bluuebunny/crossref_metadata_2025 huggingface dataset:
Towards a National Harmonized Framework for Urban Plans and Strategies in Romania
Authors: Luca Oana , Gaman Florian , Răuță Emanuel
Published: February 2021

The present paper aims to open the discussion on the adoption of a simplified, flexible, and harmonized strategic framework for city development in Romania. Besides a development strategy associated with general urban planning, multiple strategies are elaborated in accordance with the requirements of the financing authorities and the specific spatial planning legislation. These strategies were developed at different times by different institutions and aim to substantiate the directions and priorities of development and attract funding for various programs. In this study, we performed a thorough analysis in eight municipalities and concluded that such strategies cannot be effective, as they are not always coordinated and often lack consistency and complementarity with other strategic and operational plans and programs at the local level. Based on an international literature review including the recommendations of the Harmonization for Sustainable Energy, Climate Action Plans, and Sustainable Urban Mobility Plans developed by the EU financed project “Sustainable Integrated Multi-sector Planning” (SIMPLA), we propose a unification of the various strategies in a single harmonized, flexible strategic structure for the city. This harmonized structure includes specific components related to resilience in case of disasters and financing sources for all proposed projects.
Exploring the Sustainability of the Intermediary Role in Blockchain
Authors: Tseng Cheng-Te , Shang Shari S. C.
Published: February 2021

Traditional business models involve cost, market development, distribution channels, business partnerships, and supply chain management. The development of the digital economy and digital network technology has engendered a shift away from the traditional model of operation. Intermediaries have long played an essential role in promoting the benefits of economic activities, but new technology is increasingly replacing intermediaries in their roles of connecting players, such as, involving, committing, and mobilizing players. Potential conflicts of interest must therefore be further resolved, avoided, or mitigated. Blockchain technology, as a tool for keeping immutable and digital records, can address increasingly complex issues in global value chains to pursue sustainable development. It attempts to realize the trust mechanism and has been redefining the function of intermediaries. This study used a multiple-case study approach to examine how blockchain technology affects intermediate functionality. We evaluated the industry’s use of blockchains to assess how the processes were reshaped and how the intermediary roles were refined. On the basis of the findings, we propose three potential changes for the roles of intermediaries to improve operational efficiency.
Sustainability as a “New Normal” for Modern Businesses: Are SMEs of Pakistan Ready to Adopt It?
Authors: Ahmad Naveed , Mahmood Asif , Han Heesup , Ariza-Montes Antonio , Vega-Muñoz Alejandro , Din Mohi ud , Iqbal Khan Ghazanfar , Ullah Zia
Published: February 2021

While in developed countries, the concept of sustainability is being recognized as a “new normal” for modern businesses, the situation is entirely different in the context of developing and underdeveloped regions of the world. Shockingly, most of the developing nations do not consider the environmental dimension of CSR as their priority, and this is one of the reasons that the issue of climate change is a hot topic of debate among contemporary researchers and policymakers from developing economies. The present study aims to explore the type and level of CSR engagement among different organizations of the SME sector in Pakistan. Further, the study also intends to uncover any potential difference between medium-sized enterprises and small-sized enterprises in terms of their CSR preferences and engagements. The study uses a qualitative approach in order to get deeper insights to meet study objectives through in-depth semi-structured interviews. In this regard, the data were collected from nine SMEs belonging to different sectors in Lahore city of Pakistan. The authors used thematic analysis in order to analyze in-depth interviews, and three main themes were extracted, including social CSR, economic CSR, and environmental CSR. The results revealed that most of the medium-sized enterprises prefer the social dimension of CSR, whereas small-sized enterprises are largely concerned with the economic dimension of CSR. It has also been revealed that the environmental dimension of CSR is non-existent in the case of small-sized enterprises. The results of the present study will help policymakers to better understand the phenomenon of CSR and to rethink their CSR policies for a better and sustainable future.
The Impact of Water-Related Pollution on Food Systems in Transition: The Case of Northern Vietnam
Authors: Linderhof Vincent , Meeske Marieke , Diogo Vasco , Sonneveld Anne
Published: February 2021

In Vietnam, agricultural practices such as fertilizer and pesticide use affect the landscape as well as the availability and safety of food. For instance, pesticides and fertilizer end up in surface water used for drinking water, crop irrigation, and in fish tanks. However, the link to actual food consumption and health is complex and information is lacking. This study considers potential water-related exposure to toxic hazards in northern Vietnam food systems, through the consumption of food commodities and of water. Water pollution is operationalized by considering the following two channels: (i) pesticide and nutrient leaching to surface water (share of surface water) and (ii) industrial runoff from facilities located in urban areas (share of urban areas). We explore how potential exposure to toxic hazard is related to food consumption choices. Using a sample of the Vietnam Household Living Standard Survey (VHLSS) for 2014, we estimate how shares of food consumption categories in total food consumption are affected by household and landscape characteristics, the latter also reflecting potential environmental pressures. In districts with higher shares of surface water, the share of fish consumption is higher and the share of meat consumption is lower. From an environmental and health perspective, households in water-rich areas thus may have a higher probability of being exposed to toxic chemicals due to higher fish consumption. In districts with higher shares of urban areas, the shares of meat and cereals in total food consumption value were lower, and the shares of fish and fruit and vegetables were higher. The results indicate that food consumption is affected by landscape characteristics that may also influence the level of exposure to water-related environmental pressures, and that this combined effect may potentially exacerbate food safety and health risks. The actual impact is more complex and should be analyzed with more sophisticated data and methods.
Synopsis of Factors Affecting Hydrogen Storage in Biomass-Derived Activated Carbons
Authors: Sultana Al Ibtida , Saha Nepu , Reza M. Toufiq
Published: February 2021

