bluuebunny/crossref_metadata_2025 huggingface dataset:
Animal Welfare and Mountain Products from Traditional Dairy Farms: How Do Consumers Perceive Complexity?
Authors: Zuliani Anna , Esbjerg Lars , Grunert Klaus , Bovolenta Stefano
Published: November 2018

This qualitative study aims to investigate consumers’ perceptions toward dairy cow welfare in traditional mountain farms. While consumers’ understanding of conventional dairy production and animal welfare has already been investigated, how consumers perceive animal welfare in traditional mountain dairy farming remains still unexplored. Focus group interviews were conducted with consumers having different degrees of geographical proximity to mountains and with an explicit interest in local dairy products. The results of this qualitative study show that participants expect mountain farming to be on a smaller scale when compared to non-mountain farming systems and expect mountain products to be healthier. Similarly, all participants consider origin, locality, and small-scale production as relevant quality attributes of mountain cheese. However, the appreciation of these abstract features did not necessarily result in their recognition when sample pictures of traditional husbandry systems were provided especially in the case of urban participants. This study contributes to reveal the gap between urban consumers’ conception of mountain farming and the actual farming practices. It also indicates the need to promote an effective science-based dialogue on animal welfare that goes beyond an anthropomorphic perspective and tackles the complexity of farming systems in relation to the context in which they are located.
Cancer-Associated Function of 2-Cys Peroxiredoxin Subtypes as a Survival Gatekeeper
Authors: Kang Sang Won , Lee Sunmi , Lee Joanna H. S.
Published: November 2018

Cancer cells are abnormal cells that do not comply with tissue homeostasis but undergo uncontrolled proliferation. Such abnormality is driven mostly by somatic mutations on oncogenes and tumor suppressors. Cancerous mutations show intra-tumoral heterogeneity across cancer types and eventually converge into the self-activation of proliferative signaling. While transient production of intracellular reactive oxygen species (ROS) is essential for cell signaling, its persistent production is cytotoxic. Thus, cancer cells require increased levels of intracellular ROS for continuous proliferation, but overexpress cellular peroxidase enzymes, such as 2-Cys peroxiredoxins, to maintain ROS homeostasis. However, suppression of 2-Cys peroxiredoxins has also been reported in some metastatic cancers. Hence, the cancer-associated functions of 2-Cys peroxiredoxins must be illuminated in the cellular context. In this review, we describe the distinctive signaling roles of 2-Cys peroxiredoxins beyond their intrinsic ROS-scavenging role in relation to cancer cell death and survival.
Exercise and Redox Status Responses Following Alpha-Lipoic Acid Supplementation in G6PD Deficient Individuals
Authors: Georgakouli Kalliopi , Fatouros Ioannis G. , Fragkos Apostolos , Tzatzakis Theofanis , Deli Chariklia K. , Papanikolaou Konstantinos , Koutedakis Yiannis , Jamurtas Athanasios Z.
Published: November 2018

G6PD deficiency renders cells more susceptible to oxidative insults, while antioxidant dietary supplementation could restore redox balance and ameliorate exercise-induced oxidative stress. To examine the effects of alpha-lipoic acid (ALA) supplementation on redox status indices in G6PD deficient individuals, eight male adults with G6PD deficiency (D) participated in this randomized double-blind placebo-controlled crossover trial. Participants were randomly assigned to receive ALA (600 mg/day) or placebo for 4 weeks separated by a 4-week washout period. Before and at the end of each treatment period, participants exercised following an exhaustive treadmill exercise protocol. Blood samples were obtained before (at rest), immediately after and 1h after exercise for later analysis of total antioxidant capacity (TAC), uric acid, bilirubin, thiobarbituric acid reactive substances (TBARS) and protein carbonyls (PC). ALA resulted in significantly increased resting TAC and bilirubin concentrations. Moreover, TAC increased immediately and 1h after exercise following both treatment periods, whereas bilirubin increased immediately after and 1h after exercise following only ALA. No significant change in uric acid, TBARS or PC was observed at any time point. ALA supplementation for 4 weeks may enhance antioxidant status in G6PD individuals; however, it does not affect redox responses to acute exercise until exhaustion or exercise performance.
Characterization of TrxC, an Atypical Thioredoxin Exclusively Present in Cyanobacteria
Authors: López-Maury Luis , Heredia-Martínez Luis G. , Florencio Francisco J.
Published: November 2018

Cyanobacteria form a diverse group of oxygenic photosynthetic prokaryotes considered to be the antecessor of plant chloroplast. They contain four different thioredoxins isoforms, three of them corresponding to m, x and y type present in plant chloroplast, while the fourth one (named TrxC) is exclusively found in cyanobacteria. TrxC has a modified active site (WCGLC) instead of the canonical (WCGPC) present in most thioredoxins. We have purified it and assayed its activity but surprisingly TrxC lacked all the classical activities, such as insulin precipitation or activation of the fructose-1,6-bisphosphatase. Mutants lacking trxC or over-expressing it were generated in the model cyanobacterium Synechocystis sp. PCC 6803 and their phenotypes have been analyzed. The ΔtrxC mutant grew at similar rates to WT in all conditions tested although it showed an increased carotenoid content especially under low carbon conditions. Overexpression strains showed reduced growth under the same conditions and accumulated lower amounts of carotenoids. They also showed lower oxygen evolution rates at high light but higher Fv’/Fm’ and Non-photochemical-quenching (NPQ) in dark adapted cells, suggesting a more oxidized plastoquinone pool. All these data suggest that TrxC might have a role in regulating photosynthetic adaptation to low carbon and/or high light conditions.
Introducing Bentonite into the Environment in the Construction Stage of Linear Underground Investment Using the HDD Method
Authors: Kwast-Kotlarek Urszula , Hełdak Maria , Szczepański Jakub
Published: November 2018

