bluuebunny/crossref_metadata_2025 huggingface dataset:
Productivity and Nutritional Trait Improvements of Different Tomatoes Cultivated with Effective Microorganisms Technology
Authors: Tommonaro Giuseppina , Abbamondi Gennaro Roberto , Nicolaus Barbara , Poli Annarita , D’Angelo Costantino , Iodice Carmine , De Prisco Rocco
Published: February 2021

The use of ecofriendly strategies, such as the use of Plant Growth Promoting Bacteria, to improve the yield and quality of crops has become necessary to satisfy the growing demand of food and to avoid the use of chemical fertilizers and pesticides. In this study, we report the effects of an innovative microbial inoculation technique, namely Effective Microorganisms (EM), compared with traditional approaches, on productivity and nutritional aspect of four tomato varieties: Brandywine, Corbarino Giallo, S. Marzano Cirio 3, S. Marzano Antico. Results showed an increase of plant productivity as well as an enhanced antioxidant activity mainly in San Marzano Antico and Brandywine varieties treated with EM technology. Moreover, the polyphenol and carotenoid contents also changed, in response to the plant treatments. In conclusion, the application of EM® technology in agriculture could represent a very promising strategy in agricultural sustainability.
Optimized Supply Chain Management of Rice in South Korea: Location–Allocation Model of Rice Production
Authors: An Kyunam , Kim Sumin , Shin Seoho , Min Hyunkyoung , Kim Sojung
Published: February 2021

Planning for optimized farming with the aim of providing ideal site and cultivar selection is critical for a stable and sustainable supply of rice with sufficient quantity and quality to customers. In this study, a range of morphological characteristics and yield of eight rice cultivars that are commonly cultivated in Korea were investigated from 2005 to 2020. All morphological characteristics were significantly different among the eight rice cultivars. The dataset of morphological characteristics and yield was used to isolate groups of similar rice cultivars. The k-means clustering method was used to group the rice cultivars. Three groups (Group 1, Group 2, and Group 3) were created. Most cultivars were in Group 1. High-yielding rice cultivars were in Group 2, while the rice cultivars in Group 3 had the lowest rice grain yield. After grouping these rice cultivars, ideal farming locations for all three rice cultivar groups were identified to reduce transportation cost using an optimized location–allocation model. Simulation results suggested the following: (1) Group 1 should be produced in Jellanam-do (south west region), (2) Group 2 should be produced in Chungcheongnam-do (central west region), and (3) Group 3 should be mainly produced in the central west region of South Korea. Simulation results showed the potential to reduce transportation cost by around 0.014%. This can also reduce 21.04 tons of CO2 emission from a freight truck. Because these eight cultivars only make up 19.76% of the total rice production in South Korea, the cost reduction proportion was only 0.014% of total revenue. In future studies, more rice cultivars should be investigated to increase the efficiency of the model performance.
Electrolyte and Acid-Base Disorders Triggered by Aminoglycoside or Colistin Therapy: A Systematic Review
Authors: Scoglio Martin , Bronz Gabriel , Rinoldi Pietro O. , Faré Pietro B. , Betti Céline , Bianchetti Mario G. , Simonetti Giacomo D. , Gennaro Viola , Renzi Samuele , Lava Sebastiano A. G. , Milani Gregorio P.
Published: February 2021

Aminoglycoside or colistin therapy may alter the renal tubular function without decreasing the glomerular filtration rate. This association has never been extensively investigated. We conducted a systematic review of the literature following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses recommendations. Databases searched included United States National Library of Medicine, Excerpta Medica, and Web of Science. For the final analysis, we evaluated 46 reports, published after 1960, describing 82 cases. A total of 286 electrolyte and acid-base disorders were reported. Hypomagnesemia, hypokalemia, and hypocalcemia were reported in more than three quarter of cases. Further disorders were, in decreasing order of frequency, metabolic alkalosis, hyponatremia, hypophosphatemia, hypouricemia, hypernatremia, and metabolic acidosis. Six electrolyte and acid-base disorders were reported in seven cases, five in 12 cases, four in 16 cases, three in 31 cases, two in 11 cases, and one in five cases. Laboratory features consistent with a loop of Henle/distal tubular dysfunction were noted in 56 (68%), with a proximal tubular dysfunction in three (3.7%), and with a mixed dysfunction in five (6.1%) cases. The laboratory abnormality was unclassified in the remaining 18 (22%) cases. Treatment with aminoglycosides or colistin may trigger a proximal tubular or, more frequently, a loop of Henle/distal tubular dysfunction.
Antioxidants for the Treatment of Breast Cancer: Are We There Yet?
Authors: Griñan-Lison Carmen , Blaya-Cánovas Jose L. , López-Tejada Araceli , Ávalos-Moreno Marta , Navarro-Ocón Alba , Cara Francisca E. , González-González Adrián , Lorente Jose A. , Marchal Juan A. , Granados-Principal Sergio
Published: February 2021

