bluuebunny/crossref_metadata_2025 huggingface dataset:
JQ1 Synergize with Anti-CD47 Antibody to Enhance the Function of Macrophages and Repress the Progression of Burkitt Lymphoma
Authors: Wang Shunan , Guo Wei , Zhao Yangzhi , Li Jia , Wan Xin , Liu Zhihe , Song Wantong , Bai Ou
Published: November 2021

Background: M2 macrophages, as the most prominent immune cells in Burkitt lymphoma (BL), are promising targets and anti-CD47 antibody could enhance the phagocytosis but be unable to eradicate tumor cells. JQ1, a C-MYC inhibitor, may enhance the function of macrophages and be a choice for combination therapy. Methods: The effect JQ1, on the expression of CD47 was measured. Then the synergy of JQ1 and anti-CD47 antibody was measured using phagocytosis assays. The effect of JQ1 on the polarization of macrophages was also detected. Finally, the efficiency and safety of JQ1 and anti-CD47 antibody combination therapy was explored in a groin orthotopically implanted Raji tumor model. Results: JQ1 could suppress the expression of CD47 on the surface of BL cells, thus synergize with anti-CD47 antibody to enhance the phagocytosis of macrophages. JQ1 could polarize macrophages from M2 to M1 while inhibiting the proliferation, inducing the apoptosis and blocking the cell cycle of BL cells. Finally, JQ1 and anti-CD47 antibody combination therapy could repress the progression of BL in NOD/SCID mice. Conclusions: Macrophages may be a promising target in BL and JQ1 combined with anti-CD47 antibody may be a potential therapeutic choice, providing theoretical basis for the use of this new targeted immunotherapy in clinical practice.
Application of Failure Detection Methods to Detect Information Attacks on the Control System
Authors: Radda Iureva , Alexey Margun
Published: November 2021

The paper examines the influence of information attacks on the dynamics of automatic control systems. Comparison of abnormal dynamics of control objects during attacks and device failures is carried out. The similarity of the consequences of information attacks and failures of the control system is analyzed, a method for identifying attacks based on the methods developed for detecting failures is developed. Computer modeling of the influence of information attacks and failures on the control system of a DC motor has been carried out. The simulation results allow making a conclusion about the applicability of the failure detection algorithms for detecting attacks.
Has-miR-875-5p Inhibits Tumorigenesis By Targeting USF2 Via TGF-β Signaling Pathway in Gastric Cancer
Authors: Gao Shenshuo , Zhang Zhikai , Wang Xubin , Ma Yan , Li Chensheng , Liu Hongjun , Jing Changqing , Li Leping , Guo Xiaobo
Published: November 2021

Background: Gastric cancer (GC) is one of the most common malignancies, and more and more evdiences show that the pathogenesis is regulated by various miRNAs. In this study, we investigated the role of miR-875 in GC. Methods: The expression of miR-875-5p was detected in human GC specimens and cell lines by miRNA RT-PCR. The effect of miR-875-5p on GC proliferation was determined by CCK-8 proliferation assay and EDU assay. Migration and invasion were examined by transwell migration and invasion assay and wound healing assay. The interaction between miR-875-5p and its target gene USF2 was verified by a dual luciferase reporter assay. The effects of miR-875-5p in vivo were studied in xenograft nude mice models. Related proteins were detected by Western blot. Results: The results showed that miR-875-5p inhibited the proliferation, migration and invasion of gastric cancer cells in vitro, and inhibited tumorigenesis in vivo. USF2 proved to be a direct target of miR-875-5p. Knockdown of USF2 partially counteracts the effects of miR-875-5p inhibitors. Overexpression of miR-875-5p can inhibit proliferation, migration, and invasion through the TGF-β signaling pathway by down-regulation of USF2 in GC, providing a new research direction for the diagnosis and targeted therapy of GC.Conclusions: MiR-875-5p can inhibited the progression of GC by directly targeting USF2 and negatively regulating TGF-β signaling pathway. In the future, miR-875-5p is expected to be used as a potential therapeutic target for GC therapy.
Shallow Temperature Lapse Rate Signified Elevation Dependent Warming in Different Treeline Environments in the Himalaya- Possible Implications to Treeline Vegetation
Authors: Joshi Rajesh , Tamang Ninchhen Dolma , Singh Surendra Pratap
Published: November 2021

There are emergent evidences that the rise in temperature in high altitude regions in comparison to low altitude of the Himalaya is more rapid than other parts of the World. This Elevation-dependent warming (EDW) can accelerate the rate of change in mountain ecosystems, including cryosphere, hydrology, biodiversity and socio-economic systems. In this paper, we present Temperature Lapse Rates (TLRs) from 20 stations for three treeline transects representing different climate regimes along the Himalayan arc. TLRs were calculated based on high temporal resolution data collected for two year (2017-18) from complex mountain terrain of treeline environment. The annual mean TLR increased with decreasing moisture, being markedly high at dry WH transect (-0.66℃/100 m) and lowest (-0.50℃/100 m) for moist EH transect. The One-Way ANOVA confirms that the TLR varied spatially, declining from West to East across the Himalayan arc, and significantly differ among seasons (F=3.2175; P = 0.03). The lowest mean TLRs were found during the winter season (EH: -0.46℃/100m; CH: -0.40℃/100m; WH: -0.31℃/100m). The monthly TLR varied within a narrow range (-0.49℃/100m to -0.54℃/100m) at EH transect, -0.24℃/100m to -0.68℃/100m at CH transect and from -0.26℃ to -0.90℃ at WH transect with lowest monthly TLR in December (-0.24 to -0.32℃/ 100m) for all three sites. Study shows moisture, snow albedo and reflectance play a key role as controlling factors on TLR in treeline environments. Higher growing season temperatures observed for treelines in Himalaya (8.4±1.8℃, 10.3±1.4℃, and 7.5±2.7℃) shows warmer treeline in Himalaya. The EDW may impact the dynamics of treeline, snow and moisture regime, surface energy balance, increased water stress, species distribution, and growing season of alpine vegetation in the Himalaya. The findings of the study could provide useful insight (ground-based) to re-parameterize the climate models over the Himalayan region. This study can facilitate improving interpolation of air temperature for ecological modeling studies in ungauged and the data-sparse regions, especially for the higher Himalaya where ground based station data are extremely scarce.
Extrapolating potential crop damage by insect pests based on land use data: examining inter-regional generality in agricultural landscapes
Authors: Tabuchi Ken , Takahashi Akihiko , Uesugi Ryuji , Okudera Shigeru , Yoshimura Hideto
Published: November 2021

