bluuebunny/crossref_metadata_2025 huggingface dataset:
Using LongSAGE to Detect Biomarkers of Cervical Cancer Potentially Amenable to Optical Contrast Agent Labelling
Authors: Kneller Julie M. , Ehlen Thomas , Matisic Jasenka P. , Miller Dianne , Van Niekerk Dirk , Lam Wan L. , Marra Marco , Richards-Kortum Rebecca , Follen Michelle , MacAulay Calum , Jones Steven J.M.
Published: November 2017

Sixteen longSAGE libraries from four different clinical stages of cervical intraepithelial neoplasia have enabled us to identify novel cell-surface biomarkers indicative of CIN stage. By comparing gene expression profiles of cervical tissue at early and advanced stages of CIN, several genes are identified to be novel genetic markers. We present fifty-six cell-surface gene products differentially expressed during progression of CIN. These cell surface proteins are being examined to establish their capacity for optical contrast agent binding. Contrast agent visualization will allow real-time assessment of the physiological state of the disease process bringing vast benefit to cancer care. The data discussed in this publication have been submitted to NCBIs Gene Expression Omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo/ ) and are accessible through GEO Series accession number GSE6252.
Mouse Germ Cell Developmentin-vivoandin-vitro
Authors: Saiti Deshira , Lacham-Kaplan Orly
Published: November 2017

In mammalian development, primordial germ cells (PGCs) represent the initial population of cells that are committed to the germ cell lineage. PGCs segregate early in development, triggered by signals from the extra-embryonic ectoderm. They are distinguished from surrounding cells by their unique gene expression patterns. Some of the more common genes used to identify them are Blimp1, Oct3/4, Fragilis, Stella, c-Kit, Mvh, Dazl and Gcna1. These genes are involved in regulating their migration and differentiation, and in maintaining the pluripotency of these cells.

Recent research has demonstrated the possibility of obtaining PGCs, and subsequently, mature germ cells from a starting population of embryonic stem cells (ESCs) in culture. This phenomenon has been investigated using a variety of methods, and ESC lines of both mouse and human origin. Embryonic stem cells can differentiate into germ cells of both the male and female phenotype and in one case has resulted in the birth of live pups from the fertilization of oocytes with ESC derived sperm. This finding leads to the prospect of using ESC derived germ cells as a treatment for sterility. This review outlines the evolvement of germ cells from ESCs in vitro in relation to in vivo events.
Electron Pathways through Erythrocyte Plasma Membrane in Human Physiology and Pathology: Potential Redox Biomarker?
Authors: Matteucci Elena , Giampietro Ottavio
Published: November 2017

Erythrocytes are involved in the transport of oxygen and carbon dioxide in the body. Since pH is the influential factor in the Bohr-Haldane effect, pHi is actively maintained via secondary active transports Na+/H+exchange and HC3–/Cl–anion exchanger. Because of the redox properties of the iron, hemoglobin generates reactive oxygen species and thus, the human erythrocyte is constantly exposed to oxidative damage. Although the adult erythrocyte lacks protein synthesis and cannot restore damaged proteins, it is equipped with high activity of protective enzymes. Redox changes in the cell initiate various signalling pathways. Plasma membrane oxido-reductases (PMORs) are trans-membrane electron transport systems that have been found in the membranes of all cells and have been extensively characterized in the human erythrocyte. Erythrocyte PMORs transfer reducing equivalents from intracellular reductants to extracellular oxidants, thus their most important role seems to be to enable the cell respond to changes in intra- and extra-cellular redox environments.

So far the activity of erythrocyte PMORs in disease states has not been systematically investigated. This review summarizes present knowledge on erythrocyte electron transfer activity in humans (health, type 1 diabetes, diabetic nephropathy, and chronic uremia) and hypothesizes an integrated model of the functional organization of erythrocyte plasma membrane where electron pathways work in parallel with transport metabolons to maintain redox homeostasis.
Gene Expression Profiles from Needle Biopsies Provide Useful Signatures of Non-Small Cell Lung Carcinomas
Authors: Paull Douglas E. , Kelley Kevin , Moezzi Jazbieh , Kadakia Madhavi , Berberich Steven J.
Published: November 2017

Gene expression profiles from DNA microarrays can provide molecular signatures that improve tumor classification, prognosis, and treatment options. While much of this work has focused on isolation of RNA from the resected tumor, fewer studies have utilized RNA from fine needle aspirates (FNA). In this pilot study we examined whether the gene signatures obtained from FNA samples would correlate with signatures taken from the resected tumor. Based on NSCLC gene expression profiles obtained from eleven sets of FNA and tumor samples we obtained a high concordance of FNA profiles matching their matched tumor sample. These results suggest that FNA samples may provide informative gene expression signatures regarding the potential aggressiveness of non-small-cell lung carcinomas.
Methodology and Applications of Disease Biomarker Identification in Human Serum
Authors: Sahab Ziad J. , Semaan Suzan M. , Sang Qing-Xiang Amy
Published: November 2017

