bluuebunny/crossref_metadata_2025 huggingface dataset:
Cell and Tissue Nanomechanics: From Early Development to Carcinogenesis
Authors: Shmelev Mikhail E. , Titov Sergei I. , Belousov Andrei S. , Farniev Vladislav M. , Zhmenia Valeriia M. , Lanskikh Daria V. , Penkova Alina O. , Kumeiko Vadim V.
Published: February 2022

Cell and tissue nanomechanics, being inspired by progress in high-resolution physical mapping, has recently burst into biomedical research, discovering not only new characteristics of normal and diseased tissues, but also unveiling previously unknown mechanisms of pathological processes. Some parallels can be drawn between early development and carcinogenesis. Early embryogenesis, up to the blastocyst stage, requires a soft microenvironment and internal mechanical signals induced by the contractility of the cortical actomyosin cytoskeleton, stimulating quick cell divisions. During further development from the blastocyst implantation to placenta formation, decidua stiffness is increased ten-fold when compared to non-pregnant endometrium. Organogenesis is mediated by mechanosignaling inspired by intercellular junction formation with the involvement of mechanotransduction from the extracellular matrix (ECM). Carcinogenesis dramatically changes the mechanical properties of cells and their microenvironment, generally reproducing the structural properties and molecular organization of embryonic tissues, but with a higher stiffness of the ECM and higher cellular softness and fluidity. These changes are associated with the complete rearrangement of the entire tissue skeleton involving the ECM, cytoskeleton, and the nuclear scaffold, all integrated with each other in a joint network. The important changes occur in the cancer stem-cell niche responsible for tumor promotion and metastatic growth. We expect that the promising concept based on the natural selection of cancer cells fixing the most invasive phenotypes and genotypes by reciprocal regulation through ECM-mediated nanomechanical feedback loop can be exploited to create new therapeutic strategies for cancer treatment.
Pathophysiological Response to SARS-CoV-2 Infection Detected by Infrared Spectroscopy Enables Rapid and Robust Saliva Screening for COVID-19
Authors: Kazmer Seth T. , Hartel Gunter , Robinson Harley , Richards Renee S. , Yan Kexin , van Hal Sebastiaan J. , Chan Raymond , Hind Andrew , Bradley David , Zieschang Fabian , Rawle Daniel J. , Le Thuy T. , Reid David W. , Suhrbier Andreas , Hill Michelle M.
Published: February 2022

Fourier transform infrared (FTIR) spectroscopy provides a (bio)chemical snapshot of the sample, and was recently used in proof-of-concept cohort studies for COVID-19 saliva screening. However, the biological basis of the proposed technology has not been established. To investigate underlying pathophysiology, we conducted controlled infection experiments on Vero E6 cells in vitro and K18-hACE2 mice in vivo. Potentially infectious culture supernatant or mouse oral lavage samples were treated with ethanol or 75% (v/v) Trizol for attenuated total reflectance (ATR)-FTIR spectroscopy and proteomics, or RT-PCR, respectively. Controlled infection with UV-inactivated SARS-CoV-2 elicited strong biochemical changes in culture supernatant/oral lavage despite a lack of viral replication, determined by RT-PCR or a cell culture infectious dose 50% assay. Nevertheless, SARS-CoV-2 infection induced additional FTIR signals over UV-inactivated SARS-CoV-2 infection in both cell and mouse models, which correspond to aggregated proteins and RNA. Proteomics of mouse oral lavage revealed increased secretion of kallikreins and immune modulatory proteins. Next, we collected saliva from a cohort of human participants (n = 104) and developed a predictive model for COVID-19 using partial least squares discriminant analysis. While high sensitivity of 93.48% was achieved through leave-one-out cross-validation, COVID-19 patients testing negative on follow-up on the day of saliva sampling using RT-PCR was poorly predicted in this model. Importantly, COVID-19 vaccination did not lead to the misclassification of COVID-19 negatives. Finally, meta-analysis revealed that SARS-CoV-2 induced increases in the amide II band in all arms of this study and in recently published cohort studies, indicative of altered β-sheet structures in secreted proteins. In conclusion, this study reveals a consistent secretory pathophysiological response to SARS-CoV-2, as well as a simple, robust method for COVID-19 saliva screening using ATR-FTIR.
A Review on the Use of Self-Curing Agents and Its Mechanism in High-Performance Cementitious Materials
Authors: Hamzah Norhaliza , Mohd Saman Hamidah , Baghban Mohammad , Mohd Sam Abdul , Faridmehr Iman , Muhd Sidek Muhd , Benjeddou Omrane , Huseien Ghasan
Published: February 2022

Self-cured concrete is a type of cement-based material that has the unique ability to mitigate the loss rate of water and increase the capacity of concrete to retain water compared to conventional concrete. The technique allows a water-filled internal curing agent to be added to the concrete mixture and then slowly releases water during the hydration process. Many researchers have studied the composition of self-curing concrete using different materials such as artificial lightweight aggregate (LWA), porous superfine powders, superabsorbent polymers (SAP), polyethylene glycol (PEG), natural fibers, and artificial normal-weight aggregate (ANWA) as curing agents. Likewise, physical, mechanical, and microstructure properties, including the mechanisms of curing agents toward self-curing cement-based, were discussed. It was suggested that adopting self-curing agents in concrete has a beneficial effect on hydration, improving the mechanical properties, durability, cracking susceptibility behavior, and mitigating autogenous and drying shrinkage. The interfacial transition zone (ITZ) between the curing agent and the cement paste matrix also improved, and the permeability is reduced.
Identifying Transcripts with Tandem Duplications from RNA-Sequencing Data to Predict BRCA1-Type Primary Breast Cancer
Authors: Qu Shuoying , Martens John W. M. , Hollestelle Antoinette , Smid Marcel
Published: February 2022

