bluuebunny/crossref_metadata_2025 huggingface dataset:
Arterial injuries after penetrating brain injury in civilians: risk factors on admission head computed tomography
Authors: Bodanapally Uttam K. , Saksobhavivat Nitima , Shanmuganathan Kathirkamanathan , Aarabi Bizhan , Roy Ashis K.
Published: October 2014

OBJECT

The object of this study was to determine the specific CT findings of the injury profile in penetrating brain injury (PBI) that are risk factors related to intracranial arterial injuries.

METHODS

The authors retrospectively evaluated admission head CTs and accompanying digital subtraction angiography (DSA) studies from patients with penetrating trauma to the head in the period between January 2005 and December 2012. Two authors reviewed the CT images to determine the presence or absence of 30 injury profile variables and quantified selected variables. The CT characteristics in patients with and without arterial injuries were compared using univariate analysis, multivariate analysis, and receiver operating characteristic (ROC) curve analysis to determine the respective risk factors, independent predictors, and optimal threshold values for the continuous variables.

RESULTS

Fifty-five patients were eligible for study inclusion. The risk factors for an intracranial arterial injury on univariate analysis were an entry wound over the frontobasal-temporal regions, a bihemispheric wound trajectory, a wound trajectory in proximity to the circle of Willis (COW), a subarachnoid hemorrhage (SAH), a higher SAH score, an intraventricular hemorrhage (IVH), and a higher IVH score. A trajectory in proximity to the COW was the best predictor of injury (OR 6.8 and p = 0.005 for all penetrating brain injuries [PBIs]; OR 13.3 and p = 0.001 for gunshot wounds [GSWs]). Significant quantitative variables were higher SAH and IVH scores. An SAH score of 3 (area under the ROC curve [AUC] for all PBIs 0.72; AUC for GSWs 0.71) and an IVH score of 3 (AUC for all PBIs 0.65; AUC for GSWs 0.65) could be used as threshold values to suggest an arterial injury.

CONCLUSIONS

The risk factors identified may help radiologists suggest the possibility of arterial injury and prioritize neurointerventional consultation and potential DSA studies.
Sinus pericranii: diagnosis and management in 21 pediatric patients
Authors: Pavanello Marco , Melloni Ilaria , Antichi Eleonora , Severino Mariasavina , Ravegnani Marcello , Piatelli Gianluca , Cama Armando , Rossi Andrea , Gandolfo Carlo
Published: October 2014

OBJECT

Sinus pericranii (SP) is a rare venous anomaly abnormally connecting the intracranial dural sinuses with the epicranial veins. In the present study the authors aimed to clarify this clinicoradiological entity, define the role of angiography in its preoperative assessment, and suggest a diagnostic-therapeutic flow chart for management purposes.

METHODS

The authors retrospectively reviewed the clinical charts and neuroimages of 21 patients with SP. All patients underwent brain MRI, MR venography, and craniocerebral CT. Diagnostic digital subtraction angiography was performed in 19 of 21 patients, and the SPs were categorized as dominant (draining the majority of the intracranial venous outflow) or accessory (draining only a minority of the intracranial venous outflow).

RESULTS

SP was median or paramedian in 20 patients and lateral in 1 patient. There were 5 dominant and 14 accessory SPs. The dominant SPs were not treated. Among the patients with accessory SP, 4 were not treated, 2 underwent surgical ligature, and 8 were treated endovascularly (with either transvenous or percutaneous embolization). No complications were observed, and symptoms disappeared after treatment in all cases.

CONCLUSIONS

Accepted guidelines or recommendations concerning the management, diagnosis, and treatment of SP are still lacking. The authors define here a diagnostic-therapeutic flow chart, in which angiography plays a crucial role in the classification of SP and choice of the optimal treatment. Only accessory SP is amenable to treatment, whereas dominant SP must be preserved. The endovascular approach is becoming increasingly relevant and has proven to be safe and effective.
Targeted intraarterial anti-VEGF therapy for medically refractory radiation necrosis in the brain
Authors: Dashti Shervin R. , Spalding Aaron , Kadner Rob J. , Yao Tom , Kumar Arooshi , Sun David A. , LaRocca Renato
Published: October 2014

Radiation necrosis (RN) is a serious complication that can occur in up to 10% of brain radiotherapy cases, with the incidence dependent on both dose and brain location. Available medical treatment for RN includes steroids, vitamin E, pentoxifylline, and hyperbaric oxygen. In a significant number of patients, however, RN is medically refractory and the patients experience progressive neurological decline, disabling headaches, and decreased quality of life.

Vascular endothelial growth factor (VEGF) is a known mediator of cerebral edema in RN. Recent reports have shown successful treatment of RN with intravenous bevacizumab, a monoclonal antibody for VEGF. Bevacizumab, however, is associated with significant systemic complications including sinus thrombosis, pulmonary embolus, gastrointestinal tract perforation, wound dehiscence, and severe hypertension. Using lower drug doses may decrease systemic exposure and reduce complication rates. By using an intraarterial route for drug administration following blood-brain barrier disruption (BBBD), the authors aim to lower the bevacizumab dose while increasing target delivery.

In the present report, the authors present the cases of 2 pediatric patients with cerebral arteriovenous malformations, who presented with medically intractable RN following stereotactic radiosurgery. They received a single intraarterial infusion of 2.5 mg/kg bevacizumab after hyperosmotic BBBD.

