bluuebunny/crossref_metadata_2025 huggingface dataset:
Eigenvector index for two female fertility traits based in random regression coefficients matrix in Holstein cows
Authors: Ghiasi Heydar , Carabaño Maria J.
Published: March 2018

A total of 71,518 days open (DO) and number of services per conception (NSC) records of 28,523 Iranian Holstein cows were analysed by random regression model. Akaike’s information criterion and likelihood ratio test suggested that a model with quadratic Legendre polynomials for additive genetic and permanent environmental was best. Heritability in different parities ranged from 0.103 to 0.045 in first parity and 0.054 to 0.030 in sixth parity for DO and NSC, respectively. Estimated genetic correlations between parities decreased continuously with increasing distance between parities for both DO and NSC. The first eigenfunction explained 89.99% and 97.22 % of the total genetic variance of DO and NSC, while the second eigenfunction accounted 6.24% and 3.18%, respectively. Different selection indices were constructed for DO and NSC. Genetic response to improve overall fertility was greater when the index included the first eigenvector than the response obtained from indices excluding it. Similar genetic gains were obtained from the first eigenvector, which had a nearly flat associated eigenfunction along lactations and from selection by the intercept of the random regression. The first eigenvector indices were responsible of changes in the level of DO and NSC in a similar manner for all parities, without altering the shape of the response curve across parities. The second and third eigenvector indices modified the shape of this curve but the improvement in genetic gains by including them in the selection index were small (DO) or negligible (NSC) due to the small amount of variability associated with these components.
Patient participation in the development of a customized m-Health intervention to improve medication adherence in poorly adherent individuals with bipolar disorder (BD) and hypertension (HTN)
Authors: Blixen Carol , Sajatovic Martha , Moore David J. , Depp Colin , Cushman Clint , Cage Jamie , Barboza Marina , Eskew Logan , Klein Peter , Levin Jennifer B.
Published: March 2018

Objective: Individuals living with bipolar disorder (BD) have poorer management of chronic medical conditions such as hypertension (HTN), and worse treatment adherence than the general population. The study objective was to obtain information from patients with both BD and HTN that would inform the development of an m-Health intervention to improve medication adherence for poorly adherent individuals living with both these chronic illnesses.Methods: Focus group methodology was used to collect information from 13 participants on perceived barriers and facilitators to BD and HTN medication adherence, as well as feedback on the demonstration and use of a bidirectional text messaging system for medication reminders. Focus groups were audiotaped, transcribed verbatim, and analyzed using content analysis with an emphasis on dominant themes.Results: Forgetfulness was the most frequently mentioned barrier to taking antihypertensive medications, and decisions about taking them were often influenced by BD mood fluctuations and the burden of having to take “too many pills” for both chronic illnesses. Participants’ feedback about the use of a text-messaging system to help with medication adherence for BD and HTN was very positive, and their suggestions for modification were incorporated into a more customized system for testing in a Phase 2 trial.Conclusions: Our findings indicate that patient engagement in the development of an m-Health intervention has the potential to improve adherence with both BD and HTN medications in individuals with known sub-optimal adherence. Patient engagement in health care is essential if we are to optimize patient outcomes.
Estrategias y razones del impacto de WikiLeaks en la opinión pública mundial
Authors: Quian Alberto , Elías Carlos
Published: March 2018

Esta investigación intenta explicar cuándo, cómo y por qué emerge WikiLeaks como fenómeno global sistematizando sus estrategias mediáticas en tres claras etapas. Usamos herramientas de analítica web para recoger datos de cuatro niveles de popularidad digital: búsquedas en Google, acceso al sitio web de WikiLeaks, presencia y seguidores en Twitter. Se analizan más de 5 años (desde su nacimiento en 2006 hasta la filtración de Stratfor en 2012). Nuestros resultados indican que, en la era de la sociedad red, la popularidad de un fenómeno digital y antisistema como WikiLeaks (y el impacto de sus mensajes) depende, paradójicamente, de su difusión en la prensa tradicional de papel (quality press)que aún conserva su influencia y poder legitimador.
Associations of Herbicides on the Simultaneous Control of Horseweed and Italian Ryegrass
Authors: Marchioretto Lucas de Ross , Basso Ferrazzi Bruno Vicente , Dal Magro Taísa , Damiani Conte Elaine
Published: March 2018

Weed competition negatively affects grain yield on wheat and soybean crops. Due to better practicality and timesaving it is usual the association of broadleaf herbicides with graminicides to control monocot and dicot weeds. However, little is known about the deleterious effects of broadleaf herbicides on graminicides. Thus, the objective of this experiment was to evaluate the effects of simple, double and triple association of broadleaf herbicides with graminicides to control of Italian ryegrass and horseweed. The experiment was conducted into greenhouse on completely randomized experimental design with four replications. The treatments consisted of the graminicides clethodim and clodinafop; the broadleaf herbicides 2,4-D, metsulfuron and saflufenacil and non-selective glyphosate, sprayed isolated and in double and triple association to be compared with a control. The variables evaluated were control (%) at 21 and 28 days after treatments (DAT), and dry weight (g) at 28 DAT. The isolated graminicides and the broadleaf herbicides controlled both Italian ryegrass and horseweed respectively. At the double association of broadleaf with graminicides herbicides, 2,4-D antagonized the effect of clodinafop, and glyphosate mixed with clodinafop decreased the control of the graminicide on Italian ryegrass. At the triple herbicide association, when mixed with glyphosate, metsulfuron decreased the control effect of Italian ryegrass by clethodim. The association of broadleaf herbicides with graminicides is effective on the control of monocot and dicot weeds, but it depends on which herbicide used for the combination. When it is evident the antagonism between broadleaf herbicides and graminicides, the detrimental effects go on grass control.
Effect of Different Rates of Poultry Manure and Bio-Slurry on the Yield of Solanum aethiopicum Shum
Authors: Nanyanzi Mary , Kizito Elizabeth Balyejusa , Masanza Michael , Sseruwu Godfrey , Tenywa Moses Makoma
Published: March 2018

