bluuebunny/crossref_metadata_2025 huggingface dataset:
Lettuce Contamination and Survival of Salmonella Typhimurium and Listeria monocytogenes in Hydroponic Nutrient Film Technique Systems
Authors: Ilic Sanja , Moodispaw Margaret R. , Madden Lawrence V. , Lewis Ivey Melanie L.
Published: November 2022

Hydroponic vegetable production is increasing globally, but there is a lack of science-based recommendations to ensure their food safety. Specifically, there is limited evidence for establishing water management strategies. The purpose of this study was to determine the survival of Salmonella Typhimurium and Listeria monocytogenes in commercial nutrient flow technology (NFT) systems during the lifecycle of lettuce exposed to sporadic or extreme contamination. NFT systems were inoculated with Salmonella Typhimurium or Listeria monocytogenes, and nutrient solution, rockwool, roots, and lettuce leaves were collected over the lettuce production cycle for pathogen enumeration and detection. Both human pathogens persisted in the lettuce NFT growing system throughout the growth cycle of lettuce. Salmonella Typhimurium and L. monocytogenes accumulated in rockwool medium and on lettuce roots and were transferred to the leaves at quantifiable levels from the contaminated nutrient solution. In the nutrient solution, Salmonella concentration under sporadic and extreme conditions declined significantly 24 h after inoculation and again 7 days post-inoculation (p < 0.0001). Under extreme conditions, the concentration did not change significantly after 7 days, while under sporadic conditions, the concentration declined again 14 days post-inoculation in the nutrient solution collected from the reservoirs. L. monocytogenes populations in the nutrient solution fluctuated significantly over the 28-day growth cycle (p < 0.0001). Under extreme conditions, L. monocytogenes concentrations in the nutrient solution declined, while under sporadic conditions, the populations increased. The findings of this study, for the first time, describe human pathogen survival in commerical NFT systems and highlight the urgent need for novel approaches to mitigating the risks from nutrient solution contaminaiton in hydroponics.
Design of a Greenhouse Solar-Assisted Heat Pump Dryer for Kelp (Laminaria japonica): System Performance and Drying Kinetics
Authors: Kang Huanyu , Zhang Guochen , Mu Gang , Zhao Cheng , Huang Haolin , Kang Chengxiang , Li Xiuchen , Zhang Qian
Published: November 2022

In order to solve a series of problems with kelp drying including long drying time, high energy consumption, low drying efficiency, and poor quality of dried kelp, this work proposes the design of a novel greenhouse double-evaporator solar-assisted heat pump drying system. Experiments on kelp solar-assisted heat pump drying (S−HP) and heat pump drying (HP) under the condition of irradiance of 100−700 W/m2 and a temperature of 30, 40, or 50 °C were conducted and their results were compared in terms of system performance, drying kinetics, and quality impact. The drying time was reduced with increasing irradiance or temperature. The coefficient of performance (COP) and specific moisture extraction rate (SMER) of S−HP were 3.590−6.810, and 1.660−3.725 kg/kW·h, respectively, roughly double those of HP when the temperatures are identical. The Deff of S-HP and HP were 5.431 × 10−11~11.316 × 10−11 m2/s, and 1.037 × 10−11~1.432 × 10−11 m2/s, respectively; additionally, solar radiation greatly improves Deff. The Page model almost perfectly described the changes in the moisture ratio of kelp by S−HP and HP with an inaccuracy of less than 5%. When the temperature was 40 °C and the irradiance was above 400 W/m2, the drying time of S−HP was only 3 h, and the dried kelp maintained the green color with a strong flavor and richness in mannitol. Meanwhile, the coefficient of performance was 6.810, the specific moisture extraction rate was 3.725 kg/kWh, and the energy consumption was 45.2%, lower than that of HP. It can be concluded that S−HP is highly efficient and energy-saving for macroalgae drying and can serve as an alternate technique for the drying of other aquatic products.
Effect of Heat–Moisture Treatment on the Physicochemical Properties, Structure, Morphology, and Starch Digestibility of Highland Barley (Hordeum vulgare L. var. nudum Hook. f) Flour
Authors: Lv Yiming , Ma Sen , Yan Jingyao , Sun Binghua , Wang Xiaoxi
Published: November 2022

This study modified native highland barley (HB) flour by heat–moisture treatment (HMT) at different temperatures (90, 110, and 130 °C) and moisture contents (15%, 25%, and 35%). The effects of the treatment on the pasting, thermal, rheological, structural, and morphological properties of the native and HMT HB flour were evaluated. The results showed that HMT at 90 °C and 25% moisture content induced the highest pasting viscosity (3626–5147 cPa) and final viscosity (3734–5384 cPa). In all conditions HMT increased gelatinization temperature (To, 55.77–73.72 °C; Tp, 60.47–80.69 °C; Tc, 66.16–91.71 °C) but decreased gelatinization enthalpy (6.41–0.43 J/g) in the HMT HB flour compared with that in the native HB flour. The HB flour treated at 15% moisture content had a higher storage modulus and loss modulus than native HB flour, indicating that HMT (moisture content, 15%, 25%, and 35%) favored the strengthening of the HB flour gels. X-ray diffraction and Fourier-transform infrared spectroscopy results showed that HMT HB flour retained the characteristics of an A-type crystal structure with an increased orderly structure of starch, while the relative crystallinity could be increased from 28.52% to 41.32%. The aggregation of starch granules and the denaturation of proteins were observed after HMT, with additional breakage of the starch granule surface as the moisture content increased. HMT could increase the resistant starch content from 24.77% to 33.40%, but it also led to an increase in the rapidly digestible starch content to 85.30% with the increase in moisture content and heating temperature. These results might promote the application of HMT technology in modifying HB flour.
Grain Quality Characterization of Hybrid Rice Restorer Lines with Resilience to Suboptimal Temperatures during Filling Stage
Authors: Lu Xuedan , Wang Lu , Xiao Yunhua , Wang Feng , Zhang Guilian , Tang Wenbang , Deng Huabing
Published: November 2022