At mean follow-up duration of 8.5 months, the patients had significant and durable clinical and radiographic response. Both patients experienced resolution of their previously intractable headaches and reversal of cushingoid features as they were successfully weaned off steroids. One of the patients regained significant motor strength. There was an associated greater than 70% reduction in cerebral edema.

Intraarterial administration of a single low dose of bevacizumab after BBBD was safe and resulted in durable clinical and radiographic improvements at concentrations well below those required for the typical systemic intravenous route. Advantages over the intravenous route may include higher concentration of drug delivery to the affected brain, decreased systemic toxicity, and a significantly lower cost.
Design and evaluation of a new synthetic brain simulator for endoscopic third ventriculostomy
Authors: Breimer Gerben E. , Bodani Vivek , Looi Thomas , Drake James M.
Published: October 2014

OBJECT

Endoscopic third ventriculostomy (ETV) is an effective but technically demanding procedure with significant risk. Current simulators, including human cadavers, animal models, and virtual reality systems, are expensive, relatively inaccessible, and can lack realistic sensory feedback. The purpose of this study was to construct a realistic, low-cost, reusable brain simulator for ETV and evaluate its fidelity.

METHODS

A brain silicone replica mimicking normal mechanical properties of a 4-month-old child with hydrocephalus was constructed, encased in the replicated skull, and immersed in water. Realistic intraventricular landmarks included the choroid plexus, veins, mammillary bodies, infundibular recess, and basilar artery. The thinned-out third ventricle floor, which dissects appropriately, is quickly replaceable. Standard neuroendoscopic equipment including irrigation is used. Bleeding scenarios are also incorporated. A total of 16 neurosurgical trainees (Postgraduate Years 1–6) and 9 pediatric and adult neurosurgeons tested the simulator. All participants filled out questionnaires (5-point Likert-type items) to rate the simulator for face and content validity.

RESULTS

The simulator is portable, robust, and sets up in minutes. More than 95% of participants agreed or strongly agreed that the simulator's anatomical features, tissue properties, and bleeding scenarios were a realistic representation of that seen during an ETV. Participants stated that the simulator helped develop the required hand-eye coordination and camera skills, and the training exercise was valuable.

CONCLUSIONS

A low-cost, reusable, silicone-based ETV simulator realistically represents the surgical procedure to trainees and neurosurgeons. It can help them develop the technical and cognitive skills for ETV including dealing with complications.
Evaluation of glycerol-preserved bone allografts in cervical spine fusion: a prospective, randomized controlled trial
Authors: Graham R. Scott , Samsell Brian J. , Proffer Allison , Moore Mark A. , Vega Rafael A. , Stary Joel M. , Mathern Bruce
Published: October 2014

OBJECT

Bone allografts used for interbody spinal fusion are often preserved through either freeze drying or lowtemperature freezing, each having disadvantages related to graft preparation time and material properties. In response, a glycerol preservation treatment has been developed to maintain the biomechanical properties of allografts at ambient temperatures, requiring no thawing or rehydration and minimal rinsing prior to implantation. The authors conducted a prospective randomized study to compare the clinical results of glycerol-preserved Cloward dowels and those of freezedried Cloward dowels in anterior cervical discectomy and fusion. The primary outcome measures were evidence of fusion and graft subsidence, and the secondary outcome measures included adverse events, pain, and neck disability scores.

METHODS

Of 106 patients, 53 (113 levels of surgery) were randomly assigned to the glycerol-preserved graft group and 53 (114 levels of surgery) to the freeze-dried graft group. Subsidence was assessed at 3 and 6 months after implantation. Evidence of fusion was evaluated radiographically at 6 months postimplantation. Subsidence was quantitatively assessed based on physical measurements obtained from radiographs by using calibrated comparators, whereas fusion was also evaluated visually. Surgeons were blinded to treatment type during visual and physical assessments of the patients and the radiographs.

RESULTS

No one in either group had evidence of complete nonunion according to radiographic evaluation at the 6-month follow-up. Average subsidence for all graft-treated levels was 2.11 mm for the glycerol-preserved group and 2.73 mm for the freeze-dried group at the 3-month follow-up and 2.13 and 2.83 mm at the 6-month follow-up, respectively. The 2 treatment groups were statistically equivalent (p = 0.2127 and 0.1705 for the 3- and 6-month follow-up, respectively). No differences were noted between the graft types in terms of adverse event incidence or severity.

CONCLUSIONS

Glycerol-preserved bone allografts exhibit fusion results and subsidence values similar to those of their freeze-dried counterparts, potentially more favorable biomechanical properties, and significantly shorter preparation times.
Migrating lumbar intrathecal catheter fragment associated with intracranial subarachnoid hemorrhage
Authors: Hnenny Luke , Sabry Hatem A. , Raskin Jeffrey S. , Liu Jesse J. , Roundy Neil E. , Dogan Aclan
Published: October 2014

Intrathecal catheter placement into the lumbar cistern has varied indications, including drug delivery and CSF diversion. These Silastic catheters are elastic and durable; however, catheter-associated malfunctions are well reported in the literature. Fractured catheters are managed with some variability, but entirely intradural retained fragments are often managed conservatively with observation.

