bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Enniatin B and Beauvericin on Lysosomal Cathepsin B Secretion and Apoptosis Induction
Authors: Aufy Mohammed , Abdelaziz Ramadan F. , Hussein Ahmed M. , Topcagic Nermina , Shamroukh Hadil , Abdel-Maksoud Mostafa A. , Salem Tamer Z. , Studenik Christian R.
Published: January 2023

Enniatin B (ENN B) and Beauvericin (BEA) are cyclohexadepsipeptides that can be isolated from Fusarium and Beauveria bassiana, respectively. Both compounds are cytotoxic and ionophoric. In the present study, the mechanism of cell death induced by these compounds was investigated. Epidermal carcinoma-derived cell line KB-3-1 cells were treated with different concentrations of these compounds. The extracellular secretion of cathepsin B increased in a concentration-dependent manner, and the lysosomal staining by lysotracker red was reduced upon the treatment with any of the compounds. However, the extracellular secretion of cathepsin L and cathepsin D were not affected. Inhibition of cathepsin B with specific inhibitor CA074 significantly reduced the cytotoxic effect of both compounds, while inhibition of cathepsin D or cathepsin L did not influence the cytotoxic activities of both compounds. In vitro labelling of lysosomal cysteine cathepsins with Ethyl (2S, 3S)-epoxysuccinate-Leu-Tyr-Acp-Lys (Biotin)-NH2 (DCG04) was not affected in case of cathepsin L upon the treatment with both compounds, while it was significantly reduced in case of cathepsin B. In conclusion, ENN B and BEA increase lysosomal Ph, which inhibits delivery of cathepsin B from Golgi to lysosomes, thereby inducing cathepsin B release in cytosol, which activates caspases and hence the apoptotic pathway.
Sympathetic System in Wound Healing: Multistage Control in Normal and Diabetic Skin
Authors: Ivanov Evgenii , Akhmetshina Marina , Erdiakov Aleksei , Gavrilova Svetlana
Published: January 2023

In this review, we discuss sympathetic regulation in normal and diabetic wound healing. Experimental denervation studies have confirmed that sympathetic nerve endings in skin have an important and complex role in wound healing. Vasoconstrictor neurons secrete norepinephrine (NE) and neuropeptide Y (NPY). Both mediators decrease blood flow and interact with inflammatory cells and keratinocytes. NE acts in an ambiguous way depending on receptor type. Beta2-adrenoceptors could be activated near sympathetic endings; they suppress inflammation and re-epithelialization. Alpha1- and alpha2-adrenoceptors induce inflammation and activate keratinocytes. Sudomotor neurons secrete acetylcholine (ACh) and vasoactive intestinal peptide (VIP). Both induce vasodilatation, angiogenesis, inflammation, keratinocytes proliferation and migration. In healthy skin, all effects are important for successful healing. In treatment of diabetic ulcers, mediator balance could be shifted in different ways. Beta2-adrenoceptors blockade and nicotinic ACh receptors activation are the most promising directions in treatment of diabetic ulcers with neuropathy, but they require further research.
Dietary Strawberries Improve Serum Metabolites of Cardiometabolic Risks in Adults with Features of the Metabolic Syndrome in a Randomized Controlled Crossover Trial
Authors: Basu Arpita , Izuora Kenneth , Hooyman Andrew , Scofield Hal R. , Ebersole Jeffrey L.
Published: January 2023

Dietary strawberries have been shown to improve cardiometabolic risks in multiple clinical trials. However, no studies have reported effects on serum metabolomic profiles that may identify the target pathways affected by strawberries as underlying mechanisms. We conducted a 14-week randomized, controlled crossover study in which participants with features of metabolic syndrome were assigned to one of the three arms for four weeks separated by a one-week washout period: control powder, 1 serving (low dose: 13 g strawberry powder/day), or 2.5 servings (high dose: 32 g strawberry powder/day). Blood samples, anthropometric measures, blood pressure, and dietary and physical activity data were collected at baseline and at the end of each four-week phase of intervention. Serum samples were analyzed for primary metabolites and complex lipids using different mass spectrometry methods. Mixed-model ANOVA was used to examine differences in the targeted metabolites between treatment phases, and LASSO logistic regression was used to examine differences in the untargeted metabolites at end of the strawberry intervention vs. the baseline. The findings revealed significant differences in the serum branched-chain amino acids valine and leucine following strawberry intervention (high dose) compared with the low-dose and control phases. Untargeted metabolomic profiles revealed several metabolites, including serum phosphate, benzoic acid, and hydroxyphenyl propionic acid, that represented improved energy-metabolism pathways, compliance measures, and microbial metabolism of strawberry polyphenols, respectively. Thus, dietary supplementation of strawberries significantly improves the serum metabolic profiles of cardiometabolic risks in adults.
Neurotoxic/Neuroprotective Effects of Clozapine and the Positive Allosteric Modulator of mGluR2 JNJ-46356479 in Human Neuroblastoma Cell Cultures
Authors: Gassó Patricia , Martínez-Pinteño Albert , Rodríguez Natalia , Madero Santiago , Gómez Marta , Segura Alex G. , García-Rizo Clemente , Morén Constanza , Mas Sergi , Parellada Eduard
Published: January 2023

