bluuebunny/crossref_metadata_2025 huggingface dataset:
Long-Term Nightshift Work and Breast Cancer Risk: An Updated Systematic Review and Meta-Analysis with Special Attention to Menopausal Status and to Recent Nightshift Work
Authors: Schwarz Christine , Pedraza-Flechas Ana María , Pastor-Barriuso Roberto , Lope Virginia , de Larrea Nerea Fernández , Jiménez-Moleón José Juan , Pollán Marina , Pérez-Gómez Beatriz
Published: November 2021

This systematic review discusses long-term NSW and female BC risk, with special attention to differences between pre- and postmenopausal BC, to test the association with recent NSW. The review follows PRISMA guidelines (Prospero registry: CRD42018102515). We searched PubMed, Embase, and WOS for case–control, nested case–control, and cohort studies addressing long-term NSW (≥15 years) as risk exposure and female BC as outcome until 31 December 2020. Risk of bias was evaluated with the Newcastle–Ottawa scale. Eighteen studies were finally included (eight cohorts; five nested case–control; five case–control). We performed meta-analyses on long-term NSW and BC risk; overall and by menopausal status; a subanalysis on recent long-term NSW, based on studies involving predominantly women below retirement age; and a dose–response meta-analysis on NSW duration. The pooled estimate for long-term NSW and BC was 1.13 (95%CI = 1.01–1.27; 18 studies, I2 = 56.8%, p = 0.002). BC risk increased 4.7% per 10 years of NSW (95%CI = 0.94–1.09; 16 studies, I2 = 33.4%, p = 0.008). The pooled estimate for premenopausal BC was 1.27 (95%CI = 0.96–1.68; six studies, I2 = 32.0%, p = 0.196) and for postmenopausal BC 1.05 (95%CI = 0.90–1.24,I2 = 52.4%; seven studies, p = 0.050). For recent long-term exposure, the pooled estimate was 1.23 (95%CI = 1.06–1.42; 15 studies; I2 = 48.4%, p = 0.018). Our results indicate that long-term NSW increases the risk for BC and that menopausal status and time since exposure might be relevant.
A Patient-Derived Xenograft Model of Dedifferentiated Endometrial Carcinoma: A Proof-of-Concept Study for the Identification of New Molecularly Informed Treatment Approaches
Authors: Lin Chiao-Yun , Wu Ren-Chin , Huang Chen-Yang , Lai Chyong-Huey , Chao An-Shine , Li Hsin-Pai , Tsai Chia-Lung , Kuek Elizabeth Joo-Wen , Hsu Cheng-Lung , Chao Angel
Published: November 2021

Conventional treatment of dedifferentiated endometrial carcinoma (DEC)–an uncommon and highly aggressive uterine malignancy–is beset by high failure rates. A line of research that holds promise to overcome these limitations is tailored treatments targeted on specific molecular alterations. However, suitable preclinical platforms to allow a reliable implementation of this approach are still lacking. Here, we developed a patient-derived xenograft (PDX) model for preclinical testing of investigational drugs informed by molecular data. The model–termed PDX-mLung was established in mice implanted with lung metastatic lesions obtained from a patient with DEC. Histologic and whole-exome genetic analyses revealed a high degree of identity between PDX-mLung and the patient’s parental lesions (both primary DEC and lung metastases). Interestingly, molecular analyses revealed that PDX-mLung harbored druggable alterations including a FGFR2 mutation and CCNE2 amplification. Targeted combined treatment with the FGFR inhibitor lenvatinib and the cell cycle inhibitor palbociclib was found to exert synergistic therapeutic effects against in vivo tumor growth. Based on the results of RNA sequencing, lenvatinib and palbociclib were found to exert anti-tumor effects by interfering interferon signaling and activating hormonal pathways, respectively. Collectively, these data provide proof-of-concept evidence on the value of PDX models for preclinical testing of molecularly informed drug therapy in difficult-to-treat human malignancies. Further clinical research is needed to examine more rigorously the potential usefulness of the lenvatinib and palbociclib combination in patients with DEC.
Gp-100 as a Novel Therapeutic Target in Uveal Melanoma
Authors: Martinez-Perez Daniel , Viñal David , Solares Isabel , Espinosa Enrique , Feliu Jaime
Published: November 2021

Uveal melanoma is a rare neoplasm with poor prognosis in the metastatic setting. Unlike cutaneous melanoma, treatment with kinase inhibitors or immune checkpoint inhibitors is not effective. Glycoprotein 100 (Gp-100) is a protein highly expressed in melanocytes and melanoma that has recently been effectively targeted by tebentafusp, a first-in-class bispecific protein of the immune-mobilizing monoclonal T cell receptors against cancer (ImmTACs) family. Tebentafusp targets tumor cells that express a peptide of Gp-100 presented by HLA*A0201, creating an immune synapse that kills targeted tumor cells. Recently, a randomized phase III trial reported an overall survival benefit for tebentafusp in patients with untreated metastatic uveal melanoma. The aim of this comprehensive review is to summarize evidence of Gp-100 as a therapeutic target in melanoma, and the preclinical and clinical development of tebentafusp as a novel therapeutic strategy for patients with uveal melanoma.
Clinical Biomarkers for Early Identification of Patients with Intracranial Metastatic Disease
Authors: Gaebe Karolina , Li Alyssa Y. , Das Sunit
Published: November 2021

