bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Periodontal Treatment on the Reactive Hyperemia Index. A 1-Year Follow-Up Pilot Study
Authors: Donders H. C. M. , Veth E. O. , Edens M. A. , van ’t Hof A. W. J. , de Lange J. , Loos B. G.
Published: April 2022

Background

Periodontitis is a chronic multifactorial inflammatory disease of the supportive tissues of the teeth. In more recent years, remarkable epidemiological and pathophysiological associations between periodontitis and cardiovascular disease (CVD) have been presented. Whether or not treatment of periodontitis is valuable for primary or secondary prevention of cardiovascular disease, has not yet been fully established. In this practice-based pilot study we focused on primary prevention of cardiovascular disease, by investigating the effect of periodontal treatment on the earliest detectable stage of CVD; endothelial dysfunction.

Methods

Otherwise healthy periodontitis and non-periodontitis participants 45–70 years of age were included in the study. One year after completing periodontal (non-surgical and surgical) treatment of the periodontitis patients and 1 year after inclusion of the controls, all baseline measurements were repeated. Full-mouth examinations were performed by a periodontist to determine their Periodontal Inflamed Surface Area (PISA) score and other dental parameters. To assess the cardiovascular conditions, endothelial function through the reactive hyperemia index (RHI) assessed by the EndoPAT™, and several physical and biochemical parameters were measured.

Results

21 patients with diagnosed, untreated periodontitis and 21 participants without periodontitis were included in this follow-up study. After periodontal therapy in the periodontitis patients, the PISA reduced significantly. The RHI did not show a significant improvement after treatment of the periodontitis patients (−0.1 ± 0.8, p = 0.524). Similarly, other secondary cardiovascular outcome measurements, hsCRP, total cholesterol, HDL cholesterol, triglycerides, HbA1c, and systolic blood pressure did not improve significantly after periodontal treatment. Controls did not show any significant changes in the RHI, in other CVD parameters and in the PISA after 1-year follow-up.

Conclusion

In this practice-based pilot study, periodontal treatment did not improve the endothelial function in otherwise healthy adults with periodontitis. Future studies are needed to be of larger size and could focus on periodontitis patients with co-morbidities to investigate whether periodontal treatment has secondary preventive effect on endothelial function and other CVD parameters.

Clinical Trial Registration

[www.ClinicalTrials.gov], identifier [ISRCTN55656827].
Polypills for Primary Prevention of Cardiovascular Disease: A Systematic Review and Meta-Analysis
Authors: Kandil Omneya A. , Motawea Karam R. , Aboelenein Merna M. , Shah Jaffer
Published: April 2022

Purpose

To evaluate the effect of polypills on the primary prevention of cardiovascular (CV) events using data from clinical trials.

Methods

We searched PubMed, Web of Science, EBSCO, and SCOPUS throughout May 2021. Two authors independently screened articles for the fulfillment of inclusion criteria. The RevMan software (version 5.4) was used to calculate the pooled risk ratios (RRs) and mean differences (MDs), along with their associated confidence intervals (95% CI).

Results

Eight trials with a total of 20653 patients were included. There was a significant reduction in the total number of fatal and non-fatal CV events among the polypill group [RR (95% CI) = 0.71 (0.63, 0.80); P-value < 0.001]. This reduction was observed in both the intermediate-risk [RR (95% CI) = 0.76 (0.65, 0.89); P-value < 0.001] and high-risk [RR (95% CI) = 0.63 (0.52, 0.76); P-value < 0.001] groups of patients. Subgroup analysis was performed based on the follow-up duration of each study, and benefits were only evident in the five-year follow-up duration group [RR (95% CI) = 0.70 (0.62, 0.79); P-value < 0.001]. Benefits were absent in the one-year-or-less interval group [RR (95% CI) = 0.77 (0.47, 1.29); P-value = 0.330]. Additionally, there was a significant reduction in the 10-year predicted cardiovascular risk in the polypill group [MD (95% CI) = −3.74 (−5.96, −1.51); P-value < 0.001], as compared to controls.

Conclusion

A polypill regimen decreases the incidence of fatal and non-fatal CV events in patients with intermediate- and high- cardiovascular risk, and therefore may be an effective treatment for these patients.
Gaussian Beam Migration for Free-Surface Multiples in VSP
Authors: Yu Dai , Yang Feilong , Wen Bo , Wang Ying , Huang Dezhi , Zhao Chi
Published: April 2022

The vertical seismic profiling (VSP) Gaussian beam migration is a seismic imaging method with both computational efficiency and imaging precision. It can get high-resolution structural features near the well. However, the VSP migration based on primary reflection imaging is limited by the observation system, which makes it unable to image for the shallow structure at far offset. The free-surface multiples in VSP can expand the illumination range for far offset effectively. Therefore, Gaussian beam migration (GBM) for free-surface multiples imaging in VSP is proposed. Firstly, based on the ground Gaussian beam pre-stack depth migration, the method for decomposing the plane wave is improved and adapted into VSP geometry. We decompose the seismic records received by the well into plane waves in different directions according to different window centers and image the plane wave of each direction independently. Secondly, through the mirror image of both the velocity model and VSP receivers, the VSP free-surface multiples are converted into VSP primary reflections which are involved in the VSP Gaussian beam migration method, and thus, the method of the primaries is used to image the free-surface multiples. Finally, the effectiveness and robustness of the proposed method are verified by the theoretical model and practical data.
Characteristics of Lithofacies Combinations and Reservoir Property of Carbonate-Rich Shale in Dongying Depression, Eastern China
Authors: Liu Huimin , Zhang Shun , Liu Yali , Zhang Pengfei , Wei Xiaoliang , Wang Yong , Zhu Deyan , Hu Qinhong , Yang Wanqin , Tang Dong , Ning Fangxing , Guan Li , Bao Youshu
Published: April 2022

