bluuebunny/crossref_metadata_2025 huggingface dataset:
Differential Factors Are Associated with Physical Activity in Older Adults in Brazil with and without Non-Communicable Chronic Diseases: A Cross-Sectional Analysis of the 2019 National Health Survey
Authors: Pereira Zainovan Serrão , da Silva Amanda Santos , Melo João Carlos do Nascimento , dos Santos Jullyane Caldas , Sewo Sampaio Priscila Yukari , Silva Roberto Jerônimo dos Santos , Araújo Raphael Henrique de Oliveira , Sampaio Ricardo Aurélio Carvalho
Published: July 2023

This study analyzed whether sociodemographic factors, health perception, dietary habits, and screen time are related to physical activity (PA) in older people with and without non-communicable chronic diseases (NCDs). This cross-sectional study analyzed data from the 2019 Brazilian National Health Survey; the sample was older adults (≥60 years old; n = 22,726). The outcome of this study was being physically active or inactive during leisure time, and NCD was used as a moderating variable. The correlates investigated were sociodemographic and health-related variables. According to the logistic regression analysis, it was observed that being male had an association only in the group with NCDs (OR = 1.25 (1.05–1.48)), as well as residing in the northeastern region (OR = 1.26 (1.04–1.53)). On the other hand, high levels of education (OR = 4.09 (2.92–5.2); OR = 1.92 (1.48–2.49)) and income (OR = 1.64 (1.09–2.48); OR = 1.86 (1.33–2.60)) were associated with PA in both groups, as well as dietary habits (OR = 1.03 (1.01–1.05); (OR = 1.05 (1.04–1.07)). Advanced age (OR = 0.96 (0.94–0.97); OR = 0.97 (0.96–0.98)) and reporting a regular health perception (OR = 0.53 (0.43–0.66); OR = 0.61 (0.52–0.73)) were factors associated with physical inactivity in both groups. Gender, education, and income were unequally associated with an active lifestyle in both groups, and therefore, barriers to PA may arise.
Topic-Clustering Model with Temporal Distribution for Public Opinion Topic Analysis of Geospatial Social Media Data
Authors: Hu Chunchun , Liang Qin , Luo Nianxue , Lu Shuixiang
Published: July 2023

Analysis of the spatiotemporal distribution of online public opinion topics can help understand the hotspots of public concern. The topic model is employed widely in public opinion topic clustering for social media data. In order to handle topic-clustering of low-quality geospatial social media data, such as microblog data, with short text and timeliness characteristics, this study proposed a Dirichlet multinomial mixture over time (DMMOT) model to cluster microblog topic for public opinion analysis. The DMMOT model assumes that a single document belongs to a single topic, in line with the characteristics of a short text, and it introduces the probability distribution of “topic-time” in the process of topic generation. The model parameter inference process was presented in detail by exploring the Gibbs sampling method. Results generated using the DMMOT model in case study show that the “topic-word” distribution is semantically aggregated within various topics, and “topic-time” distribution clustered within a time window under each topic. Furthermore, the characteristics of the trend of each topic over time are basically consistent with the corresponding trend of topic in reality in terms of content. These indicate that the DMMOT model improves topic clustering for short text to some extent. Furthermore, the DMMOT model performed well in both temporal and spatial analysis of public opinion topics based on microblog data.
Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica
Authors: López Dariel , Larama Giovanni , Sáez Patricia L. , Bravo León A.
Published: July 2023

Warming in the Antarctic Peninsula is one of the fastest on earth, and is predicted to become more asymmetric in the near future. Warming has already favored the growth and reproduction of Antarctic plant species, leading to a decrease in their freezing tolerance (deacclimation). Evidence regarding the effects of diurnal and nocturnal warming on freezing tolerance-related gene expression in D. antarctica is negligible. We hypothesized that freezing tolerance-related gene (such as CBF-regulon) expression is reduced mainly by nocturnal warming rather than diurnal temperature changes in D. antarctica. The present work aimed to determine the effects of diurnal and nocturnal warming on cold deacclimation and its associated gene expression in D. antarctica, under laboratory conditions. Fully cold-acclimated plants (8 °C/0 °C), with 16h/8h thermoperiod and photoperiod duration, were assigned to four treatments for 14 days: one control (8 °C/0 °C) and three with different warming conditions (diurnal (14 °C/0 °C), nocturnal (8 °C/6 °C), and diurnal-nocturnal (14 °C/6 °C). RNA-seq was performed and differential gene expression was analyzed. Nocturnal warming significantly down-regulated the CBF transcription factors expression and associated cold stress response genes and up-regulated photosynthetic and growth promotion genes. Consequently, nocturnal warming has a greater effect than diurnal warming on the cold deacclimation process in D. antarctica. The eco-physiological implications are discussed.
Preparation of Xanthene-TEMPO Dyads: Synthesis and Study of the Radical Enhanced Intersystem Crossing
Authors: Zhu Wenhui , Wu Yanran , Zhang Yiyan , Sukhanov Andrey A. , Chu Yuqi , Zhang Xue , Zhao Jianzhang , Voronkova Violeta K.
Published: July 2023

