bluuebunny/crossref_metadata_2025 huggingface dataset:
Antimicrobial Resistance in Equine Reproduction
Authors: Malaluang Pongpreecha , Wilén Elin , Lindahl Johanna , Hansson Ingrid , Morrell Jane M.
Published: October 2021

Bacteria develop resistance to antibiotics following low-level “background” exposure to antimicrobial agents as well as from exposure at therapeutic levels during treatment for bacterial infections. In this review, we look specifically at antimicrobial resistance (AMR) in the equine reproductive tract and its possible origin, focusing particularly on antibiotics in semen extenders used in preparing semen doses for artificial insemination. Our review of the literature indicated that AMR in the equine uterus and vagina were reported worldwide in the last 20 years, in locations as diverse as Europe, India, and the United States. Bacteria colonizing the mucosa of the reproductive tract are transferred to semen during collection; further contamination of the semen may occur during processing, despite strict attention to hygiene at critical control points. These bacteria compete with spermatozoa for nutrients in the semen extender, producing metabolic byproducts and toxins that have a detrimental effect on sperm quality. Potential pathogens such as Klebsiella pneumoniae, Escherichia coli, and Pseudomonas aeruginosa may occasionally cause fertility issues in inseminated mares. Antibiotics are added during semen processing, according to legislation, to impede the growth of these microorganisms but may have a detrimental effect on sperm quality, depending on the antimicrobial agent and concentration used. However, this addition of antibiotics is counter to current recommendations on the prudent use of antibiotics, which recommend that antibiotics should be used only for therapeutic purposes and after establishing bacterial sensitivity. There is some evidence of resistance among bacteria found in semen samples. Potential alternatives to the addition of antibiotics are considered, especially physical removal separation of spermatozoa from bacteria. Suggestions for further research with colloid centrifugation are provided.
Melatonin Reduces Oxidative Stress in the Right Ventricle of Newborn Sheep Gestated under Chronic Hypoxia
Authors: Gonzaléz-Candia Alejandro , Arias Pamela V. , Aguilar Simón A. , Figueroa Esteban G. , Reyes Roberto V. , Ebensperger Germán , Llanos Aníbal J. , Herrera Emilio A.
Published: October 2021

Pulmonary arterial hypertension of newborns (PAHN) constitutes a critical condition involving both severe cardiac remodeling and right ventricle dysfunction. One main cause of this condition is perinatal hypoxia and oxidative stress. Thus, it is a public health concern for populations living above 2500 m and in cases of intrauterine chronic hypoxia in lowlands. Still, pulmonary and cardiac impairments in PAHN lack effective treatments. Previously we have shown the beneficial effects of neonatal melatonin treatment on pulmonary circulation. However, the cardiac effects of this treatment are unknown. In this study, we assessed whether melatonin improves cardiac function and modulates right ventricle (RV) oxidative stress. Ten lambs were gestated, born, and raised at 3600 m. Lambs were divided in two groups. One received daily vehicle as control, and another received daily melatonin (1 mg·kg−1·d−1) for 21 days. Daily cardiovascular measurements were recorded and, at 29 days old, cardiac tissue was collected. Melatonin decreased pulmonary arterial pressure at the end of the experimental period. In addition, melatonin enhanced manganese superoxide dismutase and catalase (CAT) expression, while increasing CAT activity in RV. This was associated with a decrease in superoxide anion generation at the mitochondria and NADPH oxidases in RV. Finally, these effects were associated with a marked decrease of oxidative stress markers in RV. These findings support the cardioprotective effects of an oral administration of melatonin in newborns that suffer from developmental chronic hypoxia.
Predicting High-Risk Groups for COVID-19 Anxiety Using AdaBoost and Nomogram: Findings from Nationwide Survey in South Korea
Authors: Byeon Haewon
Published: October 2021

People living in local communities have become more worried about infection due to the extended pandemic situation and the global resurgence of COVID-19. In this study, the author (1) selected features to be included in the nomogram using AdaBoost, which had an advantage in increasing the classification accuracy of single learners and (2) developed a nomogram for predicting high-risk groups of coronavirus anxiety while considering both prediction performance and interpretability based on this. Among 210,606 adults (95,287 males and 115,319 females) in South Korea, 39,768 people (18.9%) experienced anxiety due to COVID-19. The AdaBoost model confirmed that education level, awareness of neighbors/colleagues’ COVID-19 response, age, gender, and subjective stress were five key variables with high weight in predicting anxiety induced by COVID-19 for adults living in South Korean communities. The developed logistic regression nomogram predicted that the risk of anxiety due to COVID-19 would be 63% for a female older adult who felt a lot of subjective stress, did not attend a middle school, was 70.6 years old, and thought that neighbors and colleagues responded to COVID-19 appropriately (classification accuracy = 0.812, precision = 0.761, recall = 0.812, AUC = 0.688, and F-1 score = 0.740). Prospective or retrospective cohort studies are required to causally identify the characteristics of anxiety disorders targeting high-risk COVID-19 anxiety groups identified in this study.
A Local Adaptive Mesh Refinement for JFO Cavitation Model on Cartesian Meshes
Authors: Xu Wanjun , Li Kang , Geng Zhengyang , Zhang Mingjie , Yang Jiangang
Published: October 2021

