bluuebunny/crossref_metadata_2025 huggingface dataset:
Case report: Near-fatal hypermagnesemia resulting from the use of Epsom salts in a patient with normal renal function
Authors: Si Gui-Fei , Ge Yu-Xin , Lv Xiao-Pan , Li Yu-Quan , Chen Xue-Mei , Yuan Xue-Min
Published: July 2024

Hypermagnesemia commonly occurs in patients with renal dysfunction. Diagnosing hypermagnesemia represents a challenge due to its rarity and the absence of routine monitoring of magnesium levels. Furthermore, the lack of awareness among clinicians regarding this uncommon condition frequently leads to delayed diagnoses. Few patients survive with a serum magnesium level exceeding 7 mmol/L. This article presents a case study of near-fatal hypermagnesemia resulting from the oral administration of Epsom salts in a patient with normal renal function. A 60-year-old female presented to the gastroenterology department on Oct. 6, 2023, with a 3-day history of black stools. She underwent subtotal gastrectomy in 2005 and has a stable history of nephrotic syndrome. To investigate the cause of her bleeding, electronic gastroscopy and colonoscopy were scheduled for Oct. 11, 2023. She experienced a sudden loss of consciousness 30 min after the ingestion of Epsom salts. The attending physician suspected a severe magnesium poisoning. She was promptly administered calcium gluconate, underwent tracheal intubation with ambu bag ventilation, and received early continuous renal replacement therapy (CRRT). Swift diagnosis and CRRT contributed to a reduction in her serum magnesium levels from an initial 8.71 mmol/L to 1.35 mmol/L, leading to a remarkable improvement in the toxic symptoms associated with hypermagnesemia. Subsequently, she was managed in the gastroenterology department, with gastroscopy revealing bleeding from the gastrointestinal anastomotic ulcer. Following conservative treatments including acid suppression, stomach protection, and hemostasis, her symptoms improved, and she was successfully discharged. This study aims to alert clinicians to the possibility of hypermagnesemia in individuals with normal renal function. Physicians should exercise caution when prescribing Epsom salts to patients with underlying gastrointestinal conditions. If necessary, alternative drug therapies may be considered to mitigate the risk of hypermagnesemia. Timely intervention is pivotal in averting life-threatening complications linked to hypermagnesemia.
Quantitative mass spectrometry analysis of the injured proximal and distal human digital nerve ends
Authors: Frostadottir Drifa , Welinder Charlotte , Perez Raquel , Dahlin Lars B.
Published: July 2024

Introduction

Proteomic analysis of injured human peripheral nerves, particularly focusing on events occurring in the proximal and distal nerve ends, remains relatively underexplored. This study aimed to investigate the molecular patterns underlying a digital nerve injury, focusing on differences in protein expression between the proximal and distal nerve ends.

Methods

A total of 26 human injured digital nerve samples (24 men; 2 women; median age 47 [30–66] years), harvested during primary nerve repair within 48 h post-injury from proximal and distal nerve ends, were analyzed using mass spectrometry.

Results

A total of 3,914 proteins were identified, with 127 proteins showing significant differences in abundance between the proximal and the distal nerve ends. The downregulation of proteins in the distal nerve end was associated with synaptic transmission, autophagy, neurotransmitter regulation, cell adhesion and migration. Conversely, proteins upregulated in the distal nerve end were implicated in cellular stress response, neuromuscular junction stability and muscle contraction, neuronal excitability and neurotransmitter release, synaptic vesicle recycling and axon guidance and angiogenesis.

Discussion

Investigation of proteins, with functional annotations analysis, in proximal and the distal ends of human injured digital nerves, revealed dynamic cellular responses aimed at promoting tissue degeneration and restoration, while suppressing non-essential processes.
Digestible indispensable amino acid score (DIAAS): 10 years on
Authors: Moughan Paul J. , Lim Wen Xin Janice
Published: July 2024

The objective of the review is to revisit the findings of the 2011 Food and Agriculture Organization of the United Nations (FAO) Expert Consultation on Dietary Protein Quality Evaluation in Human Nutrition, and to report on progress on uptake of the findings. It is evident that since 2011 there has been a concerted research effort to enhance an understanding of the protein quality of foods. The validity of the growing pig ileal protein digestibility assay has been confirmed and numerous studies reported using the growing pig as a model to give true ileal amino acid digestibility values for foods as consumed by humans. This has allowed for the determination of digestible indispensable amino acid scores (DIAAS) for a range of foods. A new non-invasive true ileal amino acid digestibility assay in humans which can be applied in different physiological states, called the dual-isotope assay, has been developed and applied to determine the DIAAS values of foods. It is concluded that DIAAS is currently the most accurate score for routinely assessing the protein quality rating of single source proteins. In the future, the accuracy of DIAAS can be enhanced by improved information on: the ideal dietary amino acid balance including the ideal dispensable to indispensable amino acid ratio; dietary indispensable amino acid requirements; effects of processing on ileal amino acid digestibility and lysine bioavailability. There is a need to develop rapid, inexpensive in vitro digestibility assays. Conceptual issues relating DIAAS to food regulatory claims, and to holistic indices of food nutritional and health status are discussed. The first recommendation of the 2011 Consultation regarding treating each indispensable amino acid as an individual nutrient has received little attention. Consideration should be given to providing food label information on the digestible contents of specific indispensable amino acids.
Identification of biological signatures of cruciferous vegetable consumption utilizing machine learning-based global untargeted stable isotope traced metabolomics
Authors: Bouranis John A. , Ren Yijie , Beaver Laura M. , Choi Jaewoo , Wong Carmen P. , He Lily , Traber Maret G. , Kelly Jennifer , Booth Sarah L. , Stevens Jan F. , Fern Xiaoli Z. , Ho Emily
Published: July 2024