Patients with cancers that are deficient for homologous recombination repair (HRD) may benefit from PARP inhibitor treatment. Therefore, methods that identify such cancers are crucial. Using whole genome sequencing data, specific genomic scars derived from somatic mutations and genomic rearrangements can identify HRD tumors, with only BRCA1-like HRD cancers profoundly displaying small (<10 kb) tandem duplications (TDs). In this manuscript we describe a method of detecting BRCA1-type HRD in breast cancer (BC) solely from RNA sequencing data by identifying TDs surfacing in transcribed genes. We find that the number of identified TDs (TD-score) is significantly higher in BRCA1-type vs. BRCA2-type BCs, or vs. HR-proficient BCs (p = 2.4 × 10−6 and p = 2.7 × 10−12, respectively). A TD-score ≥2 shows an 88.2% sensitivity (30 out of 34) to detect a BRCA1-type BC, with a specificity of 64.7% (143 out of 221). Pathway enrichment analyses showed genes implicated in cancer to be affected by TDs of which PTEN was found significantly more frequently affected by a TD in BRCA1-type BC. In conclusion, we here describe a novel method to identify TDs in transcripts and classify BRCA1-type BCs with high sensitivity.
Orchidaceae-Derived Anticancer Agents: A Review
Authors: Śliwiński Tomasz , Kowalczyk Tomasz , Sitarek Przemysław , Kolanowska Marta
Published: February 2022

Species of orchids, which belong to the largest family of flowering plants, are commonly used in folk medicine for the treatment of infections and tumors. However, little is known about the actual chemical composition of these plants and their anticancer properties. In this paper, the most recent literature on orchid-derived bioactive substances with anticancer properties is reviewed. For the assessment, previous papers on the anticancer activity of Orchidaceae published since 2015 were considered. The papers were found by exploring electronic databases. According to the available data, many species of orchids contain potential antitumor chemicals. The bioactive substances in a relatively insignificant number of orchids are identified, and most studies are on Asian taxa. Broader research on American and African species and the correct identification of samples included in the experiments are essential for evaluating the usefulness of orchids as a plant family with vast anticancer potential.
Crosstalk between Long Non Coding RNAs, microRNAs and DNA Damage Repair in Prostate Cancer: New Therapeutic Opportunities?
Authors: Orafidiya Folake , Deng Lin , Bevan Charlotte Lynne , Fletcher Claire Emily
Published: February 2022

It is increasingly appreciated that transcripts derived from non-coding parts of the human genome, such as long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), are key regulators of biological processes both in normal physiology and disease. Their dysregulation during tumourigenesis has attracted significant interest in their exploitation as novel cancer therapeutics. Prostate cancer (PCa), as one of the most diagnosed malignancies and a leading cause of cancer-related death in men, continues to pose a major public health problem. In particular, survival of men with metastatic disease is very poor. Defects in DNA damage response (DDR) pathways culminate in genomic instability in PCa, which is associated with aggressive disease and poor patient outcome. Treatment options for metastatic PCa remain limited. Thus, researchers are increasingly targeting ncRNAs and DDR pathways to develop new biomarkers and therapeutics for PCa. Increasing evidence points to a widespread and biologically-relevant regulatory network of interactions between lncRNAs and miRNAs, with implications for major biological and pathological processes. This review summarises the current state of knowledge surrounding the roles of the lncRNA:miRNA interactions in PCa DDR, and their emerging potential as predictive and diagnostic biomarkers. We also discuss their therapeutic promise for the clinical management of PCa.
Treatment of Neuroendocrine Neoplasms with Radiolabeled Peptides—Where Are We Now
Authors: Naik Mitesh , Al-Nahhas Adil , Khan Sairah R.
Published: February 2022

Peptide receptor radionuclide therapy (PRRT) has been one of the most successful and exciting examples of theranostics in nuclear medicine in recent decades and is now firmly embedded in many treatment algorithms for unresectable or metastatic neuroendocrine neoplasms (NENs) worldwide. It is widely considered to be an effective treatment for well- or moderately differentiated neoplasms, which express high levels of somatostatin receptors that can be selectively targeted. This review article outlines the scientific basis of PRRT in treatment of NENs and describes its discovery dating back to the early 1990s. Early treatments utilizing Indium-111, a γ-emitter, showed promise in reduction in tumor size and improvement in biochemistry, but were also met with high radiation doses and myelotoxic and nephrotoxic effects. Subsequently, stable conjugation of DOTA-peptides with β-emitting radionuclides, such as Yttrium-90 and Lutetium-177, served as a breakthrough for PRRT and studies highlighted their potential in eliciting progression-free survival and quality of life benefits. This article will also elaborate on the key trials which paved the way for its approval and will discuss therapeutic considerations, such as patient selection and administration technique, to optimize its use.
Measuring Emissions from a Demonstrator Heavy-Duty Diesel Vehicle under Real-World Conditions—Moving Forward to Euro VII
Authors: Selleri Tommaso , Gioria Roberto , Melas Anastasios D. , Giechaskiel Barouch , Forloni Fabrizio , Mendoza Villafuerte Pablo , Demuynck Joachim , Bosteels Dirk , Wilkes Thomas , Simons Oliver , Recker Patrick , Lilova Velizara , Onishi Yusuke , Steffen Matthias , Grob Benedikt , Perujo Adolfo , Suarez-Bertoa Ricardo
Published: February 2022

