bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and Optimization of Lipase-Catalyzed Synthesis of Phospholipids Containing 3,4-Dimethoxycinnamic Acid by Response Surface Methodology
Authors: Rychlicka Magdalena , Niezgoda Natalia , Gliszczyńska Anna
Published: May 2020

The interesterification reaction of egg-yolk phosphatidylcholine (PC) with ethyl ester of 3,4-dimethoxycinnamic acid (E3,4DMCA) catalyzed by Novozym 435 in hexane as a reaction medium was shown to be an effective method for the synthesis of corresponding structured O-methylated phenophospholipids. The effects of substrate molar ratios, time of the reaction and enzyme load on the process of incorporation of 3,4DMCA into PC were evaluated by using the experimental factorial design of three factors and three levels. The results showed that a substrate molar ratio is a crucial variable for the maximization of the synthesis of 3,4-dimethoxycinnamoylated phospholipids. Under optimized parameters of 1/10 substrate molar ratio PC/E3,4DMCA, enzyme load 30% (w/w), hexane as a medium and incubation time of 3 days, the incorporation of aromatic acid into phospholipid fraction reached 21 mol%. The modified phosphatidylcholine (3,4DMCA-PC) and modified lysophosphatidylcholine (3,4DMCA-LPC) were obtained in isolated yields of 3.5% and 27.5% (w/w), respectively. The developed method of phosphatidylcholine interesterification is the first described in the literature dealing with 3,4DMCA and allows us to obtain new O-methylated phenophospholipids with potential applications as food additives or nutraceuticals with pro-health activity.
CuII- and CoII-Based MOFs: {[La2Cu3(µ-H2O)(ODA)6(H2O)3]∙3H2O}n and {[La2Co3(ODA)6(H2O)6]∙12H2O}n. The Relevance of Physicochemical Properties on the Catalytic Aerobic Oxidation of Cyclohexene
Authors: Santibáñez Luis , Escalona Néstor , Torres Julia , Kremer Carlos , Cancino Patricio , Spodine Evgenia
Published: May 2020

The aerobic oxidation of cyclohexene was done using the heterometallic metal organic frameworks (MOFs) {[La2Cu3(μ-H2O)(ODA)6(H2O)3]⋅3H2O}n (LaCuODA)) (1) and {[La2Co3(ODA)6(H2O)6]∙12H2O}n (LaCoODA) (2) as catalysts, in solvent free conditions (ODA, oxydiacetic acid). After 24 h of reaction, the catalytic system showed that LaCoODA had a better catalytic performance than that of LaCuODA (conversion 85% and 67%). The structures of both catalysts were very similar, showing channels running along the c axis. The physicochemical properties of both MOFs were determined to understand the catalytic performance. The Langmuir surface area of LaCoODA was shown to be greater than that of LaCuODA, while the acid strength and acid sites were greater for LaCuODA. On the other hand, the redox potential of the active sites was related to CoII/CoIII in LaCoODA and CuII/CuI in LaCuODA. Therefore, it is concluded that the Langmuir surface area and the redox potentials were more important than the acid strength and acid sites of the studied MOFs, in terms of the referred catalytic performance. Finally, the reaction conditions were also shown to play an important role in the catalytic performance of the studied systems. Especially, the type of oxidant and the way to supply it to the reaction medium influenced the catalytic results.
ADMET Polymerization of Dimeric Cinchona Squaramides for the Preparation of a Highly Enantioselective Polymeric Organocatalyst
Authors: Ullah Mohammad Shahid , Chhanda Sadia Afrin , Itsuno Shinichi
Published: May 2020

Under the acyclic diene metathesis (ADMET) reaction condition, the C3-vinyl groups of cinchona alkaloids readily react with each other to form a C-C bond. A novel type of cinchona alkaloid polymers was synthesized from dimeric cinchona squaramides using the Hoveyda-Grubbs’ second-generation catalysts (HG2) by means of ADMET reaction. The chiral polymers, containing cinchona squaramide moieties in their main chains, were subsequently employed as catalysts for the enantioselective Michael reaction to give the corresponding chiral adducts in high yields with excellent enantioselectivity and diastereoselectivity. Both enantiomers from the asymmetric Michael reaction were distinctively prepared while using the polymeric catalysts, possessing pseudoenantiomeric structures. The catalysts were readily recovered from the reaction mixture and recycled several times due to the insolubility of the cinchona-based squaramide polymers.
The Role of Tumor-Associated Macrophages in the Progression and Chemoresistance of Ovarian Cancer
Authors: Nowak Marek , Klink Magdalena
Published: May 2020

Tumor-associated macrophages (TAMs) constitute the main population of immune cells present in the ovarian tumor microenvironment. These cells are characterized by high plasticity and can be easily polarized by colony-stimulating factor-1, which is released by tumor cells, into an immunosuppressive M2-like phenotype. These cells are strongly implicated in both the progression and chemoresistance of ovarian cancer. The main pro-tumoral function of M2-like TAMs is the secretion of a variety of cytokines, chemokines, enzymes and exosomes that reach microRNAs, directly inducing the invasion potential and chemoresistance of ovarian cancer cells by triggering their pro-survival signaling pathways. The M2-like TAMs are also important players in the metastasis of ovarian cancer cells in the peritoneum through their assistance in spheroid formation and attachment of cancer cells to the metastatic area—the omentum. Moreover, TAMs interplay with other immune cells, such as lymphocytes, natural killer cells, and dendritic cells, to inhibit their responsiveness, resulting in the development of immunosuppression. The detrimental character of the M2-like type of TAMs in ovarian tumors has been confirmed by a number of studies, demonstrating the positive correlation between their high level in tumors and low overall survival of patients.
Epigenetic Features of Human Perinatal Stem Cells Redefine Their Stemness Potential
Authors: Gaggi Giulia , Di Credico Andrea , Izzicupo Pascal , Antonucci Ivana , Crescioli Clara , Di Giacomo Viviana , Di Ruscio Annalisa , Amabile Giovanni , Alviano Francesco , Di Baldassarre Angela , Ghinassi Barbara
Published: May 2020

