bluuebunny/crossref_metadata_2025 huggingface dataset:
Pretreatment Neutrophil-to-Lymphocyte Ratio as a Prognostic Biomarker in Unresectable or Metastatic Esophageal Cancer Patients With Anti-PD-1 Therapy
Authors: Gao Yiming , Zhang Zhibo , Li Yao , Chen Siyuan , Lu Jiangyue , Wu Liangliang , Ma Zhiqiang , Hu Yi , Zhang Guoqing
Published: April 2022

Background

The neutrophil-to-lymphocyte ratio (NLR) is an inflammatory index calculated by the absolute neutrophil count dividing the absolute lymphocyte count, and its prognostic role in esophageal cancer (EC) patients with anti-PD-1 therapy remains unclear.

Methods

A total of 140 unresectable or metastatic EC patients receiving PD-1 inhibitor treatment were included from Jan 2016 to Mar 2020. Kaplan–Meier method and log-rank test were used for comparing overall survival (OS) and progression-free survival (PFS) between groups. Multivariate Cox analysis was performed to assess the prognostic value of NLR.

Results

The cutoff value of NLR was set at 5, and the median follow-up time was 20.0 months. Patients with pretreatment NLR <5 had higher ORR (46.7% vs. 12.1%; p < 0.001) and DCR (85.0% vs. 69.7%; p = 0.047) than those with NLR ≥5. Kaplan–Meier curves showed that pretreatment NLR <5 was associated with longer PFS (median: 10.0 vs. 3.5 months, p < 0.0001) and OS (median: 22.3 vs. 4.9 months, p < 0.0001). Multivariate analysis demonstrated that pretreatment NLR ≥5 independently and significantly increased the risk of disease progression (hazard ratio (HR), 1.77 (95% confidence interval (CI), 1.12–2.82); p = 0.015) and death (HR, 4.01 (95% CI, 2.28–7.06); p < 0.001). Subgroup analysis showed that pretreatment NLR ≥5 was associated with poor efficacy and survival in most subsets.

Conclusions

Our findings showed that pretreatment NLR was independently and significantly associated with the efficacy and prognosis of EC patients treated with PD-1 inhibitors. NLR could serve as a convenient and useful prognostic biomarker for EC patients with anti-PD-1 therapy.
Esophageal Mucoepidermoid Carcinoma: A Review of 58 Cases
Authors: Wang Xin , Chen Yu-ping , Chen Shao-bin
Published: April 2022

Background

Esophageal mucoepidermoid carcinoma (EMEC) is a rare disease. The biological behavior and treatment of this malignancy are not well established.

Methods

Data from 58 patients with EMEC who underwent esophagectomy were retrospectively analyzed and compared with 5028 patients with esophageal squamous cell carcinoma (ESCC). Kaplan–Meier and multivariate Cox regression analyses were conducted to investigate the association between clinicopathological factors and survival.

Results

The study cohort included 36 males and 22 females with a median age of 59 years (range, 40-78 years). Of the 47 patients who underwent preoperative esophagoscopic biopsy, only 1 patient was diagnosed with EMEC. EMEC was more often found in female patients (39.7% versus 25.8%, P=0.036) and patients with EMEC had a significantly lower rate of lymph node metastasis (25.0% versus 49.4%, P<0.001) than patients with ESCC. After 1:1 propensity score matching, the 5-year overall survival rate of 55.2% for patients with EMEC was similar to that of 61.9% for patients with ESCC (P=0.399).

Conclusions

EMEC is a rare disease that more often affects females and these patients has less lymph node metastasis than patients with ESCC. Preoperative esophagoscopic biopsy has difficulty obtaining an accurate pathological diagnosis for EMEC patients. The prognosis for EMEC is similar to that for ESCC.
From Uterus to Brain: An Update on Epidemiology, Clinical Features, and Treatment of Brain Metastases From Gestational Trophoblastic Neoplasia
Authors: Borella Fulvio , Cosma Stefano , Ferraioli Domenico , Preti Mario , Gallio Niccolò , Valabrega Giorgio , Scotto Giulia , Rolfo Alessandro , Castellano Isabella , Cassoni Paola , Bertero Luca , Benedetto Chiara
Published: April 2022

In this review, we provide the state of the art about brain metastases (BMs) from gestational trophoblastic neoplasia (GTN), a rare condition. Data concerning the epidemiology, clinical presentation, innovations in therapeutic modalities, and outcomes of GTN BMs are comprehensively presented with particular attention to the role of radiotherapy, neurosurgery, and the most recent chemotherapy regimens. Good response rates have been achieved thanks to multi-agent chemotherapy, but brain involvement by GTNs entails significant risks for patients’ health since sudden and extensive intracranial hemorrhages are possible. Moreover, despite the evolution of treatment protocols, a small proportion of these patients ultimately develops a resistant disease. To tackle this unmet clinical need, immunotherapy has been recently proposed. The role of this novel option for this subset of patients as well as the achieved results so far are also discussed.
Successful Treatment of Carbamazepine-Induced Toxic Epidermal Necrolysis With Clinical Gastrointestinal Involvement: A Case Report
Authors: Chi Le Quynh , Anh Nguyen Thi Van , Le Nguyen Ngoc Quynh , Ha Nguyen Thi Thu , Tien Hoang Minh , Dien Tran Minh
Published: April 2022

Background

Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN) is a rare and life-threatening disease of the skin and mucosal surfaces. Although gastrointestinal manifestations in adults are potential prognostic factors for disease severity, there are limited data on such cases and their standard management in the pediatric population.