Hydrogen (H2) is largely regarded as a potential cost-efficient clean fuel primarily due to its beneficial properties, such as its high energy content and sustainability. With the rising demand for H2 in the past decades and its favorable characteristics as an energy carrier, the escalating USA consumption of pure H2 can be projected to reach 63 million tons by 2050. Despite the tremendous potential of H2 generation and its widespread application, transportation and storage of H2 have remained the major challenges of a sustainable H2 economy. Various efforts have been undertaken by storing H2 in activated carbons, metal organic frameworks (MOFs), covalent organic frameworks (COFs), etc. Recently, the literature has been stressing the need to develop biomass-based activated carbons as an effective H2 storage material, as these are inexpensive adsorbents with tunable chemical, mechanical, and morphological properties. This article reviews the current research trends and perspectives on the role of various properties of biomass-based activated carbons on its H2 uptake capacity. The critical aspects of the governing factors of H2 storage, namely, the surface morphology (specific surface area, pore volume, and pore size distribution), surface functionality (heteroatom and functional groups), physical condition of H2 storage (temperature and pressure), and thermodynamic properties (heat of adsorption and desorption), are discussed. A comprehensive survey of the literature showed that an “ideal” biomass-based activated carbon sorbent with a micropore size typically below 10 Å, micropore volume greater than 1.5 cm3/g, and high surface area of 4000 m2/g or more may help in substantial gravimetric H2 uptake of >10 wt% at cryogenic conditions (−196 °C), as smaller pores benefit by stronger physisorption due to the high heat of adsorption.
Alkaline Fractionation and Subsequent Production of Nano-Structured Silica and Cellulose Nano-Fibrils for the Comprehensive Utilization of Rice Husk
Authors: Jung Hyun , Kwak Hyun , Chun Jinyoung , Oh Kyeong
Published: February 2021

The parameters of the alkaline fractionation process were investigated and optimized using a statistical analysis method to simultaneously remove hemicellulose and ash from rice husk (RH) concomitantly. After the alkaline fractionation process, the residual solid contained high cellulose, and the recovery yield of hemicellulose was enhanced in the fractionated liquid hydrolyzate. The hemicellulosic sugar recovery yield (71.6%), de-ashing yield (>99%), and lignin removal (>80%) were obtained at the reaction conditions of 150 °C of temperature, 40 min of reaction time, and 6% (w/v) of NaOH concentration. Subsequently, nano-structured silica was synthesized using black liquor obtained as a by-product of this fractionation process. For the production of nano-structured silica, it was observed that the pH of a black liquor and the heat treatment temperature significantly influenced the textural properties of silica product. In addition, the two-stage bleaching of solid residue followed by colloid milling for the production of high value-added CNF with was attempted. As a result, in addition to 119 g of fermentable sugar, 143 g of high-purity (>98%) silica with a surface area of 328 m2g−1 and 273.1 g of high-functional CNF with cellulose content of 80.1% were simultaneously obtained from 1000 g of RH.
Trade Openness and CO2 Emissions: The Heterogeneous and Mediating Effects for the Belt and Road Countries
Authors: Chen Fuzhong , Jiang Guohai , Kitila Getachew Magnar
Published: February 2021

To investigate whether increasing trade openness results in more severe environmental problems, this study investigates the impact of trade openness on carbon dioxide (CO2) emissions using panel data from 64 countries along the Belt and Road from 2001–2019. Fully considering the potential heterogeneity, the panel quantile regression approach is utilized. Moreover, this study explores the three major mediating effects of the process, namely the energy-substitution effect, economic effect, and technology effect. The empirical results indicate that the improvement in trade openness has a significantly positive effect on CO2 emissions, and it also shows that the impact varies with different levels of CO2 emissions. Furthermore, the indirect effect of trade openness on CO2 emissions via the economic effect is positive, while the indirect effect via the energy-substitution and the technology effect is negative. Therefore, it is necessary to improve renewable energy consumption, decrease energy intensity, and formulate related policies to reduce carbon emissions policies in terms of local conditions.
Agricultural Innovation and Sustainable Development: A Case Study of Rice–Wheat Cropping Systems in South Asia
Authors: Ullah Aman , Nawaz Ahmad , Farooq Muhammad , Siddique Kadambot H. M.
Published: February 2021