Human perinatal stem cells (SCs) can be isolated from fetal annexes without ethical or safety limitations. They are generally considered multipotent; nevertheless, their biological characteristics are still not fully understood. The aim of this study was to investigate the pluripotency potential of human perinatal SCs as compared to human induced pluripotent stem cells (hiPSCs). Despite the low expression of the pluripotent factors NANOG, OCT4, SOX2, and C-KIT in perinatal SC, we observed minor differences in the promoters DNA-methylation profile of these genes with respect to hiPSCs; we also demonstrated that in perinatal SCs miR-145-5p had an inverse trend in comparison to these stemness markers, suggesting that NANOG, OCT4, and SOX2 were regulated at the post-transcriptional level. The reduced expression of stemness markers was also associated with shorter telomere lengths and shift of the oxidative metabolism between hiPSCs and fetal annex-derived cells. Our findings indicate the differentiation ability of perinatal SCs might not be restricted to the mesenchymal lineage due to an epigenetic barrier, but other regulatory mechanisms such as telomere shortening or metabolic changes might impair their differentiation potential and challenge their clinical application.
γδ T Cells: The Ideal Tool for Cancer Immunotherapy
Authors: Yazdanifar Mahboubeh , Barbarito Giulia , Bertaina Alice , Airoldi Irma
Published: May 2020

γδ T cells have recently gained considerable attention as an attractive tool for cancer adoptive immunotherapy due to their potent anti-tumor activity and unique role in immunosurveillance. The remarkable success of engineered T cells for the treatment of hematological malignancies has revolutionized the field of adoptive cell immunotherapy. Accordingly, major efforts are underway to translate this exciting technology to the treatment of solid tumors and the development of allogeneic therapies. The unique features of γδ T cells, including their major histocompatibility complex (MHC)-independent anti-cancer activity, tissue tropism, and multivalent response against a broad spectrum of the tumors, render them ideal for designing universal ‘third-party’ cell products, with the potential to overcome the challenges of allogeneic cell therapy. In this review, we describe the crucial role of γδ T cells in anti-tumor immunosurveillance and we summarize the different approaches used for the ex vivo and in vivo expansion of γδ T cells suitable for the development of novel strategies for cancer therapy. We further discuss the different transduction strategies aiming at redirecting or improving the function of γδ T cells, as well as, the considerations for the clinical applications.
Joint Reconstituted Signaling of the IL-6 Receptor via Extracellular Vesicles
Authors: Arnold Philipp , Lückstädt Wiebke , Li Wenjia , Boll Inga , Lokau Juliane , Garbers Christoph , Lucius Ralph , Rose-John Stefan , Becker-Pauly Christoph
Published: May 2020

Interleukin-6 (IL-6) signaling is a crucial regulatory event important for many biological functions, such as inflammation and tissue regeneration. Accordingly, several pathological conditions are associated with dysregulated IL-6 activity, making it an attractive therapeutic target. For instance, blockade of IL-6 or its α-receptor (IL-6R) by monoclonal antibodies has been successfully used to treat rheumatoid arthritis. However, based on different signaling modes, IL-6 function varies between pro- and anti-inflammatory activity, which is critical for therapeutic intervention. So far, three modes of IL-6 signaling have been described, the classic anti-inflammatory signaling, as well as pro-inflammatory trans-signaling, and trans-presentation. The IL-6/IL-6R complex requires an additional β-receptor (gp130), which is expressed on almost all cells of the human body, to induce STAT3 (signal transducer and activator of signal transcription 3) phosphorylation and subsequent transcriptional regulation. In contrast, the IL-6R is expressed on a limited number of cells, including hepatocytes and immune cells. However, the proteolytic release of the IL-6R enables trans-signaling on cells expressing gp130 only. Here, we demonstrate a fourth possibility of IL-6 signaling that we termed joint reconstituted signaling (JRS). We show that IL-6R on extracellular vesicles (EVs) can also be transported to and fused with other cells that lack the IL-6R on their surface. Importantly, JRS via EVs induces delayed STAT3 phosphorylation compared to the well-established trans-signaling mode. EVs isolated from human serum were already shown to carry the IL-6R, and thus this new signaling mode should be considered with regard to signal intervention.
Molecular Chaperones and Proteolytic Machineries Regulate Protein Homeostasis in Aging Cells
Authors: Margulis Boris , Tsimokha Anna , Zubova Svetlana , Guzhova Irina
Published: May 2020

