bluuebunny/crossref_metadata_2025 huggingface dataset:
Pounding of seismically designed low-rise reinforced concrete frames
Authors: Hansapinyo Chayanon , Wongmatar Piyapong , Vimonsatit Vanissorn , Chen Wensu
Published: November 2018

Substantial damage to buildings from seismic pounding is the result of earthquakes in many urban areas. This study investigated the effects of pounding in low-rise buildings, which had been individually designed for seismic resistance, using a three-dimensional numerical model. The pounding between the heavier and lighter buildings was conducted for four cases with floor to floor collision and zero separation gap; the total heights of the buildings were varied. The ratio of the storey mass between the heavier to the lighter buildings in all cases was 1·7. The results demonstrated that the heavier buildings were almost unaffected by the collision, and that seismic design without consideration of pounding is acceptable. However, the pounding had more influence on lighter buildings. A significant increase of inter-storey drift and the storey shear force was found. At the top floor of the lighter building, the inter-storey drift and the storey shear force were increased in the range of 35–73% and 20–46%, respectively, compared with no-pounding events. In addition, severe damage at beam–column joints was found. Hence, lighter buildings require special attention during a seismic pounding event.
Highly Skilled Russian Migrants in London
Authors: Земнухова Лилия Владимировна
Published: November 2018

This article analyses Russian IT professionals as highly skilled migrants in London. The context of Russian-British migration has determined the emergence and development of four waves: each of them represents a certain set of circumstances for newcomers to move to, to work in, and to live in the global city. The main idea is to show the transformation of a specific population through the four waves of highly skilled migration to London. This study is based on biographical interviews and observations collected in London in 2013–2015. I claim that the dynamics of local professional and language-based communities, on one hand, were determined by these waves of highly skilled Russian migration, and on the other, shaped their practices of capitalization of various resources1.

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1 The article is a part of the project, which is fulfilled as a government order work funded from the federal budget of the Russian Federation (“Structurization of social space in the modern city”, No AAAA-A17-117030110145-0).
ChroDrip - IMAC v1
Authors: Frommholz David , Stefanczyk Nadine , Ehl Alexandra
Published: November 2018

Purification Guide for the Isolation of Histidine-tagged Proteins with ChroDrip Columns by DALEX Biotech. ChroSpin-IMAC by DALEX Biotech offers a robust and convenient way to isolate polyhistidine-tagged protein from bacterial, mamalian, and insect cell cultures. Immobilized metal affinity chromatography (IMAC) is based on the interaction of the imidazole ring of histidine with transition metal ions immobilized on a solid support. Recombinant proteins with a 3 - 10 histidine fusion tag bind to these metal ions while unwanted proteins are removed by washing with excess binding buffer. Elution of the target protein is achieved by the addition of imidazole, EDTA or a low pH. Easy and quick small scale fusion protein purification from various sources. Low metal leaching from column. Tolerates reducing reagents (e.g. DTT up to 10 mM). The proprietary resin does not shrink or swell in aqueous buffers. High pressure stability. pH stability short term 2 - 8, long term 3 - 8. Excellent thermal stability up to 15 minutes at 120 °C in aqueous buffers at neutral pH. Can be dried for long term storage (80 °C for > 2 h).
ChroDrip - ProteinG v1
Authors: Ehl Alexandra , Frommholz David , Stefanczyk Nadine
Published: November 2018

Purification Guide for the Isolation of Antibodies with ChroDrip Columns by DALEX Biotech. Easy and quick small scale antibody purification from various sources and species. Each ChroDrip column has a binding capacity of > 15 mg/ml (tested with human polyclonal Ig, binding varies between species and clones). The proprietary resin does not shrink or swell in aqueous buffers. High pressure stability. pH stability short term 2 - 8, long term 3 - 8. Excellent thermal stability up to 15 minutes at 80 °C in aqueous buffers at neutral pH. Can be dried for long term storage (80 °C for > 2 h).
ChroPlate - ProteinA v1
Authors: Ehl Alexandra , Frommholz David , Stefanczyk Nadine
Published: November 2018

Purification Guide for the Isolation of Antibodies with ChroPlate Filtration Plates by DALEX Biotech. Easy and quick high throughput antibody purification from various sources and species. Each well of the ChroPlate has a binding capacity of > 1 mg (tested with human polyclonal Ig, binding varies between species and clones). The proprietary resin does not shrink or swell in aqueous buffers. High pressure stability. pH stability short term 2 - 8, long term 3 - 8. Excellent thermal stability up to 15 minutes at 80 °C in aqueous buffers at neutral pH. Can be dried for long term storage (80 °C for > 2 h).
ChroPlate - IMAC v1
Authors: Ehl Alexandra , Frommholz David , Stefanczyk Nadine
Published: November 2018

