bluuebunny/crossref_metadata_2025 huggingface dataset:
Gas Turbine Combustion Technologies for Hydrogen Blends
Authors: Cecere Donato , Giacomazzi Eugenio , Di Nardo Antonio , Calchetti Giorgio
Published: September 2023

The article reviews gas turbine combustion technologies focusing on their current ability to operate with hydrogen enriched natural gas up to 100% H2. The aim is to provide a picture of the most promising fuel-flexible and clean combustion technologies, the object of current research and development. The use of hydrogen in the gas turbine power generation sector is initially motivated, highlighting both its decarbonisation and electric grid stability objectives; moreover, the state-of-the-art of hydrogen-blend gas turbines and their 2024 and 2030 targets are reported in terms of some key performance indicators. Then, the changes in combustion characteristics due to the hydrogen enrichment of natural gas blends are briefly described, from their enhanced reactivity to their pollutant emissions. Finally, gas turbine combustion strategies, both already commercially available (mostly based on aerodynamic flame stabilisation, self-ignition, and staging) or still under development (like the micro-mixing and the exhaust gas recirculation concepts), are described.
Experimental Study on Flame Response Characteristics of a Non-Premixed Swirl Model Combustor
Authors: Yang Chen , Liu Yong , Zhang Xiang , Li Hao , Ge Xinkun , Jin Feng , Liu Chongyang
Published: September 2023

Non-premixed swirl combustion has been widely used in pieces of industrial combustion equipment such as industrial boilers, furnaces, and certain specific gas turbine combustors. In recent years, the combustion instability of non-premixed swirl flames has begun receiving attention, yet there is still a lack of related research in academia. Therefore, in this study, we conducted experimental research on a swirl stabilized gas flame model combustor and studied the heat release response characteristics of the swirl combustor through the flame transfer function. Firstly, the flame transfer function (FTF) was measured under different inlet velocities and equivalence ratios, and the experimental results showed that the FTF gain curve of the non-premixed swirl flame exhibited a significant “bimodal” shape, with the gain peaks located around 230 Hz and 330 Hz, respectively. Secondly, two oscillation modes of the flame near the two gain peaks were identified (the acoustic induced vortex mode Mv and the thermoacoustic oscillation mode Ma), which have not been reported in previous studies on swirl non-premixed flames. In addition, we comprehensively analyzed the flame pulsation characteristics under the two oscillation modes. Finally, the coupling degrees between velocity fluctuations, fuel pressure fluctuations, and heat release fluctuations were analyzed using the Rayleigh Index (RI), and it was found that in the acoustic-induced vortex mode, a complete feedback loop was not formed between the combustor and the fuel pipeline, which was the main reason for the significant difference in the pressure fluctuation amplitude near 230 Hz and 330 Hz.
Drought Exerted a Stronger Controlling Effect on Soil Carbon Release than Moisturizing in a Global Meta-Analysis
Authors: Xiao Jiamin , Lin Yonghui , He Xingbing , He Zaihua , Kong Xiangshi
Published: September 2023

The carbon cycle within a terrestrial ecosystem is a pivotal functional process that drives ecosystem evolution, and the precipitation pattern variations exert a profound influence on it. To comprehensively assess the response of carbon release in the global terrestrial ecosystem to water variation, we performed a global meta-analysis by extracting data from 144 publications. Additionally, we incorporated various moderators to elucidate the heterogeneity observed in the data. The results showed that soil carbon release was highly sensitive to water variation, with drying and moisturizing treatments responding differently to water variability. Specifically, drought inhibited the soil carbon release of terrestrial ecosystems (24% reduction in effect size), but precipitation promoted it (11% increase in effect size). Moreover, this sensitivity could be affected by other ambient factors, depending on water manipulation (drying or moisturizing treatment). In moisturizing treatment cases, ambient precipitation, altitude, and vegetation type more or less affected the sensitivity of soil carbon release to a water increase. However, in drying treatment cases, these factors had no significant influence on the water sensitivity of soil carbon release. Unlike the above ambient factors, a temperature increase strengthened this sensitivity in both of the treatments. In addition, our study also showed that the response of carbon release to water variation did not depend on the substrate type or the carbon–nitrogen ratio (C/N) of the substrates, revealing that these effect factors on carbon release on the local scale could be overshadowed by water conditions. Overall, water variation positively affected soil carbon release on the global scale. Particularly, drought had a strong controlling effect on carbon release over the other environmental factors. Therefore, the impact of soil water loss on carbon release should be of great concern for the management of ecosystems and the prediction of carbon release models, especially when high temperatures and drought have been occurring more and more frequently on the planet in recent years.
Selection of Suitable Reference Genes for RT-qPCR Gene Expression Analysis in Centipedegrass under Different Abiotic Stress
Authors: Wang Xiaoyun , Shu Xin , Su Xiaoli , Xiong Yanli , Xiong Yi , Chen Minli , Tong Qi , Ma Xiao , Zhang Jianbo , Zhao Junming
Published: September 2023

