bluuebunny/crossref_metadata_2025 huggingface dataset:
A Hybrid Model for 30-Day Syncope Prognosis Prediction in the Emergency Department
Authors: Dipaola Franca , Gatti Mauro , Menè Roberto , Shiffer Dana , Giaj Levra Alessandro , Solbiati Monica , Villa Paolo , Costantino Giorgio , Furlan Raffaello
Published: December 2023

Syncope is a challenging problem in the emergency department (ED) as the available risk prediction tools have suboptimal predictive performances. Predictive models based on machine learning (ML) are promising tools whose application in the context of syncope remains underexplored. The aim of the present study was to develop and compare the performance of ML-based models in predicting the risk of clinically significant outcomes in patients presenting to the ED for syncope. We enrolled 266 consecutive patients (age 73, IQR 58–83; 52% males) admitted for syncope at three tertiary centers. We collected demographic and clinical information as well as the occurrence of clinically significant outcomes at a 30-day telephone follow-up. We implemented an XGBoost model based on the best-performing candidate predictors. Subsequently, we integrated the XGboost predictors with knowledge-based rules. The obtained hybrid model outperformed the XGboost model (AUC = 0.81 vs. 0.73, p < 0.001) with acceptable calibration. In conclusion, we developed an ML-based model characterized by a commendable capability to predict adverse events within 30 days post-syncope evaluation in the ED. This model relies solely on clinical data routinely collected during a patient’s initial syncope evaluation, thus obviating the need for laboratory tests or syncope experienced clinical judgment.
An Inquiry into the Characteristics of Carbon Emissions in Inter-Provincial Transportation in China: Aiming to Typological Strategies for Carbon Reduction in Regional Transportation
Authors: Yang Yuhao , Yan Fengying
Published: December 2023

The low-carbon development of the transportation sector is crucial for China to achieve its national goals of carbon peaking and carbon neutrality. Since China is a vast country with unbalanced regional development, there are considerable differences in the levels of carbon dioxide emissions from the transportation sector across regions. Therefore, revealing the influencing factors that shape the characteristics of transportation carbon dioxide emissions (TCO2) can inform tailored sub-national carbon reduction strategies based on local conditions, which is an important technical approach for achieving national goals. Based on an extended Kaya identity, we derived indicators of the impacts on provincial TCO2 from factors such as economic development, population density, energy structure, transportation efficiency, technology research and development (R&D), infrastructure construction, transportation operation conditions, and residents’ transportation behavior. Using a multi-indicator joint characterization method, we explored the characteristics of provincial TCO2 in China in 2019. By applying Ward’s method to hierarchical clustering, the thirty provinces of China were classified into six characteristic types (Types I to VI). Based on the total TCO2 (TC), the intensity of TCO2 (TI), and the per capita TCO2 (TP) calculated for each province in 2019, the priority control directions and indicators for carbon reduction in each type were obtained through relative relationships with provincial averages and correlation analysis with the indicators. Specifically, Type I and Type IV can be categorized as TP-controlled, Type II and Type III as TC-controlled, and Type V and Type VI as TI-controlled. Finally, we provided typological strategies and key performance indicators (KPIs) relevant to local governments to better achieve carbon reduction goals in each provincial type. It can promote cooperative development and collaborative governance in carbon reduction across regions and the unified implementation of China’s dual-carbon goals.
Stative vs. Eventive Participles in an Arbëresh Variety under the Influence of the Italian Language
Authors: Turano Giuseppina
Published: December 2023

In this paper, I explore the properties and the uses of the past participles in the Arbëresh variety of S. Nicola dell’Alto, an Albanian dialect still spoken in Southern Italy, which has been in contact with Italo-Romance varieties for more than five centuries. The data are discussed in comparison to standard Albanian and the contact language, Italian. In Albanian grammar, there is only one type of participle: the past participle. It has both verbal and adjectival properties. As a verbal form, the participle is used in compound and in periphrastic tenses, in combination with both the auxiliaries KAM ‘have’ and JAM ‘be’. It can also be used in combination with other particles to create non-finite verbal forms such as gerund or infinitive or to build up temporal expressions. Finally, it can also be used after some modal impersonal verbs. Verbal participles never show agreement. Albanian participles can also be adjectival. All the adjectives derived by a participial verb take a linking article and always agree with the noun they modify in gender, number, Case and definiteness. The formal distinction of the verbal participles from adjectival participles seems to correlate with the aspectual properties of the construction: a verbal participle appears in eventive structures, whereas an adjectival participle occurs in stative structures. But, as we shall see, this is not always the case. Arbëresh participles have maintained the same morphological and syntactical properties of Albanian. They can be used both in stative and in eventive contexts, but in Arbëresh eventive passives, which are built up as in Italian rather than as in Albanian, the adjectival participles are always inflected. Agreement is obligatory in all the contexts where it is in Italian. This is a clear contact-induced change. The data presented in this paper show that Arbëresh, on the one hand, preserves features of Albanian grammar, whereas, on the other hand, it has undergone changes under the influence of the surrounding Italo-Romance varieties.
Ethnic Rootedness and Social Affiliations at the Interface with Linguistic Performativity: Evidence from Americans of Southwest Asian or North African Descent
Authors: Sheydaei Iman
Published: December 2023

