bluuebunny/crossref_metadata_2025 huggingface dataset:
Understanding the Phase of Responsivity and Noise Sources in Frequency-Domain Multiplexed Readout of Transition Edge Sensor Bolometers
Authors: Farias Nicole , Adkins Tylor , de Haan Tijmen , Lee Adrian T. , Lonappan Anto , Russell Megan , Suzuki Aritoki , Siritanasak Praween , Takatori Sayuri , Westbrook Benjamin
Published: May 2024

Cosmic microwave background (CMB) experiments have deployed focal planes with $$\mathcal {O}(10^{4})$$

O
(

10
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transition edge sensor (TES) bolometers cooled to sub-Kelvin temperatures by multiplexing the readout of many TES channels onto a single pair of wires. Digital Frequency-domain Multiplexing (DfMux) is a multiplexing technique used in many CMB polarization experiments, such as the Simons Array, SPT-3 G, and EBEX. The DfMux system studied here uses LC filters with resonant frequencies ranging from 1.5 to 4.5 MHz connected to an array of TESs. Each detector has an amplitude-modulated carrier tone at the resonant frequency of its accompanying LC resonator. The signal is recovered via quadrature demodulation where the in-phase (I) component of the demodulated current is in phase with the complex admittance of the circuit and the quadrature (Q) component is orthogonal to I. Observed excess current noise in the Q component is consistent with fluctuations in the resonant frequency. This noise has been shown to be non-orthogonal to the phase of the detector’s responsivity. We present a detailed analysis of the phase of responsivity of the TES and noise sources in our DfMux readout system. Further, we investigate how modifications to the TES operating resistance and bias frequency can affect the phase of noise relative to the phase of the detector responsivity, using data from Simons Array to evaluate our predictions. We find that both the phase of responsivity and phase of noise are functions of the two tuning parameters, which can be purposefully selected to maximize signal-to-noise (SNR) ratio.
On the influence of graphene oxide and hydroxyapatite modification on alginate-based hydrogel matrix: thermal, physicochemical, and biological considerations
Authors: Lach Aleksandra , Skoczeń Monika , Ordon Klaudia , Domalik-Pyzik Patrycja , Noga Sylwia , Sekuła-Stryjewska Małgorzata , Karnas Elżbieta , Byrska Aleksandra , Jagiełło Joanna , Lipińska Ludwika , Zuba-Surma Ewa , Pielichowska Kinga
Published: May 2024

Sodium alginate (SA) hydrogels with an addition of graphene oxide (GO) and hydroxyapatite (HAp) crosslinked by calcium chloride solution were investigated as potential materials for osteochondral tissue regeneration. The influence of various ratios of the nanoadditives in the natural derived polymer matrix on the thermal, physiochemical and biological properties was studied. Two thermal characterization methods (DSC and TGA) were employed to examine the thermal properties of the materials and provide information regarding the different types of water within the hydrogel structure. These parameters are crucial for the assessing and understanding of the adsorption/desorption processes in hydrogels and also impact their biocompatibility. The effect of GO and HAp addition on thermal characteristics of alginate hydrogel is reported, as well as the nanoadditives polymer chains interaction, as evidenced by FTIR results. The compression test confirmed that the nanoadditives, uniformly dispersed in the polymer matrix, improved the mechanical properties of the hydrogels, but only up to a certain content of additives. The composite hydrogels exhibited a very low friction coefficient. Both GO and HAp also enhanced chemical stability of alginate hydrogels under in vitro conditions. Biological assays demonstrated that most of the tested hydrogel extracts were not cytotoxic to hUC-MSCs, but they can affect the proliferation rate of the cells. Developed materials may present an intriguing alternative for osteochondral tissue regeneration.
Tool-Augmented Human Creativity
Authors: Hole Kjell Jørgen
Published: May 2024

Creativity is the hallmark of human intelligence. Roli et al. (Frontiers in Ecology and Evolution 9:806283, 2022) state that algorithms cannot achieve human creativity. This paper analyzes cooperation between humans and intelligent algorithmic tools to compensate for algorithms’ limited creativity. The intelligent tools have functionality from the neocortex, the brain’s center for learning, reasoning, planning, and language. The analysis provides four key insights about human-tool cooperation to solve challenging problems. First, no neocortex-based tool without feelings can achieve human creativity. Second, an interactive tool exploring users’ feeling-guided creativity enhances the ability to solve complex problems. Third, user-led abductive reasoning incorporating human creativity is essential to human-tool cooperative problem-solving. Fourth, although stakeholders must take moral responsibility for the adverse impact of tool answers, it is still essential to teach tools moral values to generate trustworthy answers. The analysis concludes that the scientific community should create neocortex-based tools to augment human creativity and enhance problem-solving rather than creating autonomous algorithmic entities with independent but less creative problem-solving.
“Zero interaction”, ignoring and acts of omission in the school ecology: Peer ostracism from the perspective of involved adolescents
Authors: Konieczna Agnieszka
Published: May 2024

