bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Docking and Dynamics of Phytochemicals From Chinese Herbs With SARS-CoV-2 RdRp
Authors: Lu Jingyao , Lu Wenpeng , Jiang Houli , Yang Changshui , Dong Xiaoyun
Published: June 2022

The novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is causing coronavirus disease 2019 (COVID-19) pandemic. Ancient Chinese herbal formulas are effective for diseases caused by viral infection, and their effects on COVID-19 are currently being examined. To directly evaluate the role of Chinese herbs in inhibiting replication of SARS-CoV-2, we investigated how the phytochemicals from Chinese herbs interact with the viral RNA-dependent RNA polymerase (RdRP). Total 1025 compounds were screened, and then 181compounds were selected for molecular docking analysis. Four phytochemicals licorice glycoside E, diisooctyl phthalate, (-)-medicocarpin, and glycyroside showed good binding affinity with RdRp. The best complex licorice glycoside E/RdRp forms 3 hydrogen bonds, 4 hydrophobic interactions, 1 pair of Pi-cation/stacking, and 4 salt bridges. Furthermore, docking complexes licorice glycoside E/RdRp and diisooctyl phthalate/RdRp were optimized by molecular dynamics simulation to obtain the stable conformation. These studies indicate that they are promising as antivirals against SARS-CoV-2.
Do Brand-Affiliated Hotels Have Lower Cash-Flow Risk?
Authors: Liu Peng , O’Neill John W.
Published: June 2022

Hotels are generally perceived as the riskiest type of commercial real estate (CRE) investment because hotel “leases” have relatively high turnover. Existing literature regarding CRE investment risk and return lacks investigation of hotels at the unit level—which is the level of analysis undertaken by existing and prospective hotel investors. Two major types of hotels are branded and independent ones. The purpose of this study is to investigate the variability (risk) of key performance indicators (KPIs) such as occupancy rate, and revenues and profit of branded versus independent hotels. Using a large sample of performance data regarding over 4,000 U.S. hotel properties from 2000 to 2019, we examine the extent to which branding affects the volatility of KPIs. We find that brand-affiliated hotels have lower cash flow risk measured as lower volatilities of KPIs compared with independent ones. Furthermore, the level of volatility reduction of branded hotels is greater for profit than for revenue, and profit may be the most important KPI for hotel investors. The magnitude of volatility reduction also increases as the measurement window length (number of years) increases. We also study the long-term returns of branded versus independent hotels. This study contributes to the understanding regarding the relationships between investment risk of branded versus independent hotels, extends the literature regarding hotel investment, and provides hotel investors and analysts information regarding risk to aid decisions such as developing, purchasing, holding, or disposing of hotel assets.
Multi-system Klebsiella sepsis: A tale of destruction form lung to eye
Authors: Basnayake Oshan , Jayarajah Umesh , Gunawardena Kawmadi , Sivaganesh Sivasuriya
Published: June 2022

Klebsiella pneumoniae, found in the gastrointestinal flora is a causative agent of hospital-acquired infections. Although isolated organ infections are common, reports of multi-system involvement are rare. We report on a susceptible patient presenting with disseminated Klebsiella infection with concurrent multi-organ disease involving the lung, liver, prostate and eye. He recovered after prolonged therapy but suffered from permanent sequalae. Early diagnosis and aggressive therapy is facilitated by awareness and a high degree of suspicion in at-risk patient groups.
Post-cesarean severe sepsis and uterine wound disruption presenting as abdominal wound abscess and peritonitis: A case report
Authors: Choden Namgay , Dorji Namkha , Dem Dago , Lhaden Karma
Published: June 2022

With increasing rate of cesarean section in Bhutan, the complications are expected to rise. Uterine scar dehiscence is one of the immediate complications of cesarean section, which has high morbidity and mortality. Herein, we report a case of uterine wound dehiscence with pelvic abscess following cesarean section, who presented with paralytic ileus and surgical site infection. There should be high degree of suspicion of uterine scar dehiscence with pelvic abscess in cases of paralytic ileus and surgical site infection following cesarean section, which does not improve with medical management. What made this case unusual was presentation of uterine scar dehiscence with abdominal wound sepsis. Prompt diagnosis with appropriate treatment is required to prevent morbidity and possible maternal mortality.
Time-specific associations of wearable sensor-based cardiovascular and behavioral readouts with disease phenotypes in the outpatient setting of the Chronic Renal Insufficiency Cohort
Authors: Lahens Nicholas F. , Rahman Mahboob , Cohen Jordana B. , Cohen Debbie L. , Chen Jing , Weir Matthew R. , Feldman Harold I. , Grant Gregory R. , Townsend Raymond R. , Skarke Carsten , Study Investigators* and the CRIC
Published: June 2022