The authors describe a case of a 70-year-old man with an implanted intrathecal morphine pump for failed back surgery syndrome who presented to an outside hospital with a history of headache, neck pain, nausea, and photophobia of 3 days' duration. He also described mild weakness and intermittent numbness of both legs. Unenhanced head CT demonstrated subarachnoid hemorrhage (SAH). A right C-5 hemilaminectomy was performed.

This case is unique in that there was no indication that the lumbar intrathecal catheter had fractured prior to the patient's presentation with SAH. This case demonstrates that intrathecal catheter fragments are mobile and can precipitate intracranial morbidity. Extrication of known fragments is safe and should be attempted to prevent further neurosurgical morbidity.
Deleuze, Darwin and the Categorisation of Life
Authors: Eckstrand Nathan
Published: October 2014

I begin with Deleuze's criticism of the Darwinian concept of difference as leading to the inaccurate assumption that difference occurs within individuals and species. Deleuze radicalises Darwin's theory by disrupting the ontological stability of species and extant individualities. I examine how Deleuze's project relates to punctuated equilibrium and the discovery of the amount of variation within the human genome, showing that these recent developments make Deleuze's critique less applicable by showing that Darwinian classification schemes should include a greater openness to difference. A complete alignment between evolutionary biology and Deleuze may be impossible given the limitations of evolutionary biology, but evolutionary biology can rethink the ontological permanence it gives to species and individuals.
The Rise and Fall of the Simulacrum
Authors: Mayell Charles
Published: October 2014

Deleuze adopts Nietzsche's manifesto for an overturning of Platonism. However, the consensus view is that Deleuze's project is best understood as a revision not a repudiation of Platonism. Deleuze's engagement with Platonism centres on The Sophist. Out of Plato's concept of phantasm, Deleuze fashions a new concept: simulacrum. In Difference and Repetition, simulacra are invited to rise and affirm their rights; and yet Deleuze later abandons the concept entirely. Why? Although suitable for the purposes of critique, it became otiose in wider applications. More generally, and against the consensus view, I argue that the trajectory of the concept of the simulacrum is emblematic of Deleuze's anti-Platonism.
Scoring the Rhizome: Bussotti's Musical Diagram
Authors: Bogue Ronald
Published: October 2014

The score of Piece Four of Sylvano Bussotti's Five Piano Pieces for David Tudor is the most important image in A Thousand Plateaus. It serves as a prefatory image not only to the Rhizome plateau, but also to the work as a whole. It functions as the book's musical score, guiding readers in their performance of the text. Embracing John Cage's graphism and aleatory practices, Bussotti created his own ‘aserial’ new music, one that celebrated passion and Bussotti's open homosexuality. The visual elements of Piece Four include a deterritorialisation of the standard piano score, a diagram of the composition's abstract machine, and a drawing that Bussotti had produced ten years before writing Five Piano Pieces for David Tudor. The drawing itself is a rhizomic artwork, with details that echo visual motifs throughout A Thousand Plateaus. The superimposition of the drawing on the deterritorialised framework of the standard piano score conjoins the visible and the audible, faciality and the refrain, in a single artefact.
Design of Dwellings and Interior Family Space in China: Understanding the History of Change and Opportunities for Improved Sustainability Practices
Authors: Pitts Adrian , Gao Yun
Published: October 2014

This paper reviews briefly the recent history of dwelling design in China. It notes the rapid changes that have taken place since the 1980s and identifies the way contemporary procurement processes leave out the final fit-out and decoration/refurbishment. A range of stakeholders were interviewed, and access was gained to drawings and other technical data that indicated how the secondary processes were carried out. These are largely ungoverned by regulation in the same way necessary for initial design. The key group is the occupants who drive the fit-out and decoration according to personal and cultural requirements, but often with less than perfect understanding of sustainability. The interior design industry has developed rapidly over the same period and was initially lacking in professional knowledge and understanding (something which can still be found). Advice provided to dwelling occupants was based more on appearance than function and efficiency. Over the same period, beneficial modifications to construction processes have been introduced in relation to structural design, and it should be possible to do the same for sustainability-related design issues. The paper advocates: more regulation; better assessment techniques; more information and guidance for home-owners; and a greater focus on energy issues.
Advances in Packaging Methods, Processes and Systems
Authors: Mahalik Nitaigour
Published: October 2014

The food processing and packaging industry is becoming a multi-trillion dollar global business. The reason is that the recent increase in incomes in traditionally less economically developed countries has led to a rise in standards of living that includes a significantly higher consumption of packaged foods. As a result, food safety guidelines have been more stringent than ever. At the same time, the number of research and educational institutions—that is, the number of potential researchers and stakeholders—has increased in the recent past. This paper reviews recent developments in food processing and packaging (FPP), keeping in view the aforementioned advancements and bearing in mind that FPP is an interdisciplinary area in that materials, safety, systems, regulation, and supply chains play vital roles. In particular, the review covers processing and packaging principles, standards, interfaces, techniques, methods, and state-of-the-art technologies that are currently in use or in development. Recent advances such as smart packaging, non-destructive inspection methods, printing techniques, application of robotics and machineries, automation architecture, software systems and interfaces are reviewed.
The Gender Digital Divide in Developing Countries
Authors: Antonio Amy , Tuffley David
Published: October 2014

