bluuebunny/crossref_metadata_2025 huggingface dataset:
Multiple sclerosis: reduced proportion of circulating plasmacytoid dendritic cells expressing BDCA-2 and BDCA-4 and reduced production of IL-6 and IL-10 in response to herpes simplex virus type 1
Authors: Sanna A , Huang YM , Arru G , Fois ML , Link H , Rosati G , Sotgiu S
Published: July 2008

Objective

We hypothesized that autoaggressive immune responses observed in multiple sclerosis (MS) could be associated with an imbalance in proportion of immune cell subsets and in cytokine production in response to infection, including viruses.

Methods

We collected blood mononuclear cells (MNC) from 23 patients with MS and 23 sex- and age-matched healthy controls (HC) from the island of Sardinia, Italy, where the prevalence of MS is extraordinarily high. Using flow cytometry, we studied MNC for expression of blood dendritic cell antigens (BDCA)-2 and BDCA-4 surface markers reflecting the proportion of plasmacytoid dendritic cells (pDC) that produce type I interferons (IFNs) after virus challenge and promote Th2/anti-inflammtory cytokine production. In parallel, pro-inflammatory (interleukin [IL]-2, IL-12, IFN-γ), anti-inflammatory (IL-4, IL-10), and immuno-regulatory/pleiotropic cytokines (type I IFNs including IFN-α and β, IL-6) were measured before and after an in vitro exposure to herpes simplex virus type 1 (HSV-1).

Results

The subset of lineage negative (lin−), BDCA-2+ cells was lower in patients with MS compared with HC (0.08 ± 0.02% vs 0.24 ± 0.02%; P < 0.001). A similar pattern was observed for lin−BDCA-4+ cells (0.08 ± 0.02% vs 0.17% ± 0.03; P < 0.01). Spontaneous productions of IL-6 (45 ± 10 pg/mL vs 140 ± 26 pg/mL; P < 0.01) and IL-10 (17 ± 0.4 pg/mL vs 21 ± 1 pg/mL; P < 0.05) by MNC were lower in patients with MS compared with HC. Spontaneous production of IL-6 (6.5 ± 0.15 pg/mL vs 21 ± 5 pg/mL; P < 0.01 and IL-10 (11 ± 1 pg/mL vs 14 ± 3 pg/mL; P < 0.05) by pDC was also lower in patients with MS compared with HC. Exposure of MNC to HSV-1 showed, in both patients with MS and HC, increased production of IFN-α, IL-6, and IL-10 but decreased production of IL-4. In response to HSV-1 exposure, productions of IL-6 (165 ± 28 pg/mL vs 325 ± 35 pg/mL; P < 0.01) and IL-10 (27 ± 3 vs 33 ± 3 P < 0.05) by MNC as well as by pDC (IL-6: 28 ± 7 vs 39 ± 12 P < 0.05; IL-10: 14 ± 1 vs 16 ± 3 P < 0.05) were lower in patients with MS compared with HC.

Conclusion

The results implicate a new evidence for altered immune cells and reduced immune responses in response to viral challenge in MS.
Seeking a usable methodology: The production of the fin-de-siècle female prostitute
Authors: Jansdotter Anna
Published: July 2008

The idea of a usable methodology, one which will both retain the idea of `a subject' at the heart of research and also recognize that in historical research the researcher necessarily has to work with textual remains, is discussed. Piecing this together is explored in relation to researching correspondences and other archival materials relating to two women who were fin de siècle prostitutes and also a deaconess who helped and `rescued' them.
Surviving in a Man's World with a Sense of Humour: An Analysis of Women Leaders' Use of Humour at Work
Authors: Schnurr Stephanie
Published: July 2008

Communication not only constitutes one of the crucial aspects of leadership performance, but leadership can productively be viewed as a communication process. Humour is one of the prime means which enables leaders to achieve their various transactional as well as relational objectives. This article aims at exploring some of the ways in which women leaders make use of this particularly versatile discursive strategy in order to enhance their leadership performance while also resolving the challenges of being the `odd girls out' in a predominantly masculine work environment. Drawing on authentic discourse data collected in two New Zealand organizations, and pursuing a discourse-analytical approach, this article illustrates that women leaders skilfully employ humour to portray themselves as effective leaders while at the same time negotiating and performing their gender identities in a masculine domain. Findings also indicate the advantages of a discourse-analytical approach for an assessment of the complexities of the leadership process.
Peroxisome proliferator-activated receptor-α reduces inflammation and vascular leakage in a murine model of acute lung injury
Authors: Schaefer M. B. , Pose A. , Ott J. , Hecker M. , Behnk A. , Schulz R. , Weissmann N. , Günther A. , Seeger W. , Mayer K.
Published: July 2008

Acute lung injury (ALI) still represents a major cause of morbidity and mortality in intensive care units. Beneficial effects have been described after activation of the peroxisome proliferator-activated receptor (PPAR)-α by fibrates such as WY 14,643 (WY) in inflammatory models. In the present study, the impact of WY was investigated in a model of endotoxin (lipopolysaccharide; LPS)-induced ALI in mice.

