bluuebunny/crossref_metadata_2025 huggingface dataset:
A Rapid and Reliable Absorbance Assay to Identify Drug–Drug Interactions with Thiopurine Drugs
Authors: Russell Drake A. , Stafford Carson , Totah Rheem A.
Published: December 2024

Background: Thiopurine methyltransferase (TPMT) plays a crucial role in the detoxification of thiopurine drugs, including the antimetabolites azathioprine and 6-mercaptopurine (6-MP) used to treat autoimmune diseases and various cancers. These drugs interfere with DNA synthesis by inhibiting the production of purine-containing nucleotides, leading to the death of rapidly dividing cells. TPMT inactivates thiopurine drugs by methylating at the thiol group. The activity of TPMT can vary significantly between individuals, and its activity is impacted by co-administered drugs, altering the effectiveness and toxicity of thiopurine drugs. TPMT is inhibited by many drugs that are co-administered to treat symptoms associated with diseases treated with thiopurines. For example, aspirin and other anti-inflammatory drugs, including olsalazine, sulfasalazine, and balsalazide, inhibit TPMT. The impact of TPMT genotypes on its methylating activity is well defined, and genotyping patients to identify TPMT metabolizer status is common clinical practice. Unfortunately, there has been no concerted effort to comprehensively identify drugs on the market that impact TPMT activity. The inhibition of TPMT by co-administered drugs could in part be responsible for idiosyncratic toxicities associated with thiopurine drug therapy. Methods: Here, we report a facile approach to produce large quantities of recombinant TPMT and a high-throughput assay that utilizes the shift in absorbance due to the methylation of thiopurines to report on TPMT activity. Results and Conclusions: With purified TPMT on hand and the absorbance activity assay, we confirmed several compounds that inhibit TPMT, and the results were comparable to a mass spectral assay that measured 6-MP methylation. Understanding the impact of co-administered drugs on TPMT activity will improve the safety and efficacy of thiopurine-based treatment regimens.
Surface Microstructure Enhanced Cryogenic Infrared Light Emitting Diodes for Semiconductor Broadband Upconversion
Authors: Bai Peng , Wang Hanbin , Lv Rongrong , Wang Yi , Li Yinqiao , Han Shangjie , Cai Jiaxuan , Yang Ning , Chu Weidong , Xie Yan , Chen Meng , Wang Yingxin , Zhao Ziran
Published: December 2024

Broadband upconversion has various applications in solar photovoltaic, infrared and terahertz detection imaging, and biomedicine. The low efficiency of the light-emitting diodes (LEDs) limits the broadband upconversion performance. In this paper, we propose to use surface microstructures to enhance the electroluminescence efficiency (ELE) of LEDs. Systematical investigations on the cryogenic-temperature performances of microstructure-coupled LEDs, including electroluminescence efficiency, luminescence spectrum, and recombination rate, have been carried out by elaborating their enhancement mechanism and light emitting characteristics both experimentally and theoretically. We have revealed that the reason for the nearly 35% ELE enhancement of the optimized structure under cryogenic temperature and weak injection current is the efficient carrier injection efficiency and the high recombination rate in the active region. We also compare studies of the surface luminescence uniformity of the optimized LED with that of the unoptimized device. This work gives a precise description, and explanation of the performance of the optimized microstructure coupled LED at low temperatures, providing important guidance and inspiration for the optimization of broadband upconverter in the cryogenic temperature region.
Development of Spatial Memory: A Behavioral Study
Authors: Kostakos Konstantinos , Pliakopanou Alexandra , Meimaridis Vasileios , Galanou Ourania-Natalia (Oriana) , Anagnostou Aikaterini Argyro , Sertidou Dimitra , Katis Panagiotis , Anastasiou Periklis , Katsoulidis Konstantinos , Lykogiorgos Yannis , Mytilinaios Dimitrios , Katsenos Andreas P. , Simos Yannis V. , Bellos Stefanos , Konitsiotis Spyridon , Peschos Dimitrios , Tsamis Konstantinos I.
Published: December 2024

Although spatial memory has been widely studied in rodents, developmental studies involving humans are limited in number and sample size. We designed and studied the validity of two simple experimental setups for the evaluation of spatial memory and navigation development. The dataset of this study was composed of 496 schoolchildren, from 4 to 15 years old. Participants were tested blindfolded on their ability to navigate in a square area between three stool stations while performing an item-collecting task, having observed the experimental space and procedure (Test 1) or having, in addition, executed the task open-eyed (Test 2). The performance times were analyzed to identify age-specific differences. Parametric methods, including the one-way ANOVA and independent samples t-test, were employed. Statistically significant differences were observed in the mean performance time among age groups, as well as within the same age groups when comparing Test 1 and Test 2. Our results revealed a performance improvement with aging for both functions and showed that spatial memory and spatial navigation develop throughout childhood and puberty and interact during development. When children integrate visual stimuli with other sensory inputs, they can form stronger spatial memories, thereby enhancing their navigation skills. The proposed experimental setup is considered feasible and can be used for behavioral studies of navigation-related memory in children and beyond with appropriate adaptations, allowing for large-scale assessment.
The Camden Study—A Pregnancy Cohort Study of Pregnancy Complications and Birth Outcomes in Camden, New Jersey, USA
Authors: Shiau Stephanie , Chen Xinhua , April-Sanders Ayana , Francis Ellen , Rawal Shristi , Hansel Megan , Adeyemi Kehinde , Rivera-Núñez Zorimar , Barrett Emily
Published: December 2024

