bluuebunny/crossref_metadata_2025 huggingface dataset:
No effect of the endurance training status on senescence despite reduced inflammation in skeletal muscle of older individuals
Authors: Balan Estelle , De Groote Estelle , Bouillon Margot , Viceconte Nikenza , Mahieu Manon , Naslain Damien , Nielens Henri , Decottignies Anabelle , Deldicque Louise
Published: July 2020

The aim of the present study was to determine if the training status decreases inflammation, slows down senescence, and preserves telomere health in skeletal muscle in older compared with younger subjects, with a specific focus on satellite cells. Analyses were conducted on skeletal muscle and cultured satellite cells from vastus lateralis biopsies ( n = 34) of male volunteers divided into four groups: young sedentary (YS), young trained cyclists (YT), old sedentary (OS), and old trained cyclists (OT). The senescence state and inflammatory profile were evaluated by telomere dysfunction-induced foci (TIF) quantification, senescence-associated β-galactosidase (SA-β-Gal) staining, and quantitative (q)RT-PCR. Independently of the endurance training status, TIF levels (+35%, P < 0.001) and the percentage of SA-β-Gal-positive cells (+30%, P < 0.05) were higher in cultured satellite cells of older compared with younger subjects. p16 (4- to 5-fold) and p21 (2-fold) mRNA levels in skeletal muscle were higher with age but unchanged by the training status. Aging induced higher CD68 mRNA levels in human skeletal muscle (+102%, P = 0.009). Independently of age, both trained groups had lower IL-8 mRNA levels (−70%, P = 0.011) and tended to have lower TNF-α mRNA levels (−40%, P = 0.10) compared with the sedentary subjects. All together, we found that the endurance training status did not slow down senescence in skeletal muscle and satellite cells in older compared with younger subjects despite reduced inflammation in skeletal muscle. These findings highlight that the link between senescence and inflammation can be disrupted in skeletal muscle.
A Structural Review of Thermoelectricity for Fuel Cell CCHP Applications
Authors: Bayendang Nganyang Paul , Kahn Mohamed Tariq , Balyan Vipin
Published: July 2020

This article starts by introducing the ongoing South Africa electricity crisis followed by thermoelectricity, in which eighteen miscellaneous applicable case studies are structurally analysed in detail. The aim is to establish best practices for the R&D of an efficient thermoelectric (TE) and fuel cell (FC) CCHP system. The examined literature reviews covered studies that focused on the thermoelectricity principle, highlighting TE devices’ basic constructions, TEGs and TECs as well as investigations on the applications of thermoelectricity with FCs, whereby thermoelectricity was applied to recover waste heat from FCs to boost the power generation capability by ~7–10%. Furthermore, nonstationary TEGs whose generated power can be increased by pulsing the DC-DC power converter showed that an output power efficiency of 8.4% is achievable and that thicker TEGs with good area coverage can efficiently harvest waste heat energy in dynamic applications. TEG and TEC exhibit duality and the higher the TEG temperature difference, the more the generated power—which can be stabilised using the MPPT technique with a 1.1% tracking error. A comparison study of TEG and solar energy demonstrated that TEG generates more power compared to solar cells of the same size, though more expensively. TEG output power and efficiency in a thermal environment can be maximised simultaneously if its heat flux is stable but not the case if its temperature difference is stable. The review concluded with a TEC LT-PEM-FC hybrid CCHP system capable of generating 2.79 kW of electricity, 3.04 kW of heat, and 26.8 W of cooling with a total efficiency of ~77% and fuel saving of 43.25%. The presented research is the contribution brought forward, as it heuristically highlights miscellaneous thermoelectricity studies/parameters of interests in a single manuscript, which further established that practical applications of thermoelectricity are possible and can be innovatively applied together with FC for efficient CCHP applications.
Reliability Assessment Based on GO Method of Metro Traction System
Authors: Gong Qi , Chen Bingzhi , Li Yonghua , Zhi Pengpeng
Published: July 2020

In order to improve the reliability of the metro traction system (MTS), the whole life cycle of metro vehicles can be operated safely and reliably. A reliability assessment method based on the GO method of the MTS is proposed. In this paper, the reliability assessment is completed without operation service failure of the metro vehicle. The maintainability and shutdown correlation of many electrical components in the MTS are considered. An accurate algorithm with shared signals is used. Then the quantitative and qualitative reliability assessment of the system is achieved with the goal of traction system providing traction for the vehicle. The evaluation identifies the weak links of the system. Comparing the completely independent quantitative calculation result of each component with the accurate quantitative calculation results, it is found that the complex correlation in the repairable system has an important effect on the reliability of the system. A comparative analysis is performed on the calculating results of the GO method and fault tree analysis (FTA) method. The results demonstrate that the reliability analysis of the MTS by the GO method is feasible and reasonable. The principle of the method is simple and the logic is clear. It can not only objectively reflect the working process of the MTS, but also fully characterize the complex correlation with shut down fault in the MTS. The qualitative assessment results of the system show that the cut set probability of the pantograph is the highest, which should be the focus of the MTS.
Association between NF‐κB Activation in Peripheral Blood Mononuclear Cells and Late Skin and Subcutaneous Fibrosis following Radiotherapy
Authors: Su Yapin , Zhang Yuyu , Sun Meiting , Liu Wenhui , Pei Fengli , Han Fujun
Published: July 2020