The study discusses the issue of introducing drilling fluid (bentonite) into the environment during the construction of linear underground investments, considering the example of the construction of the high-pressure gas pipeline, Czeszów—Kiełczów DN1000, which involved the use of the Horizontal Directional Drilling (HDD) method. The analyses concern the drilling stage as a low-waste technology, indicating the use and management of bentonite drilling fluid in a closed circulation cycle in the area of Pęciszów, poviat Trzebnica (Poland). The loss of drilling fluid in valuable natural areas during the construction stage of the gas pipeline has been analyzed. Drilling fluid is an element of the horizontal directional drilling technology (HDD). The analyzed area included a section of the route of the strategic gas pipeline, realized in June–July 2017 in an area of lowland ash and alder forest 91E0-3, a probable breeding site of the Bluethroat for a detailed description and common frog. The loss of the drilling fluid used in the drilling phase has been determined, depending on the type of soil and the related fraction as well as the possibility of treating the drilling fluid used to construct the drilling.
Moving towards Smart Cities: A Selection of Middleware for Fog-to-Cloud Services
Authors: Bangui Hind , Rakrak Said , Raghay Said , Buhnova Barbora
Published: November 2018

Smart cities aim at integrating various IoT (Internet of Things) technologies by providing many opportunities for the development, governance, and management of user services. One of the ways to support this idea is to use cloud and edge computing techniques to reduce costs, manage resource consumption, enhance performance, and connect the IoT devices more effectively. However, the selection of services remains a significant research question since there are currently different strategies towards cloud computing, including services for central remote computing (traditional cloud model) as well as distributed local computing (edge computing). In this paper, we offer an integrated view of these two directions and the selection among the edge technologies based on MCDA (Multiple Criteria Decision Analysis) algorithms. To this end, we propose a foglet as a middleware that aims at achieving satisfactory levels of customer services by using fuzzy similarity and TOPSIS (Technique for Order of Preference by Similarity to Ideal Solution) to facilitate the rating and selection of services in the fog-to-cloud environment. Then, we describe the selection process with a numerical example, and conclude our work with an outline of future perspectives.
Nonlinear Ultrasonic Detection Method for Delamination Damage of Lined Anti-Corrosion Pipes Using PZT Transducers
Authors: Hong Xiaobin , Liu Yuan , Lin Xiaohui , Luo Zongqiang , He Zhenwei
Published: November 2018

Lined anti-corrosion pipes are widely used in oil and gas, petrochemical, pharmaceutical industries. However, defects, especially delamination, may occur in the production and service of pipes which result in safety accidents. Based on nonlinear ultrasonic theory, this paper studied the delamination detection method using the nonlinear harmonics for lined anti-corrosion pipes. The response characteristics of the anti-corrosion pipe were obtained through a sweep sine response experiment and the preferred excitation frequency was determined. The Wavelet Packet transform and Hilbert–Huang transform is applied for signal process and feature extraction. Then, a series of experiments were carried out and the results were analyzed and discussed. The results showed that a second-order and third-order nonlinear coefficient increased with the delamination damage. The amplitude of second-harmonic is much stronger than the third-order one. The mean squared error of the nonlinear coefficient, which was processed by Wavelet Packet transform and Hilbert–Huang transform, is smaller than wavelet packet transform and Discrete Fourier transform or processed only Hilbert–Huang transform. The higher harmonics can describe the change of delamination damage, which means that the nonlinear ultrasonic detection method could use for damage detection of anti-corrosion pipe. The nonlinear higher-harmonic is sensitive to delamination damage. The nonlinear ultrasonic method has the potential for damage detection for lined anti-corrosion pipes.
A New Approach to the Determination of Mineral and Organic Soil Types Based on Dilatometer Tests (DMT)
Authors: Rabarijoely Simon
Published: November 2018

In order to identify the soil type in the ground, Marchetti’s nomogram chart is commonly used on the basis of dilatometer tests (DMT). In this chart, the material index values (ID) and the dilatometer modulus (ED) are used to determine the state and type of soils predominant in mineral soils. Unfortunately, this classification is not accurate enough for the identification of organic soils. This article proposes a new classification based on a nomogram chart for both mineral soils and organic soils using (p0), (p1) readings and pore water pressure (uo).
Source Contributions to Ozone Formation in the New South Wales Greater Metropolitan Region, Australia
Authors: Nguyen Duc Hiep , Chang Lisa T.-C. , Trieu Toan , Salter David , Scorgie Yvonne
Published: November 2018

