bluuebunny/crossref_metadata_2025 huggingface dataset:
Developmental Toxicity Study of DL-4-Hydroxy-4-Phenylhexanamide (DL-HEPB) in Rats
Authors: Cristóbal-Luna José Melesio , Mojica-Villegas María Angélica , Meza-Toledo Sergio Enrique , García-Martínez Yuliana , Pérez-Juárez Angélica , Chamorro-Cevallos Germán
Published: August 2023

Antiepileptic drugs affect embryonic development when administered during pregnancy, generating severe alterations, such as as cleft lip, spina bifida, heart abnormalities, or neuronal alterations. The compound DL-4-hydroxy-4-phenylhexanamide (DL-HEPB), a phenyl alcohol amide structurally different from known anticonvulsants, has shown good anticonvulsant effects in previous studies. However, its effects on intrauterine development are unknown. So, the purpose of this study was to determine the potential of DL-HEPB to produce alterations in conceptus. Pregnant Wistar rats were orally exposed to 0, 50, 100, and 200 mg/kg of DL-HEPB during organogenesis, and their food consumption and weight gain were measured. On gestation day 21, pregnant females were euthanized to analyze the fetuses for external, visceral, and skeletal malformations. A significant decrease in food consumption and body weight was observed in mothers, without any other manifestation of toxicity. In fetuses, no external malformations, visceral, or skeletal abnormalities, were observed under the dose of 100 mg/kg, while the dose of 200 mg/kg caused malformations in low frequency in brain and kidneys. In view of the results obtained, DL-HEPB could be a good starting point for the design of new highly effective anticonvulsant agents, with much lower developmental toxicity than that shown by commercial anticonvulsants.
Anesthetic Approaches and Perioperative Complications of Total Hip Arthroplasty in Gaucher Disease: A Control-Matched Retrospective-Cohort Study
Authors: Grass Ariel , Riemer Eyal , Zimran Ari , Revel-Vilk Shoshana , Freundlich Andres , Lebel Ehud , Ioscovich Alexander
Published: August 2023

Objectives: Gaucher disease’s (GD) pathophysiology generates anesthetic concerns in total hip joint arthroplasty (THA), and due to its rareness, data on perioperative risks are scarce. This 22-year study at a large reference center addresses anesthetic management and perioperative outcomes in GD. Methods: This retrospective-cohort study assessed anesthetic success and safety in 30 THA patients, comparing them with a control-matched group. Data on clinical characteristics, perioperative events, and outcomes were collected. The primary outcome was the success rate of anesthesia induction performance at first attempt. Secondary outcomes were difficult intraoperative course and hemodynamic management, and the development of postoperative complications. The age, sex, weight, body mass index, and primary-to-revision hip arthroplasty ratio were similar in both groups. Results: There was no significant difference at all-type anesthesia first initiation attempt success. No particular preference by staff anesthetists for general anesthesia or neuraxial procedures was observed. The GD group showed a significantly higher mean of intraoperative packed Red Blood Cell units administered ((0.73 vs. 0.18); (p = 0.038)), higher intraoperative and postoperative platelet transfusion incidence ((5/30 [16.7%] vs. 0/56 [0.00%]; p = 0.004) and (3/30 [10%] vs. 0/56 [0%]; p = 0.040)), and longer mean recovery room length of stay (426 ± 412 vs. 175 ± 140; p = 0.004). Postoperative complications were not significantly different.
Altitude Modifies the Effect of Parity on Birth Weight/Length Ratio: A Study Comprising 2,057,702 Newborns between 1984 and 2020 in Austria
Authors: Karner Eva , Muin Dana A. , Klebermass-Schrehof Katrin , Waldhoer Thomas , Yang Lin
Published: August 2023

(1) Background: Lower birth weight among newborns in higher altitudes has been well documented in previous literature. Several possible causes for this phenomenon have been investigated, including biophysiological adaptation, epigenetic or genetic mechanisms or lifestyle changes. This is the first study to show the effect modification of altitude and parity on the birth weight length ratio (BWLR) in women resident in moderate altitudes compared to a low sea level.; (2) Methods: This population-based study obtained data on altitude (0–300, 300–500, 500–700,700–900, >900 m), parity (1, 2, …, 7, 8/9), birth weight and length on all births in Austria between 1984 and 2020 from birth certificates provided by Statistics Austria. The BWLR was calculated, and the effect of moderate altitude and parity was estimated using multivariable linear mixed models adjusting for predefined variables. Sub-group regression analyses were conducted by altitude group. (3) Results: Data on 2,057,702 newborns from 1,280,272 mothers were analyzed. The effect of parity on BWLR, as indicated by the difference of BWLR between the first- and second-born infants, ranged between 1.87 to 2.09 g per centimeter across all altitude groups. Our analyses found that the effect of parity on BWLR diminished from parity three onwards at altitude 0–300, whilst the effect of parity on BWLR continued to increase at higher than 300 m and was most notable in the highest altitude group >900 m. (4) Conclusions: Findings from our study indicated that the negative effect of increasing altitude on BWLR was deprived for newborns of higher parity. It shows that the residential altitude can modify the effect of parity on BWLR.
Role of Perilesional Sampling of Patients Undergoing Fusion Prostate Biopsies
Authors: Lombardo Riccardo , Tema Giorgia , Nacchia Antonio , Mancini Elisa , Franco Sara , Zammitti Filippo , Franco Antonio , Cash Hannes , Gravina Carmen , Guidotti Alessio , Gallo Giacomo , Ghezzo Nicola , Cicione Antonio , Tubaro Andrea , Autorino Riccardo , De Nunzio Cosimo
Published: August 2023