This study focuses on one type of excluding and isolating behaviour, the essence of which is the breakup of social interactions with a group member, which falls within the commonly accepted definition of ostracism. Based on interviews with 29 adolescents aged 12–18 (including 15 girls and 14 boys, all of whom were students in public schools without migrant backgrounds), an analysis of the content and typology of peer ostracism in school classrooms was conducted, emphasizing the perspective of those directly involved in the ostracism, i.e., the perpetrators. Three main dimensions and behavioural aspects related to ostracism emerged from the data: “Avoidance (Zero Interaction)”, “Expulsion from Group” and “Complete Ostracism”. The results indicate that the identified forms of ostracism rarely occur in a pure form, and are most often carried out in combination with non-verbal acts of overt exclusion. Furthermore, peer ostracism in group-class communities, which is consensual and chronic in nature, may reflect a distinct variant that qualifies for independent study. The paper also discusses directions for future research and actions to improve the social classroom environment.
Firm interrelationships: the role of firm size
Authors: O’Leary Daragh
Published: May 2024

This paper put’s forward a working conceptualisation for how the size of firms which die influences firm interrelationships where firm deaths influence future firm births. Two conceptual arguments are developed to explain the role of firm size in influencing future firm births; the resource argument and the consumer demand argument. This relationship is also empirically tested using a multilevel model to examine the relationship between the death of differently-sized firms and future firm births in European NUTS 3 regions. Findings suggest that only the deaths of larger firms appears to increase future firm births. The resource-based argument is used to explain this finding whereas the consumer demand-based argument is used to explain why the death of smaller firms seems to reduce future firm births. The dataset used in this paper’s estimation captures a large amount of activity across 14 countries and 502 NUTS 3 regions from 2008 to 2019.
Pan-cancer analysis combined with experiments predicts NNMT as a therapeutic target for human cancers
Authors: Huang Hua , Su Lianchun , Zhang Ruihao , Wu Di , Ding Chen , Chen Chen , Zhu Guangsheng , Cao Peijun , Li Xuanguang , Li Yongwen , Liu Hongyu , Chen Jun
Published: May 2024

The identification of effective therapeutic targets plays a pivotal role in advancing cancer treatment outcomes. We employed a comprehensive pan-cancer analysis, complemented by experimental validation, to explore the potential of Nicotinamide N-methyltransferase (NNMT) as a promising therapeutic strategy for human cancers. By analyzing large-scale transcriptomic datasets across various cancer types, we consistently observed upregulated expression of NNMT. Furthermore, elevated NNMT expression correlated with inferior overall survival in multiple cancer cohorts, underscoring its significance as a prognostic biomarker. Additionally, we investigated the relationship between NNMT expression and the tumor immune microenvironment, which plays a crucial role in regulating anti-tumor immune responses. To confirm the malignant functions of NNMT in tumor cells, we conducted a series of cell-based experiments, revealing that NNMT promotes cancer cell proliferation and invasion, indicative of its oncogenic properties. The integration of computational analysis and experimental validation in our study firmly establishes NNMT as a potential therapeutic target for human cancers. Specifically, targeting NNMT holds promise for the development of innovative and effective cancer treatments. Further investigations into NNMT's role in cancer pathogenesis could potentially pave the way for groundbreaking advancements in cancer treatment.
Description and management of patients with anal fissure: insights on Italian primary care setting coming from real-world data
Authors: Gallo Gaetano , Pegoraro Valeria , Trompetto Mario
Published: May 2024

To describe patients with anal fissure (AF) and their management in primary care. Retrospective study using the Italian Longitudinal Patient Database on 18 + years old subjects with AF records during ‘July 2016–June 2021’ (selection period). Index Date (ID) was the first AF record during selection period. Sub-cohorts were defined by presence/absence of prescriptions on ID of the combination of topical nifedipine 0.3% and lidocaine 1.5% (NIF/LID). Patients’ information on the 12-month period before (baseline) and after (follow-up) ID was analyzed. Subjects with AF were 8632: 14.0% had NIF/LID on ID. Mean age was 52 (± 17.2) years, there were more women in ‘ < 50 years’ group, and more men in ‘50–70’ one. Prevalences of pregnancy and immunodepression were around 5%; most common comorbidities were hypertension (29.6%) and heart disease (13.1%), while constipation and diarrhea were < 5%. Healthcare resources utilization (HRUs) increased during follow-up, but still few patients were prescribed NIF/LID (2.8%), other treatments for AF (10.3%), or proctological visits (7.7%). NIF/LID patients were younger (< 40 years people: 30.7% versus 23.9%; p value < 0.0001), and more likely to have constipation (4.3% versus 2.5%; p value < 0.001); patients without NIF/LID showed slightly higher prevalences of hypertension (30.0% versus 27.1%; p value: 0.039) and depression (4.0% versus**2.5%; p value: 0.009), and a little higher overall HRUs. Results show that general practitioners are used to manage AF. However, there is still a gap between guidelines’ recommendations and actual management. Educational campaigns on common anal problems in primary care might help further improving AF management and optimizing HRUs.
Eating Behaviours and Dietary Intake in Children and Adolescents: A Systematic Review
Authors: Maneschy Ivie , Jimeno-Martínez Andrea , Miguel-Berges María L. , Rupérez Azahara I. , Ortega-Ramiréz Ana Daniela , Masip Guiomar , Moreno Luis A.
Published: May 2024

Purpose of Review

This systematic review aimed to examine existing evidence related to associations between eating behaviours and dietary intake in children and adolescents, with a focus on the Children Eating Behaviour Questionnaire (CEBQ) and the Dutch Eating Behaviour Questionnaire (DEBQ) as assessment tools.

