bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel Nanoarchitectured Cu2Te as a Photocathodes for Photoelectrochemical Water Splitting Applications
Authors: Lee Dong Jin , Mohan Kumar G. , Ganesh V. , Jeon Hee Chang , Kim Deuk Young , Kang Tae Won , Ilanchezhiyan P.
Published: September 2022

Designing photocathodes with nanostructures has been considered a promising way to improve the photoelectrochemical (PEC) water splitting activity. Cu2Te is one of the promising semiconducting materials for photoelectrochemical water splitting, the performance of Cu2Te photocathodes remains poor. In this work, we report the preparation of Cu2Te nanorods (NRs) and vertical nanosheets (NSs) assembled film on Cu foil through a vapor phase epitaxy (VPE) technique. The obtained nano architectures as photocathodes toward photoelectrochemical (PEC) performance was tested afterwards for the first time. Optimized Cu2Te NRs and NSs photocathodes showed significant photocurrent density up to 0.53 mA cm−2 and excellent stability under illumination. Electrochemical impedance spectroscopy and Mott–Schottky analysis were used to analyze in more detail the performance of Cu2Te NRs and NSs photocathodes. From these analyses, we propose that Cu2Te NRs and NSs photocathodes are potential candidate materials for use in solar water splitting.
Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin
Authors: Sredojević Dušan , Lazić Vesna , Pirković Andrea , Periša Jovana , Murafa Natalija , Spremo-Potparević Biljana , Živković Lada , Topalović Dijana , Zarubica Aleksandra , Jovanović Krivokuća Milica , Nedeljković Jovan M.
Published: September 2022

The antibacterial performance and cytotoxic examination of in situ prepared silver nanoparticles (Ag NPs), on inorganic-organic hybrid nanopowder consisting of zirconium dioxide nanoparticles (ZrO2 NPs) and dihydroquercetin (DHQ), was performed against Gram (−) bacteria Escherichia coli and Gram (+) bacteria Staphylococcus aureus, as well as against human cervical cancer cells HeLa and healthy MRC-5 human cells. The surface modification of ZrO2 NPs, synthesized by the sol-gel method, with DHQ leads to the interfacial charge transfer (ICT) complex formation indicated by the appearance of absorption in the visible spectral range. The prepared samples were thoroughly characterized (TEM, XRD, reflection spectroscopy), and, in addition, the spectroscopic observations are supported by the density functional theory (DFT) calculations using a cluster model. The concentration- and time-dependent antibacterial tests indicated a complete reduction of bacterial species, E. coli and S. aureus, for all investigated concentrations of silver (0.10, 0.25, and 0.50 mg/mL) after 24 h of contact. On the other side, the functionalized ZrO2 NPs with DHQ, before and after deposition of Ag NPs, do not display a significant decrease in the viability of HeLa MRC-5 cells in any of the used concentrations compared to the control.
Synthesis of Highly Monodisperse Nickel and Nickel Phosphide Nanoparticles
Authors: Cho Hyungjin , Lee Nohyun , Kim Byung Hyo
Published: September 2022

Nickel and nickel phosphide nanoparticles are highly useful in various fields, owing to their catalytic and magnetic properties. Although several synthetic protocols to produce nickel and nickel phosphide nanoparticles have been previously proposed, controllable synthesis of nanoparticles using these methods is challenging. Herein, we synthesized highly monodisperse nickel and nickel phosphide nanoparticles via thermal decomposition of nickel–oleylamine–phosphine complexes in organic solvents. The size and composition of the nickel and nickel phosphide nanoparticles were easily controlled by changing the aging temperature, precursor concentration, and phosphine surfactant type. Large-sized monodisperse nickel nanoparticles obtained using our method were successfully applied for the purification of histidine-tagged proteins.
Vitamin D3 Supplementation in Overweight/Obese Pregnant Women: No Effects on the Maternal or Fetal Lipid Profile and Body Fat Distribution—A Secondary Analysis of the Multicentric, Randomized, Controlled Vitamin D and Lifestyle for Gestational Diabetes Prevention Trial (DALI)
Authors: Harreiter Jürgen , Mendoza Lilian C. , Simmons David , Desoye Gernot , Devlieger Roland , Galjaard Sander , Damm Peter , Mathiesen Elisabeth R. , Jensen Dorte M. , Andersen Lise Lotte T. , Dunne Fidelma , Lapolla Annunziata , Dalfra Maria G. , Bertolotto Alessandra , Wender-Ozegowska Ewa , Zawiejska Agnieszka , Hill David , Jelsma Judith G. M. , Snoek Frank J. , Worda Christof , Bancher-Todesca Dagmar , van Poppel Mireille N. M. , Corcoy Rosa , Kautzky-Willer Alexandra ,   on behalf of the DALI Core Investigator Group
Published: September 2022

