bluuebunny/crossref_metadata_2025 huggingface dataset:
Laminarin Reduces Cholesterol Uptake and NPC1L1 Protein Expression in High-Fat Diet (HFD)-Fed Mice
Authors: He Zhuoqian , Zhang Zhongyin , Xu Pengfei , Dirsch Verena M. , Wang Limei , Wang Kewei
Published: November 2023

Aberrantly high dietary cholesterol intake and intestinal cholesterol uptake lead to dyslipidemia, one of the risk factors for cardiovascular diseases (CVDs). Based on previous studies, laminarin, a polysaccharide found in brown algae, has hypolipidemic activity, but its underlying mechanism has not been elucidated. In this study, we investigated the effect of laminarin on intestinal cholesterol uptake in vitro, as well as the lipid and morphological parameters in an in vivo model of high-fat diet (HFD)-fed mice, and addressed the question of whether Niemann–Pick C1-like 1 protein (NPC1L1), a key transporter mediating dietary cholesterol uptake, is involved in the mechanistic action of laminarin. In in vitro studies, BODIPY-cholesterol-labeled Caco-2 cells were examined using confocal microscopy and a fluorescence reader. The results demonstrated that laminarin inhibited cholesterol uptake into Caco-2 cells in a concentration-dependent manner (EC50 = 20.69 μM). In HFD-fed C57BL/6J mice, laminarin significantly reduced the serum levels of total cholesterol (TC), total triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C). It also decreased hepatic levels of TC, TG, and total bile acids (TBA) while promoting the excretion of fecal cholesterol. Furthermore, laminarin significantly reduced local villous damage in the jejunum of HFD mice. Mechanistic studies revealed that laminarin significantly downregulated NPC1L1 protein expression in the jejunum of HFD-fed mice. The siRNA-mediated knockdown of NPC1L1 attenuated the laminarin-mediated inhibition of cholesterol uptake in Caco-2 cells. This study suggests that laminarin significantly improves dyslipidemia in HFD-fed mice, likely by reducing cholesterol uptake through a mechanism that involves the downregulation of NPC1L1 expression.
Sexual Violence and Alcohol Intake: A Population-Based Explorative Study in a Northwestern Italian Area
Authors: Mognetti Barbara , Scipio Federica Di , Abbadessa Giuliana , Carnino Giulia , Canavese Antonella , Castagna Paola , Romano Federica , Gino Sarah , Berta Giovanni N.
Published: November 2023

Background and Objectives: Sexual violence (SV) is a major global public health concern. While socioeconomic factors and familial relationships have been widely reported to contribute to SV, the role of alcohol consumption should not be ignored. Indeed, alcohol can impair cognition, distort reality, increase aggression, and ease drug-facilitated sexual assault. This retrospective study aims to explore the relationship between alcohol consumption and SV by examining the prevalence, characteristics, and consequences of violence episodes. Materials and Methods: A total of 1481 women accessed the Rape Centre “Centro Soccorso Violenza Sessuale” in Turin, Italy between 2008 and 2019, with 223 reporting alcohol consumption before the assault. Results: The alcohol group had a younger age profile, predominantly within the 18–25-year-old category. SV incidents involving alcohol consumers were more likely to occur in public places or in someone else’s home, while the non-alcohol-consuming group experienced more violence in their own homes. Acquaintances and unknown individuals were primarily responsible, whereas partners were the most common perpetrators of violence against non-alcohol-consuming women. Alcohol consumers sought medical attention sooner after the assault and exhibited more symptoms and injuries, particularly of neurological origin. Concurrent use of recreational drugs was higher among alcohol consumers. The logistic regression analysis revealed higher odds of injury for Italian women and those in the 18–35 age groups after consuming alcohol. Conclusions: This study contributes to the understanding of the relationship between alcohol consumption and SV. The prevalence of alcohol-related sexual aggression is lower compared to that shown in previous studies. Nationality, age, and assailant identity influence SV dynamics. These findings can guide well-targeted interventions and prevention strategies to address SV and inform communities facing similar challenges.
A Low Geriatric Nutritional Risk Index Is Associated with Low Muscle Volume and a Poor Prognosis among Cirrhotic Patients
Authors: Enomoto Hirayuki , Yuri Yukihisa , Nishimura Takashi , Ikeda Naoto , Takashima Tomoyuki , Aizawa Nobuhiro , Okamoto Mamiko , Yoshihara Kohei , Yoshioka Ryota , Kawata Shoki , Kawase Yuta , Nakano Ryota , Shiomi Hideyuki , Fukunishi Shinya , Shinzaki Shinichiro , Iijima Hiroko
Published: November 2023

Background and Objectives: The geriatric nutritional risk index (GNRI) is an easily calculable index that can be determined using three common clinical variables. The GNRI is suggested to be related to sarcopenia in cirrhotic patients. However, the relationship between the GNRI and the prognosis in patients with liver cirrhosis (LC) has not been reported. The aim of the present research is to study the association of the GNRI with the nutritional status, hepatic function reserve, and prognosis in patients with liver cirrhosis (LC). Materials and Methods: A total of 370 cirrhotic patients whose nutritional statuses were evaluated using anthropometric measurements and bioimpedance analysis were studied. The associations between the GNRI and nutritional status and the GNRI and hepatic function reserve were analyzed. We also investigated the GNRI and prognosis of patients with LC. Results: The median age of the enrolled patients was 66 years old, and 266 (71.9%) patients had viral hepatitis-related LC. The GNRI was shown to decrease with the progression of chronic liver disease, represented by an increased FIB-4 index and severe Child–Pugh and mALBI grades. In addition, a low GNRI (<92) was associated with severe cirrhosis-related metabolic disorders, including a low branched-chain amino acid-to-tyrosine ratio (BTR) and a low zinc value. The GNRI was positively correlated with two nutrition-related anthropometric variables (% arm circumference and % arm muscle circumference), and a low GNRI was related to a low skeletal muscle mass index (SMI) (<7.0 kg/m2 for men or <5.7 kg/m2 for women), as determined by using bioimpedance analysis. In addition, patients with a low GNRI (<92) had a poorer prognosis than those with a high GNRI (≥92) (log-rank test: p = 0.0161, and generalized Wilcoxon test, p = 0.01261). Conclusions: Our results suggest that a low GNRI is related to severe chronic liver disease, low muscle volume, and a poor prognosis of patients with cirrhosis.
Numerical Simulation of Membrane Separation Characteristics of Supercritical Carbon Dioxide and Water
Authors: Qiao Zongliang , Pan Yue , Tang Youfei , Cao Yue , Si Fengqi
Published: November 2023

