bluuebunny/crossref_metadata_2025 huggingface dataset:
Sistem Pengambilan Keputusan Template Website Berbasis Pembobotan Adiktif Sederhana
Authors: Aminuddin Rosihan , Syarif Irwan , Rais Muhammad , Taha Syamsumarlin
Published: December 2023

Pada sebuah situs web merupakan salah satu faktor kunci untuk mendapatkan hasil penilaian yang positif dari pengunjung website, karena dapat mempengaruhi persepsi dan perilaku pengunjung terhadap situs web tersebut. Saat ini, banyak sekali yang menawarkan berbagai macam pilihan template desain website yang dapat langsung digunakan. Akan tetapi, hal tersebut justru dapat membuat orang-orang semakin kebingungan dan kesulitan untuk mengambil keputusan template mana yang akan digunakan. Oleh karena itu, diperlukan adanya sistem yang mampu memberikan rekomendasi template desain website yang sesuai dengan keinginan dari pengguna. Sistem tersebut adalah sistem rekomendasi pemilihan template desain website. Sistem ini membutuhkan masukan berupa preferensi dari pengguna tentang atribut desain website yang diinginkannya. Data preferensi tersebutlah yang akan digunakan sebagai acuan untuk memberikan rekomendasi template desain website kepada pengguna. Selain data tersebut sistem rekomendasi juga membutuhkan metode perhitungan tertentu untuk digunakan sebagai perhitungan agar dapat menghasilkan rekomendasi template desain website yang tepat sesuai dengan keinginan pengguna. Dalam penelitian ini, dibuat sistem rekomendasi pemilihan template desain website menggunakan metode perhitungan weighted sum model. Dari hasil penelitian ini, dihasilkan tingkat persentase recognition rate adalah sebesar 87% dan persentase tingkat error rate adalah sebesar 13%. Hal tersebut membuktikan bahwa metode weighted sum model mampu memberikan rekomendasi template desain website sesuai dengan preferensi yang telah diberikan oleh pengguna.
Anger Level of Patients in Outpatient Waiting Area and Related Factors
Authors: Ozturk Nezihenur , Aydin Muhammet Rasit , Aydin Abdulkadir , Ekerbicer Hasan Cetin
Published: December 2023

Aim: In this study, we aimed to detect the anger level of patients in the outpatient waiting area and related factors. We also aimed to contribute to the studies to be carried out to prevent violence in health caused by uncontrolled anger.

Methods: Volunteer patients aged 18 and over who applied to outpatient clinics were included in the study. A survey consisting of 34 questions prepared by the researchers and Spielberger's State Anger Scale consisting of 10 questions were used.

Results: In our study, it was detected that there was a statistically significant increase in state anger scale scores of patients who resorted to violence in health before, who are troubled with the priority patient practice, who cannot communicate with the doctor adequately, who cannot sit in the outpatient waiting area and those who wait for a long time, who have trouble making an appointment, who smoke and who have low income and education level.

Conclusion: We believe that establishing effective communication between health professionals and patients, increasing the number of healthcare workers, organizing work environments, and creating an efficient appointment system will contribute to a decrease in patient anger and a reduction in violence in healthcare.

Keywords: outpatient health services, anger, violence
Thymic Carcinoma: Unraveling Neuroendocrine Differentiation and Epithelial Cell Identity Loss
Authors: Yamada Yosuke , Iwane Kosuke , Nakanishi Yuki , Haga Hironori
Published: December 2023

Background: The histogenesis of thymic epithelial tumors (TETs) has been a subject of debate. Recent technological advancements have revealed that thymic carcinomas often exhibit a phenotype akin to tuft cells, which is a subset of medullary TECs. Here, we further explored the gene expression signatures of thymic carcinomas in relation to tuft cells and their kinships—ionocytes and neuroendocrine cells (neuroendocrine group). Methods: We analyzed a single-cell RNA sequencing dataset from the normal human thymus. Concurrently, we examined publicly available datasets on the mRNA expression and methylation status of TECs and lung cancers. Real-time quantitative PCR was also conducted with our tissue samples. Results: Thymic carcinomas displayed a neuroendocrine phenotype biased toward tuft cells and ionocytes. When exploring the possible regulators of this phenotype, we discovered that HDAC9 and NFATC1 were characteristically expressed in the neuroendocrine group in adult TECs and thymic carcinomas. Additionally, the pan-thymic epithelium markers, exemplified by PAX9 and SIX1, were significantly suppressed in thymic carcinomas. Conclusions: Thymic carcinomas might be characterized by unique neuroendocrine differentiation and loss of identity as thymic epithelial cells. Future studies investigating the role of HDAC9 and NFATC1 in thymic epithelium are warranted to explore their potential as therapeutic targets in TETs.
The Role of Receptor–Ligand Interaction in Somatostatin Signaling Pathways: Implications for Neuroendocrine Tumors
Authors: Milewska-Kranc Agnieszka , Ćwikła Jarosław B. , Kolasinska-Ćwikła Agnieszka
Published: December 2023