Drilling has demonstrated that oil can be produced from the lacustrine shale in the upper part of the fourth member of the Shahejie Formation (Es4s) in Dongying Depression. These wells are often drilled in multiple lithofacies combinations, rather than a single lithofacies. Thus, it is necessary to classify the shale lithofacies combinations to optimize favorable exploration targets. The basic characteristics of carbonate mineral-rich shales in the Es4s were determined by core description, optical microscopy of thin sections, mineral analysis by X-ray diffraction, and scanning electron microscope analysis of argon ion-polished samples, combined with organic geochemical analysis and reservoir property analysis. The depositional setting was determined, and the lithofacies combinations of the shale were divided based on the depositional setting and vertical changes in lithofacies combination. Lithofacies combinations include 1) laminated argillaceous limestone interbedded with the calcareous mudstone (LLM); 2) laminated argillaceous limestone interbedded with dolomite (LLD); and 3) calcareous mudstone intercalated with argillaceous limestone (BML). LLM formed in a semi-humid climate, with limited detrital provenance, while LLD was mainly the product of an arid climate, little detrital input, semi-deep water, and a strongly reducing saltwater environment. In addition to the basal strata in the center of the depression, the LLD lithofacies combination was also widely developed on the gentle slope and the slope area transitional to deep depression in the south of the basin. BML was mainly formed in a semi-humid climate, brackish, deep water, and a reducing environment with many detrital provenances. The porosity in LLM and LLD consists of various types. Intercrystalline pores in clay minerals, intercrystalline pores and dissolution pores in carbonate minerals, interbedded fractures, and ultra-pressure fractures are well developed. The pore width distribution range of both LLM and LLD is large. The porosity of LLM is slightly higher than that of LLD. Interlayers with higher dolomite content have higher permeability. The types of pores in BML with no significant laminae characteristics are less abundant, and it is difficult to form an effective reservoir porosity network from the pores and microfractures, and hence, the reservoir physical properties are relatively poor. Oil saturation index indicates that both LLD and LLM of medium–low degree of evolution have good oil content.
Insulin Resistance is a Risk Factor for Early Miscarriage and Macrosomia in Patients With Polycystic Ovary Syndrome From the First Embryo Transfer Cycle: A Retrospective Cohort Study
Authors: Chen Yuanhui , Guo Jiayu , Zhang Qingwen , Zhang Cuilian
Published: April 2022

Objective

The objective of the study was to explore the effect of insulin resistance on pregnancy outcomes in patients with polycystic ovary syndrome (PCOS) from the first embryo transfer cycle.

Design

This was a single-center, retrospective, observational cohort study.

Patients

Included in the study were women with PCOS for the first embryo transfer.

Main Outcome Measures

Early miscarriage rate and macrosomia rate were the main outcome measures.

Results

With increased HOMA-IR, the early miscarriage rate (7.14, 13.21, and 16.22%, respectively; P = 0.039), macrosomia rate (5.78, 11.79, and 17.58%, respectively; P = 0.026) and the incidence of gestational diabetes (GDM) (10.00, 14.50, and 25.67% respectively; P = 0.002) significantly increased, while the live birth rate markedly decreased (63.03, 55.27, and 47.88%, respectively; P = 0.004). No significant difference was found in clinical pregnancy rate, late miscarriage rate, low birthweight rate and baby gender ratio (all P >0.05). After adjusting for confounding factors, HOMA-IR was an independent risk factor of early miscarriage rate and macrosomia rate.

Conclusion

Insulin resistance is an independent risk factor for early miscarriage and macrosomia in PCOS patients during the first embryo transfer cycle. It is essential to give more attention before and after pregnancy for PCOS women with high HOMA-IR.
Circulating microRNAs Are Associated With Metabolic Markers in Adolescents With Hepatosteatosis
Authors: Lin Haixia , Mercer Kelly E. , Ou Xiawei , Mansfield Kori , Buchmann Robert , Børsheim Elisabet , Tas Emir
Published: April 2022

Background

Altered hepatic microRNA (miRNA) expression may play a role in the development of insulin resistance (IR) and non-alcoholic fatty liver disease (NAFLD). Circulating miRNAs could mirror the liver metabolism.

Objective

This study aimed to assess the relationship between serum miRNA profile in children with obesity, IR, and NAFLD.

Methods

Adolescents with obesity (n = 31) were stratified based on insulin resistance and NAFLD status. One-hundred seventy-nine miRNAs were determined in the serum by quantitative RT-PCR. Differentially expressed miRNAs were compared between groups, and log-transformed levels correlated with metabolic markers and intrahepatic triglyceride.

Results

Serum miR-21-5p, -22-3p, -150-5p, and -155-5p levels were higher in children with IR and NAFLD, and their expression levels correlated with hepatic fat and serum triglyceride. In patients with NAFLD, miR-155-5p correlated with ALT (r = 0.68, p<0.01) and AST (r = 0.64, p<0.01) and miR-21-5p and -22-3p levels correlated with plasma adiponectin (r = -0.71 and r = -0.75, respectively, p<0.05) and fibroblast growth factor-21 (r = -0.73 and r = -0.89, respectively, p<0.01). miR-27-3a level was higher in children without IR and NAFLD.