To solve the problem of water carryover in the supercritical CO2 separation and mining process in the CO2 plume geothermal system, a three-dimensional shell-tube hollow fiber membrane absorption separator is designed in this study. A coupled species transport model, a porous medium model, and an absorption mathematical model are established, and the flow field and separation characteristics in the circular and flat tubes are analyzed using numerical simulation. The results show that the membrane separation efficiency increases with an increase in the flatness and membrane tube length. When the inlet velocity of the mixture is 0.1 m/s, the separation efficiency can reach 75.92%. Selecting a smaller flow Reynolds number and a more significant membrane tube flatness will reduce the water mass fraction at the outlet. When adding baffles of different shapes to the membrane tube, the mixture fluid in the membrane tube meanders forward and flows in the shape of “Z” under the blocking effect of the arcuate baffles. With an increase in the number of arcuate baffles in the membrane tube, the membrane separation efficiency of the separator increases continuously. The mixture fluid flows in the membrane tube with the built-in torsional baffles in a spiral manner, and the separation efficiency of the membrane separator increases with a torsion ratio reduction in the membrane tube.
Dual Coupled Long-Range Hybrid Surface Plasmon Polariton Waveguide for Sub-Wavelength Confinement
Authors: Wang Yindi , Wang Shulong , Zhao Juanning , Xue Mingyuan
Published: November 2023

In this paper, a long-range hybrid waveguide for subwavelength confinement based on double SPP coupling is proposed. The hybrid waveguide consists of a metal-based cylindrical hybrid waveguide and a silver nanowire. There are two coupling regions in the waveguide structure that enhance mode coupling. Strong mode coupling enables the waveguide to exhibit both a small effective mode area (0.01) and an extremely long transmission length (700 μm). The figure of merit (FOM) of the waveguide can be as high as 4000. In addition, the cross-sectional area of the waveguide is only 500 nm × 500 nm, allowing optical operation in the subwavelength range, which helps enhance the miniaturization of optoelectronic devices. The excellent characteristics of the hybrid waveguide make it have potential applications in photoelectric integrated systems.
The Influence of Laser Process Parameters on the Adhesion Strength between Electroless Copper and Carbon Fiber Composites Determined Using Response Surface Methodology
Authors: Wang Xizhao , Liu Jianguo , Liu Haixing , Zhou Zhicheng , Qin Zhongli , Cao Jiawen
Published: November 2023

Laser process technology provides a feasible method for directly manufacturing surface-metallized carbon fiber composites (CFCs); however, the laser’s process parameters strongly influence on the adhesion strength between electroless copper and CFCs. Here, a nanosecond ultraviolet laser was used to fabricate electroless copper on the surface of CFCs. In order to achieve good adhesion strength, four key process parameters, namely, the laser power, scanning line interval, scanning speed, and pulse frequency, were optimized experimentally using response surface methodology, and a central composite design was utilized to design the experiments. An analysis of variance was conducted to evaluate the adequacy and significance of the developed regression model. Also, the effect of the process parameters on the adhesion strength was determined. The numerical analysis indicated that the optimized laser power, scanning line interval, scanning speed, and pulse frequency were 5.5 W, 48.2 μm, 834.0 mm/s, and 69.5 kHz, respectively. A validation test confirmed that the predicted results were consistent with the actual values; thus, the developed mathematical model can adequately predict responses within the limits of the laser process parameters being used.
Investigation of a Circularly Polarized Metasurface Antenna for Hybrid Wireless Applications
Authors: Behera Bikash Ranjan , Alsharif Mohammed H. , Jahid Abu
Published: November 2023

The increasing prevalence of the Internet of Things (IoT) as the primary networking infrastructure in a future society, driven by a strong focus on sustainability and data, is noteworthy. A significant concern associated with the widespread use of Internet of Things (IoT) devices is the insufficient availability of viable strategies for effectively sustaining their power supply and ensuring their uninterrupted functionality. The ability of RF energy-harvesting systems to externally replenish batteries serves as a primary driver for the development of these technologies. To effectively mitigate concerns related to wireless technology, it is imperative to adhere strictly to the mandated limitations on electromagnetic field emissions. A TA broadband polarization-reconfigurable Y-shaped monopole antenna that is improved with a SADEA-tuned smart metasurface is one technique that has been proposed in order to accomplish this goal. A Y-shaped printed monopole antenna is first taken into consideration. To comprehend the process of polarization reconfigurability transitioning from linear to circular polarization (CP), a BAR 50-02 V RF PIN Diode is employed to shorten one of the parasitic conducting strips to the ground plane. A SADEA-driven metasurface, which utilizes the artificial intelligence-driven surrogate model-assisted differential evolution for antenna synthesis, is devised and positioned beneath the radiator to optimize performance trade-offs while increasing the antenna’s gain and bandwidth. The ultimate prototype achieves the following: an impedance bandwidth of 2.58 GHz (3.27–5.85 GHz, 48.45%); an axial bandwidth of 1.25 GHz (4.19–5.44 GHz, 25.96%); a peak gain exceeding 8.45 dBic; and when a highly efficient rectifier is integrated, the maximum RF-DC conversion efficiency of 73.82% and DC output of 5.44 V are obtained. Based on the results mentioned earlier, it is considered appropriate to supply power to intelligent sensors and reduce reliance on batteries via RF energy-harvesting mechanisms implemented in hybrid wireless applications.
Fabrication and Applications of Magnetic Polymer Composites for Soft Robotics
Authors: Ganguly Sayan , Margel Shlomo
Published: November 2023