As a C4 warm-season turfgrass, centipedegrass (Eremochloa ophiuroides (Munro) Hack.) is known for its exceptional resilience to intensive maintenance practices. In this research, the most stably expressed reference genes in the leaves of centipedegrass under different stress treatments, including salt, cold, drought, aluminum (Al), and herbicide, were screened by the quantitative real-time PCR (RT-qPCR) technique. The stability of 13 candidate reference genes was evaluated by software GeNorm V3.4, NormFinder V20, BestKeeper V1.0, and ReFinder V1.0. The results of this experiment demonstrated that the expression of the UBC (ubiquitin-conjugating enzyme) remained the most stable under cold and Al stress conditions. On the other hand, the MD (malate dehydrogenase) gene exhibited the best performance in leaf tissues subjected to salt and drought stresses. Under herbicide stress, the expression level of the RIP (60S ribosomal protein L2) gene ranked the highest. The expression levels of abiotic stress-associated genes such as PIP1, PAL, COR413, ALMT9, and BAR were assessed to validate the reliability of the selected reference genes. This study provides valuable information and reference points for gene expression under abiotic stress conditions in centipedegrass.
Evaluation of Liftover Tools for the Conversion of Genome Reference Consortium Human Build 37 to Build 38 Using ClinVar Variants
Authors: Park Kyoung-Jin , Yoon Young Ahn , Park Jong-Ho
Published: September 2023

Although Genome Reference Consortium Human Build 38 (GRCh38) was released with improvement over GRCh37, it has not been widely adopted. Several liftover tools have been developed as a convenient approach for GRCh38 implementation. This study aimed to investigate the accuracy of liftover tools for genome conversion. Two Variant Call Format (VCF) files aligned to GRCh37 and GRCh38 were downloaded from ClinVar (clinvar_20221217.vcf.gz). Liftover tools such as CrossMap, NCBI Remap, and UCSC liftOver were used to convert genome coordinates from GRCh37 to GRCh38. The accuracy of CrossMap, NCBI Remap, and UCSC liftOver were 99.81% (1,567,838/1,570,748), 99.69% (1,565,953/1,570,748), and 99.99% (1,570,550/1,570,748), respectively. Variants that failed conversion via all three liftover tools were all indels/duplications: a pathogenic/likely pathogenic variant (n = 1) and benign/likely benign variants (n = 7). The eight variants that failed conversion were identified in the ALMS, TTN, CFTR, SLCO, LDLR, PCNT, MID1, and GRIA3 genes, and all the variants were not in the VCF files aligned to GRCh37. This study demonstrated that three liftover tools could successfully convert reference genomes from GRCh37 to GRCh38 in more than 99% of ClinVar variants. This study takes the first step to clinically implement GRCh38 using liftover tools. Further clinical studies are warranted to compare the performance of liftover tools and to validate re-alignment approaches in routine clinical settings.
Role of Mitochondrial Dynamics in Heart Diseases
Authors: Tokuyama Takeshi , Yanagi Shigeru
Published: September 2023

Mitochondrial dynamics, including fission and fusion processes, are essential for heart health. Mitochondria, the powerhouses of cells, maintain their integrity through continuous cycles of biogenesis, fission, fusion, and degradation. Mitochondria are relatively immobile in the adult heart, but their morphological changes due to mitochondrial morphology factors are critical for cellular functions such as energy production, organelle integrity, and stress response. Mitochondrial fusion proteins, particularly Mfn1/2 and Opa1, play multiple roles beyond their pro-fusion effects, such as endoplasmic reticulum tethering, mitophagy, cristae remodeling, and apoptosis regulation. On the other hand, the fission process, regulated by proteins such as Drp1, Fis1, Mff and MiD49/51, is essential to eliminate damaged mitochondria via mitophagy and to ensure proper cell division. In the cardiac system, dysregulation of mitochondrial dynamics has been shown to cause cardiac hypertrophy, heart failure, ischemia/reperfusion injury, and various cardiac diseases, including metabolic and inherited cardiomyopathies. In addition, mitochondrial dysfunction associated with oxidative stress has been implicated in atherosclerosis, hypertension and pulmonary hypertension. Therefore, understanding and regulating mitochondrial dynamics is a promising therapeutic tool in cardiac diseases. This review summarizes the role of mitochondrial morphology in heart diseases for each mitochondrial morphology regulatory gene, and their potential as therapeutic targets to heart diseases.
A Spanish Family with Gordon Syndrome Due to a Variant in the Acidic Motif of WNK1
Authors: Peces Ramón , Peces Carlos , Espinosa Laura , Mena Rocío , Blanco Carolina , Tenorio-Castaño Jair , Lapunzina Pablo , Nevado Julián
Published: September 2023

(1) Background: Gordon syndrome (GS) or familial hyperkalemic hypertension is caused by pathogenic variants in the genes WNK1, WNK4, KLHL3, and CUL3. Patients presented with hypertension, hyperkalemia despite average glomerular filtration rate, hyperchloremic metabolic acidosis, and suppressed plasma renin (PR) activity with normal plasma aldosterone (PA) and sometimes failure to thrive. GS is a heterogeneous genetic syndrome, ranging from severe cases in childhood to mild and sometimes asymptomatic cases in mid-adulthood. (2) Methods: We report here a sizeable Spanish family of six patients (four adults and two children) with GS. (3) Results: They carry a novel heterozygous missense variant in exon 7 of WNK1 (p.Glu630Gly). The clinical presentation in the four adults consisted of hypertension (superimposed pre-eclampsia in two cases), hyperkalemia, short stature with low body weight, and isolated hyperkalemia in both children. All patients also presented mild hyperchloremic metabolic acidosis and low PR activity with normal PA levels. Abnormal laboratory findings and hypertension were normalized by dietary salt restriction and low doses of thiazide or indapamide retard. (4) Conclusions: This is the first Spanish family with GS with a novel heterozygous missense variant in WNK1 (p.Glu630Gly) in the region containing the highly conserved acidic motif, which is showing a relatively mild phenotype, and adults diagnosed in mild adulthood. These data support the importance of missense variants in the WNK1 acidic domain in electrolyte balance/metabolism. In addition, findings in this family also suggest that indapamide retard or thiazide may be an adequate long-standing treatment for GS.
Form Poetry and the Pandemic
Authors: Mitra Tapaswinee
Published: September 2023

