bluuebunny/crossref_metadata_2025 huggingface dataset:
The high energy X-ray probe (HEX-P): constraining supermassive black hole growth with population spin measurements
Authors: Piotrowska J. M. , García J. A. , Walton D. J. , Beckmann R. S. , Stern D. , Ballantyne D. R. , Wilkins D. R. , Bianchi S. , Boorman P. G. , Buchner J. , Chen C.-T. , Coppi P. , Dauser T. , Fabian A. C. , Kammoun E. , Madsen K. , Mallick L. , Matt G. , Matzeu G. , Nardini E. , Pizzetti A. , Puccetti S. , Ricci C. , Tombesi F. , Torres-Albà N. , Wong K.-W.
Published: March 2024

Constraining the primary growth channel of supermassive black holes (SMBHs) remains one the most actively debated questions in the context of cosmological structure formation. Owing to the expected connection between SMBH spin parameter evolution and the accretion and merger history of individual black holes, population spin measurements offer a rare observational window into the cosmic growth of SMBHs. As of today, the most common method for estimating SMBH spin relies on modeling the relativistically broaden atomic profiles in the reflection spectrum observed in X-rays. In this paper, we study the observational requirements needed to confidently distinguish between the primary SMBH growth channels based on their distinct spin-mass distributions predicted by the Horizon-AGN cosmological simulation. Indoing so, we characterize outstanding limitations associated with the existing measurements and discuss the landscape of future observational campaigns which could be planned and executed with future X-ray observatories. We focus our attention on the High-Energy X-ray Probe (HEX-P), a proposed probe-class mission designed to serve the high-energy community in the 2030s.
Physical exercise and cognition in older adults, a scientific approach scanty reported in Latin America and Caribbean populations
Authors: Jiménez-Maldonado Alberto , Rentería Iván , Johnson David K. , Moncada-Jiménez José , García-Suárez Patricia C.
Published: March 2024

The advancement of public services, including the increased accessibility of health services, has led to a rise in life expectancy globally. As a result, aging populations are becoming more prevalent, raising concerns about cognitive decline. Fortunately, non-pharmacological methods, such as physical exercise, have been shown to mitigate the effects of aging on the brain. In this perspective article, we examined meta-analyses on the impact of physical exercise on cognition in older adults. The results indicate that combined exercise (i.e., aerobic plus strength training), has a significant positive effect on overall cognition and executive function. However, we found a lack of scientific studies on this topic in Latin American and Caribbean countries. Therefore, there is a pressing need for research to identify the feasibility of physical exercise interventions to improve cognitive skills in older adults from these regions.
Comparison of uncut Roux-en-Y anastomosis and Billroth-II with Braun anastomosis after distal gastrectomy
Authors: Wei Tianxiao , Wu Zhouqiao , Chen Yufan , Li Yingai , Pang Fei , Shan Fei , Li Ziyu , Ji Jiafu
Published: March 2024

Background

This study aimed to compare the clinical outcomes and patient benefits of uncut Roux-en-Y (URY) anastomosis and Billroth-II with Braun (BB) anastomosis after distal gastrectomy.

Methods

We retrospectively reviewed the data of patients who underwent URY or BB anastomosis after distal gastrectomy between March 2015 and December 2017. Clinical characteristics, survival data, postoperative recovery data, and long-term outcomes were recorded and compared between the two groups.

Results

A total of 231 patients were included, with 167 in the URY group and 64 in the BB group. Kaplan–Meier curves for overall survival showed no differences after propensity score matching (p = 0.488). Long-term postoperative quality of life evaluation also showed no significant differences. Compared to the BB group, patients in the URY group had a significantly shorter time to start a liquid diet after propensity score matching (67.6 h vs. 46.5 h, p = 0.003), and a lower occurrence of bile reflux on follow-up gastroscopy (p < 0.001).

Conclusion

The URY anastomosis appears to be a feasible method for digestive tract reconstruction after distal gastrectomy, resulting in less bile reflux and better postoperative recovery. However, there is no significant difference between URY and BB anastomosis in terms of overall survival and long-term quality of life.
Serological prevalence of the Schmallenberg virus in domestic and wild hosts worldwide: a systematic review and meta-analysis
Authors: Dagnaw Melkie , Solomon Atsede , Dagnew Binyam
Published: March 2024

