bluuebunny/crossref_metadata_2025 huggingface dataset:
Multiple Antimicrobial Resistance and Heavy Metal Tolerance of Biofilm-Producing Bacteria Isolated from Dairy and Non-Dairy Food Products
Authors: Ejaz Hasan , Junaid Kashaf , Yasmeen Humaira , Naseer Amina , Alam Hafsa , Younas Sonia , Qamar Muhammad Usman , Abdalla Abualgasim E. , Abosalif Khalid O. A. , Ahmad Naveed , Bukhari Syed Nasir Abbas
Published: September 2022

Foodborne pathogens have acquired the ability to produce biofilms to survive in hostile environments. This study evaluated biofilm formation, antimicrobial resistance (AMR), and heavy metal tolerance of bacteria isolated from dairy and non-dairy food products. We aseptically collected and processed 200 dairy and non-dairy food specimens in peptone broth, incubated them overnight at 37 °C, and sub-cultured them on various culture media. Bacterial growth was identified with biochemical tests and API 20E and 20NE strips. The AMR of the isolates was observed against different antibacterial drug classes. Biofilm formation was detected with the crystal violet tube method. Heavy metal salts were used at concentrations of 250–1500 µg/100 mL to observe heavy metal tolerance. We isolated 180 (50.4%) bacteria from dairy and 177 (49.6%) from non-dairy food samples. The average colony-forming unit (CFU) count for dairy and non-dairy samples was 2.9 ± 0.9 log CFU/mL and 5.1 ± 0.3 log CFU/mL, respectively. Corynebacterium kutscheri (n = 74), lactobacilli (n = 73), and Staphylococcus aureus (n = 56) were the predominant Gram-positive and Shigella (n = 10) the predominant Gram-negative bacteria isolated. The correlation between biofilm formation and AMR was significant (p < 0.05) for most cephalosporins, aminoglycosides, and fluoroquinolones. Heavy metal tolerance tended to be higher in biofilm producers at different metal concentrations. The pathogens isolated from dairy and non-dairy food showed a high burden of AMR, high propensity for biofilm formation, and heavy metal tolerance, and pose an imminent threat to public health.
Assessment of Chitosan Coating Enriched with Free and Nanoencapsulated Satureja montana L. Essential Oil as a Novel Tool for Beef Preservation
Authors: Đorđević Natalija , Karabegović Ivana , Cvetković Dragoljub , Šojić Branislav , Savić Dragiša , Danilović Bojana
Published: September 2022

The effect of chitosan coating enriched with free and nanoencapsulated Satureja montana L. essential oil (EO) on microbial, antioxidant and sensory characteristics of beef was analyzed. Different concentrations of free Satureja montana L. EO (SMEO) and nanoparticles (CNPs) were added to chitosan coatings, namely 0.25%, 0.5% and 1%. The beef samples were immersed in the chitosan coatings and stored at +4 °C for 20 days. In this period, the changes in pH value, total viable count (TVC), lactic acid bacteria, psychrophilic bacteria and Pseudomonas spp. were analyzed. The lipid oxidation of beef was determined by the TBAR assay, while sensory analysis was performed by means of the descriptive evaluation method. Generally, the influence of chitosan coating with CNPs on the growth of the tested microorganisms was more pronounced compared to SMEO. Treatment with coating enriched with 1% CNPs resulted in the reduction in TVC and Pseudomonas spp. by 2.4 and 3 log CFU/g, compared to the control, respectively. Additionally, all applied coatings with SMEO and CNPs resulted in the prolonged oxidative stability of the meat The addition of free SMEO created an unnatural aroma for the evaluators, while this odor was neutralized by nanoencapsulation. The durability of color, smell and general acceptability of beef was significantly increased by application of chitosane coatings with the addition of SMEO or SMEO-CNPs, compared to the control. This research indicates the potential application of enriched chitosan coatings in beef preservation in order to improve meat safety and prolong shelf-life.
Trigeminal Stimulus Menthol Masks Bitter Off-Flavor of Artificial Sweetener Acesulfame-K
Authors: Büchner Kai , Haagen Jana , Sastrosubroto Ashtri , Kerpes Roland , Freiherr Jessica , Becker Thomas
Published: September 2022

Consumer health concerns and regulatory policies lead to a growing demand for sugar-sweetened beverage alternatives. A reduced energy content can be achieved using artificial sweeteners, which often also convey a metallic or bitter off-flavor. Therefore, the alteration of sweetness perception and masking of potential off-flavors are paramount for improving sweet beverages. Trigeminal stimuli, such as capsaicin (spicy) or menthol (cooling), have been used to influence taste perception in food items, although their use in beverages has not yet been systematically investigated. Here, the influence of menthol on sweetness perception in an aqueous solution is examined both on the sensory and psychophysiological level. The addition of menthol had no sensory effect on sweetness perception; however, psychophysiological measurements suggest a boost in the physiological response to cold perception through the addition of sugar. Moreover, menthol addition shifted the recognition threshold of unpleasant bitterness of the sweetener acesulfame-K from 21.35 to 36.93 mg/L, masking the off-flavor. These findings illuminate the complexity of trigeminal perception influences on taste. Further investigation of these effects can render trigeminal stimuli an effective tool to enhance beverage aroma and flavor.
Bioactive Compounds and Quality Evaluation of Red-Pigmented Rice Processed by Germination and Roasting
Authors: Kang Soo-Ji , Jeong Su-Yeon , Islam Mohammad Zahirul , Shin Bo-Kyung , Park Young Jin , Kim Jae Kwang , Lee Young-Tack , Lee Jong-Hun
Published: September 2022