ContextInter-regional relationships between landscape factors and biological responses under natural conditions are important but difficult to predict because of the differences in each landscape context and local environment. ObjectivesTo examine the inter-regional variability in relation to landscape factors and the biological response of an insect pest, we extrapolated a damage prediction model (‘the original model’) for rice using land-use data. We hypothesized that the original model would be applicable to new regions, but the predictive accuracy would be reduced. We predicted that adjusting the parameter coefficients would improve model performance (‘the adjusted model’). MethodsA field experiment was conducted in two regions that had a similar landscape context with the original region, in different years for each region, for four years in total. The proportion of rice damage and surrounding land-use within a 300-m radius was investigated. ResultsWhen ‘the original model’ was assigned to combined data from the original and extrapolated regions, the relationship between observed and predicted values was statistically significant, suggesting that there was an inter-regional common relationship. The relationship was not statistically significant if the model was applied only to the new regions. The adjusted model improved by 14% compared with the original model.ConclusionsThese results imply that in this pest–crop system, a common inter-regional biological response to arthropods is likely because of landscape factors, although local environmental factors must be considered. Application of such relationships is needed to identify or prevent pest hazards by offering region-wide management options.
Validation of Robotic-assisted Ureteroplasty with Buccal Mucosa Graft for Stricture at the Proximal and Middle Ureters: The First Comparative Study
Authors: Yang Che Hsueh , Lin Yi Sheng , Weng Wei Chun , Lu Chin Heng , Hsu Chao Yu , Tung Min Che , Ou Yen Chuan
Published: November 2021

Although ureteroplasty with buccal mucosa graft for long-segmental ureteral stenosis has been developed long ago, evidence was still restricted to case series in published literature. This study aims to validate ureteroplasty with buccal mucosa graft (BMG) in long-segment stricture at the proximal and middle ureters under designed comparative methods. From April 2015 to January 2019, we performed robotic-assisted ureteroplasty with BMG with a two-phase design and compared ureteroplasty and BMG (phase 2 surgery) with endoscopic stenting (phase 1 surgery). Paired data of effective renal plasma flow (ERPF), glomerular filtration rate (GFR), hydronephrosis grade, and physical and psychological domains of the World Health Organization Quality of Life (WHOQOL)-BREF were compared. A total of 29 patients were enrolled, and only three (10%) patients had hydronephrosis resolution after treatment with endoscopic stenting (p = 0.250 to baseline). Compared to endoscopic ureteral stent, Hedges’ g of ureteroplasty with BMG was 0.56 (95% CI: 0.43-0.69), 0.63 (95% CI: 0.46-0.80), 0.80 (95% CI: 0.56-1.04), and 1.06 (95% CI: 0.69-1.43) in EGFR, GFR, physical domain of WHOQOL-BREF, and psychological domain of WHOQOL-BREF, respectively (All significance; p<0.001). After 12-month follow-ups, no recurrence of stricture was reported. In conclusion, Robotic-assisted ureteroplasty with BMG onlay is efficient in reconstruction of long-segment stricture of the proximal and middle ureters.
Application of Synthetic Data to Establish the Working Framework for Multivariate Statistical Analysis of River Pollution Traceability - The Heavy Metals in Nankan River, Taiwan
Authors: Lin Chun-Chun , Lo Shang-Lien , Liou Sofia Ya-Hsuan
Published: November 2021

This study applied multivariate statistical analysis (MSA) to the synthetic data simulated by the river water quality model to investigate how two pollution sources with different characteristics and contributions affect the results of MSA. The results showed that when assessing the number and possible locations of pollution sources based on the results of cluster analysis (CA), hydrological information about surface water should be obtained to improve the accuracy of the results; when applying principal component analysis (PCA), the results of the second principal component (PC2) and the Pearson correlation coefficients among the pollutants should both be included, which can add more information about the characteristics of pollutant sources. In addition, this study found that the solid and liquid partition coefficients (Kd) of pollutants can affect the interpretation of the PCA results, so the Kd values should be determined before tracing the pollution sources to facilitate the evaluation of the source characteristics and potential targets. This study established a working framework for surface water pollution traceability to enhance the effectiveness of pollution traceability.
Intuitionistic Fuzzy Rough TOPSIS Method for Robot Selection using Einstein operators
Authors: Qadir Abbas , Naeem Muhammad , Abdullah Saleem , Ghanmi Nejib
Published: November 2021

