bluuebunny/crossref_metadata_2025 huggingface dataset:
Plasmonics for advance single-molecule fluorescence spectroscopy and imaging in biology
Authors: Zaza Cecilia , Simoncelli Sabrina
Published: August 2022

The elucidation of complex biological processes often requires monitoring the dynamics and spatial organization of multiple distinct proteins organized on the sub-micron scale. This length scale is well below the diffraction limit of light, and as such not accessible by classical optical techniques. Further, the high molecular concentrations found in living cells, typically in the micro- to mili-molar range, preclude single-molecule detection in confocal volumes, essential to quantify affinity constants and protein-protein reaction rates in their physiological environment. To push the boundaries of the current state of the art in single-molecule fluorescence imaging and spectroscopy, plasmonic materials offer encouraging perspectives. From thin metallic films to complex nano-antenna structures, the near-field electromagnetic coupling between the electronic transitions of single emitters and plasmon resonances can be exploited to expand the toolbox of single-molecule based fluorescence imaging and spectroscopy approaches. Here, we review two of the most current and promising approaches to study biological processes with unattainable level of detail. On one side, we discuss how the reduction of the fluorescence lifetime of a molecule as it approaches a thin metallic film can be exploited to decode axial information with nanoscale precision. On the other, we review how the tremendous progress on the design of plasmonic antennas that can amplify and confine optical fields at the nanoscale, powered a revolution in fluorescence correlation spectroscopy. Besides method development, we also focus in describing the most interesting biological application of both technologies.
The effects of genetic distance, nutrient conditions, and recognition ways on outcomes of kin recognition in Glechoma longituba
Authors: Fan Yilei , Zhang Ruichang , Zhang Yuanlin , Yue Ming
Published: August 2022

Kin recognition might help plants decrease competitive cost and improve inclusive fitness with close genes; thus it might interact with environmental factors to affect communities. Whether and how various factors, such as the genetic distance of neighbors, environmental stressors, or the way a plant recognizes its neighbors, might modify plant growth strategies remains unclear. To answer these questions, we conducted experiments in which ramets of a clonal plant, Glechoma longituba, were grown adjacent to different genetically related neighbors (clone kin / close kin / distant kin) in different nutrient conditions (high / medium / low), or with only root exudates from pre-treatment in culture solution. By comparing competitive traits, we found that: (1) kin recognition in G. longituba was enhanced with closer genetic distance; (2) the outcomes of kin recognition were influenced by the extent of nutrient shortage; (3) kin recognition helped to alleviate the nutrient shortage effect; (4) kin recognition via root exudates affected only below-ground growth. Our results provide new insights on the potential for manipulating the outcome of kin recognition by altering neighbor genetic distance, nutrient conditions and recognition ways. Moreover, kin recognition can help plants mitigate the effects of nutrient shortage, with potential implications in agricultural research.
Individual and combinatorial effects of SNP and NaHS on morpho-physio-biochemical attributes and phytoextraction of chromium through Cr-stressed spinach (Spinacia oleracea L.)
Authors: Ma Jing , Saleem Muhammad Hamzah , Yasin Ghulam , Mumtaz Sahar , Qureshi Freeha Fatima , Ali Baber , Ercisli Sezai , Alhag Sadeq K. , Ahmed Ahmed Ezzat , Vodnar Dan C. , Hussain Iqbal , Marc Romina Alina , Chen Fu
Published: August 2022

Chromium (Cr) is a toxic heavy metal that contaminates soil and water resources after its discharge from different industries. A pot experiment was conducted to determine the effects of single and/or combined application of sodium nitroprusside (SNP) (250 μM) and sodium hydrogen sulfide (NaHS) (1 mM) on growth, photosynthetic pigments, gas exchange characteristics, oxidative stress biomarkers, antioxidant machinery (enzymatic and non-enzymatic antioxidants), ion uptake, organic acid exudation, and Cr uptake of spinach (Spinacia oleracea L.) exposed to severe Cr stress [Cr: 0 (no Cr), 150, and 300 μM]. Our results depicted that Cr addition to the soil significantly (P < 0.05) decreased plant growth and biomass, gas exchange attributes, and mineral uptake by S. oleracea when compared to the plants grown without the addition of Cr. However, Cr toxicity boosted the production of reactive oxygen species (ROS) by increasing the content of malondialdehyde (MDA), which is the indication of oxidative stress in S. oleracea, and was also manifested by hydrogen peroxide (H2O2) content and electrolyte leakage to the membrane-bound organelles. The results showed that the activities of various antioxidative enzymes, such as superoxidase dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and the content of non-enzymatic antioxidants, such as phenolic, flavonoid, ascorbic acid, and anthocyanin, initially increased with an increase in the Cr concentration in the soil. The results also revealed that the levels of soluble sugar, reducing sugar, and non-reducing sugar were decreased in plants grown under elevating Cr levels, but the accumulation of the metal in the roots and shoots of S. oleracea, was found to be increased, and the values of bioaccumulation factor were <1 in all the Cr treatments. The negative impacts of Cr injury were reduced by the application of SNP and NaHS (individually or combined), which increased plant growth and biomass, improved photosynthetic apparatus, antioxidant enzymes, and mineral uptake, as well as diminished the exudation of organic acids and oxidative stress indicators in roots of S. oleracea by decreasing Cr toxicity. Here, we conclude that the application of SNP and NaHS under the exposure to Cr stress significantly improved plant growth and biomass, photosynthetic pigments, and gas exchange characteristics; regulated antioxidant defense system and essential nutrient uptake; and balanced organic acid exudation pattern in S. oleracea.
Tipping point of plant functional traits of Leymus chinensis to nitrogen addition in a temperate grassland
Authors: Yang Guojiao , Zhang Zijia , Zhang Guangming , Liu Qianguang , Zheng Peiming , Wang Renqing
Published: August 2022

