bluuebunny/crossref_metadata_2025 huggingface dataset:
Biomechanical study of partial lateral corpectomy with or without an association with pediculectomy and hemilaminectomy in canine specimens
Authors: Costa S.D.P. , Araújo B.M. , Cardoso P.H.N. , Silva D.O.B. , Olivier N.C. , Tudury E. Alberto , Baraúna Junior D.
Published: February 2023

ABSTRACT The present objective was to increase the number of biomechanical cycles performed using non-chondrodystrophic dog specimens, based on the study by Araújo (2017), comparing partial lateral corpectomy (PLC) alone, corpectomy and pediculectomy, and PLC associated with pediculectomy and hemilaminectomy to determine if there are significant differences between them regarding deformation versus applied force. Groups were divided as: control (G1), corpectomy (G2), corpectomy + pediculectomy (G3), and corpectomy + pediculectomy + hemilaminectomy (G4). The level of displacement versus force was observed during axial compression, flexion, extension, right and left lateral bending, and right and left axial rotation. Significant differences were observed between groups for flexion, extension, right and left axial rotation, and left lateral bending, whereas there was no difference for axial compression and right lateral bending. PLC and PLC with pediculectomy had significant differences in flexion and in extension, similar to PLC associated with pediculectomy and with hemilaminectomy in right and left axial rotation, flexion, extension, and right lateral bending.
Fixed bilateral endo-exo prostheses in feline femur - case report
Authors: Mendonça A.G.C. , Braga V.A.A. , Fernandes T.H.T. , Oliveira G.C.R. , Beraldo G.S.
Published: February 2023

ABSTRACT The use of endo-exo prosthesis is an alternative to improve the quality of life of amputees, allowing correct distribution of body weight, reducing skin problems and pain related to the implant, and reducing lameness. The aim of this paper is to report the use of a fixed bilateral endo-exo femur prostheses in a cat with amputation prior to treatment. The endoprostheses were made with titanium and the exoprostheses were made with 3D printing technology. A longitudinal craniolateral surgical approach was used and the skin incision was made over the knee-joint, then fascia lata incision, followed by biceps femoris and vastus lateralis muscles retraction, incision through the joint capsule to expose the tibiofemoral joint, disarticulation, and tibia and fibula removal. Afterwards, a femoral leveling osteotomy and insertion of the implant in the medullary canal were performed, being attached in the cis and trans cortical bone. The patient was evaluated in the immediate post-operative and periodically thereafter, showing satisfactory results, without macroscopic signs of rejection, wound dehiscence or infection, fracture, or injury of limbs, and presented adapted walking. Furthermore, it was observed the return to common behaviors for the species.
Conexões históricas entre as políticas de rastreamento do câncer de colo do útero e a educação profissional em citopatologia no Brasil
Authors: Medrado Leandro , Lopes Renato Matos
Published: February 2023

Resumo Os técnicos em citopatologia são fundamentais para o rastreamento do câncer de colo do útero, porém existem lacunas na sua formação. Nesta revisão de literatura, com abordagem qualitativa, realizada na Biblioteca Virtual em Saúde e na SciELO, sem recorte temporal, descrevemos o histórico das políticas públicas de combate ao câncer de colo de útero, relacionando-o à história da educação profissional técnica em citopatologia, analisando as conexões entre tais sequências de eventos. Numa perspectiva histórica, a educação profissional em anatomia patológica se deu principalmente pela formação em serviço, porém o campo da formação técnica em citopatologia destoa desse modelo. Esforços diversos para estruturar os currículos na área e reduzir as discrepâncias nessa formação foram realizados. Contudo, disputas entre interesses político-econômicos, marcando a divisão técnica do trabalho no país, somadas a embates corporativos no trabalho em citopatologia, e o descompasso entre ações de educação e de rastreamento impediram a materialização efetiva desses esforços. A demanda pela incorporação de novas tecnologias acentua ainda mais a fragilidade na formação da categoria. Somente com ações governamentais concretas de formação e de regulamentação profissional será possível transformar esse cenário, favorecendo o desenvolvimento e a ampliação das campanhas de detecção precoce do câncer.
Feito Moby-Dick: Uma leitura organizacional da monomania privatista de Paulo Guedes como estratégia discursiva da liderança autoritária neoliberal no Brasil
Authors: Santos e Carvalho Ricardo Vinicius C. dos
Published: February 2023

Resumo A liderança autoritária é um desafio à análise organizacional. A perplexidade sobre como aceitamos que ela tenha ocorrido diversas vezes na história perpassa o pensamento de intelectuais como Adorno, Habermas, Sloterdijk e Arendt. Uma maneira complementar de estudá-la é por meio da literatura. A literatura possui enorme potencial interpretativo do mundo que, dada a criatividade de suas autoras e autores, consegue trabalhar temas complexos, personagens, eventos, com uma liberdade que a ciência, pelo seu rigor metodológico, muitas vezes não se permite. Como organizações são também constituídas discursivamente, os elementos textuais da literatura nos permitem uma liberdade epistemológica, ensejando análises que consigam abordar temas organizacionais tradicionais, como a liderança, de outra maneira. A proposta deste ensaio teórico é valer-se de um clássico da literatura, o romance Moby-Dick , para a partir dele realizar uma análise crítica do discurso, baseada em Norman Fairclough, da liderança monomaníaca neoliberal-autoritária de Paulo Guedes, ministro da Economia do governo Jair Bolsonaro. O objetivo é demonstrar o potencial e a riqueza do uso da literatura combinada à análise do discurso para a compreensão de fenômenos organizacionais. A interpretação será guiada pelo estilo de liderança da personagem Acab, capitão do navio Pequod, que devido à sua obsessão por caçar a cachalote branca gigante, Moby Dick, conduz sua embarcação a um desfecho trágico, similar ao que vem ocorrendo ao Brasil devido ao ódio ao Estado presente nos discursos de Paulo Guedes.
O Organizar de Práticas de Gestão de Atividades Artísticas e Culturais em uma Escola pública
Authors: Rezende Bruno Ricardo Peixoto de , Faustino Junior Dério José
Published: February 2023