Background: Pregnancy is a unique stage of the life course characterized by trade-offs between the nutritional, immune, and metabolic needs of the mother and fetus. The Camden Study was originally initiated to examine nutritional status, growth, and birth outcomes in adolescent pregnancies and expanded to study dietary and molecular predictors of pregnancy complications and birth outcomes in young women. Methods: From 1985–2006, 4765 pregnant participants aged 12 years and older were recruited from Camden, NJ, one of the poorest cities in the US. The cohort reflects a population under-represented in perinatal cohort studies (45% Hispanic, 38% non-Hispanic Black, 17% White participants; 98% using Medicaid in pregnancy). Study visits, including questionnaires, dietary assessments, and biospecimen collection, occurred in early and late pregnancy as well as at delivery. Medical records were abstracted, and a subset of mothers and infants participated in a six-week postpartum visit. Results: Findings from the Camden Study have added to the understanding of adolescent and young adult maternal health and perinatal outcomes. These include associations of adolescent linear growth while pregnant with smaller neonatal birth size, low dietary zinc intake in early pregnancy with increased risk of delivery <33 gestational weeks, and higher circulating fatty acid levels with greater insulin resistance. More recent analyses have begun to unpack the biochemical pathways in pregnancy that may be shaped by race as an indicator of systemic racism. Conclusions: The Camden Study data and biorepositories are well-positioned to support future research aimed at better understanding perinatal health in under-represented women and infants. Linkages to subsequent health and administrative records and the potential for recontacting participants over 18–39 years after initial participation may provide key insights into the trajectories of maternal and child health across the life course.
Nopalea cochenillifera Regulates the Immune Response and Gut Microbiota in Mice
Authors: Kozai Hana , Watanabe Chihiro , Kosaka Rina , Aoki Takafumi , Hamada Hiroki , Kawashima Masato , Kono Takumi , Akagi Kosuke , Kremenik Michael J. , Yano Hiromi , Tanaka Mamoru , Oyanagi Eri
Published: December 2024

Background: Cactus contains dietary fiber and minerals and is expected to have preventive effects against diabetes, arteriosclerosis, and other diseases. Additionally, cactus intake induces the production of short-chain fatty acids derived from the gut microbiota, which might influence immune functions. In this study, we examined the effects of a cactus (Nopalea cochenillifera: NC)-supplemented diet on lipopolysaccharide (LPS)-induced immune responses and intestinal barrier function. Methods: Male C3H/HeN mice were randomly divided into three groups—no fiber (NF), cellulose-containing fiber (Cellu), and cactus-added (NC) diets—for 6 weeks. The TNF-α and IL-10 responses to LPS, antibody titers, and intestinal barrier function, as well as the fecal microbiota, were analyzed. Results: The plasma TNF-α but not the IL-10 concentrations were significantly higher in the NC group than in the NF and Cellu groups. Furthermore, the plasma IgG antibody titers were significantly higher in the NC group than in the other groups. The NC group showed higher mucin content and IgA antibody titers in their feces compared with the Cellu group. The succinate and lactate contents, which induce a reduction in TNF-α secretion by macrophages, in the cecum of the NC group were significantly lower than those in the Cellu and NF groups. In contrast, the butyrate content was significantly higher in the cecum of the NC group compared to that of the Cellu group, with a significantly higher relative abundance of butyrate-producing bacteria. Conclusions: Taken together, we found that cactus intake regulates innate and adaptive immune function via the gut microbiota in mice. Therefore, cactus supplementation might serve as a strategy to develop novel functional foods with dietary fiber.
Status of Inappropriate Complementary Feeding and Its Associated Factors Among Infants of 9–23 Months
Authors: Ashraf Iqra , Bestman Prince L. , Assiri Abdullah A. , Kamal Ghulam Mustafa , Uddin Jalal , Luo Jiayou , Orayj Khalid M. , Ishaqui Azfar A.
Published: December 2024

Background: Inappropriate complementary feeding during the first two years of life significantly impacts children’s health, increasing risks of malnutrition and illness. Methods: This study investigates factors influencing early feeding patterns among 600 mothers of children aged 9–23 months in selected hospitals in Punjab, Pakistan. Using a structured questionnaire, data were collected and analyzed, with associations measured by odds ratios (ORs) and 95% confidence intervals (CIs). Results: The results showed the key indicators of inappropriate complementary feeding among young children, including timely complementary feeding, minimum meal frequency, dietary diversity, and acceptable diet. The rates for these factors were found to be 60.3%, 32.7%, 24.6%, and 48.5%, respectively. The study identified several significant factors influencing these practices. Key predictors of inappropriate feeding included the order of birth, the mother’s employment status, parental education, the number of children, household income, maternal knowledge, and maternal health. Conclusion: The findings underscore that maternal education, employment, and health significantly influence complementary feeding. Targeted interventions and education programs are essential to support healthy feeding behaviors, especially for mothers facing challenges related to education, work, or health conditions. Addressing these practices can improve child health outcomes, contributing to economic growth and a healthier future for Pakistan’s youngest population.
Assessing Prognosis: Factors Influencing Outcomes in Hospitalized Lung Cancer
Authors: Peña-López Jesús , Gutiérrez-Sainz Laura , Jiménez-Bou Diego , Ruíz-Gutiérrez Icíar , Navas-Jiménez Carmen , Alonso-Eiras Jorge Ignacio , García-Zamarriego Álvaro , Sánchez-Cabrero Darío , Ruíz-Giménez Leticia , Pertejo-Fernández Ana , Villamayor-Sánchez Julia , Cruz-Castellanos Patricia , Higuera-Gómez Oliver , de Castro Javier
Published: December 2024

