bluuebunny/crossref_metadata_2025 huggingface dataset:
Modelling End-User Behavior and Behavioral Change in Smart Grids. An Application of the Model of Frame Selection
Authors: Hoffmann Sebastian , Adelt Fabian , Weyer Johannes
Published: December 2020

This paper presents an agent-based model (ABM) for residential end-users, which is part of a larger, interdisciplinary co-simulation framework that helps to investigate the performance of future power distribution grids (i.e., smart grid scenarios). Different modes of governance (strong, soft and self-organization) as well as end-users’ heterogeneous behavior represent key influential factors. Feedback was implemented as a measure to foster grid-beneficial behavior, which encompasses a range of monetary and non-monetary incentives (e.g., via social comparison). The model of frame selection (MFS) serves as theoretical background for modelling end-users’ decision-making. Additionally, we conducted an online survey to ground the end-user sub-model on empirical data. Despite these empirical and theoretical foundations, the model presented should be viewed as a conceptual framework, which requires further data collection. Using an example scenario, representing a lowly populated residential area (167 households) with a high share of photovoltaic systems (30%), different modes of governance were compared with regard to their suitability for improving system stability (measured in cumulated load). Both soft and strong control were able to decrease overall fluctuations as well as the mean cumulated load (by approx. 10%, based on weekly observation). However, we argue that soft control could be sufficient and more societally desirable.
Exploring the Impact of a TPSR Program on Transference of Responsibility Goals within a Preschool Setting: An Action Research Study
Authors: Santos Fernando , Miguel Jacinta , Wright Paul M. , Sá Cesar , Saraiva Linda
Published: December 2020

The teaching personal and social responsibility (TPSR) model has been extensively used in a vast array of settings. However, few TPSR studies have focused on preschool settings. The purpose of this action research study was to analyze the experiences of a program leader, her preschool children, and their parents throughout a TPSR program focused on transference of responsibility model goals. The participants were 25 preschool children, six parents, and a program leader involved in a preschool setting located in the north of Portugal. Data were collected through reflexive journaling, participant observations, semi-structured interviews, and focus group interviews. Findings suggest the TPSR model could be a useful instructional model for preschool teachers focused on providing social and emotional learning opportunities to their students. In order to foster transference, parents played a pivotal role in this process and were included in the intervention, which appeared to enhance life skill transfer.
Prospective Interpersonal and Intrapersonal Predictors of Initiation and Cessation of Non-Suicidal Self-Injury among Chinese Adolescents
Authors: Wang Hui , Wang Quanquan , Liu Xia , Gao Yemiao , Chen Zixun
Published: December 2020

(1) Purpose: Non-suicidal self-injury (NSSI) possibly emerges as well as remits in adolescence. To explore the development and transition of NSSI, this study examined the association between a wide range of interpersonal and intrapersonal predictors of NSSI initiation and cessation. (2) Methods: Chinese adolescents (N = 913) completed self-reported surveys at baseline and at a six-month follow-up. The sample included 625 adolescents who reported no NSSI and 288 adolescents who reported engagement in NSSI at baseline. (3) Results: Among the adolescents without NSSI at baseline, 24.3% engaged in NSSI at follow-up (NSSI initiation group). Among the adolescents with NSSI at baseline, 33.3% reported no NSSI at follow-up (NSSI cessation group). Loneliness, beliefs about adversity, problem behavior, and prosocial behavior were the significant factors in predicting subsequent NSSI initiation. None of the potential predicting factors were associated with subsequent NSSI cessation. (4) Conclusions: These results indicate the importance of intrapersonal factors in Chinese culture, which could be used to identify at-risk adolescents and to design interventions.
Body Composition in International Sprint Swimmers: Are There Any Relations with Performance?
Authors: Dopsaj Milivoj , Zuoziene Ilona Judita , Milić Radoje , Cherepov Evgeni , Erlikh Vadim , Masiulis Nerijus , di Nino Andrea , Vodičar Janez
Published: December 2020

The paper addresses relations between the characteristics of body composition in international sprint swimmers and sprint performance. The research included 82 swimmers of international level (N = 46 male and N = 36 female athletes) from 8 countries. We measured body composition using multifrequency bioelectrical impedance methods with “InBody 720” device. In the case of male swimmers, it was established that the most important statistically significant correlation with sprint performance is seen in variables, which define the quantitative relationship between their fat and muscle with the contractile potential of the body (Protein-Fat Index, r = 0.392, p = 0.007; Index of Body Composition, r = 0.392, p = 0.007; Percent of Skeletal Muscle Mass, r = 0.392, p = 0.016). In the case of female athletes, statistically significant relations with sprint performance were established for variables that define the absolute and relative amount of a contractile component in the body, but also with the variables that define the structure of body fat characteristics (Percent of Skeletal Muscle Mass, r = 0.732, p = 0.000; Free Fat Mass, r = 0.702, p = 0.000; Fat Mass Index, r = −0.642, p = 0.000; Percent of Body Fat, r = −0.621, p = 0.000). Using Multiple Regression Analysis, we managed to predict swimming performance of sprint swimmers with the help of body composition variables, where the models defined explained 35.1 and 75.1% of the mutual variability of performance, for male and female swimmers, respectively. This data clearly demonstrate the importance of body composition control in sprint swimmers as a valuable method for monitoring the efficiency of body adaptation to training process in order to optimize competitive performance.
Does Flourishing Reduce Engagement in Unhealthy and Risky Lifestyle Behaviours in Emerging Adults?
Authors: Sofija Ernesta , Harris Neil , Phung Dung , Sav Adem , Sebar Bernadette
Published: December 2020

