bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of Forged Images Using a Combination of Passive Methods Based on Neural Networks
Authors: Alencar Ancilon Leuch , Lopes Marcelo Dornbusch , Fernandes Anita Maria da Rocha , Anjos Julio Cesar Santos dos , De Paz Santana Juan Francisco , Leithardt Valderi Reis Quietinho
Published: March 2024

In the current era of social media, the proliferation of images sourced from unreliable origins underscores the pressing need for robust methods to detect forged content, particularly amidst the rapid evolution of image manipulation technologies. Existing literature delineates two primary approaches to image manipulation detection: active and passive. Active techniques intervene preemptively, embedding structures into images to facilitate subsequent authenticity verification, whereas passive methods analyze image content for traces of manipulation. This study presents a novel solution to image manipulation detection by leveraging a multi-stream neural network architecture. Our approach harnesses three convolutional neural networks (CNNs) operating on distinct data streams extracted from the original image. We have developed a solution based on two passive detection methodologies. The system utilizes two separate streams to extract specific data subsets, while a third stream processes the unaltered image. Each net independently processes its respective data stream, capturing diverse facets of the image. The outputs from these nets are then fused through concatenation to ascertain whether the image has undergone manipulation, yielding a comprehensive detection framework surpassing the efficacy of its constituent methods. Our work introduces a unique dataset derived from the fusion of four publicly available datasets, featuring organically manipulated images that closely resemble real-world scenarios. This dataset offers a more authentic representation than other state-of-the-art methods that use algorithmically generated datasets based on image patches. By encompassing genuine manipulation scenarios, our dataset enhances the model’s ability to generalize across varied manipulation techniques, thereby improving its performance in real-world settings. After training, the merged approach obtained an accuracy of 89.59% in the set of validation images, significantly higher than the model trained with only unaltered images, which obtained 78.64%, and the two other models trained using images with a feature selection method applied to enhance inconsistencies that obtained 68.02% for Error-Level Analysis images and 50.70% for the method using Discrete Wavelet Transform. Moreover, our proposed approach exhibits reduced accuracy variance compared to alternative models, underscoring its stability and robustness across diverse datasets. The approach outlined in this work needs to provide information about the specific location or type of tempering, which limits its practical applications.
Fly by Feel: Flow Event Detection via Bioinspired Wind-Hairs
Authors: Court Alecsandra , Bruecker Christoph
Published: March 2024

Bio-inspired flexible pillar-like wind-hairs show promise for the future of flying by feel by detecting critical flow events on an aerofoil during flight. To be able to characterise specific flow disturbances from the response of such sensors, quantitative PIV measurements of such flow-disturbance patterns were compared with sensor outputs under controlled conditions. Experiments were performed in a flow channel with an aerofoil equipped with a 2D array of such sensors when in uniform inflow conditions compared to when a well-defined gust was introduced upstream and was passing by. The gust was generated through the sudden deployment of a row of flaps on the suction side of a symmetric wing that was placed upstream of the aerofoil with the sensors. The resulting flow disturbance generated a starting vortex with two legs, which resembled a horseshoe-type vortex shed into the wake. Under the same tunnel conditions, PIV measurements were taken downstream of the gust generator to characterise the starting vortex, while further measurements were taken with the sensing pillars on the aerofoil in the same location. The disturbance pattern was compared to the pillar response to demonstrate the potential of flow-sensing pillars. It was found that the pillars could detect the arrival time and structural pattern of the flow disturbance, showing the characteristics of the induced flow field of the starting vortex when passing by. Therefore, such sensor arrays can detect the “footprint” of disturbances as temporal and spatial signatures, allowing us to distinguish those from others or noise.
Enzyme-Assisted Ultrasonic Extraction and Antioxidant Activities of Polysaccharides from Schizochytrium limacinum Meal
Authors: Zhang Nuohan , Chen Wenwei , Li Xinyu , Chen Xinmiao , Wang Yuchen , Huang Guangrong , Wang Jiaxian , Jia Zhenbao
Published: March 2024

Enzyme-assisted ultrasonic extraction (EAUE) was utilized and optimized for extracting polysaccharides from Schizochytrium limacinum meal (SLMPs) via the response surface methodology. The optimal EAUE conditions were determined as follows: enzyme concentration at 5.18%, ultrasonic temperature at 53 °C, ultrasonic duration of 40 min, ultrasonic power at 60 W, and a liquid-to-material ratio of 34 mL/g, achieving a polysaccharide extraction yield of 11.86 ± 0.61%. The purified polysaccharide component, SLMP1-1, isolated using DEAE Sepharose Fast Flow and Sephadex G-100 columns, exhibited potent antioxidant activity. SLMP1-1, with a molecular weight of 25.5 kDa, comprises glucose, mannose, arabinose, and galactose in a molar ratio of 16.39:14.75:1:693.03. 1H NMR analysis revealed the α configuration of SLMP1-1. Antioxidant assessments, including DPPH, ABTS, and ferric ion reduction assays, were detected with inhibitory values at 21.82–82.98%, 38.21–98.46%, and 3.30–20.30% at 0.2–1.0 mg/mL. This confirmed the effective antioxidant capacity of SLMP1-1, which is notably enhanced post oral and gastric digestion. The findings suggest that polysaccharides extracted from Schizochytrium limacinum meal hold significant promise as natural antioxidants.
Production of Biocalcium from Fermented Fish Bone Residue for Fish Emulsion Sausage Fortification
Authors: Gawborisut Somsamorn , Ketkaew Chavis , Buasook Thongsa
Published: March 2024

