bluuebunny/crossref_metadata_2025 huggingface dataset:
Preparation and Preliminary Application of Epitope Peptide-Based Antibody against Toxoplasma gondii GRA3
Authors: Wang Ru , Wu Minmin , Cai Haijian , An Ran , Chen Ying , Wang Jie , Zhou Nan , Du Jian
Published: February 2023

Toxoplasma gondii dense granule protein GRA3 has been shown to promote Toxoplasma gondii transmission and proliferation by interacting with the host cell endoplasmic reticulum (ER) through calcium-regulated cyclophilin ligands (CAMLG). Although many studies have focused on the interaction between the host cell endoplasmic reticulum and GRA3, no polyclonal antibodies (PcAbs) against GRA3 have been reported to date. According to the antigenicity prediction and exposure site analysis, three antigen peptide sequences were selected to prepare polyclonal antibodies targeting GRA3. Peptide scans revealed that the major antigenic epitope sequences were 125ELYDRTDRPGLK136, 202FFRRRPKDGGAG213, and 68NEAGESYSSATSG80, respectively. The GRA3 PcAb specifically recognized the GRA3 of T. gondii type Ⅱ ME49. The development of PcAbs against GRA3 is expected to elucidate the molecular mechanisms by which GRA3 regulates host cell function and contribute to the development of diagnostic and therapeutic strategies for toxoplasmosis.
Ecomindsponge: A Novel Perspective on Human Psychology and Behavior in the Ecosystem
Authors: Nguyen Minh-Hoang , Le Tam-Tri , Vuong Quan-Hoang
Published: February 2023

Modern society faces major environmental problems, but there are many difficulties in studying the nature–human relationship from an integral psychosocial perspective. We propose the ecomind sponge conceptual framework, based on the mindsponge theory of information processing. We present a systematic method to examine the nature–human relationship with conceptual frameworks of system boundaries, selective exchange, and adaptive optimization. The theoretical mechanisms were constructed based on principles and new evidence in natural sciences. The core mechanism of ecomindsponge is the subjective sphere of influence, which is the limited mental representation of information received from and processed based on the objective sphere of influence–actual interactions in reality. The subjective sphere is the sum of two sub-spheres: influencing (proactive) and being influenced (reactive). Maladaptation in thinking and behavior of the mind as an information collection-cum-processor results from the deviation of the subjective sphere from reality, which includes two main types: “stupidity” and “delusion”. Using Bayesian Mindsponge Framework (BMF) analytics on a dataset of 535 urban residents, we provide consistent statistical evidence on the proposed properties of subjective spheres. The dynamic framework of ecomindsponge can be used flexibly and practically for environmental research as well as other psychosocial fields.
LINE1-Mediated Reverse Transcription and Genomic Integration of SARS-CoV-2 mRNA Detected in Virus-Infected but Not in Viral mRNA-Transfected Cells
Authors: Zhang Liguo , Bisht Punam , Flamier Anthony , Barrasa M. Inmaculada , Friesen Max , Richards Alexsia , Hughes Stephen H. , Jaenisch Rudolf
Published: February 2023

SARS-CoV-2 sequences can be reverse-transcribed and integrated into the genomes of virus-infected cells by a LINE1-mediated retrotransposition mechanism. Whole-genome sequencing (WGS) methods detected retrotransposed SARS-CoV-2 subgenomic sequences in virus-infected cells overexpressing LINE1, while an enrichment method (TagMap) identified retrotranspositions in cells that did not overexpress LINE1. LINE1 overexpression increased retrotranspositions about 1000-fold as compared to non-overexpressing cells. Nanopore WGS can directly recover retrotransposed viral and flanking host sequences, but its sensitivity depends on the depth of sequencing (a typical 20-fold sequencing depth would only examine 10 diploid cell equivalents). In contrast, TagMap enriches the host–virus junctions and can interrogate up to 20,000 cells and is able to detect rare viral retrotranspositions in LINE1 non-overexpressing cells. Although Nanopore WGS is 10–20-fold more sensitive per tested cell, TagMap can interrogate 1000–2000-fold more cells and, therefore, can identify infrequent retrotranspositions. When comparing SARS-CoV-2 infection and viral nucleocapsid mRNA transfection by TagMap, retrotransposed SARS-CoV-2 sequences were only detected in infected but not in transfected cells. Retrotransposition in virus-infected cells, in contrast to transfected cells, may be facilitated because virus infection, in contrast to viral RNA transfection, results in significantly higher viral RNA levels and stimulates LINE1 expression by causing cellular stress.
Assessment of the Biological Impact of SARS-CoV-2 Genetic Variation Using an Authentic Virus Neutralisation Assay with Convalescent Plasma, Vaccinee Sera, and Standard Reagents
Authors: Coombes Naomi S. , Bewley Kevin R. , Le Duff Yann , Hurley Matthew , Smith Lauren J. , Weldon Thomas M. , Osman Karen , Pullan Steven , Berry Neil , Hallis Bassam , Charlton Sue , Hall Yper , Funnell Simon G. P.
Published: February 2023

In the summer of 2020, it became clear that the genetic composition of SARS-CoV-2 was changing rapidly. This was highlighted by the rapid emergence of the D614G mutation at that time. In the autumn of 2020, the project entitled “Agility” was initiated with funding from the Coalition for Epidemic Preparedness Innovations (CEPI) to assess new variants of SARS-CoV-2. The project was designed to reach out and intercept swabs containing live variant viruses in order to generate highly characterised master and working stocks, and to assess the biological consequences of the rapid genetic changes using both in vitro and in vivo approaches. Since November 2020, a total of 21 variants have been acquired and tested against either a panel of convalescent sera from early in the pandemic, and/or a panel of plasma from triple-vaccinated participants. A pattern of continuous evolution of SARS-CoV-2 has been revealed. Sequential characterisation of the most globally significant variants available to us, generated in real-time, indicated that the most recent Omicron variants appear to have evolved in a manner that avoids immunological recognition by convalescent plasma from the era of the ancestral virus when analysed in an authentic virus neutralisation assay.
High-Resolution Genomic Profiling of a Genotype 3b Hepatitis C Virus from a Flare of an Occult Hepatitis Patient with Acute-on-Chronic Liver Failure
Authors: Mei Xue , Zou Jingyi , Shi Bisheng , Qian Zhiping , Yi Zhigang
Published: February 2023