The rice–wheat cropping system is the main food bowl in Asia, feeding billions across the globe. However, the productivity and long-term sustainability of this system are threatened by stagnant crop yields and greenhouse gas emissions from flooded rice production. The negative environmental consequences of excessive nitrogen fertilizer use are further exacerbating the situation, along with the high labor and water requirements of transplanted rice. Residue burning in rice has also severe environmental concerns. Under these circumstances, many farmers in South Asia have shifted from transplanted rice to direct-seeded rice and reported water and labor savings and reduced methane emissions. There is a need for opting the precision agriculture techniques for the sustainable management of nutrients. Allelopathic crops could be useful in the rotation for weed management, the major yield-reducing factor in direct-seeded rice. Legume incorporation might be a viable option for improving soil health. As governments in South Asia have imposed a strict ban on the burning of rice residues, the use of rice-specific harvesters might be a pragmatic option to manage rice residues with yield and premium advantage. However, the soil/climatic conditions and farmer socio-economic conditions must be considered while promoting these technologies in rice-wheat system in South Asia.
Cross-Validation of the MEDEAS Energy-Economy-Environment Model with the Integrated MARKAL-EFOM System (TIMES) and the Long-Range Energy Alternatives Planning System (LEAP)
Authors: Perissi Ilaria , Martelloni Gianluca , Bardi Ugo , Natalini Davide , Jones Aled , Nikolaev Angel , Eggler Lukas , Baumann Martin , Samsó Roger , Solé Jordi
Published: February 2021

In the present study, we compare energy transition scenarios from a new set of integrated assessment models, the suite of MEDEAS models, based on a systems dynamic modeling approach, with scenarios from two already well know structurally and conceptually different integrated assessment models, the Integrated MARKAL-EFOM System (TIMES) and the Long-Range Energy Alternatives Planning system (LEAP). The investigation was carried out to cross-compare and benchmark the response of MEDEAS models with TIMES and LEAP in depicting the energy transition in two different countries, Austria and Bulgaria. The preliminary results show a good agreement across all the models in representing scenarios projecting historical trends, while a major discrepancy is detectable when the rate of implementation of renewable energy is forced to increase to achieve energy system decarbonization. The discrepancy is mainly traceable to the differences in the models’ conception and structures rather than in a real mismatch in representing the same scenarios. The present study is put forward as a guideline for validating new modeling approaches that link energy policy decision tools to the global biophysical and socioeconomic constraints.
Rural Economic Development Based on Shift-Share Analysis in a Developing Country: A Case Study in Heilongjiang Province, China
Authors: Lv Donghui , Gao Huiying , Zhang Yu
Published: February 2021

Identification of local priorities within each potential sector and implementation of a targeted development policy would definitely accelerate rural economic growth. In this sense, it is useful to examine each region’s industrial structural evolution compared to the whole economy and aggregate industries. Shift-share analysis has been confirmed as a useful method to measure regional economic differences and analyze the contribution of industrial structure. This paper selects five representative counties in Heilongjiang province and applies shift-share decomposition to analyze the change in rural economic development from 2000 to 2018. The change of economic growth in each selected county is decomposed into three components: national growth effect, industrial structure effect, and competitive effect, taking the national level as the reference. The results showed the following: (1) the trend of rural economic growth fluctuated greatly for nearly 20 years, distinguished by a mismatch of industrial structure with competitiveness for the selected counties; rural economies with an inappropriate industrial structure did not experience strong growth, despite high competitive potential. (2) The low-end agricultural structure and secondary industry structure led to the loss of each competitive effect; the tertiary industry structure based on economic structure servitization was rational, but the competitive effect did not work out. (3) Finally, this paper provided differentiated suggestions in accordance with local resources and priorities of the selected counties, so as to avoid excessive convergence and the lack of characteristics in industrial structure in rural transformation.
Catalyzing Innovation: Governance Enablers of Nature-Based Solutions
Authors: Martin Juliette G. C. , Scolobig Anna , Linnerooth-Bayer JoAnne , Liu Wei , Balsiger Jörg
Published: February 2021

There is growing recognition that using the properties of nature through nature-based solutions (NBS) can help to provide viable and cost-effective solutions to a wide range of societal challenges, including disaster risk reduction (DRR). However, NBS realization depends critically on the governance framework that enables the NBS policy process. Drawing from three case studies in Nocera Inferiore (Italy), Munich (Germany), and Wolong (China), we identify key governance enablers—the contextual preconditions, policy processes, and institutions—that proved essential for NBS initiation, planning, design, and implementation. In the three cases, interviews confirm the success of the NBS measures and their benefits in terms not only of DRR but of multiple ecological and social–economic co-benefits. Results highlight critical governance enablers of NBS, including: polycentric governance (novel arrangements in the public administration that involved multiple institutional scales and/or sectors); co-design (innovative stakeholder participatory processes that influenced the final NBS); pro-NBS interest and coalition groups (organized pressure groups that advocated for an NBS); and financial incentives (financing community-based implementation and monitoring of NBS). Findings show that the transition to NBS can contribute to multiple global agendas, including DRR, climate change adaptation, and sustainable development.
Does Social Businesses Development Affect Bioenergy Industry Growth under the Pathway of Sustainable Development?
Authors: Alsaleh Mohd , Abdulwakil Muhammad , Abdul-Rahim Abdul
Published: February 2021

