bluuebunny/crossref_metadata_2025 huggingface dataset:
HIV-1 Integrase Strand Transfer Inhibitors and Neurodevelopment
Authors: Foster Emma G. , Gendelman Howard E. , Bade Aditya N.
Published: December 2022

Children born to mothers, with or at risk, of human immunodeficiency virus type-1 (HIV-1) infection are on the rise due to affordable access of antiretroviral therapy (ART) to pregnant women or those of childbearing age. Each year, up to 1.3 million HIV-1-infected women on ART have given birth with recorded mother-to-child HIV-1 transmission rates of less than 1%. Despite this benefit, the outcomes of children exposed to antiretroviral drugs during pregnancy, especially pre- and post- natal neurodevelopment remain incompletely understood. This is due, in part, to the fact that pregnant women are underrepresented in clinical trials. This is underscored by any potential risks of neural tube defects (NTDs) linked, in measure, to periconceptional usage of dolutegravir (DTG). A potential association between DTG and NTDs was first described in Botswana in 2018. Incidence studies of neurodevelopmental outcomes associated with DTG, and other integrase strand transfer inhibitors (INSTIs) are limited as widespread use of INSTIs has begun only recently in pregnant women. Therefore, any associations between INSTI use during pregnancy, and neurodevelopmental abnormalities remain to be explored. Herein, United States Food and Drug Administration approved ARVs and their use during pregnancy are discussed. We provide updates on INSTI pharmacokinetics and adverse events during pregnancy together with underlying mechanisms which could affect fetal neurodevelopment. Overall, this review seeks to educate both clinical and basic scientists on potential consequences of INSTIs on fetal outcomes as a foundation for future scientific investigations.
Development of a Self-Assembled Hydrogels Based on Carboxymethyl Chitosan and Oxidized Hyaluronic Acid Containing Tanshinone Extract Nanocrystals for Enhanced Dissolution and Acne Treatment
Authors: Tang Xiaohan , Liu Yan , Yuan Hailong , Gao Rong
Published: December 2022

This study aimed to construct a pH-responsive nanocrystalline hydrogel drug delivery system for topical delivery of insoluble drugs based on the self-assembly behavior of carboxymethyl chitosan (CMC) and oxidized hyaluronic acid (OHA). The tanshinone nanocrystal (TNCs) extract was prepared by dielectric milling method, the type and ratio of stabilizer of the drug were investigated to optimize the prescription, and the effector surface method was used to optimize the preparation process. OHA was prepared by the sodium periodate oxidation method, and the concentration of CMC and OHA was optimized using gel formation time as an indicator. OHA was dissolved in TNCs and self-assembled with CMC solution to form tanshinone extract nanocrystal hydrogels (CMC-OHA/TNCs), of which the physicochemical properties and in vitro antibacterial activity were evaluated. Results showed that the optimized prescription and process could produce tanshinone extract nanocrystals with a particle size of (223.67 ± 4.03) nm and a polydispersity index (PDI) of 0.2173 ± 0.0008. According to SEM and XRD results, TNCs were completely wrapped in the hydrogel as nanoparticles, and the crystallinity of TNCs was reduced and the diffraction peaks in CMC-OHA/TNCs almost disappeared. In vitro, transdermal test results showed that CMC-OHA/TNCs could release the drug continuously at the acne lesions. The cell-counting kit-8 (CCK-8) assay confirmed that the CMC-OHA/TNCs had no obvious cytotoxicity. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CMC-OHA/TNCs against Propionibacterium acnes and Staphylococcus aureus were significantly lower and the diameter of the inhibition circle was obviously higher than that of TNCs and tanshinone extract crude suspension. This study demonstrated that CMC-OHA/TNCs was a promising delivery system for topical delivery of insoluble drugs, which could improve the solubility of tanshinone extract and enhance its in vitro bacterial inhibitory activity.
Development and Evaluation of Ethosomes Loaded with Zingiber zerumbet Linn Rhizome Extract for Antifungal Skin Infection in Deep Layer Skin
Authors: Huanbutta Kampanart , Rattanachitthawat Napapat , Luangpraditkun Kunlathida , Sriamornsak Pornsak , Puri Vivek , Singh Inderbir , Sangnim Tanikan
Published: December 2022

Skin fungal infection is still a serious public health problem due to the high number of cases. Even though medicines are available for this disease, drug resistance among patients has increased. Moreover, access to medicine is restricted in some areas. One of the therapeutic options is herbal medicine. This study aims to develop an ethosome formulation loaded with Zingiber zerumbet (L.) Smith. rhizome extract for enhanced antifungal activity in deep layer skin, which is difficult to cure. Ethosomes were successfully prepared by the cold method, and the optimized formulation was composed of 1% (w/v) phosphatidylcholine and 40% (v/v) ethanol. Transmission electron microscope (TEM) images revealed that the ethosomes had a vesicle shape with a diameter of 205.6–368.5 nm. The entrapment of ethosomes was 31.58% and could inhibit the growth of Candida albicans at a concentration of 312.5 μg/mL. Finally, the ethosome system significantly enhanced the skin penetration and retention of the active compound (zerumbone) compared with the liquid extract. This study showed that Z. zerumbet (L.) rhizome extract could be loaded into ethosomes. The findings could be carried over to the next step for clinical application by conducting further in vivo penetration and permeation tests.
Pluronic® F127 Thermoresponsive Viscum album Hydrogel: Physicochemical Features and Cellular In Vitro Evaluation
Authors: Rocha Mariana S. , Batista João V. C. , Melo Michelle N. O. , Campos Vania E. B. de , Toledo Anna Lecticia M. M. , Oliveira Adriana P. , Picciani Paulo H. S. , Baumgartner Stephan , Holandino Carla
Published: December 2022

