bluuebunny/crossref_metadata_2025 huggingface dataset:
Single-Center Experience With Endovascular Treatment for Splenic Artery Aneurysms in Long-Term Follow-Up: A Retrospective Study
Authors: Cao Yanyan , Song Songlin , Ouyang Tao , Zheng Chuansheng
Published: January 2022

Objectives

To reveal a single-center experience with endovascular treatment for splenic artery aneurysm (SAA) and analyze the safety and efficacy of the operation in the long-term follow-up.

Materials and Methods

A total of 49 patients with SAAs (21 men, 28 women; mean age, 52.4 ± 11.5 years) were enrolled in this study from July 2010 to December 2020. Baseline and characteristics of SAAs were collected. Parent artery coil embolization or combined with sac coil embolization of SAAs, graft-stent implantation, or bare-stent-assisted coil embolization were performed for the treatment of SAAs. Adverse events and follow-up data were recorded.

Results

The average diameter of SAAs was 3.3 ± 2.5 cm (range, 1.0–13.6 cm). An individual-tailed modality was conducted for three patients. A 100% technical success rate was achieved. No re-intervention procedure was performed in all patients. No major treatment-related adverse events were observed, and no expansion or rupture of SAAs occurred in the average follow-up period of 57.9 ± 27.3 months (19–125 months).

Conclusion

Endovascular treatment of SAA, including the individual-tailed therapy for three cases, is safe, effective, and minimally invasive with high technical success rates and satisfactory outcomes during the long-term follow-up period.
Study of Resilience in Learning Environments During the Covid-19 Pandemic
Authors: Raghunathan Shriram , Darshan Singh Abtar , Sharma Bibhya
Published: January 2022

The Covid-19 pandemic has caused a great change in the world. One aspect of the pandemic is its effect on Educational systems. Educators have had to shift to a pure online based system. This shift has been sudden and without any prior warning. Despite this the Educational system has survived and exhibited resilience. The resilience of a system can be determined if the system continues to operate or function as effectively as before a change. Resilience in a system implies the ability to work and develop when the forces in the environment are unexpected, abrupt and sudden as well. The environment may change or evolve but the underlying system must keep functioning, developing and responding. Resilience is a trait in a system. It is a set of characteristics in the system that enables it to sustain itself in the face of change. A resilient system can cope and prosper in the face of change. For the domain of education, the Covid-19 pandemic served as a phenomenal change event and a wakeup call to the education fraternity. As a social system, resilience meant that the people in the educational environment continued to function albeit differently. The environment, meaning the processes, hierarchy and the intricate social ties in the system contributed to the resiliency of the system. Thus the measure of resilience in education has three major facets—people, the technology which facilitates the process and the process environment. This work aims to understand the resilience of the teachers due to the Covid-19 pandemic, especially how learning continued and what contributed to this continuity. Resilience research and understanding is as important as the pedagogical and technological aspects in an Educational system as it is a trait that encompasses the people, the socio-economic system and their relationships. In this work, we analyzed resilience as trait, its relevance in an Educational system, factors that make up resilience in an Educational system and finally the relevant research about resilience in Education during Covid-19. Based on the results of our literature review we formed a model for Educators. A survey was conducted among educators of three countries namely Malaysia, Fiji, and India to determine the essential elements of resilience that were relevant to the continuity of an educational system from the point of view of teachers. We arrived at a set of factors that are relevant to the teachers in the educational systems which can be an impetus for policy makers to focus on and develop. The major results from the study are the need for Educational systems to focus on three facets—internal, interpersonal and external aspects of teachers and strengthen factors such as support for teachers, strong academic leadership, trust of teachers, increase self-motivation, enhance communication with stakeholders and emphasize systems that enhance student-teacher communication. The future areas of research are also discussed in the work.
The International Spillover Effect of Import Trade on Energy Efficiency in the Post-COVID-19 Era: Evidence From China
Authors: Hao Xiaoli , Wang Xinhui , Xue Yan
Published: January 2022

China is in a transition period—its government has been expanding imports and pushing itself to shift from a world factory to a world market. One of the aims is to promote energy reform and ensure energy security. Taking the resource-based regions of China as objects, based on 2003–2017 panel data, this paper investigates energy efficiency loss by the stochastic frontier approach and the effects of different technical inefficiency items. Surprisingly, quantitative results show that 99.9% energy efficiency loss in these regions is caused by technical inefficiency (which had never been found and discussed in previous studies). However, this does not mean that China’s efforts to expand imports as a way to improve energy efficiency and energy security are undesirable. Instead, interestingly, it is import (−0.083***) rather than industrial structure (0.524***) that can significantly reduce energy efficiency loss. Then, it employs the counter-fact test to quantify the positive accelerating effect of human capital (average as high as 4.1%) as a key factor of absorptive capacity in the technology spillover. Lastly, it puts forward the corresponding policy suggestions in energy fields, to solve the problem effectively, especially the “comprehensive technology spreading center” and “innovative three-dimensional talent supplementary and flow mechanism.”
Research on the Peak Carbon Dioxide Emission Strategy of Chinese Port Based on Carbon Emission Estimation
Authors: Fan Shenghai , Lu Ziai
Published: January 2022

