bluuebunny/crossref_metadata_2025 huggingface dataset:
Age-Dependent Differences in the Rate and Symptoms of TIA Mimics in Patients Presenting With a Suspected TIA to a Neurological Emergency Room
Authors: Ippen Franziska Maria , Walter Fabian , Hametner Christian , Gumbinger Christoph , Nagel Simon , Purrucker Jan C. , Mundiyanapurath Sibu
Published: February 2021

Background: Transient ischemic attack (TIA) needs further diagnostic evaluation to prevent future ischemic stroke. However, prophylaxis can be harmful in elderly if the diagnosis is wrong. We aimed at characterizing differences in TIA mimics in younger and older patients to enhance diagnostic accuracy in elderly patients.

Methods: In a dedicated neurological emergency room (nER) of a tertiary care University hospital, patients with transient neurological symptoms suspicious of TIA (<24 h) were retrospectively analyzed regarding their final diagnoses and their symptoms. These parameters were compared between patients aged 18–70 and >70 years using descriptive, univariable, and multivariable statistics.

Results: From November 2018 until August 2019, 386 consecutive patients were included. 271 (70%) had cardiovascular risk factors and all patients received cerebral imaging, mostly CT [376 (97%)]. There was no difference in the rate of diagnosed TIA between the age groups [85 (46%) vs. 58 (39%); p = 0.213].TIA mimics in the elderly were more often internal medicine diseases [35 (19%) vs. 7 (5%); p < 0.001] and epileptic seizures [48 (26%) vs. 24 (16%); p = 0.032] but less often migraine [2 (1%) vs. 20 (13%); p < 0.001]. The most frequent symptoms in all patients were aphasia and dysarthria [107 (28%) and 92 (24%)]. Sensory impairments were less frequent in elderly patients [23 (11%) vs. 54 (30%); p < 0.001]. Impaired consciousness and orientation were independent predictors for TIA mimics (p < 0.001) whereas facial palsy (p < 0.001) motor weakness (p < 0.001), dysarthria (p = 0.022) and sensory impairment (p < 0.001) were independent predictors of TIA.

Conclusion: TIA mimics in elderly patients are more likely to be internal medicine diseases and epilepsy compared to younger patients. Excluding internal medicine diseases seems to be important in elderly patients. Facial palsy, motor weakness, dysarthria and sensory impairment are associated with TIA.
miR-483-3p, Mediated by KLF9, Functions as Tumor Suppressor in Testicular Seminoma via Targeting MMP9
Authors: Zhang Lei , Ruan Yashi , Qin Zhiqiang , Gao Xian , Xu Kai , Shi Xiaokai , Gao Shenglin , Liu Shouyong , Zhu Kai , Wang Wei , Zuo Li , Zhang Lifeng , Zhang Wei
Published: February 2021

Background

Seminoma (SEM) is the most frequent testicular germ cell tumor with a high incidence in young men. The present study aims to explore the function and regulatory mechanism of miR-483-3p in SEM.

Methods

RT-qPCR was performed to investigate miR-483-3p levels in SEM tissues. The effect of miR-483-3p on TCam-2 cells was assessed by CCK-8, colony formation, cell migration, and invasion assays. Luciferase reporter assays were performed to investigate the interaction between miR-483-3p and MMP9, and then the recovery experiments were performed. Moreover, the potential upstream regulator of miR-483-3p was predicted based on JASPAR database.

Results

miR-483-3p was down-regulated in SEM tissues versus paracancerous normal tissues. The expression level of miR-483-3p was significantly associated with tumor stage by RT-qPCR. Functionally, miR-483-3p over-expression suppressed cell growth, migration, and invasion in SEM cell lines. Mechanically, miR-483-3p negatively regulated MMP9 by directly binding to its 3′-UTR. The over-expression of miR-483-3p could reverse the promoting role of MMP9 over-expression on the proliferation, migration, and invasion of TCam-2 cells. Moreover, KLF9 was identified as a potential upstream regulator of miR-483-3p and functions as a tumor suppressor.

Conclusions

In general, our study suggested that miR-483-3p could inhibit the cell growth, migration, and invasion of testicular SEM by targeting MMP9. Moreover, KLF9 is an upstream positive regulator of miR-483-3p and also functions as a tumor suppressor in SEM.
A Review of Cancer Genetics and Genomics Studies in Africa
Authors: Rotimi Solomon O. , Rotimi Oluwakemi A. , Salhia Bodour
Published: February 2021