Viscum album L., popularly known as mistletoe, is well known for its anti-cancer properties, and the pharmaceutical application of hydroalcoholic dry extracts is still limited due to its low solubility in aqueous media, and physicochemical instability. The Pluronic® F127 is an amphiphilic polymer, which permits the solubilization of lipophilic and hydrophilic compounds. In this investigation, physicochemical features of hydrogel containing V. album dry extract (VADE-loaded-hydrogel) were performed by: dynamic light scattering (DLS), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and transmission electron microscopy (TEM). VADE-loaded-hydrogel presented nanometer-size micelles with volume distribution ranging from 10.58 nm to 246.7 nm, and a polydispersity index of 0.441. The sample thermal analyses (TG and DSC) showed similar decomposition curves; however, the thermal events indicated an increase in thermal stability in relation to the presence of the extract. In addition to these interesting pharmaceutical features, IC50 values of 333.40 µg/mL and >1000 µg/mL were obtained when tumor (SCC-25) and non-tumor (L929) cells were incubated with VADE-loaded-hydrogel, respectively. The optical and ultrastructural cellular analysis confirmed the tumor selectivity since the following alterations were detected only in SCC-25 cells: disorganization of plasmatic membrane; an increase of cytoplasmatic vacuole size; alteration in the cristae mitochondrial shape; and generation of amorphous cellular material. These results emphasize the promising antitumoral potential of VADE-loaded-hydrogel as an herbal drug delivery system via in vitro assays.
Light-Switchable Membrane Permeability in Giant Unilamellar Vesicles
Authors: Albanese Paola , Cataldini Simone , Ren Chloe Z.-J. , Valletti Nadia , Brunetti Jlenia , Chen Jack L.-Y. , Rossi Federico
Published: December 2022

In this work, giant unilamellar vesicles (GUVs) were synthesized by blending the natural phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) with a photoswitchable amphiphile (1) that undergoes photoisomerization upon irradiation with UV-A (E to Z) and blue (Z to E) light. The mixed vesicles showed marked changes in behavior in response to UV light, including changes in morphology and the opening of pores. The fine control of membrane permeability with consequent cargo release could be attained by modulating either the UV irradiation intensity or the membrane composition. As a proof of concept, the photocontrolled release of sucrose from mixed GUVs is demonstrated using microscopy (phase contrast) and confocal studies. The permeability of the GUVs to sucrose could be increased to ~4 × 10–2 μm/s when the system was illuminated by UV light. With respect to previously reported systems (entirely composed of synthetic amphiphiles), our findings demonstrate the potential of photosensitive GUVs that are mainly composed of natural lipids to be used in medical and biomedical applications, such as targeted drug delivery and localized topical treatments.
Tight Focusing Properties of Ring Pearcey Beams with a Cross Phase
Authors: Yu Chaoqun , He Zhimin , Huang Cheng , Chen Fuchang , Zeng Jun , Li Yan , Zhang Yongtao , Pu Jixiong , Lin Huichuan
Published: December 2022

We theoretically investigated the properties of tightly focused ring Pearcey beams with a cross phase (CPRPB). The expressions of the distributions of both electric field and magnetic field in the focal region of an objective were first derived from the vectorial Debye theory, and then numerical calculations were carried out to obtain the focused intensity distribution and the Poynting vector of CPRPB near the focus. Numerical calculations indicate that as CPRPB is focused on an objective of high numerical aperture (NA), two nonuniform self-focusing spots occur at both sides of the geometrical focus of the objective symmetrically, and the angle between their directions is 90 degrees. The stronger is the strength of cross-phase modulation, the flatter are the ellipses of the self-focusing spots, and the smaller is the intensity at the geometrical focus of the objective. Numerical calculations also demonstrate that the optical gradient force produced by tightly focused CPRPB in the focal region can be manipulated in magnitude and in direction by tuning the strength of cross-phase modulation. Due to these properties of tightly focused CPRPB, they might find applications in the manipulation of micro- and nanoparticles and so on.
After-Effects of Hydrochar Amendment on Water Spinach Production, N Leaching, and N2O Emission from a Vegetable Soil under Varying N-Inputs
Authors: Sun Haijun , Chen Ying , Yi Zhenghua
Published: December 2022

Biochar use in agriculture brings significant agronomic and environmental co-benefits, which are a function of biochar and crop types and nitrogen (N) rates. We here conducted a soil column experiment to evaluate the after-effects of hydrochar amendment at 0.5 and 2.0 wt% on vegetable production, N recovery and losses via leaching and nitrous oxide (N2O) emission from water-spinach (Ipomoea aquatica Forsk)-planted vegetable soil receiving three N inputs (120, 160, and 200 kg/ha). The results showed that hydrochar with 2.0 wt% significantly (p < 0.05) improved the biomass yield of water spinach, receiving 120–160 kg N/ha by 11.6–14.2%, compared with no change in the hydrochar treatment. Hydrochar had no effect on total N content of water spinach, and only increased the total N recovery under 2.0 wt% given hydrochar amended treatment with 120 kg N/ha. Neither pH or EC of leachate was changed with N reduction or hydrochar application. However, in some cases, hydrochar changes the NH4+, NO3− and total N concentrations in leachate. When applied at 2.0 wt%, hydrochar significantly (p < 0.05) increased total N leaching losses by 28.9% and 57.1%, under 120 and 160 kg N/ha plot, respectively. Hydrochar applied at two rates increased the N2O emissions by 109–133% under 200 kg N/ha but decreased them by 46–67% under 160 kg N/ha. Therefore, after three years of application, hydrochar still improves the production of leafy vegetable, but the impacts on N leaching and N2O emission vary, depending on inorganic N and hydrochar application rates.
PDMS/Ag/Mxene/Polyurethane Conductive Yarn as a Highly Reliable and Stretchable Strain Sensor for Human Motion Monitoring
Authors: Zhang Shichen , Xu Jiangtao
Published: December 2022