Rice (Oryza sativa L.) is a staple food that is consumed worldwide, and hybrid rice has been widely employed in many countries to greatly increase yield. However, the frequency of extreme temperature events is increasing, presenting a serious challenge to rice grain quality. Improving hybrid rice grain quality has become crucial for ensuring consumer acceptance. This study compared the differences in milling quality, appearance quality, and physical and chemical starch properties of rice grains of five restorer lines (the male parent of hybrid rice) when they encountered naturally unfavorable temperatures during the filling period under field conditions. High temperatures (HTs) and low temperatures (LTs) had opposite effects on grain quality, and the effect was correlated with rice variety. Notably, R751, R313, and Yuewangsimiao (YWSM) were shown to be superior restorer lines with good resistance to both HT and LT according to traits such as head rice rate, chalkiness degree, chalky rice rate, amylose content, alkali spreading value, and pasting properties. However, Huazhan and 8XR274 were susceptible to sub-optimal temperatures at the grain-filling stage. Breeding hybrid rice with adverse-temperature-tolerant restorer lines can not only ensure high yield via heterosis but also produce superior grain quality. This could ensure the quantity and taste of rice as a staple food in the future, when extreme temperatures will occur increasingly frequently.
Sharp Bounds of Hankel Determinant on Logarithmic Coefficients for Functions Starlike with Exponential Function
Authors: Shi Lei , Arif Muhammad , Iqbal Javed , Ullah Khalil , Ghufran Syed Muhammad
Published: November 2022

Using the Lebedev–Milin inequalities, bounds on the logarithmic coefficients of an analytic function can be transferred to estimates on coefficients of the function itself and related functions. From this fact, the study of logarithmic-related problems of a certain subclass of univalent functions has attracted much attention in recent years. In our present investigation, a subclass of starlike functions Se* connected with the exponential mapping was considered. The main purpose of this article is to obtain the sharp estimates of the second Hankel determinant with the logarithmic coefficient as entry for this class.
Blazars at Very High Energies: Emission Modelling
Authors: Sol Hélène , Zech Andreas
Published: November 2022

Blazars are very broadband cosmic sources with spectra spanning over twenty orders of magnitude in frequency, down to the 100 MHz regime in the radio range, up to VHE at several tens of TeV. The modelling of their spectral energy distribution at high energies currently considers two main classes of models, leptonic and lepto-hadronic, which both succeed fairly well in describing the observed spectra for the two populations of blazars, namely BL Lac objects (BL Lacs) and flat spectrum radio quasars (FSRQs). However they are both confronted with difficulties, in particular to reproduce flaring phenomena monitored with a good multi-spectral and temporal coverage, or to reproduce extreme sources which challenge the basic descriptions. Such a situation has led to a diversity of specific scenarios, the positioning of which in relation to the general context of the sources is generally not clearly fixed. The identification of the dominant particle acceleration mechanism at work and a better understanding of the location of the TeV emitting zone would make it possible to break the degeneracies between models. Multi-wavelength and multi-messenger studies should also help in this regard, with the perspective to elaborate a general reference scenario of blazars and AGNs.
Mechanistic Insights into the Selective Synthesis of 4H-Pyran Derivatives On-Water Using Naturally Occurring Alginate from Sargassum muticum: Experimental and DFT Study
Authors: Oudghiri Khaoula , Belattmania Zahira , Elmouli Hamid , Guesmi Salaheddine , Bentiss Fouad , Sabour Brahim , Bahsis Lahoucine , Taourirte Moha
Published: November 2022

The naturally occurring sodium alginate (SA) biopolymer from the Sargassum muticum (Yendo) Fensholt was employed as a green organocatalyst for the synthesis of 4H-pyran derivatives. The naturally extracted macromolecule was fully characterized using different analyses, including nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and Energy Dispersive X-ray Analysis (EDX). The catalytic activity of SA was investigated in the one-pot reaction between aldehydes, malononitrile, and 1,3-dicarbonyl compounds in water at room temperature, and the corresponding 2-amino-3-cyano-4H-pyran derivatives were obtained with good to excellent yields. This organocatalyst was easily separated from the reaction mixture and reused for at least two consecutive cycles without a significant loss of its catalytic activity or selectivity. From the mechanistic point of view, density functional theory (DFT) and NCI analyses were performed for the first time to explain the regioselectivity outcomes for the synthesis of 2-amino-3-cyano-4H-pyran derivatives using SA as a green organocatalyst.
Different Effects of Perceived Social Support on the Relationship between Perceived Stress and Depression among University Students with Borderline Personality Disorder Symptoms: A Multigroup Mediation Analysis
Authors: Ingkachotivanich Narusorn , Wongpakaran Tinakon , Wongpakaran Nahathai , Oon-Arom Awirut , Karawekpanyawong Nuntaporn , Lohanan Trustsavin , Leesawat Thanakorn
Published: November 2022

