bluuebunny/crossref_metadata_2025 huggingface dataset:
Forest Structure, Wood Standing Stock, and Tree Biomass in Different Restoration Systems in the Brazilian Atlantic Forest
Authors: Pontes Danila Morena Fidéles , Engel Vera Lex , Parrotta John A.
Published: July 2019

Reliable estimates of tree growth and wood yield are fundamental to support the management of restored forests and better reconcile the objectives of recovering biodiversity with the provision of ecosystem services. In this study, wood standing volumes and tree biomass stocks were estimated in different ecological restoration systems and at two sites with contrasting soil fertility, in order to evaluate the potential trade-offs between biodiversity and forest production. At each site, a complete randomized block design, with three replications of six treatments, was established in 1997–1998: direct seeding (DIRS), high-diversity tree plantation (HDIV), modified “Taungya” agroforestry system (AFS), mixed plantation with timber and firewood species (MIX), managed agroforestry system (AFSm) and managed mixed plantation (MIXm). We inventoried all trees with diameter at breast height (DBH) ≥ 5 cm in 450 m2 per treatment per plot, 19–20 years after establishment, using site-specific allometric models. Significant site effects were found for tree height, tree density and wood volume. Restoration systems (treatments) affected forest structure and forest productivity. Higher wood stock and biomass tree were observed in the less complex system (DIRS), while AFSm and HDIV reconciled higher species richness and diversity with good wood volume yields and tree biomass.
Shock Capturing in Large Eddy Simulations by Adaptive Filtering
Authors: Patel Sumit Kumar , Mathew Joseph
Published: July 2019

A method for shock capturing by adaptive filtering for use with high-resolution, high-order schemes for Large Eddy Simulations (LES) is presented. The LES method used in all the examples here employs the Explicit Filtering approach and the spatial derivatives are obtained with sixth-order, compact, finite differences. The adaptation is to drop the order of the explicit filter to two at gridpoints where a shock is detected, and to then increase the order from 2 to 10 in steps at successive gridpoints away from the shock. The method is found to be effective in a series of tests of common inviscid 1D and 2D problems of shock propagation and propagation of waves through shocks. As a prelude to LES, the 3D Taylor–Green problem for the inviscid and a finite viscosity case were simulated. An assessment of the overall performance of the method for LES was carried out by simulating an underexpanded round jet at a Reynolds number of 6.09 million, based in centerline velocity and diameter at nozzle exit plane. Very close quantitative agreement was found for the development of centerline mean pressure when compared to experiment. Simulations on several increasingly finer grids showed a monotonic extension of the computed part of the inertial range, with little change to low frequency content. Amplitudes and locations of large changes in pressure through several cells were captured accurately. A similar performance was observed for LES of an impinging jet containing normal and curved shocks.
Bioprocessed Production of Resveratrol-Enriched Rice Wine: Simultaneous Rice Wine Fermentation, Extraction, and Transformation of Piceid to Resveratrol from Polygonum cuspidatum Roots
Authors: Yang Kai-Ruei , Yu Hui-Chuan , Huang Chun-Yung , Kuo Jen-Min , Chang Cheng , Shieh Chwen-Jen , Kuo Chia-Hung
Published: July 2019

A new bioprocess to produce resveratrol-enriched rice wine was established and the effects of adding Polygonum cuspidatum root powder to rice wine fermentation were investigated. In this new process, piceid and resveratrol were extracted from P. cuspidatum roots to rice wine and piceid was converted to resveratrol by β-glucosidase during fermentation. After 10 days co-fermentation, rice wine with high levels of resveratrol was obtained, which contained ~14% (v/v) ethanol, 122 mg/L piceid, and 86 mg/L resveratrol. The resveratrol-enriched rice wine had enhanced antioxidant activity with significantly stronger 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric ion reducing power, and ferrous ion chelating capability. Ultrafiltration (UF) was employed in this study using hollow fibers to clarify the end product, increase shelf life without heat treatment, and maintain the quality of the phenolic compounds. The boiled and UF-treated rice wine were evaluated for ethanol, piceid, resveratrol, clarity, aerobic plate count, total acidity, pH, reducing sugars, and amino acids. The quality of the resveratrol-enriched rice wine was maintained after four weeks storage at normal refrigeration temperatures.
Hand-Feel Touch Cues and Their Influences on Consumer Perception and Behavior with Respect to Food Products: A Review
Authors: Pramudya Ragita C. , Seo Han-Seok
Published: July 2019

There has been a great deal of research investigating intrinsic/extrinsic cues and their influences on consumer perception and purchasing decisions at points of sale, product usage, and consumption. Consumers create expectations toward a food product through sensory information extracted from its surface (intrinsic cues) or packaging (extrinsic cues) at retail stores. Packaging is one of the important extrinsic cues that can modulate consumer perception, liking, and decision making of a product. For example, handling a product packaging during consumption, even just touching the packaging while opening or holding it during consumption, may result in a consumer expectation of the package content. Although hand-feel touch cues are an integral part of the food consumption experience, as can be observed in such an instance, little has been known about their influences on consumer perception, acceptability, and purchase behavior of food products. This review therefore provided a better understanding about hand-feel touch cues and their influences in the context of food and beverage experience with a focus on (1) an overview of touch as a sensory modality, (2) factors influencing hand-feel perception, (3) influences of hand-feel touch cues on the perception of other sensory modalities, and (4) the effects of hand-feel touch cues on emotional responses and purchase behavior.
The First Complete Mitochondrial Genome of the Flathead Cociella crocodilus (Scorpaeniformes: Platycephalidae) and the Phylogenetic Relationships within Scorpaeniformes Based on Whole Mitogenomes
Authors: Cui Lei , Cao Rongbo , Dong Yuelei , Gao Xingchen , Cen Jingyi , Lu Songhui
Published: July 2019