Biomarkers are biomolecules that serve as indicators of biological and pathological processes, or physiological and pharmacological responses to a drug treatment. Because of the high abundance of albumin and heterogeneity of plasma lipoproteins and glycoproteins, biomarkers are difficult to identify in human serum. Due to the clinical significance the identification of disease biomarkers in serum holds great promise for personalized medicine, especially for disease diagnosis and prognosis. This review summarizes some common and emerging proteomics techniques utilized in the separation of serum samples and identification of disease signatures. The practical application of each protein separation or identification technique is analyzed using specific examples. Biomarkers of cancers of prostate, breast, ovary, and lung in human serum have been reviewed, as well as those of heart disease, arthritis, asthma, and cystic fibrosis. Despite the advancement of technology few biomarkers have been approved by the Food and Drug Administration for disease diagnosis and prognosis due to the complexity of structure and function of protein biomarkers and lack of high sensitivity, specificity, and reproducibility for those putative biomarkers. The combination of different types of technologies and statistical analysis may provide more effective methods to identify and validate new disease biomarkers in blood.
Multianalyte Tests for the Early Detection of Cancer: Speedbumps and Barriers
Authors: Tainsky Michael A. , Chatterjee Madhumita , Levin Nancy K. , Draghici Sorin , Abrams Judith
Published: November 2017

It has become very clear that a single molecular event is inadequate to accurately predict the biology (or pathophysiology) of cancer. Furthermore, using any single molecular event as a biomarker for the early detection of malignancy may not comprehensively identify the majority of individuals with that disease. Therefore, the fact that technologies have arisen that can simultaneously detect several, possibly hundreds, of biomarkers has propelled the field towards the development of multianalyte-based in vitro diagnostic early detection tests for cancer using body fluids such as serum, plasma, sputum, saliva, or urine. These multianalyte tests may be based on the detection of serum autoantibodies to tumor antigens, the presence of cancer-related proteins in serum, or the presence of tumor-specific genomic changes that appear in plasma as free DNA. The implementation of non-invasive diagnostic approaches to detect early stage cancer may provide the physician with evidence of cancer, but the question arises as to how the information will affect the pathway of clinical intervention. The confirmation of a positive result from an in vitro diagnostic cancer test may involve relatively invasive procedures to establish a true cancer diagnosis. If in vitro diagnostic tests are proven to be both specific, i.e. rarely produce false positive results due to unrelated conditions, and sufficiently sensitive, i.e. rarely produce false negative results, then such screening tests offer the potential for early detection and personalized therapeutics using multiple disease-related targets with convenient and non-invasive means. Here we discuss the technical and regulatory barriers inherent in development of clinical multianalyte biomarker assays.
Cellular Adhesion Molecules in Healthy Subjects: Short Term Variations and Relations to Flow Mediated Dilation
Authors: Eschen Ole , Christensen Jeppe Hagstrup , Dethlefsen Claus , Schmidt Erik Berg
Published: November 2017

The objective was primarily to describe short term intra-individual variation in serum levels of soluble adhesion molecules (sCAMs: E-selectin, P-selectin, intercellular adhesion molecule-1(sICAM-1) and vascular cellular adhesion molecule-1(sVCAM-1)) in healthy subjects. Secondly, sCAMs were correlated to brachial artery flow mediated vasodilation (FMD).

Forty healthy subjects aged 24–66 years had sCAMs measured twice with 4 week intervals and short-term intra-individual variation was estimated as variation in the paired measurements after correcting for the analytical precision of the used method. At baseline, brachial FMD was measured.

No difference was observed in mean sCAMs in the whole study group. Estimated intra-subject variations in sCAMs were 7.6–11.3%. In a regression analysis, significant negative association was found between sE-selectin and FMD after controlling for possible confounders (p < 0.04) while no significant correlation could be demonstrated between the other sCAMs and FMD.

In conclusion, short term intra-individual variations in sCAMs were 7.6–11.3% in healthy subjects. We also found a significant negative association between sE-selectin and FMD, indicating an possible association between inflammation and dysfunction of the vascular endothelium; however further studies are required to confirm this preliminary finding.
Novel Biomarkers Associated with Deep Venous Thrombosis: A Comprehensive Review
Authors: Barnes Dawn M , Wakefield Thomas W , Rectenwald John E
Published: November 2017

Primary and recurrent venous thromboembolic disease (VTE, deep venous thrombosis and pulmonary embolism) remain a significant source of morbidity and mortality in the hospitalized patient. Non-specific subjective complaints and lack of specific objective findings related to acute deep venous thrombosis (DVT) and pulmonary embolism (PE) complicate the diagnosis. There remains no single serum marker available to exclusively confirm the diagnosis of VTE. While D-dimer is highly sensitive and useful for diagnostic exclusion, it lacks the specificity necessary for diagnostic confirmation resulting in the need for a variety of additional studies (i.e.: duplex ultrasound, venography, V/Q scanning, helical thoracic and pelvic CT scans and pulmoary angiography). There is evolving research supporting the utility of various plasma markers as novel “biomarkers” for VTE including selectins, microparticles, interleukin-10 and other cytokines. This review attempts to examine recent literature assessing the utility of P-selectin, microparticles, D-dimer, E-selectin, thrombin, interleukins and fibrin monomers in the diagnosis and guidance of therapy for VTE.
A game of thrones: Organising and legitimising knowledge through PISA research
Authors: Mølstad Christina E , Pettersson Daniel , Forsberg Eva
Published: November 2017