Case Presentation

We herein report the case of an 8-year-old girl with a 1-year history of epilepsy, who presented with bilateral conjunctivitis and progressively widespread bullous, and pruritic eruption based on erythematous skin after administration of carbamazepine. A diagnosis of carbamazepine-induced TEN was made, and the drug was immediately discontinued. The result of genetic screening showed that the patient was positive for the HLA-B*15:02 allele. Then, her condition got worse by developing gastrointestinal involvement, including hematemesis and severe watery bloody diarrhea. A combination of the intravenous immunoglobulin and the appropriate dose of systemic steroids have contributed to a favorable outcome in this case. Multidisciplinary care of mucocutaneous involvement, supplemental nutrition, and fluid replacement was also critically warranted. This report aims to contribute to the current literature on TEN-related gastrointestinal manifestations in pediatrics and highlights the need for further investigations in determining the optimal treatment in such cases.

Conclusion

In conclusion, we reported the successful treatment of TEN-related gastrointestinal manifestations in a pediatric patient, which should be critically considered in patients with SJS/TEN. Since it may significantly contribute to the poor prognosis of the illness, further investigations in determining standard management in such cases are necessary.
Adalimumab in Pediatric Inflammatory Bowel Disease
Authors: Choi So Yoon , Kang Ben
Published: April 2022

The introduction of biological agents with strong anti-inflammatory action, such as antitumor necrosis factor (TNF) agents, has changed inflammatory bowel disease (IBD) treatment strategy and goals, and has contributed significantly to improve the long-term prognosis of patients. Moreover, several biological agents are being used or researched in pediatric populations. However, only two biological agents, infliximab (IFX) and adalimumab (ADL), are currently approved for children and adolescents. In pediatric IBD, there are limitations and burdens associated with facilitating mucosal healing (MH) when utilizing these two biological agents. ADL is effective in both naïve patients and those with previous experience with biologics. Beyond clinical remission, this drug is also effective for MH and histological remission. The use of therapeutic drug monitoring to further enhance the effectiveness of ADL treatment can be expected to reduce treatment failure of ADL and pave the way for appropriate treatment in the treat-to-target era. This review paper focuses on ADL, examine studies conducted in children, and determine the role this agent plays against pediatric inflammatory bowel disease.
Limited Open Reduction and Transepiphyseal Intramedullary Kirschner Wire Fixation for Treatment of Irreducible Distal Radius Diaphyseal Metaphyseal Junction Fracture in Older Children
Authors: Wang Rufa , Wu Liwei , Wang Yinming , Fan Minjie , Wang Yiwei , Ning Bo , Zheng Pengfei
Published: April 2022

Objective

This study aimed to compare limited open reduction and transepiphyseal intramedullary fixation with Kirschner wire (LOR-TIKW) versus open reduction and internal fixation with plate and screw (ORIF-PS) for treatment of irreducible distal radius diaphyseal–metaphyseal junction (DMJ) fracture in older children.

Methods

Data of children (aged 10–14 years) treated in our hospital for distal radius DMJ fractures with LOR-TIKW or ORIF-PS from January 2018 to December 2019 were retrospectively analyzed. Follow-up was until radiographic union. Demographic, clinical, and radiographic data; treatment cost; healing time; functional outcome (by Price criteria); complications; and postoperative angulation and displacement were compared between children treated by the two methods. Statistical analysis was performed with alpha set at P < 0.05.

Results

A total of 26 children were included: 14 treated with LOR-TIKW and 12 with ORIF-PS. Operation time was less (22.1 min vs. 46.7 min, P < 0.0001), surgical incision smaller (2.43 cm vs. 5.00 cm, P < 0.0001), cost of internal fixation lower (US$, 40.6 vs. 2020, P < 0.0001), and healing time shorter (4.79 weeks vs. 5.64 weeks, P = 0.03) with LOR-TIKW; however, postoperative fracture angulation was slightly larger (1.07° vs. 0.83°, P = 0.85) and displacement slightly more (0.86 mm vs. 0.58 mm, P = 0.44) in the LOR-TIKW group. Rate of union, functional outcome, and complications were not significantly different between the groups.

Conclusion

For irreducible DMJ fracture of distal radius in older children, LOR-TIKW appears to be a promising method with several advantages over ORIF-PS.
Prediction of the Effects of Liraglutide on Kidney and Cardiovascular Outcomes Based on Short-Term Changes in Multiple Risk Markers
Authors: Tye Sok Cin , de Vries Sieta T. , Mann Johannes F. E. , Schechter Meir , Mosenzon Ofri , Denig Petra , Heerspink Hiddo J. L.
Published: April 2022

Aims: The LEADER trial demonstrated that the glucagon-like peptide-1 receptor agonist (GLP1-RA) liraglutide reduces kidney and cardiovascular (CV) risk in patients with type 2 diabetes. We previously developed a Parameter Response Efficacy (PRE) score that translates multiple short-term risk marker changes, from baseline to first available follow-up measurement, into a predicted long-term drug effect on clinical outcomes. The objective of this study was to assess the accuracy of the PRE score in predicting the efficacy of liraglutide in reducing the risk of kidney and CV outcomes.