It has widely been documented that nitrogen (N) enrichment stimulates plant growth and modifies plant functional traits in the terrestrial ecosystem. However, it remains unclear whether there are critical transitions or tipping points for the response of plant growth or traits to N enrichment, and how these responses differ to different N forms. We chose the native, perennial clonal grass, Leymus chinensis in Inner Mongolia steppe, and conducted a field experiment, in which six N addition rates (0, 2, 5, 10, 20, and 50 g N m–2 year–1) and five N compound types [NH4NO3, (NH4)2SO4, NH4HCO3, CO(NH2)2, slow-release CO(NH2)2] are considered. Here, we found that the different N compound types had no significant effect on the growth of L. chinensis. N addition rate significantly increased plant aboveground biomass and leaf nitrogen concentration, whereas decreased leaf dry matter content. The tipping point for N-induced aboveground biomass increase was at 10 g N m–2 year–1, and the changes in functional traits were at N addition rates of 20 g N m–2 year–1. Our findings suggested that the responses of aboveground biomass and functional traits to N addition were asymmetric, in which responses in aboveground biomass were more sensitive than that in functional traits. The differential sensitivity of aboveground biomass and functional traits of L. chinensis occurred to N deposition highlights the importance of functional traits in mediating ecosystem functioning in the face of N deposition, regardless of which chemical forms dominate in the deposited N.
Risk perception, but also political orientation, modulate behavioral response to COVID-19: A randomized survey experiment
Authors: Torrente Fernando , Low Daniel , Yoris Adrian
Published: August 2022

Prior work has shown that accurately perceiving the risk for COVID-19 is associated with higher adherence to protective health behaviors, like face mask use, and more acceptance of governmental restrictive measures such as partial or complete banning of indoor activities and social gatherings. In this study we explored these associations at the beginning of the second wave of COVID-19 in Argentina through a national representative probabilistic survey that evaluated personal and contextual risk perception, self-reported compliance with protective health behaviors, attitude to governmental restrictive measures, and political orientation and psychological distress as potential modulators. Also, going beyond measures of association, here we sought to test whether messages highlighting potential risks increased acceptance of restrictive measures. Three types of messages were randomized to the participants. Two messages conveyed risk-related content (either through emotional arousal or cognitive appraisal) and the third a prosocial, altruistic content. Between March 29th and 30th, 2021, 2,894 participants were recruited (57.57% female). 74.64% of those surveyed evaluated the current health situation as “quite serious” or “very serious” and 62.03% estimated that the situation will be “worse” or “much worse” in the following 3 months. The perception of personal risk and the level of adherence to protective behaviors gradually increased with age. Through a regression model, age, perceived personal risk, and contextual risk appraisal were the variables most significantly associated with protective behaviors. In the case of the acceptance of restrictive measures, political orientation was the most associated variable. We then found messages aimed at increasing risk perception (both emotionally or cognitively focused) had a significantly greater effect on increasing the acceptance of restrictive measures than the prosocial message, mainly for government supporters but also for non-supporters. However, the level of response was also modulated by the political orientation of the participants. We propose a mechanism of “ideological anchoring” to explain that participants were responsive to risk modulation, but within the limits established by their pre-existent political views. We conclude that messages highlighting risk can help reinforce the acceptance of restrictive measures even in the presence of polarized views, but must be calibrated by age and political orientation.
Measuring catatonia motor behavior with objective instrumentation
Authors: von Känel Sofie , Nadesalingam Niluja , Alexaki Danai , Baumann Gama Daniel , Kyrou Alexandra , Lefebvre Stéphanie , Walther Sebastian
Published: August 2022

Objective

Catatonia is a neuropsychiatric syndrome, with important psychomotor features, associated with schizophrenia and other psychiatric disorders. The syndrome comprises multiple symptoms including abnormal motor control, behaviors, volition, and autonomic regulation. Catatonia assessment relies on clinical rating scales and clinicians familiar with the catatonia exam. However, objective instrumentation may aid the detection of catatonia. We aimed to investigate the relationship between movement parameters derived from actigraphy and expert ratings of catatonia symptoms measured by the Bush Francis Catatonia Rating Scale (BFCRS) and the Northoff Catatonia scale (NCS).

Methods

Eighty-six acutely ill inpatients with schizophrenia spectrum disorders were assessed with the BFCRS, the NCS, and 24 h continuous actigraphy. Non-wear and sleep periods were removed from the actigraphy data prior to analysis. Associations between total catatonia scores, derived from both BFCRS and NCS, and actigraphy parameters as well as between single BFCRS items and actigraphy parameters were calculated using Spearman's rank correlation and non-parametric ANCOVAs (Quade's ANCOVAs), respectively.

