bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and Validation of a Measure of Quality in Playgroups: Playgroups Environment Rating Scale
Authors: Russo Vanessa , Barata M. Clara , Alexandre Joana , Leitão Catarina , de Sousa Bruno
Published: May 2022

Playgroups are community-based services that bring together young children and their caregivers for the purpose of play and social activities. Preliminary evidence shows that playgroup impacts may be dependent on the quality of the playgroup. However, to date, there is no reliable and valid measure of playgroup quality. In this paper we describe the development and validation of the Playgroup Environment Rating Scale (PERS), a standardized observation measure designed to assess the quality of playgroups. PERS builds on traditional measures used to evaluate the quality of formal settings of early childhood education and care, while proposing to assess dimensions of quality specific to the nature of playgroups, namely complex interactions between several types of participants. After developing and testing the observation measure on 24 playgroup videos, we analyzed the psychometric properties. Results showed that the PERS had good interrater reliability, was internally consistent and shows a good preliminary factor structure. Tests for convergent and criterion-related validity also presented promising results. The process of design guaranteed that the PERS can be applied to different contexts of playgroups and may also be useful for informing service planning and practice. Further national and international validation will help replicate the validity of the scale.
Survival Foreign Language Acquisition Strategies During the Emergency Remote Learning: An Exploratory Study in Molding Indonesian Students’ Creativity
Authors: Mufidah Nida , Yansyah  , Jumadi
Published: May 2022

Being a foreign language, mastery of English acquisition is hard for Indonesian learners. The transformation of teaching and learning in the condition of the COVID-19 outbreak even makes the teaching-and-learning is more difficult since they previously rely on face-to-face interaction and listening to teachers as the primary source of learning with unconvincing result. This article explores how undergraduate students cope up with emergency remote learning. Using exploratory research design, the students learning strategies are identified. Sixty-four university students who experienced blended learning using Google Classroom for one semester were recruited to participate in the research. A questionnaire and interview were used to collect the data. The questionnaire was developed to examine the kinds of effective strategies employed by the students. The interview aimed to detail their responses so their strategies can be mapped clearly. The research findings showed that some learning strategies, such as social and cognitive strategies, are more favorable than others during the pandemic. The condition requires them to make some changes; even some students found some new techniques for learning. At the end of the article, some implications for implementing future blended or online learning are highlighted.
Immune Related Adverse Events of the Thyroid – A Narrative Review
Authors: Muir Christopher A. , Tsang Venessa H. M. , Menzies Alexander M. , Clifton-Bligh Roderick J.
Published: May 2022

Immune checkpoints are small molecules present on the cell surface of T-lymphocytes. They maintain self-tolerance and regulate the amplitude and duration of T-cell responses. Antagonism of immune checkpoints with monoclonal antibodies (immune checkpoint inhibitors) is a rapidly evolving field of anti-cancer immunotherapy and has become standard of care in management of many cancer subtypes. Immune checkpoint inhibition is an effective cancer treatment but can precipitate immune related adverse events (irAEs). Thyroid dysfunction is the most common endocrine irAE and can occur in up to 40% of treated patients. Both thyrotoxicosis and hypothyroidism occur. The clinical presentation and demographic associations of thyrotoxicosis compared to hypothyroidism suggest unique entities with different etiologies. Thyroid irAEs, particularly overt thyrotoxicosis, are associated with increased immune toxicity in other organ systems, but also with longer progression-free and overall survival. Polygenic risk scores using susceptibility loci associated with autoimmune thyroiditis predict development of checkpoint inhibitor associated irAEs, suggesting potentially shared mechanisms underpinning their development. Our review will provide an up-to-date summary of knowledge in the field of thyroid irAEs. Major focus will be directed toward pathogenesis (including genetic factors shared with autoimmune thyroid disease), demographic associations, clinical presentation and course, treatment, and the relationship with cancer outcomes.
A New Way Out of the Predicament of Anaplastic Thyroid Carcinoma From Existing Data Analysis
Authors: Zhou Yikun , Zhao Yang , Ding Xi , Liang Jing , Xu Huayang , Lin Yuxuan , Khan Hamad Haider , Shi Bingyin
Published: May 2022

Background

Anaplastic thyroid carcinoma (ATC) is an endocrine tumor with a low incidence but a very poor prognosis. The vast majority of patients have a survival time of only three to six months, but a few survive for two years or more. In recent years, there have been major breakthroughs in targeted and immunotherapy in the field of oncology therapy. Although the preliminary study for ATC showed a promising prospect, more clinical trials are needed. It is the best approach to explore the measures that can improve survival time of ATC from the available clinical data, especially those with long survival.

Methods

We report on an 82-year-old ATC patient who survived for 3 years and systematically review the clinical characteristics of 45 ATC patients with complete data from the two largest centers in northwest China. In particular, factors related to long-term survival were analyzed and summarized.

Results

Three years prior, an 82-year-old woman was diagnosed with ATC by core needle biopsy following a physical examination. The thyroid tumor was resected within one month, and then the patient was treated with radiotherapy. The patient was still healthy after three years of follow-up. Analysis of prognostic factors for the 45 reviewed patients showed that those undergoing radical surgery (median overall survival (OS) = 472 days, p = 0.0261) and radiotherapy (median OS = 220 days, p = 0.0136) had better outcomes. In addition, patients younger than 65 years (median OS = 164.5 days, p = 0.0176) and with a lower tumor stage (IV A, median OS = 633.5 days, p = 0.0191) also had a better outcome.