Purification Guide for the Isolation of Histidine-tagged Proteins with ChroPlate Filtration Plates by DALEX Biotech. ChroPlate-IMAC by DALEX Biotech offers a robust and convenient way to isolate polyhistidine-tagged proteins from bacterial, mamalian, and insect cell cultures. Immobilized metal affinity chromatography (IMAC) is based on the interaction of the imidazole ring of histidine with transition metal ions immobilized on a solid support. Recombinant proteins with a 3 - 10 histidine fusion tag bind to these metal ions while unwanted proteins are removed by washing with excess binding buffer. Elution of the target protein is achieved by the addition of imidazole, EDTA or a low pH. Easy and quick small scale fusion protein purification from various sources. Low metal leaching from column. Tolerates reducing reagents (e.g. DTT up to 10 mM). The proprietary resin does not shrink or swell in aqueous buffers. High pressure stability. pH stability short term 2 - 8, long term 3 - 8. Excellent thermal stability up to 15 minutes at 120 °C in aqueous buffers at neutral pH. Can be dried for long term storage (80 °C for > 2 h).
Recombinase polymerase amplification assay for detection of Mycobacterium ulcerans DNA v1
Authors: Frimpong Michael , Senanu Ahor Hubert
Published: November 2018

This document describes the standard operating procedure for the application of the real time Mu-RPA assay with Exo probe system. The assay detects M. ulcerans DNA (IS2404) from clinical sample or culture suspension. The assay consists of recombinase polymerase amplification TwistDx Exo kit procedure. The reaction mix must be prepared in an environment free of DNA amplicons. Personal protective clothing (i.e. lab coats, gloves) must be used throughout the process.
Perspectivas de Identidade Profissional de Professores que ensinam Matemática presentes em artigos científicos publicados entre 2006-2016
Authors: De Paula Enio Freire , Cyrino Márcia Cristina de Costa Trindade
Published: November 2018

Este artigo apresenta resultados de uma investigação, cujo objetivo foi mapear, descrever e analisar perspectivas do conceito de identidade profissional (IP) de professores que ensinam matemática (PEM), e noções importantes associadas a esse conceito, presentes em artigos científicos, publicados no período 2006 - 2016, nos quais essa temática se apresentou como foco central de investigação. O corpus constituído reúne 23 artigos inventariados em 10 periódicos: 18 em língua inglesa, 1 em língua espanhola e 4 em língua portuguesa. Quatro desses artigos não tratam diretamente da IP de PEM, mas discutem noções consideradas importantes. Dentre aqueles que discutem a IP de PEM, identificaram-se quatro perspectivas epistemológicas: holística, wengeriana, político-reformista e pedagógica dos estágios. Os resultados revelam que essas perspectivas se inter-relacionam e evidenciam a relevância dos elementos socioculturais e dos aspectos afetivos e emocionais no processo de desenvolvimento da IP, envolvidos na formação (inicial e continuada) de PEM.
Ensino de matemática em tempos fluídos: um estudo de inspiração etnomatemática
Authors: Costi Adriana , Giongo Ieda Maria
Published: November 2018

Este texto tem por objetivo explicitar os jogos de linguagem matemáticos expressos por um grupo de estudantes do quarto ano do Ensino Fundamental ao resolverem um conjunto de tarefas que emergiram de uma prática pedagógica centrada nos processos produtivos de uma indústria frigorífica. Os aportes teóricos estão consonância com os estudos do campo da etnomatemática, em seus entrecruzamentos com as ideias da maturidade de Ludwig Wittgenstein. Metodologicamente, a investigação, qualitativa, fez uso de materiais escritos e produzidos pelos estudantes e filmagens das aulas. Tais materiais foram escrutinados à luz da análise do discurso na perspectiva foucaultiana. Os resultados apontaram para a emergência, no desenvolvimento da prática pedagógica, de conteúdos matemáticos usualmente presentes apenas nos anos posteriores de escolarização. Estes mostram a potência dos referenciais teórico-metodológicos para que se repensem os processos de ensino da Matemática, sobretudo nos Anos Iniciais do Ensino Fundamental.
Ensino de Ciências para a Formação do Pedagogo: Concepções de Alunos em Formação
Authors: Higino Santana Isabel Cristina , Pereira Silveira Andréa
Published: November 2018

O Ensino de Ciências no nível fundamental é uma tarefa estimulante e está em ampla discussão nas pesquisas educacionais e dentro da sala de aula. Nesta investigação de abordagem mista e exploratória o objetivo foi compreender as concepções que os discentes de pedagogia têm acerca do entendimento e da relevância do Ensino de Ciências para e na formação de pedagogos. As concepções de um grupo de 31 discentes regularmente matriculados na turma de 6º semestre do curso de Pedagogia da Universidade Estadual do Ceará, Campus da Faculdade de Educação de Itapipoca (UECE-FACEDI), foram acessadas por meio de um relato escrito espontâneo desses alunos. Os dados coletados foram decodificados e categorizados a partir de seu lançamento no software IRAMUTEQ em conjunto com a análise de conteúdo de Bardin. Os resultados mostraram que o Ensino de Ciência é relacionado com a construção de conhecimentos específicos que possibilitam ensinar Ciências nas séries iniciais da educação básica. Os futuros pedagogos destacam também a necessidade de conhecer questões relacionadas ao meio ambiente, para além das bases teóricas do tema. Foi registado preocupação dos entrevistados em compreender o meio, sem, no entanto, se preocupar com a sua contextualização ou mesmo com a utilização da interdisciplinaridade e da ludicidade, como veículo de transposição do conhecimento adquirido na academia e levado pra dentro da sala de aula. É conclusivo, portanto, através desse processo de análise da mensagem, que  o Ensino de Ciências para e na formação do pedagogo deve ser articulado a questões de formação docente, de construção de conhecimentos específicos, de relações com outras disciplinas e de ludicidade, no propósito de contribuir para a formação de indivíduos autônomos, cidadãos e críticos.
Choice of minimally invasive method of treatment of pancreatic pseudocysts: a single center, retrospective study
Authors: Neledova L A , Mizgirev D V , Duberman B L
Published: November 2018