This study investigates knowledge structures and scientific communication using bibliometric methods to explore scientific knowledge production and dissemination. The aim is to develop knowledge about this growing field by investigating studies using international large-scale assessment (ILSA) data, with a specific focus on those using Programme for International Student Assessment (PISA) data. As international organisations use ILSA to measure, assess and compare the success of national education systems, it is important to study this specific knowledge to understand how it is organised and legitimised within research. The findings show an interchange of legitimisation, where major actors from the USA and other English-speaking and westernised countries determine the academic discourse. Important epistemic cultures for PISA research are identified: the most important of which are situated within psychology and education. These two research environments are epicentres created by patterns of the referrals to and referencing of articles framing the formulation of PISA knowledge. Finally, it is argued that this particular PISA research is self-referential and self-authorising, which raises questions about whether research accountability leads to ‘a game of thrones’, where rivalry going on within the scientific field concerning how and on what grounds ‘facts’ and ‘truths’ are constructed, as a continuing process with no obvious winner.
Interrelationship of canonical and non-canonical Wnt signalling pathways in chronic metabolic diseases
Authors: Ackers Ian , Malgor Ramiro
Published: November 2017

Chronic diseases account for approximately 45% of all deaths in developed countries and are particularly prevalent in countries with the most sophisticated and robust public health systems. Chronic metabolic diseases, specifically lifestyle-related diseases pertaining to diet and exercise, continue to be difficult to treat clinically. The most prevalent of these chronic metabolic diseases include obesity, diabetes, non-alcoholic fatty liver disease, chronic kidney disease and cardiovascular disease and will be the focus of this review. Wnt proteins are highly conserved glycoproteins best known for their role in development and homeostasis of tissues. Given the importance of Wnt signalling in homeostasis, aberrant Wnt signalling likely regulates metabolic processes and may contribute to the development of chronic metabolic diseases. Expression of Wnt proteins and dysfunctional Wnt signalling has been reported in multiple chronic diseases. It is interesting to speculate about an interrelationship between the Wnt signalling pathways as a potential pathological mechanism in chronic metabolic diseases. The aim of this review is to summarize reported findings on the contrasting roles of Wnt signalling in lifestyle-related chronic metabolic diseases; specifically, the contribution of Wnt signalling to lipid accumulation, fibrosis and chronic low-grade inflammation.
Legislative Term Limits and Voter Turnout
Authors: Kuhlmann Robynn , Lewis Daniel C.
Published: November 2017

According to reformers, legislative term limits should increase voter turnout by enhancing electoral competitiveness for legislative seats. However, this claim has been largely untested. The only existing study of the effect of legislative term limits on voter turnout, to date, finds that turnout in California did not increase after the imposition of term limits and may have decreased turnout. Yet, it is unclear whether this result generalizes to other states. This study employs a comparative state analysis of both aggregate turnout and district-level turnout rates in state legislative elections. We find that term limits significantly increase voting rates in state legislative elections.
Evaluating Policy Representation with Dynamic MRP Estimates: Direct Democracy and Same-Sex Relationship Policies in the United States
Authors: Lewis Daniel C. , Jacobsmeier Matthew L.
Published: November 2017

Does direct democracy strengthen popular control of public policy in the United States? A major challenge in evaluating policy representation is the measurement of state-level public opinion and public policy. Although recent studies of policy responsiveness and congruence have provided improved measures of public opinion using multilevel regression and poststratification (MRP) techniques, these analyses are limited by their static nature and cross-sectional design. Issue attitudes, unlike more general political orientations, often vary considerably over time. Unless the dynamics of issue-specific public opinion are appropriately incorporated into the analyses, tests of policy responsiveness and congruence may be misleading. Thus, we assess the degree of policy representation in direct democracy states regarding same-sex relationship recognition policies using dynamic models of policy adoption and congruence that employ dynamic MRP estimates of attitudes toward same-sex marriage. We find that direct democracy institutions increase both policy responsiveness and congruence with issue-specific public opinion.
Silica nanoparticles induce NLRP3 inflammasome activation in human primary immune cells
Authors: Gómez Diana M , Urcuqui-Inchima Silvio , Hernandez Juan C
Published: November 2017

In recent years, the potential use of silica nanoparticles (SiNPs) among different biomedical fields has grown. A deep understanding of the physicochemical properties of nanoparticles (NPs) and their regulation of specific biological responses is crucial for the successful application of NPs. Exposure to NP physicochemical properties (size, shape, porosity, etc.) could result in deleterious effects on cellular functions, including a pro-inflammatory response mediated via activation of the NLRP3 inflammasome. The aim of this study was to evaluate the potential in vitro immunomodulatory effect of 12-nm and 200-nm SiNPs on the expression of pro-inflammatory cytokines and NLRP3 inflammasome components in human primary neutrophils and PBMCs. This study demonstrates that regardless of the size of the nanoparticles, SiNPs induce the production of pro-inflammatory cytokines in a dose-dependent manner. Induced IL-1β production after exposure to SiNPs suggests the involvement of NLRP3 inflammasome components participation in this process. In conclusion, SiNPs induce the production of pro-inflammatory cytokines in a dose-dependent manner. Furthermore, our data suggest that the production and release of IL-1β possibly occurs through the formation of the NLRP3 inflammasome.
Human health and social-ecological systems change: Rethinking health in the Anthropocene
Authors: Zywert Katharine
Published: November 2017

