bluuebunny/crossref_metadata_2025 huggingface dataset:
An immunogenic cell death-related classification predicts prognosis and response to immunotherapy in kidney renal clear cell carcinoma
Authors: Wang Licheng , Zhu Yaru , Ren Zhen , Sun Wenhuizi , Wang Zhijing , Zi Tong , Li Haopeng , Zhao Yan , Qin Xin , Gao Dacheng , Zhang Libo , He Ziyang , Le Wei , Wu Qiang , Wu Gang
Published: August 2023

Introduction

Immunogenic cell death (ICD) is a form of regulated cell death that activates an adaptive immune response in an immunocompetent host and is particularly sensitive to antigens from tumor cells. Kidney clear cell carcinoma (KIRC) is an immunogenic tumor with extensive tumor heterogeneity. However, no reliable predictive biomarkers have been identified to reflect the immune microenvironment and therapeutic response of KIRC.

Methods

Therefore, we used the CIBERSORT and ESTIMATE algorithms to define three ICD clusters based on the expression of ICD-related genes in 661 KIRC patients. Subsequently, we identified three different ICD gene clusters based on the overlap of differentially expressed genes (DEGs) within the ICD clusters. In addition, principal component analysis (PCA) was performed to calculate the ICD scores.

Results

The results showed that patients with reduced ICD scores had a poorer prognosis and reduced transcript levels of immune checkpoint genes regulated with T cell differentiation. Furthermore, the ICD score was negatively correlated with the tumor mutation burden (TMB) value of KICD. patients with higher ICD scores showed clinical benefits and advantages of immunotherapy, indicating that the ICD score is an accurate and valid predictor to assess the effect of immunotherapy.

Discussion

Overall, our study presents a comprehensive KICD immune-related ICD landscape that can provide guidance for current immunotherapy and predict patient prognosis to help physicians make judgments about the patient’s disease and treatment modalities, and can guide current research on immunotherapy strategies for KICD.
Bilateral adrenal giant medullary lipoma combined with disorders of sex development: a rare case report and literature review
Authors: Zhanghuang Chenghao , Long Na , Yang Zhen , Xie Yucheng
Published: August 2023

Bilateral adrenal myelolipoma is rare in clinics and patients with disorders of sex development (DSDs). One case was reported in our center. A 45-year-old patient was admitted to the hospital after discovering a left abdominal mass for more than a year and worsening abdominal pain for 18 days. An imaging examination showed bilateral adrenal masses. Physical examination showed clitoris hypertrophy with patelliform changes, thick and dense pubic hair, normal development of bilateral labia majora without labia minora, and urethral opening. After the relevant preoperative examinations, bilateral adrenal mass resection was performed under general anesthesia. The postoperative pathology confirmed adrenal myelolipoma. The incision healed well without recurrence over 10 years after the operation. Her enlarged clitoris decreased in size. This case report has a detailed diagnosis and treatment process and sufficient examination results. It can provide a reference for diagnosing and treating patients with bilateral adrenal myelolipoma and DSD and reduce the risk of misdiagnosis and mistreatment.
Cancer data quality and harmonization in Europe: the experience of the BENCHISTA Project – international benchmarking of childhood cancer survival by stage
Authors: Lopez-Cortes Angela , Didonè Fabio , Botta Laura , Hjalgrim Lisa L. , Jakab Zsuzsanna , Cañete Nieto Adela , Stiller Charles , Zeller Bernward , Gatta Gemma , Pritchard-Jones Kathy ,   The BENCHISTA Project Working Group
Published: August 2023

Introduction

Variation in stage at diagnosis of childhood cancers (CC) may explain differences in survival rates observed across geographical regions. The BENCHISTA project aims to understand these differences and to encourage the application of the Toronto Staging Guidelines (TG) by Population-Based Cancer Registries (PBCRs) to the most common solid paediatric cancers.

Methods

PBCRs within and outside Europe were invited to participate and identify all cases of Neuroblastoma, Wilms Tumour, Medulloblastoma, Ewing Sarcoma, Rhabdomyosarcoma and Osteosarcoma diagnosed in a consecutive three-year period (2014-2017) and apply TG at diagnosis. Other non-stage prognostic factors, treatment, progression/recurrence, and cause of death information were collected as optional variables. A minimum of three-year follow-up was required. To standardise TG application by PBCRs, on-line workshops led by six tumour-specific clinical experts were held. To understand the role of data availability and quality, a survey focused on data collection/sharing processes and a quality assurance exercise were generated. To support data harmonization and query resolution a dedicated email and a question-and-answers bank were created.

Results

67 PBCRs from 28 countries participated and provided a maximally de-personalized, patient-level dataset. For 26 PBCRs, data format and ethical approval obtained by the two sponsoring institutions (UCL and INT) was sufficient for data sharing. 41 participating PBCRs required a Data Transfer Agreement (DTA) to comply with data protection regulations. Due to heterogeneity found in legal aspects, 18 months were spent on finalizing the DTA. The data collection survey was answered by 68 respondents from 63 PBCRs; 44% of them confirmed the ability to re-consult a clinician in cases where stage ascertainment was difficult/uncertain. Of the total participating PBCRs, 75% completed the staging quality assurance exercise, with a median correct answer proportion of 92% [range: 70% (rhabdomyosarcoma) to 100% (Wilms tumour)].

