bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-parametric flow cytometry staining procedure for analyzing tumor-infiltrating immune cells following oncolytic herpes simplex virus immunotherapy in intracranial glioblastoma
Authors: K. Bommareddy Praveen , B. Lowe Devin , L. Kaufman Howard , D. Rabkin Samuel , Saha Dipongkor
Published: June 2019

Multi-color flow cytometry is a standard laboratory protocol, which is regularly used to analyze tumor-infiltrating immune cell subsets. Oncolytic herpes simplex virus has shown promise in treating various types of cancers, including deadly glioblastoma. Intracranial/intratumoral treatment with oncolytic herpes simplex virus expressing interleukin 12, i.e., immunovirotherapy results in induction of anti-tumor immune responses and tumor infiltration of a variety of immune cells. Multi-color flow cytometry is employed to characterize immune cells in the tumor microenvironment. Here, we describe a step-by-step 11-color flow cytometry protocol to stain tumor-infiltrating immune cells in glioblastoma following oncolytic herpes virotherapy. We also describe a method to identify HSV-1 glycoprotein-B-specific CD8+ T cells using fluorochrome-conjugated major histocompatibility complex multimers. The multimers carry major histocompatibility peptide complexes, which have the ability to interact and bind to T cell receptors present on the surface of T cells; allowing identification of T cells (e.g., CD8+) reactive to a desired antigen. This multimer staining can be used in conjunction with the multi-parametric flow cytometry staining. Brain tumor quadrants are harvested, minced, enzymatically digested, immune cells are isolated by positive selection, single cells are counted and blocked for Fc receptors, cells are incubated with dye and/or color-conjugated antibodies, and flow cytrometry is performed using a BD LSRII flow cytometer. The protocol described herein is also applicable to stain immune cells in other mouse and human tumors or in any desired tissues.
Primary cell-based phenotypic assays to pharmacologically and genetically study fibrotic diseases in vitro
Authors: Weigle Sabine , Martin Eva , Voegtle Andrea , Wahl Benjamin , Schuler Michael
Published: June 2019

Ongoing tissue repair and formation and deposition of collagen-rich extracellular matrix in tissues and organs finally lead to fibrotic lesions and destruction of normal tissue/organ architecture and function. In the lung, scarring is observed in asthma, chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis to various degrees. At the cellular level immune cells, fibroblasts and epithelial cells are all involved in fibrotic processes. Mechanistically, fibroblast to myofibroblast transformation and epithelial to mesenchymal transition are major drivers of fibrosis. Amongst others, both processes are controlled by transforming growth factor beta-1 (TGFβ-1), a growth factor upregulated in idiopathic pulmonary fibrosis lungs. Phenotypic assays with primary human cells and complex disease-relevant readouts becomeincreasingly important in modern drug discovery processes. We describe high-content screening based phenotypic assays with primary normal human lung fibroblasts and primary human airway epithelial cells. For both cell types, TGFβ-1 stimulation is used to induce fibrotic phenotypes in vitro, with alpha smooth muscle actin and collagen-I as readouts for FMT and E-cadherin as a readout for EMT. For each assay, a detailed image analysis protocols is described. Treatment of both cell types with TGFβ-1 and a transforming growth factor beta receptor inhibitor verifies the suitability of the assays for pharmacological interventions. In addition, the assays are compatible for siRNA and Cas9-ribonucleoprotein transfections, and thus are useful for genetic target identification/validation by modulating gene expression.
Hybrid project management for sociotechnical digital transformation context
Authors: Monteiro Cavalieri Barbosa Adriane , Pego Saisse Manoel Carlos
Published: June 2019

Goal: The path to implement Digital Transformation (DT) in modern industries is far from clear, particularly in Brazil. From the point of view of operations management, authors developed a Conceptual Framework (CF) to support DT implementation in Discrete Manufacturing Industries (DMI). The CF applies knowledge management, hybrid project management (HPM) and Sociotechnical values to implement DT, using New Product Development (NPD) as a ground. The work aims to depict the HPM approach of this CF.

Design / Methodology / Approach: this is a qualitative research based on a systematic literature review carried out over a period of five years. It was performed in the Web of Science database, applying PRISMA flowchart, to evaluate which project management models, addressed in the literature, are more suitable for DT implementation projects in light of sociotechnical and innovation-shared-values approach.

Results: A Hybrid “Stage-Gate – Agile - Design Thinking” project management model to support DT implementation in DMI. This is a preliminary proposal that can be explored in future empirical research.

Limitations of the investigation: Some quality papers may have been left out due to the research methodology.

Practical implications: The CF can be applied by professionals to support Digital Transformation while incorporating sociotechnical values and reducing the excessive technological bias in NPD projects.

Originality/Value: The authors propose a new Digital Transformation (DT) approach, named Sociotechnical Digital Transformation (SDT) and an HPM to support DT in light of a Sociotechnical approach.
Using multicriteria analysis and fuzzy logic for project portfolio management
Authors: Agápito Andrezza De Oliveira , Vianna Marcilene De Fátima Dianin , Moratori Patrick B. , Vianna Dalessandro Soares , Meza Edwin Benito Mitacc , Matias Italo De Oliveira
Published: June 2019

Goal: to propose a hybrid method, combining AHP and Fuzzy, for project portfolio management.