Complete mitochondrial genomes (mitogenomes) are important molecular markers for understanding the phylogenetics of various species. Although recent studies on the mitogenomes of the Scorpaeniformes species have been greatly advanced, information regarding molecular studies and the taxonomic localization of Platycephalidae is still sparse. To further analyze the phylogeny of Platycephalidae, we sequenced the complete mitogenome of Cociella crocodilus of the Platycephalidae family within Scorpaeniformes for the first time. The mitogenome was 17,314 bp in length, contained two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), 13 protein-coding genes (PCGs), and two typical noncoding control regions (the control region (CR) and origin of the light strand (OL)). All PCGs used standard initiation codons ATG, apart from cox1. The majority of the tRNA genes could be folded into cloverleaf secondary structures, whereas the secondary structure of tRNASer (AGN) lacked a dihydrouridine (DHU) stem. The CR contained several conserved sequence blocks (CSBs) and eight tandem repeats. In addition, the phylogenetic relationship based on the concatenated nucleotides sequences of 13 PCGs indicated that the Platycephalidae species are relatively basal in the phylogenetic relationships of Scorpaeniformes. Our results may not only advance the origin and the evolution of Scorpaeniformes, but also provide information for the genetic evolution and taxonomy of the teleostean species.
Ketogenic Diet and Microbiota: Friends or Enemies?
Authors: Paoli Antonio , Mancin Laura , Bianco Antonino , Thomas Ewan , Mota João Felipe , Piccini Fabio
Published: July 2019

Over the last years, a growing body of evidence suggests that gut microbial communities play a fundamental role in many aspects of human health and diseases. The gut microbiota is a very dynamic entity influenced by environment and nutritional behaviors. Considering the influence of such a microbial community on human health and its multiple mechanisms of action as the production of bioactive compounds, pathogens protection, energy homeostasis, nutrients metabolism and regulation of immunity, establishing the influences of different nutritional approach is of pivotal importance. The very low carbohydrate ketogenic diet is a very popular dietary approach used for different aims: from weight loss to neurological diseases. The aim of this review is to dissect the complex interactions between ketogenic diet and gut microbiota and how this large network may influence human health.
Altered in Vitro Metabolomic Response of the Human Microbiota to Sweeteners
Authors: Vamanu Emanuel , Pelinescu Diana , Gatea Florentina , Sârbu Ionela
Published: July 2019

Non-nutritive sweeteners represent an ingredient class that directly affects human health, via the development of inflammatory processes that promote chronic diseases related to microbiota dysbiosis. Several in vitro tests were conducted in the static GIS1 simulator. The aim of the study was to highlight the effect of sweeteners on the microbiota pattern of healthy individuals, associated with any alteration in the metabolomic response, through the production of organic acids and ammonium. The immediate effect of the in vitro treatment and the influence of the specific sweetener type on the occurrence of dysbiosis were evaluated by determining the biomarkers of the microbiota response. The presence of the steviol reduced the ammonium level (minimum of 410 mg/L), while the addition of cyclamate and saccharin caused a decrease in the number of microorganisms, in addition to lowering the total quantity of synthesized short-chain fatty acids (SCFAs). The bifidobacteria appeared to decrease below 102 genomes/mL in all the analyzed samples at the end of the in vitro simulation period. Barring the in vitro treatment of steviol, all the sweeteners tested exerted a negative influence on the fermentative profile, resulting in a decline in the fermentative processes, a rise in the colonic pH, and uniformity of the SCFA ratio.
Effect of LED Lighting and Gibberellic Acid Supplementation on Potted Ornamentals
Authors: Mills-Ibibofori Taylor , Dunn Bruce L. , Maness Niels , Payton Mark
Published: July 2019

Use of light emitting diode (LED) technology is beginning to replace traditional lighting in greenhouses. This research focused on the effects of LED lighting and gibberellic acid supplementation on growth and flowering of Dahlia spp. ‘Karma Serena’, Liatris spicata ‘Kobold’, and Lilium asiatic ‘Yellow Cocotte’. Light treatments, used to extend photoperiod, included LED flowering lamps and halogen lamps that emitted a combination of red + far-red + white, red + white, and broad spectrum from late fall to early spring. Gibberellic acid treatments ranged from 40 to 340 mg L−1 for Asiatic lily ‘Yellow Cocotte’, 50 to 250 for gayfeather ‘Kobold’, and 50 to 150 for dahlia ‘Karma Serena’. Results varied within species in response to light and gibberellic acid. A significant interaction of light with gibberellic acid influenced mean flower number and flowering percentage for dahlia ‘Karma Serena’, while flowering percentage and flower diameter were influenced for Asiatic lily ‘Yellow Cocotte’. Effect of light was most significant on growth and flowering measurements, especially for gayfeather ‘Kobold’ and dahlia ‘Karma Serena’. For gayfeather ‘Kobold’, flowering occurred two weeks earlier under sole LED lighting than under other light treatments and no supplemental light. Although flowering occurred the earliest for dahlia ‘Karma Serena’ under no supplemental light, plants under light treatments had greater height, width, and shoot weight. Significant effects of gibberellic acid on growth and flowering measurements for dahlia ‘Karma Serena’ and Asiatic lily ‘Yellow Cocotte’ were observed for height, width, and flower number.
Factors of Trust in Immediate Leaders: An Empirical Study in Police Service Environment
Authors: Tomaževič Nina , Aristovnik Aleksander
Published: July 2019

