bluuebunny/crossref_metadata_2025 huggingface dataset:
Geothermal Linkage between a Hydrothermal Pond and a Deep Lake: Kuttara Volcano, Japan
Authors: Chikita Kazuhisa , Ochiai Yasuhiro , Oyagi Hideo , Sakata Yoshitaka
Published: January 2019

Kuttara Volcano, Hokkaido, Japan, consists of temperate Lake Kuttara and the western Noboribetsu geothermal area. In order to explore geothermal relations between Lake Kuttara and the geothermal area, the heat budget of a hydrothermal pond, Okunoyu, was evaluated, and the heat storage change in the lower layer of Lake Kuttara was calculated by monitoring the water temperature at the deepest point. The lake water temperature consistently increased during the thermal stratification in June–November of 2013–2016. The heat flux QB at lake bottom was then calculated at a range of 4.1–10.9 W/m2, which is probably due to the leakage from a hydrothermal reservoir below the lake bottom. Meanwhile, the heat flux HGin by geothermal groundwater input in Okunoyu was evaluated at 3.5–8.5 kW/m2, which is rapidly supplied through faults from underlying hydrothermal reservoirs. With a time lag of 5 months to monthly mean QB values in Lake Kuttara, the correlation with monthly mean HGin in Okunoyu was significant (R2 = 0.586; p < 0.01). Applying Darcy’s law to the leakage from the hydrothermal reservoir at 260–310 m below the lake bottom, the time needed for groundwater’s passage through the media 260–310 m thick was evaluated at 148–149 days (ca. 5 months). These findings suggest that the hydrothermal reservoir below lake bottom and the underlying hydrothermal reservoirs in the western geothermal area are both connected to a unique geothermal source in the deeper zone as a geothermal flow system of Kuttara Volcano.
Consumer’s Willingness to Pay a Premium for Organic Fruits in China: A Double-Hurdle Analysis
Authors: Wang Lijia , Wang Jianhua , Huo Xuexi
Published: January 2019

The aim of the paper was to assess how consumers evaluate organic labeled fruits and to what extent they are willing to pay a premium for fresh fruits with organic labels. A double-hurdle model is applied to data obtained by interviewing 407 fresh fruit consumers in nine Chinese cities. Willingness-to-pay a premium was modeled as a function of a series of demographic, socio-economic variables, plus fruit attributes, perceptions of fruit safety, and risk attitudes. Results indicate that the most important factors influencing willingness to pay a premium involved positive attitudes toward organic label, attention to fruit safety, the perception of importance of fruit attributes. Moreover, the more income consumers earn, the more likely they would be willing to pay a premium for organic fresh fruits. The recorded consumer interest in safety and quality of fresh fruits reveals that a promising market for organic fruits could be developed by an adequate knowledge on organic label and an effective market monitoring system.
Quantitative Measures of Physical Risk Factors Associated with Work-Related Musculoskeletal Disorders of the Elbow: A Systematic Review
Authors: Seidel David H. , Ditchen Dirk M. , Hoehne-Hückstädt Ulrike M. , Rieger Monika A. , Steinhilber Benjamin
Published: January 2019

Background: Work-related musculoskeletal disorders at the elbow are a common health problem, which highly impacts workers’ well-being and performance. Besides existing qualitative information, there is a clear lack of quantitative information of physical risk factors associated with specific disorders at the elbow (SDEs). Objective: To provide evidence-based quantitative measures of physical risk factors associated with SDEs. Methods: Studies were searched from 2007 to 2017 in Medline, EMBASE, and Cochrane Work. The identified risk factors were grouped in main- and sub-categories of exposure using the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) framework for rating evidence. Results: 133 different risk-factor specifications were identified in 10/524 articles and were grouped into 5 main- and 16 sub-categories of exposure. The risk factors were significantly associated with lateral epicondylitis, medial epicondylitis, or ulnar neuropathy. Significant risk factors such as wrist angular velocity (5°/s, with increasing prevalence ratio of 0.10%/(°/s), or forearm supination (≥45° and ≥5% of time combined with forceful lifting) were found. Conclusions: This review delivers a categorization of work-related physical risk-factor specifications for SDEs with a special focus on quantitative measures, ranked for evidence. These results may build the base for developing risk assessment methods and prospective preventive measures.
Action 3:30R: Results of a Cluster Randomised Feasibility Study of a Revised Teaching Assistant-Led Extracurricular Physical Activity Intervention for 8 to 10 Year Olds
Authors: Jago Russell , Tibbitts Byron , Sanderson Emily , Bird Emma L. , Porter Alice , Metcalfe Chris , Powell Jane E. , Gillett Darren , Sebire Simon J.
Published: January 2019

Many children are not sufficiently physically active. We conducted a cluster-randomised feasibility trial of a revised after-school physical activity (PA) programme delivered by trained teaching assistants (TAs) to assess the potential evidence of promise for increasing moderate-to-vigorous physical activity (MVPA). Participants (n = 335) aged 8–10 years were recruited from 12 primary schools in South West England. Six schools were randomised to receive the intervention and six acted as non-intervention controls. In intervention schools, TAs were trained to deliver an after-school programme for 15 weeks. The difference in mean accelerometer-assessed MVPA between intervention and control schools was assessed at follow-up (T1). The cost of programme delivery was estimated. Two schools did not deliver the intervention, meaning four intervention and six control schools were analysed at T1. There was no evidence for a difference in MVPA at T1 between intervention and control groups. Programme delivery cost was estimated at £2.06 per pupil per session. Existing provision in the 12 schools cost £5.91 per pupil per session. Action 3:30 was feasible to deliver and considerably cheaper than existing after-school provision. No difference in weekday MVPA was observed at T1 between the two groups, thus progression to a full trial is not warranted.
Breastfeeding and the Risk of Illness among Young Children in Rural China
Authors: Li Shanshan , Yue Ai , Abbey Cody , Medina Alexis , Shi Yaojiang
Published: January 2019