This review article considers how social-ecological systems change is transforming human health in the Anthropocene. From hunting and gathering bands through modern globalized societies, human health has been shaped by circular feedbacks between ecological processes, available energy sources, levels of social complexity, and cultural ontologies. As the environmental crises of the early Anthropocene (biodiversity loss, climate change, land use changes) push ecosystems across thresholds into new configurations, we are experiencing an equally profound transition for human health. Drawing on literatures from medical anthropology, sociology, complexity science, and ecological economics, this article argues that promising alternatives for health systems in the Anthropocene are emerging beyond the boundaries of the formal healthcare sector in community-based practices that can take root in a context of ecological limits, economic contraction, and growing networks of reciprocal care.
X-Linked Lissencephaly With Absent Corpus Callosum and Abnormal Genitalia
Authors: Coman David , Fullston Tom , Shoubridge Cheryl , Leventer Richard , Wong Flora , Nazaretian Simon , Simpson Ian , Gecz Josef , McGillivray George
Published: November 2017

X-linked lissencephaly with abnormal genitalia is a rare and devastating syndrome. The authors present an infant with a multisystem phenotype where the intestinal manifestations were as life limiting as the central nervous system features. Severe chronic diarrhea resulted in failure to thrive, dehydration, electrolyte derangements, long-term hospitalization, and prompted transition to palliative care. Other multisystem manifestations included megacolon, colitis, pancreatic insufficiency hypothalamic dysfunction, hypothyroidism, and hypophosphatasia. A novel aristaless-related homeobox gene mutation, c.1136G>T/p.R379L, was identified. This case contributes to the clinical, histological, and molecular understanding of the multisystem nature of this disorder, especially the role of ARX in the development of the enteroendocrine system.
First record of Stenodiplosis sorghicola on sorghum in Greece
Authors: Tsagkarakis A. E. , Emmanouel N. G. , Scarakis G. N.
Published: November 2017

In October 2007, the sorghum midge Stenodiplosis sorghicola (Coquillett) (Diptera: Cecidomyiidae) was recorded for the first time in Greece. Its occurrence was noticed in an experimental sorghum cultivar at Aliartos, near Athens, in Viotia region. Information on its morphology, biology and distribution is given.
Bio-Ecological Research of Lily Leaf Beetle Lilioceris lilii Scopoli (Coleoptera: Chrysomelidae) in Bosnia and Herzegovina
Authors: Brzica Mirjana
Published: November 2017

In the present study some bio-ecological characteristics of Lilioceris lilii in Bosnia and Herzegovina are presented. The insect’s presence was confirmed in the majority of the researched locations in Bosnia and Herzegovina. The average body length of L. lilii was 7.64 mm (7.025 mm for the males while 8.106 mm for the females) and the average body width 3.75 mm. The average length of a fully developed larva was 6.36 mm, and the average width was 2.69 mm. In terms of the cocoon, the average length was 8.83 mm and its width was 5.82 mm. Duration of embryonic development under field conditions was 8.5 days in average. The average duration of larval development (4 instars) was 16 days and the pupal stage lasted approximately 18 days. Lilioceris lilii retreated into winter quiescence at the beginning of autumn, thereof completes one generation per year. In the area of Sarajevo in field conditions the number of the laid eggs per female was 130 in average. The insect undergoes its entire development at the Asian and Oriental species of lily, on all the above-ground parts of the plants. Lilium candidum lily species that was present in all home gardens in the observed areas was the major host of the L. lilii. Experimentation with Aloae vera proved that this species was not a host of L. lilii.
Temperature dependent development of Phenacoccus solenopsis under laboratory conditions
Authors: Kumar Sanjeev , Kontodimas Ch.
Published: November 2017

Cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) is an important cotton pest in Punjab, India. Development of the immature stages (four instars in female and five in male) of P. solenopsis was observed at nine constant temperatures (15, 18, 20, 25, 30, 34, 35, 38, 40°C). Using the linear model, the lower temperature threshold (tmin) for development was estimated to be 4.93 and 2.95°C and the thermal constant (K) was 333.33 and 454.54 degree days for female and male, respectively. In addition, three nonlinear models (Lactin, Bieri-1 and 3rd order polynomial) were tested to describe the relationship between temperature and development rate and to estimate the upper developmental threshold (tmax) and the optimum temperature for development (topt). Our results proved that the Bieri-1 and Lactin models and provided the best fit (r2 = 93,4 – 99,9%) and estimated accurately all the three critical temperatures, ranged tmin = 5.06 – 5.25°C, topt = 33.55 – 33.60°C, tmax = 39.99 – 40.00°C, for the total development of females and tmin = 2.82 – 3.16°C, topt = 34.01 – 34.04°C, tmax =40.00 – 40.10°C, for the total development of males.
New records of aphid species (Hemiptera: Aphididae) in Greece
Authors: Papapanagiotou A. P. , Nathanailidou M. , Taylor M. , Zarpas K. D. , Voudouris K. , Tsitsipis J. A. , Margaritopoulos J. T.
Published: November 2017

Several papers have been published on aphid fauna in Greece during the last two decades, but the number of recorded species is still low compared to other European countries, including some from the Mediterranean basin. In this context, we collected aphids from various host-plants and regions in southern, central and northern Greece characterized by diverse flora, climatic conditions and ecological habitats. In total, 128 aphid species belonging to 55 genera and six subfamilies were collected on 200 host-species. Most of the species dominated the subfamily Aphidinae (especially tribes Macrosiphini and Aphidini). Among the species collected, 18 were new records in Greece. The present work improves our knowledge regarding the aphid fauna of Greece and suggests that the number of recorded species could increase further if additional studies were undertaken.
First record of Lilioceris lilii in Greece
Authors: Papadoulis Georgios Th. , Tsagkarakis Antonios E.
Published: November 2017