Acute-on-chronic liver failure (ACLF) is defined as a syndrome of acutely decompensated cirrhosis in patients with chronic liver disease (CLD). Here we report an ACLF case caused by a flare of occult hepatitis C infection. This patient was infected with hepatitis C virus (HCV) more than a decade ago and hospitalized due to alcohol-associated CLD. Upon admission, the HCV RNA in the serum was negative and the anti-HCV antibody was positive, whereas the viral RNA in the plasma dramatically increased during hospitalization, which suggests an occult hepatitis C infection. Overlapped fragments encompassing the nearly whole HCV viral genome were amplified, cloned, and sequenced. Phylogenetic analysis indicated an HCV genotype 3b strain. Sanger sequencing to 10-fold coverage of the 9.4-kb nearly whole genome reveals high diversity of viral quasispecies, an indicator of chronic infection. Inherent resistance-associated substitutions (RASs) in the NS3 and NS5A but not in the NS5B regions were identified. The patient developed liver failure and accepted liver transplantation, followed by direct-acting antiviral (DAA) treatment. The hepatitis C was cured by the DAA treatment despite the existence of RASs. Thus, care should be taken for occult hepatitis C in patients with alcoholic cirrhosis. The analysis of viral genetic diversity may help to identify an occult hepatitis C virus infection and predict the efficacy of anti-viral treatment.
Atmospheric Exchange of Carbon Dioxide and Water Vapor above a Tropical Sandy Coastal Plain
Authors: Jia Jun-Ting , Xue Yang , Zhao Jun-Fu , Yang Zhong-Yang , Su Shao-Feng , Wang Xiao-Yan , Lin Zhi-Pan , Wang Guan-Ze , Yang Lian-Yan , Zhang Xiang
Published: February 2023

As a unique type of ecosystem, tropical coastal sandy vegetation lies in the transition zone extending from coastal beaches to further inland and provides important ecosystem services such as windproofing, tourism, and agriculture. However, the energy and matter fluxes of these tropical coastal ecosystems have been rarely studied. We reported one-year eddy flux observations in a tropical sandy coastal ecosystem and specifically focused on the carbon and water exchanges between the atmosphere and the ecosystem. The studied ecosystem was a carbon sink (approximately –560 gC m−2 yr−1) and approximately 1000 mm of water evaporated from the ecosystem into the atmosphere during the study year. The highest levels of vegetation photosynthesis occurred in April, shortly before the wet season. This can be attributed to an endogenous self-adjustment of the ecosystem to improve the water- and carbon-use efficiency during the wet season. This study is expected to not only fill the data gap with respect to the gas exchange between tropical sandy coastal plains and the atmosphere but also provide knowledge about the function and ecological service of these specific ecosystems.
The Interaction of Aeolian Sand and Slope on Runoff and Soil Loss on a Loess Slope via Simulated Rainfall under Laboratory Conditions
Authors: Ren Zongping , Pan Jinjin , Li Zhanbin , Xiao Peiqing , Shen Zhenzhou , Jia Lu , Li Xiaozheng
Published: February 2023

The wind–water erosion crisscross region, where the topography is complicated, is the most severe area of soil erosion on the Loess Plateau. The wind and terrain both have an impact on the soil water erosion process. In order to evaluate the effects of sand cover on runoff and soil loss characteristics, a series of experiments was conducted in two contrasting treatments. One treatment was a bare loess soil slope serving as the control, and the others were sand-covered loess slopes with five different slopes. The results showed that the runoff time, total runoff yield, and total soil loss were different between the sand-covered slope and the loess slope on the slope of 15°. The sediment concentration of the sand-covered slope was significantly higher than that of the loess slope during the entire rainfall process (p < 0.05). The increase in the slope gradient shortened the surface runoff initiation times and enhanced the total runoff volume and soil loss. The total runoff volume and the total soil loss were 39.7 L and 44.3 kg, respectively, on the sand-covered slope of 10°. When the slope gradient increased from 10° to 30°, the total runoff volume and the total soil loss increased by 22.8 L and 42.8 kg, respectively, while the surface runoff initiation times shortened by 300 s. For the sand-covered slopes, the erosion processes appeared to be mainly dominated by sediment transport. The correlation between soil loss rates and slope gradients demonstrated the secondary polynomial function. In addition, the critical slope of sand-covered slopes was from approximately 23° to 28°. The proportion of sand cover and slope responsible for soil erosion was 3:1, which means the wind effect was more important than the terrace factor in terms of soil water erosion in the wind–water erosion crisscross region. The results provide a theoretical basis for soil erosion control in this area.
Determination of Exploitable Coefficient of Coral Island Freshwater Lens Considering the Integrated Effects of Lens Growth and Contraction
Authors: Wang Ran , Shu Longcang , Zhang Rongrong , Ling Zihan
Published: February 2023

