bluuebunny/crossref_metadata_2025 huggingface dataset:
Maternal Risk Factors for Stillbirth: A Registry–Based Study
Authors: Zile Irisa , Ebela Inguna , Rumba-Rozenfelde Ingrida
Published: July 2019

Background and Objectives: The number of stillbirths has reduced more slowly than has maternal mortality or mortality in children younger than 5 years, which were explicitly targeted in the Millennium Development Goals. Placental pathologies and infection associated with preterm birth are linked to a substantial proportion of stillbirths. Appropriate preconception care and quality antenatal care that is accessible to all women has the potential to reduce stillbirth rates. The aim of the present study was to assess potential risk factors associated with stillbirth within maternal medical diseases and obstetric complications. Materials and Methods: Retrospective cohort study (2001–2014) was used to analyse data from the Medical Birth Register on stillbirth and live births as controls. Adjusted Odds ratios (aOR) with 95% confidence intervals (CI) were estimated. Multiple regression model adjusted for maternal age, parity and gestational age. Results: The stillbirth rate was 6.2 per 1000 live and stillbirths. The presence of maternal medical diseases greatly increased the risk of stillbirth including diabetes mellitus (aOR = 2.5; p < 0.001), chronic hypertension 3.1 (aOR = 3.1; p < 0.001) and oligohydromnios/polyhydromnios (aOR = 2.4; p < 0.001). Pregnancy complications such as intrauterine growth restriction (aOR = 2.2; p < 0.001) was important risk factor for stillbirth. Abruption was associated with a 2.8 odds of stillbirth. Conclusions: Risk factors most significantly associated with stillbirth include maternal history of chronic hypertension and abruptio placenta which is a common cause of death in stillbirth. Early identification of potential risk factors and appropriate perinatal management are important issues in the prevention of adverse fetal outcomes and preventive strategies need to focus on improving antenatal detection of fetal growth restriction.
Analysis on Characteristics of ZnO Surface Acoustic Wave with and without Micro-Structures
Authors: Huang Huei-Yu , Chiang Hsi-Jen , Wu Ching-Zong , Lin Yi , Shen Yung-Kang
Published: July 2019

In this paper, we fabricate a surface acoustic wave (SAW) device with micro-structures on a zinc oxide (ZnO) thin film and measure its signal response. The manufacturing processes of the SAW device include the fabrication of micro-structures of a SAW element and its interdigital transducer by silicon micro-machining and the fabrication of a thin film of ZnO by RF magnetron sputtering. We, then, measure the SAW properties. This research investigates the properties of sputtered thin films for various amounts of O2/(Ar + O2) using Zn and ZnO targets. Regardless of target, the growth rate of the ZnO thin film decreases as the oxygen content increases. When the SAW is sputtered ZnO thin film using 30% oxygen, the digital signal of the SAW has better performance. The measurement signal of the SAW with micro-structures is similar to that without micro-structures.
Viability and Composition Validation of Commercial Probiotic Products by Selective Culturing Combined with Next-Generation Sequencing
Authors: Ullah Mati , Raza Ali , Ye Li , Yu Zhu
Published: July 2019

The consumption of dietary supplements to treat health complications or to improve overall health conditions has become a globally increasing trend that leads to the development of a large number of health-related novel products and expands the associated manufacturing industries around the world. In the current study, we applied selective culturing combined with next-generation sequencing to examine the microbial viability in terms of its culturability on culture medium, composition, and possible contamination in the selected 17 commercial probiotic products sold in the mainland China market. Additionally, the relative abundance of each individual bacterial content was also evaluated by using the generated sequencing reads. The tested probiotic product samples were subjected to Illumina HiSeq-2000 sequencing platform and thoroughly analyzed by the in-house developed bioinformatics pipeline. The comprehensive culturing and sequencing analysis revealed both viability and composition inaccuracy among the several tested probiotic products, however, no contaminant was identified during the analysis. Among the total, five probiotic products (29.41%) were found with an inaccurate or lower colony-forming unit (CFU) counts on culture media while four probiotic products (23.52%) have inaccurately labeled classification. This study provides an ideal qualitative and quantitative assessment approach, which can be used as a diagnostic tool for the accurate assessment of commercial probiotic supplements.
Flavonoids from Morus alba L. Leaves: Optimization of Extraction by Response Surface Methodology and Comprehensive Evaluation of Their Antioxidant, Antimicrobial, and Inhibition of α-Amylase Activities through Analytical Hierarchy Process
Authors: Cui Hao , Lu Tenghui , Wang Mingxin , Zou Xintong , Zhang Yang , Yang Xiudong , Dong Yao , Zhou Hongli
Published: July 2019

To explore the flavonoids from Morus alba L. leaves (MLF), the process of extracting was optimized by a response surface methodology and the antimicrobial and antioxidant activities were evaluated in vitro. The yield of flavonoids reached 50.52 mg g−1 under the optimized extraction conditions (i.e., extraction temperature, 70.85 °C; solvent concentration, 39.30%; extraction time, 120.18 min; and liquid/solid ratio, 34.60:1). The total flavonoids were extracted in organic solvents with various polarities, including petroleum ether (MLFp), ethyl acetate (MLFe), and n-butanol (MLFb). In vitro, the four MLF samples exhibited good antioxidant activities for scavenging of 2, 2′-azinobis-(3-ethylbenz-thiazoline-6-sulphonate) radical, 1, 1-diphenyl-2-picrylhydrazyl radical, and total reducing power. Regarding antimicrobial efficacy, the MLF samples suppressed the development of Staphylococcus aureus, Bacillus subtilis, and Bacillus pumilus. The MLF samples inhibited α-amylase activity to a certain extent. The analytical hierarchy process (AHP) was used to evaluate comprehensively the bioactivities of the MLF samples. The AHP results revealed that the bioactivity comprehensive score (78.83 μg mL−1) of MLFe was optimal among the four MLF samples. Morus alba L. leaves also exhibited non-hemolytic properties. All bioactivities suggested the potential of MLFe as a candidate resource in the food and drug industries.
Development and Validation of a Method for the Analysis of Bisoprolol and Atenolol in Human Bone
Authors: Fernandez-Lopez Lucia , Pellegrini Manuela , Rotolo Maria Concetta , Luna Aurelio , Falcon Maria , Mancini Rosanna
Published: July 2019

