bluuebunny/crossref_metadata_2025 huggingface dataset:
Two Combinatorial Algorithms for the Constrained Assignment Problem with Bounds and Penalties
Authors: Hu Guojun , Lichen Junran , Pan Pengxiang
Published: December 2023

In the paper, we consider a generalization of the classical assignment problem, which is called the constrained assignment problem with bounds and penalties (CA-BP). Specifically, given a set of machines and a set of independent jobs, each machine has a lower and upper bound on the number of jobs that can be executed, and each job must be either executed on some machine with a given processing time or rejected with a penalty that we must pay for. No job can be executed on more than one machine. We aim to find an assignment scheme for these jobs that satisfies the constraints mentioned above. The objective is to minimize the total processing time of executed jobs as well as the penalties from rejected jobs. The CA-BP is related to some practical applications such as edge computing, which involves selecting tasks and processing them on the edge servers of an internet network. As a result, a motivation of this study is to improve the efficiency of internet networks by limiting the lower bound of the number of objects processed by each edge server. Our main contribution is modifying the previous network flow algorithms to satisfy the lower capacity constraints, for which we design two exact combinatorial algorithms to solve the CA-BP. Our methodologies and results bring novel perspectives into other research areas related to the assignment problem.
A New Solution to the Fractional Black–Scholes Equation Using the Daftardar-Gejji Method
Authors: Sugandha Agus , Rusyaman Endang , Sukono  , Carnia Ema
Published: December 2023

The main objective of this study is to determine the existence and uniqueness of solutions to the fractional Black–Scholes equation. The solution to the fractional Black–Scholes equation is expressed as an infinite series of converging Mittag-Leffler functions. The method used to discover the new solution to the fractional Black–Scholes equation was the Daftardar-Geiji method. Additionally, the Picard–Lindelöf theorem was utilized for the existence and uniqueness of its solution. The fractional derivative employed was the Caputo operator. The search for a solution to the fractional Black–Scholes equation was essential due to the Black–Scholes equation’s assumptions, which imposed relatively tight constraints. These included assumptions of a perfect market, a constant value of the risk-free interest rate and volatility, the absence of dividends, and a normal log distribution of stock price dynamics. However, these assumptions did not accurately reflect market realities. Therefore, it was necessary to formulate a model, particularly regarding the fractional Black–Scholes equation, which represented more market realities. The results obtained in this paper guaranteed the existence and uniqueness of solutions to the fractional Black–Scholes equation, approximate solutions to the fractional Black–Scholes equation, and very small solution errors when compared to the Black–Scholes equation. The novelty of this article is the use of the Daftardar-Geiji method to solve the fractional Black–Scholes equation, guaranteeing the existence and uniqueness of the solution to the fractional Black–Scholes equation, which has not been discussed by other researchers. So, based on this novelty, the Daftardar-Geiji method is a simple and effective method for solving the fractional Black–Scholes equation. This article presents some examples to demonstrate the application of the Daftardar-Gejji method in solving specific problems.
Critical Analysis of Beta Random Variable Generation Methods
Authors: Almaraz Luengo Elena , Gragera Carlos
Published: December 2023

The fast generation of values of the beta random variable is a subject of great interest and multiple applications, ranging from purely mathematical and statistical ones to applications in management and production, among others. There are several methods for generating these values, with one of the essential points for their design being the selection of random seeds. Two interesting aspects converge here: the use of sequences as inputs (and the need for them to verify properties such as randomness and uniformity, which are verified through statistical test suites) and the design of the algorithm for the generation of the variable. In this paper, we analyse, in detail, the algorithms that have been developed in the literature, both from a mathematical/statistical and computational point of view. We also provide empirical development using R software, which is currently in high demand and is one of the main novelties with respect to previous comparisons carried out in FORTRAN. We establish which algorithms are more efficient and in which contexts, depending on the different values of the parameters, allowing the user to determine the best method given the experimental conditions.
Continuum and Molecular Modeling of Chemical Vapor Deposition at Nano-Scale Fibrous Substrates
Authors: Barua Himel , Povitsky Alex
Published: December 2023

Chemical vapor deposition (CVD) is a common industrial process that incorporates a complex combination of fluid flow, chemical reactions, and surface deposition. Understanding CVD processes requires rigorous and costly experimentation involving multiple spatial scales, from meters to nanometers. The numerical modeling of deposition over macro-scale substrates has been conducted in the literature and results show compliance with experimental data. For smaller-scale substrates, where the corresponding Knudsen number is larger than zero, continuum modeling does not provide accurate results, which calls for the implementation of molecular-level modeling techniques. In the current study, the finite-volume method (FVM) and Direct Simulation Monte Carlo (DSMC) method were combined to model the reactor-scale flow with CVD around micro- and nano-scale fibers. CVD at fibers with round cross-sections was modeled in the reactor, where fibers were oriented perpendicularly with respect to the feedstock gas flow. The DSMC method was applied to modeling flow around the matrix of nano-scale circular individual fibers. Results show that for smaller diameters of individual fibers with the same filling ratio, the residence time of gas particles inside the fibrous media reduces, and, consequently, the amount of material surface deposition decreases. The sticking coefficient on the fibers’ surface plays an important role; for instance, increasing the sticking coefficient from 20% to 80% will double the deposition rate.
Understanding Bidirectional Water Transport across Bronchial Epithelial Cell Monolayers: A Microfluidic Approach
Authors: Zajac Miroslaw , Jakiela Slawomir , Dolowy Krzysztof
Published: December 2023

