bluuebunny/crossref_metadata_2025 huggingface dataset:
Abnormal degree centrality values as a potential imaging biomarker for major depressive disorder: A resting-state functional magnetic resonance imaging study and support vector machine analysis
Authors: Lin Hang , Xiang Xi , Huang Junli , Xiong Shihong , Ren Hongwei , Gao Yujun
Published: September 2022

Objective

Previous studies have revealed abnormal degree centrality (DC) in the structural and functional networks in the brains of patients with major depressive disorder (MDD). There are no existing reports on the DC analysis method combined with the support vector machine (SVM) to distinguish patients with MDD from healthy controls (HCs). Here, the researchers elucidated the variations in DC values in brain regions of MDD patients and provided imaging bases for clinical diagnosis.

Methods

Patients with MDD (N = 198) and HCs (n = 234) were scanned using resting-state functional magnetic resonance imaging (rs-fMRI). DC and SVM were applied to analyze imaging data.

Results

Compared with HCs, MDD patients displayed elevated DC values in the vermis, left anterior cerebellar lobe, hippocampus, and caudate, and depreciated DC values in the left posterior cerebellar lobe, left insula, and right caudate. As per the results of the SVM analysis, DC values in the left anterior cerebellar lobe and right caudate could distinguish MDD from HCs with accuracy, sensitivity, and specificity of 87.71% (353/432), 84.85% (168/198), and 79.06% (185/234), respectively. Our analysis did not reveal any significant correlation among the DC value and the disease duration or symptom severity in patients with MDD.

Conclusion

Our study demonstrated abnormal DC patterns in patients with MDD. Aberrant DC values in the left anterior cerebellar lobe and right caudate could be presented as potential imaging biomarkers for the diagnosis of MDD.
An investigation of the incidence of post-traumatic stress disorder, turnover intention and psychological resilience among medical staff in a public hospital in China during the outbreak of the omicron variant in the COVID-19 pandemic in 2022
Authors: Jing Cui , Feng-Hong Zhang , Yi-Yan Wang
Published: September 2022

Objective

To investigate the incidence of post-traumatic stress disorder (PTSD), turnover intention and psychological resilience of medical staff during the Outbreak of the Omicron Variant in the COVID-19 pandemic in 2022 and to provide a basis for adopting relevant psychological interventions to reduce medical staff turnover.

Methods

Using the PTSD Checklist-Civilian Version (PCL-C) and a total score ranging from 17 to 85 points, a total score ≥ 38 indicates significant PTSD symptoms and a diagnosis of PTSD. The Chinese version of the Turnover Intention Scale (TIS) has a total score of 6 to 24 points; the higher the score, the stronger the turnover intention. The Chinese version of the Connor-Davidson Resilience Scale (CD-RISC) has a total score of 0 to 100 points, with higher scores indicating a better level of psychological resilience. A total of 443 front-line medical staff working in Chinese public hospitals and still treating all patients normally during COVID-19 were invited via the internet to complete a survey from 15 May to 30 May 2022 in China.

Results

The incidence of PTSD was 14.4%, the total turnover intention score was 13.38 ± 4.08, and the total psychological resilience score was 87.16 ± 18.42. The prevalence of PTSD was higher among medical staff who were married, had children, and were worried about being infected; in addition, the PTSD group had a higher level of education, higher turnover intention, and lower psychological resilience than the non-PTSD group. The total scores for turnover intention and fear of being infected were risk factors for PTSD, while a high total psychological resilience score and high education level were protective factors for PTSD; the differences were statistically significant (all P < 0.05).

Conclusion

Post-traumatic stress disorder among Chinese medical personnel was associated with the marital status, childbirth, education level, turnover intention, and psychological resilience. Among these factors, psychological resilience might be exploited as a protective factor.
Exploring seasonal diurnal surface temperature variation in cities based on ECOSTRESS data: A local climate zone perspective
Authors: Shi Zhipeng , Yang Jun , Wang Ling-en , Lv Fang , Wang Guiyang , Xiao Xiangming , Xia Jianhong
Published: September 2022

High urban temperatures affect city livability and may be harmful for inhabitants. Analyzing spatial and temporal differences in surface temperature and the thermal impact of urban morphological heterogeneity can promote strategies to improve the insulation of the urban thermal environment. Therefore, we analyzed the diurnal variation of land surface temperature (LST) and seasonal differences in the Fifth Ring Road area of Beijing from the perspective of the Local Climate Zone (LCZ) using latest ECOSTRESS data. We used ECOSTRESS LST data with a resolution of 70 m to accurately interpret the effects of urban morphology on the local climate. The study area was dominated by the LCZ9 type (sparse low-rise buildings) and natural LCZ types, such as LCZA/B (woodland), LCZD (grassland), and LCZG (water body), mainly including park landscapes. There were significant differences in LST observed in different seasons as well as day and night. During daytime, LST was ranked as follows: summer > spring > autumn > winter. During night-time, it was ranked as follows: summer > autumn > spring > winter. All data indicated that the highest and lowest LST was observed in summer and winter, respectively. LST was consistent with LCZ in terms of spatial distribution. Overall, the LST of each LCZ during daytime was higher than that of night-time during different seasons (except winter), and the average LST of each LCZ during the diurnal period in summer was higher than that of other seasons. The LST of each LCZ during daytime in winter was lower than that of the corresponding night-time, which indicates that it is colder in the daytime during winter. The results presented herein can facilitate improved analysis of spatial and temporal differences in surface temperature in urban areas, leading to the development of strategies aimed at improving livability and public health in cities.
SLOfit Lifelong: A model for leveraging citizen science to promote and maintain physical fitness and physical literacy across the lifespan
Authors: Jurak Gregor , Morrison Shawnda A. , Soric Maroje , Leskošek Bojan , Kovač Marjeta , Ocvirk Tjaša , Sember Vedrana , Kramaršič Jaka , Meh Kaja , Potočnik Žan Luca , Blagus Rok , Markelj Neja , Golja Petra , Strojnik Vojko , Hadžić Vedran , Starc Gregor
Published: September 2022