Originality/value: this paper meets the positive characteristics of both methods, adequately weighing the criteria and contemplating process subjectivity. Limitations that exist in both methods, such as the maximum number of alternatives and the difficulty of inserting new alternatives at the end of the process, are overcome.

Design/methodology/approach: the AHP is applied for determining the criteria weights and fuzzy is used to compare the alternatives for each criterion.

Results: the results show that the proposed hybrid method allows the ranking of many alternatives and provides higher reliability for decision makers. It must be noted that the system is fed by performance indicators, which minimize the subjectivity in decision-making—an important characteristic in the technology management. Moreover, the results provide the possibility of changing the number of projects at any time without influencing the outcome.

Practical implications: the proposed hybrid method can be used in different problems that have many alternatives or subjectivity.
Understanding Industry 4.0: Definitions and insights from a cognitive map analysis
Authors: Benitez Guilherme Brittes , Ferreira Lima Mateus José do Rêgo , Lerman Laura Visintainer , Frank Alejandro Germán
Published: June 2019

Goal: The purpose of this paper is to present an analysis of Industry 4.0 concepts and technologies through the lens of practitioners and scholars with considerable expertise in this field.

Design / Methodology / Approach: A qualitative research was conducted based on seven semi-structured interviews guided by an open questionnaire, which was developed with the main goal of understanding the concept of Industry 4.0 and the technologies that compose this industrial phenomenon.

Results: Based on this methodology, a cognitive map is presented as the result and final product of this study. This cognitive map is composed of five different clusters, each one represented by different colors, which relate to each of the questions in the questionnaire. Our analysis provides a better understanding of (i) the main concepts of Industry 4.0; (ii) the implementation stages for companies; (iii) the main enabling technologies; (iv) the concept of M2M (machine-to-machine); and (v) the scenario in Brazil. Each one of the clusters enables a discussion by bringing what is in the literature on the topic.

Limitations of the investigation: The main limitations of the article are found in the subjectivity of the results and also in the scarcity in the literature related to some topics covered in the clusters.

Practical implications: This research can be potentially useful for practitioners, since it sheds light on the Industry 4.0 concept and how technologies are used to integrate processes.

Originality / Value: The clusters answers of the cognitive map allowed comparisons with the literature and a discussion about Industry 4.0, making it possible to direct and check gaps in the research.
Innovation capability maturity in non-R&D performers: a reference model
Authors: Baptista Narcizo Ramon , Gabbay Canen Alberto , Tammela Iara , Cardoso Rodolfo , Eleutério Delesposte Jamile
Published: June 2019

Highlights: The prevalence of Research and Development (R&D) as an inducing factor for innovation has been questioned, and studies show that low-tech companies are able to innovate. Innovation capability is essential to increase productivity and improve competitiveness. However, when formal R&D is considered as the main parameter in its evaluation, it tends to underestimate it. The extent and heterogeneity of studies on innovation in non-R&D practitioners is a relevant problem and, despite theoretical and empirical advances, it is not satisfactorily resolved yet.

Goal: This article presents a reference model for innovation capability maturity in low-technology, non-R&D performing companies, oriented to guide decision makers and the institutions supporting these organizations.

Design / Methodology / Approach: The method used to construct the model is based on the proposition of Ahlemann and Gastl (2007) for the construction of reference models based on empirical evidence.

Results: The model is based on ten organizational dimensions and their effects on the organization's performance. Five maturity levels are defined, synthesizing the fundamental characteristics of innovation capability in non-R&D practitioners or low technology companies.

Limitations of the investigation: Although the model has been tested and validated, more applications and tests may still be required.

Practical implications: The model is an important tool for decision-making, at the management level, in low-tech companies that aspire to broaden their innovation capability.

Originality / Value: The model has significant potential academic repercussions, in terms of the expansion of knowledge, as well as for the industry, since it can contribute to the increase of the competitive performance of companies.
Design principles for research & development performance measurement systems: a systematic literature review
Authors: Oliveira Andre Ribeiro de , Proença Adriano
Published: June 2019

Goal: This paper aims to set the foundation on which a performance measurement system for the R&D function will be developed, assuming it not only as an organizational division, but also as a comprehensive process in order to better evaluate its efficiency and effectiveness.

Design/Methodology/Approach: This paper adopts a Design Science Research strategy as its methodological basis. It focuses on bringing guidelines from the body of organizational theory of knowledge to design effective management solutions for an R&D performance measurement system.

Results: A set of well-defined Design Principles to guide performance measurement system designs for R&D management.

Limitations of the investigation: Even though the design principles have been systematically obtained from the literature review, there was no adequacy test of these principles in practical cases, which should be conducted in future research.

Practical Implications: Managers and leaders of R&D teams may use theory-grounded guidelines to design their performance systems and to adjust such systems to their specific needs.