Deciphering the dynamics of water transport across bronchial epithelial cell monolayers is pivotal for unraveling respiratory physiology and pathology. In this study, we employ an advanced microfluidic system to explore bidirectional water transport across 16HBE14σ bronchial epithelial cells. Previous experiments unveiled electroneutral multiple ion transport, with chloride ions utilizing transcellular pathways and sodium ions navigating both paracellular and transcellular routes. Unexpectedly, under isoosmotic conditions, rapid bidirectional movement of Na+ and Cl− was observed, leading to the hypothesis of a substantial transport of isoosmotic solution (145 mM NaCl) across cell monolayers. To validate this conjecture, we introduce an innovative microfluidic device, offering a 500-fold sensitivity improvement in quantifying fluid flow. This system enables the direct measurement of minuscule fluid volumes traversing cell monolayers with unprecedented precision. Our results challenge conventional models, indicating a self-regulating mechanism governing water transport that involves the CFTR channel and anion exchangers. In healthy subjects, equilibrium is achieved at an apical potential of Δφap = −30 mV, while subjects with cystic fibrosis exhibit modulation by an anion exchanger, reaching equilibrium at [Cl] = 67 mM in the airway surface liquid. This nuanced electrochemical basis for bidirectional water transport in bronchial epithelia sheds light on physiological intricacies and introduces a novel perspective for understanding respiratory conditions.
Treatment of Stainless Steel Rinse Waters Using Non-Dispersive Extraction and Strip Dispersion Membrane Technology
Authors: Alguacil Francisco Jose , Robla Jose Ignacio
Published: December 2023

The extraction of Fe(III), Cr(III), and Ni(II) from stainless steel rinse water using non-dispersive extraction and strip dispersion membrane technology was carried out in a microporous hydrophobic hollow-fibre module contactor. The fibres were of polypropylene, whereas the organic extractant DP8R (bis(2-ethylhexyl) phosphoric acid) diluted in ExxsolD100 was used as the carrier phase. The rinse water containing the three elements was passed through the tube side, and the pseudo-emulsion formed by the organic phase of DP8R in Exxol D100 and an acidic strip solution were passed through the shell side in a counter-current operation; thus, a unique hollow fibre module was used for extraction and stripping. In non-dispersive extraction and strip dispersion technology, the stripping solution was dispersed into the organic membrane solution in a vessel with an adequate mixing device (impeller) designed to form strip dispersion. This pseudo-emulsion was circulated from the vessel to the membrane module to provide a constant supply of the organic phase to the membrane pores. Different hydrodynamic and chemical variables, such as variation in feed and pseudo-emulsion flow rates, strip phase composition, feed phase pH, and extractant concentration in the organic phase, were investigated. Mass transfer coefficients were estimated from the experimental data. It was possible to separate and concentrate the metals present in the rinse water using the non-dispersive extraction and strip dispersion technique.
Oleic Acid Metabolism in Response to Glucose in C. elegans
Authors: Vieira Andre F. C. , Xatse Mark A. , Murray Sofi Y. , Olsen Carissa Perez
Published: December 2023

A key response to glucose stress is an increased production of unsaturated fatty acids to balance the increase in saturated fatty acids in the membrane. The C. elegans homolog of stearoyl-CoA desaturase, FAT-7, introduces the first double bond into saturated C18 fatty acids yielding oleic acid, and is a critical regulatory point for surviving cold and glucose stress. Here, we incorporated 13C stable isotopes into the diet of nematodes and quantified the 13C-labelled fatty acid using GC-MS and HPLC/MS-MS to track its metabolic response to various concentrations of glucose. Previous work has analyzed the membrane composition of C. elegans when responding to mild glucose stress and showed few alterations in the overall fatty acid composition in the membrane. Here, in nematodes exposed to higher concentrations of glucose, a specific reduction in oleic acid and linoleic acid was observed. Using time courses and stable isotope tracing, the response of fatty acid metabolism to increasing levels of glucose stress is characterized, revealing the funneling of monounsaturated fatty acids to preserve the abundance of polyunsaturated fatty acids. Taken together, higher levels of glucose unveil a specific reduction in oleic and linolenic acid in the metabolic rewiring required to survive glucose stress.
Olive Oil Polyphenols Improve HDL Cholesterol and Promote Maintenance of Lipid Metabolism: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Zupo Roberta , Castellana Fabio , Crupi Pasquale , Desantis Addolorata , Rondanelli Mariangela , Corbo Filomena , Clodoveo Maria Lisa
Published: December 2023

