bluuebunny/crossref_metadata_2025 huggingface dataset:
MORE ZFC INEQUALITIES BETWEEN CARDINAL INVARIANTS
Authors: FISCHER VERA , SOUKUP DÁNIEL T.
Published: August 2021

Motivated by recent results and questions of Raghavan and Shelah, we present ZFC theorems on the bounding and various almost disjointness numbers, as well as on reaping and dominating families on uncountable, regular cardinals. We show that if
$\kappa =\lambda ^+$
for some
$\lambda \geq \omega $
and
$\mathfrak {b}(\kappa )=\kappa ^+$
then
$\mathfrak {a}_e(\kappa )=\mathfrak {a}_p(\kappa )=\kappa ^+$
. If, additionally,
$2^{<\lambda }=\lambda $
then
$\mathfrak {a}_g(\kappa )=\kappa ^+$
as well. Furthermore, we prove a variety of new bounds for
$\mathfrak {d}(\kappa )$
in terms of
$\mathfrak {r}(\kappa )$
, including
$\mathfrak {d}(\kappa )\leq \mathfrak {r}_\sigma (\kappa )\leq \operatorname {\mathrm {cf}}([\mathfrak {r}(\kappa )]^\omega )$
, and
$\mathfrak {d}(\kappa )\leq \mathfrak {r}(\kappa )$
whenever
$\mathfrak {r}(\kappa )<\mathfrak {b}(\kappa )^{+\kappa }$
or
$\operatorname {\mathrm {cf}}(\mathfrak {r}(\kappa ))\leq \kappa $
holds.
Does music affect citizens’ evaluations of candidates?
Authors: Heisbourg Emmanuel , Feitosa Fernando
Published: August 2021

Abstract

While some candidates use music in some of their campaign ads to shape individuals’ perceptions of their competence or compassion, for example, it is unclear whether the relationship between music and trait perceptions is empirically valid. Considering the importance of knowing where trait perceptions—which represent important determinants of the vote—come from and the extent to which it is possible to manipulate trait perceptions by means of music, this study investigates the effect of music on trait perceptions using data from an online survey experiment conducted between October 30 and November 12, 2020. In this experiment, 362 individuals were exposed to a random sequence of five campaign ads, either with their original music or with no music. Following each campaign ad, individuals were asked to evaluate the candidate’s competency, honesty, leadership, and compassion. The analyses reveal that music marginally affects perceptions of competency, honesty, and leadership. Moreover, music exerts no significant effect on perceptions of compassion.
Potential of olive oil and its phenolic compounds as therapeutic intervention against colorectal cancer: a comprehensive review
Authors: Sain Arindam , Sahu Sakshi , Naskar Debdut
Published: August 2021

Colorectal cancer (CRC) is one of the major causes of death across the world and incidence rate of CRC increasing alarmingly each passing year. Diet, genomic anomalies, inflammation and deregulated signalling pathways are among the major causes of CRC. Because of numerous side effects of CRC therapies available now, researchers all over the world looking for alternative treatment/preventive strategy with lesser/no side effects. Olive oil which is part of Mediterranean diet contains numerous phenolic compounds that fight against free radicals and inflammation and also well-known for protective role against CRC. The current review focused on the recent evidences where olive oil and its phenolic compounds such as hydroxytyrosol, oleuropein and oleocanthal showed activities against CRC as well to analyse the cellular and molecular signalling mechanism through which these compounds act on. These compounds shown to combat CRC by reducing proliferation, migration, invasion and angiogenesis through regulation of numerous signalling pathways including MAPK pathway, PI3K-Akt pathway and Wnt/β-catenin pathway and at the same time, induce apoptosis in different CRC model. However, further research is an absolute necessity to establish these compounds as nutritional supplements and develop therapeutic strategy in CRC.
Associations between mental wellbeing and fMRI neural bases underlying responses to positive emotion in a twin sample
Authors: Park Haeme R.P. , Chilver Miranda R. , Montalto Arthur , Jamshidi Javad , Schofield Peter R. , Williams Leanne M. , Gatt Justine M.
Published: August 2021

Background

Although mental wellbeing has been linked with positive health outcomes, including longevity and improved emotional and cognitive functioning, studies examining the underlying neural mechanisms of both subjective and psychological wellbeing have been sparse. We assessed whether both forms of wellbeing are associated with neural activity engaged during positive and negative emotion processing and the extent to which this association is driven by genetics or environment.

Methods

We assessed mental wellbeing in 230 healthy adult monozygotic and dizygotic twins using a previously validated questionnaire (COMPAS-W) and undertook functional magnetic resonance imaging during a facial emotion viewing task. We used linear mixed models to analyse the association between COMPAS-W scores and emotion-elicited neural activation. Univariate twin modelling was used to evaluate heritability of each brain region. Multivariate twin modelling was used to compare twin pairs to assess the contributions of genetic and environmental factors to this association.

Results

Higher levels of wellbeing were associated with greater neural activity in the dorsolateral prefrontal cortex, localised in the right inferior frontal gyrus (IFG), in response to positive emotional expressions of happiness. Univariate twin modelling showed activity in the IFG to have 20% heritability. Multivariate twin modelling suggested that the association between wellbeing and positive emotion-elicited neural activity was driven by common variance from unique environment (r = 0.208) rather than shared genetics.