Ports are an important node of a country’s external goods circulation, as well as large consumers of energy consumption. This paper uses the STIRPAT (Stochastic Impacts by Regression on Population, Affluence, and Technology) model to study the trend of carbon emissions under different scenarios based on the energy consumption data of Chinese ports from 2010 to 2019 and analyzes the possibility of the peak carbon dioxide emission of Chinese ports. The results show that the carbon emissions of Chinese ports have peaked in 2013 under most scenarios, with CO2 emission of 9,213,500 tons and carbon emission intensity of 0.783 tons of CO2/103-tons of throughput. In addition, considering other scenarios, carbon emission action strategies should be formulated according to the differences of carbon emission peaking conditions of specific ports.
Community Knowledge and Concerns About Urban Soil Science, Practice, and Process: Perspectives From the Healthy Soils for Healthy Communities Initiative in Los Angeles, CA, United States
Authors: Schwarz Kirsten , Wohldmann Erica L. , Chen Yujuan , Pouyat Richard V. , Gonzalez Andres , Mao Selena , Day Susan D.
Published: January 2022

Urban soil systems research has largely relied on the narrative that soils provide ecosystem services to human populations and should be studied and managed to maximize their potential value in regards to such services. However, soil scientists have not adequately engaged with diverse stakeholders to understand the needs, opportunities, and challenges related to urban soil systems. This disconnect has resulted in urban soil system research agendas that are potentially misaligned with the needs of the communities in which they are situated. Community engagement in the priorities-setting stage of research development can create research agendas that more closely align with community needs. Here we report on findings from the Healthy Soils for Healthy Communities Initiative in which community perceptions, needs, and concerns regarding soils in Los Angeles (LA) County, California, United States were measured through four county-wide online surveys with residents, educators, policy-makers, and professionals and a series of virtual focus groups with key community representatives. The online surveys revealed that the majority of LA County residents (76%) are very or extremely concerned about soil contaminants and pollution. Likewise, 70% of policy-makers and 77% of LA County professionals are highly concerned about soil contamination. In contrast, fewer LA County educators (48%) are concerned about soil contaminants and pollution. Even though 85% of LA County residents surveyed maintain some kind of green space, 70% self-report that their knowledge regarding factors impacting soil health is low. The focus groups revealed several themes present across stakeholder groups including a need for: (1) accessible and transparent soil data and testing; (2) effective community engagement and streamlined communication that centers underserved communities; and (3) building alliances among community, policy, businesses, and science professionals and leveraging connections among organizations, individuals, and agencies that are focused on soil. The findings from this study have informed the future direction of urban soil research and community science in the region. The process of engaging communities in defining research agendas can serve as a model for other cities providing an opportunity to not only improve the relevance and impact of urban soil research, but also improve the sustainability of urban soil systems.
Development and Validation of Genome Instability-Associated lncRNAs to Predict Prognosis and Immunotherapy of Patients With Hepatocellular Carcinoma
Authors: Yan Yifeng , Ren Liang , Liu Yan , Liu Liang
Published: January 2022

The pathophysiology of hepatocellular carcinoma (HCC) is prevalently related to genomic instability. However, research on the association of extensive genome instability lncRNA (GILnc) with the prognosis and immunotherapy of HCC remains scarce. We placed the top 25% of somatic mutations into the genetically unstable group and placed the bottom 25% of somatic mutations into the genetically stable group, and then to identify different expression of GILnc between the two groups. Then, LASSO was used to identify the most powerful prognostic GILnc, and a risk score for each patient was calculated according to the formula. Based on a computational frame, 245 different GILncs in HCC were identified. An eight GILnc model was successfully established to predict overall survival in HCC patients based on LASSO, then we divided HCC patients into high-risk and low-risk groups, and a significantly shorter overall survival in the high-risk group was observed compared to those in the low-risk group, and this was validated in GSE76427 and Tongji cohorts. GSEA revealed that the high-risk group was more likely to be enriched in cancer-specific pathways. Besides, the GILnc signature has greater prognostic significance than TP53 mutation status alone, and it is capable of identifying intermediate subtype groups existing with partial TP53 functionality in TP53 wild-type patients. Importantly, the high-risk group was associated with the therapeutic efficacy of PD-L1 blockade, suggesting that the development of potential drugs targeting these GILnc could aid the clinical benefits of immunotherapy. Finally, the GILnc signature model is better than the prediction performance of two recently published lncRNA signatures. In summary, we applied bioinformatics approaches to suggest that an eight GILnc model could serve as prognostic biomarkers to provide a novel direction to explore the pathogenesis of HCC.
A Simple and Efficient Method to Cryopreserve Human Ejaculated and Testicular Spermatozoa in −80°C Freezer
Authors: Wang Xiaohan , Lu Fangting , Bai Shun , Wu Limin , Huang Lingli , Zhou Naru , Xu Bo , Wan Yangyang , Jin Rentao , Jiang Xiaohua , Tong Xianhong
Published: January 2022

