bluuebunny/crossref_metadata_2025 huggingface dataset:
Utilization of mental health services and associated factors among residents of southern Ethiopia; a community based cross-sectional study
Authors: Alamirew Birhanu , Darge Beniam D. , Terefe Bezabih , Gebremeskel Feleke
Published: February 2025

Background

In 2019, nearly one billion individuals worldwide were estimated to be living with some form of mental illness. This staggering figure underscores not only the widespread prevalence of mental health issues but also their significant negative impact. Despite the critical impact of mental health problems, there is a substantial gap in treatment at the global scale. Particularly in rural Ethiopia, there is a notable lack of data regarding the use of mental health services by community residents. This study was conducted with the aim of evaluating the utilization of mental health services and identifying factors that influence access to these services among the population of rural southern Ethiopia.

Methods

A community-based cross-sectional study was conducted on randomly selected adults in southern Ethiopia. A semi-structured questionnaire assessing the sociodemographic status of the participants was used. Depression and anxiety were measured with the Patient Health Questionnaire (PHQ-9) and the General Anxiety Disorders Scale (GAD-7), respectively. The data were then analyzed using StataMP version 18. Statistical tests such as frequency, percentage, mean, bivariate logistic regression, and multivariate analysis were performed. P values and confidence intervals were used to determine statistical significance.

Results

A total of 971 participants were enrolled in this study, and the mean age was 34.9 (± 11) years. A minority of the participants (152 [15.5%]) sought some form of help for mental health-related reasons. Only 24 (2.5%) of the participants used biomedical mental health services. The perceived need for any mental health service was 96 (9.9%). Thirty-three percent of the respondents with mild to moderate depression and 37.1% of those with severe depression sought care for their concerns. Similarly, 37% and 35% of individuals with mild to moderate anxiety and severe anxiety, respectively, utilized mental health services. A higher monthly income, psychoactive substance use, perceived need, and symptoms of depression and anxiety were significantly associated with mental health service utilization.

Conclusion

Our study revealed a significantly low level of utilization of mental health services among the residents of rural southern Ethiopia. More efforts to address the treatment gap for mental health problems in the country are needed.
Emergence of population heterogeneity in Klebsiella pneumoniae with a blaOXA-232-harboring plasmid: carbapenem resistance, virulence, and fitness
Authors: Cho Yun Young , Kim Sun Ju , Ko Kwan Soo
Published: February 2025

Background

This study aimed to investigate the population heterogeneity on carbapenem susceptibility in Klebsiella pneumoniae strains that acquired a bla
OXA-232-bearing ColE-type plasmid.

Methods

A bla
OXA-232-bearing plasmid was electroporated into two carbapenem-susceptible K. pneumoniae strains. High- and low-carbapenem-resistant subpopulations were identified and isolated using patch plating. The strains were subsequently subcultured in antibiotic-free media, yielding two distinct populations: a stable, high-level carbapenem-resistant strains and a heterogeneous strains. Antibiotic susceptibility tests, time-killing assays, and population profiles were conducted, along with a competition assay was performed and the growth curve analysis. To assess virulence, we performed human serum resistance and Galleria mellonella infection assays, and measured the expression of virulence genes using qRT-PCR. Additionally, whole genome sequencing was carried out for further anaysis.

Results

Introduction of pOXA-232 into carbapenem-susceptible K. pneumoniae strains resulted in two isogenic transformants with distinct resistance profiles: an unstable, high-level carbapenem-resistant (HCR), and highly virulent subpopulation; and a stable, low-level carbapenem-resistant (LCR), and low-virulence subpopulation. Whole genome and expression analyses revealed dysfunctionality of ompK36 in HCR subpopulations. Subculturing of HCR led to the re-emergence of heterogeneous populations with variations in carbapenem resistance and an additional compensatory mutation of 9,000 bp deletion in the genome. Thus, stable HCR strains featuring both mutations in ompK36 and compensatory mutations developed.

Conclusion

This study demonstrated that underlying heterogeneity can promote the emergence of stable, high-level antibiotic resistance, even with the introduction of a plasmid carrying a low-level antibiotic resistance gene, such as bla
OXA-232. This highlights the critical need to closely monitor bacterial population dynamics.
Lower limb pointing to assess intersegmental dynamics after incomplete spinal cord injury and the associated role of proprioceptive impairments
Authors: Malik Raza N. , Marigold Daniel S. , Chow Mason , Eginyan Gevorg , Lam Tania
Published: February 2025

Background

Disorders in the recovery of gait strategies in individuals with incomplete spinal cord injury (SCI) suggest difficulties in controlling lower limb intersegmental dynamics, which could relate to proprioceptive impairments. To probe discrete aspects of lower limb interjoint coordination, we present here a novel protocol to assess lower limb motor strategies and evaluate the influence of proprioceptive impairments following SCI.

