bluuebunny/crossref_metadata_2025 huggingface dataset:
AIE-Active Photosensitizers: Manipulation of Reactive Oxygen Species Generation and Applications in Photodynamic Therapy
Authors: Yu Hao , Chen Binjie , Huang Huiming , He Zhentao , Sun Jiangman , Wang Guan , Gu Xinggui , Tang Ben Zhong
Published: May 2022

Photodynamic therapy (PDT) is a non-invasive approach for tumor elimination that is attracting more and more attention due to the advantages of minimal side effects and high precision. In typical PDT, reactive oxygen species (ROS) generated from photosensitizers play the pivotal role, determining the efficiency of PDT. However, applications of traditional PDT were usually limited by the aggregation-caused quenching (ACQ) effect of the photosensitizers employed. Fortunately, photosensitizers with aggregation-induced emission (AIE-active photosensitizers) have been developed with biocompatibility, effective ROS generation, and superior absorption, bringing about great interest for applications in oncotherapy. In this review, we review the development of AIE-active photosensitizers and describe molecule and aggregation strategies for manipulating photosensitization. For the molecule strategy, we describe the approaches utilized for tuning ROS generation by attaching heavy atoms, constructing a donor-acceptor effect, introducing ionization, and modifying with activatable moieties. The aggregation strategy to boost ROS generation is reviewed for the first time, including consideration of the aggregation of photosensitizers, polymerization, and aggregation microenvironment manipulation. Moreover, based on AIE-active photosensitizers, the cutting-edge applications of PDT with NIR irradiated therapy, activatable therapy, hypoxic therapy, and synergistic treatment are also outlined.
Effects of a Hybrid Program of Active Breaks and Responsibility on the Behaviour of Primary Students: A Mixed Methods Study
Authors: Jiménez-Parra José Francisco , Manzano-Sánchez David , Camerino Oleguer , Prat Queralt , Valero-Valenzuela Alfonso
Published: May 2022

Schools are ideal environments to promote healthy lifestyles and teach values among students. In this sense, the present study aims to verify the result of an Active Break program (AB) within the Teaching Personal and Social Responsibility (TPSR) Model in the school environment. The sample consisted of two teachers/tutors from the sixth year of Primary Education and 51 pupils, aged between 11 and 13 years, who were divided into an experimental group (n = 26) and a control group (n = 25). The intervention program lasted 3 months, in which the hybridised methodology was applied during 100% of the weekly classes, computing a total of 156 sessions by the end of the study. It was a quasi-experimental study design that used a mixed methodology combining a systematic observational analysis with semi-structured interviews. The results showed an evolution in the behaviour of the teacher from the experimental group from a controlling style to one centred on the transfer of autonomy, while the teacher from the control group primarily used strategies based on the imposition of tasks and the establishment of organisation, which caused an increase in disruptive behaviours among students. We conclude that the program is adaptable to Primary Education and can be extended to any educational environment to improve the classroom climate and attract the attention of students and, finally, allows for the promotion of new teaching strategies.
Nanoparticles as Physically- and Biochemically-Tuned Drug Formulations for Cancers Therapy
Authors: Foglizzo Valentina , Marchiò Serena
Published: May 2022

Malignant tumors originate from a combination of genetic alterations, which induce activation of oncogenes and inactivation of oncosuppressor genes, ultimately resulting in uncontrolled growth and neoplastic transformation. Chemotherapy prevents the abnormal proliferation of cancer cells, but it also affects the entire cellular network in the human body with heavy side effects. For this reason, the ultimate aim of cancer therapy remains to selectively kill cancer cells while sparing their normal counterparts. Nanoparticle formulations have the potential to achieve this aim by providing optimized drug delivery to a pathological site with minimal accumulation in healthy tissues. In this review, we will first describe the characteristics of recently developed nanoparticles and how their physical properties and targeting functionalization are exploited depending on their therapeutic payload, route of delivery, and tumor type. Second, we will analyze how nanoparticles can overcome multidrug resistance based on their ability to combine different therapies and targeting moieties within a single formulation. Finally, we will discuss how the implementation of these strategies has led to the generation of nanoparticle-based cancer vaccines as cutting-edge instruments for cancer immunotherapy.
FLT3-ITD in Children with Early T-cell Precursor (ETP) Acute Lymphoblastic Leukemia: Incidence and Potential Target for Monitoring Minimal Residual Disease (MRD)
Authors: Lo Nigro Luca , Andriano Nellina , Buldini Barbara , Silvestri Daniela , Villa Tiziana , Locatelli Franco , Parasole Rosanna , Barisone Elena , Testi Anna Maria , Biondi Andrea , Valsecchi Maria Grazia , Rizzari Carmelo , Conter Valentino , Basso Giuseppe , Cazzaniga Giovanni
Published: May 2022