Emerging adulthood is a transitional life stage with increased probability of risky and unhealthy lifestyle behaviours that are known to have strong links with premature mortality and morbidity. Wellbeing, as a positive subjective experience, is identified as a factor that encourages self-care and may steer individuals away from risky lifestyle behaviours. Investigating wellbeing–behaviour links in the emerging adult population may increase understanding of the factors that lead to, and ways to prevent, engagement in risky behaviours. This study examines the association between flourishing, that is, the experience of both high hedonic and eudaimonic wellbeing, and a broad range of risky and unhealthy lifestyle behaviours among emerging adults in Australia. A cross-sectional survey of 1155 emerging adults aged 18–25 years measured wellbeing, socio-demographics, and six groups of lifestyle behaviours surrounding substance use, physical activity, diet, sex, sun protection, and driving. Bivariate and multivariate statistics were used to analyse the data. The findings revealed that flourishing was negatively associated with more dangerous types of risk behaviours, such as driving under the influence of drugs, and positively associated with self-care behaviours, such as healthier dietary behaviour and sun protection. If enabling emerging adults to flourish can contribute to reduced engagement in risky/unhealthy lifestyle behaviours, then promoting it is an important goal for health promotion efforts not only because flourishing is desirable in its own right, but also to bring about sustainable change in behaviour. Further research is needed to inform the designs of such interventions.
Plasma Inflammatory Biomarkers Associated with Advanced Liver Fibrosis in HIV–HCV-Coinfected Individuals
Authors: Chen Xiaochen , Liu Xing , Duan Song , Tang Renhai , Zhou Sujuan , Ye Runhua , Yang Yuecheng , Wang Jibao , Yao Shitang , He Na
Published: December 2020

Background: HIV and HCV coinfection leads to accelerated liver fibrosis, in which microbial translocation and systemic inflammation might play important roles. Objective: This study aimed to provide an extensive profile of the plasma microbial translocation and inflammation biomarkers associated with advanced liver fibrosis among HIV–HCV-coinfected patients. Methods: This cross-sectional study recruited 343 HIV–HCV-coinfected patients on combination antiretroviral therapy (cART) from a rural prefecture of Yunnan province in Southwest China. The plasma concentrations of sCD14 and 27 cytokines and chemokines were assayed and compared against advanced or mild levels of liver fibrosis. Results: Of the 343 HIV–HCV-coinfected patients, 188 (54.8%) had severe or advanced liver fibrosis (FIB-4 > 3.25). The patients with advanced liver fibrosis (FIB-4 > 3.25 vs. FIB-4 ≤ 3.25) had higher plasma levels of interleukin (IL)-1β, IL-6, IL-7, IL-9, IL-12, IL-15, IL-17, granulocyte macrophage colony stimulating factor (GM-CSF), Interferon-γ (IFN-γ), tumor necrosis factor (TNF-α), IL-4, IL-10, IL-13, fibroblast growth factor 2 (FGF-basic), and Monocyte chemoattractant protein-1 (MCP-1). Multivariable logistic regression models showed that advanced liver fibrosis was associated with an increased plasma level of IL-1β, IL-6, IL-7, IL-12, IL-17, GM-CSF, IFN-γ, IL-4, IL-10, MCP-1, Eotaxin, and FGF-basic, with FGF-basic continuing to be positively and significantly associated with advanced liver fibrosis, after Bonferroni correction for multiple comparisons (adjusted odds ratio (aOR) = 1.92; 95%CI: 1.32–2.81; p = 0.001). Plasma sCD14 was also significantly associated with advanced liver fibrosis (aOR = 1.13; 95%CI: 1.01–1.30; p = 0.049). Conclusions: HIV–HCV-coinfected patients are living with a high prevalence of advanced liver fibrosis which coexists with a mixture of elevated plasma inflammation and microbial translocation biomarkers. The significant associations of advanced liver fibrosis with FGF-basic and sCD14 may reveal pathogenic mechanisms and potential clinical intervention targets for liver fibrosis in HCV–HIV coinfection.
Tissue Engineering of Cartilage Using a Random Positioning Machine
Authors: Wehland Markus , Steinwerth Paul , Aleshcheva Ganna , Sahana Jayashree , Hemmersbach Ruth , Lützenberg Ronald , Kopp Sascha , Infanger Manfred , Grimm Daniela
Published: December 2020

