bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential Molecular Biomarkers of Central Nervous System Damage in Breast Cancer Survivors
Authors: Pospelova Maria , Krasnikova Varvara , Fionik Olga , Alekseeva Tatyana , Samochernykh Konstantin , Ivanova Nataliya , Trofimov Nikita , Vavilova Tatyana , Vasilieva Elena , Topuzova Maria , Chaykovskaya Alexandra , Makhanova Albina , Mikhalicheva Anna , Bukkieva Tatyana , Restor Kenneth , Combs Stephanie , Shevtsov Maxim
Published: February 2022

Damage of the central nervous system (CNS), manifested by cognitive impairment, occurs in 80% of women with breast cancer (BC) as a complication of surgical treatment and radiochemotherapy. In this study, the levels of ICAM-1, PECAM-1, NSE, and anti-NR-2 antibodies which are associated with the damage of the CNS and the endothelium were measured in the blood by ELISA as potential biomarkers that might reflect pathogenetic mechanisms in these patients. A total of 102 patients enrolled in this single-center trial were divided into four groups: (1) 26 patients after breast cancer treatment, (2) 21 patients with chronic brain ischemia (CBI) and asymptomatic carotid stenosis (ICA stenosis) (CBI + ICA stenosis), (3) 35 patients with CBI but without asymptomatic carotid stenosis, and (4) 20 healthy female volunteers (control group). Intergroup analysis demonstrated that in the group of patients following BC treatment there was a significant increase of ICAM-1 (mean difference: −368.56, 95% CI −450.30 to −286.69, p < 0.001) and PECAM-1 (mean difference: −47.75, 95% CI −68.73 to −26.77, p < 0.001) molecules, as compared to the group of healthy volunteers. Additionally, a decrease of anti-NR-2 antibodies (mean difference: 0.89, 95% CI 0.41 to 1.48, p < 0.001) was detected. The intergroup comparison revealed comparable levels of ICAM-1 (mean difference: −33.58, 95% CI −58.10 to 125.26, p = 0.76), PECAM-1 (mean difference: −5.03, 95% CI −29.93 to 19.87, p = 0.95), as well as anti-NR-2 antibodies (mean difference: −0.05, 95% CI −0.26 to 0.16, p = 0.93) in patients after BC treatment and in patients with CBI + ICA stenosis. The NSE level in the group CBI + ICA stenosis was significantly higher than in women following BC treatment (mean difference: −43.64, 95% CI 3.31 to −83.99, p = 0.03). Comparable levels of ICAM-1 were also detected in patients after BC treatment and in the group of CBI (mean difference: −21.28, 95% CI −111.03 to 68.48, p = 0.92). The level of PECAM-1 molecules in patients after BC treatment was also comparable to group of CBI (mean difference: −13.68, 95% CI −35.51 to 8.15, p = 0.35). In conclusion, among other mechanisms, endothelial dysfunction might play a role in the damage of the CNS in breast cancer survivors.
Modification of Corneal Biomechanics and Intraocular Pressure Following Non-Penetrating Deep Sclerectomy
Authors: Díaz-Barreda María Dolores , Sánchez-Marín Ignacio , Boned-Murillo Ana , Pérez-Navarro Itziar , Martínez Juana , Pardina-Claver Elena , Pérez Diana , Ascaso Francisco Javier , Ibáñez Juan
Published: February 2022

Changes in the cornea can influence outcomes in patients with primary open-angle glaucoma (POAG). We aimed to evaluate the relevance of changes in corneal biomechanics and intraocular pressure (IOP) in patients undergoing non-penetrating deep sclerectomy (NPDS) with the Esnoper V2000 implant® (AJL Ophthalmic S.A., Gasteiz, Spain). We included 42 eyes of 42 patients with POAG scheduled for NPDS with the Esnoper V2000 implant. Biomechanical properties were measured by Ocular Response Analyzer® G3 (ORA; Reichert Inc., Depew, NY, USA). Corneal hysteresis (CH), corneal resistance factor (CRF), corneal compensated IOP (IOPcc), and Goldmann-correlated IOP (IOPg) were measured the day before surgery and on day 1, 7, and 30 and 2 and 3 months after surgery. CH initially increased, fell below the presurgical value at 30 days after the surgery, and increased again at 2 and 3 months. CRF, IOPcc, and IOPg decreased on the first day after surgery, then followed a trend of increasing but stayed below pre-surgery levels. All values reached statistical significance. While observed changes in corneal biomechanics after NPDS and Esnoper V2000 implant were significant, more studies are needed if we are to understand their influence on corneal biomechanics and their clinical relevance in POAG.
Influence of Belatacept- vs. CNI-Based Immunosuppression on Vascular Stiffness and Body Composition
Authors: Heleniak Zbigniew , Illersperger Sarah , Naik Marcel G. , Osmanodja Bilgin , Ronicke Simon , Eleftheriadis Georgios , Halleck Fabian , Budde Klemens
Published: February 2022