Vitamin D deficiency is a common finding in overweight/obese pregnant women and is associated with increased risk for adverse pregnancy outcome. Both maternal vitamin D deficiency and maternal obesity contribute to metabolic derangements in pregnancy. We aimed to assess the effects of vitamin D3 supplementation in pregnancy versus placebo on maternal and fetal lipids. Main inclusion criteria were: women <20 weeks’ gestation, BMI ≥ 29 kg/m2. Eligible women (n = 154) were randomized to receive vitamin D3 (1600 IU/day) or placebo. Assessments were performed <20, 24–28 and 35–37 weeks and at birth. Linear regression models were used to assess effects of vitamin D on maternal and cord blood lipids. In the vitamin D group significantly higher total 25-OHD and 25-OHD3 levels were found in maternal and cord blood compared with placebo. Adjusted regression models did not reveal any differences in triglycerides, LDL-C, HDL-C, free fatty acids, ketone bodies or leptin between groups. Neonatal sum of skinfolds was comparable between the two groups, but correlated positively with cord blood 25-OH-D3 (r = 0.34, p = 0.012). Vitamin D supplementation in pregnancy increases maternal and cord blood vitamin D significantly resulting in high rates of vitamin D sufficiency. Maternal and cord blood lipid parameters were unaffected by Vitamin D3 supplementation.
A Prognostic Model Based on Nutritional Risk Index in Operative Breast Cancer
Authors: Lin Fei , Xia Wen , Chen Miao , Jiang Tongchao , Guo Jia , Ouyang Yi , Sun Haohui , Chen Xiaoyu , Deng Wuguo , Guo Ling , Lin Huanxin
Published: September 2022

Background: The nutritional risk index (NRI) is an independent prognostic factor for overall survival in various cancers, but its prognostic value in breast cancer remains unclear. This study aimed to explore the relationship between the NRI and overall survival (OS) in breast cancer and to develop a predictive nomogram. Methods: We retrospectively enrolled 1347 breast cancer patients who underwent mastectomy or lumpectomy between January 2011 and November 2012. Using a cutoff value of 110.59, patients were divided into a high-NRI group and a low-NRI group. OS was compared between the two groups. Clinicopathological factors independently associated with survival were used to construct a predictive nomogram. Results: Of the 1347 patients, 534 patients were classified as high NRI and 813 as low NRI. OS was significantly shorter in low-NRI patients. The 3- and 5-year OS rates were 87.3% and 73.4%, respectively, in the high-NRI group whereas they were 83.0% and 67.2%, respectively, in the low-NRI group. Cox regression analysis found that histopathological type, tumor size, lymph node status, progesterone receptor (PR) status, Ki-67, and NRI were independently associated with OS. Conclusions: NRI is an independent prognostic factor of OS in breast cancer patients. The proposed nomogram model may be a useful tool for individualized survival prediction.
Healthy Lifestyle Is Associated with Reduced Mortality in Patients with Non-Alcoholic Fatty Liver Disease
Authors: Yu Chengxiao , Gao Jiaxin , Ge Xinyuan , Wang Xiao , Ding Yuqing , Tian Ting , Xu Xin , Guo Wen , Wang Quanrongzi , Ge Zijun , Jiang Tao , Zhang Qun , Song Ci
Published: September 2022

Background and Aims: It is unclear whether a healthy lifestyle impacts mortality in the presence of non-alcoholic fatty liver disease (NAFLD). The present study aimed to examine the joint association of several modifiable lifestyle factors with mortality risk for NAFLD patients. Methods: We collected lifestyle behavior data form the National Health and Nutrition Examination Survey (NHANES) III from 1988 to 1994 and follow-up data form NHANES III-linked mortality data through 2015. We estimated joint association between four healthy lifestyle factors (non-smoking, non-drinking, regular physical activity, a healthy diet) after NAFLD diagnosis and mortality using Cox proportional hazards regression models. Results: During a median of 22.83 years of follow-up, 2932 deaths occurred. The risk of all-cause mortality decreased significantly with the healthy lifestyle scores increasing (p < 0.001). NAFLD patients with a favorable lifestyle (3 or 4 healthy lifestyle factors) reduced 36% of all-cause mortality and 43% of cardiovascular disease (CVD) mortality compared with those with an unfavorable lifestyle (0 or 1 healthy lifestyle factor) (HR, 0.64 [95% CI, 0.50–0.81], 0.57 [95% CI, 0.37–0.88]). Compared with the non-NAFLD group, the number of NAFLD patients required to adhere to a favorable lifestyle to prevent one cardiovascular disease death in 20 years was fewer (77 vs. 125). Conclusions: For the NAFLD patients, adopting a healthy lifestyle could significantly reduce their risk of death.
Updated Meal Patterns in the Child and Adult Care Food Program and Changes in Quality of Food and Beverages Served: A Natural Experimental Study
Authors: Andreyeva Tatiana , Mozaffarian Rebecca S. , Kenney Erica L.
Published: September 2022

With diet-related chronic diseases being the largest contributors to U.S. morbidity and mortality, identifying population-level strategies to promote healthier diets is essential. Intervention during early childhood may be particularly important. The Child and Adult Care Food Program (CACFP), a federal nutrition assistance program in the U.S. that supports serving meals and snacks in child care settings, reaches millions of U.S. children. Recent 2017 updates to CACFP’s meal patterns were meant to improve the nutritional quality of food served through CACFP by providing more whole grains, fruit, and vegetables. In this study, we used a natural experimental, longitudinal study of child care centers participating in CACFP compared to nonparticipating centers to assess whether the quality of food and beverages served (per menu analysis) improved following the CACFP meal pattern changes. While we found that CACFP centers were more likely to meet several key nutrition standards in comparison to non-CACFP centers overall, there were no differences in menu quality from before to after the 2017 standards change between CACFP and non-CACFP centers. Nutrition standards for CACFP may need to be further strengthened with adequate financial and technical support given to child care programs for effective implementation.
Wentilactone A Reverses the NF-κB/ECM1 Signaling-Induced Cisplatin Resistance through Inhibition of IKK/IκB in Ovarian Cancer Cells
Authors: Lv Cuiting , Ren Chunxia , Yu Yinjue , Yin Huijing , Huang Caiguo , Yang Gong , Hong Yang
Published: September 2022