Throughout their life cycles, cells are subject to a variety of stresses that lead to a compromise between cell death and survival. Survival is partially provided by the cell proteostasis network, which consists of molecular chaperones, a ubiquitin-proteasome system of degradation and autophagy. The cooperation of these systems impacts the correct function of protein synthesis/modification/transport machinery starting from the adaption of nascent polypeptides to cellular overcrowding until the utilization of damaged or needless proteins. Eventually, aging cells, in parallel to the accumulation of flawed proteins, gradually lose their proteostasis mechanisms, and this loss leads to the degeneration of large cellular masses and to number of age-associated pathologies and ultimately death. In this review, we describe the function of proteostasis mechanisms with an emphasis on the possible associations between them.
Platelet Transforming Growth Factor-β1 Induces Liver Sinusoidal Endothelial Cells to Secrete Interleukin-6
Authors: Balaphas Alexandre , Meyer Jeremy , Perozzo Remo , Zeisser-Labouebe Magali , Berndt Sarah , Turzi Antoine , Fontana Pierre , Scapozza Leonardo , Gonelle-Gispert Carmen , Bühler Leo
Published: May 2020

The roles and interactions of platelets and liver sinusoidal endothelial cells in liver regeneration are unclear, and the trigger that initiates hepatocyte proliferation is unknown. We aimed to identify the key factors released by activated platelets that induce liver sinusoidal endothelial cells to produce interleukin-6 (IL-6), a cytokine implicated in the early phase of liver regeneration. We characterized the releasate of activated platelets inducing the in vitro production of IL-6 by mouse liver sinusoidal endothelial cells and observed that the stimulating factor was a thermolabile protein. Following gel filtration, a single fraction of activated platelet releasate induced a maximal IL-6 secretion by liver sinusoidal endothelial cells (90.2 ± 13.9 versus control with buffer, 9.0 ± 0.8 pg/mL, p < 0.05). Mass spectroscopy analysis of this fraction, followed by in silico processing, resulted in a reduced list of 18 candidates. Several proteins from the list were tested, and only recombinant transforming growth factor β1 (TGF-β1) resulted in an increased IL-6 production up to 242.7 ± 30.5 pg/mL, which was comparable to non-fractionated platelet releasate effect. Using neutralizing anti-TGF-β1 antibody or a TGF-β1 receptor inhibitor, IL-6 production by liver sinusoidal endothelial cells was dramatically reduced. These results support a role of platelet TGF-β1 β1 in the priming phase of liver regeneration.
Pipeline for the Generation and Characterization of Transgenic Human Pluripotent Stem Cells Using the CRISPR/Cas9 Technology
Authors: Mianné Joffrey , Bourguignon Chloé , Nguyen Van Chloé , Fieldès Mathieu , Nasri Amel , Assou Said , De Vos John
Published: May 2020

Recent advances in genome engineering based on the CRISPR/Cas9 technology have revolutionized our ability to manipulate genomic DNA. Its use in human pluripotent stem cells (hPSCs) has allowed a wide range of mutant cell lines to be obtained at an unprecedented rate. The combination of these two groundbreaking technologies has tremendous potential, from disease modeling to stem cell-based therapies. However, the generation, screening and molecular characterization of these cell lines remain a cumbersome and multi-step endeavor. Here, we propose a pipeline of strategies to efficiently generate, sub-clone, and characterize CRISPR/Cas9-edited hPSC lines in the function of the introduced mutation (indels, point mutations, insertion of large constructs, deletions).
Visfatin Increases VEGF-Dependent Angiogenesis of Endothelial Progenitor Cells during Osteoarthritis Progression
Authors: Tsai Chun-Hao , Liu Shan-Chi , Chung Wen-Hui , Wang Shih-Wei , Wu Min-Huan , Tang Chih-Hsin
Published: May 2020

Osteoarthritis (OA) pannus contains a network of neovascularization that is formed and maintained by angiogenesis, which is promoted by vascular endothelial growth factor (VEGF). Bone marrow-derived endothelial progenitor cells (EPCs) are involved in VEGF-induced vessel formation in OA. The adipokine visfatin stimulates the release of inflammatory cytokines during OA progression. In this study, we found significantly higher visfatin and VEGF serum concentrations in patients with OA compared with healthy controls. We describe how visfatin enhanced VEGF expression in human OA synovial fibroblasts (OASFs) and facilitated EPC migration and tube formation. Treatment of OASFs with PI3K and Akt inhibitors or siRNAs attenuated the effects of visfatin on VEGF synthesis and EPC angiogenesis. We also describe how miR-485-5p negatively regulated visfatin-induced promotion of VEGF expression and EPC angiogenesis. In our OA rat model, visfatin shRNA was capable of inhibiting visfatin and rescuing EPC angiogenesis and pathologic changes. We detail how visfatin affected VEGF expression and EPC angiogenesis in OASFs by inhibiting miR-485-5p synthesis through the PI3K and Akt signaling pathways.
In Vitro Modeling of Reoxygenation Effects on mRNA and Protein Levels in Hypoxic Tumor Cells upon Entry into the Bloodstream
Authors: Bartkowiak Kai , Koch Claudia , Gärtner Sebastian , Andreas Antje , Gorges Tobias M , Pantel Klaus
Published: May 2020