Recently, researchers have proposed perilesional sampling during prostate biopsies to avoid systematic biopsies of patients at risk of prostate cancer. The aim of our study is to evaluate the role of perilesional sampling to avoid systematic biopsies of patients undergoing fusion biopsies. A prospective cohort of patients undergoing transrectal MRI transrectal fusion biopsies were consecutively enrolled. All the patients underwent systematic biopsies (SB), targeted biopsies (TB) and perilesional biopsies within 10 mm from the lesion (PB). The detection rates of different strategies were determined. A total of 262 patients were enrolled. The median age of those enrolled was 70 years. The mean BMI was 27 kg/m2, and the mean and prostate volume was 52 mL. A PIRADS score ≥ 4 was recorded in 163/262 (40%) patients. Overall, the detection rates of cancer were 43.5% (114/262) and 35% (92/262) for csPCa. The use of the target + peri-target strategy resulted in a detection of 32.8% (86/262) of cancer cases and of 29% (76/262) of csPCa cases (Grade Group > 2). Using the target plus peri-target approach resulted in us missing 18/262 (7%) of the csPCa cases, avoiding the diagnosis of 8/262 (3%) of nsPCa cases. A biopsy strategy including lesional and perilesional sampling could avoid unnecessary prostate biopsies. However, the risk of missing significant cancers is present. Future studies should assess the cost–benefit relationship of different strategies.
Recent Advances of Solutions Algorithms for Logistics Routing Problems
Authors: Babaee Tirkolaee Erfan Babaee , Goli Alireza , Malmir Behnam
Published: August 2023

Nowadays, the business landscape is highly focused on establishing efficient logistics systems, with numerous companies striving to gain a strong foothold in the global market and achieve maximum productivity and cost effectiveness [...]
Inhibition of Caries around Restoration by Ion-Releasing Restorative Materials: An In Vitro Optical Coherence Tomography and Micro-Computed Tomography Evaluation
Authors: Albelasy Eman H. , Chen Ruoqiong , Fok Alex , Montasser Marmar , Hamama Hamdi H. , Mahmoud Salah H. , Abdelrehim Tamer , Chew Hooi Pin
Published: August 2023

The objective of this study was firstly to assess the demineralization inhibitory effect of ion-releasing restorations in enamel adjacent to restoration using a biofilm caries model and secondly to compare the effect to that in a chemical caries model. Fifty-six bovine incisors were filled with either Surefil one (SuO), Cention N (CN) (both ion-releasing materials), Ketac-Molar (GIC) or Powerfill resin composite (RC). The restored teeth were then randomly divided into 2 groups according to the used caries model (biofilm or chemical caries model). The micro-computed tomography (MicroCt) and optical coherence tomography (OCT) outcome measures used to evaluate demineralization inhibition effects were lesion depth, LD and increase in OCT integrated reflectivity, ΔIR, at five different depths. It was observed that all outcome measures of CN were statistically the same as those of GIC and conversely with those of RC. This was also the case for SuO except for LD, which was statistically the same as RC. When comparing the two caries models, LD of the biofilm model was statistically deeper (p < 0.05) than the chemical model for all four materials. In conclusion, CN and SuO have similar demineralization inhibitory effects as GIC, and the biofilm caries model is more discriminatory in differentiating demineralization inhibitory effects of ion-releasing restorative material.
Topology of WC/Co Interfaces in Cemented Carbides
Authors: Straumal Boris B. , Shchur Lev N. , Kagramanyan David G. , Konstantinova Elizaveta P. , Druzhinin Alexander V. , Nekrasov Alexei N.
Published: August 2023

WC–Co cemented carbides build one of the important classes of metal matrix composites. We show in this paper that the use of machine vision methods makes it possible to obtain sufficiently informative statistical data on the topology of the interfaces between tungsten carbide grains (WC) and a cobalt matrix (Co). For the first time, the outlines of the regions of the cobalt binder were chosen as a tool for describing the structure of cemented carbides. Numerical processing of micrographs of cross sections of three WC–Co alloys, which differ in the average grain size, was carried out. The distribution density of the angles in the contours of cobalt “lakes” is bimodal. The peaks close to 110° (so-called outcoming angles) correspond to the contacts between the cobalt binder and the WC/WC grain boundaries. The peaks close to 240° (or incoming angles) correspond to the WC “capes” contacting the cobalt “lakes” and are determined by the angles between facets of WC crystallites. The distribution density of the linear dimensions of the regions of the cobalt binder, approximated with ellipses, were also obtained. The distribution density exponentially decreases with the lengths of the semi-axes of the ellipsoid, approximating the area of the cobalt binder. The possible connection between the obtained data on the shape of cobalt areas and the crack trajectories in cemented carbides is discussed.
The Differential Entropy Generation Rate as a Unified Measure for Both the Stability and Efficiency of an Axial Compressor
Authors: Ma Jingyuan , Lin Feng
Published: August 2023