Conclusions

Higher mental wellbeing may have a basis in greater engagement of prefrontal neural regions in response to positive emotion, and this association may be modifiable by unique life experiences.
Mindfulness-based intervention improves residual negative symptoms and cognitive impairment in schizophrenia: a randomized controlled follow-up study
Authors: Shen Hui , Zhang Li , Li Yuhuan , Zheng Denise , Du Lizhao , Xu Feikang , Xu Chuchen , Liu Yan , Shen Jie , Li Zezhi , Cui Donghong
Published: August 2021

Background

Residual negative symptoms and cognitive impairment are common for chronic schizophrenia patients. The aim of this study was to investigate the efficacy of a mindfulness-based intervention (MBI) on negative and cognitive symptoms of schizophrenia patients with residual negative symptoms.

Methods

In this 6-week, randomized, single-blind, controlled study, a total of 100 schizophrenia patients with residual negative symptoms were randomly assigned to the MBI or control group. The 6-week MBI group and the control group with general rehabilitation programs maintained their original antipsychotic treatments. The scores for the Positive and Negative Syndrome Scale (PANSS), the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS), and the Symptom Checklist 90 (SCL-90) were recorded at baseline and week 6 to assess psychotic symptoms, cognitive performance, and emotional state, respectively.

Results

Compared with general rehabilitation programs, MBI alleviated the PANSS-negative subscore, general psychopathology subscore, and PANSS total score in schizophrenia patients with residual negative symptoms (F = 33.77, pBonferroni < 0.001; F = 42.01, pBonferroni < 0.001; F = 52.41, pBonferroni < 0.001, respectively). Furthermore, MBI improved RBANS total score and immediate memory subscore (F = 8.80, pBonferroni = 0.024; F = 11.37, pBonferroni = 0.006), as well as SCL-90 total score in schizophrenia patients with residual negative symptoms (F = 18.39, pBonferroni < 0.001).

Conclusions

Our results demonstrate that MBI helps schizophrenia patients with residual negative symptoms improve clinical symptoms including negative symptom, general psychopathology symptom, and cognitive impairment.

Trial registration

ChiCTR2100043803.
Asset-rich and cash-poor: which older adults value reverse mortgages?
Authors: Fong Joelle H. , Mitchell Olivia S. , Koh Benedict S. K.
Published: August 2021

Home equity represents a substantial share of retirement wealth for many older persons, particularly in Asia where national housing policies have encouraged home-ownership. This paper explored the potential for reverse mortgages to help ‘asset-rich and cash-poor’ older Singaporeans unlock their home equity while ageing in place. The empirical analysis was based on a nationally representative survey of home-owners age 50+ in the 2018 Singapore Life Panel (N = 6,258). Our analyses showed that the average older home-owner holds some 60 per cent of total net wealth in housing equity, suggestive of high demand potential for reverse mortgage products. Nevertheless, actual interest in such products was much below potential demand. Only one in four older home-owners indicated interest in commercial reverse mortgages if these were to become available; a larger majority had never heard of the financial product. Interest in reverse mortgages was positively associated with product awareness and self-rated product understanding. This implies that a critical step towards building consumer interest would be to enhance awareness of such products and simplify related contract terms. Having a mortgage, fewer children, financial literacy and preparedness for retirement were also positively associated with interest level. These results have implications for targeted interventions to enhance consumer awareness and spur interest in reverse mortgages, especially in ageing societies where older people have built up substantial equity through the housing market over time.
PROSODIC PATTERNS IN SYLHETI-ENGLISH BILINGUALS
Authors: McCarthy Kathleen M. , de Leeuw Esther
Published: August 2021

The primary aim of this study was to investigate prosodic prominence across three groups of Sylheti-English bilinguals: first-generation who arrived as adults, first-generation who arrived as children, and second-generation, i.e., born in the United Kingdom to parents who grew up in Bangladesh. To measure prominence,f0, duration, and intensity were measured across disyllabic words in Sylheti and English. The results showed significant differences in thef0analysis. Regarding monolinguals, Sylheti prominence displayed a rising contour, in contrast to the English falling contour. In Sylheti, the bilinguals born in the United Kingdom were the only group significantly different from the Sylheti monolinguals, displaying an English-like falling pattern in their Sylheti prominence. In English, the late arrival bilinguals displayed a Sylheti-like prominence realization, but the early arrivals and those born in the United Kingdom approximated the monolingual English prominence realization. Overall, language use patterns were the most significant factor related to the bilinguals’ prominence realization.
Single leaflet reconstruction in paediatric aortic regurgitation using the Ozaki procedure
Authors: Shi Tao , Gao Zhan , Li Shoujun , Hua Zhongdong
Published: August 2021

Objectives:

Aortic valve repair in children is still a challenge. The aim of this study was to analyse the surgical results of children with aortic regurgitation who underwent single leaflet reconstruction using the Ozaki procedure in our medical centre.

Methods:

A retrospective study was conducted of nine children with aortic regurgitation who received single leaflet reconstruction from May 2017 to September 2019. Paired t-tests and Wilcoxon signed rank tests were used to compare the data at different time points.

Results:

The median surgical age was 4.7 (3.5, 6.4) years. Eight patients were pre-operatively diagnosed with severe aortic regurgitation, while one had moderate regurgitation. The left ventricles were significantly enlarged, with an average z-score of 3.8. Single leaflet reconstruction was carried out using glutaraldehyde-treated autologous pericardium under the standard Ozaki procedure. The median follow-up was 22 (14, 33) months. There was no post-operative death or re-intervention. One patient had moderate or more aortic regurgitation during the follow-up. The average degree of aortic regurgitation was mild, and the average z-score of the left ventricle decreased to −0.2 in the last follow-up.