Background: While perceived social support can mediate the relationship between perceived stress and depression, little is known about the differences between individuals with high and low borderline personality disorder symptoms (BPDS). This study aimed to investigate the associations among perceived stress, perceived social support, and depression, and compare low and high levels of BPDS. Methods. This cross-sectional analysis was a secondary analysis of data from the SI-Bord study. University students across Thailand completed a screening instrument for borderline personality disorder, the Perceived Stress Scale (PSS), the Revised Thai Multi-dimensional Scales of Perceived Social Support (MSPSS), and the Patient-Health Questionnaire (PHQ)-9. Mediation analysis using PROCESS was applied to test the direct and indirect effects of perceived stress on depression. Multigroup mediational analysis was adopted to compare low and high levels of BPDS. Results. The mean age of the 330 participants was 20.27 (SD, 1.4) and 80% were female. Significant correlations were observed between the PSS, MSPSS, and PHQ scores, with greater magnitude among the high-level BPDS group (p < 0.001). A significant direct effect on perceived stress and a significant indirect effect on depression through perceived social support were noted. Of all the sources of social support, only the significant others variable significantly differed between the two groups (p < 0.05). Conclusion. Perception of social support had a significant mediating role in perceived stress and depression. The magnitude of associations was remarkably high for individuals with high BPDS compared to those with low BPDS. Unlike those with low BPDS, all sources of social support were significant mediators between the two groups.
Direct Evidence of Metal Type Printing in The Song of Enlightenment, Korea, 1239
Authors: Yoo Woo Sik
Published: November 2022

Since the 1920s, nearly six identical versions of an ancient printed book, The Song of Enlightenment with Commentaries by Buddhist Monk Nammyeong Cheon (南明泉和尙頌證道歌), have been found in Korea. Until very recently, they were believed to be woodblock-printed versions from the 13th to 16th centuries using woodblocks carved from the sheets of a metal-type-printed version from 1239. Two of the six versions were once identified to be woodblock prints in the Goryeo dynasty in the 13th century and designated as Korean treasures in 1984 and 2012. In 2021, one woodblock-printed version printed during the Joseon dynasty in 1526, was designated as a treasure of the Metropolitan city of Seoul, Korea. Historians in Korea have been in heated debate over the printing techniques (metal type print for one version or all woodblock prints) and printing dates (or sequence) of the two versions designated as Korean treasures for the last 50 years. It was almost a never-ending debate with struggles and anger among Korean historians due to the very subjective nature of the examination method and decision-making process by consensus. The heated debates in Korea were never brought to the world’s attention, outside of Korea, and are still considered to be a taboo subject in Korea. To conclude this heated debate with direct evidence of metal type printing of the particular version of interests, all six versions were examined by image comparisons and quantitative analyses of inked areas of individual characters, lines of characters, pages and borderlines. All claims against the possibility of metal type printing of the particular version were reviewed thoroughly. Very clear circumstantial and physical evidence for metal type printing of the version designated as a Korean treasure in 2012 was found. The version carries more than metal casting defects and has the smallest inked area (characters with thin strokes) among all six versions. The version of interest was very likely printed using movable metal type in September 1239, as indicated in the imprint, and is definitely the world’s oldest extant book, printed using metal type in Korea in 1239, predating Jikji (1377) by 138 years and the 42-line Gutenberg Bible (1455) by 216 years.
Volatile Profiles of Vidal Grapes in the Shangri-La High-Altitude Region during On-Vine Non-Destructive Dehydration
Authors: Xu Qing-Fang , Fan Liang , Lu Kai-Xiang , Zhao Dong-Mei , Zhang Ming-Xia , Cai Jian
Published: November 2022

Both free and glycosidically bound forms of volatile compounds in Vidal grapes from the Shangri-La high-altitude region during the on-vine non-destructive dehydration process were investigated by headspace solid-phase micro-extraction (HS-SPME) combined with gas chromatography–mass spectrometry (GC-MS), following which the data were processed by multivariate statistical analysis. Fatty-acid-derived volatiles (FADs), amino-acid-derived volatiles (AADs), and isoprene-derived volatiles (IPDs), which occurred mainly in bound forms, were the three major volatiles in dehydrated Vidal grapes. Water-loss concentration, biosynthesis, and biodegradation all occurred during dehydration, eventually modifying some volatiles significantly, especially some powerful odorants such as hexanal, trans-2-hexenal, 2-phenethyl acetate, β-myrcene, linalool, geraniol, cis-rose oxide, and β-damascenone. 1-Octen-3-ol was relatively stable during the non-destructive on-vine dehydration process and its content in grape juice was mainly determined by the concentration effect. 2,4-Di-tert-butylphenol, 2-phenethyl acetate, 2-methyl-1-propanol, and hexanol were screened as some of the most important metabolic markers to discriminate grapes at different dehydration degrees. Our study also highlights the fundamental importance of the expression of volatile content in the metabolomic study of grape berries.
Study of Primary and Secondary Metabolites of Stenospermocarpic, Parthenocarpic and Seeded Raisin Varieties
Authors: Kaya Ozkan , Ates Fadime , Kara Zeki , Turan Metin , Gutiérrez-Gamboa Gastón
Published: November 2022