The European Union (EU) has introduced since the early 1990s a series of progressively more stringent emission regulations to control air pollution from the transport sector, commonly known as Euro standards. Following this path, more recently, with the European Green Deal, the European Commission has indicated the intention to review the current air pollutant emissions standards. This study investigates the emission performance of an advanced demonstrator vehicle developed to meet the increasingly more stringent air pollution limits required. Emissions of currently regulated and unregulated components including NH3, N2O, and SPN10 (solid particle number), were studied in a very wide range of real-world operative conditions. The performance of two new generation portable instruments for the onboard measurement of N2O and NH3 were also evaluated in comparison with reference laboratory equipment. Similarly, the measurement accuracy of onboard NOx sensors was also compared to laboratory reference. The vehicle presented low emissions of NOx and NH3 and relatively low emissions of N2O, also compared to data currently available in the literature, in a broad range of operative conditions, which however resulted in a large variability in emissions.
Development of Continuous Gas Generation Method for Hydrogen Chloride Using Azeotropic Hydrochloric Acid System
Authors: Aoyagi Reiji , Sekine Yoshika , Kaifuku Yuichiro , Matsunobu Kunitoshi
Published: February 2022

Standard gases are often prepared using high-pressure gas cylinders. However, it is difficult to accurately prepare a known concentration of hydrogen chloride (HCl) gas using this method because HCl is highly corrosive and adsorptive. In this study, a simple method for the continuous generation of HCl gas was developed using a diffusion tube containing hydrochloric acid and a nitrogen carrier gas. The concentration of HCl produced from this system was almost unstable, but constant gas generation was realized for several hours when azeotropic hydrochloric acid (20.6% HCl in water) and a temperature near the azeotropic point (108.5 °C) were used, resulting in the generation of 103.6 ppm (mean, n = 5) of HCl gas with a relative standard deviation (RSD) of 2.34%. In this case, the percentage of HCl present in the entire gas mixture of HCl and water vapor was 22.5%, which is almost equivalent to the HCl content in the azeotropic hydrochloric acid (20.6%). The HCl concentration could also be controlled by changing the flow rate of the carrier gas. This work demonstrates a simple technique based on the diffusion theory that allows for the constant, controllable generation of a known concentration of HCl gas using an azeotropic hydrochloric acid system.
Comparison of Unnoticed Glove Perforations during Minimally Invasive versus Open Surgeries: A Systematic Review and Meta-Analysis
Authors: Anand Sachit , Pogorelić Zenon , Singh Apoorv , Llorente Muñoz Carlos , Krishnan Nellai , Dhua Anjan , Goel Prabudh , Bajpai Minu
Published: February 2022

Objective: Various studies have depicted the incidence of glove perforations during open (OS) and minimally invasive surgeries (MIS). The aim of this meta-analysis was to compare the incidence of macroscopic and microscopic glove perforations during MIS and OS. Methods: The review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Scientific databases (PubMed, Web of Science, Scopus, and EMBASE) were systematically searched for comparative studies depicting the glove perforation rates during MIS and OS. Risk ratios (RR) were calculated for both the outcomes (dichotomous) and the Mantel–Haenszel method was utilized for the estimation of pooled RR. The methodological quality assessment was performed by two independent investigators using the Downs and Black scale. The main outcomes of the study were the proportion of gloves with gross (macroscopic) perforations and the proportion of gloves with microscopic perforations. Results: Four comparative studies including a total of 1428 gloves (435 from the MIS group) were included. Pooling the data demonstrated no difference in the incidence of macroscopic glove perforations among the MIS and OS groups (RR 0.57, 95% CI 0.21 to 1.54, p = 0.27). On the other hand, the incidence of microscopic perforations was significantly higher in the OS group versus the MIS group (RR 0.72, 95% CI 0.55 to 0.95, p = 0.02). However, all the studies had a moderate risk of bias. Conclusions: When compared to OS, the macroscopic glove perforation rate during MIS showed no significant difference. The incidence of microscopic glove perforations was significantly higher during OS as compared to MIS. However, due to the moderate risk of bias of the available comparative studies, the level of evidence of these studies is limited.
Adaptation of the Behavioural Regulation in Active Commuting to School (BR-ACS) Questionnaire in Portuguese Youth
Authors: Marques Adilson , Santos Thiago , Demetriou Yolanda , Schönbach Dorothea M. I. , Peralta Miguel , Lagestad Pål , Martins João , Kleszczewska Dorota , Dzielska Anna , Gouveia Élvio R.
Published: February 2022