Breast cancer is the most frequent cancer and the leading cause of cancer death in women. Oxidative stress and the generation of reactive oxygen species (ROS) have been related to cancer progression. Compared to their normal counterparts, tumor cells show higher ROS levels and tight regulation of REDOX homeostasis to maintain a low degree of oxidative stress. Traditionally antioxidants have been extensively investigated to counteract breast carcinogenesis and tumor progression as chemopreventive agents; however, there is growing evidence indicating their potential as adjuvants for the treatment of breast cancer. Aimed to elucidate whether antioxidants could be a reality in the management of breast cancer patients, this review focuses on the latest investigations regarding the ambivalent role of antioxidants in the development of breast cancer, with special attention to the results derived from clinical trials, as well as their potential use as plausible agents in combination therapy and their power to ameliorate the side effects attributed to standard therapeutics. Data retrieved herein suggest that antioxidants play an important role in breast cancer prevention and the improvement of therapeutic efficacy; nevertheless, appropriate patient stratification based on “redoxidomics” or tumor subtype is mandatory in order to define the dosage for future standardized and personalized treatments of patients.
Platycodon grandiflorum Root Protects against Aβ-Induced Cognitive Dysfunction and Pathology in Female Models of Alzheimer’s Disease
Authors: Nam Yunkwon , Shin Soo Jung , Park Yong Ho , Kim Min-Jeong , Jeon Seong Gak , Lee Hyewon , Choi Yeji , Kim Tae-Jin , Shin Seong Min , Kim Jwa-Jin , Yoo Doo-Han , Kim Hyung Don , Kim Sujin , Moon Minho
Published: February 2021

Alzheimer’s disease (AD) is a devastating neurodegenerative disease characterized by irreversible cognitive dysfunction. Amyloid beta (Aβ) peptide is an important pathological factor that triggers the progression of AD through accumulation and aggregation, which leads to AD-related pathologies that consequently affect cognitive functions. Interestingly, several studies have reported that Platycodon grandiflorum root extract (PGE), besides exhibiting other bioactive effects, displays neuroprotective, anti-neuroinflammatory, and cognitive-enhancing effects. However, to date, it is not clear whether PGE can affect AD-related cognitive dysfunction and pathogenesis. Therefore, to investigate whether PGE influences cognitive impairment in an animal model of AD, we conducted a Y-maze test using a 5XFAD mouse model. Oral administration of PGE for 3 weeks at a daily dose of 100 mg/kg significantly ameliorated cognitive impairment in 5XFAD mice. Moreover, to elucidate the neurohistological mechanisms underlying the PGE-mediated alleviative effect on cognitive dysfunction, we performed histological analysis of hippocampal formation in these mice. Histopathological analysis showed that PGE significantly alleviated AD-related pathologies such as Aβ accumulation, neurodegeneration, oxidative stress, and neuroinflammation. In addition, we observed a neuroprotective and antioxidant effect of PGE in mouse hippocampal neurons. Our findings suggest that administration of PGE might act as one of the therapeutic agents for AD by decreasing Aβ related pathology and ameliorating Aβ induced cognitive impairment.
Native High-Density Lipoproteins (HDL) with Higher Paraoxonase Exerts a Potent Antiviral Effect against SARS-CoV-2 (COVID-19), While Glycated HDL Lost the Antiviral Activity
Authors: Cho Kyung-Hyun , Kim Jae-Ryong , Lee In-Chul , Kwon Hyung-Jun
Published: February 2021

Human high-density lipoproteins (HDL) show a broad spectrum of antiviral activity in terms of anti-infection. Although many reports have pointed out a correlation between a lower serum HDL-C and a higher risk of COVID-19 infection and progression, the in vitro antiviral activity of HDL against SARS-CoV-2 has not been reported. HDL functionality, such as antioxidant and anti-infection, can be impaired by oxidation and glycation and a change to pro-inflammatory properties. This study compared the antiviral activity of native HDL with glycated HDL via fructosylation and native low-density lipoproteins (LDL). After 72 h of fructosylation, glycated HDL showed a typical multimerized protein pattern with an elevation of yellowish fluorescence. Glycated HDL showed a smaller particle size with an ambiguous shape and a loss of paraoxonase activity up to 51% compared to native HDL. The phagocytosis of acetylated LDL was accelerated 1.3-fold by glycated HDL than native HDL. Native HDL showed 1.7 times higher cell viability and 3.6 times higher cytopathic effect (CPE) inhibition activity against SARS-CoV-2 than that of glycated HDL under 60 μg/mL (approximately final 2.2 μM) in a Vero E6 cell. Native HDL showed EC50 = 52.1 ± 1.1 μg/mL (approximately final 1.8 μM) for the CPE and CC50 = 79.4 ± 1.5 μg/mL (around 2.8 μM). The selective index (SI) of native HDL was calculated to be 1.52. In conclusion, native HDL shows potent antiviral activity against SARS-CoV-2 without cytotoxicity, while the glycation of HDL impairs its antiviral activity. These results may explain why patients with diabetes mellitus or hypertension are more sensitive to a COVID-19 infection and have a higher risk of mortality.
Improved Application of Hyperspectral Analysis to Rock Art Panels from El Castillo Cave (Spain)
Authors: Bayarri Vicente , Castillo Elena , Ripoll Sergio , Sebastián Miguel A.
Published: February 2021

