bluuebunny/crossref_metadata_2025 huggingface dataset:
SİYASAL REKLAMLARIN ALIMLANMASI: STUART HALL’ÜN PERSPEKTİFİNDEN 31 MART 2019 YEREL SEÇİMLERİ ÖRNEĞİNDE BİR ANALİZ
Authors: ÇAKMAK Fatma
Published: September 2019

Siyaset ve medya, kitlelerin yaşamına yön veren kavramlar olarak, özellikle demokratik toplumlarda bilinçli veya farkında olunmadan kitleleri etkilemektedir. Diğer taraftan içinde bulunduğumuz bilgi iletişim çağı ve enformasyon toplumu, bireyleri bu kavramlar karşısında aktif ve seçici konuma getirmektedir. Siyaset, medya ve hedef kitlenin öneminden yola çıkılarak yapılan bu çalışmada, 31 Mart 2019 Mahalli İdareler Genel Seçimleri ile ilgili televizyonda ve dijital medyada yayınlanan Ak Parti seçim reklamları analiz edilmiştir. Bu doğrultuda, İngiliz Kültürel Çalışmaları geleneğinden Stuart Hall’ün kodlama ve kodaçımlama tekniği ile öne sürdüğü alımlama analizi uygulanmıştır. Kamuoyu oluşturmak için yapılan seçim reklamlarının, hedef kitle tarafından nasıl bir okuma biçimiyle alımlandığı, 10 üniversite öğrencisi üzerinde yapılan derinlemesine mülakatla çözümlenmeye çalışılmıştır. Çalışmanın sonucunda; hedef kitle tarafından büyük oranda egemen okuma yapıldığı, müzakereli ve karşıt okumalarla verilen cevapların da anlamlı sayılabilecek bir seviyede çıktığı görülmüştür. Diğer taraftan hedef kitlenin politik katılımında ve oy verme davranışında siyasi reklamların olumlu veya olumsuz bir etkide bulunduğu ancak hedef kitleleri yönlendirmede tek başına yetersiz olduğu sonucuna ulaşılmıştır.
Hypolipidemic Effects and Mechanisms of Val-Phe-Val-Arg-Asn in C57BL/6J Mice and 3T3-L1 Cell Models
Authors: W Shi , Hui He , Ruge Cao , Tao Hou
Published: September 2019

Val-Phe-Val-Arg-Asn (VFVRN) has been identified and screened from lipid-lowering chickpea peptides (ChPs) by using a pharmacokinetic model in our previous experiment. The present study was conducted to investigate its effects and mechanisms on lipid metabolism. A high-fat diet C57BL/6J mice model and 3T3-L1 preadipocyte cell model were used. VFVRN was found to significantly decrease the levels of some blood lipids. The expressions of LDL receptor (LDLR), peroxisome proliferator-activated receptors (PPAR)α, liver X receptor (LXR)α, cholesterol 7α-hydroxylase (CYP7A1) and AMP-activated protein kinase (p-AMPK) in liver were up-regulated by VFVRN treatment. The expressions of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR), fatty acid synthetase (FAS), 1-aminocyclopropane-1-carboxylate synthetase (ACC), sterol regulatory element-binding protein (SREBP)-1c and SREBP-2 in liver were significantly (P<0.05) down-regulated. Additionally, the expressions of PPARα and PPARγ in adipose tissues were up-regulated by VFVRN significantly (P<0.05). VFVRN might also contribute to transintestinal cholesterol efflux (TICE) by up-regulating the expressions of LXRα and ATP binding cassette G5/8 transporters (ABGC5/8). Moreover, VFVRN promoted 3T3-L1 preadipocyte apoptosis by up-regulating the expressions of BaX, cleaved Caspase-3 and down-regulating Bcl-2. VFVRN had potent effects in reversing metabolic disorders of blood and liver in a high-fat diet mice model, as well as to promote the apoptosis of 3T3-L1 preadipocytes.
Potential of Advanced Consolidants for the Application on Sandstone Rocks: A Comparative Study
Authors: Remzova Monika , Zouzelka Radek , Lukes Jaroslav , Rathousky Jiri
Published: September 2019

To achieve efficient and durable consolidation of weathered sandstone, the selection of a suitable consolidant is essential. To reasonably assess the suitability of different formulations, it is fundamental to compare their performance as a consolidant within a substrate, which reliably models the properties of deteriorated material. As a test substrate, the sandstone from quarries in Mšené in central Bohemia was selected, for its developed porosity and relatively low mechanical strength. To obtain relevant comparison of their application potential, both commercial (Remmers KSE OH and Surfapore) and self-developed consolidants were included. To test the long-term stability of each consolidant, the stone was subjected to accelerated weathering. The characterization of texture properties was based on the physical sorption of nitrogen and krypton, mercury intrusion porosimetry and water uptake. While the mechanical properties in microscale were determined by nanoindentation, the mechanical strength in macroscale before and after consolidation was measured by drilling resistance. Both commercial exhibited good mechanical performance with reasonable durability. The performance of our developed samples was comparable or, in some cases, superior. Very interesting were the consolidants containing TiO2 and ZnO nanoparticles, the former exhibiting comparable degree of consolidation and durability as commercial ones, with additional photocatalytic function, the latter unusually high increase in the mechanical strength, even after the weathering test. The diammonium hydrogen phosphate based consolidant showed exceptional durability in the weathering test, which makes it a promising product not only for carbonate but also sandstone materials.
Water, Livelihoods and Migration in SIDS: Climate Change and Future Prospects for Carriacou, West Indies
Authors: Cashman Adrian , Yawson David
Published: September 2019