Conclusions:

Single leaflet reconstruction using the Ozaki procedure was an effective surgical method for treating children with aortic regurgitation in our centre with satisfactory short-term results.
Association between egg intake and blood pressure in the USA: the INTERnational study on MAcro/micronutrients and blood Pressure (INTERMAP)
Authors: Vu Thanh-Huyen T , Van Horn Linda , Daviglus Martha L , Chan Queenie , Dyer Alan R , Zhong Victor W , Gibson Rachel , Elliott Paul , Stamler Jeremiah
Published: August 2021

Objectives:

To investigate associations of egg intake with blood pressure (BP) and the role of dietary variables and other macro- and micro-nutrients in the association.

Design:

We used cross-sectional data for the USA as part of the INTERnational study on MAcro/micronutrients and blood Pressure (INTERMAP). INTERMAP was surveyed between 1996 and 1999, including four 24-h dietary recalls, two 24-h urine collections and eight measurements of systolic BP and diastolic BP (SBP, DBP). Average egg intake (g/d) was calculated. Multivariable linear regression models were used to estimate the association between egg intake (per each 50 g/d or per quintile) and BP. The roles of dietary variables and other macro- and micro-nutrients in this association were also investigated.

Setting:

In the USA.

Participants:

In total, 2195 US INTERMAP men and women aged 40–59 years.

Results:

Participants were 50 % female, 54 % non-Hispanic White and 16 % non-Hispanic Black. Mean egg intake (sd) in men and women was 30·4(29·8) and 21·6(20·5) g/d, respectively. Adjusting for demographics, socio-economics, lifestyle and urinary Na:K excretion ratios, we found non-linear associations with BP in non-obese women (P-quadratic terms: 0·004 for SBP and 0·035 for DBP).The associations remained after adjusting for dietary variables, macro/micro nutrients or minerals. Dietary cholesterol was highly correlated with egg intake and may factor in the association. No association was found in obese women and in obese or non-obese men.

Conclusion:

Egg intake was non-linearly associated with SBP and DBP in non-obese women, but not in obese women or men. Underlying mechanisms require additional study regarding the role of obesity and sex.
Species and Salvation: Theology of History in the Anthropocene?
Authors: Trüper Henning
Published: August 2021

In this article I will discuss various thoughts of a few recent representatives of the tradition of the philosophy of history—Heinz Dieter Kittsteiner, Ulrich Beck, and finally Karl Rahner—and bring them into a conversation with Dipesh Chakrabarty's work on the problems of human species history and the Anthropocene. The aim of this undertaking is to gain greater clarity on the question of the work that theology continues to do for historical thought. I argue that Rahner's notions about “inclusivism”—according to which the possibility of salvation is vested in the species history of humanity rather than in the history of Christian revelation—and his related notion of an irresolvable tension between “anonymous” and what one might then call “onomastic” histories signal the continuing significance of a theology of the baptismal sacrament for historical thought. Rereading Rahner's thought sheds light on certain quandaries of the Anthropocene discussion, regarding the way in which species history can be related to other kinds of history writing, and the novel opening for theodicy generated by the breakdown of the culture–nature divide.
Symposium on Barry Eidlin’s Labor and the Class Idea in the United States and Canada
Authors: Lichtenstein Nelson , de Leon Cedric , Stepan-Norris Judith , Eidlin Barry
Published: August 2021

Barry Eidlin’s book, Labor and the Class Idea in the United States and Canada (Cambridge University Press, 2018) explains why unions are weaker in the United States than they are in Canada, but have not always been that way. Indeed, unionization rates were virtually identical for much of the twentieth century, then diverged in the 1960s. Against dominant accounts focused on long-standing differences in political cultures and institutions, Eidlin argues that the divergence resulted from different ruling party responses to working class upsurge in both countries during the Great Depression and World War II. In Canada, an initially more hostile state response ended up embedding “the class idea”—the idea of class as a salient, legitimate political category—more deeply in policies, policies, and practices than in the United States, where class interests were reduced to “special interests.” In this symposium, three noted labor scholars engage critically with the book. Cedric de Leon interrogates Eidlin’s account of the role of racial divisions in explaining divergence, noting “more persistence and convergence than there is rupture and divergence” between these two countries on this issue. Nelson Lichtenstein’s critique focuses on the exceptionally vociferous character of US employer hostility, which he argues that Eidlin downplays. And Judith Stepan-Norris notes the surprising lack of actual class actors in a book about class organization, while raising interpretive questions about the relation between labor and the Communist Party in both countries. Eidlin concludes the symposium with a response to the critics.
Where Does Moisture Come From Over the Congo Basin?
Authors: Worden Sarah , Fu Rong , Chakraborty Sudip , Liu Junjie , Worden John
Published: August 2021

