bluuebunny/crossref_metadata_2025 huggingface dataset:
Buying Time with Runnels: a Climate Adaptation Tool for Salt Marshes
Authors: Besterman Alice F. , Jakuba Rachel W. , Ferguson Wenley , Brennan Diana , Costa Joseph E. , Deegan Linda A.
Published: January 2022

A prominent form of salt marsh loss is interior conversion to open water, driven by sea level rise in interaction with human activity and other stressors. Persistent inundation drowns vegetation and contributes to open water conversion in salt marsh interiors. Runnels are shallow channels originally developed in Australia to control mosquitoes by draining standing water, but recently used to restore marsh vegetation in the USA. Documentation on runnel efficacy is not widely available; yet over the past 10 years dozens of coastal adaptation projects in the northeastern USA have incorporated runnels. To better understand the efficacy of runnels used for restoration, we organized a workshop of 70 experts and stakeholders in coastal resource management. Through the workshop we developed a collective understanding of how runnels might be used to slow or reverse open water conversion, and identified unresolved questions. In this paper we present a synthesis of workshop discussions and results from a promising case study in which vegetation was restored at a degraded marsh within a few years of runnel construction. Despite case study outcomes, key questions remain on long-term runnel efficacy in marshes differing in elevation, tidal range, and management history. Runnel construction is unlikely to improve long-term marsh resilience alone, as it cannot address underlying causes of open water conversion. As a part of holistic climate planning that includes other management interventions, runnels may “buy time” for salt marshes to respond to management action, or adapt to sea level rise.
The Effects of ATIR Blocker on the Severity of COVID-19 in Hypertensive Inpatients and Virulence of SARS-CoV-2 in Hypertensive hACE2 Transgenic Mice
Authors: Jiang Xiaoliang , Li Huadong , Liu Yong , Bao Linlin , Zhan Lingjun , Gao Hong , Deng Wei , Xue Jing , Liu Jiangning , Liu Xing , Li Junli , Wang Jie , Wu Shuang , Yan Mingzhe , Luo Wei , Jose Pedro A. , Qin Chuan , Yang Xiuhong , Zhang Dingyu , Yang Zhiwei
Published: January 2022

Angiotensin-converting enzyme 2 (ACE2) is required for the cellular entry of the severe acute respiratory syndrome coronavirus 2. ACE2, via the Ang-(1-7)-Mas-R axis, is part of the antihypertensive and cardioprotective effects of the renin-angiotensin system. We studied hospitalized COVID-19 patients with hypertension and hypertensive human(h) ACE2 transgenic mice to determine the outcome of COVID-19 with or without AT1 receptor (AT1R) blocker treatment. The severity of the illness and the levels of serum cardiac biomarkers (CK, CK-BM, cTnI), as well as the inflammation markers (IL-1, IL-6, CRP), were lesser in hypertensive COVID-19 patients treated with AT1R blockers than those treated with other antihypertensive drugs. Hypertensive hACE2 transgenic mice, pretreated with AT1R blocker, had increased ACE2 expression and SARS-CoV-2 in the kidney and heart, 1 day post-infection. We conclude that those hypertensive patients treated with AT1R blocker may be at higher risk for SARS-CoV-2 infection. However, AT1R blockers had no effect on the severity of the illness but instead may have protected COVID-19 patients from heart injury, via the ACE2-angiotensin1-7-Mas receptor axis.
Critical stages for post-harvest losses and nutrition outcomes in the value chains of bush beans and nightshade in Uganda
Authors: Strecker Klara , Bitzer Verena , Kruijssen Froukje
Published: January 2022

The reduction of post-harvest losses (PHLs) has been identified as a key pathway to food and nutrition security in sub-Saharan Africa. However, despite policy prioritisation, knowledge about the severity of PHLs remains scant, especially when it comes to nutrient-dense crops such as African nightshade and bush beans. Therefore, this paper identifies loss hotspots, causes and effects throughout the value chains of nightshade and bush beans in eastern Uganda. Primary data collected following the Informal Food Loss Assessment Method, combined with small-scale load tracking and secondary data, allows for an analysis of physical, economic, quality, and nutritional losses throughout the value chains of both crops. Results show that in the bush bean value chain, severe physical and quality losses occur during post-harvest handling by farmers, leading to high economic losses at this stage of the chain. Nutritional losses are not expected to be significant in the bush bean value chain. By contrast, due to the shortness of the nightshade value chain, where produce is moved from harvest to consumption within one or two days, physical losses in most parts of the chain are relatively minor. Only at consumption stage, high physical losses occur. This is also the stage where economic losses and potential nutritional losses are most pronounced. The results of this study offer a deeper understanding of the value chain dynamics of bush beans and nightshade, including underlying gender relations, and identify concrete loss hotspots, upon which further research and practical interventions can build.
WaveRange: wavelet-based data compression for three-dimensional numerical simulations on regular grids
Authors: Kolomenskiy Dmitry , Onishi Ryo , Uehara Hitoshi
Published: January 2022

