bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthetic MRI, multiplexed sensitivity encoding, and BI-RADS for benign and malignant breast cancer discrimination
Authors: Liu Jinrui , Xu Mengying , Ren Jialiang , Li Zhihao , Xi Lu , Chen Bing
Published: February 2023

Objective

To assess the diagnostic value of predictive models based on synthetic magnetic resonance imaging (syMRI), multiplexed sensitivity encoding (MUSE) sequences, and Breast Imaging Reporting and Data System (BI-RADS) in the differentiation of benign and malignant breast lesions.

Methods

Clinical and MRI data of 158 patients with breast lesions who underwent dynamic contrast-enhanced MRI (DCE-MRI), syMRI, and MUSE sequences between September 2019 and December 2020 were retrospectively collected. The apparent diffusion coefficient (ADC) values of MUSE and quantitative relaxation parameters (longitudinal and transverse relaxation times [T1, T2], and proton density [PD] values) of syMRI were measured, and the parameter variation values and change in their ratios were calculated. The patients were randomly divided into training (n = 111) and validation (n = 47) groups at a ratio of 7:3. A nomogram was built based on univariate and multivariate logistic regression analyses in the training group and was verified in the validation group. The discriminatory and predictive capacities of the nomogram were assessed by the receiver operating characteristic curve and area under the curve (AUC). The AUC was compared by DeLong test.

Results

In the training group, univariate analysis showed that age, lesion diameter, menopausal status, ADC, T2pre, PDpre, PDGd, T2Delta, and T2ratio were significantly different between benign and malignant breast lesions (P < 0.05). Multivariate logistic regression analysis showed that ADC and T2pre were significant variables (all P < 0.05) in breast cancer diagnosis. The quantitative model (model A: ADC, T2pre), BI-RADS model (model B), and multi-parameter model (model C: ADC, T2pre, BI-RADS) were established by combining the above independent variables, among which model C had the highest diagnostic performance, with AUC of 0.965 and 0.986 in the training and validation groups, respectively.

Conclusions

The prediction model established based on syMRI, MUSE sequence, and BI-RADS is helpful for clinical differentiation of breast tumors and provides more accurate information for individualized diagnosis.
Trefoil factor family proteins as potential diagnostic markers for mucinous invasive ovarian carcinoma
Authors: Werner Rönnerman Elisabeth , Pettersson Daniella , Nemes Szilárd , Dahm-Kähler Pernilla , Kovács Anikó , Karlsson Per , Parris Toshima Z. , Helou Khalil
Published: February 2023

Introduction

Ovarian cancer (OC) is the leading cause of gynecological cancer-related death. Of the main OC histologic subtypes, invasive mucinous carcinomas (MC) account for only 3% of OC cases and are frequently associated with favorable prognosis. Nevertheless, MCs differ greatly from the other OC histotypes in clinical, pathological, and biological behavior. However, the origin and molecular pathogenesis of MC are not yet fully understood. Therefore, identification of novel diagnostic markers could potentially facilitate early diagnosis of OC, particularly the MC histotype, thereby leading to the development of histotype-specific treatment regimens and improved survival rates.

Methods

In the present study, Trefoil factor gene family members (TFF1, TFF2 and TFF3) were identified as MC histotype-specific biomarkers using RNA sequencing (RNA-seq) data for 95 stage I-II OCs. The diagnostic value of TFF1, TFF2 and TFF3 was then evaluated by immunohistochemistry on 206 stage I-II OCs stratified by histotype (high-grade serous carcinoma [HGSC], endometrioid carcinoma [EC], clear cell carcinoma [CCC], and MC).

Results

We showed significantly elevated intracytoplasmic protein expression levels for TFF1, TFF2 and TFF3 in MC samples, thereby revealing an association between expression of Trefoil factor gene family members and the MC histotype. Taken together, these findings suggest that the TFF proteins may play a pivotal role in tumor initiation and progression for the MC histotype.

Conclusion

Taken together, these findings suggest that the TFF proteins may play a pivotal role in tumor initiation and progression for the MC histotype. Moreover, these novel histotype-specific diagnostic biomarkers may not only improve patient stratification of early-stage ovarian carcinomas but may also be candidates for the development of molecular targeted therapies.
Methylation-mediated silencing of EDN3 promotes cervical cancer proliferation, migration and invasion
Authors: Zhu Peng , Li Xiang , Liu Yujie , Xiong Jing , Yuan Ding , Chen Yan , Luo Lili , Huang Ju , Wang Binbin , Nie Quanfang , Wang Shuli , Dang Liying , Li Shu , Shu Yan , Zhang Wei , Zhou Honghao , Fan Lan , Li Qing
Published: February 2023