The Congo Basin hosts the world's second largest rainforest and is a major rainfall center. However, the primary sources of moisture needed to maintain this forest, either from evapotranspiration (ET) or advection from the ocean, remain unclear. We use satellite observations of the deuterium content of water vapor (), solar induced fluorescence (SIF), precipitation, and atmospheric reanalysis to examine the relative contribution of ET to moisture in the free troposphere. We find that SIF, an indicator of photosynthesis, covaries with in early rainy seasons, suggesting that ET is an important contributor to atmospheric moisture in both the spring and fall rainy seasons. However, the relative contribution of ET to the free tropospheric moisture varies between the two rainy seasons. Observed relative to a range of observationally constrained, isotopic mixing models representative of water vapor coming from land suggests that of the free tropospheric moisture come from ET in February, and in April, versus in August and in October. Reanalysis indicate that this difference between seasons is due to increased advection of ocean air during the fall season, thus reducing the relative contribution of ET to the Congo Basin in the fall. In addition, ET is the primary atmospheric moisture source in the winter and summer dry seasons, consistent with estimates reported in literature. Our results highlight the importance of ET from the Congo rainforest as an important source of moisture for initiating the rainy seasons.
The Abuse of Popular Performance Metrics in Hydrologic Modeling
Authors: Clark Martyn P. , Vogel Richard M. , Lamontagne Jonathan R. , Mizukami Naoki , Knoben Wouter J. M. , Tang Guoqiang , Gharari Shervan , Freer Jim E. , Whitfield Paul H. , Shook Kevin R. , Papalexiou Simon Michael
Published: August 2021

The goal of this commentary is to critically evaluate the use of popular performance metrics in hydrologic modeling. We focus on the Nash‐Sutcliffe Efficiency (NSE) and the Kling‐Gupta Efficiency (KGE) metrics, which are both widely used in hydrologic research and practice around the world. Our specific objectives are: (a) to provide tools that quantify the sampling uncertainty in popular performance metrics; (b) to quantify sampling uncertainty in popular performance metrics across a large sample of catchments; and (c) to prescribe the further research that is, needed to improve the estimation, interpretation, and use of popular performance metrics in hydrologic modeling. Our large‐sample analysis demonstrates that there is substantial sampling uncertainty in the NSE and KGE estimators. This occurs because the probability distribution of squared errors between model simulations and observations has heavy tails, meaning that performance metrics can be heavily influenced by just a few data points. Our results highlight obvious (yet ignored) abuses of performance metrics that contaminate the conclusions of many hydrologic modeling studies: It is essential to quantify the sampling uncertainty in performance metrics when justifying the use of a model for a specific purpose and when comparing the performance of competing models.
Agricultural Drought Prediction Based on Conditional Distributions of Vine Copulas
Authors: Wu Haijiang , Su Xiaoling , Singh Vijay P. , Feng Kai , Niu Jiping
Published: August 2021

Monitoring and prediction of agricultural drought are paramount to food security at the global and regional scales, particularly under the influence of climate change and anthropogenic activities. Soil moisture is an effective indicator for monitoring and characterizing agricultural drought. Soil moisture (agricultural drought) is mainly affected by precipitation (meteorological drought) and temperature (hot conditions). Owing to the flexibility of vine copulas in handling multidimensional variables by decomposing them into pair copula constructions (PCCs), we propose a novel drought prediction method using three predictors, namely antecedent meteorological drought, previous hot conditions, and persistent agricultural drought, based on the conditional distributions of C‐vine copulas in a four‐dimensional scenario. The proposed model was applied to agricultural drought (characterized by the standardized soil moisture index (SSI)) prediction with 1–2‐months lead time for the summer season (i.e., August at a 6‐months timescale) in China. Taking two severe agricultural drought events that occurred in many regions across China in August of 2006 and 2014 as validation cases, the SSI predictions with 1–2‐months lead time using the conditional C‐vine copulas model were found to be generally consistent with the corresponding historical SSI observations in most parts of China. Performance evaluation using the Nash‐Sutcliffe efficiency (NSE), coefficient of determination (R2), and F1 score (F1S) for different climate regions also indicated that this model provided a reliable prediction of agricultural drought for most areas of China. The outcome of this study can serve as a guidance for drought prediction, early warning, and drought mitigation.
Calibration‐Free Complementary Relationship Estimates Terrestrial Evapotranspiration Globally
Authors: Ma Ning , Szilagyi Jozsef , Zhang Yongqiang
Published: August 2021

While large‐scale terrestrial evapotranspiration (ET) information is essential for our understanding of the Earth's water and energy cycles, substantial differences exist in current global ET products due partly to uncertainties in soil‐ and vegetation‐related parameters and/or precipitation forcing. Here a calibration‐free complementary relationship (CR) model, driven purely by routine meteorological forcing (air and dew‐point temperature, wind speed, and net radiation), mainly from ERA5, was employed to estimate global ET rates during 1982–2016. Modeled ET agrees favorably with (a) monthly eddy‐covariance measurements of 129 global FLUXNET sites, and; (b) water‐balance‐derived ET of 52 basins at the multiyear mean and annual scales. Additional evaluations demonstrate that the CR is very competitive, in comparison with other 12 widely used global ET products. The 35‐years mean global land ET rate from the CR is 500 ± 6 mm yr−1 (72.3 ± 0.9 × 103 km3 yr−1) with more than 70% of the land area exhibiting increasing annual ET rates over the study period. Globally, CR ET significantly increased at a rate of 0.31 mm yr−1 during 1982–2016, suggesting a 2.2% increase in global land ET over last 35 years. Model inter‐comparisons indicate that global annual CR ET values and their trend are close to the median of not only the 12 ET products chosen but also that of 20 CMIP6 models. Since this calibration‐free CR model requires no precipitation (except in sea‐shore deserts for a subsequent ET correction), vegetation or soil data, it could be incorporated into complex hydrological and/or climate models, thereby facilitating large‐scale hydrological and climate simulations.
Neural network modelling reveals changes in directional connectivity between cortical and hypothalamic regions with increased BMI
Authors: Voigt Katharina , Razi Adeel , Harding Ian H. , Andrews Zane B. , Verdejo-Garcia Antonio
Published: August 2021

Background/Objectives

Obesity has been ascribed to corticostriatal regions taking control over homeostatic areas. To test this assumption, we applied an effective connectivity approach to reveal the direction of information flow between brain regions and the valence of connections (excitatory versus inhibitory) as a function of increased BMI and homeostatic state.

