bluuebunny/crossref_metadata_2025 huggingface dataset:
Alexithymia as a mediator between adverse childhood events and the development of psychopathology: a meta-analysis
Authors: Kick Lorenz , Schleicher Daniel , Ecker Angelika , Kandsperger Stephanie , Brunner Romuald , Jarvers Irina
Published: July 2024

Introduction

Victims of child abuse have an elevated risk of developing mental health issues later in life. Several variables have been suggested as mediators of this correlation, but little is known about the possible influence of alexithymia. Alexithymia is a sub-clinical personality trait that manifests as difficulties recognizing and verbalizing emotions.

Methods

In this study, two separate meta-analyses were conducted using questionnaire data, and Pearson correlations for overall effects were estimated.

Results

The correlation between child abuse and alexithymia showed to be significant (r= .26), as did the correlation between alexithymia and general psychopathology (r= .44). Further analyses revealed no indication for possible publication bias. When investigating differences between various subtypes of child maltreatment, each subtype significantly correlated with alexithymia. Emotional abuse, emotional neglect, and physical neglect had stronger correlations than physical and sexual abuse.

Discussion

These results suggest that alexithymia plays a mediating role, at least in part, in the relationship between experiences of child abuse and general psychopathology in adulthood. Therefore, alexithymia may be relevant to further research and deserves attention in the prevention of and therapy for mental health issues in victims of child abuse.
Stormwater quality and microbial ecology in an urban rain garden system
Authors: Corbett Erin , Ijaz Umer Zeeshan , Jackson Isla , Phoenix Vernon R.
Published: July 2024

Rain gardens are an alternative to traditional drainage, able to lower flood risk and reduce environmental contamination from stormwater. Removal of contaminants by rain gardens is driven by both physical processes (such as filtration and sedimentation) and biological metabolic processes by soil microorganisms. To better understand rain garden performance, this study explored the impact of rain gardens on pollution removal and microbial composition and function using rain gardens fed real stormwater from a busy road. Each rain garden had different grain size and hydraulic conductivities as these parameters have been argued to impact pollution removal. All four rain gardens were able to reduce the contaminant load in the stormwaters, reducing the concentration of dissolved metals, suspended solids and chemical oxygen demand. Significantly, road salting in the winter did not cause dissolved metals to be released from the rain gardens, suggesting that rain gardens can continue to reduce contaminant loads during winter salting regimes. Some variation in pollutant removal was seen between the soils tested, but overall no clear trend could be identified based on grain size and hydraulic conductivity with all rain gardens performing broadly similarly. The rain garden soil altered the microbial community in the stormwater, resulting in greater taxonomic evenness and functional richness in the effluent water compared to the influent. Functional richness of the soils was also higher than that of the input waters, indicating that the microbes in the rain gardens were able to perform a wider range of functions than those of the influent. Effluent and soil microbiology was more impacted by sampling date than soil grain size, which may be a result of the soil communities maturing and changing over time. As greater numbers of rain gardens are installed to tackle flooding from climate change, it is important to ensure the environment is protected from urban contaminants in the stormwater. The results in this study further highlight the ability of rain gardens to undertake this important task.
Solar-Powered Life: How Plants And Other Organisms Produce Their Own Food
Authors: Aragón Lina , Feeley Kenneth J.
Published: July 2024

Some organisms can produce their own food through a process called photosynthesis. These organisms transform light energy, carbon dioxide, and water into sugars, which allow them to grow their bodies, reproduce, and be a source of energy for other organisms. Studying photosynthesis in nature and in the laboratory has given scientists important insights into the effects of climate change on plants and other photosynthetic organisms. For example, such studies help scientists understand whether there will continue to be enough food for humans to eat as the climate changes. In this article, we discuss the importance of photosynthetic organisms; how light energy, carbon dioxide, and water are transformed into sugar during photosynthesis; the challenges that today’s land plants face; and how and why scientists measure photosynthesis in plants.
Effect of transbronchial or intravenous administration of indocyanine green on resection margins during near-infrared-guided segmentectomy: a review
Authors: Libor László , Pécsy Balázs , Szűcs Evelin , Lantos Judit , Bakos Annamária , Lázár György , Furák József
Published: July 2024

For early-stage non-small cell lung cancer, surgical resection remains the best treatment option. Currently, sublobar resection, including segmentectomy, is recommended in these cases, as it provides a better quality of life with the same oncological outcomes; however, is requires adequate resection margins. Accurate preoperative planning and proper identification of the intersegmental planes during thoracic surgery are crucial for ensuring precise surgical management and adequate resection margins. Three dimensional computed tomography reconstruction and near-infrared-guided intersegmental plane identification can greatly facilitate the surgical procedures. Three-dimensional computed tomography reconstruction can simulate both the resection and resection margins. Indocyanine green is one of the most frequently used and affordable fluorophores. There are two ways to identify the intersegmental planes using indocyanine green: intravenous and transbronchial administration. Intravenous application is simple; however, its effectiveness may be affected by underlying lung disease, and it requires the isolation of segmental structures before administration. Transbronchial use requires appropriate bronchoscopic skills and preoperative planning; however, it also allows for delineation deep in the parenchyma and can be used for complex segmentectomies. Both methods can be used to ensure adequate resection margins and, therefore, achieve the correct oncological radicality of the surgical procedure. Here, we summarise these applications and provide an overview of their different possibilities.
Recombinase polymerase amplification combined with lateral flow biosensor for rapid visual detection of Clostridium perfringens in chicken meat and milk
Authors: Tian Rui , Xie Feng , Liu Yuqing , Liu Guangjin , Li Qingxia , Wang Jinxiu , Zhang Hongjian , Dai Lei , Zhang Wei
Published: July 2024