The conductivity and sensing stability of yarn-based strain sensors are still challenges when it comes to practical applications. To address these challenges, surface engineering of polyurethane (PU) yarn was introduced to improve its surface hydrophilicity for better deposition of MXene nanosheets in its dispersion. The introduction of Ag nanoparticles via magnetron sputtering greatly improved the surface conductivity; meanwhile, the encapsulation of the PDMS protective layer effectively enhanced the sensing stability over 15,000 cycling process, as well as the working range with a gauge factor value over 700 under a strain range of 150–300%. Moreover, the exploration of its applications in human motion monitoring indicate that the prepared strain-sensing yarn shows great potential in detecting both tiny motions or large-scale movements of the human body, which will be suitable for further development into multifunctional smart wearable sensors or metaverse applications in the future.
Self-Healing of Polymers and Polymer Composites
Authors: Irzhak Vadim I. , Uflyand Igor E. , Dzhardimalieva Gulzhian I.
Published: December 2022

This review is devoted to the description of methods for the self-healing of polymers, polymer composites, and coatings. The self-healing of damages that occur during the operation of the corresponding structures makes it possible to extend the service life of the latter, and in this case, the problem of saving non-renewable resources is simultaneously solved. Two strategies are considered: (a) creating reversible crosslinks in the thermoplastic and (b) introducing a healing agent into cracks. Bond exchange reactions in network polymers (a) proceed as a dissociative process, in which crosslinks are split into their constituent reactive fragments with subsequent regeneration, or as an associative process, the limiting stage of which is the interaction of the reactive end group and the crosslink. The latter process is implemented in vitrimers. Strategy (b) is associated with the use of containers (hollow glass fibers, capsules, microvessels) that burst under the action of a crack. Particular attention is paid to self-healing processes in metallopolymer systems.
Finding of Novel Galactose Utilizing Halomonas sp. YK44 for Polyhydroxybutyrate (PHB) Production
Authors: Jung Hee Ju , Kim Su Hyun , Cho Do Hyun , Kim Byung Chan , Bhatia Shashi Kant , Lee Jongbok , Jeon Jong-Min , Yoon Jeong-Jun , Yang Yung-Hun
Published: December 2022

Polyhydroxybutyrate (PHB) is a biodegradable bioplastic with potential applications as an alternative to petroleum-based plastics. However, efficient PHB production remains difficult. The main cost of PHB production is attributed to carbon sources; hence, finding inexpensive sources is important. Galactose is a possible substrate for polyhydroxyalkanoate production as it is abundant in marine environments. Marine bacteria that produce PHB from galactose could be an effective resource that can be used for efficient PHB production. In this study, to identify a galactose utilizing PHB producer, we examined 16 Halomonas strains. We demonstrated that Halomonas cerina (Halomonas sp. YK44) has the highest growth and PHB production using a culture media containing 2% galactose, final 4% NaCl, and 0.1% yeast extract. These culture conditions yielded 8.98 g/L PHB (78.1% PHB content (w/w)). When galactose-containing red algae (Eucheuma spinosum) hydrolysates were used as a carbon source, 5.2 g/L PHB was produced with 1.425% galactose after treatment with activated carbon. Since high salt conditions can be used to avoid sterilization, we examined whether Halomonas sp. YK44 could produce PHB in non-sterilized conditions. Culture media in these conditions yielded 72.41% PHB content. Thus, Halomonas sp. YK44 is robust against contamination, allowing for long-term culture and economical PHB production.
Zero–Waste Recycling of Fiber/Epoxy from Scrap Wind Turbine Blades for Effective Resource Utilization
Authors: Du Chunbao , Jin Ge , Zhang Lihui , Tong Bo , Wang Bingjia , Zhang Gang , Cheng Yuan
Published: December 2022