This article looks at a set of anglophone form poetry that I wrote for a course I took while pursuing my master’s degree in Gender Studies at Ambedkar University, Delhi (2019–2021), during the second wave of the COVID-19 pandemic in India. The poems are a documentation of the life that I lived and experienced during this time. Using an auto-ethnographic method, this article simultaneously engages with poetic forms, such as the haiku, villanelle, sestina, and acrostic, and provides a self-reflexive analysis of the content and the South Asian context from which the poems emerged. Each poem, I argue, grapples with various gendered structures of interpersonal and state violence, exacerbated by the COVID-19 pandemic and the lockdown that followed in its wake. In this article, I explore the ways in which the everydayness of violence can be documented through art and creative practices. My primary question is: What is the form of the poem doing for the content of the poem; that is, in what ways do certain poetic forms assist in the documentation of personal experiences of violence during a pandemic? This article explores the political possibilities offered by the method of writing form poetry as a documentation of violence, as well as providing a ‘witness’ to it. Thinking more about the role of producing art vis-à-vis my academic research, I further ask: How can we expand the scope of the feminist research methods we use, and what role might form poetry play in this? I situate this article at the intersection of South Asian Studies, Women’s and Gender Studies, and Literary and Cultural Studies, particularly focusing on the South Asian anglophone poetics of the written word in a post-pandemic time.
Advancing the Management of Long COVID by Integrating into Health Informatics Domain: Current and Future Perspectives
Authors: Ambalavanan Radha , Snead R Sterling , Marczika Julia , Kozinsky Karina , Aman Edris
Published: September 2023

The ongoing COVID-19 pandemic has profoundly affected millions of lives globally, with some individuals experiencing persistent symptoms even after recovering. Understanding and managing the long-term sequelae of COVID-19 is crucial for research, prevention, and control. To effectively monitor the health of those affected, maintaining up-to-date health records is essential, and digital health informatics apps for surveillance play a pivotal role. In this review, we overview the existing literature on identifying and characterizing long COVID manifestations through hierarchical classification based on Human Phenotype Ontology (HPO). We outline the aspects of the National COVID Cohort Collaborative (N3C) and Researching COVID to Enhance Recovery (RECOVER) initiative in artificial intelligence (AI) to identify long COVID. Through knowledge exploration, we present a concept map of clinical pathways for long COVID, which offers insights into the data required and explores innovative frameworks for health informatics apps for tackling the long-term effects of COVID-19. This study achieves two main objectives by comprehensively reviewing long COVID identification and characterization techniques, making it the first paper to explore incorporating long COVID as a variable risk factor within a digital health informatics application. By achieving these objectives, it provides valuable insights on long COVID’s challenges and impact on public health.
Bridging Inequity Gaps in Healthcare Systems While Educating Future Healthcare Professionals—The Social Health Bridge-Building Programme
Authors: Valentin Gitte , Nielsen Claus Vinther , Nielsen Anne-Sofie Meldgaard , Tonnesen Merete , Bliksted Kristina Louise , Jensen Katrine Tranberg , Ingerslev Karen , Maribo Thomas , Oestergaard Lisa Gregersen
Published: September 2023

Social inequity in healthcare persists even in countries with universal healthcare. The Social Health Bridge-Building Programme aims to reduce healthcare inequities. This paper provides a detailed description of the programme. The Template for Intervention Description and Replication (TIDieR) was used to structure the description. The programme theory was outlined using elements from the British Medical Research Council’s framework, including identifying barriers to healthcare, synthesising evidence, describing the theoretical framework, creating a logic model, and engaging stakeholders. In the Social Health Bridge-Building Programme, student volunteers accompany individuals to healthcare appointments and provide social support before, during, and after the visit. The programme is rooted in a recovery-oriented approach, emphasising personal resources and hope. The programme finds support in constructs within the health literacy framework. Student volunteers serve as health literacy mediators, supporting individuals in navigating the healthcare system while gaining knowledge and skills. This equips students for their forthcoming roles as healthcare professionals, and potentially empowers them to develop and implement egalitarian initiatives within the healthcare system, including initiatives that promote organisational health literacy responsiveness. The Social Health Bridge-Building Programme is a promising initiative that aims to improve equity in healthcare by addressing individual, social, and systemic barriers to healthcare. The programme’s description will guide forthcoming evaluations of its impact.
Geovisualization of Temporal Change in Urban Racial Geography: A Step towards Explaining Persistent Segregation
Authors: Stepinski Tomasz , Dmowska Anna
Published: September 2023

