bluuebunny/crossref_metadata_2025 huggingface dataset:
Improving Bambara Groundnut Production: Insight Into the Role of Omics and Beneficial Bacteria
Authors: Ajilogba Caroline Fadeke , Olanrewaju Oluwaseyi Samuel , Babalola Olubukola Oluranti
Published: March 2022

With the rise in the world population, environmental hazards caused by chemical fertilizers, and a decrease in food supply due to global climate change, food security has become very pertinent. In addition, considerable parts of agriculture lands have been lost to urbanization. It has therefore been projected that at the present rate of population increase coupled with the other mentioned factors, available food will not be enough to feed the world. Hence, drastic approach is needed to improve agriculture output as well as human sustainability. Application of environmentally sustainable approach, such as the use of beneficial microbes, and improved breeding of underutilized legumes are one of the proposed sustainable ways of achieving food security. Microbiome-assisted breeding in underutilized legumes is an untapped area with great capabilities to improve food security. Furthermore, revolution in genomics adaptation to crop improvement has changed the approach from conventional breeding to more advanced genomic-assisted breeding on the host plant and its microbiome. The use of rhizobacteria is very important to improving crop yield, especially rhizobacteria from legumes like Bambara groundnut (BGN). BGN is an important legume in sub-Saharan Africa with high ability to tolerate drought and thrive well in marginalized soils. BGN and its interaction with various rhizobacteria in the soil could play a vital role in crop production and protection. This review focus on the importance of genomics application to BGN and its microbiome with the view of setting a potential blueprint for improved BGN breeding through integration of beneficial bacteria.
Expression Patterns and Functional Analysis of 11 E3 Ubiquitin Ligase Genes in Rice
Authors: Zhang Huijuan , Zheng Dewei , Song Fengming , Jiang Ming
Published: March 2022

E3 ubiquitin ligases are involved in many processes, regulating the response to biotic and abiotic stresses. In this study, 11 E3 ubiquitin ligase genes from Arabidopsis, which were hypothesized to function in response to biotic or abiotic stresses were selected, and the homologous genes in rice were found. Their functions were analyzed in rice. These 11 E3 ubiquitin ligase genes showed different patterns of expression under different treatments. The BMV:OsPUB39-infiltrated seedlings showed decreased resistance to Magnaporthe grisea (M. grisea) when compared with BMV:00-infiltrated seedlings, whereas the BMV:OsPUB34- and BMV:OsPUB33-infiltrated seedlings showed increased resistance. The involvement of these genes in the resistance against M. grisea may be attributed to the regulation of the accumulation of reactive oxygen species (ROS) and expression levels of defense-related genes. Seedlings infiltrated by BMV:OsATL69 showed decreased tolerance to drought stress, whereas BMV:OsPUB33-infiltraed seedlings showed increased tolerance, possibly through the regulation of proline content, sugar content, and expression of drought-responsive genes. BMV:OsATL32-infiltrated seedlings showed decreased tolerance to cold stress by regulating malondialdehyde (MDA) content and the expression of cold-responsive genes.
Massive Open Online Course Study Group: Interaction Patterns in Face-to-Face and Online (Facebook) Discussions
Authors: Chen Pin-Ju , Chen Yang-Hsueh
Published: March 2022

Interaction has been regarded as a key design component in online and distance learning. In this study, we convened a student-led, blended mode (face-to-face and online/Facebook discussions) massive open online course (MOOC) study group to facilitate interactions for learning. Multiple data, including voice recordings, one-on-one interviews, video recordings, and artifacts were collected and analyzed to detect patterns of interaction in both face-to-face and online/Facebook settings, as well as student perceptions of the blended MOOC study group. Findings indicated that, overall, the blended mode MOOC study group was helpful for promoting communication, providing help, resolving problems, and exchanging ideas and information among group members. Moreover, face-to-face meetings and online discussions both might have exerted their unique strengths and functions in different learning situations for different learners. We recommend future studies continue to explore the tenability of the blended mode MOOC study group in different contexts, subject areas, and age groups, as well as examining group dynamics and interactions that transform MOOC learning into interactive, motivating, and fulfilling journeys among study group members.
Entrepreneur Hubris, Organizational Ambidexterity, and Dynamic Capability Construction
Authors: Guo Yan , Huang Pei-Wen , Ciu Chu , Fang Shih-Chieh , Tsai Fu-Sheng
Published: March 2022

This paper demonstrated the influences of initiation, development, turn-down, and reinitiation of the dynamic capability of an entrepreneurial firm in the solar energy industry. The focus is on the impact of entrepreneurial hubris, which may affect the decision of ambidexterity that can vitalize dynamic capability. The findings indicate that, when the major decision maker (the founder entrepreneur) has the trait of hubris, the decision-making process may be overly arbitrary, and a decision of being exploratory or exploitative alone is likely to be made. On the contrary, when the founder entrepreneur is aware of the hubris and shares decisive power, the decision of being ambidextrous as a dynamic capability is more freely achieved. This paper contributes by discovery of the cognitive-based microfoundation of entrepreneurial ventures and linkage of such microfoundation to organizational ambidexterity.
Family Income, Parental Education and Chinese Preschoolers’ Cognitive School Readiness: Authoritative Parenting and Parental Involvement as Chain Mediators
Authors: Xia Xiaoying
Published: March 2022