Conclusion

ATC is a highly malignant tumor, but timely early diagnosis and standardized treatment with radical surgery and radiotherapy as the core can achieve good results. Some patients can achieve long-term survival.
Articular Cartilage Assessment Using Ultrashort Echo Time MRI: A Review
Authors: Afsahi Amir Masoud , Sedaghat Sam , Moazamian Dina , Afsahi Ghazaleh , Athertya Jiyo S. , Jang Hyungseok , Ma Ya-Jun
Published: May 2022

Articular cartilage is a major component of the human knee joint which may be affected by a variety of degenerative mechanisms associated with joint pathologies and/or the aging process. Ultrashort echo time (UTE) sequences with a TE less than 100 µs are capable of detecting signals from both fast- and slow-relaxing water protons in cartilage. This allows comprehensive evaluation of all the cartilage layers, especially for the short T2 layers which include the deep and calcified zones. Several ultrashort echo time (UTE) techniques have recently been developed for both morphological imaging and quantitative cartilage assessment. This review article summarizes the current catalog techniques based on UTE Magnetic Resonance Imaging (MRI) that have been utilized for such purposes in the human knee joint, such as T1, T2∗, T1ρ, magnetization transfer (MT), double echo steady state (DESS), quantitative susceptibility mapping (QSM) and inversion recovery (IR). The contrast mechanisms as well as the advantages and disadvantages of these techniques are discussed.
Multi-Sine EIS for Early Detection of PEMFC Failure Modes
Authors: Fortin Patrick , Gerhardt Michael R. , Ulleberg Øystein , Zenith Federico , Holm Thomas
Published: May 2022

Electrochemical impedance spectroscopy (EIS) is a powerful technique that can be used to detect small changes in electrochemical systems and subsequently identify the source of the change. While promising, analysis is often non-intuitive and time-consuming, where collection times of a single EIS spectrum can reach several minutes. To circumvent the long collection times associated with traditional EIS measurements, a multi-sine EIS technique was proposed in which the simultaneous application of many frequencies can reduce the acquisition time to less than a minute. This shortened acquisition time opens the possibility to use multi-sine EIS as a real-time diagnostic tool for monitoring the state-of-health of commercial fuel cell systems. In this work, a single-cell proton exchange membrane fuel cell (PEMFC) was characterised using multi-sine EIS, by establishing steady-state impedance response under baseline conditions before systematically changing operating conditions and monitoring the dynamic changes of the impedance response. Our initial results demonstrate that full multi-sine EIS spectra, encompassing a frequency range from 50 kHz to 0.5 Hz, can be collected and analysed using simple equivalent circuit models in 50 s. It is shown that this timeframe is sufficiently short to capture the dynamic response of the fuel cell in response to changing operating conditions, thereby validating the use of multi-sine EIS as a diagnostic technique for in-situ monitoring and fault detection during fuel cell operation.
Impact of Regional Development Strategy on the Productivity of Polluting Firms:Evidence From China
Authors: Dou Jianmin , Tao Zhipeng , Ji Yongbao
Published: May 2022

This study aims to analyse the heterogeneous impact of the China’s Western Development Strategy (WDS) on the productivity of different types of firms. Based on the heterogeneous firm model and simulation, this study utilizes the WDS as a quasi-natural experiment and uses the difference-in-difference-in-differences (DDD) method to study the heterogeneous impact on the productivity of polluting and non-polluting firms. The WDS has increased the productivity of polluting firms in the western region by 8.0–12.6%, compared to the central region and non-polluting industries. This phenomenon is the result of the environmental cost effect and the migration effect, but not the result of the Porter effect. In addition, this study shows that the heterogeneity of productivity improvement in state-owned firms, large-scale firms, and firms not located in the acid rain and sulfur dioxide control zones is more significant. The study suggests that it is necessary to implement industry-differentiated regional policies, implement different regional preferential policies for polluting firms, and to strengthen environmental regulations, so as to achieve a win-win situation between firm efficiency improvement and environmental protection.
Biological Toxicity of Heavy Metal(loid)s in Natural Environments: From Microbes to Humans
Authors: Ding Chunlian , Chen Jianxin , Zhu Feng , Chai Liyuan , Lin Zhang , Zhang Kejing , Shi Yan
Published: May 2022

Excessive levels of heavy metal(loid)s (HMs) in natural environments pose a serious threat to living beings worldwide. HM exposure causes irreversible damage to structural components and metabolic processes in living organisms, as has been observed in multiple studies on various organisms. In the natural environment, biological individuals interact with others through the food web rather than exist independently, which facilitates the transfer of HMs in the food web. However, the difference in HM toxicity among different biological species has not been elucidated. This review provides information on the speciation and migration of HMs in different environments to clarify the HM exposure routes of different biological species. The differences in the biotoxicity of HMs to different species, from microbes to humans, are emphasized. The relationship between HM toxicity and biological species is confirmed by the fact that HMs can be transferred and bioaccumulated along the food chain. Effective strategies for decreasing HMs emissions and removing HMs from the environment are briefly discussed. Finally, the limitations of the present study and future perspectives are discussed.
Spillover Effects of Built-Up Land Expansion Under Ecological Security Constraint at Multiple Spatial Scales
Authors: Yang Dan , Lou Yuanyuan , Zhang Pengyan , Jiang Ling
Published: May 2022