This study explores the relationship between linguistic behaviors of Americans of Southwest Asian or North African descent (SWANA Americans) and their ethnic rootedness, religion, and locality. SWANA Americans are an understudied community in the field of sociolinguistics but could be highly visible in society. SWANA Americans have historically and legally been classified as white in the US despite the social perception that they are not white. The linguistic analysis in the present paper will reflect the social discrepancies between the top-down perspective of assigning all SWANA Americans a statistical race category versus the bottom-up perspective of examining the social implications of this community’s nuanced internal composition differences. Labovian Sociolinguistic Interviews were conducted with 54 SWANA Americans in the Upper Midwest and Southern California, and an ethnic rootedness metric was designed to measure individual speakers’ ethnic rootedness. The results show that higher ethnic rootedness, being a Muslim (in the more careful speech style), and being from Dearborn, MI, are significant predictors of higher rates of “reracializing” indexically bleached ethnically affiliated lexicon (words such as Ali, Muslim, Iraq, Mohammad, etc.) in the speakers’ English speech.
Windfall Profit Taxation in Europe (and Beyond)
Authors: Greggi Marco , Miotto Anna
Published: December 2023

In 2022, the European Commission introduced, for the first time in its history, a windfall profit tax to be applied on “excessive” profits realized by qualified businesses operating in the “Oil and Gas” sector. Immediately after its implementation, questions arose as to its sustainability and its consistency with constitutional principles of the different member states regulating the domestic power to tax. To assess the consistency with the aforesaid rules, the article samples two countries, inside and outside the EU (Italy and Australia, respectively), and the historical precedents of the matter. Italy has been chosen due to the particularly stringent set of principles regulating the power of the legislature to tax, and Australia has been chosen because of the long-standing experience with superprofit taxes. In most of the scenarios analyzed, one common feature emerged: the complexity in defining the “Extra” nature of the profits and, consequently, the uncertainties in the calculation of the taxable base. In the case of Italy, for instance, the legislator had to intervene in several different moments to fine-tune the taxable base and restore certainty to the tax system. As a conclusion, while the taxation of extra profits should not per se be disregarded, its implementation demands a more robust and precise legal framework together with the understanding that the introduction of such a levy would be a one-way journey for the tax systems: windfall profits taxes would be here to stay.
Effect of Thermomechanical Loading at Low Temperatures on Damage Development in Glass Fiber Epoxy Laminates
Authors: Krzak Anna , Al-Maqdasi Zainab , Nowak Agnieszka J. , Joffe Roberts
Published: December 2023

Due to the high interest in the use of glass/epoxy laminates in aerospace applications, aviation, and as cryogenic tanks, it is crucial to understand the behavior of composites under challenging environmental conditions. Polymer composites are exposed to low temperatures, including cryogenic temperatures, which can lead to the initiation of microdamage. This paper investigates damage initiation/accumulation and its influence on the properties of cross-ply woven glass fiber epoxy composites at low temperatures compared to room temperature conditions. To evaluate the influence of a low-temperature environment on the mechanical performance of glass fiber reinforced epoxy composite (GFRP) laminates, three types of test campaigns were carried out: quasi-static tensile tests and stepwise increasing loading/unloading cyclic tensile tests at room temperature and in a low-temperature environment (−50 °C). We demonstrated that the initial stiffness of the laminates increased at low temperatures. On the other hand, there were no observed changes in the type or mechanism of developed damage in the two test conditions. However, the reduction in stiffness due to the accumulated damage was more significant for the laminates tested at low temperatures (~17% vs. ~11%). Exceptions were noted in a few formulations where the extent of damage at low temperatures was insignificant (<1%) compared to that at room temperature. Since some of the studied laminates exhibited a relatively minor decrease in stiffness (~2–3%), we can also conclude that the formulation of matrix material plays an important role in delaying the initiation and formation of damage.
Diagnostics of Large-Panel Buildings—An Attempt to Reduce the Number of Destructive Tests
Authors: Wardach Maciej , Krentowski Janusz Ryszard
Published: December 2023

Structural condition diagnostics provides the basis for decision making regarding the possibility of continued safe operation, necessary reinforcement, repair work, and in extreme cases, dismantling of the structure. The most reliable results concerning the condition and strength of materials are provided by destructive testing. However, these tests are very time-consuming, costly, and difficult to perform on in-service facilities. In addition, they involve the need to obtain the consent of the occupants of the premises and subsequent renovations. This article focuses on presenting an opportunity to reduce the number of destructive tests necessary to reliably assess the condition of large-panel structures, which constitute a significant housing stock in Europe. Based on tests carried out on a real building, the risk factors associated with obtaining reliable results by non-destructive methods were determined. Areas where destructive testing is necessary were identified. In addition, reference was made to standard recommendations and guidelines from a reputable research institution. Practical guidelines were formulated regarding the diagnostics of large-panel structures, resulting in a reduction in the number of destructive tests required.
Advances in Electrochemical Energy Storage over Metallic Bismuth-Based Materials
Authors: Cheng Xiaolong , Li Dongjun , Jiang Yu , Huang Fangzhi , Li Shikuo
Published: December 2023

