bluuebunny/crossref_metadata_2025 huggingface dataset:
Design and Fabrication of a Novel Window-Type Convection Device
Authors: Lin Han-Tang , Guu Yunn-Horng , Hsu Wei-Hsuan
Published: December 2020

Global warming, climate change, and ever-increasing energy demand are among the pressing challenges currently facing humanity. Particularly, indoor air conditioning, a major source of energy consumption, requires immediate improvement to prevent energy crises. In this study, various airfoil profiles were applied to create a window-type convection device that entrains air to improve convection between indoor and outdoor airflows and adjust the indoor temperature. How the geometric structure of the convection device affects its air entrainment performance was investigated on the basis of various airfoil profiles and outlet slit sizes of the airflow multiplier. The airfoil profiles were designed according to the 4-digit series developed by the National Advisory Committee for Aeronautics. The results revealed that airfoil thickness, airfoil camber, and air outlet slit size affected the mass flow rate of the convection device. Overall, the mass flow rate at the outlet of the convection device was more than 10 times greater than at the inlet, demonstrating the potential of the device to improve air convection. To validate these simulated results, the wind-deflector plate was processed using the NACA4424 airfoil with a 1.2 mm slit, and various operating voltages were applied to the convection device to measure the resulting wind speeds and calculate the corresponding mass flow rates. The experimental and simulated results were similar, with a mean error of <7%, indicating that the airfoil-shaped wind-deflector plate substantially improved air entrainment of the convection device to the goal of reduced energy consumption and carbon emissions.
Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate
Authors: Slavík Jan , Čmiel Vratislav , Hubálek Jaromír , Yang Yi , Ren Tian-Ling
Published: December 2020

Alignment and patterning of neurons have great importance in some research fields, especially for regenerative medicine and for the formation of artificial neural networks. Alignment of neurons on quartz grooves and parylene cues on quartz substrate was evaluated in this work. The neurons’ alignment on quartz grooves is considered to be topographical alignment, while the neurons’ alignment on parylene cues on quartz substrate is considered to be chemical alignment. Both quartz grooves’ and parylene cues’ widths were fabricated in a range from 2 µm to 8 µm; quartz grooves’ heights were in a range from 0.25 µm to 4 µm, while parylene cues’ heights were only 0.25 µm. Neurons were dissociated hippocampal neurons from rat E18. Neurons were cultivated on test substrates for 7 days before alignment evaluation. As expected, neurons aligned according to the direction of grooves and cues; however, transversal growth direction was also observed with much smaller tendency. Chemical alignment was found to be more effective than topographical alignment. If parylene cues are thin and distanced enough, then neurons have a tendency to follow the direction of individual parylene cues; however, neurons on quartz grooves have a tendency just to follow a preferable direction than individual quartz grooves.
Reference Data by Player Position for an Ice Hockey-Specific Complex Test
Authors: Schulze Stephan , Laudner Kevin G. , Delank Karl-Stefan , Brill Richard , Schwesig René
Published: December 2020

The aim of this study was to investigate position-specific (forwards, defenders) reference data for a scientifically evaluated (validity, reliability) ice hockey-specific complex test (IHCT). One hundred and four 3rd league professional ice hockey field players (mean ± standard deviations (SD); age: 26.4 ± 5.62 years) volunteered for the investigation. Players were categorized as forwards (n = 64) and defenders (n = 40). Data from the IHCT were collected over six seasons from three 3rd league teams. The IHCT included parameters for load (e.g., 10 m and 30 m skate times, transition and weave agility times with and without a puck, slap and wrist shots on goal) and stress (e.g., lactate, heart rate). The only significant (p < 0.002) difference between forwards and defenders for performance were found for weave agility with puck (p < 0.001). Forwards showed a higher average performance in this parameter than defenders. Differences were also found in weave agility without a puck (p = 0.008), 30 m backward sprinting without puck (p = 0.012) and goals after test (p = 0.030). This study provides position-specific reference data for a valid and reliable ice hockey-specific complex test for the 3rd league. These results may be used by coaches to judge player performance based on position (forwards vs. defenders). Moreover, coaches may use these data to evaluate the effectiveness of the most recent training period. Further research should extend this database to 1st and 2nd league players in order to enhance the scope of the test.
Build Orientation Optimization Based on Weighted Analysis of Local Surface Region Curvature
Authors: Guo Hongshuai , Xu Jinghua , Zhang Shuyou , Yi Guodong
Published: December 2020

Build orientation becomes a hot issue in 3D printing, which has a significant impact on the surface quality, support structure and final cost of the fabricated model. In this paper, we focus our attention on the surface quality of the part when fabricated by a 3D printer under certain specific build orientation. A novel method using curvature shift is proposed to search local regions with obvious curvature changes of the model printed. Based on curvature shift strategy, the model is divided into separate regions which can be treated differently. Every triangle facet of the regions is given different weight factor by calculating the average curvature of each region. Weighted facets is introduced in building volume error function. Candidate build orientation can be obtained by the eigenvalue decomposition and the optimal build orientation is the one which leads to the minimal error of the function. From the case study, the method proposed gets a smaller error with a lower printing cost and time, which means the model surface in higher weighted regions gets a higher surface quality when printed.
Impacts of the Tree Canopy and Chemical Reactions on the Dispersion of Reactive Pollutants in Street Canyons
Authors: Gonzalez Olivardia Franchesca G. , Matsuo Tomohito , Shimadera Hikari , Kondo Akira
Published: December 2020