Background: Solid epithelial tumors like breast cancer are the most frequent malignancy in women. Circulating tumor cells (CTCs) are frequently released from hypoxic areas into the blood, where CTCs face elevated oxygen concentrations. This reoxygenation might challenge the use of CTCs for liquid biopsy. Methods: We modeled this situation in vitro using the breast cancer cell lines—MCF-7, MDA-MB-468, MDA-MB-231—and the cell line BC-M1 established from DTCs in the bone marrow. Cells were cultured under hypoxia, followed by a reoxygenation pulse for 4 h, reflecting the circulation time of CTCs. Analyzed were gene products like EGFR, ErbB-2, EpCAM, PD-L1 on mRNA and protein level. Results: mRNAs of erbb2 or pdl1 and protein levels of PD-L1 displayed significant changes, whereas ErbB-2 protein levels remained constant. The strongest discrepancy between protein and mRNA levels under hypoxia was observed for EGFR, supporting the idea of cap-independent translation of egfr mRNA. Analyses of the phosphorylation of AKT, Erk 1/2, and Stat3 revealed strong alterations after reoxygenation. Conclusions: CTCs reaching secondary sites faster than reoxygenation could alter the mRNA and protein levels in the cells. CTC and DTC with high PD-L1 levels might become quiescent under hypoxia but were easily reactivated by reoxygenation.
Investigation of Conductive Mechanism of Amorphous IGO Resistive Random-Access Memory with Different Top Electrode Metal
Authors: Huang Wei-Lun , Lin Yong-Zhe , Chang Sheng-Po , Chang Shoou-Jinn
Published: May 2020

In this paper, resistive random-access memory (RRAM) with InGaO (IGO) as an active layer was fabricated by radio-frequency (RF) sputtering system and the resistive switching mechanism with the different top electrode (TE) of Pt, Ti, and Al were investigated. The Pt/IGO/Pt/Ti RRAM exhibits typical bipolar resistive switching features with an average set voltage of 1.73 V, average reset voltage of −0.60 V, average high resistance state (HRS) of 54,954.09 Ω, and the average low resistance state (LRS) of 64.97 Ω, respectively. Ti and Al were substituted for Pt as TE, and the conductive mechanism was different from TE of Pt. When Ti and Al were deposited onto the switching layer, both TE of Ti and Al will form oxidation of TiOx and AlOx because of their high activity to oxygen. The oxidation will have different effects on the forming of filaments, which may further affect the RRAM performance. The details of different mechanisms caused by different TE will be discussed. In brief, IGO is an excellent candidate for the RRAM device and with the aids of TiOx, the set voltage, and reset voltage, HRS and LRS become much more stable.
Antioxidant Activities of Jeju Wax Apple (Syzygium samarangense) and Safety of Human Keratinocytes and Primary Skin Irritation Test
Authors: Hyun Su Bin , Bae Sungmin , Hyun Chang-Gu
Published: May 2020

This study analyzed the antioxidant activity, cell viability, and human skin primary irritation test using the hot-water extracts of the Syzygium samarangense. As a result of the recent warmer climate, tropical plants have flourished on Jeju Island, and S. samarangense is one of these plants known to have biological activities. In this study, the hot-water extract of S. samarangense leaf and branch was analyzed. Antioxidant activity was measured by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid)) assays, and the DMPD (dimethyl-4-phenylenediamine) radical scavenging activity, nitrite scavenging activity, ferrous-ion chelating activity, cupric reducing antioxidant capacity, reducing power assay, ferric reducing antioxidant power, total phenol content, and total flavonoid content were also measured. In addition, cell viability was measured by MTT assay in human keratinocyte cells (HaCaT), and the safety of the extract for use on the skin was evaluated in the human skin primary irritation test. The antioxidant activities, except DMPD radical scavenging activity and ferrous-ion chelating activity, were stronger in the branch extract than in leaf extract, and the total phenol and flavonoid contents were also higher in the branch extract. Slight irritation was observed in the human skin primary irritation test. However, it was possible to observe sufficient antioxidant capacity at a concentration lower than the concentration used in the irritation test; therefore, if the concentration of the extract is appropriately adjusted, this suggests that it is a possible natural material suitable for use in cosmetics.
Exploring SiC Planar IGBTs towards Enhanced Conductivity Modulation Comparable to SiC Trench IGBTs
Authors: Zhang Meng , Li Baikui , Wei Jin
Published: May 2020

The state-of-the-art silicon insulated-gate bipolar transistor (IGBT) features a trench gate, since it enhances the conductivity modulation. The SiC trench IGBT, however, faces the critical challenge of a high electric field in the gate oxide, which is a crucial threat to the device’s reliability. In this work, we explore the possibility of using a SiC planar IGBT structure to approach high performance to the level of a SiC trench IGBT, without suffering the high gate oxide field. The proposed SiC planar IGBT features buried p-layers directly under the p-bodies, and thus can be formed using the same mask set. The region between the buried p-layer and the p-body is heavily doped with n-type dopants so that the conductivity modulation is improved. Comprehensive TCAD simulations have been carried out to verify this concept, and the simulation results show the new SiC planar IGBT exhibits a high performance comparable to the trench IGBT, and also exhibits a low gate oxide field.
Differences in the Olfactory Sensitivity of Ceratitis capitata to Headspace of Some Host Plants in Relation to Sex, Mating Condition and Population
Authors: Sollai Giorgia , Solari Paolo , Crnjar Roberto
Published: May 2020

