bluuebunny/crossref_metadata_2025 huggingface dataset:
Antioxidant Components and Antioxidant Activities of Hydroglyceroalcoholic Extract from Fresh Mushroom Mycelium
Authors: Vamanu Emanuel
Published: February 2020

The in vitro antioxidant activity, as well as the hydroglyceroalcoholic extracts main active components from mycelia of Pleurotus ostreatus and Coprinus comatus, were determined. The mycelium submitted for extraction was obtained by submerged cultivation in a bioreactor. The hydroglyceroalcoholic extracts, in particular, those of P. ostreatus PSI101109 and PQMZ91109 mycelium had a considerable antioxidant activity, and was compared to the overall constituent of flavonoids plus phenolic compounds. According to the extracts HPLC assessment, the key phenolic acid evident from the extracts was gentisic acid. The homogentisic, gallic and protocatechuic acids were also determined. Catechin was identified as well, except for C. comatus M8102 and P. ostreatus PBS281009 mycelia extracts. Since catechol and gentisic acid levels were high, respectively, there was a correlation with the absence of carotenoid compounds in hydroglyceroalcoholic extracts (P. ostreatus PSI101109). There is a correspondence between the hydroglyceroalcoholic extracts� key antioxidant activities with the catechin and polyphenolic acids levels identified in each extract.
Urinary Tract Infections in Patients with Type 1 and Type 2 Diabetes: Etiology, Resistance to Antibacterial Chemicals and Virulence Features
Authors: Mitache Mihaela Magdalena , Curutiu Carmen , Rusu Elena , Bahna Ramona , Ditu Mara , Moldovan Horatiu , Hancu Violeta , Chifiriuc Mariana Carmen
Published: February 2020

One of the most frequent chronic complications occurred in diabetes patients are the urinary tract infections (UTIs). This study aimed to investigate the incidence of UTIs in a cohort of 93 (47 males: 46 females) diabetic patients, the prevalence of different microbial species involved and their virulence and antibiotic resistance profiles. The identification of the uropathogenic strains in the positive urine samples was performed using conventional methods and API tests. After identification, the antibiotic susceptibility profiles were established by the standardized disk diffusion method and double disk diffusion test was performed for the confirmation of ESBL and inducible AmpC b �lactamase phenotypes. The isolated strains were tested for the production of different cell associated and soluble virulence factors, i.e.: bacterial adherence to cellular substrata (HeLa cells), hemolysins (hemolysis spot, CAMP-like), amylase, caseinase, aesculin hydrolysis, DNA-ase, lipase and lecithinase. In the analyzed group, the total prevalence of UTIs was of 46%, a higher incidence being observed in the female patients (64%). Similar to other studies, the etiology of UTI in the investigated diabetes patients was dominated by E. coli, followed by Klebsiella sp. strains. The isolated strains preserved good susceptibility rates to quinolones and aminoglycosides and revealed important virulence features, related to their capacity to colonize the cellular substratum and to produce soluble virulence factors involved in persistence, colonization and progression of the infectious process. The high percentage of beta-lactam resistant strains (including carbapenem-resistant ones) requires careful surveillance of the dynamics of susceptibility profiles for limiting the emergence of these strains in community.
Study of Utilization of Agricultural Waste as Environmental Issue in Romania
Authors: Ungureanu George , Ignat Gabriela , Vintu Catalin Razvan , Diaconu Constantin Daniel , Sandu Ioan Gabriel
Published: February 2020

Agriculture and animal husbandry produce significant quantity of solid or liquid residues and waste products. Unfortunately, some of these wastes are not dealt with properly and are causing considerable damage to the environment. Agricultural wastes (AW) in Romania amount range from 7600 thousand tons a year of which only 1400 thousand tons as animal feed and 1100 thousand tons as organic manure are being utilized. These crop waste results after harvesting in the farm of leaves stem which are characterized as coarse plant by-products and big size, chemically low in protein and fat contents, or, like other country (example: Netherlands) avoid waste as much as possible, recover the valuable raw materials from any waste that is created, try to generate energy by incinerating the residual waste, and only then dump what is left. The focus of the research paper is to investigate the importance of agriculture wastes that becomes very obvious and aggregated after the harvest crops. The most common solution is the utilizations of agricultural waste for compositing, as animal fodder, most often as a source of energy, food production, by growing mushroom on agricultural wastes such as oat straw as a substrate. This means the conversion of wastes to economic, nutritional human food. Growing vegetables on oat straw compacted bales in areas where soil disease and salinity are constrains. The implementation of most of the solutions to agricultural waste management does not meet the basic elements of sustainability like environmental protection and social progression, technical and technological improvement as well as economic improvements.
Bituminous Insulations Durability of Underground Metallic Pipelines I Field investigations
Authors: Oprina Gabriela , Radermacher Ladislau , Lingvay Daniel , Marin Dorian , Voina Andreea , Mitrea Sorina
Published: February 2020