We prepared a rhodamine-TEMPO chromophore-radical dyad (RB-TEMPO) to study the radical enhanced intersystem crossing (REISC). The visible light-harvesting chromophore rhodamine is connected with the TEMPO (a nitroxide radical) via a C–N bond. The UV-vis absorption spectrum indicates negligible electron interaction between the two units at the ground state. Interestingly, the fluorescence of the rhodamine moiety is strongly quenched in RB-TEMPO, and the fluorescence lifetime of the rhodamine moiety is shortened to 0.29 ns, from the lifetime of 3.17 ns. We attribute this quenching effect to the intramolecular electron spin–spin interaction between the nitroxide radical and the photoexcited rhodamine chromophore. Nanosecond transient absorption spectra confirm the REISC in RB-TEMPO, indicated by the detection of the rhodamine chromophore triplet excited state; the lifetime was determined as 128 ns, which is shorter than the native rhodamine triplet state lifetime (0.58 μs). The zero-field splitting (ZFS) parameters of the triplet state of the chromophore were determined with the pulsed laser excited time-resolved electron paramagnetic resonance (TREPR) spectra. RB-TEMPO was used as a photoinitiator for the photopolymerization of pentaerythritol triacrylate (PETA). These studies are useful for the design of heavy atom-free triplet photosensitizers, the study of the ISC, and the electron spin dynamics of the radical-chromophore systems upon photoexcitation.
Numerous Trigger-like Interactions of Kinases/Protein Phosphatases in Human Skeletal Muscles Can Underlie Transient Processes in Activation of Signaling Pathways during Exercise
Authors: Vertyshev Alexander Yu. , Akberdin Ilya R. , Kolpakov Fedor A.
Published: July 2023

Optimizing physical training regimens to increase muscle aerobic capacity requires an understanding of the internal processes that occur during exercise that initiate subsequent adaptation. During exercise, muscle cells undergo a series of metabolic events that trigger downstream signaling pathways and induce the expression of many genes in working muscle fibers. There are a number of studies that show the dependence of changes in the activity of AMP-activated protein kinase (AMPK), one of the mediators of cellular signaling pathways, on the duration and intensity of single exercises. The activity of various AMPK isoforms can change in different directions, increasing for some isoforms and decreasing for others, depending on the intensity and duration of the load. This review summarizes research data on changes in the activity of AMPK, Ca2+/calmodulin-dependent protein kinase II (CaMKII), and other components of the signaling pathways in skeletal muscles during exercise. Based on these data, we hypothesize that the observed changes in AMPK activity may be largely related to metabolic and signaling transients rather than exercise intensity per se. Probably, the main events associated with these transients occur at the beginning of the exercise in a time window of about 1–10 min. We hypothesize that these transients may be partly due to putative trigger-like kinase/protein phosphatase interactions regulated by feedback loops. In addition, numerous dynamically changing factors, such as [Ca2+], metabolite concentration, and reactive oxygen and nitrogen species (RONS), can shift the switching thresholds and change the states of these triggers, thereby affecting the activity of kinases (in particular, AMPK and CaMKII) and phosphatases. The review considers the putative molecular mechanisms underlying trigger-like interactions. The proposed hypothesis allows for a reinterpretation of the experimental data available in the literature as well as the generation of ideas to optimize future training regimens.
Eukaryotic Cell Membranes: Structure, Composition, Research Methods and Computational Modelling
Authors: Zhukov Anatoly , Popov Valery
Published: July 2023

This paper deals with the problems encountered in the study of eukaryotic cell membranes. A discussion on the structure and composition of membranes, lateral heterogeneity of membranes, lipid raft formation, and involvement of actin and cytoskeleton networks in the maintenance of membrane structure is included. Modern methods for the study of membranes and their constituent domains are discussed. Various simplified models of biomembranes and lipid rafts are presented. Computer modelling is considered as one of the most important methods. This is stated that from the study of the plasma membrane structure, it is desirable to proceed to the diverse membranes of all organelles of the cell. The qualitative composition and molar content of individual classes of polar lipids, free sterols and proteins in each of these membranes must be considered. A program to create an open access electronic database including results obtained from the membrane modelling of individual cell organelles and the key sites of the membranes, as well as models of individual molecules composing the membranes, has been proposed.
EPs® 7630 Stimulates Tissue Repair Mechanisms and Modifies Tight Junction Protein Expression in Human Airway Epithelial Cells
Authors: Fang Lei , Zhou Liang , Kulić Žarko , Lehner Martin D. , Tamm Michael , Roth Michael
Published: July 2023

Airway epithelium repair after infection consists of wound repair, re-synthesis of the extracellular matrix (ECM), and tight junction proteins. In humans, EPs® 7630 obtained from Pelargonium sidoides roots reduces the severity and duration of acute respiratory tract infections. The effect of EPs® 7630 on tissue repair of rhinovirus-16 (RV-16) infected and control human airway epithelial cells was assessed for: (i) epithelial cell proliferation by manual cell counts, (ii) epithelial wound repair by “scratch assay”, (iii) ECM composition by Western-blotting and cell-based ELISA, and (iv) epithelial tight junction proteins by Western-blotting. EPs® 7630 stimulated cell proliferation through cAMP, CREB, and p38 MAPK. EPs® 7630 significantly improved wound repair. Pro-inflammatory collagen type-I expression was reduced by EPs® 7630, while fibronectin was increased. Virus-binding tight junction proteins desmoglein2, desmocollin2, ZO-1, claudin1, and claudin4 were downregulated by EPs® 7630. The RV16-induced shift of the ECM towards the pro-inflammatory type was prevented by EPs® 7630. Most of the effects of EPs® 7630 on tissue repair and regeneration were sensitive to inhibition of cAMP-induced signaling. The data suggest that EPs® 7630-dependent modification of epithelial cell metabolism and function might underlie the faster recovery time from viral infections, as reported by others in clinical studies.
Innate Immunity in Cancer Biology and Therapy
Authors: Zhang Yuxia , Xue Wenjing , Xu Caili , Nan Yanyang , Mei Shuang , Ju Dianwen , Wang Shaofei , Zhang Xuyao
Published: July 2023