In 2011, the European Food Safety Authority (EFSA) accorded a health claim to olive oil polyphenols in that they protected LDL particles from oxidative damage. However, limited scientific evidence has so far failed to confer any claim of function on the maintenance of normal lipid metabolism. We performed a systematic review and meta-analysis of human RCTs, evaluating the effect of olive oil polyphenol administration on lipid profiles. Previous literature was acquired from six electronic databases until June 2023. A total of 75 articles were retrieved and screened for inclusion criteria, which resulted in the selection of 10 RCTs that evaluated the effect of daily exposure to olive oil polyphenols on serum lipids in adults. Meta-analyses were built by tertiles of outcomes, as follows: low (0–68 mg/kg), medium (68–320 mg/kg), and high (320–600 mg/kg) polyphenols for HDL and LDL cholesterol (HDL-C and LDL-C, respectively), and low (0–59.3 mg/kg), medium (59.3–268 mg/kg), and high (268–600 mg/kg) polyphenols for total cholesterol (TC). The study protocol was registered on PROSPERO (registration code: CRD42023403383). The study design was predominantly cross-over (n = 8 of 10) but also included parallel (n = 2 of 10). The study population was predominantly European and healthy. Daily consumption of olive oil polyphenols did not affect TC levels and only slightly significantly reduced LDL-C, with WMD statistically significant only for high daily consumption of olive oil polyphenols (WMD −4.28, 95%CI −5.78 to −2.77). Instead, our data found a statistically significant HDL-C enhancing effect (WMD pooled effect model: 1.13, 95%CI 0.45; 1.80, heterogeneity 38%, p = 0.04) with WMD by daily exposure level showing a statistically significant improvement effect for low (WMD 0.66, 95%CI 0.10–1.23), medium (WMD 1.36, 95%CI 0.76–1.95), and high (WMD 1.13, 95%CI 0.45–1.80) olive oil polyphenol consumptions. Olive oil polyphenols contribute toward maintaining lipid metabolism. Thus, food labeling regulations should stress this health feature of olive oil, whereby a declaration of the olive oil polyphenol content should be added to products on the market. Consumers need to be aware of the quality and possible health effects of any products they consume, and enforcement of nutrition labels offers the best way of providing this information.
Biological Control of the Plant Pathogens
Authors: Borriss Rainer , Keswani Chetan
Published: December 2023

(This article belongs to the Special Issue Biological Control of the Plant Pathogens [...]
Petrogenesis and Tectonic Setting of Early Cretaceous A-Type Granite from the Southern Great Xing’an Range, Northeastern China: Geochronological, Geochemical, and Hf Isotopic Evidence
Authors: Ran Xiangjin , Wang Xi , Sun Zhenming
Published: December 2023

The southern Great Xing’an Range is located in the eastern Central Asian Orogenic Belt, where voluminous igneous rocks developed during the Late Mesozoic period. The east slope of the southern Great Xing’an Range has been the topic of numerous debates on the level of influence of the Mongol-Okhotsk and the Paleo-Pacific regimes in the Late Mesozoic period. Therefore, this area is a suitable region in which to study the temporal changes in magma sources and tectono-magmatic evolution. In this paper, whole-rock geochemical data, zircon U-Pb geochronology, and zircon Hf isotope studies were carried out on the granitoids in the east slope area of the southern Great Xing’an Range. LA-ICP-MS zircon U-Pb dating revealed the ages of four granitoid samples: 135.0 ± 0.6 Ma, 130.7 ± 1.4 Ma, 130.4 ± 1.0 Ma, and 127.6 ± 0.8 Ma, respectively. The Hf isotope values 176Hf/177Hf = 0.282751–0.283015, εHf (t) = +2.0~+11.5, and T2DM = 583~1442 Ma suggest that the magma was generated by partial melting of Meso- and Neoproterozoic accreted and thickened low crust. The whole-rock geochemical data implied that these granitoids are A-type granite and their formation is closely linked to the subduction of the Paleo-Pacific Ocean plate. These geochemical, isotopic, and geochronological data suggest that the Early Cretaceous magmatism in the east slope area of the southern Great Xing’an Range formed in an extensional back-arc tectonic setting associated with the slab roll-back of the Paleo-Pacific plate subduction.
In Vitro Antiviral Activity of Hyperbranched Poly-L-Lysine Modified by L-Arginine against Different SARS-CoV-2 Variants
Authors: Fiori Federico , Cossu Franca Lucia , Salis Federica , Carboni Davide , Stagi Luigi , De Forni Davide , Poddesu Barbara , Malfatti Luca , Khalel Abbas , Salis Andrea , Casula Maria Francesca , Anedda Roberto , Lori Franco , Innocenzi Plinio
Published: December 2023

The emergence of SARS-CoV-2 variants requires close monitoring to prevent the reoccurrence of a new pandemic in the near future. The Omicron variant, in particular, is one of the fastest-spreading viruses, showing a high ability to infect people and evade neutralization by antibodies elicited upon infection or vaccination. Therefore, the search for broad-spectrum antivirals that can inhibit the infectious capacity of SARS-CoV-2 is still the focus of intense research. In the present work, hyperbranched poly-L-lysine nanopolymers, which have shown an excellent ability to block the original strain of SARS-CoV-2 infection, were modified with L-arginine. A thermal reaction at 240 °C catalyzed by boric acid yielded Lys-Arg hyperbranched nanopolymers. The ability of these nanopolymers to inhibit viral replication were assessed for the original, Delta, and Omicron strains of SARS-CoV-2 together with their cytotoxicity. A reliable indication of the safety profile and effectiveness of the various polymeric compositions in inhibiting or suppressing viral infection was obtained by the evaluation of the therapeutic index in an in vitro prevention model. The hyperbranched L-arginine-modified nanopolymers exhibited a twelve-fold greater therapeutic index when tested with the original strain. The nanopolymers could also effectively limit the replication of the Omicron strain in a cell culture.
Effects of the Electrical Properties of SnO2 and C60 on the Carrier Transport Characteristics of p-i-n-Structured Semitransparent Perovskite Solar Cells
Authors: Pham Hoang Minh , Naqvi Syed Dildar Haider , Tran Huyen , Tran Hung Van , Delda Jonabelle , Hong Sungjun , Jeong Inyoung , Gwak Jihye , Ahn SeJin
Published: December 2023