The study has two objectives—first, to examine the dimensionality of police service employees’ job satisfaction and their assessment of the enablers from the Common Assessment Framework, analyzing these facets at different organizational levels, and second, to identify the impact of selected facets of job satisfaction on trust in one’s immediate leader. The study is based on a comprehensive on-line questionnaire where 1209 responses from police service employees were acquired, and for the data analysis, factor analysis was first used to formulate the factors of job satisfaction facets and of the Common Assessment Framework enablers. Second, structural equation modelling was performed to identify the correlations of the studied variables. The Common Assessment Framework enabler Strategy/Leadership was significantly correlated with the enabler Processes and had an indirect impact on Trust through direct impacts on Leadership style in the organizational unit and Autonomy/Tasks. Both of the latter determinants had a significant influence on Trust in one’s immediate leader. The confirmed the impact of determinants from different hierarchical levels indicates the importance of efforts by managers at all organizational levels should the police management intend to achieve and maintain high levels of trust in one’s immediate leader in police service work environment.
The Power of Radio to Promote Health and Resilience in Natural Disasters: A Review
Authors: Hugelius Karin , Adams Mike , Romo-Murphy Eila
Published: July 2019

Humanitarian radio has been used in humanitarian aid efforts and after natural disasters over the last 15 years. However, the effects have barely been evaluated, and there are few scientific reports on the impact of radio as a disaster health response intervention. Therefore, this study aimed to provide an overview of the use and impact of humanitarian radio in natural disasters from a health perspective. A literature review of 13 scientific papers and grey literature resources was conducted. The results show that humanitarian radio could be used to promote both physical and psychosocial wellbeing by providing health-related information, advice and psychosocial support in natural disasters. Community resilience can be enhanced by the promotion of community engagement and can strengthen self-efficacy and community efficacy. Radio also has the potential to cost-effectively reach a large number of affected people in areas with severely damaged infrastructure. Radio could, therefore, contribute to health recovery and wellbeing from both individual and community perspectives. As such, health professionals; crises communication professionals, including radio journalists; and disaster-managing stakeholders should be prepared and trained to use humanitarian radio as an integrated part of the disaster health response in natural disasters.
Effect of Contextualized Versus Non-Contextualized Interventions for Improving Hand Washing, Sanitation, and Health in Rural Tanzania: Study Design of a Cluster Randomized Controlled Trial
Authors: Dockx Kim , Van Remoortel Hans , De Buck Emmy , Schelstraete Charlotte , Vanderheyden An , Lievens Tiene , Kinyagu John Thomas , Mamuya Simon , Vandekerckhove Philippe
Published: July 2019

Nearly 90% of diarrhea-related mortalities are the result of unsafe drinking water, poor sanitation, and insufficient hygiene. Although “Water, Sanitation, and Hygiene” (WASH) interventions may significantly reduce the risk of diarrheal disease, it is currently unclear which interventions are the most effective. In this study, we aim to determine the importance of contextualizing a WASH intervention to the local context and the needs for increasing impact (Clinicaltrials.gov NCT03709368). A total of 1500 households in rural Tanzania will participate in this cluster randomized controlled trial. Households will be randomized into one of three cohorts: (1) a control group receiving a basic intervention and 1 placebo household visit, (2) an intervention group receiving a basic intervention + 9 additional household visits which are contextualized to the setting using the RANAS approach, and (3) an intervention group receiving a basic intervention + 9 additional household visits, which are not contextualized, i.e., a general intervention. Assessments will take place at a baseline, 1 and 2 years after the start of the intervention, and 1 year after the completion of the intervention. Measurements involve questionnaires and spot checks. The primary outcome is hand-washing behavior, secondary objectives include, the impact on latrine use, health, WASH infrastructure, quality of life, and cost-effectiveness.
Collision Detection for UAVs Based on GeoSOT-3D Grids
Authors: Zhai Weixin , Tong Xiaochong , Miao Shuangxi , Cheng Chengqi , Ren Fuhu
Published: July 2019

The increasing number of unmanned aerial vehicles (UAVs) has led to challenges related to solving the collision problem to ensure air traffic safety. The traditional approaches employed for collision detection suffer from two main drawbacks: first, the computational burden of a pairwise calculation increases exponentially with an increasing number of spatial entities; second, existing grid-based approaches are unsuitable for complicated scenarios with a large number of objects moving at high speeds. In the proposed model, we first identified UAVs and other spatial objects with GeoSOT-3D grids. Second, the nonrelational spatial database was initialized with a multitable strategy, and spatiotemporal data were inserted with the GeoSOT-3D grid codes as the primary key. Third, the collision detection procedure was transformed from a pairwise calculation to a multilevel query. Four simulation experiments were conducted to verify the feasibility and efficiency of the proposed collision detection model for UAVs in different environments. The results also indicated that 64 m GeoSOT-3D grids are the most suitable basic grid size, and the reduction in the time consumption compared with traditional methods reached approximately 50–80% in different scenarios.
Application of the Scorpion Neurotoxin AaIT against Insect Pests
Authors: Deng Sheng-Qun , Chen Jia-Ting , Li Wen-Wen , Chen Min , Peng Hong-Juan
Published: July 2019