In recent years there has been increased interest in identifying biological signatures of food consumption for use as biomarkers. Traditional metabolomics-based biomarker discovery approaches rely on multivariate statistics which cannot differentiate between host- and food-derived compounds, thus novel approaches to biomarker discovery are required to advance the field. To this aim, we have developed a new method that combines global untargeted stable isotope traced metabolomics and a machine learning approach to identify biological signatures of cruciferous vegetable consumption. Participants consumed a single serving of broccoli (n = 16), alfalfa sprouts (n = 16) or collard greens (n = 26) which contained either control unlabeled metabolites, or that were grown in the presence of deuterium-labeled water to intrinsically label metabolites. Mass spectrometry analysis indicated 133 metabolites in broccoli sprouts and 139 metabolites in the alfalfa sprouts were labeled with deuterium isotopes. Urine and plasma were collected and analyzed using untargeted metabolomics on an AB SCIEX TripleTOF 5,600 mass spectrometer. Global untargeted stable isotope tracing was completed using openly available software and a novel random forest machine learning based classifier. Among participants who consumed labeled broccoli sprouts or collard greens, 13 deuterium-incorporated metabolomic features were detected in urine representing 8 urine metabolites. Plasma was analyzed among collard green consumers and 11 labeled features were detected representing 5 plasma metabolites. These deuterium-labeled metabolites represent potential biological signatures of cruciferous vegetables consumption. Isoleucine, indole-3-acetic acid-N-O-glucuronide, dihydrosinapic acid were annotated as labeled compounds but other labeled metabolites could not be annotated. This work presents a novel framework for identifying biological signatures of food consumption for biomarker discovery. Additionally, this work presents novel applications of metabolomics and machine learning in the life sciences.
Evaluation of Ethos intelligent optimization engine for left locally advanced breast cancer
Authors: Prunaretty Jessica , Lopez Laura , Cabaillé Morgane , Bourgier Céline , Morel Aurélie , Azria David , Fenoglietto Pascal
Published: July 2024

Purpose

To evaluate the feasibility to use a standard Ethos planning template to treat left-sided breast cancer with regional lymph nodes.

Material/Methods

The tuning cohort of 5 patients was used to create a planning template. The validation cohort included 15 patients treated for a locally advanced left breast cancer randomly enrolled. The Ethos planning template was tuned using standard 3 partial arc VMAT and two collimator rotation configurations: 45/285/345° and 30/60/330°. Re-planning was performed automatically using the template without editing. The study was conducted with a schedule of 42.3 Gy in 18 fractions to the breast/chestwall, internal mammary chain (IMC) and regional lymph nodes (“Nodes”). The PTV was defined as a 3D extension of the CTV with a margin of 7 mm, excluding the 5mm below the skin. The manual treatment plans were performed using Eclipse treatment planning system with AAA and PO algorithms (v15.6) and a manual arc VMAT configuration and imported in Ethos TPS (v1.1) for a dose calculation with Ethos Acuros algorithm. The automated plans were compared with the manual plans using PTV and CTV coverage, homogeneity and conformity indices (HI and CN) and doses to organs at risk (OAR) via DVH metrics. For each plan, the patient quality assurance (QA) were performed using Mobius3D and gamma index. Finally, two breast radiation oncologists performed a blinded assessment of the clinical acceptability of each of the three plans (manual and automated) for each patient.

Results

The manual and automated plans provided suitable treatment planning as regards dose constraints. The dosimetric comparison showed the CTV_breast D99% were significantly improved with both automated plans (p< 0,002) while PTV coverage was comparable. The doses to the organs at risk were equivalent for the three plans. Concerning treatment delivery, the Ethos-45° and Ethos-30° plans led to an increase in MUs compared to the manual plans, without affecting the beam on time. The average gamma index pass rates remained consistently above 98% regardless of the type of plan utilized. In the blinded evaluation, clinicians 1 and 2 assessed 13 out of 15 plans for Ethos 45° and 11 out of 15 plans for Ethos 30° as clinically acceptable.