Aim of the study is to evaluate efficacy of different methods of minimally invasive treatment of pancreatic pseudocysts (PPC). Methods. A single center retrospective study of patients with pancreatic pseudocysts (n = 17): 90 males (76.9 %), 27 females (23.1 %) aged 25 to 72 years. The patients underwent external percutaneous drainage (group 1, n = 96) or internal drainage (group 2, n = 21). The diagnosis of pseudocysts included clinical, laboratory (biochemical and bacteriological) and special investigation methods: radiological, endoscopic, ultrasound examination of hepatobiliary zone, computer tomography. Results. Complications in the early postoperative period were observed in patients from both groups 1 and 2. They were related to inefficacy of cystodigestive anastomosis, which required percutaneous drainage in 2 cases (9.5 %), or to formation of pancreatic fistula. Lethal outcomes were not observed. Readmission to surgical department for removal of the drainage was required in 28 (23.9 %) patients from group 1. Internal drainage is considered more advantageous for PPC decompression compared to external one due to persistence of pancreatic fluid passage through gastrointestinal tract. External drainage is associated with frequent external pancreatic fistulae formation as well as prolonged hospital stay and treatment in an outpatient setting worsening the quality of life, but it is an intervention of choice in somatically severely ill patients, in fast growing cyst, imperfectly formed wall and threatening cyst rupture into abdominal cavity or abscess. These aspects prevent from refusal from external drainage for PPC treatment. Conclusion. When choosing the optimal time and type of surgical intervention in PPC, the surgeon should evaluate localization, sizes, maturation of PC wall and its relation to pancreatic duct, somatic state and patient’s individual features.
Features of antioxidant system in patients with obstructive jaundice of benign and malignant origin in dynamics
Authors: Elmanova N G
Published: November 2018

Aim. Study of the features of changes of antioxidant protection in patients with mechanical jaundice of benign and malignant origin in dynamics. Methods. The author studied the role of antioxidant system in the progression of mechanical jaundice of various origins in 104 patients. Groups of patients with a syndrome of mechanical jaundice of benign (62 patients) and malignant origin (42 patients) were isolated. The material of the study was blood from the ulnar vein, which was taken in the morning on an empty stomach before surgery. In the dynamics (on the 7th day after the operation), 53 patients were examined. To assess the state of antioxidant protection, a spectrophotometric method of investigation was used. Results. A high level of malonic dialdehyde, the product of lipid peroxidation, was determined in all patients regardless of origin. There was also a depression of the enzymatic link of antioxidant protection (a decrease in the activity of superoxide dismutase and catalase). After surgical intervention in patients with mechanical jaundice of benign origin, correction of the level of reduced glutathione was observed. In patients with mechanical jaundice of malignant origin in dynamics, the activity of antioxidant enzymes did not differ significantly from the norm (p1-3 = 0,23; p1-3 = 311). Conclusion. After surgical intervention, partial improvement of the condition of patients with mechanical jaundice of benign origin was observed, and dysfunction of antioxidant protection persisted in patients with mechanical jaundice of malignant origin.
Study of antibiotic prescription pattern in PICU of a tertiary care hospital
Authors: Bamel Vivek Virbhan , Shahani Savita Ramesh
Published: November 2018

Background: Infectious disease is one of the importance causes of mortality and morbidity in India, therefore pattern of use of antibiotics requires separate study as they differ from adult, Therefore the aim of our study is to observe the antibiotic prescription in the tertiary care hospital.Methods: A prospective observational study was carried out in the children admitted in PICU. The study was started after obtaining the approval from Institutional Ethics Committee. Written informed consent was obtained from a parent or legal guardian of participants.Results: There were 50 patients were enrolled. The maximum antibiotics were prescribed from cephalosporin class (42; 40.38%) which included ceftriaxone, cefixime, cefotaxim, cefpodoxime. The majority of cephalosporins were prescribed in miscellaneous cases followed by respiratory tract infections, gastrointestinal tract, Central nervous tract infections and nil in CVS cases, secondly higher number of antibiotics prescribed was metronidazole and from Aminopenicillin class which includes amoxiclav and ampicillin and another beta lactamase which includes ureidopenicilin and carbepenem. The majority of aminopenicillin were prescribed in miscellaneous group infections followed by respiratory tract infections.Conclusions: From this study, it can conclude that commonly antibiotic were from 3rd generation cephalosporins followed by metronidazole and other β lactamase inhibitor and all of the antibiotics were prescribed as per the national guidelines.
Comparative evaluation of various analgesic drugs in Wistar rats used as a teaching tool to teach animal experiments to post graduate MD Pharmacology students
Authors: Beg Mirza Atif , Dutta Shakti Bala , Kumar Hitender , Singh Amandeep , Bawa Shalu
Published: November 2018