Recent Finding

We conducted a systematic review following PRISMA guidelines. We included observational and interventional studies published in English, Spanish, or Portuguese, that evaluated the association between eating behaviours and food and beverage intake. Thirteen studies from nine countries met the inclusion criteria, with sample sizes ranging from 62 to 4,914 individuals aged 2 to 16 years-old. Ten studies used the CEBQ, and three used the DEBQ. Our retrieved studies showed that children and adolescents engaging in food approach behaviours tend to consume foods rich in sugar and fats. However, we observed a higher consumption of fruits and vegetables. On the other hand, children and adolescents with lower engagement to food avoidant behaviours, generally exhibited a lower overall food consumption, except for snacks, which they consumed at a higher rate.

This systematic review suggests that eating behaviours play an important role in shaping dietary intake. Nevertheless, due to the heterogeneity related to eating behaviours and diet intake, it highlights the need for further research to understand these complex relationships to develop effective interventions for promoting healthy eating habits in children and adolescents.
The late Early Pleistocene site of Fuente Nueva-3 (Guadix-Baza Depression, SE Spain): a hyena latrine developed on a quicksand trap for megaherbivores?
Authors: Palmqvist Paul , Campaña Isidoro , Granados Alejandro , Martínez-Navarro Bienvenido , Pérez-Ramos Alejandro , Rodríguez-Gómez Guillermo , Guerra-Merchán Antonio , Ros-Montoya Sergio , Rodríguez-Ruiz María Dolores , García-Aguilar José Manuel , Hernández Víctor , Espigares M. Patrocinio
Published: May 2024

The late Early Pleistocene archaeological site of Fuente Nueva-3 (FN3), which lies in the Guadix-Baza Depression (SE Spain) and is dated to ~ 1.4 Ma, contributes some of the oldest evidence of hominin presence in Western Europe, including a huge tool assemblage of Oldowan tradition, manuports (i.e., unmodified stones used as percussion tools) and abundant fossils of large mammals, some of which preserve anthropogenic marks related to defleshing, butchering and marrow processing. In addition, there are bones with tooth marks produced by scavenging carnivores. The fertile layers of the FN3 section have been grouped in a lower archaeological level (LAL) and an upper archaeological level (UAL). Both levels preserve abundant skeletal remains and lithic tools. However, the LAL shows a high density of manuports, which suggests that hominin activity was more intense at this level, while the UAL preserves many remains of megaherbivores, particularly proboscideans (Mammuthus meridionalis), and almost all coprolites unearthed from the site, which points to a greater involvement of the giant hyenas (Pachycrocuta brevirostris). In this paper, we (i) test for statistical differences in the composition of the faunal assemblages preserved in the UAL and LAL; and (ii) analyze particle size in the fertile layers of both archaeological levels. Our results show that megaherbivores are comparatively overrepresented in the UAL, specially by young elephants, while other medium-to-large and large-sized ungulates, particularly equids, are more abundant in the LAL, showing a predominance of adult individuals. Concerning the sedimentology, layers 2–3 of the LAL show a predominance of silts and clays, with sands representing a minor fraction. In contrast, layer 5 of the UAL is composed of two-thirds of fine and very fine sands, with the remaining third consisting of silts and clays. These data and the paleoenvironmental reconstruction of layer 5 suggest that it may be interpreted as a paleo-quicksand in which megaherbivores were trapped due to the elevated weight per unit area supported by their feet, and their half-sunken carcasses attracted the scavengers, particularly the hyenas, which fed on these carcasses and defecated in their surroundings.
Plants against cancer: towards green Taxol production through pathway discovery and metabolic engineering
Authors: Zerbe Philipp
Published: May 2024

The diversity of plant natural products presents a rich resource for accelerating drug discovery and addressing pressing human health issues. However, the challenges in accessing and cultivating source species, as well as metabolite structural complexity, and general low abundance present considerable hurdles in developing plant-derived therapeutics. Advances in high-throughput sequencing, genome assembly, gene synthesis, analytical technologies, and synthetic biology approaches, now enable us to efficiently identify and engineer enzymes and metabolic pathways for producing natural and new-to-nature therapeutics and drug candidates. This review highlights challenges and progress in plant natural product discovery and engineering by example of recent breakthroughs in identifying the missing enzymes involved in the biosynthesis of the anti-cancer agent Taxol®. These enzyme resources offer new avenues for the bio-manufacture and semi-synthesis of an old blockbuster drug.
Anterior vertebral tethering for adolescent idiopathic scoliosis: our initial ten year clinical experience
Authors: Braun John T. , Federico Sofia C. , Lawlor David M. , Paschos Nikolaos J. , Croitoru Daniel P. , Grottkau Brian E.
Published: May 2024

Background

Anterior vertebral tethering (AVT) is a minimally invasive alternative to fusion surgery for adolescent idiopathic scoliosis (AIS) that offers the potential for definitive scoliosis treatment with the possibility of preservation of the growth, motion, function and overall health of the spine. This study represents our first ten years using AVT to treat AIS.

Methods

In this retrospective review we analyzed our first 74 AIS patients treated with AVT 2010–2020. Multiple Lenke curve types 33–70° were treated with skeletal maturity spanning Risser -1 to 5.

