bluuebunny/crossref_metadata_2025 huggingface dataset:
Catheter-Directed Thrombolysis for Acute SVC Obstruction in a Patient with Metastatic Breast Cancer
Authors: Carroll Hailey , Dineen Fergal , Ryan Paul , Crowley Maeve , Power Stephen , O'Reilly Seamus
Published: July 2022

Background: External compression, thrombosis, or stenosis of the superior vena cava can lead to superior vena cava syndrome, a diagnosis that should be considered swiftly in patients presenting with classic symptoms such as facial and neck swelling, plethora, and distended neck veins.
Case Presentation: We report a case of acute port-a-cath associated superior vena cava thrombosis in a longstanding, previously uncomplicated vascular access device in a patient with stable ER+/PR+/HER2+ metastatic breast cancer. After initial treatment, there was limited clinical improvement with subcutaneous low molecular weight heparin (LMWH). Following multidisciplinary team discussion, catheter-directed thrombolysis was performed, which resulted in complete symptom resolution.
Conclusion: The recognition of signs and symptoms is crucial in diagnosing acute superior vena cava syndrome, particularly in patients with a malignancy history or a central venous access device in situ. The thrombotic complications of port-a-cath symptoms can occur at any time and management should be guided by multidisciplinary discussion. In appropriately selected patients, catheter-directed thrombolysis can be successful and can lead to rapid symptom resolution.
Implementation of Risk Management and Capital Structure on Financial Performance: A Study of Islamic Banking in Indonesia
Authors: Nihayah Ida , Aryani Y. Anni
Published: July 2022

This study examines the influence between risk management and capital structure on the financial performance of Islamic Commercial Banks (BUS) in Indonesia with 3 measurement proxies, namely ROA, ROE, and zakat. ROA's financial performance will be used as a key proxy in this study. Meanwhile, ROE and zakat will be used as additional proxies to see the difference in results between the three of them. Risk management is measured by parameters of credit/financing risk management (NPF), liquidity risk (FDR), and operational risk (BOPO). Meanwhile, capital structure is measured using the ratio of CAR and DER. This study also added five control variables: company size, income stability, asset growth, third-party funds, and financing. This study used panel data and purposive sampling techniques to determine the sample. Data obtained is on published financial statements of Islamic commercial banks for 2008-2019. The data analysis technique used is a multiple regression analysis technique using E-views 9 program. The results showed that BOPO and CAR variables affected financial performance (ROA), while NPF, FDR, and DER have no effect on financial performance (ROA), and there were inconsistencies in results with ROE and Zakat proxies.
Description Quality of Sleep of Children With Autism
Authors: Kulsum Dewi Umu , Oktavia Oktavia
Published: July 2022

Background: A developmental disorder that affects communication and behavior is known as Autism. The prevalence of autism is still increasing, ranging from 1-2/1000 in males more than in females (4:1). In children with autism, physical health problems are often complained of, one of which is sleep problems, such as delayed sleep onset, frequent awakenings at night, and sleep duration. This problem makes parents stressed because it impacts the severity of behavior both during the day and at night.
Objectives: The description of sleep quality in children with autism.
Methods: Cross-sectional approach to 55 special school students with autism in Cimahi. Data collection was collected by measuring sleep quality with the Children's Sleep Habits Questionnaire (CSHQ) with a cut-off point of < 30 good sleep quality and > 30 sleep problems with Chronbach's alpha = 0.68 to 0.78. Content validity; ROC = 0.41, differentiating between clinical and control groups; sensitivity = 0.80, specificity = 0.72. Data analysis was carried out univariate (frequency distribution) and bivariate with a chi-square test. 
Results: It was found that most of the children with moderate Autism had sleep problems (54.5%) p = 0.005.
Conclusion: The study results describe that some children with autism in the city of Cimahi have sleep problems in conditions of delayed sleep onset and often wake up at night.
Application of Progressive Muscle Relaxation Therapy in Overcoming Nurse's Work Stress in Depok City Regional General Hospitals
Authors: Siregar Tatiana , Rabani Andal Izah
Published: July 2022

Background: Every nurse who works in a hospital will face quite time-consuming and laborious work, especially when the bed occupancy rate (BOR) is 85%, and the work pressure of nurses is high. The effect of work stress manifests as physical fatigue, psychological changes, and behavioral changes. Nurses need to try to prevent work stress from happening for a long time by doing stress management, one of which is progressive muscle relaxation therapy.
Objectives: The purpose of the study was to determine the effect of progressive muscle relaxation therapy in reducing nurses’ work stress.
Methods: The study was conducted on five resident nurses who were trained using Progressive Muscle Relaxation and then used the  Nursing Stress Scale to measure stress levels before and after training. The research design was Quasi Experiment with One group pretest and post-test designs;  data analysis with paired t-test.
Results: The results showed that there was a significant effect of implementing progressive muscle relaxation therapy on reducing nurse work stress (p = 0.024; 95% CI).
Conclusion: There is a significant effect of progressive muscle relaxation therapy on the work stress response of nurses. Progressive muscle relaxation therapy is good to apply to hospital nurses who go through  work stress so that stress does not occur which can risk reducing the quality of service to patients.
Storytelling Therapy Effectively Reduces Anxiety in Children with Leukemia at DR Wahidin Sudirohusodo General Hospital Makassar
Authors: Haristiani Ruris , Wahyuningsih Wahyuningsih , Susaldi Susaldi
Published: July 2022