Resumo Este artigo objetivou analisar o organizar das práticas de gestão de atividades artísticas e culturais em uma instituição federal de ensino. Para isso, utiliza as contribuições do organizing e da prática como lente como abordagens epistemológicas e se caracteriza como uma pesquisa de natureza qualitativa, desenvolvida em duas fases: uma exploratória, que envolve análise de documentos e duas entrevistas projetivas, e uma fase de imersão em campo, caracterizada pelo método da observação participante e pela análise das práticas de gestão na implantação de um Núcleo de Arte e Cultura na instituição federal de ensino estudada. Como resultados, obteve-se que a abordagem do organizing articulada às práticas de gestão de atividades artísticas e culturais possibilita entender a gestão de maneira menos ortodoxa em relação ao binarismo comum ao campo da gestão escolar, que envolve constante disputa entre elementos e ideologias da gestão democrática e da gestão tradicional. O estudo contribui por caracterizar o processo de ressignificação dos aspectos materiais na prática da gestão escolar, o que possibilita problematizar o uso dos planejamentos administrativos e o papel da contextualidade na atribuição de competências das dinâmicas grupais e apontar os efeitos das tomadas de decisões na gestão escolar.
Citizen Participation and Municipality Size: Findings in Light of Demonstrations between 2015 and 2017
Authors: Sabioni Marjorie , Reis Anderson de Oliveira
Published: February 2023

Abstract Internet democratization and the natural outspread of news reports and information in social media have been changing citizen’s participation at public sphere. The complexity of the current scenario takes to reflections about how participation practices have been taking place in municipalities. Thus, the aim of the present study is to assess what demonstrations show about municipality size and citizen participation within the Brazilian context. A quantitative-qualitative research schedule was developed in order to do so. The qualitative stage of it aimed at facing information gotten from effective participation in demonstrations carried out in municipalities. Results have shown that structural discrepancies among municipalities translate differences seen in citizen participation. If one takes into account what would be the best municipality size for the democratic exercise, the herein analyzed contextual aspects point toward the coexistence of fruitful environments to participation either in small- or large-sized municipalities, although there is the trend of concrete participation in larger municipalities.
Management of a Space for Speech and Analysis of the Conditions for the Production of Versions regarding a Corporate Crime: the Case of the Vale S/A B1 Dam Failure in Brumadinho (MG)
Authors: Alves Elizeu Barroso
Published: February 2023

Abstract This paper analyzes how the construction occurred of the conditions for the production of versions of the corporate crime related to the Vale S/A dam failure in Brumadinho (MG) based on an analysis of the final report of the Parliamentary Commission of Inquiry of the Chamber of Deputies (CPI Bruma). This study provides a specific contribution by presenting corporate crime as a crime legitimized by management, in which the dynamic of organizations operated under its logic influences the criminal practices as it exerts pressure for economic results at any cost. Thus, our study aimed to analyze how the construction occurred of the conditions for the production of versions regarding the corporate crime and the discursive dynamic around the corporate crime of Vale S/A in Brumadinho (MG), in the CPI Bruma. Methodologically, we sought to use documental research in the investigative process and the final report of the CPI Bruma, created specifically to investigate the failure of the B1 dam at the Córrego do Feijão mine, applying discourse analysis from a habermasian perspective, seeking to demonstrate that communicate practices may be intentionally distorted to favor one group to the detriment of another. With this, we analyzed the management of the space for speech in the CPI, through the habermasian lens, the multiple discourses, under the premises of the validity intentions of the act of speech, and its communicative distortions. We provide indications from the CPI Bruma report that suggest that the failure of the B1 dam at the Córrego do Feijão mine was not an accident, but occurred because of the negligence of Vale S/A, which, in pursuing financial success, chose to prioritize economic results and ignore the imminent danger of failure.
Gestão de Espaço de Fala e Análise das Condições de Produção de Versões sobre um Crime Corporativo: O Caso do Rompimento da Barragem B1 da Vale S/A em Brumadinho (MG)
Authors: Alves Elizeu Barroso
Published: February 2023

