bluuebunny/crossref_metadata_2025 huggingface dataset:
60 Use of environmental monitoring to inform biosecurity compliance in a swine health challenge
Authors: Harrison Olivia , Stewart Savannah , Vier Carine , Gebhardt Jordan , Jones Cassandra K , Woodworth Jason C , Paulk Chad B , Dritz Steve S
Published: November 2020

As endemic and foreign diseases threaten the U.S. swine herd, producers are scrutinizing on-farm biosecurity practices. Environmental monitoring may help inform biosecurity compliance. Unfortunately, the Kansas State Swine Teaching and Research Center suffered an outbreak of porcine epidemic diarrhea virus (PEDV) in March 2019. The objective of this research was to use environmental monitoring to evaluate biosecurity risk on a farm experiencing a viral outbreak. In a completely randomized design, environmental swabs were collected after barn sanitation on 14, 28, and 42-days after initial PEDV diagnosis from four zones: 1) pig contact surfaces within barns, 2) non-pig contact surfaces within barns, 3) outside barns and inside offices, and 4) transition areas. Swabs were analyzed for PEDV, rotavirus, and Enterobacteriaceae at a veterinary diagnostic laboratory. Data were analyzed using the GLIMMIX procedures of SAS, v 9.4 (Cary, NC). There was a significant time × zone interaction for PEDV (P < 0.0001), where swabs from non-pig contact areas within barns on d 28 (Zone 2) had greater (P < 0.05) levels of PEDV than pig-contact areas (Zone 1) on d 14, 28, or 42. No other interactions existed, and they were therefore removed from the statistical model. Time did not affect (P > 0.05) any measured response criteria. No PEDV was detected (45.0 Ct) from Zone 1 due to recent sanitation, but viral presence was highest (P < 0.05) in non-pig contact areas inside barns and was tracked outside of barns and through transition zones (32.9, 34.3, and 38.5 Ct for zones 2, 3, and 4, respectively). There was no detected difference (P > 0.05) for rotavirus or Enterobacteriaceae in time or zone. In conclusion, producers experiencing a PEDV outbreak may successfully decontaminate pig-contact surfaces, but still have risk throughout the farm. Biosecurity practices should support preventing cross-contamination from other contaminated areas.
PSV-7 Effects of drying and oxygenation of piglets at birth on rectal temperatures in the early post-natal period
Authors: Vande Pol Katherine , Cooper Naomi , Tolosa Andres , Ellis Michael , Gates Richard , Xiong Yijie , Shull Caleb M , Brown Katie , da Silva Stephan
Published: November 2020

Piglets often experience hypothermia early after birth. Previous research has suggested that drying piglets and administration of oxygen (a potential treatment for asphyxiation) at birth may increase post-natal rectal temperatures. The objective of this study was to determine the effects of drying and administering oxygen at birth on piglet rectal temperature over the first 24 h after birth. The study, conducted at a commercial facility, used a CRD with 42 sows/litters randomly allotted at start of farrowing to 3 treatments (applied at birth): Control (no drying or oxygenation); Dried (using a cellulose-based desiccant); Dried+Oxygen [dried and placed in a chamber (40% oxygen) for 20 min]. At birth, piglets were weighed and uniquely identified. Rectal temperature was measured at 0, 20, 30, 45, 60, 120, and 1440 min after birth. Data were analyzed using PROC MIXED of SAS. Litter was the experimental unit; piglet was a subsample of litter. The statistical model included effects of treatment, time of measurement, and the interaction. Both the Dried and Dried+Oxygen treatments had greater (P < 0.05) rectal temperatures than the Control between 20 and 120 min. However, the Dried+Oxygen treatment had lower (P < 0.05) rectal temperatures than the Dried treatment between 20 and 60 minutes. Temperatures at 1440 min were lower (P < 0.05) for the Dried+Oxygen than the other treatments; however, differences were small. In conclusion, drying piglets at birth increased rectal temperatures over the first 2 h after birth. The combination of drying piglets at birth and placement in an oxygen chamber for 20 min was less effective at moderating post-natal temperature changes than drying alone. Further research on piglet oxygenation is necessary to understand the reason for these reduced temperatures, and whether this treatment affects pre-weaning mortality. This research was funded by the National Pork Board.
PSV-10 The use of oleic acid during heat stress on lameness and joint health
Authors: Dotson Morgan L , Schnurbusch Teagan R , Wiegand Bryon R , Anderson Nichole C
Published: November 2020

Though heat stress is becoming increasingly common in pig production, efforts are ongoing to mitigate the negative effects of heat stress on feed consumption. During a period of heat stress, 40 pigs (87.4 ± 0.86 kg) were fed oleic acid (0%, 2%, 4%, 6% and 8%, n = 8 per diet) to improve growth rate. With the rising concern for welfare issues in swine production, we sought to measure how the diet would affect lameness in growing pigs. Therefore, the objectives of this study were to 1) use thermal imaging as a noninvasive measure to detect lameness and 2) to measure effects of oleic acid on the development of osteochondritic lesions. For 6 weeks during the growing phase, visual observations for lameness were conducted by a single trained observer. Additionally, thermal imaging was performed on the lateral aspect of each leg to include the stifle or elbow joint to the foot. At 120.6 ± 1.11 kg, pigs were necropsied and evaluated for joint health by measurement of the number of lesions, total size of lesions, and cartilage score on the proximal humerus, radius/ulna, and tibia/fibula, and the distal aspect of the humerus, radius/ulna, femur, and tibia/fibula. Scoring of the cartilage was based on a 0 to 4 scale, where 0 represents no lesions, and 4 represents severely damaged cartilage. No treatment effects were observed (P > 0.10), however overall lameness scores increased as pigs aged (P < 0.01). Only pigs in the control treatment (0% oleic acid) had an overall increase in temperatures of the right and left knee (P < 0.01). No observable differences occurred in cartilage scores, total lesion size, or number of lesions (P > 0.10) regardless of joint or treatment. Further research is needed to measure if oleic acid in the diet could improve joint health.
PSV-19 Pasture history and supplementation affects parasite status of naïve lambs in grazing systems
Authors: Campbell Braden J , McCutcheon Jefferson S , Marsh Antoinette E , Fluharty Francis L , Parker Anthony J
Published: November 2020