Groundwater on small coral islands (so-called freshwater lens) is an important water resource for residents and local ecosystems. However, an overexploitation of it may induce a contamination by saltwater. In this paper, we strive to determine the exploitable coefficient of the freshwater lens considering the integrated effects of lens growth and contraction and examine the impacts of well layout schemes on the evolution of the freshwater lens. For this purpose, a numerical model is setup to simulate the saltwater upconing and recovery process under pumping conditions during different evolution stages. Our results show that long-term and higher intensity pumping activities are suggested to be conducted at the latter stage of the lens evolution. Meanwhile, the seasonal contraction of the freshwater lens caused by the seasonal variation in rainfall is characterized by a quicker response of center thickness than maximum thickness of the lens, which further impacts the pumping intensity. The results also indicate that the exploitable coefficient (ρ) of the freshwater lens in small coral island is generally smaller than that in inland areas, ranging from 0.09 to 0.37 under different well layout schemes. Additionally, it is also affected by the uncertainty of hydrogeological parameters. Finally, a safe exploitable coefficient is proposed under the most unfavorable parameter combination for the studied island. The study has important implications for the protection and sustainable exploitation of subsurface freshwater resources on island.
Sea, River, Lake Ice Properties and Their Applications in Practices
Authors: Li Zhijun , Kolerski Tomasz , Zhou Li , Shi Xiaohong , Zhang Zhengyong , Li Fang
Published: February 2023

This Special Issue aims to highlight research articles focusing on the geographical scale of glacier and lake ice variations, as well as the engineering scale of ice properties and their practical applications via laboratory tests and numerical modeling. Additionally, it includes research on ecosystems under lake ice. The main goal is successfully achieved through the joint efforts of authors, anonymous reviewers, and editorial managers. In total, 1 review article and 15 research articles are included in this Special Issue. These articles cover a wide range of topics, including water resources from Chinese mountain glacier variation; lake ice phenology at different latitudes and altitudes around the world; ice properties from laboratory experiments and numerical modeling; ice engineering with different purposes in China and the Arctic; and ecosystem under lake ice at different temporal and spatial scales. This Special Issue received contributions from researchers from different parts of China and from Chinese international cooperation partners because of its focus on “higher temperature ice” under global warming. All papers presented are innovative and of high quality. This Special Issue can promote research on ice properties and their applications in practices ranging from mountains to sea, especially in popular water ecosystem environments under ice during seasonal ice period.
Unravelling Nutrients and Carbon Interactions in an Urban Coastal Water during Algal Bloom Period in Zhanjiang Bay, China
Authors: Zhang Jibiao , Fu Miaojian , Zhang Peng , Sun Dong , Peng Demeng
Published: February 2023

Nutrients and carbon play important roles in algal bloom and development. However, nutrients and carbon interactions in the period of the spring algal bloom are not well understood. The aim of this study is to explore the nutrients and carbon interactions in the period of the spring algal bloom covering an urban Jinsha Bay (JSB) coastal water in Zhanjiang Bay (South China Sea) using in situ multidiscipline observation. The results showed that the average concentration of total nitrogen (TN), total phosphorus (TP), and dissolved silicon (DSi) was 97.79 ± 26.31 μmol/L, 12.84 ± 4.48 μmol/L, and 16.29 ± 4.00 μmol/L in coastal water, respectively. Moreover, the average concentration of total dissolved carbon (TDC), dissolved inorganic carbon (DIC) and organic carbon (DOC) in JSB was 2187.43 ± 195.92 μmol/L, 1516.25 ± 133.24 μmol/L, and 671.13 ± 150.81 μmol/L, respectively. Furthermore, the main dominant species were Phaeocystis globosa and Nitzschia closterium during the spring algal bloom. Additionally, the correlation analysis showed salinity (S) was significantly negatively correlated with nutrients, indicating that nutrients derived from land-based sources sustained spring algal bloom development. However, as the major fraction of TDC, DIC was significantly positively correlated with S, which was mainly derived from marine sources. Besides, the algal density showed a significant positive correlation with temperature (T) (p < 0.001) and dissolved oxygen (DO) (p < 0.001), but a significant negative correlation with DIC (p < 0.05), suggesting that spring algal blooms may be simulated by water T increase, and then large amounts of DIC and nutrients were adsorbed, accompanying DO release through photosynthesis in coastal water. This study revealed nutrients and carbon interactions in the spring algal bloom of urban eutrophic coastal water, which has implications for understanding the nutrients and carbon biogeochemical cycle and algal bloom mitigation under climate change and anthropogenic pressures in the future.
Comparison Study on Climate Changes between the Guangdong–Hong Kong–Macao Greater Bay Area and Areas around the Baltic Sea
Authors: Wang Bing , Zhang Jinpeng , Yang Jie , Zheng Jing , Xu Yanhong , Chai Wenguang
Published: February 2023