Background: Children with leukemia are prone to experience anxiety due to various factors. Moreover, side effects of chemotherapy such as diarrhea, nausea, and vomiting add stress to the children. Storytelling therapy can be used as one of the non-pharmacological methods to reduce anxiety in children.
Objectives: seeing the effectiveness of storytelling therapy with audio-visual media in reducing anxiety in children with leukemia
Methods: quantitative research with quasi-experimental: pretest-posttest without a control group. Anxiety in children is measured by Hamilton Anxiety Rating Scale (HARS) instrument. Statistical test with the Wilcoxon signed-rank test with α = 0,05
Results: There is a decrease in anxiety levels in children with leukemia after being given story-telling therapy. Based on data analysis by the Wilcoxon test, p: 0.000 means story-telling therapy is effective in reducing anxiety in children with leukemia at RSUP DR Wahidin Sudiro Husodo Hospital Makassar
Conclusion: Storytelling therapy with audio-visual media is effective in reducing anxiety levels in children with leukemia
Comparison the Efectiveness of Autogenic Relaxation and Deep Breath Relaxation on Fatigue Value in Renal Failure Patients During Hemodyalisis
Authors: Utami Indhit Tri , Dewi Tri Kesuma , Nurhayati Sri , Annisa Fitri
Published: July 2022

Background: Fatigue is one of the problems with a fairly high prevalence among the effects of hemodialysis which hurt the quality of life. Fatigue can be done with relaxation. Relaxation can be done with autogenic and deep breath relaxation.
Objectives: This study aims to compare the effectiveness of Autogenic Relaxation and Deep Breathing Relaxation on the Fatigue Value of Chronic Kidney Failure Patients Undergoing Hemodialysis.
Methods: This study used a quasi-experimental two-group randomized pre-post test design. The sample in this study amounted to 40 respondents in total sampling. Bivariate analysis using independent t-test.
Results: The results showed that there was no difference in the results between autogenic relaxation and deep breathing relaxation.
Conclusion: Autogenic relaxation and deep breathing relaxation are equally effective in reducing fatigue in renal failure patients undergoing hemodialysis
MOLIÈRE’İN TİYATROSUNDA “BARBON”
Authors: ETENSEL İLDEM Şerife Arzu
Published: July 2022

2022 yılında doğumunun 400. yılını kutladığımız Molière’in komedyalarında karşımıza çıkan “barbon” kalıp kişisi Antik Roma’da doğmuş, Attelan farslarında önemli bir yer edinmiş ve Commedia dell’arte/İtalyan Komedyasının başat kalıp kişilerinden biri olmuştur. Antik Roma komedyasındaki “senex”, Attelan farsında “pappus”, Commedia dell’arte’de “pantalone” adını almış, Molière’in oyunlarında “barbon” olarak tanınmıştır. Barbonlar Molière’in çeşitli fars ve komedyalarında yer almaktadır. Bunlar: L’Etourdi ou les comntretemps/Şaşkın ya da Aksilikler, l’Ecole des maris/Kocalar Okulu, l’Ecole des femmes/Kadınlar Okulu, le Mariage forcé/Zorla Evlilik, George Dandin, l’Avare/Cimri ve Le Malade imaginaire/Hastalık Hastası oyunlarıdır. Bir kalıp kişi olarak barbonun karakter özellikleri tüm oyunlarda aşağı yukarı aynıdır: abartılı özgüven, budalalık, cinsellik merakı, kıskançlık ve aldatılma korkusu, despotluk ve cimrilik. Barbon kadınların kendisini nasıl gördüğünü anlamaz, onların gözünde çekici ve beğenilen bir erkek olduğunu düşünür. Oysa kadınların tek amacı barbonun parasını ele geçirmektir. Molière barbon kalıp kişisini kullanarak babaların ve vasilerin çocuklarının evlenmesi konusundaki tutumlarını da eleştirmiş, genç kızların eğitimiyle ilgili düşüncelerini ifade etmiştir. Molière barbonun gülünç kişiliğinden yararlanarak eleştirilerini olabildiğince kolay bir biçimde dile getirmiştir.
ON CENTRAL AUTOMORPHISMS OF FREE METABELIAN LIE ALGEBRAS
Authors: ERGİNKARA Başak , FINDIK Şehmus
Published: July 2022

Let $F_m$ be the free metabelian Lie algebra of rank $m$ over a field $K$ of characteristic 0. An automorphism $\varphi$ of $F_m$ is called central if $\varphi$
commutes with every inner automorphism of $F_m$. Such automorphisms form the centralizer $\text{\rm C}(\text{\rm Inn}(F_m))$
of inner automorphism group $\text{\rm Inn}(F_m)$ of $F_m$ in $\text{\rm Aut}(F_m)$. We provide an elementary proof to show that $\text{\rm C}(\text{\rm Inn}(F_m))=\text{\rm Inn}(F_m)$.
Bioefficacy of Adansonia digitata L. (BAOBAB) extract against aflatoxin produced by Aspergillus on Sesamum indicum L. (Sesame) seeds
Authors: Addy Jose , Victory Ugocha
Published: July 2022

The leaf and bark extracts of Adansonia digitata were used as inhibition agents to curtail the spread of aflatoxin produced by Aspergillus niger on sesame seeds. Susceptibility test was carried out using agar well diffusion method. The ethanoic and aqueous leaf and bark extracts of Adansonia digitata were subjected to phytochemical screening using standard methods of analysis and this investigation revealed the presence of tannins, flavonoids, saponins, glycosides, alkaloids, quinones and phenols. The test organism isolated from sesame seeds was identified as Aspergillus niger. The extracts at different concentrations 100mg/ml, 125 mg/ml, 200 mg/ml, 250 mg/ml and 500 mg/ml showed varied inhibitory activity against the fungus studied with ethanoic leaf extract having the highest inhibition at concentration of 500mg/ml with mean inhibition zone of 35.00±1.00. Ampicillin (250 mg) was used as a positive control. It is therefore recommended that bio-preservation of benniseed using Adansonia digitata be considered to avoid infestation by Aspergillus niger in humid and insect infested areas.
Response of mungbean to foliar application of nitrogen and micronutrients during reproductive stages
Authors: Das Utpal Kumar , Mondal M. Monjurul Alam
Published: July 2022