This study examined the associations of family income and parental education with Chinese preschool children’s cognitive school readiness and the sequential mediating role of parenting style (i.e., authoritative parenting) and parental involvement in these relations. A total of 307 5–6 years old kindergarten children from Shanghai, China and their parents participated in the study. Using structural equation modeling method, the results indicated that parental education was directly related to children’s cognitive school readiness, while no direct relationship was found for family income. The link of parents’ education with children’s cognitive school readiness was sequentially mediated by authoritative parenting and home-based parental involvement. Authoritative parenting and parental involvement at home can be targeted by government administrators to effectively improve children’s cognitive school readiness skills. The theoretical and practical implications were discussed.
Shamed If You Do, Shamed If You Do Not: Group-Based Moral Emotions, Accountability, and Tolerance of Enemy Collateral Casualties
Authors: Schori-Eyal Noa , Sobol-Sarag Danit , Shuman Eric , Halperin Eran
Published: March 2022

Civilian casualties contribute to the perpetuation of intergroup conflicts through increased radicalization and hostilities, but little is known on the psychological processes that affect responses to outgroup civilian casualties. The goal of the present research was to explore two factors expected to lead group members to act more cautiously, thereby reducing civilian casualties: perceived accountability and forecast group-based moral emotions. In two studies, Jewish–Israeli civilians (Study 1) and soldiers (Study 2) were asked to forecast their group-based moral emotions in case of Palestinian (i.e., outgroup) civilian casualties, then exposed to accountability manipulations. Participants who expected to feel low levels of shame and were primed with accountability made more cautious decisions than those in the control condition. Participants who expected to feel high levels of shame were unaffected by accountability primes. Theoretical and practical implications regarding forecast moral emotions and accountability as an intervention in intergroup conflicts are discussed.
Re-creation After Business Failure: A Conceptual Model of the Mediating Role of Psychological Capital
Authors: De Hoe Roxane , Janssen Frank
Published: March 2022

In case of failure, entrepreneurs could endure various financial, psychological, and social costs. These intertwined costs could affect their learning from failure. All individuals do not react in the same way when dealing with adversity. Rather than focusing on (negative) consequences of business failure, we took a more positive approach by using the Conservation of Resources (COR) model theory to build our conceptual model. Psychological capital, which refers to“an individual’s positive psychological state of development characterized by high levels of self-efficacy, optimism, hope,and resilience,”could be considered as a resource to recover from entrepreneurial setbacks. We suggest that a high level of psychological capital plays a mediating role in the relationship between the negative consequences of failure and learning from failure. By learning from this experience, failed entrepreneurs will increase their intention to re-create a venture and pursue their entrepreneurial career. This theoretical research, by building a conceptual model based on resources, offers a more positive approach of entrepreneurial failure and investigates key psychological assets, such as psychological capital, that support the development of entrepreneurial resilience rather than the prevention of business failure.
Associations of Suicide Risk and Community Integration Among Patients With Treatment-Resistant Depression
Authors: Huong Pham Thi Thu , Wu Chia-Yi , Lee Ming-Been , Chen I-Ming
Published: March 2022

Introduction

Treatment-resistant depression (TRD) is one of the primary causes of disability and a major risk for suicide among patients living in the community. However, the suicide risks and care needs for safety among patients with TRD during the community reintegration process appear to be underestimated. This study aimed to investigate the association between community integration and suicide risks among patients with treatment-resistant depression (TRD) with sub-analysis by gender.

Methods

Patients diagnosed with major depressive disorder were recruited upon psychiatrists' referral in two general hospitals in northern Taiwan during 2018–2019. The participants who experienced more than two failed treatments of antidepressants with partial remission were defined as TRD. A structured questionnaire was used to collect socio-demographic, suicidality, and psychosocial information.

Results

In a total of 125 participants, gender difference was identified in certain community integration aspects such as home integration, productivity, and electronic social networking. The male participants appeared to have better involvement in social contact with internet but slightly less video link than women, while women had higher level of home integration in the past month. The participants who performed worse in the social integration and better home-based activity or productivity levels had higher suicide risks including suicide ideation and overall suicide risks.

Conclusions

Community integration levels of home, social, and productivity were associated with suicidality in terms of overall suicide risk and recent suicide ideation. Facilitation of community integration at home and life arrangements might reduce suicide risks in TRD patients.
Maternal Deprivation Increased Vulnerability to Depression in Adult Rats Through DRD2 Promoter Methylation in the Ventral Tegmental Area
Authors: Guo Zhenli , Li Shansi , Wu Jialing , Zhu Xiongzhao , Zhang Yi
Published: March 2022

Objective

Early life adversity is a risk factor for depression in adulthood; however, the underlying mechanisms are not well understood. This study aims to investigate the effect of DNA methylation of DRD2 gene on early life stress–induced depression in adult rats.

Methods

Newborn Sprague–Dawley rats were randomly assigned to four groups: maternal deprivation group (MD), chronic unpredictable stress (CUS) group, maternal deprivation plus chronic unpredictable stress (MD/CUS) group, and normal control group (NOR). Behaviors were measured by open field test (OFT), sucrose preference test (SPT), and Original Research Article forced swimming test (FST). Fecal CORT level was detected by ELISA. Bisulfite amplicon sequencing PCR was used to assess methylation levels of DRD2 promoter.