Stability and efficiency are the two most important performance indicators of highly loaded aviation axial compressors; however, they often cannot be achieved simultaneously. As an effective means of stability expansion, casing treatment usually damages the peak efficiency. In this study, the differential entropy generation rate was used as a unified measure of stall margin and efficiency, so that both stability enhancement and efficiency improvement could be considered at the initial casing treatment design stage. NASA Rotor 67 was selected, and two single circumferential grooves at two different axial positions were applied, which served as a test case to check how entropy generation rates in the flow field vary with changes in peak efficiency and stall margin. The distribution of entropy generation and differential entropy generation rate were compared with that of the solid casing. The correlation between differential entropy generation rate and the peak efficiency was analyzed, and how the flow mechanism of casing treatments affects entropy generation was determined. Methods for measuring and comparing the impact of different casing treatments on peak efficiency are proposed. At the same time, the distributions of differential entropy generation rate in the near stall were explored, and the relationships between the differential entropy generation rate and the flow structures are detailed. A comparison of the influence of different casing treatment on stability is given with respect to the contours of the differential entropy generation rates. It is demonstrated that the differential entropy generation rate is a unified measure to balance the tradeoffs between the stability and the peak efficiency for different casing treatments for the same compressor.
High-Performance Lightweight Fall Detection with an Improved YOLOv5s Algorithm
Authors: Wang Yuanpeng , Chi Zhaozhan , Liu Meng , Li Guangxian , Ding Songlin
Published: August 2023

The aging population has drastically increased in the past two decades, stimulating the development of devices for healthcare and medical purposes. As one of the leading potential risks, the injuries caused by accidental falls at home are hazardous to the health (and even lifespan) of elderly people. In this paper, an improved YOLOv5s algorithm is proposed, aiming to improve the efficiency and accuracy of lightweight fall detection via the following modifications that elevate its accuracy and speed: first, a k-means++ clustering algorithm was applied to increase the accuracy of the anchor boxes; the backbone network was replaced with a lightweight ShuffleNetV2 network to embed simplified devices with limited computing ability; an SE attention mechanism module was added to the last layer of the backbone to improve the feature extraction capability; the GIOU loss function was replaced by a SIOU loss function to increase the accuracy of detection and the training speed. The results of testing show that the mAP of the improved algorithm was improved by 3.5%, the model size was reduced by 75%, and the time consumed for computation was reduced by 79.4% compared with the conventional YOLOv5s. The algorithm proposed in this paper has higher detection accuracy and detection speed. It is suitable for deployment in embedded devices with limited performance and with lower cost.
Dynamic Characteristics of the Chain Drive System under Multiple Working Conditions
Authors: Jiang Shoubo , Huang Shuan , Zeng Qingliang , Chen Shaojie , Lv Jinwang , Zhang Yuqi , Qu Wei
Published: August 2023

The chain drive system, being one of the core subsystems of the scraper conveyor, greatly influences the coal-conveying efficiency due to its dynamic characteristics. Therefore, this paper employed an experimental method to compare the dynamic characteristics of the chain rings at various positions within the chain drive system. This article simulated the three most common working conditions. The results indicated that the chain speed, load, and surface undulation had a stimulating effect on chain vibration, with the most significant impact observed on the chain ring located in the middle of the adjacent scraper. The scraper exerted an inhibitory effect on the vibration of the chain ring. Among the factors studied, the load had the most significant influence on the transverse direction. When there was surface undulation, the vibration of the chain ring in the running direction formed an ‘N’ shape. This condition intensified the vibration during uphill and downhill stages while suppressing vibration at the slope peak. This research holds great significance in enhancing operation stability.
Investigation of the Layer Effects Formed by W-EDM on Electrochemical Grooving of Stellite 21
Authors: Anil Semih Ekrem , Demirtas Hasan , Kalayci Adnan , Cebi Abdulkadir
Published: August 2023

Machining hard-to-cut materials, such as cobalt (Co)-based superalloys, is a common problem in manufacturing industries. Background: wire electrical discharge machining (W-EDM) is one of the widely used cutting processes that causes layer (white layer—WL and heat-affected zone—HAZ) formation, and microcracks on the material’s surface. Purpose: this study investigates the effects of WL and HAZ on the electrochemical grooving (EC grooving) performance of Co-based superalloys. Two different surface types (W-EDMed and VFed) were used in the experiments. Result: the experiments showed that material removal rate (MRR) values increased up to 212.49% and 122.23% for vibratory finished (VFed) and wire-electrical-discharge-machined (W-EDMed) surfaces, respectively. Conclusion: This result indicates the presence of HAZ and WL that prevent current transition between two electrodes. However, increased voltage causes an increase in surface roughness, with increment rates at 71.13% and 36.08% for VFed and W-EDMed surfaces, respectively. Moreover, for the VFed surfaces, the groove lost its flatness at the bottom after an approximately 100 µm depth due to the different electrochemical machineabilities of HAZ and real surface texture. This result can be attributed to the different microstructures (HAZ and surface texture) showing different electrochemical dissolution rates. Therefore, high-depth distance HAZ and WL must be removed from the workpiece.
Improvement of Constructal Optimization for “Volume-Point” Heat Conduction Based on Uniformity Principle of Temperature Difference Fields
Authors: Wei Shuhuan , Wang Dini
Published: August 2023