Originality/ Value: The main practical contribution of the study findings is to provide a comprehensive set of guidelines to design a R&D Performance Measurement System, rather than proposing another new complete theoretical framework to add to the literature.
Educação Especial como política pública: Um projeto do regime militar?
Authors: Kassar Monica de Carvalho Magalhães , Rebelo Andressa dos Santos , Jannuzzi Gilberta Sampaio de Martino
Published: June 2019

Este artigo aborda a formulação da Educação Especial como política pública nacional de educação. No conjunto do material pesquisado, encontram-se entrevista com a primeira Diretora Geral do Centro Nacional de Educação Especial, documentos internacionais, relatórios técnicos, legislação e estudos produzidos por solicitação do Ministério da Educação. Tais documentos são analisados considerando o movimento político da época, de modo a identificar argumentos e suas características filosóficas e ideológicas. O recorte histórico vai do final da década de 1950 a meados da década de 1980. São discutidas as particularidades da política constituída nesse momento e o modo como pessoas com deficiência passam a ser consideradas como alunos.
A formação de professores-pesquisadores em Educação Especial durante a ditadura no Brasil
Authors: Souza Flávia Faissal de , Pletsch Márcia Denise , Batista Getsename de Freitas
Published: June 2019

A institucionalização da Educação Especial ocorreu durante o regime da ditadura, com a criação do Centro Nacional de Educação Especial (Cenesp), mediante convênios internacionais para a formação de professores-pesquisadores na Educação Especial. O objetivo deste artigo é analisar a influência das diretrizes internacionais da ONU e dos Acordos MEC/Usaid na estruturação das propostas do Cenesp para formação de professores-pesquisadores da área. Para tal, realizamos uma pesquisa documental e uma entrevista semiestruturada com a primeira diretora-geral do Cenesp. Ao discutir o contexto internacional e nacional da época, nossa análise problematiza o lugar da pesquisa e da formação de professores para a Educação Especial.
Against the parallelism between the NP and the clause: Evidence from idioms
Authors: Cecchetto Carlo , Donati Caterina
Published: June 2019

We argue that the counterpart of Marantz’s generalization does not hold in the nominal domain, because there are idioms in which the determiner and the noun receive an idiomatic meaning while the PP that modifies the noun is not part of the idiom (we call these “PP-less idioms”). We show that PP-less idioms are fully expected if the hypothesis of parallelism between nominal structure and clausal structure is dropped and it is assumed that the first step of the derivation in the nominal domain involves merge of D and N.

As for the mirror image of PP-less idioms, “PP-containing idioms”, namely DPs where N and the PP that follows the noun receive an idiomatic reading while D does not, we suggest that they are not generated by syntax but are rather the output of the morphological component.
A study on numerical integration methods for rendering atmospheric scattering phenomenon
Authors: Gałaj Tomasz , Wojciechowski Adam
Published: June 2019

A qualitative comparison of three, popular and most widely known numerical integration methods in terms of atmospheric single scattering calculations is presented. A comparison of Midpoint, Trapezoidal and Simpson’s Rules taking into account quality of a clear sky generated images is performed. Methods that compute the atmospheric scattering integrals use Trapezoidal Rule. Authors try to determine which numerical integration method is the best for determining the colors of the sky and check if Trapezoidal Rule is in fact the best choice. The research does not only conduct experiments with Bruneton’s framework but also checks which of the selected numerical integration methods is the most appropriate and gives the lowest error in terms of atmospheric scattering phenomenon.
Prediction of Seismic Performance Levels of Corroded Reinforced Concrete Columns as a Function of Crack Width
Authors: Yalciner Hakan , Kumbasaroglu Atila , Karimi Aqludin
Published: June 2019

An experimental study was conducted for the prediction of the seismic performance levels of corroded reinforced concrete (RC) columns as a function of the initial corrosion crack widths. A full-scale accelerated-corrosion pool was used to corrode 25 full-scale RC columns. The initial crack widths at different levels of corrosion were measured for three different concrete strength levels: 9, 27, and 37 MPa. The seismic performance levels of corroded RC columns under combined cyclic lateral-displacement excursions at two different axial-load ratios (0.20 and 0.40) were measured. The corrosion levels obtained for the initially measured corrosion crack widths were used for establishing a correlation with the lateral-displacement capacities of the RC columns according to an energy-based method. Three empirical models were developed. The first was for predicting the cross-sectional area reduction of reinforcement bars according to the initial corrosion crack widths. The second model was for predicting the percentage of energy capacity of RC columns as a function of the drift ratio and corrosion levels. The third model was for predicting the seismic performance levels of RC columns as a function of the initial corrosion crack widths and was used for an in situ structural assessment. The experimental test results showed that above a 2% drift ratio, all uncorroded RC columns (regardless of the concrete strength levels) exhibited a sudden reduction in energy capacity, and their total energy capacity was consumed. In the case of corrosion, the energy capacity of concrete with high strength levels was prematurely exhausted above a 1.4% drift ratio because of the effects of the corrosion products under the high strength levels of the concrete.
The DomesticBCO2Allele Buffers Low-Carotenoid Diets in Chickens: Possible Fitness Increase Through Species Hybridization
Authors: Fallahshahroudi Amir , Sorato Enrico , Altimiras Jordi , Jensen Per
Published: June 2019