A method based on gas chromatography–mass spectrometry (GC–MS) is described for the determination of bisoprolol and atenolol in human bone. After the addition of lobivolol as internal standard, pulverized samples were incubated in acetonitrile for 1 h under ultrasounds. After adjusting the pH of the samples to 6, they were centrifuged, and the supernatants were subjected to solid phase extraction. Elution was achieved by using 3 mL of 2% ammonium hydroxide in 80:20 dichloromethane:isopropanol solution. Eluted samples were evaporated and derivatized. Chromatography was performed on a fused silica capillary column and analytes were determined in the selected-ion-monitoring (SIM) mode. The assay was validated in the range 0.1–0.3 ng/mg (depending on the drug) to 150 ng/mg, the mean absolute recoveries were 60% for bisoprolol and 106% for atenolol, the matrix effect was 69% for bisoprolol and 70% for atenolol and process efficiency was 41% for bisoprolol and 80% for atenolol. The intra- and inter-assay accuracy values were always better than 12%. The validated method was then applied to bone samples from two real forensic cases in which toxicological analysis in blood were positive for atenolol in the first case (0.65 µg/mL) and bisoprolol in the second case (0.06 µg/mL). Atenolol was found in bone samples from the corresponding case at the approximate concentration of 148 ng/mg and bisoprolol was found at 8 ng/mg.
Targeting Bacterial Biofilms by the Green Tea Polyphenol EGCG
Authors: Hengge Regine
Published: July 2019

Bacterial biofilms are multicellular aggregates in which cells are embedded in an extracellular matrix of self-produced biopolymers. Being refractory to antibiotic treatment and host immune systems, biofilms are involved in most chronic infections, and anti-biofilm agents are being searched for urgently. Epigallocatechin-3-gallate (EGCG) was recently shown to act against biofilms by strongly interfering with the assembly of amyloid fibres and the production of phosphoethanolamin-modified cellulose fibrils. Mechanistically, this includes a direct inhibition of the fibre assembly, but also triggers a cell envelope stress response that down-regulates the synthesis of these widely occurring biofilm matrix polymers. Based on its anti-amyloidogenic properties, EGCG seems useful against biofilms involved in cariogenesis or chronic wound infection. However, EGCG seems inefficient against or may even sometimes promote biofilms which rely on other types of matrix polymers, suggesting that searching for ‘magic bullet’ anti-biofilm agents is an unrealistic goal. Combining molecular and ecophysiological aspects in this review also illustrates why plants control the formation of biofilms on their surfaces by producing anti-amyloidogenic compounds such as EGCG. These agents are not only helpful in combating certain biofilms in chronic infections but even seem effective against the toxic amyloids associated with neuropathological diseases.
Selective Extraction of ω-3 Fatty Acids from Nannochloropsis sp. Using Supercritical CO2 Extraction
Authors: Leone Gian Paolo , Balducchi Roberto , Mehariya Sanjeet , Martino Maria , Larocca Vincenzo , Di Sanzo Giuseppe , Iovine Angela , Casella Patrizia , Marino Tiziana , Karatza Despina , Chianese Simeone , Musmarra Dino , Molino Antonio
Published: July 2019

In this article, microalgae Nannochloropsis sp. was used for fatty acid (FA) extraction, using a supercritical fluid-carbon dioxide (SF-CO2) extraction method. This study investigated the influence of different pre-treatment conditions by varying the grinding speed (200–600 rpm), pre-treatment time (2.5–10 min), and mixing ratio of diatomaceous earth (DE) and Nannochloropsis sp. biomass (0.5–2.0 DE/biomass) on FAs extraction. In addition, the effect of different operating conditions, such as pressure (100–550 bar), temperature (50–75 °C), and CO2 flow rate (7.24 and 14.48 g/min) on eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) recovery, was analyzed. Experimental data evidenced that, keeping constant the extraction conditions, the pre-treatment step enhanced the FAs extraction yield up to 3.4 fold, thereby the maximum extracted amount of FAs (61.19 mg/g) was attained with the pre-treatment with a ratio of DE/biomass of 1 at 600 rpm for 5 min. Moreover, by increasing both SF-CO2 pressure and temperature, the selectivity towards EPA was enhanced, while intermediate pressure and lower pressure promoted DHA recovery. The highest amount of extracted EPA, i.e., 5.69 mg/g, corresponding to 15.59%, was obtained at 75 °C and 550 bar with a CO2 flow rate of 14.48 g/min, while the maximum amount of extracted DHA, i.e., ~0.12 mg/g, equal to 79.63%, was registered at 50 °C and 400 bar with a CO2 flow rate of 14.48 g/min. Moreover, the increased CO2 flow rate from 7.24 to 14.48 g/min enhanced both EPA and DHA recovery.
Ionic Nanocomplexes of Hyaluronic Acid and Polyarginine to Form Solid Materials: A Green Methodology to Obtain Sponges with Biomedical Potential
Authors: Villamizar-Sarmiento María Gabriela , Moreno-Villoslada Ignacio , Martínez Samuel , Giacaman Annesi , Miranda Victor , Vidal Alejandra , Orellana Sandra L. , Concha Miguel , Pavicic Francisca , Lisoni Judit G. , Leyton Lisette , Oyarzun-Ampuero Felipe A.
Published: July 2019

