bluuebunny/crossref_metadata_2025 huggingface dataset:
Conduct Problems and Hair Cortisol Concentrations Decrease in School-Aged Children after VIPP-SD: A Randomized Controlled Trial in Two Twin Cohorts
Authors: Runze Jana , Pappa Irene , Van IJzendoorn Marinus H. , Bakermans-Kranenburg Marian J.
Published: November 2022

The Video-feedback Intervention to promote Positive Parenting and Sensitive Discipline (VIPP-SD) is effective in increasing parental sensitivity and sensitive discipline, and aims to decrease child behavior problems. Changes in quality of parenting may be accompanied by effects on child stress levels. However, studies of VIPP-SD effects on child behavior problems have shown mixed results and there are no studies to date of the effect of the intervention on children’s stress levels, as measured by hair cortisol concentration (HCC). Furthermore, differences in intervention effectiveness may be explained by differential susceptibility factors. We hypothesized that the effects of the VIPP-SD on child behavior problems might be moderated by currently available child polygenic scores of differential susceptibility (PGS-DS). In the current pre-registered trial, we randomly assigned 40% of n = 445 families with school-aged twin children to the intervention group. The VIPP-SD was successful in decreasing both children’s conduct problems and HCC. Effects were not moderated by available child PGS-DS. We conclude that a brief, home-based video-feedback parenting intervention can decrease child behavior problems and affect the child’s stress-related neuroendocrine system as assessed with hair cortisol. In future studies, more specific PGS-DS for externalizing behaviors should be used as well as parental PGS-DS.
Patient-Reported Outcome Measures for Assessing Dual-Task Performance in Daily Life: A Review of Current Instruments, Use, and Measurement Properties
Authors: Abasıyanık Zuhal , Veldkamp Renee , Fostier Amber , Van Goubergen Carolien , Kalron Alon , Feys Peter
Published: November 2022

The patient perspective of dual-task (DT) impairment in real life is unclear. This review aimed (i) to identify patient-reported outcome measures (PROMs) on DT and evaluate their measurement properties and (ii) to investigate the usage of PROMs for the evaluation of DT difficulties. A systematic literature search was conducted using PubMed and Web of Science from inception to March 2022. Methodological quality was evaluated using the COSMIN checklist. Six studies examined the measurement properties of DT PROMs. Nine studies used DT PROMs as the outcome measure. Five PROMs were identified, including the Divided Attention Questionnaire (DAQ), Dual-Task-Impact on Daily-life Activities Questionnaire (DIDA-Q), a Questionnaire by Cock et al. (QOC), Dual-Tasking Questionnaire (DTQ), and Dual-Task Screening-List (DTSL). Fourteen measurement properties were documented: five (35.7%) rated quality as “sufficient”, six (42.8%) “insufficient”, and three (21.4%) “indeterminate”. The quality of evidence for each measurement property ranged from very low to high. While DT performance is investigated in many populations, the use of PROMs is still limited, although five instruments are available. Currently, due to insufficient data, it is not possible to recommend a specific DT PROM in a specific population. An exception is DIDA-Q, which has the highest quality of measurement properties in people with multiple sclerosis.
Exploring South Korean Elementary School Classroom Teachers’ Beliefs and Practices in Physical Education
Authors: Moon Jongho , Park Yongnam
Published: November 2022

Physical education (PE) is beneficial for the development of elementary school-age children through its promotion of different educational learning outcomes, which in turn affects the long-term development of physically active lifestyles. In many countries, PE is taught by classroom teachers (CTs), who are thought to be in a unique position to positively impact students’ learning. While a substantial body of studies examines the challenges that CTs encounter when teaching PE, less research has been directed towards gaining a comprehensive understanding of how CTs visualize PE and, in turn, how to promote various types of PE. The purpose of this study, therefore, was to explore elementary school CTs’ beliefs about and practices in PE in South Korea. To achieve the research goal, a semi-structured face-to face interview with six CTs was performed to collect the qualitative data source, using the ground theory as an analysis method. Three themes emerged from these interviews concerning the CTs’ beliefs in PE: (a) the importance of understanding students’ characteristics, (b) the importance of centering internal perceptions in PE method, and (c) the importance of meaningful experiences. The results also identified two themes for teaching practices in PE that corresponded to CTs’ beliefs: (a) crafting personalized instructional methods and (b) connecting PE experiences. This study lends important insights to future practices and research recommendations for CTs’ PE teaching and teacher education programs.
Home Health Care Problem with Synchronization Visits and Considering Samples Transferring Time: A Case Study in Tehran, Iran
Authors: Mirabnejad Mahyar , Mohammadi Hadi , Mirzabaghi Mehrdad , Aghsami Amir , Jolai Fariborz , Yazdani Maziar
Published: November 2022

Health care facilities have not increased in response to the growing population. Therefore, government and health agencies are constantly seeking cost-effective alternatives so they can provide effective health care to their constituents. Around the world, health care organizations provide home health care (HHC) services to patients, especially the elderly, as an efficient alternative to hospital care. In addition, recent pandemics have demonstrated the importance of home health care as a means of preventing infection. This study is the first to simultaneously take into account nurses’ working preferences and skill levels. Since transferring samples from the patient’s home to the laboratory may affect the test results, this study takes into account the time it takes to transfer samples. In order to solve large instances, two metaheuristic algorithms are proposed: Genetic Algorithms and Particle Swarm Optimization. Nurses are assigned tasks according to their time windows and the tasks’ time windows in a three-stage scheduling procedure. Using a case study set in Tehran, Iran, the proposed model is demonstrated. Even in emergencies, models can generate effective strategies. There are significant implications for health service management and health policymakers in countries where home health care services are receiving more attention. Furthermore, they contribute to the growing body of knowledge regarding health system strategies by providing new theoretical and practical insights.
The Spatiotemporal Evolution Characteristics of Cultivated Land Multifunction and Its Trade-Off/Synergy Relationship in the Two Lake Plains
Authors: Li Xigui , Xiao Pengnan , Zhou Yong , Xu Jie , Wu Qing
Published: November 2022