Developing alternative parasite management strategies is critical as anthelmintic resistance continues to be a global concern in small ruminant grazing operations. Converting cropland to pastureland may serve as one strategy to develop parasite free pasture, thus reducing the need for anthelmintic treatment. The objective of this study was to evaluate the impact of pasture contamination with gastrointestinal parasites and protein supplementation on the mitigation of parasitic infection in grazing lambs. We hypothesized that lambs grazing on newly established pasture (clean) without supplementation would perform similarly to those lambs grazing on permanent sheep pasture (infected) provided supplementation. A total of 96, 60-day old lambs (30 kg ± 4.0 kg), were randomly assigned to one of four treatments: 1) clean pasture without supplementation (CN); 2) clean pasture with supplementation (CS); 3) infected pasture without supplementation (IN); and 4) infected pasture with supplementation (IS). Packed cell volume (PCV) and fecal egg counts (FEC) were monitored biweekly. Data were analyzed using PROC MIXED in SAS. Based upon fecal egg fluorescence, 66% of the fluoresced eggs in the mixed infection population were Haemonchus contortus. There was a pasture × supplementation × day effect for PCV in that CN lambs had a greater PCV than IS lambs on days 28, 42, 56, and 70 (P < 0.0001). However, on days 98 and 112, IS lambs had a greater PCV than CN lambs (P < 0.001). In addition, there was a pasture × supplementation × day effect for FEC such that IS lambs demonstrated a greater FEC over time from day 28 to day 112 compared to CN lambs (P < 0.001). Offering supplemental protein to lambs grazing pastures contaminated with gastrointestinal parasites may help lambs cope with a heavy parasite burden. Therefore, developing additional management strategies will be key in reducing the future use of anthelmintics.
PSVII-9 Post transcriptional modifications may lead to changes in newborn goats’ skeletal muscle proteome as a consequence of maternal feed restriction at different stages of gestation
Authors: Costa Thaís , Mendes Tiago , Moura Felipe , Souza Ranyeri , Fontes Marta , Lopes Mariana , Serão Nick , Silva Fabyano , Gionbelli Mateus P , Duarte Marcio
Published: November 2020

We aimed to investigate the impact of maternal feed restriction at different stages of gestation on proteomic profile in the skeletal muscle of newborn goats. A total of 14 pregnant dams were randomly divided into one of the follow dietary treatments: Animals fed at 50% of maintenance requirement from 8-84 d of gestation and then fed at 100% maintenance requirement from day 85 of gestation to parturition (RM, n = 6), and animals fed at 100% of maintenance requirement from 8-84 d of gestation and then fed at 50% maintenance requirement from day 85 of gestation to parturition (MR, n = 8). Longissimus muscle was sampled from male newborn goats and submitted to sarcoplasmic protein extraction and liquid chromatography coupled to mass spectrometry (LC-MS) analysis. The raw data were processed with MaxQuant (1.6.3.3) software with parameters set to default values. Label-free quantification (LFQ) was added and only protein ratios calculated from at least two unique peptides were considered. Our data showed 3 differentially expressed proteins down-regulated in RM (q-value < 0.05). Additionally, we observed proteins present exclusively in each treatment (RM= 137 proteins; MR= 41 proteins). The overall enriched pathways in RM newborn goats are associated with glycolysis (PKM), NADPH synthesis (PGD), lipid oxidation (ECHS1, ACAT1) and citrate cycle (ACO1, OGDH). While the overall enriched pathways in MR newborn goats are associated with glycolysis/gluconeogenesis (GAPDH, ENO) and citrate cycle (PDHA, IDH3A). In addition, correlation analysis between shotgun proteomics and RNAseq data from the same samples showed that there were no relationships between proteins and transcripts observed. These results indicate that maternal feed restriction during different stages of gestation alters enzymes and protein domains abundance associated with nucleotide metabolism in the skeletal muscle of newborn goats. Moreover, the lack of correlation between protein-transcript suggests the importance of post-transcriptional regulation of skeletal muscle metabolism as a consequence of maternal feed restriction at different stages of gestation.
PSVII-11 Utilizing digital images to evaluate accuracy and repeatability of body condition score in cull sows
Authors: Taylor William E , Cassady Christopher J , Lonergan Steven M , Peyer Ben D , Stalder Kenneth J
Published: November 2020

The objective of this study evaluated the accuracy and repeatability when determining cull sow body condition scores (BCS) evaluated by scorers utilizing digital images. Participants (n=6) were selected based on previous BCS scoring on live sows. Group standards were established utilizing the scores from two participants with extensive experience BCS sows. Other scorers were not provided training before they scored images and no “scorer calibration” activity occurred to ensure that each scorer was observing similar traits when applying BCS. Two separate groups of sows were scored and video images for each sow were collected and stored for evaluation. The images were recorded as a convenience sample from a cooperating sow abattoir. The cull sow video images were assigned a BCS using an 8-point scale (1,1+,2-,2,3,4,5,NS). Scores from the lower portion of the scale were classified (+ and -) to provide a more specific BCS difference evaluation among sows that were thin. When scorers evaluated the sows (n=165) from the first group, they scored the BC for each sow. Additionally, a random group of sows (n=40) images were selected to be scored a second time by the same scorer. Repeatability (inter- and intra- scorer), individual bias, group bias, and group deviation from standard’s mean BCS were calculated. Data from the first sow group showed that relative to the standard’s mean scores, participants overestimated BCS by .41 (P=0.0001). A similarly selected second set of cull sow images obtained from the same abattoir was recorded. The second group included more sow images (n=220) and a greater number images that were scored a second time(n=55) in order to calculate the repeatability values. The same measures described for group 1 were calculated. After data analysis from the second sow group, it was again noted that participants overestimated BCS by 0.1 (P=0.0009).
PSII-17 Comparison of corn ethanol co-products from Brazil and USA apparent total tract digestibility of nutrients in pigs
Authors: Paula Vinicius R C , Milani Natália C , Azevedo Cândida P F , Souza Leury J , Sedano Anderson A , Mike Brian , Shurson Gerald C , Ruiz Urbano S
Published: November 2020