Domestic animals are adapted to conditions vastly different from those of their wild ancestors, and this is particularly true for their diets. The most numerous of all domestic species, the chicken, originated from the Red Junglefowl (RJF), a native of subtropical forests in Southeast Asia. Surprisingly however, in domestic chicken breeds, a common haplotype of the β-carotene oxygenase 2 (BCO2) gene, which is involved in carotenoid metabolism, is introgressed from a related species, the Gray Junglefowl, and has been under strong selective pressure during domestication. This suggests that a hybridization event may have conferred a fitness advantage on chickens carrying the derived allele. To investigate the possible biological function of the introgressed BCO2 allele in chicken, we introgressed the ancestral BCO2 allele into domestic White Leghorn chickens. We measured gene expression as well as carotenoid accumulation in skin and eggs of chickens carrying either the ancestral or the derived BCO2 allele. The derived haplotype was associated with down-regulation of BCO2 in skin, muscle, and adipose tissue, but not in liver or duodenum, indicating that carotenoid accumulation occurred in the tissues with reduced gene expression. Most importantly, we found that hens with the derived BCO2 genotype were capable of allocating stored carotenoids to their eggs, suggesting a functional benefit through buffering any shortage in the diet during egg production. Nevertheless, it is of interest that loss of function mutations in BCO2 gene are prevalent in other domesticates including cows, rabbits, and sheep, and, given the importance of carotenoids in development, reproduction, and immunity, it is possible that derived BCO2 alleles may provide a general mechanism in multiple domestic species to deal with higher demand for carotenoids in an environment with carotenoid shortage in the diet.
“Just” Stories or “Just Stories”?: Mixed Media Storytelling as a Prism for Environmental Justice and Decolonial Futures
Authors: Wiebe Sarah Marie
Published: June 2019

Our lives and the lives of those we study are full of stories. Stories are never mere stories. Qualitative researchers who document, hear, and listen to participant lived-experiences encounter and witness the intimate spaces of people’s everyday lives. Researchers thus find themselves in the position of translator between diverse communities: those affected by policies, the academy and public officials. For academic-activists committed to listening to situated stories in order to improve public policy, several critical questions emerge: How do we do justice to these stories? What are the ethics of engagement involved in telling stories about those who share their knowledges and lived-experiences with us? Can storytelling bridge positivist and post-positivist research methods? Do policymakers listen to stories? How? What can researchers learn from Indigenous storytelling methods to envision decolonial, sustainable futures? To respond to these critical questions, this paper draws from literature in community-engaged research, critical policy studies, interpretive research methods, Indigenous research methods, political ethnography, visual methods and social justice research to argue that stories arenever simply or just stories, but in fact have the potential to be radical tools of change for social and environmental justice. As will be discussed with reference to three mixed media storytelling projects that involved the co-creation of digital stories with Indigenous communities in Canada, stories can intervene on dominant narratives, create space for counternarratives and in doing so challenge the settler-colonial status quo in pursuit of decolonial futures.
Drawing Gender Equality: A Participatory Action Research Project with Educators in Northern Uganda
Authors: Jones Shelley
Published: June 2019

This paper reports upon an arts-based participatory action research project conducted with a cohort of 30 teachers in rural Northwest Uganda during a one-week professional development course. Multimodality (Kress & Jewitt, 2003; Kress & van Leeuwen, 2001) was employed as a “domain of inquiry” (Kress, 2011) for social semiotics (meaning-making within a social context) within which the participants both represented gender inequality as well as imagined gender equality. Multimodality recognizes the vast communicative potential of the human body and values multiple materials resources (such as images, sounds, and gestures) as “organized sets of semiotic resources for meaningmaking” (Jewitt, 2008, p. 246). Providing individuals with communicative modes other than just spoken and written language offers opportunities to include voices that are often not heard in formal contexts dominated by particular kinds of language, as well as opportunities to consider topics of inquiry from different perspectives and imagine alternative futures (Kendrick & Jones, 2008). Findings from this study show how a multimodal approach to communication, using drawing in addition to spoken and written language, established a democratic space of communication. The sharing and building of knowledge between the participants (educators in local contexts) and facilitator (university instructor/researcher) reflected a foundational tenet of engaged scholarship which requires “…not only communication to  public audiences, but also collaboration with communities in the production of knowledge” (Barker, 2004, p. 126).
Reflecting on my Assumptions and the Realities of Arts-Based Participatory Research in an Integrated Dance Community
Authors: Acton Kelsie
Published: June 2019

The arts-based research paradigm prioritizes creativity, relationships and the potential of transformative change (Conrad & Beck, 2016). Arts-based research may be useful in disability communities where people may prefer to communicate artistically or through movement, rather than through spoken word (Eales & Peers, 2016). Participatory action research (PAR) involves researchers working with communities to create research critical of dominant power relations and responsive to the needs of communities (McIntyre, 2008). Both arts-based research and PAR value an axiological approach that is responsive to the community’s needs over a dogmatic procedure, meaning that researchers must be reflexive and responsive to the often unexpected realities of the field. Over four months in 2017, eight dancers/researchers from CRIPSiE (Collaborative Radically Integrated Performers Society in Edmonton), an integrated dance company, came together to investigate how integrated dancers practice elements of timing in rehearsal, through an arts-based, participatory process. In this paper I examine the gap between my assumptions of how research should be conducted and the reality of the field, specifically: the tension between university research ethics and the ethics of the CRIPSiE community, the differences between the value of the rehearsal process and the performance as sites of data collection, and the assumptions I had made about the necessity of a singular research question.
Reading Experience as Communitist Practice: Indigenous Literatures and Community Service-Learning
Authors: McDougall JD , Styvendale Nancy Van
Published: June 2019