Resumo O presente trabalho analisa como foram construídas as condições de produção de versões do crime corporativo relacionado ao rompimento da barragem da Vale S/A em Brumadinho (MG) a partir da análise do relatório final da Comissão Parlamentar de Inquérito da Câmara dos Deputados (CPI Bruma). Este estudo traz uma particular contribuição ao apresentar o crime corporativo como o crime legitimado pelo management , no qual a dinâmica das organizações operadas sob sua lógica influencia as práticas criminosas à medida que pressiona por resultados econômicos a qualquer custo. Assim, nosso estudo teve por objetivo analisar como se construíram as condições de produção de versões sobre o crime corporativo, a dinâmica discursiva em torno do crime corporativo da Vale S/A em Brumadinho (MG), na CPI Bruma. Metodologicamente, buscamos nos orientar pela pesquisa documental no processo investigativo e pelo relatório final da CPI Bruma, criada especificamente para investigar o rompimento da barragem B1 da Mina Córrego do Feijão, aplicando uma análise de discurso de perspectiva habermasiana, visando demonstrar que as práticas comunicativas podem ser distorcidas intencionalmente em benefício de um grupo e em detrimento de outro. Com isso, analisamos a gestão do espaço de fala na CPI, sob o prisma habermasiano, os múltiplos discursos, sob as premissas das pretensões de validez do ato de fala, e suas distorções comunicativas. Apontamos indícios do Relatório da CPI Bruma que sugerem que o rompimento da Barragem B1 da Mina Córrego do Feijão não foi um acidente, mas ocorreu por conta da negligência da Vale S/A, que, visando ao seu êxito financeiro, optou por priorizar os resultados econômicos e ignorar o perigo iminente do rompimento.
Occupational therapy from an ecosocial perspective of mental health
Authors: Mansilla Oscar Quiroz , Ojeda Cristian Paredes , Neira Pablo , Algado Salvador Simó
Published: February 2023

Abstract It is described the experience of a therapeutic garden project located in the vicinity of the Psychiatry Service of the Guillermo Grant Benavente Hospital (HGGB) in Concepción, Chile, led by the Non-Governmental Organization Rebrota (ONG Rebrota) based on the ecosocial perspective applied in people with mental health needs. It aims to analyze this Eco-social Occupational Therapy experience as a practical tool for the transformation of territories, allowing occupational therapists to understand the interaction between society and nature as a socio-ecological process that impacts different spheres of human beings, especially in social participation and mental health. Using the descriptive methodology of the Eco-social Entrepreneurship Process Model, we carry out a practical orientation of actions so that communities, inserted in their natural and cultural environments, can discover themselves reflexively, generating collective health processes. Eco-social Occupational Therapy develops practices that improve health and promotes the well-being of individuals, groups and/or communities, generating a positive impact on socio-ecosystems and building healthier, more inclusive, and sustainable communities. Eco-social Occupational Therapy is an invitation to generate research-action projects to mitigate the social impact on nature in a context of the climate crisis, where we find communities in the genesis of transcultural knowledge that allows us to develop a transformative praxis for good living in tune with our environment; while improving their well-being and participation, from a human rights and occupational justice perspectives.
QUANDO A EDUCAÇÃO INFANTIL É NA ALDEIA: NARRATIVAS DE PROFESSORES INDÍGENAS EM FORMAÇÃO
Authors: Bettiol Célia Aparecida , Mubarac Sobrinho Roberto Sanches
Published: February 2023

RESUMO Este artigo visa refletir sobre a educação infantil no processo de formação de professores na educação escolar indígena. Tem como base as narrativas de professores no curso de Pedagogia Intercultural Indígena no Vale do Javari (AM), Brasil. Tais narrativas foram produzidas a partir das disciplinas “Infâncias e Escola Indígena” e “Estágio Supervisionado”, as quais foram organizadas em três procedimentos: as narrativas pessoais da própria infância; as narrativas por etnia, nas quais se destacaram a forma como as crianças vivem nas comunidades; e as narrativas sobre a criança nas escolas indígenas de educação infantil.
Integrating Cutting-Edge Methods to Oral Cancer Screening, Analysis, and Prognosis
Authors: Dholariya Sagar , Singh Ragini D. , Sonagra Amit , Yadav Dharamveer , Vajaria Bhairavi N. , Parchwani Deepak
Published: February 2023

Oral cancer (OC) has become a significant barrier to health worldwide due to its high morbidity and mortality rates. OC is among the most prevalent types of cancer that affect the head and neck region, and the overall survival rate at 5 years is still around 50%. Moreover, it is a multifactorial malignancy instigated by genetic and epigenetic variabilities, and molecular heterogeneity makes it a complex malignancy. Oral potentially malignant disorders (OPMDs) are often the first warning signs of OC, although it is challenging to predict which cases will develop into malignancies. Visual oral examination and histological examination are still the standard initial steps in diagnosing oral lesions; however, these approaches have limitations that might lead to late diagnosis of OC or missed diagnosis of OPMDs in high-risk individuals. The objective of this review is to present a comprehensive overview of the currently used novel techniques viz., liquid biopsy, next-generation sequencing (NGS), microarray, nanotechnology, lab-on-a-chip (LOC) or microfluidics, and artificial intelligence (AI) for the clinical diagnostics and management of this malignancy. The potential of these novel techniques in expanding OC diagnostics and clinical management is also reviewed.
Sacituzumab govitecan expands its therapeutic spectrum among breast cancer subtypes
Authors: Vranic Semir
Published: February 2023

Antibody-drug conjugates (ADCs) are novel, highly potent drugs composed of a small molecule of an anticancer drug (payload)attached to humanized antibody recognizing an epitope on the surface of cancer cells. ADCs are rapidly expanding in the oncology field. By 2022, >180 ADC-based clinical trials have been conducted [1]. Most of these clinical trials are in phases I or II [1]. Several ADCs have been approved and used for the treatment of various malignancies (e.g., brentuximab vedotin (BV) for the treatment of CD30+ lymphomas, trastuzumab emtansine (T-DM1) for advanced/metastatic/or early-stage high-risk HER2-positive breast cancer with residual disease after neoadjuvant treatment) [2].
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Адаптация методики отношения к неопределенности Р. Нортона MAT-50: анализ внутренней структуры
Authors: Злобина М.В.
Published: February 2023