Empirical studies clearly show that women in the developing world have significantly lower technology participation rates than men; a result of entrenched socio-cultural attitudes about the role of women in society. However, as studies are beginning to show, when those women are able to engage with Internet technology, a wide range of personal, family and community benefits become possible. The key to these benefits is on-line education, the access to which sets up a positive feedback loop. This review gives an overview of the digital divide, before focusing specifically on the challenges women in developing countries face in accessing the Internet. Current gender disparities in Internet use will be outlined and the barriers that potentially hinder women’s access and participation in the online world will be considered. We will then look at the potential opportunities for women’s participation in a global digital society along with a consideration of current initiatives that have been developed to mitigate gender inequity in developing countries. We will also consider a promising avenue for future research.
Comparative Proteomic Analysis of Labellum and Inner Lateral Petals in Cymbidium ensifolium Flowers
Authors: Li Xiaobai , Xu Weiwei , Chowdhury Moytri , Jin Feng
Published: October 2014

The labellum in orchids shares homology with the inner lateral petals of the flower. The labellum is a modified petal and often distinguished from other petals and sepals due to its large size and irregular shape. Herein, we combined two-dimensional gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization time of flight/time of flight (MALDI-TOF/TOF) approaches to identify the differentially expressed proteome between labellum and inner lateral petal in one of Orchid species (C. ensifolium). A total of 30 protein spots were identified, which showed more than a two-fold significant difference (p < 0.05) in their expression. Compared with C. ensifolium transcriptome (sequenced in house), 21 proteins matched the translated nucleotide. The proteins identified were classified into 48 categories according to gene ontology (GO). Additionally, these proteins were involved in 18 pathways and 9 possible protein-protein interactions. Serine carboxypeptidase and beta-glucosidase were involved in the phenylpropanoid pathway, which could regulate biosynthesis of floral scent components. Malate dehydrogenase (maeB) and triosephosphate isomerase (TPI) in carbon fixation pathway could regulate the energy metabolism. Xyloglucan endotransglucosylase/hydrolase (XET/XTH) could promote cell wall formation and aid the petal’s morphogenesis. The identification of such differentially expressed proteins provides new targets for future studies; these will assess the proteins’ physiological roles and significance in labellum and inner lateral petals.
Attachment of Luminescent Neutral “Pt(pq)(C≡CtBu)” Units to Di and Tri N-Donor Connecting Ligands: Solution Behavior and Photophysical Properties
Authors: Lalinde Elena , Moreno M. , Ruiz Santiago , Sánchez Sergio
Published: October 2014

Binuclear derivatives [{Pt(pq)(C≡CtBu)}2(μ-L)] (1a–5a), containing a series of dinitrogen linker ligands and the trinuclear [{Pt(pq)(C≡CtBu)}3(μ-L)] (6a) [L = μ-1,3,5-tris(pyridine-4-ylethynyl)benzene], formed by bridge-splitting reactions with [Pt(pq)(μ-κCα:η2-C≡CtBu)]2 (Pt-1), are reported. The complexes are characterized by a combination of 1H NMR spectroscopy, mass spectrometry and X-ray crystallography (2a and 4a). 1H NMR proves the existence of a dynamic equilibrium in solution between the diplatinum complexes (species a), the corresponding mononuclear complex with terminal N-donor ligands (species b), the starting material (Pt-1) and the free ligand (L). The effects of concentration, temperature and solvent properties on the equilibrium have been studied. The optical properties of these systems have been investigated by UV-visible absorption and emission spectroscopies in solid state and in solution, and the nature of the transitions and the excited state analyzed by theoretical calculations on 2a.
A Hydrido η1-Alkynyl Diplatinum Complex Obtained from a Phosphinito Phosphanido Complex and Trimethylsilylacetylene
Authors: Latronico Mario , Gallo Vito , Lalinde Elena , Ruiz Santiago , Todisco Stefano , Mastrorilli Piero
Published: October 2014

The reaction of (trimethylsilyl)acetylene with the phosphinito phosphanido Pt(I) complex [(PHCy2)Pt(μ-PCy2){κ2P,O-μ-P(O)Cy2}Pt(PHCy2)](Pt-Pt) (1) results in the protonation of the Pt-Pt bond with the formation of the bridging hydride complex [(PHCy2)(Me3SiC≡C)Pt(μ-PCy2)(μ-H) Pt(PHCy2){κP-P(O)Cy2}](Pt-Pt) (2), which was characterized by spectroscopic, spectrometric and XRD analyses. Complex 2 exhibits in the solid state at 77 K a long-lived, weak, orange emission assigned as metal-metal to ligand charge transfer (MMLCT) (L = alkynyl) due to the presence of a very short Pt···Pt distance [2.8209(2) Å]. Reaction of 2 with etherate HBF4 results in the selective protonation of the phosphinito ligand to afford the species [(PHCy2)(Me3SiC≡C)Pt(μ-PCy2)(μ-H) Pt(PHCy2){κP-P(OH)Cy2}](Pt-Pt)[BF4] ([3]BF4).
A Comparison of Female Delinquents: The Impact of Child Maltreatment Histories on Risk and Need Characteristics among a Missouri Sample
Authors: Dannerbeck-Janku Anne , Peters Clark , Perkins Jacob
Published: October 2014