We report on the design, development, characterization, and a preliminary cellular evaluation of a novel solid material. This material is composed of low-molecular-weight hyaluronic acid (LMWHA) and polyarginine (PArg), which generate aqueous ionic nanocomplexes (INC) that are then freeze-dried to create the final product. Different ratios of LMWHA/PArg were selected to elaborate INC, the size and zeta potential of which ranged from 100 to 200 nm and +25 to −43 mV, respectively. Turbidimetry and nanoparticle concentration analyses demonstrated the high capacity of the INC to interact with increasing concentrations of LMWHA, improving the yield of production of the nanostructures. Interestingly, once the selected formulations of INC were freeze-dried, only those comprising a larger excess of LMWHA could form reproducible sponge formulations, as seen with the naked eye. This optical behavior was consistent with the scanning transmission electron microscopy (STEM) images, which showed a tendency of the particles to agglomerate when an excess of LMWHA was present. Mechanical characterization evidenced low stiffness in the materials, attributed to the low density and high porosity. A preliminary cellular evaluation in a fibroblast cell line (RMF-EG) evidenced the concentration range where swollen formulations did not affect cell proliferation (93–464 µM) at 24, 48, or 72 h. Considering that the reproducible sponge formulations were elaborated following inexpensive and non-contaminant methods and comprised bioactive components, we postulate them with potential for biomedical purposes. Additionally, this systematic study provides important information to design reproducible porous solid materials using ionic nanocomplexes.
Investigation on the Selenization Treatment of Kesterite Cu2Mg0.2Zn0.8Sn(S,Se)4 Films for Solar Cell
Authors: Jiang Dongyue , Zhang Yu , Sui Yingrui , He Wenjie , Wang Zhanwu , Yang Lili , Wang Fengyou , Yao Bin
Published: July 2019

High-selenium Cu2Mg0.2Zn0.8Sn(S,Se)4 (CMZTSSe) films were prepared on a soda lime glass substrate using the sol–gel spin coating method, followed by selenization treatment. In this work, we investigated the effects of selenization temperature and selenization time on the crystal quality, and electrical and optical properties of CMZTSSe films. The study on the micro-structure by XRD, Raman, X-ray photoelectron spectroscopy (XPS), and energy-dispersive X-ray spectroscopy (EDS) analysis showed that all CMZTSSe samples had kesterite crystalline structure. In addition, the crystalline quality of CMZTSSe is improved and larger Se takes the site of S in CMZTSSe with the increase of selenization temperature and selenization time. When increasing the selenization temperature from 500 to 530 °C and increasing the annealing time from 10 to 15 min, the morphological studies showed that the microstructures of the films were dense and void-free. When further increasing the temperature and time, the crystalline quality of the films began to deteriorate. In addition, the bandgaps of CMZTSSe are tuned from 1.06 to 0.93 eV through adjusting the selenization conditions. When CMZTSSe samples are annealed at 530 °C for 15 min under Se atmosphere, the crystal quality and optical–electrical characteristics of CMZTSSe will be optimal, and the grain size and carrier concentration reach maximums of 1.5–2.5 μm and 6.47 × 1018 cm−3.
Phenol Abatement by Titanium Dioxide Photocatalysts: Effect of The Graphene Oxide Loading
Authors: Naknikham Usuma , Magnacca Giuliana , Qiao Ang , Kristensen Peter Kjær , Boffa Vittorio , Yue Yuanzheng
Published: July 2019

Hetero-photocatalytic graphene-TiO2 materials have, in the literature, been found to possess better photocatalytic activity for environmental applications compared to pure TiO2. These types of materials can be prepared in different ways; however, their photocatalytic performance and quality are not easily controlled and reproduced. Therefore, we synthetized graphene oxide-TiO2 nanoparticles by sol-gel reaction from TiCl4, as precursor, with two different methods of synthesis and with a graphene oxide (GO) loading ranging from 0 to 1.0. This approach led to a good adhesion of GO to TiO2 through the Ti-O-C bonding, which could enhance the photocatalytic performances of the materials. Overall, 0.05 wt % GO loading gave the highest rate in the photodegradation of phenol under visible light, while higher GO loadings had a negative impact on the photocatalytic performances of the composites. The 0.05 wt % GO-TiO2 composite material was confirmed to be a promising photocatalyst for water pollutant abatement. The designed synthetic approach could easily be implemented in large-scale production of the GO-TiO2 coupling materials.
Formation and Stability of Pea Proteins Nanoparticles Using Ethanol-Induced Desolvation
Authors: Doan Chi Diem , Ghosh Supratim
Published: July 2019