Methods

Twelve able-bodied controls and 16 participants with SCI performed lower limb pointing to three targets that involved combined hip and knee flexion, or hip or knee flexion only while standing, with either full or obstructed visual feedback. We quantified lower limb proprioceptive sense in individuals with SCI using a robotic gait device. We used motion analysis to determine lower limb joint angles and foot trajectory, computed inverse dynamics to quantify joint and intersegmental dynamics, and derived muscle torque as an indicator of the motor strategies produced to control the motion to each target. We used linear mixed-effects models to assess differences between the control and SCI groups on end-point performance and muscle torque, and to assess the relationship of muscle torque with end-point performance and proprioceptive sense.

Results

Groups differed in motor strategies, but not end-point performance, when pointing to all three targets. Compared to controls, the SCI group had difficulty controlling knee muscle torque when performing the hip-flexion-only target (p = 0.008) or when flexing the hip and knee simultaneously (p = 0.0004). To complete the knee-flexion-only target, the SCI group had difficulties generating the required hip extensor muscle torque to maintain the thigh in neutral (p = 0.0001). These altered motor strategies in individuals with SCI were associated with proprioceptive impairments and end-point performance.

Conclusion

This novel lower limb pointing task can identify disordered motor strategies in individuals with SCI, especially at the knee, and are associated with proprioceptive impairment. Variations of this paradigm can be employed to further understand differences in motor strategies between controls and individuals with SCI, and the impact of proprioceptive deficits.
Engineered oncolytic virus coated with anti-PD-1 and alendronate for ameliorating intratumoral T cell hypofunction
Authors: Zhu Yufu , Zhang Xuefeng , Jin Jiaqi , Wang Xiaoqian , Liu Yang , Gao Jian , Hang Diancheng , Fang Lin , Zhang Hengzhu , Liu Hongmei
Published: February 2025

Background

Glioblastoma is a highly aggressive and devastating primary brain tumor that is resistant to conventional therapies. Oncolytic viruses represent a promising therapeutic approach for glioblastoma by selectively lysing tumor cells and eliciting an anti-tumor immune response. However, the clinical efficacy of oncolytic viruses is often hindered by challenges such as short persistence, host antiviral immune responses, and T cell dysfunction.

Methods

We have developed a novel therapeutic strategy by “dressing” oncolytic viruses with anti-PD-1 antibodies and alendronate (PD-1/Al@OV) to prevent premature clearance of the oncolytic viruses and enhance T cell function, thereby improving immunotherapy outcomes against glioma.

Results

We found that in the high reactive oxygen species environment of the tumor, PD-1/Al@OV disassembled to release oncolytic viruses, anti-PD-1, and alendronate. The released anti-PD-1 blocked the PD-1/PD-L1 pathway, activating T cells; the alendronate eliminated tumor-associated macrophages, increasing the concentration of oncolytic viruses; and the oncolytic viruses directly lysed cancer cells, enhancing intratumoral T cell infiltration.

Conclusion

This approach effectively improved the immunosuppressive microenvironment of glioblastoma and achieved a robust anti-tumor effect. Consequently, this study presents a novel strategy for immune combination therapy and the improvement of the glioblastoma immune microenvironment, thereby offering new prospects for the clinical application of oncolytic viruses.
Association among parents’ stress recovery experiences, parenting practices, and children’s behavioral problems: a cross-sectional study
Authors: Hosokawa Rikuya , Katura Toshiki
Published: February 2025

Background

Parents can experience much stress from parenting, work, and household responsibilities. Parents’ stress recovery experiences, or their lack thereof, can affect parenting practices and influence children’s behavioral problems, which may thereby lead to difficulties for children later in life. Therefore, the relationships among these three factors deserve consideration. This study tested a model of the mediating role of parenting practices in the relationship between parents’ stress recovery experiences and children’s behavioral problems.

Methods

Parents (N = 1,112) of 14-year-old children in the third year of junior high school in Japan completed a questionnaire, yielding 583 valid responses. To accurately determine the relationship among parents’ stress recovery experiences, parenting practices, and children’s behavioral problems, parents of children diagnosed with developmental disabilities and parents who did not respond to the required items in the questionnaire were excluded from the analysis. As a result, 536 of the 583 (89.0%) parents met the inclusion criteria. We conducted a path analysis, following the hypothesis that parents’ stress recovery experiences, via their parenting practices, are associated with children’s behavioral problems.

Results

The path analysis results indicated that parents’ stress recovery experiences of relaxation and mastery were positively associated with positive nurturing attitudes, whereas mastery and control were negatively associated with negative nurturing attitudes. Furthermore, positive nurturing attitudes were negatively associated with externalizing and internalizing problem behaviors, whereas negative nurturing attitudes were positively associated with externalizing and internalizing problem behaviors. In other words, the hypothesis that parents’ stress recovery experiences of relaxation, mastery, and control reduce children’s behavioral problems via promoting nurturing parental attitudes was supported.