Land-use change is a global issue, and the built-up land expansion has affected the ecological landscape patterns of the major river basins in the world. However, measurement of the ecological risks of potential landscape and identification of the dynamic relationships by natural and human-driven built-up land expansion at different zoning scales are still less understood. Based on multi-period Landsat satellite image data, we combined remote sensing (RS) and geography information systems (GIS) technologies with Spatial Durbin Panel Model to quantitatively analyze the landscape ecological effects under the built-up land expansion in the Yellow River Basin. The results showed that there is spatial heterogeneity in the built-up land expansion and ecological security patterns, with the expansion gravity center gradually spreading from the downstream to the middle and upstream areas, and the most dramatic change in landscape patches of ecological safety patterns occurring around the year 2000. At different zoning scales, there is a spatial spillover effect on the interaction between built-up land expansion and ecological security, with the significance of the regression estimates decreasing from large sample sizes to small sample sizes. Our findings highlighted the importance of spatial heterogeneity at different zoning scales in identifying the dynamic relationship between built-up land expansion and ecological security, scientific planning of land resources, and mitigation of ecological and environmental crises.
The Chemokine Receptor CCR8 Is a Target of Chimeric Antigen T Cells for Treating T Cell Malignancies
Authors: Zheng Diwei , Wang Xindong , Cheng Lin , Qin Le , Jiang Zhiwu , Zhao Ruocong , Li Yao , Shi Jingxuan , Wu Qiting , Long Youguo , Wang Suna , Tang Zhaoyang , Wei Wei , Yang Jie , Li Yangqiu , Zhou Hongsheng , Liu Qifa , Liu Pentao , Chen Xinwen , Yao Yao , Yang LiHua , Li Peng
Published: May 2022

Chimeric antigen receptor (CAR) T cells have been successfully used in the therapy of B cell leukemia and lymphoma, but still have many challenges in their use for treating T cell malignancies, such as the lack of unique tumor antigens, their limitation of T cell expansion, and the need for third party donors or genome editing. Therefore, we need to find novel targets for CAR T cell therapy to overcome these challenges. Here, we found that both adult T-cell leukemia/lymphoma (ATLL) patients and ATLL cells had increased CCR8 expression but did not express CD7. Moreover, targeting CCR8 in T cells did not impair T cell expansion in vitro. Importantly, anti-CCR8 CAR T cells exhibited antitumor effects on ATLL- and other CCR8-expressing T-ALL cells in vitro and in vivo, and prolonged the survival of ATLL and Jurkat tumor-bearing mouse models. In conclusion, these collective results show that anti-CCR8 CAR T cells possess strong antitumor activity and represent a promising therapeutic approach for ATLL and CCR8+ tumors.
Phenotypic Displays of Cholinergic Enzymes Associate With Markers of Inflammation, Neurofibrillary Tangles, and Neurodegeneration in Pre- and Early Symptomatic Dementia Subjects
Authors: Teitsdottir Unnur D. , Darreh-Shori Taher , Lund Sigrun H. , Jonsdottir Maria K. , Snaedal Jon , Petersen Petur H.
Published: May 2022

Background

Cholinergic drugs are the most commonly used drugs for the treatment of Alzheimer’s disease (AD). Therefore, a better understanding of the cholinergic system and its relation to both AD-related biomarkers and cognitive functions is of high importance.

Objectives

To evaluate the relationships of cerebrospinal fluid (CSF) cholinergic enzymes with markers of amyloidosis, neurodegeneration, neurofibrillary tangles, inflammation and performance on verbal episodic memory in a memory clinic cohort.

Methods

In this cross-sectional study, 46 cholinergic drug-free subjects (median age = 71, 54% female, median MMSE = 28) were recruited from an Icelandic memory clinic cohort targeting early stages of cognitive impairment. Enzyme activity of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) was measured in CSF as well as levels of amyloid-β1–42 (Aβ42), phosphorylated tau (P-tau), total-tau (T-tau), neurofilament light (NFL), YKL-40, S100 calcium-binding protein B (S100B), and glial fibrillary acidic protein (GFAP). Verbal episodic memory was assessed with the Rey Auditory Verbal Learning (RAVLT) and Story tests.

Results

No significant relationships were found between CSF Aβ42 levels and AChE or BuChE activity (p > 0.05). In contrast, T-tau (r = 0.46, p = 0.001) and P-tau (r = 0.45, p = 0.002) levels correlated significantly with AChE activity. Although neurodegeneration markers T-tau and NFL did correlate with each other (r = 0.59, p < 0.001), NFL did not correlate with AChE (r = 0.25, p = 0.09) or BuChE (r = 0.27, p = 0.06). Inflammation markers S100B and YKL-40 both correlated significantly with AChE (S100B: r = 0.43, p = 0.003; YKL-40: r = 0.32, p = 0.03) and BuChE (S100B: r = 0.47, p < 0.001; YKL-40: r = 0.38, p = 0.009) activity. A weak correlation was detected between AChE activity and the composite score reflecting verbal episodic memory (r = −0.34, p = 0.02). LASSO regression analyses with a stability approach were performed for the selection of a set of measures best predicting cholinergic activity and verbal episodic memory score. S100B was the predictor with the highest model selection frequency for both AChE (68%) and BuChE (73%) activity. Age (91%) was the most reliable predictor for verbal episodic memory, with selection frequency of both cholinergic enzymes below 10%.