The objective of this study was to determine the apparent total tract digestibility (ATTD) of DM, GE, EE, NDF, ADF, and CP, the metabolizability of energy, of two high protein (HP) corn distillers dried grain (DDG) and of two corn DDG with solubles (DDGS), from Brazil and USA, and their respective digestible and metabolizable energy content in growing pigs. Fifty crossbred barrows (32.4±6.9kg) were distributed in a randomized block design, with 10 replicates, and received a 96.7 % corn basal diet (BD) or four diets with 40% of each test ingredient: Brazilian HP DDG (BHP); USA HP DDG (UHP); Brazilian DDGS (BDG) and USA DDGS (UDG), substituting for corn. Animals were fed at 2.8 x maintenance (110 kcal of DE per kg of BW0.75) for 7 d adaptation and 4 d of total feces and urine collection. The ATTD of nutrients and energy were calculated by difference and the results were submitted to ANOVA and Tukey’s test (P< 0.05). The BHP and UHP samples contained (as is basis) 92.3 – 89.6% DM, 2.8 – 3.5% ash, 42.9 – 34.9% CP, 10.3 – 7.3% EE, 32.7 – 47.5% NDF, 13.3 – 17.5% ADF, 5.30 – 4.90 Mcal/kg, respectively. The BDG and UDG samples contained (as is basis) 86.1 – 86.1% DM, 4.8 – 4.9% ash, 13.9 – 25.8% CP, 9.0 – 6.4% EE, 40.8 – 40.2% NDF, 13.3 – 14.3% ADF, 4.51 – 4.53 Mcal/kg, respectively. The ATTD of DM and GE and the digestible and metabolizable energy contents of BHP were greater (P< 0.05) than those verified in UHP. The ATTD of DM, GE, NDF, ADF, digestible and metabolizable energy values of BDG were lower (P < 0.05) than those verified in UDG. In conclusion, the BHP presented higher digestible values to almost all parameters evaluated, however, all ingredients presented acceptable digestible contents, indicating they may be used in pigs feeding.
PSII-21 The Effect of Feeding a LucraFit® Feed Nursery Program without Lactose on Piglet Performance
Authors: Crowder Stacie , Weeden Terry , Hankins Clayton , Meyer Terry , de Rodas Brenda
Published: November 2020

The objective of this study was to evaluate the effect of feeding LucraFit® Feed nursery program without lactose on piglet performance. A total of 440 weanling pigs, initial weight of 5.76 ± 0.23 kg., were randomly assigned to either Control or LucraFit® Feed treatments with 11 replicates per treatment and 20 pigs per pen. Pigs were weighed at day 0, 8, 15, 21, 29, and 43 post-weaning with corresponding ADG, ADFI, and G:F calculated for each time period. Data was analyzed using the Mixed procedure of SAS. Pigs were fed a three-phase nursery program with 2.27, 5.44, and 13.19 kg/pig for phases 1-3 respectively. Control diets contained 12.5% lactose in phase 1, 5% lactose in phase 2, and 0% lactose in phase 3. LucraFit® Feed diets contained 0% lactose in all phases. LucraFit® Feed was added at 2.5% and 1.25% in phase 1 and 2 respectively. During d0 to 8, there was no significant difference in growth measurements. During d8 to 15, pigs fed LucraFit® Feed treatment had increased (P=0.039) ADG (0.296 vs 0.266 kg/d) and improved (P=0.035) G:F ratio (0.977 vs 0.923). During d15 to 21, ADG was not significantly different between treatments, however ADFI was tended to increase (P=0.061) with the LucraFit® Feed treatment (0.526 vs 0.497 kg/d) resulting in decreased (P=0.047) G:F ratio (0.783 vs 0.824). Through d21 to 43, LucraFit® Feed tended to improve (P=0.082) G:F ratio (0.676 vs 0.660). Overall (d0 to 43) G:F ratio tended to improve (P=0.082) with the LucraFit® Feed treatment (0.723 vs 0.709). No significant differences were observed in pig removals between treatments. Final (d 43) body weight was not significantly different between treatments. These results suggest that pigs can be started on a LucraFit® Feed nursery program with 0% lactose without having a negative impact on nursery performance.
PSII-22 Trends in Mycotoxin Contamination in United States Corn
Authors: Hendel Erika , Ramirez Shelby , Gott Paige , Raj Murugesan G , Hofstetter Ursula
Published: November 2020