Ozone and fine particles (PM2.5) are the two main air pollutants of concern in the New South Wales Greater Metropolitan Region (NSW GMR) due to their contribution to poor air quality days in the region. This paper focuses on source contributions to ambient ozone concentrations for different parts of the NSW GMR, based on source emissions across the greater Sydney region. The observation-based Integrated Empirical Rate model (IER) was applied to delineate the different regions within the GMR based on the photochemical smog profile of each region. Ozone source contribution was then modelled using the CCAM-CTM (Cubic Conformal Atmospheric model-Chemical Transport model) modelling system and the latest air emission inventory for the greater Sydney region. Source contributions to ozone varied between regions, and also varied depending on the air quality metric applied (e.g., average or maximum ozone). Biogenic volatile organic compound (VOC) emissions were found to contribute significantly to median and maximum ozone concentration in North West Sydney during summer. After commercial and domestic sources, power generation was found to be the next largest anthropogenic source of maximum ozone concentrations in North West Sydney. However, in South West Sydney, beside commercial and domestic sources, on-road vehicles were predicted to be the most significant contributor to maximum ozone levels, followed by biogenic sources and power stations. The results provide information that policy makers can use to devise various options to control ozone levels in different parts of the NSW Greater Metropolitan Region.
Performance of Adaptive Unstructured Mesh Modelling in Idealized Advection Cases over Steep Terrains
Authors: Li Jinxi , Zheng Jie , Zhu Jiang , Fang Fangxin , Pain Christopher. C. , Steppeler Jürgen , Navon Ionel M. , Xiao Hang
Published: November 2018

Advection errors are common in basic terrain-following (TF) coordinates. Numerous methods, including the hybrid TF coordinate and smoothing vertical layers, have been proposed to reduce the advection errors. Advection errors are affected by the directions of velocity fields and the complexity of the terrain. In this study, an unstructured adaptive mesh together with the discontinuous Galerkin finite element method is employed to reduce advection errors over steep terrains. To test the capability of adaptive meshes, five two-dimensional (2D) idealized tests are conducted. Then, the results of adaptive meshes are compared with those of cut-cell and TF meshes. The results show that using adaptive meshes reduces the advection errors by one to two orders of magnitude compared to the cut-cell and TF meshes regardless of variations in velocity directions or terrain complexity. Furthermore, adaptive meshes can reduce the advection errors when the tracer moves tangentially along the terrain surface and allows the terrain to be represented without incurring in severe dispersion. Finally, the computational cost is analyzed. To achieve a given tagging criterion level, the adaptive mesh requires fewer nodes, smaller minimum mesh sizes, less runtime and lower proportion between the node numbers used for resolving the tracer and each wavelength than cut-cell and TF meshes, thus reducing the computational costs.
Considering Rain Gauge Uncertainty Using Kriging for Uncertain Data
Authors: Cecinati Francesca , Moreno-Ródenas Antonio M. , Rico-Ramirez Miguel A. , Ten Veldhuis Marie-claire , Langeveld Jeroen G.
Published: November 2018

In urban hydrological models, rainfall is the main input and one of the main sources of uncertainty. To reach sufficient spatial coverage and resolution, the integration of several rainfall data sources, including rain gauges and weather radars, is often necessary. The uncertainty associated with rain gauge measurements is dependent on rainfall intensity and on the characteristics of the devices. Common spatial interpolation methods do not account for rain gauge uncertainty variability. Kriging for Uncertain Data (KUD) allows the handling of the uncertainty of each rain gauge independently, modelling space- and time-variant errors. The applications of KUD to rain gauge interpolation and radar-gauge rainfall merging are studied and compared. First, the methodology is studied with synthetic experiments, to evaluate its performance varying rain gauge density, accuracy and rainfall field characteristics. Subsequently, the method is applied to a case study in the Dommel catchment, the Netherlands, where high-quality automatic gauges are complemented by lower-quality tipping-bucket gauges and radar composites. The case study and the synthetic experiments show that considering measurement uncertainty in rain gauge interpolation usually improves rainfall estimations, given a sufficient rain gauge density. Considering measurement uncertainty in radar-gauge merging consistently improved the estimates in the tested cases, thanks to the additional spatial information of radar rainfall data but should still be used cautiously for convective events and low-density rain gauge networks.
Furanoid F-Acid F6 Uniquely Induces NETosis Compared to C16 and C18 Fatty Acids in Human Neutrophils
Authors: Khan Meraj , Pace-Asciak Cecil , Al-Hassan Jassim , Afzal Mohammad , Liu Yuan , Oommen Sosamma , Paul Bincy , Nair Divya , Palaniyar Nades
Published: November 2018

Various biomolecules induce neutrophil extracellular trap (NET) formation or NETosis. However, the effect of fatty acids on NETosis has not been clearly established. In this study, we focused on the NETosis-inducing ability of several lipid molecules. We extracted the lipid molecules present in Arabian Gulf catfish (Arius bilineatus, Val) skin gel, which has multiple therapeutic activities. Gas chromatography–mass spectrometry (GC-MS) analysis of the lipid fraction-3 from the gel with NETosis-inducing activity contained fatty acids including a furanoid F-acid (F6; 12,15-epoxy-13,14-dimethyleicosa-12,14-dienoic acid) and common long-chain fatty acids such as palmitic acid (PA; C16:0), palmitoleic acid (PO; C16:1), stearic acid (SA; C18:0), and oleic acid (OA; C18:1). Using pure molecules, we show that all of these fatty acids induce NETosis to different degrees in a dose-dependent fashion. Notably, F6 induces a unique form of NETosis that is rapid and induces reactive oxygen species (ROS) production by both NADPH oxidase (NOX) and mitochondria. F6 also induces citrullination of histone. By contrast, the common fatty acids (PA, PO, SA, and OA) only induce NOX-dependent NETosis. The activation of the kinases such as ERK (extracellular signal-regulated kinase) and JNK (c-Jun N-terminal kinase) is important for long-chain fatty acid-induced NETosis, whereas, in F-acid-induced NETosis, Akt is additionally needed. Nevertheless, NETosis induced by all of these compounds requires the final chromatin decondensation step of transcriptional firing. These findings are useful for understanding F-acid- and other fatty acid-induced NETosis and to establish the active ingredients with therapeutic potential for regulating diseases involving NET formation.
Induction of Recombinant Lectin Expression by an Artificially Constructed Tandem Repeat Structure: A Case Study Using Bryopsis plumosa Mannose-Binding Lectin
Authors: Hwang Hyun-Ju , Han Jin-Woo , Jeon Hancheol , Han Jong Won
Published: November 2018