The emergence of magnetic polymer composites has had a transformative impact on the field of soft robotics. This overview will examine the various methods by which innovative materials can be synthesized and utilized. The advancement of soft robotic systems has been significantly enhanced by the utilization of magnetic polymer composites, which amalgamate the pliability of polymers with the reactivity of magnetic materials. This study extensively examines the production methodologies involved in dispersing magnetic particles within polymer matrices and controlling their spatial distribution. The objective is to gain insights into the strategies required to attain the desired mechanical and magnetic properties. Additionally, this study delves into the potential applications of these composites in the field of soft robotics, encompassing various devices such as soft actuators, grippers, and wearable gadgets. The study emphasizes the transformative capabilities of magnetic polymer composites, which offer a novel framework for the advancement of biocompatible, versatile soft robotic systems that utilize magnetic actuation.
Research on Geometric Constraint Strategies for Controlling the Diameter of Micro-Shafts Manufactured via Wire Electric Discharge Grinding
Authors: Jia Jianyu , Li Zan , Hu Bo , Wang Yanqing , Wang Jing , Li Congbo , Xiang Wenfeng
Published: November 2023

Micro-tools comprising difficult-to-machine materials have seen widespread application in micro-manufacturing to satisfy the demands of micro-part processing and micro-device development. Taking micro-shafts as an example, the related developmental technology, based on wire electric discharge grinding (WEDG) as the core method, is one of the key technologies used to prepare high-precision micro-shafts. To enable efficient and high-precision machining of micro-shafts with target diameters, instead of performing multiple repeated on-machine measurements and reprocessing, a geometric constraint strategy is proposed based on the previously introduced twin-mirroring-wire tangential feed electrical discharge grinding (TMTF-WEDG). This strategy encompasses the tool setting method, tangential feed distance compensation, and an equation that establishes the relationship between tangential distance and diameter variation. These components are derived from a key points analysis of the geometric constraints. The micro-shafts with diameters of 50 µm and consistencies of ±1.5 µm are repeatedly processed. A series of micro-shafts with diameters ranging from 30 µm to 120 µm achieve geometric constraints with a diameter accuracy of ±2 µm, accompanied by the complete continuous automation of the entire process. Accordingly, it can be concluded that the geometric constraint strategy is flexible and stable and can be controlled with high precision in the TMTF-WEDG process.
A High-Precision Voltage-Quantization-Based Current-Mode Computing-in-Memory SRAM
Authors: Zhao Ruiyong , Gong Zhenghui , Liu Yulan , Chen Jing
Published: November 2023

Non-linear distortion of signals is a serious problem in computing-in-memory SRAM (CIM-SRAM) circuits in current mode. This problem greatly limits the performance of calculations and directly affects the computing power of the CIM-SRAM. In this study, the causes of non-linearity and inconsistency were investigated. Based on detailed analyses, we proposed a high-precision, fully dynamic range IV (HFIV) conversion circuit. The HFIV circuit was added to each bit line (BL) for voltage clamping and proportionally mirroring the read current. We applied the structure to numerous prior studies and evaluated them using the 55 nm complementary metal-oxide semiconductor process. The results showed the proposed HFIV circuit could increase the CIM-SRAM’s calculation linearity to 99.92% (8~32 SRAM bit-cells) and 99.8% (32~64 SRAM bit-cells) with a 1.2 V supply.
Evaluation of a Method for Standardized Antimicrobial Susceptibility Testing with Mycoplasma hyorhinis Field Isolates
Authors: Käbisch Lisa , Schink Anne-Kathrin , Hoeltig Doris , Verspohl Jutta , Gyuranecz Miklós , Spergser Joachim , Kehrenberg Corinna , Schwarz Stefan
Published: November 2023

Organizations like the Clinical and Laboratory Standards Institute (CLSI) or the European Committee of Antimicrobial Susceptibility Testing (EUCAST) provide standardized methodologies for antimicrobial susceptibility testing of a wide range of nonfastidious and fastidious bacteria, but so far not for Mycoplasma spp. of animal origin. Recently, a proposed method for the standardized broth microdilution testing of Mycoplasma hyorhinis using commercial Sensititre microtiter plates was presented. In this study, we evaluated this broth microdilution method with 37 field isolates and tested their susceptibility toward the following antimicrobial agents: doxycycline, enrofloxacin, erythromycin, florfenicol, gentamicin, marbofloxacin, tetracycline, tiamulin, tilmicosin, tulathromycin, and tylosin. The isolates originated from different countries, isolation sites, and years. The broth microdilution method was carried out using a modified Friis broth as the culture and test medium. For macrolides and lincosamides, a bimodal distribution with elevated MIC values could be observed for almost half of the tested field isolates, deducing reduced susceptibility toward these substances. With a recently published protocol, we were able to test a variety of field isolates, and consistent data could be obtained. Using this method, monitoring studies of Mycoplasma hyorhinis isolates can be carried out in a comparable manner, and the observed susceptibility profiles can be screened for possible changes in MIC values in the future.
Evaluation of Bacterial and Fungal Biomarkers for Differentiation and Prognosis of Patients with Inflammatory Bowel Disease
Authors: Yoon Hyuk , Park Sunghyouk , Jun Yu Kyung , Choi Yonghoon , Shin Cheol Min , Park Young Soo , Kim Nayoung , Lee Dong Ho
Published: November 2023