Patients with chronic kidney disease are at risk of developing cardiovascular disease. To facilitate out-of-clinic evaluation, we piloted wearable device-based analysis of heart rate variability and behavioral readouts in patients with chronic kidney disease from the Chronic Renal Insufficiency Cohort and controls (n  =  49). Time-specific partitioning of heart rate variability readouts confirm higher parasympathetic nervous activity during the night (mean RR at night 14.4  ±  1.9 ms vs. 12.8  ±  2.1 ms during active hours; n  =  47, analysis of variance (ANOVA) q  =  0.001). The α2 long-term fluctuations in the detrended fluctuation analysis, a parameter predictive of cardiovascular mortality, significantly differentiated between diabetic and nondiabetic patients (prominent at night with 0.58  ±  0.2 vs. 0.45  ±  0.12, respectively, adj. p  =  0.004). Both diabetic and nondiabetic chronic kidney disease patients showed loss of rhythmic organization compared to controls, with diabetic chronic kidney disease patients exhibiting deconsolidation of peak phases between their activity and standard deviation of interbeat intervals rhythms (mean phase difference chronic kidney disease 8.3 h, chronic kidney disease/type 2 diabetes mellitus 4 h, controls 6.8 h). This work provides a roadmap toward deriving actionable clinical insights from the data collected by wearable devices outside of highly controlled clinical environments.
Three Approaches to the Inquiry into Teacher Identity: A Narrative Review Enlightened by Habermas's Human Interests
Authors: Rensijing (刘任思静) Liu , Hongbiao (尹弘飚) Yin
Published: June 2022

Purpose

This paper attempts to review and conceptualize how different scholars approach research on teacher identity through the lens of three human interests defined by Jürgen Habermas.

Design/Approach/Methods

This literature review, guided by Habermas's three human interests, illustrates the characteristics of three different approaches to the inquiry into teacher identity.

Findings

This paper summarizes three approaches to researching teacher identity and their characteristics, namely the technical approach, the practical approach, and the critical approach. The implications for future research and teacher development are also discussed.

Originality/Value

By incorporating Habermas's three human interests into teacher identity inquiries, this article offers a theoretical narrative review of the approaches to investigating teacher identity. Strengths and weaknesses of each approach as well as the possibility of a combined application of different approaches provide an original discussion of teacher identity research.
I Cannot Let Go of the Passion: Comparing the Interpersonal and Intrapersonal Conceptualizations of Brand Passion
Authors: Gumparthi Vivek Pani , Ambatipudi Vamsidhar , Narang Amandeep Singh
Published: June 2022

Brand passion is a consumer construct that has garnered a lot of attention in the recent past. The literature on brand passion is divided by two broad conceptualizations, which have resulted in nomological inconsistency. The present research tackles this conceptual disagreement by identifying and contrasting the two broad conceptualizations of brand passion. In this vein, the current study compares the impact of brand passion on consumer well-being, positive word of mouth, and social media following. The study also examines the role of “duration of use” as a moderator on the proposed relationships. A total of 336 responses were analyzed using structural equation modeling, and the findings indicate that interpersonal conceptualization is a better conceptualization. The study also elucidates implications and future research directions.
Islamic Entrepreneurship Perception Among Employees in North Macedonia
Authors: Abduli Selajdin , Zeqiri Jusuf , Ibraimi Sadudin , Zuferi Rasim
Published: June 2022

This article aims to investigate employees’ perceptions about the impact of religion on entrepreneurship activities (EAs), behaviour, and responsibilities among Muslims in North Macedonia. Apart from other factors that affects economic development, individuals seem to consider religion as an important element that shapes entrepreneurship in a community. Therefore, the article examines perceptions of Muslims regarding the relationship between Islamic religion and entrepreneurship and whether Islam is perceived as having an impact on the entrepreneurial behaviour. The research was conducted using a quantitative approach, where the data was collected through a structured questionnaire. A total of 352 responses were collected from employees within businesses in North Macedonia. The data was analysed using Structural Equation Modelling (SEM) with SmartPLS. The results revealed that religion is perceived to have a positive significant impact on Muslims entrepreuneurs in their EAs, behaviour and responsibilities. This study provides knowledge on how Islamic EAs are perceived by different business employees in their entrepreneurial activities from an Islamic perspective in North Macedonia.
Weakness, Numbness and Urinary Incontinence in an 11-Year-Old Female
Authors: Isaac Maximo Larissa Maria , March Amanda , Lam Suet Kam
Published: June 2022

Acute disseminated encephalomyelitis (ADEM) affects the central nervous system (CNS) via a rapid and auto-immune process. It has been associated to viral and bacterial infections, as well as after immunization. Since the beginning of the SARS-CoV-2 (COVID-19) pandemic, reports of COVID 19 infection have been linked to ADEM. We present a case of an 11-year-old female with neurological symptoms during the acute phase of her COVID-19 illness, with MRI changes and positive Myelin Oligodendrocyte Glycoprotein (MOG) antibodies, with clinical presentation consistent of ADEM.
Book review: Md Saidul Islam and Edson Kieu, Climate Change and Food Security in Asia Pacific: Response and Resilience
Authors: Chen Kai
Published: June 2022

Md Saidul Islam and Edson Kieu, Climate Change and Food Security in Asia Pacific: Response and Resilience. Palgrave Macmillan, 2021, 240 pp., (hardback). ISBN: 9783030707521.
Hypoglossal Nerve Stimulator Explantation: A Case Series
Authors: Patel Anuj U. , Yalamanchi Pratyusha , Peddireddy Nithin S. , Hoff Paul T.
Published: June 2022