Cervical cancer (CC) remains one of the leading causes of cancer-related deaths worldwide. However, cervical cancer is preceded by the pre-malignant cervical intraepithelial neoplasia (CIN) that can last for up to 20 years before becoming malignant. Therefore, early screening is the key to prevent the progression of cervical lesions into invasive cervical cancer and decrease the incidence. The genes, down-regulated and hypermethylated in cancers, may provide potential drug targets for cervical cancer. In our current study, using the datasets from Gene Expression Omnibus (GEO) and the Cancer Genome Atlas (TCGA) databases, we found that endothelin 3 (EDN3) was downregulated and hypermethylated in cervical squamous cell carcinoma (CSCC). The further analysis in GSE63514 (n=128) dataset and in our samples (n=221) found that the expression of EDN3 was decreased with the degree of cervical lesions. Pyrosequencing was performed to evaluate 4 CpG sites of the EDN3 promoter region in our samples (n=469). The data indicated that the methylation level of EDN3 was increased with the degree of cervical lesions. EDN3 silencing mediated by methylation can be blocked by 5-Azacytidine (5-Aza), a DNA methyltransferase 1 (DNMT1) inhibitor, treatment in cervical cancer cell lines. Ethynyldeoxyuridine (EdU) assay, would-healing assay, clone formation assay and transwell assay were conducted to investigate the biological function of EDN3 in cervical cancer cell lines. The results of these experiments confirmed that overexpression of EDN3 could inhibit the proliferation, clone formation, migration and invasion of cervical cancer cells. EDN3 may provide potential biomarker and therapeutic target for CSCC.
The value of enhanced CT features and texture-signatures in assessing the inflammatory infiltration of pancreatic ductal adenocarcinoma
Authors: Wang Fangqing , Guo Hang , Li Shunjia , Xu Jianwei , Yu Dexin
Published: February 2023

Purpose

To explore the predictive value of computed tomography (CT) imaging features and CT-based texture analysis in assessing inflammatory infiltration in pancreatic ductal adenocarcinoma (PDAC).

Methods

A total of 43 patients with PDAC confirmed by surgical pathology were included in the study. The clinical, radiological, surgical, and pathological features of the patients were analyzed retrospectively using the chi-square test or Spearman’s correlation. Receiver operating characteristic (ROC) curves were utilized to assess the overall predictive ability of the tumor enhancement degree on triphasic contrast-enhanced CT images for the inflammatory infiltration degree in PDAC. Furthermore, all CT data were uploaded to the RadCloud platform for region of interest (ROI) delineation and feature extraction. Then, the Variance Threshold and SelectKBest algorithms were used to find the optimal CT features. Binary logistic regression was employed to analyze the selected features in all three contrast-enhanced CT phases, and regression equations were formulated. ROC analysis was performed to evaluate the predictive effectiveness of each equation.

Results

The analysis revealed a statistically significant correlation between the degree of differentiation and radiological findings such as necrosis and cystic degeneration, vascular invasion, and the presence of ascites (P < 0.05). The enhancement degree of the tumor in both the arterial and venous phases was significantly correlated with the inflammatory infiltration degree (P < 0.05); however, the areas under the ROC curve (AUCs) of arterial and venous enhancement were 0.570 and 0.542, respectively. Regression equations based on the texture features of triphasic contrast-enhanced tumors were formulated, and their AUCs were 0.982, 0.643, and 0.849.

Conclusion

Conventional radiological features are not significantly correlated with the degree of inflammatory infiltration in PDAC. The enhancement degrees in both the arterial phase and venous phase were statistically correlated with the inflammatory infiltration level but had poor predictive value. The texture features of PDAC on contrast-enhanced CT may show a better assessment value, especially in the arterial phase.
An integrated solution of deep reinforcement learning for automatic IMRT treatment planning in non-small-cell lung cancer
Authors: Wang Hanlin , Bai Xue , Wang Yajuan , Lu Yanfei , Wang Binbing
Published: February 2023

Purpose

To develop and evaluate an integrated solution for automatic intensity-modulated radiation therapy (IMRT) planning in non-small-cell lung cancer (NSCLC) cases.

Methods

A novel algorithm named as multi-objectives adjustment policy network (MOAPN) was proposed and trained to learn how to adjust multiple optimization objectives in commercial Eclipse treatment planning system (TPS), based on the multi-agent deep reinforcement learning (DRL) scheme. Furthermore, a three-dimensional (3D) dose prediction module was developed to generate the patient-specific initial optimization objectives to reduce the overall exploration space during MOAPN training. 114 previously treated NSCLC cases suitable for stereotactic body radiotherapy (SBRT) were selected from the clinical database. 87 cases were used for the model training, and the remaining 27 cases for evaluating the feasibility and effectiveness of MOAPN in automatic treatment planning.

Results

For all tested cases, the average number of adjustment steps was 21 ± 5.9 (mean ± 1 standard deviation). Compared with the MOAPN initial plans, the actual dose of chest wall, spinal cord, heart, lung (affected side), esophagus and bronchus in the MOAPN final plans reduced by 14.5%, 11.6%, 4.7%, 16.7%, 1.6% and 7.7%, respectively. The dose result of OARs in the MOAPN final plans was similar to those in the clinical plans. The complete automatic treatment plan for a new case was generated based on the integrated solution, with about 5-6 min.