To better understand the persistence of residential racial segregation in U.S. cities, it is essential to develop testable, spatially explicit models of racial dynamics. However, the original census data are not formatted in a way that facilitates the testing of such models. In this study, we propose a novel geoprocessing pipeline that transforms census data into image-like geovisualization of urban segregation. The process consists of converting original data to a grid format, transforming subpopulation shares in each grid cell into a CMYK color, and multiyear clustering of CMYK values followed by classification and smoothing of images. When applied to data from multiple censuses, our methodology generates a time series of snapshots that depict the spatio-temporal dynamics of racial change. As a demonstration, we have utilized our methodology to generate racial images for a period spanning from 1990 to 2020 in seven major cities. Detailed results are shown for Chicago and Atlanta. By visually examining these time series, we have identified patterns that challenge the conventional Schelling model of racial change. Instead, our findings point towards a model that incorporates the interplay of preferential growth and diffusion as significant factors that shape the complex dynamics of racial composition in these urban areas.
Investigating the Spatiotemporal Relationship between the Built Environment and COVID-19 Transmission
Authors: Huang Hao , Shi Haochen , Zordan Mirna , Lo Siu Ming , Tsou Jin Yeu
Published: September 2023

Earlier studies have examined various factors that may contribute to the contagion rate of COVID-19, such as urban climatic and socioeconomic characteristics. However, there is a lack of studies at the township level detailing the spatiotemporal settings of built environment attributes, especially in the context of lockdown as a response to the global Omicron outbreak. In this study, we extended the existing literature by relating the initial-stage Omicron pandemic conditions with more comprehensive measures of the built environment, including density, diversity, design, distance to transit, and destination accessibility. The variations from the confirmed clusters of COVID-19 and asymptomatic infected cases before, during, and after the lockdown throughout the Omicron outbreak were identified geographically using GIS methods in 218 township-level divisions across Shanghai during the lockdown period. We also compared the regression results of the ordinary least-squares regression, geographically weighted regression, and geographically and temporally weighted regression. Our results show that (1) among all the built environment variables, metro line length, walking accessibility, hotel and inn density, and population exhibited positive significance in influencing pandemic prevalence; (2) spatial and temporal variations were evident in the association between accessibility, mobility, density-related built environment variables, and COVID-19 transmission across three phases: pre-lockdown, during lockdown, and post-lockdown. This study highlights the importance of targeted public health interventions in densely populated areas with high demand for public transit. It emphasizes the significance of transportation network layout and walking accessibility in controlling the spread of infectious diseases in specific urban contexts. By considering these factors, policymakers and stakeholders can foster urban resilience and effectively mitigate the impact of outbreaks, aligning with the objectives of the 2030 UN Sustainable Development Goals.
Assessment of Urban Resilience and Detection of Impact Factors Based on Spatial Autocorrelation Analysis and GeoDetector Model: A Case of Hunan Province
Authors: Chen Jianhong , Ma Hongcai , Yang Shan , Zhou Zhiyong , Huang Jianhui , Chen Licheng
Published: September 2023

The rapid development of urbanization has led to increasing uncertainties related to urban safety risks, which has brought certain challenges to the sustainable development of cities. The concept of urban resilience has found a new way to improve the ability of a city to absorb and resolve risks. However, the existing literature on the evaluation of urban resilience is mostly developed from a static perspective, lacking a systematic and dynamic understanding of the level of urban resilience. Therefore, this paper takes Hunan Province as the research object, determines the resilience evaluation indicators, collects the data of each indicator by using the observation method and the literature method, then chooses the comprehensive index method and other methods to measure the urban resilience level of Hunan Province in the years of 2010–2021, and observes the dynamic changes in the resilience level. And, we use the GeoDetector model to detect the dominant factors affecting the urban resilience level and the interaction between these factors. The results of this study show that: (1) The level of urban resilience in Hunan Province shows a steady upward trend from 2010 to 2021, but cities with low resilience levels hold a dominant position. Among all subsystems, the level of urban economic resilience is the highest. (2) From 2010 to 2021, the level of urban resilience in Hunan Province indicates a stepwise spatial structure in the spatial pattern, gradually decreasing from east to west. (3) The urban resilience of Hunan Province from 2010 to 2021 has a significant spatial agglomeration effect, mainly manifested as “L-H type” agglomeration and “L-L type” agglomeration. (4) The spatio-temporal differentiation of urban resilience is mainly caused by economic and social factors, while ecological, institutional, and infrastructure factors have a relatively small influence on the level of urban resilience. The interaction of impact factors will have a more significant influence on urban resilience. The research results of this article are of great significance for urban resilience construction in Hunan Province and even the whole country.
Anti-Inflammatory Effects of Tegoprazan in Lipopolysaccharide-Stimulated Bone-Marrow-Derived Macrophages
Authors: Han Gong-Ho , Kim Seong-Jun , Ko Wan-Kyu , Hong Je-Beom , Sheen Seung-Hun , Cho Min-Jai , Sohn Seil
Published: September 2023