Schmallenberg virus (SBV) is an arthropod-borne virus that emerged recently in northwestern Europe in 2011 that affects domestic and wild ruminants and induces abortion, stillbirth, and newborns with congenital anomalies. Since its discovery, SBV has spread very rapidly to too many countries in the world. The overall serological investigation of SBV is needed to improve modeling predictions and assess the overall impact on ruminant animals, which helps to design interventions for control and prevention strategies. Thus, this study aimed to estimate the overall serological assay of SBV in both domestic and wild ruminants around the world. This systematic review was conducted as per the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. International databases were employed To search for relevant articles. The pooled prevalence with a 95% confidence interval was calculated with a random effects model. The Cochran’s Q test, τ2, and I2 were used to assess the sources of heterogeneity. In the current meta-analysis, a total of 41 articles were included. The overall pooled proportion of SBV in domestic and wild ruminants was 49 and 26%, respectively. Substantial heterogeneity was observed in studies on domestic ruminants (I2 = 99.7%; p < 0.01) and studies on wild ruminants (I2 = 97.9%; p < 0.01). The pooled prevalence of SBV was significantly associated with publication time, detection techniques, and species of animals. According to the subgroup analysis, the highest pooled prevalence of SBV was reported in cattle (59%), followed by sheep (37%) and goat (18%). In addition to the subgroup analysis based on publication year, the pooled prevalence of SBV infection has become endemic since 2013 (49%) among domestic animals in the world. Of the diagnostic tests used, the highest anti-SBV antibodies (66%) were detected by a virus neutralization test. In this meta-analysis, the major wild animals that were infected by SBV were red deer, roe deer, fallow deer, mouflon, and wild boar. The highest sub-pooled prevalence of SBV was found in roe deer (46%), followed by fallow deer (30%), red deer (27%), mouflon (22%), and wild boar (11%). In general, the prevalence of SBV was high in cattle among domestic ruminants and in roe deer among wild animals. According to the current information provided by this meta-analysis, evidence-based risk management measures should be established to restrict SBV spread in both domestic and wild ruminants.
Tobramycin Systemic Absorption in Lung Transplant Recipients Treated With Inhaled Tobramycin: A Cohort Study
Authors: Sempere Abiu , Los-Arcos Ibai , Sacanell Judith , Berastegui Cristina , Campany-Herrero David , Vima Jaume , Martín-Gómez María Teresa , Sánchez Leire , Martínez-González Daniel , Bravo Carles , Len Oscar , Gavaldà Joan
Published: March 2024

Inhaled tobramycin treatment has been associated with nephrotoxicity in some case reports, but limited data are available about serum levels and its possible systemic absorption in lung transplant recipients (LTR). We conducted a single-center, observational and retrospective study of all adult (>18 years old) LTR treated with inhaled tobramycin for at least 3 days between June 2019 and February 2022. Trough serum levels were collected and >2 μg/mL was considered a high drug level. The primary outcome assessed the presence of detectable trough levels, while the secondary outcome focused on the occurrence of acute kidney injury (AKI) in individuals with detectable trough levels. Thirty-four patients, with a median age of 60 years, were enrolled. The primary indications for treatment were donor bronchial aspirate bacterial isolation (18 patients) and tracheobronchitis (15 patients). In total, 28 patients (82%) exhibited detectable serum levels, with 9 (26%) presenting high levels (>2 μg/mL). Furthermore, 9 patients (26%) developed acute kidney injury during the treatment course. Median trough tobramycin levels were significantly elevated in invasively mechanically ventilated patients compared to non-ventilated individuals (2.5 μg/mL vs. 0.48 μg/mL) (p < 0.001). Inhaled tobramycin administration in LTRs, particularly in those requiring invasive mechanical ventilation, may result in substantial systemic absorption.
Payment Liquidity in the Light of Changes in the Central Bank Toolkit
Authors: Kollár Adrián
Published: March 2024

This study examines the impact of the changes implemented by the Magyar Nemzeti Bank in the central bank toolkit on the liquidity of payment system1 participants over the period between 2020 and the end of 2023 H1. In response to the economic challenges of recent years, the central bank toolkit changed significantly during the period under review. While the economic and financial crisis stemming from the Covid-19 pandemic required interbank liquidity expansion measures, in 2022 steps to absorb excess liquidity were necessary to fine-tune monetary transmission and achieve and maintain price stability. The analysis focuses on the impact of specific toolkit adjustments on the liquidity of the payment system, while also demonstrating why interbank liquidity is separated from payment system liquidity, and which toolkit modifications have typically had a significant impact on the liquidity of the payment system.
Challenges in Reducing Bias Using Post-Processing Fairness for Breast Cancer Stage Classification with Deep Learning
Authors: Soltan Armin , Washington Peter
Published: March 2024

Breast cancer is the most common cancer affecting women globally. Despite the significant impact of deep learning models on breast cancer diagnosis and treatment, achieving fairness or equitable outcomes across diverse populations remains a challenge when some demographic groups are underrepresented in the training data. We quantified the bias of models trained to predict breast cancer stage from a dataset consisting of 1000 biopsies from 842 patients provided by AIM-Ahead (Artificial Intelligence/Machine Learning Consortium to Advance Health Equity and Researcher Diversity). Notably, the majority of data (over 70%) were from White patients. We found that prior to post-processing adjustments, all deep learning models we trained consistently performed better for White patients than for non-White patients. After model calibration, we observed mixed results, with only some models demonstrating improved performance. This work provides a case study of bias in breast cancer medical imaging models and highlights the challenges in using post-processing to attempt to achieve fairness.
SMA Wire Use in Hybrid Twisting and Bending/Extending Soft Fiber-Reinforced Actuators
Authors: Kashef Tabrizian Seyedreza , Cedric Fovel , Terryn Seppe , Vanderborght Bram
Published: March 2024

