bluuebunny/crossref_metadata_2025 huggingface dataset:
Two Distinct Faces of Vitamin C: AA vs. DHA
Authors: Ferrada Luciano , Magdalena Rocío , Barahona María Jose , Ramírez Eder , Sanzana Cristian , Gutiérrez José , Nualart Francisco
Published: February 2021

Historically, vitamin C has been associated with many regulatory processes that involve specific signaling pathways. Among the most studied signaling pathways are those involved in the regulation of aging, differentiation, neurotransmission, proliferation, and cell death processes in cancer. This wide variety of regulatory effects is due to the fact that vitamin C has a dual mechanism of action. On the one hand, it regulates the expression of genes associated with proliferation (Ccnf and Ccnb1), differentiation (Sox-2 and Oct-4), and cell death (RIPK1 and Bcl-2). At the same time, vitamin C can act as a regulator of kinases, such as MAPK and p38, or by controlling the activation of the NF-kB pathway, generating chronic responses related to changes in gene expression or acute responses associated with the regulation of signal transduction processes. To date, data from the literature show a permanent increase in processes regulated by vitamin C. In this review, we critically examine how vitamin C regulates these different cellular programs in normal and tumor cells.
Antioxidant-Based Therapies in Male Infertility: Do We Have Sufficient Evidence Supporting Their Effectiveness?
Authors: Amorini Angela Maria , Listorti Ilaria , Bilotta Gabriele , Pallisco Romina , Saab Miriam Wissam , Mangione Renata , Manca Benedetta , Lazzarino Giacomo , Tavazzi Barbara , Lazzarino Giuseppe , Bilotta Pasquale
Published: February 2021

Under physiological conditions, reactive oxygen species (ROS) play pivotal roles in various processes of human spermatozoa. Indeed, semen requires the intervention of ROS to accomplish different stages of its maturation. However, ROS overproduction is a well-documented phenomenon occurring in the semen of infertile males, potentially causing permanent oxidative damages to a vast number of biological molecules (proteins, nucleic acids, polyunsaturated fatty acids of biological membrane lipids), negatively affecting the functionality and vitality of spermatozoa. ROS overproduction may concomitantly occur to the excess generation of reactive nitrogen species (RNS), leading to oxidative/nitrosative stress and frequently encountered in various human pathologies. Under different conditions of male infertility, very frequently accompanied by morpho-functional anomalies in the sperm analysis, several studies have provided evidence for clear biochemical signs of damages to biomolecules caused by oxidative/nitrosative stress. In the last decades, various studies aimed to verify whether antioxidant-based therapies may be beneficial to treat male infertility have been carried out. This review analyzed the results of the studies published during the last ten years on the administration of low-molecular-weight antioxidants to treat male infertility in order to establish whether there is a sufficient number of data to justify antioxidant administration to infertile males. An analysis of the literature showed that only 30 clinical studies tested the effects of the administration of low-molecular-weight antioxidants (administered as a single antioxidant or as a combination of different antioxidants with the addition of vitamins and/or micronutrients) to infertile males. Of these studies, only 33.3% included pregnancy and/or live birth rates as an outcome measure to determine the effects of the therapy. Of these studies, only 4 were case–control studies, and only 2 of them found improvement of the pregnancy rate in the group of antioxidant-treated patients. Additionally, of the 30 studies considered in this review, only 43.3% were case–control studies, 66.7% enrolled a number of patients higher than 40, and 40% carried out the administration of a single antioxidant. Therefore, it appears that further studies are needed to clearly define the usefulness of antioxidant-based therapies to treat male infertility.
The Ameliorative Role of Acacia senegal Gum against the Oxidative Stress and Genotoxicity Induced by the Radiographic Contrast Medium (Ioxitalamate) in Albino Rats
Authors: El-Garawani Islam , Hassab El-Nabi Sobhy , El Kattan Ahmed , Sallam Azza , Elballat Sabha , Abou-Ghanima Shaimaa , El Azab Islam H. , R. El-Seedi Hesham , A. M. Khalifa Shaden , El-Shamy Sawsan
Published: February 2021

Arabic gum (Acacia senegal, AG) is proven effective antioxidant and cytoprotective agent. The present study was designed to test this notion by investigating the possible role of AG against the radiographic contrast medium (Ioxitalamate, Telebrix-35®, TBX)-induced oxidative stress and genotoxicity. Albino rats were divided into four groups and supplied with either; distilled water, daily 10% (w/v) AG, an intravenous dose of TBX (1600 mg I/kg b.wt) and co-administration of TBX and AG. Rats were sacrificed and blood samples were collected to assess the genotoxicity employing the peripheral blood leucocytes fluorescent double staining; namely the acridine orange/ethidium bromide (AO/EB) staining and alkaline comet assay. Further, chromosomal analyses were done in bone marrow cells. Serum urea and creatinine levels, in addition to malondialdehyde (MDA), nitric oxide (NO), catalase (CAT) and glutathione (GSH) levels in kidney tissues were measured. Liquid chromatography-mass spectrophotometry (LC-MS-MS) was performed to identify the chemical composition of AG extract. Kidney functions, single/double-stranded DNA damage, chromosomal aberrations, mitotic index, MDA and NO levels were significantly (p < 0.001) increased in TBX-treated group compared to the control and AG-treated one. Meanwhile, CAT and GSH activities were significantly diminished and the AG supplementation significantly (p < 0.001) ameliorated these effects compared with the control and AG-treated groups. Five compounds have been identified using GNPS networking including 7,3′,4′-Trihydroxyisoflavone, Noscapine, Tetrahydropapaveroline, Costunolide, Hesperidin. In conclusion, results of the present study suggest that AG exerted a protective role against TBX-induced oxidative stress and genotoxicity which may be attributed to the active metabolites in the gum.
Supercritical CO2 Extraction and Microencapsulation of Lycopene-Enriched Oleoresins from Tomato Peels: Evidence on Antiproliferative and Cytocompatibility Activities
Authors: Mihalcea Liliana , Crăciunescu Oana , Gheonea (Dima) Ionica , Prelipcean Ana-Maria , Enachi Elena , Barbu Vasilica , Bahrim Gabriela Elena , Râpeanu Gabriela , Oancea Anca , Stănciuc Nicoleta
Published: February 2021