Results

Of 74 consecutive AIS patients treated with AVT, 52 patients (47 female, 5 male) had sufficient 2-year follow-up for inclusion. Forty-six of these 52 patients (88%) with 65 curves (35T, 30TL/L) were satisfactorily treated with AVT demonstrating curve correction from 48.6° pre-op (range 33°–70°) at age 15.1 years (range 9.2–18.8) and skeletal maturity of Risser 2.8 (range -1 to 5) to 23.2° post-op (range 0°–54°) and 24.0° final (range 0°–49°) at 3.3 years follow-up (range 2–10 years). Curve corrections from pre-op to post-op and pre-op to final were both significant (p < 0.001). The 0.8° change from post-op to final was not significant but did represent good control of scoliosis correction over time. Thoracic kyphosis and lumbar lordosis were maintained in a normal range throughout while axial rotation demonstrated a slight trend toward improvement. Skeletal maturity of Risser 4 or greater was achieved in all but one patient. Four of the 52 patients (8%) required additional procedures for tether rupture (3 replacements) or overcorrection (1 removal) to achieve satisfactory treatment status after AVT. An additional 6 of the 52 patients (12%), however, were not satisfactorily treated with AVT, requiring fusion for overcorrection (2) or inadequate correction (4).

Conclusions

In this study, AIS was satisfactorily treated with AVT in the majority of patients over a broad range of curve magnitudes, curve types, and skeletal maturity. Though late revision surgery for overcorrection, inadequate correction, or tether rupture was not uncommon, the complication of overcorrection was eliminated after our first ten patients by a refinement of indications.

Level of Evidence

IV
An identity project: a case study of two inservice elementary stem teachers’ experiences with a comprehensive professional development
Authors: Parker Carolyn A. , Lehn Nicholas
Published: May 2024

This paper describes the experiences of two teachers, Ms. Santiago and Mr. Jelenik, who participated in a comprehensive National Science Foundation-supported science, technology, engineering, and mathematics (STEM) professional learning experience. This comparative case study describes how Ms. Santiago and Mr. Jelenik leveraged the justice-oriented curriculum of a STEM professional learning opportunity to transform their identities as educators while influencing their instructional practices. The professional learning experience included access to a justice-oriented curriculum introduced through monthly content-focused experiences, regular faculty learning communities, and support from an expert onsite coach. Evidence of transformed identities and instructional practices is derived from semi-structured in-depth interviews and video recordings of classroom teaching and learning. Analysis of the interview data suggests that Ms. Santiago and Mr. Jelenik found the professional learning experience transformative and that the experience served as a pedagogical identity project. Evidence from the classroom videos suggests that Ms. Santiago and Mr. Jelenik’s instructional practices shifted, becoming more justice-oriented and student-centered. Moreover, Ms. Santiago’s practices shifted in a manner more aligned with an engaged teaching approach. We conclude that the comprehensive STEM professional learning experience served as a pedagogical identity project while positively influencing the instructional practices of Ms. Santiago and Mr. Jelenik. Implications for preservice and in-service teacher education are discussed.
Integration of wikis in education: a qualitative systematic review
Authors: Karipidis Nikolaos , Tsimperidis Ioannis
Published: May 2024

The use of Web-based technologies in education and ways they can improve teaching and learning have been the focus of interest in the educational community during the recent years. In this regard, wikis are one of the most promising technologies. Many research studies have been presented that integrate wikis in educational settings. The abundance of the corresponding research work creates the need for a systematic survey. In this survey of the research on wikis in education content analysis is used to record and compare the conclusions of 110 recent empirical studies that used wikis in educational settings. The specific studies were published from 2014 to 2021. The conducted search was not limited to a specific level of education but involved all the corresponding levels of education. The key aspects of the educational use of wikis are outlined and clarified. Furthermore, information and suggestions that will be useful to future researchers, as well as teachers who want to integrate wikis into their teaching practice are provided. To the best of our knowledge, there is no other survey of the research that discusses all such aspects regarding wikis in education.
The Short Version of the Process-Oriented Emotion Regulation Measure for Children and Adolescents (POEM-CA)
Authors: Maas Lena , Lohaus Arnold , Rueth Jana-Elisa
Published: May 2024

Abstract: The development of appropriate emotion regulation (ER) strategies is crucial for the healthy development of children and adolescents. Therefore, the assessment of ER skills with economic and yet valid and reliable measures that help to identify ER problems at an early stage is desirable. In this study, the psychometric properties of the short version of the Process-Oriented Emotion Regulation Measure for Children and Adolescents (POEM-CA) were examined in a sample of children and adolescents aged 7 to 18 years in primary (Sample A: N = 362) and secondary school (Sample B: N = 472). The hypothesised factor structure was replicated in both samples and scale and item characteristics were appropriate. The criterion-related validity of the POEM-CA was examined regarding well-being in both samples. As expected, more functional and less dysfunctional ER was associated with higher well-being. Additionally, associations with psychosocial adjustment were tested in Sample B. Functional ER was negatively associated with externalizing problems and positively associated with prosocial behavior. Beyond, dysfunctional ER was associated with more internalizing and externalizing problems. The findings indicate that the POEM-CA is a valid and reliable, but also economic measure that can be useful in research and practice.
Receptivity to Instructional Computer-Based Feedback (RIF)
Authors: Luca Bahr J. , Höft Lars , Meyer Jennifer , Jansen Thorben
Published: May 2024

