitating continuous therapeutic advancements. Temporary mechanical circulatory support (MCS) is crucial in managing cardiogenic shock (CS) secondary to acute HF, serving as a bridge to recovery or durable support. Currently, MCS options include the Intra-Aortic Balloon Pump (IABP), TandemHeart (TH), Impella, and Veno-Arterial Extracorporeal Membrane Oxygenation (VA-ECMO), each offering unique benefits and risks tailored to patient-specific factors and clinical scenarios. This review examines the clinical implications of recent advancements in temporary MCS, identifies knowledge gaps, and explores promising avenues for future research and clinical application. Understanding each device’s unique attributes is crucial for their efficient implementation in various clinical scenarios, ultimately advancing towards intelligent, personalized support strategies.