Intratracheal LPS challenge dose-dependently resulted in leukocyte invasion, protein leakage and release of tumour necrosis factor-α as well as macrophage inflammatory protein-2, prostaglandin E2and thromboxane B2into the alveolar space after 8 and 24 h. Lung ventilator compliance was reduced at both time-points. In isolated perfused mouse lungs, platelet-activating factor (PAF) induced an acute increase in pulmonary artery pressure (Ppa) and in capillary filtration coefficient (Kfc). WY significantly improved all features of ALIin vivoand blunted the increase inKfcin isolated perfused mice lungs. In mice with genetic deletion of PPAR-α, all characteristics of ALI,Ppa, andKfcwere not significantly different from wild-type mice but WY failed to improve ALI and PAF-induced increase inKfc.

Activation of peroxisome proliferator-activated receptor-α by WY 14,643 reduced acute lung injury and vascular leakage. Fibrates may possess beneficial effects in acute pulmonary diseases beyond their lipid-lowering capability.
The epidermal growth factor receptor mediates allergic airway remodelling in the rat
Authors: Tamaoka M. , Hassan M. , McGovern T. , Ramos-Barbón D. , Jo T. , Yoshizawa Y. , Tolloczko B. , Hamid Q. , Martin J. G.
Published: July 2008

The chronicity of bronchial asthma is attributed to persistent airway inflammation and to a variety of structural changes, or remodelling, that includes smooth muscle and goblet cell hyperplasia.

To investigate the mechanisms of airway remodelling, the current authors used an established allergen (ovalbumin; OVA)-driven rodent model (the Brown Norway rat).

Brown Norway rats were sensitised to OVA and challenged three times at 5-day intervals to evoke airway remodelling. The effects of an epidermal growth factor (EGF) receptor inhibitor, AG1478, and a cysteinyl leukotriene-1 receptor antagonist, montelukast, on epithelial and airway smooth muscle (ASM) cell proliferationin vivoin response to repeated OVA challenge were tested. Three challenges with leukotriene (LT)D4were given, to examine their effects on remodelling with and without AG1478 pretreatment.

OVA challenges caused ASM hyperplasia, with an increase in mass, epithelial cell proliferation and goblet cell proliferation. AG1478 prevented the changes, as did montelukast. Multiple OVA challenges increased heparin-binding EGF-like growth factor but not EGF expression by airway epithelium. LTD4reproduced the changes in remodelling induced by OVA and this was blocked by AG1478.

Allergen-induced airway epithelial and airway smooth muscle remodelling is mediated by cysteinyl leukotrienesviathe cysteinyl leukotriene-1 receptor with downstream effects on the epidermal growth factor receptor axis.
RNA interference for CFTR attenuates lung fluid absorption at birth in rats
Authors: Li Tianbo , Koshy Shyny , Folkesson Hans G
Published: July 2008

Background

Small interfering RNA (siRNA) against αENaC (α-subunit of the epithelial Na channel) and CFTR (cystic fibrosis transmembrane conductance regulator) was used to explore ENaC and CTFR function in newborn rat lungs.

Methods

Twenty-four hours after trans-thoracic intrapulmonary (ttip) injection of siRNA-generating plasmid DNA (pSi-0, pSi-4, or pSi-C2), we measured CFTR and ENaC expression, extravascular lung water, and mortality.

Results

αENaC and CFTR mRNA and protein decreased by ~80% and ~85%, respectively, following αENaC and CFTR silencing. Extravascular lung water and mortality increased after αENaC and CFTR-silencing. In pSi-C2-transfected isolated DLE cells there were attenuated CFTR mRNA and protein. In pSi-4-transfected DLE cells αENaC mRNA and protein were both reduced. Interestingly, CFTR-silencing also reduced αENaC mRNA and protein. αENaC silencing, on the other hand, only slightly reduced CFTR mRNA and protein.

Conclusion

Thus, ENaC and CFTR are both involved in the fluid secretion to absorption conversion around at birth.
New steroidal aromatase inhibitors: Suppression of estrogen-dependent breast cancer cell proliferation and induction of cell death
Authors: Cepa Margarida , Correia-da-Silva Georgina , da Silva Elisiário J Tavares , Roleira Fernanda MF , Borges Margarida , Teixeira Natércia A
Published: July 2008

Background

Aromatase, the cytochrome P-450 enzyme (CYP19) responsible for estrogen biosynthesis, is an important target for the treatment of estrogen-dependent breast cancer. In fact, the use of synthetic aromatase inhibitors (AI), which induce suppression of estrogen synthesis, has shown to be an effective alternative to the classical tamoxifen for the treatment of postmenopausal patients with ER-positive breast cancer. New AIs obtained, in our laboratory, by modification of the A and D-rings of the natural substrate of aromatase, compounds 3a and 4a, showed previously to efficiently suppress aromatase activity in placental microsomes. In the present study we have investigated the effects of these compounds on cell proliferation, cell cycle progression and induction of cell death using the estrogen-dependent human breast cancer cell line stably transfected with the aromatase gene, MCF-7 aro cells.

Results

The new steroids inhibit hormone-dependent proliferation of MCF-7aro cells in a time and dose-dependent manner, causing cell cycle arrest in G0/G1 phase and inducing cell death with features of apoptosis and autophagic cell death.