Poor soil fertility remains the major cause of low crop productivity on smallholder farms that are engaging in vegetable production in sub-Saharan Africa. Appropriate soil fertility regimes are therefore critical for improving crop productivity. Its yield has remained low mainly due to poor soil fertility. A field experiment in two different seasons was planted in a Completely Randomized Block Design using Solanum aethiopicum Shum (Nakati). The treatments were 3 sole fertilizer options applied at the following rates: poultry manure and bio-slurry manure at 5, 10, 15, 20, 25 and 30 t ha-1, NPK (25:5:5) at the recommend application rate for tomato which is a sister crop and a control without any fertilizer. Crop budgets were used to determine the economic optimum rates of both sole applications of manure and combinations of manure with NPK. The sole applications and showed significantly (p ≤ 0.05) increased the yield of S. aethiopicum compared with the control. The established biological optimum rates were at 24.19 t ha-1 and 21.51 t ha-1 for poultry manure and bio-slurry respectively. Using the crop budgets it was concluded that the established economic optimum rates were 20 t ha-1 and 10 t ha-1 for sole poultry manure and bio-slurry respectively. Recommendations for use of sole poultry manure and bio- slurry at the rate of 20 t ha-1 and 10 t ha-1 respectively were made.
Pre-Weaning Growth Performance of Piglets at Smallholder Farms in Gauteng Province
Authors: Matabane Matshidiso B. , Nephawe Khathutshelo A. , Thomas Ronald S. , Maqhashu Ayanda , Ramukhithi Fhulufhelo V. , Netshirovha Thivhilaheli R. , Ng’ambi Jones W. , Tsatsimpe Mammikele , Nedambale Tshimangadzo L.
Published: March 2018

The objective of the study was to determine pre-weaning performance of piglets born following artificial insemination (AI) at smallholder farms of Gauteng province. Data from 496 piglets originating from 73 multiparous crossbred sows were used in the study. Litter size, number of piglets born alive, number of piglets weaned, birth and weaning weights were recorded. Data was analysed using the Proc Univariate procedure of SAS. The average litter size was 11.8. The average birth weight and weaning weights were 1.9 and 6.2 kg, respectively. No significant differences were found between male and female piglets for all the growth performance characteristics. Piglets born during winter had a significantly higher (P < 0.05) birth and weaning weight as compared to autumn and summer months. Season had a significant effect on birth and weaning weight (P < 0.01). However, sex of piglets had no significant effect on all the characteristics recorded (P > 0.05). The interaction between sex and season was only confirmed on the total number of weaned piglets (P < 0.01). A highly significant positive correlation was found between litter size and number of piglets born alive (r = 0.86) and total number of piglets weaned (r = 0.50). A highly significant correlation was found between total number of piglets born alive and total number of piglets weaned (r = 0.55). In conclusion, season of birth had the greatest impact on birth and weaning weight, with the highest birth and weaning weights recorded during winter season. However, sex did not affect the pre-weaning performance of piglets.
Effect of Farmyard Manure, Lime and Inorganic Fertilizer Applications on Soil pH, Nutrients Uptake, Growth and Nodulation of Soybean in Acid Soils of Western Kenya
Authors: Otieno Hillary M. O. , Chemining’wa George N. , Zingore Shamie
Published: March 2018

Soybean is an important crop with nutrition, economic and soil fertility improvement benefits to farmers. However, its production in western Kenya is partly constrained by low soil pH and soil fertility levels. A greenhouse pot study was conducted to evaluate the effects of inorganic fertilizers, farmyard manure and lime application on soil pH, nutrient uptake, growth and nodulation of soybean grown in acidic soils from western Kenya. The experiment was set up in a randomized complete block design with eight treatments: control; NPK; manure; lime; manure+lime; NPK+manure; NPK+lime and manure+lime+NPK. Significant effects of inorganic fertilizers, manure and lime applications were observed on all the variables. Manure, lime and manure+lime treatments increased soil pH by 1.33, 2.19 and 2.28, respectively, above the control treatment. The shoot N was lower under control (1.71-1.81%), NPK (1.85-1.98%) and manure (2.00-2.11%) than under all other treatments. Treatments NPK+lime and manure+lime+NPK recorded higher uptake of P and K nutrients than all other treatments. The control and NPK treatments recorded shorter plants and low leaf area and above-ground biomass compared to other treatments. The NPK+lime and manure+lime+NPK treatments recorded higher plant height and aboveground biomass than other treatments. Lime+manure treatment recorded highest nodule numbers and dry weights per plant. Positive relationships were observed between pH and N, P and K nutrient uptake (R2 ranged between 0.30 and 0.77) and between biomass and N, P and K nutrient uptake (R2 ranged between 0.68 and 0.99). From this study, use of manure and lime could result in improved soil pH for better uptake of nutrients, nodule formation and productivity of soybean in Western Kenya.
Morphological, Physiological and Nutritional Effects of Irrigation Frequency on Macaúba Palm Seedlings
Authors: Hernández C. P. , Imbuzeiro Hewlley Acioli , Pimentel Leonardo Duarte , Hamakawa Paulo José , Both Arend-Jan
Published: March 2018