Bismuth (Bi) has been prompted many investigations into the development of next-generation energy storage systems on account of its unique physicochemical properties. Although there are still some challenges, the application of metallic Bi-based materials in the field of energy storage still has good prospects. Herein, we systematically review the application and development of metallic Bi-based anode in lithium ion batteries and beyond-lithium ion batteries. The reaction mechanism, modification methodologies and their relationship with electrochemical performance are discussed in detail. Additionally, owing to the unique physicochemical properties of Bi and Bi-based alloys, some innovative investigations of metallic Bi-based materials in alkali metal anode modification and sulfur cathodes are systematically summarized for the first time. Following the obtained insights, the main unsolved challenges and research directions are pointed out on the research trend and potential applications of the Bi-based materials in various energy storage fields in the future.
Hermitian Solutions of the Quaternion Algebraic Riccati Equations through Zeroing Neural Networks with Application to Quadrotor Control
Authors: Jerbi Houssem , Alshammari Obaid , Aoun Sondess Ben , Kchaou Mourad , Simos Theodore E. , Mourtas Spyridon D. , Katsikis Vasilios N.
Published: December 2023

The stability of nonlinear systems in the control domain has been extensively studied using different versions of the algebraic Riccati equation (ARE). This leads to the focus of this work: the search for the time-varying quaternion ARE (TQARE) Hermitian solution. The zeroing neural network (ZNN) method, which has shown significant success at solving time-varying problems, is used to do this. We present a novel ZNN model called ’ZQ-ARE’ that effectively solves the TQARE by finding only Hermitian solutions. The model works quite effectively, as demonstrated by one application to quadrotor control and three simulation tests. Specifically, in three simulation tests, the ZQ-ARE model finds the TQARE Hermitian solution under various initial conditions, and we also demonstrate that the convergence rate of the solution can be adjusted. Furthermore, we show that adapting the ZQ-ARE solution to the state-dependent Riccati equation (SDRE) technique stabilizes a quadrotor’s flight control system faster than the traditional differential-algebraic Riccati equation solution.
Association of Maternal Age and Blood Markers for Metabolic Disease in Newborns
Authors: Xie Yuhan , Peng Gang , Zhao Hongyu , Scharfe Curt
Published: December 2023

Pregnancy at an advanced maternal age is considered a risk factor for adverse maternal, fetal, and neonatal outcomes. Here we investigated whether maternal age could be associated with differences in the blood levels of newborn screening (NBS) markers for inborn metabolic disorders on the Recommended Universal Screening Panel (RUSP). Population-level NBS data from screen-negative singleton infants were examined, which included blood metabolic markers and covariates such as age at blood collection, birth weight, gestational age, infant sex, parent-reported ethnicity, and maternal age at delivery. Marker levels were compared between maternal age groups (age range: 1544 years) using effect size analyses, which controlled for differences in group sizes and potential confounding from other covariates. We found that 13% of the markers had maternal age-related differences, including newborn metabolites with either increased (Tetradecanoylcarnitine [C14], Palmitoylcarnitine [C16], Stearoylcarnitine [C18], Oleoylcarnitine [C18:1], Malonylcarnitine [C3DC]) or decreased (3-Hydroxyisovalerylcarnitine [C5OH]) levels at an advanced maternal age (≥35 years, absolute Cohen’s d > 0.2). The increased C3DC levels in this group correlated with a higher false-positive rate in newborn screening for malonic acidemia (p-value < 0.001), while no significant difference in screening performance was seen for the other markers. Maternal age is associated with inborn metabolic differences and should be considered together with other clinical variables in genetic disease screening.
Fenpicoxamid-Imprinted Surface Plasmon Resonance (SPR) Sensor Based on Sulfur-Doped Graphitic Carbon Nitride and Its Application to Rice Samples
Authors: Akıcı Şule Yıldırım , Bankoğlu Yola Bahar , Karslıoğlu Betül , Polat İlknur , Atar Necip , Yola Mehmet Lütfi
Published: December 2023

This research attempt involved the development and utilization of a newly designed surface plasmon resonance (SPR) sensor which incorporated sulfur-doped graphitic carbon nitride (S-g-C3N4) as the molecular imprinting material. The primary objective was to employ this sensor for the quantitative analysis of Fenpicoxamid (FEN) in rice samples. The synthesis of S-g-C3N4 with excellent purity was achieved using the thermal poly-condensation approach, which adheres to the principles of green chemistry. Afterwards, UV polymerization was utilized to fabricate a surface plasmon resonance (SPR) chip imprinted with FEN, employing S-g-C3N4 as the substrate material. This process involved the inclusion of N,N′-azobisisobutyronitrile (AIBN) as the initiator, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, methacryloylamidoglutamic acid (MAGA) as the monomer, and FEN as the analyte. After successful structural analysis investigations on a surface plasmon resonance (SPR) chip utilizing S-g-C3N4, which was imprinted with FEN, a comprehensive investigation was conducted using spectroscopic, microscopic, and electrochemical techniques. Subsequently, the kinetic analysis applications, namely the determination of the limit of quantification (LOQ) and the limit of detection (LOD), were carried out. For analytical results, the linearity of the FEN-imprinted SPR chip based on S-g-C3N4 was determined as 1.0–10.0 ng L−1 FEN, and LOQ and LOD values were obtained as 1.0 ng L−1 and 0.30 ng L−1, respectively. Finally, the prepared SPR sensor’s high selectivity, repeatability, reproducibility, and stability will ensure safe food consumption worldwide.
Array Study of VLF Thin-Film Magnetoelectric Antenna with a Microbridge Structure
Authors: Jin Jianhua , Jing Long , Zuo Chao , Ma Zhiling , Shi Yanfeng , Yang Xiaofei , Chen Shi
Published: December 2023