Results

Both higher BFCRS total scores (r = 0.369, p = 0.006) and NCS total scores (r = 0.384, p = 0.004) were associated with lower activity levels (AL). Higher scores on single BFCRS items such as immobility/stupor or staring were linked to lower AL (immobility/stupor: F = 17.388, p < 0.001, η2 = 0.175; staring: F = 7.849, p = 0.001, η2 = 0.162) and lower metabolic equivalents of task (MET).

Conclusion

Specific catatonia symptoms such as immobility/stupor and staring can be measured with actigraphy. This may aid the detection, staging, and monitoring of catatonia in clinical settings.
The psychological impact of COVID-19 pandemic on healthcare workers
Authors: Tong Fei , Zhang Lemeng , Huang Liping , Yang Hongxia , Wen Minni , Jiang Ling , Zou Ran , Liu Feng , Peng Wanglian , Huang Xufen , Yang Desong , Yang Hui , Yi Lili , Liu Xiaohong
Published: August 2022

Background

As unprecedented and prolonged crisis, healthcare workers (HCWs) are at high risk of developing psychological disorders. We investigated the psychological impact of COVID-19 pandemic on HCWs.

Methods

This cross-sectional study randomly recruited 439 HCWs in Hunan Cancer Hospital via a web-based sampling method from June 1st 2021 to March 31st 2022. Anxiety and depression levels were measured using Hospital Anxiety and Depression Scale (HADS). The Post Traumatic Stress Disorder (PTSD) Checklist for DSM-5 (PCL-5) was used to assess the presence and severity of PTSD. Fear was measured by modified scale of SARS. Data were collected based on these questionnaires. Differences in fear, anxiety, depression and PTSD among HCWs with different clinical characteristics were analyzed using a multivariate analysis of variance. The Cronbach's alpha scores in our samples were calculated to evaluate the internal consistency of HADS, fear scale and PCL-5.

Results

The prevalence of anxiety, depression, and PTSD in HCWs was 15.7, 9.6, and 12.8%, respectively. Females and nurses were with higher fear level (P < 0.05) and higher PTSD levels (P < 0.05). Further analysis of female HCWs revealed that PTSD levels in the 35–59 years-old age group were higher than that in other groups; while married female HCWs were with increased fear than single HCWs. The internal consistency was good, with Cronbach's α = 0.88, 0.80 and 0.84 for HADS, fear scale, and PCL, respectively.

Conclusion

Gender, marital status, and age are related to different level of psychological disorders in HCWs. Clinical supportive care should be implemented for specific group of HCWs.
An approach to involve municipal leaders into strategic decision-making for sustainability—A case study
Authors: Wälitalo Lisa , Robèrt Karl-Henrik , Broman Göran
Published: August 2022

In a previous study we tested a model for implementation of methodological support for cross-sectoral collaboration for strategic transition toward sustainability. To make the model viable long-term, practitioners emphasized the importance to recruit and engage leaders into the process upfront, however, this was also the key missing element according to the ten municipalities and regions in the action research project. Nevertheless, if addressed sufficiently, active leadership could favor other needed support, such as capacity building and merging with ongoing work. Therefore, this study aimed to design, test and evaluate an approach to better involve leaders into strategic decision-making for sustainability early on in the collaboration processes. The approach evolved as an in-depth study in one of the municipalities. A pre-assessment based on semi-structured interviews and desktop review was compiled and presented back to municipal top management during a session that included a focus group discussion to capture the leaders' feedback on the assessment as well as advice on how to make the implementation model viable long-term. Results gave that the leaders reached a good understanding of the implementation model and how current practice in the municipality related or could be related to it. As an indicator of spurred engagement and hence, a successful result, one outcome from the session was a strategic decision to carry out a thorough sustainability analysis according to the methodology that the model is supposed to implement. This work is ongoing. In addition, support for alignment with existing management systems was asked for. An evaluation of the approach itself was positive, however, pointed at the extensive work needed for the assessment. Alternative ways, such as self-assessment or peer-assessment was discussed. Forthcoming research will test and further refine the applied approach of this study to enhance strategic decision-making for sustainability while also considering the role of academia in municipal practices for sustainability.
Plastic pollution on Durance riverbank: First quantification and possible environmental measures to reduce it
Authors: Ballerini Tosca , Chaudon Nathalie , Fournier Marc , Coulomb Jean-Paul , Dumontet Bruno , Matuszak Eléonore , Poncet Justine
Published: August 2022

