bluuebunny/crossref_metadata_2025 huggingface dataset:
“I Was Very Shocked, I Wanted It to Be Over”: A Qualitative Exploration of Suicidal Ideation and Attempts among Women Living with HIV in Indonesia
Authors: Fauk Nelsensius Klau , Asa Gregorius Abanit , McLean Caitlan , Ward Paul Russell
Published: December 2023

HIV diagnosis and poor HIV management have various detrimental impacts on the lives of people living with HIV (PLHIV). As a part of a large qualitative study investigating HIV risk factors and impacts, of which the topic of suicide is not a focus, this paper describes the factors contributing to suicidal ideation and attempts that arose naturally in the stories of women living with HIV (WLHIV; n = 52) in Yogyakarta and Belu districts, Indonesia. The participants were recruited using the snowball sampling technique. Guided by a qualitative data analysis framework, the data were thematically analysed. The findings were grouped into four main themes: (i) the women experienced immense psychological challenges due to the infection, spousal transmission, fear of mother-to-child transmission, and losing a child due to AIDS, which triggered suicidal ideation and attempts; (ii) the lack of awareness of HIV management strategies resulted in them feeling trapped and overwhelmed, and the associated negative thoughts and the anticipation and experience of HIV stigma influenced their thoughts of suicide; (iii) the lack of social support from family and friends during the early stages of HIV diagnosis, compounded with pre-existing financial difficulties, lack of income, unemployment, and feeling overburdened, also triggered the women’s thoughts of suicide; and (iv) family breakdown following HIV diagnosis, concern about future relationships, and fear of being rejected or abandoned by their partner were also influencing factors for suicidal ideation and attempts among the women. The findings indicate the need for a nuanced approach to counselling within HIV care interventions for couples to support the acceptance of each other’s HIV status whilst maintaining psychological wellbeing. Additionally, the findings indicate the importance of HIV education and awareness among community members for the de-stigmatisation of HIV and to increase the acceptance of PLHIV by their families and communities.
Back-Up Base Excision DNA Repair in Human Cells Deficient in the Major AP Endonuclease, APE1
Authors: Kim Daria V. , Diatlova Evgeniia A. , Zharkov Timofey D. , Melentyev Vasily S. , Yudkina Anna V. , Endutkin Anton V. , Zharkov Dmitry O.
Published: December 2023

Apurinic/apyrimidinic (AP) sites are abundant DNA lesions generated both by spontaneous base loss and as intermediates of base excision DNA repair. In human cells, they are normally repaired by an essential AP endonuclease, APE1, encoded by the APEX1 gene. Other enzymes can cleave AP sites by either hydrolysis or β-elimination in vitro, but it is not clear whether they provide the second line of defense in living cells. Here, we studied AP site repairs in APEX1 knockout derivatives of HEK293FT cells using a reporter system based on transcriptional mutagenesis in the enhanced green fluorescent protein gene. Despite an apparent lack of AP site-processing activity in vitro, the cells efficiently repaired the tetrahydrofuran AP site analog resistant to β-elimination. This ability persisted even when the second AP endonuclease homolog, APE2, was also knocked out. Moreover, APEX1 null cells were able to repair uracil, a DNA lesion that is removed via the formation of an AP site. If AP site hydrolysis was chemically blocked, the uracil repair required the presence of NTHL1, an enzyme that catalyzes β-elimination. Our results suggest that human cells possess at least two back-up AP site repair pathways, one of which is NTHL1-dependent.
A Model of the Full-Length Cytokinin Receptor: New Insights and Prospects
Authors: Arkhipov Dmitry V. , Lomin Sergey N. , Romanov Georgy A.
Published: December 2023

Cytokinins (CK) are one of the most important classes of phytohormones that regulate a wide range of processes in plants. A CK receptor, a sensor hybrid histidine kinase, was discovered more than 20 years ago, but the structural basis for its signaling is still a challenge for plant biologists. To date, only two fragments of the CK receptor structure, the sensory module and the receiver domain, were experimentally resolved. Some other regions were built up by molecular modeling based on structures of proteins homologous to CK receptors. However, in the long term, these data have proven insufficient for solving the structure of the full-sized CK receptor. The functional unit of CK receptor is the receptor dimer. In this article, a molecular structure of the dimeric form of the full-length CK receptor based on AlphaFold Multimer and ColabFold modeling is presented for the first time. Structural changes of the receptor upon interacting with phosphotransfer protein are visualized. According to mathematical simulation and available data, both types of dimeric receptor complexes with hormones, either half- or fully liganded, appear to be active in triggering signals. In addition, the prospects of using this and similar models to address remaining fundamental problems of CK signaling were outlined.
Development of a Predictive Model for Optimization of Embryo Transfer Timing Using Blood-Based microRNA Expression Profile
Authors: Chen Ming-Jer , Hsu An , Lin Pei-Yi , Chen Yu-Ling , Wu Ko-Wen , Chen Kuan-Chun , Wang Tiffany , Yi Yu-Chiao , Kung Hsiao-Fan , Chang Jui-Chun , Yang Wen-Jui , Lu Farn , Guu Hwa-Fen , Chen Ya-Fang , Chuan Shih-Ting , Chen Li-Yu , Chen Ching-Hung , Yang Pok Eric , Huang Jack Yu-Jen
Published: December 2023