Fermented fish bone residue (FFBR) is an underused by-product of the industrial-scale production of fermented fish sauce. Subjecting FFBR to proper alkaline treatment can transform FFBR into biocalcium, which can be added to fish emulsion sausage (FES) to increase its calcium content. This study comprised two experiments. First, we aimed to find the most suitable alkaline treatment conditions for preparing biocalcium from FFBR. Alkaline treatments combining three sodium hydroxide (NaOH) concentrations (0%, 3%, and 6%) and three soaking times (0, 1, and 2 h) were tested. Quality parameters of alkaline-treated biocalcium (crude protein, crude fat, ash content, calcium, phosphorus, crude fiber, salt content, CIE color values, morphology of biocalcium particles, and the intensity of the fermented fish smell) were assessed. Second, we fortified FES with the properly treated biocalcium (0, 12, 24, or 36 g) and evaluated the sausage’s calcium, phosphorus, crude fiber, salt content, pH, CIE color values, texture profile analysis (TPA), emulsion stability, and sensory criteria. It was found that treatment with 3% or 6% NaOH produced better crude protein, ash content, calcium, and CIE color value results than no alkaline treatment. These two NaOH concentrations effectively lowered the salt content and the intensity of the fermented fish smell. However, 3% and 6% NaOH produced similar results. A soaking time of 1 h or 2 h produced better results than no soaking in terms of crude protein, crude fat, ash content, calcium, phosphorus, CIE color values, and the intensity of fermented fish smell. However, 1 h and 2 h produced similar results. It is concluded that 3% NaOH and soaking for 1 h would be the most suitable alkaline treatment to prepare biocalcium from FFBR. Fortifying FES with biocalcium from FFBR increased the calcium and phosphorus contents but slightly reduced TPA. The other FES quality parameters were unaffected by biocalcium fortification.
Anti-Inflammatory Activity of Peptides from Ruditapes philippinarum in Lipopolysaccharide-Induced RAW264.7 Cells and Mice
Authors: Lin Haisheng , Shen Weiqiang , Jiang Yu , Wu Qihang , Gao Jialong , Cao Wenhong , Zheng Huina , Chen Zhongqin , Zhong Saiyi , Qin Xiaoming
Published: March 2024

In our previous study, two peptides with favorable anti-inflammatory effects, Asp-Gln-Thr-Phe (DQTF) and Gly-Tyr-Thr-Arg (GYTR), were screened from Ruditapes philippinarum using an in vitro–in silico strategy. The present study aims to investigate the ameliorative effect of Ruditapes philippinarum peptides (RPPs) on acute inflammation and clarify the potential mechanism through in vitro and in vivo experiments. The anti-inflammatory effects of DQTF and GYTR were verified with a lipopolysaccharide (LPS)-induced RAW264.7 cell acute inflammation model and the anti-inflammatory effect of the enzymatic hydrolysates of Ruditapes philippinarum was explored in vivo using an LPS-induced acute inflammatory injury model in mice. The results show that DQTF and GYTR improved the morphology of LPS-injured cells and decreased the concentrations of tumor necrosis factor (TNF)-α and interleukin (IL)-6 in LPS-induced cells. Moreover, the antioxidant enzyme activity in cells was markedly increased with DQTF and GYTR. The enzymatic hydrolysates of Ruditapes philippinarum were obtained with hydrolysis using pepsin–chymotrypsin–trypsin (PeCTHC) and pepsin–trypsin (PeTHC), respectively. PeCTHC and PeTHC significantly reduced pro-inflammatory cytokines and nitric oxide (NO) in the serum. Additionally, the blood indices and levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and malondialdehyde (MDA) in the livers of mice were markedly improved with RPPs administration. In conclusion, RPPs have preventive and protective effects on acute inflammation, with significant prospects for development in the field of functional foods.
Associations between Milk Coagulation Properties and Microbiological Quality in Sheep Bulk Tank Milk
Authors: Arias Ramón , Jiménez Lorena , Garzón Ana , Caballero-Villalobos Javier , Oliete Bonastre , Amalfitano Nicolò , Cecchinato Alessio , Perea José M.
Published: March 2024