Conclusion

We successfully developed an integrated solution for automatic treatment planning. Using the 3D dose prediction module to obtain the patient-specific optimization objectives, MOAPN formed action-value policy can simultaneously adjust multiple objectives to obtain a high-quality plan in a shorter time. This integrated solution contributes to improving the efficiency of the overall planning workflow and reducing the variation of plan quality in different regions and treatment centers. Although improvement is warranted, this proof-of-concept study has demonstrated the feasibility of this integrated solution in automatic treatment planning based on the Eclipse TPS.
Sex-specific radiomic features of L-[S-methyl-11C] methionine PET in patients with newly-diagnosed gliomas in relation to IDH1 predictability
Authors: Papp Laszlo , Rasul Sazan , Spielvogel Clemens P. , Krajnc Denis , Poetsch Nina , Woehrer Adelheid , Patronas Eva-Maria , Ecsedi Boglarka , Furtner Julia , Mitterhauser Markus , Rausch Ivo , Widhalm Georg , Beyer Thomas , Hacker Marcus , Traub-Weidinger Tatjana
Published: February 2023

Introduction

Amino-acid positron emission tomography (PET) is a validated metabolic imaging approach for the diagnostic work-up of gliomas. This study aimed to evaluate sex-specific radiomic characteristics of L-[S-methyl-11Cmethionine (MET)-PET images of glioma patients in consideration of the prognostically relevant biomarker isocitrate dehydrogenase (IDH) mutation status.

Methods

MET-PET of 35 astrocytic gliomas (13 females, mean age 41 ± 13 yrs. and 22 males, mean age 46 ± 17 yrs.) and known IDH mutation status were included. All patients underwent radiomic analysis following imaging biomarker standardization initiative (IBSI)-conform guidelines both from standardized uptake value (SUV) and tumor-to-background ratio (TBR) PET values. Aligned Monte Carlo (MC) 100-fold split was utilized for SUV and TBR dataset pairs for both sex and IDH-specific analysis. Borderline and outlier scores were calculated for both sex and IDH-specific MC folds. Feature ranking was performed by R-squared ranking and Mann-Whitney U-test together with Bonferroni correction. Correlation of SUV and TBR radiomics in relation to IDH mutational status in male and female patients were also investigated.

Results

There were no significant features in either SUV or TBR radiomics to distinguish female and male patients. In contrast, intensity histogram coefficient of variation (ih.cov) and intensity skewness (stat.skew) were identified as significant to predict IDH +/-. In addition, IDH+ females had significant ih.cov deviation (0.031) and mean stat.skew (-0.327) differences compared to IDH+ male patients (0.068 and -0.123, respectively) with two-times higher standard deviations of the normal brain background MET uptake as well.

Discussion

We demonstrated that female and male glioma patients have significantly different radiomic profiles in MET PET imaging data. Future IDH prediction models shall not be built on mixed female-male cohorts, but shall rely on sex-specific cohorts and radiomic imaging biomarkers.
Prediction of microvascular invasion in hepatocellular carcinoma based on preoperative Gd-EOB-DTPA-enhanced MRI: Comparison of predictive performance among 2D, 2D-expansion and 3D deep learning models
Authors: Wang Tao , Li Zhen , Yu Haiyang , Duan Chongfeng , Feng Weihua , Chang Lufan , Yu Jing , Liu Fang , Gao Juan , Zang Yichen , Luo Ziwei , Liu Hao , Zhang Yu , Zhou Xiaoming
Published: February 2023

Purpose

To evaluate and compare the predictive performance of different deep learning models using gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA)-enhanced MRI in predicting microvascular invasion (MVI) in hepatocellular carcinoma.

Methods

The data of 233 patients with pathologically confirmed hepatocellular carcinoma (HCC) treated at our hospital from June 2016 to June 2021 were retrospectively analyzed. Three deep learning models were constructed based on three different delineate methods of the region of interest (ROI) using the Darwin Scientific Research Platform (Beijing Yizhun Intelligent Technology Co., Ltd., China). Manual segmentation of ROI was performed on the T1-weighted axial Hepatobiliary phase images. According to the ratio of 7:3, the samples were divided into a training set (N=163) and a validation set (N=70). The receiver operating characteristic (ROC) curve was used to evaluate the predictive performance of three models, and their sensitivity, specificity and accuracy were assessed.

Results

Among 233 HCC patients, 109 were pathologically MVI positive, including 91 men and 18 women, with an average age of 58.20 ± 10.17 years; 124 patients were MVI negative, including 93 men and 31 women, with an average age of 58.26 ± 10.20 years. Among three deep learning models, 2D-expansion-DL model and 3D-DL model showed relatively good performance, the AUC value were 0.70 (P=0.003) (95% CI 0.57–0.82) and 0.72 (P<0.001) (95% CI 0.60–0.84), respectively. In the 2D-expansion-DL model, the accuracy, sensitivity and specificity were 0.7143, 0.739 and 0.688. In the 3D-DL model, the accuracy, sensitivity and specificity were 0.6714, 0.800 and 0.575, respectively. Compared with the 3D-DL model (based on 3D-ResNet), the 2D-DL model is smaller in scale and runs faster. The frames per second (FPS) for the 2D-DL model is 244.7566, which is much larger than that of the 3D-DL model (73.3374).

