bluuebunny/crossref_metadata_2025 huggingface dataset:
Salivary IL-6 mRNA is a Robust Biomarker in Oral Squamous Cell Carcinoma
Authors: Márton Ildikó Judit , Horváth József , Lábiscsák Péter , Márkus Bernadett , Dezső Balázs , Szabó Adrienn , Tar Ildikó , Piffkó József , Jakus Petra , Barabás József , Barabás Péter , Olasz Lajos , Kövér Zsanett , Tőzsér József , Sándor János , Csősz Éva , Scholtz Beáta , Kiss Csongor
Published: November 2019

Salivary IL-6 mRNA was previously identified as a promising biomarker of oral squamous cell carcinoma (OSCC). We performed a multi-center investigation covering all geographic areas of Hungary. Saliva from 95 patients with OSCC and 80 controls, all Caucasian, were collected together with demographic and clinicopathological data. Salivary IL-6 mRNA was quantified by real-time quantitative PCR. Salivary IL-6 protein concentration was measured by enzyme-linked immune-sorbent assay. IL-6 protein expression in tumor samples was investigated by immunohistochemistry. Normalized salivary IL-6 mRNA expression values were significantly higher (p < 0.001) in patients with OSCC (mean ± SE: 3.301 ± 0.885) vs. controls (mean ± SE: 0.037 ± 0.012). Differences remained significant regardless of tumor stage and grade. AUC of the ROC curve was 0.9379 (p < 0.001; 95% confidence interval: 0.8973–0.9795; sensitivity: 0.945; specificity: 0.819). Salivary IL-6 protein levels were significantly higher (p < 0.001) in patients (mean ± SE: 70.98 ± 14.06 pg/mL), than in controls (mean ± SE: 12.45 ± 3.29). Specificity and sensitivity of IL-6 protein were less favorable than that of IL-6 mRNA. Salivary IL-6 mRNA expression was significantly associated with age and dental status. IL-6 manifestation was detected in tumor cells and tumor-infiltrating leukocytes, suggesting the presence of a paracrine loop of stimulation. Salivary IL-6 mRNA is one of the best performing and clinically relevant biomarkers of OSCC.
Boundary Characteristic Bernstein Polynomials Based Solution for Free Vibration of Euler Nanobeams
Authors: Karmakar Somnath , Chakraverty Snehashish
Published: November 2019

This paper is concerned with the free vibration problem of nanobeams based on Euler–Bernoulli beam theory. The governing equations for the vibration of Euler nanobeams are considered based on Eringen’s nonlocal elasticity theory. In this investigation, computationally efficient Bernstein polynomials have been used as shape functions in the Rayleigh-Ritz method. It is worth mentioning that Bernstein polynomials make the computation efficient to obtain the frequency parameters. Different classical boundary conditions are considered to address the titled problem. Convergence of frequency parameters is also tested by increasing the number of Bernstein polynomials in the simulation. Further, comparison studies of the results with existing literature are done after fixing the number of polynomials required from the said convergence study. This shows the efficacy and powerfulness of the method. The novelty of using the Bernstein polynomials is addressed in detail and solutions obtained by this method provide a better representation of the vibration behavior of Euler nanobeams.
Three-Dimensional Numerical Simulations of Buoyant Jets Discharged from a Rosette-Type Multiport Diffuser
Authors: Yan Xiaohui , Mohammadian Abdolmajid , Chen Xin
Published: November 2019

In some outfall systems, wastewaters are discharged into ambient water bodies using rosette-type diffusers in the form of multiple buoyant jets, and it is essential to simulate their mixing characteristics for practical applications and optimal design purposes. The mixing processes of a rosette jet group are more complicated than single jets and multiple horizontal or vertical jets, and thus the existing methods cannot be effectively used to simulate their mixing and dilution properties. With the recent advancements in numerical modeling approaches, numerical simulation of wastewater jets as three-dimensional phenomena can be feasible. The present study deals with a fully three-dimensional numerical simulation for buoyant jets discharged from a rosette-type multiport diffuser, with the standard and re-normalization group (RNG) k-ε turbulence models. The simulated results are compared with experimental data, and the results show a good agreement with the experimental data, demonstrating that the numerical model is an efficient and effective tool for simulating rosette jet groups. It was also concluded that the RNG k-ε model leads to better results than the standard k-ε model with a comparable computational cost. The validated model was further utilized to investigate the influences of port inclinations on the mixing behaviors.
Modeling the Impact of Extreme River Discharge on the Nutrient Dynamics and Dissolved Oxygen in Two Adjacent Estuaries (Portugal)
Authors: Oliveira Vítor H. , Sousa Magda C. , Morgado Fernando , Dias João M.
Published: November 2019

The Minho and Lima are adjacent estuaries located in the north of Portugal, with high ecological and economic importance. To address gaps in knowledge about changes in nutrient patterns in adjacent estuaries subject to different freshwater inflows, a numerical model, Delft3D, was implemented and developed, using a single domain, which allowed physical communication between estuaries. Calibration and validation of the model was successfully performed. Three numerical simulations were carried out, in which only river flows were varied (1st corresponds to a baseline numerical run, the 2nd a flood scenario, and the 3rd a drought scenario). Under flooding conditions, similar patterns were verified in both estuaries, with high fluvial discharges showing to have a reduced impact on both estuarine dynamics. In this case the nutrients were not a limiting factor for the biota, both for summer and winter seasons, since there was no significant decrease in dissolved oxygen concentration. For the drought scenario, it was observed that the estuary with the lower inflow of freshwater (Lima) was the most affected, with a significant decrease in the concentration of nutrients and oxygen dissolved in the winter season (decrease of 2 mg O2/L). In conclusion, this work reveals that it is essential to continuously monitor dam-controlled estuarine systems, as a significant decrease in river discharge will cause significant changes in the variables analysed (O2, PO4, and NO3) and may cause loss of biodiversity.
Nearshore Dynamics of Storm Surges and Waves Induced by the 2018 Typhoons Jebi and Trami Based on the Analysis of Video Footage Recorded on the Coasts of Wakayama, Japan
Authors: Yamanaka Yusuke , Matsuba Yoshinao , Tajima Yoshimitsu , Shibata Ryotaro , Hattori Naohiro , Wu Lianhui , Okami Naoko
Published: November 2019