Conclusion

Using a standard planning template for locally advanced breast cancer, the Ethos TPS provided automated plans that were clinically acceptable and comparable in quality to manually generated plans. Automated plans also dramatically reduce workflow and operator variability.
Lycium ruthenicum water extract preserves retinal ganglion cells in chronic ocular hypertension mouse models
Authors: Liu Jinfeng , Zhou Lina , Wu Xueping , Chen Zihang , Zheng Xiaofei , Wang Huajun , So Kwok Fai , Ma Lan , Wang Jiantao , Chiu Kin
Published: July 2024

Lycium ruthenicum Murray (LR), known as “black goji berry” or “black wolfberry”, is widely utilized in chinese herbal medicine. LR fruit showed its antioxidant and/or anti-inflammation activity in treating cardiac injury, experimental colitis, nonalcoholic fatty liver disease, fatigue, and aging. Glaucoma is the leading cause of irreversible blindness. Besides elevated intraocular pressure (IOP), oxidative stress and neuroinflammation were recognized to contribute to the pathogenesis of glaucoma. This study investigated the treatment effects of LR water extract (LRE) on retinal ganglion cells (RGCs) threatened by sustained IOP elevation in a laser-induced chronic ocular hypertension (COH) mouse model and the DBA/2J mouse strain. The antioxidation and anti-inflammation effects of LRE were further tested in the H2O2-challenged immortalized microglial (IMG) cell line in vitro. LRE oral feeding (2 g/kg) preserved the function of RGCs and promoted their survival in both models mimicking glaucoma. LRE decreased 8-hydroxyguanosine (oxidative stress marker) expression in the retina. LRE reduced the number of Iba-1+ microglia in the retina of COH mice, but not in the DBA/2J mice. At the mRNA level, LRE reversed the COH induced HO-1 and SOD-2 overexpressions in the retina of COH mice. Further in vitro study demonstrated that LRE pretreatment to IMG cells could significantly reduce H2O2 induced oxidative stress through upregulation of GPX-4, Prdx-5, HO-1, and SOD-2. Our work demonstrated that daily oral intake of LRE can be used as a preventative/treatment agent to protect RGCs under high IOP stress probably through reducing oxidative stress and inhibiting microglial activation in the retina.
The signaling pathways of selected traditional Chinese medicine prescriptions and their metabolites in the treatment of diabetic cardiomyopathy: a review
Authors: Li Wencan , Liu Xiang , Liu Zheng , Xing Qichang , Liu Renzhu , Wu Qinxuan , Hu Yixiang , Zhang Jiani
Published: July 2024

Diabetic cardiomyopathy (DCM) is a myocardial-specific microvascular disease caused by diabetes that affects the structure and function of the heart and is considered to be the leading cause of morbidity and death in patients with diabetes. Currently, there is no specific treatment or preventive drug for DCM, and there is an urgent need to develop new drugs to treat DCM. Traditional Chinese medicine (TCM) has rich experience in the treatment of DCM, and its characteristics of multi-target, multi-pathway, multi-component, and few side effects can effectively deal with the complexity and long-term nature of DCM. Growing evidence suggests that myocardial fibrosis, inflammation, oxidative stress, apoptosis, cardiac hypertrophy, and advanced glycation end product deposition were the main pathologic mechanisms of DCM. According to the pathological mechanism of DCM, this study revealed the potential of metabolites and prescriptions in TCM against DCM from the perspective of signaling pathways. The results showed that TGF-β/Smad, NF-κB, PI3K/AKT, Nrf2, AMPK, NLRP3, and Wnt/β-catenin signaling pathways were the key signaling pathways for TCM treatment of DCM. The aim of this study was to summarize and update the signaling pathways for TCM treatment of DCM, to screen potential targets for drug candidates against DCM, and to provide new ideas and more experimental evidence for the clinical use of TCM treatment of DCM.
Dual role of transient receptor potential ankyrin 1 in respiratory and gastrointestinal physiology: From molecular mechanisms to therapeutic targets
Authors: Tekulapally Kavya Reddy , Lee Ji Yeon , Kim Dong Seop , Rahman Md. Mahbubur , Park Chul-Kyu , Kim Yong Ho
Published: July 2024

The transient receptor potential ankyrin 1 (TRPA1) channel plays a pivotal role in the respiratory and gastrointestinal tracts. Within the respiratory system, TRPA1 exhibits diverse distribution patterns across key cell types, including epithelial cells, sensory nerves, and immune cells. Its activation serves as a frontline sensor for inhaled irritants, triggering immediate protective responses, and influencing airway integrity. Furthermore, TRPA1 has been implicated in airway tissue injury, inflammation, and the transition of fibroblasts, thereby posing challenges in conditions, such as severe asthma and fibrosis. In sensory nerves, TRPA1 contributes to nociception, the cough reflex, and bronchoconstriction, highlighting its role in both immediate defense mechanisms and long-term respiratory reflex arcs. In immune cells, TRPA1 may modulate the release of pro-inflammatory mediators, shaping the overall inflammatory landscape. In the gastrointestinal tract, the dynamic expression of TRPA1 in enteric neurons, epithelial cells, and immune cells underscores its multifaceted involvement. It plays a crucial role in gut motility, visceral pain perception, and mucosal defense mechanisms. Dysregulation of TRPA1 in both tracts is associated with various disorders such as asthma, Chronic Obstructive Pulmonary Disease, Irritable Bowel Syndrome, and Inflammatory Bowel Disease. This review emphasizes the potential of TRPA1 as a therapeutic target and discusses the efficacy of TRPA1 antagonists in preclinical studies and their promise for addressing respiratory and gastrointestinal conditions. Understanding the intricate interactions and cross-talk of TRPA1 across different cell types provides insight into its versatile role in maintaining homeostasis in vital physiological systems, offering a foundation for targeted therapeutic interventions.
Knowledge gaps on grape sour rot inferred from a systematic literature review
Authors: Brischetto Chiara , Rossi Vittorio , Fedele Giorgia
Published: July 2024

