bluuebunny/crossref_metadata_2025 huggingface dataset:
How Weird is The Worm? Evolution of the Developmental Gene Toolkit in Caenorhabditis elegans
Authors: Baker Emily A. , Woollard Alison
Published: September 2019

Comparative developmental biology and comparative genomics are the cornerstones of evolutionary developmental biology. Decades of fruitful research using nematodes have produced detailed accounts of the developmental and genomic variation in the nematode phylum. Evolutionary developmental biologists are now utilising these data as a tool with which to interrogate the evolutionary basis for the similarities and differences observed in Nematoda. Nematodes have often seemed atypical compared to the rest of the animal kingdom—from their totally lineage-dependent mode of embryogenesis to their abandonment of key toolkit genes usually deployed by bilaterians for proper development—worms are notorious rule breakers of the bilaterian handbook. However, exploring the nature of these deviations is providing answers to some of the biggest questions about the evolution of animal development. For example, why is the evolvability of each embryonic stage not the same? Why can evolution sometimes tolerate the loss of genes involved in key developmental events? Lastly, why does natural selection act to radically diverge toolkit genes in number and sequence in certain taxa? In answering these questions, insight is not only being provided about the evolution of nematodes, but of all metazoans.
The Impact of the Economic Recession in Greece on Assisted Reproduction Demand: A Retrospective Longitudinal Study
Authors: Sfakianoudis Konstantinos , Simopoulou Mara , Rapani Anna , Grigoriadis Sokratis , Maziotis Evangelos , Giannelou Polina , Pantou Agni , Vaxevanoglou Terpsithea , Fakiridou Maria , Koutsilieris Michael , Pantos Konstantinos
Published: September 2019

Background and objectives: Since 2009, the decline of the Greek economy has been in the spotlight of the world’s financial agenda. This study assesses the economic crisis’ impact on assisted reproduction demand dynamics. Materials and Methods: Patient records were recruited between 2005–2017. In 2013 the clinic proceeded with a cost reduction. The studied time-frames were defined accordingly: Period A: Prior to economic crisis, Period B: During the economic crisis-prior to cost reduction, and Period C: During the economic crisis-following cost reduction. Analysis focused on parameters reflecting on patient characteristics, decision to inquire on IVF treatment, embarking on it, and proceeding with multiple cycles. Results: The mean annual number of first visit patients was significantly decreased during Period B (1467.00 ± 119.21) in comparison to period A (1644.40 ± 42.57) and C (1637.8 ± 77.23). Furthermore, Period C in comparison to B and A, refers to women of more advanced age (37.27 ± 5.62 vs 36.04 ± 5.76 vs 35.53 ± 5.28), reporting a longer infertility period (8.49 ± 6.25 vs 7.01 ± 5.66 vs 6.46 ± 5.20), being inclined to abandon IVF treatment sooner (2.78 ± 2.51 vs 2.60 ± 1.92 vs 4.91 ± 2.28). Conclusions: A decline regarding assisted reproduction techniques (ART) demand was noted as anticipated. Redefining the cost of infertility treatments may contribute towards overcoming the troubling phenomenon of postponing pregnancy due to financial concerns.
Autologous Fat Grafting for Craniofacial Reconstruction in Oncologic Patients
Authors: Drochioi Cristian Ilie , Sulea Daniela , Timofte Daniel , Mocanu Veronica , Popescu Eugenia , Costan Victor Vlad
Published: September 2019

Due to the anatomical and functional complexity of the region, craniofacial tumor removal requires some of the most challenging surgical approaches, often complemented with advanced chemo-radiotherapy techniques. However, these modern therapies often lead to sequelae that can drastically reduce the quality of life for the surviving patients. Recent advances in the field of regenerative medicine opened new avenues for craniofacial reconstruction following head and neck cancer treatment. One of the most promising recent strategies relies on the use of autologous fat transplant. In this mini review, we briefly present some of the fat’s biological properties that make it an ideal tissue for craniofacial reconstruction following cancer treatment. We then outline the recent advances that led to a better understanding of the detailed anatomy of the craniofacial fat depots. Furthermore, we provide a succinct review of the methods used for fat harvesting, processing and engrafting in the craniofacial area after head and neck tumor removal, discussing their main applications, advantages and limitations.
The Discrimination Against, Health Status and Wellness of People Who Use Drugs in Italian Services: A Survey
Authors: Pelullo Concetta Paola , Curcio Fabio , Auriemma Francesco , Cefalo Giuseppe , Fabozzi Antonio , Rossiello Riccardo , Spagnoli Laura , Attena Francesco
Published: September 2019

Background and Objectives: The aims of this study are to: describe the sociodemographic characteristics and typology of drug addiction among people who use drugs that attend the Servizio per le Dipendenze (SerD), and evaluate the competence and ability of these rehabilitation services to improve their health status and wellness. Materials and Methods: A cross-sectional study was conducted from January to July 2017. Patients attending two selected SerD facilities in the city of Naples, Italy were interviewed with a questionnaire gathering information on sociodemographic data, characteristics of drug addiction, characteristics of enrolment at the SerD, self-reported health status and wellness, and reports of the discrimination suffered. Results: Among the 451 people interviewed, 72.3% had started taking drugs by the age of 20, and half of them have used drugs within the last year. 54.5% of responders attended SerD for more than 10 years, and the two main reasons for attendance were to get help and to get methadone. 79.4% were declared to have a good/very good/excellent health status at the time of interviewing. 53.7% reported suffering from discrimination. Conclusions: Based on our study, discrimination is higher in participants who attended SerD for more than one year, who were formerly in prison, or who were current drug users.
Bioelectric Impedance Vector Analysis (BIVA) in Breast Cancer Patients: A Tool for Research and Clinical Practice
Authors: Limon-Miro Ana Teresa , Valencia Mauro E. , Lopez-Teros Veronica , Guzman-Leon Alan Eduardo , Mendivil-Alvarado Herminia , Astiazaran-Garcia Humberto
Published: September 2019

