bluuebunny/crossref_metadata_2025 huggingface dataset:
The Renoprotective Mechanisms of Sodium-Glucose Cotransporter-2 Inhibitors (SGLT2i)—A Narrative Review
Authors: Iordan Liana , Gaita Laura , Timar Romulus , Avram Vlad , Sturza Adrian , Timar Bogdan
Published: June 2024

Chronic kidney disease (CKD) is a noncommunicable condition that has become a major healthcare burden across the globe, often underdiagnosed and associated with low awareness. The main cause that leads to the development of renal impairment is diabetes mellitus and, in contrast to other chronic complications such as retinopathy or neuropathy, it has been suggested that intensive glycemic control is not sufficient in preventing the development of diabetic kidney disease. Nevertheless, a novel class of antidiabetic agents, the sodium-glucose cotransporter-2 inhibitors (SGLT2i), have shown multiple renoprotective properties that range from metabolic and hemodynamic to direct renal effects, with a major impact on reducing the risk of occurrence and progression of CKD. Thus, this review aims to summarize current knowledge regarding the renoprotective mechanisms of SGLT2i and to offer a new perspective on this innovative class of antihyperglycemic drugs with proven pleiotropic beneficial effects that, after decades of no significant progress in the prevention and in delaying the decline of renal function, start a new era in the management of patients with CKD.
Comparative Study of Condensed and Hydrolysable Tannins during the Early Stages of Zebrafish Development
Authors: La Pietra Alessandra , Imperatore Roberta , Coccia Elena , Mobilio Teresa , Ferrandino Ida , Paolucci Marina
Published: June 2024

In this study, we present data on the effects of condensed tannins (CTs) and hydrolysable tannins (HTs), polyphenols extracted from plants, at different concentrations on zebrafish development to identify the range of concentrations with toxic effects. Zebrafish embryos were exposed to CTs and HTs at two different concentration ranges (5.0–20.0 μgL−1 and 5.0–20.0 mgL−1) for 72 h. The toxicity parameters were observed up to 72 h of treatment. The uptake of CTs and HTs by the zebrafish larvae was assessed via HPLC analysis. A qRT-PCR analysis was performed to evaluate the expressions of genes cd63, zhe1, and klf4, involved in the hatching process of zebrafish. CTs and HTs at 5.0, 10.0, and 20.0 μgL−1 were not toxic. On the contrary, at 5.0, 10.0, and 20.0 mgL−1, HTs induced a delay in hatching starting from 48 h of treatment, while CTs showed a delay in hatching mainly at 48 h. The analysis of gene expression showed a downregulation in the group exposed to HTs, confirming the hatching data. We believe that this study is important for defining the optimal doses of CTs and HTs to be employed in different application fields such as the chemical industry, the animal feed industry, and medical science.
Anti-Influenza A Potential of Tagetes erecta Linn. Extract Based on Bioinformatics Analysis and In Vitro Assays
Authors: Kim Minjee , Nowakowska Aleksandra , Kim Jaebum , Kim Young Bong
Published: June 2024

Tagetes erecta Linn. (TE) is traditionally used to treat cardiovascular, renal, and gastrointestinal diseases. In this study, we investigated the active compounds and targets of TE extract that may exert antiviral effects against influenza A. Active compounds and targets of TE extract were identified using the Traditional Chinese Medicine Systems Pharmacology database (TCSMP). The influenza A-related gene set was screened using GeneCards and the Kyoto Encyclopedia of Genes and Genomes (KEGG). A protein–protein interaction (PPI) network was built to establish the hub targets. Pathway and target studies were conducted using Gene Expression Omnibus (GEO). The interactions between active compounds and potential targets were assessed by molecular docking. An in vitro study was performed using antiviral and plaque reduction assays. From the compound and target search, we identified 6 active compounds and 95 potential targets. We retrieved 887 influenza-associated target genes and determined 14 intersecting core targets between TE and influenza. After constructing a compound–target network, we discovered lutein and beta-carotene to be the key compounds. Next, PPI network analysis identified the top three hub genes associated with influenza (IL-6, HIF1A, and IL-1β). Similarly, GEO analysis revealed IL-6, TGFB1, and CXCL8 to be the top three target genes. In our docking study, we identified that lutein and IL-6 had the strongest bindings. Our in vitro experimental results revealed that the TE extract exhibited therapeutic rather than prophylactic effects on influenza disease. We identified lutein as a main active compound in TE extract, and IL-6 as an important target associated with influenza, by using data mining and bioinformatics. Our in vitro findings indicated that TE extract exerted protective properties against the influenza A virus. We speculated that lutein, as a key active component in TE extract, is largely responsible for its antiviral effects. Therefore, we suggest TE extract as an alternative in the treatment of influenza.
New Potential Pharmacological Options for Endometriosis Associated Pain
Authors: García-Izquierdo Laura , Marín-Sánchez Pilar , García-Peñarrubia Pilar , Martínez-Esparza María
Published: June 2024

