bluuebunny/crossref_metadata_2025 huggingface dataset:
Robust Input Shaping Commands with First-Order Actuators
Authors: Sung Yoon-Gyung , Lee Seongjun
Published: August 2024

This paper presents robust input shaping commands with first-order actuators utilizing a classical robust input shaper for practical applications in input shaping technology. An ideal input shaping command can deviate due to actuator dynamics so that the modified command has a detrimental effect on the performance of oscillation reduction in feedforward control applications. A zero-vibration-derivative (ZVDF) shaper with first-order actuators is analytically proposed using a phasor–vector approach, an exponential function for the approximation of the dynamic response of first-order actuators and the usage of the ZVD shaper. In addition, an equivalent transformation is utilized based on the superposition principle for the convenient inclusion of first-order actuator dynamics and is applied to the individual segment input command. The residual deflection and robustness of the proposed robust input shaping commands are numerically evaluated and compared with those of a conventional ZVD shaper with respect to the parameter uncertainties of flexible systems and actuators. The robust input shaping commands that are possible with first-order actuators are experimentally validated, presenting a better robustness and residual deflection reduction performance than the classical ZVD shaper on a mini bridge crane.
Residual Stress and Warping Analysis of the Nano-Silver Pressureless Sintering Process in SiC Power Device Packaging
Authors: Tian Wenchao , Li Dexin , Dang Haojie , Liang Shiqian , Zhang Yizheng , Zhang Xiaojun , Chen Si , Yu Xiaochuan
Published: August 2024

Chip bonding, an essential process in power semiconductor device packaging, commonly includes welding and nano-silver sintering. Currently, most of the research on chip bonding technology focuses on the thermal stress analysis of tin–lead solder and nano-silver pressure-assisted sintering, whereas research on the thermal stress analysis of the nano-silver pressureless sintering process is more limited. In this study, the pressureless sintering process of nano-silver was studied using finite element software, with nano-silver as an interconnect material. Using the control variable method, we analyzed the influences of sintering temperature, cooling rate, solder paste thickness, and solder paste area on the residual stress and warping deformation of power devices. In addition, orthogonal experiments were designed to optimize the parameters and determine the optimal combination of the process parameters. The results showed that the maximum residual stress of the module appeared on the connection surface between the power chip and the nano-silver solder paste layer. The module warping deformation was convex warping. The residual stress of the solder layer increased with the increase in sintering temperature and cooling rate. It decreased with the increase in coating thickness. With the increase in the coating area, it showed a wave change. Each parameter influenced the stress of the solder layer in this descending order: sintering temperature, cooling rate, solder paste area, and solder paste thickness. The residual stress of the nano-silver layer was 24.83 MPa under the optimal combination of the process parameters and was reduced by 29.38% compared with the original value of 35.162 MPa.
Towards Cell-Permeable Hepatitis B Virus Core Protein Variants as Potential Antiviral Agents
Authors: Bendahmane Sanaa , Follo Marie , Zhang Fuming , Linhardt Robert J.
Published: August 2024

Hepatitis B virus (HBV) infection remains a major health threat with limited treatment options. One of various new antiviral strategies is based on a fusion of Staphylococcus aureus nuclease (SN) with the capsid-forming HBV core protein (HBc), termed coreSN. Through co-assembly with wild-type HBc-subunits, the fusion protein is incorporated into HBV nucleocapsids, targeting the nuclease to the encapsidated viral genome. However, coreSN expression was based on transfection of a plasmid vector. Here, we explored whether introducing protein transduction domains (PTDs) into a fluorescent coreSN model could confer cell-penetrating properties for direct protein delivery into cells. Four PTDs were inserted into two different positions of the HBc sequence, comprising the amphiphilic translocation motif (TLM) derived from the HBV surface protein PreS2 domain and three basic PTDs derived from the Tat protein of human immunodeficiency virus-1 (HIV-1), namely Tat4, NP, and NS. To directly monitor the interaction with cells, the SN in coreSN was replaced with the green fluorescent protein (GFP). The fusion proteins were expressed in E. coli, and binding to and potential uptake by human cells was examined through flow cytometry and fluorescence microscopy. The data indicate PTD-dependent interactions with the cells, with evidence of uptake in particular for the basic PTDs. Uptake was enhanced by a triplicated Simian virus 40 (SV40) large T antigen nuclear localization signal (NLS). Interestingly, the basic C terminal domain of the HBV core protein was found to function as a novel PTD. Hence, further developing cell-permeable viral capsid protein fusions appears worthwhile.
Preliminary Study of the Characterization of the Viable but Noncultivable State of Yersinia enterocolitica Induced by Chloride and UV Irradiation
Authors: Hu Xueyu , Wang Xiaoxu , Ren Honglin , Li Chengwei , Zhang Bo , Shi Ruoran , Wang Yuzhu , Lu Shiying , Li Yansong , Lu Qiang , Liu Zengshan , Hu Pan
Published: August 2024