Conclusions

The results indicate that the higher the level of parents’ stress recovery experiences, the lower the level of reported children’s behavioral problems. Parents’ stress recovery experiences correlated with parenting practices, which partially mediated the relationship of the parents’ stress recovery with children’s behavioral problems. The suggestion is that increasing parents’ stress recovery experiences, improving parenting practices and related behaviors, and strengthening the parent–child relationship are important measures that can be mutually beneficial for parents, children, and the overall family relationship.
Retinal ganglion cell vulnerability to pathogenic tau in Alzheimer’s disease
Authors: Davis Miyah R. , Robinson Edward , Koronyo Yosef , Salobrar-Garcia Elena , Rentsendorj Altan , Gaire Bhakta P. , Mirzaei Nazanin , Kayed Rakez , Sadun Alfredo A. , Ljubimov Alexander V. , Schneider Lon S. , Hawes Debra , Black Keith L. , Fuchs Dieu-Trang , Koronyo-Hamaoui Maya
Published: February 2025

Pathological tau isoforms, including hyperphosphorylated tau at serine 396 (pS396-tau) and tau oligomers (Oligo-tau), are elevated in the retinas of patients with mild cognitive impairment (MCI) due to Alzheimer’s disease (AD) and AD dementia. These patients exhibit significant retinal ganglion cell (RGC) loss, however the presence of tau isoforms in RGCs and their impact on RGC integrity, particularly in early AD, have not been studied. Here, we analyzed retinal superior temporal cross-sections from 25 MCI or AD patients and 16 age- and sex-matched cognitively normal controls. Using the RGC marker ribonucleic acid binding protein with multiple splicing (RBPMS) and Nissl staining, we found a 46–56% reduction in RBPMS+ RGCs and Nissl+ neurons in the ganglion cell layer (GCL) of MCI and AD retinas (P < 0.05–0.001). RGC loss was accompanied by soma hypertrophy (10–50% enlargement, P < 0.05–0.0001), nuclear displacement, apoptosis (30–50% increase, P < 0.05–0.01), and prominent expression of granulovacuolar degeneration (GVD) bodies and GVD-necroptotic markers. Both pS396-tau and Oligo-tau were identified in RGCs, including in hypertrophic cells. PS396-tau+ and Oligo-tau+ RGC counts were significantly increased by 2.1–3.5-fold in MCI and AD retinas versus control retinas (P < 0.05–0.0001). Tauopathy-laden RGCs strongly inter-correlated (r

P
=0.85, P < 0.0001) and retinal tauopathy associated with RGC reduction (r

P
=-0.40–(-0.64), P < 0.05–0.01). Their abundance correlated with brain pathology and cognitive deficits, with higher tauopathy-laden RGCs in patients with Braak stages (V–VI), clinical dementia ratings (CDR = 3), and mini-mental state examination (MMSE ≤   26) scores. PS396-tau+ RGCs in the central and mid-periphery showed the closest associations with disease status, while Oligo-tau+ RGCs in the mid-periphery exhibited the strongest correlations with brain pathology (NFTs, Braak stages, ABC scores; r

S
=0.78–0.81, P < 0.001–0.0001) and cognitive decline (MMSE; r

S
=-0.79, P = 0.0019). Overall, these findings identify a link between pathogenic tau in RGCs and RGC degeneration in AD, involving apoptotic and GVD-necroptotic cell death pathways. Future research should validate these results in larger and more diverse cohorts and develop RGC tauopathy as a potential noninvasive biomarker for early detection and monitoring of AD progression.
Spatiotemporal dynamics reveal high turnover and contrasting assembly processes in fungal communities across contiguous habitats of tropical forests
Authors: Lin Chieh-Ping , Lin Yu-Fei , Liu Yu-Ching , Lu Mei-Yeh Jade , Ke Huei-Mien , Tsai Isheng Jason
Published: February 2025

Background

The variation in fungal community composition within a single habitat space has been extensively studied in forest ecosystems. However, the spatial and temporal distribution of fungi across contiguous habitats, particularly at a local scale and in tropical regions, remains underexplored. In this study, we examined the fungal community composition across multiple habitats proximal to each other over two seasons in seven Fagaceae species in Taiwanese broadleaf forests. We tested how local spatial scale and habitat influence community assembly.

Results

Using a metabarcoding approach, we sequenced ITS2 regions from 864 samples collected from four distinct habitats—leaves, twigs, litter, and soil. We identified 11,600 fungal amplicon sequence variants (ASVs), with community composition differing significantly between habitats proximal to each other. Generalized dissimilarity modeling (GDM) revealed that spatial distance, interacting with precipitation, was the strongest predictor of fungal turnover, particularly in the phyllosphere. Normalized Stochasticity Ratio (NST) analyses further highlighted contrasting assembly processes, with deterministic influences dominating in the phyllosphere habitat, while stochasticity prevailed in soil and litter. Random forest analysis accurately classified habitats based on ASVs’ relative abundances, with strong predictors were mostly habitat-specific ASVs prevalent in soil. Misclassified samples were due to secondary contact of fungi between adjacent habitats. Co-occurrence network analysis revealed more complex and deterministic networks in leaf and twig habitats, while soil was driven by stochastic processes and contained most habitat-specific ASVs. A Cladosporium sp. emerged as a keystone species, maintaining network stability across forests.

