bluuebunny/crossref_metadata_2025 huggingface dataset:
Porphyromonas gingivalis Virulence Factors and Clinical Significance in Periodontal Disease and Coronary Artery Diseases
Authors: Aleksijević Lorena Horvat , Aleksijević Marko , Škrlec Ivana , Šram Marko , Šram Miroslav , Talapko Jasminka
Published: October 2022

Porphyromonas gingivalis is a gram-negative, anaerobic bacterium that lives in the oral cavity. It is an integral part of the oral microbiome, which includes more than 500 types of bacteria. Under certain circumstances, as a consequence of virulence factors, it can become very destructive and proliferate to many cells in periodontal lesions. It is one of the causative agents present extremely often in dental plaque and is the main etiological factor in the development of periodontal disease. During various therapeutic procedures, P. gingivalis can enter the blood and disseminate through it to distant organs. This primarily refers to the influence of periodontal agents on the development of subacute endocarditis and can facilitate the development of coronary heart disease, atherosclerosis, and ischemic infarction. The action of P. gingivalis is facilitated by numerous factors of virulence and pathogenicity such as fimbriae, hemolysin, hemagglutinin, capsules, outer membrane vesicles, lipopolysaccharides, and gingipains. A special problem is the possibility of biofilm formation. P. gingivalis in a biofilm is 500 to 1000 times less sensitive to antimicrobial drugs than planktonic cells, which represents a significant problem in the treatment of infections caused by this pathogen.
Genetic Diversity of Coxiella burnetii in Iran by Multi-Spacer Sequence Typing
Authors: Mohabati Mobarez Ashraf , Baseri Neda , Khalili Mohammad , Mostafavi Ehsan , Stenos John , Esmaeili Saber
Published: October 2022

Coxiella burnetii, the zoonotic agent of Q fever, has a worldwide distribution including Iran. However, no information regarding the circulating genotype of this infection has been reported in Iran. This study aimed to evaluate the genetic diversity of C. burnetii in Iran using the multi-spacer sequence typing (MST) method. First, 14 positive C. burnetii samples (collected from four sheep, three goats, and seven cattle) were confirmed using quantitative polymerase chain reaction (qPCR) targeting the IS1111 gene. Then, ten spacers (Cox 2, 5, 18, 20, 22, 37, 51, 56, 57, and 61) were amplified using PCR for future MST analysis. The in-silico MST genotyping analysis of domestic ruminant samples revealed two new alleles (Cox5.11 and Cox56.15) in Cox5 and Cox56 loci that led to the emergence of four novel MST genotypes (MST62, 63, 64, and 65) and one MST genotype that has been previously described (MST61). This study showed the circulation of five MST C. burnetii genotypes among Iranian domestic ruminants. Understanding the C. burnetii genotypic profiles is critical in determining and preventing Q fever outbreaks.
Simultaneous Determination of Glibenclamide and Silymarin Released from Chitosan Microparticles by HPLC-ESI-MS Technique: Method Development and Validation
Authors: Condurache Mihaela-Iustina , Petrovici Anca-Roxana , Simionescu Natalia , Profire Bianca-Stefania , Confederat Luminita-Georgeta , Bujor Alexandra , Miron Anca , Profire Lenuta
Published: October 2022

The study aim was to develop and validate a high-performance liquid chromatography–electrospray ionization mass spectrometry (HPLC-ESI-MS) method to simultaneously determine glibenclamide (Gli) and silymarin (Sil) released from chitosan (CS) microparticles in aqueous solutions. The CS microparticles were synthesized using an ionic gelation method, and their morphology, swelling degree, encapsulation efficiency and active substance release were investigated. Gli and Sil were loaded in different concentrations, and their identification and quantification were performed using the HPLC-ESI-MS method, which was further validated. The drugs’ characteristic m/z was found in the higher intensity of retention time (Rt) (Gli, 8.909 min; Sil A, 5.41 min; and Sil B, 5.66 min). The method selectivity and precision are very good, and the blank solution proved no interference. The linearity of the answer function is very good for Sil A (R2 = 1), Sil B (R2 = 0.9998) and Gli (R2 = 0.9991). For Gli, we obtained a limit of detection (LOD) = 0.038 mg/mL and limit of quantification (LOQ) = 1.275 mg/mL; for Sil A, a LOD = 0.285 mg/mL and LOQ = 0.95 mg/mL; and for Sil B, a LOD = 0.045 mg/mL and LOQ = 0.15 mg/mL. A high-resolution HPLC-ESI-MS method was developed and validated, which allowed the simultaneous determination of Gli and Sil loaded in CS microparticles, in a concentration range of 0.025–1 mg/mL.
The Anticancer Effect of a Flavonoid-Rich Extract of Bergamot Juice in THP-1 Cells Engages the SIRT2/AKT/p53 Pathway
Authors: Maugeri Alessandro , Russo Caterina , Musumeci Laura , Lombardo Giovanni Enrico , De Sarro Giovambattista , Barreca Davide , Cirmi Santa , Navarra Michele
Published: October 2022