Abstract: Feedback can play a pivotal role in learning but is only effective when students use it. However, the use of feedback varies greatly from student to student and these differences must be explained. Hence, the construct receptivity to instructional feedback (RIF) was introduced to assess students’ attitudes towards and engagement with human-provided feedback in multiple English-speaking countries. However, RIF scales have not yet been used in computer-based assessment contexts nor in German-speaking countries; their application is thus limited. This study examined an adapted and translated RIF scale using confirmatory factor analysis (CFA) and exploratory structural equation modeling (ESEM). We capitalized on data sets comprising German-speaking university ( N = 464) and secondary school ( N = 1,207) students. The bifactor-CFA model showed a good model fit and revealed a general receptivity factor alongside the four specific factors: experiential attitudes, instrumental attitudes, cognitive engagement, and behavioral engagement with feedback. Scalar measurement invariance allowed for cross-gender and educational level comparisons. The relationships with broader personality characteristics were consistent with other contexts, thus indicating discriminant validity. General receptivity, cognitive engagement, and experiential attitudes positively correlated with GPA, suggesting convergent validity. Results support the stability of the RIF scale’s adaptation and translation. Assessment implications are discussed.
On characteristics of K0 value and shear behaviour of loess using triaxial test
Authors: Liu Xin , Xu Xinyu , Huang Liang , Wei Xiao , Lan Hengxing
Published: May 2024

Compared with conventional soils, such as sand and clay, little knowledge on the coefficient of lateral earth pressure at-rest (K0) has been established for loess in the current literature. This paper presents an experimental investigation on K0 of compacted loess and the associated impacts on undrained shear behaviour. By adopting a K0 consolidation module in the triaxial system, the K0 stress state for loess samples was achieved through a unique feedback control. During the K0 consolidation, the deviatoric stress (q) increases progressively with the premise that the volumetric strain (εv) of the sample equals to the axial strain (εa). The results show that the K0 value of compacted loess is in a range of 0.28 to 0.53, which is dependent on the packing density and the clay content. A distinguishable decrease of K0 was found in the course of K0 consolidation for the loosely compacted loess sample, whereas a similar trend was not observed in the dense sample. In the undrained shear stage, all loess specimens revealed contractive response in the stress path (q-p’) diagram, which can be quantified by a modified collapsibility index (Ic). The index is consistently higher for the K0 consolidated loess samples than for the isotropic ones. The experimental results indicate a strong impact of the initial stress state on the shear behaviour of compacted loess.
Inhibition of NF-kB and COX-2 by andrographolide regulates the progression of cervical cancer by promoting PTEN expression and suppressing PI3K/AKT signalling pathway
Authors: Pasha Akbar , Kumar Kiran , Heena S K , Arnold Emerson I. , Pawar Smita C.
Published: May 2024

In the face of recent advances in Cervical cancer (CC) treatment, therapeutic and surgical procedures for CC management are still inadequate. In the current study for the first time Andrographolide (Andro) has been explored for its multitarget therapeutic efficacy on NF-kB, COX-2, and PI3K/AKT expressions together in CC. The expression levels of NF-kB, COX-2, PI3K and PTEN in the CC patient samples, both at mRNA and protein levels have shown significant association with poor survival and increased tumor aggressiveness. The binding efficacy of Andro was investigated using molecular docking and molecular dynamic simulations, and the protein and ligand complex for NF-kB and COX-2 has shown high binding energy. Andro displayed cytotoxicity by impeding the in-vitro proliferation of CC cells. Andro significantly supressed the NF-kB, COX-2, and PI3K expression and enhanced the expression levels of PTEN at protein levels in-vitro. Andro induced apoptosis in a dose dependent manner and significantly inhibited the migration and invasion of CC cells. Andro exhibited similar activity in-vivo and suppressed the CC tumor growth in xenograft C57BL/6 mice model. The anti-tumor activity of Andro, both in-vitro and in-vivo has shown considerable downregulation of NF-kB and COX-2 and induced apoptosis through impeding the PI3K/AKT signalling pathway. These findings from the above study projects, administration of Andro as an effective alternate safe compound to curtail and impede cervical cancer progression.
Clinical characteristics and optical coherence tomography of concomitant macular hole and rhgematogenous retinal detachment
Authors: Yang Benjamin Chi-Lan , Tsui Mei-Chi , Yang Chung-May , Fu Yu-Teng , Hwang Jiunn-Feng , Lai Tso-Ting , Ho Tzyy-Chang , Yang Chang-Ho , Hsieh Yi-Ting , Chen San-Ni
Published: May 2024

To study the clinical characteristics of macula off rhegmatogenous retinal detachment (RRD) with peripheral causative breaks and concomitant macular hole (RRD+MH). This is a bi-center study. Consecutive eyes of macula off RRD with or without macular hole (MH) were collected. Eyes in these two groups were compared with best corrected visual acuity in logarithm of minimal angle of resolution (logMAR BCVA), the presence of choroidal detachment (CD), proliferative vitreoretinopathy (PVR) and the extent of RRD. In the group of RRD+MH, regression analysis was used to evaluate the correlation of clinical factors and final logMar BCVA. In addition, optical coherence tomography was performed both pre-and post-operatively if possible. There were 40 eyes in the RRD+MH group and 80 eyes in the control group. Eyes with RRD+MH had worse initial and final logMar BCVA (p < 0.001), higher incidence of CD (p < 0.001), PVR and extensive RRD at baseline (p < 0.001). Among the eyes with RRD+MH, final BCVA was correlated with initial BCVA (p < 0.001, CI 0.637 to 0.837), recurrent RRD (p = 0.004, CI − 0.661 to − 0.126), duration of RRD (p = 0.021, CI − 0.576 to − 0.048) and presence of PVR (p = 0.001, CI − 0.131 to − 0.035). The hole closure rate at final follow up is 87.5%.11 of the 17 eyes had preoperative optical coherence tomography (OCT) obtained had ellipsoid zone lining the bottom of MH. CD, PVR and extensive RRD were more commonly observed in RRD+MH. The morphology of MH may suggest the pathogenesis of MH in RRD+MH include mechanism different from that of idiopathic MH.
Computing edge version of metric dimension of certain chemical networks
Authors: Farooq Muhammad Umer , Hussain Muhammad , Jan Ahmed Zubair , Mjaeed Afraz Hussain , Sediqma Mirwais , Amjad Ayesha
Published: May 2024