Background: Patients with lung cancer experience higher rates of hospitalization due to their elevated mortality and associated comorbidities. Hospital admissions among oncology patients often indicate organ vulnerability and are linked to poor prognosis. This study aimed to assess the characteristics and potential prognostic factors of hospitalized lung cancer patients. Methods: We retrospectively analyzed 646 patients admitted from June 2021 to May 2022 to the Medical Oncology Service at La Paz University Hospital (Madrid, Spain). Results: During this period, 158 patients admitted had lung cancer (24.5%). The median overall survival since admission (mOSSA) was 3.3 months (95%CI: 1.86–7.74). In the univariate analysis, poorer mOSSA was associated with admission for tumor-related causes (1.33 vs. 7.30 months, p < 0.001), ECOG ≥ 2 (2.43 vs. 8.50 months, p < 0.001), NLR ≥ 6 (1.87 vs. 7.40 months), PNI ≤ 40 (1.67 vs. 4.97 months), and LDH ≥ 210 (2.27 vs. 7.87 months, p = 0.044). In the multivariate analysis, independent prognostic factors included admission for tumor-related causes (p = 0.032, aHR 1.81, 95%CI: 1.05–3.11) and ECOG ≥ 2 (p = 0.041, aHR 1.80, 95%CI: 1.03–3.16). Conclusions: Hospital admission for lung cancer is a poor prognostic event, particularly when associated with tumor-related causes or a decline in performance status.
Antimicrobial Resistance in the Context of Animal Production and Meat Products in Poland—A Critical Review and Future Perspective
Authors: Wiśniewski Patryk , Trymers Miłosz , Chajęcka-Wierzchowska Wioleta , Tkacz Katarzyna , Zadernowska Anna , Modzelewska-Kapituła Monika
Published: December 2024

The prevalence of antimicrobial-resistant bacteria in meat and meat products is a significant public health challenge, largely driven by the excessive and inappropriate use of antimicrobials in animal husbandry. In Poland, a key meat producer in Europe, antibiotic-resistant pathogens such as Campylobacter spp., Staphylococcus spp., Enterococcus spp., Listeria monocytogenes, and Enterobacterales have been detected in meat, posing serious risks to consumers. This review examines the use of antimicrobial agents in meat production and the resulting antimicrobial resistance (AMR) in microorganisms isolated from meat products in Poland. The mechanisms of AMR, genetic factors, and prevalence in Poland are presented. It highlights key factors contributing to AMR, such as antibiotic misuse in livestock farming, and discusses the legal regulations governing veterinary drug residues in food. This review emphasizes the importance of monitoring and enforcement to safeguard public health and calls for further research on AMR in the meat industry. Antimicrobial resistance in meat and meat products in Poland is a huge challenge, requiring stricter antibiotic controls in animal husbandry and improved surveillance systems. Additionally, the impact of husbandry practices on the environment and food requires further research. Future efforts should focus on nationwide monitoring, alternative strategies to reduce antibiotic use, and stronger enforcement to combat antimicrobial resistance and protect public health.
Drug Delivery Across the Blood–Brain Barrier: A New Strategy for the Treatment of Neurological Diseases
Authors: Jiao Yimai , Yang Luosen , Wang Rujuan , Song Guoqiang , Fu Jingxuan , Wang Jinping , Gao Na , Wang Hui
Published: December 2024

The blood–brain barrier (BBB) serves as a highly selective barrier between the blood and the central nervous system (CNS), and its main function is to protect the brain from foreign substances. This physiological property plays a crucial role in maintaining CNS homeostasis, but at the same time greatly limits the delivery of drug molecules to the CNS, thus posing a major challenge for the treatment of neurological diseases. Given that the high incidence and low cure rate of neurological diseases have become a global public health problem, the development of effective BBB penetration technologies is important for enhancing the efficiency of CNS drug delivery, reducing systemic toxicity, and improving the therapeutic outcomes of neurological diseases. This review describes the physiological and pathological properties of the BBB, as well as the current challenges of trans-BBB drug delivery, detailing the structural basis of the BBB and its role in CNS protection. Secondly, this paper reviews the drug delivery strategies for the BBB in recent years, including physical, biological and chemical approaches, as well as nanoparticle-based delivery technologies, and provides a comprehensive assessment of the effectiveness, advantages and limitations of these delivery strategies. It is hoped that the review in this paper will provide valuable references and inspiration for future researchers in therapeutic studies of neurological diseases.
Novel Cyclic Peptide–Drug Conjugate P6-SN38 Toward Targeted Treatment of EGFR Overexpressed Non-Small Cell Lung Cancer
Authors: Bazylevich Andrii , Miller Ayala , Tkachenko Iryna , Merlani Maia , Patsenker Leonid , Gellerman Gary , Lubin Bat Chen R.
Published: December 2024