While boys who offend have been a dominant majority and primary concern of the juvenile court since its earliest days, the population of delinquent girls has increased in recent years at a far higher rate in the U.S. The special challenges presented by females, however, continue to be generally overlooked by the justice system. Moreover, while a few specialized programs now serve these girls, the field tends to view young female offenders as a homogeneous group; what distinguishes particular female subpopulations and the characteristics associated with different criminal trajectories have gone largely unexplored. Employing data from the state of Missouri, this study examines girls who offend, identifying models that predict subsequent violent behavior that include indicators such as parental substance abuse and incarceration, and offender substance abuse, mental health, and school behavior. Special attention is given to the effects of child maltreatment, which we find significantly, but weakly correlated with violent behavior. The authors conclude by considering the possibility that maltreatment may be correlated with other criminogenic factors, and by discussing the implications of findings for future research and practice, especially services that take into account the trauma experienced by young women who come to the attention of state authorities.
Flavonoids from Gynostemma pentaphyllum Exhibit Differential Induction of Cell Cycle Arrest in H460 and A549 Cancer Cells
Authors: Tsui Ko-Chung , Chiang Tzu-Hsuan , Wang Jinn-Shyan , Lin Li-Ju , Chao Wei-Chih , Chen Bing-Huei , Lu Jyh-Feng
Published: October 2014

Flavonoids, containing mainly kaempferol rhamnohexoside derivatives, were extracted from Gynostemma pentaphyllum (G. pentaphyllum) and their potential growth inhibition effects against H460 non-small cell lung cancer cells was explored and compared to that on A549 cells. The extracted flavonoids were found to exhibit antiproliferation effects against H460 cells (IC50 = 50.2 μg/mL), although the IC50 of H460 is 2.5-fold that of A549 cells (IC50 = 19.8 μg/mL). Further investigation revealed that H460 cells are more susceptible to kaempferol than A549, whereas A549 cell growth is better inhibited by kaempferol rhamnohexoside derivatives as compared with H460. In addition, flavonoids from G. pentaphyllum induced cell cycle arrest at both S and G2/M phases with concurrent modulated expression of the cellular proteins cyclin A, B, p53 and p21 in A549 cells, but not H460. On the contrary, apoptosis and concomitant alteration in balance of BCL-2 and BAX expression as well as activation of caspase-3 were equally affected between both cells by flavonoid treatment. These observations strongly suggest the growth inhibition discrepancy between H460 and A549 following flavonoid treatment can be attributed to the lack of cell cycle arrest in H460 cells and the differences between H460 and A549 cells may serve as contrasting models for further mechanistic investigations.
Tungsten Trioxide as a Visible Light Photocatalyst for Volatile Organic Carbon Removal
Authors: Wicaksana Yossy , Liu Sanly , Scott Jason , Amal Rose
Published: October 2014

Tungsten trioxide (WO3) has been demonstrated to possess visible light photoactivity and presents a means of overcoming the UV-light dependence of photocatalysts, such as titanium dioxide. In this study, WO3 nanostructures have been synthesised by a hydrothermal method using sodium tungstate (Na2WO4·2H2O), sulphate precursors and pH as structure-directing agents and parameters, respectively. By altering the concentration of the sulphate precursors and pH, it was shown that different morphologies and phases of WO3 can be achieved. The effect of the morphology of the final WO3 product on the visible light photoactivity of ethylene degradation in the gas phase was investigated. In addition, platinum (Pt) was photodeposited on the WO3 structures with various morphologies to enhance the photocatalytic properties. It was found that the photocatalytic properties of the WO3 samples greatly depend on their morphology, chemical composition and surface modification. WO3 with a cuboid morphology exhibited the highest visible light photoactivity compared to other morphologies, while adding Pt to the surface improved the performance of certain WO3 structures.
The Health Advantage of a Vegan Diet: Exploring the Gut Microbiota Connection
Authors: Glick-Bauer Marian , Yeh Ming-Chin
Published: October 2014

This review examines whether there is evidence that a strict vegan diet confers health advantages beyond that of a vegetarian diet or overall healthy eating. Few studies include vegan subjects as a distinct experimental group, yet when vegan diets are directly compared to vegetarian and omnivorous diets, a pattern of protective health benefits emerges. The relatively recent inclusion of vegan diets in studies of gut microbiota and health allows us the opportunity to assess whether the vegan gut microbiota is distinct, and whether the health advantages characteristic of a vegan diet may be partially explained by the associated microbiota profile. The relationship between diet and the intestinal microbial profile appears to follow a continuum, with vegans displaying a gut microbiota most distinct from that of omnivores, but not always significantly different from that of vegetarians. The vegan gut profile appears to be unique in several characteristics, including a reduced abundance of pathobionts and a greater abundance of protective species. Reduced levels of inflammation may be the key feature linking the vegan gut microbiota with protective health effects. However, it is still unclear whether a therapeutic vegan diet can be prescribed to alter the gut microflora for long-term health benefits.
An Analysis of the Side Slither On-Orbit Calibration Technique Using the DIRSIG Model
Authors: Gerace Aaron , Schott John , Gartley Michael , Montanaro Matthew
Published: October 2014