The number of scrap wind turbines is expanding globally as the wind power industry develops rapidly. Zero–waste recycling of scrap wind turbine blades (WTB) is the key for wind power firms to achieve green and sustainable development on the premise of satisfying environmental protection criteria. In this work, the pyrolysis of fiber/epoxy composites obtained from scrap WTB in oxidizing inert atmospheres was investigated. Various characterization methods were employed to characterize the microstructure and chemical characteristics of the heat–treated fiber/epoxy and to reveal the pyrolysis mechanism. In addition, the heat–treated fibers/epoxy were used as reinforcing agents to investigate their impact on the elastic deformation of butadiene styrene rubber–based flexible composites, and the reinforcing mechanism was revealed. The results revealed that the constituents of fiber/epoxy composites were mostly fiberglass (SiO2, CaCO3) and cured epoxy resin, with covalent bonding being the interaction between the fiberglass and epoxy resin. The total weight of the epoxy resin in the fiber/epoxy composites was 22%, and the 11% weight loss was achieved at around 350 °C, regardless of the presence of oxygen; however, the features of heat–treated fibers/epoxy were associated with the pyrolysis atmosphere at a higher temperature. The pyrolysis products in inert atmospheres, with water contact angles of 58.8°, can considerably improve the tensile properties of flexible composites at the elastic stage. Furthermore, the flexible composite granules were prepared to plug large channels in sand–filled pipes, and the plugging rate had the potential to reach 81.1% with an injection volume of 5.0 PV. The plugging performance was essentially unaffected by water salinity, owing to the high stability of flexible composite granules in mineralized water. The findings of this study present a realistic route to the industrial application of fiber/epoxy, as well as a novel approach for encouraging the efficient use of scrap wind turbines on a large scale.
The Injected Foaming Study of Polypropylene/Multiwall Carbon Nanotube Composite with In Situ Fibrillation Reinforcement
Authors: Li Gang , Fei Yanpei , Kuang Tairong , Liu Tong , Zhong Mingqiang , Li Yanbiao , Jiang Jing , Turng Lih-Sheng , Chen Feng
Published: December 2022

This paper explored the injection foaming process of in situ fibrillation reinforced polypropylene composites. Using polypropylene (PP) as the continuous phase, polytetrafluoroethylene (PTFE) as the dispersed phase, multi–wall carbon nanotubes (MWCNTs) as the conductive filler, and PP grafted with maleic anhydride (PP–g–MA) as the compatibilizer, a MWCNTs/PP–g–MA masterbatch was prepared by using a solution blending method. Then, a lightweight, conductive PP/PTFE/MWCNTs composite foam was prepared by means of extruder granulation and supercritical nitrogen (ScN2) injection foaming. The composite foams were studied in terms of rheology, morphological, foaming behavior and mechanical properties. The results proved that the in situ fibrillation of PTFE can have a remarkable effect on melt strength and viscoelasticity, thus improving the foaming performance; we found that PP/3% PTFE showed excellent performance. Meanwhile, the addition of MWCNTs endows the material with conductive properties, and the conductivity reached was 2.73 × 10−5 S/m with the addition of 0.2 wt% MWCNTs. This study’s findings are expected to be applied in the lightweight, antistatic and high–performance automotive industry.
The Mechanical, Thermal, and Chemical Properties of PLA-Mg Filaments Produced via a Colloidal Route for Fused-Filament Fabrication
Authors: Orellana-Barrasa Jaime , Ferrández-Montero Ana , Boccaccini Aldo. R. , Ferrari Begoña , Pastor José Ygnacio
Published: December 2022

The effect of Mg particles on the thermal, chemical, physical, and primarily mechanical properties of 3D-printed PLA/Mg composites is studied in this paper. Recently, new colloidal processing has been proposed to introduce Mg particles into the PLA matrix, which ensures good dispersion of the particles and better thermal properties, allowing for thermal processing routes such as extrusion or 3D printing via fused-filament fabrication. The thermal and physical properties are here studied in 1D single-filament-printed PLA/Mg composites with 0 to 10 wt.% of Mg particles by Differential Scanning Calorimetry (DSC); we analyse the PLA chain modifications produced, the crystallinity fraction, and the different crystalline forms of the PLA after thermal processing. Fourier Transform Infrared Spectroscopy (FTIR) is used to confirm the influence of the PLA/Mg colloidal processing after printing. The mechanical properties are measured with a universal tensile test machine on the 1D single-printed filaments via fused-filament fabrication (FFF); the filaments were naturally aged to stable conditions. Filaments with and without a notch are studied to obtain the materials’ tensile strength, elastic modulus, and fracture toughness. Different analytical models to explain the results of the PLA-Mg were studied, in which the minimum values for the interface strength of the PLA-Mg composites were calculated.
Removal of Cationic Dyes by Iron Modified Silica/Polyurethane Composite: Kinetic, Isotherm and Thermodynamic Analyses, and Regeneration via Advanced Oxidation Process
Authors: Ahmad Afiqah , Jamil Siti Nurul Ain Md. , Choong Thomas S. Y. , Abdullah Abdul Halim , Faujan Nur Hana , Adeyi Abel A. , Daik Rusli , Othman Nurhanisah
Published: December 2022

Emerging dye pollution from textile industrial effluents is becoming more challenging for researchers worldwide. The contamination of water by dye effluents affects the living organisms in an ecosystem. Methylene blue (MB) and malachite green (MG) are soluble dyes with a high colour intensity even at low concentration and are hazardous to living organisms. The adsorption method is used in most wastewater plants for the removal of organic pollutants as it is cost-effective, has a high adsorption capacity, and good mechanical stabilities. In this study, a composite adsorbent was prepared by impregnating iron modified silica (FMS) onto polyurethane (PU) foam to produce an iron modified silica/polyurethane (FMS/PU) composite. The composite adsorbent was utilised in batch adsorption of the cationic dyes MB and MG. The effect of adsorption parameters such as the adsorbent load, pH, initial dye concentration, and contact time were discussed. Adsorption kinetics and isotherm were implemented to understand the adsorption mechanism for both dyes. It was found that the adsorption of MB and MG followed the pseudo-second order model. The Langmuir model showed a better fit than the Freundlich model for the adsorption of MB and MG, indicating that the adsorption occurred via the monolayer adsorption system. The maximum adsorption capacity of the FMS/PU obtained for MB was 31.7 mg/g, while for MG, it was 34.3 mg/g. The thermodynamic study revealed that the adsorption of MB and MG were exothermic and spontaneous at room temperature. In addition, the regeneration of FMS/PU was conducted to investigate the composite efficiency in adsorbing dyes for several cycles. The results showed that the FMS/PU composite could be regenerated up to four times when the regeneration efficiency dropped drastically to less than 20.0%. The impregnation of FMS onto PU foam also minimised the adsorbent loss into the environment.
Study on Mechanical Properties of Polyurethane Cross-Linked P(E-co-T)/PEG Blended Polyether Elastomer
Authors: Jin Puyu , Pang Aimin , Yang Rongjie , Guo Xiaoyan , He Jiyu , Zhai Jinxian
Published: December 2022