Poor rural areas in China exhibit the country’s highest rates of child mortality, often stemming from preventable health conditions such as diarrhea and respiratory infection. In this study, we investigate the association between breastfeeding and disease among children aged 6–24 months in poor rural counties in China. To do this, we conducted a longitudinal, quantitative analysis of socioeconomic demographics, health outcomes, and breastfeeding practices for 1802 child–caregiver dyads across 11 nationally designated poverty counties in southern Shaanxi Province in 2013–2014. We found low rates of continued breastfeeding that decreased as children developed: from 58.2% at 6–12 months, to 21.6% at 12–18 months, and finally to 5.2% at 18–24 months. These suboptimal rates are lower than all but one other country in the Asia-Pacific region. We further found that only 18.3% of children 6–12 months old met the World Health Organization (WHO)-recommended threshold for minimum dietary diversity, defined as consuming four or more of seven specific food groups. Breastfeeding was strongly associated with lower rates of both diarrhea and cough in bivariate and multivariate analyses. As the first analysis to use longitudinal data to examine the relationship between continued breastfeeding and child illness in China, our study confirms the need for programmatic interventions that promote continued breastfeeding in order to improve toddler health in the region.
Spatiotemporal Variations of Indoor PM2.5 Concentrations in Nanjing, China
Authors: Shao Zhijuan , Yin Xiangjun , Bi Jun , Ma Zongwei , Wang Jinnan
Published: January 2019

Indoor fine particulate matter (PM2.5) is important since people spend most of their time indoors. However, knowledge of the spatiotemporal variations of indoor PM2.5 concentrations within a city is limited. In this study, the spatiotemporal distributions of indoor PM2.5 levels in Nanjing, China were modeled by the multizone airflow and contaminant transport program (CONTAM), based on the geographically distributed residences, human activities, and outdoor PM2.5 concentrations. The accuracy of the CONTAM model was verified, with a good agreement between the model simulations and measurements (r = 0.940, N = 110). Two different scenarios were considered to examine the building performance and influence of occupant behaviors. Higher PM2.5 concentrations were observed under the scenario when indoor activities were considered. Seasonal variability was observed in indoor PM2.5 levels, with the highest concentrations occurring in the winter and the lowest occurring in the summer. Building characteristics have a significant effect on the spatial distribution of indoor PM2.5 concentrations, with multistory residences being more vulnerable to outdoor PM2.5 infiltration than high-rise residences. The overall population exposure to PM2.5 in Nanjing was estimated. It would be overestimated by 16.67% if indoor exposure was not taken into account, which would lead to a bias in the health impacts assessment.
Assessing the Ecological Effects of Water Transport to a Lake in Arid Regions: A Case Study of Qingtu Lake in Shiyang River Basin, Northwest China
Authors: Chunyu Xunzhou , Huang Feng , Xia Ziqiang , Zhang Danrong , Chen Xi , Xie Yongyu
Published: January 2019

With the continuous growth of economic water consumption in arid regions, many endorheic rivers and terminal lakes have desiccated. As an important ecological engineering measure, water transport in arid regions has vital ecological significance for protecting the regional ecological environment and delaying desertification. In this study, Qingtu Lake, the terminal lake of Shiyang River, was selected to analyze the ecological effects of water transport by means of remote sensing interpretations and current year field investigations. The results demonstrated that, in July 2018, the water surface had formed and recovered to 5.68 km2. Additionally, Qingtu Lake formed a spatial gradient distribution in groundwater depth. The depth increased in gradient from the waterside to the desert edge. There was a significant increase in the overall regional vegetation coverage, which mainly occurred in the water areas because of the extensive growth in Phragmites australis, which reached 10.54 km2 in area in 2018. Furthermore, the regional vegetation formed a gradient distribution, which transitioned from hygrophytes to xerophytes. This study can provide guidelines for the protection and restoration of lakes in arid regions.
Bisphenol A Exposure and Sperm ACHE Hydroxymethylation in Men
Authors: Song Xiuxia , Miao Maohua , Zhou Xiaoyu , Li Dekun , Tian Youping , Liang Hong , Li Runsheng , Yuan Wei
Published: January 2019