Background: Postgraduates when join Pharmacology department, they don’t have much idea about guidelines and methods to do animal experiments. This study was designed to sensitize postgraduate students to animal experiments by evaluating the anti-inflammatory effects and the efficacy of diclofenac, tramadol and etoricoxib in reducing paw edema in wistar rats.Methods: The wistar rats of 225-250gram were divided into four groups (n=10 each). In all the groups the paw edema on right paw was created using 0.1ml of 1% formalin subcutaneously. Group 1 rats (control), group 2 were given diclofenac, group 3 were given tramadol and group 4 were given etoricoxib orally. The parameters assessed were water displacement in plethysmography after 2, 4, 24, 48 hours of giving the drug. The circumference of edematous paws after 4 hours, licking of paw was observed over 30minutes of giving the drug and was compared. Pre and post study feedback were obtained from the students by using a preformed questionnaire to assess the knowledge gained by the students.Results: The test showed that all the drugs were effective in reducing inflammation, circumference of edema and the licking duration significantly (p<0.05). The post study answering of questions by postgraduate was 100% in comparison to pre-study answering which was 33%.Conclusions: A short animal study conducted as a teaching tool to postgraduate students was able to teach the guidelines of animal experiments and handling of animals effectively.
An analysis of seriousness, predictability and preventability of adverse drug reactions reported at a tertiary care teaching hospital in Kerala, India: a retrospective observational record based study
Authors: Shajahan Jihana , Parathoduvil Abdul Aslam , Purushothaman Sangeetha
Published: November 2018

Background: Adverse drug reactions (ADR) are the leading cause of mortality and morbidity in all health care systems. Hospital based ADR monitoring and reporting programmes can throw some light upon the profile of ADRs and ways to prevent them, facilitating rational drug use. An attempt has been made in this study to analyse the seriousness, predictability, preventability, severity and outcome of ADRs occurring in a tertiary care hospital.Methods: This was a retrospective observational study based on the data collected from ADRs reported to an approved ADR monitoring centre (AMC). Data collected was evaluated for seriousness, predictability, preventability, severity and outcome using appropriate scales. Simple descriptive statistics was used for analysis.Results: The total number of ADRs reported was 300. Among this 39% reactions were serious. The commonest reason for considering as serious reaction was prolongation of hospitalization. The overall predictability was 40.4%. Total preventability was found to be 18.3%. Assessment of severity showed 55.3 %, 41.7%, 3% reactions in mild, moderate and severe grades respectively. 64.3% patients had recovered from the reaction and 30% were recovering at the time of reporting ADR. Only 0.3% ADRs caused death.Conclusions: Authors hope this study will foster the culture of reporting and analysing ADRs among health care professionals and students. The findings from the study can create awareness among health care professionals regarding the impact of ADRs on the treatment course.
Occurrence of linezolid induced thrombocytopenia and its association with the risk factors: a review article
Authors: A. Radha , H. V. Anuradha , K. Radhika
Published: November 2018

Linezolid is the oxazolidinone group of antibiotic with wide range of activity against the gram positive bacteria including methicillin resistant staphylococcus aureus and penicillin resistant pneumococci and vancomycin resistant enterococci. Patients who are on linezolid were reported to have reversible myelosuppression especially thrombocytopenia and anaemia. Since there are less number of studies regarding the occurrence of thrombocytopenia and the risk factors associated with it, this study was undertaken to evaluate the occurrence of linezolid induced thrombocytopenia and its association with risk factors. It was a systematic review with synthesis of available literature in English language. Articles were retrieved using search terms included “linezolid”, “and”, “or”, “thrombocytopenia” from Clinical key and PubMed, published during 2000 - 2017. Out of 16 studies retrieved, only 7 studies were analysed based on inclusion and exclusion criteria; of them, 3 were found to be prospective and retrospective cohort each and only one was retrospective cross-sectional study. The occurrence of linezolid induced thrombocytopenia range from 18-50% with normal renal function and 57% of incidence associated with renal insufficiency patients. The risk factors were found to be dose of linezolid >18-27mg/kg, body weight of subjects <55kg, creatinine clearance <88.39 to 60ml/min/1.73m2 and baseline platelet count <200*103/mm3, serum albumin concentration, serum creatinine, concomitant caspofungin therapy and duration of linezolid therapy.
Feto-maternal outcome in second versus first stage caesarean delivery in a tertiary medical care centre
Authors: Gupta Nupur , Gupta Taru , Singh Ritu
Published: November 2018

Background: The aim is to study neonatal and maternal outcomes of the caesarean sections performed in first stage versus second stage of labour. Methods: The retrospective analysis of data were done of caesarean section done at Department of Obstetrics and Gynaecology at ESI PGIMSR Basaidarapur New Delhi between January 2016 to December 2016. 45 women, who underwent second stage caesarean section were studied. For each case, two consecutive cases, who underwent caesarean delivery during the first stage of labour were taken as control for the study. Primary maternal outcomes of interest were uterine atonia, transfusion requirement, urinary system injury and postoperative complications. Results: Out of 4477 deliveries, 1466 had caesarean section with a rate of 32%. The rate of second stage caesarean section was 3% of total caesarean section and 1% of total deliveries. Second stage caesarean section had higher maternal and perinatal morbidity like atonic PPH (33.3%), lower uterine segment extension (7%), febrile morbidity (10%), and need for blood transfusion (15%). There were 15.5% NICU admission in second stage caesarean group while none in first stage group. Conclusions: Caesarean section in the second stage of the labour is associated with increased maternal and neonatal morbidities. Special attention is required to the patients undergoing caesarean section in the second stage of the labour. They should be handled by senior and experienced obstetrician. Neonatologist should be present for every second stage caesarean section.
Effect of socio demographic and personal factors on infertility and its association with mental health and social support in North Indian population
Authors: Singh Neetu , Singh Jyotsna
Published: November 2018