SLOfit Lifelong is a public health initiative which was created to upgrade a well-established, national physical fitness surveillance system for Slovenian schoolchildren that has been collecting annual fitness and health data for over three decades. The ultimate objective of creating SLOfit Lifelong was to build a modern societal infrastructure with the capacity and ability to detect future causal associations between childhood physical fitness trends and future health outcomes based on the lifelong surveillance of one's own fitness status. By instilling citizens with an ambition to test, understand, and follow-up their own physical fitness and health status (including related health risk factors), this initiative provides the technical support and expert feedback needed to engender greater individual control over understanding (and thus modulating), one's own physical fitness status as they progress into older adulthood. This perspective paper details the extensive approach taken to devise appropriate fitness test batteries for adults and older adults which can also relate to the student version of the original SLOfit test database, including establishing criterion health risk zones and a public approach to establish this national, citizen-driven health feedback framework. Through its sophisticated online web applications, social media, print media, and outreach workshops, SLOfit Lifelong provides the expert support for public health engagement by fostering positive lifelong physical literacy experiences an individual can enjoy across their aging journey.
Factors associated with self-perceived health status in Portugal: Results from the National Health Survey 2014
Authors: Shaaban Ahmed Nabil , Martins Maria Rosario O. , Peleteiro Bárbara
Published: September 2022

Background

Self-perceived health is an important indicator of illness and mortality. This study aims at identifying a wide range of factors that can influence self-perceived health status among a representative sample in Portugal.

Methods

We used the 2014 National Health Survey (n = 17,057), whereby participants were required to assess their health status from “Very good,” “Good,” “Fair,” “Poor” to “Very poor.” We grouped the answers “Very good” and “Good,” and “Poor” and “Very poor,” respectively. Multinomial logistic regression was used to compare participants' characteristics across groups by computing odds ratio and corresponding 95% confidence intervals. Models included Socioeconomic/demographic characteristics, objective health status, healthcare use, functional disability, barriers to healthcare services utilization, lifestyle variables, mental health status, social support, and satisfaction with life as potential factors that can affect self-perceived health. Models were adjusted for sex, age, educational level, degree of urbanization, and presence of chronic diseases.

Results

About 45% of participants reported good/very good, 39% reported fair, while ~16% reported poor/very poor health perception. Poor/very poor health was more reported by women when compared to men (19.1 vs. 11.4%, respectively, p < 0.001). A higher prevalence of poor/very poor health status was reported by participants living in thinly populated areas or among older populations. Lower educational levels, lower income, as well as unemployment, were found to increase the risk of reporting poor/very poor health status. Utilizing healthcare services more frequently, experiencing barriers to access healthcare services, having depressive symptoms or activity limitations, or lacking social support were found to be significantly associated with poor/very poor self-perceived health.

Conclusion

Subjects living in Portugal tend to report less good/very good health status and more poor/very poor health when compared to the rest of Europe. This study stresses the importance of socioeconomic factors, chronic illness, barriers to access healthcare services, social isolation, and mental health status in influencing self-perceived health and highlights the urgent need for social-informed policies, strategies, and interventions to reduce health inequalities in Portugal.
The systematic analysis and 10-year prediction on disease burden of childhood cancer in China
Authors: Zhu Bo , Wu Xiaomei , An Wenxiu , Yao Bing , Liu Yefu
Published: September 2022

Background

There is a lack of in-depth analysis regarding the disease burden of childhood cancer in China. Indeed, this is the first time the topic has been addressed in detail. Drawing on population-based data for the past 30 years, this study systematically analyzes the composition and long-term trend of this disease burden in China.

Methods

GBD 2019 contained population-based data from 1990 to 2019 and was prepared using Microsoft Excel 2016. We used AAPC and ARIMA models for trend analysis and prediction formulation.

Results

In 2019, there were 45,601 new cases, 9,156 cancer deaths, and 782,530 DALYs in China. From 1990 to 2019, leukemia, together with brain and CNS cancer, invariably ranked highest in terms of new cases, cancer deaths, and DALYs. Leukemia accounted for more than 50%, but decreased over time. By contrast, the proportions for brain and CNS cancer increased. There were significant decreases in the overall incidence, mortality, and DALY rates in China, but these were still higher than the corresponding global average levels. Considering all types of childhood cancer, the incidence rate of testicular cancer showed the biggest increase, and the mortality and DALY rates of leukemia showed the largest decrease. In terms of different age groups, the overall incidence rate of childhood cancers increased in 0 to 4 age group, but it decreased in 5 to 14 age groups. The overall mortality and DALY rates of childhood cancers decreased in all four age groups. Over the next 10 years, the overall incidence rate of childhood cancer will increase, but the overall mortality and DALY rates will decrease. The increase in malignant skin melanoma will comprise the largest rise in the incidence, while the decrease for leukemia will be the largest fall in the incidence, cancer deaths, and DALYs.

