bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluating Fertilizer Influence on Overwintering Survival and Growth of Sedum Species in a Fall-installed Green Roof
Authors: Clark Mary Jane , Zheng Youbin
Published: February 2019

Vegetation success on green roofs in northern climates is challenged by extreme weather conditions, especially in winter, and is influenced by season of installation and substrate fertility. Appropriate fertilization with phosphorus (P) and potassium (K) can reduce winter injury for some plant species. The objectives of this study were to identify both the effect of P and K fertilizer rates on Sedum spp. survival over the first winter and the response of Sedum spp. growth to fertilizer rates when applied at installation. In a fall-installed extensive green roof system, survival, growth, and visual appearance of Sedum mats in non-fertilized plots (control) were compared with plots fertilized with 16–6–13 POLYON® Homogenous NPK plus Minors 3-4 month controlled-release fertilizer at 20.0 g nitrogen (N)/m2 either alone or with additional P to total 28.8, 54.4, or 80.0 g P/m2 or K to total 32.5, 51.6, or 70.6 g K/m2. Sedum mats were installed on 8 Oct. 2010 and plants in all plots survived the winter and the next year. During the 2011 growing season, vegetative coverage was not significantly different among any individual fertilized treatments; however, vegetative coverage data combined for all fertilized treatments was larger than the control. Fertilized treatments also showed larger plant height and biomass after one year, taller S. acre and S. sexangulare inflorescences, increased leaf greenness, and higher visual appearance rankings compared with the control. For individual Sedum species, S. album showed the greatest coverage in P-fertilized treatments, and effects on S. acre and S. sexangulare were treatment-dependent. Application of a controlled-release N–P–K fertilizer, without additional P or K, can be used to encourage vegetative coverage, plant growth, leaf greenness, inflorescence height, and visual appearance in fall-installed extensive Sedum green roof systems.
Evaluation of Chilling and Heat Requirements in Japanese Apricot with Three Models
Authors: Gao Zhihong , Zhuang Weibing , Wang Liangju , Shao Jing , Luo Xiaoyan , Cai Binhua , Zhang Zhen
Published: February 2019

Estimating chilling requirements is crucial for identifying appropriate cultivars for a given site, for timing applications of dormancy-breaking chemical agents, and for predicting consequences of climate change. For temperate-zone fruit species such as japanese apricot, productivity is reduced when chilling requirements are not adequately satisfied. In our study, we obtained chilling and heat requirements for flowering of six japanese apricot cultivars, which spanned the range of flowering times in this species for three successive years. Different methods for determining chilling requirements were evaluated and compared, and correlations among chilling requirements, heat requirements, and flowering date were established. The dynamic model proved to be the best for determining the chilling requirements of japanese apricot. The results showed a range of chilling requirements ranging from 26.3 to 75.7 chill portions and a narrow range of heat requirements, from 1017.7 to 1697.3 growing degree-hours (GDH). A very high correlation (R = 0.9797) between flowering date and chilling requirements and a low correlation (R = 0.4298) between flowering date and heat requirements suggest that flowering date in japanese apricot is mainly a consequence of the chilling requirements of the different genotypes, whereas heat requirements contribute a limited effect to the variation in flowering dates. Chilling requirements and heat requirements were positively related with a low correlation coefficient (R = 0.4211).
Defense Mechanisms Involved in Disease Resistance of Grafted Vegetables
Authors: Guan Wenjing , Zhao Xin , Hassell Richard , Thies Judy
Published: February 2019

Grafting with resistant rootstocks is an effective strategy to manage a variety of soilborne diseases and root-knot nematodes in solanaceous and cucurbitaceous vegetables. In addition, improved resistance to some foliar diseases and viruses has also been reported in grafted plants. Hence, grafting technology is considered an important and innovative practice of integrated pest management and a promising alternative for soil fumigants in vegetable production. Inherent resistance within rootstocks and improved plant nutrient uptake are generally suggested as the main reasons for improved disease control in grafted vegetables. However, increasing evidence indicated that systemic defense mechanisms may also play an important role in plant defense as a result of grafting. This review analyzes current literature on the use of grafting techniques for disease management in vegetable crops, discusses potential mechanisms associated with grafting-conferred plant defense, and identifies needs for future research to promote more effective and efficient use of grafting technology to support sustainable vegetable production.
Resistance of Cucumber Cultivars to a New Strain of Cucurbit Downy Mildew
Authors: Call Adam D. , Criswell Adam D. , Wehner Todd C. , Ando Kaori , Grumet Rebecca
Published: February 2019