Conclusion

Differences in interpretation and processes required to harmonize general data protection regulations across countries were encountered causing delays in data transfer. Despite challenges, the BENCHISTA Project has established a large collaboration between PBCRs and clinicians to collect detailed and standardised TG at a population-level enhancing the understanding of the reasons for variation in overall survival rates for CC, stimulate research and improve national/regional child health plans.
Combined α2- and D2-receptor blockade activates noradrenergic and dopaminergic neurons, but extracellular dopamine in the prefrontal cortex is determined by uptake and release from noradrenergic terminals
Authors: Sagheddu Claudia , Devoto Paola , Aroni Sonia , Saba Pierluigi , Pistis Marco , Gessa Gian Luigi
Published: August 2023

Experimental and clinical evidence indicates a deficit of release and function of dopamine in schizophrenia and suggests that α2-adrenoceptor antagonists rescue dopamine deficit and improve the antipsychotic efficacy of D2-receptor antagonists. In anesthetized male rats, we investigated how the blockade of α2- and D2-receptors by atipamezole and raclopride, respectively, modified the firing of noradrenergic neurons in the locus coeruleus (LC) and dopaminergic neurons in the ventral tegmental area (VTA). In freely moving rats, we studied how atipamezole and raclopride modified extracellular noradrenaline, dopamine, and DOPAC levels in the medial prefrontal cortex (mPFC) through microdialysis. When administered alone, atipamezole activated LC noradrenaline but not VTA dopamine cell firing. Combined with raclopride, atipamezole activated dopamine cell firing above the level produced by raclopride. Atipamezole increased extracellular dopamine to the same level, whether administered alone or combined with raclopride. In the presence of the noradrenaline transporter (NET) inhibitor, atipamezole combined with raclopride increased extracellular dopamine beyond the level produced by either compound administered alone. The results suggest that a) the D2-autoreceptor blockade is required for LC noradrenaline to activate VTA cell firing; b) the level of dopamine released from dopaminergic terminals is determined by NET; c) the elevation of extracellular dopamine levels in the mPFC is the resultant of dopamine uptake and release from noradrenergic terminals, independent of dopaminergic cell firing and release; and d) LC noradrenergic neurons are an important target for treatments to improve the prefrontal deficit of dopamine in neuropsychiatric pathologies.
Visual reflexive attention as a useful measure of development
Authors: Lundwall Rebecca A.
Published: August 2023

Cognitive psychology began over three-quarters of a century ago and we have learned a great deal in that time, including concerning the development of cognitive abilities such as perception, attention, and memory, all of which develop across infancy and childhood. Attention is one aspect of cognition that is vital to success in a variety of life activities and, arguably, the foundation of memory, learning, problem solving, decision making, and other cognitive activities. The cognitive abilities of later childhood and adulthood generally appear to depend on the reflexes, abilities, and skills of infancy. Research in developmental cognitive science can help us understand adult cognition and know when to intervene when cognitive function is at risk. This area of research can be challenging because, even in typical development, the course of cognitive development for a particular child does not always improve monotonically. In addition, the typical trajectory of this development has been understood differently from different historical perspectives. Neither the history of thought that has led to our current understanding of attention (including its various types) nor the importance of developmental aspects of attention are frequently covered in training early career researchers, especially those whose primary area of research in not attention. My goal is to provide a review that will be useful especially to those new to research in the subfield of attention. Sustained attention in adults and children has been well-studied, but a review of the history of thought on the development of reflexive attention with a focus on infancy is overdue. Therefore, I draw primarily on historical and modern literature and clarify confusing terminology as it has been used over time. I conclude with examples of how cognitive development research can contribute to scientific and applied progress.
Factors influencing nurses’ post-traumatic growth during the COVID-19 pandemic: Bayesian network analysis
Authors: Yao Xi , Wang Junyi , Yang Yingrui , Zhang Hongmei
Published: August 2023

Objective

During the COVID-19 pandemic, nurses, especially if females and working in intensive care units or emergencies unit, were much more at risk than other health-workers categories to develop malaise and acute stress symptoms. This study aimed to examine the nurses’ post-traumatic growth and associated influencing factors during the COVID-19 pandemic.

Methods

A cross-sectional study using an online survey was conducted at Henan Provincial People’s Hospital to gather data from nurses. A set of questionnaires was used to measure the participants’ professional identity, organizational support, psychological resilience and post-traumatic growth. Univariate, correlation, and multiple linear regression analyses were used to determine significant factors influencing post-traumatic growth. A theoretical framework based on the Bayesian network was constructed to understand post-traumatic growth and its associated factors comprehensively.

Results

In total, 1,512 nurses participated in the study, and a moderate-to-high level of post-traumatic growth was reported. After screening, the identified variables, including psychological counseling, average daily working hours, average daily sleep duration, professional identity, organizational support, and psychological resilience, were selected to build a Bayesian network model. The results of Bayesian network showed that professional identity and psychological resilience positively affected post-traumatic growth directly, which was particularly pronounced in low- and high-scoring groups. While organizational support positively affected post-traumatic growth indirectly.