Exposure to bisphenol A (BPA) has been shown to impact human sperm quality. The epigenetic mechanisms underlying the effect remain unknown. The acetylcholinesterase (ACHE) gene is a sperm-expressed gene encoding the acetylcholine hydrolyzing enzyme acetylcholinesterase and participates in the apoptosis of cells, including sperm. This study aimed to examine whether BPA exposure is associated with the hydroxymethylation level of the sperm ACHE gene. A total of 157 male factory workers were studied, among whom 74 had BPA exposure in the workplace (BPA exposure group) and 83 had no BPA exposure in the workplace (control group). Urine samples were collected for BPA measurement and semen samples were collected to assay for ACHE hydroxymethylation. Sperm ACHE hydroxymethylation level was higher in the BPA exposure group (p = 0.041) compared to the control group. When subjects were categorized according to tertiles of detected BPA level, higher ACHE hydroxymethylation levels were observed for the lowest, middle, and top tertiles compared to those with BPA below the limit of detection (LOD). In a linear regression analysis adjusted for confounders, a positive linear association between urine BPA concentration and 5-hydroxymethylcytosine (5hmC) rate of the sperm ACHE gene was observed, although the association did not reach statistical significance in all categories after being stratified by the BPA tertile. In conclusion, 5hmC of the sperm ACHE gene was positively associated with BPA exposure, which may provide supportive evidence for BPA’s effects on male fertility or other health endpoints.
Use of a Modified DANP-mV Model to Improve Quality of Life in Rural Residents: The Empirical Case of Xingshisi Village, China
Authors: Qu Guang-Bin , Zhao Tian-Yu , Zhu Bo-Wei , Tzeng Gwo-Hshiung , Huang Shan-Lin
Published: January 2019

Climate change-related anomalies have increased public concern regarding environmental protection. This has opened newer rural development avenues. In this regard, livability of villages is crucial; it can be evaluated based on the villagers’ quality of life (QoL). The WHOQOL-BREF, a comprehensive cross-cultural and cross-disciplinary scale proposed by the World Health Organization to assess QoL, has aided in assessing and improving QoL in different regions. However, the factors of this instrument are mutually influential, necessitating an improvement strategy considering the entire system. This problem may be resolved using the DANP-mV model. However, the traditional DANP-mV model includes many items and responding to all of them is difficult for experts. Therefore, by using the case of Xingshisi Village in China, this study proposed a modified DANP-mV model to provide additional suggestions for systematic improvement of the QoL and livability in the village. Xingshisi is a model village built according to an aspirational benchmark; however, different from the traditional definition of a benchmark, this village exhibits room for improvement. Although the modified model reduces the number of questions from 650 to 168, its effect remains similar to that of the traditional model. Moreover, in the modified model, physical capacity (D1) presented the largest dimensional gap. The interaction among the factors indicated that considering the effect of the environment (D4) and developing a systematic improvement strategy are necessary to improve the livability of villages facing limited resources.
Investigation of the Contributory Factors to the Guessability of Traffic Signs
Authors: Liu Jing , Wen Huiying , Zhu Dianchen , Kumfer Wesley
Published: January 2019

Traffic signs play an important role in traffic management systems. A variety of studies have focused on drivers’ comprehension of traffic signs. However, the travel safety of prospective users, which has been rarely mentioned in previous studies, has attracted considerable attention from relevant departments in China. With the growth of international and interregional travel demand, traffic signs should be designed more universally to reduce the potential risks to drivers. To identify key factors that improve prospective users’ sign comprehension, this study investigated eight factors that may affect users’ performance regarding sign guessing. Two hundred and one Chinese students, all of whom intended to be drivers and none of whom had experience with daily driving after obtaining a license or visits to Germany, guessed the meanings and rated the sign features of 54 signs. We investigated the effects of selected user factors on their sign guessing performance. Additionally, the contributions of four cognitive design features to the guessability of traffic signs were examined. Based on an analysis of the relationships between the cognitive features and the guessability score of signs, the contributions of four sign features to the guessability of traffic signs were examined. Moreover, by exploring Chinese users’ differences in guessing performance between Chinese signs and German signs, cultural issues in sign design were identified. The results showed that vehicle ownership and attention to traffic signs exerted a significant influence on guessing performance. As expected, driver’s license training and the number of years in college were dominant factors for guessing performance. With regard to design features, semantic distance and confidence in guessing were two dominant factors for the guessability of signs. We suggest improving the design of signs by including vivid, universal symbols. Thus, we provide several suggestions for designing more user-friendly signs.
Cap-Independent mRNA Translation in Germ Cells
Authors: Keiper Brett D.
Published: January 2019

Cellular mRNAs in plants and animals have a 5′-cap structure that is accepted as the recognition point to initiate translation by ribosomes. Consequently, it was long assumed that the translation initiation apparatus was built solely for a cap-dependent (CD) mechanism. Exceptions that emerged invoke structural damage (proteolytic cleavage) to eukaryotic initiation factor 4 (eIF4) factors that disable cap recognition. The residual eIF4 complex is thought to be crippled, but capable of cap-independent (CI) translation to recruit viral or death-associated mRNAs begrudgingly when cells are in great distress. However, situations where CI translation coexists with CD translation are now known. In such cases, CI translation is still a minor mechanism in the major background of CD synthesis. In this review, I propose that germ cells do not fit this mold. Using observations from various animal models of oogenesis and spermatogenesis, I suggest that CI translation is a robust partner to CD translation to carry out the translational control that is so prevalent in germ cell development. Evidence suggests that CI translation provides surveillance of germ cell homeostasis, while CD translation governs the regulated protein synthesis that ushers these meiotic cells through the remarkable steps in sperm/oocyte differentiation.
Divergence in Glucosinolate Profiles between High- and Low-Elevation Populations of Arabidopsis halleri Correspond to Variation in Field Herbivory and Herbivore Behavioral Preferences
Authors: Buckley James , Pashalidou Foteini G. , Fischer Martin C. , Widmer Alex , Mescher Mark C. , De Moraes Consuelo M.
Published: January 2019