Downy mildew, a foliar disease caused by the oomycete Pseudoperonospora cubensis (Berk. and Curt.) Rostow, is one of the most destructive pathogens of cucurbits. From 1961 to 2003, resistant cucumber cultivars in the United States had sufficient resistance to grow a successful crop without the use of fungicides. The pathogen resurged as a major problem in 2004. Since then, the dm-1 gene has not been effective against the new strain of downy mildew, and yield losses are high without the use of fungicides. The objective of this experiment was to identify cultivars having high yield and resistance to the new downy mildew. The experiment had 86 cultivars and breeding lines (hereafter collectively referred to as cultigens) and was conducted in Clinton, NC, in 2007 and 2009, in Castle Hayne, NC, in 2008 and 2009, and in Bath, MI, in 2007 to 2009. Plots were rated weekly on a 0 to 9 scale (0 = none, 1–2 = trace, 3–4 = slight, 5–6 = moderate, 7–8 = severe, and 9 = dead). Mean ratings for downy mildew leaf damage ranged from 2.9 to 5.7 in Michigan in 2008 and 2009 and from 3.8 to 6.8 in North Carolina in 2007 to 2009. None of the cultigens tested in this study showed a high level of resistance, although differences in resistance were detected. Lines WI 2757 and M 21 and cultivar Picklet were consistently among the top resistant lines in North Carolina and Michigan. The cultivars Coolgreen, Wis. SMR 18, and Straight 8 were identified as moderately to highly susceptible. An unreleased hybrid, ‘Nun 5053 F1’, and the cultivar Cates were the top yielding lines overall. The highest yield in a single year and location was from the cultivar Cates in Clinton, NC, in 2009, with 25.6 Mg·ha−1. The best cultivars in this study were only moderately resistant and would likely require fungicide applications to achieve high yield and quality in the presence of downy mildew. Until high resistance becomes available, growers would benefit by using fungicides in combination with tolerant and moderately resistant cultigens.
Increase in Epidermal Planar Cell Density Accompanies Decreased Russeting of ‘Golden Delicious’ Apples Treated with Gibberellin A4+7
Authors: Curry Eric
Published: February 2019

A 2-year study was conducted in a ‘Golden Delicious’ (Malus ×domestica Borkh.) orchard with a high incidence of physiological fruit russeting to examine the effect of gibberellin A4+7 (GA4+7) on apple epidermal cell size. Beginning at petal fall, four sequential applications of GA4+7 (0, 15, or 30 mg·L−1) were applied to whole trees every 7–10 days with an orchard air-blast sprayer at a volume of ≈1000 L·ha−1. Fruit epidermal tissue samples were taken approximately monthly beginning 1 week after the fourth application. Tissue was treated in the laboratory with an enzyme mixture to remove cellular debris in preparation for examination using either light or scanning electron microscopy (SEM). In 2007, because russeting was insignificant, treatment differences could not be established. Moreover, transillumination microscopy did not permit accurate measurement of ‘Golden Delicious’ fruit epidermal planar cell area beyond midseason because the isolated cuticle became thick and multilayered. In 2008, however, respective treatments of 15 and 30 mg·L−1 reduced calyx end russeting by 40% and 83% and increased epidermal planar cell density by 14% and 27% as measured using SEM.
Water-efficient Urban Landscapes: Integrating Different Water Use Categorizations and Plant Types
Authors: Sun Hongyan , Kopp Kelly , Kjelgren Roger
Published: February 2019

Little research has examined water requirements of entire irrigated urban landscapes integrating different types of plants. Three landscape treatments integrating different types of plants—woody, herbaceous perennial, turf—and putative water use classifications—mesic, mixed, xeric—were grown in large drainage lysimeters. Each landscape plot was divided into woody plant, turf, and perennial hydrozones and irrigated for optimum water status over 2 years and water use measured using a water balance approach. For woody plants and herbaceous perennials, canopy cover rather than plant type or water use classification was the key determinant of water use relative to reference evapotranspiration (ETo) under well-watered conditions. For turf, monthly evapotranspiration (ETa) followed a trend linearly related to ETo. Monthly plant factors (Kp) for woody plants, perennials, and turf species under well-watered conditions in this study ranged from 0.3 to 0.9, 0.2 to 0.5, and 0.5 to 1.2, respectively. Adjusted Kp for each hydrozone was calculated based on landscaped area covered by plant types as a percent of total area, and landscape factor (Kl) was calculated based on adjusted Kp for each landscape treatment. Overall, Kl relative to ETo ranged from 0.6 to 0.8 for three water use classifications.
Effects of FeEDDS and EDDS on Peat-based Substrate pH and Cu, Fe, Mn, and Zn Solubility
Authors: Albano Joseph P.
Published: February 2019

Common chelating agents used in horticultural fertilizers like ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTPA), and ethylenediaminedi(o-hydroxyphenylacetic) acid (EDDHA) are not readily biodegradable and may persist in the environment, maintaining the capacity to solubilize heavy metals. For this reason, biodegradable chelating agents like ethylenediaminedisuccinic acid (EDDS) are being evaluated for use in horticultural crop production. Therefore, the objectives of the study were to determine the effects of FeEDDS and EDDS on substrate pH and copper (Cu), iron (Fe), manganese (Mn), and zinc (Zn) solubility in peat-based substrate compared with various Fe and chelate-ligand sources. Extractions were performed using the 1:2 by volume substrate analysis method with an incubation period of 24 hours. The control was distilled deionized water extractions. Iron-source (FS) extractants consisted of 1 mg·L−1 Fe solutions derived from FeEDDS, FeEDTA, FeDTPA, FeEDDHA, and FeSO4. Iron-source extractant solution pH ranged from 7.1 (FeEDDS) to 5.4 (FeSO4). The extract pH for all Fe-source treatments was similar at pH 6.7, demonstrating the buffering capacity of the peat-based substrate. Iron recovery rates for FS treatments were determined after subtracting Fe that was freely extracted with distilled-deionized water: FeSO4 (13%), FeEDDHA (68%), FeEDDS (73%), FeEDTA (102%), and FeDTPA (121%). Iron-source treatments were not different for Mn, averaging 0.03 mg·L−1, and Cu (0.04 mg·L−1) and Zn (0.24 mg·L−1) were greatest in the FeEDDS treatment. Chelate-ligand (CL) extractants consisted of 5 mm solutions of EDDS, EDTA, and DTPA. Chelate-ligand extractant solution pH ranged from 9.7 (EDDS) to 2.3 (DTPA), and extract solution pH ranged from 7.2 (EDDS) to 4.7 (DTPA). Extractant solutions of EDDS and DTPA resulted in the lowest and highest levels of Cu (0.06 and 0.14 mg·L−1, respectively) and Fe (4.3 and 13.1 mg·L−1, respectively) in extract solutions. Overall, these results suggest that there are no negative implications for the use of FeEDDS with peat-based substrate in terms of horticultural crop production based on substrate Fe solubility, which was not different from FeEDTA.
Recessive Resistance to Tomato yellow leaf curl virus from the Tomato Cultivar Tyking Is Located in the Same Region as Ty-5 on Chromosome 4
Authors: Hutton Samuel F. , Scott Jay W. , Schuster David J.
Published: February 2019