Plastic pollution is one of the most pressing issues of our time, with negative impacts on natural ecosystems, human health, and the climate system. The identification of top litter items discarded in the environment is essential to prioritize environmental policies to prevent plastic leakage and promote a circular economy. Here, we present the first quantification of macrolitter on three sites along Durance riverbank and one site on Lake Serre-Ponçon's beach, in the Région SUD–Provence-Alpes-Côte d'Azur, southeastern France. Data were collected through citizen science between 2019 and 2020 in three sampling occasions (autumn, winter, spring) on Durance riverbank and in 22 occasions on Lake Serre-Ponçon. A total of 25'423 litter items were categorized, of which 82% were plastics. Single-use plastic items correspond to 8.13% of total, while single-use plastic bottles are among the top 10 litter items at each site. Median litter abundance across all samples is 2,081 items/100 m survey, two orders of magnitude higher than European precautionary threshold value for marine litter (20 items/100 m survey). The majority of items (74.83%) were small and non-identifiable. Pieces of polystyrene, soft plastics and rigid plastics represented the majority of litter items in total (56.63%) and at S1 (89.28%), S2 (58.95%) and S3 (79.60%). Glass pieces corresponded to 15.83% of total litter items. Soft plastic pieces are the most abundant litter category overall and correspond to 58.85% of litter items at sampling site along Durance riverbank located in an agricultural zone, suggesting their source from agricultural plastic mulch films. Among the identifiable items, the most abundant were plastic biomedia used in waste water treatment plants and single-use beverage bottles in plastic and in glass. The development of extended producer responsibility schemes for plastic mulch films and plastic biomedia and of deposit return schemes for single-use beverage bottles is suggested as a way to prevent leakage in the environment. This work confirms the opportunity to use citizen science to gather relevant data on macrolitter items and to monitor the effectiveness of environmental regulations to reduce plastic pollution.
Acoustics Of Classrooms
Authors: Dance Stephen , Ballestero Eric
Published: August 2022

In this article we will study sound—specifically, how sound allows us to communicate in a classroom and how we can improve that communication. You will learn how sound is measured and how people called acoustics engineers help architects and designers to make sure classrooms are not too noisy—or too quiet. We even have some experiments that you can do to measure sounds at home, at school, or in a football stadium.
A Coupled Variational System for Image Decomposition along with Edges Detection
Authors: Xu Jianlou , Guo Yuying , Hao Yan , Huo Leigang
Published: August 2022

In order to better decompose the images and protect their edges, in this paper, we proposed a coupled variational system consisting of two steps. The first step, an improved weighted variational model is introduced to obtain the cartoon and texture. Using the obtained cartoon image, in the second step, a new vector function is obtained for describing the pseudo edge of the considered image by one Tikhonov regularization variational model. Because Tikhonov regularization model is equivalent to carrying out a Gaussian linear filtering, the obtained vector function is smoother. To solve the coupled system, we give the alternating direction method, primal-dual method and Gauss-Seidel iteration. Using the coupled system, we can not only separate out the cartoon and texture parts, but also extract the edge. Extensive numerical experiments are given to show the effectiveness of the proposed method compared with other variational methods.
Biased-Randomized Discrete-Event Heuristics for Dynamic Optimization with Time Dependencies and Synchronization
Authors: Castaneda Juliana , Neroni Mattia , Ammouriova Majsa , Panadero Javier , Juan Angel A.
Published: August 2022

Many real-life combinatorial optimization problems are subject to a high degree of dynamism, while, simultaneously, a certain level of synchronization among agents and events is required. Thus, for instance, in ride-sharing operations, the arrival of vehicles at pick-up points needs to be synchronized with the times at which users reach these locations so that waiting times do not represent an issue. Likewise, in warehouse logistics, the availability of automated guided vehicles at an entry point needs to be synchronized with the arrival of new items to be stored. In many cases, as operational decisions are made, a series of interdependent events are scheduled for the future, thus making the synchronization task one that traditional optimization methods cannot handle easily. On the contrary, discrete-event simulation allows for processing a complex list of scheduled events in a natural way, although the optimization component is missing here. This paper discusses a hybrid approach in which a heuristic is driven by a list of discrete events and then extended into a biased-randomized algorithm. As the paper discusses in detail, the proposed hybrid approach allows us to efficiently tackle optimization problems with complex synchronization issues.
Structural Responses of a Conceptual Microsatellite Structure Incorporating Perforation Patterns to Dynamic Launch Loads
Authors: Dawood Sarmad Dawood Salman , Harmin Mohammad Yazdi
Published: August 2022

Satellite systems undergo several operational phases during their service life, including the assembly phase, ground transportation phase, the launch phase, and the in-orbit operation phase. Among these phases, the one that imposes the highest level of loadings on the satellite is the launch phase. This phase involves a number of highly dynamic loads, all being imposed upon the satellite simultaneously. Investigation of the responses of the structural subsystem of a satellite to these loadings, namely its maximum deformations and maximum von Mises stresses, is critical if a reasonably high level of confidence is to be achieved. This confidence is in terms of ensuring that no material yielding develops in the structure as a result of the imposed launch loadings. In an earlier work, the structural subsystem of a conceptual microsatellite was designed, employing aluminum 6061 alloy as its material. It was then modified through introducing sets of parametrically defined geometric patterns as perforation patterns to remove material, towards reducing the structure’s total mass, as an alternative to employing composite materials. That effort led to a mass reduction percentage of 23.15%. The current work’s research effort focused on computing the responses of the perforated structure to three of the dynamic launch loads that are imposed upon satellites while being launched, namely quasi-static, random, and shock loads. These responses were then compared to those of the baseline, unperforated, version of the same structure. The values of these loads were taken from the relevant sources, with the values being nominal, and represented the loads that any satellite must qualify for before it can be accepted by the provider for inclusion in a launcher. After imposing these load values upon the structural design it was found that the structural responses indicated that the structure would successfully survive these loads without developing stresses that would lead to material yielding failure. This was deduced from computing the yield margins of safety for each loading case, and all margin values were positive, indicating that the structure, at its current development stage, did have sufficient capacity to withstand these loads without material yielding. This reinforced the conclusion of the earlier work, namely that the perforation concept did have sufficient merit to be further developed towards being implemented in future satellite designs.
Different Diets Change Milk Extracellular Vesicle-Protein Profile in Lactating Cows
Authors: Quan Suyu , Du Chunmei , Wang Kun , Nan Xuemei , Xiong Benhai
Published: August 2022

