bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamic Evolution and Correlation between Metabolites and Microorganisms during Manufacturing Process and Storage of Fu Brick Tea
Authors: Li Jing , Xu Ran , Zong Lixuan , Brake Joseph , Cheng Lizeng , Wu Jie , Wu Xiaobin
Published: October 2021

Fu brick tea (FBT) is one of the major brands of dark tea. Microbial fermentation is considered the key step in the development of the special characteristics of FBT. The systemic corelationship of the microbiome and metabolomics during manufacture of Fu brick tea is not fully understood. In this study, we comprehensively explored the microbiome and metabolite dynamic evolution during the FBT manufacturing processes, and revealed decisive factors for the quality and safety of FBT based on the grouped methods of metabolomics combined with biochemical measurements, microbiome sequencing combined with quantitative polymerase chain reaction (PCR), and multiplex analysis. Both the microbiome and quantitative PCR showed that fungi displayed concentrated distribution characteristics in the primary dark tea samples, while bacterial richness increased during the flowering processes and ripening period. All microorganism species, as well as dominant fungi and bacteria, were identified in the distinct processes periods. A total of 178 metabolites were identified, and 34 of them were characterized as critical metabolites responsible for metabolic changes caused by the corresponding processes. Metabolic analysis showed that most metabolites were decreased during the FBT manufacturing processes, with the exception of gallic acid. Multivariate analysis verified that the critical metabolites were correlated with specific dominant microbial species. All the top fungal species except unclassified_g_ Aspergillus showed positive correlations with six critical metabolites (L-The, epigallocatechin (EGC), Gln, tea polyphenol (TP), tea polysaccharides (TPs) and caffeine). Five of the top bacteria species (Cronobacter, Klebsiella, Pantoea, Pluralibacter, and unclassified_ f_Entero-bacteriaceae) showed positive correlations with epigallocatechins and tea polyphenols, while the other 11 top bacterial species correlated negatively with all the critical metabolites. The content of amino acids, tea polyphenols, tea polysaccharides, and flavonoids was reduced during microbial fermentation. In conclusion, our results reveal that microbial composition is the critical factor in changing the metabolic profile of FBT. This discovery provides a theoretical basis for improving the quality of FBT and enhancing its safety.
Optical Fiber Sensor for Temperature and Strain Measurement Based on Multimode Interference and Square-Core Fiber
Authors: Wang Kun , Dong Xingchen , Kienle Patrick , Fink Maximilian , Kurz Wolfgang , Köhler Michael H. , Jakobi Martin , Koch Alexander W.
Published: October 2021

A variety of specialty fibers such as no-core fiber (NCF) have already been studied to reveal their sensing abilities. In this work, we investigate a specialty fiber, square-core fiber, for temperature and strain sensing. A simple single-mode–multimode–single-mode (SMS) fiber sensor was fabricated, consisting of a 30-cm-long square-core fiber. The experimental results indicate that the maximal wavelength-temperature and wavelength-strain sensitivities are −15.3 pm/∘C and −1.5 pm/με, respectively, while the maximal power-temperature and power-strain sensitivities are 0.0896 dBm/∘C and 0.0756 dBm/με. Analysis of the results suggests that the fiber sensor has the potential to be used as a high-sensitivity temperature sensor with a low strain sensitivity.
The Effect of the Layered Internal Structure of Fibrous Beds on the Hydrodynamic Diffusive Behavior of Microparticles
Authors: Otomo Ryoko , Kira Ryosuke
Published: October 2021

To separate and collect microparticles such as cells, the behavior of particles in fibrous filters was investigated. It is essential to understand, in detail, the motion of particles in microscale flows, because Re is often small, and particles exhibit complex behaviors such as changes in relative position and spreading owing to hydrodynamic interactions. We calculated the motion of microparticles passing through the fibrous bed using the Stokesian dynamics method, in which hydrodynamic interaction is considered, theoretically. The fibrous bed was modeled by particles and five types of structures (a monolayer with fiber volume fractions φ of 3%, 4%, and 5%, and a bilayer with φ = 3%−5% and 5%−3%) were considered. Our numerical results showed that the particles moved in a complicated manner, and spread throughout the fibrous bed. It was found that the behavior of individual microparticles varied depending on the internal structure, although the average permeation velocity was primarily determined by the fiber volume fraction. This great dependence of the behavior of particle assemblage on the internal structure of the fibrous bed was caused by the individual particle motion under the influence of the layers in front of and behind them, owing to the hydrodynamic interaction.
Concealed-Fault Detection in Low-Amplitude Tectonic Area—An Example of Tight Sandstone Reservoirs
Authors: Wang Enli , Zhang Junduo , Yan Guoliang , Yang Qing , Zhao Wanjin , Xie Chunhui , He Run
Published: October 2021