Endometriosis is a chronic inflammatory disorder characterized by the abnormal growth of endometrial-like tissue outside the uterine cavity, affecting 10–15% of women of reproductive age. Pain is the most common symptom. Treatment options include surgery, which has limited effectiveness and high recurrence rates, and pharmacotherapy. Hormonal therapies, commonly used for symptom management, can have side effects and contraceptive outcomes, contributing to the infertility associated with endometriosis, with pain and lesions often reappearing after treatment cessation. Among its etiological factors, immunological and inflammatory dysregulation plays a significant role, representing an interesting target for developing new therapeutic strategies. This review critically analyzes recent studies to provide an updated synthesis of ongoing research into potential new pharmacotherapies focusing on lesion progression, pain relief, and improving quality of life. Immunotherapy, natural anti-inflammatory and antioxidant compounds and drug repurposing show promise in addressing the limitations of current treatments by targeting immunological factors, potentially offering non-invasive solutions for managing pain and infertility in endometriosis. Promising results have been obtained from in vitro and animal model studies, but clinical trials are still limited. More effort is needed to translate these findings into clinical practice to effectively reduce disease progression, alleviate pain symptoms and preserve the reproductive capacity, improving patients’ overall wellbeing.
The Impact of Neoadjuvant Chemotherapy on Ovarian Cancer Tumor Microenvironment: A Systematic Review of the Literature
Authors: Spagnol Giulia , Ghisoni Eleonora , Morotti Matteo , De Tommasi Orazio , Marchetti Matteo , Bigardi Sofia , Tuninetti Valentina , Tasca Giulia , Noventa Marco , Saccardi Carlo , Tozzi Roberto , Dangaj Laniti Denarda
Published: June 2024

Immunotherapy, particularly the use of immune checkpoint inhibitors (ICIs), has shown limited efficacy in treating ovarian cancer (OC), possibly due to diverse T cell infiltration patterns in the tumor microenvironment. This review explores how neoadjuvant chemotherapy (NACT) impacts the immune landscape of OC, focusing on tumor-infiltrating lymphocytes (TILs), PD-1/PD-L1 expression, and their clinical implications. A comprehensive literature search across four databases yielded nine relevant studies. These studies evaluated stromal (sTILs) and intra-epithelial (ieTILs) TILs before and after NACT. sTIL responses varied, impacting prognostic outcomes, and ieTILs increased in some patients without clear survival associations. PD-L1 expression after NACT correlated with improved overall survival (OS), and increases in granzyme B+ and PD-1 correlated with longer progression-free survival (PFS). Remarkably, reduced FoxP3+ TILs post-NACT correlated with better prognosis. NACT often increases sTIL/ieTIL and CD8+ subpopulations, but their correlation with improved PFS and OS varies. Upregulation of co-inhibitory molecules, notably PD-L1, suggests an immunosuppressive response to chemotherapy. Ongoing trials exploring neoadjuvant ICIs and chemotherapy offer promise for advancing OC treatment. Standardized measurements assessing TIL density, location, and heterogeneity are crucial for addressing genetic complexity and immunological heterogeneity in OC.
Mapping Microplastics in Humans: Analysis of Polymer Types, and Shapes in Food and Drinking Water—A Systematic Review
Authors: Vdovchenko Alena , Resmini Marina
Published: June 2024

Microplastics (MPs) pervade the environment, infiltrating food sources and human bodies, raising concerns about their impact on human health. This review is focused on three key questions: (i) What type of polymers are humans most exposed to? (ii) What are the prevalent shapes of MPs found in food and human samples? (iii) Are the data influenced by the detection limit on the size of particles? Through a systematic literature analysis, we have explored data on polymer types and shapes found in food and human samples. The data provide evidence that polyester is the most commonly detected polymer in humans, followed by polyamide, polyurethane, polypropylene, and polyacrylate. Fibres emerge as the predominant shape across all categories, suggesting potential environmental contamination from the textile industry. Studies in humans and drinking water reported data on small particles, in contrast to larger size MPs detected in environmental research, in particular seafood. Discrepancies in size detection methodologies across different reports were identified, which could impact some of the discussed trends. This study highlights the need for more comprehensive research on the interactions between MPs and biological systems and the effects of MPs on toxicity, together with standardised analytical methodologies to accurately assess contamination levels and human exposure. Understanding these dynamics is essential for formulating effective strategies to mitigate the environmental and health implications of MP pollution.
Exosome Therapy: A Novel Approach for Enhancing Estrogen Levels in Perimenopause
Authors: Alkhrait Samar , Omran Mervat M. , Ghasroldasht Mohammad Mousaei , Park Hang-Soo , Katkhuda Riham , Al-Hendy Ayman
Published: June 2024

Perimenopause significantly impacts women’s health globally, often managed with hormone replacement therapy (HRT) despite the associated risks. This study explores a novel alternative exosome therapy, aimed at stimulating estrogen production in ovarian tissues, thus offering a potential non-hormonal treatment for perimenopausal symptoms. Employing ex vivo methodologies, ovarian cortex specimens from perimenopausal women were treated with exosomes derived from human umbilical cord mesenchymal stem cells and cultured under specific conditions (patent number: PCT/US2022/073467). The exosomes were produced under cyclic guanosine monophosphate (cGMP) conditions, ensuring high safety standards. Estrogen levels were quantified using enzyme-linked immunosorbent assay (ELISA), and gene expression changes in estrogen and follicle-stimulating hormone (FSH) receptors were assessed via quantitative polymerase chain reaction (PCR). Immunohistochemistry (IHC) was utilized to evaluate cellular proliferation and apoptotic markers. The results indicated a significant increase in estrogen levels and estrogen receptor-alpha (Erα) expression in treated tissues compared to controls. Additionally, a decrease in apoptotic markers and an increase in cellular proliferation markers were observed. These findings suggest that exosome therapy can effectively enhance estrogen production and modulate receptor sensitivity in perimenopausal ovarian tissues. This approach could serve as a safer alternative to HRT, aligning with the body’s natural regulatory mechanisms and potentially offering a more effective treatment option for managing perimenopausal symptoms.
Cordycepin Enhances the Therapeutic Efficacy of Doxorubicin in Treating Triple-Negative Breast Cancer
Authors: Huang Haichen , Li Xiaomin , Wu Wenya , Liu Chengyi , Shao Yunhe , Wu Xiaoping , Fu Junsheng
Published: June 2024