Проблема отношения человека к неопределенности является одной из наиболее актуальных проблем современности. В этой связи возрастает необходимость поиска адекватных и корректных способов измерения и диагностики данного конструкта. Самым распространенным методом оценки толерантности и интолерантности к неопределенности на настоящий момент являются опросники. В их числе — разработанная в 1975 г. Р. Нортоном шкала толерантности к неопределенности MAT-50 (Measurement of Ambiguity Tolerance). Не­смот­ря на то что оригинальная методика обладает высокими психометрическими показателями и позволяет оценивать толерантность к неопределенности в широком круге ситуаций, она не была адаптирована на русскоязычной выборке. Данная работа представляет первый этап адаптации данной методики на русскоязычной выборке, а именно — анализ внутренней структуры опросника. В работе также проведена оценка ретестовой надежности и конвергентной валидности опросника. На выборке из 843 человек было показано, что шкала обладает высокой внутренней согласованностью: из восьми субшкал четыре достигли хорошего уровня внутренней согласованности. В результате конфирматорного факторного анализа не удалось подтвердить оригинальную восьмифакторную модель опросника. Эксплораторный факторный анализ методом главных компонент выявил восемь факторов, объясняющих 41.9% дисперсии. Четыре фактора хорошо интерпретируются в соответствии со следующими субшкалами: «Публич­ный образ», «Решение проблем», «Социаль­ная сфера», «Привычки». Было показано, что субшкалы опросника в целом обладают хорошей ретестовой надежностью и валидностью. Адаптация методики MAT-50 на русскоязычной выборке позволит существенно дополнить и расширить данные, получаемые с помощью существующих на русском языке методик толерантности к неопределенности.
Методика «Жизненная успешность»: структура и валидизация
Authors: Деева Н.А.
Published: February 2023

В статье представлено описание процесса разработки и результатов валидизации новой методики «Жизненная успешность». Методика направлена на изучение компонентов жизненной успешности личности как метаресурса в системе саморегуляции. Теоретический конструкт методики разработан на основе результатов анализа социально-философских, теоретических и прикладных психологических исследований, которые позволили подойти к пониманию жизненной успешности как триадического системно-функционального образования в системе саморегуляции личности, проявляющегося в виде механизма, свойства и состояния. Этот метаресурс позволяет личности рефлексивно соотносить личные цели, ценности и смыслы с процессами и результатами субъектной активности с учетом внутренних приоритетов и складывающихся социокультурных условий. Методика состоит из четырех частей, каждая из которых измеряет определенный компонент жизненной успешности (рефлексивные механизмы, интегративные качества, эмоциональные переживания, ценности). Исслед­о­вание показало (N = 705), что части обладают хорошей внутренней согласованностью (a Кронбаха 0.789–0.897), конструктной и конвергентной валидностью. Конст­руктная валидность подтвердилась в результате проверки моделей при помощи конфирматорного факторного анализа. Конвергентная валидность подтвердилась в результате корреляционного анализа со шкалами специально отобранных методик, которые характеризуют мотивационное и психологическое состояние, ресурсные возможности, выраженность субъектности, особенности осознанной саморегуляции поведения. Рес­пон­денты с высокими показателями жизненной успешности демонстрируют наиболее положительный мотивационный фон, удовлетворенность жизнью, экзистенциальную наполненность; проявляют такие ресурсные возможности, как жизнестойкость и готовность к изменениям; имеют высокие показатели субъектности. Неожиданным фактом явилось наличие отрицательных корреляций показателей жизненной успешности с некоторыми стилистическими показателями осознанной саморегуляции.
Программа «Земский доктор» как фактор миграции врачей на периферию регионов Центрального федерального округа
Authors: Шарепина Екатерина Александровна
Published: February 2023

Программа «Земский доктор» (далее Программа) в 2022 г. отмечает 10-летний юбилей, наряду с положительными эффектами исследователи и эксперты отмечают ряд проблем ее реализации и спорность достигнутых результатов. Существующие исследования конструируют социально-демографический «портрет» врачей-участников без привязки к мотивациям и направлениям переезда, что противоречит подходу рассмотрения процесса миграции как комплексного, обоснованного в теории push-pull факторов миграции Э. Ли. В данной работе представлена классификация стратегий, основанная на мотивациях к переезду (притягивающие и выталкивающие факторы); обстоятельствах, осложняющих процесс переезда; социально-демографических характеристиках врачей-мигрантов и их миграционных намерениях. Эмпирическая база исследования: биографические интервью с врачами – участниками Программы и экспертные интервью с руководителями и кадровыми службами медучреждений из 9 регионов Центрального федерального округа. В 5 из 6 выделенных стратегий переезд мотивируется совокупностью факторов и выходит за рамки только материальной мотивации. В большей степени остаются работать в медучреждениях те врачи – участники Программы, которые заинтересованы в переезде в более крупный или центральный населенный пункт или наоборот в «побеге» из предыдущего населенного пункта (используют средства Программы как стартовый капитал), а также те, кто получают выплаты при возвращении в родной населенный пункт по семейным причинам или для отработки целевого направления. Врачи, участвующие в Программе исключительно для получения материальных выплат, предпочитают формат маятниковых перемещений или оценивают свой переезд как временный. Основной практический вывод статьи заключается в том, что программы компенсационных выплат для более эффективной работы должны быть дополнены неэкономическими стимулами.
Targeting interleukin-4 and interleukin-13 in the treatment of severe eosinophilic asthma
Authors: Nightingale Syabbalo
Published: February 2023