Background. This study aimed at evaluating the association between the speed of nuclear factor‐kappa B (NF‐κB) activation in peripheral blood mononuclear cells (PBMCs) and late skin and subcutaneous fibrosis in patients with head and neck squamous cell carcinoma (HNSCC) after radiotherapy. Methods. The speed of NF‐κB activation was represented by the nuclear p65 expression ratio before and after irradiation. The optimal time point to measure the ratio was determined by Western blot in the PBMCs from healthy outpatients ranging from 0 to 12 hours after ex vivo irradiation. We recruited patients with HNSCC who had received ratiotherapy and who were under regular follow‐up care. We assessed the association between the risk of developing ≥grade 2 late fibrosis and the nuclear p65 expression ratio in the PBMCs after ex vivo irradiation in these patients. Results. The maximum nuclear p65 ratio was observed at 1 hour after ex vivo irradiation in the PBMCs from the healthy outpatients. The speed of NF‐κB activation was then represented by the nuclear p65 ratio in the PBMCs before and 1 hour after ex vivo irradiation. A total of 200 patients with HNSCC were recruited, 32.50% (n = 65) of which presented with ≥grade 2 late fibrosis. There was a significant association between the speed of NF‐κB activation in the PBMCs and an increased risk of developing ≥grade 2 late fibrosis in these patients (P = 0.004). Subgroup analysis suggested that this finding was independent of the known clinical characteristics. Conclusions. The speed of NF‐κB activation might be a potential predictor of late toxicity in cancer patients after radiotherapy. Prospective studies are needed for validation.
Structure and Stability of Steady State Bifurcation in a Cannibalism Model with Cross-Diffusion
Authors: Chen Meijun , Fu Shengmao
Published: July 2020

This paper deals with spatial patterns of a predator-prey crossdiffusion model with cannibalism. By applying the asymptotic analysis and Rabinowitz bifurcation theorem, we consider the local structure of steady state to the model and determine an explicit formula of the nonconstant steady state. Furthermore, the criteria of the stability/instability for the steady state with small amplitude are established.
Thymoquinone Upregulates Catalase Gene Expression and Preserves the Structure of the Renal Cortex of Propylthiouracil-Induced Hypothyroid Rats
Authors: Ayuob Nasra , Balgoon Maha Jameal , El-Mansy Ahmed A. , Mubarak Wafaa A. , Firgany Alaa El-Din L.
Published: July 2020

Background. The association between hypothyroidism and renal diseases has been described in many studies. Nigella Sativa was among the recently reported natural product that has the potential to prevent renal tissue damage and fibrosis. The aim of this study was to evaluate the possible protective effect of thymoquinone on the structure of the renal cortex of hypothyroid rats and explore the mechanism behind it. Methods. An experimental model of hypothyroidism was induced in adult male Wistar rats by administration of propylthiouracil (6 mg/kg/body weight). One hypothyroid group was treated with thymoquinone at the dose of 50 mg/kg/body weight and compared to the untreated group. Thyroid function and oxidant/antioxidant status were assessed in the serum. Catalase gene expression was assessed using the real-time polymerase chain reaction. The kidney was assessed both histologically and immunohistochemically. Results. Administration of propylthiouracil resulted in a significant decrease in the serum levels of nitric oxide, reduced glutathione, and superoxide dismutase activity while the level of malondialdehyde significantly (p<0.001) increased. Administration of thymoquinone alleviated this effect on the thyroid hormones and significantly increased the serum levels of antioxidants. Thymoquinone significantly (p<0.001) upregulated catalase transcription by about 24-fold and could block the hypothyroidism-induced glomerular and tubular injury. Conclusion. Thymoquinone may have a potential protective effect against hypothyroidism-induced renal injury acting through the attenuation of the oxidative stress and upregulation of renal catalase gene expression.
Effect Analysis on Combustion and Emission Characteristics of a Rotary Burner Fueled by Biomass Pellet Fuel
Authors: Jia Guohai , Li Lijun , Zhang D. M.
Published: July 2020

A biomass pellet rotary burner was chosen as the research object, in order to study the influence of excess air coefficient on the combustion phenomenon of biomass rotary burner, the finite element simulation model of a biomass rotary burner was established, and simulation results of a biomass rotary burner were verified by the experiment. The computational fluid dynamics software was applied to simulate the combustion characteristics of biomass rotary burner in a steady-state condition, and the effect of excess air coefficient on temperature field and component concentration field in biomass rotary burner was analyzed. The results show that the flue gas flow rate inside the burner gradually increases with the increase of air velocity, the area with large temperature is mainly concentrated in the middle region of the rotary burner, and the maximum combustion temperature also appeared in the middle region of the combustion chamber, and the formation area of CO decreases with the increase of excess air coefficient. CO2 is mainly concentrated in the middle region of the burner, and the CO2 generating region decreases with the increase of excess air coefficient. The experimental value of the combustion temperature of the biomass rotary burner is in good agreement with the simulation results.
Rare Presentation of Cardiotoxicity Related to 5-Fluorouracil
Authors: Charkviani Mariam , Murvelashvili Natia , Barrera Francisco , Sharma Alisha , Eldin Randa Sharag , Nabil Nur Un Nisa
Published: July 2020