(1) Background: Stenospermocarpic (Sultani Çekirdeksiz and Black Kishmish), parthenocarpic (Black Corinth), and seeded varieties (Ekşi Kara and Gök Üzüm) are used for raisin production. To our knowledge, there is little available information about the biochemical characteristics of raisins produced from these varieties. (2) Methods: Some metabolites, such as hormones, sugars, vitamins, minerals, and amino acids, including enzymatic activity, were determined in different raisin varieties. (3) Results: Seedless raisin varieties presented higher content of several hormones, vitamins, and minerals, as antioxidant capacity than the raisins produced from seeded varieties. Contrary to this, seeded raisin varieties presented higher contents of most measured sugars and amino acids than the raisins produced from seedless varieties. (4) Conclusions: Biological mechanisms of pollination and fertilization induced modifications in the primary and secondary metabolism of grapes, considerably affecting biochemical compounds and the antioxidant capacity of raisins.
The Impact of the Sweetened Beverages Tax on Their Reformulation in Poland—The Analysis of the Composition of Commercially Available Beverages before and after the Introduction of the Tax (2020 vs. 2021)
Authors: Wierzejska Regina Ewa
Published: November 2022

The aim of this study was to estimate changes in the composition of carbonated and non-carbonated sugar-sweetened beverages before and after the introduction of the beverage tax in Poland. Based on the labels of 198 drinks, the composition and nutritional values of the drinks were compared. The nonparametric Mann–Whitney test was applied to compare the differences in the sugar and juice content as well as energy value. After the introduction of the tax, the median sugar content in the carbonated beverages decreased from 8.6 g to 6.9 g/100 mL (p = 0.004), while in the non-carbonated beverages, it decreased from 5.5 g to 4.8 g/100 mL (p < 0.001). In the entire beverage group, there was a significant drop in the proportion of beverages that contained >5 g of sugars/100 mL (44.4% in 2021 vs. 70.2% in 2020). The median juice content in the carbonated beverages increased from 1.0% to 3.3% (p = 0.121), but totalled 20.0% for both periods in the non-carbonated beverages. The percentage of beverages with a tax-exempt composition (juice content ≥ 20% and sugar content ≤ 5 g/100 mL) almost tripled. After the introduction of the tax, beneficial changes in the compositions of 62% of the analysed beverages were observed in terms of their sugar and/or juice content.
Minocycline Attenuates Lipopolysaccharide-Induced Locomotor Deficit and Anxiety-like Behavior and Related Expression of the BDNF/CREB Protein in the Rat Medial Prefrontal Cortex (mPFC)
Authors: Abdo Qaid Entesar Yaseen , Abdullah Zuraidah , Zakaria Rahimah , Long Idris
Published: November 2022

Neuroinflammation following lipopolysaccharide (LPS) administration induces locomotor deficits and anxiety-like behaviour. In this study, minocycline was compared to memantine, an NMDA receptor antagonist, for its effects on LPS-induced locomotor deficits and anxiety-like behaviour in rats. Adult male Sprague Dawley rats were administered either two different doses of minocycline (25 or 50 mg/kg/day, i.p.) or 10 mg/kg/day of memantine (i.p.) for 14 days four days prior to an LPS (5 mg/kg, i.p.) injection. Locomotor activity and anxiety-like behaviour were assessed using the open-field test (OFT). The phosphorylated tau protein level was measured using ELISA, while the expression and density of brain-derived neurotrophic factor (BDNF) and cAMP response element-binding (CREB) protein in the medial prefrontal cortex (mPFC) were measured using immunohistochemistry and Western blot, respectively. Minocycline treatment reduced locomotor deficits and anxiety-like behaviour associated with reduced phosphorylated tau protein levels, but it upregulated BDNF/CREB protein expressions in the mPFC in a comparable manner to memantine, with a higher dose of minocycline having better benefits. Minocycline treatment attenuated LPS-induced locomotor deficits and anxiety-like behaviour in rats and decreased phosphorylated tau protein levels, but it increased the expressions of the BDNF/CREB proteins in the mPFC.
The Importance of Charge Transfer and Solvent Screening in the Interactions of Backbones and Functional Groups in Amino Acid Residues and Nucleotides
Authors: Sladek Vladimir , Fedorov Dmitri G.
Published: November 2022

Quantum mechanical (QM) calculations at the level of density-functional tight-binding are applied to a protein–DNA complex (PDB: 2o8b) consisting of 3763 atoms, averaging 100 snapshots from molecular dynamics simulations. A detailed comparison of QM and force field (Amber) results is presented. It is shown that, when solvent screening is taken into account, the contributions of the backbones are small, and the binding of nucleotides in the double helix is governed by the base–base interactions. On the other hand, the backbones can make a substantial contribution to the binding of amino acid residues to nucleotides and other residues. The effect of charge transfer on the interactions is also analyzed, revealing that the actual charge of nucleotides and amino acid residues can differ by as much as 6 and 8% from the formal integer charge, respectively. The effect of interactions on topological models (protein -residue networks) is elucidated.
Dual Negativity of CD56 and CD117 Links to Unfavorable Cytogenetic Abnormalities and Predicts Poor Prognosis in Multiple Myeloma
Authors: Zheng Dong , Zhu Mingxia , Li Qihui , Wan Wenli , Chen Yingtong , Jing Hongmei
Published: November 2022