The material foundation of sustainable agricultural development is cultivated land resources, and their sustainable use is critical to fostering agricultural sustainability and guaranteeing national food security. In this paper, the multifunctional evaluation framework of the cultivated land system based on the “GESEL” model at the grid scale (5 km × 5 km) is constructed to explore the spatiotemporal evolution characteristics of a multifunctional cultivated land system in two lake plains and the trade-off and synergy between the functions. The five functions are all unstable in time scales, and their spatial distribution characteristics are also different. The trade-off and synergy between the multiple functions of the cultivated land system in the two lake plains from 2000 to 2019 showed significant spatial heterogeneity. Most of the functions were mainly collaborative, and a few were trade-offs. The two lake plains can be divided into four multi-functional cultivated land zones: a grain production leading zone, a distinctive agricultural planting zone, a high-efficiency agricultural development zone, and an ecological agricultural construction zone. This research puts forward some countermeasures and suggestions to promote the sustainable utilization of cultivated land resources.
Effects of Neuromuscular Electrical Stimulation and Blood Flow Restriction in Rehabilitation after Anterior Cruciate Ligament Reconstruction
Authors: Kong Doo-Hwan , Jung Won-Sang , Yang Sang-Jin , Kim Jin-Goo , Park Hun-Young , Kim Jisu
Published: November 2022

The present study aimed to examine and compare the effects of a rehabilitation exercise (RE) using neuromuscular electrical stimulation (NMES) and blood flow restriction (BFR) on muscle function and knee functional abilities in patients who underwent anterior cruciate ligament reconstruction (ACLR). A total of 45 patients who underwent ACLR (28.76 ± 0.8 years; 34 males and 11 females) were retrospectively divided into three groups: control (CON, n = 15), NMES (n = 15), and BFR (n = 15). All participants carried out the RE program for 60 min, thrice a week for 12 weeks. The Lysholm score, International Knee Documentation Committee (IKDC) subjective score, thigh circumference at 5 cm from the knee joint, Y-balance posterior medial, and lateral significantly increased in all groups via intervention (p < 0.05). However, NMES showed a higher thigh circumference at 15 cm from the knee joint than CON via intervention (p < 0.05), and the strength and endurance of quadriceps femoris and hamstrings and Y-balance anterior showed a significant increase via intervention in NMES and BFR compared with CON (p < 0.05). In conclusion, we confirmed that RE using NMES and BFR effectively enhances muscle function and balance in ACLR patients.
Extracurricular Pulse Activities in School: Students’ Attitudes and Experiences
Authors: Jägerbrink Veronica , Glaser Joakim , Östenberg Anna Hafsteinsson
Published: November 2022

Background: Few children and adolescents reach the recommended levels of daily physical activity, which is something that affects their health and wellbeing. Research shows that physical activities could be one factor for improving health and achieving academic goals in children and adolescents. Methods: Eight focus group interviews with students 10–15 years old were conducted at two schools with extracurricular pulse activities (ECPAs) during the school day. Results: In general, the interviewed students at both schools expressed positive attitudes toward ECPAs, emphasizing a felt correlation with physical activities out of school. Phenomena such as motivation, concentration and social relations also seem to profit from ECPAs. However, some students display a critical approach to ECPAs. From a gender perspective, girls embrace ECPAs with more enthusiasm than boys. Conclusions: In order to make the best use of positive attitudes and health promotion, schools need to improve structural conditions such as facilities, time pressure, unhygienic conditions, blurry boundaries between ECPAs and Physical Education (PE), uncomprehending teachers, contents and, very importantly, the inclusion of students in the process of planning and implementing ECPAs.
The Utilization of the WMO-1234 Guidance to Improve Citizen’s Wellness and Health: An Italian Perspective
Authors: Cremonini Letizia , Nardino Marianna , Georgiadis Teodoro
Published: November 2022

In 2019, the World Meteorological Organization published its “Guidance on Integrated Urban Hydrometeorological, Climate and Environment Services (Volume I: Concept and Methodology)” to assist WMO Members in developing and implementing the urban services that address the needs of city stakeholders in their countries. The guidance has relevant implications for not only protecting infrastructures from the impacts of climate change in the urban environment, but its proper declination strongly supports health-related policies to protect the population from direct and indirect impacts. Utilizing some principles of the guidance, the urbanized area of Bologna (Italy) was analyzed in order to furnish the municipality with tools coherent with the best practices actually emerging from the international bibliography to protect the citizens’ health of this city. Specifically, the analysis concentrated on the public spaces and the potential vulnerabilities of the fragile population to high-temperature regimes in the city. Utilizing the guidance as a methodological framework, the authors developed a methodology to define the microclimate vulnerabilities of the city and specific cards to assist the policymakers in city regeneration. Because the medieval structure of the city does not allow the application of a wide set of nature-based solutions, our main attention was placed on the possibility of furnishing the city with a great number of pocket parks obtainable from spaces actually dedicated to parking lots, thus introducing new green infrastructures in a highly deprived area in order to assure safety spaces for the fragile population.
Birth Weight < 3rd Percentile Prediction Using Additional Biochemical Markers—The Uric Acid Level and Angiogenesis Markers (sFlt-1, PlGF)—An Exploratory Study
Authors: Bednarek-Jędrzejek Magdalena , Dzidek Sylwia , Tousty Piotr , Kwiatkowska Ewa , Cymbaluk-Płoska Aneta , Góra Tomasz , Czuba Bartosz , Torbé Andrzej , Kwiatkowski Sebastian
Published: November 2022