Background and objectives: Body composition assessment can provide information associated with breast cancer patients’ (BCP) prognosis, that can lead interventions to improve survival outcomes. The aim of this study was to evaluate the effect of an individualized nutrition intervention program on breast cancer patients using bioelectrical impedance vector analysis (BIVA). Materials and Methods: This is a pretest-posttest study in recently diagnosed nonmetastatic BCP undergoing antineoplastic treatment, free of co-morbidities and dietary supplementation. Body composition was assessed at baseline and 6 months after an individualized nutrition intervention program, by dual-energy X-ray absorptiometry and BIVA. According to BIVA, each participant was located in the bivariate tolerance ellipses for Mexican population (50%, 75%, and 95%). In clinical practice, the 50% and 75% ellipses are considered within normality ranges. Results: Nine nonmetastatic BCP completed the intervention and were included in the analysis. After the intervention, they decreased by 5.8 kg of body weight (IQR, 3–6; p < 0.05), 3.8 kg of fat mass (IQR, 0.1–4.2; p < 0.05), and 1.4 kg of fat-free mass (IQR, −0.1 to 4; p < 0.05) while appendicular skeletal muscle mass remained unchanged (−0.2 kg, IQR, −0.8 to 2.3; p = 0.4). Using BIVA at baseline, five participants were among the 50% and 75% ellipses, mainly located in the area corresponding to edema and low lean tissue, two in the cachexia quadrant and two in the athletic quadrant (≥95% ellipse). After 6 months of intervention, six out of nine participants were in the athletic quadrant and eight of nine BCP were above the 5° phase angle cut-off point. One patient initially presented cachexia (≥95% ellipse); at postintervention her vector changed to the 50% ellipse. Conclusions: An individualized nutrition intervention program designed for nonmetastatic BCP was effective to improve the nutritional status of BCP as assessed by BIVA, therefore BIVA can be a useful tool to monitor changes in nonmetastatic BCP body composition in research and clinical practice.
On Phenomenological Failure Loci of Metals under Constant Stress States of Combined Tension and Shear: Issues of Coaxiality and Non-Uniqueness
Authors: Butcher Cliff , Abedini Armin
Published: September 2019

The present study investigates how the choice of characterization test and the composition of the stress state in terms of tension and shear can produce a non-unique failure locus in terms of stress triaxiality under plane stress conditions. Stress states that are composed of tensile and simple shear loadings result in a loss of proportionality between the cumulative strain and stress such that the principal frames become non-coaxial despite a constant stress triaxiality. Consequently, it is shown that the conventional interpretation of a failure locus in plane stress is based upon an implicit assumption of proportional coaxial loading. The use of simple shear tests along with traditional in-plane tensile tests for fracture characterization is only one “path” that can be taken in terms of the stress triaxiality, which may produce a bifurcation at uniaxial tension while the tension–torsion path does not. In general, the failure locus in terms of the equivalent strain is a failure surface and must consider the composition of the stress state that produces a given triaxiality. A comprehensive review of phenomenological fracture loci within a modified Mohr-Coulomb (MMC) framework is performed to highlight how the choice of stress states obtained using different characterization tests can change the apparent fracture locus of a material. The finite strain solutions for the work conjugate equivalent strain are derived for various loading paths that produce the same stress triaxiality. It is then shown that accounting for non-coaxiality leads to equivalent failure strains that are even higher than previously reported in tension–torsion tests within the literature. The equivalent plastic strains integrated from finite-element simulations are work-conjugate by definition. The equivalent strains estimated from the cumulative principal strains using DIC strain measurement depend upon a coaxial or non-coaxial assumption. Finally, an analytical solution for the onset of diffuse necking that accounts for the stabilizing influence of shear loading against a tensile instability is considered. Even under plane stress conditions, a failure surface arises in terms of the equivalent strain at necking, the stress triaxiality, and the severity of shear loading.
Physical Metallurgy of High Manganese Steels
Authors: Bleck Wolfgang , Haase Christian
Published: September 2019

The development of materials with advanced or new properties has been the primary aim of materials scientists for past centuries [...]
Modernization and Optimization of Phosphoric Cast Iron Casting
Authors: Kurylo Piotr
Published: September 2019

The article presents a detailed analysis of technical and organizational problems of modernization of a phosphoric cast iron casting process. The paper indicates the need to optimize the process of phosphoric cast iron casting, and the analysis carried out in the article about the possibility of optimization of the casting process allowed us to determine the main direction of modernization of the process, oriented on a significant increase in the quality of the cast iron, as well as in the direction of a significant increase in the efficiency of the cast iron production process.
Efficient Synthesis of Alkali Borohydrides from Mechanochemical Reduction of Borates Using Magnesium–Aluminum-Based Waste
Authors: Le Thi Thu , Pistidda Claudio , Puszkiel Julián , Milanese Chiara , Garroni Sebastiano , Emmler Thomas , Capurso Giovanni , Gizer Gökhan , Klassen Thomas , Dornheim Martin
Published: September 2019