This study conducted a seasonal analysis of bulk tank milk from 77 sheep farms to establish relationships between the concentration of major microbial groups and milk coagulation properties. The investigated milk traits included composition (pH, fat, casein, lactose), coagulation properties (curd firmness: A60-, rennet clotting time: RCT-, curd firming time: k20-, curd yield: CY-), and somatic cell score (SCS). The main microbial groups analyzed were total mesophilic bacteria (SPC), thermodurics (THERMO), psychrotrophs (PSYCHRO), Pseudomonas spp. (PSEUDO), lactic acid bacteria (LAB), catalase-negative gram-positive cocci (GPCNC), Escherichia coli (ECOLI), coliforms other than Escherichia coli (COLI), coagulase-positive staphylococci (CPS), coagulase-negative staphylococci (CNS), and spores of lactate-fermenting Clostridium (BAB). Mixed linear models were used to explore associations between coagulation properties and the aforementioned variables. Results demonstrated that incorporating microbial loads into the models improves their fit and the relative quality of the outcomes. An important seasonality is demonstrated by an increase in CY and A60, along with a decrease in RCT and k20 during autumn and winter, contrasting with spring and summer. BAB concentration resulted in a reduction of A60 and an increase in RCT, whereas SPC concentration led to an enhancement of A60 and a reduction in RCT. An increase in GPCNC concentration was associated with an increase in k20 and a decrease in CY.
Mass Spectrometry Characterization of the SDS-PAGE Protein Profile of Legumins and Vicilins from Chickpea Seed
Authors: Di Francesco Antonella , De Santis Michele Andrea , Lanzoni Aldo , Pittalà Maria Gaetana Giovanna , Saletti Rosaria , Flagella Zina , Cunsolo Vincenzo
Published: March 2024

Chickpea (Cicer arietinum L.) seed proteins show a lot of functional properties leading this legume to be an interesting component for the development of protein-enriched foods. However, both the in-depth proteomic investigation and structural characterization of chickpea seed proteins are still lacking. In this paper a detailed characterization of chickpea seed protein fraction by means of SDS-PAGE, in-gel protein digestion, high-resolution mass spectrometry, and database searching is reported. Through this approach, twenty SDS gel bands were cut and analyzed. While the majority of the bands and the identified peptides were related to vicilin and legumin storage proteins, metabolic functional proteins were also detected. Legumins, as expected, were revealed at 45–65 kDa, as whole subunits with the α- and β-chains linked together by a disulphide bond, but also at lower mass ranges (α- and β-chains migrating alone). Similarly, but not expected, the vicilins were also spread along the mass region between 65 and 23 kDa, with some of them being identified in several bands. An MS structural characterization allowed to determine that, although chickpea vicilins were always described as proteins lacking cysteine residues, they contain this amino acid residue. Moreover, similar to legumins, these storage proteins are firstly synthesized as pre-propolypeptides (Mr 50–80 kDa) that may undergo proteolytic steps that not only cut the signal peptides but also produce different subunits with lower molecular masses. Overall, about 360 different proteins specific of the Cicer arietinum L. species were identified and characterized, a result that, up to the current date, represents the most detailed description of the seed proteome of this legume.
The Relationship between Food Healthiness, Trust, and the Intention to Reuse Food Delivery Apps: The Moderating Role of Eco-Friendly Packaging
Authors: Sun Kyung-A , Moon Joonho
Published: March 2024

The goal of this research is to investigate the relationship among food healthiness, trust, and the intention to reuse food delivery apps. Another purpose of this work is to examine the moderating effect of eco-friendly food packaging on the association between food healthiness and trust in food delivery apps. A survey was the main instrument for this work, with Amazon Mechanical Turk being used to collect the relevant data, resulting in a total of 343 observations. PROCESS model 7 was employed to test the research hypotheses. The results reveal that the intention to reuse is positively impacted by trust and food healthiness in food delivery apps. The results also uncover a significant moderating impact of eco-friendly packaging on the relationship between food healthiness and trust. The high food healthiness and high eco-friendly packaging group has the highest level of trust, while the low food healthiness and low eco-friendly packaging group has the lowest. The results of this research are therefore important because they clarify the relationship among these four attributes. Moreover, the results of this study have notable managerial implications.
Associations between Social Contact, Sleep and Dietary Patterns among Children: A Cross-Sectional Study
Authors: Mühlematter Christophe , Beaugrand Matthieu , Markovic Andjela , Kurth Salome
Published: March 2024

Social isolation in adults can be associated with altered sleep and eating behavior. This study aimed to investigate the interactions between the extent of social contact, eating behavior and sleep in infants and preschool children. In an observational study, 439 caregivers of 562 children aged 0–6 years provided information on sleep (i.e., duration, latency, bedtimes and nighttime awakenings), eating behaviors (i.e., meal size, consumption of sweet snacks, salty snacks, fruits and vegetables) and social contact (i.e., quarantine status, household size, social activities) during the COVID-19 pandemic (April 2020). In infants (0–3 years), the change in meal size and consumption of snacks, fruits, and vegetables did not significantly relate to the extent of social contact. For preschool children (3–6 years), a trend was observed, suggesting that quarantine status was associated with increased meal size. Changes in sleep duration, sleep latency, bedtimes and nighttime awakenings from before to during the pandemic were not significantly linked to the three variables quantifying social contact in both age groups. This study highlights that, contrary to expectations, the extent of social contact has negligible associations with infants’ and preschool children’s sleep and eating behaviors. These findings indicate that other factors beyond social isolation play a role in shaping children’s eating habits and sleep patterns.
Convergence Analysis of Iterative Learning Control for Initialized Fractional Order Systems
Authors: Xu Xiaofeng , Lu Jiangang , Chen Jinshui
Published: March 2024

