bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Probiotics on Small Intestinal Microbiota Composition, Inflammatory Cytokines and Intestinal Permeability in Patients with Non-Alcoholic Fatty Liver Disease
Authors: Ayob Nurainina , Muhammad Nawawi Khairul Najmi , Mohamad Nor Mohamad Hizami , Raja Ali Raja Affendi , Ahmad Hajar Fauzan , Oon Seok Fang , Mohd Mokhtar Norfilza
Published: February 2023

The prevalence of non-alcoholic fatty liver disease (NAFLD) has soared globally. As our understanding of the disease grows, the role of the gut-liver axis (GLA) in NAFLD pathophysiology becomes more apparent. Hence, we focused mainly on the small intestinal area to explore the role of GLA. We looked at how multi-strain probiotics (MCP® BCMC® strains) containing six different Lactobacillus and Bifidobacterium species affected the small intestinal gut microbiota, inflammatory cytokines, and permeability in NAFLD patients. After six months of supplementation, biochemical blood analysis did not show any discernible alterations in either group. Five predominant phyla known as Actinobacteria, Proteobacteria, Firmicutes, Bacteroidota and Fusobacteria were found in NAFLD patients. The probiotics group demonstrated a significant cluster formation of microbiota composition through beta-diversity analysis (p < 0.05). This group significantly reduced three unclassifiable species: unclassified_Proteobacteria, unclassified_Streptococcus, and unclassified_Stenotrophomonas. In contrast, the placebo group showed a significant increase in Prevotella_melaninogenica and Rothia_mucilaginosa, which were classified as pathogens. Real-time quantitative PCR analysis of small intestinal mucosal inflammatory cytokines revealed a significant decrease in IFN-γ (−7.9 ± 0.44, p < 0.0001) and TNF-α (−0.96 ± 0.25, p < 0.0033) in the probiotics group but an increase in IL-6 (12.79 ± 2.24, p < 0.0001). In terms of small intestinal permeability analysis, the probiotics group, unfortunately, did not show any positive changes through ELISA analysis. Both probiotics and placebo groups exhibited a significant increase in the level of circulating zonulin (probiotics: 107.6 ng/mL ± 124.7, p = 0.005 vs. placebo: 106.9 ng/mL ± 101.3, p = 0.0002) and a significant decrease in circulating zonula occluden-1 (ZO-1) (probiotics: −34.51 ng/mL ± 18.38, p < 0.0001 vs. placebo: −33.34 ng/mL ± 16.62, p = 0.0001). The consumption of Lactobacillus and Bifidobacterium suggested the presence of a well-balanced gut microbiota composition. Probiotic supplementation improves dysbiosis in NAFLD patients. This eventually stabilised the expression of inflammatory cytokines and mucosal immune function. To summarise, more research on probiotic supplementation as a supplement to a healthy diet and lifestyle is required to address NAFLD and its underlying causes.
Mechanistic Modelling of DNA Damage Repair by the Radiation Adaptive Response Mechanism and Its Significance
Authors: Piotrowski Łukasz , Krasowska Julianna , Fornalski Krzysztof W.
Published: February 2023

The radiation adaptive response effect is a biophysical phenomenon responsible for the enhancement of repair processes in irradiated cells. This can be observed in dedicated radiobiological experiments, e.g., where the small priming dose of ionising radiation is given before the high challenging one (the so-called Raper–Yonezawa effect). The situation is more complicated when the whole complex system (the organism) is taken into consideration; many other mechanisms make the adaptive response weaker and—in some cases—practically insignificant. The recently published simplified Monte Carlo model of human lymphocytes irradiation by X-rays allows for the calculation of the level of repair enhancement by the adaptive response when every other cellular biological mechanism is implemented. The qualitative results show that the adaptive response phenomenon, observed with some probability on a basic level, usually blurs among other effects and becomes weaker than expected. Regardless, the radiation adaptive response is still an important biophysical effect which needs to be taken into consideration in low-dose radiobiological studies.
Difunctional Hydrogel Optical Fiber Fluorescence Sensor for Continuous and Simultaneous Monitoring of Glucose and pH
Authors: Li Yangjie , Luo Site , Gui Yongqiang , Wang Xin , Tian Ziyuan , Yu Haihu
Published: February 2023

It is significant for people with diabetes to know their body’s real-time glucose level, which can guide the diagnosis and treatment. Therefore, it is necessary to research continuous glucose monitoring (CGM) as it gives us real-time information about our health condition and its dynamic changes. Here, we report a novel hydrogel optical fiber fluorescence sensor segmentally functionalized with fluorescein derivative and CdTe QDs/3-APBA, which can continuously monitor pH and glucose simultaneously. In the glucose detection section, the complexation of PBA and glucose will expand the local hydrogel and decrease the fluorescence of the quantum dots. The fluorescence can be transmitted to the detector by the hydrogel optical fiber in real time. As the complexation reaction and the swelling–deswelling of the hydrogel are all reversible, the dynamic change of glucose concentration can be monitored. For pH detection, the fluorescein attached to another segment of the hydrogel exhibits different protolytic forms when pH changes and the fluorescence changes correspondingly. The significance of pH detection is compensation for pH errors in glucose detection because the reaction between PBA and glucose is sensitive to pH. The emission peaks of the two detection units are 517 nm and 594 nm, respectively, so there is no signal interference between them. The sensor can continuously monitor glucose in 0–20 mM and pH in 5.4–7.8. The advantages of this sensor are multi-parameter simultaneous detection, transmission-detection integration, real-time dynamic detection, and good biocompatibility.
Microfluidic Wound-Healing Assay for ECM and Microenvironment Properties on Microglia BV2 Cells Migration
Authors: Yazdanpanah Moghadam Ehsan , Sonenberg Nahum , Packirisamy Muthukumaran
Published: February 2023