This study aimed to translate and adapt the psychometric properties of the Behavioural Regulation in Active Commuting to School (BR-ACS) questionnaire to young Portuguese students. This study had two stages: (1) translation and adaptation of the questionnaire; (2) evaluation of the psychometric properties. A sample of 338 participants (212 female, 126 male) aged 11 to 19 years (Mage = 15.6 ± 2.1) from 31 cities and Madeira island participated in this study. The confirmatory factor analysis suggested an acceptable fit to the data for the first-order and third-order measurement models. The composite reliability values ranged from 0.71 (identified regulation) to 0.90 (integrated regulation), demonstrating internal consistency. The AVE values ranged from 0.40 (amotivation) to 0.69 (integrated regulation), demonstrating an acceptable convergent validity for all constructs. The model estimation had an acceptable fit, with values akin to those of the first-order tested model. Finally, the results of the multigroup analysis for the successive restricted models (CFI < 0.010 and RMSEA < 0.015) point out that the null hypothesis of factor invariance between gender cannot be rejected. The psychometric properties demonstrates the suitability of this questionnaire among Portuguese youths aged 11 to 19. This questionnaire will help understand the motivation aspects that underpin active commuting to school and consequently help to increase physical activity among Portuguese adolescents.
Impacts of Coastal Breezes on the Environment and Human Life: The Case of Mallorca (Western Mediterranean)
Authors: Alomar-Garau Gabriel , Grimalt-Gelabert Miquel
Published: February 2022

The sea breeze is an atmospheric phenomenon that can appear in many coastal areas of the world. On the island of Mallorca, in the center of the Western Mediterranean, the coastal breezes present prototypical characteristics. They are so consistent that their effectiveness allows for easy scientific verification. The sea breeze system is defined in Mallorca by its multi-directional spatial behavior and its unifying nature. As a result of the physical insularity of the land, coastal breezes have multiple orientations and act in opposite directions. This means that they can operate simultaneously on different coasts, and penetrate inland areas, where they typically converge, eliminating slope winds and forming convective chimneys, creating storms. In this article, we characterize and label the coastal breezes of Mallorca, and we verify their influence in the places where this phenomenon appears. A broader understanding of them reveals their remarkable influence on all spheres of life and human settlement: climatic, ecological, biological and geomorphological, but also cultural, territorial, economic and social.
Postoperative Quality Assessment Score Can Select Patients with High Risk for Locoregional Recurrence in Colon Cancer
Authors: Livadaru Cristian , Moscalu Mihaela , Ghitun Florina Adriana , Huluta Alexandra Ramona , Terinte Cristina , Ferariu Dan , Lunca Sorinel , Dimofte Gabriel Mihail
Published: February 2022

Background: Monitoring surgical quality has been shown to reduce locoregional recurrence (LRR). We previously showed that the arterial stump length (ASL) after complete mesocolic excision (CME) is a reproducible quality instrument and correlates with the lymph-node (LN) yield. We hypothesized that generating an LRR prediction score by integrating the ASL would predict the risk of LRR after suboptimal surgery. Methods: 502 patients with curative resections for stage I–III colon cancer were divided in two groups (CME vs. non-CME) and compared in terms of surgical data, ASL-derived parameters, pathological parameters, LRR and LRR-free survival. A prediction score was generated to stratify patients at high risk for LRR. Results: The ASL showed significantly higher values (50.77 mm ± 28.5 mm) with LRR vs. (45.59 mm ± 28.1 mm) without LRR (p < 0.001). Kaplan–Meier survival analysis showed a significant increase in LRR-free survival at 5.58 years when CME was performed (Group A: 81%), in contrast to non-CME surgery (Group B: 67.2%). Conclusions: The prediction score placed 76.6% of patients with LRR in the high-risk category, with a strong predictive value. Patients with long vascular stumps and positive nodes could benefit from second surgery to complete the mesocolic excision.
Identification of MicroRNAs as Potential Blood-Based Biomarkers for Diagnosis and Therapeutic Monitoring of Active Tuberculosis
Authors: Kim Junseong , Park Heechul , Park Sung-Bae , Lee Eun Ju , Je Min-A , Ahn Eunsol , Sim Bora , Lee Jiyoung , Jin Hyunwoo , Lee Kyung Eun , Cho Sang-Nae , Kang Young Ae , Lee Hyejon , Kim Sunghyun , Kim Jungho
Published: February 2022

Early diagnosis increases the treatment success rate for active tuberculosis (ATB) and decreases mortality. MicroRNAs (miRNAs) have been studied as blood-based markers of several infectious diseases. We performed miRNA profiling to identify differentially expressed (DE) miRNAs using whole blood samples from 10 healthy controls (HCs), 15 subjects with latent tuberculosis infection (LTBI), and 12 patients with ATB, and investigated the expression of the top six miRNAs at diagnosis and over the treatment period in addition to performing miRNA-target gene network and gene ontology analyses. miRNA profiling identified 84 DE miRNAs in patients with ATB, including 80 upregulated and four downregulated miRNAs. Receiver operating characteristic curves of the top six miRNAs exhibited excellent distinguishing efficiency with an area under curve (AUC) value > 0.85. Among them, miR-199a-3p and miR-6886-3p can differentiate between ATB and LTBI. Anti-TB treatment restored the levels of miR-199b-3p, miR-199a-3p, miR-16-5p, and miR-374c-5p to HC levels. Furthermore, 108 predicted target genes were related to the regulation of cellular amide metabolism, intrinsic apoptotic signaling, translation, transforming growth factor beta receptor signaling, and cysteine-type endopeptidase activity. The DE miRNAs identified herein are potential biomarkers for diagnosis and therapeutic monitoring in ATB.
MicroRNAs Related to TACE Treatment Response: A Review of the Literature from a Radiological Point of View
Authors: Bozzato Alessandro Marco , Martingano Paola , Pozzi Mucelli Roberta Antea , Cavallaro Marco Francesco Maria , Cesarotto Matteo , Marcello Cristina , Tiribelli Claudio , Pascut Devis , Pizzolato Riccardo , Pozzi Mucelli Fabio , Giuffrè Mauro , Crocè Lory Saveria , Cova Maria Assunta
Published: February 2022