Traffic-related air pollution in street canyons can cause health problems for pedestrians. In order to clarify the behavior of reactive pollutants, such as NOx and O3, in street canyons, a computational fluid dynamics (CFD) model coupled with a chemistry model and tree canopy model was developed, and then, a set of numerical experiments were performed to investigate the impacts of chemical reactions and aerodynamic effects of trees planted in a canyon. The results were compared with the observation data. Through the results of the numerical experiments designed to simulate a realistic urban street canyon, it was found that chemical reactions have a dominant impact on the NO/NO2 ratio and O3 concentration. While the tree canopy had little impact on the NO/NO2 ratio, it had a moderate impact on the flow field in the canyon and the amount of NOx and O3 in the canyon. In accordance with the aerodynamic effects of tree canopies, the local NOx concentration in the experiments increased and decreased by up to 51% and 11%, respectively. The current findings of this study demonstrate the utility of the proposed model for conducting air quality investigations in urban areas.
Direct Measurement of Mercury Deposition at Rural and Suburban Sites in Washington State, USA
Authors: Beutel Marc W. , DeSilva Lanka , Amegbletor Louis
Published: December 2020

Because of mercury’s (Hg) capacity for long-range transport in the atmosphere, and its tendency to bioaccumulate in aquatic biota, there is a critical need to measure spatial and temporal patterns of Hg atmospheric deposition. Dry deposition of Hg is commonly calculated as the product of a measured atmospheric concentration and an assumed deposition velocity. An alternative is to directly assess Hg deposition via accumulation on surrogate surfaces. Using a direct measurement approach, this study quantified Hg deposition at a rural site (Pullman) and suburban site (Puyallup) in Washington State using simple, low-cost equipment. Dry deposition was measured using an aerodynamic “wet sampler” consisting of a Teflon plate, 35 cm in diameter, holding a thin layer (2.5 mm) of recirculating acidic aqueous receiving solution. In addition, wet Hg deposition was measured using a borosilicate glass funnel with a 20-cm-diameter opening and a 1 L Teflon sampling bottle. Hg deposition was estimated based on changes in total Hg in the aqueous phase of the samplers. Dry Hg deposition was 2.4 ± 1.4 ng/m2·h (average plus/minus standard deviation; n = 4) in Pullman and 1.3 ± 0.3 ng/m2·h (n = 6) in Puyallup. Wet Hg deposition was 7.0 ± 4.8 ng/m2·h (n = 4) in Pullman and 1.1 ± 0.2 ng/m2·h (n = 3) in Puyallup. Relatively high rates of Hg deposition in Pullman were attributed to regional agricultural activities that enhance mercury re-emission and deposition including agricultural harvesting and field burning. Hg concentration in precipitation negatively correlated with precipitation depth, indicating that Hg was scavenged from the atmosphere during the beginning of storm events. Because of their relative simplicity and robustness, direct measurement approaches such as those described in this study are useful in assessing Hg deposition, and for comparing results to less direct estimates and model estimates of Hg deposition.
Incremental Capacity Analysis as a State of Health Estimation Method for Lithium-Ion Battery Modules with Series-Connected Cells
Authors: Krupp Amelie , Ferg Ernst , Schuldt Frank , Derendorf Karen , Agert Carsten
Published: December 2020

Incremental capacity analysis (ICA) has proven to be an effective tool for determining the state of health (SOH) of Li-ion cells under laboratory conditions. This paper deals with an outstanding challenge of applying ICA in practice: the evaluation of battery series connections. The study uses experimental aging and characterization data of lithium iron phosphate (LFP) cells down to 53% SOH. The evaluability of battery series connections using ICA is confirmed by analytical and experimental considerations for cells of the same SOH. For cells of different SOH, a method for identifying non-uniform aging states on the modules’ IC curve is presented. The findings enable the classification of battery modules with series and parallel connections based on partial terminal data.
Plant–Microbe Interaction
Authors: Dolatabadian Aria
Published: December 2020

Plant–microbe interaction is a complex, dynamic and continuous process that is as old as plant colonization on Earth [...]
Prophylactic Treatment with Vitamins C and B2 for Methotrexate-Induced Gastrointestinal Mucositis
Authors: da Silva Ferreira Ana Rita , Wardill Hannah R. , Havinga Rick , Tissing Wim J. E. , Harmsen Hermie J. M.
Published: December 2020