Neuroendocrine tumors (NETs) arise from neuroendocrine cells and manifest in diverse organs. Key players in their regulation are somatostatin and its receptors (SSTR1–SSTR5). Understanding receptor–ligand interactions and signaling pathways is vital for elucidating their role in tumor development and therapeutic potential. This review highlights SSTR characteristics, localization, and expression in tissues, impacting physiological functions. Mechanisms of somatostatin and synthetic analogue binding to SSTRs, their selectivity, and their affinity were analyzed. Upon activation, somatostatin initiates intricate intracellular signaling, involving cAMP, PLC, and MAP kinases and influencing growth, differentiation, survival, and hormone secretion in NETs. This review explores SSTR expression in different tumor types, examining receptor activation effects on cancer cells. SSTRs’ significance as therapeutic targets is discussed. Additionally, somatostatin and analogues’ role in hormone secretion regulation, tumor growth, and survival is emphasized, presenting relevant therapeutic examples. In conclusion, this review advances the knowledge of receptor–ligand interactions and signaling pathways in somatostatin receptors, with potential for improved neuroendocrine tumor treatments.
Clinical Outcomes of HER2-Negative Metastatic Breast Cancer Patients in Italy in the Last Decade: Results of the GIM 13-AMBRA Study
Authors: Cazzaniga Marina Elena , Pronzato Paolo , Amoroso Domenico , Bernardo Antonio , Biganzoli Laura , Bisagni Giancarlo , Blasi Livio , Bria Emilio , Cognetti Francesco , Crinò Lucio , De Laurentiis Michelino , Del Mastro Lucia , De Placido Sabino , Beano Alessandra , Ferraù Francesco , Foladore Silva , Forcignanò Rosachiara , Gamucci Teresa , Garrone Ornella , Gennari Alessandra , Giordano Monica , Giotta Francesco , Giovanardi Filippo , Latini Luciano , Livi Lorenzo , Marchetti Paolo , Mattioli Rodolfo , Michelotti Andrea , Montemurro Filippo , Putzu Carlo , Riccardi Ferdinando , Ricciardi Giuseppina , Romagnoli Emanuela , Sarobba Giuseppina , Spazzapan Simon , Tagliaferri Pierosandro , Tinari Nicola , Tonini Giuseppe , Turletti Anna , Verusio Claudio , Zambelli Alberto , Mustacchi Giorgio
Published: December 2023

GIM 13-AMBRA is a longitudinal cohort study aimed at describing therapeutic strategies and the relative outcome parameters in 939 HER2-ve MBC patients. Taxanes–based regimens, or taxanes + targeted agents, mainly Bevacizumab, were the preferred first choice in both Luminal (30.2%) and TNBC (33.3%) patients. The median PFS1 was 12.5 months (95% CI 16.79–19.64), without any significant difference according to subtypes, while the median Time to first Treatment Change (TTC1) was significantly lower in TNBC patients (7.7 months—95% CI 5.7–9.2) in comparison to Luminal A (13.2 months, 95% CI 11.7–15.1) and Luminal B patients (11.8 months, 95% CI 10.3–12.8). PFS2 was significantly shorter in TNBC patients (5.5 months, 95% CI 4.3–6.5 vs. Luminal A—9.4, 95% CI 8.1–10.7, and Luminal B—7.7 95% CI 6.8–8.2, F-Ratio 4.30, p = 0.014). TTC2 was significantly lower in patients with TNBC than in those with the other two subtypes. The median OS1 was 35.2 months (95% CI 30.8–37.4) for Luminal A patients, which was significantly higher than that for both Luminal B (28.9 months, 95% CI 26.2–31.2) and TNBC (18.5 months, 95% CI 16–20.1, F-ratio 7.44, p = 0.0006). The GIM 13—AMBRA study is one of the largest collections ever published in Italy and provides useful results in terms of time outcomes for first, second, and further lines of treatment in HER2- MBC patients.
Lymphovascular Invasion at the Time of Radical Prostatectomy Adversely Impacts Oncological Outcomes
Authors: Sathianathen Niranjan J. , Furrer Marc A. , Mulholland Clancy J. , Katsios Andreas , Soliman Christopher , Lawrentschuk Nathan , Peters Justin S. , Zargar Homi , Costello Anthony J. , Hovens Christopher M. , Bishop Conrad , Rao Ranjit , Tong Raymond , Steiner Daniel , Moon Daniel , Thomas Benjamin C. , Dundee Philip , Calero Jose Antonio Rodriguez , Thalmann George N. , Corcoran Niall M.
Published: December 2023

Lymphovascular invasion, whereby tumour cells or cell clusters are identified in the lumen of lymphatic or blood vessels, is thought to be an essential step in disease dissemination. It has been established as an independent negative prognostic indicator in a range of cancers. We therefore aimed to assess the impact of lymphovascular invasion at the time of prostatectomy on oncological outcomes. We performed a multicentre, retrospective cohort study of 3495 men who underwent radical prostatectomy for localised prostate cancer. Only men with negative preoperative staging were included. We assessed the relationship between lymphovascular invasion and adverse pathological features using multivariable logistic regression models. Kaplan–Meier curves and Cox proportional hazard models were created to evaluate the impact of lymphovascular invasion on oncological outcomes. Lymphovascular invasion was identified in 19% (n = 653) of men undergoing prostatectomy. There was an increased incidence of lymphovascular invasion-positive disease in men with high International Society of Urological Pathology (ISUP) grade and non-organ-confined disease (p < 0.01). The presence of lymphovascular invasion significantly increased the likelihood of pathological node-positive disease on multivariable logistic regression analysis (OR 15, 95%CI 9.7–23.6). The presence of lymphovascular invasion at radical prostatectomy significantly increased the risk of biochemical recurrence (HR 2.0, 95%CI 1.6–2.4). Furthermore, lymphovascular invasion significantly increased the risk of metastasis in the whole cohort (HR 2.2, 95%CI 1.6–3.0). The same relationship was seen across D’Amico risk groups. The presence of lymphovascular invasion at the time of radical prostatectomy is associated with aggressive prostate cancer disease features and is an indicator of poor oncological prognosis.
Towards Blockchain-Integrated Enterprise Resource Planning: A Pre-Implementation Guide
Authors: Imane Lahlou , Noureddine Motaki , Driss Sarsri , Hanane L’yarfi
Published: December 2023