Lectin is an important protein in medical and pharmacological applications. Impurities in lectin derived from natural sources and the generation of inactive proteins by recombinant technology are major obstacles for the use of lectins. Expressing recombinant lectin with a tandem repeat structure can potentially overcome these problems, but few studies have systematically examined this possibility. This was investigated in the present study using three distinct forms of recombinant mannose-binding lectin from Bryopsis plumosa (BPL2)—i.e., the monomer (rD1BPL2), as well as the dimer (rD2BPL2), and tetramer (rD4BPL2) arranged as tandem repeats. The concentration of the inducer molecule isopropyl β-D-1-thiogalactopyranoside and the induction time had no effect on the efficiency of the expression of each construct. Of the tested constructs, only rD4BPL2 showed hemagglutination activity towards horse erythrocytes; the activity of towards the former was 64 times higher than that of native BPL2. Recombinant and native BPL2 showed differences in carbohydrate specificity; the activity of rD4BPL2 was inhibited by the glycoprotein fetuin, whereas that of native BPL2 was also inhibited by d-mannose. Our results indicate that expression as tandem repeat sequences can increase the efficiency of lectin production on a large scale using a bacterial expression system.
Alcoholic Liver Disease: Alcohol Metabolism, Cascade of Molecular Mechanisms, Cellular Targets, and Clinical Aspects
Authors: Teschke Rolf
Published: November 2018

Alcoholic liver disease is the result of cascade events, which clinically first lead to alcoholic fatty liver, and then mostly via alcoholic steatohepatitis or alcoholic hepatitis potentially to cirrhosis and hepatocellular carcinoma. Pathogenetic events are linked to the metabolism of ethanol and acetaldehyde as its first oxidation product generated via hepatic alcohol dehydrogenase (ADH) and the microsomal ethanol-oxidizing system (MEOS), which depends on cytochrome P450 2E1 (CYP 2E1), and is inducible by chronic alcohol use. MEOS induction accelerates the metabolism of ethanol to acetaldehyde that facilitates organ injury including the liver, and it produces via CYP 2E1 many reactive oxygen species (ROS) such as ethoxy radical, hydroxyethyl radical, acetyl radical, singlet radical, superoxide radical, hydrogen peroxide, hydroxyl radical, alkoxyl radical, and peroxyl radical. These attack hepatocytes, Kupffer cells, stellate cells, and liver sinusoidal endothelial cells, and their signaling mediators such as interleukins, interferons, and growth factors, help to initiate liver injury including fibrosis and cirrhosis in susceptible individuals with specific risk factors. Through CYP 2E1-dependent ROS, more evidence is emerging that alcohol generates lipid peroxides and modifies the intestinal microbiome, thereby stimulating actions of endotoxins produced by intestinal bacteria; lipid peroxides and endotoxins are potential causes that are involved in alcoholic liver injury. Alcohol modifies SIRT1 (Sirtuin-1; derived from Silent mating type Information Regulation) and SIRT2, and most importantly, the innate and adapted immune systems, which may explain the individual differences of injury susceptibility. Metabolic pathways are also influenced by circadian rhythms, specific conditions known from living organisms including plants. Open for discussion is a 5-hit working hypothesis, attempting to define key elements involved in injury progression. In essence, although abundant biochemical mechanisms are proposed for the initiation and perpetuation of liver injury, patients with an alcohol problem benefit from permanent alcohol abstinence alone.
BCAbox Algorithm Expands Capabilities of Raman Microscope for Single Organelles Assessment
Authors: Kuzmin Andrey N. , Pliss Artem , Rzhevskii Alex , Lita Adrian , Larion Mioara
Published: November 2018

Raman microspectroscopy is a rapidly developing technique, which has an unparalleled potential for in situ proteomics, lipidomics, and metabolomics, due to its remarkable capability to analyze the molecular composition of live cells and single cellular organelles. However, the scope of Raman spectroscopy for bio-applications is limited by a lack of software tools for express-analysis of biomolecular composition based on Raman spectra. In this study, we have developed the first software toolbox for immediate analysis of intracellular Raman spectra using a powerful biomolecular component analysis (BCA) algorithm. Our software could be easily integrated with commercial Raman spectroscopy instrumentation, and serve for precise analysis of molecular content in major cellular organelles, including nucleoli, endoplasmic reticulum, Golgi apparatus, and mitochondria of either live or fixed cells. The proposed software may be applied in broad directions of cell science, and serve for further advancement and standardization of Raman spectroscopy.
The Multiple Hit Hypothesis for Gulf War Illness: Self-Reported Chemical/Biological Weapons Exposure and Mild Traumatic Brain Injury
Authors: Janulewicz Patricia , Krengel Maxine , Quinn Emily , Heeren Timothy , Toomey Rosemary , Killiany Ronald , Zundel Clara , Ajama Joy , O’Callaghan James , Steele Lea , Klimas Nancy , Sullivan Kimberly
Published: November 2018