Asthma is a chronic inflammatory airway disease affecting about 300 million people and responsible for 500,000 deaths annually globally. Eosinophilic asthma is one of the most common phenotypes of asthma. It constitutes about 50% to 60% of all cases of asthma, and it is the most common phenotype in children presenting with severe acute asthma. The mechanism of eosinophilic asthma is chronic airway inflammation which leads to airway hyperresponsiveness, and remodeling due to the immunopathological effects of inflammatory cytokines. The duet cytokines interleukin-4 (IL-4) and IL-13 play the most central role in the pathophysiology of eosinophilic asthma. The two sister cytokines are slightly similar with a 25% homology, they share a common signaling IL-4Rα chain, and have identical biological effects. Their principal biological effect is the development of Th2 cells from naïve T helper type 0 (Th0) lymphocytes. Th2 cells produce several cytokines responsible for inducing airway eosinophilic inflammation. They induce the ε isotype switch and the switching of the B cell immunoglobulin (Ig) production from IgM to IgE. Furthermore, they stimulate eosinophil proliferation, and migration to the allergic airways and promote eosinophil survival by suppressing eosinophil apoptosis. Activated eosinophils secrete several cytotoxic cationic proteins which damage the airway epithelium, and amplify the inflammatory cascade and airway remodeling. Most patients with eosinophilic asthma can achieve control on a long-acting β2-agonist, inhaled corticosteroid, and a leukotriene receptor antagonist. However, about 3.6-10% do not achieve asthma control. These patients usually benefit from treatment with a biologic. Dupilumab is the only biologic targeting IL-4 and IL-13 approved for the treatment of moderate-to-severe eosinophilic asthma. Clinical trials have shown that treatment with dupilumab results in good asthma control, and significantly reduces moderate-to-severe exacerbation rates (p < 0.001). Additionally, treatment with dupilumab has been shown to significantly improve lung function (p < 0.001), and health-related quality of life, and allows patients to taper or discontinue corticosteroid treatment.
Lactate Concentration assay (LDH method) v1
Authors: T Hass Daniel , B. Hurley James
Published: February 2023

The assay I describe measures lactate concentration in a sample. The principle of the assay is to convert lactate in a sample and excess NAD+ in the buffer to pyruvate and NADH with lactic dehydrogenase. Pyruvate is trapped by hydrazine in the buffer to form pyruvate hydrazone, effectively decreasing concentrations of free pyruvate and driving forward lactate consumption. NADH can be observed on a microplate reader because it absorbs light at 340 nm. I adapted this assay for use in a microplate reader from "A flexible system of enzymatic analysis" by Oliver Lowry and Janet Passoneau (Lactate method II). It is reliable, inexpensive, and diagnostically useful for calculating cellular lactate production rates.
PCR-NGS for RNA viruses v1
Authors: Misu Masayasu
Published: February 2023

This PCR-NGS were optimized for an NGS machine, MinION. These methods do not require nucleic acid amplification with virus-specific PCR primers, physical viral particle enrichment, and RACE. These methods enable whole RNA viral genome sequencing by combining the following techniques: 1) Removal of unwanted DNA and RNA other than the RNA viral genome by nuclease treatment. 2) The terminal of viral genome sequence determination by barcoded linkers ligation. 3) Amplification of the viral genomic cDNA using ligated linker sequences-specific PCR. This method can be exploited to determine any whole RNA viral genomes (i.e., single-stranded, double-stranded, positive-stranded, negative-stranded, non-segmented or multi-segmented genomes).
Purification of 10xHis-SuperTEV v1
Authors: Lau Kelvin
Published: February 2023

SuperTEV is a mutated version of TEV (Tobacco Etch Protease) a cysteine protease widely used in labs as it is highly specific to a cleavage sequence that can be genetically encoded. Depending on the lab, it has been reported that the protease is unstable or purifies with low yields. Efforts have been made for many years (Tropea, 2009, Methods Mol Biol) to improve its solubility. Here we report our platform's efforts in generating a new TEV variant, called SuperTEV, that incorporates 9 mutations identified by different groups in recent years applied together at once. We find that the protein is easily produced and purified in large amounts and is functional. The list of mutations on the canonical TEV protease are the following (with references that also identified the same mutations): T17S (directed evolution, increased solubility and production) van den Berg et al., 2006 and Wei et al., 2012 L56V (rational design, improved solubility) Cabrita et al., 2007 and Wei et al., 2012 N68D (directed evolution, increased solubility and production) van den Berg et al., 2006 and Wei et al., 2012 I77V (directed evolution, increased solubility and production) van den Berg et al., 2006 and Wei et al., 2012 S135G (rational design, increased solubility) Cabrita et al., 2007 and Wei et al., 2012 I138T (increased catalytic activity; TEV3) Sanchez and Ting, 2019 S153N (increased catalytic activity; TEV3) Sanchez and Ting, 2019 T180A (increased catalytic activity; TEV3) Sanchez and Ting, 2019 S219V (inhibits autoproteolysis) Kapust, 2001 References: Wei et al., 2012. Protein Expression and Purification Sanchez and Ting, 2019. Nature Methods van den Berg et al., 2006. Journal of Biotechnology Cabrita et al., 2007. Protein Science Kapust et al., 2002. Biochemical and Biophysical Research Communications
Effectiveness of the peripherally inserted central catheter for hematopoietic stem cell transplantation: a systematic review protocol
Authors: Vieira Ana Paula Gadanhoto , Duarte Bruno Kosa Lino , Urquisa Priscila Silva , Lima Maria Helena de Melo , Alves Daniela Fernanda dos Santos
Published: February 2023