Results

CUS and MD/CUS rats had a significantly shorter total distance, longer immobility time, and higher CORT level, while MD and MD/CUS rats had a significantly lower percentage of central distance, more feces, lower rate of sucrose preference, and lower levels of DRD2 protein and mRNA in the VTA than NOR rats. CUS rats showed a significantly higher DRD2 mRNA and protein levels in the VTA than NOR rats. CUS, MD, and MD/CUS rats showed a significantly higher level of DRD2 promoter methylation than NOR rats. CORT level was significantly correlated with the sucrose preference rate in SPT, the immobility time in FST, the total distance, and the number of fecal pellets in OFT. DRD2 protein level was significantly correlated with the sucrose preference rate and the number of fecal pellets. DRD2 mRNA level was significantly correlated with the percentage of central distance and the number of fecal pellets in OFT. The level of DRD2 promoter methylation was significantly correlated with the sucrose preference rate, immobility time, total distance, the percentage of central distance, and the number of fecal pellets.

Conclusions

Early life MD increased vulnerability to stress-induced depressive-like behavior in adult rats. Enhanced DRD2 promoter methylation in the VTA may increase the susceptibility to depression.
Dual-Task Treadmill Training for the Prevention of Falls in Parkinson's Disease: Rationale and Study Design
Authors: Mylius Veit , Maes Laura , Negele Katrin , Schmid Christine , Sylvester Ramona , Brook Caroline Sharon , Brugger Florian , Perez-Lloret Santiago , Bansi Jens , Aminian Kamiar , Paraschiv-Ionescu Anisoara , Gonzenbach Roman , Brugger Peter
Published: March 2022

Various factors, such as fear of falling, postural instability, and altered executive function, contribute to the high risk of falling in Parkinson's disease (PD). Dual-task training is an established method to reduce this risk. Motor-perceptual task combinations typically require a patient to walk while simultaneously engaging in a perceptual task. Motor-executive dual-tasking (DT) combines locomotion with executive function tasks. One augmented reality treadmill training (AR-TT) study revealed promising results of a perceptual dual-task training with a markedly reduced frequency of falls especially in patients with PD. We here propose to compare the effects of two types of concurrent tasks, perceptual and executive, on high-intensity TT). Patients will be trained with TT alone, in combination with an augmented reality perceptual DT (AR-TT) or with an executive DT (Random Number Generation; RNG-TT). The results are expected to inform research on therapeutic strategies for the training of balance in PD.
ET-Class: An Energy Transfer-Based Classification of Space Debris Removal Methods and Missions
Authors: Yalçın Barış Can , Martinez Carol , Hubert Delisle Maxime , Rodriguez Gonzalo , Zheng James , Olivares-Mendez Miguel
Published: March 2022

Space debris is positioned as a fatal problem for current and future space missions. Many effective space debris removal methods have been proposed in the past decade, and several techniques have been either tested on the ground or in parabolic flight experiments. Nevertheless, no uncooperative debris has been removed from any orbit until this moment. Therefore, to expand this research field and progressing the development of space debris removal technologies, this paper reviews and compares the existing technologies with past, present, and future methods and missions. Moreover, since one of the critical problems when designing space debris removal solutions is how to transfer the energy between the chaser/de-orbiting kit and target during the first interaction, this paper proposes a novel classification approach, named ET-Class (Energy Transfer Class). This classification approach provides an energy-based perspective to the space debris phenomenon by classifying how existing methods dissipate or store energy during the first contact.
RETRACTED: The Effect of Productive Safety Net Programme on Household Food Consumption and Dietary Diversity in Ethiopia
Authors: Feyisa Mebratu Negera
Published: March 2022

This study empirically investigates the effect of productive safety net programme (PSNP) on household food consumption and dietary diversity in Ethiopia. The study applied random effects with instrumental variable to estimate the effect of PSNP membership. The study indicates that though PSNP membership improves household food consumption, it reduces household dietary diversity score. Household food consumption and dietary diversity are also significantly influenced by sex, age, education status of household head, household size, livestock ownership, distance to the nearest market and participation in non-farm activities. The findings of this study suggest that PSNP membership should be reinforced by building household awareness of the benefits of consuming a variety of foods. In addition, PSNP membership should be designed to endow the households to accumulate essential assets, especially livestock.
Aggressive Angiomyxoma in the Scrotum: A Case Series and Literature Review
Authors: Sun Juan , Lian Peng H. , Ye Zi X. , Dong De X. , Ji Zhi G. , Wen Jin , Li Han Z.
Published: March 2022

Purpose

Aggressive angiomyxoma (AAM) was identified as a distinct clinicopathological entity in 1983. Since then, a few cases of its occurrence in the scrotum have been reported. This case series was performed to increase clinicians' understanding of the clinical features and treatment of AAM in the scrotum.

Methods

We evaluated the clinical presentations, treatments, and follow-up of two patients with AAM in the scrotum in our hospital and 34 cases reported in the literature.