In this study, field surveys along the coasts of Wakayama Prefecture, Japan, were first conducted to investigate the coastal damage due to storm surges and storm-induced waves caused by the 2018 Typhoons Jebi and Trami. Special focus was placed on the characteristic behavior of nearshore waves through investigation of observed data, numerical simulations, and image analysis of video footage recorded on the coasts. The survey results indicated that inundation, wave overtopping, and drift debris caused by violent storm-induced waves were the dominant factors causing coastal damage. Results of numerical simulations showed that heights of storm-induced waves were predominantly greater than storm surge heights along the entire coast of Wakayama in both typhoons. However, computed gradual alongshore variations in wave and surge heights did not explain locally-concentrated inundation and run-up heights observed along the coasts. These results indicate that complex nearshore hydrodynamics induced by local nearshore bathymetry might have played a significant role in inducing such local wave characteristics and the associated coastal damage. Analysis of video footage recorded during Typhoon Jebi, for example, clearly showed evidence of amplified infragravity wave components, which could enhance inundation and wave run-up.
The Effect of Rare Earths Additions on the Microstructure and the Corrosion Behavior of Sn-0.7Cu-0.075Al Solder Alloy
Authors: Yang Wenchao , Du Zaixiang , Yu Shuyuan , Li Yitai , Feng Junli , Wei Xuanchen , Li Qiang , Zhan Yongzhong
Published: November 2019

Sn-0.7Cu-0.075Al solder alloy adding with Ce and La had been successfully prepared by applying ball-milling and vacuum arc remelting. The influence of Ce and La on microstructure and corrosion behavior of Sn-0.7Cu-0.075Al solder alloy was investigated. The results showed that Ce (La)-containing solders had refined grains and obvious directional tendency due to the dispersive refiner (CeO2 and La2O3). Electrochemical potentiodynamic curves revealed three different stages of the reaction, including anodic and cathodic processes, prepassivation section, and stable passivation stages. The self-corrosion potential (Ecorr) of alloys with Ce and La addition were a little bit more negative, hardly making a difference on corrosion occurrence. However, the corrosion current density (Icorr) and passivation current density (Ip) decreased by two-thirds and one-half respectively, which indicated a better corrosion resistant after adding rare earths. The recorded micrographs of corroded surface at different polarized points witnessed the formation of corrosion product film both on prepassivation and passivation stage. Moreover, the cross section of corrosion product film showed the coarse, loose film in Sn-0.7Cu-0.075Al solder and adherent, compact film in Ce (La)-containing solders, which further indicated an excellent anti-corrosion property.
Sol–Gel Synthesis and Characterization of Coatings of Mg-Al Layered Double Hydroxides
Authors: Smalenskaite A. , Kaba M. M. , Grigoraviciute-Puroniene I. , Mikoliunaite L. , Zarkov A. , Ramanauskas R. , Morkan I. A. , Kareiva A.
Published: November 2019

In this study, new synthetic approaches for the preparation of thin films of Mg-Al layered double hydroxides (LDHs) have been developed. The LDHs were fabricated by reconstruction of mixed-metal oxides (MMOs) in deionized water. The MMOs were obtained by calcination of the precursor gels. Thin films of sol–gel-derived Mg-Al LDHs were deposited on silicon and stainless-steel substrates using the dip-coating technique by a single dipping process, and the deposited film was dried before the new layer was added. Each layer in the preparation of the Mg-Al LDH multilayers was separately annealed at 70 °C or 300 °C in air. Fabricated Mg-Al LDH coatings were characterized by X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), and atomic force microscopy (AFM). It was discovered that the diffraction lines of Mg3Al LDH thin films are sharper and more intensive in the sample obtained on the silicon substrate, confirming a higher crystallinity of synthesized Mg3Al LDH. However, in both cases the single-phase crystalline Mg-Al LDHs have formed. To enhance the sol–gel processing, the viscosity of the precursor gel was increased by adding polyvinyl alcohol (PVA) solution. The LDH coatings could be used to protect different substrates from corrosion, as catalyst supports, and as drug-delivery systems in medicine.
Influence of Geometric Dimensions on the Performance of Switched Reluctance Machine
Authors: Mamede Ana Camila F. , Camacho José Roberto , Araújo Rui Esteves
Published: November 2019

In a design of a switched reluctance machine, there are a number of parameters that are chosen empirically inside a certain interval, therefore to find an optimum geometry it is necessary to determine how each parameter acts on the performance of the machine. This work presents a study on the influence of geometric dimensions on the performance of the switched reluctance machine. The analysis is done through finite element simulations based on the variation of one parameter while the others are fixed. Graphical and numerical results of torque and magnetic flux are presented for a 6/4 three-phase machine and an 8/6 four-phase machine. The study presented aims to provide consistent data on which dimensions should be modified for specific applications, and thus to base choices made in the design and optimization stage.
Multipolar Fuzzy p-Ideals of BCI-Algebras
Authors: Takallo Mohammad Mohseni , Ahn Sun Shin , Borzooei Rajab Ali , Jun Young Bae
Published: November 2019

