bluuebunny/crossref_metadata_2025 huggingface dataset:
Enlarging the Arsenal of Test Species for Sediment Quality Assessment
Authors: Wieringa N. , Droge S. T. J. , Bakker A. M. , Melkert R. A. , Prast B. J. , Verdonschot P. F. M. , Kraak M. H. S.
Published: February 2023

Since only a few standard benthic test species are available for sediment quality, our study aimed to employ multiple test species representing different sensitivity categories in the quality assessment of contaminated sediments. To this end three macroinvertebrate species, Sericostoma personatum (caddisfly, sensitivity category 10), Asellus aquaticus (isopod, category 3) and Chironomus riparius (chironomid, category 2), were exposed to sediments originating from various contamination sources in whole sediment bioassays using intact sediment cores. The agricultural sediment caused insect mortality, the agricultural and urban sediment caused isopod growth reduction and the urban and Wastewater Treatment Plant (WWTP) sediment affected chironomid emergence time. It is concluded that the arsenal of standard species can be successfully expanded by non-standard species, reducing over- or underestimation of the risks of contaminated sediments.
Retrospective analysis of apical prolapse correction by unilateral pectineal suspension: perioperative and short-term results
Authors: Bolovis Dimitrios Ilias , Schreibmayer Michael , Hitzl Wolfgang , Brucker Cosima Veronika Maria
Published: February 2023

Introduction and hypothesis

We have previously published the novel method of unilateral pectineal suspension (UPS) for apical prolapse correction. UPS provides mesh-free midline uterus suspension using a single non-absorbable suture to attach the anterior cervix to the lateral part of the iliopectineal ligament. The purpose of this retrospective cohort study was to analyze the short-term efficacy, perioperative complication rate, and overall patient acceptance of the new UPS surgical concept.

Methods

Forty-seven patients with POP-Q stage 2–4 who underwent robotic UPS between January 1, 2020 and December 31, 2021 were included in the study. Patient data were taken retrospectively from the patient files. Treatment success was the primary endpoint, measured both objectively using a defined composite endpoint and subjectively according to patients’ acceptance 3–6 months after surgery during a follow-up examination. Secondary outcome measures included complications and conversions, and effect of additional procedures on operative time.

Results

Treatment success as measured by the defined composite endpoint was 93.6% for the entire cohort. No complications or conversions occurred. Mean operation time for isolated UPS was 46.5 min (n = 33 patients). UPS can be easily combined with additional surgical procedures for repair of remaining pelvic floor defects, incontinence surgery or other indications. Additional procedures performed had a significant influence on operation time (p < 0.0005, n = 14).

Conclusions

UPS shows highly favorable results when looking at an unselected cohort of patients in need of primary POP surgery with respect to established quality parameters of POP repair.
Evidential requirements for the regulatory hazard and risk assessment of respiratory sensitisers: methyl methacrylate as an example
Authors: Meek Bette , Bridges James W. , Fasey Andrew , Sauer Ursula G.
Published: February 2023

This review addresses the need for a framework to increase the consistency, objectivity and transparency in the regulatory assessment of respiratory sensitisers and associated uncertainties. Principal issues are considered and illustrated through a case study (with methyl methacrylate). In the absence of test methods validated for regulatory use, formal documentation of the weight-of-evidence for hazard classification both at the level of integration of individual studies within lines of evidence and across a broad range of data streams was agreed to be critical for such a framework. An integrated approach is proposed to include not only occupational studies and clinical evidence for the regulatory assessment of respiratory sensitisers, but also information on structure and physical and chemical factors, predictive approaches such as structure activity analysis and in vitro and in vivo mechanistic and toxicokinetic findings. A weight-of-evidence protocol, incorporating integration of these sources of data based on predefined considerations, would contribute to transparency and consistency in the outcome of the assessment. In those cases where a decision may need to be taken on the basis of occupational findings alone, conclusions should be based on transparent weighting of relevant data on the observed prevalence of occupational asthma in various studies taking into account all relevant information including the range and nature of workplace exposures to the substance of interest, co-exposure to other chemicals and study quality.
The influence of the texture and color of goat’s salad cheese on the emotional reactions of consumers compared to cow’s milk cheese and Feta cheese
Authors: Kaczyński Łukasz K. , Cais-Sokolińska Dorota , Bielska Paulina , Teichert Joanna , Biegalski Jakub , Yiğit Aslı , Chudy Sylwia
Published: February 2023

In this study, the sensory and mechanical aspects of the texture of goat’s milk salad cheese were correlated with the emotional profiles of consumers. Using descriptive sensory analysis and instrumental assessment, the texture profile of goat’s milk salad cheese was compared to cow’s milk salad cheese and Feta cheese. Texture measurements confirmed that goat’s cheese compared to cow’s cheese had more softness and less hardness, and Feta cheese had the highest whiteness index compared to the other cheeses. Goat’s milk salad cheese was much less acceptable to consumers compared to cow’s milk cheese and Feta cheese. Consumers also indicated that the hardness of goat’s cheese was lower than that of cow’s cheese and Feta cheese. A reduction in “stickiness” in comparison with cow’s cheese was also reported; however, it was much higher than that for Feta cheese. The “fracturability” and “graininess” of goat’s cheese was similar to cow’s cheese. Emotional profile analysis showed that goat’s cheese evokes mainly negative emotions. Consumers indicated only one positive emotion in the case of this cheese, which was “healthy”. The most frequently mentioned emotions after the consumption of goat’s cheese were “upset”, “disgusted” and “worried”. Many consumers also indicated “disappointed” and “angry”, which did not occur after the consumption of cow’s cheese. This research shows how important it is to combine several analyses and techniques when evaluating dairy products, including salad cheeses. It is also important that consumer research is enriched by emotional profiling.

