bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of an Exercise Program Combining Aerobic and Resistance Training on Protein Expressions of Neurotrophic Factors in Obese Rats Injected with Beta-Amyloid
Authors: Lee Gyuho , Kim Yunwook , Jang Jung-Hee , Lee Chan , Yoon Jaewoo , Ahn Nayoung , Kim Kijin
Published: June 2022

In this study, the effects of a 12-week exercise program combining aerobic and resistance training on high-fat diet-induced obese Sprague Dawley (SD) rats after the injection of beta-amyloid into the cerebral ventricle were investigated. Changes in physical fitness, cognitive function, blood levels of beta-amyloid and metabolic factors, and protein expressions of neurotrophic factors related to brain function such as BDNF (brain-derived neurotrophic factor) in the quadriceps femoris, hippocampus, and cerebral cortex were analyzed. The subjects were thirty-two 10-week-old SD rats (DBL Co., Ltd., Seoul, Korea). The rats were randomized into four groups: β-Non-Ex group (n = 8) with induced obesity and βA25-35 injection into the cerebral ventricle through stereotactic biopsy; β-Ex group (n = 8) with induced obesity, βA25-35 injection, and exercise; S-Non-Ex group (n = 8) with an injection of saline in lieu of βA25-35 as the control; and S-Ex group (n = 8) with saline injection and exercise. The 12-week exercise program combined aerobic training and resistance training. As for protein expressions of the factors related to brain function, the combined exercise program was shown to have a clear effect on activating the following factors: PGC-1α (peroxisome proliferator-activated receptor gamma coactivator 1-alpha), FNDC5 (fibronectin type III domain-containing protein 5), and BDNF in the quadriceps femoris; TrkB (Tropomyosin receptor kinase B), FNDC5, and BDNF in the hippocampus; PGC-1α, FNDC5, and BDNF in the cerebral cortex. The protein expression of β-amyloid in the cerebral cortex was significantly lower in the β-Ex group than in the β-Non-Ex group (p < 0.05). The 12-week intervention with the combined exercise program of aerobic and resistance training was shown to improve cardiopulmonary function, muscular endurance, and short-term memory. The results demonstrate a set of positive effects of the combined exercise program, which were presumed to have arisen mainly due to its alleviating effect on β-amyloid plaques, the main cause of reduced brain function, as well as the promotion of protein expressions of PGC-1α, FNDC5, and BDNF in the quadriceps femoris, hippocampus, and cerebral cortex.
Knowledge, Attitude, and Perception of Cancer Patients towards COVID-19 in Pakistan: A Cross-Sectional Study
Authors: Khattak Saadullah , Faheem Muhammad , Nawaz Bilawal , Khan Maqbool , Khan Nazeer Hussain , Ullah Nadeem , Khan Taj Ali , Khan Rahat Ullah , Haleem Kashif Syed , Ren Zhi-Guang , Wu Dong-Dong , Ji Xin-Ying
Published: June 2022

Background: Cancer patients, being immunocompromised, are at higher risk of coronavirus disease (COVID-19). The current study determines cancer patients’ knowledge, attitude, perception, and impact of the COVID-19 pandemic. Method: A cross-sectional online survey was conducted in Pakistan from 1 April 2020 to 1 May 2020. The study respondents were cancer patients with ages equal to or greater than 18 years. Following a request for participation, the URL for the survey was distributed on numerous channels. Other social media platforms, including WeChat, WhatsApp, Facebook, Twitter, Instagram, Messenger, and LinkedIn, were used to increase cancer patient interaction. The questionnaire comprised five different sections such as: (1) sociodemographic information, (2) knowledge, (3) attitude, (4) perception, and (5) impact of COVID-19 on cancer patients. Descriptive medical statistics such as frequency, percentage, mean, and standard deviation were used to illustrate the demographic characteristics of the study participants. To compare mean knowledge scores with selected demographic variables, independent sample t-tests and one-way analysis of variance (ANOVA) were used, which are also practical methods in epidemiological, public health and medical research. The cut-off point for statistical significance was set at a p-value of 0.05. Results: More than 300 cancer patients were invited, of which 208 agreed to take part. The response rate was 69.33% (208/300). Gender, marital status, and employment status had a significant association with knowledge scores. Of the total recruited participants, 96% (n = 200) (p < 0.01) knew about COVID-19, and 90% were aware of general symptoms of COVID-19 disease, such as route of transmission and preventive measurements. In total, 94.5% (n = 197) (p < 0.01) were willing to accept isolation if they were infected with COVID-19, and 98% (n = 204) (p < 0.01) had reduced their use of public transportation. More than 90% (n = 188) (p < 0.01) of cancer patients were found to be practicing preventative measures such as using a face mask, keeping social distance, and avoiding handshaking and hugging. Around 94.4% (n = 196) (p < 0.01) of cancer patients had been impacted by, stopped or had changed cancer treatment during this pandemic, resulting in COVID-related anxiety and depression. Conclusion: The included cancer patients exhibited a good level of COVID-19 knowledge, awareness, positive attitude, and perception. Large-scale studies and efforts are needed to raise COVID-19 awareness among less educated and high-risk populations. The present survey indicates that mass-level effective health education initiatives are required for developing countries to improve and reduce the gap between KAP and COVID-19.
Study on Spatial Distribution Equilibrium of Elderly Care Facilities in Downtown Shanghai
Authors: Huang Xiaoran , Gong Pixin , White Marcus
Published: June 2022