Tomato peels are used as a valuable material to extract lycopene-rich oleoresins by supercritical CO2 extraction. The extraction involves continuous circling of CO2 to the extractor after removing the solute in the separators, S40 and S45, where the solvent power of the CO2 is reduced by reducing pressure down to 20 MPa in S40 and 5 MPa in S45, respectively, leading to two extracts. Lycopene is found to be the major compound, representing 93% and 76% of the total carotenoids in S40 and S45 extracts, respectively. The two extracts are microencapsulated in whey protein concentrate and acacia gum by complex coacervation and freeze-drying, leading to corresponding P40 and P45 powders, with antioxidant activity of 8.57 ± 0.74 and 9.37 ± 0.48 mMol TEAC/g DW in P40 and P45, respectively. Different structural and morphological patterns are observed, with finer microparticles of 1–2 µm in P45. Both powders show dose and time-dependent antiproliferative activity. The half-maximal inhibitory concentration values are 100 µg/mL for P40 and 750 µg/mL for P45 sample, indicating a higher antiproliferative effect of P40 over P45 in HT-29 cell culture. The powders have an extended range of cytocompatibility, up to 1000 µg/mL, in L929 normal cells, stimulating the cell growth. Lycopene retention is tested, and values of 48% and 29% in P40 and P45 are found after 21 days at 25 °C, with the degradation rate in P45 significantly higher, due to the higher content of the surface lycopene, which favored its degradation.
Carotenoids and Cognitive Outcomes: A Meta-Analysis of Randomized Intervention Trials
Authors: Davinelli Sergio , Ali Sawan , Solfrizzi Vincenzo , Scapagnini Giovanni , Corbi Graziamaria
Published: February 2021

Recent evidence suggests that diet can modify the risk of future cognitive impairment and dementia. A biologically plausible rationale and initial clinical data indicate that the antioxidant activities of dietary carotenoids may assist the preservation of cognitive function. A meta-analysis of randomized controlled trials was conducted to examine the relationship between carotenoid supplementation and cognitive performance. A literature search was conducted in the MEDLINE (via PubMed), Scopus, Web of Science, and Cochrane databases from their inception to July 2020. A total of 435 studies were retrieved. Abstract screening using predefined inclusion and exclusion criteria was followed by full-text screening and data extraction of study characteristics and measured outcomes. A meta-analysis of eligible trials was performed using a random-effects model to estimate pooled effect size. We identified 9 studies with a total of 4402 nondemented subjects, whose age ranged from 45 to 78 years. Results of the pooled meta-analysis found a significant effect of carotenoid intervention on cognitive outcomes (Hedge’s g = 0.14; 95% confidence interval: 0.08, 0.20, p < 0.0001). There was no evidence of heterogeneity among the studies (τ2 = 0.00, I2 = 0.00%, H2 = 1.00) or publication bias. Although further studies are needed, our results suggest that carotenoid interventions are associated with better cognitive performance. Thus, these dietary compounds may help to reduce the risk of cognitive impairment and dementia.
Chaos-Based Synchronized Dynamic Keys and Their Application to Image Encryption with an Improved AES Algorithm
Authors: Lin Chih-Hsueh , Hu Guo-Hsin , Chan Che-Yu , Yan Jun-Juh
Published: February 2021

This study aimed to design chaos-based synchronized dynamic keys and develop an improved chaos-based advanced encryption standard (AES) algorithm with the proposed synchronized random keys. First, based on sliding mode control (SMC) technology, a rippling control scheme was introduced to guarantee the synchronization between master–slave discrete chaotic systems. Under the synchronization, the same dynamic random chaos signals could be simultaneously obtained at the transmitter and receiver in communication systems. Then, a novel modified AES cryptosystem with dynamic random keys based on chaos synchronization was presented. In a traditional AES cryptosystem, a static key is used, and it must be exchanged in advance and confirmed to be safely kept. However, in the proposed design, by introducing the synchronization technology of chaotic systems, the static key becomes dynamic and random, and it does not need to be kept or transmitted in open channels. Consequently, the disadvantage of key storage could be eliminated and the security of encryption could be improved. Finally, the developed chaos-based AES (CAES) algorithm has been applied to construct a novel image encryption algorithm. The statistical analysis, histogram, information entropy, and correlation indexes have been calculated and analyzed through simulation experiments in order to demonstrate the capability and improvement of this presented CAES cryptosystem.
Passive Control of Base Pressure: A Review
Authors: Khan Ambareen , Rajendran Parvathy , Sidhu Junior Sarjit Singh
Published: February 2021