Red-pigmented rice was germinated and processed to develop germinated red rice tea, and the changes in physicochemical, bioactive, and microbial properties due to germination and roasting were investigated. The moisture and crude ash contents of red rice decreased after germination and roasting. Crude protein and crude fat contents increased after germination but slightly decreased after roasting. Total phenolics, flavonoids, and antioxidant activities (DPPH and ABTS radical scavenging activities) increased following germination and heat treatment. However, the increased levels of γ-amino butyric acid after germination significantly decreased during the subsequent roasting step. In addition, total bacteria, yeast, and mold counts increased during the germination process but decreased after heat treatment as compared to those in the original grain; Escherichia coli was not detected. Therefore, germination and subsequent roasting could effectively enhance the contents of the most bioactive compounds and maintain microbial stability in red-pigmented rice.
Presence of Chlorate and Perchlorate Residues in Raw Bovine Milk from Italian Farms
Authors: Nobile Maria , Danesi Luigi , Pavlovic Radmila , Mosconi Giacomo , Di Cesare Federica , Arioli Francesco , Villa Roberto , Chiesa Luca Maria , Panseri Sara
Published: September 2022

Chlorates and perchlorates are endocrine disruptors and emerging environmental contaminants found in various types of foods, including milk and dairy products. The presence of chlorate has been associated with the use of chlorine-based sanitizers to disinfect equipment and water used in food processing. Perchlorate, on the other hand, occurs naturally in the environment but is also released from anthropogenic sources. To protect consumers, the EU set an MRL for chlorate in milk but not for perchlorate. Considering that data on chlorates and perchlorates in this field are limited, the objective of this study was to assess the presence of these two anions in 148 samples of raw bovine milk collected from different farms of Lombardy and grouped in three different geographical zones. Chlorate was detected in 73% of the samples analyzed, at concentrations ranging from <LOQ to 18.70 μg kg−1 with an average value of 7.10 ± 5.88 μg kg−1 below the MRL; perchlorate with a frequency of 99%, in the range from <LOQ to 6.95 μg kg−1 and an average value of 4.06 ± 1.58 μg kg−1. No significant differences were detected among the three geographical zones. An evaluation of the estimated daily intake of perchlorate through milk confirmed the absence of risk for Italian consumers.
Fed-Batch Fermentation of Saccharomyces pastorianus with High Ribonucleic Acid Yield
Authors: Chen Hao , Wang Jinjing , Li Qi , Xu Xin , Niu Chengtuo , Zheng Feiyun , Liu Chunfeng
Published: September 2022

(1) Background: The degradation products of ribonucleic acid (RNA)are widely used in the food and pharmaceutical industry for their flavoring and nutritional enhancement functions. Yeast is the main source for commercial RNA production, and an efficient strain is the key to reducing production costs; (2) Methods: A mutant Saccharomyces pastorianus G03H8 with a high RNA yield was developed via ARTP mutagenesis and fed-batch fermentation was applied to optimize production capacity. Genome sequencing analysis was used to reveal the underlying mechanism of higher RNA production genetic differences in the preferred mutant; (3) Results: Compared with the highest RNA content of the mutant strain, G03H8 increased by 40% compared with the parental strain G03 after response surface model optimization. Meanwhile, in fed-batch fermentation, G03H8′s dry cell weight (DCW) reached 60.58 g/L in 5 L fermenter by molasses flowing and RNA production reached up to 3.58 g/L. Genome sequencing showed that the ribosome biogenesis, yeast meiosis, RNA transport, and longevity regulating pathway were closely related to the metabolism of high RNA production; (4) Conclusion: S. pastorianus G03H8 was developed for RNA production and had the potential to greatly reduce the cost of RNA production and shorten the fermentation cycle. This work lays the foundation for efficient RNA content using S. pastorianus.
Assessment of Personal Rapid Transit System Configurations Regarding Efficiency and Service Quality
Authors: Gotzler Felix , Neumann Franziska , Adenaw Lennart
Published: September 2022

In order to transform cities into more liveable, safe, and sustainable places, we must shift our mobility paradigms. As one auspicious concept amongst novel intelligent transportation systems, personal rapid transit (PRT) disaggregates urban transportation into small, electric vessels that are centrally operated on dedicated infrastructure, yielding the potential to make public transit more convenient, affordable, and sustainable all at once. In light of this, we examined the potential performance of PRT in a medium-sized German city. Utilizing the traffic simulator SUMO, as well as a specifically developed open source mobility scenario consisting of infrastructure and travel demand, we assessed the level of service and efficiency. We found that a fleet of 30 vehicles can serve the mobility demand of the chosen city while passenger waiting times are guaranteed to stay below three minutes. Vehicle occupancies can be doubled when coordinating vehicles between stations instead of letting them idle randomly. Furthermore, our results show that different combinations of system designs and operating strategies succeed in meeting typical performance requirements—for instance, an operating strategy where unoccupied vehicles idle randomly can effectively compensate for a reduced fleetsize. Depending on the preliminaries of specific cities, such as the availability of space, travel behavior, political background, or acceptable investment and operational costs, a matching transportation system can be designed around the quantitative findings obtained in this study.
Social Learning between Groups: Imitation and the Role of Experience
Authors: Schlag Karl H.
Published: September 2022