Recently, metal halide perovskite-based top cells have shown significant potential for use in inexpensive and high-performance tandem solar cells. In state-of-the-art p-i-n perovskite/Si tandem devices, atomic-layer-deposited SnO2 has been widely used as a buffer layer in the top cells because it enables conformal, pinhole-free, and highly transparent buffer layer formation. In this work, the effects of various electrical properties of SnO2 and C60 layers on the carrier transport characteristics and the performance of the final devices were investigated using a numerical simulation method, which was established based on real experimental data to increase the validity of the model. It was found that the band alignment at the SnO2/C60 interface does, indeed, have a significant impact on the electron transport. In addition, as a general design rule, it was suggested that at first, the conduction band offset (CBO) between C60 and SnO2 should be chosen so as not to be too negative. However, even in a case in which this CBO condition is not met, we would still have the means to improve the electron transport characteristics by increasing the doping density of at least one of the two layers of C60 and/or SnO2, which would enhance the built-in potential across the perovskite layer and the electron extraction at the C60/SnO2 interface.
Middle Meningeal Artery Embolization versus Surgery in Patients with Chronic Subdural Hematoma—No More Fence Sitting?
Authors: Sila Dalibor , Casnati Francisco Luis , Vojtková Mária , Kirsch Philipp , Rath Stefan , Charvát František
Published: December 2023

Background: Endovascular treatment of patients with chronic subdural hematoma using middle meningeal artery (MMA) embolization could become an alternative to surgical hematoma evacuation. The aim of the study was to compare methods and identify parameters to help determine the correct treatment modality. Methods: We retrospectively reviewed 142 cases conducted internally; 78 were treated surgically and 64 were treated using MMA embolization. We analyzed the treatment failure rate and complications, and using a binary logistic regression model, we identified treatment failure risk factors. Results: We found a comparable treatment failure rate of 23.1% for the surgery group and 21.9% for the MMA embolization group. However, in the MMA embolization group, 11 cases showed treatment failure due to early neurological worsening with a need for concomitant surgery. We also found a recurrence of hematoma in 15.4% of cases in the surgery group and 6.3% of cases in the MMA embolization group. Conclusion: Both modalities have their advantages; however, correct identification is crucial for treatment success. According to our findings, hematomas with a maximal width of <18 mm, a midline shift of <5 mm, and no acute or subacute (hyperdense) hematoma could be treated with MMA embolization. Hematomas with a maximal width of >18 mm, a midline shift of >5 mm, and no membranous segmentation could have better outcomes after surgical treatment.
Acute Effects of Naturally Occurring Guayusa Tea and Nordic Lion’s Mane Extracts on Cognitive Performance
Authors: La Monica Michael B. , Raub Betsy , Ziegenfuss Ethan J. , Hartshorn Shelley , Grdic Jodi , Gustat Ashley , Sandrock Jennifer , Ziegenfuss Tim N.
Published: December 2023

The aim of this study was to assess the effects of guayusa extract and Nordic Lion’s Mane (LM) on cognition. Using a randomized, double-blind, placebo-controlled, crossover design, we examined the effects of a single dose of 650 mg guayusa extract (AMT: AmaTea® Max) vs. 1 g Nordic-grown Lion’s Mane (LM) vs. placebo (PL). Participants attended three testing visits consisting of neuropsychological tests (Go/No-go, N-Back, and Serial 7 s tasks) assessing performance, subjective assessments of cognitive perception, and vital signs. Each assessment was measured at baseline (pre-ingestion) and 1 and 2 h post ingestion. AMT significantly (p ≤ 0.05) improved the number of attempts during Serial 7s, total score, number of correct responses, total number of responses, and reaction time during N-Back and improved Go stimulus reaction time, but it reduced the percentage of correct responses in the No-go stimulus response during Go/No-go. LM significantly (p ≤ 0.05) improved the number of attempts during Serial 7s and reaction time during N-Back and improved Go stimulus reaction time in Go/No-go. AMT improved mental clarity, focus, concentration, mood, and productivity at 1 and 2 h (p < 0.05); the ability to tolerate stress at 1 h; and had greater ratings than LM and PL for mental clarity, focus, concentration, and productivity. PL improved focus and concentration at 1 h from baseline (p ≤ 0.05). AMT and LM improved subjective ratings of “happiness compared to peers” and “getting the most out of everything” (p < 0.05); however, this occurred earlier in LM (i.e., 1 h post ingestion). AMT uniquely elevated blood pressure from baseline. AMT significantly improved cognitive performance and self-perceived cognitive indices of affect over a 2 h period and perceptions of happiness 2 h post ingestion. In comparison, LM helped improve working memory, complex attention, and reaction time 2 h post ingestion and perceptions of happiness over a 2 h period.
Weight Management during Pregnancy and the Postpartum Period in Women with Gestational Diabetes Mellitus: A Systematic Review and Summary of Current Evidence and Recommendations
Authors: Huang Jing , Wu Yi , Li Hua , Cui Hangyu , Zhang Qi , Long Tianxue , Zhang Yiyun , Li Mingzi
Published: December 2023