In the present world, passive control finds application in various areas like flow over blunt projectiles, missiles, supersonic parallel diffusers (for cruise correction), the engine of jets, static testbeds of rockets, the ports of internal combustion engines, vernier rockets, and single expansion ramp nozzle (SERN) rockets. In this review, various passive control techniques to control the base pressure and regulate the drag force are discussed. In the study, papers ranging from subsonic, sonic, and supersonic flow are discussed. Different types of passive control management techniques like cavity, ribs, dimple, static cylinder, spikes, etc., are discussed in this review article. This study found that the passive control device can control the base pressure, resulting in an enhancement in the base pressure and reducing the base drag. Also, passive control is very efficient whenever there is a favorable pressure gradient at the nozzle exit.
Leveraging the Advantages of Additive Manufacturing to Produce Advanced Hybrid Composite Structures for Marine Energy Systems
Authors: Murdy Paul , Dolson Jack , Miller David , Hughes Scott , Beach Ryan
Published: February 2021

Many marine energy systems designers and developers are beginning to implement composite materials into the load-bearing structures of their devices, but traditional mold-making costs for composite prototyping are disproportionately high and lead times can be long. Furthermore, established molding techniques for marine energy structures generally require many manufacturing steps, such as secondary bonding and tooling. This research explores the possibilities of additively manufactured internal composite molds and how they can be used to reduce costs and lead times through novel design features and processes for marine energy composite structures. In this approach, not only can the composite mold be additively manufactured but it can also serve as part of the final load-bearing structure. We developed a conceptual design and implemented it to produce a reduced-scale additive/composite tidal turbine blade section to fully demonstrate the manufacturing possibilities. The manufacturing was successful and identified several critical features that could expedite the tidal turbine blade manufacturing process, such as single-piece construction, an integrated shear web, and embedded root fasteners. The hands-on manufacturing also helped identify key areas for continued research to allow for efficient, durable, and low-cost additive/composite-manufactured structures for future marine energy systems.
Anomalous Event Recognition in Videos Based on Joint Learning of Motion and Appearance with Multiple Ranking Measures
Authors: Dubey Shikha , Boragule Abhijeet , Gwak Jeonghwan , Jeon Moongu
Published: February 2021

Given the scarcity of annotated datasets, learning the context-dependency of anomalous events as well as mitigating false alarms represent challenges in the task of anomalous activity detection. We propose a framework, Deep-network with Multiple Ranking Measures (DMRMs), which addresses context-dependency using a joint learning technique for motion and appearance features. In DMRMs, the spatial-time-dependent features are extracted from a video using a 3D residual network (ResNet), and deep motion features are extracted by integrating the motion flow maps’ information with the 3D ResNet. Afterward, the extracted features are fused for joint learning. This data fusion is then passed through a deep neural network for deep multiple instance learning (DMIL) to learn the context-dependency in a weakly-supervised manner using the proposed multiple ranking measures (MRMs). These MRMs consider multiple measures of false alarms, and the network is trained with both normal and anomalous events, thus lowering the false alarm rate. Meanwhile, in the inference phase, the network predicts each frame’s abnormality score along with the localization of moving objects using motion flow maps. A higher abnormality score indicates the presence of an anomalous event. Experimental results on two recent and challenging datasets demonstrate that our proposed framework improves the area under the curve (AUC) score by 6.5% compared to the state-of-the-art method on the UCF-Crime dataset and shows AUC of 68.5% on the ShanghaiTech dataset.
Characterizing Highly Cited Papers in Mass Cytometry through H-Classics
Authors: Di Zeo-Sánchez Daniel E. , Sánchez-Núñez Pablo , Stephens Camilla , Lucena M. Isabel
Published: February 2021

Mass cytometry (CyTOF) is a relatively novel technique for the multiparametric analysis of single-cell features with an increasing central role in cell biology, immunology, pharmacology, and biomedicine. This technique mixes the fundamentals of flow cytometry with mass spectrometry and is mainly used for in-depth studies of the immune system and diseases with a significant immune load, such as cancer, autoimmune diseases, and viral diseases like HIV or the recently emerged COVID-19, produced by the SARS-CoV-2 coronavirus. The objective of this study was to provide a useful insight into the evolution of the mass cytometry research field, revealing the knowledge structure (conceptual and social) and authors, countries, sources, documents, and organizations that have made the most significant contribution to its development. We retrieved 937 articles from the Web of Science (2010–2019), analysed 71 Highly Cited Papers (HCP) through the H-Classics methodology and computed the data by using Bibliometrix R package. HCP sources corresponded to high-impact journals, such as Nature Biotechnology and Cell, and its production was concentrated in the US, and specifically Stanford University, affiliation of the most relevant authors in the field. HCPs analysis confirmed great interest in the study of the immune system and complex data processing in the mass cytometry research field.
Collagen-Based Matrices for Osteoconduction: A Preclinical In Vivo Study
Authors: Katagiri Hiroki , El Tawil Yacine , Lang Niklaus P. , Imber Jean-Claude , Sculean Anton , Fujioka-Kobayashi Masako , Saulacic Nikola
Published: February 2021