Social learning often occurs between groups with different levels of experience. Yet little is known about the ideal behavioral rules in such contexts. Existing insights only apply when individuals learn from each other in the same group. In this paper, we close this gap and consider two groups, novices and experienced. Experienced should not learn from novices. For novices learning from experienced, a particular form of probabilistic imitation is selected. Novices should imitate any experienced who is more successful, and sometimes but not always imitate an experienced who is less successful.
Labor Market Integration of High-Skilled Immigrants in Canada: Employment Patterns of International Medical Graduates in Alternative Jobs
Authors: Turin Tanvir C. , Chowdhury Nashit , Lake Deidre , Chowdhury Mohammad Z. I.
Published: September 2022

Background: International medical graduates (IMGs) in Canada are individuals who received their medical education and training outside Canada. They undergo a complex licensing procedure in their host country and compete for limited opportunities available to become practicing physicians. Many of them cannot succeed or do not have the resources or interest to undergo this complex and unpredictable career pathway and seek alternative career options. In this study, we aimed to understand how IMGs integrate into the alternative job market, their demographic characteristics, and the types of jobs they undertake after moving to Canada. Methods: An anonymous cross-sectional, online, nationwide, and open survey was conducted among IMGs in Canada. In addition to demographic information, the questionnaire included information on employment status, types of jobs, professional experience, and level of medical education and practice (e.g., specialties, subspecialties, etc.). We conducted a survey of 1740 IMGs in total; however, we excluded responses from those IMGs who are currently working in a clinical setting, thus limiting the number of responses to 1497. Results: Of the respondents, 43.19% were employed and 56.81% were unemployed. Employed participants were more likely to be older males, have stayed longer in Canada, and had more senior-level job experience before moving to Canada. We also observed that the more years that had passed after graduation, the higher the likelihood of being employed. The majority of the IMGs were employed in health-related nonregulated jobs (50.45%). The results were consistent across other demographic characteristics, including different provinces, countries of origin, gender, time since graduation, and length of stay in Canada. Conclusions: This study found that certain groups of IMGs, such as young females, recent immigrants, recent graduates, and less experienced IMGs had a higher likelihood of being unemployed. These findings will inform policymakers, immigrant and professional service organizations, and researchers working for human resources and professional integration of skilled migrants to develop programs and improve policies to facilitate the employment of IMGs through alternative careers.
Genome-Based Medicine for Acute Myeloid Leukemia: Study and Targeting of Molecular Alterations and Use of Minimal Residual Disease as a Biomarker
Authors: Testa Ugo , Castelli Germana , Pelosi Elvira
Published: September 2022

Acute myeloid leukemia (AML) is a highly heterogeneous hematologic malignancy characterized by the clonal proliferation of hematopoietic stem and progenitor cells (HSPCs) and blockade of differentiation and proliferation of immature myeloid cells that accumulate in bone marrow at the expense of normal hematopoiesis. AMLs originate from the expansion of HSPCs progressively acquiring somatic mutations. The development of high-throughput sequencing techniques has helped to discover the genetic heterogeneity and complexity of AMLs, revise diagnostic and prognostic criteria, and to identify new therapeutic targets. These studies have allowed the identification of several recurrent driver mutations and the definition of a rational molecular classification of these tumors. In parallel, the development of techniques for the determination of single-cell mutational profiling has considerably contributed to understanding the clonal heterogeneity and evolution of AMLs. The acquisition of these genetic data coupled with the identification of molecular therapeutic targets has determined a considerable expansion of the therapeutic armamentarium, with the development of several new drugs highly active against specific AML subtypes. These developments have increased the interest and the need for sensitive techniques for the identification of minimal residual disease, the population of leukemia cells that survives despite morphological remission and causes disease relapse.
Associations between Schizotypal Facets and Symptoms of Disordered Eating in Women
Authors: Swami Viren , Barron David , Furnham Adrian
Published: September 2022

Research has suggested that schizotypy—a personality organisation representing latent vulnerability for schizophrenia-spectrum disorders—may be elevated in women with symptoms of disordered eating. However, studies have not fully considered associations between symptoms of disordered eating and multidimensional schizotypy. To overcome this limitation, we asked an online sample of 235 women from the United States to complete measures of symptoms of disordered eating (drive for thinness, body dissatisfaction, and bulimic symptoms) and multidimensional schizotypy. Correlational analyses indicated significant associations between drive for thinness and bulimic symptoms, respectively, and most schizotypal facets. Body dissatisfaction was significantly associated with only two schizotypal facets. Overall, the strength of correlations was weak-to-moderate. Regression results indicated that only the schizotypal feature of excessive social anxiety was significantly associated with all risk for disordered eating factors. These results are consistent with aetiological models of disordered eating that highlight socio-affective difficulties as risk factors for symptoms of disordered eating.
How Do Elite Female Athletes Cope with Symptoms of Their Premenstrual Period? A Study on Rugby Union and Football Players’ Perceived Physical Ability and Well-Being
Authors: Modena Roberto , Bisagno Elisa , Schena Federico , Carazzato Simone , Vitali Francesca
Published: September 2022