Microglia cells, as the resident immune cells of the central nervous system (CNS), are highly motile and migratory in development and pathophysiological conditions. During their migration, microglia cells interact with their surroundings based on the various physical and chemical properties in the brain. Herein, a microfluidic wound-healing chip is developed to investigate microglial BV2 cell migration on the substrates coated with extracellular matrixes (ECMs) and substrates usually used for bio-applications on cell migration. In order to generate the cell-free space (wound), gravity was utilized as a driving force to flow the trypsin with the device. It was shown that, despite the scratch assay, the cell-free area was created without removing the extracellular matrix coating (fibronectin) using the microfluidic assay. It was found that the substrates coated with Poly-L-Lysine (PLL) and gelatin stimulated microglial BV2 migration, while collagen and fibronectin coatings had an inhibitory effect compared to the control conditions (uncoated glass substrate). In addition, the results showed that the polystyrene substrate induced higher cell migration than the PDMS and glass substrates. The microfluidic migration assay provides an in vitro microenvironment closer to in vivo conditions for further understanding the microglia migration mechanism in the brain, where the environment properties change under homeostatic and pathological conditions.
A CRISPR-Cas and Tat Peptide with Fluorescent RNA Aptamer System for Signal Amplification in RNA Imaging
Authors: Tang Heng , Peng Junran , Jiang Xin , Peng Shuang , Wang Fang , Weng Xiaocheng , Zhou Xiang
Published: February 2023

We reported on an efficient RNA imaging strategy based on a CRISPR-Cas and Tat peptide with a fluorescent RNA aptamer (TRAP-tag). Using modified CRISPR-Cas RNA hairpin binding proteins fused with a Tat peptide array that recruits modified RNA aptamers, this simple and sensitive strategy is capable of visualizing endogenous RNA in cells with high precision and efficiency. In addition, the modular design of the CRISPR-TRAP-tag facilitates the substitution of sgRNAs, RNA hairpin binding proteins, and aptamers in order to optimize imaging quality and live cell affinity. With CRISPR-TRAP-tag, exogenous GCN4, endogenous mRNA MUC4, and lncRNA SatIII were distinctly visualized in single live cells.
Molecularly Imprinted Polymer-Based Luminescent Chemosensors
Authors: Liu Ruoyang , Ko Chi-Chiu
Published: February 2023

Molecularly imprinted polymer (MIP)-based luminescent chemosensors combine the advantages of the highly specific molecular recognition of the imprinting sites and the high sensitivity with the luminescence detection. These advantages have drawn great attention during the past two decades. Luminescent molecularly imprinted polymers (luminescent MIPs) towards different targeted analytes are constructed with different strategies, such as the incorporation of luminescent functional monomers, physical entrapment, covalent attachment of luminescent signaling elements on the MIPs, and surface-imprinting polymerization on the luminescent nanomaterials. In this review, we will discuss the design strategies and sensing approaches of luminescent MIP-based chemosensors, as well as their selected applications in biosensing, bioimaging, food safety, and clinical diagnosis. The limitations and prospects for the future development of MIP-based luminescent chemosensors will also be discussed.
Bioluminescent RIPoptosome Assay for FADD/RIPK1 Interaction Based on Split Luciferase Assay in a Human Neuroblastoma Cell Line SH-SY5Y
Authors: Ghanavatian Parisa , Salehi-Sedeh Hossein , Ataei Farangis , Hosseinkhani Saman
Published: February 2023

Different programed cell death (PCD) modalities involve protein–protein interactions in large complexes. Tumor necrosis factor α (TNFα) stimulated assembly of receptor-interacting protein kinase 1 (RIPK1)/Fas-associated death domain (FADD) interaction forms Ripoptosome complex that may cause either apoptosis or necroptosis. The present study addresses the interaction of RIPK1 and FADD in TNFα signaling by fusion of C-terminal (CLuc) and N-terminal (NLuc) luciferase fragments to RIPK1-CLuc (R1C) or FADD-NLuc (FN) in a caspase 8 negative neuroblastic SH-SY5Y cell line, respectively. In addition, based on our findings, an RIPK1 mutant (R1C K612R) had less interaction with FN, resulting in increasing cell viability. Moreover, presence of a caspase inhibitor (zVAD.fmk) increases luciferase activity compared to Smac mimetic BV6 (B), TNFα -induced (T) and non-induced cell. Furthermore, etoposide decreased luciferase activity, but dexamethasone was not effective in SH-SY5Y. This reporter assay might be used to evaluate basic aspects of this interaction as well as for screening of necroptosis and apoptosis targeting drugs with potential therapeutic application.
Comparison of In-Person and Online Recordings in the Clinical Teleassessment of Speech Production: A Pilot Study
Authors: Python Grégoire , Demierre Cyrielle , Bourqui Marion , Bourbon Angelina , Chardenon Estelle , Trouville Roland , Laganaro Marina , Fougeron Cécile
Published: February 2023