(1) Aim: Ultrasound is the gold standard for assessing fetal growth disorders. The relationship between high sFlt-1/PlGF scores and LBW (low birth weight) was described. In this study, we attempted to assess whether uric acid could be used as a secondary marker in estimating the pregnancy risk associated with LBW. (2) Material and methods: 665 pregnant women with a suspected or confirmed form of placental insufficiency were enrolled. In each of the patients, sFlt-1 and PlGF and uric acid levels were determined. Patients were divided into two groups according to birth weight below and above the third percentile for the given gestational age with the criteria of the neonatal definition of FGR (fetal growth restriction). (3) Results: A significant negative correlation between neonatal birth weight and the uric acid level across the entire study group was observed. We found a significant negative correlation between neonatal birth weight and the uric acid level with birth weights < 3rd percentile. (4) Conclusions: There is a significant link between the uric acid concentration and LBW in the group with placental insufficiency. Uric acid can improve the prediction of LBW. An algorithm for LBW prognosis that makes use of biophysical (ultrasound) and biochemical (uric acid level, angiogenesis markers) parameters yields better results than using these parameters separately from each other.
Physiological Responses of a Diazotrophic Cyanobacterium to Acidification of Paddy Floodwater: N2 Fixation, Photosynthesis, and Oxidative–Antioxidative Characteristics
Authors: Yan Qiong , Xiao Peng , Li Jun , He Yaxian , Shao Jihai
Published: November 2022

Long-term of excessive fertilization using nitrogen (N) chemical fertilizer caused the acidification of paddy soils. Presently, the impacts of soil acidification on physiological characteristics of diazotrophic cyanobacteria remain unknown. In order to elucidate this issue, the effects of paddy floodwater acidification on activities of respiration, photosynthetic oxygen evolution, and N2 fixation of a paddy diazotrophic cyanobacterium Aliinostoc sp. YYLX235 were investigated in this study. In addition, the origination and quenching of intracellular reactive oxygen species (ROS) were analyzed. The acidification of paddy floodwater decreased intracellular pH and interfered in energy flux from light-harvesting chlorophyll antenna to the reaction center of photosystem II (PS II). Activities of respiration, photosynthetic oxygen evolution, and N2 fixation were decreased by the acidification of paddy floodwater. Accompanied with an increase in ROS, the level of antioxidative system increased. Superoxide dismutase (SOD) and catalase (CAT) were the main enzymatic ROS scavengers in the cells of YYLX235; reduced glutathione (GSH) was the main non-enzymatic antioxidant. Antioxidants and oxidants in the cells of YYLX235 lost balance when the pH of paddy floodwater fell to 5.0 and 4.0, and lipid oxidative damage happened. The results presented in this study suggest that the acidification of paddy soil severely interfered in the photosynthesis of diazotrophic cyanobacteria and induced the production of ROS, which in turn resulted in oxidative damage on diazotrophic cyanobacteria and a decrease in cell vitality.
Green Space Exposure and Obesity in the Mexican Adult Population
Authors: Blas-Miranda Nabetse Baruc , Lozada-Tequeanes Ana Lilia , Miranda-Zuñiga Juan Antonio , Jimenez Marcia P.
Published: November 2022

Green space or natural vegetation may reduce obesity risk by increasing opportunities for physical activity or reducing stress and exposure to other pollutants. Obesity prevalence in Mexico is ranked among the highest in the world. However, research on the association between green space and obesity in Mexico is lacking. We used data from the National Nutrition Survey in Mexico (2018–2019), a nationally representative sample of Mexican adults. The analytical sample included participants between 20–59 years of age (n = 12,631). We assessed exposure to green space using a 30 m resolution Landsat satellite Normalized Difference Vegetation Index (NDVI) from 2018. Linear regression models examined associations between NDVI and body mass index (BMI), adjusting for confounders. The mean age of the study sample was 38 (SD 0.19) years. Participants living in areas with the highest green space exposure had the lowest education level (53.51%) and socioeconomic status (28.38%) and were located in central (33.01%), south (30.37%), and rural areas (21.05%). Higher residential exposure to green space was associated with a mean decrease in BMI of −1.1 kg/m2 (95% CI: −1.59, −0.68). This is one of the first studies in Latin America to suggest a protective association between green space and obesity among Mexican adults.
Evaluation of Coastal Erosion in the Watersheds of Municipality of Buenaventura, Colombia: Using Geospatial Techniques and the Composite Vulnerability Index
Authors: Fuentes Jose Eduardo , Olaya Robin Alexis , Garcia Cesar Edwin
Published: November 2022