The aim of this study was to evaluate the influence of additional hydroxyapatite (HA) in collagen-based matrices (CM) and membrane placement on bone formation in calvarial defects. Critical size defects in the calvaria of 16 New Zealand White Rabbits were randomly treated with CM or mineralized collagen-based matrices (mCM). Half of the sites were covered with a collagen membrane. Animals were euthanized after 12 weeks of healing. The samples were studied by micro-CT and histology. Newly formed lamellar bone was observed in all samples at the periphery of the defect. In the central areas, however, new bone composed of both woven and lamellar bone was embedded in the soft tissue. Samples treated with mCM showed more residual biomaterial and induced more small bony islands in the central areas of the defects than samples with CM. Nevertheless, a complete defect closure was not observed in any of the samples at 12 weeks. Membrane placement resulted in a decrease in bone density and height. Significant differences between the groups were revealed only between CM groups with and without membrane coverage for bone height in the central area of the defect. Neither mineralization of CM nor membrane placement improved the osteogenic capacity in this particular defect. Nevertheless, mineralisation influenced bone density without a membrane placement and bone volume underneath a membrane. CM may be used as a scaffold in bone regeneration procedures, without the need of a membrane coverage. Further preclinical studies are warrant to optimise the potential of mCM.
A Psychophysical Window onto the Subjective Experience of Compulsion
Authors: Schmidt Stefan , Wagner Gerd , Walter Martin , Stenner Max-Philipp
Published: February 2021

In this perspective, we follow the idea that an integration of cognitive models with sensorimotor theories of compulsion is required to understand the subjective experience of compulsive action. We argue that cognitive biases in obsessive–compulsive disorder may obscure an altered momentary, pre-reflective experience of sensorimotor control, whose detection thus requires an implicit experimental operationalization. We propose that a classic psychophysical test exists that provides this implicit operationalization, i.e., the intentional binding paradigm. We show how intentional binding can pit two ideas against each other that are fundamental to current sensorimotor theories of compulsion, i.e., the idea of excessive conscious monitoring of action, and the idea that patients with obsessive–compulsive disorder compensate for diminished conscious access to “internal states”, including states of the body, by relying on more readily observable proxies. Following these ideas, we develop concrete, testable hypotheses on how intentional binding changes under the assumption of different sensorimotor theories of compulsion. Furthermore, we demonstrate how intentional binding provides a touchstone for predictive coding accounts of obsessive–compulsive disorder. A thorough empirical test of the hypotheses developed in this perspective could help explain the puzzling, disabling phenomenon of compulsion, with implications for the normal subjective experience of human action.
Creating a Theoretical Framework to Underpin Discourse Assessment and Intervention in Aphasia
Authors: Dipper Lucy , Marshall Jane , Boyle Mary , Hersh Deborah , Botting Nicola , Cruice Madeline
Published: February 2021

Discourse (a unit of language longer than a single sentence) is fundamental to everyday communication. People with aphasia (a language impairment occurring most frequently after stroke, or other brain damage) have communication difficulties which lead to less complete, less coherent, and less complex discourse. Although there are multiple reviews of discourse assessment and an emerging evidence base for discourse intervention, there is no unified theoretical framework to underpin this research. Instead, disparate theories are recruited to explain different aspects of discourse impairment, or symptoms are reported without a hypothesis about the cause. What is needed is a theoretical framework that would clarify the specific linguistic skills that create completeness, coherence, and complexity (i.e., richness) in discourse, and illuminate both the processes involved in discourse production and the reasons for breakdown. This paper reports a review and synthesis of the theoretical literature relevant to spoken discourse in aphasia discourse, and we propose a novel theoretical framework which unites these disparate sources. This framework is currently being tested as the foundation for Linguistic Underpinnings of Narrative in Aphasia (LUNA) treatment research. In this paper, we outline the novel framework and exemplify how it might be used to guide clinical practice and research. Future collaborative research is needed to develop this framework into a processing model for spoken discourse.
Ultrasound: The Extension of Our Hands to Improve the Management of Thyroid Patients
Authors: Trimboli Pierpaolo
Published: February 2021

Ultrasonography (US) was introduced in the thyroid field in the 1980s to guide the biopsy of palpable, scintigraphically cold nodules [...]
The Role of BRCA1/2-Mutated Tumor Microenvironment in Breast Cancer
Authors: Miklikova Svetlana , Trnkova Lenka , Plava Jana , Bohac Martin , Kuniakova Marcela , Cihova Marina
Published: February 2021