Current antipsychotics (APs) effectively control positive psychotic symptoms, mainly by blocking dopamine (DA) D2 receptors, but have little effect on negative and cognitive symptoms. Increased glutamate (GLU) release would trigger neurotoxicity, leading to apoptosis and synaptic pruning, which is involved in the pathophysiology of schizophrenia. New pharmacological strategies are being developed such as positive allosteric modulators (PAMs) of the metabotropic GLU receptor 2 (mGluR2) that inhibit the presynaptic release of GLU. We previously reported that treatment of adult mice with JNJ-46356479 (JNJ), a recently developed mGluR2 PAM, partially improved neuropathological deficits and schizophrenia-like behavior in a postnatal ketamine mouse model. In the present study, we evaluated, for the first time, the putative neuroprotective and antiapoptotic activity of JNJ in a human neuroblastoma cell line and compared it with the effect of clozapine (CLZ) as a clinical AP with the highest efficacy and with apparent utility in managing negative symptoms. Specifically, we measured changes in cell viability, caspase 3 activity and apoptosis, as well as in the expression of key genes involved in survival and cell death, produced by CLZ and JNJ alone and in combination with a high DA or GLU concentration as apoptosis inducers. Our results suggest that JNJ is not neurotoxic and attenuates apoptosis, particularly by decreasing the caspase 3 activation induced by DA and GLU, as well as increasing and decreasing the number of viable and apoptotic cells, respectively, only when cultures were exposed to GLU. Its effects seem to be less neurotoxic and more neuroprotective than those observed with CLZ. Moreover, JNJ partially normalized altered expression levels of glycolytic genes, which could act as a protective factor and be related to its putative neuroprotective effect. More studies are needed to define the mechanisms of action of this GLU modulator and its potential to become a novel therapeutic agent for schizophrenia.
In Silico Simulation of Impacts of Metal Nano-Oxides on Cell Viability in THP-1 Cells Based on the Correlation Weights of the Fragments of Molecular Structures and Codes of Experimental Conditions Represented by Means of Quasi-SMILES
Authors: Toropova Alla P. , Toropov Andrey A. , Fjodorova Natalja
Published: January 2023

A simulation of the effect of metal nano-oxides at various concentrations (25, 50, 100, and 200 milligrams per millilitre) on cell viability in THP-1 cells (%) based on data on the molecular structure of the oxide and its concentration is proposed. We used a simplified molecular input-line entry system (SMILES) to represent the molecular structure. So-called quasi-SMILES extends usual SMILES with special codes for experimental conditions (concentration). The approach based on building up models using quasi-SMILES is self-consistent, i.e., the predictive potential of the model group obtained by random splits into training and validation sets is stable. The Monte Carlo method was used as a basis for building up the above groups of models. The CORAL software was applied to building the Monte Carlo calculations. The average determination coefficient for the five different validation sets was R2 = 0.806 ± 0.061.
Influence of Light of Different Spectral Compositions on Growth Parameters, Photosynthetic Pigment Contents and Gene Expression in Scots Pine Plantlets
Authors: Pashkovskiy Pavel , Ivanov Yury , Ivanova Alexandra , Kreslavski Vladimir D. , Vereshchagin Mikhail , Tatarkina Polina , Kuznetsov Vladimir V. , Allakhverdiev Suleyman I.
Published: January 2023

The photoreceptors of red light (phytochromes) and blue light (cryptochromes) impact plant growth and metabolism. However, their action has been barely studied, especially in coniferous plants. Therefore, the influence of blue (maximum 450 nm), red (maximum 660 nm), white light (maxima 450 nm + 575 nm), far-red light (maximum 730 nm), white fluorescent light and dark on seed germination, growth, chlorophyll and carotenoid contents, as well as the transcript levels of genes involved in reception, photosynthesis, light and hormonal signaling of Scots pine plantlets, was investigated. The highest values of dry weight, root length and photosynthetic pigment contents were characteristic of 9-day-old plantlets grown under red light, whereas in the dark plantlet length, seed vigor, seed germination, dry weight and pigment contents were decreased. Under blue and white lights, the main studied morphological parameters were decreased or close to red light. The cotyledons were undeveloped under dark conditions, likely due to the reduced content of photosynthetic pigments, which agrees with the low transcript levels of genes encoding protochlorophyllide oxidoreductase (PORA) and phytoene synthase (PSY). The transcript levels of a number of genes involved in phytohormone biosynthesis and signaling, such as GA3ox, RRa, KAO and JazA, were enhanced under red light, unlike under dark conditions. We suggest that the observed phenomena of red light are the most important for the germination of the plantlets and may be based on earlier and enhanced expression of auxin, cytokinin, gibberellin and jasmonate signaling genes activated by corresponding photoreceptors. The obtained results may help to improve reforestation technology; however, this problem needs further study.
New Role of Water in Transketolase Catalysis
Authors: Solovjeva Olga N.
Published: January 2023