An experiment was conducted at the pot yard of Bangladesh Institute of Nuclear Agriculture, Mymensingh during the period from February to May 2017 to investigate the effect of foliar application of nitrogen and micronutrients on crop characters, yield attributes and yield of two mungbean genotypes. The experiment comprised four levels of nutrients foliar application like, i) T1 = Control; ii) T2 = Foliar application of urea at the rate of 1.5% four times from flowering start to pod development stage with an interval of 4 days; iii) T3 = T2 + 0.1% micronutrients (B, Mo, Zn, Mn, Ca, Fe, Cu) four times from flowering start to pod development stage with an interval of 4 days and iv); T4 = T3 + side dressing (soil application) of 1.5% N and 0.1% micronutrients solution four times from flowering start to pod development stage with an interval of 4 days. The genotypes were Binamoog-6 and Binamoog-7. Results revealed that morphological (plant height, branch and leaf number, leaf area plant-1), physiological (total dry mass plant-1, specific leaf weight, chlorophyll), yield attributes (number of pods plant-1, pod length, single pod weight, number of seeds pod-1 and 100-seed weight) and yield increased in foliar nutrients applied plants over control but the increment was greater in T2 and T3 than the T4 treatment. The highest plant height, branch and leaf number, leaf area, total dry mass, pod number, pod length, 100-seed weight and seed yield were recorded in T2 followed by T3 with same statistical rank (in most cases). The lowest morpho-physiological, yield attributes and yield were recorded in T1 (control) plants. Binamoog-7 was superior in most of plant parameters and yield compared to Binamoog-6.
A systematic review on nanomedicine
Authors: Nawaz Yasir , Nazar Ishart , Hakeem Luqman , Saddiqa Ayesha , Hakeem Luqman , Ahmad Hassnain , Khan Muhammad Saleem , Ullah Muhammad Kaleem , Shafiaq Hafiza Rabia , Iqbal Khadija , Waijad Muhammad , Saeed Bilal
Published: July 2022

Nanomedicine is used to cure cancer of many tissues such as ovarian cancer and multiple myeloma. With the help of nanotechnology, the probability of failure or rejection of a transplant, an organ is reduced. Nanomedicine is one of the latest and better fields which is used for the treatment of tumors. Some of the methods used to produce nanoparticles are discussed here. Many nanoparticles are used for the benefit of humans. The solvent is subsequently dispersed in a surfactant's aqueous media, resulting in the formation of a film in an instant colloidal suspension called solvent displacement. This method can be used to achieve a pharmacological payload. Supersaturation and precipitation are required for the assembly and stability of drug nanoparticles. Shear and cavitation forces are also used to produce nanoparticles. The milling media is generating shear forces on impact, resulting in nanoparticles, the principle of size reduction. Extrusion is also used to generate nanoparticles. Hydrophobic drugs are converted directly into nanoparticles. The capacity to build nanocores with a wide range of functionalities is another key advantage of the HAylation process (a method to make nanoparticles). Determine medication, metabolism and hepatotoxicity in these models in nanomedicine have the potential to have a significant influence in the field. Red blood cells (RBCs), for example, are sensitive to substantial external flow pressures, and their inherent formability can be used as a biomarker to identify a range of RBC diseases. It is concluded that different nanoparticles are made to treat different kinds of diseases and to make different therapies for cancer etc. Now a day’s nanotechnology is the method that uses nanoparticles to cure diseases and provide a significant treatment approach to humans.
Gamma irradiation effect on morphological character of M1 generation of okra
Authors: Rouf Md. Abdur , Tasnim Sadia , Khan Nazmul Alam , Sarkar Mohammad Joyel , Sumi Mirana Akhter , Nabi K.M. Eadun , Farthouse Jannatul
Published: July 2022

The study was done to assess the impact of various dosages of gamma beams (150, 300, 450 and 600Gy and zero portions as control) on different morphological parts of two assortments of Abelmoschus esculentus named BARIdherosh-1 and BARIdherosh-2. The experiment was spread out in a randomized complete block design (RCBD) with three replications. The consequences of various portions showed that gamma illumination essentially influences all boundaries i.e., plant height, number of leaves per plant, number of pods per plant, length of pod and number of seeds per plant. Plant height was significantly increased at 600 Gy (133.6cm) and 150 Gy (155.2cm) for BARIdherosh-1 and BARIdherosh-2 respectively as compared to control. At 300Gy (16.35) and 450Gy (16.23), maximum pods per plant were found for BARIdherosh-1 and BARIdherosh-2. For BARIdherosh-1 maximum number of seeds per plant (590.17) was observed at 150 Gy and for BARIdherosh-2 (542.33) it was 450 Gy.
Surfactant-assisted alkaline pretreatment and enzymatic hydrolysis of Miscanthus sinensis for enhancing sugar recovery with a reduced enzyme loading
Authors: Cheng Xiyu , Luo Ying , Gao Yifan , Li Shen , Xu Chunming , Tang Shangyuan , Yang Yongkun , Zhang Zehua , Jiang He , Xu Hanli , Shi Shuobo , Yan Qiong
Published: July 2022