Protein nanoparticles have recently found a lot of interests due to their unique physicochemical properties and structure-functionality compared to the conventional proteins. The aim of this research was to synthesize pea protein nanoparticles (PPN) using ethanol-induced desolvation, to determine the changes in secondary structures and the particle stability in an aqueous dispersion. The nanoparticles were prepared by diluting 3.0 wt% pea protein solutions in 1–5 times ethanol at pH 3 and 10 at different temperatures. Higher ratios of ethanol caused greater extent of desolvation and larger sizes of PPN. After homogenization at 5000 psi for 5 min, PPN displayed uniform size distribution with a smaller size and higher zeta potential at pH 10 compared to pH 3. PPN prepared from a preliminary thermal treatment at 95 °C revealed a smaller size than those synthesized at 25 °C. Electron microscopy showed roughly spherical shape and extensively aggregated state of the nanoparticles. Addition of ethanol caused a reduction in β-sheets and an increase in α-helices and random coil structures of the proteins. When PPN were separated from ethanol and re-dispersed in deionized water (pH 7), they were stable over four weeks, although some solubilization of proteins leading to a loss in particle size was observed.
Editorial for the Special Issue on “Nucleic Acid Architectures for Therapeutics, Diagnostics, Devices and Materials”
Authors: Halman Justin R. , Afonin Kirill A.
Published: July 2019

The use of nucleic acids (RNA and DNA) offers a unique and multifunctional platform for numerous applications including therapeutics, diagnostics, nanodevices, and materials [...]
Electrospun Polyvinylidene Fluoride-Based Fibrous Scaffolds with Piezoelectric Characteristics for Bone and Neural Tissue Engineering
Authors: Li Yuchao , Liao Chengzhu , Tjong Sie Chin
Published: July 2019

Polyvinylidene fluoride (PVDF) and polyvinylidene fluoride-trifluoroethylene (P(VDF-TrFE) with excellent piezoelectricity and good biocompatibility are attractive materials for making functional scaffolds for bone and neural tissue engineering applications. Electrospun PVDF and P(VDF-TrFE) scaffolds can produce electrical charges during mechanical deformation, which can provide necessary stimulation for repairing bone defects and damaged nerve cells. As such, these fibrous mats promote the adhesion, proliferation and differentiation of bone and neural cells on their surfaces. Furthermore, aligned PVDF and P(VDF-TrFE) fibrous mats can enhance neurite growth along the fiber orientation direction. These beneficial effects derive from the formation of electroactive, polar β-phase having piezoelectric properties. Polar β-phase can be induced in the PVDF fibers as a result of the polymer jet stretching and electrical poling during electrospinning. Moreover, the incorporation of TrFE monomer into PVDF can stabilize the β-phase without mechanical stretching or electrical poling. The main drawbacks of electrospinning process for making piezoelectric PVDF-based scaffolds are their small pore sizes and the use of highly toxic organic solvents. The small pore sizes prevent the infiltration of bone and neuronal cells into the scaffolds, leading to the formation of a single cell layer on the scaffold surfaces. Accordingly, modified electrospinning methods such as melt-electrospinning and near-field electrospinning have been explored by the researchers to tackle this issue. This article reviews recent development strategies, achievements and major challenges of electrospun PVDF and P(VDF-TrFE) scaffolds for tissue engineering applications.
Facile Preparation of Cu2O Nanoparticles and Reduced Graphene Oxide Nanocomposite for Electrochemical Sensing of Rhodamine B
Authors: He Quanguo , Liu Jun , Tian Yaling , Wu Yiyong , Magesa Felista , Deng Peihong , Li Guangli
Published: July 2019

In this paper, the preparation, characterization, and electrochemical application of Cu2O nanoparticles and an electrochemical reduced graphene oxide nanohybrid modified glassy carbon electrode (denoted as Cu2O NPs‒ERGO/GCE) are described. This modified electrode was used as an electrochemical sensor for the catalytic oxidation of rhodamine B (RhB), and it exhibited an excellent electrochemical performance for RhB. The oxidation potential of RhB was decreased greatly, and the sensitivity to detect RhB was improved significantly. Under optimum conditions, a linear dynamic range of 0.01–20.0 μM and a low detection limit of 0.006 μM were obtained with the Cu2O NPs‒ERGO/GCE by using second‒order derivative linear sweep voltammetry. In addition, the selectivity of the prepared modified electrode was analyzed for the determination of RhB. The practical application of this sensor was investigated for the determination of RhB in food samples, and satisfactory results were obtained.
Plasmonic Colour Printing by Light Trapping in Two-Metal Nanostructures
Authors: Wilson Keith , Marocico Cristian A. , Pedrueza-Villalmanzo Esteban , Smith Christopher , Hrelescu Calin , Bradley A. Louise
Published: July 2019

Structural colour generation by nanoscale plasmonic structures is of major interest for non-bleaching colour printing, anti-counterfeit measures and decoration applications. We explore the physics of a two-metal plasmonic nanostructure consisting of metallic nanodiscs separated from a metallic back-reflector by a uniform thin polymer film and investigate the potential for vibrant structural colour in reflection. We demonstrate that light trapping within the nanostructures is the primary mechanism for colour generation. The use of planar back-reflector and polymer layers allows for less complex fabrication requirements and robust structures, but most significantly allows for the easy incorporation of two different metals for the back-reflector and the nanodiscs. The simplicity of the structure is also suitable for scalability. Combinations of gold, silver, aluminium and copper are considered, with wide colour gamuts observed as a function of the polymer layer thickness. The structural colours are also shown to be insensitive to the viewing angle. Structures of copper nanodiscs with an aluminium back-reflector produce the widest colour gamut.
Ultrafast Growth of Uniform Multi-Layer Graphene Films Directly on Silicon Dioxide Substrates
Authors: Zhou Lijie , Wei Shuai , Ge Chuanyang , Zhao Chao , Guo Bin , Zhang Jia , Zhao Jie
Published: July 2019