With the growing challenge of aging populations around the world, the study of the care services for older adults is an essential initiative to accommodate the particular needs of the disadvantaged communities and promote social equity. Based on open-source data and the geographic information system (GIS), this paper quantifies and visualizes the imbalance in the spatial distribution of elderly care facilities in 14,578 neighborhoods in downtown (seven districts) Shanghai, China. Eight types of elderly care facilities were obtained from Shanghai elderly care service platform, divided into two categories according to their service scale. With the introduction of the improved Gaussian 2-step floating catchment area method, the accessibility of two category facilities was calculated. Through the global autocorrelation analysis, it is found that the accessibility of elderly care facilities has the characteristics of spatial agglomeration. Local autocorrelation analysis indicates the cold and hot spots in the accessibility agglomeration state of the two types of facilities, by which we summarized the characteristics of their spatial heterogeneity. It is found that for Category−I, there is a large range of hot spots in Huangpu District. For Category−II, the hot-spot and cold-spot areas show staggered distribution, and the two categories of hot spot distribution show a negative correlation. We conclude that the two categories are not evenly distributed in the urban area, which will lead to the low efficiency of resource allocation of elderly care facilities and have a negative impact on social fairness. This research offers a systematic method to study urban access to care services for older adults as well as a new perspective on improving social fairness.
Effects of Health Status, Depression, Gerotranscendence, Self-Efficacy, and Social Support on Healthy Aging in the Older Adults with Chronic Diseases
Authors: Kim Hee-Kyung , Seo Jeong-Hyo
Published: June 2022

Purpose of this study is to analyze factors affecting the healthy aging of the elderly with chronic diseases living in the community according to the worldwide aging phenomenon in line with the WHO’s healthy aging strategy. The subjects were 116 elderly aged 65 years or up with one or more chronic diseases and residing in four cities. The collected data were analyzed by using the descriptive statistics, t-test, ANOVA, Pearson’s correlational coefficients, and stepwise multiple regression. The healthy aging of subjects showed positive correlations with the health status (r = 0.68, p < 0.001), gerotranscendence (r = 0.64, p < 0.001), self-efficacy (r = 0.65, p < 0.001), and social support (r = 0.47, p < 0.001), while the healthy aging and depression (r = −0.58, p < 0.001) showed a negative correlation. The factors affecting the healthy aging were health status (β = 0.24, p = 0.004), self-efficacy (β = 0.28, p < 0.001), education (β = −0.11, p = 0.057), exercise (β = 0.17, p = 0.003), gerotranscendence (β = 0.22, p = 0.004), and depression (β = −0.19, p = 0.009), and the explanatory power of those variables was 68.2%. Thus, it would be necessary to provide an intervention for the elderly that could habituate health-related education and exercise, maintain good health status, lower depression, aid control of themselves through the gerotranscendence, and increase self-efficacy.
“We Don’t Have to Do Things the Way They’ve Been Done Before”; Mixed-Method Evaluation of a National Grant Program Tackling Physical Inactivity through Sport
Authors: Rose Catriona L. , Owen Katherine B. , Foley Bridget C. , Reece Lindsey J.
Published: June 2022

National strategies are needed to continue to promote the broader benefits of participating in sport and organised physical activity to reduce physical inactivity and related disease burden. This paper employs the RE-AIM framework to evaluate the impact of the federally funded $150 million Move it AUS program in engaging inactive people in sport and physical activity through the Participation (all ages) and Better Ageing (over 65 years) funding streams. A pragmatic, mixed-methods evaluation was conducted to understand the impact of the grant on both the participants, and the funded organisations. This included participant surveys, case studies, and qualitative interviews with funded program leaders. A total of 75% of participants in the Participation stream, and 65% in the Better Ageing stream, were classified as inactive. The largest changes in overall physical activity behaviour were seen among socioeconomically disadvantaged participants and culturally and linguistically diverse participants. Seven key insights were gained from the qualitative interviews: Clarity of who, Partnerships, Communication, Program delivery, Environmental impacts, Governance, and that Physical inactivity must be a priority. The Move It AUS program successfully engaged physically inactive participants. Additional work is needed to better engage inactive people that identify as culturally and linguistically diverse, Aboriginal and/or Torres Strait Islander and those that live in disadvantaged communities in sport and physical activities. Tangible actions from the seven key insights should be adopted into workforce capability planning for the sport sector to effectively engage physically inactive communities.
Financial Expenses and “Losses” of the Polish Healthcare System Resulting from the Occurrence of Adverse Events
Authors: Leśniak Tomasz , Sierocka Aleksandra , Kostrzewa Dariusz , Kozłowski Remigiusz , Marczak Michał
Published: June 2022