We previously demonstrated that the partial replacement of forage with non-forage fiber sources (NFFS) in dairy cow diets could decrease the ruminal ratio of acetate to propionate, leading to changes in circulatory and milk extracellular vesicle (EV)-miRNAs expression. This study further explored the effects of the NFFS diets on milk EV proteins, which were proposed as food bio-active ingredients in recent research. We replaced 8.97% alfalfa hay and 2.51% corn silage with 5.72% whole cotton seed and 4.73% soybean hull in the cow diet, reducing the forage neutral detergent fiber from 20.92% to 15.67%. In total 488 proteins were identified by proteome, and 65 proteins were differentially expressed in response to the NFFS diets, the functions of which were mainly enriched in immune-related pathways, including complement and coagulation cascades, phagosome, Staphylococcus aureus infection, and chemokine signaling pathway. Moreover, 57 milk EV-proteins, mainly attributed to enzymes, cytoskeletal proteins, and transport proteins, were in the top 100 most identified EV-proteins in different species and body fluids, which might be related to the biogenesis, structure, and traffic of all vesicles. The results showed that NFFS diets could influence cow milk EV-protein composition, implying that we could take effective nutritional strategies to promote the synthesis of milk functional ingredients. Combined with all our studies, NFFS diets were recommended to improve the rumen fermentation model and enrich the milk EV proteins of dairy cows.
Assessment and Effect of Mining Subsidence on Farmland in Coal–Crop Overlapped Areas: A Case of Shandong Province, China
Authors: Chen Yang , Hu Zhenqi , Li Pengyu , Li Gensheng , Yuan Dongzhu , Guo Jiaxin
Published: August 2022

Farmland protection and food security is highly focused on in China. However, coal mining has caused negative consequences to cropland in coal–crop overlapped areas (COA), especially in eastern China. Thus, revealing the spatiotemporal impact of coal mining on farmland on a large scale is crucial for coordinating coal mining and grainland protection. In this study, Shandong Province, a representative coal–grain composite area, was selected as a research case to evaluate the damage of mining subsidence on farmland. Firstly, the field investigation and mining subsidence prediction revealed the current situation and trend of farmland damage caused by mining in 2021 and 2030. Then, we evaluated the impact of cropland damage on grain yield. Finally, farmland landscape patterns, ecological environment, and social stability due to mining subsidence were discussed. The results show that: (1) the damaged cropland in 2021 was 6.40 × 104 ha, of which 1.40 × 104 ha was non-yield. By 2030, the accumulative area of damaged cropland is estimated to reach 7.52 × 104 ha. (2) By 2025 and 2030, the farmland yield will be reduced by 16.44 × 104 t and 18.45 × 104 t in overlapped areas of Shandong. (3) The subsidence led to cropland fragmentation, and the terrestrial ecosystem became an aquatic ecosystem, further intensifying the contradiction between more people and less land. This study provides a reference for coordinating coal and grain production and formulating cropland protection strategies in similar regions. Meanwhile, it also provides a scientific basis for the government to formulate land reclamation indicators, technology, management, and acceptance standards and establish and implement the reclamation reward and punishment system.
Trehalose Attenuates Oxidative Stress and Endoplasmic Reticulum Stress-Mediated Apoptosis in IPEC-J2 Cells Subjected to Heat Stress
Authors: Mo Fan , Zhou Xu , Yang Mengting , Chen Leyi , Tang Zhining , Wang Chong , Cui Yanjun
Published: August 2022