To realize the applications of graphene in electronics, a large-scale, high-quality, and uniform graphene film should first be placed on the dielectric substrates. Challenges still remain with respect to the current methods for the synthesis graphene directly on the dielectric substrates via chemical vapor deposition, such as a low growth rate and poor quality. Herein, we present an ultrafast method for direct growth of uniform graphene on a silicon dioxide (SiO2/Si) substrate using methanol as the only carbon source. A 1 × 1 cm2 SiO2/Si substrate square was almost fully covered with graphene within 5 min, resulting in a record growth rate of ~33.6 µm/s. This outcome is attributed to the quick pyrolysis of methanol, with the help of trace copper atoms. The as-grown graphene exhibited a highly uniform thickness, with a sheet resistance of 0.9–1.2 kΩ/sq and a hole mobility of up to 115.4 cm2/V·s in air at room temperature. It would be quite suitable for transparent conductive electrodes in electrophoretic displays and may be interesting for related industrial applications.
Impact of Arginine Nutrition and Metabolism during Pregnancy on Offspring Outcomes
Authors: Hsu Chien-Ning , Tain You-Lin
Published: July 2019

By serving as a precursor for the synthesis of nitric oxide, polyamines, and other molecules with biological importance, arginine plays a key role in pregnancy and fetal development. Arginine supplementation is a potential therapy for treating many human diseases. An impaired arginine metabolic pathway during gestation might produce long-term morphological or functional changes in the offspring, namely, developmental programming to increase vulnerability to developing a variety of non-communicable diseases (NCDs) in later life. In contrast, reprogramming is a strategy that shifts therapeutic interventions from adulthood to early-life, in order to reverse the programming processes, which might counterbalance the rising epidemic of NCDs. This review presented the role of arginine synthesis and metabolism in pregnancy. We also provided evidence for the links between an impaired arginine metabolic pathway and the pathogenesis of compromised pregnancy and fetal programming. This was followed by reprogramming strategies targeting the arginine metabolic pathway, to prevent the developmental programming of NCDs. Despite emerging evidence from experimental studies showing that targeting the arginine metabolic pathway has promise as a reprogramming strategy in pregnancy to prevent NCDs in the offspring, these results need further clinical application.
Relative Validity of a Food and Beverage Preference Questionnaire to Characterize Taste Phenotypes in Children Adolescents and Adults
Authors: Jilani Hannah , Pohlabeln Hermann , De Henauw Stefaan , Eiben Gabriele , Hunsberger Monica , Molnar Dénes , Moreno Luis , Pala Valeria , Russo Paola , Solea Antonia , Veidebaum Toomas , Ahrens Wolfgang , Hebestreit Antje ,   on behalf of the IDEFICS and I.Family consortia
Published: July 2019

To assess the relative validity of our food and beverage preference questionnaire we investigated the association between sweet and fatty taste preference scores (assessed using a food and beverage preference questionnaire) and sweet and fatty food propensity scores (derived from a food frequency questionnaire). In I.Family, a large European multi-country cohort study, 12,207 participants from Cyprus, Estonia, Germany, Hungary, Italy, Spain and Sweden, including 5291 adults, 3082 adolescents, and 3834 children, completed a food and beverage preference questionnaire with 63 items. Cumulative preference scores for sweet and fatty taste were calculated from the single item ranking ranging from 1 to 5. The relative consumption frequency of foods classified as sweet and fatty was used to calculate the corresponding consumption propensities, a continuous variable ranging from 0 to 100. We conducted regression analyses to investigate the association between sweet and fatty taste preference scores and sweet and fatty food propensity scores, respectively, separately for adults, adolescents ≥12 years, and for children <12 years. The overall sweet taste preference score was positively associated with the sweet food consumption propensity score (β = 2.4, 95% CI: 2.1;2.7) and the fatty taste preference score was positively associated with the fatty food consumption propensity score (β = 2.0, 95% CI: 1.8;2.2). After stratification for age (children <12 years, adolescents ≥12 years, and adults), the effect remained significant in all age groups and was strongest in adolescents and adults. We conclude that our food and beverage preference questionnaire is a useful instrument for epidemiological studies on sensory perception and health outcomes and for the characterization of sensory taste phenotypes.
Investigation of Nicotianamine and 2′ Deoxymugineic Acid as Enhancers of Iron Bioavailability in Caco-2 Cells
Authors: Beasley Jesse T. , Hart Jonathan J. , Tako Elad , Glahn Raymond P. , Johnson Alexander A. T.
Published: July 2019

Nicotianamine (NA) is a low-molecular weight metal chelator in plants with high affinity for ferrous iron (Fe2+) and other divalent metal cations. In graminaceous plant species, NA serves as the biosynthetic precursor to 2′ deoxymugineic acid (DMA), a root-secreted mugineic acid family phytosiderophore that chelates ferric iron (Fe3+) in the rhizosphere for subsequent uptake by the plant. Previous studies have flagged NA and/or DMA as enhancers of Fe bioavailability in cereal grain although the extent of this promotion has not been quantified. In this study, we utilized the Caco-2 cell system to compare NA and DMA to two known enhancers of Fe bioavailability—epicatechin (Epi) and ascorbic acid (AsA)—and found that both NA and DMA are stronger enhancers of Fe bioavailability than Epi, and NA is a stronger enhancer of Fe bioavailability than AsA. Furthermore, NA reversed Fe uptake inhibition by Myricetin (Myr) more than Epi, highlighting NA as an important target for biofortification strategies aimed at improving Fe bioavailability in staple plant foods.
Rice Porridge Containing Welsh Onion Root Water Extract Alleviates Osteoarthritis-Related Pain Behaviors, Glucose Levels, and Bone Metabolism in Osteoarthritis-Induced Ovariectomized Rats
Authors: Yang Hye Jeong , Kim Min Jung , Qiu Jing Yi , Zhang Ting , Wu Xuangao , Jang Dai-Ja , Park Sunmin
Published: July 2019