Androctonus australis Hector insect toxin (AaIT), an insect-selective toxin, was identified in the venom of the scorpion Androctonus australis. The exclusive and specific target of the toxin is the voltage-gated sodium channels of the insect, resulting in fast excitatory paralysis and even death. Because of its strict toxic selectivity and high bioactivity, AaIT has been widely used in experiments exploring pest bio-control. Recombinant expression of AaIT in a baculovirus or a fungus can increase their virulence to insect pests and diseases vectors. Likewise, transgenic plants expressing AaIT have notable anti-insect activity. AaIT is an efficient toxin and has great potential to be used in the development of commercial insecticides.
Discovering and Constructing ceRNA-miRNA-Target Gene Regulatory Networks during Anther Development in Maize
Authors: Li Ziwen , An Xueli , Zhu Taotao , Yan Tingwei , Wu Suowei , Tian Youhui , Li Jinping , Wan Xiangyuan
Published: July 2019

The “competing endogenous RNA (ceRNA) hypothesis” has recently been proposed for a new type of gene regulatory model in many organisms. Anther development is a crucial biological process in plant reproduction, and its gene regulatory network (GRN) has been gradually revealed during the past two decades. However, it is still unknown whether ceRNAs contribute to anther development and sexual reproduction in plants. We performed RNA and small RNA sequencing of anther tissues sampled at three developmental stages in two maize lines. A total of 28,233 stably transcribed loci, 61 known and 51 potentially novel microRNAs (miRNAs) were identified from the transcriptomes. Predicted ceRNAs and target genes were found to conserve in sequences of recognition sites where their corresponding miRNAs bound. We then reconstructed 79 ceRNA-miRNA-target gene regulatory networks consisting of 51 known miRNAs, 28 potentially novel miRNAs, 619 ceRNA-miRNA pairs, and 869 miRNA-target gene pairs. More than half of the regulation pairs showed significant negative correlations at transcriptional levels. Several well-studied miRNA-target gene pairs associated with plant flower development were located in some networks, including miR156-SPL, miR159-MYB, miR160-ARF, miR164-NAC, miR172-AP2, and miR319-TCP pairs. Six target genes in the networks were found to be orthologs of functionally confirmed genes participating in anther development in plants. Our results provide an insight that the ceRNA-miRNA-target gene regulatory networks likely contribute to anther development in maize. Further functional studies on a number of ceRNAs, miRNAs, and target genes will facilitate our deep understanding on mechanisms of anther development and sexual plants reproduction.
Metal Complexes of Oxadiazole Ligands: An Overview
Authors: Salassa Giovanni , Terenzi Alessio
Published: July 2019

Oxadizoles are heterocyclic ring systems that find application in different scientific disciplines, from medicinal chemistry to optoelectronics. Coordination with metals (especially the transition ones) proved to enhance the intrinsic characteristics of these organic ligands and many metal complexes of oxadiazoles showed attractive characteristics for different research fields. In this review, we provide a general overview on different metal complexes and polymers containing oxadiazole moieties, reporting the principal synthetic approaches adopted for their preparation and showing the variety of applications they found in the last 40 years.
Extraction and Analysis of Compounds with Antibacterial Potential from the Red Alga Grateloupia turuturu
Authors: Cardoso Isabel , Cotas João , Rodrigues Ana , Ferreira Daniel , Osório Nádia , Pereira Leonel
Published: July 2019

Nowadays, the development of new drugs only relies on a small number of molecules and 50% of all existent drugs are extracted or synthetically obtained. This work intends to evaluate the antibacterial potential of the ethanolic and polysaccharide extracts obtained from Grateloupia turuturu and to characterize the composition of the alga’s polysaccharides by FTIR-ATR. We used sequential extraction to obtain the extracts that were tested against S. aureus and E. coli. The ethanolic extracts in E. coli, at the highest concentration used (15 mg mL−1) showed 45.7% (Tetrasporophyte extract) and 55.1% (Carposporophyte extract) of growth reduction and in S. aureus 56.2% (T extract) and 51.8% (C extract). Polysaccharide extracts started showing significant reduction effect on E. coli and S. aureus growth at 7.5 mg mL−1 with a reduction of 54.9% and 39.5%, respectively. At 15 mg mL−1 the reduction observed was 88.5% and 85.4%. The FTIR-ATR allowed to characterize G. turuturu’s polysaccharides concluding that it is composed by a hybrid kappa/iota carrageenan with traces of agar, in both phases of the life cycle. This work allows us to conclude about the antibacterial properties of this alga and the compounds that might be behind this activity, showing that there’s a lot more than a small number of molecules that can be used as natural drugs.
Structural Differences Influence Biological Properties of Glucosylceramides from Clinical and Environmental Isolates of Scedosporium aurantiacum and Pseudallescheria minutispora
Authors: Caneppa Adriana , de Meirelles Jardel , Rollin-Pinheiro Rodrigo , Xisto Mariana , Liporagi-Lopes Livia , de Souza Lauro , Villela Romanos Maria , Barreto-Bergter Eliana
Published: July 2019