MicroRNAs (miRNAs) can regulate the expression of genes involved in the establishment of the window of implantation (WOI) in the endometrium. Recent studies indicated that cell-free miRNAs in uterine fluid and blood samples could act as alternative and non-invasive sample types for endometrial receptivity analysis. In this study, we attempt to systematically evaluate whether the expression levels of cell-free microRNAs in blood samples could be used as non-invasive biomarkers for assessing endometrial receptivity status. We profiled the miRNA expression levels of 111 blood samples using next-generation sequencing to establish a predictive model for the assessment of endometrial receptivity status. This model was validated with an independent dataset (n = 73). The overall accuracy is 95.9%. Specifically, we achieved accuracies of 95.9%, 95.9%, and 100.0% for the pre-receptive group, the receptive group, and the post-respective group, respectively. Additionally, we identified a set of differentially expressed miRNAs between different endometrial receptivity statuses using the following criteria: p-value < 0.05 and fold change greater than 1.5 or less than −1.5. In conclusion, the expression levels of cell-free miRNAs in blood samples can be utilized in a non-invasive manner to distinguish different endometrial receptivity statuses.
Structural Studies of Klebsiella pneumoniae Fosfomycin-Resistance Protein and Its Application for the Development of an Optical Biosensor for Fosfomycin Determination
Authors: Varotsou Christina , Ataya Farid , Papageorgiou Anastassios C. , Labrou Nikolaos E.
Published: December 2023

Fosfomycin-resistance proteins (FosAs) are dimeric metal-dependent glutathione transferases that conjugate the antibiotic fosfomycin (Fos) to the tripeptide glutathione (γ-Glu-Cys-Gly, GSH), rendering it inactive. In the present study, we reported a comparative analysis of the functional features of two FosAs from Pseudomonas aeruginosa (FosAPA) and Klebsiella pneumoniae (FosAKP). The coding sequences of the enzymes were cloned into a T7 expression vector, and soluble active enzymes were expressed in E. coli. FosAKP displayed higher activity and was selected for further studies. The crystal structure of the dimeric FosAKP was determined via X-ray crystallography at 1.48 Å resolution. Fos and tartrate (Tar) were found bound in the active site of the first and second molecules of the dimer, respectively. The binding of Tar to the active site caused slight rearrangements in the structure and dynamics of the enzyme, acting as a weak inhibitor of Fos binding. Differential scanning fluorimetry (DSF) was used to measure the thermal stability of FosAKP under different conditions, allowing for the selection of a suitable buffer to maximize enzyme operational stability. FosAKP displays absolute specificity towards Fos; therefore, this enzyme was exploited for the development of an enzyme-based colorimetric biosensor. FosAKP was tethered at the bottom of a plastic cuvette using glutaraldehyde chemistry to develop a simple colorimetric method for the determination of Fos in drinking water and animal plasma.
Uptake and Metabolic Conversion of Exogenous Phosphatidylcholines Depending on Their Acyl Chain Structure in Arabidopsis thaliana
Authors: Kotlova Ekaterina R. , Senik Svetlana V. , Pozhvanov Gregory A. , Prokopiev Ilya A. , Boldyrev Ivan A. , Manzhieva Bairta S. , Amigud Ekaterina Ya. , Puzanskiy Roman K. , Khakulova Anna A. , Serebryakov Evgeny B.
Published: December 2023

Fungi and plants are not only capable of synthesizing the entire spectrum of lipids de novo but also possess a well-developed system that allows them to assimilate exogenous lipids. However, the role of structure in the ability of lipids to be absorbed and metabolized has not yet been characterized in detail. In the present work, targeted lipidomics of phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs), in parallel with morphological phenotyping, allowed for the identification of differences in the effects of PC molecular species introduced into the growth medium, in particular, typical bacterial saturated (14:0/14:0, 16:0/16:0), monounsaturated (16:0/18:1), and typical for fungi and plants polyunsaturated (16:0/18:2, 18:2/18:2) species, on Arabidopsis thaliana. For comparison, the influence of an artificially synthesized (1,2-di-(3-(3-hexylcyclopentyl)-propanoate)-sn-glycero-3-phosphatidylcholine, which is close in structure to archaeal lipids, was studied. The phenotype deviations stimulated by exogenous lipids included changes in the length and morphology of both the roots and leaves of seedlings. According to lipidomics data, the main trends in response to exogenous lipid exposure were an increase in the proportion of endogenic 18:1/18:1 PC and 18:1_18:2 PC molecular species and a decrease in the relative content of species with C18:3, such as 18:3/18:3 PC and/or 16:0_18:3 PC, 16:1_18:3 PE. The obtained data indicate that exogenous lipid molecules affect plant morphology not only due to their physical properties, which are manifested during incorporation into the membrane, but also due to the participation of exogenous lipid molecules in the metabolism of plant cells. The results obtained open the way to the use of PCs of different structures as cellular regulators.
C11-hydroxy and C11-oxo C19 and C21 Steroids: Pre-Receptor Regulation and Interaction with Androgen and Progesterone Steroid Receptors
Authors: Gent Rachelle , Van Rooyen Desmaré , Atkin Stephen L. , Swart Amanda C.
Published: December 2023