Human autologous sperm freezing involves ejaculated sperm, and testicular or epididymal puncture sperm freezing, and autologous sperm freezing is widely used in assisted reproductive technology. In previous studies, researchers have tried to cryopreserve sperm from mammals (rats, dogs, etc.) using a −80°C freezer and have achieved success. It is common to use liquid nitrogen vapor rapid freezing to cryopreserve human autologous sperm. However, the operation of this cooling method is complicated, and the temperature drop is unstable. In this study, we compared the quality of human ejaculation and testicular sperm after liquid nitrogen vapor rapid freezing and −80°C freezing for the first time. By analyzing sperm quality parameters of 93 ejaculated sperm and 10 testicular sperm after liquid nitrogen vapor rapid freezing and −80°C freezing, we found reactive oxygen species (ROS) of sperm of the −80°C freezer was significantly lower than liquid nitrogen vapor rapid freezing. Regression analysis showed that progressive motility, ROS, and DNA fragmentation index (DFI) in post-thaw spermatozoa were correlated with sperm progressive motility, ROS, and DFI before freezing. For the freezing method, the −80°C freezer was positively correlated with the sperm progressive motility. Among the factors of freezing time, long-term freezing was negatively correlated with sperm progressive motility and ROS. Although freezing directly at −80°C freezer had a slower temperature drop than liquid nitrogen vapor rapid freezing over the same period, the curves of the temperature drop were similar, and slight differences in the freezing point were observed. Furthermore, there were no statistically significant differences between the two methods for freezing testicular sperm. The method of direct −80°C freezing could be considered a simplified alternative to vapor freezing for short-term human sperm storage. It could be used for cryopreservation of autologous sperm (especially testicular sperm) by in vitro fertilization centers.

Clinical Trial Registration: (website), identifier (ChiCTR2100050190).
SARS-CoV-2 Infection Triggers Auto-Immune Response in ARDS
Authors: Juanes-Velasco Pablo , Landeira-Viñuela Alicia , García-Vaquero Marina L. , Lecrevisse Quentin , Herrero Raquel , Ferruelo Antonio , Góngora Rafael , Corrales Fernando , Rivas Javier De Las , Lorente Jose A. , Hernández Ángela-Patricia , Fuentes Manuel
Published: January 2022

Acute respiratory distress syndrome (ARDS) is a severe pulmonary disease, which is one of the major complications in COVID-19 patients. Dysregulation of the immune system and imbalances in cytokine release and immune cell activation are involved in SARS-CoV-2 infection. Here, the inflammatory, antigen, and auto-immune profile of patients presenting COVID-19-associated severe ARDS has been analyzed using functional proteomics approaches. Both, innate and humoral responses have been characterized through acute-phase protein network and auto-antibody signature. Severity and sepsis by SARS-CoV-2 emerged to be correlated with auto-immune profiles of patients and define their clinical progression, which could provide novel perspectives in therapeutics development and biomarkers of COVID-19 patients. Humoral response in COVID-19 patients’ profile separates with significant differences patients with or without ARDS. Furthermore, we found that this profile can be correlated with COVID-19 severity and results more common in elderly patients.
Three Specific Potential Epitopes That Could Be Recognized by T Cells of Convalescent COVID-19 Patients Were Identified From Spike Protein
Authors: Zhang Yunwen , Yang Zhengrong , Tang Mingyuan , Li Hao , Tang Tian , Li Guilian , Zhong Yifan , Zhang Xiaomin , Wang Xiaohui , Wang Chuan
Published: January 2022

The current coronavirus disease 2019 (COVID-19) vaccines are used to prevent viral infection by inducing neutralizing antibody in the body, but according to the existing experience of severe acute respiratory syndrome coronavirus (SARS) infection, T-cell immunity could provide a longer durable protection period than antibody. The research on SARS-CoV-2-specific T-cell epitope can provide target antigen for the development and evaluation of COVID-19 vaccines, which is conducive to obtain COVID-19 vaccine that can provide long-term protection. For screening specific T-cell epitopes, a SARS-CoV-2 S protein peptide library with a peptide length of 15 amino acids was synthesized. Through flow cytometry to detect percentage of IFN-γ+ T cells after mixed COVID-19 convalescent patients’ peripheral blood mononuclear cell with peptide library, seven peptides (P77, P14, P24, P38, P48, P74, and P84) that can be recognized by the T cells of COVID-19 convalescent patients were found. After excluding the nonspecific cross-reactions with unexposed population, three SARS-CoV-2-specific T-cell potential epitopes (P38, P48, and P84) were finally screened with the positive reaction rates between 15.4% and 48.0% in COVID-19 convalescent patients. This study also provided the HLA allele information of peptide-positive-response COVID-19 convalescent patients, thus predicting the population coverage of these three potential epitopes. Some HLA alleles showed higher frequency of occurrence in COVID-19 patients than in total Chinese population but no HLA alleles related to the T-cell peptide response and the severity of COVID-19. This research provides three potential T-cell epitopes that are helpful for the design and efficacy evaluation of COVID-19 vaccines. The HLA information provided by this research supplies reference significance for subsequent research such as finding the relation of HLA genotype with disease susceptibility.
‘Bouncing Back’ From Subclinical Malaria: Inflammation and Erythrocytosis After Resolution of P. falciparum Infection in Gambian Children
Authors: Mooney Jason P. , DonVito Sophia M. , Jahateh Maimuna , Bittaye Haddy , Keith Marianne , Galloway Lauren J. , Ndow Mortala , Cunnington Aubrey J. , D’Alessandro Umberto , Bottomley Christian , Riley Eleanor M.
Published: January 2022