Mucositis is a common side-effect of chemotherapy treatment, inducing alterations in the composition of the gut microbiota. Redox active compounds, such as vitamins B2 and C, have been shown to reduce inflammation and enhance the growth of anaerobic bacteria in the gut. We therefore aimed to (1) validate the ability of these compounds to promote bacterial cell growth in vitro, and (2) determine their prophylactic efficacy in a rat model of methotrexate (MTX)-induced mucositis. Bacterial growth curves were performed to assess the growth kinetics of bacteria exposed to Vitamins C and B2 (0.5 mM). Male wistar rats (150–200 g) received vitamins B2 (12 mg/day) and C (50 mg/day) via daily oral gavage (from day −1 to day 10). MTX (45 mg/Kg) was administrated via I.V. injection (N = 4–8/group) on day 0. Body weight, water/food consumption and diarrhea were assessed daily. Blood and faecal samples were collected longitudinally to assess citrulline levels (mucositis biomarker) and gut microbiota composition. Vitamins C/B2 enhanced the in vitro growth of anaerobic bacteria Blautia coccoides and Roseburia intestinalis. Contrarily to vitamin B2, in vivo administration of Vitamin C significantly attenuated clinical symptoms of mucositis. Despite their influence on the composition of the gut microbiota, both vitamins did not modulate the course of MTX-induced mucositis, as accessed by plasma citrulline. Vitamins B2 and C enhanced anaerobic bacterial growth in vitro, however their ability to mitigate MTX-induced mucositis was limited.
Sleep Duration in Mouse Models of Neurodevelopmental Disorders
Authors: Saré Rachel Michelle , Lemons Abigail , Song Alex , Smith Carolyn Beebe
Published: December 2020

Sleep abnormalities are common in patients with neurodevelopmental disorders, and it is thought that deficits in sleep may contribute to the unfolding of symptoms in these disorders. Appreciating sleep abnormalities in neurodevelopmental disorders could be important for designing a treatment for these disorders. We studied sleep duration in three mouse models by means of home-cage monitoring: Tsc2+/− (tuberous sclerosis complex), oxytocin receptor (Oxtr) knockout (KO) (autism spectrum disorders), and Shank3 e4-9 KO (Phelan–McDermid syndrome). We studied both male and female mice, and data were analyzed to examine effects of both genotype and sex. In general, we found that female mice slept less than males regardless of genotype or phase. We did not find any differences in sleep duration in either Tsc2+/− or Oxtr KO mice, compared to controls. In Shank3 e4-9 KO mice, we found a statistically significant genotype x phase interaction (p = 0.002) with a trend that Shank3e4-9 KO mice regardless of sex slept more than control mice in the active phase. Our results have implications for the management of patients with Phelan–McDermid syndrome.
The Predictive Role of Prostate-Specific Antigen Changes Following Transurethral Resection of the Prostate for Patients with Localized Prostate Cancer
Authors: Wu Chun-Te , Huang Yun-Ching , Chen Wen-Cheng , Chen Miao-Fen
Published: December 2020

Regarding localized prostate cancer (PC), questions remain regarding which patients are appropriate candidates for conservative management. Some localized PC was an incidental finding in patients who received transurethral resection of the prostate (TURP) for urinary symptoms. It is known that TURP usually affects the level of prostate-specific antigen (PSA). In the present study, we examined whether changes in PSA levels after TURP possess a predictive value for localized PC. We retrospectively reviewed the clinical data of 846 early-stage PC patients who underwent TURP for urinary symptoms upon diagnosis at our hospital. Of 846 patients, 687 had tumor involvement in TURP specimens, and 362 had post-TURP PSA assessment. Our data revealed that, in addition to low GS and PSA levels at diagnosis, ≤5% tumor involvement in TURP specimens, greater PSA reduction (≥68%) following TURP, and post-TURP PSA ≤ 4 were significantly associated with better progression-free survival (PFS). Survival analysis revealed that the addition of prostate-directed local therapy significantly improved PFS in intermediate- and high-risk groups, but not in the low-risk group. Moreover, in the intermediate-risk group, local therapy improved PFS only for patients who were associated with post-TURP PSA > 4 ng/mL or <68% PSA reduction following TURP. We also found that local therapy had no obvious improvement in PFS for those with post-TURP ≤ 4 ng/mL regardless of pre-TURP PSA. In conclusion, conservative management is considered for patients at low or intermediate risk who have greater PSA reduction following TURP and low post-TURP PSA. Therefore, the levels of PSA following TURP might be helpful for risk stratification and the selection of patients for conservative management.
Daratumumab and Nanobody-Based Heavy Chain Antibodies Inhibit the ADPR Cyclase but not the NAD+ Hydrolase Activity of CD38-Expressing Multiple Myeloma Cells
Authors: Baum Natalie , Fliegert Ralf , Bauche Andreas , Hambach Julia , Menzel Stephan , Haag Friedrich , Bannas Peter , Koch-Nolte Friedrich
Published: December 2020

The nucleotides ATP and NAD+ are released from stressed cells as endogenous danger signals. Ecto-enzymes in the tumor microenvironment hydrolyze these inflammatory nucleotides to immunosuppressive adenosine, thereby, hampering anti-tumor immune responses. The NAD+ hydrolase CD38 is expressed at high levels on the cell surface of multiple myeloma (MM) cells. Daratumumab, a CD38-specific monoclonal antibody promotes cytotoxicity against MM cells. With long CDR3 loops, nanobodies and nanobody-based heavy chain antibodies (hcAbs) might bind to cavities on CD38 and thereby inhibit its enzyme activity more potently than conventional antibodies. The goal of our study was to establish assays for monitoring the enzymatic activities of CD38 on the cell surface of tumor cells and to assess the effects of CD38-specific antibodies on these activities. We monitored the enzymatic activity of CD38-expressing MM and other tumor cell lines, using fluorometric and HPLC assays. Our results showed that daratumumab and hcAb MU1067 inhibit the ADPR cyclase but not the NAD+ hydrolase activity of CD38-expressing MM cells. We conclude that neither clinically approved daratumumab nor recently developed nanobody-derived hcAbs provide a second mode of action against MM cells. Thus, there remains a quest for “double action” CD38-inhibitory antibodies.
The ITIM-Containing Receptor: Leukocyte-Associated Immunoglobulin-Like Receptor-1 (LAIR-1) Modulates Immune Response and Confers Poor Prognosis in Invasive Breast Carcinoma
Authors: Joseph Chitra , Alsaleem Mansour A. , Toss Michael S. , Kariri Yousif A. , Althobiti Maryam , Alsaeed Sami , Aljohani Abrar I. , Narasimha Pavan L. , Mongan Nigel P. , Green Andrew R. , Rakha Emad A.
Published: December 2020