Rough set and intuitionistic fuzzy set are very vital role in the decision making method for handling the uncertain and imprecise data of decision makers. The technique for order preference by similarity to ideal solution (TOPSIS) is very attractive method for solving the ranking and multi-criteria decision making (MCDM) problem. The primary goal of this paper is to introduce the Extended TOPSIS for industrial robot selection under intuitionistic fuzzy rough (IFR) information, where the weights of both, decision makers (DMs) and criteria are not-known. First, we develop Intuitionistic fuzzy rough (IFR) aggregation operators based on Einstein T-norm and T-conom, For this firstly we give the idea of intuitionistic fuzzy rough Einstein weighted averaging (IFREWA), intuitionistic fuzzy rough Einstein hybrid averaging (IFREHA) and intuitionistic fuzzy rough ordered weighted averaging (IFREOWA) aggregation operators. The fundamental properties of the proposed operators are described in detail. Furthermore to determine the unknown weights, a generalized distance measure are defined for IFRSs based on intuitionistic fuzzy rough entropy measure. Following that, the intuitionistic fuzzy rough information-based decision-making technique for multi-criteria group decision making (MCGDM) is developed, with all computing steps depicted in simplest form. For considering the conflicting attributes, our proposed model is more accurate and effective. Finally, an example of efficient industrial robot selection is presented to illustrate the feasibility of the proposed intuitionistic fuzzy rough decision support approaches, as well as a discussion of comparative outcomes, demonstrating that the results are feasible and reliable.
Negative Emotional Status and Influencing Factors of Young Employees in Center of Disease Control and Prevention
Authors: Han Lu , Li Qiyu , Zhang Yu , Liu Tuo , Ran Niu , Wang Qi , Zhao Lina
Published: November 2021

Objective To investigate the status and influencing factors of negative emotions among young employees in center of disease control and prevention. Methods Using the cluster sampling methods, 6099 employees aged 40 or below in center of disease control and prevention(CDC) of 32 province of China were interviewed by online questionnaire survey. The emotional conditions of anxiety and depression, and their influencing factors were analyzed. Results A total of 5,353 valid questionnaires were collected with the recovery rate of 87.77%. 2,871 cases of young employees had different degrees of negative emotions at work, accounting for about 53.60%. Regression analysis showed that gender, professional title, educational level, job satisfaction, chronic diseases, daily sleep duration, average weekly overtime, physical activity time, and sugary beverage intake were the influencing factors of negative emotions(P<0.05). Male, primary and below, never working overtime and daily physical activity time more than 30 minutes were protective factors for negative emotions(OR vale were 0.79, 0.68, 0.39 and 0.63, respectively, P<0.05). Bachelor degree or above, poor job satisfaction, chronic disease, daily sleep duration less than eight hours and drinking one to three sugary drinks a week were the risk factors for negative emotion(OR vale were1.21, 4.32, 2.16, 2.75 and 1.20, respectively, P<0.05). Conclusion Due to the influence of work pressure, lifestyle, chronic diseases and other factors, young employees in CDC have a certain degree of negative emotions at work, which should be paid enough attention. Meanwhile, corresponding measures should be taken according to the influencing factors to reduce the occurrence of negative emotions.
Effect of miR-192-5p on Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Idiopathic Scoliosis by Regulating Wnt/β-catenin Signaling Pathway via RSPO1
Authors: Yang Yifan , Xu Jing , Su Qingxin , Wu Yiran , Li Qizheng , Ma zongren , ding tao
Published: November 2021

BackgroundIdiopathic scoliosis (IS) is the most common structural scoliosis, which seriously affects not only patient’s physical and mental health but also quality of patient’s life. Abnormal osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is one of the causes of IS. However, the regulation mechanism of osteogenic differentiation of BMSCs in patients with IS remains to be further studied.MethodsSerum samples of 135 patients with IS were collected, and the expression of miRNA were detected by RT-qPCR. BMSCs from patients with IS were collected and the expression of miR-192-5p in BMSCs from IS patients and normal BMSCs was detected by RT-qPCR. Double luciferase reporter genes assay was used to verify the targeting relationship between miR-192-5p and RSPO1. The levels of RSPO1, osteogenic related proteins (OC, OPN and RUNX2) and Wnt/β-catenin signaling pathway related proteins (WNT3A and β-catenin) were detected by Western blotting. Alkaline phosphatase staining and alizarin red staining were used to evaluate the osteogenesis of BMSCs.ResultsmiR-192-5p was significantly up-regulated in serum and BMSCs of patients with IS. Alkaline phosphatase staining and alizarin red staining showed that miR-192-5p inhibitor promoted the osteogenic differentiation of BMSCs from IS patients. miR-192-5p targeted down-regulated the expression of RSPO1 in BMSCs from IS patients. In addition, overexpression of RSPO1 activated Wnt/β-catenin signaling pathway in BMSCs from IS patients. Furthermore, miR-192-5p/RSPO1 axis regulated levels of osteogenic related proteins (OC, OPN and RUNX2) in BMSCs from IS patients through Wnt/β-catenin signaling pathway, and affected the osteogenic differentiation of BMSCs.Conclusion﻿miR-192-5p, which was highly expressed in patients with IS, inhibited Wnt/β-catenin signaling pathway by down-regulating RSPO1 protein and then reduced the osteogenic differentiation ability of BMSCs.
Gender Differences in Prognosis after Primary Resection for Retroperitoneal Liposarcoma
Authors: Weng Cheng-Xin , Qi Yu-Han , Zhao Ji-Chun , Yuan Ding , Yang Yi , Wang Tie-Hao , Wang Jia-Rong , Huang Bin
Published: November 2021