Iterative learning control is widely applied to address the tracking problem of dynamic systems. Although this strategy can be applied to fractional order systems, most existing studies neglected the impact of the system initialization on operation repeatability, which is a critical issue since memory effect is inherent for fractional operators. In response to the above deficiencies, this paper derives robust convergence conditions for iterative learning control under non-repetitive initialization functions, where the bound of the final tracking error depends on the shift degree of the initialization function. Model nonlinearity, initial error, and channel noises are also discussed in the derivation. On this basis, a novel initialization learning strategy is proposed to obtain perfect tracking performance and desired initialization trajectory simultaneously, providing a new approach for fractional order system design. Finally, two numerical examples are presented to illustrate the theoretical results and their potential applications.
Accelerated Gradient Descent Driven by Lévy Perturbations
Authors: Chen Yuquan , Wu Zhenlong , Lu Yixiang , Chen Yangquan , Wang Yong
Published: March 2024

In this paper, we mainly consider two kinds of perturbed accelerated gradient descents driven by Lévy perturbations, which is of great importance for enhancing the global search ability. By using Lévy representation, Lévy perturbations can be divided into two parts: small jumps and large jumps, whose properties are then carefully discussed. By introducing the concept of attraction domain for local minima, Makovian transition properties are proven for the proposed two perturbed accelerated gradient descents with different infinitesimal matrices. Finally, all the results are extended to the vector case and two simulation examples are provided to validate all the conclusions.
The Risk Genes for Neuropsychiatric Disorders negr1 and opcml Are Expressed throughout Zebrafish Brain Development
Authors: Habicher Judith , Sanvido Ilaria , Bühler Anja , Sartori Samuele , Piccoli Giovanni , Carl Matthias
Published: March 2024

The immunoglobulin LAMP/OBCAM/NTM (IgLON) family of cell adhesion molecules comprises five members known for their involvement in establishing neural circuit connectivity, fine-tuning, and maintenance. Mutations in IgLON genes result in alterations in these processes and can lead to neuropsychiatric disorders. The two IgLON family members NEGR1 and OPCML share common links with several of them, such as schizophrenia, autism, and major depressive disorder. However, the onset and the underlying molecular mechanisms have remained largely unresolved, hampering progress in developing therapies. NEGR1 and OPCML are evolutionarily conserved in teleosts like the zebrafish (Danio rerio), which is excellently suited for disease modelling and large-scale screening for disease-ameliorating compounds. To explore the potential applicability of zebrafish for extending our knowledge on NEGR1- and OPCML-linked disorders and to develop new therapeutic strategies, we investigated the spatio-temporal expression of the two genes during early stages of development. negr1 and opcml are expressed maternally and subsequently in partially distinct domains of conserved brain regions. Other areas of expression in zebrafish have not been reported in mammals to date. Our results indicate that NEGR1 and OPCML may play roles in neural circuit development and function at stages earlier than previously anticipated. A detailed functional analysis of the two genes based on our findings could contribute to understanding the mechanistic basis of related psychiatric disorders.
Exploring Inpatients’ Perspective: A Cross-Sectional Survey on Satisfaction and Experiences in Greek Hospitals
Authors: Karaferis Dimitris Charalambos , Niakas Dimitris A.
Published: March 2024

Introduction: The aim of this study was to identify and evaluate patient-relevant experiences that fulfill the expectations and demands of society in Greece and those that could be improved by offering a better quality of care. The satisfaction of health service recipients is one of the key elements of the success of a health system. Methods: A cross-sectional survey was conducted to obtain data on satisfaction with hospitalization from patients admitted to 10 public hospitals in Athens between June 2019 and December 2021. Statistical analysis was applied to 57 items and 7 dimensions of patient satisfaction, namely waiting–arrival–admission, nursing staff, medical staff, other staff, service and quality of food, interior environment, and procedures. Results: A total of 3724 patients, aged ≥ 18 years, who had experienced hospitalization and agreed to participate in the study were included, the response rate of which was 93%. Patient satisfaction and experience with healthcare services provided by hospitals is moderate, with almost two-thirds of patients (67.38%) satisfied with the care they received. The encounter with the medical–nursing personnel (3.75/5) and other staff (4/5) were factors that positively affected patients’ overall satisfaction with hospitalization. However, there were some causes of dissatisfaction, mainly associated with waiting hours, easy access to medical services or services received in emergencies, delays of planned procedures (3.50/5), or problems with old facilities and equipment (3.56/5). Conclusion: Based on the patients’ judgment, the performance of hospitals was rated at a ‘tolerable’ level. Professionalism and the education of personnel led to a positive treatment outcome and improved the experience of patients to a good level. However, public hospitals continued to be underfunded and lacked strong support, which affected staff communication and responsiveness to patients’ requirements, while smart technologies and the simplification of procedures were not adopted to help staff provide a better quality of healthcare. The results suggest that there is plenty of room for improvement.
Virtual Consultation in Dermatology: Access Inequalities According to Socioeconomic Characteristics and the Place of Residence
Authors: Marco-Ibáñez Almudena , Aibar-Remón Carlos , Gamba-Cabezas Adriana , Maldonado Lina , Aguilar-Palacio Isabel
Published: March 2024