Wentilactone A (WA) is a tetranorditerpenoid isolated from marine algae. We previously found that WA inhibited cancer cell proliferation with little toxicity. In this study, we show that high expression of extracellular matrix protein-1 (ECM1) promotes cancer cell cisplatin resistance, and the secreted ECM1 activates normal fibroblasts (NFs) to transform cells with characteristics of cancer-associated fibroblasts (CAFs). Transcription of the ECM1 gene is regulated largely by NF-κB through EP881C/T-EP266C binding sites. WA supresses the phosphorylation of NF-κB through inhibition of the upstream IKK/IκB phoshorylation to block the expression of ECM1, which reverses the cisplatin-induced activation of NF-κB/ECM1. On the contrary, cisplatin facilitates phosphorylation of NF-κB to enhance the expression of ECM1. These results highlight ECM1 as a potential target for treatment of cisplatin-resistant cancers associated with the ECM1 activated signaling. In addition, WA reverses cisplatin resistance by targeting both tumor cells and the tumor microenvironment through IKK/IκB/NF-κB signaling to reduce the expression of the ECM1 protein.
Combination Treatment with Hydroxytyrosol and Vitamin E Improves NAFLD-Related Fibrosis
Authors: Panera Nadia , Braghini Maria Rita , Crudele Annalisa , Smeriglio Antonella , Bianchi Marzia , Condorelli Angelo Giuseppe , Nobili Rebecca , Conti Libenzio Adrian , De Stefanis Cristiano , Lioci Gessica , Gurrado Fabio , Comparcola Donatella , Mosca Antonella , Sartorelli Maria Rita , Scoppola Vittorio , Svegliati-Baroni Gianluca , Trombetta Domenico , Alisi Anna
Published: September 2022

Non-alcoholic fatty liver disease (NAFLD)-related liver fibrosis results in the encapsulation of injured liver parenchyma by a collagenous scar mainly imputable to hepatic stellate cells’ activation. Approved pharmacological treatments against NAFLD-related fibrosis are still lacking, but natural compounds such as hydroxytyrosol (HXT) and vitamin E (VitE), are emerging as promising therapeutic opportunities. In this study, the potential anti-fibrotic effect of HXT + VitE combination therapy was investigated in vitro and in vivo. In particular, tumor growth factor (TGF)-β-activated LX-2 cells as an in vitro model, and carbon tetrachloride plus a Western diet as a mice model were employed. The effect of HXT + VitE on fibrosis was also investigated in children with biopsy-proven NAFLD. Our results demonstrated that HXT + VitE caused a reduction of proliferation, migration, contractility, and expression of pro-fibrogenic genes in TGF-β-activated LX-2 cells. HXT + VitE treatment also antagonized TGF-β-dependent upregulation of pro-oxidant NOX2 by interfering with nuclear translocation/activation of SMAD2/3 transcription factors. The mouse model of NAFLD-related fibrosis treated with HXT + VitE showed a marked reduction of fibrosis pattern by histology and gene expression. Accordingly, in children with NAFLD, HXT + VitE treatment caused a decrease of circulating levels of PIIINP and NOX2 that was supported over time. Our study suggests that HXT + VitE supplementation may improve NAFLD-related fibrosis.
Barriers and Facilitators of Online Grocery Services: Perceptions from Rural and Urban Grocery Store Managers
Authors: Gillespie Rachel , DeWitt Emily , Trude Angela C. B. , Haynes-Maslow Lindsey , Hudson Travis , Anderson-Steeves Elizabeth , Barr Makenzie , Gustafson Alison
Published: September 2022

Online grocery shopping has expanded rapidly in the U.S., yet little is known about the retailer’s perceptions of online grocery services, which can aid in the expansion of services. Furthermore, many barriers to online grocery utilization persist across geographic areas, especially among Supplemental Nutrition Assistance Program (SNAP)-authorized retailers. This study captured perceived barriers and facilitators of online grocery shopping for managers of SNAP-authorized retailers. Qualitative semi-structured interviews were conducted with managers (n = 23) of grocery stores/supermarkets in urban and rural areas across four different states: TN, KY, NC, and NY. Grocery store managers offering online ordering (n = 15) and managers from brick-and-mortar stores without online services (n = 8) participated in the interviews. Three primary themes emerged among managers offering online ordering: (1) order fulfillment challenges, (2) perceived customer barriers, and (3) perceived customer benefits. Among managers at brick-and-mortar locations without online services, four major themes emerged: (1) thoughts on implementing online shopping, (2) COVID-19 pandemic impacts, (3) competition with other stores, and (4) benefits of maintaining brick-and-mortar shopping. This study provides a deeper understanding of retailers’ experience and perceptions of online grocery services among stores authorized to accept SNAP benefits. This perspective is necessary to inform policies and enhance the evolving virtual food marketplace for SNAP customers.
Generation and Characterization of Drug-Resistant Influenza B Viruses Selected In Vitro with Baloxavir Acid
Authors: Saim-Mamoun Amel , Abed Yacine , Carbonneau Julie , Boivin Guy
Published: September 2022