Caribbean SIDS are among the most vulnerable to climate change which will have a disproportionate impact on local environments and economies. Whilst there is a growing literature on how Caribbean SIDS can adapt to become more resilient a question that has received little attention is with regard to migration as an unplanned response. It is recognised that events such as hurricanes and flooding can lead to internal relocation in the short term but societal responses to droughts through migration have not generally been investigated. This paper seeks to address this by considering the case of the island of Carriacou, part of the state of Grenada. Carriacou with its small population, limited land area and local economy, historically based on agriculture has had a high degree of migration. This is in part a response to limited economic opportunities. Environmental stress manifest through limited water availability, inappropriate land management and social conditions is likely to be exacerbated by climate change and variability. Resultant increases in the frequency and intensity of droughts, in the absence of proactive interventions, are likely to result in non-linear migration, both to Grenada itself and beyond.
Comparative Analysis of Compiler Performances and Program Efficiency
Authors: Bukie Paul Tawo , Udeze Chinedu Leonard , Obono Iwara Ofem , Edim Edim Bassey
Published: September 2019

With the existence of several programming languages such as C/C++, Java, C#, LISP, Prolog, Python, Simula, F#, Go, Haskell, Scala, Ruby, Dart, Swift, Groovy etc. and diverse paradigms like structured, object-oriented, list, aspect-oriented, service-oriented, web, mobile and logic programming, there is a need to perform an exhaustive comparative analysis of diverse compilers and environments before making a choice of implementation technology in software engineering. Optimization of compilers helps to reduce execution time by making use of high speed processor registers, thereby, eliminating redundant computation. This paper reports some series of performance analysis done with some popular programming languages including Java, C++, Python and PHP. Programs involving recursive and iterative functions like factorial of large numbers and binary search of large arrays were run on the various platforms with the execution time recorded in milliseconds and represented in a chart. This can aid in making a selection of the appropriate language to use for a given application domain.
The Multilevel Structure of Sulfonated Syndiotactic-Polystyrene Model Polyelectrolyte Membranes Resolved by Extended Q-range Contrast Variation SANS
Authors: Schiavone Maria-Maddalena , Iwase Hiroki , Takata Shin-ichi , Radulescu Aurel
Published: September 2019

Membranes based on sulfonated synditoactic polystyrene were thoroughly characterized by contrast variation SANS over a wide Q-range in dry and hydrated states. The film samples were prepared by solid-state sulfonation that allowed a uniform sulfonation of only the amorphous phase while preserving the crystallinity of the membrane. The samples were loaded with different guest molecules in either the amorphous (fullerenes) or the crystalline (toluene) regions, in order to vary the neutron contrast or to reproduce the conditions enabling an increased resistance of the membranes to chemical decomposition. The use of uni-axially deformed film samples and contrast variation with different H2O/D2O mixtures allowed for the identification and characterization of different structural levels with sizes between nm and μm, which form and evolve in the membrane morphology in dry and hydrated states and produce scattering features on different detection sectors and at different detection distances after the sample, depending on their size and orientation.
β-HPV 8E6 Attenuates ATM and ATR Signaling in Response to UV Damage
Authors: Snow Jazmine A. , Murthy Vaibhav , Dacus Dalton , Hu Changkun , Wallace Nicholas A.
Published: September 2019

Given the high prevalence of cutaneous genus beta human papillomavirus (β-HPV) infections, it is important to understand how they are manipulating their host cells. This is particularly true for cellular responses to UV damage, since our skin is continually exposed to UV. The E6 protein from β-HPV (β-HPV E6) decreases the abundance of two essential UV-repair kinases (ATM and ATR). Since β-HPV E6 reduces their availability, the impact on downstream signaling events has been uncertain. We demonstrate that β-HPV E6 decreases ATM and ATR activation. This inhibition extended to XPA, an ATR target necessary for UV repair, lowering both its phosphorylation and accumulation. β-HPV E6 hinders POLη phosphorylation and foci formation, critical steps in translesion synthesis. ATM’s phosphorylation of BRCA1 is also attenuated by β-HPV E6. However, β-HPV E6’s hindrance of ATM/ATR signaling during UV-associated cell cycle arrest was incomplete. While there was less phosphorylation of immediate downstream targets (CHK1), events further down the cascade were not decreased. These observations are consistent with β-HPV infections making UV radiation more deleterious and support the proposed role of β-HPV in early stages of non-melanoma skin cancer development.
Optimal Network Reconfiguration in Active Distribution Networks with Soft Open Points and Distributed Generation
Authors: Diaaeldin Ibrahim M. , Abdel Aleem Shady H. E. , El-Rafei Ahmed , Abdelaziz Almoataz Y. , Zobaa Ahmed F.
Published: September 2019