Nearly 30% of patients with cancer will develop intracranial metastatic disease (IMD), and more than half of these patients will die within a few months following their diagnosis. In light of the profound effect of IMD on survival and quality of life, there is significant interest in identifying biomarkers that could facilitate the early detection of IMD or identify patients with cancer who are at high IMD risk. In this review, we will highlight early efforts to identify biomarkers of IMD and consider avenues for future investigation.
MiR-195 and Its Target SEMA6D Regulate Chemoresponse in Breast Cancer
Authors: Baxter Diana E. , Allinson Lisa M. , Al Amri Waleed S. , Poulter James A. , Pramanik Arindam , Thorne James L. , Verghese Eldo T. , Hughes Thomas A.
Published: November 2021

Background: poor prognosis primary breast cancers are typically treated with cytotoxic chemotherapy. However, recurrences remain relatively common even after this aggressive therapy. Comparison of matched tumours pre- and post-chemotherapy can allow identification of molecular characteristics of therapy resistance and thereby potentially aid discovery of novel predictive markers or targets for chemosensitisation. Through this comparison, we aimed to identify microRNAs associated with chemoresistance, define microRNA target genes, and assess targets as predictors of chemotherapy response. Methods: cancer cells were laser microdissected from matched breast cancer tissues pre- and post-chemotherapy from estrogen receptor positive/HER2 negative breast cancers showing partial responses to epirubicin/cyclophosphamide chemotherapy (n = 5). MicroRNA expression was profiled using qPCR arrays. MicroRNA/mRNA expression was manipulated in estrogen receptor positive/HER2 negative breast cancer cell lines (MCF7 and MDA-MB-175 cells) with mimics, inhibitors or siRNAs, and chemoresponse was assessed using MTT and colony forming survival assays. MicroRNA targets were identified by RNA-sequencing of microRNA mimic pull-downs, and comparison of these with mRNAs containing predicted microRNA binding sites. Survival correlations were tested using the METABRIC expression dataset (n = 1979). Results: miR-195 and miR-26b were consistently up-regulated after therapy, and changes in their expression in cell lines caused significant differences in chemotherapy sensitivity, in accordance with up-regulation driving resistance. SEMA6D was defined and confirmed as a target of the microRNAs. Reduced SEMA6D expression was significantly associated with chemoresistance, in accordance with SEMA6D being a down-stream effector of the microRNAs. Finally, low SEMA6D expression in breast cancers was significantly associated with poor survival after chemotherapy, but not after other therapies. Conclusions: microRNAs and their targets influence chemoresponse, allowing the identification of SEMA6D as a predictive marker for chemotherapy response that could be used to direct therapy or as a target in chemosensitisation strategies.
Higher Accumulation of Visceral Adipose Tissue Is an Independent Risk Factor for Hepatocellular Carcinoma among Viral Hepatitis Patients with Non-Cirrhotic Livers
Authors: Imai Kenji , Takai Koji , Miwa Takao , Maeda Toshihide , Hanai Tatsunori , Shirakami Yohei , Suetsugu Atsushi , Shimizu Masahito
Published: November 2021

This study aimed to determine the risk factors for hepatocellular carcinoma in non-cirrhotic livers among viral hepatitis patients. A total of 333 HCC cases, including 69 hepatitis B virus (HBV)-related and 264 hepatitis C virus (HCV)-related, were divided into cirrhotic (Fibrosis-4 [FIB-4] index > 3.25) and non-cirrhotic groups (FIB-4 index ≤ 3.25). The clinical characteristics of the two groups were compared. The independent risk factors for the development of HCC were analyzed using logistic regression analysis. The patients with HBV-related HCC were significantly younger, had better Child-Pugh scores, lower FIB-4 index and Mac-2 binding protein glycosylated isomers (M2BPGi) levels, more progressive cancer stage, and higher alpha-fetoprotein (AFP) levels than those with HCV-related HCC. Diabetes mellitus and hypertension were less common in patients with HBV-related HCC. The non-cirrhotic group with HBV-related HCC had a higher visceral adipose tissue index (VATI), better Child-Pugh score, and higher hemoglobin A1c (HbA1c), whereas the one with HCV-related HCC had a higher proportion of men, higher VATI, better Child-Pugh score, higher HbA1c, and a higher prevalence of hypertension, than the corresponding cirrhotic groups. Logistic regression analyses demonstrated that age, male sex, VATI, HbA1c, the presence of hypertension, and HBV etiology were independent risk factors for HCC in a non-cirrhotic liver. A high accumulation of VAT is a risk factor for HCC in patients with non-cirrhotic livers.
Pain Behavioural Response to Acoustic and Light Environmental Changes in Very Preterm Infants
Authors: Marchal Audrey , Melchior Meggane , Dufour André , Poisbeau Pierrick , Zores Claire , Kuhn Pierre
Published: November 2021