Sour rot (SR) is one of the major diseases affecting grapevine berries, causing severe yield losses and deterioration of wine quality. SR is caused by an etiologic complex of microorganisms, including yeasts, bacteria, and filamentous fungi. This systematic review focuses on the etiology, epidemiology, and control of SR. A total of 74 papers published between 1986 and 2023 were assessed in this review. Description of disease symptoms was quite consistent across the papers, including oxidation of the grape skin, disaggregation of the internal tissues, and detachment of the rotten berries from the pedicel. The affected bunches are characterized by the smell of acetic acid and ethyl acetate that attracts fruit flies (Drosophila spp.). However, several knowledge gaps and/or inconsistencies were identified with respect to SR etiology, epidemiology, and control. Overall, 146 microorganisms were isolated from the affected berries (44.5% yeasts, 34.3% bacteria, and 21.2% filamentous fungi); however, the selected papers could not definitively clarify which species are primarily involved in the etiology of the disease. A general inconsistency was also observed in the methods used to assess the incidence and severity of SR in vineyards, making inter-study comparisons extremely challenging. Inconsistencies were also found in the methods used for pathogenicity assessment in artificial inoculation studies. Furthermore, gaps were detected in terms of SR epidemiology, with a focus on environmental conditions affecting the disease development. The SR management options are limited, and efficacy trials often result in poor, variable, and inconsistent levels of control, which might be attributed to the lack of knowledge on disease epidemiology. These knowledge gaps and inconsistencies were analyzed in this review to inform future research activities.
Using exploratory graph analysis (EGA) in validating the structure of the Perth alexithymia questionnaire in Iranians with chronic pain
Authors: Sheykhangafshe Farzin Bagheri , Farahani Hojjatollah , Watson Peter
Published: July 2024

Background

Chronic pain’s influence on emotional well-being can be significant. It may evoke feelings of despair, frustration, nervousness, and melancholy in individuals, which often manifest as reactions to enduring pain and disruptions in their daily lives. In this study, we seek to perform Bootstrap Exploratory Graph Analysis (EGA) on the Persian Version of the Perth Alexithymia Questionnaire (PAQ) in a cohort of people with chronic pain.

Methods

The research concentrated on the population of individuals encountering chronic pain within Tehran province from 2022 to 2023. Ultimately, the analysis comprised information from 234 male participants (with a mean age of 30.59, SD = 6.84) and 307 female participants (with a mean age of 30.16, SD = 6.65). After data collection, statistical analysis was conducted using the EGAnet2.0.4 package in R.4.3.2 software.

Results

The outcome of bootstrapped EGA unveiled a two-dimensional configuration of the PAQ comprising Factor 1 denoted as negative difficulty in describing and identifying feelings (N-DDIF) and Factor 2 characterized as general-externally orientated thinking (GEOT), representing robust structural integrity and item consistency (all items have stabilities > 0.70).

Conclusion

These findings endorse the validity of the PAQ, as evidenced by its confirmation in a broader sample using a novel methodology consistent with existing literature on two-factor decentering models.
Online mindfulness-based cognitive therapy for treatment-resistant depression: a parallel-arm randomized controlled feasibility trial
Authors: Rodrigues Michele Ferreira , Quagliato Laiana , Appolinario Jose Carlos , Nardi Antonio E.
Published: July 2024

Introduction

Treatment-resistant depression (TRD) presents a significant challenge, affecting approximately 30% of individuals diagnosed with major depressive disorder and leading to poor treatment responses. Innovations in digital mental health, especially online mindfulness-based cognitive therapy (eMBCT), offer promising avenues for enhancing access to effective mental health care for individuals with TRD in a clinical setting.

Objective

The aim of this study was to examine the feasibility of eMBCT in an individual clinical context to decrease depressive symptoms for TRD.

Methods

Conducted at the Institute of Psychiatry of the Federal University of Rio de Janeiro, Brazil, this parallel-arm, randomized controlled feasibility trial involved outpatients diagnosed with TRD, aged 18 and above. Of the 39 outpatients invited, 28 were randomized into two groups: an intervention group receiving the eMBCT program (n = 15) and a control group (n = 13). The intervention, consisting of an 8-week course, was delivered via live video sessions. Following the assessment period, participants in the control group were offered the eMBCT intervention. Assessments using standardized questionnaires were conducted at the start and end of the study.

Results

Within the eMBCT group, improvements were observed in depression symptoms (Z = −3.423; p = 0.001; effect size r = 0.78), anxiety symptoms (Z = −3.361; p = 0.001; effect size r = 0.77), with no significant changes in the control group. Comparatively, the eMBCT group showed significant reductions in depression symptoms and improvements in clinical global impressions over the control group (BDI2: U = 30.5; p = 0.015; effect size r = 0.47, CGI1: U = 21.0; p = 0.004; effect size r = 0.56).

Conclusion

eMBCT in an individual format combined with medication, appears to be a feasible treatment for TRD, decreasing symptoms of depression. In a future trial the control group may have a manualized intervention.