Triple-negative breast cancer (TNBC) is a subtype of breast cancer with high mortality and poor prognosis. Meanwhile, doxorubicin, a chemotherapeutic agent for triple-negative breast cancer, has poor sensitivity. The objective of this study was to examine the effect of cordycepin on doxorubicin sensitivity and efficacy in the TNBC xenograft model and explore the relevant molecular pathways. The combination of the drugs in nude mice carrying MDA-MB-231 xenografts significantly reduced the volume, size, and weight of xenografts and improved the tumor inhibition rate. The drug combination was significantly more effective than cordycepin or doxorubicin alone, reflecting the fact that cordycepin enhanced the anti-tumor effects of doxorubicin in MDA-MB-231 xenografts. At the same time, the monitoring of several biological parameters failed to detect any obvious side effects associated with this treatment. After predicting the importance of the TNF pathway in inhibiting tumor growth using network pharmacology methods, we verified the expression of TNF pathway targets via immunohistochemistry and quantitative PCR. Furthermore, a TNF-α inhibitor was able to abrogate the beneficial effects of cordycepin and doxorubicin treatment in MDA-MB-231 cells. This clearly indicates the role of TNF-α, or related molecules, in mediating the therapeutic benefits of the combined treatment in animals carrying TNBC xenografts. The observations reported here may present a new direction for the clinical treatment of TNBC.
Unraveling the Crystal Structure of Sodium Tetrabenzylborate: Synthesis through the Sodium Borohydride Reduction of Benzaldehyde in the Solid State
Authors: Castilla-Martinez Carlos A. , Granier Dominique , Yot Pascal G. , Demirci Umit B.
Published: June 2024

We present the synthesis, characterization, and crystal structure of sodium tetrabenzylborate, a novel tetraalkoxyborate obtained via a direct mechanochemical reaction between benzaldehyde and sodium borohydride at room temperature. The molecular and crystal structures of this borate were investigated using 11B MAS NMR, IR spectroscopy, differential scanning calorimetry (DSC), and X-ray diffraction (XRD) analyses. Crystalline sodium tetrabenzylborate exists in two different crystal structures, which were elucidated using powder- and single-crystal-XRD analyses. At a low temperature (e.g., −100 °C), sodium tetrabenzylborate crystallizes in the monoclinic system with the space group P21 (No. 4), but at room temperature, it displays a crystallization in the tetragonal system with the space group I4¯ (No. 82). According to the DSC analysis, the phase transition occurs at −45 °C. Upon hydrolysis, sodium tetrabenzylborate undergoes direct transformation into benzyl alcohol, thereby confirming the ability of sodium borohydride to convert an aldehyde into its primary alcohol analog. The key findings from our analyses are presented herein.
Hypoxia-Induced Pulmonary Injury—Adrenergic Blockade Attenuates Nitrosative Stress, and Proinflammatory Cytokines but Not Pulmonary Edema
Authors: Riha Isabel , Salameh Aida , Hoschke Annekathrin , Raffort Coralie , Koedel Julia , Rassler Beate
Published: June 2024

Hypoxia can induce pulmonary edema (PE) and inflammation. Furthermore, hypoxia depresses left ventricular (LV) inotropy despite sympathetic activation. To study the role of hypoxic sympathetic activation, we investigated the effects of hypoxia with and without adrenergic blockade (AB) on cardiovascular dysfunction and lung injury, i.e., pulmonary edema, congestion, inflammation, and nitrosative stress. Eighty-six female rats were exposed for 72 h to normoxia or normobaric hypoxia and received infusions with NaCl, prazosin, propranolol, or prazosin–propranolol combination. We evaluated hemodynamic function and performed histological and immunohistochemical analyses of the lung. Hypoxia significantly depressed LV but not right ventricular (RV) inotropic and lusitropic functions. AB significantly decreased LV function in both normoxia and hypoxia. AB effects on RV were weaker. Hypoxic rats showed signs of moderate PE and inflammation. This was accompanied by elevated levels of tumor necrosis factor α (TNFα) and nitrotyrosine, a marker of nitrosative stress in the lungs. In hypoxia, all types of AB markedly reduced both TNFα and nitrotyrosine. However, AB did not attenuate PE. The results suggest that hypoxia-induced sympathetic activation contributes to inflammation and nitrosative stress in the lungs but not to PE. We suggest that AB in hypoxia aggravates hypoxia-induced inotropic LV dysfunction and backlog into the pulmonary circulation, thus promoting PE.
Robotic Mitral Valve Repair: Impact of Experience on Results and Complex Mitral Disease Treatment
Authors: Lio Antonio , Russo Marco , Sangiorgi Beatrice , Nicolò Francesca , Chirichilli Ilaria , Irace Francesco , Ranocchi Federico , Musumeci Francesco
Published: June 2024