Immunotherapies including adaptive immune checkpoint inhibitors (ICIs) and chimeric antigen receptor (CAR) T cells, have developed the treatment of cancer in clinic, and most of them focus on activating T cell immunity. Although these strategies have obtained unprecedented clinical responses, only limited subsets of cancer patients could receive long-term benefits, highlighting the demand for identifying novel targets for the new era of tumor immunotherapy. Innate immunity has been demonstrated to play a determinative role in the tumor microenvironment (TME) and influence the clinical outcomes of tumor patients. A thorough comprehension of the innate immune cells that infiltrate tumors would allow for the development of new therapeutics. In this review, we outline the role and mechanism of innate immunity in TME. Moreover, we discuss innate immunity-based cancer immunotherapy in basic and clinical studies. Finally, we summarize the challenges in sufficiently motivating innate immune responses and the corresponding strategies and measures to improve anti-tumor efficacy. This review could aid the comprehension of innate immunity and inspire the creation of brand-new immunotherapies for the treatment of cancer.
Spinal Cord Blood Vessels in Aged Mice Show Greater Levels of Hypoxia-Induced Vascular Disruption and Microglial Activation
Authors: Halder Sebok K. , Milner Richard
Published: July 2023

In response to chronic mild hypoxia (CMH, 8% O2), spinal cord blood vessels launch a robust angiogenic response that is associated with transient disruption of the blood–spinal cord barrier (BSCB) which, in turn, triggers a microglial vasculo-protective response. Because hypoxia occurs in many age-related conditions, the goal of this study was to define how aging influences these responses by comparing events in young (8–10 weeks) and aged (20 months) mice. This revealed that aged mice had much greater (3–4-fold) levels of hypoxic-induced BSCB disruption than young mice and that, while the early stage of the angiogenic response in aged mice was no different to young mice, the maturation of newly formed vessels was significantly delayed. Interestingly, microglia in the spinal cords of aged mice were much more activated than young mice, even under normoxic conditions, and this was further enhanced by CMH, though, surprisingly, this resulted in reduced microglial clustering around leaky blood vessels and diminished vasculo-protection. Vascular disruption was associated with loss of myelin in spinal cord white matter (WM) in both young and aged mice. Furthermore, it was notable that the spinal cord of aged mice contained a lower density of Olig2+ oligodendroglial cells even under normoxic conditions and that CMH significantly reduced the density of Olig2+ cells in spinal cord WM of the aged, but not the young, mice. These results demonstrate that spinal cord blood vessels of aged mice are much more vulnerable to the damaging effects of hypoxia than young mice, in part due to the reduced vasculo-protection conferred by chronically activated microglial cells. These observations may have implications for the pathogenesis and/or treatment of spinal cord diseases such as amyotrophic lateral sclerosis (ALS) and suggest that an improvement in microglial function could offer therapeutic potential for treating these age-related conditions.
Molecular Mechanism of Zinc-Dependent Oligomerization of Alzheimer’s Amyloid-β with Taiwan (D7H) Mutation
Authors: Kechko Olga I. , Adzhubei Alexei A. , Tolstova Anna P. , Indeykina Maria I. , Popov Igor A. , Zhokhov Sergey S. , Gnuchev Nikolay V. , Mitkevich Vladimir A. , Makarov Alexander A. , Kozin Sergey A.
Published: July 2023

Amyloid-β (Aβ) is a peptide formed by 39–43 amino acids, heterogenous by the length of its C-terminus. Aβ constitutes a subnanomolar monomeric component of human biological fluids; however, in sporadic variants of Alzheimer’s disease (AD), it forms soluble neurotoxic oligomers and accumulates as insoluble extracellular polymeric aggregates (amyloid plaques) in the brain tissues. The plaque formation is controlled by zinc ions; therefore, abnormal interactions between the ions and Aβ seem to take part in the triggering of sporadic AD. The amyloid plaques contain various Aβ isoforms, among which the most common is Aβ with an isoaspartate in position 7 (isoD7). The spontaneous conversion of D7 to isoD7 is associated with Aβ aging. Aβ molecules with isoD7 (isoD7-Aβ) easily undergo zinc-dependent oligomerization, and upon administration to transgenic animals (mice, nematodes) used for AD modeling, act as zinc-dependent seeds of the pathological aggregation of Aβ. The formation of zinc-bound homo- and hetero-oligomers with the participation of isoD7-Aβ is based on the rigidly structured segment 11-EVHH-14, located in the Aβ metal binding domain (Aβ16). Some hereditary variants of AD are associated with familial mutations within the domain. Among these, the most susceptible to zinc-dependent oligomerization is Aβ with Taiwan (D7H) mutation (D7H-Aβ). In this study, the D7H-Aβ metal binding domain (D7H-Aβ16) has been used as a model to establish the molecular mechanism of zinc-induced D7H-Aβ oligomerization through turbidimetry, dynamic light scattering, isothermal titration calorimetry, mass spectrometry, and computer modelling. Additionally, the modeling data showed that a molecule of D7H-Aβ, as well as isoD7-Aβ in combination with two Aβ molecules, renders a stable zinc-induced heterotrimer. The trimers are held together by intermolecular interfaces via zinc ions, with the primary interfaces formed by 11-EVHH-14 sites of the interacting trimer subunits. In summary, the obtained results confirm the role of the 11-EVHH-14 region as a structure and function determinant for the zinc-dependent oligomerization of all known Aβ species (including various chemically modified isoforms and AD-associated mutants) and point at this region as a potent target for drugs aimed to stop amyloid plaque formation in both sporadic and hereditary variants of AD.
Differential Contributions of mSWI/SNF Chromatin Remodeler Sub-Families to Myoblast Differentiation
Authors: Padilla-Benavides Teresita , Olea-Flores Monserrat , Sharma Tapan , Syed Sabriya A. , Witwicka Hanna , Zuñiga-Eulogio Miriam D. , Zhang Kexin , Navarro-Tito Napoleon , Imbalzano Anthony N.
Published: July 2023