Fault detection is important to seismic interpretation, especially for tight oil and gas reservoirs. Generally speaking, large-scale faults can be accurately imaged and are easy to detect by conventional methods, but the concealed ones in low-amplitude structural regions are difficult to find. In these areas, the scale and displacement of concealed faults are usually very small. Due to the good uniform and weak amplitude disturbances in the seismic events, the traditional discontinuity attributes extracted from seismic data are always not effective. This is because the discontinuous features of large faults are very significant, and the weak anomalies caused by hidden faults are very close to the continuous background. This paper takes a tight sandstone reservoir in the Ordos Basin of China as an example to explore the detection method of subtle faults in low-amplitude structural areas. With the phase congruency analysis method, we extract edge features from the post-stack coherence attributes to identify hidden faults. Practice shows that this idea has outstanding performance in mining hidden fracture features and improving the accuracy of fracture recognition. The results successfully predict a shear fault zone in the northeast of the work area, find a new fracture zone in the center of the survey and a series of hidden faults in non-target strata. It would be beneficial to extend the strata and area of oil and gas reservoirs.
Coupled Thermodynamics and Phase Diagram Analysis of Gas-Duct Concretion Formation in Pyro-Processing Ironmaking and Steelmaking Dust
Authors: Wang Daya , Hua Shaoguang , Wu Liushun , Liu Kunlong , Wang Haichuan
Published: October 2021

In recent years, the steel industry has accumulated approximately 100 million tons of dust annually, severely threatening the environment. Rotary kiln technology is one of the main industrial methods used to process this dust. However, some substances in flue gas congeal on the cooling wall of the gas duct and seriously affect production. In this study, the properties and formation mechanisms of the coagulum were investigated on the basis of experimental and thermodynamic analyses. The experimental results showed that the coagulum is mainly composed of chlorides (KCl, NaCl, and ZnCl2), oxides (ZnO, FeO), and carbon, with three structures: lumps, fibers, and particles. Based on a thermodynamic analysis, a reasonable explanation was proposed to clarify the formation mechanism. The liquid phase (a eutectic system of KCl–NaCl–ZnCl2), dendrites (KCl, NaCl), and particles (ZnO, FeO, C) were found to act as binders, stiffeners, and aggregates in the coagulum, respectively, constituting a composite structure. Liquids acting as binders are essential for coagulum formation, and dendrites and particles strengthen this effect. Furthermore, the eutectic system of chlorides plays a crucial role in coagulum formation. The results of the present study offer a theoretical understanding of gas-duct coagulation and will provide guidance for adopting alleviation measures.
Fundamental Methods for the Phase Transfer of Nanoparticles
Authors: Cook Elijah , Labiento Gianna , Chauhan Bhanu P. S.
Published: October 2021

The utilization of nanoparticles for a variety of applications has raised much interest in recent years as new knowledge has emerged in nanochemistry. New and diverse methods for synthesis, characterization, and application of these particles have been discovered with differing degrees of ease and reproducibility. Post-synthetic modification of nanoparticles is often a required step to facilitate their use in applications. The reaction conditions and chemical environment for the nanoparticle synthesis may not support or may conflict with further reactions. For this reason, it is beneficial to have phase transfer methods for nanoparticles to allow for their dispersion in a variety of solvents. Phase transfer methods are often limited in the types and sizes of particles that can be effectively dispersed in an immiscible solvent. Currently, general transfer methods for a wide variety of nanoparticles have not been identified. New routes for phase transfer allow for utilization of a larger range of particles in applications which were previously limited by solubility and reactivity issues. In this work, we will describe the fundamental methods for the phase transfer of metallic nanoparticles. We will look at the major problems and pitfalls of these methods. The applications of phase transfer will also be reviewed, mainly focusing on catalysis and drug delivery.
Geographical Discrimination of Bell Pepper (Capsicum annuum) Spices by (HS)-SPME/GC-MS Aroma Profiling and Chemometrics
Authors: Reale Samantha , Biancolillo Alessandra , Gasparrini Chiara , Di Martino Luciano , Di Cecco Valter , Manzi Aurelio , Di Santo Marco , D’Archivio Angelo Antonio
Published: October 2021

Dried and ground red pepper is a spice used as seasoning in various traditional dishes all over the world; nevertheless, the pedoclimatic conditions of the diverse cultivation areas provide different chemical characteristics, and, consequently, diverse organoleptic properties to this product. In the present study, the volatile profiles of 96 samples of two different ground bell peppers harvested in diverse Italian geographical areas, Altino (Abruzzo) and Senise (Lucania), and a commercial sweet paprika, have been studied by means of headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). The investigation of their volatile profile has led to the identification of 59 analytes. Eventually, a discriminant classifier, Partial Least Squares Discriminant Analysis (PLS-DA), was exploited to discriminate samples according to their geographical origin. The model provided very accurate results in external validation; in fact, it correctly classified all the 30 test samples, achieving 100% correct classification (on the validation set). Furthermore, in order to understand which volatiles contribute the most at differentiating the bell peppers from the different origins, a variable selection approach, Variable Importance in Projection (VIP), was used. This strategy led to the selection of sixteen diverse compounds which characterize the different bell pepper spices.
In Vivo Antimalarial Activity of Leaf Extracts and a Major Compound Isolated from Ranunculus multifidus Forsk
Authors: Sirak Betelhem , Mann Lea , Richter Adrian , Asres Kaleab , Imming Peter
Published: October 2021