Background: Motherhood is a bliss in women’s lives and thus infertility is considered as a social stigma. In present study, we measure the effect of socio-demographic and personal factors on infertility as well as the status of social support and mental health in infertile women.Methods: This was a cross-sectional study comprising 90 infertile women and 90 women in the control group, conducted at Ram Prakash Gupta Memorial Mother and Child State Referral Centre of RMLIMS, Lucknow, India. The questionnaires used were Socio-demographic and personal characteristics, General Health Questionnaire (GHQ-12) to assess mental health, Perceived Social Support Questionnaire (PSSQ). To determine the relationship between socio-demographic characteristics, personal characters, mental health, and social support with infertility, Chi-square test was used.Results: Age, education, and occupation had statistically significant (p<0.05) relation with infertility, high BMI, and caffeine intake also had the significant adverse effect on fertility outcomes (p<0.05). Infertility patients had poor mental health status (higher mean GHQ-12 score 20.8±4.2) and poor perceived social support (lower mean PSSQ score 29.7±7.0).Conclusions: In present study, the socio-demographic factors, lifestyle factors, social support and mental health status is associated with fertility outcomes. Their modifications have the potential to improve reproductive performances. A structured programme of education, social support, and counselling by specialist health professionals should be formulated to improve the quality of life as well as fertility outcomes in infertile patients.
HIV reactivity trends in a tertiary care teaching hospital in Himachal Pradesh: a ten-year ICTC based retrospective analysis
Authors: Kaur Isampreet , Thakur Kamlesh , Chauhan Smriti , Sood Anuradha , Sharma Piyush , Singh Harshvardhan , Sharma Pankaj
Published: November 2018

Background: Despite being a low HIV prevalence nation, India has the third largest number of PLHAs in the world. The study aimed to explore the prevalence, pattern of socio-demographic and epidemiological distribution among HIV sero-positive patients in this part of Himachal Pradesh. Objective was to estimate the prevalence of HIV infection among the clients who had attended the ICTC for a period of ten years, i.e. from 2008 to 2017.Methods: A retrospective descriptive analysis of secondary data from the National AIDS control program from the year 2008 through 2017 was done.Results: Overall prevalence of HIV positivity amongst the clients attending the centre was observed to be 2.1%. Out of the total 55610 clients tested for HIV infection, 40.4% were male, 25.4% were female (excluding ANCs) and 34.2% were Ante-natal cases. Overall, seropositivity was higher among males (58%) than females (40%). However, amongst the groups, higher prevalence has been observed to be present in the females (3.3%) over males (3%) and Ante-natal cases (0.12%). Belonging to the female sex [OR 1.99 (95% CI: 1.77-2.24)] and male sex [OR 2.07 (95% CI: 1.84- 2.33)] had higher odds of having HIV sero-positivity than Ante-natal cases [OR 0.04 (95% CI: 0.02-0.05)]. Heterosexual route of transmission was the major route seen in 70.1%. Maximum HIV seropositivity was in the age group of 25 - 34 years (35.4%).Conclusions: The trends over the last 10 years show no steady pattern. Hence, there is a need for scaled up and sustained efforts focused on the males of reproductive age group for the prevention and control of HIV infection.
The impact of BMI on the plasma glucose and lipid status of women with polycystic ovary syndrome
Authors: Green Kinikanwo I. , Amadi Collins
Published: November 2018

Background: BMI status is theorized to impact on the plasma glucose and lipid parameters of women with polycystic ovary syndrome. Hence, this study was instituted to investigate this theory among women with polycystic ovary syndrome in Port Harcourt, Nigeria.Methods: Medical records of 231 women with PCOS visiting a tertiary health center over a consecutive 10-year period were retrospectively acquired and analyzed. The obtained records were age, weight, height, calculated BMI, fasting plasma glucose and lipid profile (total cholesterol, triglycerides, high-density lipoprotein and low-density lipoprotein). The records were analyzed using Shapiro-Wilk, descriptive, chi-square, two-way analysis of variance, and Pearson’s tests. A two-tailed p-value of <0.05 was considered significant.Results: Overweight and obesity was recorded among 33.3% and 45.5% of the study cohorts respectively. The obese cohorts had higher plasma levels and abnormal frequency of fasting glucose (<0.001), total cholesterol (<0.001), triglycerides (<0.001), high-density lipoprotein (<0.001) and low-density lipoprotein (<0.001) status than the normal weight and overweight cohorts. BMI correlated weakly with glucose (r=0.259; p = 0.003), inversely but weakly with high-density lipoprotein (r: -0.373 p<0.001) and weakly with triglycerides (r=0.316; p<0.001) among overweight cohorts. BMI correlated strongly with fasting glucose (r=0.578; p< 0.001), strongly with total cholesterol (r=0.840; p<0.001), moderately but inversely with high-density (r=-0.490; p=0.004), strongly with triglycerides (r=0.753; p<0.001) and strongly with low-density lipoprotein (r=0.759; p<0.001) among the obese cohorts.Conclusions: Abnormal plasma glucose and lipid parameters seem to be prevalent among obese PCOS patients. Therefore, weight reduction should be a therapeutic target during their treatment.
C4d deposition in native kidney disease and its correlation with proteinuria and serum urea/creatinine
Authors: Pandey Sant , Yadav Sucheta , Babu Suresh , Kumar Ashutosh , Singh Bhupendra P. , Wakhlu Anupam , Singhai Atin , Sonkar S. K. , Pooniya Vishal
Published: November 2018