Tomato yellow leaf curl virus (TYLCV), a Begomovirus in the family Geminiviridae, is an important disease of cultivated tomato (Solanum lycopersicum L.) in many parts of the world. Disease is managed primarily by chemical control of the vector, the sweetpotato whitefly, Bemisia tabaci (Genn.), and by growing resistant varieties. Resistance derived from the cultivar Tyking is being used in many breeding programs, but the location of resistance factors has not been reported. The breeding lines Fla. 8753 and Fla. 344 both have high levels of resistance to TYLCV derived from ‘Tyking’ and from S. chilense accession LA 1938, but none of their parent lines contain any of the known genes Ty-1 to Ty-4. An additional resistance locus, Ty-5, was recently identified, and to determine if this locus controls TYLCV resistance in Fla. 8753 and Fla. 344, appropriate segregating populations were analyzed using the Ty-5 marker, SlNAC1. Results show that SlNAC1 cosegregates with a recessive allele derived from ‘Tyking’. We suggest the gene symbol ty-5 be used to describe this gene. Mean disease severity of progeny homozygous for either the resistant or susceptible alleles did not equal parental levels of resistance and susceptibility, respectively, suggesting the involvement of an additional gene that is likely derived from LA1938.
Drip Irrigation in California Strawberry Nurseries to Reduce the Incidence of Colletotrichum acutatum in Fruit Production
Authors: Daugovish Oleg , Bolda Mark , Kaur Sukhwinder , Mochizuki Maren J. , Marcum Daniel , Epstein Lynn
Published: February 2019

Strawberry anthracnose caused by Colletotrichum acutatum is often asymptomatic on plants in sprinkler-irrigated nurseries but destructive after transplantation into fruiting fields. This study evaluated the impact of strawberry nursery sprinkler and drip irrigation in the presence or absence of C. acutatum on post-transplantation plant growth, mortality, and fruit yield in fruiting fields in California. In a 2005 nursery at Tulelake, CA, dip infestation of mother plants with C. acutatum reduced early-season mother plant canopy size by 21% and delayed runner production in mother plants but otherwise had no obvious disease symptoms. In comparison with sprinkler-irrigated treatments in the nursery, drip irrigation of infested nursery plots reduced plant losses in fruit production fields by 86% at Watsonville, CA, in 2005 and 50% and 75% at Oxnard, CA, in 2005 and 2008. Transplants from infested nursery treatments had a 33% to 60% smaller canopy and 11% to 42% lower yield than transplants from uninfested nursery treatments. However, transplant canopy size and yield from the infested and then drip-irrigated nursery treatment were similar to the sprinkler-irrigated, non-infested nursery treatment. Quantitative polymerase chain reaction measurements of C. acutatum in crown tissue of fruiting field plants seven weeks after transplanting showed significantly more C. acutatum (≈11×) in their crowns in sprinkler-irrigated than drip-irrigated, infested nursery transplant treatments. During the course of fruit production, the amount of C. acutatum in crown tissue increased in all treatments. However, at the end of the fruit season, there was still significantly more (≈8×) C. acutatum in the crowns of the plants produced by sprinkler irrigation than by drip irrigation in the nursery. These data suggest that if C. acutatum is present in the nursery, drip irrigation can reduce subsequent plant stunting and yield losses in strawberry production fields.
Pollen Morphology, Viability, and Germination of Tanacetum vulgare L.
Authors: Devrnja Nina , Milojević Jelena , Tubić Ljiljana , Zdravković-Korać Snežana , Cingel Aleksandar , Ćalić Dušica
Published: February 2019

The pollen morphology of aromatic Tanacetum vulgare L. (Asteraceae), which has wide horticultural and medicinal uses, was examined using light microscopy and scanning electron microscopy. The investigation revealed that pollen grains are radially symmetrical, isopolar, spheroidal, tricolporate, and echinate–perforate. Average pollen length was 21.32 ± 1.12 μm, whereas average pollen width was 20.04 ± 0.98 μm (length:width ratio 0.94). Spine length was 2.72 ± 0.29 μm. Average distance from the spines was 7.15 ± 0.31 μm. Pollen exine consisted of 1–3 pores. Pori are elongated and also with a distinct margin. The porus latitude is smaller than the colpus latitude. One to three perforations were noticed on 1 μm2 of exine. Fluorescein diacetate was used to assess the viability of T. vulgare pollen. The effect of sucrose (1%, 5%, 10%, 20%, and 30%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of sucrose in the medium improved both pollen germination and tube growth. Also, pollen nucleus status was determined. Binucleate and trinucleate mature pollens were observed. Overall, the palynological features of this species may be helpful for further taxonomical and pharmaceutical investigations.
Relationship Between the Levels of Non-structural Carbohydrates, Digging Date, Nursery-growing Environment, and Chilling in Strawberry Transplants in a Subtropical Environment
Authors: Menzel Christopher M. , Smith Lindsay
Published: February 2019

