bluuebunny/crossref_metadata_2025 huggingface dataset:
MYD88 L265P Somatic Mutation
Authors: Ondrejka Sarah L. , Lin Jeffrey J. , Warden Doug W. , Durkin Lisa , Cook James R. , Hsi Eric D.
Published: August 2013

Objectives:

To examine the usefulness of the MYD88 L265P somatic mutation in identifying cases of lymphoplasmacytic lymphoma (LPL) from other lymphoplasmacytic neoplasms in bone marrow biopsy specimens.

Methods:

We studied 64 bone marrow biopsy specimens with involvement by various small B-cell lymphomas or plasma cell myeloma.

Results:

The MYD88 L265P somatic mutation was present in 13/13 cases of LPL, 1/13 cases of hairy cell leukemia, and absent in the other mature B-cell neoplasms tested. A test set of diagnostically challenging bone marrow cases with lymphoplasmacytoid morphology (B-cell lymphoma, not otherwise specified) was selected for additional review and reclassified, without knowledge of the MYD88 L265P status. Of those 16 cases, 7 were positive for MYD88, including 4/4 cases that were reclassified as LPL during the review.

Conclusions:

Although not entirely specific, MYD88 L265P is a useful adjunct for bone marrow diagnosis in separating LPL from other small B-cell lymphomas and plasma cell myeloma.
B-Cell Transcription Factor Expression and Immunoglobulin Gene Rearrangement Frequency in Acute Myeloid Leukemia With t(8;21)(q22;q22)
Authors: Johnson Ryan C. , Ma Lisa , Cherry Athena M. , Arber Daniel A. , George Tracy I.
Published: August 2013

Objectives:

To assess a large series of patients with acute myeloid leukemia (AML) with t(8;21) for both IGH@ and IGK@ B-cell gene rearrangements and for expression of PAX5, OCT2, and Bob.1 by immunohistochemistry and expression of CD19, CD79a, CD20, and CD22 by flow cytometry immunophenotyping.

Methods:

A total of 48 cases of AML with t(8;21)(q22;q22) were evaluated by immunohistochemistry and/or heavy chain and light chain immunoglobulin rearrangement studies where paraffin-embedded and/or fresh frozen material was available for study; previously performed flow cytometry studies were also reviewed in available cases.

Results:

Our study yielded 1 of 19 cases of AML with t(8;21) with an IGH@ gene rearrangement; blasts were associated with weak PAX5 expression. In addition, expression of antigens CD79a by flow cytometry and OCT2 by immunohistochemistry were highly associated with PAX5 expression, and CD19 was expressed in most cases assessed.

Conclusions:

Although B-cell antigen and B-cell transcription factor expression is seen in the majority of AMLs with t(8;21)(q22;q22) and correlates with PAX5 expression, immunoglobulin gene rearrangements are an uncommon event in this group of leukemias.
Analysis of Cutaneous Lymphomas in a Medical Center in Bahia, Brazil
Authors: Bittencourt Achiléa L. , Oliveira Pedro D. , Andrade Agnes C. , Santos Tamara C. , Oliveira Rodrigo F. , Farré Lourdes , Araujo Iguaracyra
Published: August 2013

Objectives:

To evaluate the frequency of the different types of cutaneous lymphoma (CL) in 1 university hospital in Brazil and compare this frequency with those observed in other countries.

Methods:

After review, 72 (84.7%) cases of primary cutaneous T-cell lymphoma (CTCL) and 13 (15.3%) cases of primary cutaneous B-cell lymphoma (CBCL) were included.

Results:

Of the CTCLs, 40.3% were mycosis fungoides (MF); 26.4% were adult T-cell leukemias/lymphomas (ATLs); 23.6% were peripheral T-cell lymphomas, unspecified; and 8.3% were anaplastic large cell lymphomas. Of the MF cases, 17.2% progressed to transformed MF. Five-year survival for primary human T-cell lymphotropic virus type 1–negative CTCL, ATL, and CBCL was 64.0%, 42.1%, and 62.5%, respectively. MF and ATL were the most frequent primary CTCLs.

Conclusions:

The frequencies observed here are close to those observed in Peru but different from those of European countries. Unfortunately, the World Health Organization/ European Organization of Research and Treatment of Cancer classification does not include primary cutaneous ATL.
The Semantics of Partitive Constructions The definite vs. indefinite contrast in Italian and Latin
Authors: Casadio Claudia
Published: August 2013

This paper investigates the semantic properties of partitive constructions in Italian and Latin. Two basic realizations of partitivity are discussed, depending on the way the partition operation is performed on noun phrase denotations, that is, depending on the characterization of the part-whole relation: they will be called
Attivo e deponente nei verbi in -fico(r) a primo elemento sostantivale
Authors: Marini Emanuela
Published: August 2013

In the first section of this article I see that in
Die etymologische Herleitung des Namens Latium
Authors: Adamik Béla
Published: August 2013

The aim of this study is to refute the standard etymology of the placename
Semantic Roles within a Contrastive Grammar of Latin and German
Authors: Kienpointner Manfred
Published: August 2013