Mammalian SWI/SNF (mSWI/SNF) complexes are ATP-dependent chromatin remodeling enzymes that are critical for normal cellular functions. mSWI/SNF enzymes are classified into three sub-families based on the presence of specific subunit proteins. The sub-families are Brm- or Brg1-associated factor (BAF), ncBAF (non-canonical BAF), and polybromo-associated BAF (PBAF). The biological roles for the different enzyme sub-families are poorly described. We knocked down the expression of genes encoding unique subunit proteins for each sub-family, Baf250A, Brd9, and Baf180, which mark the BAF, ncBAF, and PBAF sub-families, respectively, and examined the requirement for each in myoblast differentiation. We found that Baf250A and the BAF complex were required to drive lineage-specific gene expression. KD of Brd9 delayed differentiation. However, while the Baf250A-dependent gene expression profile included myogenic genes, the Brd9-dependent gene expression profile did not, suggesting Brd9 and the ncBAF complex indirectly contributed to differentiation. Baf180 was dispensable for myoblast differentiation. The results distinguish between the roles of the mSWI/SNF enzyme sub-families during myoblast differentiation.
Use of Enzymatically Activated Carbon Monoxide Donors for Sensitizing Drug-Resistant Tumor Cells
Authors: Sodano Federica , Rolando Barbara , Lazzarato Loretta , Costamagna Costanzo , Failla Mariacristina , Riganti Chiara , Chegaev Konstantin
Published: July 2023

The application of gaseous signaling molecules like NO, H2S or CO to overcome the multidrug resistance in cancer treatment has proven to be a viable therapeutic strategy. The development of CO-releasing molecules (CORMs) in a controlled manner and in targeted tissues remains a challenge in medicinal chemistry. In this paper, we describe the design, synthesis and chemical and enzymatic stability of a novel non-metal CORM (1) able to release intracellularly CO and, simultaneously, facilitate fluorescent degradation of products under the action of esterase. The toxicity of 1 against different human cancer cell lines and their drug-resistant counterparts, as well as the putative mechanism of toxicity were investigated. The drug-resistant cancer cell lines efficiently absorbed 1 and 1 was able to restore their sensitivity vs. chemotherapeutic drugs by causing a CO-dependent mitochondrial oxidative stress that culminated in mitochondrial-dependent apoptosis. These results demonstrate the importance of CORMs in cases where conventional chemotherapy fails and thus open the horizons towards new combinatorial strategies to overcome multidrug resistance.
Optimization of Protocols for the Induction of Callus and Plant Regeneration in White Clover (Trifolium repens L.)
Authors: Qi Tiangang , Tang Tao , Zhou Qinyu , Yang Weiqiang , Hassan Muhammad Jawad , Cheng Bizhen , Nie Gang , Li Zhou , Peng Yan
Published: July 2023

White clover is a widely grown temperate legume forage with high nutritional value. Research on the functional genomics of white clover requires a stable and efficient transformation system. In this study, we successfully induced calluses from the cotyledons and leaves of 10 different white clover varieties. The results showed that the callus formation rate in the cotyledons did not vary significantly among the varieties, but the highest callus formation rate was observed in ‘Koala’ leaves. Subsequently, different concentrations of antioxidants and hormones were tested on the browning rate and differentiation ability of the calluses, respectively. The results showed that the browning rate was the lowest on MS supplemented with 20 mg L−1 AgNO3 and 25 mg L−1 VC, respectively, and the differentiation rate was highest on MS supplemented with 1 mg L−1 6-BA, 1 mg L−1 KT and 0.5 mg L−1 NAA. In addition, the transformation system for Agrobacterium tumefaciens-mediated transformation of 4-day-old leaves was optimized to some extent and obtained a positive callus rate of 8.9% using green fluorescent protein (GFP) as a marker gene. According to our data, by following this optimized protocol, the transformation efficiency could reach 2.38%. The results of this study will provide the foundation for regenerating multiple transgenic white clover from a single genetic background.
A Novel Authentication Method That Combines Honeytokens and Google Authenticator
Authors: Papaspirou Vassilis , Papathanasaki Maria , Maglaras Leandros , Kantzavelou Ioanna , Douligeris Christos , Ferrag Mohamed Amine , Janicke Helge
Published: July 2023

