bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigation of Antibacterial Properties of Corrosion-Resistant 316L Steel Alloyed with 0.2 wt.% and 0.5 wt.% Ag
Authors: Kaplan Mikhail A. , Gorbenko Artem D. , Ivannikov Alexander Y. , Kartabaeva Bakhyt B. , Konushkin Sergey V. , Demin Konstantin Y. , Baikin Alexander S. , Sergienko Konstantin V. , Nasakina Elena O. , Bannykh Igor O. , Gorudko Irina V. , Kolmakov Alexey G. , Simakin Alexander V. , Gudkov Sergey V. , Glinushkin Alexey P. , Sevostyanov Mikhail A.
Published: December 2022

The article is devoted to the study of melted ingots, plates rolled from them, and the resulting spherical powder made of corrosion-resistant 316L steel with the addition of 0.2 wt.% and 0.5 wt.% Ag. The study of antibacterial properties, microstructure, and distribution of silver concentrations, as well as qualitative analysis of silver content was carried out. The optimal mode of homogenization annealing of the ingot was 1050 °C for 9 h, which leads to the formation of an austenitic structure. It is shown that the addition of a small amount of silver does not affect the formation of the austenitic structure and silver is distributed evenly throughout the volume of the ingot. The austenitic structure also prevails in the plates after rolling. Silver is distributed evenly throughout the entire volume of the plate. It is noted that the addition of 0.2 wt.% Ag does not affect the strength, elongation, and microhardness of steel, and the addition of 0.5 wt.% Ag does not significantly reduce the strength of steel, however, all samples meet the mechanical characteristics according to the ASTM A240 standard. The qualitative chemical composition of samples made of corrosion-resistant steels was confirmed by X-ray fluorescence analysis methods. By the method of energy-dispersion analysis, the presence of a uniform distribution of silver over the entire volume of the powder particle was determined. The particles have a spherical shape with a minimum number of defects. The study of the antibacterial activity of plates and powder shows the presence of a clear antibacterial effect (bacteria of the genus Xanthomonas campestris, Erwinia carotovora, Pseudomonas marginalis, Clavibacter michiganensis) in samples No. 2 and No. 3 with the addition of 0.2 wt.% and 0.5 wt.% Ag.
Additive Manufacturing of Dense Ti6Al4V Layer via Picosecond Pulse Laser
Authors: Zhu Xiaomeng , Yin Teng , Hu Yuzhou , Li Siyuan , Wu Dong , Xia Zhilin
Published: December 2022

Ultrashort pulse laser shows good potential for heat control improvement in metal additive manufacturing. The challenge of applying ultrashort pulse laser as the heat source is to form a fully melted and dense microstructure. In this study, a picosecond pulse laser is introduced for fabricating single layer Ti6Al4V samples. The results, by examining through X-ray computed tomography (X-CT), scanning electron microscopy (SEM), show that highly dense Ti6Al4V samples were fabricated with optimized process parameters. The analysis of the cross section presents a three-zones structure from top to bottom in the sequence of the fully melted zone, the partially melted zone, and the heat-affected zone. A semi-quantitative study is performed to estimate the thermal efficiency of melted pool formation. The mechanical properties of the samples are tested using nano-indentation, showing an elastic modulus of 89.74 ± 0.74 GPa. The evidence of dense melted pool with good mechanical properties indicates that the picosecond laser can be integrated as the heat source with the current metal additive manufacturing to fabricate parts with accuracy control for the smaller size of thermal filed.
A Multianalytical Approach for the Characterisation of Materials on Selected Artworks by Monogrammist IP
Authors: Šefců Radka , Chlumská Štěpánka , Antušková Václava , Vavřík Daniel , Kumpová Ivana , Pitthard Václav
Published: December 2022

This paper presents an investigation of wooden artworks from the collection of the National Gallery Prague created by Monogrammist IP–one of the top carvers of the Salzburg-Passau region at the beginning of the 16th century. His wood reliefs were examined to gain a better understanding of the historical techniques used in medieval art workshops. The internal structure of the small relief Visitation was analysed using computed tomography. Tomographic reconstruction made it possible to distinguish wood species, observe the internal structure of the artwork in detail, study the technological procedures and identify earlier repairs, additions and damages. Tomographic investigation proved the use of four types of wood on the relief Visitation, most likely pear, lime, unspecified softwood and other different species used for joining dowels. A combination of non-invasive and micro-destructive analytical techniques was employed for the chemical characterisation of the materials in the surface layers of the artworks. Photomicrographs of the surface were taken to provide material for the initial investigation. Non-invasive material research was conducted using a portable X-ray fluorescence analyser and, in selected cases, an external reflection infrared spectrometer. The detailed analyses on the micro-samples was carried out by optical microscopy, micro-Raman spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy coupled with energy dispersive X-ray spectrometry and gas chromatography with mass spectrometry. A glaze layer based on protein with earth pigment was identified on the relief Christ the Saviour from Death.
Quasistatic Analysis of Precast Segmental Concrete-Filled Steel-Tube Bridge Pier with External Arched Energy Dissipation Device
Authors: Wang Chengquan , Zong Yanwei , Zou Yun , Shen Yonggang , Jiang Jiqing , Yin Chongli
Published: December 2022