Background/Objectives: Here, we report on the synthesis and biological evaluation of a novel peptide–drug conjugate, P6-SN38, which consists of the EGFR-specific short cyclic peptide, P6, and the Topo I inhibitor SN38, which is a bioactive metabolite of the anticancer drug irinotecan. Methods: SN38 is attached to the peptide at position 20 of the E ring’s tertiary hydroxyl group via a mono-succinate linker. Results: The developed peptide–drug conjugate (PDC) exhibited sub-micromolar anticancer activity on EGFR-positive (EGFR+) cell lines but no effect on EGFR-negative (EGFR−) cells. In vivo studies have shown that this PDC specifically accumulates in EGFR+ non-small cell lung cancer (NSCLC) xenografts and presents superior anticancer activity compared to the EGFR-specific antibody cetuximab (ErbituxTM) and free SN38. The 10 mg/kg dose of P6-SN38 in a side-by-side EGFR+/EGFR− xenograft shows eradication of the EGFR+ tumor with good tolerance, but no inhibition of tumor growth of the EGFR− counterpart. Conclusions: The PDC examined in this study was proven to be highly efficient for NSCLC, broadening its utilization for targeted cancer therapy in EGFR overexpressed cancers.
Gynoxys reinaldii Cuatrec. and Gynoxys pulchella (Kunth) Cass.: Chemical and Enantioselective Analyses of Two Unprecedented Essential Oils from Ecuador
Authors: Maldonado Yessenia E. , Rodríguez María del Carmen , Calvopiña Karyna , Malagón Omar , Cumbicus Nixon , Gilardoni Gianluca
Published: December 2024

This study presents the first chemical and enantioselective analyses of essential oils (EOs) derived from the leaves of two endemic species, Gynoxys reinaldii Cuatrec. and Gynoxys pulchella (Kunth) Cass., from Loja, Ecuador. The distillation yields, by weight of dry plant material, were 0.04 ± 0.007% for G. reinaldii and 0.03 ± 0.002% for G. pulchella. For both plants, the chemical analyses were conducted by GC-MS (qualitative) and GC-FID (quantitative), on two stationary phases of different polarity (5% phenyl-methylpolysiloxane and polyethylene glycol). The major components of G. reinaldii EO included germacrene D (22.3–22.1%), α-pinene (14.2–14.1%), and (E)-β-caryophyllene (13.6–14.5%). Similarly, G. pulchella EO was characterized by germacrene D (9.5–12.9%), caryophyllene oxide (7.2–6.7%), and n-tricosane (4.9% in both columns). The enantioselective analyses were carried out with two columns, based on 2,3-diacetyl-6-tert-butyldimethylsilyl-β-cyclodextrin and 2,3-diethyl-6-tert-butyldimethylsilyl-β-cyclodextrin, detecting nine chiral terpenes and terpenoids. In G. reinaldii EO, (1S,5S)-(−)-α-pinene, (1S,5S)-(−)-β-pinene, (1S,5S)-(−)-sabinene, (R)-(−)-α-phellandrene, and (R)-(−)-β-phellandrene were enantiomerically pure, whereas cis-linalool oxide, linalool, terpinene-4-ol, and germacrene D were non-racemic mixtures of enantiomers. In G. pulchella, only (R)-(−)-α-phellandrene was enantiomerically pure. The detection of enantiomerically pure compounds may provide insights into the biosynthetic pathways and potential bioactivities of these EOs.
Influence of Wild and Cultivated Environments on the Antioxidant and Medicinal Components of Rhodiola sachalinensis A. Boriss.
Authors: Wei Xin , Chang Qiu-Yang , Liu Yang , Hua He-Nian , Liu Ya-Nan , Tang Zhong-Hua , Mu Li-Qiang
Published: December 2024

Rhodiola sachalinensis A. Boriss., recognized for its significant medicinal potential, is increasingly threatened by overharvesting in wild habitats. This study aims to elucidate the phytochemical and pharmacological distinctions between wild and cultivated R. sachalinensis to support sustainable cultivation practices. Utilizing UPLC and GC-MS, we conducted a comprehensive analysis of primary metabolites (e.g., soluble sugars, amino acids) and secondary metabolites (e.g., phenolic compounds, flavonoids) in both root and aboveground tissues from wild and cultivated sources. Results revealed that habitat plays a critical role in metabolite composition, with wild R. sachalinensis roots showing notably higher antioxidant properties and concentrations of key secondary metabolites, including epigallocatechin, which are linked to the plant’s medicinal efficacy. Conversely, cultivated plants demonstrated elevated levels of primary metabolites, reflecting adaptation to nutrient-rich, soil-based growth environments. These findings underscore the pharmacological importance of environmental factors in optimizing R. sachalinensis for medicinal use and highlight essential considerations for its sustainable cultivation strategies.
Genetic Diversity and Evaluation of Agro-Morphological Traits in Lettuce Core Collection
Authors: Moon Suyun , Hur Onsook , Kim Seong-Hoon , Lee Yoonjung , Oh Hyeonseok , Yi Jungyoon , Ko Ho-Cheol , Woo Hee-Jong , Ro Nayoung , Na Young-Wang
Published: December 2024

Lettuce (Lactuca sativa) is a globally significant leafy vegetable, valued for both its economic and nutritional contributions. The efficient conservation and use of the lettuce germplasm are crucial for breeding and genetic improvement. This study examined the genetic diversity and population structure of a core collection of the lettuce germplasm using genotyping by sequencing (GBS). A total of 7136 high-quality single-nucleotide polymorphisms (SNPs) were identified across nine chromosomes. Population analysis through Bayesian clustering and discriminant analysis of principal components (DAPC) revealed three distinct genetic clusters. Cluster 2 exhibited the greatest genetic diversity (He = 0.29, I = 0.44), while Cluster 3 had high levels of inbreeding (F = 0.79). Agro-morphological trait evaluation further identified significant differences in leaf length, plant weight, and head height across clusters. These findings provide valuable insights into the genetic and phenotypic diversity of lettuce, facilitating the development of more robust breeding programs. Additionally, the core collection established in this study offers a representative subset of the lettuce germplasm for future genomic research and conservation efforts.
The Staining Susceptibility and Surface Roughness of Teeth Restored by Microabrasion and Resin Infiltration: An In Vitro Study
Authors: Inna Treetossatep , Krajangta Nantawan , Rakmanee Thanasak
Published: December 2024