Surfactants play a vital role in the delignification and saccharification of lignocellulosic biomass. A strategy for coupling surfactant-assisted alkaline pretreatment (SAP) with surfactant-assisted enzymatic hydrolysis (SEH) has been proposed for improving sugar recovery from a potential energy crop, Miscanthus sinensis. Poly (ethylene glycol) 2000 (PEG 2000) was found to be more efficient in SAP than in other tested surfactants. Compositional and structural analysis revealed that the SAP process with 1% of PEG 2000 produced more efficient lignin removal and microstructure disruption of the pretreated sample, thus indicating much higher reducing sugar yields of 544.4–601.2 mg/g compared to the samples that were untreated or pretreated by alkali alone. Moreover, SEH with 1% Tween 80, which could block the lignin-enzyme interactions, produced a substantial reduction of 33.3% in the enzyme loading to achieve a higher sugar recovery from the SAP sample.
A switchable light-responsive azopolymer conjugating protein micropatterns with topography for mechanobiological studies
Authors: Cimmino Chiara , Netti Paolo A. , Ventre Maurizio
Published: July 2022

Stem cell shape and mechanical properties in vitro can be directed by geometrically defined micropatterned adhesion substrates. However, conventional methods are limited by the fixed micropattern design, which cannot recapitulate the dynamic changes of the natural cell microenvironment. Current methods to fabricate dynamic platforms usually rely on complex chemical strategies or require specialized apparatuses. Also, with these methods, the integration of dynamic signals acting on different length scales is not straightforward, whereas, in some applications, it might be beneficial to act on both a microscale level, that is, cell shape, and a nanoscale level, that is, cell adhesions. Here, we exploited a confocal laser-based technique on a light-responsive azopolymer displaying micropatterns of adhesive islands. The laser light promotes a directed mass migration and the formation of submicrometric topographic relieves. Also, by changing the surface chemistry, the surfacing topography affects cell spreading and shape. This method enabled us to monitor in a non-invasive manner the dynamic changes in focal adhesions, cytoskeleton structures, and nucleus conformation that followed the changes in the adhesive characteristic of the substrate. Focal adhesions reconfigured after the surfacing of the topography, and the actin filaments reoriented to coalign with the newly formed adhesive island. Changes in cell morphology also affected nucleus shape, chromatin conformation, and cell mechanics with different timescales. The reported strategy can be used to investigate mechanotransduction-related events dynamically by controlling cell adhesion at cell shape and focal adhesion levels. The integrated technique enables achieving a submicrometric resolution in a facile and cost-effective manner.
Gut Microbiota Correlates With Clinical Responsiveness to Erythropoietin in Hemodialysis Patients With Anemia
Authors: Zhu Yifan , Tang Yuyan , He Haidong , Hu Ping , Sun Weiqian , Jin Meiping , Wang Lishun , Xu Xudong
Published: July 2022

The main treatment for renal anemia in end-stage renal disease (ESRD) patients on hemodialysis is erythropoiesis (EPO). EPO hyporesponsiveness (EH) in dialysis patients is a common clinical problem, which is poorly understood. Recent searches reported that gut microbiota was closely related to the occurrence and development of ESRD. This study aims to explore the changes in gut microbiota between ESRD patients with different responsiveness to EPO treatment. We compared the gut microbiota from 44 poor-response (PR) and 48 good-response (GR) hemodialysis patients treated with EPO using 16S rDNA sequencing analysis. The results showed that PR patients displayed a characteristic composition of the gut microbiome that clearly differed from that of GR patients. Nine genera (Neisseria, Streptococcus, Porphyromonas, Fusobacterium, Prevotella_7, Rothia, Leptotrichia, Prevotella, Actinomyces) we identified by Lasso regression and ROC curves could excellently predict EH. In contrast, five genera (Faecalibacterium, Citrobacter, Bifidobacterium, Escherichia–Shigella, Bacteroides) identified by the same means presented a protective effect against EH. Analyzing the correlation between these biomarkers and clinical indicators, we found that gut microbiota may affect response to EPO through nutritional status and parathyroid function. These findings suggest that gut microbiota is altered in hemodialysis patients with EH, giving new clues to the pathogenesis of renal anemia.
GDF15 and ACE2 stratify COVID-19 patients according to severity while ACE2 mutations increase infection susceptibility
Authors: Torrens-Mas Margalida , Perelló-Reus Catalina M. , Trias-Ferrer Neus , Ibargüen-González Lesly , Crespí Catalina , Galmes-Panades Aina Maria , Navas-Enamorado Cayetano , Sanchez-Polo Andres , Piérola-Lopetegui Javier , Masmiquel Luis , Crespi Lorenzo Socias , Barcelo Carles , Gonzalez-Freire Marta
Published: July 2022

Coronavirus disease 19 (COVID-19) is a persistent global pandemic with a very heterogeneous disease presentation ranging from a mild disease to dismal prognosis. Early detection of sensitivity and severity of COVID-19 is essential for the development of new treatments. In the present study, we measured the levels of circulating growth differentiation factor 15 (GDF15) and angiotensin-converting enzyme 2 (ACE2) in plasma of severity-stratified COVID-19 patients and uninfected control patients and characterized the in vitro effects and cohort frequency of ACE2 SNPs. Our results show that while circulating GDF15 and ACE2 stratify COVID-19 patients according to disease severity, ACE2 missense SNPs constitute a risk factor linked to infection susceptibility.
Time-Varying Effect of Ductile Flexural Toppling Failure on Antidip Layered Rock Slope
Authors: Cai Junchao , Zheng Da , Ju Nengpan , Wang Jue , Zhou Xin , Li Da
Published: July 2022