Taking into account the factors of high incidence rate, prevalence and mortality, breast cancer represents a crucial social and economic burden. Most cases of breast cancer develop as a consequence of somatic mutations accumulating in mammary epithelial cells throughout lifetime and approximately 5–10% can be ascribed to monogenic predispositions. Even though the role of genetic predispositions in breast cancer is well described in the context of genetics, very little is known about the role of the microenvironment carrying the same aberrant cells impaired by the germline mutation in the breast cancer development and progression. Based on the clinical observations, carcinomas carrying mutations in hereditary tumor-suppressor genes involved in maintaining genome integrity such as BRCA1/2 have worse prognosis and aggressive behavior. One of the mechanisms clarifying the aggressive nature of BRCA-associated tumors implies alterations within the surrounding adipose tissue itself. The objective of this review is to look at the role of BRCA1/2 mutations in the context of breast tumor microenvironment and plausible mechanisms by which it contributes to the aggressive behavior of the tumor cells.
Molecular and Metabolic Mechanisms Underlying Selective 5-Aminolevulinic Acid-Induced Fluorescence in Gliomas
Authors: Traylor Jeffrey I. , Pernik Mark N. , Sternisha Alex C. , McBrayer Samuel K. , Abdullah Kalil G.
Published: February 2021

5-aminolevulinic acid (5-ALA) is a porphyrin precursor in the heme synthesis pathway. When supplied exogenously, certain cancers consume 5-ALA and convert it to the fluorogenic metabolite protoporphyrin IX (PpIX), causing tumor-specific tissue fluorescence. Preoperative administration of 5-ALA is used to aid neurosurgical resection of high-grade gliomas such as glioblastoma, allowing for increased extent of resection and progression free survival for these patients. A subset of gliomas, especially low-grade tumors, do not accumulate PpIX intracellularly or readily fluoresce upon 5-ALA administration, making gross total resection difficult to achieve in diffuse lesions. We review existing literature on 5-ALA metabolism and PpIX accumulation to explore potential mechanisms of 5-ALA-induced glioma tissue fluorescence. Targeting the heme synthesis pathway and understanding its dysregulation in malignant tissues could aid the development of adjunct therapies to increase intraoperative fluorescence after 5-ALA treatment.
Clinical Characterisation and Management of the Main Treatment-Induced Toxicities in Patients with Hepatocellular Carcinoma and Cirrhosis
Authors: Meriggi Fausto , Graffeo Massimo
Published: February 2021

The incidence of hepatocellular carcinoma (HCC) continues to increase worldwide, particularly in Western countries. In almost all cases, HCC develops in subjects with hepatic cirrhosis, often as the result of hepatitis B or C virus infection, alcohol abuse or metabolic forms secondary to non-alcoholic steatohepatitis. Patients with HCC and hepatic symptoms can therefore present symptoms that are attributable to both conditions. These patients require multidisciplinary management, calling for close interaction between the hepatologist and the oncologist. Indeed, the treatment of HCC requires, depending on the disease stage and the degree of hepatic impairment, locoregional therapies that can in turn be broken down into surgical and nonsurgical treatments and systemic treatments used in the event of progression after the administration of locoregional treatments. The past decade has seen the publication of countless papers of great interest that have radically changed the scenario of treatment for HCC. Novel therapies with biological agents and immunotherapy have come to be standard options in the approach to treatment of this cancer, obtaining very promising results where in the past chemotherapy was almost never able to have an impact on the course of the disease. However, in addition to being costly, these drugs are not devoid of adverse effects and their management cannot forgo the consideration of the underlying hepatic impairment. Patients with HCC and cirrhosis therefore require special attention, starting from the initial characterisation needed for an appropriate selection of those to be referred for treatment, as these patients are almost never fit. In this chapter, we will attempt to investigate and clarify the key points of the management of the main toxicities induced by locoregional and systemic treatments for HCC secondary to cirrhosis.
Enhanced Photocatalytic Activity of ZnO-Graphene Oxide Nanocomposite by Electron Scavenging
Authors: Ahmed Syed Nabeel , Haider Waseem
Published: February 2021

Advances in nanotechnology have opened new doors to overcome the problems related to contaminated water by introducing photocatalytic nanomaterials. These materials can effectively degrade toxic contaminants, such as dyes and other organic pollutants, into harmless by-products such as carbon dioxide and water. Consequently, these photocatalytic nanomaterials have the potential to provide low-cost and environment-friendly alternatives to conventional water and wastewater treatment techniques. In this study, a nanocomposite of zinc oxide and graphene oxide was developed and evaluated for photocatalysis. This nanocomposite was characterized by XRD, FTIR, FESEM, Diffuse Reflectance Spectroscopy (DRS), TEM and UV-Vis spectrophotometer. The photocatalytic behavior of the nanocomposite was studied through the degradation of methyl orange under ultraviolet light. It is reported that the weight ratios of zinc oxide and graphene oxide do not considerably affect the photocatalytic performance, which gives this process more compositional flexibility. Moreover, hydrogen peroxide was used as an electron scavenger to increase the time-efficiency of the process. The photodegradation rate can be significantly improved (up to 24 times) with the addition of hydrogen peroxide, which increases the number of trapped electrons and generates more oxidizing species, consequently increasing the reaction rate.
Crosstalk between the mTOR and DNA Damage Response Pathways in Fission Yeast
Authors: Alao John-Patrick , Legon Luc , Rallis Charalampos
Published: February 2021