The purpose of this study was to investigate the anti-inflammatory effect of tegoprazan (TEGO) in lipopolysaccharide (LPS)-stimulated bone-marrow-derived macrophages (BMMs). To this end, compared to methylprednisolone (MP; positive control), we evaluated whether TEGO effectively differentiates LPS-stimulated BMMs into M2-phenotype macrophages. Moreover, the expression of pro- and anti-inflammatory cytokines genes influenced by TEGO was measured using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. TEGO was found to reduce nitric oxide (NO) production in BMMs significantly. In addition, TEGO significantly decreased and increased the gene expression levels of pro-inflammatory and anti-inflammatory cytokines, respectively. In addition, we evaluated the phosphorylated values of the extracellular signal-regulatory kinase (ERK) and p38 in the mitogen-activated protein (MAP) kinase signaling pathway through Western blotting. TEGO significantly reduced the phosphorylated values of the ERK and p38. In other words, TEGO suppressed the various pro-inflammatory responses in LPS-induced BMMs. These results show that TEGO has the potential to be used as an anti-inflammatory agent.
Synthesis and Biological Activity of a New Indenoisoquinoline Copper Derivative as a Topoisomerase I Inhibitor
Authors: Molinaro Caroline , Wambang Nathalie , Pellegrini Sylvain , Henry Natacha , Lensink Marc F. , Germain Emmanuelle , Bousquet Till , de Ruyck Jérôme , Cailliau Katia , Pélinski Lydie , Martoriati Alain
Published: September 2023

Topoisomerases are interesting targets in cancer chemotherapy. Here, we describe the design and synthesis of a novel copper(II) indenoisoquinoline complex, WN198. The new organometallic compound exhibits a cytotoxic effect on five adenocarcinoma cell lines (MCF-7, MDA-MB-231, HeLa, HT-29, and DU-145) with the lowest IC50 (0.37 ± 0.04 μM) for the triple-negative MDA-MB-231 breast cancer cell line. Below 5 µM, WN198 was ineffective on non-tumorigenic epithelial breast MCF-10A cells and Xenopus oocyte G2/M transition or embryonic development. Moreover, cancer cell lines showed autophagy markers including Beclin-1 accumulation and LC3-II formation. The DNA interaction of this new compound was evaluated and the dose-dependent topoisomerase I activity starting at 1 μM was confirmed using in vitro tests and has intercalation properties into DNA shown by melting curves and fluorescence measurements. Molecular modeling showed that the main interaction occurs with the aromatic ring but copper stabilizes the molecule before binding and so can putatively increase the potency as well. In this way, copper-derived indenoisoquinoline topoisomerase I inhibitor WN198 is a promising antitumorigenic agent for the development of future DNA-damaging treatments.
Exploring the Drug-Loading and Release Ability of FucoPol Hydrogel Membranes
Authors: Araújo Diana , Martins Matilde , Freitas Filomena
Published: September 2023

The polysaccharide FucoPol has recently been shown to yield hydrogel membranes (HMs) characterized by good mechanical properties, biocompatibility, and anti-inflammatory activity that render them promising biomaterials for use in the biomedical field. Subsequently to such findings, envisaging their development into novel delivery systems for topical applications, in this study, FucoPol HMs prepared by crosslinking the biopolymer with iron cations were loaded with caffeine or diclofenac sodium as model drugs. Two loading methods, namely diffusion and mixing, were applied to evaluate the FucoPol’s HM drug-loading capacity and entrapment efficiency. The diffusion method led to a higher caffeine loading (101.9 ± 19.1 mg/g) in the HM1_DCAF membranes, while the mixing method resulted in a higher diclofenac sodium loading (82.3 ± 5.1 mg/g) in the HM1_DDS membranes. The HM1_DCAF membranes were characterized by increased mechanical and rheological parameters, such as their hardness (130.0 ± 5.3 kPa) and storage modulus (1014.9 ± 109.7 Pa), compared to the HM1_DDS membranes that exhibited lower values (7.3 ± 1.2 kPa and 19.8 ± 3.8 Pa, respectively), probably due to leaching occurring during the drug-loading process. The release profiles revealed a fast release of both APIs from the membranes loaded by diffusion, while a prolonged and sustained release was obtained from the membranes loaded by mixing. Moreover, for all API-loaded membranes, the release mechanism followed Fickian diffusion, with the release rate being essentially governed by the diffusion process. These findings, together with their previously shown biological properties, support the suitability of the developed FucoPol HMs to be used as platforms for the topical delivery of drugs.
In Vitro Models of Tissue and Organ Regeneration
Authors: Baer Patrick C. , Schubert Ralf
Published: September 2023

The recovery of cells after tissue and organ injury is a complex process [...]
Human Papillomavirus 16 DNA Methylation Patterns and Investigation of Integration Status in Head and Neck Cancer Cases
Authors: Zygouras Ioannis , Leventakou Danai , Pouliakis Abraham , Panagiotou Styliana , Tsakogiannis Dimitris , Konstantopoulos Georgios , Logotheti Eirini , Samaras Menelaos , Kyriakopoulou Zaharoula , Beloukas Apostolos , Pateras Ioannis S. , Delides Alexandros , Psyrri Amanda , Panayiotides Ioannis G. , Yiangou Minas , Kottaridi Christine
Published: September 2023