The commercial nurseries of macaúba palm (Acrocomia aculeata) have experienced excessive seedling mortality, which is possibly due to the lack of information about the proper growing practices. The goal of this study was to investigate the response of macaúba seedlings to water stress using different irrigation frequencies during the early seedling stage. The experiment was carried out in a macaúba palm nursery located in João Pinheiro, Minas Gerais, Brazil. The treatments started 30 days after sowing. At 90 days after sowing, the morphological (number of leaves, stem diameter, shoot length, root weight and the fresh and dry matter content of leaves, stem and roots), physiological (allocation of photosynthates) and chemical (nutrient content in the shoot) characteristics were evaluated. The results indicated that shoot height and stem diameter became smaller as the frequency of irrigation was reduced. However, the accumulation of fresh biomass in leaves and stems, and the leaf and root dry biomass became higher under the same condition. When water stress was induced in younger seedlings, the allocation of photosynthates occured more proportionally across all organs of the plants. The highest accumulation of K, S and Fe in the shoot were observed in plants that were exposed to water stress. N accumulation was higher in the aerial part of the seedlings as the irrigation frequency increased. However, the opposite behavior was observed for P, which accumulation was lowest in the control treatments.
Soybean Yield, Soil Porosity and Soil Penetration Resistance under Mechanical Scarification in No-Tillage System
Authors: Seidel Edleusa Pereira , Schneider Ana Paula Heck , Sustakowski Monica Carolina , Matté Lucas Murakami , Mottin Marcos Cesar , Silva João Henrique
Published: March 2018

Technological development has triggered a steady increase in Brazilian agricultural production, but also brought problems due to the excessive land use. The lack of care with proper management practices has led to soil physical degradation, mainly the formation of impermeable layers, which can lead to a reverse effect, a reduction in crop yield. It can be potentiated in silage production and with the lack of cover crops. To minimize the negative impacts of soil compaction, scarification is recommended, but its effectiveness has been questioned in no-tillage system. Thus, an experimental field was implemented in Brazil in 2015-2016 season, to evaluate the mechanical scarification on soybean production in succession to silage and grain corn intercropped with Brachiaria (Urochloa ruziziensis) as well as the physical properties of the soil. The experimental layout was a complete randomized block design with four replications. The plots were composed of second crop maize (autumn) intercropped with brachiaria, in two systems: silage and dry grains. The subplots were composed of three management system: no-tillage, reduced tillage cultivation with Terrus scarifier and Fox scarifier. Corn harvesting systems as well the scarifiers use did not affect soybean production and its yield components. The use of scarifiers reduced soil coverage, plant population, and soil penetration resistance. The data suggest that there was no persistence in the benefits presented by scarification. Soybean was able to break through the compacted layers, even above the critical level, corroborating with the hypothesis that the use of scarifiers does not bring benefits in no-tillage system.
Yield of the Okra Submitted to Nitrogen Rates and Wastewater in Northeast Brazilian Semiarid Region
Authors: Medeiros Aldair De Souza , Queiroz Manoel Moisés Ferreira de , Araújo Neto Renato A. de , Costa Patrícia Da Silva , Campos Amanda Costa , Ferraz Rener Luciano de Souza , Magalhães Ivomberg Dourado , Maia Júnior Sebastião De Oliveira , Melo Luan Danilo Ferreira de Andrade , Gonzaga Giordano Bruno Medeiros
Published: March 2018

Water is an indispensable resource for the maintenance of life; however, the available volume for consumption has decreased over a period of life, as a result of which, the availability of water that is inferior in quality has increased. In this context, we aimed to evaluate the growth and yield of okra (Santa Cruz cultivar) under different nitrogen rates and irrigation facilities using post-treated domestic wastewater through sand filter with intermittent flow in a Brazilian semiarid region. The experiment was performed in the Pombal region of the Paraíba state, Brazil using a randomized block design with six nitrogen Rates (N1 = 0, N2 = 40, N3 = 80, N4 = 120, N5 = 160, and N6 = 200 kg ha-1) and irrigation by using wastewater. The water was added to the treatment with 100% (160 kg ha-1) using nitrogen fertilization recommendation and irrigation water supply. The effects of treatments on the growth and production variables of okra plants were evaluated.
Tools for Rethinking Classroom Participation in Secondary Mathematics
Authors: Rubel Laurie H. , Stachelek Anders J.
Published: March 2018