Despite the rapid development of technology, computer systems still rely heavily on passwords for security, which can be problematic. Although multi-factor authentication has been introduced, it is not completely effective against more advanced attacks. To address this, this study proposes a new two-factor authentication method that uses honeytokens. Honeytokens and Google Authenticator are combined to create a stronger authentication process. The proposed approach aims to provide additional layers of security and protection to computer systems, increasing their overall security beyond what is currently provided by single-password or standard two-factor authentication methods. The key difference is that the proposed system resembles a two-factor authentication but, in reality, works like a multi-factor authentication system. Multi-factor authentication (MFA) is a security technique that verifies a user’s identity by requiring multiple credentials from distinct categories. These typically include knowledge factors (something the user knows, such as a password or PIN), possession factors (something the user has, such as a mobile phone or security token), and inherence factors (something the user is, such as a biometric characteristic like a fingerprint). This multi-tiered approach significantly enhances protection against potential attacks. We examined and evaluated our system’s robustness against various types of attacks. From the user’s side, the system is as friendly as a two-factor authentication method with an authenticator and is more secure.
A Survey of Internet of Things and Cyber-Physical Systems: Standards, Algorithms, Applications, Security, Challenges, and Future Directions
Authors: Chui Kwok Tai , Gupta Brij B. , Liu Jiaqi , Arya Varsha , Nedjah Nadia , Almomani Ammar , Chaurasia Priyanka
Published: July 2023

The smart city vision has driven the rapid development and advancement of interconnected technologies using the Internet of Things (IoT) and cyber-physical systems (CPS). In this paper, various aspects of IoT and CPS in recent years (from 2013 to May 2023) are surveyed. It first begins with industry standards which ensure cost-effective solutions and interoperability. With ever-growing big data, tremendous undiscovered knowledge can be mined to be transformed into useful applications. Machine learning algorithms are taking the lead to achieve various target applications with formulations such as classification, clustering, regression, prediction, and anomaly detection. Notably, attention has shifted from traditional machine learning algorithms to advanced algorithms, including deep learning, transfer learning, and data generation algorithms, to provide more accurate models. In recent years, there has been an increasing need for advanced security techniques and defense strategies to detect and prevent the IoT and CPS from being attacked. Research challenges and future directions are summarized. We hope that more researchers can conduct more studies on the IoT and on CPS.
Deep-Learning-Based Human Chromosome Classification: Data Augmentation and Ensemble
Authors: D’Angelo Mattia , Nanni Loris
Published: July 2023

Object classification is a crucial task in deep learning, which involves the identification and categorization of objects in images or videos. Although humans can easily recognize common objects, such as cars, animals, or plants, performing this task on a large scale can be time-consuming and error-prone. Therefore, automating this process using neural networks can save time and effort while achieving higher accuracy. Our study focuses on the classification step of human chromosome karyotyping, an important medical procedure that helps diagnose genetic disorders. Traditionally, this task is performed manually by expert cytologists, which is a time-consuming process that requires specialized medical skills. Therefore, automating it through deep learning can be immensely useful. To accomplish this, we implemented and adapted existing preprocessing and data augmentation techniques to prepare the chromosome images for classification. We used ResNet-50 convolutional neural network and Swin Transformer, coupled with an ensemble approach to classify the chromosomes, obtaining state-of-the-art performance in the tested dataset.
The Effect of X-ray Irradiation on the Fitness and Field Adaptability of the Codling Moth: An Orchard Study in Northeast China
Authors: Zhang Jinghan , Huang Shengwang , Zhao Shici , Wang Xingya , Yang Xianming , Zhao Huiyuan , Gao Ping , Li Yuting , Yang Xueqing
Published: July 2023

The codling moth, Cydia pomonella (L.), is an invasive agricultural pest of pome fruits and walnuts in China that threatens the apple industry in the Loess Plateau and Bohai Bay; it has developed resistance to many insecticides. Sterile insect technique (SIT) combined with area-wide integrated pest management (AW-IPM) can reduce the risk of resistance to insecticides and effectively control some insect pest species. Our previous laboratory experiment found that irradiation with 366 Gy of X-ray caused the males of the codling moth to become sterile. However, the sterility and adaptability of males after being irradiated with 366 Gy X-ray in the field are still unclear. In this study, we investigated the effect of X-ray irradiation on the fitness of male adults that emerged from pupae irradiated with 366 Gy to explore their adaptability and mating competitiveness, and to examine the effect of releasing sterile male insects in orchards in northeast China on the fruit infestation rate of the Nanguo pear. The results showed that 366 Gy of X-ray irradiation significantly reduced the mating competitiveness of males and the hatching rate of the eggs laid by females pairing with sterile males. Meanwhile, the lifespan of the sterile male moths was significantly shorter than that of the normal ones in the field. A pilot test showed that the release twice of sterile male moths in the orchards had no significant effect on the fruit infestation rate. Our field experiments provide a scientific basis for the further optimization of the SIT technology program for controlling C. pomonella.
Taxonomic Revision of Tipula (Vestiplex Bezzi) Crane Flies (Diptera, Tipulidae) in Taiwan with Descriptions of Six New Species
Authors: Starkevich Pavel , Young Chen W.
Published: July 2023