Background: Weight management during pregnancy and the postpartum period is an important strategy that can be utilized to reduce the risk of short- and long-term complications in women with gestational diabetes mellitus (GDM). We conducted a systematic review to assess and synthesize evidence and recommendations on weight management during pregnancy and the postpartum period in women with GDM to provide evidence-based clinical guidance. Methods: Nine databases and eighteen websites were searched for clinical decisions, guidelines, recommended practices, evidence summaries, expert consensus, and systematic reviews. Results: A total of 12,196 records were retrieved and fifty-five articles were included in the analysis. Sixty-nine pieces of evidence were summarized, sixty-two of which focused on pregnancy, including benefits, target population, weight management goals, principles, weight monitoring, nutrition assessment and counseling, energy intake, carbohydrate intake, protein intake, fat intake, fiber intake, vitamin and mineral intake, water intake, dietary supplements, sugar-sweetened beverages, sweeteners, alcohol, coffee, food safety, meal arrangements, dietary patterns, exercise assessment and counseling, exercise preparation, type of exercise, intensity of exercise, frequency of exercise, duration of exercise, exercise risk prevention, and pregnancy precautions, and seven focused on the postpartum period, including target population, benefits, postpartum weight management goals, postpartum weight monitoring, dietary recommendations, exercise recommendations, and postpartum precautions. Conclusions: Healthcare providers can develop comprehensive pregnancy and postpartum weight management programs for women with GDM based on the sixty-nine pieces of evidence. However, because of the paucity of evidence on postpartum weight management in women with GDM, future guidance documents should focus more on postpartum weight management in women with GDM.
How to Manage the Suffering of the Patient and the Family in the Final Stage of Life: A Qualitative Study
Authors: García-Navarro E. Begoña , Garcia Navarro Sonia , Cáceres-Titos María José
Published: December 2023

Background: The end of life and death have changed from being issues managed within the family, assumed as part of life, to occur within health institutions for the majority of patients. The amount of patients dying at home has decreased, and the roles of families and communities in death and dying have become involuted, threatening related traditions and knowledge. As a result, a need to promote the end of life at home in this new self-serving society has arisen. In that context, the main objective of this study was to find out what patients and their families need during the end-of-life process in order to feel effectively accompanied at this time. Methods: With that objective, a descriptive qualitative study was conducted via the content analysis of data from semi-structured interviews and focus groups. This research adhered to the COREQ guidelines. The sample consisted of 36 informants selected via intentional sampling of family members and patients integrated into the Comprehensive Palliative Care Process (PAI Paliativos). Results: The results suggest the existence of several common needs such as communication and presence, including the conspiracy of silence as an important factor generating suffering for both. However, there are specific needs such as autonomy, dignity, and respect for patients, which must be taken into account. Conclusions: The results of this study will allow us to establish intervention strategies for effective accompaniment of patients’ family members at the end of life and the avoidance of ethnocentrism in this process. This study was retrospectively registered with the (nursrep-1194226) on the (21 April 2023).
Smart Responsive and Controlled-Release Hydrogels for Chronic Wound Treatment
Authors: Jia Xintao , Dou Zixuan , Zhang Ying , Li Fanqin , Xing Bin , Hu Zheming , Li Xin , Liu Zhongyan , Yang Wenzhuo , Liu Zhidong
Published: December 2023

Chronic wounds are a major health challenge that require new treatment strategies. Hydrogels are promising drug delivery systems for chronic wound healing because of their biocompatibility, hydration, and flexibility. However, conventional hydrogels cannot adapt to the dynamic and complex wound environment, which involves low pH, high levels of reactive oxygen species, and specific enzyme expression. Therefore, smart responsive hydrogels that can sense and respond to these stimuli are needed. Crucially, smart responsive hydrogels can modulate drug release and eliminate pathological factors by changing their properties or structures in response to internal or external stimuli, such as pH, enzymes, light, and electricity. These stimuli can also be used to trigger antibacterial responses, angiogenesis, and cell proliferation to enhance wound healing. In this review, we introduce the synthesis and principles of smart responsive hydrogels, describe their design and applications for chronic wound healing, and discuss their future development directions. We hope that this review will inspire the development of smart responsive hydrogels for chronic wound healing.
Investigation on Electrospun and Solvent-Casted PCL-PLGA Blends Scaffolds Embedded with Induced Pluripotent Stem Cells for Tissue Engineering
Authors: Rosalia Mariella , Giacomini Martina , Tottoli Erika Maria , Dorati Rossella , Bruni Giovanna , Genta Ida , Chiesa Enrica , Pisani Silvia , Sampaolesi Maurilio , Conti Bice
Published: December 2023