The Treaty of European Union (EU) sets out the EU vision for sustainable development of Europe based on balanced economic growth and price stability, a highly competitive social market economy, aiming at full employment and social progress, and a high level of protection and improvement of the quality of the environment. This led us to ask whether or not social business development stimulates the development of the bioenergy sector in the EU28 countries. Given the increasing rates of energy insecurity, environmental pollution, poverty, and unemployment, countries are switching to alternative energy sources that might promote social business development, climate change, and environmental quality. In this scenario, the bioenergy industry has received the attention of scholars and policymakers alike. The role social business development can play in the growth of the bioenergy industry remains uncertain, therefore, further investigation is necessary. This study, therefore, explores the relationships between the bioenergy industry and social business development indicators related to zero emissions, zero poverty, and zero unemployment for EU28 region countries from 1990 to 2018. Empirical evidence is based on the use of a new economic model, dynamic panel co-integration simulations (Fully Modified Ordinary Least Square, Dynamic Ordinary Least Square, and Pooled Ordinary Least Square). The results reveal a negative relationship between EU28′s bioenergy industry growth and carbon dioxide emissions, vulnerable employment, and unemployment rate, suggesting that bioenergy industry growth helps reduce pollution and unemployment. Likewise, bioenergy industry growth increases food supply, economic growth, and female employment and might be the best alternative to fossil fuels. Necessary policy related to bioenergy industry growth can be formulated, especially in achieving the sustainable development goals for social businesses.
Analyzing the Impacts of Financial Services Regulation to Make the Case That Buy-Now-Pay-Later Regulation Is Failing
Authors: Johnson Di , Rodwell John , Hendry Thomas
Published: February 2021

Fee-based Buy-Now-Pay-Later services (BNPL) are becoming widely adopted in many developed countries, including Australia. Across a variety of regulatory approaches there appears to be relatively minimal regulatory coverage of fee-based BNPL. This review applies a results-oriented, behaviourally informed market failure approach to assess the regulatory outcomes of fee-based BNPL. The review makes the case that the impacts of the regulation of fee-based BNPL in Australia demonstrate multiple forms of regulatory failure. The regulatory failure is particularly due to regulatory capture at a broad level and especially in terms of a lack of consumer protections. Consumers may particularly need consideration and protection because understanding the increasing complexity and financial knowledge at the heart of many fintech services is beyond the capability or responsibility of the consumer. Incorporating social and consumer considerations into analyses of regulatory structures can enable analyses of the regulation of fintech and move financial services regulation toward providing more socially useful and sustainable financial services. In the future, a behaviourally informed approach to the regulation of fintech may be beneficial and enhance sustainability.
Eyes on the Goal! Exploring Interactive Artistic Real-Time Energy Interfaces for Target-Specific Actions in the Built Environment
Authors: Goubran Sherif , Cucuzzella Carmela , Ouf Mohamed M.
Published: February 2021

Current research is focused on sensing and modeling occupant behavior to predict it and automate building controls. Another line of research recommends influencing the behavior of occupants through feedback mechanisms and engagement. Yet, most of the work has focused on pushing occupants to reduce energy consumption over a long time and does not explore the potential to guide users to take specific actions promptly. The study examines a new interface mechanism that aims to solicit immediate and predefined actions from occupants. Building on seminal research in the field, the study uses art visualization to reinterpret social feedback. We test this approach in an immersive interaction space where participants react to artistic visuals to attain predefined settings for three indoor devices. In the 197 interactions recorded, participants’ overall actions conformed with the predefined goals. The participants were able to reach all or some of the targets in more than 80%, within an average of less than 30 seconds. We also see that complementing the visuals with textual hints improved the interaction in terms of engagement and accuracy. We conclude that ambient, abstract, and artistic real-time goal-driven feedback is effective in influencing immediate actions. We recommend that guiding occupants didactically has a strong potential for advancing building controls.
Games on Climate Change: Identifying Development Potentials through Advanced Classification and Game Characteristics Mapping
Authors: Gerber Andreas , Ulrich Markus , Wäger Flurin X. , Roca-Puigròs Marta , Gonçalves João S. V. , Wäger Patrick
Published: February 2021

The challenges posed to humanity by climate change require innovative approaches. Well-designed games are powerful tools with the potential to support solving climate related challenges. In this article, we present a mapping review study of games that address climate change issues (climate games). In a search and selection process, we identified 115 climate games that were classified by applying a newly developed game typology. This allowed gaining an overview of existing climate games and identifying potentials for future game development. The game classification revealed that the majority of climate games (75%) were designed for learning purposes and addressed heterogeneous target audiences such as students, professionals, or the “general public”. The identified games covered a wide range of characteristics, e.g., regarding the topic addressed, player interaction, game-play, level of abstraction, or the media used for play. Nevertheless, we identified areas where only few or no games were found. These “gaps” provide opportunities where future games could help solving climate challenges and include, amongst others, games designed for professionals to address specific needs and games that create a direct impact outside the game environment. Regarding game design, experimenting with the abstraction of games is currently underexplored and offers potential for future developments.
About the Cauchy–Bunyakovsky–Schwarz Inequality for Hilbert Space Operators
Authors: Minculete Nicuşor
Published: February 2021