Hypoglossal nerve stimulation (HGNS) has emerged as a successful surgical treatment strategy for moderate to severe obstructive sleep apnea in patients failing first‐line positive airway pressure therapy. HGNS explantation due to adverse events such as pain and infection is rare and has yet to be well described. Here, our correspondence describes the first case series of patients who have undergone explantation of the Inspire HGNS system. Five patients were identified who underwent HGNS explantation. Three patients underwent explantation due to magnetic resonance imaging (MRI) incompatibility. One patient underwent explantation due to poor cosmesis. One patient underwent explantation due to surgical site infection. Average operative explant time was 163 minutes. MRI incompatibility, poor cosmesis, and device‐related infection are reasons for HGNS explantation. Future need for MRI or chest wall surgery should be considered in patients being evaluated for HGNS implants.
Characterisation of the progression of salts in walls of earthen architecture heritage
Authors: Tavares Alice , Magalhães M. Clara F. , Soares Rosário , Costa Aníbal
Published: June 2022

Two hundred years (1750–1950) of earthen architecture represents an important period of construction in the central region of Portugal. Earthen architecture is usually built close to the coast or to rivers and placed in sandy ground. The impact of rising damp is a general problem and efflorescences are a common cause of damage decay. This problem was studied in a building with two types of earthen construction, adobe masonry walls and formed masonry walls. The aim of this research was to characterise the conditions for the appearance and phase transitions of thénardite and mirabilite, and determine how to prevent progression of salt effects in the two types of wall to support future conservation measures. Laboratory capillarity and porosimetry tests with dolomitic air lime mortar and hygrothermal monitoring were pursued along within situtests. Visual assessment showed that the progression of salts depends on the composition of the earthen materials. To understand these differences, all crystalline solid phases were analysed by powder X-ray diffraction, and building interior hygrothermal conditions were monitored. An investigation into the influence of surface lime water painting and sacrificial mortar application on the crystallisation of sodium sulfates concluded that these also depend on the wall's composition. Data allowed us to conclude that inside the building the temperature and humidity [relative humidity (RH = 100pw/p°w> 70)] conditions led to the adobe breakdown by the fast conversion from thénardite to mirabilite. Therefore, contact with wet atmospheres should be avoided and interior hygrothermal conditions should be controlled. Laboratory andin situtests showed that the environmental conditions of the spaces had effects on the results. The results contribute to understanding of the salt progression and pattern of decay, as well as supporting future recommendations for building conservation, based on the identification of environmental conditions proper to their occurrence.
Enasidenib vs conventional care in older patients with late-stage mutant-IDH2 relapsed/refractory AML: a randomized phase 3 trial
Authors: de Botton Stéphane , Montesinos Pau , Schuh Andre C. , Papayannidis Cristina , Vyas Paresh , Wei Andrew H. , Ommen Hans , Semochkin Sergey , Kim Hee-Je , Larson Richard A. , Koprivnikar Jaime , Frankfurt Olga , Thol Felicitas , Chromik Jörg , Byrne Jenny , Pigneux Arnaud , Thomas Xavier , Salamero Olga , Vidriales Maria Belen , Doronin Vadim , Döhner Hartmut , Fathi Amir T. , Laille Eric , Yu Xin , Hasan Maroof , Martin-Regueira Patricia , DiNardo Courtney D.
Published: June 2022

This open-label, randomized, phase 3 trial (NCT02577406) compared enasidenib, an oral IDH2 (isocitrate dehydrogenase 2) inhibitor, with conventional care regimens (CCRs) in patients aged ≥60 years with late-stage, mutant-IDH2 acute myeloid leukemia (AML) relapsed/refractory (R/R) to 2 or 3 prior AML-directed therapies. Patients were first preselected to a CCR (azacitidine, intermediate-dose cytarabine, low-dose cytarabine, or supportive care) and then randomized (1:1) to enasidenib 100 mg per day or CCR. The primary endpoint was overall survival (OS). Secondary endpoints included event-free survival (EFS), time to treatment failure (TTF), overall response rate (ORR), hematologic improvement (HI), and transfusion independence (TI). Overall, 319 patients were randomized to enasidenib (n = 158) or CCR (n = 161). The median age was 71 years, median (range) enasidenib exposure was 142 days (3 to 1270), and CCR was 36 days (1 to 1166). One enasidenib (0.6%) and 20 CCR (12%) patients received no randomized treatment, and 30% and 43%, respectively, received subsequent AML-directed therapies during follow-up. The median OS with enasidenib vs CCR was 6.5 vs 6.2 months (HR [hazard ratio], 0.86; P = .23); 1-year survival was 37.5% vs 26.1%. Enasidenib meaningfully improved EFS (median, 4.9 vs 2.6 months with CCR; HR, 0.68; P = .008), TTF (median, 4.9 vs 1.9 months; HR, 0.53; P < .001), ORR (40.5% vs 9.9%; P <.001), HI (42.4% vs 11.2%), and red blood cell (RBC)-TI (31.7% vs 9.3%). Enasidenib safety was consistent with prior reports. The primary study endpoint was not met, but OS was confounded by early dropout and subsequent AML-directed therapies. Enasidenib provided meaningful benefits in EFS, TTF, ORR, HI, and RBC-TI in this heavily pretreated older mutant-IDH2 R/R AML population.
A Novel Method for Type Synthesis of Parallel Mechanism Without Parasitic Motion Based on 2R1T Parallel Mechanism with Rotational Bifurcation
Authors: Qin Jinghan , Yu Chunzhan , Sun Zhibo , Cao Lei
Published: June 2022