Graphical abstract
Spatial and temporal (non)binding of audiovisual rhythms in sensorimotor synchronisation
Authors: Lapenta Olivia Morgan , Keller Peter E. , Nozaradan Sylvie , Varlet Manuel
Published: February 2023

Human movement synchronisation with moving objects strongly relies on visual input. However, auditory information also plays an important role, since real environments are intrinsically multimodal. We used electroencephalography (EEG) frequency tagging to investigate the selective neural processing and integration of visual and auditory information during motor tracking and tested the effects of spatial and temporal congruency between audiovisual modalities. EEG was recorded while participants tracked with their index finger a red flickering (rate fV = 15 Hz) dot oscillating horizontally on a screen. The simultaneous auditory stimulus was modulated in pitch (rate fA = 32 Hz) and lateralised between left and right audio channels to induce perception of a periodic displacement of the sound source. Audiovisual congruency was manipulated in terms of space in Experiment 1 (no motion, same direction or opposite direction), and timing in Experiment 2 (no delay, medium delay or large delay). For both experiments, significant EEG responses were elicited at fV and fA tagging frequencies. It was also hypothesised that intermodulation products corresponding to the nonlinear integration of visual and auditory stimuli at frequencies fV ± fA would be elicited, due to audiovisual integration, especially in Congruent conditions. However, these components were not observed. Moreover, synchronisation and EEG results were not influenced by congruency manipulations, which invites further exploration of the conditions which may modulate audiovisual processing and the motor tracking of moving objects.
ERCC6L2-related disease: a novel entity of bone marrow failure disorder with high risk of clonal evolution
Authors: Baccelli Francesco , Leardini Davide , Cerasi Sara , Messelodi Daria , Bertuccio Salvatore Nicola , Masetti Riccardo
Published: February 2023

ERCC excision repair 6 like 2 (ERCC6L2) gene encodes for different helicase-like protein members of the Snf2 family involved in transcription-coupled nucleotide excision repair and in cell proliferation. Germline homozygous mutations in children and adults predispose to a peculiar bone marrow failure phenotype characterized by mild hematological alterations with a high risk of developing acute myeloid leukemia. The outcome for patients with leukemia progression is dismal while patients undergoing hematopoietic stem cell transplantation in the early stage have better outcomes. The ERCC6L2-related hematological disease presents a high penetrance, posing important questions regarding the treatment strategies and possible preemptive approaches. This review describes the biological function of ERCC6L2 and the clinical manifestations of the associated disease, trying to focus on the unsolved clinical questions.
A systematic review of the incidence, management and prognosis of new-onset autoimmune connective tissue diseases after COVID-19
Authors: Kouranloo Koushan , Dey Mrinalini , Elwell Helen , Nune Arvind
Published: February 2023

A literature review on new-onset autoimmune connective tissue diseases (ACTDs) following COVID-19 is lacking. We evaluated potential associations between COVID-19 and the development of new-onset ACTDs. The “population” was adults with disease terms for ACTDs, including systemic lupus erythematosus (SLE), Sjogren’s syndrome, systemic sclerosis (SSc), idiopathic inflammatory myositis (IIM), anti-synthetase syndrome, mixed CTD and undifferentiated CTD, and “intervention” as COVID-19 and related terms. Databases were searched for English-language articles published until September 2022. We identified 2236 articles with 28 ultimately included. Of the 28 included patients, 64.3% were female, with a mean age was 51.1 years. The USA reported the most cases (9/28). ACTD diagnoses comprised: 11 (39.3%) IIM (including four dermatomyositis); 7 (25%) SLE; four (14.3%) anti-synthetase syndrome; four (14.3%) SSc; two (7.1%) other ACTD (one lupus/MCTD overlap). Of eight, four (14.3%) patients (including that with lupus/MCTD) had lupus nephritis. The average time from COVID-19 to ACTD diagnosis was 23.7 days. A third of patients were admitted to critical care, one for treatment of haemophagocytic lymphohistiocytosis in SLE (14 sessions of plasmapheresis, rituximab and intravenous corticosteroids) and nine due to COVID-19. 80% of patients went into remission of ACTD following treatment, while three (10%) patients died—one due to macrophage activation syndrome with anti-synthetase syndrome and two from unreported causes. Our results suggest a potential association between COVID-19 and new-onset ACTDs, notably in young females, reflecting more comprehensive CTD epidemiology. The most common diagnosis in our cohort was IIM. The aetiology and mechanisms by which ACTDs emerge following COVID-19 remain unknown and require further research.
Cryogenic Tm:LiYF4 laser around 2 µm
Authors: Alles Adrian , Jambunathan Venkatesan , Slimi Sami , Serres Josep M. , Aguiló Magdalena , Díaz Francesc , Mateos Xavier , Smrz Martin , Mocek Tomas
Published: February 2023

We present the laser characteristics of a 5 at.% Tm:YLF crystal using a modular setup at cryogenic temperatures emitting around 2 µm. Continuous-wave laser operation was achieved by pumping the laser crystal using a Volume Bragg Grating-stabilized laser diode emitting at 793 nm. A maximum output power of 6.5 W was achieved at 80 K corresponding to a slope efficiency of 66.0% with respect to the absorbed power with excellent beam quality.
Positional preferences and efficiency in a dynamic economy
Authors: Aronsson Thomas , Ghosh Sugata , Wendner Ronald
Published: February 2023