Cancer is the second leading cause of death globally and is projected to overtake infectious disease as the leading cause of mortality in Africa within the next two decades. Cancer is a group of genomic diseases that presents with intra- and inter-population unique phenotypes, with Black populations having the burden of morbidity and mortality for most types. At large, the prevention and treatment of cancers have been propelled by the understanding of the genetic make-up of the disease of mostly non-African populations. By the same token, there is a wide knowledge gap in understanding the underlying genetic causes of, and genomic alterations associated with, cancer among black Africans. Accordingly, we performed a review of the literature to survey existing studies on cancer genetics/genomics and curated findings pertaining to publications across multiple cancer types conducted on African populations. We used PubMed MeSH terms to retrieve the relevant publications from 1990 to December 2019. The metadata of these publications were extracted using R text mining packages: RISmed and Pubmed.mineR. The data showed that only 0.329% of cancer publications globally were on Africa, and only 0.016% were on cancer genetics/genomics from Africa. Although the most prevalent cancers in Africa are cancers of the breast, cervix, uterus, and prostate, publications representing breast, colorectal, liver, and blood cancers were the most frequent in our review. The most frequently reported cancer genes were BRCA1, BRCA2, and TP53. Next, the genes reported in the reviewed publications’ abstracts were extracted and annotated into three gene ontology classes. Genes in the cellular component class were mostly associated with cell part and organelle part, while those in biological process and molecular function classes were mainly associated with cell process, biological regulation, and binding, and catalytic activity, respectively. Overall, this review highlights the paucity of research on cancer genomics on African populations, identified gaps, and discussed the need for concerted efforts to encourage more research on cancer genomics in Africa.
Network Pharmacology and Molecular Docking on the Molecular Mechanism of Luo-hua-zi-zhu (LHZZ) Granule in the Prevention and Treatment of Bowel Precancerous Lesions
Authors: Guo Cui , Kang Xingdong , Cao Fang , Yang Jian , Xu Yimin , Liu Xiaoqiang , Li Yuan , Ma Xiumei , Fu Xiaoling
Published: February 2021

The Luo-hua-zi-zhu (LHZZ) granule has been widely used for the treatment of colorectal adenoma (CRA), which is a precursor of colorectal cancer (CRC). However, the active components of LUZZ and its mechanism of action against CRA have not yet been elucidated. This study was designed to investigate the effect of LHZZ on CRA and explore its pharmacological mechanisms. First, a total of 24 chemical constituents were identified in the 50% aqueous methanol extract of LHZZ granule based on the mass fragment patterns and mass spectral library using the high resolution UPLC-Q-TOF MS/MS system. Subsequently, based on a network pharmacology study, 16 bioactive compounds and 28 targets of the LHZZ associated with CRA were obtained, forming a compound-target network. Molecular docking tests showed tight docking of these compounds with predicted targeted proteins. The protein–protein interaction (PPI) network identified AKT1, CASP3, TP53 and EGFR as hub targets. The Kyoto Encyclopedia of Genes and Genomes pathway network and pathway-target-compound network revealed that the apoptosis pathway was enriched by multiple signaling pathways and multiple targets, including the hub targets. Finally, the reliability of the core targets was evaluated using molecular docking technology and in vitro studies. Our study indicated that the LHZZ particle has preventive and treatment effect on colorectal adenoma through multi-component, multi-target and multi-pathway.
Serum Glycerophospholipid Profile in Acute Exacerbation of Chronic Obstructive Pulmonary Disease
Authors: Gai Xiaoyan , Guo Chenglin , Zhang Linlin , Zhang Lijiao , Abulikemu Mairipaiti , Wang Juan , Zhou Qingtao , Chen Yahong , Sun Yongchang , Chang Chun
Published: February 2021

Studies have shown that glycerophospholipids are involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). This study adopted targeted metabolomic analysis to investigate the changes in serum glycerophospholipids in acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and their differential expression in patients with different inflammatory subtypes. Patients with AECOPD admitted between January 2015 and December 2017 were enrolled, and their clinical data were collected. The patients’ gender, age, body mass index, and lung function were recorded. Routine blood and induced sputum tests were performed. Liquid chromatography-mass spectrometry was used to detect the serum glycerophospholipid metabolic profiles and to analyze the metabolic profile changes between the acute exacerbation and recovery stages as well as the differences between different inflammatory subtypes. A total of 58 patients were hospitalized for AECOPD, including 49 male patients with a mean age of 74.8 ± 10.0 years. In the metabolic profiles, the expression of lysophosphatidylcholine (LPC) 18:3, lysophosphatidylethanolamine (LPE) 16:1, and phosphatidylinositol (PI) 32:1 was significantly reduced in the acute exacerbation stage compared to the recovery stage (P < 0.05). The three glycerophospholipids were used to plot the receiver operating characteristic curves to predict the acute exacerbation/recovery stage, and the areas under the curves were all above 70%. There were no differential metabolites between the two groups of patients with blood eosinophil percentage (EOS%) ≥2% and <2% at exacerbation. The expression of LPC 18:3, LPE 16:1, and PI 32:1 was significantly reduced in the acute exacerbation stage compared to the recovery stage in the inflammatory subtype with blood EOS <2% (P < 0.05). Abnormalities in the metabolism of glycerophospholipids may be involved in the onset of AECOPD, especially in the non-eosinophilic subtype.
Highly Efficient Leaf Base Protoplast Isolation and Transient Expression Systems for Orchids and Other Important Monocot Crops
Authors: Ren Rui , Gao Jie , Yin Dongmei , Li Kai , Lu Chuqiao , Ahmad Sagheer , Wei Yonglu , Jin Jianpeng , Zhu Genfa , Yang Fengxi
Published: February 2021