Lithium borohydride (LiBH4) and sodium borohydride (NaBH4) were synthesized via mechanical milling of LiBO2, and NaBO2 with Mg–Al-based waste under controlled gaseous atmosphere conditions. Following this approach, the results herein presented indicate that LiBH4 and NaBH4 can be formed with a high conversion yield starting from the anhydrous borates under 70 bar H2. Interestingly, NaBH4 can also be obtained with a high conversion yield by milling NaBO2·4H2O and Mg–Al-based waste under an argon atmosphere. Under optimized molar ratios of the starting materials and milling parameters, NaBH4 and LiBH4 were obtained with conversion ratios higher than 99.5%. Based on the collected experimental results, the influence of the milling energy and the correlation with the final yields were also discussed.
Finite Element Analysis of Forward Slip in Micro Flexible Rolling of Thin Aluminium Strips
Authors: Qu Feijun , Xu Jianzhong , Jiang Zhengyi
Published: September 2019

This study delineates a novel finite element model to consider a pattern of process parameters affecting the forward slip in micro flexible rolling, which focuses on the thickness transition area of the rolled strip with thickness in the micrometre range. According to the strip marking method, the forward slip is obtained by comparison between the distance of the bumped ridges on the roll and that of the markings indented by the ridges, which not only simplifies the calculation process, but also maintains the accuracy as compared with theoretical estimates. The simulation results identify the qualitative and quantitative variations of forward slip with regard to the variations in the reduction, rolling speed, estimated friction coefficient and the ratio of strip thickness to grain size, respectively, which also locate the cases wherein the relative sliding happens between the strip and the roll. The developed grain-based finite element model featuring 3D Voronoi tessellations allows for the investigation of the scatter effect of forward slip, which gets strengthened by the enhanced effect of every single grain attributed to the dispersion of fewer grains in a thinner strip with respect to constant grain size. The multilinear regression analysis is performed to establish a statistical model based upon the simulation results, which has been proven to be accurate in quantitatively describing the relationship between the forward slip and the aforementioned process parameters by considering both correlation and error analyses. The magnitudes of each process parameter affecting forward slip are also determined by variance analysis.
An In Vivo Evaluation of Biocompatibility and Implant Accuracy of the Electron Beam Melting and Commercial Reconstruction Plates
Authors: Moiduddin Khaja , Hammad Mian Syed , Alkindi Mohammed , Ramalingam Sundar , Alkhalefah Hisham , Alghamdi Osama
Published: September 2019

The use of additive manufacturing in medical applications has become more prevalent over the last decade. Studies have proved that reconstruction plates with a mesh structure enhance the biocompatibility and bone-ingrowth formation. However, limited studies have been reported in the customization and in vivo clinical assessment of mesh implants. The purpose of this study was to investigate the surgical treatment and implant fitting accuracy using three different reconstruction plates. Fifteen goats were divided into one control and three experimental groups (Groups 1, 2, and 3) with five in each group. An experimental segmental defect was created on these animals and was adopted with customized electron beam melting reconstruction titanium plates with mesh in Group 1 and without mesh in Group 2 and commercial reconstruction plate in Group 3. All the animals were subjected to radiographic analysis before and after surgery. The subjected animals were sacrificed after 3 months and the electron beam melting reconstruction plates were compared with the commercial plate based on clinical and histology analysis and implant fitting accuracy. Both the electron beam melting reconstruction plates (with mesh and without mesh) and commercial plates survived the three months post-operation, revealing good wound-healing with new bone formation and without any foreign-body reaction. The electron beam melting reconstructed plate with mesh (Group 1) was found to have a better implant fitting when compared to the other two groups. The average discrepancy between Groups 2 and 3 was not significant. Certainly, the commercial plate (Group 3) was found to have the least accuracy as compared to other electron beam melting reconstruction plates (Group 1 and Group 2). Custom design electron beam melting fabricated reconstruction plates possessed better functionality, aesthetic outcome, and long-term biocompatibility when compared to commercial plates. Animal results indicated that the electron beam melting plates with mesh (Group 1) were superior in comparison to the other two groups due to its ability to provide better bone-in-growth and osseointegration on its porous microstructure.
The Effects of Doxorubicin-based Chemotherapy and Omega-3 Supplementation on Mouse Brain Lipids
Authors: Bennouna Djawed , Solano Melissa , Orchard Tonya S. , DeVries A. Courtney , Lustberg Maryam , Kopec Rachel E.
Published: September 2019