Clinical trial registration

The Brazilian Clinical Trials Registry: (https://ensaiosclinicos.gov.br/rg/RBR-6zndpbv) and RBR-6zndpbv.
The impact of centralized band purchasing of pharmaceuticals on innovation of Chinese pharmaceutical firms: an empirical study based on double difference models
Authors: Chen Xinqing , Chen Shiyi , Wu Zikun , Wang Su , Chen Yuwen
Published: July 2024

In 2018, China began to gradually promote the pilot policy of centralized band purchasing of medicines. Implementing this policy has resulted in a significant decrease in drug prices. However, there needs to be a clear consensus on the impact and mechanism of action on the innovation of pharmaceutical companies. Therefore. Taking the data of Chinese Shanghai and Shenzhen A-share pharmaceutical listed companies from 2016 to 2022 as a sample, this paper empirically investigates the impact of the centralized banded purchasing policy of drugs on the innovation of pharmaceutical enterprises by using a double difference model and further analyzes the mechanism of its action. The results show that implementing the centralized banded purchasing policy can promote pharmaceutical enterprises’ innovation input and output, which is robust under the parallel trend and placebo tests. Further exploring the impact mechanism of the centralized band purchasing policy on pharmaceutical enterprises’ innovation, it can be found that it promotes innovation inputs through three channels: government subsidies, enterprise profits, and operating income. In addition, the impact of centralized band purchasing on enterprise innovation is heterogeneous in terms of region, enterprise nature, and scale. Therefore, the positive effects of the centralized band purchasing policy on promoting innovation in pharmaceutical enterprises should be fully recognized, and enterprise heterogeneity should be taken into account when implementing the policy. This study provides empirical evidence on the implementation effect of the centralized banded purchasing policy and provides lessons for continuously optimizing the policy to promote the high-quality development of pharmaceutical enterprises.
Knowledge, attitude, and practice of patients with oral diseases toward oral examinations: a cross-sectional survey study
Authors: Wang Wei
Published: July 2024

Background

Properly adhering to oral hygiene and medical care is an important public health issue. Several studies examined the knowledge, attitudes, and practices (KAP) toward oral care in various populations and generally reported relatively sufficient knowledge but unfavorable attitudes and poor practice. However, no previous studies have examined the KAP toward oral examinations among Chinese patients with oral diseases. This study aimed to examine the KAP toward oral examinations among patients with oral diseases in China.

Methods

This cross-sectional study was conducted in patients with oral diseases who visited The Affiliated Stomatological Hospital of Tongji University between December 2023 and February 2024. Data collection and KAP scores assessment were performed using a self-designed questionnaire.

Results

A total of 519 valid questionnaires were included, with 292 females. The mean knowledge, attitude, and practice scores were 6.42 ± 2.47 (possible range: 0–9 points), 35.04 ± 5.68 (possible range: 10–50 points), and 16.22 ± 2.05 (possible range: 4–20 points), respectively, indicating sufficient knowledge, positive attitudes, and proactive practice. Pearson’s correlation analysis showed that knowledge was positively correlated to attitude (r = 0.468, p < 0.001) and practice (OR = 0.416, p < 0.001). Attitude was positively correlated to the practice (r = 0.503, p < 0.001). Moreover, the structural equation model showed that knowledge influenced attitude (estimate = 1.010, p < 0.001) and practice (estimate = 0.169, p < 0.001). Attitude influenced practice (estimate = 0.122, p < 0.001). The frequency of oral examination per year influenced knowledge (estimate = −0.761, p < 0.001) and practice (estimate = −0.515, p < 0.001). Expenses for oral disease per year influenced attitude (estimate = 0.537, p < 0.001).

Conclusion

Patients with oral disease might have sufficient knowledge, positive attitude, and proactive practice toward oral examinations. Specific knowledge items were identified to require improvements.
Prevalence of neurological diseases associated with cervical pain and/or signs of cervical myelopathy in French bulldogs: a retrospective analysis of 105 cases
Authors: Capasso Michele , Canal Sara , Balducci Federica
Published: July 2024

Introduction

French bulldogs can be affected by several neurological diseases, with myelopathies representing the most frequent cause of neurological signs. Studies focusing on the epidemiology of cervical diseases in this breed are lacking. This study aims to describe the prevalence of neurological pathologies responsible for cervical pain and/or signs of cervical myelopathy, assuming that intervertebral disc herniation represents the most common disease; a second aim was to evaluate how often different pathologies coexist in this spinal region in French bulldogs.

Materials and methods

A retrospective analysis of medical records from the database of a single referral center (AniCura I Portoni Rossi Veterinary Hospital—Zola Predosa, Bologna, Italy) was performed, including French bulldogs presented for neck pain and/or neurological deficits consistent with cervical myelopathy. Clinical and imaging data were reviewed and used as inclusion criteria. Based on the number of MRI-diagnosed diseases, the eligible dogs were divided into three groups.