Conclusion

Our in vitro studies showed that the two steroidal AIs, 3a and 4a, are potent inhibitors of breast cancer cell proliferation. Moreover, it was also shown that the antiproliferative effects of these two steroids on MCF-7aro cells are mediated by disrupting cell cycle progression, through cell cycle arrest in G0/G1 phase and induction of cell death, being the dominant mechanism autophagic cell death. Our results are important for the elucidation of the cellular effects of steroidal AIs on breast cancer.
Conjugated linoleic acid induces apoptosis through estrogen receptor alpha in human breast tissue
Authors: Wang Li-Shu , Huang Yi-Wen , Liu Suling , Yan Pearlly , Lin Young C
Published: July 2008

Background

Conjugated linoleic acid (CLA), a naturally occurring fatty acid found in ruminant products such as milk and beef, has been shown to possess anti-cancer activities inin vivoanimal models andin vitrocell culture systems. In human breast cancer, the overall duration of estrogen exposure is the most important risk factor for developing estrogen-responsive breast cancer. Accordingly, it has been suggested that estrogen exposure reduces apoptosis through the up-regulation of the anti-apoptosis protein, Bcl-2. Bcl-2, an anti-apoptotic protein, regulates apoptosis and plays a crucial role in the development and growth regulation of normal and cancerous cells. Our research interest is to examine the effects of CLA on the induction of apoptosis in human breast tissues.

Methods

The localization of Bcl-2 in both normal and cancerous human breast tissues was determined by immunohistochemical staining and the Bcl-2 protein expression was tested by western blot analysis. Co-culture of epithelial cells and stromal cells was carried out in the presence or absence of CLA to evaluate apoptosis in the context of a cell-cell interaction.

Results

The results showed that both normal and cancerous breast tissues were positive for Bcl-2 staining, which was higher overall in mammary ducts but very low in the surrounding stromal compartment. Interestingly, by quantifying the western blot data, basal Bcl-2 protein levels were higher in normal breast epithelial cells than in cancerous epithelial cells. Furthermore, treatment with 17β-estradiol (E2) stimulated growth and up-regulated Bcl-2 expression in estrogen responsive breast epithelial cells; however, these carcinogenic effects were diminished by either CLA or 4-Hydroxytamoxifen (Tam) and were suppressed further by the combination of CLA and Tam. In both one cell type cultured and co-culture systems, CLA induced cell apoptosis in ERα transfected MDA-MB-231 cells but not in the wild type MDA-MB-231 cells.

Conclusion

These data, therefore, demonstrate that ERα plays important roles in CLA induced apoptosis in human breast tissues.
Apoptotic pathways in pancreatic ductal adenocarcinoma
Authors: Hamacher Rainer , Schmid Roland M , Saur Dieter , Schneider Günter
Published: July 2008

Pancreatic ductal adenocarcinoma (PDAC) is one of the most common causes of cancer related death. Despite the advances in understanding of the molecular pathogenesis, pancreatic cancer remains a major unsolved health problem. Overall, the 5-year survival rate is less than 5% demonstrating the insufficiency of current therapies.

Most cytotoxic therapies induce apoptosis and PDAC cells have evolved a plethora of molecular mechanisms to assure survival. We will present anti-apoptotic strategies working at the level of the death receptors, the mitochondria or involving the caspase inhibitors of the IAP family. Furthermore, the survival function of the phosphotidylinositol-3' kinase (PI3K)/AKT- and NF-kappaB-pathways are illustrated. A detailed molecular knowledge of the anti-apoptotic mechanisms of PDAC cells will help to improve therapies for this dismal disease and therapeutic strategies targeting the programmed cell death machinery are in early preclinical and clinical development.
6-sulfo sialyl Lewis X is the common receptor determinant recognized by H5, H6, H7 and H9 influenza viruses of terrestrial poultry
Authors: Gambaryan Alexandra S , Tuzikov Alexander B , Pazynina Galina V , Desheva Julia A , Bovin Nicolai V , Matrosovich Mikhail N , Klimov Alexander I
Published: July 2008

Background

Influenza A viruses of domestic birds originate from the natural reservoir in aquatic birds as a result of interspecies transmission and adaptation to new host species. We previously noticed that influenza viruses isolated from distinct orders of aquatic and terrestrial birds may differ in their fine receptor-binding specificity by recognizing the structure of the inner parts of Neu5Acα2-3Gal-terminated sialyloligosaccharide receptors. To further characterize these differences, we studied receptor-binding properties of a large panel of influenza A viruses from wild aquatic birds, poultry, pigs and horses.

Results

Using a competitive solid-phase binding assay, we determined viral binding to polymeric conjugates of sialyloligosaccharides differing by the type of Neu5Acα-Gal linkage and by the structure of the more distant parts of the oligosaccharide chain. Influenza viruses isolated from terrestrial poultry differed from duck viruses by an enhanced binding to sulfated and/or fucosylated Neu5Acα2-3Gal-containing sialyloligosaccharides. Most of the poultry viruses tested shared a high binding affinity for the 6-sulfo sialyl Lewis X (Su-SLex). Efficient binding of poultry viruses to Su-SLex was often accompanied by their ability to bind to Neu5Acα2-6Gal-terminated (human-type) receptors. Such a dual receptor-binding specificity was demonstrated for the North American and Eurasian H7 viruses, H9N2 Eurasian poultry viruses, and H1, H3 and H9 avian-like virus isolates from pigs.