Background: C4d is a well-known biomarker of the complement cascade. It is derived from cleavage of the labile thioester bond of C4b. This cleavage provides C4d a covalent bond which helps C4d to anchor to nearby cells where immune complexes are deposited. Antibodies dissociate naturally because of relatively weak hydrostatic and Van der Waals forces between antigens and antibodies, whereas covalent bond of C4d has a much longer half-life. For this reason, C4d serves as a footprint of complement activation.Methods: This was a retrospective and prospective cross-sectional study, done at our tertiary care hospital.Results: Authors evaluated 50 cases and 10 controls to adjudge the significance of C4d deposits in native renal diseases. Majority of the patients (44%) were in the age group of 10-20 years followed by 20% in the age group of 31-40 years. 62% of study population were male. Majority of patients were diagnosed with FSGS (16%), followed by membranous nephropathy (14%), lupus nephropathy (14%) and IgA nephropathy (12%). There was correlation of intensity expression of glomerular C4d deposits with presenting 24 hours urinary protein level at the time of biopsy (p value=0.027) but no correlation with urea/creatinine.Conclusions: All patients diagnosed with membranous nephropathy, IgA nephropathy and hypertensive nephropathy showed glomerular C4d deposits, and also diagnosed with IgA nephropathy, post infectious glomerulonephritis, lupus nephritis, minimal change disease, acute/chronic tubulointerstitial nephritis, diabetic nephropathy, hypertensive nephropathy showed tubular C4d deposits. All patients diagnosed with diabetic nephropathy and hypertensive nephropathy showed arterial C4d deposits.
Association of triglycerides/high density lipoprotein cholesterol ratio with insulin resistance in polycystic ovary syndrome
Authors: Karli Sreenivasulu , V. S. Kiranmayi , Namburi Rajendra Prasad , Bitla Aparna Rajeshwar Rao , Sachan Alok
Published: November 2018

Background: Insulin resistance (IR) is frequently observed in women with polycystic ovary syndrome (PCOS). Recent studies advocated that triglyceride to high-density lipoprotein cholesterol ratio (TG/HDL-C) can be used as a simple clinical indicator of IR. Hence, the present study was performed to investigate the use of TG/HDL-C and its association with IR in PCOS.Methods: Forty-one patients with PCOS and 40 healthy age matched women were randomly enrolled. Demographic and clinical characteristics were obtained. Insulin resistance was defined by the homeostasis model assessment for insulin resistance (HOMA-IR) and quantitative insulin sensitivity check index (QUICKI).Results: In PCOS group, the insulin, HOMA-IR and TG/HDL-C ratio were significantly higher (p=0.001) than controls while, QUICKI was lower (p=0.001). Insulin, HOMA-IR were positively correlated with TG/HDL-C (ρ=0.303, p=0.006 and ρ=0.312, p=0.005 respectively) while, QUICKI was negatively correlated (ρ=-0.698, p=0.001). In receiver operating characteristic (ROC) analysis, area under the curve (AUC) for model based on QUICKI levels was better 0.898 (95% CI: 0.811-0.955, p=0.001) than HOMA-IR 0.636 (95% CI: 0.522-0.740, p=0.03). A cut-off value 3.23 for TG/HDL-C is proposed from the model based on QUICKI with best combination of sensitivity 83.3% and specificity 86.7%.Conclusions: Results of present study support that TG/HDL-C ratio may be a simple indicator of IR in PCOS patients which helps clinicians to identify IR in small centers, where the assays for insulin measurement are not available.
Clinicopathological profile of malaria patients in an Central African United Nations hospital
Authors: Kohli Kinshuk , Das Amit K.
Published: November 2018

Background: Malaria is one of the most widespread diseases in the world. It is endemic in 91 countries. Each year 300-500 million cases of malaria are confirmed with 1.5-2.7 million deaths. Malaria is a major public health problem in Africa. A large number of united nations troops are deployed in central Africa making them vulnerable to malaria infection. Present study was undertaken to find out the clinical and laboratory findings of malaria cases in a United Nation’s referral hospital in Central Africa.Methods: It is a retrospective study of 150 confirmed and treated cases of malaria for a period of one year in a referral hospital for peace keeping troops and employees of United Nations in Democratic Republic of Congo (DRC). Patients positive by ICT or slide positive were included. Detailed history, examination and lab features of these patients were recorded and analyzed.Results: A total of 150 patients were included in the study. Out of which 145 patients were of pure P. falciparum malaria, 02 patients had only P. vivax, and 03 patients were having mixed malaria. Fever was the main symptoms found in all patients. Headache was the second most predominant symptoms in (70%) patients. Other signs and symptoms were vomiting (31%), myalgia (38%), jaundice (10%), hepatosplenomegaly (15%), pallor (42%) and decreased urine output (8%). Duration of symptoms was between 4 to 15 days. Thrombocytopenia was observed in 43% patients. Jaundice in (10%), transaminitis in (25%). Anaemia was seen in 53% patients.Conclusions: Malaria was found to be the most common cause of admission in our hospital. It is important to suspect malaria in all persons deployed in DRC and presenting with fever and associated headache, jaundice, transaminitis and thrombocytopenia. Early diagnosis and treatment can be lifesaving to prevent complications and mortality.
Programmatic evaluation of mass drug administration for elimination of lymphatic filariasis and reasons for non-compliance in Gulbarga district, Karnataka in 2015
Authors: Naresh Kumar S. J. , Sunil B. N.
Published: November 2018