Results

One hundred five French bulldogs met the inclusion criteria. The most commonly diagnosed condition was an intervertebral disc herniation (66.9%), followed by C2 idiopathic hypertrophic ganglioneuritis (15.1%), cervical syringomyelia (11.5%), congenital osseous malformations (1.4%), spinal arachnoid diverticula (1.4%), neoplasms (1.4%), steroid-responsive meningitis-arteritis (0.7%), traumatic vertebral fractures (0.7%), and other (0.7%). For the vast majority of dogs (75/105) a single pathology was diagnosed, with intervertebral disc herniations accounting for 86.7% of cases, involving C3–C4 IVD most commonly. In the remaining 30 dogs, two or three different and concurrent diseases were detected. Among these 30 dogs, intervertebral disc herniations still remained the most diagnosed condition, in combination with C2 idiopathic hypertrophic ganglioneuritis and syringomyelia in 19 and seven cases, respectively.

Conclusion

The results of this study highlight that different pathologies can affect the cervical spinal cord in French bulldogs, with intervertebral disc herniations representing the most frequent condition, as previously described in the veterinary literature. In almost a third of cases, different pathologies can coexist at the cervical level. However, for cases in which different pathologies are present at the same time, it is not always possible to clearly establish their clinical significance.
A New CPX Drum Test to Obtain Sound Pressure Levels of Tyre Noise for Type Approval
Authors: Clar-Garcia David , Campello-Vicente Hector , Campillo-Davo Nuria , Sanchez-Lozano Miguel , Velasco-Sanchez Emilio
Published: July 2024

The primary cause of noise from vehicular traffic while travelling at speeds over 30 km/h is tyre/road interaction. To reduce this noise source, tyre/road sound emissions research has been carried out using different approaches. Most of this research has been centred around track tests, leading to the development of various track and road-based methods for evaluating tyre/road noise emissions. The CPX (Close-Proximity), along with the CPB (Controlled Pass-By), the CB (Coast-By) and the SPB (Statistical Pass-By), methods are the most common ones. Nevertheless, since Reg. (EC) 1222/2009 came into force, only the CB method, defined in Reg. (EC) 117/2007, can be used to obtain tyre/road noise emission type approval values in Europe. However, current track test methods have important limitations, such as the variability of the results depending on the test track or the test vehicle, the repeatability, the influence of environmental variables or, the main aspect, the limitation of the registered magnitude in these tests, which is the sound pressure level. The Alternative Drum test method (A-DR) was developed in 2015 in order to avoid these disadvantages. However, it involves a complex and time-consuming microphone array for each test. With the purpose of improving the A-DR test method, a new methodology based on drum tests, the ISO 11819-2 and the ISO 3744 standards, was developed. This paper describes the new Alternative CPX Drum test method (A-CPX-DR) and validates it by testing several tyres according to the CB, the A-DR and the A-CPX-DR test methods and comparing their results. This research has demonstrated that all three methods have equivalent sound spectra and obtain close equivalent sound pressure levels for type approval of tyres in the EU, while drum tests have shown greater accuracy. For both reasons, the new A-CPX-DR methodology could be used for tyre/road noise emission type approval in a more precise and cheaper way.
Ultrasonic Study of Longitudinal Critically Refracted and Bulk Waves of the Heat-Affected Zone of a Low-Carbon Steel Welded Joint under Fatigue
Authors: Gonchar Alexander , Solovyov Alexander , Klyushnikov Vyacheslav
Published: July 2024

Currently, ultrasonic methods for assessing the fatigue lifetime of various structural materials are being actively developed. Many steel constructions are made by welding. The weld heat-affected zone is the weak point of the construction, as it is most susceptible to destruction. Therefore, it is actually important to search for acoustic parameters that uniquely characterize the structural damage accumulation in the heat-affected zone of a welded joint in order to predict failure. In this work, the specimens were made from the base metal and the welded joint’s heat-affected zone. The specimens were subjected to uniaxial tension–compression under a symmetrical cycle in the region of low-cycle fatigue with control of the strain amplitude. The propagation bulk velocities of longitudinal, shear waves and subsurface longitudinal critically refracted (LCR) waves during cyclic loading were studied. The acoustic birefringence of shear waves was calculated, and a similar parameter was proposed for longitudinal and LCR waves. The dependence of the elastic modulus ratio on the cycle ratio was obtained. It was shown that the acoustic parameters change most intensively in the heat-affected zone. According to the data of the C33/C55 ratio changes measured through the ultrasonic method, a formula for calculating the remaining fatigue life in the heat-affected zone was proposed.
On the Applicability of Kramers–Kronig Dispersion Relations to Guided and Surface Waves
Authors: Krylov Victor V.
Published: July 2024

In unbounded media, the acoustic attenuation as function of frequency is related to the frequency-dependent sound velocity (dispersion) via Kramers–Kronig dispersion relations. These relations are fundamentally important for better understanding of the nature of attenuation and dispersion and as a tool in physical acoustics measurements, where they can be used for control purposes. However, physical acoustic measurements are frequently carried out not in unbounded media but in acoustic waveguides, e.g., inside liquid-filled pipes. Surface acoustic waves are also often used for physical acoustics measurements. In the present work, the applicability of Kramers–Kronig relations to guided and surface waves is investigated using the approach based on the theory of functions of complex variables. It is demonstrated that Kramers–Kronig relations have limited applicability to guided and surface waves. In particular, they are not applicable to waves propagating in waveguides characterised by the possibility of wave energy leakage from the waveguides into the surrounding medium. For waveguides without leakages, e.g., those formed by rigid walls, Kramers–Kronig relations remain valid for both ideal and viscous liquids. Examples of numerical calculations of wave dispersion and attenuation using Kramers–Kronig relations, where applicable, are presented for unbounded media and for waveguides formed by two rigid walls.
Trajectory Design of Potentially Hazardous Asteroid Exploration with Reusable Probes from Cislunar Space
Authors: Peng Chao , Zhang Renyong , Gao Yang
Published: July 2024

