bluuebunny/crossref_metadata_2025 huggingface dataset:
The Accuracy of Self-Reported Body Weight Is High but Dependent on Recent Weight Change and Negative Affect in Teenage Girls
Authors: Koebnick Corinna , Saksvig Brit , Li Xia , Sidell Margo , Wu Tong Tong , Young Deborah R.
Published: November 2020

Background: Research studies often rely on self-reported weight to calculate body mass index. The present study investigated how the accuracy of self-reported body weight in adolescent girls is affected by overweight/obesity, race/ethnicity, and mental health factors. Methods: In a cohort of girls who participated in the Trial of Activity for Adolescent Girls at ages 11 and 17 (n = 588), self-reported and measured weight were compared, and linear regression models were fitted to model the over- or underreporting. The Center for Epidemiological Studies-Depression Scale (CES-D) was used to calculate depressive symptom subscales for negative affect, anhedonia and somatic symptoms. Results: Allowing 3% difference between self-reported and measured weight for the correct reporting of body weight, 59.2% of girls reported their weight correctly, 30.3% underreported (−5.8 ± 4.8 kg), and 10.5% overreported (4.3 ± 3.5 kg). The average difference between self-reported and measured body weight was −1.5 ± 4.3 kg (p < 0.001). Factors for misreporting body weight were overweight (β ± SE − 2.60 ± 0.66%), obesity (β ± SE − 2.41 ± 0.71%), weight change between ages 11 and 17 (β ± SE − 0.35 ± 0.04% for each kg), height change between ages 11 and 17 (β ± SE 0.29 ± 0.10% for each cm), and negative affect (β ± SE − 0.18 ± 0.08% for each score unit). Conclusions: The difference between self-reported and measured body weight in adolescent girls is relatively small. However, the accuracy of self-reported body weight may be lower in girls with overweight or obesity, recent weight and height change, and higher negative affect.
Work Engagement among Prison Officers. The Role of Individual and Organizational Factors in the Polish and Indonesian Penitentiary Systems
Authors: Piotrowski Andrzej , Sygit-Kowalkowska Ewa , Hamzah Imaduddin
Published: November 2020

The literature on work engagement among prison officers (POs) remains rather scarce, and there are no analyses on the factors determining this phenomenon. The current study aimed to examine the relationships between work engagement, subjective well-being, coping strategies, and organizational factors utilizing the Utrecht Work Engagement Scale (UWES-9), the Coping Orientation to Problems Experienced (COPE), and Cantril’s Ladder of Health Scale (CLHS), and involving 312 POs from Poland and 467 POs from Indonesia. Results showed a statistically significant relationship between active coping and work engagement in both groups. Subjective well-being was moderately related to work engagement among Polish POs. Mean work engagement and subjective well-being scores were higher among Indonesian POs. The analyses showed a significant indirect effect of subjective well-being for the relationship between penitentiary unit type, active coping, as well as avoidant behaviors and work engagement in the Polish group. Closed prison officers more often declared higher subjective well-being. Work engagement is a complex psychological phenomenon. There exists a justified need for the analyses to consider personal determinants (e.g., coping strategies) as well as organizational factors related to the POs’ work environment. The literature presents a broad picture of the benefits of studying this phenomenon.
Brief Report on Double-Chamber Syringes Patents and Implications for Infusion Therapy Safety and Efficiency
Authors: Sousa Liliana B. , Santos-Costa Paulo , Marques Inês A. , Cruz Arménio , Salgueiro-Oliveira Anabela , Parreira Pedro
Published: November 2020

This review aimed to map the existing patents of double-chamber syringes that can be used for intravenous drug administration and catheter flush. A search was conducted in the Google patents database for records published prior to 28 October 2020, using several search terms related to double-chamber syringes (DCS). Study eligibility and data extraction were performed by two independent reviewers. Of the initial 26,110 patents found, 24 were included in this review. The 24 DCS that were found display two or more independent chambers that allow for the administration of multiple solutions. While some of the DCS have designated one of the chambers as the flushing chamber, most patents only allow for the sequential use of the flushing chamber after intravenous drug administration. Most DCS were developed for drug reconstitution, usually with a freeze-dried drug in one chamber. Some patents were designed for safety purposes, with a parallel post-injection safety sheath chamber for enclosing a sharpened needle tip. None of the DCS found allow for a pre- and post-intravenous drug administration flush. Given the current standards of care in infusion therapy, future devices must allow for the sequential use of the flushing chamber to promote a pre-administration patency assessment and a post-administration device flush.
Primitive Reflex Activity in Relation to the Sensory Profile in Healthy Preschool Children
Authors: Pecuch Anna , Gieysztor Ewa , Telenga Marlena , Wolańska Ewelina , Kowal Mateusz , Paprocka-Borowicz Małgorzata
Published: November 2020