In certain circumstances, speech and language therapy is proposed in telepractice as a practical alternative to in-person services. However, little is known about the minimum quality requirements of recordings in the teleassessment of motor speech disorders (MSD) utilizing validated tools. The aim here is to examine the comparability of offline analyses based on speech samples acquired from three sources: (1) in-person recordings with high quality material, serving as the baseline/gold standard; (2) in-person recordings with standard equipment; (3) online recordings from videoconferencing. Speech samples were recorded simultaneously from these three sources in fifteen neurotypical speakers performing a screening battery of MSD and analyzed by three speech and language therapists. Intersource and interrater agreements were estimated with intraclass correlation coefficients on seventeen perceptual and acoustic parameters. While the interrater agreement was excellent for most speech parameters, especially on high quality in-person recordings, it decreased in online recordings. The intersource agreement was excellent for speech rate and mean fundamental frequency measures when comparing high quality in-person recordings to the other conditions. The intersource agreement was poor for voice parameters, but also for perceptual measures of intelligibility and articulation. Clinicians who plan to teleassess MSD should adapt their recording setting to the parameters they want to reliably interpret.
Combining Transcranial Magnetic Stimulation and Deep Brain Stimulation: Current Knowledge, Relevance and Future Perspectives
Authors: D’Onofrio Valentina , Manzo Nicoletta , Guerra Andrea , Landi Andrea , Baro Valentina , Määttä Sara , Weis Luca , Porcaro Camillo , Corbetta Maurizio , Antonini Angelo , Ferreri Florinda
Published: February 2023

Deep brain stimulation (DBS) has emerged as an invasive neuromodulation technique for the treatment of several neurological disorders, but the mechanisms underlying its effects remain partially elusive. In this context, the application of Transcranial Magnetic Stimulation (TMS) in patients treated with DBS represents an intriguing approach to investigate the neurophysiology of cortico-basal networks. Experimental studies combining TMS and DBS that have been performed so far have mainly aimed to evaluate the effects of DBS on the cerebral cortex and thus to provide insights into DBS’s mechanisms of action. The modulation of cortical excitability and plasticity by DBS is emerging as a potential contributor to its therapeutic effects. Moreover, pairing DBS and TMS stimuli could represent a method to induce cortical synaptic plasticity, the therapeutic potential of which is still unexplored. Furthermore, the advent of new DBS technologies and novel treatment targets will present new research opportunities and prospects to investigate brain networks. However, the application of the combined TMS-DBS approach is currently limited by safety concerns. In this review, we sought to present an overview of studies performed by combining TMS and DBS in neurological disorders, as well as available evidence and recommendations on the safety of their combination. Additionally, we outline perspectives for future research by highlighting knowledge gaps and possible novel applications of this approach.
Intracerebral Electrophysiological Recordings to Understand the Neural Basis of Human Face Recognition
Authors: Rossion Bruno , Jacques Corentin , Jonas Jacques
Published: February 2023

Understanding how the human brain recognizes faces is a primary scientific goal in cognitive neuroscience. Given the limitations of the monkey model of human face recognition, a key approach in this endeavor is the recording of electrophysiological activity with electrodes implanted inside the brain of human epileptic patients. However, this approach faces a number of challenges that must be overcome for meaningful scientific knowledge to emerge. Here we synthesize a 10 year research program combining the recording of intracerebral activity (StereoElectroEncephaloGraphy, SEEG) in the ventral occipito-temporal cortex (VOTC) of large samples of participants and fast periodic visual stimulation (FPVS), to objectively define, quantify, and characterize the neural basis of human face recognition. These large-scale studies reconcile the wide distribution of neural face recognition activity with its (right) hemispheric and regional specialization and extend face-selectivity to anterior regions of the VOTC, including the ventral anterior temporal lobe (VATL) typically affected by magnetic susceptibility artifacts in functional magnetic resonance imaging (fMRI). Clear spatial dissociations in category-selectivity between faces and other meaningful stimuli such as landmarks (houses, medial VOTC regions) or written words (left lateralized VOTC) are found, confirming and extending neuroimaging observations while supporting the validity of the clinical population tested to inform about normal brain function. The recognition of face identity – arguably the ultimate form of recognition for the human brain – beyond mere differences in physical features is essentially supported by selective populations of neurons in the right inferior occipital gyrus and the lateral portion of the middle and anterior fusiform gyrus. In addition, low-frequency and high-frequency broadband iEEG signals of face recognition appear to be largely concordant in the human association cortex. We conclude by outlining the challenges of this research program to understand the neural basis of human face recognition in the next 10 years.
Parasomnias in Pregnancy
Authors: Bušková Jitka , Miletínová Eva , Králová Radana , Dvořáková Tereza , Tefr Faridová Adéla , Heřman Hynek , Hrdličková Kristýna , Šebela Antonín
Published: February 2023