In an endogenous growth model, we characterize the conditions under which positional preferences for consumption and wealth do not cause inefficiency and derive an optimal tax policy response in cases where these conditions are not satisfied. The concerns for relative consumption and relative wealth partly emanate from social comparisons with people in other countries. We distinguish between a (conventional) welfarist government and a non-welfarist government that does not attach any social value to relative concerns. We also compare the outcome of Nash-competition among local/national governments with the resource allocation implied by a global social optimum both under welfarism and non-welfarism.
Relation between laxative use and risk of major bleeding in patients with atrial fibrillation and heart failure
Authors: Yamamoto Jumpei , Yamamoto Masaya , Hara Hisao , Hiroi Yukio
Published: February 2023

Constipation has been reported to increase the risk of cardiovascular mortality. Patients with atrial fibrillation (AF) and heart failure (HF) have more comorbidities and an increased bleeding risk. However, it remains unclear whether constipation is associated with an increased risk of incident bleeding complications in AF with HF. Here, we investigated the association between constipation requiring laxatives and major bleeding in AF and HF. We retrospectively analyzed the medical records of 370 consecutive patients hospitalized for AF and congestive HF. Constipation was defined as regularly taking laxatives or having at least two prescriptions for a ≥ 30-day supply of laxatives. Sixty patients experienced major bleeding events during a median follow-up of 318 days. The most common sites of bleeding were lower gastrointestinal (28%, 17/60), upper gastrointestinal (27%, 16/60), and intracranial (20%, 12/60). There were 33 (55%) patients with constipation in the bleeding group and 107 (35%) in the non-bleeding group (P = 0.004). Multivariate Cox regression analysis adjusted for HAS-BLED score, hemoglobin, and direct oral anticoagulant use showed that constipation (hazard ratio [HR] 1.85, 95% confidence interval [CI] 1.11–3.08; p = 0.019) was a significant risk factor for major bleeding. We found a significant association between constipation requiring laxatives and major bleeding in patients with AF and HF. These findings indicate the need for constipation prevention in these patients to avoid reliance on invasive defecation management, including laxatives.
Transanal endoscopic microsurgery versus radical resection for early-stage rectal cancer: a systematic review and meta- analysis
Authors: Li Wei , Xiang Xing Xing , Da Wang Hong , Cai Chen Jun , Cao Ying Hao , Liu Tao
Published: February 2023

Purpose

In the treatment of early-stage rectal cancer, a growing number of studies have shown that transanal endoscopic microsurgery is one of the alternatives to radical surgery adhering to total mesorectal excision that can reduce the incidence of adverse events without compromising treatment outcomes. The purpose of this meta-analysis is to compare the safety and treatment effect of transanal endoscopic microsurgery and radical surgery adhering to total mesorectal excision to provide a basis for clinical treatment selections.

Method

We searched the literatures of four major databases, PubMed, Embase, Web of science, and Cochrane Library, without limitation of time. The literatures included randomized controlled studies and cohort studies comparing two surgical procedures of transanal endoscopic microsurgery and radical surgery adhering to total mesorectal excision. Treatment effectiveness and safety results of transanal endoscopic microsurgery and radical surgery were extracted from the included literatures and statistically analyzed using RevMan5.4 and stata17.

Result

Ultimately, 13 papers were included in the study including 5 randomized controlled studies and 8 cohort studies. The results of the meta-analysis showed that the treatment effect and safety of both transanal endoscopic microsurgery and radical surgery in distant metastasis (RR, 0.59 (0.34, 1.02), P > 0.05), overall recurrence (RR, 1.49 (0.96, 2.31), P > 0.05), disease-specific-survival (RR, 0.74 (0.09, 1.57), P > 0.05), dehiscence of the sutureline or anastomosis leakage (RR, 0.57 (0.30, 1.06), P > 0.05), postoperative bleeding (RR, 0.47 (0.22, 0.99), P > 0.05), and pneumonia (RR, 0.37, (0.10, 1.40), P > 0.05) were not significantly different. However, they differ significantly in perioperative mortality (RR, 0.26 (0.07, 0.93, P < 0.05)), local recurrence (RR, 2.51 (1.53, 4.21), P < 0.05),_overall survival_ (RR, 0.88 (0.74, 1.00), P < 0.05), disease-free-survival (RR, 1.08 (0.97, 1.19), P < 0.05), temporary stoma (RR, 0.05 (0.01, 0.20), P < 0.05), permanent stoma (RR, 0.16 (0.08, 0.33), P < 0.05), postoperative complications (RR, 0.35 (0.21, 0.59), P < 0.05), rectal pain (RR, 1.47 (1.11, 1.95), P < 0.05), operation time (RR, −97.14 (−115.81, −78.47), P < 0.05), blood loss (RR, −315.52 (−472.47, −158.57), P < 0.05), and time of hospitalization (RR, −8.82 (−10.38, −7.26), P < 0.05).