Mycotoxins are harmful secondary fungal metabolites and are of key concern to food and feed safety globally. These toxins are detrimental to animal health and can compromise animal performance even at low levels. Classic signs such as decreased feed intake and vomiting used as indicators for exposure overlook other costs of mycotoxicosis, including increasing the frequency and severity of disease via immune suppression, inciting inflammation, and modulating the gastrointestinal environment. This survey examines initial samples of the 2019 crop with previous year trends. New crop corn samples were submitted from September 2019 and consisted of corn (46%), corn silage (50%), and corn byproduct (4%). Samples were analyzed utilizing the liquid chromatography and tandem mass spectrometry (LC-MS/MS) method for six major mycotoxin groups: aflatoxins (Afla), type A trichothecenes (A-Trich), type B trichothecenes (B-Trich), fumonisins (FUM), zearalenone (ZEN), and ochratoxin-A (OTA). Data are presented for major mycotoxin classes in Table 1. Fewer samples are available thus far compared to the fall of 2018 (50 samples in 2019 vs. 135 samples in 2018), thus risk profile of this crop year is likely to change as the sample pool expands. Co-occurrence (≥ 2 mycotoxins) has decreased compared to 2018. The prevalence of B-Trich decreased compared with previous years, but levels are similar to 2018. Prevalence and levels of ZEN decreased from 2018, and are similar to 2017, while FUM is similar in prevalence to 2018, but average ppb numerically increased. As of yet, no Alfa has been detected; however, corn stored with higher moisture content has increased the risk for storage toxins. Mycotoxin risk of this harvest season is still coming into focus as harvest delays have affected sample submission. Due to continued risk of multi-mycotoxin contamination, multiple mitigation strategies are needed beyond just adsorption, including biotransformation, support of the immune system and liver function.
PSIX-3 Effect of a combination of brown, green and red seaweeds on performance of nursery and growing-finishing pigs, and on carcass characteristics
Authors: Manu Hayford , Huang Qiang , Pangeni Devi , Owusu-Asiedu Augustine , Sands Jason , Baidoo Samuel Kofi
Published: November 2020

The objective of the study was to investigate the effect of a proprietary combination of brown, green and red seaweeds OceanFeedTM Swine (OFS) as a replacement of in-feed antibiotic growth promotors (AGP) or pharmacological levels of zinc oxide on performance of nursery and growing finishing pigs and carcass characteristics. Three hundred and twenty crossbred piglets (5.88 ± 0.26 kg) were blocked by BW and randomly allotted to 1 or 4 dietary treatments of low ZnO and no AGP but formulated to meet NRC (2012) nutrient requirements (Negative control (NC, T1)), NC + OFS (0.75 % in the nursery and 0.5% in the grower-finisher) (T2), NC + high ZnO + OFS (T3), Positive control (PC), formulated to meet NRC (2012) nutrient requirements + high ZnO + AGP, (T4). All data were analyzed using PROC MIXED procedure of SAS (version 9.4; SAS Inst., Inc., Cary, NC). The model included fixed effect of treatment and random effect of replicate. Pen with 8 pigs was the experimental unit. Adjustment for multiple comparisons was by Tukey Kramer’s method. Overall (d 0 to 149), pigs on T4 diets had greater (0.47 vs 0.50; P = 0.013) and tended to have greater (0.48 vs 0.50; P = 0.083) G:F than pigs on the control (T1) and T2 diets, respectively. Relative to pigs on the T1 diet, ADG (0.796 vs 0.799 kg) and G:F (0.47 vs 0.48) of pigs fed the T2 diet were similar (P ˃0.10). Furthermore, pigs fed T4 and T3 diets did not differ in terms of ADFI (2.15 vs 2.14 kg; P = 0.994), ADG (0.804 vs 0.826 kg; P = 0.235), and G:F (0.48 vs 0.50; P = 0.260). Dietary treatments did not affect final BW, hot carcass weight, carcass yield, BF thickness, rib eye area, and percent lean (P ˃0.10). Based on performance criteria, these findings suggest OFS seaweed blend can replace either antibiotic growth promotors or pharmacological levels of zinc oxide in swine feeds.
PSIX-4 Concentrations of digestible and metabolizable energy and digestibility of phosphorus in a new source of high-protein distillers dried grains with solubles fed to growing pigs
Authors: Cristobal Romero minoy A , Lee Su A , Stein Hans H
Published: November 2020

Concentrations of digestible and metabolizable energy and digestibility of phosphorus in a new source of high-protein distillers dried grains with solubles fed to growing pigs Minoy Cristobal, Su A Lee, and Hans H. Stein The objective of this research was to test the hypothesis that concentrations of DE and ME and standardized total tract digestibility (STTD) of P in high-protein distillers dried grains with solubles (HP-DDGS) are greater than in conventional distillers dried grains with solubles (DDGS-CV). In Exp. 1, twenty-four growing barrows (initial BW: 32.7 ± 3.1 kg) were fed a corn diet or 2 diets containing corn and HP-DDGS or corn and DDGS-CV. The statistical model included ingredient as a fixed effects and replicate as the random effect and the pig was the experimental unit. Results from Exp. 1 indicated that the concentrations of DE and ME of HP-DDGS were greater (P < 0.05) compared with DDGS-CV (Table 1). In Exp. 2, thirty-two growing barrows (initial BW: 20.2 ± 0.9 kg) were allotted to 4 diets with 8 pigs per diet. The DDGS-CV or HP-DDGS were included in a diet without microbial phytase and in a diet with microbial phytase (500 units/kg diet). The statistical model included ingredient, phytase, and the interaction as fixed effects and replicate as the random effect. Results from Exp. 2 indicated that inclusion of phytase in the diet containing HP-DDGS increased (P < 0.05) the STTD of P, but addition of phytase to the DDGS-CV diet did not increase STTD of P (interaction, P < 0.001; Table 2). The value for the STTD of P was greater (P < 0.05) in DDGS-CV compared with HP-DDGS. In conclusion, HP-DDGS has greater concentrations of DE and ME, but less STTD of P compared with DDGS-CV.
PSIX-15 Extrusion of corn-soybean product blends in replacement of soybean meal in weanling pig diets
Authors: Milani Natália C , Paula Vinicius R C , Azevedo Cândida P F , Sedano Anderson A , Junior Hélio Moreira , Fernandes Raquel S , Andrade José M M , Ruiz Urbano S
Published: November 2020