Women’s participation in sports has recently grown worldwide, including in sports typically associated with men (e.g., rugby and football). Similarly, literature on female athletes has increased, but how they cope with premenstrual (PM) physical and affective symptoms remains a poorly studied topic. Our study aimed to explain which coping strategies elite female rugby and football players use during their PM period to maintain perceived physical ability (PPA) and well-being. A mediation model analysis considering coping strategies (i.e., avoiding harm, awareness and acceptance, adjusting energy, self-care, and communicating) as independent variables, PPA and well-being as dependent variables, and PM physical and affective symptoms and PM cognitive resources as mediators was run on the data collected via an anonymous online survey. A dysfunctional impact of avoiding harm (indirect) and adjusting energy (both direct and indirect) and a functional indirect influence of awareness and acceptance, self-care, and communicating as coping strategies were found on PPA and well-being during the PM period. As predicted, PM physical and affective symptoms as mediators reduced PPA and well-being, while PM cognitive resources enhanced them. These results may inform practitioners on how to support elite female athletes’ PPA and well-being by knowing and reinforcing the most functional PM coping strategies for them.
Food Security: 3D Dynamic Display and Early Warning Platform Construction and Security Strategy
Authors: Sun Ning , Tang Sai , Zhang Ju , Wu Jiaxin , Wang Hongwei
Published: September 2022

Since it affects a nation’s economy and people’s wellbeing, food security is a crucial national security requirement. In order to realize multi-angle grain data presentation and analysis and achieve the goal of deep mining, we propose a 3D dynamic visualization analysis method of multidimensional agricultural spatial–temporal data based on the self-organizing map. This method realizes the multi-angle display and analysis of grain data and achieves the purpose of deep mining. With the outbreak of COVID-19, the global food security situation is not optimistic, so it is necessary to use the food security early warning system to solve the food security issue. Machine learning has emerged widely in recent years and has been applied in various fields. Therefore, it is an excellent way to solve food security to apply the model in machine learning to construct a food security early warning system. Afterward, a food security early warning platform is developed with a support vector regression (SVR) model to ensure food security. Finally, we analyze China’s medium and long-term food security policy in line with modernization objectives. The experimental results show that the food security early warning platform based on the SVR model from 2007 to 2016 is effective compared with the actual situation every year. Through analyses, we should improve the stability, reliability, and sustainability of food supply, firmly hold the food security initiative, and construct a national food security guarantee system matching the goal of modernization.
The Well-Being of Primary School Teachers during COVID-19
Authors: Sigursteinsdottir Hjordis , Rafnsdottir Gudbjorg Linda
Published: September 2022

This study examines the self-rated health and well-being of Icelandic teachers just before and over a year after COVID-19 first appeared. We ask, what was the stress level in 2021 compared to 2019 and the impact of mental and physical health and health symptoms on perceived stress? Were there any changes in self-assessed mental and physical health? Were there any changes in self-assessed mental and physical health symptoms? The study is based on an online survey conducted in 2019 and 2021. A total of 920 primary school teachers answered the questionnaire in part or in full, after three reminders. The main findings show increased stress, worsening mental and physical health, and increasing mental and physical symptoms in 2021 compared to 2019. The results also show a higher percentage of women than men reporting high stress, with women scoring higher on the PSS scale, but the gender patterns for mental and physical health are less clear. The results show that the COVID-19 pandemic had negative consequences on the health and well-being of the teachers. The study demonstrates the importance of school authorities keeping an exceptionally watchful eye on the welfare and well-being of teachers in the wake of the COVID-19 pandemic.
Effects of Group Emotion and Moral Belief on Pro-Environmental Behavior: The Mediating Role of Psychological Clustering
Authors: Li Shanshan , Chen Feiyu , Gu Xiao
Published: September 2022

An important topic in ecological environmental protection concerns the determination of a method to guide individual pro-environmental behavior from the dual perspectives of immediate intervention and long-term shaping. This research analyzed the influence of group emotions and moral beliefs on the pro-environmental behavior of residents and introduces the concept of the “psychological cluster” to explore the mediating effect of the “psychological cluster” on group emotions, moral beliefs, the pro-environmental behavior of residents, and its various dimensions. The results of a questionnaire survey, factor analysis, regression analysis, and other methods showed that group emotions and moral beliefs can significantly the predict pro-environmental behaviors of residents, and moral beliefs have a significant impact on basic pro-environmental behaviors. Each dimension of the psychological cluster has a significant effect on pro-environmental behavior. Among them, the psychological cluster has the most significant effect on the citizen-type pro-environmental behavior. That is to say, when individuals are passionate about environmental causes, and feel angry and guilty about damaging the environment, they will be encouraged to actively participate in environmental protection activities in public places and give advice on environmental protection. Group emotion and moral belief can drive the occurrence of a psychological cluster, which leads to the generation of group behavior. In other words, psychological clustering acts as a “middleman” between group emotion, moral belief, and pro-environmental behavior of residents. However, its influence is not completely mediated via the psychological cluster. In summary, the psychological cluster plays a partial mediating role in the relationship between group emotion, moral belief, and the pro-environmental behavior of residents. Finally, corresponding policy suggestions are put forward based on this research.
The Effect of Physical Exercise and Internet Use on Youth Subjective Well-Being—The Mediating Role of Life Satisfaction and the Moderating Effect of Social Mentality
Authors: Tao Baole , Chen Hanwen , Lu Tianci , Yan Jun
Published: September 2022