Buenaventura on the Colombian Pacific coast has experienced a wide range of threats, mainly due to the effects of coastal erosion and flooding. Globally, millions of people will experience increased vulnerability in the coming decades due to climate change. The change in the coastline (1986–2020) over time was analyzed with remote sensors and the Digital Shoreline Analysis System (DSAS) in conjunction with GIS. A total of 16 indicators were selected to quantitatively evaluate exposure, sensitivity, and adaptive capacity to construct a composite vulnerability index (COVI). The endpoint rate (EPR) of the change in the coastline was estimated. The results showed that 35% of the study area was stable, 18% of the coastline experienced erosion processes, and 47% experienced accretion. The COVI analysis revealed that coastal watersheds show great spatial heterogeneity; 31.4% of the area had moderate vulnerability levels, 26.5% had low vulnerability levels, and 41.9% had high vulnerability levels. This analysis revealed that the watersheds located in the northern (Málaga Bay) and central (Anchicaya, Cajambre, and Rapposo basins) parts of the coastal zone were more vulnerable than the other areas.
Advances in Clostridial and Related Neurotoxins
Authors: Pellett Sabine
Published: November 2022

The huge advances in genomics and molecular biology in the past two decades have made now an exciting time to study bacterial toxins, in particular, the most potent bacterial toxin known to humankind, botulinum neurotoxins (BoNTs) [...]
Study of Membrane-Immobilized Oxidoreductases in Wastewater Treatment for Micropollutants Removal
Authors: Zdarta Agata , Zdarta Jakub
Published: November 2022

The development of efficient strategies for wastewater treatment to remove micropollutants is of the highest importance. Hence, in this study, we presented a rapid approach to the production of biocatalytic membranes based on commercially available cellulose membrane and oxidoreductase enzymes including laccase, tyrosinase, and horseradish peroxidase. Effective enzyme deposition was confirmed based on Fourier transform infrared spectra, whereas results of spectrophotometric measurements showed that immobilization yield for all proposed systems exceeded 80% followed by over 80% activity recovery, with the highest values (over 90%) noticed for the membrane-laccase system. Further, storage stability and reusability of the immobilized enzyme were improved, reaching over 75% after, respectively, 20 days of storage, and 10 repeated biocatalytic cycles. The key stage of the study concerned the use of produced membranes for the removal of hematoporphyrin, (2,4-dichlorophenoxy)acetic acid (2,4-D), 17α-ethynylestradiol, tetracycline, tert-amyl alcohol (anesthetic drug), and ketoprofen methyl ester from real wastewater sampling at various places in the wastewater treatment plant. Although produced membranes showed mixed removal rates, all of the analyzed compounds were at least partially removed from the wastewater. Obtained data clearly showed, however, that composition of the wastewater matrix, type of pollutants as well as type of enzyme strongly affect the efficiency of enzymatic treatment of wastewater.
Peripheral mRNA Expression and Prognostic Significance of Emotional Stress Biomarkers in Metastatic Breast Cancer Patients
Authors: Fiaz Tahreem , Nadeem Muhammad Shahid , Afzal Obaid , Altamimi Abdulmalik S. A. , Alzarea Sami I. , Almalki Waleed Hassan , Khan Hafsa Ahmed , Iahtisham-Ul-Haq  , Hanook Sharoon , Kazmi Imran , Mustafa Muhammad
Published: November 2022

Emotional stress is believed to be associated with increased tumor progression. Stress-induced epigenetic modifications can contribute to the severity of disease and poor prognosis in cancer patients. The current study aimed to investigate the expression profiles along with the prognostic significance of psychological stress-related genes in metastatic breast cancer patients, to rationalize the molecular link between emotional stress and cancer progression. We profiled the expression of selected stress-associated genes (5-HTT, NR3C1, OXTR, and FKBP5) in breast cancer including the stress evaluation of all participants using the Questionnaire on Distress in Cancer Patients–short form (QSC-R10). A survival database, the Kaplan–Meier Plotter, was used to explore the prognostic significance of these genes in breast cancer. Our results showed relatively low expressions of 5-HTT (p = 0.02) and OXTR (p = 0.0387) in metastatic breast cancer patients as compared to the non-metastatic group of patients. The expression of NR3C1 was low in tumor grade III as compared to grade II (p = 0.04). Additionally, the expression of NR3C1 was significantly higher in patients with positive estrogen receptor status. However, no significant difference was found regarding FKBP5 expression in breast cancer. The results suggest a potential implication of these genes in breast cancer pathology and prognosis.
Extracellular Vesicles as Players in the Anti-Inflammatory Inter-Cellular Crosstalk Induced by Exercise Training
Authors: Catitti Giulia , De Bellis Domenico , Vespa Simone , Simeone Pasquale , Canonico Barbara , Lanuti Paola
Published: November 2022

Extracellular Vesicles (EVs) are circulating particles surrounded by a plasma membrane carrying a cargo consisting of proteins, lipids, RNAs, and DNA fragments, stemming from the cells from which they originated. EV factors (i.e., miRNAs) play relevant roles in intercellular crosstalk, both locally and systemically. As EVs increasingly gained attention as potential carriers for targeted genes, the study of EV effects on the host immune response became more relevant. It has been demonstrated that EVs regulate the host immune response, executing both pro- and anti-inflammatory functions. It is also known that physical exercise triggers anti-inflammatory effects. This review underlines the role of circulating EVs as players in the anti-inflammatory events associated with the regulation of the host’s immune response to physical exercise.
Newly Designed Primers for the Sequencing of the inlA Gene of Lineage I and II Listeria monocytogenes Isolates
Authors: Magagna Giulia , Finazzi Guido , Filipello Virginia
Published: November 2022