This paper deals with the problem of defining and describing semantic roles. Methodologically, I follow Coseriu (1987), who argues for a sharp distinction between language-independent reference roles and language-specific semantic roles and criticizes earlier approaches which do not make this distinction. Recent contributions to the problem of defining and describing semantic roles or functions within Generative Grammar (Jackendoff 1991) and Functional Grammar (Dik 1997) differ from earlier approaches in that they try to take this distinction into account However, some problems remain. When we have to deal with syntactic and semantic contrasts between languages such as Latin and German, these contrasts cannot adequately be described with the help of language-independent theta-roles or semantic functions. Therefore, it seems preferable to adopt Coseriu's strategy of describing the expression of reference roles like 'Possessor' in Latin and German as a set of contrasting contextual meanings of cases, syntactic functions and constructions. Contrastive data show that the relevant constructions in Latin and German differ widely both as far as their number and their functional properties are concerned.
Champ lexical et polysémie : l'expression du pardon en latin
Authors: Moussy Claude
Published: August 2013

The aim of this paper is to investigate a Latin lexical field (the field for forgiveness) which has progressively increased. In addition to
¿Accusativus pendens en Latín?
Authors: Álvarez Huerta Olga
Published: August 2013

This paper concerns certain uses of the Latin accusative where it does not behave as an object, but as a subject. Some of these Accusatives, especially the proleptic accusative and the exclamative accusative, seem to share some of their most defining characteristics with the construction known as
Word length and clitics in Latin
Authors: Babič Matjaz
Published: August 2013

This paper presents a possible approach to research on Latin clitics that is based mostly on statistic observations. The research was performed by observations on frequencies of different word lengths in various Latin texts. The results show a quantitative predominance of shorter words in Latin texts of more colloquial nature. They also demonstrate that shorter words in Latin have a predominantly grammatical function.
Saying NO to lam
Authors: Huitink Luuk
Published: August 2013

In this contribution the linguistic behaviour of
II congiuntivo nella proposizione relativa nominalizzata (Cic. Verr. II)
Authors: Lavency Marius
Published: August 2013

"Nominalisierter Relativsau" (NR) heißt hier der Relativsatz, der auf kein lexikalisiertes Normen bezogen ist, eine Gesamtheit von Wesen
Corrélation
Authors: Touratier Christian
Published: August 2013

We call correlation two different syntactic structures: First, when a subordinate clause is followed by a main clause beginning with a grammatical constituent of the same meaning as the subordinating conjunction. Then when a syntactic structure consists of two parallel clauses none of which seems to contain a subordinating morpheme, but both of which begin with a grammatical constituent of the same meaning. The first structure is a particular case of Subordination, the second is a particular case of juxtaposition, i. e. of coordination without a coordinating conjunction. Nevertheless both structures share a syntagmatic feature and an informative one.
Bemerkungen zum Gebrauch von Präpositionen im klassischen Latein
Authors: Weische Alfons
Published: August 2013

By prepositions people can more precisely describe complex relations of persons and things than by means of pure cases. Precision was especially necessary in Roman society in the last decades of the Republic, when the economy and jurisprudence flourished. As a response to these growing demands, certain anonymous speakers created an elaborate system of prepositions in the common language. Through this way of giving information the already-existing tendency to pronounce the endings of cases in a lax manner became less troublesome. In relation to modern developments of common language Cicero and Caesar exercised restraint, following their ideal of
De quelques emplois de copia chez Plaute
Authors: Crampon Monique
Published: August 2013

Among the terms which mean “help” in Plautus’ corpus, copia occupies a characteristic place and can be understood either as a metonymy - when the servus callidus is called help - or as a metaphor - when the same slave is assimilated to the goddess Copia, who may have been worshipped in Rome -. These occurrences of Copia, which are always observed at very specific times in the plays, either at the beginning or at the end of a scene, are often emphasized by stylistic features such as sound effects, a picturesque style or puns on words. Moreover, such sentences, articulated on the word copia con- tribute to the “animation of inanimate things” which is so typical of Plautus’ art.
Cicero and the Art of Translation
Authors: Kopeczky Rita
Published: August 2013

Cicero makes it plain in his remarks on translation that he holds lexical operations as an acceptable way of procedure for a competent translator (contrary to interpretes indiserti). This is supported by the methodological analysis of his translations. Comparing a prose translation and a poetical one, we can show the relative frequency of different lexical operations in their connection with the requirements of the genres, and with the predilections of the translator as well. We can find that these are used not only when the systemic differences between source and target language make them necessary, but they also become a means of aemulatio with the source text, which, by Cicero’s age, had become an essential component of artistic translation.
Los capitulos como unidades textuales. Enunciados temáticos + quippe en Veleyo Patérculo, H.R. 2.1-57
Authors: Castellanos Antonio Ruit
Published: August 2013

This study stays at the crossroads of Rhetoric, Text Grammar and Textual Critic. It studies the division of chapters (made firstly by Gruter in 1607) of the Roman History (Historic Romwia: H.R.) of Velleius Paterculus: the old Rhetoric text types, the opening and closing marks, the characters and personages and the thematic sentences as cohesive devices. These thematic sentences are used as introductions of chapter, and they are followed by confirmatives with quippe and with demonstrative relatives. They constitute a mark similar to the propositions of the argumentative texts, with special syntactic characteristics in the text of Velleius. The studied corpus are the first 57 chapters of the second book of the Historia Romana.
XML e lo studio dei manoscritti. Linee di orientamento e un esempio di applicazione
Authors: Milanese Guido
Published: August 2013