Rock art is one of the most fragile and relevant cultural phenomena in world history, carried out in shelters or the walls and ceilings of caves with mineral and organic substances. The fact it has been preserved until now can be considered as fortunate since both anthropogenic and natural factors can cause its disappearance or deterioration. This is the reason why rock art needs special conservation and protection measures. The emergence of digital technologies has made a wide range of tools and programs available to the community for a more comprehensive documentation of rock art in both 2D and 3D. This paper shows a workflow that makes use of visible and near-infrared hyperspectral technology to manage, monitor and preserve this appreciated cultural heritage. Hyperspectral imaging is proven to be an efficient tool for the recognition of figures, coloring matter, and state of conservation of such valuable art.
Retrospective Case Series Regarding the Advantages of Cortico-Puncture (CP) Therapy in Association with Micro-Implant Assisted Rapid Palatal Expander (MARPE)
Authors: Bud Eugen-Silviu , Păcurar Mariana , Vlasa Alexandru , Lazăr Ana Petra , Lazăr Luminița , Vaida Petru , Bud Anamaria
Published: February 2021

Transverse maxillary deficiency currently affects 8–23% of adults. One of the most widely used orthodontic treatments today in patients with transverse maxillary defects is the maxillary skeletal expander (MSE). This was a retrospective observational imaging study regarding structural bone changes that may occur during healing after the placement of micro-implant assisted rapid palatal expanders (MARPE) in combination with cortico-puncture (CP) therapy. Regarding the magnitude of the mid-palatal suture opening, the mean split at the anterior nasal spine (ANS) and the posterior nasal spine (PNS) was 3.76 and 3.12 mm, respectively. The amount of split at the PNS was smaller than at the ANS, approximately 85% of the distance, showing that the opening of the midpalatal suture was almost parallel in the sagittal plane. On average, one-half of the anterior nasal spine (ANS) moved more than the contralateral by 0.89 mm. In the present study, we show that MARPE associated with CP therapy had a positive outcome on the midpalatal suture opening. This occurred in safe conditions, without post-surgery bleeding, and showing healing at the corticotomy level, with no signs of swelling or sepsis, which are side effects usually associated with more complex surgical treatments. Our results suggest that non-surgical palatal expansion, assisted by MARPE and CP, is achievable and predictable in young adults.
Flow Field Analysis and Feasibility Study of a Multistage Centrifugal Pump Designed for Low-Viscous Fluids
Authors: Shamsuddeen Mohamed Murshid , Ma Sang-Bum , Kim Sung , Yoon Ji-Hoon , Lee Kwang-Hee , Jung Changjun , Kim Jin-Hyuk
Published: February 2021

A multistage centrifugal pump is designed for pumping low-viscosity, highly volatile and flammable chemicals, including hydrocarbons, for high head requirements. The five-stage centrifugal pump consists of a double-suction impeller at the first stage followed by a twin volute. The impellers for stages two through five are single-suction impellers followed by diffuser vanes and return channel vanes. The analytical performance is calculated initially in the design stage by applying similarity laws to an existing scaled-down pump model designed for low flow rate applications. The proposed pump design is investigated using computational fluid dynamics tools to study its performance in design and off-design conditions for water as the base fluid. The design feasibility of the centrifugal pump is tested for other fluids, such as water at a high temperature and pressure, diesel and debutanized diesel. The pump design is found to be suitable for a variety of fluids and operating ranges. The losses in the pump are analyzed in each stage at the best efficiency point. The losses in efficiency and head are observed to be higher in the second stage than in other stages. The detailed flow behavior at the second stage is studied to identify the root cause of the losses. Design modifications are recommended to diminish the losses and improve the overall performance of the pump.
Effect of Pd on the Electrocatalytic Activity of Pt towards Oxidation of Ethanol in Alkaline Solutions
Authors: Jadali Salma , Kamyabi Mohammad Ali , Solla-Gullón José , Herrero Enrique
Published: February 2021

The understanding of electrocatalytic activity and poisoning resistance properties of Pt and Pd nanoparticles, recognized as the best electrocatalysts for the ethanol oxidation reaction, is an essential step for the commercialization of direct ethanol fuel cells (DEFCs). In this paper, mono and bimetallic Pt and Pd nanoparticles with different atomic ratios have been synthesized to study their electrocatalytic properties for an ethanol oxidation reaction in alkaline solutions. The different nanoparticles were physiochemically characterized by transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The electrochemical characterization was performed by cyclic voltammetry and chronoamperometry measurements. The electrochemical measurements indicate that Pt nanoparticles have much higher electrocatalytic activity for ethanol oxidation than Pd nanoparticles. The studies with bimetallic PtPd nanoparticles showed a significant impact of their composition on the ethanol oxidation. Thus, the highest electrocatalytic activity and poisoning resistance properties were obtained for Pt3Pd2 nanoparticles. Moreover, this study demonstrates that the poisoning of the catalyst surface through ethanol oxidation is related to the prevalence of the acetaldehyde–acetate route and the polymerization of acetaldehyde through aldol condensation in the alkaline media.
The Old Testament Prophecy of the Resurrection of the Dry Bones between the West and Byzantium
Authors: Planchette Yoanna
Published: February 2021

