bluuebunny/crossref_metadata_2025 huggingface dataset:
Predicting surface deformation during mechanical attrition of metallic alloys
Authors: Cao Shan Cecilia , Zhang Xiaochun , Lu Jian , Wang Yongli , Shi San-Qiang , Ritchie Robert O.
Published: March 2019

Extensive efforts have been devoted in both the engineering and scientific domains to seek new designs and processing techniques capable of making stronger and tougher materials. One such method for enhancing such damage-tolerance in metallic alloys is a surface nano-crystallization technology that involves the use of hundreds of small hard balls which are vibrated using high-power ultrasound so that they impact onto the surface of a material at high speed (termed Surface Mechanical Attrition Treatment or SMAT). However, few studies have been devoted to the precise underlying mechanical mechanisms associated with this technology and the effect of processing parameters. As SMAT is dynamic plastic deformation process, here we use random impact deformation as a means to investigate the relationship between impact deformation and the parameters involved in the processing, specifically ball size, impact velocity, ball density and kinetic energy. Using analytical and numerical solutions, we examine the size of the indents and the depths of the associated plastic zones induced by random impacts, with results verified by experiment in austenitic stainless steels. In addition, global random impact and local impact frequency models are developed to analyze the statistical characteristics of random impact coverage, together with a description of the effect of random multiple impacts, which are more reflective of SMAT. We believe that these models will serve as a necessary foundation for further, and more energy-efficient, development of such surface nano-crystalline processing technologies for the strengthening of metallic materials.
Author Correction: Laquinimod treatment in the R6/2 mouse model
Authors: Ellrichmann Gisa , Blusch Alina , Fatoba Oluwaseun , Brunner Janine , Reick Christiane , Hayardeny Liat , Hayden Michael , Sehr Dominik , Winklhofer Konstanze F. , Saft Carsten , Gold Ralf
Published: March 2019

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
Copper transporter COPT5 participates in the crosstalk between vacuolar copper and iron pools mobilisation
Authors: Carrió-Seguí Àngela , Romero Paco , Curie Catherine , Mari Stéphane , Peñarrubia Lola
Published: March 2019

Copper (Cu) deficiency affects iron (Fe) homeostasis in several plant processes, including the increased Fe requirements due to cuproprotein substitutions for the corresponding Fe counterpart. Loss-of-function mutants from Arabidopsis thaliana high affinity copper transporter COPT5 and Fe transporters NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 3/4 (NRAMP3 and NRAMP4) were used to study the interaction between metals internal pools. A physiological characterisation showed that the copt5 mutant is sensitive to Fe deficiency, and that nramp3nramp4 mutant growth was severely affected under limiting Cu. By a transcriptomic analysis, we observed that NRAMP4 expression was highly induced in the copt5 mutant under Cu deficiency, while COPT5 was overexpressed in the nramp3nramp4 mutant. As a result, an enhanced mobilisation of the vacuolar Cu or Fe pools, when the other metal export through the tonoplast is impaired in the mutants, has been postulated. However, metals coming from internal pools are not used to accomplish the increased requirements that derive from metalloprotein substitution under metal deficiencies. Instead, the metal concentrations present in aerial parts of the copt5 and nramp3nramp4 mutants conversely show compensated levels of these two metals. Together, our data uncover an interconnection between Cu and Fe vacuolar pools, whose aim is to fulfil interorgan metal translocation.
Author Correction: An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process
Authors: Mesnage Robin , Agapito-Tenfen Sarah Z. , Vilperte Vinicius , Renney George , Ward Malcolm , Séralini Gilles-Eric , Nodari Rubens O. , Antoniou Michael N.
Published: March 2019

A correction has been published and is appended to both the HTML and PDF versions of this paper. The error has not been fixed in the paper.
Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization
Authors: Torán José Luis , López Juan Antonio , Gomes-Alves Patricia , Aguilar Susana , Torroja Carlos , Trevisan-Herraz Marco , Moscoso Isabel , Sebastião Maria João , Serra Margarida , Brito Catarina , Cruz Francisco Miguel , Sepúlveda Juan Carlos , Abad José Luis , Galán-Arriola Carlos , Ibanez Borja , Martínez Fernando , Fernández María Eugenia , Fernández-Aviles Francisco , Palacios Itziar , R-Borlado Luis , Vázquez Jesús , Alves Paula M. , Bernad Antonio
Published: March 2019

Adult cardiac progenitor/stem cells (CPC/CSC) are multipotent resident populations involved in cardiac homeostasis and heart repair. Assisted by complementary RNAseq analysis, we defined the fraction of the CPC proteome associable with specific functions by comparison with human bone marrow mesenchymal stem cells (MSC), the reference population for cell therapy, and human dermal fibroblasts (HDF), as a distant reference. Label-free proteomic analysis identified 526 proteins expressed differentially in CPC. iTRAQ analysis confirmed differential expression of a substantial proportion of those proteins in CPC relative to MSC, and systems biology analysis defined a clear overrepresentation of several categories related to enhanced angiogenic potential. The CPC plasma membrane compartment comprised 1,595 proteins, including a minimal signature of 167 proteins preferentially or exclusively expressed by CPC. CDH5 (VE-cadherin),  OX2G (OX-2 membrane glycoprotein; CD200), GPR4 (G protein-coupled receptor 4), CACNG7 (calcium voltage-gated channel auxiliary subunit gamma 7) and F11R (F11 receptor; junctional adhesion molecule A; JAM-A; CD321) were selected for validation. Their differential expression was confirmed both in expanded CPC batches and in early stages of isolation, particularly when compared against cardiac fibroblasts. Among them, GPR4 demonstrated the highest discrimination capacity between all cell lineages analyzed.
Colloidal analogues of polymer chains, ribbons and 2D crystals employing orientations and interactions of nano-rods dispersed in a nematic liquid crystal
Authors: M Muhammed Rasi , Pujala Ravi Kumar , Dhara Surajit
Published: March 2019