In this article, we share our experiences in mathematics teacher education around professional development for teachers with a focus on student participation as an opportunity to learn. We describe a process through which teacher educators can support teachers in increasing and improving classroom participation opportunities for their students. We present Lesson Activity Bars and Difference in Participation Proportion, complementary tools that quantify and represent student participation in the mathematics classroom. We demonstrate the effectiveness of these tools in supporting teacher growth in the context of a professional growth project for teachers in urban secondary schools. In general, the teachers in this project increased the amounts of active participation they made available to their students. The cases of two teachers are analyzed in detail, using Clarke and Hollingsworth's (2002) Interconnected Model of Professional Growth, to add depth and nuance to our understanding of processes of teacher growth around increasing student participation opportunities in the mathematics classroom.
Using Online Collaboration to Improve Prospective Teachers' Analysis of Teaching
Authors: Spitzer Sandy , Phelps-Gregory Christine
Published: March 2018

For prospective teachers (PTs) to engage in lifelong systematic learning, they must be prepared to analyze teaching on the basis of its effects on student learning. We present the results of an intervention study aimed at developing PTs' ability to analyze a classroom video sample. The intervention used an online discussion board activity structured along three research-based dimensions, which allowed PTs to build their analysis skills outside of class time. Evidence for the effectiveness of this intervention includes findings that PTs engaged deeply with their peers' ideas, with many changing their mind about the lesson's success, and that PTs' final reflections showed increased attention to the mathematics of the learning goal. However, after the intervention, many PTs continued to take nonmathematical evidence as indicators of student learning. Implications illuminate key design features of interventions as well as the affordances and challenges of using online interactions for improving PTs' lesson analysis skills.
When Mathematics Teacher Educators Come Under Attack
Authors: Gutiérrez Rochelle
Published: March 2018

We are in an interesting historical moment in mathematics teacher education. On the one and, there is greater realization within our field of the connections between systems of power and mathematics (O'Neil, 2016). We are starting to acknowledge how mathematics education can be viewed as dehumanizing for both students and teachers as well as what might constitute rehumanizing practices (Gutiérrez, in press). Our professional organizations are calling for teachers to move beyond simplistic notions of equity to understand these power dimensions and challenge the system on behalf of (and in community with) Black,1 Indigenous,2 and Latinx3 students in particular
Case of late hematoma after breast augmentation
Authors: Kim Leslie , Castel Nikki , Parsa Fereydoun Don
Published: March 2018

We present a case report of a patient who experienced a late, spontaneous breast hematoma 26 years after primary breast augmentation. Late hematomas are a rare complication of breast augmentation with uncertain etiology. In this case, there was no trauma, calcifications, or implant rupture. We believe the patient’s hematoma was secondary to erosion of a capsular vessel due to capsular contracture.
Ozone Therapy: mechanisms of action, recent literature and newly discovered biochemical pathways [abstract]
Authors: Re Lamberto
Published: March 2018

Ozone therapy is widely used in many countries since many years. Recently, the increasing widespread of this complementary therapy has been accomplished by an increased number of basic and clinic papers published on international journals. This lecture will deal on the first approach by a pharmacological point of view in the aim to characterize the mechanisms activated at sub-cellular level by ozone when used for medical and beauty application at low graded doses.

The first theory was based upon the fact that the exposure to low, non-toxic, ozone concentrations could increase the efficacy of the endogenous antioxidant system by increasing the production or the activity of some enzymes exerting a key role in the mitochondrial respiratory chain.

Many of the basic mechanisms of the ozone action are now well outlined. In addition, the modulation of interleukins productions and of some biochemical pathways related to inflammation and pain, indicates the rationale of its use in many pathological conditions related to pain, inflammation and age disorders.

We also discuss on the mode of action of ozone that, with an hormetic mechanism, appear to be more similar to xenobiotic stress model than a pure pharmacological action.

Indeed, our recent work in vivo on patients treated with major autohemo therapy (MAH) demonstrated the increase of Nrf2 level (P
Ozone Therapy as a treatment modality for temporo-mandibular joint dysfunction [abstract]
Authors: Daif Emad T. , Basha Hamdy Y.
Published: March 2018

Temporo-mandibular (TMJ) disorders are of multi-factorial etiology. Numerous attempts have been made to identify the causes however, a great controversy still exists. Emotional stress, malocclusion and bruxism of the teeth are thought to be important factors in TMJ disorders. The limitations of mouth opening, deviation of the mandible on opening, joint noises and pain are among the common signs and symptoms of TMJ disorders. Concerning the treatment, different surgical and non- surgical techniques have been tied by several clinicians. In the recent years, ozone therapy is tried as a new treatment modality for many diseases particularly joint affection. So this study was carried out to assess the clinical outcome of ozone therapy in TMJ disorders.

Twenty five patients (16 Females, 9 Males) complaining of TMJ disorders were selected for this study. Every affected joint received 2 ml of ozone gas in concentration of 10 ?g/ml by direct injection of the gas into the joint cavity. The injection was repeated two times per week for tree weeks. The clinical signs and symptoms before and after treatment were assessed according to Helkimo's clinical dysfunction index.