Background: The leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1) plays a role in immune response homeostasis, extracellular matrix remodelling and it is overexpressed in many high-grade cancers. This study aimed to elucidate the biological and prognostic role of LAIR-1 in invasive breast cancer (BC). Methods: The biological and prognostic effect of LAIR-1 was evaluated at the mRNA and protein levels using well-characterised multiple BC cohorts. Related signalling pathways were evaluated using in silico differential gene expression and siRNA knockdown were used for functional analyses. Results: High LAIR-1 expression either in mRNA or protein levels were associated with high tumour grade, poor Nottingham Prognostic Index, hormone receptor negativity, immune cell infiltrates and extracellular matrix remodelling elements. High LAIR-1 protein expression was an independent predictor of shorter BC-specific survival and distant metastasis-free survival in the entire BC cohort and human epidermal growth factor receptor 2 (HER2)+ subtype. Pathway analysis highlights LAIR-1 association with extracellular matrix remodelling-receptor interaction, and cellular proliferation. Depletion of LAIR-1 using siRNA significantly reduced cell proliferation and invasion capability in HER2+ BC cell lines. Conclusion: High expression of LAIR-1 is associated with poor clinical outcome in BC. Association with immune cells and immune checkpoint markers warrant further studies to assess the underlying mechanistic roles.
Synergistic Analysis of Protein Corona and Haemoglobin Levels Detects Pancreatic Cancer
Authors: Caputo Damiano , Digiacomo Luca , Cascone Chiara , Pozzi Daniela , Palchetti Sara , Di Santo Riccardo , Quagliarini Erica , Coppola Roberto , Mahmoudi Morteza , Caracciolo Giulio
Published: December 2020

Simultaneous detection of multiple analytes from a single biological sample is gaining more attention in the development of more reliable and point-of-care diagnostic devices. We developed a multiplexed strategy that combined outcomes of clinical biomarkers with analysis of the protein corona that forms around graphene oxide sheets upon exposure to patient’s plasma. As a paradigmatic case study, we selected pancreatic ductal adenocarcinoma (PDAC), mainly because of the absence of effective detection strategies that resulted in an extremely low five-year survival rate after diagnosis (<10%). Association of protein corona analysis and haemoglobin levels discriminated PDAC patients from healthy volunteers in up to 90% of cases. If further confirmed in larger-cohort studies, this approach may be used in the detection of PDAC.
Atmospheric Pressure Plasma for Diesel Particulate Matter Treatment: A Review
Authors: Guo Xiurong , Ha Khanh Hop , Du Danfeng
Published: December 2020

The purification of diesel exhaust gas is of great importance to prevent the atmospheric emission of major pollutants such as diesel particulate matter and nitrogen oxides and meet the environmental regulations. The atmospheric-pressure plasma is attracting increasing interest and is a promising after-treatment technology for purifying diesel emission at low temperatures. However, when compared with the numerous publications on nitrogen oxides reduction by non-thermal plasma, using non-thermal plasma to particulate matter treatment have relatively limited. This work provides a comprehensive review of the plasma applications for diesel particulate matter treatment, including self-regenerating diesel particulate filter, diesel particulate matter removal, and simultaneous removal of diesel particulate matter and nitrogen oxides. The treatment of particulate matter from both simulated particulate matter sources and actual diesel engines also discussed in this comprehensive review. The challenge to this technology is limited energy consumption for plasma, which should be less than 5% (~30 J/L) of the overall fuel consumption. Until now, the atmospheric-pressure plasma has been no commercial implementation in diesel exhaust gas treatment, so more research is needed to be done in this field.
Recyclable Porous Glass-Ceramics from the Smelting of MSWI Bottom Ash
Authors: Rabelo Monich Patricia , Lucas Hugo , Friedrich Bernd , Bernardo Enrico
Published: December 2020

Material from the electric arc furnace smelting of municipal solid waste incineration (MSWI) bottom ash was easily converted into highly porous glass-ceramics by a combination of inorganic gel casting and sinter-crystallization at 1000 °C. In particular, the gelation of aqueous suspensions of fine glass powders, transformed into “green” foams by intensive mechanical stirring, occurred with a limited addition of alkali activator (1 M NaOH). The products coupled the stabilization of pollutants with good mechanical properties (e.g., compressive strength approaching 4 MPa). Interestingly, they could be used also as raw material for new glass-ceramic foams, obtained by the same gel casting and sintering method, with no degradation of chemical stability. Limitations in the crushing strength, derived from the limited viscous flow densification of semi-crystalline powders, were overcome by mixing powders from recycled foams with 30 wt% soda-lime glass. The new products finally featured an even higher strength-to-density ratio than the foams from the first cycle.
First-Principles Electronic-Structure Study of Graphene Decorated with 4d-Transition Atoms
Authors: Hu Ran , Zhang Wei-Chao , Sun Wei-Feng
Published: December 2020