Articular cartilage is a skeletal tissue of avascular nature and limited self-repair capacity. Cartilage-degenerative diseases, such as osteoarthritis (OA), are difficult to treat and often necessitate joint replacement surgery. Cartilage is a tough but flexible material and relatively easy to damage. It is, therefore, of high interest to develop methods allowing chondrocytes to recolonize, to rebuild the cartilage and to restore joint functionality. Here we studied the in vitro production of cartilage-like tissue using human articular chondrocytes exposed to the Random Positioning Machine (RPM), a device to simulate certain aspects of microgravity on Earth. To screen early adoption reactions of chondrocytes exposed to the RPM, we performed quantitative real-time PCR analyses after 24 h on chondrocytes cultured in DMEM/F-12. A significant up-regulation in the gene expression of IL6, RUNX2, RUNX3, SPP1, SOX6, SOX9, and MMP13 was detected, while the levels of IL8, ACAN, PRG4, ITGB1, TGFB1, COL1A1, COL2A1, COL10A1, SOD3, SOX5, MMP1, and MMP2 mRNAs remained unchanged. The STRING (Search Tool for the Retrieval of Interacting Genes/Proteins) analysis demonstrated among others the importance of these differentially regulated genes for cartilage formation. Chondrocytes grown in DMEM/F-12 medium produced three-dimensional (3D) spheroids after five days without the addition of scaffolds. On day 28, the produced tissue constructs reached up to 2 mm in diameter. Using specific chondrocyte growth medium, similar results were achieved within 14 days. Spheroids from both types of culture media showed the typical cartilage morphology with aggrecan positivity. Intermediate filaments form clusters under RPM conditions as detected by vimentin staining after 7 d and 14 d. Larger meshes appear in the network in 28-day samples. Furthermore, they were able to form a confluent chondrocyte monolayer after being transferred back into cell culture flasks in 1 g conditions showing their suitability for transplantation into joints. Our results demonstrate that the cultivation medium has a direct influence on the velocity of tissue formation and tissue composition. The spheroids show properties that make them interesting candidates for cellular cartilage regeneration approaches in trauma and OA therapy.
Anti-Inflammatory Activity of Extracts and Pure Compounds Derived from Plants via Modulation of Signaling Pathways, Especially PI3K/AKT in Macrophages
Authors: Merecz-Sadowska Anna , Sitarek Przemysław , Śliwiński Tomasz , Zajdel Radosław
Published: December 2020

The plant kingdom is a source of important therapeutic agents. Therefore, in this review, we focus on natural compounds that exhibit efficient anti-inflammatory activity via modulation signaling transduction pathways in macrophage cells. Both extracts and pure chemicals from different species and parts of plants such as leaves, roots, flowers, barks, rhizomes, and seeds rich in secondary metabolites from various groups such as terpenes or polyphenols were included. Selected extracts and phytochemicals control macrophages biology via modulation signaling molecules including NF-κB, MAPKs, AP-1, STAT1, STAT6, IRF-4, IRF-5, PPARγ, KLF4 and especially PI3K/AKT. Macrophages are important immune effector cells that take part in antigen presentation, phagocytosis, and immunomodulation. The M1 and M2 phenotypes are related to the production of pro- and anti-inflammatory agents, respectively. The successful resolution of inflammation mediated by M2, or failed resolution mediated by M1, may lead to tissue repair or chronic inflammation. Chronic inflammation is strictly related to several disorders. Thus, compounds of plant origin targeting inflammatory response may constitute promising therapeutic strategies.
Efficiency of Recombinant CRISPR/rCas9-Mediated miRNA Gene Editing in Rice
Authors: Chung Pil Joong , Chung Hoyong , Oh Nuri , Choi Joohee , Bang Seung Woon , Jung Se Eun , Jung Harin , Shim Jae Sung , Kim Ju-Kon
Published: December 2020

Drought is one of the major environmental stresses adversely affecting crop productivity worldwide. Precise characterization of genes involved in drought response is necessary to develop new crop varieties with enhanced drought tolerance. Previously, we identified 66 drought-induced miRNAs in rice plants. For the further functional investigation of the miRNAs, we applied recombinant codon-optimized Cas9 (rCas9) for rice with single-guide RNAs specifically targeting mature miRNA sequences or sites required for the biogenesis of mature miRNA. A total of 458 T0 transgenic plants were analyzed to determine the frequency and type of mutations induced by CRISPR/rCas9 on 13 independent target miRNAs. The average mutation frequency for 13 genes targeted by single guide RNAs (sgRNAs) in T0 generation was 59.4%, including mono-allelic (8.54%), bi-allelic (11.1%), and hetero-allelic combination (39.7%) mutations. The mutation frequency showed a positive correlation with Tm temperature of sgRNAs. For base insertion, one base insertion (99%) was predominantly detected in transgenic plants. Similarly, one base deletion accounted for the highest percentage, but there was also a significant percentage of cases in which more than one base was deleted. The deletion of more than two bases in OsmiR171f and OsmiR818b significantly reduced the level of corresponding mature miRNAs. Further functional analysis using CRISPR/Cas9-mediated mutagenesis confirmed that OsmiR818b is involved in drought response in rice plants. Overall, this study suggests that the CRISPR/rCas9 system is a powerful tool for loss-of-function analysis of miRNA in rice.
The Impact of the ‘Mis-Peptidome’ on HLA Class I-Mediated Diseases: Contribution of ERAP1 and ERAP2 and Effects on the Immune Response
Authors: Tedeschi Valentina , Paldino Giorgia , Paladini Fabiana , Mattorre Benedetta , Tuosto Loretta , Sorrentino Rosa , Fiorillo Maria Teresa
Published: December 2020

The strong association with the Major Histocompatibility Complex (MHC) class I genes represents a shared trait for a group of autoimmune/autoinflammatory disorders having in common immunopathogenetic basis as well as clinical features. Accordingly, the main risk factors for Ankylosing Spondylitis (AS), prototype of the Spondyloarthropathies (SpA), the Behçet’s disease (BD), the Psoriasis (Ps) and the Birdshot Chorioretinopathy (BSCR) are HLA-B*27, HLA-B*51, HLA-C*06:02 and HLA-A*29:02, respectively. Despite the strength of the association, the HLA pathogenetic role in these diseases is far from being thoroughly understood. Furthermore, Genome-Wide Association Studies (GWAS) have highlighted other important susceptibility factors such as Endoplasmic Reticulum Aminopeptidase (ERAP) 1 and, less frequently, ERAP2 that refine the peptidome presented by HLA class I molecules to CD8+ T cells. Mass spectrometry analysis provided considerable knowledge of HLA-B*27, HLA-B*51, HLA-C*06:02 and HLA-A*29:02 immunopeptidome. However, the combined effect of several ERAP1 and ERAP2 allelic variants could generate an altered pool of peptides accounting for the “mis-immunopeptidome” that ranges from suboptimal to pathogenetic/harmful peptides able to induce non-canonical or autoreactive CD8+ T responses, activation of NK cells and/or garbling the classical functions of the HLA class I molecules. This review will focus on this class of epitopes as possible elicitors of atypical/harmful immune responses which can contribute to the pathogenesis of chronic inflammatory diseases.
Vasa Vasorum Lumen Narrowing in Brain Vascular Hyalinosis in Systemic Hypertension Patients Who Died of Ischemic Stroke
Authors: Gychka Sergiy G. , Shults Nataliia V. , Nikolaienko Sofia I. , Marcocci Lucia , Sariipek Nurefsan E. , Rybka Vladyslava , Malysheva Tatiana A. , Dibrova Vyacheslav A. , Suzuki Yuichiro J. , Gavrish Alexander S.
Published: December 2020