Objectives: Pregnancy is often associated with reduced sleep quality and an increase in sleep disorders, such as restless leg syndrome, obstructive sleep apnea, and insomnia. There are few studies investigating the prevalence of parasomnias in pregnancy, although they may be expected to be a significant problem, as disturbed sleep in this time period in addition to these sleep disorders may trigger parasomnia episodes. Methods: We conducted a survey using an online questionnaire focusing on a comparison of the prevalence of parasomnias in three time periods: 3 months before pregnancy, during pregnancy, and 3 months after delivery. We also inquired about psychiatric and neurological comorbidities, current anxiety and depression symptoms, and pregnancy complications. Results: A total of 325 women (mean age 30.3 ± 5.3 years) participated in the online survey. The overall number of reported parasomnias increased during pregnancy compared to the 3 months before pregnancy (p < 0.001) and decreased after childbirth (p < 0.001). Specifically, we found a significant increase in sleepwalking (p = 0.02) and night terrors (p < 0.001), as well as in vivid dreams (p < 0.001) and nightmares (p < 0.001) during pregnancy. A similar significant increase during pregnancy was reported for head explosion (p < 0.011). In contrast, the number of episodes of sleep paralysis increased after delivery (p = 0.008). At the individual level, an increase in the severity/frequency of individual parasomnia episodes was also observed during pregnancy. Participants whose vivid dreams/nightmares persisted after delivery had higher BDI-II and STAI-T scores. Our data also suggest a significant impact of migraines and other chronic pain, as well as complications during pregnancy, on the presence of parasomnia episodes in our cohort. Conclusions: We have shown that the prevalence of parasomnias increases during pregnancy and needs to be targeted, especially by non-pharmacological approaches. At the same time, it is necessary to inquire about psychiatric and neurological comorbidities and keep in mind that more sleep disorders may be experienced by mothers who have medical complications during pregnancy.
The Curious Case of Impersonators and Singers: Telling Voices Apart and Telling Voices Together under Naturally Challenging Listening Conditions
Authors: Stevenage Sarah V. , Singh Lucy , Dixey Pru
Published: February 2023

Vocal identity processing depends on the ability to tell apart two instances of different speakers whilst also being able to tell together two instances of the same speaker. Whilst previous research has examined these voice processing capabilities under relatively common listening conditions, it has not yet tested the limits of these capabilities. Here, two studies are presented that employ challenging listening tasks to determine just how good we are at these voice processing tasks. In Experiment 1, 54 university students were asked to distinguish between very similar sounding, yet different speakers (celebrity targets and their impersonators). Participants completed a ‘Same/Different’ task and a ‘Which is the Celebrity?’ task to pairs of speakers, and a ‘Real or Not?’ task to individual speakers. In Experiment 2, a separate group of 40 university students was asked to pair very different sounding instances of the same speakers (speaking and singing). Participants were presented with an array of voice clips and completed a ‘Pairs Task’ as a variant of the more traditional voice sorting task. The results of Experiment 1 suggested that significantly more mistakes were made when distinguishing celebrity targets from their impersonators than when distinguishing the same targets from control voices. Nevertheless, listeners were significantly better than chance in all three tasks despite the challenge. Similarly, the results of Experiment 2 suggested that it was significantly more difficult to pair singing and speaking clips than to pair two speaking clips, particularly when the speakers were unfamiliar. Again, however, the performance was significantly above zero, and was again better than chance in a cautious comparison. Taken together, the results suggest that vocal identity processing is a highly adaptable task, assisted by familiarity with the speaker. However, the fact that performance remained above chance in all tasks suggests that we had not reached the limit of our listeners’ capability, despite the considerable listening challenges introduced. We conclude that voice processing is far better than previous research might have presumed.
Based on Tau PET Radiomics Analysis for the Classification of Alzheimer’s Disease and Mild Cognitive Impairment
Authors: Jiao Fangyang , Wang Min , Sun Xiaoming , Ju Zizhao , Lu Jiaying , Wang Luyao , Jiang Jiehui , Zuo Chuantao
Published: February 2023

Alzheimer’s Disease (AD) and Mild Cognitive Impairment (MCI) are closely associated with Tau proteins accumulation. In this study, we aimed to implement radiomics analysis to discover high-order features from pathological biomarker and improve the classification accuracy based on Tau PET images. Two cross-racial independent cohorts from the ADNI database (121 AD patients, 197 MCI patients and 211 normal control (NC) subjects) and Huashan hospital (44 AD patients, 33 MCI patients and 36 NC subjects) were enrolled. The radiomics features of Tau PET imaging of AD related brain regions were computed for classification using a support vector machine (SVM) model. The radiomics model was trained and validated in the ADNI cohort and tested in the Huashan hospital cohort. The standard uptake value ratio (SUVR) and clinical scores model were also performed to compared with radiomics analysis. Additionally, we explored the possibility of using Tau PET radiomics features as a good biomarker to make binary identification of Tau-negative MCI versus Tau-positive MCI or apolipoprotein E (ApoE) ε4 carrier versus ApoE ε4 non-carrier. We found that the radiomics model demonstrated best classification performance in differentiating AD/MCI patients and NC in comparison to SUVR and clinical scores models, with an accuracy of 84.8 ± 4.5%, 73.1 ± 3.6% in the ANDI cohort. Moreover, the radiomics model also demonstrated greater performance in diagnosing AD than other methods in the Huashan hospital cohort, with an accuracy of 81.9 ± 6.1%. In addition, the radiomics model also showed the satisfactory classification performance in the MCI-tau subgroup experiment (72.3 ± 3.5%, 71.9 ± 3.6% and 63.7 ± 5.9%) and in the MCI-ApoE subgroup experiment (73.5 ± 4.3%, 70.1 ± 3.9% and 62.5 ± 5.4%). In conclusion, our study showed that based on Tau PET radiomics analysis has the potential to guide and facilitate clinical diagnosis, further providing evidence for identifying the risk factors in MCI patients.
Paper Planes for Teaching Construction Production Systems Based on Lean Tools: Continuous Improvement Cells and 5S
Authors: Sánchez Omar , Revuelta María P. , Gómez-Cabrera Adriana , Salazar Luis A.
Published: February 2023