This study was carried out to investigate the effects of trehalose (Tre) on antioxidant capacity, endoplasmic reticulum stress (ERS) response and apoptosis of heat-stressed intestinal porcine epithelial cells (IPEC-J2). IPEC-J2 cells were cultured at 37 °C until the end of the experiment (control, CON); exposed to heat stress for 2 h (43 °C, HS); or pretreated with 0.1, 1, 5, 10, and 15 mM trehalose at 37 °C for 4 h prior to heat stress exposure for 2 h. The optimum level of trehalose for protecting against HS-induced cell injuries was determined to be 10 mM, as evidenced by the highest cellular viability and lowest malondialdehyde (MDA) content and lactate dehydrogenase (LDH) activity. Based on these, IPEC-J2 cells were divided into three groups: the first group was cultured at 37 °C until the end of the experiment (control, CON); the second group was exposed to heat stress for 2 h (43 °C, HS); the third group was pretreated with 10 mM trehalose for 4 h at 37 °C prior to heat stress exposure for 2 h (Tre + HS). The reactive oxygen species (ROS) content, superoxide dismutase (SOD) activity, mitochondrial membrane potential (MMP) changes, and expressions of the manganese superoxide dismutase (SOD2), ERS and apoptosis-related proteins were determined. Compared to the CON group, HS significantly increased ROS generation (p < 0.01), decreased SOD activity (p < 0.05), and downregulated protein expression of SOD2 (p < 0.01). Compared to the HS group, Tre supplementation reduced ROS levels and increased SOD activity and SOD2 expression to the levels that were comparable to the control (p < 0.05). The HS-induced ERS response was evidenced by the increased protein expressions of glucose-regulated protein 78 (GRP78) (p < 0.01), eukaryotic translation initiation factor 2α (p-eif2α) (p < 0.01), transcription activator 4 (ATF4) (p < 0.01), and the protein expression of C/EBP homologous protein (CHOP) (p < 0.01), which were the four hallmarks of ERS. The Tre + HS group showed lower expressions of GRP78 (p < 0.01), p-eif2α (p < 0.01), ATF4 (p < 0.01), and CHOP (p < 0.01) than that of the HS group. Tre pretreatment attenuated HS-induced mitochondrial apoptosis in IPEC-J2 cells, demonstrated by the increased MMP and decreased proapoptotic proteins active caspase 3, Bax, and cytochrome c (Cyt c). Taken together, trehalose can protect against HS-induced oxidative damage and endoplasmic reticulum stress-mediated apoptosis in IPEC-J2 cells. These data may provide a nutritional strategy for alleviating heat stress in pig production.
Phytase Supplementation of Four Non-Conventional Ingredients Instead of Corn Enhances Phosphorus Utilization in Yellow-Feathered Broilers
Authors: Fang Chengkun , Yu Qifang , He Jianhua , Fang Rejun , Wu Shusong
Published: August 2022

The present study was conducted to evaluate the effects of unconventional feedstuff such as wheat, broken rice, distillers dried grains with soluble (DDGS), and wheat bran, replacing 15% of the corn in the basal diet and the supplementation of bacterial phytase on nutrition digestibility. A total of 500 yellow-feathered broilers with similar body weights of 1.65 ± 0.15 kg were divided into 10 dietary treatments with 5 replicates per treatment (5 male and 5 females per cage). The AME and AIDE were significantly higher when supplied with phytase (p < 0.01) in the DDGS group. The ileal and total tract digestibility of calcium and phosphorus were significantly increased in the phytase-supplied group (p < 0.001). Additionally, the ileal digestibility of CP was increased when phytase was supplemented (p < 0.001). The results infer that the wheat, broken rice, DDGS, and wheat bran had no negative effect when replacing 15% corn. Supplementing 0.02% phytase in their diets can effectively optimize nutrient digestibility in yellow broilers.
Soft Tissue Movement in Orthognathic Surgery: Does Pre-Operative Soft Tissue Thickness Affect Movement Change?
Authors: Joachim Michael V. , Brosh Yair , Daoud Fadi , Abdelraziq Murad , Abu El-Naaj Imad , Laviv Amir
Published: August 2022

Background: The aim of this study was to examine whether the thickness of the preoperative soft tissue affects the degree of soft tissue movement relative to the hard tissue movement after orthognathic surgery. Methods: This is a retrospective examination of lateral cephalometric X-rays of patients who underwent orthognathic surgery in single medical center, during a 5-year period. Demographic and operative data were collected. Soft tissue thickness was measured in preoperative cephalometric X-ray, followed by superimposition of postoperative (>6 months) cephalometric X-rays by overlapping fixed bony points. Results: In the upper jaw, there was a decrease in the relative movement of soft tissue with an increase in the initial thickness in surgeries performing advancements up to 5 mm (r = −0.288). In mandibular advancements, there was a clear decrease in soft tissue movement ratio with an increase in the initial soft tissue thickness (r = −0.418), while there was no correlation in mandibular setback cases (r = 0.062). The same correlation between the decrease in relative soft tissue movement and the increase in initial soft tissue thickness was found in advancement genioplasty (r = −0.411). Conclusion: This research shows a general pattern of decrease in relative movement of soft tissue in orthognathic surgery correlated to an increase in its initial thickness.
BDS/IMU Integrated Auto-Navigation System of Orchard Spraying Robot
Authors: Zhang Liang , Zhu Xinghui , Huang Jingjing , Huang Jinqi , Xie Jingxin , Xiao Xu , Yin Gang , Wang Xiayu , Li Ming , Fang Kui
Published: August 2022

To improve the accuracy and reliability of orchard spraying robots, an integrated navigation system was developed, consisting of a real-time kinematic positioning-Beidou satellite navigation system (RTK-BDS) receiver, an inertial measurement unit (IMU), a navigation controller, and servo motors. Using the loose coupling combination method, an error Kalman filter algorithm based on the measurement of position and heading angle is implemented to correct the error of the inertial measurement unit in real time. Combining the kinematics model and the pure pursuit model of the spraying robot, a path-tracking control algorithm is proposed. Path planning was conducted according to the terrain characteristics of orchards. Field experiments were carried out on a spraying robot to evaluate the proposed auto-navigation system. The results showed that when the spraying robot was static, the positioning performances of BDS alone and that of the BDS/IMU combined system were similar, the positioning error was less than 1.5 cm, and the heading angle errors were within 0.3°; when the spraying robot moving alone to a straight line at the speed of 0.4 m/s, the position error of the navigation system only using BDS was less than 5.29 cm, the heading angle error was within 3°, while the position error of BDS/IMU integrated navigation system was less than 2.49 cm, and the heading angle error was within 2°. The accuracy of BDS/IMU integrated navigation system is significantly improved. When the orchard spraying robot was moving at the speed of 0.4 m/s, the maximum offset error was lower than 10.77 cm, the average offset error was not higher than 3.55 cm, and the root mean square error (RMSE) of the lateral deviation was 1.19 cm. The results showed that the proposed auto-navigation system could make the spraying robot track the pre-set path smoothly and stably.
Recent Advances in the Growth and Characterizations of SILAR-Deposited Thin Films
Authors: Soonmin Ho
Published: August 2022