Conclusion

The disease burden of all childhood cancers in China remains highly serious, especially for certain types of cancer and certain age groups. China should focus more emphatically on the incidence of childhood cancer in future, and it must consistently strengthen investment in the relevant research and medical resources to reduce the disease burden in this field.
Association between perceived partner support and lifestyle in mother-father dyads expecting a first child
Authors: Versele Vickà , Bogaerts Annick , Devlieger Roland , Matthys Christophe , Gucciardo Leonardo , Deliens Tom , Clarys Peter , Aerenhouts Dirk
Published: September 2022

Background

A healthy lifestyle during pregnancy is important for the health of mother and child. However, unfavorable physical activity (PA) and dietary changes are observed in pregnant women and their partner. Partner's influence on lifestyle has been reported by expectant women and men. The first aim was to analyze associations between perceived partner support on expectant parents own as well as their partner's moderate-to-vigorous intensity PA (MVPA) levels and dietary intake. Secondly, this study aimed to investigate intra-couple associations of MVPA, dietary intake and perceived support.

Methods

A total of 152 heterosexual couples were recruited between week 8 and 10 of gestation by means of convenience sampling. Objective anthropometric and 7-day MVPA measurements were performed at 12 weeks of gestation. An online questionnaire was used to assess dietary intake, perceived partner support and socio-demographic characteristics. An Actor-Partner Interdependence Model for distinguishable dyads was constructed to examine the relationship between perceived partner support and both own's (i.e., actor-effect) and the partner's (i.e., partner-effect) MVPA levels, intake of fruits and vegetables, and an “avoidance food group.”

Results

For pregnant women, perceived support from their partner was significantly associated with women's own MVPA levels (i.e., actor-effect; estimate = 0.344, SE = 0.168, p = 0.040) as well as the MVPA levels of the men (i.e., partner-effect; estimate = 0.717, SE = 0.255, p = 0.005). No significant actor- nor partner-effects were found for the expectant fathers. For none of the sexes significant actor-effects were found for fruit/vegetables and “avoidance food group” intake. For pregnant women, there was a positive partner-effect for fruit and vegetable intake (estimate = 7.822, SE = 1.842, p < 0.001) and a negative partner-effect for the “avoidance food group” intake (estimate = −16.115, SE = 3.629, p < 0.001). Positive correlations were found for perceived MVPA support (r = 0.40, p < 0.001), MVPA levels (r = 0.24, p = 0.007) and food intake from the “avoidance food group” (r = 0.28, p = 0.005) between partners.

Conclusion

This study shows that male partners can act as significant facilitators for women. Partners may be an important target when promoting MVPA during pregnancy. Additionally, supportive couples seem to strengthen each other in keeping a healthy lifestyle in early pregnancy. These results justify couple-based interventions in the promotion of a healthy lifestyle during the transition to parenthood.

Clinical trial registration

Clinicaltrials.gov, identifier: NCT03454958.
Risk attitudes and household consumption behavior: Evidence from China
Authors: Xie Xin , Tong Zefeng , Xu Shulin
Published: September 2022

Risk attitude is a vital component of public mental health. Thus, the public should be guided to fully comprehend risks to improve public mental health. Using panel data from China Household Finance Survey (CHFS) in 2017, this study examined the impact of risk attitudes on household consumption behavior by constructing a micro-econometric model. Results suggest that risk attitude can promote household consumption, with multiple robustness tests supporting this conclusion. In addition, after dividing the consumption types into subsistence consumption, development consumption, and enjoyment consumption, we show risk preference promotes all three types of consumption and has the greatest impact on enjoyment consumption. Concurrently, risk neutrality can promote household survival consumption, but its promotion effect is smaller than that of risk preference. Moreover, risk aversion has an inhibitory effect on total consumption behavior, but this inhibitory effect does not show heterogeneity for different consumption behaviors. Heterogeneity analysis found that for male households, risk attitude remains an important factor in consumption behavior. When men's risk attitude is more risk averse, it can promote more survival consumption, whereas women's risk attitude is more risk averse. With increasing age, risk attitude remains a crucial factor in the occurrence of consumer behavior. However, education level has no bearing on the effect of risk attitude on household consumption behavior. This research holds theoretical and practical significance for improving public mental health, optimizing residents' consumption structure, and achieving high-quality economic development.
Machine learning algorithms identifying the risk of new-onset ACS in patients with type 2 diabetes mellitus: A retrospective cohort study
Authors: Zhong Zuoquan , Sun Shiming , Weng Jingfan , Zhang Hanlin , Lin Hui , Sun Jing , Pan Miaohong , Guo Hangyuan , Chi Jufang
Published: September 2022

Background

In recent years, the prevalence of type 2 diabetes mellitus (T2DM) has increased annually. The major complication of T2DM is cardiovascular disease (CVD). CVD is the main cause of death in T2DM patients, particularly those with comorbid acute coronary syndrome (ACS). Although risk prediction models using multivariate logistic regression are available to assess the probability of new-onset ACS development in T2DM patients, none have been established using machine learning (ML).