Conclusion

The deep learning model based on Gd-EOB-DTPA-enhanced MRI could preoperatively evaluate MVI in HCC. Considering that the predictive performance of 2D-expansion-DL model was almost the same as the 3D-DL model and the former was relatively easy to implement, we prefer the 2D-expansion-DL model in practical research.
Clinical features and enzyme replacement therapy in 10 children with Fabry disease
Authors: Li Qian , Wang Jing , Tian Minle , Yang Zhenle , Yu Lichun , Liu Suwen , Wang Cong , Wang Xiaoyuan , Sun Shuzhen
Published: February 2023

Objective

To summarize the clinical features, diagnosis and enzyme replacement therapy(ERT) of Fabry disease (FD) in children.

Methods

The clinical data, laboratory tests, genetic variations and treatment of 10 FD children diagnosed in Shandong Provincial Hospital from September 2020 to June 2022 were retrospectively analyzed.

Results

Among the 10 cases from 6 families, 7 patients were boys of 4 to 13 years of age, and 3 were girls of 12 to 15 years of age. There were 7 symptomatic patients, including 6 boys and 1 girl. All 7 patients presented with acral neuralgia. Five patients had little or no sweating. Five patients presented with cutaneous angiokeratoma. Two patients had abdominal pain. One patient developed joint symptoms. Four patients had corneal opacity. One patient had hearing loss; one patient had short stature. One patient had mild proteinuria and 1 patient had dysplasia of the right kidney with decreased eGFR (55.28 ml/min.1.73 m2). The left ventricular mass index was slightly elevated in 1 patient. Three patients had mild obstructive ventilatory dysfunction; a small amount of effusion in the intestinal space of the lower abdomen or mild fatty liver was found in 2 patients. Partial empty sella turcica in 1 patient. A total of 6 GLA gene variants were detected in 10 children, among which C.1059_1061delGAT (p.met353del) was a newly discovered mutation. Five children received ERT, of which 4 were treated with agalsidase beta and 1 was treated with agalsidase alpha. Only 1 patient had anaphylaxis. Lyso-GL-3 levels decreased significantly in the first 3 months of ERT initiation and remained relatively stable thereafter in 3 patients. The Lyso-GL-3 level was decreased, but renal impairment continued to progress in 1 patient treated with agalsidase alpha.

Conclusion

The clinical manifestations of FD in childhood are diverse, and it is necessary to make a definite diagnosis by combining family history, enzyme activity, biomarkers, gene testing and other indicators. Pedigree screening and high-risk population screening are helpful for early identification, early diagnosis and early treatment. No serious adverse reactions were found during the short-term treatment with agalsidase alpha and beta.
Establishment and validation of a predictive nomogram for the risk of premalignant lesions in children with choledochal cyst
Authors: Gao Ruyue , Ke Meng , Shi Jie , Zhang Yandong , Zou Jizhen , Diao Mei , Li Long
Published: February 2023

Background

Choledochal cyst (CDC) increases the risk (2.5%–30%) of malignancy. Metaplasia and dysplasia have been recognized as premalignant lesions among CDCs. This study aimed to evaluate the risk factors of metaplasia and dysplasia in CDC children.

Methods

Two hundred and ten CDC children who underwent cyst excision and Roux-en-Y hepaticojejunostomy at our institution between July 2020 and November 2021 were included and randomly divided into the training set and validation set. Univariate and multivariate logistic regression analysis were used to identify independent risk factors of premalignant lesions in the training set and build a predictive nomogram. The performance and discriminatory abilities of the nomogram were further assessed and validated in the validation set.

Results

Of the 210 CDC children, 78 (37.1%) patients developed premalignant lesions. Age (OR, 1.011, 95%CI, 1.000–1.022, P = 0.046), symptoms duration (OR, 1.021, 95%CI, 1.001–1.042, P = 0.036), cyst diameter (OR, 1.737, 95%CI, 1.328–2.273, P < 0.001), recurrent attacks of biliary pancreatitis (OR, 3.653, 95%CI, 1.205–11.076, P = 0.022), and biliary operation history (OR, 5.860, 95%CI, 1.268–27.084, P = 0.024) were identified as independent risk factors. Based on these predictors, a predictive nomogram was generated. The AUC of the nomogram was 0.873 in the training set and 0.793 in the validation set, indicating that it was robust and well calibrated.

Conclusions

A novel nomogram to the individualized risk of premalignant lesions in CDC children was successfully built, on the basis of age, symptoms duration, cyst diameter, recurrent attacks of biliary pancreatitis, and biliary operation history. This nomogram, combined with the final pathological results, can help clinicians to develop more efficient follow-up strategies for the high-risk children with CDC.
Heat stress responses vary during floret development in European spring barley cultivars
Authors: Callens Cindy , Fernandez-Goméz José , Tucker Matthew R. , Zhang Dabing , Wilson Zoe A.
Published: February 2023