The leaves of Ranunculus multifidus Forsk. are traditionally used for the treatment of malaria in several African countries. In the present study, 80% methanol (RM-M) and hydrodistilled (RM-H) extracts of fresh leaves from R. multifidus and its major constituent anemonin were tested for their in vivo antimalarial activity against Plasmodium berghei in mice. Anemonin was also tested for its in vitro antimycobacterial activity against Mycobacterium smegmatis and M. abscessus in a microbroth dilution assay, and bacterial growth was analyzed by OD measurement. The isolation of anemonin from RM-H was carried out using preparative thin layer chromatography (PTLC). The chemical structures of anemonin and its hydrolysis product were elucidated using spectroscopic methods (HR–MS; 1D and 2D-NMR). Results of the study revealed that both RM-M and RM-H were active against P. berghei in mice, although the latter demonstrated superior activity (p < 0.001), as compared to the former. At a dose of 35.00 mg/kg/day, RM-H demonstrated a chemosuppression value of 70% in a 4-day suppressive test. In a 4-day suppressive, Rane’s and prophylactic antimalarial tests, anemonin showed median effective doses (ED50s) of 2.17, 2.78 and 2.70 μM, respectively. However, anemonin did not inhibit the growth of M. smegmatis and M. abscessus.
Identification of Antiprotozoal Compounds from Buxus sempervirens L. by PLS-Prediction
Authors: Szabó Lara U. , Kaiser Marcel , Mäser Pascal , Schmidt Thomas J.
Published: October 2021

Various nor-triterpene alkaloids of Buxus (B.) sempervirens L. have shown remarkable in vitro activity against the causative agents of tropical malaria and East African sleeping sickness. To identify further antiprotozoal compounds of this plant, 20 different fractions of B. sempervirens L., exhibiting a wide range of in vitro bioactivity, were analyzed by UHPLC/+ESI-QqTOF-MS/MS. The analytical profiles were investigated by partial least squares regression (PLS) for correlations between the intensity of LC/MS signals, bioactivity and cytotoxicity. The resulting models highlighted several compounds as mainly responsible for the antiprotozoal activity and thus, worthwhile for subsequent isolation. These compounds were dereplicated based on their mass spectra in comparison with isolated compounds recently reported by us and with literature data. Moreover, an estimation of the cytotoxicity of the highlighted compounds was derived from an additional PLS model in order to identify plant constituents with strong selectivity. In conclusion, high levels of antitrypanosomal and antiplasmodial activity were predicted for eight and four compounds, respectively. These include three hitherto unknown constituents of B. sempervirens L., presumably new natural products.
Using Shallow and Deep Learning to Automatically Detect Hate Motivated by Gender and Sexual Orientation on Twitter in Spanish
Authors: Arcila-Calderón Carlos , Amores Javier J. , Sánchez-Holgado Patricia , Blanco-Herrero David
Published: October 2021

The increasing phenomenon of “cyberhate” is concerning because of the potential social implications of this form of verbal violence, which is aimed at already-stigmatized social groups. According to information collected by the Ministry of the Interior of Spain, the category of sexual orientation and gender identity is subject to the third-highest number of registered hate crimes, ranking behind racism/xenophobia and ideology. However, most of the existing computational approaches to online hate detection simultaneously attempt to address all types of discrimination, leading to weaker prototype performances. These approaches focus on other reasons for hate—primarily racism and xenophobia—and usually focus on English messages. Furthermore, few detection models have used manually generated databases as a training corpus. Using supervised machine learning techniques, the present research sought to overcome these limitations by developing and evaluating an automatic detector of hate speech motivated by gender and sexual orientation. The focus was Spanish-language posts on Twitter. For this purpose, eight predictive models were developed from an ad hoc generated training corpus, using shallow modeling and deep learning. The evaluation metrics showed that the deep learning algorithm performed significantly better than the shallow modeling algorithms, and logistic regression yielded the best performance of the shallow algorithms.
An Overview of Olfactory Displays in Education and Training
Authors: Garcia-Ruiz Miguel Angel , Kapralos Bill , Rebolledo-Mendez Genaro
Published: October 2021

This paper describes an overview of olfactory displays (human–computer interfaces that generate and diffuse an odor to a user to stimulate their sense of smell) that have been proposed and researched for supporting education and training. Past research has shown that olfaction (the sense of smell) can support memorization of information, stimulate information recall, and help immerse learners and trainees into educational virtual environments, as well as complement and/or supplement other human sensory channels for learning. This paper begins with an introduction to olfaction and olfactory displays, and a review of techniques for storing, generating and diffusing odors at the computer interface. The paper proceeds with a discussion on educational theories that support olfactory displays for education and training, and a literature review on olfactory displays that support learning and training. Finally, the paper summarizes the advantages and challenges regarding the development and application of olfactory displays for education and training.
Reduced Graphene-Oxide-Encapsulated MoS2/Carbon Nanofiber Composite Electrode for High-Performance Na-Ion Batteries
Authors: Cho Su-Ho , Kim Jong-Heon , Kim Il-Gyu , Park Jeong-Ho , Jung Ji-Won , Kim Hyun-Suk , Kim Il-Doo
Published: October 2021