Methods

Between January 2019 and January 2020, we enrolled 521 T2DM patients with new-onset ACS or no ACS from our institution's medical information recording system and divided them into a training dataset and a testing dataset. Seven ML algorithms were used to establish models to assess the probability of ACS coupled with 5-cross validation.

Results

We established a nomogram to assess the probability of newly diagnosed ACS in T2DM patients with an area under the curve (AUC) of 0.80 in the testing dataset and identified some key features: family history of CVD, history of smoking and drinking, aspartate aminotransferase level, age, neutrophil count, and Killip grade, which accelerated the development of ACS in patients with T2DM. The AUC values of the seven ML models were 0.70–0.96, and random forest model had the best performance (accuracy, 0.89; AUC, 0.96; recall, 0.83; precision, 0.91; F1 score, 0.87).

Conclusion

ML algorithms, especially random forest model (AUC, 0.961), had higher performance than conventional logistic regression (AUC, 0.801) for assessing new-onset ACS probability in T2DM patients with excellent clinical and diagnostic value.
Applying risk matrices for assessing the risk of psychosocial hazards at work
Authors: Taibi Yacine , Metzler Yannick A. , Bellingrath Silja , Neuhaus Ciel A. , Müller Andreas
Published: September 2022

Although wide-ranging amendments in health and safety regulations at the European and national level oblige employers to conduct psychosocial risk assessment, it is still under debate how psychosocial hazards can be properly evaluated. For psychosocial hazards, an epidemiological, risk-oriented understanding similar to physical hazards is still missing, why most existing approaches for hazard evaluation insufficiently conceive psychosocial risk as a combination of the probability of a hazard and the severity of its consequences (harm), as found in traditional risk matrix approaches (RMA). We aim to contribute to a methodological advancement in psychosocial risk assessment by adapting the RMA from physical onto psychosocial hazards. First, we compare and rate already existing procedures of psychosocial risk evaluation regarding their ability to reliably assess and prioritize risk. Second, we construct a theoretical framework that allows the risk matrix for assessing psychosocial risk. This is done by developing different categories of harm based on psychological theories of healthy work design and classifying hazards through statistical procedures. Taking methodological and theoretical considerations into account, we propose a 3 × 3 risk matrix that scales probability and severity for psychosocial risk assessment. Odds ratios between hazards and harm can be used to statistically assess psychosocial risks. This allows for both risk evaluation and prioritizing to further conduct risk-mitigation. Our contribution advances the RMA as a framework that allows for assessing the relation between psychosocial hazards and harm disregarding which theory of work stress is applied or which tool is used for hazard identification. By this, we also contribute to further possible developments in empirical research regarding how to assess the risk of workplace stress. The risk matrix can help to understand how psychosocial hazards can be evaluated and organizations can use the approach as a guidance to establish a suitable method for psychosocial risk evaluation.
Association between IFNGR1 gene polymorphisms and tuberculosis susceptibility: A meta-analysis
Authors: Cheng Liwei , Zhang Fan , Wang Ying , Chen Jing , Yuan Xiaoping
Published: September 2022

The association of IFN-γ receptor 1 (IFNGR1) gene polymorphisms with tuberculosis (TB) susceptibility has not been systematically studied. We therefore conducted a meta-analysis to assess their association. Literature search was conducted in PubMed, EMBASE, Web of Science, and the Cochrane Library. Odds ratio (OR) and 95% confidence interval (CI) was pooled by the random-effect model. Statistical analyses were performed using STATA 12.0 software. Fourteen studies involved 7,699 TB cases and 8,289 controls were included in this meta-analysis. A significant association was found between the IFNGR1 rs2234711 polymorphism and TB susceptibility among Africans in dominant model (OR = 1.24, 95%CI:1.01–1.52), and among Asians in allele model (OR = 0.89, 95%CI: 0.79–0.99), homozygote model (OR = 0.82, 95%CI: 0.70–0.98) and additive model (OR = 0.90, 95%CI: 0.83–0.97). In addition, a significant association was observed between the IFNGR1 rs7749390 polymorphism and TB susceptibility among Africans in allele model (OR = 0.89, 95%CI: 0.82–0.98). No significant association was found between the IFNGR1 rs1327474 polymorphism and TB susceptibility. In summary, IFNGR1 rs2234711 polymorphism was associated with increased TB susceptibility in Africans and decreased TB susceptibility in Asians, while IFNGR1 rs7749390 polymorphism was associated with decreased TB susceptibility in Africans.
Factors affecting social isolation among the young adults in South Korea: A cross-sectional analysis
Authors: Lee Soo-Bi , Shin Yerim , Jeon Yebin , Kim Seohyun
Published: September 2022