Nonuniform mesh is beneficial to reduce computational cost and improve the resolution of the interest area. In the paper, a cell-based adaptive mesh refinement (AMR) method was developed for bearing cavitation simulation. The bearing mesh can be optimized by local refinement and coarsening, allowing for a reasonable solution with special purpose. The AMR algorithm was constructed based on a quadtree data structure with a Z-order filling curve managing cells. The hybrids of interpolation schemes on hanging nodes were applied. A cell matching method was used to handle periodic boundary conditions. The difference schemes at the nonuniform mesh for the universal Reynolds equation were derived. Ausas’ cavitation algorithm was integrated into the AMR algorithm. The Richardson extrapolation method was employed as an a posteriori error estimation to guide the areas where they need to be refined. The cases of a journal bearing and a thrust bearing were studied. The results showed that the AMR method provided nearly the same accuracy results compared with the uniform mesh, while the number of mesh was reduced to 50–60% of the number of the uniform mesh. The computational efficiency was effectively improved. The AMR method is suggested to be a potential tool for bearing cavitation simulation.
A Study of an EOQ Model of Growing Items with Parabolic Dense Fuzzy Lock Demand Rate
Authors: Maity Suman , De Sujit , Pal Madhumangal , Mondal Sankar
Published: October 2021

In this article, the parabolic dense fuzzy set is defined, and its basic arithmetic operations are studied with graphical illustration. The lock set concept is incorporated in a parabolic dense fuzzy set. Then, it is applied to the problems of fishery culture via the modeling of an economic order quantity model. Here, the fingerlings are fed to reach the ideal size to fulfill the customer’s demand. The growth rate of the fingerlings is assumed as a linear function. After the sales of all fish, the pond is cleaned properly for a new cycle. Here, the model is solved in a crisp sense first. Then, we fuzzify the model considering the demand rate as a parabolic dense lock fuzzy number and obtain the result in a fuzzy environment. The main aim of our study was to find the quantity of the ordering items such that the total inventory cost gets a minimum value. Lastly, sensitivity analysis and graphical illustrations were added for better justification of our model.
Carbon Dioxide and Methane Emissions during the Composting and Vermicomposting of Sewage Sludge under the Effect of Different Proportions of Straw Pellets
Authors: Dume Bayu , Hanc Ales , Svehla Pavel , Míchal Pavel , Chane Abraham Demelash , Nigussie Abebe
Published: October 2021

Owing to rapid population growth, sewage sludge poses a serious environmental threat across the world. Composting and vermicomposting are biological technologies commonly used to stabilize sewage sludge. The objective of this study was to assess the carbon dioxide (CO2) and methane (CH4) emissions from sewage sludge composting and vermicomposting under the influence of different proportions of straw pellets. Four treatments were designed, by mixing the initial sewage sludge with varying ratio of pelletized wheat straw (0, 25%, 50%, and 75% (w/w)). The experiment was conducted for 60 days, and Eisenia andrei was used for vermicomposting. The results revealed that the mixing ratio influenced CO2 (F = 36.1, p = 0.000) and CH4 (F= 73.9, p = 0.000) emissions during composting and CO2 (F= 13.8, p = 0.000) and CH4 (F= 4.5, p= 0.004) vermicomposting. Vermicomposting significantly reduced CH4 emissions by 18–38%, while increasing CO2 emissions by 64–89%. The mixing agent (pelletized wheat straw) decreased CO2 emission by 60–70% and CH4 emission by 30–80% compared to control (0%). The mass balance indicated that 5.5–10.4% of carbon was loss during composting, while methane release accounted for 0.34–1.69%, and CO2 release accounted for 2.3–8.65%. However, vermicomposting lost 8.98–13.7% of its carbon, with a methane release of 0.1–0.6% and CO2 release of 5.0–11.6% of carbon. The carbon loss was 3.3–3.5% more under vermicomposting than composting. This study demonstrated that depending on the target gas to be reduced, composting and vermicomposting, as well as a mixing agent (pelletized wheat straw), could be an option for reducing greenhouse gas emissions (i.e. CH4, CO2).
Abundance of NO3 Derived Organo-Nitrates and Their Importance in the Atmosphere
Authors: Foulds Amy , Khan M. Anwar H. , Bannan Thomas J. , Percival Carl J. , Lowenberg Mark H. , Shallcross Dudley E.
Published: October 2021

The chemistry of the nitrate radical and its contribution to organo-nitrate formation in the troposphere has been investigated using a mesoscale 3-D chemistry and transport model, WRF-Chem-CRI. The model-measurement comparisons of NO2, ozone and night-time N2O5 mixing ratios show good agreement supporting the model’s ability to represent nitrate (NO3) chemistry reasonably. Thirty-nine organo-nitrates in the model are formed exclusively either from the reaction of RO2 with NO or by the reaction of NO3 with alkenes. Temporal analysis highlighted a significant contribution of NO3-derived organo-nitrates, even during daylight hours. Night-time NO3-derived organo-nitrates were found to be 3-fold higher than that in the daytime. The reactivity of daytime NO3 could be more competitive than previously thought, with losses due to reaction with VOCs (and subsequent organo-nitrate formation) likely to be just as important as photolysis. This has highlighted the significance of NO3 in daytime organo-nitrate formation, with potential implications for air quality, climate and human health. Estimated atmospheric lifetimes of organo-nitrates showed that the organo-nitrates act as NOx reservoirs, with particularly short-lived species impacting on air quality as contributors to downwind ozone formation.
Applications and Limitations of Quantifying Speciated and Source-Apportioned VOCs with Metal Oxide Sensors
Authors: Okorn Kristen , Hannigan Michael
Published: October 2021