In the face of numerous challenges in supply chain management, new technologies are being implemented to overcome obstacles and improve overall performance. Among these technologies, blockchain, a part of the distributed ledger family, offers several advantages when integrated with ERP systems, such as transparency, traceability, and data security. However, blockchain remains a novel, complex, and costly technology. The purpose of this paper is to guide decision-makers in determining whether integrating blockchain technology with ERP systems is appropriate during the pre-implementation phase. This paper focuses on the literature reviews, theories, and expert opinions to achieve its objectives. It first provides an overview of blockchain technology, then discusses its potential benefits to the supply chain, and finally proposes a framework to assist decision-makers in determining whether blockchain meets the needs of their consortium and whether this integration aligns with available resources. The results highlight the complexity of blockchain, the importance of detailed and in-depth research in deciding whether to integrate blockchain technology into ERP systems, and future research prospects. The findings of this article also present the critical decisions to be made prior to the implementation of blockchain, in the event that decision-makers choose to proceed with blockchain integration. The findings of this article augment the existing literature and can be applied in real-world contexts by stakeholders involved in blockchain integration projects with ERP systems.
The Velamen Radicum Is Common in the Genus Anthurium, Both in the Epiphytic and Terrestrial Species
Authors: Werner Julia C. , Albach Dirk C. , Can Levent , Zotz Gerhard
Published: December 2023

The velamen radicum, a rhizodermis that consists of dead cells at maturity, is often described as typical for epiphytic aroids. Such claims are surprising on two grounds: (1) there are hardly any data on this trait for aroids and (2) the link between a velamen and epiphytic growth has recently been challenged in general. We performed an anatomical and histological study with 82 Anthurium species and analyzed the occurrence of a velamen in regard to habit (epiphytic vs. terrestrial) and phylogenetic relatedness. Almost 90% of both epiphytic and terrestrial species had a velamen. The number of cell layers comprising this tissue were also very similar in both groups. The most likely interpretation of the phylogenetic tree suggests that a velamen is not ancestral in Anthurium. It was gained once and has been lost several times during diversification of the genus. Our results are an important contribution to the current discussion on the possible function of the velamen. While there is some experimental evidence for its importance for epiphytic plants, its role in terrestrial plants is completely unresolved.
A Case Study on the CO2 Sequestration in Shenhua Block Reservoir: The Impacts of Injection Rates and Modes
Authors: Tang Ligen , Ding Guosheng , Song Shijie , Wang Huimin , Xie Wuqiang , Wang Jiulong
Published: December 2023

Carbon capture and storage (CCS) is the most promising method of curbing atmospheric carbon dioxide levels from 2020 to 2050. Accurate predictions of geology and sealing capabilities play a key role in the safe execution of CCS projects. However, popular forecasting methods often oversimplify the process and fail to guide actual CCS projects in the right direction. This study takes a specific block in Shenhua, China as an example. The relative permeability of CO2 and brine is measured experimentally, and a multi-field coupling CO2 storage prediction model is constructed, focusing on analyzing the sealing ability of the block from the perspective of injection modes. The results show that when injected at a constant speed, the average formation pressure and wellbore pressure are positively correlated with the CO2 injection rate and time; when the injection rate is 0.5 kg/s for 50 years, the average formation pressure increases by 38% and the wellbore pressure increases by 68%. For different injection modes, the average formation pressures of various injection methods are similar during injection. Among them, the pressure increases around the well in the decreasing injection mode is the smallest. The CO2 concentration around the wellbore is the largest, and the CO2 diffusion range continues to expand with injection time. In summary, formation pressure increases with the increase in injection rate and injection time, and the decreasing injection mode has the least impact on the increase in formation pressure. The CO2 concentration is the largest around the well, and the CO2 concentration gradually decreases. The conclusion helps determine the geological carrying capacity of injection volumes and provides insights into the selection of more appropriate injection modes. Accurate predictions of CO2 storage capacity are critical to ensuring project safety and monitoring potentially hazardous sites based on reservoir characteristics.
Optimal Unit Commitment and Generation Scheduling of Integrated Power System with Plug-In Electric Vehicles and Renewable Energy Sources
Authors: Kamboj Vikram Kumar , Malik Om Parkash
Published: December 2023