The Mediterranean fruit fly, Ceratitis capitata Wied., is among the most serious pests in horticulture worldwide, due to its high reproductive potential, difficulty of control and broad polyphagy. The aim of this study was to measure—by means of the electroantennogram recordings— the antennal olfactory sensitivity of virgin, mated, male, female, lab-reared and wild C. capitata following stimulation with fruit and leaf headspace of some host-plants: clementine, orange, prickly pear, lemon and apple. The results show that: (a) lab-reared mated males are more sensitive to host-plant fruit and leaf headspace than females, while the opposite was true for wild insects; (b) antennae of wild virgin males were more sensitive than the mated ones, while no difference was observed among lab-reared medflies; (c) lab-reared virgin females were more sensitive than mated ones, while few differences were found within wild medflies; (d) in mated insects, lab-reared males were more sensitive to both host-plant fruits and leaves than the wild ones, while the opposite was found for females. Taken together, these results show that the olfactory sensitivity to host-plant odors differs between virgin and mated and lab-reared and wild flies.
Reporting Standards for a Bland–Altman Agreement Analysis: A Review of Methodological Reviews
Authors: Gerke Oke
Published: May 2020

The Bland–Altman Limits of Agreement is a popular and widespread means of analyzing the agreement of two methods, instruments, or raters in quantitative outcomes. An agreement analysis could be reported as a stand-alone research article but it is more often conducted as a minor quality assurance project in a subgroup of patients, as a part of a larger diagnostic accuracy study, clinical trial, or epidemiological survey. Consequently, such an analysis is often limited to brief descriptions in the main report. Therefore, in several medical fields, it has been recommended to report specific items related to the Bland–Altman analysis. The present study aimed to identify the most comprehensive and appropriate list of items for such an analysis. Seven proposals were identified from a MEDLINE/PubMed search, three of which were derived by reviewing anesthesia journals. Broad consensus was seen for the a priori establishment of acceptability benchmarks, estimation of repeatability of measurements, description of the data structure, visual assessment of the normality and homogeneity assumption, and plotting and numerically reporting both bias and the Bland–Altman Limits of Agreement, including respective 95% confidence intervals. Abu-Arafeh et al. provided the most comprehensive and prudent list, identifying 13 key items for reporting (Br. J. Anaesth. 2016, 117, 569–575). An exemplification with interrater data from a local study accentuated the straightforwardness of transparent reporting of the Bland–Altman analysis. The 13 key items should be applied by researchers, journal editors, and reviewers in the future, to increase the quality of reporting Bland–Altman agreement analyses.
Cryptanalysis and Improvement of a Chaotic Map-Based Image Encryption System Using Both Plaintext Related Permutation and Diffusion
Authors: Lin Cheng-Yi , Wu Ja-Ling
Published: May 2020

In theory, high key and high plaintext sensitivities are a must for a cryptosystem to resist the chosen/known plaintext and the differential attacks. High plaintext sensitivity can be achieved by ensuring that each encrypted result is plaintext-dependent. In this work, we make detailed cryptanalysis on a published chaotic map-based image encryption system, where the encryption process is plaintext Image dependent. We show that some designing flaws make the published cryptosystem vulnerable to chosen-plaintext attack, and we then proposed an enhanced algorithm to overcome those flaws.
“Face-to-Face Trumps Everything”: An Exploration of Tutor Perceptions, Beliefs and Practice Within Blended Learning Environments
Authors: Youde Andrew
Published: May 2020

This paper explores the practices of higher education tutors in blended learning contexts. Significantly, the influence of their perceptions on practice was considered by investigating previous teaching and learning experiences, and their views of the affordances blended learning offers adult learners. The analysis was undertaken in relation to these learners’ perceptions of their tutors whilst studying part-time, vocationally relevant degrees, at a distance. A mixed methods approach was adopted to conduct a detailed exploration of eight tutors’ practice. Data analysis suggested that all tutors had negative experiences of online learning as students with these perceptions appearing to influence their practice. They generally avoided online pedagogies and adopted alternative approaches to their practice, namely a focus on face-to-face delivery with enhanced learner support, which was found to align with their described pedagogical beliefs. These tutors considered online teaching and learning as a deficit in this context.
Design of a Projectile-Borne Data Recorder Triggered by Overload
Authors: Wu Zhiqiang , Wang Yu , Zhu Lihua , Yang Fan
Published: May 2020

The projectile-borne data recorder is used to measure and record the position and attitude data of exterior ballistic flights, which experience high overload during the launching process. The navigation algorithm can be optimized by analyzing the data stored in the recorder. As the primary means of acquiring the navigation data, the micro inertial measurement unit (MIMU) is an inevitable part of the projectile equipment. However, its mechanical structure could hardly bear the high overload during operation. In view of the above problems, a novel projectile-borne data recorder triggered by overload is designed in this paper. The recorder could have the navigation system powered when the projectile leaves the barrel so as to activate the data recording of the high overload. Furthermore, the viability of the MIMU of the high overload is guaranteed through a specific way of system encapsulation. In the proposed design, the overload switch redundancy and the power supply redundancy were adopted to improve reliability. The proposed design is tested with practical experiments, and the results show that the proposed recorder could be effectively triggered by overloading, and the supply voltage is stable, which helps record reliable data for the projectile.
BBR-CWS: Improving the Inter-Protocol Fairness of BBR
Authors: Song Yeong-Jun , Kim Geon-Hwan , Cho You-Ze
Published: May 2020