The Poaceae, or grasses, include many agriculturally important cereal crops such as rice (Oryza sativa), maize (Zea mays), barley (Hordeum vulgare) and bread wheat (Triticum aestivum). Barley is a widely grown cereal crop used for stock feed, malting and brewing. Abiotic stresses, particularly global warming, are the major causes of crop yield losses by affecting fertility and seed set. However, effects of heat stress on reproductive structures and fertility in barley have not been extensively investigated. In this study we examined three commercial European spring barley varieties under high temperature conditions to investigate the effects on floret development. Using a combination of fertility assays, X-ray micro computed tomography, 3-dimensional modelling, cytology and immunolabelling, we observed that male reproductive organs are severely impacted by increased temperature, while the female reproductive organs are less susceptible. Importantly, the timing of stress relative to reproductive development had a significant impact on fertility in a cultivar-dependent manner, this was most significant at pollen mitosis stage with fertility ranged from 31.6-56.0% depending on cultivar. This work provides insight into how heat stress, when applied during male pollen mother cell meiosis and pollen mitosis, affects barley fertility and seed set, and also describes complementary invasive and non-invasive techniques to investigate floret development. This information will be used to identify and study barley cultivars that are less susceptible to heat stress at specific stages of floral development.
Evaluationof compact air-induction flat fan nozzles for herbicide applications: Spray drift and biological efficacy
Authors: Wang Shilin , Li Xinjie , Nuyttens David , Zhang Lanting , Liu Yajia , Li Xue
Published: February 2023

Nozzles are the most critical component of a sprayer for pesticide applications. Recently, air-induction nozzles and twin flat-fan air-induction nozzles have started to be used for herbicide applications. In order to evaluate the potential of compact air-induction nozzles for herbicide spraying, this paper compares the effects of air-induction nozzles and standard flat-fan nozzles on spray atomization, deposition, drift, and weed control efficacy in maize and wheat. Droplet spectra were measured by a laser particle size analyzer, and drift potential values were determined using a drift test bench (ISO 22401). A field study was conducted to compare the spray drift and biological efficacy between Lechler standard flat-fan nozzles and compact air-induction nozzles including different nozzle sizes. In the range from 0.2 to 0.4 MPa, the droplet size classes of the LU and ST nozzles were very similar and ranged from fine to very fine, while the droplets of the air-induction nozzles IDK and IDKT were medium or coarse depending on the spray pressure and nozzle size. The drift potential trials showed that the droplet size characteristics, mainly V100, are strongly linked with the drift reduction potential. Both drift potential and field results showed that the compact air-induction nozzles had a good performance in drift reduction. In terms of weed control biological efficacy, there were no significant differences between standard flat-fan nozzles and air-induction nozzles. In all cases, the efficacy values were above 80% both in maize and in wheat. In conclusion, air-induction nozzles are recommended for herbicide applications as they provide good biological efficacy while significantly reducing the amount of spray drift, which is of great significance for the protection of the environment and the surrounding sensitive crops.
Effect of temperature and humidity on dynamics and transmission of Pseudomonas amygdali pv. lachrymans aerosols
Authors: Chai Ali , Yuan Lifang , Li Xin , Li Lei , Shi Yanxia , Xie Xuewen , Li Baoju
Published: February 2023

Cucumber angular leaf spot (ALS) disease, caused by Pseudomonas amygdali pv. lachrymans (Pal), is an emerging disease with a high incidence that causes severe damage to cucumber worldwide. Bacterial aerosols play a crucial role in the epidemiology of greenhouse ALS disease. However, little is known about the influence of temperature and relative humidity (RH) on the dynamics of Pal in aerosols. A study was conducted to investigate the relationships between the concentration of Pal aerosols and their dependence on temperature and RH in aerosol chambers and greenhouses. The results demonstrated that temperature and RH are both significant factors influencing the release amount, survival time and infectivity of Pal in aerosols, while RH has a greater influence on particle size than temperature across the range of conditions tested. The release amount and survival time of Pal in aerosols under high RH (95%) and low temperature (≤ 25°C) conditions were significantly higher than those under low RH (35%) and high temperature (35°C) conditions. The highest release amount of Pal aerosol (96 CFU/m3) and highest survival rate (98.41%) were found at 18°C and 95% RH, while the highest disease index (DI = 60.9) caused by Pal aerosol was found at 25°C and 95% RH. In addition, Pal aerosols presented a larger diameter (4.7->7.0 μm) under high RH (95% RH) than under dry conditions (≤ 65% RH). These findings will play a crucial role in elucidating the influence of environmental parameters on the dynamics and transmission of Pal in aerosols. Based on our findings, preliminary recommendations for controlling airborne Pal spread involve controlling air temperature and RH, which will contribute to the effective alleviation and control of cucumber ALS disease.
Discovery of a cytochrome P450 enzyme catalyzing the formation of spirooxindole alkaloid scaffold
Authors: Nguyen Tuan-Anh M. , Grzech Dagny , Chung Khoa , Xia Zhicheng , Nguyen Trinh-Don , Dang Thu-Thuy T.
Published: February 2023

Spirooxindole alkaloids feature a unique scaffold of an oxindole ring sharing an atom with a heterocyclic moiety. These compounds display an extensive range of biological activities such as anticancer, antibiotics, and anti-hypertension. Despite their structural and functional significance, the establishment and rationale of the spirooxindole scaffold biosynthesis are yet to be elucidated. Herein, we report the discovery and characterization of a cytochrome P450 enzyme from kratom (Mitragyna speciosa) responsible for the formation of the spirooxindole alkaloids 3-epi-corynoxeine (3R, 7R) and isocorynoxeine (3S, 7S) from the corynanthe-type (3R)-secoyohimbane precursors. Expression of the newly discovered enzyme in Saccharomyces cerevisiae yeast allows for the efficient in vivo and in vitro production of spirooxindoles. This discovery highlights the versatility of plant cytochrome P450 enzymes in building unusual alkaloid scaffolds and opens a gateway to access the prestigious spirooxindole pharmacophore and its derivatives.
Research or teaching? That is the problem: A narrative inquiry into a Chinese college English teacher’s cognitive development in the teaching-research nexus
Authors: Ni Hui , Wu Xinxin
Published: February 2023