To improve the mechanical properties of polyurethane cross-linked poly (ethylene oxide-co-tetrahydrofuran) (P(E-co-T)) elastomers at room temperature, using poly (ethylene oxide-co-tetrahydrofuran) and high-molecular-weight polyethylene glycol (PEG) as raw materials and polyisocyanate N100 as curing agent, a series of polyurethane cross-linked blended polyether elastomers were prepared by changing the elastomer-curing parameter R value (n(-NCO)/n(-OH)) and P(E-co-T)/PEG ratio. Equilibrium swelling measurements showed that the chemical cross-linkage of the elastomers tended to decrease with the decreasing R value, the average molecular weight (Mc) of the network chain increased, and the density of the network chain (N0) decreased. Wide-angle X-ray diffraction (WAXD) and differential scanning calorimetry (DSC) tests showed that PEG chain segments within the elastomers crystallized at room temperature, while the crystallinity increased with decreasing R value and increasing PEG content. The mechanical property tests showed that the elongation at break tended to decrease with increasing R value; the tensile strength first increased and then decreased. At R value 0.9, the elastomer presented good comprehensive mechanical properties. In addition, the mechanical properties of polyurethane cross-linked P(E-co-T)/PEG blended polyether elastomer showed an increasing trend with the increase in PEG content when the curing parameter of 0.9 remained unchanged.
Rapid Selective Detection and Quantification of β-Blockers Used in Doping Based on Molecularly Imprinted Nanoparticles (NanoMIPs)
Authors: Cáceres César , del Pilar Garcia Morgado Macarena , Bozo Freddy Celis , Piletsky Sergey , Moczko Ewa
Published: December 2022

Human performance enhancing drugs (PEDs), frequently used in sport competitions, are strictly prohibited by the World Anti-Doping Agency (WADA). Biological samples collected from athletes and regular patients are continuously tested regarding the identification and/or quantification of the banned substances. Current work is focused on the application of a new analytical method, molecularly imprinted nanoparticles (nanoMIPs), to detect and determine concentrations of certain prohibited drugs, such as β-blockers, in water and human urine samples. These medications are used in the treatment of cardiovascular conditions, negative effects of adrenaline (helping to relief stress), and hypertension (slowing down the pulse and softening the arteries). They can also significantly increase muscle relaxation and improve heart efficiency. The new method of the detection and quantification of β-blockers is based on synthesis, characterization, and implementation of nanoMIPs (so-called plastic antibodies). It offers numerous advantages over the traditional methods, including high binding capacity, affinity, and selectivity for target molecules. Additionally, the whole process is less complicated, cheaper, and better controlled. The size and shape of the nanoMIPs is evaluated by dynamic light scattering (DLS) and transmission electron microscope (TEM). The affinity and selectivity of the nanoparticles are investigated by competitive pseudo enzyme-linked immunosorbent assay (pseudo-ELISA) similar to common immunoassays employing natural antibodies. To provide reliable results towards either doping detection or therapeutic monitoring using the minimal invasive method, the qualitative and quantitative analysis of these drugs is performed in water and human urine samples. It is demonstrated that the assay can detect β-blockers in water within the linear range 1 nmol·L−1–1 mmol·L−1 for atenolol with the detection limit 50.6 ng mL−1, and the linear range 1 mmol·L−1–10 mmol·L−1 for labetalol with the detection limit of 90.5 ng·mL−1. In human urine samples, the linear range is recorded in the concentration range 0.1 mmol·L−1–10 nmol·L−1 for atenolol and 1 mmol·L−1–10 nmol·L−1 for labetalol with a detection limit of 61.0 ng·mL−1 for atenolol and 99.4 ng·mL−1 for labetalol.
A Review of Cyclodextrin Encapsulation and Intelligent Response for the Release of Curcumin
Authors: Li Jing , Xu Fang , Dai Yujie , Zhang Jiawen , Shi Yuan , Lai Danning , Sriboonvorakul Natthida , Hu Jiamiao
Published: December 2022

To overcome the low water solubility and low bioavailability of curcumin (CUR), multiple delivery strategies have been proposed. Among these, cyclodextrin-based carriers have been widely used for the encapsulation and delivery of CUR. Cyclodextrins (CDs), as natural oligosaccharides, have been well known for their biodegradability, biocompatibility, non-toxicity, and internal hydrophobic and external hydrophilic structural features. This paper summarizes the recently reported CD-based carriers for encapsulating CUR. Particularly, the polymerization properties of CD self-assembly to enhance the encapsulation of CUR are discussed. In addition, the current progress on stimuli-responsive CD carriers for controlled release of CUR is described, which laid an important foundation for the development of CUR-based precision therapy in clinical practice. In conclusion, this review may provide ideas for the future development of a CD-based encapsulant for CUR.
Synthesis of CuO and PAA-Regulated Silver-Carried CuO Nanosheet Composites and Their Antibacterial Properties
Authors: Ni Zhihui , Wan Menghui , Tang Gongming , Sun Lei
Published: December 2022