Methods: Short-term changes in glycated hemoglobin (HbA1c), systolic blood pressure (BP), urinary-albumin-creatinine-ratio (UACR), hemoglobin, body weight, high-density-lipoprotein (HDL) cholesterol, low-density-lipoprotein (LDL) cholesterol, and potassium were monitored in the LEADER trial. Associations between risk markers and kidney or CV outcomes were established using a multivariable Cox proportional hazards model in a separate pooled database of 6,355 patients with type 2 diabetes. The regression coefficients were then applied to the short-term risk markers in the LEADER trial to predict the effects of liraglutide on kidney (defined as a composite of doubling of serum creatinine or end-stage kidney disease) and CV (defined as a composite of non-fatal myocardial infarction, non-fatal stroke, and CV death) outcomes.

Results: Liraglutide compared to placebo reduced HbA1c (1.4%), systolic BP (3.0 mmHg), UACR (13.2%), body weight (2.3 kg), hemoglobin (2.6 g/L), and increased HDL-cholesterol (0.01 mmol/L) (all p-values <0.01). Integrating multiple risk marker changes in the PRE score resulted in a predicted relative risk reduction (RRR) of 16.2% (95% CI 13.7–18.6) on kidney outcomes which was close to the observed RRR of 15.5% (95% CI -9.0–34.6). For the CV outcome, the PRE score predicted a 7.6% (95% CI 6.8–8.3) RRR, which was less than the observed 13.2% (95% CI 3.2–22.2) RRR.

Conclusion: Integrating multiple short-term risk markers using the PRE score adequately predicted the effect of liraglutide on the composite kidney outcome. However, the PRE score underestimated the effect of liraglutide for the composite CV outcome, suggesting that the risk markers included in the PRE score do not fully capture the CV benefit of liraglutide.
Application of an Accessible Interface for Pharmacokinetic Modeling and In Vitro to In Vivo Extrapolation
Authors: Hines David E. , Bell Shannon , Chang Xiaoqing , Mansouri Kamel , Allen David , Kleinstreuer Nicole
Published: April 2022

Regulatory toxicology testing has traditionally relied on in vivo methods to inform decision-making. However, scientific, practical, and ethical considerations have led to an increased interest in the use of in vitro and in silico methods to fill data gaps. While in vitro experiments have the advantage of rapid application across large chemical sets, interpretation of data coming from these non-animal methods can be challenging due to the mechanistic nature of many assays. In vitro to in vivo extrapolation (IVIVE) has emerged as a computational tool to help facilitate this task. Specifically, IVIVE uses physiologically based pharmacokinetic (PBPK) models to estimate tissue-level chemical concentrations based on various dosing parameters. This approach is used to estimate the administered dose needed to achieve in vitro bioactivity concentrations within the body. IVIVE results can be useful to inform on metrics such as margin of exposure or to prioritize potential chemicals of concern, but the PBPK models used in this approach have extensive data requirements. Thus, access to input parameters, as well as the technical requirements of applying and interpreting models, has limited the use of IVIVE as a routine part of in vitro testing. As interest in using non-animal methods for regulatory and research contexts continues to grow, our perspective is that access to computational support tools for PBPK modeling and IVIVE will be essential for facilitating broader application and acceptance of these techniques, as well as for encouraging the most scientifically sound interpretation of in vitro results. We highlight recent developments in two open-access computational support tools for PBPK modeling and IVIVE accessible via the Integrated Chemical Environment (https://ice.ntp.niehs.nih.gov/), demonstrate the types of insights these tools can provide, and discuss how these analyses may inform in vitro-based decision making.
Metformin and Thymoquinone Synergistically Inhibit Proliferation of Imatinib-Resistant Human Leukemic Cells
Authors: Glamoclija Una , Mahmutovic Lejla , Bilajac Esma , Soljic Violeta , Vukojevic Katarina , Suljagic Mirza
Published: April 2022

Chemotherapy resistance is one of the major challenges in cancer treatment, including leukemia. A massive array of research is evaluating combinations of drugs directed against different intracellular signaling molecules to overcome cancer resistance, increase therapy effectiveness, and decrease its adverse effects. Combining chemicals with proven safety profiles, such as drugs already used in therapy and active substances isolated from natural sources, could potentially have superior effects compared to monotherapies. In this study, we evaluated the effects of metformin and thymoquinone (TQ) as monotherapy and combinatorial treatments in chronic myeloid leukemia (CML) cell lines sensitive and resistant to imatinib therapy. The effects were also evaluated in primary monocytic acute myeloid leukemia (AML) and chronic lymphocytic leukemia (CLL) cells. Both compounds induced a dose- and time-dependent decrease of viability and proliferation in tested cells. Metformin had similar IC50 values in imatinib-sensitive and imatinib-resistant cell lines. IC50 values of TQ were significantly higher in imatinib-resistant cells, but with a limited resistance index (2.4). Synergistic effects of combinatorial treatments were observed in all tested cell lines, as well as in primary cells. The strongest synergistic effects were observed in the inhibition of imatinib-resistant cell line proliferation. Metformin and TQ inhibited the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling and induced apoptosis in tested cell lines and primary cells. The enhanced effects of combinatorial treatments on the induction of apoptosis were more dominant in imatinib-resistant compared to imatinib-sensitive CML cells. Primary cells were more sensitive to combinatorial treatments compared to cell lines. A combination of 1.25 mM metformin and 0.625 µM TQ increased the levels of cleaved poly (ADP-ribose) polymerase (PARP), decreased the levels of proliferation regulatory proteins, and inhibited protein kinase B (Akt) and NF-κB signaling in primary CLL cells. This study demonstrates that combinatorial treatments of imatinib-resistant malignant clones with metformin and TQ by complementary intracellular multi-targeting represents a promising approach in future studies.
Multiple Resonances Induced Terahertz Broadband Filtering in a Bilayer Metamaterial
Authors: Zeng Qiuming , Huang Yi , Zhong Shuncong , Lin Tingling , Zhong Yujie , Zhang Zhenghao , Yu Yingjie , Peng Zhike
Published: April 2022