The results showed that, 84% of the patients became symptoms free or improved while; only 16% showed unchanged signs and symptoms after completion of the treatment protocol.
Paravertebral injections: techniques and indications [abstract]
Authors: Baeza-Noci Jose
Published: March 2018

The paravertebral injections were first referred in 1989 by Dr. Cesare Verga [1], an italian orthopedist. He used them to treat disc herniation. We call them “classical paravertebral injections”. Later on, one colleague of him, Dr. Scuccimarra [2], used longer needles to inject ozone close to the foramen, under the hypothesis of improving the results, and he succeeded. They are known as “deep paravertebral injections”. Other techniques have been developed in order to improve the results, reduce the risks and shorten the treatment.[3-9]

The classical paravertebral approach is done locating the upper part of the spinous process of the superior vertebrae involved in the disco-radicular conflict and injecting 2,5 cm to the left and right of the spinous process with a 0,8 x 40 mm needle an amount of 5-10 mL per point depending on the size of the patient. Some authors(63) have proved that using lower ozone concentration (10 µg/mL) can be as useful as standard concentration (20 µg/mL). Our advice is to use a 0,4 x 40 mm needle or thinner if available. Local, topic anesthesia or cryotherapy can be used to reduce the pain of the needle. Injection should be done slowly. Using local anesthesia in the muscle can reduce the effect of ozone injection. The “deep paravertebral injection” uses a similar procedure, but the distance from the middle line is narrower (1,5 cm for cervical and dorsal injection and 2 cm for lumbar injection) and it is necessary using longer needles (0,4 or 0,5 x 90 mm spinal needle) to be able to locate the posterior joints with the tip of the needle an inject periarticularly. The amount of ozone used is the same.

It is also possible to inject over the laminae, close to the foramen, instead of around the facet joints, but risk of accidental dura or radicular puncture is greater (although without permanent side effects); this can be done for nerve root de-inflammation. Dr Verga modified his technique for cervical and dorsal disc herniation, narrowing the distance from the spinous process to 1,5 cm left and right, using shorter needles (25 or 30 mm) and decreasing the ozone volume per point to 3-7 mL. Dorsal approach uses the same technique as for cervical paravertebral injections.

The classical paravertebral injection produce a relaxation in the muscle spam of the lumbar spine in low back pain. The deep paravertebral injection produce an anti-inflammatory effect that can reduce inflammation on the facet joint or nerve root, depending on the point of injection.

Based on this empirical approach, and the publications that have already used them , the indications of these injections are:
-Disc herniation [1-2]
-Spondylolysis [10]
-Spondylosis [11-14]
-Lumbar spinal stenosis [7, 15]
-Symptomatic treatment of facet joint disease [7]
-Mechanical low back pain

These injections may have side effects due to the technique itself, not the ozone, but we have few reports on anecdotal cases, most of them without aftermath.
Ozone therapy in multiple disabled children [abstract]
Authors: Buch Rajeshwari
Published: March 2018

Multiple disabilities is term for child with several disabilities sensory associated with motor disabilities. Growing number of children are presented with development issues caused by cerebral palsy , autism, learning disabilities, ADHD, Genetic disorder, global delayed development. Problem of this children are multifold and affects the daily life of whole family No such conventional treatment can cure these problems these problems. They are treating specific symptoms of Autism and global delay improve the child functioning and helping them in daily activity . It is ethically correct to take advantage of ozone therapy when the best orthodox treatment has failed.

Anecdotal study was done in 47 children ranging from 8 months to 12 yrs of age with multiple disabilities. Along with conventional treatment and various therapy like occupational therapy, physio therapy, sensory integration, speech therapy, ozone therapy i.e rectal insufflation (10mcg to 80mcg per kg) ear insufflation (10mcg / 120ml for 5 to 10 mins ) were given. In selected cases S/C injection, vacuum massage, matra basti with Ayurvedic medicated ghrut like “Bhrami Ghruth, Ashwagandha Ghruth, Mahakalyank Ghruth”. The rectal insufflation of ozone is a systemic routed which dissolves quickly in the luminal contents of the bowel, where mucoprotiens and other secretory products with antioxidants activity readily react with ozone to produce reactive oxygen species (ROS) and lipid peroxidation products. These compound penetrate the muscular mucosa and enter the circulation of venous and lymphatic capillaries. This non-invasive technique can be used without risk in pediatric and elderly patients. Ear insufflation through capillaries of ear: it is believed that it is absorbed, it secretes hormone endorphin which gives cool and calm effect which improves the quality of sleep. Vacuum increases the blood flow and ozone can react better and s/c injection improves local blood circulation. Herbal drugs like centella acitica (Brahmi) shows significant improvement in neuron function in areas of the brain associated with learning and memory useful in ADHD. “Withenia Somnifera (Ashwagandh)” shows high affinity for GABA receptors that is helpful in memory loss, anxiety and ADHD “Ghrut” is a detoxifying agent makes the organ soft increases intelligence refine the intellect (dhi), improve the memory (Smruti), increases digestive AGNI ,cools the body and gives synergistic effect with ozone application (Yogwahi).

Observation.
1. Improvement in alertness, immunity, digestion
2. Weight gain
3. Improvement in muscle tone that enables child to perform daily physical activity

It is very early to arrive at any conclusion, but results are encouraging and very safe.
Role of ozone Disc Nucleolysis in Herniated intervertebral Disc: The Indian experience [abstract]
Authors: Ghatge Sharad
Published: March 2018

Aim.
To study the role of ozone disc nucleolysis in herniated intervertebral disc.