The imagery of the vision of the valley of dry bones (Ezek 37. 1–14) still fascinates theologians and historians of religion with its exegetical and liturgical significance. Rarely represented in medieval art, the iconography of this singular topic related to the Last Judgment deserves closer attention on the part of art historians. The aim of the present contribution is to remedy this situation by offering an analysis of the main pictorial representations of Ezekiel’s prophecy within the medieval East and West. This paper examines the evolution of the theme from the first pictorial evidence from Mesopotamia through the Roman late antique funerary sculpture into the Catalan and Germanic illuminated manuscript production from 11th and 12th centuries. Then, the field of the investigation broadens by taking into consideration the Byzantine artistic patterns of Ezekiel’s vision of the resurrection of the dead. Finally, this paper accents the multilayered contribution of the mural paintings from the Balkan cultural field. In order to reconsider this subject through the prism of the artistic interactions between East and West, the continuity of an ancient pictorial tradition that seems to have been previously neglected is highlighted.
Reanalysis Product-Based Nonstationary Frequency Analysis for Estimating Extreme Design Rainfall
Authors: Kim Dong-IK , Han Dawei , Lee Taesam
Published: February 2021

Nonstationarity is one major issue in hydrological models, especially in design rainfall analysis. Design rainfalls are typically estimated by annual maximum rainfalls (AMRs) of observations below 50 years in many parts of the world, including South Korea. However, due to the lack of data, the time-dependent nature may not be sufficiently identified by this classic approach. Here, this study aims to explore design rainfall with nonstationary condition using century-long reanalysis products that help one to go back to the early 20th century. Despite its useful representation of the past climate, the reanalysis products via observational data assimilation schemes and models have never been tested in representing the nonstationary behavior in extreme rainfall events. We used daily precipitations of two century-long reanalysis datasets as the ERA-20c by the European Centre for Medium-Range Weather Forecasts (ECMWF) and the 20th century reanalysis (20CR) by the National Oceanic and Atmospheric Administration (NOAA). The AMRs from 1900 to 2010 were derived from the grids over South Korea. The systematic errors were downgraded through quantile delta mapping (QDM), as well as conventional stationary quantile mapping (SQM). The evaluation result of the bias-corrected AMRs indicated the significant reduction of the errors. Furthermore, the AMRs present obvious increasing trends from 1900 to 2010. With the bias-corrected values, we carried out nonstationary frequency analysis based on the time-varying location parameters of generalized extreme value (GEV) distribution. Design rainfalls with certain return periods were estimated based on the expected number of exceedance (ENE) interpretation. Although there is a significant range of uncertainty, the design quantiles by the median parameters showed the significant relative difference, from −30.8% to 42.8% for QDM, compared with the quantiles by the multi-decadal observations. Even though the AMRs from the reanalysis products are challenged by various errors such as quantile mapping (QM) and systematic errors, the results from the current study imply that the proposed scheme with employing the reanalysis product might be beneficial to predict the future evolution of extreme precipitation and to estimate the design rainfall accordingly.
A Strategy to Elicit M2e-Specific Antibodies Using a Recombinant H7N9 Live Attenuated Influenza Vaccine Expressing Multiple M2e Tandem Repeats
Authors: Mezhenskaya Daria , Isakova-Sivak Irina , Kotomina Tatiana , Matyushenko Victoria , Kim Min-Chul , Bhatnagar Noopur , Kim Ki-Hye , Kang Sang-Moo , Rudenko Larisa
Published: February 2021

Influenza viruses remain a serious public health problem. Vaccination is the most effective way to prevent the disease; however, seasonal influenza vaccines demonstrate low or no effectiveness against antigenically drifted and newly emerged influenza viruses. Different strategies of eliciting immune responses against conserved parts of various influenza virus proteins are being developed worldwide. We constructed a universal live attenuated influenza vaccine (LAIV) candidate with enhanced breadth of protection by modifying H7N9 LAIV by incorporating four epitopes of M2 protein extracellular part into its hemagglutinin molecule. The new recombinant H7N9+4M2e vaccine induced anti-M2e antibody responses and demonstrated increased protection against heterosubtypic challenge viruses in direct and serum passive protection studies, compared to the classical H7N9 LAIV. The results of our study suggest that the H7N9+4M2e warrants further investigation in pre-clinical and phase 1 clinical trials.
Clinically Explored Virus-Based Therapies for the Treatment of Recurrent High-Grade Glioma in Adults
Authors: Immidisetti Amanda V. , Nwagwu Chibueze D. , Adamson David C. , Patel Nitesh V. , Carbonell Anne-Marie
Published: February 2021

As new treatment modalities are being explored in neuro-oncology, viruses are emerging as a promising class of therapeutics. Virotherapy consists of the introduction of either wild-type or engineered viruses to the site of disease, where they exert an antitumor effect. These viruses can either be non-lytic, in which case they are used to deliver gene therapy, or lytic, which induces tumor cell lysis and subsequent host immunologic response. Replication-competent viruses can then go on to further infect and lyse neighboring glioma cells. This treatment paradigm is being explored extensively in both preclinical and clinical studies for a variety of indications. Virus-based therapies are advantageous due to the natural susceptibility of glioma cells to viral infection, which improves therapeutic selectivity. Furthermore, lytic viruses expose glioma antigens to the host immune system and subsequently stimulate an immune response that specifically targets tumor cells. This review surveys the current landscape of oncolytic virotherapy clinical trials in high-grade glioma, summarizes preclinical experiences, identifies challenges associated with this modality across multiple trials, and highlights the potential to integrate this therapeutic strategy into promising combinatory approaches.
Dual Acting Carbon Monoxide Releasing Molecules and Carbonic Anhydrase Inhibitors Differentially Modulate Inflammation in Human Tenocytes
Authors: Gallorini Marialucia , Berardi Anna C. , Ricci Alessia , Antonetti Lamorgese Passeri Cristina , Zara Susi , Oliva Francesco , Cataldi Amelia , Carta Fabrizio , Carradori Simone
Published: February 2021