Baloxavir marboxil (BXM) is an antiviral drug that targets the endonuclease of the influenza polymerase acidic (PA) protein. Antiviral resistance, mainly mediated by the I38T PA substitution, readily occurs in both A(H1N1) and A(H3N2) viruses following a single dose of BXM. Influenza B resistance to BXM remains poorly documented. We aimed to generate baloxavir-resistant contemporary influenza B/Yamagata/16/1988- and B/Victoria/2/1987-like viruses by in vitro passages under baloxavir acid (BXA) pressure to identify resistance mutations and to characterize the fitness of drug-resistant variants. Influenza B/Phuket/3073/2013 recombinant virus (rg-PKT13, a B/Yamagata/16/1988-like virus) and B/Quebec/MCV-11/2019 (MCV19, a B/Victoria/2/1987-like isolate) were passaged in ST6GalI-MDCK cells in the presence of increasing concentrations of BXA. At defined passages, viral RNA was extracted for sequencing the PA gene. The I38T PA substitution was selected in MCV19 after six passages in presence of BXA whereas no PA change was detected in rg-PKT13. The I38T substitution increased the BXA IC50 value by 13.7-fold in the MCV19 background and resulted in reduced viral titers compared to the wild type (WT) at early time points in ST6GalI-MDCK and at all time-points in human epithelial cells. By contrast, the I38T substitution had no impact on MCV19 polymerase activity, and this mutation was genetically stable over four passages. In conclusion, our results show a similar pathway of resistance to BXA in influenza B viruses highlighting the major role of the I38T PA substitution and suggest that I38T may differently impact the fitness of influenza variants depending on the viral type, subtype, or lineage.
Dilemmas in the Choice of Adequate Therapeutic Treatment in Patients with Acute Pulmonary Embolism—From Modern Recommendations to Clinical Application
Authors: Lasica Ratko , Asanin Milika , Djukanovic Lazar , Radovanovic Nebojsa , Savic Lidija , Polovina Marija , Stankovic Sanja , Ristic Arsen , Zdravkovic Marija , Lasica Andjelka , Kravic Jelena , Perunicic Jovan
Published: September 2022

Pulmonary thromboembolism is a very common cardiovascular disease, with a high mortality rate. Despite the clear guidelines, this disease still represents a great challenge both in diagnosis and treatment. The heterogeneous clinical picture, often without pathognomonic signs and symptoms, represents a huge differential diagnostic problem even for experienced doctors. The decisions surrounding this therapeutic regimen also represent a major dilemma in the group of patients who are hemodynamically stable at initial presentation and have signs of right ventricular (RV) dysfunction proven by echocardiography and positive biomarker values (pulmonary embolism of intermediate–high risk). Studies have shown conflicting results about the benefit of using fibrinolytic therapy in this group of patients until hemodynamic decompensation, due to the risk of major bleeding. The latest recommendations give preference to new oral anticoagulants (NOACs) compared to vitamin K antagonists (VKA), except for certain categories of patients (patients with antiphospholipid syndrome, mechanical valves, pregnancy). When using oral anticoagulant therapy, special attention should be paid to drug–drug interactions, which can lead to many complications, even to the death of the patient. Special population groups such as pregnant women, obese patients, patients with antiphospholipid syndrome and the incidence of cancer represent a great therapeutic challenge in the application of anticoagulant therapy. In these patients, not only must the effectiveness of the drugs be taken into account, but great attention must be paid to their safety and possible side effects, which is why a multidisciplinary approach is emphasized in order to provide the best therapeutic option.
Use of Nanoparticles to Prevent Resistance to Antibiotics—Synthesis and Characterization of Gold Nanosystems Based on Tetracycline
Authors: Giráldez-Pérez Rosa M. , Grueso Elia M. , Jiménez-Aguayo Raquel , Carbonero Alfonso , González-Bravo Marina , Kuliszewska Edyta , Prado-Gotor Rafael
Published: September 2022

Antimicrobial resistance (AMR) is a serious public health problem worldwide which, according to the World Health Organization (WHO), requires research into new and more effective drugs. In this work, both gold nanoparticles covered with 16-3-16 cationic gemini surfactant (Au@16-3-16) and DNA/tetracycline (DNA/TC) intercalated complexes were prepared to effectively transport tetracycline (TC). Synthesis of the Au@16-3-16 precursor was carried out by using trihydrated gold, adding sodium borohydride as a reducing agent and the gemini surfactant 16-3-16 as stabilizing agent. Circular dichroism and atomic force microscopy techniques were then used to ascertain the optimal R range of the relationship between the concentrations of Au@16-3-16 and the DNA/TC complex (R = CAu@16-3-16/CDNA) that allow the obtainment of stable and compact nanosystems, these characteristics being fundamental for their use as antibiotic transporters. Stability studies over time were carried out for distinct selected Au@16-3-16 and Au@16-3-16/DNA-TC nanoformulations using the ultraviolet–visible spectrophotometry technique, checking their stability for at least one month. In addition, in order to know the charge and size distribution of the nanocomplexes, DLS and zeta potential measurements were performed in the solution. The results showed that the characterized nanosystems were highly charged, stable and of a reduced size (<100 nm) that allows them to cross bacterial membranes effectively (>1 μm). Once the different physicochemical characteristics of the gold nanosystems were measured, Au@16-3-16 and Au@16-3-16/DNA-TC were tested on Escherichia coli and Staphylococcus aureus to study their antibacterial properties and internalization capacity in microbes. Differences in the interaction of the precursors and the compacted nanosystems generated were observed in Gram-positive and Gram-negative bacteria, possibly due to membrane damage or electrostatic interaction with internalization by endocytosis. In the internalization experiments, depending on the treatment application time, the greatest bacterial destruction was observed for all nanoformulations explored at 18 h of incubation. Importantly, the results obtained demonstrate that both new nanosystems based on TC and Au@16-3-16 precursors have optimal antimicrobial properties and would be beneficial for use in patients, avoiding possible side effects.
Role of IL6R Genetic Variants in Predicting Response to Tocilizumab in Patients with Rheumatoid Arthritis
Authors: Sainz Luis , Riera Pau , Moya Patricia , Bernal Sara , Casademont Jordi , Díaz-Torné Cesar , Millán Ana Milena , Park Hye Sang , Lasa Adriana , Corominas Héctor
Published: September 2022