Conclusion

Although this study identified a moderate-to-high level of nurses’ post-traumatic growth, proactive measures to improve psychological resilience fostered by professional identity and organizational support should be prioritized by hospitals and nursing managers.
The relationship between psychological resilience and depression among the diabetes patients under the background of “dynamic zero COVID-19”: the mediating role of stigma and the moderating role of medication burden
Authors: Mei Yujin , Yang Xue , Gui JiaoFeng , Li YuQing , Zhang XiaoYun , Wang Ying , Chen Wenyue , Chen Mingjia , Liu Changjun , Zhang Lin
Published: August 2023

Objective

Depression in diabetes patients is caused by their own disease or the surrounding social environment. How to cope with changes in mentality and adjust psychological stress responses, especially under China’s dynamic zero COVID-19 policy, is worth further discussion. The researchers constructed a moderated mediation model to test the effect of psychological resilience during dynamic zero COVID-19 on depression in diabetes patients and the mediating role of stigma and the moderating effect of medication burden.

Method

From June to September, 2022, data were collected in Jinghu District, Wuhu City, Anhui Province, by multi-stage stratified sampling. Firstly, we selected a tertiary hospital randomly in Jinghu District. Secondly, departments are randomly chosen from the hospital. Finally, we set up survey points in each department and randomly select diabetes patients. In addition, we used the Connor-Davidson Elasticity Scale (CD-RISC) to measure psychological resilience of patients, and used the Stigma Scale for Chronic Illness (SSCI) to measure stigma, medication burden was measured by the Diabetes Treatment Burden Scale (DTBQ), and depression was assessed by the Patient Health Questionnaire-9 (PHQ-9). We used SPSS (version 23.0) and PROCESS (version 4.1) for data analysis.

Results

(1) Psychological resilience was negatively correlated with stigma, medication burden, and depression. Stigma was positively associated with medication burden and depression. Medication burden and depression are positively correlated, (2) The mediation analysis showed that psychological resilience had a direct predictive effect on depression, and stigma partially mediated the relationship, and (3) Medication burden moderates the direct pathway by which psychological resilience predicts depression; Medication burden moderates the first half of “psychological resilience → stigma → depression.”

Conclusion

Under the mediating effect of stigma, psychological resilience can improve depression. Medication burden has a moderating effect on the relationship between psychological resilience and depression, and it also has a moderating effect on the relationship between psychological resilience and stigma. These results facilitate the understanding of the relationship mechanisms between psychological resilience and depression.
The environment and dry eye—manifestations, mechanisms, and more
Authors: Patel Sneh , Mittal Rhiya , Kumar Naresh , Galor Anat
Published: August 2023

Dry eye disease (DED) is a multifactorial condition that often presents with chronic symptoms of pain (that can be characterized as “dryness,” “burning,” and “irritation,” to name a few) and/or fluctuating or poor-quality vision. Given its multifactorial nature, several pathophysiologic mechanisms have been identified that can underlie symptoms, including tear film, ocular surface, and/or corneal somatosensory nerve abnormalities. Research has focused on understanding how environmental exposures can increase the risk for DED flares and negatively impact the tear film, the ocular surface, and/or nerve health. Given that DED is a common condition that negatively impacts physical and mental functioning, managing DED requires multiple strategies. These can include both medical approaches and modulating adverse environmental conditions, the latter of which may be a cost-effective way to avoid DED flares. Thus, an understanding of how environmental exposures relate to disease is important. This Review summarizes research on the relationships between environmental exposures and DED, in the hope that this information will engage healthcare professionals and patients to consider environmental manipulations in their management of DED.
Effects of Nitrogen Fertilization and Plant Density on Proso Millet (Panicum miliaceum L.) Growth and Yield under Mediterranean Pedoclimatic Conditions
Authors: Palchetti Enrico , Moretta Michele , Calamai Alessandro , Mancini Marco , Dell’Acqua Matteo , Brilli Lorenzo , Armanasco Paolo , Masoni Alberto
Published: August 2023

In recent years, the dry-land cereal proso millet has become an interesting crop for cultivation in the Mediterranean environment due to the consequences of climate change. It can be considered a resilient crop because it is particularly successful in extreme drought and high-temperature conditions. The goals of this research study were to compare different plant densities (D) and nitrogen fertilization rates (N) in millet (Panicum miliaceum), evaluating morphological, productive, and phenological traits. A 2-year field experiment was carried out in Italy, and millet (var. Sunrise) was subjected to four nitrogen fertilization rates (0, 50, 100, and 150 kg N ha−1) in interaction with three plant densities (55, 111, and 222 plants m2). Significant differences were found in all the investigated plant traits. The highest grain yield data (i.e., 3.211 kg ha−1 and 3.263 kg ha−1) and total biomass (i.e., 11.464 kg ha−1 and 11.760 kg ha−1) were obtained with the N rate of 150 kg ha−1 and density of 222 plants m2. Regarding protein content, the highest values were observed using N50, N100, and N150 (ranging from 10.03% to 10.14%) and with D55 (10.43%). Phenological parameters were affected by both plant density and nitrogen amount and decreased when higher levels of these two factors were employed.
The Potential of Adjuvants Used with Microbiological Control of Insect Pests with Emphasis on Organic Farming
Authors: Holka Małgorzata , Kowalska Jolanta
Published: August 2023