Persistent high-risk human papillomavirus (HPV) infection is a pivotal factor in the progression of cervical cancer. In recent years, an increasing interest has emerged in comprehending the influence of HPV on head and neck squamous cell carcinoma (HNSCC). Notably, it is well established that HPV-associated HNSCC show cases with distinct molecular and clinical attributes compared to HPV-negative cases. The present study delves into the epigenetic landscape of HPV16, specifically its L1 gene and untranslated region (UTR), through pyrosequencing, while the HPV16 DNA physical status was evaluated using E2/E6 ratio analysis in HPV16-positive HNSCC FFPE biopsies. Our findings reveal substantial methylation across six sites within the HPV16 L1 gene and seven sites in the UTR. Specifically, methylation percentages of two L1 CpG sites (7136, 7145) exhibit significant associations with tumor histological grade (p < 0.01), while proving concurrent methylation across multiple sites. The HPV16 DNA physical status was not correlated with the methylation of viral genome or tumor characteristics. This is the first study that examines epigenetic modifications and the HPV16 DNA physical status in Greek HNSCC patients. Our findings suggest an orchestrated epigenetic modulation among specific sites, impacting viral gene expression and intricate virus–host interactions.
The Effect of Silver Nanoparticles on the Digestive System, Gonad Morphology, and Physiology of Butterfly Splitfin (Ameca splendens)
Authors: Kamaszewski Maciej , Kawalski Kacper , Wiechetek Wiktoria , Szudrowicz Hubert , Martynow Jakub , Adamek-Urbańska Dobrochna , Łosiewicz Bogumił , Szczepański Adrian , Bujarski Patryk , Frankowska-Łukawska Justyna , Chwaściński Aleksander , Aksakal Ercüment
Published: September 2023

The aim of this study was to determine the effects of silver nanoparticles (AgNPs) on the morphology and enzymatic activity of butterfly splitfin (Ameca splendens). Individuals of both sexes, aged about five months, were exposed to AgNPs at concentrations of 0 (control group), 0.01, 0.1, and 1.0 mg/dm3 for 42 days. On the last day of the experiment, the fish were euthanized, subjected to standard histological processing (anterior intestine, liver, and gonads), and analysed for digestive enzyme activity in the anterior intestine and oxidative stress markers in the liver. Fish in the AgNP 0.01 and 0.1 groups had the lowest anterior intestinal fold and enterocyte height. However, there were no statistically significant changes in the digestive enzyme activity in the anterior intestine. Analysis of enzymatic activity in the liver showed an increase in superoxide dismutase activity in fish in the AgNP 0.1 group. Histological analyses showed that AgNPs inhibited meiotic divisions at prophase I in a non-linear manner in ovaries and testes. In the AgNP 0.1 and 1.0 groups, the area occupied by spermatocytes was lower compared to the other groups. These results indicate that exposure to AgNPs may lead to disturbances in morphology and enzymatic activity in the liver and intestine and may lead to disruption of reproduction in populations.
Mediterranean Diet and Oxidative Stress: A Relationship with Pain Perception in Endometriosis
Authors: Cirillo Michela , Argento Flavia Rita , Becatti Matteo , Fiorillo Claudia , Coccia Maria Elisabetta , Fatini Cinzia
Published: September 2023

Background: Endometriosis is a chronic and inflammatory disease associated with pelvic pain. Dietary changes may be therapeutic for chronic inflammatory processes, reducing visceral input. The aim was to evaluate the role of dietary changes according to the Mediterranean Diet (MD) on pain perception in endometriosis and their relationship with oxidative stress. Methods: in this prospective study, we included 35 endometriosis women. At baseline (T0) and after 3 (T1) and 6 (T2) months from the start of the diet, we investigated pain intensity with VAS (Visual Analogue Scale, from 0 to 10), vitamin profile, and oxidative stress. Results: we found a significant increase in the diet score (p < 0.001). At T1, patients reduced pain in terms of dyspareunia (p = 0.04), non-menstrual pelvic pain (p = 0.06), dysuria (p = 0.04), and dyschezia (p < 0.001). Dyspareunia (p = 0.002) and dyschezia (p < 0.001) were further significantly reduced also at T2. We observed a significant positive correlation between lipid peroxidation and VAS non-menstrual pelvic pain and dysuria and a significant negative correlation between Oxygen radical absorbance capacity and VAS non-menstrual pain and dyschezia. Conclusions: our findings show a clear tendency toward a relationship between pain relief in endometriosis and MD. This appears promising to treat endometriosis-related symptoms and could be considered a new effective strategy for chronic pain management in the long term.
Overview and Recent Progress on the Biosynthesis and Regulation of Flavonoids in Ginkgo biloba L.
Authors: Guo Jing , Wang Yeqiao , Li Jiaqi , Zhang Jingjing , Wu Yaqiong , Wang Guibin
Published: September 2023

Flavonoids and their derivatives play important roles in plants, such as exerting protective activity against biotic and abiotic stresses, functioning in visual signaling to attract pollinators, and regulating phytohormone activity. They are also important secondary metabolites that are beneficial to humans. Ginkgo biloba L. is a well-known relict plant considered to be a “living fossil”. Flavonoids present in ginkgo leaves have antioxidant and anti-aging capacities and show good therapeutic effects on a variety of neurological diseases. To date, studies on flavonoids have mainly focused on their extraction, pharmacological effects, and component analysis and on the expression levels of the key genes involved. However, a systematic review summarizing the biosynthesis and regulatory mechanisms of ginkgo flavonoids is still lacking. Thus, this review was conducted to comprehensively introduce the biological characteristics, value, and utilization status of ginkgo; summarize the effects, biosynthetic pathways, and transcriptional regulation of flavonoids; and finally, discuss the factors (ecological factors, hormones, etc.) that regulate the biosynthesis of flavonoids in ginkgo. This review will provide a reference basis for future research on the biosynthesis and efficient utilization of flavonoids in ginkgo.
Salivary Cystatin D Interactome in Patients with Systemic Mastocytosis: An Exploratory Study
Authors: Serrao Simone , Contini Cristina , Guadalupi Giulia , Olianas Alessandra , Lai Greca , Messana Irene , Castagnola Massimo , Costanzo Giulia , Firinu Davide , Del Giacco Stefano , Manconi Barbara , Cabras Tiziana
Published: September 2023