Conclusion

Transanal endoscopic microsurgery seems to be one of the alternatives to radical surgery for early-stage rectal cancer, but more high-quality clinical studies are needed to provide a reliable basis.
FraMaDySc: dysphagia screening for patients after surgery for head and neck cancer
Authors: Hey Christiane , Goeze Almut , Sader Robert , Zaretsky Eugen
Published: February 2023

Purpose

Oropharyngeal dysphagia is one of the most common postoperative consequences in head and neck cancer patients. Above all, these patients often suffer from aspiration and limitations of oral intake. However, no reliable dysphagia screening is available for this target group. This study aimed to develop and validate a screening, FraMaDySc, based on a water swallow test (WST) for the identification of postsurgical patients with a risk of aspiration, limitations of oral intake, and, as their derivate, a relevant oropharyngeal dysphagia in general (OD) that constituted the main reference standard.

Methods

A total of 184 postsurgical head and neck cancer patients were tested with a WST. The patients were, on average, 62 years old and predominantly male (71%). After WST, they underwent Fiberoptic Endoscopic Evaluation of Swallowing (FEES®). FEES® results were dichotomized by Penetration aspiration scale and Functional oral intake scale. Patients with a “fail” result on one of these two scales were classified as having OD. Quality criteria of WST were quantified by means of cross-tabulation with FEES® results.

Results

OD was found in 65% of patients, aspiration in 44%, limitations of oral intake in 56%. WST delivered a “fail” result in 65% of patients. WST showed sensitivity 91% and specificity 88% for OD. Quality criteria for aspiration (sensitivity 64%, specificity 93%) and limitations of oral intake (sensitivity 80%, specificity 87%) were lower.

Conclusion

FraMaDySc is a standardized, quick, and valid WST and therefore an excellent screening tool for the identification of OD in postoperative head and neck cancer patients.
Self-assessment of olfactory function using the “Sniffin’ Sticks”
Authors: Mai Yiling , Klockow Marie , Haehner Antje , Hummel Thomas
Published: February 2023

Background

A precise and reliable test of the olfactory function is indispensable for the diagnosis of the olfactory disorder (OD). Despite of this, in a clinical context, often there is no place in daily routine for time-consuming procedures. This study aimed to examine if the assessment of olfactory function using the “Sniffin’ Sticks” is suitable for self-assessment.

Methods

Participants comprised 84 healthy control subjects (HC) and 37 OD patients. The “Sniffin’ Sticks” test battery consisting of odor threshold (T), discrimination (D) and identification (I) tests was used for self- and assisted assessments. To save time, we applied the 8-item wide step version of the T test and the 8-item D test, whereas the I task remained the same as the original version. The whole test included two sessions, with each session comprising a self-assessment part performed by the participants themselves, and an assisted-assessment part performed by the examiner.

Results

Sniffin’ Sticks self-assessment was efficient in distinguishing between self-reported HC subjects and OD patients (p’s < 0.01), and the scores did not differ significantly from the assisted-assessment (p’s > 0.05). In the self-administered I and TDI tests, there was a moderate to excellent test–retest reliability (ICC = 0.51–0.93, p’s < 0.01), and a strong to excellent correlation with the assisted assessment (r = 0.71–0.92, p’s < 0.01). However, the self-administered T and D tests only exhibited low to moderate test–retest reliability (ICC = 0.30–0.72, p’s < 0.05) and correlations with the assisted test (r = 0.31–0.62, p’s < 0.05).

Conclusions

The Identification self-test is appropriate to be solely applied, and is therefore an easy-to-use alternative for olfactory screening in a larger segment of patients. The whole “Sniffin’ Sticks” self-test also shows good measurement properties and is therefore a suitable backup in clinical practice, but improvement is needed due to the simplified D and T self-test.
Pontine autosomal dominant microangiopathy with leukoencephalopathy: Col4A1 gene variants in the original family and sporadic stroke
Authors: Roos Jessica , Müller Stefanie , Giese Anne , Appenzeller Silke , Ringelstein Erich Bernd , Fiehler Jens , Berger Klaus , Rolfs Arndt , Hagel Christian , Kuhlenbäumer Gregor
Published: February 2023

Background

(1) Description of clinical and cranial MRI features in the original Pontine Autosomal Dominant Microangiopathy with Leukoencephalopathy (PADMAL) family and correlation with the segregation analysis of the causative collagen 4A1 gene (COL4A1) variant. (2) Sequence analysis of the COL4A1 miRNA-binding site containing the causative variant in two independent cross-sectional samples of sporadic stroke patients.

Patients and methods

Sanger sequencing of the COL4A1 miRNA-binding site in the PADMAL family and 874 sporadic stroke patients.

Results

PADMAL shows adult-onset usually between 30 and 50 years of age with initial brainstem-related symptoms most commonly dysarthria, with progression to dementia and tetraparesis. Radiologically pontine lacunes are followed by supratentorial white matter involvement. Radiological onset may precede clinical symptoms. We found no variants in the COL4A1 miRNA-binding site of sporadic stroke patients.

Conclusion

Our results allow an early diagnosis of PADMAL based on cranial MRI, clinical signs, and confirmatory sequencing of the COL4A1 miRNA-29-binding site. COL4A1 miRNA-29-binding site variants do not contribute to a sizeable proportion of sporadic stroke.
Peripheral nervous system adverse events associated with immune checkpoint inhibitors
Authors: Rossi Simone , Gelsomino Francesco , Rinaldi Rita , Muccioli Lorenzo , Comito Francesca , Di Federico Alessandro , De Giglio Andrea , Lamberti Giuseppe , Andrini Elisa , Mollica Veronica , D’Angelo Roberto , Baccari Flavia , Zenesini Corrado , Madia Pierandrea , Raschi Emanuel , Cortelli Pietro , Ardizzoni Andrea , Guarino Maria
Published: February 2023

Background

Immune checkpoint inhibitors (ICIs) represent an effective cancer immunotherapy yet are associated with immune-related adverse events (irAEs). The aim of this study was to characterize irAEs involving the peripheral nervous system (PNS-irAEs) in a real-world cohort of ICI-treated patients.