Soft fiber-reinforced actuators have demonstrated significant potential across various robotics applications. However, the actuation motion in these actuators is typically limited to a single type of motion behavior, such as bending, extending, and twisting. Additionally, a combination of bending with twisting and extending with twisting can occur in fiber-reinforced actuators. This paper presents two novel hybrid actuators in which shape memory alloy (SMA) wires are used as reinforcement for pneumatic actuation, and upon electrical activation, they create a twisting motion. As a result, the hybrid soft SMA-reinforced actuators can select between twisting and bending, as well as twisting and extending. In pneumatic mode, a bending angle of 40° and a longitudinal strain of 20% were achieved for the bending/twisting and extending/twisting actuators, respectively. When the SMA wires are electrically activated by the Joule effect, the actuators achieved more than 90% of the maximum twisting angle (24°) in almost 2 s. Passive recovery, facilitated by the elastic response of the soft chamber, took approximately 10 s. The double-helical reinforcement by SMA wires not only enables twisting in both directions but also serves as an active recovery mechanism to more rapidly return the finger to the initial position (within 2 s). The resulting pneumatic–electric-driven soft actuators enhance dexterity and versatility, making them suitable for applications in walking robots, in-pipe crawling robots, and in-hand manipulation.
Nutritional, Utility, and Sensory Quality and Safety of Sunflower Oil on the Central European Market
Authors: Nakonechna Kristina , Ilko Vojtech , Berčíková Markéta , Vietoris Vladimír , Panovská Zdeňka , Doležal Marek
Published: March 2024

In the quality monitoring of 18 sunflower oil samples from the EU market, 14 were refined and 4 were cold-pressed. They demonstrated high quality of technological processing with low values of trans-unsaturated fatty acids, acid value, and peroxide value and also met the limits set by legislation in the content of process contaminants 3-monochloropropane-1,2-diol (3-MCPD) esters and glycidyl esters. Measurements of oxidative stability showed a difference in utility value. The average induction period of the oils from the traditional varieties was 2.6 h, predisposing them to cold cooking or short-term frying, while the 11.8 h of the four high oleic sunflower oils (HOSO) indicates the possibility of long-term heat stress. The nutritional benefit is the average vitamin E content of 663 mg/kg oil. The overall sensory quality of the samples was evaluated by a 12-member panel of trained assessors. On the seven-point category scale, the oils were of good to exceptional quality. The cold-pressed oils (CPOs) differed in having, on average, lower trans-unsaturated fatty acid content, process contaminants at unmeasurable levels, and, on average, higher vitamin E concentrations. The specific organoleptic properties of the CPOs were characterized by a pleasant nutty and sunflower seed flavor.
The Molecular Mechanism of circRNA-11228/miR-103/INSIG1 Pathway Regulating Milk Fat Synthesis in Bovine Mammary Epithelial Cells
Authors: Li Xiaofen , Wu Yanni , Wang Yuhao , Yang Xiaozhi , Gao Rui , Lu Qinyue , Lv Xiaoyang , Chen Zhi
Published: March 2024

Milk, known for its high content of short- and medium-chain fatty acids and unsaturated fatty acids, has attracted substantial attention due to its nutritional and health value. The regulation of fatty acid metabolism by non-coding RNAs has become a subject of growing attention, particularly in relation to fatty acid production at the transcriptional/epigenetic and post-transcriptional levels. This study established the circRNA-11228/miR-103/INSIG1 (insulin-inducible gene) regulatory network using methods such as qRT-PCR, dual luciferase reporting, and Western blot, with INSIG1 serving as the starting point. The experimental validation of circRNA-11228’s impact on cholesterol levels, lipid droplet secretion, and unsaturated fatty acid content was conducted using various assays, including triglycerides, cholesterol, oil red O, andEdU(5-ethynyl-2’-deoxyuridine) in bovine mammary epithelial cells (BMECs). Furthermore, the transfection of mimics and inhibitors synthesized from miR-103 into BMECs confirmed that miR-103 can promote cholesterol synthesis and lipid droplet secretion. Conversely, the INSIG1 gene was found to inhibit cholesterol synthesis and lipid droplet secretion. The “remediation” experiment validated the ability of miR-103 to alleviate the cellular effect of circRNA-11228. Taken together, our findings indicate that the binding of circRNA-11228 to miR-103 inhibits the expression of the target gene INSIG, thereby regulating milk fat production in BMECs. This study offers novel insights into producing high-quality milk and new ways to improve the dietary composition of residents.
NRX-101 (D-Cycloserine + Lurasidone) Is Active against Drug-Resistant Urinary Pathogens In Vitro
Authors: Sapko Michael T. , Manyak Michael , Panicucci Riccardo , Javitt Jonathan C.
Published: March 2024

D-Cycloserine (DCS) is a broad-spectrum antibiotic that is currently FDA-approved to treat tuberculosis (TB) disease and urinary tract infection (UTI). Despite numerous reports showing good clinical efficacy, DCS fell out of favor as a UTI treatment because of its propensity to cause side effects. NRX-101, a fixed-dose combination of DCS and lurasidone, has been awarded Qualified Infectious Disease Product and Fast Track Designation by the FDA. In this study, we tested NRX-101 against the urinary tract pathogens Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter baumannii in cation-adjusted Mueller–Hinton broth (caMHB) and artificial urine media (AUM). Several strains were multidrug resistant. Test compounds were serially diluted in broth/media. Minimum inhibitory concentration (MIC) was defined as the lowest concentration of the test compound at which no bacterial growth was observed. DCS exhibited antibacterial efficacy against all strains tested while lurasidone did not appreciably affect the antibacterial action of DCS in vitro. In AUM, the MICs ranged from 128 to 512 mcg/mL for both DCS and NRX-101. In caMHB, MICs ranged from 8 to 1024 mcg/mL for NRX-101 and 32 to 512 mcg/mL for DCS alone. Our data confirm that DCS has antibacterial activity against reference and drug-resistant urinary pathogens. Furthermore, lurasidone does not interfere with DCS’s antimicrobial action in vitro. These results support the clinical development of NRX-101 as a treatment for complicated urinary tract infections.
Impact of Interfering Substances on the Bactericidal Efficacy of Different Commercially Available Hypochlorous Acid-Based Wound Irrigation Solutions Commonly Found in South-East Asia
Authors: Yap Jiann Wen , Ismail Neni Iffanida , Lee Cheng Shoou , Oh Ding Yuan
Published: March 2024