With global warming, coastal areas are exposed to multiple climate-related hazards. Understanding the facts and attribution of regional climate change in coastal communities is a frontier science challenge. In this study, we focus on fact analysis of multi-scale climate changes in the Guangdong–Hong Kong–Macao Greater Bay area (GBA) and around the Baltic Sea area (BSA). We selected three Asian stations from the GBA in South China (Guangzhou, Hong Kong, and Macao) and five European stations around the Baltic Sea (Stockholm, Haparanda A, Vestervig, Poznan, and Frankfurt) from four countries in the BSA as representative stations, which have more than 100- or 150-year datasets. Based on the ensemble empirical mode decomposition (EEMD) and Mann–Kendall methods, this study focuses on the multi-scale temperature and precipitation fluctuation and mutation analysis in the past. The multi-scale analyses show that there are four time-scale changes in both areas. They are the inter-annual scale, inter-decadal scale, centennial scale, and trend, but the lengths of different timescales vary in both regions, especially the inter-decadal scale and centennial scale. For temperature, the inter-annual scales show the same results, with 2–4 and 7–9 a in both the GBA and BSA. In the GBA, the inter-decadal scales are 10–14, 30–50, and 55–99 a, while in the BSA, they are 13–20, 26–50, and 66–99 a. For centennial scales, there are 143–185 and 200–264 a in the BSA and about 100–135 a in the GBA. Temperature trends in the GBA reveal that the coastal area has experienced an upward trend (Hong Kong and Macao), but in the inland area (Guangzhou), the trend fluctuated. Temperature trends in the BSA have risen since 1756. For precipitation, the inter-annual scales are 2–4 and 6–9 a in both the GBA and BSA. The inter-decadal scales are 11–29 and 50–70 a in the GBA and 11–20, 33–50, and 67–86 a in the BSA. For centennial scales, there are about 100 a in the GBA and 100–136 a in the BSA. In the GBA, the precipitation trends show stronger local characteristics, with three different fluctuation types. In the BSA, most stations had a fluctuating trend except Haparanda A and Vestervig station, which experienced an upward trend throughout the whole time range. Overall, there are no unified trends for precipitation in both areas. Temperature mutation tests show that only Vestervig in the BSA changed abruptly in 1987, while the mutation point of Macao in the GBA was 1991. Precipitation mutation points of Stockholm and Vestervig were 1878 and 1918 in the BSA, while only Macao in the GBA changed abruptly in 1917. The results reveal that the regional climate mutation of both areas is not obvious, but the temperature changes with an upward trend as a whole.
Análise da frequência fundamental vocal de pacientes com acromegalia e correlação com a anatomia laríngea
Authors: Da Silva Jéssyca Rodrigues , Carvalho João Victor Sousa , Da Silva Joissilane Rodrigues , Lima Rayza Gregório , Lacerda Talita Oliveira do Vale , Ribeiro Natasha Cortez Silva , Said Mira Nabil , Júnior Roberto Farias Lopes , Da Costa Ediane Cristina Carrera Eleres , Caldato Milena Coelho Fernandes , Nunes Leonardo Mendes Acatauassú
Published: February 2023

A acromegalia ocorre com a mesma frequência em homens e mulheres podendo ser diagnosticada em qualquer faixa etária, porém o diagnóstico é mais comum entre os 30 e 50 anos de idade. A alteração nos parâmetros de qualidade vocal, frequência, intensidade ou esforço vocal pode resultar em rouquidão, uma das repercussões da acromegalia, condição que limita a comunicação ou causa impacto negativo na qualidade de vida relacionada à voz. Estudo observacional do tipo transversal comparativo, com abordagem quantitativa descritiva e analítica, onde a frequência fundamental vocal de 17 pacientes com acromegalia e 34 controles foram analisadas por software de voz e realizada videolaringoscopia em 17 pacientes com diagnóstico de acromegalia. Foram incluídos no presente estudo 17 (33.3%) pacientes com acromegalia (GA) e 34 (66.7%) sem a doença (GC), com idade e proporção de homens e mulheres semelhantes entre os grupos.  A frequência fundamental vocal (Fo) mínima, máxima e média apresentou-se distribuída homogeneamente entre os pacientes com acromegalia, porém entre os pacientes do grupo controle foram observadas variações significativas nessas distribuições. A comparação dos parâmetros vocais entre os grupos GA e GC revelou, na média, Fo máxima superior nos pacientes GC, enquanto a comparação segundo o gênero, foi observada Fo mínima com mediana superior entre os homens do grupo GA e valor mediano de Fo máxima superior entre as mulheres do grupo GC. Observou-se associação entre maiores valores medianos da Fo mínima nos pacientes com redução do diâmetro anteroposterior do arcabouço laríngeo e entre os que possuíam sinais de refluxo. A Fo de pacientes com acromegalia é menor do que do grupo controle. A laringe dos pacientes com diagnóstico da doença apresenta diminuição
Análise eletromiográfica e estabilométrica em crianças com paralisia cerebral após um protocolo intensivo de exercícios terapêuticos
Authors: Boligon Gabrieli Vieira , Perissé Beatriz França Naves , Costa Alicya Victória González , Machado Felipe Alves , Oliveira Ana Letícia de Souza
Published: February 2023

Introdução: A Paralisia Cerebral (PC) é uma condição de saúde comum da infância, em que trata-se de lesões neurológicas que acarretam atrasos no desenvolvimento neurosensoriomotor, cognitivo e, muitas vezes, provoca déficits no desempenho funcional e equilíbrio postural. Um novo recurso de tratamento para essas crianças são os protocolos intensivos com o uso ou não de vestimentas terapêuticas. Nestes protocolos, as vestimentas funcionam como uma órtese corporal que permite a execução de movimentos e exercícios para os mais diversos objetivos, em conjunto com a aplicação de treinos intensos e específicos. Objetivo: O objetivo do presente estudo foi analisar a diferença de ativação muscular, função motora grossa e oscilação do centro de gravidade de crianças com PC após um protocolo intensivo de exercícios terapêuticos. Tratou-se de um estudo de 10 casos, sendo que os critérios de inclusão foram ter idade entre 2 e 13 anos, diagnóstico de PC e classificação Gross Motor Function Classification System entre os níveis I e IV. Métodos: A Gross Motor Function Measure foi realizada por um profissional capacitado antes do primeiro e após o último protocolo. A Eletromiografia de Superfície foi utilizada para analisar a ativação muscular dos músculos Reto Abdominal, Oblíquos e Multífidos de ambos hemicorpos enquanto a criança permanecia sentada mantendo a postura durante 30 segundos pré e pós protocolo. Além disso, a Plataforma Estabilométrica foi utilizada para avaliar a oscilação postural com a criança sentada mantendo a posição por 30 segundos com os olhos abertos e 30 segundos com os olhos fechados, também antes do início do intensivo e após o último. Resultados: No geral, as crianças apresentaram aumento da média na função motora grossa em 4,68% entre antes e após o treinamento; 30% dos pacientes apresentaram aumento da ativação muscular de todas ou da maioria das musculaturas; 40% obtiverem uma maior aproximação das médias de Root Mean Square associados a uma redução de ativação em alguns dos 6 músculos avaliados. Em relação ao centro de gravidade, 30% dos pacientes reduziram a distância de oscilação com olhos abertos e 70% reduziram com os olhos fechados. Conclusão: Conclui-se com o presente estudo que a realização de um protocolo intensivo de exercícios terapêuticos associados ou não ao uso de vestimentas terapêuticas favorece a melhora da função motora grossa, parece ser promissor na ativação mais equilibrada entre as musculaturas do core avaliadas e na diminuição da oscilação do tronco quando de olhos fechados.
Contribuições do estágio obrigatório em disfunções osteomioarticulares na formação acadêmica de graduandos de fisioterapia do 5º ano: relato de experiência
Authors: Torres Luciano Gil Saldanha , Da Silva Michelly Beatriz Fernandes , Sassim Paulo Vitor de Souza , Guimarães Andréa de Cassia Lima
Published: February 2023