La diffusione dell'uso di XML è senza dubbio uno dei fenomeni più interessanti nel trattamento dell'informazione che si sia riscontrato negli ultimi anni. Nato alla fine degli anni '90 come successore di SGML, XML rappresenta oggi una realtà in notevolissimo sviluppo, anche se non è riuscito a "colonizzare" il Web come ci si sarebbe aspettati: il Web rimane ancora largamente terreno popolato da pagine HTML scritte in modo approssimativo e che rendono molto diffìcile il raggiungimento e la gestione della conoscenza. Dopo una illustrazione essenziale della struttura fondamentale di XML, questo articolo si propone di illustrare le potenzialità offerte da XML alla catalogazione dei manoscritti e in generale allo studio paleografico, fornendo un esempio tratto da un lavoro recentemente pubblicato, seguendone lo sviluppo completo dalla progettazione di uno schema descrittivo sino alla realizzazione dell'output grafico.
A new low temperature route to uranyl borates with structural variations
Authors: Wu Shijun , Wang Shuao , Polinski Matthew J. , Depmeier Wulf , Albrecht-Schmitt Thomas E. , Alekseev Evgeny V.
Published: August 2013

Three new uranyl borates, K(UO
Syntheses, structures, and characterization of two new rubidium cadmium(II)/zinc(II) borates: Rb3MB5O10 (M = Cd, Zn)
Authors: Yu Hongwei , Wu Hongping , Pan Shilie , Dong Lingyun , Han Shujun , Jiao Anqing , Poeppelmeier Kenneth R.
Published: August 2013

Two new ternary borates, Rb
Em busca do feto saudavel: ideias, marcas e coisas na reconstrucao do diagnostico da toxoplasmose
Authors: Silva Vera Lucia Marques da , Camargo Junior Kenneth Rochel
Published: August 2013

Resulta da investigação do atendimento médico à gestante na busca do feto saudável, especificamente da operacionalização do diagnóstico e da tomada de decisão quanto ao objeto infecção-doença toxoplasmose. Seguindo a filosofia empírica e Annemarie Mol, esse objeto seria 'manipulado', pelo menos, por médico e paciente. Tal manipulação corresponde a uma reconstrução ontológica, a partir da articulação dos elementos ideias-marcas-coisas no contexto espaçotemporal, o que propicia a multiplicação do objeto. Pretende-se contribuir para a teorização da política ontológica considerando que a multiplicação do objeto e as várias intervenções decorrentes implicaram diferentes modelagens dos corpos e deslocamentos de vida.
Ensino de ciências: realismo, antirrealismo e a construção do conceito de oxigênio
Authors: Silva Marcos Rodrigues da
Published: August 2013

Pesquisas em ensino de ciências têm apontado a importância de uma discussão sobre a natureza da ciência. Um meio de se veicular uma concepção mais real da natureza da ciência seria os professores adotarem uma atitude filosófica sobre os conceitos que denotam entidades inobserváveis. No caso de conceitos já abandonados pelas teorias científicas - como o de flogisto - é comum apresentar que foram descartados porque nada representaram empiricamente. Porém, na época da utilização do conceito de flogisto, ele se inseria numa rede teórica que era explicativa e justificava a sua adoção. Este artigo defende a ideia de que a adoção de posições filosóficas perante conceitos científicos precisa ser respaldada pelo conhecimento acerca da história do conceito.
The Impact of Emotional, Physical, and Sexual Abuse on Contraceptive Method Selection and Discontinuation
Authors: Allsworth Jenifer E. , M. Secura Gina , Zhao Qiuhong , Madden Tessa , Peipert Jeffrey F.
Published: August 2013

Objectives. We evaluated the impact of exposure to emotional, physical, or sexual abuse on contraceptive method selection and discontinuation.

Methods. We performed a secondary analysis of 7170 women enrolled in the Contraceptive CHOICE Project in St. Louis, Missouri, a prospective cohort study in which 9256 women were provided their preferred method of contraception at no cost from 2007 to 2011. We defined contraceptive discontinuation as device removal or nonuse for at least 4 weeks within the first 12 months after initiation.

Results. One third of women experienced some abuse in their lifetimes. Women with an abuse history were as likely as those without to select a long-acting reversible contraceptive method and more likely to choose a contraceptive injection, the patch, or the ring. When we compared women who were abused to those who were not, rates of discontinuation at 12 months were higher among women who selected long-acting reversible contraception (17% vs 14%; P = .04) and significantly higher among women who selected non–long-acting methods (56% vs 47%; P < .001). Type of abuse did not alter the association between abuse and contraceptive continuation.

Conclusions. Previous experiences of abuse are associated with both contraceptive method selection and continuation.
Only Your Calamity: The Beginnings of Activism by and for People With AIDS
Authors: Wright Joe
Published: August 2013

The invention of AIDS activism came soon after the AIDS epidemic emerged in gay communities in the United States in the early 1980s. AIDS activism by and for people with AIDS, distinct from gay activism responding to the threat of AIDS on the behalf of the whole community, started as a way of resisting the phenomenon of social death. Social death, in which people are considered “as good as dead” and denied roles in community life, posed a unique threat to people with AIDS. An organized political response to AIDS began among gay men with AIDS in San Francisco, California, and New York, New York, formalized in a foundational document later called the Denver Principles. The ideas and language of these first people with AIDS influenced later AIDS activism movements. They also help to illustrate the importance of considering an epidemic from the point of view of people with the disease.
Prevalence of Gender Identity Disorder and Suicide Risk Among Transgender Veterans Utilizing Veterans Health Administration Care
Authors: Blosnich John R. , Brown George R. , Shipherd, PhD Jillian C. , Kauth Michael , Piegari Rebecca I. , Bossarte Robert M.
Published: August 2013

Objectives. We estimated the prevalence and incidence of gender identity disorder (GID) diagnoses among veterans in the Veterans Health Administration (VHA) health care system and examined suicide risk among veterans with a GID diagnosis.