5-Fluorouracil (5-FU) is a chemotherapeutic agent frequently used for the treatment of solid tumors. In a few cases, 5-FU can be associated with coronary vasospasm, cardiac ischemia, or life-threatening arrhythmias. Recognition of 5-FU cardiotoxicity is clinically important as after the rapid sensation of therapy, cardiotoxicity can be completely reversible, and on the other hand, readministration may lead to serious damage of the heart and even death. A 70-year-old male came to the emergency department (ED) with chest pain which started while receiving an infusion of 5-FU. The patient did not have a personal history or risk factors of coronary artery disease and his electrocardiogram (ECG) before starting chemotherapy was completely normal. In the ED, his ECG had ischemic changes, troponin was elevated, and echocardiogram showed anterior wall hypokinesis. However, emergent coronary angiogram did not reveal any acute coronary occlusion. 5-FU-induced cardiotoxicity was suspected; the patient was admitted to a progressive care unit for close monitoring and infusion of calcium channel blockers was initiated. The patient’s symptoms and ECG findings gradually resolved, and two days later on discharge, patient was chest pain free and ECG was normal. This case supports the vasospastic hypothesis of 5-FU cardiac toxicity, describes its clinical course, and emphasizes the importance of better awareness and early recognition of the rare side effect as it may allow physicians to reduce the risk of life-threatening complications.
Health Education to Reduce Helminthiasis: Deficits in Diets in Children and Achievement of Students of Elementary Schools at Pontianak, West Kalimantan
Authors: Puspita Widyana Lakshmi , Khayan Khayan , Hariyadi Didik , Anwar Taufik , Wardoyo Slamet , Ihsan Bagus Muhammad
Published: July 2020

Worms are still a serious problem for poor and developing countries. Children, especially school-age children, are more at risk of infection. Efforts need to be made to prevent the effects of worms. Prevention can be done through a promotive approach. This observational study with a pre-posttest and cross-sectional approach is aimed at evaluating the effectiveness of health education on healthy and helminthic behavior and analyzing the impact of helminthiasis on the deficit in the diet and children’s learning achievement. The number of samples is 60 students from five grade 3 and 4 elementary schools in North Pontianak, West Kalimantan. The sampling technique was carried out by proportional random sampling. Worm infection in elementary school students was 16.7%, anemia was 55%, and learning achievement scores were less than the average grade of 55%. There was a significant difference in health counseling towards a decrease in the worm number (p=0.046). There was a significant relationship between healthy living behavior and helminthiasis (p=0.005). There was a significant relationship between helminthiasis and anemia (p=0.017). There is a relationship between helminthiasis and learning achievement in elementary school children (p=0.017). There is a relationship between anemia and learning achievement (p=0.005). It is necessary for public health centers to provide treatment services for worms and health education about the effects of helminthiasis on health and learning achievement. The school should provide hand washing facilities in schools, and parents should play an active role in improving clean and healthy lifestyle habits at home.
Pituitary Metastatic Composite Tumors: A Case Report with Next-Generation Sequencing and Review of the Literature
Authors: Helton Matthew , Abu-Rmaileh Muhammad , Thomas Kevin , Gokden Murat , Kanaan Alissa , Rodriguez Analiz
Published: July 2020

Background. While pituitary tumors are well understood, little research has been done on metastasis from primary tumors into pituitary adenomas, also known as composite tumors. Because only 34 cases of composite tumors have been reported to date, we hope to better characterize these tumors by reviewing cases reported in the literature and reviewed our own documented case, which includes next-generation sequencing. Case Presentation. A 74-year-old man presented to the emergency department with left vision loss for 3 months. He had a history of colon cancer treated with colectomy and clear cell renal carcinoma treated with left nephrectomy. A preoperative MRI demonstrated growth of a peripherally enhancing, centrally necrotic mass with sellar expansion measuring 5.7×3.1×3.0 cm. Given these findings, an endoscopic endonasal transsphenoidal resection was performed. Histological assessment revealed a composite tumor: one neoplasm was a nonfunctioning pituitary adenoma, and another neoplasm was a clear cell carcinoma. Next-generation sequencing demonstrated that the tumors shared mutations in VHL and Notch2. The patient died 2 months later from systemic metastatic cancer. Conclusion. From our literature review, most metastatic lesions in these composite tumors originated from neoplasms of the lung and kidney. Approximately 63% patients presented with ophthalmoplegia as the initial symptom while 23% displayed hormonal abnormalities. Postoperative mortality had a median of 3.5 months. In our patient, the presence of VHL and Notch2 mutations in both tumors highlights the possibility of using next-generation sequencing to help identify therapeutic targets even in complex composite neoplasms.
Composition Formulae for thek-Fractional Calculus Operators Associated withk-Wright Function
Authors: Suthar D. L.
Published: July 2020

In this article, thek-fractional-order integral and derivative operators including thek-hypergeometric function in the kernel are used for thek-Wright function; the results are presented for thek-Wright function. Also, some of special cases related to fractional calculus operators andk-Wright function are considered.
Fault Diagnosis of Rotating Machinery Based on Convolutional Neural Network and Singular Value Decomposition
Authors: Liu Dong , Lai Xu , Xiao Zhihuai , Liu Dong , Hu Xiao , Zhang Pei
Published: July 2020