Background: Arterial stiffness and phase angle (PhA) have gained importance as a diagnostic and prognostic parameter in the management of cardiovascular disease. There are few studies regarding the differences in arterial stiffness and body composition between renal transplant recipients (RTRs) receiving belatacept (BELA) vs. calcineurin inhibitors (CNI). Therefore, we investigated the differences in arterial stiffness and body composition between RTRs treated with different immunosuppressants, including BELA. Methods: In total, 325 RTRs were enrolled in the study (mean age 52.2 years, M −62.7%). Arterial stiffness was determined with an automated oscillometric device. All body composition parameters were assessed, based on bioelectrical impedance analysis (BIA), and laboratory parameters were obtained from the medical files of the patients. Results: We did not detect any significant difference in terms of arterial stiffness and PhA in RTRs undergoing different immunosuppressive regimens, based on CsA, Tac, or BELA. Age was an essential risk factor for greater arterial stiffness. The PhA was associated with age, BMI, time of dialysis before transplantation, and kidney graft function. Conclusion: No significant differences in arterial stiffness and PhA were observed in RTRs under different immunosuppressive regimens. While our data provide additional evidence for arterial stiffness and PhA in RTRs, more research is needed to fully explore these cardiovascular risk factors and the impact of different immunosuppressive regimens.
Oncofertility and COVID-19: At the Crossroads between Two Time-Sensitive Fields
Authors: Varlas Valentin Nicolae , Borș Roxana Georgiana , Pop Anca Lucia , Năsui Bogdana Adriana , Bacalbasa Nicolae , Bohîlțea Roxana , Vlădăreanu Radu , Manolea Corina
Published: February 2022

Background: COVID-19 infection has dominated our lives and left its mark on it. The impact on fertility is major, and the long-term consequences may be disastrous. When we talk about oncofertility, we are talking about those patients worried about the delay in receiving medical services (possible cancelation of surgery, decreased availability of medical services, reorientation of medical resources) due to COVID-19. Finally, patients’ worsening biological and reproductive statuses, associated with high levels of anxiety and depression, are closely related to social restrictions, economic impact, reorientation of medical resources, health policies, and fears of SARS-CoV-2 infection. Aim: We reviewed the current literature on fertility during the COVID-19 pandemic and its effect on cancer patients. Specifically, how cancer treatment can affect fertility, the options to maintain fertility potential, and the recovery options available after treatment are increasingly common concerns among cancer patients. Methods: A systematic literature search was conducted using two main central databases (PubMed®/MEDLINE, and Web of Science) to identify relevant studies using keywords SARS-CoV-2, COVID-19, oncofertility, young cancer patient, cryopreservation, assisted reproductive techniques (ART), psychosocial, telemedicine. Results: In the present study, 45 papers were included, centered on the six main topics related to COVID-19. Conclusions: Fertility preservation (FP) should not be discontinued, but instead practiced with adjustments to prevent SARS-CoV-2 transmission. The increased risk of SARS-CoV-2 infection in cancer patients requires screening for COVID-19 before FP procedures, among both patients and medical staff in FP clinics, to prevent infection that would rapidly worsen the condition and lead to severe complications.
Surgical and Patient-Reported Outcomes of Delayed Anastomotic Urethroplasty for Male Pelvic Fracture Urethral Injury at a Japanese Referral Center
Authors: Horiguchi Akio , Shinchi Masayuki , Ojima Kenichiro , Hirano Yusuke , Ito Keiichi , Azuma Ryuichi
Published: February 2022

We aimed to assess the surgical and patient-reported outcomes of delayed anastomotic urethroplasty (DAU) for pelvic fracture urethral injury (PFUI). We included 211 male patients who underwent DAU for PFUI. DAU success was considered when the urethral lumen was sufficiently large for the passage of a flexible cystoscope, without additional treatment required. The patients completed the lower urinary tract symptoms (LUTS)-related quality of life (QOL) questionnaire (scores: 0, not at all; 1, a little; 2, somewhat; 3, a lot), EuroQol-5 dimensions (EQ-5D), and EQ-5D visual analog scale (EQ-VAS). Postoperative overall satisfaction was evaluated using the following responses: “very satisfied,” “satisfied,” “unsatisfied,” or “very unsatisfied.” DAU was successful in 95.3% cases, with a median postoperative follow-up duration of 48 months. Multivariate logistic regression analysis revealed that “greater blood loss” was an independent predictor of failed urethroplasty. Questionnaire responses were obtained from 80.1% patients. The mean LUTS-related QOL, EQ-5D score and EQ-VAS improved significantly from 2.8, 0.63 and 54.4 at baseline to 0.9, 0.81 and 76.6 postoperatively (p < 0.0001 for all parameters). Moreover, 35.5% and 59.2% of the patients responded being “satisfied” and “very satisfied,” respectively, with their DAU outcomes. DAU not only had a high surgical success rate, but also a significant beneficial effect on both LUTS-related QOL and overall health-related QOL.
Arrhythmogenic Right Ventricular Cardiomyopathy and Differential Diagnosis with Diseases Mimicking Its Phenotypes
Authors: Molitor Nadine , Duru Firat
Published: February 2022

Arrhythmogenic right ventricular cardiomyopathy (ARVC) is an inherited heart muscle disease, which is characterized by fibro-fatty replacement of predominantly the right ventricle (RV). The disease can result in ventricular tachyarrhythmias and sudden cardiac death. Our understanding of the pathophysiology and clinical expressivity of ARVC has been continuously evolving. The diagnosis can be challenging due to its variable expressivity, incomplete penetrance and the lack of specific diagnostic criteria. Idiopathic RV outflow tract tachycardia, Brugada Syndrome, athlete’s heart, dilated cardiomyopathy, myocarditis, cardiac sarcoidosis, congenital aneurysms and diverticula may mimic clinical phenotypes of ARVC. This review aims to provide an update on the differential diagnosis of ARVC.
Experimental and Numerical Analysis of SMC Compression Molding in Confined Regions—A Comparison of Simulation Approaches
Authors: Rothenhäusler Florian , Meyer Nils , Wehler Simon , Hohberg Martin , Gude Maik , Henning Frank , Kärger Luise
Published: February 2022