While low-cost air quality sensor quantification has improved tremendously in recent years, speciated hydrocarbons have received little attention beyond total lumped volatile organic compounds (VOCs) or total non-methane hydrocarbons (TNMHCs). In this work, we attempt to use two broad response metal oxide VOC sensors to quantify a host of speciated hydrocarbons as well as smaller groups of hydrocarbons thought to be emanating from the same source or sources. For sensors deployed near oil and gas facilities, we utilize artificial neural networks (ANNs) to calibrate our low-cost sensor signals to regulatory-grade measurements of benzene, toluene, and formaldehyde. We also use positive matrix factorization (PMF) to group these hydrocarbons along with others by source, such as wet and dry components of oil and gas operations. The two locations studied here had different sets of reference hydrocarbon species measurements available, helping us determine which specific hydrocarbons and VOC mixtures are best suited for this approach. Calibration fits on the upper end reach above R2 values of 0.6 despite the parts per billion (ppb) concentration ranges of each, which are magnitudes below the manufacturer’s prescribed detection limits for the sensors. The sensors generally captured the baseline trends in the data, but failed to quantitatively estimate larger spikes that occurred intermittently. While compounds with high variability were not suited for this method, its success with several of the compounds studied represents a crucial first step in low-cost VOC speciation. This work has important implications in improving our understanding of the links between health and environment, as different hydrocarbons will have varied consequences in the human body and atmosphere.
Study of Radioactivity in Bajaur Norite Exposed in the Himalayan Tectonic Zone of Northern Pakistan
Authors: Younis Hannan , Ahmad Farooq , Shehzadi Ramoona , Asghar Ishrat , Ahmad Tanveer , Ajaz Muhammad , Waqas Muhammad , Mehboob Khurram , Qureshi Aziz Ahmad , Haj Ismail Abd Al Karim
Published: October 2021

Radioactivity in Granites of Pakistan systematically increases from south to north. The Ambella Granite found at the northern edge of Pakistan is highly radioactive. Radioactivity measurements made on, so called, Bajaur Granite, located in northern Pakistan, have been found to be lowest among all the granitic rock of the area. In order to find out the exact nature of Bajaur rocks, mineralogical studies were carried on rock chips and powdered samples. The Bajaur Norite contains plagioclase feldspar more than 45% as the chief constituent. Orthopyroxene and clinopyroxene are 27% and 18%. Quartz, biotite, and some opaque minerals are also found in accessory amounts. Bajaur Granite is in fact not a granite but Norite, which is rich in Na-Ca plagioclase series of feldspars. The plagioclase feldspar rich in Na-Ca are low in radioactivity. Moreover, the average gamma activities of 226Ra, 232Th, and 40K (4.98 ± 0.13 Bqkg −1, 4.03 ± 0.31 Bqkg −1, 204.40 ± 4.72 Bqkg −1 and a total of all three radionuclides are 214.00 ± 5.39 Bqkg −1) for Bajaur Norites are found too be much less than the average of the world’s Granites. Indoor and outdoor hazard indices of Bajaur Norite are much below building materials used throughout the world and largely beneath their criterion restrictions. As per radiations’ hazards are concerned, the Bajaur Norite as a building stone may be considered as the safest material available in the area that does not pose any radiological hazard.
Skin Manifestations after Ionizing Radiation Exposure: A Systematic Review
Authors: Bennardo Luigi , Passante Maria , Cameli Norma , Cristaudo Antonio , Patruno Cataldo , Nisticò Steven Paul , Silvestri Martina
Published: October 2021

Morphological and functional skin alterations secondary to the action of ionizing radiation are well documented. In addition to its application in the medical field, ionizing radiation represents a public health problem for diagnostic and therapeutic purposes due to the potential risk of exposure to unexpected events, such as nuclear accidents or malicious acts. With regard to the use of ionizing radiations in the medical field, today, they constitute a fundamental therapeutic method for various neoplastic pathologies. Therefore, the onset of adverse skin events induced by radiation represents a widespread and not negligible problem, affecting 95% of patients undergoing radiotherapy. A systematic literature search was performed from July 2021 up to August 2021 using PubMed, Embase, and Cochrane databases. Articles were screened by title, abstract and full text as needed. A manual search among the references of the included papers was also performed. This systematic review describes the various skin reactions that can arise following exposure to ionizing radiation and which significantly impact the quality of life, especially in cancer patients.
“O Sister, Where Art Thou?”—A Review on Rescue of Imperiled Individuals in Ants
Authors: Miler Krzysztof , Turza Filip
Published: October 2021

Altruism is defined as an action that decreases the lifetime direct fitness of an actor and benefits one or more recipients. This phenomenon, which is generally difficult to understand and explain, requires special research attention. The subject of this review, rescue, is a type of altruistic behavior in which the actor puts itself at risk to save another individual, the recipient, that is in danger. The highest numbers of published empirical works have been devoted to rescue behavior in ants and they have enormous potential for further study. We review studies devoted to the subject and group them into four main areas of research on ant rescue actions: (1) variation in rescue behavior activity on a between-individual scale, (2) factors contributing to the evolution of rescue behavior on a between-species scale, (3) rescue behavior releaser signals and (4) rescue behavior benefits and costs. We highlight the progress in research on rescue behavior in ants, indicate that this behavior is probably much more common than previously thought yet thus far demonstrated in only a few species, and uncover research gaps and open questions that remain unexplored. We additionally point out some gaps in knowledge that become evident when research devoted to rescue behavior in rats, the second most studied group of animals in this context, is briefly overviewed. We hope to help navigate among studies on rescue behavior and provide the most up-to-date summary of the relevant literature. Moreover, we hope to encourage and facilitate researchers in behavioral ecology and other subdisciplines to further experimentally analyze rescue behavior, not only in ants but also in other taxa.
Auto-Regulation of Transcription and Translation: Oscillations, Excitability and Intermittency
Authors: Murray Philip J. , Ocana Eleonore , Meijer Hedda A. , Dale Jacqueline Kim
Published: October 2021