Pushbroom-style imaging systems exhibit several advantages over line scanners when used on space-borne platforms as they typically achieve higher signal-to-noise and reduce the need for moving parts. Pushbroom sensors contain thousands of detectors, each having a unique radiometric response, which will inevitably lead to streaking and banding in the raw data. To take full advantage of the potential exhibited by pushbroom sensors, a relative radiometric correction must be performed to eliminate pixel-to-pixel non-uniformities in the raw data. Side slither is an on-orbit calibration technique where a 90-degree yaw maneuver is performed over an invariant site to flatten the data. While this technique has been utilized with moderate success for the QuickBird satellite [1] and the RapidEye constellation [2], further analysis is required to enable its implementation for the Landsat 8 sensors, which have a 15-degree field-of-view and a 0.5% pixel-to-pixel uniformity requirement. This work uses the DIRSIG model to analyze the side slither maneuver as applicable to the Landsat sensor. A description of favorable sites, how to adjust the maneuver to compensate for the curvature of “linear” arrays, how to efficiently process the data, and an analysis to assess the quality of the side slither data, are presented.
Ground Surface Response to Geothermal Drilling and the Following Counteractions in Staufen im Breisgau (Germany) Investigated by TerraSAR-X Time Series Analysis and Geophysical Modeling
Authors: Lubitz Christin , Motagh Mahdi , Kaufmann Hermann
Published: October 2014

The city of Staufen im Breisgau in southwest Germany suffers from a localized land uplift, which has occurred in the past six years in relation to geothermal drilling activities in 2007. So far, severe damages at 269 buildings have been recorded. The chemical transformation of anhydrite and water to gypsum, resulting in a volume increase, has been attributed as the cause of the uplift. Previous studies provide knowledge on the spatio-temporal displacement evolution from 2008 through 2011 using leveling and spaceborne Synthetic Aperture Radar Interferometry (InSAR) measurements, but lack a detailed representation of vertical and horizontal displacement contributions as well as geophysical modeling. This study focuses not only on continued observation analysis from June 2011 through July 2013, but also on obtaining and evaluating horizontal displacements in Staufen based on combined analysis of TerraSAR-X satellite imagery from both ascending and descending orbits. Applying the Small BAseline Subset (SBAS) approach a deceleration of annual cumulative line of sight (LOS) uplift is observable from 13.8 cm ± 0.3 cm (July 2008–July 2009) to 3 cm ± 0.3 cm (July 2012–July 2013) within area of maximum deformation NNE of the drilling zone. Conducting displacement decomposition on ascending and descending data of a common period (October 2012 through July 2013) yields in an approximately symmetric east- and westward motion with maximum values approximately 1 cm and 1.4 cm, respectively. The joint inversion of ascending and descending InSAR data for the common period from October 2012 through July 2013 shows that a horizontal rectangular source with length, width and depth of 177 m ± 19 m, 69 m ± 15 m and 89 m ± 9 m, respectively, can satisfactorily model the observation. The amount of opening at depth shows a decrease in time by about 71% for the period 2011–2012 as compared to period 2008–2009.
Automatic Classification of the Sub-Techniques (Gears) Used in Cross-Country Ski Skating Employing a Mobile Phone
Authors: Stöggl Thomas , Holst Anders , Jonasson Arndt , Andersson Erik , Wunsch Tobias , Norström Christer , Holmberg Hans-Christer
Published: October 2014

The purpose of the current study was to develop and validate an automatic algorithm for classification of cross-country (XC) ski-skating gears (G) using Smartphone accelerometer data. Eleven XC skiers (seven men, four women) with regional-to-international levels of performance carried out roller skiing trials on a treadmill using fixed gears (G2left, G2right, G3, G4left, G4right) and a 950-m trial using different speeds and inclines, applying gears and sides as they normally would. Gear classification by the Smartphone (on the chest) and based on video recordings were compared. Formachine-learning, a collective database was compared to individual data. The Smartphone application identified the trials with fixed gears correctly in all cases. In the 950-m trial, participants executed 140 ± 22 cycles as assessed by video analysis, with the automatic Smartphone application giving a similar value. Based on collective data, gears were identified correctly 86.0% ± 8.9% of the time, a value that rose to 90.3% ± 4.1% (P < 0.01) with machine learning from individual data. Classification was most often incorrect during transition between gears, especially to or from G3. Identification was most often correct for skiers who made relatively few transitions between gears. The accuracy of the automatic procedure for identifying G2left, G2right, G3, G4left and G4right was 96%, 90%, 81%, 88% and 94%, respectively. The algorithm identified gears correctly 100% of the time when a single gear was used and 90% of the time when different gears were employed during a variable protocol. This algorithm could be improved with respect to identification of transitions between gears or the side employed within a given gear.
Chronically Implanted Pressure Sensors: Challenges and State of the Field
Authors: Yu Lawrence , Kim Brian , Meng Ellis
Published: October 2014

Several conditions and diseases are linked to the elevation or depression of internal pressures from a healthy, normal range, motivating the need for chronic implantable pressure sensors. A simple implantable pressure transduction system consists of a pressure-sensing element with a method to transmit the data to an external unit. The biological environment presents a host of engineering issues that must be considered for long term monitoring. Therefore, the design of such systems must carefully consider interactions between the implanted system and the body, including biocompatibility, surgical placement, and patient comfort. Here we review research developments on implantable sensors for chronic pressure monitoring within the body, focusing on general design requirements for implantable pressure sensors as well as specifications for different medical applications. We also discuss recent efforts to address biocompatibility, efficient telemetry, and drift management, and explore emerging trends.
Monitoring and Control Interface Based on Virtual Sensors
Authors: Escobar Ricardo , Adam-Medina Manuel , García-Beltrán Carlos , Olivares-Peregrino Víctor , Juárez-Romero David , Guerrero-Ramírez Gerardo
Published: October 2014