A bilayer metamaterial to realize the broadband transmission in a terahertz (THz) filter was proposed, whose periodic unit structure consists of two rectangular apertures. The broadband consisting of three transmission peaks has a 3dB transmission range of 0.75 THz, which is 1.6 times that of the monolayer structure. Different from the traditional narrow band excitation mode, two additional transmission peaks are produced by the bilayer metamaterials. Sweeping frequency analysis has illustrated that the spacing between two layers of metamaterials has an influence on these additional transmission peaks. The bandwidth ranges can be regulated by adjusting the spacing at a proportional height. In particular, the experimental results show that the proposed filter has an excellent frequency selective performance with a bandwidth of 0.7 THz from 0.79 THz to 1.49 THz. This design of broadband filtering by introducing the bilayer metamaterial supplies a new approach with potential application in the THz broadband filter.
The Research of Long-Optical-Path Visible Laser Polarization Characteristics in Smoke Environment
Authors: Juntong Zhan , Shicheng Bao , Su Zhang , Qiang Fu , Yingchao Li , Jin Duan , Wei Zhang
Published: April 2022

The concentration of smoke in an environment can cause obvious interference to visible light intensity imaging, and it is a non-negligible factor in the polarized imaging of ground-based targets. Smoke particles cause severe scattering of photon intensity, resulting in polarization. In this case, low-visibility targets can be effectively identified by detecting the polarization dimension of targets. However, the polarization transmission of smoke in an environment is unclear, and the theoretical simulation lacks experimental reliability verification. To study this problem, this study constructs a polarization transmission model in a smoke environment and simulates and analyzes the scattering of visible polarized light at 450, 532, and 671 nm under different smoke densities. The optical thickness is determined to establish a reliable connection between the simulation and the external field long optical path test and verify the transmission of polarized light. Results show that the method has a 60% confidence in the polarization transmission model. With the increase in optical thickness, the degree of polarization (DOP) of the three wavelengths in the visible light band decreases, and the DOP of each polarized light decreases. No obvious difference is found between the DOPs of circularly polarized light at 450 nm and linearly polarized light. The DOP of circularly polarized light at 532 nm is 1–5% higher than that of linearly polarized light, 1–10% higher than that of the outdoor test, 1–5% higher than that of circularly polarized light at 671 nm, and 2–15% higher than that of the outdoor test. Therefore, the shorter the wavelength in the visible band, the higher the DOP. With the increase in wavelength, the polarization characteristics of circularly polarized light are gradually better than those of linearly polarized light.
The Renin-Angiotensin System as a Component of Biotrauma in Acute Respiratory Distress Syndrome
Authors: Krenn Katharina , Tretter Verena , Kraft Felix , Ullrich Roman
Published: April 2022

Acute respiratory distress syndrome (ARDS) is a major concern in critical care medicine with a high mortality of over 30%. Injury to the lungs is caused not only by underlying pathological conditions such as pneumonia, sepsis, or trauma, but also by ventilator-induced lung injury (VILI) resulting from high positive pressure levels and a high inspiratory oxygen fraction. Apart from mechanical factors that stress the lungs with a specific physical power and cause volutrauma and barotrauma, it is increasingly recognized that lung injury is further aggravated by biological mediators. The COVID-19 pandemic has led to increased interest in the role of the renin-angiotensin system (RAS) in the context of ARDS, as the RAS enzyme angiotensin-converting enzyme 2 serves as the primary cell entry receptor for severe acute respiratory syndrome (SARS) coronavirus (CoV)-2. Even before this pandemic, studies have documented the involvement of the RAS in VILI and its dysregulation in clinical ARDS. In recent years, analytical tools for RAS investigation have made major advances based on the optimized precision and detail of mass spectrometry. Given that many clinical trials with pharmacological interventions in ARDS were negative, RAS-modifying drugs may represent an interesting starting point for novel therapeutic approaches. Results from animal models have highlighted the potential of RAS-modifying drugs to prevent VILI or treat ARDS. While these drugs have beneficial pulmonary effects, the best targets and application forms for intervention still have to be determined to avoid negative effects on the circulation in clinical settings.
The Relationship Among Body Composition and Anaerobic Capacity and the Sport Level of Elite Male Motorcycle Speedway Riders
Authors: Michalik Kamil , Szczepan Stefan , Markowski Maciej , Zatoń Marek
Published: April 2022