Experiments were conducted to study the effect of time of digging and nursery-growing environment on the levels of non-structural carbohydrates in ‘Festival’ strawberry transplants (Fragaria ×ananassa) over 2 years in southeastern Queensland, Australia. We were interested in determining whether there was a strong relationship between the potential productivity of this material and reserves in the plants. First, bare-rooted plants were obtained from Stanthorpe in southern Queensland from early March to mid-April/late April. Second, bare-rooted plants were sourced from Stanthorpe (a warm-growing area) or from Toolangi in Victoria (a cool-growing area). In Year 1 of the experiments, the nursery material from the different treatments was grown at Nambour in southeastern Queensland and fruit yield determined. The total weight of non-structural carbohydrates/plant increased as digging was delayed and was higher in the plants from Stanthorpe than the plants from Toolangi. Plants dug on 17 Mar. in Year 1 had higher weights of non-structural carbohydrates [292 mg/plant dry weight (DW)] than plants dug on 3 Mar. (224 mg/plant) and higher early yield to the end of June or to the end of July and higher total yield to mid-October adjusted by the length of the growing season for the different treatments. Plants dug on 1 Apr. (408 mg/plant) or on 13 Apr. (445 mg/plant) had higher reserves than the plants dug on 17 Mar. but lower yields. Only the differences in yields between the plants dug on 3 Mar. and 17 Mar. reflected the differences in carbohydrates. The stock from Stanthorpe had greater reserves (408 mg/plant) than the stock from Toolangi (306 mg/plant) but similar yields in Year 1 possibly because of poorer flowering in the nursery plants. It was concluded that carbohydrate reserves in transplants only partially reflect their productivity in this environment.
Identification and Mapping of DNA Markers Linked to Eastern Filbert Blight Resistance from OSU 408.040 Hazelnut
Authors: Sathuvalli Vidyasagar R. , Mehlenbacher Shawn A. , Smith David C.
Published: February 2019

Eastern filbert blight (EFB) of European hazelnut (Corylus avellana L.), caused by the pyrenomycete Anisogramma anomala (Peck) E. Müller, is a major disease problem and production constraint in Oregon’s Willamette Valley. Host genetic resistance is viewed as the most economical means of controlling this disease. Marker-assisted selection has been extensively used for ‘Gasaway’ resistance in the hazelnut breeding program at Oregon State University (OSU). Concern over potential breakdown of this single resistance gene prompted a search for new sources of resistance. Selection OSU 408.040 showed no signs or symptoms of the fungus after a series of disease inoculations, and resistance was transmitted to half of its offspring, indicating control by a dominant allele at a single locus. In this study, we identified six random amplified polymorphic DNA (RAPD) and 11 simple sequence repeat (SSR) markers linked to EFB resistance from OSU 408.040. The new markers supplement the previously identified amplified fragment length polymorphism (AFLP) markers. A linkage map constructed in the progeny OSU 245.098 × OSU 408.040 spanned a distance of 19.5 cM with the resistance locus cosegregating with AFLP marker A8-150 and located between SSR markers LG675 and LG682. Using SSR markers as anchor loci, OSU 408.040 resistance was assigned to linkage group 6 (LG6). Comparison with the previously mapped ‘Gasaway’ resistance locus showed that resistance from OSU 408.040 maps to the same location.
Evaluation of Grafting Using Hybrid Rootstocks for Management of Bacterial Wilt in Field Tomato Production
Authors: McAvoy Theodore , Freeman Joshua H. , Rideout Steven L. , Olson Stephen M. , Paret Mathews L.
Published: February 2019

Seven hybrid tomato rootstocks with possible resistance to bacterial wilt caused by Ralstonia solanacearum and a known resistant cultivar were tested as grafting rootstocks to impart resistance to a bacterial wilt-susceptible cultivar, BHN 602. Greenhouse studies showed resistance of all the rootstocks to bacterial wilt. The disease incidence and yield of ‘BHN 602’ grafted to these rootstocks were evaluated in open-field tomato production in Florida and Virginia over four seasons. Significant differences in bacterial wilt incidence were observed between grafted entries in three of the four trials. In these three trials, grafted entries consistently exhibited the least bacterial wilt incidence compared with the controls; the self-graft, and non-grafted entries. Over all the trials, tomato plants grafted onto ‘Cheong Gang’, ‘BHN 1054’, and ‘BHN 998’ displayed the least bacterial wilt incidence. Rootstocks had a significant effect on total marketable yield in all the trials with certain grafted entries yielding significantly greater than non-grafted ‘BHN 602’. Field studies show that grafting holds promise for decreasing the impact of bacterial wilt on tomato cultivars as well as increasing the overall productivity of tomato cultivars.
Effect of Distillation Time on Mentha canadensis Essential Oil Yield and Composition
Authors: Zheljazkov Valtcho D. , Astatkie Tess
Published: February 2019