This study assessed the susceptibility to staining and surface roughness of white-spot lesions (WSLs) treated with resin infiltration (RIT) and microabrasion (MA) under simulated aging through thermocycling in red wine. Seventy-eight extracted human premolars with artificial WSLs were divided into three groups: untreated WSLs (control), RIT-treated (ICON®, DMG), and MA-treated (Opalustre®, Ultradent). Each group was further split: one subgroup immersed in artificial saliva and the other thermocycled in red wine. The color change (∆E) and surface roughness (Ra) were measured before and after staining using a spectrophotometer and a non-contact profilometer. Thermocycling in red wine increased color change (ΔE) across all groups, with the highest values observed for MA (43.94 ± 3.57), followed by RIT (31.40 ± 4.89). Surface roughness (Ra) was highest in untreated WSLs (0.61 ± 0.18 µm) and lowest in RIT (0.15 ± 0.03 µm). While RIT and MA similarly improved WSL appearance, RIT exhibited superior smoothness. These findings suggest that RIT provides a more durable surface with reduced roughness, although staining susceptibility remains comparable to MA. Moderate positive correlation was found between ΔE and Ra, indicating that roughness is one of the factors influencing color changes.
Statistical and Block Copolymers of n-Dodecyl and Allyl Isocyanate via Titanium-Mediated Coordination Polymerization: A Route to Polyisocyanates with Improved Thermal Stability
Authors: Iatrou Maria , Katara Aikaterini , Klonos Panagiotis A. , Kyritsis Apostolos , Pitsikalis Marinos
Published: December 2024

Well-defined amorphous/semi-crystalline statistical copolymers of n-dodecyl isocyanate, DDIC, and allyl isocyanate, ALIC, were synthesized via coordination polymerization using the chiral half-titanocene complex CpTiCl2(O-(S)-2-Bu) as an initiator. In the frame of the terminal model, the monomer reactivity ratios of the statistical copolymers were calculated using both well-known linear graphical methods and the computer program COPOINT. The molecular and structural characteristics of the copolymers were also calculated. The thermal properties of these samples were studied by differential scanning calorimetry, DSC, measurements. The kinetics of the thermal decomposition of the statistical copolymers was studied by thermogravimetric analysis, TGA, and differential thermogravimetry, DTG, and the activation energy of this process was calculated by employing several theoretical models. Moreover, block copolymers with the structure P[DDIC-b-(DDIC-co-ALIC)] were synthesized by sequential addition of monomers and coordination polymerization methodologies. The samples were characterized by nuclear magnetic resonance, NMR, spectroscopy; size exclusion chromatography, SEC; and DSC. The thermal stability of the blocks was also studied by TGA and DTG and compared to the corresponding statistical copolymers, showing that the macromolecular architecture greatly affects the properties of the copolymers. A thiol-ene click post-polymerization reaction was performed to introduce aromatic groups along the polyisocyanate chain in order to improve the thermal stability of the parent polymers. Evidently, these statistical and block copolymers can be employed as precursors for the synthesis of novel polyisocyanate-based materials.
Effect of Degree of Substitution and Polymer Ratio on the Structure of Chitosan: Carboxymethyl Starch (Bio)Polyelectrolyte Complexes
Authors: Castro David , Brovina Valentina , Litvinov Mikhail , Podshivalov Aleksandr
Published: December 2024

In this work, three carboxymethyl starches (CMS) were obtained by the two-step reaction process of carboxymethylation with different degrees of substitution (0.16, 0.33, and 0.36). From these samples, (bio)polyelectrolyte complexes ((bio)PECs) were obtained with chitosan (Chit) by the mixing of individual solutions of polymers (0.25 wt.%) at different volume ratios. The effect of the biopolymer and ionized groups of z ratios, pH, and degree of substitution of CMS in the formation of PEC were evaluated by turbidimetry and dynamic light scattering. The results showed that increasing the amount of CMS samples (ratio of z) led to an increase in the efficiency of the formation of (bio)PEC using CMS with a high DS value. Using the turbidimetry method for the chitosan and CMS mixtures, it was observed that the formation of (bio)PEC is divided into four transition zones delimited by pH transition points, and the stoichiometric complexation (z = 1) is achieved at a pH that displayed morphological changes “pHmorph”, which is a single point for Chit:CMS 1, and for Chit:CMS 2 and Chit:CMS 3, this is a range of 4.9–6.4 and 4.3–6.4, respectively. Analysis of the structural properties of the structures of (bio)PECs by dynamic light scattering was characterized by monomodal distribution, and the main observed effect was associated with an increase in the value of Davg with an increase in the ratio of Chit:CMS.
Interface Engineering of Styrenic Polymer Grafted Porous Micro-Silicon/Polyaniline Composite for Enhanced Lithium Storage Anode Materials
Authors: Lee Yechan , Naikwade Mahesh , Lee Sang-Wha
Published: December 2024