Versatile protoplast platforms greatly facilitate the development of modern botany. However, efficient protoplast-based systems are still challenging for numerous horticultural plants and crops. Orchids are globally cultivated ornamental and medicinal monocot plants, but few efficient protoplast isolation and transient expression systems have been developed. In this study, we established a highly efficient orchid protoplast isolation protocol by selecting suitable source materials and optimizing the enzymatic conditions, which required optimal D-mannitol concentrations (0.4–0.6 M) combined with optimal 1.2% cellulose and 0.6% macerozyme, 5 μM of 2-mercaptoethanol and 6 h digestion. Tissue- and organ-specific protoplasts were successfully isolated from young leaves [∼3.22 × 106/g fresh weight (FW)], flower pedicels (∼5.26 × 106/g FW), and young root tips (∼7.66 × 105/g FW) of Cymbidium orchids. This protocol recommends the leaf base tissues (the tender part of young leaves attached to the stem) as better source materials. High yielding viable protoplasts were isolated from the leaf base of Cymbidium (∼2.50 × 107/g FW), Phalaenopsis (1.83 × 107/g FW), Paphiopedilum (1.10 × 107/g FW), Dendrobium (8.21 × 106/g FW), Arundina (3.78 × 106/g FW) orchids, and other economically important monocot crops including maize (Zea mays) (3.25 × 107/g FW) and rice (Oryza sativa) (4.31 × 107/g FW), which showed marked advantages over previous mesophyll protoplast isolation protocols. Leaf base protoplasts of Cymbidium orchids were used for polyethylene glycol (PEG)-mediated transfection, and a transfection efficiency of more than 80% was achieved. This leaf base protoplast system was applied successfully to analyze the CsDELLA-mediated gibberellin signaling in Cymbidium orchids. We investigated the subcellular localization of the CsDELLA-green fluorescent protein fusion and analyzed the role of CsDELLA in the regulation of gibberellin to flowering-related genes via efficient transient overexpression and gene silencing of CsDELLA in Cymbidium protoplasts. This protoplast isolation and transient expression system is the most efficient based on the documented results to date. It can be widely used for cellular and molecular studies in orchids and other economically important monocot crops, especially for those lacking an efficient genetic transformation system in vivo.
Nitrogen and Stem Development: A Puzzle Still to Be Solved
Authors: Souza Lucas Anjos , Tavares Rafael
Published: February 2021

High crop yields are generally associated with high nitrogen (N) fertilizer rates. A growing tendency that is urgently demanding the adoption of precision technologies that manage N more efficiently, combined with the advances of crop genetics to meet the needs of sustainable farm systems. Among the plant traits, stem architecture has been of paramount importance to enhance harvest index in the cereal crops. Nonetheless, the reduced stature also brought undesirable effect, such as poor N-uptake, which has led to the overuse of N fertilizer. Therefore, a better understanding of how N signals modulate the initial and late stages of stem development might uncover novel semi-dwarf alleles without pleiotropic effects. Our attempt here is to review the most recent advances on this topic.
Early Warning Method for Public Health Emergency Under Artificial Neural Network in the Context of Deep Learning
Authors: Zheng Shuang , Hu Xiaomei
Published: February 2021

The purpose is to minimize the substantial losses caused by public health emergencies to people’s health and daily life and the national economy. The tuberculosis data from June 2017 to 2019 in a city are collected. The Structural Equation Model (SEM) is constructed to determine the relationship between hidden and explicit variables by determining the relevant indicators and parameter estimation. The prediction model based on Artificial Neural Network (ANN) and Convolutional Neural Network (CNN) is constructed. The method’s effectiveness is verified by comparing the prediction model’s loss value and accuracy in training and testing. Meanwhile, 50 pieces of actual cases are tested, and the warning level is determined according to the T-value. The results show that comparing and analyzing ANN, CNN, and the hybrid network of ANN and CNN, the hybrid network’s accuracy (95.1%) is higher than the other two algorithms, 89.1 and 90.1%. Also, the hybrid network has sound prediction effects and accuracy when predicting actual cases. Therefore, the early warning method based on ANN in deep learning has better performance in public health emergencies’ early warning, which is significant for improving early warning capabilities.
When Our Work Hits Home: Trauma and Mental Disorders in Correctional Officers and Other Correctional Workers
Authors: Fusco Nina , Ricciardelli Rosemary , Jamshidi Laleh , Carleton R. Nicholas , Barnim Nigel , Hilton Zoe , Groll Dianne
Published: February 2021

Background: International estimates suggest that up to one in three public safety personnel experience one or more mental disorders, including post-traumatic stress disorder (PTSD). Canadian data have been sparse until very recently, and correctional officers and forensic psychiatric staff have rarely been included. Working as a correctional officer is associated with negative health outcomes and increased work-related stress, with several variables affecting reported levels of stress. Healthcare staff also report higher rates of PTSD, especially those who are exposed to aggression in their workplace. In the present study, we compare current symptoms of diverse staff working in correctional occupations.

Method: Data were collected from a Canadian national online survey of public safety personnel, including employees of correctional services at the federal level. Correctional officers and wellness services staff were compared for prevalence of mental disorders and suicidal ideation.

Results: Correctional officers self-reported statistically significantly more exposure to potentially psychologically traumatic events than wellness services employees. Correctional officers also self-reported higher rates of symptoms of mental disorders, including PTSD, social anxiety, panic disorder, and depression. There were no statistically significant differences in reports of suicidal thoughts, plans, or attempts.

Contribution to Society: Correctional and forensic staff contribute to society by working with justice-involved individuals in correctional institutions. Trauma-related disorders and other mental health problems threaten the well-being of correctional and forensic staff. Mental health likely impacts the ability of correctional and forensic staff to develop a therapeutic or working alliance with persons in custody. Staff well-being must be recognized and addressed to ensure that prisoners and staff receive optimal treatment in prison.