The crane fly subgenus Tipula (Vestiplex) Bezzi, 1924 (Diptera, Tipulidae) of Taiwan is taxonomically reviewed, entailing the recognition of 18 species, with six of these species newly described, including T. (V.) diamondi sp. nov., T. (V.) formosae sp. nov., T. (V.) graciliana sp. nov., T. (V.) pseudobiserra sp. nov.,  T. (V.) survilai sp. nov., and T. (V.) taiwanica sp. nov. Three species, T. biaciculifera Alexander, 1937, T. niitakensis Alexander, 1938, and T. pseudobiaciculifera Men, Xue and Wang, 2016, listed previously as members of the subgenus T. (Pterelachisus) Rondani, 1842, are now formally placed as member taxa within T. (Vestiplex). Tipula subnata Alexander, 1949 and T. (V.) longarmata Yang and Yang, 1999 are designated as junior synonyms of T. (V.) coxitalis Alexander, 1935; T. (V.) nokonis Alexander, 1935 is designated as a junior synonym of T. (V.) terebrata Edwards, 1921; and T. (V.) takahashiana Alexander, 1938 is designated as a junior synonym of T. (V.) bicornigera Alexander, 1938. Included in this taxonomic revision are a key to species, species diagnoses for all species, complete descriptions for newly described species, and illustrations of the male genitalia for all species, and for female terminalia when available. DNA barcode sequences for all Taiwanese species of T. (Vestiplex) are provided. Males are associated with conspecific females based on CO1 results and maximum likelihood trees resulting from the analyses are presented. The Taiwan fauna of Tipula (Vestiplex) is highly endemic with 16 of the 18 species restricted to the island. At the species group level, no groups are endemic to Taiwan and the groups show closest biogeographic relationships to the Qinghai–Tibetan Plateau.
Comparison of Protein and Carbohydrate Consumption and Processing in Emerging Workers, Gynes and Males of the Wasp Polistes metricus
Authors: Gay Daniel R. , Judd Timothy M.
Published: July 2023

There is growing evidence that paper wasps’ (Polistes’) fate as workers or reproductive females (gynes) is affected by cues that exist at the larval stage and during eclosion. The nutritional requirements for workers and gynes are different early in their adult lives. Males are short-lived and have different nutritional needs than females. To determine the relative importance of larval and adult cues, we reared Polistes metricus individuals from prepupae to adults isolated from known environmental cues shown to affect caste differentiation. Individuals were given access to two foods with different ratios of protein and carbohydrates. Levels of protein, amino acids, carbohydrates and lipids were measured after the feeding trials. If larval experience drove feeding behavior in adults, we expected to see differences in protein and carbohydrate intake as well as differences in nutrient levels. Females showed no differences in feeding or nutrient levels. Males had lower levels of protein and amino acids than females but had similar feeding results to females.
Qualitative Analyses of Textile Damage (Cuts and Tears) Applied to Fabrics Exposed to the Decomposition of Carcasses and Associated Insect Activity in an Austral Summer
Authors: Ziogos Sotirios , Dadour Ian R. , Pitts Kari , Magni Paola A.
Published: July 2023

Fatal stabbings are the leading cause of homicide in countries with restricted access to firearms, such as Australia. The analysis of damage on clothing imparted by a sharp object can assist in the characterization of the weapon. However, decomposition and carrion insects can modify the features of the damage, impeding textile damage analysis and crime reconstruction. This study aimed to identify and characterize the modifications of textile damage over 47 days of decomposition during the summer season in Western Australia. Fabric modifications were analyzed on cotton, synthetic, and blended fabrics with standardized cuts and tears, wrapped on 99 stillborn piglets. Six unclothed piglets acted as controls, with three being stabbed. All piglets were placed simultaneously in the field alongside swatches of fabric. Analyses considered taphonomy, insect interactions, and any textile damage using optical microscopy and SEM. The results showed that carrion insects can modify existing cuts and tears and introduce new artifacts on textiles. The 100% cotton fabric was the most affected by mechanical and chemical degradation, especially cuts and areas stained with blood or decomposition fluids. The study highlights the combined effect of multiple factors on textile damage, including the type of fabric, initial damage, bloating, insect activity, and biodegradation.
IoT-Enabled Smart Drip Irrigation System Using ESP32
Authors: Pereira Gilroy P. , Chaari Mohamed Z. , Daroge Fawwad
Published: July 2023

Agriculture, or farming, is the science of cultivating the soil, growing crops, and raising livestock. Ever since the days of the first plow from sticks over ten thousand years ago, agriculture has always depended on technology. As technology and science improved, so did the scale at which farming was possible. With the popularity and growth of the Internet of Things (IoT) in recent years, there are even more avenues for technology to make agriculture more efficient and help farmers in every nation. In this paper, we designed a smart IoT-enabled drip irrigation system using ESP32 to automate the irrigation process, and we tested it. The ESP32 communicates with the Blynk app, which is used to collect irrigation data, manually water the plants, switch off the automatic watering function, and plot graphs based on the readings of the sensors. We connected the ESP32 to a soil moisture sensor, temperature sensor, air humidity sensor, and water flow sensor. The ESP32 regularly checks if the soil is dry. If the soil is dry and the soil temperature is appropriate for watering, the ESP32 opens a solenoid valve and waters the plants. The amount of time to run the drip irrigation system is determined based on the flow rate measured by the water flow sensor. The ESP32 reads the humidity sensor values and notifies the user when the humidity is too high or too low. The user can switch off the automatic watering system according to the humidity value. In both primary and field tests, we found that the system ran well and was able to grow green onions.
Coronary Computed Tomography Angiography and Abdominal Aortic Calcification Screening among High-Risk Living Kidney Donors
Authors: Skalsky Keren , Shiyovich Arthur , Hochwald Nir , Levi Amos , Zreik Lutof , Tamir Shlomit , Shafir Gideon , Briger Anat , Rahamimov Ruth , Kornowski Ran , Hamdan Ashraf
Published: July 2023