Conclusion

Influenza viruses of terrestrial poultry differ from ancestral duck viruses by enhanced binding to sulfated and/or fucosylated Neu5Acα2-3Gal-terminated receptors and, occasionally, by the ability to bind to Neu5Acα2-6Gal-terminated (human-type) receptors. These findings suggest that the adaptation to receptors in poultry can enhance the potential of an avian virus for avian-to-human transmission and pandemic spread.
Achalasia: unusual cause of chronic cough in children
Authors: Mehdi Nighat F , Weinberger Miles M , Abu-Hasan Mutasim N
Published: July 2008

Achalasia is a rare motility disorder of the esophagus which results from lack of enervation of the lower esophageal sphincter muscles and leads to dilatation of proximal esophagus. Patients with achalasia presents typically with dysphagia, vomiting of undigested food and failure to thrive. Cough can be present in achalasia patients due to aspiration of food or due to airway compression by the dilated esophagus. We report two cases of achalasia presenting primarily with prolonged cough. Diagnosis of achalasia in both cases was delayed due to this atypical presentation. This highlights the importance of recognizing achalasia as a potential cause of chronic cough in order to avoid delayed diagnosis and mismanagement.
Critical role of Lck in L-selectin signaling induced by sulfatides engagement
Authors: Xu Ting , Chen Liang , Shang Xin , Cui Lingling , Luo Jixian , Chen Cuixia , Ba Xueqing , Zeng Xianlu
Published: July 2008

Recruitment of leukocytes onto inflamed tissues is an important physiological event, in which L-selectin plays an essential role in initial leukocyte capture and at the same time, triggers cell signaling. Lck is a member of the Src family of protein tyrosine kinases and is critical for T cell activation triggered by receptor ligation. Here, we demonstrated that Lck was associated directly with and phosphorylated the L-selectin cytoplasmic tail upon L-selectin engagement with sulfatides. Through the direct interaction with ZAP-70 and c-Abl via its Src homology 2 (SH2) and SH3 domains, Lck organized a signaling complex at the cytoplasmic tail of L-selectin. In the cells with Lck knockdown by small interfering RNA treatment, L-selectin signaling was suppressed dramatically, as indicated by reduced phosphorylation of c-Abl and ZAP-70. Re-expression of wild-type or constitutively active but not kinase-dead murine Lck rescued the phosphorylation completely, but the SH2 domain mutant or the SH3/SH2 double mutant of murine Lck had no effect. These results suggest that Lck plays a critical role in L-selectin signaling upon sulfatides stimulation.
Viral replication and paracrine effects result in distinct, functional responses of dendritic cells following infection with dengue 2 virus
Authors: Nightingale Zachary D , Patkar Chinmay , Rothman Alan L
Published: July 2008

Dengue virus (DENV), a re-emerging arbovirus, readily infects dendritic cells (DC) in culture and in vivo. However, there have been contradictory reports regarding the effect of DENV infection on DC activation and maturation. DC undergo a series of functional changes following exposure to infectious agents, including cytokine production and costimulatory and MHC molecule induction, culminating in stimulation of adaptive immune responses. Immunological memory to primary DENV infection critically influences disease severity during subsequent infections with heterologous serotypes. To explore these phenomena, we examined DENV infection-dependent and -independent effects on DC secretory, phenotypic, and allostimulatory functions. DENV infection of DC resulted in the secretion of a broad array of cytokines and chemokines. Type I IFN produced by DC inhibited propagation of infection and induced the chemokine IFN-γ-inducible protein 10 (IP-10; CXCL10). Based on intracellular cytokine staining, infected DC produced less IP-10 but more TNF-α than uninfected bystander cells in the same culture. DENV exposure activated surface molecule expression on infected and bystander cells; infected DC had enhanced programmed death ligand 2 (PD-L2) and MHC II expression but reduced levels of PD-L1, CD80, CD86, and MHC I relative to bystander DC. Dengue-infected DC cultures stimulated resting allogeneic CD4 T cell proliferation, although an increasing multiplicity of infection was associated with decreasing stimulatory capacity of DC. These data demonstrate that functional maturation of DC in response to dengue infection is modified by the presence of virus through IFN-dependent and -independent mechanisms with consequences for the development of adaptive immunity.
IL-15-induced CD8+CD122+ T cells increase antibacterial and anti-tumor immune responses: implications for immune function in aged mice
Authors: Motegi Akira , Kinoshita Manabu , Inatsu Akihito , Habu Yoshiko , Saitoh Daizoh , Seki Shuhji
Published: July 2008

We previously proposed that mouse CD8+CD122+ T cells and human CD57+ T cells, which increase with age and exhibit potent IFN-γ production, represent a double-edged sword as they play critical roles in host defense and the lethal IL-12/LPS-induced generalized Shwartzman reaction (GSR). However, our proposal was based solely on comparisons of young and old mice. In this study, we attempted to increase CD8+CD122+ T cells in young mice with exogenous IL-15 and confirm their countervailing functions in young mice. After young mice (6 weeks) were injected with IL-15, they showed significant increases in CD8+CD122+ T cells in the liver and spleen. Liver CD8+CD122+ T cells from IL-15-pretreated mice had a potent capacity to produce IFN-γ after IL-12 injection or Escherichia coli infection. IL-15-pretreated mice showed increased survival to E. coli infections and enhanced anti-tumor activities against liver metastatic EL4 cells, as well as an exacerbation of the GSR. Correspondingly, liver CD8+CD122+ T cells produced more perforin than CD8+CD122− T cells in EL4-inoculated mice. Unexpectedly, comparable IL-15 treatment did not induce further increases in CD8+CD122+ T cells in aged mice and did not enhance their defenses against bacterial infection or tumor growth. Interestingly, however, nontreated, aged mice (50 weeks) showed twofold higher IL-15 levels (but not TNF or IFN-γ) in liver homogenates compared with young mice. Our results further support that CD8+CD122+ T cells, which are increased physiologically or therapeutically by IL-15, are involved in antibacterial immunity, anti-tumor immunity, and the GSR.
Pathfinding in a large vertebrate axon tract: isotypic interactions guide retinotectal axons at multiple choice points
Authors: Pittman Andrew J. , Law Mei-Yee , Chien Chi-Bin
Published: July 2008