Listeria monocytogenes is a major human foodborne pathogen responsible for listeriosis. The virulence factor Internalin A (inlA) has a key role in the invasion of L. monocytogenes into the human intestinal epithelium, and the presence of premature stop-codons (PMSC) mutations in the inlA gene sequence is correlated with attenuated virulence. The inlA sequencing process is carried out by dividing the gene into three sections which are then reassembled to obtain the full gene. The primers available however were only able to entirely amplify the lineage II isolates. In this study, we present a set of new primers which allow inlA sequencing of isolates belonging to both lineages, since lineage I isolates are the ones most frequently associated to clinical cases. Using newly designed primers, we assessed the presence of inlA PMSCs in food, food processing environments and clinical isolates.
A Case-Control Study on the Changes in High-Sensitivity C-Reactive Protein and Tumor Necrosis Factor-Alpha Levels with Surgical Treatment of OSAS
Authors: Olszewska Ewa , Pietrewicz Tymoteusz Marek , Świderska Magdalena , Jamiołkowski Jacek , Chabowski Adrian
Published: November 2022

Obstructive sleep apnea syndrome (OSAS) is a common but underdiagnosed condition with significant health and economic implications for society. Inflammatory mediators are proposed to be associated with the presence and severity of OSAS and contribute to morbidity and mortality. This paper details a prospective non-randomized case control study of a cohort of subjects, who underwent surgical treatment of OSAS and were enrolled to assess the sleep parameters and blood levels of selected inflammatory markers at pre-operative and post-operative time points, also comparing them to the levels in a control group. A total of 25 study subjects and 18 control subjects were enrolled. Median values and interquartile range (IQR) of the apnea–hypopnea index (AHI) in the study group pre-operatively and post-operatively were 34 (18.5–45.5) and 13.3 (7.5–27.3), while in the control group 1.4 (1.0–2.1) per hour. The mean (IQR) hs-CRP levels (mg/L) were 1.782 (0.941–5.594) and 1.980 (0.990–5.445) in the study group, pre-operatively and post-operatively, respectively, while 0.891 (0.767–1.436) in the control group. The mean (IQR) TNF-α levels (pg/mL) were 7.999 (6.137–9.216) and 6.614 (5.534–7.460) pre-and post-operatively, respectively, and were 6.000 (5.026–6.823) in the control group. Results demonstrated that both inflammatory markers, hs-CRP and TNF-α, are higher in subjects with OSAS compared to the controls, and their levels decrease, but are still higher than the controls, after successful surgical treatment. Further analysis including the body mass index and age demonstrated that these changes were significant for TNF-α, but not hs-CRP.
Synthesis of a New Dinuclear Ag(I) Complex with Asymmetric Azine Type Ligand: X-ray Structure and Biological Studies
Authors: Altowyan Mezna Saleh , Soliman Saied M. , Al-Wahaib Dhuha , Barakat Assem , Ali Ali Eldissouky , Elbadawy Hemmat A.
Published: November 2022

Aspects of the molecular and supramolecular structure of the new dinuclear [Ag(L)(NO3)]2 complex, where L is 2-((E)-(((E)-1-(thiazol-2-yl)ethylidene)hydrazono)methyl)phenol, were discussed. The complex was crystallized in the monoclinic crystal system and P21/n space group. The unit cell parameters are a = 10.3274(2) Å, b = 11.4504(3) Å, c = 12.7137(3) Å and β = 108.2560(10)°. The asymmetric unit comprised one [Ag(L)(NO3)] formula in which the azine and nitrate ligand groups act as NN- and OO-bidentate chelates, respectively. The coordination environment of the Ag(I) is completed by one weak Ag-O bond with another [Ag(L)(NO3)] unit, leading to the dinuclear formula [Ag(L)(NO3)]2. This was clearly revealed by Hirshfeld analysis. Additionally, the Ag…C, O…H and C…C intermolecular interactions played an important role in the molecular packing of the studied complex. The antimicrobial, antioxidant and cytotoxic activities of the [Ag(L)(NO3)]2 complex and the free ligand (L) were discussed. While the [Ag(L)(NO3)]2 complex showed very weak antioxidant activity, the results of the antifungal and cytotoxic activities were promising. The inhibition zone diameters (IZD) and the minimum inhibitory concentration (MIC) values were determined to be 31 mm and 20 μg/mL, respectively, against A. fumigatus, which is compared to 17 mm and 156 μg/mL, respectively, for the positive control Ketoconazole. Generally, the Ag(I) complex has better antimicrobial activities than the free ligand against all microbes except for S. aureus, where the free ligand has higher activity. Additionally, the IC50 value against colon carcinoma (HCT-116 cell line) was determined to be 12.53 ± 0.69 µg/mL, which is compared to 5.35 ± 0.49 µg/mL for cis-platin. Additionally, the Ag(I) complex displays better cytotoxicity than the free ligand (L) (242.92 ± 8.12 µg/mL).
Prediction of the Current and Future Distributions of the Hessian Fly, Mayetiola destructor (Say), under Climatic Change in China
Authors: Ma Qi , Guo Jin-Long , Guo Yue , Guo Zhi , Lu Ping , Hu Xiang-Shun , Zhang Hao , Liu Tong-Xian
Published: November 2022