Results

Among the 36 patients, the average age was 48.3 ± 20.6 years old (range from 1 to 81); the average maximum diameter of the tumor was 8.36 cm (1.6–25 cm); the site of one (2.78%) patient was located in the epididymis, two (5.56%) in the testes, five (13.89%) in the spermatic cord, and 28 (77.77%) in the scrotum. The clinical symptoms were generally non-specific and 20 patients inadvertently discovered their slow-growing painless masses. The treatments for all these patients were surgical excision once the tumor had been found and one case underwent excision followed by radiotherapy. The median follow-up time for the remaining 32 cases was 24.5 months (1 to 84 months). Recurrence occurred in three cases (9.09%) at the primary sites and no cases of distant metastasis.

Conclusion

AAM of the scrotum can occur in middle-aged and elderly men. The clinical manifestation generally involves a long history of asymptomatic masses or swelling in the scrotum. Ultrasound is the most commonly used diagnostic technique but magnetic resonance imaging may be more effective. The mainly treatment is surgical excision and postoperative histopathological examination is still the gold standard for its diagnosis. Although it is locally aggressive, metastasis is extremely rare in males.
SpyGlass DS-Directed Radiofrequency Ablation With Double Biliary Metal Stent Placement for Managing Recurrent Obstructive Jaundice Secondary to Castleman Disease: A Case Report of a Rare Disease
Authors: Chen Yongjin , Fu Chang , Chen Junhong , Pan Weicong , Fu Yu , Liu Kai
Published: March 2022

Castleman disease (CD) rarely presents with obstructive jaundice, which poses a diagnostic and therapeutic challenge to the management of the disease. A 40-year-old man was referred to our hospital for emergent management of upper abdominal pain. An abdominal mass was removed, and the postoperative pathology showed retroperitoneum CD, which was subsequently managed by adjuvant therapy of combination chemotherapy and steroids. One month later, a biliary metal stent was placed due to the presentation of obstructive jaundice. After ~3 months, the patient experienced another episode of obstructive jaundice, and SpyGlass DS cholangioscopy was performed via the biliary tract for biopsy, which pathologically showed biliary malignancies. Radiofrequency ablation was performed with a probe, and another uncovered metal stent was placed within the existing metal stent. No stent occlusion occurred during a 6-month follow-up period. In conclusion, CD rarely presents with obstructive jaundice, and a combination of radiofrequency ablation with metal stent implantation under cholangioscopy can prolong the stent patency time and the survival time of patients.
Resiliencia, bienestar y estrés postraumático ante el confinamiento y pandemia del COVID-19
Authors: Sánchez-Hernández Óscar , Canales Ana , Everaert Nathalie
Published: March 2022

La pandemia del COVID-19 y las medidas de distanciamiento social han supuesto un impacto negativo en la salud mental. El objetivo principal es estudiar el papel de la resiliencia como factor protector, ante el impacto de la pandemia y el confinamiento del COVID-19. La muestra fue de 179 participantes con una media de edad de 40,85 años (DE = 13,7). Los análisis indican que la resiliencia predice de forma muy significativa el bienestar psicológico y el estado de ánimo y es un factor protector ante el estrés postraumático. En menor medida para la facilidad de llevar la pandemia que puede estar mediado por otros factores. Los efectos de la pandemia y el confinamiento parecen haber elevado de forma muy significativa los síntomas de estrés postraumático. Las mujeres presentan más estrés postraumático y menos bienestar psicológico. El grupo de edad más afectado es el de los jóvenes (18-25 años).
Estilo Personal del Terapeuta en Psicólogos que trabajan en Discapacidad Intelectual
Authors: Casari Leandro , Rivas Ganem María Florencia , Richard Federico
Published: March 2022

El objetivo de este artículo es describir el estilo personal del terapeuta (EPT) en psicólogos clínicos que trabajan con pacientes con discapacidad intelectual, y analizar el efecto del enfoque teórico y de los años de experiencia profesional. Se trabajó con 100 psicoterapeutas de Argentina que divididos en dos grupos iguales: 50 que trabajan con personas con discapacidad intelectual y 50 que trabajan en consultorio privado. Como resultado se encontraron diferencias significativas en tres dimensiones del EPT: Operativa, Involucración, Expresiva, las cuales obtuvieron puntajes superiores en el primer grupo de terapeutas. Por otro lado, el enfoque teórico no mostró un efecto diferencial sobre los grupos de terapeutas excepto en la función Expresiva, y se encontró una diferencia significativa en los años de experiencia profesional en los terapeutas de discapacidad intelectual. Se discuten estos resultados con investigaciones previas y se reflexiona acerca de la importancia de estudiar el perfil de los terapeutas.
Historia de la Psicoterapia de Grupo: Antecedentes, Inicio y Desarrollo
Authors: Gil Escudero Guillermo , Rodríguez Zafra Mónica
Published: March 2022

Se describen, en primer lugar, los antecedentes de la psicoterapia de grupo haciendo referencia al momento y circunstancias del descubrimiento de la Terapia en grupo como modalidad terapéutica, así como al inicio del desarrollo de conceptos y técnicas grupales. En segundo lugar, se exponen los inicios de la psicoterapia psicodinámica de grupo, que se fundamentó en su inicio esencialmente en la aplicación de los conceptos psicoanalíticos al contexto grupal. Asimismo se examina la aportación a este campo psicoterapéutico del concepto de “grupo como totalidad” y su influencia en la práctica clínica. A continuación se analiza el periodo de expansión de la psicoterapia de grupo, que implicó el desarrollo de múltiples y muy diversas modalidades de psicoterapia de grupo y su aplicación a entornos y problemáticas muy diferentes. Por último, se exponen las características de la terapia de grupo integradora, que se caracteriza por la acumulación e integración de las diversas aportaciones, teóricas y técnicas, a esta modalidad de psicoterapia que se han realizado a lo largo del tiempo desde su inicio hasta la actualidad.
La Formación en Psicoterapia de Grupo. El necesario cambio de los programas asistenciales y de formación
Authors: Gómez Esteban Rosa
Published: March 2022

La Psicoterapia es una disciplina, no una técnica, y ha de ser el tratamiento de elección en todas las psicopatologías. La Psicoterapia de grupo será de elección en trastornos leves y moderados, y combinada con psicofármacos en trastornos graves. 