Subjects/Methods

Forty-one participants (21 overweight/obese) underwent two resting-state fMRI scans: after overnight fasting (hunger) and following a standardised meal (satiety). We used spectral dynamic causal modelling to unravel hunger and increased BMI-related changes in directed connectivity between cortical, insular, striatal and hypothalamic regions.

Results

During hunger, as compared to satiety, we found increased excitation of the ventromedial prefrontal cortex over the ventral striatum and hypothalamus, suggesting enhanced top-down modulation compensating energy depletion. Increased BMI was associated with increased excitation of the anterior insula over the hypothalamus across the hunger and satiety conditions. The interaction of hunger and increased BMI yielded decreased intra-cortical excitation from the dorso-lateral to the ventromedial prefrontal cortex.

Conclusions

Our findings suggest that excess weight and obesity is associated with persistent top-down excitation of the hypothalamus, regardless of homeostatic state, and hunger-related reductions of dorso-lateral to ventromedial prefrontal inputs. These findings are compatible with eating without hunger and reduced self-regulation views of obesity.
Diurnal variations of brown fat thermogenesis and fat oxidation in humans
Authors: Matsushita Mami , Nirengi Shinsuke , Hibi Masanobu , Wakabayashi Hitoshi , Lee Sang-il , Domichi Masayuki , Sakane Naoki , Saito Masayuki
Published: August 2021

Background/objectives

Disturbed circadian rhythm is associated with an increased risk of obesity and metabolic disorders. Brown adipose tissue (BAT) is a site of nonshivering thermogenesis (NST) and plays a role in regulating whole-body energy expenditure (EE), substrate metabolism, and body fatness. In this study, we examined diurnal variations of NST in healthy humans by focusing on their relation to BAT activity.

Methods

Forty-four healthy men underwent 18F-fluoro-2-deoxy-D-glucose positron emission tomography and were divided into Low-BAT and High-BAT groups. In STUDY 1, EE, diet-induced thermogenesis (DIT), and fat oxidation (FO) were measured using a whole-room indirect calorimeter at 27 °C. In STUDY 2, EE, FO, and skin temperature in the region close to BAT depots (Tscv) and in the control region (Tc) were measured at 27 °C and after 90 min cold exposure at 19 °C in the morning and in the evening.

Results

In STUDY 1, DIT and FO after breakfast was higher in the High-BAT group than in the Low-BAT group (P < 0.05), whereas those after dinner were comparable in the two groups. FO in the High-BAT group was higher after breakfast than after dinner (P < 0.01). In STUDY 2, cold-induced increases in EE (CIT), FO, and Tscv relative to Tc in the morning were higher in the High-BAT group than in the Low-BAT group (P < 0.05), whereas those after dinner were comparable in the two groups. CIT in the High-BAT group tended to be higher in the morning than in the evening (P = 0.056).

Conclusion

BAT-associated NST and FO were evident in the morning, but not in the evening, suggesting that the activity of human BAT is higher in the morning than in the evening, and thus may be involved in the association of an eating habit of breakfast skipping with obesity and related metabolic disorders.
Optimization of the fused deposition modeling-based fabrication process for polylactic acid microneedles
Authors: Wu Libo , Park Jongho , Kamaki Yuto , Kim Beomjoon
Published: August 2021

A microneedle (MN) array is a novel biomedical device adopted in medical applications to pierce through the stratum corneum while targeting the viable epidermis and dermis layers of the skin. Owing to their micron-scale dimensions, MNs can minimize stimulations of the sensory nerve fibers in the dermis layer. For medical applications, such as wound healing, biosensing, and drug delivery, the structure of MNs significantly influences their mechanical properties. Among the various microfabrication methods for MNs, fused deposition modeling (FDM), a commercial 3D printing method, shows potential in terms of the biocompatibility of the printed material (polylactic acid (PLA)) and preprogrammable arbitrary shapes. Owing to the current limitations of FDM printer resolution, conventional micron-scale MN structures cannot be fabricated without a post-fabrication process. Hydrolysis in an alkaline solution is a feasible approach for reducing the size of PLA needles printed via FDM. Moreover, weak bonding between PLA layers during additive manufacturing triggers the detachment of PLA needles before etching to the expected sizes. Furthermore, various parameters for the fabrication of PLA MNs with FDM have yet to be sufficiently optimized. In this study, the thermal parameters of the FDM printing process, including the nozzle and printing stage temperatures, were investigated to bolster the interfacial bonding between PLA layers. Reinforced bonding was demonstrated to address the detachment challenges faced by PLA MNs during the chemical etching process. Furthermore, chemical etching parameters, including the etchant concentration, environmental temperature, and stirring speed of the etchant, were studied to determine the optimal etching ratio. To develop a universal methodology for the batch fabrication of biodegradable MNs, this study is expected to optimize the conditions of the FDM-based fabrication process. Additive manufacturing was employed to produce MNs with preprogrammed structures. Inclined MNs were successfully fabricated by FDM printing with chemical etching. This geometrical structure can be adopted to enhance adhesion to the skin layer. Our study provides a useful method for fabricating MN structures for various biomedical applications.
Petrobactin, a siderophore produced by Alteromonas, mediates community iron acquisition in the global ocean
Authors: Manck Lauren E , Park Jiwoon , Tully Benjamin J , Poire Alfonso M , Bundy Randelle M , Dupont Christopher L , Barbeau Katherine A
Published: August 2021