Novel targets are constantly sought to fight hematologic malignancies. In this regard, high levels of SIRT2 expression are associated with unfavorable prognosis of acute myeloid leukemia. The interest in the plant kingdom has allowed the identification of ever-new anti-leukemic agents. Citrus × bergamia (bergamot) was proved to possess anticancer properties, yet no evidence is available regarding leukemia. For the first time, we studied the potential anti-leukemic effect of a flavonoid-rich extract of bergamot juice (BJe) in THP-1 cells, investigating the underlying mechanisms. Our findings showed that BJe reduced THP-1 cell proliferation, without affecting that of primary PBMCs, blocking the cell cycle in S phase and inducing apoptosis. Triggering of both extrinsic and intrinsic apoptotic pathways was witnessed by cleavage of caspase-8 and -9, which in turn activated caspase-3 and PARP. Interestingly, the increased p53 acetylation in THP-1 cells underlies SIRT2 inhibition by BJe, that was proved also in the isolated enzyme. Moreover, BJe hampered SIRT2 also by lowering its gene expression. Finally, BJe reduced AKT phosphorylation, which we hypothesized being the joining link between SIRT2 and p53, that play a pivotal role in BJe-induced cell cycle arrest and apoptosis in THP-1 cells. Our results suggest BJe as a potential anti-leukemic agent, via targeting of the SIRT2/AKT/p53 pathway.
Chromosome Numbers and Genome Sizes of All 36 Duckweed Species (Lemnaceae)
Authors: Hoang Phuong T. N. , Fuchs Jörg , Schubert Veit , Tran Tram B. N. , Schubert Ingo
Published: October 2022

Usually, chromosome sets (karyotypes) and genome sizes are rather stable for distinct species and therefore of diagnostic value for taxonomy. In combination with (cyto)genomics, both features provide essential cues for genome evolution and phylogenetic relationship studies within and between taxa above the species level. We present for the first time a survey on chromosome counts and genome size measurement for one or more accessions from all 36 duckweed species and discuss the evolutionary impact and peculiarities of both parameters in duckweeds.
Bioassay-Guided Skin-Beneficial Effects of Fractionated Sideritis raeseri subsp. raeseri Extract
Authors: Krgović Nemanja , Jovanović Miloš , Aradski Ana Alimpić , Janković Teodora , Stević Tatjana , Zdunić Gordana , Laušević Sonja Duletić , Šavikin Katarina
Published: October 2022

This study aimed to evaluate the potentials for skin-beneficial effects of the hydroethanolic extract of the aerial parts of ironwort (Sideritis raeseri Boiss. & Heldr. subsp. raeseri) and its fractions (petroleum ether, ethyl acetate, n-butanol and water). For these purposes, chemical analysis and in vitro antioxidant, anti-tyrosinase and antimicrobial assays, as well as determination of sun protection factor (SPF), were performed. The highest total phenolic content and quantity of individual flavonoids, chlorogenic acid and verbascoside were determined in the n-butanol fraction, which is in line with results obtained for antioxidant activity. The greatest antimicrobial activity against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa, the most common causative agents of the skin infections in humans, was exhibited by the ethyl acetate fraction. The strongest anti-tyrosinase activity was shown by the hydroethanolic extract (52.64% of inhibition at 100 μg/mL). Almost all tested samples showed photoprotective activity with SPF higher than 6 obtained at a low concentration of 0.1 mg/mL, which was more than 15 for the butanol fraction. These findings revealed that the hydroethanolic extract of S. raeseri aerial parts could be a valuable ingredient in the formulation of cosmetic and medicinal products intended to overcome various skin disorders.
Ultrasound-Assisted Natural Deep Eutectic Solvents Extraction of Bilberry Anthocyanins: Optimization, Bioactivities, and Storage Stability
Authors: Jovanović Miloš S. , Krgović Nemanja , Živković Jelena , Stević Tatjana , Zdunić Gordana , Bigović Dubravka , Šavikin Katarina
Published: October 2022

Bilberry fruits (Vaccinium myrtillus L.) are one of the richest natural sources of anthocyanins and are widely used due to their pharmacological and nutritional properties. To ensure their maximum application potential, it is necessary to overcome the limitations of conventional extraction solvents and techniques. This study aimed to develop a green method for bilberry anthocyanin extraction using natural deep eutectic solvents (NaDES) integrated with ultrasound-assisted extraction (UAE) in order to define extraction conditions that will prevent decomposition of the anthocyanins or the loss of bioactivity. After a screening of ten different NaDES, choline chloride:sorbitol (1:1) was selected as the most effective. Furthermore, the influence analysis and optimization of the NaDES–UAE extraction conditions were carried out employing response surface methodology. The optimal conditions were found to be an extraction time of 37.63 min, a temperature of 48.38 °C, and 34.79% (w/w) water in NaDES. The extraction yields of target compounds under optimized extraction conditions were 0.27 mg/g DW of cyanidin-3-O-glucoside and 2.12 mg CGE/g DW of TAC. The obtained optimized extract showed promising radical scavenging and antimicrobial activity. A stability study with the optimized extract revealed that refrigerated storage at 4 °C in the dark provided the best anthocyanins preservation. Overall, the developed NaDES-UAE method showed promising application potential and can be considered as a high-efficiency green alternative to conventional anthocyanins extraction methods, enabling the preservation of active ingredients and the bioactivity of extracts.
Understanding the Influence of Socioeconomic Variables on Medicinal Plant Knowledge in the Peruvian Andes
Authors: Corroto Fernando , Gamarra Torres Oscar Andrés , Macía Manuel J.
Published: October 2022