Rheumatoid arthritis (RA) is a prevalent autoimmune disease characterized by chronic arthritis that may lead to irreversible joint damage and significant disability. Patients with RA are commonly treated with Tocilizumab (TCZ), an IL-6 receptor (IL-6R) antagonist, but many patients refractorily respond to this therapy. Identifying genetic biomarkers as predictors of TCZ response could be a key to providing a personalized medicine strategy. We aimed to evaluate whether functional single nucleotide polymorphisms (SNPs) in the IL6R gene could predict TCZ response in patients with RA. We retrospectively included 88 RA patients treated with TCZ. Six SNPs previously described in the IL6R gene (rs12083537, rs11265618, rs4329505, rs2228145, rs4537545, and rs4845625) were genotyped in DNA samples from these patients. Using parametric tests, we evaluated the association between these polymorphisms and clinicopathological features. Responses to treatments were assessed at six months using three variables: a quantitative improvement in Disease activity score including 28 joints (DAS28), a satisfactory European League Against Rheumatism (EULAR) response, and low disease activity (LDA) achievement. The three response variables studied were associated with genetic variant rs4845625, and no association was found with the other five SNPs. Our findings support the potential clinical value of SNPs in the IL6R gene as predictive biomarkers for TCZ response.
L-Serine-Modified Poly-L-Lysine as a Biodegradable Kidney-Targeted Drug Carrier for the Efficient Radionuclide Therapy of Renal Cell Carcinoma
Authors: Katsumi Hidemasa , Kitada Sho , Yasuoka Shintaro , Takashima Rie , Imanishi Tomoki , Tanaka Rina , Matsuura Satoru , Kimura Hiroyuki , Kawashima Hidekazu , Morishita Masaki , Yamamoto Akira
Published: September 2022

In the present study, L-serine (Ser)-modified poly-L-lysine (PLL) was synthesized to develop a biodegradable, kidney-targeted drug carrier for efficient radionuclide therapy in renal cell carcinoma (RCC). Ser-PLL was labeled with 111In/90Y via diethylenetriaminepentaacetic acid (DTPA) chelation for biodistribution analysis/radionuclide therapy. In mice, approximately 91% of the total dose accumulated in the kidney 3 h after intravenous injection of 111In-labeled Ser-PLL. Single-photon emission computed tomography/computed tomography (SPECT/CT) imaging showed that 111In-labeled Ser-PLL accumulated in the renal cortex following intravenous injection. An intrarenal distribution study showed that fluorescein isothiocyanate (FITC)-labeled Ser-PLL accumulated mainly in the renal proximal tubules. This pattern was associated with RCC pathogenesis. Moreover, 111In-labeled Ser-PLL rapidly degraded and was eluted along with the low-molecular-weight fractions of the renal homogenate in gel filtration chromatography. Continuous Ser-PLL administration over five days had no significant effect on plasma creatinine, blood urea nitrogen (BUN), or renal histology. In a murine RCC model, kidney tumor growth was significantly inhibited by the administration of the beta-emitter 90Y combined with Ser-PLL. The foregoing results indicate that Ser-PLL is promising as a biodegradable drug carrier for kidney-targeted drug delivery and efficient radionuclide therapy in RCC.
Is There a Role for Sound in Plants?
Authors: Del Stabile Filippo , Marsili Vittoria , Forti Luca , Arru Laura
Published: September 2022

Plants have long been considered passive, static, and unchanging organisms, but this view is finally changing. More and more knowledge is showing that plants are aware of their surroundings, and they respond to a surprising variety of stimuli by modifying their growth and development. Plants extensively communicate with the world around them, above and below ground. Although communication through mycorrhizal networks and Volatile Organic Compounds has been known for a long time, acoustic perception and communication are somehow a final frontier of research. Perhaps surprisingly, plants not only respond to sound, they actually seem to emit sound as well. Roots emit audible clicks during growth, and sounds are emitted from xylem vessels, although the nature of these acoustic emissions still needs to be clarified. Even more interesting, there is the possibility that these sounds carry information with ecological implications, such as alerting insects of the hydration state of a possible host plant, and technological implications as well. Monitoring sound emissions could possibly allow careful monitoring of the hydration state of crops, which could mean significantly less water used during irrigation. This review summarizes the current knowledge on sound perception communication in plants and illustrates possible implications and technological applications.
Nano-Restoration for Sustaining Soil Fertility: A Pictorial and Diagrammatic Review Article
Authors: El-Ramady Hassan , Brevik Eric C. , Fawzy Zakaria F. , Elsakhawy Tamer , Omara Alaa El-Dein , Amer Megahed , Faizy Salah E.-D. , Abowaly Mohamed , El-Henawy Ahmed , Kiss Attila , Törős Gréta , Prokisch József , Ling Wanting
Published: September 2022