The parasitic motion has been widely recognized as the major drawback of the parallel mechanism. Therefore a class of 2R1T PMs (parallel mechanism) without parasitic motion has been synthesized. However, these PMs can only rotate around two axes in sequential order. It decreases the performance of the balancing adjustment of the end-effector. In this paper, a family of 2R1T PMs without parasitic motion was reconstructed by using a novel method based on the remarkable properties of rotational bifurcation mechanisms, which can rotate in sequential order. Furthermore, some PMs rotating around two continuous axes in an arbitrary order are established by adding single joints. Taking the practicability of these structures into consideration, the workspace of 3-PRPS PM was analyzed as an example. Moreover, this study explores the practical application of the PMs without parasitic motion in developing balance mechanisms in rough-terrain fire-fighting robots. During the climbing process, the tank is adjusted to be parallel to the horizontal plane in real-time. It is proved that this kind of structure realizes continuous rotation around two rotation axes on the premise of no parasitic motion.
CRISPR/Cas9 gene editing: a new approach for overcoming drug resistance in cancer
Authors: Vaghari-Tabari Mostafa , Hassanpour Parisa , Sadeghsoltani Fatemeh , Malakoti Faezeh , Alemi Forough , Qujeq Durdi , Asemi Zatollah , Yousefi Bahman
Published: June 2022

The CRISPR/Cas9 system is an RNA-based adaptive immune system in bacteria and archaea. Various studies have shown that it is possible to target a wide range of human genes and treat some human diseases, including cancers, by the CRISPR/Cas9 system. In fact, CRISPR/Cas9 gene editing is one of the most efficient genome manipulation techniques. Studies have shown that CRISPR/Cas9 technology, in addition to having the potential to be used as a new therapeutic approach in the treatment of cancers, can also be used to enhance the effectiveness of existing treatments. Undoubtedly, the issue of drug resistance is one of the main obstacles in the treatment of cancers. Cancer cells resist anticancer drugs by a variety of mechanisms, such as enhancing anticancer drugs efflux, enhancing DNA repair, enhancing stemness, and attenuating apoptosis. Mutations in some proteins of different cellular signaling pathways are associated with these events and drug resistance. Recent studies have shown that the CRISPR/Cas9 technique can be used to target important genes involved in these mechanisms, thereby increasing the effectiveness of anticancer drugs. In this review article, studies related to the applications of this technique in overcoming drug resistance in cancer cells will be reviewed. In addition, we will give a brief overview of the limitations of the CRISP/Cas9 gene-editing technique.
A new approach fits multivariate genomic prediction models efficiently
Authors: Xavier Alencar , Habier David
Published: June 2022

Background

Fast, memory-efficient, and reliable algorithms for estimating genomic estimated breeding values (GEBV) for multiple traits and environments are needed to make timely decisions in breeding. Multivariate genomic prediction exploits genetic correlations between traits and environments to increase accuracy of GEBV compared to univariate methods. These genetic correlations are estimated simultaneously with GEBV, because they are specific to year, environment, and management. However, estimating genetic parameters is computationally demanding with restricted maximum likelihood (REML) and Bayesian samplers, and canonical transformations or orthogonalizations cannot be used for unbalanced experimental designs.

Methods

We propose a multivariate randomized Gauss–Seidel algorithm for simultaneous estimation of model effects and genetic parameters. Two previously proposed methods for estimating genetic parameters were combined with a Gauss–Seidel (GS) solver, and were calledTilde-Hat-GS (THGS) andPseudo-Expectation-GS (PEGS). Balanced and unbalanced experimental designs were simulated to compare runtime, bias and accuracy of GEBV, and bias and standard errors of estimates of heritabilities and genetic correlations of THGS, PEGS, and REML. Models with 10 to 400 response variables, 1279 to 42,034 genetic markers, and 5990 to 1.85 million observations were fitted.

Results

Runtime of PEGS and THGS was a fraction of REML. Accuracies of GEBV were slightly lower than those from REML, but higher than those from the univariate approach, hence THGS and PEGS exploited genetic correlations. For 500 to 600 observations per response variable, biases of estimates of genetic parameters of THGS and PEGS were small, but standard errors of estimates of genetic correlations were higher than for REML. Bias and standard errors decreased as sample size increased. For balanced designs, GEBV and estimates of genetic correlations from THGS were unbiased when only an intercept and eigenvectors of genotype scores were fitted.

Conclusions

THGS and PEGS are fast and memory-efficient algorithms for multivariate genomic prediction for balanced and unbalanced experimental designs. They are scalable for increasing numbers of environments and genetic markers. Accuracy of GEBV was comparable to REML. Estimates of genetic parameters had little bias, but their standard errors were larger than for REML. More studies are needed to evaluate the proposed methods for datasets that contain selection.
miRNA-mRNA analysis of sheep adrenal glands reveals the network regulating reproduction
Authors: Chen Yulin , Liu Yufang , Chu Mingxing
Published: June 2022

Background

The adrenal gland participates in the process of sheep reproduction. MicroRNAs (miRNAs), endogenous small noncoding RNAs, regulate gene expression at the posttranscriptional level. However, the miRNA-mRNA network profile of adrenal glands relating to reproduction in sheep is still not well-studied. As sheep with FecBBB genotype show higher lambing number compare with the sheep with FecB++ genotype. This research aims to compare gene expression by small RNA-seq in adrenal tissues at follicular (F) and luteal (L) phases in FecBBB (MM) and FecB++ (ww) sheep. After analysis of gene expression, significant differentially expressed microRNAs (DEMs) and corresponding target genes were identified.