The high prevalence of chronic wounds is a growing concern. Recently, hypochlorous acid (HOCl)-based solutions were introduced as an alternative antimicrobial for wound cleansing. In this study, we assessed the in vitro bactericidal activities of seven commercially available wound irrigation products commonly found in South-East Asia. The evaluation was conducted using quantitative suspension method, EN 13727 in either low or high protein conditions. Under low protein conditions, four out of the five HOCl products achieved bactericidal activity (≥5 log10 reduction factor; RF) within 2–5 min, and only one product achieved 5 log10 RF at 15 s. None of the HOCl achieved 5 log10 RF under high protein, even after 30 min of exposure time. In contrast, protein interference on the antimicrobial activities of polyhexamethylene biguanide-based product is less pronounced (low protein: 60 s vs. high protein: 2 min to attain ≥5 log10 RF). Octenidine dihydrochloride is the only active not affected by protein interference achieving ≥5 log10 RF within 15 s in both low and high protein conditions. These findings warrant the need to screen antimicrobial wound care products, especially HOCl-based products, in high protein condition to better reflect the antimicrobial activities in wound care.
Artemisinin May Disrupt Hyphae Formation by Suppressing Biofilm-Related Genes of Candida albicans: In Vitro and In Silico Approaches
Authors: Sumlu Esra , Aydin Merve , Korucu Emine Nedime , Alyar Saliha , Nsangou Ahmed Moustapha
Published: March 2024

This study aimed to assess the antifungal and antibiofilm efficacy of artemisinin against Candida (C.) species, analyze its impact on gene expression levels within C. albicans biofilms, and investigate the molecular interactions through molecular docking. The antifungal efficacy of artemisinin on a variety of Candida species, including fluconazole-resistant and -susceptible species, was evaluated by the microdilution method. The effect of artemisinin on C. albicans biofilm formation was investigated by MTT and FESEM. The mRNA expression of the genes related to biofilm was analyzed by qRT-PCR. In addition, molecular docking analysis was used to understand the interaction between artemisinin and C. albicans at the molecular level with RAS1-cAMP-EFG1 and EFG1-regulated genes. Artemisinin showed higher sensitivity against non-albicans Candida strains. Furthermore, artemisinin was strongly inhibitory against C. albicans biofilms at 640 µg/mL. Artemisinin downregulated adhesion-related genes ALS3, HWP1, and ECE1, hyphal development genes UME6 and HGC1, and hyphal CAMP-dependent protein kinase regulators CYR1, RAS1, and EFG1. Furthermore, molecular docking analysis revealed that artemisinin and EFG1 had the highest affinity, followed by UME6. FESEM analysis showed that the fluconazole- and artemisinin-treated groups exhibited a reduced hyphal network, unusual surface bulges, and the formation of pores on the cell surfaces. Our study suggests that artemisinin may have antifungal potential and showed a remarkable antibiofilm activity by significantly suppressing adhesion and hyphal development through interaction with key proteins involved in biofilm formation, such as EFG1.
Obesogenic Diet in Mice Leads to Inflammation and Oxidative Stress in the Mother in Association with Sex-Specific Changes in Fetal Development, Inflammatory Markers and Placental Transcriptome
Authors: Candia Alejandro A. , Lean Samantha C. , Zhang Cindy X. W. , McKeating Daniel R. , Cochrane Anna , Gulacsi Edina , Herrera Emilio A. , Krause Bernardo J. , Sferruzzi-Perri Amanda N.
Published: March 2024