Recently, magnetoelectric (ME) antennas have become a hot topic in the field of antenna miniaturization in the very-low-frequency (VLF) band because their size can be reduced to one-ten-thousandth of the size of conventional electric antennas. However, they still suffer from narrow transmission/reception bandwidth and weak radiation intensity. To address these issues, VLF thin-film ME antennas with a microbridge structure are designed, and the method of array connection is used. Test results show that the detection limit of the ME antenna unit is 636 pT/√Hz at 23 kHz and the radiant magnetic field intensity at 0.12 m is 0.87 nT (input power of 10 mW). By series-connecting three ME antenna units with the same resonance frequency, the output response has been increased to 1.72 times and the EM wave radiation intensity is increased to 1.9 times compared to a single unit. By parallel-connecting two ME antenna units with different resonance frequencies, the output response bandwidth has been expanded to 1.56 times compared to a single unit, and the signal radiation bandwidth has been expanded to 1.47 times. This work provides a valuable reference for the future larger-scale arraying of ME antennas.
Combining Electrical Resistivity, Induced Polarization, and Self-Potential for a Better Detection of Ore Bodies
Authors: Su Zhaoyang , Revil André , Ghorbani Ahmad , Zhang Xin , Zhao Xiang , Richard Jessy
Published: December 2023

Electrical resistivity (ER), induced polarization (IP), and self-potential (SP) are three geophysical methods that have been broadly used in the realm of mineral exploration. These geophysical methods provide complementary information, each exhibiting a distinct sensitivity to various types of mineral deposits. Considering the relationship among these three methods, we propose an integrated approach that merges their respective information to offer an improved localization technique for ore bodies. First, we invert the electrical conductivity distribution through electrical resistance tomography (ERT). Then, we use the inverted conductivity distribution to invert the IP and SP data in terms of chargeability and source current density distributions. Then, we normalize the inverted chargeability and source current density distributions and we combine them to obtain an ore body index (ORI) χ used to delineate the potential locations of ore deposits. We design this index to be sensitive to the presence of ore bodies, which are reflected by either strong and localized source current density (SP) and/or strong chargeability values (IP). The proposed method is first validated using a synthetic model with two distinct anomalies characterized by different properties. The results show the limitation of individual inversion, as each method exclusively detects one of these anomalies. The combined approach allows a better characterization of the target. Then, the approach is applied to a sandbox experiment in which two metallic bodies are buried in water-saturated sand used as the background. Again, the proposed methodology is successfully applied to the detection of the metallic targets, improving their localization compared with individual methods.
Isoprenylated Flavonoids and 2-Arylbenzofurans from the Root Bark of Morus alba L. and Their Cytotoxic Activity against HGC27 Cancer Cells
Authors: Pu Hangyi , Cao Dongyi , Zhou Xue , Li Fu , Wang Lun , Wang Mingkui
Published: December 2023

Three new compounds (1, 11, and 12), together with 32 known ones, were isolated from the root bark of Morus alba L. using various chromatographic methods. The structures of the undescribed compounds were elucidated based on 1D, 2D NMR, and HRESIMS dataanalysis, while the known ones were identified by comparison of their spectroscopic data with those reported in the literature. All the isolates were evaluated for their cytotoxic activities against human gastric cancer HGC27 cells by CCK-8 assay. Among them, compounds 5, 8, 10, and 30 exhibited cytotoxic activities on HGC27 cells with IC50 values of 33.76 ± 2.64 μM, 28.94 ± 0.72 μM, 6.08 ± 0.34 μM, and 10.24 ± 0.89 μM, respectively. Furthermore, compound 10 was confirmed to reduce proliferation ability, increase apoptosis rate, and inhibit cell migration pathway by annexin V/PI double staining experiment, transwell experiment, and Western blot analysis.
Optimized DOX Drug Deliveries via Chitosan-Mediated Nanoparticles and Stimuli Responses in Cancer Chemotherapy: A Review
Authors: Imran HafizMuhammad , Tang Yixin , Wang Siyuan , Yan Xiuzhang , Liu Chang , Guo Lei , Wang Erlei , Xu Caina
Published: December 2023

Chitosan nanoparticles (NPs) serve as useful multidrug delivery carriers in cancer chemotherapy. Chitosan has considerable potential in drug delivery systems (DDSs) for targeting tumor cells. Doxorubicin (DOX) has limited application due to its resistance and lack of specificity. Chitosan NPs have been used for DOX delivery because of their biocompatibility, biodegradability, drug encapsulation efficiency, and target specificity. In this review, various types of chitosan derivatives are discussed in DDSs to enhance the effectiveness of cancer treatments. Modified chitosan–DOX NP drug deliveries with other compounds also increase the penetration and efficiency of DOX against tumor cells. We also highlight the endogenous stimuli (pH, redox, enzyme) and exogenous stimuli (light, magnetic, ultrasound), and their positive effect on DOX drug delivery via chitosan NPs. Our study sheds light on the importance of chitosan NPs for DOX drug delivery in cancer treatment and may inspire the development of more effective approaches for cancer chemotherapy.
The Synthesis and Properties of Ladder-Type π-Conjugated Compounds with Pyrrole and Phosphole Rings
Authors: Truong Minh Anh , Morishita Suzuho , Noguchi Keiichi , Nakano Koji
Published: December 2023