Results

A total of 180 miRNAs were found in this study, of which 19 DEMs were expressed in the four comparison groups (MM_F_A vs. MM_L_A, MM_F_A vs. ww_F_A, MM_L_A vs. ww_L_A, ww_F_A vs. ww_L_A). Subsequently, 354 target genes of 19 DEMs were predicted by integrated analysis. Cluster analysis was performed by K_means_cluster, and the expression patterns of these DEMs were separated into four subclusters. Functional analysis of target genes was performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The results indicated that the target genes were involved mainly in the Notch signaling pathway, signal transduction, cell communication, innate immune response and amino acid metabolism. Specifically, the Notch signaling pathway, biosynthetic process and metabolic process of pyrimidine nucleotide and amino acid metabolism appear to play key regulatory roles in the sheep fertility trait. Furthermore, miRNA-mRNA interaction networks were constructed by differentially expressed genes combined with our previous study of transcriptome data. The results showed that several key genes, including TDRD3, ANAPC7, CCNL2, BRD2 and MUT, were related to the transformation from the follicular phase to the luteal phase. PLAC8L1, NFAT5, DDX24 and MBD1 were related to the high fecundity of small tail Han sheep.

Conclusions

In this study, the miRNA transcriptome profile was identified, and miRNA-mRNA interaction networks were constructed in adrenal gland tissue of small tail Han sheep, the interaction between miR-370-3p and its targets were considered to play a major role in the reproduction regulation process. The results enriched the number of known miRNAs in adrenal glands and provided novel ideas and further information to demonstrate posttranscriptional regulation mechanisms at follicular and luteal phases in different genotypes of small tail Han sheep.
The extensive transgenerational transcriptomic effects of ocean acidification on the olfactory epithelium of a marine fish are associated with a better viral resistance
Authors: Cohen-Rengifo Mishal , Danion Morgane , Gonzalez Anne-Alicia , Bégout Marie-Laure , Cormier Alexandre , Noël Cyril , Cabon Joëlle , Vitré Thomas , Mark Felix C. , Mazurais David
Published: June 2022

Background

Progressive CO2-induced ocean acidification (OA) impacts marine life in ways that are difficult to predict but are likely to become exacerbated over generations. Although marine fishes can balance acid–base homeostasis efficiently, indirect ionic regulation that alter neurosensory systems can result in behavioural abnormalities. In marine invertebrates, OA can also affect immune system function, but whether this is the case in marine fishes is not fully understood. Farmed fish are highly susceptible to disease outbreak, yet strategies for overcoming such threats in the wake of OA are wanting. Here, we exposed two generations of the European sea bass (Dicentrarchus labrax) to end-of-century predicted pH levels (IPCC RCP8.5), with parents (F1) being exposed for four years and their offspring (F2) for 18 months. Our design included a transcriptomic analysis of the olfactory rosette (collected from the F2) and a viral challenge (exposing F2 to betanodavirus) where we assessed survival rates.

Results

We discovered transcriptomic trade-offs in both sensory and immune systems after long-term transgenerational exposure to OA. Specifically, RNA-Seq analysis of the olfactory rosette, the peripheral olfactory organ, from 18-months-old F2 revealed extensive regulation in genes involved in ion transport and neuronal signalling, including GABAergic signalling. We also detected OA-induced up-regulation of genes associated with odour transduction, synaptic plasticity, neuron excitability and wiring and down-regulation of genes involved in energy metabolism. Furthermore, OA-exposure induced up-regulation of genes involved in innate antiviral immunity (pathogen recognition receptors and interferon-stimulated genes) in combination with down-regulation of the protein biosynthetic machinery. Consistently, OA-exposed F2 challenged with betanodavirus, which causes damage to the nervous system of marine fish, had acquired improved resistance.

Conclusion

F2 exposed to long-term transgenerational OA acclimation showed superior viral resistance, though as their metabolic and odour transduction programs were altered, odour-mediated behaviours might be consequently impacted. Although it is difficult to unveil how long-term OA impacts propagated between generations, our results reveal that, across generations, trade-offs in plastic responses is a core feature of the olfactory epithelium transcriptome in OA-exposed F2 offspring, and will have important consequences for how cultured and wild fish interacts with its environment.
Genetic diversity and population structure of Caryopteris mongholica revealed by reduced representation sequencing
Authors: Ji Ruoxuan , Yu Xiao , Ren Tianmeng , Chang Yuan , Li Zheng , Xia Xinli , Yin Weilun , Liu Chao
Published: June 2022

Background

Caryopteris mongholica Bunge is a rare broad-leaved shrub distributed in the desert and arid regions of Mongol and North China. Due to land reclamation, natural habitat deterioration and anthropogenic activities in recent years, the wild resources have sharply reduced. To effectively protect and rationally use it, we investigated the genetic diversity and population structure from 18 populations across the range of C. mongholica in China by reduced representation sequencing technology.