Background: The globally increasing healthcare expenditures related to the need to treat the consequences of adverse events, as well as the number of claims filed by patients (or their families) and remuneration paid as their result mean that the interest in the subject of adverse event cost management is increasing. An increase in the number of cases concerning medical errors has also occurred in Poland in recent years. The newest statistics from the Ministry of Justice demonstrate that the courts are awarding increasingly higher amounts. The goal of this work was an attempt to approximate, based on our own experiences, the impact of adverse events on the expenditures of the healthcare system in Poland, including the costs of treatment of the consequences of such events, described by the authors as “secondary harm”. Methods: Based on the analysis of 100 cases for compensation for the occurrence of a medical event, an initial estimate of the costs of primary (initial) treatment, which resulted in the occurrence of the adverse event, and the costs of subsequent hospitalisations/stays, which were its consequences. The study was conducted in the period from October 2020 to November of 2021. Results: The statistical analysis of the examined cases enabled establishing that in 62% they concerned women. Only 38% were events which applied to men. The highest number of cases concerned events which occurred in the last years, that is 2018 (35%), 2019 (23%), and 2017 (17%). The most frequent events included those related to incorrect diagnosis (the lack of correct diagnosis), which resulted in appropriate activities not being undertaken and a lack of appropriate treatment, e.g., lack of diagnosis of cancer, myocardial infarction, appendicitis, or fracture (26%). The next one was incorrect surgical treatment (17%)—the consequence of which was most frequently a need for repeated surgery and an incorrect conservative treatment of injuries. The obtained results demonstrate that significantly higher funds are spent by medical entities for “restorative” actions (on average EUR 1433, which attempt to mitigate against the negative consequences of incorrect decisions or actions in the original treatment (average cost of EUR 814)). Conclusions: The consequences of adverse events include not only health-related harm for the patient, but also long-term social, familial, or professional results. The authors of the article are of an opinion that all the conducted analyses and conclusions drawn from them should serve the improvement of patient safety. They also form an initial point for establishing recommendations and advice for the improvement of safety and quality of medical services and the reduction of healthcare-related costs. The authors propose covering the parties injured by an adverse event (subjected to “secondary harm”) with a unique, innovative programme of post-accident health care, “Health Reconstruction”.
Validity of Diagnostic Algorithms for Inflammatory Bowel Disease in Japanese Hospital Claims Data
Authors: Takahashi Sayumi , Obara Taku , Kakuta Yoichi , Shimoyama Yusuke , Naito Takeo , Moroi Rintaro , Kuroha Masatake , Shiga Hisashi , Kinouchi Yoshitaka , Masamune Atsushi
Published: June 2022

Inflammatory bowel disease (IBD) diagnoses are increasing in Japan. Some patients have symptoms that are difficult to control, and further research on IBD is needed. Claims databases, which have a large sample size, can be useful for IBD research. However, it is unclear whether the International Classification of Diseases, Tenth Revision (ICD-10) codes alone can correctly identify IBD. We aimed to develop algorithms to identify IBD in claims databases. We used claims data from the Department of Gastroenterology, Tohoku University Hospital from 1 January 2016 to 31 December 2020. We developed 11 algorithms by combining the ICD-10 code, prescription drug, and workup information. We had access to the database which contains all the information for Crohn’s disease and ulcerative colitis patients who visited our department, and we used it as the gold standard. We calculated sensitivity, specificity, positive predictive value (PPV), and negative predictive value for each algorithm. We enrolled 19,384 patients, and among them, 1012 IBD patients were identified in the gold standard database. Among 11 algorithms, Algorithm 4 (ICD-10 code and ≥1 prescription drugs) showed a strong performance (PPV, 94.8%; sensitivity, 75.6%). The combination of an ICD-10 code and prescription drugs may be useful for identifying IBD among claims data.
Histological and Biological Response to Different Types of Biomaterials: A Narrative Single Research Center Experience over Three Decades
Authors: Tumedei Margherita , Mijiritsky Eitan , Mourão Carlos Fernando , Piattelli Adriano , Degidi Marco , Mangano Carlo , Iezzi Giovanna
Published: June 2022

Background: In more than three decades of work of the Retrieval Bank of the Laboratory for Undemineralized Hard Tissue Histology of the University of Chieti-Pescara in Italy, many types of biomaterials were received and evaluated. The present retrospective review aimed to evaluate the histological and biological aspects of the evaluated bone substitute biomaterials. Methods: In the present study, the authors prepared a retrospective analysis after the screening of some databases (PubMed, Scopus, and EMBASE) to find papers published from the Retrieval Bank of the Laboratory for Undermineralized Hard Tissue Histology of the University of Chieti-Pescara analyzing only the papers dealing with bone substitute biomaterials and scaffolds, in the form of granules and block grafts, for bone regeneration procedures. Results: Fifty-two articles were found, including in vitro, in vivo, and clinical studies of different biomaterials. These articles were evaluated and organized in tables for a better understanding. Conclusions: Over three decades of studies have made it possible to assess the quality of many bone substitute biomaterials, helping to improve the physicochemical and biological properties of the biomaterials used in daily clinical practice.
How Does Clean Energy Consumption Affect Women’s Health: New Insights from China
Authors: Li Fanghua , Chandio Abbas Ali , Duan Yinying , Zang Dungang
Published: June 2022