The viable but non-culturable (VBNC) state is a survival strategy for many foodborne pathogens under adverse conditions. Yersinia enterocolitica (Y. enterocolitica) as a kind of primary foodborne pathogen, and it is crucial to investigate its survival strategies and potential risks in the food chain. In this study, the effectiveness of ultraviolet (UV) irradiation and chlorine treatment in disinfecting the foodborne pathogen Y. enterocolitica was investigated. The results indicated that both UV irradiation and chlorine treatment can induce the VBNC state in Y. enterocolitica. The bacteria completely lost culturability after being treated with 25 mg/L of NaClO for 30 min and a UV dose of 100 mJ/cm². The number of culturable and viable cells were detected using plate counting and a combination of fluorescein and propidium iodide (live/dead cells). Further research found that these VBNC cells exhibited reduced intracellular Adenosine Triphosphate (ATP) levels, and increased levels of reactive oxygen species (ROS) compared to non-induced cells. Morphologically, the cells changed from a rod shape to a shorter, coccobacillary shape with small vacuoles forming at the edges, indicating structural changes. Both condition-induced VBNC-state cells were able to resuscitate in tryptic soy broth (TSB) medium supplemented with Tween 80, sodium pyruvate, and glucose. These findings contribute to a better understanding of the survival mechanisms of Y. enterocolitica in the environment and are of significant importance for the development of effective disinfection strategies.
Shifts in Structure and Assembly Processes of Root Endophytic Community Caused by Climate Warming and Precipitation Increase in Alpine Grassland
Authors: Wei Xiaoting , Han Bing , Zhang Jinxin , Shao Xinqing
Published: August 2024

Climate change poses great challenges to the survival of plants. Plant endophytes play important roles in improving plant adaptability. However, our knowledge of the effects of climate change on endophytic community structures is limited. Relying on a field experimental platform simulating climate warming, precipitation increases, and their combination in an alpine grassland, the root endophytic bacterial community structures and assembly processes of three coexisting plant species (Elymus nutans, Kobresia humilis, and Melissilus ruthenicus) were measured. The results indicated that Proteobacteria was the dominant phylum, with a relative abundance ranging from 50% to 80%, followed by Actinobacteria and Bacteroidetes. Bacterial diversity decreased significantly under the combined treatment for all three plant species, with the largest reduction observed in E. nutans. The climate manipulation treatments had a minimal effect on the endophytic bacterial community structures. The relative abundance of Burkholderiaceae increased significantly under the combined treatment for the three plant species. Moreover, the endophytic community assembly processes changed from stochastic dominated under control plots to deterministic dominated under the combined plots for E. nutans, while this shift was reversed for M. ruthenicus. The root endophytic bacterial community was affected by the soil’s available nitrogen and stoichiometric ratio. These results revealed that the sensitivity of endophyte community structures to climate change varies with host plant species, which has implications for plant fitness differences.
A Novel C-Terminal Truncated Bacteriocin Found by Comparison between Leuconostoc mesenteroides 406 and 213M0 Isolated from Mongolian Traditional Fermented Milk, Airag
Authors: Hasiqimuge  , Hano Chihiro , Arakawa Kensuke , Yoshida Saki , Zhao Junliang , Toh Hidehiro , Morita Hidetoshi , Miyamoto Taku
Published: August 2024

Bacteriocins produced by lactic acid bacteria are known to be useful tools for food biopreservation and fermentation control. Leuconostoc mesenteroides subsp. mesenteroides 406 and 213M0 isolated from different samples of Mongolian traditional fermented milk, airag, had been reported to produce listericidal bacteriocin-like inhibitory substances with similar but slightly different properties. In this study, the antibacterial properties and the related gene sequences of both strains were compared, and then their bacteriocins were purified and identified. Strain 406 was superior to strain 213M0 in cell growth and antibacterial activity against many strains. However, the activity of 213M0 was stronger than that of 406 against a few strains. DNA sequencing revealed two and three plasmids in 406 and 213M0, respectively, and each one of them harbored an almost identical mesentericin Y105–B105 gene cluster. Removal of these plasmids resulted in a complete loss of activity, indicating that the antibacterial activity of both strains was generated by bacteriocins encoded on the plasmids. Mesentericins Y105 and B105 were purified from both cultures, and another novel bacteriocin, named mesentericin M, was identified from the 213M0 culture only. Its structural gene was coded on a 213M0 plasmid and, surprisingly, its C-terminal three amino acid residues were post-translationally cleaved. To our knowledge, this is the first report of a C-terminal truncated bacteriocin. In conclusion, the novel bacteriocin should be mainly responsible for the difference in antibacterial properties between the two strains.
Clostridioides difficile Infections in Children: What Is the Optimal Laboratory Diagnostic Method?
Authors: Suleiman Mohammed , Tang Patrick , Imam Omar , Morales Princess , Altrmanini Diyna , Roberts Jill C. , Pérez-López Andrés
Published: August 2024