Methods. We examined VHA electronic medical records from 2000 through 2011 for 2 official ICD-9 diagnosis codes that indicate transgender status. We generated annual period prevalence estimates and calculated incidence using the prevalence of GID at 2000 as the baseline year. We cross-referenced GID cases with available data (2009–2011) of suicide-related events among all VHA users to examine suicide risk.

Results. GID prevalence in the VHA is higher (22.9/100 000 persons) than are previous estimates of GID in the general US population (4.3/100 000 persons). The rate of suicide-related events among GID-diagnosed VHA veterans was more than 20 times higher than were rates for the general VHA population.

Conclusions. The prevalence of GID diagnosis nearly doubled over 10 years among VHA veterans. Research is needed to examine suicide risk among transgender veterans and how their VHA utilization may be enhanced by new VA initiatives on transgender care.
Bioimpedance-Guided Fluid Management in Hemodialysis Patients
Authors: Moissl Ulrich , Arias-Guillén Marta , Wabel Peter , Fontseré Néstor , Carrera Montserrat , Campistol José Maria , Maduell Francisco
Published: August 2013

Background and objectives

Achieving and maintaining optimal fluid status remains a major challenge in hemodialysis therapy. The aim of this interventional study was to assess the feasibility and clinical consequences of active fluid management guided by bioimpedance spectroscopy in chronic hemodialysis patients.

Design, setting, participants, & measurements

Fluid status was optimized prospectively in 55 chronic hemodialysis patients over 3 months (November 2011 to February 2012). Predialysis fluid overload was measured weekly using the Fresenius Body Composition Monitor. Time-averaged fluid overload was calculated as the average between pre- and postdialysis fluid overload. The study aimed to bring the time-averaged fluid overload of all patients into a target range of 0.5±0.75 L within the first month and maintain optimal fluid status until study end. Postweight was adjusted weekly according to a predefined protocol.

Results

Time-averaged fluid overload in the complete study cohort was 0.9±1.6 L at baseline and 0.6±1.1 L at study end. Time-averaged fluid overload decreased by −1.20±1.32 L (P<0.01) in the fluid-overloaded group (n=17), remained unchanged in the normovolemic group (n=26, P=0.59), and increased by 0.59±0.76 L (P=0.02) in the dehydrated group (n=12). Every 1 L change in fluid overload was accompanied by a 9.9 mmHg/L change in predialysis systolic BP (r=0.55, P<0.001). At study end, 76% of all patients were either on time-averaged fluid overload target or at least closer to target than at study start. The number of intradialytic symptoms did not change significantly in any of the subgroups.

Conclusions

Active fluid management guided by bioimpedance spectroscopy was associated with an improvement in overall fluid status and BP.
The LA aggression scale for elementary school and upper secondary school students: Examination of psychometric properties of a new multidimensional measure of self- reported aggression
Authors: Kozina Ana
Published: August 2013

The paper introduces a new multidimensional scale LA (lestvica agresivnosti
[Aggression Scale]) for measuring self-reported aggression in elementary and
upper secondary school students in Slovenia. The scale has been developed
with a special focus on the school setting, using three elementary school
samples (preliminary study: N=2777; main study: N=10427 and validity and
test-retest study: N=191) representative of 4th and 8th grade students in
Slovenia and one upper secondary school sample (N=3253) representative of the
final year of upper secondary school in Slovenia. The exploratory analyses
using principal component analyses (PCA) revealed a four-factor structure:
verbal aggression, physical aggression, internal aggression and aggression
towards authority that were interrelated. Confirmatory factor analyses (CFA)
showed that the items of the scale formed four factors that were related to
the higher order factor. The structure turned out to be stable over different
age groups. The scale demonstrated adequate internal consistency, concurrent
validity and test-retest stability.
Socijalni, psiholoski i ideoloski koreni nacionalistickih stavova u Srbiji
Authors: Todosijevic Bojan
Published: August 2013

nema
Mood congruence effect in autobiographical recall: Is mood a mediator?
Authors: Drace Sasa , Desrichard Olivier
Published: August 2013

In the present study we test the hypothesis that the effect of mood
congruence in autobiographical recall is underlain by mood. Thirty-eight
female participants were subjected to positive, negative and neutral mood
inductions, and then asked to recall three personal memories. Participants?
mood was assessed using self-report questionnaires and by electromyograph
(EMG) measurements of corrugator supercilii and zygomaticus major muscle
activity. We replicated the congruence effect between the mood inductions and
the valence of the participants? recalled memories. Furthermore, this effect
was mediated by mood, as measured by EMG and self-report questionnaires. The
results suggest that mood influences the mood congruence effect in a way that
cannot be explained by semantic priming alone.
Impact of different conditions on accuracy of five rules for principal components retention
Authors: Zoric Aleksandar , Opacic Goran
Published: August 2013