The phosphole ring is known as a useful building block for constructing π-conjugated organic materials. Here, we report ladder-type benzophospholo[3,2-b]indole (BPI) derivatives where the phosphole and the pyrrole rings are directly fused. Compounds 8a–8d with different aryl groups on the phosphorous center were successfully synthesized, and the solid-state structure of 8a was confirmed using X-ray crystallographic analysis. The BPIs exhibit relatively high fluorescence quantum yield (Φ 0.50−0.72) and demonstrate a larger Stokes shift compared with a series of benzophospholo[3,2-b]benzoheteroles. The benzophospholo[3,2-b]carbazole derivative 9, which possesses a benzene ring between the phosphole and the pyrrole rings of the BPI, was also synthesized, and its solid-state structure was confirmed using X-ray crystallographic analysis. Compound 9 was found to show a smaller Stokes shift compared with the BPI.
Ethnomedicinal Uses, Geographical Distribution, Botanical Description, Phytochemistry, Pharmacology, and Quality Control of Laportea bulbifera (Sieb. et Zucc.) Wedd.: A Review
Authors: Feng Jiaxin , Xia Guangqing , Zhu Junyi , Li Li , Zang Hao
Published: December 2023

Laportea bulbifera (Sieb. et Zucc.) Wedd. (L. bulbifera) is a significant plant in the Laportea genus. Traditionally, it has been used in ethnomedicine for treating various conditions such as rheumatic arthralgia, fractures, falling injuries, nephritis dropsy, limb numbness, pruritus, fatigue-induced internal imbalances, and irregular menstruation. Modern pharmacological studies have confirmed its therapeutic potential, including anti-inflammatory, immunosuppressive, analgesic, and anti-rheumatoid arthritis properties. To gather comprehensive information on L. bulbifera, a thorough literature search was conducted using databases like Web of Science, PubMed, ProQuest, and CNKI. This review aims to provide a comprehensive understanding of L. bulbifera, covering various aspects such as ethnomedicinal uses, geographical distribution, botanical description, phytochemistry, pharmacology, and quality control. The goal is to establish a solid foundation and propose new research avenues for exploring and developing potential applications of L. bulbifera. So far, a total of one hundred and eighty-nine compounds have been isolated and identified from L. bulbifera, including flavonoids, phenolics, nitrogen compounds, steroids, terpenoids, coumarins, phenylpropanoids, fatty acids and their derivatives, and other compounds. Notably, flavonoids and fatty acids have demonstrated remarkable antioxidant and anti-inflammatory properties. Additionally, these compounds show promising potential in activities such as analgesia, hypoglycemia, and hypolipidemia, as well as toxicity. Despite extensive fundamental studies on L. bulbifera, further research is still needed to enhance our understanding of its mechanism of action and improve quality control. This requires more comprehensive investigations to explore the specific material basis, uncover new mechanisms of action, and refine quality control methods related to L. bulbifera. By doing so, we could contribute to the further development and utilization of this plant.
Air- and Water-Stable Heteroleptic Copper (I) Complexes Bearing Bis(indazol-1-yl)methane Ligands: Synthesis, Characterisation, and Computational Studies
Authors: Moreno-da Costa David , Zúñiga-Loyola César , Droghetti Federico , Robles Stephania , Villegas-Menares Alondra , Villegas-Escobar Nery , Gonzalez-Pavez Ivan , Molins Elies , Natali Mirco , Cabrera Alan R.
Published: December 2023

A series of four novel heteroleptic Cu(I) complexes, bearing bis(1H-indazol-1-yl)methane analogues as N,N ligands and DPEPhos as the P,P ligand, were synthesised in high yields under mild conditions and characterised by spectroscopic and spectrometric techniques. In addition, the position of the carboxymethyl substituent in the complexes and its effect on the electrochemical and photophysical behaviour was evaluated. As expected, the homoleptic copper (I) complexes with the N,N ligands showed air instability. In contrast, the obtained heteroleptic complexes were air- and water-stable in solid and solution. All complexes displayed green-yellow luminescence in CH2Cl2 at room temperature due to ligand-centred (LC) phosphorescence in the case of the Cu(I) complex with an unsubstituted N,N ligand and metal-to-ligand charge transfer (MLCT) phosphorescence for the carboxymethyl-substituted complexes. Interestingly, proper substitution of the bis(1H-indazol-1-yl)methane ligand enabled the achievement of a remarkable luminescent yield (2.5%) in solution, showcasing the great potential of this novel class of copper(I) complexes for potential applications in luminescent devices and/or photocatalysis.
A Non-Matrix-Matched Calibration Method for In Situ Major and Trace Element Analysis of Scheelite by Nanosecond LA-ICP-MS
Authors: Tan Xijuan , Tian Honghao , Lu Lin , Xiong Dongyang , Liang Ting
Published: December 2023