Youth subjective well-being is enhanced not only from physical exercise but also from internet use. Based on the 2017 China General Social Survey (CGSS) data, the Bootstrap method was used to examine the mechanisms of the effects of physical exercise and internet use on youth subjective well-being. In this study, the questionnaire data of 619 Chinese young people (18–35 years old) were selected as the sample source. It was found that physical exercise (2.881 ± 1.352) and internet use (4.544 ± 0.756) had positive effects on youth subjective well-being (88.762 ± 11.793). Life satisfaction (2.253 ± 0.826) partially mediated the development of physical exercise and internet use on subjective well-being, with indirect effects of 34.1% and 30.4%, respectively. A social mindset (10.181 ± 1.966) played a moderating role in the relationship between physical exercise and youth subjective well-being and internet use and youth subjective well-being in both groups. The positive effects of physical exercise and internet use on youth subjective well-being gradually increased with the improvement in social mindset. This study revealed the mechanisms of physical activity and internet use on subjective well-being and that life satisfaction and the social mindset of youth are essential factors influencing subjective well-being.
How Do Perceived Changes in Child and Adolescent Activities Relate to Perceptions of Health during COVID-19? Exploring Heterogeneity during the Pandemic
Authors: Gilbert Amanda S. , Jabbari Jason , Hernández Racquel
Published: September 2022

COVID-19 affected child/adolescent activities (e.g., extra-curricular, screen time), along with physical health (PH) and mental health (MH); however, less is known about the relationship between changes in activities and PH and MH in the United States and how these relationships vary by race/ethnicity. To address this gap, data were used from a national survey (Socio-Economic Impacts of COVID-19 Survey) administered May–June 2021 (n = 853). Multinomial logistic regression explored changes in outdoor, school, extracurricular, friend, and screen time activities with changes in PH and MH; interactions explored moderation by race/ethnicity. Results showed increases in outdoor (RRR 2.36, p = 0.003), school (RRR 3.07, p < 0.001), and extracurricular activities (RRR 3.05, p < 0.001), which were associated with increases in chances of better PH. Better MH was more likely for children/adolescents where friend activities (RRR 3.34, p < 0.001) and extracurriculars (RRR 4.48, p < 0.001) increased. Except for extracurriculars, heterogeneous relationships were observed (e.g., increases and decreases in activities were simultaneously related to better and worse health). The relationship between outdoor activities and screen time with health were moderated by race/ethnicity. Findings support facilitating outdoor, school, extracurricular, and friend activities, which were positively related to health. Given heterogeneity and variation by race/ethnicity, more research is needed to understand the complex relationship between activities and health during COVID-19.
Galactose in the Post-Weaning Diet Programs Improved Circulating Adiponectin Concentrations and Skeletal Muscle Insulin Signaling
Authors: Sun Peixin , Bouwman Lianne M. S. , de Deugd Jo-lene , van der Stelt Inge , Oosting Annemarie , Keijer Jaap , van Schothorst Evert M.
Published: September 2022

Short-term post-weaning nutrition can result in long-lasting effects in later life. Partial replacement of glucose by galactose in the post-weaning diet showed direct effects on liver inflammation. Here, we examined this program on body weight, body composition, and insulin sensitivity at the adult age. Three-week-old female C57BL/6JRccHsd mice were fed a diet with glucose plus galactose (GAL; 16 energy% (en%) each) or a control diet with glucose (GLU; 32 en%) for three weeks, and afterward, both groups were given the same high-fat diet (HFD). After five weeks on a HFD, an oral glucose tolerance test was performed. After nine weeks on a HFD, energy metabolism was assessed by indirect calorimetry, and fasted mice were sacrificed fifteen minutes after a glucose bolus, followed by serum and tissue analyses. Body weight and body composition were not different between the post-weaning dietary groups, during the post-weaning period, or the HFD period. Glucose tolerance and energy metabolism in adulthood were not affected by the post-weaning diet. Serum adiponectin concentrations were significantly higher (p = 0.02) in GAL mice while insulin, leptin, and insulin-like growth factor 1 concentrations were not affected. Expression of Adipoq mRNA was significantly higher in gonadal white adipose tissue (gWAT; p = 0.03), while its receptors in the liver and skeletal muscles remained unaffected. Irs2 expression was significantly lower in skeletal muscles (p = 0.01), but not in gWAT or Irs1 expression (in both tissues). Gene expressions of inflammatory markers in gWAT and the liver were also not affected. Conclusively, galactose in the post-weaning diet significantly improved circulating adiponectin concentrations and reduced skeletal muscle Irs2 expression in adulthood without alterations in fat mass, glucose tolerance, and inflammation.
Tumor-Promoting Actions of HNRNP A1 in HCC Are Associated with Cell Cycle, Mitochondrial Dynamics, and Necroptosis
Authors: Zhao Biao , Lv Xiaochen , Zhao Xiaoqi , Maimaitiaili Subinuer , Zhang Yuheng , Su Ke , Yu Hang , Liu Cheng , Qiao Tong
Published: September 2022

Hepatocellular carcinoma (HCC) is one of the most frequent malignancies in the world. Although increasing evidence supports the role of heterogeneous ribonucleoprotein particle A1 (HNRNP A1) in tumor progression, the function of HNRNP A1 in HCC remains unclear. Here, we focused on the role of HNRNP A1 in the development of HCC. In this study, we found HNRNP A1 participates in many aspects of HCC, such as progression and prognosis. Our results showed that HNRNP A1 is upregulated in human HCC tissues and cell lines. High expression of HNRNP A1 can promote the proliferation, migration, and invasion in HCC cells and accelerate tumor progression in mice. Moreover, we found that HNRNP A1 prevents the senescence process of HCC cells. Knocking down of HNRNP A1 promotes the expression of P16INK4, which arrests the cell cycle and then induces the senescence phenotype in HCC cells. Furthermore, we found that HNRNP A1 regulated necroptosis and mitochondrial dynamics. In summary, our study indicates that HNRNP A1 promotes the development of HCC, which suggests a potential therapeutic target for HCC.
Identification of Human Global, Tissue and Within-Tissue Cell-Specific Stably Expressed Genes at Single-Cell Resolution
Authors: Qiu Lingyu , Liang Chen , Zheng Yidong , Kang Huayu , Chen Aiyue , Chen Chunlin , Wang Xinlong , Yang Jielin , Fang Qiongfang , Hui Xinjie , Hu Yueming , Chen Zewei , Sha Ou , Zhu Wei-Guo , Wang Yejun
Published: September 2022