The Gulf War Illness Consortium (GWIC) was designed to identify objective biomarkers of Gulf War Illness (GWI) in 1991 Gulf War veterans. The symptoms of GWI include fatigue, pain, cognitive problems, gastrointestinal, respiratory, and skin problems. Neurotoxicant exposures during deployment, such as pesticides, sarin, and pyridostigmine bromide pills have been identified as contributors to GWI. We have also found an association between mild traumatic brain injury (mTBI) and increased rates of GWI. However, the combined impact of these physical and chemical exposures has not yet been explored in GWI. The objective of this study was to examine both self-reported mTBI and exposure to chemical/biological weapons (CBW) as a multiple or two hit model for increased risk of GWI and other chronic health conditions. The study population included 125 Gulf War (GW) veterans from the Boston GWIC. Exposure to CBW was reported in 47.2% of the study population, and 35.2% reported sustaining a mTBI during the war. Results confirmed that those with both exposures (mTBI and CBW) had higher rates of comorbid chronic health conditions while rates of GWI were equivalent for mTBI and CBW or mTBI alone. The timing of exposure to mTBI was found to be strikingly different between those with GWI and those without it. Correspondingly, 42.3% of GWI cases reported experiencing a mTBI during military service while none of the controls did (p = 0.0002). Rates of mTBI before and after the war did not differ between the cases and controls. In addition, 54% of cases compared to 14.3% of controls (p = <0.001) reported being exposed to CBW during military service. The current study examined the relation of the separate and combined effects of exposure to mTBI and CBW in 1991 GW veterans. The findings from this study suggest that both exposure to mTBI and CBW are associated with the development of GWI and multiple chronic health conditions and that combined exposure appears to lead to higher risk of chronic health effects.
Isolated Mammillary Bodies Damage—An Atypical Presentation of Wernicke Syndrome
Authors: Abbas Samar A. , Abboud Halim , Chalah Moussa A. , Sabbagh Chadi , Ayache Samar S.
Published: November 2018

We report atypical magnetic resonance imaging (MRI) lesions in a case of Wernicke encephalopathy. The patient presented with isolated anterograde amnesia following a partial colectomy complicated by peritonitis. Fluid-attenuated inversion recovery and T2 MRI sequences were normal. However, bilateral contrast enhancement of mammillary bodies was shown on T1 gadolinium-enhanced sequences. Blood tests revealed thiamine deficiency. The diagnosis of Wernicke encephalopathy was made and thiamine supplementation was given, resulting in complete recovery of the memory functions.
Cell Origins of High-Grade Serous Ovarian Cancer
Authors: Kim Jaeyeon , Park Eun , Kim Olga , Schilder Jeanne , Coffey Donna , Cho Chi-Heum , Bast Robert
Published: November 2018

High-grade serous ovarian cancer, also known as high-grade serous carcinoma (HGSC), is the most common and deadliest type of ovarian cancer. HGSC appears to arise from the ovary, fallopian tube, or peritoneum. As most HGSC cases present with widespread peritoneal metastases, it is often not clear where HGSC truly originates. Traditionally, the ovarian surface epithelium (OSE) was long believed to be the origin of HGSC. Since the late 1990s, the fallopian tube epithelium has emerged as a potential primary origin of HGSC. Particularly, serous tubal intraepithelial carcinoma (STIC), a noninvasive tumor lesion formed preferentially in the distal fallopian tube epithelium, was proposed as a precursor for HGSC. It was hypothesized that STIC lesions would progress, over time, to malignant and metastatic HGSC, arising from the fallopian tube or after implanting on the ovary or peritoneum. Many clinical studies and several mouse models support the fallopian tube STIC origin of HGSC. Current evidence indicates that STIC may serve as a precursor for HGSC in high-risk women carrying germline BRCA1 or 2 mutations. Yet not all STIC lesions appear to progress to clinical HGSCs, nor would all HGSCs arise from STIC lesions, even in high-risk women. Moreover, the clinical importance of STIC remains less clear in women in the general population, in which 85–90% of all HGSCs arise. Recently, increasing attention has been brought to the possibility that many potential precursor or premalignant lesions, though composed of microscopically—and genetically—cancerous cells, do not advance to malignant tumors or lethal malignancies. Hence, rigorous causal evidence would be crucial to establish that STIC is a bona fide premalignant lesion for metastatic HGSC. While not all STICs may transform into malignant tumors, these lesions are clearly associated with increased risk for HGSC. Identification of the molecular characteristics of STICs that predict their malignant potential and clinical behavior would bolster the clinical importance of STIC. Also, as STIC lesions alone cannot account for all HGSCs, other potential cellular origins of HGSC need to be investigated. The fallopian tube stroma in mice, for instance, has been shown to be capable of giving rise to metastatic HGSC, which faithfully recapitulates the clinical behavior and molecular aspect of human HGSC. Elucidating the precise cell(s) of origin of HGSC will be critical for improving the early detection and prevention of ovarian cancer, ultimately reducing ovarian cancer mortality.
Next-Generation Service Delivery: A Scoping Review of Patient Outcomes Associated with Alternative Models of Genetic Counseling and Genetic Testing for Hereditary Cancer
Authors: McCuaig Jeanna M. , Armel Susan Randall , Care Melanie , Volenik Alexandra , Kim Raymond H. , Metcalfe Kelly A.
Published: November 2018