Background: Mass drug administration (MDA) to eliminate lymphatic filariasis (LF) is conducted once annually in the identified endemic districts of the country. MDA implementation is assessed by appointed independent teams within 2-3 weeks of the activity to suggest mid-term corrections and strengthen the on-going programme. This study was undertaken to assess the process of MDA implementation, drug coverage and compliance and to identify the reasons for non-compliance.Methods: A survey of four clusters comprising of three rural and one urban area was conducted in Gulbarga, during the third week following the MDA campaign in the district. Information pertaining to the MDA beneficiaries of the 124 evaluated households was collected over a period of three days using a structured proforma. The process of MDA implementation was evaluated at the district, taluk and primary health centre (PHC) levels.Results: From the information collected on 673 inmates of the households surveyed, the coverage and compliance rates of 87.5 and 60.9% respectively was found for diethylcarbamazine citrate and albendazole distribution and consumption. The important reasons for non–compliance to swallow the drugs identified were the beneficiaries being out of station during the campaign, was not aware of it, the drug distributer did not visit the beneficiaries house and fear of side effects of the drugs.Conclusions: Efforts to improve the compliance to consumption of the anti-filarial drugs at Gulbarga district is required for elimination of LF.
A cross-sectional study to assess the health hygiene status of school children in urban field practice area, Bangalore Medical College And Research Institute, Bengaluru
Authors: Ambiger Netravati , Ranganath T. S. , Kavitha M. , Padmini D. B.
Published: November 2018

Background: A large fraction of the world’s illness and death is attributable to communicable diseases. This is especially notable in developing countries where acute respiratory and intestinal infections are the primary causes of morbidity and mortality among young children. Objective of the study was to assess the basic hygiene practices among primary school children in urban field practice area, BMC&RI.Methods: A cross sectional study was carried out during September and October 2016 on a total of 125 school children to assess the level of basic hygiene practices in Urban field practice area, BMCRI. A Random sampling technique was used and pre tested and semi structured questionnaire was read out to the participants during face to face interview and sufficient time was given to the subjects to respond, without probing the answer.Results: Among 125 students, 54% were female and 46% were male.53.6% students take bath on alternate days, 88% students wash their feet daily, and 76% students change their cloth daily.75.2% students had h/o head lice and 57.6% students had h/o dental caries.Conclusions: Out of 125, 56.8% of students have excellent hygiene, 42.4% of students have very good hygiene and 0.8% students have good hygiene.
Evaluation of session sites of routine immunization program in Damoh district of Madhya Pradesh
Authors: Panika Ram Kumar , Gupta Amarnath
Published: November 2018

Background: Vaccination is one of the most effective disease prevention strategies and the success of immunization program in the field depends upon the availability of appropriate vaccines, logistics and proper vaccination practice of health workers. This study was undertaken to assess actual vaccines and logistics availability, vaccination practices and safety issues at session sites of Damoh District of Madhya Pradesh.Methods: Cross-sectional observational study was done using a structured questionnaire. Data was collected from 36 session sites of Damoh District of Madhya Pradesh.Results: The availability of AD syringe of 0.1 ml and 0.5 ml was not adequate. Functional hub cutter was available in 30(83.3%) session sites. Zinc tablets were found only at 32 (88.8%) session sites. Due list of beneficiaries was maintained only by 33 (91.6%) ANMs and 26 (72.2%) ASHA workers. BCG was available in 20 (55.5%) and IPV in 27 (75%) sessions. Vaccine storage conditions were not appropriate in 2 sessions sites. Time of reconstitution was written on reconstituted vaccines in 30 (83.3%). Date and time of opening vial was written in 32 (88.8%) session sites. Only in 28 (77.7%) sessions ANM were cutting Syringe with Hub Cutter. Four key messages were being delivered to parents in only 16 (44.4%) sessions and only in 22 (61.1%) sessions ANM was advising the care giver to wait for 30 minutes after vaccination.Conclusions: There was satisfactory organization of immunization session in terms of availability of logistics and cold chain maintenance but unsatisfactory in terms of vaccination practice.
Evaluation of awareness of job responsibilities and incentives of accredited social health activist in the delivery of health care services
Authors: Panda Mayadhar , Nanda Sikata
Published: November 2018

Background: Accredited social health activist (ASHA) workers are the female health volunteers of the community who takes steps to generate awareness and provide information to the community on determinants of health such as nutrition, hygiene practices and basic sanitation, healthy living condition. Our aim was to evaluate the level of awareness of job responsibilities of ASHA workers, to assess about their awareness about the different incentives in the delivery of health care services and to recommend for improvement on the functioning of the ASHA workers.Methods: A cross sectional study was conducted in Khordha district of Odisha from March 2018 to July 2018. 3 blocks were selected purposively i.e. Bhubaneswar under urban block, Bolagarh under rural block and Banapur a tribal block. Working in the 3 selected blocks, 331 ASHA’s participated in the study.Results: 37% were between 34-39 years and 30% were in the age group of 34-39 years in the rural block and about 40% ASHA workers belonged to 25-29 years of age. For full immunization in the 1st year of life per beneficiary an amount of Rs. 100 is given to an ASHA worker. However, 67 (68.4%) in urban block, 79 (63.2%) in rural block and 83 (76.8%) ASHAs in tribal block were aware about this incentive.Conclusions: The role of ASHAs should be well defined and accordingly with supportive supervision, the ASHAs should be monitored to efficiently & effectively play their roles and discharge duties.
Факторы сбалансированного развития сложных экономических систем производственной сферы и сферы услуг в соответствии с концепцией Индустрия 4.0
Authors: Трофимов Олег Владимирович , Фролов Владислав Генрихович , Каминченко Дмитрий Игоревич , Захаров Владимир Яковлевич , Павлова Ангелина Александровна
Published: November 2018