Japanese cornmint (Mentha canadensis L.) is a major essential oil crop grown in Asia, South America, and to a limited extent in eastern Europe. Japanese cornmint oil is the only commercially viable source for crystalline menthol. We hypothesized that the length of the distillation time (DT) will have an effect on Japanese cornmint essential oil content and composition. Therefore, the objective was to evaluate the effect of eight DTs (1.25, 2.5, 5, 10, 20, 40, 80, and 160 min) on essential oil content and composition. The essential oil content (0.43% to 1.06% range) reached maximum at 10 min DT; further increase in DT did not significantly increase essential oil content. The concentrations of alpha-pinene (0.14% to 0.76% range), beta-pinene (0.23–0.81), 3-octanal (0.19–0.34), limonene (0.69% to 1.53%), eucalyptol (0.06% to 0.12%), isopulegone (0.42% to 0.56%), and isomenthone (4.4% to 5.7%) were highest at 1.25 to 5 min DT and generally decreased to their respective minimums at 160 min DT. The concentration of menthone (4.3% to 6.3%) was highest at 1.25 min DT, decreased at 2.5 min, and was lowest at 10 to 160 min DT. The concentration of piperitone (0.98% to 1.27%) was lowest at 1.25 min DT and higher at 5 to 40 min DT compared with other DTs. The concentration of menthol (74% to 79%) was low at 1.25 min, then increased at 10 to 80 min DT, and reached a maximum at 160 min DT. Generally, the yield of most individual essential oil constituents was lower at 1.25 min DT relative to the other DT and reached maximum at 10 to 20 min DT. The yield of menthol was low at 1.25 to 2.5 min DT and rose at 5 min to 160 min DT. The results suggest that different DTs can be used to maximize recovery of certain constituents. Also, the results demonstrated that there are no oil yield gains after 20 min DT, which is much lower than the usual distillation time of 60 min or more. This study can be used as a reference when comparing reports in which different DTs were used.
Symbiogenics: An Epigenetic Approach to Mitigating Impacts of Climate Change on Plants
Authors: Woodward Claire , Hansen Lee , Beckwith Fleur , Redman Regina S. , Rodriguez Rusty J.
Published: February 2019

We have found that plants in natural ecosystems adapt to abiotic stress by forming symbiotic associations with Class 2 fungal endophytes. Without the endophytes, plants are not stress-tolerant and do not survive in the habitats to which they are adapted. Symbiotically conferred stress tolerance typically occurs in a habitat-specific manner and is based on interactions between environmental factors and both plant and fungal genomes. For example, endophytes from geothermal plants confer heat tolerance, and endophytes from coastal plants confer salt tolerance. We have designated this phenomenon as habitat-adapted symbiosis and hypothesize that it is a ubiquitous aspect of plant ecology. Class 2 endophytes also increase plant growth and development while decreasing water consumption. We present metabolic and gene expression data to support a working model for the underlying mechanisms of endophyte-conferred benefits to plants. Although endophytes had no effect on photosynthetic rate and metabolic efficiency, they significantly increased photosynthetic efficiency. Endophytes significantly altered the ratio of upregulated to downregulated (UR:DR genes) plant genes compared with nonsymbiotic plants. Specific UR:DR gene ratios varied with endophyte species as well as habitat of origin. Collectively, these observations have allowed us to design a new symbiogenic (symbio = symbiosis; genic = gene influence) strategy for mitigating impacts of climate change on crop production.
Morphological and Genetic Variation among Four High Desert Sphaeralcea Species
Authors: Sriladda Chalita , Kratsch Heidi A. , Larson Steven R. , Kjelgren Roger K.
Published: February 2019

The herbaceous perennial species in the genus Sphaeralcea have desirable drought tolerance and aesthetics with potential for low-water use landscapes in the Intermountain West. However, taxonomy of these species is ambiguous, which leads to decreased consumer confidence in the native plant nursery industry. The goal of this study was to test and clarify morphological and genetic differentiation among four putative Sphaeralcea species. Morphological characteristics of the type specimens were used as species references in canonical variate analysis to generate a classification model. This model was then used to assign putative species names to herbarium voucher specimens and to field-collected voucher specimens to clarify genetic variation among species. Field specimens were also classified using Bayesian cluster analyses of amplified fragment length polymorphism (AFLP) genotypes. Sphaeralcea coccinea (Nutt.) Rydb. and S. grossulariifolia (Hook. & Arn.) Rydb. formed a composite group morphologically and genetically distinct from the S. munroana (Douglas) Spach and S. parvifolia A. Nelson composite group. Each composite group displayed genetic isolation by geographic distance. Also, morphological traits of S. munroana and S. parvifolia correlated to geographic distance. Taken together these results suggest that our samples represent two sympatric yet reproductively isolated groups. Distinguishing between these two Sphaeralcea composite groups can create greater consumer confidence in plant material developed for use in Intermountain West low-water landscaping.
A Multistaged Fuzzy Logic Scheme in a Biobotanic Growth Regulation System
Authors: Chang Chung-Liang , Sie Ming-Fong
Published: February 2019