In order to further promote the application of segment-assembled bridge piers in medium- and high-intensity areas, and to reduce the post-earthquake damage and repair cost of bridge piers, in this paper, a precast segmental concrete-filled steel-tube bridge pier (PSCFSTBP) with an external arched energy dissipation device (AEDD) is proposed. Firstly, the effectiveness of the finite-element analysis software ABAQUS 6.14-4 is proved by the test results of the PSCFSTBP and the corresponding finite-element model analysis results. Secondly, ABAQUS 6.14-4 was used to establish four-segment PSCFSTBP models with four different structural forms (non-energy dissipation device, external arch steel plate, external vertical steel plate, and external AEDD), and the seismic performance of each model was compared and analyzed under reciprocating displacement loading. The results show that compared with the PSCFSTBP with an external AEDD, the lateral bearing capacity of the PSCFSTBP with an external vertical steel plate is increased by about 11.9%, and the initial stiffness is increased by about 2.5%. Compared with the PSCFSTBP with an external arch steel plate, the lateral bearing capacity, initial stiffness, and energy dissipation capacity are increased by 28.8%, 4.6%, and 13 times, respectively. Compared with the PSCFSTBP without an energy dissipation device, its lateral bearing capacity, initial stiffness, and energy dissipation capacity are increased by 39.4%, 10.4%, and 18 times, respectively. The residual displacement of the PSCFSTBP with an external AEDD is kept within 1 mm in the whole displacement loading stage, the offset rate is less than 1%, and the pier damage is controllable, which can realize rapid repair after an earthquake. Finally, the multi-level energy consumption and local replacement of the AEDD are also explored.
Study of 211Bi and 211Pb Recoils Release from 223Ra Labelled TiO2 Nanoparticles
Authors: Kozempel Ján , Sakmár Michal , Janská Tereza , Vlk Martin
Published: December 2022

Nanoparticles of various materials were proposed as carriers of nuclides in targeted alpha particle therapy to at least partially eliminate the nuclear recoil effect causing the unwanted release of radioactive progeny originating in nuclear decay series of so-called in vivo generators. Here, we report on the study of 211Pb and 211Bi recoils release from the 223Ra surface-labelled TiO2 nanoparticles in the concentration range of 0.01–1 mg/mL using two phase separation methods different in their kinetics in order to test the ability of progeny resorption. We have found significant differences between the centrifugation and the dialysis used for labelled NPs separation as well as that the release of 211Pb and 211Bi from the nanoparticles also depends on the NPs dispersion concentration. These findings support our previously proposed recoils-retaining mechanism of the progeny by their resorption on the NPs surface. At the 24 h time-point, the highest overall released progeny fractions were observed using centrifugation (4.0% and 13.5% for 211Pb and 211Bi, respectively) at 0.01 mg/mL TiO2 concentration. The lowest overall released fractions at the 24 h time-point (1.5% and 2.5% for 211Pb and 211Bi respectively) were observed using dialysis at 1 mg/mL TiO2 concentration. Our findings also indicate that the in vitro stability tests of such radionuclide systems designed to retain recoil-progeny may end up with biased results and particular care needs to be given to in vitro stability test experimental setup to mimic in vivo dynamic conditions. On the other hand, controlled and well-defined progeny release may enhance the alpha-emitter radiation therapy of some tumours.
Quasielastic Light Scattering in the Broadband Brillouin Spectra of Relaxor Ferroelectric PbMg1/3Nb2/3O3
Authors: Derets Nikita K. , Fedoseev Alexander I. , Ko Jae-Hyeon , Kojima Seiji , Lushnikov Sergey G.
Published: December 2022

In this paper, the behavior of quasielastic light scattering (QELS) in a PbMg1/3Nb2/3O3 (PMN) crystal under broadband Brillouin light scattering in a temperature range from 750 K to 80 K was studied. It was shown that QELS consists of two components: narrow (0.9 GHz to 11 GHz) and wide (80 GHz to 600 GHz). The dependencies of the intensity, I, of these components on the frequency, ν, are well described by the power law I ~ eνα, with different α, and are determined by the distribution of the relaxation times. The analysis of the Brillouin spectra showed that the behavior of the relaxation time of both the components of QELS with temperature change is well described by the Arrhenius law. Additionally, in the vicinity of the intermediate temperature T* ≈ 380 K, a critical relaxation time behavior for the narrow component of QELS was detected. In the vicinity of the same temperature, a maximum in the integral intensity of both the components of QELS was observed, which is adjacent to another maximum in the region of the Vogel–Fulcher temperature TVF ≈ 250 K corresponding to the transformation of the crystal to a nonergodic state.
Mechanisms of Growth and Hydrogen Permeation of Zirconium Nitride Film on Zirconium Hydride
Authors: Wang Wenke , Yan Guoqing , Ma Zhaohui , Zhang Jiandong , Wang Lijun , Guo Zhancheng
Published: December 2022

Nitride film as a hydrogen permeation barrier on zirconium hydride has seldom been studied. In this work, the zirconium nitride films were prepared on zirconium hydride in an atmosphere of N2 and N2 + H2 at 500~800 °C, with a holding time of 5 h and 20 h, and the mechanisms of film growth and hydrogen permeation were analyzed. The results showed that the film growth was mostly influenced by the temperature, followed by the reaction atmosphere and the holding time. The hydrogen could increase the nitrogen diffusivity during the formation of zirconium nitride films. The in situ nitriding conditions were optimized as 800 °C, N2 + H2 atmosphere, and 5~20 h. The chemical composition of ZrN-based films was mainly comprised of Zr and N, with a minor content of O. In addition, the film exhibited a major phase of ZrN, accompanied by the coexistence of ZrO2, ZrO, ZrN(NH2), and ZrN0.36H0.8, as well as O-H and N-H bonds based on the XPS analysis. The as-prepared ZrN base films in the present study exhibited superior hydrogen permeation resistance to other ZrO2 films previously reported. The hydrogen permeation resistance of the films could be attributed to the following mechanisms, including the chemical capture of hydrogen by the above-mentioned compounds and bonds; the physical barrier of continuous and dense film incurred from the volume effect of different compounds based on Pilling–Bedworth model and the different nitrogen diffusion coefficients at different temperatures.
Optimization of Pin Position and Angle for Z-Pin-Reinforced Foam Core Sandwich Structures
Authors: Kerche Eduardo Fischer , Kairytė Agnė , Członka Sylwia , da Silva Amanda Albertin Xavier , Tonatto Maikson Luiz Passaia , Bresolin Francisco Luiz , Delucis Rafael de Avila , Amico Sandro Campos
Published: December 2022