Biological plant protection is a crucial component of integrated pest management strategies. It is considered a safer alternative to chemical plant protection, with reduced risks to human health and the environment. The significance of biological plant protection has been on the rise, driven by the European Union’s mandate to decrease the reliance on chemical pesticides, the discontinuation of certain chemical active substances, and their limited availability. Microbiological plant protection products find application in organic farming systems. Among these, mycoinsecticides are prominent examples, utilizing insecticidal fungi such as Beauveria bassiana, Cordyceps fumosoroseus, C. farinosa, and Metarhizium anisopliae complex. Due to the high sensitivity of these organisms to unfavorable weather and environmental conditions, their use in the protection of field crops may not bring the desired effect. The enhancement of their efficacy may be accomplished through the use of adjuvants. Adjuvants are substances incorporated into plant protection products, including microbial insecticides, or used alone to enhance their effectiveness. They can play a pivotal role in improving the performance of mycoinsecticides by ensuring better coverage on plant surfaces and increasing the likelihood of successful pest control, thereby contributing to the overall success of biological methods of pest control. Consequently, it becomes imperative to investigate the impact of various adjuvants on the survival and effectiveness of microorganisms. Furthermore, there is no officially approved list of adjuvants for use in organic farming, the use of inadequate adjuvant may result in failure to obtain an organic certificate. The origin of adjuvants determines their classification, which significantly impacts for employment in organic farming practices. Included tables provide a list of adjuvants and additives known to enhance the efficacy of pest and disease control.
Genetic and Phenotypic Characterization of Subclinical Mastitis-Causing Multidrug-Resistant Staphylococcus aureus
Authors: Silva Amanda Thaís Ferreira , Gonçalves Juliano Leonel , Dantas Stéfani Thais Alves , Rall Vera Lúcia Mores , de Oliveira Pollyanne Raysa Fernandes , dos Santos Marcos Veiga , Peixoto Rodolfo de Moraes , Mota Rinaldo Aparecido
Published: August 2023

The core objective of this study was to genetically and phenotypically characterize subclinical mastitis-causing multidrug-resistant Staphylococcus aureus (MDRSA). In addition, risk factors associated with subclinical mastitis caused by MDRSA were investigated. Bacterial cultures were performed on 2120 mammary quarters, 40 swabs of milk utensils, 5 bulk tank milk samples, and 11 nostril and 11 hand swabs from milkers from five dairy farms. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was conducted for S. aureus identification. Antimicrobial resistance was screened phenotypically using the disk diffusion test in all S. aureus isolates. A biofilm formation assay; detection of genes associated with beta-lactam resistance, efflux pump, and biofilm formation; and pulsed-field gel electrophoresis (PFGE) were performed in all MDRSA isolates. Multi-locus sequence typing (MLST) was carried out in cefoxitin-resistant MDRSA isolates. A total of 188 S. aureus isolates from milk as well as two from milking utensils and one from bulk tank milk were identified. Most of the isolates (92.7%; 177 of 191) showed beta-lactam resistance, and 7% (14 of 191) were MDRSA. Interestingly, 36% (5 of 14) of MDRSA isolates were cefoxitin-resistant, but none carried mecA or mecC genes. Based on PFGE results, it was observed that S. aureus strains were more likely to be unique to a specific herd. Two clonal complexes were identified, CC97 (ST126; commonly livestock-associated) and CC1 (ST7440; usually community-associated). To the best of our knowledge, this is the first report of ST7440 isolated from bovine mastitis in Brazil. The risk factor results underscored the importance of considering parity, stage of lactation, SCC, milk production, and herd size when studying the risk of subclinical mastitis and antimicrobial resistance in S. aureus. Thus, to implement effective strategies to prevent subclinical mastitis in dairy herds and to minimize MDRSA spread, it is important to understand MDRSA strains’ distribution and their antimicrobial resistance profile.
Enzyme Production and Inhibitory Potential of Pseudomonas aeruginosa: Contrasting Clinical and Environmental Isolates
Authors: Aqel Hazem , Sannan Naif , Foudah Ramy , Al-Hunaiti Afnan
Published: August 2023

(1) Background: This study summarizes the findings of two studies investigating the inhibitory effects of Pseudomonas aeruginosa strains from clinical and environmental sources against gram-positive and gram-negative bacteria and fungi. The studies also analyzed the correlation between enzyme production and inhibitory effects to gain insights into the antimicrobial capabilities of P. aeruginosa strains; (2) Methods: Both studies employed similar methodologies, including the use of disk diffusion and well diffusion methods to assess the inhibitory effects of P. aeruginosa strains against target pathogens. Enzyme production was analyzed through various biochemical assays to determine the diversity and frequencies of enzyme secretion among the strains; (3) Results: A comparative analysis of enzyme production in P. aeruginosa strains from clinical sources revealed significant variations in enzyme production, with hemolysin and protease being the most commonly produced enzymes. Gelatinase production showed lower rates, whereas chondroitinase and hyaluronidase were absent or occurred less frequently. In contrast, a comparative analysis of enzyme production in environmental isolates showed different patterns, indicating adaptation to environmental conditions. Pyocyanin production was absent in all environmental isolates. The inhibitory effects against gram-positive and gram-negative bacteria varied among different P. aeruginosa strains, with strain-specific variations observed. Limited inhibitory effects were observed against fungi, primarily toward gram-positive bacteria; (4) Conclusions: The findings highlight the strain-specific nature of inhibitory effects and enzyme production in P. aeruginosa strains. The correlation between enzyme production and inhibitory effects against gram-positive bacteria suggest a potential role of specific enzymes, such as hemolysin and protease, in the antimicrobial activity. The complexity of the relationship between enzyme production and the inhibition of different pathogens requires further investigation. The results emphasize the potential of P. aeruginosa strains as sources for antimicrobial strategies, particularly against gram-positive bacteria. Future research should focus on understanding the mechanisms underlying these inhibitory effects and exploring their therapeutic applications.
Effects of Dietary Supplementation of Lacticaseibacillus Chiayiensis AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao Chicks
Authors: Kang Xin , Li Xin-Dong , Luo Cheng-Ying , Xin Wei-Gang , Zhou Huan-Yu , Wang Feng , Lin Lian-Bing
Published: August 2023