Conclusions

Several miRNAs are differentially expressed in children with IR and NAFLD. Determining their mechanistic roles may provide newer diagnostic tools and therapeutic targets for pediatric NAFLD.
Molecular Regulators of Muscle Mass and Mitochondrial Remodeling Are Not Influenced by Testosterone Administration in Young Women
Authors: Horwath Oscar , Moberg Marcus , Hirschberg Angelica Lindén , Ekblom Björn , Apró William
Published: April 2022

Testosterone (T) administration has previously been shown to improve muscle size and oxidative capacity. However, the molecular mechanisms underlying these adaptations in human skeletal muscle remain to be determined. Here, we examined the effect of moderate-dose T administration on molecular regulators of muscle protein turnover and mitochondrial remodeling in muscle samples collected from young women. Forty-eight healthy, physically active, young women (28 ± 4 years) were assigned in a random double-blind fashion to receive either T (10 mg/day) or placebo for 10-weeks. Muscle biopsies collected before and after the intervention period were divided into sub-cellular fractions and total protein levels of molecular regulators of muscle protein turnover and mitochondrial remodeling were analyzed using Western blotting. T administration had no effect on androgen receptor or 5α-reductase levels, nor on proteins involved in the mTORC1-signaling pathway (mTOR, S6K1, eEF2 and RPS6). Neither did it affect the abundance of proteins associated with proteasomal protein degradation (MAFbx, MuRF-1 and UBR5) and autophagy-lysosomal degradation (AMPK, ULK1 and p62). T administration also had no effect on proteins in the mitochondria enriched fraction regulating mitophagy (Beclin, BNIP3, LC3B-I, LC3B-II and LC3B-II/I ratio) and morphology (Mitofilin), and it did not alter the expression of mitochondrial fission- (FIS1 and DRP1) or fusion factors (OPA1 and MFN2). In summary, these data indicate that improvements in muscle size and oxidative capacity in young women in response to moderate-dose T administration cannot be explained by alterations in total expression of molecular factors known to regulate muscle protein turnover or mitochondrial remodeling.
Controller Design for Distributed Secondary Voltage Restoration in the Islanded Microgrid
Authors: Feng Yiwei , Ma Jing
Published: April 2022

The extensive usage of the cyber communication system in multiple distributed secondary controls of islanded microgrids (MGs) suggests that the effects of the communication network and security concerns on system stability cannot be negligible. This study proposes a distributed fault-tolerant secondary voltage recovery method for multiple distributed generators (DGs) in the MGs of islanded mode based on a sliding mode surface. Compared to the traditional asymptotically convergent distributed control method, the proposed controller quickly eliminates the voltage deviation caused by the primary control and provides power sharing in the presence of external disturbance and actuator fault, which can regulate the average voltage magnitude of all the DGs to an expected value. The effectiveness of the proposed strategy is verified by the simulation studies conducted in the MATLAB/Simulink platform.
Continuous Cover Forestry and Cost of Carbon Abatement on Mineral Soils and Peatlands
Authors: Ahtikoski Anssi , Rämö Janne , Juutinen Artti , Shanin Vladimir , Mäkipää Raisa
Published: April 2022

Continuous cover forestry (CCF) has proven to financially outperform rotation forestry (RF) with low or even moderate social price of carbon in mineral soils. However, to date there are no studies to compare financial performance of joint production (timber and carbon sequestration) between mineral soils and peatlands when CCF is applied. A vast variety of harvest intervals and intensity (expressed as post-harvest basal area) for a mature spruce-dominated [Picea abies (L.) Karst.] stand on both mineral and peat soils was simulated with process-based ecosystem model, EFIMOD. In addition, four levels of carbon price (0, 25, 50 and 75€/tCO2) were applied in assessing the profitability of joint production (timber and carbon sequestration) associated with CCF. Mineral soil turned out to be superior to peatland in cost-efficiency of carbon sequestration. For instance, the cost of additional ton of CO2 was only €2/tCO2 with a carbon price of €25/tCO2 for a private forest owner (through carbon trading), while on peatland it fluctuated between €30 and €39.5/tCO2, depending on the carbon price applied for a private forest owner (€25-€75/tCO2). In general, mineral soil was more sensitive to harvest interval and intensity than peatland, with respect to cost-efficiency in climate change mitigation.
Study on the Relationship of Root Morphology and Phosphorus Absorption Efficiency With Phosphorus Uptake Capacity in 235 Peanut (Arachis hypogaea L.) Germplasms
Authors: Zhu Suqing , Luo Lu , Zhang Xiurong , Zhao Meiyu , Wang Xiaoqian , Zhang Junjie , Wan Qian , Li Xianrong , Wan Yongshan , Zhang Kun , Liu Fengzhen
Published: April 2022

Peanut (Arachis hypogaea L.) is a significant oil and protein crop. Its yields greatly depend on the availability of phosphorus (P). Root morphology and P absorption efficiency are important factors affecting the P uptake capacity, but their relationships in peanuts are rarely reported. Here, we report the effect of root morphology and P absorption efficiency on the P uptake capacity in peanuts using 235 germplasms. In this work, we use the P uptake rate per plant to reflect the P uptake capacity. The P uptake capacity was significantly increased after low-P treatment and showed great differences among the germplasms. The germplasms with higher P absorption efficiency and a well-developed root system have higher P uptake capacity. Under both P conditions, the P absorption efficiency plays more important roles than root morphology in P uptake capacity, and the P uptake rate per unit root dry weight and the P uptake rate per unit root surface area contributed the most. Root morphology contributes more to the P uptake capacity under low-P treatment than under sufficient-P conditions, and root surface area contributed the most. Forty-eight germplasms with higher P uptake capacity were screened, and they had three different uptake strategies under low-P treatment. These findings indicated that low-P stress induces root growth and improves the P absorption efficiency of peanuts to ensure the plant gets enough P; provides new insights into the relationship between the P uptake capacity, P absorption efficiency, and root morphology; and furnishes important evaluation indexes for high P-efficient germplasm selection.
Decision of Comprehensive Development Level of Reclaimed Water Based on Improved Catastrophe Theory: A Case Study in Yunnan Province, China
Authors: Huang Xin , Qiu Lin
Published: April 2022