Many researchers have reported on the preparation and characterization of thin films. The prepared thin films could be used in lasers, cathodic ray tubes, solar cells, infrared windows, ultraviolet light emitting diodes, sensors, supercapacitors, biologic applications, and optoelectronic applications. The properties of these thin films strongly depend on the deposition techniques. Throughout the years, many investigations into the production of various types of thin films (by using the successive ionic layer adsorption and reaction (SILAR) method) were conducted. This method attracts interest as it possesses many advantages when compared to other deposition methods. For example, large area depositions could be carried out in any substrates at lower temperatures via inexpensive instruments; moreover, a vacuum chamber is not required, it has an excellent growth rate, and the unique film properties could be controlled. In this work, metal sulfide, metal selenide, metal oxide, and metal telluride were deposited on substrates by using the SILAR method. According to the findings, both thick and thin films could be synthesized under specific conditions during the experiment. Additionally, the results showed that the number of deposition cycles, rinsing times, immersion times, and concentrations of the precursors affected the crystallinities, grain sizes, film thicknesses, surface roughness, and shapes of the obtained films. These films could be used in solar cell applications with high power conversion efficiency due to the appropriate band gap value and high absorption coefficient value.
Free-Energy Landscape Analysis of Protein-Ligand Binding: The Case of Human Glutathione Transferase A1
Authors: Nicolaï Adrien , Petiot Nicolas , Grassein Paul , Delarue Patrice , Neiers Fabrice , Senet Patrick
Published: August 2022

Glutathione transferases (GSTs) are a superfamily of enzymes which have in common the ability to catalyze the nucleophilic addition of the thiol group of reduced glutathione (GSH) onto electrophilic and hydrophobic substrates. This conjugation reaction, which occurs spontaneously but is dramatically accelerated by the enzyme, protects cells against damages caused by harmful molecules. With some exceptions, GSTs are catalytically active as homodimers, with monomers generally constituted of 200 to 250 residues organized into two subdomains. The first is the N-terminal subdomain, which contains an active site named G site, where GSH is hosted in catalytic conformation and which is generally highly conserved among GSTs. The second subdomain, hydrophobic, which binds the substrate counterpart (H site), can vary from one GST to another, resulting in structures able to recognize different substrates. In the present work, we performed all-atom molecular dynamics simulations in explicit solvent of human GSTA1 in its APO form, bound to GSH ligand and bound to GS-conjugated ligand. From MD, two probes were analyzed to (i) decipher the local conformational changes induced by the presence of the ligand and (ii) map the communication pathways involved in the ligand-binding process. These two local probes are, first, coarse-grained angles (θ,γ), representing the local conformation of the protein main chain and, second, dihedral angles χ representing the local conformation of the amino-acid side chains. From the local probes time series, effective free-energy landscapes along the amino-acid sequence were analyzed and compared between the three different forms of GSTA1. This methodology allowed us to extract a network of 33 key residues, some of them being located in the experimentally well-known binding sites G and H of GSTA1 and others being located as far as 30Å from the original binding sites. Finally, the collective motions associated with the network of key residues were established, showing a strong dynamical coupling between residues Gly14-Arg15 and Gln54-Val55, both in the same binding site (intrasite) but also between binding sites of each monomer (intersites).
Optimization of Common Iliac Artery Sonography Images via an Indigenous Water Phantom and Taguchi’s Analysis: A Feasibility Study
Authors: Wu Keng-Yi , Liang Chun-Chieh , Chuang Chao-Hsun , Pan Lung-Fa , Pan Lung-Kwang
Published: August 2022

Object: Optimization of common iliac artery sonography images using an indigenous water phantom and Taguchi’s analysis was successfully performed to improve the diagnostic accuracy in routine cardiac examination. Methods: A water phantom with two major compartments was developed, which satisfied Taguchi’s unique criterion of optimization analysis. Two or three levels were assigned to five factors, namely, (A) the probe angle, (B) water depth, (C) sonography preset frame rate, (D) amplitude gain, and (E) imaging compression ratio. The resulting Taguchi’s L18 orthogonal array contained 18 combinations of 5 factors, ensuring the same confidence level as a realm of 162 (21 × 34) combinations. The signal-to-noise ratio (S/N) was defined as the minimal difference between the practical survey and predicted areas of 50 mm2 for the sonography imaging scans. The artifact was customized by creating stenosis with a diameter of 8 mm inside a silicon pipe with a diameter of 19 mm. Results: The derived optimal parameters included (A) a zero probe angle, (B) water depth of 6 cm, (C) frame rate of 45 Hz, (D) amplitude gain of 50%, and (E) compress ratio of 50% from 3 independent measurements in each group. Further ANOVA confirmed that the frame rate was a dominant factor, with ss (sum of squared variances) of 56.6%, whereas the error and other terms were suppressed to 20.3% and 11.9%, respectively. The risks of the inappropriate setting of S/N were also discussed to avoid any misinterpretations. Conclusions: The quantified water phantom combined with Taguchi’s approach proved to be instrumental in optimizing the sonography image scan quality in routine cardiac examination.
Exploring the Potential of Moringa Leaf Extract for Mitigation of Cadmium Stress in Triticum aestivum L.
Authors: Mahmood Saba , Ahmad Waqar , Ali Zeba , Eed Emad M. , Khalifa Amany S. , Naeem Muhammad , Bibi Amir , Tahir Ayesha , Waqas Kashif , Wahid Abdul
Published: August 2022