Conclusions

Results indicate a relationship between higher activity of the ACh-degrading cholinergic enzymes with increased neurodegeneration, neurofibrillary tangles and inflammation in the stages of pre- and early symptomatic dementia, independent of CSF Aβ42 levels.
Reorganized Brain Functional Network Topology in Presbycusis
Authors: Guan Bing , Xu Yixi , Chen Yu-Chen , Xing Chunhua , Xu Li , Shang Song'an , Xu Jin-Jing , Wu Yuanqing , Yan Qi
Published: May 2022

Purpose

Presbycusis is characterized by bilateral sensorineural hearing loss at high frequencies and is often accompanied by cognitive decline. This study aimed to identify the topological reorganization of brain functional network in presbycusis with/without cognitive decline by using graph theory analysis approaches based on resting-state functional magnetic resonance imaging (rs-fMRI).

Methods

Resting-state fMRI scans were obtained from 30 presbycusis patients with cognitive decline, 30 presbycusis patients without cognitive decline, and 50 age-, sex-, and education-matched healthy controls. Graph theory was applied to analyze the topological properties of brain functional networks including global and nodal metrics, modularity, and rich-club organization.

Results

At the global level, the brain functional networks of all participants were found to possess small-world properties. Also, significant group differences in global network metrics were observed among the three groups such as clustering coefficient, characteristic path length, normalized characteristic path length, and small-worldness. At the nodal level, several nodes with abnormal betweenness centrality, degree centrality, nodal efficiency, and nodal local efficiency were detected in presbycusis patients with/without cognitive decline. Changes in intra-modular connections in frontal lobe module and inter-modular connections in prefrontal subcortical lobe module were found in presbycusis patients exposed to modularity analysis. Rich-club nodes were reorganized in presbycusis patients, while the connections among them had no significant group differences.

Conclusion

Presbycusis patients exhibited topological reorganization of the whole-brain functional network, and presbycusis patients with cognitive decline showed more obvious changes in these topological properties than those without cognitive decline. Abnormal changes of these properties in presbycusis patients may compensate for cognitive impairment by mobilizing additional neural resources.
Altered Structural and Functional Patterns Within Executive Control Network Distinguish Frontal Glioma-Related Epilepsy
Authors: Di Guangfu , Tan Mingze , Xu Rui , Zhou Wei , Duan Kaiqiang , Hu Zongwen , Cao Xiaoxiang , Zhang Hongchuang , Jiang Xiaochun
Published: May 2022

Background

The tumor invasion of the frontal lobe induces changes in the executive control network (ECN). It remains unclear whether epileptic seizures in frontal glioma patients exacerbate the structural and functional alterations within the ECN, and whether these changes can be used to identify glioma-related seizures at an early stage. This study aimed to investigate the altered structural and functional patterns of ECN in frontal gliomas without epilepsy (non-FGep) and frontal gliomas with epilepsy (FGep) and to evaluate whether the patterns can accurately distinguish glioma-related epilepsy.

Methods

We measured gray matter (GM) volume, regional homogeneity (ReHo), and functional connectivity (FC) within the ECN to identify the structural and functional changes in 50 patients with frontal gliomas (29 non-FGep and 21 FGep) and 39 healthy controls (CN). We assessed the relationships between the structural and functional changes and cognitive function using partial correlation analysis. Finally, we applied a pattern classification approach to test whether structural and functional abnormalities within the ECN can distinguish non-FGep and FGep from CN subjects.

Results

Within the ECN, non-FGep and FGep showed increased local structure (GM) and function (ReHo), and decreased FC between brain regions compared to CN. Also, non-FGep and FGep showed differential patterns of structural and functional abnormalities within the ECN, and these abnormalities are more severe in FGep than in non-FGep. Lastly, FC between the right superior frontal gyrus and right dorsolateral prefrontal cortex was positively correlated with episodic memory scores in non-FGep and FGep. In particular, the support vector machine (SVM) classifier based on structural and functional abnormalities within ECN could accurately distinguish non-FGep and FGep from CN, and FGep from non-FGep on an individual basis with very high accuracy, area under the curve (AUC), sensitivity, and specificity.

Conclusion

Tumor invasion of the frontal lobe induces local structural and functional reorganization within the ECN, exacerbated by the accompanying epileptic seizures. The ECN abnormalities can accurately distinguish the presence or absence of epileptic seizures in frontal glioma patients. These findings suggest that differential ECN patterns can assist in the early identification and intervention of epileptic seizures in frontal glioma patients.
Contribution of the Tumor Microenvironment to Metabolic Changes Triggering Resistance of Multiple Myeloma to Proteasome Inhibitors
Authors: Schwestermann Jonas , Besse Andrej , Driessen Christoph , Besse Lenka
Published: May 2022