Conclusion: Our results add to the limited knowledge about the well-being of staff, particularly wellness staff in prisons, who provide daily treatment and care for prisoners with serious mental disorders. Our work is a step toward identifying avenues for promoting staff well-being.
Creating Clean Air Spaces During Wildland Fire Smoke Episodes: Web Summit Summary
Authors: Davison Gilliane , Barkjohn Karoline K. , Hagler Gayle S. W. , Holder Amara L. , Coefield Sarah , Noonan Curtis , Hassett-Sipple Beth
Published: February 2021

Effective strategies to reduce indoor air pollutant concentrations during wildfire smoke events are critically needed. Worldwide, communities in areas prone to wildfires may suffer from annual smoke exposure events lasting from days to weeks. In addition, there are many areas of the world where high pollution events are common and where methods employed to reduce exposure to pollution may have relevance to wildfire smoke pollution episodes and vice versa. This article summarizes a recent virtual meeting held by the United States Environmental Protection Agency (EPA) to share research, experiences, and other information that can inform best practices for creating clean air spaces during wildland fire smoke events. The meeting included presentations on the public health impacts of wildland fire smoke; public health agencies' experiences and resilience efforts; and methods to improve indoor air quality, including the effectiveness of air filtration methods [e.g., building heating ventilation and air conditioning (HVAC) systems and portable, free-standing air filtration systems]. These presentations and related research indicate that filtration has been demonstrated to effectively improve indoor air quality during high ambient air pollution events; however, several research questions remain regarding the longevity and maintenance of filtration equipment during and after smoke events, effects on the pollution mixture, and degree to which adverse health effects are reduced.
A Multistage Process Model of How a Person Who Currently Injects Drugs Comes to Assist Persons Who Do not Inject with Their First Injections
Authors: Des Jarlais Don C. , Arasteh Kamyar , Barnes David M. , Feelemyer Jonathan , Berg Hayley , Raag Mait , Talu Ave , Org Greete , Tross Susan , Uuskula Anneli
Published: February 2021

Injecting drugs for the first time almost always requires assistance from an experienced person who injects drugs (PWID). While there has been moderate amount of research on PWID who assist with first injections, most of this research has focused on identifying characteristics of PWID who assist with first injections. We do not have a formal model that describes how the minority of PWID come to assist do so, while the majority never assist. Through comparison of persons who did or did not recently assist with first injections using data from PWID in Tallinn, Estonia (N = 286) and Staten Island, New York City (N = 101), we developed a formal multi-stage model of how PWID come to assist with first injections. The model had a primary pathway 1) of engaging in “injection promoting” behaviors, 2) being asked to assist, and 3) assisting. Statistical testing using odds ratios showed participation in each stage was strongly associated with participation in the next stage (all odds ratios >3.0) and the probabilities of assisting significantly increased with participation in the successive stages. We then used the model to compare engagement in the stages pre-vs. post participation in an intervention, and to compare persons who recently assisted to persons who had assisted in the past but had not recently assisted and to persons who had never assisted. Advantages of a formal model for how current PWID come to assist with first injections include: facilitating comparisons across different PWID populations and assessing strengths and limitations of interventions to reduce assisting with first injections.
Comparison of Changes in the Plumage and Body Condition, Egg Production, and Mortality of Different Non-Beak-Trimmed Pure Line Laying Hens during the Egg-Laying Period
Authors: Milisits Gábor , Szász Sándor , Donkó Tamás , Budai Zoltán , Almási Anita , Pőcze Olga , Ujvári Jolán , Farkas Tamás Péter , Garamvölgyi Erik , Horn Péter , Sütő Zoltán
Published: February 2021

The experiment was carried out with altogether 1740 non-beak-trimmed laying hens, which originated from Bábolna TETRA Ltd., representing two different types (Rhode Island Red (RIR) and Rhode Island White (RIW)) and four different lines (Lines 1–2: RIR, Lines 3–4: RIW). The plumage and body condition of randomly selected 120 hens (30 hens/line) was examined at 20, 46, and 62 weeks of age. The egg production and the mortality of the sampled hens were recorded daily. Based on the results, it was established that the lines differ clearly in most of the examined traits. It was also pointed out that injurious pecking of the hens resulted not only in damages in the plumage but also in the body condition. The results obviously demonstrated that the highest egg production and the lowest mortality rate were reached by those hens, which had the best plumage and body condition. Because the occurrence of injurious pecking seems to depend on the genetic background, selection of the hens (lines, families, individuals) for calm temperament will be very important in the future in order to maintain the high production level in non-beak-trimmed layer flocks.
Histone Deacetylase Inhibitors and Microtubule Inhibitors Induce Apoptosis in Feline Luminal Mammary Carcinoma Cells
Authors: Almeida Filipe , Gameiro Andreia , Correia Jorge , Ferreira Fernando
Published: February 2021