Si anode materials are promising candidates for next-generation Li-ion batteries (LIBs) because of their high capacities. However, expansion and low conductivity result in rapid performance degradation. Herein, we present a facile one-pot method for pyrolyzing polystyrene sulfonate (PSS) polymers at low temperatures (≤400 °C) to form a thin carbonaceous layer on the silicon surface. Specifically, micron silicon (mSi) was transformed into porous mSi (por-mSi) by a metal-assisted chemical etching method, and a phenyl-based thin film derived from the thermolysis of PSS formed a strong Si–C/Si–O–C covalent bonding with the Si surface, which helped maintain stable cycle performance by improving the interfacial properties of mSi. Additionally, PSS-grafted por-mSi (por-mSi@PSS) anode was coated with polyaniline (PANI) for endowing additional electrical conductivity. The por-mSi@PSS/PANI anode demonstrated a high reversible capacity of ~1500 mAh g−1 at 0.1 A g−1 after 100 cycles, outperforming or matching the performance reported in recent studies. A thin double layer composed of phenyl moieties and a conductive PANI coating improved the stability of Si-based anodes and provided an effective pathway for Li+ ion transport to the Si interface, suggesting that polymer-modified Si anodes hold significant promise for advanced LIB applications.
Harnessing of Sunflower Stalks by Hydrolysis and Fermentation with Hansenula polymorpha to Produce Biofuels
Authors: Martínez-Cartas Mª Lourdes , Cuevas-Aranda Manuel , Sánchez Sebastián
Published: December 2024

A sequential valorization process of sunflower stalks was carried out using nitric acid (0.1–2 mol dm−3) as a hydrolytic agent and fermenting the hydrolysate of higher sugar concentration in the presence of the non-conventional yeast Hansenula polymorpha. Values reached for ethanol yield (0.25 g g−1) and xylitol yield (0.14 g g−1) were higher than those achieved after pretreatment with other acids in previous studies. The effect of acid treatment with nitric, phosphoric, and sulfuric acids on the separated solid fractions was evaluated to determine its potential use as solid biofuel by FTIR and SEM determinations. A significant loss of lignin and hemicellulose was found in the solid treated with nitric acid, while a higher HHV was obtained when pretreated with phosphoric acid (19.16 MJ kg−1) and sulfuric acid (19.12 MJ kg−1). A subsequent enzymatic hydrolysis of acid-pretreated solids showed that the nitric acid pretreatment increased the availability of glucose from the cellulose fraction to a greater extent than the other two acids, by reducing the hemicellulose fraction to 0.7% and the lignin fraction to 2.5%. This study shows that pretreatment of biomass with nitric acid leads to better fermentation results to obtain biofuels such as ethanol, which could be further increased by additional enzymatic hydrolysis, while pretreatment with the other two acids generates better solid fuels.
“Totenfunk”: Investigating the Techniques and Theologies of the Electronic Voice Phenomenon
Authors: von Sydow Rikard Friberg
Published: December 2024

In 1963, the artist Friedrich Jürgenson announced to the Swedish press that he had established contact with the deceased through messages delivered via radio or tape recorder. This proclamation sparked a debate regarding the authenticity of his recordings. Were the voices truly those of the dead? Jürgenson attracted both critics and supporters, initiating a movement that would reach its peak in the 1970s. This paper focuses on the technical and theological aspects of the electronic voice phenomenon (EVP). How did the movement explain the technique used to transmit messages from the realm of the dead to our world? What did the dead wish to communicate—what kinds of messages were conveyed? Who were the transmitting spirits, and how did they describe their realm? This investigation is conducted through an analysis of the texts written by practitioners of the electronic voice phenomenon regarding their encounters with these spirits.
STFNet: A Spatiotemporal Fusion Network for Forest Change Detection Using Multi-Source Satellite Images
Authors: Tan Yingjiao , Sun Kaimin , Wei Jinjiang , Gao Song , Cui Wei , Duan Yu , Liu Junyi , Zhou Wanghui
Published: December 2024

Forest resources have important ecological and environmental values, and monitoring forest changes using remote sensing images is essential for resource management and ecological protection. However, current forest change detection methods fail to simultaneously integrate fine spatial information with temporal dynamic data, making them susceptible to pseudo changes induced by seasonal factors. In this paper, we propose a forest change detection method called STFNet that integrates multi-source spatiotemporal information. By combining fine spatial details of high-resolution images with dynamic information from time-series images, STFNet enhances the accuracy of forest change detection, alleviating the problem of information fusion difficulties caused by inconsistent granularity in spatiotemporal spectral features from different sources. In STFNet, we propose a cross-attention-based temporal differential feature fusion module (CATFF) to capture spatiotemporal dependencies within time-series images and a multiresolution contextual differential feature fusion module (MCDF) to achieve efficient spatial contexture fusion across multiresolution images. To validate our method, we conduct experiments using Gaofen and Sentinel-2 satellite images. Experimental results demonstrate that STFNet achieves excellent performance with an F1-score of 87.65%, outperforming state-of-the-art methods by at least 2.02%. Our ablation study further confirms the effectiveness of our method in leveraging time-series information to detect forest changes and suppress seasonal interference.
Integrating Satellite Observations and Hydrological Models to Unravel Large TROPOMI Methane Emissions in South Sudan Wetlands
Authors: Albuhaisi Yousef A. Y. , van der Velde Ype , Pandey Sudhanshu , Houweling Sander
Published: December 2024