Teaching production systems and Lean tools is a challenge for educators in the construction area, which is highlighted by the difficulty of emulating in the classroom the scenarios that industry professionals will face. Hence, implementing pedagogical games has a high potential to improve construction education processes. However, gaps are observed in the proposal of pedagogical games applicable to teaching construction systems based on Lean tools. Considering this gap, this paper proposes a game of paper planes to support the teaching of production systems in construction based on the Lean tools Continuous Improvement Cells and 5S. The research method consisted of applying the Design Science Research (DSR) method to develop, evaluate, and improve the game proposal. Thus, the game was assumed as an artifact subject to a development and improvement process to solve an identified problem. The proposed game consists of three main rounds: (1) production system design, (2) Continuous Improvement Cells implementation, and (3) 5S implementation. The main theoretical and practical contribution is the proposal of a pedagogical game to support the teaching of construction production systems based on Lean tools, evaluating students’ knowledge by applying surveys before and after the game and assessing performance indicators. The application of the game to a group of undergraduate and graduate students showed a broad positive impact on the assimilation of the principles of construction production systems based on Lean tools.
Responses of Structural Components of a Full-Scale Nailed Retaining Structure under the Influence of Surcharge Loading and Nail Head Configuration: A Numerical Study
Authors: Gui Meen-Wah , Rajak Ravendra P.
Published: February 2023

Soil-nailing is a simple and economical method of stabilizing cut slopes and retaining excavation. Most of the soil-nailing related studies, in particular the experimental work, were conducted in idealized or homogeneous ground, but such a result might not necessarily be representative. Thus, for a more representative study, instead of treating the ground as homogeneous it should be treated as a system of horizontal layers. This study assessed the performance of a full-scale nailed retaining structure for a foundation pit of a 20-storey building through a series of numerical analyses. The influence of full-face facing thickness, nail head geometrical configuration (size and thickness) and surcharge loading on the response of the structural components of the soil-nailing system adopted is the main concern. The results were evaluated in terms of axial force, shear force and bending moment of the structural facing element and the horizontal displacement of the soil retained behind the facing element. In both cases, the distribution of nail axial (tensile) force in each nail reinforcement was also compared and evaluated. It was found that the thickness of full-face facing affected the facing shear force and bending moment, while the surcharge loading influenced the facing axial force and the horizontal displacement of the retained soil and that the magnitude of the axial force registered at the fixed end was governed by the size of the discrete nail head.
Optimal Insulation Assessment, Emission Analysis, and Correlation Formulation for Indian Region
Authors: Kallioğlu Mehmet Ali , Yılmaz Ahmet , Sharma Ashutosh , Mohamed Ahmed , Dobrotă Dan , Alam Tabish , Khargotra Rohit , Singh Tej
Published: February 2023

The current study depicts the effects of different insulation materials and fuel types on the cooling and heating performance of buildings situated in hot and dry, warm and humid, composite, and cold climatic conditions in India. Ten different locations chosen from diverse climatic regions were selected, and various potential parameters for expanded polystyrene and extruded polystyrene insulation materials were evaluated. Potential parameters, such as optimal insulation thickness, annual savings, and payback period, were computed for cooling and heating requirements and were found in the ranges of 0.0428–0.891 m, 10.83–19.19 $/m2, and 1.49–2.36 years for cooling, as well as 0.0063–0.1522 m, 0.29–55.92 $/m2, and 0.95–6.52 years for heating, respectively. An emission analysis was also carried out for the estimation of greenhouse gas (GHG) emissions by the engagement of optimal insulation thickness for heating. The GHG emissions from natural gas, coal, and diesel by the employment of various insulating materials were found in the ranges of 5.39–11.28, 9.47–32.68, and 2.26–4.51 kg/m2-year, respectively. A correlation formulation (power) for optimal insulation thickness was also carried out. For checking the preciseness of the developed mathematical models, statistical tools were utilized, and their obtained values in the satisfactory range signified the accurateness of the developed models.
Biomass-Derived N-Doped Activated Carbon from Eucalyptus Leaves as an Efficient Supercapacitor Electrode Material
Authors: Bejjanki Dinesh , Banothu Praveen , Kumar Vijay Bhooshan , Kumar Puttapati Sampath
Published: February 2023

Biomass-derived activated carbon is one of the promising electrode materials in supercapacitor applications. In this work bio-waste (oil extracted from eucalyptus leaves) was used as a carbon precursor to synthesize carbon material with ZnCl2 as a chemical activating agent and activated carbon was synthesized at various temperatures ranging from 400 to 800 °C. The activated carbon at 700 °C showed a surface area of 1027 m2 g−1 and a specific capacitance of 196 F g−1. In order to enhance the performance, activated carbon was doped with nitrogen-rich urea at a temperature of 700 °C. The obtained activated carbon and N-doped activated carbon was characterized by phase and crystal structural using (XRD and Raman), morphological using (SEM), and compositional analysis using (FTIR). The electrochemical measurements of carbon samples were evaluated using an electrochemical instrument and NAC-700 °C exhibited a specific capacitance of 258 F g−1 at a scan rate of 5 mV s−1 with a surface area of 1042 m2 g−1. Thus, surface area and functionalizing the groups with nitrogen showed better performance and it can be used as an electrode material for supercapacitor cell applications.
Implications of an Underlying Beckwith–Wiedemann Syndrome for Wilms Tumor Treatment Strategies
Authors: Quarello Paola , Carli Diana , Biasoni Davide , Gerocarni Nappo Simona , Morosi Carlo , Cotti Roberta , Garelli Emanuela , Zucchetti Giulia , Spadea Manuela , Tirtei Elisa , Spreafico Filippo , Fagioli Franca
Published: February 2023