The combination of increased referral for genetic testing and the current shortage of genetic counselors has necessitated the development and implementation of alternative models of genetic counseling and testing for hereditary cancer assessment. The purpose of this scoping review is to provide an overview of the patient outcomes that are associated with alternative models of genetic testing and genetic counseling for hereditary cancer, including germline-only and tumor testing models. Seven databases were searched, selecting studies that were: (1) full-text articles published ≥2007 or conference abstracts published ≥2015, and (2) assessing patient outcomes of an alternative model of genetic counseling or testing. A total of 79 publications were included for review and synthesis. Data-charting was completed using a data-charting form that was developed by the study team for this review. Seven alternative models were identified, including four models that involved a genetic counselor: telephone, telegenic, group, and embedded genetic counseling models; and three models that did not: mainstreaming, direct, and tumor-first genetic testing models. Overall, these models may be an acceptable alternative to traditional models on knowledge, patient satisfaction, psychosocial measures, and the uptake of genetic testing; however, particular populations may be better served by traditional in-person genetic counseling. As precision medicine initiatives continue to advance, institutions should consider the implementation of new models of genetic service delivery, utilizing a model that will best serve the needs of their unique patient populations.
Focal Adhesion Genes Refine the Intermediate-Risk Cytogenetic Classification of Acute Myeloid Leukemia
Authors: Pallarès Victor , Hoyos Montserrat , Chillón M. Carmen , Barragán Eva , Prieto Conde M. Isabel , Llop Marta , Falgàs Aïda , Céspedes María Virtudes , Montesinos Pau , Nomdedeu Josep F. , Brunet Salut , Sanz Miguel Ángel , González-Díaz Marcos , Sierra Jorge , Mangues Ramon , Casanova Isolda
Published: November 2018

In recent years, several attempts have been made to identify novel prognostic markers in patients with intermediate-risk acute myeloid leukemia (IR-AML), to implement risk-adapted strategies. The non-receptor tyrosine kinases are proteins involved in regulation of cell growth, adhesion, migration and apoptosis. They associate with metastatic dissemination in solid tumors and poor prognosis. However, their role in haematological malignancies has been scarcely studied. We hypothesized that PTK2/FAK, PTK2B/PYK2, LYN or SRC could be new prognostic markers in IR-AML. We assessed PTK2, PTK2B, LYN and SRC gene expression in a cohort of 324 patients, adults up to the age of 70, classified in the IR-AML cytogenetic group. Univariate and multivariate analyses showed that PTK2B, LYN and PTK2 gene expression are independent prognostic factors in IR-AML patients. PTK2B and LYN identify a patient subgroup with good prognosis within the cohort with non-favorable FLT3/NPM1 combined mutations. In contrast, PTK2 identifies a patient subgroup with poor prognosis within the worst prognosis cohort who display non-favorable FLT3/NPM1 combined mutations and underexpression of PTK2B or LYN. The combined use of these markers can refine the highly heterogeneous intermediate-risk subgroup of AML patients, and allow the development of risk-adapted post-remission chemotherapy protocols to improve their response to treatment.
Epigenetic Regulation by lncRNAs: An Overview Focused on UCA1 in Colorectal Cancer
Authors: Neve Bernadette , Jonckheere Nicolas , Vincent Audrey , Van Seuningen Isabelle
Published: November 2018

Colorectal cancers have become the second leading cause of cancer-related deaths. In particular, acquired chemoresistance and metastatic lesions occurring in colorectal cancer are a major challenge for chemotherapy treatment. Accumulating evidence shows that long non-coding (lncRNAs) are involved in the initiation, progression, and metastasis of cancer. We here discuss the epigenetic mechanisms through which lncRNAs regulate gene expression in cancer cells. In the second part of this review, we focus on the role of lncRNA Urothelial Cancer Associated 1 (UCA1) to integrate research in different types of cancer in order to decipher its putative function and mechanism of regulation in colorectal cancer cells. UCA1 is highly expressed in cancer cells and mediates transcriptional regulation on an epigenetic level through the interaction with chromatin modifiers, by direct regulation via chromatin looping and/or by sponging the action of a diversity of miRNAs. Furthermore, we discuss the role of UCA1 in the regulation of cell cycle progression and its relation to chemoresistance in colorectal cancer cells.
Catalytic Ethylene Oligomerization over Ni/Al-HMS: A Key Step in Conversion of Bio-Ethanol to Higher Olefins
Authors: Liu Yanyong
Published: November 2018

Al-modified hexagonal mesoporous silica (HMS) materials were synthesized using dodecylamine as a template according to the methods reported in the literature. FT-IR spectra proved that Al3+ ions entered in the HMS framework in Al-HMS (prepared by sol-gel reaction) but Al3+ ions existed in the extra-framework in Al/HMS (prepared by post-modification). NH3-TPD indicated that either Al-HMS or Al/HMS had solid acid sites on the surface, and the acidic strength of Al/HMS was stronger than that of Al-HMS. For ethylene oligomerization at 200 °C under 1 MPa, Ni/Al-HMS showed an ethylene conversion of 96.3%, which was much higher than that over Ni/Al/HMS (45.6%). The selectivity for C4H8, C6H12, C8H16, and C8+ was 37.7%, 24.5%, 24.0%, and 9.1% for ethylene oligomerization over Ni/Al-HMS, respectively. Ni/Al-MCM-41, which has been reported as an effective catalyst for ethylene oligomerization in the literature, showed a high ethylene conversion (95.2%) similar to that of Ni/Al-HMS in this study. However, the selectivity for C8H16 over Ni/Al-MCM-41 (16.3%) was lower than that over Ni/Al-HMS (24.0%) in the ethylene oligomerization. For ethanol dehydration at 300 °C under 1 MPa, a commercial H-ZSM-5 catalyst showed a high ethylene yield (91.2%) after reaction for 24 h using a feed containing 90 wt.% ethanol and 10 wt.% water. In this study, a two-step process containing two fixed-bed reactors and one cold trap was designed to achieve the direct synthesis of higher olefins from bio-ethanol. The cold trap was used to collect the water formed from ethanol dehydration. By using H-ZSM-5 as a catalyst for ethanol dehydration in the first reactor and using Ni/Al-HMS as a catalyst for ethylene oligomerization in the second reactor, higher olefins were continuously formed by feeding a mixture containing 90 wt.% ethanol and 10 wt.% water. The yields of higher olefins did not decrease after reaction for 8 h in the two-step reaction system.
Low Pt Alloyed Nanostructures for Fuel Cells Catalysts
Authors: Huang Shuoyuan , Shan Aixian , Wang Rongming
Published: November 2018