Feline mammary carcinoma (FMC) is the third most common type of neoplasia in cats, sharing similar epidemiological features with human breast cancer. In humans, histone deacetylases (HDACs) play an important role in the regulation of gene expression, with HDAC inhibitors (HDACis) disrupting gene expression and leading to cell death. In parallel, microtubules inhibitors (MTIs) interfere with the polymerization of microtubules, leading to cell cycle arrest and apoptosis. Although HDACis and MTIs are used in human cancer patients, in cats, data is scarce. In this study, we evaluated the antitumor properties of six HDACis (CI-994, panobinostat, SAHA, SBHA, scriptaid, and trichostatin A) and four MTIs (colchicine, nocodazole, paclitaxel, and vinblastine) using three FMC cell lines (CAT-MT, FMCp, and FMCm), and compared with the human breast cancer cell line (SK-BR-3). HDACis and MTIs exhibited dose-dependent antitumor effects in FMC cell lines, and for all inhibitors, the IC50 values were determined, with one feline cell line showing reduced susceptibility (FMCm). Immunoblot analysis confirmed an increase in the acetylation status of core histone protein HDAC3 and flow cytometry showed that HDACis and MTIs lead to cellular apoptosis. Overall, our study uncovers HDACis and MTIs as promising anti-cancer agents to treat FMCs.
Dietary Glutamic Acid Modulates Immune Responses and Gut Health of Weaned Pigs
Authors: Kyoung Hyunjin , Lee Jeong Jae , Cho Jin Ho , Choe Jeehwan , Kang Joowon , Lee Hanbae , Liu Yanhong , Kim Younghoon , Kim Hyeun Bum , Song Minho
Published: February 2021

Dietary glutamic acid (GLU) is used as a feed additive because of its functional characteristics that may affect the growth performance and health of pigs. This study was carried out to determine the effects of dietary GLU on growth performance, nutrient digestibility, immune responses, and intestinal health of weaned pigs. A total of ninety-six weaned pigs (8.07 ± 1.17 kg of body weight; 28 days of age) were assigned to two dietary treatments (8 pigs/pen; 6 replicates/treatment) in a randomized complete block design (block: body weight): (1) a typical weaner diet (CON) and (2) CON supplemented with 0.5% GLU. The experimental period was for 4 weeks. All data and sample collections were performed at the specific time points during the experimental period. Pigs fed GLU had higher average daily gain and average daily feed intake for the first two weeks and nutrient digestibility than pigs fed CON. In addition, dietary GLU increased villus height to crypt depth ratio, number of goblet cells, and ileal gene expression of claudin family and occludin compared with CON, but decreased serum TNF-α and IL-6 and ileal gene expression of TNF-α. Moreover, pigs fed GLU had increased relative composition of bacterial communities of genus Prevotella and Anaerovibrio and decreased genus Clostridium and Terrisporobacter compared with those fed CON. This study suggests that dietary GLU influences growth performance and health of weaned pigs by modulating nutrient digestibility, intestinal morphology, ileal gene expression of tight junction proteins and cytokines, immune responses, and microbial community in the gut.
The Extreme Function Theory for Damage Detection: An Application to Civil and Aerospace Structures
Authors: Martucci Davide , Civera Marco , Surace Cecilia
Published: February 2021

Any damaged condition is a rare occurrence for mechanical systems, as it is very unlikely to be observed. Thus, it represents an extreme deviation from the median of its probability distribution. It is, therefore, necessary to apply proper statistical solutions, i.e., Rare Event Modelling (REM). The classic tool for this aim is the Extreme Value Theory (EVT), which deals with uni- or multivariate scalar values. The Extreme Function Theory (EFT), on the other hand, is defined by enlarging the fundamental EVT concepts to whole functions. When combined with Gaussian Process Regression (GPR), the EFT is perfectly suited for mode shape-based outlier detection. In fact, it is possible to investigate the structure’s normal modes as a whole rather than focusing on their constituent data points, with quantifiable advantages. This provides a useful tool for Structural Health Monitoring, especially to reduce false alarms. This recently proposed methodology is here tested and validated both numerically and experimentally for different examples coming from Civil and Aerospace Engineering applications. One-dimensional beamlike elements with several boundary conditions are considered, as well as a two-dimensional plate-like spar and a frame structure.
Area and Volume of Remaining Cement and Enamel after Removal and Polishing of Buccal or Lingual Multibracket Appliances
Authors: Belanche Monterde Alba , Albaladejo Martínez Alberto , Curto Adrián , Alonso Pérez-Barquero Jorge , Guinot-Barona Clara , Zubizarreta-Macho Álvaro , Calama González Rosa María
Published: February 2021

The aim of the present study was to compare the area and volume of remaining cement after lingual and buccal multibracket appliance debonding. Further, the area and volume of cement remaining and the area and volume of enamel were also analyzed using a morphometric digital measurement technique. Ten buccal and 10 lingual multibracket appliances were cemented in 20 extracted teeth embedded into an epoxy resin model simulating a dental arch. The models were scanned before bonding the lingual and buccal multibracket appliances, after debonding the lingual and buccal multibracket appliances, and after polishing the remaining cement. Afterwards, the standard tessellation language (STL) digital files were aligned, segmented, and realigned by using engineer morphometry software. A comparative analysis was performed using Student’s t test statistical analysis. Lingual appliances showed statistically significantly (p < 0.001) less area (7.07 ± 4.85 mm2) and volume (0.87 ± 1.34 mm3) of remaining cement than the area (21.99 ± 4.18 mm2) and volume (p = 0.002) (3.48 ± 0.96 mm3) of buccal appliances. Moreover, lingual appliances showed statistically significantly (p = 0.001) less area (4.48 ± 3.08 mm2) and volume (0.13 ± 0.15 mm3) of remaining cement after polishing than the area (12.22 ± 5.98 mm2) and volume (p = 0.004) (0.70 ± 0.56 mm3) of buccal appliances. Lingual multibracket appliance therapy leads to less area and volume of cement remaining after multibracket appliance debonding and less area and volume of cement remaining after cement polishing than buccal multibracket appliance therapy; however, the area and volume of enamel removed after cement polishing were similar between both lingual and buccal multibracket appliance therapies.
Subjective Preferences and Discomfort Ratings of Backrest and Seat Pan Adjustments at Various Speeds
Authors: Li Jingdong , Huang Yu
Published: February 2021