Results

We found the overall average values of observed heterozygosity (Ho), expected heterozygosity (He), and average nucleotide diversity (π) were 0.43, 0.35 and 0.135, respectively. Furthermore, the NM17 population exhibited higher genetic diversity than other populations. The phylogenetic tree, principal component analysis (PCA) and structure analysis showed the sampled individuals clustered into two main groups. The NM03 population, with individuals clustered in both groups, may be a transitional population located between the two groups. In addition, most genetic variation existed within populations (90.97%) compared to that among the populations (9.03%). Interestingly, geographic and environmental distances were almost equally important to the observed genetic differences. Redundancy analysis (RDA) identified optical radiation (OR), minimum temperature (MIT) and mean annual precipitation (MAP) related variables as the most important environment factors influencing genetic variation, and the importance of MIT was also confirmed in the latent factor mixed models (LFMM).

Conclusions

The results of this study facilitate research on the genetic diversity of C. mongholica. These genetic features provided vital information for conserving and sustainably developing the C. mongholica genetic resources.
Anatomy of diagnosis in a clinical encounter: how clinicians discuss uncertainty with patients
Authors: Khazen Maram , Sullivan Erin E. , Ramos Jason , Mirica Maria , Linzer Mark , Schiff Gordon D. , Olson Andrew P. J.
Published: June 2022

Background

Studies consider the clinical encounter as linear, comprising six phases (opening, problem presentation, history-taking, physical examination, diagnosis, treatment and closing). This study utilizes formal conversation analysis to explore patient-physician interactions and understanding diagnostic utterances during these phases.

Methods

This study is a qualitative sub-analysis that explores how the diagnosis process, along with diagnostic uncertainty, are addressed during 28 urgent care visits. We analyzed physicians’ hypothesis-generation process by focusing on: location of diagnostic utterances during the encounter; whether certain/uncertain diagnostic utterances were revised throughout the encounter; and how physicians tested their hypothesis-generation and managed uncertainty. We recruited 7 primary care physicians (PCPs) and their 28 patients from Brigham and Women’s Hospital (BWH) in 3 urgent care settings. Encounters were audiotaped, transcribed, and coded using NVivo12 qualitative data analysis software. Data were analyzed inductively and deductively, using formal content and conversation analysis.

Results

We identified 62 diagnostic communication utterances in 12 different clinical situations. In most (24/28, 86%) encounters, the diagnosis process was initiated before the diagnosis phase (57% during history taking and 64% during physical examination). In 17 encounters (61%), a distinct diagnosis phase was not observed. Findings show that the diagnosis process is nonlinear in two ways. First, nonlinearity was observed when diagnostic utterances occurred throughout the encounter, with the six encounter phases overlapping, integrating elements of one phase with another. Second, nonlinearity was noted with respect to the resolution of diagnostic uncertainty, with physicians acknowledging uncertainty when explaining their diagnostic reasoning, even during brief encounters.

Conclusions

Diagnosis is often more interactive and nonlinear, and expressions of diagnostic assessments can occur at any point during an encounter, allowing more flexible and potentially more patient-centered communication. These findings are relevant for physicians’ training programs and helping clinicians improve their communication skills in managing uncertain diagnoses.
Streptococcal and Staphylococcus aureus prosthetic joint infections: are they really different?
Authors: Kherabi Yousra , Zeller Valérie , Kerroumi Younes , Meyssonnier Vanina , Heym Beate , Lidove Olivier , Marmor Simon
Published: June 2022

Background

Staphylococci and streptococci are the most frequent pathogens isolated from prosthetic joint infections (PJIs). The aim of this study was to analyze the outcome of streptococcal and methicillin-susceptible Staphylococcus aureus (MSSA) PJIs.

Methods

All monomicrobial streptococcal and MSSA PJIs managed in a French Referral Center (2010–2017) were sampled from the prospective PJIs cohort study. The primary outcome of interest was the cumulative reinfection-free survival at a 2-year follow-up.

Results

Two hundred and nine patients with 91 streptococcal and 132 staphylococcal infections were analyzed. Patients with streptococcal PJI were older, and infection was more frequently hematogenous. Reinfection-free survival rates at 2-years after all treatment strategies were higher for patients with streptococcal PJI (91% vs 81%; P = .012), but differed according to the strategy. After exchange arthroplasty, no outcome differences were observed (89% vs 93%; P = .878); after debridement, antibiotics and implant retention (DAIR), the reinfection-free survival rate was higher for patients with streptococcal PJI (87% vs 60%; P = .062). For patients managed with prolonged suppressive antibiotic therapy (SAT) alone, those with streptococcal PJIs had a 100% infection-free survival (100% vs 31%; P < .0001).

Conclusions

Reinfection-free survival after DAIR and SAT was better for patients with streptococcal than those with MSSA PJIs. No difference was observed after prosthesis exchange.
Impact of efforts to prevent maternal deaths due to obstetric hemorrhage on trends in epidemiology and management of severe postpartum hemorrhage in Japan: a nationwide retrospective study
Authors: Ueda Akihiko , Nakakita Baku , Chigusa Yoshitsugu , Mogami Haruta , Ohtera Shosuke , Kato Genta , Mandai Masaki , Kondoh Eiji
Published: June 2022

Background

The Japan Society of Obstetrics and Gynecology and the Japan Association of Obstetricians and Gynecologists have issued the guidelines and recommendations on postpartum hemorrhage since 2010 and have been conducted widespread educational activities from 2012. The aim of this study was to investigate the impact of these efforts by the Societies to prevent maternal deaths due to obstetric hemorrhage on trends in epidemiology and management of severe postpartum hemorrhage in Japan.