The main objective of this study was to investigate the relationship among anaerobic capacity, body composition, and sport level of male junior and senior speedway riders. Sixty riders of professional clubs in the Polish top motorcycle speedway league participated in this study. They were divided into two equal groups (n = 30): junior (age = 19.7 ± 1.1 years) and senior (age = 29.7 ± 5.2). Body composition assessment, Wingate test (WAnT) on cycloergometer, with analysis of acute cardiorespiratory and biochemical responses were performed. Sport level was defined as the number of heats (races) won, winning percentage, total points scored during the season, and average points scored per heat. Seniors had higher point ratings indicating sport levels. As compared to the seniors, the juniors had lower BMI 4% (p < 0.01) and fat tissue mass by 20.5% (p < 0.01). A higher power decrease of 2.3% (p < 0.05) in the WAnT test was found in seniors. Body height negatively correlated with all indicators of seniors’ sport level (r = −0.41 to −0.55). Peak power output negatively correlated with seniors’ sport level (r = −0.39 to −0.41). Among the seniors, there was a negative correlation between post-exercise hydrogen ion concentration (r = −0.38), carbon dioxide partial pressure (r = −0.45) and average points scored per season. Conclusion: The anthropometric characteristics of body height, lean body mass and body surface area, are significantly correlated with the sport levels of the seniors motorcycle speedway riders. When selecting motorcycle speedway riders, use of these anthropometric characteristics may aid in determining the riders with the most potential to be successful. Metabolic acidosis tolerance and gas exchange efficiency show significance in seniors, indicating the need for intense exercise sessions.
Understanding Diversity, Evolution, and Structure of Small Heat Shock Proteins in Annelida Through in Silico Analyses
Authors: de la Fuente Mercedes , Novo Marta
Published: April 2022

Small heat shock proteins (sHsps) are oligomeric stress proteins characterized by an α-crystallin domain (ACD). These proteins are localized in different subcellular compartments and play critical roles in the stress physiology of tissues, organs, and whole multicellular eukaryotes. They are ubiquitous proteins found in all living organisms, from bacteria to mammals, but they have never been studied in annelids. Here, a data set of 23 species spanning the annelid tree of life, including mostly transcriptomes but also two genomes, was interrogated and 228 novel putative sHsps were identified and manually curated. The analysis revealed very high protein diversity and showed that a significant number of sHsps have a particular dimeric architecture consisting of two tandemly repeated ACDs. The phylogenetic analysis distinguished three main clusters, two of them containing both monomeric sHsps, and ACDs located downstream in the dimeric sHsps, and the other one comprising the upstream ACDs from those dimeric forms. Our results support an evolutionary history of these proteins based on duplication events prior to the Spiralia split. Monomeric sHsps 76) were further divided into five subclusters. Physicochemical properties, subcellular location predictions, and sequence conservation analyses provided insights into the differentiating elements of these putative functional groups. Strikingly, three of those subclusters included sHsps with features typical of metazoans, while the other two presented characteristics resembling non-metazoan proteins. This study provides a solid background for further research on the diversity, evolution, and function in the family of the sHsps. The characterized annelid sHsps are disclosed as essential for improving our understanding of this important family of proteins and their pleotropic functions. The features and the great diversity of annelid sHsps position them as potential powerful molecular biomarkers of environmental stress for acting as prognostic tool in a diverse range of environments.
A Multi-Omics Approach for Rapid Identification of Large Genomic Lesions at the Wheat Dense Spike (wds) Locus
Authors: Wang Zhenyu , Tao Shu , Liu Shaoshuai , Jia Meiling , Cui Dada , Sun Guoliang , Deng Zhongyin , Wang Fang , Kong Xingchen , Fu Mingxue , Che Yuqing , Liao Ruyi , Li Tao , Geng Shuaifeng , Mao Long , Li Aili
Published: April 2022

Optimal spike architecture provides a favorable structure for grain development and yield improvement. However, the number of genes cloned to underlie wheat spike architecture is extremely limited. Here, we obtained a wheat dense spike mutant (wds) induced by 60Co treatment of a common wheat landrace Huangfangzhu that exhibited significantly reduced spike and grain lengths. The shortened spike length was caused by longitudinal reduction in number and length of rachis cells. We adopted a multi-omics approach to identify the genomic locus underlying the wds mutant. We performed Exome Capture Sequencing (ECS) and identified two large deletion segments, named 6BL.1 at 334.8∼424.3 Mb and 6BL.2, 579.4∼717.8 Mb in the wds mutant. RNA-seq analysis confirmed that genes located in these regions lost their RNA expression. We then found that the 6BL.2 locus was overlapping with a known spike length QTL, qSL6B.2. Totally, 499 genes were located within the deleted region and two of them were found to be positively correlated with long spike accessions but not the ones with short spike. One of them, TraesCS6B01G334600, a well-matched homolog of the rice OsBUL1 gene that works in the Brassinosteroids (BR) pathway, was identified to be involved in cell size and number regulation. Further transcriptome analysis of young spikes showed that hormone-related genes were enriched among differentially expressed genes, supporting TraesCS6B01G334600 as a candidate gene. Our work provides a strategy to rapid locate genetic loci with large genomic lesions in wheat and useful resources for future wheat study.
5S Ribosomal DNA of Genus Solanum: Molecular Organization, Evolution, and Taxonomy
Authors: Tynkevich Yurij O. , Shelyfist Antonina Y. , Kozub Liudmyla V. , Hemleben Vera , Panchuk Irina I. , Volkov Roman A.
Published: April 2022