In order to utilize reclaimed water resources reasonably and efficiently, it is necessary to strengthen the evaluation of reclaimed water. However, a major problem with traditional reclaimed water assessment methods is that it mostly focuses on a single aspect. In this study, an evaluation model of comprehensive development level of reclaimed water which consists of four layers with three criteria and 17 indicators is established. An improved catastrophe theory was utilized to obtain diversely distributed adjusted assessment values of comprehensive development level of reclaimed water. Finally, the single-factor sensitivity analysis was performed on the aforementioned model, and the sensitivity sequence of each indicator was determined according to the comprehensive development ability of reclaimed water. The results showed that 1. In Yunnan Province, the comprehensive development level of reclaimed water grew steadily from 2012 to 2015 but declined in 2016. Water deficiency was the most sensitive factor, followed by wastewater discharge, grade 1B treatment level, and construction impact as the secondary sensitive factors. 2. Compared with the fuzzy comprehensive evaluation method, the improved catastrophe evaluation method has the advantages of the rigorous theory, concise model, and simple calculation, and the decision result is more objective and reliable.
A Comparison of Fish Diversity in Rocky Reef Habitats by Multi-Mesh Gillnets and Environmental DNA Metabarcoding
Authors: Lin Yuan , Li Jun , Wang Zhenhua , Zhang Shouyu , Wang Kai , Li Xunmeng
Published: April 2022

This study was undertaken in order to explore the practical effectiveness of the environmental DNA (eDNA) metabarcoding approach in evaluating fish composition and diversity in a high heterogeneous rocky reef habitat. We assessed the fish composition and diversity characteristics of the rocky reef habitat at Dachen Islands, Taizhou and the Zhejiang Province in China in November 2020 by comparing two methods: multi-mesh gillnets and eDNA. A comparative analysis was carried out on the fish composition and diversity characteristics gained under the two methods by using taxonomy, ecotypes and diversity indices. The results showed that there were 28 species of fish collected through gillnets, distributed under 24 genera, 19 families, 6 orders and one class. Among them, 4, 18, and 6 species of near-surface, near groundfish and groundfish were found, respectively, with Thryssa mystax, Johnius belangerii, and Sebastiscus marmoratus being the dominant species in each water layer. A total of 81 species of fish detected by eDNA metabarcoding belonging to 67 genera, 46 families, 15 orders and 2 classes. The near-surface, near groundfish and groundfish species were 17, 42, and 22, with Thryssa vitrirostris, Benthosema pterotum, Harpadon nehereus, and Dasyatis akajei being the dominant species in each water layer. Twenty species (71.4%) and 41 species (50.6%) of reef fish were counted by gillnets and eDNA, respectively. The results showed that multi-mesh gillnets can accurately obtain information on fish composition in rocky reef habitats, but with some selectivity. The eDNA technology can detect species not collected by gillnets, but the number of species detected in areas with fast water velocity is significantly less than other eDNA stations where the water velocity is slow. In summary, the combination of traditional nets and eDNA will provide more information on taxonomic diversity and population biomass, transforming natural resource management and ecological studies of fish communities on a larger spatial and temporal scale.
MC4R Gene Polymorphisms Interact With the Urbanized Living Environment on Obesity: Results From the Yi Migrant Study
Authors: Wang Ye , Pan Li , Wan Shaoping , Yihuo Wuli , Yang Fang , He Huijing , Li Zheng , Yong Zhengping , Shan Guangliang
Published: April 2022

Objectives: This study aimed to determine the association of MC4R gene polymorphisms (rs17782313 and rs12970134) and urbanized living environment and the gene–environment interaction with obesity in Yi people in China.

Methods: A 1:2 frequency-matched case–control study based on the cross-sectional data was designed. Those with BMI ≥28 kg/m2 were included as the case group. The age- and sex-matched controls were selected from those with BMI <24 kg/m2. Unconditional logistic models were used to determine the association of SNPs with obesity. Additive interaction was evaluated by calculating relative excess risk due to interaction (RERI), attributable proportion due to interaction (AP), and synergy index (SI).

Results: A total of 322 cases and 643 controls were included. After adjustment, allele C of rs17782313 was significantly associated with obesity (additive model, OR = 1.52, 95%CI: 1.18–1.96) in Yi people. A similar association was found in allele A of rs12970134 (additive model, OR = 1.45, 95%CI: 1.13–1.89). Yi rural-to-urban migrants were found at 2.59-fold (95%CI: 1.70–3.95) higher odds of obesity than Yi farmers. Additive interactions were found between the two SNPs and rural-to-urban migration (rs17782313: AP = 0.65, 95%CI: 0.22–1.09; rs12970134: AP = 0.59, 95%CI: 0.02–1.17).