Noise and high light illumination in the neonatal intensive care unit (NICU) are recognized as stressors that could alter the well-being and development of vulnerable preterm infants. This prospective observational study evaluated the pain behaviours of very preterm infants (VPIs) to sound peaks (SPs) and light levels variations (LLVs) in the NICU. We measured spontaneously occurring SPs and LLVs in the incubators of 26 VPIs over 10 h. Their behavioural responses were analysed through video recordings using the “Douleur Aigue du Nouveau-né” (DAN) scale. We compared the maximum DAN scores before and after environmental stimuli and the percentage of VPIs with a score ≥ 3 according to the type of stimuli. A total of 591 SPs and 278 LLVs were analysed. SPs of 5 to 15 dBA and LLVs significantly increased the maximum DAN scores compared to baseline. The occurrence of DAN scores ≥ 3 increased with both stressors, with a total of 16% of SPs and 8% of LLVs leading to quantifiable pain behaviour. Altogether, this study shows that VPIs are sensitive to SPs and LLVs, with a slighter higher sensitivity to SPs. The mechanisms leading to pain behaviours induced by noise and light changes should be evaluated further in the context of VPIs brain development. Our results provide further arguments to optimize the NICU sensory environment of neonatal units and to adapt it to the expectations and sensory abilities of VPIs.
Botulinum Toxin Injections for Treatment of Drooling in Children with Cerebral Palsy: A Systematic Review and Meta-Analysis
Authors: Hung Shang-An , Liao Chung-Lun , Lin Wei-Pin , Hsu Jason C. , Guo Yao-Hong , Lin Yu-Ching
Published: November 2021

Background: We aimed to review and analyse the effectiveness and safety of botulinum toxin type A (BoNT-A) injections for drooling in children with cerebral palsy. Data sources: We searched the EMBASE, MEDLINE, Cochrane Database of Systematic Reviews, and Cochrane Central Register of Controlled Trials (CENTRAL, The Cochrane Library) databases from inception to January 2020. Methods: We included randomized controlled trials and observational studies which (1) involved children with cerebral palsy, (2) used BoNT-A for control of drooling, and (3) provided quantitative evaluations of drooling before and after intervention with BoNT-A. Results: Twenty-one trials met the inclusion criteria. Most studies showed that BoNT-A injections are safe and efficacious as a treatment for drooling in children with cerebral palsy. Four trials had sufficient data to pool the results for the meta-analysis. Both the drooling quotient (p = 0.002) and drooling Ffrequency and severity scale (p = 0.004) supported this conclusion. Conclusion: BoNT-A injections are a safe, reversible, effective treatment for drooling control in children with cerebral palsy that can offer effectiveness for more than 3 months with few side effects. The dosage of BoNT-A should not exceed 4 units/kg. Further studies are required to determine the optimal dosage and target glands.
Floral Patches and Their Impact on Pollinator Attraction and Yield Production on Cucurbita maxima Var. Paine in Central Chile
Authors: Donoso Santiago , Murúa Maureen
Published: November 2021

In recent decades, it has been shown that pollination deficit can significantly affect crop production. Moreover, now it is known that the appropriate management of pollination can increase the quantity and quality of yields. Therefore, pollination appears to be a key component in modern agriculture. This study aimed to determine the impact of floral patch addition on the quality and quantity of Cucurbita maxima fruit production. To this end, we manually added a set of ornamental plant species to the edge of the crop and monitored the pollinator diversity and visit frequency, as well as the seed set and sugar content of the pumpkin yield, in treatment and control parcels. The results showed that there was a higher visit rate in the treated parcel. Only the sugar content, and not the seed set, was affected by the addition of ornamental species and the pollinator visit rate. These findings indicate that the addition of flowering plants can favor different components of the crop yield of this species, but future studies should sample on a broader spatial and temporal scale in order to certify the generalizability of our results.
Crustaceans and Marine Heterobranchia: A New Symbiotic Relationship in the Mediterranean Sea
Authors: Furfaro Giulia , Solca Michele , Mariottini Paolo
Published: November 2021

The “solar-powered” Elysia timida (Risso, 1818) is an endemic Mediterranean sacoglossan living in rocky substrates at shallow water. During a scuba dive, one E. timida was photographed and collected. The observation revealed the presence of a small crustacean of the Pinnotheridae family. It was not possible to assign the crustacean to a genus, but it shares typical ecological and external morphological features with other Pinnotherinae species. This is the first report of a pea crab hosted by a non-shelled Heterobranchia and the first case of a symbiotic association between crustaceans and marine Heterobranchia reported in the Mediterranean Sea.
Cultural Practices and Mechanical Weed Control for the Management of a Low-Diversity Weed Community in Spinach
Authors: Gazoulis Ioannis , Kanatas Panagiotis , Antonopoulos Nikolaos
Published: November 2021

Low-diversity weed communities are dominated by few species that are highly competitive to crops. The management of such weed communities should rely upon sustainable cultural and non-chemical practices, especially in crops such as spinach (Spinacia oleracea L.), where very few herbicides are available. A two-year field trial (2020 and 2021) was conducted to evaluate different fertilization practices (broadcast and banded), intra-row spacings (15 cm, 11 cm, 7 cm), and mechanical weed control treatments (untreated, one treatment, two treatments) for the management of a low-diversity weed community in spinach. Weed competition severely affected spinach commercial biomass (R2 = 0.845). Compared to broadcast fertilization, banded fertilization reduced weed biomass and improved spinach yield and nitrogen use efficiency. Narrow intra-row spacing (7-cm) reduced weed biomass by 28 and 45% compared to intra-row spacings of 11-cm and 15-cm, respectively. Two mechanical weed control treatments resulted in 49% lower weed biomass compared to a single treatment. Commercial biomass increased with decreasing intra-row spacing (R2 = 0.881) and increasing the number of mechanical treatments (R2 = 0.911). More cultural and non-chemical practices should be evaluated for weed management in spinach, especially at sites infested with low-diversity weed communities.
Updated Checklist of Vascular Plants Endemic to Mongolia
Authors: Baasanmunkh Shukherdorj , Urgamal Magsar , Oyuntsetseg Batlai , Grabovskaya-Borodina Alisa , Oyundelger Khurelpurev , Tsegmed Zagarjav , Gundegmaa Vanjil , Kechaykin Alexey A. , Pyak Andrey I. , Zhao Li Qing , Choi Hyeok Jae
Published: November 2021