Background/Objectives: Robotically assisted mitral valve (MV) surgery is the least invasive surgical approach to the MV. The aim of the present study is to report our experience with robotically assisted MV repair, trying to define how experience could impact on postoperative results. Methods: This is a retrospective study on 144 patients who underwent robotic MV repair from November 2011 to March 2023. Patients were divided in two groups: Group 1, including 39 patients (November 2011–January 2013) operated using the Da Vinci Si system, and Group 2, including 105 patients operated (February 2020–March 2023) using the new Da Vinci Xi system. Results: Mean age was 58 ± 10 years. Increased use of external aortic clamp was observed in Group 2. A significant reduction of surgical times was observed: cardiopulmonary bypass time was 155 ± 44 min in Group 1 and 121 ± 36 min in Group 2 (p = 0.002), whereas cross-clamp time was 112 ± 25 min in Group 1 and 68 ± 39 min in Group 2 (p < 0.001). In-hospital mortality was 0.7%, and 10-year survival was 96 ± 2%. Freedom from reoperation was 100%. A higher percentage of complex and most complex MV repairs were performed in Group 2 (36% in Group 1 vs. 52% in Group 2, p = 0.001). Conclusions: Robotic-assisted MV repair is associated with excellent results. Experience is a key element to overcome the limitations of this technology. Finally, the robotic platform could improve results in difficult MV repair.
Comparison of TEVA vs. PRAAT in the Acoustic Characterization of the Tracheoesophageal Voice in Laryngectomized Patients
Authors: Klein-Rodríguez Alejandro , Cabo-Varela Irma , Vázquez-de la Iglesia Francisco , Chiesa-Estomba Carlos M. , Mayo-Yáñez Miguel
Published: June 2024

Background: Previous studies have assessed the capability of PRAAT for acoustic voice analysis in total laryngectomized (TL) patients, although this software was designed for acoustic analysis of laryngeal voice. Recently, we have witnessed the development of specialized acoustic analysis software, Tracheoesophageal Voice Analysis (TEVA). This study aims to compare the analysis with both programs in TL patients. Methods: Observational analytical study of 34 TL patients where a quantitative acoustic analysis was performed for stable phonation with vowels [a] and [i] as well as spectrographic characterization using the TEVA and PRAAT software. Results: The Voice Handicap Index (VHI-10) showed a mean score of 11.29 ± 11.16 points, categorized as a moderate handicap. TEVA analysis found lower values in the fundamental frequency vs. PRAAT (p < 0.05). A significant increase in shimmer values was observed with TEVA (>20%). No significant differences were found between spectrographic analysis with TEVA and PRAAT. Conclusions: Tracheoesophageal speech is an alaryngeal voice, characterized by a higher degree of irregularity and noise compared to laryngeal speech. Consequently, it necessitates a more tailored approach using objective assessment tools adapted to these distinct features, like TEVA, that are designed specifically for TL patients. This study provides statistical evidence supporting its reliability and suitability for the evaluation and tracking of tracheoesophageal speakers.
Pericardial and Pleural Effusions in Non-ICU Hospitalized Patients with COVID-19—A Retrospective Single-Center Study
Authors: Mangaloiu David V. , Tilișcan Cătălin , Răriș Alexandra D. , Negru Anca R. , Molagic Violeta , Vișan Constanta A. , Stratan Laurențiu M. , Mihai Nicoleta , Aramă Ștefan S. , Aramă Victoria
Published: June 2024

Background: Pericardial and pleural effusions are two complications recently described in patients hospitalized with COVID-19 infections. There are several mechanisms that have been proposed and refer to SARS-CoV-2’s capacity to bind to cell surfaces via various receptors and its broad tissue tropism that might cause significant complications. The aim of the present study is to evaluate the incidence of pericardial and pleural effusions during COVID-19 infection as well as to determine the risk factors associated with these complications. Methods: We conducted a retrospective single-center study that included 346 patients admitted to the National Institute of Infectious Disease “Prof. Dr. Matei Bals” (Bucharest, Romania), from 1 January to 25 May 2021, during the third wave of the pandemic. Socio-demographic and anthropometric data were collected for each patient. The patients were evaluated clinically, biologically, and radiologically within 48 h of admission. Patients were divided into 3 groups: (1) patients with pericardial effusions—18; (2) patients with pleural effusions—28; (3) patients without pericardial/pleural effusions—294. Results: After exclusion criteria were applied, 337 patients were analyzed. The median age of the participants was 58.26 ± 14.58 years. More than half of the hospitalized patients had associated respiratory failure (61.5%), of which 2.7% had a critical form of the disease and 58.8% had a severe form. The cumulative percentage for pericardial and pleural effusions for the study group was 12.8% (43 patients out of 337). The prevalence of pericardial effusion was 5.3%, twice more frequent among male respondents. Pleural effusion was identified in 8.3% patients. Most patients had unilateral effusion (17), compared to 11 patients who had bilateral involvement. Based on laboratory results, patients with pericardial and pleural effusions exhibited increased levels of C reactive protein, erythrocyte sedimentation rate, NT proBNP, and a higher value of neutrophil/lymphocyte count ratio. In contrast to patients without pleural and pericardial effusions, those with these symptoms experienced a higher frequency of severe or critical illness and longer hospital stays. Conclusions: Pericardial and pleural effusions can complicate COVID-19 infections. In our study, the prevalence of pericardial and pleural effusions in hospitalized patients was low, being associated with the same comorbidities and a number of clinical and biological parameters.
Influence of Schneiderian Membrane Perforation on Implant Survival Rate: Systematic Review and Meta-Analysis
Authors: Schiavo-Di Flaviano Verónica , Egido-Moreno Sonia , González-Navarro Beatriz , Velasco-Ortega Eugenio , López-López José , Monsalve-Guil Loreto
Published: June 2024