TCP congestion control adjusts the sending rate in order to protect Internet from the continuous traffic and ensure fair coexistence among multiple flows. Especially, loss-based congestion control algorithms were mainly used, which worked relatively well for past Internet with low bandwidth and small bottleneck buffer size. However, the modern Internet uses considerably more sophisticated network equipment and advanced transmission technologies, and loss-based congestion control can cause performance degradation due to excessive queueing delay and packet loss. Therefore, Google introduced a new congestion control in 2016, Bottleneck Bandwidth Round-trip propagation time (BBR). In contrast with traditional congestion control, BBR tries to operate at the Kleinrock’s optimal operating point, where delivery rate is maximized and latency is minimized. However, when BBR and loss-based congestion control algorithms coexist on the same bottleneck link, most of bottleneck bandwidth is occupied by flows that use a particular algorithm, and excessive packet retransmission can occur. Therefore, this paper proposes a BBR congestion window scaling (BBR-CWS) scheme to improve BBR’s inter-protocol fairness with a loss-based congestion control algorithm. Through Mininet experiment results, we confirmed that fairness between BBR-CWS and CUBIC improved up to 73% and has the value of 0.9 or higher in most bottleneck buffer environments. Moreover, the number of packet retransmissions was reduced by up to 96%, compared to the original BBR.
Optimized Lossless Embedded Compression for Mobile Multimedia Applications
Authors: Yoon Sungchul , Jun Sungho , Cho Yongkwon , Lee Kilwhan , Jang Hyukjae , Han Tae Hee
Published: May 2020

Power consumption is a critical design factor in modern mobile chip design, in which the memory system with dynamic random-access memory (DRAM) consumes more than half of the entire system’s power. Without DRAM bandwidth compression, extreme multimedia operations such as 8K high dynamic range (HDR) recording and 8K video conference calling are not possible without sacrificing image quality or trimming because of thermal limitations or battery time sustainability constraints. Since heterogeneous processors are substantially involved in managing various types of fallbacks or software solutions, complicated compression algorithms for high-compression ratios are not actually adaptable owing to timing closure problems or high throughput requirements. In this paper, we propose evaluation metrics to assess lossless embedded compression (LEC) algorithms to reflect realistic design considerations for mobile multimedia scenarios. Furthermore, we introduce an optimized LEC implementation for contemporary multimedia applications in mobile devices based on the proposed metrics. The proposed LEC implementation enhances the compression ratio of LEC algorithms in other commercial application processors for contemporary premium smartphones by up to 9.2% on average, while maintaining the same timing closure condition.
Continuous Gesture Recognition Based on Time Sequence Fusion Using MIMO Radar Sensor and Deep Learning
Authors: Lei Wentai , Jiang Xinyue , Xu Long , Luo Jiabin , Xu Mengdi , Hou Feifei
Published: May 2020

Gesture recognition that is based on high-resolution radar has progressively developed in human-computer interaction field. In a radar recognition-based system, it is challenging to recognize various gesture types because of the lacking of gesture transversal feature. In this paper, we propose an integrated gesture recognition system that is based on frequency modulated continuous wave MIMO radar combined with deep learning network for gesture recognition. First, a pre-processing algorithm, which consists of the windowed fast Fourier transform and the intermediate-frequency signal band-pass-filter (IF-BPF), is applied to obtain improved Range Doppler Map. A range FFT based MUSIC (RFBM) two-dimensional (2D) joint super-resolution estimation algorithm is proposed to obtain a Range Azimuth Map to obtain gesture transversal feature. Range Doppler Map and Range Azimuth Map then respectively form a Range Doppler Map Time Sequence (RDMTS) and a Range Azimuth Map Time Sequence (RAMTS) in gesture recording duration. Finally, a Dual stream three-dimensional (3D) Convolution Neural Network combined with Long Short Term Memory (DS-3DCNN-LSTM) network is designed to extract and fuse features from both RDMTS and RAMTS, and then classify gestures with radial and transversal change. The experimental results show that the proposed system could distinguish 10 types of gestures containing transversal and radial motions with an average accuracy of 97.66%.
Design of a Low Power 10-b 8-MS/s Asynchronous SAR ADC with On-Chip Reference Voltage Generator
Authors: Shehzad Khuram , Verma Deeksha , Khan Danial , Ain Qurat , Basim Muhammad , Kim Sung , Pu YoungGun , Hwang Keum , Yang Youngoo , Lee Kang-Yoon
Published: May 2020

This paper presents an energy-efficient low power 10-b 8-MS/s asynchronous successive approximation register (SAR) analog-to-digital (ADC) converter. An inverted common-mode charge recovery technique is proposed to reduce the switching energy and to improve the linearity of the digital-to-analog converter (DAC). The proposed switching technique consumes only 149 CVREF2 switching energy for the 10-bit case. A rail-to-rail dynamic latch comparator is implemented with adaptive power control for better power efficiency. Additionally, to optimize the power consumption and performance of the logic part, a modified asynchronous type SAR control logic with digitally controllable delay cells is adopted. An on-chip reference voltage generator is also designed with an ADC core for practical use. The structure is realized using 55-nm complementary metal–oxide–semiconductor (CMOS) process technology. The proposed architecture achieves an effective number of bits (ENOB) of 9.56 bits and a signal-to-noise and distortion ratio (SNDR) level of 59.3 dB with a sampling rate of 8 MS/s at measurement level. The whole architecture consumes only 572 µW power when a power supply of 1 V is applied.
Numerical and Circuit Modeling of the Low-Power Periodic WPT Systems
Authors: Steckiewicz Adam , Stankiewicz Jacek Maciej , Choroszucho Agnieszka
Published: May 2020