The presence of active primitive reflexes (APRs) in healthy preschool children can be an expression of immaturity in the functioning of the nervous system. Their trace presence may not significantly affect the quality of child functioning. They may also undergo spontaneous and complete integration within the stages of child development. However, a higher level of active reflexes and their significant number can disturb sensory-motor development and lead to additional problems in a child’s motor activities, social life, and education. The main purpose of this study was to examine the types of sensory disorders noticed by parents of children, if any, that accompany the presence of active primitive reflexes. The study was conducted in a group of 44 preschool children (aged 4–6 years). The sensory profile of children was determined using Child Sensory Profile Cards, and Sally Goddard-Blythe tests were used to measure their primitive reflexes. The coefficient of determination (R-squared) indicated that the level of reflex activity was most strongly associated with sensory disorders such as dyspraxia, sensory-vestibular disorders, and postural disorders, at a level of p < 0.005. The obtained research results show that the examination of non-integrated reflexes might be a screening tool for children of preschool age. Knowledge of the subject of reflexes and their impact on sensory-motor functions may contribute to more accurate diagnoses of the causes of problems and higher effectiveness of possible therapy.
Effect of Glenohumeral Internal Rotation Deficit on Shoulder in Baseball Pitchers during Fastball Pitching
Authors: Lin Hwai-Ting , Lin Yu-Chuan , Chou You-Li , Wu Hung-Chien , Wang Rong-Tyai , Chou Paul Pei-His
Published: November 2020

Previous studies have reported that pitchers with glenohumeral internal rotation deficit (GIRD) may increase the risk of shoulder injury. However, limited information is available regarding the specific effects of GIRD in baseball pitching. The purpose of this study was to investigate whether baseball pitchers with GIRD change their pitching mechanism. Fifteen baseball pitchers with GIRD and 15 pitchers without GIRD were recruited from university or senior high-school teams. A three-dimensional motion analysis system (Eagle System, Motion Analysis Corporation, Santa Rosa, CA, USA) was used to capture the pitching motion while performing fastball pitches. The kinematics and kinetics of the throwing shoulder and trunk were analyzed based on motion captured data. The Mann–Whitney U test was used to test the differences of the analyzed parameters between two groups. At the instant of ball release, the GIRD group showed lower shoulder external rotation and trunk rotation, and larger shoulder horizontal adduction. In addition, the GIRD group exhibited a significantly larger shoulder inferior force in the cocking and acceleration phase, and a significantly larger internal rotation torque in the acceleration phase. The present results suggested that pitchers with GIRD need stretch training to enlarge joint range of motion, and to improve trunk strength and flexibility to alleviate potential problems associated with pitching in GIRD pitchers.
Health Care Workers’ Perspectives of the Influences of Disrespectful Maternity Care in Rural Kenya
Authors: Lusambili Adelaide , Wisofschi Stefania , Shumba Constance , Obure Jerim , Mulama Kennedy , Nyaga Lucy , Wade Terrance J. , Temmerman Marleen
Published: November 2020

While disrespectful treatment of pregnant women attending health care facilities occurs globally, it is more prevalent in low-resource countries. In Kenya, a large body of research studied disrespectful maternity care (DMC) from the perspective of the service users. This paper examines the perspective of health care workers (HCWs) on factors that influence DMC experienced by pregnant women at health care facilities in rural Kisii and Kilifi counties in Kenya. We conducted 24 in-depth interviews with health care workers (HCWs) in these two sites. Data were analyzed deductively and inductively using NVIVO 12. Findings from HCWs reflective narratives identified four areas connected to the delivery of disrespectful care, including poor infrastructure, understaffing, service users’ sociocultural beliefs, and health care workers’ attitudes toward marginalized women. Investments are needed to address health system influences on DMC, including poor health infrastructure and understaffing. Additionally, it is important to reduce cultural barriers through training on HCWs’ interpersonal communication skills. Further, strategies are needed to affect positive behavior changes among HCWs directed at addressing the stigma and discrimination of pregnant women due to socioeconomic standing. To develop evidence-informed strategies to address DMC, a holistic understanding of the factors associated with pregnant women’s poor experiences of facility-based maternity care is needed. This may best be achieved through an intersectional approach to address DMC by identifying systemic, cultural, and socioeconomic inequities, as well as the structural and policy features that contribute and determine peoples’ behaviors and choices.
Expectancy-Value Model Related to Physical Activity Behaviors in Chilean and Spanish Adolescents
Authors: Gallardo Laura O. , Abarca-Sos Alberto , Doña Alberto Moreno
Published: November 2020

The purpose of the study is to comparatively test the expectancy-value model in Chilean and Spanish samples. The model proposes: a social world (composed of social support, physical activity teasing, and weight teasing), expectancy (composed of perceived competence and appearance), task values (composed of enjoyment and stress) to predict physical activity and intention to be physically active. Participants were 497 (Chilean) and 1365 (Spanish) adolescents. Structural equation models and multi-group modelling were used. All the models presented adequate fit to the data. The results show that physical activity teasing is a contextual and essential variable; perceived competence and enjoyment influenced physical activity and intentions to be physically active; some differences appeared in the prediction of physical activity and intentions to be physically active when the multi-group model was run. Culturally tailored interventions are key to improving physical activity (PA) behaviors.
Increased Oxidative Stress Underlies Abnormal Pain Threshold in a Normoglycemic Japanese Population
Authors: Osonoi Sho , Mizukami Hiroki , Itabashi Chieko , Wada Kanichiro , Kudoh Kazuhiro , Igawa Akiko , Ogasawara Saori , Ishibashi Yasuyuki , Daimon Makoto , Yagihashi Soroku , Nakaji Shigeyuki
Published: November 2020