The aim of the present study is to update the checklist of vascular plants endemic to Mongolia using previous checklists, publications, herbarium collections, and field observations. The revised checklist includes 102 endemic taxa (95 species, five subspecies, and two nothospecies) from 43 genera and 19 families. The majority of endemic taxa were distributed in western and central Mongolia, and high endemic species richness was identified in four phytogeographical regions, namely Mongolian Altai, Khangai, Dzungarian Gobi, and Gobi Altai, which harbor 49, 27, 20, and 16 endemic taxa, respectively. For each endemic taxon, we compiled information about growth form, conservation status (if already assessed), phytogeographical distribution, and voucher specimens. Data on each taxon’s type specimen were also collected, and the majority of the type specimens were accessioned at the LE (58 taxa), MW (20 taxa), and UBA (7 taxa) herbaria.
Transcriptome Analyses Reveal Circadian-Related Expression Features in the Visual Systems of Two Snakes
Authors: Tang Chen-Yang , Song Meng-Huan , Peng Zhong-Liang , Wu Wei , Peng Changjun , Yang Kong , Li Jia-Tang
Published: November 2021

The visual characteristics of animals with different circadian habits, especially colubrid snakes, exhibit highly variable photoreceptor morphology. While studies have reported on the diversity in retinal cell morphology among snakes with different circadian patterns, few studies have examined the expression of genes related to vision. To explore gene expression patterns in the eyes between diurnal and nocturnal snakes, we carried out RNA sequencing of six tissues (eye, heart, liver, lung, kidney, and muscle) in two colubrids with disparate circadian activities, i.e., diurnal Ahaetulla prasina and nocturnal Lycodon flavozonatum, followed by weighted gene co-expression network analysis (WGCNA). The genes in the two most correlated modules were primarily enriched in different functional pathways, thus suggesting different biological functions. Three opsin genes (RH1, LWS, and SWS) were differentially expressed between the two species. Moreover, in the phototransduction pathway, different genes were highly expressed in the eyes of both species, reflecting specific expression patterns in the eyes of snakes with different circadian activity. We also confirmed the dominance of cone- and rod-related genes in diurnal and nocturnal adaptation, respectively. This work provides an important foundation for genetic research on visual adaptation in snakes and provides further insight into the adaptive evolution of such species.
Landscape Genetics of the Yellow-Bellied Toad (Bombina variegata) in the Northern Weser Hills of Germany
Authors: Kleißen Jasmin , Balkenhol Niko , Pröhl Heike
Published: November 2021

Anthropogenic influences such as deforestation, increased infrastructure, and general urbanization has led to a continuous loss in biodiversity. Amphibians are especially affected by these landscape changes. This study focuses on the population genetics of the endangered yellow-bellied toad (Bombina variegata) in the northern Weser Hills of Germany. Additionally, a landscape genetic analysis was conducted to evaluate the impact of eight different landscape elements on the genetic connectivity of the subpopulations in this area. Multiple individuals from 15 study sites were genotyped using 10 highly polymorphic species-specific microsatellites. Four genetic clusters were detected, with only two of them having considerable genetic exchange. The average genetic differentiation between populations was moderate (global FST = 0.1). The analyzed landscape elements showed significant correlations with the migration rates and genetic distances between populations. Overall, anthropogenic structures had the greatest negative impact on gene flow, whereas wetlands, grasslands, and forests imposed minimal barriers in the landscape. The most remarkable finding was the positive impact of the underpasses of the motorway A2. This element seems to be the reason why some study sites on either site of the A2 showed little genetic distance even though their habitat has been separated by a strong dispersal barrier.
Focal Cortical Dysplasia Type Ⅲ Related Medically Refractory Epilepsy: MRI Findings and Potential Predictors of Surgery Outcome
Authors: Wang Xiaozhuan , Deng Dabiao , Zhou Chengqian , Li Honglin , Guan Xueqin , Fang Liguang , Cai Qinxin , Wang Wensheng , Zhou Quan
Published: November 2021

This study aims to explore the relationship between neuropathologic and the post-surgical prognosis of focal cortical dysplasia (FCD) typed-Ⅲ-related medically refractory epilepsy. A total of 266 patients with FCD typed-Ⅲ-related medically refractory epilepsy were retrospectively studied. Presurgical clinical data, type of surgery, and postsurgical seizure outcome were analyzed. The minimum post-surgical follow-up was 1 year. A total of 266 patients of FCD type Ⅲ were included in this study and the median follow-up time was 30 months (range, 12~48 months). Age at onset ranged from 1.0 years to 58.0 years, with a median age of 12.5 years. The number of patients under 12 years old was 133 (50%) in patients with FCD type Ⅲ. A history of febrile seizures was present in 42 (15.8%) cases. In the entire postoperative period, 179 (67.3%) patients were seizure-free. Factors with p < 0.15 in univariate analysis, such as age of onset of epilepsy (p = 0.145), duration of epilepsy (p = 0.004), febrile seizures (p = 0.150), being MRI-negative (p = 0.056), seizure type (p = 0.145) and incomplete resection, were included in multivariate analysis. Multivariate analyses revealed that MRI-negative findings of FCD (OR 0.34, 95% CI 0.45–0.81, p = 0.015) and incomplete resection (OR 0.12, 95% CI 0.05–0.29, p < 0.001) are independent predictors of unfavorable seizure outcomes. MRI-negative finding of FCD lesions and incomplete resection were the most important predictive factors for poor seizure outcome in patients with FCD type Ⅲ.
A Hybrid Multi-Criteria Decision-Making Approach Based on ANP-Entropy TOPSIS for Building Materials Supplier Selection
Authors: Chen Chun-Ho
Published: November 2021