This study aimed to evaluate bacterial and fungal biomarkers to differentiate patients with inflammatory bowel disease (IBD), predict the IBD prognosis, and determine the relationship of these biomarkers with IBD pathogenesis. The composition and function of bacteria and fungi in stool from 100 IBD patients and 97 controls were profiled using next-generation sequencing. We evaluated the cumulative risk of relapse according to bacterial and fungal enterotypes. The microbiome and mycobiome alpha diversity in IBD patients were significantly lower and higher than in the controls, respectively; the micro/mycobiome beta diversity differed significantly between IBD patients and the controls. Ruminococcus gnavus, Cyberlindnera jadinii, and Candida tropicalis increased in IBD patients. Combining functional and species analyses revealed that lower sugar import and higher modified polysaccharide production were associated with IBD pathogenesis. Tricarboxylic acid cycling consuming acetyl CoA was higher in IBD patients than the controls, leading to lower short-chain fatty acid (SCFA) fermentation. Bacterial and fungal enterotypes were not associated with IBD relapse. We found differences in bacterial and fungal species between IBD patients and controls. A working model for the role of gut bacteria in IBD pathogenesis is proposed, wherein bacterial species increase modified N-glycan production and decrease SCFA fermentation.
Cationic Surfactant-Modified Tetraselmis sp. for the Removal of Organic Dyes from Aqueous Solution
Authors: Buhani  , Istikomah  , Suharso  , Sumadi  , Sutarto  , Alghamdi Huda M. , Elwakeel Khalid Z.
Published: November 2023

The modification of the Tetraselmis sp. algae material (Tetra-Alg) with surfactant Cethyltrimethylammonium Bromide (CTAB) yielded adsorbent Tetra-Alg-CTAB as an adsorbent of methyl orange (MO) and methylene blue (MB) solutions. The characterization of the adsorbent used an infrared (IR) spectrometer to identify functional groups and Scanning Electron Microscopy with Energy Dispersive X-ray (SEM-EDX FEI Inspect-S50, Midland, ON, Canada) to determine the surface morphology and elemental composition. Methyl orange and methylene blue adsorption on the adsorbent Tetra-Alg, Tetraselmis sp. algae-modified Na+ ions (Tetra-Alg-Na), and Tetra-Alg-CTAB were studied, including variations in pH, contact time, concentration, and reuse of adsorbents. The adsorption of MO and MB by Tetra-Alg-CTAB at pH 10, during a contact time of 90 min, and at a concentration of 250 mg L−1 resulted in MO and MB being absorbed in the amounts of 128.369 and 51.013 mg g−1, respectively. The adsorption kinetics and adsorption isotherms of MO and MB and Tetra-Alg, Tetra-Alg-Na, and Tetra-Alg-CTAB tend to follow pseudo-second-order kinetics models and Freundlich adsorption isotherms with each correlation coefficient value (R2) approaching 1. Due to the modification with the cationic surfactant CTAB, anionic dyes can be strongly sorbed in alkaline pH due to strong electrostatic attraction, while MB is more likely to involve cation exchange and hydrogen bonding. The reuse of Tetra-Alg-CTAB was carried out four times with adsorption percent > 70%, and the adsorbent was very effective in the adsorption of anionic dyes such as MO.
Salazinic Acid and Norlobaridone from the Lichen Hypotrachyna cirrhata: Antioxidant Activity, α-Glucosidase Inhibitory and Molecular Docking Studies
Authors: Kumar Tatapudi Kiran , Siva Bandi , Kiranmai Basani , Alli Vidya Jyothi , Jadav Surender Singh , Reddy Araveeti Madhusudana , Boustie Joël , Le Devehat Françoise , Tiwari Ashok Kumar , Suresh Babu Katragadda
Published: November 2023

The present study was intended for the identification of secondary metabolites in acetone extract of the lichen Hypotrachyna cirrhata using UPLC-ESI-QToF-MS/MS and the detection of bioactive compounds. This study led to the identification of 22 metabolites based on their MS/MS spectra, accurate molecular masses, molecular formula from a comparison of the literature database (DNP), and fragmentation patterns. In addition, potent antioxidant and α-glucosidase inhibitory potentials of acetone extract of H. cirrhata motivated us to isolate 10 metabolites, which were characterized as salazinic acid (11), norlobaridone (12), atranorin (13), lecanoric acid (14), lichesterinic acid (15), protolichesterinic acid (16), methyl hematommate (17), iso-rhizonic acid (18), atranol (19), and methylatratate (20) based on their spectral data. All these isolates were assessed for their free radicals scavenging, radical-induced DNA damage, and intestinal α-glucosidase inhibitory activities. The results indicated that norlobaridone (12), lecanoric acid (14), methyl hematommate (17), and atranol (19) showed potent antioxidant activity, while depsidones (salazinic acid (11), norlobaridone (12)) and a monophenolic compound (iso-rhizonic acid, (18)) displayed significant intestinal α-glucosidase inhibitory activities (p < 0.001), which is comparable to standard acarbose. These results were further correlated with molecular docking studies, which indicated that the alkyl chain of norlobaridione (12) is hooked into the finger-like cavity of the allosteric pocket; moreover, it also established Van der Waals interactions with hydrophobic residues of the allosteric pocket. Thus, the potency of norlobaridone to inhibit α-glucosidase enzyme might be associated with its allosteric binding. Also, MM-GBSA (Molecular Mechanics-Generalized Born Surface Area) binding free energies of salazinic acid (11) and norlobaridone (12) were superior to acarbose and may have contributed to their high activity compared to acarbose.
Electrically Induced Structural Transformations of a Chiral Nematic under Tangential-Conical Boundary Conditions
Authors: Kostikov Denis A. , Krakhalev Mikhail N. , Prishchepa Oxana O. , Zyryanov Victor Ya.
Published: November 2023