Vibration signal and shaft orbit are important features that reflect the operating state of rotating machinery. Fault diagnosis and feature extraction are critical to ensure the safety and reliable operation of rotating machinery. A novel method of fault diagnosis based on convolutional neural network (CNN), discrete wavelet transform (DWT), and singular value decomposition (SVD) is proposed in this paper. CNN is used to extract features of shaft orbit images, DWT is used to transform the denoised swing signal of rotating machinery, and the wavelet decomposition coefficients of each branch of the signal are obtained by the transformation. The SVD input matrix is formed after single branch reconstruction of the different branch coefficients, and the singular value is extracted to obtain the feature vector. The features extracted from both methods are combined and then classified by support vector machines (SVMs). The comparison results show that this hybrid method has a higher recognition rate than other methods.
Moisture Migration and Control of New Embankment for Reconstruction and Expansion Project in Southern China
Authors: Zhang Junhui , Li Feng , Zeng Ling , Peng Junhui , Ding Le , He Liang
Published: July 2020

In hot and humid regions of southern China, the volumetric moisture content of the embankment after opening to traffic for a period of time reaches a stable state, and it is higher than the design value. When it was widened, the humidity gradient and exchange were formed due to the difference in moisture content between the existing and new embankment. To reveal the moisture migration of the existing and new embankment and control the rise of volumetric moisture content in new embankment, six frequency domain reflectometry sensors were installed in existing and new embankment to monitor the volumetric moisture content. A finite element model for the embankment was established and verified with the measured data. And seven numerical analyses of transient seepage in the new embankment of the cushion, cover, and partition using capillary barrier by sand were simulated. The results show that the volumetric moisture contents of the new embankment in southern China gradually increase and eventually reach an equilibrium state. The increase in water comes from the slope, the foundation, and the existing embankment. Early in the first 1∼2 years, the water mainly comes from the foundation and the existing embankment. After that, as time goes by, the water comes mostly from the slope infiltration and gradually migrates to the foundation and the existing embankment. Finally, the volumetric moisture content and the water storage gradually reach equilibrium. The volumetric moisture content of the new embankment using capillary barrier by sand at the cushion, the cover, and the partition is maintained as the construction volumetric moisture content. This combination is a very effective method to control the humidity stability of the new embankment in southern China.
Can Clinical Measures of Postoperative Binocular Function Predict the Long-Term Stability of Postoperative Alignment in Intermittent Exotropia?
Authors: Wu Yidong , Xu Meiping , Zhang Junxiao , Zhou Jinjing , Wan Minghui , Dai Zhiyue , Peng Tingting , Min Seung Hyun , Hou Fang , Zhou Jiawei , Yu Xinping
Published: July 2020

Purpose. To evaluate whether clinical measures of postoperative binocular functions could predict the long-term stability of postoperative ocular alignment in children with intermittent exotropia.Methods. A retrospective study was performed in thirty-nine children (median: 7 years) who have been surgically treated from intermittent exotropia without overcorrection (less than 10 prism diopters [pd] of exodeviation at 1 month postoperatively). Angles of deviation and binocular functions were measured preoperatively and at 1 month, 6 months, and the final follow-up visit (≥24 months) postoperatively. We examined the relationships between postoperative drift (change of ocular alignment) and binocular functions (sensory fusion, fusional convergence amplitude, and stereoacuity).Results. The surgical success rate (esophoria/tropia ≤5 pd to exophoria/tropia ≤10 pd) dropped to 76.9% at 6 months after surgery and to 53.8% at individuals’ last visit (mean: 37 months). The mean exodrift was 7.7 ± 9.2 pd from the postoperative month 1 to the final visit (p<0.001) on distance fixation. Distance stereoacuity, central fusion, and fusional convergence amplitude significantly improved following surgery (p<0.05). However, no significant correlation was found between their binocular functions measured at the beginning of each follow-up period and the postoperative drift (allp>0.13).Conclusion. Our findings suggest that the clinical measures of sensory fusion, fusional convergence amplitude, and stereoacuity cannot serve as a robust predictor for the long-term stability of postoperative ocular alignment in patients who underwent successful surgery without overcorrection at 1 month postoperatively.
Condition-Based Maintenance Optimization for Motorized Spindles Integrating Proportional Hazard Model with SPC Charts
Authors: Du Xuejiao , Gai Jingbo , Chen Cen
Published: July 2020

Reliability of motorized spindles has a great effect on the performance and productivity of computer numerical control (CNC) machine tools for intelligent manufacturing. Condition-based maintenance (CBM) is an efficient method to prevent serious failures, to improve system reliability, and to reduce management costs for motorized spindles. However, owing to various degradation features acquired during condition monitoring, the challenge is to propose an appropriate feature to evaluate the reliability level of motorized spindles and to set up optimal CBM policies. Based on the motivation, a three-stage approach is proposed in this paper. In the first stage, proportional hazard model (PHM) is developed to describe the reliability considering failure events together with multiple degradation features. Next, statistical process control (SPC) charts are constructed for condition monitoring and anomaly detection in order to achieve early detection of potential failures. At last, a CBM schedule is modeled in consideration of maintenance cost minimization; the maintenance plan is optimized by determining the optimal control limits of SPC charts.
A Time-Delay-Bounded Data Scheduling Algorithm for Delay Reduction in Distributed Networked Control Systems
Authors: Fan Zeming , Yu Xiaojun , Wan Hao , Kang Meilin , Liu Yuan , He Yuye , Xiao Gaoxi
Published: July 2020