Background: Current evidence regarding gender difference in retroperitoneal liposarcoma (RLPS) is scarce, we sought to investigate whether gender may affect prognosis after primary resection of RLPS.Methods: We used the Surveillance, Epidemiology, and End Results (SEER) database to identify RLPS patients from January 1973 to December 2015. Multivariate cox proportional hazards analysis was adopted to generate adjusted hazard ratio (AHR) and 95% confidence intervals (CI) of survival outcomes.Results: In total, 2108 RLPS patients, including 971 women and 1137 men, were identified, with a median follow-up of 45.0 (17.0-92.0) months. The 5-year and 10-year overall survival rates were 50.5% and 31.5% for men, and 60.4% and 42.5% for women. The 5-year and 10-year disease-specific survival rates for men and women were 71.5%, 57.3% and 76.3%, 62.1%, respectively. We found men were associated with an increased risk of all-cause mortality (AHR 1.3, 95%CI 1.0-1.6, P=.017) but not disease-specific mortality (AHR 1.2, 95%CI 0.9-1.6, P=.246). The subgroup analyses revealed that men were associated with an increased risk of all-cause mortality in patients with low-grade tumors (AHR 1.8, 95%CI 1.3-2.5) or patients received non-radical resection (AHR 1.6, 95%CI 1.2-2.1). Besides, in the subgroup of low-grade tumors, men were also associated with an increased risk of disease-specific mortality (AHR 2.0, 95%CI 1.2-3.3).Conclusion: Men may have worse survival after primary resection of RLPS compared with women, especially in patients with low-grade tumors or patients received non-radical resection. Gender-based disparities may deserve more attention in patients with RLPS.
Identification of a Novel Homozygous Nonsense Mutation in a Fetus with Bardet-Biedl Syndrome
Authors: Cai Meiying , Lin Min , Wang Xinrui , Su Linjuan , Wu Xiaoqing , Xie Xiaorui , Fu Xianguo , Lin Na , Xu Liangpu , Huang Hailong
Published: November 2021

Background: Bardet-Biedl syndrome (BBS) is a rare autosomal recessive genetic disorder with clinical and genetic heterogeneity. BBS is more commonly reported in adults and children than in fetuses. Here, we reported the intrauterine phenotype and molecular characterizations of a fetus with BBS. Methods: Chromosome karyotype analysis, whole exome sequencing (WES), and a single nucleotide polymorphism array (SNP-array) were used to analyze the genetic etiology of a fetus with enlarged kidneys, enhanced echo, and oligohydramnios. Results: The results of chromosome karyotype analysis and SNP-array on the fetus were normal. WES, however, revealed homozygous mutation of c.1177C>T (p.Arg393*) on exon 12 of the BBS1 gene, and heterozygous variation of c.2704G>A (p.Asp902Asn) on exon 22 of the CC2D2A gene. According to ACMG guidelines, c.1177C> T was identified as a pathogenic mutation and c.2704G>A was identified as an uncertain significance. Sanger sequencing showed that there was heterozygous mutation of c.1177C>T and heterozygous variation of c.2704G>A in the parents of the fetus. Conclusions: WES identified a novel homozygous nonsense mutation c.1177C>T in the BBS1 gene of a Chinese fetus. The finding provides more insight into BBS1 mutations in Asian populations in general, and provides a basis for genetic counseling.
Comparison of Simultaneous Single-position Oblique Lumbar Interbody Fusion and Percutaneous Pedicle Screw Fixation with Posterior Lumbar Interbody Fusion using O-arm Navigated C-arm free Technique for Lumbar Degenerative Diseases
Authors: Tan Ying , Tanaka Masato , Sonawane Sumeet , Uotani Koji , Oda Yoshiaki , Fujiwara Yoshihiro , Arataki Shinya , Yamauchi Taro , Takigawa Tomoyuki , Ito Yasuo
Published: November 2021

Background: To compare the clinical, surgical and radiographic outcomes of O-arm navigated C-arm free, simultaneous single-position oblique lumbar interbody fusion (OLIF) and percutaneous pedicle screw (PPS) fixation with minimally invasive posterior/ transforaminal lumbar interbody fusion (MI-PLIF/TLIF).Methods: This is retrospective comparative study. The study included 98 patients, 63 in single position OLIF (group SO) and 35 in MI-PLIF/TLIF (group P/T). Surgical time, blood loss, mobilization time after surgery and complications were analyzed for all patients. Clinical evaluation included visual analog scale (VAS) for back pain, oswestry disability index (ODI). Radiological parameters included cage height (CH), cage to disc ratio (CDR), DH change, pre and postoperative disc height (DH), foraminal height (FH), foraminal area (FA), segmental lordosis (SL). Results: In group SO (vs group P/T), surgical time, blood loss and mobilization time were 117.7± 34.1 minutes (171.8 ± 40.6 minutes, p<0.000001), 139.2 ± 82.0 ml (vs. 374.2 ± 247.7 ml, p<0.000001) and 2.7 ±1 .0 days (vs 3.9 ± 2.4 days, p<0.000001) respectively. The CH, CDR, DH change and postoperative DH, FH, FA increase were statistically significant in group SO compared to group P/T. VAS and ODI improvement were similar in both groups. Mobilization time is shorter in group SO. Total complication rate in group SO was 7% while that in group P/T was 11%. Conclusions: Simultaneous single position O-arm navigated C-arm free OLIF reduces the surgical time, blood loss, mobilization time after operation without the risk of an adverse event of intraoperative radiation to operating staff. Good indirect decompression can be achieved with this method. Clinical results were similar in both groups.
Lipidomics and body fat composition analysis characterises specific differences in cholesterol metabolism and steatosis between hepatitis C virus genotypes 1 and 3
Authors: Sheridan David A , Shawa Isaac Thom , Thomas E. Louise , Felmlee Daniel J. , Bridge Simon H. , Neely Dermot , Cobbold Jeremy F. , Holmes Elaine , Bassendine Margaret F. , Taylor-Robinson Simon D.
Published: November 2021

Background

Lipids play essential roles in the hepatitis C virus (HCV) life cycle and patients with chronic HCV infection display disordered lipid metabolism which resolves following successful anti-viral therapy. It has been proposed that HCV genotype 3 (HCV-G3) infection is an independent risk factor for hepatocellular carcinoma and evidence suggests lipogenic proteins are involved in hepatocarcinogenesis.
Aims