In this study, structural transformations induced by an electric field in the chiral nematic under tangential-conical boundary conditions have been considered. The composition influence of the orienting polymer films on the director tilt angles, the formation of orientational structures in the LC layer, as well as the electro-optical response and relaxation processes have been studied. It has been shown that the poly(tert-butyl methacrylate) concentration change in the orienting polymer mixture allows for smoothly controlling the director tilt angle without fixing its azimuthal orientation rigidly.
Preparation of Thin Films Containing Modified Hydroxyapatite Particles and Phospholipids (DPPC) for Improved Properties of Biomaterials
Authors: Rojewska Monika , Adamska Katarzyna , Kurnatowska Justyna , Miklaszewski Andrzej , Bartkowska Aneta , Prochaska Krystyna
Published: November 2023

The main aims of thin biofilm synthesis are to either achieve a new form to promote the transport of drugs in oral delivery systems or as a coating to improve the biocompatibility of the implant’s surface. In this study, the Langmuir monolayer technique was employed to obtain films containing Mg-doped hydroxyapatite with 0.5%, 1.0%, and 1.5% Mg(II). The obtained modified HA particles were analysed via the FT-IR, XRD, DLS, and SEM methods. It was shown that the modified hydroxyapatite particles were able to form thin films at the air/water interface. BAM microscopy was employed to characterized the morphology of these films. In the next step, the mixed films were prepared using phospholipid (DPPC) molecules and modified hydroxyapatite particles (HA-Mg(II)). We expected that the presence of phospholipids (DPPC) in thin films improved the biocompatibility of the preparing films, while adding HA-Mg(II) particles will promote antibacterial properties and enhance osteogenesis processes. The films were prepared in two ways: (1) by mixing DPPC and HA-Mg (II) and spreading this solution onto the subphase, or (2) by forming DPPC films, dropping the HA-Mg (II) dispersion onto the phospholipid monolayer. Based on the obtained π–A isotherms, the surface parameters of the achieved thin films were estimated. It was observed that the HA-Mg(II) films can be stabilized with phospholipid molecules, and a more stable structure was obtained from films synthesied via method (2).
Single-Step Formation of Metal Oxide Nanostructures Wrapped in Mesoporous Silica and Silica–Niobia Catalysts for the Condensation of Furfural with Acetone
Authors: Skrodczky Kai , Antunes Margarida M. , Zhu Qingjun , Valente Anabela A. , Pinna Nicola , Russo Patrícia A.
Published: November 2023

The integration of metal oxide nanomaterials with mesoporous silica is a promising approach to exploiting the advantages of both types of materials. Traditional synthesis methods typically require multiple steps. This work instead presents a fast, one-step, template-free method for the synthesis of metal oxides homogeneously dispersed within mesoporous silica, including oxides of W, Ti, Nb, Ta, Sn, and Mo. These composites have tunable metal oxide contents, large surface areas, and wide mesopores. The combination of Nb2O5 nanoparticles (NPs) with SiO2 results in an increased surface area and a larger number of acid sites compared to pure Nb2O5 NPs. The surface texture and acidity of the silica–niobia composites can be tuned by adjusting the Nb/Si molar ratio. Moreover, the silica provides protection to the niobia NPs, preventing sintering during thermal treatment at 400 °C. The silica–niobia materials exhibit superior performance as catalysts in the aldol condensation of furfural (Fur) with acetone compared to pure niobia, leading to an up to 62% in product yield. Additionally, these catalysts show remarkable stability, retaining their performance over multiple runs. This work demonstrates the potential of the proposed synthesis approach for preparing more sustainable, high-performance, durable, and stable nanoscale metal oxide-based catalysts with a tunable composition, surface area, and active site density.
Excitons and Phonons in Two-Dimensional Materials: From Fundamental to Applications
Authors: Molas Maciej R.
Published: November 2023

The isolation of graphene opened the gate to investigate a vast family of two-dimensional (2D) layered materials [...]
Symmetry-Engineering-Induced In-Plane Polarization Enhancement in Ta2NiS5/CrOCl van der Waals Heterostructure
Authors: Su Yue , Chen Peng , Xu Xiangrui , Zhang Yufeng , Cai Weiwei , Peng Gang , Zhang Xueao , Deng Chuyun
Published: November 2023

Van der Waals (vdW) interfaces can be formed via layer stacking regardless of the lattice constant or symmetry of the individual building blocks. Herein, we constructed a vdW interface of layered Ta2NiS5 and CrOCl, which exhibited remarkably enhanced in-plane anisotropy via polarized Raman spectroscopy and electrical transport measurements. Compared with pristine Ta2NiS5, the anisotropy ratio of the Raman intensities for the B2g, 2Ag, and 3Ag modes increased in the heterostructure. More importantly, the anisotropy ratios of conductivity and mobility in the heterostructure increased by one order of magnitude. Specifically speaking, the conductivity ratio changed from ~2.1 (Ta2NiS5) to ~15 (Ta2NiS5/CrOCl), while the mobility ratio changed from ~2.7 (Ta2NiS5) to ~32 (Ta2NiS5/CrOCl). Such prominent enhancement may be attributed to the symmetry reduction caused by lattice mismatch at the heterostructure interface and the introduction of strain into the Ta2NiS5. Our research provides a new perspective for enhancing artificial anisotropy physics and offers feasible guidance for future functionalized electronic devices.
The Association between Glucose 6-Phosphate Dehydrogenase Deficiency and Attention Deficit/Hyperactivity Disorder
Authors: Merzon Eugene , Magen Eli , Ashkenazi Shai , Weizman Abraham , Manor Iris , Krone Beth , Green Ilan , Golan-Cohen Avivit , Vinker Shlomo , Faraone Stephen V. , Israel Ariel
Published: November 2023