Conclusion

This study reveals how local spatial variation and habitat shape distinct fungal communities in tropical forests, with deterministic processes dominating in some habitats and stochasticity playing a key role in others. We show extremely high turnover in fungal community are present over very short distances and that local fungal taxa are strong habitat predictors. These findings highlight the importance of studying coexisting habitats to gain a deeper understanding of fungal biogeography and ecosystem function.
Current status of forensic anthropology in the Middle East and North Africa (MENA) region
Authors: Mansour Cindy , Márquez-Grant Nicholas , Benito Sánchez María
Published: February 2025

Background

Forensic anthropology has evolved significantly, from its foundations in the nineteenth century to its formal establishment in the twentieth century and in particular with modern advancements from the 1970s onward. Its role in human rights investigations during the 1980s in Latin America and the 1990s in the Balkans, exemplifies its global impact. However, the practice and application of forensic anthropology in the Middle East and North Africa (MENA) region remain underexplored. This study assesses the current status of forensic anthropology in this region through a brief literature review and online interviews with academics and practitioners in forensic anthropology or closely related disciplines. The interviews addressed the medico-legal system structure, forensic science capabilities, training efforts, practitioner availability, case types (medico-legal and humanitarian), and resources like radiological imaging.

Results

The study revealed that forensic anthropology is largely underutilized in the MENA region’s medico-legal death investigation systems. Factors such as limited human capital, lack of discipline awareness, varying legal and procedural systems, and insufficient academic infrastructure hinder its integration. Challenges include political instability, safety concerns for practitioners, and inadequate resources. The study highlights ongoing efforts by practitioners to improve the field through theoretical and practical training, capacity building, and resource development.

Conclusion

The findings underscore the need for strategic investments to strengthen forensic anthropology in the MENA region. Recommendations include enhancing education and training, fostering interdisciplinary collaboration, disseminating scientific knowledge, increasing access to resources, and revising medico-legal frameworks. These measures can bridge existing gaps and advance forensic anthropology’s role in medico-legal and humanitarian contexts.
Vagus nerve stimulation combined with nerve rehabilitation therapy for upper limb paralysis after hemorrhagic stroke: a stroke-related epilepsy case
Authors: Xia Chunsheng , Xu Peng , Wang Lanlan , Zhang Dong , Qi Yinbao , Wu Ming , Qian Ruobing
Published: February 2025

Background

Hemorrhagic stroke has a high incidence, often leaving patients with significant complications such as limb mobility disorders after treatment. Traditional treatment methods for stroke patients mainly include limb function exercises and hyperbaric oxygen therapy, which have shown effective results. In recent years, there have been reports utilizing vagus nerve stimulation (VNS) to treat limb paralysis in ischemic stroke patients, achieving encouraging outcomes. However, there are rare related reports on hemorrhagic stroke.

Case presentation

This report presents a case of a patient who developed left upper limb hemiplegia and recurrent seizures after a hemorrhagic stroke. The patient showed a poor response to standard anti-epileptic treatment and was diagnosed with stroke-related epilepsy. To manage the recurrent seizures, VNS was performed. After the device was activated, the patient reported a significant reduction in abnormal muscle tone and increased mobility impairment in the affected upper limb. Parameters were adjusted, and intermittent stroke electrical stimulation was combined with upper limb rehabilitation exercises. After three months of active treatment, the patient’s seizures were well controlled, and there was significant improvement in upper limb function.

Conclusions

VNS has potential in the rehabilitative treatment of stroke patients with upper limb dysfunction. It is hoped that more patients will benefit from this advanced treatment method in the future, regaining their health and vitality. Additionally, future research needs to further explore the mechanisms and methods of brain remodeling to provide theoretical support and more effective treatment options for stroke patient rehabilitation.
Stability of Anti-Drug Antibodies Against Antibody Therapeutics
Authors: Sirohi Parul , Hainline Kelly , Bullock Heather , Wang Sihe , Konrad Robert J. , Wen Yi
Published: February 2025

Biotherapeutics have the potential to elicit unwanted immune responses that can lead to the production of anti-drug antibodies (ADA). It is critical that ADA responses are detected, characterized, and monitored to understand the safety and efficacy of a drug. ADA samples must remain stable in long- and short-term storage conditions to ensure reliable analysis. Whereas the stability of anti-vaccine antibodies has been well-studied, there are few reports examining the stability of anti-therapeutic antibodies using clinical samples. In this study, ADA samples from four clinical trials of antibody therapeutics were found to be stable after long-term storage (1–10 years) at -80°C and short-term storage (24 h to two weeks) at 4°C, 22°C, and 37°C. In addition, samples were stable after 16 freeze/thaw cycles. The results demonstrate the stability of ADA in clinical samples under various conditions. Consequently, the results observed herein suggest that the routine assessment of ADA sample stability may not be warranted.