Los Programas de Formación de los futuros especialistas y los de Formación Continuada han de revisarse y actualizarse para incluir un “Área de capacitación en Psicoterapia”, en sus diversas modalidades, y poder cumplir con la cartera de servicios del Servicio Nacional de Salud (SNS) La capacitación en Psicoterapia ha de ser un objetivo prioritario en los Programas de Formación de los médicos y psicólogos residentes (MIR y PIR), y en la Formación Continuada. 

La formación en Psicoterapia ha de estar íntimamente ligada a la asistencia, a las prácticas en grupos terapéuticos y familiares en los servicios públicos. Han de revisarse los Programas de Psicoterapia Asistenciales para lograr las horas de acreditación, y lograr al final de la residencia el título de psicoterapeuta. Con voluntad política se pueden organizar las horas teóricas y prácticas a lo largo de los cuatro años de residencia.

Las metodologías grupales en la formación son muy eficaces para pensar la organización del equipo, de la asistencia, docencia e investigación en el campo de la Salud Mental.
Leg Configuration Analysis and Prototype Design of Biped Robot Based on Spring Mass Model
Authors: Che Junjie , Pan Yang , Yan Wei , Yu Jiexian
Published: March 2022

The leg structure with high dynamic stability can make the bionic biped robot have the inherent conditions to perform elastic and highly dynamic motion. Compared with the quadruped robot, the leg structure of the biped robot is more complex and has more degrees of freedom. This also complicates kinematic and dynamic modeling. In this paper, the kinematics model of a bionic biped robot is established. The leg configuration of the robot is a series parallel hybrid mechanism with five active joints and six passive joints. The mechanism is a spring mass model that interacts organically with the environment and mimics the characteristics of human walking well. By analyzing the topological configuration of leg mechanism, we use the screw theory to establish the forward and inverse kinematics models. Then, we build the prototype, and use a step gait to test the model and prototype. The research of this paper has obvious application significance for the design and iteration of biped robot prototype.
Applying Machine Learning Techniques to the Audit of Antimicrobial Prophylaxis
Authors: Shi Zhi-Yuan , Hon Jau-Shin , Cheng Chen-Yang , Chiang Hsiu-Tzy , Huang Hui-Mei
Published: March 2022

High rates of inappropriate use of surgical antimicrobial prophylaxis were reported in many countries. Auditing the prophylactic antimicrobial use in enormous medical records by manual review is labor-intensive and time-consuming. The purpose of this study is to develop accurate and efficient machine learning models for auditing appropriate surgical antimicrobial prophylaxis. The supervised machine learning classifiers (Auto-WEKA, multilayer perceptron, decision tree, SimpleLogistic, Bagging, and AdaBoost) were applied to an antimicrobial prophylaxis dataset, which contained 601 instances with 26 attributes. Multilayer perceptron, SimpleLogistic selected by Auto-WEKA, and decision tree algorithms had outstanding discrimination with weighted average AUC > 0.97. The Bagging and SMOTE algorithms could improve the predictive performance of decision tree against imbalanced datasets. Although with better performance measures, multilayer perceptron and Auto-WEKA took more execution time as compared with that of other algorithms. Multilayer perceptron, SimpleLogistic, and decision tree algorithms have outstanding performance measures for identifying the appropriateness of surgical prophylaxis. The efficient models developed by machine learning can be used to assist the antimicrobial stewardship team in the audit of surgical antimicrobial prophylaxis. In future research, we still have the challenges and opportunities of enriching our datasets with more useful clinical information to improve the performance of the algorithms.
Ultra-Dilute Gas of Polarons in a Bose–Einstein Condensate
Authors: Ardila Luis A. Peña
Published: March 2022

We investigate the properties of a dilute gas of impurities embedded in an ultracold gas of bosons that forms a Bose–Einstein condensate (BEC). This work focuses mainly on the equation of state (EoS) of the impurity gas at zero temperature and the induced interaction between impurities mediated by the host bath. We use perturbative field-theory approaches, such as Hugenholtz–Pines formalism, in the weakly interacting regime. In turn, for strong interactions, we aim at non-perturbative techniques such as quantum–Monte Carlo (QMC) methods. Our findings agree with experimental observations for an ultra dilute gas of impurities, modeled in the framework of the single impurity problem; however, as the density of impurities increases, systematic deviations are displayed with respect to the one-body Bose polaron problem.
Smartphone-Based Biosensor Devices for Healthcare: Technologies, Trends, and Adoption by End-Users
Authors: Madrid Rossana E. , Ashur Ramallo Fernando , Barraza Daniela E. , Chaile Roberto E.
Published: March 2022