Robust control over the position, orientation and self-assembly of nonspherical colloids facilitate the creation of new materials with complex architecture that are important from technological and fundamental perspectives. We study orientation, elastic interaction and co-assembly of surface functionalized silica nano-rods in thin films of nematic liquid crystal. With homeotropic boundary condition, the nano-rods are predominantly oriented perpendicular to the nematic director which is different than the mostly parallel orientation of the micro-rods. The percentage of perpendicular nano-rods are significantly larger than the parallel nano-rods. The perpendicular nano-rods create very weak elastic deformation and exhibit unusual, out-of-plane, attractive interaction. On the other hand, the nano-rods oriented parallel to the director create strong elastic deformation and shows anisotropic, in-plane, dipolar interaction. In both orientations, the induced defects reside in the nano-rods. With the help of a dynamic laser tweezers and using nano-rods as building blocks we demonstrate colloidal analogues of linear polymer chains, ribbons and two-dimensional binary crystals.
Gadd45b mediates depressive-like role through DNA demethylation
Authors: Labonté Benoit , Jeong Yun Ha , Parise Eric , Issler Orna , Fatma Mena , Engmann Olivia , Cho Kyung-Ah , Neve Rachael , Nestler Eric J. , Koo Ja Wook
Published: March 2019

Animal studies using chronic social defeat stress (CSDS) in mice showed that brain-derived neurotrophic factor (BDNF) signaling in the mesolimbic dopamine (DA) circuit is important for the development of social aversion. However, the downstream molecular targets after BDNF release from ventral tegmental area (VTA) DA terminals are unknown. Here, we show that depressive-like behaviors induced by CSDS are mediated in part by Gadd45b downstream of BDNF signaling in the nucleus accumbens (NAc). We show that Gadd45b mRNA levels are increased in susceptible but not resilient mice. Intra-NAc infusion of BDNF or optical stimulation of VTA DA terminals in NAc enhanced Gadd45b expression levels in the NAc. Importantly, Gadd45b downregulation reversed social avoidance in susceptible mice. Together, these data suggest that Gadd45b in NAc contributes to susceptibility to social stress. In addition, we investigated the function of Gadd45b in demethylating CpG islands of representative gene targets, which have been associated with a depressive phenotype in humans and animal models. We found that Gadd45b downregulation changes DNA methylation levels in a phenotype-, gene-, and locus-specific fashion. Together, these results highlight the contribution of Gadd45b and changes in DNA methylation in mediating the effects of social stress in the mesolimbic DA circuit.
In vitro metabolic activation of vitamin D3 by using a multi-compartment microfluidic liver-kidney organ on chip platform
Authors: Theobald Jannick , Abu el Maaty Mohamed A. , Kusterer Nico , Wetterauer Bernhard , Wink Michael , Cheng Xinlai , Wölfl Stefan
Published: March 2019

Organ-on-chip platforms provide models that allow the representation of human physiological processes in cell-based miniaturized systems. Potential pre-clinical applications include drug testing and toxicity studies. Here we describe the use of a multi-compartment micro-fluidic chip to recapitulate hepatic vitamin D metabolism (vitamin D to 25-hydroxyvitamin D) and renal bio-activation (25-hydroxyvitamin D to 1,25-dihydroxyvitamin D) in humans. In contrast to cultivation in conventional tissue culture settings, on-chip cultivation of HepG2 and RPTEC cells in interconnected chambers, used to mimic the liver and kidneys, respectively, resulted in the enhanced expression of vitamin D metabolizing enzymes (CYP2R1, CYP27B1 and CYP24A1). Pump-driven flow of vitamin D3-containing medium through the microfluidic chip produced eluate containing vitamin D3 metabolites. LC-MSMS showed a strong accumulation of 25-hydroxyvitamin D. The chip eluate induced the expression of differentiation markers in HL-60 (acute myeloid leukemia) cells, assessed by qPCR and FACS analysis, in a manner similar to treatment with reference standards indicating the presence of fully activated 1,25 dihydroxyvitamin D, although the latter was not detected in the eluate by LC-MSMS. Interestingly, 25-hydroxyvitamin D by itself led to weak activation of HL-60 cells suggesting that 25-hydroxyvitamin D is also an active metabolite. Our experiments demonstrate that complex metabolic interactions can be reconstructed outside the human body using dedicated organ-on-chip platforms. We therefore propose that such systems may be used to mimic the in vivo metabolism of various micronutrients and xenobiotics.
Iron induces insulin resistance in cardiomyocytes via regulation of oxidative stress
Authors: Sung Hye Kyoung , Song Erfei , Jahng James Won Suk , Pantopoulos Kostas , Sweeney Gary
Published: March 2019