TheSolanumgenus, being one of the largest among high plants, is distributed worldwide and comprises about 1,200 species. The genus includes numerous agronomically important species such asSolanum tuberosum(potato),Solanum lycopersicum(tomato), andSolanum melongena(eggplant) as well as medical and ornamental plants. The hugeSolanumgenus is a convenient model for research in the field of molecular evolution and structural and functional genomics. Clear knowledge of evolutionary relationships in theSolanumgenus is required to increase the effectiveness of breeding programs, but the phylogeny of the genus is still not fully understood. The rapidly evolving intergenic spacer region (IGS) of 5S rDNA has been successfully used for inferring interspecific relationships in several groups of angiosperms. Here, combining cloning and sequencing with bioinformatic analysis of genomic data available in the SRA database, we evaluate the molecular organization and diversity of IGS for 184 accessions, representing 137 species of theSolanumgenus. It was found that the main mechanisms of IGS molecular evolution was step-wise accumulation of single base substitution or short indels, and that long indels and multiple base substitutions, which arose repeatedly during evolution, were mostly not conserved and eliminated. The reason for this negative selection seems to be association between indels/multiple base substitutions and pseudogenization of 5S rDNA. Comparison of IGS sequences allowed us to reconstruct the phylogeny of theSolanumgenus. The obtained dendrograms are mainly congruent with published data: same major and minor clades were found. However, relationships between these clades and position of some species (S. cochoae, S. clivorum, S. macrocarpon, andS. spirale) were different from those of previous results and require further clarification. Our results show that 5S IGS represents a convenient molecular marker for phylogenetic studies on theSolanumgenus. In particular, the simultaneous presence of several structural variants of rDNA in the genome enables the detection of reticular evolution, especially in the largest and economically most important sect.Petota. The origin of several polyploid species should be reconsidered.
Inter-Individual Budburst Variation in Fagus sylvatica Is Driven by Warming Rate
Authors: Malyshev Andrey V. , van der Maaten Ernst , Garthen Aron , Maß Dennis , Schwabe Matthias , Kreyling Juergen
Published: April 2022

The onset of the growing season in temperate forests is relevant for forest ecology and biogeochemistry and is known to occur earlier with climate change. Variation in tree phenology among individual trees of the same stand and species, however, is not well understood. Yet, natural selection acts on this inter-individual variation, which consequently affects the adaptive potential to ongoing environmental changes. Budburst dates of 146 mature individuals of Fagus sylvatica, the dominant natural forest tree of central Europe, were recorded over 12 years in one forest stand of 1 ha in the Müritz National Park, Germany. The tree-specific location, topographical differences, as well as social status, were measured to explain the inter-individual variation in budburst. Furthermore, inter-individual differences in bud dormancy were quantified. Additional phenology and weather data across Germany from 405 sites over a 25-year period was used to put the insights from the single stand into perspective. Consistent phenological ranking over the years with respect to early and late flushing trees was observed within the single forest stand, with 23 trees consistently flushing 3–6 days earlier and 22 trees consistently flushing 3–10 days later than the median. Trees flushing consistently early varied most in their spring budburst dates and were less dormant than late-flushing trees already in mid-winter. The higher variation in earlier flushing trees was best explained by a slower warming rate during their budburst period in the observed stand as well as across Germany. Likewise, years with a lower warming rate during the budburst period were more variable in budburst dates. The rate of warming during spring time is crucial to accurately project future within-species variation and the resulting adaptive potential in spring phenology of dominant forest tree species.
Quantitative Extraction and Evaluation of Tomato Fruit Phenotypes Based on Image Recognition
Authors: Zhu Yihang , Gu Qing , Zhao Yiying , Wan Hongjian , Wang Rongqing , Zhang Xiaobin , Cheng Yuan
Published: April 2022

Tomato fruit phenotypes are important agronomic traits in tomato breeding as a reference index. The traditional measurement methods based on manual observation, however, limit the high-throughput data collection of tomato fruit morphologies. In this study, fruits of 10 different tomato cultivars with considerable differences in fruit color, size, and other morphological characters were selected as samples. Constant illumination condition was applied to take images of the selected tomato fruit samples. Based on image recognition, automated methods for measuring color and size indicators of tomato fruit phenotypes were proposed. A deep learning model based on Mask Region-Convolutional Neural Network (R-CNN) was trained and tested to analyze the internal structure indicators of tomato fruit. The results revealed that the combined use of these methods can extract various important fruit phenotypes of tomato, including fruit color, horizontal and vertical diameters, top and navel angles, locule number, and pericarp thickness, automatically. Considering several corrections of missing and wrong segmentation cases in practice, the average precision of the deep learning model is more than 0.95 in practice. This suggests a promising locule segmentation and counting performance. Vertical/horizontal ratio (fruit shape index) and locule area proportion were also calculated based on the data collected here. The measurement precision was comparable to manual operation, and the measurement efficiency was highly improved. The results of this study will provide a new option for more accurate and efficient tomato fruit phenotyping, which can effectively avoid artificial error and increase the support efficiency of relevant data in the future breeding work of tomato and other fruit crops.
Digitalization in Candidate Selection. Support and Resistance Within Established Political Parties in Germany
Authors: Bloquet Claire , Borucki Isabelle , Höhne Benjamin
Published: April 2022