The corrosion state of an underground metallic pipeline of �161 mm and 565 m length was assessed by specific electrical and electrochemical measurements. The investigated pipe, buried in 1997, was protected against corrosion by successive layers of bituminous material with a total thickness of 1 to 1.2 mm. The pipeline crosses three electrified railway lines (50 Hz - 28 kV), and then its route is approximately parallel to these lines; thus, the induced AC voltages between line and ground were calculated obtaining values between 4.05 and 7.1 Vrms, in good agreement with the values measured in the accessible points. The measurements regarding the insulation capacity against corrosion of the bituminous insulation, performed at one month and after 19 years of burial, showed an increase of the average cathode current density needed for obtaining the protection potential in the range �1.00 � �1.28 VCu/CuSO4 of approx. three times (from 6.65 up to 19.96 mA/m2), in good agreement with the evolution of the insulation resistance measured between the steel pipe (having a contact area with the ground of 270.5 m2) and a ground socket of 4 W, which decreased from 995 to 315 kW. Following the analysis and processing of the field collected data, it is considered that, by implementing a cheap cathodic protection system (without cathodic current power supply), based on the rectification of the AC induced voltage, the safe operation period of the investigated pipeline may be extended by at least 50 years.
Efficeint Administration of Human Albumin in Clostridium Difficile Infection
Authors: Draganescu Miruna , Baroiu Nicusor , Baroiu Liliana , Diaconu Camelia , Dumitriu Buzia Olimpia
Published: February 2020

Albumin is the most important protean component of the plasma. Human albumin is a concentrate of plasmatic proteins from the blood with multiple therapy uses. The study aims to highlights the efficient administration of human albumin i.v. in hypoalbuminemia due to diarrhea with Clostridium difficile. Administering human albumin i.v. in symptomatic hypoalbuminemia manifested by the occurrence of edemas was followed by symptoms remission and ensured a favorable evolution.
The Biochemical Effects of Non-surgical Periodontal Therapy in Patients with and without Chronic Renal Disease
Authors: Sincar Cerasela Dorina , Ioanid Nicoleta , Rudnic Ioana , Martu Ioana , Solomon Sorina Mihaela , Pavel Liliana Lacramioara , Rezus Ciprian , Martu Silvia , Plesea Condratovici Catalin
Published: February 2020

Recent studies have shown an association between high levels of biomarkers and periodontitis, association which decreases after the periodontal treatment. Because of this association with systemic inflammatory response, chronic periodontitis was recently included as a risk factor for chronic renale disease. Specifically, in this study we hypothesized that a part of chronic inflammatory response observed in patients with chronic renale disease is due to physiopathological reactions caused by the presence of chronic periodontitis, which, during the course of its evolution, induces an increase in the expression of inflammatory markers. Patients were divided into two groups: the first group consisted of patients with chronic renale disease and periodontal disease who were undergoing periodontal treatment (test group) and the second group, control group, composed of patients without any systemic disease, but who experienced moderate to severe chronic periodontitis, also periodontal treated. Blood samples were taken for biochemical analysis at baseline and at 3 months after periodontal therapy. Venous blood was collected in vacuum tubes between 7:00 am and 9:00 am after 12 hours after the last meal. A tube containing EDTA was analyzed for blood following parameters: albumin, uric acid, creatinin, urea. An association between periodontal disease and renale disease is often found in studies using a population where the renale disease is already diagnosed. In these cases, duration of renal end stage and type of topical and systemic treatment administered to patients significantly affect the association. Therefore, we have shown that periodontitis may promote any detectable changes in renal function. Thus, by analogy, in this study the test groups and control groups were compared not only with each other but also comparative analyzes were performed based on the reference values of markers of renal dysfunction. We think it could be plausible existence of a causal link between periodontal disease and chronic renal disease both by glomerular invasion by periodontal pathogens, directly and indirectly through systemic inflammatory effect caused by chronic periodontitis. Success of periodontal therapy reduce systemic inflammatory response and decreases levels of biochemicals markers indicating that this may be an important intervention therapy in patients with chronic renale disease.
Efficiency of Tranexamic Acid in Management of Surgical Orthopedic Bleeding in Patients with Haemophilia
Authors: Badulescu Oana Viola , Tudor Razvan , Friedl Wilhelm , Blaj Mihaela , Sirbu Paul Dan
Published: February 2020

Haemophilia is an inherited bleeding disorder (gonosomala recessive, related to chromosome X, with transmission from carrying women to male descendents) characterised from the clinic point of view by important bleeding, secondary to some minimum and biologic traumas by deficiency of trombo-plastino-formation, consecutive to either a deficit of factor VIII (haemophilia A), or the factor IX (haemophilia B). The most characteristic manifestation of hemophilia is intra-articular � hemarthrosis. Its repetitive character leads to irreversible lesions of the articular structures, inducing lesions of the synovium with degenerative effects over the articular cartilage and destructive effects for the subchondral bone tissue. In time, these lesions require orthopaedic surgery to improve the locomotor activity. Managing an efficient hemostasis is vital during surgery, due to high risk of bleeding triggered by coagulopathy and surgery. Numerous studies carried out underlined the efficiency of the tranexamic acid (TXA) in reducing bleeding, in different surgery branches, by inhibiting the enzymatic degradation of fibrin. In orthopaedic surgery, the tranexamic acid is frequently used in case of hip and knee arthroplasties, reducing the bleeding and blood transfusion necessary to the treatment of posthaemorrhagic anemia. This paper wants to assess the efficiency of the tranexamic acid in realization of hemostasis to another category of patients, haemophiliac patients with indication of total hip and knee endoprosthesis.
Fast Method for the Determination of Residual Solvents in Radiopharmaceutical Products
Authors: Mihon Mirela , Tuta Catalin Stelian , Ion Alina Catrinel , Niculae Dana , Lavric Vasile
Published: February 2020