In this work, a reliable and robust in situ non-matrix-matched calibration method is proposed for element composition determination in scheelite samples. With external calibration against the silicate glass standard reference material NIST SRM 610, the concentrations of both major elements (Ca and W) and trace elements (Si, Fe, Mo, Y, rare earth elements, etc.) in scheelite are determined using an ArF 193 nm excimer nanosecond laser ablation-inductively coupled plasma mass spectrometer (LA-ICP-MS). Here, the ablation was performed by hole drilling under a helium (He) environment using a laser spot size of 35 μm and a laser repetition of 5 Hz, and the aerosols were then transported to a quadrupole ICP-MS by a mixture of He and make-up gas argon (Ar) with a total gas flow rate of 1.6 L/min. Results showed that there was no apparent matrix effect between the NIST SRM 610 and scheelite by this proposed method. With internal standardization against W, the obtained concentrations of CaO and WO3 were found to yield an average matrix CaO/WO3 mass fraction ratio of 0.245 (2σ = 0.003, n = 19), which agreed well with the value of 0.243 (2σ = 0.002, n = 15) from electron probe microanalysis (EPMA). Furthermore, the accuracy of trace element analyses with this proposed non-matrix-matched calibration in situ method was evaluated by comparing the concentration results with those from bulk analysis by solution nebulizer ICP-MS (SN-ICP-MS). It was found that the quantification results from LA-ICP-MS and SN-ICP-MS were comparable, in particular showing a relative concentration bias of the total ∑REE+Y contents of less than 2%. This confirmed that scheelites can be accurately analyzed in situ by LA-ICP-MS without matrix-matched calibration standards. By using this developed in situ method, the element compositions in a series of scheelite samples from different W-associated deposits in China were successfully quantified, promising further genetic process investigation and associated geologic activities of the polymetallic resources.
Tailored Porous Carbon Xerogels for Fe-N-C Catalysts in Proton Exchange Membrane Fuel Cells
Authors: Álvarez-Manuel Laura , Alegre Cinthia , Sebastián David , Napal Pedro F. , Lázaro María Jesús
Published: December 2023

Atomically dispersed Fe-N-C catalysts for the oxygen reduction reaction (ORR) have been synthesized with a template-free method using carbon xerogels (CXG) as a porous matrix. The porosity of the CXGs is easily tunable through slight variations in the synthesis procedure. In this work, CXGs are prepared by formaldehyde and resorcinol polymerization, modifying the pH during the process. Materials with a broad range of porous structures are obtained: from non-porous to micro-/meso-/macroporous materials. The porous properties of CXG have a direct effect on Fe-N-CXG activity against ORR in an acidic medium (0.5 M H2SO4). Macropores and wide mesopores are vital to favor the mass transport of reagents to the active sites available in the micropores, while narrower mesopores can generate additional tortuosity. The role of microporosity is investigated by comparing two Fe-N-C catalysts using the same CXG as the matrix but following a different Fe and N doping procedure. In one case, the carbonization of CXG occurs rapidly and simultaneously with Fe and N doping, whereas in the other case it proceeds slowly, under controlled conditions and before the doping process, resulting in the formation of more micropores and active sites and achieving higher activity in a three-electrode cell and a better durability during fuel cell measurements. This work proves the feasibility of the template-free method using CXG as a carbon matrix for Fe-N-C catalysts, with the novelty of the controlled porous properties of the carbon material and its effect on the catalytic activity of the Fe-N-C catalyst. Moreover, the results obtained highlight the importance of the carbon matrix’s porous structure in influencing the activity of Fe-N-C catalysts against ORR.
Resonance-Based Sensing of Magnetic Nanoparticles Using Microfluidic Devices with Ferromagnetic Antidot Nanostructures
Authors: Dowling Reyne , Narkowicz Ryszard , Lenz Kilian , Oelschlägel Antje , Lindner Jürgen , Kostylev Mikhail
Published: December 2023

We demonstrated resonance-based detection of magnetic nanoparticles employing novel designs based upon planar (on-chip) microresonators that may serve as alternatives to conventional magnetoresistive magnetic nanoparticle detectors. We detected 130 nm sized magnetic nanoparticle clusters immobilized on sensor surfaces after flowing through PDMS microfluidic channels molded using a 3D printed mold. Two detection schemes were investigated: (i) indirect detection incorporating ferromagnetic antidot nanostructures within microresonators, and (ii) direct detection of nanoparticles without an antidot lattice. Using scheme (i), magnetic nanoparticles noticeably downshifted the resonance fields of an antidot nanostructure by up to 207 G. In a similar antidot device in which nanoparticles were introduced via droplets rather than a microfluidic channel, the largest shift was only 44 G with a sensitivity of 7.57 G/ng. This indicated that introduction of the nanoparticles via microfluidics results in stronger responses from the ferromagnetic resonances. The results for both devices demonstrated that ferromagnetic antidot nanostructures incorporated within planar microresonators can detect nanoparticles captured from dispersions. Using detection scheme (ii), without the antidot array, we observed a strong resonance within the nanoparticles. The resonance’s strength suggests that direct detection is more sensitive to magnetic nanoparticles than indirect detection using a nanostructure, in addition to being much simpler.
Fulminant Pneumonia Due to Reactivation of Latent Toxoplasmosis in a Cat—A Case Report
Authors: Fietz Simone A. , Grochow Thomas , Schares Gereon , Töpfer Tanja , Heilmann Romy M.
Published: December 2023