Ischemic stroke is a major cause of death among patients with systemic hypertension. The narrowing of the lumen of the brain vasculature contributes to the increased incidence of stroke. While hyalinosis represents the major pathological lesions contributing to vascular lumen narrowing and stroke, the pathogenic mechanism of brain vascular hyalinosis has not been well characterized. Thus, the present study examined the postmortem brain vasculature of human patients who died of ischemic stroke due to systemic hypertension. Hematoxylin and eosin staining and immunohistochemistry showed the occurrence of brain vascular hyalinosis with infiltrated plasma proteins along with the narrowing of the vasa vasorum and oxidative stress. Transmission electron microscopy revealed endothelial cell bulge protrusion into the vasa vasorum lumen and the occurrence of endocytosis in the vasa vasorum endothelium. The treatment of cultured microvascular endothelial cells with adrenaline also promoted the formation of the bulge as well as endocytic vesicles. The siRNA knockdown of sortin nexin-9 (a mediator of clathrin-mediated endocytosis) inhibited adrenaline-induced endothelial cell bulge formation. Adrenaline promoted protein-protein interactions between sortin nexin-9 and neural Wiskott–Aldrich syndrome protein (a regulator of actin polymerization). Spontaneously hypertensive stroke-prone rats also exhibited lesions indicative of brain vascular hyalinosis, the endothelial cell protrusion into the lumen of the vasa vasorum, and endocytosis in vasa vasorum endothelial cells. We propose that endocytosis-dependent endothelial cell bulge protrusion narrows the vasa vasorum, resulting in ischemic oxidative damage to cerebral vessels, the formation of hyalinosis, the occurrence of ischemic stroke, and death in systemic hypertension patients.
Novel Liver X Receptor Ligand GAC0001E5 Disrupts Glutamine Metabolism and Induces Oxidative Stress in Pancreatic Cancer Cells
Authors: Srivastava Shivangi , Widmann Scott , Ho Charles , Nguyen Donovan , Nguyen Alexis , Premaratne Asitha , Gustafsson Jan-Åke , Lin Chin-Yo
Published: December 2020

Pancreatic ductal adenocarcinoma (PDAC) is the predominant form of pancreatic cancer with a high mortality rate due to the lack of early detection and effective treatment options for advanced diseases. Metabolic reprogramming, a common hallmark of malignant transformation in pancreatic cancer, is critical for the growth and survival of cancer cells and a potential target mechanism for the treatment of pancreatic cancer. PDAC cells have upregulated glutamine metabolism to meet their biosynthetic and oxidative demands. Liver X receptors (LXRs) are ligand-dependent transcription factors involved in maintaining metabolic homeostasis. LXRs regulate critical cancer-related processes and pathways, including cholesterol, glucose and lipid metabolism, and inflammatory and immune responses. Analysis of transcriptomic data from PDAC clinical samples reveals overexpression of LXRs and their target genes in tumors as compared to normal tissue controls. Targeting LXRs with the novel LXR inverse agonist and degrader GAC0001E5 inhibited PDAC cell proliferation. Using a metabolomics approach, we discovered that 1E5 inhibits glutamine anaplerosis and induces oxidative stress, which are detrimental to PDAC cells. These findings highlight a novel role for LXR in regulating cancer metabolism and the potential application of LXR modulators in targeting cancer metabolism in pancreatic cancer and other malignancies.
Antibodies Isolated from Rheumatoid Arthritis Patients against Lysine-Containing Proteus mirabilis O3 (S1959) Lipopolysaccharide May React with Collagen Type I
Authors: Durlik-Popińska Katarzyna , Żarnowiec Paulina , Lechowicz Łukasz , Gawęda Józef , Kaca Wiesław
Published: December 2020

Most rheumatic diseases, including rheumatoid arthritis (RA), are characterized by immune disorders that affect antibody activity. In the present study, using Dot blot and ELISA assay, we showed that patients with rheumatic disease produced significantly more antibodies against lipopolysaccharide (LPS) P. mirabilis O3 compared to healthy donors (p < 0.05), and affinity purified antibodies against LPS O3 may cross-react with collagen type I. It was demonstrated that purified of antibodies isolated from RA patients sera, reacted stronger with the collagen than healthy donors (p = 0.015), and cross-reaction was correlated with level of anti-citrullinated peptide antibodies (r = 0.7, p = 0.003). Moreover, using six different lipopolysaccharides were demonstrated the significant correlations in sera reactivity among lysine-containing lipopolysaccharides observed in patients’ sera (p < 0.05). Using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR) it was shown that unique wavenumbers of sera spectra correlate with reactivity with lipopolysaccharides allowing distinguish patients from healthy blood donors. Antibodies adsorption by synthetic antigens shows that in patients’ group anti-LPS O3 antibodies can be adsorbed by both amides of galacturonic acid and lysine or threonine, which suggests less specificity of antibodies binding with non-carbohydrate LPS component. The observed correlations suggest that non-carbohydrate components of LPS may be an important epitope for less specific anti-LPS antibodies, which might lead to cross-reactions and affect disease development.
A Different Look at Polynomials within Man-Machine Interaction
Authors: Bajorska-Harapińska Beata , Pleszczyński Mariusz , Różański Michał , Smoleń-Duda Barbara , Smuda  Adrian , Wituła  Roman
Published: December 2020