Our paper analyzes a community service-learning class on Indigenous literatures from the perspectives of graduate student and instructor. Enacting Jace Weaver’s theory of communitism (a portmanteau of “community” and “activism”), the class asks students to read Indigenous texts through the lens of their experiences at communitybased organizations in Saskatoon, Saskatchewan, and to consider how these readings shape their interactions with and responsibilities to Indigenous communities. First, the instructor discusses the complexities of community service-learning as an engaged approach to literary study in a settler colonial context. Informed by Tomson Highway’s novel Kiss of the Fur Queen, the second author then analyzes their1 contributions to the social justice club at Oskāyak High School, highlighting Oskāyak’s unique academic culture, where music and Indigenous language learning are incorporated into the fabric of everyday life. Ultimately, we argue that a communitist approach to Indigenous literary scholarship creates or furthers relationships with/in and responsibility to Indigenous communities, while encouraging an integrative approach to literary study through critical embodiment.
Woolly Stories: An Art-Based Narrative Approach to Place Attachment
Authors: Stiwich Kendra D. , McCunn Lindsay, J. , Dayal Chantey
Published: June 2019

When people join an institution, no assurance of positive social connection exists. The mechanisms of psychological attachment to institutions are not well understood. However, place attachment is a predictor of individual well-being and, when correlated with life satisfaction and neighborhood ties, can enhance civic engagement and social trust. Research suggests that narratives can be a symbolic mechanism of place attachment. Thus, to increase place attachment in the parent population at a small elementary school,  various art-based narrative activities were carried out as part of the OurSchoolOurStories project. Creating a storied blanket was one activity. Seven women needle-felted nine squares with the theme of representing some aspect of what the school meant to them. In a circle, they shared many stories including where they came from, how they came to be at the school, and their experiences at the school. Through these artistic narratives, participants were able to share much about their place identities, which allowed for social connection, and a sense of integration within the group.
Grassroots Leadership and the Arts for Social Change by S.J. Erenrich and J.F. Wergin (Eds.).
Authors: Daether Vanessa
Published: June 2019

Erenrich and Wergin’s edited book, Grassroots Leadership and the Arts for Social Change, explores the tandem roles of the arts in facilitating grassroots movements and the leaders who have inspired them. Founded in Erenrich’s expertise as a scholar and practitioner of leadership, social change, and the arts, and in Wergin’s distinguished academic background as a professor and author in the fields of higher education and leadership, this book presents sixteen unique narratives clearly articulating the importance of the arts as a vehicle for creating change from the ground-up.
CORRECTION: A type 2 diabetes mellitus patient was successfully treated by autologous bone marrow-derived stem cell transplantation: A case report
Authors: Le Phuong Thi-Bich , Doan Nguyen Phu-Van , Tien Phan Van , Hoang Dang Ngo Chau , Phan Ngoc Kim , Pham Phuc Van
Published: June 2019

In the previous publication with title ”A type 2 diabetes mellitus patient was successfully treated by autologous bone marrow-derived stem cell transplantation: A case report”1, page 2968, in the Table 1, the unit for fasting blood glucose and blood glucose 2 hours after meals is mg/dL; however, the corrected unit for fasting blood glucose and blood glucose 2 hours after meal is mmol/L.
Comparison of the efficacy of using paclitaxel-eluting balloon and plain balloon angioplasty for arteriovenous fistula in hemodialysis patients
Authors: Rai Alireza , Sobhiyeh Mohammadreza
Published: June 2019

Introduction: Arteriovanous (AV) access failure is one of the main problems in patients with end stage renal disease (ESRD), who receive hemodialysis. Balloon angioplasty is a favorable method for managing vascular access failure. The purpose of this study was to compare the six-month efficacy of paclitaxel-eluting balloon and plain balloon angioplasty in failed AV access cases among hemodialysis patients.

Methods: In this quasi-experimental study (http://en.irct.ir/trial/35333), 50 hemodialysis patients with failure of AV access (stenosis > 50%), who were candidates for angioplasty, were included. They were divided to receive either paclitaxel-eluting balloon (25 patients) or plain balloon (25 patients) angioplasty. Patients were followed up for six months with color Doppler ultrasonography and clinical examination for the hemodynamic success rate of angioplasty.

Results: After six months, 19 patients (76%) in paclitaxel-eluting balloon angioplasty group achieved hemodynamic success, which was significantly higher than plain balloon angioplasty group (13 patients, 52%) (P = 0.012). Age, gender, diabetes mellitus, hypertension, and location of AVF (snuff box, forearm, and antecubital fossa) did not associate with hemodynamic success rate in any group.