Polemics about criteria for nontrivial principal components are still present
in the literature. Finding of a lot of papers, is that the most frequently
used Guttman Kaiser?s criterion has very poor performance. In the last three
years some new criteria were proposed. In this Monte Carlo experiment we
aimed to investigate the impact that sample size, number of analyzed
variables, number of supposed factors and proportion of error variance have
on the accuracy of analyzed criteria for principal components retention. We
compared the following criteria: Bartlett?s ?2 test, Horn?s Parallel
Analysis, Guttman-Kaiser?s eigenvalue over one, Velicer?s MAP and CHull
originally proposed by Ceulemans & Kiers. Factors were systematically
combined resulting in 690 different combinations. A total of 138,000
simulations were performed. Novelty in this research is systematic variation
of the error variance. Performed simulations showed that, in favorable
research conditions, all analyzed criteria work properly. Bartlett?s and
Horns criterion expressed the robustness in most of analyzed situations.
Velicer?s MAP had the best accuracy in situations with small number of
subjects and high number of variables. Results confirm earlier findings of
Guttman-Kaiser?s criterion having the worse performance.
Stratified Patient-Centered Care in Type 2 Diabetes
Authors: Slingerland Annabelle S. , Herman William H. , Redekop William K. , Dijkstra Rob F. , Jukema J. Wouter , Niessen Louis W.
Published: August 2013

OBJECTIVE

Diabetes treatment should be effective and cost-effective. HbA1c-associated complications are costly. Would patient-centered care be more (cost-) effective if it was targeted to patients within specific HbA1c ranges?

RESEARCH DESIGN AND METHODS

This prospective, cluster-randomized, controlled trial involved 13 hospitals (clusters) in the Netherlands and 506 patients with type 2 diabetes randomized to patient-centered (n = 237) or usual care (controls) (n = 269). Primary outcomes were change in HbA1c and quality-adjusted life years (QALYs); costs and incremental costs (USD) after 1 year were secondary outcomes. We applied nonparametric bootstrapping and probabilistic modeling over a lifetime using a validated Dutch model. The baseline HbA1c strata were <7.0% (53 mmol/mol), 7.0–8.5%, and >8.5% (69 mmol/mol).

RESULTS

Patient-centered care was most effective and cost-effective in those with baseline HbA1c >8.5% (69 mmol/mol). After 1 year, the HbA1c reduction was 0.83% (95% CI 0.81–0.84%) (6.7 mmol/mol [6.5–6.8]), and the incremental cost-effectiveness ratio (ICER) was 261 USD (235–288) per QALY. Over a lifetime, 0.54 QALYs (0.30–0.78) were gained at a cost of 3,482 USD (2,706–4,258); ICER 6,443 USD/QALY (3,199–9,686). For baseline HbA1c 7.0–8.5% (53–69 mmol/mol), 0.24 QALY (0.07–0.41) was gained at a cost of 4,731 USD (4,259–5,205); ICER 20,086 USD (5,979–34,193). Care was not cost-effective for patients at a baseline HbA1c <7.0% (53 mmol/mol).

CONCLUSIONS

Patient-centered care is more valuable when targeted to patients with HbA1c >8.5% (69 mmol/mol), confirming clinical intuition. The findings support treatment in those with baseline HbA1c 7–8.5% (53–69 mmol/mol) and demonstrate little to no benefit among those with HbA1c <7% (53 mmol/mol). Further studies should assess different HbA1c strata and additional risk profiles to account for heterogeneity among patients.
Genetic variability of microsatellites in autochthonous Podolian cattle breeds in Croatia
Authors: Keros Tomislav , Jemeršić Lorena , Prpić Jelena , Benić Miroslav , Roić Besi , Brnić Dragan
Published: August 2013

The aim of the study was to analyse the status of the genetic structure of two autochthonous Croatian Podolian breeds (Istrian cattle and Slavonian Syrmian Podolians). The study comprised 20 randomly selected blood samples of each cattle breed. The genotyping was performed on 11 microsatellites (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, BM1824) as recommended by International Society of Animal Genetics and Food and Agriculture Organization. The procedure enabled the identification of 423 (96.14%) genotypes, indicating the validity of DNA analyses in blood samples and adequacy of the selected microsatellite markers. Likewise, there was the total number of 203 alleles with minimal differences among the studied groups. The differences (p> 0.05)were observed within the genotypes of the studied groups regarding the microsatellite loci. The distribution of the genetic relationship was presented by Wright F coefficients. The FIShad negative values for the TGLA 227 locus in both studied groups and for ETH3, ETH225 and BM1834 loci in the Slavonian Syrmian Podolians. This obtained data with relative high values of heterozygosity also shows a genetical influence of other breeds. Almost all FITvalues were appropriate (0.31905) as a result of excessive selection effects. The mean value of FST(0.07336) indicates that the average degree of genetic variability of the studied breeds was not significantly different despite certain differences in individual loci. The observed specific polymorphism of loci shows that the Istrian cattle and the Slavonian Syrmian Podolians have similar genetic characteristics, but also that there are certain differences between the breeds and even inter-breed differences. The findings indicate a relationship between the breeds and their suitable purity for interbreeding but they also indicate certain danger to certain parts of genome within the studied species. The results can be applied in the protection and breeding programs for the preservation of autochtonous Croatian Podolian breeds.
Effect of β-1,3/1,6-D-glucan in diet on productivity and humoral and cellular defense mechanisms in sheep
Authors: Ząbek Katarzyna , Milewski Stanisław , Wójcik Roman , Siwicki Andrzej Krzysztof
Published: August 2013