With the aid of a facile and green aqueous solution approach, a variety of copper oxide (CuO) with different shapes and polyacrylic-acid (PAA)-regulated silver-carried CuO (CuO@Ag) nanosheet composites have been successfully produced. The point of this article was to propose a common synergy using Ag-carried CuO nanosheet composites for their potential antibacterial efficiency against three types of bacteria such as E. coli, P. aeruginosa, and S. aureus. By using various technical means such as XRD, SEM, and TEM, the morphology and composition of CuO and CuO@Ag were characterized. It was shown that both CuO and CuO@Ag have a laminar structure and exhibit good crystallization, and that the copper source and reaction duration have a sizable impact on the morphology and size distribution of the product. In the process of synthesizing CuO@Ag, the appropriate amount of polyacrylic acid (PAA) can inhibit the agglomeration of Ag NPs and regulate the size of Ag at about ten nanometers. In addition, broth dilution, optical density (OD 600), and electron microscopy analysis were used to assess the antimicrobial activity of CuO@Ag against the above three types of bacteria. CuO@Ag exhibits excellent synergistic and antibacterial action, particularly against S. aureus. The antimicrobial mechanism of the CuO@Ag nanosheet composites can be attributed to the destruction of the bacterial cell membrane and the consequent leakage of the cytoplasm by the release of Ag+ and Cu2+. The breakdown of the bacterial cell membrane and subsequent leakage of cytoplasm caused by Ag+ and Cu2+ released from antimicrobial agents may be the cause of the CuO@Ag nanosheet composites’ antibacterial action. This study shows that CuO@Ag nanosheet composites have good antibacterial properties, which also provides the basis and ideas for the application research of other silver nanocomposites.
Tannin-Epoxidized Soybean Oil as Bio-Based Resin for Fabrication of Grinding Wheel
Authors: Wang Weicong , Zhou Yunxia , Liu Bowen , Essawy Hisham , Liu Zhiyan , Deng Shuduan , Zhou Xiaojian , Zhang Jun
Published: December 2022

Formaldehyde-free epoxidized soybean oil-based resin has been prepared under acidic conditions by co-condensation of the epoxidized soybean oil and condensed tannin originating from agricultural and forestry sources as the main raw materials, whereas 1,6-hexanediamine was employed as a cross-linking agent. Fourier transform infrared spectroscopy (FTIR) and electrospray ionization (ESI) corroborated that tannin and epoxidized soybean oil underwent crosslinking under acidic conditions supported by hexamethylenediamine. A bio-based grinding wheel was fabricated by formulation of the developed resin with wood powder as source of grinding particles. The appearance, hardness, compressive strength and wear resistance of the resulting grinding wheel were studied. The results have shown that the grinding wheel possesses a smooth surface with no bubbles or cracks, and its hardness and wear resistance were greater than that of a phenolic resin-based grinding wheel. Interestingly, the grinding wheel incorporates more than 90% of its raw materials as biomass renewable materials; thus, it is generally considered non-toxic. In addition, the future feasibility of this approach to replace some petrochemical resins that are frequently used in the fabrication of grinding wheels is considered.
A Novel Approach for the Manufacturing of Gelatin-Methacryloyl
Authors: Grijalva Garces David , Radtke Carsten Philipp , Hubbuch Jürgen
Published: December 2022

Gelatin and its derivatives contain cell adhesion moieties as well as sites that enable proteolytic degradation, thus allowing cellular proliferation and migration. The processing of gelatin to its derivatives and/or gelatin-containing products is challenged by its gelation below 30 ∘C. In this study, a novel strategy was developed for the dissolution and subsequent modification of gelatin to its derivative gelatin-methacryloyl (GelMA). This approach was based on the presence of urea in the buffer media, which enabled the processing at room temperature, i.e., lower than the sol–gel transition point of the gelatin solutions. The degree of functionalization was controlled by the ratio of reactant volume to the gelatin concentration. Hydrogels with tailored mechanical properties were produced by variations of the GelMA concentration and its degree of functionalization. Moreover, the biocompatibility of hydrogels was assessed and compared to hydrogels formulated with GelMA produced by the conventional method. NIH 3T3 fibroblasts were seeded onto hydrogels and the viability showed no difference from the control after a three-day incubation period.
Holographic Lens Resolution Using the Convolution Theorem
Authors: Lloret Tomás , Morales-Vidal Marta , Navarro-Fuster Víctor , G. Ramírez Manuel , Beléndez Augusto , Pascual Inmaculada
Published: December 2022

The similarity between object and image of negative asymmetrical holographic lenses (HLs) stored in a low-toxicity photopolymer has been evaluated theoretically and experimentally. Asymmetrical experimental setups with negative focal lengths have been used to obtain HLs. For this purpose, the resolution of the HLs was calculated using the convolution theorem. A USAF 1951 test was used as an object and the impulse responses of the HLs, which in this case was the amplitude spread function (ASF), were obtained with two different methods: using a CCD sensor and a Hartmann Shack (HS) wavefront sensor. For a negative asymmetrically recorded HL a maximum resolution of 11.31 lp/mm was obtained. It was evaluated at 473 nm wavelength. A theoretical study of object-image similarity had carried out using the MSE (mean squared error) metric to evaluate the experimental results obtained quantitatively.
Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor
Authors: Rojas-Rojas Laura , Espinoza-Álvarez María Laura , Castro-Piedra Silvia , Ulloa-Fernández Andrea , Vargas-Segura Walter , Guillén-Girón Teodolito
Published: December 2022