Low-noble metal electrocatalysts are attracting massive attention for anode and cathode reactions in fuel cells. Pt transition metal alloy nanostructures have demonstrated their advantages in high performance low-noble metal electrocatalysts due to synergy effects. The basic of designing this type of catalysts lies in understanding structure-performance correlation at the atom and electron level. Herein, design threads of highly active and durable Pt transition metal alloy nanocatalysts are summarized, with highlighting their synthetic realization. Microscopic and electron structure characterization methods and their prospects will be introduced. Recent progress will be discussed in high active and durable Pt transition metal alloy nanocatalysts towards oxygen reduction and methanol oxidation, with their structure-performance correlations illustrated. Lastly, an outlook will be given on promises and challenges in future developing of Pt transition metal alloy nanostructures towards fuel cells catalysis uses.
Elucidation and Characterization of New Chlorinated By-Products after Electrochemical Degradation of Hydrochlorothiazide Using Graphite–Poly Vinyl Chloride Electrode
Authors: Mussa Zainab Haider , Al-Qaim Fouad Fadhil , Yuzir Ali , Hara Hirofumi , Azman Shamila , Chelliapan Shreeshivadasan
Published: November 2018

This paper describes an electrochemical treatment process of hydrochlorothiazide (HDZ) under different conditions such as initial concentration, sodium chloride and applied voltage. In this present study, HDZ was treated by electrochemical oxidation process using graphite-PVC composite electrode as anode and Platinum (Pt) as cathode. All results were analyzed using liquid chromatography-time of flight/mass spectrometry (LC-TOF/MS). It was found that at high applied voltages, and high amounts of NaCl, the electrochemical treatment process was more efficient. The removal% of HDZ was 92% at 5 V after 60 min. From the obtained results, the electrochemical oxidation process of HDZ followed pseudo first order with rate constant values ranged between 0.0009 and 0.0502 min−1, depending on the experimental conditions. Energy consumption was also considered in this study, it was ranged between 0.9058 and 5.56 Wh/mg using 0.5, 0.3 and 0.1 g NaCl within interval times of (10, 20, 30, 40, 50, 60, 70, and 80 min). Five chlorinated and one non-chlorinated by-products were formed and analyzed in negative ionization (NI) mode during the electrochemical process. Due to the strong oxidizing potential of the chlorine (Cl2) and hypochlorite ion (ClO−), HDZ and its by-products were removed after 140 min. Furthermore, a novel synthesis of chlorothiaizde as one of the new by-products was reported in this present study. Toxicity was impacted by the formation of the by-products, especially at 20 min. The inhibition percentage (I%) of E. coli bacteria was decreased to be the lowest value after 140 min.
Label-Free Biosensors Based onto Monolithically Integrated onto Silicon Optical Transducers
Authors: Angelopoulou Michailia , Kakabakos Sotirios , Petrou Panagiota
Published: November 2018

The article reviews the current status of label-free integrated optical biosensors focusing on the evolution over the years of their analytical performance. At first, a short introduction to the evanescent wave optics is provided followed by detailed description of the main categories of label-free optical biosensors, including sensors based on surface plasmon resonance (SPR), grating couplers, photonic crystals, ring resonators, and interferometric transducers. For each type of biosensor, the detection principle is first provided followed by description of the different transducer configurations so far developed and their performance as biosensors. Finally, a short discussion about the current limitations and future perspectives of integrated label-free optical biosensors is provided.
A Web-Based Calculator for the Prediction of Severe Neurodevelopmental Impairment in Preterm Infants Using Clinical and Imaging Characteristics
Authors: Vesoulis Zachary A. , El Ters Nathalie M. , Herco Maja , Whitehead Halana V. , Mathur Amit M.
Published: November 2018

Although the most common forms of brain injury in preterm infants have been associated with adverse neurodevelopmental outcomes, existing MRI scoring systems lack specificity, do not incorporate clinical factors, and are technically challenging to perform. The objective of this study was to develop a web-based, clinically-focused prediction system which differentiates severe neurodevelopmental outcomes from normal-moderate outcomes at two years. Infants were retrospectively identified as those who were born ≤30 weeks gestation and who had MRI imaging at term-equivalent age and neurodevelopmental testing at 18–24 months. Each MRI was scored on injury in three domains (intraventricular hemorrhage, white matter injury, and cerebellar hemorrhage) and clinical factors that were strongly predictive of an outcome were investigated. A binary logistic regression model was then generated from the composite of clinical and imaging components. A total of 154 infants were included (mean gestational age = 26.1 ± 1.8 weeks, birth weight = 889.1 ± 226.2 g). The final model (imaging score + ventilator days + delivery mode + antenatal steroids + retinopathy of prematurity requiring surgery) had strong discriminatory power for severe disability (AUC = 0.850), with a PPV (positive predictive value) of 76% and an NPV (negative predictive value) of 90%. Available as a web-based tool, it can be useful for prognostication and targeting early intervention services to infants who may benefit the most from such services.
Preparation of Ti–Zr-Based Conversion Coating on 5052 Aluminum Alloy, and Its Corrosion Resistance and Antifouling Performance
Authors: Zhang Hehong , Zhang Xiaofeng , Zhao Xuhui , Tang Yuming , Zuo Yu
Published: November 2018