Hepatocellular Carcinoma (HCC) is the sixth most common cancer in the world. Patients with intermediate stage (Barcelona Clinic Liver Cancer, B stage) hepatocellular carcinoma (HCC) have been able to benefit from TACE (transarterial chemoembolization) as a treatment option. MicroRNAs (miRNAs), i.e., a subclass of non-coding RNAs (ncRNAs), participate in post-transcriptional gene regulation processes and miRNA dysfunction has been associated with apoptosis resistance, cellular proliferation, tumor genesis, and progression. Only a few studies have investigated the role of miRNAs as biomarkers predicting TACE treatment response in HCC. Here, we review the studies’ characteristics from a radiological point of view, also correlating data with radiological images chosen from the cases of our institution.
Inclusion of Ultra-Orthodox Students in Higher Education: A Case Study about Women Seminary in the Engineering College of Jerusalem
Authors: Naor Michael , Pinto Gavriel David , Davidov Pini , Benaroch Julie , Kivel Adit
Published: February 2022

This study examines how far the establishment of an ultra-orthodox (Haredi) women-only curriculum and learning environment crafted for their needs, such as supplementary Biblical studies, fosters students’ enrollment and achievement in STEM. The methodology utilizes interviews with administrators accompanied by a Google questionnaire surveying students in order to compare the secular Azrieli College of Engineering with its new ultra-orthodox branch, Tmura Seminary, which aims to promote the acquisition by ultra-orthodox Jewish women of an engineering diploma so that they can find employment in the job market. This objective is significant because the ultra-orthodox community in Israel represents a unique family setting in which the men devote most of their workday to Judaic studies while the women are the breadwinners. The findings of the current study show that addressing the higher education gender gap by purposeful efforts to include ultra-orthodox women is indeed feasible. Still, it remains a challenging endeavor because besides being first-generation students subject to religious constraints, Haredi students are insufficiently prepared in mathematics and English before arriving at the campus, which requires that they participate in an intensive pre-academic preparatory program. The analysis of results showed differences between ultra-orthodox and secular students in their motivation for study, sources of information about suitable education institutions, balancing work and family life priorities, financial need for scholarships, and career path, which is directed toward finding a job in the high-tech industry rather than continuing after graduation to pursue a Master-level degree.
Real Business Applications and Investments in Blockchain Technology
Authors: Lage Oscar , Saiz-Santos María , Zarzuelo José Manuel
Published: February 2022

This paper provides an empirical study to identify the objective of companies that are currently investing in adopting blockchain technologies to improve their processes and services. Unlike other studies based on the theoretical potential application of blockchain technology in different sectors, the main objective of this paper is to analyze real projects and investment of companies in blockchain technology. More than 100 blockchain projects from different sectors were examined with the aim of extracting the perceived applicability and business value of blockchain technology by managers, customers, and partners. We identified the most demanded business value and functional properties in each sector and company size, as well as the relationship between the properties that are demanded together. This article assesses the main functional values attributed to blockchain, highlighting those really appreciated by companies that invest in them and identifying new applications of blockchain technology in different sectors, and generating organizational change. The article reveals that, as expected, significant deviations are already occurring between theoretical applications identified in the literature and those finally adopted by the industry.
Person Localization Model Based on a Fusion of Acoustic and Visual Inputs
Authors: Koren Leon , Stipancic Tomislav , Ricko Andrija , Orsag Luka
Published: February 2022

PLEA is an interactive, biomimetic robotic head with non-verbal communication capabilities. PLEA reasoning is based on a multimodal approach combining video and audio inputs to determine the current emotional state of a person. PLEA expresses emotions using facial expressions generated in real-time, which are projected onto a 3D face surface. In this paper, a more sophisticated computation mechanism is developed and evaluated. The model for audio-visual person separation can locate a talking person in a crowded place by combining input from the ResNet network with input from a hand-crafted algorithm. The first input is used to find human faces in the room, and the second input is used to determine the direction of the sound and to focus attention on a single person. After an information fusion procedure is performed, the face of the person speaking is matched with the corresponding sound direction. As a result of this procedure, the robot could start an interaction with the person based on non-verbal signals. The model was tested and evaluated under laboratory conditions by interaction with users. The results suggest that the methodology can be used efficiently to focus a robot’s attention on a localized person.
An Assessment of the Net Fluid Balance in the Alberta Basin
Authors: Samaroo Mahendra , Chalaturnyk Rick , Dusseault Maurice , Jackson Richard , Buhlmann Arndt , Custers Hans
Published: February 2022