Conclusion:MC4R gene polymorphisms positively interacted with the urbanized living environment on obesity in Yi people. The effect of the MC4R gene on obesity was modified by the living environment.
Individualized Medicine in Africa: Bringing the Practice Into the Realms of Population Heterogeneity
Authors: Hussein Ayman A. , Hamad Reem , Newport Melanie J. , Ibrahim Muntaser E.
Published: April 2022

The declared aim of “personalized”, “stratified” or “precision” approaches is to place individual variation, as ascertained through genomic and various other biomarkers, at the heart of Scientific Medicine using it to predict risk of disease or response to therapy and to tailor interventions and target therapies so as to maximize benefit and minimize risk for individual patients and efficiency for the health care system overall. It is often contrasted to current practices for which the scientific base is rooted in concepts of a “universal biology” and a “typical” or “average patient” and in which variation is ignored. Yet both approaches equally overlook the hierarchical nature of human variation and the critical importance of differences between populations. Impact of genetic heterogeneity has to be seen within that context to be meaningful and subsequently useful. In Africa such complexity is compounded by the high effective size of its populations, their diverse histories and the diversity of the environmental terrains they occupy, rendering analysis of gene environment interactions including the establishment of phenotype genotype correlations even more cumbersome. Henceforth “Individualized” methods and approaches can only magnify the shortcomings of universal approaches if adopted without due regard to these complexities. In the current perspective we review examples of potential hurdles that may confront biomedical scientists and analysts in genomic medicine in clinical and public health genomics in Africa citing specific examples from the current SARS-COV2 pandemic and the challenges of establishing reference biobanks and pharmacogenomics reference values.
Identification of Diagnostic Biomarkers in Systemic Lupus Erythematosus Based on Bioinformatics Analysis and Machine Learning
Authors: Jiang Zhihang , Shao Mengting , Dai Xinzhu , Pan Zhixin , Liu Dongmei
Published: April 2022

Systemic lupus erythematosus (SLE) is a complex autoimmune disease that affects several organs and causes variable clinical symptoms. Exploring new insights on genetic factors may help reveal SLE etiology and improve the survival of SLE patients. The current study is designed to identify key genes involved in SLE and develop potential diagnostic biomarkers for SLE in clinical practice. Expression data of all genes of SLE and control samples in GSE65391 and GSE72509 datasets were downloaded from the Gene Expression Omnibus (GEO) database. A total of 11 accurate differentially expressed genes (DEGs) were identified by the “limma” and “RobustRankAggreg” R package. All these genes were functionally associated with several immune-related biological processes and a single KEGG (Kyoto Encyclopedia of Genes and Genome) pathway of necroptosis. The PPI analysis showed that IFI44, IFI44L, EIF2AK2, IFIT3, IFITM3, ZBP1, TRIM22, PRIC285, XAF1, and PARP9 could interact with each other. In addition, the expression patterns of these DEGs were found to be consistent in GSE39088. Moreover, Receiver operating characteristic (ROC) curves analysis indicated that all these DEGs could serve as potential diagnostic biomarkers according to the area under the ROC curve (AUC) values. Furthermore, we constructed the transcription factor (TF)-diagnostic biomarker-microRNA (miRNA) network composed of 278 nodes and 405 edges, and a drug-diagnostic biomarker network consisting of 218 nodes and 459 edges. To investigate the relationship between diagnostic biomarkers and the immune system, we evaluated the immune infiltration landscape of SLE and control samples from GSE6539. Finally, using a variety of machine learning methods, IFI44 was determined to be the optimal diagnostic biomarker of SLE and then verified by quantitative real-time PCR (qRT-PCR) in an independent cohort. Our findings may benefit the diagnosis of patients with SLE and guide in developing novel targeted therapy in treating SLE patients.
The Human Male Liver Is Predisposed to Inflammation Via Enhanced Myeloid Responses to Inflammatory Triggers
Authors: Kuipery Adrian , Mahamed Deeqa , Nkongolo Shirin , D’Angelo June Ann , Johnson Valiente Alexandra , Mehrotra Aman , Chapman William C. , Horton Peter , McGilvray Ian , Janssen Harry L. A. , Gehring Adam J.
Published: April 2022

Background & Aim

Men have a higher prevalence of liver disease. Liver myeloid cells can regulate tissue inflammation, which drives progression of liver disease. We hypothesized that sex alters the responsiveness of liver myeloid cells, predisposing men to severe liver inflammation.

Methods

Luminex was done on plasma from Hepatitis B Virus infected patients undergoing nucleoside analogue cessation in 45 male and female patients. We collected immune cells from the sinusoids of uninfected livers of 53 male and female donors. Multiparametric flow cytometry was used to phenotype and characterize immune composition. Isolated monocytes were stimulated with TLR ligands to measure the inflammatory potential and the expression of regulators of TLR signaling.

Results

We confirmed that men experienced more frequent and severe liver damage upon Hepatitis B Virus reactivation, which was associated with inflammatory markers of myeloid activation. No differences were observed in the frequency or phenotype of sinusoidal myeloid cells between male and female livers. However, monocytes from male livers produced more inflammatory cytokines and chemokines in response to TLR stimulation than female monocytes. We investigated negative regulators of TLR signaling and found that TOLLIP was elevated in female liver-derived monocytes

Conclusions

Our data show that enhanced responsiveness of myeloid cells from the male liver predisposes men to inflammation, which was associated with altered expression of negative regulators of TLR signaling.
Alterations in Fetal Doppler Parameters Before and Twenty-Four Hours After Radiofrequency Ablation for Twin Reversed Arterial Perfusion Sequence
Authors: Zhang Lan , Liu Hongli , Huang Shuai , Tong Chao , Wang Zhigang , Qi Hongbo , Baker Philip N. , Kilby Mark D.
Published: April 2022

Objective

To evaluate alterations in the fetal Doppler parameters of pump fetuses before and 24 h after radiofrequency ablation surgery for twin reversed arterial perfusion sequence (TRAPs).