Graphical Abstract
Review of the role of dietary fiber in the prevention of colorectal cancer
Authors: Łęcka Martyna , Paruzel Marcel , Załóg Adam , Pietryszak Edyta , Żak Katarzyna , Nowak Maria , Wojtasik Monika , Szołtek Paulina , Pardela Rafał , Kulej Wojciech
Published: February 2025

Colorectal cancer (CRC) is one of the most prevalent types of cancers, whose etiology has been highly related to diet. High intake of dietary fiber has been found to favor the reduction of CRC risk, as deduced from a lot of studies. The aim of this review is to explore the role of fiber in CRC chemoprevention and to provide insight into the mechanisms underlying the reduced risk of this neoplasia associated with the consumption of fiber. In this work, the authors describe the state of the literature regarding the association between intake and CRC risk through presenting data from epidemiological investigations, clinical intervention studies, and molecular mechanisms. Data were assembled from studies like the European Prospective Investigation into Cancer and Nutrition (EPIC) and fiber supplementation studies to assess the role of dietary fiber on CRC. The analysis indicated that dietary fiber intake, particularly from fruit, vegetable, and whole grain sources, is inversely related to CRC risk. Most studies, such as meta-analyses and large cohort studies, presented the significantly reduced CRC incidence of the highest intake of fiber. The favorable benefits of fiber are attributed to various modes of mechanisms, including the modulation of gut microbiota, bile acid metabolism, and a reduction in oxidative stress. Dietary fiber might play a vital role in this regard and lower the risk of CRC through its effects on different biological pathways. Promotion of dietary fiber intake could be a key strategy for the prevention of CRC, especially via a balanced diet rich in fruits, vegetables, and whole grains. Public health initiatives that aim at increasing fiber intake should start early in life to prevent not only metabolic but also neoplastic diseases more influenced by dietary factors. However, fiber supplementation appears promising, although further large-scale studies will be required to establish its definite role in CRC chemoprevention.
Developing a Phylogeny Based Machine Learning Algorithm for Metagenomics
Authors:   , Rong Ruichen
Published: February 2025

Metagenomics is the study of the totality of the complete genetic elements discovered from a defined environment. Different from traditional microbiology study, which only analyzes a small percent of microbes that could survive in laboratory, metagenomics allows researchers to get entire genetic information from all the samples in the communities. So metagenomics enables understanding of the target environments and the hidden relationships between bacteria and diseases. In order to efficiently analyze the metagenomics data, cutting-edge technologies for analyzing the relationships among microbes and communities are required. To overcome the challenges brought by rapid growth in metagenomics datasets, advances in novel methodologies for interpreting metagenomics data are clearly needed. The first two chapters of this dissertation summarize and compare the widely-used methods in metagenomics and integrate these methods into pipelines. Properly analyzing metagenomics data requires a variety of bioinformatcis and statistical approaches to deal with different situations. The raw reads from sequencing centers need to be processed and denoised by several steps and then be further interpreted by ecological and statistical analysis. So understanding these algorithms and combining different approaches could potentially reduce the influence of noises and biases at different steps. And an efficient and accurate pipeline is important to robustly decipher the differences and functionality of bacteria in communities. Traditional statistical analysis and machine learning algorithms have their limitations on analyzing metagenomics data. Thus, rest three chapters describe a new phylogeny based machine learning and feature selection algorithm to overcome these problems. The new method outperforms traditional algorithms and can provide more robust candidate microbes for further analysis. With the frowing sample size, deep neural network could potentially describe more complicated characteristic of data and thus improve model accuracy. So a deep learning framework is designed on top of the shallow learning algorithm stated above in order to further improve the prediction and selection accuracy. The present dissertation work provides a powerful tool that utilizes machine learning techniques to identify signature bacteria and key information from huge amount of metagenomics data.
The Influence of Chinese Instruments on the Violin: A Practice Guide of Three Violin Techniques
Authors:   , Gao Jie
Published: February 2025

Contemporary professional violinists face constant exposure to multicultural compositions. For best results, they should be able to understand, capture, and express the subtleties of different styles. The violin and its repertoire spread to China through European missionaries during the late seventeenth century and continued to be developed by Chinese scientists and musicians who studied abroad. During the twentieth and twenty-first centuries, Chinese composers wrote many violin pieces inspired by the unique sounds of Chinese instruments. Additionally, Chinese music scholars wrote numerous essays to discuss the new Chinese style. However, much of this research has been focused on the composers and the structures of the compositions rather than on the details of violin techniques necessary to play the repertoire. The techniques in Chinese violin compositions are unique and are influenced by the traditional instruments including string, wind, and percussion instruments. Furthermore, the style of such compositions is affected by the elements of Chinese culture, such as the language, the elite society and its poetic tradition, and historical legends and events. This dissertation provides examples of Chinese violin repertoire which demonstrate the principles of three main violin techniques in the Chinese style: slides, chords, and pizzicati. In order to help professional violinists better perform Chinese violin compositions, the dissertation also includes a number of exercises covering each technique above.
Trajectories of Treatment Change among Patients with Major Depressive Disorder: Predictors and Associations with Outcome
Authors:   , Kilmer Jared N.
Published: February 2025