The design, production, and characterisation of tissue-engineered scaffolds made of polylactic-co-glycolic acid (PLGA), polycaprolactone (PCL) and their blends obtained through electrospinning (ES) or solvent casting/particulate leaching (SC) manufacturing techniques are presented here. The polymer blend composition was chosen to always obtain a prevalence of one of the two polymers, in order to investigate the contribution of the less concentrated polymer on the scaffolds’ properties. Physical–chemical characterization of ES scaffolds demonstrated that tailoring of fibre diameter and Young modulus (YM) was possible by controlling PCL concentration in PLGA-based blends, increasing the fibre diameter from 0.6 to 1.0 µm and reducing the YM from about 22 to 9 MPa. SC scaffolds showed a “bubble-like” topography, caused by the porogen spherical particles, which is responsible for decreasing the contact angles from about 110° in ES scaffolds to about 74° in SC specimens. Nevertheless, due to phase separation within the blend, solvent-casted samples displayed less reproducible properties. Furthermore, ES samples were characterised by 10-fold higher water uptake than SC scaffolds. The scaffolds suitability as iPSCs culturing support was evaluated using XTT assay, and pluripotency and integrin gene expression were investigated using RT-PCR and RT-qPCR. Thanks to their higher wettability and appropriate YM, SC scaffolds seemed to be superior in ensuring high cell viability over 5 days, whereas the ability to maintain iPSCs pluripotency status was found to be similar for ES and SC scaffolds.
A Multivariate Meta-Analysis for Optimizing Cell Counts When Using the Mechanical Processing of Lipoaspirate for Regenerative Applications
Authors: Zinger Gershon , Kepes Nia , Kenett Ron , Peyser Amos , Sharon-Gabbay Racheli
Published: December 2023

Lipoaspirate has become the preferred source for regenerative cells. The mechanical processing of lipoaspirate has advantages over enzymatic processing but has a lower yield of regenerative cells. A review of the literature shows different techniques of extraction, but the ideal method or combination has not been determined. Methods: A comprehensive literature search was focused on the mechanical processing of lipoaspirate, without the use of enzymes. Data from the articles were integrated by utilizing a multivariate meta-analysis approach and used to create a statistical-based predictive model for a combination of multiple variables. Results: Starting with 10,000 titles, 159 articles were reviewed, and 6 met the criteria for inclusion and exclusion. The six studies included data on 117 patients. Sixteen factors were analyzed and six were identified as significant. The predictive profilers indicated that the optimal combination to maximize the cell yield was: a centrifuge force of 2000× g, a centrifuge time of 10 min, a cannula diameter of 2 mm, and an intra-syringe number of passes of 30. The optimal patient factors were a higher BMI and younger age. Conclusions: The novelty of the method used here was in combining data across different studies to understand the effect of the individual factors and in the optimization of their combination for mechanical lipoaspirate processing.
Design of the Light Source Layout Optimization Strategy Based on Region Partition and Pre-Bias Compensation for Indoor Visible Light Communication Systems
Authors: Zhao Qiong , Zhang Weilin , Fan Jiacheng , Deng Lijun
Published: December 2023

Optimization of the light source layout is an important issue for indoor visible light communication systems, as it affects the received optical power distribution and user perception. In this paper, we propose a local optimization strategy for the light source layout that balances optimization effectiveness with optimization efficiency. First, we divide the optimization region into multiple sub-regions with different sizes and optimization priorities, where the sizes and optimization priorities of the individual sub-regions are determined based on the effect minimization principle among the sub-regions. We then calculate the pre-bias factor based on the equivalent mapping, which can compensate for the effect of the light sources in the latter optimized sub-region on the source layout optimization in the current sub-region. Finally, we search for the coordinate of a single light source for each sub-region using the variance of the squared distance between the projection of the light source on the receiving plane and the received point as a fitness function. Simulation results show that the proposed optimization strategy performs well when the vertical distance between the ceiling and the receiving plane is not less than 2.85 m.
Effects of Low Light after Heading on the Yield of Direct Seeding Rice and Its Physiological Response Mechanism
Authors: Ma Peng , Zhou Lin , Liao Xue-Huan , Zhang Ke-Yuan , Aer Li-Se , Yang Er-Luo , Deng Jun , Zhang Rong-Ping
Published: December 2023

As a photophilous plant, rice is susceptible to low-light stress during its growth. The Sichuan Basin is a typical low-light rice-producing area. In this study, eight rice varieties with different shade tolerances were studied from 2021 to 2022. The physiological adaptability and yield formation characteristics of rice were studied with respect to photosynthetic physiological characteristics and dry matter accumulation characteristics, and the response mechanism of rice to low light stress was revealed. The results showed that the shading treatment significantly increased the chlorophyll a, chlorophyll b, and total chlorophyll contents in the leaves of direct-seeded rice after heading, and the total chlorophyll content increased by 1.68–29.70%. Nitrate reductase (NR) activity first increased and then decreased under each treatment, and the shading treatment reduced the NR activity of direct-seeded rice. Compared to the control treatment, the peroxidase (POD) activity of each variety increased from 7 to 24 d after the shading treatment. The transketolase (TK) activity in direct-seeded hybrid rice increased under low light stress. Compared with the control, shading treatment significantly reduced the aboveground dry matter, grain number per panicle, and seed setting rate of direct-seeded rice at the full heading stage and maturity stage, thus reducing the yield of direct-seeded rice by 26.10–34.11%. However, under the shading treatment, Zhenliangyou 2018 and Jingliangyou 534 maintained higher chlorophyll content and related enzyme activities, accumulated more photosynthetic products, and reduced yield. In general, Zhenliangyou 2018 and Jingliangyou 534 still had a yield of 7.06–8.33 t·hm−2 under low light. It indicated that Zhenliangyou 2018 and Jingliangyou 534 had better stability and stronger tolerance to weak light stress and had a higher yield potential in weak light areas such as Sichuan.
Rosmarinic Acid and Flavonoids of the Seagrass Zostera noltei: New Aspects on Their Quantification and Their Correlation with Sunlight Exposure
Authors: Casal-Porras Isabel , Muñoz Kimberly , Ortega María J. , Brun Fernando G. , Zubía Eva
Published: December 2023