Objective: to assess the effectiveness and safety of the peripherally inserted central catheter for hematopoietic stem cell transplantation. Methods: this review will follow the recommendations of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses, and the search steps will be presented through the flow diagram. The search strategy aims to locate both published and unpublished studies. No time or language restrictions will be applied. The review will consider experimental and observational studies that include adult and pediatric patients undergoing hematopoietic stem cell transplantation. Patients using peripherally inserted central catheters will be compared with those using other central catheters.
Equipe de limpeza hospitalar no contexto pandêmico: protocolo de scoping review
Authors: Silva Adaiele Lucia Nogueira Vieira da , Souza Rosely Almeida , Almeida Willian Albuquerque de , Carneiro Liliane Moretti , Rigotti Marcelo Alessandro , Ferreira Adriano Menis
Published: February 2023

Objetivo: Mapear a produção científica acerca da atuação dos profissionais de limpeza hospitalar durante a pandemia da COVID-19. Método: A busca será realizada nas bases de dados eletrônicas: PubMed / MEDLINE, Web of Science, Scopus Preview, Embase, Cochrane Library, Cumulative Index to Nursing and Allied Health Literature (CINAHL) e Biblioteca Virtual da Saúde (BVS). Para análise na literatura cinzenta será realizada pesquisa na Biblioteca Digital Brasileira de Teses e Dissertações, Google Scholar e Open Gray, sem aplicação de filtros. Os critérios de elegibilidade serão: estudos disponíveis na íntegra, publicados a partir de dezembro de 2019, e que respondem à questão de pesquisa proposta. Estudos em andamento ou preprint serão excluídos. Será realizada a avaliação do título e do resumo de todos os estudos localizados, com base nos critérios de elegibilidade. Os dados extraídos serão apresentados em diagramas ou no formato tabular, sendo acompanhados por um resumo narrativo.
Diseño instruccional basado en aprendizaje social y TPACK para mejorar el aprendizaje de idiomas
Authors:   Universidad César Vallejo, Montoya Muñoz Gabriela Emma Edith
Published: February 2023

La tecnología juega un papel importante en la educación hoy en día. Todos somos conscientes de su importancia y existen numerosas investigaciones sobre lo beneficiosa que puede ser cuando se aplica al proceso de enseñanzaaprendizaje; sin embargo, dado que las redes sociales han sido desarrolladas hace relativamente poco tiempo, no existen muchas investigaciones al respecto en el mundo, y prácticamente ninguna en el Perú. Esta investigación fue desarrollada con el único propósito de analizar el impacto que la aplicación de un diseño instruccional que promueve el uso de las redes sociales, basado en la teoría del aprendizaje social y el marco de trabajo TPACK tiene en la mejora del aprendizaje del idioma inglés de los estudiantes que cursan el Beginners IV en el Centro de Idiomas Señor de Sipán (SSLC) durante octubre de 2015. Para probar la hipótesis se aplicó el diseño cuasi experimental. Se realizó un Pre - test para diagnosticar el problema. Los resultados mostraron que los estudiantes tenían un bajo nivel de aprendizaje del idioma inglés, no eran capaces de comunicarse en el idioma de destino ni siquiera en un nivel básico. Se realizaron más investigaciones, para profundizar en el problema y después de aplicar una entrevista y un cuestionario a los profesores, fue posible detectar el problema: las actividades, estrategias y métodos aplicados en la mayoría de las clases eran tradicionales y no utilizaban adecuadamente la tecnología. Las clases no satisfacían los intereses de los estudiantes y no involucraban su participación. Los resultados fueron alentadores, después de la aplicación del Post - test se pudo observar la mejora del nivel de los estudiantes y el interés por sus clases de inglés.
Instrumento de canal de flujo siguiendo el modelo del consistómetro Bostwick
Authors:   Universidad Nacional de Frontera, Miranda Zamora William Rolando , Sánchez Chero Manuel Jesús ,   Universidad Señor de Sipán, Sánchez Chero José Antonio ,   Universidad César Vallejo
Published: February 2023

En la tecnología de los alimentos se registra la medida de la consistencia de muchos productos espesos o pastosos como las salsas o pastas de frutas o vegetales. El problema ingenieril radica en el diseño y la construcción de un dispositivo de flujo que permita evaluar la consistencia de los alimentos pastosos o espesos, para lo cual en realidad se ha reformulado como un dispositivo empírico de canal de flujo siguiendo el modelo de consistómetro Bostwick mediante la “consistencia Bostwick”, que es ampliamente usada por los investigadores para definir términos técnicos, como por ejemplo la salsa de tomate. El objetivo ha sido diseñar y construir un instrumento, en donde se libera el alimento espeso o pastoso en un canal especialmente fabricado y se mide la distancia que el alimento se ha deslizado después de un intervalo de tiempo determinado, normalmente 30 segundos; esto le da la “consistencia Bostwick” al alimento espeso o pastoso, por ejemplo, un puré de tomate, B30 (Bostwick 30 segundos) sólo puede ser considerado como salsa de tomate siempre que tenga una medida Bostwick de menos de 30 cm a 20°C, de acuerdo con organismos gubernamentales. Un instrumento siguiendo el modelo del Bostwick se ha diseñado y construido de acero inoxidable.
Técnicas de afrontamiento para disminuir el síndrome de Burnout
Authors:   Universidad César Vallejo, Gómez Uriarte Miriam Alexandra , Carbajal Cornejo Katherine ,   Universidad Santo Toribio de Mogrovejo
Published: February 2023