This narrative inquiry traces a Chinese college English teacher’s cognitive transformation in the teaching-research nexus over a 4-year time period from the perspective of sociocultural theory (SCT). Several stories were narrated based on the data from reflective journals, literature reading notes, and interaction notes to show how the participant walked out of the teaching-research contradiction and finally achieved professional development. The findings indicate that the college English teacher’s perception of the research-teaching nexus developed when she actively exercised her agency to take part in social interactions in a supportive sociocultural environment with the regulation of appropriate mediational tools. The conceptual progress in the research-teaching nexus finally contributed to her professional development in both teaching and research. The findings of this study have implications for non-key universities and their academics on how to effectively promote teaching-research integration.
Longitudinal change of inhibitory control functional connectivity associated with the development of heavy alcohol drinking
Authors: Antón-Toro Luis F. , Shpakivska-Bilan Danylyna , Del Cerro-León Alberto , Bruña Ricardo , Uceta Marcos , García-Moreno Luis M. , Maestú Fernando
Published: February 2023

Introduction

Heavy drinking (HD) prevalent pattern of alcohol consumption among adolescents, particularly concerning because of their critical vulnerability to the neurotoxic effects of ethanol. Adolescent neurodevelopment is characterized by critical neurobiological changes of the prefrontal, temporal and parietal regions, important for the development of executive control processes, such as inhibitory control (IC). In the present Magnetoencephalography (MEG) study, we aimed to describe the relationship between electrophysiological Functional Connectivity (FC) during an IC task and HD development, as well as its impact on functional neuromaturation.

Methods

We performed a two-year longitudinal protocol with two stages. In the first stage, before the onset of HD, we recorded brain electrophysiological activity from a sample of 67 adolescents (mean age = 14.6 ± 0.7) during an IC task. Alcohol consumption was measured using the AUDIT test and a semi-structured interview. Two years later, in the second stage, 32 of the 67 participants (mean age 16.7 ± 0.7) completed a similar protocol. As for the analysis in the first stage, the source-space FC matrix was calculated, and then, using a cluster-based permutation test (CBPT) based on Spearman’s correlation, we calculated the correlation between the FC of each cortical source and the number of standard alcohol units consumed two years later. For the analysis of longitudinal change, we followed a similar approach. We calculated the symmetrized percentage change (SPC) between FC at both stages and performed a CBPT analysis, analyzing the correlation between FC change and the level of alcohol consumed in a regular session.

Results

The results revealed an association between higher beta-band FC in the prefrontal and temporal regions and higher consumption years later. Longitudinal results showed that greater future alcohol consumption was associated with an exacerbated reduction in the FC of the same areas.

Discussion

These results underline the existence of several brain functional differences prior to alcohol misuse and their impact on functional neuromaturation.
Effects of academic self-regulation on procrastination, academic stress and anxiety, resilience and academic performance in a sample of Spanish secondary school students
Authors: Ragusa Antonio , González-Bernal Jeronimo , Trigueros Ruben , Caggiano Valeria , Navarro Noelia , Minguez-Minguez Luis A. , Obregón Ana I. , Fernandez-Ortega Carmen
Published: February 2023

Introduction

One of the biggest challenges facing students today is procrastination, which is closely related to stress, anxiety and, in the most severe cases, depression. The aim of the present study is to analyze the influence of academic self-regulation on procrastination, academic anxiety and stress, academic resilience and academic performance.

Method

The sample consisted of 991 high school students aged between 16 and 19 years (M = 17.25; SD = 3.45). A structural equation model was used to analyze the relationship between the study variables.

Results

Analyzes showed that academic self-regulation negatively predicted procrastination. In turn, procrastination positively predicted academic stress and anxiety. However, resilience exerted a protective influence by being positively related to academic stress and anxiety. Finally, resilience positively predicted academic performance, whereas stress and anxiety negatively predicted academic performance.

Conclusion

Thus, the importance of generating strategies for students to learn to self-regulate in academic contexts, manage emotions, foster motivation and develop strategies to help them overcome the vicissitudes they face is emphasized.
The role of emotion and reflection in the development of student teachers’ self-efficacy when analyzing video lessons
Authors: Schlosser Anne , Paetsch Jennifer
Published: February 2023

Introduction

Teachers’ self-efficacy is an important indicator of student teachers’ preparedness for teaching. Interventions using video lessons are effective in increasing student teachers’ self-efficacy. However, there is a lack of research on emotional and reflective processes in the context of video-based interventions.