Background: Obesity during pregnancy is related to adverse maternal and neonatal outcomes. Factors involved in these outcomes may include increased maternal insulin resistance, inflammation, oxidative stress, and nutrient mishandling. The placenta is the primary determinant of fetal outcomes, and its function can be impacted by maternal obesity. The aim of this study on mice was to determine the effect of obesity on maternal lipid handling, inflammatory and redox state, and placental oxidative stress, inflammatory signaling, and gene expression relative to female and male fetal growth. Methods: Female mice were fed control or obesogenic high-fat/high-sugar diet (HFHS) from 9 weeks prior to, and during, pregnancy. On day 18.5 of pregnancy, maternal plasma, and liver, placenta, and fetal serum were collected to examine the immune and redox states. The placental labyrinth zone (Lz) was dissected for RNA-sequencing analysis of gene expression changes. Results: the HFHS diet induced, in the dams, hepatic steatosis, oxidative stress (reduced catalase, elevated protein oxidation) and the activation of pro-inflammatory pathways (p38-MAPK), along with imbalanced circulating cytokine concentrations (increased IL-6 and decreased IL-5 and IL-17A). HFHS fetuses were asymmetrically growth-restricted, showing sex-specific changes in circulating cytokines (GM-CSF, TNF-α, IL-6 and IFN-γ). The morphology of the placenta Lz was modified by an HFHS diet, in association with sex-specific alterations in the expression of genes and proteins implicated in oxidative stress, inflammation, and stress signaling. Placental gene expression changes were comparable to that seen in models of intrauterine inflammation and were related to a transcriptional network involving transcription factors, LYL1 and PLAG1. Conclusion: This study shows that fetal growth restriction with maternal obesity is related to elevated oxidative stress, inflammatory pathways, and sex-specific placental changes. Our data are important, given the marked consequences and the rising rates of obesity worldwide.
Effects of Grape Pomace on Growth Performance, Nitrogen Metabolism, Antioxidants, and Microbial Diversity in Angus Bulls
Authors: Li Yingqi , Shi Changxiao , Deng Jiajie , Qiu Xinjun , Zhang Siyu , Wang Huili , Qin Xiaoli , He Yang , Cao Binghai , Su Huawei
Published: March 2024

Polyphenol-rich grape pomace (GP) represents a valuable processing by-product with considerable potential as sustainable livestock feed. This study aimed to investigate the effects of different levels of GP on the growth performance and nitrogen utilization efficiency, antioxidant activity, and rumen and rectum microbiota of Angus bulls. Thirty Angus bulls were allocated three dietary treatments according to a completely randomized design: 0% (G0), 10% (G10), and 20% (G20) corn silage dry matter replaced with dried GP dry matter. The results showed that the average daily gain (ADG) of the G0 group and G10 group was higher than that of the G20 group (p < 0.05); urinary nitrogen levels decreased linearly with the addition of GP (linear, p < 0.05). In terms of antioxidants, the levels of catalase (CAT) in the G10 group were higher than in the G0 and G20 groups (p < 0.05), and the total antioxidative capacity (T-AOC) was significantly higher than that in the G20 group (p < 0.05). In addition, in the analysis of a microbial network diagram, the G10 group had better microbial community complexity and stability. Overall, these findings offer valuable insights into the potential benefits of incorporating GP into the diet of ruminants.
The Role of the Myokine Irisin in the Protection and Carcinogenesis of the Gastrointestinal Tract
Authors: Pinkas Monika , Brzozowski Tomasz
Published: March 2024

Recently discovered irisin, a member of the myokines family, is a potential mediator of exercise-induced energy metabolism and a factor promoting browning of the white adipose tissue. Recent evidence indicates that this myokine, released from contracting muscles, can mediate the beneficial effects of exercise on health. Irisin may be a potential therapeutic agent against obesity and has been shown to play an important role in the protection of various cells, tissues, and organs due to its anti-inflammatory, antioxidative, and anti-cancer properties. Our aim was to review the recent experimental and clinical studies on irisin and its expression, release into the bloodstream, tissue targets, and potential contribution to the protective effects of exercise in the gastrointestinal tract. Particular emphasis was placed on inflammatory bowel disease, intestinal ischemia/reperfusion injury, periodontitis, and other digestive tract disorders, including carcinogenesis. Overall, irisin holds significant potential as a novel target molecule, offering a safe and therapeutic approach to treating various gastrointestinal diseases.
Effect of Different Control Strategies on the Heat Transfer Mechanism of Helical Energy Piles
Authors: Wei Pan , Li Kongqing , Yu Chenfeng , Han Qiaoyun
Published: March 2024

In this paper, numerical simulations of a special energy pile, which constitutes a spiral-injected pipe and one straight discharge pile for Geothermal Heat Pump Systems (SGHEs-P(parallel)), were conducted by Fluent software. The effects of the spiral pitches on the heat transfer rate based on the G-function method and peripheral soil temperature of the pile were investigated under continuous and intermittent operation strategies. The impact of spiral tube sizing on the surface heat transfer coefficients was studied. The results indicated that SGHEs-P may be preferred for office buildings under intermittent operation conditions. For a short period, the temperature profiles and heat transfer efficiency of SGHEs-P were mainly influenced by the fluid type, length of the spiral tube, and spiral pitch. The smaller the spiral pitch, the more uniform the temperature distribution, and the better the heat transfer effect, but the heat transfer per unit depth of pile decreased. The average temperature variation curve of the soil around the energy pile with different spiral pitches was simulated and obtained over time. Meanwhile, the impact of spiral radius, spiral pitch, and spiral tube radius on the convective heat transfer coefficient was also presented. Through data fitting, the formulas for the correction coefficients of spiral radius, spiral pitch, and spiral tube radius on convective heat transfer coefficient were obtained, respectively.
Fully Digitalized Workflow of Flipper Fabrication: Different Three-Dimensional Printing Strategies and Characteristics
Authors: Rana Shilpa , Lee Du-Hyeong
Published: March 2024