In this study, we analyze the impact of 18 socioeconomic factors at individual, family, and locality levels to understand their influence on medicinal plant knowledge (MPK) in four provinces and 12 localities of the northern Andes of Peru. We interviewed 50 participants per locality (totaling 600 people) from lowlands and highlands ecoregions. The participants were balanced in terms of generations and gender. We performed multivariate statistical analyses—generalized linear mixed models (GLMMs) and nonmetric multidimensional scaling (NMDS) ordinations—that showed the influence of each socioeconomic variable on the medicinal plant knowledge of people in the different sites. At the individual level, we found that most participants with higher MPK were women, elders, people with lower levels of education and job qualifications, non-migrants, and participants who have lived for a long period in the same region. At the family level, we found that participants living in low economic conditions with few material goods, including their means of transport, tools possession, access to technology, farm size, number of farm animals, and house quality, had higher MPK. At the locality level, we found that people living in more isolated areas with scarce regional services, such as access to paved roads, hospitals, big markets, tourist development, and chlorination of drinking water, had higher MPK. In short, people with less access to modern services and with low economic resources are the main depositaries of MPK. Policy makers and decision makers should consider the significance of MPK in alleviating health problems and diseases in Andean regions, especially for people with rural livelihoods. This local botanical knowledge of medicinal plants should be preserved in the area as a great natural heritage for humanity.
Chitosan Oligosaccharide Prevents Afatinib-Induced Barrier Disruption and Chloride Secretion through Modulation of AMPK, PI3K/AKT, and ERK Signaling in T84 Cells
Authors: Mehmood Tahir , Pichyangkura Rath , Muanprasat Chatchai
Published: October 2022

Diarrhea is an important adverse effect of epidermal growth factor receptor-tyrosine kinase inhibitors, especially afatinib. Novel antidiarrheal agents are needed to reduce epidermal growth factor receptor-tyrosine kinase inhibitor-associated diarrhea to improve the quality of life and treatment outcome in cancer patients. This study aimed to investigate the anti-diarrheal activity of chitosan oligosaccharide against afatinib-induced barrier disruption and chloride secretion in human intestinal epithelial cells (T84 cells). Chitosan oligosaccharide (100 μg/mL) prevented afatinib-induced barrier disruption determined by changes in transepithelial electrical resistance and FITC-dextran flux in the T84 cell monolayers. In addition, chitosan oligosaccharide prevented afatinib-induced potentiation of cAMP-induced chloride secretion measured by short-circuit current analyses in the T84 cell monolayers. Chitosan oligosaccharide induced the activation of AMPK, a positive regulator of epithelial tight junction and a negative regulator of cAMP-induced chloride secretion. Moreover, chitosan oligosaccharide partially reversed afatinib-induced AKT inhibition without affecting afatinib-induced ERK inhibition via AMPK-independent mechanisms. Collectively, this study reveals that chitosan oligosaccharide prevents the afatinib-induced diarrheal activities in T84 cells via both AMPK-dependent and AMPK-independent mechanisms. Chitosan oligosaccharide represents a promising natural polymer-derived compound for further development of treatment for afatinib-associated diarrheas.
The Green Era of Food Packaging: General Considerations and New Trends
Authors: Maurizzi Enrico , Bigi Francesco , Quartieri Andrea , De Leo Riccardo , Volpelli Luisa Antonella , Pulvirenti Andrea
Published: October 2022

Recently, academic research and industries have gained awareness about the economic, environmental, and social impacts of conventional plastic packaging and its disposal. This consciousness has oriented efforts towards more sustainable materials such as biopolymers, paving the way for the “green era” of food packaging. This review provides a schematic overview about polymers and blends of them, which are emerging as promising alternatives to conventional plastics. Focus was dedicated to biopolymers from renewable sources and their applications to produce sustainable, active packaging with antimicrobial and antioxidant properties. In particular, the incorporation of plant extracts, food-waste derivatives, and nano-sized materials to produce bio-based active packaging with enhanced technical performances was investigated. According to recent studies, bio-based active packaging enriched with natural-based compounds has the potential to replace petroleum-derived materials. Based on molecular composition, the natural compounds can diversely interact with the native structure of the packaging materials, modulating their barriers, optical and mechanical performances, and conferring them antioxidant and antimicrobial properties. Overall, the recent academic findings could lead to a breakthrough in the field of food packaging, opening the gates to a new generation of packaging solutions which will be sustainable, customised, and green.
Investigations on the Fatigue Behaviour of 3D-Printed Continuous Carbon Fibre-Reinforced Polymer Tension Straps
Authors: Vidrih Tadej , Winiger Peter , Triantafyllidis Zafiris , Ott Valentin , Terrasi Giovanni P.
Published: October 2022