Recent malaria is associated with an increased risk of systemic bacterial infection. The aetiology of this association is unclear but malaria-related haemolysis may be one contributory factor. To characterise the physiological consequences of persistent and recently resolved malaria infections and associated haemolysis, 1650 healthy Gambian children aged 8–15 years were screened for P. falciparum infection (by 18sRNA PCR) and/or anaemia (by haematocrit) at the end of the annual malaria transmission season (t1). P. falciparum-infected children and children with moderate or severe anaemia (haemoglobin concentration < 11g/dl) were age matched to healthy, uninfected, non-anaemic controls and screened again 2 months later (t2). Persistently infected children (PCR positive at t1 and t2) had stable parasite burdens and did not differ significantly haematologically or in terms of proinflammatory markers from healthy, uninfected children. However, among persistently infected children, IL-10 concentrations were positively correlated with parasite density suggesting a tolerogenic response to persistent infection. By contrast, children who naturally resolved their infections (positive at t1 and negative at t2) exhibited mild erythrocytosis and concentrations of pro-inflammatory markers were raised compared to other groups of children. These findings shed light on a ‘resetting’ and potential overshoot of the homeostatic haematological response following resolution of malaria infection. Interestingly, the majority of parameters tested were highly heterogeneous in uninfected children, suggesting that some may be harbouring cryptic malaria or other infections.
Childhood-Onset Movement Disorders Can Mask a Primary Immunodeficiency: 6 Cases of Classical Ataxia-Telangiectasia and Variant Forms
Authors: Blanchard-Rohner Geraldine , Peirolo Anna , Coulon Ludivine , Korff Christian , Horvath Judit , Burkhard Pierre R. , Gumy-Pause Fabienne , Ranza Emmanuelle , Jandus Peter , Dibra Harpreet , Taylor Alexander Malcolm R. , Fluss Joel
Published: January 2022

Ataxia-telangiectasia (A-T) is a neurodegenerative and primary immunodeficiency disorder (PID) characterized by cerebellar ataxia, oculocutaneous telangiectasia, immunodeficiency, progressive respiratory failure, and an increased risk of malignancies. It demands specialized care tailored to the individual patient’s needs. Besides the classical ataxia-telangiectasia (classical A-T) phenotype, a variant phenotype (variant A-T) exists with partly overlapping but some distinctive disease characteristics. Here we present a case series of 6 patients with classical A-T and variant A-T, which illustrates the phenotypic variability of A-T that can present in childhood with prominent extrapyramidal features, with or without cerebellar ataxia. We report the clinical data, together with a detailed genotype description, immunological analyses, and related expression of the ATM protein. We show that the presence of some residual ATM kinase activity leads to the clinical phenotype variant A-T that differs from the classical A-T. Our data illustrate that the diagnosis of the variant form of A-T can be delayed and difficult, while early recognition of the variant form as well as the classical A-T is a prerequisite for providing a correct prognosis and appropriate rehabilitation and support, including the avoidance of diagnostic X-ray procedures, given the increased risk of malignancies and the higher risk for side effects of subsequent cancer treatment.
Autoantibody:Autoantigen Competitor Decoys: Application to Cardiac Phenotypes
Authors: Cardozo Timothy , Cardozo Lila , Boutjdir Mohamed
Published: January 2022

Autoimmune diseases are often associated with autoantibodies that abnormally target self-antigens (autoantigens). An intuitive therapeutic strategy for diseases caused by aAbs is to design decoys, or soluble molecules that target the antigen combining site of these aAbs, thereby blocking binding of aAb to self-antigen and subsequent tissue damage. Here, we review the known decoy molecules of these types, discuss newer technological opportunities afforded by monoclonal antibody and structural biology advances, and discuss the challenges to this approach. Recent opportunities relevant to this approach for cardiac phenotypes, specifically Ro-associated long QT syndrome, are discussed.
P311 Facilitates the Angiogenesis and Wound Healing Function of MSCs by Increasing VEGF Production
Authors: Liu Zhihui , Yang Jiacai , Chen Yunxia , Chen Cheng , Wang Jue , Lee Yew Mun , Zheng Wenxia , Shang Ruoyu , Tang Yuanyang , Zhang Xiaorong , Hu Xiaohong , Huang Yong , Peng Shiya , Liou Yih-Cherng , He Weifeng , Luo Gaoxing
Published: January 2022

As a potential clinical therapeutic cell for injured tissue repair, mesenchymal stem cells (MSCs) have attracted increasing attention. Enhancing the pro-healing function of MSCs has gradually become an essential topic in improving the clinical efficacy of MSCs. Recently, studies have shown that neuronal protein 3.1 (P311) plays a crucial role in promoting skin wound healing, suggesting P311 gene modification may improve the pro-healing function of MSCs. In this study, we demonstrated that increasing the in vivo expression of P311 could significantly enhance the ability of MSCs to lessen the number of inflammatory cells, increase the expression of IL10, reduce the levels of TNF-α and IFN-γ, increase collagen deposition, promote angiogenesis, and ultimately accelerate skin wound closure and improve the quality of wound healing. Importantly, we uncovered that P311 enhanced the pro-angiogenesis function of MSCs by increasing the production of vascular endothelial growth factor (VEGF) in vitro and in vivo. Mechanistically, we revealed that the mTOR signalling pathway was closely related to the regulation of P311 on VEGF production in MSCs. Together, our data displayed that P311 gene modification in MSCs augments their capabilities to promote skin wound closure, which might bring the dawn for its clinical application in the future.
C57BL/6 Mice Pretreated With Alpha-Tocopherol Show a Better Outcome of Trypanosoma cruzi Infection With Less Tissue Inflammation and Fibrosis
Authors: Silva Amanda C. O. , Bonfim Maiara , Fontes Jonathan L. M. , dos-Santos Washington L. C. , Mengel José , Cardillo Fabíola
Published: January 2022