Background: Maxillary sinus lift is a well-documented and accepted technique in the rehabilitation of the posterior maxilla. Schneiderian membrane perforation is the most common complication and may occur in between 7% and 56% of cases. Different materials and techniques have been described to achieve reparation of the perforation. The aim of this study was to establish whether the perforation of the Schneiderian membrane and its repair during maxillary sinus lift surgery results in a lower implant survival rate compared to those cases where the membrane has not been perforated. Materials and methods: A systematic review and meta-analysis of studies assessing the survival rate of implants placed in regenerated sinus using the lateral window approach, where the perforation of the Schneiderian membrane occur, was carried out. Statistical analysis was performed with Open Meta-Analyst, calculating the odds ratio of implants placed in perforated sinuses and non-perforated sinuses. Results: Ten articles were included in the qualitative analysis and seven articles in the quantitative analysis or meta-analysis. A total of 1224 maxillary sinus augmentation surgeries were performed without perforation of the Schneiderian membrane and 2725 implants were placed; 62 implants failed during the follow-up period with an overall survival rate of 97.7%. In 480 perforated sinuses, 1044 implants were placed, of which 30 failed; the overall survival rate was 97.1%. There were no significant differences between the implant survival rate of the implants in the two groups (OR = 0.78; CI = 0.49–2.23; p = 0.28 and I2 heterogeneity: 0%, p = 0.44). Conclusions: Schneiderian membrane perforation, as long as it is repaired, does not appear to negatively influence implant survival rate. Membrane perforation should not be considered a reason to abort the procedure or an absolute contraindication to implant placement.
Increased Risk for Infections and Allergic Disease in Hereditary Hemorrhagic Telangiectasia
Authors: Droege Freya , König Jochem , Lang Karl S. , Jablonska Jadwiga , Pylaeva Ekaterina , Huckenbeck Carolin , Wrobeln Anna , Duerig Inga , Thangavelu Kruthika , Lang Stephan , Geisthoff Urban
Published: June 2024

Background/Objectives: Hereditary hemorrhagic telangiectasia (HHT) is a rare disorder characterized by dilated blood vessels. Different immunological changes have been described in these patients. In this study, the predisposition of patients with HHT to infections and allergic diseases was assessed. Methods: Patients with HHT completed an online survey in English or German. Their data were compared to non-affected partners or friends. Results: A total of 430 out of 588 respondents with HHT answered our questions about infections and allergies. Patients with HHT suffered significantly more often from various types of allergies than their partners, especially type I allergies (n = 226/276, 82%), and had a higher risk for sinusitis, urinary tract infections, pulmonary infections, and abscesses. A total of 38% of the patients took antibiotics prior to dental or surgical procedures (n = 57/152), and, in 10% of these patients, pulmonary arteriovenous malformations (PAVMs) were not detected. On the other hand, 51% of patients with PAVM did not report a prophylactic antibiotic intake (n = 40/79). The patients who needed iron supplementations suffered more often from sepsis (OR: 9.00, 95%CI: 0.92–88.16). Conclusions: Compared to their non-affected controls, patients with HHT showed an increased risk for infections in different organs and allergic diseases. There is a need for campaigns raising greater awareness recommending prophylactic antibiotic intake in patients with PAVM.
Advancements in Predictive Tools for Primary Graft Dysfunction in Liver Transplantation: A Comprehensive Review
Authors: Gierej Piotr , Radziszewski Marcin , Figiel Wojciech , Grąt Michał
Published: June 2024

Orthotopic liver transplantation stands as the sole curative solution for end-stage liver disease. Nevertheless, the discrepancy between the demand and supply of grafts in transplant medicine greatly limits the success of this treatment. The increasing global shortage of organs necessitates the utilization of extended criteria donors (ECD) for liver transplantation, thereby increasing the risk of primary graft dysfunction (PGD). Primary graft dysfunction (PGD) encompasses early allograft dysfunction (EAD) and the more severe primary nonfunction (PNF), both of which stem from ischemia–reperfusion injury (IRI) and mitochondrial damage. Currently, the only effective treatment for PNF is secondary transplantation within the initial post-transplant week, and the occurrence of EAD suggests an elevated, albeit still uncertain, likelihood of retransplantation urgency. Nonetheless, the ongoing exploration of novel IRI mitigation strategies offers hope for future improvements in PGD outcomes. Establishing an intuitive and reliable tool to predict upcoming graft dysfunction is vital for early identification of high-risk patients and for making informed retransplantation decisions. Accurate diagnostics for PNF and EAD constitute essential initial steps in implementing future mitigation strategies. Recently, novel methods for PNF prediction have been developed, and several models for EAD assessments have been introduced. Here, we provide an overview of the currently scrutinized predictive tools for PNF and EAD evaluation strategies, accompanied by recommendations for future studies.
Headache and Associated Psychological Variables in Intensive Care Unit Nurses during the COVID-19 Pandemic: A Prospective Study
Authors: Gil-Almagro Fernanda , Carmona-Monge Francisco Javier , García-Hedrera Fernando José , Peñacoba-Puente Cecilia
Published: June 2024