Iron overload is associated with various pathological changes which contribute to heart failure. Here, we examined mechanisms via which iron alters cardiomyocyte insulin sensitivity. Treatment of primary adult and neonatal cardiomyocytes as well as H9c2 cells with iron decreased insulin sensitivity determined via Western blotting or immunofluorescent detection of Akt and p70S6K phosphorylation and glucose uptake. Using CellROX deep red or DCF-DA probes we also observed that iron increased generation of reactive oxygen species (ROS), and that pretreatment with the superoxide dismutase mimetic MnTBAP reduced ROS production and attenuated iron-induced insulin resistance. SKQ1 and allopurinol but not apocynin reduced iron-induced ROS suggesting mitochondria and xanthine oxidase contribute to cellular ROS in response to iron. Western blotting for LC3-I, LC3-II and P62 levels as well as immunofluorescent co-detection of autophagosomes with Cyto-ID and lysosomal cathepsin activity indicated that iron attenuated autophagic flux without altering total expression of Atg7 or beclin-1 and phosphorylation of mTORC1 and ULK1. This conclusion was reinforced via protein accumulation detected using Click-iT HPG labelling after iron treatment. The adiponectin receptor agonist AdipoRon increased autophagic flux and improved insulin sensitivity both alone and in the presence of iron. We created an autophagy-deficient cell model by overexpressing a dominant-negative Atg5 mutant in H9c2 cells and this confirmed that reduced autophagy flux correlated with less insulin sensitivity. In conclusion, our study showed that iron promoted a cascade of ROS production, reduced autophagy and insulin resistance in cardiomyocytes.
Probing hair cell’s mechano-transduction using two-tone suppression measurements
Authors: Zhou Wenxiao , Nam Jong-Hoon
Published: March 2019

When two sound tones are delivered to the cochlea simultaneously, they interact with each other in a suppressive way, a phenomenon referred to as two-tone suppression (2TS). This nonlinear response is ascribed to the saturation of the outer hair cell’s mechano-transduction. Thus, 2TS can be used as a non-invasive probe to investigate the fundamental properties of cochlear mechano-transduction. We developed a nonlinear cochlear model in the time domain to interpret 2TS data. The multi-scale model incorporates cochlear fluid dynamics, organ of Corti (OoC) mechanics and outer hair cell electrophysiology. The model simulations of 2TS show that the threshold amplitudes and rates of low-side suppression are dependent on mechano-transduction properties. By comparing model responses to existing 2TS measurement data, we estimate intrinsic characteristics of mechano-transduction such as sensitivity and adaptation. For mechano-transduction sensitivity at the basal location (characteristic frequency of 17 kHz) at 0.06 nm−1, the simulation results agree with 2TS measurements of basilar membrane responses. This estimate is an order of magnitude higher than the values observed in experiments on isolated outer hair cells. The model also demonstrates how the outer hair cell’s adaptation alters the temporal pattern of 2TS by modulating mechano-electrical gain and phase.
Methylmercury induces the expression of chemokine CCL4 via SRF activation in C17.2 mouse neural stem cells
Authors: Kim Min-Seok , Takahashi Tsutomu , Lee Jin-Yong , Toyama Takashi , Hoshi Takayuki , Kuge Shusuke , Fujiwara Yasuyuki , Naganuma Akira , Hwang Gi-Wook
Published: March 2019

Methylmercury is an environmental pollutant that causes specific and serious damage to the central nervous system. We have previously shown that C-C motif chemokine ligand 4 (CCL4) protects cultured neural cells from methylmercury toxicity and expression of CCL4 is specifically induced in mouse brain by methylmercury. In this study, we examined the transcriptional regulatory mechanism that induces CCL4 expression by methylmercury using C17.2 mouse neural stem cells. The promoter region of the CCL4 gene was analyzed by a reporter assay, revealing that the region up to 50 bp upstream from the transcription start site was necessary for inducing expression of CCL4 by methylmercury. Nine transcription factors that might bind to this upstream region and be involved in the induction of CCL4 expression by methylmercury were selected, and the induction of CCL4 expression by methylmercury was suppressed by the knockdown of serum response factor (SRF). In addition, the nuclear level of SRF was elevated by methylmercury, and an increase in the amount bound to the CCL4 gene promoter was also observed. Furthermore, we examined the upstream signaling pathway involved in the induction of CCL4 expression by SRF, and confirmed that activation of p38 and ERK, which are part of the MAPK pathway, are involved. These results suggest that methylmercury induces the expression of CCL4 by activating SRF via the p38 and ERK signaling pathway. Our findings are important for elucidating the mechanism involved in the brain-specific induction of CCL4 expression by methylmercury.
PD-L1 expression combined with microsatellite instability/CD8+ tumor infiltrating lymphocytes as a useful prognostic biomarker in gastric cancer
Authors: Morihiro Toshiaki , Kuroda Shinji , Kanaya Nobuhiko , Kakiuchi Yoshihiko , Kubota Tetsushi , Aoyama Katsuyuki , Tanaka Takehiro , Kikuchi Satoru , Nagasaka Takeshi , Nishizaki Masahiko , Kagawa Shunsuke , Tazawa Hiroshi , Fujiwara Toshiyoshi
Published: March 2019