Background: Glucose-6-phosphate dehydrogenase (G6PD) deficiency, impacting 4.9% of the population and more prevalent in Mediterranean communities, is a common enzymopathy with potential relevance to Attention Deficit/Hyperactivity Disorder (ADHD). This study investigated this association. Methods: The clinical characteristics of 7473 G6PD-deficient patients and 29,892 matched case–controls (selected at a 1:4 ratio) from a cohort of 1,031,354 within the Leumit Health Services database were analyzed using Fisher’s exact test for categorical variables and the Mann–Whitney U test for continuous variables. Results: In total, 68.7% were male. The mean duration of follow-up was 14.3 ± 6.2 years at a mean age of 29.2 ± 22.3 years. G6PD deficiency was associated with an increased risk of being diagnosed with ADHD (Odds Ratio (OR) = 1.16 [95% CI, 1.08–1.25], p < 0.001), seeking care from adult neurologists (OR = 1.30 [95% CI, 1.22–1.38], p < 0.001), and consulting adult psychiatrists (OR = 1.12 [95% CI, 1.01–1.24], p = 0.048). The use of stimulant medications among G6PD-deficient individuals was 17% higher for the methylphenidate class of drugs (OR = 1.17 [95% CI, 1.08, 1.27], p < 0.001), and there was a 16% elevated risk for amphetamine use (OR = 1.16 [95% CI, 1.03, 1.37], p = 0.047). Conclusions: G6PD deficiency signals an increased risk of ADHD diagnosis, more severe presentations of ADHD and a greater need for psychiatric medications to treat ADHD.
Metabolomic Alterations of Volatile Organic Compounds and Bile Acids as Biomarkers of Microbial Shifts in a Murine Model of Short Bowel Syndrome
Authors: Wolfschluckner Vanessa , Obermüller Beate , Horvath Angela , Rodriguez-Blanco Giovanny , Fuchs Patricia , Miekisch Wolfram , Mittl Barbara , Flucher Christina , Till Holger , Singer Georg
Published: November 2023

Pediatric short bowel syndrome (SBS) is a rare condition characterized by a massive loss of the small intestine, leading to the inability to meet nutritional requirements without the use of parenteral or enteral supplementation. SBS causes profound alterations in the intestinal microbiome and metabolome. The aim of this study was a detailed assessment of the intestinal microbiome and metabolome in a murine model of SBS. We performed a 60% proximal small bowel resection versus a sham operation in C57BL/6 mice. Four weeks postoperatively, the microbial communities of different intestinal segments (jejunum, ileum, colon) and stool were assessed by 16S rRNA gene sequencing. Bile acids in serum and stool and volatile organic compounds (VOCs) in the fecal headspace were assessed using LC-MS and GC-MS techniques. The α-diversity of the different intestinal segments did not significantly differ between the two groups. β-diversity significantly differed between sham and SBS mice. While in the jejunum, Faecalibaculum was significantly increased in SBS animals, a significant reduction in Lactobacillus and Sporosarcina was detected in the ileum of SBS mice. In the colon of SBS mice, a significant decrease in Ruminococcaceae and a significant increase in Proteobacteria such as Faecalibaculum and Escherichia-Shigella were found. Serum levels of deoxycholic, taurocholic and taurochenodeoxycholic acids were significantly higher in the SBS group. Of the 29 VOCs tested, hexane, isoflurane and pentane were significantly higher in the SBS group, and pyrrole was significantly lower. We were able to show that SBS causes shifts in the murine intestinal microbiome and metabolome including serum BAs and fecal VOCs.
Safety of Innovative Nanotechnology Oral Formulations Loaded with Bioactive Menopause Molecules: Influence of Genotoxicity and Biochemical Parameters on a Menopausal Rat Model
Authors: Crișan Simona , Pop Anca Lucia , Lacatusu Ioana , Badea Nicoleta , Mustaciosu Cosmin , Radu Mihai , Varlas Valentin Nicolae , Peneş Ovidiu Nicolae , Ciobanu Anne Marie , Ghica Manuela , Voicu Sorina Nicoleta , Udeanu Denisa Ioana
Published: November 2023

In recent years, nanoparticles have gained significant importance due to their unique properties, such as pharmacological, electrical, optical, and magnetic abilities, contributing to the growth of the science and technology sector. Particular naturally derived biomolecules with beneficial effects on menopause disorder have been the subject of studies of pharmaceutical formulation to obtain alternative pharmaceutical forms with increased bioavailability and without side effects, as in nanostructured lipid carriers (NLCs) loaded with such active ingredients. In the present study, one stage of a broader project, we have performed pharmacotoxicology studies for six combinatory innovative nanocapsule pharmaceutical forms containing active natural biomolecules before considering them as oral formulas for (1) in vitro toxicity studies on culture cells and (2) in vivo preclinical studies on a surgically induced menopause model of Wistar female rats, and the influence of the NLCs on key biochemical parameters: lipid profile (TG, Chol, HDL), glycemic markers (Gli), bone markers (Pac, Palc, Ca, phosphorus), renal markers (Crea, urea, URAC), inflammation (TNF), oxidative stress (GSH, MDA), and estrogen–progesterone hormonal profile. The micronucleus test did not reveal the genotoxicity of the tested compounds; the menopause model showed no significant safety concerns for the six tested formulas evaluated using the blood biochemical parameters; and the results showed the potential hypoglycemic, hypolipidemic, hypouricemic, and antioxidant potential of one of the tested formulas containing nano diosgenin and glycyrrhizic acid.
Comment on Lopes et al. Adiposity Metabolic Consequences for Adolescent Bone Health. Nutrients 2022, 14, 3260
Authors: Boucher Barbara Joan
Published: November 2023