Objetivo: descrever as contribuições do estágio nas disfunções osteomioarticulares na formação acadêmica de graduandos do curso de fisioterapia do 5º ano da Universidade do Estado do Pará. Metodologia: Trata-se de um estudo do tipo relato de experiência de caráter descritivo com abordagem qualitativa, realizado por acadêmicos do 5° ano de curso de Fisioterapia da Universidade do Estado do Pará, na Unidade de Ensino e Assistência em Fisioterapia e Terapia Ocupacional, localizada no bairro do Marco em Belém-Pará, desenvolvido a partir da vivência do estágio obrigatório no eixo “Fisioterapia Ambulatorial nas Disfunções Osteomioaticulares. Resultados e discussão: No início das atividades de estágio os discentes efetuaram as avaliações dos pacientes que seriam atendidos por meio de uma ficha própria utilizada no estágio. As avaliações ocorreram sob a supervisão da docente que intervia quando os alunos apresentavam dificuldades na avaliação, como perguntas ao paciente e posturas do exame físico. Desse modo, foi evidente durante o estágio a percepção dos acadêmicos sobre o ganho de independência em relação a comunicação com os pacientes, avaliação e evolução deles dentro do plano de tratamento traçado, uma vez que nos estágios anteriores, durante o 4º ano, havia a intervenção direta do professor durante os atendimentos, além da presença do monitor que complementava as lacunas que ainda não tinham sido aprimoradas pelos acadêmicos. Considerações finais: Os acadêmicos tiveram a chance de obter maior experiência e independência para a futura prática profissional, além do aprimoramento do raciocínio clínico para condução do tratamento para a melhora dos pacientes sendo notório a segurança de atuar de forma mais independente ao final do estágio.
МУЗИЧНО-ПЕДАГОГІЧНА ОСВІТА В ПРОВІНЦІЇ ЧЖЕЦЗЯН
Authors: Іцзян Го
Published: February 2023

Розкрито досвід організації музично-педагогічної освіти трьох найбільш потужних закладів вищої освіти провінції Чжецзян у Китайській Народній Республіці: Чжецзянскої музичної консерваторії, Чзецзянського нормального (педагогічного) університету та Ханчжоуського нормального (педагогічного) університету. З’ясовано, що всі три виші мають у своїй структурі окремі підрозділи, чия діяльність сконцентрована на реалізації мистецької та мистецько-педагогічної освіти: коледжі та школи мистецтв, зокрема, школи музики. Виявлено, що всі перелічені університети та мистецькі школи у своїй структурі мають найновіше матеріально-технічне забезпечення, достатню кількість приміщень для мистецько-педагогічного процесу, творчості та презентації її результатів. Аналіз освітньої програми за спеціальністю Музикознавство (викладання) бакалаврського рівня вищої освіти Школи музики Ханчжоуського педагогічного університету дав підстави для висновку про увагу її розробників до деталізації цілей і результатів навчання за освітніми програмами музично-педагогічного спрямування. Конкретні вимоги до випускника (результати навчання в українській педагогічній термінології) об’єднані в 4 групи: 1) дієва мораль вчителя; 2) вміння навчати; 3) вміння виховувати; 4) розвиток та інновації. Програма наводить до певної міри розгорнуту характеристику кожної групи і конкретизує її. Привертає увагу урізноманітнення переліку навчальних дисциплін, особливо дисциплін вільного вибору здобувачів освіти, не тільки активне застосування сучасної цифрової техніки в освітньому процесі, але й навчання майбутніх музикантів-педагогів впевнено користуватися такими технічними можливостями у своїй творчій і педагогічній діяльності. Для китайських педагогів має стати корисним досвід українських колег з організації різноманітних видів практик у процесі реалізації освітніх програм музичнопедагогічного спрямування, розробки освітніх програм та їх науково-методичного супроводу.
Nuevo registro de Haemagogus (Haemagogus) equinus Theobald, 1903 y otros mosquitos (Diptera: Culicidae) recolectados con ovitrampas en Jalpan de Serra, Querétaro, México
Authors: Quezada-Yaguachi Walter Eduardo , Alquisira-Domínguez Miriam , VázquezAnzúres Miriam J. , Rebollo-Salinas Dania , Rescalvo-Luna L. Dalila , Medina-Castañeda Armando , González-Acosta Cassandra , Correa-Morales Fabián , Viveros-Santos Vicente , Moreno-García Miguel
Published: February 2023