In this article, a toolbox based on a monitoring and control interface (MCI) is presented and applied in a heat exchanger. The MCI was programed in order to realize sensor fault detection and isolation and fault tolerance using virtual sensors. The virtual sensors were designed from model-based high-gain observers. To develop the control task, different kinds of control laws were included in the monitoring and control interface. These control laws are PID, MPC and a non-linear model-based control law. The MCI helps to maintain the heat exchanger under operation, even if a temperature outlet sensor fault occurs; in the case of outlet temperature sensor failure, the MCI will display an alarm. The monitoring and control interface is used as a practical tool to support electronic engineering students with heat transfer and control concepts to be applied in a double-pipe heat exchanger pilot plant. The method aims to teach the students through the observation and manipulation of the main variables of the process and by the interaction with the monitoring and control interface (MCI) developed in LabVIEW©. The MCI provides the electronic engineering students with the knowledge of heat exchanger behavior, since the interface is provided with a thermodynamic model that approximates the temperatures and the physical properties of the fluid (density and heat capacity). An advantage of the interface is the easy manipulation of the actuator for an automatic or manual operation. Another advantage of the monitoring and control interface is that all algorithms can be manipulated and modified by the users.
PhTX-II a Basic Myotoxic Phospholipase A2 from Porthidium hyoprora Snake Venom, Pharmacological Characterization and Amino Acid Sequence by Mass Spectrometry
Authors: Huancahuire-Vega Salomón , Ponce-Soto Luis , Marangoni Sergio
Published: October 2014

A monomeric basic PLA2 (PhTX-II) of 14149.08 Da molecular weight was purified to homogeneity from Porthidium hyoprora venom. Amino acid sequence by in tandem mass spectrometry revealed that PhTX-II belongs to Asp49 PLA2 enzyme class and displays conserved domains as the catalytic network, Ca2+-binding loop and the hydrophobic channel of access to the catalytic site, reflected in the high catalytic activity displayed by the enzyme. Moreover, PhTX-II PLA2 showed an allosteric behavior and its enzymatic activity was dependent on Ca2+. Examination of PhTX-II PLA2 by CD spectroscopy indicated a high content of alpha-helical structures, similar to the known structure of secreted phospholipase IIA group suggesting a similar folding. PhTX-II PLA2 causes neuromuscular blockade in avian neuromuscular preparations with a significant direct action on skeletal muscle function, as well as, induced local edema and myotoxicity, in mice. The treatment of PhTX-II by BPB resulted in complete loss of their catalytic activity that was accompanied by loss of their edematogenic effect. On the other hand, enzymatic activity of PhTX-II contributes to this neuromuscular blockade and local myotoxicity is dependent not only on enzymatic activity. These results show that PhTX-II is a myotoxic Asp49 PLA2 that contributes with toxic actions caused by P. hyoprora venom.
Uptake and Processing of the Cytolethal Distending Toxin by Mammalian Cells
Authors: DiRienzo Joseph
Published: October 2014

The cytolethal distending toxin (Cdt) is a heterotrimeric holotoxin produced by a diverse group of Gram-negative pathogenic bacteria. The Cdts expressed by the members of this group comprise a subclass of the AB toxin superfamily. Some AB toxins have hijacked the retrograde transport pathway, carried out by the Golgi apparatus and endoplasmic reticulum (ER), to translocate to cytosolic targets. Those toxins have been used as tools to decipher the roles of the Golgi and ER in intracellular transport and to develop medically useful delivery reagents. In comparison to the other AB toxins, the Cdt exhibits unique properties, such as translocation to the nucleus, that present specific challenges in understanding the precise molecular details of the trafficking pathway in mammalian cells. The purpose of this review is to present current information about the mechanisms of uptake and translocation of the Cdt in relation to standard concepts of endocytosis and retrograde transport. Studies of the Cdt intoxication process to date have led to the discovery of new translocation pathways and components and most likely will continue to reveal unknown features about the mechanisms by which bacterial proteins target the mammalian cell nucleus. Insight gained from these studies has the potential to contribute to the development of novel therapeutic strategies.
Integration Site and Clonal Expansion in Human Chronic Retroviral Infection and Gene Therapy
Authors: Niederer Heather , Bangham Charles
Published: October 2014

Retroviral vectors have been successfully used therapeutically to restore expression of genes in a range of single-gene diseases, including several primary immunodeficiency disorders. Although clinical trials have shown remarkable results, there have also been a number of severe adverse events involving malignant outgrowth of a transformed clonal population. This clonal expansion is influenced by the integration site profile of the viral integrase, the transgene expressed, and the effect of the viral promoters on the neighbouring host genome. Infection with the pathogenic human retrovirus HTLV-1 also causes clonal expansion of cells containing an integrated HTLV-1 provirus. Although the majority of HTLV-1-infected people remain asymptomatic, up to 5% develop an aggressive T cell malignancy. In this review we discuss recent findings on the role of the genomic integration site in determining the clonality and the potential for malignant transformation of cells carrying integrated HTLV-1 or gene therapy vectors, and how these results have contributed to the understanding of HTLV-1 pathogenesis and to improvements in gene therapy vector safety.
Levels of procoagulant microvesicles are elevated after traumatic injury and platelet microvesicles are negatively correlated with mortality
Authors: Curry Nicola , Raja Amber , Beavis James , Stanworth Simon , Harrison Paul
Published: October 2014