(1) Background: Headaches in health professionals have been studied over the years. This has become even more relevant during the COVID-19 pandemic, due to their link with the use of masks, being female or working in highly complex units. However, their association with different personality traits has not been studied in healthcare workers (HCWs). The aim of this study was to assess the prevalence and evolution of headaches throughout the pandemic in Intensive Care Unit (ICU) nurses and to analyze their association with sociodemographic, occupational and personality variables as well as with other symptoms. (2) Methods: This was an observational, descriptive, longitudinal, prospective study with two periods of data collection (at the end of the containment phase and six months thereafter). A non-probabilistic convenience sampling was performed (n = 131). (3) Results: A high percentage of ICU nurses reported headaches during the first (71%) or second (79.4%) time point; more than half of the sample (58.8%) reported headaches over time (chronic headache). Although a higher prevalence of headaches was observed in women at both assessment points, no significant gender-related relationships were observed for headaches maintained across the two time points. Regarding the symptoms and personality variables, positive relationships were found between headaches and anxiety (p = 0.005), insomnia (p = 0.030) and emotional exhaustion (p = 0.022), and a negative relationship was found between headaches and hardiness (p = 0.031). (4) Conclusions: Our study highlights the importance of assessing occupational, psychological and emotional aspects in order to decrease the prevalence of headaches and thus improve the quality of the work life of nurses in such demanding environments as the ICU.
Single-Arm, Prospective, Interventional Study of Helicobacter pylori Eradication Rescue Therapy with Rifabutin, Metronidazole, and Vonoprazan
Authors: Sue Soichiro , Ikeda Ryosuke , Ikeda Aya , Sato Hiroki , Kaneko Hiroaki , Irie Kuniyasu , Maeda Shin
Published: June 2024

Background and Objective: Rescue Helicobacter pylori eradication can be challenging. Rifabutin (RBT) demonstrates high activity against Helicobacter pylori and is incorporated into various rescue eradication regimens. This exploratory study was performed to evaluate the efficacy and safety of a rescue regimen comprising RBT, metronidazole (MNZ), and vonoprazan (VPZ). Methods: This prospective, single-center, single-arm, interventional study was performed in Japan. Eligible patients were those who underwent failed primary eradication treatment (7-day treatment with three drugs: VPZ or a proton pump inhibitor [PPI], amoxicillin [AMPC], and clarithromycin) and secondary eradication treatment (7-day treatment with three drugs: VPZ or a PPI, AMPC, and MNZ) and those who were unable to receive first- and second-line therapy because of penicillin allergy. Twenty Helicobacter pylori-positive patients were treated with RBT (150 mg twice daily), MNZ (250 mg twice daily), and VPZ (20 mg twice daily) for 10 days (RBT-MNZ-VPZ therapy). Eradication success was evaluated using the urea breath test. Drug susceptibility test results were available in 16 patients. This study is registered in the Japan Registry of Clinical Trials (jRCT031220504). Results: The intention-to-treat (ITT) and per-protocol (PP) eradication rates of RBT-MNZ-VPZ therapy were 70% (90% confidence interval [CI]: 49.2%–86.0%) and 72.2% (95% CI: 50.2%–88.4%), respectively. In the MNZ-susceptible subgroup, the ITT (n = 8) and PP (n = 7) eradication rates were 100% (90% CI: 68.8%–100%) and 100% (90% CI: 65.2%–100%). In the MNZ-resistant subgroup, the ITT (n = 8) and PP (n = 7) eradication rates were both 62.5% (90% CI: 28.9%–88.9%). All infections were RBT-susceptible. Conclusions: These findings suggest that RBT-MNZ-VPZ therapy may be a promising rescue regimen, especially in MNZ- and RBT-susceptible infections or patients with penicillin allergy.
Recent Insights in Noninvasive Diagnostic for the Assessment of Kidney and Cardiovascular Outcome in Kidney Transplant Recipients
Authors: Falahat Peyman , Scheidt Uta , Pörner Daniel , Schwab Sebastian
Published: June 2024

Kidney transplantation improves quality of life and prolongs survival of patients with end-stage kidney disease. However, kidney transplant recipients present a higher risk for cardiovascular events compared to the general population. Risk assessment for graft failure as well as cardiovascular events is still based on invasive procedures. Biomarkers in blood and urine, but also new diagnostic approaches like genetic or molecular testing, can be useful tools to monitor graft function and to identify patients of high cardiovascular risk. Many biomarkers have been introduced, whereas most of these biomarkers have not been implemented in clinical routine. Here, we discuss recent developments in biomarkers and diagnostic models in kidney transplant recipients. Because many factors impact graft function and cardiovascular risk, it is most likely that no biomarker will meet the highest demands and standards. We advocate to shift focus to the identification of patients benefitting from molecular and genetic testing as well as from analysis of more specific biomarkers instead of finding one biomarker fitting to all patients.
Impact of Guideline-Directed Drug Therapy after ST-Elevation Myocardial Infarction on Outcome in Young Patients—Age and Sex-Specific Factors
Authors: Fischer Alicia Jeanette , Feld Jannik , Lange Stefan A. , Günster Christian , Dröge Patrik , Engelbertz Christiane , Ruhnke Thomas , Gerß Joachim , Reinecke Holger , Köppe Jeanette
Published: June 2024