This interesting report [...]
The Impact of Clinical Factors, Vitamin B12 and Total Cholesterol on Severity of Anorexia Nervosa: A Multicentric Cross-Sectional Study
Authors: Affaticati Letizia Maria , Buoli Massimiliano , Vaccaro Nadia , Manzo Francesca , Scalia Alberto , Coloccini Sara , Zuliani Tommaso , La Tegola Davide , Capuzzi Enrico , Nicastro Monica , Colmegna Fabrizia , Clerici Massimo , Dakanalis Antonios , Caldiroli Alice
Published: November 2023

Severe forms of Anorexia Nervosa (AN) are characterized by medical complications, psychiatric comorbidity, and high mortality. This study investigated potential associations between clinical/biological factors and the severity of AN, measured by the Body Mass Index (BMI). Red and white blood cells, hemoglobin, platelets, iron, vitamins D and B12, folate, and total cholesterol were measured in a mixed sample of 78 inpatients and outpatients. Linear regressions and one-way analyses of variance (ANOVAs) were carried out to evaluate the relationship between BMI and clinical/biochemical variables. BMI was significantly lower in hospitalized patients (F = 4.662; p = 0.034) and in those under pharmacological treatment (F = 5.733; p = 0.019) or poly-therapy (F = 5.635; p = 0.021). Higher vitamin B12 (β = −0.556, p < 0.001), total cholesterol (β = −0.320, p = 0.027), and later age at onset (with a trend towards significance) (β = −0.376, p = 0.058) were associated with a lower BMI. Increased total cholesterol and vitamin B12, later age at onset, current pharmacological treatment, and poly-therapy might be distinctive in patients with a lower BMI. In clinical practice, these findings may contribute to the early identification of AN patients at higher risk of developing complicated or chronic forms of the disorder. Further studies on larger samples are needed to identify potential predictive factors of AN severity in the framework of precision medicine.
A Scoping Review to Explore the Potential Benefits of Nutrition Interventions for Latino/a Adult Cancer Survivors in the US
Authors: Johnson Cassandra M. , Stubblefield Emily , Godinich Brandon M. , Walker Miranda , Salcedo Price Ramona , Allicock Marlyn A.
Published: November 2023

Despite evidence for the role of healthy diets in preventing cancer, little is known about how nutrition can support positive health outcomes after a cancer diagnosis for Latino/a cancer survivors in the United States (U.S.). The purpose of this scoping review is to understand the potential benefits of nutrition interventions in supporting healthy survivorship among Latino/a cancer survivors in the U.S. A team compiled, evaluated, and summarized the available evidence. Potentially relevant studies were identified from a comprehensive search of peer-reviewed databases and the gray literature. Eligible studies included Latino/a adult cancer survivors with a nutrition education, dietary change, or behavioral intervention; and a nutrition-related health outcome. Data were extracted and summarized using tables. The review included 10 randomized controlled trials, with samples or subsamples of Latino/a cancer survivors. Interventions mostly focused on breast cancer survivors. The results showed some evidence that dietary behaviors, like fruit and vegetable intake, were related to positive outcomes, like a decreased risk of cancer (through changes in DNA methylation), decreased risk breast cancer recurrence (through changes in inflammatory biomarkers), or improved perception of health status. The findings highlight a need for community-engaged and culturally relevant nutrition interventions for Latino/a adults, especially for rural communities; and innovative intervention approaches, including m/ehealth approaches with long-term follow-up.
The Role of Cdc42 in the Insulin and Leptin Pathways Contributing to the Development of Age-Related Obesity
Authors: Umbayev Bauyrzhan , Saliev Timur , Safarova (Yantsen) Yuliya , Yermekova Aislu , Olzhayev Farkhad , Bulanin Denis , Tsoy Andrey , Askarova Sholpan
Published: November 2023

Age-related obesity significantly increases the risk of chronic diseases such as type 2 diabetes, cardiovascular diseases, hypertension, and certain cancers. The insulin–leptin axis is crucial in understanding metabolic disturbances associated with age-related obesity. Rho GTPase Cdc42 is a member of the Rho family of GTPases that participates in many cellular processes including, but not limited to, regulation of actin cytoskeleton, vesicle trafficking, cell polarity, morphology, proliferation, motility, and migration. Cdc42 functions as an integral part of regulating insulin secretion and aging. Some novel roles for Cdc42 have also been recently identified in maintaining glucose metabolism, where Cdc42 is involved in controlling blood glucose levels in metabolically active tissues, including skeletal muscle, adipose tissue, pancreas, etc., which puts this protein in line with other critical regulators of glucose metabolism. Importantly, Cdc42 plays a vital role in cellular processes associated with the insulin and leptin signaling pathways, which are integral elements involved in obesity development if misregulated. Additionally, a change in Cdc42 activity may affect senescence, thus contributing to disorders associated with aging. This review explores the complex relationships among age-associated obesity, the insulin–leptin axis, and the Cdc42 signaling pathway. This article sheds light on the vast molecular web that supports metabolic dysregulation in aging people. In addition, it also discusses the potential therapeutic implications of the Cdc42 pathway to mitigate obesity since some new data suggest that inhibition of Cdc42 using antidiabetic drugs or antioxidants may promote weight loss in overweight or obese patients.
Losses in the Care Cascade for Latent Tuberculosis Infection in the Tracing Contact Studies
Authors: Godoy Sofia , Parrón Ignasi , Millet Joan-Pau , Caylà Joan A. , Follia Núria , Carol Mònica , Orcau Àngels , Alsedà Miquel , Toledo Diana , Ferrús Glòria , Plans Pere , Barrabeig Irene , Clotet Laura , Domínguez Àngela , Godoy Pere ,   the Transmission of Tuberculosis in Catalonia (Spain) Working Group
Published: November 2023