The recent increase in lonely deaths among young people has emphasized the emergence of social isolation as a social problem. This study investigated the issue of social isolation by examining multidimensional factors that affect social isolation and evaluating the subjective and objective dimensions of young adults. Data for this study were collected for 8 days (February 7–14, 2022) through an online questionnaire survey by a professional survey agency, based on proportional allocation of the young adult population (age 19–39 years); data from 521 young adults were analyzed. Regression analysis was performed using SPSS to identify multidimensional factors (relative deprivation, future outlook, depression, self-esteem, social skills, experience of violence, and online activity) that affect social isolation and showed that: (1) among sociodemographic characteristics, higher age and unemployment were associated with greater social isolation; (2) sociopsychological characteristics, such as higher levels of relative deprivation and depression, more negative future outlook, and lower self-esteem, all correlated with greater social isolation; and (3) among relationship characteristics, lower social skills and a stronger history of experiencing violence were linked to greater social isolation. These results highlight the need for a customized support system at the national level that takes into account the developmental tasks of young adults as a preventive strategy to solve the problem of social isolation of young adults and to devise various strategies to provide them with mental health services.
Bibliometric analysis of research trends and characteristics of drug-induced gingival overgrowth
Authors: Zhang Ruonan , Wu Jie , Zhu Junyi , Wang Xiaoxiao , Song Jiangyuan
Published: September 2022

Objectives

Drug-induced gingival overgrowth (DIGO) is a frequent adverse medication reaction that is generally caused by cyclosporine, phenytoin, and nifedipine, which belong to the category of immunosuppressants, anticonvulsants, and calcium channel blockers, respectively. This bibliometric analysis aims to depict the main citation characteristics and analyze the research trends in DIGO investigations.

Methods

An exhaustive search was performed in the Scopus database to create the bibliometric list of DIGO in the syntax. Furthermore, the information related to the number of citations, drugs related to DIGO, study topic and design, authorship, publication year, journal, contributing institution, country of origin, and the department was extracted.

Results

In total, 399 papers on DIGO were retrieved in this study. The total number of citations and that after the removal of self-citations were 7,814 and 7,314, respectively. The mean number of citations was 19.6 in a range of 0–608. The main paper types were articles (76.94%) and reviews (19.55%). A remarkable increasing trend in the number of citations has been observed since 1994. Cyclosporine (44.89%) is the most commonly used drug that shares a close relationship with DIGO, followed by phenytoin (18.22%), nifedipine (17.93%), and amlodipine (6.81%). The review (27.82%) type constituted the most widely used design in the DIGO studies. According to the top 20 keywords, the risk factors and pathogenesis of DIGO have been prominent topics of research works for several years.

Conclusions

This bibliometric analysis will facilitate the understanding of researchers and clinicians, especially those at the beginning of their careers in periodontology on DIGO, by identifying landmark research and providing an overview of this field.
Association of electroencephalogram epileptiform discharges during cardiac surgery with postoperative delirium: An observational study
Authors: Li Na , Liu Xing , Gao Yuhua , Yin Lingzi , Zhao Wanli , Ma Rongxing , Ni Xinli
Published: September 2022

Background

Delirium is a frequent and serious complication following cardiac surgery involving cardiopulmonary bypass (CPB). Electroencephalography reflects the electrical activity of the cerebral cortex. The impact of electroencephalographic epileptiform discharges during cardiac surgery on postoperative delirium remains unclear. This study was designed to investigate the relationship between intraoperative epileptiform discharges and postoperative delirium in patients undergoing cardiac surgery.

Methods

A total of 76 patients who underwent cardiac surgery under CPB were included. The baseline cognitive status was measured before surgery. Electroencephalograms were monitored continuously from entry into the operating room to the end of surgery. The presence of delirium was assessed through the Confusion Assessment Method or the Confusion Assessment Method for the Intensive Care Unit on the first 3 days after surgery. Univariate and multivariate logistic regression analyses were performed to evaluate the association between epileptiform discharges and delirium.

Results

Delirium occurred in 31% of patients and epileptiform discharges were present in 26% of patients in the study. Patients with delirium had a higher incidence of epileptiform discharges (52.63% vs. 13.95%, P < 0.001) and longer durations of anesthesia and CPB (P = 0.023 and P = 0.015, respectively). In addition, patients with delirium had a longer length of hospital stay and a higher incidence of postoperative complications. Multivariate logistic regression analysis showed that age and epileptiform discharges were significantly associated with the incidence of postoperative delirium [odds ratio, 4.75 (1.26–17.92), P = 0.022; 5.00 (1.34–18.74), P = 0.017, respectively].

Conclusions

Postoperative delirium is significantly related to the occurrence of epileptiform discharges during cardiac surgery.
Integrated approaches to howler monkey (Alouatta spp.) medicine in professional care and conservation
Authors: Yarto-Jaramillo Enrique , Çitaku Irindi , Rodríguez Carlos Enrique , Lewy Sánchez-Aldana Claudia , Morales Mary Carmen , Moresco Anneke
Published: September 2022