While the importance of programmed death-ligand 1 (PD-L1), mutation burden caused by microsatellite instability (MSI), and CD8+ tumor infiltrating lymphocytes (TILs) has become evident, the significance of PD-L1 expression on prognosis still remains controversial. We evaluated the usefulness of combined markers of PD-L1 and MSI or CD8+ TILs as a prognostic biomarker in gastric cancer. A total of 283 patients with gastric cancer were reviewed retrospectively. PD-L1 expression on >5% tumor cells was defined as PD-L1-positive. PD-L1-positive rate was 15.5% (44/283). PD-L1 positivity was significantly correlated with invasive and advanced cancer and also significantly correlated with MSI, whereas no significance was observed with CD8+ TILs. Kaplan–Meier analysis showed that PD-L1 positivity significantly correlated with a poor prognosis (p = 0.0025). Multivariate analysis revealed that PD-L1 positivity was an independent poor prognostic factor (hazard ratio [HR]: 1.97, p = 0.0106) along with diffuse histological type and lymph node metastases. Combinations of PD-L1 and MSI (HR: 2.18) or CD8+ TILs (HR: 2.57) were stronger predictive factors for prognosis than PD-L1 alone. In conclusion, combined markers of PD-L1 and MSI or CD8+ TILs may be more useful prognostic biomarkers in gastric cancer, and better clarify the immune status of gastric cancer patients.
Engineering an immunomodulatory drug-eluting stent to treat laryngotracheal stenosis
Authors: Duvvuri Madhavi , Motz Kevin , Murphy Michael , Feeley Michael , Ding Dacheng , Lee Andrew , Elisseeff Jennifer H. , Hillel Alexander T.
Published: March 2019

A rapamycin-eluting PLLA-PCL stent is deployed into a diseased mouse trachea to treat laryngotracheal stenosis.
Design and evaluation of conjugated polymers with polar side chains as electrode materials for electrochemical energy storage in aqueous electrolytes
Authors: Moia Davide , Giovannitti Alexander , Szumska Anna A. , Maria Iuliana P. , Rezasoltani Elham , Sachs Michael , Schnurr Martin , Barnes Piers R. F. , McCulloch Iain , Nelson Jenny
Published: March 2019

Solution processable p-type and n-type conjugated polymers with polar side chains enable fast charging in aqueous electrolytes and 1.4 V cell voltage.
Scalable fabrication of high-quality crystalline and stable FAPbI3thin films by combining doctor-blade coating and the cation exchange reaction
Authors: Adjokatse Sampson , Fang Hong-Hua , Duim Herman , Loi Maria Antonietta
Published: March 2019

This study highlights the importance of a scalable blade coating technique and the ion exchange reaction in fabricating high-quality perovskite thin films.
Directly ambient pressure dried robust bridged silsesquioxane and methylsiloxane aerogels: effects of precursors and solvents
Authors: Chen Dangjia , Gao Hongyi , Liu Panpan , Huang Pei , Huang Xiubing
Published: March 2019

Aerogels prepared with different precursors and solvents using a facile procedure.
Fully automated peptide radiolabeling from [18F]fluoride
Authors: Davis Ryan A. , Drake Chris , Ippisch Robin C. , Moore Melissa , Sutcliffe Julie L.
Published: March 2019

A fully automated approach for peptide radiolabeling from [18F]fluoride, provides an efficient process that is amenable to GMP production of [18F]F-peptide radiotracers.
Divergent total syntheses of five illudalane sesquiterpenes and assignment of the absolute configuration
Authors: Zeng Zhixiong , Zhao Yifan , Zhang Yandong
Published: March 2019

Transition metal catalyzed [2+2+2] cycloaddition and aromatic C–H oxygenation enables concise, divergent total syntheses of five bioactive illudalane sesquiterpenes.
Exogenous-oxidant-free electrochemical oxidative C–H phosphonylation with hydrogen evolution
Authors: Yuan Yong , Qiao Jin , Cao Yangmin , Tang Jingmei , Wang Mengqin , Ke Guojuan , Lu Yichen , Liu Xue , Lei Aiwen
Published: March 2019

We herein report a versatile and environmentally friendly electrochemical oxidative C–H phosphonylation protocol.
Copper-catalyzed one-pot three-component thioamination of 1,4-naphthoquinone
Authors: Zeng Fan-Lin , Chen Xiao-Lan , He Shuai-Qi , Sun Kai , Liu Yan , Fu Rui , Qu Ling-Bo , Zhao Yu-Fen , Yu Bing
Published: March 2019

A concise one-pot three-component thioamination of 1,4-naphthoquinone with thiols and amines was developed to synthesize 2-amino-3-thio-1,4-naphthoquinones.
CuxNiy alloy nanoparticles embedded in a nitrogen–carbon network for efficient conversion of carbon dioxide
Authors: Tan Dongxing , Zhang Jianling , Cheng Xiuyan , Tan Xiuniang , Shi Jinbiao , Zhang Bingxing , Han Buxing , Zheng Lirong , Zhang Jing
Published: March 2019

CuxNiy alloy nanoparticles embedded in a nitrogen–carbon network can effectively convert CO2 into CO under mild conditions.
Crystal structure of dimeric Synechococcus spermidine synthase with bound polyamine substrate and product
Authors: Guédez Gabriela , Pothipongsa Apiradee , Sirén Saija , Liljeblad Arto , Jantaro Saowarath , Incharoensakdi Aran , Salminen Tiina A.
Published: March 2019