The diagnosis of Clostridioides difficile infection (CDI) in the pediatric population is complicated by the high prevalence of asymptomatic colonization, particularly in infants. Many laboratory diagnostic methods are available, but there continues to be controversy over the optimal laboratory testing approach to diagnose CDI in children. We evaluated commonly used C. difficile diagnostic commercial tests in our pediatric hospital population at Sidra Medicine in Doha, Qatar. Between June and December 2023, 374 consecutive stool samples from pediatric patients aged 0–18 years old were tested using: Techlab C. diff Quik Chek Complete, Cepheid GeneXpert C. difficile, QIAstat-Dx Gastrointestinal Panel, and culture using CHROMagar C. difficile. The results of these tests as standalone methods or in four different testing algorithms were compared to a composite reference method on the basis of turnaround time, ease of use, cost, and performance characteristics including specificity, sensitivity, negative predictive value, and positive predictive value. Our study showed variability in test performance of the different available assays in diagnosing CDI. In our population, a testing algorithm starting with Cepheid GeneXpert C. difficile PCR assay or QIAstat-Dx Gastrointestinal panel as a screening test followed by toxin immunoassay for positive samples using the Techlab C. diff Quik Chek Complete kit showed the best performance (100% specificity and 100% positive predictive value) when combined with clinical review of the patient to assess risk factors for CDI, clinical presentation, and alternative causes of diarrhea.
Graphitic Carbon Nitride Catalyzes the Reduction of the Azo Bond by Hydrazine under Visible Light
Authors: Okolie Makobi C. , Ollordaa Glory G. , Ramidi Gopal R. , Yan Xin , Quan Yufeng , Wang Qingsheng , Li Yingchun
Published: August 2024

Graphitic carbon nitride is a semiconducting material of a graphite-like 2D layered structure. It is well known for its photocatalytic properties, which can be exploited for solar-light-driven water splitting and degradation of organic pollutants. Here, we report its capabilities of catalyzing the reduction of the azo bond by hydrazine to two amines under visible light. This photocatalytic reaction provides a novel, appealing way to reduce azo dye wastes as pollutants other than degradation. With this method, the azo dye wastes can be photochemically converted to amines, which can be used as precursors for new azo dyes.
Non-Specific Elevated Serum Free Fatty Acids in Lung Cancer Patients: Nutritional or Pathological?
Authors: Shao Yelin , Wang Sicong , Xu Xiaohang , Sun Ce , Cai Fei , Guo Qian , Wu Ming , Yang Min , Wu Xifeng
Published: August 2024

Importance: The reprogramming of lipid metabolism is a significant feature of tumors, yet the circulating levels of fatty acids in lung cancer patients remain to be explored. Moreover, the association between fatty acid levels and related factors, including nutritional intake, tumor metabolism, and tumor immunity, has been rarely discussed. Objectives: To explore the differences in serum free fatty acids between lung cancer patients and healthy controls, and investigate the factors associated with this phenomenon. Design and participants: A case-control study enrolled 430 primary lung cancer patients and 430 healthy controls. The whole population had a medium [Q1, Q3] age of 48.0 [37.0, 58.9] years, with females comprising 56% of the participants. The absolute quantification of 27 serum free fatty acids (FFAs) was measured using a liquid chromatography–mass spectrometry (LC-MS/MS) detection. Data, including dietary intake, blood indicators, and gene expression of lung tissues, were obtained from questionnaires, blood tests, and RNA-sequencing. Statistical differences in FFA levels between lung cancer patients and healthy controls were investigated, and related contributing factors were explored. Results: Levels of 22 FFAs were significantly higher in lung cancer patients compared to those in healthy controls, with fold changes ranging from 1.14 to 1.69. Lung cancer diagnosis models built with clinical and FFA features yielded an area under the receiver operating characteristic curve (AUROC) of 0.830 (0.780–0.880). Total fatty acids (TFAs), monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs) showed no significant dietary–serum associations, indicating that the elevations might not be attributed to an excessive intake of relevant fatty acids from the diet. For RNA-sequencing of lung tissues, among the 68 lipid metabolism genes, 26 genes showed significant upregulation (FDR < 0.05), while 33 genes exhibited significant downregulation, indicating the involvement of the fatty acids in the tumor metabolism. Through joint analysis with immune cells and inflammatory factors in the blood, fatty acids might exert suppressing effects on tumor immunity. Conclusions: Lung cancer patients had elevated levels of serum free fatty acids compared to healthy individuals. The elevations might not be attributed to an excessive intake of relevant fatty acids from the diet but related to pathological factors of tumor metabolism and immunity. These findings will complement research on fatty acid metabolism of lung cancer and provide insights into potential intervention targets.
IA-Body Composition CT at T12 in Idiopathic Pulmonary Fibrosis: Diagnosing Sarcopenia and Correlating with Other Morphofunctional Assessment Techniques
Authors: Fernández-Jiménez Rocío , Sanmartín-Sánchez Alicia , Cabrera-César Eva , Espíldora-Hernández Francisco , Vegas-Aguilar Isabel , Amaya-Campos María del Mar , Palmas-Candia Fiorella Ximena , Claro-Brandner María , Olivares-Alcolea Josefina , Simón-Frapolli Víctor José , Cornejo-Pareja Isabel , Guirado-Peláez Patricia , Vidal-Suárez Álvaro , Sánchez-García Ana , Murri Mora , Garrido-Sánchez Lourdes , Tinahones Francisco J. , Velasco-Garrido Jose Luis , García-Almeida Jose Manuel
Published: August 2024