Conclusion: The use of angioplasty with paclitaxel-eluting balloon was superior to plain balloon angioplasty for failed AV access cases in hemodialysis patients. It is recommended to use paclitaxeleluting balloon angioplasty in patients with failure of AV access and requirement for balloon angioplasty.
Krónikus légzőszervi betegek tápláltsági állapotának vizsgálata – klinikai megfigyelések
Authors: Fekete Mónika , Pongor Vince , Fehér Ágnes , Veresné Bálint Márta , Varga János Tamás , Horváth Ildikó
Published: June 2019

Abstract:

Introduction: The increased metabolism of nutrients and the low energy intake may lead to malnutrition among chronic obstructive pulmonary disease (COPD) patients. Aim: The goal of our study was to examine the nutritional status of our population aged over 40, and its relationship with the severity of the disease. Method: We conducted a retrospective study at the National Korányi Institute of Pulmonology in 2017. Pulmonary function and anthropometric data were obtained from the electronic health record system. Inclusion criteria were age over 40 and the diagnosis of COPD. Severity of disease was assessed by forced expiration volume and categorized according to GOLD stages. We used SPSS Statistics V22.0 for data analysis. Results: The mean age of participants was 66; 49.3% were men, 50.7% were women. Average BMI was 27.14 kg/m², with values comprising cachexia and severe obesity. According to the FEV1%pred results of the 3236 patients, 30% fell in the GOLD I, 40% in the GOLD II, 23% in the GOLD III, and 7% in the GOLD IV categories. Pearson coefficient found positive correlation between FEV1 and nutritional status (H = 0.2297, r = 0.1401), specifically between severity of cachexia and severity of disease. The analysis of variance showed significant correlation between severity of disease and nutritional status; patients with higher BMI had better pulmonary function. Conclusion: Malnutrition had an adverse effect on pulmonary functions and performance of respiratory muscles, whereas higher BMI had a positive effect on FEV1. Our results suggest that BMI could be used as a lung function prognostic indicator for COPD patients. Orv Hetil. 2019; 160(23): 908–913.
Az onkológiában alkalmazott immuncheckpointgátló terápiák autoimmun mellékhatásai: patogenezis, klinikum és terápia
Authors: Szekanecz Éva , Szekanecz Zoltán
Published: June 2019

Abstract:

Oncotherapy has been revolutionised by the introduction of immune-checkpoint inhibitors including CTLA4, PD1 and PDL1 inhibitors. Patients with malignant diseases may largely benefit from these therapies, which may result in long-term remission even in the most therapy-resistant tumour types. Differences in the mode of action of the various agents may result in varying side-effect profiles. In addition to organ-specific side-effects, overt autoimmune syndromes may also develop. Our current view of oncotherapy has changed as these mostly immune-mediated side-effects highly differ from those observed previously during the administration of traditional anti-tumour compounds. These side-effects should be carefully characterized and differentiated from infections or the progression of the underlying malignancy. Fortunately, several recent recommendations have become available on the management of immune-mediated adverse events due to checkpoint-inhibitor therapy. Orv Hetil. 2019; 160(23): 887–895.
A DADA drogprevenciós program hatékonyságvizsgálata hetedik évfolyamos általános iskolai tanulók körében
Authors: Danku Nóra , Fekete Rita , Fekete Sándor , Boncz Imre
Published: June 2019

Abstract:

Introduction: Drug-taking habit is not infrequent in the young population. There is a need of proven effective drug prevention programs. Aim: The aim of our study was to analyze the efficiency of the Hungarian Police’s DADA school-drug prevention program. Method: The research program was carried out by a standard questionnaire at Apáczai Csere János Primary School in Pécs and Kodolányi János Primary School in Pécsvárad. Results: After analyzing the participating students’ answers, the results showed that the rate of those, who “know everything” about drugs, increased from 27% to 37.3%, and of those, who did “not know anything”, decreased from 5.6% to 1.6% (p<0.001). Regarding self-efficiency, the DADA program did not show any significant change (p = 0.364). However, less than 60% of the students agreed the statements: “I have learned a lot from the trainings” and “I received answers to several questions that I had been interested in earlier.” Conclusion: We can conclude that the DADA program was successful. Regarding the chosen reactions and opinions about the program, we have to consider the sociocultural background of the participants. Orv Hetil. 2019; 160(23): 914–920.
SYNTHESIS AND ANTIBACTERIAL ACTIVITY OF COMPOSITE BASED ON CHITOSAN-GRAFTED-(N-BUTYL ACRYLATE) AND SILVER NANOPARTICLES
Authors: Ha La Thi Thai , Mai Chau Ngoc
Published: June 2019

It has been found for a long time that chitosan (CS) and silver nanoparticles (AgNPs) have outstanding antibacterial activities but there were some drawbacks restricting their wide utilization. In this research, CS modified is combined with AgNPs to expand applications and enhance the antibacterial activities. The colloid of CS and AgNPs (CS/Ag) was synthesized via chemical reduction while grafting copolymerization was carried out with monomer n-butyl acrylate (BA) and initiator tert-butyl hydroperoxide (TBHP) generating composite between CS-g-BA/Ag. The effects of parameters on synthesizing CS-g-BA/Ag composite were studied by determining the grafting percentage (G%) and grafting efficiency (E%).
STUDY ON CHEMICAL CONSTITUENT AND BIOACTIVITIES OF THE FRUITS OF DIPTEROCARPUS RETUSUS BLUME. DIPTEROCARPACEAE OF VIETNAM
Authors: Hung Ho Dac , Tien Doan Huy , Ngoan Nguyen Thi , Duong Ba Thi , Viet Do Quoc , Dien Pham Gia , Anh Bui Kim
Published: June 2019