Spermidine is a ubiquitous polyamine synthesized by spermidine synthase (SPDS) from the substrates, putrescine and decarboxylated S-adenosylmethionine (dcAdoMet). SPDS is generally active as homodimer, but higher oligomerization states have been reported in SPDS from thermophiles, which are less specific to putrescine as the aminoacceptor substrate. Several crystal structures of SPDS have been solved with and without bound substrates and/or products as well as inhibitors. Here, we determined the crystal structure of SPDS from the cyanobacterium Synechococcus (SySPDS) that is a homodimer, which we also observed in solution. Unlike crystal structures reported for bacterial and eukaryotic SPDS with bound ligands, SySPDS structure has not only bound putrescine substrate taken from the expression host, but also spermidine product most probably as a result of an enzymatic reaction. Hence, to the best of our knowledge, this is the first structure reported with both amino ligands in the same structure. Interestingly, the gate-keeping loop is disordered in the putrescine-bound monomer while it is stabilized in the spermidine-bound monomer of the SySPDS dimer. This confirms the gate-keeping loop as the key structural element that prepares the active site upon binding of dcAdoMet for the catalytic reaction of the amine donor and putrescine.
Rat mRNA expression profiles associated with inhibition of ischemic acute kidney injury by losartan
Authors: Wu Yijin , Peng Wenying , Wei Ru , Zhou Yanhe , Fang Miaoxian , Liu Siyi , Deng Yujun , Yin Qi , Ouyang Xin , Hu Linhui , Hou Yating , Chen Chunbo
Published: March 2019

Objective: Losartan was reported to inhibit the progression of acute kidney injury (AKI), but little is known about the underlying pharmacological mechanisms. In the present study, the mRNA expression profiles in ischemic AKI rat kidney altered by losartan treatment were analyzed by next-generation deep sequencing technology.

Methods: Ischemia and reperfusion treatment was applied to induce AKI in Sprague–Dawley (SD) rats. The urea and creatinine contents in rat blood were measured. H&E staining was performed to evaluate the histological alteration of rat kidney tissues under a microscope. The TUNEL method was applied to analyze apoptosis in rat kidney tissues. The mRNA profiles in rat kidney were analyzed using next-generation deep sequencing. Differential gene expression was confirmed by quantitative qRT-PCR.

Results: The rat model of AKI induced by ischemia and reperfusion showed significant increases in urea and creatinine levels, accompanied by a disrupted kidney tubular structure and renal cell apoptosis. Losartan treatment effectively inhibited the changes in urea and creatinine, tubular structure, and apoptosis in AKI rat kidney. A large number of mRNAs were found to be differentially expressed in the kidneys of AKI rats treated with losartan, which are involved in multiple processes and signaling pathways. The expression of nine differentially expressed genes such as monocyte chemoattractant protein-1 (CCL2) and suppressor of cytokine signaling 3 (SOCS3) was confirmed by qRT-PCR and Western blot.

Conclusion: Losartan caused significant alterations in the gene expression profile in AKI rat kidney, which mediated its anti-AKI effects.
miR-500 promotes cell proliferation by directly targetting LRP1B in prostate cancer
Authors: Zhang Zhaoli , Cui Ran , Li Hui , Li Jinlong
Published: March 2019

Accumulating evidence suggests that miRNAs play a crucial role in the development of prostate cancer (PC); however, the role of miR-500 in PC remains poorly understood. The data presented here reveal abnormal increases in miR-500 expression in PC tissues and cell lines. Suppression of miR-500 expression significantly inhibited the proliferation of PC-3 and LnCap cells and was negatively regulative with low-density lipoprotein receptor-related protein 1B (LRP1B). Increased cell cycle arrest at the G1 stage and decreased protein expression of cyclinD1 and CDK2 was observed in response to miR-500 knockdown in PC-3 and LnCap cells, in combination with LRP1B overexpression. LRP1B was identified as a target of miR-500 and was significantly decreased in PC tissues. Taken together, these findings demonstrate that miR-500 plays an important role in the proliferation of PC cells via the inhibition of LRP1B expression.
Biotemplated facile synthesis of three‐dimensional micro/nanoporous tin oxide: improving the flammable and mechanical properties of flexible PVC
Authors: Meng Weihua , Wu Weihong , Zhang Weiwei , Cheng Luyao , Jiao Yunhong , Qu Hongqiang , Xu Jianzhong
Published: March 2019

In this study, a flame retardant [Pollen‐type SnO2 (P‐SnO2)] with a micro/nanoporous structure was synthesised by the simple immersion–calcination method using pollen as a biological template. When applied into flexible polyvinyl chloride (PVC), PVC composite adding P‐SnO2 (PVC/P‐SnO2) exhibited much better mechanical property than that adding commercial SnO2 (PVC/[C‐type SnO2 (C‐SnO2)] for the results that the tensile strength and impact toughness are increased by 11.3 and 37.9%, respectively. Moreover, PVC/P‐SnO2 has always the same flame retardancy and smoke suppression abilities compared with PVC/C‐SnO2. It provides a method using biotemplate for preparing metal oxide flame retardants with both great flame retardant and toughening properties.
Applications of bolted steel plates to shear strengthening of RC beams
Authors: Sudarsana I Ketut , Chandra Sajana I Putu , Ngurah Oka Suputra I Gusti
Published: March 2019