Scedosporium/Lomentospora complex is composed of filamentous fungi, including some clinically relevant species, such as Pseudallescheria boydii, Scedosporium aurantiacum, and Scedosporium apiospermum. Glucosylceramide (GlcCer), a conserved neutral glycosphingolipid, has been described as an important cell surface molecule playing a role in fungal morphological transition and pathogenesis. The present work aimed at the evaluation of GlcCer structures in S. aurantiacum and Pseudallescheria minutispora, a clinical and an environmental isolate, respectively, in order to determine their participation in fungal growth and host-pathogen interactions. Structural analysis by positive ion-mode ESI-MS (electrospray ionization mass spectrometer) revealed the presence of different ceramide moieties in GlcCer in these species. Monoclonal antibodies against Aspergillus fumigatus GlcCer could recognize S. aurantiacum and P. minutispora conidia, suggesting a conserved epitope in fungal GlcCer. In addition, these antibodies reduced fungal viability, enhanced conidia phagocytosis by macrophages, and decreased fungal survival inside phagocytic cells. Purified GlcCer from both species led to macrophage activation, increasing cell viability as well as nitric oxide and superoxide production in different proportions between the two species. These results evidenced some important properties of GlcCer from species of the Scedosporium/Lomentospora complex, as well as the effects of monoclonal anti-GlcCer antibodies on fungal cells and host-pathogen interaction. The differences between the two species regarding the observed biological properties suggest that variation in GlcCer structures and strain origin could interfere in the role of GlcCer in host-pathogen interaction.
Lexical Crosslinguistic Influence and Study Abroad: Do Learners Use L1-Based Resources Less?
Authors: Ortega Mireia , Celaya M. Luz
Published: July 2019

Research in Crosslinguistic Influence (CLI) has traditionally addressed two broad types of lexical CLI—transfer of form and transfer of meaning (Ringbom 1987)—which were reconceptualized by Jarvis (2009) as lexemic and lemmatic transfer, respectively. Whereas the former considers the phonological and graphemic structure of words, the latter is related to semantic and syntactic properties. These types of lexical CLI have been analysed in relation to L2 proficiency, but not in relation to factors such as Study Abroad (SA), which the present study aims to investigate. The oral production by 107 Catalan/Spanish learners of English as a Foreign Language (EFL) was analysed in terms of lexical CLI and the amount of input received during their SA. Results show an inverse relationship between the amount of input in SA and lexical CLI; that is, the higher the number of hours abroad, the fewer cases of lexical CLI. Statistical differences were found for lemmatic CLI and for one type of lexemic CLI. In light of these findings, it is suggested that learners that take part in SA programmes do not rely on L1-based resources when gaps in their knowledge arise.
The Role of Language Policy Documents in the Internationalisation of Multilingual Higher Education: An Exploratory Corpus-Based Study
Authors: Villares Rosana
Published: July 2019

Using corpus methods, this study explores the role of Language Policy (LP) documents in the internationalisation process of Spanish universities. It aims at understanding how non-Anglophone universities integrate English and local languages in the functions of education, research, and administration. Content analysis was used for the identification of key themes, and discourse analysis examined how those themes were textually expressed. Consistent with previous literature, this study shows that relevant strategic areas of LP deal with training, regulation, accreditation, and support measures. Results also highlight the role played by institutions in LP and the presence of language hierarchies between English and local languages. The discussion of these findings leads to further inquiry of mismatches between top-down institutional expectations and bottom-up realities regarding the design and implementation of institutional policies.
Non Smooth Contact Dynamics Approach for Mechanical Systems Subjected to Friction-Induced Vibration
Authors: Sinou Jean-Jacques , Chiello Olivier , Charroyer Lucien
Published: July 2019

The modeling of contact is one of the main features of contact dynamics in the context of friction-induced vibrations. It can have a strong impact on the numerical results and consequently on the design choices during the optimization or specification of industrial mechanical systems. This is particularly the case for scientific studies interested in brake squeal. The objective of the paper is to recall and to promote developments concerning the use of non smooth contact dynamics approach for numerical simulations based on finite element method. The specific problem of the prediction of self-excited vibration in the context of brake squeal is discussed. In order to illustrate the potential benefit for the mechanical community of using formulations and theoretical developments from the mathematical community, the stability analysis and the estimation of nonlinear vibrations of a brake system with multiple frictional interface is investigated.
A Methodology to Systematically Investigate the Diffusion Degradation of Cemented Carbide during Machining of a Titanium Alloy
Authors: Saketi Sara , Östby Jonas , Bexell Ulf , Olsson Mikael
Published: July 2019