The United Nations (UN) has identified the promotion of cleaner energy and improving women’s health as two important elements in achieving the global sustainable development goals. However, the impact of household clean energy consumption on women’s health needs to be further analyzed and improved based on new methods, new data, and new perspectives. This paper used the data from the 2018 China Health and Retirement Longitudinal Study as the sample, and the Ordered Probit model, the instrumental variable (IV) approach, the conditional mixed process (CMP) method, and the mechanism analysis model were applied to empirically investigate the impact of cleaner household energy consumption on women’s health. The findings are the following: (1) It is found that cleaner household energy consumption improved women’s health, and after selecting “respondent’s regions of residence” as an IV to overcome endogenous issues, the estimated results remained significant. (2) The mechanistic estimation showed that air quality, social contact, and well-being play a mediating role in the effects of cleaner household energy consumption on women’s health, while digital ability plays a moderating role in the cleaner household energy consumption impact on women’s health. (3) This study further explored that cleaner household energy consumption significantly reduced the likelihood of women being diagnosed with hypertension, hyperlipidemia, cancer, lung disease, asthma, and depression. The conclusion of this paper that “cleaner household energy can enhance the level of women’s health” supports the viewpoints of some present literature. At the same time, this paper puts forward four policy recommendations based on the research conclusions.
Higher Levels of Postnatal Depressive Symptomatology, Post-Traumatic Growth, and Life Satisfaction among Gay Fathers through Surrogacy in Comparison to Heterosexual Fathers: A Study in Israel in Times of COVID-19
Authors: Shenkman Geva , Levy Sigal , Winkler Ziv Ben-Dor , Bass Doriel , Geller Shulamit
Published: June 2022

This study aimed to explore the psychological welfare, as indicated by postnatal depressive symptomatology, life satisfaction, and posttraumatic growth (growth after contending with stressful birth events), of Israeli gay fathers through surrogacy in comparison to heterosexual fathers. For that purpose, a sample of 167 Israeli fathers (M = 35.6, SD = 4.4) was recruited (68 identified as gay fathers through surrogacy and 99 as heterosexual fathers). Participants completed questionnaires assessing their postnatal depressive symptomatology, life satisfaction, and sense of posttraumatic growth after becoming fathers. Results indicated that gay fathers through surrogacy reported higher levels of life satisfaction and posttraumatic growth than heterosexual fathers. Yet, gay fathers also reported higher levels of postnatal depressive symptomatology than heterosexual fathers when life satisfaction or posttraumatic growth values were low or medium. The findings were interpreted in light of the hardships associated with cross-border surrogacy and the psychological outcomes associated with succeeding to become fathers after contending with them. The study contributes to the limited literature on postnatal depressive symptomatology and posttraumatic growth among gay fathers through surrogacy and provides clinicians and policymakers with relevant information on the psychological strengths and potential difficulties associated with cross-border surrogacy among gay fathers.
Development of Public Health Core Outcome Sets for Systems-Wide Promotion of Early Life Health and Wellbeing
Authors: Mansukoski Liina , Albert Alexandra , Vafai Yassaman , Cartwright Chris , Rahman Aamnah , Sheringham Jessica , Lockyer Bridget , Yang Tiffany C. , Garnett Philip , Bryant Maria
Published: June 2022

We aimed to develop a core outcome set (COS) for systems-wide public health interventions seeking to promote early life health and wellbeing. Research was embedded within the existing systems-based intervention research programme ‘ActEarly’, located in two different areas with high rates of child poverty, Bradford (West Yorkshire) and the Borough of Tower Hamlets (London). 168 potential outcomes were derived from five local government outcome frameworks, a community-led survey and an ActEarly consortium workshop. Two rounds of a Delphi study (Round 1: 37 participants; Round 2: 56 participants) reduced the number of outcomes to 64. 199 members of the community then took part in consultations across ActEarly sites, resulting in a final COS for systems-based public health interventions of 40 outcomes. These were grouped into the domains of: Development & education (N = 6); Physical health & health behaviors (N = 6); Mental health (N = 5); Social environment (N = 4); Physical environment (N = 7); and Poverty & inequality (N = 7). This process has led to a COS with outcomes prioritized from the perspectives of local communities. It provides the means to increase standardization and guide the selection of outcome measures for systems-based evaluation of public health programmes and supports evaluation of individual interventions within system change approaches.
New 2-[(4-Amino-6-N-substituted-1,3,5-triazin-2-yl)methylthio]-N-(imidazolidin-2-ylidene)-4-chloro-5-methylbenzenesulfonamide Derivatives, Design, Synthesis and Anticancer Evaluation
Authors: Tomorowicz Łukasz , Żołnowska Beata , Szafrański Krzysztof , Chojnacki Jarosław , Konopiński Ryszard , Grzybowska Ewa A. , Sławiński Jarosław , Kawiak Anna
Published: June 2022