Adsorption configurations, electronic structures and net spins of graphene adsorbing 4d transition atoms are calculated by first-principles calculations to explore the magnetic modification of decorating metal atoms on graphene. Y, Zr and Nb atoms can be adsorbed on graphene sheet via ionic bonds with an evident charge transfer, while Mo, Tc, Ru and Rh atoms form covalent-like bonding with graphene carbon atoms due to orbital hybridization, as indicated by Mulliken atomic charges and electron density differences. The 4d-transition atoms can be adsorbed on a carbon-ring center and atomic-bridge with a high binding energy as the typical chemisorption, which leads to specific modifications in electronic-band character and magnetic properties by introducing electron-states near Fermi-level. By adsorbing 4d-transition atoms, the electronic structure of graphene will alter from a semi-metal to a metal character, and engender net spin magnetism from the spin-polarization in 5s and 4d orbitals of adsorption atoms. This paper provides a significant theoretical basis for further experimental explorations of the atom-decorated graphene in nanoelectronics.
Development and Validation of an Automated Radiomic CT Signature for Detecting COVID-19
Authors: Guiot Julien , Vaidyanathan Akshayaa , Deprez Louis , Zerka Fadila , Danthine Denis , Frix Anne-Noëlle , Thys Marie , Henket Monique , Canivet Gregory , Mathieu Stephane , Eftaxia Evanthia , Lambin Philippe , Tsoutzidis Nathan , Miraglio Benjamin , Walsh Sean , Moutschen Michel , Louis Renaud , Meunier Paul , Vos Wim , Leijenaar Ralph T. H. , Lovinfosse Pierre
Published: December 2020

The coronavirus disease 2019 (COVID-19) outbreak has reached pandemic status. Drastic measures of social distancing are enforced in society and healthcare systems are being pushed to and beyond their limits. To help in the fight against this threat on human health, a fully automated AI framework was developed to extract radiomics features from volumetric chest computed tomography (CT) exams. The detection model was developed on a dataset of 1381 patients (181 COVID-19 patients plus 1200 non COVID control patients). A second, independent dataset of 197 RT-PCR confirmed COVID-19 patients and 500 control patients was used to assess the performance of the model. Diagnostic performance was assessed by the area under the receiver operating characteristic curve (AUC). The model had an AUC of 0.882 (95% CI: 0.851–0.913) in the independent test dataset (641 patients). The optimal decision threshold, considering the cost of false negatives twice as high as the cost of false positives, resulted in an accuracy of 85.18%, a sensitivity of 69.52%, a specificity of 91.63%, a negative predictive value (NPV) of 94.46% and a positive predictive value (PPV) of 59.44%. Benchmarked against RT-PCR confirmed cases of COVID-19, our AI framework can accurately differentiate COVID-19 from routine clinical conditions in a fully automated fashion. Thus, providing rapid accurate diagnosis in patients suspected of COVID-19 infection, facilitating the timely implementation of isolation procedures and early intervention.
In Vivo Measurement of Neurochemical Abnormalities in the Hippocampus in a Rat Model of Cuprizone-Induced Demyelination
Authors: Lee Do-Wan , Kwon Jae-Im , Woo Chul-Woong , Heo Hwon , Kim Kyung Won , Woo Dong-Cheol , Kim Jeong Kon , Lee Dong-Hoon
Published: December 2020

This study quantitatively measured the changes in metabolites in the hippocampal lesions of a rat model of cuprizone-induced demyelination as detected using in vivo 7 T proton magnetic resonance spectroscopy. Nineteen Sprague Dawley rats were randomly divided into two groups and fed a normal chow diet or cuprizone (0.2%, w/w) for 7 weeks. Demyelinated hippocampal lesions were quantitatively measured using a 7 T magnetic resonance imaging scanner. All proton spectra were quantified for metabolite concentrations and relative ratios. Compared to those in the controls, the cuprizone-induced rats had significantly higher concentrations of glutamate (p = 0.001), gamma-aminobutyric acid (p = 0.019), and glutamate + glutamine (p = 0.001); however, creatine + phosphocreatine (p = 0.006) and myo-inositol (p = 0.001) concentrations were lower. In addition, we found that the glutamine and glutamate complex/total creatine (p < 0.001), glutamate/total creatine (p < 0.001), and GABA/total creatine (p = 0.002) ratios were significantly higher in cuprizone-treated rats than in control rats. Our results showed that cuprizone-induced neuronal demyelination may influence the severe abnormal metabolism in hippocampal lesions, and these responses could be caused by microglial activation, mitochondrial dysfunction, and astrocytic necrosis.
Management of Dental Avulsion Injuries: A Survey of Dental Support Staff in Cairns, Australia
Authors: Abraham Yannis , Christy Roshini , Gomez-Kunicki Americo , Cheng Ting , Eskarous Silvia , Samaan Verona , Khan Ahsen , Sholapurkar Amar
Published: December 2020