Methods

This is a retrospective study of 28 pump fetuses in TRAPs and 28 normal control twins between 2016 and 2021. The fetal Doppler parameters, including the umbilical artery pulsatility index (UA-PI), middle cerebral artery peak systolic velocity (MCA-PSV), middle cerebral artery pulsatility index (MCA-PI), and cerebroplacental ratio (CPR), of the controls, and pump fetuses before and 24 h after surgery were compared.

Results

An increasing trend and a further increase in the MCA-PSV, MCA-PI, MCA-PSV Z score, and MCA-PI Z score after surgery were observed in pump fetuses with gestational age (GA) ≥20 weeks; however, such changes were not observed in those with a GA of <20 weeks. The UA-PI and CPR before and after surgery were not different between control and pump fetuses, whether the GA was ≥20 or <20 weeks.

Conclusion

In the middle second trimester, the pump fetus might suffer from high cardiac output rather than hypoxemia before surgery and congestive heart failure, or hemodilutional anemia after surgery. This may provide some theoretical evidence in favor of early intervention, rather than waiting for a more advanced GA, to avoid unnecessary hemodynamic alterations.
Surgical Masks Affect the Peripheral Oxygen Saturation and Respiratory Rate of Anesthesiologists
Authors: Yang Shaozhong , Fang Chuanyu , Liu Xin , Liu Yu , Huang Shanshan , Wang Rui , Qi Feng
Published: April 2022

Background

Surgical masks (SMs) protect medical staff and reduce surgical site infections. Extended SM use may reduce oxygen concentrations in circulation, causing hypoxia, headache, and fatigue. However, no research has examined the effects of wearing SMs on oxygenation and physical discomfort of anesthesiologists.

Methods

An electronic questionnaire was established and administered through WeChat, and a cross-sectional survey was conducted to determine SM use duration and related discomfort of operating room medical staff. Then, operating room anesthesiologists were enrolled in a single-arm study. Peripheral blood oxygen saturation (SpO2), heart rate, and respiratory rate were determined at different times before and after SM use. Shortness of breath, dizziness, and headache were subjectively assessed based on the visual analog scale (VAS) scores.

Results

In total, 485 operating room medical staff completed the electronic questionnaire; 70.5% of them did not change SMs until after work, and 63.9% wore SMs continuously for more than 4 h. The proportion of anesthesiologists was the highest. After wearing masks for 4 h, the shortness of breath, fatigue, and dizziness/headache rates were 42.1, 34.6, and 30.9%, respectively. Compared with other medical staff, the proportion of subjective discomfort of anesthesiologists increased significantly with prolonged SM use from 1 to 4 h. Thirty-five anesthesiologists completed the study. There was no difference in anesthesiologist SpO2, heart rate, or respiratory rate within 2 h of wearing SMs. After more than 2 h, the variation appears to be statistically rather than clinically significant—SpO2 decreased (98.0 [1.0] vs. 97.0 [1.0], p < 0.05), respiratory rate increased (16.0 [3.0] vs. 17.0 [2.0], p < 0.01), and heart rate remained unchanged. As mask use duration increased, the VAS scores of shortness of breath, dizziness, and headache gradually increased.

Conclusion

In healthy anesthesiologists, wearing SMs for more than 2 h can significantly decrease SpO2 and increase respiratory rates without affecting heart rates.
Study Protocol: A Randomized Controlled Prospective Single-Center Feasibility Study of Rheopheresis for Raynaud’s Syndrome and Digital Ulcers in Systemic Sclerosis (RHEACT Study)
Authors: Rademacher Jan-Gerd , Tampe Björn , Borisch Angela , Buschfort Rosa Marie , von Figura Andrea , Asendorf Thomas , Korsten Peter
Published: April 2022

Introduction

Raynaud’s phenomenon (RP) and digital ulcers (DU) are frequent manifestations of Systemic Sclerosis (SSc). Despite being very common in SSc patients, both conditions have proven to be notoriously difficult to study. There are very few available approved drugs with varying efficacy. It has been shown that the presence of DU is associated with increased whole blood viscosity (WBV). Rheopheresis (RheoP) is an extracorporeal apheresis technique used to treat microcirculatory disorders by improving blood viscosity. Improved blood flow and wound healing after RheoP treatments have been reported in single case reports.

Methods and Analysis

We report the clinical trial protocol of “A randomized controlled prospective single-center feasibility study of Rheopheresis for Raynaud’s syndrome and Digital Ulcers in Systemic Sclerosis (RHEACT).” RHEACT aims to investigate the efficacy of RheoP on the Raynaud Condition Score (RCS) as the primary efficacy outcome measure after 16 weeks from baseline. Thirty patients will be randomized in a 1:1:1 ratio to one of two RheoP treatment groups or assigned to the standard of care (SoC) control group (intravenous iloprost). Secondary endpoints include changes in DU, changes in nailfold video capillaroscopy and patient-reported-outcomes (Scleroderma Health Assessment Questionnaire, FACIT-Fatigue, and the Disability of Arm, Shoulder, and Hand, quick version).