Undoubtedly, one of the most powerful applications that allow symbolic computations is Wolfram Mathematica. However, it turns out that sometimes Mathematica does not give the desired result despite its continuous improvement. Moreover, these gaps are not filled by many authors of books and tutorials. For example, our attempts to obtain a compact symbolic description of the roots of polynomials or coefficients of a polynomial with known roots using Mathematica have often failed and they still fail. Years of our work with theory, computations, and different kinds of applications in the area of polynomials indicate that an application ‘offering’ the user alternative methods of solving a given problem would be extremely useful. Such an application would be valuable not only for people who look for solutions to very specific problems but also for people who need different descriptions of solutions to known problems than those given by classical methods. Therefore, we propose the development of an application that would be not only a program doing calculations but also containing an interactive database about polynomials. In this paper, we present examples of methods and information which could be included in the described project.
Biotic Threats to Cycas micronesica Continue to Expand to Complicate Conservation Decisions
Authors: Deloso Benjamin E. , Terry L. Irene , Yudin Lee S. , Marler Thomas E.
Published: December 2020

Invasions of non-native species can threaten native biodiversity, and island ecosystems are ideal for studying these phenomena. In this article, first, we report on the invasive species that combine to threaten the island cycad Cycas micronesica by reviewing the history of previously reported invasions and providing an update of recent invasions. Then, we prioritize the threat status of each herbivore and the interactions among them. Plant damage was initiated in 2003─2005 by the non-native Aulacaspis yasumatsui Takagi armored scale, Erechthias sp. Meyrick leaf miner, and Luthrodes pandava Horsfield butterfly, which elicited unprecedented irruptions of the native Acalolepta marianarum Aurivillius stem borer and increased herbivory by feral pigs (Sus scrofa L.). The combined impact of these five consumers represents the greatest sustained threat to the cycad tree species. Mitigation of the damage caused by phytophagous non-native species is urgently needed to conserve this unique gymnosperm tree.
Seasonal Migrations of Pantala flavescens (Odonata: Libellulidae) in Middle Asia and Understanding of the Migration Model in the Afro-Asian Region Using Stable Isotopes of Hydrogen
Authors: Borisov Sergey N. , Iakovlev Ivan K. , Borisov Alexey S. , Ganin Mikhail Yu. , Tiunov Alexei V.
Published: December 2020

In Middle Asia, the dragonfly Pantala flavescens makes regular seasonal migrations. In spring, sexually mature dragonflies (immigrants) arrive in this region for reproduction. Dragonflies of the aboriginal generation (residents) develop in about two months, and migrate south in autumn. Residents of Middle Asia have significantly lower δ2H values (−123.5 (SD 17.2)‰, n = 53) than immigrants (−64.4 (9.7)‰, n = 12), as well as aboriginal dragonfly species from Ethiopia (−47.9 (10.8)‰, n = 4) and the Sahel zone (−50.1 (15.5)‰, n = 11). Phenological data on P. flavescens in the Afro-Asian region and a comparison with published isotopic data on migratory insects from this region suggest that (i) the probable area of origin of P. flavescens immigrants is located in tropical parts of East Africa and/or the Arabian Peninsula and (ii) the autumn migration of Middle Asian residents to the south may also pass through the Indian Ocean. We assume that in the Afro-Asian region, there is an extensive migration circle of P. flavescens covering East Africa, Central Asia and the Indian subcontinent with a total length of more than 14,000 km.
Statistical Prediction of Extreme Storm Surges Based on a Fully Supervised Weather-Type Downscaling Model
Authors: Costa Wagner , Idier Déborah , Rohmer Jérémy , Menendez Melisa , Camus Paula
Published: December 2020

Increasing our capacity to predict extreme storm surges is one of the key issues in terms of coastal flood risk prevention and adaptation. Dynamically forecasting storm surges is computationally expensive. Here, we focus on an alternative data-driven approach and set up a weather-type statistical downscaling for daily maximum storm surge (SS) prediction, using atmospheric hindcasts (CFSR and CFSv2) and 15 years of tidal gauge station measurements. We focus on predicting the storm surge at La Rochelle–La Pallice tidal gauge station. First, based on a sensitivity analysis to the various parameters of the weather-type approach, we find that the model configuration providing the best performance in SS prediction relies on a fully supervised classification using minimum daily sea level pressure (SLP) and maximum SLP gradient, with 1° resolution in the northeast Atlantic domain as the predictor. Second, we compare the resulting optimal model with the inverse barometer approach and other statistical models (multi-linear regression; semi-supervised and unsupervised weather-types based approaches). The optimal configuration provides more accurate predictions for extreme storm surges, but also the capacity to identify unusual atmospheric storm patterns that can lead to extreme storm surges, as the Xynthia storm for instance (a decrease in the maximum absolute error of 50%).
Monitoring and Surveillance of Aerial Mycobiota of Rice Paddy through DNA Metabarcoding and qPCR
Authors: Franco Ortega Sara , Ferrocino Ilario , Adams Ian , Silvestri Simone , Spadaro Davide , Gullino Maria Lodovica , Boonham Neil
Published: December 2020