Extrusion is a thermomechanical process that can reduce the content antinutritional factors in soybean, improving the use of its nutrients by pigs. The purpose of this study was to evaluate extruded corn-soybean grain blends, replacing soybean meal in weanling pig diets, on pig growth performance from 21 to 63 d old. Ground corn and the corn-soybean blend (75.27% of ground corn and 24.73%) were extruded at 120°C in a single-screw extruder. One hundred and sixty crossbred weaned pigs (6.02 ± 0.63 kg) were used in a three-phase feeding program (21-35, 36-49, 50-63 days of age) and fed a basal diet (BD - extruded corn, dairy product, spray dried plasma, and soybean meal) or one of four diets in which soybean meal was replaced for deactivated (DS) or extruded (EX) corn-soybean blend, in 50 or 100%, generating diets DS50, DS100, EX50 and EX100. Diets were formulated to provide 3.40, 3.38, and 3.20 Mcal of ME/kg; 220, 215, and 190 g/kg of CP; and 14.5, 13.3, and 10.9 g/kg of digestible lysine, in phases 1, 2, and 3, respectively. A randomized block design based on initial BW was used. The data was submitted to ANOVA and means were separated using Tukey test (5%). Pigs fed diets EX50 and EX100 presented reductions (P< 0.05) in ADG (16-50%), final BW and G:F (13-33%) compared to pigs submitted to diets SBM100, DS50 or DS100 in phases 1 and 2. ADG, ADFI, and G:F of pigs were not affected (P >0.05) in phase 3, but final BW of pigs fed extruded corn-soybean blend were lower (P< 0.05) than that of pigs fed other diets. The extrusion temperature may have been insufficient to inactivate the anti-nutritional factors of soybean that probably reduced pig growth performance. The inclusion of extruded corn-soybean blend in replacement of soybean meal reduced growth performance of pigs.
PSVI-9 Mint oil, yeast cell, and ɣ-tocopherol supplementation in gestation and lactation diets on offspring performance during the post-wean period
Authors: Hernandez Lily P , Dunn James L , Wenninghoff Joel , Levesque Crystal L
Published: November 2020

Weaning is a period of pig growth associated with increased incidence of oxidative stress which can impact post-weaning performance. Inclusion of feed additives with antioxidant activity in sow diets has been reported to improve offspring growth beyond the suckling phase. A study assessed yeast cell, mint oil, and ɣ-tocopherol supplementation in sow gestation and lactation diets has on offspring post-weaning performance. A total of 605 piglets (6.14 ± 2.53 kg BW) from 53 sows were randomly allotted to 62 pens, balanced by weight and litter within maternal diet [Control diet (CON), control + yeast cell at 0.15% (YC), control + mint oil at 10 ppm (MO), and control + ɣ-tocopherol at 200 ppm (GT)]. Pens of pigs were given a common diet for 126 d post-wean in a 9-phase feeding regimen. After d29 post-wean, performance of pigs deemed light (< 5.10 kg) and heavy (>7.25 kg) at weaning were followed to d126. Performance was analyzed as randomized complete block with pen as experimental unit and Tukey’s adjustment as means separation test. Pigs from CON sows tended to be lighter pigs from all other treatment groups at weaning and d29 post-wean due to differences in daily gain. Lightweight MO and GT pigs had a heavier weight at d42 (P=0.002) than CON and YC pigs (16.8, 16.7, 19.3, and 19.9 ± 0.77 kg in CON, YC, MO, and GT, respectively). At d70 post-wean, GT pigs tended to be heavier than CON pigs, with YC and MO intermediate. Lightweight pigs from MO sows had greater gain (P=0.04) during the finishing period than all other treatment groups, with GT pigs gaining less. There were no detectable differences in BW during the finishing phase among treatments in heavyweight pigs, however, CON pigs tended (P=0.07) to gain the least. Exposure to mint oil and ɣ-tocopherol during the prenatal and suckling period may provide lasting benefits to light-weight pigs post-wean.
PSVI-5 Effect of apple and anise flavour supplementation on growth performance, nutrient digestibility, blood profiles and carcass quality in growing-finishing pigs
Authors: Seok Woo Jung , Kim Yong Min , Ahn Je min , Liu Xiao , Ao Xiang , Kim In Ho
Published: November 2020

The present study was undertaken to determine the effects of different flavours (apple and anise) on growth performance, nutrient digestibility, blood profiles and carcass quality in growing-finishing pigs. A total of 96 pigs [(Landrace × Yorkshire) × Duroc] with an average initial BW of 28.2 ± 0.7 kg were assigned to 1 of 3 dietary treatments. The treatments consisted of (1) CON, basal diet; (2) APF, basal diet + 0.05% apple flavour; (3) ANF, basal diet + 0.05% anise flavour. There were 8 replications (pens) per treatment and 4 pigs per pen (2 barrows and 2 gilts). The flavour was added at the expense of corn (0.05%). The experiment lasted for 15 wks. The data were analyzed by ANOVA using the GLM procedure of SAS (SAS Institute Inc., Cary, NC, USA) with the pen as the experimental unit. Variability in the data was expressed as the standard error of means (SEM) and a probability level of p < 0.05 was considered to be statistically significant. During week 0-5, pigs fed ANF diets had greater (p < 0.05) ADG and ADFI than those fed CON and APF diets. Dietary ANF treatment increased (p < 0.05) ADG during 0-15 week compared with CON treatment (Table 1). At the end of 5 week, the apparent total tract digestibility of nitrogen in ANF treatment was improved (p < 0.05) compared with that in CON treatment (Table 1). There were no differences (p > 0.05) in the red blood cell and white blood cell counts or lymphocyte percentage among treatments. In conclusion, anise flavour supplementation could lead to significant improvement in ADG and N digestibility in growing pigs and the ADG was also higher in finishing pigs fed anise flavour supplemented diet. Furthermore, apple flavour supplementation had minor effect on growth performance, nutrient digestibility, blood profiles or carcass quality in finishing pigs.
PSVI-7 Increased consumption of Methionine as DL-Methionine or OH-Methionine improved pigs growing performance and carcass traits under hot conditions
Authors: da Silva Caio A , Callegari Marco A , Barbi José Henrique , Fagundes Naiara , Dias Cleandro P , Souza Kelly L , Batonon-Alavo Dolores I
Published: November 2020