Materials.
We retrospectively analysed consecutives patients undergoing ozone disc nucleolysis. There were Total of 5265 patients with 2740 males and 2525 females. Their Age was ranging from 17 to 84 years. Patients weight was ranging from 36 to 138 kilograms. All the patients had failed conservative management for at least 3weeks.

Methods.
All the patients were clinically evaluated as per inclusion and exclusion criterions. Pre-treatment Visual Analogue scale (VAS)and Oswestry Disability Index (ODI).All the patients undergone Pre-treatments work up of MRI of the spine.256 patients were undergone Nerve physiological study (EMG,NCV and SSEP). All the procedures were carried out in Cathlab (Siemen's Artis zee) under local anaesthesia. In Lumbar region 3 cc of ozone and in cervical 1cc of ozone-oxygen mixture injected. In Lumbar region additionally 7 cc of ozone-oxygen mixture along with local anaesthetic (Lidocaine) - corticosteroid (Hydrocortisone and triamcinolone) - rheological enzyme (hynidase) injected in epidural space. There were 4406 patients with Lumbar disc herniation and 861 patients with cervical disc herniation. Statistical Analysis was done by SAS online version 2.

Results.
Outcome was assessed by VAS score, Oswestry Disability Index and modified MacNab scale at 6 month, 12 months and 18 months The mean baseline VAS score was 9 which became 4.9 post ozone disc nucleolysis . Disability Index was 36.13 at baseline which improved to 9.86 post treatment. Success rate was 81% by Modified MacNab outcome scale. Results by all above mentioned the three methods were found to be statistically significant.

Conclusion.
Ozone disc nucleolysis is highly effective in the treatment of herniated intervertebral disc . It is safe, Durable and cost effective in our series. Our results were slightly better than the published literature. However randomized control trial is recommended to further substantiate our results.
Neurological deficits treated with ozone therapy [abstract]
Authors: Kakkad Vipul J.
Published: March 2018

Patients and methods.
A total number of 35 patients with neurological déficits, like cerebrovascular accident (CVA), trigeminal neuralgia, post herpetic neuralgia, meningitis, cervical myelopathy, demyelinating disease, acoustic neuroma, muscular neuropathy and parkinsonism were treated with ozone therapy in our institution.

Results.
Group 1) Hemorrhagic - 6 patients
Group 2) Ischemic - 29 Patients

Both the groups showed improvement varying from 20% to 100%:
• Hemorrhagic patients took more time for improvement as compare to ischemic group.
• Hemorrhagic patients showed partial recovery.
• Ischemic group showed almost 90% - 100% recovery.

Patients who received ozone therapy after 12 months of onset could recover partially.

Route of ozone administration:
• 90 % Patients were given ozonized water, ear insufflation and rectal insufflation.
• Remaining 10% were treated with Major auto Haemotherapy or IV ozone saline, with ear insufflation.

Conclusions.
Even 3 to 8 years-old onset of CVA patients were benefited with ozone therapy. Cases of trigeminal neuralgia, post herpetic neuralgia, meningitis, cervical myelopathy, demyelinating of nerves, almost 100% cure rate.
Remaining cases like acoustic neuroma, muscular neuropathy, parkinsonism shown satisfactory improvement mild to moderate type.
Ozone Therapy in Integrated Cancer Management [abstract]
Authors: Sreekumar A.
Published: March 2018

Cancer has become one of the most dreadful life style disease causing morbidity and mortality globally and India is topping the world. But the real fact is “Cancer is one of the most curable diseases in this world today”. In 2007 over 12 million new cases were diagnosed across the planet and approximately 7.6 million cancer deaths occurred. By 2050, these numbers will rise to an expected 27 million new cases and 17.5 million cancer deaths if our ability to prevent, diagnose and treat cancer does not improve. Cancer is already the leading cause of death in Canada, Australia, and it's number 2 in Great Britain. In 5 years cancer is expected to be the leading cause of death in USA.

For more than 10 years we at Wellness Solutions have embraced rational cancer therapies proven both effective and minimally damaging to normal tissues and thus we have encouraged synergism between restorative therapies like Nutritional therapy, Ozone therapy and restrained Conventional Cancer therapy. We have found the best results in the combination of Personalized health assessment, Ozone therapy, parenteral nutrients, Oral nutrients and other Conventional management.

We use simultaneous high dose of Vitamin C, Major Auto Haemo Therapy (MAHT) for more than hundreds of cases with much better results than any modes of conventional and alternate therapy. High doses of Vitamin C with Ozone therapy have given better results than any chemotherapy protocols. Dr. Otto Warburg (1931 - 1944) proposed the link between Oxygen and Cancer. Various researches have spent millions of dollars searching for causes of cancer and has reached nowhere. According to Dr. Warburg search for carcinogen is useless without correcting the oxygen deprivation. 60% deprivation of oxygen in a cell will cause stress leading to disturbance in oxidation/reduction and uncontrolled cell division. We have experiences in more than 100 cancer cases in the last two years.

So, in integrated approach for cancer management Ozone therapy has a very prominent and decisive role for the maintenance of high quality of health and life.
Ozone Therapy in Gynecology [abstract]
Authors: Bande Sunayna
Published: March 2018

Explanation.
Gynecology is a vast branch including study of many disorders related to female genital tract, menstrual cycle, female hormones, infertility etc. Ozone Therapy is helpful in many of these.