Several members of the Hes/Her family, conserved targets of the Notch signalling pathway, encode transcriptional repressors that dimerise, bind DNA and self-repress. Such autoinhibition of transcription can yield homeostasis and, in the presence of delays that account for processes such as transcription, splicing and transport, oscillations. Whilst previous models of autoinhibition of transcription have tended to treat processes such as translation as being unregulated (and hence linear), here we develop and explore a mathematical model that considers autoinhibition of transcription together with nonlinear regulation of translation. It is demonstrated that such a model can yield, in the absence of delays, nonlinear dynamical behaviours such as excitability, homeostasis, oscillations and intermittency. These results indicate that regulation of translation as well as transcription allows for a much richer range of behaviours than is possible with autoregulation of transcription alone. A number of experiments are suggested that would that allow for the signature of autoregulation of translation as well as transcription to be experimentally detected in a Notch signalling system.
Dissecting Abdominal Aortic Aneurysm Is Aggravated by Genetic Inactivation of LIGHT (TNFSF14)
Authors: Herrero-Cervera Andrea , Espinós-Estévez Carla , Martín-Vañó Susana , Taberner-Cortés Alida , Aguilar-Ballester María , Vinué Ángela , Piqueras Laura , Martínez-Hervás Sergio , González-Navarro Herminia
Published: October 2021

Abdominal aortic aneurysm (AAA), is a complex disorder characterized by vascular vessel wall remodeling. LIGHT (TNFSF14) is a proinflammatory cytokine associated with vascular disease. In the present study, the impact of genetic inactivation of Light was investigated in dissecting AAA induced by angiotensin II (AngII) in the Apolipoprotein E-deficient (Apoe−/−) mice. Studies in aortic human (ah) vascular smooth muscle cells (VSMC) to study potential translation to human pathology were also performed. AngII-treated Apoe−/−Light−/− mice displayed increased abdominal aorta maximum diameter and AAA severity compared with Apoe−/− mice. Notably, reduced smooth muscle α-actin+ area and Acta2 and Col1a1 gene expression were observed in AAA from Apoe−/−Light−/− mice, suggesting a loss of VSMC contractile phenotype compared with controls. Decreased Opn and augmented Sox9 expression, which are associated with detrimental and non-contractile osteochondrogenic VSMC phenotypes, were also seen in AngII-treated Apoe−/−Light−/− mouse AAA. Consistent with a role of LIGHT preserving VSMC contractile characteristics, LIGHT-treatment of ahVSMCs diminished the expression of SOX9 and of the pluripotency marker CKIT. These effects were partly mediated through lymphotoxin β receptor (LTβR) as the silencing of its gene ablated LIGHT effects on ahVSMCs. These studies suggest a protective role of LIGHT through mechanisms that prevent VSMC trans-differentiation in an LTβR-dependent manner.
Epilepsy and Ecstatic Experiences: The Role of the Insula
Authors: Picard Fabienne , Bossaerts Peter , Bartolomei Fabrice
Published: October 2021

Ecstatic epilepsy is a rare form of focal epilepsy in which the aura (beginning of the seizures) consists of a blissful state of mental clarity/feeling of certainty. Such a state has also been described as a “religious” or mystical experience. While this form of epilepsy has long been recognized as a temporal lobe epilepsy, we have accumulated evidence converging toward the location of the symptomatogenic zone in the dorsal anterior insula during the 10 last years. The neurocognitive hypothesis for the genesis of a mental clarity is the suppression of the interoceptive prediction errors and of the unexpected surprise associated with any incoming internal or external signal, usually processed by the dorsal anterior insula. This mimics a perfect prediction of the world and induces a feeling of certainty. The ecstatic epilepsy is thus an amazing model for the role of anterior insula in uncertainty and surprise.
A Study on the Causes and Effects of Stressful Situations in Tourism for Japanese People
Authors: Nghiêm-Phú Bình , Shibuya Kazuki
Published: October 2021

(1) Numerous studies have been undertaken to investigate the perceived impacts of tourism, particularly from the perspective of local residents. Only a handful have dealt with the coping strategies of this group. In addition, they have largely neglected the successes or failures of the coping strategies and the related consequences. In order to address these gaps, this study aims to investigate the psychological impacts of tourism, focusing on the causes and effects of the negative feelings felt by local residents. (2) Methods: Several qualitative methods, including web archive research, netnography, user-generated content analysis, literature review, and ethnography were employed to gather the necessary data. Japan was selected as the context of this study. (3) Results: This study identified a set of negative feelings and a group of four coping strategies. It also found that the causes of the negative feelings, the bad impacts of tourism, were similar to those in other countries. In addition, the study verified that the effects of the coping strategies were only situational and temporal. (4) Conclusions: Tourism is not stress-free. In order for tourism to sustain, the causes and consequences of its negative impacts must be properly addressed.
Cure the Incurable? Recent Breakthroughs in Immune Checkpoint Blockade for Hepatocellular Carcinoma
Authors: Chu Pei-Yi , Chan Shih-Hsuan
Published: October 2021