Navigating axons respond to environmental guidance signals, but can also follow axons that have gone before - pioneer axons. Pioneers have been studied extensively in simple systems, but the role of axon-axon interactions remains largely unexplored in large vertebrate axon tracts, where cohorts of identical axons could potentially use isotypic interactions to guide each other through multiple choice points. Furthermore, the relative importance of axon-axon interactions compared with axon-autonomous receptor function has not been assessed. Here, we test the role of axon-axon interactions in retinotectal development, by devising a technique to selectively remove or replace early-born retinal ganglion cells (RGCs). We find that early RGCs are both necessary and sufficient for later axons to exit the eye. Furthermore,introducing misrouted axons by transplantation reveals that guidance from eye to tectum relies heavily on interactions between axons, including both pioneer-follower and community effects. We conclude that axon-axon interactions and ligand-receptor signaling have co-equal roles, cooperating to ensure the fidelity of axon guidance in developing vertebrate tracts.
The language of change? Characterizations of in‐group social position, threat, and the deployment of distinctive group attributes
Authors: Livingstone Andrew G. , Spears Russell , Manstead Antony S. R.
Published: July 2008

A considerable body of research has shown that group members establish and emphasize characteristics or attributes that define their in‐group in relation to comparison out‐groups. We extend this research by exploring the range of ways in which members of the same social category (Welsh people) deploy a particular attribute (the Welsh language) as a flexible identity management resource. Through a thematic analysis of data from interviews and two public speeches, we examine how the deployment of the Welsh language is bound up with characterizations of the in‐group's wider intergroup position (in terms of power relations and their legitimacy and stability), and one's position within the in‐group. We focus in particular on the rhetorical and strategic value of such characterizations for policing in‐group boundaries on the one hand, and for the in‐group's intergroup position on the other. We conclude by emphasizing the need to (1) locate analyses of the uses and importance of group‐defining attributes within the social setting that gives them meaning and (2) to appreciate such characterizations as attempts to influence, rather than simply reflect that setting.
Two routes to perspective: Simulation and rule‐use as approaches to mentalizing
Authors: Mitchell Peter , Currie Gregory , Ziegler Fenja
Published: July 2008

We review evidence relating to children's ability to acknowledge false beliefs within a simulation account according to which our focus is set by default to the world as we know it: hence, our current beliefs assume salience over beliefs that do not fall into this category. The model proposes that the ease with which we imaginatively shift from this default depends on the salience of our current belief, relative to the salience of the belief that is being simulated. However, children do use a rule‐based approach for mentalizing in some contexts, which has the advantage of protecting them from the salience of their own belief. Rule‐based mentalizing judgements might be faster, cognitively easier and less prone to error, relative to simulation‐based judgements that are much influenced by salience. We propose that although simulation is primary, rule‐based approaches develop as a shortcut; we thus grow from individuals capable of using only simulation into individuals capable of both techniques.
The implementation of the NICE guidelines for schizophrenia: Barriers to the implementation of psychological interventions and recommendations for the future
Authors: Berry Katherine. , Haddock Gillian.
Published: July 2008

Purpose. ‘Schizophrenia: Core Interventions in the Treatment and Management of Schizophrenia in Primary and Secondary Care’ was the first National Institute of Clinical Effectiveness (NICE) guidance to be produced in 2002. This guidance includes a recommendation in relation to cognitive behavioural therapy (CBT) and family interventions (FI) in schizophrenia. This review reviews this guidance and assesses the extent of their implementation in services and the barriers to this implementation.

Methods. The review draws on data from empirical studies specifically focused on the NICE guidance for FI for schizophrenia, papers discussing implementation issues in relation to other psychological therapies in schizophrenia and empirical studies investigating the implementation of similar evidence‐based guidelines in Australia and the USA.

Results. Although there is relatively limited research investigating implementation of the NICE guidelines, the available evidence suggests that people with schizophrenia still have poor access to psychological interventions. Barriers to implementation involve factors associated with: mental health professionals, the needs of service recipients and of organizations.