This study aimed to determine the effects of increasing Methionine (Met) supply on growth performance and carcass traits of growing-finishing pigs under summer conditions in Brazil. A total of 200 barrows and females, 63 d-old (20.43±2.0 kg), were allocated in a 2 x 2 factorial design (two Met sources [DL-Met and OH-Met] and two Met doses [100 and 120% Brazilian Nutritional recommendation) with 10 replicates of 5 animals each. Corn-DDGS-wheat-soya based diets were formulated. Treatments were offered during four phases: growing I (63-93 d old), growing II (94-114 d old), finishing I (115-142 d old) and finishing II (143-160 d old). The recommended digestible Met levels were: 0.32, 0.30, 0.29 and 0.24% respectively for the four phases. Average temperature was 27.12 ± 4.51ºC and relative humidity was 61.72 ± 5.65%. Growth performance was measured for each rearing phase. Carcass yield, back fat height, loin depth, and lean meat yield were measured at the end of the experiment. Data were submitted to a 2-way ANOVA and Tukey test. There was no significant interaction between the Met source and the dose for all performance and carcass traits. Feed intake and feed conversion ratio were similar between treatments (P >0.05). The individual daily weight gain and live weight were better for the highest doses of methionine (P< 0.05) in all phases and on the whole period, except for the finishing II where the weight gain was similar for all treatments. Likewise, carcass yield, lean meat was increased with the highest dose of methionine in comparison to the adequate dose. No significant difference was found between DL-Met and OH-Met on growth performance parameters. OH-Met resulted in a higher loin depth (58.37 mm) in comparison to DL-Met (55.21 mm). Overall, these results demonstrated the interest of increasing Met supply under hot conditions to improving performance and carcass quality traits.
PSI-5 Effects of rest period prior to processing on anthelmintic response during the receiving period in feedlot heifers
Authors: Stafford Nicole , Tarpoff A J , Theurer Miles , Jones Tom , Jones Cassandra K
Published: November 2020

A major stressor affecting feedlot cattle performance is transportation. Knowing when to process cattle and how much rest to provide prior to processing may improve cattle health and response to processing, such as anthelmintic administration. The goal of this project was to determine the impact of time of rest prior to initial processing on parasite prevalence during the receiving period. Eighty mixed-breed heifers (250±4.2 kg BW) were purchased at live auction in Oklahoma City, OK and transported to the Kansas State University Beef Cattle Research Center in Manhattan. Heifers were allotted in a completely randomized design to one of four treatments processed at 0, 6, 24, or 48 hours after arrival. At processing (d 0), fecal samples were collected, and cattle were subcutaneously injected with 1.0 ml/50 kg BW moxidectin (Cydectin®, Bayer Animal Health, Shawnee Mission, KS) and orally dosed with 1.0 mL/50 kg BW oxfendazole (Synanthic®, Boehringer Ingelheim, St. Joseph, MO). Fecal samples were collected again on d 14. Fecal samples were analyzed by the Kansas State University Veterinary Diagnostic Laboratory for qualitative and quantitative fecal float. Time of processing did not impact (P > 0.05) any measured response criteria. On d 0, there was a high prevalence of fecal parasites, which was significantly reduced by d 14 (94.5% vs. 23.1% of cattle with detected fecal parasites on d 0 vs. 14, respectively). On d 0, semi-quantitative density showed the highest concentration of strongyle and eimeria parasites, which were significantly reduced (P < 0.05) by d 14 (315 and 155 vs. 2 and 6.5 eggs/g of feces detected for strongyle vs. eimeria, respectively). In summary, time of rest prior to processing had no detected impact on anthelmintic response, but the dual injection/oral protocol used in this experiment was highly effective at reducing parasite levels within 2 weeks of administration.
PSI-2 Effects of extended release eprinomectin on heifer performance while grazed on endophyte-infected tall fescue or housed in drylot
Authors: Navarrete Julissa , Graham Sarah , Neira Lucas , McCann Joshua C , Shike Daniel W
Published: November 2020

Objectives were to evaluate the effects of LongRange, an extended-release eprinomectin, on beef heifer performance while grazed on endophyte-infected tall fescue or housed in drylot. Fall-born, Angus × Simmental heifers (n = 153) were utilized in a split-plot design. Heifers were stratified by BW and assigned to 6 groups. Groups were randomly assigned to 1 of 2 environmental treatments: grazed endophyte-infected tall fescue (Pasture) or housed in drylot and fed alfalfa hay. Within environmental treatment groups, heifers were randomly assigned to LongRange (LR) or control (Saline). Prior to experiment initiation, heifers were treated with oral fenbendazole (SafeGuard) to minimize parasite load. Data were analyzed using the MIXED procedure of SAS. At d 0, 56, and 112, heifer BW, ADG, BCS, hair coat score (HCS), and fecal egg counts (FEC) were collected. Treatments did not affect (P ≥ 0.17) BW on d 0 and 56. On d 112, drylot heifers tended (P = 0.08) to be heavier than pasture heifers and LR increased (P = 0.01) BW. There was an interaction (P = 0.02) for ADG; LR increased ADG to a greater magnitude in pasture than drylot heifers. On d 0, 56, and 112, there was no interaction or LongRange effect (P ≥ 0.11) on BCS; however, pasture heifers had greater (P = 0.05) d 112 BCS than drylot heifers. On d 56, there was an interaction (P = 0.01) on HCS; LR decreased HCS in pasture heifers, but did not affect HCS in drylot heifers. Although treatment did not effect (P ≤ 0.33) FEC at d 0, there was an interaction (P = 0.01) observed on d 56. LongRange decreased FEC in pasture heifers but not in drylot. In conclusion, LongRange increased heifer growth performance with a greater difference for heifers grazing tall fescue, compared with heifers in drylot.
PSI-9 Factors associated with birthing interval and total farrowing duration in sows and gilts within a production setting
Authors: Kovarna Madison , Perez-Palencia Jorge Y , Levesque Crystal L
Published: November 2020