The airborne mycobiota has been understudied in comparison with the mycobiota present in other agricultural environments. Traditional, culture-based methods allow the study of a small fraction of the organisms present in the atmosphere, thus missing important information. In this study, the aerial mycobiota in a rice paddy has been examined during the cropping season (from June to September 2016) using qPCRs for two important rice pathogens (Pyricularia oryzae and Bipolaris oryzae) and by using DNA metabarcoding of the fungal ITS region. The metabarcoding results demonstrated a higher alpha diversity (Shannon–Wiener diversity index H′ and total number of observed species) at the beginning of the trial (June), suggesting a higher level of community complexity, compared with the end of the season. The main taxa identified by HTS analysis showed a shift in their relative abundance that drove the cluster separation as a function of time and temperature. The most abundant OTUs corresponded to genera such as Cladosporium, Alternaria, Myrothecium, or Pyricularia. Changes in the mycobiota composition were clearly dependent on the average air temperature with a potential impact on disease development in rice. In parallel, oligotyping analysis was performed to obtain a sub-OTU identification which revealed the presence of several oligotypes of Pyricularia and Bipolaris with relative abundance changing during monitoring.
Ejidos, Urbanization, and the Production of Inequality in Formerly Agricultural Lands, Guadalajara, Mexico, 1975–2020
Authors: Barleta Leonardo , Carrillo Mateo , Frank Zephyr , Steiner Erik
Published: December 2020

The ejido is an institution of communal land tenure and governance administered by the Mexican government. This paper assesses the current visual appearance of landscapes and implicit land use in ejidal lands on the periphery of Guadalajara, Mexico, using Google Street View (GSV) images tagged for signs of urban distress. Distressed landscapes are associated with the temporal process of urban expansion—newer settlements tend to be more visibly impoverished. Concentrations of vulnerable housing are correlated with encroached-upon ejidal lands in a process that was underway by the 1970s, well before Mexico’s neoliberal turn. Ejidos on the urban periphery, created to support agricultural communities during Mexico’s radical period of agrarian reform, are now sites of urban sprawl and impoverishment. Nevertheless, these communities remain legally salient as federal entities with respect to the disposition of land. Their presence complicates the historical evolution of land use in the urban periphery in ways that do not fit into classical central place models. We conclude that the presence of ejidos is associated with rapid and chaotic urbanization by migrants and the loss of agricultural capacity in Guadalajara’s periphery.
Low-Cycle Fatigue Behavior of the Novel Steel and 30SiMn2MoV Steel at 700 °C
Authors: Zhao Chao , Zhang Jin , Fu Jiawei , Lian Yong , Zhang Zunjun , Zhang Cheng , Huang Jinfeng
Published: December 2020

As a newly developed gun barrel steel, the novel steel has shown excellent high-temperature strength, high resistance to wear and erosion, contributing to the superior ballistic life of gun barrels. As ballistic life increases, the fatigue life becomes essential for the safety and reliability of gun barrels. This paper presents a comparison of the low cycle fatigue (LCF) behaviors between a novel steel and 30SiMn2MoV steel at 700 °C. A strain-controlled fatigue test was carried out on the novel steel and 30SiMn2MoV steel in the strain range from 0.2 to 0.6%. The cyclic stress response behaviors of the novel steel and 30SiMn2MoV steel show cyclic softening behavior. In addition, the shape of the hysteresis rings of the novel steel and 30SiMn2MoV steel exhibit no-Masing model behavior. Energy–life relationships results show that the novel steel has higher fatigue resistance than the 30SiMn2MoV steel at 700 °C. The results of fatigue fracture analysis show that the failure mode of the 30SiMn2MoV steel is a mixed mode of intergranular fracture and transgranular fracture, while the failure mode of the novel steel is intergranular fracture. The cyclic softening of the two materials can be attributed to the lath structure with a high density of dislocations gradually transforms into low energy subcrystalline and cellular structures at 700 °C. The novel steel has a better fatigue life than the 30SiMn2MoV steel at 700 °C and different strain amplitudes, which is mainly related to the carbides and lath martensite in the materials.
Analysis of the Fire Behavior of Polymers (PP, PA 6 and PE-LD) and Their Improvement Using Various Flame Retardants
Authors: Hohenwarter Dieter , Mattausch Hannelore , Fischer Christopher , Berger Matthias , Haar Bernd
Published: December 2020

The fire behavior of polymers is examined primarily with the time-dependent heat release rate (HRR) measured with a cone calorimeter. The HRR is used to examine the fire behavior of materials with and without flame retardants, especially Polypropylene (PP-Copo) and Polyethylene (PE-LD). Polypropylene is stored for up to 99 days under normal conditions and the heat release rate shows especially changes about 100 s after irradiation with cone calorimeter, which may be caused by aging effects. The effect of crosslinking to the burning behavior of PP was examined too. Polyamides (PA 6) are irradiated with a radiation intensity of 25 kW/m2 to 95 kW/m2 and fire-related principles between radiation intensity and time to ignition can be derived from the measurement results. In order to comprehensively investigate the fire behavior of PP (also with flame retardant additives), the samples were also exposed to a flame, according to UL 94 with small power (50 W) and is inflamed with the power of a few 100 W. The irradiation causes different trigger mechanisms for the flame retardant additives in a plastic than the flame exposure. It is shown that the compound, which is favorable for irradiation, is not necessarily good for flame exposure. It can be seen that expandable graphite alone or with the addition of other additives is a very effective flame retardant for PP.
Effect of Co, Ti and Cr Additions on Microstructure, Magnetic Properties and Corrosion Resistance of Magnetocaloric Gd-Ge-Si Alloys
Authors: Hasiak Mariusz , Chęcmanowski Jacek G. , Kucharska Barbara , Łaszcz Amadeusz , Kolano-Burian Aleksandra , Kaleta Jerzy
Published: December 2020