The notion of (normal) m-polar ( ∈ , ∈ ) -fuzzy p-ideals of BCI-algebras is introduced, and several properties are investigated. Relations between an m-polar ( ∈ , ∈ ) -fuzzy ideal and an m-polar ( ∈ , ∈ ) -fuzzy p-ideal are displayed, and conditions for an m-polar ( ∈ , ∈ ) -fuzzy ideal to be an m-polar ( ∈ , ∈ ) -fuzzy p-ideal are provided. Characterization of m-polar ( ∈ , ∈ ) -fuzzy p-ideals are considered. Given an m-polar ( ∈ , ∈ ) -fuzzy ideal (resp., m-polar ( ∈ , ∈ ) -fuzzy p-ideal), a normal m-polar ( ∈ , ∈ ) -fuzzy ideal (resp., normal m-polar ( ∈ , ∈ ) -fuzzy p-ideal) is established. Using an m-polar ( ∈ , ∈ ) -fuzzy ideal, the quotient structure of BCI-algebras is constructed.
Prediction of Eddy Current Losses in Cooling Tubes of Direct Cooled Windings in Electric Machines
Authors: Ibrahim Mohamed Nabil Fathy , Sergeant Peter
Published: November 2019

The direct coil cooling method is one of the existing cooling techniques for electric machines with concentrated windings, in which cooling tubes of conductive material are inserted between the windings. In such cases, eddy current losses are induced in those cooling tubes because of the time variant magnetic field. To compute the cooling tubes losses, either a transient finite element simulation (mostly based on commercial software), or a full analytical method, which is more complex to be constructed, is required. Instead, this paper proposes a simple and an accurate combined semi-analytical-finite element method to calculate the losses of electric machines having cooling tubes. The 2D magnetostatic solution of the magnetic field is obtained e.g., using the free package “FEMM”. Then, the eddy current losses in the tubes are computed using simple analytical equations. In addition, the iron core losses could be obtained. In order to validate the proposed method, two cases are investigated. In Case 1, a six-toothed stator of a switched reluctance machine (SRM), without rotor, is employed in which six cooling tubes are used while in Case 2 a complete rotating SRM is studied. The proposed method is validated by a 2D transient simulation in the commercial software “ANSYS Maxwell” and also by experimental measurements. Evidently, the proposed method is simple and fast to be constructed and it is almost free of cost.
Deciphering Molecular Mechanism of the Neuropharmacological Action of Fucosterol through Integrated System Pharmacology and In Silico Analysis
Authors: Hannan Md. Abdul , Dash Raju , Sohag Abdullah Al Mamun , Moon Il Soo
Published: November 2019

Fucosterol is an algae-derived unique phytosterol having several medicinal properties, including antioxidant, anti-inflammatory, anticholinesterase, neuroprotective, and so on. Accumulated evidence suggests a therapeutic promise of fucosterol in neurodegeneration; however, the in-depth pharmacological mechanism of its neuroprotection is poorly understood. Here, we employed system pharmacology and in silico analysis to elucidate the underlying mechanism of neuropharmacological action of fucosterol against neurodegenerative disorders (NDD). Network pharmacology revealed that fucosterol targets signaling molecules, receptors, enzymes, transporters, transcription factors, cytoskeletal, and various other proteins of cellular pathways, including tumor necrosis factor (TNF), mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt), neurotrophin, and toll-like receptor (TLR) signaling, which are intimately associated with neuronal survival, immune response, and inflammation. Moreover, the molecular simulation study further verified that fucosterol exhibited a significant binding affinity to some of the vital targets, including liver X-receptor-beta (LXR-), glucocorticoid receptor (GR), tropomyosin receptor kinase B (TrkB), toll-like receptor 2/4 (TLR2/4), and β -secretase (BACE1), which are the crucial regulators of molecular and cellular processes associated with NDD. Together, the present system pharmacology and in silico findings demonstrate that fucosterol might play a significant role in modulating NDD-pathobiology, supporting its therapeutic application for the prevention and treatment of NDD.
Biomaterials in Gastroenterology: A Critical Overview
Authors: Goldis Adrian , Goldis Ramona , Chirila Traian V.
Published: November 2019

In spite of the large diversity of diagnostic and interventional devices associated with gastrointestinal endoscopic procedures, there is little information on the impact of the biomaterials (metals, polymers) contained in these devices upon body tissues and, indirectly, upon the treatment outcomes. Other biomaterials for gastroenterology, such as adhesives and certain hemostatic agents, have been investigated to a greater extent, but the information is fragmentary. Much of this situation is due to the paucity of details disclosed by the manufacturers of the devices. Moreover, for most of the applications in the gastrointestinal (GI) tract, there are no studies available on the biocompatibility of the device materials when in intimate contact with mucosae and other components of the GI tract. We have summarized the current situation with a focus on aspects of biomaterials and biocompatibility related to the device materials and other agents, with an emphasis on the GI endoscopic procedures. Procedures and devices used for the control of bleeding, for polypectomy, in bariatrics, and for stenting are discussed, particularly dwelling upon the biomaterial-related features of each application. There are indications that research is progressing steadily in this field, and the establishment of the subdiscipline of “gastroenterologic biomaterials” is not merely a remote projection. Upon the completion of this article, the gastroenterologist should be able to understand the nature of biomaterials and to achieve a suitable and beneficial perception of their significance in gastroenterology. Likewise, the biomaterialist should become aware of the specific tasks that the biomaterials must fulfil when placed within the GI tract, and regard such applications as both a challenge and an incentive for progressing the research in this field.
Generation of Pure State Photon Triplets in the C-Band
Authors: Su Xi-Rong , Huang Yi-Wen , Xiang Tong , Li Yuan-Hua , Chen Xian-Feng
Published: November 2019