Aims

Clostridium perfringens is one of the major anaerobic pathogen causing food poisoning and animal enteritis. With the rise of antibiotic resistance and the restrictions of the use of antibiotic growth promoting agents (AGPs) in farming, Clostridium enteritis and food contamination have become more common. It is time-consuming and labor-intensive to confirm the detection by standard culture methods, and it is necessary to develop on-site rapid detection tools. In this study, a combination of recombinase polymerase amplification (RPA) and lateral flow biosensor (LFB) was used to visually detect C. perfringens in chicken meat and milk.

Methods and results

Two sets of primers were designed for the plc gene of C. perfringens, and the amplification efficiency and specificity of the primers. Selection of primers produces an amplified fragment on which the probe is designed. The probe was combined with the lateral flow biosensor (LFB). The reaction time and temperature of RPA-LFB assay were optimized, and the sensitivity of the assay was assessed. Several common foodborne pathogens were selected to test the specificity of the established method. Chicken and milk samples were artificially inoculated with different concentrations (1 × 102 CFU/mL to 1 × 106 CFU/mL) of C. perfringens, and the detection efficiency of RPA-LFB method and PCR method was compared. RPA-LFB can be completed in 20 min and the results can be read visually by the LFB test strips. The RPA-LFB has acceptable specificity and the lowest detection limit of 100 pg./μL for nucleic acid samples. It was able to stably detect C. perfringens contamination in chicken and milk at the lowest concentration of 1 × 104 CFU/mL and 1 × 103 CFU/mL, respectively.

Conclusion

In conclusion, RPA-LFB is specific and sensitive. It is a rapid, simple and easy-to-visualize method for the detection of C. perfringens in food and is suitable for use in field testing work.
The Novel EfficientNet Architecture-Based System and Algorithm to Predict Complex Human Emotions
Authors: Khomidov Mavlonbek , Lee Jong-Ha
Published: July 2024

Facial expressions are often considered the primary indicators of emotions. However, it is challenging to detect genuine emotions because they can be controlled. Many studies on emotion recognition have been conducted actively in recent years. In this study, we designed a convolutional neural network (CNN) model and proposed an algorithm that combines the analysis of bio-signals with facial expression templates to effectively predict emotional states. We utilized the EfficientNet-B0 architecture for network design and validation, known for achieving maximum performance with minimal parameters. The accuracy for emotion recognition using facial expression images alone was 74%, while the accuracy for emotion recognition combining biological signals reached 88.2%. These results demonstrate that integrating these two types of data leads to significantly improved accuracy. By combining the image and bio-signals captured in facial expressions, our model offers a more comprehensive and accurate understanding of emotional states.
Review of Sustainability Accounting Terms
Authors: Jankalová Miriam , Jankal Radoslav
Published: July 2024

Sustainability accounting is considered a subcategory of financial accounting that focuses on the disclosure of non-financial information about a company’s performance to external parties. Various definitions of sustainability accounting by different authors raise two research questions: RQ1: What is understood by sustainability accounting, as well as which terms are used in relation to sustainability accounting by different authors and organisations? RQ2: What is the relationship between sustainability accounting and the different names used for this term? The aim of this paper is to describe sustainability accounting, identify the terms that are used in relation to sustainability accounting by different authors and organisations, and identify the relationship between sustainability accounting and the different names used for this term. This study is based on information that was gathered through an extensive literature review (research publications and research studies (documents), using the Internet and research databases, and the author’s own experience. Methods of analysis, comparison, selection, abstraction, induction, deduction, determination, and statistics were used. This study presents a comprehensive bibliometric analysis in the field of accounting terms. As a result of this study, the term sustainability accounting is defined, as well as its different interpretations and their synonyms, including identification of the relationship between sustainability accounting and the different names used for this term. Sustainability accounting is an essential part of the future of accounting; it includes the TBL quantification of the company’s activities, products, and services and integrates sustainability metrics into financial reporting.
Performance Evaluation of Three Peanut Cultivars Grown under Elevated CO2 Concentrations
Authors: Novello Nicola , Johnson Joel B. , Laza Haydee , Walsh Kerry B. , Naiker Mani
Published: July 2024