The integration of wind energy sources and plug-in electric vehicles is essential for the efficient planning, reliability, and operation of modern electric power systems. Minimizing the overall operational cost of integrated power systems while dealing with wind energy sources and plug-in electric vehicles in integrated power systems using a chaotic zebra optimization algorithm (CZOA) is described. The proposed system deals with a probabilistic forecasting system for wind power generation and a realistic plug-in electric vehicle charging profile based on travel patterns and infrastructure characteristics. The objective is to identify the optimal scheduling and committed status of the generating unit for thermal and wind power generation while considering the system power demand, charging, and discharging of electric vehicles, as well as power available from wind energy sources. The proposed CZOA adeptly tackles the intricacies of the unit commitment problem by seamlessly integrating scheduling and the unit’s committed status, thereby enabling highly effective optimization. The proposed algorithm is tested for 10-, 20-, and 40-generating unit systems. The empirical findings pertaining to the 10-unit system indicate that the amalgamation of a thermal generating unit system with plug-in electric vehicles yields a 0.84% reduction in total generation cost. Furthermore, integrating the same system with a wind energy source results in a substantial 12.71% cost saving. Notably, the integration of the thermal generating system with both plug-in electric vehicles and a wind energy source leads to an even more pronounced overall cost reduction of 13.05%. The outcome of this study reveals competitive test results for 20- and 40-generating unit systems and contributes to the advancement of sustainable and reliable power systems, fostering the transition towards a greener energy future.
Cost-Effective Optimization of an Array of Wave Energy Converters in Front of a Vertical Seawall
Authors: Natarajan Senthil Kumar , Cho Il Hyoung
Published: December 2023

The present paper focuses on investigating the cost-effective configuration of an array of wave energy converters (WECs) composed of vertical cylinders situated in front of a vertical seawall in irregular waves. First, the hydrodynamic calculations are performed using a WAMIT commercial code based on linear potential theory, where the influence of the vertical wall is incorporated using the method of image. The viscous damping experienced by the oscillating cylinder is considered through CFD simulations of a free decay test. A variety of parameters, including WEC diameter, number of WECs, and the spacing between them, are considered to determine an economically efficient WEC configuration. The design of the WEC configuration is aided by a cost indicator, defined as the ratio of the total submerged volume of the WEC to overall power capture. The cost-effective configuration of WECs is achieved when WECs are positioned in front of a vertical wall and the distance between them is kept short. It can be explained that the trapped waves formed between adjacent WECs as well as the standing waves in front of a seawall significantly intensify wave fields around WECs and consequently amplify the heave motion of each WEC. A cost-effective design strategy of WEC deployment enhances the wave energy greatly and, consequently, contributes to constructing the wave energy farm.
Large-Scale Hydrogen Production Systems Using Marine Renewable Energies: State-of-the-Art
Authors: Ngando Ebba Junior Diamant , Camara Mamadou Baïlo , Doumbia Mamadou Lamine , Dakyo Brayima , Song-Manguelle Joseph
Published: December 2023

To achieve a more ecologically friendly energy transition by the year 2050 under the European “green” accord, hydrogen has recently gained significant scientific interest due to its efficiency as an energy carrier. This paper focuses on large-scale hydrogen production systems based on marine renewable-energy-based wind turbines and tidal turbines. The paper reviews the different technologies of hydrogen production using water electrolyzers, energy storage unit base hydrogen vectors, and fuel cells (FC). The focus is on large-scale hydrogen production systems using marine renewable energies. This study compares electrolyzers, energy storage units, and FC technologies, with the main factors considered being cost, sustainability, and efficiency. Furthermore, a review of aging models of electrolyzers and FCs based on electrical circuit models is drawn from the literature and presented, including characterization methods of the model components and the parameters extraction methods, using a dynamic current profile. In addition, industrial projects for producing hydrogen from renewable energies that have already been completed or are now in progress are examined. The paper is concluded through a summary of recent hydrogen production and energy storage advances, as well as some applications. Perspectives on enhancing the sustainability and efficiency of hydrogen production systems are also proposed and discussed. This paper provides a review of behavioral aging models of electrolyzers and FCs when integrated into hydrogen production systems, as this is crucial for their successful deployment in an ever-changing energy context. We also review the EU’s potential for renewable energy analysis. In summary, this study provides valuable information for research and industry stakeholders aiming to promote a sustainable and environmentally friendly energy transition.
Characteristic Quantity Analysis of Single-Phase Contact Tree Ground Fault of Distribution Network Overhead Lines
Authors: He Jianbo , Zhou Yao , Li Yilin , Zhang Guangqing , Liang Jiayu , Shang Hao , Ning Wenjun
Published: December 2023

When the overhead line passes through the forest area, the conductor may contact the line to induce the tree-contact single-phase-to-ground fault (TSF), and the persistence of TSF may induce wildfires, bringing serious consequences. However, the amount of TSF electrical features is weak, and traditional protection devices cannot operate effectively, so it is urgent to obtain typical characteristics of TSF. In this study, the simulation experiment is carried out for the tree-contact single-phase-to-ground fault. Firstly, the relativity between fault and characteristics like zero-sequence voltage, zero-sequence current, and differential current are analyzed theoretically. Then, the simulation experiment platform of TSF is built, and the time-varying fault characteristics are acquired. The experimental results show that the average value of the zero-sequence voltage, the amplitude of the power-frequency component of the zero-sequence current, and the amplitude of the power-frequency component of the first and end differential current can accurately reflect the fault current development trend of the single-phase contact tree fault of the conductor, and can be used as the typical characteristic quantity of TSF. The results of this study are helpful for further understanding the fault characteristics of TSF and provide theoretical support for the identification and protection design of TSF.
The Future of Flying Base Stations: Empirical and Numerical Investigations of mmWave-Enabled UAVs
Authors: Masaoka Ryunosuke , Tran Gia Khanh , Nakazato Jin , Sakaguchi Kei
Published: December 2023