A multistaged fuzzy logic control method was used in the development of a bionic botanical growth control system. The growth mode combined fuzzy logic inference with expert knowledge to regulate the growth rate of plants. First, environment factors such as the light, temperature, and water required for plants in different stages of growth were analyzed. Fuzzy logic was then used to establish membership functions, an inference engine, and rule table. An expert database related to plant growth was combined with the fuzzy logic controller to formulate a plant growth control system. Sunflowers were used as a simulated model and the results correspond to the information provided by experts. The proposed model was used to control the growth rate of plants based on data provided in the expert database. The proposed method and results of this study are applicable in the management and control of environments for the growth of crops.
Indigenous Crops of Asia and Southeast Asia: Exploring Health-promoting Properties
Authors: Patil Bhimanagouda S. , Jayaprakasha G.K. , Vikram Amit
Published: February 2019

Diets rich in vegetables and fruits are known to be protective against several diseases. Only a limited number of vegetables and fruits are consumed as part of the daily diet in Western countries. Historically, indigenous vegetables and fruits are known for their medicinal and nutritional value in countries where they were originated and/or domesticated. However, relatively few systemic studies and reviews were conducted to enumerate the potential of these vegetables to human health benefits. Although certain indigenous crops have received attention, the majority of these crops with strong potential biological activities were neglected and/or not reported. Considering the current health-related problems and obesity-related diseases, it is timely to enumerate the health-promoting properties of certain indigenous vegetables. In this report, we have reviewed some of the important crops indigenous to Southeast Asia and their potential health-promoting properties.
The Future Potential of Horticultural Plant Discovery, Improvement, and Production in Nepal
Authors: Schnelle Michael A.
Published: February 2019

The country of Nepal is geographically variable and thus has significant diversity in its native flora. Because of physical and social barriers that still exist, many indigenous plant materials have yet to be adequately screened for their uses not only within Nepal, but outside its borders. Maximum production of horticultural crops in Nepal will require improved water distribution, adequate pest control, and consideration of social/demographic issues.
Stem Anatomy and Grafting Success of Chinese Chestnut Scions on ‘AU-Cropper’ and ‘Qing’ Seedling Rootstocks
Authors: Warmund Michele R. , Cumbie Billy G. , Coggeshall Mark V.
Published: February 2019

Grafting failure of Chinese chestnut (Castanea mollissima Blume) trees is common. Some researchers have suggested that secondary phloem fibers in chestnut rootstock stems may interfere with graft union formation. Others suggest that high rootstock moisture content may reduce grafting success. This study was conducted to ascertain the precise arrangement of secondary phloem fibers in Chinese chestnut seedling rootstocks and to determine if they are associated with grafting failure. We also investigated the effects of moisture content of rootstocks and container growing medium on grafting success. Nodes on Chinese chestnut seedlings had 2/5 phyllotaxy with five alternate buds present in two revolutions around the stem. Vascular cambium in 1-year-old rootstock stems was arranged in a regular five-lobed cylinder in cross-sections. Primary phloem fibers were present in a discontinuous ring and secondary phloem fibers were usually clustered outside the cambium between the lobes. Grafts were successful when scion buds were placed on the rootstock disrupting secondary phloem fibers with cambia of the budded tissues aligned. Containerized rootstocks at 56% and 62% growing medium moisture content before grafting had 25% graft union success rates, whereas non-irrigated plants at 48% moisture had a 75% success rate.
Estimating Midday Leaf and Stem Water Potentials of Mature Pecan Trees from Soil Water Content and Climatic Parameters
Authors: Deb Sanjit K. , Shukla Manoj K. , Mexal John G.
Published: February 2019

Diurnal and temporal patterns of stem water potential (ψstem) and leaf water potential (ψleaf) were determined during June to Sept. 2010 and 2011 at lower (2.5 m tree height), mid- (4.6 m), and upper (7.6 m) canopy positions for two flood-irrigated, mature pecan [Carya illinoinensis (Wangenh.) K. Koch] orchards near Las Cruces, NM. Diurnal measurements of ψstem and ψleaf at three canopy heights were correlated under both dry and wet soil conditions. However, although soil water contents at Site 2 (silty clay loam texture) remained higher compared with Site 1 (sandy loam), ψstem and ψleaf values, particularly under dry soil conditions at Site 2, were consistently lower, showing the effect of clayey soil texture on pecan water stress. Diurnal patterns of ψstem and ψleaf indicated that measurements of ψstem and ψleaf should be made close to early afternoon (between 1400 and 1500 hr Mountain Standard Time) to evaluate mature pecan water stress, which also corresponded to maximum climatic stress conditions. Midday ψstem and ψleaf measured at three canopy heights over several irrigation cycles during the 2010 season were correlated with one another, midday soil water content at different depths, and atmospheric vapor pressure deficit (VPD). Multiple regression analysis [between midday ψstem or ψleaf and midday θavg (soil water content at 0 to 40 cm), air temperature (Tmd), and relative humidity (RHmd)] during the 2010 season revealed that two-parameter regression models [ψstem or ψleaf = f (midday θavg and Tmd)] were the most significant for the interpretation of midday ψstem or ψleaf at both sites. Using the two-parameter model, predictions of ψstem and ψleaf measured on the both shaded and sunlit sides of trees at three canopy heights for 2011 showed good agreement between measured and predicted ψstem and ψleaf (R2 ranged from 0.70 to 0.98). Two-parameter models derived in an earlier study generally underpredicted ψstem both in 2010 and 2011, which further supported the importance of the time of midday ψstem and ψleaf measurements suggested in this study.
Pecan Tree Productivity, Fruit Quality, and Nutrient Element Status using Clover and Poultry Litter as Alternative Nitrogen Fertilizer Sources
Authors: Wells M. Lenny
Published: February 2019