Power seats (i.e., electrically adjustable seats that can be designed to move in several ways) have become increasingly common in airplanes, vehicles, and offices. Many studies have investigated the effects of seat attitude parameters, for example, the inclined angles of a backrest, on discomfort during the adjustment process. However, few studies have considered discomfort under different speeds during the adjustment process. In this study, we investigated discomfort with three speeds (i.e., “fast”, “median”, and “slow” corresponding to three durations of 15, 20, and 25 s, respectively) and two adjustments of a power seat, i.e., incline angle adjustment of the backrest and fore-and-aft position adjustment of the seat pan. We also investigated the effects of different physiological parameters on subjects’ discomfort. Twenty-four subjects (12 males and 12 females) completed a questionnaire to indicate their adjustment condition preferences, to rate their overall discomfort during the adjustment processes on a category-ratio scale, and to rate their local body discomfort. The majority of subjects preferred the fast speed adjustment condition and the trend was that a lower backrest adjustment speed increased discomfort during the process. The dominant local discomfort was in the upper and lower back regions during the backrest adjustment, whereas there was no obvious dominant local discomfort during the seat pan adjustment. The physiological parameters also had significant correlations with discomfort in some adjustment movements, for example, the discomfort was negatively correlated with height during the backrest adjustment.
A Synergistic Anti-Diabetic Effect by Ginsenosides Rb1 and Rg3 through Adipogenic and Insulin Signaling Pathways in 3T3-L1 Cells
Authors: Hong Hee-Do , Choi Sun-Il , Lee Ok-Hwan , Kim Young-Cheul
Published: February 2021

Although ginsenosides Rb1 and Rg3 have been identified as the significant ginsenosides found in red ginseng that confer anti-diabetic actions, it is unclear whether insulin-sensitizing effects are mediated by the individual compounds or by their combination. To determine the effect of ginsenosides Rb1 and Rg3 on adipocyte differentiation, 3T3-L1 preadipocytes were induced to differentiate the standard hormonal inducers in the absence or presence of ginsenosides Rb1 or Rg3. Additionally, we determined the effects of Rb1, Rg3, or their combination on the expression of genes related to adipocyte differentiation, adipogenic transcription factors, and the insulin signaling pathway in 3T3-L1 cells using semi-quantitative RT-PCR. Rb1 significantly increased the expression of CEBPα, PPARγ, and aP2 mRNAs. However, Rg3 exerted its maximal stimulatory effect on these genes at 1 μM concentration, while a high concentration (50 μM) showed inhibitory effects. Similarly, treatment with Rb1 and Rg3 (1 μM) increased the expression of IRS-1, Akt, PI3K, GLUT4, and adiponectin. Importantly, co-treatment of Rb1 and Rg3 (9:1) induced the maximal expression levels of these mRNAs. Our data indicate that the anti-diabetic activity of red ginseng is, in part, mediated by synergistic actions of Rb1 and Rg3, further supporting the significance of minor Rg3.
Probabilistic Forecasting of the 500 hPa Geopotential Height over the Northern Hemisphere Using TIGGE Multi-model Ensemble Forecasts
Authors: Ji Luying , Luo Qixiang , Ji Yan , Zhi Xiefei
Published: February 2021

Bayesian model averaging (BMA) and ensemble model output statistics (EMOS) were used to improve the prediction skill of the 500 hPa geopotential height field over the northern hemisphere with lead times of 1–7 days based on ensemble forecasts from the European Centre for Medium-Range Weather Forecasts (ECMWF), National Centers for Environmental Prediction (NCEP), and UK Met Office (UKMO) ensemble prediction systems. The performance of BMA and EMOS were compared with each other and with the raw ensembles and climatological forecasts from the perspective of both deterministic and probabilistic forecasting. The results show that the deterministic forecasts of the 500 hPa geopotential height distribution obtained from BMA and EMOS are more similar to the observed distribution than the raw ensembles, especially for the prediction of the western Pacific subtropical high. BMA and EMOS provide a better calibrated and sharper probability density function than the raw ensembles. They are also superior to the raw ensembles and climatological forecasts according to the Brier score and the Brier skill score. Comparisons between BMA and EMOS show that EMOS performs slightly better for lead times of 1–4 days, whereas BMA performs better for longer lead times. In general, BMA and EMOS both improve the prediction skill of the 500 hPa geopotential height field.
Circulating Cancer Biomarkers
Authors: Lokshin Anna , Bast Robert C. , Rodland Karin
Published: February 2021

Cancer is among the major public health problems worldwide, representing the leading cause of morbidity and mortality in industrialized countries [...]
Tumor Heterogeneity: A Great Barrier in the Age of Cancer Immunotherapy
Authors: El-Sayes Nader , Vito Alyssa , Mossman Karen
Published: February 2021