C11-oxy C19 and C11-oxy C21 steroids have been identified as novel steroids but their function remains unclear. This study aimed to investigate the pre-receptor regulation of C11-oxy steroids by 11β-hydroxysteroid dehydrogenase (11βHSD) interconversion and potential agonist and antagonist activity associated with the androgen (AR) and progesterone receptors (PRA and PRB). Steroid conversions were investigated in transiently transfected HEK293 cells expressing 11βHSD1 and 11βHSD2, while CV1 cells were utilised for agonist and antagonist assays. The conversion of C11-hydroxy steroids to C11-oxo steroids by 11βHSD2 occurred more readily than the reverse reaction catalysed by 11βHSD1, while the interconversion of C11-oxy C19 steroids was more efficient than C11-oxy C21 steroids. Furthermore, 11-ketodihydrotestosterone (11KDHT), 11-ketotestosterone (11KT) and 11β-hydroxydihydrotestosterone (11OHDHT) were AR agonists, while only progestogens, 11β-hydroxyprogesterone (11βOHP4), 11β-hydroxydihydroprogesterone (11βOHDHP4), 11α-hydroxyprogesterone (11αOHP4), 11α-hydroxydihydroprogesterone (11αOHDHP4), 11-ketoprogesterone (11KP4), 5α-pregnan-17α-diol-3,11,20-trione (11KPdione) and 21-deoxycortisone (21dE) exhibited antagonist activity. C11-hydroxy C21 steroids, 11βOHP4, 11βOHDHP4 and 11αOHP4 exhibited PRA and PRB agonistic activity, while only C11-oxo steroids, 11KP4 and 11-ketoandrostanediol (11K3αdiol) demonstrated PRB agonism. While no steroids antagonised the PRA, 11OHA4, 11β-hydroxytestosterone (11OHT), 11KT and 11KDHT exhibited PRB antagonism. The regulatory role of 11βHSD isozymes impacting receptor activation is clear—C11-oxo androgens exhibit AR agonist activity; only C11-hydroxy progestogens exhibit PRA and PRB agonist activity. Regulation by the downstream metabolites of active C11-oxy steroids at the receptor level is apparent—C11-hydroxy and C11-oxo metabolites antagonize the AR and PRB, progestogens the former, androgens the latter. The findings highlight the intricate interplay between receptors and active as well as “inactive” C11-oxy steroids, suggesting novel regulatory tiers.
Differential Effects of Lipid Bilayers on αPSM Peptide Functional Amyloid Formation
Authors: Kristoffersen Kamilla , Hansen Kasper Holst , Andreasen Maria
Published: December 2023

Phenol-soluble modulins (PSMs) are key virulence factors of S. aureus, and they comprise the structural scaffold of biofilm as they self-assemble into functional amyloids. They have been shown to interact with cell membranes as they display toxicity towards human cells through cell lysis, with αPSM3 being the most cytotoxic. In addition to causing cell lysis in mammalian cells, PSMs have also been shown to interact with bacterial cell membranes through antimicrobial effects. Here, we present a study on the effects of lipid bilayers on the aggregation mechanism of αPSM using chemical kinetics to study the effects of lipid vesicles on the aggregation kinetics and using circular dichroism (CD) spectroscopy, Fourier-transform infrared (FTIR) spectroscopy and transmission electron microscopy (TEM) to investigate the corresponding secondary structure of the aggregates. We found that the effects of lipid bilayers on αPSM aggregation were not homogeneous between lipid type and αPSM peptides, although none of the lipids caused changes in the dominating aggregation mechanism. In the case of αPSM3, all types of lipids slowed down aggregation to a varying degree, with 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) having the most pronounced effect. For αPSM1, lipids had opposite effects, where DOPC decelerated aggregation and lipopolysaccharide (LPS) accelerated the aggregation, while 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DOPG) had no effect. For αPSM4, both DOPG and LPS accelerated the aggregation, but only at high concentration, while DOPC showed no effect. None of the lipids was capable of inducing aggregation of αPSM2. Our data reveal a complex interaction pattern between PSMs peptides and lipid bilayers that causes changes in the aggregation kinetics by affecting different kinetic parameters along with only subtle changes in morphology.
Substances Secreted by Lactobacillus spp. from the Urinary Tract Microbiota Play a Protective Role against Proteus mirabilis Infections and Their Complications
Authors: Szczerbiec Dominika , Słaba Mirosława , Torzewska Agnieszka
Published: December 2023

Proteus mirabilis urinary tract infections can lead to serious complications such as development of urinary stones. Lactobacillus spp., belonging to the natural microbiota of the urinary tract, exhibit a number of antagonistic mechanisms against uropathogens, including the secretion of organic acids. In this study, we determined the anti-adhesion, anti-cytotoxicity and anti-crystallization properties of the substances secreted by Lactobacillus. For this purpose, membrane inserts with a pore diameter 0.4 μm were used, which prevent mixing of cultured cells, simultaneously enabling the diffusion of metabolic products. The intensity of crystallization was assessed by measuring the levels of Ca2+, Mg2+ and NH3 and by observing crystals using microscopic methods. The cytotoxicity of the HCV-29 cell line was determined using the LDH and MTT assays, and the impact of lactobacilli on P. mirabilis adhesion to the bladder epithelium was assessed by establishing CFU/mL after cell lysis. It was shown that in the presence of L. gasseri the adhesion of P. mirabilis and the cytotoxicity of the cells decreased. The degree of crystallization was also inhibited in all experimental models. Moreover, it was demonstrated that L. gasseri is characterized by the secretion of a high concentration of L-lactic acid. These results indicate that L-lactic acid secreted by L. gasseri has a significant impact on the crystallization process and pathogenicity of P. mirabilis.
Methylation of the Vitamin D Receptor Gene in Human Disorders
Authors: Gasperini Beatrice , Falvino Angela , Piccirilli Eleonora , Tarantino Umberto , Botta Annalisa , Visconti Virginia Veronica
Published: December 2023