Nowadays, wireless communications are ubiquitously available. However, as pervasive as this technology is, there are distinct situations, such as during substantial public events, catastrophic disasters, or unexpected malfunctions of base stations (BSs), where the reliability of these communications might be jeopardized. Such scenarios highlight the vulnerabilities inherent in our current infrastructure. As a result, there is growing interest in establishing temporary networks that offer high-capacity communications and can adaptively shift service locations. To address this gap, this paper investigates the promising avenue of merging two powerful technologies: Unmanned Aerial Vehicles (UAVs) and millimeter-wave (mmWave) transmissions. UAVs, with their ability to be operated remotely and to take flight without being constrained by terrestrial limitations, present a compelling case for being the cellular BSs of the future. When integrated with the high-speed data transfer capabilities of mmWave technology, the potential is boundless. We embark on a hands-on approach to provide a tangible foundation for our hypothesis. We carry out comprehensive experiments using an actual UAV equipped with an mmWave device. Our main objective is to meticulously study its radio wave propagation attributes when the UAVs are in flight mode. The insights gleaned from this hands-on experimentation are profound. We contrast our experimental findings with a rigorous numerical analysis to refine our understanding. This comparative study aimed to shed light on the intricacies of wave propagation behaviors within the vast expanse of the atmosphere.
Design and Implementation of a Digital Twin System for Log Rotary Cutting Optimization
Authors: Zhao Yadi , Yan Lei , Wu Jian , Song Ximing
Published: December 2023

To address the low level of intelligence and low utilization of logs in current rotary cutting equipment, this paper proposes a digital twin-based system for optimizing the rotary cutting of logs using a five-dimensional model of digital twins. The system features a log perception platform to capture three-dimensional point cloud data, outlining the logs’ contours. Utilizing the Delaunay3D algorithm, this model performs a three-dimensional reconstruction of the log point cloud, constructing a precise digital twin. Feature information is extracted from the point cloud using the least squares method. Processing parameters, determined through the kinematic model, are verified in rotary cutting simulations via Bool operations. The system’s efficacy has been substantiated through experimental validation, demonstrating its capability to output specific processing schemes for irregular logs and to verify these through simulation. This approach notably improves log recovery rates, decreasing volume error from 12.8% to 2.7% and recovery rate error from 23.5% to 5.7% The results validate the efficacy of the proposed digital twin system in optimizing the rotary cutting process, demonstrating its capability not only to enhance the utilization rate of log resources but also to improve the economic efficiency of the factory, thereby facilitating industrial development.
Machine Learning to Advance Human Genome-Wide Association Studies
Authors: Sigala Rafaella E. , Lagou Vasiliki , Shmeliov Aleksey , Atito Sara , Kouchaki Samaneh , Awais Muhammad , Prokopenko Inga , Mahdi Adam , Demirkan Ayse
Published: December 2023

Machine learning, including deep learning, reinforcement learning, and generative artificial intelligence are revolutionising every area of our lives when data are made available. With the help of these methods, we can decipher information from larger datasets while addressing the complex nature of biological systems in a more efficient way. Although machine learning methods have been introduced to human genetic epidemiological research as early as 2004, those were never used to their full capacity. In this review, we outline some of the main applications of machine learning to assigning human genetic loci to health outcomes. We summarise widely used methods and discuss their advantages and challenges. We also identify several tools, such as Combi, GenNet, and GMSTool, specifically designed to integrate these methods for hypothesis-free analysis of genetic variation data. We elaborate on the additional value and limitations of these tools from a geneticist’s perspective. Finally, we discuss the fast-moving field of foundation models and large multi-modal omics biobank initiatives.
The Effects of Nine Compounds on Aldehyde-Oxidase-Related Genes in Bactrocera dorsalis (Hendel)
Authors: Zou Yan , Chen Yupeng , Wang Duoduo , Xie Xiaowei , Li Gen , Zheng Chunyan , Wen Jian , Su Hongai , Liu Xin , Zeng Ling , Lu Yongyue , Cao Fengqin
Published: December 2023

Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) (B. dorsalis) is an important agricultural, major invasive, and quarantine pest that can cause significant damage to the economic value of the fruit and vegetable industry. Male bait is one of the most effective methods of surveying, monitoring, and controlling B. dorsalis. In our study, we constructed cDNA libraries using total RNA extracted independently from the antennae, mouthparts, and thoracic legs of male and female adults and the ovipositors of female adults and screened out four aldehyde-oxidase-related genes (AOX-related), C58800, C66700, C67485, and C67698. Molecular docking predictions showed that eight compounds, including 3,4-dimethoxycinnamyl alcohol, 3,4-dimethoxy-cinnamaldehyde, deet, ethyl N-acetyl-N-butyl-β-alaninate, n-butyl butyrate, n-butyl butyrate, ethyl butyrate, methyl eugenol, and ethyl acetate, could combine with proteins encoded by the four B. dorsalis AOX-related genes. Furthermore, QPCR was performed to confirm that four compounds, including 3,4-dimethoxy cinnamic aldehyde, butyl levulinic acid ethyl ester (mosquito repellent), butyl butyrate, and methyl eugenol, induced significant changes in the AOX-related genes of B. dorsalis. These results provide useful information and guidance for the batch screening of potentially useful compounds and the search for effective attractants of B. dorsalis.
Story Luminary Phyllis (Jack) Webstad and the Storywork of the Orange Shirt
Authors: Wurfel Marlene
Published: December 2023