Virtual consultation has been implemented as a tool to improve the cooperation and coordination between primary care and other specialties. However, in its use in dermatology, inequities have been described. The aim of this study was to identify individual and geographical factors affecting the likelihood of accessing this resource. We conducted a cross-sectional study. The study population was individuals living in Aragón, a region in the north-east of Spain, who were users of the Aragon Health Service in 2021. To explore the differences in individual and area characteristics, between patients with virtual and non-virtual dermatology consultation, we performed bivariate analyses. To analyse the use of virtual consultation in dermatology, a multilevel methodology stratified by sex was developed. We analysed 39,174 dermatology visits, with 16,910 being virtual (43.17%). The rates of virtual consultation were higher in urban areas and the most affluent areas, for older persons, chronic complex patients and people with more advantageous socioeconomic positions. The best multilevel model conducted showed inequalities by socioeconomic position and area of residence. There are individual and area inequalities in the use of teledermatology. As this tool should improve equity of access, teledermatology interventions must address and adapt to the needs of the local patient population.
Psychometric Evaluation of the German Version of the Perceived Access to Healthcare Questionnaire in a Sample of Individuals with Rare Chronic Diseases
Authors: Wehrli Susanne , Dwyer Andrew A. , Landolt Markus A.
Published: March 2024

Access to healthcare is multifaceted and poses significant challenges for individuals with chronic and rare diseases (RDs). This study aimed to conduct a psychometric evaluation of the German version of the Perception of Access to Healthcare Questionnaire (PAHQ) among individuals with RDs. We conducted an evaluation of the PAHQ using a sample of 271 adults with an RD diagnosis. The 31-item instrument underwent evaluation including a comparison of three different confirmatory factor models (CFA). Subsequent steps involved item removal, reliability analysis (computation of Cronbach’s alpha), and analysis of criterion-related validity. The six-factor model showed the best fit to the data and was selected for further examination. Subsequently, six items were removed. Fit indices for the final model were acceptable. Cronbach’s alpha ranged from 0.75 to 0.91 for the six subscales, except for the availability subscale which exhibited the lowest value (0.64). In terms of criterion-related validity, different skills relating to the navigation of access dimensions were significantly correlated with corresponding PAHQ subscales, thus confirming validity. The capacity of the PAHQ to guide targeted interventions and facilitate cross-population comparisons positions it as a valuable instrument for advancing healthcare access research and promoting equitable access to care, particularly for individuals with rare and chronic diseases.
Nurses’ Perception of Tension, Stress, and Pressure before and during the COVID-19 Pandemic: A Multicenter Serbian Study
Authors: Santric-Milicevic Milena , Pavlekic Kristina , Bukumiric Zoran , Stevanovic Aleksandar , Nikolic Dejan , Matejic Bojana , Matanovic Dragana , Backovic Dusan , Tulic Goran , Lukic Relja , Zivanovic Dubravka , Radosavljevic Sofija , Milovanovic Vladimir , Zdujic Marija , Stankovic Sanja , Asanin Milika , Zdravkovic Marija , Tomasevic Ratko
Published: March 2024

The mental health of healthcare workers, especially the nursing staff in intensive care units, is crucial for the optimal functioning of healthcare systems during medical emergencies. This study implements a cross-sectional design to investigate the associations between nurses’ personal characteristics, workplace challenges, and job satisfaction with the increased perception of tension, stress, and pressure at the workplace (TSPW) before and during the COVID-19 pandemic. In 2021, we surveyed 4210 nurses from 19 intensive healthcare facilities in the capital of Serbia, Belgrade, and, at that time, collected data about their perceived TSPW before and during the COVID-19 pandemic. Our study identified six predictors of the increase in TSPW, as perceived by nurses: their work in COVID-19 infectious zones (OR = 1.446), exhaustion due to work under protective equipment (OR = 1.413), uncertainty and fear of infection (OR = 1.481), a high degree of superiors’ appreciation and respect (OR = 1.147), a high degree of patients’ attitudes (OR = 1.111), and a low degree of work autonomy (OR = 0.889). The study’s findings suggest that a solution to this issue is necessary to ensure that nurses are safe and able to alleviate the physical and mental strain that comes with prolonged use of protective equipment. Nurses on the frontline of the pandemic require better health protection, better conditions, and respect for their role. Strategies to promote mental health would help reduce nurses’ stress and increase job satisfaction.
Assessment of HPV Knowledge and Awareness among Students and Staff at IBB University, Niger State, Nigeria: Implications for Health Education and Prevention
Authors: Ogbolu Melvin Omone , Kozlovszky Miklós
Published: March 2024