Chagas disease is accompanied by a multisystem inflammatory disorder that followsTrypanosoma cruziinfection. Alpha-tocopherol has been described as an antioxidant and a potential adjuvant to enhance immune responses to vaccines. Therefore, we have evaluated the immune response toT. cruziinfection upon alpha-tocopherol pre-administration. The results show that administration of alpha-tocopherol before the infection results in lower parasitemia and lower mortality of C57BL/6 mice infected with theTulahuen T. cruzistrain. Alpha-tocopherol administration in normal C57BL/6 mice resulted in higher levels of IFN-γ production by T and NK cells before and after the infection withT. cruzi. More importantly, previous administration of alpha-tocopherol increased the production of IL-10 by T and myeloid suppressor cells and the formation of effector memory T cells while decreasing the expression of PD-1 on T cells. These results suggest that alpha-tocopherol may limit the appearance of dysfunctional T cells during the acute and early chronic phases ofT. cruziinfection, contributing to control infection. In addition, alpha-tocopherol could diminish tissue inflammation and fibrosis in late acute disease. These results strongly suggest that alpha-tocopherol may be a helpful agent to be considered in Chagas disease.
Public Perceptions of Marine Plastic Litter: A Comparative Study Across European Countries and Seas
Authors: van Oosterhout Lotte , Dijkstra Hanna , van Beukering Pieter , Rehdanz Katrin , Khedr Salma , Brouwer Roy , Duijndam Sem
Published: January 2022

Marine plastic litter (MPL) is a growing global problem and its prevention requires public engagement and behavioral change. Statistics of public perceptions of MPL are scarce and hardly comparable due to varying definitions and interpretations of the concept. This study identifies and classifies relevant components of public perceptions of MPL based on a large-scale survey across eight European countries sharing three European seas (North Sea, Baltic Sea, and the Mediterranean Sea). High levels of concern about MPL were observed throughout the EU and water pollution and plastics in oceans were consistently ranked in the top four most worrisome environmental challenges of our time. Most of the respondents (70%) reported noticing MPL, which influenced knowledge and feelings of responsibility with regards to MPL. The general public held companies and consumers most responsible for cleaning up MPL. Self-responsibility to reduce MPL varies considerably across and within countries, with the highest scores being reported in Greece and the lowest in Netherlands. Public knowledge on the recyclability of plastics was low in all countries. At the marine region level, the lowest scores for concern, perceived consequences and personal responsibility to reduce the use of plastics were reported in the North Sea region, followed by the Baltic Sea region and the highest scores were recorded in the Mediterranean Sea region. Using these results, policy implications and possible intervention strategies are discussed, to improve and increase public awareness, understanding, engagement, and sense of responsibility to change lifestyles and purchasing behavior to prevent and reduce MPL.
The Value of Nasal and Oral Clinical Examination in Febrile Neutropenic Patients for Initiating Antifungal Therapy as a Preemptive Method
Authors: Salehi Mohammadreza , Ghaderkhani Sara , Sharifian Ramezan Ali , Dehghan Manshadi Seyed Ali , Samiee Fard Elahe , Khodavaisy Sadegh , Pourahmad Ramtin , Foroushani Abbas Rahimi , Rodini Kamran , Kamali Sarvestani Hasti
Published: January 2022

Background

Invasive fungal infections (IFIs) are complications that lead to mortality and morbidity in hematologic malignancies. The time of starting antifungal therapy is vital. Preemptive antifungal therapy has appeared recently as a new policy for the management of IFIs based on noninvasive ways in neutropenic patients.

Methods

We enrolled leukemia patients with neutropenia after chemotherapy in Imam Khomeini Hospital Complex, Tehran, Iran. Patients who entered the neutropenic phase were divided into two categories (empirical and preemptive) for receiving antifungal agents. The patients were clinically examined in the preemptive group every day to find IFIs. As soon as clinical evidence of IFIs was observed, antifungal was prescribed. The empirical group patients received antifungals based on the ward protocol. Based on the data in each group, the diagnostic and therapeutic results of cases are followed-up to 3 months. To compare percentages between the two groups, the chi-squared test was used. And to compare two means between the two groups, the independent t-test was used. All the statistical analyses were done in the Statistical Package for the Social Sciences (SPSS) version 24 software (IBM Corporation, Armonk, New York, USA).

Results

We assessed 132 leukemic patients with inclusion and exclusion criteria. Eventually, 80 patients were enrolled. The mean age was 35.52 years. Demographics data and distribution of leukemia type show no significant differences between the two groups. Despite a higher percentage of IFIs discovered in the preemptive group than the empirical group (25 vs. 18.75%, respectively), but data show no significant differences. The average days of IFIs diagnosis since the beginning of neutropenia in the empirical group were 9.5 days while in the preemptive group, the average days were 5.4 days (p < 0.05). Totally, there were 15 patients with a proven IFI in each group (40% in the empirical group and 60% in the preemptive group). Results significantly show an increase in surgical sinus debridement in the empirical groups (83.3%) vs. the preemptive groups (55.5%), (p < 0.05). The mortality rate differed significantly among the two groups; it was 7.5% in the preemptive group and 25% in the empirical group (p < 0.05).