The prognostic value of CD56 and CD117 expression on myeloma cells is controversial. This study aims to analyze the correlation of CD56 and CD117 expression with cytogenetic abnormalities and survival. A total of 128 patients with newly diagnosed multiple myeloma (NDMM) were recruited in this single-center retrospective study. Flow cytometry and FISH tests of marrow cells were performed for all of the subjects. The statistical methods included a chi-squared test, univariate and multivariate COX regressions, and a Kaplan-Meier survival curve analysis. Regarding the cytogenetics, the incidence of IgH/FGFR3 translocation was more frequent in patients with a negative CD56 (p = 0.003). CD56 negativity was an independent adverse factor associated with a poor prognosis (p = 0.019) and indicated a shorter overall survival (OS) (p = 0.021). Patients with dual negative CD56 and CD117 trended toward a poorer OS (CD56−CD117− vs. CD56+CD117−, p = 0.011; CD56−CD117− vs. CD56+CD117+, p = 0.013). In conclusion, CD56 is a prognostic marker that independently affects OS and is associated with adverse cytogenetic abnormalities. Patients with a dual negativity of CD56 and CD117 have a worse clinical outcome.
Stomatognathic Diseases: State of the Art and Future Perspectives
Authors: Guida Agostino , Warnakulasuriya Saman
Published: November 2022

The World Health Organization (WHO) considers oral heath to be a key indicator of overall health, as it is linked to physical well-being and quality of life [...]
High Expressed Emotion (HEE), Assessed Using the Five-Minute Speech Sample (FMSS), as a Predictor of Psychiatric Relapse in Patients with Schizophrenia and Major Depressive Disorder: A Meta-Analysis and Meta-Regression
Authors: Mazza Cristina , Formica Federico , Ferracuti Stefano , Ricci Eleonora , Colasanti Marco , Biondi Silvia , Di Domenico Alberto , Roma Paolo
Published: November 2022

Expressed Emotion (EE) describes the tone of a caregiver’s response to a patient with a mental disorder, and it is used to predict relapse. The Five-Minute Speech Sample (FMSS) is a 5-min interview with a caregiver that evaluates only two EE dimensions. The present study aimed at evaluating HEE (High Expressed Emotion) as a predictor of relapse in patients with schizophrenia and major depressive disorder. Six studies were selected for the meta-analysis. In total, the studies included 297 subjects. The analyses included a random effects model; a meta-analysis excluding the study with the smallest sample; a cumulative meta-analysis; a meta-regression with random effects, using patient age and duration of illness as moderators; a leave-one-out meta-analysis; and a funnel plot to estimate publication bias. The FMSS emerged as a valid and reliable tool for measuring EE as a predictor of relapse in patients with schizophrenia and major depressive disorder. Patient age and duration of illness had no significant effect on the results. Future meta-analyses should include more studies to reduce publication bias. EE may be a good predictor of relapse when examined through a fast measurement technique such as the FMSS, which may also be useful to analyze the psychopathological structure of caregivers.
Effect of a 6-Week Cycle of Nordic Walking Training on Vitamin 25(OH)D3, Calcium-Phosphate Metabolism and Muscle Damage in Multiple Myeloma Patients–Randomized Controlled Trial
Authors: Czerwińska-Ledwig Olga , Vesole David H. , Piotrowska Anna , Gradek Joanna , Pilch Wanda , Jurczyszyn Artur
Published: November 2022

Introduction: Multiple myeloma (MM) is a hematological malignancy affecting older adults. One of the most common myeloma-defining events is the development of symptomatic lytic bone disease. The serum concentrations of calcium (Ca), inorganic phosphorus (P), and vitamin 25(OH)D3 in the serum reflect bone metabolism. An enzyme lactate dehydrogenase (LDH) is a marker of muscle damage, but its serum activity also has an important prognostic value in MM. Myoglobin (Mb) is a small protein present in muscles; its serum level increases when myocytes are damaged. Objectives: In this study, the impact of a 6-week Nordic walking (NW) exercise program on blood parameters related to calcium-phosphate metabolism and damage of skeletal muscles was assessed. Patients and methods: A total of 33 patients with MM in the remission stage, without cytostatic treatment, were allocated and randomly assigned to one of two groups: 17 in the training group (NW) and 16 in the control group (CG). All patients were supplemented per os with vitamin D3 and calcium carbonate daily and received zoledronic acid every 4 weeks (intravenous). Nordic walking training sessions took place 3 times a week for 6 weeks, 1 h each. Blood samples were drawn before and after the 6 weeks of training sessions to assess the serum concentrations of vitamin 25(OH)D3, P, Ca, Mb, and LDH. Results: Patients from the NW group showed a statistically significant decrease in mean serum myoglobin concentration (p = 0.018) and an increase in 25(OH)D3 (p < 0.001) and total Ca (p = 0.001) concentrations. There were no statistically significant changes in the results obtained in CG. Between groups, after 6 weeks, Mb serum concentration was significantly lower in NW (p = 0.041), and 25(OH)D3 was higher (p < 0.001) compared to CG. There was a correlation between the changes in myoglobin, phosphorus, 25(OH)D3, and Ca concentrations after 6 weeks. Conclusions: NW training is a safe and beneficial form of physical exercise for patients with MM without inducing muscle damage. NW performed outside improves serum vitamin 25(OH)D3 concentration.
Cardiovascular Risk Factors before Onset of Rheumatoid Arthritis Are Associated with Cardiovascular Events after Disease Onset: A Case–Control Study
Authors: Kokkonen Heidi , Johansson Linda , Stenlund Hans , Rantapää-Dahlqvist Solbritt
Published: November 2022