Sandwich panels (SP) are very promising components for structures as they ally high levels of specific stiffness and strength. Civil, marine and automotive industries are some examples of the sectors that use SPs frequently. This work demonstrates the potential of manufacturing Z-pin-reinforced foam core SPs, using a design strategy that indicated optimal values for both pin position and angle, keeping the same pin diameter as determined in a previous study. A simple search algorithm was applied to optimize each design, ensuring maximum flexural stiffness. Designs using optimal pin position, optimal pin angle and optimal values for both parameters are herein investigated using numerical and experimental approaches. The optimal pin position yielded an increase in flexural stiffness of around 8.0% when compared to the non-optimized design. In this same comparison, the optimal pin angle by itself increased the flexural stiffness by about 63.0%. Besides, the highest increase in the maximum load was found for those composites, molded with optimized levels of pin position and pin angle, which synergistically contributed to this result. All results were demonstrated with numerical and experimental results and there was a good agreement between them.
Crystal and Electronic Structures of New Two Dimensional 3-NH3-PyPbX4 Haloplumbate Materials
Authors: Selivanov Nikita , Kevorkyants Ruslan , Emeline Alexei , Stoumpos Constantinos C.
Published: December 2022

In this study, we explored both the crystal and electronic structures of new synthesized materials 3-NH3-PyPbX4 (X = Br, I). Both compounds are isostructural, and they crystallize in the monoclinic space group P21/c, with four formula units in the unit cell. According to the analysis of their electronic structures, both compounds are direct semiconductors with direct transitions between valence and conduction bands occurring at the k-points A, Z, and at about half of the distance between the k-points D/D1 and D1/E. An inspection of DOS reveals that, in both perovskites, the highest energy VBs are comprised mainly of electronic states of halogen anions, while the lowest states in the conduction band originate from lead orbitals. In addition, there are two flat bands composed of electronic states of carbon and nitrogen originating from the organic subsystems and presumably corresponding to the π* orbitals of 3-NH3-C5H6N cations. Both materials demonstrate a broad luminescence emission. Two mechanisms of the radiative relaxation based on either self-trapped excitons or on charge transfers between inorganic and organic subsystems are discussed.
Evaluation and Improvement of the Current CRCP Pavement Design Method
Authors: Moharekpour Milad , Liu Pengfei , Oeser Markus
Published: December 2022

Continuously reinforced concrete pavement (CRCP) is a representative type of concrete pavement constructed with continuous steel bars without intermediate transverse expansions. With reference to pavement conditions, CRCP is an exceptional type of concrete pavement according to the Highway Pavement Condition Index (HPCI) and International Roughness Index (IRI). The two main design methods for CRCP are AASHTO 86/93 and the Mechanistic–Empirical Pavement Design Guide (MEPDG). Because of limitations of the AASHTO 86/93 design method, the MEPDG method is more reliable. While incorporating the interactions among geometrics, pavement structure layers, material properties, subgrade, traffic, and environmental conditions, and the prediction values according to the MEPDG method, it matched the measured results of crack spacing and crack width. The MEPDG punchout, crack width and spacing, and load transfer efficiency (LTE) models were evaluated, and results were compared with the test sections in three European countries consisting of different construction details, which were investigated and recorded between 2019 and 2021. In this sense, a calculation tool was developed to consider the influence of different parameters in design process. In addition, sensitivity analyses were executed for the development of punchout, considering various input parameters. The track surveying and the evaluation of the results indicated that the design process can be improved with consideration of some criteria such as crack formation time or adjustment of the correlation between crack width and crack spacing. Due to the very important function of erosion and resulting pumping in the deterioration of CRCP, it is advisable to include the influence of the base layer and the influence of different shoulder type and heavy traffic volume or effect of deflection in the calculations.
Description and Genomic Characterization of Oceaniferula flavus sp. nov., a Novel Potential Polysaccharide-Degrading Candidate of the Difficult-to-Cultivate Phylum Verrucomicrobiota Isolated from Seaweed
Authors: Ye Meng-Qi , Jin Chuan-Bo , Liu Xin-Jiang , Tan Xin-Yun , Ye Yu-Qi , Du Zong-Jun
Published: December 2022