Methods

Cancer patients treated with ICIs between January 2014 and March 2022 were included. Patients with PNS-irAEs were identified and divided into two groups: (1) cranial/peripheral neuropathies and (2) myasthenia gravis (MG) and/or myositis. Clinical characteristics and outcomes, measured with the modified Rankin Scale (mRS), were compared among the two groups.

Results

Among 920 ICI-treated patients, 20 patients (2.17%) developed a PNS-irAEs. The median latency from ICI exposure was 8.8 weeks and the median time from onset to clinical nadir was 3.5 weeks. Eleven patients developed a neuropathy: polyneuropathy (n = 4), cranial neuropathy (n = 3), small-fiber neuropathy (n = 3), brachial plexopathy (n = 1). Nine patients presented MG and/or myositis: concomitant MG and myositis (n = 6), isolated myositis (n = 2), exacerbation of MG (n = 1). Immunosuppressive treatment and/or ICI withdrawal determined a significant clinical improvement, expressed by a mRS reduction, in the neuropathy group (p = 0.004), but not in the MG/myositis group (p = 0.11). Overall, death due to irAEs occurred in four patients (20%), all with MG/myositis. Compared to patients with neuropathies, those with MG/myositis had a shorter latency onset (p = 0.036), developed more frequently concomitant non-neurologic irAEs (p = 0.028) and showed a higher mortality rate (p = 0.026).

Conclusions

In our large cohort of ICI-treated patients, 2.17% developed PNS-irAEs. Compared to ir-neuropathies, ir-MG/myositis tend to occur earlier from ICI exposure and present a worse response to treatment and a higher mortality.
Endoscopic vacuum therapy significantly improves clinical outcomes of anastomotic leakages after 2-stage, 3-stage, and transhiatal esophagectomies
Authors: Maier Jonas , Kandulski A. , Donlon N. E. , Werner J. M. , Mehrl A. , Müller M. , Doenecke A. , Schlitt H. J. , Hornung M. , Weiss A. R. R.
Published: February 2023

Background

Anastomotic leakages after esophagectomies continue to constitute significant morbidity and mortality. Intrathoracic anastomoses pose a high risk for mediastinitis, sepsis, and death, if a leak is not addressed timely and appropriately. However, there are no standardized treatment recommendations or algorithms as for how to treat these leakages.

Methods

The study included all patients at the University Hospital Regensburg, who developed an anastomotic leakage after esophagectomy with gastric pull-up reconstruction from 2007 to 2022. Patients receiving conventional treatment options for an anastomotic leakage (stents, drainage tubes, clips, etc.) were compared to patients receiving endoscopic vacuum-assisted closure (eVAC) therapy as their mainstay of treatment. Treatment failure was defined as cervical esophagostomy formation or death.

Results

In total, 37 patients developed an anastomotic leakage after esophagectomy with a gastric pull-up reconstruction. Twenty patients were included into the non-eVAC cohort, whereas 17 patients were treated with eVAC. Treatment failure was observed in 50% of patients (n = 10) in the non-eVAC cohort and in 6% of patients (n = 1) in the eVAC cohort (p < 0.05). The 90-day mortality in the non-eVAC cohort was 15% (n = 3) compared to 6% (n = 1) in the eVAC cohort. Cervical esophagostomy formation was required in 40% of cases (n = 8) in the non-eVAC cohort, whereas no patient in the eVAC cohort underwent cervical esophagostomy formation.

Conclusion

eVAC therapy for leaking esophagogastric anastomoses appears to be superior to other treatment strategies as it significantly reduces morbidity and mortality. Therefore, we suggest eVAC as an essential component in the treatment algorithm for anastomotic leakages following esophagectomies, especially in patients with intrathoracic anastomoses.
Aerobically trained older adults show impaired resting, but preserved exercise-induced circulating progenitor cell count, which was not improved by sprint interval training
Authors: Yasar Zerbu , Ross Mark D. , Gaffney Christopher J. , Postlethwaite Ruth D. , Wilson Russell , Hayes Lawrence D.
Published: February 2023

Older adults exhibit a reduced number and function of CD34 + circulating progenitor cells (CPC), a known risk factor for cardiovascular disease. Exercise promotes the mobilisation of CPCs from bone marrow, so whether ageing per se or physical inactivity in older age reduces CPCs is unknown. Thus, this study examined the effect of age on resting and exercise-induced changes in CPCs in aerobically trained adults and the effect of 8 weeks of sprint interval training (SIT) on resting and exercise-induced CPCs in older adults. Twelve young (22–34 years) and nine older (63–70 years) adults participated in the study. Blood was sampled pre and immediately post a graded exercise test to exhaustion in both groups. Older participants repeated the process after 8 weeks of SIT (3 × 20 s ‘all-out’ sprints, 2 × a week). Total CPCs (CD34+) and endothelial progenitor cells (EPCs: CD34+KDR+) were determined by flow cytometry. Older adults exhibited lower basal total CD34+ CPCs (828 ± 314 vs. 1186 ± 272 cells·mL−1, p = 0.0149) and CD34+KDR+ EPCs (177 ± 128 vs. 335 ± 92 cells·mL−1, p = 0.007) than younger adults. The maximal exercise test increased CPCs in young (CD34+: p = 0.004; CD34+KDR+: p = 0.017) and older adults (CD34+: p < 0.001; CD34+KDR+: p = 0.008), without difference between groups (p = 0.211). SIT did not alter resting or exercise-induced changes in CPCs in the older cohort (p > 0.232). This study suggests age per se does not impair exercise-induced CPC counts, but does lower resting CPC counts.
Visuospatial working memory abilities in children analyzed by the bricks game task (BGT)
Authors: D’Aurizio G. , Di Pompeo I. , Passarello N. , Troisi Lopez E. , Sorrentino P. , Curcio G. , Mandolesi L.
Published: February 2023