Background

Microvesicles (MV) have been implicated in the development of thrombotic disease, such as acute respiratory distress syndrome (ARDS) and multiple organ failure (MOF). Trauma patients are at increased risk of late thrombotic events, particularly those who receive a major transfusion. The aims of this study were: (a) to determine whether there were increased numbers of pro‐coagulant MV following injury; (b) to determine their cellular origin; and (c) to explore the effects of MV with clinical outcomes; in particular red cell transfusion requirements and death.

Methods

Trauma patients were recruited at a Level 1 trauma centre. The presence of MV procoagulant phospholipid (PPL) was assessed using 2 activity assays (PPL and thrombin generation). Enumeration and MV cellular origin was assessed using 2 colour flow cytometry.

Results

Fifty consecutive patients were recruited; median age 38 (IQR: 24–55), median ISS 18 (IQR: 9–27). Circulating procoagulant MV, rich in phospholipid, were significantly elevated following traumatic injury relative to controls and remained elevated at 72 h post‐injury. Red cell/AnnV+ and platelet/AnnV+ MV numbers were 6‐fold and 2‐fold higher than controls, respectively. Patients who died (n=9, 18%) had significantly fewer CD41/AnnV+ MV and lower endogenous thrombin potential relative to patients who survived.

Conclusions

MV are elevated following traumatic injury and may be implicated in the increased risk of trauma patients to pro‐thrombotic states such as MOF and ARDS. Lower levels of procoagulant MV are associated with mortality and further investigation of this association is warranted.
Effects of functionally asexual reproduction on quantitative genetic variation in the evening primroses (Oenothera, Onagraceae)
Authors: Godfrey Ryan M. , Johnson Marc T. J.
Published: October 2014

• Premise of the study: It has long been predicted that a loss of sexual reproduction leads to decreased heritable variation within populations and increased differentiation between populations. Despite an abundance of theory, there are few empirical tests of how sex affects genetic variation in phenotypic traits, especially for plants. Here we test whether repeated losses of two critical components of sex (recombination and segregation) in the evening primroses (Oenothera L., Onagraceae) affect quantitative genetic variation within and between populations.

• Methods: We sampled multiple genetic families from 3–5 populations from each of eight Oenothera species, which represented four independent transitions between sexual reproduction and a functionally asexual genetic system called “permanent translocation heterozygosity.” We used quantitative genetics methods to partition genetic variation within and between populations for eight plant traits related to growth, leaf physiology, flowering, and resistance to herbivores.

• Key results: Heritability was, on average, 74% higher in sexual Oenothera populations than in functionally asexual populations, with plant growth rate, specific leaf area, and the percentage of leaf water content showing the strongest differences. By contrast, genetic differentiation among populations was 2.8× higher in functionally asexual vs. sexual Oenothera species. This difference was particularly strong for specific leaf area. Sexual populations tended to exhibit higher genetic correlations among traits, but this difference was weakly supported.

• Conclusions: These results support the prediction that sexual reproduction maintains higher genetic variation within populations, which may facilitate adaptive evolution. We also found partial support for the prediction that a loss of sex leads to greater population differentiation, which may elevate speciation rates.
Complete life cycle of the lichen fungus Calopadia puiggarii (Pilocarpaceae, Ascomycetes) documented in situ: Propagule dispersal, establishment of symbiosis, thallus development, and formation of sexual and asexual reproductive structures
Authors: Sanders William B.
Published: October 2014

• Premise of the study: The life histories of lichen fungi are not well known and cannot be readily studied in laboratory culture. This work documents in situ the complete life cycle of the widespread crustose lichen Calopadia puiggarii, which reproduces sexually and asexually on the surfaces of leaves.

• Methods: Plastic cover slips held in a mesh frame were placed over leaves in the field and successively removed for microphotography of colonizing lichens.

• Key results: Macroconidia produced within campylidia encircled photobiont cells and codispersed with them, a feature not reported previously for C. puiggarii. Dispersed macroconidia readily germinated and lichenized the photobionts. Algal cells were often dislodged from the encircling macroconidia, providing a likely source for the free‐living populations observed. Aposymbiotically dispersed ascospores germinated and lichenized nearby algal cells soon after dispersal. Thallus areolae merged readily in early development, although adjacent mature thalli were often separated by growth inhibition zones. Pycnidia are reported for the first time in Calopadia; their pyriform microconidia probably function as male gametes (spermatia). Pycnidia, apothecia, and campylidia began development similarly as darkly pigmented primordia on the fungal prothallus.

• Conclusions: Abundant dispersal of ascospores, conidia, and photobionts allows C. puiggarii to quickly colonize leaves with the dual advantages of sexual and asexual reproduction, and with the added convenience of having its algal partner on hand. Fusions and prothallic capture of additional algae provide many opportunities for multiple mycobiont and photobiont genotypes to be combined in a single thallus, but the outcomes of such events remain to be explored.