Conclusion

Daily oral and nasal cavities examination to find the symptoms of IFIs and then start preemptive antifungal agents may be able to lead to accurate diagnosis, earlier treatment, and decreasing sinus surgery debridement in leukemia patients with neutropenia.
Diagnostic Accuracy of H. pylori Status by Conventional Endoscopy: Time-Trend Change After Eradication and Impact of Endoscopic Image Quality
Authors: Quach Duc Trong , Aoki Rika , Iga Akiko , Le Quang Dinh , Kawamura Toru , Yamashita Ken , Tanaka Shinji , Yoshihara Masaharu , Hiyama Toru
Published: January 2022

Aim

To assess the time trend of diagnostic accuracy of pre- and post-eradication H. pylori status and interobserver agreement of gastric atrophy grading.

Methods

A series 100 of conventional endoscopic image sets taken from subjects undergoing gastric cancer screening at a polyclinic were evaluated by 5 experienced assessors. Each assessor independently examined endoscopic findings according to the Kyoto classification and then determined the H. pylori status (never, current, or past infected). Gastric atrophy was assessed according to the Kimura-Takemoto classification and classified into 3 grades (none/mild, moderate, or severe). The image series that ≥3 assessors considered to have good quality were arbitrarily defined as high-quality image (HQI) series, and the rest were defined as low-quality image (LQI) series.

Results

The overall diagnostic accuracy of H. pylori status was 83.0%. It was lowest in subjects with current infection (54%), gradually increased at 1 year (79%, P < 0.001) and 3 years (94.0%, P = 0.002), but then did not significantly change at 5 years (91.0%, P = 0.420) after eradication. The rate of LQI series was 28%. The overall diagnostic accuracy of H. pylori status dropped from 88.9% to 67.9% (P < 0.001), and the mean kappa value on gastric atrophy grading dropped from 0.730 to 0.580 (P = 0.002) in the HQI and LQI series, respectively.

Conclusions

Diagnostic accuracy of H. pylori status increased over time after eradication. LQI series badly affected the diagnostic accuracy of H. pylori status and the level of agreement when grading gastric atrophy.
The Cpx Stress Response Regulates Turnover of Respiratory Chain Proteins at the Inner Membrane of Escherichia coli
Authors: Tsviklist Valeria , Guest Randi L. , Raivio Tracy L.
Published: January 2022

The Cpx envelope stress response is a major signaling pathway monitoring bacterial envelope integrity, activated both internally by excessive synthesis of membrane proteins and externally by a variety of environmental cues. The Cpx regulon is enriched with genes coding for protein folding and degrading factors, virulence determinants, and large envelope-localized complexes. Transcriptional repression of the two electron transport chain complexes, NADH dehydrogenase I and cytochrome bo3, by the Cpx pathway has been demonstrated, however, there is evidence that additional regulatory mechanisms exist. In this study, we examine the interaction between Cpx-regulated protein folding and degrading factors and the respiratory complexes NADH dehydrogenase I and succinate dehydrogenase in Escherichia coli. Here we show that the cellular need for Cpx-mediated stress adaptation increases when respiratory complexes are more prevalent or active, which is demonstrated by the growth defect of Cpx-deficient strains on media that requires a functional electron transport chain. Interestingly, deletion of several Cpx-regulated proteolytic factors and chaperones results in similar growth-deficient phenotypes. Furthermore, we find that the stability of the NADH dehydrogenase I protein complex is lower in cells with a functional Cpx response, while in its absence, protein turnover is impaired. Finally, we demonstrated that the succinate dehydrogenase complex has reduced activity in E. coli lacking the Cpx pathway. Our results suggest that the Cpx two-component system serves as a sentry of inner membrane protein biogenesis, ensuring the function of large envelope protein complexes and maintaining the cellular energy status of the cell.
The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2
Authors: Liu Jian , Liu Kaiquan , Zhao Zhe , Wang Zheng , Wang Fengchao , Xin Yuxiu , Qu Jie , Song Feng , Li Zhenghua
Published: January 2022

Cyanobacterial blooming is an increasing environmental issue all over the world. Algicidal bacteria are potential tools for the control of algal blooms. The algicidal activity in many bacteria exhibits quorum-sensing (QS) dynamics and the regulatory mechanism of this activity in these bacteria is unclear. In this study, combining genomic sequencing and genome editing, we have identified that the primary quorum-sensing system in the isolated algicidal strain Shewanella xiamenensis Lzh-2 is the LuxS/AI-2 signaling pathway. Disruption of the QS system through recombination deletion of the LuxS gene led to a loss of algicides production and algicidal activity. Restoration of the LuxS gene in the deletion mutant compensated the QS system and recovered the algicidal activity. Consequently, we proved that Lzh-2 regulates the algicidal activity through LuxS/AI-2 quorum-sensing system.
Rapid and Specific Detection of Active SARS-CoV-2 With CRISPR/Cas12a
Authors: Liu Xinyi , Li Yanhua , Wang Xin , Song Yifan , Wu Lina , Yu Benyuan , Ma Xiaodong , Ma Peixiang , Liu Ming , Huang Xingxu , Wang Xinjie
Published: January 2022