A novel strain, isolate 5K15T, which belongs to difficult-to-cultivate phylum Verrucomicrobiota, was recovered from kelp collected from Li Island, Rongcheng, China. The genome sequence of the strain (genome size 3.95 Mbp) showed the presence of four putative biosynthetic gene clusters (BGCs), namely, two terpene biosynthetic gene clusters, one aryl polyene biosynthetic cluster, and one type III PKS cluster. Genomic analysis revealed 79 sulfatase-encoded genes, 24 sulfatase-like hydrolase/transferase-encoded genes, and 25 arylsulfatase-encoded genes, which indicated the great potential of 5K15T to degrade sulfated polysaccharides. Comparative analysis of 16S rRNA gene sequence showed that the novel strain was most closely related to Oceaniferula marina N1E253T (96.4%). On the basis of evidence from a polyphasic study, it is proposed that the strain 5K15T (= KCTC 82748T = MCCC 1H00442T = SDUM 810003T) be classified as Oceaniferula flavus sp. nov. The strain has the ability of carbohydrate transport and metabolism. This ability allows it to survive in carbohydrate-rich materials such as kelp. It has the potential to be used in the marine drug industry using seaweed.
Prevalence of Dengue, Chikungunya and Zika Viruses in Blood Donors in the State of Pará, Northern Brazil: 2018–2020
Authors: Lamarão Leticia Martins , Corrêa Angelita Silva Miranda , de Castro Renata Bezerra Hermes , de Melo Amaral Carlos Eduardo , Monteiro Patricia Danin Jordão , Palmeira Mauricio Koury , Lopes Luane Nascimento , Oliveira Angela Neves , de Lima Maria Salete Maciel , Moreira-Nunes Caroline Aquino , Burbano Rommel Rodríguez
Published: December 2022

Arboviruses have been reported over the years as constant threats to blood transfusion recipients, given the high occurrence of asymptomatic cases and the fact that the presence of viremia precedes the onset of symptoms, making it possible that infected blood from donors act as a source of dissemination. This work aims to identify the prevalence of dengue virus (DENV), Zika virus (ZIKV) and Chikungunya virus (CHIKV) infection in blood donors during epidemic and non-epidemic periods; classify the donor as symptomatic or asymptomatic; and verify the need to include DENV, CHIKV and ZIKV in the nucleic acid test (NAT) platform in northern Brazil. We investigated 36,133 thousand donations in two years of collection in Northern Brazil. One donor was positive for DENV and one for CHIKV (0.002% prevalence). As the prevalence for arboviruses was low in this study, it would not justify the individual screening of samples from donors in a blood bank. Thus, DENV- and CHIKV-positive samples were simulated in different amounts of sample pools, and both were safely detected by molecular biology even in a pool of 14 samples, which would meet the need to include these three viruses in the routine of blood centers in endemic countries such as Brazil.
Short-Term Stability of Electrochemical Properties of Layer-by-Layer Coated Heterogeneous Ion Exchange Membranes
Authors: Sarapulova Veronika , Nevakshenova Ekaterina , Tsygurina Kseniia , Ruleva Valentina , Kirichenko Anna , Kirichenko Ksenia
Published: December 2022

Layer-by-layer adsorption allows the creation of versatile functional coatings for ion exchange membranes, but the stability of the coating and resulting properties of modified membranes in their operation is a frequently asked question. This paper examines the changes in voltammetric curves of layer-by-layer coated cation exchange membranes and pH-metry of desalination chamber with a studied membrane and an auxiliary anion exchange membrane after short-term tests, including over-limiting current modes. The practical operation of the membranes did not affect the voltammetric curves, but enhanced the generation of H+ and OH− ions in a system with polyethylenimine modified membrane in Ca2+ containing solution. It is shown that a distinction between the voltammetric curves of the membranes modified and the different polyamines persists during the operation and that, in the case of polyethylenimine, there is an additional zone of growth of potential drop in voltammetric curves and stronger generation of H+ and OH− ions as indicated by pH-metry.
Dislocation Density of Electron Beam Powder Bed Fusion Ti–6Al–4V Alloys Determined via Time-Of-Flight Neutron Diffraction Line-Profile Analysis
Authors: Yamanaka Kenta , Mori Manami , Onuki Yusuke , Sato Shigeo , Chiba Akihiko
Published: December 2022

Ti–6Al–4V alloys undergo a multiple phase transformation sequence during electron beam powder bed fusion (EB-PBF) additive manufacturing, forming unique dislocation substructures. Thus, determining the dislocation density is crucial for comprehensively understanding the strengthening mechanisms and deformation behavior. This study performed time-of-flight neutron diffraction (TOF-ND) measurements of Ti–6Al–4V alloys prepared via EB-PBF and examined the dislocation density in the as-built and post-processed states using convolutional multiple whole profile (CMWP) fitting. The present TOF-ND/CMWP approach successfully determined the bulk-averaged dislocation density (6.8 × 1013 m−2) in the as-built state for the α-matrix, suggesting a non-negligible contribution of dislocation hardening. The obtained dislocation density values were comparable to those obtained by conventional and synchrotron X-ray diffraction (XRD) measurements, confirming the reliability of the analysis, and indicating that the dislocations in the α-matrix were homogeneously distributed throughout the as-built specimen. However, the negative and positive neutron scattering lengths of Ti and Al, respectively, lowered the diffraction intensity for the Ti–6Al–4V alloys, thereby decreasing the lower limit of the measurable dislocation density and making the analysis difficult.
Monitoring Xenon Capture in a Metal Organic Framework Using Laser-Induced Breakdown Spectroscopy
Authors: Andrews Hunter , Thallapally Praveen , Robinson Alexander
Published: December 2022