Rice porridge containing Allium fistulosum (Welsh onion) root water extract (RAFR) has anti-inflammatory bioactive compounds. We examined whether the long-term administration of rice porridge with RAFR would prevent or delay the progression of osteoarthritis and menopausal symptoms in estrogen-deficient animals by ovariectomy. The rats consumed 40% fat energy diets containing 250 mg RAFR (rice: Allium fistulosum root = 13:1)/kg body weight (bw) (OVX-OA-RAFR-Low), 750 mg RAFR/kg bw (OVX-OA-RAFR-High) and 750 mg starch and protein/kg bw(OVX), respectively. After consuming the assigned diets for eight weeks, monoiodoacetate (OVX-OA) or saline (OVX) were injected into the knee joints of the rats for an additional three weeks. Sham rats were administered saline injections (normal-control). OVX-OA-RAFR improved oral glucose tolerance and also protected against decreases in bone mineral density and lean body mass in the legs and increases in fat mass in the abdomen, compared to the OVX and OVX-OA. OVX-OA-RAFR improved swelling and limping scores, normalized weight distribution between the osteoarthritic and normal limbs, and increased maximum running speeds compared to the OVX-OA. The OVX-OA deteriorated the articular cartilage by reducing the articular matrix and bone loss in the knee joint and it prevented knee joint deterioration when compared to the OVX. The improvement in osteoarthritis symptoms in OVX-OA-RAFR decreased the mRNA expression of matrix metallo-proteinase-1 and matrix metalloproteinase-13, tumor necrosis factor-α, and interleukin-1β and interleukin-6 in the articular cartilage compared to OVX-OA rats. In conclusions, RAFR is effective in treating osteoarthritis symptoms and it may be used for a therapeutic agent in osteoarthritis-induced menopausal women.
Antiviral Agents in Development for Zika Virus Infections
Authors: Baz Mariana , Boivin Guy
Published: July 2019

In 1947, Zika virus (ZIKV), a mosquito-borne flavivirus was identified in Uganda and subsequently spread to Asia and the Pacific regions. In 2015, it was introduced in Brazil causing an important social and sanitary alarm due to its increased virulence and rapid dissemination. Importantly, ZIKV infections have been associated with severe neurological complications such as Guillain–Barré syndrome and microcephaly in fetuses and newborns. Although enormous efforts were made by investigators in the development of effective countermeasures against ZIKV, there is still no approved specific antiviral drug for the treatment of ZIKV infections. Herein, we review several anti ZIKV candidates including drugs targeting both the virus (structural proteins and enzymes) and cellular elements.
The Investigation of Silicon Localization and Accumulation in Citrus
Authors: Mvondo-She Mireille Asanzi , Marais Diana
Published: July 2019

Several studies have demonstrated Si absorption in monocotyledon and dicotyledon species. Regarding Si accumulation, studies in monocotyledons have identified Si deposition around the cell wall, cuticle layer bulliform cells, silica cells and endodermal cells. In previous studies with different citrus species there were evidence of Si accumulation, however no information on Si deposition can be found. Therefore, in this study, Si was applied (0 and 1000 mg L−1) to the roots of two citrus species, ‘Delta’ Valencia (Citrus sinensis) and ‘Nules’ Clementine (Citrus reticulata). Si accumulation were investigated in new, and old leaves and roots. Si deposition was investigated through scanning electron microscopy using energy dispersive analysis X-ray, environmental scanning electron microscopy and light microscopy. Si accumulation was significantly higher in Si treated leaves comparatively to the control in both young and mature leaves. Meanwhile, Si accumulation increased with leaf age. Additionally, Si accumulation was significantly higher in roots than in leaves. With respect to Si deposition, granules were identified in the epidermal cells through SEM and ESEM studies. The light microscopy identified the presence of Si granules in the surface and around the outer cell surface forming the cuticle-silica double layer of the lower epidermis in Si treated plants. Silica deposit were not found on the abaxial leaf surface. The findings suggest a passive uptake in citrus species.
Influence of Surface Modified Nanodiamonds on Dielectric and Mechanical Properties of Silicone Composites
Authors: Shakun Alexandra , Anyszka Rafal , Sarlin Essi , Blume Anke , Vuorinen Jyrki
Published: July 2019

Detonation nanodiamonds, also known as ultradispersed diamonds, possess versatile chemically active surfaces, which can be adjusted to improve their interaction with elastomers. Such improvements can result in decreased dielectric and viscous losses of the composites without compromising other in-rubber properties, thus making the composites suitable for new demanding applications, such as energy harvesting. However, in most cases, surface modification of nanodiamonds requires the use of strong chemicals and high temperatures. The present study offers a less time-consuming functionalization method at 40 °C via reaction between the epoxy-rings of the modifier and carboxylic groups at the nanodiamond surface. This allows decorating the nanodiamond surface with chemical groups that are able to participate in the crosslinking reaction, thus creating strong interaction between filler and elastomer. Addition of 0.1 phr (parts per hundred rubber) of modified nanodiamonds into the silicone matrix results in about fivefold decreased electric losses at 1 Hz due to a reduced conductivity. Moreover, the mechanical hysteresis loss is reduced more than 50% and dynamic loss tangent at ambient temperature is lowered. Therefore, such materials are recommended for the dielectric energy harvesting application, and they are expected to increase its efficiency.
Janus Nanostructures from ABC/B Triblock Terpolymer Blends
Authors: Steinhaus Andrea , Srivastva Deepika , Nikoubashman Arash , Gröschel André H.
Published: July 2019