In this paper, a recent meta-heuristic optimization algorithm called the discrete-continuous hyper-spherical search algorithm is used to solve the mixed-integer nonlinear problem of soft open points (SOPs) and renewable distributed generators allocation along with new network reconfiguration methodology under different loading conditions to minimize the total power loss in balanced distribution systems. Multi-scenario studies, which aim to improve the investigation of the overall performance of the strategies, are conducted on IEEE 33-node and 83-node balanced distribution systems. The contributions of SOP losses to the total active losses, as well as the effect of increasing the number of SOPs connected to the system, are investigated to determine the real benefits gained from their allocation. The results obtained validate, with proper justifications, the effectiveness of allocating both SOPs and renewable distributed generators with the proposed network reconfiguration methodology to provide the best operation of distribution networks with minimum losses and enhanced power quality performance. It was also shown that SOPs successfully assist the growing integration plans of the renewable distributed generators units and can address issues related to voltage violations and network losses efficiently.
Efektivitas Tumpangsari Kunyit terhadap Penekanan Nematoda Sista Kuning pada Kentang
Authors: Munawaroh Dini , Subagiya Subagiya , Poromarto Susilo Hambeg
Published: September 2019

Golden cyst nematode is one of the important pests that causes potatoes loss yield. Globodera rostochiensis is the pest that difficult to control because it can withstand in adverse environmental conditions. In Indonesia, golden cyst nematode was first discovered in 2003 in East Java. One of the methods that used in this research is intercropping system. The main objectives in this study was to examine how the effect of turmeric plants on intercropping system to cysts population and amount of eggs on golden cyst nematode and the use of turmeric plants is expected to reduce the application of nematicides. This research was conducted on November 2017-February 2018, in Dieng Banjarnegara. The results show the effect of turmeric on intercropping system had not been able to control the cysts population through the eggs, beside that the eggs are not decrease due to genetic factors of each nematode and environment that effect on eggs formation. But the balancing of use turmeric plants on intercropping system could reduce the cysts population. It can be seen on K1P1 (1 Potato : 1 Turmeric) treatment that showed of decreases of cysts population compared to control treatment. The difference on cysts population is caused by turmeric root exudates.
Sintesis 3,4,4’-Trimetoksikalkon dan Karakterisasinya
Authors: Rehana Rehana , Fahreza Muhammad Salman , S Masita Wulandari
Published: September 2019

Senyawa 3,4,4’-trimetoksikalkon dapat disintesis dari 4-metoksi asetofenon dan 3,4-dimetoksi benzaldehid melalui reaksi Claisen-Schmidt. Reaksi Claisen-Schmidt dipengaruhi jumlah katalis basa dan lama reaksi. Penelitian ini bertujuan untuk mengetahui variasi waktu reaksi dan jumlah NaOH terhadap rendemen 3,4,4’-trimetoksikalkon. Penelitian ini meliputi sintesis 10 mmol 3,4,4’-trimetoksikalkon dari 10 mmol 4-metoksi asetofenon dan 3,4-dimetoksi benzaldehid dengan jumlah katalis NaOH 2, 4, 8, 12, 16 dan 20 mmol dengan waktu reaksi pembentukan enolat 1, 2, 3, 4 dan 5 jam. Hasil sintesis diuji kemuriannya dengan Kromatografi Lapis Tipis (KLT) dan titik lebur kemudian dikarakterisasi dengan spektrofotometri UV-visible dan Nuclear Magnetic Resonance (NMR). Peningkatan konsentrasi NaOH dari 2, 4 dan 8 mmol meningkatkan rendemen hasil sintesis dari 60,06%; 62,60% dan 80,19%, tetapi penambahan NaOH di atas 8 mmol menurunkan rendemen. Peningkatan waktu reaksi dari 1, 2 dan 3 jam meningkatkan rendemen dari 74,20%; 78,61% dan 87,66 %, tetapi penambahan waktu 4 dan 5 jam menurunkan rendemen. Analisis KLT menunjukkan bahwa hasil sintesis murni dengan titik lebur 82 – 85 oC. Karakterisasi menggunakan UV-visibel dan NMR menunjukkan senyawa hasil sintesis adalah 3,4,4’-trimetoksikalkon. Konsentrasi NaOH dan lama waktu reaksi pembentukan enolat mempengaruhi rendemen sintesis 3,4,4’-trimetoksikalkon dengan rendemen terbesar menggunakan katalis NaOH 8 mmol dengan waktu reaksi pembentukan enolat selama 3 jam.
Komposit Polianilina/Kitosan/Perak Nanowires Sebagai Elektrokatalis Reaksi Evolusi Hidrogen dalam Medium Netral
Authors: Fakhrullah Fadli , Sugita Purwantiningsih , Khotib Mohammad , Akiyoshi Takeno , Takahashi Shinya
Published: September 2019

Studi mengenai elektrokatalis untuk reaksi evolusi hidrogen (HER) dan evolusi oksigen (OER) sangat diperlukan terkait aplikasinya dalam pengembangan energi terbarukan berbasis hidrogen. Komposit perak nanowires (AgNWs) dengan polianilina (PANI) disiapkan dengan metode polimerisasi radikal dalam larutan matriks kitosan untuk menurunkan ukuran partikel PANI. Komposit disintesis dengan 3 perlakuan yang berbeda, yaitu perlakuan suhu ruang, suhu rendah, dan sonikasi. Aktivitas elektrokatalisis diukur dengan voltammetri sapuan linear (LSV). Informasi tentang densitas arus pertukaran dan overpotensial sebagai variabel utama pengukuran aktivitas elektrokatalisis diperoleh dari plot Tafel pada LSV. Semua komposit dengan perlakuan suhu dan sonikasi menunjukkan karakter katodik berdasarkan nilai overpotensialnya yang negatif (aktivitas HER), sedangkan komposit dengan perlakuan suhu rendah memiliki karakter anodik (aktivitas OER).