This article presents a trajectory design problem concerning the exploration of potentially hazardous near-Earth asteroids (PHAs) with reusable probes from cislunar space. A total of 20 probes, making round trips departing from and returning to a service space station in a lunar distant retrograde orbit, are expected to explore as many PHAs as possible by means of close flyby within a 10-year time window. The trajectory design problem was released in the 12th edition of China’s Trajectory Optimization Competition on 20 August 2022, and a total of 10 sets of trajectory solutions were submitted. As the authors who proposed the competition problem, we present in this article the problem descriptions, trajectory analysis, and design, as well as an impressive trajectory solution in which a total of 105 PHAs are explored. It is concluded that taking advantage of reusable probes from cislunar space is a promising option to efficiently explore large numbers of PHAs.
Robust Trajectory Planning of Gliding-Guided Projectiles with Weak Maneuverability
Authors: Yin Qiulin , Chen Qi , Wang Zhongyuan , Wang Qinghai
Published: July 2024

Due to constraints in launch platforms and cost, the maneuverability of gliding-guided projectiles is limited, necessitating a rational design of their trajectory schemes. To reduce the sensitivity of trajectory schemes to uncertainties while ensuring compatibility between flight schemes and guidance control systems and fully exploiting the control capability of the projectile, a closed-loop robust trajectory planning method is proposed. Models of major uncertain factors and state deviation at the control start point are established. Based on the NIPCE method, the stochastic dynamic model is transformed into a high-dimensional deterministic model with PCE coefficients as state variables, and the uncertainty propagation law is obtained. A PID algorithm is employed to design a tracking guidance law based on position error feedback, and open-loop and closed-loop robust trajectory planning models are established accordingly. The optimal control problem is solved by transforming it into a nonlinear programming problem using the direct shooting method. Our simulation results indicate that the NIPCE method can significantly improve the computational efficiency of uncertainty propagation while ensuring accuracy; compared with parallel MCS, the computation time is reduced by 96.8%. Open-loop robust planning can effectively mitigate the sensitivity of gliding trajectories to uncertainties (the standard deviations of terminal altitude and lateral deviations are reduced by 23.6% and 35.3%, respectively, compared to deterministic planning) but cannot completely eliminate terminal dispersion. Closed-loop robust planning effectively improves control effort consumption on the basis of open-loop planning.
Enhanced Fuzzy-Based Super-Twisting Sliding-Mode Control System for the Cessna Citation X Lateral Motion
Authors: Hosseini Seyed Mohammad , Bematol Ilona , Ghazi Georges , Botez Ruxandra Mihaela
Published: July 2024

A novel combination of three control systems is presented in this paper: an adaptive control system, a type-two fuzzy logic system, and a super-twisting sliding mode control (STSMC) system. This combination was developed at the Laboratory of Applied Research in Active Controls, Avionics and AeroServoElasticity (LARCASE). This controller incorporates two methods to calculate the gains of the switching term in the STSMC utilizing the particle swarm optimization algorithm: (1) adaptive gains and (2) optimized gains. This methodology was applied to a nonlinear model of the Cessna Citation X business jet aircraft generated by the simulation platform developed at the LARCASE in Simulink/MATLAB (R2022b) for aircraft lateral motion. The platform was validated with flight data obtained from a Level-D research aircraft flight simulator manufactured by the CAE (Montreal, Canada). Level D denotes the highest qualification that the FAA issues for research flight simulators. The performances of controllers were evaluated using the turbulence generated by the Dryden model. The simulation results show that this controller can address both turbulence and existing uncertainties. Finally, the controller was validated for 925 flight conditions over the whole flight envelope for a single configuration using both adaptive and optimized gains in switching terms of the STSMC.
Estimating Leaf Chlorophyll Content of Winter Wheat from UAV Multispectral Images Using Machine Learning Algorithms under Different Species, Growth Stages, and Nitrogen Stress Conditions
Authors: Zhang Liyuan , Wang Aichen , Zhang Huiyue , Zhu Qingzhen , Zhang Huihui , Sun Weihong , Niu Yaxiao
Published: July 2024