El cambio en la estructura de los paisajes por la actividad humana ha aumentado la probabilidad de contacto con mosquitos silvestres capaces de adaptarse a nuevos hábitats. El monitoreo de sus poblaciones en zonas urbanas mediante ovitrampas ha permitido actualizar la distribución y el conocimiento de la riqueza de especies. Este estudio reporta las especies recolectadas con ovitrampas en Jalpan de Serra, Querétaro, México, localidad adyacente a la Reserva “Sierra Gorda”. Se colectaron 5.580 papeletas de oviposición colocadas aleatoriamente en aéreas peridomiciliares. En laboratorio los huevos eclosionaron y las larvas fueron criadas hasta estado adulto. Un total de 5.956 adultos de cinco géneros fueron determinados taxonómicamente. De manera oficial se reporta la presencia de Haemagogus equinus en el estado de Querétaro. Esta especie es reconocida como vector de fiebre amarilla, con importancia médica y veterinaria debido a ciclos urbanos y selváticos. Adicionalmente, se reporta a Aedes aegypti, Ae. albopictus, Ae. epactas y Ae. podographicus, las dos primeras con relevancia en salud pública. El monitoreo, vigilancia y reporte de mosquitos con ovitrampas en zonas perturbadas son de suma importancia para generar propuestas de investigación que eviten la emergencia de enfermedades transmitidas por vectores.
A INFLUÊNCIA DAS RELAÇÕES INTERPESSOAIS PARA A AQUISIÇÃO DO ESTRESSE INFANTIL
Authors: Silva de Macedo Clayto
Published: February 2023

This is a bibliographic review about the incidence of stress on the child population. A bibliographic survey was carried out in the database of referenced sites for academic research, mainly with the keywords: stress, child and school. Observing the period of publications between 1990 and 2014, a total of 12 articles . Literature points out to the importance of the knowledge of childhood stress by the family, teachers and all school staff so that they may be able to act correctly with the child and thus provide a healthy individual development that will affect society as a whole.
Implikasi Perkembangan Kognitif dalam Pembelajaran di Sekolah Dasar
Authors: Yunaini Norma , Yuyun Winingsih Devi
Published: February 2023

Tujuan penelitian ini adalah untuk mengkaji lebih jauh tentang teori perkembangan kognitif oleh Jean Piaget terhadap pembelajaran di sekolah dasar sesuai tingkat berfikir anak. Sumber data yang digunakan dalam penelitian ini yaitu buku, jurnal, artikel, dan karya ilmiah lainnya. Teknik pengumpulan datanya adalah Studi Pustaka. Analisis data menggunakan content analysis. Hasil penelitian ini menunjukkan bahwa perkembangan kognitif anak pada tahap usia operasional konkret (7-12 tahun) dalam pembelajaran ini berbeda-beda hampir pada setiap fase usianya. Pembelajaran di Sekolah Dasar disesuaikan dengan tahapan usia. Hal ini sesuai dengan implementasi teori perkembangan Jean Piaget. Merujuk pada bagaimana orang tumbuh, menyesuaikan diri, dan berubah sepanjang perjalanan hidupnya melalui perkembangan fisik, perkembangan kepribadian, perkembangan sosioemosi, perkembangan kognisi (pemikiran), dan perkembangan bahasa. Selain tingkat pemahaman model dan metode serta penanganan yang digunakan juga bervariatif. Hasil penelitian dapat menjadi landasan guru mengajar sehingga pembelajaran menjadi lebih efektif, efisien, dan tepat sasaran. Terlebih untuk mewujudkan tercapainya tujuan pendidikan nasional
Pengembangan LKPD Berbasis Problem Based Learning untuk Meningkatkan Kemampuan Berpikir Kritis Siswa SD Kelas VI
Authors: Pitriyana Sisi , Karnita Arafatun Sasih
Published: February 2023

Latar belakang masalah penelitian ini adalah kemampuan berpikir kritis matematis peserta didik masih tergolong rendah. Hasil Pembelajaran matematika dikelas masih berpusat pada guru, peserta didik belajar hanya berpatokan pada bahan ajar yang digunakan dalam kegiatan pembelajaran di kelas, yaitu buku paket dari kemendikbud dan contoh yang diberikan. Ini menunjukkan di sekolah tersebut belum tersedia bahan ajar berupa Lembar Kerja Peserta Didik (LKPD). Selain itu, guru belum mengembangkan sendiri bahan ajar yang digunakan untuk menunjang kemampuan berpikir kritis Matematika Penelitian ini bertujuan untuk mengembangkan Lembar Kerja Peserta Didik (LKPD) Berbasis Problem Based Learning untuk meningkatkan kemampuan berpikir kritis peseta didik yang valid, praktis dan efektif. Metode penelitian ini adalah penelitian pengembangan (Research and Development). Langkah-langkah dalam membuat penelitian ini dilakukan dengan model pengembangan Addie (Analisys, Design, Development, Implementation, Evaluation). Subjek penelitian ini adalah siswa kelas VI SD Negeri I Pangkalpinang. Teknik Pengumpulan data yang digunakan yaitu angket, wawancara, Tes, dan Observasi. Penelitian ini menghasilkan Lembar Kerja Peserta Didik (LKPD) berbasis Problem Based Learning pada materi lingkaran siswa SD kelas VI. Hasil penelitian ini adalah Berdasarkan penilaian kevalidan dari ahli materi dan ahli media, LKPD yang dikembangkan telah memenuhi kriteria valid dengan kategori sangat baik. Berdasarkan dari respon peserta didik telah memenuhi kriteria praktis. Berdasarkan analisis jawaban siswa pada saat posttest maka LKPD matematika berbasis PBL efektif meningkatkan berpikir kritis matematika siswa kelas VI SD Negeri I Pangkalpinang.
Discursos sobre seguridad del gobierno del Frente Amplio
Authors:   , Pasturino Gabriela
Published: February 2023