The Hessian fly, Mayetiola destructor (Say) (Diptera: Cecidomyiidae), is a destructive wheat pest worldwide and an important alien species in China. Based on 258 distribution records and nine environmental factors of the Hessian fly, we predicted the potential distribution area in China under three current and future (2050s and 2070s) climate change scenarios (RCP2.6, RCP4.5, and RCP8.5) via the optimized MaxEnt model. Under the current climate conditions, the suitable distribution areas of the Hessian fly in China were 25–48° N, 81–123° E, and the total highly suitable distribution area is approximately 9.63 × 105 km2, accounting for 9.99% of the total national area. The highly suitable areas are mainly located in northern Xinjiang and central and eastern China. With the rising global temperatures, except for the high-suitable areas under the RCP8.5 scenario, most potential geographic distribution areas would expand in the future. The minimum temperature in February (tmin-2), precipitation in March (prec-3), maximum temperature in November (tmax-11), and precipitation seasonality (bio-15) are important factors that affect the potential geographic distribution of the Hessian fly. This study provides an important reference and empirical basis for management of the Hessian fly in the future.
Genetic Relationships and Signatures of Adaptation to the Climatic Conditions in Populations of Apis cerana Based on the Polymorphism of the Gene Vitellogenin
Authors: Ilyasov Rustem , Rašić Slađan , Takahashi Junichi , Danilenko Valery , Proshchalykin Maxim , Lelej Arkady , Sattarov Vener , Thai Pham , Raffiudin Rika , Kwon Hyung
Published: November 2022

Apis cerana and Apis mellifera are important honey bee species in Asia. A. cerana populations are distributed from a cold, sharply continental climate in the north to a hot, subtropical climate in the south. Due to the Sacbrood virus, almost all A. cerana populations in Asia have declined significantly in recent decades and have recovered over the past five years. This could lead to a shift in the gene pool of local A. cerana populations that could affect their sustainability and adaptation. It was assumed that adaptation of honey bees could be observed by comparative analysis of the sequences of genes involved in development, labor division, and caste differentiation, such as the gene Vitellogenin VG. The VG gene nucleotide sequences were used to assess the genetic structure and signatures of adaptation of local populations of A. cerana from Korea, Russia, Japan, Nepal, and China. A. mellifera samples from India and Poland were used as the outgroup. The signatures of adaptive selection were found in the local population of A. cerana using VG gene sequence analysis based on Jukes–Cantor genetic distances, cluster analysis, dN/dS ratio evaluation, and Tajima’s D neutrality test. Based on analysis of the VG gene sequences, Apis cerana koreana subspecies in the Korean Peninsula were subdivided into three groups in accordance with their geographic localization from north to south. The VG gene sequences are acceptable tools to study the sustainability and adaptation of A. cerana populations.
Periodontal Maintenance Therapy: Efficacy of Oral Irrigator in the Home Oral Hygiene Protocol Associated with Microbiological Analysis with Phase Contrast Microscope
Authors: Caccianiga Paolo , Bader Ayt Alla , Erba Paola , Caccianiga Gianluigi
Published: November 2022

Objective: The aim of this research was to assess how the application of an oral irrigator modifies the subgingival bacterial flora in patients undergoing periodontal maintenance therapy. We used a qualitative microbiological analysis with a phase contrast microscope that can differentiate a non-pathogenic (immobile) bacterial flora from a pathogenic (mobile). Methods: In this study, 60 patients with a diagnosis of moderate periodontitis were enrolled. They were treated with non-surgical laser-assisted causal therapy and after one month a re-evaluation was performed that established the stabilization of the periodontal pathology. They were instructed in the home oral hygiene protocol with sonic toothbrush, interdental brushes and oral irrigator, and were included in the periodontal maintenance therapy program with a phase contrast microscope control every 6 months. At T0 phase contrast–phase microscopic examinations, the existence of non-pathogenic bacterial flora was displayed in all patients. They were then randomly divided into two groups, A and B. After professional oral hygiene treatment, group B suspended the usage of oral irrigator. Patients of group A, after the professional oral hygiene treatment, were motivated to continue their oral hygiene protocol at home. After three months, the patients underwent microscopic analysis of the bacterial plaque. Results: At T1 in Group B, 90% of patients had undergone a pathogenic bacterial flora change. In group A, in which patients kept using the oral irrigator for the three months, 100% of them showed immobile plaque on phase contrast microscopic analysis. Conclusions: This research showed that oral irrigator in the practice of home hygiene protocols plays a role in the long-term maintenance of a non-pathogenic bacterial flora in periodontal patients.
Medical Institutions’ Response to Child Abuse: Survey across Japan
Authors: Ishikawa Eri , Maeda Shoichi , Nakazawa Eisuke , Akabayashi Akira
Published: November 2022

The number of child abuse cases has been increasing in Japan every year. Medical institutions face issues such as coordinating with welfare offices, child guidance centers, police, and other related agencies concerning child abuse, as well as communicating with parents/guardians who are perpetrators of child abuse. This study examined the awareness and actual behavior of medical institutions regarding the support required while notifying/reporting cases of child abuse. A self-administered and anonymous questionnaire survey was conducted among the staff of the pediatrics departments, emergency departments, and general affairs departments of large hospitals in Japan. Differences in notifying and reporting child abuse cases to the child guidance center and welfare office and the police were assessed. It was found that some cases were not reported to the child guidance center or welfare office despite being considered “abuse.” Furthermore, a difference of opinion was observed among staff members, especially in judging “whether abuse is suspected or not.”
Geometry Does Impact on the Plane Strain Directions of the Human Left Ventricle, Irrespective of Disease
Authors: Piras Paolo , Colorado-Cervantes Ivan , Nardinocchi Paola , Gabriele Stefano , Varano Valerio , Esposito Giuseppe , Teresi Luciano , Torromeo Concetta , Puddu Paolo Emilio
Published: November 2022