Normal-high HbA1c levels are a risk factor for attenuated pain sensation in normoglycemic subjects. It is unclear, however, what mechanisms underlie the pathogenesis of attenuated pain sensation in such a population. We, therefore, explored the relationship between oxidative stress (OS) and pain sensation in a rural Japanese population. A population-based study of 894 individuals (average age 53.8 ± 0.5 years) and 55 subjects with impaired fasting glucose (IFG) were enrolled in this study. Individuals with diabetes were excluded. Relationships between pain threshold induced by intraepidermal electrical stimulation (PINT) and clinico-hematological parameters associated with OS were evaluated. Univariate linear regression analyses revealed age, BMI, HbA1c, the OS biomarker urine 8-hydroxy-2′-deoxyguanosine (8-OHdG), systolic blood pressure, and decreased Achilles tendon reflex on the PINT scores. Adjustments for age, gender, and multiple clinical measures confirmed a positive correlation between PINT scores and urine 8-OHdG (β = 0.09, p < 0.01). Urine 8-OHdG correlated positively with higher HbA1c levels and age in the normoglycemic population. Unlike in the normoglycemic population, both inflammation and OS were correlated with elevated PINT scores in IFG subjects. OS may be a major contributing factor to elevated PINT scores in a healthy Japanese population.
Molecular Mechanisms Underlying Muscle Wasting in Huntington’s Disease
Authors: Bozzi Manuela , Sciandra Francesca
Published: November 2020

Huntington’s disease (HD) is an autosomal dominant neurodegenerative disorder caused by pathogenic expansions of the triplet cytosine-adenosine-guanosine (CAG) within the Huntingtin gene. These expansions lead to a prolongation of the poly-glutamine stretch at the N-terminus of Huntingtin causing protein misfolding and aggregation. Huntingtin and its pathological variants are widely expressed, but the central nervous system is mainly affected, as proved by the wide spectrum of neurological symptoms, including behavioral anomalies, cognitive decline and motor disorders. Other hallmarks of HD are loss of body weight and muscle atrophy. This review highlights some key elements that likely provide a major contribution to muscle atrophy, namely, alteration of the transcriptional processes, mitochondrial dysfunction, which is strictly correlated to loss of energy homeostasis, inflammation, apoptosis and defects in the processes responsible for the protein quality control. The improvement of muscular symptoms has proven to slow the disease progression and extend the life span of animal models of HD, underlining the importance of a deep comprehension of the molecular mechanisms driving deterioration of muscular tissue.
The Bacterial Anti-Adhesive Activity of Double-Etched Titanium (DAE) as a Dental Implant Surface
Authors: Petrini Morena , Giuliani Alessandra , Di Campli Emanuela , Di Lodovico Silvia , Iezzi Giovanna , Piattelli Adriano , D’Ercole Simonetta
Published: November 2020

This work aimed to compare the capability of Streptococcus oralis to adhere to a novel surface, double-etched titanium (DAE), in respect to machined and single-etched titanium. The secondary outcome was to establish which topographical features could affect the interaction between the implant surface and bacteria. The samples’ superficial features were characterized using scanning electron microscopy (SEM) and energy dispersive x-ray spectrometry (EDS), and the wetting properties were tested through sessile methods. The novel surface, the double-etched titanium (DAE), was also analyzed with atomic force microscopy (AFM). S. oralis was inoculated on discs previously incubated in saliva, and then the colony-forming units (CFUs), biomass, and cellular viability were measured at 24 and 48h. SEM observation showed that DAE was characterized by higher porosity and Oxygen (%) in the superficial layer and the measurement of the wetting properties showed higher hydrophilicity. AFM confirmed the presence of a higher superficial nano-roughness. Microbiological analysis showed that DAE discs, coated by pellicle’s proteins, were characterized by significantly lower CFUs at 24 and 48 h with respect to the other two groups. In particular, a significant inverse relationship was shown between the CFUs at 48 h and the values of the wetted area and a direct correlation with the water contact angle. The biomass at 24 h was slightly lower on DAE, but results were not significant concerning the other groups, both at 24 and 48 h. The DAE treatment not only modifies the superficial topography and increased hydrophilicity, but it also increases the Oxygen percentage in the superficial layer, which could contribute to the inhibition of S. oralis adhesion. DAE can be considered a promising treatment for titanium implants to counteract a colonization pioneer microorganism, such as S. oralis.
Genome-Wide Identification of the Tify Gene Family and Their Expression Profiles in Response to Biotic and Abiotic Stresses in Tea Plants (Camellia sinensis)
Authors: Zhang Xin , Ran Wei , Zhang Jin , Ye Meng , Lin Songbo , Li Xiwang , Sultana Riffat , Sun Xiaoling
Published: November 2020