The study of the development of visuospatial memory processes is useful for devising personalized educational interventions as well as for understanding the changes in cognitive functioning in an era characterized by technological progress. The present research is aimed at investigating spatial working memory ability in children that attended the first three years of primary school by means of the Brick Game Task (BGT), a novel visuospatial working memory test. BGT is a small-scale ecological test inspired by behavioral walking tasks with nine white bricks in different spatial configurations as well as to Corsi Block-Tapping test.

228 Italian children (121 F; mean age: 7.22 ± 1.18) were assigned to three groups based on the primary school class attended: Group 1 (N = 85; 40 F; mean age 6.18 ± .5), Group 2 (N = 61; 36 F; mean age 7.2 ± .83), and Group 3 (N = 82; 44 F; mean age 8.32 ± .94). All participants were asked to complete the Digit Span test, the Corsi Block-Tapping test, and to explore the three spatial configurations of the BGT with the form of Matrix, M-BGT, Cluster, CL-BGT, Cross, CR-BGT.

MANOVA revealed a main significant effect for Group (F12,434 = 15.06; p < .0001) indicating that the group of older obtained a better global executive performance than 1 and 2 groups. Multiple linear regression indicated that Corsi Block-Tapping test performance and Age significantly predicted the M-BGT score. Moreover, Corsi Block-Tapping test and Digit Span significantly predicted the CL-BGT performance, showing how a higher score results in a better CL- BGT performance. Finally, Corsi Block-Tapping test, Digit Span, and Age were positively associated with the CR- BGT performance. The present findings evidenced that novel BGT is a sensible visuospatial working memory task suggesting thus its use to assess the children’s executive performance in ecological way. These results open to the development of personalized educational interventions.
Colorectal adenosquamous carcinoma: genomic profiling of a rare histotype of colorectal cancer
Authors: Angerilli Valentina , Parente Paola , Businello Gianluca , Vanoli Alessandro , Paudice Michele , Perrone Giovanni , Munari Giada , Govoni Ilaria , Neri Giuseppe , Rebellato Elena , Parrella Paola , Grillo Federica , Mastracci Luca , Fassan Matteo
Published: February 2023

Colorectal adenosquamous carcinoma (ASC) is exceedingly rare, comprising less than 0.1% of all colorectal malignancies, and is characterized by an aggressive disease course, with a higher metastatic rate and worse outcome than conventional colorectal adenocarcinoma. A comprehensive molecular profile of this group of neoplasms is still lacking. A total of 22 cases of colorectal ASCs (with 22 primary lesions and 7 metastases matched with 4 primaries) were subject to NGS targeting 67 cancer-related genes (VariantPlex solid tumor; Archer). Mismatch repair (MMR), p53, and V600EBRAF status were also investigated by immunohistochemistry. In 28 of 29 (96.6%) ASC samples, at least one single-nucleotide variant (SNV) or copy number variation (CNV) was detected. Among the 22 primary tumors, the most frequently mutated genes were TP53 (59.1%), APC (40.9%), KRAS (27.3%), BRAF (13.6%), and GNAS (9.1%). Only 1/22 (4.5%) primary ASC was MMR-deficient (MMRd) and harbored a BRAF mutation. Limited differences in SNVs were observed between primary and metastatic diseases. This study sheds light on the molecular landscape of colorectal ASCs. According to our data, the genomic profile of colorectal ASC is similar to that of conventional colorectal carcinoma, with significant druggable genetic alterations. Further studies are required to understand the more aggressive clinical behavior of this neoplasm.
Predicting the clinical trajectory of feeding and swallowing abilities in CHARGE syndrome
Authors: Onesimo R. , Sforza E. , Giorgio V. , Rigante D. , Kuczynska E. , Leoni C. , Proli F. , Agazzi C. , Limongelli D. , Cerchiari A. , Tartaglia M. , Zampino G.
Published: February 2023

To date, the feeding and oral-motor abilities of patients with CHARGE syndrome (CS) have not been longitudinally assessed. This study aims to investigate the level of these abilities at different ages and evaluate how they evolve during growth. We retrospectively analysed oral-motor features of 16 patients with molecularly confirmed CS (age range 4–21 years old; mean 11 years; SD 6 years; median 10 years). Nearly 100% of CS new-borns had weak sucking at birth, and half of them demonstrated poor coordination between breathing and swallowing. Over time, the percentages of children with tube feeding dependence (60% at birth) faced a slow but steady decrease (from 33% at 6 months, 25% at 12 months, to 13% at school age) in tandem with the decreasing risk of aspiration. The ability of eating foods requiring chewing was achieved at school age, after the acquisition of an adequate oral sensory processing. A mature chewing pattern with a variety of food textures was not achieved by more than half of patients, including those requiring artificial enteral nutrition. Most patients started prolonged oral-motor treatments with speech language therapists in early childhood.