This study presents a comprehensive investigation of Methane (CH4) emissions in the wetlands of South Sudan, employing an integrated approach that combines TROPOMI satellite data, river altimetry, and hydrological model outputs. TROPOMI data show a strong increase in CH4 concentrations over the Sudd wetlands from 2018 to 2022. We quantify CH4 emissions using these data. We find a twofold emission increase from 2018 to 2019 (9.2 ± 2.4 Tg yr−1) to 2020 to 2022 (16.3 ± 3.3 Tg yr−1). River altimetry data analysis elucidates the interconnected dynamics of river systems and CH4 emissions. We identify correlations and temporal alignments across South Sudan wetlands catchments. Our findings indicate a clear signature of ENSO driving the wetland dynamics and CH4 emissions in the Sudd by altering precipitation patterns, hydrology, and temperature, leading to variations in anaerobic conditions conducive to CH4 production. Significant correlations are found between CH4 emissions and PCR-GLOBWB-simulated soil moisture dynamics, groundwater recharge, and surface water parameters within specific catchments, underscoring the importance of these parameters on the catchment scale. Lagged correlations were found between hydrological parameters and CH4 emissions, particularly with PCR-GLOBWB-simulated capillary rise. These correlations shed light on the temporal dynamics of this poorly studied and quantified source of CH4. Our findings contribute to the current knowledge of wetland CH4 emissions and highlight the urgency of addressing the complex interplay between hydrology and carbon dynamics in these ecosystems that play a critical role in the global CH4 budget.
Global Semantic Classification of Fluvial Landscapes with Attention-Based Deep Learning
Authors: Carbonneau Patrice E.
Published: December 2024

Rivers occupy less than 1% of the earth’s surface and yet they perform ecosystem service functions that are crucial to civilisation. Global monitoring of this asset is within reach thanks to the development of big data portals such as Google Earth Engine (GEE) but several challenges relating to output quality and processing efficiency remain. In this technical note, we present a new deep learning pipeline that uses attention-based deep learning to perform state-of-the-art semantic classification of fluvial landscapes with Sentinel-2 imagery accessed via GEE. We train, validate and test the network on a multi-seasonal and multi-annual dataset drawn from a study site that covers 89% of the Earth’s surface. F1-scores for independent test data not used in model training reach 92% for rivers and 96% for lakes. This is achieved without post-processing and significantly reduced computation times, thus making automated global monitoring of rivers achievable.
Supplementing Forage with Traditional Chinese Medicine Can Increase Microbial Protein Synthesis in Sheep
Authors: Liang Xi , Bi Xue , Sano Hiroaki , Jin Jin
Published: December 2024

Traditional Chinese medicine (TCM) encompasses a wide range of natural herbs and plants that are used to treat or prevent diseases or promote health. This study aimed to evaluate the effect of feeding a TCM formula extract on nitrogen (N) balance, microbial nitrogen supply (MNS), and plasma leucine kinetics in sheep. Six sheep were fed with mixed hay (Hay-diet) only or supplemented with 2% TCM (mixture of Astragalus root, Angelica root, and Atractylodes rhizome; TCM-diet) in a crossover design over two 21-day periods. An isotope dilution of [1-13C]leucine was used to measure the rate of plasma leucine turnover. Purine derivative (PD) excretion in urine was determined to estimate the MNS. The TCM-diet significantly increased N intake (p < 0.01) and N digestibility (p = 0.02) compared to the Hay-diet, with less N excretion (p = 0.02) in feces, while no significant difference was observed between diets in terms of N excretion in urine and N retention. Total PD excretion and MNS were higher (p < 0.01), as well as the turnover rate of plasma leucine tended to be higher (p = 0.06) with the supplementation of TCM. The present results suggested that the TCM formula could be considered as a potential feed additive for ruminant production.
Evaluating Bioflocculation Harvesting of Freshwater and Marine Microalgae Using Exopolysaccharides (EPSs) from Klebsiella sp.
Authors: Yuan Yicheng , Lu Jingxuan , Wang Quan
Published: December 2024

The rising global energy demand and environmental concerns associated with fossil fuels have intensified interest in sustainable biofuel sources, with microalgae emerging as a viable candidate due to its high biomass yield and efficient CO2 conversion. However, the economic feasibility of microalgal biofuels is currently challenged by costly harvesting processes. This study investigates the use of exopolysaccharides (EPSs) derived from Klebsiella sp. as an environmentally friendly bioflocculant for harvesting two microalgae species: Raphidocelis subcapitata and Dunaliella salina. Comparative flocculation experiments revealed that Klebsiella EPS promotes efficient aggregation in R. subcapitata, achieving over 90% flocculation efficiency, while performance with D. salina was impacted by high salinity, which reduced charge neutralization and bridging effects. Structural analyses using FTIR, 3D-EEM, CLSM, and XPS elucidated the EPS composition, underscoring the roles of polysaccharides and proteins in facilitating microalgal aggregation. The findings indicate that Klebsiella EPS offers a sustainable alternative to chemical flocculants, supporting eco-friendly biofuel production and potential applications in wastewater treatment. This approach provides insights into optimizing EPS-based flocculation for diverse environmental conditions, paving the way for more sustainable biomass recovery practices.
Assessing Accessibility of Transport and Universal Access in the City of Tshwane Using Expert Opinion
Authors: Duri Babra , Luke Rose
Published: December 2024

Universal design and access in transport are essential for ensuring the equal and independent mobility of people with disability and those with reduced mobility. Despite progressive legislation and policies, the implementation of universal design and access standards in transport systems remains inconsistent in the City of Tshwane. This study aims to assess the state of transport and universal access in the City of Tshwane using expert opinion. Transport experts were interviewed using a semi-structured interview guide to gather in-depth insights. Thematic analysis was applied to identify issues related to policy, infrastructure, and service delivery. Experts reported that while legislation supports universal access, implementation is inconsistent, particularly in informal transport sectors like minibus taxis. The findings reveal significant gaps between policy goals and implementation. The inconsistent application of legislation has resulted in new transport modes, such as the BRT systems and the Gautrain high-speed rail, being designed with universal access principles, while other modes lag behind in accessibility standards. An integrated transport system with universally designed infrastructure is recommended to improve transport access for people with disability and those with reduced mobility.
Revisiting Inclusion: An Exploration of Refugee-Led Education for Children with Special Educational Needs and Disabilities in Lebanon
Authors: Safarha Elnaz , Zakharia Zeena
Published: December 2024