Toxoplasma (T.) gondii is an obligate intracellular parasite with felids, including domestic cats, as definitive hosts. In immunocompetent individuals, T. gondii infection is usually asymptomatic. However, under immunosuppression, it may have severe pathological impacts, which often result from the reactivation of a chronic infection. In this case study, a 21-month-old female domestic shorthair cat—diagnosed with primary immune-mediated hemolytic anemia three months prior and treated with cyclosporine and prednisolone—presented with acute tachypnea, dyspnea, diarrhea, and anorexia. Thoracic radiography suggested severe pneumonia. Testing for Mycoplasma spp., Anaplasma spp., Ehrlichia spp., and lungworm infection was negative. Serology for T. gondii revealed seroconversion of IgG, but not of IgM, indicating previous exposure to T. gondii. The cat remained stable but tachypneic for three days, followed by an acute onset of dyspnea and clinical deterioration, after which euthanasia was elected. Numerous protozoa were present in a postmortem transtracheal bronchoalveolar lavage and fine-needle aspiration of the lung. Microsatellite typing classified the extracted DNA as T. gondii type II variant TgM-A. This case demonstrates that T. gondii reactivation, leading to fulminant pneumonia, can be a sequela of immunosuppressive treatment in cats and should, therefore, be considered as a differential diagnosis in immunosuppressed cats with acute-onset respiratory signs. Rapid diagnosis may prevent fatal consequences.
Population Pharmacokinetic Modeling for Twice-Daily Intravenous Busulfan in a Large Cohort of Pediatric Patients Undergoing Hematopoietic Stem Cell Transplantation—A 10-Year Single-Center Experience
Authors: Schreib Katharina M. , Bräm Dominic S. , Zeilhofer Ulrike Barbara , Müller Daniel , Güngör Tayfun , Krämer Stefanie D. , Hauri-Hohl Mathias M.
Published: December 2023

Reaching target exposure of busulfan-based conditioning prior to hematopoietic stem cell transplantation is vital for favorable therapy outcomes. Yet, a wide inter-patient and inter-occasion variability in busulfan exposure has been reported, especially in children. We aimed to identify factors associated with the variability of busulfan pharmacokinetics in 124 consecutive patients transplanted at the University Children’s Hospital Zurich between October 2010 and February 2020. Clinical data and busulfan plasma levels after twice-daily intravenous administration were analyzed retrospectively by population pharmacokinetic modeling. The volume of distribution correlated with total body water. The elimination rate constant followed an age-dependent maturation function, as previously suggested, and correlated with the levels of serum albumin. Acute lymphoblastic leukemia reduced busulfan clearance by 20%. Clearance significantly decreased by 17% on average from the start to the third day of busulfan administration, in agreement with other studies. An average reduction of 31% was found in patients with hemophagocytic lymphohistiocytosis and X-linked lymphoproliferative disease. In conclusion, we demonstrate that in addition to known factors, underlying disease and serum albumin significantly impact busulfan pharmacokinetics in pediatric patients; yet, substantial unexplained variability in some patients remained. Thus, we consider repeated pharmacokinetic assessment essential to achieve the desired target exposure in twice-daily busulfan administration.
Recent Innovations of Mesoporous Silica Nanoparticles Combined with Photodynamic Therapy for Improving Cancer Treatment
Authors: Nady Doaa , Hassan Afnan , Amin Muhammad , Bakowsky Udo , Fahmy Sherif
Published: December 2023

Cancer is a global health burden and is one of the leading causes of death. Photodynamic therapy (PDT) is considered an alternative approach to conventional cancer treatment. PDT utilizes a light-sensitive compound, photosensitizers (PSs), light irradiation, and molecular oxygen (O2). This generates cytotoxic reactive oxygen species (ROS), which can trigger necrosis and/ or apoptosis, leading to cancer cell death in the intended tissues. Classical photosensitizers impose limitations that hinder their clinical applications, such as long-term skin photosensitivity, hydrophobic nature, nonspecific targeting, and toxic cumulative effects. Thus, nanotechnology emerged as an unorthodox solution for improving the hydrophilicity and targeting efficiency of PSs. Among nanocarriers, mesoporous silica nanoparticles (MSNs) have gained increasing attention due to their high surface area, defined pore size and structure, ease of surface modification, stable aqueous dispersions, good biocompatibility, and optical transparency, which are vital for PDT. The advancement of integrated MSNs/PDT has led to an inspiring multimodal nanosystem for effectively treating malignancies. This review gives an overview of the main components and mechanisms of the PDT process, the effect of PDT on tumor cells, and the most recent studies that reported the benefits of incorporating PSs into silica nanoparticles and integration with PDT against different cancer cells.
Identification of Quantitative Trait Nucleotides and Development of Diagnostic Markers for Nine Fatty Acids in the Peanut
Authors: Wang Juan , Chen Haoning , Li Yuan , Shi Dachuan , Wang Wenjiao , Yan Caixia , Yuan Mei , Sun Quanxi , Chen Jing , Mou Yifei , Qu Chunjuan , Shan Shihua
Published: December 2023

The cultivated peanut (Arachis hypogaea L.) is an important oilseed crop worldwide, and fatty acid composition is a major determinant of peanut oil quality. In the present study, we conducted a genome-wide association study (GWAS) for nine fatty acid traits using the whole genome sequences of 160 representative Chinese peanut landraces and identified 6-1195 significant SNPs for different fatty acid contents. Particularly for oleic acid and linoleic acid, two peak SNP clusters on Arahy.09 and Arahy.19 were found to contain the majority of the significant SNPs associated with these two fatty acids. Additionally, a significant proportion of the candidate genes identified on Arahy.09 overlap with those identified in early studies, among which three candidate genes are of special interest. One possesses a significant missense SNP and encodes a known candidate gene FAD2A. The second gene is the gene closest to the most significant SNP for linoleic acid. It codes for an MYB protein that has been demonstrated to impact fatty acid biosynthesis in Arabidopsis. The third gene harbors a missense SNP and encodes a JmjC domain-containing protein. The significant phenotypic difference in the oleic acid/linoleic acid between the genotypes at the first and third candidate genes was further confirmed with PARMS analysis. In addition, we have also identified different candidate genes (i.e., Arahy.ZV39IJ, Arahy.F9E3EA, Arahy.X9ZZC1, and Arahy.Z0ELT9) for the remaining fatty acids. Our findings can help us gain a better understanding of the genetic foundation of peanut fatty acid contents and may hold great potential for enhancing peanut quality in the future.
Bioactive Compounds in Moringa oleifera: Mechanisms of Action, Focus on Their Anti-Inflammatory Properties
Authors: Chiș Adina , Noubissi Paul Aimé , Pop Oana-Lelia , Mureșan Carmen Ioana , Fokam Tagne Michel Archange , Kamgang René , Fodor Adriana , Sitar-Tăut Adela-Viviana , Cozma Angela , Orășan Olga Hilda , Hegheș Simona Codruța , Vulturar Romana , Suharoschi Ramona
Published: December 2023