Variation in local herbivore pressure along elevation gradients is predicted to drive variation in plant defense traits. Yet, the extent of intraspecific variation in defense investment along elevation gradients, and its effects on both herbivore preference and performance, remain relatively unexplored. Using populations of Arabidopsis halleri (Brassicaceae) occurring at different elevations in the Alps, we tested for associations between elevation, herbivore damage in the field, and constitutive chemical defense traits (glucosinolates) assayed under common-garden conditions. Additionally, we examined the feeding preferences and performance of a specialist herbivore, the butterfly Pieris brassicae, on plants from different elevations in the Alps. Although we found no effect of elevation on the overall levels of constitutive glucosinolates in leaves, relative amounts of indole glucosinolates increased significantly with elevation and were negatively correlated with herbivore damage in the field. In oviposition preference assays, P. brassicae females laid fewer eggs on plants from high-elevation populations, although larval performance was similar on populations from different elevations. Taken together, these results support the prediction that species distributed along elevation gradients exhibit genetic variation in chemical defenses, which can have consequences for interactions with herbivores in the field.
In Situ Monitoring of Bacteria under Antimicrobial Stress Using 31P Solid-State NMR
Authors: Overall Sarah A. , Zhu Shiying , Hanssen Eric , Separovic Frances , Sani Marc-Antoine
Published: January 2019

In-cell NMR offers great insight into the characterization of the effect of toxins and antimicrobial peptides on intact cells. However, the complexity of intact live cells remains a significant challenge for the analysis of the effect these agents have on different cellular components. Here we show that 31P solid-state NMR can be used to quantitatively characterize the dynamic behaviour of DNA within intact live bacteria. Lipids were also identified and monitored, although 31P dynamic filtering methods indicated a range of dynamic states for phospholipid headgroups. We demonstrate the usefulness of this methodology for monitoring the activity of the antibiotic ampicillin and the antimicrobial peptide (AMP) maculatin 1.1 (Mac1.1) against Gram-negative bacteria. Perturbations in the dynamic behaviour of DNA were observed in treated cells, which indicated additional mechanisms of action for the AMP Mac1.1 not previously reported. This work highlights the value of 31P in-cell solid-state NMR as a tool for assessing the antimicrobial activity of antibiotics and AMPs in bacterial cells.
Kinetics and Predicted Structure of a Novel Xylose Reductase from Chaetomium thermophilum
Authors: Quehenberger Julian , Reichenbach Tom , Baumann Niklas , Rettenbacher Lukas , Divne Christina , Spadiut Oliver
Published: January 2019

While in search of an enzyme for the conversion of xylose to xylitol at elevated temperatures, a xylose reductase (XR) gene was identified in the genome of the thermophilic fungus Chaetomium thermophilum. The gene was heterologously expressed in Escherichia coli as a His6-tagged fusion protein and characterized for function and structure. The enzyme exhibits dual cofactor specificity for NADPH and NADH and prefers D-xylose over other pentoses and investigated hexoses. A homology model based on a XR from Candida tenuis was generated and the architecture of the cofactor binding site was investigated in detail. Despite the outstanding thermophilicity of its host the enzyme is, however, not thermostable.
New Therapeutic Implications of Endothelial Nitric Oxide Synthase (eNOS) Function/Dysfunction in Cardiovascular Disease
Authors: Daiber Andreas , Xia Ning , Steven Sebastian , Oelze Matthias , Hanf Alina , Kröller-Schön Swenja , Münzel Thomas , Li Huige
Published: January 2019

The Global Burden of Disease Study identified cardiovascular risk factors as leading causes of global deaths and life years lost. Endothelial dysfunction represents a pathomechanism that is associated with most of these risk factors and stressors, and represents an early (subclinical) marker/predictor of atherosclerosis. Oxidative stress is a trigger of endothelial dysfunction and it is a hall-mark of cardiovascular diseases and of the risk factors/stressors that are responsible for their initiation. Endothelial function is largely based on endothelial nitric oxide synthase (eNOS) function and activity. Likewise, oxidative stress can lead to the loss of eNOS activity or even “uncoupling” of the enzyme by adverse regulation of well-defined “redox switches” in eNOS itself or up-/down-stream signaling molecules. Of note, not only eNOS function and activity in the endothelium are essential for vascular integrity and homeostasis, but also eNOS in perivascular adipose tissue plays an important role for these processes. Accordingly, eNOS protein represents an attractive therapeutic target that, so far, was not pharmacologically exploited. With our present work, we want to provide an overview on recent advances and future therapeutic strategies that could be used to target eNOS activity and function in cardiovascular (and other) diseases, including life style changes and epigenetic modulations. We highlight the redox-regulatory mechanisms in eNOS function and up- and down-stream signaling pathways (e.g., tetrahydrobiopterin metabolism and soluble guanylyl cyclase/cGMP pathway) and their potential pharmacological exploitation.
Zhile Capsule Exerts Antidepressant-Like Effects through Upregulation of the BDNF Signaling Pathway and Neuroprotection
Authors: Wu Jiawei , Zhang Tingting , Yu Luping , Huang Shuai , Yang Yu , Yu Suyun , Li Jun , Cao Yuzhu , Wei Zhonghong , Li Xiaoman , Wu Yuanyuan , Chen Wenxing , Wang Aiyun , Lu Yin
Published: January 2019