On May 2007, the lily beetle Lilioceris lilii (Scopoli) (Coleoptera: Chrysomelidae) was recorded for the first time in Greece. Its occurrence was observed on scarlet martagon lily and martagon lily plants at Fylaktí, Karditsa. Information on its morphology, biology and distribution is presented.
Review of Aulacidae from Greece and Cyprus with new records
Authors: Turrisi Giuseppe Fabrizio
Published: November 2017

The Aulacidae (Hymenoptera: Evanioidea) from Greece and Cyprus are reviewed. Seven species are recorded, all comprised within the genus Pristaulacus Kieffer, 1900. Two of them, P. chlapowskii Kieffer, 1900 and P. compressus (Spinola, 1808) are reported for the first time from Greece; P. mourguesi Maneval, 1935, previously known from only one locality of northern Greece, is recorded for the first time from the Eastern Aegean islands (Ikaria) and other localities from the Greek mainland are reported; P. galitae (Gribodo, 1879) is recorded for the first time from Lesvos island (Eastern Aegean islands) and Cyprus. Brief references for identification and essential data on the treated species are provided.
Myrmecophilus balcanicus, a new species of ant-loving cricket from the Former Yugoslav Republic of Macedonia, with notes on the synonymy of Myrmecophilus zorae
Authors: Stalling Thomas
Published: November 2017

A new species of ant-loving cricket, Myrmecophilus balcanicus sp. n., is described and illustrated based on individuals collected in the Former Yugoslav Republic of Macedonia (FYROM). It’s habitat is described. The species belongs to the subgenus Myrmecophilus Berthold, 1827. Myrmecophilus zorae Karaman, 1963 is recognized as a junior synonym of Myrmecophilus hirticaudus Fischer von Waldheim, 1846.
Structure of soil biocoenosis and biodiversity in olive orchards of three eastern Mediterranean islands
Authors: Liantraki Z. , Tzokas I. , Kollaros D.
Published: November 2017

In this work the structure of soil arthropods’ biocoenosis was compared among 11 olive orchards from six localities of Crete (three of the localities from Messara’s valley, including two olive orchards each one, plus three localities near to Herakleion city) and other two ones each from the island of Kos and Cyprus. Pitfall traps were used and totally 35 taxa were captured. The order Coleoptera and the family Formicidae were the two main taxa at the 8 of the 11 olive orchards. Statistically significant differences in biodiversity were recorded among the orchards separating them in three groups, the first group included Messara’s olive orchards, the second the olive orchards of the rest of the Crete and the third group the olive groves of the other two islands. Messara’s group had higher biodiversity than the third group. By studying the similarities of biological communities, some pairs of Messara’s olive groves, have the highest ones. Among the 15 highest values, the 14 belonged to pairs between Messara’s olive orchards. Generally, biocoenosis in all 11 olive orchards of the three different islands follows a similar pattern, despite of differences in cultural practices and changes of climatic factors from a year to another.
Comparison of coleopteran fauna in olive orchards under different production systems in the Messara’s valley, on Crete island, Greece
Authors: Tzokas I. , Liantraki Z. , Kollaros D.
Published: November 2017

In this study the results of the captured Coleoptera in pitfall traps in nine olive orchards in the valley of Messara, on Crete Island, were compared. The liquid used for killing and preservation in the pitfall traps, was propylene glycol. The nine olive orchards were distributed in four areas in the valley. In each area there were at least two olive orchards under different agricultural  management system, organic and conventional. The experiments were conducted from October 2004 to April 2005. The number of captured Coleoptera specimens was 4,937. The specimens were identified to the family level. Coleoptera from 25 families were found, but the analysis was focused at eleven of them, the richest in specimens. These were: Anthicidae, Carabidae, Chrysomelidae, Curculionidae, Histeridae, Leiodidae, Scarabaeidae, Silphidae, Silvanidae, Staphylinidae and Tenebrionidae. Staphylinidae were the most abundant in all olive orchards independently of the agricultural management (production system). In the other families instead of differences amongst the different systems of agricultural management, more prominent were the differences amongst areas.
Sakis Drosopoulos: Obituary
Authors: Emmanouel N. G. , Tsagkarakis A. E.
Published: November 2017

No abstract (available).
Study on the combined action of the entomopathogenic bacterium Bacillus thuringiensis subsp. kurstaki and the entomopathogenic nematode Heterorhabditis bacteriophora
Authors: Zampara I. , Zamparas C. , Mantzoukas S. , Karanastasi E.
Published: November 2017

The interaction between the entomopathogenic bacterium Bacillus thuringiensis subsp. Kurstaki and the entomopathogenic nematode Heterorhabditis bacteriophora (Heterorhabditidae) was examined against larvae of Ephestia kuehniella (Lepidoptera: Pyralidae) at 7, 14, 21 and 28 days post treatment, in laboratory conditions. Three different combinations of the aforementioned pathogens were tested on 4th instar larvae, namely 500ppm B. thuringiensis subsp. kurstaki (B.t.k.) and H. bacteriophora infective Juveniles (1000IJs/ml), 1500ppm B.t.k. and H. bacteriophora (1000IJs/ml) and 3000ppm B.t.k. and H. bacteriophora (1000IJs/ml). At 7, 14 and 21 days, the interaction between the pathogens was additive in two of the treatments and synergistic in one, whereas at 28 days, it was negative in two of the treatments and synergistic in one. Overall, the application of the lowest dose of B.t.k. (500ppm) in combination with H. bacteriophora (1000IJs/ml), turned out to be highly effective. The interaction between B. thuringiensis and H. bacteriophora is to be further examined.
Varietal performance of cabbage on the incidence of flea beetles (Phyllotreta spp.)
Authors: Islam M. R. , Ali M. R. , Ahmed W. R. , Rahman M. M. , Hira H.
Published: November 2017