A flipper is an interim removable partial denture used for immediate esthetic restoration and space maintenance for a limited period before definitive treatment. Traditional methods for fabricating flippers are labor-intensive and manual. With the advent of technological advancement in dentistry, fully digitalized fabrication including computer designing and three-dimensional (3D) printing can be used. This study aimed to demonstrate the complete digital workflow for fabricating flippers and compare the features of different digital methods. In a partially edentulous patient, three flippers were fabricated using different digital protocols: individual printing (tooth and base parts) and combination, i.e., one-body printing and gingival layering and one-body printing and gingival coloring. Each flipper was tried into the oral cavity, and the adaptation of flippers was evaluated using the triple-scan technique. This study confirms that fully digital fabrication of a flipper is an effective method compared with traditional methods and shows various digital workflows available in 3D printing. Thus, an appropriate fabrication method must be selected for each situation.
Effects of a Physical Training Program on Cognitive and Physical Performance and Health-Related Variables in Professional esports Players: A Pilot Study
Authors: Sanz-Matesanz Manuel , Martínez-Aranda Luis Manuel , Gea-García Gemma María
Published: March 2024

The present study focused on the effects of a physical training program on physical- and psychological-related variables in professional esports players. Five professional League of Legends (LOL) players belonging to the highest national category participated in this study. Data were obtained before and after a 5 h virtual training session (acute), as well as before and after an 8-week intervention period, focused on decreasing the impact of fatigue on players’ health and performance. The results showed that a complete virtual training session influences their performance, decreasing jump height and joint mobility (p < 0.01), as well as increasing perceived physical and cognitive exertion by 76.9% (p < 0.01) and 166.67% on the “Rating of Fatigue” scale (p = 0.002). Moreover, the intervention was able to reduce the impact of fatigue (30.8% and 43.3% reduction in cognitive and overall fatigue, respectively, p < 0.05), improving, at the same time, the amount of muscle mass by 2%, jumping ability by 9.8% to 21%, and strength levels in various exercises by 63% to 173% (p < 0.01). The implementation of a physical training program is capable of reducing the players’ fatigue perception, improving their physical conditioning and health status, and decreasing the injury risk.
A Zero-Reference Low-Light Image-Enhancement Approach Based on Noise Estimation
Authors: Cao Pingping , Niu Qiang , Zhu Yanping , Li Tao
Published: March 2024

A novel zero-reference low-light image-enhancement approach based on noise estimation (ZLEN) is proposed to mitigate noise interference in image-enhancement processes, while the tenets of zero-reference and lightweight network architecture are maintained. ZLEN improves the high-order curve expression governing the mapping of low-light images to their enhanced counterparts, addressing image noise through a meticulously designed noise-estimation module and a zero-reference noise loss function. First, the higher-order curve expression with a noise term is defined, and then the noise map undergoes feature extraction through the semantic-aware attention module; following this, the resulting features are integrated with the low-light image. Ultimately, a lightweight convolutional neural network is adjusted to estimate higher-order curve parameters that link the low-light image to its enhanced version. Notably, ZLEN achieves luminance enhancement and noise reduction without paired or unpaired training data. Rigorous qualitative and quantitative evaluations were conducted on diverse benchmark datasets, demonstrating that ZLEN attained state-of-the-art (SOAT) status among existing zero-reference and unpaired-reference image-enhancement methodologies, while it exhibited comparable performance to full-reference image-enhancement methods. To confirm the practicality and robustness of ZLEN, the luminance enhancement was applied to mine images, which yielded satisfactory results.
Determining Steady-State Operation Criteria Using Transient Performance Modelling and Steady-State Diagnostics
Authors: Mathioudakis Konstantinos , Aretakis Nikolaos , Alexiou Alexios
Published: March 2024

Data from the steady-state operation of gas turbine engines are used in gas path diagnostic procedures. A method to identify steady-state operation is thus required. This paper initially explains and demonstrates the factors that cause a deviation in engine health when transient data are used for diagnosis and shows that there is a threshold in the slope of time traces, below which the variation in engine health parameters is acceptable. A methodology for deriving a criterion for steady-state operation based on actual flight data is then presented. The slope of the exhaust gas temperature variation with time and the size of its time-series window, from which this slope is determined, are the required parameters that must be specified when applying this criterion. It is found that the values of these parameters must be selected so that a sufficient number of steady-state points are available without compromising the accuracy of the diagnostic procedure.
Comparative Analysis of Local Differential Privacy Schemes in Healthcare Datasets
Authors: Hernandez-Matamoros Andres , Kikuchi Hiroaki
Published: March 2024

In the rapidly evolving landscape of healthcare technology, the critical need for robust privacy safeguards is undeniable. Local Differential Privacy (LDP) offers a potential solution to address privacy concerns in data-rich industries. However, challenges such as the curse of dimensionality arise when dealing with multidimensional data. This is particularly pronounced in k-way joint probability estimation, where higher values of k lead to decreased accuracy. To overcome these challenges, we propose the integration of Bayesian Ridge Regression (BRR), known for its effectiveness in handling multicollinearity. Our approach demonstrates robustness, manifesting a noteworthy reduction in average variant distance when compared to baseline algorithms such as LOPUB and LOCOP. Additionally, we leverage the R-squared metric to highlight BRR’s advantages, illustrating its performance relative to LASSO, as LOPUB and LOCOP are based on it. This paper addresses a relevant concern related to datasets exhibiting high correlation between attributes, potentially allowing the extraction of information from one attribute to another. We convincingly show the superior performance of BRR over LOPUB and LOCOP across 15 datasets with varying average correlation attributes. Healthcare takes center stage in this collection of datasets. Moreover, the datasets explore diverse fields such as finance, travel, and social science. In summary, our proposed approach consistently outperforms the LOPUB and LOCOP algorithms, particularly when operating under smaller privacy budgets and with datasets characterized by lower average correlation attributes. This signifies the efficacy of Bayesian Ridge Regression in enhancing privacy safeguards in healthcare technology.
New Methods in Digital Wood Anatomy: The Use of Pixel-Contrast Densitometry with Example of Angiosperm Shrubs in Southern Siberia
Authors: Khudykh Timofey A. , Belokopytova Liliana V. , Yang Bao , Kholdaenko Yulia A. , Babushkina Elena A. , Vaganov Eugene A.
Published: March 2024