Many reinforced concrete beams have been found deficiencies in shear due to lack of shear reinforcement. Few researches have been done to investigate shear strengthening of low concrete strength of RC beams using bolted steel plates. This paper presents experimental results on the behaviour of RC beams strengthening using full steel plates along the shear span fixed with bolts. Nine RC beams having dimension of 150x200x1500mm were tested to failure on simply supports with four point loadings setup. The beams were reinforced with tension rebars area of 289.8 mm2 (2D10+1D13) and transvers reinforcement of Ø6-125. Two dial gauges were installed at the beam middle span to measure beam deflection during the test. The results show that the applications of steel plate and bolts as external reinforcement increase beam shear capacity, stiffness and delay the occurrence of first diagonal cracks. Beams stregnthened with U-shape plates gave better performances than the beams with two pieces of L-shape plates due to better achorages.
The effects of steel fiber waste tyre on properties of high strength fly ash concrete
Authors: Fauzan  , Kurniawan Rudy , Lovina A. N Claudia , Fitrah N Oscar , Basenda T Putri
Published: March 2019

The utilization of steel fiber from waste tyres can be an alternative to reduce waste tyres due to the increase of tyre production in Indonesia annually. Steel fiber from waste tyre can be added to concrete mix to improve the concrete properties. In this study, the effects of steel fiber waste tyre (SFWT) on high strength concrete containing fly ash was investigated experimentally. The content of fly ash in the high strength concrete is 30% of being partially replaced the cement weight. Steel fiber waste tyres are obtained from extracting the steel wire of the waste tyres and then cut into 4 cm long. The addition of SFWT on the high strength fly ash concrete is 0.5%, 1.0%, 1.5%, and 2% by concrete volume. The mechanical properties of concrete such as compressive strength, tensile strength, and flexural strength are tested at day 28. The test results show that the addition of 2 % SFWT on high strength fly ash concrete increase the compressive strength, tensile strength, and flexural strength of the concrete by around 9.99 %, 63.75 %, 18.18 %, respectively.
3D-Finite element modeling of lead rubber bearing using high damping material
Authors: Basshofi Habieb Ahmad , Tavio Tavio , Milani Gabriele , Wijaya Usman
Published: March 2019

Lead Rubber Bearing (LRB) has been widely applied for seismic protection of mid and high-rise buildings around the world. Its excellent energy dissipation becomes the most important aspect of this isolation system thanks to the plasticity and recovery behavior of the lead core. Aiming to develop a deeper knowledge on the behavior of LRB’s, a 3D detailed finite element (FE) modeling is performed in Abaqus FE software. Some important parameters involved in the model are plasticity of the lead core and hyper-elasticity and viscosity of the rubber material. The parameters for rubber material are derived from the results of experimental works in the laboratory, including uniaxial tensile test and relaxation test. The bearing model is then subjected to a cyclic shear-test under constant vertical load. The result of the 3D-FE model is then compared with the analytic-Abaqus model for LRB isolators, developed in the literature. Finally, both 3D-FE model and analytic model result in a good agreement on the shear behaviour of the presented LRB.
Structural evaluation of Ikhwatun shelter building constructed on liquefaction potential area in Padang city, Indonesia
Authors: Fauzan  , Hakam Abdul , Yuliet Rina , Vincensius Osman Jonathan
Published: March 2019

Indonesia has often experienced seismic natural disasters such as earthquakes and tsunamis especially Padang City, the capital city of West Sumatera, Indonesia. In order to face the future earthquake and tsunami disaster, the local government has built a number of vertical evacuation shelters. One of the shelters is Ikhwatun shelter building located in Koto Tangah Subdistrict of the Padang City. The shelter was built near to the coastal and expected has liquefaction potential. This study is conducted in order to evaluate the shelter to restrain the earthquake and tsunami loads. The building is made of the reinforced concrete structure with the floor area of 2680 m2 and the high of 22.78 m. Based on the result of the soil evaluation, it was found that the soil deposit in the shelter has high liquefaction potential. Therefore, the upper and lower structures are analyzed using special response spectrum of the earthquake loads for soil liquefaction, which is 1.5 higher than those on the non-soil liquefaction. The analysis result shows that the beams, columns, and foundations are all not able to resist the applied tsunami loads. It is suggested that the building to be strengthened before being used as a vertical evacuation shelter.
Seismic performance of building reinforced with CFRP bars
Authors: Suriyati  , Ridwan  , Djauhari Zulfikar , Romey Sitompul Iskandar
Published: March 2019

Over the past two decades, laboratory tests have suggested that fibre reinforced polymer (FRP) bars can replace steel bars as internal reinforcement in concrete structures, especially those are subjected to aggressive environments. FRP bars has potential for replacing steel bars as these material are corrosion-resistant and have high tensile strength, make it suitable for use as structural reinforcement. Unfortunately, the application of FRP for reinforcing structures in Indonesia is dubious. This paper aims to study the behaviour of reinforced concrete (RC) building reinforced with Carbon FRP (CFRP) and steel bars. The observed building was designed for Pekanbaru, according to SNI 2847:2013 for design of structural RC with steel bars reinforcement and the ACI 440.1R-15 for design and construction of structural RC with FRP bars. Two reinforcedconcrete moment resisting frames, extracted from 6 storey heights building with rectangular floor plan, were designed for the city of Pekanbaru. The building had the typical storey height of 3.6 m except for the first floor where the column length was 4.0 m. Two frames were investigated, including the frame without (Frame A) and with shear wall (Frame B). Pushover analysis was conducted to establish the design force and the design deformation demands. The results show that the amount of bar areas needed for reinforcing the building with CFRP bars was significantly reduced. Moreover, the maximum forced demand attained for the building reinforced with CFRP bars were enhanced by about 1.9 times compared to that of reinforced with steel bars.
Utilization of electronic scraps on making a concrete brick
Authors: Sahan Yusnimar , Lusi Jayanti Dewi , Dwi Anggriana Nurul
Published: March 2019