Methods

A national retrospective cohort study was conducted using the national database of health insurance claims for the period 2012 and 2018. The subjects were all insured women who received a blood transfusion for postpartum hemorrhage. The primary endpoints of this study were hysterectomy and maternal mortality. The etiology of hemorrhage, treatment facility, type of procedure, and blood transfusion volume were tabulated.

Results

Women with postpartum hemorrhage that underwent transfusion increased from 3.5 to 5.5 per 1000 deliveries between 2012 and 2018. The most common cause of postpartum hemorrhage was atonic hemorrhage. After insurance coverage in 2013, the intrauterine balloon tamponade use increased to 20.3% of postpartum hemorrhages treated with transfusion in 2018, while the proportion of hysterectomy was decreased from 7.6% (2013–2015) to 6.4% (2016–2018) (p < 0.0001). The proportion of postpartum hemorrhage in maternal deaths decreased from 21.1% (2013–2015) to 14.1% (2016–2018) per all maternal deaths cases (p = 0.14). Cases with postpartum hemorrhage managed in large referral hospitals was increased (65.9% in 2012 to 70.4% in 2018) during the study period (p < 0.0001).

Conclusions

The efforts by the Societies to prevent maternal mortality due to obstetric hemorrhage resulted in a significant decrease in the frequency of hysterectomies and a downward trend in maternal mortality due to obstetric hemorrhage.
Tumor and peritumor radiomics analysis based on contrast-enhanced CT for predicting early and late recurrence of hepatocellular carcinoma after liver resection
Authors: Li Nu , Wan Xiaoting , Zhang Hong , Zhang Zitian , Guo Yan , Hong Duo
Published: June 2022

Background

In China, liver resection has been proven to be one of the most important strategies for hepatocellular carcinoma patients, but the recurrence rate is high. This study sought to investigate the prognostic value of pretreatment tumor and peritumor contrast-enhanced CT radiomics features for early and late recurrence of BCLC stage 0-B hepatocellular carcinoma after liver resection.

Methods

This study involved 329 hepatocellular carcinoma patients after liver resection. A radiomics model was built by using Lasso-Cox regression model. Association between radiomics model and recurrence-free survival was explored by using Harrell’s concordance index (C-Index) and receiver operating characteristic (ROC) curves. Then, we combined the radiomics model and clinical factors to establish a nomogram whose calibration and discriminatory ability were revealed.

Results

Ten significant tumor and peritumor features were screened to build the radiomics model whose C-indices were 0.743 [95% CI, 0.707 to 0.778] and 0.69 [95% CI, 0.629 to 0.751] in the training and validation cohorts. Moreover, the discriminative accuracy of the radiomics model improved with peritumor features entry. The C-indices of the combined model were 0.773 [95% CI, 0.739 to 0.806] and 0.727 [95% CI, 0.667 to 0.787] in the training and validation cohorts, outperforming the radiomics model.

Conclusions

The tumor and peritumor contrast-enhanced CT radiomic signature is a quantitative imaging biomarker that could improve the prediction of early and late recurrence after liver resection for hepatocellular carcinoma patients when used in addition to clinical predictors.
Investigation of BRCAness associated miRNA-gene axes in breast cancer: cell-free miR-182-5p as a potential expression signature of BRCAness
Authors: Darbeheshti Farzaneh , Kadkhoda Sepideh , Keshavarz-Fathi Mahsa , Razi Sepideh , Bahramy Afshin , Mansoori Yaser , Rezaei Nima
Published: June 2022

The concept of the ‘BRCAness’ phenotype implies the properties that some sporadic breast cancers (BC) share withBRCA1/2-mutation carriers with hereditary BC. Breast tumors with BRCAness have deficiencies in homologous recombination repair (HRR), likeBRCA1/2-mutation carriers, and consequently could benefit from poly-(ADP)-ribose polymerase (PARP) inhibitors and DNA-damaging chemotherapy. Triple-negative breast cancers (TNBC) show a higher frequency of BRCAness than the other BC subtypes. Therefore, looking for BRCAness-related biomarkers could improve personalized management of TNBC patients. microRNAs (miRNAs) play a pivotal role in onco-transcriptomic profiles of tumor cells besides their suitable features as molecular biomarkers. The current study aims to evaluate the expression level of some critical miRNAs-mRNA axes in HRR pathway in tumors and plasma samples from BC patients. The expression levels of three multi-target miRNAs, including miR-182-5p, miR-146a-5p, and miR-498, as well as six downstream HRR-related protein-coding genes, have been investigated in the breast tumors and paired adjacent normal tissues by Real-time PCR. In the next step, based on the results derived from the previous step, we examined the level of cell-free miR-182-5p in the blood plasma samples from the patients. Our results highlight the difference between TNBC and non-TNBC tumor subgroups regarding the dysregulation of the key miRNA/mRNA axes involved in the HRR pathway. Also, for the first time, we show that the level of cell-free miR-182-5p in plasma samples from BC patients could be a clue for screening BC patients eligible for receiving PARP inhibitors through a personalized manner. Altogether, some sporadic BC patients, especially sporadic TNBC, have epigenetically dysregulated HRR pathway that could be identified and benefit from BRCAness-specific therapeutic agents.
Effectiveness of crisis resolution home treatment for the management of acute psychiatric crises in Southern Switzerland: a natural experiment based on geography
Authors: Soldini Emiliano , Alippi Maddalena , Zufferey Maria Caiata , Lisi Angela , Lucchini Mario , Albanese Emiliano , Colombo Raffaella Ada , Rossa Simona , Bolla Emilio , Mellacqua Zefiro Benedetto , Larghi Giuseppina , Cordasco Severino , Kawohl Wolfram , Crivelli Luca , Traber Rafael
Published: June 2022