Net fluid balance in the Alberta Basin has been negative over the last 60 years because extensive fluid production has consistently exceeded injection during this period. However, future gigaton-scale carbon sequestration, among other activities, can result in future cumulative fluid injection exceeding extraction (i.e., a positive net fluid balance). The in-situ net fluid balance (i.e., total fluids produced minus total fluids injected) in this basin over the period 1960–2020 shows that a liquids deficit of 4.53 × 109 m3 and a gas deficit of 6.05 × 1012 m3 currently exist. However, fluid deficits are more significant in the upper stratigraphic intervals (located more than 1 km above the Precambrian Basement) than in the stratigraphic intervals located within 1 km of the Precambrian Basement in most geographic regions. This observation indicates that greater sustainable injection capacity for large-scale fluid injection may exist in the upper stratigraphic intervals (located at more than 1 km above the Precambrian Basement), reducing the potential for generating induced seismicity of concern. Additionally, while fluid depletion rates consistently increased over most of the last 60 years in the Alberta Basin, this trend appears to have changed over the past few years. Such analysis of regional net fluid balance and trends may be useful in assessing regional sustainable fluid storage capacity and managing induced seismicity hazards.
The Nutritional Quality Potential of Microgreens, Baby Leaves, and Adult Lettuce: An Underexploited Nutraceutical Source
Authors: Martínez-Ispizua Eva , Calatayud Ángeles , Marsal José Ignacio , Cannata Claudio , Basile Federico , Abdelkhalik Abdelsattar , Soler Salvador , Valcárcel José Vicente , Martínez-Cuenca Mary-Rus
Published: February 2022

Interest in the cultivation of lettuce landraces is increasing because native varieties, as high-quality products, are particularly attractive to consumers. Lettuce is a popular leafy vegetable worldwide, and interest in the consumption of first leaves (microgreens) and seedlings (baby leaves) has grown due to the general belief that young plants offer higher nutritional value. The content of some bioactive compounds and antioxidants (chlorophylls, carotenoids, anthocyanins, ascorbic acid, phenols, antioxidant activity) was monitored in six lettuce landraces and five commercial varieties, and compared across three development stages: microgreen, baby, and adult. Ascorbic acid and phenolic contents were 42% and 79% higher, respectively, in the early stages than in adult lettuces, and red-leaf varieties (CL4 and L11) stood out. This finding agrees with lettuce’s marked antioxidant capacity and correlates with its pigment contents, especially anthocyanins. The nutritional value of adult lettuce is conditioned by its size, shape, and head structure as phytochemical concentrations are regulated by light. The low content of ascorbic acid, phenolics, and anthocyanins in crisphead lettuce (CL5) is a clear example (49, 67%, and 27% lower, respectively, than the adult mean). Our results indicate the wide variability of lettuces’ nutritional characteristics and emphasize that traditional varieties are a helpful source of agricultural biodiversity.
Quality and Shelf-Life Stability of Pork Meat Fillets Packaged in Multilayer Polylactide Films
Authors: Hernández-García Eva , Vargas María , Torres-Giner Sergio
Published: February 2022

In the present study, the effectiveness of a multilayer film of polylactide (PLA), fully bio-based and compostable, was ascertained to develop a novel sustainable packaging solution for the preservation of fresh pork meat. To this end, the multilayer PLA films were first characterized in terms of their thermal characteristics, structure, mechanical performance, permeance to water and aroma vapors and oxygen, and optical properties and, for the first time, compared with two commercial high-barrier multilayer packaging films. Thereafter, the multilayers were thermosealed to package fillets of fresh pork meat and the physicochemical changes, lipid oxidation levels, and microbiological counts were monitored in the food samples during storage under refrigeration conditions. Results showed that the meat fillets packaged in PLA developed a redder color and showed certain indications of dehydration and oxidation, being more noticeably after 11 days of storage, due to the higher water vapor and oxygen permeance values of the biopolymer multilayer. However, the pH changes and bacterial growth in the cold-stored fresh pork meat samples were minimal and very similar in the three tested multilayer films, successfully accomplishing the requirements of the food quality and safety standards at the end of storage.
Should Panelists Refrain from Wearing a Personal Fragrance Prior to Sensory Evaluation? The Effect of Using Perfume on Olfactory Performance
Authors: Beekman Thadeus L. , Luthra Kaushik , Jeesan Shady Afrin , Bowie Rebecca , Seo Han-Seok
Published: February 2022

It is typically recommended that panelists should refrain from wearing personal fragrances, such as perfume or cologne, prior to sensory evaluation. Interestingly, no study has been reported as to whether panelists’ perceptions of test samples could be affected by personal fragrances worn by themselves. The objective of this study was, therefore, to determine the effect of such a personal fragrance on olfactory performance. Nineteen untrained participants were screened, recruited for, and underwent the Sniffin’ Sticks test designed for measuring olfactory performances that included the odor threshold, discrimination, and identification (TDI). The olfactory performance tasks were conducted under three fragrance level conditions: (1) control (no fragrance), (2) just-about-right (JAR), and (3) excessive, with a preliminary study used to identify both the JAR and excessive fragrance levels. The results showed that the odor discrimination, odor threshold, and combined TDI performances were significantly lowered in the two conditions with the perfume fragrance, while the odor identification performance exhibited no significant differences across all three conditions. These findings provide empirical evidence that even low to moderate levels of personal fragrance can significantly reduce individuals’ olfactory capabilities, possibly subsequently altering the perception of test samples during sensory evaluation.
Optimization of the Recovery of Secondary Metabolites from Defatted Brassica carinata Meal and Its Effects on the Extractability and Functional Properties of Proteins
Authors: Nguyen V. P. Thinh , Stewart Jon D. , Allais Florent , Ioannou Irina
Published: February 2022