A Composite of   Polyaniline/Chitosan/Silver Nanowires as Hydrogen Evolution Electrocatalyst in Neutral Medium. Study on electrocatalyst for hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) is urgently needed related to its application in hydrogen-based renewable energy development. A composite of silver nanowires (AgNWs) with polyaniline (PANI) was prepared by radical polymerization method in chitosan matrix solution to reduce the PANI particle size. Each composite was synthesized under three different treatment conditions, which are room temperature, low temperature, and sonication. Linear sweeping voltammetry (LSV) was conducted to measure electrocatalytic activity of the composites. Information about exchange current density and overpotential as important variables in this field were obtained from Tafel plot in the LSV. The temperature and sonication-treated composites have cathodic character due to its negative overpotential (HER activity), while low temperature-treated composites have anodic character (OER activity).
The Effect of Tiller Transplanting Technique and Water Availability on the Growth and Yield of Sidaguri (Sida rhombifolia)
Authors: Putra Dhimas Taufika , Pujiasmanto Bambang , Supriyono Supriyono
Published: September 2019

Sidaguri (Sida rhombifolia) is a wild plant with the ability to cure various diseases. Continuous exploitation of natural source can reduce the sustainability of this plant species. On the other hand, this plant is often ignored because it is considered as weed. This study aims to provide information about proper cultivation of Sidaguri to the community and medicinal plant farmers in order to maintain its sustainability and its potential to be used as cultivated plant. The study used a Completely Randomized Design (CRD) with two treatment factors: transplanting techniques (puteran, cabutan, stump) and water availability (50, 37.5, 25, 12.5% water field capacity). Observation data were analyzed by analysis of variance and followed by DMRT test at 5% upon the significant result. The results have revealed that the transplanting technique of transplanting tillers with puteran technique produces the best growth and 25% water availability increases plant growth and yield. The result suggests that Sidaguri is a plant that is tolerant to drought.
An Analysis of Wheat Farming: Calculations and Perceptions
Authors: Zebua Damara Dinda Nirmalasari , Priyanto Sony Heru , Sunaryanto Lasmono Tri
Published: September 2019

National diversification of wheat-based food has been put into practice. Public consumption of flour-based food, such as noodles and bread, is in great demand. This study aims to determine the calculations, perceptions and attitudes of farmers towards wheat farming, considering that Indonesia is a non-wheat developing country. This qualitative descriptive research using in-depth interviews with 7 informants and observation methods was conducted in Klaten and Demak Regencies. In analyzing the data, this study used an interactive analysis model including data reduction, data presentation and conclusion drawing. The results show that: 1) based on farmers' calculations, wheat farming is financially not profitable to farmers because the products harvested are not worth the expense; 2) farmers' calculations affect farmers' perceptions which shifted to negative perceptions that wheat farming is not as promising as other farming businesses. Despite the negative perceptions, wheat farming has provided a valuable experience for farmers which is shown by their pride and pleasure and 3) the majority of farmers are not interested in wheat farming, which is shown by their attitude of not being able to accept wheat farming, while other farmers behave otherwise, indicated by their willingness to recommence wheat planting testing. Further research may examine: 1) how to change the culture of rice farming into culture of wheat farming; 2) how to change subsistence rice farming into enterprise and 3) how to change rice farming as social activities to rice farming as business activities.
Effects of Planting Time and Cultivar on Leaf Physiology and Seed Yield of Soybean (Glycine max. (L.) Merr)
Authors: Srihartanto Eko , Indradewa Didik
Published: September 2019

The use of adaptive soybean cultivars with appropriate planting time on dry land can enhance the improvement of soybean growth and yield. This study aimed to determine changes in leaf physiological character and soybean yield as affected by different planting time and superior soybean cultivars. The experiment was conducted on the dry land of Inceptisol in Gunungkidul from November 2018 to March 2019. The experiment was arranged in a Split Plot Design with the main plot arranged in a Randomized Complete Block Design (RCBD) with three (3) replications. The main plots consisted of three planting times, i.e. early planting time–Pranoto Mongso, midldle planting time–Farmer Method and late planting–Katam Terpadu. The subplots consisted of four soybean cultivars, namely Anjasmoro, Argomulyo, Grobogan and Dega-1. The fertilizers used were organic fertilizer (2 t ha-1), Urea (50 kg ha-1) and KCl (50 kg ha-1). The results showed that the treatment of planting time and cultivar could increase leaf physiological activity, particularly the number of leaves, total leaf area, leaf area index and plant growth rate. Early planting time–Pranoto Mongso and middle planting time–Farmers Method produced higher plant dry weight (20.11 g; 24.21 g) and seed yields per plant (29.11 g; 26.75 g) than late planting (Katam Terpadu) did (20.30 g). Meanwhile, cultivar Dega-1 had higher seed yields per plant (30.11 g) than cultivar Grobogan (28.39 g), Argomulyo (23.35 g) and Anjasmoro (19.79 g) did.
Investigation of a deadweight force standard machine with a 1 mN – 10 N range
Authors: Hu Gang , Jiang Jile , Li Tao , Zhang Zhimin , Ji Honglei , Zhuang Lijun
Published: September 2019