In this work, the cascaded second-order spontaneous parametric down-conversion (SPDC) is considered to produce pure state photon triplets in periodically poled lithium niobite (PPLN) doped with 5% MgO. A set of parameters are optimized through calculating the Schmidt number of two-photon states generated by each down-conversion process with different pump durations and crystal lengths. We use a Gaussian filter in part and obtain three photons with 100% purity in spectrum. We provide a feasible and unprecedented scheme to manipulate the spectrum purity of photon triplets in the communication band (C-band).
Prevalence, Distribution, and Phylogeny of Type Two Toxin-Antitoxin Genes Possessed by Cronobacter Species where C. sakazakii Homologs Follow Sequence Type Lineages
Authors: Finkelstein Samantha , Negrete Flavia , Jang Hyein , Gangiredla Jayanthi , Mammel Mark , Patel Isha R. , Chase Hannah R. , Woo JungHa , Lee YouYoung , Wang Caroline Z. , Weinstein Leah , Tall Ben D. , Gopinath Gopal R.
Published: November 2019

Cronobacter species are a group of foodborne pathogenic bacteria that cause both intestinal and systemic human disease in individuals of all age groups. Little is known about the mechanisms that Cronobacter employ to survive and persist in foods and other environments. Toxin–antitoxin (TA) genes are thought to play a role in bacterial stress physiology, as well as in the stabilization of horizontally-acquired re-combinatorial elements such as plasmids, phage, and transposons. TA systems have been implicated in the formation of a persistence phenotype in some bacterial species including Escherichia coli and Salmonella. This project’s goal was to understand the phylogenetic relatedness among TA genes present in Cronobacter. Preliminary studies showed that two typical toxin genes, fic and hipA followed species evolutionary lines. A local database of 22 TA homologs was created for Cronobacter sakazakii and a Python version 3 shell script was generated to extract TA FASTA sequences present in 234 C. sakazakii genomes previously sequenced as part of Center for Food Safety and Applied Nutrition’s (CFSAN) GenomeTrakr project. BLAST analysis showed that not every C. sakazakii strain possessed all twenty-two TA loci. Interestingly, some strains contained either a toxin or an antitoxin component, but not both. Five common toxin genes: ESA_00258 (parDE toxin-antitoxin family), ESA_00804 (relBE family), ESA_01887 (relBE family), ESA_03838 (relBE family), and ESA_04273 (YhfG-Fic family) were selected for PCR analysis and the primers were designed to detect these genes. PCR analysis showed that 55 of 63 strains possessed three of these genes Sequence analysis identified homologs of the target genes and some of the strains were PCR-negative for one or more of the genes, pointing to potential nucleotide polymorphisms in those loci or that these toxin genes were absent. Phylogenetic studies using a Cronobacter pan genomic microarray showed that for the most part TAs follow species evolutionary lines except for a few toxin genes possessed by some C. malonaticus and C. universalis strains; this demonstrates that some TA orthologues share a common phylogeny. Within the C. sakazakii strains, the prevalence and distribution of these TA homologs by C. sakazakii strain BAA-894 (a powdered infant formula isolate) followed sequence-type evolutionary lineages. Understanding the phylogeny of TAs among the Cronobacter species is essential to design future studies to realize the physiological mechanisms and roles for TAs in stress adaptation and persistence of Cronobacter within food matrices and food processing environments.
Combined Use of the Ab105-2φΔCI Lytic Mutant Phage and Different Antibiotics in Clinical Isolates of Multi-Resistant Acinetobacter baumannii
Authors: Blasco Lucia , Ambroa Anton , Lopez Maria , Fernandez-Garcia Laura , Bleriot Ines , Trastoy Rocio , Ramos-Vivas Jose , Coenye Tom , Fernandez-Cuenca Felipe , Vila Jordi , Martinez-Martinez Luis , Rodriguez-Baño Jesus , Pascual Alvaro , Cisneros Jose Miguel , Pachon Jeronimo , Bou German , Tomas Maria
Published: November 2019

Phage therapy is an abandoned antimicrobial therapy that has been resumed in recent years. In this study, we mutated a lysogenic phage from Acinetobacter baumannii into a lytic phage (Ab105-2phiΔCI) that displayed antimicrobial activity against A. baumannii clinical strain Ab177_GEIH-2000 (isolated in the GEIH-REIPI Spanish Multicenter A. baumannii Study II 2000/2010, Umbrella Genbank Bioproject PRJNA422585, and for which meropenem and imipenem MICs of respectively, 32 µg/mL, and 16 µg/mL were obtained). We observed an in vitro synergistic antimicrobial effect (reduction of 4 log–7 log CFU/mL) between meropenem and the lytic phage in all combinations analyzed (Ab105-2phiΔCI mutant at 0.1, 1 and 10 MOI and meropenem at 1/4 and 1/8 MIC). Moreover, bacterial growth was reduced by 8 log CFU/mL for the combination of imipenem at 1/4 MIC plus lytic phage (Ab105-2phiΔCI mutant) and by 4 log CFU/mL for the combination of imipenem at 1/8 MIC plus lytic phage (Ab105-2phiΔCI mutant) at both MOI 1 and 10. These results were confirmed in an in vivo model (G. mellonella), and the combination of imipenem and mutant Ab105-2phiΔCI was most effective (p < 0.05). This approach could help to reduce the emergence of phage resistant bacteria and restore sensitivity to antibiotics used to combat multi-resistant strains of Acinetobacter baumannii.
Cell-Wall Hydrolases as Antimicrobials against Staphylococcus Species: Focus on Sle1
Authors: Vermassen Aurore , Talon Régine , Andant Carine , Provot Christian , Desvaux Mickaël , Leroy Sabine
Published: November 2019