This methodological study describes the adaptation of a new method in digital wood anatomy, pixel-contrast densitometry, for angiosperm species. The new method was tested on eight species of shrubs and small trees in Southern Siberia, whose wood structure varies from ring-porous to diffuse-porous, with different spatial organizations of vessels. A two-step transformation of wood cross-section photographs by smoothing and Otsu’s classification algorithm was proposed to separate images into cell wall areas and empty spaces within (lumen) and between cells. Good synchronicity between measurements within the ring allowed us to create profiles of wood porosity (proportion of empty spaces) describing the growth ring structure and capturing inter-annual differences between rings. For longer-lived species, 14–32-year series from at least ten specimens were measured. Their analysis revealed that maximum (for all wood types), mean, and minimum porosity (for diffuse-porous wood) in the ring have common external signals, mostly independent of ring width, i.e., they can be used as ecological indicators. Further research directions include a comparison of this method with other approaches in densitometry, clarification of sample processing, and the extraction of ecologically meaningful data from wood structures.
Mitochondrial and Nuclear DNA Variants in Amyotrophic Lateral Sclerosis: Enrichment in the Mitochondrial Control Region and Sirtuin Pathway Genes in Spinal Cord Tissue
Authors: Cox Sharon Natasha , Lo Giudice Claudio , Lavecchia Anna , Poeta Maria Luana , Chiara Matteo , Picardi Ernesto , Pesole Graziano
Published: March 2024

Amyotrophic Lateral Sclerosis (ALS) is a progressive disease with prevalent mitochondrial dysfunctions affecting both upper and lower motor neurons in the motor cortex, brainstem, and spinal cord. Despite mitochondria having their own genome (mtDNA), in humans, most mitochondrial genes are encoded by the nuclear genome (nDNA). Our study aimed to simultaneously screen for nDNA and mtDNA genomes to assess for specific variant enrichment in ALS compared to control tissues. Here, we analysed whole exome (WES) and whole genome (WGS) sequencing data from spinal cord tissues, respectively, of 6 and 12 human donors. A total of 31,257 and 301,241 variants in nuclear-encoded mitochondrial genes were identified from WES and WGS, respectively, while mtDNA reads accounted for 73 and 332 variants. Despite technical differences, both datasets consistently revealed a specific enrichment of variants in the mitochondrial Control Region (CR) and in several of these genes directly associated with mitochondrial dynamics or with Sirtuin pathway genes within ALS tissues. Overall, our data support the hypothesis of a variant burden in specific genes, highlighting potential actionable targets for therapeutic interventions in ALS.
Unraveling the Predictors for Delirium and ICU Stay Duration in Patients with Heart Failure and Reduced Ejection Fraction (HFrEF) Undergoing Coronary Artery Bypass Grafting—A Multicentric Analysis
Authors: Rustenbach Christian Jörg , Reichert Stefan , Berger Rafal , Schano Julia , Nemeth Attila , Haeberle Helene , Charotte Christophe , Caldonazo Tulio , Saqer Ibrahim , Saha Shekhar , Schnackenburg Philipp , Djordjevic Ilija , Krasivskyi Ihor , Wendt Stefanie , Serna-Higuita Lina Maria , Doenst Torsten , Hagl Christian , Wahlers Thorsten , Schlensak Christian , Sandoval Boburg Rodrigo
Published: March 2024

Objective: This study assesses predictors for postoperative delirium (POD) and ICU stay durations in HFrEF patients undergoing CABG, focusing on ONCAB versus OPCAB surgical methods. Summary Background Data: In cardiac surgery, especially CABG, POD significantly impacts patient recovery and healthcare resource utilization. With varying incidences based on surgical techniques, this study provides an in-depth analysis of POD in the context of HFrEF patients, a group particularly susceptible to this complication. Methods: A retrospective analysis of 572 patients who underwent isolated CABG surgery with a preoperative ejection fraction under 40% was conducted at four German university hospitals. Patients were categorized into ONCAB and OPCAB groups for comparative analysis. Results: Age and Euro Score II were significant predictors of POD. The ONCAB group showed higher incidences of re-sternotomy (OR: 3.37), ECLS requirement (OR: 2.29), and AKI (OR: 1.49), whereas OPCAB was associated with a lower incidence of delirium. Statistical analysis indicated a significant difference in ICU stay durations between the two groups, influenced by surgical complexity and postoperative complications. Conclusions: This study underscores the importance of surgical technique in determining postoperative outcomes in HFrEF patients undergoing CABG. OPCAB may offer advantages in reducing POD incidence. These findings suggest the need for tailored surgical decisions and comprehensive care strategies to enhance patient recovery and optimize healthcare resources.
Serum Levels of S100A8/A9 as a Biomarker of Disease Activity in Patients with IgA Vasculitis
Authors: Srsen Sasa , Held Martina , Sestan Mario , Kifer Nastasia , Kozmar Ana , Supe Domic Daniela , Benzon Benjamin , Gagro Alenka , Frkovic Marijan , Jelusic Marija
Published: March 2024