The TIFY family is a plant-specific gene family that is involved in regulating a variety of plant processes, including developmental and defense responses. The chromosome-level genome of the tea plant (Camellia sinensis) has recently been released, but a comprehensive view of the TIFY family in C. sinensis (the CsTIFY genes) is lacking. The current study performed an extensive genome-wide identification of CsTIFY genes. The phylogenetics, chromosome location, exon/intron structure, and conserved domains of these genes were analyzed to characterize the members of the CsTIFY family. The expression profiles of the CsTIFY genes in four organs were analyzed, and they showed different spatial expression patterns. All CsJAZ genes were observed to be induced by jasmonate acid (JA) and exhibited different responses to abiotic and biotic stresses. Six of seven CsJAZ genes (CsJAZ1, CsJAZ2, CsJAZ3, CsJAZ4, CsJAZ7, and CsJAZ8) were upregulated by mechanical wounding and infestation with the tea geometrid (Ectropis obliqua), while infection with tea anthracnose (Colletotrichum camelliae) primarily upregulated the expression levels of CsJAZ1 and CsJAZ10. In addition, CsJAZs were observed to interact with CsMYC2 and AtMYC2. Therefore, the results of this study may contribute to the functional characterization of the CsTIFY genes, especially the members of the JAZ subfamily, as regulators of the JA-mediated defense response in tea plant.
TIM-3 Genetic Variants Are Associated with Altered Clinical Outcome and Susceptibility to Gram-Positive Infections in Patients with Sepsis
Authors: Mewes Caspar , Alexander Tessa , Büttner Benedikt , Hinz José , Alpert Ayelet , Popov Aron-F. , Ghadimi Michael , Beißbarth Tim , Tzvetkov Mladen , Grade Marian , Quintel Michael , Bergmann Ingo , Mansur Ashham
Published: November 2020

Background: Previous studies have reported the fundamental role of immunoregulatory proteins in the clinical phenotype and outcome of sepsis. This study investigated two functional single nucleotide polymorphisms (SNPs) of T cell immunoglobulin and mucin domain-containing protein 3 (TIM-3), which has a negative stimulatory function in the T cell immune response. Methods: Patients with sepsis (n = 712) were prospectively enrolled from three intensive care units (ICUs) at the University Medical Center Goettingen since 2012. All patients were genotyped for the TIM-3 SNPs rs1036199 and rs10515746. The primary outcome was 28-day mortality. Disease severity and microbiological findings were secondary endpoints. Results: Kaplan–Meier survival analysis demonstrated a significantly lower 28-day mortality for TIM-3 rs1036199 AA homozygous patients compared to C-allele carriers (18% vs. 27%, p = 0.0099) and TIM-3 rs10515746 CC homozygous patients compared to A-allele carriers (18% vs. 26%, p = 0.0202). The TIM-3 rs1036199 AA genotype and rs10515746 CC genotype remained significant predictors for 28-day mortality in the multivariate Cox regression analysis after adjustment for relevant confounders (adjusted hazard ratios: 0.67 and 0.70). Additionally, patients carrying the rs1036199 AA genotype presented more Gram-positive and Staphylococcus epidermidis infections, and rs10515746 CC homozygotes presented more Staphylococcus epidermidis infections. Conclusion: The studied TIM-3 genetic variants are associated with altered 28-day mortality and susceptibility to Gram-positive infections in sepsis.
New Model for Stacking Monomers in Filamentous Actin from Skeletal Muscles of Oryctolagus cuniculus
Authors: Glyakina Anna V. , Surin Alexey K. , Grishin Sergei Yu. , Selivanova Olga M. , Suvorina Mariya Yu. , Bobyleva Liya G. , Vikhlyantsev Ivan M. , Galzitskaya Oxana V.
Published: November 2020

To date, some scientific evidence (limited proteolysis, mass spectrometry analysis, electron microscopy (EM)) has accumulated, which indicates that the generally accepted model of double-stranded of filamentous actin (F-actin) organization in eukaryotic cells is not the only one. This entails an ambiguous understanding of many of the key cellular processes in which F-actin is involved. For a detailed understanding of the mechanism of F-actin assembly and actin interaction with its partners, it is necessary to take into account the polymorphism of the structural organization of F-actin at the molecular level. Using electron microscopy, limited proteolysis, mass spectrometry, X-ray diffraction, and structural modeling we demonstrated that F-actin presented in the EM images has no double-stranded organization, the regions of protease resistance are accessible for action of proteases in F-actin models. Based on all data, a new spatial model of filamentous actin is proposed, and the F-actin polymorphism is discussed.
LINC00973 Induces Proliferation Arrest of Drug-Treated Cancer Cells by Preventing p21 Degradation
Authors: Karpov Dmitry S. , Spirin Pavel V. , Zheltukhin Andrey O. , Tutyaeva Vera V. , Zinovieva Olga L. , Grineva Evgenia N. , Matrosova Vera A. , Krasnov George S. , Snezhkina Anastasiya V. , Kudryavtseva Anna V. , Prassolov Vladimir S. , Mashkova Tamara D. , Lisitsyn Nikolai A.
Published: November 2020

Overcoming drug resistance of cancer cells is the major challenge in molecular oncology. Here, we demonstrate that long non-coding RNA LINC00973 is up-regulated in normal and cancer cells of different origins upon treatment with different chemotherapeutics. Bioinformatics analysis shows that this is a consequence of DNA damage response pathway activation or mitotic arrest. Knockdown of LINC0973 decreases p21 levels, activates cellular proliferation of cancer cells, and suppresses apoptosis of drug-treated cells. We have found that LINC00973 strongly increases p21 protein content, possibly by blocking its degradation. Besides, we have found that ectopic over-expression of LINC00973 inhibits formation of the pro-survival p53-Ser15-P isoform, which preserves chromosome integrity. These results might open a new approach to the development of more efficient anti-cancer drugs.
The Adrenal Medulla Modulates Mechanical Allodynia in a Rat Model of Neuropathic Pain
Authors: Arribas-Blázquez Marina , Olivos-Oré Luis Alcides , Barahona María Victoria , Wojnicz Aneta , De Pascual Ricardo , Sánchez de la Muela Mercedes , García Antonio G. , Artalejo Antonio R.
Published: November 2020