The Vitamin D Receptor (VDR) mediates the actions of 1,25-Dihydroxvitamin D3 (1,25(OH)2D3), which has important roles in bone homeostasis, growth/differentiation of cells, immune functions, and reduction of inflammation. Emerging evidences suggest that epigenetic modifications of the VDR gene, particularly DNA methylation, may contribute to the onset and progression of many human disorders. This review aims to summarize the available information on the role of VDR methylation signatures in different pathological contexts, including autoimmune diseases, infectious diseases, cancer, and others. The reversible nature of DNA methylation could enable the development of therapeutic strategies, offering new avenues for the management of these worldwide diseases.
Effects of Ionic Liquid Content on the Electrical Properties of PVDF Films by Fused Deposition Modeling
Authors: Zhou Runkai , Yang Hong , Zhao Lianzhong , Wang Chun , Peng Chaoqun , Wang Richu , Zhang Dou , Wang Xiaofeng
Published: December 2023

In this study, polyvinylidene fluoride (PVDF) composite films were prepared by fused deposition modeling, and the effects of ionic liquid (IL) content on the printability, crystallization behavior, and electrical properties of melted PVDF were systematically investigated. The results show that the addition of IL increased the temperature sensitivity of melted PVDF and decreased its viscosity, while IL acted as a plasticizer to lower the melting point of PVDF and improve its FDM printability. The imidazole cations in IL had electrostatic interactions with the -CF2- groups in PVDF, which promoted the transformation of the nonpolar phase to the polar phase in PVDF; thus, the addition of IL was beneficial to the increase in the polar β phase. The PVDF with 20 wt.% IL contained the highest proportion of β phase content (32.59%). Moreover, the increase in polar β-phase content also increased the polarization strength of PVDF and improved its ferroelectric properties. PVDF with 10 wt.% IL had the highest residual polarization strength (16.87 μC/m2).
Preparation of Electrodes with β-Nickel Hydroxide/CVD-Graphene/3D-Nickel Foam Composite Structures to Enhance the Capacitance Characteristics of Supercapacitors
Authors: Lu Yang-Ming , Hong Sheng-Huai
Published: December 2023

Supercapacitors have the characteristics of high power density, long cycle life, and fast charge and discharge rates, making them promising alternatives to traditional capacitors and batteries. The use of transition-metal compounds as electrode materials for supercapacitors has been a compelling research topic in recent years because their use can effectively enhance the electrical performance of supercapacitors. The current research on capacitor electrode materials can mainly be divided into the following three categories: carbon-based materials, metal oxides, and conductive polymers. Nickel hydroxide (Ni(OH)2) is a potential electrode material for use in supercapacitors. Depending on the preparation conditions, two crystal phases of nickel hydroxide, α and β, can be produced. When compared to α-NiOH, the structure of β-Ni(OH)2 does not experience ion intercalation. As a result, the carrier transmission rate of α-Ni(OH)2 is slower, and its specific capacitance value is smaller. Its carrier transport rate can be improved by adding conductive materials, such as graphene. β-Ni(OH)2 was chosen as an electrode material for a supercapacitor in this study. Homemade low-pressure chemical vapor deposition graphene (LPCVD-Graphene) conductive material was introduced to modify β-Ni(OH)2 in order to increase its carrier transport rate. The LPCVD method was used to grow high-quality graphene films on three-dimensional (3D) nickel foam substrates. Then, a hydrothermal synthesis method was used to grow β-Ni(OH)2 nanostructures on the 3D graphene/nickel foam substrate. In order to improve the electrical properties of the composite structure, a high-quality graphene layer was incorporated between the nickel hydroxide and the 3D nickel foam substrate. The effect of the conductive graphene layer on the growth of β-Ni(OH)2, as well as its electrical properties and electrochemical performance, was studied. When this β-Ni(OH)2/CVD-Graphene/3D-NF (nickel foam) material was used as the working electrodes of the supercapacitor under a current density of 1 A/g and 3 A/g, they exhibited a specific capacitance of 2015 F/g and 1218.9 F/g, respectively. This capacitance value is 2.62 times higher than that of the structure without modification with a graphene layer. The capacitance value remains at 99.2% even after 1000 consecutive charge and discharge cycles at a current density of 20 A/g. This value also improved compared to the structure without graphene layer modification (94.7%).
Thermo-Responsive Shape-Memory Dual-Cured Polymers Based on Vegetable Oils
Authors: Petrauskas Rokas , Grauzeliene Sigita , Ostrauskaite Jolita
Published: December 2023

The development of thermo-responsive shape-memory polymers has attracted attention due to their ability to undergo reversible deformations based on temperature changes. Vegetable oils are confirmed to be an excellent biorenewable source of starting materials for the synthesis of polymers. Therefore, the objective of this research was to synthesize thermo-responsive shape-memory polymers based on vegetable oils by using the dual-curing technique and obtaining polymers with tailorable properties. Acrylated epoxidized soybean oil and two epoxidized vegetable oils, linseed oil and camelina oil, were chosen for dual curing with m-xylylenediamine. Rheological tests were used to analyze the curing kinetics of systems undergoing radical photopolymerization, thermal cationic polymerization, and dual-curing processes. The rheological, mechanical, and thermal characteristics of the polymers were enhanced by the second curing stage. Dual-cured vegetable oil-based polymers had shape-memory properties with a recovery ratio of 100%, making them suitable for a variety of applications, including electronics, biomedical devices, and robotics.
Incorporation of Cellulose-Based Aerogels into Textile Structures
Authors: Sozcu Sebnem , Venkataraman Mohanapriya , Wiener Jakub , Tomkova Blanka , Militky Jiri , Mahmood Aamir
Published: December 2023