As a key feature of networked control systems (NCSs), the time delays induced by communication medium sharing and data exchange over the system components could largely degrade the NCS performances or may even cause system instability, and thus, it is of critical importance to reduce time delays within NCSs. This paper studies the time-delay reduction problem in distributed NCSs and presents a dual-way data scheduling mechanism for time-delay reductions in delay-bounded NCSs with time-varying delays. We assess the time delays and their influences on the NCSs first with various delay factors being considered and then describe a one-way scheduling mechanism for network-delay reductions in NCSs. Based upon such a method, a dual-way scheduling algorithm is finally proposed for distributed NCSs with different types of transmitted data packets. Experiments are conducted on a remote teaching platform to verify the effectiveness of the proposed dual-way scheduling mechanism. Results demonstrate that, with the stability time-delay bound considered within the scheduling process, the proposed mechanism is effective for NCS time-delay reductions while addressing the stability, control accuracy, and settling time issues efficiently. Such a proposed mechanism could also be implemented together with some other existing control algorithms for time-delay reductions in NCSs. Our work could provide both useful theoretical guidance and application references for stable tracking control of delay-bounded NCSs.
Adaptive Cruise Control Strategy Design with Optimized Active Braking Control Algorithm
Authors: Wu Wenguang , Zou Debiao , Ou Jian , Hu Lin
Published: July 2020

The braking quality is considered as the most important performance of the adaptive control system that influences the vehicle safety and ride comfort remarkably. This research is aimed at designing an adaptive cruise control (ACC) system based on active braking algorithm using hierarchical control. Taking into account the vehicle with safety and comfort, the upper decision-making controller is designed based on model predictive control algorithm. Throttle controller and braking controller are designed with feedforward and feedback algorithms as the bottom controller, where the braking controller is designed based on the hydraulic braking model. The whole model is simulated collaboratively with Amesim, Carsim, and Simulink. By comparison with the full deceleration model, the results show that the proposed algorithm can not only make the vehicle maintain a safe distance under the premise of following the target vehicle ahead effectively but also provide favorable driving comfort.
Differential Gene Expression Responding to Low Phosphate Stress in Leaves and Roots of Maize by cDNA‐SRAP
Authors: Yan Lei , Su Liang , Li Rui , Li Hao , Bai Jianrong , Sun Fengjie
Published: July 2020

Phosphate (Pi) deficiency in soil can have severe impacts on the growth, development, and production of maize worldwide. In this study, a cDNA‐sequence‐related amplified polymorphism (cDNA‐SRAP) transcript profiling technique was used to evaluate the gene expression in leaves and roots of maize under Pi stress for seven days. A total of 2494 differentially expressed fragments (DEFs) were identified in response to Pi starvation with 1202 and 1292 DEFs in leaves and roots, respectively, using a total of 60 primer pairs in the cDNA‐SRAP analysis. These DEFs were categorized into 13 differential gene expression patterns. Results of sequencing and functional analysis showed that 63 DEFs (33 in leaves and 30 in roots) were annotated to a total of 54 genes involved in diverse groups of biological pathways, including metabolism, photosynthesis, signal transduction, transcription, transport, cellular processes, genetic information, and organismal system. This study demonstrated that (1) the cDNA‐SRAP transcriptomic profiling technique is a powerful method to analyze differential gene expression in maize showing advantageous features among several transcriptomic methods; (2) maize undergoes a complex adaptive process in response to low Pi stress; and (3) a total of seven differentially expressed genes were identified in response to low Pi stress in leaves or roots of maize and could be used in the genetic modification of maize.
Overexpression of MicroRNA‐16 Alleviates Atherosclerosis by Inhibition of Inflammatory Pathways
Authors: Wang Manman , Li Jiao , Cai Jiageng , Cheng Lijun , Wang Xuewen , Xu Pengjuan , Li Guangping , Liang Xue
Published: July 2020

Background. Our previous study demonstrated that the expression of miR‐16 was downregulated in the cell and animal models of atherosclerosis (AS), a main contributor to coronary artery disease (CAD). Overexpression of miR‐16 inhibited the formation of foam cells by exerting anti‐inflammatory roles. These findings indicated miR‐16 may be an anti‐atherogenic and CAD miRNA. The goal of this study was to further validate the expression of miR‐16 in CAD patients and explore its therapeutic roles in an AS animal model. Methods. A total of 40 CAD patients and 40 non‐CAD people were prospectively registered in our study. The AS model was established in ApoE‐/‐ mice fed a high‐fat diet. The model mice were randomly treated with miR‐16 agomiR (n = 10) or miR‐negative control (n = 10). Hematoxylin‐eosin staining was conducted for histopathological examination in thoracic aorta samples. ELISA and immunohistochemistry were performed to determine the expression levels of inflammatory factors (IL‐6, TNF‐α, MCP‐1, IL‐1β, IL‐10, and TGF‐β). qRT‐PCR and western blotting were carried out to detect the mRNA and protein expression levels of PDCD4, miR‐16, and mitogen‐activated protein kinase pathway‐related genes. Results. Compared with the normal control, miR‐16 was downregulated in the plasma and peripheral blood mononuclear cell of CAD patients, and its expression level was negatively associated with IL‐6 and the severity of CAD evaluated by the Gensini score, but positively related with IL‐10. Injection of miR‐16 agomiR in ApoE‐/‐ mice reduced the formation of atherosclerotic plaque and suppressed the accumulation of proinflammatory factors (IL‐6, TNF‐α, MCP‐1, and IL‐1β) in the plasma and tissues but promoted the secretion of anti‐inflammatory factors (IL‐10 and TGF‐β). Mechanism analysis showed overexpression of miR‐16 might downregulate target mRNA PDCD4 and then activate p38 and ERK1/2, but inactivate the JNK pathway. Conclusions. Our findings suggest miR‐16 may be a potential diagnostic biomarker and therapeutic target for atherosclerotic CAD.
Successful Use of Negative Pressure Wound Therapy for Abdominal Wall Necrosis Caused by a Perforated Ascending Colon Using the ABThera System
Authors: Imaoka Kouki , Yano Takuya , Choda Yasuhiro , Oshita Ko , Tani Yuma , Kubota Tetsushi , Ishida Michihiro , Satoh Daisuke , Yoshimitsu Masanori , Nakano Kanyu , Harano Masao , Matsukawa Hiroyoshi , Idani Hitoshi , Shiozaki Shigehiro , Okajima Masazumi
Published: July 2020