In the search for new compounds with antitumor activity, new potential anticancer agents were designed as molecular hybrids containing the structures of a triazine ring and a sulfonamide fragment. Applying the synthesis in solution, a base of new sulfonamide derivatives 20–162 was obtained by the reaction of the corresponding esters 11–19 with appropriate biguanide hydrochlorides. The structures of the compounds were confirmed by spectroscopy (IR, NMR), mass spectrometry (HRMS or MALDI-TOF/TOF), elemental analysis (C,H,N) and X-ray crystallography. The cytotoxic activity of the obtained compounds toward three tumor cell lines, HCT-116, MCF-7 and HeLa, was examined. The results showed that some of the most active compounds belonged to the R1 = 4-trifluoromethylbenzyl and R1 = 3,5-bis(trifluoromethyl)benzyl series and exhibited IC50 values ranging from 3.6 µM to 11.0 µM. The SAR relationships were described, indicating the key role of the R2 = 4-phenylpiperazin-1-yl substituent for the cytotoxic activity against the HCT-116 and MCF-7 lines. The studies regarding the mechanism of action of the active compounds included the assessment of the inhibition of MDM2-p53 interactions, cell cycle analysis and apoptosis induction examination. The results indicated that the studied compounds did not inhibit MDM2-p53 interactions but induced G0/G1 and G2/M cell cycle arrest in a p53-independent manner. Furthermore, the active compounds induced apoptosis in cells harboring wild-type and mutant p53. The compound design was conducted step by step and assisted by QSAR models that correlated the activity of the compounds against the HCT-116 cell line with molecular descriptors.
Neuroimmune Mechanisms Underlying Neuropathic Pain: The Potential Role of TNF-α-Necroptosis Pathway
Authors: Duan Yi-Wen , Chen Shao-Xia , Li Qiao-Yun , Zang Ying
Published: June 2022

The neuroimmune mechanism underlying neuropathic pain has been extensively studied. Tumor necrosis factor-alpha (TNF-α), a key pro-inflammatory cytokine that drives cytokine storm and stimulates a cascade of other cytokines in pain-related pathways, induces and modulates neuropathic pain by facilitating peripheral (primary afferents) and central (spinal cord) sensitization. Functionally, TNF-α controls the balance between cell survival and death by inducing an inflammatory response and two programmed cell death mechanisms (apoptosis and necroptosis). Necroptosis, a novel form of programmed cell death, is receiving increasing attraction and may trigger neuroinflammation to promote neuropathic pain. Chronic pain is often accompanied by adverse pain-associated emotional reactions and cognitive disorders. Overproduction of TNF-α in supraspinal structures such as the anterior cingulate cortex (ACC) and hippocampus plays an important role in pain-associated emotional disorders and memory deficits and also participates in the modulation of pain transduction. At present, studies reporting on the role of the TNF-α–necroptosis pathway in pain-related disorders are lacking. This review indicates the important research prospects of this pathway in pain modulation based on its role in anxiety, depression and memory deficits associated with other neurodegenerative diseases. In addition, we have summarized studies related to the underlying mechanisms of neuropathic pain mediated by TNF-α and discussed the role of the TNF-α–necroptosis pathway in detail, which may represent an avenue for future therapeutic intervention.
Glioblastoma Extracellular Vesicle-Specific Peptides Inhibit EV-Induced Neuronal Cytotoxicity
Authors: Zhou Wenbo , Craft Julia , Ojemann Alex , Bergen Luke , Graner Arin , Gonzales Aitana , He Qianbin , Kopper Timothy , Smith Marie , Graner Michael W. , Yu Xiaoli
Published: June 2022

WHO Grade 4 IDH-wild type astrocytoma (GBM) is the deadliest brain tumor with a poor prognosis. Meningioma (MMA) is a more common “benign” central nervous system tumor but with significant recurrence rates. There is an urgent need for brain tumor biomarkers for early diagnosis and effective treatment options. Extracellular vesicles (EVs) are tiny membrane-enclosed vesicles that play essential functions in cell-to-cell communications among tumor cells. We aimed to identify epitopes of brain tumor EVs by phage peptide libraries. EVs from GBM plasma, MMA plasma, or brain tumor cell lines were used to screen phage-displayed random peptide libraries to identify high-affinity peptides. We purified EVs from three GBM plasma pools (23 patients), one MMA pool (10 patients), and four brain tumor cell lines. We identified a total of 21 high-affinity phage peptides (12 unique) specific to brain tumor EVs. The peptides shared high sequence homologies among those selected by the same EVs. Dose–response ELISA demonstrated that phage peptides were specific to brain tumor EVs compared to controls. Peptide affinity purification identified unique brain tumor EV subpopulations. Significantly, GBM EV peptides inhibit brain tumor EV-induced complement-dependent cytotoxicity (necrosis) in neurons. We conclude that phage display technology could identify specific peptides to isolate and characterize tumor EVs.
Clinical Characteristics and Prognosis of Patients with Multi-Vessel Coronary Spasm in Comparison with Those in Patients with Single-Vessel Coronary Spasm
Authors: Teragawa Hiroki , Oshita Chikage , Uchimura Yuko
Published: June 2022