Background

Crisis Resolution Home Treatment (CRHT) is an alternative to inpatient treatment for acute psychiatric crises management. However, evidence on CRHT effectiveness is still limited. In the Canton of Ticino (Southern Switzerland), in 2016 the regional public psychiatric hospital replaced one acute ward with a CRHT. The current study was designed within this evaluation setting to assess the effectiveness of CRHT compared to standard inpatient treatment.

Methods

CRHT was offered to patients aged 18 to 65 with an acute psychiatric crisis that would have required hospitalization. We used a natural experiment based on geography, where intervention and control groups were formed according to the place of residence. Primary endpoints were reduction of psychiatric symptoms at discharge measured using the Health of the Nation Outcome Scales, treatment duration in days, and rate and length of readmissions during a two-year follow-up period after discharge. Safety during the treatment period was measured with the number of serious adverse events (suicide/suicide attempts, major self-harm episodes, acute alcohol/drug intoxications, aggressions to caregivers or family members). We used linear, log-linear and logistic regression models with propensity scores for the main analysis.

Results

We enrolled 321 patients; 67 were excluded because the treatment period was too short and 17 because they were transferred before the end of the treatment. Two hundred thirty-seven patients were available for data analysis, 93 in the intervention group and 144 in the control group. No serious adverse event was observed during the treatment period in both groups. Reduction of psychiatric symptoms at discharge (p-value = 0.359), readmission rates (p-value = 0.563) and length of readmissions (p-value = 0.770) during the two-year follow-up period did not differ significantly between the two groups. Treatment duration was significantly higher in the treatment group (+ 29.6% on average, p-value = 0.002).

Conclusions

CRHT was comparable to standard hospitalization in terms of psychiatric symptoms reduction, readmission rates and length of readmissions, but it was also characterized by a longer first treatment period. However, observational evidence following the study indicated that CRHT duration constantly lowered over time since its introduction in 2016 and became comparable to hospitalization, showing therefore to be an effective alternative also in terms of treatment length.

Trial registration

ISRCTN38472626 (17/11/2020, retrospectively registered).
Spatio-temporal evolution and trend prediction of the incidence of Class B notifiable infectious diseases in China: a sample of statistical data from 2007 to 2020
Authors: Wang Ruo-Nan , Zhang Yue-Chi , Yu Bo-Tao , He Yan-Ting , Li Bei , Zhang Yi-Li
Published: June 2022

Background

With the accelerated global integration and the impact of climatic, ecological and social environmental changes, China will continue to face the challenge of the outbreak and spread of emerging infectious diseases and traditional ones. This study aims to explore the spatial and temporal evolutionary characteristics of the incidence of Class B notifiable infectious diseases in China from 2007 to 2020, and to forecast the trend of it as well. Hopefully, it will provide a reference for the formulation of infectious disease prevention and control strategies.

Methods

Data on the incidence rates of Class B notifiable infectious diseases in 31 provinces, municipalities and autonomous regions of China from 2007 to 2020 were collected for the prediction of the spatio-temporal evolution and spatial correlation as well as the incidence of Class B notifiable infectious diseases in China based on global spatial autocorrelation and Autoregressive Integrated Moving Average (ARIMA).

Results

From 2007 to 2020, the national incidence rate of Class B notifiable infectious diseases (from 272.37 per 100,000 in 2007 to 190.35 per 100,000 in 2020) decreases year by year, and the spatial distribution shows an “east-central-west” stepwise increase. From 2007 to 2020, the spatial clustering of the incidence of Class B notifiable infectious diseases is significant and increasing year by year (Moran’s I index values range from 0.189 to 0.332, p < 0.05). The forecasted incidence rates of Class B notifiable infectious diseases nationwide from 2021 to 2024 (205.26/100,000, 199.95/100,000, 194.74/100,000 and 189.62/100,000) as well as the forecasted values for most regions show a downward trend, with only some regions (Guangdong, Hunan, Hainan, Tibet, Guangxi and Guizhou) showing an increasing trend year by year.

Conclusions

The current study found that since there were significant regional disparities in the prevention and control of infectious diseases in China between 2007 and 2020, the reduction of the incidence of Class B notifiable infectious diseases requires the joint efforts of the surrounding provinces. Besides, special attention should be paid to provinces with an increasing trend in the incidence of Class B notifiable infectious diseases to prevent the re-emergence of certain traditional infectious diseases in a particular province or even the whole country, as well as the outbreak and spread of emerging infectious diseases.