Background. The practice of leaving the abdomen open after an emergency laparotomy has gained increasing popularity recently. Negative pressure wound therapy (NPWT) is known as an effective technique in the management of an open abdomen (OA). A new device, the ABThera™ Open Abdomen Negative Pressure Therapy System (KCI USA, San Antonio, TX, USA), was specifically designed to achieve a temporary abdominal closure (TAC) in the management of an OA. This study was aimed at presenting a successful experience of treating a case of abdominal wall necrosis caused by a perforated ascending colon using the ABThera System. Case Presentation. A 66-year-old man was admitted to our hospital with complaints of severe pain in the abdomen. On admission, abdominal contrast-enhanced computed tomography (CT) showed fluid collection, an air pocket in the subcutaneous fat layer of the abdominal wall, and edematous changes in the adipose tissue in the peritoneum and abdominal wall. Based on a diagnosis of peritonitis resulting from a perforated ascending colon, emergency surgery was performed. A right hemicolectomy, ileostomy construction, and debridement of the necrotic tissues were performed. However, necrotizing fasciitis rapidly spread; therefore, more necrotic tissue was debrided in a second operation. The abdominal wall defect was left open, and the ABThera System was used in the management of the OA; this device promoted wound healing. A reduction was observed in the size of the open wound with visible granulation tissue. The defect was finally covered with a mesh split-thickness skin graft and anterolateral thigh flap. Conclusions. In the management of a case of a massive wound with infection, it can be of great benefit to treat the wound with NPWT initially to decrease its size. The ABThera System could facilitate early and safe management of an OA by surgeons.
Analysis on Hover Control Performance of T- and Cross-Shaped Tail Fin of X-Wing Single-Bar Biplane Flapping Wing
Authors: Zhang Pingxia , Zhu Junru , Zhu Yongqiang
Published: July 2020

The current flapping wing adopts T-shaped or cross-shaped tail fin to adjust its flight posture. However, how the tail fin will affect the hover control is not very clear. So, the effects of the two types of tail on flight will be analyzed and compared by actual flight tests in this paper. Firstly, we proposed a new X-wing single-bar biplane flapping-wing mechanism with two pairs of wings. Thereafter, the overall structure, gearbox structure, tail, frame, and control system of the flapping wing were designed and analyzed. Secondly, the control mechanism of hover is analyzed to describe the effect of two-tail fin on posture control. Thirdly, the Beetle was used as the control unit to achieve a controllable flight of flapping wing. The MPU6050 electronic gyroscope was used to monitor the drone’s posture in real time, and the Bluetooth BLE4.0 wireless communication module was used to receive remote control instructions. At last, to verify the flight effect, two actual flapping wings were fabricated and flight experiments were conducted. The experiments show that the cross-shaped tail fin has a better controllable performance than the T-shaped tail fin. The flapping wing has a high lift-to-mass ratio and good maneuverability. The designed control system can achieve the controllable flight of the flapping wing.
Altered Regulatory B Cell Subsets in Children with Type 1 Diabetes Mellitus
Authors: El-Mokhtar Mohamed A. , Elsherbiny Nahla M. , Sayed Douaa , Raafat Duaa M. , Askar Eman , Hussein Almontaser , Abdel-Malek Mohamed A. Y. , Shalaby Amira M.
Published: July 2020

B regulatory cells (Breg) refer to characteristic subsets of B cells that generally exert anti-inflammatory functions and maintain peripheral tolerance mainly through their ability to secrete interleukin-10 (IL10). Dysregulation in the function of Breg cells was reported in several autoimmune diseases. However, the relation between Breg and children with type 1 diabetes (T1D) is poorly understood. Thus, this study is aimed at determining whether Breg cells play a role in T1D in children or not, so we hypothesized that an altered phenotype of B cell subsets is associated with T1D in children. Children with T1D (n=29) and control children with normal blood glucose levels (n=14) were recruited. The percentages of different circulating IL10-producing Breg subsets, including B10, immature transitional, and plasmablasts were determined using flow cytometry analysis. Furthermore, the association between different IL10-producing B cells and patient parameters was investigated. The percentage of circulating IL10+CD24hiCD27+(B10) and IL10+CD24hiCD38hi(immature transitional) subsets of Breg cells was significantly lower in T1D patients than in healthy controls. Moreover, these cells were also negatively correlated with fasting blood glucose and HbA1c levels. Breg cells did not correlate with autoantibody levels in the serum. These findings suggest that certain Breg subsets are numerically deficient in children with T1D. This alteration in frequency is associated with deficient islet function and glycemia. These findings suggest that Breg cells may be involved in the loss of auto-tolerance and consequent destruction of pancreatic cells and could, therefore, be a potential target for immunotherapy.
Epigenetic Modifications due to Environment, Ageing, Nutrition, and Endocrine Disrupting Chemicals and Their Effects on the Endocrine System
Authors: Plunk Elizabeth C. , Richards Sean M.
Published: July 2020