The growing issue of antibiotic resistance has restrained the utilization of antibiotics as growth enhancers in the poultry industry. Probiotics are candidates for replacing antibiotics in the poultry industry. However, probiotics are strain-specific and their efficacy needs to be investigated before applying them. The aim of this study was to assess the positive effects of Lacticaseibacillus chiayiensis AACE3 on the health and gut microbiota of Nandan Yao chicks. The results showed that compared with the blank control (NC) and aureomycin (PC) groups, L. chiayiensis AACE3 increased final body weight (BW), villus height and improved the ratio of villus height to crypt depth in chicken jejunal tissues. L. chiayiensis AACE3 also increased the activity of hepatic antioxidant enzymes (SOD, CAT and T-AOC) and reduced hepatic oxidative damage (MDA). Furthermore, compared to NC, L. chiayiensis AACE3, the activity of intestinal digestive enzymes (i.e., α-amylase, lipase and trypsin) was increased. L. chiayiensis AACE3 upregulated the production of IgA and IgG and downregulated the production of IL-6, IL-1β and TNF-α in chicken serum. Moreover, supplementation of L. chiayiensis AACE3 enhances the diversity of gut microbes. At the phylum level, the abundance of Actinobacteriota and Proteobacteria decreased with L. chiayiensis AACE3 supplementation, while the abundance of Verrucomicrobiota and Bacteroidetes increased. At the genus level, there was an increase in the abundance of potential probiotics Akkermansia, Romboutsia, Subdoligranulum, and Lactobacillus. This study confirms that L. chiayiensis AACE3 is an excellent feed additive as an alternative to aureomycin and offers various advantages for the healthy growth of chickens during the brooding period by positively affecting their gut microbiome.
Genome-Wide Analysis of the Polyphenol Oxidase Gene Family in Olea europaea Provides Insights into the Mechanism of Enzymatic Browning in Olive Fruit
Authors: Liu Qingqing , Wang Chenhe , Cui Qizhen , Fan Yutong , Zhang Jianguo , Rao Guodong
Published: August 2023

Browning of olive (Olea europaea L.) fruit reduces the sensory and nutritional qualities of olive oil, thereby increasing production costs. Polyphenol oxidases (PPOs) are the key enzymes that catalyze phenolic substance oxidation and mediate enzymatic browning in olive fruit, but the exact regulatory mechanism remains unclear. The main challenge is the lack of comprehensive information on OePPOs at the genome-wide level. In this study, 18 OePPO genes were identified. Subsequently, we performed a bioinformatic analysis on them. We also analyzed the expression patterns and determined the relationship among browning degree, PPO activity, and expression of OePPOs in the fruits of three olive varieties. Based on our analysis, we identified the four most conserved motifs. OePPOs were classified into two groups, with OePPOs from Group 1 showing only diphenolase activity and OePPOs from Group 2 exhibiting both mono-/diphenolase activities. Seven pairs of gene duplication events were identified, and purifying selection was found to have played a critical role in the evolution of the OePPO gene family. A positive correlation was observed between the browning degree of olive fruit and PPO activity across different olive varieties. Moreover, two important genes were found: OePPO-5 the main effector gene responsible for fruit browning, and OePPO-8, a key gene associated with specialized metabolite synthesis in the olive fruit. In short, our discoveries provide a basis for additional functional studies on OePPO genes and can help elucidate the mechanism of enzymatic browning in olive fruit in the future.
To Prevent Oxidative Stress, What about Protoporphyrin IX, Biliverdin, and Bilirubin?
Authors: Martínez Ana , López-Rull Isabel , Fargallo Juan A.
Published: August 2023