Sustained oxidative stress and inflammation have been reported as the major factors responsible for the failure of tendon healing during rotator cuff tears (RCTs) and rotator cuff disease (RCD). Although, their therapeutic management remains still challenging. Carbonic anhydrases (CAs) are involved in many pathological conditions, and the overexpression of both CA9 and 12 in inflamed joints has been recently reported. Consequently, a selective CA9/12 inhibition could be a feasible strategy for improving tendon recovery after injury. In addition, since carbon monoxide (CO) has been proven to have an important role in modulating inflammation, CO releasing molecules (CORMs) can be also potentially suitable compounds. The present study aims at evaluating five newly synthesized dual-mode acting CA inhibitors (CAIs)-CORMs compounds, belonging to two chemical scaffolds, on tendon-derived human primary cells under H2O2 stimulation in comparison with Meloxicam. Our results show that compounds 2 and 7 are the most promising of the series in counteracting oxidative stress-induced cytotoxicity and display a better profile in terms of enhanced viability, decreased LDH release, and augmented tenocyte proliferation compared to Meloxicam. Moreover, compound 7, as a potent superoxide scavenger, exerts its action inhibiting NF-ĸB translocation and downregulating iNOS, whereas compound 2 is more effective in increasing collagen I deposition. Taken together, our data highlight a potential role of CA in RCTs and RCD and the prospective effectiveness of compounds acting as CAI-CORM during inflammation.
cAMP Biosensors Based on Genetically Encoded Fluorescent/Luminescent Proteins
Authors: Kim Namdoo , Shin Seunghan , Bae Se Won
Published: February 2021

Cyclic adenosine monophosphate (cAMP) plays a key role in signal transduction pathways as a second messenger. Studies on the cAMP dynamics provided useful scientific insights for drug development and treatment of cAMP-related diseases such as some cancers and prefrontal cortex disorders. For example, modulation of cAMP-mediated intracellular signaling pathways by anti-tumor drugs could reduce tumor growth. However, most early stage tools used for measuring the cAMP level in living organisms require cell disruption, which is not appropriate for live cell imaging or animal imaging. Thus, in the last decades, tools were developed for real-time monitoring of cAMP distribution or signaling dynamics in a non-invasive manner. Genetically-encoded sensors based on fluorescent proteins and luciferases could be powerful tools to overcome these drawbacks. In this review, we discuss the recent genetically-encoded cAMP sensors advances, based on single fluorescent protein (FP), Föster resonance energy transfer (FRET), single luciferase, and bioluminescence resonance energy transfer (BRET) for real-time non-invasive imaging.
Brief Mindfulness Breathing Exercises and Working Memory Capacity: Findings from Two Experimental Approaches
Authors: Quek Frosch , Majeed Nadyanna , Kothari Meenakshi , Lua Verity , Ong Hee , Hartanto Andree
Published: February 2021

Mindfulness training has been extensively researched and found to elicit positive effects on cognitive performance, including working memory capacity. Benefits to cognitive function have been extended to brief mindfulness training as well. However, not much is known about its effect on working memory capacity. The current study examined the effects of a single 15-min session of mindful attention to breathing compared to a 15-min session of mind-wandering exercise in a within-subjects experimental design (Study 1; N = 82) and a between-subjects experimental design (Study 2; N = 424). Contrary to our hypotheses, in both experiments, we did not find any evidence that participants in the mindfulness condition outperform the control condition on an operation span task (Study 1) and a symmetry span task (Study 2). These results suggest that a single session of mindful practice may not be sufficient to enhance working memory capacity.
The Association between Executive Functions and Body Weight/BMI in Children and Adolescents with ADHD
Authors: Racicka-Pawlukiewicz Ewa , Kuć Katarzyna , Bielecki Maksymilian , Hanć Tomasz , Cybulska-Klosowicz Anita , Bryńska Anita
Published: February 2021

Despite the increasing body of research on Attention Deficit Hyperactivity Disorder (ADHD), the results of the studies assessing the relationship between executive function deficit and the risk of obesity in people with ADHD are incongruent. Our study aimed to assess the relationship between measures of executive functions and body weight and Body Mass Index (BMI) in children and adolescents with ADHD and control subjects. The study group consisted of 58 subjects aged from 8 to 17 years with ADHD. The Control group consisted of 62 healthy age and sex-matched participants from primary and secondary schools. Weight, height, and BMI measurements were standardized. The Sustained Attention to Response Test (SART) and the Attention Network Test (ANT) were used to assess executive functions. Based on the analysis of the correlation and analysis of moderation, we found that subjects with higher weight in the study group presented a lower efficiency of the inhibition processes and gave more impulsive and incorrect answers. The occurrence of impulsive reactions might contribute to the risk of excessive weight in children and adolescents with ADHD.
Systematic Review and Metanalysis of Oncomarkers in IPF Patients and Serial Changes of Oncomarkers in a Prospective Italian Real-Life Case Series
Authors: d’Alessandro Miriana , Bergantini Laura , Torricelli Elena , Cameli Paolo , Lavorini Federico , Pieroni Maria , Refini Rosa Metella , Sestini Piersante , Bargagli Elena
Published: February 2021