The uniformity principle of temperature difference fields (TDFs) is applied in this study to improve the constructal optimization for “volume-point” heat conduction based on entransy dissipation rate (EDR) minimization without the premise of an optimal last-order construct, and the constructal optimization algorithm based on EDR minimization is simplified in this paper. The results further prove that the uniformity principle of TDF is consistent with entransy theory. The constructal optimization of “volume-point” heat conduction based on EDR minimization is conducted not only to lower the average temperature but also to obtain a more uniform TDF distribution. Through comparing the optimal results based on EDR minimization without the premise of an optimal last-order construct with those based on maximum temperature difference (MTD) minimization, some criteria and formulas for designing conductivity paths based on EDR minimization and MTD minimization are proposed, and the idea and method of improving constructal optimization via the variational principle are proposed.
Two-Step Adaptive Control for Planar Type Docking of Autonomous Underwater Vehicle
Authors: Wang Tianlei , Sun Zhenxing , Ke Yun , Li Chaochao , Hu Jiwei
Published: August 2023

Planar type docking enables a convenient underwater energy supply for irregularly shaped autonomous underwater vehicles (AUVs), but the corresponding control method is still challenging. Conventional control methods for torpedo-shaped AUVs are not suitable for planar type docking due to the significant differences in system structures and motion characteristics. This paper proposes a two-step adaptive control method to solve the planar type docking problem. The method makes a seamless combination of horizontal dynamic positioning and visual servo docking considering ocean current disturbance. The current disturbance is estimated and canceled in the pre-docking step using a current observer, and the positioning error is further compensated for by the vertical visual servo technique in the docking step. Reduced order dynamic models are distinctively established for different docking steps according to the motion characteristics, based on which the dynamic controllers are designed considering the model parameter uncertainties. Simulation is conducted with an initial distance of 10 m in the horizontal direction and 3 m in depth. Stable and accurate dynamic positioning under up to 0.4 m/s of current disturbances with different directions is validated. A 0.5 m lateral positioning error is successfully compensated for by the visual servo docking step. The proposed control method provides a valuable reference for similar types of docking application.
Overview and Challenges of Large-Scale Cultivation of Photosynthetic Microalgae and Cyanobacteria
Authors: Novoveská Lucie , Nielsen Søren Laurentius , Eroldoğan Orhan Tufan , Haznedaroglu Berat Zeki , Rinkevich Baruch , Fazi Stefano , Robbens Johan , Vasquez Marlen , Einarsson Hjörleifur
Published: August 2023

Microalgae and cyanobacteria are diverse groups of organisms with great potential to benefit societies across the world. These organisms are currently used in food, feed, pharmaceutical and cosmetic industries. In addition, a variety of novel compounds are being isolated. Commercial production of photosynthetic microalgae and cyanobacteria requires cultivation on a large scale with high throughput. However, scaling up production from lab-based systems to large-scale systems is a complex and potentially costly endeavor. In this review, we summarise all aspects of large-scale cultivation, including aims of cultivation, species selection, types of cultivation (ponds, photobioreactors, and biofilms), water and nutrient sources, temperature, light and mixing, monitoring, contamination, harvesting strategies, and potential environmental risks. Importantly, we also present practical recommendations and discuss challenges of profitable large-scale systems associated with economical design, effective operation and maintenance, automation, and shortage of experienced phycologists.
Water Molecules’ and Lithium Cations’ Mobility in Sulfonated Polystyrene Studied by Nuclear Magnetic Resonance
Authors: Bilyk Stepan , Tverskoy Vladimir , Chernyak Alexander , Avilova Irina , Slesarenko Nikita , Volkov Vitaly
Published: August 2023

The hydration of ions and charge groups controls electro mass transfer through ion exchange systems. The self-diffusion and local mobility of water molecules as well as lithium cations in poly (4-styrenesulfonic acid) and its lithium, sodium and cesium salts were investigated for the first time using pulsed-field gradient NMR (PFG NMR) and NMR relaxation techniques. The temperature dependences of the water molecule and Li+ cation self-diffusion coefficients exhibited increasing self-diffusion activation energy in temperature regions below 0 °C, which is not due to the freezing of parts of the water. The self-diffusion coefficients of water molecules and lithium cations, as measured using PFG NMR, are in good agreement with the self-diffusion coefficients calculated based on Einstein’s equation using correlation times obtained from spin-lattice relaxation data. It was shown that macroscopic water molecules’ and lithium cations’ transfer is controlled by local particles jumping between neighboring sulfonated groups. These results are similar to the behavior of water and cations in sulfonic cation exchanger membranes and resins. It was concluded that polystyrenesulfonic acid is appropriate model of the ionogenic part of membranes based on this polymer.
Structure–Phase Transitions in the Friction Contact Zone of High-Nitrogen Chromium–Manganese Austenitic Steel
Authors: Shabashov Valery , Korshunov Lev , Kozlov Kirill , Lyashkov Kirill , Zamatovskii Andrey , Dorofeev Gennady , Kataeva Natalya
Published: August 2023