The directions of primary strain lines of local deformation in Epicardial and Endocardial layers have been the subject of debate in recent years. Different methods led to different conclusions and a complete assessment of strain direction patterns in large and variable (in terms of pathology) cohorts of healthy and diseased patients is still lacking. Here, we use local deformation tensors in order to evaluate the angle of strain lines with respect to the horizontal circumferential direction in both Epi- and Endo-layers. We evaluated this on a large group of 193 subjects including 82 healthy control and 111 patients belonging to a great variety of pathological conditions. We found that Epicardial strain lines obliquely directed while those of Endocardium are almost circumferential. This result occurs irrespective of pathological condition. We propose that the geometric vinculum characterizing Endocardium and Epicardium in terms of different lever arm length and orientation of muscular fibers during contraction inescapably requires Endocardial strain lines to be circumferentially oriented and this is corroborated by experimental results. Further investigations on transmural structure of myocytes could couple results presented here in order to furnish additional experimental explanations.
Dynamic of Circulating DNAM-1+ Monocytes and NK Cells in Patients with STEMI Following Primary Percutaneous Coronary Intervention
Authors: Kumric Marko , Urlic Hrvoje , Bilalic Admira , Rezic-Muzinic Nikolina , Mastelic Angela , Markotic Anita , Rusic Doris , Borovac Josip A. , Duplancic Darko , Luetic Marina , Covic Ivan , Ticinovic Kurir Tina , Bozic Josko
Published: November 2022

Although the role of inflammation and adverse cardiac remodeling in myocardial infarction (MI) have been extensively explored, gaps in knowledge on the complex interaction between these processes still exist. Data suggest that DNAX accessory molecule-1 (DNAM-1), an activating receptor implicated in NK cell education, may be involved in cardiac remodeling following coronary artery occlusion. In the present study, we aimed to explore the dynamic of DNAM-1+ monocytes and NK cells in peripheral blood in the early phase following reperfusion in patients with ST-elevation MI (STEMI). The study enrolled 49 patients older than 18 years of age diagnosed with STEMI, referred to primary percutaneous coronary intervention (pPCI). Blood samples were obtained at three distinct points (at admission, 3 h, and 24 h after pPCI) and analyzed using flow cytometry. The number of circulating DNAM-1+ monocytes (CD16++ and CD14++) and CD56dimCD16++NK cells was significantly reduced 3 h after pPCI and subsequently returned to initial levels 24 h after procedure (p = 0.003, p < 0.001, and p = 0.002, respectively). Notably, such dynamic was dependent on age of patients. A positive correlation between high sensitivity troponin I levels and number of CD16++DNAM-1+ monocytes in peripheral blood 3 h after pPCI was observed (r = 0.431, p = 0.003). In conclusion, in the present study we delineated the post-reperfusion dynamic of DNAM-1-expresing leukocytes. Additionally, we demonstrated that the number of CD16++ DNAM-1+ monocytes correlate with the extent of myocardial injury.
Compressive Strength and Porosity Evaluation of Innovative Bidirectional Spiral Winding Fiber Reinforced Composites
Authors: Kharouf Naji , Sauro Salvatore , Hardan Louis , Jmal Hamdi , Bachagha Gulbahar , Macaluso Valentina , Addiego Frédéric , Inchingolo Francesco , Haikel Youssef , Mancino Davide
Published: November 2022

The aim of this in vitro study was to investigate the compressive strength and the bulk porosity of a bidirectional (bFRC) and an experimental bidirectional spiral winding reinforced fiber composite (bswFRC). Cylindrical-shape specimens were prepared for each material group and processed for the evaluation of compressive strength after different storage conditions (dry, 1 and 3 months) in distilled water at 37 °C. The specimens were also assessed for the degree of bulk porosity through X-ray tomography. A scanning electron microscope (SEM) was used to determine the fracture mode after a compressive strength test. Data were statistically analyzed using Two-Way Analysis of Variance (ANOVA). A significantly lower compressive strength was obtained in dry conditions, and after 1 month of water immersion, with the specimens created with bFRC compared to those made with bswFRC (p < 0.05). No significant difference (p > 0.05) was found between the two groups after 3 months of water immersion. However, the presence of water jeopardized significantly the compressive strength of bswFRC after water storage. The type of fracture was clearly different between the two groups; bswFRC showed a brutal fracture, whilst bFRC demonstrated a shear fracture. The bswFRC demonstrated higher pore volume density than bFRC. In conclusion, bswFRC is characterized by greater compressive strength compared to bFRC in dry conditions, but water-aging can significantly decrease the mechanical properties of such an innovative FRC. Therefore, both the novel bidirectional spiral winding reinforced fiber composites (bswFRC) and the bidirectional fiber reinforced composites (bFRC) might represent suitable materials for the production of post-and-core systems via CAD/CAM technology. These findings suggest that both FRC materials have the potential to strengthen the endodontically treated teeth.
A New Removable Helical Metallic Stent for the Treatment of Tracheomalacia in Children: Study in Pathological Animal Model
Authors: Lopez-Minguez Sandra , Rodriguez-Zapater Sergio , Bonastre Cristina , Rodriguez Jose , De Gregorio Miguel Angel , Guirola Jose Andres , Serrano-Casorran Carolina
Published: November 2022