Cells have developed response systems to constantly monitor environmental changes and accordingly adjust growth, differentiation, and cellular stress programs. The evolutionarily conserved, nutrient-responsive, mechanistic target of rapamycin signaling (mTOR) pathway coordinates basic anabolic and catabolic cellular processes such as gene transcription, protein translation, autophagy, and metabolism, and is directly implicated in cellular and organismal aging as well as age-related diseases. mTOR mediates these processes in response to a broad range of inputs such as oxygen, amino acids, hormones, and energy levels, as well as stresses, including DNA damage. Here, we briefly summarize data relating to the interplays of the mTOR pathway with DNA damage response pathways in fission yeast, a favorite model in cell biology, and how these interactions shape cell decisions, growth, and cell-cycle progression. We, especially, comment on the roles of caffeine-mediated DNA-damage override. Understanding the biology of nutrient response, DNA damage and related pharmacological treatments can lead to the design of interventions towards improved cellular and organismal fitness, health, and survival.
Simpson-Golabi-Behmel-Syndrome in Dichorionic-Diamniotic Twin Pregnancy
Authors: Reischer Theresa , Laccone Franco , Kasprian Gregor J. , Yerlikaya-Schatten Gülen
Published: February 2021

Simpson-Golabi-Behmel syndrome (SGBS) is a rare x-linked overgrowth syndrome with distinct clinical features, which is difficult to diagnose prenatally. We report the diagnosis of SGBS in dichorionic-diamniotic twin pregnancies in the first trimester by ultrasound and genetic testing. The affected fetus developed polyhydramnios and the cervical length of the mother decreased significantly. To save the unaffected twin, a selective feticide of the affected fetus was performed. Finally, the patient underwent preterm caesarean section due to premature rupture of membranes in the dead twin, and also intrauterine infection. While SGBS has been reported, this was the first case in a multiple pregnancy, with possible consequences for the healthy twin. In conclusion, SGBS is a rare condition, which should be considered in the differential diagnosis of prenatal overgrowth syndromes and associated malformation.
A Multidisciplinary Approach to Implement Personalized Breast Cancer Treatment and Care Plans
Authors: Haq Rashida , Kong Amy , Gulasingam Pauline
Published: February 2021

Implementation of survivorship care plans remain a challenge. This quality improvement initiative aims to integrate personalized treatment plans (PTP) and care plans (PCP) into the existing workflow for breast cancer (BC) patients. Methods: Phase 1 was to identify multidisciplinary team members to generate and deliver PTP and PCP. Concurrently, Phase 2 was to deliver PTP and PCP to newly diagnosed invasive BC patients at chemotherapy initiation and completion, respectively. Iterative plan, do, study, act (PDSA) cycles were applied to refine the process. The proportion of information completed for PTP and PCP generation and its delivery by the care team were measured. Patient and provider satisfaction were also assessed. Implementation Process and Results: The care transfer facilitator (CTF) was identified to complete and deliver PTP, and their data entry increased from 0% to 76%, 80%, 92% consecutively during the last 4 PDSA cycles. PTP and PCP were provided to 85% of eligible BC patients. Patients agreed that PTP helped them to actively participate in their care (88%) and communicate with the oncology care team (86%). Primary care physicians agreed that PTP and PCP had the information needed to “stay in the loop” (80%), and oncologists agreed they should be incorporated into oncology clinics (100%). Conclusions: Integrating PTP and PCP generation and delivery into existing workflow has led to an increase in uptake, sustainability and provider buy-in. With limited resources, it remains difficult to find care team members to complete the forms. A dedicated personnel or survivorship clinic is required to successfully implement PTP and PCP as the standard of care.
Diversity and Physiological Tolerance of Native and Invasive Jellyfish/Ctenophores along the Extreme Salinity Gradient of the Baltic Sea
Authors: Jaspers Cornelia , Bezio Nicholas , Hinrichsen Hans-Harald
Published: February 2021

Global change has led to manifold changes of marine ecosystems and biodiversity world-wide. While it has been shown that certain jellyfish and comb jelly species have increased regionally, it remains to be investigated if this is a general trend or localized phenomenon. Especially for the economically important Baltic Sea, which is characterized by an extreme physical environmental gradient, this question has not been addressed to date. Here we present a detailed account of the gelatinous macro-zooplankton community including their physiological tolerance towards abiotic conditions and resulting distribution ranges in the Baltic. We show that the arrival and establishment of non-indigenous species has led to a rising importance of jellyfish and comb jellies in the Baltic. This accounts for the comb jelly Mnemiopsis leidyi, which was first observed in Northern Europe in 2005, as well as for the hydromedusae Blackfordia virginica, first sighted in 2014. Both species have been shown to attain high population densities with pronounced grazing impact in other invasive regions. Given the current and anticipated changes of the physical environment of the Baltic Sea, especially ongoing warming, amplification of their impact can be expected.
Quantification of the Therapist’s Gentle Pull for Pinch Strength Testing Based on FMA and MMT: An Experimental Study with Healthy Subjects
Authors: Alsayed Abdallah , Kamil Raja , Rowe Veronica , Salim Mazatulfazura S. F. , Ramli Hafiz R. , As’arry Azizan
Published: February 2021