The first study on chemical constituents and biological activities of fruits of Dipterocarpus retusus Blume (Dipterocarpaceae.) growing in Vietnam was reported. Column chromatography of the ethyl acetate extract led to the isolation of five compounds: eleutherol (1), 3, 5, 4' - trihydroxy - trans - stilbene (2), polydatin (3) and β-sitosterol (4), β-sitosterol-3-O-β-D-glucopyranoside (5). Their structures were elucidated based on the NMR spectroscopic methods and comparison with literature date. The extracts from fruits of D. retusus Blume by n-hexane, ethyl acetate and butanol, consecutively, were evaluated for antioxidative and cytotoxic activities.
INFLUENCE OF A POLYURETHANE DIACRYLATE AND HEXANEDIOL DIACRYLATE RATIO ON THE HOTOCROSSLINKING AND PROPERTIES OF UV-CURED COATINGS
Authors: Dao Phi Hung , Le Hien Xuan
Published: June 2019

Influence of a polyurethane dicarylate (UDA) and hexanediol diacrylate (HDDA) ratio on the photocrosslinking and properties of the systems based on UDA, HDDA and photoinitiator I.184 has been investigated. It was demonstrated that in the range of the UDA/HDDA ratio from 80/20 to 20/80, the fine, smooth films were obtained when the ratios were from 60/40 to 40/60 and the lower UDA/HDDA ratio, the faster conversion of acrylate groups. The acrylate groups in the investigated coatings were converted rapidly, leading to formation of tridimensional polymer networks and change of physico-mechanical properties of the coatings.
Risk of Osteoporosis and Fractures in Patients with Thyroid Cancer: A Case-Control Study in U.S. Veterans
Authors: Papaleontiou Maria , Banerjee Mousumi , Reyes-Gastelum David , Hawley Sarah T. , Haymart Megan R.
Published: June 2019

Background

Data on osteoporosis and fractures in patients with thyroid cancer, especially men, are conflicting. Our objective was to determine osteoporosis and fracture risk in U.S. veterans with thyroid cancer.

Materials and Methods

This is a case-control study using the Veterans Health Administration Corporate Data Warehouse (2004–2013). Patients with thyroid cancer (n = 10,370) and controls (n = 10,370) were matched by age, sex, weight, and steroid use. Generalized linear mixed-effects regression model was used to compare the two groups in terms of osteoporosis and fracture risk. Next, subgroup analysis of the patients with thyroid cancer using longitudinal thyroid-stimulating hormone (TSH) was performed to determine its effect on risk of osteoporosis and fractures. Other covariates included patient age, sex, median household income, comorbidities, and steroid and androgen use.

Results

Compared with controls, osteoporosis, but not fractures, was more frequent in patients with thyroid cancer (7.3% vs. 5.3%; odds ratio [OR], 1.33; 95% confidence interval [CI], 1.18–1.49) when controlling for median household income, Charlson/Deyo comorbidity score, and androgen use. Subgroup analysis of patients with thyroid cancer demonstrated that lower TSH (OR, 0.93; 95% CI, 0.90–0.97), female sex (OR, 4.24; 95% CI, 3.53–5.10), older age (e.g., ≥85 years: OR, 17.18; 95% CI, 11.12–26.54 compared with <50 years), and androgen use (OR, 1.63; 95% CI, 1.18–2.23) were associated with osteoporosis. Serum TSH was not associated with fractures (OR, 1.01; 95% CI, 0.96–1.07).

Conclusion

Osteoporosis, but not fractures, was more common in U.S. veterans with thyroid cancer than controls. Multiple factors may be contributory, with low TSH playing a small role.

Implications for Practice

Data on osteoporosis and fragility fractures in patients with thyroid cancer, especially in men, are limited and conflicting. Because of excellent survival rates, the number of thyroid cancer survivors is growing and more individuals may experience long-term effects from the cancer itself and its treatments, such as osteoporosis and fractures. The present study offers unique insight on the risk for osteoporosis and fractures in a largely male thyroid cancer cohort. Physicians who participate in the long-term care of patients with thyroid cancer should take into consideration a variety of factors in addition to TSH level when considering risk for osteoporosis.
Axial test of full-scale columns strengthened with jacket section
Authors: Hwang Yong-Ha , Yang Keun-Hyeok , Mun Ju-Hyun
Published: June 2019

This study examines the effect of different arrangements of transverse reinforcement in the jacket section on the axial performance of full-scale reinforced-concrete columns strengthened with a seismic section enlargement approach. For the jacket section with seismic details, overlapped channel-shape bars and prefabricated bar units were designed as the closed peripheral hoops, and V-ties or segmented cross-ties were used for the supplementary transverse reinforcement. For the compressive tests of full-scale strengthened columns with a 750 mm square section, a steel loading frame with a 40 000 kN capacity hydraulic jack was specially devised. Test results showed that the strengthened columns without supplementary V-ties in the jacket section possessed lower ductility capacity when compared with that of the existing non-seismic column. At the same level of transverse reinforcement index, the axial ductility ratio obtained from the present full-scale columns was typically lower than that compiled from the scaled-down column specimens with a square section of 200–300 mm. However, the strengthened column with prefabricated bar unit hoops including supplementary V-ties exhibited good ductility, which was comparable to the axial ductility ratios obtained in scaled-down columns, although a size effect potentially induced a more rapid decreasing rate at the descending branch of the stress–strain relationship of the concrete.
Energy supplements for leucaena
Authors: Harper Karen , Quigley Simon P. , Antari Risa , Dahlanuddin - , Panjaitan Tanda Sahat , Marsetyo - , Poppi Dennis P.
Published: June 2019