The recent increase in the cost of synthetic fertilizer dramatically reduces the profit margin for pecan [Carya illinoinensis (Wangenh.) K. Koch] producers. The objective of this study was to investigate the effects of clover and poultry litter on the orchard soil, horticultural, and nut quality parameters of pecan in the southeastern United States. The following treatments were evaluated; 1) crimson clover (Trifolium incarnatum L.); 2) poultry litter; 3) crimson clover + poultry litter; 4) ammonium nitrate (NH4NO3); and 5) untreated control. Application of poultry litter with or without clover often led to higher soil phosphorous (P) and potassium (K). Poultry litter application with and without clover led to higher leaf P in the final year of study. The recurring low pecan leaf K in the presence of clover without additional K application suggests that K nutrition may be especially important in orchards where clover is used. Clover and/or clover + litter occasionally led to enhanced pecan leaf concentrations of iron (Fe), copper (Cu), and zinc (Zn). Over the course of the study, yields were more consistent from year to year in the clover, litter, and clover + litter treatments, as indicated by the low alternate bearing intensity (I) from 2008 to 2011. Leaf elemental tissue analysis, pecan yield, and quality indicate that poultry litter and clover provide adequate nitrogen (N) nutrition for pecan production.
In Situ Measurement of Pecan Leaf Nitrogen Concentration using a Chlorophyll Meter and Vis-near Infrared Multispectral Camera
Authors: Hardin James A. , Smith Michael W. , Weckler Paul R. , Cheary Becky S.
Published: February 2019

Knowledge of foliar nitrogen (N) concentration is important in pecan [Carya illinoinensis (Wang.) K. Koch] management protocols. Lower cost and/or rapid methods to determine foliar N are desirable and may result in improved management strategies as well as enable precision agricultural practices to be deployed in pecan production. This study investigates using a portable chlorophyll meter and Vis-NIR camera to rapidly determine pecan foliar N in situ. Relationships of SPAD values from a chlorophyll meter (Minolta SPAD 502Plus) and vegetative indices calculated from camera image data to foliar N determined by chemical analysis were investigated. SPAD readings were taken monthly from May through October on ‘Pawnee’, ‘Kanza’, and ‘Maramec’ pecan cultivars in Oklahoma in 2010. Images of the same ‘Pawnee’ and ‘Kanza’ trees were collected in September and October of 2010 with a truck-mounted multispectral camera using ambient light. Correlation of foliar N to SPAD values was poor in May for all cultivars but distinct significant linear relationships were found for ‘Maramec’ and ‘Pawnee’ for each of the other months tested with R2 ranging from 0.40 to 0.87. Data from ‘Kanza’ had significant relationships in June and October with R2 of 0.39 and 0.72, respectively. Normalized difference vegetative index (NDVI) and reflectance data extracted from Vis-NIR camera images were significantly correlated to foliar N in both months of the study on ‘Pawnee’ but only in September for ‘Kanza’. The various relationships had R2 between 0.21 and 0.51.
Cultural and Environmental Factors Governing Tomato Production: Local Production under Elevated Temperatures
Authors: Snider John L. , Russo Vincent M. , Roberts Warren , Wann Elbert V. , Raper Randy L.
Published: February 2019

Long-term fresh tomato (Solanum lycopersicum L.) production data were used to estimate cultural and environmental impacts on marketable tomato yields in eastern Oklahoma. Quantifying the interactive effects of planting date and growing season duration and the effects of cumulative heat units and heat unit accumulation rate on marketable yields allowed for productivity estimates based on past temperature conditions. Simulated increases in air temperature were predicted to reduce yields and increase the amount of cropland needed to meet local consumption demands. Consequently, local tomato production in Oklahoma may be negatively impacted under elevated temperature conditions projected under global climate change.
Heterogeneous Consumer Preferences for Native and Invasive Plants: Evidence from Experimental Auctions
Authors: Yue Chengyan , Hurley Terry , Anderson Neil O.
Published: February 2019

All stakeholders along the supply chain affect the dispersal of native and invasive horticultural plants. This is especially true for the consumers who determine how the plants are ultimately used. Therefore, consumer attitudes toward native and invasive plants cannot be ignored. This study used an experimental auction to explore market segmentation among consumers in terms of their preference and willingness to pay for labeled native and invasive attributes. We identified three market segments, namely, “nativists” (16%), “invasive averse” (34%), and “typical” (50%) consumers. The three segments of consumers differed in their demographics and attitudes toward native and invasive attributes. From a government policy perspective, labeling invasive or native plants could potentially change the behavior of some consumers, but half of the market is unlikely to be substantially swayed by invasive/native labeling. Therefore, supply-side intervention policies such as sales restrictions may be more effective at promoting native plant purchases and restricting the purchase and spread of invasive plants.
A New Look at Farmers’ Markets: Consumer Knowledge and Loyalty
Authors: Gao Zhifeng , Swisher Marilyn , Zhao Xin
Published: February 2019