A 10 N small force standard machine with air-bearing support has been developed by NIM. Differing from traditional deadweight force standard machines, this machine is equipped with a balance beam supported by air bearing. The weights of the load frame and deadweight hanging system are balanced, and the initial force is extended to 1 mN. Furthermore, there are innovative designs in the load frame and tension force joint. Performance experiments such as sensitivity, repeatability, and rotation effect are carried out. The uncertainty of this machine is evaluated as 1.0×10-4 (k = 2) in the range of 1 mN – 0.5 N, 4.0 × 10-5 (k = 2) in the range of 0.5 N – 10 N. The structure of this force standard machine and its key components are introduced in detail. The performance experimental results and uncertainty evaluation are demonstrated in this paper.
Different long-term characteristics of hydraulic pressure gauges under constant pressure applications
Authors: Kajikawa Hiroaki , Kobata Tokihiko
Published: September 2019

This article focuses on the long-term drifts of hydraulic pressure gauges, which are constantly subjected to high pressures. A target measurement pressure was consistently applied to pressure gauges for more than two months, and the gauges were occasionally calibrated against a reference pressure balance. The long-term drift has been evaluated based on the variations in calibration values with the elapsed time since the pressure application. We evaluated the long-term drift of three kinds of pressure gauges that are commonly used for calibrations and industrial measurements. The long-term drift differed depending on the type of sensing structure. Although the gauges that had similar sensing structures showed similar drift when the gauges were pressurised due to atmospheric pressure, the amount of the drift depended on each product. In addition, the long-term drift was also affected significantly by the preliminary pressure conditions, even under the same pressure application. Preliminary or ex-post evaluations are necessary for the respective gauges under similar pressure conditions. In practice, the drift of zero-reading is often the main contributory factor of the drift; therefore, the drift can be mitigated by tracing the zero-reading during the pressure applications
Establishment of torque realisation up to 5 kN·m with a new design of the torque standard machine
Authors: Arksonnarong Nittaya , Saenkhum Nattapon , Chantaraksa Pramann , Sanponpute Tassanai
Published: September 2019

A Torque Standard Machine (TSM) with a rated capacity of 5 kN·m was designed and constructed by the Torque Laboratory, National Institute of Metrology (Thailand), NIMT. The machine had initially used a flexure bearing as a fulcrum. It had been developed based on the research of a 10 N·m suspended fulcrum TSM. However, the bearing structure was changed to a combination of eight elastic hinges in order to withstand larger cross-forces for providing greater strength and providing a shorter stabilising time, consuming the lever arm’s swing. With a three-column weightlifting system, the machine provides five measuring ranges ranging from 100 N·m to 5,000 N·m in the same set of stacked weights.

The measurement results showed the sensitivity of the fulcrum within ± 0.005 N·m from 10 % to 100 % of the measurement range. The sensitivity of the fulcrum is one of the main sources of the uncertainty evaluation of the torque measurement. The Calibration and Measurement Capabilities (CMCs) of the torque measurement were 0.01 % (k=2) in the measurement range from 500 N·m to 5,000 N·m. To confirm the capability of the measurement, an informal comparison with Physikalisch-Technische Bundesanstalt (PTB) was conducted. The results were satisfactory, with the |En| less than 1.
Pengaruh Non Performing Loan Dan Biaya Oprasional Pendapatan Operasional Terhadap Laba Bersih
Authors: Wida Oda , Zakaria Hakim Heikal Muhammad , Huda Syamsul
Published: September 2019

The purpose of this study was to analyze the effect of Operational Income Opportunity (BOPO), Non Performing Loans (NPL) on Net Income of 10 conventional banks that have the highest assets according to the Financial Services Authority (OJK) in 2017. The results showed that Non Performing Loan does not have a significant effect on net income, BOPO has an effect on net income. Based on the results of Determination Coefficient Analysis, the independent variables can explain the effect on Net Profit of 55.5%, while the remainder is explained by other variables not explained in this study. Based on the results of the F test, the independent variables simultaneously have a significant effect on Net Profit.

 
Penerapan Akuntansi Persediaan Barang Dagang pada UD Prapatan Motor Kudus
Authors: Rakhmawati Ita
Published: September 2019

Merchandise inventory is one account that has significant values and functions. The amount of inventory must not be too much because it will affect to liquidity. However, it should not be too small because it will eliminate potential of revenue from customers. This research aim to whether the accounting treatment of good inventory methods on Prapatan Motor Entreprise. This research uses qualitative approach, The types of data collected are primary and secondary data. This study related of recording, assessment, and reporting of finished goods inventory . This study concludes Prapatan Motor Entreprise have execute an accounting inventory process. The company use physical recording methods and average methods on inventory assesment.on the end of accounting period, Inventory present on financial position and cost of production report. But the company don’t have inventory control card so must stock opname periodically.

 
Application of Fluorescent Markers to Determine the Formation Fluid Inflow After MFrac
Authors: Guryanov Andrey , Gazizov Ruslan , Medvedev Evgeny , Ovchinnikov Kirill , Buzin Pavel , Katashov Alexander
Published: September 2019

The primary objective of the technical development and underlying principles described in this article is the creation of physicochemical principles, of which the practical implementation allows users to quickly and accurately conduct production logging of horizontal wells after conducting multi-stage hydraulic fracturing.