Discussion

Apheresis techniques have been investigated in SSc but mainly in observational, retrospective studies, or single case reports. RheoP is a pathophysiologically driven potential new therapy for heavily burdened patients with SSc-associated secondary RP with or without DU.

Ethics and Dissemination

The study was registered at clinicaltrials.gov (Identifier: NCT05204784). Furthermore, the study is made publicly available on the website of the German network of Systemic Sclerosis “Deutsches Netzwerk Systemische Sklerodermie (DNSS).”
Changes in Rats’ Gut Microbiota Composition Caused by Induced Chronic Myocardial Infarction Lead to Depression-Like Behavior
Authors: Wang Qianwen , Wang Xi , Lv Yong , Yang Chun , Zhou Chenliang , Wang Long
Published: April 2022

Depression is common among patients who have chronic myocardial infarction (CMI). Despite their frequency, depression and CMI are bidirectional related conditions, each is a risk for the other, and they often co-exist, suggesting shared or interacting pathomechanisms. Accumulating data revealed the effects of gut microbiota in terms of regulating depression via the gut–brain axis. Thus, we investigated the role of gut microbial dysbiosis in CMI-induced depression-like behavior. Hierarchical cluster analysis of sucrose preference test (SPT) results was adopted to classify the CMI rats into depression-like behavior (CMI + Dep) or non-depression-like behavior (CMI + Non-Dep) phenotypes. First, 16S ribosomal RNA sequencing analysis showed both β-diversity and relative abundance of several gut bacteria significantly differed between the CMI + Dep and CMI + Non-Dep rats. Next, transplantation of fecal microbiota from CMI + Dep rats visibly altered the relative abundance of gut microbiota and also induced depression-like behavior in the antibiotics-treated pseudo-germ-free rats. In conclusion, these findings suggested that dysbiosis in gut microbial composition contributed to the onset of CMI-induced depression-like behavior and that exogenous regulation of gut microbiota composition could be a potential therapeutic strategy for CMI and related depression-like behavior.
Suggested Sustainable Medical and Environmental Uses of Melanin Pigment From Halotolerant Black Yeast Hortaea werneckii AS1
Authors: Elsayis Asmaa , Hassan Sahar W. M. , Ghanem Khaled M. , Khairy Heba
Published: April 2022

The marine ecosystem is a complex niche with unique environmental circumstances. Microbial communities from the sea are one of the main origins of compounds with tremendous capabilities. Marine yeasts have the ability to produce secondary metabolites that are architecturally distinct from those found in terrestrial species. Melanin pigment synthesized by marine halotolerant black yeastHortaea werneckiiAS1 isolated from Mediterranean salt lakes in Alexandria, Egypt was found to exert a radical scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) with an IC50of 61.38 μg/ml. Furthermore, it showed no cytotoxicity toward human skin fibroblast cell line (HSF) with an IC50value above 0.1 mg/ml. The antimicrobial capability of the pigment was revealed against the tested number of bacterial and fungal strains with the highest inhibition zone of 25 mm againstAeromonassp. and a growth inhibition percentage up to 63.6% againstAspergillus niger. From an environmental impact point of view, the pigment disclosed a heavy metal removal efficiency of 85.7, 84.8, and 81.5% for Pb2+, Cd2+, and Ni2+, respectively, at 100 mg/L metal concentration. The previously mentioned results suggested melanin fromH. werneckiiAS1 as a promising biocompatible candidate in various medical, cosmetics, pharmaceutical, and environmental applications.
Enzyme Promiscuity in Serotonin Biosynthesis, From Bacteria to Plants and Humans
Authors: Gonçalves Sara , Nunes-Costa Daniela , Cardoso Sandra Morais , Empadinhas Nuno , Marugg John David
Published: April 2022

Serotonin is a phylogenetically ancient compound found in animals, plants, and some bacteria. In eukaryotes, serotonin is synthesized from the aromatic amino acid tryptophan via the key enzymes aromatic amino acid hydroxylase (AAAH) and aromatic amino acid decarboxylase (AAAD). Serotonin is also an intermediate in the melatonin biosynthetic pathway and is involved in several vital functions. In humans, serotonin is produced in the gut and in the brain, is critical in the regulation of multiple body functions, and its depletion has been implicated in multiple neurological disorders including depression and Alzheimer’s disease, as well as other peripheral conditions namely irritable bowel syndrome and fibromyalgia. The serotonin biosynthetic pathway is well described in eukaryotes, but very little is known about this pathway in bacteria. Evidence points to similar pathways since eukaryote-like AAAH and AAAD (and their genes) have been identified in multiple bacteria, even though serotonin production has not yet been detected in most species. Although data on bacterial tryptophan decarboxylase genes are very limited and no bacterial tryptophan hydroxylase genes have been identified to date, evidence suggests that serotonin production in bacteria might occur through different AAAH and AAAD. Substrate promiscuity in these enzymes has been previously reported and seems to be the key aspect in bacterial serotonin synthesis. Considering the human gut microbiota as a potential source of serotonin, further investigation on its biosynthetic pathways in microbes might lead to important discoveries, which may ultimately foster the development of new therapeutic strategies to treat serotonin depletion-related disorders in humans.
A Neuron, Microglia, and Astrocyte Triple Co-culture Model to Study Alzheimer’s Disease
Authors: Luchena Celia , Zuazo-Ibarra Jone , Valero Jorge , Matute Carlos , Alberdi Elena , Capetillo-Zarate Estibaliz
Published: April 2022