The influence of contact stresses on the phase and concentration composition of thin surface layers and wear products in the tribological contact zone of high-nitrogen FeMn22Cr18N0.83 steel was studied using Mössbauer spectroscopy, X-ray structural analysis, and electron microscopy. It was shown that contact compressive stresses developing under the conditions of dry sliding friction in the surface layers (20–25 microns) resulted in the strain-induced dissolution of cellular precipitation products (nitrides Cr2N) and increased the average content of nitrogen in austenite. Antiferromagnetic ordering in austenite caused by the precipitation of secondary nitrides with low chromium and nitrogen content was observed in tiny external layers (~0.1 microns) of the friction surface and products of steel adhesive wear. The effect of tension stresses in the friction contact zone on the formation of strain-induced martensite and nitrides with α″-Fe16N2 structures was established in the wear products.
Structure Investigation of La, Y, and Nd Complexes in Solvent Extraction Process with Liquid Phosphine Oxide, Phosphinic Acid, and Amine Extractants
Authors: Botelho Junior Amilton Barbosa , da Silva Natália Olim Martins , Tenório Jorge Alberto Soares , Espinosa Denise Crocce Romano
Published: August 2023

The main challenge in separating REEs through hydrometallurgical processes is their chemical similarities. Despite the literature widely presenting the possibilities for organic extractants, there is a lack of evaluation of the structures formed between the REEs and the extractants. The present study aimed to evaluate different extractants (neutral, anionic, and acid extractants) for separating La, Y, and Nd. The extraction efficiencies were evaluated, and the structure investigation was carried out in FT-IR. From the results obtained, it is clear that the extraction order is Alamine 336 <<< Cyanex 272 < Cyanex 923, where both acid extractants were more selective for Y than for La and Nd. The extraction achieved 99% at pH 5.0 in nitric acid media, and a Y/La ratio of 2 and a Y/Nd ratio of 4 using Cyanex 923. The present study also elucidated the organometallic complexation between Cyanex 923 and Cyanex 272 with Y and La, which may improve separation processes to obtain critical metals from primary and secondary sources.
Optimization of Macro Segregation and Equiaxed Zone in High-Carbon Steel Use in Prestressed Concrete Wire and Cord Wire Application
Authors: Ayçiçek İlker , Solak Nuri
Published: August 2023

In this study, the relationship between macro segregation and the equiaxed zone in high-carbon grades with continuous casting parameters was investigated and optimized at the İsdemir iron and steel plant. The work was conducted for the 1080 quality of the SAE J403 standard. In this study, some parameters, such as casting speed, secondary cooling, EMS current value and EMS frequency value, were examined. When the results of the experiments are examined, it can be observed that the equiaxed zone in the macrostructure decreases significantly with the reduction of the EMS frequency value. The decrease in casting speed and increase in EMS current value caused an increase in the equiaxed zone. The increment in secondary cooling led to a decline in the equiaxed zone. Once the macro segregation results are examined, it can be seen that it is very important to optimize the continuous casting parameters in order to reduce the macro segregation results of—especially—carbon, sulfur and phosphorus elements. It has also been determined that the macro segregation values of carbon, sulfur and phosphorus elements are low in casting conditions where casting speed is low, and the EMS current value and EMS frequency value are high. In addition, macro segregation measurements of manganese, silicon, chromium and vanadium elements are found to be low under similar casting conditions. It is critical to optimize the continuous casting parameters before production, especially in high-carbon grades to be used for prestressed concrete wire and cord wire applications. As a result of the work conducted using the İsdemir billet continuous casting machine for the 1080-grade SAE J403 standard, aiming to optimize macro segregation and the equiaxed zone, the effective results have been achieved by using process parameters of 2.8 m/min casting speed, 360 A EMS current, 5 Hz EMS frequency and low secondary cooling intensity.
Biostimulant and Bioinsecticidal Effect of Coating Cotton Seeds with Endophytic Beauveria bassiana in Semi-Field Conditions
Authors: Mantzoukas Spiridon , Papantzikos Vasileios , Katsogiannou Spiridoula , Papanikou Areti , Koukidis Charalampos , Servis Dimitrios , Eliopoulos Panagiotis , Patakioutas George
Published: August 2023

Increasing commercial demands from the textile and food industries are putting strong pressure on the cultivation of cotton and its derivatives to produce high yields. At the same time, cotton has high nutrient and irrigation requirements and is highly susceptible to insect pests. Coating cotton seeds with beneficial fungi could address these problems. The aim of this study was to investigate the growth of cotton using (A) conventional seeds and (B) seeds coated with the entomopathogenic fungus Beauveria bassiana (Hypocreales: Cordycipitaceae). The experiment was conducted in a greenhouse of the Department of Agriculture of the University of Ioannina, in a completely randomized design. The growth characteristics of cotton plants were recorded weekly while the fresh weight and dry matter of the leaves, shoots and roots of the developed cotton plants were calculated at the end of the experiment. Weekly determination of total chlorophyll content (TCHL) was used as an indicator of plant robustness during the 80-day experiment. Many cotton growth parameters of treated plants, like number of leaves, shoots and apical buds, plant height, stem diameter, fresh and dried biomass and TCHL, were significantly higher than those of the untreated ones. Apart from plant growth, naturally occurring by Aphis gossypii (Hemiptera: Aphididae) infestation which also monitored for six weeks. A significantly lower aphid population was recorded for inoculated plants after the fifth week compared to the control. The overall evaluation revealed that B. bassiana coating treatments appear to have a significant biostimulatory and bioinsecticidal effect. Our results could represent responsive applications to the demands of intensive cotton growing conditions.
Taxonomy, Sequence Variance and Functional Profiling of the Microbial Community of Long-Ripened Cheddar Cheese Using Shotgun Metagenomics
Authors: Mohamed Hassan Mahmoud , Barzideh Zoha , Siddiqi Myra , LaPointe Gisèle
Published: August 2023