Methods

The present study examined emotions and reflection as well as their effects on changes in self-efficacy in a video-based intervention. A total of 159 student teachers participated in the study. The participants were randomly assigned to three groups: Two groups analyzed video lessons in which group roup one received open-ended observation tasks (ig1) and group two received structured observation tasks (ig2). Participants in the control group (cg) analyzed text-based case studies with open-ended observation tasks.

Results

The results show that self-efficacy increased with medium effect size (d = 0.68) in video group two (ig2), whose members analyzed videos using structured observation tasks but not in video group one (ig1), whose members analyzed open-ended observation tasks, and in the control group. In addition, there were significant relations between positive arousal and reflection. Finally, regression analyses showed that reflection was a significant predictor for changes in self-efficacy, whereas no significant effect of emotional arousal was detected.

Discussion

In conclusion, the findings of this study indicate that video-based interventions with structured observation tasks increased self-efficacy among student teachers. Furthermore, the findings provide novel evidence on the association between reflection, self-efficacy and emotion in video-based interventions in teacher education.
The difficulty of oral speech act production tasks in second language pragmatics testing
Authors: Huang Weiying , Lu Xiaofei
Published: February 2023

This study examined the relative difficulty of oral speech act production tasks involving eight different types of speech acts for Chinese English as a foreign language (EFL) learners and the effects of three contextual variables, namely, power, social distance, and imposition, on such difficulty. Eight Oral Discourse Completion Task items, each representing a unique combination of the three contextual variables, were designed for each speech act. Eighty Chinese EFL learners responded to these items and their responses were rated for appropriateness by two native-speaking college English instructors. A Many-facet Rasch Measurement analysis suggested that the eight speech acts can be ordered by ascending difficulty as follows: Thank, Request, Suggestion, Disagreement, Invitation, Refusal, Offer, and Apology. Significant effects on performance scores were found for the interaction between each of the three contextual variables and speech act, and the specific effects observed varied by speech act. The implications of our findings for L2 pragmatics testing are discussed.
Intergenerational and intragenerational preferences in a developing country to avoid climate change
Authors: Alvi Shahzad , Salman Verda , Bibi Fazal Un Nisa , Sarwar Naima
Published: February 2023

Intergenerational and intragenerational approaches to climate change take into account the actions taken by the current generation to maintain or improve the climate, which is advantageous to both the present and future generations. Climate-friendly initiatives primarily benefit future generations, with current generations receiving lesser benefits. Self-interest can hinder the management of shared resources, as seen in the “tragedy of the commons” concept, where individuals benefit from defecting, but society bears the consequences of it. This study used three different time horizons to determine the inter- and intra-generational preferences of groups of human subjects for preventing hazardous climate change. We looked at how groups of participants responded in scenarios that varied in motivation, income, social pressure, and learning opportunities. For this purpose, we conducted two group experiments framed around climate change where participants could choose to cooperate for a noble cause: tree plantations. Its rewards are delayed by several years and probably a few decades (intergenerational discounting), where future generations will be the big beneficiaries. There were two more options: the first one delayed the reward by 1 week, and the second was delayed by seven weeks (intragenerational discounting). We found that intergenerational discounting was high when the groups had free will and motivation. Further, it is revealed that having more money does not play a significant positive role in long-term climate sustainability in a developing country; however, it does, but not as much as motivation and free will do.
Triglyceride glucose-body mass index and the risk of progression to diabetes from prediabetes: A 5-year cohort study in Chinese adults
Authors: Han Yong , Hu Haofei , Li Qiming , Deng Zhe , Liu Dehong
Published: February 2023

Objective

Evidence regarding the relationship between the triglyceride glucose-body mass index (TyG-BMI) and the risk of progression from prediabetes to diabetes remains limited. Our study aimed to investigate the relationship between them in patients with prediabetes.

Methods

In this retrospective cohort study, data were collected from 25,279 patients with prediabetes who received health checks between 2010 and 2016. We used a Cox proportional-hazards regression model to examine the relationship between TyG-BMI and diabetes risk. We used Cox proportional hazards regression with cubic spline functions and smooth curve fitting to identify the nonlinear relationship between them. In addition, A series of sensitivity and subgroup analyses were also conducted.

Results

The mean age of the included participants was 49.29 ± 13.82 years old, and 1,6734 (66.2%) were male. The mean TyG-BMI was 219.47. The median follow-up time was 2.89 years, and 2,687 (10.63%) individuals had a final diagnosis of diabetes. After adjusting for covariates, TyG-BMI was positively linked with incident diabetes in patients with prediabetes (HR = 1.011, 95%CI 1.010–1.012). TyG-BMI had a non-linear connection with diabetes risk, and its inflection point was 231.66. Right and left effects sizes (HR) at the inflection point were 1.017 (95%CI:1.014–1.019) and 1.007 (95%CI:1.005–1.009), respectively. The sensitivity analysis demonstrated the robustness of these results.