The symmetric shape of some inequalities between two sequences of real numbers generates inequalities of the same shape in operator theory. In this paper, we study a new refinement of the Cauchy–Bunyakovsky–Schwarz inequality for Euclidean spaces and several inequalities for two bounded linear operators on a Hilbert space, where we mention Bohr’s inequality and Bergström’s inequality for operators. We present an inequality of the Cauchy–Bunyakovsky–Schwarz type for bounded linear operators, by the technique of the monotony of a sequence. We also prove a refinement of the Aczél inequality for bounded linear operators on a Hilbert space. Finally, we present several applications of some identities for Hermitian operators.
A Quantum Time Coordinate
Authors: Martínez-Pérez Armando , Torres-Vega Gabino
Published: February 2021

We discuss quantum time states formed with a finite number of energy eigenstates with the purpose of obtaining a time coordinate. These time states are eigenstates of the recently introduced discrete time operator. The coordinate and momentum representations of these time eigenstates resemble classical time curves and become classical at high energies. To illustrate this behavior, we consider the simple example of the particle-in-a-box model. We can follow the quantum-classical transition of the system. Among the many existing solutions for the particle in a box, we use a set which leads to time eigenstates for use as a coordinate system.
Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava Starch
Authors: Sánchez-Chica Jennifer , Correa Margarita M. , Aceves-Diez Angel E. , Castañeda-Sandoval Laura M.
Published: February 2021

Bacillus cereus is a human pathogenic bacterium found in foods with the potential to cause emesis and diarrhea. This study estimated the presence, toxigenic and genomic diversity of B. cereus s.l. obtained from cassava starch samples collected in bakeries and powdered food companies in Medellín (Colombia). Bacillus cereuss.l. was found in 43 of 75 (57%) cassava starch samples and 98 isolates were obtained. The nheABC, hblCDAB, cytK2, entFM and cesB toxin genes were detected by multiplex PCR and the most frequent operon was nheABC, whereas cesB gene was not found. Twelve toxigenic profiles were determined by the detection of toxin genes, and the most frequent profiles harbored all enterotoxin genes. A broad genomic diversity was detected according to GTG5-PCR fingerprinting results with 76 B. cereus s.l. grouped in sixteen clusters and the 22 isolates clustering separately. No relationship was observed between genomic background and toxigenic profiles. In general, the results showed a high genomic and enterotoxigenic diversity in B. cereus s.l. found in cassava starch. These results should incentive future studies to understand the distribution of B. cereus s.l. isolated on raw materials in comparison with finished products.
Effect of Temperature during Drying and Storage of Dried Figs on Growth, Gene Expression and Aflatoxin Production
Authors: Galván Ana Isabel , Rodríguez Alicia , Martín Alberto , Serradilla Manuel Joaquín , Martínez-Dorado Ana , Córdoba María de Guía
Published: February 2021

Dried fig is susceptible to infection by Aspergillus flavus, the major producer of the carcinogenic mycotoxins. This fruit may be contaminated by the fungus throughout the entire chain production, especially during natural sun-drying, post-harvest, industrial processing, storage, and fruit retailing. Correct management of such critical stages is necessary to prevent mould growth and mycotoxin accumulation, with temperature being one of the main factors associated with these problems. The effect of different temperatures (5, 16, 25, 30, and 37 °C) related to dried-fig processing on growth, one of the regulatory genes of aflatoxin pathway (aflR) and mycotoxin production by A. flavus, was assessed. Firstly, growth and aflatoxin production of 11 A. flavus strains were checked before selecting two strains (M30 and M144) for in-depth studies. Findings showed that there were enormous differences in aflatoxin amounts and related-gene expression between the two selected strains. Based on the results, mild temperatures, and changes in temperature during drying and storage of dried figs should be avoided. Drying should be conducted at temperatures >30 °C and close to 37 °C, while industry processing, storage, and retailing of dried figs are advisable to perform at refrigeration temperatures (<10 °C) to avoid mycotoxin production.
Nudivirus Sequences Identified from the Southern and Western Corn Rootworms (Coleoptera: Chrysomelidae)
Authors: Liu Sijun , Sappington Thomas W. , Coates Brad S. , Bonning Bryony C.
Published: February 2021