We aimed to characterise variation in host lipid metabolism between participants chronically infected with HCV genotype 1 (HCV-G1) and HCV-G3 to identify likely genotype-specific differences in lipid metabolism.
Methods

We combined several lipidomic approaches: analysis was performed between participants infected with HCV-G1 and HCV-G3, both in the fasting and non-fasting states, and after sustained virological response (SVR) to treatment. Sera were obtained from 112 fasting patients (25% with cirrhosis). Serum lipids were measured using standard enzymatic methods. Lathosterol and desmosterol were measured by gas-chromatography mass spectrometry (MS). For further metabolic insight on lipid metabolism, ultra-performance liquid chromatography MS was performed on all samples. A subgroup of 13 participants had whole body fat distribution determined using in vivo magnetic resonance imaging and spectroscopy. A second cohort of (non-fasting) sera were obtained from HCV Research UK for comparative analyses: 150 treatment naïve patients and 100 non-viraemic patients post-SVR.
Results

HCV-G3 patients had significantly decreased serum apoB, non-HDL cholesterol concentrations, and more hepatic steatosis than those with HCV-G1. HCV-G3 patients also had significantly decreased serum levels of lathosterol, without significant reductions in desmosterol. Lipidomic analysis showed lipid species associated with reverse cholesterol transport pathway in HCV-G3.
Conclusions

We demonstrated that compared to HCV-G1, HCV-G3 infection is characterised by low LDL cholesterol levels, with preferential suppression of cholesterol synthesis via lathosterol, associated with increasing hepatic steatosis. The genotype-specific lipid disturbances may shed light on genotypic variations in liver disease progression and promotion of hepatocellular cancer in HCV-G3.
Identification and Deletion of The Genes Responsible for Hydrogen Production in Thermoanaerobacter Ethanolicus JW200
Authors: Shao Xiongjun , Herring Christopher D. , Zhang Yuanxin , Zhang Gang , Tian Liang , Lynd Lee R.
Published: November 2021

Background

Thermoanaerobacter ethanolicus produces a considerable amount of ethanol from a range of carbohydrates and is an attractive candidate for applications in bioconversion processes. Due to the coupling of hydrogenase activity with fermentation product distribution, understanding hydrogen production of T. ethanolicus, particularly the genes responsible, is valuable for metabolic engineering of the species.
Results

Utilizing the hydrogenases reported in Thermoanaerobacterium saccharolyticum and Pyrococcus furiosus as templates, BLAST search identified five hydrogenase gene clusters, including two membrane-bound [NiFe] hydrogenases ech and mbh, two cytoplasmic [FeFe] hydrogenases hyd and hydII, and one cytoplasmic [NiFe] hydrogenase shi. The combined deletion of ech, mbh, shi and hydG resulted in a strain that did not produce hydrogen and showed no methyl viologen hydrogenase activity in cell extracts. Strains with deletions of all the hydrogenases except one showed normal hydrogen production. Methyl viologen hydrogenase activity was greatly reduced in all combined deletion strains except the strain with an intact hydG gene.
Conclusion

High hydrogen production and hydrogenase activities have been observed for T. ethanolicus. Five hydrogenases have been identified. Hydrogen production was eliminated by deleting genes required for all five hydrogenases. Each individual hydrogenase was verified to be capable of producing hydrogen during fermentation, indicating a high degree of redundancy and flexibility in the hydrogenase systems of T. ethanolicus. A large portion of hydrogenase activity is encoded by the [Fe-Fe] hydrogenases.
Optimum Number of Oocytes Retrieved Among Patients With Polycystic Ovary Syndrome Treated Using The Follicular Phase Long-Acting Long Protocol: A Retrospective Cohort Study
Authors: Yu Ting , Wu Di , Zhai Jun
Published: November 2021

Background: The optimum number of oocytes retrieved by the follicular phase long-acting long protocol is still unknown. This study aimed to analyze the optimum oocyte number in patients with polycystic ovary syndrome (PCOS) undergoing this protocol.Methods: A total of 1816 PCOS patients aged <35 years who were undergoing their first cycle of in vitro fertilization or intracytoplasmic sperm injection (IVF/ICSI) between January 2017 and June 2019 were identified and reviewed. All patients underwent stimulation using a follicular phase long-acting long protocol. In this retrospective cohort study, patients were categorized into seven groups according to the number of oocytes retrieved (group A, 1–5; group B, 6–10; group C, 11–15; group D, 16–20; group E, 21–25; group F, 26–30; group G, >30). The main outcome indicators were the rates of high-quality embryo, fresh cycle pregnancy, cumulative pregnancy, and “freezing all” for high ovarian response. The cumulative pregnancy and “freezing all” rates for high ovarian response were analyzed using multivariate logistic analysis.Results: The high-quality embryo rate decreased with the increase in the number of oocytes retrieved (P<0.001). In the <20 oocyte group, the clinical and cumulative pregnancy rates increased with the number of oocytes retrieved, and the “freezing all” rate for high response was within 30%. In the >20 oocyte group, with an increase in the number of oocytes retrieved, no significant change was found in the clinical and cumulative pregnancy rates (P>0.05); however, the incidence of “freezing all” rate for high response was significantly increased (P<0.001). After correcting for confounding factors, the number of oocytes retrieved was an independent predictor of the “freezing all” rate for high ovarian response (odds ratio [OR], 1.085; 95% confidence interval [CI] 1.057–1.113) and cumulative pregnancy rate (OR 1.091, 95% CI 1.057–1.126). The high-quality embryo rate was significantly affected by the cumulative pregnancy rate (OR, 59.076; 95% CI: 29.591–117.938).Conclusion: In PCOS patients aged <35 years treated using the follicular phase long-acting long protocol, considering clinical outcomes, laboratory indicators, and safety, appropriate ovarian stimulation should be used to control the number of oocytes retrieved at 11–20.
Perfil epidemiológico e distribuição demográfica do câncer de língua na região nordeste, Brasil
Authors: Ferreira Filho Mauricy Nascimento , Alves Neto Ulisses Estevam , Santos Hellen Bandeira de Pontes , Pires Andressa Cavalcanti
Published: November 2021