Transketolase catalyzes the interconversion of keto and aldo sugars. Its coenzyme is thiamine diphosphate. The binding of keto sugar with thiamine diphosphate is possible only after C2 deprotonation of its thiazole ring. It is believed that deprotonation occurs due to the direct transfer of a proton to the amino group of its aminopyrimidine ring. Using mass spectrometry, it is shown that a water molecule is directly involved in the deprotonation process. After the binding of thiamine diphosphate with transketolase and its subsequent cleavage, a thiamine diphosphate molecule is formed with a mass increased by one oxygen molecule. After fragmentation, a thiamine diphosphate molecule is formed with a mass reduced by one and two hydrogen atoms, that is, HO and H2O are split off. Based on these data, it is assumed that after the formation of holotransketolase, water is covalently bound to thiamine diphosphate, and carbanion is formed as a result of its elimination. This may be a common mechanism for other thiamine enzymes. The participation of a water molecule in the catalysis of the one-substrate transketolase reaction and a possible reason for the effect of the acceptor substrate on the affinity of the donor substrate for active sites are also shown.
Monomeric C-Reactive Protein in Atherosclerotic Cardiovascular Disease: Advances and Perspectives
Authors: Melnikov Ivan , Kozlov Sergey , Saburova Olga , Avtaeva Yuliya , Guria Konstantin , Gabbasov Zufar
Published: January 2023

This review aimed to trace the inflammatory pathway from the NLRP3 inflammasome to monomeric C-reactive protein (mCRP) in atherosclerotic cardiovascular disease. CRP is the final product of the interleukin (IL)-1β/IL-6/CRP axis. Its monomeric form can be produced at sites of local inflammation through the dissociation of pentameric CRP and, to some extent, local synthesis. mCRP has a distinct proinflammatory profile. In vitro and animal-model studies have suggested a role for mCRP in: platelet activation, adhesion, and aggregation; endothelial activation; leukocyte recruitment and polarization; foam-cell formation; and neovascularization. mCRP has been shown to deposit in atherosclerotic plaques and damaged tissues. In recent years, the first published papers have reported the development and application of mCRP assays. Principally, these studies demonstrated the feasibility of measuring mCRP levels. With recent advances in detection techniques and the introduction of first assays, mCRP-level measurement should become more accessible and widely used. To date, anti-inflammatory therapy in atherosclerosis has targeted the NLRP3 inflammasome and upstream links of the IL-1β/IL-6/CRP axis. Large clinical trials have provided sufficient evidence to support this strategy. However, few compounds target CRP. Studies on these agents are limited to animal models or small clinical trials.
Subchondral Bone Cyst Development in Osteoarthritis: From Pathophysiology to Bone Microarchitecture Changes and Clinical Implementations
Authors: Kaspiris Angelos , Hadjimichael Argyris C. , Lianou Ioanna , Iliopoulos Ilias D. , Ntourantonis Dimitrios , Melissaridou Dimitra , Savvidou Olga D. , Papadimitriou Evangelia , Chronopoulos Efstathios
Published: January 2023

Osteoarthritis is a degenerative joint disease affecting middle-aged and elderly patients. It mainly involves weight-bearing joints such as the hip, knee and spine as well as the basilar joint of the thumb, causing dysfunction and painful symptoms. Often, joint arthritis is accompanied by cartilage defects, joint space narrowing, osteophytes, bone sclerosis and subchondral bone cysts (SBC). The aim of the present study was to explore the pathophysiology responsible for the development of SBCs as well as the association between SBCs and disease progress, the level of clinical symptoms and their impact on postoperative outcomes and risk of possible complications following joint replacements if left untreated. A literature review on PubMed articles was conducted to retrieve and evaluate all available evidence related to the main objective mentioned above. A few theories have been put forth to explain the formation process of SBCs. These involve MMPs secretion, angiogenesis, and enhanced bone turnover as a biological response to abnormal mechanical loads causing repeated injuries on cartilage and subchondral tissue during the development of arthritis. However, the application of novel therapeutics, celecoxib-coated microspheres, local administration of IGF-1 and activated chondrocytes following surgical debridement of SBCs hinders the expansion of SBCs and prevents the progression of osteoarthritis.
Quantifying the Influence of Factors on the Accuracy of Speech Perception in Mandarin-Speaking Cochlear Implant Patients
Authors: Mao Xiang , Zhang Ziyue , Chen Yu , Wang Yue , Yang Yijing , Wei Mei , Liu Yao , Ma Yuanxu , Lin Peng , Wang Wei
Published: January 2023

Rehabilitation of hearing perception in cochlear implant (CI) patients is a challenging process. A comprehensive analysis of the characteristics of hearing rehabilitation in Mandarin-speaking CI patients was conducted. We measured the aided hearing threshold (AHT) and the speech perception accuracy (SPA) and collected clinical data. A total of 49 CI patients were included. Significant linear relationships existed between the AHT and SPA. The SPA increased by about 5–7% when the AHT decreased by 5 dB. An apparent individual difference in the SPA was observed under the same AHT, which in some patients was lower than the reference value fitted by the regression model. The timing of both of cochlear implantation and rehabilitation training was found to lead to significant improvement in SPA. The SPA increases by 2.1–3.6% per year of cochlear implantation and 0.7–1.5% per year of rehabilitation training. The time of auditory deprivation can significantly reduce the SPA by about 1.0–1.6% per year. The SPA was still poor in some CI patients when the hearing compensation seemed satisfying. Early cochlear implantation and post-operative rehabilitation are essential for recovery of the patient’s SPA if the indications for cochlear implantation are met.
Does Age Influence the Preoperative Condition and, Thus, the Outcome in Endocarditis Patients?
Authors: Ostovar Roya , Zinab Farzaneh Seifi , Schröter Filip , Hartrumpf Martin , Fritzsche Dirk , Albes Johannes Maximilian
Published: January 2023