This study explored the performance and physiological responses of three commercially used peanut cultivars in Australian farming systems under ambient and elevated CO2 conditions, aiming to identify the most suitable genotype for dual-purpose (grain and graze) cropping experiments. The experiment utilized an open-top chamber (OTC) facility to regulate CO2 concentrations. The elevated CO2 (EC) treatment targeted approximately 650 ± 50 µmol mol−1, while both ambient CO2 (AC) and control plots operated at a concentration of approximately 400 µmol mol−1. Notably, control plots without chambers served as a reference for current CO2 and environmental conditions. In contrast, despite having the same ambient CO2 concentration, AC plots were enclosed in chambers, allowing for plant growth comparisons with EC plots with the same environmental conditions aside from CO2 levels. The analyses revealed significant effects of CO2 enrichment on peanut plants. In particular, the EC treatment led to enhanced photosynthetic rates (20% in Kairi, 31% in Holt, and 19% in Alloway), alongside reduced stomatal conductance (−55% in Kairi, −32% in Holt, and −40% in Alloway), transpiration, and increased water use efficiency compared to AC conditions. Elevated CO2 levels positively influenced pod yields in Kairi (+41%) and Alloway (+36%). However, CO2 enrichment did not significantly alter the protein content, total phenolic content, cupric-reducing antioxidant capacity, and ferric-reducing antioxidant power of peanut plant material. Furthermore, no significant differences were observed in the phytochemical composition among the three cultivars under ambient or elevated CO2 conditions. On the other hand, analysis of the fibre structure conducted on peanut stover harvested at plant maturity suggested potential declines in feedstock quality. Based on the findings of this research, further investigations and testing, including simulated grazing trials, will be carried out to identify a single breed line suitable for dual-purpose management under future elevated CO2 conditions.
Unravelling the Biochemical and Molecular Priming Effect of a New Yeast-Derived Product: New Perspectives towards Disease Management
Authors: Scimone Giulia , Vicente Isabel , Bartalena Guido , Pisuttu Claudia , Mariotti Lorenzo , Risoli Samuele , Pellegrini Elisa , Sarrocco Sabrina , Nali Cristina
Published: July 2024

Plants constantly face the environment that surrounds them and fight for survival against biotic and abiotic stress factors. To deal with harmful conditions, plants have developed a multilayer defence system, making them capable of recognising threats and promptly recovering from them. This phenomenon, which takes advantage of the “memory effect”, is referred to as bio-priming and represents a new frontier in terms of crop protection. Here, we investigated the “indirect” protective mechanisms of a new yeast extract formulate in Vitis vinifera cv. Sangiovese plants at both the biochemical and genic levels. The formulate was applied once a week for three consecutive weeks, and grapevine leaves were sampled from the first to the fifth day after treatment (dat) at every week of the experiment. Increased levels of jasmonic acid (every week at 2 dat; +70% as average) and abscisic acid (at 1 dat of the first week, more than 1.7-fold higher than the control) and the underproduction of salicylic acid (from 2 dat; −18%) confirmed that these signalling molecules/”specialised compounds” are actively involved in the early activation of defence pathways in treated vines. In addition, pr2 and chit1b, two genes involved in regulating hormonal crosstalk, were significantly up-regulated (both in the first and second week of the trial) and were also found to underlie upstream molecular activation. The results obtained by this investigation confirm the use of this new product to prime and protect grapevines from a wide range of fungal and fungal-like plant pathogens through the induction of defence responses.
Role of Policy-Supported Hog Insurance in Promoting Green Total Factor Productivity: The Case of China during 2005–2021
Authors: Wu Dongli , He Shan , Qin Lingui , Feng Jingyue , Gao Yu
Published: July 2024

Hog insurance and rural environmental protection are complementary to each other. Studying the environmental effects of hog insurance is imperative for safeguarding food safety and promoting the long-term development of the agricultural insurance industry. Informed by the risk management theory and sustainable development theory, this paper constructs a theoretical framework for the impact of policy-supported hog insurance on the green total factor productivity (GTFP) of hog farming. Utilizing panel data from China’s hog-dominant production areas spanning from 2005 to 2021, the slacks-based measures of directional distance functions (SBM-DDF) model and multiple-time-point difference-in-differences (DID) approach were used to measure GTFP and explore the effects of hog insurance on GTFP and the underlying mechanisms. The findings indicate a substantial enhancement in GTFP due to hog insurance. The conclusion drawn was robust to various tests. The mechanism is that hog insurance fosters GTFP by expanding the breeding scale, adjusting the planting–breeding structure, and promoting technological progress. Furthermore, the environmental effects of hog insurance policy are more pronounced in economically developed regions, with significant effects observed on the GTFP of free-range, small-scale, and medium-scale hog-farming households. This study contributes new evidence to the field of assessing the environmental impact of agricultural insurance policies and provides valuable insights for furthering green transformation and development in the hog insurance-supported breeding industry.
Effect of Raised Flat Bed and Ridge Planting on Wheat Crop Growth and Yield under Varying Soil Moisture Depletions
Authors: Tang Lingdi , Shaikh Irfan Ahmed , Tunio Anees , Junejo Abdul Rahim , Hao Li , Dahri Jahangeer , Mangrio Munir Ahmed , Soothar Rajesh Kumar , Khan Zaheer Ahmed
Published: July 2024