Chemotherapy-induced cognitive impairment affects ~30% of breast cancer survivors, but the effects on how chemotherapy impacts brain lipids, and how omega-3 polyunsaturated fatty acid supplementation may confer protection, is unknown. Ovariectomized mice were randomized to two rounds of injections of doxorubicin + cyclophosphamide or vehicle after consuming a diet supplemented with 2% or 0% EPA+DHA, and sacrificed 4, 7, and 14 days after the last injection (study 1, n = 120) or sacrificed 10 days after the last injection (study 2, n = 40). Study 1 whole brain samples were extracted and analyzed by UHPLC-MS/MS to quantify specialized pro-resolving mediators (SPMs). Lipidomics analyses were performed on hippocampal extracts from study 2 to determine changes in the brain lipidome. Study 1 results: only resolvin D1 was present in all samples, but no differences in concentration were observed (P > 0.05). Study 2 results: chemotherapy was positively correlated with omega-9 fatty acids, and EPA+DHA supplementation helped to maintain levels of plasmalogens. No statistically significant chemotherapy*diet effect was observed. Results demonstrate a limited role of SPMs in the brain post-chemotherapy, but a significant alteration of hippocampal lipids previously associated with other models of cognitive impairment (i.e., Alzheimer’s and Parkinson’s disease).
2D Materials in Development of Electrochemical Point-of-Care Cancer Screening Devices
Authors: Mohammadniaei Mohsen , Nguyen Huynh Vu , Tieu My Van , Lee Min-Ho
Published: September 2019

Effective cancer treatment requires early detection and monitoring the development progress in a simple and affordable manner. Point-of care (POC) screening can provide a portable and inexpensive tool for the end-users to conveniently operate test and screen their health conditions without the necessity of special skills. Electrochemical methods hold great potential for clinical analysis of variety of chemicals and substances as well as cancer biomarkers due to their low cost, high sensitivity, multiplex detection ability, and miniaturization aptitude. Advances in two-dimensional (2D) material-based electrochemical biosensors/sensors are accelerating the performance of conventional devices toward more practical approaches. Here, recent trends in the development of 2D material-based electrochemical biosensors/sensors, as the next generation of POC cancer screening tools, are summarized. Three cancer biomarker categories, including proteins, nucleic acids, and some small molecules, will be considered. Various 2D materials will be introduced and their biomedical applications and electrochemical properties will be given. The role of 2D materials in improving the performance of electrochemical sensing mechanisms as well as the pros and cons of current sensors as the prospective devices for POC screening will be emphasized. Finally, the future scopes of implementing 2D materials in electrochemical POC cancer diagnostics for the clinical translation will be discussed.
Design of a Monolithic Double-Slider Based Compliant Gripper with Large Displacement and Anti-Buckling Ability
Authors: Hao Guangbo , Zhu Jiaxiang
Published: September 2019

In a micro-manipulation system, the compliant gripper is used for gripping, handling and assembling of objects. Large displacement and anti-buckling characteristics are desired in the design of the gripper. In this paper, a compliant gripper with these two characteristics is proposed, modelled and verified. The large displacement is enabled by using distributed compliance in a double-slider kinematic mechanism. An inverted flexure arrangement enables the anti-buckling of the gripper when closing the two jaws. A pseudo-rigid-body model (PRBM) method with the help of virtual work principle is employed to obtain several desired analytical relations including the amplification coefficient and kinetostatics. The results of the finite element analysis (FEA) are shown to be consistent with the results of the derived analytical model. An experimental test was carried out through a milling machined aluminium alloy prototype, the results of which verify the good performance of the compliant gripper.
Effect of Mechanical Compression on Invasion Process of Malignant Melanoma Using In Vitro Three-Dimensional Cell Culture Device
Authors: Morikura Takashi , Miyata Shogo
Published: September 2019

Malignant melanoma in the plantar surface of the foot is subjected to various mechanical stimuli generated by daily human activity such as walking. Some studies have reported that mechanical compression affects the development and progression of melanoma. However, little is known about how mechanical compression affects the behavior of malignant melanoma cells in a physiological condition due to the complexity of the invasion mechanisms. In this study, we developed an in vitro three-dimensional cell culture device using microporous membrane in order to evaluate the effects of mechanical compression on the invasion process of malignant melanoma. Our results suggest that the invasion of melanoma cells under the compressive stress for 8 h of culture was promoted with the elongation of F-actin filaments compared to control groups, whereas there was no significant difference between both groups at 32 h of culture, with increasing cell death associated with promoting melanin synthesis. The results of this study contribute to the elucidation of the invasion mechanisms of malignant melanoma caused by mechanical stimulation.
Bacterial IAA-Delivery into Medicago Root Nodules Triggers a Balanced Stimulation of C and N Metabolism Leading to a Biomass Increase
Authors: Defez Roberto , Andreozzi Anna , Romano Silvia , Pocsfalvi Gabriella , Fiume Immacolata , Esposito Roberta , Angelini Claudia , Bianco Carmen
Published: September 2019