The digitalization of human life has impacted many aspects of politics in the last two decades. Intra-party decision-making is one of them. While new political parties appear to be rather native digital organizations, established parties are increasingly beginning to incorporate online tools into their internal processes. However, not much is known about how intra-party selectorates evaluate the digitalization of a crucial decision-making process. This study asks whether party members who participate in candidate selection support online consultations—or not. Using an original large-N dataset on the preferences of party members attending candidate selection assemblies for the German Bundestag, we determine variables that increase or decrease the likelihood to support the introduction of online consultations as part of intra-party democracy. Our results show that attitudes toward digitalization do not depend on a generational or a partisan factor, as might have been expected. Instead, we highlight that digitalization support is first and foremost related to, on the one hand, the seniority in the party, and, on the other, on one's preferences toward inclusion. We relate these findings to the distribution of powers and incentives within the party and discuss both the implications of these results and what they might mean for established parties trying to reform.
Gambling, Gaming, and Internet Behavior in a Sexual Minority Perspective. A Cross-Sectional Study in Seven European Countries
Authors: Broman Niroshani , Prever Fulvia , di Giacomo Ester , Jiménez-Murcia Susana , Szczegielniak Anna , Hansson Helena , Håkansson Anders
Published: April 2022

Background

Addictive behavior of gambling, gaming and internet activity is partly a new research domain and has not been well investigated with regard to sexual minority populations. Although health disparities between sexual minorities and the general population are well documented, there is a lack of inclusion of sexual minorities in both research and clinic. Among lesbian, gay and bisexual populations certain features could be present that play a role for the development of addictive behaviors, such as social isolation and increased risk of other psychiatric problems. The aim of this study was to investigate problem gambling, problem gaming and problematic internet behavior in a European context and if it is affected by sexual orientation status.

Methods

An online web-survey was distributed among web-panels in England, Poland, Switzerland, Italy, Spain, Denmark, and Sweden in 2017–2018.

Result

10 983 complete answers were collected. 7.1% of the participants had a sexual minority status (n = 774). Regression models found that there was no difference in gambling, gaming and internet behavior among heterosexual and sexual minority men. Sexual minority women were associated with problematic gambling and gaming behavior, when also controlling for age and nationality. When also controlling for psychological distress, women defining as having another sexual minority status than lesbian and bisexual remained significant for having a problematic gaming behavior (AOR = 2.3).

Conclusion

An awareness of female sexual minority perspectives is relevant in facilities treating behavioral addiction as well as in future research in behavioral addiction. More research is needed in problematic gambling and gaming behavior in different sexual minority populations with regard to psychiatric comorbidity and living conditions. An inclusion of sexual minority groups defining as other than gay and bisexual is needed in future research. No significant differences were found between heterosexual and sexual minority men in adjusted analysis in this study.
Social Achievement Goals in Chinese Undergraduates: Associations With Self-Esteem and Symptoms of Social Anxiety and Depression
Authors: Zhao Yanhua
Published: April 2022

The pursuit of relationship goals is critical to the wellbeing of young adults. This study investigated different achievement goals toward social competence as potential predictors of social anxiety and depression symptoms. It proposed that self-esteem may function as a mediator on the pathway from endorsing social achievement goals to undergraduates' concurrent and longitudinal social anxiety and depression symptoms. Social achievement goal theory proposes three types of goals: social mastery goals (striving to improve one's social competence), social performance-approach goals (striving to prove one's social competence and win positive evaluation), and social performance-avoid goals (striving to avoid incompetent social behaviors and negative evaluation). One hundred and eighty-five Chinese undergraduates aged from 18 to 23 (50% female) completed this study across two-time points. Path analyses indicated that social mastery (marginally) and performance-approach goals were positively associated with self-esteem, whereas social performance-avoid goals were negatively associated with self-esteem; self-esteem was negatively associated with the concurrent social anxiety and depression symptoms and the longitudinal depression symptoms. The proposed mediation effects of self-esteem on the links from three types of social achievement goals to the concurrent and longitudinal social anxiety and depression symptoms were significant except on the links from social mastery goals and social performance-approach goals to the subsequent social anxiety symptoms. Self-esteem and the baseline social anxiety and depressive symptoms have a chain mediating effect between social achievement goals and the longitudinal symptoms of social anxiety and depression. These findings suggest that the pursuit of social mastery goals and performance-approach goals in initiating and maintaining social relationships boosts undergraduates' self-worth and reduces their concurrent and longitudinal depression experiences. However, the strivings to hide inadequacy and avoid negative evaluation in social contexts impede one's self-worth and increase concurrent and longitudinal social anxiety and depression symptoms. Implications and limitations are discussed.
Job Burnout Is Associated With Prehospital Decision Delay: An Internet-Based Survey in China
Authors: Yin Han , Jiang Cheng , Shi Xiaohe , Chen Yilin , Yu Xueju , Wang Yu , Li Weiya , Ma Huan , Geng Qingshan
Published: April 2022

Background

Prehospital delay is associated with non-modifiable factors such as age, residential region, and disease severity. However, the impact of psychosocial factors especially for job burnout on prehospital decision delay is still little understood.