The focus of this research is an investigation on the fatigue behaviour of unidirectional 3D-printed continuous carbon fibre-reinforced polymer (CFRP) tension straps with a polyamide matrix (PA12). Conventionally produced tension straps are becoming established components in the mechanical as well as the civil engineering sector, e.g., as rigging systems for sailing boats and cranes and—recently introduced—as network arch bridge hangers. All these structures are subjected to high fatigue loads, and although it is commonly reported that carbon fibre-reinforced polymers show excellent fatigue resistance, there is limited understanding of the behaviour of CFRP loop elements under such loads, especially in combination with fretting at the attachment points. Research on this topic was performed at Empa in the past decade on thermoset CFRP straps, but never before with 3D-printed continuous CFRP straps with a thermoplastic matrix. This paper examines an additive manufacturing and post-consolidation method for producing the straps and presents initial results on their fatigue performance, which show that the fatigue endurance limit of the investigated 3D-printed and post-consolidated CFRP strap design is acceptable, when compared to steel tendons. However, it is still 20% lower than conventionally produced CFRP straps using out-of-autoclave unidirectional carbon fibre prepregs. The reasons for these findings and potential future improvements are discussed.
Kinetic and Isothermal Investigations on the Use of Low Cost Coconut Fiber-Polyaniline Composites for the Removal of Chromium from Wastewater
Authors: Jha Stuti , Gaur Rama , Shahabuddin Syed , Ahmad Irfan , Sridewi Nanthini
Published: October 2022

Pollution due to various heavy metals is increasing at an alarming rate. Removal of hexavalent chromium from the environment is a significant and challenging issue due to its toxic effects on the ecosystem. Development of a low-cost adsorbent with better adsorption efficiency is presently required. In this study, waste coconut fibers (CF) were used to prepare its composite with polyaniline (PANI) via in-situ oxidation. The obtained composites with varying loading of PANI (15, 25, 50, and 75% w/w) were characterized by FE-SEM, TGA, and FTIR spectroscopy. The prepared composites were evaluated for their adsorption performance for removal of Cr(VI). It was concluded that the composite with 50% w/w polyaniline loading on coconut fiber exhibited a maximum adsorption efficiency of 93.11% in 30 min. The effect of pH, dosage, and concentration of the aqueous solution of chromium on the Cr(VI) adsorption efficiency of the composite was also studied. From the optimization studies it was observed that the absorbents exhibited the best adsorption response for Cr(VI) removal with 0.25 mg/mL adsorbent at pH 4, in 30 min. The effect of pH, dosage, and concentration of the aqueous solution of chromium on the Cr(VI) adsorption efficiency of the composite was also studied. This study highlights the application of low-cost adsorbent as a potential candidate for the removal of hexavalent chromium. A detailed study on the adsorption kinetics and isothermal analysis was conducted for the removal of Cr(VI) from aqueous solution using coconut fiber-polyaniline composite. From the kinetic investigation, the adsorption was found to follow the pseudo second order model. The data obtained were best fitted to the Elovich model confirming the chemisorption of the Cr(VI) on coconut polymer composites. The analysis of the isothermal models indicated monolayer adsorption based on the Langmuir adsorption model.
A Brief Review on Advanced Sandwich Structures with Customized Design Core and Composite Face Sheet
Authors: Sahu Santosh Kumar , Sreekanth P. S. Rama , Reddy S. V. Kota
Published: October 2022

Sandwich structures are a class of multifunctional high-performance structural composites that have the advantages of being lightweight, of a high strength-to-weight ratio, and of high specific energy absorption capabilities. The creative design of the core along with the apposite material selection for the fabrication of the face sheet and core are the two prerequisites with encouraging areas for further expedition towards the fabrication of advanced composite sandwich structures. The current review work focused on different types of core designs, such as truss, foam, corrugated, honeycomb, derivative, hybrid, hollow, hierarchical, gradient, folded, and smart core along with different composite materials accessible for face sheet fabrication, including fiber-reinforced composite, metal matrix composite, and polymer matrix composite are considered. The joining method plays a major role for the performance evolution of sandwich structures, which were also investigated. Further discussions are aligned to address major challenges in the fabrication of sandwich structures and further enlighten the future direction of the advanced composite sandwich structure. Finally, the work is summarized with a brief conclusion. This review article provides wider guidelines for researchers in designing and manufacturing next-generation lightweight multilayer core sandwich structures.
One-Shot Synthesis of Thermoplastic Polyurethane Based on Bio-Polyol (Polytrimethylene Ether Glycol) and Characterization of Micro-Phase Separation
Authors: Jung Yang-Sook , Lee Sunhee , Park Jaehyeung , Shin Eun-Joo
Published: October 2022

In this study, a series of bio-based thermoplastic polyurethane (TPU) was synthesized via the solvent-free one-shot method using 100% bio-based polyether polyol, prepared from fermented corn, and 1,4-butanediol (BDO) as a chain extender. The average molecular weight, degree of phase separation, thermal and mechanical properties of the TPU-based aromatic (4,4-methylene diphenyl diisocyanate: MDI), and aliphatic (bis(4-isocyanatocyclohexyl) methane: H12MDI) isocyanates were investigated by gel permeation chromatography, Fourier transform infrared spectroscopy, atomic force microscopy, X-ray Diffraction, differential scanning calorimetry, dynamic mechanical thermal analysis, and thermogravimetric analysis. Four types of micro-phase separation forms of a hard segment (HS) and soft segment (SS) were suggested according to the [NCO]/[OH] molar ratio and isocyanate type. The results showed (a) phase-mixed disassociated structure between HS and SS, (b) hydrogen-bonded structure of phase-separated between HS and SS forming one-sided hard domains, (c) hydrogen-bonded structure of phase-mixed between HS, and SS and (d) hydrogen-bonded structure of phase-separated between HS and SS forming dispersed hard domains. These phase micro-structure models could be matched with each bio-based TPU sample. Accordingly, H-BDO-2.0, M-BDO-2.0, H-BDO-2.5, and M-BDO-3.0 could be related to the (a)—form, (b)—form, (c)—form, and (d)—form, respectively.
Recent Advances in Functionalization of Cotton Fabrics with Nanotechnology
Authors: Abou Elmaaty Tarek M. , Elsisi Hanan , Elsayad Ghada , Elhadad Hagar , Plutino Maria Rosaria
Published: October 2022