The paper presents studies of microstructure, magnetic and corrosion properties of the Gd58Ge20Si22, Gd56Ge20Si22Co2, Gd56Ge20Si22Ti2 and Gd56Ge20Si22Cr2 (at.%) alloys after isothermal heat treatment at 1450 K for 2 h. The structure investigations of the produced materials performed by X-ray diffraction show the presence of Gd5Ge2Si2-type phase in all investigated samples. DC and AC magnetic measurements confirmed that the Curie temperature depends on the chemical composition of the produced alloys. From M(T) characteristics, it was found that the lowest Curie point (TC = 268 K) was estimated for the Gd58Ge20Si22 sample, whereas the highest value of the Curie temperature (TC = 308 K) was for the Gd56Ge20Si22Cr2 alloys. Moreover, the GdGeSi alloy without alloying additions shows the highest magnetic entropy change |ΔSM| = 15.07 J⋅kg−1⋅K−1 for the maximum magnetic field of 2 T. The maximum |ΔSM| measured for the Gd56Ge20Si22 with the addition of Co, Ti or Cr for the same magnetic field was obtained in the vicinity of the Curie point and equals to 2.92, 2.73 and 2.95 J⋅kg−1⋅K−1, respectively. Electrochemical studies of the produced materials for 60 min and 55 days exposure in 3% NaCl solution show that the highest stability and corrosion resistance were exhibited the sample with added of Ti.
Electronic Structure and Surface Properties of Copper Thiocyanate: A Promising Hole Transport Material for Organic Photovoltaic Cells
Authors: Odeke Bonaventure A. , Chung Gyang D. , Fajemisin Jesutofunmi A. , Suraj Kabir S. , Tonui Denis K. , Tobi Ayinla R. , Bewaale Thomas C. , Ajibola Jamiu A. , Dzade Nelson Y.
Published: December 2020

Considering the significance of hexagonal copper thiocyanate (β-CuSCN) in several optoelectronic technologies and applications, it is essential to investigate its electronic structure and surface properties. Herein, we have employed density functional theory (DFT) calculations to characterise the band structure, density of states, and the energy-dependent X-ray photoelectron (XPS) valence band spectra at variable excitation energies of β-CuSCN. The surface properties in the absence and presence of dimethyl sulfoxide (DMSO), a solvent additive for improving perovskite solar cells’ power conversion efficiency, have also been systematically characterised. β-CuSCN is shown to be an indirect band gap material (Eg = 3.68 eV) with the valence band edge demonstrated to change from being dominated by Cu-3d at soft X-ray ionisation photon energies to Cu-3p at hard X-ray ionisation photon energies. The adsorption energy of dimethyl sulfoxide (DMSO) on the (100) and (110) β-CuSCN surfaces is calculated at −1.12 and −0.91 eV, respectively. The presence of DMSO on the surface is shown to have a stabilisation effect, lowering the surface energy and tuning the work function of the β-CuSCN surfaces, which is desirable for organic solar cells to achieve high power conversion efficiencies.
Mechanical and Thermal Influences on Microstructural and Mechanical Properties during Process-Integrated Thermomechanically Controlled Forging of Tempering Steel AISI 4140
Authors: Behrens Bernd-Arno , Brunotte Kai , Petersen Tom , Diefenbach Julian
Published: December 2020

Thermomechanical treatment (TMT) describes the effect of thermal and mechanical conditions on the microstructure of materials during processing and offers possible integration in the forging process. TMT materials exhibit a fine-grained microstructure, leading to excellent mechanical properties. In this study, a two-step TMT upsetting process with intermediate cooling is used to demonstrate possibilities for a process-integrated treatment and corresponding properties. A water–air-based cooling system was designed to adjust different phase configurations by varying the target temperature and cooling rate. Four different thermal processing routes and four combinations of applied plastic strains are investigated in standardized mechanical tests and metallographic analyses. The applied TMT results in a finely structured bainitic microstructure of the investigated tempering steel AISI 4140 (42CrMo4) with different characteristics depending on the forming conditions. It can be shown that the demands of the standard (DIN EN ISO 683) in a quenched and tempered state can be fulfilled by means of appropriate forming conditions. The yield strength can be enhanced up to 1174 MPa while elongation at break is about 12.6% and absorbed impact energy reaches 58.5 J without additional heat treatment when the material is formed after rapid cooling.
Improved Stability and Activity of a Marine Peptide-N6NH2 against Edwardsiella tarda and Its Preliminary Application in Fish
Authors: Han Huihui , Li Ting , Wang Zhenlong , Teng Da , Mao Ruoyu , Hao Ya , Yang Na , Wang Xiumin , Wang Jianhua
Published: December 2020