This article presents a method for analysis of the low-power periodic Wireless Power Transfer (WPT) system, using field and circuit models. A three-dimensional numerical model of multi-segment charging system, with periodic boundary conditions and current sheet approximation was solved by using the finite element method (FEM) and discussed. An equivalent circuit model of periodic WPT system was proposed, and required lumped parameters were obtained, utilizing analytical formulae. Mathematical formulations were complemented by analysis of some geometrical variants, where transmitting and receiving coils with different sizes and numbers of turns were considered. The results indicated that the proposed circuit model was able to achieve similar accuracy as the numerical model. However, the complexity of model and analysis were significantly reduced.
Analysis of Air Permeability of Insulated Masonry Walls
Authors: Geležiūnas Valdemaras , Banionis Karolis , Bliūdžius Raimondas , Paukštys Valdas , Kumžienė Jurga
Published: May 2020

Recently, the construction of external ventilated walls has become popular for public and office buildings. These blocks are used without internal rendering because of their good interior surface, stable dimensions and various filling of masonry joints, which provide an attractive architectural appearance. However, problems with the airtightness of such walls often occur. Currently, there are no standard methods to predict the airtightness of such wall. In practice, samples of particular walls are produced, and their air permeability is measured at laboratories. For the broader use of the results of laboratory air permeability measurements, a methodology has been developed to predict the air permeability of block masonry walls using experimentally determined air flow resistances of the individual layers. The masonry from various blocks were used for the research; mineral wool boards of various air permeability were used for thermal insulation and the wind protection layer. After measuring the air resistance of the samples, the air flow resistances of walls of different construction were calculated. This study compared the calculated and measured air permeability values of different wall masonry samples and evaluated the suitability of created calculation method for prediction of the airtightness of insulated block masonry wall.
A Study on the V2G Technology Incorporation in a DC Nanogrid and on the Provision of Voltage Regulation to the Power Grid
Authors: Skouros Ioannis , Karlis Athanasios
Published: May 2020

Currently, environmental and climate change issues raise a lot of concerns related to conventional vehicles and renewable energy generation methods. Thus, more and more researchers around the world focus on the development and deployment of Renewable Energy Sources (RES). Additionally, due to the technological advancements in power electronics and electrical batteries, Electrical Vehicles (EVs) are becoming more and more popular. In addition, according to the Vehicle-to-Grid (V2G) operation, the EV batteries can provide electrical energy to the power grid. In this way, many ancillary services can be provided. A Direct Current (DC) nanogrid can be composed by combining the aforementioned technologies. Nanogrids present high efficiency and provide a simple interaction with renewable energy sources and energy storage devices. Firstly, the present study describes the design considerations of a DC nanogrid as well as the control strategies that have to be applied in order to make the V2G operation feasible. Furthermore, the provision of voltage regulation toward the power grid is investigated though the bidirectional transfer of active and reactive power between the DC nanogrid and the power grid. Afterwards, the voltage regulation techniques are applied in an Alternating Current (AC) radial distribution grid are investigated. The proposed system is simulated in Matlab/Simulink software and though the simulation scenarios the impact of the voltage regulation provided by the DC nanogrid is investigated.
An Extended Grid Diagram for Heat Exchanger Network Retrofit Considering Heat Exchanger Types
Authors: Wang Bohong , Klemeš Jiří Jaromír , Varbanov Petar Sabev , Zeng Min
Published: May 2020

Heat exchanger network (HEN) retrofit is a vital task in the process design to improve energy savings. Various types of heat exchangers such as shell and tube, double-pipe, compact plate, and spiral tube have their working temperature ranges and costs. Selecting suitable types of heat exchangers according to their temperature ranges and costs is a crucial aspect of industrial implementation. However, considering the type of heat exchangers in the HEN retrofit process is rarely seen in previous publications. This issue can be solved by the proposed Shifted Retrofit Thermodynamic Grid Diagram with the Shifted Temperature Range of Heat Exchangers (SRTGD-STR). The temperature ranges of six widely used heat exchanger types are coupled in the grid diagram. This diagram enables the visualisation of identifying the potential retrofit plan of HEN with heat-exchanger type selection. The retrofit design aims to minimise utility cost and capital cost. An illustrative example and a case study are presented to show the effectiveness of the method.
Centralized and Decentralized Recycle Policy with Transboundary Pollution
Authors: Lee Chien-Hui , Ko Po-Sheng , Wang Yu-Lin , Lee Jen-Yao , Kwo Jiong-Hung
Published: May 2020

In this study, under the existence of unilateral cross-border environmental pollution in two regions, a complete information dynamic game theory is constructed to discuss the environmental policy (recycling fee and treatment subsidy) formulation of the central government by two local governments. As a result, it was found that the spillover effect will reduce the level of social welfare. At the same time, the intervention of the central government and the adoption of policies tailored to local conditions will be conducive to the improvement of social welfare.
Regeneration Dynamics Following the Formation of Understory Gaps in a Slovakian Beech Virgin Forest
Authors: Feldmann Eike , Glatthorn Jonas , Ammer Christian , Leuschner Christoph
Published: May 2020