Introdução: A língua é uma das regiões da cavidade oral mais atingida por neoplasias malignas. Em grande parte dos casos o diagnóstico de câncer bucal é realizado tardiamente, colaborando para os altos índices de morbilidade e mortalidade dessa doença. Objetivo: Demonstrar o perfil epidemiológico e a distribuição da prevalência dos casos de câncer de língua em fumantes e ex–fumantes nos estados do nordeste brasileiro. Métodos: Trata-se de um estudo epidemiológico descritivo, transversal, realizado a partir dos dados fornecidos pelo Instituto Nacional do Câncer através dos Registros Hospitalares de Câncer, nos últimos 5 anos disponíveis, de 2013 a 2017. Todos os casos foram provenientes do Sistema Único de Saúde, separados segundo sexo e histórico de tabagismo. Os dados coletados foram analisados descritivamente. Resultados: A região do nordeste brasileiro registrou 1793 casos de câncer de língua. Os estados de Sergipe, Maranhão, Piauí apresentaram, respectivamente, os seguintes números de neoplasia na língua, 103 (5,7%), 55 (3,1%), e 2 (2,6%). Já o estado da Paraíba apresentou um total de 132 (7,4%) e Alagoas 136 (7,5%). Os estados de Pernambuco com 651(36,3%), Bahia com 489(27,3%), Rio Grande do Norte e Ceará com 173(9,6%) e 229(12,8%) respectivamente. A análise dos dados mostrou que 72,4% dos indivíduos são homens e que o hábito de parar de fumar não impediu o aparecimento da doença. Conclusão: Existe o predomínio da referida lesão no sexo masculino, cor parda e nos indivíduos tabagistas. O estado de Pernambuco apresentou o maior número de casos de câncer de língua na região nordeste e o estado do Piauí a menor prevalência da doença.
Plantas medicinais: conhecimento e uso por usuários de Unidades Básicas de Saúde em Araruna - PB, Brasil
Authors: Ferreira Sabrina Avelar Macedo , Romário-Silva Diego , Oliveira Pierre Andrade Pereira , Sette-de-Souza Pedro Henrique , Rodrigues Ana Caroline , Silva Andréa Cristina Barbosa da
Published: November 2021

Estudos etnobotânicos possibilitam o resgate e a preservação dos conhecimentos populares das comunidades, além do que essas informações podem ser utilizadas em pesquisas para produção de fitoterápicos no Brasil, que é ainda incipiente, apesar da sua enorme biodiversidade vegetal. Objetivamos realizar um estudo etnobotânico de plantas medicinais indicadas e utilizadas pela população de Araruna. Dois questionários estruturados diferenciados foram utilizados para a obtenção dos dados socioeconômicos e etnobotânicos, um para os raizeiros e outro para os usuários do Sistema Único de Saúde (SUS). A maioria dos entrevistados (66,5%) faz uso de plantas medicinais. Destes, 52,63% residem na zona rural e 47,37% na zona urbana. Além disso, 27,5% obtinham as plantas no próprio domicílio e 27% em outros locais, como na casa de parentes e amigos ou no sítio de vizinhos. Em relação a como foi adquirido o conhecimento sobre a utilização das plantas medicinais, 61% afirmaram ter obtido com a família. As plantas para fins odontológicos mais vendidas pelos raizeiros foram: boldo, camomila, canela, cajueiroroxo, alecrim, alho, babatenon, aroeira, sabugueira, cravo, romã e erva-doce. As plantas mais indicadas e utilizadas pelos usuários do Sistema Único de Saúde (SUS) foram: erva-cidreira, capim santo, boldo, sabugueira, hortelã da folha miúda, malva rosa, romã, hortelã da folha grossa, eucalipto, erva-doce, mastruz, louro, cajueiro roxo, alecrim, arruda e anador. A população de Araruna faz uso de plantas medicinais para o tratamento de diversos agravos à saúde, com conhecimento adquirido de forma popular e sem orientação profissional sobre posologia e/ou possível toxicidade dessas plantas.
Diagnóstico de queilite actínica em paciente disfuncional: relato de caso
Authors: Santos Leticia Beatriz da Cruz , Da Silva Ciro Augusto Souza Augusto Souza , Pinheiro Tiago Novaes , Vasconcelos II Antonio Jorge , Cabral Lioney Nobre
Published: November 2021

A queilite actínica (QA) é uma lesão comum do lábio inferior causada principalmente pela exposição com frequência elevada ou crônica à radiação solar. É considerada uma lesão cancerizável, onde o diagnóstico precoce pelo profissional é de extrema importância para o bom prognóstico do caso visto que a QA muitas vezes é a lesão precursora do carcinoma espinocelular de lábio inferior. Na área da saúde, é importante realizar um atendimento integral do paciente, criando uma interseção entre sua queixa principal e as necessidades que ele apresenta para que se consiga reestabelecer a saúde geral. O presente estudo visa apresentar um caso clínico de um paciente do gênero masculino, 38 anos de idade, que buscou o serviço odontológico para o tratamento de uma disfunção temporomandibular mas que, ao seu atendimento integralizado, percebeu-se a presença de uma lesão nodular em ventre lingual e de uma mácula leucoplásica em vermelhão de lábio inferior. Houve indicação para biópsia excisional da lesão nodular e de biópsia incisional para a lesão leucoplásica. Após o diagnóstico de neuroma traumático em língua e de queilite actínica em lábio inferior, com a confirmação histopatológica, foi realizada uma biópsia excisional simples. Após a remoção da lesão o paciente retornou para remoção de sutura e para proservação do caso, onde apresentou boa recuperação.
The cloverleaf plate and proximal humerus fractures
Authors: Matiotti Neto Mário , Rezende Luis Guilherme Rosifini Alves , Andrade Fernanda Ruiz de , Shimaoka Filipe Jun , Mandarano-Filho Luiz Garcia , Mazzer Nilton , Barbieri Cláudio Henrique
Published: November 2021