Crop yields remain significantly low in underdeveloped countries, such as Pakistan, primarily due to the prevalent use of traditional farming methods by most farmers. Resource-saving strategies, like ridge and raised flat bed systems, could boost water use efficiency and production per acre. However, further research is needed to investigate the effectiveness of these methods, along with different levels of soil moisture depletion (SMD), on wheat development in the climatic conditions of Sindh Province. Thus, field trials were undertaken at Sindh Agriculture University’s Tandojam for two years (2022 and 2023) during the wheat growing seasons. The experiment included six treatments, viz., T1 (raised flat bed method under 40% depletion), T2 (ridge method under 40% depletion), T3 (raised flat bed method under 50% depletion), T4 (ridge method under 50% depletion), T5 (raised flat bed method under 60% depletion), and T6 (ridge method under 60% depletion). The bed planter was employed to make raised flat beds, and the ridges were prepared using a ridge planter. Wheat variety (TJ-83) was sown. Under all treatments, the plant population, plant height, and seed index were statistically significant (p < 0.05), although spike length, grain spikes−1, and grain weight spikes−1 were non-significant at different SMD levels. To compute water saving through the ridge and raised flat methods, the recommended delta value for wheat crops under the traditional method was used as a benchmark. Both irrigation technologies conserve water compared to traditional irrigation methods. The T5 treatment conserved the most water, followed by T6. Under a 60% depletion level, the ridge approach produced the highest yield of 2175 kg ha−1, compared to 601 kg ha−1 with the raised flat bed method. The best crop water productivity (CWP) of 1.34 kg m−3 was achieved under T6, whereas raised flat beds attained the lowest CWP of 0.27 kg m−3 under T1. In terms of wheat grain production and CWP, the ridge furrow method outperformed the raised flat bed furrow method. Based on the results, it is recommended that farmers should use the ridge furrow method and allow a 60% depletion of soil moisture to obtain a high yield of wheat crops.
Life Cycle Assessment and Life Cycle Costing for the Production of Hydrangeas in Antioquia—Colombia
Authors: Arenas Cindy Natalia , Bello Ana Patricia , Molina Nicolas Fernando , Botero Jaime Leòn , Betancur Mariluz
Published: July 2024

In the floriculture sector, it has been identified that the use of agrochemicals, fuels, and various raw materials has a significant impact on the environment, and, from an economic point of view, their use improves the quality of life of the people involved in the activity, and at the same time contributes to the development of the territories. Therefore, in order to address these issues, sustainability-oriented solutions have been proposed. This study focuses on the performance of a Life Cycle Assessment (LCA) of hydrangea production, addressing its environmental and economic dimensions, using the conventional method according to ISO 14040 and ISO 14044 standards. The functional unit was defined as one kilogram (1 kg) of hydrangea stems from a 36-week production cycle in three different crop sizes, as follows: small (0.45 ha), medium (1.20 ha), and large (2.99 ha). The boundaries of the gate-to-gate system were used, including the stages of growing and transporting of the flowers, to delivery to the marketer. The results showed significant environmental impacts in the areas of climate change, human toxicity, and acidification, resulting from phytosanitary management; use of fertilizers, fuel, and raw materials for infrastructure; and packaging of flowers for delivery to the marketer. In addition, from an economic point of view, it was shown that the most significant internal costs were associated with the cultivation phase, while the external costs were associated with CO2 emissions. The return on investment was less than 0.15 years, with human productivity more than 73%, and infrastructure investment less than 16%. These results highlight the need to implement effective measures to mitigate negative impacts and promote more sustainable practices in floriculture to further strengthen the sector, as hydrangeas are an export product.
Revisiting the Potential of Seed Nutri-Priming to Improve Stress Resilience and Nutritive Value of Cereals in the Context of Current Global Challenges
Authors: Houmani Hayet , Ben Slimene Debez Imen , Turkan Ismail , Mahmoudi Henda , Abdelly Chedly , Koyro Hans-Werner , Debez Ahmed
Published: July 2024

Most crop species are cultivated in nutrient-deficient soils, in combination with other challenging constraints that are exacerbated by the current climate changes. The significance of micronutrient shortage in stress management is often underappreciated, although their deficiency restricts both plant growth and resistance to abiotic stresses and diseases. While the application of nutrients to growing plants is a potential strategy to improve plant resistance to abiotic stresses, seed nutrient status may also play a role in crop stress tolerance as a storage and accumulation site of nutrients. To avoid hidden hunger problems, developing countries need to increase domestic cereal production, enhance their resilience to extreme weather events, and improve their nutritional status and quality. Here, we analyze the accumulated knowledge about the effects of nutri-priming in cereal crop species with a focus on mechanisms of application and stress tolerance, keeping in mind the risk of crop damage mostly caused by global climate change, which is driving an alarming increase in the frequency and intensity of abiotic stresses. We also propose new approaches to food production, which may be promising solutions for global warming, emerging diseases, and geopolitical conflicts recognized as major drivers of food insecurity.
Geographical Environment and Plant Functional Group Shape the Spatial Variation Pattern of Plant Carbon Density in Subalpine-Alpine Grasslands of the Eastern Loess Plateau, China
Authors: Xu Manhou , Wang Jiaying , Wei Kunkun , Li Jie , Yu Xiuli
Published: July 2024