Previous research has revealed heterogeneity in outcome trajectories among individuals seeking psychotherapy. However, questions remain as to the number, nature, and predictors of these trajectories. Therefore, the present study had three aims: 1) to identify heterogeneous latent groups among treatment trajectories of 212 clients with major depressive disorder (MDD) seeking psychotherapy at a community mental health training clinic; 2) to identify significant associations between clinical and demographic variables and group membership; and 3) to identify correlations between trajectory shape and positive treatment outcome. Prior to treatment, participants provided demographic information and completed symptom severity ratings. Once in treatment, participants completed a self-report of distress via the Outcome Questionnaire (OQ-45) at every session. Growth mixture modeling was utilized to identify distinct patient subgroups based on outcome trajectories among the sample. Three distinct latent classes of treatment trajectory were identified, providing evidence of heterogeneity in treatment trajectories among individuals with MDD. Baseline distress, pre-treatment work problems, and sleep difficulties were found to be predictive of an individual's membership in a specific trajectory group. Finally, specific shapes of change, namely early response and sudden gains, were associated with positive treatment outcome. Findings from this study can be used to identify patients at risk for treatment failure, allowing clinicians to intervene earlier to enhance mid-treatment feedback and prognosis.
Cultural Humility, Religion, and Health in Lesbian, Gay, and Bisexual (LGB) Populations
Authors:   , Mosher David K.
Published: February 2025

The purpose of this study was to explore the religion – health link in a sample of adults and undergraduate students (N = 555) that identified as lesbian, gay, or bisexual (LGB), and to explore how perceptions of cultural humility of religious individuals and groups toward LGB individuals affect the relationship between religion and health. First, I found religious commitment among LGB individuals was positively correlated with satisfaction in life, but it was negatively correlated with physical health. Second, I found that cultural humility moderated the relationship between religious commitment and satisfaction in life for LGB individuals involved in a religious community. The lowest levels of satisfaction with life were found for individuals with low religious commitment and perceived the cultural humility of their religious community to be low. However, cultural humility did not moderate the relationship between religious commitment and mental and physical health outcomes. Third, I found cultural humility did not moderate the relationship between religious commitment and minority stress (i.e., internalized homophobia). Fourth, I found that cultural humility was a significant positive predictor of motivations to forgive a hurt caused by a religious individual. I conclude by discussing limitations, areas for future research, and implications for counseling.
Transformative Learning Theory as a Basis for Identifying Barriers to Faculty Confidence in Online Instruction
Authors:   , Allen Julia Elizabeth
Published: February 2025

This study applied the stages of transformative learning to faculty perceptions and application of best practices to online learning. Research questions included: Can transformative learning theory constructs be used to identify potential barriers in faculty development and delivery of online instruction?; How does the stage of transformative learning of online faculty relate to their perceptions about online learning and their application of best practices?; Is there a correlation between stage of transformative learning and the amount of experience with online instruction a faculty member has? Principal component analysis and cluster analysis support a four-component solution. The four constructs equate to Mezirow's four stages of learning: transforming frames of reference through critical reflection of assumptions, validating contested beliefs through discourse, taking action on one's reflective insight, and critically assessing it. Multiple regression analyses were run to predict faculty perceptions on the identified components. Three of these were statistically significant based on years of experience teaching online, the number of professional development workshops taken on online teaching, or both. While the instrument appears to be a valid measurement of transformation of frame of reference, examination of previously contested beliefs, and critical assessment of action, further efforts will be needed before this is a fully validated instrument.
Relationship between Adolescents' Perceptions of Parental Physical Activity and Their Own Well-Being
Authors:   , Yeatts Paul E.
Published: February 2025

The purpose of this study was to examine the relationship between adolescents' perceptions of their parents' encouragement to lose weight and physical activity habits (i.e., exercise habits and sedentary behaviors) and their own physical health-related fitness (HRF) and psychological well-being. Results of structural equation modeling revealed both similarities and differences between boys and girls. Specifically, parental sedentary behavior and encouragement to lose weight related to girls HRF. Among boys, only encouragement to lose weight related to HRF. When inspecting the relationship between HRF and psychological well-being, HRF related to body satisfaction, but not depressive symptoms, among both boys and girls. Results of this study highlight the complex influence that parental perceptions have during adolescent development.
Teacher Self-Efficacy for STEM Talent Development
Authors:   , Margot Kelly C.
Published: February 2025