The aim of this study was to determine the effect of β-1,3/1,6-D-glucan, isolated fromSaccharomyces cerevisiae, on indicators of milk and meat performance in sheep as well as on selected non-specific indicators of humoral and cellular defense. The experiment was carried on 26 suckling ewes divided into 2 equal groups, and their offspring (21 in each group). The ewes were administered concentrate with the addition of β-1,3/1,6-D-glucan at a dose of 3 g/kg. Indicators of milk performance and markers of humoral and cellular immunity were analyzed on days 28 and 70 of lactation; and the indicators of meat performance of lambs on day 28 and 70 of their life. The addition of β-1,3/1,6-D-glucan was observed to cause an increase in milk performance by 13.5–14%. Simultaneously, milk was characterized by a lower somatic cell count. Diet supplementation had a positive effect on the chemical composition of milk, which was manifested by increased percentage contents of fat (by 15– 30%) and protein (by 11%). Lambs were characterized by a higher growth rate and better muscle tissue development. The supplementation caused an increase of gamma-globulin concentration (by 6.33–9.5 g/l), lysozyme activity (by 0.1 mg/l), respiratory burst activity (by 0.11–0.14), potential killing activity (by 0.10–0.12), proliferative response of T-cells stimulated by mitogen concanavalineA (by 0.07–0.09 RI) and proliferative response of B-cells stimulated by mitogen lipopolysaccharide (by 0.13–0.16 RI) in sheep’s blood. The activity of β-1,3/1,6-D-glucan as a natural immunostimulator has been studied in many animal species, however, this is the first study conducted on sheep.
Effects of tramadol with tiletamine/zolazepam-xylazine as anaesthesia in cats
Authors: Li Lin , Dong Jing , Lu Dezhang , Jiang Sheng , Lin Dongqi , Fan Honggang
Published: August 2013

This study was conducted to investigate the effect of the combination of tiletamine/zolazepam-xylazine-tramadol (TZXT) in cats and to compare its efficacy as an anaesthetic technique with tiletamine/zolazepam-xylazine (TZX). Fourteen healthy thirteen-month-old cats of both sexes were immobilized by TZXT or TZX on two different occasions. The cats’ scores for immobilization, analgesia and baseline physiological indicators, heart rate, respiratory rate and rectal temperature, were recorded before and 5, 10, 15, 20, 30, 40, 50, 60, 80, 100 and 120 min after administration of TZXT or TZX. Animals in both groups became laterally recumbent within 3 min and physiological indicators changed after administration. These changes were within biologically acceptable limits; these measurements did not differ between the treatments. Induction and recovery quality in animals was better with TZXT than with TZX. Sedation, analgesia, muscle relaxation and posture scores was higher in TZXT group. During recovery, cats in TZXT group were more conscious than cats in TZX group. This study showed that in cats, addition of tramadol (T) to TZX improved the quality of anaesthetic induction and increased the analgesic effect without adversely affecting the physiological indicators. Thus tiletamine/zolazepam-xylazine-tramadol shows to be more suitable for cat anaesthesia.
Pandectism and the Gaian Classification of Things
Authors: Giglio Francesco
Published: August 2013

Gaius was the first Roman jurist to classify legal things into corporeal and incorporeal. His influential classification did not expressly mention the right of ownership. The German Pandectists justified this absence arguing that the list of tangibles referred to the objects of ownership. Following Pandectism, many modern legal systems acknowledge ownership only with regard to corporeal things. This paper departs from the Pandectists' conclusions. The categorization at issue should be read as part of Gaius's effort to educate his students for the legal profession. Gaius proposed a coherent classification embedded into a clear didactic project. The jurist was not seeking to advance a theory of ownership, but to link the legal actions to their objects.
“Horseshoe cord terminus” sans filum around a bone spur: a rare composite of faulty gastrulation with agenesis of secondary neurulation
Authors: Dhandapani Sivashanmugam , Mehta Veer S. , Sharma Bhavani S.
Published: August 2013

Split cord malformation (SCM) is classified based on the presence of a bone spur and double dural sac. The authors report on a 6-year-old child with primary enuresis in whom MRI findings were suggestive of Type I SCM, and who had unique intraoperative findings of a horseshoe-shaped split cord terminus anchored by a bone spur without the normally tapering conus and filum. The typical appearance of cauda equina was absent, with all the roots arising from the horseshoe cord terminus. This composite anomaly is probably due to the rare combination of faulty gastrulation with abnormal persistence of endomesenchymal tract causing SCM, with concurrent agenesis of secondary neurulation in turn causing absence of filum.
A Simple Algorithm for Solving for the Generalized Longest Common Subsequence (LCS) Problem with a Substring Exclusion Constraint
Authors: Zhu Daxin , Wang Xiaodong
Published: August 2013

This paper studies the string-excluding (STR-EC)-constrained longest common subsequence (LCS) problem, a generalized LCS problem. For the two input sequences, X and Y , of lengths n andmand a constraint string, P, of length r, the goal is to find the longest common subsequence, Z, of X and Y that excludes P as a substring. The problem and its solution were first proposed by Chen and Chao, but we found that their algorithm cannot solve the problem correctly. A new dynamic programming solution for the STR-EC-LCS problem is then presented in this paper, and the correctness of the new algorithm is proven. The time complexity of the new algorithm is O(nmr).
Pyranose Dehydrogenase from Agaricus campestris and Agaricus xanthoderma: Characterization and Applications in Carbohydrate Conversions
Authors: Staudigl Petra , Krondorfer Iris , Haltrich Dietmar , Peterbauer Clemens
Published: August 2013