Rapid and sensitive nucleic acid detection of SARS-CoV-2 has contributed to the clinical diagnosis and control of COVID-19. Although detection of virus genomic RNA (gRNA) has been commonly used in clinical diagnosis, SARS-CoV-2 gRNA detection could not discriminate between active infectious virus with remnant viral RNA. In contrast to genomic RNA, subgenomic RNAs (sgRNAs) are only produced when the virus is actively replicating and transcription, detection of sgRNA could be an indication to evaluate infectivity. CRISPR/Cas-based nucleic acid detection methods have been considered potential diagnostic tools due to their intrinsic sensitivity, specificity and simplicity. In this study, to specifically detect active virus replication, we developed a CRISPR-based active SARS-CoV-2 (CRISPR-actCoV) detection strategy by detecting sgRNAs of SARS-CoV-2. CRISPR-actCoV with CRISPR Cas12a-assisted fluorescence reporter system enables detection of sgRNAs at 10 copies in 35 min with high specificity and can be read out with naked eyes. Further, we performed CRISPR-actCoV mediated sgRNA detection in 30 SARS-CoV-2 potentially infected clinical samples, and 21 samples were SARS-CoV-2 sgRNA positive. A quantitative RT-PCR assay was also performed to detect gRNA of SARS-CoV-2 in parallel. Among the 30 clinical samples, 27 samples were gRNA positive. Taken together, CRISPR-actCoV provides an alternative for rapid and accurate detection of active SARS-CoV-2 and has great significance in better response of coronavirus causing epidemic disease.
Aspirin Repurposing in Folate-Decorated Nanoparticles: Another Way to Target Breast Cancer
Authors: Kanwal Fariha , Ma Mingming , Rehman Muhammad Fayyaz ur , Khan Fahim-ullah , Elizur Shai E. , Batool Aima Iram , Wang Chi Chiu , Tabassum Tahira , Lu Changrui , Wang Yao
Published: January 2022

Breast cancer affects more than 1 million women per year worldwide. Through this study, we developed a nanoparticle-based drug delivery system to target breast cancer cells. Aspirin has been found to inhibit thromboembolic diseases with its tumor-preventing activity. As a consequence, it relieves disease symptoms and severity. Here, mesoporous silica nanoparticles (MNPs) have been used to deliver aspirin to the tumor location. MNP-based aspirin in folic acid (F)-conjugated polydopamine (MNP-Asp-PD-PG-F) vehicles are prepared for targeted breast cancer therapy. The vehicle hinges on MNP altered with polymer polyethylene glycol (PG), polydopamine (PD), and F. The delivery vehicle was studied for in vitro drug release, cytotoxicity, and breast cancer cell proliferation. F-conjugated drug delivery vehicles let MNPs achieve an elevated targeting efficacy, ideal for cancer therapy. It was also observed that compared to free aspirin, our drug delivery system (MNP-Asp-PD-PG-F) has a higher cytotoxic and antiproliferative effect on breast cancer cells. The drug delivery system can be proposed as a targeted breast cancer therapy that could be further focused on other targeted cancer therapies. Delivering aspirin by the PD-PG-F system on the tumor sites promises a therapeutic potential for breast cancer treatment.
Apolipoprotein E Genotype Moderation of the Association Between Physical Activity and Brain Health. A Systematic Review and Meta-Analysis
Authors: Pearce Andrew M. , Marr Calum , Dewar Michaela , Gow Alan J.
Published: January 2022

Introduction

Possession of one or two e4 alleles of the apolipoprotein E (APOE) gene is associated with cognitive decline and dementia risk. Some evidence suggests that physical activity may benefit carriers of the e4 allele differently.

Method

We conducted a systematic review and meta-analysis of studies which assessed APOE differences in the association between physical activity and: lipid profile, Alzheimer's disease pathology, brain structure and brain function in healthy adults. Searches were carried out in PubMed, SCOPUS, Web of Science and PsycInfo.

Results

Thirty studies were included from 4,896 papers screened. Carriers of the e4 allele gained the same benefit from physical activity as non-carriers on most outcomes. For brain activation, e4 carriers appeared to gain a greater benefit from physical activity on task-related and resting-state activation and resting-state functional connectivity compared to non-carriers. Post-hoc analysis identified possible compensatory mechanisms allowing e4 carriers to maintain cognitive function.

Discussion

Though there is evidence suggesting physical activity may benefit e4 carriers differently compared to non-carriers, this may vary by the specific brain health outcome, perhaps limited to brain activation. Further research is required to confirm these findings and elucidate the mechanisms.
The Effect of Brief Stair-Climbing on Divergent and Convergent Thinking
Authors: Matsumoto Karin , Chen Chong , Hagiwara Kosuke , Shimizu Natsumi , Hirotsu Masako , Oda Yusuke , Lei Huijie , Takao Akiyo , Fujii Yuko , Higuchi Fumihiro , Nakagawa Shin
Published: January 2022

Recent studies show that even a brief bout of aerobic exercise may enhance creative thinking. However, few studies have investigated the effect of exercise conducted in natural settings. Here, in a crossover randomized controlled trial, we investigated the effect of a common daily activity, stair-climbing, on creative thinking. As experimental intervention, subjects were asked to walk downstairs from the fourth to the first floor and back at their usual pace. As control intervention, they walked the same path but using the elevator instead. Compared to using the elevator, stair-climbing enhanced subsequent divergent but not convergent thinking in that it increased originality on the Alternate Use Test (d = 0.486). Subjects on average generated 61% more original uses after stair-climbing. This is the first study to investigate the effect of stair-climbing on creative thinking. Our findings suggest that stair-climbing may be a useful strategy for enhancing divergent thinking in everyday life.
One PMP22/MPZ and Three MFN2/GDAP1 Concomitant Variants Occurred in a Cohort of 189 Chinese Charcot-Marie-Tooth Families
Authors: Xie Yongzhi , Lin Zhiqiang , Li Xiaobo , Liu Lei , Huang Shunxiang , Zhao Huadong , Wang Binghao , Cao Wanqian , Hu Zhengmao , Guo Jifeng , Shen Lu , Tang Beisha , Zhang Ruxu
Published: January 2022

Background and Aims

Charcot-Marie-Tooth (CMT) disease is a clinically and genetically heterogeneous group of inherited peripheral neuropathies. The wide phenotypic variability may not be completely explained by a single mutation.