It is now widely accepted that siderophores play a role in marine iron biogeochemical cycling. However, the mechanisms by which siderophores affect the availability of iron from specific sources and the resulting significance of these processes on iron biogeochemical cycling as a whole have remained largely untested. In this study, we develop a model system for testing the effects of siderophore production on iron bioavailability using the marine copiotroph Alteromonas macleodii ATCC 27126. Through the generation of the knockout cell line ΔasbB::kmr, which lacks siderophore biosynthetic capabilities, we demonstrate that the production of the siderophore petrobactin enables the acquisition of iron from mineral sources and weaker iron-ligand complexes. Notably, the utilization of lithogenic iron, such as that from atmospheric dust, indicates a significant role for siderophores in the incorporation of new iron into marine systems. We have also detected petrobactin, a photoreactive siderophore, directly from seawater in the mid-latitudes of the North Pacific and have identified the biosynthetic pathway for petrobactin in bacterial metagenome-assembled genomes widely distributed across the global ocean. Together, these results improve our mechanistic understanding of the role of siderophore production in iron biogeochemical cycling in the marine environment wherein iron speciation, bioavailability, and residence time can be directly influenced by microbial activities.
Bacterial controlled mitigation of dysbiosis in a seaweed disease
Authors: Li Jiasui , Majzoub Marwan E , Marzinelli Ezequiel M , Dai Zhicong , Thomas Torsten , Egan Suhelen
Published: August 2021

Disease in the marine environment is predicted to increase with anthropogenic stressors and already affects major habitat-formers, such as corals and seaweeds. Solutions to address this issue are urgently needed. The seaweed Delisea pulchra is prone to a bleaching disease, which is caused by opportunistic pathogens and involves bacterial dysbiosis. Bacteria that can inhibit these pathogens and/or counteract dysbiosis are therefore hypothesised to reduce disease. This study aimed to identify such disease-protective bacteria and investigate their protective action. One strain, Phaeobacter sp. BS52, isolated from healthy D. pulchra, was antagonistic towards bleaching pathogens and significantly increased the proportion of healthy individuals when applied before the pathogen challenge (pathogen-only vs. BS52 + pathogen: 41–80%), and to a level similar to the control. However, no significant negative correlations between the relative abundances of pathogens and BS52 on D. pulchra were detected. Instead, inoculation of BS52 mitigated pathogen-induced changes in the epibacterial community. These observations suggest that the protective activity of BS52 was due to its ability to prevent dysbiosis, rather than direct pathogen inhibition. This study demonstrates the feasibility of manipulating bacterial communities in seaweeds to reduce disease and that mitigation of dysbiosis can have positive health outcomes.
A general approach to explore prokaryotic protein glycosylation reveals the unique surface layer modulation of an anammox bacterium
Authors: Pabst Martin , Grouzdev Denis S , Lawson Christopher E , Kleikamp Hugo B C , de Ram Carol , Louwen Rogier , Lin Yue Mei , Lücker Sebastian , van Loosdrecht Mark C M , Laureni Michele
Published: August 2021

The enormous chemical diversity and strain variability of prokaryotic protein glycosylation makes their large-scale exploration exceptionally challenging. Therefore, despite the universal relevance of protein glycosylation across all domains of life, the understanding of their biological significance and the evolutionary forces shaping oligosaccharide structures remains highly limited. Here, we report on a newly established mass binning glycoproteomics approach that establishes the chemical identity of the carbohydrate components and performs untargeted exploration of prokaryotic oligosaccharides from large-scale proteomics data directly. We demonstrate our approach by exploring an enrichment culture of the globally relevant anaerobic ammonium-oxidizing bacterium Ca. Kuenenia stuttgartiensis. By doing so we resolve a remarkable array of oligosaccharides, which are produced by two seemingly unrelated biosynthetic routes, and which modify the same surface-layer protein simultaneously. More intriguingly, the investigated strain also accomplished modulation of highly specialized sugars, supposedly in response to its energy metabolism—the anaerobic oxidation of ammonium—which depends on the acquisition of substrates of opposite charges. Ultimately, we provide a systematic approach for the compositional exploration of prokaryotic protein glycosylation, and reveal a remarkable example for the evolution of complex oligosaccharides in bacteria.
PGE2/EP4 skeleton interoception activity reduces vertebral endplate porosity and spinal pain with low-dose celecoxib
Authors: Xue Peng , Wang Shenyu , Lyu Xiao , Wan Mei , Li Xialin , Ma Lei , Ford Neil C. , Li Yukun , Guan Yun , Ding Wenyuan , Cao Xu
Published: August 2021