Background/Aim: The aim of this study was to assess the knowledge of dental support staff in providing appropriate first-aid advice regarding dental avulsion emergencies. Methods: This study was reported according to the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines for cross-sectional studies. Dental support staff (includes dental assistants, administrative staff and other non-clinical staff) were contacted and data were collected from 50 private dental clinics across the Greater Cairns Area, Queensland, Australia. These data were collected through an online survey throughout 2020. Descriptive statistics and Pearson’s Chi-squared test was used to analyze the data and any associations between categorical outcomes. Results: This survey yielded a response rate of 34.1% with a margin of error of 10.3%. More than four-tenths of participants (42%) reported that they had received some form of dental avulsion management training previously. All but five participants (92%) denoted that they would immediately replant an avulsed permanent tooth. More than half of all participants would choose to rinse a soiled avulsed tooth with fresh milk (55%) and transport that tooth in fresh milk (65%) should they not be able to replant the tooth at the site. Almost nine in every ten participants (85%) expressed willingness to further their training in this area. Knowledge in replanting avulsed permanent teeth was found to be significantly impacted by gender, age, years of experience and participation in formal avulsion training. Male participants were found to be significantly more likely (p = 0.025) to replant a permanent avulsed tooth than their female counterparts. Participants who were 40 years of age and above were found to be significantly more likely to choose fresh milk to transport avulsed teeth (p = 0.0478). Older participants (p = 0.0021), alongside those who had greater years of experience (p = 0.0112) and those who had undertaken formal avulsion training (p = 0.0106) were all significantly more likely to express greater confidence in their ability to manage dental avulsion injuries. Participants who had previously received some form of education regarding avulsion injury management were also most likely to warrant further education and training in this area (p < 0.0001). Conclusion: This study demonstrated that dental support staff in the Greater Cairns Area seem to have a fair grasp of first-aid knowledge regarding the management of dental avulsion injuries. This result indicates that this knowledge has been picked up through years of experience, rather than a formal education. Despite this, one would expect people who work in the dental industry to be able to provide accurate and appropriate assistance during dental emergencies, hence, further training is warranted to ensure optimum patient outcomes.
Regularized Maximum Diversification Investment Strategy
Authors: Koné N’Golo
Published: December 2020

The maximum diversification has been shown in the literature to depend on the vector of asset volatilities and the inverse of the covariance matrix of the asset return. In practice, these two quantities need to be replaced by their sample statistics. The estimation error associated with the use of these sample statistics may be amplified due to (near) singularity of the covariance matrix, in financial markets with many assets. This, in turn, may lead to the selection of portfolios that are far from the optimal regarding standard portfolio performance measures of the financial market. To address this problem, we investigate three regularization techniques, including the ridge, the spectral cut-off, and the Landweber–Fridman approaches in order to stabilize the inverse of the covariance matrix. These regularization schemes involve a tuning parameter that needs to be chosen. In light of this fact, we propose a data-driven method for selecting the tuning parameter. We show that the selected portfolio by regularization is asymptotically efficient with respect to the diversification ratio. In empirical and Monte Carlo experiments, the resulting regularized rules are compared to several strategies, such as the most diversified portfolio, the target portfolio, the global minimum variance portfolio, and the naive 1/N strategy in terms of in-sample and out-of-sample Sharpe ratio performance, and it is shown that our method yields significant Sharpe ratio improvements.
Development of Frequency Weighted Model Reduction Algorithm with Error Bound: Application to Doubly Fed Induction Generator Based Wind Turbines for Power System
Authors: Bashir Sajid , Batool Sammana , Imran Muhammad , Imran Muhammad , Ahmad Mian Ilyas , Malik Fahad Mumtaz , Ali Usman
Published: December 2020

The state-space representations grant a convenient, compact, and elegant way to examine the induction and synchronous generator-based wind turbines, with facts readily available for stability, controllability, and observability analysis. The state-space models are used to look into the functionality of different wind turbine technologies to fulfill grid code requirements. This paper deals with the model order reduction of the Variable-Speed Wind Turbines model with the aid of improved stability preserving a balanced realization algorithm based on frequency weighting. The algorithm, which is in view of balanced realization based on frequency weighting, can be utilized for reducing the order of the system. Balanced realization based model design uses a full frequency spectrum to perform the model reduction. However, it is not possible practically to use the full frequency spectrum. The Variable-Speed Wind Turbines model utilized in this paper is stable and includes various input-output states. This brings a complicated state of affairs for analysis, control, and design of the full-scale system. The proposed work produces steady and precise outcomes such as in contrast to conventional reduction methods which shows the efficacy of the proposed algorithm.
Using GatorEye UAV-Borne LiDAR to Quantify the Spatial and Temporal Effects of a Prescribed Fire on Understory Height and Biomass in a Pine Savanna
Authors: Shrestha Maryada , Broadbent Eben N. , Vogel Jason G.
Published: December 2020