Major depressive disorder is now becoming a common disease in daily life, and most patients do not have satisfactory treatment outcomes. We herein evaluated the therapeutic effects of Zhile capsule and clarified the molecular mechanism. A rat model of chronic unpredictable mild stress-induced depression was established to assess the antidepressant-like effects of Zhile by using the sucrose preference test, open field test, forced swim test, tail suspension test and HPLC. Systems pharmacology was then performed to unravel the underlying mechanism which was confirmed by western blot, enzyme-linked immunosorbent assay, and qPCR. Zhile alleviated depression-like behaviors by upregulating the cAMP-CREB-BDNF (brain-derived neurotrophic factor) axis to exert neuroprotective effects. It may be beneficial to depressive patients in clinical practice.
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement
Authors: Foulon Valentin , Boudry Pierre , Artigaud Sébastien , Guérard Fabienne , Hellio Claire
Published: January 2019

Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the pediveliger stage. Our analysis revealed 59 sequences potentially implicated in adhesion of C. gigas larvae. Some related proteins contain conserved domains already described in other bioadhesives. We propose a hypothetic composition of C. gigas bioadhesive in which the protein constituent is probably composed of collagen and the von Willebrand Factor domain could play a role in adhesive cohesion. Genes coding for enzymes implicated in DOPA chemistry were also detected, indicating that this modification is also potentially present in the adhesive of pediveliger larvae.
Targeting Telomerase and ATRX/DAXX Inducing Tumor Senescence and Apoptosis in the Malignant Glioma
Authors: Fan Hueng-Chuen , Chen Chuan-Mu , Chi Ching-Shiang , Tsai Jeng-Dau , Chiang Kuo-Liang , Chang Yu-Kang , Lin Shinn-Zong , Harn Horng-Jyh
Published: January 2019

Glioblastoma multiforme (GBM) is a type of brain tumor that is notorious for its aggressiveness and invasiveness, and the complete removal of GBM is still not possible, even with advanced diagnostic strategies and extensive therapeutic plans. Its dismal prognosis and short survival time after diagnosis make it a crucial public health issue. Understanding the molecular mechanisms underlying GBM may inspire novel and effective treatments against this type of cancer. At a molecular level, almost all tumor cells exhibit telomerase activity (TA), which is a major means by which they achieve immortalization. Further studies show that promoter mutations are associated with increased TA and stable telomere length. Moreover, some tumors and immortalized cells maintain their telomeres with a telomerase-independent mechanism termed the “alternative lengthening of telomeres” (ALT), which relates to the mutations of the α-thalassemia/mental retardation syndrome X-linked protein (ATRX), the death-domain associated protein (DAXX) and H3.3. By means of the mutations of the telomerase reverse transcriptase (TERT) promoter and ATRX/DAXX, cancers can immortalize and escape cell senescence and apoptosis. In this article, we review the evidence for triggering GBM cell death by targeting telomerase and the ALT pathway, with an extra focus on a plant-derived compound, butylidene phthalide (BP), which may be a promising novel anticancer compound with good potential for clinical applications.
Chromatographic Separation of Phenolic Compounds from Extra Virgin Olive Oil: Development and Validation of a New Method Based on a Biphenyl HPLC Column
Authors: Ferro Miguel D. , Santos Sónia A. O. , Silvestre Armando J. D. , Duarte Maria F.
Published: January 2019

Three different high performance liquid chromatography columns were accessed for phenolic compounds (PC) separation in the hydrophilic fraction of extra virgin olive oil (EVOO). Two fully porous C18 bonded silica phases and one partially porous biphenyl column were used. Biphenyl column allowed for an increase of more than 30% in peak capacity (nc), higher selectivity (α) (1.045), and improved retention (k), with a reduction of 22.1% in the retention time. The higher resolution (Rs) was obtained by using the biphenyl column, with a fair separation of oleuropein aglycone isomers (OAI) and a good identification of caffeic acid (CA). Tyrosol (T), hydroxytyrosol (HT), and dihydroxyphenyl glycol (DHPG) were also well separated and identified. Moreover, the method using a biphenyl column was fully validated according to the requirements for new methods. For all parameters, the method applying the biphenyl column proved to be a reliable, accurate, and robust tool for separation, identification, and quantification of the main PCs in EVOOs.
Hepatotoxicity: Molecular Mechanisms and Pathophysiology
Authors: Teschke Rolf
Published: January 2019

The current Special Issue is devoted to the broad spectrum of hepatotoxicity with its molecular mechanisms and pathophysiology, presented in eight publications [...]
Cathepsin L-like Cysteine Proteinase Genes Are Associated with the Development and Pathogenicity of Pine Wood Nematode, Bursaphelenchus xylophilus
Authors: Xue Qi , Wu Xiao-Qin , Zhang Wan-Jun , Deng Li-Na , Wu Miao-Miao
Published: January 2019