The frequency and size of canopy gaps largely determine light transmission to lower canopy strata, controlling structuring processes in the understory. However, quantitative data from temperate virgin forests on the structure of regeneration in gaps and its dynamics over time are scarce. We studied the structure and height growth of tree regeneration by means of sapling density, shoot length growth and cumulative biomass in 17 understory gaps (29 to 931 m2 in size) in a Slovakian beech (Fagus sylvatica L.) virgin forest, and compared the gaps with the regeneration under closed-canopy conditions. Spatial differences in regeneration structure and growth rate within a gap and in the gap periphery were analyzed for their dependence on the relative intensities of direct and diffuse radiation (high vs. low). We tested the hypotheses that (i) the density and cumulative biomass of saplings are higher in gaps than in closed-canopy patches, (ii) the position in a gap influences the density and height growth of saplings, and (iii) height growth of saplings increases with gap size. Sapling density and biomass were significantly higher in understory gaps than under closed canopy. Density of saplings was positively affected by comparatively high direct, but low diffuse radiation, resulting in pronounced spatial differences. In contrast, sapling shoot length growth was positively affected by higher levels of diffuse radiation and also depended on sapling size, while direct radiation intensity was not influential. Conclusively, in this forest, regeneration likely becomes suppressed after a short period by lateral canopy expansion in small gaps (<100 m2), resulting in a heterogeneous understory structure. In larger gaps (≥100 m2) saplings may be capable even at low plant densities to fill the gap, often forming a cohort-like regeneration layer. Thus, gaps of different sizes imprint on the resulting canopy structure in different ways, enhancing spatial heterogeneity.
National Set of MAES Indicators in Greece: Ecosystem Services and Management Implications
Authors: Kokkoris Ioannis P. , Mallinis Georgios , Bekri Eleni S. , Vlami Vassiliki , Zogaris Stamatis , Chrysafis Irene , Mitsopoulos Ioannis , Dimopoulos Panayotis
Published: May 2020

Research Highlights: The developed National Set of Indicators for the Mapping and Assessment of Ecosystems and their Services (MAES) implementation in Greece at the national level sets the official, national basis on which future studies will be conducted for MAES reporting for the achievement of targets within the National and the European Union (EU) biodiversity Strategy. Background and Objectives: Greece is currently developing and implementing a MAES nation-wide program based on the region’s unique characteristics following the proposed methodologies by the European Commission, in the frame of the LIFE-IP 4 NATURA project (Integrated actions for the conservation and management of Natura 2000 sites, species, habitats and ecosystems in Greece). In this paper, we present the steps followed to compile standardized MAES indicators for Greece that include: (a) collection and review of the available MAES-related datasets, (b) shortcomings and limitations encountered and overcome, (c) identification of data gaps and (d) assumptions and framework setting. Correspondence to EU and National Strategies and Policies are also examined to provide an initial guidance for detailed thematic studies. Materials and Methods: We followed the requirements of the EU MAES framework for ecosystem services and ecosystem condition indicator selection. Ecosystem services reported under the selected indicators were assigned following the Common International Classification of Ecosystem Services. Spatial analysis techniques were applied to create relevant thematic maps. Results: A set of 40 MAES indicators was drafted, distributed in six general indicator groups, i.e., Biodiversity, Environmental quality, Food, material and energy, Forestry, Recreation and Water resources. The protocols for the development and implementation of an indicator were also drafted and adopted for future MAES studies in Greece, providing guidance for adaptive development and adding extra indicators when and where needed. Thematic maps representing ecosystem services (ES) bundles and ES hotspots were also created to identify areas of ES importance and simultaneously communicate the results at the national and regional levels.
Mechanisms by Which Pre-Commercial Thinning Increases Black Spruce Growth in Different Climates and Soil Types
Authors: Wotherspoon Amy , Bradley Robert L. , Houle Daniel , Tremblay Stéphane , Barrette Martin , Reicis Krista
Published: May 2020

In the province of Québec (Canada), pre-commercial thinning (PCT) is a common silvicultural practice applied to young black spruce (Picea mariana (Mill.) BSP) stands. PCT removes some of the competing vegetation and smaller black spruce stems, in order to improve growth rates and reduce forest rotation intervals. It is uncertain whether this positive response in black spruce growth is primarily due to lower competition for resources or to other mechanisms, which may vary according to climate or edaphic conditions. We sampled soils and black spruce needles in PCT-treated and non-treated control plots occurring in two climate regimes, as well as on two contrasting soil parent materials within one of these two climate regimes (i.e., three “site types”). We performed our sampling approximately 20 years after treatment. Paired treatment plots (i.e., PCT vs. control) were replicated at four independent sites in each of the three site types, for a total of 24 plots. Over two consecutive years, we measured stand structural characteristics, indices of soil N fertility, soil microbial activity, indices of soil moisture availability, canopy openness, and foliar characteristics in each plot. In each site type, PCT decreased total basal area but increased radial growth of individual trees. Across all plots, soil N mineralization rates measured in 2016 were positively related to foliar N concentrations of one-year-old needles collected in 2017. Annual precipitation, drainage class, potential evapotranspiration, and climate moisture index all indicated that plots occurring in the drier climate and on glacial till deposits were more prone to summer moisture deficits. Accordingly, PCT increased forest floor moisture only in this site type, which may benefit tree growth. In the wetter climate and on poorly drained soils, however, we found evidence that PCT reduces soil N fertility, presumably by increasing the spread of ericaceous shrubs in the understory. In the dry fertile site type, the range in canopy openness was substantially higher (12–37%) and correlated negatively with tree diameter, suggesting that greater light availability did not improve tree growth. Taken collectively, our data suggest that PCT increases black spruce growth across a broad range of site conditions found in Québec, presumably by lowering intraspecific competition for resources. However, on drier sites, PCT may also benefit trees by increasing soil moisture availability, whereas wetter climates may mitigate the beneficial effect of PCT due to a loss of soil N fertility.