Early T-cell precursor (ETP) is an aggressive form of acute lymphoblastic leukemia (ALL), associated with high risk of relapse. This leukemia subtype shows a higher prevalence of mutations, typically associated with acute myeloid leukemia (AML), including RAS and FLT3 mutations. FLT3-ITD was identified in 35% cases of adult ETP-ALL, but data in the pediatric counterpart are lacking. ETPs frequently lack immunoglobulin (IG) and T-cell receptor (TR) gene rearrangements, used for minimal residual disease (MRD) monitoring. Among 718 T-ALL enrolled in Italy into AIEOP-BFM-ALL2000, AIEOP-ALLR2006, and AIEOP-BFM-ALL2009 consecutive protocols, 86 patients (12%) were identified as ETP and 77 out of 86 children were studied for the presence of FLT3-ITD. A total of 10 out of 77 (13%) ETP cases were FLT3-ITD positive. IG/TR MRD monitoring was feasible only in four cases. FLT3-ITD MRD monitoring was performed using real-time PCR in all FLT3-ITD positive ETP cases. A comparison between IG/TR and FLT3-ITD resulted in comparable findings. Our study demonstrated that the FLT3-ITD prevalence in children was lower (13%) than that reported in adult ETP-ALL. FLT3-ITD can be used as a marker for sensitive molecular MRD monitoring in ETP-ALL when IG/TR markers are not available, potentially selecting those patients who should spare allogeneic hematopoietic stem cell transplantation (HSCT). Finally, the FLT3 pathway is a robust druggable target in this aggressive form of leukemia.
How Phosphofructokinase-1 Promotes PI3K and YAP/TAZ in Cancer: Therapeutic Perspectives
Authors: Simula Luca , Alifano Marco , Icard Philippe
Published: May 2022

PI3K/AKT is one of the most frequently altered signaling pathways in human cancers, supporting the activation of many proteins sustaining cell metabolism, proliferation, and aggressiveness. Another important pathway frequently altered in cancer cells is the one regulating the YAP/TAZ transcriptional coactivators, which promote the expression of genes sustaining aerobic glycolysis (such as WNT, MYC, HIF-1), EMT, and drug resistance. Of note, the PI3K/AKT pathway can also regulate the YAP/TAZ one. Unfortunately, although PI3K and YAP inhibitors are currently tested in highly resistant cancers (both solid and hematologic ones), several resistance mechanisms may arise. Resistance mechanisms to PI3K inhibitors may involve the stimulation of alternative pathways (such as RAS, HER, IGFR/AKT), the inactivation of PTEN (the physiologic inhibitor of PI3K), and the expression of anti-apoptotic Bcl-xL and MCL1 proteins. Therefore, it is important to improve current therapeutic strategies to overcome these limitations. Here, we want to highlight how the glycolytic enzyme PFK1 (and its product F-1,6-BP) promotes the activation of both PI3K/AKT and YAP/TAZ pathways by several direct and indirect mechanisms. In turn, PI3K/AKT and YAP/TAZ can promote PFK1 activity and F-1,6-BP production in a positive feedback loop, thus sustaining the Warburg effect and drug resistance. Thus, we propose that the inhibition of PFK1 (and of its key activator PFK2/PFKFB3) could potentiate the sensitivity to PI3K and YAP inhibitors currently tested. Awaiting the development of non-toxic inhibitors of these enzymes, we propose to test the administration of citrate at a high dosage, because citrate is a physiologic inhibitor of both PFK1 and PFK2/PFKFB3. Consistently, in various cultured cancer cells (including melanoma, sarcoma, hematologic, and epithelial cancer cells), this “citrate strategy” efficiently inhibits the IGFR1/AKT pathway, promotes PTEN activity, reduces Bcl-xL and MCL1 expression, and increases sensitivity to standard chemotherapy. It also inhibits the development of sarcoma, pancreatic, mammary HER+ and lung RAS-driven tumors in mice without apparent toxicities.
Non-Invasive Characterization of Experimental Bone Metastasis in Obesity Using Multiparametric MRI and PET/CT
Authors: Gregoric Gasper , Gaculenko Anastasia , Nagel Lisa , Popp Vanessa , Maschauer Simone , Prante Olaf , Saake Marc , Schett Georg , Uder Michael , Ellmann Stephan , Bozec Aline , Bäuerle Tobias
Published: May 2022

The growth of primary tumors and metastases is associated with excess body fat. In bone metastasis formation, the bone marrow microenvironment, and particularly adipocytes, play a pivotal role as growth mediators of disseminated tumor cells in the bone marrow. The aim of the present study is to non-invasively characterize the pathophysiologic processes in experimental bone metastasis resulting from accelerated tumor progression within adipocyte-rich bone marrow using multimodal imaging from magnetic resonance imaging (MRI) and positron emission tomography/computed tomography (PET/CT). To achieve this, we have employed small animal models after the administration of MDA-MB 231 breast cancer and B16F10 melanoma cells into the bone of nude rats or C57BL/6 mice, respectively. After tumor cell inoculation, ultra-high field MRI and µPET/CT were used to assess functional and metabolic parameters in the bone marrow of control animals (normal diet, ND), following a high-fat diet (HFD), and/or treated with the peroxisome proliferator-activated receptor-gamma (PPARγ) antagonist bisphenol-A-diglycidylether (BADGE), respectively. In the bone marrow of nude rats, dynamic contrast-enhanced MRI (DCE-MRI) and diffusion-weighted imaging (DWI), as well as [18F]fluorodeoxyglucose-PET/CT([18F]FDG-PET/CT), was performed 10, 20, and 30 days after tumor cell inoculation, followed by immunohistochemistry. DCE-MRI parameters associated with blood volume, such as area under the curve (AUC), were significantly increased in bone metastases in the HFD group 30 days after tumor cell inoculation as compared to controls (p < 0.05), while the DWI parameter apparent diffusion coefficient (ADC) was not significantly different between the groups. [18F]FDG-PET/CT showed an enhanced glucose metabolism due to increased standardized uptake value (SUV) at day 30 after tumor cell inoculation in animals that received HFD (p < 0.05). BADGE treatment resulted in the inversion of quantitative DCE-MRI and [18F]FDG-PET/CT data, namely a significant decrease in AUC and SUV in HFD-fed animals as compared to ND-fed controls (p < 0.05). Finally, immunohistochemistry and qPCR confirmed the HFD-induced stimulation in vascularization and glucose activity in murine bone metastases. In conclusion, multimodal and multiparametric MRI and [18F]FDG-PET/CT were able to derive quantitative parameters in bone metastases, revealing an increase in vascularization and glucose metabolism following HFD. Thus, non-invasive imaging may serve as a biomarker for assessing the pathophysiology of bone metastasis in obesity, opening novel options for therapy and treatment monitoring by MRI and [18F]FDG-PET/CT.
Cellular Origins and Pathogenesis of Gastrointestinal NK- and T-Cell Lymphoproliferative Disorders
Authors: Hue Susan Swee-Shan , Ng Siok-Bian , Wang Shi , Tan Soo-Yong
Published: May 2022