Virtually all patients with multiple myeloma become unresponsive to treatment with proteasome inhibitors over time. Relapsed/refractory multiple myeloma is accompanied by the clonal evolution of myeloma cells with heterogeneous genomic aberrations, diverse proteomic and metabolic alterations, and profound changes of the bone marrow microenvironment. However, the molecular mechanisms that drive resistance to proteasome inhibitors within the context of the bone marrow microenvironment remain elusive. In this review article, we summarize the latest knowledge about the complex interaction of malignant plasma cells with its surrounding microenvironment. We discuss the pivotal role of metabolic reprograming of malignant plasma cells within the tumor microenvironment with a subsequent focus on metabolic rewiring in plasma cells upon treatment with proteasome inhibitors, driving multiple ways of adaptation to the treatment. At the same time, mutual interaction of plasma cells with the surrounding tumor microenvironment drives multiple metabolic alterations in the bone marrow. This provides a tumor-promoting environment, but at the same time may offer novel therapeutic options for the treatment of relapsed/refractory myeloma patients.
Comparison of Dexmedetomidine Versus Propofol in Mechanically Ventilated Patients With Sepsis: A Meta-Analysis of Randomized Controlled Trials
Authors: Ding Hua-Ze , Dong Yi-Ling , Zhang Kai-Yue , Bai Jia-Yu
Published: May 2022

Purpose: The aim of the present study was to evaluate the effects of dexmedetomidine compared with propofol in mechanically ventilated patients with sepsis.

Methods: We searched PubMed, EMBASE, and Cochrane Library for randomized controlled trials comparing the effects of dexmedetomidine versus propofol in septic patients requiring mechanical ventilation from inception to December 2021. The primary outcome was 28/30-day mortality and secondary outcomes were ventilator-free days and the length of ICU stay. Pooled relative risk (RR), mean deviation (MD), along with 95% confidence intervals (CI) were used to express outcomes by the software of Review Manager 5.3.

Results: Seven studies with a total of 1,212 patients were eligible for meta-analysis. The results primarily showed that dexmedetomidine had no significant effects on the 28/30-day mortality (RR = 1.04 [0.85–1.26], p = 0.70, I2 = 3%). As for secondary outcomes, the administration of dexmedetomidine was not associated with longer-ventilator-free days (MD = 0.50 [−2.15, 3.15], p = 0.71, I2 = 24%) compared with propofol. However, our results revealed dexmedetomidine could shorten the length of ICU stay (MD = −0.76 [−1.34, −0.18], p = 0.01, I2 = 33%).

Conclusion: Administration of dexmedetomidine for sedation in septic patients who required mechanical ventilation had no effect on 28/30-day mortality and ventilator-free days, but it could shorten the length of ICU stay.
Hypertrophic Cardiomyopathy Mutations of Troponin Reveal Details of Striated Muscle Regulation
Authors: Chalovich J. M. , Zhu L. , Johnson D.
Published: May 2022

Striated muscle contraction is inhibited by the actin associated proteins tropomyosin, troponin T, troponin I and troponin C. Binding of Ca2+ to troponin C relieves this inhibition by changing contacts among the regulatory components and ultimately repositioning tropomyosin on the actin filament creating a state that is permissive for contraction. Several lines of evidence suggest that there are three possible positions of tropomyosin on actin commonly called Blocked, Closed/Calcium and Open or Myosin states. These states are thought to correlate with different functional states of the contractile system: inactive-Ca2+-free, inactive-Ca2+-bound and active. The inactive-Ca2+-free state is highly occupied at low free Ca2+ levels. However, saturating Ca2+ produces a mixture of inactive and active states making study of the individual states difficult. Disease causing mutations of troponin, as well as phosphomimetic mutations change the stabilities of the states of the regulatory complex thus providing tools for studying individual states. Mutants of troponin are available to stabilize each of three structural states. Particular attention is given to the hypertrophic cardiomyopathy causing mutation, Δ14 of TnT, that is missing the last 14 C-terminal residues of cardiac troponin T. Removal of the basic residues in this region eliminates the inactive-Ca2+-free state. The major state occupied with Δ14 TnT at inactivating Ca2+ levels resembles the inactive-Ca2+-bound state in function and in displacement of TnI from actin-tropomyosin. Addition of Ca2+, with Δ14TnT, shifts the equilibrium between the inactive-Ca2+-bound and the active state to favor that latter state. These mutants suggest a unique role for the C-terminal region of Troponin T as a brake to limit Ca2+ activation.
Clustered Regularly Interspaced Short Palindromic Repeats-Associated Protein System for Resistance Against Plant Viruses: Applications and Perspectives
Authors: Silva Fredy D. A. , Fontes Elizabeth P. B.
Published: May 2022

Different genome editing approaches have been used to engineer resistance against plant viruses. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas; CRISPR/Cas) systems to create pinpoint genetic mutations have emerged as a powerful tool for molecular engineering of plant immunity and increasing resistance against plant viruses. This review presents (i) recent advances in engineering resistance against plant viruses by CRISPR/Cas and (ii) an overview of the potential host factors as targets for the CRISPR/Cas system-mediated broad-range resistance and immunity. Applications, challenges, and perspectives in enabling the CRISPR/Cas system for crop protection are also outlined.
WATER-SOAKED SPOT1 Controls Chloroplast Development and Leaf Senescence via Regulating Reactive Oxygen Species Homeostasis in Rice
Authors: Xu Jiangmin , Ji Zhiyuan , Wang Chunlian , Xu Feifei , Wang Fujun , Zheng Yuhan , Tang Yongchao , Wei Zheng , Zhao Tianyong , Zhao Kaijun
Published: May 2022