In Nigeria, statistics reveal that there is a high rate of cervical cancer among women and a significant lack of awareness surrounding Human Papillomavirus (HPV), which poses a substantial risk of HPV infection. This cross-sectional survey, conducted at Ibrahim Badamasi Babangida (IBB) University, focuses on adapting and exploring the factors that influence a 20-item scale to measure HPV knowledge, evaluating knowledge-associated patterns and HPV-associated risk factors. We examined HPV vaccination rates, infection awareness, vaccine awareness, and the impact of ethnicity on HPV knowledge. Various validated forms were adapted to measure HPV awareness and knowledge. Non-parametric tests addressed non-normality. Data were presented using median and IQR and categorical data were frequency-based. Bivariate tests (Mann–Witney, Kruskal Wallis) explored knowledge-associated factors, while quantile regression (75th percentile) examined HPV knowledge factors. Variables were considered statistically significant at p < 0.05. The adapted 20-item knowledge scale revealed strong reliability (Cronbach’s alpha = 0.913), ensuring internal consistency. The median knowledge score was 0, with an interquartile range (IQR) of 0–5. Our findings revealed a significant lack of awareness and knowledge about HPV; only 34.8% of the population were aware of HPV infection and 25.0% were familiar with HPV vaccination. Furthermore, ethnicity was found to be significantly associated with knowledge of HPV. This study emphasizes the necessity for targeted interventions to enhance HPV awareness, especially within specific ethnic groups. Despite a robust knowledge scale, educational initiatives such as seminars/conferences about HPV and cervical cancer remain crucial in addressing this gap, ultimately reducing HPV infection and cervical cancer risks in Nigeria.
Preconception Health of Indigenous Peoples in Australia, Canada, New Zealand, and the United States: A Scoping Review
Authors: Walker Clara , Begum Tahmina , Boyle Jacqueline A , Ward James , Barzi Federica
Published: March 2024

Background: There is increasing recognition of the importance of the preconception period for addressing reproductive and intergenerational health inequities and supporting improved maternal and child health outcomes. This study aimed to understand the extent and type of evidence that exists in relation to preconception health for Indigenous peoples living in high-income countries with similar experiences of colonisation, namely, Australia, New Zealand, Canada, and the United States. Methods: This review was conducted as per the JBI methodology and PRISMA Extension for Scoping Reviews. A comprehensive search of PubMed, CINAHL [EBSCO], Ovid Embase, Scopus, and the Wiley Cochrane Library was conducted using keywords and index terms. We included research in English published between January 2010 and June 2023 on quantitative and qualitative primary studies. Data were extracted using a standardised tool, and the analysis included quantitative descriptions and qualitative content analysis. Results: We identified 360 potential studies and included 57 articles in the review. Most studies were from the United States (n = 36, 63.2%) and Australia (n = 13, 22.8%), and they commonly reported associations between preconception health risk factors and maternal or child health outcomes (n = 27, 48.2%) or described the development, implementation, or evaluation of preconception health interventions (n = 26, 46.4%). Common preconception health areas were pre-pregnancy body mass index or weight (n = 34), alcohol (n = 16), diet (n = 14), physical activity (n = 12), and diabetes (n = 11). Most studies focused exclusively on women (n = 46, 80.7%), and very few included men (n = 3, 5.3%). The study populations were mostly urban and rural (n = 25, 43.9%) or rural only (n = 14, 24.6%); however, the geographical remoteness was often unclear (n = 14, 24.6%). Conclusions: While there was some research relating to the preconception health of Indigenous peoples, this review identified considerable research gaps. There is a need for dedicated research into preconception health risk factors and reproductive health outcomes, attitudes and awareness of preconception health, and preconception health interventions for Indigenous peoples.
Evaluation of Qualitative Colour Palettes for Tactile Maps
Authors: Wabiński Jakub , Śmiechowska-Petrovskij Emilia
Published: March 2024

Much attention is currently being paid to developing universally designed solutions. Tactile maps, designed for people with visual impairments (PVI), require both graphic and tactile content. While many more- or less-official guidelines regarding tactile symbols exist, the subject literature lacks clear guidance on creating legible, highly contrasting graphic symbols for visual perception by those with residual vision. This study specifically addresses the application of colour, a key graphic variable that Is most often used to differentiate area symbols. We wanted to verify whether it is possible to choose a universal qualitative colour palette for tactile maps. We have proposed four different palettes, each with eight colours, that were later evaluated in a controlled study by 16 PVI with varying sociodemographic characteristics, using the VIEW model. The model is widely applied in the area of marketing research and considers the following aspects: Visibility, Informational, Emotional Appeal, and Workability. Our results indicate a lack of unanimity in choosing the best qualitative palette. The results of three palettes are comparable, with a subtle preference for the palette optimized for colour differences using the Python algorithm. Notably, the palette commonly used in official tactile maps in Poland received the lowest scores in every analysed dimension.
Whole-Genome Survey Analyses of Five Goby Species Provide Insights into Their Genetic Evolution and Invasion-Related Genes
Authors: Ma Siyu , Zhao Xiang , Song Na
Published: March 2024