This article will tell you how to combine “entropy” in the model to reduce the bias of multi-criteria evaluation. Subjective weights are usually determined by decision makers based on their professional background, experience and knowledge, and other factors. The objective weight is obtained by constructing an evaluation matrix of the information based on the actual information of the evaluation criteria of the scheme, and obtained through multi-step calculations. Different decision-making methods are based on different weight types. Considering only one of the two weights often leads to biased results. In addition, in order to establish an effective supply chain, buyers must find suitable merchants among suppliers that provide quality products and/or services. Based on the above factors, it is difficult to choose a suitable alternative. The main contribution of this paper is to combine analytic network process (ANP), entropy weight and the technique for order preference by similarity to an ideal solution (TOPSIS) to construct a suitable multi-criteria decision (MCDM) model. By means of ANP-entropy weights to extend the TOPSIS method, ANP-entropy weights are used to replace subjective weights. A supplier selection decision-making model based on ANP-entropy TOPSIS is proposed. At last, the sensitivity analysis shows that, taking the selection of building materials suppliers as an example, the hybrid ANP-entropy TOPSIS method can effectively select suitable suppliers.
Simultaneous Audio Encryption and Compression Using Parallel Compressive Sensing and Modified Toeplitz Measurement Matrix
Authors: Cai Changchun , Bai Enjian , Jiang Xue-Qin , Wu Yun
Published: November 2021

With the explosive growth of voice information interaction, there is an urgent need for safe and effective compression transmission methods. In this paper, compressive sensing is used to realize the compression and encryption of speech signals. Firstly, the scheme of linear feedback shift register combined with inner product to generate measurement matrix is proposed. Secondly, we adopt a new parallel compressive sensing technique to tremendously improve the processing efficiency. Further, the two parties in the communication adopt public key cryptosystem to safely share the key and select a different measurement matrix for each frame of the voice signal to ensure the security. This scheme greatly reduces the difficulty of generating measurement matrix in hardware and improves the processing efficiency. Compared with the existing scheme by Moreno-Alvarado et al., our scheme has reduced the execution time by approximately 8%, and the mean square error (MSE) has also been reduced by approximately 5%.
BrainShield: A Hybrid Machine Learning-Based Malware Detection Model for Android Devices
Authors: Rodrigo Corentin , Pierre Samuel , Beaubrun Ronald , El Khoury Franjieh
Published: November 2021

Android has become the leading operating system for mobile devices, and the most targeted one by malware. Therefore, many analysis methods have been proposed for detecting Android malware. However, few of them use proper datasets for evaluation. In this paper, we propose BrainShield, a hybrid malware detection model trained on the Omnidroid dataset to reduce attacks on Android devices. The latter is the most diversified dataset in terms of the number of different features, and contains the largest number of samples, 22,000 samples, for model evaluation in the Android malware detection field. BrainShield’s implementation is based on a client/server architecture and consists of three fully connected neural networks: (1) the first is used for static analysis and reaches an accuracy of 92.9% trained on 840 static features; (2) the second is a dynamic neural network that reaches an accuracy of 81.1% trained on 3722 dynamic features; and (3) the third neural network proposed is hybrid, reaching an accuracy of 91.1% trained on 7081 static and dynamic features. Simulation results show that BrainShield is able to improve the accuracy and the precision of well-known malware detection methods.
Comparative Analysis of Two Speed-Estimation Methods for Dual Three-Phase Induction Motor with Stator Resistance Online Identification
Authors: Xiu Chunwen , Yao Fei , Zheng Jianli
Published: November 2021

The dual three-phase induction motor (DTPIM) has gained wide attention in special applications, such as vessel propulsion, because of its advantages of less torque ripple and higher reliability. However, speed sensors are greatly affected and easily become faulty when used in harsh environments for a long time. In this paper, two model reference adaptive system (MRAS) speed-estimation methods are proposed, based on the double (α, β) coordinate system (DCS) and vector space decomposition method (VSD) of the two groups of the three-phase armature vectors, respectively. Both methods can be used for the speed sensorless control system of the DTPIM to improve reliability. The changing of the stator resistance value, caused by temperature variation, affects the accuracy of the speed-estimation. Two online resistance-identification algorithms, combining the DCS method and the VSD method, were proposed to reduce the effect of changes in stator resistance. Simulation results show that the dynamic speed-estimation error of the VSD method decreased greatly compared with the DCS method, which verifies the effectiveness of the theoretical analysis.
Finding More Property Violations in Model Checking via the Restart Policy
Authors: Geng Mengtao , Zhang Xiaoyu , Li Jianwen
Published: November 2021