The intestinal immune system, which must ensure appropriate immune responses to both pathogens and commensal microflora, comprises innate lymphoid cells and various T-cell subsets, including intra-epithelial lymphocytes (IELs). An example of innate lymphoid cells is natural killer cells, which may be classified into tissue-resident, CD56bright NK-cells that serve a regulatory function and more mature, circulating CD56dim NK-cells with effector cytolytic properties. CD56bright NK-cells in the gastrointestinal tract give rise to indolent NK-cell enteropathy and lymphomatoid gastropathy, as well as the aggressive extranodal NK/T cell lymphoma, the latter following activation by EBV infection and neoplastic transformation. Conventional CD4+ TCRαβ+ and CD8αβ+ TCRαβ+ T-cells are located in the lamina propria and the intraepithelial compartment of intestinal mucosa as type ‘a’ IELs. They are the putative cells of origin for CD4+ and CD8+ indolent T-cell lymphoproliferative disorders of the gastrointestinal tract and intestinal T-cell lymphoma, NOS. In addition to such conventional T-cells, there are non-conventional T-cells in the intra-epithelial compartment that express CD8αα and innate lymphoid cells that lack TCRs. The central feature of type ‘b’ IELs is the expression of CD8αα homodimers, seen in monomorphic epitheliotropic intestinal T-cell lymphoma (MEITL), which primarily arises from both CD8αα+ TCRαβ+ and CD8αα+ TCRγδ+ IELs. EATL is the other epitheliotropic T-cell lymphoma in the GI tract, a subset of which arises from the expansion and reprograming of intracytoplasmic CD3+ innate lymphoid cells, driven by IL15 and mutations of the JAK-STAT pathway.
Diphenyleneiodonium Triggers Cell Death of Acute Myeloid Leukemia Cells by Blocking the Mitochondrial Respiratory Chain, and Synergizes with Cytarabine
Authors: Dakik Hassan , El Dor Maya , Bourgeais Jérôme , Kouzi Farah , Herault Olivier , Gouilleux Fabrice , Zibara Kazem , Mazurier Frédéric
Published: May 2022

Acute myeloid leukemia (AML) is characterized by the accumulation of undifferentiated blast cells in the bone marrow and blood. In most cases of AML, relapse frequently occurs due to resistance to chemotherapy. Compelling research results indicate that drug resistance in cancer cells is highly dependent on the intracellular levels of reactive oxygen species (ROS). Modulating ROS levels is therefore a valuable strategy to overcome the chemotherapy resistance of leukemic cells. In this study, we evaluated the efficiency of diphenyleneiodonium (DPI)—a well-known inhibitor of ROS production—in targeting AML cells. Results showed that although inhibiting cytoplasmic ROS production, DPI also triggered an increase in the mitochondrial ROS levels, caused by the disruption of the mitochondrial respiratory chain. We also demonstrated that DPI blocks mitochondrial oxidative phosphorylation (OxPhos) in a dose-dependent manner, and that AML cells with high OxPhos status are highly sensitive to treatment with DPI, which synergizes with the chemotherapeutic agent cytarabine (Ara-C). Thus, our results suggest that targeting mitochondrial function with DPI might be exploited to target AML cells with high OxPhos status.
Resistance to Gemcitabine in Pancreatic Ductal Adenocarcinoma: A Physiopathologic and Pharmacologic Review
Authors: Koltai Tomas , Reshkin Stephan Joel , Carvalho Tiago M. A. , Di Molfetta Daria , Greco Maria Raffaella , Alfarouk Khalid Omer , Cardone Rosa Angela
Published: May 2022