Smart biosensors are becoming an important support for modern healthcare, even more so in the current context. Numerous smartphone-based biosensor developments were published in recent years, some highly effective and sensitive. However, when patents and patent applications related to smart biosensors for healthcare applications are analyzed, it is surprising to note that, after significant growth in the first half of the decade, the number of applications filed has decreased considerably in recent years. There can be many causes of this effect. In this review, we present the state of the art of different types of smartphone-based biosensors, considering their stages of development. In the second part, a critical analysis of the possible reasons why many technologies do not reach the market is presented. Both technical and end-user adoption limitations were addressed. It was observed that smart biosensors on the commercial stage are still scarce despite the great evolution that these technologies have experienced, which shows the need to strengthen the stages of transfer, application, and adoption of technologies by end-users.
Heat Stress Impairs Maternal Endometrial Integrity and Results in Embryo Implantation Failure by Regulating Transport-Related Gene Expression in Tongcheng Pigs
Authors: Lian Weisi , Gao Dengying , Huang Cheng , Zhong Qiqi , Hua Renwu , Lei Minggang
Published: March 2022

Heat stress (HS) poses a significant threat to production and survival in the global swine industry. However, the molecular regulatory effects of heat stress on maternal endometrial cells are poorly understood in pigs during early embryo implantation. In this study, we systematically examined morphological changes in the endometrium and the corresponding regulation mechanism in response to HS by combining scanning electron microscopy (SEM), hematoxylin/eosin (H&E) staining, western blot, and RNA-seq analyses. Our results showed that HS led to porcine endometrium damage and endometrial thinness during embryo implantation. The expression levels of cell adhesion-related proteins, including N-cadherin and E-cadherin, in the uterus were significantly lower in the heat stress group (39 ± 1 °C, n = 3) than in the control group (28 ± 1 °C, n = 3). A total of 338 up-regulated genes and 378 down-regulated genes were identified in porcine endometrium under HS. The down-regulated genes were found to be mainly enriched in the pathways related to the microtubule complex, immune system process, and metalloendopeptidase activity, whereas the up-regulated genes were mainly involved in calcium ion binding, the extracellular region, and molecular function regulation. S100A9 was found to be one of the most significant differentially expressed genes (DEGs) in the endometrium under HS, and this gene could promote proliferation of endometrial cells and inhibit their apoptosis. Meanwhile, HS caused endometrial epithelial cell (EEC) damage and inhibited its proliferation. Overall, our results demonstrated that HS induced uterine morphological change and tissue damage by regulating the expression of genes associated with calcium ions and amino acid transport. These findings may provide novel molecular insights into endometrial damage under HS during embryo implantation.
Immune Checkpoint Inhibitor Combination Therapy versus Sunitinib as First-Line Treatment for Favorable-IMDC-Risk Advanced Renal Cell Carcinoma Patients: A Meta-Analysis of Randomized Clinical Trials
Authors: Manneh Ray , Lema Mauricio , Carril-Ajuria Lucía , Ibatá Linda , Martínez Susan , Castellano Daniel , de Velasco Guillermo
Published: March 2022

Background: Novel combination therapies have been shown to improve the outcomes of treatment-naive patients with locally advanced or metastatic renal cell carcinoma (aRCC). However, the optimal systemic therapy for aRCC of favorable risk has yet to be clarified. We aimed to evaluate the efficacy and safety of different immunotherapy (IO) combinations, either with another IO (IO–IO) or with an antiangiogenic (IO–TKI), versus sunitinib in the first-line setting in aRCC patients with favorable IMDC risk. Methods: We conducted a systematic search for evidence in PubMed, Ovid MEDLINE, Embase, and the Cochrane Central Register of Controlled Trials published up to February 2021. The GRADE approach was used to assess the quality of evidence. Survival hazard ratios were extracted for analysis in the favorable-risk aRCC subgroup (IMDC). A sensitivity analysis was performed excluding trials of combination therapy without TKI. Results: Five randomized controlled phase III trials with a total of 1088 patients were included in the analysis. The studies compared different combinations versus sunitinib monotherapy. All clinical trials reported overall survival (OS), progression-free survival (PFS), and objective response rate (ORR) data. Four out of five trials reported complete response (CR). There was no difference in OS nor PFS between treatment arms in the IMDC favorable-risk subgroup analysis (OS: HR = 1.07, 95% CI = 0.81–1.41; PFS: HR = 0.74, 95% CI = 0.46–1.19). A benefit in ORR and CR was found for combination therapy vs. sunitinib (ORR: HR = 1.89, 95% CI = 1.29–2.76; CR: HR = 3.58, 95% CI = 2.04–6.28). In the sensitivity analysis, including only IO–TKI vs. sunitinib, no difference in OS was found; however, an advantage in PFS was observed (OS: HR = 0.99, 95% CI 0.69–1.43; PFS: HR = 0.60 (0.45–0.81). The safety profile reported is consistent with previous reports. We did not find differences in the incidence of any adverse event (AE) or of grade ≥3 AEs. Conclusion: This meta-analysis shows that combinations of IO–KI as first-line treatment in favorable-IMDC-risk aRCC improve PFS, ORR, and CR, but not OS, versus sunitinib.
Macrophage Infiltration Correlates with Genomic Instability in Classic Hodgkin Lymphoma
Authors: Hančić Suzana , Gršković Paula , Gašparov Slavko , Ostojić Kolonić Slobodanka , Dominis Mara , Korać Petra
Published: March 2022