PID - Pelvic Inflammatory Disease is a very common gynecological disorder caused by bacterial, fungal or other infections of genital tract and the pelvic area surrounding that. Ozone being an excellent antibacterial, antimicrobial and antifungal agent is very useful in treatment of PID. Ozone also gives very good results in cases of chronic PID which are resistant to many antibiotics, in such conditions, ozone gives better relief and also for longer time.

INFERTILITY. When a female is unable to conceive after 1 year of married life without using any contraception, she is considered for investigations and treatment of infertility. Many causes of infertility can be divided into:
- 1. Male factor
- 2. Female Factor
Female factor infertility – Infertility arising out many female factors, Ovarian and Tubal factors respond well to ozone therapy.
Tubal block due to inflammation may be treated with ozone therapy. Ozone given Vaginally can reduce the inflammation at the cornue or in tubes and open the block.
Ovarian factors are complex to treat. Problems like Anovulation, Ovarian cyst, Polycystic Ovaries can be treated with ozone therapy. Encouraging results have been found with Ozone therapy in PCOS with infertility. In my presentations, I am going to present a few cases of above conditions.
Percutaneous direct ozone injection in solid tumours [abstract]
Authors: Saxena Praveen K.
Published: March 2018

This lecture defines the scope of Direct Ozone Injection into solid tumors, and introduces attendees to the ways in which Direct Ozone can be given under ULTRASOUND guidance (USG).

Percutaneous USG guided Ozone injection is an exciting approach to destroying inoperable primary tumors or metastases in the liver in the treatment of hepatocellular (HCC), fewer than 40% of patients are candidates for surgery, and the rate of recurrence after curative surgery is high. Percutaneous techniques like Ozone Absolute alcohol & RFA are widely used for metastatic and small primary tumors.

It serves as a bridge for transplant candidates, especially in relation to small primary lesions.

Percutaneous Ozone is a minimally invasive, repeatable procedure with few complications. It is performed under Ultrasound guidance. Ozone results in a higher rate of complete necrosis and requires fewer treatment sessions than percutaneous ethanol injection (PEI). Long-term survival rates are also better. Our series of 4 patients compared well than the patients treated with Trans catheter arterial chemoembolization (TACE)

Cholangiocarcinoma’s with obstructive biliary system responded well with direct USG Guided Ozone Injections.
The long and winding road.
Authors: Baeza Noci Jose
Published: March 2018

It has been a very long trip since I wrote my last editorial. The first issue of the Journal received a strong welcome from all over the world in the beginning of 2016, but there was something missing that drove us to a winding road that ended in an even stronger situation: the support of the University of Valencia’s Publication Service (UVPS). The missing thing in the first issue was the DOI (Digital Object Identifier).

All the online journals have a DOI to identify the papers and allow a permanent link to them. Each DOI is unique for each paper. We look for a DOI provider in our environment and found the UVPS to offer DOIs for free to their teachers. As I am one of them, I went to ask for the requirements and the first one was to have an Open Journal; I emailed the WFOT Board and all agreed to change our journal policy to an Open one. Then, the University offered us to use their Open Journal System (OJS) of edition for free. This was a hard decision to take, because we have spent many hours developing our own free edition system, but the UVPS’s OJS offered something else … free indexation! So we decided to move the edition process to OJS.

It took us ONE YEAR to setup the OJS for our journal and when we finally got it ready, our journal web site (www.jo3t.org) was HACKED. We had then to change our domain name to the current one and use a secure protocol to access the journal (https), modify some security issues and move the contents of the old web site to the new one. Who says that the editorial work is boring?

Many papers for issue 2 had been sent using the old editorial manager, so we move them into the new OJS; some authors suffered this migration. Thanks for the patience!

Lately, is was impossible to get 10 papers for the second issue in 2017, so we decided to postpone the papers compiled for issue number 2 to issue number 3 and devote this second issue to be the Proceedings of our 5th WFOT Meeting in Mumbai – India. We upload all the abstracts to the system, that have been peer reviewed and language checked by our language copyeditor. When all this was done, the UVPS updated OJS and included a tool for NLM/Pubmed export that obliged us to re-review the papers for a proper indexation.

To finish our trip, we got our desired DOIs for our journal’s papers and we are ready for Pubmed indexing. The papers planned to be published now have been assigned to issue number 3, we want to publish this year in June-July. We have already papers for issues numbers 4 and 5 (2019) so I am happy to say that the Journal of Ozone Therapy is rolling on!!!

Again, thanks to every author for their patience. Now we start pushing for Pubmed indexation and have good Impact Factor. We have the tools and the help of the UVPS, but we need you all to get the journal we all need and want.
Host genetics and microbiome associations from the lens of genome wide association studies
Authors: Weissbrod Omer , Rothschild Daphna , Barkan Elad , Segal Eran
Published: March 2018