Background: Idiopathic pulmonary fibrosis (IPF) is a severe progressive interstitial lung disease. At 5-year follow-up, 15% of IPF patients develop lung cancer, which significantly reduces the survival rate. Here we review the literature on the clinical role of oncomarkers in IPF progression, and describe the trend of routine oncomarkers in IPF patients over the longest follow-up yet reported. Materials and methods: A systematic search of the literature in PubMed was performed to find relevant studies published up to 24 September 2020. The most common oncomarkers were chosen to select papers related to pulmonary fibrosis. Then, 24 IPF patients and 25 non-IPF patients, followed at Careggi ILD Referral Centre and Siena Regional Referral Centre for ILD, were enrolled consecutively. Results: A few studies reported an association between serum oncomarkers and severity of IPF. NSE, CEA, Ca19.9, and Ca125 were higher in the IPF, than in the non-IPF, group at every follow-up (p < 0.05). Ca15.3 concentrations were higher in the IPF, than the non-IPF, group at t3 (p = 0.0080) and t4 (p = 0.0168). To improve the specificity and sensitivity of Ca15.3, a panel of biomarkers was analyzed, with the IPF group as dependent variable, and chitotriosidase, Cyfra 21.1, Ca15.3, Ca125, and Ca19.9 as independent variables. Conclusions: This study focused on the discovery of multiple biomarker signatures, such as combinations of oncomarkers, that are widely and routinely available in biochemistry laboratories. The combination of clinical parameters and biological markers could help achieve more accurate results regarding prognosis and response to treatment in IPF. Our results could pave the way for a more “personalized” medical approach to patients affected by IPF.
Lipid Level, Lipid Variability, and Risk of Multiple Myeloma: A Nationwide Population-Based Study of 3,527,776 Subjects
Authors: Choi Taewoong , Choi In Young , Han Kyungdo , Jeong Su-Min , Yoo Jung Eun , Rhee Sang Youl , Park Yong-Gyu , Shin Dong Wook
Published: February 2021

(1) Background: There is evidence that abnormality in lipid metabolism promotes cancer development. This study investigated whether lipid level and its variability are associated with the development of MM at a population level. (2) Methods: A retrospective cohort study included a total of 3,527,776 subjects aged 40 and above who participated in ≥3 health examinations within the previous five years, including the index year (2012–2013). Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and triglyceride (TG) were measured, and visit-to-visit lipid variability were calculated by variability independent of the mean (VIM) method. The study population was followed from the health examination date in the index year until the diagnosis of MM, death, or the last follow-up date (31 December 2017). (3) Results: During a median (5–95%) 5.1 years of follow-up, 969 subjects developed MM. A lower risk of MM was observed with higher quartiles of baseline lipid levels compared to the lowest quartile group (Q4 vs. Q1: adjusted hazard ratios (aHRs) 0.51, 95% confidence interval (CI) (0.42–0.61) for TC; 0.50 (0.41–0.61) for HDL-C; 0.65 (0.54–0.77) for LDL-C; and 0.72 (0.60–0.87) for TG in model (3). Among all lipid measures, only variability in HDL-C was associated with risk of MM: aHRs (95% CI) were 1.12 (0.91–1.38), 1.19 (0.97–1.46), and 1.34 (1.09–1.65) in the Q2, Q3, and Q4, respectively, compared to the Q1 of VIM of HDL-C. (4) Conclusions: This study shows that patients with lower lipid levels and high HDL-C variability are at increased risk of developing MM.
The Role of Hypoxia in Glioblastoma Radiotherapy Resistance
Authors: Chédeville Agathe L. , Madureira Patricia A.
Published: February 2021

Glioblastoma (GB) (grade IV astrocytoma) is the most malignant type of primary brain tumor with a 16 months median survival time following diagnosis. Despite increasing attention regarding the development of targeted therapies for GB that resulted in around 450 clinical trials currently undergoing, radiotherapy still remains the most clinically effective treatment for these patients. Nevertheless, radiotherapy resistance (radioresistance) is commonly observed in GB patients leading to tumor recurrence and eventually patient death. It is therefore essential to unravel the molecular mechanisms underpinning GB cell radioresistance in order to develop novel strategies and combinational therapies focused on enhancing tumor cell sensitivity to radiotherapy. In this review, we present a comprehensive examination of the current literature regarding the role of hypoxia (O2 partial pressure less than 10 mmHg), a main GB microenvironmental factor, in radioresistance with the ultimate goal of identifying potential molecular markers and therapeutic targets to overcome this issue in the future.
Role of Methylation in Pro- and Anti-Cancer Immunity
Authors: Mehdi Ali , Rabbani Shafaat A.
Published: February 2021