Background: The increased comorbidity and mortality in rheumatoid arthritis (RA) patients are largely due to cardiovascular disease (CVD). Previously, we demonstrated increased frequencies of risk factors for CVD (elevated body mass index (BMI), elevated apoliprotein (Apo) B:ApoA1 ratio, and smoking) in pre-RA individuals compared with matched controls. Objectives: Assess the impact of traditional CV risk factors present before the onset of RA on the risk of CV events (CVE) after diagnosis in comparison with matched controls. Methods: A case–control study including 521 pre-symptomatic individuals and 1566 controls identified within the Health Surveys of the Medical Biobank was performed. CVD risk factors were hypertension, elevated ApoB:A1 ratio, BMI, diabetes, and smoking. Information on comorbidities was requested from the Swedish National Patient Register and Cause of Death Register. Results: Pre-RA individuals had a higher risk of future CVE compared with matched controls (HR [95% CI] 1.70 [1.31–2.21]), which remained after adjustments for risk factors for CVD (HR [95% CI] 1.73 [1.27–2.35]). Most risk factors were associated with CVE after diagnosis, and a combination resulted in a higher risk in RA compared with controls; two risk factors, HR [95% CI] 2.70 [1.19–6.13] vs. 1.26 [0.75–2.13]; and three to four risk factors, HR [95% CI] 6.32 [2.92–13.68] vs. 3.77 [2.34–6.00]. Conclusions: Risk factors for CVD present in pre-RA individuals were associated with future CVE, and even after adjustments for these risk factors and treatments after RA onset, pre-RA individuals had a higher risk of CVE compared with controls. These findings further highlight the importance of the early assessment of risk for CVD.
Tuberous Sclerosis Complex Patients’ Needs and Difficulties—Results of TAND Questionnaire Analysis in Polish Adult Population
Authors: Marcinkowska Anna , Jóźwiak Sergiusz , Tarasewicz Agnieszka , Dębska-Ślizień Alicja , Szurowska Edyta
Published: November 2022

Introduction: Tuberous Sclerosis Complex (TSC) is a rare genetic disease. Around 90% of individuals with TSC present some neuropsychiatric manifestations (TSC-associated neuropsychiatric disorders, TAND). To date, none of the studies have focused on the TAND profile of the adult population. Thus, the aim of the study was to describe their potential specific needs and difficulties, including differences in cohorts with or without epilepsy and/or intellectual disability. Method: The Polish version of the TAND Checklist was used for assessment of individuals with TSC. Participants had to meet the criteria for diagnosis of TSC. One hundred adult participants (forty-eight males/ fifty-two females; mean age 32.33 ± 11.29) were enrolled in the study. Epilepsy was present in 71% of patients; intellectual disability occurred in a total of 37%. Results: Only 11% of individuals received complete TAND features examination in the past. Moreover, 91.5 of the subjects had four and more TAND symptoms. Intellectually disabled patients and those with epilepsy had more neuropsychiatric problems than epilepsy-free subjects. Conclusions: Findings reveal that TANDs are common in adults with TSC and are underdiagnosed. Most individuals present several behavioural and cognitive problems. Among psychiatric disorders, the most common are ASD, depression, and anxiety disorder. TAND screening should be widely disseminated and applied in clinical practice for early identification, prevention, and rehabilitation of their difficulties. TAND is one of the most significant issues affecting the quality of life of TSC patients and their carers.
Role of Peripheral Inflammatory Markers in Patients with Acute Headache Attack to Differentiate between Migraine and Non-Migraine Headache
Authors: Lee Sang-Hwa , Kim Jong-Ho , Kwon Young-Suk , Sohn Jong-Hee
Published: November 2022

Although the potential relationship between headaches, particularly migraine, and peripheral inflammatory markers (PIMs) has been investigated, it is unclear whether PIMs are involved in the pathogenesis of migraine or can differentiate it from non-migraine headaches (nMHs). Using 10 years of data from the Smart Clinical Data Warehouse, patients who visited the neurology outpatient department (OPD) within 30 days after visiting the emergency room (ER) for headaches were divided into migraine and nMH groups, the PIMs were compared including the neutrophil-to-lymphocyte (NLR), monocyte-to-lymphocyte (MLR), platelet-to-lymphocyte (PLR) ratios, and neutrophil-to-monocyte ratio (NMR). Of the 32,761 patients who visited the ER for headaches, 4005 patients visited the neurology OPD within 30 days. There were significant increases in the NLR, MLR, and NMR, but a lower PLR in the migraine and nMH groups than the controls. The NMR was significantly higher in the migraine than the nMH group. A receiver operating characteristic curve analysis showed that the ability of the NLR and NMR to differentiate between migraine and nMHs was poor, whereas it was fair between the migraine groups and controls. The elevated PIMs, particularly the NLR and NMR, during headache attacks in migraineurs suggest that inflammation plays a role in migraine and PIMs may be useful for supporting a migraine diagnosis.
New Efficient Method for Hysteroscopic Isthmoplasty: Four Simple Steps Lead to a Significant Improvement in Bleeding Status
Authors: Huang Chien-Chu , Chiu Shao-Chih , Pan Chih-Ming , Huang Chun-Chung , Chang Cherry Yin-Yi , Chao Shih-Chi , Cho Der-Yang , Lin Wu-Chou
Published: November 2022