Molten salt reactor operation will necessitate circulation of a cover gas to remove certain evolved fission products and maintain an inert atmosphere. The cover gas leaving the reactor core is expected to contain both noble and non-noble gases, aerosols, volatile species, tritium, and radionuclides and their daughters. To remove these radioactive gases, it is necessary to develop a robust off-gas system, along with novel sensors to monitor the gas stream and the treatment system performance. In this study, a metal organic framework (MOF) was engineered for the capture of Xe, a major contributor to the off-gas source term. The engineered MOF column was tested with a laser-induced breakdown spectroscopy (LIBS) sensor for noble gas monitoring. The LIBS sensor was used to monitor breakthrough tests with various Xe, Kr, and Ar mixtures to determine the Xe selectivity of the MOF column. This study offers an initial demonstration of the feasibility of monitoring off-gas treatment systems using a LIBS sensor to aid in the development of new capture systems for molten salt reactors.
An On-Chip Microscale Vacuum Chamber with High Sealing Performance Using Graphene as Lateral Feedthrough
Authors: Yu Panpan , Zhan Fangyuan , Rao Weidong , Zhao Yanqing , Fang Zheng , Tu Zidong , Li Zhiwei , Guo Dengzhu , Wei Xianlong
Published: December 2022

On-chip microscale vacuum chambers with high sealing performance and electrical feedthroughs are highly desired for microscale vacuum electronic devices and other MEMS devices. In this paper, we report an on-chip microscale vacuum chamber which achieves a high sealing performance by using monolayer graphene as lateral electrical feedthrough. A vacuum chamber with the dimensions of π × 2 mm × 2 mm × 0.5 mm is fabricated by anodically bonding a glass chip with a through-hole between two Si chips in a vacuum, after monolayer graphene electrodes have been transferred to the surface of one of the Si chips. Benefiting from the atomic thickness of monolayer graphene, the leak rate of Si–glass bonding interface with a monolayer graphene feedthrough is measured at less than 2 × 10−11 Pa·m3/s. The monolayer graphene feedthrough exhibits a minor resistance increase from 22.5 Ω to 31 Ω after anodic bonding, showing good electrical conductance. The pressure of the vacuum chamber is estimated to be 185 Pa by measuring the breakdown voltage. Such a vacuum is found to maintain for more than 50 days without obvious degradation, implying a high sealing performance with a leak rate of less than 1.02 × 10−16 Pa·m3/s.
Effects of Ambient Temperature on Nanosecond Laser Micro-Drilling of Polydimethylsiloxane (PDMS)
Authors: Lu Ya , Lin Chaoran , Guo Minghui , Rong Youmin , Huang Yu , Wu Congyi
Published: December 2022

In this research, effects of ambient temperature (−100 °C–200 °C) on nanosecond laser micro-drilling of polydimethylsiloxane (PDMS) was investigated by simulation and experiment. A thermo-mechanical coupled model was established, and it was indicated that the top and bottom diameter of the micro-hole decreased with the decrease of the ambient temperature, and the micro-hole taper increased with the decrease of the ambient temperature. The simulation results showed a good agreement with the experiment results in micro-hole geometry; the maximum prediction errors of the top micro-hole diameter, the bottom micro-hole diameter and micro-hole taper were 2.785%, 6.306% and 9.688%, respectively. The diameter of the heat-affected zone decreased with the decrease of the ambient temperature. The circumferential wrinkles were controlled by radial compressive stress. As the ambient temperature increased from 25 °C to 200 °C, the radial compressive stress gradually decreased, which led to the circumferential wrinkles gradually evolving in the radial direction. This work provides a new idea and method based on ambient temperature control for nanosecond laser processing of PDMS, which provides exciting possibilities for a wider range of engineering applications of PDMS.
Controls on Mg/Ca Ratios in Recent Stromatolites: Insights from Fluvial Systems in the Iberian Range (Spain)
Authors: Auqué Luis F. , Osácar M. Cinta , Arenas Concha , Cukrov Neven , Lojen Sonja , Sancho Carlos
Published: December 2022

The utility of the Mg/Ca elemental ratio of calcite ((Mg/Ca)calcite) as a temperature indicator in continental carbonate deposits is a matter of debate due to the different results obtained by diverse authors. In this study, we aimed to test the reliability of the (Mg/Ca)calcite in fluvial carbonates. We selected the recent tufa stromatolite records of four rivers on the Iberian Peninsula for the trace element analysis based on six-monthly sampling. Previous sedimentary and hydrological studies on these fluvial basins provided the information for this work. The water temperature estimates for the stromatolite (Mg/Ca)calcite substantially differed from the measured water temperatures in most of the studied cases. We thus assessed other factors that participate in the control of the Mg partitioning between water and calcite. The correction of the detrital Mg content yielded water temperatures that matched the measured ones in one of the rivers. The (Mg/Ca)water, water discharge and calcite precipitation rates may also occasionally influence the (Mg/Ca)calcite. The six-month behaviour of some of these parameters could interfere with the relationship between the (Mg/Ca)calcite and water temperature. According to these results, and their comparison with other non-marine carbonates, the wide variety of parameters that are involved in the (Mg/Ca)calcite limit it as a geochemical thermometer in continental sedimentary environments.
Mechanical Behavior Analysis of Fully Grouted Ground Anchor in Soft-Hard Alternating Stratum
Authors: Liu Xiujun , Ma Zhanguo
Published: December 2022