An orange shirt is synonymous with truth and reconciliation in Canada. How did this symbol spread from a personal story about surviving a residential school, to a children’s book by author Phyllis J. Webstad, to a national symbol of Indigenous solidarity and allyship? This paper examines the work of Webstad as a Canadian story luminary, using historical and textual analysis to explore the power of “The Orange Shirt Story” to decolonize, resist, refuse, and transform. The orange shirt reveals the deep connectedness between storytelling, social justice, resilience, and activism.
Digital Tools for Data Acquisition and Heritage Management in Archaeology and Their Impact on Archaeological Practices
Authors: Moullou Dorina , Vital Rebeka , Sylaiou Stella , Ragia Lemonia
Published: December 2023

The significance of data acquisition in archaeological practice has consistently held great importance. Over the past few decades, the growing prevalence of digitization in acquiring data has significantly transformed the landscape of archaeological fieldwork, influencing both methodology and interpretation. The integration of digital photogrammetry and laser scanning technologies in archaeology has transformed data acquisition, enabling efficient and precise documentation. However, this digital shift raises concerns about information overload, the potential loss of on-site insights, and the need for suitable data management methods. Over the past 15 years, digital tools like photogrammetry, laser scanning, and unmanned aerial vehicles have advanced cultural heritage documentation. These methods offer detailed 3D models of archaeological sites, artifacts, and monuments, with evolving accessibility and user friendliness. This paper delves into methods for documenting cultural heritage, examining the implications of various approaches on the archaeologist’s workflow and on the field as a whole.
Navigating Mental Health: Community Members’ Insights into Social Support and Inclusion in Matsafeni Village in Mbombela, Mpumalanga Province—A Qualitative Study
Authors: Mboweni Nkhensani Eseldah , Mphasha Mabitsela Hezekiel , Skaal Linda
Published: December 2023

Mental health illnesses are increasingly prevalent worldwide, and South Africa is no exception. The research investigates the vital significance of social support in enhancing the welfare of individuals dealing with mental disorders. An essential aspect involves comprehending the interplay of emotional and practical supports provided by both families and the larger community. This study aims to explore community perceptions regarding social support and the involvement of individuals diagnosed with mental illnesses in Matsafeni Village. The research employed the qualitative method and descriptive exploratory research design, enabling the researchers to explore social support and the involvement of individuals with mental health disorders. Data were collected through unstructured, open-ended interviews, and participants were selected using a convenience sampling method. A total of only 15 participants were enrolled in this study, with variations in their educational backgrounds. Notably, only two participants had firsthand experiences with family members facing mental disorders. The study underscored the critical roles of family and community supports for individuals diagnosed with mental health disorders. The participants emphasized the necessity of mentally ill individuals receiving support from their loved ones and the broader community. Furthermore, they highlighted the importance for including individuals with mental health disorders in community activities as a means of better managing their conditions. The findings of this study can serve as a foundation for developing interventions to assist and support individuals affected by mental health disorders. Policymakers can also utilize the information to formulate strategies and best practices for promoting mental health awareness within the community.
Young Adults at the National Epicenter of the COVID-19 Outbreak: Understanding the Impact and Future Challenges of Social Distancing on Mental Health Outcomes
Authors: Malik Sana , Opara Ijeoma , Lardier David T. , Younger Jessica , Greene R. Neil
Published: December 2023

Objectives. To understand the role and future implications of social distancing on mental health and substance use in young adults between the ages of 18 and 35 living in high-disease-prevalent areas of New York. Methods. Participants completed a self-administered online survey through Qualtrics. Results. 43.9% of the sample met criteria for moderate or severe anxiety, and 53.1% of the sample met criteria for moderate to severe depression. 76.1% of the sample experienced both anxiety and depressive symptoms. Despite this, the rates of lifetime mental health diagnoses, treatment, and access to mental health services were low. Rates of depression and anxiety differed across socio-demographic variables (gender, income, sexuality, education, and insurance status). Experiencing severe symptoms of the COVID-19 virus, poor coping skills, loneliness, increased alcohol use, and sleep disturbances were linked to higher rates of depression, anxiety, or both. Conclusion. As the first epicenter of COVID-19 in the United States, New York represents an important location for prevention researchers to learn about how COVID-19 affected a diverse population of young adults. It is essential that researchers and practitioners proactively develop early and appropriate interventions to address the ongoing mental health crisis and also plan for future crises.
Nickel Challenge In Vitro Affects CD38 and HLA-DR Expression in T Cell Subpopulations from the Blood of Patients with Nickel Allergy
Authors: Artuc Metin , Zuberbier Torsten , Peiser Matthias
Published: December 2023