A study was conducted at the Sher-e-Bangla Agricultural University, Bangladesh, during September 2007 to February 2008 to evaluate the varietal performance of cabbage on the incidence of flea beetles [Phyllotreta spp., (Coleoptera: Chrysomelidae)]. The experiment was laid out in a Randomized Complete Block Design comprising seven varieties viz. V1: BARI badhakopi-1/Provati, V2: BARI badhakopi-2/Agrodot, V3: Atlas-70, V4: Autumn queen, V5: Tropical queen, V6: T-776 and V7: Seisho YR. Among the different varieties of cabbage V5 (Tropical queen) performed as the best variety in terms of the lowest incidence of flea beetles (0.00 larvae/plant), lowest percent of leaves infestation (0.87 %) as well as plant infestation (2 %) caused by flea beetles. These findings will be valuable for the most appropriate management of flea beetles.
First record of the sisal weevil, Scyphophorus acupunctatus, in Cyprus
Authors: Vassiliou Vassilis , Kitsis Pavlos
Published: November 2017

In May 2013, numerous adults of a coleopteran insect species that later was identified as the sisal weevil, Scyphophorus acupunctatus (Coleoptera: Curculionidae), were accidentally captured in both pitfall and funnel traps placed across Cyprus for the monitoring of red palm weevil, Rhynchophorus ferrugineus (Coleoptera: Curculionidae). S. acupunctatus was found for the first time in the Germasogeia area of the Limassol district (34°71′81″N, 33°08′56″E) and in the Kissonerga area of the Paphos district (34°81′67″N, 32°40′00″E). During 2013 and early 2014, numerous adults of this species were also collected from red palm weevil traps from all over Cyprus
First report of the bordered straw, Heliothis peltigera, οn sunflower in Greece
Authors: Simoglou K. B. , Anastasiades A. I. , Baixeras J. , Roditakis E.
Published: November 2017

In June 2015, infestations of sunflower crops by the bordered straw Heliothis peltigera (Lepidoptera: Noctuidae) were recorded in rural areas of the Regional Unit of Drama, N. Greece. Larval feeding-damage symptoms were observed on leaves and buds of sunflower plants, mostly at pre-flowering growth stages. Infestations were recorded in approximately 80% of the inspected sunflower fields, the infestation levels, however, were low (<5%) apparently resulting in no economic damage. The presence of H. peltigera has been reported in Southern and Central Europe, Canada, the Middle East and Africa, infesting several hosts. Infestations of sunflower crops have been reported in India, Pakistan and Sudan. To our knowledge, this is the first report of H. peltigera infestations on sunflower crops in Greece and possibly in Europe.
Fauna of Encyrtidae, parasitoids of coccids in Golcuk Natural Park
Authors: Japoshvili G. , Celik H.
Published: November 2017

Species of Encyrtidae (Hymenoptera: Chalcidoidea), which are parasitoids of Coccoidea, were collected by malaise trap in Golcuk Natural Park during March-October, 2009. Forty-four species of encyrtids, belonging to 24 genera, were recorded during our survey. Two genera and nine species were found to represent new records for the Turkish fauna. Twenty-eight encyrtid species were identified to the species level and the remaining specimens to genus and morphospecies level. This was the first attempt at estimating the scale insect fauna using their parasitoids collected by malaise traps.
Obituary: Helen May Brookes (3.11.1917-1.1.2008)
Authors: Gullan P.J. , Williams D.J.
Published: November 2017

[...]
Obituary: Dr. A. Samanidou-Voyadjoglou (20.5.1946-21.8.2009)
Authors: Vakalis N.
Published: November 2017

[...]
Wolbachia-induced reproductive parasitism and applications
Authors: Saridaki A. , Bourtzis K.
Published: November 2017

Insects have been reported to be associated with a broad variety of microorganisms, affecting the host biology in many different ways. Among them, Wolbachia, an obligatory intracellular and maternally-inherited symbiont, has recently attracted a lot of attention. Beside insects, Wolbachia are found in association with a wide variety of other invertebrate species, including mites, scorpions, spiders, crustaceans, filarial nematodes. Several surveys have indicated that Wolbachia may be symbiont of up to 70% of all insect species, rendering Wolbachia the most ubiquitous intracellular symbiotic organism on Earth. Wolbachia-host interactions range from many forms of reproductive parasitism to mutualistic symbioses. Different Wolbachia strains have been found to induce a number of reproductive alterations such as feminization, parthenogenesis, male-killing or cytoplasmic incompatibility. Despite their common occurrence and major effects on host biology, speciation and ecological diversity, little is known on the molecular mechanisms that mediate Wolbachia-host interactions. Recent studies focus on the potential of Wolbachia-based methods for the biological control of insect pests and disease vectors of agricultural, environmental and medical importance.
The status of Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) in Greece: A case of an exotic predator that failed to establish?
Authors: Kontodimas D. C. , Stathas G. J. , Martinou A. F.
Published: November 2017