The rapid and accurate estimation of leaf chlorophyll content (LCC), an important indicator of crop photosynthetic capacity and nutritional status, is of great significance for precise nitrogen fertilization management. To explore the existence of a versatile regression model that can be successfully used to estimate the LCC for different varieties under different growth stages and nitrogen stress conditions, a study was conducted in 2023 across the growing season for winter wheat with five species and five nitrogen application levels. Two machine learning regression algorithms, support vector machine (SVM) and random forest (RF), were used to establish the bridge between UAV-derived multispectral vegetation indices and ground truth LCC (relative chlorophyll content, SPAD), taking the multivariate linear regression (MLR) algorithm as a reference. The results show that the visible atmospherically resistant index, vegetative index, and normalized difference vegetation index had the highest correlation with ground truth LCC, with a Pearson’s correlation coefficient of 0.95. All three regression algorithms (MLR, RF, and SVM) performed well on the training dataset (R2: 0.932–0.944, RMSE: 3.96 to 4.37), but performed differently on validation datasets with different growth stages, species, and nitrogen application levels. Compared to winter wheat species and nitrogen application levels, the growth stages had the greatest influence on the generalization ability of LCC estimation models, especially for the dough stage. At the dough stage, compared to MLR and RF, SVM performed best, with R2 increasing by 0.27 and 0.10, respectively, and RMSE decreasing by 1.13 and 0.46, respectively. Overall, this study demonstrated that the combination of UAV-derived multispectral VIs and the SVM regression algorithm could be successfully applied to map the LCC of winter wheat for different species, growth stages, and nitrogen stress conditions. Ultimately, this research is significant as it shows the successful application of UAV data for mapping the LCC of winter wheat across diverse conditions, offering valuable insights for precision nitrogen fertilization management.
Sorption Drying of Wheat Seeds Using Kieserite as a Solid Desiccant
Authors: Fomenko Elena V. , Anshits Natalia N. , Shabanov Vasily F. , Anshits Alexander G.
Published: July 2024

The moisture content (MC) of wheat seeds must be reduced before storage using appropriate dehydration processes. Desiccant drying is a promising alternative to conventional drying methods because it improves seed quality while providing overall energy efficiency. This study explores the sorption drying of wheat seeds using granulated kieserite MgSO4·H2O as a solid desiccant, which has a high water capacity and is regenerated at low temperatures <100 °C. Desiccant characterization was conducted using SEM-EDS, XRD, DSC-TG, and particle size analysis. Wheat seeds mixed directly with kieserite in various mass ratios were dried under uniform stirring and controlled temperature conditions. A 240-min drying time was required to reduce the initial MC of wheat from 21.5% to 15.1% at a desiccant-to-grain ratio of 1:1. After 360 min, a final MC of 14.4% was achieved. The germination energy and seed capacity after sorption drying were 91 ± 1% and 97 ± 2%, respectively. Due to the available water capacity of kieserite, several batches of seeds can be dried without intermediate desiccant regeneration. This study is useful for developing low-cost, non-thermal, and sustainable drying technology for various agricultural products.
Growth Monitoring of Greenhouse Tomatoes Based on Context Recognition
Authors: Rahman Fisilmi Azizah , Takanayagi Miho , Eguchi Taiga , Yeoh Wen Liang , Yamaguchi Nobuhiko , Okumura Hiroshi , Tanaka Munehiro , Inaba Shigeki , Fukuda Osamu
Published: July 2024

To alleviate social problems in agriculture such as aging and labor force shortages, automatic growth monitoring based on image measurement has been introduced to tomato cultivation in greenhouses. The overlap of leaves and fruits makes precise observations challenging. In this study, we applied context recognition to tomato growth monitoring by using a Bayesian network. The proposed method combines image recognition using convolutional networks and context recognition using Bayesian networks. It enables not only the recognition of individual tomatoes but also the evaluation of tomato plants. An accurate number of tomatoes and the condition of the stocks can be estimated based on the number of ripe and unripened tomatoes in addition to their density information. The verification experiments clarified that a more accurate number of tomatoes could be estimated than with simple tomato detection, and the stock states could also be evaluated correctly. Compared to conventional methods, the method used in this study has improved tomato decision accuracy by 23%.
Comparative Antimicrobial Resistance and Prevalence of Methicillin Resistance in Coagulase-Positive Staphylococci from Conventional and Organic Dairy Farms in South Korea
Authors: Neri Therese Ariane N. , Park Hyunjung , Kang Sujin , Baek Seung Hee , Nam In Sik
Published: July 2024

Bovine mastitis (BM) has caused huge economic and financial losses in the dairy industry worldwide, with Staphylococcus aureus as one of its major pathogens. BM treatment still relies on antibiotics and its extensive use often generates methicillin-resistant S. aureus (MRSA) and mupirocin-resistant S. aureus (MuRSA). This study compared the antimicrobial resistance trend in coagulase-positive Stapholococci (CoPS) isolated from BM milk in conventional and organic dairy farms and checked prevalence of MRSA and MuRSA. A total of 163 presumptive Staphylococci were isolated, wherein 11 out of 74 from 4 conventional farms (CF1, CF2, CF3, CF4) and 17 out of 89 from 3 organic farms (OF1, OF2, OF3) exhibited coagulase activity. Multiplex-PCR amplification confirmed at least one coagulase-positive isolate from CF1, CF2, CF3, CF4, and OF1 as S. aureus, denoted by the presence of the nuc gene. Three isolates from CF2 contained the mecA gene, indicating MRSA prevalence, while the MuRSA gene marker, mupA, was not detected in any of the isolates. Antimicrobial testing showed that conventional farm isolates were more resistant to antibiotics, especially ampicillin and tetracycline. This suggests a risk of developing multidrug resistance in dairy farms if antibiotic use is not properly and strictly monitored and regulated.