The compression molding process of sheet molding compound (SMC) is an economical manufacturing process for lightweight parts. However, molding defects, such as fiber matrix separation, and fiber re-orientation, may develop during the molding process in confined regions, such as ribs and bosses. Hence, the mechanical properties of the composite depend on the local fiber architecture. Consequently, this work compares the predictive capabilities of tensor-based and directly modeled process simulation approaches regarding compression force, fiber volume content and fiber orientation on the example of honeycomb structures molded from SMC. The results are validated by micro-computed tomography and thermal gravimetric analysis. The fiber orientation in the honeycomb varies between individual samples because a sheet molding compound is macroscopically heterogeneous and thus the fiber architecture is strongly influenced by random events. Tensor-based fiber orientation models can not reliably predict fiber volume content and fiber orientation in the part’s thickness direction if there is a lack of scale separation. Therefore, directly modeled process simulations should be preferred in cases in which fiber length and mold dimensions prohibit scale separation. The prediction of fiber volume content is a difficult task and no simulation can predict the severity of fiber matrix separation precisely in all cases.
Micromechanical Analysis of a Bio-Sandwich Application for Cylinder under Pressure
Authors: Habbar Ghania , Maizia Abdelhakim , Hocine Abdelkader , Ribeiro João , Dhaou Mohamed Houcine
Published: February 2022

In recent years, there has been a growing replacement of synthetic fibers by natural ones, particularly by autochthonous materials. In the case of Algeria, the most abundant plant resources are the PALF (Pineapple leaf fiber), the date palm, and the Alfa fibers. In this work, the development and use of analytical and numerical methods are proposed to predict the mechanical properties of layers based on natural fibers that will be applied to manufacture skins of the sandwich cylinder. To achieve these predictions, four analytical models were used, namely the Halpin–Tsai, the Chamis, the Hashin vs. Rosen, and the ROM. The analytical results were compared with the numerical simulations and experimental data. The prediction of the elastic properties of the three fiber-based eco-composites showed an important dispersion in terms of stiffness.
Environmental Stressors and Pathology of Marine Molluscs
Authors: Esposito Giuseppe , Pastorino Paolo , Prearo Marino
Published: February 2022

Mollusca is one of the most species-rich phylum of the Animal Kingdom, comprising a wide range of both terrestrial and aquatic organisms [...]
Performance of a Real Time PCR for Pneumocystis jirovecii Identification in Induced Sputum of AIDS Patients: Differentiation between Pneumonia and Colonization
Authors: Chagas Oscar José , Nagatomo Priscila Paiva , Pereira-Chioccola Vera Lucia , Gava Ricardo , Buccheri Renata , Del Negro Gilda Maria Barbaro , Benard Gil
Published: February 2022

Pneumocystis jirovecii pneumonia (PcP) remains an important cause of morbimortality worldwide and a diagnostic challenge. Conventional methods have low accuracy, hardly discriminating colonization from infection, while some new high-cost or broncho-alveolar lavage-based methods have limited usefulness in developing countries. Quantitative PCR (qPCR) tests may overcome these limitations due to their high accuracy, possibility of automation, and decreasing cost. We evaluated an in-house qPCR targeting the fungus mtSSU gene using induced sputum. Sensitivity of the assay (ten target gene copies/assay) was determined using recombinant plasmids. We prospectively studied 86 AIDS patients with subacute respiratory symptoms in whom PcP was suspected. qPCR results were determined as quantification cycles (Cq) and compared with a qualitative PCR performed in the same IS, serum 1,3-β-D-Glucan assay, and a clinical/laboratory/radiology index for PcP. The qPCR clustered the patients in three groups: 32 with Cq ≤ 31 (qPCR+), 45 with Cq ≥ 33 (qPCR-), and nine with Cq between 31-33 (intermediary), which, combined with the other three analyses, enabled us to classify the groups as having PcP, not P. jirovecii-infected, and P. jirovecii-colonized, respectively. This molecular assay may contribute to improve PcP management, avoiding unnecessary treatments, and our knowledge of the natural history of this infection.
Identification of Independent Risk Factors for Skin Complications in a Multifactorial Logistic Regression Analysis of Simultaneous Immediate Autologous Breast Reconstruction and Skin Reduction Mastectomy in Large and Ptotic Breasts Using an Inferiorly Based Deepithelialized Dermal Breast Flap
Authors: Vollbach Felix H. , Thomas Benjamin F. , Fansa Hisham
Published: February 2022