The main physical phenomenon applied in the described method is the fluorescence of polymer microspheres – marker-reporters ranging in size from several hundred nanometers to several microns and containing quantum dots. Marker-reporters pass from synthesis and injection of proppant/sand into the polymer shell at our company's production facilities to high-precision instrumental determination of their concentration in formation fluid samples using flow cytofluorometry method in the laboratory. This method includes the following stages:

Synthesis of marker-reporters containing quantum dots Preparation of polymer-coated proppant / sand with markers Injection of the marked polymer-coated proppant / sand into the well during MFrac, followed by formation fluid filtration through it Formation fluid sampling Sample preparation for obtaining samples to be analyzed with the flow cytometer Determination of marker-reporter concentrations in the samples by flow cytofluorometry data processing, also with our corporate software based on machine- learning principles

All the stages mentioned above are constantly being improved and optimized. The description of each stage of the relevant technological process is described below with a "historical reference" to the technological development behind it. The characteristics of the marked polymer-coated proppant Geosplit are also provided herein.
Study of the Prospects for Gas-Containing Terrigenous Deposits of the Middle and Lower Jurassic on One of the Areas within the Afghan-Tajik Depression
Authors: Tillyabaev Muzafar , Abidov Khurshid , Klevitskiy Alexandr , Levin Dmitry , Shamkolovich Galina
Published: September 2019

Given the current degree of exploration maturity within the Afghan-Tajik Depression, much of which lies within the southeastern Republic of Uzbekistan, there is a vital need to conduct geological studies of the Lower to Middle Jurassic strata and to elaborate a geological exploration program that will achieve the most comprehensive substantiation of hydrocarbon potential associated with these sediments. Since none of the prospecting and appraisal wells has reached the deep-seated Lower to Middle Jurassic sediments in the region of interest, an in-depth study of available seismic data aimed at estimating their hydrocarbon potential becomes a matter of high priority. This paper discusses the studies conducted in one of 3D seismic areas on the Lower to Middle Jurassic sediments. Porosity data from analogous fields were statistically analysed for the purpose of estimating hydrocarbon resources. This step helped substantiate volumetric parameters even using extremely scarce actual information. The completed studies have resulted in recommendations for drilling prospecting wells into the target Lower to Middle Jurassic strata as well as predicting the depth of burial at which producing sandstones occur.
Transcriptomic and evolutionary analysis of the mechanisms by which P. argentatum, a rubber producing perennial, responds to drought
Authors: Nelson Andrew D. L. , Ponciano Grisel , McMahan Colleen , Ilut Daniel C. , Pugh N. Ace , Elshikha Diaa Eldin , Hunsaker Douglas J. , Pauli Duke
Published: September 2019

Background Guayule (Parthenium argentatum Gray) is a drought tolerant, rubber producing perennial shrub native to northern Mexico and the US Southwest. Hevea brasiliensis, currently the world’s only source of natural rubber, is grown as a monoculture, leaving it vulnerable to both biotic and abiotic stressors. Isolation of rubber from guayule occurs by mechanical harvesting of the entire plant. It has been reported that environmental conditions leading up to harvest have a profound impact on rubber yield. The link between rubber biosynthesis and drought, a common environmental condition in guayule’s native habitat, is currently unclear. Results We took a transcriptomic and comparative genomic approach to determine how drought impacts rubber biosynthesis in guayule. We compared transcriptional profiles of stem tissue, the location of guayule rubber biosynthesis, collected from field-grown plants subjected to water-deficit (drought) and well-watered (control) conditions. Plants subjected to the imposed drought conditions displayed an increase in production of transcripts associated with defense responses and water homeostasis, and a decrease in transcripts associated with rubber biosynthesis. An evolutionary and comparative analysis of stress-response transcripts suggests that more anciently duplicated transcripts shared among the Asteraceae, rather than recently derived duplicates, are contributing to the drought response observed in guayule. In addition, we identified several deeply conserved long non-coding RNAs (lncRNAs) containing microRNA binding motifs. One lncRNA in particular, with origins at the base of Asteraceae, may be regulating the vegetative to reproductive transition observed in water-stressed guayule by acting as a miRNA sponge for miR166. Conclusions These data represent the first genomic analyses of how guayule responds to drought like conditions in agricultural production settings. We identified an inverse relationship between stress-responsive transcripts and those associated with precursor pathways to rubber biosynthesis suggesting a physiological trade-off between maintaining homeostasis and plant productivity. We also identify a number of regulators of abiotic responses, including transcription factors and lncRNAs, that are strong candidates for future projects aimed at modulating rubber biosynthesis under water-limiting conditions common to guayules’ native production environment.
Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris
Authors: Conacher Cleo G. , García-Aparicio María , Coetzee Gerhardt , Zyl W.H. (Emile) van , Görgens Johann F.
Published: September 2019