In order to implement more science, technology, engineering, and mathematics (STEM) into K12 classrooms, it is important to find out whether teachers are comfortable with this pedagogy. To determine teachers' current self-efficacy of STEM pedagogy, teachers in a southern state in the United States were asked to enlighten researchers into this phenomenon. Participants were K12 teachers (n = 119) from a public school district undertaking a district-wide STEM initiative. A measure of STEM teacher self-efficacy and a demographic questionnaire were administered online to participants. STEM teacher self-efficacy data were analyzed, along with demographic data, using descriptive discriminant analysis (DDA) and canonical correlation analysis (CCA). Results suggest some demographic variables are more predictive of STEM self-efficacy (gender, grade level taught, feelings of administrative support, and professional development sessions attended) than others (whether or not gifted courses are taught, age, and length of teaching experience. This data should be used by school administrators that seek to begin or improve STEM pedagogy in their schools.
BLE Controller Module for Wireless Sensor Networks
Authors:   , Vaswani Mohit Suresh
Published: February 2025

Sensors have been an integral part of our life since a long time. Traditionally, the transmit information to a data collection center through a physical wire. However, with the introduction of Bluetooth Low Energy (BLE) communication protocol, more research is being done into the field of wireless sensor networks (WSN). BLE was introduced to target low power applications. The CC2650 Launchpad designed by Texas Instruments (TI) can lead to a bulky final product. The aim was to design hardware for the CC2650 micro-controller with the aim of making it more compact for use in WSNs. A top-down approach was used wherein the available product is studied to identify the redundant and reverse engineer it to design a new product. A 2 layer printed circuit board (PCB) was designed which resulted in a 64 percent decrease in size compared to the Launchpad. Also, experiments were performed to test the proof of concept.
John Playford's The Division Violin: Improvisation and Variation Practice in English Violin Music of the Seventeenth Century
Authors:   , Chan Tzu-Ying
Published: February 2025

English publisher John Playford (1623-1686/1687) first published his "The Division Violin: Containing a Collection of Divisions Upon Several Grounds for the Treble-Violin" in 1684. The first edition of this violin collection contains 26 written-out examples of improvisation, serving as a living snapshot of the performance practice of the time. This research is based on the second edition, which Playford had expanded into 30 pieces for the violin, published in 1685. The purpose of this study is to investigate the art of improvisation in England during the late 17th century, focusing on Playford's "The Division Violin." The dissertation first surveys the development of English violin music in the 17th century. Then, the dissertation traces eight selected 16th-century Italian diminution manuals. This will help readers understand the progression of the Italian diminution and improvisation practice in the 16th century and how it relates to the English division of the 17th century. Finally, based on a thorough research of the 17th-century improvisatory style and rhetorical approach, the author of this study provides performance suggestions on "Mr. Farinell's Ground," No. 5 from "The Division Violin."
A Comprehensive Performance Guide for the Use of Advanced Technology in Euphonium Repertoire with Electronic Media through Analyses of Works by D. Edward Davis, Neal Corwell, and Lucy Pankhurst
Authors:   , Ray Irving
Published: February 2025

Solos for euphonium with electronic media present the unique challenge of incorporating an active, physical involvement in the live accompaniment through sound-altering technology such as guitar pedals or digital processors. Instructions for this solo genre are often vague and demand a general knowledge of how to use non-traditional devices. Due to the lack of information available on newly-composed pieces for this medium, students and professionals easily overlook the artistic merit of electroacoustic music. This dissertation provides a comprehensive performance guide that aids in the set-up and operation of advanced technology and presents a methodical approach to performing common musical and technical challenges found in modern euphonium repertoire with electronic media. Included in this dissertation are tables of common audio vocabulary and images of connectors, safety precautions, equipment recommendations with performance settings, a list of required connectors, adapters, cables, speakers, and amplifiers, performance set up diagrams, background information, and analyses of both the technical and musical aspects of each piece. In the appendices are signal flow charts, visual illustrations of polar recording patterns, and an updated catalog of published and unpublished original, adapted, and arranged euphonium solos with live electronics and electronic media accompaniment between 1970 and 2017.
Neurocognitive Effects of Gist Reasoning Training in Student-Athletes with Concussions, ADHD, and Learning Disabilities
Authors:   , Nguyen Thomas
Published: February 2025

Concussions, attention-deficit disorder (ADHD), and learning disabilities can adversely impact learning and academic achievement, particularly with respect to attention, memory, and executive functioning; fortunately, cognitive training can be beneficial and remediating these weaknesses. One such program, strategic memory advanced reasoning training (SMART), utilizes a top-down approach to train individuals in executive, higher-ordered thinking strategies including strategic attention, integration, and innovation to facilitate information synthesis and enhance cognitive efficiency. Thus, the purpose of the study is to examine whether SMART improved performances on various neuropsychological measures tapping into attention, processing speed, memory, and executive functioning for college student-athletes with neurological conditions (e.g., concussions, ADHD, LD). Student-athletes were randomly assigned to the SMART program or a "wait-list" control group and were administered a neuropsychological battery at baseline, immediately following the intervention, and after a four-month delay. Results showed that participants benefited from SMART with respect to working memory immediately following the intervention after controlling for baseline scores. The benefits of working memory also persisted after four months. Additionally, SMART was beneficial for improving attention, but only after four months after the intervention. The findings of the current study were consistent with previous studies which showed positive effects of SMART on working memory with a variety of populations (e.g., children, adolescents, older adults, Veterans, brain-injured patients); however, the current study did not see improved performance on other aspects of executive functioning which contradict prior research. Statistical differences between the present study and prior research regarding SMART may be explained in methodology, participant characteristics, research setting, and/or limitations. Future studies may include combining cognitive training as the intervention and utilizing neuroimaging alongside cognitive training to examine the relationship between structural/functional change with neuropsychological performance.
An Investigation of the Impact of Social Vulnerability Research on the Practice of Emergency Management
Authors:   , Williams Brian Don
Published: February 2025