Conclusions. In order to improve the implementation of the updated version of the schizophrenia guidance due to be published in 2009, it will therefore be necessary to target barriers at all of these levels. There are also problems associated with the way in which the NICE guidance, generally, and those for psychological interventions in schizophrenia in particular, are developed. It will be necessary to address these in order to facilitate successful implementation.
Monodisperse single nanodiamond particulates
Authors: Ōsawa Eiji
Published: July 2008

Detonation nanodiamond (DN) was discovered in 1963, but for several reasons was known only among a small number of scientists until the turn of the century. The most serious cause was the fact that primary nanocarbon particles formed by the "bottom-up method" are in general covalently bound together under high-temperature and -pressure conditions to form large agglutinates, which were difficult to separate by conventional methods. DN was not an exception. A breakthrough led to the isolation of primary particles having the expected size of 4-5 nm by wet-milling with zirconia micro-beads. Thus, long-waited primary particles of DN finally became available in kg quantities in the form of colloidal sol, gel, and readily redispersible flakes. Progress in the development of a new form of the old material is presented.
Design of green microorganized systems for decontamination of ecotoxicants
Authors: Popov Anatoliy F.
Published: July 2008

Due to population upsurge, pesticides (derivatives of organophosphorus acids included) find widespread use in agriculture. The toxicity and long-term environmental hazard of such compounds require detailed studies on decomposition mechanisms of the pesticides and development of efficient, readily available, and inexpensive systems for their decontamination. A simple and straightforward method for the decomposition of organophosphorus compounds involves their reactions with nucleophiles. Studies on the reactivity of "normal" and α-nucleophiles toward electron-deficient centers allowed us to state a number of basic principles on the nature of the α-effect. One of the most important conclusions is: It is unlikely that the structure variations in the known α-nucleophiles will result in higher reactivity than that of hydroxylamine anion. As a practical matter, the essential disadvantages of decomposition of organophosphorus compounds in water and organic solvents are: (i) instability of active nucleophiles and oxidizing agents, (ii) corrosiveness, and (iii) extremely low solubility of organophosphorus compounds in water. These dictate the strategy of further studies, namely, carrying out the reaction in microorganized media. The first research line involves the development of the functional imidazole-based detergents functionalized with α-nucleophilic fragments. A number of efficient detergents were first synthesized. An examination of the nucleophilicity of the functional fragments in water and in the micellar phase and quantitative assessment of the factors responsible for the micellar effects showed unambiguously that the main cause of the observed accelerations is the substrate concentrating in the micellar pseudophase. The second research line consists of studies on the reactivity of versatile oxidative/nucleophilic systems involving H2O2/activator and polyhalide ion organocomplexes in water, aqueous alcohols, and micelles of cationic detergents. The novel sources of "active" halogen are highly competitive both with conventional hazardous chlorine derivatives and "green" systems involving H2O2/activator.
Synthesis and applications of functionalized silsesquioxane polymers attached to organic and inorganic matrices
Authors: Gushikem Yoshitaka , Benvenutti Edilson V. , Kholin Yuriy V.
Published: July 2008

Organofunctionalized silsesquioxane polymers obtained in a water-soluble form can be used to coat various substrates such as SiO2, SiO2/Al2O3, Al2O3, cellulose/Al2O3, and graphite or, when obtained in a water-insoluble form, can be used directly. These organofunctionalized silsesquioxanes can also be attached to poly(dimethylsiloxane) (PDMS) polymers. The functional groups constituted by neutral amine groups or cationic groups (pyridinium, 3- and 4-picolinium, or 1,4-diazabicyclo[2.2.2]octane (DABCO), mono- or -dicationic) have relatively high affinity for metal ion in ethanol solutions, as shown by their stability constants. Materials containing attached cationic functional groups have also been efficiently used to immobilize various electroactive species and to construct electrochemical sensors for analytical applications. This work discusses the preparation of silsesquioxane derivatives, their characterization as prepared and when dispersed on several substrates, and comments on some applications of these materials, with an emphasis on the metal adsorption process and manufacture of electrochemical sensors.
Some Thoughts About Reasoning in Clinical Neuropsychology
Authors: Siegert Richard J.
Published: July 2008

The present paper argues that discussion of the role of reasoning in clinical neuropsychology has been largely restricted to a debate over the reliability and validity of end-stage decision-making. This has sometimes led to heated debate, but has not resulted in any careful consideration of either the process of clinical reasoning or the cognition of the clinician. There is already a wealth of theory and research on the kinds of errors typical of human judgement and decision-making. Moreover, much of this work is particularly relevant for neuropsychology, being frequently based on research on medical diagnosis. This literature is briefly reviewed, with examples that demonstrate the relevance of research in this area for clinical neuropsychology. Then, a step-by-step approach is taken to examining the process of clinical neuropsychological assessment, with consideration at each step of some of the issues that arise demanding clinical reasoning. Finally, the article is briefly summarised and some implications for clinical training are advanced.
Method, Judgement, and Clinical Reasoning
Authors: Ward Tony
Published: July 2008

Researchers have tended to take one of two mutually exclusive positions concerning the nature and status of clinical decision-making. On the one hand, clinicians are urged to be more rigorous and analytical when assessing a client, to disregard their intuitions and instead utilise explicit rules and algorithms. On the other hand, they are counselled to regard their “gut feelings” as valuable sources of knowledge about clients. As a way of reconciling these two perspectives, it is important to acknowledge that clinical psychologists are confronted with a wide range of assessment and clinical tasks that vary in their degree of structure. Therefore, in order to effectively manage the diverse tasks they face during a typical assessment, they need to possess a wide range of cognitive skills. These skills, and their associated cognitive tasks, will span the cognitive continuum from the intuitive to the analytical poles (Hammond, 1996).
Effects of Images on the Renewal of Blood-Injury Fear
Authors: Page Andrew C.
Published: July 2008