Nowadays, consumers understand that upgrading their traditional clothing can improve their lives. In a garment fabric, comfort and functional properties are the most important features that a wearer looks for. A variety of textile technologies are being developed to meet the needs of customers. In recent years, nanotechnology has become one of the most important areas of research. Nanotechnology’s unique and useful characteristics have led to its rapid expansion in the textile industry. In the production of high-performance textiles, various finishing, coating, and manufacturing techniques are used to produce fibers or fabrics with nano sized (10−9) particles. Humans have been utilizing cotton for thousands of years, and it accounts for around 34% of all fiber production worldwide. The clothing industry, home textile industry, and healthcare industry all use it extensively. Nanotechnology can enhance cotton fabrics’ properties, including antibacterial activity, self-cleaning, UV protection, etc. Research in the field of the functionalization of nanotechnology and their integration into cotton fabrics is presented in the present study.
How Religious Attitudes Are Associated with Depression in Turkish Society during the COVID-19 Pandemic: The Mediating Role of Meaning in Life
Authors: Koçak Orhan
Published: October 2022

Religion has an important role in coping with the social and psychological problems encountered in human life. However, one topic has not been studied enough, namely that religious attitudes, which are adopting and living religious values, have positive contributions by changing the characteristics of individuals coping with problems. In this study, the indirect role of meaning in life in the association of religion with depression was examined. The current study was conducted online and was cross-sectional and quantitative, with 1571 individuals aged 18–30 in Turkey. For this purpose, scales of religious attitude, depression, and meaning in life were used. First confirmatory factor analysis, and then correlation and multiple regression analyses, were carried out to test the hypotheses using the SPSS, Amos, and Process Macro Plug-in programs. According to the test results, religious attitude has positive relations with meaning in life, and meaning in life has a negative association with depression. Therefore, it was understood that the presence of meaning in life and the search for meaning in life had mediating roles in the relations of religious attitudes with depression.
Trade and the Mosque: An Investigation of Commercial Activities and Mosques in Antalya with Spatial, Legal and Functional Recommendations
Authors: Kepenek Büşra Selin , Kepenek Engin , Bakır İbrahim
Published: October 2022

Expenditures, such as repair, heating and lighting costs, and payment of mosque staff’s salaries, are the main cost items that need to be satisfied for mosques to continue their services. Throughout history, these expenses have been met sometimes with the state budget, sometimes through waqfs and associations, and sometimes with the cash aid provided by the people directly in Turkey. Adding a commercial function to the mosque, which was built for the sake of continuous income, has been seen in the history of Turkey since the Ottoman period. However, the commercial units dedicated to the mosque during this period were not always close to the mosque; sometimes they could also be located in very remote location. Today, a type of structure is built which can be called a trade and religious building in the lower floor of the mosque, or as a part of the same floor due to space shortage, cost reduction etc. This type of building, which was interpreted with different perspectives, caused problems in terms of perceptibility, ratio and proportion, visuality, accessibility, noise pollution, and the moral values of the mosque, and even became the subject of lawsuits for the closure of commercial functions. In this context, the commercial functions that the mosques added to the structure in order to find financial resources were examined throughout the city center of Antalya. Accordingly, the architectural projects and zoning status of the 15 identified mosques were examined, and a spatial and configuration analysis was made. The functionality of the mosques was discussed with the mosque community, its officials, and the people in the area close to the mosque via focus group discussions, and examples of the issue brought to the judiciary were examined.
Soft Robots’ Dynamic Posture Perception Using Kirigami-Inspired Flexible Sensors with Porous Structures and Long Short-Term Memory (LSTM) Neural Networks
Authors: Shu Jing , Wang Junming , Lau Sanders Cheuk Yin , Su Yujie , Heung Kelvin Ho Lam , Shi Xiangqian , Li Zheng , Tong Raymond Kai-yu
Published: October 2022

Soft robots can create complicated structures and functions for rehabilitation. The posture perception of soft actuators is critical for performing closed-loop control for a precise location. It is essential to have a sensor with both soft and flexible characteristics that does not affect the movement of a soft actuator. This paper presents a novel end-to-end posture perception method that employs flexible sensors with kirigami-inspired structures and long short-term memory (LSTM) neural networks. The sensors were developed with conductive sponge materials. With one-step calibration from the sensor output, the posture of the soft actuator could be calculated by the LSTM network. The method was validated by attaching the developed sensors to a soft fiber-reinforced bending actuator. The results showed the accuracy of posture prediction of sponge sensors with three kirigami-inspired structures ranged from 0.91 to 0.97 in terms of R2. The sponge sensors only generated a resistive torque value of 0.96 mNm at the maximum bending position when attached to a soft actuator, which would minimize the effect on actuator movement. The kirigami-inspired flexible sponge sensor could in future enhance soft robotic development.
Assessment of the Performance of a Portable, Low-Cost and Open-Source Device for Luminance Mapping through a DIY Approach for Massive Application from a Human-Centred Perspective
Authors: Salamone Francesco , Sibilio Sergio , Masullo Massimiliano
Published: October 2022