Autologous immediate breast reconstruction in large and ptotic breasts remains challenging. We aimed to identify independent risk factors for impaired wound healing and nipple necrosis after skin reducing wise pattern mastectomy in autologous reconstruction with an auxiliary deepithelialized inferiorly based dermal flap (IBDF). Methods. This retrospective study examined patients with wise pattern mastectomy with autologous immediate breast reconstruction (IBR) between 2017 and 2019. All cases of large and ptotic breasts were included. Demographic, oncologic, reconstructive, and surgical data were compiled, and multifactorial binary logistic regression models identified independent predictors for skin complications and nipple areolar complex (NAC) necrosis. Results. Of 591 autologous breast reconstructions, 62 (11%) met the inclusion criteria. Overall wound complication rate was 32% (n = 20, DIEP 11, thigh 9, p = 0.99), including 26% minor (n = 16, non-surgically treated) and 7% major complications (n = 4, surgically treated). Complete NAC necrosis occurred in one case. Nipple sparing mastectomy (NSM) (p = 0.003), high BMI (p = 0.019), longer operation time (p = 0.044) and higher patient age (p = 0.045) were independent risk factors for skin complications. Using internal mammary artery perforators (IMAP) as recipient vessels did not result in increased complication rates (p = 0.59). Conclusion. Higher patient age, BMI, and operation time (OT) significantly increase the risk for skin complications in combined reduction wise pattern mastectomies with autologous IBR. In this context, IBDFs help preserve the inframammary fold, providing vasculature to the T-junction and the mastectomy skin flaps. Acceptable complication rates can be achieved in large and ptotic breasts, regardless of preoperative chemotherapy or radiation. Gentle tissue handling with minimal thermal trauma preserves internal mammary artery perforators (IMAPs) as recipient vessels. In cases of flap failure and alloplastic conversion, the IBDF can serve as an autoderm, protecting the implant from exposure
Patient and Physician Preferences for Regimen Attributes for the Treatment of HIV in the United States and Canada
Authors: Gelhorn Heather , Garris Cindy , Arthurs Erin , Spinelli Frank , Cutts Katelyn , Chua Gin Nie , Collacott Hannah , Lebouché Bertrand , Lowman Erik , Rice Howard , Heidenreich Sebastian
Published: February 2022

A long-acting injectable (LAI) antiretroviral therapy (ART) regimen is now available as a treatment option for virologically suppressed adults with HIV-1. This study assessed preference for a LAI regimen using an online survey of virally suppressed people living with HIV (PLWH) and physicians treating HIV in the US and Canada. Preference was elicited in a discrete choice experiment (DCE) with three choice options (switch to a LAI regimen, switch to another daily oral ART regimen, or stay on their current daily oral ART regimen) and four treatment attributes. A total of 553 PLWH and 450 physicians completed the survey. From the DCE results, 59% of PLWH were predicted to prefer a LAI over an alternative oral ART or staying on their current oral treatment, and 55–66% of physicians were predicted to recommend LAI for PLWH, depending on the treatment challenge scenario presented. PLWH indicated LAI would remove daily reminders of HIV (75%) and reduce feelings of being stigmatized (68%). A majority of PLWH and physicians preferred a LAI over oral ART to overcome treatment challenges such as daily pill burden and adherence. These benefits of LAI ART along with preferences of PLWH and physicians can help to inform ART choice.
Diagnostic Accuracy of the Deep Learning Model for the Detection of ST Elevation Myocardial Infarction on Electrocardiogram
Authors: Choi Hyun Young , Kim Wonhee , Kang Gu Hyun , Jang Yong Soo , Lee Yoonje , Kim Jae Guk , Lee Namho , Shin Dong Geum , Bae Woong , Song Youngjae
Published: February 2022

We aimed to measure the diagnostic accuracy of the deep learning model (DLM) for ST-elevation myocardial infarction (STEMI) on a 12-lead electrocardiogram (ECG) according to culprit artery sorts. From January 2017 to December 2019, we recruited patients with STEMI who received more than one stent insertion for culprit artery occlusion. The DLM was trained with STEMI and normal sinus rhythm ECG for external validation. The primary outcome was the diagnostic accuracy of DLM for STEMI according to the three different culprit arteries. The outcomes were measured using the area under the receiver operating characteristic curve (AUROC), sensitivity (SEN), and specificity (SPE) using the Youden index. A total of 60,157 ECGs were obtained. These included 117 STEMI-ECGs and 60,040 normal sinus rhythm ECGs. When using DLM, the AUROC for overall STEMI was 0.998 (0.996–0.999) with SEN 97.4% (95.7–100) and SPE 99.2% (98.1–99.4). There were no significant differences in diagnostic accuracy within the three culprit arteries. The baseline wanders in false positive cases (83.7%, 345/412) significantly interfered with the accurate interpretation of ST elevation on an ECG. DLM showed high diagnostic accuracy for STEMI detection, regardless of the type of culprit artery. The baseline wanders of the ECGs could affect the misinterpretation of DLM.
Special Issue: “Gastrointestinal Cancers and Personalized Medicine”
Authors: Nobili Stefania , Mini Enrico
Published: February 2022

Gastrointestinal cancers represent more than 25% of all diagnosed cancers and more than 36% of cancer-related deaths worldwide [...]
Periodontitis, Metabolic and Gastrointestinal Tract Diseases: Current Perspectives on Possible Pathogenic Connections
Authors: Gheorghe Dorin Nicolae , Camen Adrian , Popescu Dora Maria , Sincar Cerasella , Pitru Allma , Ionele Claudiu Marinel , Nicolae Flavia Mirela , Danilescu Claudia Monica , Roman Alexandra , Florescu Cristina
Published: February 2022