Using Ti6Al4V as a work material, a methodology to systematically investigate the diffusion degradation of cemented carbide during machining is proposed. The methodology includes surface characterization of as-tested worn inserts, wet etched worn inserts, metallographic cross-sectioned worn inserts as well as the back-side of the produced chips. Characterization techniques used include scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), Auger electron spectroscopy (AES) and time of flight secondary ion mass spectroscopy (ToF-SIMS). The results show that the characterization of wet etched worn inserts gives quick and useful information regarding the diffusion degradation of cemented carbide, in the present work the formation of a fine crystalline W layer (carbon depleted WC layer) at the tool-work material interface. The present study also illuminates the potential of AES analysis when it comes to analyzing the degradation of cemented carbide in contact with the work material during machining. The high surface sensitivity in combination with high lateral resolution makes it possible to analyze the worn cemented carbide surface on a sub-µm level. Especially AES sputter depth profiling, resulting in detailed information of variations in chemical composition across interfaces, is a powerful tool when it comes to understanding diffusion wear. Finally, the present work illustrates the importance of analyzing not only the worn tool but also the produced chips. An accurate characterization of the back-side of the chips will give important information regarding the wear mechanisms taking place at the tool rake face–chip interface. Surface analysis techniques such as AES and ToF-SIMS are well suited for this type of surface characterization.
Graph-Based Image Matching for Indoor Localization
Authors: Manzo Mario
Published: July 2019

Graphs are a very useful framework for representing information. In general, these data structures are used in different application domains where data of interest are described in terms of local and spatial relations. In this context, the aim is to propose an alternative graph-based image representation. An image is encoded by a Region Adjacency Graph (RAG), based on Multicolored Neighborhood (MCN) clustering. This representation is integrated into a Content-Based Image Retrieval (CBIR) system, designed for the vision-based positioning task. The image matching phase, in the CBIR system, is managed with an approach of attributed graph matching, named the extended-VF algorithm. Evaluated in a context of indoor localization, the proposed system reports remarkable performance.
The Quantitative Analysis of Workers’ Stress Due to Working Environment in the Production System of the Automobile Part Manufacturing Industry
Authors: Omair Muhammad , Ullah Misbah , Ganguly Baishakhi , Noor Sahar , Maqsood Shahid , Sarkar Biswajit
Published: July 2019

Production now requires the management of production processes and operations on the basis of customers’ demand to ensure the best combination of technology and humans in the system. The role of the humans in the production process is very significant for the production and quality of the product. The production system depends upon technology and human factors and is highly influenced by the working conditions of the workers, that is, work load, physical, dealings, job timings and so forth. In the current global economy, minimizing production costs is a serious priority for the industries. However, the costs of bad working conditions increase the intensity of the average stress among employees to cause extra costs by affecting the workers’ efficiency and products’ quality, which is invisible in the eyes of decision makers. This research identifies the cost of workers’ stress by developing a linkage between the economic benefits of the firms and the social upgrading of the workers. A numerical example of a production based system is performed to represent the real-time application of the proposed model. A sensitivity analysis is also carried out to quantify the impact of average stress among workers on the production system. Sequential quadratic programming is used to optimize the given nonlinear model for production planning. The optimal results influence ergonomics awareness and the relationship with the safety culture among managers in a firm. It is concluded that efficient and effective production cannot be possible without considering the working conditions of humans in the firm. Managerial insights are also generated from the implications of the results and sensitivity analysis.
Cantor Paradoxes, Possible Worlds and Set Theory
Authors: Usó-Doménech José-Luis , Nescolarde-Selva Josué-Antonio , Segura-Abad Lorena , Gash Hugh , Alonso-Stenberg Kristian
Published: July 2019

In this paper, we illustrate the paradox concerning maximally consistent sets of propositions, which is contrary to set theory. It has been shown that Cantor paradoxes do not offer particular advantages for any modal theories. The paradox is therefore not a specific difficulty for modal concepts, and it also neither grants advantages nor disadvantages for any modal theory. The underlying problem is quite general, and affects anyone who intends to use the notion of “world” in its ontology.
On-Chip Integration of Pressure Plus 2-Axis (X/Z) Acceleration Composite TPMS Sensors with a Single-Sided Bulk-Micromachining Technique
Authors: Wang Jiachou , Song Fang
Published: July 2019

A novel on-chip integration of pressure plus 2-axis (X/Z) acceleration composite sensors for upgraded production of automobile tire pressure monitoring system (TPMS) is proposed, developed, and characterized. Herein, the X-axis accelerometer is with the cantilever beam-mass structure and is used for automatically identifying and positioning each of the four wheels. The IC-Foundry-Compatible low-cost batch fabrication technique of MIS (i.e., Micro-openings Inter-etch and Sealing) is employed to only fabricate the device from the front side of (111) silicon wafer, without double-sided micromachining, wafer bonding, complex Cavity-SOI (Silicon on Insulator) processing, and expensive SOI-wafer needed. Benefited from the single-wafer front-side fabrication technique on ordinary single-polished wafers, the fabricated composite TPMS sensor has the advantages of a small chip-size of 1.9 mm × 1.9 mm, low cross-talk interference, low-cost, and compatible process with IC-foundries. The fabricated pressure sensors, X-axis accelerometer and Z-axis accelerometer, show linear sensing outputs, with the sensitivities as about 0.102 mV/kPa, 0.132 mV/kPa, and 0.136 mV/kPa, respectively. Fabricated with the low-cost front-side MIS process, the fabricated composite TPMS sensors are promising in automotive electronics and volume production.
Editorial for Special Issue “Polymetallic Metallogenic System”
Authors: Yang Liqiang
Published: July 2019