Objective: Glucuronoyl esterase (GE) is an emerging enzyme that improves fractionation of lignin-carbohydrate complexes. However, the commercial availability of GE is limited, which hinders the research of GE-based bioprocesses for its industrial application in lignocellulose biorefineries. This study evaluated a workable, cost-effective, and commercially scalable production strategy to improve the ease of GE-based research. This strategy consisted of a constitutive and methanol-free enzyme production step coupled with a two-step filtration process. The aim was to determine if this strategy can yield copious amounts of GE, by secretion into the extracellular medium with an acceptable purity that could allow its direct application. This approach was further validated for cellobiose dehydrogenase, another emerging lignocellulose degrading enzyme which is scarcely available at high cost. Results: The secreted recombinant enzymes were functionally produced in excess of levels previously reported for constitutive production (1489-2780 mg.L -1 ), and were secreted at moderate to high percentages of the total extracellular protein (51-94 %). The constant glycerol feed, implemented during fed-batch fermentation, lead to a decline in growth rate and plateaued productivity. Tangential flow ultrafiltration was used to concentrate cell-free enzyme extracts 5-6-fold, reaching enzyme activity levels (1020-202 U.L -1 ) that could allow their direct application.
Comparative analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle, and buffalo
Authors: Saleh Ahmed Abdel-kader , Rashad Amr M.A. , Hassanine Nada. N.A.M. , Sharaby Mahmoud A. , Zhao Yongju
Published: September 2019

Objective: A total of 205 animals from four Egyptian livestock species; cattle (n=18), buffaloes (n=12), sheep (n=150) and goats (n=25) were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR-RFLP).
Results: The amplified fragments were found to be of length 654 bp in sheep, 651bp in cattle and 655 bp in buffalo. For Falahy goats, PCR was performed to amplify a 316 bp fragment from exon 2 of the IGFBP-3 gene. The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern for goats, while for cattle, 3 genotypes were identified; (AA),(AB), and (BB). Moreover, for buffalo one genotype (AA) only was found with HaeIII and TaqI restriction enzymes, separately. Also, the digestion profile for goats with HaeIII revealed one pattern only. Nucleotide sequencing of the amplified fragments of IGFBP-3 gene in sheep, cattle, buffalo, and goat was submitted to the NCBI GenBank (Accession no. MG738671.1, MG738673.1, MG738674.1, and MG738672.1, respectively). The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 88.54, 89.63 and 95.06 % between ''sheep and cattle '', ''sheep and buffalo '', and ''cattle and buffalo'', respectively.
A protocol for Epon-embedding-based correlative super-resolution light and electron microscopy
Authors: Xu Pingyong , Xu Tao , Zhang Mingshu , Fu Zhifei , Peng Dingming , Xue Fudong , Zhang Rui , He Wenting
Published: September 2019

This protocol describes a detailed step-by-step sample preparation procedure for Epon based post-embedding correlative super-resolution light and electron microscopy (SR-CLEM). The newly developed fluorescent protein, mEosEM, is the core in this protocol. The advantage of this method is to simultaneously obtain high-quality LM and EM images from the same ultrathin section of Epon-embedded sample after conventional chemical fixation.
Powdery and Downy mildew infections regulates a cascade of defense-responsive genes in Vitis Vinifera
Authors: Goyal Neetu , Bhatia Garima , Garewal Naina , Upadhyay Anuradha , Singh Kashmir
Published: September 2019

Background: Grape cultivation is severely affected by powdery mildew and downy mildew worldwide leading to significant losses in productivity and quality. To control such diseases in an environment friendly way, it is essential to understand the molecular mechanisms of fungal resistance in grapes. When the plant is infected with pathogen, various defense mechanisms are activated in plants at molecular level, the ultimate aim of all is to provide immunity against infection. Results: In our study, we tried to understand the Salicylic acid (SA) mediated pathway of ETI through a cascade of defense molecules ultimately leading to activation and expression of PR proteins that provides SAR in plants. We performed genome-wide identification of defensive molecules expressed during PM and DM infection in grapevine. Consequently, we identified 2, 4, 7, 4, 2, 1, 20 and 7 differentially expressed PM-responsive defensive genes ( NBS-LRR, EDS1, NDR1, NPR, PAD4, RAR1, TFs and PR ) and 28, 2, 5, 4, 1, 39 and 19 differentially expressed DM-responsive defensive genes ( NBS-LRR, EDS1, NDR1, NPR, RAR1, TFs and PR ) in V. vinifera. Next, the co-expression between the identified defensive genes was performed that tells us about the sequence of events that occurs during a signaling cascade in response to PM and DM-infection. Further, we characterized the identified genes on the basis of physico-chemical properties, chromosomal positions, gene and protein structure analysis and functional annotation. Conclusion: The powdery and downy mildew responsive defensive genes identified in this study can be used to develop new and improved PM and DM-resistant grape varieties in future.
Factors Affecting the Implementation of Continuous Quality Improvement in Health Facilities of Southern Nation and Nationalities People Region (SNNPR)
Authors: Yeshanew Addisu Gize , Wendwessen Nebiyou , Teklemariam Dereje
Published: September 2019

Continuous Quality improvement has become an important aspect of healthcare organizations. The objective of this study was to identify contributing factors for implementing continuous quality improvement projects in health facilities for the better delivery of health care service in the case of south nation and nationalities people, Ethiopia. Cross-sectional study was conducted from April to May 2018 in SNNPR of two district woredas health facilities. Descriptive, bivariate and multivariate analysis used, during the analysis, 0.05 p-value and 95% confidence interval (CI) was used to judge the significance of the associations. So that p-value less than 0.05 were taken as significant association. The finding of this study showed that, a total of 144 health professionals were participated, giving a response rate of 100%. Majority of the respondents (75%) were male and 35% said that their respective health facility implemented continuous quality improvement project. More than halve of (51.4%) the leaders were not receptive for new ideas; moreover, majority of leader were not encouraging learning (62.5%) and not engaged in quality improvement project implementation process (66%). Variables like leaders receptive to new ideas, leaders share information/ data about health facility service delivery status, health facility has a quality improvement project plan, staff know using indicators to tell progress about service delivery, health facility assess client satisfaction level, were the independent predictors of continuous quality improvement(CQI) project implementation.
Evaluation and comparison of six GRACE models to stratify undifferentiated chest pain at the emergency department
Authors: Zheng Wen , Wang Guangmei , Ma Jingjing , Wu Shuo , Zhang He , Zheng Jiaqi , Xu Feng , Wang Jiali , Chen Yuguo
Published: September 2019