Mastocytosis, a rare blood disorder characterized by the proliferation of clonal abnormal mast cells, has a variegated clinical spectrum and diagnosis is often difficult and delayed. Recently we proposed the cathepsin inhibitor cystatin D-R26 as a salivary candidate biomarker of systemic mastocytosis (SM). Its C26 variant is able to form multiprotein complexes (mPCs) and since protein–protein interactions (PPIs) are crucial for studying disease pathogenesis, potential markers, and therapeutic targets, we aimed to define the protein composition of the salivary cystatin D-C26 interactome associated with SM. An exploratory affinity purification-mass spectrometry method was applied on pooled salivary samples from SM patients, SM patient subgroups with and without cutaneous symptoms (SM+C and SM−C), and healthy controls (Ctrls). Interactors specifically detected in Ctrls were found to be implicated in networks associated with cell and tissue homeostasis, innate system, endopeptidase regulation, and antimicrobial protection. Interactors distinctive of SM−C patients participate to PPI networks related to glucose metabolism, protein S-nitrosylation, antibacterial humoral response, and neutrophil degranulation, while interactors specific to SM+C were mainly associated with epithelial and keratinocyte differentiation, cytoskeleton rearrangement, and immune response pathways. Proteins sensitive to redox changes, as well as proteins with immunomodulatory properties and activating mast cells, were identified in patients; many of them were involved directly in cytoskeleton rearrangement, a process crucial for mast cell activation. Although preliminary, these results demonstrate that PPI alterations of the cystatin D-C26 interactome are associated with SM and provide a basis for future investigations based on quantitative proteomic analysis and immune validation.
Regulation of Ferroptosis in Lung Adenocarcinoma
Authors: Wei Xiangyun , Li Xiaohe , Hu Shuming , Cheng Jinke , Cai Rong
Published: September 2023

Lung adenocarcinoma (LUAD) is the most common lung cancer, which accounts for about 35–40% of all lung cancer patients. Despite therapeutic advancements in recent years, the overall survival time of LUAD patients still remains poor, especially KRAS mutant LUAD. Therefore, it is necessary to further explore novel targets and drugs to improve the prognos is for LUAD. Ferroptosis, an iron-dependent regulated cell death (RCD) caused by lipid peroxidation, has attracted much attention recently as an alternative target for apoptosis in LUAD therapy. Ferroptosis has been found to be closely related to LUAD at every stage, including initiation, proliferation, and progression. In this review, we will provide a comprehensive overview of ferroptosis mechanisms, its regulation in LUAD, and the application of targeting ferroptosis for LUAD therapy.
Low Levels of Amyloid Precursor Protein (APP) Promote Neurogenesis and Decrease Gliogenesis in Human Neural Stem Cells
Authors: Coronel Raquel , López-Alonso Victoria , Gallego Marta I. , Liste Isabel
Published: September 2023

Amyloid precursor protein (APP) has been widely studied due to its association with Alzheimer’s disease (AD). However, the physiological functions of APP are still largely unexplored. APP is a transmembrane glycoprotein whose expression in humans is abundant in the central nervous system. Specifically, several studies have revealed the high expression of APP during brain development. Previous studies in our laboratory revealed that a transient increase in APP expression induces early cell cycle exit of human neural stem cells (hNSCs) and directs their differentiation towards glial cells (gliogenesis) while decreasing their differentiation towards neurons (neurogenesis). In the present study, we have evaluated the intrinsic cellular effects of APP down-expression (using siRNA) on cell death, cell proliferation, and cell fate specification of hNSCs. Our data indicate that APP silencing causes cellular effects opposite to those obtained in previous APP overexpression assays, inducing cell proliferation in hNS1 cells (a model line of hNSCs) and favoring neurogenesis instead of gliogenesis in these cells. In addition, we have analyzed the gene and protein expression levels of β-Catenin as a possible molecule involved in these cellular effects. These data could help to understand the biological role of APP, which is necessary to deepen the knowledge of AD.
Monitoring Key Pair Usage through Distributed Ledgers and One-Time Signatures
Authors: Mayr Lucas , Palma Lucas , Zambonin Gustavo , Silvano Wellington , Custódio Ricardo
Published: September 2023

Private key management is a complex obstacle arising from the traditional public key infrastructure model. However, before any related security breach can be addressed, it must first be reliably detected. Certificate Transparency (CT) is an example of a certificate issuance monitoring strategy, developed to detect the possible malfeasance of certification authorities (CAs). To the best of our knowledge, CT and other detection mechanisms do not cover digitally signed documents made by an end user, which are also susceptible to CA misbehavior. We modify the CT framework to handle signed documents via logging certificates in the blockchain to enable the secure and user-friendly monitoring of one-time signatures, backdating protection, and effective CA misbehavior detection. Moreover, to demonstrate the feasibility of our proposal, we present distinct deployment scenarios and analyze the storage, performance, and monetary costs.
Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in Solenopsis invicta Buren (Hymenoptera: Formicidae)
Authors: Miles Godfrey P. , Liu Xiaofen F. , Amiri Esmaeil , Grodowitz Michael J. , Allen Margaret L. , Chen Jian
Published: September 2023