Comprehensive research conducted over the past decades has shown that there is a definite connection between periodontal and systemic conditions, leading to the development and consolidation of the “periodontal medicine” concept. The 2018 classification of periodontal conditions uses this concept as a key element of the precise diagnosis of and individualized therapeutical protocols for periodontitis patients. The topic of this review is the pathogenic connections that exist between periodontal disease and metabolic/digestive tract conditions. It is important to remember that the oral cavity is a key element of the digestive tract and that any conditions affecting its integrity and function (such as periodontitis or oral cancer) can have a significant impact on the metabolic and gastrointestinal status of a patient. Thus, significant diseases with links to metabolic or digestive disruptions were chosen for inclusion in the review, such as diabetes mellitus, hepatic conditions and gastric cancers. Periodontal pathogenic mechanisms share several significant elements with these conditions, including mutual pro-inflammatory mediators, bacterial elements and genetic predisposition. Consequently, periodontal screening should be recommended for affected patients, and conversely, periodontitis patients should be considered for careful monitoring of their metabolic and digestive status.
Effectiveness of Platelet-Rich Plasma Therapy in Androgenic Alopecia—A Meta-Analysis
Authors: Georgescu Simona Roxana , Amuzescu Andreea , Mitran Cristina Iulia , Mitran Madalina Irina , Matei Clara , Constantin Carolina , Tampa Mircea , Neagu Monica
Published: February 2022

Platelet-rich plasma (PRP) represents a novel therapy tested and is used more and more frequently in dermatology and cosmetic surgery for a variety of conditions, including androgenic alopecia (AGA), a common condition with a complex pathogenesis involving genetic factors, hormonal status and inflammation. We performed an extensive literature search which retrieved 15 clinical trials concerning the use in AGA of PRP therapy, alone or in combination, in male, female or mixed patient groups. A quantitative statistical meta-analysis of n = 17 trial groups proved significant increases in hair density from 141.9 ± 108.2 to 177.5 ± 129.7 hairs/cm2 (mean ± SD) following PRP (p = 0.0004). To the best of our knowledge, this is the first meta-analysis that proved a statistically significant correlation between the number of PRP treatments per month and the percentage change in hair density (r = 0.5, p = 0.03), as well as a negative correlation between the mean age of treatment group and the percentage change in hair density (r = −0.56, p = 0.016). Other factors considered for analysis were the PRP preparation method, amount used per treatment, hair diameter, terminal hairs and pull test. We conclude that PRP represents a valuable and effective therapy for AGA in both males and females if patients are rigorously selected.
Dynamics of Soluble Factors and Double-Negative T Cells Associated with Response to Renal Denervation in Resistant Hypertension Patients
Authors: Delgado-Silva Joana , Rodrigues-Santos Paulo , Almeida Jani-Sofia , Santos-Rosa Manuel , Gonçalves Lino
Published: February 2022

The role of the immune system, and hence inflammation, in the pathophysiology of hypertensive patients is not clear. Until now, most clinical and biochemical parameters have failed to predict a positive response to renal denervation (RDN). Our aim was to evaluate the immune response in a cohort of patients treated by RDN, through the analysis of cytokine, chemokine, and growth factor behavior. A population of 21 resistant hypertension patients, treated by RDN, was evaluated at six months and one year. Response was defined as a drop of ≥5 mmHg in ambulatory blood pressure monitoring. Sixty-seven percent and 81% of patients clinically responded after six months and one year, respectively. There were no complications or safety issues. Plasmatic levels of 45 cytokine, chemokine, and growth factors were quantified at four different times, pre- and post-procedure. Baseline characteristics were similar between groups, except that active smoking was more frequent in non-responders at one year. Regulated on activation, normal T cell expressed, and secreted (RANTES/CCL5) levels were significantly lower in responders, both at baseline and at 30 days (p = 0.037), and a level ≤15,496 pg/mL was the optimal cutoff, for prediction of a response. IL-15, IL-17A, IL-27, and leukemia inhibitory factor varied significantly in time, with an acute rise being observed 24 h after RDN. Our group has previously showed that HLA-DR+ double-negative (DN) T cells were significantly lower in responders. There was a positive correlation between IL-13, -27, and -4, and DN T cells, and a negative correlation between the latter and SDF-1α and TNF-α, at baseline. Low plasmatic levels of the chemokine RANTES/CCL5 was the most significant result associated with RDN response and may help to identify the best candidates among patients with true resistant hypertension. Pro-inflammatory cytokines correlated negatively with DN T cells in responders, a finding compatible with an enhanced inflammatory milieu present in this extremely high cardiovascular risk cohort.
Sex-Specific Differences in Pre-Stroke Characteristics Reveal Vulnerability of Elderly Women
Authors: Hoyer Carolin , Schlenker Jan , Sandikci Vesile , Ebert Anne , Wittayer Matthias , Platten Michael , Szabo Kristina
Published: February 2022

While the sexually dimorphic character of ischemic stroke has been acknowledged along several dimensions, age-specific sex disparities regarding pre-stroke characteristics in particular have received comparatively little attention. This study aimed to identify age-dependent associations between sex and risk factors, premorbidity, and living situation in patients with ischemic stroke to foster the continuing development of dedicated preventative strategies. In a retrospective single-center study, data of patients with acute ischemic stroke (AIS) admitted to the Department of Neurology, University Hospital Mannheim, Germany, between June 2004–June 2020 were included; AIS frequency, vascular risk factors, premorbidity, living situation, and stroke etiology were analyzed across sexes and different age spectra. From a total of 11,003 patients included in the study, 44.1% were female. Women aged >70–≤90 years showed a pronounced increase in stroke frequency, lived alone significantly more frequently, and had a significantly higher degree of pre-stroke disability than men; however, only hypertension and atrial fibrillation were more prevalent in women in this age segment. The seventh and eighth decades are a critical time in which the pre-stroke risk profile changes resulting in an increase in stroke morbidity in women. This emphasizes the relevance of and need for an approach to stroke prevention that is both targeted and integrative.
Urban Scene Protection and Unconventional Practices—Contemporary Landscapes in World Heritage Cities of Spain
Authors: García-Esparza Juan A.
Published: February 2022