Seagrasses are plants adapted to the marine environment that inhabit shallow coastal waters, where they may be exposed to direct sunlight during low tides. These plants have photoprotection mechanisms, which could include the use of phenolic secondary metabolites. In this study, rosmarinic acid (RA) and the flavonoids of Zostera noltei from the Bay of Cadiz (Spain) have been analyzed, first to define suitable conditions of leaves (i.e., fresh, dried, or frozen) for quantitative analysis, and then to explore the potential correlation between the phenolic profile of the leaves and sunlight exposure using an in situ experimental approach. Compared with fresh leaves, the contents of RA and flavonoids were significantly lower in air-dried and freeze-dried leaves. Freezing caused highly variable effects on RA and did not affect to flavonoid levels. On the other hand, the content of RA was significantly higher in plants that emerged during low tides than in plants permanently submerged, while plants underneath an artificial UV filter experienced a progressive reduction in RA content. However, the major flavonoids did not show a clear response to sunlight exposure and were unresponsive to diminished UV incidence. The results showed a positive correlation of RA with direct sunlight and UV exposure of leaves, suggesting that this compound contributes to the photoprotection of Z. noltei.
Jasmonate: A Hormone of Primary Importance for Temperature Stress Response in Plants
Authors: Wang Meiling , Fan Xiulan , Ding Fei
Published: December 2023

Temperature is a critical environmental factor that plays a vital role in plant growth and development. Temperatures below or above the optimum ranges lead to cold or heat stress, respectively. Temperature stress retards plant growth and development, and it reduces crop yields. Jasmonates (JAs) are a class of oxylipin phytohormones that play various roles in growth, development, and stress response. In recent years, studies have demonstrated that cold and heat stress affect JA biosynthesis and signaling, and JA plays an important role in the response to temperature stress. Recent studies have provided a large body of information elucidating the mechanisms underlying JA-mediated temperature stress response. In the present review, we present recent advances in understanding the role of JA in the response to cold and heat stress, and how JA interacts with other phytohormones during this process.
3‴-O-Foliamenthoyl-Rutin, a New Flavonoid Glycoside from the Roots of Nymphoides peltata
Authors: Kim Tae-Young , Lee Bum Soo , Jo Beom-Geun , Heo Seong Pil , Keem Min-Ji , Kwon Taek-Hwan , Kim Su-Nam , Kim Ki Hyun , Yang Min Hye
Published: December 2023

Nymphoides peltata (Menyanthaceae) has been used as a medicinal herb in traditional medicines to treat conditions such as strangury, polyuria, swelling, and as a diuretic and antipyretic. In our ongoing research to discover novel structural and/or biological natural products in natural resources, five flavonoids, quercetin (1), quercitrin (2), isoquercetin (3), quercetin-3-O-vicianoside (4), and rutin (5), as well as a new flavonoid glycoside, 3‴-O-foliamenthoyl-rutin (6), were isolated from the MeOH extract of N. peltata roots. The chemical structure of the new compound (6) was determined by analyzing 1D and 2D NMR spectra and high-resolution (HR) electrospray ionization mass spectroscopy (ESIMS), along with a chemical reaction. The wound-healing activities of the isolated compounds (1–6) were evaluated using a HaCaT cell scratch test. Among the isolates, isoquercetin (3), quercetin-3-O-vicianoside (4), and 3‴-O-foliamenthoyl-rutin (6) promoted HaCaT cell migration over scratch wounds, with compound 4 being the most effective. Our findings provide experimental data supporting the potential of quercetin-3-O-vicianoside (4) as a wound-healing agent.
Leaf Extracts of Invasive Woody Species Demonstrate Allelopathic Effects on the Growth of a Lawn Grass Mixture
Authors: Shelepova Olga V. , Tkacheva Ekaterina V. , Ivanovskii Aleksandr A. , Ozerova Ludmila V. , Vinogradova Yulia K.
Published: December 2023

Biochemical composition was studied in the leaf litter of alien woody species included in the 100 most aggressive invasive species of Europe: Ailanthus altissima, Quercus rubra, Acer negundo, Robinia pseudoacacia, and Elaeagnus angustifolia. Using GC-MS, we detected 187 metabolites in the leaf litter, which are phenolic acids and their derivatives, carbohydrates and their derivatives, polyphenolic compounds, cyclic esters, glycosides, and amino acids and their derivatives. Species-specific metabolites were identified for each species. The main allelochemicals in the leaf litter extract of Q. rubra are determined mainly by the relative abundance of phenolic and fatty acids and their esters, whereas those in the leaf litter extract of R. pseudoacacia are determined by carbohydrates and their derivatives and ester of fatty acid, and those in the leaf litter extract of A. altissima are determined by glycosides. Profiles of macro- and microelements were characterized. It was found that aqueous extracts of leaf litter from all the invasive woody plants under study have a negative effect on the seed germination and initial growth of Vicia cracca and Avena strigosa used for the reclamation of disturbed urban and industrial lands. At the same time, V. cracca is potentially more sensitive.
Can the Blended Application of Controlled-Release and Common Urea Effectively Replace the Common Urea in a Wheat–Maize Rotation System? A Case Study Based on a Long–Term Experiment
Authors: Zhang Ling , Xue Wen-Tao , Sun Hao , Hu Yun-Cai , Wu Rong , Tian Ye , Chen Yi-Shan , Ma Liang , Chen Qian , Du Ying , Bai Yang , Liu Shan-Jiang , Zou Guo-Yuan
Published: December 2023