In the pine savannas of the southeastern United States, prescribed fire is commonly used to manipulate understory structure and composition. Understory characteristics have traditionally been monitored with field sampling; however, remote sensing could provide rapid, spatially explicit monitoring of understory dynamics. We contrasted pre- vs. post-fire understory characteristics collected with fixed area plots with estimates from high-density LiDAR point clouds collected using the unmanned aerial vehicle (UAV)-borne GatorEye system. Measuring within 1 × 1 m field plots (n = 20), we found average understory height ranged from 0.17–1.26 m and biomass from 0.26–4.86 Mg C ha−1 before the fire (May 2018), and five months after the fire (November 2018), height ranged from 0.11–1.09 m and biomass from 0.04–3.03 Mg C ha−1. Understory heights estimated with LiDAR were significantly correlated with plot height measurements (R2 = 0.576, p ≤ 0.001). Understory biomass was correlated with in situ heights (R2 = 0.579, p ≤ 0.001) and LiDAR heights (R2 = 0.507, p ≤ 0.001). The biomass estimates made with either height measurement did not differ for the measurement plots (p = 0.263). However, for the larger research area, the understory biomass estimated with the LiDAR indicated a smaller difference after the burn (~12.7% biomass reduction) than observed with in situ measurements (~16% biomass reduction). The two approaches likely differed because the research area’s spatial variability was not captured by the in-situ measurements (0.2% of the research area measured) versus the wall-to-wall coverage provided by LiDAR. The additional benefit of having spatially explicit measurements with LiDAR, and its ease of use, make it a promising tool for land managers wanting greater spatial and temporal resolution in tracking understory biomass and its response to prescribed fire.
Fertilization and Tree Species Influence on Stable Aggregates in Forest Soil
Authors: Kemner Jacob E. , Adams Mary Beth , McDonald Louis M. , Peterjohn William T. , Kelly Charlene N.
Published: December 2020

Background and objectives: aggregation and structure play key roles in the water-holding capacity and stability of soils and are important for the physical protection and storage of soil carbon (C). Forest soils are an important sink of ecosystem C, though the capacity to store C may be disrupted by the elevated atmospheric deposition of nitrogen (N) and sulfur (S) compounds by dispersion of soil aggregates via acidification or altered microbial activity. Furthermore, dominant tree species and the lability of litter they produce can influence aggregation processes. Materials and methods: we measured water-stable aggregate size distribution and aggregate-associated organic matter (OM) content in soils from two watersheds and beneath four hardwood species at the USDA Forest Service Fernow Experimental Forest in West Virginia, USA, where one watershed has received (NH4)2SO4 fertilizer since 1989 and one is a reference/control of similar stand age. Bulk soil OM, pH, and permanganate oxidizable carbon (POXC) were also measured. Research highlights: fertilized soil exhibited decreased macro-aggregate formation and a greater proportion of smaller micro-aggregates or unassociated clay minerals, particularly in the B-horizon. This shift in aggregation to soil more dominated by the smallest (<53 µm) fraction is associated with both acidification (soil pH) and increased microbially processed C (POXC) in fertilized soil. Intra-aggregate OM was also depleted in the fertilized soil (52% less OM in the 53–2000 µm fractions), most strongly in subsurface B-horizon soil. We also document that tree species can influence soil aggregation, as soil beneath species with more labile litter contained more OM in the micro-aggregate size class (<250 µm), especially in the fertilized watershed, while species with more recalcitrant litter promoted more OM in the macro-aggregate size classes (500–2000 µm) in the reference watershed. Conclusions: long-term fertilization, and likely historic atmospheric deposition, of forest soils has weakened macro-aggregation formation, with implications for soil stability, hydrology, and storage of belowground C.
Extraction and Analyses of Flavonoids and Phenolic Acids from Canadian Goldenrod and Giant Goldenrod
Authors: Zekič Jure , Vovk Irena , Glavnik Vesna
Published: December 2020

Invasive alien plant species Canadian goldenrod (Solidago canadensis L.) and giant goldenrod (Solidago gigantea Aiton) were investigated as a source of phytochemicals and yellow dyes. Flavonoids and phenolic acids were extracted from the inflorescence of Canadian goldenrod with thirteen extraction solvents ethanol, methanol, acetone, water, and mixtures of organic solvents (70%, 80%, and 90%) with water. High performance thin-layer chromatography (HPTLC) coupled to densitometry and high-performance liquid chromatography with photo-diode array detector (HPLC-PDA) were used for analyses of the obtained sample test solutions (STSs), which showed the best and comparable extraction efficiencies for 70% acetone(aq), 70% methanol(aq), and 70% ethanol(aq). HPTLC combined with image analyses in fluorescent mode resulted in different chromatographic fingerprints for Canadian goldenrod and giant goldenrod STSs (70% acetone(aq)) after development, after post-chromatographic derivatization with NP reagent and after use of PEG reagent. The developed HPLC methods enabled analyses of phenolic acids and flavonoids (aglycones and glycosylated) in STSs and hydrolyzed STSs form inflorescence of Canadian and giant goldenrod. Different contents of chlorogenic acid, rutin, hyperoside, isoquercetin, and quercetin were observed in STSs of both goldenrod species. The analyses of hydrolyzed STSs confirmed that glycosylated flavonoids in Canadian and giant goldenrod inflorescence are mainly glycosides of quercetin, kaempferol, and isorhamnetin. Additional analyses using HPTLC and HPLC coupled to tandem mass spectrometry (MS/MS; HPTLC-MS/MS and LC-MS/MS) enabled tentative identification of phenolic acids and flavonoids (10 with HPTLC-MS/MS and 15 with LC-MS/MS), from which several were identified in Canadian (4 with HPTLC-MS/MS and 8 with LC-MS/MS) and in giant (7 with HPTLC-MS/MS and 9 with LC-MS/MS) goldenrod for the first time.
Long-Term Impact of Suppressive Antibiotic Therapy on Intestinal Microbiota
Authors: Escudero-Sánchez Rosa , Ponce-Alonso Manuel , Barragán-Prada Hugo , Morosini María Isabel , Cantón Rafael , Cobo Javier , del Campo Rosa
Published: December 2020