Farmers’ markets (FMs) are perceived as ideal places for consumers to purchase fresh, local, and organic produce; for small- and midsized farmers, to gain reliable income; and for stimulating the local economy. However, with the organic and local food movements gaining momentum, it may be hard to keep up with all the expectations for FMs. This is because the rapid growth of FMs may provide more opportunities for vendors who use misleading labels and statements to attract consumers. The objective of this study was to determine consumer perception and knowledge of FMs as well as consumer persistence of shopping at FMs after finding out that the FM products do not meet their expectations. The results indicated that FM shopping atmosphere, environmental consciousness, product freshness, and local production were the main reasons for consumers shopping at FMs. This study showed that the majority of consumers had limited knowledge of individual FM vendors; most consumers would continue to shop at FMs even after purchasing products that did not meet their expectations; and consumers who believed buying locally at FMs was important were more likely to stop shopping if dishonest vendor practices were revealed than were consumers who used FMs mainly as places for socializing and meeting friends.
Optimization of In Vitro Regeneration from Leaf Explants of Apple Cultivars Golden Delicious and Melrose
Authors: Mitić Nevena , Stanišić Mariana , Milojević Jelena , Tubić Ljiljana , Ćosić Tatjana , Nikolić Radomirka , Ninković Slavica , Miletić Rade
Published: February 2019

An efficient in vitro shoot regeneration method from leaf explants of apple cultivars Golden Delicious and Melrose by optimization of regeneration medium, explant type and orientation, dark pre-treatment, and gelling agent is presented. Murashige and Skoog’s regeneration medium containing 22 μM thidiazuron (TDZ) and 1.5 μM indole-3-butyric acid (IBA) (M2 medium) was superior for regeneration as well as for subsequent shoot multiplication in both cultivars, providing regeneration frequency of 95% or higher in the best combination with other factors. Pre-incubation in the dark proved to be an essential factor for regeneration. The use of agar as a gelling agent provides satisfactory regeneration frequency compared with media gelled with PhytagelTM. Leaf explants of cv. Melrose with adaxial surface in contact with M2 medium and those of cv. Golden Delicious orientated contrary regenerated the highest mean number of shoots per explant. Under optimal conditions, a maximal index of shoot-forming capacity of 11.44 and 6.30 for ‘Melrose’ and ‘Golden Delicious’, respectively, was achieved. Regenerated shoots were successfully rooted and acclimated ex vitro.
Evaluation of Water–Air Balance of Various Substrates on Begonia Growth
Authors: Londra Paraskevi A. , Paraskevopoulou Angeliki T. , Psychoyou Maria
Published: February 2019

The water–air balance of four soilless substrates: 75% sphagnum peat–25% perlite (Ps75:P25), 50% sphagnum peat–50% perlite (Ps50:P50), 50% coir–50% perlite (C50:P50), and a fortified substrate with 60% sphagnum peat–30% black peat–10% perlite (Ps60:Pb30:P10) (in a volumetric proportion) was investigated under two different irrigation methods (drip and sub-irrigation), and its effect on the growth of Begonia ×elatior ‘The President’ was studied. The bulk density, particle size distribution, and water retention curve of the substrates were determined. Furthermore, the water profiles, oxygen (Ο2) concentration, and Ο2 diffusion rate of all substrates were determined during a 16-week cultivation period. Plant height, flower production, and both shoot and root dry weights as well as percent growth increase of plants were measured at the end of the experiment. The substrate water profiles showed that the water content was greater and air content was less in substrates of pots irrigated with drip irrigation than with sub-irrigation. The O2 concentration in all substrates irrespective of the irrigation method was high. The O2 diffusion rate values of sub-irrigated substrates were greater than those drip-irrigated, and Ps60:Pb30:P10 showed the greatest values. Shoot and root dry weights and percent growth increase of drip-irrigated plants were greater than that of sub-irrigated plants.
Combining Preemergence Herbicides in Tank Mixtures or as Sequential Applications Provides Season-long Crabgrass Control in the Upper Midwest
Authors: Proctor Christopher A. , Sousek Matt D. , Patton Aaron J. , Weisenberger Daniel V. , Reicher Zachary J.
Published: February 2019

Preemergence (PRE) herbicides are used to control crabgrass (Digitaria spp.). Single spring applications are common in areas with relatively low crabgrass pressure, whereas sequential applications often are used to extend control in locations with high crabgrass pressure. Our objectives were to determine if changing a.i. in initial and sequential applications affects crabgrass control and if single spring applications of tank-mixed PRE herbicides provide season-long crabgrass control. Studies were conducted 2009, 2010, and 2011 in West Lafayette, IN, and 2011 in Wymore, NE. The PRE herbicides prodiamine, pendimethalin, and dithiopyr were tested using different application strategies. Sequential applications were applied mid-April and mid-June using all possible combinations of the three herbicides and untreated for the initial and sequential application. These herbicides also were applied mid-April as single full-rate PRE application or as a tank mixture of two PRE herbicides at half-plus-half or half-plus-quarter rate. Season-long crabgrass control was consistently achieved using sequential applications regardless which of the three herbicides was used for initial or sequential applications. Single applications of tank mixtures also provided consistent crabgrass control, equivalent to single full-rate applications of the individual PRE herbicides. Tank mixtures of half-plus-quarter rate and single half-rate applications resulted in more crabgrass cover than single full-rate or half-plus-half rate applications regardless of the herbicide applied.