HCC usually arises from a chronic inflammation background, driven by several factors including fatty liver, HBV/HCV viral infection and metabolic syndrome. Systemic treatment for advanced HCC remains disappointing due to its strong resistance to chemotherapy and even to tyrosine kinase inhibitors (TKIs). Recently, the use of ICI therapy has revolutionized the systemic treatment of advanced HCC. For the first time, clinical trials testing ICIs, anti-CTLA-4 and anti-PD1/PDL1 reported a survival benefit in patients with sorafenib resistance. However, it took four more years to find the right combination regimen to use ICI in combination with the anti-angiogenic agent bevacizumab to substantially prolong overall survival (OS) of patients with advanced HCC after sorafenib. This review provides a comprehensive history of ICI therapy in HCC, up-to-date information on the latest ICI clinical trials, and discusses the recent development of novel ICIs that would potentially lead to a new checkpoint blockade therapy for advanced HCC.
Gram-Negative Bacterial Lipopolysaccharide Promotes Tumor Cell Proliferation in Breast Implant-Associated Anaplastic Large-Cell Lymphoma
Authors: Mempin Maria , Hu Honghua , Vickery Karen , Kadin Marshall E. , Prince H. Miles , Kouttab Nicola , Morgan John W. , Adams William P. , Deva Anand K.
Published: October 2021

Breast implant-associated anaplastic large-cell lymphoma (BIA-ALCL) is a distinct malignancy associated with textured breast implants. We investigated whether bacteria could trigger the activation and multiplication of BIA-ALCL cells in vitro. BIA-ALCL patient-derived BIA-ALCL tumor cells, BIA-ALCL cell lines, cutaneous ALCL cell lines, an immortal T-cell line (MT-4), and peripheral blood mononuclear cells (PBMC) from BIA-ALCL, capsular contracture, and primary augmentation patients were studied. Cells were subjected to various mitogenic stimulation assays including plant phytohemagglutinin (PHA), Gram-negative bacterial lipopolysaccharide (LPS), Staphylococcal superantigens enterotoxin A (SEA), toxic shock syndrome toxin-1 (TSST-1), or sterilized implant shells. Patient-derived BIA-ALCL tumor cells and BIA-ALCL cell lines showed a unique response to LPS stimulation. This response was dampened significantly in the presence of a Toll-like receptor 4 (TLR4) inhibitor peptide. In contrast, cutaneous ALCL cells, MT-4, and PBMC cells from all patients responded significantly more to PHA, SEA, and TSST-1 than to LPS. Breast implant shells of all surface grades alone did not produce a proliferative response of BIA-ALCL cells, indicating the breast implant does not act as a pro-inflammatory stimulant. These findings indicate a possible novel pathway for LPS to promote BIA-ALCL cell proliferation via a TLR4 receptor-mediated bacterial transformation of T-cells into malignancy.
Compound Endoscopic Morphological Features for Identifying Non-Pedunculated Lesions ≥20 mm with Intramucosal Neoplasia
Authors: da Costa-Seixas João Pedro , López-Cerón María , Arnau Anna , Rosiñol Òria , Cuatrecasas Miriam , Herreros-de-Tejada Alberto , Ferrández Ángel , Serra-Burriel Miquel , Nogales Óscar , de Castro Luisa , López-Vicente Jorge , Vega Pablo , Álvarez-González Marco A. , González-Santiago Jesús M. , Hernández-Conde Marta , Diez-Redondo Pilar , Rivero-Sánchez Liseth , Gimeno-García Antonio Z. , Burgos Aurora , García-Alonso Francisco Javier , Bustamante-Balén Marco , Martínez-Bauer Eva , Peñas Beatriz , Rodríguez-Alcalde Daniel , Pellisé Maria , Puig Ignasi
Published: October 2021

Background: The major limitation of piecemeal endoscopic mucosal resection (EMR) is the inaccurate histological assessment of the resected specimen, especially in cases of submucosal invasion. Objective: To classify non-pedunculated lesions ≥20 mm based on endoscopic morphological features, in order to identify those that present intramucosal neoplasia (includes low-grade neoplasia and high-grade neoplasia) and are suitable for piecemeal EMR. Design: A post-hoc analysis from an observational prospective multicentre study conducted by 58 endoscopists at 17 academic and community hospitals was performed. Unbiased conditional inference trees (CTREE) were fitted to analyse the association between intramucosal neoplasia and the lesions’ endoscopic characteristics. Result: 542 lesions from 517 patients were included in the analysis. Intramucosal neoplasia was present in 484 of 542 (89.3%) lesions. A conditional inference tree including all lesions’ characteristics assessed with white light imaging and narrow-band imaging (NBI) found that ulceration, pseudodepressed type and sessile morphology changed the accuracy for predicting intramucosal neoplasia. In ulcerated lesions, the probability of intramucosal neoplasia was 25% (95%CI: 8.3–52.6%; p < 0.001). In non-ulcerated lesions, its probability in lateral spreading lesions (LST) non-granular (NG) pseudodepressed-type lesions rose to 64.0% (95%CI: 42.6–81.3%; p < 0.001). Sessile morphology also raised the probability of intramucosal neoplasia to 86.3% (95%CI: 80.2–90.7%; p < 0.001). In the remaining 319 (58.9%) non-ulcerated lesions that were of the LST-granular (G) homogeneous type, LST-G nodular-mixed type, and LST-NG flat elevated morphology, the probability of intramucosal neoplasia was 96.2% (95%CI: 93.5–97.8%; p < 0.001). Conclusion: Non-ulcerated LST-G type and LST-NG flat elevated lesions are the most common non-pedunculated lesions ≥20 mm and are associated with a high probability of intramucosal neoplasia. This means that they are good candidates for piecemeal EMR. In the remaining lesions, further diagnostic techniques like magnification or diagnostic +/− therapeutic endoscopic submucosal dissection should be considered.
New Therapeutic Strategy for Overcoming Multidrug Resistance in Cancer Cells with Pyrazolo[3,4-d]pyrimidine Tyrosine Kinase Inhibitors
Authors: Podolski-Renić Ana , Dinić Jelena , Stanković Tijana , Tsakovska Ivanka , Pajeva Ilza , Tuccinardi Tiziano , Botta Lorenzo , Schenone Silvia , Pešić Milica
Published: October 2021