Background: Demographic changes have led to an increase in the proportion of older patients undergoing heart surgery. The number of endocarditis cases is also steadily increasing. Given the sharp increase in patients who have received valve prostheses or electrophysiological implants, who are on chronic dialysis or taking immunosuppressants, the interdependence of these two developments is quite obvious. We have studied the situation of older patients suffering from endocarditis compared to younger ones. Are they more susceptible, and are there differences in outcomes? Patients and Methods: A total of 162 patients was studied from our database, enrolled from 2020 to 2022. Fifty-four of them were older than 75 years of age (mean age 79.9 ± 3.8 years). The remaining 108 patients had a mean age of 61.6 ± 10.1 years. EuroSCORE II (ES II) was higher in the older patients (19.3 ± 19.7) than in the younger ones (13.2 ± 16.84). The BMI was almost identical. The preoperative NYHA proportions did not differ. A statistical analysis was performed using R. Results: Older patients had a lower left ventricular ejection fraction (LVEF), a higher proportion of coronary heart disease (CHD), a higher amount of N-terminal probrain natriuretic peptides (NT-proBNPs), worse coagulation function, worse renal function than younger patients, and were more often valve prosthesis carriers compared to the younger patients. The diagnostic interval was 66.85 ± 49.53 days in the younger cohort, whereas it was only 50.98 ± 30.55 in the elderly (p = 0.081). Significantly fewer septic emboli were observed in the older patients than in the younger patients, but postoperative delirium and critical illness polyneuropathy and critical illness myopathy (CIP/CIM) were observed significantly more frequently compared to younger patients. In-hospital mortality was higher in older patients than in younger patients, but did not reach statistical significance (29.91% vs. 40.38%; p = 0.256). The postoperative clinical status was worse in older patients than in the younger ones (NYHA-stage, p = 0.022). Conclusions: Age did have an impact on the outcome, probably due to causing a higher number of cumulative preoperative risk factors. However, an interesting phenomenon was that older patients had fewer septic emboli than younger patients. It can only be speculated whether this was due to a shorter diagnostic interval or lower mobility, i.e., physical exertion. Older patients suffered more frequently than younger ones from typical age-related postoperative complications, such as delirium and CIP/CIM. In-hospital mortality was high, but not significantly higher compared to the younger patients. Considering the acceptable mortality risks, and in light of the lack of alternatives, older patients should not be denied surgery. However, individual consideration is necessary.
Frontal Fibrosing Alopecia Quality of Life Index: A Validated Disease-Specific Questionnaire Involving Women
Authors: Porriño-Bustamante María Librada , Montero-Vílchez Trinidad , Fernández-Pugnaire María Antonia , Arias-Santiago Salvador
Published: January 2023

Quality of life (QoL) can be affected in patients with alopecia. The few studies that evaluate QoL in FFA use unspecific questionnaires. The aim of this report was to design and validate a specific questionnaire to assess the impairment of QoL in FFA patients. A specific questionnaire, called the Frontal Fibrosing Alopecia Quality of Life Index (FFA-QLI), was designed and validated using the Dermatology Life Quality Index (DLQI). One-hundred and one women with FFA were included. Cronbach’s alpha value was 0.865, and the intraclass correlation coefficient between all the items in the questionnaire was 0.870. The FFA-QLI correlated positively with the DLQI (r = 0.729, p < 0.001). Patients with severe FFA showed a higher FFA-QLI (19.72) score compared to those with a mild disease (14.11) (p = 0.002), and the area under the curve for identifying severe disease was greater in the FFA-QLI than in the DLQI. The cut-off points were used to select patients with mild, moderate, and severe impairment in QoL. A score < 21 in the FFA-QLI corresponded to a low impact on QoL; values > 35 matched with greater QoL impairment; and values ranging from 21 to 35 corresponded to moderate QoL alteration. To conclude, a validated disease-specific questionnaire to assess QoL in FFA patients is here presented, with a greater power to discriminate severe cases of FFA than the DLQI.
Correction: Burgos-Mansilla et al. Effect of Physical Therapy Modalities on Quality of Life of Head and Neck Cancer Survivors: A Systematic Review with Meta-Analysis. J. Clin. Med. 2021, 10, 4696
Authors: Burgos-Mansilla Barbara , Galiano-Castillo Noelia , Lozano-Lozano Mario , Fernández-Lao Carolina , Lopez-Garzon Maria , Arroyo-Morales Manuel
Published: January 2023

In the original publication [...]
Correlations between Maternal and Fetal Outcomes in Pregnant Women with Kidney Failure
Authors: Meca Daniela-Catalina , Varlas Valentin Nicolae , Mehedințu Claudia , Cîrstoiu Monica Mihaela
Published: January 2023

Background: Kidney function impairment in pregnancy is challenging, with incidence and prognosis only partially known. Studies concerning maternal and fetal outcomes in pregnancies occurring in patients with renal injury and the therapeutic strategies for improving the prognosis of these patients are scarce due to the limited number of cases reported. Objectives: We aimed to establish correlations between the main maternal and fetal outcomes in patients with severe CKD or AKI in pregnancy to improve the prognosis, referring to a control group of patients with mild kidney impairment. Methods: For this purpose, we conducted a retrospective study, at University Emergency Hospital in Bucharest, Romania, from January 2019 until December 2021, selecting 38 patients with AKI and 12 patients diagnosed with advanced CKD, compared to 42 patients displaying borderline values of serum creatinine (0.8–1 mg/dL), reflecting the presence of milder kidney impairment. Results: The probability of having a child that is premature and small for gestational age, with a lower Apgar score and more frequent neonatal intensive care unit admissions, delivered by cesarean section, is higher in patients with highly reduced kidney function. Conclusion: Severe kidney function impairment is associated with a grim fetal prognosis and obstetrical complications.
Assessment and Management of Frailty
Authors: Kato Yuki , Shimizu Akio , Momosaki Ryo
Published: January 2023