(1) Background: We have sometimes experienced patients with vasospastic angina (VSA) who presented multi-vessel spasm (MVS) on coronary angiography and spasm provocation test (SPT). However, the clinical characteristics of VSA patients with MVS and the prognosis of such patients in the clinical setting have not been clarified. Therefore, we compared the clinical characteristics and prognosis in VSA patients with MVS with those in VSA patients with single-vessel spasm (SVS). (2) Methods: A total of 152 patients (mean age, 67 years, 74 men and 78 women) with VSA, in which the presence of coronary spasm was assessed in both left coronary artery (LCA) and right coronary artery (RCA) on SPT, were enrolled. We defined VSA as the presence of >90% narrowing of the epicardial coronary artery on angiograms, accompanied by usual chest symptoms and/or ischaemic ST-T changes on the electrocardiogram. On SPT, MVS was defined as the presence of spasms on ≥2 major coronary arteries. Based on the presence of MVS, patients were divided into the MVS group and the SVS group. The frequencies of conventional coronary risk factors, blood chemical parameters, average times of anginal attack, SPT findings such as spasm provocation induced by a low dose of acetylcholine (L-ACh) and total occlusion due to coronary spasm (TOC), number of coronary vasodilators at discharge and major cardiovascular events (MACE, including cardiac death and readmission due to any cause of cardiovascular diseases) were compared between the two groups. (3) Results: The MVS and SVS groups were comprised of 98 (64%) and 54 (36%) patients, respectively. The level of fasting blood glucose (FBS) was lower (p < 0.01), and the level of cystatin-C (n = 89) tended to be higher (p = 0.07) in the MVS group than in the SVS group. The frequencies of L-ACh-induced coronary spasm (33% in MVS and 17% in SVS, p = 0.04) and TOC (12% in MVS, 0% in SVS, p < 0.01) were higher in the MVS group than in the SVS group. The average number of coronary vasodilators at discharge was higher in the MVS group (1.2 ± 0.4) than in the SVS group (0.9 ± 0.5, p < 0.01). The frequency of MACE was not different between the two groups. (4) Conclusions: Patients with MVS may have higher VSA activity on SPT and have more aggressive medications, leading to a comparable prognosis in VSA patients with SVS. MVS is an important indicator of at least VSA activity, and cardiologists should confirm this in SPT whenever possible. Further studies should confirm whether lower FBS levels and higher cystatin-C levels are any markers of MVS.
A Wet/Dry Point Treatment Method of FVCOM, Part I: Stability Experiments
Authors: Chen Changsheng , Qi Jianhua , Liu Hedong , Beardsley Robert , Lin Huichan , Cowles Geoffrey
Published: June 2022

A 3-dimensional wet/dry point treatment method was developed for the unstructured-grid Finite-Volume Community Ocean Model (FVCOM). Analytical equations were derived to examine discretized errors that occurred during the flooding/drying process by the wet/dry point treatment for the flooding/drying process. Numerical experiments were carried out for an idealized estuary, including the inter-tidal zone. The model results show that if the ratio of internal to external mode time steps (Isplit) is appropriately selected, FVCOM was capable of simulating the flooding/drying process with sufficient accuracy to ensure the mass conservation. The up-bound limit of Isplit was restricted by the bathymetric slope of the inter-tidal zone, external mode time step, horizontal/vertical resolution, and amplitude of tidal forcing at the open boundary, as well as the thickness of the viscous layer specified in the model. Criteria for time steps via these parameters were derived from these experiments, which provide a helpful guide in selecting Isplit for applying FVCOM to realistic geometric estuaries.
Improvement of Regasification Process Efficiency for Floating Storage Regasification Unit
Authors: Semaskaite Vigaile , Bogdevicius Marijonas , Paulauskiene Tatjana , Uebe Jochen , Filina-Dawidowicz Ludmila
Published: June 2022

Natural gas plays a vital role in the economically and environmentally sustainable future of energy. Its reliable deliveries are required, especially nowadays, when the energy market is so volatile and unstable. The conversion of natural gas to its liquefied form (LNG) allows its transport in greater quantities. Affordability and reliability of clean energy is a key issue even for developed markets. Therefore, natural gas usage enables to implement green solutions into countries’ economies. However, the LNG-production process consumes a considerable amount of energy. This energy is stored in LNG as cold energy. After LNG unloading into storage tanks at receiving terminals, it is vaporised and compressed for transmission to a natural gas pipeline system. During the regasification process, the large part of the energy stored in LNG may be recovered and used for electricity generation, seawater desalination, cryogenic air separation, hydrogen liquefaction, material freezing, carbon dioxide capture, as well as for combined LNG cold energy utilization systems. Moreover, increased efficiency of LNG terminals may attract potential clients. In the presented paper, a mathematical model is performed to determine the influence of LNG composition and regasification process parameters on the quantity of released LNG cold energy in a large-scale floating storage and regasification units (FSRU)-type terminal “Independence” (Lithuania). Flow rate of LNG regasification, pressure, and boil-off gas recondensation have been considered. Possibilities to reduce the energy losses were investigated to find the ways to improve the regasification process efficiency for real FSRU. The results analysis revealed that potential of LNG cold energy at FSRU could vary from 20 to 25 MW. A utilisation of industrial and urban waste heat for the heat sink FSRU is recommended to increase the energy efficiency of the whole regasification process.
Environmental Compatibility of the Parc Tramuntana Offshore Wind Project in Relation to Marine Ecosystems
Authors: Diez-Caballero Koldo , Troiteiro Silvia , García-Alba Javier , Vidal Juan Ramón , González Marta , Ametller Sergi , Juan Raquel
Published: June 2022