Throughout the history of oncology research, tumor heterogeneity has been a major hurdle for the successful treatment of cancer. As a result of aberrant changes in the tumor microenvironment such as high mutational burden, hypoxic conditions and abnormal vasculature, several malignant subpopulations often exist within a single tumor mass. Therapeutic intervention can also increase selective pressure towards subpopulations with acquired resistance. This phenomenon is often the cause of relapse in previously responsive patients, drastically changing the expected outcome of therapy. In the case of cancer immunotherapy, tumor heterogeneity is a substantial barrier as acquired resistance often takes the form of antigen escape and immunosuppression. In an effort to combat intrinsic resistance mechanisms, therapies are often combined as a multi-pronged approach to target multiple pathways simultaneously. These multi-therapy regimens have long been a mainstay of clinical oncology with chemotherapy cocktails but are more recently being investigated in the emerging landscape of immunotherapy. Furthermore, as high throughput technology becomes more affordable and accessible, researchers continue to deepen their understanding of the factors that influence tumor heterogeneity and shape the TME over the course of treatment regimens. In this review, we will investigate the factors that give rise to tumor heterogeneity and the impact it has on the field of immunotherapy. We will discuss how tumor heterogeneity causes resistance to various treatments and review the strategies currently being employed to overcome this challenging clinical hurdle. Finally, we will outline areas of research that should be prioritized to gain a better understanding of tumor heterogeneity and develop appropriate solutions.
The Florida Pancreas Collaborative Next-Generation Biobank: Infrastructure to Reduce Disparities and Improve Survival for a Diverse Cohort of Patients with Pancreatic Cancer
Authors: Permuth Jennifer , Dezsi Kaleena , Vyas Shraddha , Ali Karla , Basinski Toni , Utuama Ovie , Denbo Jason , Klapman Jason , Dam Aamir , Carballido Estrella , Kim Dae , Pimiento Jose , Powers Benjamin , Otto Amy , Choi Jung , Chen Dung-Tsa , Teer Jamie , Beato Francisca , Ward Alina , Cortizas Elena , Whisner Suzanne , Williams Iverson , Riner Andrea , Tardif Kenneth , Velanovich Vic , Karachristos Andreas , Douglas Wade , Legaspi Adrian , Allan Bassan , Meredith Kenneth , Molina-Vega Manual , Bao Philip , St. Julien Jamii , Huguet Kevin , Green Lee , Odedina Folakemi , Kumar Nagi , Simmons Vani , George Thomas , Vadaparampil Susan , Hodul Pamela , Arnoletti J. , Awad Ziad , Bose Debashish , Jiang Kun , Centeno Barbara , Gwede Clement , Malafa Mokenge , Judge Sarah , Judge Andrew , Jeong Daniel , Bloomston Mark , Merchant Nipun , Fleming Jason , Trevino Jose ,   on behalf of the Florida Pancreas Collaborative
Published: February 2021

Background: Well-annotated, high-quality biorepositories provide a valuable platform to support translational research. However, most biorepositories have poor representation of minority groups, limiting the ability to address health disparities. Methods: We describe the establishment of the Florida Pancreas Collaborative (FPC), the first state-wide prospective cohort study and biorepository designed to address the higher burden of pancreatic cancer (PaCa) in African Americans (AA) compared to Non-Hispanic Whites (NHW) and Hispanic/Latinx (H/L). We provide an overview of stakeholders; study eligibility and design; recruitment strategies; standard operating procedures to collect, process, store, and transfer biospecimens, medical images, and data; our cloud-based data management platform; and progress regarding recruitment and biobanking. Results: The FPC consists of multidisciplinary teams from fifteen Florida medical institutions. From March 2019 through August 2020, 350 patients were assessed for eligibility, 323 met inclusion/exclusion criteria, and 305 (94%) enrolled, including 228 NHW, 30 AA, and 47 H/L, with 94%, 100%, and 94% participation rates, respectively. A high percentage of participants have donated blood (87%), pancreatic tumor tissue (41%), computed tomography scans (76%), and questionnaires (62%). Conclusions: This biorepository addresses a critical gap in PaCa research and has potential to advance translational studies intended to minimize disparities and reduce PaCa-related morbidity and mortality.
Anti-Cancer Auto-Antibodies: Roles, Applications and Open Issues
Authors: de Jonge Hugo , Iamele Luisa , Maggi Maristella , Pessino Greta , Scotti Claudia
Published: February 2021

Auto-antibodies are classically associated with autoimmune diseases, where they are an integral part of diagnostic panels. However, recent evidence is accumulating on the presence of auto-antibodies against single or selected panels of auto-antigens in many types of cancer. Auto-antibodies might initially represent an epiphenomenon derived from the inflammatory environment induced by the tumor. However, their effect on tumor evolution can be crucial, as is discussed in this paper. It has been demonstrated that some of these auto-antibodies can be used for early detection and cancer staging, as well as for monitoring of cancer regression during treatment and follow up. Interestingly, certain auto-antibodies were found to promote cancer progression and metastasis, while others contribute to the body’s defense against it. Moreover, auto-antibodies are of a polyclonal nature, which means that often several antibodies are involved in the response to a single tumor antigen. Dissection of these antibody specificities is now possible, allowing their identification at the genetic, structural, and epitope levels. In this review, we report the evidence available on the presence of auto-antibodies in the main cancer types and discuss some of the open issues that still need to be addressed by the research community.
Effects of Alkali Metals on Nickel/Alumina Catalyzed Ethanol Dry Reforming
Authors: Park Se-Won , Lee Dongseok , Kim Seung-Ik , Kim Young Jin , Park Ji Hoon , Heo Iljeong , Chang Tae Sun , Lee Jin Hee
Published: February 2021