Skeletal interoception regulates bone homeostasis through the prostaglandin E2 (PGE2) concentration in bone. Vertebral endplates undergo ossification and become highly porous during intervertebral disc degeneration and aging. We found that the PGE2 concentration was elevated in porous endplates to generate spinal pain. Importantly, treatment with a high-dose cyclooxygenase 2 inhibitor (celecoxib, 80 mg·kg−1 per day) decreased the prostaglandin E2 concentration and attenuated spinal pain in mice with lumbar spine instability. However, this treatment impaired bone formation in porous endplates, and spinal pain recurred after discontinuing the treatment. Interestingly, low-dose celecoxib (20 mg·kg−1 per day, which is equivalent to one-quarter of the clinical maximum dosage) induced a latent inhibition of spinal pain at 3 weeks post-treatment, which persisted even after discontinuing treatment. Furthermore, when the prostaglandin E2 concentration was maintained at the physiological level with low-dose celecoxib, endplate porosity was reduced significantly, which was associated with decreased sensory nerve innervation and spinal pain. These findings suggest that low-dose celecoxib may help to maintain skeletal interoception and decrease vertebral endplate porosity, thereby reducing sensory innervation and spinal pain in mice.
Central and peripheral arterial stiffness responses to uninterrupted prolonged sitting combined with a high-fat meal: a randomized controlled crossover trial
Authors: Fryer Simon , Stone Keeron , Paterson Craig , Brown Meghan , Faulkner James , Lambrick Danielle , Credeur Daniel , Zieff Gabriel , Martínez Aguirre-Betolaza Aitor , Stoner Lee
Published: August 2021

Independently, prolonged uninterrupted sitting and the consumption of a meal high in saturated fats acutely disrupt normal cardiovascular function. Currently, the acute effects of these behaviors performed in combination on arterial stiffness, a marker of cardiovascular health, are unknown. This study sought to determine the effect of consuming a high-fat meal (Δ = 51 g fat) in conjunction with prolonged uninterrupted sitting (180 min) on measures of central and peripheral arterial stiffness. Using a randomized crossover design, 13 young healthy males consumed a high-fat (61 g) or low-fat (10 g) meal before 180 min of uninterrupted sitting. Carotid-femoral (cf) and femoral-ankle (fa) pulse wave velocity (PWV), aortic-femoral stiffness gradient (af-SG), superficial femoral PWV beta (β), and oscillometric pulse wave analysis outcomes were assessed pre and post sitting. cfPWV increased significantly more following the high-fat (mean difference [MD] = 0.59 m·s−1) meal than following the low-fat (MD = 0.2 m·s−1) meal, with no change in faPWV in either condition. The af-SG significantly decreased (worsened) (ηp2 = 0.569) over time in the high- and low-fat conditions (ratio = 0.1 and 0.1, respectively). Superficial femoral PWVβ significantly increased over time in the high- and low-fat conditions (ηp2 = 0.321; 0.8 and 0.4 m·s−1, respectively). Triglycerides increased over time in the high-fat trial only (ηp2 = 0.761). There were no significant changes in blood pressure. Consuming a high-fat meal prior to 180 min of uninterrupted sitting augments markers of cardiovascular disease risk more than consuming a low-fat meal prior to sitting.
Stabilizing the framework of SAPO-34 zeolite toward long-term methanol-to-olefins conversion
Authors: Yang Liu , Wang Chang , Zhang Lina , Dai Weili , Chu Yueying , Xu Jun , Wu Guangjun , Gao Mingbin , Liu Wenjuan , Xu Zhaochao , Wang Pengfei , Guan Naijia , Dyballa Michael , Ye Mao , Deng Feng , Fan Weibin , Li Landong
Published: August 2021

As a commercial MTO catalyst, SAPO-34 zeolite exhibits excellent recyclability probably due to its intrinsic good hydrothermal stability. However, the structural dynamic changes of SAPO-34 catalyst induced by hydrocarbon pool (HP) species and the water formed during the MTO conversion as well as its long-term stability after continuous regenerations are rarely investigated and poorly understood. Herein, the dynamic changes of SAPO-34 framework during the MTO conversion were identified by 1D 27Al, 31P MAS NMR, and 2D 31P-27Al HETCOR NMR spectroscopy. The breakage of T-O-T bonds in SAPO-34 catalyst during long-term continuous regenerations in the MTO conversion could be efficiently suppressed by pre-coking. The combination of catalyst pre-coking and water co-feeding is established to be an efficient strategy to promote the catalytic efficiency and long-term stability of SAPO-34 catalysts in the commercial MTO processes, also sheds light on the development of other high stable zeolite catalyst in the commercial catalysis.
Efficient and selective photocatalytic CH4 conversion to CH3OH with O2 by controlling overoxidation on TiO2
Authors: Feng Ningdong , Lin Huiwen , Song Hui , Yang Longxiao , Tang Daiming , Deng Feng , Ye Jinhua
Published: August 2021

The conversion of photocatalytic methane into methanol in high yield with selectivity remains a huge challenge due to unavoidable overoxidation. Here, the photocatalytic oxidation of CH4 into CH3OH by O2 is carried out on Ag-decorated facet-dominated TiO2. The {001}-dominated TiO2 shows a durable CH3OH yield of 4.8 mmol g−1 h−1 and a selectivity of approximately 80%, which represent much higher values than those reported in recent studies and are better than those obtained for {101}-dominated TiO2. Operando Fourier transform infrared spectroscopy, electron spin resonance, and nuclear magnetic resonance techniques are used to comprehensively clarify the underlying mechanism. The straightforward generation of oxygen vacancies on {001} by photoinduced holes plays a key role in avoiding the formation of •CH3 and •OH, which are the main factors leading to overoxidation and are generally formed on the {101} facet. The generation of oxygen vacancies on {001} results in distinct intermediates and reaction pathways (oxygen vacancy → Ti–O2• → Ti–OO–Ti and Ti–(OO) → Ti–O• pairs), thus achieving high selectivity and yield for CH4 photooxidation into CH3OH.
Quantifying nanodiamonds biodistribution in whole cells with correlative iono-nanoscopy
Authors: Mi Zhaohong , Chen Ce-Belle , Tan Hong Qi , Dou Yanxin , Yang Chengyuan , Turaga Shuvan Prashant , Ren Minqin , Vajandar Saumitra K. , Yuen Gin Hao , Osipowicz Thomas , Watt Frank , Bettiol Andrew A.
Published: August 2021