Pancreatic ductal adenocarcinoma (PDAC) is a very aggressive tumor with a poor prognosis and inadequate response to treatment. Many factors contribute to this therapeutic failure: lack of symptoms until the tumor reaches an advanced stage, leading to late diagnosis; early lymphatic and hematic spread; advanced age of patients; important development of a pro-tumoral and hyperfibrotic stroma; high genetic and metabolic heterogeneity; poor vascular supply; a highly acidic matrix; extreme hypoxia; and early development of resistance to the available therapeutic options. In most cases, the disease is silent for a long time, andwhen it does become symptomatic, it is too late for ablative surgery; this is one of the major reasons explaining the short survival associated with the disease. Even when surgery is possible, relapsesare frequent, andthe causes of this devastating picture are the low efficacy ofand early resistance to all known chemotherapeutic treatments. Thus, it is imperative to analyze the roots of this resistance in order to improve the benefits of therapy. PDAC chemoresistance is the final product of different, but to some extent, interconnected factors. Surgery, being the most adequate treatment for pancreatic cancer and the only one that in a few selected cases can achieve longer survival, is only possible in less than 20% of patients. Thus, the treatment burden relies on chemotherapy in mostcases. While the FOLFIRINOX scheme has a slightly longer overall survival, it also produces many more adverse eventsso that gemcitabine is still considered the first choice for treatment, especially in combination with other compounds/agents. This review discusses the multiple causes of gemcitabine resistance in PDAC.
Comprehensive Analysis of Acquired Genetic Variants and Their Prognostic Impact in Systemic Mastocytosis
Authors: González-López Oscar , Muñoz-González Javier I. , Orfao Alberto , Álvarez-Twose Iván , García-Montero Andrés C.
Published: May 2022

Systemic mastocytosis (SM) is a rare clonal haematopoietic stem cell disease in which activating KIT mutations (most commonly KIT D816V) are present in virtually every (>90%) adult patient at similar frequencies among non-advanced and advanced forms of SM. The KIT D816V mutation is considered the most common pathogenic driver of SM. Acquisition of this mutation early during haematopoiesis may cause multilineage involvement of haematopoiesis by KIT D816V, which has been associated with higher tumour burden and additional mutations in other genes, leading to an increased rate of transformation to advanced SM. Thus, among other mutations, alterations in around 30 genes that are also frequently mutated in other myeloid neoplasms have been reported in SM cases. From these genes, 12 (i.e., ASXL1, CBL, DNMT3A, EZH2, JAK2, KRAS, NRAS, SF3B1, RUNX1, SF3B1, SRSF2, TET2) have been recurrently reported to be mutated in SM. Because of all the above, assessment of multilineage involvement of haematopoiesis by the KIT D816V mutation, in the setting of multi-mutated haematopoiesis as revealed by a limited panel of genes (i.e., ASXL1, CBL, DNMT3A, EZH2, NRAS, RUNX1 and SRSF2) and associated with a poorer patient outcome, has become of great help to identify SM patients at higher risk of disease progression and/or poor survival who could benefit from closer follow-up and eventually also early cytoreductive treatment.
Stability of a NiAl2O4 Derived Catalyst in the Ethanol Steam Reforming in Reaction-Regeneration Cycles: Effect of Reduction Temperature
Authors: Iglesias-Vázquez Sergio , Valecillos José , Remiro Aingeru , Bilbao Javier , Gayubo Ana Guadalupe
Published: May 2022

The catalyst regeneration is still a challenge to make the ethanol steam reforming (ESR) process feasible for sustainable H2 production. NiAl2O4 spinel derived catalysts are highly active and selective for ESR, but they require avoiding irreversible deactivation to ensure their regeneration. Their stability depends on the catalyst structure, and herein we report different Ni/Al2O3-NiAl2O4 catalysts obtained upon reduction of a NiAl2O4 spinel at 700, 750, or 850 °C. The catalysts were tested in ESR reaction-regeneration cycles, with reaction at 600 °C and regeneration by coke combustion at 850 °C followed by reduction at the corresponding temperature. The fresh, spent, and regenerated catalysts were characterized using X-ray diffraction, N2 physisorption, temperature programmed reduction and oxidation, and scanning electron microscopy. The irreversible deactivation is due to Ni volatilization and catalyst particle fragmentation. These phenomena are prompted by a high filamentous carbon deposition favored by the Al2O3 content in the catalyst. The reduction in the 700–750 °C range is optimum for controlling the Al2O3 content, increasing the NiAl2O4/Al2O3 ratio in the resulting catalyst. These catalysts show a period of partial reversible deactivation by coke with a change in the H2 formation mechanism reaching a pseudo-stable state with a H2 yield of 40% and a reproducible performance in successive reaction-regeneration cycles.
Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor
Authors: Hollenbach Rebecca , Muller Delphine , Delavault André , Syldatk Christoph
Published: May 2022

Glycolipids are a class of biodegradable biosurfactants that are non-toxic and based on renewables, making them a sustainable alternative to petrochemical surfactants. Enzymatic synthesis allows a tailor-made production of these versatile compounds using sugar and fatty acid building blocks with rationalized structures for targeted applications. Therefore, glycolipids can be comprehensively designed to outcompete conventional surfactants regarding their physicochemical properties. However, enzymatic glycolipid processes are struggling with both sugars and fatty acid solubilities in reaction media. Thus, continuous flow processes represent a powerful tool in designing efficient syntheses of sugar esters. In this study, a continuous enzymatic glycolipid production catalyzed by Novozyme 435® is presented as an unprecedented concept. A biphasic aqueous–organic system was investigated, allowing for the simultaneous solubilization of sugars and fatty acids. Owing to phase separation, the remaining non-acylated glucose was easily separated from the product stream and was refed to the reactor forming a closed-loop system. Productivity in the continuous process was higher compared to a batch one, with space–time yields of up to 1228 ± 65 µmol/L/h. A temperature of 70 °C resulted in the highest glucose-6-O-decanoate concentration in the Packed Bed Reactor (PBR). Consequently, the design of a continuous biocatalytic production is a step towards a more competitive glycolipid synthesis in the aim for industrialization.
Facile Preparation of Porous Carbon Flake-Supported Nickel Nanoplates as Effective Catalysts for Methanol Electrooxidation
Authors: Aldalbahi Ali , El-Newehy Mohamed H. , El-Hamshary Hany , Samuel Edmund , Yoon Sam S.
Published: May 2022