We have investigated whether the stress response mediated by the adrenal medulla in rats subjected to chronic constriction injury of the sciatic nerve (CCI) modulates their nocifensive behavior. Treatment with SK29661 (300 mg/kg; intraperitoneal (I.P.)), a selective inhibitor of phenylethanolamine N-methyltransferase (PNMT) that converts noradrenaline (NA) into adrenaline (A), fully reverted mechanical allodynia in the injured hind paw without affecting mechanical sensitivity in the contralateral paw. The effect was fast and reversible and was associated with a decrease in the A to NA ratio (A/NA) in the adrenal gland and circulating blood, an A/NA that was elevated by CCI. 1,2,3,4-tetrahydroisoquinoline-7-sulfonamide (SKF29661) did not affect exocytosis evoked by Ca2+ entry as well as major ionic conductances (voltage-gated Na+, Ca2+, and K+ channels, nicotinic acetylcholine receptors) involved in stimulus-secretion coupling in chromaffin cells, suggesting that it acted by changing the relative content of the two adrenal catecholamines. Denervation of the adrenal medulla by surgical splanchnectomy attenuated mechanical allodynia in neuropathic animals, hence confirming the involvement of the adrenal medulla in the pathophysiology of the CCI model. Inhibition of PNMT appears to be an effective and probably safe way to modulate adrenal medulla activity and, in turn, to alleviate pain secondary to the injury of a peripheral nerve.
Chitosan Oligosaccharides Suppress Nuclear Factor-Kappa B Activation and Ameliorate Experimental Autoimmune Uveoretinitis in Mice
Authors: Hsu Sheng-Min , Yang Chang-Hao , Tsai Hsien-Yang , Lin Chia-Jhen , Fang Yi-Hsuan , Shieh Chi-Chang , Chen Shun-Hua
Published: November 2020

We investigated the therapeutic potential and mechanism of chitosan oligosaccharides (COS) for experimental autoimmune uveoretinitis (EAU) in mice. EAU was induced in C57/BL6 mice by injection of human interphotoreceptor retinoid-binding protein (IRBP) peptides. At the same time, a high or low dose (20 or 10 mg/kg) of COS or phosphate-buffered saline (PBS) was given to mice daily after EAU induction. We found that mouse EAU is ameliorated by the high-dose COS treatment when compared with PBS treatment. In the retinas of high-dose COS-treated mice, the nuclear translocation of NF-κB subunit (p65) was suppressed, and the expression of several key EAU inflammatory mediators, IFN-γ, TNF-α, IL-1α, IL-4, IL-5, IL-6, IL-10, IL-17 and MCP-1 was lowered. These results suggest that COS may be a potential treatment for posterior uveitis.
Skeletal Phenotypes Due to Abnormalities in Mitochondrial Protein Homeostasis and Import
Authors: Zhao Tian , Goedhart Caitlin , Pfeffer Gerald , Greenway Steven C , Lines Matthew , Khan Aneal , Innes A Micheil , Shutt Timothy E
Published: November 2020

Mitochondrial disease represents a collection of rare genetic disorders caused by mitochondrial dysfunction. These disorders can be quite complex and heterogeneous, and it is recognized that mitochondrial disease can affect any tissue at any age. The reasons for this variability are not well understood. In this review, we develop and expand a subset of mitochondrial diseases including predominantly skeletal phenotypes. Understanding how impairment ofdiverse mitochondrial functions leads to a skeletal phenotype will help diagnose and treat patients with mitochondrial disease and provide additional insight into the growing list of human pathologies associated with mitochondrial dysfunction. The underlying disease genes encode factors involved in various aspects of mitochondrial protein homeostasis, including proteases and chaperones, mitochondrial protein import machinery, mediators of inner mitochondrial membrane lipid homeostasis, and aminoacylation of mitochondrial tRNAs required for translation. We further discuss a complex of frequently associated phenotypes (short stature, cataracts, and cardiomyopathy) potentially explained by alterations to steroidogenesis, a process regulated by mitochondria. Together, these observations provide novel insight into the consequences of impaired mitochondrial protein homeostasis.
Mass Spectrometry-Based Solid Phase Peptide Reaction Assay for Detecting Allergenicity Using an Immobilized Peptide-Conjugating Photo-Cleavable Linker
Authors: Miyazaki Hiroshi , Samejima Yasutaka , Iwata Kazuya , Minamino Yuuki , Hikida Shinya , Ariumi Hideto , Ikeda Hidefumi , Hamada Yoshio , Yamashita Kunihiko , Usui Kenji
Published: November 2020

The biological process of skin sensitization depends on the ability of a sensitizer to modify endogenous proteins. A direct peptide reactivity assay (DPRA), based on the biological process of skin sensitization, was developed as an alternative to controversial animal experiments. Although DPRA has been endorsed by industries and is internationally accepted as promising, it has several drawbacks, such as incompatibility with hydrophobic chemicals, inability to perform detailed reaction analysis, and ability to evaluate only single components. Here, we demonstrated that sensitizers and peptide adducts can be easily identified using a mass spectrometry-based solid-phase peptide reaction assay (M-SPRA). We synthesized peptides with a photo-cleavable linker immobilized on resins. We showed the potential of M-SPRA in predicting skin sensitization by measuring the peptide adducts that were selectively eluted from the resin after cleaving the linker post-reaction. M-SPRA provides more detailed information regarding chemical reactivity and accurate assessment of test samples, including mixtures. M-SPRA may be helpful for understanding the binding mechanism of sensitizers (toxicology), which may assist in further refining reactivity assays and aiding in the interpretation of reactivity data.
Genetic Variants of Lipoprotein Lipase and Regulatory Factors Associated with Alzheimer’s Disease Risk
Authors: D. Bruce Kimberley , Tang Maoping , Reigan Philip , H. Eckel Robert
Published: November 2020