Static pinch strength against a therapist’s gentle pull is evaluated using the pincer grasp component of the Fugl Meyer Assessment (FMA) to assess pinch impairment after stroke. In the pincer grasp component, therapists applied a gentle pull to distinguish between a score of 1 (moderate pinch impairment) and a score of 2 (no pinch impairment). The gentle pull is described as a resistance equivalent to a manual muscle test (MMT) score 4/5. The accepted use of “gentle” as a qualitative description for the pull results is a non-standardized subjective interpretation. The goal of this paper was to determine the quantitative value of the gentle pull applied by the therapists as in their clinical practice using a pinch–pull gripping system. The FMA protocol was used to standardize the body and fingers positions of three occupational therapists who were then instructed to apply a gentle pull of 4/5 MMT using their thumb and index fingers (in a tip-to-tip pinch). The results show that the therapists exerted a mean gentle pull (4/5 MMT score) of 6.34 ± 0.98 N with high reliability and acceptable repeatability. In investigating the ability of healthy subjects to resist the gentle pull, 50 adult male volunteers were instructed to pinch the pincer object and resist a dynamic loading exerted by the pinch–pull gripping system as much as possible to the moment the pincer object slips away. The results show that all subjects were able to exert a pulling force higher than the quantitative value of the gentle pull.
Information Theoretic Metagenome Assembly Allows the Discovery of Disease Biomarkers in Human Microbiome
Authors: Nalbantoglu O. Ufuk
Published: February 2021

Quantitative metagenomics is an important field that has delivered successful microbiome biomarkers associated with host phenotypes. The current convention mainly depends on unsupervised assembly of metagenomic contigs with a possibility of leaving interesting genetic material unassembled. Additionally, biomarkers are commonly defined on the differential relative abundance of compositional or functional units. Accumulating evidence supports that microbial genetic variations are as important as the differential abundance content, implying the need for novel methods accounting for the genetic variations in metagenomics studies. We propose an information theoretic metagenome assembly algorithm, discovering genomic fragments with maximal self-information, defined by the empirical distributions of nucleotides across the phenotypes and quantified with the help of statistical tests. Our algorithm infers fragments populating the most informative genetic variants in a single contig, named supervariant fragments. Experiments on simulated metagenomes, as well as on a colorectal cancer and an atherosclerotic cardiovascular disease dataset consistently discovered sequences strongly associated with the disease phenotypes. Moreover, the discriminatory power of these putative biomarkers was mainly attributed to the genetic variations rather than relative abundance. Our results support that a focus on metagenomics methods considering microbiome population genetics might be useful in discovering disease biomarkers with a great potential of translating to molecular diagnostics and biotherapeutics applications.
From Antiblackness to Cultural Health in Higher Education
Authors: Grier-Reed Tabitha , Said Roun , Quiñones Miguel
Published: February 2021

Antiblackness has a long and storied history in higher education in the United States, and unfortunately, antiblack attitudes and practices continue in the 21st century. With implications for countering antiblackness in higher education and institutionalizing support for cultural health and wellness, we documented experiences of antiblackness in the African American Student Network (AFAM). AFAM was a weekly networking group, co-facilitated by Black faculty and graduate students, where Black undergraduates could come together and share their experiences. Participation in AFAM was associated with Black holistic wellness, and AFAM was a source of cultural health, where we conceptualized cultural health as having a sense of pride and resilience in one’s cultural background. We analyzed notes of 277 AFAM discussions from 2005–2006 to 2017–2018 using an adaptation of consensual qualitative research methods to identify four domains of antiblackness: racial trauma (n = 51), racial microaggressions (n = 34), racial rejection (n = 33), and systemic racism (n = 25). In moving from antiblackness to cultural health, we advocate for institutional resources in higher education, such as an institute for cultural health on campus, that values the cultures of Black students and students of color, and that focuses on building communities in which students can generate a wellspring of pride and resilience in their cultural backgrounds.
A GPU Scheduling Framework to Accelerate Hyper-Parameter Optimization in Deep Learning Clusters
Authors: Son Jaewon , Yoo Yonghyuk , Kim Khu-rai , Kim Youngjae , Lee Kwonyong , Park Sungyong
Published: February 2021

This paper proposes Hermes, a container-based preemptive GPU scheduling framework for accelerating hyper-parameter optimization in deep learning (DL) clusters. Hermes accelerates hyper-parameter optimization by time-sharing between DL jobs and prioritizing jobs with more promising hyper-parameter combinations. Hermes’s scheduling policy is grounded on the observation that good hyper-parameter combinations converge quickly in the early phases of training. By giving higher priority to fast-converging containers, Hermes’s GPU preemption mechanism can accelerate training. This enables users to find optimal hyper-parameters faster without losing the progress of a container. We have implemented Hermes over Kubernetes and compared its performance against existing scheduling frameworks. Experiments show that Hermes reduces the time for hyper-parameter optimization up to 4.04 times against previously proposed scheduling policies such as FIFO, round-robin (RR), and SLAQ, with minimal time-sharing overhead.
Evaluating the Potential Benefit of Using Nowcasting Systems to Improve the Yield of Parabolic Trough Power Plants with Single-Phase HTF
Authors: Noureldin Kareem , Hirsch Tobias , Nouri Bijan , Yasser Zeyad , Pitz-Paal Robert
Published: February 2021