Model checking is an efficient formal verification technique that has been applied to a wide spectrum of applications in software engineering. Popular model checking algorithms include Bounded Model Checking (BMC) and Incremental Construction of Inductive Clauses for Indubitable Correctness/Property Directed Reachability(IC3/PDR). The recently proposed Complementary Approximate Reachability (CAR) model checking algorithm has a performance close to BMC in bug-finding, while its depth-first strategy sometimes leads the algorithm to a trap, which will waste lots of computation. In this paper, we enhance the recently proposed Complementary Approximate Reachability (CAR) model checking algorithm by integrating the restart policy, which yields a restartable CAR model (abbreviated as r-CAR). The restart policy can help avoid the trap problem caused by the depth-first strategy and has played an important role in modern SAT-solving algorithms to search for a satisfactory solution. As the bug-finding in model checking is reducible to a similar search problem, the restart policy can be useful to enhance the bug-finding capability. We made an extensive experiment to evaluate the new algorithm. Our results show that out of the 749 industrial instances, r-CAR is able to find 13 instances that the state-of-the-art BMC technique cannot find and can solve more than 11 instances than the original CAR. The new algorithm successfully contributes to the current model-checking portfolio in practice.
Free Switching Control Strategy for Multi-Operation Modes of Multi-Port Energy Router in Distribution Area
Authors: Chi Shumei , Lv Zhipeng , Liu Lan , Shan Yang
Published: November 2021

For the distribution area with a high penetration rate of new energy, the traditional power supply system has some problems, such as a single form of power supply and low utilization of new energy. Because the multi-port energy router can realize the interconnection and complementation of multiple energy forms, it has become the key piece of equipment in the hybrid AC/DC distribution area. Nevertheless, restricted by the existing control strategy, the performance of the energy router in complex operation mode switching and coordinated control still needs to be further improved. To address this issue, the free switching control strategy is proposed in this paper. Firstly, the topology and model of the multi-port energy router are designed and established. Secondly, the operation mode of the system is analyzed, and the control strategy of each port is designed. Then, a reference power calculation method suitable for multi-mode operation is derived. Based on this, the control strategy does not need to be changed when operation modes are switched. Furthermore, the extended state observer is introduced to track and compensate for the new energy disturbance, which can improve the power quality of the system. Finally, the simulation and experimental results show that the proposed control strategy of the multi-port energy router can realize flexible and controllable power transmission among various modules in the distribution area and the free switching of multi-operation modes without changing the control strategy.
Synthesis and Comprehensive Study of Quaternary-Ammonium-Based Sorbents for Natural Gas Sweetening
Authors: Atlaskina Maria E. , Atlaskin Artem A. , Kazarina Olga V. , Petukhov Anton N. , Zarubin Dmitriy M. , Nyuchev Alexander V. , Vorotyntsev Andrey V. , Vorotyntsev Ilya V.
Published: November 2021

The present study provides a solvent-free organic synthesis of quaternary ammonium salts: bis(2-hydroxyethyl)dimethylammonium taurate ([BHEDMA][Tau]) and bis(2-hydroxyethyl)dimethylammonium acetate ([BHEDMA][OAc]). These ionic compounds are promising materials for carbon dioxide capture processes, as mono sorbents, supplemental components in the conventional process of chemical absorption, and in the combined membrane approach for improving sorption efficiency. The synthesized compounds were characterized by 1H NMR and FT-IR spectroscopies and elemental analysis. Afterward, the sorption properties of the compounds were evaluated using the inverse gas chromatography (IGC) method, and their thermodynamic parameters were calculated in the temperature range of 303.15–333.15 K. The enthalpy change (∆sH) was less than 80 kJ·mol−1, indicated by the physical nature of sorption and also proved by FT-IR. Henry’s law constant in regard to carbon dioxide at 303.15 K was equal to 4.76 MPa for [BHEDMA][Tau], being almost 2.5 lower than for [BHEDMA][OAc] (11.55 MPa). The calculated carbon dioxide sorption capacity for [BHEDMA][Tau] and [BHEDMA][OAc] amounted to 0.58 and 0.30 mmol·g−1, respectively. The obtained parameters are comparable with the known solid sorbents and ionic liquids used for CO2 capture. However, the synthesized compounds, combining the advantages of both alkanolamines and ionic liquids, contain no fluorine in their structure and thus match the principles of environmental care.
PHYTO-THREATS: Addressing Threats to UK Forests and Woodlands from Phytophthora; Identifying Risks of Spread in Trade and Methods for Mitigation
Authors: Green Sarah , Cooke David E. L. , Dunn Mike , Barwell Louise , Purse Bethan , Chapman Daniel S. , Valatin Gregory , Schlenzig Alexandra , Barbrook Jane , Pettitt Tim , Price Colin , Pérez-Sierra Ana , Frederickson-Matika Debra , Pritchard Leighton , Thorpe Peter , Cock Peter J. A. , Randall Eva , Keillor Beatrix , Marzano Mariella
Published: November 2021