The carbon density of subalpine-alpine grasslands (SGs) is significantly vital to sustaining the carbon cycle in global terrestrial ecosystems. However, on the Loess Plateau of China, it remains unclear how the geographical environment and plant functional groups affect the spatial variation pattern of plant carbon density in these grasslands. Here, nine typical SGs distributed in the eastern Loess Plateau with elevations ranging from 1720 to 3045 m were investigated. The biomass indices from grassland plants of different functional groups were investigated using plot surveys. The Kriging interpolation method was used to explore the spatial variation pattern of plant carbon density along geographical gradients. We found that (1) the total plant carbon density of SGs was 2676.825 g C/m2 on the eastern plateau, with 37.07%, 37.50%, and 25.43% contributed by the northern, central, and southern areas, respectively. Above- (666.338 g C/m2) and belowground (2010.488 g C/m2) carbon density accounted for 24.9% and 75.11% of the total, respectively. (2) At the horizontal scale, the plant carbon density in the northern SGs was high in the northwest and low in the southeast; in the central SGs, it was low in the northwest and high in the southeast; and in the southern SGs, it was high in the southwest and low in the northeast. At the vertical scale, plant carbon density in all SGs decreased with increasing altitude. (3) The carbon density of grasses, forbs, and sedges was 247.419 g C/m2, 26.073 g C/m2, and 23.471 g C/m2, respectively. With increased latitude, the carbon density of all functional groups (grasses, forbs, and sedges) decreased; the carbon density of forbs and grasses increased with increased longitude, while that of sedges decreased; and with increased altitude, the carbon density of all functional groups increased. In conclusion, the spatial variation pattern of plant carbon density in the SGs was not only influenced by the geographical environment but also by the plant functional groups at the horizontal and vertical scales on the eastern Loess Plateau of China.
Combining Organic and Inorganic Fertilization Enhances Soil Enzyme Activity, the Bacterial Community, and Molecular Ecological Network Complexity in Coal Mine Reclamation Areas
Authors: Bo Huijuan , Li Zejin , Wang Wei , Zhang Runzhe , Wang Haibo , Jin Dongsheng , Xu Minggang , Zhang Qiang
Published: July 2024

Combined organic and inorganic fertilization can improve soil fertility in coal mine reclamation areas. However, the contribution of the bacterial community (especially its occurrence patterns) to soil physicochemical properties and enzyme activity needs further evaluation. The objective of this research was to clarify the bacterial community diversity, composition, and intraspecific interactions in response to combined organic and inorganic fertilizer application in coal mine reclamation areas in the Loess Hilly Region, China. Maize rhizosphere soil samples were collected under four fertilization regimes (CK, no fertilization control; NPK, compound inorganic fertilizer; M, organic fertilizer; and NPKM, combined organic and inorganic fertilization) in a 10-year field experiment in Gujiao city, Shanxi Province. Bacterial communities were characterized using high-throughput sequencing of the 16S rRNA gene V3–V4 region. A cross-treatment Spearman correlation network was constructed to explore the bacterial co-occurrence patterns. Compared with CK, NPK, M, and NPKM decreased the pH by 0.59%, 2.27%, and 0.12%; increased the soil organic carbon by 11.25%, 11.69%, and 27.05%; and significantly decreased the bacterial Shannon diversity by 3.68%, 0.14%, and 3.54%, respectively. Compared with CK, NPKM significantly increased sucrase, urease, and alkaline phosphatase activities (p < 0.05). Critically, oligotrophic Acidobacteria were significantly more abundant in CK than in the other treatments. Gemmatimonadetes were more abundant in NPK and M, and Actinobacteria, Bacteroidota, and Patescibacteria were more abundant in NPKM. In addition, network analysis revealed that the keystone taxa in the different fertilization treatments belonged to different network modules and were significantly correlated with soil nutrient content and enzyme activity. Simultaneously, the Actinobacteria enriched in NPKM formed specific clusters through strong symbiosis, and there were significant positive correlations among sucrase, urease, and alkaline phosphatase. In summary, long-term combined organic and inorganic fertilization improved maize rhizosphere soil fertility by regulating enzyme activity, bacterial community composition, and bacterial species interactions in coal mine reclamation areas in the Loess Hilly Region.
Insights for Soil Improvements: Unraveling Distinct Mechanisms of Microbial Residue Carbon Accumulation under Chemical and Anaerobic Soil Disinfestation
Authors: Yang Kejian , Yan Jiangtao , Wang Xianwei , She Pengtao , Li Zhonghui , Xu Risheng , Chen Yanlong
Published: July 2024

Soil disinfestation has been widely used as an effective strategy to improve soil health and crop yield by suppression of soil-borne plant pathogens, but its effect on soil organic carbon (SOC), a crucial factor linked to climate change, remains unknown. A microcosm trial was conducted to evaluate microbial residue carbon (MRC) and its contribution to SOC under chemical soil disinfestation (CSD) with quicklime (QL) and chloropicrin (CP), as well as anaerobic soil disinfestation (ASD) with maize straw (MASD) and soybean straw (SASD). The SOC concentrations were increased by both CSD and ASD. Also, total SOC-normalized MRC concentration was enhanced, with a considerable increase in soil bacterial and fungal MRC, particularly evident under CP and SASD treatment. Due to broad-spectrum biocidal activities, decreased SOC-normalized microbial biomass carbon (MBC) was consistent with the reductions in bacterial and fungal phospholipid fatty acids (PLFAs), consequently increasing MRC accumulation under CSD. Similarly, ASD decreased fungal PLFAs while shifting bacterial PLFAs from aerobic to anaerobic taxa or from gram-negative to -positive taxa, both of which contributed to both MBC and MRC buildup. Collectively, the findings demonstrate that ASD can efficiently increase SOC concentration, with distinct mechanisms underlying MRC generation when compared to traditional CSD.
Tuber Development and Tuber Yield Potential of Winged Bean (Psophocarpus tetragonolobus (L.) DC.), an Alternative Crop for Animal Feed
Authors: Rakvong Teppratan , Monkham Tidarat , Sanitchon Jirawat , Chankaew Sompong
Published: July 2024