Although ethanol dry reforming is an attractive carbon utilization technology, problems of severe coke formation and low catalytic activity should be solved for realization of the technology. We demonstrate the effects of alkali metal additives (lithium, sodium, and potassium) on nickel catalyzed ethanol dry reforming. Potassium doped nickel catalyst (Ni/K2O-Al2O3) showed enhanced catalytic activity and durability in ethanol dry reforming. Thermogravimetric analysis (TGA) showed that Ni/K2O-Al2O3 had a high resistance to coke formation. The amounts of coke formed on Ni/K2O-Al2O3 were 1/3 lower than the amounts of coke formed on Ni/Al2O3. The total coke quantities were closely correlated to the number of basic sites of the nickel catalysts. Raman spectroscopy and transmission electron microscopy analyses revealed that the alkali metals control the coke formation on the catalysts.
Stereosopecificity in [Co(sep)][Co(edta)]Cl2·2H2O
Authors: Osvath Peter , Oliver Allen , Lappin A. Graham
Published: February 2021

The X-ray structure of racemic [Co(sep)][Co(edta)]Cl2·2H2O is reported and reveals hetero-chiral stereospecificity in the interactions of [Co(sep)]3+ with [Co(edta)]−. Hydrogen-bonding along the molecular C2-axes of both complexes accounts for the stereospecificity. The structure of Λ-[Co(en)3]∆-[Co(edta)]2Cl·10H2O has been re-determined. Previous structural data for this compound were collected at room temperature and the model did not sufficiently describe the disorder in the structure. The cryogenic temperature used in the present study allows the disorder to be conformationally locked and modeled more reliably. A clearer inspection of other, structurally interesting, interactions is possible. Again, hydrogen-bonding along the molecular C2-axis of [Co(en)3]3+ and the equatorial carboxylates of [Co(edta)]− is the important interaction. The unique nature of the equatorial carboxylates and molecular C2-axis in [Co(edta)]−, straddled by two pseudo-C3-faces where the arrangement of the carboxylate groups conveys the same helicity, is highlighted. Implications of these structures in understanding stereoselectivity in ion-pairing and electron transfer reactions are discussed.
Risk Factors for Bullying Victimization in Children with Neurofibromatosis Type 1 (NF1)
Authors: Stavinoha Peter L. , Solesbee Cody , Swearer Susan M. , Svoboda Steven , Klesse Laura J. , Holland Alice Ann
Published: February 2021

Neurofibromatosis type 1 (NF1) is an autosomal disorder associated with numerous physical stigmata. Children with NF1 are at known risk for attention-deficit/hyperactivity disorder (ADHD), academic struggles, and significant social difficulties and adverse social outcomes, including bullying victimization. The primary aim of this study was to identify risk factors associated with bullying victimization in children with NF1 to better inform clinicians regarding targets for prevention and clinical intervention. Children and a parent completed questionnaires assessing the bully victim status, and parents completed a measure of ADHD symptoms. Analyses were completed separately for parent-reported victimization of the child and the child’s self-report of victimization. According to the parent report, results suggest ADHD symptoms are a significant risk factor for these children being a target of bullying. Findings for academic disability were not conclusive, nor were findings related to having a parent with NF1. Findings indicate the need for further research into possible risk factors for social victimization in children with NF1. Results provide preliminary evidence that may guide clinicians working with children with NF1 and their parents in identifying higher-risk profiles that may warrant earlier and more intensive intervention to mitigate later risk for bullying victimization.
Effects of Variable Transport Properties on Heat and Mass Transfer in MHD Bioconvective Nanofluid Rheology with Gyrotactic Microorganisms: Numerical Approach
Authors: Awais Muhammad , Ehsan Awan Saeed , Asif Zahoor Raja Muhammad , Parveen Nabeela , Khan Wasim Ullah , Yousaf Malik Muhammad , He Yigang
Published: February 2021

Rheology of MHD bioconvective nanofluid containing motile microorganisms is inspected numerically in order to analyze heat and mass transfer characteristics. Bioconvection is implemented by combined effects of magnetic field and buoyancy force. Gyrotactic microorganisms enhance the heat and transfer as well as perk up the nanomaterials’ stability. Variable transport properties along with assisting and opposing flow situations are taken into account. The significant influences of thermophoresis and Brownian motion have also been taken by employing Buongiorno’s model of nanofluid. Lie group analysis approach is utilized in order to compute the absolute invariants for the system of differential equations, which are solved numerically using Adams-Bashforth technique. Validity of results is confirmed by performing error analysis. Graphical and numerical illustrations are prepared in order to get the physical insight of the considered analysis. It is observed that for controlling parameters corresponding to variable transport properties c2, c4, c6, and c8, the velocity, temperature, concentration, and bioconvection density distributions accelerates, respectively. While heat and mass transfer rates increases for convection parameter and bioconvection Rayleigh number, respectively.
Biodiversity of Mites
Authors: Gwiazdowicz Dariusz J.
Published: February 2021

Nature is a highly complex, intricate system constructed of a network of interdependencies between individual species, their communities and their habitats [...]