Indole-3-acetic acid (IAA) is the main auxin acting as a phytohormone in many plant developmental processes. The ability to synthesize IAA is widely associated with plant growth-promoting rhizobacteria (PGPR). Several studies have been published on the potential application of PGPR to improve plant growth through the enhancement of their main metabolic processes. In this study, the IAA-overproducing Ensifer meliloti strain RD64 and its parental strain 1021 were used to inoculate Medicago sativa plants. After verifying that the endogenous biosynthesis of IAA did not lead to genomic changes during the initial phases of the symbiotic process, we analyzed whether the overproduction of bacterial IAA inside root nodules influenced, in a coordinated manner, the activity of the nitrogen-fixing apparatus and the photosynthetic function, which are the two processes playing a key role in legume plant growth and productivity. Higher nitrogen-fixing activity and a greater amount of total nitrogen (N), carbon (C), Rubisco, nitrogen-rich amino acids, soluble sugars, and organic acids were measured for RD64-nodulated plants compared to the plants nodulated by the wild-type strain 1021. Furthermore, the RD64-nodulated plants showed a biomass increase over time, with the highest increment (more than 60%) being reached at six weeks after infection. Our findings show that the RD64-nodulated plants need more substrate derived from photosynthesis to generate the ATP required for their increased nitrogenase activity. This high carbohydrate demand further stimulates the photosynthetic function with the production of molecules that can be used to promote plant growth. We thus speculate that the use of PGPR able to stimulate both C and N metabolism with a balanced C/N ratio represents an efficient strategy to obtain substantial gains in plant productivity.
Experimental In-Field Transfer and Survival of Escherichia coli from Animal Feces to Romaine Lettuce in Salinas Valley, California
Authors: Jeamsripong Saharuetai , Chase Jennifer A. , Jay-Russell Michele T. , Buchanan Robert L. , Atwill Edward R.
Published: September 2019

This randomized controlled trial characterized the transfer of E. coli from animal feces and/or furrow water onto adjacent heads of lettuce during foliar irrigation, and the subsequent survival of bacteria on the adaxial surface of lettuce leaves. Two experiments were conducted in Salinas Valley, California: (1) to quantify the transfer of indicator E. coli from chicken and rabbit fecal deposits placed in furrows to surrounding lettuce heads on raised beds, and (2) to quantify the survival of inoculated E. coli on Romaine lettuce over 10 days. E. coli was recovered from 97% (174/180) of lettuce heads to a maximal distance of 162.56 cm (5.33 ft) from feces. Distance from sprinklers to feces, cumulative foliar irrigation, and lettuce being located downwind of the fecal deposit were positively associated, while distance from fecal deposit to lettuce was negatively associated with E. coli transference. E. coli exhibited decimal reduction times of 2.2 and 2.5 days when applied on the adaxial surface of leaves within a chicken or rabbit fecal slurry, respectively. Foliar irrigation can transfer E. coli from feces located in a furrow onto adjacent heads of lettuce, likely due to the kinetic energy of irrigation droplets impacting the fecal surface and/or impacting furrow water contaminated with feces, with the magnitude of E. coli enumerated per head of lettuce influenced by the distance between lettuce and the fecal deposit, cumulative application of foliar irrigation, wind aspect of lettuce relative to feces, and time since final irrigation. Extending the time period between foliar irrigation and harvest, along with a 152.4 cm (5 ft) no-harvest buffer zone when animal fecal material is present, may substantially reduce the level of bacterial contamination on harvested lettuce.
Geochronology and Geochemistry of Ore-Hosting Rhyolitic Tuff in the Kengdenongshe Polymetallic Deposit in the Eastern Segment of the East Kunlun Orogen
Authors: Liang Ya-Yun , Xia Rui , Shan Xiaoyu , Ma Yao , Zhao En-Quan , Guo Wenhui
Published: September 2019

The Kengdenongshe Au–Ag–Pb–Zn polymetallic deposit, a newly discovered large-scale polymetallic deposit in the southeastern section of the East Kunlun Orogen (EKO), contains an Au resource of 40 t, Ag resource of 690 t and Pb–Zn resource of 10.5 × 105 t. The ore-bearing rocks are mainly composed of laminar barite and rhyolitic tuff. In this study, LA-ICP-MS zircon U–Pb dating and whole rock major and trace elements analyses have been conducted on the ore-bearing rhyolitic tuff. LA-ICP-MS zircon U–Pb dating data show that these rhyolitic tuffs were emplaced at ca. 243.3 ± 1.6 Ma. The samples show similar features to those of S-type granites, including high contents of SiO2 (76.4–82.6 wt. %) and Al2O3 (11.0–12.7 wt. %) and relatively low concentrations of Na2O (0.35–2.43 wt. %) and CaO (0.095–0.124 wt. %), with high A/CNK (molar [Al2O3/(CaO + Na2O + K2O)]) (1.72–2.03) and K2O/Na2O ratios (1.41–17.1). Further, they exhibit depletion in HFSEs (High Field Strength Elements) and enrichment in LREEs (Light Rare Earth Element) with negative Eu anomalies (Eu/Eu* = 0.51–0.64). These geochemical characteristics indicate that the Kengdenongcuo rhyolitic tuff originated from the fluid-absent melting of a plagioclase-poor, clay-rich metapelitic source and experienced minor fractional crystallization. In combination with arc-type magmatism and contemporaneous syn-collision granitoids in the region, the Kengdenongcuo tuff formed in a continental collision setting, implying that the Bayan Har–Songpan Ganzi Terrane collided with the East Kunlun Terrane and the Paleo-Tethys Ocean was closed at the period of ~243 Ma. The Kengdenongcuo polymetallic deposit formed at about the same time.
Mediating-Moderating Effect of Allostatic Load on the Association between Dietary Approaches to Stop Hypertension Diet and All-Cause and Cause-Specific Mortality: 2001–2010 National Health and Nutrition Examination Surveys
Authors: Beydoun Hind A. , Huang Shuyan , Beydoun May A. , Hossain Sharmin , Zonderman Alan B.
Published: September 2019