The ability of images to bring about a renewal of fear was examined. Thirty-seven psychology students were repeatedly exposed to a slide depicting an injured face. The experimental subjects listened to a narrative that elicited images of fear while the control subjects listened to a narrative that elicited images of anger for three of the exposure trials. On the fourth and final trial, half of the subjects from each condition listened to a narrative that elicited different images, while the remainder continued to listen to the same narrative. There was no effect of narrative content on fear, but changing the narrative (either from fear to anger or vice versa) led to an increase in fear. These data suggest that the act of changing images may lead to a renewal of fear.
The Diagnostic Interview Schedule for Children, Adolescents, and Parents: Initial Reliability and Validity Data
Authors: Johnson Sally , Barrett Paula M. , Dadds Mark R. , Fox Tara , Shortt Alison
Published: July 2008

This study evaluated the psychometric properties of the Diagnostic Interview Schedule for Children, Adolescents and Parents (DISCAP; Holland & Dadds, 1995), for DSM-IV anxiety disorders in children and adolescents. Two studies were conducted to examine the reliability and validity of the DISCAP. In the first study, the DISCAP and the Youth Self Report (YSR; Achenbach, 1991c) were administered to 120 nonclinical adolescents aged 12 through to 14 years. In the second study, the DISCAP and Child Behaviour Checklist (CBCL; Achenbach, 1991b) were administered to parents of 57 clinical children and adolescents aged 6 through to 16 years. Inter-rater reliability data was collected, and both concurrent and discriminant validity of the DISCAP were assessed against the YSR and CBCL. Inter-rater agreements for primary diagnoses were high, and rating scale data supported the concurrent and discriminant validity of the DISCAP diagnoses. Results suggest that the DISCAP can be used to facilitate reliable and valid diagnoses of childhood anxiety disorders.
Relapse Prevention: A Model for Psychosis?
Authors: Beck-Sander Alison
Published: July 2008

Evidence suggests that there are substantial costs (in a variety of spheres: personal, social, financial, etc.) associated with psychotic relapse. Furthermore, relapse increases the likelihood of future relapses and increased costs. This paper explores the application of a model of relapse prevention (RP) outlined by Marlatt and Gordon (1985) to the field of psychosis. The original model was designed to help individuals who abuse substances to maintain control over their habit. The advantages and limitations of applying the original model to the prevention of psychotic relapse are explored in detail in the context of an amended model. One major advantage of the RP model is that it provides a framework for the coordination of treatment interventions. The complex intervention packages, which may be useful in the management of psychotic illnesses, are described. The RP framework may be used to coordinate treatments in a manner that maximises their potential and emphasises patient control.
Inferential Statistics: Criticised by Sidman (1960), but Popular in the Journal of the Experimental Analysis of Behavior
Authors: Foster T. Mary , Jarema Kimberly , Poling Alan
Published: July 2008

There are many criticisms of the use of inferential statistics in behavioural research, including those made by Sidman (1960). This paper reports the frequency of use of inferential statistics in the Journal of the Experimental Analysis of Behavior in every fifth year from 1960. The usage is apparently growing. It is argued that is it time to return to appropriate experimental control.
Stress Management for Atopic Dermatitis
Authors: Habib Suzanne , Morrissey Shirley
Published: July 2008

Atopic dermatitis (AD) is a chronic, debilitating skin disorder that accounts for up to 20% of dermatological diagnoses. A 6-week psychoeducational stress management program was developed, implemented, and evaluated as an adjunctive treatment for AD. The participants (n= 17) were randomly assigned to a treatment or waiting-list control group. Participants in both the intervention and waiting-list control groups were assessed for dermatitis severity by a blind rater both pre and posttreatment and at a follow-up conducted 8 weeks after the conclusion of the program. At posttest the intervention group had significantly reduced pruritus and global severity of atopic dermatitis, and reduced levels of social anxiety and private self-consciousness. At an 8-week follow-up, pruritus was entirely absent and global severity was continuing to decrease, as were levels of social anxiety and private self-consciousness. The psychoeducational stress-management program provided a short effective treatment that resulted in reduction of symptoms and provided long-term management strategies to sufferers of atopic dermatitis.
Worry and Defensive Pessimism: A Test of Two Intervention Strategies
Authors: Thompson Ted , Mason Brontë , Montgomery Iain
Published: July 2008

This study assessed the effectiveness of counterfactual reasoning and relaxation training in reducing worry and defensive pessimism. Thirty-three participants classified as both worriers and defensive pessimists were allocated to either counterfactual reasoning, relaxation training, or control groups. Participants in counterfactual reasoning and relaxation training groups attended four weekly training sessions. Significant reductions in worry between pre- and post-test were evident for scores on the Worry Domains Questionnaire, as well as a reduced tendency to engage in the kind of prototypical thinking characteristic of defensive pessimists as assessed by the Defensive Pessimism / Strategic Optimism Strategy Prototypes Questionnaire. Reductions were also evident in the percentage of the day spent worrying, the intrusiveness of worries, and ratings of the likelihood of worry events occurring.
Doctors Are More Dangerous Than Gun Owners: A Rejoinder to Error Counting
Authors: Dekker Sidney W. A.
Published: July 2008