Some staphylococcal species are opportunistic pathogens of humans and/or animals with Staphylococcus epidermidis as one of the most important. It causes a broad spectrum of diseases in humans and animals. This species is able to form biofilms and has developed antibiotic resistance, which has motivated research on new antibacterial agents. Cell-wall hydrolases (CWHs) can constitute a potential alternative. Following a hijacking strategy, we inventoried the CWHs of S. epidermidis. The lytic potential of representative CWHs that could be turned against staphylococci was explored by turbidity assays which revealed that cell wall glycosidases were not efficient, while cell wall amidases and cell wall peptidases were able to lyse S. epidermidis. Sle1, which is encoded by chromosomal gene and composed of three anchoring LysM domains and a C-terminal CHAP (cysteine, histidine-dependent amidohydrolase/peptidase) domain, was one of the most active CWHs. The phylogeny of Sle1 revealed seven clusters mostly identified among staphylococci. Sle1 was able to lyse several staphylococcal species, including Staphylococcus aureus, both in planktonic and sessile forms, but not Micrococcus.
[b]-Annulated Halogen-Substituted Indoles as Potential DYRK1A Inhibitors
Authors: Lechner Christian , Flaßhoff Maren , Falke Hannes , Preu Lutz , Loaëc Nadége , Meijer Laurent , Knapp Stefan , Chaikuad Apirat , Kunick Conrad
Published: November 2019

Since hyperactivity of the protein kinase DYRK1A is linked to several neurodegenerative disorders, DYRK1A inhibitors have been suggested as potential therapeutics for Down syndrome and Alzheimer’s disease. Most published inhibitors to date suffer from low selectivity against related kinases or from unfavorable physicochemical properties. In order to identify DYRK1A inhibitors with improved properties, a series of new chemicals based on [b]-annulated halogenated indoles were designed, synthesized, and evaluated for biological activity. Analysis of crystal structures revealed a typical type-I binding mode of the new inhibitor 4-chlorocyclohepta[b]indol-10(5H)-one in DYRK1A, exploiting mainly shape complementarity for tight binding. Conversion of the DYRK1A inhibitor 8-chloro-1,2,3,9-tetrahydro-4H-carbazol-4-one into a corresponding Mannich base hydrochloride improved the aqueous solubility but abrogated kinase inhibitory activity.
Rapid Analysis of the Chemical Compositions in Semiliquidambar cathayensis Roots by Ultra High-Performance Liquid Chromatography and Quadrupole Time-of-Flight Tandem Mass Spectrometry
Authors: Yang Li , Liu Rong-Hua , He Jun-Wei
Published: November 2019

Semiliquidambar cathayensis Chang was a traditional medicinal plant and used to treat rheumatism arthritis and rheumatic arthritis for centuries in China with no scientific validation, while only 15 components were reported. Thus, a rapid, efficient, and precise method based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was applied in both positive- and negative-ion modes to rapidly analysis the main chemical compositions in S. cathayensis for the first time. Finally, a total of 85 chemical compositions, including 35 alkaloids, 12 flavonoids, 7 terpenoids, 5 phenylpropanoids, 9 fatty acids, 7 cyclic peptides, and 10 others were identified or tentatively characterized in the roots of S. cathayensis based on the accurate mass within 5 ppm error. Moreover, alkaloid, flavonoid, phenylpropanoid, and cyclic peptide were reported from S. cathayensis for the first time. This rapid and sensitive method was highly useful to comprehend the chemical compositions and will provide scientific basis for further study on the material basis, mechanism and clinical application of S. cathayensis roots.
Black Ginseng and Ginsenoside Rb1 Promote Browning by Inducing UCP1 Expression in 3T3-L1 and Primary White Adipocytes
Authors: Park Seon-Joo , Park Miey , Sharma Anshul , Kim Kihyun , Lee Hae-Jeung
Published: November 2019

In this study, we investigated the effects of black ginseng (BG) and ginsenoside Rb1, which induced browning effects in 3T3-L1 and primary white adipocytes (PWATs) isolated from C57BL/6 mice. BG and Rb1 suppressed the expressions of CCAAT/enhancer-binding protein alpha (C/EBPα) and sterol regulatory element-binding transcription factor-1c (SREBP-1c), whereas the expression level of peroxisome proliferator-activated receptor gamma (PPARγ) was increased. Furthermore, BG and Rb1 enhanced the protein expressions of the brown-adipocyte-specific markers PR domain containing 16 (PRDM16), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α), and uncoupling protein 1 (UCP1). These results were further supported by immunofluorescence images of mitochondrial biogenesis. In addition, BG and Rb1 induced expressions of brown-adipocyte-specific marker proteins by AMP-activated protein kinase (AMPK) activation. BG and Rb1 exert antiobesity effects by inducing browning in 3T3-L1 cells and PWATs through AMPK-mediated pathway activation. We suggest that BG and Rb1 act as potential functional antiobesity food agents.
Low Carbohydrate and Low-Fat Diets: What We Don’t Know and Why We Should Know It
Authors: Seid Heather , Rosenbaum Michael
Published: November 2019