Sodium-ion batteries (SIBs) have been increasingly studied due to sodium (Na) being an inexpensive ionic resource (Na) and their battery chemistry being similar to that of current lithium-ion batteries (LIBs). However, SIBs have faced substantial challenges in developing high-performance anode materials that can reversibly store Na+ in the host structure. To address these challenges, molybdenum sulfide (MoS2)-based active materials have been considered as promising anodes, owing to the two-dimensional layered structure of MoS2 for stably (de)inserting Na+. Nevertheless, intrinsic issues of MoS2—such as low electronic conductivity and the loss of active S elements after a conversion reaction—have limited the viability of MoS2 in practical SIBs. Here, we report MoS2 embedded in carbon nanofibers encapsulated with a reduced graphene oxide (MoS2@CNFs@rGO) composite for SIB anodes. The MoS2@CNFs@rGO delivered a high capacity of 345.8 mAh g−1 at a current density of 100 mA g−1 for 90 cycles. The CNFs and rGO were synergistically taken into account for providing rapid pathways for electrons and preventing the dissolution of S sources during repetitive conversion reactions. This work offers a new point of view to realize MoS2-based anode materials in practical SIBs.
Strain Effects on the Electronic and Optical Properties of Kesterite Cu2ZnGeX4 (X = S, Se): First-Principles Study
Authors: El Hamdaoui Jawad , El-Yadri Mohamed , Farkous Mohamed , Kria Mohamed , Courel Maykel , Ojeda Miguel , Pérez Laura M. , Tiutiunnyk Anton , Laroze David , Feddi El Mustapha
Published: October 2021

Following the chronological stages of calculations imposed by the WIEN2K code, we have performed a series of density functional theory calculations, from which we were able to study the effect of strain on the kesterite structures for two quaternary semiconductor compounds Cu2ZnGeS4 and Cu2ZnGeSe4. Remarkable changes were found in the electronic and optical properties of these two materials during the application of biaxial strain. Indeed, the band gap energy of both materials decreases from the equilibrium state, and the applied strain is more pronounced. The main optical features are also related to the applied strain. Notably, we found that the energies of the peaks present in the dielectric function spectra are slightly shifted towards low energies with strain, leading to significant refraction and extinction index responses. The obtained results can be used to reinforce the candidature of Cu2ZnGeX4(X = S, Se) in the field of photovoltaic devices.
Visible-Light-Assisted Photoelectrochemical Biosensing of Uric Acid Using Metal-Free Graphene Oxide Nanoribbons
Authors: Sun Chia-Liang , Lin Cheng-Hsuan , Kuo Chia-Heng , Huang Chia-Wei , Nguyen Duc Dung , Chou Tsu-Chin , Chen Cheng-Ying , Lu Yu-Jen
Published: October 2021

In this study, we demonstrate the visible-light-assisted photoelectrochemical (PEC) biosensing of uric acid (UA) by using graphene oxide nanoribbons (GONRs) as PEC electrode materials. Specifically, GONRs with controlled properties were synthesized by the microwave-assisted exfoliation of multi-walled carbon nanotubes. For the detection of UA, GONRs were adopted to modify either a screen-printed carbon electrode (SPCE) or a glassy carbon electrode (GCE). Cyclic voltammetry analyses indicated that all Faradaic currents of UA oxidation on GONRs with different unzipping/exfoliating levels on SPCE increased by more than 20.0% under AM 1.5 irradiation. Among these, the GONRs synthesized under a microwave power of 200 W, namely GONR(200 W), exhibited the highest increase in Faradaic current. Notably, the GONR(200 W)/GCE electrodes revealed a remarkable elevation (~40.0%) of the Faradaic current when irradiated by light-emitting diode (LED) light sources under an intensity of illumination of 80 mW/cm2. Therefore, it is believed that our GONRs hold great potential for developing a novel platform for PEC biosensing.
Efficient Removal of Hexavalent Chromium from an Aquatic System Using Nanoscale Zero-Valent Iron Supported by Ramie Biochar
Authors: Tan Xiangpeng , Shaaban Muhammad , Yang Jianwei , Cai Yajun , Wang Buyun , Peng Qi-An
Published: October 2021

In this study, ramie biochar (RBC) was used to activate nano zero-valent iron (nZVI) to enhance hexavalent chromium (Cr(VI)) removal. The best results were obtained at a pyrolysis temperature of 600 °C, a biochar particle size of < 150 μm, and an iron to carbon ratio = 1:1. Under the optimal conditions, the removal of Cr(VI) by RBC600-nZVI (98.69%) was much greater than that of RBC600 (12.42%) and nZVI (58.26%). Scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS) revealed that the reaction mechanism at the Fe and Cr interface was a multiple interaction mechanism with reduction dominated, adsorption, and co-precipitation simultaneously. The enhanced performance of RBC600-nZVI resulted from the effective dispersion of nZVI on the surface of RBC600, therefore increasing the adsorption activity sites. At the same time, RBC600 and nZVI exerted a synergistic influence on the composite structure, which jointly promoted the reduction reaction of Cr(VI) and removed more Cr(VI). This study shows that RBC-nZVI is a potentially valuable remediation material that not only provides a new idea for the utilization of ramie waste, but also effectively overcomes the limitations of nZVI, thus, achieving efficient and rapid remediation of Cr(VI).
Advances in Nanomaterials-Based Electrochemical Biosensors for Foodborne Pathogen Detection
Authors: Bobrinetskiy Ivan , Radovic Marko , Rizzotto Francesco , Vizzini Priya , Jaric Stefan , Pavlovic Zoran , Radonic Vasa , Nikolic Maria Vesna , Vidic Jasmina
Published: October 2021