Nickel allergy is a major health problem and shows clinical manifestation of contact eczema. The response of specific lymphocyte subpopulations in sensitized patients after new challenge to nickel has until now not been studied in detail. To evaluate if nickel-based elicitation reaction could be objectively identified by multi-parametric flow cytometry, immunophenotyping of specific T cells was applied. White blood cells from 7 patients (4 positive in patch test, 3 negative) were challenged by nickel and in vitro short-term culture. Standardized antibody-dye combinations, specific for T helper(h)1, Th17 and cytotoxic T cell activation, were selected according to the recommendations of Stanford Human Immune Monitoring Center. In cytotoxic CD8+CCR7+CD45RA+ T cells from patients suffering from nickel allergy, CD38 and HLA-DR were elevated comparing to healthy donors. After challenge to nickel in vitro both markers decreased in CD8+CCR7+CD45RA+ T cells but found up-regulated in CD4+CCR7+CD45RA+CCR6−CXCR3+Th1 cells. Intracellular expression of T-bet and RORγt further indicated Th1 and Th17 cells. Finally, CD4+CD25+CCR4− T cells increased after challenge with nickel in PBMCs of patients with nickel allergy. Flow cytometry based quantification of T cell markers might be used as a specific and reliable method to detect chemical induced skin sensitization and confirm diagnostic patch testing in the clinics.
Madurastatins with Imidazolidinone Rings: Natural Products or Side-Reaction Products from Extraction Solvents?
Authors: Pérez-Bonilla Mercedes , Sánchez-Hidalgo Marina , González Ignacio , Oves-Costales Daniel , Martín Jesús , Murillo-Alba José , Tormo José R. , Cho Ahreum , Byun Soo-Young , No Joo-Hwan , Shum David , Ioset Jean-Robert , Genilloud Olga , Reyes Fernando
Published: December 2023

Madurastatins are a group of pentapeptides containing an oxazoline moiety, and, in a few cases, an imidazolidinone ring as an additional structural feature. In our search for new potential antiparasitic metabolites from natural sources, we studied the acetone extracts from a culture of Actinomadura sp. CA-135719. The LC/HRMS analysis of this extract identified the presence of the known madurastatins C1 (1), D1 (4), and D2 (5) together with additional members of the family that were identified as the new madurastatins H2 (2) and 33-epi-D1 (3) after isolation and spectroscopic analysis. The planar structures of the new compounds were established by HRMS, ESI-qTOF-MS/MS, and 1D and 2D NMR data, and their absolute configuration was proposed using Marfey’s and bioinformatic analyses of the biosynthetic gene cluster (BGC). A revision of the absolute configuration of madurastatins D1 and D2 is proposed. Additionally, madurastatins containing imidazolidinone rings are proved to be artifacts originating during acetone extraction of the bacterial cultures.
Spontaneous Attenuation of Alcoholic Fermentation via the Dysfunction of Cyc8p in Saccharomyces cerevisiae
Authors: Watanabe Daisuke , Kumano Maika , Sugimoto Yukiko , Takagi Hiroshi
Published: December 2023

A cell population characterized by the release of glucose repression and known as [GAR+] emerges spontaneously in the yeast Saccharomyces cerevisiae. This study revealed that the [GAR+] variants exhibit retarded alcoholic fermentation when glucose is the sole carbon source. To identify the key to the altered glucose response, the gene expression profile of [GAR+] cells was examined. Based on RNA-seq data, the [GAR+] status was linked to impaired function of the Cyc8p–Tup1p complex. Loss of Cyc8p led to a decrease in the initial rate of alcoholic fermentation under glucose-rich conditions via the inactivation of pyruvate decarboxylase, an enzyme unique to alcoholic fermentation. These results suggest that Cyc8p can become inactive to attenuate alcoholic fermentation. These findings may contribute to the elucidation of the mechanism of non-genetic heterogeneity in yeast alcoholic fermentation.
Multiple Genomic Alterations, Including a Novel AFF4::IRF1 Fusion Gene, in a Treatment-Refractory Blastic Plasmacytoid Dendritic-Cell Neoplasm: A Case Report and Literature Review
Authors: Sahin Yavuz , Wang Y. Lynn , Pei Jianming , Mansoor Nashwa , Styler Michael , Testa Joseph R. , Nejati Reza
Published: December 2023

Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare hematologic malignancy with an aggressive clinical course and poor prognosis. The genetic abnormalities in BPDCN are heterogeneous; therefore, its molecular pathogenesis and the prognostic importance of genomic alterations associated with the disease are not well defined. Here we report a case of BPDCN with a novel AFF4::IRF1 fusion predicted to lead to a loss-of-function of the IRF1 tumor suppressor, somatic mutations of ASXL1, TET2, and MYD88, as well as multiple intrachromosomal deletions. The patient showed resistance to Tagraxofusp and Venetoclax, and he died about 16 months after diagnosis. Considering the predicted effect of the AFF4::IRF1 fusion on IRF1’s antitumor effects and immune regulation, and the possibility of its relevance to the aggressive course observed in this case, we propose further evaluation of the clinical significance of this fusion in BPDCN in future cooperative group studies and the consideration of therapeutic strategies aimed at restoring IRF1-dependent antineoplastic effects in such cases.
Pathophysiology of Pre-Eclampsia—Two Theories of the Development of the Disease
Authors: Kornacki Jakub , Olejniczak Olga , Sibiak Rafał , Gutaj Paweł , Wender-Ożegowska Ewa
Published: December 2023