The epigenome of an individual can be altered by endogenous hormones, environment, age, diet, and exposure to endocrine disrupting chemicals (EDCs), and the effects of these modifications can be seen across generations. Epigenetic modifications to the genome can alter the phenotype of the individual without altering the DNA sequence itself. Epigenetic modifications include DNA methylation, histone modification, and aberrant microRNA (miRNA) expression; they begin during germ cell development and embryogenesis and continue until death. Hormone modulation occurs during the ageing process due to epigenetic modifications. Maternal overnutrition or undernutrition can affect the epigenome of the fetus, and the effects can be seen throughout life. Furthermore, maternal care during the childhood of the offspring can lead to different phenotypes seen in adulthood. Diseases controlled by the endocrine system, such as obesity and diabetes, as well as infertility in females can be associated with epigenetic changes. Not only can these phenotypes be seen in F1, but also some chemical effects can be passed through the germline and have effects transgenerationally, and the phenotypes are seen in F3. The following literature review expands upon these topics and discusses the state of the science related to epigenetic effects of age, diet, and EDCs on the endocrine system.
Awareness and Perceptions on Health Warning Labels on Cigarette Packs among Smokers: A Cross‐Sectional Study
Authors: Hnin Thet Thet , Shein Nang Naing Naing , Aye San Kyu Kyu
Published: July 2020

Backgrounds. Tobacco use is the leading preventable cause of premature deaths. Tobacco control remains a top priority, and health warning labels (HWLs) are one of the recommended methods. This study is aimed at examining the awareness and perceptions of HWLs on cigarette packs among smokers. Methods. A cross‐sectional study was conducted among 240 smokers who were randomly recruited from three townships in Mandalay in 2018. A face‐to‐face interview was done using a questionnaire. Multivariate logistic regression was used to analyse the data. Results. About half were 18‐40 years old; the majority were males (96.3%) and smokers (93.4%). Nearly all respondents noticed both pictorial warning and text messages, and about half could identify the current size of HWLs. Most of the smokers generally had positive perceptions and opinions on HWLS, and they strongly supported it. About 75% intended to reduce the number of cigarettes, and 18% were willing to quit within 6 months. Those who desired to reduce the number of cigarettes were more likely to quit within 6 months (aOR = 7.6, 95% CI 1.6‐35.9 and aOR = 19.6, 95% CI 13.0‐294.7 for those who had a little and strong desire, respectively). Conclusion. Awareness status and perceptions of the respondents were acceptable, and HWLs have motivated smokers to quit smoking. The Tobacco Control Program needs to strengthen the tobacco control law that prohibits selling loosies in order to maximize the benefits of HWLs.
Influence of Mobile Payment on Bus Boarding Service Time
Authors: Chen Guojun , Chen Weilun , Zhang Shuyang , Zhang Dong , Liu Haode
Published: July 2020

A new form of mobile payment, Quick Response (QR) code, has been a popular way of paying bus fares in China since 2017. Compared with conventional payment methods, cash or IC card, QR code shows a lot of differences in response time, recognition accuracy, and payment procedure, which significantly influences the boarding service time (BST) for passengers. However, no research has considered its efficiency. This study, therefore, tries to fill this gap and investigate its influence on BST. Sufficient ride-check data were collected, and the influence of the QR code payment method on BST was examined through a set of regression models. Passengers pay the bus fare with different payment methods as their first choice; nevertheless, when the payment fails, they may transfer among them. According to the payment choice, result, and process, we introduce the first-choice-based, the last-choice-based, and the choice-transfer-based models, respectively. The scenario with delays in calling out the QR code was considered in the choice-transfer-based model. The onboard crowdedness was regarded as a categorical variable to determine the regime of the boarding process in all models. We conduct empirical analysis in Wuhan, and this study can help to identify the influence of the QR code payment method on BST, consequently, improving bus service efficiency.
Loss of GFAP and Vimentin Does Not Affect Peri-Infarct Depolarizations after Focal Cerebral Ischemia
Authors: Zheng Zelong , Yi Xuxia , Lv Jianping
Published: July 2020

Peri-infarct depolarization (PID), one kind of spreading depolarization, contributes to infarct volume enlargement after ischemic stroke. Astrocytes participate in PIDs by various mechanisms. The roles of glial fibrillary acidic protein (GFAP) and vimentin (Vim), intermediate filament proteins in astrocytes, however, in PIDs induction and propagation remain unknown. Middle cerebral artery occlusion (MCAO) model was made in 9 GFAP−/−Vim−/− and 9 wild-type (WT) C57BL/6 mice. Using 4-wavelength optical intrinsic signal imaging (OIS), we identified PIDs as consistent, red and blue interaction waves in the cortical reflectance that slowly propagated peripherally from the origin site. Five propagation patterns of PIDs were observed after MCAO in mice, namely, latero-medial, medial-lateral, rostro-caudal, caudo-rostral, and collision. Additionally, the frequency, propagation velocity, and duration of PIDs between GFAP−/−Vim−/− and WT mice were not significantly different (p > 0.05). Furthermore, no significant difference was found in infarct volume and brain edema between the two groups. In conclusion, the 4-wavelength OIS system allows acquisition of high temporal-spatial resolution color images for analyzing temporal-spatial characteristics of PIDs in detail. GFAP and Vim in astrocytes are not involved in PIDs after MCAO in mice.
Patient Characteristics and Outcomes of Relapsed/Refractory Multiple Myeloma in Patients Treated with Proteasome Inhibitors in Germany
Authors: Steinmetz H. Tilman , Singh Moushmi , Lebioda Andrea , Gonzalez-McQuire Sebastian , Rieth Achim , Schoehl Martina , Poenisch Wolfram
Published: July 2020