Beckwith–Wiedemann Syndrome (BWS) is a pediatric overgrowth disorder involving a predisposition to embryonal tumors. Most of the tumors associated with BWS occur in the first 8–10 years of life, and the most common is Wilms tumor (WT). BWS clinical heterogeneity includes subtle overgrowth features or even silent phenotypes, and WT may be the presenting symptom of BWS. WT in BWS individuals exhibit distinct characteristics from those of sporadic WT, and the management of these patients needs a peculiar approach. The most important feature is a higher risk of developing bilateral disease at some time in the course of the illness (synchronous bilateral disease at diagnosis or metachronous recurrence after initial presentation with unilateral disease). Accordingly, neoadjuvant chemotherapy is the recommended approach also for BWS patients with unilateral WT to facilitate nephron-sparing surgical approaches. This review emphasizes the importance of early BWS recognition, particularly if a WT has already occurred, as this will result in an urgent consideration of first-line cancer therapy.
Ferroptosis Inducers Kill Mesenchymal Stem Cells Affected by Neuroblastoma
Authors: Li Xiangze , Wang Qi , Xu Chencheng , Zhang Lei , Zhou Jiquan , Lv Jingchun , Xu Min , Jiang Dapeng
Published: February 2023

Bone marrow (BM) is the most common site of neuroblastoma (NB) metastasis, and its involvement represents poor patient prognosis. In accordance with the “seed and soil” theory of tumor metastasis, BM provides a favorable environment for NB metastasis while bone marrow mesenchymal stem cells (BMSCs) have been recognized as a central part of tumor stroma formation. Yet, there is currently no effective method for intervening these BMSCs. We found that BMSCs affected by NB (NB-BMSCs) could significantly promote NB growth and migration. Additionally, tumor cell-endowed BMSCs showed stronger resistance to several chemotherapeutic agents. Surprisingly, NB-BMSCs were more sensitive to ferroptosis than normal BMSCs. NB-BMSCs had lower levels of intracellular free iron while synthesizing more iron-sulfur clusters and heme. Moreover, the Xc−/glutathione/glutathione peroxidase 4 (Xc−/GSH/GPX4) pathway of the anti-ferroptosis system was significantly downregulated. Accordingly, ferroptosis inducers erastin and RAS-selective lethal 3 (RSL3) could significantly kill NB-BMSCs with limited effects on normal BMSCs. BMSCs from NB patients with BM metastasis also showed poor anti-ferroptosis ability compared with those from NB patients without BM metastasis. In vivo studies suggested that co-injection of mice with BMSCs and NB cells could significantly promote the growth of tumor tissues compared with injecting NB cells alone. However, treatment with erastin or RSL3 resulted in the opposite effect to some extent. Our results revealed that NB-BMSCs were vulnerable to ferroptosis from downregulation of the Xc−/GSH/GPX4 pathway. Ferroptosis inducers could effectively kill NB-BMSCs, but not normal BMSCs. This study provides possible new ideas for the treatment of tumor-associated BMSCs in NB patients.
A Simple 3D Cell Culture Method for Studying the Interactions between Human Mesenchymal Stromal/Stem Cells and Patients Derived Glioblastoma
Authors: Oliver Lisa , Álvarez-Arenas Arturo , Salaud Céline , Jiménez-Sanchez Juan , Calvo Gabriel F. , Belmonte-Beitia Juan , Blandin Stephanie , Vidal Luciano , Pérez Victor , Heymann Dominique , Vallette François M.
Published: February 2023

We have developed a 3D biosphere model using patient-derived cells (PDCs) from glioblastoma (GBM), the major form of primary brain tumors in adult, plus cancer-activated fibroblasts (CAFs), obtained by culturing mesenchymal stem cells with GBM conditioned media. The effect of MSC/CAFs on the proliferation, cell-cell interactions, and response to treatment of PDCs was evaluated. Proliferation in the presence of CAFs was statistically lower but the spheroids formed within the 3D-biosphere were larger. A treatment for 5 days with Temozolomide (TMZ) and irradiation, the standard therapy for GBM, had a marked effect on cell number in monocultures compared to co-cultures and influenced cancer stem cells composition, similar to that observed in GBM patients. Mathematical analyses of spheroids growth and morphology confirm the similarity with GBM patients. We, thus, provide a simple and reproducible method to obtain 3D cultures from patient-derived biopsies and co-cultures with MSC with a near 100% success. This method provides the basis for relevant in vitro functional models for a better comprehension of the role of tumor microenvironment and, for precision and/or personalized medicine, potentially to predict the response to treatments for each GBM patient.
Management of Hepatocellular Carcinoma in Decompensated Cirrhotic Patients: A Comprehensive Overview
Authors: Tampaki Maria , Papatheodoridis George V. , Cholongitas Evangelos
Published: February 2023