Edwardsiella tarda can cause fatal gastro-/extraintestinal diseases in fish and humans. Overuse of antibiotics has led to antibiotic resistance and contamination in the environment, which highlights the need to find new antimicrobial agents. In this study, the marine peptide-N6 was amidated at its C-terminus to generate N6NH2. The antibacterial activity of N6 and N6NH2 against E. tarda was evaluated in vitro and in vivo; their stability, toxicity and mode of action were also determined. Minimal inhibitory concentrations (MICs) of N6 and N6NH2 against E. tarda were 1.29–3.2 μM. Both N6 and N6NH2 killed bacteria by destroying the cell membrane of E. tarda and binding to lipopolysaccharide (LPS) and genomic DNA. In contrast with N6, N6NH2 improved the stability toward trypsin, reduced hemolysis (by 0.19% at a concentration of 256 μg/mL) and enhanced the ability to penetrate the bacterial outer and inner membrane. In the model of fish peritonitis caused by E. tarda, superior to norfloxacin, N6NH2 improved the survival rate of fish, reduced the bacterial load on the organs, alleviated the organ injury and regulated the immunity of the liver and kidney. These data suggest that the marine peptide N6NH2 may be a candidate for novel antimicrobial agents against E. tarda infections.
Salmonella enterica in Invasive Lizard from Fernando de Noronha Archipelago: Serotyping, Antimicrobial Resistance and Molecular Epidemiology
Authors: Abrahão Carlos R. , Moreno Luisa Z. , Silva Jean C. R. , Benites Nilson R. , Matajira Carlos E. C. , Ferreira Fernando , Moreno Andrea M. , Dias Ricardo A.
Published: December 2020

Salmonella infection can pose serious health issues, especially to children, elders or immunosuppressed humans. Wild populations of reptiles can reach Salmonella prevalence of up to 100% and the direct or indirect transmission from reptiles to humans have been extensively reported. Fernando de Noronha (FN) is an inhabited oceanic archipelago in the northeast coast of Brazil, with an economy based on tourism. The tegu (Salvator merianae) is the largest lizard native to South America and was introduced to the archipelago in the early 20th century. This study determines the prevalence, serotypes, antimicrobial resistance, and molecular epidemiology of Salmonella enterica in the tegu population from FN archipelago. Results show that S. enterica is widely distributed in the FN tegu population, with 43.8% prevalence. The bacteria were isolated from 70.5% of the sampled sites and a total of 15 serotypes were detected in 98 S. enterica isolates. Strains were further classified into 31 genotypes. Recaptured animals presented distinct genotypes in each season, demonstrating a seasonal strain turnover. Most S. enterica isolates from FN tegus presented low antimicrobial resistance. This is possibly due to geographical isolation of the island population, hampering contact with strains from livestock from the continent, where antimicrobial resistance is common.
Multifunctional Amyloids in the Biology of Gram-Positive Bacteria
Authors: Álvarez-Mena Ana , Cámara-Almirón Jesús , de Vicente Antonio , Romero Diego
Published: December 2020

Since they were discovered, amyloids have proven to be versatile proteins able to participate in a variety of cellular functions across all kingdoms of life. This multitask trait seems to reside in their ability to coexist as monomers, aggregates or fibrillar entities, with morphological and biochemical peculiarities. It is precisely this common molecular behaviour that allows amyloids to cross react with one another, triggering heterologous aggregation. In bacteria, many of these functional amyloids are devoted to the assembly of biofilms by organizing the matrix scaffold that keeps cells together. However, consistent with their notion of multifunctional proteins, functional amyloids participate in other biological roles within the same organisms, and emerging unprecedented functions are being discovered. In this review, we focus on functional amyloids reported in gram-positive bacteria, which are diverse in their assembly mechanisms and remarkably specific in their biological functions that they perform. Finally, we consider cross-seeding between functional amyloids as an emerging theme in interspecies interactions that contributes to the diversification of bacterial biology.
High-Toughness Poly(lactic Acid)/Starch Blends Prepared through Reactive Blending Plasticization and Compatibilization
Authors: Hu Huan , Xu Ang , Zhang Dianfeng , Zhou Weiyi , Peng Shaoxian , Zhao Xipo
Published: December 2020

In this study, poly(lactic acid) (PLA)/starch blends were prepared through reactive melt blending by using PLA and starch as raw materials and vegetable oil polyols, polyethylene glycol (PEG), and citric acid (CA) as additives. The effects of CA and PEG on the toughness of PLA/starch blends were analyzed using a mechanical performance test, scanning electron microscope analysis, differential scanning calorimetry, Fourier-transform infrared spectroscopy, X-ray diffraction, rheological analysis, and hydrophilicity test. Results showed that the elongation at break and impact strength of the PLA/premixed starch (PSt)/PEG/CA blend were 140.51% and 3.56 kJ·m−2, which were 13.4 and 1.8 times higher than those of pure PLA, respectively. The essence of the improvement in the toughness of the PLA/PSt/PEG/CA blend was the esterification reaction among CA, PEG, and starch. During the melt-blending process, the CA with abundant carboxyl groups reacted in the amorphous region of the starch. The shape and crystal form of the starch did not change, but the surface activity of the starch improved and consequently increased the adhesion between starch and PLA. As a plasticizer for PLA and starch, PEG effectively enhanced the mobility of the molecular chains. After PEG was dispersed, it participated in the esterification reaction of CA and starch at the interface and formed a branched/crosslinked copolymer that was embedded in the interface of PLA and starch. This copolymer further improved the compatibility of the PLA/starch blends. PEGs with small molecules and CA were used as compatibilizers to reduce the effect on PLA biodegradability. The esterification reaction on the starch surface improved the compatibilization and toughness of the PLA/starch blend materials and broadens their application prospects in the fields of medicine and high-fill packaging.