Tissue engineering aims to develop in-vitro substitutes of native tissues. One approach of tissue engineering relies on using bioreactors combined with biomimetic scaffolds to produce study models or in-vitro substitutes. Bioreactors provide control over environmental parameters, place and hold a scaffold under desired characteristics, and apply mechanical stimulation to scaffolds. Polymers are often used for fabricating tissue-engineering scaffolds. In this study, polycaprolactone (PCL) collagen-coated microfilament scaffolds were cell-seeded with C2C12 myoblasts; then, these were grown inside a custom-built bioreactor. Cell attachment and proliferation on the scaffolds were investigated. A loading pattern was used for mechanical stimulation of the cell-seeded scaffolds. Results showed that the microfilaments provided a suitable scaffold for myoblast anchorage and that the custom-built bioreactor provided a qualified environment for the survival of the myoblasts on the polymeric scaffold. This PCL-based microfilament scaffold located inside the bioreactor proved to be a promising structure for the study of skeletal muscle models and can be used for mechanical stimulation studies in tissue engineering applications.
A Review on Biomedical Application of Polysaccharide-Based Hydrogels with a Focus on Drug Delivery Systems
Authors: Farasati Far Bahareh , Naimi-Jamal Mohammad Reza , Safaei Maryam , Zarei Kimia , Moradi Marzieh , Yazdani Nezhad Hamed
Published: December 2022

Over the last years of research on drug delivery systems (DDSs), natural polymer-based hydrogels have shown many scientific advances due to their intrinsic properties and a wide variety of potential applications. While drug efficacy and cytotoxicity play a key role, adopting a proper DDS is crucial to preserve the drug along the route of administration and possess desired therapeutic effect at the targeted site. Thus, drug delivery technology can be used to overcome the difficulties of maintaining drugs at a physiologically related serum concentration for prolonged periods. Due to their outstanding biocompatibility, polysaccharides have been thoroughly researched as a biological material for DDS advancement. To formulate a modified DDS, polysaccharides can cross-link with different molecules, resulting in hydrogels. According to our recent findings, targeted drug delivery at a certain spot occurs due to external stimulation such as temperature, pH, glucose, or light. As an adjustable biomedical device, the hydrogel has tremendous potential for nanotech applications in involved health areas such as pharmaceutical and biomedical engineering. An overview of hydrogel characteristics and functionalities is provided in this review. We focus on discussing the various kinds of hydrogel-based systems on their potential for effectively delivering drugs that are made of polysaccharides.
Adjustable Sound Absorber of Multiple Parallel-Connection Helmholtz Resonators with Tunable Apertures Prepared by Low-Force Stereolithography of Photopolymer Resin
Authors: Yang Fei , Bi Shaohua , Shen Xinmin , Li Zhizhong , Zhang Xiangpo , Wang Enshuai , Yang Xiaocui , Peng Wenqiang , Huang Changchuang , Liang Peng , Sun Guoxin
Published: December 2022

The variable noise spectrum for many actual application scenarios requires a sound absorber to adapt to this variation. An adjustable sound absorber of multiple parallel-connection Helmholtz resonators with tunable apertures (TA–MPCHRs) is prepared by the low-force stereolithography of photopolymer resin, which aims to improve the applicability of the proposed sound absorber for noise with various frequency ranges. The proposed TA–MPCHR metamaterial contains five metamaterial cells. Each metamaterial cell contains nine single Helmholtz resonators. It is treated as a basic structural unit for an array arrangement. The tunable aperture is realized by utilizing four segments of extendable cylindrical chambers with length l0, which indicates that the length of the aperture l is in the range of [l0, 4l0], and that it is tunable. With a certain group of specific parameters for the proposed TA–MPCHR, the influence of the tunable aperture with a variable length is investigated by acoustic finite element simulation with a two-dimensional rotational symmetric model. For the given noise spectrum of certain actual equipment with four operating modes, the TA–MPCHR sample with a limited total thickness of 40 mm is optimized, which is made of photopolymer resin by the low-force stereolithography, and its actual average sound absorption coefficients for the frequency ranges of 500–800 Hz, 550–900 Hz, 600–1000 Hz and 700–1150 Hz reach 0.9203, 0.9202, 0.9436 and 0.9561, respectively. Relative to common non-adjustable metamaterials, the TA–MPCHR made of photopolymer resin can reduce occupied space and improve absorption efficiency, which is favorable in promoting its practical applications in the noise pollution prevention.
Civilizational Populism in Indonesia: The Case of Front Pembela Islam (FPI)
Authors: Yilmaz Ihsan , Morieson Nicholas , Bachtiar Hasnan
Published: December 2022