Цифровые технологии оказывают всё более значимое и всестороннее воздействие на развитие современной экономики. В этой связи, устойчивое и сбалансированное развитие сложных экономических систем производственной сферы и сферы услуг в условиях современной экономики трудно представить себе без учета факторов, связанных с влиянием технологий концепции Индустрия 4.0. Авторы предлагают разные типы классификаций факторов. Факторы были распределены по каждому из четырех установленных Г.Б. Клейнером системных секторов экономики, взаимная пропорциональность которых и обуславливает наличие системной сбалансированности. Также факторы распределены на внутренние и внешние (по отношению к предприятию), на «первичные» и «вторичные» (по степени их влияния на устойчивое и сбалансированное развитие и друг на друга). Кроме того, ещё одним критерием для классификации указанных факторов стала функциональная направленность.
Развитие цифровой экономики в Республике Башкортостан
Authors: Мамлеева Эльвира Рашидовна , Сазыкина Марина Юрьевна , Трофимова Наталья Владимировна
Published: November 2018

В статье рассмотрены различные аспекты функционирования цифровой экономики в России. Проанализированы особенности цифровой экономики в Республике Башкортостан, выявлены основные проблемы, препятствующие развитию цифровой экономики в регионе.
Аналитический центр: объектно-онтологический подход
Authors: Анисимов Олег Сергеевич
Published: November 2018

В статье раскрывается объектно-онтологический подход к способам работы в аналитическом центре, компенсирующем ограниченность ситуационных центров. Вводятся основания преимуществ объектных критериев при анализе реальных целостностей социокультурного типа. Указывается на специфические формы мыслетехники в объектно-онтологическом подходе с учетом результатов разработок в семиотике, логике, методологии. Учитываются притязания на высшие результаты аналитики в рамках идеи «прорыва», изложенной Президентов РФ.
Determining ATP concentration in Candida albicans in process
of forming germ tube forms
Authors: Dróżdż Maria , Seget Sebastian , Czuba Zenon
Published: November 2018

Wstęp

Candida albicans (C. albicans) wchodzi w skład naturalnej mikroflory organizmu człowieka. Jednocześnie jest jedną z najczęstszych przyczyn oportunistycznych grzybic systemowych. Candida albicans jest drobnoustrojem polimorficznym. Zmiana fenotypu związana jest z oddziaływaniem czynników środowiskowych. Dzięki zdolnościom polimorficznym formy drożdżopodobne mogą przeciwstawić się mechanizmom fagocytozy. Celem pracy jest ocena stężenia ATP (adenosine triphosphate) w procesie tworzenia form kiełkujących przez C. albicans.

Materiał i metody

Do badań wykorzystano wzorcowy szczep C. albicans ATCC 10231. W pomiarach stężenia ATP wykorzystano ATP Assay Kit firmy LKB Wallac. Do oceny liczby komórek użyto urządzenia Scepter firmy Merck Millipore oraz densytometru DensiLaMeter II.

Wyniki

Stężenie ATP po 120 min było wyższe w przypadku komórek C. albicans stymulowanych L-proliną oraz D-glukozą niż w komórkach niestymulowanych. Największe stężenie ATP występowało w komórkach C. albicans poddanych stymulacji L-proliną. Również w tym przypadku występowała największa liczba form kiełkujących.

Wnioski

1. Tworzeniu form kiełkujących C. albicans towarzyszy wzrost stężenia ATP w pojedynczej komórce.
2. Niezależnie od rodzaju substancji stymulującej w procesie tworzenia form kiełkujących w C. albicans dochodzi do wzrostu stężenia ATP w przeliczeniu na pojedynczą komórkę grzyba.
Efficacy of EMDR Therapy for Children With PTSD: A Review of the Literature
Authors: Beer Renée
Published: November 2018

The rationale is synthesized for the urgency of empirical studies demonstrating the efficacy of eye movement desensitization and reprocessing (EMDR) therapy for children and adolescents with posttraumatic stress disorder (PTSD), symptoms of PTSD, or other trauma-related symptoms. This literature review examined 15 studies (including nine randomized clinical trials) that tested the efficacy of EMDR therapy for the treatment of children and adolescents with these symptoms. All studies found that EMDR therapy produced significant reductions in PTSD symptoms at posttreatment and also in other trauma-related symptoms, when measured. A methodological analysis identified limitations in most studies, reducing the value of these findings. Despite these shortcomings, the methodological strength of the identified studies has increased over time. The review also summarized three meta-analyses. The need for additional rigorous research is apparent, and in order to profit from experiences of the past, the article provides some guidelines for clinicians seeking to conduct future research in their agencies.