Background: The control of latent tuberculosis infection (LTBI) encompasses multiple stages. The objective was to calculate the losses in the LTBI care cascade for pulmonary TB contacts in Catalonia (Spain). Methods: The LTBI care cascade was studied for pulmonary TB contacts reported from 1 January 2019 to 30 June 2021, considering three dependent variables: non-performance of testing; non-receipt of a treatment prescription; and non-adherence to treatment. Variables associated with the cascade were analysed using adjusted OR (aOR) and 95% confidence intervals (CI). Results: Identified from 847 cases of pulmonary TB were 7087 contacts, of whom 6537 (92.2%) could be screened for LTBI. LTBI prevalence was 25.5% (1670/6537); 69.4% of persons with LTBI (1159/1670) received a treatment prescription and 71.3% (827/1159) completed it. Treatment prescription was associated with age ≥65 years (aOR = 0.3; 95%CI: 0.2–0.6) and a daily exposure of ≥6 h to the TB index case (aOR = 3.6; 95%CI: 2.6–5.0). Treatment adherence was lower in men (aOR = 0.7; 95%CI: 0.5–1.0) and immigrants (aOR = 0.7; 95%CI: 0.5–0.9). Conclusions: Under 50% of contacts make it to the end of the LTBI cascade. Losses need to be reduced through education of both healthcare providers and patients and through treatment monitoring. The greater involvement of primary care physicians could help in monitoring and controling LTBI.
Exogenous Klotho Extends Survival in COVID-19 Model Mice
Authors: Alem Farhang , Campos-Obando Natalia , Narayanan Aarthi , Bailey Charles L. , Macaya Roman F.
Published: November 2023

A striking feature of COVID-19 disease is the broad spectrum of risk factors associated with case severity, as well as the diversity of clinical manifestations. While no central agent has been able to explain the pathogenesis of SARS-CoV-2 infection, the factors that most robustly correlate with severity are risk factors linked to aging. Low serum levels of Klotho, an anti-aging protein, strongly correlate with the pathogenesis of the same risk factors and manifestations of conditions similar to those expressed in severe COVID-19 cases. The current manuscript presents original research on the effects of the exogenous application of Klotho, an anti-aging protein, in COVID-19 model mice. Klotho supplementation resulted in a statistically significant survival benefit in parametric and non-parametric models. Further research is required to elucidate the mechanistic role Klotho plays in COVID-19 pathogenesis as well as the possible modulation SARS-CoV-2 may have on the biological aging process.
Dynamic and Seasonal Distribution of Enteric Viruses in Surface and Well Water in Riyadh (Saudi Arabia)
Authors: Abid Islem , Blanco Albert , Al-Otaibi Nawal , Guix Susana , Costafreda Maria I. , Pintó Rosa M. , Bosch Albert
Published: November 2023

Enteric viruses are the major cause of gastroenteritis and enteric hepatitis worldwide, but in some areas like Saudi Arabia, little is known about their presence in water sources. The available information from clinical samples is not enough to figure out their actual prevalence. The aim of this study was to gather information for the first time in Saudi Arabia on the presence of the Norovirus (NoV) genogroup GI and GII, hepatitis A virus (HAV), and hepatitis E virus (HEV) in water. For this purpose, thirteen monthly samples were collected from Lake Wadi Hanifa and surrounding wells from December 2014 to November 2015. Viruses were detected and quantified using real-time RT-qPCR. Despite HEV findings being anecdotic, our results highlight interesting behaviors of the other viruses. There was a higher prevalence of noroviruses in Wadi Hanifa samples than in well water samples (46.43% vs. 12.5% of NoV GI; 66.67% vs. 8.33% of NoV GII). On the contrary, similar levels of HAV positivity were observed (40.48% in surface water vs. 43.06% in well water). Also, a strong influence of flooding events on HAV and NoV GI occurrence was observed in both surface and well water samples, with NoV GII apparently not affected.
Permeation Protection by Waterproofing Mucosal Membranes
Authors: Coderch Luisa , Alonso Cristina , Calpena Ana Cristina , Pérez-García Maria Luisa , Clares-Naveros Beatriz , Ramos Anderson , Martí Meritxell
Published: November 2023

The permeability of the oral or nasal mucosa is higher than that of the skin. Mucosa permeability depends mainly on the thickness and keratinization degree of the tissues. Their permeability barrier is conditioned by the presence of certain lipids. This work has the main aim of reinforcing the barrier effect of oral mucosa with a series of formulations to reduce permeation. Transmembrane water loss of different formulations was evaluated, and three of them were selected to be tested on the sublingual mucosa permeation of drugs. Caffeine, ibuprofen, dexamethasone, and ivermectin were applied on porcine skin, mucosa, and modified mucosa in order to compare the effectiveness of the formulations. A similar permeation profile was obtained in the different membranes: caffeine > ibuprofen~dexamethasone > ivermectin. The most efficient formulation was a liposomal formulation composed of lipids that are present in the skin stratum corneum. Impermeability provided by this formulation was notable mainly for the low-molecular-weight compounds, decreasing their permeability coefficient by between 40 and 80%. The reinforcement of the barrier function of mucosa provides a reduction or prevention of the permeation of different actives, which could be extrapolated to toxic compounds such as viruses, contaminants, toxins, etc.