Background: Specifically young women are at risk for a poor outcome after ST-elevation myocardial infarction (STEMI). We aimed to investigate sex- and age-specific differences in outcome and associate these results with adherence to a guideline-directed optimal medical therapy (OMT). Methods: Administrative insurance data (≈26 million insured) were screened for patients aged 18–60 years with STEMI. Patient demographics, details on in-hospital treatment, adherence to OMT and its effect on mortality were assessed. Adherence to OMT was analyzed using multistate models and an association of those with death was fitted using multivariable Cox regression models with time-dependent co-variables. Results: Overall, 59,401 patients (19.3% women), median age 52 (interquartile range 48, 56) presented with STEMI. Female sex was associated with a poor outcome early after STEMI (90-day mortality: odds ratio 1.22, 95% confidence interval (CI) 1.12–1.32, p < 0.001). Overall survival was reduced in women compared to same-aged men. The ten-year survival rate was 19.7% (18.1–21.2%) versus 19.6% (18.9–20.4%) in men (p < 0.001). Although long-term drug adherence was low, its intake was associated with a better outcome. Specifically younger women showed a markedly lower mortality when on OMT (hazard ratio (HR) 0.22 (95% CI 0.19–0.26) versus HR 0.31 (95% CI 0.28–0.33) in men, pint < 0.001). Conclusions: Specifically young women were at risk for a poor outcome in the early phase after STEMI. Although long-term adherence to OMT was low, it was generally associated with a lower mortality, specifically in women. Our findings emphasize on early and long-term preventive measures in all patients after STEMI.
Dissimilar Welding of Thick Ferritic/Austenitic Steels Plates Using Two Simultaneous Laser Beams in a Single Pass
Authors: Giudice Fabio , Missori Severino , Sili Andrea
Published: June 2024

Dissimilar welds between ferritic and austenitic stainless steels are widely used in industrial applications. Taking into account the issues inherent to arc welding, such as the high heat input and the need to carry out multiple passes in the case of thick plates, a procedure with two simultaneous laser beams (working in a single pass) and consumable inserts as filler metal has been considered. Particular attention was paid to the choice of the filler metal (composition and amount), as well as welding parameters, which are crucial to obtain the right dilution necessary for a correct chemical composition in the weld zone. The first experimental investigations confirmed the achievement of a good weldability of the dissimilar pair ASTM A387 ferritic/AISI 304L austenitic steel, having ascertained that the microstructure of the weld zone is austenitic with a little amount of residual primary ferrite, which is the best condition to minimize the risk of hot cracking.
Task-Driven Learning Downsampling Network Based Phase-Resolved Wave Fields Reconstruction with Remote Optical Observations
Authors: Mou Tianyu , Shen Zhipeng , Xue Guangshi
Published: June 2024

We develop a phase-resolved wave field reconstruction method by the learning-based downsampling network for processing large amounts of inhomogeneous data from non-contact wave optical observations. The Waves Acquisition Stereo System (WASS) extracts dense point clouds from ocean wave snapshots. We couple learning-based downsampling networks with the phase-resolved wave reconstruction algorithm, and the training task is to improve the wave reconstruction completeness ratio CR. The algorithm first achieves initial convergence and task-optimized performance on numerical ocean waves built by the linear wave theory model. Results show that the trained sampling network can lead to a more uniform spatial distribution of sampling points and improve CR at the observed edge regions far from the optical camera. Finally, we apply our algorithm to a natural ocean wave dataset. The average completeness ratio is improved over 30% at low sampling ratios (SR∈[2−9,2−7]) compared to the traditional FPS method and Random sampling method. Moreover, the relative residual between the final reconstructed wave and the natural wave is less than 15%, which provides an efficient tool for wave reconstruction in ocean engineering.
Medium-Term Effects of Dune Erosion and Longshore Sediment Transport on Beach–Dune Systems Evolution
Authors: Ferreira Ana Margarida , Coelho Carlos , Silva Paulo A.
Published: June 2024

Beach–dune systems are highly dynamic features of the coastal system, the evolution of which is influenced by several processes that occur at very different spatial and temporal scales. To mitigate shoreline retreat that threatens extensive coastal areas worldwide, coastal erosion mitigation measures are implemented, aiming to make coastal areas resilient to the effects induced by coastal erosion and the anticipated climate change related to storms, flood events and sea level rise. Numerical modelling can support planned and sustainable coastal management from a medium-to-long-term perspective (decades). This research focuses on presenting contributions regarding the numerical modelling of subaerial beach dynamics (berm width and dune systems interactions) from a medium-term perspective. The method applied is based on a combination of the results of two simplified numerical models (the LTC and CS-Model). The results demonstrate the potential of the proposed combined model for medium-term projections, allowing for the interpretation of beach–dune dynamics and the evaluation of the importance of longshore and cross-shore sediment transport processes.
Multidisciplinary Design Optimization of Underwater Vehicles Based on a Combined Proxy Model
Authors: Sun Shaojun , Luo Weilin
Published: June 2024

To improve the efficiency of the multidisciplinary design optimization of underwater vehicles, this paper proposes a combined proxy model with adaptive dynamic sampling. The radial basis function model (RBF), Kriging model, and polynomial response surface model (PRS) are used to construct the proxy model. Efficient sample points are collected based on the synthetic minority oversampling technique (SMOTE) algorithm and the lower confidence bound (LCB) criterion. The proxy model process is integrated after dynamic sampling. The collaborative optimization framework is used, which considers the coupling between the main system set and the subsystem set. The hierarchical analysis method is used to transform the multidisciplinary optimization problem into a single-objective optimization problem. Computational fluid dynamics (CFD) numerical simulation is utilized to simulate underwater submarine navigation. The optimization strategy is applied to the underwater vehicle SUBOFF to optimize resistance and energy consumption. Three dynamic proxy models and three static proxy models are compared. The results show that the optimization efficiency of the underwater vehicle has been improved. To prove the generalization performance of the proposed combined proxy model, a reducer example is investigated for comparison. The results show that the combined proxy model (CPM) is highly accurate and has excellent generalization performance.
Risk Analysis of Pirate Attacks on Southeast Asian Ships Based on Bayesian Networks
Authors: Chen Qiong , Zhang Jinsheng , Gao Jiaqi , Lau Yui-Yip , Liu Jieming , Poo Mark Ching-Pong , Zhang Pengfei
Published: June 2024