In the last century, following the development of Earth System Science, the metallogenic system has become an important topic in the study of mineral deposits [...]
A UV/Vis Spectroscopy-Based Assay for Monitoring of Transformations Between Nucleosides and Nucleobases
Authors: Kaspar Felix , Giessmann Robert T. , Krausch Niels , Neubauer Peter , Wagner Anke , Gimpel Matthias
Published: July 2019

Efficient reaction monitoring is crucial for data acquisition in kinetic and mechanistic studies. However, for conversions of nucleosides to their corresponding nucleobases, as observed in enzymatically catalyzed nucleoside phosphorylation reactions, the current analytical arsenal does not meet modern requirements regarding cost, speed of analysis and high throughput. Herein, we present a UV/Vis spectroscopy-based assay employing an algorithm for spectral unmixing in a 96-well plate format. The algorithm relies on fitting of reference spectra of nucleosides and their bases to experimental spectra and allows determination of nucleoside/nucleobase ratios in solution with high precision. The experimental procedure includes appropriate dilution of a sample into aqueous alkaline solution, transfer to a multi-well plate, measurement of a UV/Vis spectrum and subsequent in silico spectral unmixing. This enables data collection in a high-throughput fashion and reduces costs compared to state-of-the-art HPLC analyses by approximately 5-fold while being 20-fold faster and offering comparable precision. Additionally, the method is robust regarding dilution and sample transfer errors as it only considers spectral form and not absolute intensity. It can be applied to all natural nucleosides and nucleobases and even unnatural ones as demonstrated by several examples.
Biological Responses of Onion-Shaped Carbon Nanoparticles
Authors: Jang Jaehee , Kim Youngjun , Hwang Jangsun , Choi Yonghyun , Tanaka Masayoshi , Kang Eunah , Choi Jonghoon
Published: July 2019

Nanodiamonds are emerging as new nanoscale materials because of their chemical stability, excellent crystallinity, and unique optical properties. In this study, the structure of nanodiamonds was engineered to produce carbon nano-onion particles (CNOs) with multiple layers. Following a series of physicochemical characterizations of the CNOs, various evaluations for biological responses were conducted for potential biotechnological applications of the CNOs. The possibility of biological applications was first confirmed by assessment of toxicity to animal cells, evaluation of hemolysis reactions, and evaluation of reactive oxygen species. In addition, human immune cells were evaluated for any possible induction of an immune response by CNOs. Finally, the toxicity of CNOs to Escherichia coli present in the human colon was evaluated. CNOs have the chemical and physical properties to be a unique variety of carbon nanomaterials, and their toxicity to animal and human cells is sufficiently low that their biotechnological applications in the future are expected.
Assessment of the Dose–Response Relationship between Meal Protein Content and Postprandial Thermogenesis: Effect of Sex and the Oral Contraceptive Pill
Authors: Duhita Maharani R. , Schutz Yves , Montani Jean-Pierre , Dulloo Abdul G. , Miles-Chan Jennifer L.
Published: July 2019

Implementation of efficacious dietary interventions to regulate energy balance requires understanding of the determinants of individual response. To date, information regarding individual variability in response to elevated meal protein content is lacking. This study investigates whether sex and/or oral contraceptive pill (OCP) use play a role in the response to elevated meal protein in 21 healthy young adults (seven men, seven women not taking OCP, and seven women who were OCP users). Participants consumed each of three standardized isocaloric (590 kcal) meals of differing protein content (11, 23, 31% kcal protein). Resting energy expenditure (EE), respiratory quotient (RQ), hunger and satiety were measured at baseline (fasting) and during 180 min postprandial. Whilst significant dose–response increases in EE were observed in men, meal protein-induced EE in women without OCP reached a maximum at <23% protein. Women taking OCP reported lower postprandial fullness than women without OCP, despite similar body size, but also, most notably, no significant difference in EE response between any of the meals. Whilst the mechanisms underpinning this thermogenic inflexibility in response across a wide-range (three-fold) of protein meal content require further investigation, this highlights the need for careful consideration of factors that may influence an individual’s metabolic response to dietary interventions aimed at optimising postprandial thermogenesis for body weight regulation.
The Preventive Effects of Greenshell Mussel (Perna canaliculus) on Early-Stage Metabolic Osteoarthritis in Rats with Diet-Induced Obesity
Authors: Siriarchavatana Parkpoom , Kruger Marlena C. , Miller Matthew R. , Tian Hong Sabrina , Wolber Frances M.
Published: July 2019

The prevalence of osteoarthritis (OA) is rising worldwide, with the most pronounced increase being in the category of metabolic-associated osteoarthritis (MetOA). This is predicted to worsen with the global rise in aging societies and obesity. To address this health burden, research is being conducted to identify foods that can reduce the incidence or severity of MetOA. Oil from the Greenshell mussel (Perna canaliculus) (GSM), a native New Zealand shellfish, has been successfully used to reduce OA symptoms. The current study assessed the effect of including flash-dried powder from whole GSM meat as part of a normal (control) versus high-fat/high-sugar (HFHS) diet for 13 weeks on the development of MetOA in rats. Rats fed a HFHS diet developed metabolic dysregulation and obesity with elevated plasma leptin and HbA1C concentrations. Visible damage to knee joint cartilage was minimal, but plasma levels of C telopeptide of type II collagen (CTX-II), a biomarker of cartilage degradation, were markedly higher in HFHS-fed rats compared to control-fed rats. However, rats fed the HFHS diet containing GSM had significantly reduced serum CTX-II. Inclusion of GSM in rats fed the control diet also lowered CTX-II. These findings suggest that dietary GSM can reduce the incidence or slow the progression of early MetOA.
Western Diet-Induced Metabolic Alterations Affect Circulating Markers of Liver Function before the Development of Steatosis
Authors: Gabbia Daniela , Roverso Marco , Guido Maria , Sacchi Diana , Scaffidi Michela , Carrara Maria , Orso Genny , Russo Francesco Paolo , Floreani Annarosa , Bogialli Sara , De Martin Sara
Published: July 2019