This secondary analysis of survey data examined mediating-moderating effects of allostatic load score (calculated using the Rodriquez method) on the association between nutrient-based Dietary Approaches to Stop Hypertension (DASH) diet score (Mellen Index) and the all-cause and cause-specific mortality risks among 11,630 adults ≥ 30 years of age from the 2001–2010 National Health and Nutrition Examination Surveys with no history of cardiovascular disease or cancer at baseline, and who were followed-up for ~9.35 years. Multivariable models were adjusted for demographic, socioeconomic, lifestyle, and health characteristics. All-cause, cardiovascular disease, and cancer-specific mortality rates were estimated at 6.5%, 1.1%, and 1.9%, respectively. The median DASH total score was 3.0 (range: 1–8) (with 78.3% scoring < 4.5), whereas the median allostatic load score was 3 (range: 0–9). The DASH diet, fiber, and magnesium were negatively correlated with allostatic load, whereas allostatic load predicted higher all-cause mortality, irrespective of the DASH diet. Whereas protein was protective, potassium increased all-cause mortality risk, irrespective of allostatic load. Potassium was protective against cardiovascular disease-specific mortality but was a risk factor for cancer-specific mortality. Although no moderating effects were observed, mediation by the allostatic load on cardiovascular disease-specific mortality was observed for DASH total score and selected component scores. Direct (but not indirect) effects of DASH through the allostatic load were observed for all-cause mortality, and no direct or indirect effects were observed for cancer-specific mortality. From a public health standpoint, the allostatic load may be a surrogate for the preventive effects of the DASH diet and its components on cardiovascular disease-specific mortality risk.
Glutathione and Nitric Oxide: Key Team Players in Use and Disuse of Skeletal Muscle
Authors: Baldelli Sara , Ciccarone Fabio , Limongi Dolores , Checconi Paola , Palamara Anna Teresa , Ciriolo Maria Rosa
Published: September 2019

Glutathione (GSH) is the main non-enzymatic antioxidant playing an important role in detoxification, signal transduction by modulation of protein thiols redox status and direct scavenging of radicals. The latter function is not only performed against reactive oxygen species (ROS) but GSH also has a fundamental role in buffering nitric oxide (NO), a physiologically-produced molecule having-multifaceted functions. The efficient rate of GSH synthesis and high levels of GSH-dependent enzymes are characteristic features of healthy skeletal muscle where, besides the canonical functions, it is also involved in muscle contraction regulation. Moreover, NO production in skeletal muscle is a direct consequence of contractile activity and influences several metabolic myocyte pathways under both physiological and pathological conditions. In this review, we will consider the homeostasis and intersection of GSH with NO and then we will restrict the discussion on their role in processes related to skeletal muscle function and degeneration.
Serum Concentration of Plant Sterol Oxidation Products (POP) Compared to Cholesterol Oxidation Products (COP) after Intake of Oxidized Plant Sterols: A Randomised, Placebo-Controlled, Double-Blind Dose–Response Pilot Study
Authors: Lin Yuguang , Koppenol Wieneke P. , Knol Diny , Vermeer Mario A. , Hiemstra Harry , Friedrichs Silvia , Lütjohann Dieter , Trautwein Elke A.
Published: September 2019

Plant sterols (PS) are oxidized to PS oxidation products (POP). This study quantified the change in serum POP compared to cholesterol oxidation products (COP) after the intake of increasing POP doses. This was a double-blind, randomized, placebo-controlled, dose‒response pilot study with healthy individuals in four groups (15 per group). The control group received products with no added PS or POP and treatment groups received daily 20–25 g margarine with added PS (mean 3 g/d) and two cookies (~28 g) for six weeks. Cookies delivered 8.7 (low-dose), 15.2 (medium-dose), or 37.2 (high-dose) mg/d POP. Fasting serum POP and COP were measured at the baseline, days 14, 28, and 42 in all participants and days 7, 21, and 35 in a subset. Sixty individuals completed the study; 52 were included in per protocol analysis. Serum POP increased with increasing POP intake and plateaued at dose >15 mg/d. Stabilized POP concentrations were (mean ± SD) 38.9 ± 6.9, 91.0 ± 27.9, 144.4 ± 37.9 and 203.0 ± 63.7 nmol/L, for control, low-, medium-, and high-dose POP groups, respectively. For all groups, the serum COP ranged from 213 to 262 nmol/L and the average POP/COP ratio was <1. Serum POP concentrations increased non-linearly, reaching stabilized concentrations in <7 days, and remained below COP concentrations after the intake of increasing POP doses.
Fecal Source Tracking in A Wastewater Treatment and Reclamation System Using Multiple Waterborne Gastroenteritis Viruses
Authors: Ji Zheng , Wang Xiaochang C. , Xu Limei , Zhang Chongmiao , Rong Cheng , Rachmadi Andri Taruna , Amarasiri Mohan , Okabe Satoshi , Funamizu Naoyuki , Sano Daisuke
Published: September 2019