Stably Expressed Genes (SEGs) are a set of genes with invariant expression. Identification of SEGs, especially among both healthy and diseased tissues, is of clinical relevance to enable more accurate data integration, gene expression comparison and biomarker detection. However, it remains unclear how many global SEGs there are, whether there are development-, tissue- or cell-specific SEGs, and whether diseases can influence their expression. In this research, we systematically investigate human SEGs at single-cell level and observe their development-, tissue- and cell-specificity, and expression stability under various diseased states. A hierarchical strategy is proposed to identify a list of 408 spatial-temporal SEGs. Development-specific SEGs are also identified, with adult tissue-specific SEGs enriched with the function of immune processes and fetal tissue-specific SEGs enriched in RNA splicing activities. Cells of the same type within different tissues tend to show similar SEG composition profiles. Diseases or stresses do not show influence on the expression stableness of SEGs in various tissues. In addition to serving as markers and internal references for data normalization and integration, we examine another possible application of SEGs, i.e., being applied for cell decomposition. The deconvolution model could accurately predict the fractions of major immune cells in multiple independent testing datasets of peripheral blood samples. The study provides a reliable list of human SEGs at the single-cell level, facilitates the understanding on the property of SEGs, and extends their possible applications.
Significance of Hypermethylation of Tumor-Suppressor Genes PTGER4 and ZNF43 at CpG Sites in the Prognosis of Colorectal Cancer
Authors: Chen Chao-Yang , Wu Jia-Jheng , Lin Yu-Jyun , Hsu Chih-Hsiung , Hu Je-Ming , Chang Pi-Kai , Sun Chien-An , Yang Tsan , Su Jing-Quan , Chou Yu-Ching
Published: September 2022

The status of DNA methylation in primary tumor tissue and adjacent tumor-free tissue is associated with the occurrence of aggressive colorectal cancer (CRC) and can aid personalized cancer treatments at early stages. Tumor tissue and matched adjacent nontumorous tissue were extracted from 208 patients with CRC, and the correlation between the methylation levels of PTGER4 and ZNF43 at certain CpG loci and the prognostic factors of CRC was determined using the MassARRAY System testing platform. The Wilcoxon signed-rank test, a Chi-square test, and McNemar’s test were used for group comparisons, and Kaplan–Meier curves and a log-rank test were used for prediction. The hypermethylation of PTGER4 at the CpG_4, CpG_5, CpG_15, and CpG_17 tumor tissue sites was strongly correlated with shorter recurrence-free survival (RFS), progression-free survival (PFS), and overall survival (OS) [hazard ratio (HR) = 3.26, 95% confidence interval (CI) = 1.38–7.73 for RFS, HR = 2.35 and 95% CI = 1.17–4.71 for PFS, HR = 4.32 and 95% CI = 1.8–10.5 for OS]. By contrast, RFS and PFS were significantly longer in the case of increased methylation of ZNF43 at the CpG_5 site of normal tissue [HR = 2.33, 95% CI = 1.07–5.08 for RFS, HR = 2.42 and 95% CI = 1.19–4.91 for PFS]. Aberrant methylation at specific CpG sites indicates tissue with aggressive behavior. Therefore, the differential methylation of PTGER4 and ZNF43 at specific loci can be employed for the prognosis of patients with CRC.
SIRT1/FOXO Signaling Pathway in Breast Cancer Progression and Metastasis
Authors: Dilmac Sayra , Kuscu Nilay , Caner Ayse , Yildirim Sendegul , Yoldas Burcak , Farooqi Ammad Ahmad , Tanriover Gamze
Published: September 2022

Breast cancer is the second most common cancer in women. The roles of the SIRT and FoxO proteins in tumor progression are known, but their roles in metastasis have not yet been clearly elucidated. In our study, we investigated the roles of SIRT and FoxO proteins their downstream pathways, proteins p21 and p53, in tumor progression and metastasis. We evaluated these proteins in vitro using metastatic 4TLM and 67NR cell lines, as well as their expression levels in tumor-bearing mice. In addition, the regulatory role of SIRT and FoxO proteins in different transduction cascades was examined by IPA core analysis, and clinicopathological evidence was investigated in the TCGA database. In primary tumors, the expression levels of SIRT1, p21, p53, E2F1 and FoxO proteins were higher in 67NR groups. In metastatic tissues, the expression levels of SIRT1, E2F1 and FoxO proteins were found to be enhanced, whereas the levels of p53 and p21 expression were noted to be reduced. IPA analysis also provided empirical evidence of the mechanistic involvement of SIRT and FoxO proteins in tumor progression and metastasis. In conclusion, SIRT1 was found to co-operate with FoxO proteins and to play a critical role in metastasis. Additional research is required to determine why overexpression of SIRT1 in metastatic tissues has oncogenic effects.
Exopolysaccharides of Bacillus amyloliquefaciens Amy-1 Mitigate Inflammation by Inhibiting ERK1/2 and NF-κB Pathways and Activating p38/Nrf2 Pathway
Authors: Sung Wei-Wen , Lin Yun-Yu , Huang Shen-Da , Cheng Hsueh-Ling
Published: September 2022