Transmembrane kinases (TMKs) play important roles in plant growth and signaling cascades of phytohormones. However, its function in the regulation of early leaf senescence (ELS) of plants remains unknown. Here, we report the molecular cloning and functional characterization of the WATER-SOAKED SPOT1 gene which encodes a protein belongs to the TMK family and controls chloroplast development and leaf senescence in rice (Oryza sativa L.). The water-soaked spot1 (oswss1) mutant displays water-soaked spots which subsequently developed into necrotic symptoms at the tillering stage. Moreover, oswss1 exhibits slightly rolled leaves with irregular epidermal cells, decreased chlorophyll contents, and defective stomata and chloroplasts as compared with the wild type. Map-based cloning revealed that OsWSS1 encodes transmembrane kinase TMK1. Genetic complementary experiments verified that a Leu396Pro amino acid substitution, residing in the highly conserved region of leucine-rich repeat (LRR) domain, was responsible for the phenotypes of oswss1. OsWSS1 was constitutively expressed in all tissues and its encoded protein is localized to the plasma membrane. Mutation of OsWSS1 led to hyper-accumulation of reactive oxygen species (ROS), more severe DNA fragmentation, and cell death than that of the wild-type control. In addition, we found that the expression of senescence-associated genes (SAGs) was significantly higher, while the expression of genes associated with chloroplast development and photosynthesis was significantly downregulated in oswss1 as compared with the wild type. Taken together, our results demonstrated that OsWSS1, a member of TMKs, plays a vital role in the regulation of ROS homeostasis, chloroplast development, and leaf senescence in rice.
Teachers' Value Consonance and Employee-Based Brand Equity: The Mediating Role of Belongingness and Self-Efficacy
Authors: Yang Xianbi , Hussain Qureshi Abrar , Kuo Yenku , Quynh Nguyen Ngoc , Kumar Tribhuwan , Wisetsri Worakamol
Published: May 2022

This study investigated the impact of value consonance on employee-based brand equity through the mediating role of teachers' self-efficacy and belongingness. For this purpose, a deductive approach was followed, and data were collected under a cross-sectional research design from academia through a questionnaire. Prior approval from the administration was sought before administrating the questionnaire on a large scale and a sample of 520 teachers was approached in the first phase. At this stage, 418 answered questionnaires were received, while in the second wave, questions related to the teacher's self-efficacy and employee-based brand equity were asked from the respondents. Out of these 418 re-distributed questionnaires, 387 were received back and after discarding the partially filled and incomplete questionnaires, the useable sample size was left as 372. Data have been analyzed by using the structural equation modeling technique, which was assessed through measurement and structural model. Results indicate that value consonance can promote positive behaviors in the workplace. Moreover, teachers with high self-efficacy can develop based on brand equity. Similarly, employees with high-value consonance develop a sense of belongingness with their academic institutes. Limitations and future directions are also discussed.
Chronotypes, Sleep and Mental Distress Among Chinese College Students: A Cross-Sectional Study
Authors: Wang Jiajia , Liu Shuai , Guo Junlong , Xiao Rong , Yu Jia , Luo Xian , Xu Yan , Zhao Yuhan , Cui Yingru , Gu Yue , Cai Lidan , Zhang Bin
Published: May 2022

Objective

This study aimed to investigate the chronotypes and their relationship with sleep disturbances and mental distress among college students.

Methods

Students from a university in Guangzhou, China, were recruited through a cross-sectional online survey. Data were collected by self-reported questionnaires including socio-demographics, lifestyles and health conditions, sleep patterns on weekdays and weekends, as well as the reduced Morningness-Eveningness Questionnaire (rMEQ), the Insomnia Severity Index, the Epworth Sleepiness Scale, the Beck Depression Inventory-13, and the Zung Self-Rating Anxiety Scale. Multivariate analyses were performed to examine the associations of chronotypes with sleep compensation, sleep disturbances, and mental distress.

Results

A total of 1,607 questionnaires were received, among which 1,569 (97.6%) were valid for further analysis. Among these participants [mean age 19.86 ± 1.16 (15–27) years], morning types (M-types), intermediate types (I-types), and evening types (E-types) accounted for 14.9, 71.5, and 13.6%, respectively. The regression analysis revealed that E-types were positively associated with long sleep compensation on weekends (adjusted OR 2.443, 95%CI 1.740-3.429) compared with I-types, while M-types were the opposite (adjusted OR 0.623, 95%CI 0.392–0.990). E-types were also positively correlated with insomnia symptoms (adjusted OR 2.000, 95%CI 1.428–2.801), depressive symptoms (adjusted OR 2.068, 95%CI 1.496–2.858), and anxiety symptoms (adjusted OR 2.188, 95%CI 1.387–3.451). However, no significant association was found between chronotypes and excessive daytime sleepiness.