Given their exceptional attributes, aerogels are viewed as a material with immense potential. Being a natural polymer, cellulose offers the advantage of being both replenishable and capable of breaking down naturally. Cellulose-derived aerogels encompass the replenish ability, biocompatible nature, and ability to degrade naturally inherent in cellulose, along with additional benefits like minimal weight, extensive porosity, and expansive specific surface area. Even with increasing appreciation and acceptance, the undiscovered possibilities of aerogels within the textiles sphere continue to be predominantly uninvestigated. In this context, we outline the latest advancements in the study of cellulose aerogels’ formulation and their diverse impacts on textile formations. Drawing from the latest studies, we reviewed the materials used for the creation of various kinds of cellulose-focused aerogels and their properties, analytical techniques, and multiple functionalities in relation to textiles. This comprehensive analysis extensively covers the diverse strategies employed to enhance the multifunctionality of cellulose-based aerogels in the textiles industry. Additionally, we focused on the global market size of bio-derivative aerogels, companies in the industry producing goods, and prospects moving forward.
Influencing Factors of Porosity and Strength of Plant-Growing Concrete
Authors: Cai Jiashi , Shen Chunying , Ye Ming , Huang Siyang , He Jinxing , Cui Ding
Published: December 2023

A standardized preparation process is proposed in this study for achieving optimal strength and vegetative properties in vegetated concrete, using Yunnan red soil as a growth substrate for plants. The porosity of vegetated concrete is a crucial factor influencing plant growth, while compressive strength is a significant mechanical property. To assess the strength and porosity of vegetated concrete, different design porosities (22%, 24%, 26%, 28%) and cement-to-aggregate ratios (4, 5, 6, 7) were utilized in the preparation of vegetated concrete samples. The shell-making and static-pressure-molding methods were optimized for specimen preparation. Analyzing the stress–strain full curve characteristics of vegetation-type concrete under different influencing factors, an in-depth investigation into its failure mechanism was conducted. It was determined that the design porosity and cement content significantly impact the concrete’s performance, particularly in terms of 30-day compressive strength and effective porosity. Furthermore, an increase in the fly ash ratio led to an increase in porosity and a decrease in compressive strength, providing a certain guidance for optimizing concrete performance. Comparative analysis through vegetation experiments revealed that black rye grass exhibited favorable growth adaptability compared to other grass species.
Tailoring the Coefficient of Friction by Direct Laser Writing Surface Texturing
Authors: Gaudiuso Caterina , Volpe Annalisa , Mezzapesa Francesco Paolo , Putignano Carmine , Ancona Antonio
Published: December 2023

The modification of the surface topography at the micro- and nanoscale is a widely established as one of the best ways to engineering the surface of materials, to improve the tribological performances of materials in terms of load capacity and friction. The present paper reviews the state of the art on laser surface texturing by exploiting the technique of direct laser writing for tailoring the coefficient of friction, highlighting the effect of the textures’ arrangement on the lubricated conformal and non-conformal contact behavior.
Suitability of Embedded Liquid Cooling and Heat Generation for Chips
Authors: Zhang Jian , Wu Jiechang , Xie Zhihui , Lu Zhuoqun , Guan Xiaonan , Ge Yanlin
Published: December 2023

Embedded liquid cooling is a preferred solution for dissipating the heat generated by high-power chips. The cooling capacity and pump power consumption of embedded liquid cooling heat sinks differ significantly between different structures. To achieve an accurate match between cooling capacity and heat dissipation requirements, the selection of a liquid-cooled heat sink should be carefully considered in conjunction with the heat dissipation needs of heat sources in real-world thermal management issues. Based on the manufacturing limitations on chip temperature and microchannel pressure, a composite performance index function was developed to assess the cooling capacity and cooling cost of the heat sink. This allowed for the establishment of an evaluation standard to determine the suitability of embedded liquid cooling and heat sink for the heat source. In this study, the suitability of four microchannel heat sinks with the same feature length and fin volume was evaluated under various thermal load conditions. The results show that the best-suited heat sink changes with variations in the thermal load of the chip. In the example, when the heat source was homogeneous at 100 W, the circular section pin fins have an optimal suitability of 0.928 for Re = 500. When the heat source was a heterogeneous heat source with a power of 100 W, the value of Θ was found to be 0.389. Additionally, the optimal suitability of drop section pin fins for Re = 971.5 was determined to be 0.862.
Ameliorative Effect of Ginsenoside Rg6 in Periodontal Tissue Inflammation and Recovering Damaged Alveolar Bone
Authors: Lee Won-Jin , Kim Eun-Nam , Trang Nguyen Minh , Lee Jee-Hyun , Cho Soo-Hyun , Choi Hui-Ji , Song Gyu-Yong , Jeong Gil-Saeng
Published: December 2023