Electronic scraps is becoming one of the environment issues globally, including in Indonesia. Innovation in the electronic technologies is growing very rapidly (printer, computers, mobile phones, etc.) and consumers tend to replace électronic goods in a relatively short time. However, the development of electronic tools is not comparable with the invention of its recycling technology, so landfill becomes the first choice to over come the number of electronic scraps that are increasing every year. Most of the electronic scraps are made from ± 30-65% engineering thermoplastics and the rest are metal alloys. In this research, an alternative effort has been made for utilization of thermoplastics as a partial filler replacement for fine sand into commercially valuable products, a concrete brick. As a result, the maximum compressive strength of the concrete brick is 219.968 kg/cm2 by using thermoplastics ± 10% higher than a concrete brick normally, it does not cause scratches when scratched with nails, also not cause a loud sound when two bricks beaten one with the other and a punch is not too hard.
Progressive collapse of regular and irregular reinforced concrete moment frame
Authors: Yolanda Ardian , Djauhari Zulfikar , Ridwan  , Yuniarto Enno
Published: March 2019

A technique to evaluate the potential progressive collapse of reinforced concrete structure was conducted in this study. The analysis involved the removal of several columns on critical location of the building according to General Services Administration (GSA) 2013 provision. In each analysis, the demand-capacity ratios (DCRs) of structural elements were examined and compared to the defined acceptance criteria. To avoid structural building collapse progressively, DCR ratio of regular and irregular buildings should be less than 2 and 1.5, respectively. The result showed that the structure did not collapse with the removal single column only. Further to this finding, several columns need to be removed so that it collapsed progressively. In the case of regular structure, progressive collapse occurred after removing five columns on the side of the regular structure, with the maximum DCR of 4.66. In the case of irregular structure, progressive collapse occurred after removing four columns on the horizontal side in the middle of structure with the maximum DCR of 3.44.
Determination of prioritization for maintenance of the upper structure of truss bridge
Authors: Fitriani Heni , Surya Pratama M. Ade , Idris Yakni , Tanzil Gunawan
Published: March 2019

Bridge maintenance is one of the major issues of infrastructure problems. Deterioration of a bridge’s structure will continuously increase without proper maintenance. This condition will adversely affect the service life of a bridge. Moreover, the damage will also have a direct impact on structural and functional failure of the bridge. This paper aims at identifying the damages of truss bridges and determining the most significant criteria and sub-criteria used in prioritizing bridge maintenance. Analytical Hierarchy Process (AHP) was used to assess the most important criteria that give significant weight to bridge maintenance analysis. The objects of research were nine truss bridges with a wide range of types and levels of damage. It was found that there were approximately 900 m' of components damaged at the railing of Baruga Bridge and 227 m' truss damages due to poor quality of the galvanized paint. Furthermore, based on the analysis, the most significant criteria were the level of damage (27.6%), the technical aspects (25.7%), the finance (21%), the vehicle load (13.6%) and the resources (12%). The results of this research showed important findings in determining the priority scales for bridge repair and maintenance systems.
Study of cold formed steel beam column joint to resist lateral load
Authors: Desmaliana Erma , Herbudiman Bernardinus , Mentari Ulfayani Andi , Ahmad Shobur Gunawan Fauzi
Published: March 2019

Cold Formed Steel (CFS) is one of the materials that recently used in building structures. There are many advantages of CFS compared with other materials, such as lightweight, high tensile strength, and fast construction. Hollow Structural Sections (HSS) is the most commonly used as beam and column CFS sections. The purposes of this study are determining the behavior and capacity of CFS beam column joint under lateral load with manual calculation, numerical analysis and experimental. The test specimens used 1 mm thick, 4x4cm HSS for column and 2x4cm HSS for beam. Beam and column connected by two single angle plates placed above and below the beam sides. Both parallel and staggered fastener configurations used in this experiment. Four 4 mm diameter bolts used in this connection. Monotonic static loading applied on beam for modelling the lateral load. The experimental results show the behavior and the capacity of the beam column joint with parallel fastener configurations. The numerical results show that the staggered fastener configuration has better performance compared with the parallel fastener configuration. The CFS beam column joint are adequate to resist the lateral load and feasible to be apply as structural components in building structures.
Dominant factors influencing project quality in the radioactive minerals processing pilot plant construction
Authors: Madyaningarum Nunik , Ali Berawi Mohammed , Gunawan  , Sukadana I Gede
Published: March 2019