Background: Body composition (BC) techniques, including bioelectrical impedance analysis (BIVA), nutritional ultrasound® (NU), and computed tomography (CT), can detect nutritional diagnoses such as sarcopenia (Sc). Sc in idiopathic pulmonary fibrosis (IPF) is associated with greater severity and lower survival. Our aim was to explore the correlation of BIVA, NU and functional parameters with BC at T12 level CT scans in patients with IPF but also its relationship with degree of Sc, malnutrition and mortality. Methods: This bicentric cross-sectional study included 60 IPF patients (85.2% male, 70.9 ± 7.8 years). Morphofunctional assessment (MFA) techniques included BIVA, NU, CT at T12 level (T12-CT), handgrip strength, and timed up and go. CT data were obtained using FocusedON®. Statistical analysis was conducted using JAMOVI version 2.3.22 to determine the cutoff points for Sc in T12-CT and to analyze correlations with other MFA techniques. Results: the cutoff for muscle area in T12-CT was ≤77.44 cm2 (area under the curve (AUC) = 0.734, sensitivity = 41.7%, specificity = 100%). The skeletal muscle index (SMI_T12CT) cutoff was ≤24.5 cm2/m2 (AUC = 0.689, sensitivity = 66.7%, specificity = 66.7%). Low SMI_T12CT exhibited significantly reduced median survival and higher risk of mortality compared to those with normal muscle mass (SMI cut off ≥ 28.8 cm/m2). SMI_T12CT was highly correlated with body cell mass from BIVA (r = 0.681) and rectus femoris cross-sectional area (RF-CSA) from NU (r = 0.599). Cronbach’s α for muscle parameters across different MFA techniques and CT was 0.735, confirming their validity for evaluating muscle composition. Conclusions: T12-CT scan is a reliable technique for measuring low muscle mass in patients with IPF, specifically when the L3 vertebrae are not captured. An SMI value of <28.8 is a good predictor of low lean mass and 12-month mortality in IPF patients.
PCR Detection of Bartonella spp. and Borreliella spp. DNA in Dry Blood Spot Samples from Human Patients
Authors: Clark Kerry L. , Hartman Shirley
Published: August 2024

Lyme disease is the most commonly reported vector-borne disease in the United States. Bartonella constitute an additional zoonotic pathogen whose public health impact and diversity continue to emerge. Rapid, sensitive, and specific detection of these and other vector-borne pathogens remains challenging, especially for patients with persistent infections. This report describes an approach for DNA extraction and PCR testing for the detection of Bartonella spp. and Borreliella spp. from dry blood spot (DBS) specimens from human patients. The present study included extraction of DNA and PCR testing of DBS samples from 105 patients with poorly defined, chronic symptoms labeled as Lyme-Like Syndromic Illness (LLSI). Bartonella spp. DNA was detected in 20/105 (19%) and Borreliella spp. DNA was detected in 41/105 (39%) patients with LLSI. Neither group of organisms was detected in DBS samples from 42 healthy control subjects. Bartonella spp. 16S–23S rRNA internal transcribed spacer sequences were highly similar to ones previously identified in yellow flies, lone star ticks, a human patient from Florida, mosquitoes in Europe, or B. apihabitans and choladocola strains from honeybees. These human strains may represent new genetic strains or groups of human pathogenic species of Bartonella. The 41 Borreliella spp. flaB gene sequences obtained from human patients suggested the presence of four different species, including B. burgdorferi, B. americana, B. andersonii, and B. bissettiae/carolinensis-like strains. These results suggest that specific aspects of the DBS DNA extraction and PCR approach enabled the detection of Bartonella spp. and Borreliella spp. DNA from very small amounts of human whole blood from some patients, including specimens stored on filter paper for 17 years.
Unlocking the Potential: Epstein-Barr Virus (EBV) in Gastric Cancer and Future Treatment Prospects, a Literature Review
Authors: Corallo Salvatore , Lasagna Angioletta , Filippi Beatrice , Alaimo Domiziana , Tortorella Anna , Serra Francesco , Vanoli Alessandro , Pedrazzoli Paolo
Published: August 2024

Gastric cancer (GC) is a complex disease with various etiologies. While Helicobacter pylori infection is still one of the leading risk factors for GC, increasing evidence suggests a link between GC and other infective agents such as Epstein Bar Virus (EBV). EBV-associated gastric cancer (EBVaGC) is now recognized as a distinct subgroup of GC, and the complex interactions between the virus and gastric mucosa may influence its development. A recent integrative analysis of the genome and proteome of GC tissues by The Cancer Genome Atlas project has identified EBVaGC as a specific subtype characterized by PIK3CA and ARID1A mutations, extensive DNA hyper-methylation, and activation of immune signaling pathways. These molecular characteristics are markers of the unique molecular profile of this subset of GC and are potential targets for therapy. This review aims to provide an overview of the current knowledge on EBVaGC. It will focus on the epidemiology, clinic-pathological features, and genetic characteristics of EBVaGC. Additionally, it will discuss recent data indicating the potential use of EBV infection as a predictive biomarker of response to chemotherapy and immune checkpoint inhibitors. The review also delves into potential therapeutic approaches for EBVaGC, including targeted therapies and adoptive immunotherapy, highlighting the promising potential of EBV as a therapeutic target.
Effective Supply Chain Strategies in Addressing Demand and Supply Uncertainty: A Case Study of Ethiopian Pharmaceutical Supply Services
Authors: Bilal Arebu Issa , Bititci Umit Sezer , Fenta Teferi Gedif
Published: August 2024