Howler monkeys (Alouatta spp.) are threatened by anthropogenic pressures such as habitat fragmentation and deforestation, while conservation efforts are challenging to coordinate as natural geographic distribution ranges are the largest of any New World primate. On a One Health front, howler monkeys represent a great model to investigate the infectious disease dynamics between wild primates and humans as several infectious diseases affecting howlers have a demonstrated zoonotic potential. Howler monkey populations in professional care offer a window to investigate susceptibility to diseases in this species such as yellow fever (YF) and malaria, plus a myriad of endoparasite phyla, as well as vector-borne diseases such as Chagas disease and leishmaniasis. More studies are urgently needed to provide species-specific, medically relevant information as well as clinical descriptions of animals considered medically healthy. Moreover, howler monkeys are a challenging species to breed and maintain in professional care; additionally, reproductive parameters have been published only for a handful of species in this genus. On a One Health approach communication and collaborative health surveillance involving wildlife and zoo experts will ease the identification of factors that contribute to disease emergence facilitating the integration of human, animal, and environmental health. The One Welfare concept is based on the inextricable connection among animal welfare, human welfare, and environmental conservation. Integrating One Health and One Welfare into actions both in-situ and ex-situ will promote the sustainability of the forests and restoration of the ecosystems that those species inhabit, transitioning to a comprehensive One Conservation approach.
Space, time, and context drive anticipatory behavior: Considerations for understanding the behavior of animals in human care
Authors: Krebs Bethany L. , Chudeau Karli R. , Eschmann Caitlin L. , Tu Celina W. , Pacheco Eridia , Watters Jason V.
Published: September 2022

Animal-based measures reflecting the welfare state of individuals are critical for ensuring the well-being of animals under human care. Anticipatory behavior is one potential animal-based measure that has gained traction in recent years, as it is theorized to relate to animals' reward sensitivity. It is of particular interest as an assessment for animals living under human care, as the predictability of the captive environment lends itself to the development of this class of behaviors. Animals are likely to exhibit anticipation in locations related to the anticipated event, often in temporally predictable time frames, and before specific contexts they experience in their day-to-day management. In this sense and under certain circumstances, anticipatory behaviors are likely to drive observed behavioral or space use patterns of animals under human care. Drawing conclusions from such data without identifying anticipation may result in misleading conclusions. Here we discuss how space, time, and context are related to patterns of anticipatory behaviors in animals under human care, how unidentified anticipation may alter conclusions regarding animal behavior or welfare under certain circumstances.
Comparison of Cancer Survival Trends in Hungary in the Periods 2001–2005 and 2011–2015 According to a Population-Based Cancer Registry
Authors: Kenessey István , Szőke Georgina , Dobozi Mária , Szatmári István , Wéber András , Fogarassy György , Nagy Péter , Kásler Miklós , Polgár Csaba , Vathy-Fogarassy Ágnes
Published: September 2022

Background: Assessment of population-based cancer survival may provide the most valuable feedback about the effectiveness of oncological surveillance and treatment.

Aims: Based on the database of the Hungarian National Cancer Registry, standardized incidence rates of lung, breast, colorectal, prostate and cervical cancer were compared to standardized mortality data of the Hungarian Central Statistical Office in the period between 2001 and 2015. Then survival analysis was performed on cleansed database.

Results: The incidence of colorectal, breast and prostate cancer increased, while standardized rates of lung and cervical cancer declined. The survival of colorectal, breast and prostate cancer showed improvement. Contrarily, lung cancer exhibited a mild decline, while that of cervical cancer did not change significantly. In earlier stages survival was improved among almost every studied tumor type, while in advanced stages improvement was not observed. Comparison of stage distribution revealed that in the 2011–2015 period colorectal, breast and prostate cancer cases were diagnosed at earlier stages, while lung and cervical cancer patients were typically discovered at more advanced stages.

Discussion: The outcome of advanced cancer treatments is better in earlier stages, which highlighted the importance of screening network. However, growth of oncological treatment costs with longer patient survival imposes a constantly increasing burden on society.
Identification and Analysis of MADS-box, WRKY, NAC, and SBP-box Transcription Factor Families in Diospyros oleifera Cheng and Their Associations with Sex Differentiation
Authors: Mai Yini , Diao Songfeng , Yuan Jiaying , Wang Liyuan , Suo Yujing , Li Huawei , Han Weijuan , Wang Yiru , Ye Lingshuai , Liu Yang , Pu Tingting , Zhang Qi , Sun Peng , Fu Jianmin
Published: September 2022

Transcription factors are crucial for plant growth and development. MADS-box, WRKY, NAC, and SBP-box, some of the most vital transcription factors, are not well identified in Diospyros spp., and their floral development and sex differentiation related functions remain unknown. We identified and analysed 53 MADS-box, 66 WRKY, 83 NAC, and 17 SBP-box transcription factors using the chromosomal D. oleifera genome. There were six DolSBPs identified as miR156 and miR157 targets. According to the multiple sequence alignments of Arabidopsis and D. oleifera proteins and their conserved domains and motifs, DolMADSs were divided into 23 type I and 30 type II. The DolWRKYs, DolNACs, and DolSBPs were divided into 7, 16, and 6 subgroups, respectively. It was found that one DolMADS, five DolWRKYs, one DolNAC, and four DolSBPs may promote unisexual female flowers development, while three DolMADSs, four DolWRKYs, and one DolNAC may enhance unisexual male flowers development. The functions among the different members of the same family may, thus, vary widely. The results will help to elucidate the evolution of the MADS-box, WRKY, NAC, and SBP-box genes families in D. oleifera and to determine their functions in flower development for Diospyros spp.
Steering Away from Current Amoxicillin Dose Reductions in Hospitalized Patients with Impaired Kidney Function to Avoid Subtherapeutic Drug Exposure
Authors: Smit Cornelis , Sen Swapnoleena , von Dach Elodie , Karmime Abderrahim , Lescuyer Pierre , Tonoli David , Bielicki Julia , Huttner Angela , Pfister Marc
Published: September 2022