Analysis of pooled genomic short read sequence data revealed the presence of nudivirus-derived sequences from U.S. populations of both southern corn rootworm (SCR, Diabrotica undecimpunctata howardi Barber) and western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte). A near complete nudivirus genome sequence was assembled from sequence data for an SCR population with relatively high viral titers. A total of 147,179 bp was assembled from five contigs that collectively encode 109 putative open reading frames (ORFs) including 20 nudivirus core genes. In contrast, genome sequence recovery was incomplete for a second nudivirus from WCR, although sequences derived from this virus were present in three geographically dispersed populations. Only 48,989 bp were assembled with 48 putative ORFs including 13 core genes, representing about 20% of a typical nudivirus genome. Phylogenetic analysis indicated that both corn rootworm nudiviruses grouped with the third known nudivirus of beetles, Oryctes rhinoceros nudivirus in the genus Alphanudivirus. On the basis of phylogenetic and additional analyses, we propose further taxonomic separation of nudiviruses within Alphanudivirus and Betanudivirus into two subfamilies and five genera. Identification of nudivirus-derived sequences from two species of corn rootworm highlights the diversity of viruses associated with these agricultural insect pests.
A Canine Distemper Virus Retrospective Study Conducted from 2011 to 2019 in Central Italy (Latium and Tuscany Regions)
Authors: Ricci Ida , Cersini Antonella , Manna Giuseppe , Marcario Gaetana Anita , Conti Raffaella , Brocherel Giuseppina , Grifoni Goffredo , Eleni Claudia , Scicluna Maria Teresa
Published: February 2021

Canine distemper virus (CDV) is a highly lethal contagious viral pathogen mainly found in domestic and wild canids and mustelids. Although, in Italy, circulating strains of Europe 1, Europe wildlife and Arctic type are reported, data relating to Latium and Tuscany regions are limited. In view of this, through passive surveillance, we investigated the presence of CDV and which strains were circulating in these Regions. From March 2017 to October 2019, a group of 122 subjects were tested for CDV using a PCR protocol described in the literature, with 12 detected positive; analyses were carried out on a set of target samples (brain and lung, conjunctival, nasal and rectal swabs, urine or swab from bladder and intracardiac clot) that was defined for the detection of CDV in both live and dead animals. The rectal swab, easily collected also from live animals, represented the most suitable sample for CDV diagnosis, with 9 positive of the 11 (81.82%) tested. In addition, brain and lung of 15 subjects out of 181 susceptible animals collected between 2011 and 2018, during post mortem investigations in routine diagnostic activity, were CDV positive. Molecular analyses of all positive samples, using a 287 bp fragment located within the conserved N terminus of the morbillivirus nucleoprotein gene, detected the circulation of strain CDV599/2016 (KX545421.1) belonging to the “Europe wildlife” lineage, and of strain CDV12254/2015 (KX024709.1), belonging to the Arctic-lineage, thus confirming the co-circulation of the two lineages, as already noted in previous studies.
The HIV-1 Capsid: From Structural Component to Key Factor for Host Nuclear Invasion
Authors: Scoca Viviana , Di Nunzio Francesca
Published: February 2021

Since the discovery of HIV-1, the viral capsid has been recognized to have an important role as a structural protein that holds the viral genome, together with viral proteins essential for viral life cycle, such as the reverse transcriptase (RT) and the integrase (IN). The reverse transcription process takes place between the cytoplasm and the nucleus of the host cell, thus the Reverse Transcription Complexes (RTCs)/Pre-integration Complexes (PICs) are hosted in intact or partial cores. Early biochemical assays failed to identify the viral CA associated to the RTC/PIC, possibly due to the stringent detergent conditions used to fractionate the cells or to isolate the viral complexes. More recently, it has been observed that some host partners of capsid, such as Nup153 and CPSF6, can only bind multimeric CA proteins organized in hexamers. Those host factors are mainly located in the nuclear compartment, suggesting the entrance of the viral CA as multimeric structure inside the nucleus. Recent data show CA complexes within the nucleus having a different morphology from the cytoplasmic ones, clearly highlighting the remodeling of the viral cores during nuclear translocation. Thus, the multimeric CA complexes lead the viral genome into the host nuclear compartment, piloting the intranuclear journey of HIV-1 in order to successfully replicate. The aim of this review is to discuss and analyze the main discoveries to date that uncover the viral capsid as a key player in the reverse transcription and PIC maturation until the viral DNA integration into the host genome.
The Paradoxes of Viral mRNA Translation during Mammalian Orthoreovirus Infection
Authors: Guo Yingying , Parker John S. L.
Published: February 2021

De novo viral protein synthesis following entry into host cells is essential for viral replication. As a consequence, viruses have evolved mechanisms to engage the host translational machinery while at the same time avoiding or counteracting host defenses that act to repress translation. Mammalian orthoreoviruses are dsRNA-containing viruses whose mRNAs were used as models for early investigations into the mechanisms that underpin the recognition and engagement of eukaryotic mRNAs by host cell ribosomes. However, there remain many unanswered questions and paradoxes regarding translation of reoviral mRNAs in the context of infection. This review summarizes the current state of knowledge about reovirus translation, identifies key unanswered questions, and proposes possible pathways toward a better understanding of reovirus translation.
VOC 202012/01 Variant Is Effectively Neutralized by Antibodies Produced by Patients Infected before Its Diffusion in Italy
Authors: Rondinone Valeria , Pace Lorenzo , Fasanella Antonio , Manzulli Viviana , Parisi Antonio , Capobianchi Maria Rosaria , Ostuni Angelo , Chironna Maria , Caprioli Elisabetta , Labonia Maria , Cipolletta Dora , Della Rovere Ines , Serrecchia Luigina , Petruzzi Fiorenza , Pennuzzi Germana , Galante Domenico
Published: February 2021