Since nutrition might have a significant impact on liver function, we analyzed the early effect of Western-type diet on hepatic tissue and lipid and drug metabolism in Wistar–Kyoto rats (n = 8); eight rats fed with a standard diet were used as controls. Histological analysis of liver tissue was performed, and plasma biochemical parameters were measured. Plasma concentration of six bile acids was determined by ultra-liquid chromatography-tandem mass spectrometry UHPLC-MS/MS. Hepatic gene expressions of enzymes involved in drug and lipid metabolism were assessed by means of real-time reverse transcription (qRT)-PCR. Liver of rats fed with a Western diet did not show macroscopic histological alterations, but number and diameter of lipid droplets increased, as well as DGAT1, GPAT4, SCD, FASN and SREBP2 expression. Furthermore, Western diet-fed animals showed an increase in the activation of hepatic stellate cells and macrophage number in liver tissue, as well as a significant increase in AST and bilirubin levels (p < 0.01), and in the LDL:HDL cholesterol ratio (p < 0.001). Plasma chenodeoxycholic acid concentration increased significantly, whereas cholic acid decreased (p < 0.05), and cytochrome P450 genes were generally downregulated. Significant changes in hepatic lipid and drug metabolism are early induced by the Western diet, prior to steatosis development. Such changes are associated with a peculiar alteration in circulating bile acids, which could represent an early marker of non-alcoholic fatty liver disease (NAFLD) development.
Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study
Authors: Santiago-López Lourdes , Hernández-Mendoza Adrián , Vallejo-Cordoba Belinda , Mata-Haro Verónica , Wall-Medrano Abraham , González-Córdova Aarón F.
Published: July 2019

The aim of this study was to evaluate the effect of milk fermented with Lactobacillus fermentum J20 (FMJ20) or J28 (FMJ28) on ameliorating indomethacin-induced inflammation. Twenty-eight male C57Bl/6 mice were divided into four experimental groups: indomethacin, indomethacin + FMJ20, indomethacin + FMJ28, and untreated (control). Groups were fed fermented milk for 15 days, followed by administration of indomethacin supplied in three sub-doses over experimental period. Body weight, and food consumption were recorded. Additionally, spleen, kidney, and liver were weighed, and the small intestine length was measured. The cytokines in serum (IL-2, IL-4, IL-6, IL-10, IL-17, IL-23 and TNFα) and in intestinal mucosa (IL-17 and IFNγ) were also determined. Compared to the control, all indomethacin-supplemented groups lost weight (~2.7 g; p < 0.05), but no changes were found in the organ-specific morphometry analysis. FMJ28 showed better results in attenuating serum and intestinal IL-17 levels. Furthermore, showed less epithelial cell loss and inflammatory infiltrates than the other indomethacin-treated groups. These results suggest that FMJ28 may be effective in reducing intestinal and systemic acute inflammation, specifically in mice.
Quantitative Prediction of Human Pharmacokinetics and Pharmacodynamics of CKD519, a Potent Inhibitor of Cholesteryl Ester Transfer Protein (CETP)
Authors: Choi Suein , Han Seunghoon , Jeon Sangil , Yim Dong-Seok
Published: July 2019

CKD519, a selective inhibitor of cholesteryl ester transfer protein(CETP), is undergoing development as an oral agent for the treatment of primary hypercholesterolemia and mixed hyperlipidemia. The aim of this study was to predict the appropriate efficacious dose of CKD519 for humans in terms of the inhibition of CETP activity by developing a CKD519 pharmacokinetic/pharmacodynamic (PK/PD) model based on data from preclinical studies. CKD519 was intravenously and orally administered to hamsters, rats, and monkeys for PK assessment. Animal PK models of all dose levels in each species were developed using mixed effect modeling analysis for exploration, and an interspecies model where allometric scaling was applied was developed based on the integrated animal PK data to predict the human PK profile. PD parameters and profile were predicted using in vitro potency and same-in-class drug information. The two-compartment first-order elimination model with Weibull-type absorption and bioavailability following the sigmoid Emax model was selected as the final PK model. The PK/PD model was developed by linking the interspecies PK model with the Emax model of the same-in-class drug. The predicted PK/PD profile and parameters were used to simulate the human PK/PD profiles for different dose levels, and based on the simulation result, the appropriate efficacious dose was estimated as 25 mg in a 60 kg human. However, there were some discrepancies between the predicted and observed human PK/PD profiles compared to the phase I clinical data. The huge difference between the observed and predicted bioavailability suggests that there is a hurdle in predicting the absorption parameter only from animal PK data.