Introduction: Real-world data reflects treatments and outcomes in clinical practice in contrast with controlled clinical trials. This study evaluates real-life multiple myeloma (MM) patients receiving proteasome inhibitor (PI)-based treatments in the second or third therapy line in 2017 in Germany. Methods: This is a retrospective chart review on adult relapsed/refractory MM patients treated with ≥1 dose of a PI-based regimen in either the second or the third line of therapy. Participating physicians had ≥3 years of clinical experience in treating symptomatic MM patients and used PI according to the label. Results: Distinct patient profiles for each PI-based regimen emerged. Younger, fitter, transplant-eligible patients received novel PI triplets such as carfilzomib in combination with lenalidomide and dexamethasone (KRd) or IRd. Patients receiving lenalidomide in first-line therapy mostly received lenalidomide-free regimens in second-line therapy. In high-risk patients, no clear treatment patterns could be ascertained. The complete response rates were highest with KRd (13.0%), followed by carfilzomib in combination with dexamethasone (Kd) (5.7%) and bortezomib (4.8%). The very good partial response rates were highest with IRd (76.9%), followed by KRd (53.7%), Kd (25.7%), and bortezomib (20.5%). None of the KRd- or IRd-treated patients responded below a partial response. Discussion/Conclusion: Clear patient profiles for each PI type were observed. In second-line therapy, younger, fitter, transplant-eligible patients received novel-PI-based triplets, e.g., KRd or IRd. Patients treated with lenalidomide in first-line therapy mostly received lenalidomide-sparing regimens in second-line therapy. In high-risk patients no clear treatment patterns could be ascertained due to the limited sample size.
Higher Eosinophils Predict Death-Censored Technique Failure in Peritoneal Dialysis Patients
Authors: Yang Jiayi , Fan Jinjin , Fan Li , Yi Chunyan , Lin Jianxiong , Mao Haiping , Yang Xiao , Wang Xin
Published: July 2020

Introduction: Eosinophilia (eosinophil fraction of leukocytes >5%), an indicative parameter for bioincompatibility in various circumstances, is well established in hemodialysis. However, change in eosinophil count (EOC) and its association with death-censored technique failure among peritoneal dialysis (PD) patients remain unclear. Methods: We compared eosinophils before and after PD initiation among 1,432 eligible continuous ambulatory PD patients regularly followed up in our PD center during 2007–2018. Risk factors of early-stage eosinophilia were examined by the logistic regression test. The relationship of early-stage eosinophilia and EOC with death-censored technique failure was examined using the Cox proportional hazards model for overall patients and for men and women separately. Results: After PD initiation, the EOC and percentage of patients with eosinophilia were significantly increased compared with baseline. Being male (odds ratio [OR]: 2.26; 95% confidence interval [CI]: 1.55–3.31; p < 0.001) and higher EOC at baseline (100 cells/μL increase, OR: 1.62; 95% CI: 1.45–1.82; p < 0.001) were risk factors of early-stage eosinophilia after PD initiation. During follow-up, 204 death-censored technique failures were recorded. In fully adjusted models, each with 100 cells/μL increase in EOC, the adjusted hazard ratios (HRs) of technique failure were 1.11 (95% CI: 1.03–1.20; p = 0.009) in the whole cohort, 1.29 (95% CI: 1.10–1.51; p = 0.002) in women, and 1.07 (95% CI: 0.97–1.17; p = 0.196) in men. Eosinophilia was significantly associated with the risk of technique failure for women (HR: 2.24; 95% CI: 1.07–4.70; p = 0.033), which was especially significant for women aged <55 years (HR: 7.61; 95% CI: 1.88–30.90; p = 0.005). Conclusion: EOC was increased significantly after PD initiation, and increased numbers of eosinophils were associated with higher death-censored technique failure in PD patients, especially women.
LH Supplementation in Ovarian Stimulation for IVF: The Individual, LH Deficient, Patient Perspective
Authors: Kol Shahar
Published: July 2020

The availability of recombinant follicle-stimulating hormone (FSH) and luteinizing hormone (LH) opens an opportunity to individualize ovarian stimulation. While the need for FSH in ovarian stimulation is universal, a question remains whether exogenous LH is beneficial. Previous population-based research showed that added LH is indicated in elderly and in profoundly LH depressed patients. This commentary explores potential individual patient parameters that may hint that this specific individual may prospectively need supplemented LH, irrespective of her age or experience from previous cycles. Specifically, it is suggested that in an antagonist protocol, the degree of LH recovery 24 h post first GnRH antagonist injection can identify those patients who may benefit from added LH. In addition, rising LH during the first 5 days of stimulation may predispose patients to a sharp LH drop following the first GnRH antagonist dose, and the need for added LH.