The aim of this work was the development and validation of a fast analytical method to determine the residual solvents content in radiopharmaceuticals such as: 18F-Fluorodeoxyglucose (18F-FDG), 18F-Fluoroestradiol (18F-FES), 18F-Fluorothymidine (18F-FLT),18F-Fluoromisonidazole (18F-FMISO). Radiopharmaceuticals are radioactive preparations for medical purposes used in nuclear medicine as tracers in diagnostic imaging and treatment of certain diseases. Positron Emission Tomography (PET) is a medical imaging technique that consists in introducing into the body of a small amount of a biologically active chemical compound labelled with a short lived positron-emitting radioisotope (18F, 11C, 68Ga). Residual solvents are critical impurities in radiopharmaceuticals that can affect labelling, stability and physicochemical properties of drugs. Therefore, the determination of these solvents is essential for quality control of radiopharmaceuticals. Validation of the control method for residual solvents by gas chromatography is referred by the European Pharmacopoeia using a special injection technique (head space). The parameters of the method, which comply with International Conference on Harmonization guidelines, are: accuracy, precision, linearity, limit of detection, limit of quantification and robustness. The proposed method (direct gas chromatography injection) proved to be linear, precise, accurate and robust. Good linearity was achieved for all the solvents and correlation coefficients (R2) for each residual solvent were found more than 0.99.
Study on Chemical Composition of Urinary and Salivary Gland Stones in Relationship with Laboratory Parameters and Lifestyle Habits of Patients with Lithiasis
Authors: Nemes Nagy Eniko , Tilinca Mariana Cornelia , Iacob Alina , Ormenisan Alina , Fazakas Zita , Barbu Horia Mihail , Kolcsar Melinda , Maier Adrian Cornel , Vida Arpad Oliver , Orsolya Martha
Published: February 2020

Lithiasis is a disease with increasing incidence, may occur in the urinary tract, salivary glands and bile ducts. Genetic susceptibility, diet, low fluid intake, endocrine disorders, infections and other factors can influence the development of the disease and its recurrences. The aim of the study was to reveal the relationship between chemical composition of the stones, lifestyle and dietary habits and the results of laboratory analysis in patients with urinary tract and salivary gland lithiasis. The data of 258 patients with urinary tract and salivary gland lithiasis collected between September 2009 � May 2016 were studied. The subjects were from the Urology and Oral Surgery Hospital, and from the Marmed and Procardia medical units in Tirgu Mures. We evaluated the lifestyle habits using a questionnaire, microscopical examination, size measurement and chemical analysis of the stones were made and we processed the results of urinary strip analysis and sediment. In a subgroup of patients (48 samples) calciuria, phosphaturia, serum uric acid, calcium, phosphate, alkaline phosphatase and parathormone (PTH) levels were determined; in case of pathological values calcitonin measurement and endocrinology consultation were performed. The most frequent composition was the combination of calcium oxalate and phosphate in case of both urinary and salivary gland stones, in several uroliths we detected uric acid/xanthine crystals, rarely cystine and carbonate were found. The incidence of hematuria and leukocyturia exceeded 70%. Excessive calcium intake was observed in the diet of the several patients having calcium oxalate stones. In the subgroup tested for biochemical and hormonal analyses we found increased serum PTH concentration in 20.83% of the patients. Some of the subjects had pathological serum calcium, uric acid and alkaline phosphatase results, or the elimination of minerals in the urine was out of the normal range. We found pathological laboratory results in several patients suffering from lithiasis. Proper diet and medical treatment in many cases could prevent the recurrences of stone formation, but first a complex investigation of the patients is necessary to adapt the treatment plan to individual requirements.
Fuels Desulphurization by Adsorption on Blasting Grit
Authors: Ion Constantin Sorin , Bombos Mihaela , Vasilievici Gabriel , Panaitescu Casen , Dragomir Raluca
Published: February 2020

Desulphurization by reactive adsorption was studied on sand abrasive blasting grit/ bentonite. The adsorbent was characterized by determining the composition, adsorption isotherms, specific surface area, pore volume and average pore diameter. Adsorption experiments were performed in continuous system at 260�300oC, 25 atm and volume hourly space velocities of 1-2 h-1. The efficiency of adsorption was evaluated at desulphurization of a synthetic reaction mixture and a catalytic cracking gas oil.
Synthesis, Characterization and Antiproliferative Activity Assessment of a Novel 1H-5-mercapto-1,2,4 Triazole Derivative
Authors: Mioc Marius , Avram Sorin , Bercean Vasile , Balan Porcarasu Mihaela , Soica Codruta , Susan Razvan , Kurunczi Ludovic
Published: February 2020

Angiogenesis plays an important function in tumor proliferation, one of the main angiogenic promoters being the vascular endothelial growth factor (VEGF) which activates specific receptors, particularly VEGFR-2. Thus, VEGFR-2 has become an essential therapeutic target in the development of new antitumor drugs. 1,2,4-triazoles show a wide range of biological activities, including antitumor effect, which was documented by numerous reports. In the current study the selection of 5-mercapto-1,2,4-triazole structure (1H-3-styryl-5-benzylidenehydrazino-carbonyl-methylsulfanil-1,2,4-triazole, Tz3a.7) was conducted based on molecular docking that emphasized it as suitable ligand for VEGFR-2 and EGFR1 receptors. Compound Tz3a.7 was synthesized and physicochemically and biologically evaluated thus revealing a moderate antiproliferative activity against breast cancer cell line MDA-MB-231.
The Impact of Water Activity on Storage Stability of a Newly Reformulated Salami A pilot scale study
Authors: Tudose Cristian , Neagu Corina , Borda Daniela , Alexe Petru
Published: February 2020