Parc Tramuntana is the first offshore wind project being promoted in the Catalonian waters, and due to this newness, it has generated a strong social debate surrounding expected environmental and socioeconomic impacts traditionally associated to marine wind farms, as there are no relevant references in this area. The objective of this report is to provide a specific analysis of some of the main potential impacts, based on detailed information and quantitative data, in order to place these impacts in a realistic context and determine their actual magnitude. This analysis is fed by diverse and detailed studies carried out over the last two years to assess the environmental impact of the project, in accordance with current regulations. According to environmental impact assessment, which is based on a standardized methodology, the impact of the project is objectively qualified as MODERATE on vectors such as turbidity and sedimentation, underwater noise, hydrodynamic circulation or the alteration of electromagnetic fields, and NOT SIGNIFICANT on aspects such as the proliferation of invasive exotic species. As this is an ongoing assessment process, this report presents initial conclusions that do not yet address all possible impacts. Nevertheless, the authors stress the importance of framing the debate on offshore wind in Catalonia in the context of the urgency of the climate emergency and its inevitable impacts on the natural environment.
Genetic Characterization of Microsporum canis Clinical Isolates in the United States
Authors: Moskaluk Alex , Darlington Lauren , Kuhn Sally , Behzadi Elisa , Gagne Roderick , Kozakiewicz Christopher , VandeWoude Sue
Published: June 2022

Microsporum canis is the primary agent causing dermatophytosis in cats, and also infects humans, dogs, and other species. Assessment of genetic variation among M. canis isolates in the United States has not been conducted. Further, M. canis mating type and assessment of disease severity associated with genotypic characteristics have not been rigorously evaluated. We therefore isolated M. canis from 191 domestic cats across the US and characterized genotypes by evaluation of ITS sequence, MAT locus, and microsatellite loci analysis. The genes SSU1 and SUB3, which are associated with keratin adhesion and digestion, were sequenced from a subset of isolates to evaluate potential genetic associations with virulence. Analysis of microsatellite makers revealed three M. canis genetic clusters. Both clinic location and disease severity were significant predictors of microsatellite variants. 100% of the M. canis isolates were MAT1-1 mating gene type, indicating that MAT1-2 is very rare or extinct in the US and that asexual reproduction is the dominant form of replication. No genetic variation at SSU1 and SUB3 was observed. These findings pave the way for novel testing modalities for M. canis and provide insights about transmission and ecology of this ubiquitous and relatively uncharacterized agent.
Comparative Fungicidal Activities of N-Chlorotaurine and Conventional Antiseptics against Candida spp. Isolated from Vulvovaginal Candidiasis
Authors: Hacioglu Mayram , Oyardi Ozlem , Yilmaz Fatima Nur , Nagl Markus
Published: June 2022

N-chlorotaurine (NCT), the N-chloro derivative of the amino acid taurine, is a long-lived oxidant produced by stimulated human leucocytes. NCT has antimicrobial activities which are generally enhanced in the presence of organic material. The aim of this study was to investigate fungicidal effects of NCT and conventional antiseptics against Candida isolated from vulvovaginal candidiasis (VVC). Chlorhexidine (CHX, 1.6%), octenidine dihydrochloride (OCT, 0.08%), povidone iodine (PVP-I, 8%), boric acid (8%), and NCT (0.1% (5.5 mM)) were evaluated against forty-four Candida isolates, according to European Standard methods, at 30, 60, 90, and 120 min and 24 h in the presence of skim milk as an organic material. CHX, OCT, and PVP-I showed rapid fungicidal activity against all Candida isolates with 5–6 log10 reduction of viable counts after 30 min, whereas boric acid and NCT needed 1 h against Candida albicans and 2 h against non-albicans Candida for a significant 3 log10 reduction. NCT showed fungicidal activity (defined as ≥4 log10 reduction) against C. albicans within 90 min and C. non–albicans within 24 h. Based upon all presently available data, including our results, NCT could be used as a new agent for treatment of local fungal infections such as VVC.
A Primer on Rules of Origin as Non-Tariff Barriers
Authors: Kniahin Dzmitry , de Melo Jaime
Published: June 2022

An explosion of different preferential rules of origin (PROO) has accompanied the spread of preferential trade agreements (PTAs) around the world. Complying with PROO requirements entail costs for producers, exporters, and customs officials. Observers, firms, customs officials, and policymakers have advocated simplification as well as harmonization. The paper surveys the literature drawing on the extensive database in ITC’s Rules of Origin Facilitator (ROF) database covering 54,000 distinct PROO spread across 370 PTAs to illustrate the issues covered in the literature. We review what we know about the compliance costs associated with PROO requirements. We illustrate these costs graphically and summarize through mathematical decomposition of compliance costs along two dimensions: distortionary costs resulting from the restrictiveness of PROOs and administrative costs. We survey the existing evidence in literature by themes: (i) determinants of the utilisation of preferences; (ii) effects on third countries outside the PTA; (iii) choice of rule; (iv) preference margin and complexity of rules; (v) trade deflection; and (vi) firm-level evidence. In conclusion, drawing lessons from the empirical literature is a complicated exercise because preference uptake, an important indicator of compliance costs, is only available for a handful of PTAs at the disaggregated product level.
Effect of Oxide Metallurgy on Inclusions in 125 ksi Grade OCTG Steel with Sulfide Stress Corrosion Resistance
Authors: Zhang Si , Li Yanmei , Wang Ping , Zhu Fuxian , Yang Yulong , Xiao Bang
Published: June 2022