Correlative imaging and quantification of intracellular nanoparticles with the underlying ultrastructure is crucial for understanding cell-nanoparticle interactions in biological research. However, correlative nanoscale imaging of whole cells still remains a daunting challenge. Here, we report a straightforward nanoscopic approach for whole-cell correlative imaging, by simultaneous ionoluminescence and ultrastructure mapping implemented with a highly focused beam of alpha particles. We demonstrate that fluorescent nanodiamonds exhibit fast, ultrabright and stable emission upon excitation by alpha particles. Thus, by using fluorescent nanodiamonds as imaging probes, our approach enables quantification and correlative localization of single nanodiamonds within a whole cell at sub-30 nm resolution. As an application example, we show that our approach, together with Monte Carlo simulations and radiobiological experiments, can be employed to provide unique insights into the mechanisms of nanodiamond radiosensitization at the single whole-cell level. These findings may benefit clinical studies of radio-enhancement effects by nanoparticles in charged-particle cancer therapy.
SARS-CoV-2 N protein promotes NLRP3 inflammasome activation to induce hyperinflammation
Authors: Pan Pan , Shen Miaomiao , Yu Zhenyang , Ge Weiwei , Chen Keli , Tian Mingfu , Xiao Feng , Wang Zhenwei , Wang Jun , Jia Yaling , Wang Wenbiao , Wan Pin , Zhang Jing , Chen Weijie , Lei Zhiwei , Chen Xin , Luo Zhen , Zhang Qiwei , Xu Meng , Li Geng , Li Yongkui , Wu Jianguo
Published: August 2021

Excessive inflammatory responses induced upon SARS-CoV-2 infection are associated with severe symptoms of COVID-19. Inflammasomes activated in response to SARS-CoV-2 infection are also associated with COVID-19 severity. Here, we show a distinct mechanism by which SARS-CoV-2 N protein promotes NLRP3 inflammasome activation to induce hyperinflammation. N protein facilitates maturation of proinflammatory cytokines and induces proinflammatory responses in cultured cells and mice. Mechanistically, N protein interacts directly with NLRP3 protein, promotes the binding of NLRP3 with ASC, and facilitates NLRP3 inflammasome assembly. More importantly, N protein aggravates lung injury, accelerates death in sepsis and acute inflammation mouse models, and promotes IL-1β and IL-6 activation in mice. Notably, N-induced lung injury and cytokine production are blocked by MCC950 (a specific inhibitor of NLRP3) and Ac-YVAD-cmk (an inhibitor of caspase-1). Therefore, this study reveals a distinct mechanism by which SARS-CoV-2 N protein promotes NLRP3 inflammasome activation and induces excessive inflammatory responses.
Klotho inhibits neuronal senescence in human brain organoids
Authors: Shaker Mohammed R. , Aguado Julio , Chaggar Harman Kaur , Wolvetang Ernst J.
Published: August 2021

Aging is a major risk factor for many neurodegenerative diseases. Klotho (KL) is a glycosylated transmembrane protein that is expressed in the choroid plexus and neurons of the brain. KL exerts potent anti-aging effects on multiple cell types in the body but its role in human brain cells remains largely unclear. Here we show that human cortical neurons, derived from human pluripotent stem cells in 2D cultures or in cortical organoids, develop the typical hallmarks of senescent cells when maintained in vitro for prolonged periods of time, and that moderate upregulation or repression of endogenous KL expression in cortical organoids inhibits and accelerates senescence, respectively. We further demonstrate that KL expression alters the expression of senescence-associated genes including, extracellular matrix genes, and proteoglycans, and can act in a paracrine fashion to inhibit neuronal senescence. In summary, our results establish an important role for KL in the regulation of human neuronal senescence and offer new mechanistic insight into its role in human brain aging.
Possible link between Earth’s rotation rate and oxygenation
Authors: Klatt J. M. , Chennu A. , Arbic B. K. , Biddanda B. A. , Dick G. J.
Published: August 2021

The biotic and abiotic controls on major shifts in atmospheric oxygen and the persistence of low-oxygen periods over a majority of Earth’s history remain under debate. Explanations of Earth’s stepwise pattern of oxygenation have mostly neglected the effect of changing diel illumination dynamics linked to daylength, which has increased through geological time due to Earth’s rotational deceleration caused by tidal friction. Here we used microsensor measurements and dynamic modelling of interfacial solute fluxes in cyanobacterial mats to investigate the effect of changing daylength on Precambrian benthic ecosystems. Simulated increases in daylength across Earth’s historical range boosted the diel benthic oxygen export, even when the gross photosynthetic production remained constant. This fundamental relationship between net productivity and daylength emerges from the interaction of diffusive mass transfer and diel illumination dynamics, and is amplified by metabolic regulation and microbial behaviour. We found that the resultant daylength-driven surplus organic carbon burial could have shaped the increase in atmospheric oxygen that occurred during the Great and Neoproterozoic Oxidation Events. Our suggested mechanism, which links the coinciding increases in daylength and atmospheric oxygen via enhanced net productivity, reveals a possible contribution of planetary mechanics to the evolution of Earth’s biology and geochemistry.