Background: Fractures of the proximal humerus are challenging injuries. They have a functional impairment and can be managed by non-operative or operative methods. Surgical methods can be intramedullary nails, tension-band wiring, or plates and screws. Aim of the study: This study aims to show the outcomes of managing fractures of the proximal humerus using the trefoil plate method. Methods: Eight young patients with type 2 or 3 part proximal humerus fractures were retrospectively analyzed concerning the cloverleaf plate fixation and consolidation method. Patients with 4-part fractures were excluded. Results: We obtained 100% consolidation with a mean ROM of 109 degrees for abduction, 4.4 degrees for external rotation, and good internal rotation. Conclusion: the trefoil plate osteosynthesis method can be used in young patients with 2- or 3-part fractures of the proximal humerus.
Current analysis of risk factors in maritime navigation
Authors: R. Bosneagu
Published: November 2021

any maritime voyage is exposed to sea hazards, natural and artificial dangers, as well as naval accidents and incidents. Maritime risk management issues have so far been relatively predictably connected to: natural disasters, technical faults of ships and naval equipment and human errors. Today, the particular rise in international maritime trade and the use of new technologies in the naval industry are generating new, ever-changing risks. Identifying and counteracting these risks is crucial, as the success or failure of the maritime industry can have important effects on world trade and economy. The main risk trends faced by the maritime industry at present include: natural disasters, emergence of new arctic routes, unmanned ships, autonomous transport, piracy.
Otimização do Processo de Estampagem de Peça Automotiva Utilizando Rede Neural Artificial e Algoritmo Evolutivo Multiobjetivo
Authors: Brendel Peter Laureano , Tepedino Jose Osvaldo Amaral , Miranda Fabiano , Silva Filho Jose Francisco , Parpinelli Rafael Stubs
Published: November 2021

A estampagem de peça automotiva é o processo de criar algumas partes metálicas que são utilizadas para compor a estrutura de automóveis como, por exemplo, o capô de um carro. O problema se caracteriza como problema de otimização multiobjetivo porque, para cada peça, é possível encontrar uma combinação de variáveis que impactam diferentes critérios de produção. No presente trabalho, é apresentado um modelo neuro-evolutivo para identificar valores otimizados de variáveis desse processo, de modo a atender simultaneamente três objetivos (fraturas, rugas e estiramento insuficiente) para a fabricação sem falhas. O modelo de RNA é construído utilizando dados de simulações numéricas do processo de estampagem e este modelo é utilizado pelo algoritmo NSGA-II para vasculhar o espaço de busca considerando os múltiplos objetivos. Resultados obtidos pela abordagem proposta demonstram a eficiência do modelo, contribuindo para identificação de uma solução mais eficiente para o estudo de caso experimentado.
Crear un Social Media Plan para una empresa a distancia: reto de trabajo colaborativo y de aprendizaje
Authors: Valenzuela Reyes Luz del Carmen
Published: November 2021

La educación a distancia se ha convertido en un reto para estudiantes y docentes, especialmente en el desarrollo de prácticas que permitan el contacto real del estudiante con el mundo empresarial. En este caso, a través de un proyecto integrador con el trabajo colaborativo de 3 asignaturas: Marketing Digital, Contenidos Digitales y Redes Sociales y Community Management, los estudiantes establecieron un mecanismo de asistencia a distancia, con el que trabajaron con empresas reales, quienes fueron sus clientes durante un semestre, para la planeación, implementación y evaluación y control de un Social Media Plan. Con la ayuda de las nuevas tecnologías y con el compromiso de generar una presencialidad remota efectiva, cada empresa alcanzó sus objetivos y, de esta forma, el proyecto cumplió también su objetivo de aprendizaje: Acercar a los alumnos al ambiente laboral mediante el involucramiento de los mismos en la dinámica diaria de una empresa para la elaboración de un Social Media Plan.
Effect of Carbonized Herba schizonepetae on the Bioavailability of Eight Active
Components from Pulsatillae Decoction in its Particular Adsorption and Release
Performance
Authors: Liu Cuiyan , Zhu Yun , He Yanfei , Fan Guangtai , Gu Jingang , Han Chunyang
Published: November 2021

Background:

Carbonized herbal medicine has been used clinically for centuries in China; however, its
influence on the bioavailability of compatible medicinal herbs is still unknown.

Objective:

To explore the effect of a carbonized herbal medicine on the in vivo adsorption and release and absorption
of other active pharmaceutical ingredients in a compound prescription.

Methods:

The bioavailability of carbonized Herba schizonepetae (CHS) to eight active components (epiberberine,
coptisine, palmatine, berberine, phellodendrine, aesculin, aesculetin, and anemoside B4) in the aqueous extract of
Pulsatillae Decoction (PDAE) was evaluated by the in vitro adsorption and release and in vivo pharmacokinetics
tests. Activated carbon (AC) was used as the control.