During 1994 -1999 several hundred thousands of Harmonia axyridis adults were released at various cultivations infested by aphids (citrus, vegetable and bean crops, maize, etc.) or in urban places on ornamental plants in central and southern Greece (mainly Attica and Peloponnessos region) as well as on several islands. During 1995-2007, samplings were conducted in some areas, in spring just before any new releases, in order to determine if H. axyridis overwintered in the field. In spring 1995 (the year that followed the first releases) as well in spring 1996-97 and 2000-07, no presence of H. axyridis was recorded in any of the orchards where the predator had been released. Only in spring 1998 and 1999 small colonies (<50 individuals) of overwintered H. axyridis adults were observed in Attica region. The above results indicate an inability of the H. axyridis released populations to establish in Greece, although in some areas this species became an important biocontrol agent during the season. In addition, some data collected regarding the composition of native coccinelid species pre- and post- release of H. axyridis could be an indication of no adverse effects imposed by H. axyridis on the native coccinellid fauna in Greece. Despite that, any re-initiation of releases of H. axyridis would not be wise because of its multiple negative effects observed in other countries of Europe and the USA. Contrary to this, the application of a survey and monitoring programme that will allow us to be prepared in case of a possible invasion of H. axyridis from northern Europe is suggested.
First record of Monarthropalpus buxi in Greece and some data of its phenology
Authors: Vamvakas M. , Kontodimas D.C. , Milonas P.G.
Published: November 2017

The gall inducing insect Monarthropalpus buxi (Laboulbene) (Diptera: Cecidomyiidae) has been recorded in Greece on 20/4/2004 in Athens (Ellinikon, GPS point: N 370 54’ 22”, E 23° 44’ 34”) on Buxus roduntifolia. Two-20 larvae per infested leaf were noticed but only 2-3 adults per leaf emerged. The majority (>92%) of the population of M. buxi completed one generation per year and adults emerged from late April to early May. A small proportion (<8%) of the population completed two generations per year and I generation adults emerged from late January to late March. Second generation adults emerged from late June to late July. In addition, during 2006 parasitism by Torymus sp. (Hymenoptera: Torymidae) on larvae of the fly has been noticed.
Changes in the total protein, carbohydtate and lipid contents in selected tissues of silkworm, Bombyx mori L. under the influence of a juvenoid R394
Authors: Nair K. Sashindran , Nair Jula S. , Vijayan V.A.
Published: November 2017

A juvenoid. R394 (Ethyl 9-cyclohexyl-3,7-dimethyl-2,4-nonadienoate) was applied topically to 5th instar silkworm, Bombyx mori L. larvae (Hybrid: KA x NB4D2) at a dose of 0.039 nl/larva at 24, 48, 72 and 96 h, for silk yield improvement. Three major selected tissues viz., posterior silk gland (PSG), haemolymph and fat body were collected from fully-grown larvae and the total protein, total carbohydrate and total lipid contents were estimated following standard procedures. The result indicated that the content of these primary metabolites varied significantly in the selected tissues depending on the time of juvenoid application. The highest protein content was observed in the haemolymph and silk gland in the larvae treated at 72 h whereas the fat body protein content was lowest for the same treatment. The total carbohydrate was recorded lowest in the 72 h treated larvae as against the highest in the control both in haemolymph and fat body with no significant change in PSG. The total lipid content did not show any notable variation in the concentration on juvenoid administration except in the silk gland treated up to 72 h which showed a decline. The results indicate that the juvenoid induces tissue-specific responses in terms of turnover in primary metabolites which commensurates with the corresponding changes observed in the cocoon weight and cocoon shell weight.
Definition of timing in the control of the 1st generation Lasioderma serricorne adults, inside tobacco stores, based on the development of the oocytes
Authors: Papadopoulou Smaragdi C.
Published: November 2017

The development of the oocytes of pupae, females inside the pupal cell and after emergence of Lasioderma serricorne (F.) (Coleoptera: Anobiidae) was studied by dissecting their abdomen. The time of oocytes maturation as well as that of the starting of their oviposition, in combination with the insect’s developmental threshold was determined, aiming to time the 1st effective control against the emerging adults of the 1st generation.
Establishment of economic injury levels for olive infestation by Dαcus oleαe, in Corfu
Authors: Kapatos Ε.Τ. , Fletcher B.S.
Published: November 2017

Economic injury levels for cover sprays and air bait sprays were established for the infestation by Dacus oleae (Gmelin) (Diptera: Tephritidae). For cover sprays, the economic injury levels (expressed as percent infestation by taking 30,000 fruits to represent the average number of fruits per tree) were calculated as 7.59% for infestation laid in late July-August, 6.16% for infestation in September and 10.31% for infestation in October. For air bait sprays, economic injury levels were calculated for various fruiting conditions (expressed as proportion of trees bearing olive fruits) and they were much lower than for cover sprays due to the lower cost of air treatments. For July-August, they ranged from 5.07% infestation for 25% trees bearing olives to 1 .27% for 100% trees with olive fruits. For September they ranged from 4.11% to 1.08% infestation, for October they ranged from 6.88% to 1 .72% infestation and for infestation laid in November they ranged from 61.28% to 15.32% infestation, respectively. The economic injury levels for air bait sprays were also expressed as mean weekly number of females per McPhail trap by taking into account the potential fecundity of D. oleae and the efficiency of the McPhail trap (baited with protein hydrolysate 2% and borax 1 .5%) at different times of the year. For September, they ranged from 16 females per trap for groves with 25% of trees bearing olive fruits, to 4 females per trap for 100% trees with fruits. For October, they ranged from 6 females per trap to I female per trap, respectively.
Nathrius brevipennis (Mu1sant 1839) (Col.: Cerambycidae) in Greece
Authors: Santas L.A.
Published: November 2017