Lamella-forming ABC triblock terpolymers are convenient building blocks for the synthesis of soft Janus nanoparticles (JNPs) by crosslinking the B domain that is “sandwiched” between A and C lamellae. Despite thorough synthetic variation of the B fraction to control the geometry of the sandwiched microphase, so far only Janus spheres, cylinders, and sheets have been obtained. In this combined theoretical and experimental work, we show that the blending of polybutadiene homopolymer (hPB) into lamella morphologies of polystyrene-block-polybutadiene-block-polymethylmethacrylate (SBM) triblock terpolymers allows the continuous tuning of the polybutadiene (PB) microphase. We systematically vary the volume fraction of hPB in the system, and we find in both experiments and simulations morphological transitions from PB-cylinders to perforated PB-lamellae and further to continuous PB-lamellae. Our simulations show that the hPB is distributed homogeneously in the PB microdomains. Through crosslinking of the PB domain and redispersion in a common solvent for all blocks, we separate the bulk morphologies into Janus cylinders, perforated Janus sheets, and Janus sheets. These studies suggest that more complex Janus nanostructures could be generated from ABC triblock terpolymers than previously expected.
A New Stream of Spiritual Literature: Bei Cun’s The Baptizing River
Authors: Starr Chloë
Published: July 2019

This essay traces the emergence of new categories of “spiritual writing” in Chinese literature, before offering an interpretation of Bei Cun’s 1992 novel The Baptizing River (Shixi de he 施洗的河) as an exemplar. Bei Cun’s first novel as a “Christian author” attracted much critical attention, given the contrast with the author’s prior works and its message of spiritual salvation at a time of political change and metaphysical searching. A psychosocial biography of its anti-hero Liu Lang, set in wartime China, the novel charts the protagonist’s criminal livelihood, descent into moral depravity, and gradual questioning of life and purpose. This essay foregrounds the structure of the novel and explores how narrative form and theological meaning interact. To do this, it traces the course of the river journeys that mark the different stages of Liu Lang’s life and which culminate in his unorthodox baptismal rebirth.
Determining the Value of Soil Inorganic Carbon Stocks in the Contiguous United States Based on the Avoided Social Cost of Carbon Emissions
Authors: Groshans Garth R. , Mikhailova Elena A. , Post Christopher J. , Schlautman Mark A. , Zhang Lisha
Published: July 2019

Carbon sequestered as soil inorganic carbon (SIC) provides a regulating ecosystem service, which can be assigned a monetary value based on the avoided social cost of carbon (SC-CO2). By definition, the SC-CO2 is a measure, in dollars, of the long-term damage resulting from the emission of a metric ton of carbon dioxide (CO2). Therefore, this dollar figure also represents the value of damages avoided due to an equivalent reduction or sequestration of CO2. The objective of this study was to assess the value of SIC stocks in the contiguous United States (U.S.) by soil order, soil depth (0–20, 20–100, 100–200 cm), land resource region (LRR), state, and region using information from the State Soil Geographic (STATSGO) database together with a reported SC-CO2 of $42 (U.S. dollars). With this approach, the calculated monetary value for total SIC storage in the contiguous U.S. was between $3.48T (i.e., $3.48 trillion U.S. dollars, where T = trillion = 1012) and $14.4T, with a midpoint value of $8.34T. Soil orders with the highest (midpoint) values for SIC storage were: 1) Mollisols ($3.57T), 2) Aridisols ($1.99T), and 3) Alfisols ($841B) (i.e., $841B is 841 billion U.S. dollars, where B = billion = 109). When normalized by land area, the soil orders with the highest (midpoint) values for SIC storage were: 1) Vertisols ($3.57 m−2), 2) Aridisols ($2.45 m−2), and 3) Mollisols ($1.77 m−2). Most of the SIC value was associated with the 100–200 cm depth interval, with a midpoint value of $4T and an area-normalized value of $0.54 m−2. The LRRs with the highest (midpoint) values of SIC storage were: 1) D—Western Range and Irrigated Region ($1.77T), 2) H—Central Great Plains Winter Wheat and Range Region ($1.49T), and 3) M—Central Feed Grains and Livestock Region ($1.02T). When normalized by land area, the LRRs were ranked: 1) I—Southwest Plateaus and Plains Range and Cotton Region ($5.36 m−2), 2) J—Southwestern Prairies Cotton and Forage Region ($4.56 m−2), and 3) H—Central Great Plains Winter Wheat and Range Region ($2.56 m−2). States with the highest (midpoint) values for SIC storage were: 1) Texas ($2.96T), 2) New Mexico ($572B), and 3) Montana ($524B). When normalized by land area, the states were ranked: 1) Texas ($4.47 m−2), 2) Utah ($2.77 m−2), and 3) Minnesota ($2.17 m−2). Lastly, regions with the highest (midpoint) values for SIC storage were: 1) South Central ($3.13T), 2) West ($1.98T), and 3) Northern Plains ($1.62T). When normalized by land area, the regions were ranked: 1) South Central ($2.90 m−2), 2) Midwest ($1.32 m−2), and 3) West ($1.02 m−2). Results from this study demonstrate a new approach for assigning monetary values to SIC stocks at various scales based on their role in providing ecosystem services for climate regulation and carbon sequestration.
Key Enablers of and Barriers to the Uptake and Implementation of Nature-Based Solutions in Urban Settings: A Review
Authors: Ershad Sarabi Shahryar , Han Qi , L. Romme A. Georges , de Vries Bauke , Wendling Laura
Published: July 2019