Esta tesis estudia cómo se posicionó discursivamente el segundo gobierno del Frente Amplio (2010-2015) en Uruguay en relación a la seguridad, en momentos en que los discursos conservadores sobre el tema ganaron terreno en la región y en el país. Para abordar el corpus se elaboraron mapas taxonómicos, una herramienta del Análisis Crítico del Discurso (ACD) que permite organizar, jerarquizar y buscar relaciones entre ítem léxicos. Esta aproximación permitió analizar discursos oficiales producidos por distintos actores con responsabilidades políticas durante el año 2012. En particular, se buscó en el corpus la presencia de huellas discursivas de perspectivas conservadoras, la aparición de otras visiones sobre el tema, así como los acuerdos y contradicciones entre los textos analizados. Del análisis se desprende que conviven en los discursos oficiales del Frente Amplio huellas conservadoras, con otras perspectivas vinculadas a los derechos humanos, así como argumentos de tipo subculturales, anómicos, críticos, entre otros. La presencia de las visiones conservadoras del gobierno es más frecuente en los pasajes relacionados a propuestas para mejorar la seguridad y a los actores asociados al problema.
Marcadores de función vascular en pacientes con glucemia alterada en ayunas y con intolerancia a la glucosa
Authors:   , Martínez Camilo
Published: February 2023

Objetivos: Evaluar indicadores de rigidez arterial en personas con prediabetes (PreD) y su posible patogenia. Materiales y métodos: la velocidad de la onda del pulso (PWV) se midió en 208 personas con FINDRISC ≥ 13 (57 ± 8 años, 68,7% mujeres) y luego se dividieron en aquellas con tolerancia normal a la glucosa (NGT) o PreD. En cada subgrupo identificamos también a los que presentaban/no resistencia a la insulina (RI) medida por el cociente triglicéridos/HDL-c (valores de corte de normalidad previamente establecidos en nuestra población). Se recogieron datos clínicos y metabólicos de todos los participantes. PWV se comparó entre los subgrupos mediante la prueba t independiente. Resultados: Las mujeres y los hombres tenían características clínicas y metabólicas comparables con la obesidad (IMC ≥ 30) y el tratamiento con estatinas antihipertensivas, casi la mitad con NGT o PreD. Mientras que el 48 % de las personas con NGT presentaban IR (proporción TG/HDL-c anormalmente alta), el 52 % tenía PreD. PWV fue significativamente mayor solo en aquellos con un cuadro completo de síndrome metabólico (MS). Conclusiones: dado que la PWV se vio significativamente afectada en personas con un cuadro completo de EM, los médicos deben buscar cuidadosamente el diagnóstico temprano de esta afección y prescribir un estilo de vida saludable para prevenir el desarrollo/progresión de la ECV. Esta actitud proactiva proporcionaría una estrategia preventiva rentable para evitar el impacto negativo de las ECV en la calidad de vida de los pacientes y en los sistemas de salud debido a sus mayores costos de atención.
Uso de rubricas para análise do engajamento discente
Authors: Irala Valesca Brasil , Ribeiro Everton Silveira
Published: February 2023

Este é um relato de experiência com foco na metodologia problem-based learning (PBL) no formato post-holing. Foi executada durante o período de pandemia da covid-19, o que resultou na sua aplicação no modelo remoto. O principal objetivo desta pesquisa foi empreender uma análise qualitativa e descritiva, por meio de rubricas avaliativas, a respeito do engajamento discente em relação à aplicação do PBL. Para tal, foram desenhadas duas rubricas, as quais foram aplicadas ao final da intervenção com o método PBL. A abordagem metodológica é um estudo de caso analisado microetnograficamente. Através das rubricas desenhadas contextualmente, foi possível acompanhar e avaliar o desempenho dos alunos de forma holística, bem como o seu engajamento dentro do processo de resolução de problemas, mostrando-se um meio eficaz e equânime para observar as habilidades discentes requeridas nessa abordagem metodológica.
Chronic Care Model Based Nursing Interventions Improve Hypertension Patient’s Medication Compliance by Preventing Patients Forget and Fear
Authors: Proboningsih Jujuk , Sriyono Sriyono , Najib Mohammad , Fitriah Fitriah
Published: February 2023

The goal of nursing intervention on chronic disease such as Hypertension is to improve the patient self-care, in which the Chronic care model could help to reach the best outcome. Research suggests the community, health workers, and regulations could improve the health. The purpose of this study was to apply nursing intervention based on chronic care model to medication adherence in hypertensive patients. This is quasi-experimental research used pre-post test control group design. 50 respondents of hypertension patients were recruited from Pucang Sewu, Pacar Keling and Tambak Rejo Surabaya Health Centres. The intervention was applicating nursing interventions based on the Chronic Care Model. The Mann Whitney U statistical analysis used to understand the effect of the intervention to the medication adherence in patients with hypertension. A total of 50 respondents who were divided into two groups, control and treatment, with each (n = 25). The distribution of characteristics in the two groups is homogeneous. Different test in the control group, there was no difference. The treatment group shown a significant difference in adherence before and after the intervention with p value 0.000 (p <0.05). This study found the reason of disobedient patient to medication, which is forget and fear to the side effect. The patient and the health care agree frequent reminder could be the solution to this problem. Chronic Care Model based nursing interventions can improve medication adherence in hypertensive patients by encourage the health care system to frequently remind patients.
Improving Oral Hygiene Maintenance Through Teacher Empowerment to Reduce the Debris Index of Children with Disabilities
Authors: Prasetyowati Silvia , Isnanto Isnanto , Pipit R Aprilinda
Published: February 2023