DNA and RNA methylation play a vital role in the transcriptional regulation of various cell types including the differentiation and function of immune cells involved in pro- and anti-cancer immunity. Interactions of tumor and immune cells in the tumor microenvironment (TME) are complex. TME shapes the fate of tumors by modulating the dynamic DNA (and RNA) methylation patterns of these immune cells to alter their differentiation into pro-cancer (e.g., regulatory T cells) or anti-cancer (e.g., CD8+ T cells) cell types. This review considers the role of DNA and RNA methylation in myeloid and lymphoid cells in the activation, differentiation, and function that control the innate and adaptive immune responses in cancer and non-cancer contexts. Understanding the complex transcriptional regulation modulating differentiation and function of immune cells can help identify and validate therapeutic targets aimed at targeting DNA and RNA methylation to reduce cancer-associated morbidity and mortality.
The Catalytic Activity of Carbon-Supported Cu(I)-Phosphine Complexes for the Microwave-Assisted Synthesis of 1,2,3-Triazoles
Authors: Librando Ivy L. , Mahmoud Abdallah G. , Carabineiro Sónia A. C. , Guedes da Silva M. Fátima C. , Geraldes Carlos F. G. C. , Pombeiro Armando J. L.
Published: February 2021

A set of Cu(I) complexes with 3,7-diacetyl-1,3,7-triaza-5-phosphabicyclo-[3.3.1]nonane (DAPTA) phosphine ligands viz. [CuX(κP-DAPTA)3] (1: X = Br; 2: X = I) and [Cu(μ-X)(κP-DAPTA)2]2 (3: X = Br; 4: X = I) were immobilized on activated carbon (AC) and multi-walled carbon nanotubes (CNT), as well as on these materials after surface functionalization. The immobilized copper(I) complexes have shown favorable catalytic activity for the one-pot, microwave-assisted synthesis of 1,2,3-triazoles via the azide-alkyne cycloaddition reaction (CuAAC). The heterogenized systems with a copper loading of only 1.5–1.6% (w/w relative to carbon), established quantitative conversions after 15 min, at 80 °C, using 0.5 mol% of catalyst loading (relative to benzyl bromide). The most efficient supports concerning heterogenization were CNT treated with nitric acid and NaOH, and involving complexes 2 and 4 (in the same order, 2_CNT-ox-Na and 4_CNT-ox-Na). The immobilized catalysts can be recovered and recycled by simple workup and reused up to four consecutive cycles although with loss of activity.
Aftertreatment DeNOx Systems for Future Light Duty Lean-Burned Emission Regulations
Authors: Pereda-Ayo Beñat , González-Velasco Juan Ramon
Published: February 2021

Future light duty vehicles in Europe and the United States are required to be certified, owing to progressively more and more stringent regulations [...]
Information and Communication Technologies to Support Early Screening of Autism Spectrum Disorder: A Systematic Review
Authors: Desideri Lorenzo , Pérez-Fuster Patricia , Herrera Gerardo
Published: February 2021

The aim of this systematic review is to identify recent digital technologies used to detect early signs of autism spectrum disorder (ASD) in preschool children (i.e., up to six years of age). A systematic literature search was performed for English language articles and conference papers indexed in Pubmed, PsycInfo, ERIC, CINAHL, WoS, IEEE, and ACM digital libraries up until January 2020. A follow-up search was conducted to cover the literature published until December 2020 for the usefulness and interest in this area of research during the Covid-19 emergency. In total, 2427 articles were initially retrieved from databases search. Additional 481 articles were retrieved from follow-up search. Finally, 28 articles met the inclusion criteria and were included in the review. The studies included involved four main interface modalities: Natural User Interface (e.g., eye trackers), PC or mobile, Wearable, and Robotics. Most of the papers included (n = 20) involved the use of Level 1 screening tools. Notwithstanding the variability of the solutions identified, psychometric information points to considering available technologies as promising supports in clinical practice to detect early sign of ASD in young children. Further research is needed to understand the acceptability and increase use rates of technology-based screenings in clinical settings.
A Structured Approach to Skin and Soft Tissue Infections (SSTIs) in an Ambulatory Setting
Authors: Silverberg Benjamin
Published: February 2021

The skin is the largest, and arguably, the most vulnerable organ in the human body. Scratches and scrapes, bites and puncture wounds, impetigo and erysipelas—all these disruptions can lead to pain, swelling, and/or systemic symptoms. In this article, which is based on the Infectious Diseases Society of America’s 2014 guidelines and the World Society of Emergency Surgery and Surgical Infection Society of Europe’s 2018 consensus statement, a structured approach to skin and soft tissue infections (SSTIs) is reviewed, comparing treatment for suppurative and non-suppurative infections, and then discussing specific conditions commonly seen in Primary Care and Urgent Care facilities.
USH2A-Related Retinitis Pigmentosa: Staging of Disease Severity and Morpho-Functional Studies
Authors: Falsini Benedetto , Placidi Giorgio , De Siena Elisa , Savastano Maria Cristina , Minnella Angelo Maria , Maceroni Martina , Midena Giulia , Ziccardi Lucia , Parisi Vincenzo , Bertelli Matteo , Maltese Paolo Enrico , Chiurazzi Pietro , Rizzo Stanislao
Published: February 2021