This dissertation examines the extent to which social vulnerability, as studied by researchers across multiple disciplines, has influenced the practice of emergency management at the local level. This study addresses two major research questions to accomplish this goal. First, how do local emergency managers perceive and define social vulnerability? Second, what strategies do local emergency managers employ to reach and meet the needs of socially vulnerable populations? Semi-structured interviews were conducted in person or by phone with a sample of local emergency managers, city managers, and American Red Cross personnel from the Houston - Galveston and the South East Texas regions as defined by the respective Councils of Government. A modified grounded theory approach was used with a constant comparative method to identify themes for each research question. Triangulation was accomplished through secondary census data and supplemental interviews. The interview data reveal that social vulnerability research has had an indirect influence on the practice of emergency management at the local level. This influence is facilitated through state and federal policy, training, and plans development. Based on the interview data, four themes were identified that capture the various ways in which local emergency management officials perceive and define social vulnerability. These include vulnerability as poverty and culture, vulnerability as a lack of security, vulnerability as a moral imperative, and vulnerability as a lack of awareness and knowledge. In terms of strategies employed to address social vulnerability, the data suggest four themes: leaving it to the professionals, bringing in volunteers, leveraging protocols to build buy-in, and fostering flexibility. The findings reveal the importance in closing the knowledge gap between research and practice, because increased damage, harm, and death can occur when the social inequalities of everyday life are not addressed in the planning process by emergency managers. The findings also reveal that state and federal policy, training, and plans development are the most trusted sources by emergency managers to transfer knowledge to practice. Additionally, with the proliferation of emergency management degree programs at the undergraduate and graduate levels, higher education can potentially play a more active and visible role in bridging the gap between research and practice, particularly as it relates to social vulnerability.
Formation Control of Multi-Agent Systems
Authors:   , Mukherjee Srijita
Published: February 2025

Formation control is a classical problem and has been a prime topic of interest among the scientific community in the past few years. Although a vast amount of literature exists in this field, there are still many open questions that require an in-depth understanding and a new perspective. This thesis contributes towards exploring the wide dimensions of formation control and implementing a formation control scheme for a group of multi-agent systems. These systems are autonomous in nature and are represented by double integrated dynamics. It is assumed that the agents are connected in an undirected graph and use a leader-follower architecture to reach formation when the leading agent is given a velocity that is piecewise constant. A MATLAB code is written for the implementation of formation and the consensus-based control laws are verified. Understanding the effects on formation due to a fixed formation geometry is also observed and reported. Also, a link that describes the functional similarity between desired formation geometry and the Laplacian matrix has been observed. The use of Laplacian matrix in stability analysis of the formation is of special interest.
Neurological Responses to a Glucose Diet in Caenorhabditis elegans
Authors:   , Dumesnil Dennis
Published: February 2025

TRPV channels play a role in both mammalian insulin signaling, with TRPV1 expression in pancreatic beta-cells, and in C. elegans insulin-like signaling through expression of OSM-9, OCR-1, and OCR-2 in stress response pathways. In response to a glucose-supplemented diet, C. elegans are know to have sensitivity to anoxic stress, exhibit chemotaxis attraction, and display reduced egg-laying rate. Transcriptome analysis reveals that glucose stimulates nervous system activity with increased transcript levels of genes regulating neurotransmitters. Ciliated sensory neurons are needed for a reduced egg-laying phenotype on a glucose-supplemented diet. Egg-laying rate is not affected when worms graze on glucose-supplemented Delta-PTS OP50 E. coli, which is defective in glucose uptake. This suggests a possible sensory neuron obstruction by exopolysaccharides produced by standard OP50 E. coli on glucose, eliciting a starvation response from the worm and causing reduced egg-laying rate. Glucose chemotaxis is affected in specific TRPV subunit allele mutants: ocr-2(vs29) and osm-9(yz6), serotonin receptor mutants: ser-1(ok345) and mod-1(ok103), and G-alpha protein mutant: gpa-10(pk362). TRPV deletion mutants had no effect on glucose chemotaxis, alluding to the modality role pf TRPV alleles in specific sensory neurons. The role of serotonin in a reduced egg-laying rate with glucose remains unclear.