Tyrosine kinase inhibitors (TKIs) often interact with the multidrug resistant (MDR) phenotype of cancer cells. In some cases, TKIs increase the susceptibility of MDR cancer cells to chemotherapy. As the overexpression of membrane transporter P-glycoprotein (P-gp) is the most common alteration in MDR cancer cells, we investigated the effects of TKI pyrazolo[3,4-d]pyrimidines on P-gp inhibition in two cellular models comprising sensitive and corresponding MDR cancer cells (human non-small cell lung carcinoma and colorectal adenocarcinoma). Tested TKIs showed collateral sensitivity by inducing stronger inhibition of MDR cancer cell line viability. Moreover, TKIs directly interacted with P-gp and inhibited its ATPase activity. Their potential P-gp binding site was proposed by molecular docking simulations. TKIs reversed resistance to doxorubicin and paclitaxel in a concentration-dependent manner. The expression studies excluded the indirect effect of TKIs on P-gp through regulation of its expression. A kinetics study showed that TKIs decreased P-gp activity and this effect was sustained for seven days in both MDR models. Therefore, pyrazolo[3,4-d]pyrimidines with potential for reversing P-gp-mediated MDR even in prolonged treatments can be considered a new therapeutic strategy for overcoming cancer MDR.
Performance Study of Zn-Co-Ni/AC Catalyst in Acetylene Acetylation
Authors: Xu Zhuang , He Peijie , Chen Yuhao , Zhu Mingyuan , Wang Xugen , Dai Bin
Published: October 2021

Zinc acetate (Zn(OAc)2) loaded on activated carbon (AC) is the most commonly used catalyst for the industrial synthesis of vinyl acetate (VAc) using the acetylene method. The aim of this study is to optimize the Zn(OAc)2/AC catalyst by adding co-catalysts to improve its activity and stability. Ternary catalysts were synthesized by adding Co and Ni to the Zn(OAc)2/AC catalyst (Zn-Co-Ni/AC). Due to the strong synergistic effect among promoter Co, Ni, and the active component of Zn(OAc)2, the resulting catalyst is capable to absorb more acetic acid and less acetylene. The stability and activity of Zn-Co-Ni/AC catalyst have been improved through electron transfer to alter the electron cloud density around the Zn element. Under the same reaction conditions, the activity of Zn-Co-Ni/AC catalyst was enhanced by 83% compared to that of Zn(OAc)2/AC, and the activity was still as high as 30.1% after 120 h of testing.
Pd-HPW/SiO2 Bi-Functional Catalyst: Sonochemical Synthesis, Characterization, and Effect on Octahydroquinazolinone Synthesis
Authors: Bakht Md. Afroz , Alotaibi Mshari. , Alharthi Abdulrahman I. , Din Israf Ud , Ali Abuzer , Ali Amena , Ahsan Mohamed Jawed
Published: October 2021

A Palladium-doped silica-supported heteropoly acid (HPW) (1%Pd-HPW/SiO2) bi-functional catalyst was produced using ultrasonic and conventional procedures. Both forms of catalyst were characterized with distinct analytical approaches in order to access the advantages of each one. The presence of the required functional groups in the catalyst was confirmed using FT-IR. The crystallinity of ultrasonically generated 1%Pd-HPW/SiO2 was confirmed with XRD. The existence of necessary elements in the catalyst was also suggested by XPS and EDX data. BET was used to calculate the surface area of the ultrasonically synthesized catalyst (395 m2 g−1), and it was found to be greater than that of the non-ultrasonic synthesized catalyst (382 m2 g−1). The N2 adsorption-desorption isotherm indicated mesoporous structures. The SEM morphology at a similar magnification exhibited quite different shapes. In comparison to traditional methods, ultrasonic approaches produce higher yields in less time and use less energy. Furthermore, the effect of the preparation method of the 1%Pd-HPW/SiO2 catalyst was extensively studied with respect to the synthesis of octahydroquinazolinones. Excellent product yields, a fast reaction time, and simple work-up methods are some peculiarities associated with the ultrasonically synthesized catalyst. The recycling study was also investigated and found suitable for up to four reaction cycles.
Enhancement of Biological Pretreatment on Rice Straw by an Ionic Liquid or Surfactant
Authors: Chang Ken-Lin , Liu Chun-Hung , Phitsuwan Paripok , Ratanakhanokchai Khanok , Lin Yung-Chang , Dong Cheng-Di , Lin Ming-Hsun , Yang Gordon C. C.
Published: October 2021