Ductile flexural toppling failure is a common form of toppling failure, and it is the product of long-term geological history and shows the characteristics of long-term deformation and progressive failure. The creep characteristics of rock mass have been seldom considered in the current research on toppling, especially interlayer creep of rock layers in the process of toppling. Based on the thought of deformation stability analysis, the flexural toppling failure was divided into the following four stages: start-up, rapid deformation, transient stability, and long-term creep stages. Combined with mechanical analysis, the developmental conditions of the start-up, transient stability, and long-term creep development stages are discussed respectively. Finally, several cases were selected to analyze the stage and the stability of the toppling deformation body, as well as to verify the rationality of the mechanical analysis condition. Study results show that the start-up conditions meet Equation 2, the rock layer inclination and slope angle is 0.5π−φ in the transient stability stage, and that angle is the infinite 0.5π in the long-term creep stage. Other external forces (such as water pressure) will intensify the development of ductile flexural toppling failure, so that the angle between the toppled bedding surface and the slope surface increases. It is of great significance to analyze the development stage of the ductile flexural toppling, comprehensively analyze and evaluate the stability of the ductile flexural toppling, reasonably develop and utilize its self-stability ability, and set up support measures.
Altered Regional Homogeneity in Patients With Diabetic Erectile Dysfunction: A Resting-State fMRI Study
Authors: Chen Jianhuai , Huang Xinfei , Tang Qinglai , Xiang Ziliang , Xu Yan , Liu Tao , Yang Zhaoxu , Yang Jie , Chen Yun
Published: July 2022

Introduction

Erectile dysfunction (ED) is a common complication of Type-2 Diabetes Mellitus (T2DM) for male patients and it is considered to be associated with different causes including hyperglicemia-induced vascular endothelial cell damages. However, the possible central neural mechanisms shared by these two diseases remain unclear. This study aimed to explore the changes of brain activity and their relationships with the clinical characteristics in patients with diabetic ED.

Methods

The data of resting-state functional magnetic resonance imaging were acquired in 31 T2DM patients with ED (DM-ED) and 31 matched healthy controls (HCs). The whole-brain regional homogeneity (ReHo) values were calculated and compared between groups. In addition, Pearson correlation analysis was performed to evaluate the relationships between brain regions with altered ReHo values and clinical characteristics in the patient group.

Results

The DM-ED group exhibited increased ReHo values in the right middle frontal gyrus (orbital part) and decreased ReHo values in the left superior frontal gyrus (dorsolateral), paracentral lobule, precuneus and bilateral supplementary motor area when compared with the HCs group. Moreover, significantly negative correlations were found between ReHo values of the left superior frontal gyrus (dorsolateral) and IIEF-5 scores, as well as the level of HbA1c in the DM-ED group.

Conclusion

The altered spontaneous brain activity in cognitive-related regions revealed by ReHo values might provide new insights into the neurological pathophysiology underlying DM-ED and serve as potential neuroimaging biomarkers for detecting and evaluating ED in diabetes patients.
An Individualized Recommendation for Controlled Ovary Stimulation Protocol in Women Who Received the GnRH Agonist Long-Acting Protocol or the GnRH Antagonist Protocol: A Retrospective Cohort Study
Authors: Chen Ming-Xing , Meng Xiang-Qian , Zhong Zhao-Hui , Tang Xiao-Jun , Li Tian , Feng Qian , Adu-Gyamfi Enoch Appiah , Jia Yan , Lv Xing-Yu , Geng Li-Hong , Zhu Lin , He Wei , Wan Qi , Ding Yu-Bin
Published: July 2022

Background

The GnRH agonist long-acting protocol and GnRH antagonist protocol are widely used in ovarian stimulation. Which protocol eliciting higher live birth rate for IVF/ICSI patients with different ages, different ovarian reserves and different body mass index (BMI) has not been studied. However, among these protocols, the one that elicits higher live birth in IVF/ICSI patients with different ages, ovarian reserves and body mass indexes (BMI) has not been identified.

Methods

This was a retrospective cohort study about 8579 women who underwent the first IVF-ET from January, 2018 to August, 2021. Propensity Score Matching (PSM) was used to improve the comparability between two protocols.

Results

After PSM, significant higher live birth rates were found in the GnRH agonist long-acting protocol compared to GnRH antagonist protocol (44.04% vs. 38.32%) (p<0.001). Stratified analysis showed that for those with AMH levels between 3 ng/ml and 6 ng/ml, with BMI ≥ 24 kg/m2 and were aged ≥ 30 years old, and for those women with BMI < 24kg/m2 and were aged ≥30 years whose AMH levels were ≤ 3ng/ml, the GnRH agonist long-acting protocol was more likely to elicit live births [OR (95%CI), 2.13(1.19,3.80)], [OR (95%CI), 1.41(1.05,1.91)]. However, among women with BMI ≥ 24kg/m2 and were aged ≥30 years whose AMH levels were ≤ 3ng/ml, the GnRH agonist long-acting protocol had a lower possibility of eliciting live births [OR (95%CI), 0.54(0.32,0.90)]. Also, among women with AMH levels between 3 ng/ml and 6 ng/ml, with BMI ≥ 24 kg/m2 and with age < 30 years and for those with AMH levels between 3 ng/ml and 6 ng/ml, regardless of age, and with BMI<24kg/m2,, the possibility of live births was similar between the two protocols [OR (95%CI), 1.06(0.60,1.89)], [OR (95%CI), 1.38(0.97,1.97)], [OR (95%CI), 0.99(0.72,1.37)]. Among the women with AMH levels ≤ 3 ng/ml and with were aged < 30years, regardless of BMI, the possibility of live birth was similar between the two protocols [OR (95%CI), 1.02(0.68,1.54)], [OR (95%CI), 1.43(0.68,2.98)]. Moreover, among women with AMH levels ≥ 6ng/ml, the possibility of live birth was similar between the two protocols [OR (95%CI),1.42(0.75,2.69)], [OR (95%CI),1.02(0.19,5.35)], [OR (95%CI), 1.68(0.81,3.51)], [OR (95%CI), 0.51(0.10,2.55)].