This paper theoretically examines the extent to which visual perception has excluded alternative forms of culture in World Heritage cities and towns in Spain. To do so, an initial review is carried out of the UNESCO framework and criteria which establishes the outstanding universal value of Spanish cities and towns. This is followed by a review of minor concepts such as informality and creativity, which are related to alternative conceptions of culture and heritage. Thirdly, a review of the literature provides an overview of the appraisal of the social value in the heritage realm. This is achieved through the Historic Urban Landscape approach, which is adapted to specific forms of local appraisal. An analysis of policy helps explain the need to review principles of protection in terms of ethical evaluations. The analysis shows that outdated policies clash with contemporary assessment and participatory methods of heritage-making.
Analysis of Effects of Recent Changes in Hydrothermal Conditions on Vegetation in Central Asia
Authors: Xing Xiuwei , Qian Jing , Chen Xi , Chen Chaoliang , Sun Jiayu , Wei Shujie , Yimamaidi Duman , Zhanar Zhahan
Published: February 2022

Understanding the relationship of hydrothermal conditions to vegetation changes is conducive to revealing the feedback mechanism connecting climate variations and vegetation. Based on the methods of Theil–Sen median analysis, and the Mann–Kendall trend test, this research investigated the spatiotemporal vegetation dynamics in Central Asia using the Normalized Difference Vegetation Index (NDVI) and grid climate data from 1982 to 2015. Further, the contributions of hydrothermal conditions to vegetation changes were quantified using a boosted regression tree model (BRT). The results demonstrated that the spatiotemporal characteristics of vegetation dynamics exhibited significant differences in different seasons, and most pixels showed increasing trends in the growing season and spring. Boosted regression tree analysis indicated that the contributions of hydrothermal conditions to vegetation dynamics exhibited temporal and spatial heterogeneity. During the annual, growing season, and summer examination periods, the contribution value of the increase in warming conditions (temperature or potential evapotranspiration) to vegetation degradation in the region due to the hydrothermal tradeoff effect (water) was 49.92%, 44.10%, and 44.95%, respectively. Moreover, the increase in warming conditions promoted vegetation growth, with a contribution value of 59.73% in spring. The contribution value of the increase in wetting conditions (precipitation or soil moisture) to vegetation growth was 48.46% in northern Central Asia, but the contribution value of the increase in warming conditions to vegetation degradation was 59.49% in Ustyurt Upland and the Aral Sea basin in autumn. However, the increase in warming conditions facilitated irrigation vegetation growth, with a contribution value of 59.86% in winter. The increasing potential evapotranspiration was the main factor affecting vegetation degradation in the Kyzylkum Desert and Karakum Desert during the annual, growing season, and autumn examination periods. Precipitation and soil moisture played decisive roles in vegetation dynamics in northern Central Asia during the growing season, summer, and autumn. This research provides reference information for ecological restoration in Central Asia.
Impact on Soil Physical Properties Related to a High Mechanization Level in the Row Thinning of a Korean Pine Stand
Authors: Baek Kigwang , Lee Eunjai , Choi Hyungtae , Cho Minjae , Choi Yunsung , Han Sangkyun
Published: February 2022

In ground-based harvesting, machine traffic can cause soil physical disturbances including excessive compaction, the displacement of the soil surface and topsoil, and rutting. These impacts can indirectly hamper seedling survival and tree growth because of reduced soil permeability and root growth. However, the extent of soil disturbance in mechanized row thinning by harvester and forwarder systems in South Korea is unclear. Therefore, our objectives were to determine the impacts of two types of harvesters, soil textures, and extraction methods on soil compaction and rutting in a Korean Pine stand. The results showed that the machine passes affected soil disturbances. The soil bulk density inside the tracks (at 0–10 and 10–20 cm soil depths) after harvester passes (wheeled vs. tracked) increased by 17 and 27% and 30 and 20%, respectively. The largest impact was recorded for the forwarding track and significant differences were observed between the track and reference locations. Furthermore, the rutting depth in the forwarding trails was significantly higher than in the harvester trails. Thus, the application of a brush mat on harvester and forwarder trails may reduce machine-induced soil compaction. These results provide useful information to help forest engineers and field managers design environmentally sound ground-based harvesting operations.
Checked Syllables, Checked Tones, and Tone Sandhi in Xiapu Min
Authors: Chai Yuan , Ye Shihong
Published: February 2022

A “checked” syllable usually refers to one with a short vowel and an oral or glottal coda, which results impressionistically in a “short” and “abrupt” quality. Although common in languages of the world, it is unclear how to characterize checked syllables phonetically. In this study, we investigated the acoustic features of checked syllables in citation and sandhi forms in Xiapu Min, an under-documented language from China. We conducted a production experiment and analyzed the F0, phonatory quality, vowel duration, and vowel quality in checked syllables. The results show that, in citation tones, checked syllables are realized with distinct F0 contours from unchecked syllables, along with glottalization in the end and a shorter duration overall. In sandhi tones, checked syllables lose their distinct F0 contours and become less glottalized. However, the shorter duration of checked syllables is retained in sandhi forms. This study lays out the acoustic properties that tend to be associated with checked syllables and can be used when testing checked syllables in other language varieties. The fact that in Xiapu Min sandhi checked tones become less glottalized but preserve their shorter duration suggests that, when checked syllables become unchecked diachronically, glottalization might be lost prior to duration lengthening.
Metal versus Fiberglass Post-Orthodontic Retainers Short-Term Effects on Plaque Index and Microbial Colonization: An Observational Study
Authors: Mummolo Stefano , Quinzi Vincenzo , Nota Alessandro , Marino Carla , Pittari Laura , Manenti Rebecca Jewel , Tecco Simona
Published: February 2022