Conclusion

Our study found that E-types were associated with long sleep compensation on weekends and insomnia, depression, and anxiety symptoms. Our findings emphasized the importance of early recognition and intervention of E-types and their accompanied sleep problems and mental distress.
School Meal Programs in Africa: Regional Results From the 2019 Global Survey of School Meal Programs
Authors: Wineman Ayala , Ekwueme Moses C. , Bigayimpunzi Liliane , Martin-Daihirou Alice , de Gois V. N. Rodrigues Eth Ludmilla , Etuge Priscilia , Warner Yale , Kessler Heidi , Mitchell Arlene
Published: May 2022

Introduction

School meal programs operate throughout Africa, serving as a social safety net and aiming to improve children's nutrition, influence their dietary choices, and strengthen the agrifood economy through local procurement. Despite their rapid expansion in the past decade, there has been no systematic effort to comprehensively document school feeding activities across the continent.

Methods

Detailed information on school feeding activities in each country was captured in the Global Survey of School Meal Programs©, which launched in 2019. An invitation to participate was extended to each government, which appointed a national-level respondent to gather information on every large-scale school meal program in the country.

Results

Forty-one countries in Africa (38 in sub-Saharan Africa) responded to the survey in 2019 with information on 68 large-scale programs that together reached 60.1 million children. Across these countries, the aggregate school feeding budget was USD 1.3 billion. Diversity in school meal programs is evident across regions, country income levels, and levels of national commitment. Coverage rates tended to be highest in southern Africa, in countries with school feeding as a line item in the national budget, and in countries with the greatest domestic share of the school feeding budget. Diversity in the school menu tended to be greatest in programs that sourced food through domestic purchase rather than relying on foreign in-kind donations. To address micronutrient malnutrition, about two-thirds of the programs served fortified foods, and one-quarter included micronutrient supplements. Even as rates of overweight/obesity are rising among African school children, just 10% of school meal programs identified its prevention as an objective.

Conclusion

The extent to which school meal programs in Africa are supported with domestic funding reflects a dramatic shift in favor of national ownership and domestic food procurement. At the same time, programs have grappled with inadequate and unpredictable budgets and challenges related to supply chains and logistics—impediments that need to be addressed if these programs are to achieve their objectives. Overall, the survey results underscore the important position of school meal programs within African food systems and their potential (if well-designed) to sustainably improve food security, child health, and nutrition.
Occupational Hazards of Surgical Smoke and Achieving a Smoke Free Operating Room Environment: Asia-Pacific Consensus Statement on Practice Recommendations
Authors: Heroor Anil Ashok , Asaf Belal Bin , Deo Suryanarayana S. V. , Lau Eric Hui-Lun , Mok Chi Wei , DiPasco Peter Joseph , Jain Pradeep , Anand Utpal
Published: May 2022

Background:

Surgical smoke generated through energy devices may present detrimental effects on individuals present in the operating room (OR). Despite the concerns possibly associated with surgical smoke, there may be no mandatory policies that suggest protective measures and limited firm standards are committed yet to address the same.

Aim

The aim of this paper is to present recommendations for surgeons and OR personnel by taking a consensus approach based on available literature and its interpretation by a multi-national panel of experts.

Methods

The Asia-Pacific (APAC) group was established with the aims of reviewing literature evidence, discussing key issues regarding surgical smoke and its hazards, and offering a summary of statements in achieving a smoke-free OR environment. Eleven expert surgeons from the international APAC region were gathered with the purpose of coming to a consensus on engineering, best work-practices, and administrative controls in minimizing surgical smoke exposure. A two-phase consensus method was used to obtain opinions from the expert panel of specialists. Statements with an agreement of more than 80% were accepted.

Findings

For twenty-one statements, the panel achieved consensus on 17 statements; another 5 were dropped due to lack of consensus. The consensus was obtained on statements that address the need for the implementation of administrative policies, training and awareness, standard procedure for the continued use of engineering controls, stringent work practice controls and preventive controls.

Conclusion

The statements presented may guide surgeons and OR personnel in the practical management of surgical smoke safety, mitigating the risks associated with it. The consensus statement also provides a series of recommendations that can be used with other stakeholders, such as policymakers, hospital administrators and professional societies, to highlight and motivate the implementation of meaningful policies.
Treatment of Atlantoaxial Dislocation in Children with Down Syndrome Using Posterior Atlantoaxial Screw Fixation
Authors: Li Chengxin , Tian Yiren , Ren Qiang , Ji Xiangqian , Mao Ziwei , Wu Ming
Published: May 2022

Background

To investigate the effect of posterior atlantoaxial screw fixation for the treatment of atlantoaxial dislocation in children with Down syndrome (DS).

Methods

Children diagnosed with DS who underwent posterior atlantoaxial screw fixation or occipitocervical fusion from January 2017 to January 2020 in Hebei Children’s Hospital were retrospectively included. Preoperative CT and MRI were performed to check the os odontoideum (OsO) and spinal cord compression, signal changes and spinal cord injury grade (ASIA grade).

Results

All 5 children have atlantoaxial dislocation and OsO. Among which 60% (3/5) of children had changes in spinal cord signals and 40% (2/5) had dural sac compression. Every child underwent posterior atlantoaxial screw fixation (3.5-mm diameter), and the average fusion level was 1.8 (1–2). All 5 cases wore the head-neck-chest brace for 3–6 months after the operation. 1 case had dural tear and recovered well after timely suturing. 1 case had internal fixation breakage of the prosthetic joint and underwent revision surgery. At the last follow-up, all cases were fused and the neurological function were all ASIA grade E.