Keynote paper presented at the International Leucaena Conference, 1‒3 November 2018, Brisbane, Queensland, Australia.Leucaena can be fed as the sole diet to fattening cattle without nutritional problems and it will promote high liveweight gains. The high crude protein concentration in leucaena suggests that energy supplements, which are readily fermented in the rumen, could be used to capture the excess rumen degradable protein and provide more microbial protein and metabolizable energy to the animal, further increasing liveweight gain or milk production. This approach has been tested in grazing cattle and also in cut-and-carry systems in Australia and Indonesia. In both systems, production (liveweight gain or milk production) increased with the addition of supplements containing large amounts of fermentable meta- bolizable energy. The substitution of the basal diet (leucaena or leucaena mixed with grass or crop residues) by the supplement also means that more animals can be carried in the system for a set amount or area of leucaena. The same principles would apply to any tree legume-based system. Energy supplements can come in many forms, viz. fermentable starch (cereal grains and cassava), sugars (molasses), pectins (soybean hulls and pulps) and fibre (rice bran, cassava bagasse), but they have not been compared for their efficacy nor for their economic benefit, if any, in these systems.
Leucaena cultivars – current releases and future opportunities
Authors: Dalzell Scott A.
Published: June 2019

Plenary paper presented at the International Leucaena Conference, 1‒3 November 2018, Brisbane, Queensland, Australia.The Leucaena genus is made up of 24 different species (19 diploid and 5 tetraploid species). However, early use of the Leucaena genus in commercial agricultural systems was based entirely upon a very narrow germplasm base. A single genotype of Leucaena leucocephala ssp. leucocephala (‘common’ leucaena) was spread pantropically from its center of origin in Mexico over 400 years ago. Genetic improvement of Leucaena leucocephala began in the 1950s, when vigorous ‘giant’ leucaena (L. leucocephala ssp. glabrata) was identified in Australia and Hawaii. Cultivars such as Hawaiian Giant K8, Peru and El Salvador were selected and promoted for grazing in Australia and multipurpose agroforestry uses throughout the tropics. Plant breeding for improved forage production resulted in the release of cv. Cunningham in 1976 in Australia. These cultivars of ‘giant’ Leucaena leucocephala displayed broad environmental adaptability, with the exception of poor tolerance of cold temperatures (and frost) and acid soils. The outbreak of the psyllid insect pest (Heteropsylla cubana) from Cuba during the 1980s devastated both ‘common’ and ‘giant’ leucaena all around the world. This challenge resulted in renewed interest in lesser-known Leucaena spp. that exhibited tolerance to the pest and in interspecific hybridization as a means of developing new cultivars. Some ‘giant’ leucaena lines exhibited excellent agronomic traits and a degree of tolerance to the psyllid pest and this resulted in the release of new cultivars in Australia (cvv. Tarramba and Wondergraze) and Hawaii (cv. LxL). Since the 1990s, plant breeding programs have sought to develop cultivars with greater psyllid tolerance using interspecific hybridization. This has resulted in the release of cv. ‘KX2-Hawaii’ for timber and forage production, and a backcrossed forage cultivar cv. Redlands (Australia). Both cultivars are based upon interspecific hybridization between L. pallida and L. leucocephala ssp. glabrata. Cold-temperature and acid-soil tolerance have been pursued in South American breeding programs based upon L. diversifolia, without commercial success. The development of sterile Leucaena spp. cultivars is currently underway to nullify the environmental weed potential of all current commercial cultivars. Tolerance to cold temperatures (L. diversifolia, L. pallida, L. pulverulenta and L. trichandra), frost (L. greggii and L. retusa) and psyllids (L. collinsii) exists within the Leucaena genus and may be exploited in future hybridization programs. New genetic analyses and molecular plant breeding techniques have the potential to facilitate further gene transfer between Leucaena spp. for the development of the next generation of multipurpose cultivars.
Vegetative and micropropagation of leucaena
Authors: Idol Travis , Youkhana Adel , Santiago Renier Paul
Published: June 2019

Keynote paper presented at the International Leucaena Conference, 1‒3 November 2018, Brisbane, Queensland, Australia.To effectively utilize sterile hybrids of leucaena, efficient protocols for vegetative propagation are needed that meet different user requirements and capabilities. We developed and compared methods for propagating several sterile hybrids of leucaena and compared them with each other and with propagation via seeds for variety K636. Methods included air-layers, rooted cuttings, grafting and tissue culture (micropropagation). All methods required 14‒20 weeks from generation of new shoots on the stock plant to production of rooted plantlets ready to outplant as compared with 6‒8 weeks for seedlings of K636. Successful rooting was highest for air-layers and rooted cuttings. Grafting had lower success owing to a higher skill requirement for the propagator. Tissue culture showed promise, but use of field-grown material was limited by microbial contamination of propagation media. Rooted cuttings are the best option presently for operational-scale propagation, but the method requires a mist system or a carefully controlled non-mist environment. If an effective method can be developed, grafting of young shoots onto a seedling rootstock is an alternative that retains the advantages of a seedling tap root and requires fewer resources than rooted cuttings or tissue culture.