A wavelet-based method for compression of three-dimensional simulation data is presented and its software framework is described. It uses wavelet decomposition and subsequent range coding with quantization suitable for floating-point data. The effectiveness of this method is demonstrated by applying it to example numerical tests, ranging from idealized configurations to realistic global-scale simulations. The novelty of this study is in its focus on assessing the impact of compression on post-processing and restart of numerical simulations.

Graphical abstract
A moving liver phantom in an anthropomorphic thorax for SPECT MP imaging
Authors: Panagi S. , Hadjiconstanti Α. , Charitou G. , Kaolis D. , Petrou I. , Kyriacou C. , Parpottas Y.
Published: January 2022

Cranio-caudal respiratory motion and liver activity cause a variety of complex myocardial perfusion (MP) artifacts, especially in the inferior myocardial wall, that may also mask cardiac defects. To assess and characterise such artifacts, an anthropomorphic thorax with moving thoracic phantoms can be utilised in SPECT MP imaging. In this study, a liver phantom was developed and anatomically added into an anthropomorphic phantom that also encloses an ECG beating cardiac phantom and breathing lungs’ phantom. A cranio-caudal respiratory motion was also developed for the liver phantom and it was synchronised with the corresponding ones of the other thoracic phantoms. This continuous motion was further divided into isochronous dynamic respiratory phases, from end-exhalation to end-inspiration, to perform SPECT acquisitions in different respiratory phases. The new motions’ parameters and settings were measured by mechanical means and also validated in a clinical environment by acquiring CT images and by using two imaging software packages. To demonstrate the new imaging capabilities of the phantom assembly, SPECT/CT MP acquisitions were performed and compared to previous phantom and patients studies. All thoracic phantoms can precisely perform physiological motions within the anthropomorphic thorax. The new capabilities of the phantom assembly allow to perform SPECT/CT MP acquisitions for different cardiac-liver activity ratios and cardiac-liver proximities in supine and, for first time, in prone position. Thus, MP artifacts can be characterised and motion correction can be performed due to these new capabilities. The impact of artifacts and motion correction on defect detection can be also investigated.
Long-lasting rescue of schizophrenia-relevant cognitive impairments via risperidone-loaded microPlates
Authors: Bellotti Elena , Contarini Gabriella , Geraci Federica , Torrisi Sebastiano Alfio , Piazza Cateno , Drago Filippo , Leggio Gian Marco , Papaleo Francesco , Decuzzi Paolo
Published: January 2022

Schizophrenia is a disorder characterized by cognitive impairment and psychotic symptoms that fluctuate over time and can only be mitigated with the chronic administration of antipsychotics. Here, we propose biodegradable microPlates made of PLGA for the sustained release of risperidone over several weeks. Two microPlate configurations – short: 20 × 20 × 10 μm; tall: 20 × 20 × 20 μm – are engineered and compared to conventional ~ 10 μm PLGA microspheres in terms of risperidone loading and release. Tall microPlates realize the slowest release documenting a 35% risperidone delivery at 100 days with a residual rate of 30 ng/ml. Short microPlates and microspheres present similar release profiles with over 50% of the loaded risperidone delivered within the first 40 days. Then, the therapeutic efficacy of one single intraperitoneal injection of risperidone microPlates is compared to the daily administration of free risperidone in heterozygous knockout mice for dysbindin-1, a clinically relevant mouse model of cognitive and psychiatric liability. In temporal order object recognition tasks, mice treated with risperidone microPlates outperform those receiving free risperidone up to 2, 4, 8, and 12 weeks of observation. This suggests that the sustained release of antipsychotics from one-time microPlate deposition can rescue cognitive impairment in dysbindin mice for up to several weeks. Overall, these results demonstrate that risperidone-loaded microPlates are a promising platform for improving cognitive symptoms associated to schizophrenia. Moreover, the long-term efficacy with one single administration could be of clinical relevance in terms of patient’s compliance and adherence to the treatment regimen.