Primary liver cancer is the sixth most common cancer and the fourth leading cause of cancer-related death. Hepatocellular carcinoma (HCC) accounts for 75% of primary liver cancer cases, mostly on the basis of cirrhosis. However, the data and therapeutic options for the treatment of HCC in patients with decompensated cirrhosis are rather limited. This patient category is often considered to be in a terminal stage without the possibility of a specific treatment except liver transplantation, which is restricted by several criteria and liver donor shortages. Systemic treatments may provide a solution for patients with Child Pugh class B or C since they are less invasive. Although most of the existing trials have excluded patients with decompensated cirrhosis, there are increasing data from real-life settings that show acceptable tolerability and satisfying efficacy in terms of response. The data on the administration of locoregional treatments in such patients are also limited, but the overall survival seems to be potentially prolonged when patients are carefully selected, and close adverse event monitoring is applied. The aim of this review is to analyze the existing data regarding the administration of treatments in decompensated patients with HCC, evaluate the effect of therapy on overall survival and highlight the potential risks in terms of tolerability.
Serum Response Factor-Regulated IDO1/Kyn-Ahr Pathway Promotes Tumorigenesis of Oral Squamous Cell Carcinoma
Authors: Xu Mingyan , Zhu Feixiang , Yin Qi , Yin Hao , Fang Shaobin , Luo Gongwei , Huang Jie , Huang Wenxia , Liu Fan , Zhong Ming , Deng Xiaoling
Published: February 2023

Serum response factor (SRF) regulates pro-carcinogenic genes in various cancers, but its role in oral squamous cell carcinoma (OSCC) remains unclear. SRF expression in 70 OSCC samples was detected via immunohistochemistry. Abundant SRF expressed in OSCC tissues was closely associated with tumor metastasis. SRF-overexpressing OSCC cells were constructed to evaluate how SRF affects OSCC cell tumorigenesis and epithelial-to-mesenchymal transition (EMT) in vitro and in vivo. Overexpressed SRF increased OSCC cell migration and invasion in vitro and tumor growth and invasion in vivo. This promoted EMT, characterized by decreased and increased expression of E- and N-cadherin, respectively. Furthermore, an analysis of RNA sequences of transcriptional targets of SRF showed that SRF transactivated the indoleamine 2, 3-dioxygenase 1 (IDO1)/kynurenine-aryl hydrocarbon receptor (Kyn-AhR) signaling pathway in OSCC cell lines. Direct SRF binding to the IDO1 gene promoter upregulated transcription, which was detected through chromatin immunoprecipitation and dual luciferase reporter assays. Inhibiting IDO1 or AhR impaired SRF-induced migration and invasion and prevented EMT in OSCC cells. Our results demonstrated that SRF is a critical regulator of the IDO1/Kyn-AhR signaling pathway. This in turn increases OSCC cell migration and invasion by modulating EMT, which, consequently, favors OSCC cell growth and metastasis. We revealed a novel molecular mechanism through which SRF modulates OSCC metastasis. This should provide potential targets or biomarkers for OSCC diagnosis and treatment.
See Lung Cancer with an AI
Authors: Bidzińska Joanna , Szurowska Edyta
Published: February 2023

A lot has happened in the field of lung cancer screening in recent months. The ongoing discussion and documentation published by the scientific community and policymakers are of great importance to the entire European community and perhaps beyond. Lung cancer is the main worldwide killer. Low-dose computed tomography-based screening, together with smoking cessation, is the only tool to fight lung cancer, as it has already been proven in the United States of America but also European randomized controlled trials. Screening requires a lot of well-organized specialized work, but it can be supported by artificial intelligence (AI). Here we discuss whether and how to use AI for patients, radiologists, pulmonologists, thoracic surgeons, and all hospital staff supporting screening process benefits.
Approaches to Clinical Complete Response after Neoadjuvant Chemotherapy in Muscle-Invasive Bladder Cancer: Possibilities and Limitations
Authors: Lee Hye Won , Kwon Whi-An , Nguyen La Ngoc Thu , Phan Do Thanh Truc , Seo Ho Kyung
Published: February 2023

In the surgical oncology field, the change from a past radical surgery to an organ preserving surgery is a big trend. In muscle-invasive bladder cancer treatment, neoadjuvant chemotherapy (NAC) followed by radical cystectomy (RC) is the standard of care for muscle-invasive bladder cancer (MIBC) patients eligible for cisplatin. There is a growing interest in bladder preserving strategies after NAC because good oncologic outcome has been reported for pathologic complete response (pCR) patients after NAC, and many studies have continued to discuss whether bladder preservation treatment is possible for these patients. However, in actual clinical practice, decision-making should be determined according to clinical staging and there is a gap that cannot be ignored between clinical complete response (cCR) and pCR. Currently, there is a lack in a uniform approach to post-NAC restaging of MIBC and a standardized cCR definition. In this review, we clarify the gap between cCR and pCR at the current situation and focus on emerging strategies in bladder preservation in selected patients with MIBC who achieve cCR following NAC.
Photocatalytic Degradation of Paracetamol under Simulated Sunlight by Four TiO2 Commercial Powders: An Insight into the Performance of Two Sub-Micrometric Anatase and Rutile Powders and a Nanometric Brookite Powder
Authors: Blangetti Nicola , Freyria Francesca S. , Calviello Maria Chiara , Ditaranto Nicoletta , Guastella Salvatore , Bonelli Barbara
Published: February 2023