The pigments responsible for eggshell color and patterning in birds are protoporphyrin IX (PP) and biliverdin (BV). Both are involved in the catalytic degradation of the hemo group. Bilirubin (BR), another pigment, is produced when BV is broken down. PP, BV, and BR are free radical scavengers. In this study, we theoretically investigated the antioxidant capacities of these three biological meaningful molecules using Density Functional Theory calculations. First, two antioxidant mechanisms were analyzed for PP, BV, and BR: electron transfer and Hydrogen Atom Transfer. Second, since PP and BV interact with the calcium carbonate matrix of the eggshell, we analyzed the interaction of these pigments with Ca2+ and investigated their chelate compounds. Third, we explored the pro-oxidant properties of PP and BV, which have been proposed for PP when photoactivated to the triplet state, but not for BV. Our results show that PP, BV, and BR are just as good antiradical as other important natural pigments (carotenoids). Neither the antiradical properties of PP and BV nor the UV-visible spectra change due to the presence of calcium, suggesting that the signaling function of these pigments is not affected by the link with Ca2+. Finally, we found that both PP and BV (alone and when linked to Ca2+) can transfer energy from its triplet state to molecular-oxygen-producing singlet oxygen, indicating their pro-oxidant capacity. This investigation answers important questions about the function of these pigments, which may help to understand their influence on the reproductive success of birds.
Inertial Propulsion of a Mobile Platform Driven by Two Eccentric Bodies
Authors: Timofte Stelica , Miclosina Calin-Octavian , Cojocaru Vasile , Gerocs Attila , Korka Zoltan-Iosif
Published: August 2023

In recent decades, enthusiasts and researchers from all over the world have invested a lot of energy and time in trying to develop linear propulsion systems using the inertial force generated by two masses revolving in opposite directions. The authors present an original concept for the propulsion system of a mobile platform by using the inertial force generated by two eccentric bodies that are revolving in opposite directions. Based on this approach, a wheeled vehicle was designed, built using additive manufacturing techniques, and finally tested. Validation of the concept and verification of the analytically derived kinematic parameters were carried out by 3D modeling and motion simulation performed in SolidWorks 2023. It was concluded that the initial position of the eccentric bodies determines the direction of movement. Moreover, a favorable correlation of the eccentric bodies’ masses with the entire platform mass is able to ensure the movement at an oscillating but permanently positive speed.
Decentralized Robust Control of a Network of Inverter-Based Distributed Generation Systems
Authors: Shojaee Milad , Azizi S. Mohsen
Published: August 2023

This paper presents the design of decentralized robust controllers for a network of inverter-based distributed generation systems with LC filters in the scale of a nanogrid. Using overlapping decomposition, the network of inverters is clustered into several subnetworks such that all inverters within a subnetwork are strongly coupled and there is no or a weak coupling effect between any two inverters from different subnetworks. For the inverters within the same subnetwork, decentralized robust controllers are designed sequentially in the μ-synthesis framework. In addition, all controllers are designed to be robust against ±10% variations in the LC filter parameters. To assess the performance of the proposed sequentially-designed controllers and compare it to that of the benchmark independently-designed ones, the distances between two neighboring inverters from the same and different subnetworks are considered to be 200 (m) and 800 (m), respectively. In this case, time-response and robustness analysis results illustrate the superiority of the proposed sequentially-designed controllers in the overlapping decomposition framework over the benchmark independently-designed ones. Moreover, transient overload and nonlinear load analyses demonstrate that the proposed sequentially-designed decentralized controllers are able to keep the load voltage within ±10% of the nominal value and the harmonic voltage distortions to less than 4%.
Research on the Influence of Subway Tunnel Depth on Heat Storage Characteristics of the Surrounding Soil Mass
Authors: Song Huaitao , Li Jingfen , Yu Yueyang , Chen Qianlong
Published: August 2023

With the long-term running of the subway, the soil layer around the tunnel takes on the thermal deposition effect, which can lead the air in the tunnel to heat up and pose a serious threat to the safety operation of trains. Through taking some subway tunnels from typical zones as an example, the influence of tunnel depth on the heat storage characteristics of the surrounding soil mass was analyzed in the paper. The results indicate that the temperature field of the surrounding soil mass was thermally disturbed by both the ground air temperature and the tunnel air temperature, and there was a significant coupling point ‘O’ located at the center of the tunnel overburden. With the extension of the heat-exchange time, the shape of the cooling ring around the tunnel gradually changed from a circle to an oval. For the analysis of cases, from the space aspect, when the tunnel depth was less than 30 m, the wall temperature increased gradually with the increase of tunnel depth. From the time aspect, over time, the wall temperature gradually rose and finally reached a fixed value. From the region aspect, the heat absorption capacity of different areas decreased gradually with the increase of tunnel depth. When the depth exceeded 45 m, the heat absorption capacity of certain cities became negative. In addition, three typical boundaries were discussed, and the optimal method for evaluating the heat absorption capacity of the tunnel soil was ultimately determined. This study has important reference value for temperature control and positioning problems in the process of tunnel construction and operation.
CBCT-Based Assessment of Vapor Lock Effects on Endodontic Disinfection
Authors: Puleio Francesco , Lizio Angelo Sergio , Coppini Valentina , Lo Giudice Roberto , Lo Giudice Giuseppe
Published: August 2023