In the modern digital sphere, graph theory is a significant field of research that has a great deal of significance. It finds widespread application in computer science, robotic directions, and chemistry. Additionally, graph theory is used in robot network localization, computer network problems and the formation of various chemical structures for networks. Moreover, it finds uses in exploring diffusion mechanisms and scheduling aircraft as well. The present research project examines and concentrates on the edge version of metric dimension of the Concealed Non-Kekuléan Benzenoid Hydrocarbon, Polythiophene, Backbone DNA network and Bakelite networks. All the mentioned networks have constant edge metric dimension except Bakelite network, as demonstrated by the results. If we talk about the applications of these networks, Polythiophene are used to treat prion disorders. It is also capable of detecting metal ions. The concept of Bakelite, which finds applications in the jewelry, electrical, cookware, sports, and clock industries, had an impact on the invention of modern polymers. The functions of DNA include information encoding, replication, mutation, and recombination gene expression.
Assessment of intraocular foreign body using high resolution 3D ultrasound imaging
Authors: Minhaz Ahmed Tahseen , Orge Faruk H. , Wilson David L. , Bayat Mahdi
Published: May 2024

Ocular trauma often involves intraocular foreign bodies (IOFBs) that pose challenges in accurate diagnosis due to their size, shape, and material composition. In this study, we proposed a novel whole-eye 3D ophthalmic ultrasound B-scan (3D-UBS) system for automating image acquisition and improved 3D visualization, thereby improving sensitivity for detecting IOFBs. 3D-UBS utilizes 14 MHz Clarius L20 probe, a motorized translation stage, and a surgical microscope for precise placement and movement. The system’s 3D point spread function (PSF) is 0.377 × 0.550 × 0.894 mm3 characterized by the full-width at half-maximum intensity values in the axial, lateral and elevation directions. Digital phantom and ex vivo ocular models were prepared using four types of IOFBs (i.e., plastic, wood, metal, and glass). Ex vivo models were imaged with both 3D-UBS and clinical computed tomography (CT). Image preprocessing was performed on 3D-UBS images to remove uneven illumination and speckle noise. Multiplanar reformatting in 3D-UBS provides optimal plane selection after acquisition, reducing the need for a trained ultrasonographer. 3D-UBS outperforms CT in contrast for wood and plastic, with mean contrast improvement of 2.43 and 1.84 times, respectively. 3D-UBS was able to identify wood and plastic IOFBs larger than 250 µm and 300 in diameter, respectively. CT, with its wider PSF, was only able to detect wood and plastic IOFBs larger than 600 and 550 µm, respectively. Although contrast was higher in CT for metal and glass IOFBs, 3D-UBS provided sufficient contrast to identify those. 3D-UBS provides an easy-to-use, non-expert imaging approach for identifying small IOFBs of different materials and related ocular injuries at the point of care.
The serum tenascin C level is a marker of metabolic disorder-related inflammation affecting pancreatic cancer prognosis
Authors: Sato Katsuhiko , Hikita Hayato , Shigekawa Minoru , Soma Kazumasa , Yamauchi Ryohei , Sung Jihyun , Kato Seiya , Sasaki Yoichi , Kudo Shinnosuke , Fukumoto Kenji , Shirai Kumiko , Murai Kazuhiro , Tahata Yuki , Yoshioka Teppei , Nishio Akira , Saito Yoshinobu , Kodama Takahiro , Sasaki Yutaka , Tatsumi Tomohide , Takehara Tetsuo
Published: May 2024

Obesity is a risk factor for pancreatic cancer development, partly due to the tissue environment of metabolic disorder-related inflammation. We aimed to detect a tissue environment marker triggered by obesity-related metabolic disorders related to pancreatic cancer progression. In murine experiments, Bl6/j mice fed a normal diet (ND) or a high-fat diet (HFD) were orthotopically injected with mPKC1, a murine-derived pancreatic cancer cell line. We used stocked sera from 140 pancreatic cancer patients for analysis and 14 colon polyp patients as a disease control. Compared with ND-fed mice, HFD-fed mice exhibited obesity, larger tumors, and worse prognoses. RNA sequencing of tumors identified tenascin C (TNC) as a candidate obesity-related serum tissue environment marker with elevated expression in tumors of HFD-fed mice. Serum TNC levels were greater in HFD-fed mice than in ND-fed mice. In pancreatic cancer patients, serum TNC levels were greater than those in controls. The TNC-high group had more metabolic disorders and greater CA19-9 levels than did the TNC-low group. There was no relationship between serum TNC levels and disease stage. Among 77 metastatic patients treated with chemotherapy, a high serum TNC concentration was an independent poor prognostic factor. Pancreatic cancer patients with high serum TNC levels experienced progression more rapidly.
Proteinuria and risk of ocular motor cranial nerve palsy: a nationwide population-based study
Authors: Lee Juha , Han Kyungdo , Yoo Juhwan , Park Kyung-Ah , Oh Sei Yeul
Published: May 2024