Pre-eclampsia (PE) continues to be a leading cause of maternal and fetal mortality and morbidity. While substantial progress has been made in understanding the pathomechanisms of PE, the pathophysiology of the disease is still not fully understood. While the “two-stage model” of the development of PE is the most widely accepted theory, stating that the placenta is the main source of the disease, there are some other pathophysiological models of PE. Among these other theories, the one considering heart dysfunction as serving as the primary cause of PE seems to be gaining increasing prominence. In this review, we aim to elucidate these two divergent concepts concerning the development of PE. Despite some differences in their proposed pathomechanisms, both theories share vital pathophysiological elements in common. A central and critical component in both models is impaired placental perfusion, which appears to be a crucial phenomenon in PE. A comprehensive understanding of the different pathomechanisms involved in PE may be helpful in clinical practice, prompting a more individual approach to care of patients with PE.
RETRACTED: Andrographolide Alleviates Oxidative Damage and Inhibits Apoptosis Induced by IHNV Infection via CTSK/BCL2/Cytc Axis
Authors: Liu Qi , Li Linfang , Zhao Jingzhuang , Ren Guangming , Lu Tongyan , Shao Yizhi , Xu Liming
Published: December 2023

Infectious hematopoietic necrosis virus (IHNV) is an important pathogen that causes significant economic losses to salmon trout farming. Although vaccines have been invented for the treatment of IHNV, findings from our previous survey show that breeding enterprises and farmers require effective oral drugs or immune enhancers. However, studies on the development of oral drugs are limited. In the present study, we used bioinformatics methods to predict the protein targets of andrographolide (Andro) in IHNV. Cells were infected with IHNV, and the effect of andrographolide was explored by evaluating the expression levels of genes implicated in oxidative stress, activities of antioxidant enzymes, and the expression of genes implicated in apoptosis and necrosis. In the present study, cells were divided into NC, IHNV, IHNV+10 μM andrographolide, and IHNV+20 μM andrographolide groups. qRT-PCR was performed to determine the expression level of genes, and an antioxidant enzyme detection kit was used to evaluate the activities of antioxidant enzymes. Fluorescent staining was performed using a reactive oxygen species detection kit (ROS) and Hoechst 33342/PI double staining kit, and the mechanism of alleviation of apoptosis and oxidative stress andrographolide after IHNV infection was determined. The results indicated that andrographolide inhibits viral growth by binding to the NV protein of IHNV and increasing the antioxidant capacity of the body through the CTSK/BCL2/Cytc axis, thereby inhibiting the occurrence of IHNV-induced apoptosis. This is the first study to explore the antagonistic mechanism of action of andrographolide in alleviating IHNV infection. The results provide valuable information on alternative strategies for the treatment of IHNV infection during salmon family and provide a reference for the use of andrographolide as an antioxidant agent in agricultural settings.
Metabolomic Analysis of the Effect of Freezing on Leaves of Malus sieversii (Ledeb.) M.Roem. Histoculture Seedlings
Authors: Su Yongfeng , Liu Lijun , Ma Hongxi , Yuan Yinyan , Zhang Deen , Lu Xiaoyan
Published: December 2023

Malus sieversii (Ledeb.) M.Roem. is the ancestor of cultivated apples, and is an excellent germplasm resource with high resistance to cold. Artificial refrigerators were used to simulate the low temperature of −3 °C to treat Malus sieversii (Ledeb.) M.Roem. histoculture seedlings. Observations were performed to find the effects of freezing stress on the status of open or closed stomata, photosystems, and detection of metabolomic products in leaves of Malus sieversii (Ledeb.) M.Roem. histoculture seedlings. The percentage of closed stomata in the Malus sieversii (Ledeb.) M.Roem. histoculture seedlings increased, the maximum fluorescence (Fm’) excited by a strong light (saturating pulse) was weakened relative to the real-time fluorescence in its vicinity, and the quantum yield of unregulated energy dissipation was increased in PSII under freezing stress. The metabolites in the leaves of the Malus sieversii (Ledeb. M.Roem.) histoculture seedlings were analyzed by ultra-performance liquid chromatography–tandem mass spectrometry using CK, T12h, T36 h, and HF24h. Results demonstrated that cold stress in the Malus sieversii (Ledeb.) M.Roem. histoculture seedlings led to wilting, leaf stomatal closure, and photosystem damage. There were 1020 metabolites identified as lipids (10.2%), nucleotides and their derivatives (5.2%), phenolic acids (19.12%), flavonoids (24.51%), amino acids and their derivatives (7.75%), alkaloids (5.39%), terpenoids (8.24%), lignans (3.04%), organic acids (5.88%), and tannins (0.88%). There were 110 differential metabolites at CKvsT12h, 113 differential metabolites at CKvsT36h, 87 differential metabolites at T12hvsT36h, 128 differential metabolites at CKvsHF24h, 121 differential metabolites at T12hvsHF24h, and 152 differential metabolites at T36hvsHF24h. The differential metabolites in the leaves of the Malus sieversii (Ledeb.) M.Roem. seedlings grown under low-temperature stress mainly involved glycolysis, amino acid metabolism, lipid metabolism, pyrimidine metabolism, purine metabolism, and secondary metabolite metabolism. The Malus sieversii (Ledeb.) M.Roem. seedlings responded to the freezing stress by coordinating with each other through these metabolic pathways. The metabolic network of the leaves of the Malus sieversii (Ledeb.) M.Roem. histoculture seedlings under low temperature stress was also proposed based on the above pathways to deepen understanding of the response of metabolites of Malus sieversii (Ledeb.) M.Roem. to low-temperature stress and to lay a theoretical foundation for the development and utilization of Malus sieversii (Ledeb.) M.Roem. cultivation resources.