This article examines whether a ‘civilizational turn’ has occurred among populist movements in Indonesia. It focuses on the civilizational elements in the populist discourse of the Front Pembela Islam (Islamic Defender Front/FPI) in Indonesia. The article traces the FPI’s history and growing influence on politics and society in Indonesia in the 2010s. This article argues that the FPI has instrumentalized religious discourse, and through it divided Indonesian society into three groups: the virtuous ummah, corrupt elites, and immoral internal and external non-Muslim enemies, especially the civilizational bloc ‘the West’. This instrumentalization gained the group a degree of popularity in the second decade of the post-Suharto period and strengthened its political power and ability to bargain with mainstream political parties. The article uses the FPI’s actions and discourse during the Ahok affair to demonstrate the civilizational turn in Indonesian populism. The article shows how the FPI grew in power during the Ahok affair, in which a Christian Chinese politician, Basuki Tjahaja Purnama, was accused of blasphemy by Indonesian Islamists and later convicted on the same charge by an Indonesian court. The FPI was a leading part of a broad coalition of Islamist groups and individuals which called for Ahok to be charged with blasphemy; charges which were eventually laid and which led to Ahok being sentenced to two years imprisonment. The FPI, the article shows, framed Ahok as a non-Muslim Christian and therefore a ‘foreign’ enemy who was spreading moral corruption in Indonesia, governing ‘elites’ as complacent in combating immorality and positioned themselves as defenders of ‘the people’ or ummah. From the security perspective of the state, the FPI presented a critical threat that required containing. As a result of the growing power of the group, the FPI was banned in 2020 and Rizieq was imprisoned, while Ahok was politically rehabilitated by the Widodo government. Although the FPI’s banning is considered the most effective nonpermanent solution for the state, there is evidence that the FPI’s discourse has been adopted by mainstream political actors. This article, then, finds that the growth of the FPI during the second decade of the post-Suharto period, and their actions in leading the persecution of Ahok, demonstrates a civilizational turn in Indonesian Islamist populism.
Original Sin, or Other Opposition to Optimism? How Harkness Differs from Wesley in the Face of Human Depravity
Authors: Cherry Natalya A.
Published: December 2022

Responding to the too-optimistic theology of fellow Arminian, John Taylor, John Wesley wrote his lengthy treatise on the doctrine of original sin. In an optimistic effort to make fellow personalist theologians’ works accessible, Methodist theologian Georgia Harkness tersely disdained the same doctrine in her first major book. She soon found her liberal theology “chastened” by interactions with neo-orthodox opponents and experiences of depravity—in world events and gender-based discrimination reflecting systemic sin. This article examines her later works for evidence of whether Harkness modified her attitude toward original sin and innovations she made to accommodate both her disdain for the doctrine and the realities of depravity.
A High-Quality Instance-Segmentation Network for Floating-Algae Detection Using RGB Images
Authors: Zou Yibo , Wang Xiaoliang , Wang Lei , Chen Ke , Ge Yan , Zhao Linlin
Published: December 2022

Floating-algae detection plays an important role in marine-pollution monitoring. The surveillance cameras on ships and shores provide a powerful way of monitoring floating macroalgae. However, the previous methods cannot effectively solve the challenging problem of detecting Ulva prolifera and Sargassum, due to many factors, such as strong interference with the marine environment and the drastic change of scale. Recently, the instance-segmentation methods based on deep learning have been successfully applied to many image-recognition tasks. In this paper, a novel instance-segmentation network named AlgaeFiner is proposed for high-quality floating-algae detection using RGB images from surveillance cameras. For improving the robustness of the model in complex ocean scenes, the CA-ResNet is firstly proposed by integrating coordinate attention into the ResNet structure to model both the channel- and position-dependencies. Meanwhile, the Ms-BiFPN is proposed by embedding the multi-scale module into the architecture of BiFPN to strengthen the ability of feature fusion at different levels. To improve the quality of floating-algae segmentation, the Mask Transfiner network is introduced into the AlgaeFiner to obtain the high-quality segmentation results. Experimental results demonstrate that the AlgaeFiner can achieve better performance on floating-algae segmentation than other state-of-the-art instance-segmentation methods, and has high application-value in the field of floating-macroalgae monitoring.
Mapping Arctic Sea-Ice Surface Roughness with Multi-Angle Imaging SpectroRadiometer
Authors: Johnson Thomas , Tsamados Michel , Muller Jan-Peter , Stroeve Julienne
Published: December 2022

Sea-ice surface roughness (SIR) is a crucial parameter in climate and oceanographic studies, constraining momentum transfer between the atmosphere and ocean, providing preconditioning for summer-melt pond extent, and being related to ice age and thickness. High-resolution roughness estimates from airborne laser measurements are limited in spatial and temporal coverage while pan-Arctic satellite roughness does not extend over multi-decadal timescales. Launched on the Terra satellite in 1999, the NASA Multi-angle Imaging SpectroRadiometer (MISR) instrument acquires optical imagery from nine near-simultaneous camera view zenith angles. Extending on previous work to model surface roughness from specular anisotropy, a training dataset of cloud-free angular reflectance signatures and surface roughness, defined as the standard deviation of the within-pixel lidar elevations, from near-coincident operation IceBridge (OIB) airborne laser data is generated and is modelled using support vector regression (SVR) with a radial basis function (RBF) kernel selected. Blocked k-fold cross-validation is implemented to tune hyperparameters using grid optimisation and to assess model performance, with an R2 (coefficient of determination) of 0.43 and MAE (mean absolute error) of 0.041 m. Product performance is assessed through independent validation by comparison with unseen similarly generated surface-roughness characterisations from pre-IceBridge missions (Pearson’s r averaged over six scenes, r = 0.58, p < 0.005), and with AWI CS2-SMOS sea-ice thickness (Spearman’s rank, rs = 0.66, p < 0.001), a known roughness proxy. We present a derived sea-ice roughness product at 1.1 km resolution (2000–2020) over the seasonal period of OIB operation and a corresponding time-series analysis. Both our instantaneous swaths and pan-Arctic monthly mosaics show considerable potential in detecting surface-ice characteristics such as deformed rough ice, thin refrozen leads, and polynyas.