Background: Ensuring the consistent availability of essential medicines is crucial for effective healthcare systems. However, Ethiopian public health facilities have faced frequent stockouts of crucial medications, highlighting systemic challenges such as inadequate forecasting, prolonged procurement processes, a disjointed distribution system, suboptimal data quality, and a shortage of trained professionals. This study focuses on the Ethiopian Pharmaceutical Supply Services (EPSS), known for its highly unstable and volatile supply chain, aiming to identify risks and mitigation strategies. Methods: Using a mixed-method approach involving surveys and interviews, the research investigates successful and less successful strategies, key success factors, and barriers related to pharmaceutical shortages. Results: Proactive measures such as communication, stock assessment, supervision, and streamlined procurement are emphasized as vital in mitigating disruptions, while reactive strategies like safety stock may lack long-term efficacy. The study highlights the importance of aligning supply chain strategies with product uncertainties, fostering collaboration, and employing flexible designs for resilience. Managerial implications stress the need for responsive structures that integrate data quality, technology, and visibility. Conclusions: This study contributes by exploring proactive and reactive strategies, elucidating key success factors for overcoming shortages in countries with unstable supply chains, and offering actionable steps for enhancing supply chain resilience. Embracing uncertainty and implementing proactive measures can help navigate volatile environments, thereby enhancing competitiveness and sustainability.
Longitudinal Wave Defect Detection Technology Based on Ablation Mechanism
Authors: Wang Jian , Guo Xing , Xun Yanqin , Liu Junzuo , Guo Xin , Li Ya
Published: August 2024

For laser ultrasound in the thermoelastic mechanism, excitation of ultrasonic body wave signal is weak, it is not easy to realize the detection of deep defects inside the workpiece. While the ablation mechanism produces a high and practical ultrasonic signal-to-noise ratio, this paper is based on the generation mechanism of laser ablation excitation of ultrasonic waves, the establishment of laser ultrasound in the ablation mechanism in the aluminum plate excitation and propagation of ultrasonic numerical model, through the solution, obtained the ultrasonic acoustic field map, discussed the ablation mechanism of the laser ultrasonic body wave acoustic field directionality. Additionally, the preliminary verification of the validity of the model is presented. Then, in order to explore the application potential of high signal-to-noise ratio longitudinal waves in defect detection, defects of different depths are preset in the model for simulation calculations, and waveform and acoustic field analyses are performed on the simulation results to study the ultrasonic propagation paths inside the member and the interaction with the defects, and the transverse position and depth of the internal defects are judged by using B-scan imaging. Finally, experimental validation is carried out. The experimental results are highly consistent with the simulation model, and the defect experiments can qualitatively determine the location of internal defects and verify the practicality and accuracy of the model.
Construction of Optimal Regeneration System for Chrysanthemum ‘11-C-2’ Stem Segment with Buds
Authors: Chen Qingbing , Gao Kang , Pan Bo , Wang Yaoyao , Chen Lijie , Yu Junjun , Wang Lili , Fan Yongming , Li Haiying , Huang Conglin
Published: August 2024

Chrysanthemum morifolium ‘11-C-2’ is a variety of chrysanthemums with high ornamental and tea value, experiencing significant market demand. However, as cultivation areas expand, issues such as viral infection, germplasm degradation, low proliferation coefficient, and slow proliferation rate arise, necessitating the establishment of an efficient in vitro regeneration system. This study, based on the principles of orthogonal experimental design, explored the regeneration system of Chrysanthemum cultivar ‘11-C-2’ using sterile seedlings. The research focused on three key stages: adventitious bud differentiation, rooting culture, and acclimatization–transplantation, employing shoot-bearing stem segments and leaves as explants. The findings indicate that the optimal explant for the Chrysanthemum ‘11-C-2’ sterile seedlings is the shoot-bearing stem segment. The best medium for adventitious bud differentiation was determined to be MS supplemented with 1.5 mg/L 6-BA and 0.5 mg/L NAA. Bud differentiation began on day 17 with a 100% differentiation rate, completing around day 48. The maximum differentiation coefficient reached 87, with an average of 26.67. The adventitious buds were then cultured for rooting in the optimal medium of 1/2 MS supplemented with 0.1 mg/L NAA. Rooting was initiated on day 4 and was completed by day 14, achieving a rooting rate of 97.62%. After a 5-day acclimatization under natural light, the rooted seedlings were transplanted into a growth substrate with a peat-to-vermiculite ratio of 1:2. The plants exhibited optimal growth, with a transplantation survival rate of 100%. The findings provide data support for the efficient large-scale propagation of ‘11-C-2’ and lay the foundation for germplasm preservation and genetic transformation research of tea chrysanthemums.
Influence of Functional Traits of Dominant Species of Different Life Forms and Plant Communities on Ecological Stoichiometric Traits in Karst Landscapes
Authors: Wang Yang , Zhang Limin , Feng Ling , Fan Zuhong , Deng Ying , Feng Tu
Published: August 2024