Shotgun metagenomic sequencing was used to investigate the diversity of the microbial community of Cheddar cheese ripened over 32 months. The changes in taxa abundance were compared from assembly-based, non-assembly-based, and mOTUs2 sequencing pipelines to delineate the community profile for each age group. Metagenomic assembled genomes (MAGs) passing the quality threshold were obtained for 11 species from 58 samples. Although Lactococcus cremoris and Lacticaseibacillus paracasei were dominant across the shotgun samples, other species were identified using MG-RAST. NMDS analysis of the beta diversity of the microbial community revealed the similarity of the cheeses in older age groups (7 months to 32 months). As expected, the abundance of Lactococcus cremoris consistently decreased over ripening, while the proportion of permeable cells increased. Over the ripening period, the relative abundance of viable Lacticaseibacillus paracasei progressively increased, but at a variable rate among trials. Reads attributed to Siphoviridae and Ascomycota remained below 1% relative abundance. The functional profiles of PMA-treated cheeses differed from those of non-PMA-treated cheeses. Starter rotation was reflected in the single nucleotide variant profiles of Lactococcus cremoris (SNVs of this species using mOTUs2), while the incoming milk was the leading factor in discriminating Lacticaseibacillus paracasei/casei SNV profiles. The relative abundance estimates from Kraken2, non-assembly-based (MG-RAST) and marker gene clusters (mOTUs2) were consistent across age groups for the two dominant taxa. Metagenomics enabled sequence variant analysis below the bacterial species level and functional profiling that may affect the metabolic interactions between subpopulations in cheese during ripening, which could help explain the overall flavour development of cheese. Future work will integrate microbial variants with volatile profiles to associate the development of compounds related to cheese flavour at each ripening stage.
Antifungal Activity of Industrial Bacillus Strains against Mycogone perniciosa, the Causative Agent of Wet Bubble Disease in White Button Mushrooms
Authors: Novikova Irina , Titova Julia
Published: August 2023

White button mushrooms yield instability in artificial cultivation, often caused by crop diseases. The main disease is wet bubble disease. The appearance of its causative agent, M. perniciosa, may lead to total yield loss. The microbiocontrol of M. perniciosa is focused on casing soil antagonist use. Since no industrial producer strains of polyfunctional biologics have been used in previous studies, our research goal was to characterize the effect of B. subtilis B-10 and M-22 on a mycopathogen and reveal its control possibilities. The reason for B. subtilis B-10 and M-22 use in mycopathogen control has been revealed by interactions between producer strains and the studying of M. perniciosa. The suppression of M. perniciosa development by producer strains was established, indicating a prolonged B-10 and M-22 effect on the mycopathogen. High biological efficacy in both strains at the early stages of mycopathogen development upon introducing them into the wells and spraying was shown: B-10—50.9–99.6% and M-22—57.5–99.2%, respectively (p ≤ 0.05). Significant differences between producer strains were not revealed, although during the first day of exposure to developed M. perniciosa colonies, M-22 showed greater activity. The high efficiency of preventive treatment when producer strains completely suppressed mycoparasite development permits us to recommend them both for introducing when preparing casing for M. perniciosa control.
Toxoplasma gondii Seroprevalence and Trends in Women Presenting for Toxoplasma Screening in South-West Romania
Authors: Radoi Cristiana Luiza , Zlatian Ovidiu Mircea , Balasoiu Maria , Giubelan Lucian , Stoian Andreea Cristina , Dragonu Livia , Neacsu Alexandru , Iliescu Dominic Gabriel
Published: August 2023

Toxoplasmosis, caused by the obligate intracellular protozoan parasite Toxoplasma gondii (T. gondii), is a globally prevalent zoonotic disease with potentially severe implications for immunocompromised individuals, pregnant women, and their fetuses/children. This study examined the prevalence of anti-T. gondii IgM and IgG antibodies in two groups of childbearing age women, including 653 participants in Group 1 (2013–2016) and 3221 participants in Group 2 (2019–2022). Our results revealed a decrease in the overall positivity rate of anti-T. gondii IgM antibodies from 2.32% to 1.06%, suggesting improved public health interventions over time. However, there were variations among different age groups and between rural and urban environments, with a significant decrease in urban areas across all age groups from Group 1 to Group 2. Regarding anti-T. gondii IgG antibodies, we did not observe a significant change in the seropositivity rate between the two groups. In the rural population with an age group over 35 years, we observed the highest positivity rate in Group 2. This study provided information on the risk factors and burden of toxoplasmosis in women of childbearing age with data that can be valuable to public health policies and the planning of healthcare measures for effective toxoplasmosis management.
Structural Modifications and Novel Protein-Binding Sites in Pre-miR-675—Explaining Its Regulatory Mechanism in Carcinogenesis
Authors: Dey Abhishek
Published: August 2023