Conclusions: Although feeding and swallowing disorders are constant features in CS patients, a slow and gradual development of feeding abilities occurs in most cases. Rehabilitation plays a key role in overcoming structural and functional difficulties and attaining appropriate eating skills.

What is Known:• Feeding problems and swallowing dysfunction have been noted in CHARGE syndrome.• The involvement of multiple factors, including structural problems in the mouth, throat, or esophagus, and neurological impairment, make feeding a complicated task in CHARGE individuals.

What is New:• Dysphagia gradually improves in most CHARGE children over time, though with a wide interindividual variability.• The percentages of children with tube feeding dependence decrease over time from 60% at birth to 33% at 6 months and 13% at school age.
Beyond IBD: the genetics of other early-onset diarrhoeal disorders
Authors: Stallard Lorraine , Siddiqui Iram , Muise Aleixo
Published: February 2023

Diarrhoeal disorders in childhood extend beyond the inflammatory bowel diseases. Persistent and severe forms of diarrhoea can occur from birth and are associated with significant morbidity and mortality. These disorders can affect not only the gastrointestinal tract but frequently have extraintestinal manifestations, immunodeficiencies and endocrinopathies. Genomic analysis has advanced our understanding of these conditions and has revealed precision-based treatment options such as potentially curative haematopoietic stem cell transplant. Although many new mutations have been discovered, there is frequently no clear genotype–phenotype correlation. The functional effects of gene mutations can be studied in model systems such as patient-derived organoids. This allows us to further characterise these disorders and advance our understanding of the pathophysiology of the intestinal mucosa. In this review, we will provide an up to date overview of genes involved in diarrhoeal disorders of early onset, particularly focussing on the more recently described gene defects associated with protein loosing enteropathy.
Time-optimal construction of overlay networks
Authors: Götte Thorsten , Hinnenthal Kristian , Scheideler Christian , Werthmann Julian
Published: February 2023

This article shows how to construct an overlay network of constant degree and diameter$$O(\log n)$$O(logn)in$$O(\log n)$$O(logn)time starting from an arbitrary weakly connected graph. We assume a synchronous communication network in which nodes can send messages to nodes they know the identifier of, and new connections can be established by sending node identifiers. Suppose the initial network’s graph is weakly connected and has constant degree. In that case, our algorithm constructs the desired topology with each node sending and receiving only$$O(\log n)$$O(logn)messages in each round in$$O(\log n)$$O(logn)time w.h.p., which beats the currently best$$O(\log ^{3/2} n)$$O(log3/2n)time algorithm of Götte et al. (International colloquium on structural information and communication complexity (SIROCCO), Springer, 2019). Since the problem cannot be solved faster than by using pointer jumping for$$O(\log n)$$O(logn)rounds (which would even require each node to communicate$$\Omega (n)$$Ω(n)bits), our algorithm is asymptotically optimal. We achieve this speedup by using short random walks to repeatedly establish random connections between the nodes that quickly reduce the conductance of the graph using an observation of Kwok and Lau (Approximation, randomization, and combinatorial optimization. Algorithms and techniques (APPROX/RANDOM 2014), Schloss Dagstuhl-Leibniz-Zentrum fuer Informatik, 2014). Additionally, we show how our algorithm can be used to efficiently solve graph problems inhybrid networks(Augustine et al. in Proceedings of the fourteenth annual ACM-SIAM symposium on discrete algorithms, SIAM, 2020). Motivated by the idea that nodes possess two different modes of communication, we assume that communication of theinitialedges is unrestricted, whereas only polylogarithmically many messages can be sent over edges that have been established throughout an algorithm’s execution. For an (undirected) graphGwith arbitrary degree, we show how to compute connected components, a spanning tree, and biconnected components in$$O(\log n)$$O(logn)time w.h.p. Furthermore, we show how to compute an MIS in$$O(\log d + \log \log n)$$O(logd+loglogn)time w.h.p., wheredis the initial degree ofG.
Combinatorics and Real Lifts of Bitangents to Tropical Quartic Curves
Authors: Cueto Maria Angelica , Markwig Hannah
Published: February 2023

Smooth algebraic plane quartics over algebraically closed fields of characteristic different than two have 28 bitangent lines. Their tropical counterparts often have infinitely many bitangents. They are grouped into seven equivalence classes, one for each linear system associated to an effective tropical theta characteristic on the tropical quartic. We show such classes determine tropically convex sets and provide a complete combinatorial classification of such objects into 41 types (up to symmetry). The occurrence of a given class is determined by both the combinatorial type and the metric structure of the input tropical plane quartic. We use this result to provide explicit sign-rules to obtain real lifts for each tropical bitangent class, and confirm that each one has either zero or exactly four real lifts, as previously conjectured by Len and the second author. Furthermore, such real lifts are always totally-real.
Efficient probabilistic prediction of tsunami inundation considering random tsunami sources and the failure probability of seawalls
Authors: Fukutani Yo , Yasuda Tomohiro , Yamanaka Ryoichi
Published: February 2023