Results:

In vitro experiment showed that the cumulative adsorption rates of CHS to the eight active components were
33.17%, 54.32%, 21.48%, 42.01%, 39.1%, 25.11%, 32.11%, and 23.08% which was characterized by copsitine >
berberine > phellodendrine > epiperberine > aesculetin > anemoside B4 > palmatine., and they were significantly
lower than those of AC. The stable release concentration in sequence was 3.23, 3.04, 3.32, 7.29, 3.17, 2.80, 1.45, and
3.81 μg/mL, which was characterized by berberine > anemoside B4 > palmatine > epiberberine > phellodendrine >
coptisine > aesculin > aesculetin, and they were significantly higher than those of AC. The animal experiment indicated
that the areas under the concentration-time curve (AUC0-∞) of epiberberine, berberine, aesculetin, and anemoside
B4 in PDAE+CHS group were significantly higher than those in the PDAE and PDAE+AC groups, and the
other four components in the PDAE+CHS group were lower than those in PDAE group but higher than those in
PDAE+AC group.

Conclusion:

CHS could significantly improve the bioavailability of epiberberine, berberine, aesculetin, and anemoside
B4 in Pulsatillae Decoction and has a sustained-release effect on berberine, aesculin, aesculetin, and anemoside
B4.
A Critical Observation on the Design and Development of Reported
Peptide Inhibitors of DENV NS2B-NS3 Protease in the Last Two Decades
Authors: Murtuja Sheikh , Shilkar Deepak , Sarkar Biswatrish , Sinha Barij Nayan , Jayaprakash Venkatesan
Published: November 2021

Dengue is one of the neglected tropical diseases, which remains a reason for concern as
cases seem to rise every year. The failure of the only dengue vaccine, Dengvaxia®
, has made the problem
more severe and humanity has no immediate respite from this global burden. Dengue virus
(DENV) NS2B-NS3 protease is an attractive target partly due to its role in polyprotein processing.
Also, since it is among the most conserved domains in the viral genome, it could produce a broad
scope of opportunities toward antiviral drug discovery in general. This review has made a detailed
analysis of each case of the design and development of peptide inhibitors against DENV NS2B-NS3
protease in the last two decades. Also, we have discussed the reasons attributed to their inhibitory activity,
and wherever possible, we have highlighted the concerns raised, challenges met, and suggestions
to improve the inhibitory activity. Thus, we attempt to take the readers through the designing
and development of reported peptide inhibitors and gain insight from these developments, which
could further contribute toward strategizing the designing and development of peptide inhibitors of
DENV protease with improved properties in the coming future.
Modulation of Long Non-coding RNAs by Different Classes of Secondary
Metabolites from Plants: A Mini-review on Antitumor Effects
Authors: Almeida Tamires Cunha , Seibert Janaína Brandão , Amparo Tatiane Roquete , de Souza Gustavo Henrique Bianco , da Silva Glenda Nicioli , dos Santos Orlando David Henrique
Published: November 2021

:

The broad pharmacological spectrum of plants is related to their secondary metabolism, which is responsible for the synthesis of different compounds that have multiple effects on cellular physiology. Among the biological effects presented by phytochemicals, their use for the prevention and treatment of cancer can be highlighted. This occurs due to several mechanisms of antitumor action demonstrated by these compounds, including regulation of the cell signaling pathways and inhibition of tumor growth. In this way, long non-coding RNAs (lncRNAs) appear to be promising targets for the treatment of cancer. Their deregulation has already been related to a variety of clinical-pathological parameters. However, the effects of secondary metabolites on lncRNAs are still restricted. For this reason, the present review aimed to gather data on phytochemicals with action on lncRNAs in order to confirm their possible antitumor potential. According to the literature, terpenoid and flavonoid are the main examples of secondary metabolites involved with lncRNAs activity. In addition, the lncRNAs H19, CASC2, HOTAIR, NKILA, CCAT1, MALAT1, AFAP1-AS1, MEG3, and CDKN2B-AS1 can be highlighted as important targets in the search for new anti-tumor agents since they act as modulating pathways related to cell proliferation, cell cycle, apoptosis, cell migration and invasion. Finally, challenges for the use of natural products as a commercial drug were also discussed. The low yield, selectivity index and undesirable pharmacokinetic parameters were emphasized as a difficulty for obtaining these compounds on a large scale and for improving the potency of its biological effect. However, the synthesis and/or development of formulations were suggested as a possible approach to solve these problems. All of these data together confirm the potential of secondary metabolites as a source of new anti-tumor agents acting on lncRNAs.
Astrocyte Reactivity in Alzheimer’s Disease: Therapeutic Opportunities to Promote Repair
Authors: Mirzaei Nazanin , Davis Nicola , Chau Tsz Wing , Sastre Magdalena
Published: November 2021

:

Astrocytes are fast climbing the ladder of importance in neurodegenerative disorders, particularly in Alzheimer’s disease (AD), with the prominent presence of reactive astrocytes sur- rounding amyloid β- plaques, together with activated microglia. Reactive astrogliosis, implying morphological and molecular transformations in astrocytes, seems to precede neurodegeneration, suggesting a role in the development of the disease. Single-cell transcriptomics has recently demon- strated that astrocytes from AD brains are different from “normal” healthy astrocytes, showing dys- regulations in areas such as neurotransmitter recycling, including glutamate and GABA, and im- paired homeostatic functions. However, recent data suggest that the ablation of astrocytes in mouse models of amyloidosis results in an increase in amyloid pathology as well as in the inflammatory profile and reduced synaptic density, indicating that astrocytes mediate neuroprotective effects. The idea that interventions targeting astrocytes may have great potential for AD has therefore emerged, supported by a range of drugs and stem cell transplantation studies that have successfully shown a therapeutic effect in mouse models of AD. In this article, we review the latest reports on the role and profile of astrocytes in AD brains and how manipulation of astrocytes in animal mod- els has paved the way for the use of treatments enhancing astrocytic function as future therapeutic avenues for AD.