Two formulations of salami were obtained: a conventional salami (50 % beef, 20 % pork and 30 % animal fat, w/w) and a reformulated one (50 % beef, 20 % pork and 30 % a mixture of olive and palm oil - 2.5:1, w/w). Storage studies were performed by accelerated shelf life testing at 20�C and different relative humidity, in comparison with 4 and 8�C after 22 days of storage. Halsey model was the best one to describe the desorption isotherms. The products stability was evaluated by TBA (thiobarbituric acid) index and colour changes. TBA increased with the increase of the environment relative humidity at 4 and 8�C. Modified Weibull model described the changes in colour as a function of TBA and water activity. Bound water characteristics have markedly changed at 20�C for both products. The results of this work can be used as reference in defining reformulated salami minimal durability.
Assessment of Mitochondrial Respiration in Human Platelets
Authors: Petrus Alexandra , Ratiu Corina , Noveanu Lavinia , Lighezan Rodica , Rosca Mariana , Muntean Danina , Duicu Oana
Published: February 2020

It has been long recognized that the impairment of platelet mitochondrial function occurs in a broad spectrum of diseases. Accordingly, the assessment of platelet respiratory dys/function has emerged as a putative approach allowing the characterization of the early impairment of human bioenergetic profile in several chronic pathologies. The aim of this study was to standardize the methodology for platelet isolation from peripheral blood and the measurement of mitochondrial oxygen consumption by means of high-resolution respirometry, respectively. The platelet isolation protocol consisted of two consecutive centrifugations of the whole blood collected from adult healthy females (n = 10) yielding a platelet-rich plasma sample. Respiration was measured at 370C using the Oxygraph-2k (Oroboros Instruments, Austria) according to a classic substrate-uncoupler-inhibitor-titration protocol. Platelets permeabilized with digitonin were allowed to respire in the presence of complex I (glutamate and malate) and complex II (succinate) substrates. We obtained a respiratory control ratio of 2.77 � 3.65 that indicates an accurate coupling efficiency of oxidative phosphorylation. The in vitro measurement of platelet respiration is a reliable method to evaluate the bioenergetic profile in humans. The standardized technique will be further used to assess the occurrence of mitochondrial dysfunction in peripheral blood in the setting of various chronic non-communicable diseases.
The Effect of ortho-Phosphoric Acid Etching Application on Enamel Surface ATR-FTIR and SEM studies
Authors: Murariu Alice , Zaltariov Mirela , Vasiliu Lavinia , Balan Adriana , Savin Carmen , Gavrila Laura Maria , Forna Norina Consuela
Published: February 2020

The acid etching procedure represents a procedure that increases the bond strength between the etched enamel and the composite resin. The aim of this study was to evaluate the effect of ortho-phosphoric acid 37% on the morphology and composition of the enamel surface. The surfaces of four extracted teeth (two incisors and two premolars) were etched with 37% ortho-phosphoric acid solution for 15 seconds. The teeth were examined by ATR-FTIR spectroscopy and SEM microscopy in order to determine the degree of the enamel demineralization. A major change on teeth composition and morphology has been found. The results are discussed and compared with those of the effects of different concentrations of bleaching agent (carbamide peroxide) on the enamel surface. Etching with 37% ortho-phosphoric acid caused structural and morphological changes to the dental enamel surfaces.
On the Development of a PM2.5 Monitoring Network for Real-time Measurements in Urban Environments
Authors: Savu Tom , Jugravu Bogdan Alexandru , Dunea Daniel
Published: February 2020

A monitoring network system was designed and developed to be deployed in two Romanian cities during the ROkidAIR project for measuring in real time the PM2.5 concentrations. The system comprises 8 stations that were built following the successful design and testing of a PM2.5 optical instrument prototype. The System�s Web services, the data acquisition from the monitoring stations, and the data sending to a GIS-integrated geoportal that includes a decision support system, were firstly evaluated for testing their effectiveness and for eventually defining necessary corrections. After describing the station�s structure and the Web services� main functions, the paper presents data about the most important measured operating parameters of the stations, results from data processing, and conclusions for further developments. Preliminary information collected from a reference gravimetric sampler installed for data inter-comparison with the developed stations is also presented.
Investigation of Heavy Metals Content in Airborne Particles from Ploiesti, Romania
Authors: Iordache Stefania , Dunea Daniel , Radulescu Cristiana , Dulama Ioana Daniela , Ianache Radu , Predescu Mirela
Published: February 2020

This study aims to evaluate the concentrations of heavy metals in airborne particulate matter (PM) collected in Ploiesti city (Romania) during the monitoring campaigns performed in 2015. Various health effects of airborne PM, from less serious to very serious ones, are often associated with its chemical speciation. The heavy metals bounded on PM may accumulate in human body by inhalation and can produce both short-term and long-term negative health effects especially in children and sensitive people. We investigated several heavy metals associated with PM2.5 collected in 12 relevant sampling points to estimate their potential impact on urban population health. PM samples were collected on 37 mm quartz fiberglass filters using a precision optical instrument i.e., DustTrak DRX8533EP. The deposition of elements on the surface of samples was determined using a scanning electron microscope (SEM). The analysis and quantification of trace elements were performed using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). The experiments indicated that samples of PM contained heavy metals in the following order Zn ] Ni ] Cr ] Pb ] As ] Cd. Monitoring of elemental composition in atmospheric particles can contribute to a better air quality management.
The Involvement of Epicardial Adiposity and Inflammation in Postoperatory Atrial Fibrilation - Immunohistochemical Qualitative and Quantitative Assessment
Authors: Butcovan Doina , Oboroceanu Teodor , Cimpeanu Ciprian , Mironescu Aurel , Haliga Raluca Ecaterina , Pinzariu Alin Constantin , Lupusoru Raoul Vasile , Popescu Eugenia , Mocanu Veronica
Published: February 2020