In orthodontics, post-treatment retention phase is crucial for maintaining the obtained clinical results. In cases of crowding, a bonded fixed retainer is often chosen to maintain teeth alignment in the anterior sector of the lower dental arch. A fixed retainer can remain in the mouth for years. Therefore, it is important that it is applied with harmless materials for the level of plaque control. The present study aimed to investigate the salivary concentrations of Streptococcus mutans (S. mutans) and Lactobacilli, and the Sillness and Loe plaque index, in patients wearing metal wire versus fiberglass orthodontic retainers. Forty post-orthodontic patients were included in the sample: in 20 subjects a metal wire retainer was applied (MR), while in the others a fiberglass retainer was applied (FR). The variables were recorded at baseline (T0), after 1 month (T1), and after 2 months (T2) of follow-up. The percentage of patients with a level of S. mutans and Lactobacilli colonization > 105 increased over time in the FR group (T0 = 0%, T1 = 5%, T2 = 35%), compared with the MR group. PI increased in the FR group (T0 = 0, T1 = 14, T2 = 27), and remained almost the same in the MR group (T0 = 3, T1 = 0, T2 = 2). From the present results it appears that the metal wire retainer is better than the fiberglass retainer for the level of plaque control performed by the patients.
High Dose Intravenous Vitamin C as Adjunctive Therapy for COVID-19 Patients with Cancer: Two Cases
Authors: Guo Guangling , Chen Qi , Luo Guoshi , Meng Zhongji , Lei Pan , Chen Ping , Drisko Jeanne A.
Published: February 2022

Background: Related to the SARS-CoV-2 pandemic leading to COVID-19 illness, patients with cancer comorbidity are known to have a higher risk of developing severe viral-related events, including death. To date, there are few treatments with proven efficacy for COVID-19. Vitamin C administered intravenously (IVC) has been extensively investigated in cancer treatment with a known safety profile and has been proposed to play a role in managing COVID-19. IVC was used to treat COVID-19 patients in hospitals in China, USA, and Europe with reported benefits. We report here unexpected beneficial results from the use of IVC in two severely ill oncology patients with documented COVID-19 lung disease. Case Report: two oncology patients were diagnosed with SARS-CoV-2 infection. Prior to receiving IVC, lung infiltrates and systemic inflammation in both patients were progressing despite multiple anti-viral, antibiotic, and anti-inflammatory treatments with intensive supportive care. Both patients subsequently received 12 g of IVC delivered intravenously over 30 min, given 2 times daily for 7 days. Serial SARS-CoV-2 nucleic acid tests showed that the viral load was negative only after the 7-day IVC treatment. In both patients after receiving IVC infusions, imaging by chest CT or X-ray showed improving lung infiltrates. There were reductions in systematic inflammation by high-sensitivity C-reactive protein (hsCRP), and Interleukin-6 (IL-6) testing. No adverse events were observed related to IVC treatment. Conclusion: the use of high-dose IVC demonstrated unexpected clinical benefits in treating COVID-19 in two cancer patients presenting with complicated severe comorbidities where an unfavorable prognosis was anticipated.
Current Approaches in Molecular Enzymology
Authors: Szabo Eszter , Ambrus Attila
Published: February 2022

Enzymes are the main executioners of living organisms [...]
Ethyl P-Methoxycinnamate: An Active Anti-Metastasis Agent and Chemosensitizer Targeting NFκB from Kaempferia galanga for Melanoma Cells
Authors: Lallo Subehan , Hardianti Besse , Sartini Sartini , Ismail Ismail , Laela Dewi , Hayakawa Yoshihiro
Published: February 2022

The most common type of skin cancer is melanoma. While significant advances in chemotherapy have occurred in a few instances, only marginal progress has been made in treating metastatic melanoma. Natural medicine has traditionally been used to treat various illnesses, including cancer. The purpose of this study was to identify the active compound in Kaempferia galanga, which could be used to treat melanoma as an anti-metastasis and chemosensitizer agent. The active compound in K. galanga was isolated and identified using chromatography and spectroscopy techniques, and given six compounds. Inhibitory activity on NFκB activation and cell viability was determined using reporter assay methods. Among the isolated compounds, ethyl p-methoxycinnamate (EPMC) demonstrated potent NFκB inhibitory activity against melanoma cell B16F10- NFκB Luc2 with an IC50 of 88.7 μM. Further investigation was conducted by evaluating the anti-metastasis effect of EPMC in vitro by using wound-healing assays, invasion tests, and molecular mechanism assays using Western blotting. NFκB has been implicated in tumorigenesis through the PI3K/Akt/NFκB pathway. The results of this study indicated that EPMCs act as inhibitors of p38 and thereby Akt phosphorylation inhibitors at serine 473, inhibiting NFκB-dependent transcription. Further analysis with paclitaxel demonstrated that the combinations could sensitize to apoptosis in response to well-known chemotherapy agents. Additional studies were conducted using the human melanoma cancer cell line SK-Mel 28. Along with the induction of apoptosis, we observed an increase in p-γH2AX expression (a molecular marker for double strand breaks in DNA damage) in response to treatment with paclitaxel and EPMC. The result showed EPMC to be a potential, viable adjuvant for improving the clinical efficacy of anti-metastatic and cancer chemotherapy.
Theoretical and Numerical Investigations on Static Characteristics of Aerostatic Porous Journal Bearings
Authors: Gu Yandong , Cheng Jinwu , Xie Chaojie , Li Longyu , Zheng Changgeng
Published: February 2022