Pyranose dehydrogenase (PDH) is a flavin-dependent sugar oxidoreductase that is limited to a rather small group of litter-degrading basidiomycetes. The enzyme is unable to utilize oxygen as an electron acceptor, using substituted benzoquinones and (organo) metal ions instead. PDH displays a broad substrate specificity and intriguing variations in regioselectivity, depending on substrate, enzyme source and reaction conditions. In contrast to the related enzyme pyranose 2-oxidase (POx), PDHs from several sources are capable of oxidizing α- or β-1→4-linked di- and oligosaccharides, including lactose. PDH from A. xanthoderma is able to perform C-1 and C-2 oxidation, producing, in addition to lactobionic acid, 2-dehydrolactose, an intermediate for the production of lactulose, whereas PDH from A. campestris oxidizes lactose nearly exclusively at the C-1 position. In this work, we present the isolation of PDH-encoding genes from A. campestris (Ac) and A. xanthoderma (Ax) and a comparison of other so far isolated PDH-sequences. Secretory overexpression of both enzymes in Pichia pastoris was successful when using their native signal sequences with yields of 371 U·L−1 for AxPDH and 35 U·L−1 for AcPDH. The pure enzymes were characterized biochemically and tested for applications in carbohydrate conversion reactions of industrial relevance.
Explaining Differential Reporting of Victimization between Parents and Children: A Consideration of Social Biases
Authors: John Sufna , DiLalla Lisabeth
Published: August 2013

Studies have shown that children and parents provide different reports of children’s victimization, with children often reporting more victimization. However, the reason for this differential reporting is unclear. This study explored two types of social biases (emotion recognition and perceived impairment) in parents and children as possible reasons underlying differential reporting. Six- to 10-year-old children and one of their parents were tested in a lab. Testing included subjective measures of parent alexithymic traits, child perceived impairment from victimization, and child- and parent-reported frequency of children’s peer victimization and internalizing and externalizing difficulties. Parents and children also completed an objective measure of emotion recognition. Both types of social bias significantly predicted reports of children’s peer victimization frequency as well as internalizing and externalizing difficulties, as rated by parents and children. Moreover, child perceived impairment bias, rather than parent emotion bias, best predicted differential reporting of peer victimization. Finally, a significant interaction demonstrated that the influence of child perceived impairment bias on differential reporting was most salient in the presence of parent emotion bias. This underscores the importance of expanding interventions for victimized youth to include the restructuring of social biases.
Tree Species Richness and Stand Productivity in Low-Density Cluster Plantings with Oaks (Quercus robur L. and Q. petraea (Mattuschka) Liebl.)
Authors: Saha Somidh , Kuehne Christian , Bauhus Jürgen
Published: August 2013

Low density plantings complemented by natural regeneration is an increasingly common reforestation technique to ensure growth of a sufficient number of trees from desired species while maintaining natural processes such as succession. One such form of low density planting that aims at lowering establishment costs—oak clusters—has been developed as an alternative to row planting since the 1980s in central Europe. However, whether cluster planting provides higher species richness and productivity than high density row planting has not previously been analyzed. Here, we compare tree species richness and productivity (measured as stand basal area) between oak cluster plantings and conventional row planting in young (10–26 years old) forest stands at seven study sites in Germany. Tree species richness was significantly higher in cluster plantings than in row plantings, whereas total basal areas were comparable. Naturally regenerated trees contributed on average to 43% of total stand basal area in cluster plantings, which was significantly higher than in row plantings. Total stand basal area in cluster planting was significantly related to the density of naturally regenerated trees. In turn, tree species diversity, density and basal area of naturally regenerated trees were increased with the size of unplanted area between clusters. Our results demonstrate that the admixture of naturally regenerated, early and mid-successional tree species compensates for a possible loss in productivity from planting fewer oaks. Low density cluster plantings can offer significant environmental benefits, at least for the first few decades of stand development, without compromising productivity.
Trastuzumab-Peptide Interactions: Mechanism and Application in Structure-Based Ligand Design
Authors: Sun Tian-Yang , Wang Qi , Zhang Jin , Wu Tao , Zhang Fan
Published: August 2013

Understanding of protein-ligand interactions and its influences on protein stability is necessary in the research on all biological processes and correlative applications, for instance, the appropriate affinity ligand design for the purification of bio-drugs. In this study, computational methods were applied to identify binding site interaction details between trastuzumab and its natural receptor. Trastuzumab is an approved antibody used in the treatment of human breast cancer for patients whose tumors overexpress the HER2 (human epidermal growth factor receptor 2) protein. However, rational design of affinity ligands to keep the stability of protein during the binding process is still a challenge. Herein, molecular simulations and quantum mechanics were used on protein-ligand interaction analysis and protein ligand design. We analyzed the structure of the HER2-trastuzumab complex by molecular dynamics (MD) simulations. The interaction energies of the mutated peptides indicate that trastuzumab binds to ligand through electrostatic and hydrophobic interactions. Quantitative investigation of interactions shows that electrostatic interactions play the most important role in the binding of the peptide ligand. Prime/MM-GBSA calculations were carried out to predict the binding affinity of the designed peptide ligands. A high binding affinity and specificity peptide ligand is designed rationally with equivalent interaction energy to the wild-type octadecapeptide. The results offer new insights into affinity ligand design.
The Association of IFI27 Expression and Fatigue Intensification during Localized Radiation Therapy: Implication of a Para-Inflammatory Bystander Response
Authors: Hsiao Chao-Pin , Araneta Maria , Wang Xiao , Saligan Leorey
Published: August 2013