The aim was to describe the safety of indefinite administration of antibiotics, the so-called suppressive antibiotic therapy (SAT) and to provide insight into their impact on gut microbiota. 17 patients with SAT were recruited, providing a fecal sample. Bacterial composition was determined by 16S rDNA massive sequencing, and their viability was explored by PCR-DGGE with and without propidium monoazide. Presence of antibiotic multirresistant bacteria was explored through the culture of feces in selective media. High intra-individual variability in the genera distribution regardless of the antibiotic or antibiotic administration ingestion period, with few statistically significant differences detected by Bray-Curtis distance-based principle component analysis, permutational multivariate analysis of variance and linear discriminant analysis effect size analysis. However, the microbiota composition of patients treated with both beta-lactams and sulfonamides clustered by a heat map. Curiously, the detection of antibiotic resistant bacteria was almost anecdotic and CTX-M-15-producing E. coli were detected in two subjects. Our work demonstrates the overall clinical safety of SAT and the low rate of the selection of multidrug-resistant bacteria triggered by this therapy. We also describe the composition of intestinal microbiota under the indefinite use of antibiotics for the first time.
Ethos in Early Chinese Rhetoric: The Case of “Heaven”
Authors: Wei Yong-Kang
Published: December 2020

Though applicable in many Western historical-cultural settings, the Aristotelian model of ethos is not universal. As early Chinese rhetoric shows in the example of cheng-yan or “ethos of sincereness,” inspiring trust does not necessarily involve a process of character-based self-projection. In the Aristotelian model, the rhetor stands as a signifier of ethos, with an ideology of individualism privileged, whereas Chinese rhetoric assumes a collectivist model in which ethos belongs, not to an individual or a text, but rather to culture and cultural tradition. This essay will be concentrating on the concept of Heaven, central to the cultural and institutional systems of early Chinese society, in an attempt to explore collective ethos as a function of cultural heritage. Heaven, it shall be argued, plays a key role in the creation of Chinese ethos. This essay will also contrast the logocentrism of Western rhetorical tradition with the ethnocentrism of Chinese tradition. The significance of Heaven in its role as a defining attribute of Chinese ethos is reflective of a unique cultural heritage shaped by a collective human desire in seeking a consciousness of unity with the universe. Just as there are historical, cultural, and philosophical reasons behind logocentrism in the West, so the ethnocentric turn of Chinese rhetoric should be appreciated in light of a cultural tradition that carries its own historical complexities and philosophical intricacies.
Twins/Zwillinge: A Broader View. A Contribution to Stith Thompson’s Incomplete Motif System—A Case of the Continuation of Pseudoscientific Fallacies †
Authors: El-Shamy Hasan M.
Published: December 2020

Explaining the rationale and main objectives for his motif system; Stith Thompson declared that it emulates what “the scientists have done with the worldwide phenomena of biology” (Thompson 1955, I, p. 10). In this respect; the underlying principles for motif identification and indexing are comparable to those devised by anthropologists at Yale for “categorizing” culture materials into 78 macro-units and 629 subdivisions thereof used to establish “The Human Relations Area Files” (HRAF). By comparison, 23 divisions (chapters) make up the spectrum of sociocultural materials covered in Thompson’s Motif-Index system. Thompson’s cardinal themes are divided into 1730 subdivisions permitting more specificity of identification (El-Shamy 1995, I, xiii). Historically; the disciplines of “anthropology” and of “folklore” targeted different categories of the human population; with “folklore” assigned to populations stratified into “social classes” (Dorson 1972, pp. 4–5: For details, see El-Shamy: “Folk Groups” (1997b, pp. 318–322, in: T.A Green, gen. ed. 1997c, p. 321); El-Shamy 1980, p. li; compare El-Shamy (1997a), p. 233 (“African hunter”). The limitations Thompson placed on the goals of his motif system (along with its tale-type companion) were triggered by the fact that “folklore” was; then; primarily interested in literature (prose and verse). The sociocultural milieu surrounding the creation of the literary forms occupied minor roles. Considering that a folktale is a “description of life and/or living” including all five universal culture institutions; the relevance of the contents of folktales are of primary significance for understanding the community in which they were born and maintained (El-Shamy 1995, I, p. xiii). Consequently; for the present writer; a folktale is considered a sixth (universal) culture institution. Also; because Thompson’s Motif-Index sought global coverage; many regions and national entities didn’t receive adequate attention: significant fields of human experience are missing or sketchily presented. This article offers two cases as examples of: (1) How editors of folklore publications ignore novel ideas incompatible with established trends; and (2) Samples of the spectrum of current psychosocial issues addressed in an expanded Thompson’s System (with more than 26,000 new motifs and 630 tale-types added).