Herein, we report a facile and efficient method for fabricating porous carbon flakes (PCFs)-supported nickel nanoplates (Ni NPs) as electrocatalysts for methanol oxidation in alkaline media. The catalyst was fabricated in one step using molten salt synthesis. Various techniques were used to characterize the morphology and composition of the Ni NPs@PCFs catalyst, and these revealed that the Ni NPs were dispersed finely across the PCFs with a highly crystalline structure. The Ni NPs@PCFs catalyst demonstrated high electrocatalytic activity for methanol oxidation (121 mA/cm2 vs. Ag/AgCl), and it had an onset potential of 0.35 V. It also exhibited high stability in an alkaline electrolyte for the duration of the experiment (up to 2000 s).
Integrated Analysis of Cancer Tissue and Vitreous Humor from Retinoblastoma Eyes Reveals Unique Tumor-Specific Metabolic and Cellular Pathways in Advanced and Non-Advanced Tumors
Authors: Babu Vishnu Suresh , Mallipatna Ashwin , SA Deepak , Dudeja Gagan , Kannan Ramaraj , Shetty Rohit , Nair Archana Padmanabhan , Gundimeda Seetharamanjaneyulu , Chaurasia Shyam S. , Verma Navin Kumar , Lakshminarayanan Rajamani , Heymans Stephane , Barathi Veluchamy A. , Guha Nilanjan , Ghosh Arkasubhra
Published: May 2022

Retinoblastoma (Rb) is a pediatric intraocular malignancy that is proposed to originate from maturing cone cell precursors in the developing retina. The molecular mechanisms underlying the biological and clinical behaviors are important to understand in order to improve the management of advanced-stage tumors. While the genetic causes of Rb are known, an integrated understanding of the gene expression and metabolic processes in tumors of human eyes is deficient. By integrating transcriptomic profiling from tumor tissues and metabolomics from tumorous eye vitreous humor samples (with healthy, age-matched pediatric retinae and vitreous samples as controls), we uncover unique functional associations between genes and metabolites. We found distinct gene expression patterns between clinically advanced and non-advanced Rb. Global metabolomic analysis of the vitreous humor of the same Rb eyes revealed distinctly altered metabolites, indicating how tumor metabolism has diverged from healthy pediatric retina. Several key enzymes that are related to cellular energy production, such as hexokinase 1, were found to be reduced in a manner corresponding to altered metabolites; notably, a reduction in pyruvate levels. Similarly, E2F2 was the most significantly elevated E2F family member in our cohort that is part of the cell cycle regulatory circuit. Ectopic expression of the wild-type RB1 gene in the Rb-null Y79 and WERI-Rb1 cells rescued hexokinase 1 expression, while E2F2 levels were repressed. In an additional set of Rb tumor samples and pediatric healthy controls, we further validated differences in the expression of HK1 and E2F2. Through an integrated omics analysis of the transcriptomics and metabolomics of Rb, we uncovered a significantly altered tumor-specific metabolic circuit that reduces its dependence on glycolytic pathways and is governed by Rb1 and HK1.
Influence of the Support Composition on the Activity of Cobalt Catalysts Supported on Hydrotalcite-Derived Mg-Al Mixed Oxides in Ammonia Synthesis
Authors: Zybert Magdalena , Ronduda Hubert , Dziewulska Aleksandra , Sobczak Kamil , Ostrowski Andrzej , Patkowski Wojciech , Raróg-Pilecka Wioletta
Published: May 2022

Recently, catalysts with hydrotalcites and hydrotalcite-derived compounds have attracted particular interest due to their specific properties, mostly well-developed texture, high thermal stability, and favorable acid–base properties. In this work, we report the investigation of ammonia synthesis on barium-promoted cobalt catalysts supported on hydrotalcite-derived Mg-Al mixed oxides with different Mg/Al molar ratios. The obtained catalysts were characterized using TGA-MS, nitrogen physisorption, XRPD, TEM, STEM-EDX, H2-TPD, CO2-TPD, and tested in ammonia synthesis (470 °C, 6.3 MPa, H2/N2 = 3). The studies revealed that the prepared Mg-Al mixed oxides are good candidates as support materials for Co-based catalysts. However, interestingly, the support composition does not influence the activity of Ba/Co/Mg-Al catalysts. The change in Mg/Al molar ratio in the range of 2–5 did not significantly change the catalyst properties. All the catalysts are characterized by similar textural, structural, and chemisorption properties. The similar density of basic sites on the surface of the studied catalysts was reflected in their comparable performance in ammonia synthesis.
Metal–Organic Cages: Applications in Organic Reactions
Authors: Chen Shangjun , Chen Li-Jun
Published: May 2022