Graphical abstract

Single injection of long-acting risperidone-loaded µPL ameliorates the dysbindin-induced deficit in a clinically relevant mouse model of cognitive and psychiatric liability for up to 12 weeks
Factors affecting clinical manifestation of chromosomal imbalance in carriers of segmental autosomal mosaicism: differential impact of gender
Authors: Kovaleva Natalia V. , Cotter Philip D.
Published: January 2022

Mosaicism for unbalanced chromosomal rearrangements segmental mosaicism (SM) is rare, both in patients referred for cytogenetic testing and in prenatal diagnoses. In contrast, in preimplantation embryos SM is a frequent finding and, therefore, is even more challenging. However, there is no consistency among results of published studies on the clinical outcomes of embryos with SM, primarily due to the small number of reported cases. Moreover, there is the problem of predicting the potential for the optimal development of a mosaic embryo to a healthy individual. Therefore, we suggested comparing factors predisposing to favorable and poor prognoses, identified in postnatal and prenatal cohorts of SM carriers, with those obtained from studies on preimplantation embryos. We analyzed 580 published cases of SM including (i) postnatally diagnosed affected carriers, (ii) clinically asymptomatic carriers, (iii) prenatally diagnosed carriers, and (iv) miscarriages. We observed a concordance with preimplantation diagnoses regarding the clinical significance of the extent of mosaicism as well as a predominance of deletions over other types of rearrangements. However, there is no concordance regarding excessive involvement of chromosomes 1, 5, and 9 in unbalanced rearrangements and a preferential involvement of larger chromosomes compared to short ones. Paternal age was not found to be associated with SM in postnatally disease-defined individuals. We have identified maternal age and preferential involvement of chromosome 18 in rearrangements associated with clinical manifestations. Male predominance was found among normal pregnancy outcomes and among disease-defined carriers of rearrangements resulting in a gain of genomic material. Female predominance was found among abnormal pregnancy outcomes, among disease-defined carriers of loss and gain/loss rearrangements, and among transmitting carriers of gonadal SM, both affected and asymptomatic. According to data obtained from “post-embryo” studies, clinical manifestations of chromosomal imbalance are associated with a high proportion of abnormal cells, female gender, the type of rearrangement and involved chromosome(s), and maternal age. We believe these data are instructive in the challenging medical genetic counseling of parents faced with no option other than transfer of an embryo with segmental mosaicism.
Respiratory and cardiovascular effects of ambient particulate matter from dust storm and non-dust storm periods in Kuwait
Authors: Walters D. M. , Al-Khulaifi N. M. , Rushing B. R. , Selim M. I.
Published: January 2022

Epidemiological studies demonstrate a positive association between daily changes in concentrations of ambient airborne particulate matter (PM) and adverse respiratory and cardiovascular health effects. However, physicochemical properties of PM can vary greatly across geographical, atmospheric, and temporal conditions and influence the relative toxicity of airborne PM. The purpose of this study was to investigate the adverse pulmonary and cardiovascular health effects of ambient PM collected from discrete sampling sites in Kuwait during dust storm (DS) and non-dust storm (NDS) conditions. Collected dust samples were characterized for their chemical composition using atomic absorption, GC–MS, and HPLC–MS analyses. Male BALB/cJ mice were exposed to 100 µg of either NDS or dust storm (DS) PM in 50 µl of PBS by oropharyngeal aspiration. Lung function was measured and bronchoalveolar lavage was conducted at 1, 7, and 14 days post-exposure. Ischemia–reperfusion injury was performed 24 h after exposures by obstructing the left main coronary artery approximately 4 mm distal to its origin for 20 min, followed by 2 h. of reperfusion. Exposure to either NDS or DS PM resulted in airway hyperresponsiveness to acetylcholine compared to PBS controls. Total protein and cells in BAL fluid were elevated in both dust groups one day after exposure; however, DS PM induced a greater increase in cell numbers than did NDS PM, particularly in neutrophils, eosinophils, and lymphocytes. Representative lung sections exhibited positive staining for mucus in large airways at 7 days which resolved by 14 days in dust storm-exposed mice but persisted in NDS-exposed animals. Our findings suggest that NDS PM may be more effective in producing an adaptive immune response, while the early inflammation induced by DS PM may better resolve. We also observed a prolonged airway mucus response after exposure to NDS PM, suggesting it may produce more asthma-like features than dust storm PM. PM-induced changes to cardiac ischemia–reperfusion injury were not observed in this study. The lack of cardiovascular response may have been due to the limited exposure and single time point used in this study.
A comparative study of small-scale fishery supply chains’ vulnerability and resilience to COVID-19
Authors: Bassett Hannah R. , Sharan Sonia , Suri Sharon K. , Advani Sahir , Giordano Christopher
Published: January 2022