Method

This internet-based survey was conducted between 14 February 2021 and 5 March 2021 in China through the Wechat platform and web page. Self-designed questionnaires about the expected and actual length of prehospital decision time and the Chinese version of Maslach Burnout Inventory-General Survey, Type D Personality Scale-14, and Social Support Rating Scale were applied. A total of 1,039 general participants with a history of perceptible but tolerable body discomfort were included.

Results

The top six reasons for prehospital decision delay were: (1) endure until self-healing (50.7%), (2) too busy to ask for leave (40.3%), (3) process for seeing a doctor too complicated (35.8%), (4) too tired after work (26.2%), (5) worry about the expenditure (16.6%), and (6) fear of being identified as with serious problem (14.5%). The univariate analyses revealed that older age (p = 0.001), type D personality (p = 0.025), job burnout (p = 0.055), and worrying about expenditure (p = 0.004) were associated with prolonged prehospital decision time, while engaged in medical-related job (p = 0.028) and with more social support (p = 0.066) would shorten the delay. The multivariate analysis using logistic regression model with forward selecting method showed that age [per 10 years, odds ratio (OR) 1.19 (1.09–1.31), p < 0.001], job burnout [per 10 points in Maslach Burnout Inventory-General Survey (MBI-GS), OR 1.17 (1.04–1.31), p = 0.007], and worrying about expenditure [OR 1.75 (1.25–2.47), p = 0.001] were the three determinants for prehospital decision delay (>7 days). Mediating effects were analyzed by using bias-corrected percentile bootstrap methods (N = 10,000). Social support was found partially mediated the relationship between the determinants and prehospital decision time. The partial mediating effect of social support accounted for 24.0% of the total effect for job burnout and 11.6% for worrying about expenditure.

Conclusion

Psychosocial factors have a non-negligible impact on prehospital decision delay. The crucial part of prehospital decision delay may be the lack of motivation inside. Job burnout and lack of social support, as two commonly seen features in the modern world, should be given enough consideration in disease prevention and treatment.
No Significant Changes in Addictive and Problematic Behaviors During the COVID-19 Pandemic and Related Lockdowns: A Three-Wave Longitudinal Study
Authors: Koós Mónika , Demetrovics Zsolt , Griffiths Mark D. , Bőthe Beáta
Published: April 2022

Introduction

The COVID-19 outbreak and related lockdowns brought substantial changes in people’s lives and led to concerns about possible increases of addictive behaviors at the initial stages of the pandemic. To examine these concerns, the aim of the present study was to assess longitudinal changes in addictive and problematic behaviors (i.e., problematic social media use, Internet gaming disorder, gambling disorder, problematic pornography use, and compulsive sexual behavior disorder) over time during the COVID-19 pandemic.

Methods

Three waves of data collection took place in different stages of the COVID-19 outbreak in Hungary in a general population, from the first wave of lockdowns to the second and third waves of restrictions (May, 2020; NT1 = 1747; June–August, 2020; NT2 = 656; January, 2021; NT3 = 411). Latent growth curve models were calculated to assess the potential changes in addictive and problematic behaviors over time.

Results

Latent growth curve models showed that the sample varied in their initial scores, but there were no significant changes over time in any of the examined behaviors, except for compulsive sexual behavior disorder, which demonstrated a small but significant increase (i.e., positive and significant slope factor). However, the rate of this change was negligible. Overall, there were no noteworthy changes over time regarding any of the examined addictive and problematic behaviors.

Conclusion

Contrary to initial concerns, no substantial changes over time were observed regarding the examined addictive behaviors during the COVID-19 pandemic and related lockdowns. These findings indicate that those who had no previous problem with these addictive behaviors, might have not developed a problem, and those who had problem with either of the behaviors previously, might have not experienced a significant increase in their symptoms.
The Global 2000-2020 Land Cover and Land Use Change Dataset Derived From the Landsat Archive: First Results
Authors: Potapov Peter , Hansen Matthew C. , Pickens Amy , Hernandez-Serna Andres , Tyukavina Alexandra , Turubanova Svetlana , Zalles Viviana , Li Xinyuan , Khan Ahmad , Stolle Fred , Harris Nancy , Song Xiao-Peng , Baggett Antoine , Kommareddy Indrani , Kommareddy Anil
Published: April 2022

Recent advances in Landsat archive data processing and characterization enhanced our capacity to map land cover and land use globally with higher precision, temporal frequency, and thematic detail. Here, we present the first results from a project aimed at annual multidecadal land monitoring providing critical information for tracking global progress towards sustainable development. The global 30-m spatial resolution dataset quantifies changes in forest extent and height, cropland, built-up lands, surface water, and perennial snow and ice extent from the year 2000 to 2020. Landsat Analysis Ready Data served as an input for land cover and use mapping. Each thematic product was independently derived using locally and regionally calibrated machine learning tools. Thematic maps validation using a statistical sample of reference data confirmed their high accuracy (user’s and producer’s accuracies above 85% for all land cover and land use themes, except for built-up lands). Our results revealed dramatic changes in global land cover and land use over the past 20 years. The bitemporal dataset is publicly available and serves as a first input for the global land monitoring system.