Usher syndrome type 2A (USH2A) is a genetic disease characterized by bilateral neuro-sensory hypoacusia and retinitis pigmentosa (RP). While several methods, including electroretinogram (ERG), describe retinal function in USH2A patients, structural alterations can be assessed by optical coherence tomography (OCT). According to a recent collaborative study, RP can be staged considering visual acuity, visual field area and ellipsoid zone (EZ) width. The aim of this study was to retrospectively determine RP stage in a cohort of patients with USH2A gene variants and to correlate the results with age, as well as additional functional and morphological parameters. In 26 patients with established USH2A genotype, RP was staged according to recent international standards. The cumulative staging score was correlated with patients’ age, amplitude of full-field and focal flicker ERGs, and the OCT-measured area of sub-Retinal Pigment Epithelium (RPE) illumination (SRI). RP cumulative score (CS) was positively correlated (r = 0.6) with age. CS was also negatively correlated (rho = −0.7) with log10 ERG amplitudes and positively correlated (r = 0.5) with SRI. In USH2A patients, RP severity score is correlated with age and additional morpho-functional parameters not included in the international staging system and can reliably predict their abnormality at different stages of disease.
A Metafrontier Analysis on the Performance of Grain-Producing Regions in Norway
Authors: Alem Habtamu
Published: February 2021

Previous application of the stochastic frontier model and subsequent measurement of the performance of the crop sector can be criticized for the estimated production function relying on the assumption that the underlying technology is the same for different agricultural systems. This paper contributes to estimating regional efficiency and the technological gap in Norwegian grain farms using the stochastic metafrontier approach. For this study, we classified the country into regions with district level of development and, hence, production technologies. The dataset used is farm-level balanced panel data for 19 years (1996–2014) with 1463 observations from 196 family farms specialized in grain production. The study used the true random effect model and stochastic metafrontier analysis to estimate region level technical efficiency (TE) and technology gap ratio (TGR) in the two main grain-producing regions of Norway. The result of the analysis shows that farmers differ in performance and technology use. Consequently, the paper gives some regionally and farming system-based policy insights to increase grain production in the country to achieve self-sufficiency and small-scale farming in all regions.
Microgrids Power Quality Enhancement Using Model Predictive Control
Authors: Garcia-Torres Felix , Vazquez Sergio , Moreno-Garcia Isabel M. , Gil-de-Castro Aurora , Roncero-Sanchez Pedro , Moreno-Munoz Antonio
Published: February 2021

In electric power systems, any deviation with respect to the theoretical sinusoidal waveform is considered to be a disturbance in the power quality of the electrical grid. The deviation can alter any of the parameters of the waveform: frequency, amplitude, and symmetry among phases. Microgrid, as a part of the electric power system, has to contribute providing an adequate current waveform in grid connected-mode, as well as to guarantee similar voltage features than the standard requirement given for public distribution grids under normal exploitation conditions in islanded mode. Adequate power quality supply is necessary for the correct compatibility between all the devices connected to the same grid. In this paper, the power quality of microgrids is managed using a Model Predictive Control (MPC) methodology which regulates the power converters of the microgrids in order to achieve the requirements. The control algorithm is developed for the following microgrids working modes: grid-connected, islanded, and interconnected. The simulation results demonstrate that the proposed methodology improves the transient response in comparison with classical methods in all the working modes, minimizing the harmonic content in the current and the voltage even with the presence of non-balanced and non-harmonic-free three-phase voltage and current systems.
Bi-Directional Pyramid Network for Edge Detection
Authors: Li Kai , Tian Yingjie , Wang Bo , Qi Zhiquan , Wang Qi
Published: February 2021

Multi-scale representation plays a critical role in the field of edge detection. However, most of the existing research focuses on one of two aspects: fast training and accurate testing. In this paper, we propose a novel multi-scale method to resolve the balance between them. Specifically, according to multi-stream structures and the image pyramid principle, we construct a down-sampling pyramid network and a lightweight up-sampling pyramid network to enrich the multi-scale representation from the encoder and decoder, respectively. Next, these two pyramid networks and a backbone network constitute our overall architecture, a bi-directional pyramid network (BDP-Net). Extensive experiments show that compared with the state-of-the-art model, our method could improve the training speed by about one time while retaining a similar test accuracy. Especially, under the single-scale test, our approach also reaches human perception (F1 score of 0.803) on the BSDS500 database.
A Reference Model Assisted Adaptive Control Structure for Maglev Transportation System
Authors: Dalwadi Nihal , Deb Dipankar , Muyeen S. M.
Published: February 2021

Maglev transportation system is become a hot topic for researchers because of the distinctive advantages, such as frictionless motion, low power consumption, less noise, and being environmentally friendly. The maglev transportation system’s performance gets sufficiently influenced by the control method and the magnetic levitation system’s dynamic performance, which is a critical component of the maglev transportation system. The Magnetic Levitation System (MLS) is a group of unstable, nonlinear, uncertain, and electromagnetically coupled practical application. Control objective of this study is to design a position stabilizing control strategy for Magnetic Levitation system under extreme uncertain parametric conditions using a reference model governed by a reference stabilizer and nonlinear adaptive control structure. After successful tuning the reference stabilizer with and without time-varying payload disturbance, the tracking-error dynamics are obtained in the presence of both matched and mismatched types of parametric uncertainties. Next, the close-loop stability theorem is formulated for Lyapunov stability analysis to get the design constraints, parameter update laws, and adaptive control law. Numerical simulations performed for a high range of parametric violations check the control design’s efficacy. The performance robustness gets confirmed by comparing the results with the nonlinear control approach. The MLS gets performance recovery and settles within safe limits in few seconds using the proposed methodology. However, the nonlinear controller faces permanent failure in stabilizing the MLS.