Soil is a real treasure that humans cannot live without. Therefore, it is very important to sustain and conserve soils to guarantee food, fiber, fuel, and other human necessities. Healthy or high-quality soils that include adequate fertility, diverse ecosystems, and good physical properties are important to allow soil to produce healthy food in support of human health. When a soil suffers from degradation, the soil’s productivity decreases. Soil restoration refers to the reversal of degradational processes. This study is a pictorial review on the nano-restoration of soil to return its fertility. Restoring soil fertility for zero hunger and restoration of degraded soils are also discussed. Sustainable production of nanoparticles using plants and microbes is part of the process of soil nano-restoration. The nexus of nanoparticle–plant–microbe (NPM) is a crucial issue for soil fertility. This nexus itself has several internal interactions or relationships, which control the bioavailability of nutrients, agrochemicals, or pollutants for cultivated plants. The NPM nexus is also controlled by many factors that are related to soil fertility and its restoration. This is the first photographic review on nano-restoration to return and sustain soil fertility. However, several additional open questions need to be answered and will be discussed in this work.
DNA Free CRISPR/DCAS9 Based Transcriptional Activation System for UGT76G1 Gene in Stevia rebaudiana Bertoni Protoplasts
Authors: Ghose Asish Kumar , Abdullah Siti Nor Akmar , Md Hatta Muhammad Asyraf , Megat Wahab Puteri Edaroyati
Published: September 2022

The UDP-glycosyltransferase 76G1 (UGT76G1) is responsible for the conversion of stevioside to rebaudioside A. Four single guide RNAs (sgRNAs) were designed from the UGT76G1 proximal promoter region of stevia by using the online-based tool, benchling. The dCas9 fused with VP64 as a transcriptional activation domain (TAD) was produced and purified for the formation of ribonucleoproteins (RNPs) by mixing with the in vitro transcribed sgRNAs. Protoplast yield was the highest from leaf mesophyll of in vitro grown stevia plantlets (3.16 × 106/g of FW) using ES5 (1.25% cellulase R-10 and 0.75% macerozyme R-10). The RNPs were delivered into the isolated protoplasts through the Polyethylene glycol (PEG)-mediated transfection method. The highest endogenous activation of the UGT76G1 gene was detected at 27.51-fold after 24 h of transfection with RNP30 consisting of CRISPR/dCas9-TAD with sgRNA30 and a similar activation level was obtained using RNP18, RNP33, and RNP34, produced using sgRNA18, sgRNA33, and sgRNA34, respectively. Activation of UGT76G1 by RNP18 led to a significant increase in the expression of the rate-limiting enzyme UGT85C2 by 2.37-fold and there was an increasing trend in the expression of UGT85C2 using RNP30, RNP33, and RNP34. Successful application of CRISPR/dCas9-TAD RNP in activating specific genes can avoid the negative integration effects of introduced DNA in the host genome.
Location of Tandem Repeats on Wheat Chromosome 5B and the Breakpoint on the 5BS Arm in Wheat Translocation T7BS.7BL-5BS Using Single-Copy FISH Analysis
Authors: Zhang Wei , Tang Zongxiang , Luo Jie , Li Guangrong , Yang Zujun , Yang Manyu , Yang Ennian , Fu Shulan
Published: September 2022

Wheat (Triticum aestivum L.) is rich in tandem repeats, and this is helpful in studying its karyotypic evolution. Some tandem repeats have not been assembled into the wheat genome sequence. Alignment using the blastn tool in the B2DSC web server indicated that the genomic sequence of 5B chromosome (IWGSC RefSeq v2.1) does not contain the tandem repeat pTa-275, and the tandem repeat (GA)26 distributed throughout the whole 5B chromosome. The nondenaturing fluorescence in situ hybridization (ND-FISH) using the oligonucleotide (oligo) probes derived from pTa-275 and (GA)26 indicated that one signal band of pTa-275 and two signal bands of (GA)26 appeared on the 5B chromosome of Chinese Spring wheat, indicating the aggregative distribution patterns of the two kinds of tandem repeats. Single-copy FISH indicated that the clustering region of pTa-275 and the two clustering regions of (GA)26 were located in ~160–201 Mb, ~153–157 Mb, and ~201–234 Mb intervals, respectively. Using ND-FISH and single-copy FISH technologies, the translocation breakpoint on the 5BS portion of the translocation T7BS.7BL-5BS, which exists widely in north-western European wheat cultivars, was located in the region from 157,749,421 bp to 158,555,080 bp (~0.8 Mb), and this region mainly contains retrotransposons, and no gene was found. The clustering regions of two kinds of tandem repeats on wheat chromosome 5B were determined and this will be helpful to improve the future sequence assembly of this chromosome. The sequence characteristics of the translocation breakpoint on the translocation T7BS.7BL-5BS obtained in this study are helpful to understand the mechanism of wheat chromosome translocation.
Small RNA Differential Expression Analysis Reveals miRNAs Involved in Dormancy Progression in Sweet Cherry Floral Buds
Authors: Soto Esteban , Sanchez Evelyn , Nuñez Carlos , Montes Christian , Rothkegel Karin , Andrade Paola , Prieto Humberto , Almeida Andrea Miyasaka
Published: September 2022