As a bridge for international trade, maritime transportation security is crucial to the global economy. Southeast Asian waters have become a high-incidence area of global piracy attacks due to geographic location and complex security situations, posing a great threat to the development of the Maritime Silk Road. In this study, the factors affecting the risk of pirate attacks are analyzed in depth by using the Global Ship Piracy Attacks Report from the IMO Global Integrated Shipping Information System (GISIS) database (i.e., 2013–2022) in conjunction with a Bayesian Network (BN) model, and the Expectation Maximization algorithm is used to train the model parameters. The results show that piracy behaviors and the ship’s risk are the key factors affecting the risk of pirate attacks, and suggestions are made to reduce the risk of pirate attacks. This study develops a theoretical basis for preventing and controlling the risk of pirate attacks on ships, which helps maintain the safety of ship operations.
Systemic Symptoms as Potential Predictors of Chronic Neck Pain on Initial Examination: Can Systemic Symptoms Act as a Predictor of Neck Pain?
Authors: Park Seo-Hyun , Keum Dong-Ho
Published: June 2024

Background: Neck pain is a prevalent musculoskeletal disorder that can cause other additional misalignments and other misalignment-induced chronic musculoskeletal diseases. Although numerous risk factors for chronic neck pain have been researched, systemic symptoms have not received the same level of investigation. The aim of this study was to analyze the link between subjective systemic symptoms and neck pain based on initial holistic interviews, with the objective of identifying potential predictive factors for neck pain. Methods: This retrospective cross-sectional study included patients hospitalized due to acute neck pain between January 2018 and August 2021. Data collected included demographic information, treatment details, neck-pain characteristics, medical history, and co-occurring symptoms regardless of their known association with neck pain. Statistical analyses, including independent t-tests, Mann–Whitney U tests, chi-squared tests, Fisher’s exact tests, and correlation analyses, were performed. Results: With regard to the demographic characteristics, a significant positive correlation was observed between age and initial pain intensity (p < 0.01), while female sex was associated with changes in pain intensity (p < 0.05). Past medical conditions, including diabetes, hyperlipidemia, heart attacks, and psychological medical history, demonstrated a significant relationship with neck pain (p < 0.01, p < 0.05, p < 0.05, and p < 0.05, respectively). Hospitalization duration, cervical dizziness, limitations in the cervical range of motion (ROM), and widespread pain were significantly associated with neck pain (p < 0.05, p < 0.05, p < 0.01, and p < 0.001, respectively). Among the subjective systemic symptoms, only upper gastrointestinal (GI) disturbance displayed a significant association with neck pain (p < 0.01). Conclusions: This study identified several potential predictors of neck pain—notably, upper GI disturbances—providing a new avenue to investigate the prognostic factors of neck pain. However, further study is needed to substantiate these findings and elucidate the precise nature of these associations.
The Effect of Manner of Articulation and Syllable Affiliation on Tongue Configuration for Catalan Stop–Liquid and Liquid–Stop Sequences: An Ultrasound Study
Authors: Recasens Daniel
Published: June 2024

The present study reports tongue configuration data recorded with ultrasound for two sets of consonant sequences uttered by five native Catalan speakers. Articulatory data for the onset cluster pairs [kl]-[ɣl] and [kɾ]-[ɣɾ], and also for [l#k]-[l#ɣ] and [r#k]-[r#ɣ], analyzed in the first part of the investigation revealed that, as a general rule, the (shorter) velar approximant is less constricted than the (longer) voiceless velar stop at the velar and palatal zones while exhibiting a more retracted tongue body at the pharynx. These manner of articulation-dependent differences may extend into the preceding liquid. Data for [k#l]-[kl] and [k#r]-[kɾ] dealt with in the second part of the study show that the velar is articulated with more tongue body retraction for [k#l] vs. [kl] and for [k#r] vs. [kɾ], and with a higher tongue dorsum for [k#l] vs. [kl] and the reverse for [k#r] vs. [kɾ]. Therefore, clusters are produced with a more extreme lingual configuration across a word boundary than in syllable-onset position, which at least in part may be predicted by segmental factors for the [k#r]-[kɾ] pair. These articulatory data are compared with duration data for all sequence pairs.
On the Representation of Implicit Arguments in Child Grammar: Short Passives in Mandarin and English
Authors: Liu Minqi , Mateu Victoria , Hyams Nina
Published: June 2024

In this paper, we investigate whether Mandarin short passives contain an implicit external argument (EA; such as an agent or experiencer). Syntactic tests yield inconclusive results in Mandarin. We employ intervention effects as a diagnostic tool and demonstrate that, unlike English, Mandarin short passives do not syntactically project an implicit EA. Our findings are based on spontaneous language data from the CHILDES corpora, encompassing 1182 children aged 2–6 years, and experimental data from 78 Mandarin-speaking children aged 3–6 years. This cross-linguistic structural difference explains the earlier acquisition of short passives in Mandarin compared to English and also raises a learnability question, which we briefly address: how do children know to project an implicit EA or not in their language?