Pre-miR-675 is a microRNA expressed from the exon 1 of H19 long noncoding RNA, and the atypical expression of pre-miR-675 has been linked with several diseases and disorders including cancer. To execute its function inside the cell, pre-miR-675 is folded into a particular conformation, which aids in its interaction with several other biological molecules. However, the exact folding dynamics of pre-miR-675 and its protein-binding motifs are currently unknown. Moreover, how H19 lncRNA and pre-miR-675 crosstalk and modulate each other’s activities is also unclear. The detailed structural analysis of pre-miR-675 in this study determines its earlier unknown conformation and identifies novel protein-binding sites on pre-miR-675, thus making it an excellent therapeutic target against cancer. Co-folding analysis between H19 lncRNA and pre-miR-675 determine structural transformations in pre-miR-675, thus describing the earlier unknown mechanism of interaction between these two molecules. Comprehensively, this study details the conformation of pre-miR-675 and its protein-binding sites and explains its relationship with H19 lncRNA, which can be interpreted to understand the role of pre-miR-675 in the development and progression of tumorigenesis and designing new therapeutics against cancers.
Methods and Tools in RNA Biology
Authors: Ilieva Mirolyuba , Uchida Shizuka
Published: August 2023

Breakthroughs in innovative techniques and instruments have driven the exploration of non-coding RNAs (ncRNAs), including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) [...]
A Randomized Double-Blind Placebo-Controlled Clinical Study to Evaluate the Effect on the Weight of a Medical Device with Polyglucosamine L112 in a Group of Overweight and Obese Subjects
Authors: Rondanelli Mariangela , Perna Simone , Della Porta Matteo , Lombardoni Federico , Patelli Zaira , Nichetti Mara , Gasparri Clara , Pistolesi Elvira , Cestaro Benvenuto , Cazzola Roberta
Published: August 2023

Background. Overweight and obesity have reached epidemic proportions and safe treatments are needed to heal these diseases. Objective. The objective of this study is to examine the activity of a medical device based on polyglucosamine polymers (PG) on body weight (BW) reduction, insulin resistance, and the serum levels of fat-soluble vitamins and glucosamine. Methods. A double-blind placebo-controlled interventional study comparing PG and a placebo (PL) was conducted. One hundred and fifty overweight or obese cases were treated, divided into two groups for a period of 90 days at the dosage of 3 g/day. Results. One hundred and nineteen cases (58 with PG and 61 with PL, respectively) concluded the treatment. PG was more effective than the PL on the reduction of BW and insulin resistance. No modification of fat-soluble vitamins (Vit A, E, D3, K1) and glucosamine levels was shown. Total cholesterol levels were significantly more reduced in the PG group compared to the PL group as it was for subjects with a BW decrease of >5%. Conclusions. PG acts as a safe medical device, is not absorbed, and binds lipids in the upper gastrointestinal tract, reducing their availability, with a significant activity on the reduction of BW, insulin resistance, and cholesterol levels without the modification of fat-soluble vitamins.
Protective Effects of Bacteriocin-Producing Lactiplantibacillus plantarum on Intestinal Barrier of Mice
Authors: Bu Yushan , Liu Yisuo , Liu Yinxue , Cao Jiayuan , Zhang Zhe , Yi Huaxi
Published: August 2023

Bacteriocins are crucial metabolites of probiotics that display beneficial functions. The intestinal barrier is an important target on which probiotics exert their intestinal health activity. However, the impacts of bacteriocin-producing probiotics on the intestinal barrier are unclear. In this study, the effects of bacteriocin-producing Lactiplantibacillus plantarum Q7 and L. plantarum F3-2 on the intestinal barrier of mice were explored. It was shown that L. plantarum Q7 promoted the expression of mucin MUC2 to enhance the protection provided by the intestinal mucus layer. L. plantarum Q7 up-regulated the gene expression of intestinal tight junction proteins ZO-1 and JAM-1 significantly, and L. plantarum F3-2 up-regulated ZO-1 and Claudin-1 markedly, which exhibited tight junction intestinal barrier function. The two strains promoted the release of IgA and IgG at varying degrees. The antimicrobial peptide gene RegIIIγ was up-regulated markedly, and the gene expression of inflammatory cytokines appeared to exhibit an upward trend with L. plantarum Q7 treatment, so as to enhance intestinal immune regulation function. Furthermore, L. plantarum Q7 and L. plantarum F3-2 increased the abundance of the beneficial bacteria Muribaculaceae, inhibited the growth of the harmful bacteria Parabacteroides, and facilitated the synthesis of total short-chain fatty acids (SCFAs), which seemed to favor the prevention of metabolic diseases. Our results suggested that L. plantarum Q7 and L. plantarum F3-2 showed strain specificity in their protective effects on the intestinal chemical, physical, immunological and biological barriers of mice, which provided theoretical support for the selective utilization of bacteriocin-producing strains to regulate host health.
Brain Tuberculosis: An Odyssey through Time to Understand This Pathology
Authors: Patrascu Raluca Elena , Cucu Andrei Ionut , Costea Claudia Florida , Cosman Mihaela , Blaj Laurentiu Andrei , Hristea Adriana
Published: August 2023