Objective: This paper analyzes some of the problems with error counting as well as the difficulty of proposing viable alternatives. Background: Counting and tabulating negatives (e.g., errors) are currently popular ways to measure and help improve safety in a variety of domains. They uphold an illusion of rationality and control but may offer neither real insight nor productive routes for improving safety. Method: The paper conducts a critical analysis of assumptions underlying error counting in human factors. Results: Error counting is a form of structural analysis that focuses on (supposed) causes and consequences; it defines risk and safety instrumentally in terms of minimizing negatives and their measurable effects. In this way, physicians can be proven to be 7500 times less safe than gun owners, as they are responsible for many more accidental deaths. Conclusion: The appeal of error counting may lie in a naive realism that can enchant researchers and practitioners alike. Supporting facts will continue to be found by those looking for errors through increasingly refined methods. Application: The paper outlines a different approach to understanding safety in complex systems that is more socially and politically oriented and that places emphasis on interpretation and social construction rather than on putatively objective structural features.
A Methodology for Identifying Human Error in U.S. Navy Diving Accidents
Authors: O'Connor Paul , O'Dea Angela , Melton John
Published: July 2008

Objective: To better understand how human error contributes to U.S. Navy diving accidents. Background: An analysis of 263 U.S. Navy diving accident and mishap reports revealed that the human factors classifications were not informative for further analysis, and 70% of mishaps were attributed to unknown causes; only 23% were attributed to human factors. Method: Five diving fatality reports were examined using the consensual qualitative research (CQR) method to develop a taxonomy of six categories and 21 subcategories for classifying human errors in diving. In addition, 15 critical incident technique (CIT) interviews were conducted with U.S. Navy divers who had been involved in a diving accident or near miss and analyzed using the dive team error taxonomy. Results: Overall, failures in situation awareness and leadership were the most common human errors made by the dive team. Conclusion: The dive team human error taxonomy could aid in accident investigation and in the training and evaluation of U.S. Navy divers. Application: The development of the dive team human error taxonomy has generated a number of considerations that researchers should take into account when developing, or adapting, an error taxonomy from one industry to another.
A Review of Medical Error Reporting System Design Considerations and a Proposed Cross-Level Systems Research Framework
Authors: Holden Richard J. , Karsh Ben-Tzion
Published: July 2008

Objective: To review the literature on medical error reporting systems, identify gaps in the literature, and present an integrative cross-level systems model of reporting to address the gaps and to serve as a framework for understanding and guiding reporting system design and research. Background: Medical errors are thought to be a leading cause of death among adults in the United States. However, no review exists summarizing what is known about the barriers and facilitators for successful reporting systems, and no integrated model exists to guide further research into and development of medical error reporting systems. Method: Relevant literature was identified using online databases; references in relevant articles were searched for additional relevant articles. Results: The literature review identified components of medical error reporting systems, error reporting system design choices, barriers and incentives for reporting, and suggestions for successful reporting system design. Little theory was found to guide the published research. An integrative cross-level model of medical error reporting system design was developed and is proposed as a framework for understanding the medical error reporting literature, addressing existing limitations, and guiding future design and research. Conclusion: The medical error reporting research provides some guidance for designing and implementing successful reporting systems. The proposed cross-level systems model provides a way to understand this existing research. However, additional research is needed on reporting and related safety actions. The proposed model provides a framework for such future research. Application: This work can be used to guide the design, implementation, and study of medical error reporting systems.
What You Don't Know Can Hurt You: Factors Impacting Diagnosis in the Automated Cockpit
Authors: Mosier Kathleen L. , Sethi Nikita , McCauley Shane , Khoo Len , Orasanu Judith M.
Published: July 2008

Objective: We examined the impact of operational variables on diagnosis and decision-making processes, focusing on information search. Background: Arguably, the “best” decision-making processes in high-technology cockpits would be those that are both correspondent (objectively accurate) and coherent (rationally sound). In the electronic world, coherence in terms of identification and incorporation of all relevant information is both a prerequisite to and a limiting factor for accurate diagnosis and decision making. Method: Regional carrier pilots ( N = 93) responded to six scenarios by accessing information to determine a diagnosis and decision. Results: Time pressure, a common operational variable, had a strong negative effect on information search and diagnosis accuracy, and the presence of noncongruent information heightened these negative effects. Unexpectedly, source of initial information (automated or other) did not impact any of the dependent variables. Diagnosis confidence was unrelated to accuracy and was negatively related to amount of information accessed. Conclusion: Results confirm both the need for coherence in diagnostic processes and the difficulty of maintaining it under time pressure. Application: One implication of the results of this study is that pilots in high-technology cockpits must be trained to utilize coherent diagnostic processes as standard operating procedure. Additionally, because thorough information search for diagnosis in an automated environment is essential, automated systems must be designed to foster coherent, and thus accurate, diagnostic processes.
Intelligent Transportation Systems – Opportunities and Challenges (Intelligente Verkehrssysteme – Neue Möglichkeiten und Fragestellungen)
Authors: Busch Fritz
Published: July 2008

Transportation systems currently undergo a significant alteration. Social changes and increased expectations towards protection of environment and resources, safety and efficiency are faced by new opportunities of information and communication technology. The emerging opportunities raise new questions to traffic behaviour, liabilities, market introduction and others.