We demonstrate an effective reduction in postmenstrual spotting after our novel hysteroscopic isthmoplasty. This study included 66 patients with isthmocele-related postmenstrual spotting confirmed by sonography and diagnostic hysteroscopy between 2000 and 2017. Our new interventions included the following four steps: (1) make a resection gradient of the distal edge of the isthmocele from the ape of the isthmocele down to the cervical outer orifice; (2) resect the distal and proximal niches of the isthmocele; (3) electrocauterize the distal and proximal sides (not only the niche bottom) of the small cave on the scar side of the isthmocele; (4) manage the isthmocele until it is largely connected to the cavity. In our results, all patients underwent extensive hysteroscopic repair of newly hysteroscopic isthmoplasty without any intra- or postoperative complications. After final hysteroscopic repair modification, prolonged menstrual spotting was significantly decreased in 98.2% (53/54) of the patients, and the total number of bleeding days per menstrual cycle significantly decreased from a mean of 15.38 ± 3.3 days to 6.4 ± 1.9 days postoperatively (p < 0.001). Our four-step hysteroscopic technique successfully resolved prolonged menstrual spotting in over 90% of the patients, exceeding the resolution rates of 60–85% achieved with other hysteroscopic techniques used to treat symptomatic isthmocele. No patients experience recurrence after long-term follow up. Four simple steps led to a significant improvement in bleeding status.
Remdesivir for Patients Hospitalized with COVID-19 Severe Pneumonia: A National Cohort Study (Remdeco-19)
Authors: Zerbit Jeremie , Detroit Marion , Chevret Sylvie , Pene Frederic , Luyt Charles-Edouard , Ghosn Jade , Eyvrard Frederic , Martin-Blondel Guillaume , Sarton Benjamine , Clere-Jehl Raphael , Moine Pierre , Cransac Amelie , Andreu Pascal , Labruyère Marie , Albertini Laetitia , Huon Jean-François , Roge Pauline , Bernard Lise , Farines-Raffoul Magali , Villiet Maxime , Venet Arnaud , Dumont Louis Marie , Kaiser Jean-Daniel , Chapuis Claire , Goehringer François , Barbier François , Desjardins Stephane , Benzidi Younes , Abbas Nora , Guerin Corinne , Batista Rui , Llitjos Jean-François , Kroemer Marie
Published: November 2022

Background. Given the rapidly evolving pandemic of COVID-19 in 2020, authorities focused on the repurposing of available drugs to develop timely and cost-effective therapeutic strategies. Evidence suggested the potential utility of remdesivir in the framework of an early access program. REMDECO-19 is a multicenter national cohort study assessing the ability of remdesivir to improve the outcome of patients hospitalized with COVID-19. Methods. We conducted a retrospective real-life study that included all patients from the early access program of remdesivir in France. The primary endpoint was the clinical course evolution of critically ill and hospitalized COVID-19 patients treated with remdesivir. Secondary endpoints were the SOFA score evolution within 29 days following the admission and mortality at 29 and 90 days. Results. Eighty-five patients were enrolled in 22 sites from January to April 2020. The median WHO and SOFA scores were respectively reduced by two and six points between days 1 and 29. Improvement in the WHO-CPS and the SOFA score were observed in 83.5% and 79.3% of patients, respectively, from day 10. However, there was no effect of remdesivir on the 90-day survival based on the control cohort for hospitalized COVID-19 patients with invasive ventilation. Conclusions. SOFA score appeared to be an attractive approach to assess remdesivir efficacy and stratify its utilization or not in critically ill patients with COVID-19. This study brings a new clinical benchmark for therapeutic decision making and supports the use of remdesivir for some hospitalized COVID-19 patients.
Not to Rush—Laboratory Parameters and Procedural Complications in Patients Undergoing Left Atrial Appendage Closure
Authors: Zweiker David , Fiedler Lukas , Toth Gabor G. , Strouhal Andreas , Delle-Karth Georg , Stix Guenter , Gabriel Harald , Binder Ronald K. , Rammer Martin , Pfeffer Michael , Vock Paul , Lileg Brigitte , Steinwender Clemens , Sihorsch Kurt , Hintringer Florian , Mueller Silvana , Barbieri Fabian , Martinek Martin , Tkalec Wolfgang , Verheyen Nicolas , Ablasser Klemens , Zirlik Andreas , Scherr Daniel
Published: November 2022

Background: As a preventive procedure, minimizing periprocedural risk is crucially important during left atrial appendage closure (LAAC). Methods: We included consecutive patients receiving LAAC at nine centres and assessed the relationship between baseline characteristics and the acute procedural outcome. Major procedural complications were defined as all complications requiring immediate invasive intervention or causing irreversible damage. Logistic regression was performed and included age and left-ventricular function. Furthermore, the association between acute complications and long-term outcomes was evaluated. Results: A total of 405 consecutive patients with a median age of 75 years (37% female) were included. 47% had a history of stroke. Median CHA2DS2-VASc score was 4 (interquartile range, 3–5) and the median HAS-BLED score was 3 (2–4). Major procedural complications occurred in 7% of cases. Low haemoglobin (OR 0.8, 95% CI 0.65–0.99 per g/dL, p = 0.040) and end-stage kidney disease (OR 13.0, CI 2.5–68.5, p = 0.002) remained significant in multivariate analysis. Anaemia (haemoglobin < 12 and < 13 g/dL in female and male patients) increased the risk of complications 2.2-fold. Conclusions: The major complication rate was low in this high-risk patient population undergoing LAAC. End-stage kidney disease and low baseline haemoglobin were independently associated with a higher major complication rate.
The Enhanced Moisture Absorption and Tensile Strength of PVA/Uncaria gambir Extract by Boric Acid as a Highly Moisture-Resistant, Anti-UV, and Strong Film for Food Packaging Applications
Authors: Rahmadiawan Dieter , Abral Hairul , Railis Razan Muhammad , Iby Ilham Chayri , Mahardika Melbi , Handayani Dian , Natrana Khiky Dwi , Juliadmi Dian , Akbar Fazhar
Published: November 2022