Glial cells are essential to understand Alzheimer’s disease (AD) progression, given their role in neuroinflammation and neurodegeneration. There is a need for reliable and easy to manipulate models that allow studying the mechanisms behind neuron and glia communication. Currently available models such as co-cultures require complex methodologies and/or might not be affordable for all laboratories. With this in mind, we aimed to establish a straightforward in vitro setting with neurons and glial cells to study AD. We generated and optimized a 2D triple co-culture model with murine astrocytes, neurons and microglia, based on sequential seeding of each cell type. Immunofluorescence, western blot and ELISA techniques were used to characterize the effects of oligomeric Aβ (oAβ) in this model. We found that, in the triple co-culture, microglia increased the expression of anti-inflammatory marker Arginase I, and reduced pro-inflammatory iNOS and IL-1β, compared with microglia alone. Astrocytes reduced expression of pro-inflammatory A1 markers AMIGO2 and C3, and displayed a ramified morphology resembling physiological conditions. Anti-inflammatory marker TGF-β1 was also increased in the triple co-culture. Lastly, neurons increased post-synaptic markers, and developed more and longer branches than in individual primary cultures. Addition of oAβ in the triple co-culture reduced synaptic markers and increased CD11b in microglia, which are hallmarks of AD. Consequently, we developed a straightforward and reproducible triple co-cultured model, where cells resemble physiological conditions better than in individual primary cultures: microglia are less inflammatory, astrocytes are less reactive and neurons display a more mature morphology. Moreover, we are able to recapitulate Aβ-induced synaptic loss and CD11b increase. This model emerges as a powerful tool to study neurodegeneration and neuroinflammation in the context of AD and other neurodegenerative diseases.
Regional Homogeneity in Patients With Mild Cognitive Impairment: A Resting-State Functional Magnetic Resonance Imaging Study
Authors: Wu Yu-Qian , Wang Yi-Ning , Zhang Li-Juan , Liu Li-Qi , Pan Yi-Cong , Su Ting , Liao Xu-Lin , Shu Hui-Ye , Kang Min , Ying Ping , Xu San-Hua , Shao Yi
Published: April 2022

Objective

To analyze the potential changes in brain neural networks in resting state functional magnetic resonance imaging (rs-fMRI) scans by regional homogeneity (ReHo) in patients with mild cognitive impairment (MCI).

Methods

We recruited and selected 24 volunteers, including 12 patients (6 men and 6 women) with MCI and 12 healthy controls matched by age, sex, and lifestyle. All subjects were examined with rs-fMRI to evaluate changes in neural network connectivity, and the data were analyzed by ReHo method. Correlation analysis was used to investigate the relationship between ReHo values and clinical features in different brain regions of MCI patients. The severity of MCI was determined by the Mini-Mental State Examination (MMSE) scale.

Results

The signals of the right cerebellum areas 4 and 5, left superior temporal, right superior temporal, left fusiform, and left orbital middle frontal gyri in the patient group were significantly higher than those in the normal group (P < 0.01 by t-test of paired samples). The signal intensity of the right inferior temporal and left inferior temporal gyri was significantly lower than that of the normal group (P < 0.01). The ReHO value for the left inferior temporal gyrus correlated negatively with disease duration, and the value for the right inferior temporal gyrus correlated positively with MMSE scores.

Conclusion

Mild cognitive impairment in patients with pre- Alzheimer’s disease may be related to the excitation and inhibition of neural networks in these regions. This may have a certain guiding significance for clinical diagnosis.
Non-Invasive Neuromodulation in the Rehabilitation of Pisa Syndrome in Parkinson's Disease: A Randomized Controlled Trial
Authors: De Icco Roberto , Putortì Alessia , Allena Marta , Avenali Micol , Dagna Carlotta , Martinelli Daniele , Cristina Silvano , Grillo Valentina , Fresia Mauro , Bitetto Vito , Cosentino Giuseppe , Valentino Francesca , Alfonsi Enrico , Sandrini Giorgio , Pisani Antonio , Tassorelli Cristina
Published: April 2022

Background

Pisa syndrome (PS) is a frequent postural complication of Parkinson's disease (PD). PS poorly responds to anti-parkinsonian drugs and the improvement achieved with neurorehabilitation tends to fade in 6 months or less. Transcranial direct current stimulation (t-DCS) is a non-invasive neuromodulation technique that showed promising results in improving specific symptoms in different movement disorders.

Objectives

This study aimed to evaluate the role of bi-hemispheric t-DCS as an add-on to a standardized hospital rehabilitation program in the management of PS in PD.

Methods

This study included 28 patients with PD and PS (21 men, aged 72.9 ± 5.1 years) who underwent a 4-week intensive neurorehabilitation treatment and were randomized to receive: i) t-DCS (t-DCS group, n = 13) for 5 daily sessions (20 min−2 mA) with bi-hemispheric stimulation over the primary motor cortex (M1), or ii) sham stimulation (sham group, n = 15) with the same duration and cadence. At baseline (T0), end of rehabilitation (T1), and 6 months later (T2) patients were evaluated with both trunk kinematic analysis and clinical scales, including UPDRS-III, Functional Independence Measure (FIM), and Numerical Rating Scale for lumbar pain.

Results

When compared to the sham group, the t-DCS group achieved a more pronounced improvement in several variables: overall posture (p = 0.014), lateral trunk inclination (p = 0.013) during upright standing position, total range of motion of the trunk (p = 0.012), FIM score (p = 0.048), and lumbar pain intensity (p = 0.017).

Conclusions

Our data support the use of neuromodulation with t-DCS as an add-on to neurorehabilitation for the treatment of patients affected by PS in PD.