Background

The Global Registry of Acute Coronary Events (GRACE) score is recommended for stratifying chest pain. However, there are six formulas used to calculate the GRACE score for different outcomes of acute coronary syndrome (ACS), including death (Dth) or composite of death and myocardial infarction (MI), during in hospital (IH), in 6 months after discharge (OH6m) or from admission to 6 months later (IH6m). The more appropriate one for stratification of undifferentiated chest pain remains unclear. We aimed to provide firstly comprehensive evaluation and comparison of six GRACE models to predict 30-day major adverse cardiac events (MACE) in acute chest pain at the emergency department (ED).

Methods

Patients with acute chest pain were consecutively recruited from August 24, 2015 to September 30, 2017 in EDs of two public hospitals in China. The primary outcome was MACE within 30 days, including death, acute myocardial infarction (MI), emergency revascularization, cardiac arrest and cardiogenic shock. GRACE scores were calculated retrospectively using the prospectively obtained data. Correlation, calibration, discrimination and reclassification of six GRACE models were evaluated.

Results

A total of 2886 patients were analyzed, with 590 (20.4%) patients getting outcomes. The GRACE (IH6mDthMI), GRACE (IHDthMI), GRACE (IHDth), GRACE (IH6mDth), GRACE (OH6mDth) and GRACE (OH6mDthMI) showed positive linear correlation with actual MACE rates (r≥0.568, p<0.001), first two of which exerted very strong relationships (r>0.9). All these models had good calibration (Hosmer-Lemeshow goodness-of-fit test, p≥0.073) except GRACE (IHDthMI) (p<0.001). The corresponding c-statistics were 0.82(0.81,0.83), 0.83(0.81,0.84), 0.75(0.73,0.76), 0.73(0.72,0.75), 0.72(0.70,0.73) and 0.70(0.68,0.71). Improvement in AUC, NRI and IDI (p<0.001) represented that GRACE (IH6mDthMI) and GRACE (IHDthMI) were superior to other four models in discrimination and reclassification.

Conclusions

The GRACE (IH6mDthMI) and GRACE (IHDthMI) outperformed other GRACE models in discriminating high or low-risk of 30-day MACE in patients with chest pain. The reasonable application of appropriate GRACE models should be recommended on stratification of undifferentiated chest pain presenting to the ED.
Upregulation of pERK and c-JUN by γ-tocotrienol and not α-tocopherol are essential to the differential effect on apoptosis in prostate cancer cells.
Authors: Moore Christine , Palau Victoria , Mahboob Rashid , Lightner Janet , Stone William , Krishnan Koyamangalath
Published: September 2019

Background: α-tocopherol (AT) and γ-tocotrienol (GT3) are vitamin E isoforms considered to have potential chemopreventive properties. AT has been widely studied in vitro and in clinical trials with mixed results. The latest clinical study (SELECT trial) tested AT in prostate cancer patients, determined that AT provided no benefit, and could promote cancer. Conversely, GT3 has shown antineoplastic properties in several in vitro studies, with no clinical studies published to date. GT3 causes apoptosis via upregulation of the JNK pathway; however, inhibition results in a partial block of cell death. We compared side by side the mechanistic differences in these cells in response to AT and GT3.
Methods: The effects of GT3 and AT were studied on androgen sensitive LNCaP and androgen independent PC-3 prostate cancer cells. Their cytotoxic effects were analyzed via MTT and confirmed by metabolic assays. Cellular pathways were studied by immunoblot. Quantitative analysis and the determination of relationships between cell signaling events were analyzed for both agents tested. Non-cancerous prostate RWPE-1 cells were also included as a control.
Results: The RAF/RAS/ERK pathway was significantly activated by GT3 in LNCaP and PC-3 cells but not by AT. This activation is essential for the apoptotic affect by GT3 as demonstrated the complete inhibition of apoptosis by MEK1 inhibitor U0126. Phospho-c-Jun was upregulated by GT3 but not AT. No changes were observed on AKT for either agent, and no release of cytochrome c into the cytoplasm was detected. Caspases 9 and 3 were efficiently activated by GT3 on both cell lines irrespective of androgen sensitivity, but not in cells dosed with AT. Cell viability of non-cancerous RWPE-1 cells was affected neither by GT3 nor AT.

Conclusions:
c-JUN is a recognized master regulator of apoptosis as shown previously in prostate cancer. However, the mechanism of action of GT3 in these cells also include a significant activation of ERK which is essential for the apoptotic effect of GT3. The activation of both, ERK and c-JUN, is required for apoptosis and may suggest a relevant step in ensuring circumvention of mechanisms of resistance related to the constitutive activation of MEK1.