Hodgkin lymphoma (HL) is a biologically diverse group of lymphoid tumors, which accounts for 1% of all de novo neoplasms in the world’s population. It is divided into two main groups: the more common classic Hodgkin lymphoma (cHL) and the less common nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL). cHL is further divided into four subtypes, which differ in morphology and the contents of tumor microenvironment. Macrophages are one of the components of tumor microenvironment known to contribute to creating an immunosuppressive microenvironment, which inhibits the activity of cells expressing granzyme B against tumor cells, even when tumor cells are infected with Epstein–Barr virus (EBV). Our research aimed to explore the association between the specific contents of tumor microenvironment and the genetic anomalies in tumor cells. The presence and the relative percentage of cytotoxic T lymphocytes and macrophages was detected by immunohistochemical staining of the antigens specific for certain cell populations. Fluorescent in situ hybridization was used to detect anomalies in the genome of tumor cells and in situ hybridization was used to detect the presence of EBV. Our results show an association between the number of CD163+ macrophages and the number of TP53 copies or BCL6 gene translocation. Patients who had a higher number of CD163+ macrophages infiltrating tumor tissue and three or higher number of copies of TP53 showed poorer survival. We conclude that the presence of macrophages may contribute to genetic instability in cHL, which drives the progression of cHL and decreases survival of the patients.
Retinitis Pigmentosa (RP): The Role of Oxidative Stress in the Degenerative Process Progression
Authors: Vingolo Enzo M. , Casillo Lorenzo , Contento Laura , Toja Francesca , Florido Antonio
Published: March 2022

Purpose: Retinitis Pigmentosa is a term that includes a group of inherited bilateral and progressive retinal degenerations, with the involvement of rod photoreceptors, which frequently leads to blindness; oxidative stress may be involved in the degeneration progression as proposed by several recent studies. The goal of this study is to evaluate whether circulating free radicals taken from capillary blood are related to one of the most important features of Retinitis pigmentosa that can affect frequently patients: cystoid macular oedema (CME). Materials: A total of 186 patients with Retinitis Pigmentosa (range: 25–69 years) were enrolled; all patients completed an ophthalmologic examination and SD-OCT at baseline and were divided into three subgroups according to the SD-OCT features. ROS blood levels were determined using FORT with monitoring of free oxygen radicals. Results: Test levels of free oxygen radicals were significantly increased, almost twice, in RP patients showing cystoid macular oedema and significantly increased compared to the control group. (p < 0.001). Discussion: Our findings suggest that oxidative stress may speed cone photoreceptors’ morphological damage (CMT); because long lasting oxidative stress in the RP may cause oxidative damage, with animal models of RP suggesting this is a micromolecular mechanism of photoreceptors’ (cone) death, it can be similar to cone damage in human RP eyes. The limitations of this paper are the relatively small sample, the horizontal design of the study, and the lack of data about the levels of ROS in the vitreous body.
Gene Profile of Adipose Tissue of Patients with Pheochromocytoma/Paraganglioma
Authors: Klímová Judita , Mráz Miloš , Kratochvílová Helena , Lacinová Zdeňka , Novák Květoslav , Michalský David , Kvasnička Jan , Holaj Robert , Haluzíková Denisa , Doležalová Radka Petráková , Zítek Matěj , Krátká Zuzana , Todorovová Veronika , Widimský Jiří , Haluzík Martin , Zelinka Tomáš , Petrák Ondřej
Published: March 2022

Background: Brown adipose tissue (BAT) is a therapeutic target to combat obesity and related disorders. Pheochromocytoma and functional paraganglioma (PPGL) are associated with activated BAT due to catecholamine excess. Our aim was to evaluate BAT activity by gene profile and assess its relation to clinical characteristics and overproduced catecholamine. Methods: mRNA expression of 15 genes in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) was measured via RT-PCR in 25 patients with PPGL and 14 controls undergoing cholecystectomy. Results: We found in VAT of PPGL higher expression of UCP1 (p < 0.001), CEBPB, PPARGC1A (both p < 0.001), PRDM16 (p = 0.069) and DIO2 (p = 0.005). UCP1 expression correlated only with norepinephrine levels and its metabolite. UCP1 expression, among others, correlated negatively with BMI, age and positively with HDLc levels. Dominance of BAT or BeAT markers was not assessed in PPGL. In SAT of PPGL, we found higher expression of ADRB3, CIDEA (both p < 0.05), and PPARGC1A (p = 0.001), but not UCP1. Conclusion: We demonstrate signs of UCP1-dependent norepinephrine-induced thermogenesis connected with higher expression of DIO2, PPARGC1A, CEBPB and PRDM16 in retroperitoneal VAT of PPGL and its relations to circulating HDLc and triglycerides levels. However, no direct relationship with increased basal energy metabolism measured by calorimetry was found.
Molecular Mechanisms Underlying the Retrieval and Extinction of Morphine Withdrawal-Associated Memories in the Basolateral Amygdala and Dentate Gyrus
Authors: Franco-García Aurelio , Fernández-Gómez Francisco José , Gómez-Murcia Victoria , Hidalgo Juana M. , Milanés M. Victoria , Núñez Cristina
Published: March 2022