Current dose reductions recommended for amoxicillin in patients with impaired kidney function could lead to suboptimal treatments. In a prospective, observational study in hospitalized adults with varying kidney function treated with an IV or oral dose of amoxicillin, amoxicillin concentrations were measured in 1–2 samples on the second day of treatment. Pharmacometric modelling and simulations were performed to evaluate the probability of target attainment (PTA) for 40% of the time above MIC following standard (1000 mg q6h), reduced or increased IV dosing strategies. A total of 210 amoxicillin samples was collected from 155 patients with kidney function based on a CKD-EPI of between 12 and 165 mL/min/1.73 m2. Amoxicillin clearance could be well predicted with body weight and CKD-EPI. Recommended dose adjustments resulted in a clinically relevant reduction in the PTA for the nonspecies-related PK/PD breakpoint MIC of 8 mg/L (92%, 62% and 38% with a CKD-EPI of 10, 20 and 30 mL/min/1.73 m2, respectively, versus 100% for the standard dose). For MICs ≤ 2 mg/L, PTA > 90% was reached in these patients following both reduced and standard dose regimens. Our study showed that for amoxicillin, recommended dose reductions with impaired kidney function could lead to subtherapeutic amoxicillin concentrations in hospitalized patients, especially when targeting less susceptible pathogens.
Do Different Sutures with Triclosan Have Different Antimicrobial Activities? A Pharmacodynamic Approach
Authors: Daoud Frederic C. , M’Zali Fatima , Zabala Arnaud , Moore Nicholas , Rogues Anne-Marie
Published: September 2022

(1) Background: Three antimicrobial absorbable sutures have different triclosan (TS) loads, triclosan release kinetics and hydrolysis times. This in vitro study aims to analyse and compare their antimicrobial pharmacodynamics. (2) Methods: Time-kill assays were performed with eight triclosan-susceptible microorganisms common in surgical site infections (SSIs) and a segment of each TS. Microbial concentrations were measured at T0, T4, T8 and T24 h. Similar non-triclosan sutures (NTS) were used as controls. Microbial concentrations were plotted and analysed with panel analysis. They were predicted over time with a double-exponential model and four parameters fitted to each TS × microorganism combination. (3) Results: The microbial concentration was associated with the triclosan presence, timeslot and microorganism. It was not associated with the suture material. All combinations shared a common pattern with an early steep concentration reduction from baseline to 4–8 h, followed by a concentration up to a 24-h plateau in most cases with a mild concentration increase. (4) Conclusions: Microorganisms seem to be predominantly killed by contact or near-contact killing with the suture rather than the triclosan concentration in the culture medium. No significant in vitro antimicrobial pharmacodynamic difference between the three TS is identified. Triclosan can reduce the suture microbial colonisation and SSI risk.
In Vitro and In Vivo Antimicrobial Activity of the Novel Peptide OMN6 against Multidrug-Resistant Acinetobacter baumannii
Authors: Michaeli Janna , Mandel Shira , Maximov Shelly , Zazoun Jonathan , Savoia Paola , Kothari Nimmi , Valmont Thomas , Ferrari Livia , Duncan Leonard R. , Hawser Stephen , Cohen-Kutner Moshe , Bachnoff Niv
Published: September 2022

The rapid worldwide spread of antimicrobial resistance highlights the significant need for the development of innovative treatments to fight multidrug-resistant bacteria. This study describes the potent antimicrobial activity of the novel peptide OMN6 against a wide array of drug-resistant Acinetobacter baumannii clinical isolates. OMN6 prevented the growth of all tested isolates, regardless of any pre-existing resistance mechanisms. Moreover, in vitro serial-passaging studies demonstrated that no resistance developed against OMN6. Importantly, OMN6 was highly efficacious in treating animal models of lung and blood infections caused by multidrug-resistant A. baumannii. Taken together, these results point to OMN6 as a novel antimicrobial agent with the potential to treat life-threatening infections caused by multidrug-resistant A. baumannii avoiding resistance.
Anaerobic Digestion of Pig-Manure Solids at Low Temperatures: Start-Up Strategies and Effects of Mode of Operation, Adapted Inoculum, and Bedding Material
Authors: Rajagopal Rajinikanth , Bele Vaibhavi , Saady Noori M. Cata , Hickmann Felipe M. W. , Goyette Bernard
Published: September 2022

The objective of this study was to obtain start-up strategies for the operation of a dry anaerobic digestion (DAD) system treating pig-manure (PM) solids at low-temperatures, and evaluate the effects of operation mode, adapted inoculum, and bedding material on the performance. A DAD system coupled with an inoculum system (two-stage DAD) was operated at 20 ± 1 °C to digest PM solids (Total Solids, TS: 27%) with wheat straw or woodchips as bedding materials (TS substrate-mixture: 45%) using a liquid inoculum. Static DAD was also operated in parallel for comparison purposes. Overall, the percolation–recirculation mode of operation was superior to the static mode; the former had more than a 3-fold increase in specific methane yield in cycle 3. Using the adapted inoculum in cycle-2 improved methane yield by 7% and 26% for cycles 1 and 3, respectively, under the percolation–recirculation mode of operation. In addition, the digestate resulting from the digestion of woodchips + PM solids had better physical characteristics than wheat straw + PM solids. Thus, anaerobic digestion of pig-manure solids at low-temperatures with appropriate start-up strategies, inoculum, and bedding material is a promising technology for transforming PM solids into biogas and using its digestate as biofertilizer.
A Multimodality Myocardial Perfusion Phantom: Initial Quantitative Imaging Results
Authors: Kamphuis Marije E. , Kuipers Henny , Liefers H. Remco , van Es Jan , Simonis Frank F. J. , Greuter Marcel J. W. , Slump Cornelis H. , Slart Riemer H. J. A.
Published: September 2022