Electrochemical biosensors utilizing nanomaterials have received widespread attention in pathogen detection and monitoring. Here, the potential of different nanomaterials and electrochemical technologies is reviewed for the development of novel diagnostic devices for the detection of foodborne pathogens and their biomarkers. The overview covers basic electrochemical methods and means for electrode functionalization, utilization of nanomaterials that include quantum dots, gold, silver and magnetic nanoparticles, carbon nanomaterials (carbon and graphene quantum dots, carbon nanotubes, graphene and reduced graphene oxide, graphene nanoplatelets, laser-induced graphene), metal oxides (nanoparticles, 2D and 3D nanostructures) and other 2D nanomaterials. Moreover, the current and future landscape of synergic effects of nanocomposites combining different nanomaterials is provided to illustrate how the limitations of traditional technologies can be overcome to design rapid, ultrasensitive, specific and affordable biosensors.
Modification of Aerosol Gold Nanoparticles by Nanosecond Pulsed-Periodic Laser Radiation
Authors: Khabarov Kirill , Nouraldeen Messan , Tikhonov Sergei , Lizunova Anna , Efimov Alexey , Ivanov Victor
Published: October 2021

This study investigates the processes of interaction of nanosecond pulsed-periodic laser radiation with the flow of aerosol agglomerates of gold nanoparticles synthesized in a spark discharge. Nanoparticles in a gas flow are spatially separated nano-objects whose interaction with each other and with the walls of an experimental cell was insignificant. Therefore, the energy absorbed by nanoparticles was used only for their own heating with further shape and size modification and on heat transfer to the surrounding gas. In the research, we used laser radiation with wavelengths of 527 and 1053 nm at pulse energies up to 900 µJ and pulse repetition rates up to 500 Hz. The dynamics of changes in the nanoparticles size during their sintering process depending on the laser pulses energy is characterized by an S-shaped shrinkage curve. Complete sintering of the initial agglomerates with their transformation into spherical nanoparticles is achieved by a series of impacting laser pulses. The result of nanoparticles’ laser modification is largely determined by the pulse energy and the efficiency of the nanoparticles’ radiation absorption.
Synthesis of a Novel Series of Cu(I) Complexes Bearing Alkylated 1,3,5-Triaza-7-phosphaadamantane as Homogeneous and Carbon-Supported Catalysts for the Synthesis of 1- and 2-Substituted-1,2,3-triazoles
Authors: Librando Ivy L. , Mahmoud Abdallah G. , Carabineiro Sónia A. C. , Guedes da Silva M. Fátima C. , Geraldes Carlos F. G. C. , Pombeiro Armando J. L.
Published: October 2021

The N-alkylation of 1,3,5-triaza-7-phosphaadamantane (PTA) with ortho-, meta- and para-substituted nitrobenzyl bromide under mild conditions afforded three hydrophilic PTA ammonium salts, which were used to obtain a new set of seven water-soluble copper(I) complexes. The new compounds were fully characterized and their catalytic activity was investigated for the low power microwave assisted one-pot azide–alkyne cycloaddition reaction in homogeneous aqueous medium to obtain disubstituted 1,2,3-triazoles. The most active catalysts were immobilized on activated carbon (AC), multi-walled carbon nanotubes (CNT), as well as surface functionalized AC and CNT, with the most efficient support being the CNT treated with nitric acid and NaOH. In the presence of the immobilized catalyst, several 1,4-disubstituted-1,2,3-triazoles were obtained from the reaction of terminal alkynes, organic halides and sodium azide in moderate yields up to 80%. Furthermore, the catalyzed reaction of terminal alkynes, formaldehyde and sodium azide afforded 2-hydroxymethyl-2H-1,2,3-triazoles in high yields up to 99%. The immobilized catalyst can be recovered and recycled through simple workup steps and reused up to five consecutive cycles without a marked loss in activity. The described catalytic systems proceed with a broad substrate scope, under microwave irradiation in aqueous medium and according to “click rules”.
Time-Restricted Feeding during Puberty Ameliorates Adiposity and Prevents Hepatic Steatosis in a Mouse Model of Childhood Obesity
Authors: Ribas-Aulinas Francesc , Parra-Vargas Marcela , Ramon-Krauel Marta , Diaz Ruben , Lerin Carles , Cambras Trinitat , Jimenez-Chillaron Josep C.
Published: October 2021

Background: Time restricted feeding (TRF) refers to dietary interventions in which food access is limited during a specific timeframe of the day. TRFs have proven useful in improving metabolic health in adult subjects with obesity. Their beneficial effects are mediated, in part, through modulating the circadian rhythm. Nevertheless, the translation of these dietary interventions onto obese/overweight children and adolescents remains uncharacterized. The objective of this study is to explore the feasibility of temporal dietary interventions for improving metabolic health in the context of childhood obesity. Methods: We have previously developed a mouse model of early adiposity (i.e., childhood obesity) through litter size reduction. Mice raised in small litters (SL) became obese as early as by two weeks of age, and as adults, they developed several obesity-related co-morbidities, including insulin resistance, glucose intolerance and hepatic steatosis. Here, we explored whether two independent short-term chrono-nutritional interventions might improve metabolic health in 1-month-old pre-pubertal SL mice. Both TRFs comprised 8 h feeding/14 h fasting. In the first one (TRF1) Control and SL mice had access to the diet for 8 h during the dark phase. In the second intervention (TRF2) food was available during the light:dark transitions. Results: TRF1 did not alter food intake nor ameliorate adiposity in SL-TRF1. In contrast, SL-TRF2 mice showed unintentional reduction of caloric intake, which was accompanied by reduced total body weight and adiposity. Strikingly, hepatic triglyceride content was completely normalized in SL-TRF1 and SL-TRF2 mice, when compared to the ad lib-fed SL mice. These effects were partially mediated by (i) clock-dependent signals, which might modulate the expression of Pparg or Cpt1a, and (ii) clock-independent signals, such as fasting itself, which could influence Fasn expression. Conclusions: Time-restricted feeding is an effective and feasible nutritional intervention to improve metabolic health, namely hepatic steatosis, in a model of childhood obesity. These data open new avenues for future safe and efficient chrono-nutritional interventions aimed to improve metabolic health in children with overweight/obesity.
Higher Habitual Nuts Consumption Is Associated with Better Cognitive Function among Qatari Adults
Authors: Nafea Hajer , Abdelmegid Omnia , Qaddourah Sara , Abdulwahab Zainab , Moawad Joyce , Shi Zumin
Published: October 2021