Moringa oleifera (M. oleifera) is a tropical tree native to Pakistan, India, Bangladesh, and Afghanistan; it is cultivated for its nutritious leaves, pods, and seeds. This scientific study was conducted to outline the anti-inflammatory properties and mechanisms of action of bioactive compounds from M. oleifera. The existing research has found that the plant is used in traditional medicine due to its bioactive compounds, including phytochemicals: flavonoids and polyphenols. The compounds are thought to exert their anti-inflammatory effects due to: (1) inhibition of pro-inflammatory enzymes: quercetin and kaempferol inhibit the pro-inflammatory enzymes (cyclooxygenase and lipoxygenase); (2) regulation of cytokine production: isothiocyanates modulate signaling pathways involved in inflammation, such as the nuclear factor-kappa B (NF-kappa B) pathway; isothiocyanates inhibit the production of pro-inflammatory cytokines such as TNF-α (tumor necrosis factor α) and IL-1β (interleukin-1β); and (3) antioxidant activity: M. oleifera contains flavonoids, polyphenols, known to reduce oxidative stress and inflammation. The review includes M. oleifera’s effects on cardiovascular protection, anti-hypertensive activities, type 2 diabetes, inflammatory bowel disease, and non-alcoholic fatty liver disease (NAFLD). This research could prove valuable for exploring the pharmacological potential of M. oleifera and contributing to the prospects of developing effective medicines for the benefit of human health.
Functional Analysis of PbbZIP11 Transcription Factor in Response to Cold Stress in Arabidopsis and Pear
Authors: Zhang Yuxin , Wu Lin , Liu Lun , Jia Bing , Ye Zhenfeng , Tang Xiaomei , Heng Wei , Liu Li
Published: December 2023

Cold stress is a prominent abiotic factor that adversely affects the growth and yield of pears, consequently restricting the cultivation range and resulting in substantial economic losses for the pear industry. Basic region–leucine zipper (bZIP) transcription factors are widely involved in multiple physiological and biochemical activities of plants, particularly in response to cold stress. In this study, the responsiveness of PbbZIP11 in pear to cold stress was investigated, and its role was explored by using pear callus and Arabidopsis thaliana. The findings revealed that overexpression of PbbZIP11 enhanced the tolerance of pear callus and Arabidopsis thaliana to cold stress. The antioxidant enzyme activities of transgenic plants were enhanced and the expression of C-repeat binding transcription factor (CBF) genes was increased as compared to wild-type plants. To better understand the biological function of PbbZIP11, mRNAs were isolated from overexpressed and wild-type Arabidopsis thaliana after cold stress for whole-genome sequencing. The results showed that the expression of some CBF downstream target genes changed after exposure to cold stress. The results suggested that the PbbZIP11 gene could participate in cold-stress signaling through the CBF-dependent pathway, which provides a theoretical basis for the PbbZIP11-mediated response to cold stress and for the genetic breeding of pear varieties with low-temperature tolerance.
Influence of Post-Processing on the Degree of Conversion and Mechanical Properties of 3D-Printed Polyurethane Aligners
Authors: Šimunović Luka , Jurela Antonija , Sudarević Karlo , Bačić Ivana , Haramina Tatjana , Meštrović Senka
Published: December 2023

Background: This study explores how different post-processing methods affect the mechanical properties and degree of conversion of 3d-printed polyurethane aligners made from Tera Harz TC-85 resin. Methods: Using Fourier-transform infrared (FTIR) spectroscopy, the degree of conversion of liquid resin and post-processed materials was analyzed. This investigation focused on the effects of various post-curing environments (nitrogen vs. air) and rinsing protocols (centrifuge, ethanol, isopropanol, and isopropanol + water). The assessed mechanical properties were flexural modulus and hardness. Results: The degree of conversion showed no significant variance across different groups, though the polymerization environment influenced the results, accounting for 24.0% of the variance. The flexural modulus varied considerably, depending on both the rinsing protocol and the polymerization environment. The standard protocol (centrifugation followed by nitrogen polymerization) exhibited the highest flexural modulus of 1881.22 MPa. Hardness testing revealed significant differences, with isopropanol treatments showing increased resistance to wear in comparison to the centrifuge and ethanol rinse treatments. Conclusions: This study conclusively demonstrates the adverse effects of oxygen on the polymerization process, underscoring the critical need for an oxygen-free environment to optimize material properties. Notably, the ethanol rinse followed by nitrogen polymerization protocol emerged as a viable alternative to the conventional centrifuge plus nitrogen method.