Conclusions

The suitability of the GnRH agonist long-acting protocol or GnRH antagonist protocol to infertility patients is dependent on specific biological characteristics of the patients.
Grapevine nursery propagation material as source of fungal trunk disease pathogens in Uruguay
Authors: Carbone María Julia , Gelabert Matías , Moreira Victoria , Mondino Pedro , Alaniz Sandra
Published: July 2022

Grapevine fungal trunk diseases (GTDs) have become a serious problem for grapevines worldwide. Nursery vines infected during the propagation process are considered one of the main ways of dissemination of GTD pathogens. In this study, we examined the status of GTDs in grapevine planting material, from rootstocks and scion mother cuttings to grafted rooted vines ready to plant, according to the local nursery propagation process. During 2018-2019, internal symptoms of GTDs were examined in 2400 propagation materials and fungal isolations were carried out from a subsample of 1026 selected materials. Our results revealed that nursery grapevine plants produced in Uruguay have a high incidence of GTDs, regardless of the scion/rootstock combination. Typical brown to black streaks and sectorial wood necrosis were observed in materials on all propagation stages, with a markedly increasing incidence throughout the nursery process, reaching almost 100% in grafted rooted vines ready to plant. Botryosphaeria dieback, Petri disease and black-foot disease were the main GTDs found. The results showed that Botryosphaeria dieback and Petri disease pathogens infect materials from the early stages of the process, with a marked increase towards the end of the plant production process, whereas black-foot disease pathogens were found exclusively in vines ready to plant. Diaporthe dieback pathogens were also detected in materials in all stages but in a low proportion (less than 10% of infected material). Based on single locus analysis, the 180 isolates selected were placed into eight genera and 89% identified within 22 fungal species associated with GTDs, with Phaeoacremonium oleae and Diaporthe terebinthifolii as new records on grapevine worldwide. Our results have concluded that locally produced vines are one of the main ways of dissemination of GTD pathogens and showed that a nursery sanitation programme is required to reduce the incidence of these diseases.
Sequence similarity of SARS-CoV-2 and humans: Implications for SARS-CoV-2 detection
Authors: Li Heng , Hong Xiaoping , Ding Liping , Meng Shuhui , Liao Rui , Jiang Zhenyou , Liu Dongzhou
Published: July 2022

Detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) needs human samples, which inevitably contain trace human DNA and RNA. Sequence similarity may cause invalid detection results; however, there is still a lack of gene similarity analysis of SARS-CoV-2 and humans. All publicly reported complete genome assemblies in the Entrez genome database were collected for multiple sequence alignment, similarity and phylogenetic analysis. The complete genomes showed high similarity (>99.88% sequence identity). Phylogenetic analysis divided these viruses into three major clades with significant geographic group effects. Viruses from the United States showed considerable variability. Sequence similarity analysis revealed that SARS-CoV-2 has 612 similar sequences with the human genome and 100 similar sequences with the human transcriptome. The sequence characteristics and genome distribution of these similar sequences were confirmed. The sequence similarity and evolutionary mutations provide indispensable references for dynamic updates of SARS-CoV-2 detection primers and methods.
No bidirectional relationship between depression and periodontitis: A genetic correlation and Mendelian randomization study
Authors: Nolde Michael , Holtfreter Birte , Kocher Thomas , Alayash Zoheir , Reckelkamm Stefan Lars , Ehmke Benjamin , Baurecht Hansjörg , Baumeister Sebastian-Edgar
Published: July 2022

Background

Observational and in-vivo research suggested a bidirectional relationship between depression and periodontitis. We estimated the genetic correlation and examined directionality of causation.

Methods

The study used summary statistics from published genome wide association studies, with sample sizes ranging from 45,563 to 797,563 individuals of European ancestry. We performed linkage disequilibrium score regression (LDSC) to estimate global correlation and used Heritability Estimation from Summary Statistics (ρ-HESS) to further examine local genetic correlation. Latent Heritable Confounder Mendelian randomization (LHC-MR), Causal Analysis using Summary Effect estimates (CAUSE), and conventional MR approaches assessed bidirectional causation.

Results

LDSC observed only weak genetic correlation (rg = 0.06, P-Value = 0.619) between depression and periodontitis. Analysis of local genetic correlation using ρ-HESS did not reveal loci of significant local genetic covariance. LHC-MR, CAUSE and conventional MR models provided no support for bidirectional causation between depression and periodontitis, with odds ratios ranging from 1.00 to 1.06 in either direction.

Conclusions

Results do not support shared heritability or a causal connection between depression and periodontitis.
Autoimmune Atrophic Gastritis: The Role of miRNA in Relation to Helicobacter Pylori Infection
Authors: Zingone Fabiana , Pilotto Valentina , Cardin Romilda , Maddalo Gemma , Orlando Costanza , Fassan Matteo , Marsilio Ilaria , Collesei Eugenio , Pelizzaro Filippo , Farinati Fabio
Published: July 2022

Introduction

MicroRNAs (miRNAs) have been proposed as diagnostic markers, biomarkers of neoplastic progression, and possible therapeutic targets in several immune-mediated diseases. We aimed to analyze the expression profile of selected miRNAs (miR21, miR142, miR223, miR155) in patients with autoimmune atrophic gastritis (AAG), patients with non-autoimmune multifocal atrophic gastritis (MAG), and healthy control subjects (HC).

Materials and methods

A total of 103 patients with AAG were consecutively recruited for this study among those attending our gastroenterology outpatient clinic. Participating patients were divided into two groups: primary, not Helicobacter pylori (HP)-associated related AAG (n=57, P-AAG) and HP-associated AAG (n=46, HP-AAG); this subgroup included HP-positive patients, patients with previously reported HP infection, and patients harboring antral atrophy, considered as a stigma of HP infection. We also included 20 sex-age-matched MAG patients and 10 HC. Upper endoscopy with gastric biopsies were performed on each AAG and MAG patient. Circulating levels of miR21-5p, miR142-3p, miR223-3p, and miR155-5p were measured by RT-PCR in all groups.