There is an increasing demand for food packaging materials that are safe for the environment and human health. Pure polyvinyl alcohol (PVA) film is non-toxic and transparent but has poor UV-light shielding, thermal and moisture resistance, and antibacterial activity. Our previous work prepared and characterized a biofilm derived from PVA and edible Uncaria gambir extract (UG). The film has antibacterial properties and is anti-UV and flexible. However, UG is hydrophilic, making this film have low moisture absorption. To improve these properties, we trialed adding boric acid (BA) and UG into the PVA. This present study aims to characterize pure PVA film and blend films resulting from mixing PVA (10%), BA (0.5%), and UG (1%). It was found that the PVA/UG/BA film presented the best performance in terms of UV light absorption, tensile properties, thermal and moisture resistance, and antibacterial activity. This blend sample absorbs about 98% of the UV light at 400 nm wavelength without significantly sacrificing transparency. These findings indicate that UG and BA could be advantageous in the preparation of moisture and thermal-resistant UV shielding films with low toxicity and high antibacterial properties based on PVA. They were also found to be strong enough for food packaging applications.
Optimization and Prediction of Mechanical Characteristics on Vacuum Sintered Ti-6Al-4V-SiCp Composites Using Taguchi’s Design of Experiments, Response Surface Methodology and Random Forest Regression
Authors: Hegde Adithya Lokesh , Shetty Raviraj , Chiniwar Dundesh S , Naik Nithesh , Nayak Madhukara
Published: November 2022

Today, among emerging materials, metal matrix composites, due to their excellent properties, have an increasing demand in the field of aerospace and automotive industries. However, the difficulties associated with the processing of these composites have been a challenge to manufacturing industries due to inhomogeneous mixing of the matrix with the reinforcement, oxidation, and microstructural phase transformation during processing. Hence, in this paper, Ti-6Al-4V reinforced with SiCp has been processed through a specially developed compression molding, followed by vacuum sintering. The main objective of this paper was to determine the favorable vacuum sintering conditions for Ti-6Al-4V reinforced with 15 Wt. % SiCp composites under a different aging temperature (°C), aging time (h), heating rate (°C/min), and cooling rate (°C /min) to improve the process output parameters such as the hardness, surface roughness, and to reduce the porosity using Taguchi’s Design of Experiments. Finally, the response surface methodology and random forest regression have been used to predict the optimum process output parameters. From the extensive experimentation and understanding gained from Taguchi’s Design of Experiments, the response surface methodology and random tree regression approach can be successfully used to predict the hardness, porosity, and surface roughness during the processing of Ti-6Al-4V-SiCp composites.
Internal Cavitating Flow Distribution and Performance Comparison of a Disc Pump with Radial Straight Blade
Authors: Zhang Weibin , Li Hong , Shen Kun , Jiang Qifeng , Gérard Bois
Published: November 2022

Disc pumps with radial straight blade are unconventional designs that have been developed for hard-to-pump mixtures for many industrial applications. They are frequently used in chemical industries, sub-sea petroleum pumping systems with multi-phase media and so on. Due to the radial straight blade arrangement, they may suffer from cavitation onsets when being used under different pressure conditions. To study the influence of cavitation characteristic inside the present disc pump flow passages, experimental data analysis on a radial blade disc pump for a wide flow rate range (0 to 110 m3/h) is obtained. In addition, the Reynolds averaged (RANS) approach using the RNG k-ε turbulence model, is carried out under different working conditions with and without cavitation modelling. Two different pump meshing models are also implemented: the first one without hub and shroud side channels between the discs and the volute casing, the second one with both side channels corresponding to the complete real case geometry. The comparisons between experimental and numerical results reveal first that the complete geometry meshing must be used to recover the experimental performance curve especially at low flow rates whatever the rotational speed. Secondly, the cavitation effect is found to take place in precise locations (straight blade and connection column) inside the impeller at high rotational speed and high flow rates. Their effects correspond to the unexpected performance curve deterioration found for the highest rotational speed and high flow rates. Moreover, with the decrease of the inlet absolute pressure, the cavitation degree becomes serious, and the coupling phenomenon of straight blade cavitation and connection column cavitation is formed, which enlarges the scope and enhances the degree of cavitation. The present study proposes a data reduction procedure when open loop experimental testing procedure is used for specific disc pump design and the importance of the impeller-side channels interactions on head pump decrease at low flow coefficients.
Estimation of the Benthic Habitat Zonation by Photo-Quadrat Image Analysis along the Fringing Reef of Weno Island, Chuuk, Micronesia
Authors: Kim Taihun , Lee Dae-Won , Kim Han-Jun , Jung Yun-Hwan , Choi Young-Ung , Oh Jung-Hee , Kim Tae-Hoon , Kang Do-Hyung , Park Heung-Sik
Published: November 2022

Benthic habitat zonation is described from in situ observations and seabed photographs taken from the coastal area of Weno Island, Chuuk, Micronesia. Habitat groups, types, and boundaries are defined by visible substratum characteristics (i.e., in situ and by digital imaging of photo-quadrats along transect lines), and by cluster and ordination analyses using relative coverage percentage of identified classification categories. The statistical similarity between habitat groups is determined by Analysis of similarity (ANOSIM). Benthic habitat groups with significant influence on the determination of habitat type are isolated by the similarity percentage (SIMPER) test. In addition to the standard practices of using transect lines and collecting data in accordance with the already well-implemented and thoroughly-tested benthic habitat classification scheme for tropical reef monitoring, we applied simple statistics that enable comparative data interpretation. Our simple, repeatable methods provide a framework for benthic habitat-related monitoring research that allows the comparison of results across regions.