Background: The phenomenon of vapor lock, characterized by the formation of gas bubbles, poses challenges in achieving effective cleaning and debridement during endodontic treatments. This study aimed to evaluate the presence of vapor lock and to quantify its volume in the root canal system. Methods: Ten single-rooted teeth were selected, and their root canals were shaped using the Protaper Next system and irrigated with 5% NaOCl and 17% EDTA. Then, the canals were irrigated with a solution made of contrast medium (8 M cesium chloride solution) mixed in equal proportions with 5% sodium hypochlorite. CBCT scans were performed to analyze the presence and volume of bubbles in different canal thirds. Statistical analysis was conducted to compare the number of bubbles and the percentage of space occupied by them. Results: The results demonstrated the presence of vapor lock in all treated teeth, with a higher concentration of gas bubbles in the apical canal third. The formation of gas bubbles hindered the cleaning and debridement procedures, particularly in the apical region. Conclusions: This study provides evidence of the occurrence of vapor lock and highlights the importance of enhancing the cleaning phase in endodontic treatments to minimize bubble formation or eliminate them once formed. These findings contribute to a better understanding of the challenges posed by vapor lock and suggest avenues for optimizing endodontic procedures.
Selective Change in the Bacteria Cultured and Isolated in Respiratory Sputum from Elderly Patients during the SARS-CoV-2 Pandemic
Authors: Nagasawa Masayuki , Kato Tomoyuki , Tanaka Ippei , Ono Emi
Published: August 2023

The SARS-CoV-2 pandemic has affected social patterns and consequently the prevalence of infections, such as seasonal influenza. It has been reported that invasive pneumococcal infection has markedly decreased worldwide. Method: We retrospectively investigated the bacteria cultured and isolated from 23,052 respiratory sputum samples obtained at our hospital from April 2015 to March 2022. The average patient age was 71.8 years old, with a standard deviation of 16.0 years old. There was no significant difference in the age of the patients or the female-to-male ratio between each year. The detection ratio of bacteria was analyzed in accordance with sputum quality based on the Geckler classification. Results: The detection ratio of community-acquired pneumonia pathogens such as Haemophilus influenzae, Moraxella catarrhalis, and Streptococcus pneumoniae increased in parallel with the quality of the sputum, while that of hospital-acquired pneumonia pathogens such as Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus was not significantly affected by the quality of the sputum. The detection ratio of former pathogens in the good-quality respiratory sputum had decreased significantly since April 2020 by 60–80%, while that of P. aeruginosa and S. aureus had increased by 40–50%. Conclusions: The SARS-CoV-2 pandemic reduced the detection ratio of H. influenzae, M. catarrhalis, and S. pneumoniae but increased that of P. aeruginosa and S. aureus in the good-quality respiratory sputum from elderly patients. The influence of this selective change in isolated bacteria on the health and comorbidity of elderly patients remains to be investigated.
Space Weather Effects on Satellites
Authors: Miteva Rositsa , Samwel Susan W. , Tkatchova Stela
Published: August 2023

The study presents a concise overview on the main effects on satellites due to space weather drivers compared to the well-known interplanetary, magnetospheric and ground-based consequences. The solar-activity-driven influences include specific physics-based effects on the spacecraft surface and on-board electronics due to electromagnetic emission and energetic particles as well as complex effects due to geomagnetic storms which may endanger the mission performance and spacecraft longevity. We select as test examples the Starlink satellites in the period 2019–2022 and present the temporal correspondence between their launches and the space weather phenomena. Based on comparative analysis, we discuss whether the occurrence vs. the intensity of solar and interplanetary drivers of space weather can be considered as a cause for orbital stability problems and satellite loss. The results suggest that a sequence of geomagnetic disturbances together with multiple weak space weather events could lead to severe levels of atmospheric drag ending in a service or satellite loss.
Quantum Astronomy at the University and INAF Astronomical Observatory of Padova, Italy
Authors: Barbieri Cesare , Naletto Giampiero , Zampieri Luca
Published: August 2023

Twenty years ago, we started to apply quantum optics to the astronomical research carried out inside the Department of Physics and Astronomy and the INAF Astronomical Observatory in Padova, Italy. The initial activities were stimulated by the project of the European Southern Observatory (ESO) to build a 100 m diameter telescope, the Overwhelmingly Large (OWL) telescope. The enormous photon flux expected from such an aperture suggested that quantum optics concepts be utilized in order to obtain novel astrophysical results. Following initial successful attempts to utilize the orbital angular momentum of the light beam to enhance the visibility of faint companions to bright stars, the Padova team concentrated its efforts on very high time resolution, in order to measure and store the arrival time of celestial photons to better than one nanosecond. To obtain observational results, we built two photon counting photometers (AquEye and IquEye) to be used with our telescopes of the Asiago Observatory and with 4 m class telescopes such as the ESO New Technology Telescope (NTT) in Chile. This paper firstly describes these two instruments and then expounds the results obtained on pulsar light curves, lunar occultations and the first photon counting intensity interferometry measurements of the bright star Vega. Indeed, the correlation of photon arrival times on two or more apertures can lead to extremely high angular resolutions, as shown around 1970 by Hanbury Brown and Twiss. Prospects for quantum intensity interferometry with arrays of Cherenkov light telescopes will also be described.
Crosslinked Hyperbranched Polyglycerol-Based Polymer Electrolytes for Lithium Metal Batteries
Authors: Neumann Niklas , Abels Gideon , Koschek Katharina , Boskamp Laura
Published: August 2023