Deformed wing virus (DWV), a major honey bee pathogen, is a generalist insect virus detected in diverse insect phyla, including numerous ant genera. Its clinical symptoms have only been reported in honey bees, bumble bees, and wasps. DWV is a quasispecies virus with three main variants, which, in association with the ectoparasitic mite, Varroa destructor, causes wing deformity, shortened abdomens, neurological impairments, and colony mortality in honey bees. The red imported fire ant, Solenopsis invicta Buren, is one of the most-invasive and detrimental pests in the world. In this study, we report the co-occurrence of DWV-like symptoms in S. invicta and DWV for the first time and provide molecular evidence of viral replication in S. invicta. Some alates in 17 of 23 (74%) lab colonies and 9 of 14 (64%) field colonies displayed deformed wings (DWs), ranging from a single crumpled wing tip to twisted, shriveled wings. Numerous symptomatic alates also exhibited altered locomotion ranging from an altered gait to the inability to walk. Deformed wings may prevent S. invicta alates from reproducing since mating only occurs during a nuptial flight. The results from conventional RT-PCR and Sanger sequencing confirmed the presence of DWV-A, and viral replication of DWV was confirmed using a modified strand-specific RT-PCR. Our results suggest that S. invicta can potentially be an alternative and reservoir host for DWV. However, further research is needed to determine whether DWV is the infectious agent that causes the DW syndrome in S. invicta.
Deviation of Latitude Cut: A Simple Sign to Differentiate Total Hip Arthroplasty from Hemiarthroplasty in Radiography
Authors: Park Sunghoon , Yun Jae Sung , Bang Dong-Ho , Jung Yongjun , Kwack Kyu-Sung , Kim Jung-Taek
Published: September 2023

This study aims to propose latitude cut deviation for differentiating hip arthroplasty types and evaluate its diagnostic utility in distinguishing total hip arthroplasty (THA) from hemiarthroplasty using radiography. After assessing various cup designs from top manufacturers for THA and hemiarthroplasty, we conducted a retrospective study on 40 patients (20 THA and 20 hemiarthroplasty). Three readers independently evaluated the radiographs, assessing acetabular sparing, cup–bone interface texture, and latitude cut deviation. Diagnostic performance and inter-observer agreement were compared using receiver operating characteristic curves and the Fleiss kappa coefficient. Latitude cut deviation measured on implant designs ranged from 19% to 42% in hemiarthroplasty and from −12% to 9% in THA. The sensitivity, specificity, and accuracy used to distinguish THA from hemiarthroplasty were 60–85%, 55–95%, and 62.5–77.5% for acetabular sparing; 100%, 50–80%, and 75–90% for cup–bone interface texture; and 100%, 90–100%, and 95–100% for latitude cut deviation. Inter-observer agreement for acetabular sparing, cup–bone interface texture, and latitude cut deviation ranged from moderate to excellent (κ = 0.499, 0.772, and 0.900, respectively). The latitude cut deviation exhibited excellent diagnostic performance and inter-reader agreement in distinguishing hemiarthroplasty from THA on radiographs, offering a concise way to identify hip arthroplasty type.
Treatment of Fracture-Related Infection after Pelvic Fracture
Authors: Freigang Viola , Walter Nike , Rupp Markus , Riedl Moritz , Alt Volker , Baumann Florian
Published: September 2023

Background: The management of pelvic fractures is a significant challenge. Surgical site infection can result in the need for revision surgery, cause functional impairment, and lead to a prolonged length of stay and increased treatment costs. Although reports on fracture-related infection (FRI) after pelvic fracture fixation are sparsely reported in the literature, it is a serious complication. This study analysed patients with FRIs after pelvic fracture regarding patient characteristics, treatment strategies, and an evaluation of risk factors for FRI. Methods: In this retrospective single-centre study, FRI was diagnosed based on clinical symptoms of infection and a positive culture of a bacterial infection. Depending on the severity and acuteness of the infection, osseous stabilization was restored either via implant retention (stable implant, no osteolysis), exchange (loose implant or bony defect), or external fixation (recurrence of infection after prior implant retaining revision). Healing of infection was defined as no sign of recurring infection upon clinical, radiological, and laboratory examination in the last follow-up visit. Results: The FRI rate in our patient population was 7.5% (24/316). In 8/24 patients, the FRI occurred within the first three weeks after initial surgery (early) and 16/24 presented with a late onset of symptoms of FRI. A strategy of debridement, antibiotics, and implant retention (DAIR) was successful in 9/24 patients with FRI after pelvic fracture. A total of 10 patients required an exchange of osteo-synthetic implants, whereof three were exchanged to an external fixator. In five patients, we removed the implant because the fracture had already consolidated at the time of revision for infection. A total of 17/24 patients had a poly-microbial infection after a pelvic fracture and 3/24 patients died from post-traumatic multi-organ failure within the first 6 months after trauma. There were no cases of persistent infection within the remaining 21 patients. Conclusions: Although poly-microbial infection is common in FRI after pelvic fracture, the recurrence rate of infection is relatively low. A complex pelvic trauma with significant soft tissue injury is a risk factor for recurrent infection and multiple revisions. A strategy of DAIR can be successful in patients with a stable implant. In cases with recurrent infection or an unstable fracture site, the exchange of implants should be considered.