S100A8/A9 protein is a well-known marker of disease activity or severity in many autoimmune and autoinflammatory diseases, but there have not been many studies about the role of S100A8/A9 in IgA vasculitis (IgAV). The aim of our study was to evaluate S100A8/A9 as a possible biomarker of activity in IgAV. We measured the serum levels of S100A8/A9 in pediatric patients with IgA vasculitis at the onset of the disease, after three months, and after six months. We compared these levels between patients with active disease, remission, and a control group, and assessed their correlation with disease activity and other markers of inflammation. Patients with active disease had significantly higher levels of serum S100A8/A9 (median ± SD) than those in the control group at the beginning of the disease (5740 ± 3157 ng/mL vs. 1447 ± 858.3 ng/mL; p < 0.0001), but also three months and six months after disease onset (p < 0.001). There was a positive correlation between S100A8/A9 serum levels and disease activity (p = 0.0003). Patients with active disease had significantly higher levels of S100A8/A9 than those in remission three months after disease onset (p = 0.0260). There was a correlation between S100A8/A9 and C-reactive protein, the C3 component of complement, ferritin, and fibrinogen. Serum levels of S100A8/A9 were also higher in patients with greater skin areas covered with rash. We demonstrated that serum levels of S100A8/A9 correlated well with disease activity and other biomarkers of inflammation in children with IgAV. According to our results, serum S100A8/A9 may be a good indicator of active disease in IgAV.
Molecular and Cellular Involvement in CIPN
Authors: Kacem Housem , Cimini Annamaria , d’Angelo Michele , Castelli Vanessa
Published: March 2024

Many anti-cancer drugs, such as taxanes, platinum compounds, vinca alkaloids, and proteasome inhibitors, can cause chemotherapy-induced peripheral neuropathy (CIPN). CIPN is a frequent and harmful side effect that affects the sensory, motor, and autonomic nerves, leading to pain, numbness, tingling, weakness, and reduced quality of life. The causes of CIPN are not fully known, but they involve direct nerve damage, oxidative stress, inflammation, DNA damage, microtubule dysfunction, and altered ion channel activity. CIPN is also affected by genetic, epigenetic, and environmental factors that modulate the risk and intensity of nerve damage. Currently, there are no effective treatments or prevention methods for CIPN, and symptom management is mostly symptomatic and palliative. Therefore, there is a high demand for better understanding of the cellular and molecular mechanisms involved in CIPN, as well as the development of new biomarkers and therapeutic targets. This review gives an overview of the current knowledge and challenges in the field of CIPN, focusing on the biological and molecular mechanisms underlying this disorder.
The Expression Patterns of Immune Checkpoint Molecules in Colorectal Cancer: An Analysis Based on Microsatellite Status
Authors: An Sanghyun , Li Wanlu , Do Hyejin , Kwon Hye Youn , Kim Bora , Kim Kwangmin , Kim Youngwan , Cho Mee-Yon
Published: March 2024

Recently, immunotherapy has arisen as a novel treatment approach for patients with colorectal cancer (CRC), but the effectiveness of immunotherapy varies in these patients. We hypothesized that immune checkpoint molecules (ICMs), which are the targets of immunotherapy, are often exhibited concomitantly. Our objective was to investigate the patterns of ICM expression in patients with CRC and the differences in ICM expression based on microsatellite instability status. The immunohistochemical expression of programmed cell death protein 1 (PD-1), programmed cell death ligand 1 (PD-L1), T cell immunoglobulin and mucin-domain containing-3 (TIM-3), and lymphocyte-activation gene 3 (LAG-3) in the tumor center and periphery was assessed in patients with non-metastatic colorectal cancer. We enrolled 83 patients with CRC: a total of 40 microsatellite-stable (MSS) and 43 microsatellite-instability-high (MSI-H) cancer patients. PD-L1 was more frequently expressed in the tumor center in the MSI-H patients with than that in the MSS patients (18 [41.9%] vs. 3 [7.5%], respectively; p < 0.001), and the same trend was observed for TIM-3 expression (30 [69.8%] vs. 19 [47.5%], respectively; p = 0.047). The concomitant expression of two or more ICMs was more frequently observed than no expression or the expression of a single molecule in both the MSS and MSI-H groups; a total of 34 (79.7%) patients with MSI-H cancer and 23 (57.5%) with MSS cancer showed ICM expression at the tumor center, whereas 34 (79.7%) patients with MSI-H cancer and 22 (55%) with MSS cancer showed expression at the tumor periphery. Patients with the genetic characteristics of MSI-H cancer showed higher expression levels of ICMs than those in patients with MSS cancer, and predominantly, two or more ICMs were concurrently expressed. Our findings highlight the potential efficacy of the dual-blockade approach in immunotherapy, particularly in patients with MSI-H CRC.