The photocatalytic degradation of the emerging contaminant paracetamol in aqueous solution has been studied under 1 SUN (~1000 W m−2) in the presence of four commercial TiO2 powders, namely sub-micrometric anatase and rutile, and nanometric brookite and P25 (the popular anatase/rutile mixture used as a benchmark in most papers). The rutile powder showed low activity, whereas, interestingly, the anatase and the brookite powders outperformed P25 in terms of total paracetamol conversion to carboxylic acids, which, according to the literature, are the final products of its degradation. To explain such results, the physicochemical properties of the powders were studied by applying a multi-technique approach. Among the physicochemical properties usually affecting the photocatalytic performance of TiO2, the presence of some surface impurities likely deriving from K3PO4 (used as crystallization agent) was found to significantly affect the percentage of paracetamol degradation obtained with the sub-micrometric anatase powder. To confirm the role of phosphate, a sample of anatase, obtained by a lab synthesis procedure and having a “clean” surface, was used as a control, though characterized by nanometric particles and higher surface area. The sample was less active than the commercial anatase, but it was more active after impregnation with K3PO4. Conversely, the presence of Cl at the surface of the rutile did not sizably affect the (overall poor) photocatalytic activity of the powder. The remarkable photocatalytic activity of the brookite nanometric powder was ascribed to a combination of several physicochemical properties, including its band structure and nanoparticles size.
Gold Complexes with Hydrophilic N-Heterocyclic Carbene Ligands and Their Contribution to Aqueous-Phase Catalysis
Authors: Crochet Pascale , Cadierno Victorio
Published: February 2023

N-Heterocyclic carbenes (NHCs) are nowadays one of the most widely employed ligands in organometallic chemistry and homogeneous catalysis due to the inherent stability of the metal-carbene bond and the ease of modification of the backbone as well as the N-wingtips substituents of these ligands. The functionalization of NHCs with hydrophilic groups offers the possibility of using NHC-metal complexes in aqueous catalysis, a hot topic within the Green Chemistry context due to the positive implications associated with the use of water as a reaction medium. In line with the enormous interest aroused by gold complexes in catalysis, significant efforts have been directed in the last years to the design and application of hydrophilic NHC-gold catalysts. This review is aimed to summarize the research in this area. The catalytic applications of water-soluble gold nanoparticles stabilized by hydrophilic NHCs are also covered.
Ni2P-Modified P-Doped Graphitic Carbon Nitride Hetero-Nanostructures for Efficient Photocatalytic Aqueous Cr(VI) Reduction
Authors: Andreou Evangelos K. , Koutsouroubi Eirini D. , Vamvasakis Ioannis , Armatas Gerasimos S.
Published: February 2023

Targeting heterostructures with modulated electronic structures and efficient charge carrier separation and mobility is an effective strategy to improve photocatalytic performance. In this study, we report the synthesis of 2D/3D hybrid heterostructures comprising P-doped graphitic carbon nitride (g-C3N4) nanosheets (ca. 50–60 nm in lateral size) and small-sized Ni2P nanoparticles (ca. 10–12 nm in diameter) and demonstrate their prominent activity in the photocatalytic reduction of Cr(VI). Utilizing a combination of spectroscopic and electrochemical characterization techniques, we unveil the reasons behind the distinct photochemical performance of these materials. We show that Ni2P modification and P doping of the g-C3N4 effectively improve the charge-carrier transportation and spatial separation through the interface of Ni2P/P-doped g-C3N4 junctions. As a result, the catalyst containing 15 wt.% Ni2P exhibits superior photocatalytic activity in the detoxification of Cr(VI)-contaminated effluents under UV-visible light illumination, presenting an apparent quantum yield (QY) of 12.5% at 410 nm, notably without the use of sacrificial additives. This study marks a forward step in understanding and fabricating cost-effective photocatalysts for photochemical applications.
Magnetic Nanomaterials as Catalysts for Syngas Production and Conversion
Authors: Chidhambaram Natarajan , Kay Samuel Jasmine Jecintha , Priyadharshini Saravanan , Meenakshi Rajakantham , Sakthivel Pandurengan , Dhanbalan Shanmugasundar , Shanavas Shajahan , Kamaraj Sathish-Kumar , Thirumurugan Arun
Published: February 2023

The conversion of diverse non-petroleum carbon elements, such as coal, biomass, natural/shale gas, and even CO2, into cleaner hydrocarbon fuels and useful chemicals relies heavily on syngas, which is a combination of CO and H2. Syngas conversions, which have been around for almost a century, will probably become even more important in the production of energy and chemicals due to the rising need for liquid fuels and chemical components derived from sources of carbon other than crude oil. Although a number of syngas-based technologies, including the production of methanol, Fischer–Tropsch (FT) synthesis, and carbonylation, have been industrialized, there is still a great need for new catalysts with enhanced activity and adjustable product selectivity. New novel materials or different combinations of materials have been investigated to utilize the synergistic effect of these materials in an effective way. Magnetic materials are among the materials with magnetic properties, which provide them with extra physical characteristics compared to other carbon-based or conventional materials. Moreover, the separation of magnetic materials after the completion of a specific application could be easily performed with a magnetic separation process. In this review, we discuss the synthesis processes of various magnetic nanomaterials and their composites, which could be utilized as catalysts for syngas production and conversion. It is reported that applying an external magnetic field could influence the outcomes of any applications of magnetic nanomaterials. Here, the possible influence of the magnetic characteristics of magnetic nanomaterials with an external magnetic field is also discussed.