Bacillus amyloliquefaciens is a probiotic for animals. Evidence suggests that diets supplemented with B. amyloliquefaciens can reduce inflammation; however, the underlying mechanism is unclear and requires further exploration. The exopolysaccharides of B. amyloliquefaciens amy-1 displayed hypoglycemic activity previously, suggesting that they are bioactive molecules. In addition, they counteracted the effect of lipopolysaccharide (LPS) on inducing cellular insulin resistance in exploratory tests. Therefore, this study aimed to explore the anti-inflammatory effect and molecular mechanisms of the exopolysaccharide preparation of amy-1 (EPS). Consequently, EPS reduced the expression of proinflammatory factors, the phagocytic activity and oxidative stress of LPS-stimulated THP-1 cells. In animal tests, EPS effectively ameliorated ear inflammation of mice. These data suggested that EPS possess anti-inflammatory activity. A mechanism study revealed that EPS inhibited the nuclear factor-κB pathway, activated the mitogen-activated protein kinase (MAPK) p38, and prohibited the extracellular signal-regulated kinase 1/2, but had no effect on the c-Jun-N-terminal kinase 2 (JNK). EPS also activated the anti-oxidative nuclear factor erythroid 2–related factor 2 (Nrf2) pathway. Evidence suggested that p38, but not JNK, was involved in activating the Nrf2 pathway. Together, these mechanisms reduced the severity of inflammation. These findings support the proposal that exopolysaccharides may play important roles in the anti-inflammatory functions of probiotics.
One Host-Multiple Applications: Zebrafish (Danio rerio) as Promising Model for Studying Human Cancers and Pathogenic Diseases
Authors: Dudziak Karolina , Nowak Michał , Sozoniuk Magdalena
Published: September 2022

In recent years, zebrafish (ZF) has been increasingly applied as a model in human disease studies, with a particular focus on cancer. A number of advantages make it an attractive alternative for mice widely used so far. Due to the many advantages of zebrafish, modifications can be based on different mechanisms and the induction of human disease can take different forms depending on the research goal. Genetic manipulation, tumor transplantation, or injection of the pathogen are only a few examples of using ZF as a model. Most of the studies are conducted in order to understand the disease mechanism, monitor disease progression, test new or alternative therapies, and select the best treatment. The transplantation of cancer cells derived from patients enables the development of personalized medicine. To better mimic a patient’s body environment, immune-deficient models (SCID) have been developed. A lower immune response is mostly generated by genetic manipulation but also by irradiation or dexamethasone treatment. For many studies, using SCID provides a better chance to avoid cancer cell rejection. In this review, we describe the main directions of using ZF in research, explain why and how zebrafish can be used as a model, what kind of limitations will be met and how to overcome them. We collected recent achievements in this field, indicating promising perspectives for the future.
Construction of a High-Density Recombination Bin-Based Genetic Map Facilitates High-Resolution Mapping of a Major QTL Underlying Anthocyanin Pigmentation in Eggplant
Authors: Guan Wenxiang , Ke Changjiao , Tang Weiqi , Jiang Jialong , Xia Jing , Xie Xiaofang , Yang Mei , Duan Chenfeng , Wu Weiren , Zheng Yan
Published: September 2022

High-density genetic maps can significantly improve the resolution of QTL mapping. We constructed a high-density recombination bin-based genetic map of eggplant based on 200 F2 plants from an interspecific cross (Solanum melongena × S. incanum) using the whole genome resequencing strategy. The map was 2022.8 cM long, covering near 99% of the eggplant genome. The map contained 3776 bins, with 3644 (96.5%) being effective (position non-redundant) ones, giving a nominal average distance of 0.54 cM and an effective average distance of 0.56 cM between adjacent bins, respectively. Using this map and 172 F2:3 lines, a major QTL with pleiotropic effects on two anthocyanin pigmentation-related traits, leaf vein color (LVC) and fruit pericarp color (FPC), was steadily detected in a bin interval of 2.28 cM (or 1.68 Mb) on chromosome E10 in two cropping seasons, explaining ~65% and 55% of the phenotypic variation in LVC and FPC, respectively. Genome-wide association analysis in this population validated the QTL and demonstrated the correctness of mapping two bins of chromosome E02 onto E10. Bioinformatics analysis suggested that a WDR protein gene inside the bin interval with reliable effective variation between the two parents could be a possible candidate gene of the QTL.
Simultaneous Probing of Metabolism and Oxygenation of Tumors In Vivo Using FLIM of NAD(P)H and PLIM of a New Polymeric Ir(III) Oxygen Sensor
Authors: Parshina Yulia P. , Komarova Anastasia D. , Bochkarev Leonid N. , Kovylina Tatyana A. , Plekhanov Anton A. , Klapshina Larisa G. , Konev Aleksey N. , Mozherov Artem M. , Shchechkin Ilya D. , Sirotkina Marina A. , Shcheslavskiy Vladislav I. , Shirmanova Marina V.
Published: September 2022