Ubiquitous computing has enabled the proliferation of low-cost solutions for capturing information about the user’s environment or biometric parameters. In this sense, the do-it-yourself (DIY) approach to build new low-cost systems or verify the correspondence of low-cost systems compared to professional devices allows the spread of application possibilities. Following this trend, the authors aim to present a complete DIY and replicable procedure to evaluate the performance of a low-cost video luminance meter consisting of a Raspberry Pi and a camera module. The method initially consists of designing and developing a LED panel and a light cube that serves as reference illuminance sources. The luminance distribution along the two reference light sources is determined using a Konica Minolta luminance meter. With this approach, it is possible to identify an area for each light source with an almost equal luminance value. By applying a frame that covers part of the panel and shows only the area with nearly homogeneous luminance values and applying the two systems in a dark space in front of the low-cost video luminance meter mounted on a professional reference camera photometer LMK mobile air, it is possible to check the discrepancy in luminance values between the low-cost and professional systems when pointing different homogeneous light sources. In doing so, we primarily consider the peripheral shading effect, better known as the vignetting effect. We then differentiate the correction factor S of the Radiance Pcomb function to better match the luminance values of the low-cost system to the professional device. We also introduce an algorithm to differentiate the S factor depending on the light source. In general, the DIY calibration process described in the paper is time-consuming. However, the subsequent applications in various real-life scenarios allow us to verify the satisfactory performance of the low-cost system in terms of luminance mapping and glare evaluation compared to a professional device.
Adaptive Multi-ROI Agricultural Robot Navigation Line Extraction Based on Image Semantic Segmentation
Authors: Li Xia , Su Junhao , Yue Zhenchao , Duan Fangtao
Published: October 2022

Automated robots are an important part of realizing sustainable food production in smart agriculture. Agricultural robots require a powerful and precise navigation system to be able to perform tasks in the field. Aiming at the problems of complex image background, as well as weed and light interference factors of the visual navigation system in field and greenhouse environments, a Faster-U-net model that retains the advantages of the U-net model feature jump connection is proposed. Based on the U-net model, pruning and optimization were carried out to predict crop ridges. Firstly, a corn dataset was trained to obtain the weight of the corn dataset. Then, the training weight of the obtained corn dataset was used as the pretraining weight for the cucumber, wheat, and tomato datasets, respectively. The three datasets were trained separately. Finally, the navigation line between ridges and the yaw angle of the robot were generated by B-spline curve fitting. The experimental results showed that the parameters of the improved path segmentation model were reduced by 65.86%, and the mPA was 97.39%. The recognition accuracy MIoU of the Faster-U-net model for maize, tomatoes, cucumbers, and wheat was 93.86%, 94.01%, 93.14%, and 89.10%, respectively. The processing speed of the single-core CPU was 22.32 fps/s. The proposed method had strong robustness in predicting rows of different crops. The average angle difference of the navigation line under a ridge environment such as that for corn, tomatoes, cucumbers, or wheat was 0.624°, 0.556°, 0.526°, and 0.999°, respectively. This research can provide technical support and reference for the research and development of intelligent agricultural robot navigation equipment in the field.
Microsoft HoloLens 2 in Medical and Healthcare Context: State of the Art and Future Prospects
Authors: Palumbo Arrigo
Published: October 2022

In the world reference context, although virtual reality, augmented reality and mixed reality have been emerging methodologies for several years, only today technological and scientific advances have made them suitable to revolutionize clinical care and medical contexts through the provision of enhanced functionalities and improved health services. This systematic review provides the state-of-the-art applications of the Microsoft® HoloLens 2 in a medical and healthcare context. Focusing on the potential that this technology has in providing digitally supported clinical care, also but not only in relation to the COVID-19 pandemic, studies that proved the applicability and feasibility of HoloLens 2 in a medical and healthcare scenario were considered. The review presents a thorough examination of the different studies conducted since 2019, focusing on HoloLens 2 medical sub-field applications, device functionalities provided to users, software/platform/framework used, as well as the study validation. The results provided in this paper could highlight the potential and limitations of the HoloLens 2-based innovative solutions and bring focus to emerging research topics, such as telemedicine, remote control and motor rehabilitation.
Health-zkIDM: A Healthcare Identity System Based on Fabric Blockchain and Zero-Knowledge Proof
Authors: Bai Tianyu , Hu Yangsheng , He Jianfeng , Fan Hongbo , An Zhenzhou
Published: October 2022

The issue of identity authentication for online medical services has been one of the key focuses of the healthcare industry in recent years. Most healthcare organizations use centralized identity management systems (IDMs), which not only limit the interoperability of patient identities between institutions of healthcare, but also create isolation between data islands. The more important matter is that centralized IDMs may lead to privacy disclosure. Therefore, we propose Health-zkIDM, a decentralized identity authentication system based on zero-knowledge proof and blockchain technology, which allows patients to identify and verify their identities transparently and safely in different health fields and promotes the interaction between IDM providers and patients. The users in Health-zkIDM are uniquely identified by one ID registered. The zero-knowledge proof technology is deployed on the client, which provides the user with a proof of identity information and automatically verifies the user’s identity after registration. We implemented chaincodes on the Fabric, including the upload of proof of identity information, identification, and verification functions. The experiences show that the performance of the Health-zkIDM system can achieve throughputs higher than 400 TPS in Caliper.
Deep-E Enhanced Photoacoustic Tomography Using Three-Dimensional Reconstruction for High-Quality Vascular Imaging
Authors: Zheng Wenhan , Zhang Huijuan , Huang Chuqin , McQuillan Kaylin , Li Huining , Xu Wenyao , Xia Jun
Published: October 2022