As one of the most abundant groups in marine fish families, Gobiidae fish are important fishery resources in China, and some are also invasive species in certain regions worldwide. However, the phylogenetic relationships of Gobiidae fish remain ambiguous, and the study of their invasion-related genes is still scarce. This study used high-throughput sequencing technology to conduct a whole-genome survey of five Gobiidae fish species: Acanthogobius flavimanus, Acanthogobius stigmothonus, Favonigobius gymnauchen, Ctenotrypauchen microcephalus, and Tridentiger barbatus. De novo assembly of five fish genomes was performed, and genomic traits were compared through K-mer analysis. Among the five Gobiidae fish genomes, F. gymnauchen had the largest genome size (1601.98 Mb) and the highest heterozygosity (1.56%) and repeat rates (59.83%). Phylogenetic studies showed that A. flavimanus was most closely linked to A. stigmothonus, while Apogonidae and Gobiidae were closely related families. PSMC analysis revealed that C. microcephalus experienced a notable population expansion than the other four fish species in the Early Holocene. By using the KOG, GO, and KEGG databases to annotate single-copy genes, the annotated genes of the five fish were mainly classified as “signal transduction mechanisms”, “cellular process”, “cellular anatomical entity”, and “translation”. Acanthogobius flavimanus, A. stigmothonus, and T. barbatus had more genes classified as “response to stimulus” and “localization”, which may have played an important role in their invasive processes. Our study also provides valuable material about Gobiidae fish genomics and genetic evolution.
QTL Mapping and Data Mining to Identify Genes Associated with Soybean Epicotyl Length Using Cultivated Soybean and Wild Soybean
Authors: Chen Lin , Ma Shengnan , Li Fuxin , Li Lanxin , Yu Wenjun , Yu Lin , Tang Chunshuang , Liu Chunyan , Xin Dawei , Chen Qingshan , Wang Jinhui
Published: March 2024

Soybean (Glycine max) plants first emerged in China, and they have since been established as an economically important oil crop and a major source of daily protein for individuals throughout the world. Seed emergence height is the first factor that ensures seedling adaptability to field management practices, and it is closely related to epicotyl length. In the present study, the Suinong 14 and ZYD00006 soybean lines were used as parents to construct chromosome segment substitution lines (CSSLs) for quantitative trait loci (QTL) identification. Seven QTLs were identified using two years of epicotyl length measurement data. The insertion region of the ZYD00006 fragment was identified through whole genome resequencing, with candidate gene screening and validation being performed through RNA-Seq and qPCR, and Glyma.08G142400 was ultimately selected as an epicotyl length-related gene. Through combined analyses of phenotypic data from the study population, Glyma.08G142400 expression was found to be elevated in those varieties exhibiting longer epicotyl length. Haplotype data analyses revealed that epicotyl data were consistent with haplotype typing. In summary, the QTLs found to be associated with the epicotyl length identified herein provide a valuable foundation for future molecular marker-assisted breeding efforts aimed at improving soybean emergence height in the field, with the Glyma.08G142400 gene serving as a regulator of epicotyl length, offering new insight into the mechanisms that govern epicotyl development.
A Comparative Investigation of the Bile Microbiome in Patients with Choledocholithiasis and Cholecystolithiasis through Metagenomic Analysis
Authors: Park Wonsuk , Park Joonhong
Published: March 2024

While the precise triggers of gallstone formation remain incompletely understood, it is believed to arise from a complex interplay of genetic and environmental factors. The bile microbiome is being increasingly recognized as a possible contributor to the onset of gallstone disease. The primary objective of this study was to investigate distinctions in the microbial communities within bile specimens from patients with choledocholithiasis (common bile duct stones) and cholecystolithiasis (gallbladder stones). We employed massively parallel sequencing of the 16S rRNA gene to examine the microbial communities within bile samples obtained from 28 patients with choledocholithiasis (group DS) and cholecystolithiasis (group GS). The taxonomic composition of the bile microbial communities displayed significant disparities between the group DS and the group GS. Within the 16 prevalent genera, only Streptococcus, Ralstonia, Lactobacillus, and Enterococcus were predominantly found in the group GS. In contrast, the group DS displayed a more diverse range of genera. The alpha diversity of bile specimens was also notably lower in the group GS compared to the group DS (p = 0.041). Principal coordinate analysis unveiled distinct clustering of bile microbial communities depending on the location of the gallstone. Linear discriminant analysis effect size analysis, with a score threshold of >3 and the Kruskall–Wallis test (α < 0.05), recognized Bacilli and Lactobacillales as potential taxonomic markers for distinguishing patients with cholecystolithiasis limited to the gallbladder. Significant variations were found in the distribution and diversity of bile microbial communities between patients with choledocholithiasis and cholecystolithiasis. This observation suggests that alterations in the bile microbiome may contribute to the development of gallstones in these patients.
The Renin–Angiotensin System and Cardiovascular–Kidney–Metabolic Syndrome: Focus on Early-Life Programming
Authors: Tain You-Lin , Hsu Chien-Ning
Published: March 2024

The identification of pathological links among metabolic disorders, kidney ailments, and cardiovascular conditions has given rise to the concept of cardiovascular–kidney–metabolic (CKM) syndrome. Emerging prenatal risk factors seem to increase the likelihood of CKM syndrome across an individual’s lifespan. The renin–angiotensin system (RAS) plays a crucial role in maternal–fetal health and maintaining homeostasis in cardiovascular, metabolic, and kidney functions. This review consolidates current preclinical evidence detailing how dysregulation of the RAS during pregnancy and lactation leads to CKM characteristics in offspring, elucidating the underlying mechanisms. The multi-organ effects of RAS, influencing fetal programming and triggering CKM traits in offspring, suggest it as a promising reprogramming strategy. Additionally, we present an overview of interventions targeting the RAS to prevent CKM traits. This comprehensive review of the potential role of the RAS in the early-life programming of CKM syndrome aims to expedite the clinical translation process, ultimately enhancing outcomes in cardiovascular–kidney–metabolic health.
Post-Traumatic Expressions of Aromatase B, Glutamine Synthetase, and Cystathionine-Beta-Synthase in the Cerebellum of Juvenile Chum Salmon, Oncorhynchus keta
Authors: Pushchina Evgeniya V. , Bykova Mariya E. , Varaksin Anatoly A.
Published: March 2024