Tumor cells are well adapted to grow in conditions of variable oxygen supply and hypoxia by switching between different metabolic pathways. However, the regulatory effect of oxygen on metabolism and its contribution to the metabolic heterogeneity of tumors have not been fully explored. In this study, we develop a methodology for the simultaneous analysis of cellular metabolic status, using the fluorescence lifetime imaging microscopy (FLIM) of metabolic cofactor NAD(P)H, and oxygen level, using the phosphorescence lifetime imaging (PLIM) of a new polymeric Ir(III)-based sensor (PIr3) in tumors in vivo. The sensor, derived from a polynorbornene and cyclometalated iridium(III) complex, exhibits the oxygen-dependent quenching of phosphorescence with a 40% longer lifetime in degassed compared to aerated solutions. In vitro, hypoxia resulted in a correlative increase in PIr3 phosphorescence lifetime and free (glycolytic) NAD(P)H fraction in cells. In vivo, mouse tumors demonstrated a high degree of cellular-level heterogeneity of both metabolic and oxygen states, and a lower dependence of metabolism on oxygen than cells in vitro. The small tumors were hypoxic, while the advanced tumors contained areas of normoxia and hypoxia, which was consistent with the pimonidazole assay and angiographic imaging. Dual FLIM/PLIM metabolic/oxygen imaging will be valuable in preclinical investigations into the effects of hypoxia on metabolic aspects of tumor progression and treatment response.
Identification of the WRKY Gene Family and Characterization of Stress-Responsive Genes in Taraxacum kok-saghyz Rodin
Authors: Cheng Yifeng , Luo Jinxue , Li Hao , Wei Feng , Zhang Yuqi , Jiang Haiyang , Peng Xiaojian
Published: September 2022

WRKY transcription factors present unusual research value because of their critical roles in plant physiological processes and stress responses. Taraxacum kok-saghyz Rodin (TKS) is a perennial herb of dandelion in the Asteraceae family. However, the research on TKS WRKY TFs is limited. In this study, 72 TKS WRKY TFs were identified and named. Further comparison of the core motifs and the structure of the WRKY motif was analyzed. These TFs were divided into three groups through phylogenetic analysis. Genes in the same group of TkWRKY usually exhibit a similar exon-intron structure and motif composition. In addition, virtually all the TKS WRKY genes contained several cis-elements related to stress response. Expression profiling of the TkWRKY genes was assessed using transcriptome data sets and Real-Time RT-PCR data in tissues during physiological development, under abiotic stress and hormonal treatments. For instance, the TkWRKY18, TkWRKY23, and TkWRKY38 genes were significantly upregulated during cold stress, whereas the TkWRKY21 gene was upregulated under heat-stress conditions. These results could provide a basis for further studies on the function of the TKS WRKY gene family and genetic amelioration of TKS germplasm.
Complete Atrioventricular Block Caused by Retrograde Transaortic Approach
Authors: Chen Songwen , Lu Xiaofeng , Zhang Qitong , Wei Yong , Zhou Genqing , Liu Shaowen
Published: September 2022

A 61-year-old female was referred for catheter ablation of symptomatic and frequent premature ventricular complexes presented with right bundle branch block and a prominent inferior frontal plane QRS axis. A retrograde transaortic approach was routinely performed. A sustained complete atrioventricular block was repeatedly encountered while the ablation catheter was attempting to cross the aortic valve with different curves and manipulations. The procedure was abandoned. The mechanical atrioventricular block could only have been caused by the retrograde transaortic approach. We should be cautious when performing a retrograde transaortic catheter manipulation in some patients.
Morphometric Assessment for Functional Evaluation of Coronary Stenosis with Optical Coherence Tomography and the Optical Flow Ratio in a Vessel with Single Stenosis
Authors: Huang Yuming , Lin Zehuo , Wu Quanmin , Chen Liansheng , Yang Junqing , Deng Huiliang , Liu Yuanhui , Xie Nianjin
Published: September 2022

Objectives: The study aimed to evaluate the diagnostic performance of optical coherence tomography (OCT) in identifying functionally significant coronary stenosis in a vessel with single stenosis. Background: The OCT-based morphofunctional computational method for deriving the optical flow ratio (OFR) has diagnostic value, as it can identify the functional severity of coronary stenosis, but the ability of the OFR to aid the OCT in determining coronary stenosis hemodynamics in single-stenosis lesion remains unclear. Methods: 74 vessels with single stenosis were studied in 69 patients; all cases were performed through OCT and quantitative flow ratio (QFR), and OCT images were used to perform OFR. Results: Among vessels with single stenosis, OFR showed a good correlation with QFR (r = 0.86; p < 0.001). Taking QFR as the standard, the vessel-level diagnosis accuracy, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of OFR were 90% (95% CI: 81 to 96), 94% (95% CI: 77 to 99), 88% (95% CI: 74 to 96), 85% (95% CI: 68 to 94) and 95% (95% CI: 82 to 99), respectively. Among vessels with OFR/QFR concordance, both the minimum lumen area (MLA) and minimum lumen diameter (MLD) showed excellent diagnostic efficiency (MLA: area under the curve (AUC) = 0.92, 95% CI: 0.85 to 0.98, p < 0.001; MLD: AUC = 0.93, 95% CI: 0.86 to 0.98, p < 0.001) in determining the functional significance of coronary stenosis in a single stenosis lesion, and the best cutoff values were 1.55 mm2 and 1.40 mm. Conclusions: OFR has a good correlation with QFR. OCT-measured MLA and MLD have excellent diagnostic efficiency in identifying the hemodynamic significance of coronary stenosis in a vessel with single stenosis.