En la empresa Imperios Operadores Logístico S.A, se aplicó un cuestionario del Síndrome de Burnout a 28 colaboradores participantes, cuyo diagnóstico arrojó que el 39% se ubicó en el nivel medio y el 61% en nivel alto del síndrome; señales de que dichos colaboradores se encontraban cansados emocionalmente, desmotivados e indiferentes con sus compañeros de trabajo. Caso contrario ocurrió en el post test, cuyos resultados alcanzados, muestran que ningún colaborador tiene un nivel alto de Síndrome de Burnout; sino más bien que el 25% de los evaluados lograron tener un nivel bajo, mientras que un 75% se ubicó en un nivel medio, por tal razón la aplicación de las técnicas de afrontamiento desarrolladas con dichos colaboradores lograron disminuir los niveles altos del Síndrome de Burnout. En tal sentido se afirma que las técnicas de reestructuración cognitiva, manejo de situaciones y técnica de solución de problemas propuestas por Majoney y Arnkoff (1978), permitieron disminuir dicho síndrome, cuyas dimensiones propuestas por Maslach y Jackson (1981b) fueron agotamiento emocional donde un 14% ubicado en nivel bajo mejoro considerablemente a un 75%, mientras que la dimensión de despersonalización de un 64% ubicado en el nivel bajo este alcanzó un 86%, respecto a la dimensión realización personal de un nivel alto de 0% este logro aumentar a un 68%, Finalmente se concluye que las técnicas de afrontamiento aplicadas fueron efectivas alcanzando un nivel de significancia de p_valor = 0,001<0.05 afirmando que dichas técnicas lograron disminuir el Síndrome de Burnout.
Actitud hacia la investigación en estudiantes de maestría de ciencias de la salud y ciencias sociales
Authors:   Universidad Norbert Wiener, Yace Martínez Jessica Maryluz , Arispe Alburqueque Claudia Milagros ,   Universidad Norbert Wiener, Diaz Del Olmo Morey Fernando Luis ,   Universidad Norbert Wiener, Yangali Vicente Judith Soledad ,   Universidad Norbert Wiener, Calla Vasquez Kriss Melody ,   Universidad Norbert Wiener
Published: February 2023

Objetivos. Determinar el nivel de actitud hacia la investigación científica y hacia la estadística en estudiantes de las maestrías de ciencias de la salud y ciencias sociales, así como la identificación de sus antecedentes académicos. Materiales y métodos. Se realizó un estudio cuantitativo, observacional, analítico y aplicado. Resultados Se encontró que 70,5% de estudiantes son de sexo femenino, 57,6% si tiene hijos, el 98,5% estudia y trabaja actualmente, el 41,7% proviene de otras universidades privadas distintas a la de origen, 58,3% se graduó bajo la modalidad de tesis, el 86,4% sólo cuenta con grado académico bachiller, 17,4% ha realizado alguna publicación científica y el 10,6% ha realizado una publicación en alguna revista indizada, los alumnos que más investigación realizan son los de la maestría en ciencias sociales (docencia universitaria), el 59,8% de todos los estudiantes presenta una actitud favorable hacia la investigación científica y el 62,9% de todos los estudiantes presenta una actitud favorable hacia la estadística, los alumnos de la maestría en ciencias sociales (docencia universitaria) presentaron mejores niveles de actitud favorable hacia la investigación científica y estadística en comparación a la maestría en ciencias de la salud (gestión en salud) Conclusiones: Los estudiantes de maestría de ciencias de la salud y ciencias sociales presentan una actitud favorable hacia la investigación científica y hacia la estadística.
Comparison of microvascular endothelial function as measured by laser Doppler flowmeter among non-smoker and smoker males
Authors: Srinivasamurthy Suresh Kumar , Bairy Laxminarayana Kurady , Naduvil Sareesh Narayanan
Published: February 2023

Background: To understand the role of smoking in influencing endothelial function as assessed by LDF among non-smoker and smoker males.
Methods: The LDF measurement for a total of 35 non-smokers and 16 smokers was done in the central research laboratory after written informed consent. The change in LDF signal in response to acetylcholine 100 µl, which was delivered to the forearm skin by iontophoresis, was measured as perfusion units (PU).
Results: The pre-ACh LDF signal were statistically not significant between the groups. The increase in LDF signal was more prominent in non-smoker group. The LDF signal parameters such as differences in minimal response pre and post-ACh; difference in mean response pre and post ACh; the difference in maximal response pre and post ACh was not statistically significant between groups. However, the difference in the area under curve (AUC) pre and post-ACh (PU.min) (non-smoker 20089.34 (3438.92) vs smoker 13220.72 (3379.52); p=0.16) showed a trend towards statistical significance.
Conclusions: Microvascular endothelial function as assessed by LDF signal among smokers (pack-years;1.9±1.44) and non-smokers is statistically insignificant. However, lower microvascular endothelial function is observed among smokers.
Ten years of survival among early-stage breast cancer patients: a hospital-based study
Authors: Nahar Shamsun , Jamal Uddin  , Saidul Haque  , Nazir Uddin Mollah  , Sarwar Alam  , M. A. Bari  , Faruk Ahmed  , Khursheda Akhtar  , Mahmudur Rahman
Published: February 2023