Despite their indisputable efficacy for pain management, opiate prescriptions remain highly controversial partially due to their elevated addictive potential. Relapse in drug use is one of the principal problems for addiction treatment, with drug-associated memories being among its main triggers. Consequently, the extinction of these memories has been proposed as a useful therapeutic tool. Hence, by using the conditioned place aversion (CPA) paradigm in rats, we investigated some of the molecular mechanisms that occurr during the retrieval and extinction of morphine withdrawal memories in the basolateral amygdala (BLA) and the hippocampal dentate gyrus (DG), which control emotional and episodic memories, respectively. The retrieval of aversive memories associated with the abstinence syndrome paralleled with decreased mTOR activity and increased Arc and GluN1 expressions in the DG. Additionally, Arc mRNA levels in this nucleus very strongly correlated with the CPA score exhibited by the opiate-treated rats. On the other hand, despite the unaltered mTOR phosphorylation, Arc levels augmented in the BLA. After the extinction test, Arc and GluN1 expressions were raised in both the DG and BLA of the control and morphine-treated animals. Remarkably, Homer1 expression in both areas correlated almost perfectly with the extinction showed by morphine-dependent animals. Moreover, Arc expression in the DG correlated strongly with the extinction of the CPA manifested by the group treated with the opiate. Finally, our results support the coordinated activity of some of these neuroplastic proteins for the extinction of morphine withdrawal memories in a regional-dependent manner. Present data provide evidence of differential expression and activity of synaptic molecules during the retrieval and extinction of aversive memories of opiate withdrawal in the amygdalar and hippocampal regions that will likely permit the development of therapeutic strategies able to minimize relapses induced by morphine withdrawal-associated aversive memories.
Feasibility Study of a Network Meta-Analysis and Unanchored Population-Adjusted Indirect Treatment Comparison of Niraparib, Olaparib, and Bevacizumab as Maintenance Therapies in Patients with Newly Diagnosed Advanced Ovarian Cancer
Authors: Lorusso Domenica , Guy Holly , Samyshkin Yevgeniy , Hawkes Carol , Estenson Kasey , Coleman Robert L.
Published: March 2022

Selecting a first-line (1L) maintenance option for ovarian cancer is challenging given the variety of therapies, differing trials, and the lack of head-to-head data for angiogenesis and poly(ADP-ribose) polymerase (PARP) inhibitors. Thus, indirect treatment comparisons (ITCs) can aid treatment decision making. This study assessed the feasibility of two ITCs, a network meta-analysis (NMA) and a population-adjusted ITC (PAIC), comparing the efficacy of the PARP inhibitor niraparib in the PRIMA trial (NCT02655016) with other 1L maintenance treatments. A systematic literature review was conducted to identify trials using the Cochrane Handbook for Systematic Reviews of Interventions to assess differences in trial design, population characteristics, treatment arms, and outcome measures. All 12 trials identified were excluded from the NMA due to the absence of a common comparator and differences in survival measures and/or inclusion criteria. The PAIC comparing PRIMA and PAOLA-1 trials was also not feasible due to differences in inclusion criteria, survival measures, and the previous receipt of chemotherapy/bevacizumab. Neither ITC met recommended guidelines for analysis; the results of such comparisons would not be considered appropriate evidence when selecting 1L maintenance options in ovarian cancer. ITCs in this setting should be performed cautiously, as many factors can preclude objective trial comparisons.
Vesicular MicroRNA as Potential Biomarkers of Viral Rebound
Authors: Bazié Wilfried Wenceslas , Boucher Julien , Traoré Isidore Tiandiogo , Kania Dramane , Somé Diane Yirgnur , Alary Michel , Gilbert Caroline
Published: March 2022

Changes in the cellular microRNA (miRNA) expression profile in response to HIV infection, replication or latency have been reported. Nevertheless, little is known concerning the abundance of miRNA in extracellular vesicles (EVs). In the search for a reliable predictor of viral rebound, we quantified the amount of miR-29a, miR-146a, and miR-155 in two types of plasma extracellular vesicles. Venous blood was collected from 235 ART-treated and ART-naive persons living with HIV (85 with ongoing viral replication, ≥20 copies/mL) and 60 HIV-negative participants at five HIV testing or treatment centers in Burkina Faso. Large and small plasma EVs were purified and counted, and mature miRNA miR-29a, miR-146a, and miR-155 were measured by RT-qPCR. Diagnostic performance of miRNA levels in large and small EVs was evaluated by a receiver operating characteristic curve analysis. The median duration of HIV infection was 36 months (IQR 14–117). The median duration of ART was 34 months (IQR 13–85). The virus was undetectable in 63.8% of these persons. In the others, viral load ranged from 108 to 33,978 copies/mL (median = 30,032). Large EVs were more abundant in viremic participants than aviremic. All three miRNAs were significantly more abundant in small EVs in persons with detectable HIV RNA, and their expression levels in copies per vesicle were a more reliable indicator of viral replication in ART-treated patients with low viremia (20–1000 copies/mL). HIV replication increased the production of large EVs more than small EVs. Combined with viral load measurement, quantifying EV-associated miRNA abundance relative to the number of vesicles provides a more reliable marker of the viral status. The expression level as copies per small vesicle could predict the viral rebound in ART-treated patients with undetectable viral loads.