In the 1940s, the diet-heart hypothesis proposed that high dietary saturated fat and cholesterol intake promoted coronary heart disease in “at-risk” individuals. This hypothesis prompted federal recommendations for a low-fat diet for “high risk” patients and as a preventive health measure for everyone except infants. The low carbohydrate diet, first used to treat type 1 diabetes, became a popular obesity therapy with the Atkins diet in the 1970s. Its predicted effectiveness was based largely on the hypothesis that insulin is the causa prima of weight gain and regain via hyperphagia and hypometabolism during and after weight reduction, and therefore reduced carbohydrate intake would promote and sustain weight loss. Based on literature reviews, there are insufficient randomized controlled inpatient studies examining the physiological significance of the mechanisms proposed to support one over the other. Outpatient studies can be confounded by poor diet compliance such that the quality and quantity of the energy intake cannot be ascertained. Many studies also fail to separate macronutrient quantity from quality. Overall, there is no conclusive evidence that the degree of weight loss or the duration of reduced weight maintenance are significantly affected by dietary macronutrient quantity beyond effects attributable to caloric intake. Further work is needed.
Dietary Patterns and the Double Burden of Malnutrition in Mexican Adolescents: Results from ENSANUT-2006
Authors: Zárate-Ortiz Arli Guadalupe , Melse-Boonstra Alida , Rodríguez-Ramírez Sonia , Hernández-Cordero Sonia , Feskens Edith J. M.
Published: November 2019

Mexico is facing the double burden of malnutrition, and adolescents are not an exception. Diet plays an important role, both in causing overweight and undernutrition. This study aimed to describe the dietary patterns (DPs) of Mexican adolescents and to examine its association with nutritional status using data from adolescents aged 12–19 years (n = 7380) from the National Survey of Health and Nutrition (ENSANUT-2006). Principal component analysis was used to derivate the DPs. Associations between DP and nutritional status were determined by prevalence ratio (PR). Four DPs were identified: nontraditional and breakfast-type, Western, plant-based, and protein-rich. The prevalence of overweight and obesity was higher in adolescents who scored high on the Western pattern (PR: 1.15, 95% CI: 1.08–1.21) or on the plant-based pattern (PR: 1.09, 95% CI: 1.03–1.17). The Western pattern was positively associated with anemia in girls (PR: 1.18, 95% CI: 1.03–1.35), while the nontraditional and breakfast-type pattern was inversely associated with anemia in adolescents aged 12–15 years (PR: 0.87, 95% CI: 0.76–0.99) and in girls (PR: 0.84, 95% CI: 0.75–0.97). The Western and plant-based patterns were simultaneously associated with overweight–obesity and at least one indicator of undernutrition. In the context of the double burden of malnutrition, dietary advice must consider malnutrition in all its forms.
Chemical Probes for the Adenosine Receptors
Authors: Federico Stephanie , Lassiani Lucia , Spalluto Giampiero
Published: November 2019

Research on the adenosine receptors has been supported by the continuous discovery of new chemical probes characterized by more and more affinity and selectivity for the single adenosine receptor subtypes (A1, A2A, A2B and A3 adenosine receptors). Furthermore, the development of new techniques for the detection of G protein-coupled receptors (GPCR) requires new specific probes. In fact, if in the past radioligands were the most important GPCR probes for detection, compound screening and diagnostic purposes, nowadays, increasing importance is given to fluorescent and covalent ligands. In fact, advances in techniques such as fluorescence resonance energy transfer (FRET) and fluorescent polarization, as well as new applications in flow cytometry and different fluorescence-based microscopic techniques, are at the origin of the extensive research of new fluorescent ligands for these receptors. The resurgence of covalent ligands is due in part to a change in the common thinking in the medicinal chemistry community that a covalent drug is necessarily more toxic than a reversible one, and in part to the useful application of covalent ligands in GPCR structural biology. In this review, an updated collection of available chemical probes targeting adenosine receptors is reported.
Synthesis, Principles, and Properties of Magnetite Nanoparticles for In Vivo Imaging Applications—A Review
Authors: Wallyn Justine , Anton Nicolas , Vandamme Thierry F.
Published: November 2019

The current nanotechnology era is marked by the emergence of various magnetic inorganic nanometer-sized colloidal particles. These have been extensively applied and hold an immense potential in biomedical applications including, for example, cancer therapy, drug nanocarriers (NCs), or in targeted delivery systems and diagnosis involving two guided-nanoparticles (NPs) as nanoprobes and contrast agents. Considerable efforts have been devoted to designing iron oxide NPs (IONPs) due to their superparamagnetic (SPM) behavior (SPM IONPs or SPIONs) and their large surface-to-volume area allowing more biocompatibility, stealth, and easy bonding to natural biomolecules thanks to grafted ligands, selective-site moieties, and/or organic and inorganic corona shells. Such nanomagnets with adjustable architecture have been the topic of significant progresses since modular designs enable SPIONs to carry out several functions simultaneously such as local drug delivery with real-time monitoring and imaging of the targeted area. Syntheses of SPIONs and adjustments of their physical and chemical properties have been achieved and paved novel routes for a safe use of those tailored magnetic ferrous nanomaterials. Herein we will emphasis a basic notion about NPs magnetism in order to have a better understanding of SPION assets for biomedical applications, then we mainly focus on magnetite iron oxide owing to its outstanding magnetic properties. The general methods of preparation and typical characteristics of magnetite are reviewed, as well as the major biomedical applications of magnetite.
Bone Morphogenic Protein 2-Loaded Porous Silicon Carriers for Osteoinductive Implants
Authors: Rosenberg Michal , Shilo Dekel , Galperin Leonid , Capucha Tal , Tarabieh Karim , Rachmiel Adi , Segal Ester
Published: November 2019