In adult fish, neurogenesis occurs in many areas of the brain, including the cerebellum, with the ratio of newly formed cells relative to the total number of brain cells being several orders of magnitude greater than in mammals. Our study aimed to compare the expressions of aromatase B (AroB), glutamine synthetase (GS), and cystathionine-beta-synthase (CBS) in the cerebellum of intact juvenile chum salmon, Oncorhynchus keta. To identify the dynamics that determine the involvement of AroB, GS, and CBS in the cellular mechanisms of regeneration, we performed a comprehensive assessment of the expressions of these molecular markers during a long-term primary traumatic brain injury (TBI) and after a repeated acute TBI to the cerebellum of O. keta juveniles. As a result, in intact juveniles, weak or moderate expressions of AroB, GS, and CBS were detected in four cell types, including cells of the neuroepithelial type, migrating, and differentiated cells (graphic abstract, A). At 90 days post injury, local hypercellular areas were found in the molecular layer containing moderately labeled AroB+, GS+, and CBS+ cells of the neuroepithelial type and larger AroB+, GS+, and CBS+ cells (possibly analogous to the reactive glia of mammals); patterns of cells migration and neovascularization were also observed. A repeated TBI caused the number of AroB+, GS+, and CBS+ cells to further increase; an increased intensity of immunolabeling was recorded from all cell types (graphic abstract, C). Thus, the results of this study provide a better understanding of adult neurogenesis in teleost fishes, which is expected to clarify the issue of the reactivation of adult neurogenesis in mammalian species.
Differential Gene Regulatory Network Analysis between Azacitidine-Sensitive and -Resistant Cell Lines
Authors: Park Heewon , Miyano Satoru
Published: March 2024

Azacitidine, a DNA methylation inhibitor, is employed for the treatment of acute myeloid leukemia (AML). However, drug resistance remains a major challenge for effective azacitidine chemotherapy, though several studies have attempted to uncover the mechanisms of azacitidine resistance. With the aim to identify the mechanisms underlying acquired azacitidine resistance in cancer cell lines, we developed a computational strategy that can identify differentially regulated gene networks between drug-sensitive and -resistant cell lines by extending the existing method, differentially coexpressed gene sets (DiffCoEx). The technique specifically focuses on cell line-specific gene network analysis. We applied our method to gene networks specific to azacitidine sensitivity and identified differentially regulated gene networks between azacitidine-sensitive and -resistant cell lines. The molecular interplay between the metallothionein gene family, C19orf33, ELF3, GRB7, IL18, NRN1, and RBM47 were identified as differentially regulated gene network in drug resistant cell lines. The biological mechanisms associated with azacitidine and AML for the markers in the identified networks were verified through the literature. Our results suggest that controlling the identified genes (e.g., the metallothionein gene family) and “cellular response”-related pathways (“cellular response to zinc ion”, “cellular response to copper ion”, and “cellular response to cadmium ion”, where the enriched functional-related genes are MT2A, MT1F, MT1G, and MT1E) may provide crucial clues to address azacitidine resistance in patients with AML. We expect that our strategy will be a useful tool to uncover patient-specific molecular interplay that provides crucial clues for precision medicine in not only gastric cancer but also complex diseases.
Dermatologic Manifestations of Mitochondrial Dysfunction: A Review of the Literature
Authors: Natarelli Nicole , Gahoonia Nimrit , Aflatooni Shaliz , Bhatia Sahibjot , Sivamani Raja K.
Published: March 2024

Mitochondria are eukaryotic cellular organelles that function in energy metabolism, ROS production, and programmed cell death. Cutaneous epithelial and hair follicle dermal papilla cells are energy-rich cells that thereby may be affected by mitochondrial dysfunction and DNA mutation accumulation. In this review, we aimed to summarize the medical literature assessing dermatologic conditions and outcomes associated with mitochondrial dysfunction. A search of PubMed and Embase was performed with subsequent handsearching to retrieve additional relevant articles. Mitochondrial DNA (mtDNA) deletions, mutation accumulation, and damage are associated with phenotypic signs of cutaneous aging, hair loss, and impaired wound healing. In addition, several dermatologic conditions are associated with aberrant mitochondrial activity, such as systemic lupus erythematosus, psoriasis, vitiligo, and atopic dermatitis. Mouse model studies have better established causality between mitochondrial damage and dermatologic outcomes, with some depicting reversibility upon restoration of mitochondrial function. Mitochondrial function mediates a variety of dermatologic conditions, and mitochondrial components may be a promising target for therapeutic strategies.