In sweet cherry (Prunus avium), as in other temperate woody perennials, bud dormancy allows for survival in adverse environmental conditions during winter. During this process, environmental signals such as short days and/or low temperatures trigger internal signals that enable buds to become tolerant to the cold. The process involves tracking chilling units up to chilling the requirement fulfillment to resume growth, a transition involving transcriptional regulation, metabolic signaling, and epigenetic-related regulatory events. Massive sequencing of small RNAs was performed to identify miRNAs involved in sweet cherry dormancy by comparing their expression in field (regular seasonal) and controlled non-stop (continuous) chilling conditions. miRNAs highlighted by sequencing were validated using specific stem-loop PCR quantification, confirming expression patterns for known miRNAs such as miR156e, miR166c, miR172d, miR391, miR482c, and miR535b, as well as for newly proposed miRNAs. In silico prediction of the target genes was used to construct miRNA/target gene nodes. In particular, the involvement of the sweet cherry version for the miR156/SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN genes whose expression was opposite in the two conditions suggests their involvement on dormancy regulation in sweet cherry. miRNA levels indicate that the regulation of stress-related genes and hormone synthesis modulates the expression of calcium metabolism and cell development-associated genes. Understanding the regulatory networks involved in sweet cherry dormancy, particularly in the context of miRNA involvement, represents the first step in the development of new agricultural strategies that may help overcome the increasing challenges presented by global climate change.
The Influence of Phytosociological Cultivation and Fertilization on Polyphenolic Content of Menthae and Melissae folium and Evaluation of Antioxidant Properties through In Vitro and In Silico Methods
Authors: Luță Emanuela Alice , Biță Andrei , Moroșan Alina , Mihaiescu Dan Eduard , Ghica Manuela , Mihai Dragoș Paul , Olaru Octavian Tudorel , Deculescu-Ioniță Teodora , Duțu Ligia Elena , Popescu Maria Lidia , Costea Liliana , Nitulescu George Mihai , Lupuliasa Dumitru , Boscencu Rica , Gîrd Cerasela Elena
Published: September 2022

Since medicinal plants are widely used in treating various diseases, phytoconstituents enrichment strategies are of high interest for plant growers. First of all, we investigated the impact of phytosociological cultivation on polyphenolic content (total flavonoids—TFL, and total polyphenols—TPC) of peppermint (Mentha piperita L.) and lemon balm (Melissa officinalis L.) leaves, using spectrophotometric methods. Secondly, the influence of chemical (NPK) and organic (BIO) fertilization on polyphenolic content and plant material quality was also assessed. Dry extracts were obtained from harvested leaves using hydroethanolic extraction solvents for further qualitative and quantitative assessment of phytoconstituents by FT-ICR MS and UHPLC-MS. Furthermore, the antioxidant activity of leaf extracts was determined in vitro using DPPH, ABTS and FRAP methods. Molecular docking simulations were employed to further evaluate the antioxidant potential of obtained extracts, predicting the interactions of identified phytochemicals with sirtuins. The concentration of polyphenols was higher in the plant material harvested from the phytosociological culture. Moreover, the use of BIO fertilizer led to the biosynthesis of a higher content of polyphenols. Higher amounts of phytochemicals, such as caffeic acid, were determined in extracts obtained from phytosociological crops. The antioxidant activity was dependent on polyphenols concentration, more potent inhibition values being observed for the extracts obtained from the phytosociological batches. Molecular docking studies and MM/PBSA calculations revealed that the obtained extracts have the potential to directly activate sirtuins 1, 5 and 6 through several polyphenolic compounds, such as rosmarinic acid, thus complementing the free radical scavenging activity with the potential stimulation of endogenous antioxidant defense mechanisms. In conclusion, growing medicinal plants in phytosociological cultures treated with biofertilizers can have a positive impact on plant material quality, concentration in active constituents and biological activity.
Use of Opuntia ficus-indica Fruit Peel as a Novel Source of Mucilage with Coagulant Physicochemical/Molecular Characteristics
Authors: Otálora Maria , Wilches-Torres Andrea , Lara Carlos , Cifuentes Gabriel , Gómez Castaño Jovanny
Published: September 2022

The peels obtained as a byproduct from the processing of fruits (prickly pears) of the Cactaceae family are a rich source of mucilage, a hydrocolloid biopolymer that may have potential application in water/wastewater treatment as a natural coagulant. In this study, the structural (UPLC-QTOF-MS, FTIR, Raman, NMR, XRD, and zeta potential), morphological (SEM), and thermal (DSC/TGA) characterizations of the mucilage extracted from the peels of Opuntia ficus-indica (OFI) fruits were carried out. UPLC-QTOF-MS results revealed the presence of a branched polymer with an average molecular weight of 0.44 KDa for this mucilage in aqua media. The NMR spectra of mucilage in DMSO-d6 indicated that it seemed well-suited as a coagulant with its typical oligosaccharide structure. FTIR studies confirmed the presence of hydroxyl and carboxyl functional groups in the mucilage, indicating its polyelectrolyte nature that could provide coagulating properties through binding and adsorption mechanisms. Likewise, the zeta potential of −23.63 ± 0.55 mV showed an anionic nature of the mucilage. Power XRD technique evidenced the presence of crystalline poly(glycine-β-alanine), glutamic acid, and syn-whewellite. SEM images revealed an irregular and amorphous morphology with cracks, which are suitable characteristics for adsorption mechanisms. The mucilage exhibited two endothermic transitions, with a decomposition temperature in uronic acid of 423.10 °C. These findings revealed that mucilage obtained from OFI fruit peels has molecular and physicochemical characteristics that are suited to its possible application as a natural coagulant in water/wastewater treatments.
Membranes Based on Polyvinylidene Fluoride and Radiation-Grafted Sulfonated Polystyrene and Their Performance in Proton-Exchange Membrane Fuel Cells
Authors: Golubenko Daniil , Korchagin Oleg , Voropaeva Daria , Bogdanovskaya Vera , Yaroslavtsev Andrey
Published: September 2022