Results

MiR-21 was over-expressed in P-AAG (p=0.02), HP-AAG (p = 0.04), and MAG (p=0.03) compared with HC. By contrast, miR-142 was more expressed in HC than in HP-AAG (p=0.04) and MAG (p=0.03). MiR-155 showed no significant differences among the four subgroups, while, unexpectedly, miR-223 was overexpressed in HC compared to P-AAG (p=0.01), HP-AAG (p=0.003), and MAG (p<0.001), and was higher in P-AAG than in MAG (p=0.05).

Conclusions

MiR-21 was over-expressed in patients with gastric precancerous conditions irrespective of etiology, while in the same subgroups miR-142 and miR-223 were under-expressed compared to healthy controls. Controlling miRNAs up- or downregulation could lead to a breakthrough in treating chronic autoimmune diseases and potentially interfere with the progression to cancer.
Hemidesmosomal Reactivity and Treatment Recommendations in Immune Checkpoint Inhibitor-Induced Bullous Pemphigoid—A Retrospective, Monocentric Study
Authors: Schauer Franziska , Rafei-Shamsabadi David , Mai Shoko , Mai Yosuke , Izumi Kentaro , Meiss Frank , Kiritsi Dimitra
Published: July 2022

Immune checkpoint inhibitors (ICI) induce T-cell-mediated antitumour responses. While ICI were initially successfully applied in metastasized melanoma, they are now approved for several tumour entities. Numerous autoimmune disorders have been reported to occur as adverse events of the treatment, among them bullous pemphigoid (BP), with less than 1% of the patients experiencing ICI-induced BP. This number is higher than the estimated prevalence of autoimmune bullous diseases in the general population of Germany, which lies around 0.05%. We here describe our cohort of eight patients, who developed a bullous pemphigoid under or shortly after ICI treatment. Half of them had a severe subtype (as shown by BPDAI >57) and showed a median onset of ICI-BP after 10 months of ICI initiation. Six patients had a palmar and/or plantar involvement, while oral involvement occurred in one case. All patients had linear epidermal IgG depositions in split skin in the indirect immunofluorescence. In four out of five biopsies available for direct immunofluorescence, linear IgG and C3 depositions were detected at the basement membrane, while one patient showed linear IgM staining. Moderate to high levels of FLBP180 autoantibodies were found in seven of eight cases. The disease can still be active after ICI discontinuation, while rituximab might be required for remission. Finally, four tumour samples were stained histochemically for collagen XVII (BP180), but no enhanced expression was found.
Microplastic prevalence in marine fish from onshore Beibu Gulf, South China Sea
Authors: Koongolla Jayamini Bimali , Lin Lang , Yang Chang-Ping , Pan Yun-Feng , Li Heng-Xiang , Liu Shan , Xu Xiang-Rong
Published: July 2022

In recent years, microplastics have been widely detected in marine fish and may pose potential risks to fish and human health. Even though microplastic pollution is a critical issue, it represents as yet non-quantified threat for some context in the marine environment. In this study, 271 individuals comprising 32 species of marine fish collected from Beibu Gulf were examined for microplastic prevalence, with an aim to provide data on the abundance, physical classification, and chemical characterization of microplastics in the great bay along South China Sea. The results showed that the occurrence rate of microplastics was 93.7%, and the average abundance of microplastics was 1.02 ± 0.18 items per individual (ranging from 0.03 to 4.00 items per individual). Microplastic accumulation was observed with a great variation in different species, body sizes, living habitats, and feeding habits of marine fishes. The dominant polymers identified by μ-FTIR were polyethylene terephthalate and polypropylene. Fibers, smaller sizes (<1 mm), and transparent color were the major characteristics of the detected microplastics, which might be important factors affecting the bioaccumulation. The present study revealed that microplastics in marine fish is a widespread issue in onshore Beibu Gulf.
Effects of Cd addition on soil phosphorus mineralization in reclaimed coastal wetlands along a 100-year reclamation chronosequence in the Pearl River Estuary (China)
Authors: Liu Zhe , Gao Zhaoqin , Bai Junhong , Zhang Guangliang , Tang Ruoxuan , Chen Guozhu
Published: July 2022

The relationship between Cd and soil phosphatase activity has been given some concerns due to serious soil Cd contamination. However, the effects of high-risk Cd pollution on the soil phosphorus mineralization process are still kept unclear in reclaimed coastal wetlands. Here, we investigated the impacts of Cd additions at three levels on phosphorus mineralization and phosphatase activities in reclaimed coastal wetland soils with different reclamation ages (e.g., 100-year, 40-year, and 10-year) in the Pearl River Estuary by a 40-day laboratory incubation experiment. The results showed that lower soil phosphatase activity was observed in the reclaimed wetlands with longer reclamation age, which led to the lower increase in cumulative net phosphorus mineralization and Occluded P. High Cd exposure facilitated the phosphatase activities and phosphorus mineralization through the promotion of Occluded P and Al/Fe-P contents in the 100-year and 10-year reclaimed wetland soils, respectively. The addition of the low-dose of Cd to the soil inhibited phosphorus mineralization in the middle of incubation in 40-year reclaimed wetland soils, while the high-dose Cd addition had little effect. The findings of this work indicate that the older reclaimed wetlands might have lower phosphorus mineralization potentials, while higher Cd pollution might lead to soil phosphorus loss by improving phosphorus mineralization in reclaimed coastal wetlands.
Mitochondria-Related Ferroptosis Drives Cognitive Deficits in Neonatal Mice Following Sevoflurane Administration
Authors: Zhang Piao , Chen Yeru , Zhang ShuXia , Chen Gang
Published: July 2022