The prevalence of disability continues to increase. In 2003 there were 0.9% of all children in Indonesia with disabilities, in 2006 there were 1.38% of all children in Indonesia with disabilities and the peak in 2012 was 2.45% of all children in Indonesia. Children with disabilities are vulnerable and prone to exposure to diseases and threats. According to the 2011 Social Protection Programme (PPLS), there were 32,990 children with disabilities from poor families. Disability is defined as the physical inability of the body to perform normal bodily functions. Teacher empowerment is very important because through teacher empowerment, teachers will gain new knowledge, new ideas in achieving the learning process. Through teacher empowerment activities on the importance of maintaining oral hygiene in students with disabilities will motivate teachers to further increase the importance of maintaining oral hygiene in students with disabilities. This study is to analyse the effect of improving oral hygiene maintenance through teacher empowerment on reducing the debris index of children with disabilities. The type of research used is Analytical Quasi Experiment. The targets in the study were all SLB D/YPAC teachers. Data collection methods using questionnaires and observation. Data analysis technique using chi square  statistical test. The  results of the  study showed that there was an effect of knowledge  and attitude of maintaining oral hygiene through teacher empowerment on reducing the debris index of children in SLB-D YPAC Surabaya.
The Prevalence and Association of Stress with Sleep Quality during the COVID-19 Pandemic among Medical Students at Airlangga University
Authors: Nugraha Adhitya Rizky , Fidiana Fidiana , Khairina Khairina , Islamiyah Wardah Rahmatul
Published: February 2023

The COVID-19 pandemic requires students to adapt to circumstances and cause various kinds of psychological problems during a pandemic outbreak. Some may experience stress, insomnia or hypersomnia. There are many factors causing poor sleep quality in students, including psychological problems such as stress which can worsen the quality of student’s sleep during the COVID-19 pandemic. This study aims to prove that there is a relationship between stress and the sleep quality of medical students at Airlangga University during the COVID-19 pandemic. This study is an observational analytic study using the cross-sectional design to assess the relationship between stress and sleep quality of respondents using the Pittsburgh Sleep Quality Index (PSQI) and Depression, Anxiety, Stress Scale - 42 (DASS-42) questionnaires. The research subjects were active pre-clinical students in the medical study program, Faculty of Medicine, Airlangga University, totaling 280 students who were divided into 3 batches, namely Class of 2019, Class of 2020, and Class of 2021. From the results of the study, it was found that 84.7% of students experienced poor sleep quality and a normal stress level of 55.5%. Sleep quality and stress levels are related to each other. This is proven by the Spearman rank analysis, which shows sleep quality is related to the stress level with a p-value = 0.000 and rho = 0.230. Based on these results, it was concluded that sleep quality and stress level have a positive correlation. The researcher expects that the institution will be able to use the study's findings as information about student stress levels and sleep quality in order to prevent stress and poor sleep or to implement health-related stress management programs for students.
Noi mi...? A volte sì!
Authors: D'Achille Paolo , De Santis Cristiana
Published: February 2023

Ci sono pervenute varie domande che chiedono se sono corrette o possibili in italiano frasi come noi mi sembriamo…, noi mi ricordiamo…, noi mi amiamo, noi mi piacciamo.
CONSUMER PERCEPTION AND SATISFACTION TOWARDS DIGITAL WALLET PAYMENTS WITH
SPECIAL REFERENCE TO PAYTM
Authors: M H N Badhusha
Published: February 2023

Paytm is the most popular app for online recharges and bill payments. This digital wallet and unified payment interface
(UPI) app offers numerous services on its platform which include payments, money transfer. Shopping, recharge
facilities, bill payments and movie, bus and air tickets. Paytm made its means into the e-commerce marketplace in the
year 2014. Now it offers multiple products ranging from mobile recharges to buying apparels or electronics.The main
objectives of this study is to evaluate the consumer perception towards Paytm, To study the reason influencing
consumers to use Paytm in Tiruchirappalli district,to find out the preference towards utility payment in Paytm.From the
data Analysis and discovery are convenient,Ease of use,safety,versatility and fast service Satisfactory.These factors will
seriously affect Satisfaction and usage of the Paytm app.Most respondents accept Paytm as Alternatives to cash and card
transactions play an important role in supporting growth towards digital India. Provide flexible services Satisfied by
Paytm.The main reason for using this Paytm app is because of all the facilities are available under the one roof such as
recharge platform, bank fund transfer, due repayment facility,Tax payment, Fee Payment, Online shopping and so on.
Paytm is mainly used for mobile / DTH recharge purpose and this reason is because of providing the cashback offers
STUDY OF DEMOGRAPHIC FEATURES, CLINICAL CHARACTERISTICS AND RISK FACTORS IN PATIENTS OF BENIGN PAROXYSMAL POSITIONAL VERTIGO (BPPV)
Authors: Patel Prachi , Patel Drashti , Solanki Zankar , Sharma Suktara
Published: February 2023

Introduction BPPV is the most common peripheral vertiginous disorder in the community.Due to overlapping clinical
features, this condition may be misdiagnosed and erroneously treated. Clear understanding of the demographic
characteristics and clinical features may help in correct diagnosis and optimal management. Method 100 diagnosed
patients of BPPV were prospectively studied. Parameters studied and analysed were:- age group and gender,affected
ear and Semi circular canal(SCC), clinical features, various diagnostic and therapeutic manoeuvres, , associated
comorbidities, treatment efficacy and recurrence rate. Results BPPV was most commonly seen in 4th decade with a
significant female preponderence(M38/F62 ). Posterior-SCC (P-SCC) is most commonly involved(82%) followed by
Horizontal-SCC (H-SCC)(17%) and Superior-SCC (S-SCC) (01%). Low serum vitamin-D3 was most commonly
associated comorbidity(34%). 84% were primary BPPV while head trauma was the most common secondary cause .
Remission rate on repositioning manoeuvre was 73%, recurrence rate 16% and failure rate was 11%. Conclusion
Undiagnosed vertigo may significantly impacts the quality of life of a person. BPPV, in spite of being one of the
commonest cause of vertigo is often misdiagnosed. If accurately diagnosed, BPPV repositioning manoeuvres have a
high cure rate