The winged bean is only one of the members of the leguminous species that has the potential for duo-purpose for its pod and tuber yields. The protein and nutritional content in the tuber can be used as alternative crops for animal feed. Previous studies identified the winged bean varieties as having high potential for tuber production, although their tuber development remains unclear. This study aims to elucidate the development of winged bean tubers based on their size and weight during eight months of growth. Tuber yields of high-potential winged bean varieties (W018 and W099) were compared to commercial varieties (Ratchaburi) under field conditions over two years. The experiments were laid out in randomized complete block design with three replications in the 2021–2022 and 2022–2023 growing seasons. The tuber formation of winged bean exhibited a significant increase in tuber weight and size in the fourth month of growth in all varieties, with a gradual change from fibrous roots to tubers. Particularly in the W018 and W099 varieties, tuber development persisted up to eight months after transplanting. On the other hand, the Ratchaburi variety had small tuber size, resulting in the tubers weighing less than in the W018 and W099 varieties. The results indicated that winged bean varieties had different tuber development, with the W018 and W099 varieties being suitable for tuber harvesting at eight months. The large tuber size can be an appropriate alternative to animal feed materials. At the same time, the Ratchaburi variety requires only five months since it is cultivated primarily for tuber consumption, which is concerned with size and shape. Moreover, the yields of the W018 and W099 varieties were significantly higher compared to Ratchaburi at 22.4, 19.3, and 7.423 T/ha in the 2021–2022 growing season and 8.4, 7.9, and 5.6 T/ha in the growing season of 2022–2023, respectively. The protein content in the winged bean tubers increased to 13.00% at eight months. The years (Y) and genotypes (G) differed considerably for tuber formation. The variation in both Y and G and the shoot/root ratio are also discussed in this study.
Effects of Micellar Quercetin Supplementation on Growth Performance, Nutrient Digestibility, Fecal Microbiota, Meat Quality, and Physiological Status in Broiler Chickens
Authors: Ahammad Golam Sagir , Kim In Ho
Published: July 2024

This study investigated the impacts of micellar quercetin (MQ) supplementation on growth performance, meat stability, excreta gas emissions, and physiological status. During a 35-day trial, 640 Ross 308 broilers were utilized. These birds were one day old, with an average initial body weight of 43.34 ± 1.43 g. They were randomly distributed across four experimental diets, each consisting of 10 replicate pens with 16 chicks per pen. The diets included the following: control (CON) with 0% micellar quercetin (MQ), TRT1 with 0.025% MQ, TRT2 with 0.050% MQ, and TRT3 with 0.100% MQ. The results indicate that broilers fed diets with increasing levels of MQ exhibited significantly higher body weight gains (BWGs) compared to the control group (p < 0.05). There was a clear linear increase in the breast muscle percentage with higher levels of quercetin supplementation (p < 0.05), while the breast color remained consistent across all groups (p > 0.05). Both cooking loss and drip loss exhibited a linear decrease as MQ levels in the diet increased (p < 0.05). The level of aspartate aminotransferase (AST) tended to decrease with higher MQ levels. Thyroxine (T4) and lymphocyte levels also showed a linear increase with increasing MQ dosage in the diet (p < 0.05). However, no significant effects were observed on nutrient digestibility, gas emissions, or fecal microbial components (Lactobacillus, E. coli, and Salmonella) with higher levels of MQ supplementation (p > 0.05). In conclusion, augmenting quercetin levels in the diet positively influenced the BWG, breast muscle development, and meat quality parameters such as cooking loss and drip loss, with beneficial effects on blood profiles.
Effects of Adding Sphingomonas Z392 to Drinking Water on Growth Performance, Intestinal Histological Structure, and Microbial Community of Broiler Chickens
Authors: Wang Mingcheng , Zhong Jie , Guo Yanan , Zhao Shuqiang , Xia Huili , Wang Gailing , Liu Chaoying , Guo Aizhen
Published: July 2024