Periodontal disease is a chronic disease with a high prevalence, and in order to secure natural materials to prevent oral diseases, new materials that protect periodontal tissue from inflammation are being sought. Genes were identified using real-time quantitative polymerase chain reaction (RT-qPCR), and proteins were confirmed using Western blot. Dichlorodihydrofluorescein diacetate (DCF-DA) analysis was used, and the antibacterial effects were confirmed through Minimum Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) analysis. To confirm this effect in vivo, Sprague–Dawley rats, in which periodontitis was induced using ligation or Lipopolysaccharide of Porphyromonas gingivalis (PG-LPS), were used. In vitro experiments using human periodontal ligament (HPDL) cells stimulated with PG-LPS showed that Ginsenoside Rg6 (G-Rg6) had anti-inflammatory, antibacterial, antioxidant, and osteoblast differentiation properties. In vivo, G-Rg6 was effective in Sprague–Dawley rats in which periodontitis was induced using ligation or PG-LPS. Therefore, Ginsenoside Rg6 shows potential effectiveness in alleviating periodontitis.
Nutritional Assessments by Bioimpedance Technique in Dialysis Patients
Authors: Ng Jack Kit-Chung , Lau Sam Lik-Fung , Chan Gordon Chun-Kau , Tian Na , Li Philip Kam-Tao
Published: December 2023

Bioelectrical impedance analysis (BIA) has been extensively applied in nutritional assessments on the general population, and it is recommended in establishing the diagnosis of malnutrition and sarcopenia. The bioimpedance technique has become a promising modality through which to measure the whole-body composition in dialysis patients, where the presence of subclinical volume overload and sarcopenic obesity may be overlooked by assessing body weight alone. In the past two decades, bioimpedance devices have evolved from applying a single frequency to a range of frequencies (bioimpedance spectroscopy, BIS), in which the latter is incorporated with a three-compartment model that allows for the simultaneous measurement of the volume of overhydration, adipose tissue mass (ATM), and lean tissue mass (LTM). However, clinicians should be aware of common potential limitations, such as the adoption of population-specific prediction equations in some BIA devices. Inherent prediction error does exist in the bioimpedance technique, but the extent to which this error becomes clinically significant remains to be determined. Importantly, reduction in LTM has been associated with increased risk of frailty, hospitalization, and mortality in dialysis patients, whereas the prognostic value of ATM remains debatable. Further studies are needed to determine whether modifications of bioimpedance-derived body composition parameters through nutrition intervention can result in clinical benefits.
Efficacy of Probiotic Supplements on Brain-Derived Neurotrophic Factor, Inflammatory Biomarkers, Oxidative Stress and Cognitive Function in Patients with Alzheimer’s Dementia: A 12-Week Randomized, Double-Blind Active-Controlled Study
Authors: Hsu Yu-Chieh , Huang Yen-Yu , Tsai Shin-Yu , Kuo Yi-Wei , Lin Jia-Hung , Ho Hsieh-Hsun , Chen Jui-Fen , Hsia Ko-Chiang , Sun Yu
Published: December 2023

The role of neurotrophic factors, oxidative stress, and inflammation in the pathogenesis of Alzheimer’s disease (AD) has been explored. Animal studies have reported the positive effects of probiotics on these factors. Some clinical studies also support the potential role of probiotics in improving cognitive function via the gut–brain axis in older adults. However, clinical experimental studies evaluating the efficacy of probiotics targeting the neurotrophic factors and inflammatory biomarkers, particularly among AD patients, remain very limited. In this randomized, double-blinded, active-controlled trial, we used multi-strain probiotic supplements, including Bifidobacterium longum subsp. infantis BLI-02, B. breve Bv-889, B. animalis subsp. lactis CP-9, B. bifidum VDD088, and Lactobacillus plantarum PL-02 as the intervention. Participants were divided into an active control group (received probiotic supplements containing 5 × 107 colony-forming units per day, CFU/day) and a treatment group (1 × 1010 CFU/day). Student’s t test was applied as the main method of statistical analysis. After 12 weeks of intervention, the treatment group demonstrated a 36% increase in serum brain-derived neurotrophic factor (BDNF) (* p = 0.005), a reduction in IL-1β (* p = 0.041), and an increase in antioxidant superoxide dismutase (SOD) (* p = 0.012). No significant change was found in the active control group. A trend toward less cognitive deterioration was observed, but not statistically significant. In conclusion, this study presents evidence supporting the benefits of multi-strain probiotics in enhancing BDNF, ameliorating inflammation and oxidative stress in AD patients.
Compositional Aspects of Beverages Designed to Promote Hydration Before, During, and After Exercise: Concepts Revisited
Authors: Pérez-Castillo Íñigo M. , Williams Jennifer A. , López-Chicharro José , Mihic Niko , Rueda Ricardo , Bouzamondo Hakim , Horswill Craig A.
Published: December 2023