Multiple sevoflurane exposure may result in cognitive deficits in neonatal animals. This study attempted to investigate the potential mechanism of sevoflurane-induced neurotoxicity in developing hippocampus. Neonatal animals received sevoflurane anesthesia, then the behavioral tests and Golgi-Cox staining were employed to detect the effect of sevoflurane inhalation in adult mice. And the mitochondrial function was evaluated using MitoSOX staining, Fluo calcium indicators, mitochondrial permeability transition pore (mPTP) assay, and JC-1 probe after sevoflurane administration. Meanwhile, mitochondrial lipid hydroperoxide and ferroptosis were measured by MitoPeDPP and Mito-FerroGreen signals following sevoflurane exposure. Moreover, the ferroptosis and behavioral performance were assessed after deferiprone (DFP) treatment. The results showed that sevoflurane administration induced cognitive impairment accompanied by reducing dendritic length, density, and nodes. Additionally, sevoflurane exposure elevated mitochondrial ROS production and cytoplasm calcium levels, triggered the opening of mPTP, and decreased the mitochondrial membrane potential (MMP). However, supplement of elamipretide (SS-31) effectively reversed mitochondrial dysfunction. Mitochondrial lipid hydroperoxide production was increased after sevoflurane administration, whereas Fer-1 treatment reduced lipid hydroperoxide formation. Sevoflurane exposure induced mitochondrial iron overload, whereas Mito-Tempo treatment reduced iron accumulation. Prussian blue staining showed that the hippocampal iron deposition was apparently increased after sevoflurane inhalation. Additionally, the ferroptosis-related protein expression (including ACSL4, COX2, GPX4, and FTH1) was significantly changed, whereas DFP effectively suppressed ferroptosis and enhanced sevoflurane-induced behavioral malfunction. These findings demonstrated that sevoflurane administration elicited mitochondrial dysfunction and iron dyshomeostasis and eventually resulted in cognitive impairments, whereas protecting mitochondrial function and chelating neurotoxic iron effectively reversed these pathological processes.
Neurological Manifestations of SARS-CoV-2 Infection: Protocol for a Sub-analysis of the COVID-19 Critical Care Consortium Observational Study
Authors: Battaglini Denise , Premraj Lavienraj , Griffee Matthew , Huth Samuel , Fanning Jonathon , Whitman Glenn , Bastos Porto Diego , Arora Rakesh , Durham Lucian , Gnall Eric , Amato Marcelo , Williams Virginie , Noel Alexandre , De Franca Sabrina Araujo , Samoukovic Gordan , Pujo Bambang , Kent David , Marwali Eva , Al-Fares Abdulrahman , Stecher Stephanie-Susanne , Panigada Mauro , Giani Marco , Foti Giuseppe , Pelosi Paolo , Pesenti Antonio , White Nicole Marie , Li Bassi Gianluigi , Suen Jacky , Fraser John F. , Robba Chiara , Cho Sung-Min
Published: July 2022

Introduction

Neurological manifestations and complications in coronavirus disease-2019 (COVID-19) patients are frequent. Prior studies suggested a possible association between neurological complications and fatal outcome, as well as the existence of potential modifiable risk factors associated to their occurrence. Therefore, more information is needed regarding the incidence and type of neurological complications, risk factors, and associated outcomes in COVID-19.

Methods

This is a pre-planned secondary analysis of the international multicenter observational study of the COVID-19 Critical Care Consortium (which collected data both retrospectively and prospectively from the beginning of COVID-19 pandemic) with the aim to describe neurological complications in critically ill COVID-19 patients and to assess the associated risk factors, and outcomes. Adult patients with confirmed COVID-19, admitted to Intensive Care Unit (ICU) will be considered for this analysis. Data collected in the COVID-19 Critical Care Consortium study includes patients' pre-admission characteristics, comorbidities, severity status, and type and severity of neurological complications. In-hospital mortality and neurological outcome were collected at discharge from ICU, and at 28-days.

Ethics and Dissemination

The COVID-19 Critical Care Consortium main study and its amendments have been approved by the Regional Ethics Committee of participating sites. No further approval is required for this secondary analysis.

Trial Registration Number

ACTRN12620000421932.
Microbiome analysis reveals microecological advantages of emerging ditchless rice-crayfish co-culture mode
Authors: Huang Xiaoli , Li Minghao , Huang Ya , Yang Hai , Geng Yi , Ouyang Ping , Chen Defang , Yin Lizi , Yang Shiyong , Jiang Jun , Luo Wei , He Zhi
Published: July 2022

Ditchless rice-crayfish co-culture is an emerging model of rice-crayfish farming that circumvents the potential hazards of digging ditches in traditional rice-crayfish farming. However, due to the complex interactions among crayfish, ambient microbiota, and environmental variables, it is necessary to assess the differences in bacterial structure between ditchless and traditional rice-crayfish culture. In this study, the crayfish culture area in the Sichuan basin was selected as the study area, and the bacterial communities of two rice-crayfish culture systems were compared by high-throughput sequencing of 16S rDNA. The results showed that the ditchless system had lower water depth, higher dissolved oxygen, lower total ammonia nitrogen and lower morbidity. There are intuitive differences in the composition of environmental bacterial communities due to environmental changes, even if they are similar in composition at the phylum level. Microbiota in sediments from ditchless systems appear to produce less ammonia nitrogen. The abundance of the pathogens colonizing the intestine of ditchless crayfish was lower than ditched one, and the composition was similar to water. Ditch-farmed crayfish appear to be more susceptible to environmental microbes and have a more fragile intestinal structure. Water depth and dissolved oxygen are the main environmental factors that determine the distribution of microbiota. This study is the first to investigate the bacterial ecology of a ditchless rice- crayfish farming system. The results show that the ditchless rice-crayfish culture model has a more superior bacterial system than the traditional rice-crayfish culture.