The sustainable extraction of secondary metabolites from Brassica agro-industrial by-products often involves the use of high concentrations of ethanol, and/or high temperatures, which tends to decrease the efficiency of protein extraction (yield, profile, etc.). To understand the limits of the combination of these two extraction processes, aqueous ethanol extraction of secondary metabolites (e.g., phenolic compounds and glucosinolates) from Brassica carinata defatted meal was optimized using Response Surface Methodology. The validated models predicted that aqueous ethanol extraction of defatted Carinata meal, with a low aqueous EtOH concentration (22% EtOH) at moderate Te (50 °C), enables the efficient recovery of secondary metabolites (sinapine = 9.12 ± 0.05 mg/gDM, sinigrin = 86.54 ± 3.18 µmol/gDM) while maintaining good protein extractability (59.8 ± 2.1%) from successive alkaline extractions. The evaluation of functional properties of the resulting protein isolates revealed that aqueous extraction, under optimized conditions, improves foaming activity while preserving emulsion ability.
A Digital Forensic View of Windows 10 Notifications
Authors: Domingues Patrício , Andrade Luís , Frade Miguel
Published: February 2022

Windows Push Notifications (WPN) is a relevant part of Windows 10 interaction with the user. It is comprised of badges, tiles and toasts. Important and meaningful data can be conveyed by notifications, namely by so-called toasts that can popup with information regarding a new incoming email or a recent message from a social network. In this paper, we analyze the Windows 10 Notification systems from a digital forensic perspective, focusing on the main forensic artifacts conveyed by WPN. We also briefly analyze Windows 11 first release’s WPN system, observing that internal data structures are practically identical to Windows 10. We provide an open source Python 3 command line application to parse and extract data from the Windows Push Notification SQLite3 database, and a Jython module that allows the well-known Autopsy digital forensic software to interact with the application and thus to also parse and process Windows Push Notifications forensic artifacts. From our study, we observe that forensic data provided by WPN are scarce, although they still need to be considered, namely if traditional Windows forensic artifacts are not available. Furthermore, toasts are clearly WPN’s most relevant source of forensic data.
Dkk3/REIC Deficiency Impairs Spermiation, Sperm Fibrous Sheath Integrity and the Sperm Motility of Mice
Authors: Xue Ruizhi , Lin Wenfeng , Fujita Hirofumi , Sun Jingkai , Kinoshita Rie , Ochiai Kazuhiko , Futami Junichiro , Watanabe Masami , Ohuchi Hideyo , Sakaguchi Masakiyo , Tang Zhengyan , Huang Peng , Nasu Yasutomo , Kumon Hiromi
Published: February 2022

The role of Dickkopf-3 (Dkk3)/REIC (The Reduced Expression in Immortalized Cells), a Wnt-signaling inhibitor, in male reproductive physiology remains unknown thus far. To explore the functional details of Dkk3/REIC in the male reproductive process, we studied the Dkk3/REIC knock-out (KO) mouse model. By examining testicular sections and investigating the sperm characteristics (count, vitality and motility) and ultrastructure, we compared the reproductive features between Dkk3/REIC-KO and wild-type (WT) male mice. To further explore the underlying molecular mechanism, we performed RNA sequencing (RNA-seq) analysis of testicular tissues. Our results showed that spermiation failure existed in seminiferous tubules of Dkk3/REIC-KO mice, and sperm from Dkk3/REIC-KO mice exhibited inferior motility (44.09 ± 8.12% vs. 23.26 ± 10.02%, p < 0.01). The Ultrastructure examination revealed defects in the sperm fibrous sheath of KO mice. Although the average count of Dkk3/REIC-KO epididymal sperm was less than that of the wild-types (9.30 ± 0.69 vs. 8.27 ± 0.87, ×106), neither the gap (p > 0.05) nor the difference in the sperm vitality rate (72.83 ± 1.55% vs. 72.50 ± 0.71%, p > 0.05) were statistically significant. The RNA-seq and GO (Gene Oncology) enrichment results indicated that the differential genes were significantly enriched in the GO terms of cytoskeleton function, cAMP signaling and calcium ion binding. Collectively, our research demonstrates that Dkk3/REIC is involved in the process of spermiation, fibrous sheath integrity maintenance and sperm motility of mice.
Transfer RNA-Derived Small RNAs in the Pathogenesis of Parasitic Protozoa
Authors: Peng Ruofan , Santos Herbert J. , Nozaki Tomoyoshi
Published: February 2022

Transfer RNA (tRNA)-derived small RNAs (tsRNAs) are newly identified non-coding small RNAs that have recently attracted attention due to their functional significance in both prokaryotes and eukaryotes. tsRNAs originated from the cleavage of precursor or mature tRNAs by specific nucleases. According to the start and end sites, tsRNAs can be broadly divided into tRNA halves (31–40 nucleotides) and tRNA-derived fragments (tRFs, 14–30 nucleotides). tsRNAs have been reported in multiple organisms to be involved in gene expression regulation, protein synthesis, and signal transduction. As a novel regulator, tsRNAs have also been identified in various protozoan parasites. The conserved biogenesis of tsRNAs in early-branching eukaryotes strongly suggests the universality of this machinery, which requires future research on their shared and potentially disparate biological functions. Here, we reviewed the recent studies of tsRNAs in several representative protozoan parasites including their biogenesis and the roles in parasite biology and intercellular communication. Furthermore, we discussed the remaining questions and potential future works for tsRNAs in this group of organisms.
Association of Genetic Variants in IL6 Gene (rs1800795) with the Concentration of Inflammatory Markers (IL-6, hs-CRP) and Superoxide Dismutase in the Blood of Patients with Acute Pancreatitis—Preliminary Findings
Authors: Ołdakowska Monika , Ściskalska Milena , Kepinska Marta , Marek Grzegorz , Milnerowicz Halina
Published: February 2022