Background: Congenital tracheomalacia is a pathology with no consensus of medical or surgical approach. The permanent nature and the major complications associated with metallic stents have limited their use over the years. The purpose of this study was to evaluate the feasibility of a helical stent design removal. Methods: Ten dogs diagnosed with tracheal collapse and treated with the helical stent were involved in the study. Animals were classified into three groups depending on stent indwelling time. Prior to the removal, endoscopic evaluation was performed to assess endothelization grade, mucous accumulation, and the presence of stenosis. During the removal, bleeding, fracture, or impossibility of removal were noted. After the removal, all macroscopic mucosal changes were recorded. Results: Technical success was 100%, without any complications. Complete epithelization of the stent was visualized in 7/10 animals. The removal procedure duration ranged from 2–12 min. At post-removal endoscopy, bleeding or epithelial damage, was visualized in any case. Stent fracture during removal occurred in one animal. Conclusions: The removal of a metallic stent with spiral geometry is feasible, simple, and without complications, regardless of the degree of neo-epithelialization.
Preclinical Evaluation of a Novel Small Molecule Inhibitor of LIM Kinases (LIMK) CEL_Amide in Philadelphia-Chromosome Positive (BCR::ABL+) Acute Lymphoblastic Leukemia (ALL)
Authors: Berrou Jeannig , Dupont Mélanie , Djamai Hanane , Adicéam Emilie , Parietti Véronique , Kaci Anna , Clappier Emmanuelle , Cayuela Jean-Michel , Baruchel André , Paublant Fabrice , Prudent Renaud , Ghysdael Jacques , Gardin Claude , Dombret Hervé , Braun Thorsten
Published: November 2022

Ph+ (BCR::ABL+) B-ALL was considered to be high risk, but recent advances in BCR::ABL-targeting TKIs has shown improved outcomes in combination with backbone chemotherapy. Nevertheless, new treatment strategies are needed, including approaches without chemotherapy for elderly patients. LIMK1/2 acts downstream from various signaling pathways, which modifies cytoskeleton dynamics via phosphorylation of cofilin. Upstream of LIMK1/2, ROCK is constitutively activated by BCR::ABL, and upon activation, ROCK leads to the phosphorylation of LIMK1/2, resulting in the inactivation of cofilin by its phosphorylation and subsequently abrogating its apoptosis-promoting activity. Here, we demonstrate the anti-leukemic effects of a novel LIMK1/2 inhibitor (LIMKi) CEL_Amide in vitro and in vivo for BCR::ABL-driven B-ALL. The IC50 value of CEL_Amide was ≤1000 nM in BCR::ABL+ TOM-1 and BV-173 cells and induced dose-dependent apoptosis and cell cycle arrest in these cell lines. LIMK1/2 were expressed in BCR::ABL+ cell lines and patient cells and LIMKi treatment decreased LIMK1 protein expression, whereas LIMK2 expression was unaffected. As expected, CEL_Amide exposure caused specific activating downstream dephosphorylation of cofilin in cell lines and primary cells. Combination experiments with CEL_Amide and BCR::ABL TKIs imatinib, dasatinib, nilotinib, and ponatinib were synergistic for the treatment of both TOM-1 and BV-173 cells. CDKN2Ako/BCR::ABL1+ B-ALL cells were transplanted in mice, which were treated with combinations of CEL_Amide and nilotinib or ponatinib, which significantly prolonged their survival. Altogether, the LIMKi CEL_Amide yields activity in Ph+ ALL models when combined with BCR::ABL-targeting TKIs, showing promising synergy that warrants further investigation.
Physicians and Machine-Learning Algorithm Performance in Predicting Left-Ventricular Systolic Dysfunction from a Standard 12-Lead-Electrocardiogram
Authors: Golany Tomer , Radinsky Kira , Kofman Natalia , Litovchik Ilya , Young Revital , Monayer Antoinette , Love Itamar , Tziporin Faina , Minha Ido , Yehuda Yakir , Ziv-Baran Tomer , Fuchs Shmuel , Minha Sa’ar
Published: November 2022

Early detection of left ventricular systolic dysfunction (LVSD) may prompt early care and improve outcomes for asymptomatic patients. Standard 12-lead ECG may be used to predict LVSD. We aimed to compare the performance of Machine Learning Algorithms (MLA) and physicians in predicting LVSD from a standard 12-lead ECG. By utilizing a dataset of 13,820 pairs of ECGs and echocardiography, a deep residual convolutional neural network was trained for predicting LVSD (ejection fraction (EF) < 50%) from ECG. The ECGs of the test set (n = 850) were assessed for LVSD by the MLA and six physicians. The performance was compared using sensitivity, specificity, and C-statistics. The interobserver agreement between the physicians for the prediction of LVSD was moderate (κ = 0.50), with average sensitivity and specificity of 70%. The C-statistic of the MLA was 0.85. Repeating this analysis with LVSD defined as EF < 35% resulted in an improvement in physicians’ average sensitivity to 84% but their specificity decreased to 57%. The MLA C-statistic was 0.88 with this threshold. We conclude that although MLA outperformed physicians in predicting LVSD from standard ECG, prior to robust implementation of MLA in ECG machines, physicians should be encouraged to use this approach as a simple and readily available aid for LVSD screening.