Assessing the functional traits and ecological stoichiometric characteristics of dominant species across different life forms within plant communities in karst environments and investigating the inherent connection between them can provide insights into how species adjust their functional attributes in response to habitat heterogeneity. This approach offers a more comprehensive understanding of ecosystem processes and functions in contrast to examination of the taxonomic diversity of species. This study examines the relationship between the functional characteristics of dominant species in plant communities of various life forms in karst environments, focusing on deciduous leaf–soil ecological stoichiometry. The investigation relies on community science surveys, as well as the determination and calculation of plant functional traits and ecological stoichiometries, in plant communities of various life forms in Guizhou (a province of China). The findings of our study revealed considerable variability in the functional trait characteristics of dominant species across different plant-community life forms. Specifically, strong positive correlations were observed among plant height (PLH), leaf area (LA), leaf dry matter content (LDMC), and specific leaf area (SLA) in the dominant species. Additionally, our results indicated no significant differences in leaf ecological stoichiometry among different life forms. However, we did observe significant differences and strong positive correlations between soil N:P, withered material C:N, and apomictic C:P. Furthermore, our study found that plant height (PLH), leaf area (LA), and specific leaf area (SLA) were particularly sensitive to the ecological stoichiometry of soil and apomixis. The results of our study suggest that the functional traits of diverse plant-community life forms in karst regions are capable of adapting to environmental changes through various expressions and survival strategies. The development of various plant-community life forms in karst areas is particularly vulnerable to phosphorus limitation, and the potential for litter decomposition and soil nutrient mineralization is comparatively weaker. The functional traits of various plant-community life forms in karst regions exhibit greater sensitivity to both the soil’s C:N ratio and the C:N ratio of apomictic material. Habitat variations may influence the ecological stoichiometric characteristics of the plant leaf–apomictic soil continuum.
Polymer Matrix Nanocomposites Fabricated with Copper Nanoparticles and Photopolymer Resin via Vat Photopolymerization Additive Manufacturing
Authors: Gil Leon D. , Monteiro Sergio Neves , Colorado Henry A.
Published: August 2024

This investigation explores the fabrication of polymer matrix nanocomposites via additive manufacturing (AM), using a UV photopolymerization resin and copper nanoparticles (Cu-NPs) with vat photopolymerization 3D printing technology. The aim in this study is to investigate the mentioned materials in different formulations in terms of inexpensive processing, the property related variability, and targeting multifunctional applications. After the AM process, samples were post-cured with UV light in order to obtain better mechanical properties. The particles and resin were mixed using an ultrasonicator, and the particle contents used were 0.0, 0.5, and 1.0 wt %. The process used in this investigation was simple and inexpensive, as the technologies used are quite accessible, from the 3D printer to the UV curing device. These formulations were characterized with scanning electron microscopy (SEM) to observe the materials’ microstructure and tensile tests to quantify stress–strain derived properties. Results showed that, besides the simplicity of the process, the mixing was effective, which was observed in the scanning electron microscope. Additionally, the tensile strength was increased with the UV irradiation exposure, while the strain properties did not change significantly.
On-Substrate Preparation of a Poly(triphenylamino azomethine) for Electrochromic Devices
Authors: Filiatrault Heather L. , Muras Kacper , Wałęsa-Chorab Monika , Skene W. G.
Published: August 2024

An electroactive polyazomethine was prepared directly on a transparent electrode by the polycondensation of bis(triphenylamine) dialdehyde and its complementary methoxytriphenylamine diamine. The spray-and-bake method of coating the electrode for preparing electrochromic layers could be upscaled to prepare working devices larger than standard test devices. The film prepared by thermally annealing the complementary monomers was both electroactive and switched its color with an applied potential. The yellow electrochromic polyazomethine could be electrochemically oxidized reversibly to obtain a blue film. The electrochromic test device fabricated from the polyazomethine was operated upwards of 1 h for performance assessment. The electrochromic response times of the electrochromic device were ca. 3.3 and 1.2 s for the coloration and bleaching, respectively. The upscaled device prepared by the straightforward coating approach had consistent metrics with the small-area test device.
Recent Advances in Fire-Retardant Silicone Rubber Composites
Authors: Tang Yi-Hao , Liu Jun , Chen Zuan-Yu , Li Yang , Cao Cheng-Fei , Zhang Guo-Dong , Tang Long-Cheng
Published: August 2024

Silicone rubber (SR), as one kind of highly valuable rubber material, has been widely used in many fields, e.g., construction, transportation, the electronics industry, automobiles, aviation, and biology, owing to its attractive properties, including high- and low-temperature resistance, weathering resistance, chemical stability, and electrical isolation, as well as transparency. Unfortunately, the inherent flammability of SR largely restricts its practical application in many fields that have high standard requirements for flame retardancy. Throughout the last decade, a series of flame-retardant strategies have been adopted which enhance the flame retardancy of SR and even enhance its other key properties, such as mechanical properties and thermal stability. This comprehensive review systematically reviewed the recent research advances in flame-retarded SR materials and summarized and introduced the up-to-date design of different types of flame retardants and their effects on flame-retardant properties and other performances of SR. In addition, the related flame-retardant mechanisms of the as-prepared flame-retardant SR materials are analyzed and presented. Moreover, key challenges associated with these various types of FRs are discussed, and future development directions are also proposed.
Advancements in Methane Dry Reforming: Investigating Nickel–Zeolite Catalysts Enhanced by Promoter Integration
Authors: Fakeeha Anis H. , Ibrahim Ahmed A. , Osman Ahmed I. , Abasaeed Ahmed E. , Alanazi Yousef M. , Almubaddel Fahad S. , Al-Fatesh Ahmed S.
Published: August 2024