Postoperatory atrial fibrillation (AF) is a common complication after cardiac surgery. Among others cardiac structural changes, epicardial adiposity (EA) and inflammation could be associated with increased cardiovascular risk, including AF. Because there are not enough studies on the association between epicardial fat and inflammation in AF, this paper assesses the association between structural changes in human right atrial appendages (RAA) in patients with and without AF with focus on EA and inflammation. RAA specimens from 20 coronary patients (ages 60 � 10 years) were divided in 2 groups including patients with and without postoperatory AF. Histological, immunohistochemical (IMH) and morphometrical methods were used for assessing EA, myocardial fatty infiltration, epicardial inflammatory foci, cardiomyocytes size and vacuolation and extent of interstitial fibrosis. Atrial changes were found in most patients from both groups, having higher proportions in postoperatory AF patients. EA extent and myocardial fatty infiltration were twofold to threefold higher in patients with postoperatory AF and associated epicardial mononuclear inflammation. RAA structural changes can indicate the susceptibility to develop postoperatory AF. Currently, EA and inflammation have recently emerged as new independent AF risk factors. So, we proposed to study RAA structural changes, including EA, epicardial inflammatory foci in relation with postoperatory AF frequency.
Lugol Iodine Staining Test in Assessing Disease Free Margins in Tonsillar Cancer
Authors: Stefan Catalin , Lostun Gabriel , Lostun Alexandra
Published: February 2020

The carcinoma of the tonsil represents 10-15% of the total of malignant tumors in the oral cavity, having a predilection for males and the great majority is represented by squamous cell carcinomas. In order to improve the long term results of oncologic treatment for patients with tonsillar malignancies early diagnosis and disease free margins during surgery are mandatory. These can be obtained using lugol iodine staining during resection surgery for malignant tumors of the tonsil. This method has been shown to be effective, easy to use, cheap and safe.
Biochemical, Virulence and Resistance Features in Bacterial Strains Recovered from Hospital Surfaces after Decontamination with Quaternary Ammonium Compounds, Triclosan and Iodine Desinfectatnts
Authors: Mitache Mihaela Magdalena , Gheorghe Irina , Totea Georgeta , Bleotu Coralia , Curutiu Carmen , Cochior Daniel , Rusu Elena , Chifiriuc Mariana Carmen
Published: February 2020

The purpose of this study was to investigate the resistance and virulence markers in microbial strains isolated from the hospital environment, recovered after surfaces decontamination with quaternary ammonium compounds, triclosan, iodine disinfectants, in order to predict their role in healthcare-associated infections. The resistance phenotypes were established using disk diffusion and double-disk diffusion test. Simplex and multiplex PCR assays were used to identify the genetic support of cell-associated and soluble virulence factors The Gram-negative bacterial strains isolated from hospital surfaces after decontamination were resistant in high proportions to beta-lactam antibiotics, including penicillins and associations with beta-lactamase inhibitors, third and fourth generation cephalosporins and carbapenems (encoded by blaTEM, blaNDMlike blaCTX-Mlike, blaOXA-48like genes), quinolones (QnrA, gyrB, parE genes), aminoglycosides (aac3Ia gene), and tetracyclines. Regarding the virulence profiles, P. aeruginosa strains revealed the plcH gene (86.6%) followed by plcH, ExoS, algD and ExoU, while the Enterobacteriaceae strains, fimH (24%), followed by papC, sfaDE, hlyA, cnf1, eaea and VT2 genes. S. aureus isolates revealed the SCCmec cassette of type II (40%), followed by type V, IIIA and type IV (2B) and the clfA, clfB, and can adhesion genes. These findings demonstrate that incorrect practices in biocides use, regarding contact time and concentrations or instruments and surfaces insufficient cleaning before disinfection can decrease their effect and favor the persistence of resistant and virulent microbial strains in the hospital environment. The multiple drug resistance and virulence determinants, encoded by diverse genetic elements suggest the potential of these strains to persist and initiate hospital-associated infections.
Microwave Assisted Fischer - Tropsch Synthesis at a Atmospheric Pressure
Authors: Sibianu Ioan Tudor , Berger Daniela , Matei Cristian , Calinescu Ioan
Published: February 2020

The purpose of this study was to test the efficiency of the microwave activation of a Fischer-Tropsch used catalyst under atmospheric pressure. Experiments were carried out on a cobalt, manganese, calcium catalyst on a 10:1:1 molar ratio that was impregnated on a AlSBA-15 support. The amount of metal impregnated was equivalent to 20% of the supports mass. Experiments were carried out both with conventional as well as microwave heating. In order to compare the efficiency of both types of heating, the product compositions were determined at 110, 140, 170, 190, 200, 225, 250 oC. At each temperature 4:1, 2:1, 1.25:1 H2:CO molar ratios were tested. The microwave assisted Fischer-Tropsch reaction allows the use of lower temperatures, as well as larger CO conversion values with better yields especially in methane.
The Evaluation of the C Reactive Protein Levels in the Context of the Periodontal Pathogens Presence in Cardiovascular Risk Patients
Authors: Martu Silvia , Nicolaiciuc Ovidiu , Solomon Sorina , Sufaru Irina , Scutariu Monica , Rezus Ciprian , Popescu Eugenia
Published: February 2020