Assuming that the ground anchor is connected with the rock–soil of the sidewall by a tangential linear spring, the load transfer model of the fully grouted ground anchor is established by using the spring element method, and the analytical solutions of the displacement, axial force, and shear stress distribution of the ground anchor in the upper and lower parallel strata foundation and sandwich foundation are given, respectively. Corresponding to the above two kinds of alternating strata, the mechanical behavior of the vertical fully grouted ground anchor in the soft–hard alternating stratum is analyzed using the four conditions in Case 1 and the six conditions in Case 2, respectively. Through the case analysis, it can be concluded that the mechanical behavior of the round anchor is greatly affected by the shear modulus of the shallow stratum, and is less affected by the shear modulus of the deep stratum. The depth of the stratum interface and the thickness of the interlayer have some influence on the mechanical behavior of the whole ground anchor but have little influence on the displacement and axial force distribution of the ground anchor. This paper has certain guidance and reference significance for the design of vertical fully grouted ground anchors in the alternating strata.
Ursolic Acid and Solasodine as Potent Anti-Mycobacterial Agents for Combating Paratuberculosis: An Anti-Inflammatory and In Silico Analysis
Authors: Navabharath Manthena , Srivastava Varsha , Gupta Saurabh , Singh Shoor Vir , Ahmad Sayeed
Published: December 2022

Mycobacterium avium subspecies paratuberculosis (MAP) infection in domestic livestock causes persistent diarrhea, weight loss, and death and is also a potential cause of Crohn’s disease (CD) in humans; notably, treatments against MAP are insufficient, costly, and can cause adverse reactions. Hence, plant-derived bioactive constituents have been taken into consideration in this regard. Herein, we present the results of two bioactive constituents (Solasodine and Ursolic acid) that were evaluated for their safety and efficacy against MAP protein (Dephospho-Coenzyme A kinase (DPCK) by utilizing in vitro assays and different tools of in silico biology. The ADME/t-test, the drug-likeness property test, pharmacophore modelling, and PASS prediction have proven that both the constituents have better binding capacities than the available antibiotic drugs used to target protein inhibition pathways. Through our observations, it can be inferred that these two phytochemicals can be adequately used to treat paratuberculosis, thereby combating inflammatory bowel disorders (IBD) of an autoimmune nature.
5-Chloroisoxazoles: A Versatile Starting Material for the Preparation of Amides, Anhydrides, Esters, and Thioesters of 2H-Azirine-2-carboxylic Acids
Authors: Agafonova Anastasiya V. , Novikov Mikhail S. , Khlebnikov Alexander F.
Published: December 2022

Amides, anhydrides, esters, and thioesters of 2H-azirine-2-carboxylic acids were prepared by a rapid procedure at room temperature involving FeCl2-catalyzed isomerization of 5-chloroisoxazoles to 2H-azirine-2-carbonyl chlorides, followed by reaction with N-, O-, or S-nucleophiles mediated by an ortho-substituted pyridine. With readily available chloroisoxazoles and a nucleophile, 2-picoline can be used as an inexpensive base. When a high yield of the acylation product is important, the reagent 2-(trimethylsilyl)pyridine/ethyl chloroformate is more suitable for the acylation with 2H-azirine-2-carbonyl chlorides.
Nanostructures as Photothermal Agents in Tumor Treatment
Authors: Chen Yuqian , Zhou Futing , Wang Chenshuai , Hu Linlin , Guo Pengfei
Published: December 2022

Traditional methods of tumor treatment such as surgical resection, chemotherapy, and radiation therapy have certain limitations, and their treatment effects are not always satisfactory. As a new tumor treatment method, photothermal therapy based on nanostructures has attracted the attention of researchers due to its characteristics of minimally invasive, low side effects, and inhibition of cancer metastasis. In recent years, there has been a variety of inorganic or organic nanostructures used in the field of photothermal tumor treatment, and they have shown great application prospects. In this paper, the advantages and disadvantages of a variety of nanomaterials/nanostructures as photothermal agents (PTAs) for photothermal therapy as well as their research progress are reviewed. For the sake of clarity, the recently reported nanomaterials/nanostructures for photothermal therapy of tumor are classified into five main categories, i.e., carbon nanostructures, noble metal nanostructures, transition metal sulfides, organic polymer, and other nanostructures. In addition, future perspectives or challenges in the related field are discussed.
Vanillin Induces Relaxation in Rat Mesenteric Resistance Arteries by Inhibiting Extracellular Ca2+ Influx
Authors: Choi Sooyeon , Haam Chae Eun , Oh Eun-Yi , Byeon Seonhee , Choi Soo-Kyoung , Lee Young-Ho
Published: December 2022

Vanillin is a phenolic aldehyde, which is found in plant species of the Vanilla genus. Although recent studies have suggested that vanillin has various beneficial properties, the effect of vanillin on blood vessels has not been studied well. In the present study, we investigated whether vanillin has vascular effects in rat mesenteric resistance arteries. To examine the vascular effect of vanillin, we measured the isometric tension of arteries using a multi-wire myograph system. After the arteries were pre-contracted with high K+ (70 mM) or phenylephrine (5 µM), vanillin was administered. Vanillin induced concentration-dependent vasodilation. Endothelial denudation or treatment of eNOS inhibitor (L-NNA, 300 μM) did not affect the vasodilation induced by vanillin. Treatment of K+ channel inhibitor (TEA, 10 mM) or sGC inhibitor (ODQ, 10 μM) or COX-2 inhibitor (indomethacin, 10 μM) did not affect the vanillin-induced vasodilation either. The treatment of vanillin decreased the contractile responses induced by Ca2+ addition. Furthermore, vanillin significantly reduced vascular contraction induced by BAY K 8644 (30 nM). Vanillin induced concentration-dependent vascular relaxation in rat mesenteric resistance arteries, which was endothelium-independent. Inhibition of extracellular Ca2+ influx was involved in vanillin-induced vasodilation. Treatment of vanillin reduced phopsho-MLC20 in vascular smooth muscle cells. These results suggest the possibility of vanillin as a potent vasodilatory molecule.
Modulation Effect on Tubulin Polymerization, Cytotoxicity and Antioxidant Activity of 1H-Benzimidazole-2-Yl Hydrazones
Authors: Argirova Maria , Guncheva Maya , Momekov Georgi , Cherneva Emiliya , Mihaylova Rositsa , Rangelov Miroslav , Todorova Nadezhda , Denev Petko , Anichina Kameliya , Mavrova Anelia , Yancheva Denitsa
Published: December 2022