The multidisciplinary ‘Phyto-threats’ project was initiated in 2016 to address the increasing risks to UK forest and woodland ecosystems from trade-disseminated Phytophthora. A major component of this project was to examine the risk of Phytophthora spread through nursery and trade practices. Close to 4000 water and root samples were collected from plant nurseries located across the UK over a three-year period. Approximately half of the samples tested positive for Phytophthora DNA using a metabarcoding approach with 63 Phytophthora species identified across nurseries, including quarantine-regulated pathogens and species not previously reported in the UK. Phytophthora diversity within nurseries was linked to high-risk management practices such as use of open rather than closed water sources. Analyses of global Phytophthora risks identified biological traits and trade pathways that explained global spread and host range, and which may be of value for horizon-scanning. Phytophthoras having a higher oospore wall index and faster growth rates had wider host ranges, whereas cold-tolerant species had broader geographic and latitudinal ranges. Annual workshops revealed how stakeholder and sector ‘appetite’ for nursery accreditation increased over three years, although an exploratory cost-benefit analysis indicated that the predicted benefits of introducing best practice expected by nurseries outweigh their costs only when a wider range of pests and diseases (for example, Xylella) is considered. However, scenario analyses demonstrated the significant potential carbon costs to society from the introduction and spread of a new tree-infecting Phytophthora: Thus, the overall net benefit to society from nurseries adopting best practice could be substantial.
The Root Mycobiota of Betula aetnensis Raf., an Endemic Tree Species Colonizing the Lavas of Mt. Etna (Italy)
Authors: Badalamenti Emilio , Catania Valentina , Sofia Serena , Sardina Maria Teresa , Sala Giovanna , La Mantia Tommaso , Quatrini Paola
Published: November 2021

Betula aetnensis is an endemic tree of high conservation value, which thrives on the nutrient-poor volcanic soils of Mount Etna. Since plant–microbe interactions could play a crucial role in plant growth, resource uptake, and resistance to abiotic stresses, we aimed to characterize the root and rhizosphere microbial communities. Individuals from natural habitat (NAT) and forest nursery (NURS) were surveyed through microscopy observations and molecular tools: bacterial and fungal automated ribosomal intergenic spacer analysis (ARISA), fungal denaturing gradient gel electrophoresis (DGGE). B. aetnensis was found to be simultaneously colonized by arbuscular (AM), ectomycorrhizal (ECM), ericoid (ERM) fungi, and dark septate endophytes (DSE). A high diversity of the bacterial community was observed whilst the root fungal assemblage of NAT plants was richer than that of NURS. Root and rhizosphere fungal communities from NAT plants were characterized by Illumina MiSeq sequencing. Most of the identified sequences were affiliated to Helotiales, Pezizales, and Malasseziales. Ascomycota and Basidiomycota dominated roots and rhizosphere but differed in community structure and composition. ECM in the roots mainly belonged to Tylospora and Leccinum, while Rhizopogon was abundant in the rhizosphere. The Helotiales, including ERM (mostly Oidiodendron) and DSE (mostly Phialocephala), appeared the dominant component of the fungal community. B. aetnensis harbors an extraordinarily wide array of root-associated soil microorganisms, which are likely to be involved in the adaptation and resistance mechanisms to the extreme environmental conditions in volcano Etna. We argue that nursery-produced seedlings could lack the necessary microbiota for growth and development in natural conditions.
Transcriptome-Wide Identification and Expression Profiling of SPX Domain-Containing Members in Responses to Phosphorus Deprivation of Pinus massoniana
Authors: Wang Conghui , Fan Fuhua , Shang Xianwen , Zhou Zijing , Ding Guijie
Published: November 2021

The SPX domain-encoding proteins are believed to play important roles in phosphorus (Pi) homeostasis and signal transduction in plants. However, the overall information and responses of SPXs to phosphorus deficiency in pines, remain undefined. In this study, we screened the transcriptome data of Pinus massoniana in response to phosphorus deprivation. Ten SPX domain-containing genes were identified. Based on the conserved domains, the P. massoniana SPX genes were divided into four different subfamilies: SPX, SPX-MFS, SPX-EXS, and SPX-RING. RNA-seq analysis revealed that PmSPX genes were differentially expressed in response to phosphorus deprivation. Furthermore, real-time quantitative PCR (RT-qPCR) showed that PmSPX1 and PmSPX4 showed different expression patterns in different tissues under phosphorus stress. The promoter sequence of 2284 bp upstream of PmSPX1 was obtained by the genome walking method. A cis-element analysis indicated that there were several phosphorus stress response-related elements (e.g., two P1BS elements, a PHO element, and a W-box) in the promoter of PmSPX1. In addition, the previously obtained PmSPX2 promoter sequence contained a W-box, and it was shown that PmWRKY75 could directly bind to the PmSPX2 promoter using yeast one-hybrid analysis in this study. These results presented here revealed the foundational functions of PmSPXs in maintaining plant phosphorus homeostasis.
Quercus Genetics: Insights into the Past, Present, and Future of Oaks
Authors: Backs Janet R. , Ashley Mary V.
Published: November 2021

The genus Quercus comprises over 400 species found across the Northern Hemisphere [...]
Effect of Polyethylene Glycol Treatment on Acetic Acid Emissions from Wood
Authors: Hunt Sarah , Grau-Bove Josep , Schofield Eleanor , Gaisford Simon
Published: November 2021

Acetic acid is known to be emitted from sound wood and can accelerate damage to heritage materials, particularly metals. However, few studies have investigated the extent of acetic acid emissions from archaeological wood. This research utilised Solid-Phase-Micro-Extraction (SPME) GC–MS and lead coupon corrosion to identify volatile emissions from polyethylene glycol (PEG)-treated archaeological wood from the Mary Rose collection and assess if they could cause accelerated damage. In addition, the effect of PEG treatment on acetic acid emissions was investigated using sound wood samples. For sound wood, the PEG treatment acted as a barrier to acetic acid emissions, with higher-molecular-weight PEGs preventing more emissions. Archaeological wood, despite its age and high-molecular-weight PEG treatment, still emitted detectable concentrations of acetic acid. Moreover, they emitted a wider array of compounds compared to sound wood, including carbon disulphide. Like sound wood, when the archaeological wood samples were in a sealed environment with lead coupons, they caused accelerated corrosion to lead. This evidences that archaeological wood can emit high enough concentrations of volatile compounds to cause damage and further investigation should be performed to evaluate if this can occur inside museum display cases.
Influence of Mechanical Wounding and Compartmentalization Mechanism on the Suppression of Invasive Plant Species Using the Example of Cherry Laurel (Prunus laurocerasus)
Authors: Plavčak Denis , Mikac Urša , Merela Maks
Published: November 2021