The coronavirus disease 2019 (Covid-19) pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and presents a global health emergency that needs urgent intervention. Viruses constantly change through mutation, and new variants of a virus are expected to occur over time. In the United Kingdom (UK), a new variant called B.1.1.7 has emerged with an unusually large number of mutations. The aim of this study is to evaluate the level of protection of sera from 12 patients infected and later healed in Apulia Region (Italy) with Covid-19 between March and November 2020, when the English variant was not circulating in this territory yet, against the new VOC 202012/01 variant by seroneutralization assay. The sera of patients had already been tested before, using a virus belonging to the lineage B.1 and showed an antibody neutralizing titer ranging between 1:160 and 1:320. All the 12 sera donors confirmed the same titers of neutralizing antibodies obtained with a strain belonging to the lineage B.1.1.7 (VOC 202012/01). These data indicate that antibodies produced in subjects infected with variants of Sars-CoV-2 strain before the appearance of the English one, seem to have a neutralizing power also against this variant.
Diagnostic SARS-CoV-2 Cycle Threshold Value Predicts Disease Severity, Survival, and Six-Month Sequelae in COVID-19 Symptomatic Patients
Authors: Trunfio Mattia , Venuti Francesco , Alladio Francesca , Longo Bianca Maria , Burdino Elisa , Cerutti Francesco , Ghisetti Valeria , Bertucci Roberto , Picco Carlo , Bonora Stefano , Di Perri Giovanni , Calcagno Andrea
Published: February 2021

To date, there is no severe acute respiratory syndrome coronavirus 2-(SARS-CoV-2)-specific prognostic biomarker available. We assessed whether SARS-CoV-2 cycle threshold (Ct) value at diagnosis could predict novel CoronaVirus Disease 2019 (COVID-19) severity, clinical manifestations, and six-month sequelae. Hospitalized and outpatient cases were randomly sampled from the diagnoses of March 2020 and data collected at 6 months by interview and from the regional database for COVID-19 emergency. Patients were stratified according to their RNA-dependent-RNA-polymerase Ct in the nasopharyngeal swab at diagnosis as follows: Group A ≤ 20.0, 20.0 < group B ≤ 28.0, and Group C > 28.0. Disease severity was classified according to a composite scale evaluating hospital admission, worst oxygen support required, and survival. Two hundred patients were included, 27.5% in Groups A and B both, 45.0% in Group C; 90% of patients were symptomatic and 63.7% were hospitalized. The median time from COVID-19 onset to swab collection was five days. Lethality, disease severity, type, and number of signs and symptoms, as well as six-month sequelae distributed inversely among the groups with respect to SARS-CoV-2 Ct. After controlling for confounding, SARS-CoV-2 Ct at diagnosis was still associated with COVID-19-related death (p = 0.023), disease severity (p = 0.023), number of signs and symptoms (p < 0.01), and presence of six-month sequelae (p < 0.01). Early quantification of SARS-CoV-2 may be a useful predictive marker to inform differential strategies of clinical management and resource allocation.
Small-Angle X-ray Scattering Models of APOBEC3B Catalytic Domain in a Complex with a Single-Stranded DNA Inhibitor
Authors: Barzak Fareeda M. , Ryan Timothy M. , Kvach Maksim V. , Kurup Harikrishnan M. , Aihara Hideki , Harris Reuben S. , Filichev Vyacheslav V. , Harjes Elena , Jameson Geoffrey B.
Published: February 2021

In normal cells APOBEC3 (A3A-A3H) enzymes as part of the innate immune system deaminate cytosine to uracil on single-stranded DNA (ssDNA) to scramble DNA in order to give protection against a range of exogenous retroviruses, DNA-based parasites, and endogenous retroelements. However, some viruses and cancer cells use these enzymes, especially A3A and A3B, to escape the adaptive immune response and thereby lead to the evolution of drug resistance. We have synthesized first-in-class inhibitors featuring modified ssDNA. We present models based on small-angle X-ray scattering (SAXS) data that (1) confirm that the mode of binding of inhibitor to an active A3B C-terminal domain construct in the solution state is the same as the mode of binding substrate to inactive mutants of A3A and A3B revealed in X-ray crystal structures and (2) give insight into the disulfide-linked inactive dimer formed under the oxidizing conditions of purification.
Development of a Viral-Like Particle Candidate Vaccine Against Novel Variant Infectious Bursal Disease Virus
Authors: Wang Yulong , Jiang Nan , Fan Linjin , Gao Li , Li Kai , Gao Yulong , Niu Xinxin , Zhang Wenying , Cui Hongyu , Liu Aijing , Pan Qing , Liu Changjun , Zhang Yanping , Wang Xiaomei , Qi Xiaole
Published: February 2021