In the spring of 1975, the trees of an almond orchard [Prunus dulcis (Miller) D.A. Webb] in Avliotes Corfu, were found to be severely infested by a wood borer insect in the larval stage. Samples of infested with larvae twigs, preserved in the laboratory, and in the same year in September a number of adults emerged. After identification this insect was found to be Nathrius (Leptidea) brevipennis (Mulsant 1839). This species caused important damage in the almond trees of the Avliotes area of Corfu during the years 1975-1976. In 1977, N. brevipennis was found in Ioannina county on walnut trees (Juglans regia L.) and in 1980, it was found in Metsovo area on almond trees.
Anapulvinaria pistaciae (Bod.), a pistachio tree scale pest producing honeydew foraged by bees in Greece
Authors: Santas L.A.
Published: November 2017

The soft scale Anapulvinaria pistaciae (Bod.), a pest of pistachio, excretes honeydew which is exploited by honeybees. The scale has one generation per year. Adults appear early in spring and crawlers in May. From mid-May to mid-June the population of A. pistaciae consists mainly of crawlers and/or first stage nymphs. The second stage nymphs appear early in fall, this is the over-wintering stage, from which the adults of the next generation will emerge the following year.
Male attraction tο virgin females in the almond seed wasp, Eurytomα amygdali Enderlein (Hymenoptera, Eurytomidae)
Authors: Pittara I.S. , Katsouannos Β.Ι.
Published: November 2017

Laboratory observations showed that adults of the almond seed wasp, Eurytoma amygdali Enderlein (Hymenoptera: Eurytomidae) were sexually mature on the first day of emergence. Experiments with an olfactometer showed that 1 to 12 day-old virgin females attracted the males. This attraction was found to be due to an odor, apparently a pheromone, released by the virgin females. The responsiveness of the males was higher the first part of the photophase. Under field conditions too, virgin females were attractive to males. “Delta” traps, containing 5-20 virgin females, suspended on almond trees, attracted and captured males released among the same trees. On the contrary, traps containing mated females or empty cages were not attractive to males.
Effect of size, color and height of pheromone baited sticky traps on captures of Dacus oleae flies
Authors: Haniotakis G.E.
Published: November 2017

The size of sticky pheromone traps has no effect on Dacus οleae catches when insect population densities are low. At high population densities trap catches increase with surface area. There were no differences in the numbers of D. οleae caught on yellow and white pheromone traps in periods of high pheromone effectiveness, which coincide with periods of high insect reproductive activity. During periods of reduced pheromone effectiveness, due either to low temperatures at the time of pheromone activity (dusk) or reduced insect reproductive activity, traps of yellow hues tend to capture more insects than traps of white color. The height at which pheromone traps are located inside the tree canopy has no effect on catches of D. οleae.
Α revision of tenuipalpid mites of Greece (Acari: Tenuipalpidae)
Authors: Hatzinikolis Ε.Ν.
Published: November 2017

The family Tenuipalpidae from Greece is revised and keys to the Greek species of the gene­ra Aegyptobia, Brevipalpus, Cenopalpus, Pentamerismus and Tenuipalpus are provided. A key to the Greek tenuipalpid genera is also given. The species: Aegyptobia leiahensis, Phytoptipaipusparadoxus, Brevipalpus recki, Pentamerismus coronatus, P. juniperi, P. o­regonensis, Pseudoleptus zelihae, Dolichotetranychus floridanus, Raoiella macfarlanei and Obdulia tamaricis are recorded for the first time. Two new species Aegyptobia karys­tensis and Aegyptobia aliartensis are described and illustrated. Hosts, distributional data and relation to hosts are presented for each species. A revaluation of the world genera and subgenera of the Tenuipalpidae is presented.
Drosophila schmidti Duda: redescription et iconographie des genitalia (Diptera, Drosophilidae)
Authors: Chassagnard Μ.Τ. , Tsacas L.
Published: November 2017

Drosophila schmidti Duda, n’a jamais été retrouvée depuis sa description. Les genitalia, structure diagnostique importante, n’ont jamais été iconographiês. Est donnée ici une re­description détaillée de l’espêw avec des dessins des genitalia et de l’ovipositeur. La posi­tion de cette espèce par rapport aux divers sous-genres du genre Drosophile est discutée.
Typhlodromus erymanthii, a new species of the family Phytoseiidae (Αcari: Mesostigmata) from Greece
Authors: Papadoulis ΤΗ. , Emmanouel Ν. G.
Published: November 2017

The adult female of Typhlodromus erymanthii, which belongs to the simplex species – group, is described and illustrated. It was collected from Quercus coccifera L. leaf erinea, and its association with other mites there is discussed. The dorsal setae, shield and leg setae were covered by a (waxy?) material.