Bone morphogenetic proteins (BMPs) are probably the most important growth factors in bone formation and healing. However, the utilization of BMPs in clinical applications is mainly limited due to the protein poor solubility at physiological pH, rapid clearance and relatively short biological half-life. Herein, we develop degradable porous silicon (PSi)-based carriers for sustained delivery of BMP-2. Two different loading approaches are examined, physical adsorption and covalent conjugation, and their effect on the protein loading and release rate is thoroughly studied. The entrapment of the protein within the PSi nanostructures preserved its bioactivity for inducing osteogenic differentiation of rabbit bone marrow mesenchymal stems cells (BM-MSCs). BM-MSCs cultured with the BMP-2 loaded PSi carriers exhibit a relatively high alkaline phosphatase (ALP) activity. We also demonstrate that exposure of MSCs to empty PSi (no protein) carriers generates some extent of differentiation due to the ability of the carrier’s degradation products to induce osteoblast differentiation. Finally, we demonstrate the integration of these promising BMP-2 carriers within a 3D-printed patient-specific implant, constructed of poly(caprolactone) (PCL), as a potential bone graft for critical size bone defects.
Design and Evaluation of pH-Dependent Nanosystems Based on Cellulose Acetate Phthalate, Nanoparticles Loaded with Chlorhexidine for Periodontal Treatment
Authors: Vidal-Romero Gustavo , Zambrano-Zaragoza María L. , Martínez-Acevedo Lizbeth , Leyva-Gómez Gerardo , Mendoza-Elvira Susana E. , Quintanar-Guerrero David
Published: November 2019

This work aimed to develop and evaluate pH-dependent systems based on nanospheres (NSphs) and nanocapsules (NCs) loaded with chlorhexidine (CHX) base as a novel formulation for the treatment of periodontal disease. Cellulose acetate phthalate (CAP) was employed as a pH-dependent polymeric material. The NSphs and NCs were prepared using the emulsion-diffusion technique and then characterized according to encapsulation efficiency (EE), size, zeta-potential, morphology, thermal properties, release profiles and a preliminary clinical panel test. The formulations showed 77% and 61% EE and 57% and 84% process efficiency (PE), respectively. Both systems were spherical with an average size of 250–300 nm. Differential scanning calorimetry (DSC) studies showed that the drug has the potential to be dispersed molecularly in the NSph matrix or dissolved in the oily center of the NCs. The CHX release test revealed that the release of NSphs-CHX follows Fickian diffusion involving diffusion-erosion processes. The NCs showed a slower release than the NSphs, following non-Fickian diffusion, which is indicative of anomalous transport. These nanosystems may, therefore, be employed as novel formulations for treating periodontal disease, due to (1) their coverage of a large surface area, (2) the controlled release of active substances at different pH, and (3) potential gingival tissue infiltration.
Enhancement in Site-Specific Delivery of Carvacrol against Methicillin Resistant Staphylococcus aureus Induced Skin Infections Using Enzyme Responsive Nanoparticles: A Proof of Concept Study
Authors: Mir Maria , Ahmed Naveed , Permana Andi Dian , Rodgers Aoife Maria , Donnelly Ryan F. , Rehman Asim.ur.
Published: November 2019

Methicillin resistant Staphylococcus aureus (MRSA) induced skin infections have become a challenging problem due to the escalating antibiotic resistance. Carvacrol (CAR) has been reported to be effective against MRSA. However, due to its characteristics, CAR exhibits low skin retention. In this study, CAR was formulated into site-specific nanoparticle (NPs) delivery system using poly(ε-caprolactone) (PCL), following incorporation into a hydrogel matrix to facilitate dermal delivery. The release study exhibited significantly higher release of CAR from PCL NPs in the presence of bacterial lipase, highlighting its potential for differential delivery. Moreover, encapsulation of CAR in PCL NPs resulted in a two-fold increase in its anti-MRSA activity. Dermatokinetic studies revealed that the NPs loaded hydrogel was able to enhance skin retention of CAR after 24 h (83.29 ± 3.15%), compared to free CAR-loaded hydrogel (0.85 ± 0.14%). Importantly, this novel approach exhibited effective antimicrobial activity in an ex-vivo skin infection model. Hence, these findings have proven the concept that the loading of CAR into a responsive NPs system can lead to sustained antimicrobial effect at the desired site, and may provide a novel effective approach for treatment of MRSA induced skin infections. However, further studies must be conducted to investigate in-vivo efficacy of the developed system in an appropriate infection model.
Tunable THz Graphene Filter Based on Cross-In-Square-Shaped Resonators Metasurface
Authors: Zaitsev Anton , Grebenchukov Alexander , Khodzitsky Mikhail
Published: November 2019

The tunable terahertz (THz) Fano-resonant filter based on hybrid metal-graphene metamaterial was proposed. The optical parameters of metasurface with unit cell in the form of a cross-shaped graphene sheet in the center of a square gold ring were simulated by the finite element method using a surface conductivity model of a graphene monolayer. The narrowband modulation of the transmission by varying the Fermi level of the graphene and the position of graphene cross inside the metal ring was demonstrated. Simulation results were well explained theoretically using a three-coupled oscillator model. The proposed device can be used as a narrowband filter in wireless THz communication systems and sensing applications.
Phenolic Composition and Antimicrobial Activity of Different Emirati Date (Phoenix dactylifera L.) Pits: A Comparative Study
Authors: Hussain M. Iftikhar , Semreen Mohammad H. , Shanableh Abdallah , Khattak Muhammad Nasir Khan , Saadoun Ismail , Ahmady Islam M. , Mousa Muath , Darwish Nora , Radeef Wameed , Soliman Sameh S. M.
Published: November 2019