1H-benzimidazol-2-yl hydrazones with varying hydroxy and methoxy phenyl moieties were designed. Their effect on tubulin polymerization was evaluated in vitro on porcine tubulin. The compounds elongated the nucleation phase and slowed down the tubulin polymerization comparably to nocodazole. The possible binding modes of the hydrazones with tubulin were explored by molecular docking at the colchicine binding site. The anticancer activity was evaluated against human malignant cell lines MCF-7 and AR-230, as well as against normal fibroblast cells 3T3 and CCL-1. The compounds demonstrated a marked antineoplastic activity in low micromolar concentrations in both screened in vitro tumor models. The most active were the trimethoxy substituted derivative 1i and the positional isomers 1j and 1k, containing hydroxy and methoxy substituents: they showed IC50 similar to the reference podophyllotoxin in both tumor cell lines, accompanied with high selectivity towards the malignantly transformed cells. The compounds exerted moderate to high ability to scavenge peroxyl radicals and certain derivatives—1l containing metha-hydroxy and para-methoxy group, and 1b-e with di/trihydroxy phenyl moiety, revealed HORAC values high or comparable to those of well-known phenolic antioxidants. Thus the 1H-benisimidazol-2-yl hydrazones with hydroxy/methoxy phenyl fragments were recognized as new agents exhibiting promising combined antioxidant and antineoplastic action.
Preparation of Salt-Induced Ultra-Stretchable Nanocellulose Composite Hydrogel for Self-Powered Sensors
Authors: Wang Xiaofa , Li Xincai , Wang Baobin , Chen Jiachuan , Zhang Lei , Zhang Kai , He Ming , Xue Yu , Yang Guihua
Published: December 2022

Hydrogels have attracted much attraction for promising flexible electronics due to the versatile tunability of the properties. However, there is still a big obstacle to balance between the multi-properties and performance of wearable electronics. Herein, we propose a salt-percolated nanocellulose composite hydrogel which was fabricated via radical polymerization with acrylic acid as polymer networks (NaCl-CNCs-PAA). CNCs were utilized as a reinforcing agent to enhance the mechanical properties of the hydrogel. Moreover, the abundant hydroxyl groups endow the hydrogel with noncovalent interactions, such as hydrogen bonding, and the robustness of the hydrogel was thus improved. NaCl incorporation induced the electrostatic interaction between CNCs and PAA polymer blocks, thus facilitating the improvement of the stretchability of the hydrogel. The as-obtained hydrogel exhibited excellent stretchability, ionic conductivity, mechanical robustness and anti-freezing properties, making it suitable for self-powered sensing applications. A single-mode triboelectric nanogenerator (C-TENG) was fabricated by utilizing the composite hydrogel as electrodes. This C-TENG could effectively convert biomechanical energy to electricity (89.2 V, 1.8 µA, 32.1 nC, and the max power density of 60.8 mW m−2 at 1.5 Hz.) Moreover, the composite hydrogel was applied for strain sensing to detect human motions. The nanocellulose composite hydrogel can achieve the application as a power supply in integrated sensing systems and as a strain sensor for human motion detection.
A Stretchable Expanded Polytetrafluorethylene-Silicone Elastomer Composite Electret for Wearable Sensor
Authors: Tan Jianbo , Chen Kaikai , Cheng Jinzhan , Song Zhaoqin , Zhang Jiahui , Zheng Shaodi , Xu Zisheng , E Shiju
Published: December 2022

Soaring developments in wearable electronics raise an urgent need for stretchable electrets. However, achieving soft electrets simultaneously possessing excellent stretchability, longevity, and high charge density is still challenging. Herein, a facile approach is proposed to prepare an all-polymer hybrid composite electret based on the coupling of elastomer and ePTFE membrane. The composite electrets are fabricated via a facile casting and thermal curing process. The obtained soft composite electrets exhibit constantly high surface potential (−0.38 kV) over a long time (30 days), large strain (450%), low hysteresis, and excellent durability (15,000 cycles). To demonstrate the applications, the stretchable electret is utilized to assemble a self-powered flexible sensor based on the electrostatic induction effect for the monitoring of human activities. Additionally, output signals in the pressure mode almost two orders of magnitude larger than those in the strain mode are observed and the sensing mechanism in each mode is investigated.
Foliar Fertilization by the Sol-Gel Particles Containing Cu and Zn
Authors: Borak Beata , Gediga Krzysztof , Piszcz Urszula , Sacała Elżbieta
Published: December 2022

Silica particles with the size of 150–200 nm containing Ca, P, Cu or Zn ions were synthesized with the sol-gel method and tested as a foliar fertilizer on three plant species: maize Zea mays, wheat Triticum sativum and rape Brassica napus L. var napus growing on two types of soils: neutral and acidic. The aqueous suspensions of the studied particles were sprayed on the chosen leaves and also on the whole tested plants. At a specific stage of plant development determined according to the BBCH (Biologische Bundesanstalt, Bundessortenamt und CHemische Industrie) scale, the leaves and the whole plants were harvested and dried, and the content of Cu and Zn was determined with the AAS (atomic absorption spectroscopy) method. The engineered particles were compared with a water solution of CuSO4 and ZnSO4 (0.1%) used as a conventional fertilizer. In many cases, the copper-containing particles improved the metal supply to plants more effectively than the CuSO4. The zinc-containing particles had less effect on both the growth of plants and the metal concentration in the plants. All the tested particles were not toxic to the examined plants, although some of them caused a slight reduction in plants growth.
Cardiometabolic Traits in Adult Twins: Heritability and BMI Impact with Age
Authors: Hong Xuanming , Wu Zhiyu , Cao Weihua , Lv Jun , Yu Canqing , Huang Tao , Sun Dianjianyi , Liao Chunxiao , Pang Yuanjie , Pang Zengchang , Cong Liming , Wang Hua , Wu Xianping , Liu Yu , Gao Wenjing , Li Liming
Published: December 2022