The COVID-19 pandemic and response has significantly disrupted fishery supply chains, creating shortages of essential foods and constraining livelihoods globally. Small-scale fisheries (SSFs) are responding to the pandemic in a variety of ways. Together, disruptions from and responses to COVID-19 illuminate existing vulnerabilities in the fish distribution paradigm and possible means of reducing system and actor sensitivity and exposure and increasing adaptive capacity. Integrating concepts from literature on supply chain disruptions, social-ecological systems, human wellbeing, vulnerability, and SSFs, we synthesize preliminary lessons from six case studies from Indonesia, the Philippines, Peru, Canada, and the United States. The SSF supply chains examined employ different distribution strategies and operate in different geographic, political, social, economic, and cultural contexts. Specifically, we ask (a) how resilient have different SSF supply chains been to COVID-19 impacts; (b) what do these initial outcomes indicate about the role of distribution strategies in determining the vulnerability of SSF supply chains to macroeconomic shocks; and (c) what key factors have shaped this vulnerability? Based on our findings, systemic changes that may reduce SSF vulnerability to future macroeconomic shocks include: diversification of distribution strategies, livelihoods, and products; development of local and domestic markets and distribution channels; reduced reliance on international markets; establishment of effective communication channels; and preparation for providing aid to directly assist supply chains and support consumer purchasing power.
A Survey on Some Recent Developments of Alternating Direction Method of Multipliers
Authors: Han De-Ren
Published: January 2022

Recently, alternating direction method of multipliers (ADMM) attracts much attentions from various fields and there are many variant versions tailored for different models. Moreover, its theoretical studies such as rate of convergence and extensions to nonconvex problems also achieve much progress. In this paper, we give a survey on some recent developments of ADMM and its variants.
Prospective long-term evaluation of incomplete distal renal tubular acidosis in idiopathic calcium nephrolithiasis diagnosed by low-dose NH4CL loading – gender prevalences and impact of alkali treatment
Authors: Sromicki Juri , Kacl Georg , Föhl Malin , Hess Bernhard
Published: January 2022

Purpose

Prospective evaluation of the prevalence of incomplete distal renal tubular acidosis (idRTA) in idiopathic calcium stone formers (ICSF) diagnosed by half-dose ammonium chloride loading (NH4Cl, 0.05 g/kg body weight/day) and impact of alkali treatment of idRTA.

Methods

Evaluation of 386 consecutive idiopathic calcium stone formers (ICSF) (280 males, 106 females) for idRTA. If screening fasting urine pH was > 5.80, 1-day NH4Cl loading was performed without severe adverse effects. Normally, urine pH falls below 5.45.

Results

Sixty-four idiopathic calcium stone formers exhibited idRTA, one complete dRTA. Prevalence was higher in women (25.4%) than in men (13.6%). Thus, for more equilibrated comparisons, we formed pairs of 62 idiopathic calcium stone formers (ICSF) with and 62 without idRTA, matched for gender, age, BMI and serum creatinine. Idiopathic calcium stone formers with idRTA more often had hypercalciuria (p < 0.025) and urine citrate < 2 mmol/d (p < 0.05), formed calcium phosphate stones more frequently, exhibited higher numbers of stones/year (1.4 ± 1.5 vs. 0.9 ± 0.8, p = 0.034) and 2.5 times more intrarenal calcifications (4.6 ± 5.9 vs. 1.8 ± 3.6, p = 0.002). All idiopathic calcium stone formers with idRTA were recommended chronic alkali therapy. After 4–15 years of follow-up, stone events /years follow-up (stone passage or urologic intervention) were higher in patients non-adherent to alkali therapy (0.61 ± 0.92) than in patients adherent to treatment (0.11 ± 0.21, p = 0.006).

Conclusion

Incomplete distal renal tubular acidosis is 1.8-fold more prevalent among female idiopathic calcium stone formers, predicts more stone recurrences, predisposes to calcium phosphate stones and is associated with 2.5 times more intrarenal calcifications vs. non-idRTA patients. Chronic alkali treatment reduces clinical stone recurrences by 5.5 times.