Probiotics are a prominent alternative to antibiotics in antimicrobial-free broiler farming. To assess the effect of Sphingomonas sp. Z392 (isolated and identified) on broiler growth, 600 one-day-old Kebao broiler chickens were randomly divided into a control group and an experimental group. Each group had three replicates, with 100 broiler chickens being raised in each replicate. Regarding the experimental group of broiler chickens, 4.0 × 105 CFU/mL of Sphingomonas Z392 was added to their drinking water. Then, the changes in broiler body weight, the EPI, intestinal histological structure, and gut microbiota were examined. The results show that the supplementation of the broilers’ drinking water with 4 × 105 CFU/mL of Sphingomonas Z392 resulted in an increase in the relative abundance of Lactobacillus, Bacteroides, Lachnospiraceae, Aminobacterium, Oribacterium, Christensenellaceae, Faecalibacterium, Barnesiella, Ruminococcus, Parabacteroides, Phascolarctobacterium, Butyricicoccaceae, and Caproiciproducens, which have been reported to be positively correlated with the improved digestion and absorption of broiler chickens. The relative abundance of Odoribacter, Alistipes, Parabacteroides, and Rikenellaceae increased, and these have been reported to be negatively correlated with the occurrence of intestinal diseases. The relative abundance of Campylobacter, Shigella Castellani, Bilophila, Campylobacter, Clostridia, and Anaerotruncus decreased, and these have been reported to be positively correlated with the occurrence of intestinal diseases. At the same time, the following also increased: the integrity of small intestinal villus morphology; the number of goblet cells in small intestinal epithelial cells; the health of the mitochondria in the cytoplasm of jejunal villous epithelial cells; the number of lysosomes in the cytoplasm of goblet cells in the small intestinal epithelium, ileal villous epithelial cells, and mitochondria in the cytoplasm of large intestinal villous epithelial cells; the VH/CD of the ileum; and digestive, absorption, and defense capabilities. In particular, the final weight increased by 4.33%, and the EPI increased by 10.10%. Therefore, the supplementation of broiler drinking water with Sphingomonas generated better economic benefits from the broiler chickens.
Effects of Human Harvesting, Residences, and Forage Abundance on Deer Spatial Distribution
Authors: Takada Hayato , Nakamura Keita
Published: July 2024

It has been known that harvesting by humans strongly influences individual within-home range habitat selection of many deer species; however, little is known about the effect of harvesting on coarse-scale habitat selection (i.e., spatial distribution). We examined the summer spatial distribution of sika deer Cervus nippon in relation to human harvesting and other factors, such as human residences, forage abundance, and cover, using pellet group counts at Mount Fuji, central Japan, in 2018. In the study area, harvesting is conducted at medium elevation areas throughout the year, but not at high or low elevation areas where access is difficult or harvesting is prohibited. Spatial distribution of deer was significantly biased to non-harvesting areas and far from residential areas, suggesting that they avoid riskier spaces by establishing a landscape of fear. High-quality food resources (deciduous broad-leaved trees and forbs) were more abundant in harvesting areas than in non-harvesting areas, suggesting that foraging pressure by deer reduce them. However, there were no differences in abundances of more fibrous dwarf bamboo between harvesting and non-harvesting areas, and spatial distribution of deer was significantly biased to higher dwarf bamboo abundance areas, suggesting that the dwarf bamboo is an alternative food resource in non-harvesting areas where supplies of high-quality food were limited. Our results suggest that human harvesting pressure and residences shifted the spatial distribution of deer from the montane forests to subalpine/alpine zones, which may increase damage to vulnerable ecosystems due to severe foraging pressure.
The Impact of Streptomyces griseus Protease Reserved for Protein Evaluation of Ruminant Feed on Carbohydrase Activity during Co-Incubation
Authors: Okon Paul , Liebscher Sandra , Simon Andreas Hans , Wensch-Dorendorf Monika , Bachmann Martin , Bordusa Frank , Zeyner Annette
Published: July 2024

For protein evaluation of feedstuffs for ruminants, the Streptomyces griseus protease test provides a solely enzymatic method for estimating ruminal protein degradation. Since plant proteins are often structured in carbohydrate complexes, the use of carbohydrase during the test might improve its accuracy. It is advisable to co-incubate protease and carbohydrase, risking that the carbohydrase activity is reduced under the influence of the protease. The present study was conducted to investigate this impact by using α-amylase or the multi-enzyme complex Viscozym® L as carbohydrase. The detection of active protease was determined fluorescence photometrically using internally quenched fluorogenic substrates (IQFS). Cellulose, pectin, and starch degradation were determined spectrophotometrically using 3,5-dinitro salicylic acid as a colorimetric agent. The Streptomyces griseus protease mixture proved to be active for the selected IQFS immediately after the start of measurements (p < 0.05). Starch hydrolysis catalyzed by α-amylase or Viscozym® L, respectively, was decreased by co-incubation with protease mixture by maximal 3% or 37%, respectively, at 5 h incubation time (p > 0.05). Pectin and cellulose hydrolysis catalyzed by Viscozym® L, respectively, was not significantly influenced by co-incubation with a protease mixture (p > 0.05). Although a decrease in carbohydrase activity during co-incubation with Streptomyces griseus protease occurred, it was only numerical and might be counteracted by an adapted carbohydrase activity.
Detection of Polymorphisms in FASN, DGAT1, and PPARGC1A Genes and Their Association with Milk Yield and Composition Traits in River Buffalo of Bangladesh
Authors: Mou Monira Akter , Deb Gautam Kumar , Hridoy Md. Forhad Ahmed , Alam Md. Ashadul , Barai Hasi Rani , Haque Md Azizul , Bhuiyan Mohammad Shamsul Alam
Published: July 2024