Supramolecular metal–organic cages, a class of molecular containers formed via coordination-driven self-assembly, have attracted sustained attention for their applications in catalysis, due to their structural aesthetics and unique properties. Their inherent confined cavity is considered to be analogous to the binding pocket of enzymes, and the facile tunability of building blocks offers a diverse platform for enzyme mimics to promote organic reactions. This minireview covers the recent progress of supramolecular metal–organic coordination cages for boosting organic reactions as reaction vessels or catalysts. The developments in the utilizations of the metal–organic cages for accelerating the organic reactions, improving the selectivity of the reactions are summarized. In addition, recent developments and successes in tandem or cascade reactions promoted by supramolecular metal–organic cages are discussed.
Perceived Changes in Emotions, Worries and Everyday Behaviors in Children and Adolescents Aged 5–18 Years with Type 1 Diabetes during the COVID-19 Pandemic
Authors: Sfinari Anastasia , Pervanidou Panagiota , Chouliaras Giorgos , Zoumakis Emmanouil , Vasilakis Ioannis A. , Nicolaides Nicolas C. , Kanaka-Gantenbein Christina
Published: May 2022

The COVID-19 pandemic and the consequent restrictive measures may be related to increased stress and anxiety and to changes in daily behaviors. Children with type 1 diabetes (T1D) are a vulnerable group due to their difficulties in achieving glycemic control and to their medical and psychological comorbidities. The purpose of the current study was to the investigate the changes on emotional and behavioral parameters in children with T1D due to the Coronavirus crisis. A total of 152 children and adolescents, aged 5–18, were studied: 114 (62 boys) with T1D and 38 (19 boys) healthy volunteers (HV) (controls). The study was performed at the Diabetes Center, Aghia Sofia Children’s Hospital, during the first national lockdown in Greece. The CRISIS questionnaire was completed by parents/caregivers. The data were collected in May 2020 and referred to two time-points: three months prior (before the pandemic), and the past two weeks. During the lockdown, it was observed significant aggravation in the “Emotion/Worries (EW)” symptoms in both groups (logEW-before vs. logEW-during the crisis, T1D: 2.66 ± 0.23 vs. 3.00 ± 0.21, p < 0.001 and HV: 2.62 ± 0.16 vs. 2.83 ± 0.18, p < 0.001). Deterioration of “ΕW” was recorded for 93.0% of those with T1D and 92.1% of the HV. “EW” during the lockdown were affected by: previous psychological condition, COVID-related concerns, and “Life Changes due to the COVID-19 crisis in the past two weeks (LC)”. Deterioration was observed in the “daily behaviors” and “use of digital media” for all of the children. The crisis and the associated restrictions negatively affected both the lifestyle parameters and the behavioral and emotional variables of the children with T1D.
Novel Biomarkers of Heart Failure in Pediatrics
Authors: Senekovič Kojc Teja , Marčun Varda Nataša
Published: May 2022

Novel biomarkers of heart failure are the subject of numerous studies. Biomarkers of heart failure can be determined in the blood and in the urine. Seven groups of biomarkers of heart failure based on pathophysiological mechanisms are presented in this review, namely biomarkers of myocardial stretch, myocyte injury, myocardial remodeling, biomarkers of inflammation, renal dysfunction, neurohumoral activation, and oxidative stress. Studies of biomarkers in the pediatric population are scarce, therefore, further investigation is needed for reliable prognostic and therapeutic implications. The future of biomarker use is in multimarker panels that include a combination of biomarkers with different pathophysiological mechanisms in order to improve their diagnostic and prognostic predictive value.
Using Next-Generation Sequencing and Bioinformatic Methods to Predict New Genes That May Be Regulated by CD47 in Oral Squamous Cell Carcinoma
Authors: Tseng Chung-Chih , Tsou Chen-Han , Huang Shi-Ying , Wu Chia-Wei , Hsieh Tsung-Hua
Published: May 2022

Oral squamous cell carcinoma (OSCC) is one of the most common cancers in the world, and the incidence and death rate of OSCC in men is twice that of women. CD47 is a ubiquitous cell surface transmembrane protein, also known as integrin-related protein (IAP). Previous studies have pointed out that CD47 can inhibit the growth of OSCC, but the detailed mechanism is not clear. This study aimed to explore the effect of CD47 gene expression profiles in OSCC. The OSCC cell lines, OECM-1 and OC-2, overexpressed CD47, and the expression profiles of mRNAs were analyzed through next-generation sequencing (NGS) with a bioinformatic approach. A total of 14 differentially expressed genes (DEGs) were listed. In addition, ingenuity pathway analysis (IPA) was used to analyze the molecular function (MF), biological process (BP), and cellular component (CC) network signaling. The human protein atlas (HPA) database was used to analyze gene expression and the survivability of human cancer. The results found that HSPA5, HYOU1, and PDIA4 were involved in the IPA network and when highly expressed, mediated the survivability of cancer. In addition, HSPA5 was positively and significantly correlated with CD47 expression (p < 0.0001) and induced by CD47-overexpression in the OECM-1 and OC-2 OSCC cancer cell lines. These findings provide important insights into possible new diagnostic strategies, including unfolded protein for OSCC-targeting CD47.
Planning a Notable CCS Pilot-Scale Project: A Case Study in France, Paris Basin—Ile-de-France
Authors: Veloso Fernanda M. L. , Gravaud Isaline , Mathurin Frédéric A. , Ben Rhouma Sabrine
Published: May 2022