The effects of Al deoxidation and Zr deoxidation on the microstructure and properties of sulfide stress corrosion resistant high-strength steel have been investigated. The feasibility of the Zr deoxidation instead of Al deoxidation was confirmed by the thermodynamic analysis of the deoxidation of various elements. The experimental results indicate that the average diameters of the inclusions in Al-Steel and Zr-Steel were 2.45 μm and 1.65 μm, respectively. The Al-Steel and Zr-Steel contained 22.38% and 68.77% inclusions per unit area, respectively, and the fraction of inclusions in the Al-Steel and Zr-Steel with diameters less than 2 μm was about 73.46% and 89.63%, respectively, indicating that the Zr deoxidation process could effectively refine inclusions and promote dispersion. The average diameters of austenite grain for the Al-Steel and Zr-Steel were about 9.1 μm and 8 μm, respectively. The fine particles in Zr-Steel could pin the austenite grain boundaries and clearly refine the grains. The average grain size of tempered martensite was 8.2 μm and 3.8 μm, respectively. The yield strength of the Al-Steel and Zr-Steel was 922 MPa and 939 MPa, respectively; the impact energy was 60 ± 6 J and 132 ± 6 J, respectively. Moreover, the fracture time of the NACE-A was from 28 h (Al-Steel) to 720 h (Zr-Steel) without fracture. The experimental steel deoxidized by Zr achieved a simultaneous improvement in strength, toughness and sulfide stress corrosion resistance, and the effect of inclusions on the fracture of the sulfide stress corrosion resistant high-strength steel can be explained by the Griffith theory.
Advanced Photocatalytic Treatment of Wastewater Using Immobilized Titanium Dioxide as a Photocatalyst in a Pilot-Scale Reactor: Process Intensification
Authors: Kane Abdoulaye , Assadi Achraf Amir , El Jery Atef , Badawi Ahmad K. , Kenfoud Hamza , Baaloudj Oussama , Assadi Aymen Amin
Published: June 2022

In many nations, particularly those experiencing water scarcity, novel approaches are being applied to clean wastewater. Heterogeneous photocatalysis is the most widely used of these approaches because it entails the decomposition of organic molecules into water and carbon dioxide, which is a more ecologically benign process. In our study, we studied the photocatalytic degradation process on the effluent flumequine. This treatment is made through a solar pilot reactor in the presence of immobilized titanium dioxide with three light intensities and two types of water as solvents. A variety of factors that might influence the rate of deterioration, such as flow rate, light intensity, and initial concentration, have been investigated. The maximal degradation of flumequine was achieved at more than 90% after 2.5 h under optimal conditions (an initial concentration of 5 mg/L, three lamp light intensities, and a flow rate of 29 L/h). By combining the oxidized agent H2O2 with this process, the photocatalytic activity was improved further to 97% under the same conditions. The mineralization of this product has also been tested using total organic carbon (TOC) analysis. A high mineralization rate has been recorded at around 50% for a high initial concentration (20 mg/L) at a flow rate of 126 L/h. The results demonstrated the highly effective removal of flumequine and the efficacy of this photocatalytic system.
Special Issue “Design and Application of Additive Manufacturing”
Authors: Paz Rubén
Published: June 2022

Additive manufacturing (AM) is continuously improving and offering new opportunities in the manufacturing industry [...]
The Feature Extraction of Impact Response and Load Reconstruction Based on Impulse Response Theory
Authors: Huang Dawei , Gao Yadong , Yu Xinyu , Chen Likun
Published: June 2022

Impact load is a kind of aperiodic excitation with a short action time and large amplitude, it had more significant effect on the structure than static load. The reconstruction (or identification namely) of impact load is of great importance for validating the structural strength. The aim of this article was to reconstruct the impact load accurately. An impact load identification method based on impulse response theory (IRT) and BP (Back Propagation) neural network is proposed. The excitation and response signals were transformed to the same length by extracting the peak value (amplitude of sine wave) in the rising oscillation period of the response. First, we deduced that there was an approximate linear relationship between the discrete-time integral of impact load and the amplitude of the oscillation period of the response. Secondly, a BP neural network was used to establish a linear relationship between the discrete-time integral of the impact load and the peak value in the rising oscillation period of the response. Thirdly, the network was trained and verified. The error between the actual maximum amplitude of impact load and the identification value was 2.22%. The error between the actual equivalent impulse and the identification value was 0.67%. The results showed that this method had high accuracy and application potential.
Fault Detection for High-Speed Trains Using CCA and Just-in-Time Learning
Authors: Zheng Hong , Zhu Keyuan , Cheng Chao , Fu Zhaowang
Published: June 2022

Online monitors of the running gears systems of high-speed trains play critical roles in ensuring operational safety and reliability. Status signals collected from high-speed train running gears are very complex regarding working environments, random noises and many other real-world constraints. This paper proposed fault detection (FD) models using canonical correlation analysis (CCA) and just-in-time learning (JITL) to process scalar signals of high-speed train gears, named as CCA-JITL. After data preprocessing and normalization, CCA transforms covariance matrices of high-dimension historical data into low-dimension subspaces and maximizes correlations between the most important latent dimensions. Then, JITL components formulate local FD models which utilize subsets of testing samples with larger Euclidean distances to training data. A case study introduced a novel system design of an online FD architecture and demonstrated that CCA-JITL FD models significantly outperformed traditional CCA models. The approach is applicable to other dimension reduction FD models such as PCA and PLS.