Common sow barn protocol is to provide farrowing assistance (i.e. sleeving) when 20 min has elapsed since the previous piglet to reduce the incidence of stillbirths with limited consideration for parity, litter size or sow energy status. The objective of this study was to evaluate the impact of litter size, birth order, parity, and feed intake before farrowing on total farrowing duration and birthing interval. Farrowing duration and piglet birth interval were measured in 80 farrowings (PIC 1050 gilts and multiparous). Farrowing was supervised by trained technicians 24 h/d starting at d114 of gestation. The time of each piglet birth was documented, and the total farrowing duration was determined as the time elapsed between the birth of the first and last piglet. Sleeving protocol used when 60 min had elapsed since the previous piglet, oxytocin provided when farrowing was deemed complete to aid in milk let down. Information from the electronic lactation feeding system was used to characterize the feed intake pattern of sows before farrowing. Data was analyzed by PROC MIXED procedure (SAS Inst., Inc., Cary, NC). The overall rate of stillbirths was 8.3%. Sows with litters >14 piglets had shorter birthing interval than sows with litters < 14 piglets (P < .0001) resulting in 9 min shorter total farrowing with larger litter sizes (Table 1). Order of birth affected birth interval (P = 0.014), where piglets of order 1 to 4 had the longest birth interval and orders 9 to 12 shortest. Sow feed intake prior to farrowing and parity did not affect total farrowing duration or birthing interval; however, farrowing duration of mid parity sows was 30 to 50 min shorter than young and older females. Sow barn farrowing care protocols may need to be revised considering sow parity and number of piglets born.
PSIII-6 Effects of repeated freeze and thaw cycles on serum and plasma metabolite concentrations in beef cattle
Authors: Monaghan Jera L , Rathert Abigail R , Meyer Allison M
Published: November 2020

The objective of this study was to determine whether repeated freeze and thaw cycles alter bovine circulating metabolite concentrations, which serve as indicators of nutrient utilization. In research settings, it is common to thaw and refreeze samples when confirming results or performing further analyses; thus, it is important to determine if these actions alter metabolites. Jugular blood samples were collected from beef cows (n = 14) and fall-born calves (n = 14) at weaning. Samples were processed into multiple aliquots and stored at -20°C until analysis. Samples were thawed at 4°C for 8 to 10 h on each day of analysis and refrozen for at least 48 h between cycles. Concentrations of serum non-esterified fatty acids (NEFA), serum urea N, plasma triglycerides, and plasma glucose were analyzed using commercially available kits on a UV-visible light plate reader. Data were analyzed with sample type (cow vs. calf), thaw (cycles 1-4), and their interaction in a mixed model. Additionally, R2 were determined among freeze-thaw cycles within each metabolite. Thaw cycle impacted glucose concentrations (P = 0.03); other metabolites were unaffected (P > 0.25). Thaw cycle and its interaction with sample type did not affect metabolite concentrations (P > 0.33). Sample type did not affect triglyceride and BUN concentrations (P > 0.34). There was a type effect (P < 0.0001) for NEFA and glucose; NEFA were greater in cows and glucose was greater in calves. There was a strong relationship between urea N thaw cycles 1 and 2 (R2 = 0.88), which was also apparent between all other urea N, triglycerides, NEFA, and glucose cycles (R2 ≥ 0.79, 0.96, 0.91, and 0.61 respectively). These data indicate that repetitive freezing and thawing of bovine serum and plasma samples has little effect on non-esterified fatty acids, triglycerides, and urea N, but may impact glucose concentrations.
PSIII-7 Evaluating sow colostrum somatic cell count as an indicator for colostrum composition or litter characteristics
Authors: Maurer Ryan T , Gourley Kiah M , Rathbun Theresa J , Woodworth Jason C , DeRouchey Joel M , Tokach Mike D , Dritz Steve S , Goodband Robert D
Published: November 2020

Sow colostrum is essential during early piglet life to provide passive immunity via immunoglobulins and leukocytes.The somatic cell population in colostrum and milk consists of leukocytes and epithelial cells. Somatic cell count (SCC) of milk is commonly used as an indicator of cow milk quality and health status, but not commonly measured in lactating gilts or sows. An experiment was conducted to evaluate the relationship between colostrum SCC and colostrum composition or litter performance. A total of 194 frozen (-20℃) colostrum samples from Large White × Landrace females were evaluated for SCC. Cells were pelleted and washed twice by centrifugation (10 min. at 400 x g; 4℃) in Phosphate Buffered Saline (PBS; pH 7.2, LifeTechnologies). The resulting cell sample was labelled with a nuclear dye (LDS751) and cells counted via micro-capillary bench-top flow cytometer (Guava EasyCyte Plus, Millipore). Final SCC (cells/mL) was calculated by dividing sample cell count (cells/sample) by original sample volume (mL/sample). Data was analyzed for relationship between SCC and response variables using Pearson correlation. A pairwise comparison was used to evaluate SCC by parity category (gilts vs. sows). Results were considered significant at P< 0.05. Final somatic cell counts ranged from 5.8×104 to 2.9×106 cells/mL. Sows had decreased (P=0.033) SCC compared to gilts (3.0 ×105 vs. 3.7 ×105). There was no evidence for a relationship (P >0.05) between SCC and piglet weight at 24h or weaning, 24h litter gain, 24h or pre-wean mortality, sow backfat or body weight. Colostrum total solids, protein, lactose, and immunoglobulin G concentration showed no evidence for relationship (P >0.05) to SCC. Colostrum fat showed a weak positive correlation (P=0.018, R=0.18) with SCC. In conclusion, colostrum SCC is lower in sows than gilts, but does not appear to correlate to colostrum composition or litter performance.
4 Examining x, y, and z vibration patterns of commercial pig transport trailers from the farm to the abattoir
Authors: Gonzalez John M , Davis Ben , Morris Benjamin , Brokesh Edwin , Flippo Daniel , Houser Terry , Najar-Villarreal Francisco , Turner Kari , Williams Jamison
Published: November 2020