Recent studies indicate that the gut microbiome is partially heritable, motivating the need to investigate microbiome-host genome associations via microbial genome-wide association studies (mGWAS). Existing mGWAS demonstrate that microbiome-host genotypes associations are typically weak and are spread across multiple variants, similar to associations often observed in genome-wide association studies (GWAS) of complex traits. Here we reconsider mGWAS by viewing them through the lens of GWAS, and demonstrate that there are striking similarities between the challenges and pitfalls faced by the two study designs. We further advocate the mGWAS community to adopt three key lessons learned over the history of GWAS: (a) Adopting uniform data and reporting formats to facilitate replication and meta-analysis efforts; (b) enforcing stringent statistical criteria to reduce the number of false positive findings; and (c) considering the microbiome and the host genome as distinct entities, rather than studying different taxa and single nucleotide polymorphism (SNPs) separately. Finally, we anticipate that mGWAS sample sizes will have to increase by orders of magnitude to reproducibly associate the host genome with the gut microbiome.
Population diversity and relatedness in Sugarbirds (Promeropidae: Promerops spp.)
Authors: Haworth Evan S , Cunningham Michael J , Calf Tjorve Kathleen M.
Published: March 2018

Sugarbirds are a family of two socially-monogamous passerine species endemic to southern Africa. Cape and Gurney’s Sugarbird (Promerops cafer and P. gurneyi) differ in abundance, dispersion across their range and in the degree of sexual dimorphism in tail length, factors that affect breeding systems and potentially genetic diversity. According to recent data, P. gurneyi are in decline and revision of the species’ IUCN conservation status to a threatened category may be warranted. It is therefore necessary to understand genetic diversity and risk of inbreeding in this species. We used six polymorphic microsatellite markers and one mitochondrial gene (ND2) to compare genetic diversity in P. cafer from Cape Town and P. gurneyi from Golden Gate Highlands National Park, sites at the core of each species distribution. We describe novel universal avian primers which amplify the entire ND2 coding sequence across a broad range of bird orders. We observed high mitochondrial and microsatellite diversity in both sugarbird populations, with no detectable inbreeding and large effective population sizes.
Neurexin and Neuroligin-based adhesion complexes drive axonal arborisation growth independent of synaptic activity
Authors: Constance William D , Mukherjee Amrita , Fisher Yvette E , Pop Sinziana , Blanc Eric , Toyama Yusuke , Williams Darren W
Published: March 2018

Building arborisations of the right size and shape is fundamental for neural network function. Live imaging in vertebrate brains strongly suggests that nascent synapses are critical for branch growth during development. The molecular mechanisms underlying this are largely unknown. Here we present a novel system in Drosophila for studying the development of complex arborisations live, in vivo during metamorphosis. In growing arborisations we see branch dynamics and localisations of presynaptic proteins very similar to the ‘synaptotropic growth’ described in fish/frogs. These accumulations of presynaptic proteins do not appear to be presynaptic release sites and are not paired with neurotransmitter receptors. Knockdowns of either evoked or spontaneous neurotransmission do not impact arbor growth. Instead, we find that axonal branch growth is regulated by dynamic, focal localisations of Neurexin and Neuroligin. These adhesion complexes provide stability for filopodia by a ‘stick-and-grow’ based mechanism wholly independent of synaptic activity.
Mechanochemical coupling and bi-phasic force-velocity dependence in the ultra-fast ring ATPase SpoIIIE
Authors: Liu Ninning , Chistol Gheorghe , Cui Yuanbo , Bustamante Carlos
Published: March 2018

Multi-subunit ring-shaped ATPases are molecular motors that harness chemical free energy to perform vital mechanical tasks such as polypeptide translocation, DNA unwinding, and chromosome segregation. Previously we reported the intersubunit coordination and stepping behavior of the hexameric ring-shaped ATPase SpoIIIE (Liu et al., 2015). Here we use optical tweezers to characterize the motor’s mechanochemistry. Analysis of the motor response to external force at various nucleotide concentrations identifies phosphate release as the likely force-generating step. Analysis of SpoIIIE pausing indicates that pauses are off-pathway events. Characterization of SpoIIIE slipping behavior reveals that individual motor subunits engage DNA upon ATP binding. Furthermore, we find that SpoIIIE’s velocity exhibits an intriguing bi-phasic dependence on force. We hypothesize that this behavior is an adaptation of ultra-fast motors tasked with translocating DNA from which they must also remove DNA-bound protein roadblocks. Based on these results, we formulate a comprehensive mechanochemical model for SpoIIIE.
SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation
Authors: Sarikhani Mohsen , Mishra Sneha , Maity Sangeeta , Kotyada Chaithanya , Wolfgeher Donald , Gupta Mahesh P , Singh Mahavir , Sundaresan Nagalingam R
Published: March 2018

Glycogen synthase kinase 3 (GSK3) is a critical regulator of diverse cellular functions involved in the maintenance of structure and function. Enzymatic activity of GSK3 is inhibited by N-terminal serine phosphorylation. However, alternate post-translational mechanism(s) responsible for GSK3 inactivation are not characterized. Here, we report that GSK3α and GSK3β are acetylated at Lys246 and Lys183, respectively. Molecular modeling and/or molecular dynamics simulations indicate that acetylation of GSK3 isoforms would hinder both the adenosine binding and prevent stable interactions of the negatively charged phosphates. We found that SIRT2 deacetylates GSK3β, and thus enhances its binding to ATP. Interestingly, the reduced activity of GSK3β is associated with lysine acetylation, but not with phosphorylation at Ser9 in hearts of SIRT2-deficient mice. Moreover, GSK3 is required for the anti-hypertrophic function of SIRT2 in cardiomyocytes. Overall, our study identified lysine acetylation as a novel post-translational modification regulating GSK3 activity.