The purpose of this study was to investigate the serum C-reactive protein (CRP) values in the presence of A. actinomycetemcomitans, P. gingivalis, T. denticola or T. forsythia bacteria, as an indicator of the cardiovascular risk. The study consisted of 64 male and female subjects, aged 55 to 75 years. Subjects were periodontal examined, serum CRP was analyzed, and Porphyromonas gingivalis, A. actinomycetemcomitans, T. forsythia and T. denticola from the subgingival bacterial plaque were detected by real-time quantitative polymerase chain reaction (qPCR). Pathogen prevalence rates were: 45.0% P. gingivalis; 20.5% A. actino-mycetemcomitans; 86.1% T. forsythia; 86.3% T. denticola. The mean CRP was 1.5 (IQR 1.0-2.6) mg / L. There was a significant difference in CRP values between subjects who had P. gingivalis compared to those without (p = 0.003). There were no significant differences for any of the other pathogens. The presence of P. gingivalis was associated with a 1.20-fold increase in CRP. Of the four periodontal pathogens investigated, only the presence of P. gingivalis in subgingival plate samples was significantly associated with a high level of C-reactive protein.
Dystrophic Large Calcification in Breast Cancer
Authors: Ursulescu Corina Lupascu , Ursaru Manuela , Ciobanu Delia , Negru Dragos , Lupascu Cristian
Published: February 2020

There are well established diagnostic criteria for calcifications detected on mammography which allows the division into: benign intermediate concern and higher probability of malignancy according to the morphology. Large calcifications are usually associated with benign breast conditions. We report three cases of breast cancer with large dystrophic calcification on imaging studies (mammography, ultrasound, computed -tomography) and histopatology displayed into the tumor as well as into the tributaries lymph nodes.
Organic Acids Chemical Profiling in Food Items
Authors: Badea Georgiana Ileana , Diaconu Ioana , Radu Gabriel Lucian
Published: February 2020

A fast separation method for simultaneous determination of eleven organic acids was validated and applied to different commercial food items to evaluate their organic acids content. The present method gives detection limits between 0.04 and 4.65 mg mL-1, recovery values in real samples between 78.2 and 97.3% and relative standard deviation values for precision lower than 5%. All validation data were in acceptable range and prove the method�s fit for purpose. The advantages of the method are the short runtime analysis (15 min), no preparation step for the samples before the injection combined with good sensitivity which recommends it for routine control analysis in food industries. Moreover, this methodology has high potential in drinks industry but can by further extended to other types of food items.
Modeling of the Unsteady State Methanol Synthesis at the Level of Catalyst Pellet
Authors: Banu Ionut , Stoica Ioana , Bumbac Gheorghe , Bozga Grigore
Published: February 2020

In a fixed bed of porous catalyst pellets, there are developed two composition and temperature fields, one in the fluid phase and the other one at the level of solid pellets. The objective of this work is to evaluate, by numerical simulation, the unsteady state behavior of a catalyst pellet in typical operating conditions for methanol synthesis reactors. The process kinetics was described by the model published by Graaf et al (1990) and the reaction-diffusion process inside the pellet is based on the Wilke-Bosanquet model. The results showed a short composition and temperature stabilization time, generally below 4 seconds, for a spherical catalyst pellet having typical dimensions for industrial applications.
Synthesis and Structure of a New Class of Fused Heterocycle with 8,9-Dihydro-pyridazino[1,2,4]triazine Skeleton
Authors: Humelnicu Ionel , Vasilache Violeta
Published: February 2020

The synthesis and X-ray crystal structure of a new class of fused heterocycle with 8,9-dihydro-pyridazino[1,2,4]triazine type 2 is reported. The synthesis is facile and efficient and, the structure of compounds was proven by elemental and spectral analysis, the X-ray spectra including (for 2b). The compound crystallizes in the space group P21/N (monoclinic) with a = 9.0077(2) �, b = 10.20019(18) �, c = 14.0099(3) �, a= 90�, b = 89.768(2)�, g= 90�, V= 1287.22(5) and Z = 4. Accurate molecular parameters for the heterocyclic system were obtained from intensity data collected at 200(14) K. The molecule 8,9-dihydro-pyridazino[1,2,4]triazine type 2 is a noncoplanar bicyclic fused system, with an envelope shape onto the triazine moiety, the C12 carbon being out of plane. The methyl group from the C12 carbon it is almost perpendicular on the triazine plane. We also notice a rather powerful hydrogen bond between hydrogen atom from N1 and the oxygen from the C11=O1 carbonyl group.
Experimental Study and Correlation Models of the Density and Viscosity of 2-Methoxy-2-methylpropane with n-propanol
Authors: Budeanu Maria Magdalena , Dumitrescu Vasile
Published: February 2020

Densities (r) and viscosities (h) of the binary systems 2-Methoxy-2-methylpropane with n-propanol were measured at temperatures 288.15, 293.15, 298.15, 303.15 and 308.15 K and atmospheric pressure, over the whole composition range. Belda, Herraez, Emmerling et al. and Gonzalez-Olmos-Iglesias models were used to correlate the densities of binary mixtures. The Mchaweh-Nasrifar-Mashfeghian, Hankinson-Thomson, Yamada-Gunn and Reid et al. models were used to estimate the liquid density of the binary mixtures. Viscosity results were fitted to the equations of Grunberg-Nissan, Heric-Brewer, Jouyban-Acree and McAllister. The thermodynamic activation parameters, free energy, DG�, enthalpy, DH� and entropy, DS� for viscous flow have been estimated and their variations with compositions have been discussed.
Synthesis and Thermal Treatment of Hydroxyapatite Doped with Magnesium, Zinc and Silicon
Authors: Goga Firuta , Forizs Edit , Avram Alexandra , Rotaru Adela , Lucian Anamaria , Petean Ioan , Mocanu Aurora , Cotisel Maria Tomoaia
Published: February 2020