Natural habitats increasingly face the introduction and spread of non-native species. Under the right conditions, non-native species can become invasive over time. This issue is now being addressed by many experts and researchers who are using and developing various approaches and methods to limit and eliminate or suppress problematic plant species. Many invasive plants are already spreading uncontrollably in urban and forestry areas, causing health hazards, environmental and economic damage and negatively impacting natural ecosystems. The use of chemical agents is generally limited, so our only option to control and suppress the problematic species is mechanical removal. In this research suppression by tree stem wounding, i.e., incomplete girdling, was used. This type of injury causes the plant to lose its vitality, become weaker after first year and then die within a few years. Using a research approach, we chronologically monitored the response of cherry laurel (Prunus laurocerasus L.) stem tissue to mechanical wounding of the incomplete girdling. Magnetic resonance imaging (MRI) and light microscopy were used for monitoring moisture content and anatomical changes in different periods after injury. The results of the study showed that cherry laurel, with an intense wound tissue response and other changes, is a species with good compartmentalization potential. The rapid and intense tissue response to injury requires high energy and nutrient consumption and consequently leads to a loss of vigour and mechanical stability, which may result in plant destruction. Results revealed that mechanical wounding by incomplete girdling is a successful method for suppression of non-native and invasive cherry laurel.
Endophytic Community Composition and Genetic-Enzymatic Features of Cultivable Bacteria in Vaccinium myrtillus L. in Forests of the Baltic-Nordic Region
Authors: Mažeikienė Ingrida , Frercks Birutė , Burokienė Daiva , Mačionienė Irena , Šalaševičienė Alvija
Published: November 2021

Regardless of their growth locations and species diversity, plants have endophytic bacterial communities. Bilberry (Vaccinium myrtillus L.) is valuable for human health because of its antioxidant properties, and the plant has adapted to stressful growing conditions in forests. Here, we aimed to describe the composition of the community of endophytic microorganisms in bilberry leaves and to determine whether the diversity of endophytic bacteria varies depending on the geographical location of the plants. In this study, we evaluated the significance of endophytes in the host plant and the potential use of such bacteria. Twenty-five culturable bacterial isolates were identified in V. myrtillus leaves based on 16S rRNA gene sequencing and phylogenetic analysis. For the first time, we report upon the diversity of endophytic communities coexisting in bilberry leaves in different geographical locations of the Nordic-Baltic region. Under harsh conditions, the bilberry plants had a greater diversity of bacteria. The bacterial families Bacillaceae, Paenibacillaceae and Micrococcaceae were the most common endophytes in leaves of bilberry. Strains of Staphylococcaceae, Lactobacillaceae, Pseudomonaceae, Corynebacteriaceae and Planococcaceae were identified in samples from Finland and Norway. Plant growth-promoting genes (acdS and AcPho) and/or enzymatic activity were identified in many isolates.
Soil Water Stable Isotopes Reveal Surface Soil Evaporation Loss Dynamics in a Subtropical Forest Plantation
Authors: Lyu Sidan , Wang Jing
Published: November 2021

Line-conditioned excess (lc-excess), the deviation of the relationship between δD and δ18O in soil water from that of precipitation, is often used to indicate soil evaporation loss, but the conditions of using lc-excess under the influences of precipitation infiltration or percolation had not been identified. The interaction effects of climate, soil and vegetation on soil evaporation in forests are not well known. We collected soil water at 0–5, 15–20 and 40–45 cm depths and event-based precipitation from 2011 to 2015 in a subtropical forest plantation and calculated the lc-excess. Precipitation on the sampling day and percolation of upper soil water with low lc-excess affected the capacity of the lc-excess to indicate the soil evaporation fractionation signals. Lc-excess of soil water at 0–5 cm depth indicated a reliable soil evaporation loss estimate over 30 days prior to the sampling day. Soil evaporation loss was dominated by the cumulative soil temperature (Tss) during drought periods and was dominated by the relative soil water content (RSWC) during non-drought periods. High Tss decreased soil evaporation loss by increasing transpiration and relative humidity. Our results emphasize the importance of sampling the upper-most soil layer when there is no rain and vegetation during drought periods in forests when studying soil evaporation loss dynamics.
The Selective Effects of Environmental Change on the Functional Diversity of Soil Decomposers
Authors: Verhoef Herman A.
Published: November 2021

Whether decomposition can be affected by the biodiversity of soil organisms is an important question. Biodiversity is commonly expressed through indices that are based on species richness and abundances. Soil processes tend to saturate at low levels of species richness. A component of biodiversity is functional diversity, and we have shown that the absence of the influence of species richness on decomposition switched into a positive relationship between fauna diversity and decomposition when we expressed biodiversity in terms of interspecific functional dissimilarity. Communities with functionally dissimilar species are characterized by complementary resource use and facilitative interactions among species. It is suggested that the effects of environmental changes on ecosystem functions such as decomposition can be better understood if we have more knowledge about the selective effect of these changes on specific facets of soil biodiversity, such as functional diversity.