The association between nuts intake and cognitive function is inconclusive. We aimed to investigate the association between habitual nuts consumption and cognition among Qatari adults. Data from 1000 participants aged >20 years who attended Qatar Biobank (QBB) were used. Nuts consumption was assessed by a food frequency questionnaire (FFQ). Blood samples were measured for magnesium, lipids and glucose. Mean reaction time (MRT) was used as an indicator of cognitive function. Linear regression was used to assess the association. A total of 21.1% of the participants reported consuming nuts ≥4–6 times/week (high consumption) while 40.2% reported consuming ≤1 time/month (low consumption). The mean MRT was 715.6 milliseconds (SD 204.1). An inverse association was found between nuts consumption and MRT. Compared to those with a low consumption, high consumption of nuts had a regression coefficient of −36.9 (95% CI −68.1 to −5.8) after adjusting for sociodemographic and lifestyle factors. The inverse association between nuts and MRT was mainly seen among those >50 years. There was an interaction between nuts consumption and hypertension. The association between nuts consumption and MRT was not mediated by hypertension, diabetes, or serum magnesium. Habitual higher consumption of nuts is positively associated with cognitive function, especially among old adults.
The Association between Malnutrition and Oral Health in Older People: A Systematic Review
Authors: Algra Yne , Haverkort Elizabeth , Kok Wilhelmina , Etten-Jamaludin Faridi van , Schoot Liedeke van , Hollaar Vanessa , Naumann Elke , Schueren Marian de van der , Jerković-Ćosić Katarina
Published: October 2021

The aim of this systematic review was to examine the association between malnutrition and oral health in older people (≥ 60 years of age). A comprehensive systematic literature search was performed in four databases (PubMed, CINAHL, Dentistry and Oral Sciences Source, and Embase) for literature from January 2000 to May 2020. Both observational and intervention studies were screened for eligibility. Two reviewers independently screened the search results to identify potential eligible studies, and assessed the methodological quality of the full-text studies. A total of 3240 potential studies were identified. After judgement for relevance, 10 studies (cross-sectional (n = 9), prospective cohort (n = 1)) met the inclusion criteria. Three studies described malnourished participants as having fewer teeth, or functional (tooth) units (FTUs), compared to well-nourished participants. Four studies reported soft tissue problems in malnourished participants, including red tongue with blisters, and dry or cracked lips. Subjective oral health was the topic in six studies, with poorer oral health and negative self-perception of oral health in malnourished elderly participants. There are associations between (at risk of) malnutrition and oral health in older people, categorized in hard and soft tissue conditions of the mouth, and subjective oral health. Future research should be focused on longitudinal cohort studies with proper determination of malnutrition and oral health assessments, in order to evaluate the actual association between malnutrition and oral health in older people.
A Role for Peripheral Anandamide and 2-Arachidonoylglycerol in Short-Term Food Intake and Orexigenic Hypothalamic Responses in a Species with Continuous Nutrient Delivery
Authors: van Ackern Isabel , Kuhla Angela , Kuhla Björn
Published: October 2021

The endocannabinoid system (ECS) plays a pivotal role in the complex control and regulation of food intake. Pharmacological ECS activation could improve health in energy-deficient stages by increasing food intake, at least in intermittent feeders. However, knowledge of the mechanism regulating appetite in species with continued nutrient delivery is incomplete. The objectives of this pilot study were to investigate the effect of the intraperitoneal (i.p.) administration of the endocannabinoids (ECs) anandamide (AEA) and 2-arachidonoylglycerol (2-AG) on food intake, plasma EC concentrations and hypothalamic orexigenic signaling, and to study how the circulatory EC tone changes in response to short-term food deprivation in dairy cows, a species with continuous nutrient delivery. The administration of EC resulted in higher food intake during the first hour after treatment. Plasma AEA concentrations were significantly increased 2.5 h after AEA injection, whereas plasma 2-AG concentrations remained unchanged 2.5 h after 2-AG injection. The hypothalamic immunoreactivity of cannabinoid receptor 1, agouti-related protein, and orexin-A was not affected by either treatment; however, neuropeptide Y and agouti-related protein mRNA abundances were downregulated in the arcuate nucleus of AEA-treated animals. Short-term food deprivation increased plasma 2-AG, while plasma AEA remained unchanged. In conclusion, i.p.-administered 2-AG and AEA increase food intake in the short term, but only AEA accumulates in the circulation. However, plasma 2-AG concentrations are more responsive to food deprivation than AEA.
Nutrition Care Practices of Dietitians and Oral Health Professionals for Oral Health Conditions: A Scoping Review
Authors: Lieffers Jessica R. L. , Vanzan Amanda Gonçalves Troyack , Rover de Mello Janine , Cammer Allison
Published: October 2021