Pure nano-crystalline hydroxyapatite (Hap) and Hap doped with magnesium, zinc and silicon, namely Hap-0.25wt%Mg: Hap01, Hap-0.25wt%Mg-0.47wt%Si: Hap02, Hap-1.50wt%Mg-0.47wt%Si: Hap03, Hap-0.67wt%Mg-0.2wt%Zn-0.13wt%Si: Hap04, were synthesized using aqueous precipitation method. The pure and doped Hap were calcined individually at 400, 650 and 850 �C for 2h, and investigated by Brunauer-Emmett-Teller (BET) specific surface area and porosity measurements, as well as by X-ray powder diffraction (XRD). The morphology and particle size of nano-crystalline powders were investigated using scanning electron microscopy (SEM) and atomic force microscopy (AFM). The thermal stability of the obtained nanoceramics from 30�C to 1000�C and the effect of calcination temperatures (400, 650 and 850 �C) on their composition and structure were also determined by using TG, DTG, TGA and DTA techniques coupled with SEM-EDX. Results analysis shows a high thermal stability (up to 1000 �C) of these nanomaterials, including the triple-substituted Hap with Mg, Zn and Si (Hap04). Simultaneous incorporation of Mg, Zn and Si into Hap lattice represents a novelty and promotes a new generation of synthetic porous nanoceramics with unique Hap structure, and high thermal stability. Due to their chemical composition and structure rather similar to those characteristic for the inorganic component of bone, these nanoceramics can have multiple applications in biomedicine, as bone substitutes, for metal coatings and in drug delivery systems.
Oxidative Stress Markers in Chemically Induced Oral Premalignant Lesions
Authors: Scrobota Ioana , Baciut Grigore , Buzatu Roxana , Szuhanek Camelia , Todor Liana , Vlad Silviu Valentin , Bolfa Pompei , Calniceanu Horia
Published: February 2020

In our study we induced oral premalignant lesions using 4 nitro-quinoline-1-oxide (4NQO) topically applied on oral mucosa for 24 weeks. We detected an oxidative stress marker malondialdehyde (MDA) significantly increased (p[0.05) and an antioxidant/detoxifying activity marker, glutathione (GSH), significantly decreased (p[0.05) in the oral induced epithelial dysplasias. We appreciated that issue MDA and GSH could serve as diagnostic, screening and evaluation of response to therapy tools in oral premalignacy.
Toxic Effects of Magnesium Nitrate on Cardiac Muscle Tissue of Gallus Domesticus Embryos and Chicks
Authors: Teusan Anca , Pinzariu Alin Constantin , Cobzaru Roxana Gabriela , Craus Stefan , Jelihovschi Igor , Crauciuc Dragos Valentin , Crauciuc Eduard Gabriel , Pasca Sorin Aurelian , Lupusoru Raoul Vasile
Published: February 2020

Eggs from avian hybrid ROSS-308 have undergone incubation process, after previously being controlled, sanitized, measured and weighed. We made three groups of eggs, one control (LM) and two experimental (LE1 and LE2).The 3 groups were homogeneous, both as a weight (59.47 to 62.371 g) and volume (55.417 to 58.177 cm3) and surface (59.315 to 72.305 cm2). The eggs of the two experimental groups were injected 0.1 mL of a solution of magnesium nitrate, solution which had a concentration of 0.16%, for LE1 and of 0.61% for LE2. The timing of injection of the solution was at 2, 4, 6 and 8 days after the beginning of the incubation process, for both experimental groups. At the end of incubation period we studied both embryos and the resulting offspring. They were weighed, measured and evaluated in terms of necropsy, and some of them were slaughtered and dissected, extracting from them a series of body organs such as the heart, liver, stomach and intestinal mass. From the heart some histological samples were taken which were processed by paraffin sectioning techniques to yield 20 three-color (HEA) stained histological slides. These samples were studied by optic microscopy and we highlighted the modifications that appeared to the myocardial tissue. Heart weighted between 0.3308 to 0.3843 g, representing 0.807 to 1.062% of the body weight. Myocardial tissue has undergone significant pathological changes such as metaplasia of the vascular wall, perivascular edema, disintegration and degeneration of muscle cells and myocardial steatosis.
Evaluation of Liver Chemistry Tests and Clinical Parameters in Patients with Periodontal Disease and Chronic Hepatitis C
Authors: Gheorghe Dorin Nicolae , Rusu Darian , Herascu Elena , Popescu Dora Maria , Surlin Petra , Rogoveanu Ion
Published: February 2020