Background: The prevalence of obesity and cardiometabolic diseases continues to rise globally and obesity is a significant risk factor for cardiometabolic diseases. However, to our knowledge, evidence of the relative roles of genes and the environment underlying obesity and cardiometabolic disease traits and the correlations between them are still lacking, as is how they change with age. Method: Data were obtained from the Chinese National Twin Registry (CNTR). A total of 1421 twin pairs were included. Univariate structural equation models (SEMs) were performed to evaluate the heritability of BMI and cardiometabolic traits, which included blood hemoglobin A1c (HbA1c), fasting blood glucose (FBG), systolic blood pressure (SBP), diastolic blood pressure (DBP), total cholesterol (TC), triglycerides (TGs), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C). Bivariate SEMs were used to assess the genetic/environmental correlations between them. The study population was divided into three groups for analysis: ≤50, 51–60, and >60 years old to assess the changes in heritability and genetic/environmental correlations with ageing. Results: Univariate SEMs showed a high heritability of BMI (72%) and cardiometabolic traits, which ranged from 30% (HbA1c) to 69% (HDL-C). With age increasing, the heritability of all phenotypes has different degrees of declining trends. Among these, BMI, SBP, and DBP presented significant monotonous declining trends. The bivariate SEMs indicated that BMI correlated with all cardiometabolic traits. The genetic correlations were estimated to range from 0.14 (BMI and LDL-C) to 0.39 (BMI and DBP), while the environmental correlations ranged from 0.13 (BMI and TC/LDL-C) to 0.31 (BMI and TG). The genetic contributions underlying the correlations between BMI and SBP and DBP, TC, TG, and HDL-C showed a progressive decrease as age groups increased. In contrast, environmental correlations displayed a significant increasing trend for HbA1c, SBP, and DBP. Conclusions: The findings suggest that genetic and environmental factors have essential effects on BMI and all cardiometabolic traits. However, as age groups increased, genetic influences presented varying degrees of decrement for BMI and most cardiometabolic traits, suggesting the increasing importance of environments. Genetic factors played a consistently larger role than environmental factors in the phenotypic correlations between BMI and cardiometabolic traits. Nevertheless, the relative magnitudes of genetic and environmental factors may change over time.
Ameliorative Effects of Bifidobacterium animalis subsp. lactis J-12 on Hyperglycemia in Pregnancy and Pregnancy Outcomes in a High-Fat-Diet/Streptozotocin-Induced Rat Model
Authors: Yang Jianjun , Ma Yumeng , Li Tong , Pang Yuanxiang , Zhang Hongxing , Xie Yuanhong , Liu Hui , Sun Yanfang , Ren Jianhua , Jin Junhua
Published: December 2022

Bifidobacterium, a common probiotic, is widely used in the food industry. Hyperglycemia in pregnancy has become a common disease that impairs the health of the mother and can lead to adverse pregnancy outcomes, such as preeclampsia, macrosomia, fetal hyperinsulinemia, and perinatal death. Currently, Bifidobacterium has been shown to have the potential to mitigate glycolipid derangements. Therefore, the use of Bifidobacterium-based probiotics to interfere with hyperglycemia in pregnancy may be a promising therapeutic option. We aimed to determine the potential effects of Bifidobacterium animalis subsp. lactis J-12 (J-12) in high-fat diet (HFD)/streptozotocin (STZ)-induced rats with hyperglycemia in pregnancy (HIP) and respective fetuses. We observed that J-12 or insulin alone failed to significantly improve the fasting blood glucose (FBG) level and oral glucose tolerance; however, combining J-12 and insulin significantly reduced the FBG level during late pregnancy. Moreover, J-12 significantly decreased triglycerides and total cholesterol, relieved insulin and leptin resistance, activated adiponectin, and restored the morphology of the maternal pancreas and hepatic tissue of HIP-induced rats. Notably, J-12 ingestion ameliorated fetal physiological parameters and skeletal abnormalities. HIP-induced cardiac, renal, and hepatic damage in fetuses was significantly alleviated in the J-12-alone intake group, and it downregulated hippocampal mRNA expression of insulin receptor (InsR) and insulin-like growth factor-1 receptor (IGF-1R) and upregulated AKT mRNA on postnatal day 0, indicating that J-12 improved fetal neurological health. Furthermore, placental tissue damage in rats with HIP appeared to be in remission in the J-12 group. Upon exploring specific placental microbiota, we observed that J-12 affected the abundance of nine genera, positively correlating with FBG and leptin in rats and hippocampal mRNA levels of InsR and IGF-1R mRNA in the fetus, while negatively correlating with adiponectin in rats and hippocampal levels of AKT in the fetus. These results suggest that J-12 may affect the development of the fetal central nervous system by mediating placental microbiota via the regulation of maternal-related indicators. J-12 is a promising strategy for improving HIP and pregnancy outcomes.