Background: A unique scanning protocol combining coronary computed tomography angiography (CTA) with routine abdominal CTA is being used at the Rabin Medical Center as a method of screening high-risk candidates for living kidney donation. We aim to evaluate the potential impact of coronary CTA on the decision regarding eligibility for kidney donation and its correlation with abdominal aortic calcification (AAC). Methods: CCTA and abdominal CTA results of potential living kidney donors evaluated for donation between September 2020 and November 2021 were retrieved. A retrospective analysis of the abdominal CTA was used to calculate the AAC. Patients’ demographic, clinical, and imaging data were collected from the electronic files, as well as the final decision regarding eligibility for donation. Results: A total of 62 potential kidney donors were evaluated for donation using the combined scan. The mean age was 53.8 years, with male predominance (59.7%). Significant coronary artery stenosis (≥70% luminal stenosis) was present in two patients (3.2%), whereas five patients (8%) had moderate stenosis (50–69%). Thirteen patients (21%) were disqualified from donation due to moderate-to-significant coronary artery disease or abdominal atherosclerosis. The correlation between the coronary artery calcium score and the AAC was found to be positive, with a Pearson correlation coefficient of 0.88 (p < 0.001). Conclusions: The use of coronary CTA in the evaluation of potential kidney donors may has a potential impact on the decision regarding eligibility for donation. A high correlation between the coronary artery calcium score and the AAC was found.
The 10 “Cardinal Sins” in the Clinical Diagnosis and Treatment of Endometriosis: A Bayesian Approach
Authors: Koninckx Philippe R. , Ussia Anastasia , Gordts Stephan , Keckstein Jörg , Saridogan Ertan , Malzoni Mario , Stepanian Assia , Setubal Antonio , Adamyan Leila , Wattiez Arnaud
Published: July 2023

Evidence-based data for endometriosis management are limited. Experiments are excluded without adequate animal models. Data are limited to symptomatic women and occasional observations. Hormonal medical therapy cannot be blinded if recognised by the patient. Randomised controlled trials are not realistic for surgery, since endometriosis is a variable disease with low numbers. Each diagnosis and treatment is an experiment with an outcome, and experience is the means by which Bayesian updating, according to the past, takes place. If the experiences of many are similar, this holds more value than an opinion. The combined experience of a group of endometriosis surgeons was used to discuss problems in managing endometriosis. Considering endometriosis as several genetically/epigenetically different diseases is important for medical therapy. Imaging cannot exclude endometriosis, and diagnostic accuracy is limited for superficial lesions, deep lesions, and cystic corpora lutea. Surgery should not be avoided for emotional reasons. Shifting infertility treatment to IVF without considering fertility surgery is questionable. The concept of complete excision should be reconsidered. Surgeons should introduce quality control, and teaching should move to explain why this occurs. The perception of information has a personal bias. These are the major problems involved in managing endometriosis, as identified by the combined experience of the authors, who are endometriosis surgeons.
MicroRNA-27b Impairs Nrf2-Mediated Angiogenesis in the Progression of Diabetic Foot Ulcer
Authors: Sakshi Shukla , Jayasuriya Ravichandran , Sathish Kumar Rajappan Chandra , Umapathy Dhamodharan , Gopinathan Athira , Balamurugan Ramachandran , Ganesan Kumar , Ramkumar Kunka Mohanram
Published: July 2023

Nuclear factor erythroid-2-related factor 2 (Nrf2) is a stress-activated transcription factor regulating antioxidant genes, and a deficiency thereof, slowing lymphangiogenesis, has been reported in diabetic foot ulcer (DFU). The mode of Nrf2 regulation in DFU has been less explored. Emerging studies on miRNA-mediated target regulation show miRNA to be the leading player in the pathogenesis of the disease. In the present study, we demonstrated the role of miR-27b in regulating Nrf2-mediated angiogenesis in DFU. A lower expression of mRNA targets, such as Nrf2, HO-1, SDF-1α, and VEGF, was observed in tissue biopsied from chronic DFU subjects, which was in line with miR-27b, signifying a positive correlation with Nrf2. Similarly, we found significantly reduced expression of miR-27b and target mRNAs Nrf2, HO-1, SDF-1α, and VEGF in endothelial cells under a hyperglycemic microenvironment (HGM). To confirm the association of miR-27b on regulating Nrf2-mediated angiogenesis, we inhibited its expression through RNA interference-mediated knockdown and observed disturbances in angiogenic signaling with reduced endothelial cell migration. In addition, to explore the role of miR-27b and angiogenesis in the activation of Nrf2, we pretreated the endothelial cells with two well-known pharmacological compounds—pterostilbene and resveratrol. We observed that activation of Nrf2 through these compounds ameliorates impaired angiogenesis on HGM-induced endothelial cells. This study suggests a positive role of miR-27b in regulating Nrf2, which seems to be decreased in DFU and improves on treatment with pterostilbene and resveratrol.
Multiple Sclerosis: Diagnosis, Management, and Future Opportunities
Authors: Maida Elisabetta , Lavorgna Luigi
Published: July 2023