Fungal delignification can be a feasible process to pretreat biomass for bioethanol production if its performance is improved in terms of efficiency through a few modifications. The aim of this study was to enhance the biodelignification pretreatment of rice straw using laccase in the presence of ionic liquid (1-Allyl-3-methylimidazolium chloride, [AMIM]Cl) or surfactant (TritonX-100). Addition of 750 mg/L [AMIM]Cl and 500 mg/L TritonX-100 increases the lignin removal to 18.49% and 31.79%, which is higher than that of laccase only (11.97%). The enzymatic saccharification process was carried out based on different strategies. The highest cellulose conversion, 40.96%, 38.24%, and 37.91%, was obtained after 72 h of enzymatic saccharification when the substrate was washed with distilled water after pretreatment of rice straw with laccase + TritonX-100, laccase + [AMIM]Cl, and laccase only, respectively. In addition, the morphology and structure changes of pretreated and untreated rice straw were studied. Both surface area and cellulose crystallinity are substantially altered after laccase + [AMIM]Cl and laccase + TritonX-100 pretreatment. Enhanced saccharification efficiency of rice straw was achieved by laccase pretreatment with ionic liquid or surfactant in a single system.
Fibroblast Memory in Development, Homeostasis and Disease
Authors: Kirk Thomas , Ahmed Abubkr , Rognoni Emanuel
Published: October 2021

Fibroblasts are the major cell population in the connective tissue of most organs, where they are essential for their structural integrity. They are best known for their role in remodelling the extracellular matrix, however more recently they have been recognised as a functionally highly diverse cell population that constantly responds and adapts to their environment. Biological memory is the process of a sustained altered cellular state and functions in response to a transient or persistent environmental stimulus. While it is well established that fibroblasts retain a memory of their anatomical location, how other environmental stimuli influence fibroblast behaviour and function is less clear. The ability of fibroblasts to respond and memorise different environmental stimuli is essential for tissue development and homeostasis and may become dysregulated in chronic disease conditions such as fibrosis and cancer. Here we summarise the four emerging key areas of fibroblast adaptation: positional, mechanical, inflammatory, and metabolic memory and highlight the underlying mechanisms and their implications in tissue homeostasis and disease.
Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells
Authors: Lee Mi Hee , Park Ye Jin , Hong Seung Hee , Koo Min-Ah , Cho Minyoung , Park Jong-Chul
Published: October 2021

Electrical stimulation is a well-known strategy for regulating cell behavior, both in pathological and physiological processes such as wound healing, tissue regeneration, and embryonic development. Electrotaxis is the directional migration of cells toward the cathode or anode when subjected to electrical stimulation. In this study, we investigated the conditions for enhanced directional migration of electrically stimulated adipose-derived stem cells (ADSCs) during prolonged culture, using a customized agar-salt electrotaxis chamber. Exposure of ADSCs to a 1200 μA electric current for 3 h, followed by cessation of stimulation for 6 h and resumed stimulation for a further 3 h, increased directional cell migration toward the anode without inducing cell death. Moreover, Golgi polarization maintained the direction of polarity parallel to the direction of cell movement. Herein, we demonstrated that a pulsed electric current is sufficient to trigger directional migration of ADSCs in long-term culture while maintaining cell viability.
COVID-19 in Pediatric Inpatients: A Multi-Center Observational Study of Factors Associated with Negative Short-Term Outcomes
Authors: Rubenstein Sara , Grew Emily , Clouser Katharine , Kwok Alexander , Veerapandiyan Aravindhan , Kornitzer Jeffrey , Pecor Keith , Ming Xue
Published: October 2021

Most cases of COVID-19 in children and adolescents are mild or asymptomatic, but a small number of individuals may develop severe disease, requiring PICU admission and/or mechanical ventilation. We assessed the factors associated with negative short-term outcomes of COVID-19 in 82 pediatric patients at three hospitals within the United States during the spring and summer of 2020 using medical records, laboratory data, and imaging studies of all patients admitted with a positive RT-PCR test for SARS-CoV-2. We found that older patients were more likely to have an extended hospital stay, and those with high BMIs (over 25) were more likely to be admitted to the PICU during the early pandemic. In addition, older patients, those with high BMIs, and those with underlying medical conditions, were more likely to receive respiratory support. Given the association of age, BMI, and underlying medical conditions with more severe COVID-19, clinicians should keep these factors in mind when treating patients.
Non-Thermal Reactive N2/He Plasma Exposure to Inhibit Epithelial Head and Neck Tumor Cells
Authors: Wu Chih-Ying , Liao Jiunn-Der , Chen Chang-Han , Lee Han , Wang Shyh-Hau , Liu Bernard Haochih , Lee Cheng-Yi , Shao Pei-Lin , Li Enya
Published: October 2021

The traditional therapy for head and neck cancer patients has several side effects. Hence, regular follow-up care is usually required. Recently, non-thermal micro-plasma was applied to inactivate cancer cells. Such a physical method provides localized energy and reactive oxygen/nitrogen species (ROS/RNS). In this study, the ability of non-oxygen N2/He micro-plasma to inactivate four pharynx squamous carcinomatous cells, namely SAS, CAL 27, FaDu, and Detroit 562, under different exposure durations is evaluated. The four cell lines were affected with regard to proliferation, reduction, and apoptosis-related DNA damage, implying that the cell medium is critical in plasma–cell interaction. This is expected to be a promising method for head and neck cancer cell suppression through plasma-initiated ROS/RNS species under a suitable exposure time.
Optimization of Quantitative Analysis of Biofilm Cell from Pipe Materials
Authors: Papciak Dorota , Domoń Andżelika , Zdeb Monika , Skwarczyńska-Wojsa Agata , Konkol Janusz
Published: October 2021