Lipoprotein lipase (LPL) is a key enzyme in lipid and lipoprotein metabolism. The canonical role of LPL involves the hydrolysis of triglyceride-rich lipoproteins for the provision of FFAs to metabolic tissues. However, LPL may also contribute to lipoprotein uptake by acting as a molecular bridge between lipoproteins and cell surface receptors. Recent studies have shown that LPL is abundantly expressed in the brain and predominantly expressed in the macrophages and microglia of the human and murine brain. Moreover, recent findings suggest that LPL plays a direct role in microglial function, metabolism, and phagocytosis of extracellular factors such as amyloid- beta (Aβ). Although the precise function of LPL in the brain remains to be determined, several studies have implicated LPL variants in Alzheimer’s disease (AD) risk. For example, while mutations shown to have a deleterious effect on LPL function and expression (e.g., N291S, HindIII, and PvuII) have been associated with increased AD risk, a mutation associated with increased bridging function (S447X) may be protective against AD. Recent studies have also shown that genetic variants in endogenous LPL activators (ApoC-II) and inhibitors (ApoC-III) can increase and decrease AD risk, respectively, consistent with the notion that LPL may play a protective role in AD pathogenesis. Here, we review recent advances in our understanding of LPL structure and function, which largely point to a protective role of functional LPL in AD neuropathogenesis.
Micro-Structures Produced by Crystal Growth from Located Nuclei and Their Transfer Aiming at Functional Surfaces
Authors: Moronuki Nobuyuki , Serizawa Renato
Published: November 2020

Hydrothermal processes can produce regular micro-/nano-structures easily; but their placement or position is difficult to control, and the obtainable structures tend to be random. For controlling the crystal growth, two types of definite and regular structures were obtained. The first ones were ZnO urchin-like structures synthesized from located ZnO particles as the nuclei. These structures were found to work as gas sensors utilizing a wide surface area. The second one was a vertically aligned TiO2 nanorod array synthesized on a fluorine-doped tin oxide substrate that has a similar lattice constant to rutile TiO2. Super-hydrophobicity after ultraviolet irradiation was then examined. Finally, the synthesized TiO2 array was peeled off and transferred onto a resin sheet. We determined that the substrate could be subjected to repeated hydrothermal synthesis, thereby demonstrating the reusability of the substrate. These results demonstrate the applicability of these processes for industrial applications.
Insights into the Current Trends in the Utilization of Bacteria for Microbially Induced Calcium Carbonate Precipitation
Authors: Chuo Sing Chuong , Mohamed Sarajul Fikri , Mohd Setapar Siti Hamidah , Ahmad Akil , Jawaid Mohammad , Wani Waseem A. , Yaqoob Asim Ali , Mohamad Ibrahim Mohamad Nasir
Published: November 2020

Nowadays, microbially induced calcium carbonate precipitation (MICP) has received great attention for its potential in construction and geotechnical applications. This technique has been used in biocementation of sand, consolidation of soil, production of self-healing concrete or mortar, and removal of heavy metal ions from water. The products of MICP often have enhanced strength, durability, and self-healing ability. Utilization of the MICP technique can also increase sustainability, especially in the construction industry where a huge portion of the materials used is not sustainable. The presence of bacteria is essential for MICP to occur. Bacteria promote the conversion of suitable compounds into carbonate ions, change the microenvironment to favor precipitation of calcium carbonate, and act as precipitation sites for calcium carbonate crystals. Many bacteria have been discovered and tested for MICP potential. This paper reviews the bacteria used for MICP in some of the most recent studies. Bacteria that can cause MICP include ureolytic bacteria, non-ureolytic bacteria, cyanobacteria, nitrate reducing bacteria, and sulfate reducing bacteria. The most studied bacterium for MICP over the years is Sporosarcina pasteurii. Other bacteria from Bacillus species are also frequently investigated. Several factors that affect MICP performance are bacterial strain, bacterial concentration, nutrient concentration, calcium source concentration, addition of other substances, and methods to distribute bacteria. Several suggestions for future studies such as CO2 sequestration through MICP, cost reduction by using plant or animal wastes as media, and genetic modification of bacteria to enhance MICP have been put forward.
Special Issue: “Non-Destructive Testing of Structures”
Authors: Rucka Magdalena
Published: November 2020

The Special Issue “Non-Destructive Testing of Structures” has been proposed to present recent developments in the field of diagnostics of structural materials and components in civil and mechanical engineering. The papers highlighted in this editorial concern various aspects of non-invasive diagnostics, including such topics as condition assessments of civil and mechanical structures and connections of structural elements, the inspection of cultural heritage monuments, the testing of structural materials, structural health monitoring systems, the integration of non-destructive testing methods, advanced signal processing for the non-destructive testing of structures (NDT), damage detection and damage imaging, as well as modeling and numerical analyses for supporting structural health monitoring (SHM) systems.
The Effect of Polarity and Hydrostatic Pressure on Operational Characteristics of Rutile Electrode in Underwater Welding
Authors: Moreno-Uribe Andrés M. , Bracarense Alexandre Q. , Pessoa Ezequiel C. P.
Published: November 2020