The one-time application of blended urea (BU), combining controlled-release urea (CRU) and uncoated urea, has proven to be a promising nitrogen (N) management strategy. However, the long-term sustainability of blending urea remains largely unexplored. To assess whether a single application of blended urea could effectively replace split uncoated urea applications, a long-term field experiment was conducted in the North China Plain (NCP). The results indicated that, when compared to common urea (CU) at the optimal N rate (180 kg N ha−1), BU achieved comparable grain yields, N uptake and NUE (61% vs. 62). BU exhibited a 12% higher 0–20 cm soil organic nitrogen stock and a 9% higher soil organic carbon (C) stock. Additionally, BU reduced life–cycle reactive N (Nr) losses and the N footprint by 10%, and lowered greenhouse gas (GHG) emissions and the C footprint by 7%. From an economic analysis perspective, BU demonstrated comparable private profitability and a 3% greater ecosystem economic benefit. Therefore, BU under the optimal N rate has the potential to substitute split applications of common urea in the long–term and can be regarded as a sustainable N management strategy for wheat and maize production in the NCP.
Early Detection of Botrytis cinerea Infection in Cut Roses Using Thermal Imaging
Authors: Ha Suong Tuyet Thi , Kim Yong-Tae , In Byung-Chun
Published: December 2023

Botrytis cinerea (B. cinerea) causes gray mold disease (GMD), which results in physiological disorders in plants that decrease the longevity and economic value of horticultural crops. To prevent the spread of GMD during distribution, a rapid, early detection technique is necessary. Thermal imaging has been used for GMD detection in various plants, including potted roses; however, its application to cut roses, which have a high global demand, has not been established. In this study, we investigated the utility of thermal imaging for the early detection of B. cinerea infection in cut roses by monitoring changes in petal temperature after fungal inoculation. We examined the effects of GMD on the postharvest quality and petal temperature of cut roses treated with different concentrations of fungal conidial suspensions and chemicals. B. cinerea infection decreased the flower opening, disrupted the water balance, and decreased the vase life of cut roses. Additionally, the average temperature of rose petals was higher for infected flowers than for non-inoculated flowers. One day before the appearance of necrotic symptoms (day 1 of the vase period), the petal temperature in infected flowers was significantly higher, by 1.1 °C, than that of non-inoculated flowers. The GMD-induced increase in petal temperature was associated with the mRNA levels of genes related to ethylene, reactive oxygen species, and water transport. Furthermore, the increase in temperature caused by GMD was strongly correlated with symptom severity and fungal biomass. A multiple regression analysis revealed that the disease incidence in the petals was positively related to the petal temperature one day before the appearance of necrotic symptoms. These results show that thermography is an effective technique for evaluating changes in petal temperature and a possible method for early GMD detection in the cut flower industry.
A Possible Perspective of Recultivation with Arbuscular Mycorrhiza-Inoculated Drought-Tolerant Herbaceous Plants
Authors: Papp László , Habtemariam Akale Assamere , Brandt Sára , Cseh Péter , Heller Ádám , Péter Balázs , Szakály Ágnes Pappné , Kiszel Péter , Codogno Borbála , Bratek Zoltán , Tóth Zoltán
Published: December 2023

Using native species for urban green space is rather important nowadays. Plant cover on soil is necessary for agronomical and architectural investments as well as conservational programs, which all need minimal maintenance and have to be cost efficient. Commercially available seed mixtures for grasslands and lawns include species that partly originated from other mesoclimatic zones, and thus they may not be able to survive in the long-term, nor will they be adventive to the local ecosystem. With a focus on climate change, the most arid part of the Pannon geographical region was selected (near Törökszentmiklós in Nagykunság, Hungarian Great Plain). The local flora has adapted effectively to the environment; therefore, many species growing there were candidates for this study. Annuals and herbaceous perennials were investigated with respect to harvestability, reproducibility, decorativity, seed production, seed morphological characters (size, mass) and germination features. The selected 20 taxa were inoculated with INOQ Agri mycorrhiza (Rhizophagus irregularis) to increase the drought tolerance and biomass of the plants. Mycorrhizal frequency was significantly different among the taxa, reflecting various responses to the symbiotic interaction and possibly various mycorrhizal dependence of the plant species examined. We did not observe significantly higher colonization rate in most cases of the samples with artificial inoculation treatment. We conclude that the degraded mowed lawn soil that we used could contain propagules of AM fungi in a sufficient amount, so in the artificial grassland restorations, the additional AM inoculation treatment is not necessary to achieve a higher AM colonization rate.