The biochemical composition, secondary metabolites (phenolic compounds, flavonoids) and antimicrobial potential of different varieties of Emirati date (Phoenix dactylifera L.) pits were investigated. Total phenolic acids (TPC) and total flavonoid contents (TFC) of the different date pits were measured using a Folin–Ciocalteau reagent. Different organic solvents [(n-hexane; H2O: EtOH (1:1); ethyl acetate; acetone: Water (1:1); and methanol: Chloroform (1:1)] were compared to evaluate the phytotoxicity of Ajwa, Fard, Khalas, Khodari, Abu Maan, Lulu, and Mabroom date pits. The antimicrobial activity of the date pit extracts were evaluated by means of agar-well diffusion assay on Staphylococcus aureus (ATCC 29123), Escherichia coli (ATCC 25922) and Candida albicans (ATCC 66027). Minimum inhibitory concentrations (MICs) were measured following clinical laboratory standardization institute (CLSI) protocol. The biochemical analyses of date pits indicate that TPC were ranged from 7.80 mg of equivalent gallic acid⁄100 g dry weight in Ajwa to 4.65 mg in Mabroom. The TFC were ranged between 1.6–4.54 mg of equivalent catechin⁄100 g dry weight. Ajwa pit extract showed good quality traits (higher protein, lower ash content, and intermediate dietary fiber). The results indicate that the ethyl acetate extract of Khalas and Khodari inhibited S. aureus with an inhibition zone diameter of 20 mm and MIC of 10 mg/mL. Abu Mann pit extract inhibited the S. aureus and also decreased the population of E. coli. The diameter of inhibition zone was 15, 16, and 18 mm after treatment with Ajwa extracts, while the MICs were 7.5 and 5 mg/mL. The MeOH: CFM extract of Abu Mann and Ajwa was more potent against E. coli bacteria than any other extract. This work demonstrates that the Emirati date pits extract has antimicrobial (antibacterial, antifungal) potential and can be used as phytotoxic natural compounds.
Electrical Current Map and Bulk Conductivity of Carbon Fiber-Reinforced Nanocomposites
Authors: Guadagno Liberata , Vertuccio Luigi , Naddeo Carlo , Raimondo Marialuigia , Barra Giuseppina , De Nicola Felice , Volponi Ruggero , Lamberti Patrizia , Spinelli Giovanni , Tucci Vincenzo
Published: November 2019

A suitably modified resin film infusion (RFI) process was used for manufacturing carbon fiber-reinforced composites (CFRCs) impregnated with a resin containing nanocages of glycidyl polyhedral oligomeric silsesquioxane (GPOSS) for enhancing flame resistance and multi-wall carbon nanotubes (MWCNTs) to contrast the electrical insulating properties of the epoxy resin. The effects of the different numbers (7, 14 and 24) of the plies on the equivalent direct current (DC) and alternating current (AC) electrical conductivity were evaluated. All the manufactured panels manifest very high values in electrical conductivity. Besides, for the first time, CFRC strings were analyzed by tunneling atomic force microscopy (TUNA) technique. The electrical current maps highlight electrically conductive three-dimensional networks incorporated in the resin through the plies of the panels. The highest equivalent bulk conductivity is shown by the seven-ply panel characterized by the parallel (σ//0°) in-plane conductivity of 16.19 kS/m. Electrical tests also evidence that the presence of GPOSS preserves the AC electrical stability of the panels.
Polymer Stabilized Liquid Crystal Smart Window with Flexible Substrates Based on Low-Temperature Treatment of Polyamide Acid Technology
Authors: Zhang Yang , Wang Changrui , Zhao Wei , Li Ming , Wang Xiao , Yang Xiulan , Hu Xiaowen , Yuan Dong , Yang Weiping , Zhang Yi , Lv Pengrong , He Jialin , Zhou Guofu
Published: November 2019

Polymer stabilized liquid crystal (PSLC) devices can be used as smart privacy windows that switch between transparent and opaque states. The polyimide alignment layer of a PSLC device is usually obtained by the treatment of polyamide acid (PAA) with temperatures over 200 °C. This hinders the fabrication of PSLC devices on flexible substrates, which melt at these high temperatures. In this work, the fabrication of a PSLC alignment layer using a lower temperature that is compatible with most flexible substrates, is demonstrated. It was found that the treatment of PAA at 150 °C could generate the same alignment for liquid crystals. Based on this, a PSLC device was successfully fabricated on a flexible polyethylene terephthalate (PET) substrate, demonstrating excellent electro-optic performances.
Evaluation of Municipal Solid Wastes Based Energy Potential in Urban Pakistan
Authors: Siddiqi Muhammad Mobin , Naseer Muhammad Nihal , Abdul Wahab Yasmin , Hamizi Nor Aliya , Badruddin Irfan Anjum , Chowdhury Zaira Zaman , Akbarzadeh Omid , Johan Mohd Rafie , Khan T. M. Yunus , Kamangar Sarfaraz
Published: November 2019

Solid waste management needs re-evaluating in developing countries like Pakistan, which currently employs landfilling as a first option. Over time, increasing population will result in decreasing space for landfill sites, ultimately increasing the cost of landfilling, while increasing accumulated waste will cause pollution. Locating and preparing a sanitary landfill includes the securing of large sectors and also everyday activity with the end goal to limit potential negative impacts. Energy production from municipal solid waste (MSW) is a perceptive idea for large cities, such as Karachi, as waste, which is an undesirable output that adds to land and air pollution, is transformed into a vital source of energy. The current study strives to provide a destination to solid waste by evaluating the energy potential that waste provides for power generation by the process of incineration. A sustainable energy generation plant based on the Rankine cycle is proposed. This study evaluates the various landfill sites in the case study area to determine their sustainability for a waste to energy (WtE) plant. The implementation of the proposed plant will not only provide an ultimate destination to waste but also generate 121.9 MW electricity at 25% plant efficiency. Thus, the generated electricity can be used to run a WtE plant and meet the energy requirements of the residents.