To investigate the static characteristics of aerostatic journal bearings with porous bushing, the flow model—in which the compressibility of lubricating gas is considered—is established based on the Reynolds lubrication equation, Darcy equation for porous material, and continuity equation. With the finite difference method, difference schemes for non-uniform grids, relaxation method, and virtual node method, the numerical method for the governing equations of compressible flow in porous journal bearings is proposed. The effects of nominal clearance of bearings and compressibility of gas on the static characteristics are analyzed. Under the same minimum film thickness and the same gas compressibility, as the nominal clearance widens, the load capacity, mass flow rate, and power consumption increase. Under the same minimum film thickness and the same nominal clearance, with the increase in gas polytropic index, the load capacity strengthens, while the mass flow rate and power consumption decline. This study could provide a reference for the design of porous journal bearings.
A Mixed-Integer Program for Drawing Orthogonal Hyperedges in a Hierarchical Hypergraph
Authors: Fridman Gregory , Vasiliev Yuri , Puhkalo Vlada , Ryzhov Vladimir
Published: February 2022

This paper presents a new formulation and solution of a mixed-integer program for the hierarchical orthogonal hypergraph drawing problem, and the number of hyperedge crossings is minimized. The novel feature of the model is in combining several stages of the Sugiyama framework for graph drawing: vertex ordering, the assignment of vertices’ x-coordinates, and orthogonal hyperedge routing. The hyperedges of a hypergraph are assumed to be multi-source and multi-target, and vertices are depicted as rectangles with ports on their top and bottom sides. Such hypergraphs are used in data-flow diagrams and in a scheme of cooperation. The numerical results demonstrate the correctness and effectiveness of the proposed approach compared to mathematical heuristics. For instance, the proposed exact approach yields a 67.3% reduction of the number of crossings compared to that obtained by using a mathematical heuristic for a dataset of non-planar graphs.
Study on Flow Distribution and Structure Optimization in a Mix Chamber and Diffuser of a CO2 Two-Phase Ejector
Authors: Zheng Lixing , Hu Hongwei , Wang Weibo , Zhang Yiyan , Wang Lingmei
Published: February 2022

This paper establishes a mathematic model of a CO2 two-phase ejector to investigate flow distribution in the components of a mixing chamber and diffuser. The suction chamber was modeled using the characteristic line method to describe the development process of the supersonic expansion wave, and the mixing chamber, as well as diffuser models, were built based on the double-flow model. The reliability of the model was verified by experimental data. The distributions of flow parameters along the axis of the mixing chamber and diffuser were analyzed under different expansion ratios of the ejector. Structure optimizations of the mixing chamber and diffuser were conducted. The results showed that the primary flow temperature gradually increased along the axis of the mixing chamber and diffuser, but the Mach number distribution decreased for a certain ejector expansion ratio. The temperature and Mach number of the secondary flow showed the opposite trend. Moreover, at the initial stage of mixing, the fluid pressure increased rapidly, and the Mach number of the primary flow decreased rapidly. The gas-phase fraction of primary flow increased gradually in the mixing chamber and was stable in the diffuser. When the length–diameter ratio of the mixing chamber was about 10.8–12, it was beneficial to mix uniformity, and when the expansion angle of the diffuser was 4–6°, the ejector had a better ejector efficiency.
Optimal Operation for Reduced Energy Consumption of an Air Conditioning System Using Neural Inverse Optimal Control
Authors: Muñoz Flavio , Garcia-Hernandez Ramon , Ruelas Jose , Palomares-Ruiz Juan E. , Álvarez-Macías Carlos
Published: February 2022

For a comfortable thermal environment, the main parameters are indoor air humidity and temperature. These parameters are strongly coupled, causing the need to search for multivariable control alternatives that allow efficient results. Therefore, in order to control both the indoor air humidity and temperature for direct expansion (DX) air conditioning (A/C) systems, different controllers have been designed. In this paper, a discrete-time neural inverse optimal control scheme for trajectories tracking and reduced energy consumption of a DX A/C system is presented. The dynamic model of the plant is approximated by a recurrent high-order neural network (RHONN) identifier. Using this model, a discrete-time neural inverse optimal controller is designed. Unscented Kalman filter (UKF) is used online for the neural network learning. Via simulation the scheme is tested. The proposed approach effectiveness is illustrated with the obtained results and the control proposal performance against disturbances is validated.
Fractional Calculus and Time-Fractional Differential Equations: Revisit and Construction of a Theory
Authors: Yamamoto Masahiro
Published: February 2022

For fractional derivatives and time-fractional differential equations, we construct a framework on the basis of operator theory in fractional Sobolev spaces. Our framework provides a feasible extension of the classical Caputo and the Riemann–Liouville derivatives within Sobolev spaces of fractional orders, including negative ones. Our approach enables a unified treatment for fractional calculus and time-fractional differential equations. We formulate initial value problems for fractional ordinary differential equations and initial boundary value problems for fractional partial differential equations to prove well-posedness and other properties.