A promising method for converting greenhouse gases such as CO2 and CH4 into useful syngas is the dry reformation of methane (DRM). 5Ni-ZSM-5 and 2 wt.% Ce, Cs, Sr, Fe, and Cu-promoted 5Ni-ZSM-5 catalysts are investigated for the DRM at 700 °C under atmospheric pressure. The characterization, including XRD, TPR, TPD, TPO, N2 adsorption–desorption, TGA, TEM, and Raman spectroscopy, revealed that the catalyst’s active sites are distributed throughout the pore channels and on the surface, contributing to the stability of the catalyst. Specifically, the CO2-TPO followed by the O2-TPO experiment using spent catalysts confirmed the oxidizing capacity of CO2 during the DRM reaction. The Ce-promoted catalyst showed the greatest increase in catalytic activity among other catalysts. The 5Ni+2Ce-ZSM-5 catalyst exhibited twice the concentration of acid sites compared to the Cs-promoted counterpart, even though both catalysts achieved similar quantities of active and basic sites. Without compromising H2 and CO selectivity, this finding underscores the crucial role of acid sites in enhancing CH4 and CO2 conversion. With a GHSV of 42,000 mL/(h.gcat), the 5Ni+2Ce-ZSM-5 catalyst demonstrated impressive CH4 conversion rates of 42% at 700 °C and 70% at 800 °C. The reactants spend more time over catalysts during the subsequent reduction of GHSV to 21,000 mL/(h.gcat), resulting in the best catalytic performance with 80% CH4 and 83% CO2 conversions.
Sustainable Utilization of Novosadska variety Buckwheat as Cultivated Biodiversity-Friendly Crop
Authors: Pisinov Boris , Rakić Radojica , Rakić Sveto , Sekulić Zoran Ž. , Milićević Tijana , Kulić Gordana , Đurović Sanja
Published: August 2024

Buckwheat is important not only for its role in enhancing soil quality and preventing erosion but also for its excellent nutritional profile, making it suitable for use in functional foods. This study aimed to investigate how long-term storage (3, 6, and 9 months) affects chemical, nutritional, and antioxidative properties, phenolic acids, and the bioflavonoid profiles of Novosadska variety buckwheat. Standard methods were used for quality determinations, and instrumental methods (spectrophotometry, reverse-phase high-performance liquid chromatography) were employed to determine antioxidant activity and bioactive compounds in Novosadska variety buckwheat. One-way ANOVA and Tukey’s HSD post hoc tests were performed for statistical data processing. Throughout the storage period, proximate composition and starch content significantly decreased (p < 0.05), while total carbohydrates, β-glucan, and energy value significantly increased (p < 0.05). Significant decreases in pH and alcoholic acidity (pH = 0.55) and 0.33% DM were observed. Total phenol content and antioxidant activity decreased to 5.57 mg GAE/g DM TPC, 22.20 μmol Fe2+/g DM FRAP, and 8.12 μmol TE/g DM DPPH during storage (p < 0.05). Of the 15 phytochemical compounds, gallic, p-coumaric, trans-cinnamic acids, and epicatechin were highly abundant in this buckwheat variety, with a notable 38% decrease in epicatechin. Dihydrocaffeic and phloretic acids, daidzein, naringin, and naringenin were also quantified in buckwheat. Its easy adaptability to the environment, ability to attract various insects, being a speedy short-season growing plant for food, and numerous nutritional and health benefits give buckwheat the potential to be a sustainable and biodiversity-friendly crop.
Development and Application of Hydrogen-Based Direct Reduction Iron Process
Authors: Ji Yuzhang , Chi Zhongyuan , Yuan Shufu , Chen Yongxu , Li Yujie , Jiang Tianchi , Liu Xin , Zhang Weijun
Published: August 2024

The conventional iron and steel industry (ISI), driven by coal utilization as its predominant feedstock, constitutes a substantial source of greenhouse gas emissions. Hydrogen metallurgy presents the opportunity to mitigate carbon emissions in ISI from the origin. Among hydrogen metallurgical approaches, the hydrogen-based direct reduction iron (H-DRI) process stands out for its substantial carbon reduction capabilities and established technological maturity. The present paper provides a comprehensive review of the development and application surrounding the H-DRI process. Firstly, the main chemical reactions of H-DRI and the relevant important parameters are introduced. Subsequently, an overview is provided of several prominent H-DRI processes, including HYL, Midrex, Midrex-H2®, HYL-III, HYL-ZR, BL, and Finmet, elucidating their characteristics through comparative analysis. Moreover, some research results of H-DRI process optimization are summarized. Leveraging insights garnered from globally representative projects exemplifying the industrial deployment of H-DRI technology in recent years, the trajectory of and prospective trends for industrial development in the field of H-DRI processes are explored. Further, prevailing challenges and impediments encountered in the adoption of H-DRI processes are identified, culminating in strategic recommendations tailored towards fostering future advancements. In the long term, the H-DRI process is expected to become a key path to achieve ISI cleaner production.
Research on Defect Diagnosis of Transmission Lines Based on Multi-Strategy Image Processing and Improved Deep Network
Authors: Gou Ming , Tang Hao , Song Lei , Chen Zhong , Yan Xiaoming , Zeng Xiangwen , Fu Wenlong
Published: August 2024