Probabilistic tsunami inundation assessment ordinarily requires many inundation simulations that consider various uncertainties; thus, the computational cost is very high. In recent years, active research has been conducted to reduce the computational cost. In this study, the number of random tsunami sources was reduced to 20% of the original number by applying proper orthogonal decomposition (POD) to tsunami inundation depth distributions obtained from random tsunami sources. Additionally, the failure degree of seawalls was stochastically assessed, and its impact was incorporated into the evaluation model for tsunami inundation hazards because this factor has a significant impact on the tsunami inundation depth assessment for land areas. Although the randomness of the slip distribution in tsunami sources has been studied extensively in the past, the idea of simultaneously modelling the failure degree of seawalls is a novel feature of this study. Finally, tsunami inundation distribution maps were developed to represent the probability of occurrence of different inundation depths for the next 50 years and 10 years by using a number of tsunami inundation distributions that consider the randomness of the tsunami sources and the failure probability of the seawalls.
Statistical arbitrage in the stock markets by the means of multiple time horizons clustering
Authors: Gatta Federico , Iorio Carmela , Chiaro Diletta , Giampaolo Fabio , Cuomo Salvatore
Published: February 2023

Nowadays, statistical arbitrage is one of the most attractive fields of study for researchers, and its applications are widely used also in the financial industry. In this work, we propose a new approach for statistical arbitrage based on clustering stocks according to their exposition on common risk factors. A linear multifactor model is exploited as theoretical background. The risk factors of such a model are extracted via Principal Component Analysis by looking at different time granularity. Furthermore, they are standardized to be handled by a feature selection technique, namely the Adaptive Lasso, whose aim is to find the factors that strongly drive each stock’s return. The assets are then clustered by using the information provided by the feature selection, and their exposition on each factor is deleted to obtain the statistical arbitrage. Finally, the Sequential Least SQuares Programming is used to determine the optimal weights to construct the portfolio. The proposed methodology is tested on the Italian, German, American, Japanese, Brazilian, and Indian Stock Markets. Its performances, evaluated through a Cross-Validation approach, are compared with three benchmarks to assess the robustness of our strategy.
Thermochronological constraints on the post-Variscan exhumation history of the southeastern Bohemian Massif (Waldviertel and Weinsberg Forest, Austria): palaeogeographic and geomorphologic implications
Authors: Hejl Ewald , Heberer Bianca , Salcher Bernhard , Sekyra Gert , Van den haute Peter , Leichmann Jaromír
Published: February 2023

Resolving the Mesozoic and Cenozoic palaeogeography and geomorphologic development of outcropping Variscan basement is a pre-condition for the understanding of central European geodynamics. For our study, we have applied apatite fission-track (AFT) and apatite (U–Th)/He (AHe) thermochronology to surface rocks of the southeastern Bohemian Massif. 46 samples were examined by the AFT method. Additional AHe dating was applied to 8 of them. The AFT ages range from 251 ± 46 to 60.2 ± 4.8 Ma. AHe ages range from 25 to 525 Ma with rather high intra-sample scatter. On a regional scale, the AFT ages generally decrease from mainly late Variscan in the NE to Late Cretaceous and Paleocene in the SW. This regional age asymmetry relative to the NW–SE trending watershed of the Weinsberg Forest is neither compatible with regional uplift of a single block nor with large-scale lithospheric updoming. The lack of age breaks along late Variscan faults demonstrates that strong vertical offset cannot have occurred in Cretaceous and Cenozoic times. Inverse modeling of thermochronological data indicates regional Early Cretaceous cooling and subsequent reheating during the Late Cretaceous. Rocks of the present-day surface were heated up to a temperature of ca. 80 °C without full reset of the AFT system. This thermal history is compatible with the existence of a large mainland in Early Cretaceous times and a subsequent sedimentary reburial until the Campanian on the order of up to 1 km overburden. Parts of the exhumed weathering basal relief to the N and NE of the Weinsberg Forest are inherited as ‘sealed relief’ from Middle Cretaceous time. The observed regional asymmetry of AFT data is best explained by the development of a continental escarpment adjacent to the North Penninic Ocean in latest Cretaceous to Paleogene times. A final episode of accelerated cooling after ca. 20 Ma, as indicated by thermochronological modeling, is tentatively ascribed to either collisional coupling of the Alpine-Carpathian nappe pile with its northern foreland or to East-Alpine slab detachment.
A Limit Equilibrium Model to Simulate Time-Dependent Pillar Scaling in Hard Rock Bord and Pillar Mines
Authors: Wessels D. G. , Malan D. F.
Published: February 2023

This study investigated the use of a limit equilibrium model to simulate the time-dependent scaling of hard rock pillars. In the manganese bord and pillar mines in South Africa, extensive scaling is observed for pillars characterised by a high joint density. It appears that the scaling occurs in a time-dependent fashion. Evidence for this is the ongoing deterioration of pillars in old areas, even after the pillars are reinforced with thin spray-on liners. Monitoring of selected pillars were conducted in an attempt to quantify the rate of time-dependent scaling. Contrary to expectations, almost no additional scaling was recorded for the pillars during a 3-month monitoring period. The scaling distance for pillars of different ages could be measured and it seems as if most of the scaling occurred soon after the pillars are formed. Only a limited amount of additional time-dependent scaling seems to occur after this. Numerical simulations of the time-dependent scaling were conducted using a displacement discontinuity code and a limit equilibrium constitutive model. The postulated exponential decay of the failed rock mass strength at the edges of the pillars resulted in simulated behaviour that is qualitatively similar to the underground observations. The results from this study are encouraging and the method can be used to investigate the long-term stability of bord and pillar excavations. Further work is required to improve on the calibration of the model and to better quantify the rate of scaling of the underground pillars.