In the course of acute pancreatitis, interleukin-6 plays an important role as a mediator in the inflammatory response. The course of inflammatory disease is associated with intensive oxidative stress, which may activate transcription factors leading to gene-expression changes. Isoenzymes of superoxide dismutase are involved in the defense against free radicals. This study aimed to evaluate changes in IL-6 concentration and the concentration/activity of superoxide dismutase isoenzymes (SOD1, SOD2, and SOD3) in the blood of patients with acute pancreatitis (AP) in terms of rs1800795 polymorphism in the IL6 gene. In the smoking AP patients group with the GC and GG genotypes, the plasma SOD1 concentration was significantly higher (p = 0.0146 and p = 0.0250, respectively) than in patients with CC genotype for SNP rs1800795 in the IL6 gene. An increase in SOD1 concentration in erythrocytes of AP patients with GC genotypes was also demonstrated compared to the individuals from the group with GG genotype (p = 0.0408). Furthermore, a positive correlation between IL-6 and SOD1 concentrations in the plasma of AP patients with GC genotype for SNP rs1800795 was shown. These results indicate that SOD1 may play a protective role against oxidative damage induced by inflammation in the group of AP patients with GC genotype.
Preliminary Characterisation of an Italian Soft Rock with a Block-in-Matrix Fabric
Authors: Napoli Maria Lia , Milan Lorenzo , Barbero Monica , Scavia Claudio
Published: February 2022

Mélanges are heterogeneous geological deposits and represent the most widespread bimrock (block-in-matrix) formations. This paper presents the efforts undertaken to characterise an Italian mélange composed of a clayey-marly matrix enclosing strong calcareous blocks. Due to its low uniaxial compressive strength, this geomaterial can be classified as a soft rock. The weak nature of the marly matrix and its water sensitivity, as well as the presence of rock inclusions, made the collection and preparation of intact specimens extremely complex and time-consuming operations. The difficulties encountered during these phases are described in detail; the various non-conventional procedures considered and developed to overcome these problems are also presented. The potential of the solutions proposed lies in the fact that they can be conveniently applied to other soft rocks with a block-in-matrix internal arrangement, such as the Italian mélange. To characterise the Italian mélange, point load and uniaxial compressive tests were carried out. From the results of these tests, a conversion factor equal to 14 is proposed to correct the point load strength index in order to estimate the uniaxial compressive strength of soft rocks, such as the mélange under study. Moreover, to estimate local strains and the deformability of the geomaterial, the non-destructive digital image correlation technique was applied.
Chemo-Mechanical Couplings at Granular Contact: The Effect of Mineral Dissolution and Precipitation across the Scales
Authors: Bista Hemanta , Panthi Sadrish , Hu Liangbo
Published: February 2022

Strong interactions between mechanical deformation and chemical reactions may play a critical role in the response of geomaterials or geological systems to evolving environmental circumstances that may occur in both natural and engineered processes. The present study focuses on mineral dissolution and precipitation at the intergranular contact whose consequences are often manifested at the macro-scale where the mechanical and transport properties of the geomaterial may be altered. Discrete element modeling is employed to explore two applications involving such mineral transformations. The first example is primarily focused on the chemo-mechanical coupling mechanisms of intergranular contact in the natural process of pressure solution and secondary compression. The effect of the mineral dissolution on the mechanical response at the grain contact is incorporated into the contact model. Discrete element simulations are performed to examine the overall mechanical response of particle assembles subject to mineral dissolution and the results demonstrate the important role of the kinetic rate characteristics of the dissolution process. The second part of the present study revolves around the effect of mineral precipitation in an engineered process known as microbially induced calcite precipitation for potential soil improvement. The kinetics of involved bio-chemical process is incorporated into on the contact model and the simulation results indicate considerable strengthening effect. Overall, the present study demonstrates the feasibility of discrete element approach as a numerical tool to model coupled chemo-mechanical phenomena across the scales.
Common Pregnancy Complaints Can Lead to Motor Vehicle Collisions or Near-Miss Incidents
Authors: Tsuchikawa Sachi , Hanahara Kyoko , Tateoka Yumiko , Hitosugi Masahito
Published: February 2022

Pregnant women commonly report various health complaints during pregnancy, the occurrence of which is believed to cause human error. However, no study has examined the relationship between the occurrence of pregnancy complaints and the risk of motor vehicle collisions (MVCs). This study aimed to clarify the relationship between the frequency and severity of common pregnancy complaints and the occurrence of MVCs or near-miss incidents. We conducted a multicenter cross-sectional survey of 1000 pregnant women in Shiga Prefecture, Japan. The event group experiencing MVCs or near-miss incidents during pregnancy comprised 10.8% of respondents. The frequency of compression of the stomach or abdomen, tension and cramps in the lower abdomen, pelvic pain, irritability, depressed mood, distractedness, and hot flashes was significantly higher in the event group. The results of our multivariate logistic regression analysis revealed that tension and cramps in the lower abdomen, distractedness, and irritability were independent contributory factors to such events, with an odds ratio of 2.414, 1.849, and 1.746, respectively. Educating pregnant women to avoid driving when experiencing these symptoms would improve maternal and fetal safety.