Aims and Methods

To explore the existence of concomitant variants in CMT, we enrolled 189 patients and performed molecular diagnosis by application of next-generation sequencing combined with multiplex ligation-dependent probe amplification. We conducted a retrospective analysis of patients harboring coinherited variants in different genes.

Results

Four families were confirmed to possess variants in two genes, accounting for 2.1% (4/189) of the total in our cohort. One CMT1 patient with PMP22 duplication and MPZ variant (c.286A>C, p.K96Q) exhibited moderate neuropathy with infantile onset, while her father possessing MPZ variant was mildly affected with adolescence onset. A CMT2 patient with heterozygous variants in MFN2 (c.613_622delGTCACCACAG, p.V205Sfs*26) and GDAP1 (c.713G>T, p.W238L) exhibited childhood onset mild phenotype, while his mother with MFN2 variant developed bilateral pes cavus only. A CMT2 patient with heterozygous variants in MFN2 (c.839G>A, p.R280H) and GDAP1 (c.3G>T, p.M1?) presented infantile onset and rapid progression, while her father with MFN2 variant presented with absence of deep tendon reflexes. One sporadic CMT2 patient with early onset was confirmed harboring de novo MFN2 variant (c.1835C>T, p.S612F) and heterozygous GDAP1 variant (c.767A>G, p.H256R).

Conclusion

Our results suggest that the possibility of concomitant variants was not uncommon and should be considered when significant intrafamilial clinical heterogeneity is observed.
Neurologist-Led Management of Implantable Loop-Recorders After Embolic Stroke of Undetermined Source
Authors: Pikija Slaven , Rösler Cornelia , Leitner Ursula , Zellner Thomas , Bubel Nele , Ganser Bernhard , Hecker Constantin , Mutzenbach Johannes Sebastian
Published: January 2022

Introduction

Upon completion of the workup for stroke, etiology cannot be identified in approximately one-third of the patients, with an embolic stroke of undetermined source (ESUS) accounting for around 50% of these cryptogenic etiologies. Whether management of complex long-term monitoring in order to detect suspected atrial fibrillation (AFib) could be initiated and managed through a neurologist is not sufficiently investigated.

Patients and Methods

We recruited all consecutive patients with ESUS who received implantation after neurological adjudication of Reveal LINQ® loop recorder between January 2016 and July 2020. We collected demographic, clinical, heart- and neuroimaging, laboratory, and electrocardiographic data assessed on prolonged baseline ECG monitoring, number of supraventricular (SVEs) and ventricular (VEs) extrasystolic complexes, and from preimplantation ECG–PQ interval. AFib detection was manually supervised and determined positive when the duration was over 120 s.

Results

We followed a total of 131 patients for a median of 504 days. There were 45 (34%) manually verified AFib diagnoses. In univariate analysis, earlier implantation after ESUS was associated with AFib detection (13 vs. 31 days, p = 0.011). In multivariate analysis, increased rate of AFib was associated with a more prolonged PQ interval (per 50-ms increase) (HR 1.99, 95% CI 1.39–2.85) and number of SVEs (HR 1.29, 95% CI 1.05–1.57) measured on pre-implantation ECG.

Conclusion

We observed similar predictors for Afib after ESUS, albeit with higher frequency than previously reported. This study suggests that the neurologist-led decision, management, and evaluation of ILR after ESUS is feasible.
Macaque Area V2/V3 Reorganization Following Homonymous Retinal Lesions
Authors: Keliris Georgios A. , Shao Yibin , Schmid Michael C. , Augath Mark , Logothetis Nikos K. , Smirnakis Stelios M.
Published: January 2022

In the adult visual system, topographic reorganization of the primary visual cortex (V1) after retinal lesions has been extensively investigated. In contrast, the plasticity of higher order extrastriate areas following retinal lesions is less well studied. Here, we used fMRI to study reorganization of visual areas V2/V3 following the induction of permanent, binocular, homonymous retinal lesions in 4 adult macaque monkeys. We found that the great majority of voxels that did not show visual modulation on the day of the lesion in the V2/V3 lesion projection zone (LPZ) demonstrated significant visual modulations 2 weeks later, and the mean modulation strength remained approximately stable thereafter for the duration of our observations (4–5 months). The distribution of eccentricities of visually modulated voxels inside the V2/V3 LPZ spanned a wider range post-lesion than pre-lesion, suggesting that neurons inside the LPZ reorganize by receiving input either from the foveal or the peripheral border of the LPZ, depending on proximity. Overall, we conclude that area V2/V3 of adult rhesus macaques displays a significant capacity for topographic reorganization following retinal lesions markedly exceeding the corresponding capacity of area V1.