This proof-of-concept study explores the multimodal application of a dedicated cardiac flow phantom for ground truth contrast measurements in dynamic myocardial perfusion imaging with CT, PET/CT, and MRI. A 3D-printed cardiac flow phantom and flow circuit mimics the shape of the left ventricular cavity (LVC) and three myocardial regions. The regions are filled with tissue-mimicking materials and the flow circuit regulates and measures contrast flow through LVC and myocardial regions. Normal tissue perfusion and perfusion deficits were simulated. Phantom measurements in PET/CT, CT, and MRI were evaluated with clinically used hardware and software. The reference arterial input flow was 4.0 L/min and myocardial flow 80 mL/min, corresponding to myocardial blood flow (MBF) of 1.6 mL/g/min. The phantom demonstrated successful completion of all processes involved in quantitative, multimodal myocardial perfusion imaging (MPI) applications. Contrast kinetics in time intensity curves were in line with expectations for a mimicked perfusion deficit (38 s vs. 32 s in normal tissue). Derived MBF in PET/CT and CT led to under- and overestimation of reference flow of 0.9 mL/g/min and 4.5 mL/g/min, respectively. Simulated perfusion deficit (0.8 mL/g/min) in CT resulted in MBF of 2.8 mL/g/min. We successfully performed initial, quantitative perfusion measurements with a dedicated phantom setup utilizing clinical hardware and software. These results showcase the multimodal phantom’s potential.
Green Production and Interaction of Carboxylated CNTs/Biogenic ZnO Composite for Antibacterial Activity
Authors: Hussain Saghir , Khakwani Noorulain , Faiz Yasir , Zulfiqar Sonia , Shafiq Zahid , Faiz Faisal , Elhakem Abeer , Sami Rokayya , Aljuraide N. I. , Farid Tanveer , Aljabri Mahmood D. , Rahman Mohammed M.
Published: September 2022

Using biomolecule-rich plant extracts, the conversion of metal ions to metal oxide nanoparticles via abiogenic approach is highly intriguing, environmentally friendly, and quick. The inherent inclination of plant extracts function as capping agents in the insitu synthesis. In this study, biogenic zinc oxide nanoparticles (ZnO−NPs) were synthesized using an aqueous leaf extract from Moringaoleifera. The ZnO−NPs were then mixed with carboxylated carbon nanotubes (CNTs) to create a carboxylated CNTs/biogenic ZnO composite using asol–gel method. The CNTs/ZnO composite displayed 18 mm, 16 mm, and 17 mm zones of inhibition (ZOI) against Bacillus cereus, Pseudomonas aeruginosa, and Escherichia coli, respectively. In contrast with ZnO−NPs, the produced carboxylated CNTs/ZnO composite demonstrated a 13 percent elevation in ZOI as antibacterial activity against Bacillus cereus ATCC 19659, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853. The characterization of ZnO−NPs and the carboxylated CNTs/ZnO composite were performed via FTIR, UV/Vis spectroscopy, SEM, and XRD. The XRD pattern depicted a nano−sized crystalline structure (Wurtzite) of ZnO−NPs and a carboxylated CNTs/ZnO composite. The current work comprehends a valuable green technique for killing pathogenic bacteria, and gives fresh insights into the manufacture of metal oxide composites for future research.
Differential Sensitization of Muscle versus Fascia in Individuals with Low Back Pain
Authors: Cozacov Ronen , Minerbi Amir , Haddad May , Vulfsons Simon
Published: September 2022

Muscles and the deep fascia surrounding them have been suggested to play an important role in various musculoskeletal pain conditions including low back pain. Both have been shown to host rich nociceptive innervation and to undergo changes in individuals with chronic pain. However, evidence for the respective contribution of muscle and fascia sensitization in humans with myofascial pain syndrome is lacking. Here, we studied the sensitization of muscle and fascia in individuals with myofascial low back pain. Twenty individuals with acute (5) and chronic (15) myofascial low back pain of the quadratus lumborum muscle and a matched control group of twenty healthy individuals were recruited and clinically evaluated. All participants underwent ultrasound-guided needling of their subcutaneous tissue, deep fascia and quadratus lumborum muscle. Reported pain intensity and episodes of muscle twitching were recorded and analyzed. Among pain patients, both muscles and deep fascia demonstrated pain hypersensitivity, but muscles were significantly more sensitized than the deep fascia. No difference between acute- or chronic-pain patients was observed. Results of this study suggest that while both deep fascia and muscle show pain sensitization in both early and chronic stages of low back pain, muscles are more sensitized than fascia.