The mechanisms behind fatigue intensification during cancer therapy remain elusive. The interferon alpha-inducible protein 27 (IFI27) was the most up-regulated gene based on our previous microarray data in fatigued men with non-metastatic prostate cancer receiving localized external beam radiation therapy (EBRT). The purpose of this study was to confirm the IFI27 up-regulation and determine its association with fatigue intensification during EBRT. Peripheral blood samples and fatigue scores were collected at three time points—prior to EBRT, at midpoint, and at completion of EBRT. Confirmatory quantitative real time polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA) were utilized to verify the microarray results. Subjects were a total of 40 Caucasian men with prostate cancer; 20 scheduled for EBRT (65.6 ± 7.5 years old), and 20 on active surveillance as controls (62.8 ± 6.1 years old). Significant IFI27 expression overtime during EBRT was confirmed by qPCR (p < 0.5), which correlated with fatigue scores during EBRT (R = −0.90, p = 0.006). Alterations in mechanisms associated with immune response and mitochondrial function that explain the up-regulation of IFI27 may provide an understanding of the pathways related to the intensification of fatigue during localized radiation therapy.
Synthesis, Structural Characterization, and Antitumor Activity of a Ca(II) Coordination Polymer Based on 1,6-Naphthalenedisulfonate and 4,4′-Bipyridyl
Authors: Tai Xishi , Zhao Wenhua
Published: August 2013

A novel Ca(II) coordination polymer, [CaL(4,4′-bipyridyl)(H2O)4]n (L = 1,6-naphthalenedisulfonate), was synthesized by reaction of calcium perchlorate with 1,6-naphthalenedisulfonic acid disodium salt and 4,4′-bipyridyl in CH3CH2OH/H2O. It was characterized by elemental analysis, IR, molar conductivity and thermogravimetric analysis. X-ray crystallography reveals that the Ca(II) coordination polymer belongs to the orthorhombic system, with space group P212121. The geometry of the Ca(II) ion is a distorted CaNO6 pengonal bipyramid, arising from its coordination by four water molecules, one nitrogen atom of 4,4′-bipyridyl molecule, and two oxygen atoms from two L ligands. The complex molecules form a helical chain by self-assembly. The antitumor activity of 1,6-naphthalenedisulfonic acid disodium salt and the Ca(II) coordination polymer against human hepatoma smmc-7721 cell line and human lung adenocarcinoma A549 cell line reveals that the Ca(II) coordination polymer inhibits cell growth of human lung adenocarcinoma A549 cell line with IC50 value of 27 μg/mL, and is more resistive to human lung adenocarcinoma A549 cell line as compared to 1,6-naphthalenedisulfonic acid disodium salt.
Eco-Friendly Synthesis and Antiproliferative Evaluation of Some Oxygen Substituted Diaryl Ketones
Authors: Arenas Paola , Peña Andrés , Ríos David , Benites Julio , Muccioli Giulio , Calderon Pedro , Valderrama Jaime
Published: August 2013

A broad variety of oxygen-substituted diaryl ketones has been synthesized by solar energy-induced Friedel Crafts acylations of 1,4-benzo- and 1,4-naphthoquinones with benzaldehydes. The in vitro antiproliferative properties of the photoproducts were assessed on prostate (DU-145), bladder (T24) and breast (MCF7) human-derived tumor cell lines and compared to non-tumor mouse fibroblasts (Balb/3T3). Among the tested compounds, it was found that those containing a 3,4,5-trimethoxyphenyl A-ring, such as 12 and 22 are more active on DU-145, with EC50 values of 1.2 and 5.9 μM, respectively. By comparing their effects on the three cancer cell lines, the analogue 22 has the best mean selective index (2.4).
ESB-Based Sensor Web Integration for the Prediction of Electric Power Supply System Vulnerability
Authors: Stoimenov Leonid , Bogdanovic Milos , Bogdanovic-Dinic Sanja
Published: August 2013

Electric power supply companies increasingly rely on enterprise IT systems to provide them with a comprehensive view of the state of the distribution network. Within a utility-wide network, enterprise IT systems collect data from various metering devices. Such data can be effectively used for the prediction of power supply network vulnerability. The purpose of this paper is to present the Enterprise Service Bus (ESB)-based Sensor Web integration solution that we have developed with the purpose of enabling prediction of power supply network vulnerability, in terms of a prediction of defect probability for a particular network element. We will give an example of its usage and demonstrate our vulnerability prediction model on data collected from two different power supply companies. The proposed solution is an extension of the GinisSense Sensor Web-based architecture for collecting, processing, analyzing, decision making and alerting based on the data received from heterogeneous data sources. In this case, GinisSense has been upgraded to be capable of operating in an ESB environment and combine Sensor Web and GIS technologies to enable prediction of electric power supply system vulnerability. Aside from electrical values, the proposed solution gathers ambient values from additional sensors installed in the existing power supply network infrastructure. GinisSense aggregates gathered data according to an adapted Omnibus data fusion model and applies decision-making logic on the aggregated data. Detected vulnerabilities are visualized to end-users through means of a specialized Web GIS application.