Frailty has become a major problem for an increasing number of older people worldwide [...]
Preparation and Characterization of Patch Loaded with Clarithromycin Nanovesicles for Transdermal Drug Delivery
Authors: Zaid Alkilani Ahlam , Musleh Batool , Hamed Rania , Swellmeen Lubna , Basheer Haneen A.
Published: January 2023

Clarithromycin (CLR), categorized as a Biopharmaceutical Classification System class II drug, has several gastrointestinal tract side effects and an extremely unpalatable bitter taste. The current study aimed to design transdermal patch-embedded CLR niosomes to overcome the aforementioned CLR-related challenges. Various niosomal formulations were successfully fabricated and characterized for their morphology, size, in vitro release, and antimicrobial efficacy. Subsequently, the CLR niosomes were loaded into transdermal patches using the solvent casting method. The polydispersity index of the niosomes ranged from 0.005 to 0.360, indicating the uniformity of the niosomes. The encapsulating efficiency (EE)% varied from 12 to 86%. The optimal Chol: surfactant ratio for drug release was found to be 0.5:1. In addition, the encapsulation of CLR into niosomal nanovesicles did not reduce the antibacterial activity of the CLR. The niosomal patch had a significantly higher permeability coefficient of CLR than the conventional patch. In addition to that, a shear-thinning behavior was observed in the niosomal gels before loading them into a niosomal patch. The flux (Jss) of the niosomal patch was significantly higher than the conventional patch by more than 200 times. In conclusion, niosome-based transdermal patches could be a promising method for the transdermal drug delivery of class II drugs and drugs experiencing GIT side effects.
Path Tracing vs. Volume Rendering Technique in Post-Surgical Assessment of Bone Flap in Oncologic Head and Neck Reconstructive Surgery: A Preliminary Study
Authors: Cardobi Nicolò , Nocini Riccardo , Molteni Gabriele , Favero Vittorio , Fior Andrea , Marchioni Daniele , Montemezzi Stefania , D’Onofrio Mirko
Published: January 2023

This study aims to compare a relatively novel three-dimensional rendering called Path Tracing (PT) to the Volume Rendering technique (VR) in the post-surgical assessment of head and neck oncologic surgery followed by bone flap reconstruction. This retrospective study included 39 oncologic patients who underwent head and neck surgery with free bone flap reconstructions. All exams were acquired using a 64 Multi-Detector CT (MDCT). PT and VR images were created on a dedicated workstation. Five readers, with different expertise in bone flap reconstructive surgery, independently reviewed the images (two radiologists, one head and neck surgeon and two otorhinolaryngologists, respectively). Every observer evaluated the images according to a 5-point Likert scale. The parameters assessed were image quality, anatomical accuracy, bone flap evaluation, and metal artefact. Mean and median values for all the parameters across the observer were calculated. The scores of both reconstruction methods were compared using a Wilcoxon matched-pairs signed rank test. Inter-reader agreement was calculated using Spearman’s rank correlation coefficient. PT was considered significantly superior to VR 3D reconstructions by all readers (p < 0.05). Inter-reader agreement was moderate to strong across four out of five readers. The agreement was stronger with PT images compared to VR images. In conclusion, PT reconstructions are significantly better than VR ones. Although they did not modify patient outcomes, they may improve the post-surgical evaluation of bone-free flap reconstructions following major head and neck surgery.
Regional and Remote Influence on the Sea Ice in the Kara Sea
Authors: Prokhorova Uliana , Alekseev Genrikh , Vyazilova Anastasia
Published: January 2023

This article examines the relationship between interannual changes in the sea ice extent and thickness in the Kara Sea with climate change in the region and with sea surface temperature in the tropical North Atlantic. The data from observations at meteorological stations, ERA5 reanalysis, and data on the sea ice from the AARI website for 1979–2021 were used. The growth of ice in winter is most influenced by air temperature and downward long-wave radiation. In summer, interannual changes in sea ice extent are closely related to air temperature. The remote influence of the sea surface temperature anomalies in the tropics of the North Atlantic on the summer (July–September) sea ice in the Kara Sea is discovered 33–35 months later. A significant correlation between climate and sea ice anomalies can serve as the basis for predicting up to four years ahead.
Multi-Objective Genetic Algorithm for Cluster Analysis of Single-Cell Transcriptomes
Authors: Zhao Konghao , Grayson Jason M. , Khuri Natalia
Published: January 2023