This study aimed to identify SNPs in the intron, exon, and UTR regions of the FASN, DGAT1, and PPARGC1A genes and to investigate their possible association with milk yield and composition traits in the riverine buffalo of Bangladesh. A total of 150 DNA samples from riverine buffalo were used for PCR amplification with five pairs of primers, followed by association studies using a generalized linear model in R. SNP genotyping was performed by direct sequencing of the respective amplicon. Traits analyzed included DMY, fat%, protein%, and SNF%. This study identified 8 SNPs in FASN (g.7163G>A and g.7271C>T), DGAT1 (g.7809C>T and g.8525C>T) and PPARGC1A (g.387642C>T, g.387758A>G, g.409354A>G, and g.409452G>A). Genotypic and allelic frequencies differed significantly for each SNP genotype and did not follow the Hardy–Weinberg principle (p < 0.01 or p < 0.001) in most cases. The g.7163G>A and g.7271C>T SNP genotypes of the FASN gene were significantly associated with milk fat%, with the latter also significantly associated with SNF%. The g.8525C>T polymorphism of the DGAT1 gene significantly affected protein% (p < 0.01). Additionally, PPARGC1A gene polymorphisms showed significant associations: g.387642C>T with fat% (p < 0.05); g.387758A>G and g.409354A>G with protein% (p < 0.001) and SNF% (p < 0.01); and g.409452G>A with DMY (p < 0.001), fat% (p < 0.05), and protein% (p < 0.01). Reconstructed haplotypes of the PPARGC1A gene were significantly associated (p < 0.01) with all traits except SNF%. These findings suggest that polymorphisms in these three candidate genes have the potential as molecular markers for improving milk yield and composition traits in the riverine buffalo of Bangladesh.
Multi-Allelic Mitochondrial DNA Deletions in an Adult Dog with Chronic Weakness, Exercise Intolerance and Lactic Acidemia
Authors: Shelton G. Diane , Mickelson James R. , Friedenberg Steven G. , Cullen Jonah N. , Mehra Jaya M. , Guo Ling T. , Minor Katie M.
Published: July 2024

(1) Background: An adult dog was presented to a board-certified veterinary neurologist for evaluation of chronic weakness, exercise intolerance and lactic acidemia. (2) Methods: A mitochondrial myopathy was diagnosed based on the histological and histochemical phenotype of numerous COX-negative muscle fibers. Whole-genome sequencing established the presence of multiple extended deletions in the mitochondrial DNA (mtDNA), with the highest prevalence between the 1–11 kb positions of the approximately 16 kb mitochondrial chromosome. Such findings are typically suggestive of an underlying nuclear genome variant affecting mitochondrial replication, repair, or metabolism. (3) Results: Numerous variants in the nuclear genome unique to the case were identified in the whole-genome sequence data, and one, the insertion of a DYNLT1 retrogene, whose parent gene is a regulator of the mitochondrial voltage-dependent anion channel (VDAC), was considered a plausible causal variant. (4) Conclusions: Here, we add mitochondrial deletion disorders to the spectrum of myopathies affecting adult dogs.
Effects of Lactoferrin and Lactobacillus Supplementation on Immune Function, Oxidative Stress, and Gut Microbiota in Kittens
Authors: Dong Hao , Wang Weiwei , Chen Qianqian , Chang Xiaohan , Wang Longjiao , Chen Shuxing , Chen Lishui , Wang Ran , Ge Shaoyang , Xiong Wei
Published: July 2024

Immune deficiency is a prevalent issue among kittens, severely threatening their health and development by increasing susceptibility to infections and diseases. This study investigates the effects of dietary supplements containing lactoferrin and Lactobacillus plantarum (L. plantarum) on the immune function, intestinal health, and microbiota composition of kittens. The results demonstrate that these supplements significantly enhance immune responses, with immunoglobulin A (IgA) levels increasing by 14.9% and IgG levels by 14.2%. Additionally, there was a notable 28.7% increase in catalase activity, indicating a reduction in oxidative stress. Gastrointestinal (GI) health improved markedly, evidenced by increased populations of beneficial bacteria such as Lactobacillus, which rose from 4.13% to 79.03% over the study period. The DNC group also showed significant reductions in pro-inflammatory cytokines, including decreases of 13.94% in IL-2, 26.46% in TNF-α, and 19.45% in IFN-γ levels. Furthermore, improvements in physical conditions were observed, including enhanced coat condition and mental status. These findings underline the potential of lactoferrin and L. plantarum as effective dietary interventions to improve kitten health, thereby reducing dependency on antibiotics and mitigating associated risks. This research provides a scientific foundation for optimizing nutritional management practices to enhance the overall vitality of kittens during their critical growth phases.
Verification and Establishment of Techniques of Ajami Artwork
Authors: Baydoun Ziad , Tenku Shariman Tenku Putri Norishah , Mustaffa Fauzan
Published: July 2024

Ajami, a technique of painted wood paneling, was popular in the Ottoman Empire from the 17th to the late 18th centuries. Ajami art became prominent in Syria after the decline of tile production, and it rose to a sophisticated level of art in both local and global markets. Today, however, Ajami art has become almost forgotten and unknown by the modern generation, due to being an exclusive art that can be seen only in palaces, museums, and historical houses. This study investigates the traditional method and techniques of making Ajami, with a focus on the work of a renowned Syrian Ajami art master artisan named Mr. Abdulraouf. The study aims to identify and document the traditional method of Ajami production and determine the materials and techniques used for making Ajami. The study found that Ajami art consists of natural elements that are utilized in four main stages; foundation, design, painting, and finishing. The artisans have a strong preference for floral and geometric designs, influenced by Islamic religious beliefs. The findings of this study could serve as an educational guide to preserve heritage and make it presentable for the present and future generations.