Background: Oral health conditions, such as dental caries, pose a substantial burden worldwide. Although there are many risk factors for poor oral health, diet is often implicated as a cause of these issues. The purpose of this scoping review was to identify and map studies that have captured information on the “real-world” nutrition care practices of oral health professionals (OHPs) and dietitians to optimize oral health, and specifically the dentition and periodontium. Methods: A search of peer-reviewed articles was conducted using MEDLINE, CINAHL, and Embase. Articles that addressed the review objective and met the following criteria were included: English language, published since 2000, and study conducted in a high-income country. Results: Overall, 70 articles were included. Most articles reported on cross-sectional survey studies and provided self-reported data on OHP practices; few articles reported on dietitians. Most articles reported only general/unspecific information on assessment and intervention practices, such as dietary analysis, nutrition counselling, and diet advice, and lacked specific information about the care provided, such as the dietary assessment tools used, type of information provided, and time spent on these activities. Barriers to the provision of nutrition care by OHPs were common and included time and lack of remuneration. Few studies reported on collaboration between dietitians and OHPs. Conclusions: Several studies have captured self-reported information on nutrition care practices of OHPs related to oral health; however, there is limited information available on the details of the care provided. Few studies have examined the practices of dietitians.
Gamification for Photonics Students: Labescape
Authors: Pérez-Herrera Rosa Ana , Tainta Santiago , Elosúa Cesar
Published: October 2021

The idea of utilizing game elements in non-gaming situations has sparked a lot of attention in recent years, especially in topics such as education and training. Game-based techniques appear to be an increasing trend in a wide range of learning areas, including health, social policy, and engineering, among others, not only in primary school but also in higher formal education. Using this methodology, the learning process becomes more stimulating while also reaching a competitive level in some circumstances. In the present work, the authors propose a new gamification strategy based on an escape-room in which all the puzzles to be passed are related to the area of optics and photonics and use readily available or low-cost equipment. The major field of application of this novel teaching strategy will be the practical section of a course, that is usually carried out in a laboratory, and will be aimed at both undergraduate and master’s degree students. A coevaluation method is also proposed where the rest of the students will provide valuable feedback to each one of their colleagues and to the instructor.
Boranils: Versatile Multifunctional Organic Fluorophores for Innovative Applications
Authors: Massue Julien , Jacquemin Denis , Ulrich Gilles
Published: October 2021

Multifunctional stimuli-responsive fluorophores showing bright environment-sensitive emissions have fueled intense research due to their innovative applications in the fields of biotechnologies, optoelectronics, and materials. A strong structural diversity is observed among molecular materials, which has been enriched over the years with a growing responsiveness to stimuli. Boron dipyrromethene (BODIPY) dyes have long been the flagship of emissive boron complexes due to their outstanding properties until a decade ago when analogues based on N^O, N^N, or N^C π-conjugated chelates emerged. The finality of developing borate dyes was to compensate for BODIPYs’ lack of solid-state fluorescence and small Stokes shifts while keeping their excellent optical properties in solution. Among them, the borate complexes based on a salicylaldimine ligand, called by the acronym boranils appear as the most promising, owing to their facile synthesis and dual-state emission properties. Boranil dyes have proven to be good alternatives to BODIPY dyes and have been applied in applications such as bioimaging, bioconjugation, and detection of biosubstrates. Meanwhile, ab initio calculations have rationalized experimental results and provided insightful feedback for future designs. This review article aims at providing a concise yet representative overview of the chemistry around the boranil core with the subsequent applications.
Biosecurity at Cattle Farms: Strengths, Weaknesses, Opportunities and Threats
Authors: Renault Véronique , Humblet Marie-France , Pham Phuong , Saegerman Claude
Published: October 2021

Biosecurity is a key component of any animal and public health strategy and disease prevention and control programs. This study reviewed the main findings of different studies implemented from 2015 to 2021 to analyse the biosecurity situation at Belgian cattle farms, including attitudes and behaviours of cattle farmers and rural veterinarians regarding biosecurity measures. Specifically, the objective was to perform a SWOT (strengths, weaknesses, opportunities and threats) analysis of the situation and propose a new conceptual framework improving the level of biosecurity in the cattle sector. Biosecurity in cattle farming remains relatively low and faces multiple challenges. Its future improvement requires the different stakeholders to agree on shared goals and objectives and to carefully consider animal, public and environmental health, as well as socioeconomic and cultural factors. Further cost efficiency studies are required to identify the most important biosecurity measures and convince the stakeholders of their utility and benefits. Cattle farmers rely mainly on rural veterinarians for technical guidance and consider them as trustful informants. To be more effective in promoting these good practices, rural veterinarians need a proper guidance from the authorities, a proper training on biosecurity and communication, as well as an enabling environment.
Proteomics of Host-Helminth Interactions
Authors: Robinson Mark W. , Cwiklinski Krystyna
Published: October 2021