In order to provide a better understanding of the phenomena that define the weld bead penetration and melting rate of consumables in underwater welding, welds were developed with a rutile electrode in air welding conditions and at the simulated depths of 5 and 10 m with the use of a hyperbaric chamber and a gravity feeding system. In this way, voltage and current signals were acquired. Data processing involved the welding voltage, determination of the sum of the anodic and cathodic drops, calculation of the short-circuit factor, and determination of the melting rate. Cross-sectional samples were also taken from the weld bead to assess bead geometry. As a result, the collected data show that the generation of energy in the arc–electrode connection in direct polarity (direct current electrode negative-DCEN) is affected by the hydrostatic pressure, causing a loss of fusion efficiency, a drop of operating voltage, decreased arc length, and increased number of short-circuit events. The combination of these characteristics kept the weld bead geometry unchanged, compared to dry weld conditions. With the positive electrode (direct current electrode positive-DCEP), radial losses were derived from greater arc lengths resulting from increasing hydrostatic pressure, which led to a decrease in weld penetration.
Investigation of Kenaf Paper in the Presence of PVA for Transformers Application
Authors: Umair Muhammad , Azis Norhafiz , Halis Rasmina , Jasni Jasronita
Published: November 2020

This paper presents an investigation on the physio-mechanical properties and AC breakdown voltage of the Kenaf paper in the presence of Polyvinyl Alcohol (PVA) for transformers application. Kenaf bast fibers were used in order to produce the paper through the soda pulping process. The pulps were subjected to beating up to 12,000 revolutions, whereby the PVA was added to the pulps at a different weight percentage concentration up to 12%. Morphological study was carried out on the Kenaf paper based on Scanning Electron Microscopy (SEM). The apparent density, Tensile Index (TI), Burst Index (BI), Tear Index (TeI), and AC breakdown voltage of the Kenaf paper were measured. It is found that the TI and BI of Kenaf paper can be slightly improved through the introduction of PVA. On other hand, the TeI of the Kenaf paper decreases with the increment of the PVA. The AC breakdown voltage of the Kenaf paper slightly increases with the increment of PVA weight percentage concentration.
Machining of Titanium Metal Matrix Composites: Progress Overview
Authors: Escaich Cécile , Shi Zhongde , Baron Luc , Balazinski Marek
Published: November 2020

The TiC particles in titanium metal matrix composites (TiMMCs) make them difficult to machine. As a specific MMC, it is legitimate to wonder if the cutting mechanisms of TiMMCs are the same as or similar to those of MMCs. For this purpose, the tool wear mechanisms for turning, milling, and grinding are reviewed in this paper and compared with those for other MMCs. In addition, the chip formation and morphology, the material removal mechanism and surface quality are discussed for the different machining processes and examined thoroughly. Comparisons of the machining mechanisms between the TiMMCs and MMCs indicate that the findings for other MMCs should not be taken for granted for TiMMCs for the machining processes reviewed. The increase in cutting speed leads to a decrease in roughness value during grinding and an increase of the tool life during turning. Unconventional machining such as laser-assisted turning is effective to increase tool life. Under certain conditions, a “wear shield” was observed during the early stages of tool wear during turning, thereby increasing tool life considerably. The studies carried out on milling showed that the cutting parameters affecting surface roughness and tool wear are dependent on the tool material. The high temperatures and high shears that occur during machining lead to microstructural changes in the workpiece during grinding, and in the chips during turning. The adiabatic shear band (ASB) of the chips is the seat of the sub-grains’ formation. Finally, the cutting speed and lubrication influenced dust emission during turning but more studies are needed to validate this finding. For the milling or grinding, there are major areas to be considered for thoroughly understanding the machining behavior of TiMMCs (tool wear mechanisms, chip formation, dust emission, etc.).
Enhancing Electrical Contact with a Commercial Polymer for Electrical Resistivity Tomography on Archaeological Sites: A Case Study
Authors: Vásconez-Maza Marco D. , Martínez-Pagán Pedro , Aktarakçi Hasan , García-Nieto María C. , Martínez-Segura Marcos A.
Published: November 2020

This communication reports an improvement of the quality of the electrical data obtained from the application of electrical resistivity tomography method on archaeological studies. The electrical contact between ground and electrode enhances significantly by using carbomer-based gel during the electrical resistivity tomography measurements. Not only does the gel promote the conservation of the building surface under investigation, but it also virtually eliminates the necessity of conventional spike electrodes, which in many archaeological studies are inadequate or not permitted. Results evidenced an enhancement in the quality of the electrical data obtained in the order of thousands of units compared with those without using the carbomer-based gel. The potential and capabilities of this affordable gel make it appropriate to be applied to other geoelectrical studies beyond archaeological investigations. Moreover, it might solve corrosion issues on conventional spike electrodes, and electrical multicore cables usually provoked for added saltwater attempting to improve the electrical contact.
Green Synthesis of Co-Zn Spinel Ferrite Nanoparticles: Magnetic and Intrinsic Antimicrobial Properties
Authors: Omelyanchik Alexander , Levada Kateryna , Pshenichnikov Stanislav , Abdolrahim Maryam , Baricic Miran , Kapitunova Anastasiya , Galieva Alima , Sukhikh Stanislav , Astakhova Lidiia , Antipov Sergey , Fabiano Bruno , Peddis Davide , Rodionova Valeria
Published: November 2020