The pine wood nematode (PWN), Bursaphelenchus xylophilus, is the pathogen of pine wilt disease (PWD), resulting in huge losses in pine forests. However, its pathogenic mechanism remains unclear. The cathepsin L-like cysteine proteinase (CPL) genes are multifunctional genes related to the parasitic abilities of plant-parasitic nematodes, but their functions in PWN remain unclear. We cloned three cpl genes of PWN (Bx-cpls) by rapid amplification of cDNA ends (RACE) and analyzed their characteristics using bioinformatic methods. The tissue specificity of cpl gene of PWN (Bx-cpl) was studied using in situ mRNA hybridization (ISH). The functions of Bx-cpls in development and pathogenicity were investigated using real-time quantitative PCR (qPCR) and RNA interference (RNAi). The results showed that the full-length cDNAs of Bx-cpl-1, Bx-cpl-2, and Bx-cpl-3 were 1163 bp, 1305 bp, and 1302 bp, respectively. Bx-cpls could accumulate specifically in the egg, intestine, and genital system of PWN. During different developmental stages of PWN, the expression of Bx-cpls in the egg stage was highest. After infection, the expression levels of Bx-cpls increased and reached their highest at the initial stage of PWD, then declined gradually. The silencing of Bx-cpl could reduce the feeding, reproduction, and pathogenicity of PWN. These results revealed that Bx-cpls play multiple roles in the development and pathogenic processes of PWN.
Evaluation of the Biological Behavior of a Gold Nanocore-Encapsulated Human Serum Albumin Nanoparticle (Au@HSANP) in a CT-26 Tumor/Ascites Mouse Model after Intravenous/Intraperitoneal Administration
Authors: Chen Chao-Cheng , Li Jia-Je , Guo Nai-Hua , Chang Deng-Yuan , Wang Chung-Yih , Chen Jenn-Tzong , Lin Wuu-Jyh , Chi Kwan-Hwa , Lee Yi-Jang , Liu Ren-Shyan , Chen Chuan-Lin , Wang Hsin-Ell
Published: January 2019

Colorectal cancer is one of the major causes of cancer-related death in Taiwan and worldwide. Patients with peritoneal metastasis from colorectal cancer have reduced overall survival and poor prognosis. Hybrid protein-inorganic nanoparticle systems have displayed multifunctional applications in solid cancer theranostics. In this study, a gold nanocore-encapsulated human serum albumin nanoparticle (Au@HSANP), which is a hybrid protein-inorganic nanoparticle, and its radioactive surrogate 111In-labeled Au@HSANP (111In-Au@HSANP), were developed and their biological behaviors were investigated in a tumor/ascites mouse model. 111In-Au@HSANP was injected either intravenously (iv) or intraperitoneally (ip) in CT-26 tumor/ascites-bearing mice. After ip injection, a remarkable and sustained radioactivity retention in the abdomen was noticed, based on microSPECT images. After iv injection, however, most of the radioactivity was accumulated in the mononuclear phagocyte system. The results of biodistribution indicated that ip administration was significantly more effective in increasing intraperitoneal concentration and tumor accumulation than iv administration. The ratios of area under the curve (AUC) of the ascites and tumors in the ip-injected group to those in the iv-injected group was 93 and 20, respectively. This study demonstrated that the ip injection route would be a better approach than iv injections for applying gold-albumin nanoparticle in peritoneal metastasis treatment.
Vitamin D Supplementation in Central Nervous System Demyelinating Disease—Enough Is Enough
Authors: Häusler Darius , Weber Martin S.
Published: January 2019

The exact cause of multiple sclerosis (MS) remains elusive. Various factors, however, have been identified that increase an individual’s risk of developing this central nervous system (CNS) demyelinating disease and are associated with an acceleration in disease severity. Besides genetic determinants, environmental factors are now established that influence MS, which is of enormous interest, as some of these contributing factors are relatively easy to change. In this regard, a low vitamin D status is associated with an elevated relapse frequency and worsened disease course in patients with MS. The most important question, however, is whether this association is causal or related. That supplementing vitamin D in MS is of direct therapeutic benefit, is still a matter of debate. In this manuscript, we first review the potentially immune modulating mechanisms of vitamin D, followed by a summary of current and ongoing clinical trials intended to assess whether vitamin D supplementation positively influences the outcome of MS. Furthermore, we provide emerging evidence that excessive vitamin D treatment via the T cell-stimulating effect of secondary hypercalcemia, could have negative effects in CNS demyelinating disease. This jointly merges into the balancing concept of a therapeutic window of vitamin D in MS.
Physiological Analysis and Proteome Quantification of Alligator Weed Stems in Response to Potassium Deficiency Stress
Authors: Li Li-Qin , Lyu Cheng-Cheng , Li Jia-Hao , Tong Zhu , Lu Yi-Fei , Wang Xi-Yao , Ni Su , Yang Shi-Min , Zeng Fu-Chun , Lu Li-Ming
Published: January 2019