Understanding the association between dipstick-detected proteinuria and oculomotor cranial nerve palsy (CNP) could have significant implications for understanding the mechanism of CNP development and for developing preventive strategies against CNP development in patients with proteinuria. This study aimed to determine the relationship between dipstick-determined proteinuria and ocular motor CNP using National Sample Cohort (NSC) database from Korea’s National Health Insurance Service (NHIS). A nationwide population-based cohort study was conducted using data from the NSC database of Korea’s NHIS. These data were collected from 2009 to 2018. A one-year time lag was established to prevent a situation in which the causal link was inverted. Participants aged 20 years or more who were diagnosed with proteinuria in 2009 were included. Individuals with specific pre-existing CNP, missing data, and those who were newly diagnosed with CNP or who died within one year of being tested were excluded. The study population was classified into six groups according to the degree of proteinuria (negative, trace, or between 1 + and 4 +) based on the urine dipstick test. A Cox proportional hazard regression analysis was performed to determine the linkage between the degree of proteinuria and ocular motor CNP. A total of 5,807 (0.14% of subjects) with ocular motor CNP were assigned to the ocular motor CNP group and 4,047,205 subjects were assigned to the control group. After full adjustment of comorbidities, hazard ratios (HRs) for 1 + , 2 + , 3 + and 4 + proteinuria groups were 1.449 (95% confidence interval [CI] 1.244–1.687), 2.081 (1.707–2.538), 1.96 (1.322–2.904), and 3.011 (1.507–6.014), respectively, for developing ocular motor CNP compared to the proteinuria-negative group. In subgroup analysis, the HR of patients with proteinuria for the development of ocular motor CNP was higher in the younger age group (less than 40 years) (P = 0.0242) and the group with DM (P = 0.04). Our population-based cohort study demonstrated a significant association between proteinuria and the incidence of CNP, suggesting that urine protein level could be a new clinical marker for predicting the development of CNP.
Habitat type modulates sharp body mass oscillations in cyclic common vole populations
Authors: Olea Pedro P. , de Diego Noelia , García Jesús T. , Viñuela Javier
Published: May 2024

Cyclic rodent populations exhibit pronounced changes in body mass associated with the population cycle phase, long-known as Chitty effect. Although Chitty effect is a common epiphenomenon in both America and Europe, there is still incomplete evidence about the generality of these patterns across the entire range of most species. Moreover, despite decades of research, the underlying factors driving Chitty effect remains poorly understood. Here, we examined the influence of intrinsic and extrinsic factors that may underlie observed patterns in vole size variation in the Iberian common vole Microtus arvalis asturianus. We weighed and measured 2816 adult voles that were captured during 6 trapping periods. Vole numbers and body mass showed strong period- and phase-related variation both in females and males, demonstrating marked Chitty effect in the studied population. Body mass of adult males correlated with body length, evidencing that heavier males are also structurally larger. Statistical models showed that probability of occurrence of large-sized vole (> 37 g) was significantly more likely in reproductive males, during increase and peak phases, and it was modulated by habitat, with crop fields and field margins between crops showing an increased likelihood. We suggest an effect of the habitat on vole body mass mediated by predation.
Effect of hypercholesterolemia on circulating and cardiomyocyte-derived extracellular vesicles
Authors: Kovácsházi Csenger , Hambalkó Szabolcs , Sayour Nabil V. , Gergely Tamás G. , Brenner Gábor B. , Pelyhe Csilla , Kapui Dóra , Weber Bennet Y. , Hültenschmidt Alexander L. , Pállinger Éva , Buzás Edit I. , Zolcsák Ádám , Kiss Bálint , Bozó Tamás , Csányi Csilla , Kósa Nikolett , Kellermayer Miklós , Farkas Róbert , Karvaly Gellért B. , Wynne Kieran , Matallanas David , Ferdinandy Péter , Giricz Zoltán
Published: May 2024

Hypercholesterolemia (HC) induces, propagates and exacerbates cardiovascular diseases via various mechanisms that are yet not properly understood. Extracellular vesicles (EVs) are involved in the pathomechanism of these diseases. To understand how circulating or cardiac-derived EVs could affect myocardial functions, we analyzed the metabolomic profile of circulating EVs, and we performed an in-depth analysis of cardiomyocyte (CM)-derived EVs in HC. Circulating EVs were isolated with Vezics technology from male Wistar rats fed with high-cholesterol or control chow. AC16 human CMs were treated with Remembrane HC supplement and EVs were isolated from cell culture supernatant. The biophysical properties and the protein composition of CM EVs were analyzed. THP1-ASC-GFP cells were treated with CM EVs, and monocyte activation was measured. HC diet reduced the amount of certain phosphatidylcholines in circulating EVs, independently of their plasma level. HC treatment significantly increased EV secretion of CMs and greatly modified CM EV proteome, enriching several proteins involved in tissue remodeling. Regardless of the treatment, CM EVs did not induce the activation of THP1 monocytes. In conclusion, HC strongly affects the metabolome of circulating EVs and dysregulates CM EVs, which might contribute to HC-induced cardiac derangements.
Outcomes from a new modified single needle laparoscopic percutaneous extraperitoneal closure and cut off for pediatric inguinal hernia
Authors: Zeng Defeng , Pu Changsheng , Guo Chunbao , Kong Xiangpan
Published: May 2024