Linear-array-based photoacoustic computed tomography (PACT) has been widely used in vascular imaging due to its low cost and high compatibility with current ultrasound systems. However, linear-array transducers have inherent limitations for three-dimensional imaging due to the poor elevation resolution. In this study, we introduced a deep learning-assisted data process algorithm to enhance the image quality in linear-array-based PACT. Compared to our earlier study where training was performed on 2D reconstructed data, here, we utilized 2D and 3D reconstructed data to train the two networks separately. We then fused the image data from both 2D and 3D training to get features from both algorithms. The numerical and in vivo validations indicate that our approach can improve elevation resolution, recover the true size of the object, and enhance deep vessels. Our deep learning-assisted approach can be applied to translational imaging applications that require detailed visualization of vascular features.
Development and Validation of HPLC-DAD Method for the Determination of Favipiravir and Studying the Impact of Vitamin C on the Pharmacokinetics of COVID-19 Antiviral Drug Favipiravir
Authors: Hailat Mohammad , Al-Ani Israa , Zakareia Zainab , Al-Shdefat Ramadan , Al-Meanazel Osaid , Anwer Md. Khalid , Hamad Mohammed , Abu Rayyan Walid , Awad Riad , Abu Dayyih Wael
Published: October 2022

A novel, sensitive, and low-cost HPLC method for the rapid determination of favipiravir (FVR) in rat plasma was developed and validated, and the effect of vitamin C on FVR pharmacokinetic parameters was investigated. FVR and oxcarbazepine (IS) were separated using a mobile phase of 50% acetonitrile and 50% water (with 0.25% trifluoroacetic acid) at 1.0 mL/min flow rate and detected at λmax 289 nm. The intra- and interday values for FVR in plasma were less than 15%, with low, medium, and high QC levels for the relative recovery rate, according to ICH guidelines. Cmax values in the control and experimental groups were 558 ± 124.42 and 979.13 ± 138.10 ng/mL, respectively; t1/2 values were 7.15 ± 1.60 and 9.09 ± 1.14 h, AUC(0-t) values were 5697.70 ± 536.58 and 7381.62 ± 1577.58 ng.h/mL, and AUC(0-∞) values were 5697.70 ± 536.58 and 8192.36 ±  1721.67, respectively. According to the results, the experimental group’s Cmax of FVR was 75.17% higher than the control group’s, the Vz/F was lower, and the t1/2 was 1.86 h longer. The technique developed for determining FVR in plasma was useful for FVR pharmacokinetics and food–drug interaction investigations.
User Preferences towards Hyperloop Systems: Initial Insights from Germany
Authors: Abouelela Mohamed , Al Haddad Christelle , Islam Md Ashraful , Antoniou Constantinos
Published: October 2022

The rapidly evolving urbanization is generating unprecedented travel demand, notably in intercity travel. With increasing challenges in motorized traffic, innovative and sustainable transport modes are more than ever necessary. The Hyperloop system is an emerging transportation mode with the potential to change long–distance commutes, especially between cities. There is a need for, but also gap in, understanding this potentially emerging transport mode. This study aims at filling this gap by deploying a stated preference study in Germany, in which data was collected for 786 respondents with 5640 scenarios, to investigate the factors impacting users’ preferences towards Hyperloop systems. Models were developed to examine factors impacting the immediate Hyperloop adoption (in the first year of its implementation), but also the choice between Hyperloop and other long–distance travel modes, such as airplanes and high-speed trains. Results indicate that mode-related characteristics (travel time, travel cost, safety), individual characteristics (gender, income level, availability of a driving license, access to a car, familiarity with the Hyperloop system), the current satisfaction level with high-speed trains and airplanes, and personality traits (confidence, affinity to technology) are the most significant factors in the choice and early adoption of Hyperloop systems.
Calculation of Costs Related to Death by Suicide in Finland
Authors: Solin Pia , Tamminen Nina , Seppänen Anna , Partonen Timo
Published: October 2022

There are relatively few calculations of the costs related to suicides. The aim was to produce a monetary estimate of the costs incurred as a result of suicides. The costs were divided into three categories: (1) loss of labor input, (2) costs directly following a suicide, and (3) costs associated with family members. In our two sample cases, the costs related to suicides were from EUR 309,020 to EUR 456,279. By putting the monetary costs of suicide prevention intervention and the calculation of costs related to a death by suicide side by side, this study may help when allocating funding for effective intervention.
Role of Heparanase and Syndecan-1 in HSV-1 Release from Infected Cells
Authors: Sharma Pankaj , Kapoor Divya , Shukla Deepak
Published: October 2022