Helminth infections in people contribute to the 1 [...]
Inhibition of 11β-HSD1 Ameliorates Cognition and Molecular Detrimental Changes after Chronic Mild Stress in SAMP8 Mice
Authors: Puigoriol-Illamola Dolors , Companys-Alemany Júlia , McGuire Kris , Homer Natalie Z. M. , Leiva Rosana , Vázquez Santiago , Mole Damian J. , Griñán-Ferré Christian , Pallàs Mercè
Published: October 2021

Impaired glucocorticoid (GC) signaling is a significant factor in aging, stress, and neurodegenerative diseases such as Alzheimer’s disease. Therefore, the study of GC-mediated stress responses to chronic moderately stressful situations, which occur in daily life, is of huge interest for the design of pharmacological strategies toward the prevention of neurodegeneration. To address this issue, SAMP8 mice were exposed to the chronic mild stress (CMS) paradigm for 4 weeks and treated with RL-118, an 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) inhibitor. The inhibition of this enzyme is linked with a reduction in GC levels and cognitive improvement, while CMS exposure has been associated with reduced cognitive performance. The aim of this project was to assess whether RL-118 treatment could reverse the deleterious effects of CMS on cognition and behavioral abilities and to evaluate the molecular mechanisms that compromise healthy aging in SAMP8 mice. First, we confirmed the target engagement between RL-118 and 11β-HSD1. Additionally, we showed that DNA methylation, hydroxymethylation, and histone phosphorylation were decreased by CMS induction, and increased by RL-118 treatment. In addition, CMS exposure caused the accumulation of reactive oxygen species (ROS)-induced damage and increased pro-oxidant enzymes—as well as pro-inflammatory mediators—through the NF-κB pathway and astrogliosis markers, such as GFAP. Of note, these modifications were reversed by 11β-HSD1 inhibition. Remarkably, although CMS altered mTORC1 signaling, autophagy was increased in the SAMP8 RL-118-treated mice. We also showed an increase in amyloidogenic processes and a decrease in synaptic plasticity and neuronal remodeling markers in mice under CMS, which were consequently modified by RL-118 treatment. In conclusion, 11β-HSD1 inhibition through RL-118 ameliorated the detrimental effects induced by CMS, including epigenetic and cognitive disturbances, indicating that GC-excess attenuation shows potential as a therapeutic strategy for age-related cognitive decline and AD.
AS1411 Aptamer Linked to DNA Nanostructures Diverts Its Traffic Inside Cancer Cells and Improves Its Therapeutic Efficacy
Authors: Vindigni Giulia , Raniolo Sofia , Iacovelli Federico , Unida Valeria , Stolfi Carmine , Desideri Alessandro , Biocca Silvia
Published: October 2021

The nucleolin-binding G-quadruplex AS1411 aptamer has been widely used for cancer therapy and diagnosis and linked to nanoparticles for its selective targeting activity. We applied a computational and experimental integrated approach to study the effect of engineering AS1411 aptamer on an octahedral truncated DNA nanocage to obtain a nanostructure able to combine selective cancer-targeting and anti-tumor activity. The nanocages functionalized with one aptamer molecule (Apt-NC) displayed high stability in serum, were rapidly and selectively internalized in cancer cells through an AS1411-dependent mechanism, and showed over 200-fold increase in anti-cancer activity when compared with the free aptamer. Comparison of Apt-NCs and free AS1411 intracellular distribution showed that they traffic differently inside cells: Apt-NCs distributed through the endo-lysosomal pathway and were never found in the nuclei, while the free AS1411 was mostly found in the perinuclear region and in nucleoli. Molecular dynamics simulations indicated that the aptamer, when linked to the nanocage, sampled a limited conformational space, more confined than in the free state, which is characterized by a large number of metastable conformations. A different intracellular trafficking of Apt-NCs compared with free aptamer and the confined aptamer conformations induced by the nanocage were likely correlated with the high cytotoxic enhancement, suggesting a structure–function relationship for the AS1411 aptamer activity.
Design, Synthesis, Characterization, and In Vitro Evaluation of a New Cross-Linked Hyaluronic Acid for Pharmaceutical and Cosmetic Applications
Authors: Sciabica Sabrina , Tafuro Giovanni , Semenzato Alessandra , Traini Daniela , Silva Dina M. , Reis Larissa Gomes Dos , Canilli Luisa , Terno Massimo , Durini Elisa , Vertuani Silvia , Baldisserotto Anna , Manfredini Stefano
Published: October 2021

Hyaluronic acid (HA), an excellent biomaterial with unique bio properties, is currently one of the most interesting polymers for many biomedical and cosmetic applications. However, several of its potential benefits are limited as it is rapidly degraded by hyaluronidase enzymes. To improve the half-life and consequently increase performance, native HA has been modified through cross-linking reactions with a natural and biocompatible amino acid, Ornithine, to overcome the potential toxicity commonly associated with traditional linkers. 2-chloro-dimethoxy-1,3,5-triazine/4-methylmorpholine (CDMT/NMM) was used as an activating agent. The new product (HA–Orn) was extensively characterized to confirm the chemical modification, and rheological analysis showed a gel-like profile. In vitro degradation experiments showed an improved resistance profile against enzymatic digestions. Furthermore, in vitro cytotoxicity studies were performed on lung cell lines (Calu-3 and H441), which showed no cytotoxicity.