The quantitative analysis of biofilm can be used not only to assess the microbiological stability of tap water but also on its basis can assess: the degree of colonization of materials by bacterial cells, the rate of biofilm formation on the surfaces of pipes and determine their composition and number. The article presents the results of research on the development of an effective method of biofilm detachment from the surface of the galvanized steel. The number of biofilm cells was determined by methods: (1) luminometric ATP determination, (2) flow cytometry and (3) heterotrophic plate counts (HPC). The presence of the biofilm was confirmed by SEM and fractal analysis. The analysis of the obtained results showed that the most effective method of detaching the biofilm cells from the galvanized steel surface was the mechanical separation with a sterile cotton swab. The variant with the use of a sterile swab enables rapid collection of the biofilm from the surface of the ducts forming internal installations or water supply networks. Due to the simplicity and speed of obtaining results, the luminometric ATP measurement has been established as the best method for the quantification of biofilm cells. The results of this study were intended to provide reliable and useful data on the quantification of biofilm cells.
Morphological: Optical, and Mechanical Characterizations of Non-Activated and Activated Nanocomposites of SG and MWCNTs
Authors: Alotaibi Mohammed , Almousa Norah , Asaker Mohammed , Alkasmoul Fahad , Khdary Nezar , Khayyat Maha
Published: October 2021

Nanocomposites of silica gel (SG) and multiwalled carbon nanotubes (MWCNTs) of relatively low concentrations (0.25, 0.50, and 0.75 wt%) were characterized before and after annealing. Adsorption is a surface phenomenon, and based on this, the morphology of the composites was investigated by scanning electron microscopy (SEM). The produced images show that the MWCNTs were embedded into the silica gel base material. Fourier transform infrared (FTIR) transmittance spectroscopy showed that MWCNTs were not functionalized within the matrix of silica gel and MWCNT composites. However, after annealing the composites at 400 °C for 4 h in air, evidence of activation was observed in the FTIR spectrum. The effects of the embedding of MWCNTs on porosity, specific surface area, and pore size distribution were studied using Raman spectroscopy. The Raman spectra of the prepared composites were mainly dominated by characteristic sharp scattering peaks of the silica gel at 480, 780, and 990 cm−1 and a broad band centered at 2100 cm−1. The scattering peaks of MWCNTs were not well pronounced, as the homogeneity of the composite is always questionable. Nanosizer analysis showed that at 0.25 wt%, the distribution of MWCNTs within the silica gel was optimal. Vickers hardness measurements showed that the hardness increased with the increasing weight percent of MWCNTs within the composite matrix, while annealing enhanced the mechanical properties of the composites. Further studies are required to investigate the pore structure of silica gel within the matrix of MWCNTs to be deployed for efficient cooling and water purification applications.
Time-Resolved and Temperature-Dependent Fractional Amplitude Contributions to the Broadband Emission of CdSe Quantum Dots
Authors: Rice Quinton , Raut Sangram , Burney Kyle , Gryczynski Zygmunt , Gryczynski Ignacy , Yu William , Tabibi Bagher , Seo Jaetae
Published: October 2021

The broadband spontaneous emission of excitons in CdSe quantum dots (QDs) is of great interest for the spectral imaging of living organisms or specific substances in the visible spectral region as well as in the biological optical window near the infrared spectral region. Semiconductor QDs that are near the bulk Bohr radius exhibit wide spectral tunability and high color purity due to quantum confinement of excitons within the dot boundary. However, with reducing dot size, the role of the surface-trapped state increases. The temperature-dependent photoluminescence (PL) confirms this with a ~3:1 emission intensity decrease from the surface-trapped state compared to the band edge. Large crystal irregularity, dangling ions, and foreign molecules can introduce new electronic transitions from surface-trapped states that provide broad spontaneous emission in the spectral region from visible to near IR in addition to the band edge emission. The time-resolved PL analyzed the fractional contributions of band edge, surface-trapped states, and possible intermediate trapped states to the broad spectral emission in order to characterize the CdSe QDs.
Qualitative Characters of Indigenous Sheep in Central Brazil: Putting Phenotype into Perspective
Authors: Aranda Allana Novais , Silva Marcelo Corrêa da , Crispim Bruno do Amaral , Ledesma Luana Liz Medina , Lenis Patrícia Roseti , Silva Adrielly Lais Alves da , Leonardo Ariadne Patrícia , Vargas Junior Fernando Miranda de , Barufatti Alexeia
Published: October 2021

For centuries, indigenous sheep have been bred in extensive low-input systems in Midwestern Brazil. The hypothesis of this study was the assumption that phenotypic evaluation of indigenous livestock may drive the establishment of breed standards and official breed recognition, and, therefore, promote more local business opportunities. On the basis of more integrative and participatory theoretical background to applying any decision based on phenotype, we designed this research to determine the most typical and unusual phenotypes of Pantaneiro sheep. Pantaneiro ewes (281 ewes from five conservation units in five counties) were evaluated, bearing in mind both conservation and development. Descriptive statistics were used to classify ewes into typical, intermediate, and unusual phenotypes. Chi-squared tests for association were performed to test if morphological variation in the different sampling sites occurred randomly (p > 0.05) or not (p < 0.05). Some results suggest some sort of diversifying selection pressure, i.e., distinct preferences among keepers. We observed considerable morphologic variation among ewes, but it was straightforward to determine the predominant phenotypes. The study evokes integrative agrobiodiversity by putting phenotypical characterization of indigenous livestock into perspective. Discussions coming from this study may support innovative governance and participative decision-making, allied with strategies that value the socioeconomic, biocultural, and adaptive aspects of indigenous livestock in tropical regions and developing countries. This is a challenge for government, rare-breed keepers, value-chain actors, and civil society.