Multiple sclerosis (MS) is one of the most common inflammatory neurological diseases which leads to a highly heterogeneous set of symptoms and signs due to the differential involvement of the motor, sensory, visual, and autonomic systems [...]
Impact of COVID-19 Diagnosis on Mortality in Patients with Ischemic Stroke Admitted during the 2020 Pandemic in Italy
Authors: De Luca Leonardo , D’Errigo Paola , Rosato Stefano , Badoni Gabriella , Giordani Barbara , Mureddu Gian Francesco , Tavilla Andrea , Seccareccia Fulvia , Baglio Giovanni
Published: July 2023

Aims. The impact of the COVID-19 pandemic on the event rate of patients with ischemic stroke has been poorly investigated. We sought to evaluate the impact of the COVID-19 infection on mortality in patients with ischemic stroke admitted during the 2020 pandemic in Italy. Methods. We analyzed a nationwide, comprehensive, and universal administrative database of patients who were admitted for ischemic stroke during and after the national lockdown for the COVID-19 infection in 2020, and the equivalent periods over the previous 5 years in Italy. The 2020 observed hospitalization and mortality rates of stroke patients with and without COVID-19 infection were compared with the expected rates, in accordance with the trend of the previous 5 years. Results. During the period of observation, 300,890 hospitalizations for ischemic stroke occurred in Italy. In 2020, 41,302 stroke patients (1102 with concomitant COVID-19 infection) were admitted at 771 centers. The rate of admissions for ischemic stroke during the 2020 pandemic was markedly reduced compared with previous years (percentage change vs. 2015: −23.5). Based on the 5 year trend, the 2020 expected 30 day and 1 year mortality rates were 9.8% and 23.9%, respectively, and the observed incidence of death rates were 12.2% and 26.7%, respectively (both p < 0.001). After multiple corrections, higher rates of mortality were observed among patients admitted for stroke with a concomitant COVID-19 diagnosis. Conclusions. During the COVID-19 pandemic in 2020 in Italy, the rate of hospitalizations for ischemic stroke was dramatically reduced, although both the 30 day and 1 year mortality rates increased compared with the previous 5 year trend.
Predicting Penumbra Salvage and Infarct Growth in Acute Ischemic Stroke: A Multifactor Survival Game
Authors: Sirimarco Gaia , Strambo Davide , Nannoni Stefania , Labreuche Julien , Cereda Carlo , Dunet Vincent , Puccinelli Francesco , Saliou Guillaume , Meuli Reto , Eskandari Ashraf , Wintermark Max , Michel Patrik
Published: July 2023

Background. Effective treatment of acute ischemic stroke requires reperfusion of salvageable tissue. We investigated the predictors of penumbra salvage (PS) and infarct growth (IG) in a large cohort of stroke patients. Methods. In the ASTRAL registry from 2003 to 2016, we selected middle cerebral artery strokes <24 h with a high-quality CT angiography and CT perfusion. PS and IG were correlated in multivariate analyses with clinical, biochemical and radiological variables, and with clinical outcomes. Results. Among 4090 patients, 551 were included in the study, 50.8% male, mean age (±SD) 66.3 ± 14.7 years, mean admission NIHSS (±SD 13.3 ± 7.1) and median onset-to-imaging-time (IQR) 170 (102 to 385) minutes. Increased PS was associated with the following: higher BMI and lower WBC; neglect; larger penumbra; absence of early ischemic changes, leukoaraiosis and other territory involvement; and higher clot burden score. Reduced IG was associated with the following: non-smokers; lower glycemia; larger infarct core; absence of early ischemic changes, chronic vascular brain lesions, other territory involvement, extracranial arterial pathology and hyperdense middle cerebral artery sign; and higher clot burden score. When adding subacute variables, recanalization was associated with increased PS and reduced IG, and the absence of haemorrhage with reduced IG. Collateral status was not significantly associated with IG nor with PS. Increased PS and reduced IG correlated with better 3- and 12-month outcomes. Conclusion. In our comprehensive analysis, multiple factors were found to be responsible for PS or IG, the strongest being radiological features. These findings may help to better select patients, particularly for more aggressive or late acute stroke treatment.
The Impact of Intraoperative Haemodynamic Monitoring, Prediction of Hypotension and Goal-Directed Therapy on the Outcomes of Patients Treated with Posterior Fusion Due to Adolescent Idiopathic Scoliosis
Authors: Andrzejewska Agata , Miegoń Jakub , Zacha Sławomir , Skonieczna-Żydecka Karolina , Jarosz Konrad , Zacha Wojciech , Biernawska Jowita
Published: July 2023

A prospective, single-centre, non-randomised, case–control study aimed to evaluate the effectiveness of intraoperative haemodynamic monitoring, prediction of hypotension and goal-directed therapy on the outcomes of patients undergoing posterior fusion for adolescent idiopathic scoliosis (AIS). The control group (n = 35, mean age: 15 years) received standard blood pressure control during surgery, while the intervention group (n = 24, mean age: 14 years) underwent minimally invasive haemodynamic monitoring and goal-directed therapy. The intervention group showed significantly shorter durations of hypotension (mean arterial pressure < 60 mmHg), reduced hospital stays and smaller decreases in post-surgery haemoglobin levels. Additionally, the intervention group experienced shorter times from the end of surgery to extubation. These findings suggest that incorporating targeted interventions during intraoperative care for AIS patients undergoing posterior fusion can lead to improved outcomes.