Cells are the basic building blocks of human organisms, and the identification of their types and states in transcriptomic data is an important and challenging task. Many of the existing approaches to cell-type prediction are based on clustering methods that optimize only one criterion. In this paper, a multi-objective Genetic Algorithm for cluster analysis is proposed, implemented, and systematically validated on 48 experimental and 60 synthetic datasets. The results demonstrate that the performance and the accuracy of the proposed algorithm are reproducible, stable, and better than those of single-objective clustering methods. Computational run times of multi-objective clustering of large datasets were studied and used in supervised machine learning to accurately predict the execution times of clustering of new single-cell transcriptomes.
Modelling Impact of Urban Expansion on Ecosystem Services: A Scenario-Based Approach in a Mixed Natural/Urbanised Landscape
Authors: Mohammadyari Fatemeh , Zarandian Ardavan , Mirsanjari Mir Mehrdad , Suziedelyte Visockiene Jurate , Tumeliene Egle
Published: January 2023

The present study aims at predicting future land use/land cover (LULC) and quantifying and mapping the ecosystem services (ESs) of water yield, outdoor recreation opportunity and food production in current (here, 2017) and future landscapes in Northern Iran, using the InVEST, Recreation Opportunity Spectrum (ROS) and yield models. To that end, two LULC scenarios known as business as usual (BAU) and protection-based (PB) plan were applied for 2028, using the Markov Artificial Neural Network and Multi-objective land allocation (MOLA) models. The results show that rapid urbanisation, caused by the expansion of human settlements and industrial areas, has led to a decline in the ESs in the region. Compared to the ESs in 2017, the service of water yield increases as urban expansion increases, whereas food production and recreation services decrease as urban expansion increases, under the BAU scenario. On the other hand, in the PB scenario, relatively better conditions can be observed for all three ESs. Considering that the ecological structures of this region have been severely affected by rapid urban expansion, the results of this research will be useful for maintaining the existing ESs and can greatly affect planning and decision-making regarding future development towards urban sustainability.
Impacts of Aging Agricultural Labor Force on Land Transfer: An Empirical Analysis Based on the China Family Panel Studies
Authors: Li Chaozhu , Li Xiaoliang , Wang Jiaxu , Feng Tianchu
Published: January 2023

Aging is an important trend in the global demographic, with rural population aging becoming a significant challenge due to its faster pace and profound implications. Although the most significant impact of the aging agricultural labor force occurs in agricultural production and land use, little is known about their relationship. Based on the 2010–2018 data from the Chinese Family Panel Studies, this study uses the panel probit model to analyze the impact of the aging agricultural labor force on land transfer and tests its influence mechanism from the aspects of health effect and pension insurance effect. The results show the following: (1) there is an inverted U-shaped relationship between the aging of the agricultural labor force and land rent-in—with the deepening of the aging of the agricultural labor force, the aging of the agricultural labor force shifts from promoting land rent-in to inhibiting it; (2) there is a U-shaped relationship between the aging of the agricultural labor force and land rent-out—with the deepening of the aging of the agricultural labor force, the aging of the agricultural labor force shifts from inhibiting land rent-out to promoting it; and (3) the impact of the aging agricultural labor force on land rent-in and rent-out is significantly affected by farmers’ health levels, however pension insurance does not play a significant moderating role. Therefore, the government should strengthen the land transfer market and improve the level of pension insurance for the elderly rural agricultural labor force.
Sanded Wheel–Rail Contacts: Experiments on Sand Crushing Behaviour
Authors: Suhr Bettina , Skipper William A. , Lewis Roger , Six Klaus
Published: January 2023

In railway operation, the sanding process is used to overcome low adhesion conditions in the wheel–rail contact. In the literature, previously conducted research has been experimental, e.g., measuring adhesion coefficients (ACs) under different contact conditions (dry, wet, …) or applying different sands. Under dry conditions, sanding can reduce measured ACs, while under wet conditions different types of rail sand can leave ACs unchanged or increase adhesion. Despite active research, the physical mechanisms causing the change in ACs under sanded conditions are still poorly understood. A possible remedy is the development of advanced models of sanding including local effects. As a basis for such a model, this study presents experimental results concerning single grain crushing behaviour of two types of rail sand under dry and wet contact conditions. Firstly, initial breakage behaviour is investigated with focus on the particle fragments’ size and spread as only fragments within the running band are available to influence the AC during roll-over. Secondly, single grain crushing tests are conducted under realistic wheel–rail load showing the formation of solidified clusters of sand fragments, as well as their size and thickness. This information is important for understanding mechanisms and for future physics-based modelling of the sanding process in wheel–rail contacts.
Solution-Processed OLED Based on a Mixed-Ligand Europium Complex
Authors: Kozlov Makarii I. , Kuznetsov Kirill M. , Goloveshkin Alexander S. , Burlakin Andrei , Sandzhieva Maria , Makarov Sergey V. , Ilina Elena , Utochnikova Valentina V.
Published: January 2023

An approach to increase the efficiency of europium-based OLEDs was proposed through the formation of a mixed-ligand complex. The design of a series of europium complexes, together with an optimization of the solution deposition, including the host selection, as well as the variation of the solvent and deposition parameters, resulted in a noticeable increase in OLED luminance. As a result, the maximum luminance of the Eu-based OLED reached up to 700 cd/m2, which is one of the highest values for an Eu-based solution-processed OLED. Finally, its stability was investigated.
Effect of Hydrogen Peroxide on the Surface and Attractiveness of Various Zirconia Implant Materials on Human Osteoblasts: An In Vitro Study
Authors: Tuna Taskin , Wein Martin , Altmann Brigitte , Steinberg Thorsten , Fischer Jens , Att Wael
Published: January 2023