Infectious bursal disease (IBD), an immunosuppressive disease of young chickens, is caused by infectious bursal disease virus (IBDV). Novel variant IBDV (nVarIBDV), a virus that can evade immune protection against very virulent IBDV (vvIBDV), is becoming a threat to the poultry industry. Therefore, nVarIBDV-specific vaccine is much needed for nVarIBDV control. In this study, the VP2 protein of SHG19 (a representative strain of nVarIBDV) was successfully expressed using an Escherichia coli expression system and further purified via ammonium sulfate precipitation and size-exclusion chromatography. The purified protein SHG19-VP2-466 could self-assemble into 25-nm virus-like particle (VLP). Subsequently, the immunogenicity and protective effect of the SHG19-VLP vaccine were evaluated using animal experiments, which indicated that the SHG19-VLP vaccine elicited neutralization antibodies and provided 100% protection against the nVarIBDV. Furthermore, the protective efficacy of the SHG19-VLP vaccine against the vvIBDV was evaluated. Although the SHG19-VLP vaccine induced a comparatively lower vvIBDV-specific neutralization antibody titer, it provided good protection against the lethal vvIBDV. In summary, the SHG19-VLP candidate vaccine could provide complete immune protection against the homologous nVarIBDV as well as the heterologous vvIBDV. This study is of significance to the comprehensive prevention and control of the recent atypical IBD epidemic.
Entry and Disposition of Zika Virus Immune Complexes in a Tissue Culture Model of the Maternal-Fetal Interface
Authors: Xu Yanqun , He Yong , Momben-Abolfath Sanaz , Eller Nancy , Norton Malgorzata , Zhang Pei , Scott Dorothy , Struble Evi Budo
Published: February 2021

Zika virus (ZIKV) infections have been associated with an increased incidence of severe microcephaly and other neurodevelopmental disorders in newborn babies. Passive immunization with anti-ZIKV neutralizing antibodies has the potential to become a feasible treatment or prophylaxis option during pregnancy. Prior to clinical use, such antibodies should be assessed for their ability to block ZIKV passage to the fetus. We used human placental and mammalian cell monolayers that express FcRn and laboratory preparations of anti-ZIKV antibodies as a model system to investigate the disposition of ZIKV/antibody immune complexes (ICs) at the maternal-fetal interface. We further characterized solution properties of the ICs to evaluate whether these are related to in vitro effects. We found that both ZIKV and ZIKV envelope glycoprotein can enter and passage through epithelial cells, especially those that overexpress FcRn. In the presence of ZIKV antibodies, Zika virus entry was bimodal, with reduced entry at the lowest (0.3–3 ng/mL) and highest (µg/mL) antibody concentrations. Intermediate concentrations attenuated inhibition or enhanced viral entry. With respect to anti-ZIKV antibodies, we found that their degradation was accelerated when presented as ICs containing increased amounts of ZIKV immunogen. Of the two monoclonal antibodies tested, the preparation with higher aggregation also exhibited higher degradation. Our studies confirm that intact Zika virus and its envelope immunogen have the potential to enter and be transferred across placental and other epithelial cells that express FcRn. Presence of anti-ZIKV IgG antibodies can either block or enhance cellular entry, with the antibody concentration playing a complex role in this process. Physicochemical properties of IgG antibodies can influence their degradation in vitro.
Unified Packing Model for Improved Prediction of Porosity and Hydraulic Conductivity of Binary Mixed Soils
Authors: El-Husseiny Ammar
Published: February 2021

Binary mixed soils, containing coarse sand particles mixed with variable content of fines (fine sand, silt, or clay) are important for several environmental and engineering applications. The packing state (or porosity) of such sand-fines mixtures controls several important physical properties such as hydraulic conductivity. Therefore, developing an analytical packing model to predict porosity of binary mixed soils, based on properties of pure unmixed sand and fines (endmembers), can contribute to predicting hydraulic conductivity for the mixtures without the need for extensive laboratory measurements. Toward this goal, this study presents a unified packing model for the purpose of predicting the porosity and hydraulic conductivity of binary mixed soils as function of fines fraction. The current model modifies an existing packing model developed for coarse binary mixed soils to achieve three main improvements: (1) being inclusive of wide range of binary mixed soils covering the whole range particle sizes, (2) incorporating the impact of cohesive packing behavior of the fines on binary mixture porosity, and (3) accounting for the impact of clay swelling. The presented model is the first of its kind incorporating the combined impact of all three factors: particle size ratio, fines cohesive packing and swelling, on binary mixtures porosity. The predictions of the modified model are validated using experimental published data for the porosity of sand-fines mixtures from 24 different studies. The model shows significant improvement in predicting porosity compared to existing packing models that frequently underestimate the porosity. By using the predicted porosity as an input in Kozeny–Carman formulation, the absolute mean error in predicting hydraulic conductivity, as function of fines fraction for 16 different binary mixed soils, is reduced by 50% when compared to the use of the previous packing model. The current model provides insights about the endmembers properties (porosity, hydraulic conductivity, and grain size) and fines content required to achieve a certain target desirable porosity and hydraulic conductivity of the mixed soils. This can assist the optimization of soil mixing design for various applications.