Climate change and urbanization have resulted in several societal challenges for urban areas. Nature-based solutions (NBS) have been positioned as solutions for enhancing urban resilience in the face of these challenges. However, the body of conceptual and practical knowledge regarding NBS remains fragmented. This study addresses this gap by means of a systematic review of the literature, to define NBS as a theoretical concept; its broader significance with respect to societal challenges; the key stakeholders in NBS planning, implementation and management; and major barriers to and enablers of NBS uptake. The results of this review reveal that, despite a lack of consensus about the definition of NBS, there is a shared understanding that the NBS concept encompasses human and ecological benefits beyond the core objective of ecosystem conservation, restoration or enhancement. Significant barriers to and enablers of NBS are discussed, along with a proposed strategic planning framework for successful uptake of NBS.
An Uncertainty Descriptor for Quantitative Measurement of the Uncertainty of Remote Sensing Images
Authors: Zhang Qi , Zhang Penglin
Published: July 2019

Reliable image classification results are crucial for the application of remote sensing images, but the reliability of image classification has received less attention. In particular, the inherent uncertainty of remote sensing images has been disregarded. The uncertainty of a remote sensing image accumulates and propagates continuously in the classification process and ultimately affects the reliability of the classification results. Therefore, quantitative description and investigation of the inherent uncertainty of remote sensing images are crucial in achieving reliable remote sensing image classification. In this study, we analyze the sources of uncertainty of remote sensing images in detail and propose a quantitative descriptor for measuring image uncertainty comprehensively and effectively. In addition, we also design two verification schemes to verify the validity of the proposed uncertainty descriptor. Finally, the validity of the proposed uncertainty descriptor is confirmed by experimental results on three real remote sensing images. Our study on the uncertainty of remote sensing images may help the development of uncertainty control methods and reliable classification schemes of remote sensing images.
Research on Adaptive Multi-Source Information Fault-Tolerant Navigation Method Based on No-Reference System Diagnosis
Authors: Zhang Ling , Cui Yuchen , Xiong Zhi , Liu Jianye , Lai Jizhou , Lv Pin
Published: July 2019

In order to obtain accurate and optimized navigation sensor information, it is necessary to study information fusion and fault diagnosis with high reliability, high precision and high autonomy, and then to propose a rapid and accurate intelligent decision-making scheme based on multi-source and heterogeneous navigation information. In view of the existing fault-tolerant navigation federated filter structure, the method of assuming the reference system (inertial navigation system) to be fault-free and then diagnosing the measuring sensor fault is generally adopted. Considering that the structure of the filter can’t detect and isolate the faults of the inertial navigation system, the performance of the MEMS inertial navigation system declines due to complex environments resulting from vibrations and temperature changes; additionally, external interference may lead to the direct failure of the MEMS inertial device. Therefore, this paper studies a fault-tolerant navigation method based on a no-reference system. For the sensor sub-system of a custom micro air vehicle (MAV), a fault detection method based on a reference-free system is proposed. Based on the fault type analysis, some improvements have been made to the existing residual chi-square detection method, and an interactive residual fault detection method with distributed states is proposed. On this basis, aiming at the characteristics of a reference-free system, the weight distribution scheme of the reference system and the tested systems are studied, and a self-regulation filter fusion and fault detection method based on reference-free system is designed.
SINS/Landmark Integrated Navigation Based on Landmark Attitude Determination
Authors: Xu Shuqing , Zhou Haiyin , Wang Jiongqi , He Zhangming , Wang Dayi
Published: July 2019

Based on the situation that the traditional SINS (strapdown inertial navigation system)/CNS (celestial navigation system) integrated navigation system fails to realize all-day and all-weather navigation, this paper proposes a SINS/Landmark integrated navigation method based on landmark attitude determination to solve this problem. This integrated navigation system takes SINS as the basic scheme and uses landmark navigation to correct the error of SINS. The way of the attitude determination is to use the landmark information photographed by the landmark camera to complete feature matching. The principle of the landmark navigation and the process of attitude determination are discussed, and the feasibility of landmark attitude determination is analyzed, including the orthogonality of the attitude transform matrix, as well as the influences of the factors such as quantity and geometric position of landmarks. On this basis, the paper constructs the equations of the SINS/Landmark integrated navigation system, testifies the effectiveness of landmark attitude determination on the integrated navigation by Kalman filter, and improves the navigation precision of the system.
Solidarity Economy, Social Enterprise, and Innovation Discourses: Understanding Hybrid Forms in Postcolonial Colombia
Authors: Calvo Martínez Sara , Morales Pachón Andrés , Martín Martín José María , Molina Moreno Valentín
Published: July 2019

Dominant conceptions of solidarity economy, social enterprise, and innovation (SSEI) remain poorly positioned for understanding the diverse models emerging across the global South. The purpose of this paper is to examine the power relations between the global North and South in the production and dissemination of SSEI knowledge, highlighting the importance of recognizing alternative discourses in the global South. This contextual analysis is developed through consideration of the construction of the hybrid SSEI model in Colombia, drawing upon postcolonial theory and using Nicholls’ framework on the legitimacy of SSEI discourses. This paper offers the first application of postcolonial theory to the analysis of SSEI in the global South. This research has demonstrated that the construction of the SSEI sector in Colombia is a reflection of the dynamic interplay of the hybrids, as it incorporates the hero entrepreneur and business-like discourses within the traditional community discourse, which indeed is a combination of domestic (indigenous collective practices) and colonizer influences (e.g., cooperatives, associations). This paper also identifies the current tensions that have emerged from such hybridity within the country.