The aim of this in vitro study was to investigate the effect of hydrogen peroxide (H2O2) on the surface properties of various zirconia-based dental implant materials and the response of human alveolar bone osteoblasts. For this purpose, discs of two zirconia-based materials with smooth and roughened surfaces were immersed in 20% H2O2 for two hours. Scanning electron and atomic force microscopy showed no topographic changes after H2O2-treatment. Contact angle measurements (1), X-ray photoelectron spectroscopy (2) and X-ray diffraction (3) indicated that H2O2-treated surfaces (1) increased in hydrophilicity (p < 0.05) and (2) on three surfaces the carbon content decreased (33–60%), while (3) the monoclinic phase increased on all surfaces. Immunofluorescence analysis of the cell area and DNA-quantification and alkaline phosphatase activity revealed no effect of H2O2-treatment on cell behavior. Proliferation activity was significantly higher on three of the four untreated surfaces, especially on the smooth surfaces (p < 0.05). Within the limitations of this study, it can be concluded that exposure of zirconia surfaces to 20% H2O2 for 2 h increases the wettability of the surfaces, but also seems to increase the monoclinic phase, especially on roughened surfaces, which can be considered detrimental to material stability. Moreover, the H2O2-treatment has no influence on osteoblast behavior.
Effect of Defects in Graphene/Cu Composites on the Density of States
Authors: Kim Song Mi , Park Woo Rim , Park Jun Seok , Song Sang Min , Kwon Oh Heon
Published: January 2023

The process of handling and bonding copper (Cu) and graphene inevitably creates defects. To use graphene/Cu composites as electronic devices with new physical properties, it is essential to evaluate the effect of such defects. Since graphene is an ultrathin anisotropic material having a hexagonal structure, an evaluation of graphene/Cu composites containing defects was conducted taking into account the inherent structural characteristics. The purpose of this study is to evaluate defects that may occur in the manufacturing process and to present a usable basic method for the stable design research and development of copper/graphene composites essential for commercialization of copper/graphene composites. In the future, when performing analytical calculations on various copper/graphene composites and defect shapes in addition to the defect conditions presented in this paper, it is considered that it can be used as a useful method considering defects that occur during application to products of desired thickness and size. Herein, density functional theory was used to evaluate the behavior of graphene/Cu composites containing defects. The density of states (DOS) values were also calculated. The analysis was implemented using three kinds of models comprising defect-free graphene and two- and four-layered graphene/Cu composites containing defects. DOS and Fermi energy levels were used to gage the effect of defects on electrical properties.
Asphalt Mixtures Fatigue Life Considering Various Environmental Impacts
Authors: Mączka Eryk , Mackiewicz Piotr
Published: January 2023

The pavement structure during the colder seasons (winter) or in regions located above sea level is commonly affected and deteriorated by many environmental factors. Two prominent factors are water and frost (weather) or road salt (maintenance). According to the article’s literature review, there are only a few studies related to water and frost or road salt impact on mineral asphalt mixes considering fatigue. Most of the tests were performed on mixes containing common road asphalt or only one binder content level was investigated. There are no articles that investigate this problem comprehensively including new asphalt, its content levels, or production technology. Based on the literature review, the main problem regarding degradation impact on mixtures fatigue life was stated. The investigation was performed using two proprietary experimental methods allowing approximates in situ conditions regarding environmental impacts. A dynamic four-point bending fatigue test was applied to evaluate degradation considering fatigue. The investigation was performed using four coarse-graded asphalt mixtures (asphalt concrete AC 22) which differed in binder type (35/50 WMA, 35/50, 25/55-60, and 25/55-80 HIMA), content level (4.24%, 4.03%, 3.82%), and production technology (hot and warm). Regarding the results obtained, the authors proposed a degradation ratio regarding fatigue life variability. Based on the obtained results and ratio used, it was found that both interactions caused a significant fatigue life decrease—in the worst case, over tens of percent. Furthermore, it was found that asphalt mixture resistance to environmental factors depends on binder type, its content level, air void content, and discussed impact. Moreover, asphalt mixtures’ susceptibility to degradation (fatigue) is extreme at lower binder content levels and accelerates due to air void content increase. In the article, it was also stated that the highest resistance was reached by a mixture with highly modified asphalt (25/55-80 HIMA). It was also found that the SBS polymer dosage increase in the asphalt matrix enhances asphalt mixture resistance to environmental impacts. The least resistant to the environmental degradation mixture was WMA (35/50 WMA).
Internal Flow Characteristics of High-Specific-Speed Centrifugal Pumps with Different Number of Impeller Blades under Large Flow Conditions
Authors: Wang Chuan , Chen Xionghuan , Ge Jie , Cao Weidong , Zhang Qiqi , Zhu Yong , Chang Hao
Published: January 2023

As compared with a conventional centrifugal pump, a high-specific-speed centrifugal pump mostly operates under large flow conditions. In this paper, a typical high-specific-speed centrifugal pump is examined, and the effect of the blade number on the internal flow condition is investigated numerically. The numerical predictions have been verified through measurement. It was found that the predictions and the measurements are in good agreement of discrepancy. Serious cavitation could be observed within the pump when the flow rate reached 1300 m3/h. Meanwhile, the effect of the blade number on the cavitation intensity was extremely obvious. The cavitation area at the inlet edge of the blades significantly reduced when the blade number increased from three to six. In addition, the turbulent kinetic energy within the pump was more uniformly distributed. This demonstrates that the blade number can be reasonably chosen to improve the internal flow pattern within the pump, which could provide a theoretical basis for the practical application of high-specific-speed centrifugal pumps
Performance Evaluation of TBM Using an Improved Load Prediction Model
Authors: Zhou Xinghai , Gong Guofang , Zhang Yakun , Wu Weiqiang , Chen Yuxi
Published: January 2023