Graphical abstract
Assessment of a Geothermal Combined System with an Organic Rankine Cycle and Multi-effect Distillation Desalination
Authors: Farsi Aida , Rosen Marc A.
Published: January 2022

An analysis is reported of a geothermal-based electricity-freshwater system in which an organic Rankine cycle is integrated with a multi-effect distillation desalination unit. The system is driven by geothermal hot water extracted from the production well. Mass, energy, entropy, and exergy rate balances are written for all system components, as are energy and exergy efficiency expressions for each subsystem. The exergy destruction rate associated with the temperature and chemical disequilibrium of the freshwater and brine with the reference environment are taken into account to reveal accurate results for irreversibility sources within the desalination process. The developed thermodynamic model is simulated using thermodynamic properties of the working fluids (i.e., ammonia, seawater, distillate, and brine) at each state point. A sustainability analysis is performed that connects exergy and environmental impact concepts. That assessment expresses the extent of the contribution of the system to sustainable development and reduced environmental impact, using exergy methods. Results of the sustainability analysis indicate that, with an increase in the reference environment temperature from 20 to 35 $$^\circ{\rm C}$$

∘

C

, the exergy destruction rate decreases for the multi-effect distillation and organic Rankine cycle systems respectively from 6474 to 4217 kW and from 16,270 to 13,459 kW. Also, the corresponding sustainability index for the multi-effect distillation and organic Rankine cycle systems increases from 1.16 to 1.2 and 1.5–1.6, respectively, for the same increase in reference environment temperature.
Hydrodynamic Modelling of Floods and Estimating Socio-economic Impacts of Floods in Ugandan River Malaba Sub-catchment
Authors: Mubialiwo Ambrose , Abebe Adane , Kawo Nafyad Serre , Ekolu Job , Nadarajah Saralees , Onyutha Charles
Published: January 2022

River Malaba sub-catchment tends to experience dramatic flooding events, with several socio-economic impacts to the nearby communities, such as loss of lives and destructions of physical infrastructure. Analysis of spatiotemporal extents to which settlements, crops and physical infrastructures tend to be inundated are vital for predictive planning of risk-based adaptation measures. This paper presents a case study on flood risk assessment for Ugandan River Malaba sub-catchment. We applied the two-dimensional Hydraulic Engineering Center’s River Analysis System (2D HEC-RAS) for modelling of flooding extents. We considered extreme flow quantiles, lower and upper quantiles corresponding to the 95% confidence interval limits aimed at determining uncertainties in the flooding extents. Spatial extents of inundation on human settlement, land cover and infrastructure were analysed with respect to return periods of extreme flow quantiles. Finally, we estimated economic loss on infrastructure due to flooding. Results from the 2D HEC-RAS model were satisfactorily comparable with the results of observations. Amongst the land use types, cropland exhibited the highest vulnerability with at least 10,234.8 hectare (ha) susceptible to flooding event of 100-year return period (YRP). Inundated built-up land-use exhibited the highest vulnerability percentage increase (90%) between 2- and 100-YRP. In US Dollar, about US$ 33 million and US$ 39 million losses are estimated at 2- and 100-YRP, respectively, due to inundated rice gardens and these indicate a looming high risk of household food insecurity and poverty. Several infrastructure including 15 academic institutions, 12 health facilities, 32 worshiping places remain annually vulnerable to flooding. At least 6 km and 7 km of road network are also susceptible to flooding under extreme flows of return periods 2 and 100 years, respectively. Churches exhibited the highest economic losses of US$ 855,065 and US$ 1,623,832 at 2-YRP and 100-YRP, respectively. This study findings are relevant for planning the development of sustainable flood risk adaptation pathways given the established destructions within the sub-catchment due to flooding.
Marked point processes and intensity ratios for limit order book modeling
Authors: Muni Toke Ioane , Yoshida Nakahiro
Published: January 2022

This paper extends the analysis of Muni Toke and Yoshida (2020) to the case of marked point processes. We consider multiple marked point processes with intensities defined by three multiplicative components, namely a common baseline intensity, a state-dependent component specific to each process, and a state-dependent component specific to each mark within each process. We show that for specific mark distributions, this model is a combination of the ratio models defined in Muni Toke and Yoshida (2020). We prove convergence results for the quasi-maximum and quasi-Bayesian likelihood estimators of this model and provide numerical illustrations of the asymptotic variances. We use these ratio processes to model transactions occurring in a limit order book. Model flexibility allows us to investigate both state-dependency (emphasizing the role of imbalance and spread as significant signals) and clustering. Calibration, model selection and prediction results are reported for high-frequency trading data on multiple stocks traded on Euronext Paris. We show that the marked ratio model outperforms other intensity-based methods (such as “pure” Hawkes-based methods) in predicting the sign and aggressiveness of market orders on financial markets.
Significant influence of self-possessed moisture of limonitic nickel laterite on sintering performance and its action mechanism
Authors: Xue Yu-xiao , Zhu De-qing , Pan Jian , Guo Zheng-qi , Tian Hong-yu , Li Gen , Huang Qing-zhou , Pan Liao-ting , Huang Xue-zhong
Published: January 2022