Conclusion

This study demonstrated a positive, non-linear relationship between the TyG-BMI and diabetes risk in Chinese patients with prediabetes. When the TyG-BMI was <231.66, there was a significant positive association between TyG-BMI and the risk of progression from prediabetes to diabetes. This study serves as a reference to promote clinical consultation and optimize diabetes prevention decisions for patients with prediabetes.
An innovative framework to determine the implementation level of personalized medicine: A systematic review
Authors: Aguilera-Cobos Lorena , García-Sanz Patricia , Rosario-Lozano María Piedad , Claros M. Gonzalo , Blasco-Amaro Juan Antonio
Published: February 2023

Background

Personalized medicine (PM) is now the new frontier in patient care. The application of this new paradigm extends to various pathologies and different patient care phases, such as diagnosis and treatment. Translating biotechnological advances to clinical routine means adapting health services at all levels is necessary.

Purpose

This article aims to identify the elements for devising a framework that will allow the level of PM implementation in the country under study to be quantitatively and qualitatively assessed and that can be used as a guideline for future implementation plans.

Methods

A systematic review was conducted per the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. The research question was: What are the domains for determining the level of implementation of PM at the national level? The domains for assessing the degree of PM implementation, which would form the framework, were established.

Results

19 full-text studies that met the inclusion criteria were peer-selected in the systematic review. From all the studies that were included, 37 elements—encompassed in 11 domains—were extracted for determining the degree of PM implementation. These domains and their constituent elements comprise the qualitative and quantitative assessment framework presented herein. Each of the elements can be assessed individually. On the other hand, the domains were standardized to all have the same weight in an overall assessment.

Conclusions

A framework has been developed that takes a multi-factorial approach to determine the degree of implementation of PM at the national level. This framework could also be used to rank countries and their implementation strategies according to the score they receive in the application of the latter. It could also be used as a guide for developing future national PM implementation strategies.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022338611, Identifier: CRD42022338611.
Bee diversity on urban rooftop food gardens
Authors: Riehn Jordyn K. , Fogel Nina S. , Hathaway Jordan N. , Camilo Gerardo R.
Published: February 2023

Green infrastructure on rooftops in urban areas can enhance important ecosystem services. In addition to mitigating water runoff and regulating building temperatures, green roofs can provide food and nesting resources for wildlife. Rooftop gardens can also be utilized to cultivate food crops, giving them the potential to attenuate instances of food insecurity which are commonplace in many urban areas. Given that many crops depend on bee pollination, it is imperative to characterize the rooftop bee community. Therefore, we sampled three urban food roofs near downtown St. Louis, MO during the 2017 and 2018 growing seasons. We found 38 bee species utilizing the rooftop farms. We then compared the rooftop bee communities to those of two nearby ground-level community gardens. The roofs had lower abundances of Bombus species than the ground-level sites while non-native cavity nesters in the genus Megachile were overrepresented. These results indicate that the rooftop bee communities represent a nested subset of the species found at ground level. This has important implications for the management of green roofs for growing crops, as this shift in bee community structure may play a role in determining which crops can be adequately supported with pollination services.
A Reef Like no Other: The Great Southern Reef
Authors: Freitas Cátia , O’Brien Madi , Andrews Stefan , Francis Prue
Published: February 2023

Under the water, along the southern coastline of Australia, lies the Great Southern Reef—a reef that many people have never heard about. The water is colder than that of tropical reefs and instead of being built by corals, it is mainly formed by a specific type of brown seaweed known as kelp. Just like trees on land, kelp grow and cover large areas, forming underwater forests that are home for many kinds of fish, molluscs, crustaceans, and seaweed species. However, the Great Southern Reef is facing serious threats, mainly because of rising ocean temperatures. When the seawater becomes too warm, kelp can disappear, endangering the marine creatures that live in the reef ecosystem. It is difficult to protect something if people do not know it exists! This is why we need to raise awareness of how valuable this reef is. Can you help us spread the word?
Identification of wild edible plants used by the Orang Asli, indigenous peoples of the Malay Peninsula
Authors: Tharmabalan Rachel Thomas
Published: February 2023

Background

This research focused on the different varieties of wild edible plants (WEPs) actively utilized by the Orang Asli of Malaysia and their medicinal values. The Orang Asli have been relying on these plants for their sustenance and wellbeing. However, with environmental degradation, many of these wild edibles are slowly becoming sparse, which then has a spillover effect and has resulted in the erasure of knowledge. Therefore, this research paper also aims to highlight the naming system utilized by the Orang Asli, the gender/age gap and the medicinal properties of these wild edible plants.

Methods

This research was conducted to identify the important wild edibles used among the Orang Asli, using both semi-structured and field interviews, as well as ethnobotanical appraisal methods. With the assistance of 24 informants, a total of nine wild edible plants were identified based on their importance as food and medicine for the Orang Asli groups in three different Semai settlements.

Results and discussion

From the results obtained, the wild edibles identified in the study have potential medicinal value and have been used by the Orang Asli for many generations. Also, based on my results, older Orang Asli can potentially serve as a repository of knowledge of wild edible plants within their settlements, as they were more informed than young people. However, there is little remaining knowledge of their medicinal uses in Telimau; elder women retain more knowledge than elder men in Bukit Terang, and the greatest knowledge retention for both young and old is seen in the more remote settlement of Kampung Sat.

Conclusion

This research documents the medicinal uses of these wild edibles, and may provide a starting point for further studies on these species and encourage conservation initiatives, in addition to helping safeguard the indigenous knowledge and the distinct cultural identities of the Orang Asli.