Spinel ferrite magnetic nanoparticles have attracted considerable attention because of their high and flexible magnetic properties and biocompatibility. In this work, a set of magnetic nanoparticles of cobalt ferrite doped with zinc was synthesized via the eco-friendly sol-gel auto-combustion method. Obtained particles displayed a room-temperature ferromagnetic behavior with tuned by chemical composition values of saturation magnetization and coercivity. The maximal values of saturation magnetization ~74 Am2/kg were found in cobalt ferrite nanoparticles with a 15–35% molar fraction of cobalt replaced by zinc ions. At the same time, the coercivity exhibited a gradually diminishing trend from ~140 to ~5 mT whereas the concentration of zinc was increased from 0 to 100%. Consequently, nanoparticles produced by the proposed method possess highly adjustable magnetic properties to satisfy the requirement of a wide range of possible applications. Further prepared nanoparticles were tested with bacterial culture to display the influence of chemical composition and magnetic structure on nanoparticles-bacterial cell interaction.
Comparison of Different Approaches for Testing Sorption by a Superabsorbent Polymer to Be Used in Cement-Based Materials
Authors: Zhong Peihua , Wang Jun , Wang Xiaoxian , Liu Jiaping , Li Zhen , Zhou Yichuan
Published: November 2020

The absorption and desorption behavior of superabsorbent polymer (SAP) can influence various properties of cementitious materials. Therefore, it is essential to know these performances of SAP prior to implementation in cement-based materials. In this paper, two types of SAP with different chemical compositions were tested in free liquid (deionized water and cement filtrate) and cement paste. Five absorption test methods were considered, including the tea-bag method, the filtration method, the centrifuge method, the suction filtration method, and the slump flow method. The results show that the absorptivity of SAP A73 and SAP N in cement paste by the slump flow method are about 21 g/g and 7 g/g, respectively. In addition, the centrifuge method and suction filtration method give more accurate absorption values when compared to the tea-bag method and filtration method due to their effectiveness in removing inter-particle liquid. Though the absorptivity obtained by the tea-bag method is higher than the centrifuge method and suction filtration method, it is still a good pre-test method to reveal the performance of SAP used in cementitious materials due to time-saving and simple setups.
Designing Limit-Cycle Suppressor Using Dithering and Dual-Input Describing Function Methods
Authors: Mbitu Elisabeth Tansiana , Chen Seng-Chi
Published: November 2020

This paper described a method to design a limit-cycle suppressor. The dithering technique was used to eliminate self-sustained oscillations or limit cycles. Otherwise, the Dual Input Describing Function (DIDF) method was applied to design dither parameters and analyze the existence of limit cycles. This method was done in a nonlinear system with relay nonlinearity using three standard dither signals, namely sine, triangle, and square waves. The aim of choosing varying dithers was to investigate the effect of dither shapes and the minimum amplitude required for the quenching strategy. First, the possibility and amplitude of limit cycles were determined graphically on the DIDF curve. Then, the minimum amplitude of dither was calculated based on the DIDF analysis. Finally, a simulation was built to verify the analytical work using a digital computer. The simulation results were related to the analysis results. It was evident that the dithering technique is a simple way to suppress limit cycles in a nonlinear system. This paper also presented that dither is an amplitude function, and square-wave dither has the minimum amplitude to quench limit cycles.
Machinery Failure Approach and Spectral Analysis to Study the Reaction Time Dynamics over Consecutive Visual Stimuli: An Entropy-Based Model
Authors: Iglesias-Martínez Miguel E. , Hernaiz-Guijarro Moisés , Castro-Palacio Juan Carlos , Fernández-de-Córdoba Pedro , Isidro J. M. , Navarro-Pardo Esperanza
Published: November 2020

The reaction times of individuals over consecutive visual stimuli have been studied using an entropy-based model and a failure machinery approach. The used tools include the fast Fourier transform and a spectral entropy analysis. The results indicate that the reaction times produced by the independently responding individuals to visual stimuli appear to be correlated. The spectral analysis and the entropy of the spectrum yield that there are features of similarity in the response times of each participant and among them. Furthermore, the analysis of the mistakes made by the participants during the reaction time experiments concluded that they follow a behavior which is consistent with the MTBF (Mean Time Between Failures) model, widely used in industry for the predictive diagnosis of electrical machines and equipment.
Immersed Boundary Method for Simulating Interfacial Problems
Authors: Lee Wanho , Lee Seunggyu
Published: November 2020

We review the immersed boundary (IB) method in order to investigate the fluid-structure interaction problems governed by the Navier–Stokes equation. The configuration is described by the Lagrangian variables, and the velocity and pressure of the fluid are defined in Cartesian coordinates. The interaction between two different coordinates is involved in a discrete Dirac-delta function. We describe the IB method and its numerical implementation. Standard numerical simulations are performed in order to show the effect of the parameters and discrete Dirac-delta functions. Simulations of flow around a cylinder and movement of Caenorhabditis elegans are introduced as rigid and flexible boundary problems, respectively. Furthermore, we provide the MATLAB codes for our simulation.