Background: The incidence rate of breast cancer is gradually increasing all over the world. In Bangladesh, we have very little research-based information regarding the survival of patients with early-stage breast cancer. The aim of this study was to determine the survival outcome of early-stage (Stages I- IIIA) breast cancer among female patients in respect to their age group, stage of the disease, tumor grade, nodal status, and hormone receptor status
Methods: This was a prospective observational study focused on the survival outcome conducted in the department of clinical oncology, Bangabandhu Sheikh Mujib medical university (BSMMU), Dhaka, Bangladesh during the period from December 2011 to December 2021. One hundred and five (105) female patients with early-stage (Stages I-IIIA) breast cancer were selected following the inclusion and exclusion criteria as the study subjects. Properly informed written consent was taken from all the participants before starting data collection. All data were processed, analyzed, and disseminated by MS office and SPSS programs as per need.  
Results: The survival rate was found 79% (n=83). We observed stage-wise 10-year overall survival among the participants where 51.60%, 36.10%, and 12.30% survived from tumor stage I, II, and III respectively. We also observed that 29%, 60% and 11% of patients had survived from tumor grade I, II, and III respectively (Out of 83 survival cases). On the other hand, among the 83 10-year survival patients, 53.8% were of negative nodal status whereas 34.20% were of positive nodal status patients. Among the survived 83 patients, 86% were from >40 years’ age group whereas the rest 14% were from <40 years of age group.
Conclusions: Early detection and treatment of breast cancer provide a definitive survival benefit. The results of the study showed that comprehensive screening and early detection of breast cancer is required to improve the survival outcome.
Clinical spectrum of dengue fever in patients admitted to a tertiary care hospital in Bangladesh
Authors: A. F. M. Helal Uddin  , M. Lutfur Rahman  , M. Abdul Alim  , M. Mahbub H. Khan  , Shams-El-Arefin  , Kamrun Nahar  , Mohammad M. Hossain
Published: February 2023

Background: Dengue fever is a viral infection transmitted by mosquitoes and is a significant public health problem in many tropical and subtropical countries, including Bangladesh. The clinical spectrum of dengue fever can vary widely, ranging from asymptomatic infection to severe and potentially life-threatening illness. In this study, we aimed to describe the clinical spectrum of dengue fever in patients admitted to a tertiary care hospital in Bangladesh. The aim of the study was to observe the clinical spectrum of dengue fever patients.
Methods: This prospective observational study was conducted at the department of medicine, Sir Salimullah Medical College and Mitford Hospital, Dhaka, Bangladesh. The study duration was 7 months, from June 2022 to December 2022. During this period, a total of 65 cases of dengue fever cases admitted to the hospital for treatment were included in the study, who had a hospital stay of at least 3 days.
Results: The mean age of the participants was 30.78 years, and the male: female ratio was 1:1.95. 86.15% of the participants had no comorbidities, while diabetes and hypertension were observed in 6.15% of patients each. Patients' mean temperature had been high at the first check-up and dropped slowly to normal levels throughout the following check-ups, but the mean platelet count had significantly dropped from the first check-up throughout the following check-ups, improving only at the 6th check-up. ≤20,000 platelet count was observed in 6.15% at the first check-up, 9.23% at the 2nd check-up, 15.38% at the 3rd, 5.41% at the 4th, 15% at the 5th, and 0% at the 6th check-up. The most common warning signs were a positive tourniquet test (21.54%), vomiting (16.92%), and abdominal pain (15.38%). Hospital stay duration was <1 week for 95.38% of participants, and during discharge, only 3 patients had minor complications, with 3.08% mortality.
Conclusions: The study observed a higher prevalence of dengue among younger age groups, and females had a higher prevalence in the study. The regular-interval check-ups revealed that dengue severity progressed slowly, and did not become worse immediately after the infection, rather continued to progress adversely slowly throughout the check-ups. Hospital stay was <1 week for the majority of the participants, and minor mortality was observed in the study.
Relationship of incidence of anterior cruciate ligament injuries and body-built Index somatotype in Indian population
Authors: Deore Sandeep , Kale Sachin , Chalak Ajit , Nalawade Vanashree , Doshi Suraj S.
Published: February 2023

Background: Incidence of ACL injuries and physical parameters like age, sex, height, weight and BMI has been studied intensely as a risk factor. Each of them poses as a different risk factor for occurrence of ACL injuries. But body-built index-somatotype considers relative proportions of fatness, muscle mass and skeletal architecture in terms of endomorphy, mesomorphy and ectomorphy in a single individual which neutralises variables like age, sex, BMI.
Methods: 100 patients were included in the study, excluding those with multiligament injuries, associated fractures around knee, mucoid degeneration of ACL and road traffic accident cases. Parameters such as skin fold thickness, circumference and epicondylar, condylar width was taken into consideration. Body built index was calculated in terms of endomorphy, mesomorphy and ectomorphy using the heath-carter anthropometric somatotype manual.
Results: We found that amongst all those have ACL injury, endomorphy was higher grade (5.49), followed by mesomorphy (4.75). Lesser grades of ectomorphs i.e., slender people were having ACL injuries. Though there was low margin of grading between the two, endomorphy was higher in ACL injured people followed by mesomorphy.
Conclusions: Endomorphs and mesomorphs have higher tendancy of ACL injury. Ectomorphs have lesser incidence of ACL injury.