This article explores the concept of inclusive education in contexts of forced displacement, where refugeehood intersects with special educational needs and disabilities (SEND), as well as gender, poverty, and overlapping forms of discrimination. Drawing on extensive engagement with a refugee-led, non-formal educational organization in Lebanon, we revisit inclusion for refugee children with SEND through a bottom-up lens. We consider inclusion within Lebanon’s sociopolitical landscape, focusing on a community of educators, most of whom are refugees themselves. Grounded in decolonial feminist epistemologies and critical refugee studies, we highlight the role of educators as cultural actors who employ engaged pedagogies to humanize the educational experiences of refugee children with SEND. By challenging traditional top-down, outcome-oriented policies that focus solely on structural access, this paper advocates for an alternative framework based on refugee educators’ orientations to working with children with SEND. This framework prioritizes holistic, context-sensitive approaches to inclusion and underscores the importance of humanizing education for refugees.
Stable or Unstable? Evaluating the Strength Outcomes of 12-Week Resistance Training in Youth Judo Athletes
Authors: Stanković Nemanja , Stupar Dušan , Ignjatović Aleksandar , Milošević Nikola , Trajković Nebojša
Published: December 2024

Background: The purpose of this study was to compare the effects of resistance training on stable versus unstable surfaces on strength performance in young judokas. Methods: The study included 18 young judokas (age: 13.2 ± 1.2 years) with 4.7 years of training experience assigned to either the URT (unstable resistance training) group or the STG (stable resistance training) group. Both groups performed the bench press and squat exercise for 12 weeks (3 sessions/week, 30–40 min each), with one group performing on the stable surface and the other on the unstable surface. The tests included the 1RM bench press and squat, maximal power output during bench press and squat (Pmax), abdominal strength test (AB60), and the standing long jump (SLJ). Results: Statistical analyses revealed a group × time interaction for AB60 (p < 0.02) in favor of the URT group. Significant main effects of time (p < 0.01) for the 1RM bench press, 1RM squat, bench press power, AB60, and SLJ were found. There were no significant effects for squat maximal power in both groups (p ˃ 0.05). Conclusions: Both unstable and stable resistance training effectively improved maximal strength and performance in adolescent judokas, with unstable training offering additional benefits in abdominal strength.
Exploring the Relationship Between Restorativeness, Environmental Risk Perception, and Well-Being: The Case Study of Piazzola sul Brenta
Authors: Miola Laura , Rinaldi Enrico , Di Resta Sara , Pazzaglia Francesca
Published: December 2024

Urban environments in Northeastern Italy, both natural and historical, are exposed to urbanization, climate change, and environmental risks that can impact perceptions of environmental quality and sustainability. However, the relationship between the perceived environmental risks, the related emotions, and the quality of urban living and well-being remains underexplored. The present study, therefore, aims to investigate the link between the perceived risk of adverse events and the emotion felt about the possibility of damage to historically and economically valuable sites, as well as the residents’ restorativeness, well-being, residential satisfaction, and sense of community. Participants completed online questionnaires addressing the following variables: perceived risk of environmental events; emotions of possible damage; restorativeness; well-being; residential satisfaction; and sense of community. The results showed negative relationships between perceived risk and residential satisfaction, well-being and restorativeness, but positive relationships between negative emotions of losing places of economic value and residential satisfaction, sense of community, and restorativeness. The findings highlight the role of emotions and restorativeness with well-being and satisfaction in residents of Piazzola sul Brenta (Italy).
Geo-Environmental Risk Assessment of Sand Dunes Encroachment Hazards in Arid Lands Using Machine Learning Techniques
Authors: Abd El Aal Ahmed K. , GabAllah Hossam M. , Megahed Hanaa A. , Selim Maha K. , Hegab Mahmoud A. , Fadl Mohamed E. , Rebouh Nazih Y. , El-Bagoury Heba
Published: December 2024

Machine Learning Techniques (MLTs) and accurate geographic mapping are crucial for managing natural hazards, especially when monitoring the movement of sand dunes. This study presents the integration of MLTs with geographic information systems (GIS) and “R” software to monitor sand dune movement in Najran City, Saudi Arabia (KSA). Utilizing Linear Support Vector Machine (SVM), Random Forest (RF), and Artificial Neural Networks (ANN) with nine dune-related variables, this study introduces a new Drifting Sand Index (DSI) for effectively identifying and mapping dune accumulations. The DSI incorporates multispectral sensors data and demonstrates a robust capability for monitoring sand dune dynamics. Field surveys and spatial data analysis were used to identify about 100 dune locations, which were then divided into training (70%) and validation (30%) sets at random. These models produced a thorough dune encroachment risk map that divided areas into five hazard zones: very low, low, medium, high, and very high risk. The results show an average sand dune movement of 0.8 m/year towards the southeast. Performance evaluation utilizing the Area Under Curve-Receiver Operating Characteristic (AUC-ROC) approach revealed AUC values of 96.2% for SVM, 94.2% for RF, and 93% for ANN, indicating RF (AUC = 96.2%) as the most effective MLTs. This crucial information provides valuable insights for sustainable development and environmental protection, enabling decision-makers to prioritize regions for mitigation techniques against sand dune encroachment.