Proton-exchange membranes based on gamma-irradiated films of PVDF and radiation-grafted sulfonated polystyrene with an ion-exchange capacity of 1.8 meq/g and crosslinking degrees of 0 and 3% were synthesized. A solvent-free, environmentally friendly method of styrene grafting from its aqueous emulsion, with a styrene content of only 5 vol.% was used. Energy dispersive X-ray mapping analysis showed that the grafted sulfonated polystyrene is uniformly distributed throughout the membrane thickness. The obtained materials had a proton conductivity up to 132 mS/cm at 80 °C and a hydrogen permeability of up to 5.2 cm2/s at 30 °C, which significantly exceeded similar values for Nafion®-212 membranes. The resulting membranes exhibited a H2/O2 fuel cell peak power density of up to 0.4 W/cm2 at 65 °C. Accelerated stability tests showed that adding a crosslinking agent could significantly increase the stability of the membranes in the fuel cells. The thermal properties and crystallinity of the membranes were investigated through differential scanning calorimetry and X-ray powder diffraction methods. The conductivity, water uptake, and mechanical properties of the membranes (stress–strain curves) were also characterized.
A Molecular Dynamics Simulation of Polymers’ Interactions with Kaolinite (010) Surfaces in Saline Solutions
Authors: Quezada Gonzalo R. , Leiva Williams , Saavedra Jorge H. , Robles Pedro , Gálvez Edelmira , Jeldres Ricardo I.
Published: September 2022

The search for polymers that meet the demands of the water recovery process in mining is a contingent challenge. Both the presence of clays and saline waters can impair water recovery from tailings when conventional flocculants are used. In this work, the adsorption of polyacrylamide (PAM), hydrolyzed polyacrylamide (HPAM), poly(2-acrylamido-2-methyl-1-propane sulfonic acid) (PAMPS), polyacrylic acid (PAA), polyethylene oxide (PEO), and guar gum (GUAR) on a kaolinite surface (010) was investigated using classical molecular dynamics. The results show that the presence of sodium chloride modifies the affinities of the polymers with kaolinite (010). At low salt concentrations, the PAM and GUAR polymers generally show higher adsorption due to the formation of hydrogen bridges. However, the highest adsorptions occur in salt solutions in the presence of HPAM by cationic bridging with sodium ions as a mediator. This high affinity of HPAM is not efficient for flocculation because it re-disperses the particles, but it is promising for the design of new additives produced by grafting HPAM groups onto advanced polymers.
Gandhi and the World of the Hebrew Bible: The Case of Daniel as Satyagrahi
Authors: Noort Ed
Published: September 2022

Among the biblical characters used as examples in developing and explaining satyagraha, Daniel is the most important after Jesus. In Gandhi’s speeches and writings from 1909 to 1946, Daniel served as the ideal satyagrahi both in South Africa and in India. Over time, Daniel received company in the gallery of examples in which Socrates occupied a prominent place. Depending on theme, place, and audience, past and present characters from different traditions and scriptures accompanied Daniel. They represented the development of aspects of satyagraha: nonviolent active resistance as a weapon of the strong, courageous actions as a deliberate choice without excitement, love for the antagonist, preparedness to suffer, and no fear of death. All these aspects are embodied by the Gandhian Daniel. Gandhi emphasized the active role of Daniel as a resister, not the traditional view of the victim of court intrigues. In this paper, I argue that the image of the ideal satyagrahi Daniel could be strengthened by combining the court narratives from the first half and the apocalypses from the second one of the biblical book. The article provides context both for Gandhi’s political and religious practice and for the book of Daniel. The strange world of apocalypses seems to contradict the model of the Gandhian figure Daniel. However, they are crisis literature, and it makes sense to observe how the protagonist and his audience in times of occupation, persecutions, and war ask for guidance. Apocalypses show how Jewish resistance to foreign rule was conceived. The result of the survey is a complex image of competing literatures from roughly the same period and the hands, heads, beliefs, and sufferings behind them. The view of the end of history, a program of nonviolence, and hope in the Daniel apocalypse serve as contrast propaganda to contemporary visions on the violent Maccabean revolt and the Seleucid persecutions. They offer a nonviolent counterweight to the ideology of the state propaganda of the Seleucids. They contradict the historiographic idealization of the Maccabean revolt and its armed resistance.
Political Action of the Catholic Hierarchy and the Use of Religion in Political Organizations (Peru, 1920–2021): Evidence and Long-Term Analysis
Authors: Armas Asín Fernando
Published: September 2022

This article presents the characteristics that the Catholic hierarchy has displayed over the course of a century in the political arena, as well as the importance that religious elements have exercised over the legitimacy of some parties and governments in Peru. It specifically analyzes the clergy and their relation with the State, as well as interpreting how they understood their participation and influence in local politics. In addition, parties and regimes over the course of a century are analyzed, emphasizing the presidential election campaigns of 1990 and 2021, in order to discuss the limits that political organizations have experienced in monopolizing religious representations. This study will help to better understand the nature of Catholicism in Latin American politics.