Solar field developers include innovative solutions to optimize the energy production of their plants. Simulation tools play a significant role in the design and testing phases as they provide estimations of this yield in different conditions. Transient processes, like passing clouds and solar field start-up, are specifically challenging to optimize and estimate using such simulation tools. Solar fields are subject to high degree of both temporal and spatial variability in the energy input and a detailed estimation can be achieved by simulating subsystems within acceptable time and computational power. Hence, such simulation tools cannot be utilized for tests under realistic operation conditions. The Virtual Solar Field is a computationally efficient simulation tool that allows a detailed transient simulation of parabolic trough solar fields based on single-phase fluids. Using this tool, developers could reproduce a transient test case with exactly the same disturbances to provide fair comparisons between different configurations. In this paper, an evaluation process based on numerical simulations using the Virtual Solar Field is presented. The economic benefit of novel innovative control concepts can be assessed according to the presented scheme. This is demonstrated by evaluating the potential benefit of availability of spatial DNI nowcasts on the control of parabolic trough solar fields. Results show that nowcasting can increase the economic revenue of commercial power plants by up to 2.5% per day. This proves the feasibility of installing such systems.
Purification of Methyl Acetate/Water Mixtures from Chemical Interesterification of Vegetable Oils by Pervaporation
Authors: Casas Abraham , Pérez Ángel , Ramos María Jesús
Published: February 2021

Biodiesel production through chemical interesterification of triglycerides requires an excess of methyl acetate that must be recovered once the reaction is finished and the catalyst is neutralized. The present study concerns with the purification of methyl acetate by pervaporation. PERVAP 2201 was chosen as pervaporation membrane due to its high hydrophilic character that makes it suitable for the elimination of water in methyl acetate. Runs were started from concentrations in the feed of 2–8 wt.% of water and working temperatures close to the boiling point of methyl acetate (50, 60, and 70 °C), to get the main design parameters, i.e., permeate flux and selectivity. High temperature favored the permeate flux without compromising the selectivity. However, the flux declines significantly when water contained in the feed is below 2 wt.%. This implies that pervaporation should be used, only to decrease the water content to a value lower than in the azeotrope (2.3% by weight). A solution-diffusion model relating the flux of the permeating compound with the activity of the compound in the feed and the operating temperature has been proposed. The model obtained can be used in the design of the pervaporation stage, thus allowing to know the permeate flux for the different operating conditions.
Improving the Anaerobic Digestion of Swine Manure through an Optimized Ammonia Treatment: Process Performance, Digestate and Techno-Economic Aspects
Authors: Lymperatou Anna , Rasmussen Niels B. , Gavala Hariklia N. , Skiadas Ioannis V.
Published: February 2021

Swine manure mono-digestion results in relatively low methane productivity due to the low degradation rate of its solid fraction (manure fibers), and due to the high ammonia and water content. The aqueous ammonia soaking (AAS) pretreatment of manure fibers has been proposed for overcoming these limitations. In this study, continuous anaerobic digestion (AD) of manure mixed with optimally AAS-treated manure fibers was compared to the AD of manure mixed with untreated manure fibers. Due to lab-scale pumping restrictions, the ratio of AAS-optimally treated manure fibers to manure was only 1/3 on a total solids (TS) basis. However, the biogas productivity and methane yield were improved by 17% and 38%, respectively, also confirming the predictions from a simplified 1st order hydrolysis model based on batch experiments. Furthermore, an improved reduction efficiency of major organic components was observed for the digester processing AAS-treated manure fibers compared to the non-treated one (e.g., 42% increased reduction for cellulose fraction). A preliminary techno-economic analysis of the proposed process showed that mixing raw manure with AAS manure fibers in large-scale digesters could result in a 72% increase of revenue compared to the AD of manure mixed with untreated fibers and 135% increase compared to that of solely manure.
Evaluating Forest Visitors’ Place Attachment, Recreational Activities, and Travel Intentions under Different Climate Scenarios
Authors: Liu Wan-Yu , Yu Hung-Wen , Hsieh Chi-Ming
Published: February 2021

Climate change leads to a variety of extreme weather conditions such as heat waves, unusual cold weather, and heavy rain, which always ends up with serious disasters. It could have a tremendous impact on a lot of industries in the world. The tourist industry, that plays a vital role in the global economy, has faced serious impacts from climate change in many tourist attractions, e.g., national parks, mountain areas, and beaches. The travel behavior of visitors has been changed under various climate change conditions. To understand the influence degrees of tourists on climate change factors, this study aimed to analyze whether there is difference in the influence of climate change on socio-demographic background, travel activities, travel intention, and the revisit intention attitude of tourists with different degrees of place attachment to Wuling National Forest Recreation Area. This study further investigated whether the climate change adaptation strategies offered by the park manager would have a positive influence on travel intention of tourists. The results of this study showed that except for heat waves, events related to climate change such as stronger typhoon, heavy rain, unusual cold weather condition, mud sliding, forest fire, and the appearance of mosquitos would have a negative influence on travel intention, especially for the tourists with a low degree of place attachment. In addition, if the park manager offers strategies to adapt to climate change conditions, these strategies would have a positive influence on travel intention.