Cadmium (Cd) is one of the most toxic metals accumulated in wheat grains. Daily intake of Cd through food is posing serious health problems. There is an urgent need to reduce the uptake and accumulation of Cd in wheat and other cereal crops. In this study, we investigated the potential of moringa leaf extract (MLE) in decreasing the Cd toxic effects in wheat (Triticum aestivum L.) cv. A.S. 2002. Sowing was carried out in pots under shade and natural light conditions. Two Cd concentrations (500 µM and 1000 µM) were applied with and without MLE (0 and 3%). Results revealed that plant growth parameters viz diameter of stem, number of leaves, leaf area, photosynthetic pigments were improved with MLE application under Cd stress. Moreover, biochemical attributes and osmolytes such as total soluble sugars, and soluble proteins, phenolic content and flavonoids were negatively effected by Cd stress and were improved under MLE treatment. Moreover, Cd stress enhanced phytotoxicity as higher ROS accumulation is observed under both stress conditions, whereas after MLE application MDA and H2O2 accumulation was significantly decreased. Based on current observations, MLE was effective in mitigating the biological and toxic effects of Cd by promoting the growth attributes and mineral contents in wheat. Further experiments are needed for discovering the molecular mechanisms underlying MLE and host plant interactions involved in Cd stress mitigation.
Demarcation of Coordinated Prevention and Control Regions in the Yangtze River Delta Based on Spatio-Temporal Variations in PM2.5 and O3 Concentrations
Authors: Wang Leilei , Zhang Zhen , Gu Zhengnan
Published: August 2022

The PM2.5 and O3 pollution situation in the Yangtze River Delta (YRD) region is increasingly complex. Existing coordinated prevention and control programs are demarcated according to administrative regions, making fine-scale pollution control difficult. This study proposed a method for delineating regional control and pollution types based on the analysis of PM2.5 and O3 pollution characteristics. Four key indicators were used to comprehensively evaluate regional pollution trends: PM2.5 concentration ρ(PM2.5), O3 concentration ρ(O3), and the rates of change r(PM2.5) and r(O3). Our results demonstrate that from 2015 to 2020, cities in the YRD show a transition in the main pollution type from PM2.5 to O3. By 2020, the main types of pollution in the YRD region are co-pollution (ρ(PM2.5) > 35 µg/m3 and ρ(O3) > 100 µg/m3), O3 pollution (ρ(PM2.5) < 35 µg/m3 and ρ(O3) > 100 µg/m3), and low pollution (ρ(PM2.5) < 35 µg/m3 and ρ(O3) < 100 µg/m3), accounting for 41.9%, 26.3%, and 30% of the study area, respectively, which is roughly consistent with the remote sensing results (40.4%, 25.6%, and 33.7%, respectively). The proposed method can predict future trends in pollution and assist in decision-making for the coordinated prevention and control of PM2.5 and O3 pollution.
ALCAM: A Novel Surface Marker on EpCAMlow Circulating Tumor Cells
Authors: Signorelli Rossana , Giret Teresa Maidana , Umland Oliver , Hadisurya Marco , Lavania Shweta , Charles Richard John Lalith , Middleton Ashley , Boone Melinda Minucci , Ergonul Ayse Burcu , Tao Weiguo Andy , Amirian Haleh , Iliuk Anton , Khan Aliya , Diaz Robert , Cortes Daniel Bilbao , Garcia-Buitrago Monica , Charles Jacob Harrys Kishore
Published: August 2022

Background: Current strategies in circulating tumor cell (CTC) isolation in pancreatic cancer heavily rely on the EpCAM and cytokeratin cell status. EpCAM is generally not considered a good marker given its transitory change during Epithelial to Mesenchymal Transition (EMT) or reverse EMT. There is a need to identify other surface markers to capture the complete repertoire of PDAC CTCs. The primary objective of the study is to characterize alternate surface biomarkers to EpCAM on CTCs that express low or negligible levels of surface EpCAM in pancreatic cancer patients. Methods: Flow cytometry and surface mass spectrometry were used to identify proteins expressed on the surface of PDAC CTCs in culture. CTCs were grown under conditions of attachment and in co-culture with naïve neutrophils. Putative biomarkers were then validated in GEMMs and patient samples. Results: Surface proteomic profiling of CTCs identified several novel protein biomarkers. ALCAM was identified as a novel robust marker in GEMM models and in patient samples. Conclusions: We identified several novel surface biomarkers on CTCs expressed under differing conditions of culture. ALCAM was validated and identified as a novel alternate surface marker on EpCAMlow CTCs.