Tailored partially methylated and methacrylated hyperbranched polyglycerols (hbPG-MAx/OMey) combined with lithium bis(trifluoromethanesulfonyl)imide (LiTFSI) as conducting salt were investigated after crosslinking with respect to their application as solid polymer electrolytes (SPE) in lithium metal batteries. For sample preparation and coating, a straightforward solvent-free photopolymerization method was applied. With the aim of finding the right balance between mechanical and electrochemical properties, electrolytes with different crosslinking densities were studied. High crosslink density increases mechanical integrity but reduces local chain motion and thus ionic conductivity at the same time. Differential scanning calorimetry (DSC), chronoamperometric and impedance measurements show that the hyperbranched polyether structure interacts strongly with lithium cations. Finally, the SPE with the lowest crosslinking density was selected and investigated in cycling tests due to the parameters of highest absolute values in conductivity (2.1 × 10−6 S cm−1 at 30 °C; 2.0 × 10−5 S cm−1 at 60 °C), lowest Tg (from DSC: −39 °C), electrochemical stability window (4.3 V vs. Li/Li+) and mechanical strength (1.6 ± 0.4 MPa at 25 °C). At low C-rates and elevated temperatures (60 °C), cells were cycled with high Coulombic efficiency. At high C-rates, a distinct decrease in specific capacity was observed due to insufficient ionic conductivity.
A Systemically Administered Unconjugated Antisense Oligonucleotide Targeting DUX4 Improves Muscular Injury and Motor Function in FSHD Model Mice
Authors: Kakimoto Tetsuhiro , Ogasawara Akira , Ishikawa Kiyoshi , Kurita Takashi , Yoshida Kumiko , Harada Shuichi , Nonaka Taeko , Inoue Yoshimi , Uchida Keiko , Tateoka Takashi , Ohta Tetsuya , Kumagai Shinji , Sasaki Takashi , Aihara Hajime
Published: August 2023

Facioscapulohumeral muscular dystrophy (FSHD), one of the most common muscular dystrophies, is caused by an abnormal expression of the DUX4 gene in skeletal muscles, resulting in muscle weakness. In this study, we investigated MT-DUX4-ASO, a novel gapmer antisense oligonucleotide (ASO). MT-DUX4-ASO decreased the expression of DUX4 and its target genes in FSHD patient-derived myoblasts. For the first time, we demonstrated that a systemically administered ASO, even without a ligand for drug delivery, could significantly improve muscle injury and motor function in the ACTA1-MCM/FLExDUX4 (DUX4-TG) mouse model of FSHD. Tamoxifen (TMX) injection transiently induces skeletal-muscle-specific DUX4 expression in DUX4-TG mice, while the skeletal muscles of TMX-untreated DUX4-TG mice have leaky DUX4 expression in a small subset of myofibers similar to those of FSHD patients. Subcutaneous 10 mg/kg of MT-DUX4-ASO at two-week intervals significantly suppressed muscular DUX4 target gene expression, histological muscle injury, and blood muscle injury marker elevation in TMX-untreated DUX4-TG mice. Notably, MT-DUX4-ASO at 10 mg/kg every other week significantly prevented the TMX-induced declines in treadmill test running speed and muscle force in DUX4-TG mice. Thus, the systemically administered unconjugated MT-DUX4-ASO suppressed disease progression in DUX4-TG mice, extending the potential of unconjugated ASOs as a promising FSHD treatment strategy.
Clot Retraction and Its Correlation with the Function of Platelet Integrin αIIbβ3
Authors: Gao Daniel , Sun Caroline W. , Woodley Angela B. , Dong Jing-fei
Published: August 2023

Clot retraction results from retractions of platelet filopodia and fibrin fibers and requires the functional platelet αIIbβ3 integrin. This assay is widely used to test the functions of platelets and fibrinogen as well as the efficacy of fibrinolysis. Changes in clot retraction have been found in a variety of hemostatic abnormalities and, more recently, in arterial thrombosis. Despite its broad clinical use and low cost, many aspects of clot retraction are poorly understood. In the present study, we performed two clinical standard clot retraction assays using whole-blood and platelet-rich plasma (PRP) samples to determine how clot retraction correlates with platelet counts and mean volume, the density of αIIbβ3 integrin and PLA genotypes, and plasma fibrinogen levels. We found that clot retraction was affected by platelet counts, but not mean platelet volume. It correlated with the surface density of the integrin αIibβ3, but not PLA genotypes. These results indicate that clot retraction measures a unique aspect of platelet function and can serve as an additional means to detect functional changes in platelets.
Can Graphene Pave the Way to Successful Periodontal and Dental Prosthetic Treatments? A Narrative Review
Authors: Apostu Alina Mihaela , Sufaru Irina-Georgeta , Tanculescu Oana , Stoleriu Simona , Doloca Adrian , Ciocan Pendefunda Alice Arina , Solomon Sorina Mihaela
Published: August 2023

Graphene, as a promising material, holds the potential to significantly enhance the field of dental practices. Incorporating graphene into dental materials imparts enhanced strength and durability, while graphene-based nanocomposites offer the prospect of innovative solutions such as antimicrobial dental implants or scaffolds. Ongoing research into graphene-based dental adhesives and composites also suggests their capacity to improve the quality and reliability of dental restorations. This narrative review aims to provide an up-to-date overview of the application of graphene derivatives in the dental domain, with a particular focus on their application in prosthodontics and periodontics. It is important to acknowledge that further research and development are imperative to fully explore the potential of graphene and ensure its safe use in dental practices.