Tuberculosis is a contagious disease that has been a concern for humanity throughout history, being recognized and referred to as the white plague. Since ancient times, starting with Hippocrates and Galen of Pergamon, doctors and scientists have attempted to understand the pathogenesis of tuberculosis and its manifestations in the brain. If, in the medieval period, it was believed that only the touch of a king could cure the disease, it was only in the early 17th and 18th centuries that the first descriptions of tuberculous meningitis and the first clinico-pathological correlations began to emerge. While the understanding of neurotuberculosis progressed slowly, it was only after the discovery of the pathogenic agent in the late 19th century that there was an upward curve in the occurrence of treatment methods. This review aims to embark on an odyssey through the centuries, from ancient Egypt to the modern era, and explore the key moments that have contributed to the emergence of a new era of hope in the history of neurotuberculosis. Understanding the history of treatment methods against this disease, from empirical and primitive ones to the emergence of new drugs used in multi-drug-resistant tuberculosis, leads us, once again, to realize the significant contribution of science and medicine in treating a disease that was considered incurable not long ago.
Anaplasma phagocytophilum Ecotype Analysis in Cattle from Great Britain
Authors: Apaa Ternenge Thaddaeus , McFadzean Harriet , Gandy Sara , Hansford Kayleigh , Medlock Jolyon , Johnson Nicholas
Published: August 2023

Anaplasma phagocytophilum (A. phagocytophilum) is the aetiological agent of tick-borne fever in cattle and sheep, and granulocytic anaplasmosis in human and dogs. Livestock, companion animal and human infections with A. phagocytophilum have been reported globally. Across England and Wales, two isolates (called ecotypes) have been reported in ticks. This study examined A. phagocytophilum isolates present in livestock and wildlife in Great Britain (GB), with a particular focus on cattle. Clinical submissions (EDTA blood) from cattle (n = 21) and sheep (n = 3) were received by APHA for tick-borne disease testing and the animals were confirmed to be infected with A. phagocytophilum using a PCR targeting the Msp2 gene. Further submissions from roe deer (n = 2), red deer (n = 2) and Ixodes ricinus ticks (n = 22) were also shown to be infected with A. phagocytophilum. Subsequent analysis using a nested PCR targeting the groEL gene and sequencing confirmed the presence of ecotype I in cattle, sheep, red deer and Ixodes ricinus, and ecotype II in roe deer and I. ricinus removed from deer carcasses. Despite the presence of two ecotypes, widely distributed in ticks from England and Wales, only ecotype I was detected in cattle in this study.
Purification Effect of PES-C Ultrafiltration Membrane Incorporated with Emodin on Acanthopanax Senticosus Injection
Authors: Zhang Jie , Zhang Chunyan , Xue Hongdan , Lu Chengbo , Rong Rong , Li Jinjing , Zhou Shujing
Published: August 2023

A new PES-C/emodin ultrafiltration membrane was prepared by blending natural emodin with phenolphthalein polyethersulfone (PES-C) and was used to purify an acanthopanax senticosus injection in this study. Regarding the purified acanthopanax senticosus injection, its color became lighter, and its clarity increased. On the contrary, for an acanthopanax senticosus injection containing macromolecules, its color deepened, and its turbidity increased. Thermal stability of the purified acanthopanax senticosus injection was the best, followed by the original solution of the acanthopanax senticosus injection, and the acanthopanax senticosus injection containing macromolecules was the worst. The fingerprint spectrum of the purified acanthopanax senticosus injection was similar to the original solution of the acanthopanax senticosus injection, the relative peak area of each single peak was greater than 0.95, and the relative peak area of the total peak was greater than 0.96. Compared with the original solution of the acanthopanax senticosus injection, the histamine release amount and cell degranulation rate of the acanthopanax senticosus injection containing macromolecules increased, while those of the purified acanthopanax senticosus injection decreased, which reduced the risk of allergic reaction to a certain extent. “Inverse proof” confirmed that the acanthopanax senticosus injection containing macromolecules had certain liver and kidney toxicity, which indirectly proved that the liver and kidney toxicity of the purified acanthopanax senticosus injection was effectively reduced.
A Review of the Role of Natural Products as Treatment Approaches for Xerostomia
Authors: Kontogiannopoulos Konstantinos N. , Kapourani Afroditi , Gkougkourelas Ioannis , Anagnostaki Maria-Emmanouela , Tsalikis Lazaros , Assimopoulou Andreana N. , Barmpalexis Panagiotis
Published: August 2023

Xerostomia, commonly known as dry mouth, is a widespread oral health malfunction characterized by decreased salivary flow. This condition results in discomfort, impaired speech and mastication, dysphagia, heightened susceptibility to oral infections, and ultimately, a diminished oral health-related quality of life. The etiology of xerostomia is multifaceted, with primary causes encompassing the use of xerostomic medications, radiation therapy to the head and neck, and systemic diseases such as Sjögren’s syndrome. Consequently, there is a growing interest in devising management strategies to address this oral health issue, which presents significant challenges due to the intricate nature of saliva. Historically, natural products have served medicinal purposes, and in contemporary pharmaceutical research and development, they continue to play a crucial role, including the treatment of xerostomia. In this context, the present review aims to provide an overview of the current state of knowledge regarding natural compounds and extracts for xerostomia treatment, paving the way for developing novel therapeutic strategies for this common oral health issue.