Gastroenteritis viruses in wastewater reclamation systems can pose a major threat to public health. In this study, multiple gastroenteritis viruses were detected from wastewater to estimate the viral contamination sources in a wastewater treatment and reclamation system installed in a suburb of Xi’an city, China. Reverse transcription plus nested or semi-nested PCR, followed by sequencing and phylogenetic analysis, were used for detection and genotyping of noroviruses and rotaviruses. As a result, 91.7% (22/24) of raw sewage samples, 70.8% (17/24) of the wastewater samples treated by anaerobic/anoxic/oxic (A2O) process and 62.5% (15/24) of lake water samples were positive for at least one of target gastroenteritis viruses while all samples collected from membrane bioreactor effluent after free chlorine disinfection were negative. Sequence analyses of the PCR products revealed that epidemiologically minor strains of norovirus GI (GI/14) and GII (GII/13) were frequently detected in the system. Considering virus concentration in the disinfected MBR effluent which is used as the source of lake water is below the detection limit, these results indicate that artificial lake may be contaminated from sources other than the wastewater reclamation system, which may include aerosols, and there is a possible norovirus infection risk by exposure through reclaimed water usage and by onshore winds transporting aerosols containing norovirus.
Atypical Dermatophytosis in 12 North American Porcupines (Erethizon dorsatum) from the Northeastern United States 2010–2017
Authors: Needle David B. , Gibson Robert , Hollingshead Nicholas A. , Sidor Inga F. , Marra Nicholas J. , Rothenheber Derek , Thachil Anil J. , Stanhope Bryce J. , Stevens Brian A. , Ellis Julie C. , Spanswick Shelley , Murray Maureen , Goodman Laura B.
Published: September 2019

Twelve wild North American porcupines (Erethizon dorsatum) out of a total of 44 of this species examined in an 8-year period were diagnosed with dermatopathies while being cared for at two wildlife rehabilitation clinics. Biopsy and necropsy were performed on seven and five animals, respectively. Atypical dermatophytosis was diagnosed in all cases. Lesions consisted of diffuse severe epidermal hyperkeratosis and mild hyperplasia with mild lymphoplasmacytic dermatitis and no folliculitis. Dermatophytes were noted histologically as hyphae and spores in hair shafts, and follicular and epidermal keratin. Trichophyton sp. was grown in 5/6 animals where culture was performed, with a molecular diagnosis of Arthroderma benhamiae/Trichophyton mentagrophytes in these five cases. Metagenomic analysis of formalin-fixed paraffin-embedded tissue samples from three cases identified fungi from 17 orders in phyla Basidiomycota and Ascomycota. Alteration of therapy from ketaconazole, which was unsuccessful in four out of five early cases, to terbinafine or nitraconazole led to the resolution of disease and recovery to release in four subsequent animals. In all, six animals were euthanized or died due to dermatopathy, no cases resolved spontaneously, and six cases were resolved with therapy. The work we present demonstrates an atypical lesion and anatomical distribution due to dermatophytosis in a series of free-ranging wild porcupines and the successful development of novel techniques for extracting and sequencing nucleic acids from fungus in archival formalin-fixed paraffin-embedded animal tissue.
Alternative Experimental Models for Studying Influenza Proteins, Host–Virus Interactions and Anti-Influenza Drugs
Authors: Chua Sonja C. J. H. , Tan Hui Qing , Engelberg David , Lim Lina H. K.
Published: September 2019

Ninety years after the discovery of the virus causing the influenza disease, this malady remains one of the biggest public health threats to mankind. Currently available drugs and vaccines only partially reduce deaths and hospitalizations. Some of the reasons for this disturbing situation stem from the sophistication of the viral machinery, but another reason is the lack of a complete understanding of the molecular and physiological basis of viral infections and host–pathogen interactions. Even the functions of the influenza proteins, their mechanisms of action and interaction with host proteins have not been fully revealed. These questions have traditionally been studied in mammalian animal models, mainly ferrets and mice (as well as pigs and non-human primates) and in cell lines. Although obviously relevant as models to humans, these experimental systems are very complex and are not conveniently accessible to various genetic, molecular and biochemical approaches. The fact that influenza remains an unsolved problem, in combination with the limitations of the conventional experimental models, motivated increasing attempts to use the power of other models, such as low eukaryotes, including invertebrate, and primary cell cultures. In this review, we summarized the efforts to study influenza in yeast, Drosophila, zebrafish and primary human tissue cultures and the major contributions these studies have made toward a better understanding of the disease. We feel that these models are still under-utilized and we highlight the unique potential each model has for better comprehending virus–host interactions and viral protein function.
A Monolithically Integrated Laser-Photodetector Chip for On-Chip Photonic and Microwave Signal Generation
Authors: Qi Hefei , Chen Guangcan , Lu Dan , Zhao Lingjuan
Published: September 2019

An Indium-phosphide-based monolithically integrated photonic chip comprising of an amplified feedback laser (AFL) and a photodetector was designed and fabricated for on-chip photonic and microwave generation. Various waveforms including single tone, multi-tone, and chaotic signal generation were demonstrated by simply adjusting the injection currents applied to the controlling electrodes. The evolution dynamics of the photonic chip was characterized. Photonic microwave with frequency separation tunable from 26.3 GHz to 34 GHz, chaotic signal with standard bandwidth of 12 GHz were obtained. An optoelectronic oscillator (OEO) based on the integrated photonic chip was demonstrated without using any external electrical filter and photodetector. Tunable microwave outputs ranging from 25.5 to 26.4 GHz with single sideband (SSB) phase noise less than −90 dBc/Hz at a 10-kHz offset from the carrier frequency were realized.
Is the Responsiveness to Light Related to the Differences in Stem Straightness among Populations of Pinus pinaster?
Authors: Sierra-de-Grado Rosario , Pando Valentín , Martínez-Zurimendi Pablo , Moulia Bruno
Published: September 2019