Monitoring and supervision of factors that predominantly affect the successful implementation of quality management systems on the project will improve the quality of construction that provides a safety factor in the construction of a pilot plant nuclear fuel processing, to determine those factors is the aim of this research. The separation of Uranium, Thorium, and Rare Earth Metals Pilot Plant was used as a case of the research. Adopted descriptive research design were the primary data collected using questionnaires. Data collected analysed thought multiple linear regressions that only done on factors that have correlation strong – very strong. International Standard Organisation series 9001:2015 and International Atomic Energy Agency safety standards series No. GS-R3 were used as independent variables (X), contamination level was used as dependent variable (Y) that reflected project quality. The result is there are three factors that statistically significant influencing project quality: leadership and commitment, project planning, and safety culture. Those factors influencing the achievement of construction quality of the pilot plant uranium, thorium and rare earth element i.e. 92.3%, while the remaining 7.7% (100-92.3%) is determined by other variables.
Risk management system model to improve the reputation of oil and gas companies in the Java island - Indonesia
Authors: Nova Nevila Rodhi , Artama Wiguna I Putu , Nadjadji Anwar
Published: March 2019

Risk management has been widely studied and applied in oil and gas pipeline projects, but the reality is that the impacts still occur. This is due to the ineffectiveness of existing risk management applications, so of course in this case an effective risk management system is needed, in which risk management must pay attention to all aspects that exist, both internal aspects, external aspects, and other aspects that can affect and influenced by existing risks and risk management not only can be used as a preventive method. But it can also support sustainable development targets. This paper presents the application of risk management by oil and gas companies in Indonesia that has been adapted to ISO 31000:2009 as a framework that can integrate various other management processes, including the management of HSE (Health, Safety, and Environment) risk in the hope of that sustainable development can be achieved . But in the reality it can not be denied that the activities that continue to this day still cause negative impact, especially for the environment. International oil and gas companies nowadays place more emphasis on preventive measures than the methods of mitigation. Thus it shows that the concept of sustainable development has not been fully considered in risk management applications. The existence of a continuous negative impact would greatly affect the credibility of oil and gas companies. Based on the results of research conducted with the hybrid method and analyzed with the help of System Dynamics it can be concluded that there are 3 (three) factors that can affect reputation risk, these factors are social, environmental, and economic and the model built in this study shows that it will can help the oil and gas company players to predict and improve the company’s reputation.
Improving Occupational Health and Safety (OHS) implementation in construction project in Bali
Authors: Gusti Dharmayanti , Sien Pramana , Astawa Diputra , Ariany Frederika
Published: March 2019

Optimal Occupational Health and Safety (OHS) implementation to achieve zero accident becomes the demand for construction service providers in realizing a qualified infrastructure, including for construction industry in Bali and Indonesia as general. This study aims to investigate the barriers of OHS implementation faced by contractors in Bali, and to formulate the efforts to improve the implementation. Collected data from a questionnaire survey were analysed using Analytic Hierarchy Process (AHP) to determine the priority level of the identified barriers. The results suggested that the barriers on the implementation of OHS which was sorted from the most important included Limited funding for OHS (3.231), Low OHS priority by company management (2.020), Low culture and discipline to implement OHS (1.031), Lack of knowledge about OHS (0.725), Weak supervision (0.478), Weak application of sanctions from the company (0.340), and Contractor forced to work until late at night (0.230). The related efforts were formulated based on semi-structured interviews recommended that the cost of implementing OHS including the required methods and equipment should be covered in the planning stage, and socialization, supervision and strict sanctions against violations of OSH implementation needs to be applied.
Improving relationship quality between main contractors and sub-contractors in Indonesian Infrastructure Projects
Authors: Agustiawan Yosi , Coffey Vaughan , Lamari Fiona
Published: March 2019

This paper has two main sections, the first of which provides a summarized review of the literature focusing on existing studies of the adversarial relationships between main and sub-contractors in project execution phases. Such relationships become hurdles for the construction organizations to achieve quality relationships. Organizational culture influences these relationships due to its constituent elements of value, leadership style, procedures, and routine, as well as defining success or failure. Drawing from the first section, the second section presents the details of the research project being undertaken. Suitable research methods have been developed to meet the research objectives eliciting based on fundamental questions. Primary data collection will use a mixed methods approach, i.e., questionnaire surveys and semi-structured interviews to obtain respondents opinions related to social interactions in Indonesian infrastructure projects. The project-based organizational culture assessment of Zou (2014) and relationship quality evaluation of Jiang (2016) are utilized in this study. Statistical software will be used to analyze most data expected to be obtained in the future and then the results will be discussed to develop a framework to improve relationship quality in achieving project performance.
Developing the simulation model towards sustainability of implementing performance-based contract
Authors: Artama Wiguna I Putu , Anwar Nadjadji , Teki Tjendani Hanie
Published: March 2019

Performance-based contracts (PBC), one kind of the delivery system has been conducted in Indonesia starting in 2011. The aim of the implementation of PBC in Indonesia is to solve the premature deterioration of national roads in Indonesia that still occur to date and to achieve the best level of service. Implementation of performance-based contracts is not as easy as imagine since many contractors still don’t understand to control the risk if they are given work with performance-based contracts. In this study used the system dynamic approach, the choice of this method because PBC is a long-term contract, where past events can affect current and future events, as well as future events, can be avoided by evaluating today’s events and events in the past. In this paper will show the interface generated after the base model Stock Flow Diagram (SFD) is verified. After the model has been validated, the model is ready as a simulation tool to get the best performance-based contracting mechanism and implementation in Indonesia. So, it can be used in developing countries.