Hypohydration can impair aerobic performance and deteriorate cognitive function during exercise. To minimize hypohydration, athletes are recommended to commence exercise at least euhydrated, ingest fluids containing sodium during long-duration and/or high-intensity exercise to prevent body mass loss over 2% and maintain elevated plasma osmolality, and rapidly restore and retain fluid and electrolyte homeostasis before a second exercise session. To achieve these goals, the compositions of the fluids consumed are key; however, it remains unclear what can be considered an optimal formulation for a hydration beverage in different settings. While carbohydrate–electrolyte solutions such as sports drinks have been extensively explored as a source of carbohydrates to meet fuel demands during intense and long-duration exercise, these formulas might not be ideal in situations where fluid and electrolyte balance is impaired, such as practicing exercise in the heat. Alternately, hypotonic compositions consisting of moderate to high levels of electrolytes (i.e., ≥45 mmol/L), mainly sodium, combined with low amounts of carbohydrates (i.e., <6%) might be useful to accelerate intestinal water absorption, maintain plasma volume and osmolality during exercise, and improve fluid retention during recovery. Future studies should compare hypotonic formulas and sports drinks in different exercise settings, evaluating different levels of sodium and/or other electrolytes, blends of carbohydrates, and novel ingredients for addressing hydration and rehydration before, during, and after exercise.
Animal Research: Transparency and Obligations
Authors: Fernandez Maria Luz , Serra-Majem Lluis
Published: December 2023

As Editors-in-Chief of Nutrients—one of the largest nutrition journals in the world—we would like to comment on this subject because it will remain topical for as long as it exists [...]
Topical Insulin in Neurotrophic Keratopathy: A Review of Current Understanding of the Mechanism of Action and Therapeutic Approach
Authors: Jaworski Marcin , Lorenc Anna , Leszczyński Rafał , Mrukwa-Kominek Ewa
Published: December 2023

Neurotrophic keratopathy is a corneal disease characterized by impaired corneal innervation. It can lead to corneal epithelial defects, ulcerations, and perforations. Topical insulin has been shown to be effective in treating this disorder. Insulin is a growth factor that can promote corneal epithelial cell proliferation and migration. In addition, it can also inhibit corneal epithelial cell apoptosis. Topical insulin has previously been found to enhance corneal wound healing. This article reviews the current understanding of the mechanism of action of topical insulin in the treatment of neurotrophic keratopathy.
High-Cycle Fatigue Behaviour of Polyetheretherketone (PEEK) Produced by Additive Manufacturing
Authors: Rendas Pedro , Imperadeiro Alexandre , Martins Rui F. , Soares Bruno A. R.
Published: December 2023

Polyetheretherketone (PEEK) is the leading high-performance thermoplastic biomaterial that can be processed through material extrusion (ME) additive manufacturing (AM), also known as three-dimensional (3D) printing, for patient-specific load-bearing implant manufacture. Considering the importance of cyclic loading for load-bearing implant design, this work addresses the high-cycle fatigue behaviour of 3D-printed PEEK. In this work, printed PEEK specimens are cyclically loaded under stress-controlled tension–tension using different stress levels between 75% and 95% of printed PEEK’s tensile strength. The experimental results are used to document 3D-printed PEEK’s fatigue behaviour using Basquin’s power law, which was compared with previous fatigue research on bulk PEEK and other 3D-printing materials. As a pioneering study on its fatigue behaviour, the results from this work show that 3D-printed PEEK exhibits an above-average fatigue strength of 65 MPa, corresponding to about 75% of its tensile strength. Fracture surface analysis suggests that a transition can occur from ductile to brittle fracture with maximum stresses between 85% and 95% of the tensile strength. Evidence of crack propagation features on fracture surfaces under scanning electron microscope (SEM) observation suggests crack initiation in void defects created by printing deposition that propagates longitudinally through line bonding interfaces along layers. Considering this, 3D-printed PEEK’s fatigue behaviour can be strongly related to printing conditions. Further research on the fatigue behaviour of 3D-printed PEEK is necessary to support its use in load-bearing implant applications.
A New Microwave-Assisted Protocol for Cellulose Extraction from Eucalyptus and Pine Tree Wood Waste
Authors: Vinhas Silvia , Sarraguça Mafalda , Moniz Tânia , Reis Salette , Rangel Maria
Published: December 2023

An enormous interest in the development of efficient protocols for cellulose extraction has been demonstrated in the last few years, although usually based on non-sustainable chemical and thermal approaches. In this work, we propose a new and more sustainable method for cellulose extraction from eucalyptus and pine tree wood waste products exclusively performed using microwave-assisted radiation. The methodology includes three main steps: (i) alkaline treatment; (ii) bleaching I, using H2O2; and (iii) bleaching II, an acidic treatment. Samples obtained in each step were characterized by Fourier-transform Infrared (FTIR) spectroscopy, powder X-ray diffraction (PXRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). The results were compared with the structural and thermal profile of the starting materials, a commercially available microcrystalline cellulose and with an industrial paper pulp sample. Results confirmed that for both types of wood wastes, cellulose was retained during the extraction procedures and that the removal of hemicellulose and lignin was mainly achieved in the last step, as seen by the FTIR spectra and TGA curves. The developed protocol is innovative, as it constitutes an easy and quick approach for extracting cellulose from eucalyptus and pine tree wood waste. Mild chemical and thermal conditions are used during the three extraction steps (microwave irradiation, aqueous solutions, maximum of 120 °C in a total of 3 h). Moreover, environmentally friendly purification steps are applied based on the use of water and ethanol. This approach offers the possibility of a future scale-up study to potentially apply the developed protocol to the extraction of cellulose on an industrial scale.
Electrospun Nanofiber Scaffolds Loaded with Metal-Based Nanoparticles for Wound Healing
Authors: Dang Zheng , Ma Xuemei , Yang Zihao , Wen Xiaohu , Zhao Pengxiang
Published: December 2023