Stem straightness is related to wood quality and yield. Although important genetic differences in stem straightness among the natural populations of Pinus pinaster are well established, the main drivers of these differences are not well known. Since the responses of trees to light are key ecological features that induce stem curvature, we hypothesized that populations with better straightness should exhibit lower photomorphogenetic and phototropic sensitivity. We compared three populations to identify the main processes driven by primary and secondary growth that explain their differences in response to light. One-year-old seedlings were grown under two treatments—direct sunlight and lateral light plus shade—for a period of 5 months. The length and the leaning of the stems were measured weekly. The asymmetry of radial growth and compression wood (CW) formation were analyzed in cross-sections. We found differences among the populations in photomorphogenetic and phototropic reactions. However, the population with straighter stems was not characterized by reduced sensitivity to light. Photo(gravi)tropic responses driven by primary growth and gravitropic responses driven by secondary growth explained the kinetics of the stem leaning and CW pattern. Asymmetric radial growth and CW formation did not contribute to the phototropic reactions.
Japanese and Bohemian Knotweeds as Sustainable Sources of Carotenoids
Authors: Metličar Valentina , Vovk Irena , Albreht Alen
Published: September 2019

Japanese knotweed (Fallopia japonica Houtt.) and Bohemian knotweed (Fallopia x bohemica) are invasive alien plant species, causing great global ecological and economic damage. Mechanical excavation of plant material represents an effective containment method, but it is not economically and environmentally sustainable as it produces an excessive amount of waste. Thus, practical uses of these plants are actively being sought. In this study, we explored the carotenoid profiles and carotenoid content of mature (green) and senescing leaves of both knotweeds. Both plants showed similar pigment profiles. By means of high performance thin-layer chromatography with densitometry and high performance liquid chromatography coupled to photodiode array and mass spectrometric detector, 11 carotenoids (and their derivatives) and 4 chlorophylls were identified in green leaves, whereas 16 distinct carotenoids (free carotenoids and xanthophyll esters) were found in senescing leaves. Total carotenoid content in green leaves of Japanese knotweed and Bohemian knotweed (378 and 260 mg of lutein equivalent (LE)/100 g dry weight (DW), respectively) was comparable to that of spinach (384 mg LE/100 g DW), a well-known rich source of carotenoids. A much lower total carotenoid content was found for senescing leaves of Japanese and Bohemian knotweed (67 and 70 mg LE/100 g DW, respectively). Thus, green leaves of both studied knotweeds represent a rich and sustainable natural source of bioactive carotenoids. Exploitation of these invaders for the production of high value-added products should consequently promote their mechanical control.
Scratch on Polymer Materials Using AFM Tip-Based Approach: A Review
Authors: Yan Yongda , Chang Shunyu , Wang Tong , Geng Yanquan
Published: September 2019

As a brand new nanomachining method, the tip-based nanomachining/nanoscratching (TBN) method has exhibited a powerful ability at machining on polymer materials and various structures have been achieved using this approach, ranging from the nanodot, nanogroove/channel, bundle to 2D/3D (three-dimensional) nanostructures. The TBN method is widely used due to its high precision, ease of use and low environmental requirements. First, the theoretical models of machining on polymer materials with a given tip using the TBN method are presented. Second, advances of nanostructures achieved by this method are given, including nanodots/nanodot arrays, a nanogroove/channel, 2D/3D nanostructures and bundles. In particular, a useful approach called the ultrasonic vibration-assisted method introduced to integrate with TBN method to reduce the wear of the tip is also reviewed, respectively. Third, the typical applications of the TBN method and the nanostructures achieved by it are summarized in detail. Finally, the existing shortcomings and future prospects of the TBN method are given. It is confirmed that this review will be helpful in learning about this method and push the technology toward industrialization.
Preparation and Properties of Carbon Fiber/Carbon Nanotube Wet-Laid Composites
Authors: Lee Suhyun , Ko Kwangduk , Youk Jiho , Lim Daeyoung , Jeong Wonyoung
Published: September 2019

In this study, carbon nanotubes (CNTs) were introduced into carbon fiber (CF) wet-laid composites as functional nano-fillers to fabricate multi-functional composites with improved mechanical, electrical, and thermal properties. It was considered that the wet-laid process was most suitable in order to introduce filler into brittle and rigid carbon fiber substrates, and we established the conditions of the process that could impart dispersibility and bonding between the fibers. We introduced polyamide 6 (PA6) short fiber, which is the same polymeric material as the stacking film, into carbon fiber and CNT mixture to enhance the binding interactions between carbon fiber and CNTs. Various types of CNT-reinforced carbon fiber wet-laid composites with PA6 short fibers were prepared, and the morphology, mechanical and electrical properties of the composites were estimated. As CNT was added to the carbon fiber nonwoven, the electrical conductivity increased by 500% but the tensile strength decreased slightly. By introducing short fibers of the same material as the matrix between CNT–CF wet-laid nonwovens, it was possible to find optimum conditions to increase the electrical conductivity while maintaining mechanical properties.