Few commercial-scale carbon capture and storage (CCS) projects are currently operating in the world, with almost all in the USA and China. Despite a high number of CCS pilot-scale projects achieved in Europe, only two commercial-scale projects are operating today. The goal of this study is to present a case study in France to select a promising location to deploy a notable CCS pilot-scale project based on a multicriteria regional-scale approach. The methodology applied in this case study describes and assesses different aspects involved in CCS technology at the regional scale, and then an evaluation of economic key performance indicators (KPI) of CCS is carried out. The assessment at the regional scale gives an overview of where CCS could be applied, when CCS could be deployed and how to launch CCS considering the needs and concerns of stakeholders in the region. Technical aspects were mapped, such as the location of irreducible CO2 sources and long-lasting emissions and the location of storage resources and existing potential transport infrastructures. We identified the waste-to-energy and chemical sectors as the main CO2 sources in the region. An economic analysis of a hypothetical scenario of CCS deployment was elaborated considering three of the higher emitters in the region. A CCS scenario in the Paris Basin region with a deployment between 2027 and 2050 indicates a low CO2 cost per ton avoided between 43 EUR/t and 70 EUR/t for a cumulated total of 25 Mt and 16 Mt, respectively, of CO2 captured and stored for 26 years, including 7.7 Mt of CO2 from biomass (potential negative emissions). Storage maturity and availability of the resource are the most uncertain parameters of the scenario, although they are the key elements to push investment in capture facilities and transport. Geological storage pilot projects are mandatory to prove storage resource and should be located in strategic locations close to potential CO2 sources in case of confirmation of proven resources. Well-perceived pilot-scale projects are the first step to start engaging in deciding and investing in commercial-scale CCS projects.
Unraveling the Multiple Drivers of Greening-Browning and Leaf Area Variability in a Socioeconomically Sensitive Drought-Prone Region
Authors: Bageshree K. , Abhishek  , Kinouchi Tsuyoshi
Published: May 2022

The complex attribution of climatic, hydrologic, and anthropogenic drivers to vegetation and agricultural production and their consequential societal impacts are not well understood, especially in socioeconomically sensitive states like Maharashtra, India. Here, we analyzed trends and variability in the MODIS leaf area index (LAI) time series, along with spatiotemporal patterns in precipitation, groundwater storage, agriculture statistics, and irrigation infrastructure, to identify their influences on the vegetation response and discuss their implications for farmers. The state showed greening in all biomes except forests, with a net gain of 17.478 × 103 km2 of leaf area during 2003–2019, where more than 70% of the trend in LAI is represented in croplands. Maximum greening was observed in irrigated croplands, attributable to increased crop productivity, whereas inadequate irrigation facilities with erratic rainfall patterns and droughts were primarily responsible for cropland browning. We discerned the dynamics and variability of vegetation response by incorporating a spectrum of synergistic feedbacks from multiple confounding drivers and found that uneven distribution of water availability across the administrative divisions governed the quantitative distinction in leaf area change. Despite the observed greening trends, the state witnessed a high number of farmer suicides related to droughts and agriculture failures hampering their socioeconomic security; therefore, improved irrigation infrastructure and comprehensive policy interventions are crucial for abatement of farmer distress.
Preliminary Checklist of Malaxidinae and Liparidinae Representatives (Orchidaceae, Malaxideae) from Bali and Lombok Islands (Indonesia) with New Records
Authors: Margońska Hanna B. , Champion Jeffrey , Lipińska Monika M.
Published: May 2022

Bali and Lombok are well known as popular touristic destinations. Both islands are accessible for potential floristic research, but surprisingly, information on their orchid flora is rather scarce and random. During our visits, among other orchid groups, we had an opportunity to observe several representatives of the tribe Malaxideae, which is considered to be one of the biggest and most interesting orchid groups with over 1700 taxa (including synonyms). In this paper, we present the first attempt at organizing the knowledge about these unique, often underestimated, orchids in Bali and Lombok, and we report several new records for both islands. For Bali, there are six from the genus Crepidium and four from Liparis sensu lato, and for Lombok, one from Crepidium and three from Liparis sensu lato.
On the Resistance to Buckling Loads of Idealized Hull Structures: FE Analysis on Designed-Stiffened Plates
Authors: Prabowo Aditya , Ridwan Ridwan , Muttaqie Teguh
Published: May 2022

In this paper, the buckling analysis on simply supported rectangular plates and stiffened panels is carried out. Three different plate thicknesses were proposed (i.e., 3 mm, 4 mm, and 5 mm). The thickness of the longitudinal stringers and sub-stiffeners were also varied. The material that was used was marine grade steel. The load versus the displacement curve and the total energy were measured. The buckling analysis results were examined via finite element (FE) computation. To ensure that the results of the methodology for the finite element were reliable, the benchmark buckling analysis of the experimental test was reconstructed. For the selection of mesh size, the element to thickness ratio method (ELT) was used. The results revealed that the thickness of the plate increases the strength of the stiffened panel. The plate thickness of 5 mm increased by 65.7% and 20.61% compared with the 3 mm and 4 mm plate thicknesses. A change in the thickness of the sub-stiffeners does not significantly change the strength of the stiffened panels. Material S355JR-EN10210 produced a higher ultimate panel collapse load compared with S235JR-EN10025 (A) and S235JR-EN10025 (B).
Towards a Better Interior Design for the Academic Library at College of Education—King Faisal University
Authors: Abouelela Amira
Published: May 2022