Herpes Simplex Virus 1 (HSV-1) is a neurotropic human virus that belongs to the Alphaherpesvirinae subfamily of Herpesviridae. Establishment of its productive infection and progression of disease pathologies depend largely on successful release of virions from the virus-producing cells. HSV-1 is known to exploit many host factors for its release. Recent studies have shown that heparanase (HPSE) is one such host enzyme that is recruited for this purpose. It is an endoglycosidase that cleaves heparan sulfate (HS) from the surface of infected cells. HS is a virus attachment coreceptor that is commonly found on cell surfaces as HS proteoglycans e.g., syndecan-1 (SDC-1). The current model suggests that HSV-1 during the late stage of infection upregulates HPSE, which in turn enhances viral release by removing the virus-trapping HS moieties. In addition to its role in directly enabling viral release, HPSE accelerates the shedding of HS-containing ectodomains of SDC-1, which enhances HSV-1 release via a similar mechanism by upregulating CREB3 and COPII proteins. This review outlines the role of HPSE and SDC-1 as newly assigned host factors that facilitate HSV-1 release during a lytic infection cycle.
Temperature Influences the Interaction between SARS-CoV-2 Spike from Omicron Subvariants and Human ACE2
Authors: Gong Shang Yu , Ding Shilei , Benlarbi Mehdi , Chen Yaozong , Vézina Dani , Marchitto Lorie , Beaudoin-Bussières Guillaume , Goyette Guillaume , Bourassa Catherine , Bo Yuxia , Medjahed Halima , Levade Inès , Pazgier Marzena , Côté Marceline , Richard Jonathan , Prévost Jérémie , Finzi Andrés
Published: October 2022

SARS-CoV-2 continues to infect millions of people worldwide. The subvariants arising from the variant-of-concern (VOC) Omicron include BA.1, BA.1.1, BA.2, BA.2.12.1, BA.4, and BA.5. All possess multiple mutations in their Spike glycoprotein, notably in its immunogenic receptor-binding domain (RBD), and present enhanced viral transmission. The highly mutated Spike glycoproteins from these subvariants present different degrees of resistance to recognition and cross-neutralisation by plasma from previously infected and/or vaccinated individuals. We have recently shown that the temperature affects the interaction between the Spike and its receptor, the angiotensin converting enzyme 2 (ACE2). The affinity of RBD for ACE2 is significantly increased at lower temperatures. However, whether this is also observed with the Spike of Omicron and sub-lineages is not known. Here we show that, similar to other variants, Spikes from Omicron sub-lineages bind better the ACE2 receptor at lower temperatures. Whether this translates into enhanced transmission during the fall and winter seasons remains to be determined.
The Spatial Diffusion of Cherry Leaf Roll Virus Revealed by a Bayesian Phylodynamic Analysis
Authors: Shen Jianguo , Guo Jing , Chen Xihong , Cai Wei , Du Zhenguo , Zhang Yongjiang
Published: October 2022

Cherry leaf roll virus (CLRV) is an important plant pathogen that causes severe and detrimental effects on cherry and other fruit plants. Despite recent progress in plant pathology, molecular biology, and population genetics of CLRV, the spatiotemporal spread of this virus remains poorly studied. In this study, we employed a Bayesian phylodynamics framework to investigate the spatial diffusion patterns of CLRV by analyzing the coat protein gene sequences of 81 viral isolates collected from five different countries. Consistent with the trade of cherry, our Bayesian phylodynamic analyses pointed to viral origins in New Zealand and identified multiple migration pathways between Germany and other countries, suggesting that Germany has played an important role in CLRV transmission. The results of our study will be useful in developing sustainable management strategies to control this pathogen.
Pin-Pointing the Key Hubs in the IFN-γ Pathway Responding to SARS-CoV-2 Infection
Authors: Toro Ayelen , Lage-Vickers Sofia , Bizzotto Juan , Vilicich Felipe , Sabater Agustina , Pascual Gaston , Ledesma-Bazan Sabrina , Sanchis Pablo , Ruiz Maria Sol , Arevalo Ana Paula , Porfido Jorge L. , Abbate Mercedes , Seniuk Rocio , Labanca Estefania , Anselmino Nicolas , Navone Nora M. , Alonso Daniel F. , Vazquez Elba , Crispo Martina , Cotignola Javier , Gueron Geraldine
Published: October 2022

Interferon gamma (IFN-γ) may be potential adjuvant immunotherapy for COVID-19 patients. In this work, we assessed gene expression profiles associated with the IFN-γ pathway in response to SARS-CoV-2 infection. Employing a case-control study from SARS-CoV-2-positive and -negative patients, we identified IFN-γ-associated pathways to be enriched in positive patients. Bioinformatics analyses showed upregulation of MAP2K6, CBL, RUNX3, STAT1, and JAK2 in COVID-19-positive vs. -negative patients. A positive correlation was observed between STAT1/JAK2, which varied alongside the patient’s viral load. Expression of MX1, MX2, ISG15, and OAS1 (four well-known IFN-stimulated genes (ISGs)) displayed upregulation in COVID-19-positive vs. -negative patients. Integrative analyses showcased higher levels of ISGs, which were associated with increased viral load and STAT1/JAK2 expression. Confirmation of ISGs up-regulation was performed in vitro using the A549 lung cell line treated with Poly (I:C), a synthetic analog of viral double-stranded RNA; and in different pulmonary human cell lines and ferret tracheal biopsies infected with SARS-CoV-2. A pre-clinical murine model of Coronavirus infection confirmed findings displaying increased ISGs in the liver and lungs from infected mice. Altogether, these results demonstrate the role of IFN-γ and ISGs in response to SARS-CoV-2 infection, highlighting alternative druggable targets that can boost the host response.