Excavation load prediction is of great importance for the prior design and latter performance evaluation of tunnel boring machines (TBMs). In this paper, an improved load prediction model is developed based on classical Colorado school of mines model for TBMs equipped with constant cross-sectional disc cutters. The typical structure and principle are introduced to predict the single cutter force, and the total cutter group load is calculated by defining the equivalent diameter and cutter spacing. Subsequently, the improved model of a more brief and acceptable type is established via summation. Some novel performance indexes, including the reformed field penetration index, torque/thrust penetration index, and specific energy are, respectively, derived in formulaic form. By field data verification in the borehole zones of two cases, the proposed model is proven to be more accurate in the total load prediction. The single-factor regression results show that the reformed field penetration index reveals the nonlinear relationship between TBM load and penetration rate, and the torque/thrust penetration index is a new TBM inherent index to evaluate the working conditions. Specific energy, used to evaluate the excavation efficiency, is positive with rock strength and proved negative with penetration rate via a normalization analysis. Finally, suggestions on the cutter group configuration against abominable stratum are discussed.
A Method of Improving the Length Measurement Accuracy of Metal Parts Using Polarization Vision
Authors: Tan Zhiying , Ji Yan , Fan Wenbo , Kong Weifeng , Tao Xu , Xu Xiaobin , Luo Minzhou
Published: January 2023

Measurement technology based on machine vision has been widely used in various industries. The development of vision measurement technology mainly depends on the process of photosensitive components and the algorithm of processing a target image. In the high-precision dimension measurement of machined metal parts, the high-resolution imaging device usually exposes the cutting texture of the metal surface and affects the accuracy of measurement algorithm. At the same time, the edges of machined metal parts are often chamfered, which makes the edges of objects in the picture overexposed in the lighting measurement environment. These factors reduce the accuracy of dimensioning metal parts using visual measurements. The traditional vision measurement method based on color/gray image makes it difficult to analyze the physical quantities in the light field except for the light intensity, which limits the measurement accuracy. Polarization information can more carefully depict the edge contour edge information in the scene and increase the contrast between the foreground and the background. This paper presents a method to improve the measurement accuracy of machined metal parts by using polarization vision. The incident angle of the light source is optimized according to the complex refractive index of the metal material, and the degree of polarization image with enhanced edge contour features of the ROI (region of interest) is obtained. The high-precision measurement of cylindrical brass motor components is realized by using the method of reprojection transformation correction and maximum correlation template matching (NCC) for rough positioning, as well as the method of edge extraction and optimal fitting. The experimental results show that for copper parts with a tolerance range of ±0.1 mm, the average measurement error and maximum measurement error are within 0.01 mm, which are higher than the existing color/gray image measurement methods.
A Survey on the Theory of n-Hypergroups
Authors: Davvaz Bijan , Leoreanu-Fotea Violeta , Vougiouklis Thomas
Published: January 2023

This paper presents a series of important results from the theory of n-hypergroups. Connections with binary relations and with lattices are presented. Special attention is paid to the fundamental relation and to the commutative fundamental relation. In particular, join n-spaces are analyzed.
Marine-Derived Natural Product HDYL-GQQ-495 Targets P62 to Inhibit Autophagy
Authors: Li Quanfu , Fan Jianjun , Chen Yinghan , Liu Yiyang , Liu Hang , Jiang Wei , Li Dehai , Dang Yongjun
Published: January 2023

Autophagy is widely implicated in pathophysiological processes such as tumors and metabolic and neurodegenerative disorders, making it an attractive target for drug discovery. Several chemical screening approaches have been developed to uncover autophagy-modulating compounds. However, the modulation capacity of marine compounds with significant pharmacological activities is largely unknown. We constructed an EGFPKI-LC3B cell line using the CRISPR/Cas9 knock-in strategy in which green fluorescence indicated endogenous autophagy regulation. Using this cell line, we screened a compound library of approximately 500 marine natural products and analogues to investigate molecules that altered the EGFP fluorescence. We identified eight potential candidates that enhanced EGFP fluorescence, and HDYL-GQQ-495 was the leading one. Further validation with immunoblotting demonstrated that cleaved LC3 was increased in dose- and time-dependent manners, and the autophagy adaptor P62 showed oligomerization after HDYL-GQQ-495 treatment. We also demonstrated that HDYL-GQQ-495 treatment caused autophagy substrate aggregation, which indicated that HDYL-GQQ-495 serves as an autophagy inhibitor. Furthermore, HDYL-GQQ-495 induced Gasdermin E (GSDME) cleavage and promoted pyroptosis. Moreover, HDYL-GQQ-495 directly combined with P62 to induce P62 polymerization. In P62 knockout cells, the cleavage of LC3 or GSDME was blocked after HDYL-GQQ-495 treatment. The EGFPKI-LC3B cell line was an effective tool for autophagy modulator screening. Using this tool, we found a novel marine-derived compound, HDYL-GQQ-495, targeting P62 to inhibit autophagy and promote pyroptosis.