The macronutrient potassium is essential to plant growth, development and stress response. Alligator weed (Alternanthera philoxeroides) has a high tolerance to potassium deficiency (LK) stress. The stem is the primary organ responsible for transporting molecules from the underground root system to the aboveground parts of the plant. However, proteomic changes in response to LK stress are largely unknown in alligator weed stems. In this study, we investigated the physiological and proteomic changes in alligator weed stems under LK stress. First, the chlorophyll and soluble protein content and SOD and POD activity were significantly altered after 15 days of LK treatment. The quantitative proteomic analysis suggested that a total of 296 proteins were differentially abundant proteins (DAPs). The functional annotation analysis revealed that LK stress elicited complex proteomic alterations that were involved in oxidative phosphorylation, plant-pathogen interactions, glycolysis/gluconeogenesis, sugar metabolism, and transport in stems. The subcellular locations analysis suggested 104 proteins showed chloroplastic localization, 81 proteins showed cytoplasmic localization and 40 showed nuclear localization. The protein–protein interaction analysis revealed that 56 proteins were involved in the interaction network, including 9 proteins involved in the ribosome network and 9 in the oxidative phosphorylation network. Additionally, the expressed changes of 5 DAPs were similar between the proteomic quantification analysis and the PRM-MS analysis, and the expression levels of eight genes that encode DAPs were further verified using an RT-qPCR analysis. These results provide valuable information on the adaptive mechanisms in alligator weed stems under LK stress and facilitate the development of efficient strategies for genetically engineering potassium-tolerant crops.
Integration Analysis of Small RNA and Degradome Sequencing Reveals MicroRNAs Responsive to Dickeya zeae in Resistant Rice
Authors: Li Wenqi , Jia Yulin , Liu Fengquan , Wang Fangquan , Fan Fangjun , Wang Jun , Zhu Jinyan , Xu Yang , Zhong Weigong , Yang Jie
Published: January 2019

Rice foot rot disease caused by the pathogen Dickeya zeae (formerly known as Erwinia chrysanthemi pv. zeae), is a newly emerging damaging bacterial disease in China and the southeast of Asia, resulting in the loss of yield and grain quality. However, the genetic resistance mechanisms mediated by miRNAs to D. zeae are unclear in rice. In the present study, 652 miRNAs including osa-miR396f predicted to be involved in multiple defense responses to D. zeae were identified with RNA sequencing. A total of 79 differentially expressed miRNAs were detected under the criterion of normalized reads ≥10, including 51 known and 28 novel miRNAs. Degradome sequencing identified 799 targets predicted to be cleaved by 168 identified miRNAs. Among them, 29 differentially expressed miRNA and target pairs including miRNA396f-OsGRFs were identified by co-expression analysis. Overexpression of the osa-miR396f precursor in a susceptible rice variety showed enhanced resistance to D. zeae, coupled with significant accumulation of transcripts of osa-miR396f and reduction of its target the Growth-Regulating Factors (OsGRFs). Taken together, these findings suggest that miRNA and targets including miR396f-OsGRFs have a role in resisting the infections by bacteria D. zeae.
Newborn Screening for Sickle Cell Disease in the Caribbean: An Update of the Present Situation and of the Disease Prevalence
Authors: Knight-Madden Jennifer , Lee Ketty , Elana Gisèle , Elenga Narcisse , Marcheco-Teruel Beatriz , Keshi Ngozi , Etienne-Julan Maryse , King Lesley , Asnani Monika , Romana Marc , Hardy-Dessources Marie-Dominique
Published: January 2019

The region surrounding the Caribbean Sea is predominantly composed of island nations for its Eastern part and the American continental coast on its Western part. A large proportion of the population, particularly in the Caribbean islands, traces its ancestry to Africa as a consequence of the Atlantic slave trade during the XVI–XVIII centuries. As a result, sickle cell disease has been largely introduced in the region. Some Caribbean countries and/or territories, such as Jamaica and the French territories, initiated newborn screening (NBS) programs for sickle cell disease more than 20 years ago. They have demonstrated the major beneficial impact on mortality and morbidity resulting from early childhood care. However, similar programs have not been implemented in much of the region. This paper presents an update of the existing NBS programs and the prevalence of sickle cell disease in the Caribbean. It demonstrates the impact of the Caribbean Network of Researchers on Sickle Cell Disease and Thalassemia (CAREST) on the extension of these programs. The presented data illustrate the importance of advocacy in convincing policy makers of the feasibility and benefit of NBS for sickle cell disease when coupled to early care.
The Development of a Surface Finisher of Car Park Slab Using Waterborne Silicon Acrylic with Polyamide [Part II: Safety Tests]
Authors: Gong Junho , Jeong Hoseong , Cho Dooyong
Published: January 2019

Due to the environmental concerns of solventborne coating systems, environmental directives have recently been promulgated in many countries. Additionally, integrated environmental policies have been pushed in many fields to minimise influences on the environment. Waterborne silicon acrylic finishers have gained much interest to replace the traditional finishing system. To satisfy the requirements, a waterborne finisher with polyamide was previously developed and its performance was determined. For further safety assessment, various tests were conducted, such as gas toxicity, heavy metals tests, chemical resistance test and chloride migration test, followed by equivalent standards. In the cases of gas toxicity and heavy metals evaluations, both results were acceptable considering their corresponding standards, e.g. KS F 2271, KS F 3888-2 and BS EN 71-3. Based on the evaluation, silicon acrylic with 30% mix ratio of polyamide resin (SA+PR30%) could be implemented as an environmentally friendly finisher for various applications. In the chemical resistance and chloride migration test results, the developed finisher showed a barrier effect in the chemical environment. Thus, the developed finisher could be an alternative finisher applicable for slabs in chemical industrial areas.
Low Temperature Solution-Processable 3D-Patterned Charge Recombination Layer for Organic Tandem Solar Cells
Authors: Choi Jin Woo , Jin Jong Woo , Tondelier Denis , Bonnassieux Yvan , Geffroy Bernard
Published: January 2019