Conclusion

After posterior atlantoaxial screw fixation, fusion and nerve recovery were achieved in all children with atlantoaxial dislocation and OsO. Postoperative head-neck-chest braces are necessary for children, especially those with occipitocervical fusion.
Indocyanine Green Fluorescence Angiography During Laparoscopic Bariatric Surgery: A Pilot Study
Authors: Balla Andrea , Corallino Diletta , Quaresima Silvia , Palmieri Livia , Meoli Francesca , Cordova Herencia Ingrid , Paganini Alessandro M.
Published: May 2022

Aims

Indocyanine green (ICG) fluorescence angiography (FA) is used for several purposes in general surgery, but its use in bariatric surgery is still debated. The objective of the present pilot study is to evaluate the intraoperative utility of ICG-FA during bariatric surgery in order to focus future research on a reliable tool to reduce the postoperative leak rate.

Methods

Thirteen patients (4 men, 30.8%, 9 women, 69.2%) with median age of 52 years (confidence interval, CI, 95% 46.2–58.7 years) and preoperative median body mass index of 42.6 kg/m2 (CI, 95% 36 to 49.3 kg/m2) underwent bariatric surgery with ICG-FA in our center. Three mL of ICG diluted with 10 cc sterile water were intravenously injected after gastric tube creation during laparoscopic sleeve gastrectomy (LSG) and after the gastric pouch and gastro-jejunal anastomosis creation during laparoscopic gastric by-pass (LGB). For the ICG-FA, Karl Storz Image 1S D-Light system (Karl Storz Endoscope GmbH & C. K., Tuttlingen, Germany) placed at a fixed distance of 5 cm from the structures of interest and zoomed vision modality were used to identify the vascular supply. The perfusion pattern was assessed by the surgical team according to a score. The score ranged from 1 (poor vascularization) to 5 (excellent vascularization) based on the intensity and timing of fluorescence of the vascularized structures.

Results

Fom January 2021 to February 2022, six patients underwent LSG (46.2%), three patients underwent LGB (23.1%), and four patients underwent re-do LGB after LSG (30.8%). No adverse effects to ICG were observed. In 11 patients (84.6%) ICG-FA score was 5. During two laparoscopic re-do LGB, the vascular supply was not satisfactory (score 2/5) and the surgical strategy was changed based on ICG-FA (15.4%). At a median follow-up of five months postoperatively, leaks did not occur in any case.

Conclusions

ICG-FA during bariatric surgery is a safe, feasible and promising procedure. It could help to reduce the ischemic leak rate, even if standardization of the procedure and objective fluorescence quantification are still missing. Further prospective studies with a larger sample of patients are required to draw definitive conclusions.
Revised Taxonomy of Rhabdoviruses Infecting Fish and Marine Mammals
Authors: Walker Peter J. , Bigarré Laurent , Kurath Gael , Dacheux Laurent , Pallandre Laurane
Published: May 2022

The Rhabdoviridae is a large family of negative-sense (-) RNA viruses that includes important pathogens of ray-finned fish and marine mammals. As for all viruses, the taxonomic assignment of rhabdoviruses occurs through a process implemented by the International Committee on Taxonomy of Viruses (ICTV). A recent revision of taxonomy conducted in conjunction with the ICTV Rhabdoviridae Study Group has resulted in the establishment of three new subfamilies (Alpharhabdovirinae, Betarhabdovirinae, and Gammarhabdovirinae) within the Rhabdoviridae, as well as three new genera (Cetarhavirus, Siniperhavirus, and Scophrhavirus) and seven new species for viruses infecting fish or marine mammals. All rhabdovirus species have also now been named or renamed to comply with the binomial format adopted by the ICTV in 2021, comprising the genus name followed by a species epithet. Phylogenetic analyses of L protein (RNA-dependent RNA polymerase) sequences of (-) RNA viruses indicate that members of the genus Novirhabdovirus (subfamily Gammarhabdovirinae) do not cluster within the Rhabdoviridae, suggesting the need for a review of their current classification.
Melatonin Alleviates Oxidative Stress Induced by H2O2 in Porcine Trophectoderm Cells
Authors: Fu Yawei , Chen Yue , Jin Zhao , Gao Hu , Song Gang , Wang Qian , Xu Kang
Published: May 2022

Placental oxidative stress has been implicated as a main risk factor for placental dysfunction. Alleviation of oxidative stress and enhancement of antioxidant capacity of porcine trophectoderm (PTr2) cells are effective means to maintaining normal placental function. The present study was conducted to evaluate the protective effect of melatonin (MT) on H2O2-induced oxidative damage in PTr2 cells. Our data revealed that pretreatment with MT could significantly improve the decrease in cell viability induced by H2O2, and reduce intracellular reactive oxygen species (ROS) levels and the ratio of apoptotic cells. Here, we compared the transcriptomes of untreated versus melatonin-treated PTr2 cells by RNA-seq analysis and found that differentially expressed genes (DEGs) were highly enriched in the Wnt signaling, TGF-beta signaling and mTOR signaling pathways. Moreover, pretreatment with MT upregulated the antioxidant-related genes such as early growth response3 (EGR3), WAP four-disulfide core domain1 (WFDC1), heme oxygenase1 (HMOX1) and vimentin (VIM). These findings reveal that melatonin protects PTr2 cells from H2O2-induced oxidative stress damage.