Failures of wound healing have been a focus of research worldwide. With the continuous development of materials science, electrospun nanofiber scaffolds loaded with metal-based nanoparticles provide new ideas and methods for research into new tissue engineering materials due to their excellent antibacterial, anti-inflammatory, and wound healing abilities. In this review, the stages of extracellular matrix and wound healing, electrospun nanofiber scaffolds, metal-based nanoparticles, and metal-based nanoparticles supported by electrospun nanofiber scaffolds are reviewed, and their characteristics and applications are introduced. We discuss in detail the current research on wound healing of metal-based nanoparticles and electrospun nanofiber scaffolds loaded with metal-based nanoparticles, and we highlight the potential mechanisms and promising applications of these scaffolds for promoting wound healing.
Effects of Copper(II) Oxide on the Co-Pyrolysis of Waste Polyester Enameled Wires and Poly(vinyl chloride)
Authors: Wang Xiaolu , Li Bingyi , Xia Zhidong , Zhou Wei , Wu Yufeng , Zhu Zhaoxi , Zhu Guangze
Published: December 2023

The emission of chlorinated pollutants is one of the main problems when recovering copper (Cu) via pyrolysis from waste enameled wires. This is mainly attributed to other wastes which possess high poly(vinyl chloride) content, such as electrical wires and cables, which are often recycled together with enameled copper wires. In this research, to control the chlorinated pollutants, copper(II) oxide (CuO) was chosen and demonstrated to be an efficient dechlorinating agent, and CuO did not introduce any impurities that influence the quality of the recovered Cu. The pyrolysis and co-pyrolysis of polyester enameled wires, PVC, and CuO were investigated, and special attention was paid to chlorinated compounds in released pyrolytic products. In particular, the co-pyrolysis of this ternary mixture was studied for the first time, and some new pyrolysis behaviors were discovered. For example, the results of Py-GC/MS analyses showed that the addition of CuO removed about 75% of the chloro-organic products, the main types of which were chloroaromatic compounds rather than the more toxic chloroesters. Moreover, pyrolysis gases were collected and characterized via ion chromatography, and the results showed that the chlorine content in the pyrolysis gases decreased by about 71%. TG analysis indicated that CuO only minimally affected the pyrolysis of polyester paint. However, through the chlorine fixation effect, CuO influenced the dechlorination and dehydrochlorination of PVC, as well as secondary reactions between HCl and pyrolysis products of polyester paint, therefore changing the products and behaviors of co-pyrolysis. Mechanism of reducing chlorine-containing pollutants and reaction mechanism of forming typical pyrolysis products closely correlated to the effects of CuO were also proposed, providing theoretical guidance for the recycling of waste enameled wires.
Supercritical Antisolvent Precipitation of Corticosteroids/β-Cyclodextrin Inclusion Complexes
Authors: Mottola Stefania , De Marco Iolanda
Published: December 2023

In this study, corticosteroid–β-cyclodextrin (β-CD) inclusion complexes were prepared by using supercritical antisolvent (SAS) precipitation to enhance the dissolution rate of dexamethasone (DEX) and prednisolone (PRED), which are poorly water soluble drugs. The processing of the active principles in the absence of a carrier led to their almost complete extraction (the small amount of obtained material precipitates in the form of crystals). The coprecipitation of the ingredients in the presence of β-CD was investigated at different concentrations, pressures, and molar ratios. For both the corticosteroids, the optimized operating conditions were 40 °C, 120 bar, an equimolar ratio, and a concentration in DMSO of 20 mg/mL; these conditions led to the attainment of microparticles with mean diameters equal to 0.197 ± 0.180 μm and 0.131 ± 0.070 μm in the case of DEX and PRED, respectively. Job’s method confirmed the formation of inclusion complexes with a 1/1 mol/mol ratio. Compared to the pure ingredients, the obtained powders have an improved release rate, which is about three times faster in both cases. The release curves obtained under the best operating conditions were fitted using different models. The best fitting was obtained using the Weibull model, whose parameters are compatible with a combined release mechanism involving Fickian diffusion and controlled release.
Spatiotemporal Evolution and Rank–Size Pattern of Chinese Urban Settlements
Authors: Zhang Jing , Li Chunlin , Zhang Baolei , Hu Yuanman , Wang Hao , Li Zhenxing , Zhang Qian
Published: December 2023

Accurate and timely urban boundaries can effectively quantify the spatial characteristics of urban evolution and are essential for understanding the impacts of urbanization processes and land-use changes on the environment and biodiversity. Currently, there is a lack of long time-series, high-resolution, nationally consistent Chinese urban boundary data for urban research. In this study, the city clustering algorithm was used to generate urban settlement boundaries in China based on the local density, size, and spatial relationships of impervious surfaces. The results showed that both the area and the number of urban settlements in China revealed an upward trend from 1985 to 2020, with East China (EC) being much higher than other regions and South China showing the most significant growth rate. The average area ratio of urban green space in China was 41.68%, with the average area ratio in EC being higher than in other regions. Meanwhile, Zipf’s law was used to verify the universality of urban settlement rank–size; the changes in the Zipf index from 1985 to 2020 also revealed that China’s urban size tended to be concentrated, and the development of large urban settlements was relatively prominent. The urban definition method we propose in this study can divide urban boundaries efficiently and accurately, identify urban expansion hotspots, and promote research on farmland loss and ecological land degradation, further exploring the impacts of urbanization on food security, biodiversity, and carbon sequestration. By coupling big data such as economy, energy, and population with urban evolution patterns, urban managers can analyze current and future problems in urban development, thereby providing scientific decision-making for urban sustainability.