We propose a novel method to pattern the charge recombination layer (CRL) with a low-temperature solution-processable ZnO layer (under 150 °C) for organic solar cell applications. Due to the optimal drying process and thermal annealing condition, ZnO sol-gel particles formed a three-Dimensional (3D) structure without using a high temperature or ramping method. The generated 3D nano-ripple pattern showed a height of around 120 nm, and a valley-to-valley distance of about 500 nm. Based on this newly developed ZnO nano-ripple patterning technique, it was possible to pattern the CRL without damaging the underneath layers in tandem structure. The use of nano-ripple patterned ZnO as the part of CRL, led to the concomitant improvement of the power conversion efficiency (PCE) of about 30%, compared with non-patterned CRL device.
Augmentation of DMLS Biomimetic Dental Implants with Weight-Bearing Strut to Balance of Biologic and Mechanical Demands: From Bench to Animal
Authors: Chang Jenny Zwei-Chieng , Tsai Pei-I , Kuo Mark Yen-Ping , Sun Jui-Sheng , Chen San-Yuan , Shen Hsin-Hsin
Published: January 2019

A mismatch of elastic modulus values could result in undesirable bone resorption around the dental implant. The objective of this study was to optimize direct metal laser sintering (DMLS)-manufactured Ti6Al4V dental implants’ design, minimize elastic mismatch, allow for maximal bone ingrowth, and improve long-term fixation of the implant. In this study, DMLS dental implants with different morphological characteristics were fabricated. Three-point bending, torsional, and stability tests were performed to compare the mechanical properties of different designs. Improvement of the weaker design was attempted by augmentation with a longitudinal 3D-printed strut. The osseointegrative properties were evaluated. The results showed that the increase in porosity decreased the mechanical properties, while augmentation with a longitudinal weight-bearing strut can improve mechanical strength. Maximal alkaline phosphatase gene expression of MG63 cells attained on 60% porosity Ti6Al4V discs. In vivo experiments showed good incorporation of bone into the porous scaffolds of the DMLS dental implant, resulting in a higher pull-out strength. In summary, we introduced a new design concept by augmenting the implant with a longitudinal weight-bearing strut to achieve the ideal combination of high strength and low elastic modulus; our results showed that there is a chance to reach the balance of both biologic and mechanical demands.
The Development of a Surface Finisher of Car Park Slab Using Waterborne Silicon Acrylic with Polyamide [Part I: Performance Evaluation]
Authors: Jeong Hoseong , Gong Junho , Yoon Wanseok , Cho Dooyong
Published: January 2019

A waterborne coating system for car park slab has recently gained interest as an alternative for solvent-based finishing materials due to environmental concerns and prolongation of service life. However, water-based finishers, regardless of their eco-friendly properties, have relatively lower hardness compared to traditional finishing systems. In order to overcome this obstacle, a hybrid technology was used to develop a substitute surface finisher for car park slab and its performance was evaluated according to the KS (Korean Standard) F 4937. Initially, the proper mix ratio of polyamide was found by comparing adhesion via pull-off-test results and other performance evaluation tests. From the test results, it was found that mixing polyamide with silicon acrylic finisher caused an increase in adhesion strength. Silicon acrylic with a 30% mix ratio of polyamide resin (SA+PR30%) was selected to perform the rest of the tests and the results satisfied the acceptance criteria of KS F 4937 and were compared with a recent water-based polyurethane finisher with cementitious powder (WPC). Finally, it was verified that the developed finisher could be an alternative finisher of urethane and epoxy finishers as it has good mechanical properties and emit less volatile organic compounds (VOC).
Effects on the Mechanical Properties of Nacre-Like Bio-Hybrid Membranes with Inter-Penetrating Petal Structure Based on Magadiite
Authors: Ge Mingliang , Wang Xubin , Du Mingyi , Liang Guodong , Hu Guoqing , S.M. Jahangir Alam
Published: January 2019

Rigid biological systems are increasingly becoming a source of inspiration for the fabrication of the advanced functional materials due to their diverse hierarchical structures and remarkable engineering properties. As a bionic biomaterial with a clear layered structure, excellent mechanical properties, and interesting rainbow colors, nacre has become one of the most attractive models for novel artificial materials design. In this research paper, the tough and strong nacre-like bio-hybrid membranes with an interpenetrating petals structure were fabricated from chitosan (CS) and magadiite (MAG) clay nanosheets through the gel-casting self-assembling method. The analyses from X-ray diffraction (XRD), scanning electron microscope (SEM), and observations of water droplets on membranes indicated that the nacre-like hybrid membranes had a layered compact structure. Fourier transforms infrared spectroscopy (FTIR) analyses suggested that the CS molecular chains formed chemical bonds and hydrogen bonds with MAG layers. The inter-penetrating petal layered structure had a good effect on the mechanical properties of a nacre-like bio-hybrid membranes and the tensile strength of the hybrid membranes could reach at 78.6 MPa. However, the transmission analyses of the results showed that the hybrid membranes still had a certain visible light transmittance. Finally, the hybrid membranes possessed an intriguing efficient fire-shielding property during exposure to the flame of alcohol burner. Consequently, the great biocompatibility and excellent mechanical properties of the bio-hybrid membranes with the special interpenetrating petals structure provides a great opportunity for these composites to be widely applied in biomaterial research.