The current manual inspection of transmission line images captured by unmanned aerial vehicles (UAVs) is not only time-consuming and labor-intensive but also prone to high rates of false detections and missed inspections. With the development of artificial intelligence, deep learning-based image recognition methods can automatically detect various defect categories of transmission lines based on images captured by UAVs. However, existing methods are often constrained by incomplete feature extraction and imbalanced sample categories, which limit the precision of detection. To address these issues, a novel method based on multi-strategy image processing and an improved deep network is proposed to conduct defect diagnosis of transmission lines. Firstly, multi-strategy image processing is proposed to extract the effective area of transmission lines. Then, a generative adversarial network is employed to generate images of transmission lines to enhance the trained samples’ diversity. Finally, the deep network GoogLeNet is improved by superseding the original cross-entropy loss function with a focal loss function to achieve the deep feature extraction of images and defect diagnosis of transmission lines. An actual imbalance transmission line dataset including normal, broken strands, and loose strands is applied to validate the effectiveness of the proposed method. The experimental results, as well as contrastive analysis, reveal that the proposed method is suitable for recognizing defects of transmission lines.
A High-Resolution Distribution Dataset of Paddy Rice in India Based on Satellite Data
Authors: Chen Xuebing , Shen Ruoque , Pan Baihong , Peng Qiongyan , Zhang Xi , Fu Yangyang , Yuan Wenping
Published: August 2024

India, as the world’s second-largest rice producer, accounting for 21.7% of global rice production, plays a crucial role in ensuring global food supply stability. However, creating high-resolution rice maps for India, such as those at 10 to 30 m, poses significant challenges due to frequent cloudy weather conditions and the complexities of its agricultural systems. This study used a sample-independent mapping method for rice in India using the synthetic aperture radar (SAR)-based Rice Index (SPRI). We produced 10 m spatial resolution rice distribution maps for three years (i.e., 2018, 2020, and 2022) for 23 states in India, covering 98% of Indian rice production. The method effectively utilized the unique characteristics of rice in the vertical–horizontal (VH) backscatter coefficient time series of Sentinel-1, from ttransplantation to the maturity stage, combined with cloud-free Sentinel-2 imagery. By calculating the SPRI values for each agricultural field object using adaptive parameters, the planting locations of rice were accurately identified. On average, the user, producer, and overall accuracy over all investigated states and union territories was 84.72%, 82.31%, and 84.40%, respectively. Additionally, the regional-scale validation based on the statistical area at the district level showed that the coefficient of determination (R2) ranged from 0.53 to 0.95 for each state, indicating that the spatial distribution of the statistical planted area at the district level was reproduced well.
Towards Optimising the Derivation of Phenological Phases of Different Crop Types over Germany Using Satellite Image Time Series
Authors: Htitiou Abdelaziz , Möller Markus , Riedel Tanja , Beyer Florian , Gerighausen Heike
Published: August 2024

Operational crop monitoring applications, including crop type mapping, condition monitoring, and yield estimation, would benefit from the ability to robustly detect and map crop phenology measures related to the crop calendar and management activities like emergence, stem elongation, and harvest timing. However, this has proven to be challenging due to two main issues: first, the lack of optimised approaches for accurate crop phenology retrievals, and second, the cloud cover during the crop growth period, which hampers the use of optical data. Hence, in the current study, we outline a novel calibration procedure that optimises the settings to produce high-quality NDVI time series as well as the thresholds for retrieving the start of the season (SOS) and end of the season (EOS) of different crops, making them more comparable and related to ground crop phenological measures. As a first step, we introduce a new method, termed UE-WS, to reconstruct high-quality NDVI time series data by integrating a robust upper envelope detection technique with the Whittaker smoothing filter. The experimental results demonstrate that the new method can achieve satisfactory performance in reducing noise in the original NDVI time series and producing high-quality NDVI profiles. As a second step, a threshold optimisation approach was carried out for each phenophase of three crops (winter wheat, corn, and sugarbeet) using an optimisation framework, primarily leveraging the state-of-the-art hyperparameter optimization method (Optuna) by first narrowing down the search space for the threshold parameter and then applying a grid search to pinpoint the optimal value within this refined range. This process focused on minimising the error between the satellite-derived and observed days of the year (DOY) based on data from the German Meteorological Service (DWD) covering two years (2019–2020) and three federal states in Germany. The results of the calculation of the median of the temporal difference between the DOY observations of DWD phenology held out from a separate year (2021) and those derived from satellite data reveal that it typically ranged within ±10 days for almost all phenological phases. The validation results of the detection of dates of phenological phases against separate field-based phenological observations resulted in an RMSE of less than 10 days and an R-squared value of approximately 0.9 or greater. The findings demonstrate how optimising the thresholds required for deriving crop-specific phenophases using high-quality NDVI time series data could produce timely and spatially explicit phenological information at the field and crop levels.