The concept of periodontal medicine has been created by taking into consideration the strong connection between the development of the periodontal disease and other general conditions. The presence in blood, saliva and gingival fluid of certain inflammatory markers that are common for the two conditions � periodontitis and chronic hepatitis C, that can generate the appearance of the periodontal inflammation, can be an explication for the probable interconnection of the two conditions. The purpose of this pilot study is to investigate whether chronic hepatitis C can be a worsening factor for the development of the periodontal disease, by setting correlations between the periodontal pathology and some metabolic markers of both hepatitis C and periodontitis patients in comparison to periodontitis-only ones. Positive correlations would justify the expansion of the study for a larger group of patients and the dosage of inflammatory markers for biologic fluids such as blood, saliva and gingival fluid.
Experimental and Modelling Approach of Biogas Production by Anaerobic Digestion of Agricultural Resources
Authors: Dumitrel Gabriela Alina , Cioabla Adrian Eugen , Ionel Ioana , Varga Lucia Ana
Published: February 2020

Anaerobic digestion processes of agricultural resources, as single substrates (wheat bran and barley) or as combination of substrates (75 % corn&25% corn cob � named MIX1 and 40 % corn & 40 % wheat&20 % sunflower husks � named MIX2), were performed, at a mesophilic temperature in a batch reactor, at pilot scale. The results proved that the higher quantity of biogas yield was achieved for barley, followed by MIX1, and finally MIX2. The same order was obtained when the total methane production was evaluated. The performances of digesters were mathematically evaluated by using the modified Gompertz equation. The kinetic parameters, such as the methane production potential (MP), the maximum methane production rate (Rm) and the extent of lag phase (l) were calculated, for each experimental case. The values of the performance indicators confirmed that all the models fitted well with the experimental data.
Study on Toxic Metal Levels in Food Supplements
Authors: Amariei Sonia , Gutt Gheorghe , Oroian Mircea
Published: February 2020

The objective of this study was to evaluate the chronic-toxic risks posed by individual metals via food supplements consumption by computing the hazard quotient (HQ), considering the average daily metal intake ADD (mg/kg�day), daily intake reference dose RfD (mg/kg� day) suggested by the European Food Safety Authority (EFSA), heavy metal concentration C in food supplements (mg/kg), food supplements rate IR (kg/person�day), and the average body weight BW (kg). For this study we took into consideration that the food supplements analyzed are consumed daily at maximum dose. Samples were digested with concentrated nitric and hydrochloric acid and analysed for their inorganic elemental compositions by ICP/MS. The hazard index (HI) was used to estimate total chronic-toxic risks of multiple metals on the assumption of dose additivity. Cd hazard index is over one and it can be considered a great issue due to its chemical toxicology. The Cd hazard index is much greater in the case of animal and mineral food supplements than vegetal supplements. In the case of the other heavy metals analyzed (Cr, Cu, Zn, As, Hg and Pb) the hazard index is very low so their toxicological potential is negligible.
Effect of Process Factors on Pervaporation Dehydration of Isopropanol
Authors: Janabi Ali A. A. Al , Parvulescu Oana Cristina , Trica Bogdan , Dobre Tanase
Published: February 2020

The paper aimed at studying the performances of pervaporation separation of isopropanol-water system using a Pervatech ceramic membrane at various values of feed mixture flow rate (F=1000 kg/hr), feed water mass fraction (xF=0.1-0.2), operation temperature (t=60-90 �C), permeate pressure (pP=1000-9000 Pa) and water separation degree (sW=0.9, 0.95). Membrane total flux and separation factor were predicted applying a second order response surface model with 3 factors, i.e., xF, t and pP. An algorithm for estimating the membrane surface area was presented. Membrane area increased with sW and xF and its lowest values (A=13 m2 for xF=0.1 and A=24 m2 for xF=0.2) were attained for t=60 �C and pP=9000 Pa. These findings could be applied for optimizing the process of isopropanol dehydration by pervaporation.
A Comparative Three- and Bi-dimensional Research of the Marginal Fit of Pressed Lithium Disilicate Inlays
Authors: Baciu Sorana , Berece Cristian , Florea Adrian , Burde Alexandru Victor , Negrutiu Meda Lavinia , Szuhanek Camelia , Sinescu Cosmin , Duma Virgil Florin , Manole Marius
Published: February 2020

The replacement of classical fillings, especially in the posterior area with different types of inlays has become a common solution used in order to improve all parameters which insure a long lasting treatment. Vrious ceramic materials and systems are available, but so far an ideal ceramic material suited for all clinical situations, has not been found. The range of ceramic materials as: lithium disilicate, aluminum oxide, zirconium oxide and hybrid ceramics enhance the possibilities of using ceramic inalys in various clinical situations. Marginal adaptation is one of the most important conditions for long-term success for any type of material. The marginal discrepancy of fixed restorations is one of the main factors which lead to failure of the prosthetic treatment.The purpose of the present study is to compare a 2D and 3D method for evaluating the marginal fit for pressed lithium dislicate inlays (Emax). The two used methods were: SEM- electronic microscopy and micro CT.
The Evaluation of the Influence of Geographic and Meteorological Factors on Heat Transfer in the Case of Crude Oil Storage in Overground Tanks
Authors: Cristescu Tudora , Stoica Monica Emanuela , Suditu Silvian
Published: February 2020

The study aims to analyze the main factors that influence the transfer of heat in the case of crude oil storage. A model based on the computing relations taken from specific publications was developed. The case studies were conducted on the basis of experimental research on several oil storage tanks, located in an oil transit station in Romania. The following two cases were analyzed, i.e., when the crude oil is heated and stagnates in the storage tank, and when it only stagnates, respectively. The analysis and application of the developed standard model facilitated the establishing of the factors that influence heat transfer. The influence of the geographic position and meteorological factors was also analyzed, which led to the formulation of conclusions with respect to the heat loss that occurs through the walls of the tanks.