A chemical conversion coating on 5052 aluminum alloy was prepared by using K2ZrF6 and K2TiF6 as the main salts, KMnO4 as the oxidant and NaF as the accelerant. The surface morphology, structure and composition were analyzed by SEM, EDS, FT–IR and XPS. The corrosion resistance of the conversion coating was studied by salt water immersion and polarization curve analysis. The influence of fluorosilane (FAS-17) surface modification on its antifouling property was also discussed. The results showed that the prepared conversion coating mainly consisted of AlF3·3H2O, Al2O3, MnO2 and TiO2, and exhibited good corrosion resistance. Its corrosion potential in 3.5 wt % NaCl solution was positively shifted about 590 mV and the corrosion current density was dropped from 1.10 to 0.48 μA cm−2. By sealing treatment in NiF2 solution, its corrosion resistance was further improved yielding a corrosion current density drop of 0.04 μA cm−2. By fluorosilane (FAS-17) surface modification, the conversion coating became hydrophobic due to low-surface-energy groups such as CF2 and CF3, and the contact angle reached 136.8°. Moreover, by FAS-17 modification, the corrosion resistance was enhanced significantly and its corrosion rate decreased by about 25 times.
A Low-Temperature Alumina/Copper Diffusion Bonding Process using La-Doped Titanium Interlayers
Authors: Su Cherng-Yuh , Huang Jia-Liang , Chen Po-Chun , Yu Hsin-Jung , Ma Dai-Liang , Yu Bang-Ying
Published: November 2018

Ceramic-to-metal heterojunctions have been established to improve high-temperature stability for applications in aerospace and harsh environments. In this work, we employed low-temperature diffusion bonding to realize an alumina/Cu heterogeneous joint. Using a thin layer of lanthanum-doped titanium (La-doped Ti) to metallize the alumina surface, we achieved the bonding at a temperature range of 250–350 °C. We produced a uniform, thermally stable, and high-strength alumina/Cu joint after a hot-press process in vacuum. Signals from X-ray diffraction (XRD) suggested the successful diffusion of Ti and La into the alumina substrate, as Ti can easily substitute Al in alumina, and La has a better oxygen affinity than that of Al. The transmission electron microscopy and XRD results also showed the existence of CuxTiyO phases without CuxTiy or LaOx. In addition, the bonding strength of alumina/copper hot-pressed at 250, 300, and 350 °C were 7.5, 9.8 and 15.0 MPa, respectively. The process developed in this study successfully lowered the bonding temperature for the alumina/copper joint.
Sputtering Physical Vapour Deposition (PVD) Coatings: A Critical Review on Process Improvement and Market Trend Demands
Authors: Baptista Andresa , Silva Francisco , Porteiro Jacobo , Míguez José , Pinto Gustavo
Published: November 2018

Physical vapour deposition (PVD) is a well-known technology that is widely used for the deposition of thin films regarding many demands, namely tribological behaviour improvement, optical enhancement, visual/esthetic upgrading, and many other fields, with a wide range of applications already being perfectly established. Machining tools are, probably, one of the most common applications of this deposition technique, sometimes used together with chemical vapour deposition (CVD) in order to increase their lifespan, decreasing friction, and improving thermal properties. However, the CVD process is carried out at higher temperatures, inducing higher stresses in the coatings and substrate, being used essentially only when the required coating needs to be deposited using this process. In order to improve this technique, several studies have been carried out optimizing the PVD technique by increasing plasma ionization, decreasing dark areas (zones where there is no deposition into the reactor), improving targets use, enhancing atomic bombardment efficiency, or even increasing the deposition rate and optimizing the selection of gases. These studies reveal a huge potential in changing parameters to improve thin film quality, increasing as well the adhesion to the substrate. However, the process of improving energy efficiency regarding the industrial context has not been studied as deeply as required. This study aims to proceed to a review regarding the improvements already studied in order to optimize the sputtering PVD process, trying to relate these improvements with the industrial requirements as a function of product development and market demand.
Profiling Director’s Style Based on Camera Positioning Using Fuzzy Logic
Authors: Junaedi Hartarto , Hariadi Mochamad , Purnama I Ketut Eddy
Published: November 2018

Machinima is a computer imaging technology typically used in games and animation. It prints all movie cast properties into a virtual environment by means of a camera positioning. Since cinematography is complementary to Machinima, it is possible to simulate a director’s style via various camera placements in this environment. In a gaming application, the director’s style is one of the most impressive cinematic factors, where a whole different gaming experience can be obtained using different styles applied to the same scene. This paper describes a system capable of automatically profile a director’s style using fuzzy logic. We employed 19 output variables and 15 other calculated variables from the animation extraction data to profile two different directors’ styles from five scenes. Area plots and histograms were generated, and, by analyzing the histograms, different director’s styles could be subsequently classified.