The objective of this study was to measure and examine 3-axis acceleration data from 6 locations within commercial transport trailers shipping market pigs. Over winter months (December through February) of 2018 to 2019 and 2019 to 2020, 16 pot-belly and 14 straight-deck trailer loads of market pigs were measured from 2 producers located in Kansas and North Carolina, respectively. Six accelerometers were placed in protective cases and affixed to the underside of the floor in the approximate center of the front compartment, middle of the trailer, and back compartment of the top and bottom decks. Data were post-processed to calculate power spectral density (PSD) functions and corresponding root mean square (RMS) accelerations. The PSDs lend insight into the vibrational frequency content of the trailers, while the RMS values indicate the severity of the vibration over the duration of each trip. With the exception of the lower aft portion of the trailer where levels are significantly higher, RMS values were consistent across trips and largely similar between sensor location and axis. Accelerations ranged between 0.06 and 0.18 g and varied in time, indicating data were non-stationarity. The PSD results reveal a largely broadband frequency response of the loaded trailers between 0 and 50 Hz, especially for sensors on the lower deck. Preliminary analysis of the data indicates the severity of vibrations experienced by pigs during transport would be considered uncomfortable by humans.
5 Effect of rest stop on welfare indicators of conditioned and non-conditioned ranch-direct or auction-market-sourced beef cattle, transported by road
Authors: Meléndez Daniela M , Marti Sonia , Schwinghamer Timothy , Haley Derek B , Nordi Wiolene M , Schwartzkopf-Genswein Karen S
Published: November 2020

The aim of this study was to assess the effect of an 8h rest stop after 36 h of road transport on physiological and behavioral indicators of welfare in 7–8-mo-old conditioned and non-conditioned beef calves, sourced from either a ranch or an auction market. Three hundred and twenty weaned calves (245 ± 35.7 kg BW) were randomly assigned to a 2 × 2 × 2 factorial design: conditioning, conditioned (C) or non-conditioned (N); source, ranch direct (R) or auction market (A); and rest, 0 (R0) or 8 (R8) h. After resting, animals were transported for an additional 4 h. A subset of 12 animals/treatment were sampled for non-esterified fatty acids (NEFA), serum amyloid-A (SAA) and creatine kinase (CK) concentrations prior to the first loading (L1); after 36 h of transport; prior to and after the additional 4 h of transport, on the day of arrival (0); and 1, 2, 3, 5, 14, and 28 d after the 4h transport. Daily standing % and dry matter intake (DMI) were assessed for 3 d after transport. Data were analyzed using the GLIMMIX procedure of SAS. Fixed effects included conditioning, source, and time (nested in rest), while random effects were animal and pen. At L1, mean concentrations of NEFA, SAA and CK were greater (P < 0.05) for N-R0 than C-R0 and for N-R8 than C-R8. The N and R groups had greater (P < 0.05) standing % than the C and A groups, respectively. On d 0 and 1, the C-R0 and C-R8 groups had greater (P < 0.05) DMI than the N-R0 and N-R8 groups, respectively. Overall, physiological and behavioral indicators of welfare were greater in conditioned than non-conditioned calves, while fewer differences were observed between ranch and auction market calves, as well as rested or unrested calves.
2 Evaluating the effects of a dietary strategy to mitigate the impact of in utero heat stress on biomarkers of post-absorptive metabolism and stress in pigs following weaning and transport
Authors: Maskal Jacob M , Duttlinger Alan W , Kpodo Kouassi R , McConn Betty R , Byrd Christopher , Richert Brian T , Lay Donald C , Marchant-Forde Jeremy , Lucy Matthew , Safranski Tim , Perry Shelbi , Johnson Jay S
Published: November 2020

In utero heat stress (IUHS) increases postnatal energy requirements of pigs, and this may exacerbate weaning and transport stress. The study objective was to evaluate the impact of providing a nutrient dense (ND) diet, which met the greater energy requirements of IUHS pigs, on biomarkers of post-absorptive metabolism and stress following weaning and transport. Twenty-four pregnant gilts were assigned to thermoneutral (n = 12; 17.5 ± 2.1°C) or heat stress (n = 12; cyclical 26°C to 36°C) conditions from gestation d 1 to 58, and then TN conditions until farrowing. At weaning (16.2 ± 0.4 d), mixed-sex piglets (n = 160; 4.78 ± 0.15 kg BW) were transported (loading + transport + unloading) for 11 h 40 min and then blocked into pens (n = 4 piglets/pen) by in utero and dietary treatments fed for 14 d (P1): in utero thermoneutral (IUTN) + control diet (C; n = 10 pens), IUTN + ND (n = 10 pens), IUHS + C (n = 10 pens), IUHS + ND (n = 10 pens). The C diet was fed to all pigs from d 15 to 35 post-weaning and transport (P2). One pig per pen was bled immediately pre-transport (Pre-T) and immediately post-transport (Post-T), and on d 2, 7, 14, 28, and 35 post-weaning and transport to analyze cortisol, glucose, insulin, and non-esterified fatty acids (NEFA). Overall, cortisol was decreased (P = 0.03; 25.8%) during transport in IUHS versus IUTN pigs. On d 2, glucose was decreased overall (P = 0.01; 13.5 mg/dL) in IUHS versus IUTN pigs, but no d 2 insulin (P = 0.76; 0.079 ± 0.011 ng/mL) or NEFA (P = 0.65; 810.3 ± 61.1 mEq/L) differences were detected. Insulin and insulin:glucose ratio were increased (P < 0.04; 0.076 ng/mL and 0.05, respectively) during P1 in ND versus C pigs. In summary, the physiological stress response and glucose levels were altered in IUHS versus IUTN pigs post-weaning and transport, but dietary treatment did not interact with in utero treatment.