Inguinal hernia is a prevalent surgical condition in pediatric patients. Despite the efficacy of current treatment modalities, a certain recurrence rate still persists. Hence, our objective in this study is to introduce an innovative surgical technique designed to minimize surgical complications. We conducted a retrospective analysis on 809 pediatric cases that underwent laparoscopic repair with our innovative technique for inguinal hernia from June 2020 to June 2022. Demographic information, perioperative details, and postoperative follow-up outcomes were thoroughly assessed. All surgeries were conducted laparoscopically under general anesthesia. The procedure commenced by encircling the hernia sac with two sutures under laparoscopic guidance. Subsequently, the sac was exteriorized from the body using the two sutures, followed by ligation and excision of the hernia sac. The research findings demonstrate that the duration of unilateral and bilateral procedures was recorded as 15.9 ± 4.8 and 21.7 ± 3.9 min, respectively. Incision infection occurred in 7 patients (0.87%), and Male Complicated Inguinal Hernia (MCIH) was observed in 2 patients (0.23%). Notably, there were no occurrences of iatrogenic cryptorchidism, testicular atrophy, or recurrence (0%) during the follow-up period. In conclusion, our novel modification shows a notable reduction in postoperative recurrence rates and alleviates the impact of the procedure on the positioning of the testis or uterus. This modified technique is both safe and valuable, thus warranting broader adoption and promotion.
The geographic distribution, and the biotic and abiotic predictors of select zoonotic pathogen detections in Canadian polar bears
Authors: Tschritter Christina M. , van Coeverden de Groot Peter , Branigan Marsha , Dyck Markus , Sun Zhengxin , Jenkins Emily , Buhler Kayla , Lougheed Stephen C.
Published: May 2024

Increasing Arctic temperatures are facilitating the northward expansion of more southerly hosts, vectors, and pathogens, exposing naïve populations to pathogens not typical at northern latitudes. To understand such rapidly changing host–pathogen dynamics, we need sensitive and robust surveillance tools. Here, we use a novel multiplexed magnetic-capture and droplet digital PCR (ddPCR) tool to assess a sentinel Arctic species, the polar bear (Ursus maritimus; n = 68), for the presence of five zoonotic pathogens (Erysipelothrix rhusiopathiae, Francisella tularensis, Mycobacterium tuberculosis complex, Toxoplasma gondii and Trichinella spp.), and observe associations between pathogen presence and biotic and abiotic predictors. We made two novel detections: the first detection of a Mycobacterium tuberculosis complex member in Arctic wildlife and the first of E. rhusiopathiae in a polar bear. We found a prevalence of 37% for E. rhusiopathiae, 16% for F. tularensis, 29% for Mycobacterium tuberculosis complex, 18% for T. gondii, and 75% for Trichinella spp. We also identify associations with bear age (Trichinella spp.), harvest season (F. tularensis and MTBC), and human settlements (E. rhusiopathiae, F. tularensis, MTBC, and Trichinella spp.). We demonstrate that monitoring a sentinel species, the polar bear, could be a powerful tool in disease surveillance and highlight the need to better characterize pathogen distributions and diversity in the Arctic.
Prognostic significance of CT-determined sarcopenia in older patients with advanced squamous cell lung cancer treated with programmed death-1 inhibitors
Authors: Ying Lin , Xu Liqian , Yang Ji , Zhang Qin
Published: May 2024

Sarcopenia has been associated with higher toxicity induced by anti-cancer treatments and shorter survival in patients with squamous cell lung carcinoma (SqCLC). Over the past few decades, immune checkpoint inhibitors (ICIs) significantly improves the prognosis. However, few clinical studies explored the effectiveness of immunotherapy in the elderly population. Here, we performed a retrospective analysis to determine the prognostic role of sarcopenia in older patients with SqCLC receiving ICIs. We retrospectively assessed SqCLC patients who were treated with PD-1 inhibitors and all patients were at least 70 years old. Pre-treatment sarcopenic status was determined by analyzing L3 skeletal muscle index (SMI) with chest CT. Progression-free survival (PFS), disease-specific survival (DSS) and overall survival (OS) were estimated using the Kaplan–Meier method, and the differences in survival were compared using the log-rank test. Among 130 male SqCLC patients, 93 had sarcopenia. Patients with sarcopenia were older and had a lower body mass index (BMI). Over an average follow-up of 20.8 months, 92 patients died. For all 130 patients, the mean OS was 13.3 months. Patients with sarcopenia had a significantly shorter OS and PFS than those without sarcopenia (OS, 12.4 ± 5.2 months vs. 15.5 ± 10.5 months, P = 0.028; PFS, 6.4 ± 2.9 months vs. 7.7 ± 4.2 months; P = 0.035). Multivariable analysis showed that sarcopenia was an independent prognostic factor for shorter OS and PFS. CT-determined sarcopenia is an independent prognostic factor for older patients with SqCLC receiving ICIs.