Academic libraries include college and university libraries. Interior design is an important way to bring about the required change in transforming academic libraries from a repository of books into places for research and communication. While interior design plays a major role in providing an innovative functional design in academic libraries within universities, the problem of the study lies in the importance of academic libraries and their main role in university education institutions in encouraging and supporting scientific research among students and faculty members. According to the role of interior design in designing academic libraries, the researcher finds that there are problems with interior design in the academic library at the College of Education. It needs to be re-designed to improve functionality and aesthetics and enhance the internal space for users to achieve effective use of the internal library space. The study aims to create a better design of interior environments in academic libraries in order to feature creativity and innovation. The research method is descriptive–analytical to describe and analyze the current interior design of the academic library at the College of Education at King Faisal University and collect real information about the library and the problems of interior design. Then, it was presented through the opinion poll tool to the beneficiaries to know their opinions about the current design of the library and to benefit from them in the design proposal of the academic library according to the correct design considerations. Then, the opinion poll tool was displayed again according to the design proposal to know the views of the beneficiaries again to contribute to providing an appropriate research environment for students and faculty members.
The Complexity of the Arterial Blood Pressure Regulation during the Stress Test
Authors: Qammar Naseha Wafa , Orinaitė Ugnė , Šiaučiūnaitė Vaiva , Vainoras Alfonsas , Šakalytė Gintarė , Ragulskis Minvydas
Published: May 2022

In this study, two categories of persons with normal and high ABP are subjected to the bicycle stress test (9 persons with normal ABP and 10 persons with high ABP). All persons are physically active men but not professional sportsmen. The mean and the standard deviation of age is 41.11 ± 10.21 years; height 178.88 ± 0.071 m; weight 80.53 ± 10.01 kg; body mass index 25.10 ± 2.06 kg/m2. Machine learning algorithms are employed to build a set of rules for the classification of the performance during the stress test. The heart rate, the JT interval, and the blood pressure readings are observed during the load and the recovery phases of the exercise. Although it is obvious that the two groups of persons will behave differently throughout the bicycle stress test, with this novel study, we are able to detect subtle variations in the rate at which these changes occur. This paper proves that these differences are measurable and substantial to detect subtle differences in the self-organization of the human cardiovascular system. It is shown that the data collected during the load phase of the stress test plays a more significant role than the data collected during the recovery phase. The data collected from the two groups of persons are approximated by Gaussian distribution. The introduced classification algorithm based on the statistical analysis and the triangle coordinate system helps to determine whether the reaction of the cardiovascular system of a new candidate is more pronounced by an increased heart rate or an increased blood pressure during the stress test. The developed approach produces valuable information about the self-organization of human cardiovascular system during a physical exercise.
Concentrated Growth Factors (CGF) Combined with Melatonin in Guided Bone Regeneration (GBR): A Case Report
Authors: Leonida Alessandro , Favero Gaia , Caccianiga Paolo , Ceraulo Saverio , Rodella Luigi Fabrizio , Rezzani Rita , Caccianiga Gianluigi
Published: May 2022

During implant restorative dentistry, common and crippling postoperative complications are pain and swelling of perioral soft tissues which engraving on patient quality of life. Concentrated growth factors (CGF), a novel generation of autologous platelet concentrate, and melatonin, endogenous indoleamine with also bone regenerative properties, may be useful for reconstruction of bony defects as well as in prosthetic and esthetic rehabilitation. We report a clinical case in which guided bone regeneration was performed combining CGF, melatonin and heterologous biomaterial. Great postoperative recovery without any complications was reported. In conclusion, in restorative dentistry the combined use of CGF and melatonin may have important roles in restoring bone defect, in improving implant osteointegration and, not less important, in preventing postoperative complications.
Responsive Teaching and the Instructional Reasoning of Expert Elementary Mathematics Teachers
Authors: Lindstrom Denise , Selmer Sarah
Published: May 2022

This study examines instructional reasoning in an approximation of practice that simulates a teacher sitting down after class to examine students’ written work. The participants were prompted to attend to, interpret, and decide how to respond to student thinking contained in a piece of written work. Our purpose was to capture the additional cognitive work that teachers engage in. Using qualitative content analysis, we identified the most frequent types of instructional reasoning used by expert teachers just prior to engaging in a responsive deciding action about how to respond. We used the results of our analysis to present three illustrative cases of responsive instructional reasoning.
A Theory-Led Evaluation of a Scalable Intervention to Promote Evidence-Based, Research-Informed Practice in Schools to Address Attainment Gaps
Authors: Hofmann Riikka , Ilie Sonia
Published: May 2022

Evidence-based practice is a salient solution that has been presented to address the persistent educational attainment gap linked to economic disadvantage. However, most schools do not engage with research, and we know little about facilitating school-led research use at scale. Linking different approaches to studying educational effectiveness, equity and change, and drawing on cultural-historical activity theory, this study develops intermediate theory about the mechanisms influencing institutions’ success in using research. In the context of the Opportunity Area Programme, supporting place-based interventions in the UK’s most socio-economically disadvantaged regions, we conducted a theory-led evaluation of the Evidence-Based Practice Fund (EBPF), aimed at supporting school-led research engagement to improve learning outcomes. We analysed the documentation of 83 EBPF projects, 8 focus groups, and a school survey. We demonstrate that enabling schools to address locally identified needs motivates research engagement but formulating these as stimulus for change requires scaffolding. Schools were keen but needed re-translation to use research to address those challenges. Low-key school-to-school support was found adequate. Leadership support and collaboration were significant but require relational expertise and professional learning to be effective. This study demonstrates that the use of research by schools at scale is possible and can transform a school’s agency in developing their own practice to improve equity.