In consideration of the abundant moisture of limonitic nickel laterite mined, it is essential to determine whether the self-possessed moisture of limonitic nickel laterite after pre-dried is appropriate for sintering. Thus, based on the characterization of limonitic nickel laterite, the influence of its self-possessed moisture on sintering performance was expounded by sinter pot tests and the relevant mechanism was revealed by the systematical analyses of the granulation properties of sinter mixture, thermodynamic conditions during sintering and mineralogy of product sinter. The results indicate that the self-possessed moisture of limonitic nickel laterite indeed has significant influence on its sintering performance. At the optimum self-possessed moisture of 21 mass%, sinter indices are relatively better with tumble index, productivity and solid fuel rate of 48.87%, 1.04 t m−2 h−1 and 136.52 kg t−1, respectively, due to the superior granulation properties of sinter mixture and thermodynamic conditions during sintering, relatively large amount of silico-ferrite of calcium and alumina and tighter sinter microstructure. However, sintering performance of limonitic nickel laterite is still much poorer than that of ordinary iron ores. It is feasible to strengthen limonitic nickel laterite sintering by inhibiting the over-fast sintering speed and improving the thermodynamic conditions during sintering.
Star Tracker Geometric Calibration Through Full-State Estimation Including Attitude
Authors: Yoon Hyosang , Baeck Kiwook , Wi Junsung
Published: January 2022

A star tracker calibration method using star images is presented in this paper. Unlike previous works, the proposed method estimates all parameters and the attitudes at once in a single least-squares formulation for the optimal calibration, which can be easily converted to a recursive estimation form. In addition, this paper presents a method to estimate the overall star tracker performance for attitude determination from the calibration results. Since the proposed method uses star images only, it can be applied to both on-orbit and ground star tracker calibration. The simulations show improvements in calibration performance about four times compared to the previous calibration method. The calibration experiments with actual star images are conducted to test its application.
Selenium Decreases the Cadmium Content in Brown Rice: Foliar Se Application to Plants Grown in Cd-contaminated Soil
Authors: Jiang Shuochen , Du Bin , Wu Qixia , Zhang Haiwei , Deng Youyu , Tang Xiangru , Zhu Jianqiang
Published: January 2022

Cadmium (Cd) contamination in agricultural soils has become a serious issue owing to its high toxicity threat to human health through the food chain. The purpose of this paper is to explore the availability of foliar selenium (Se) application in reducing Cd enrichment in brown rice. A field experiment from 2017 to 2019 was conducted to investigate the effects of foliar Se application on the physiology and yields of three rice cultivars and their accumulation of Cd in low-Cd and high-Cd soils. The grain protein contents and yields of rice plants grown in the high-Cd soil were lower than those of plants cultivated in the low-Cd soil by 27.85% and 6.82%, whereas the malondialdehyde (MDA) and Cd contents were higher by 66.06% and 91.47%, respectively. Se application reduced Cd translocation from the stems and leaves to the spikes, decreasing the Cd content in brown rice by 40.36%. Additionally, Se enhanced the antioxidative activity, glutathione and protein contents, and rice yield (7.58%) and decreased the MDA and proline contents. However, these Se effects weakened under the high-Cd soil. Foliar Se application can alleviate Cd-induced physiological stress in brown rice while improving its yield and reducing its Cd content.
Metabolic tuning of a stable microbial community in the surface oligotrophic Indian Ocean revealed by integrated meta-omics
Authors: Xie Zhang-Xian , Yan Ke-Qiang , Kong Ling-Fen , Gai Ying-Bao , Jin Tao , He Yan-Bin , Wang Ya-Yu , Chen Feng , Lin Lin , Lin Zhi-Long , Xu Hong-Kai , Shao Zong-Ze , Liu Si-Qi , Wang Da-Zhi
Published: January 2022

Understanding the mechanisms, structuring microbial communities in oligotrophic ocean surface waters remains a major ecological endeavor. Functional redundancy and metabolic tuning are two mechanisms that have been proposed to shape microbial response to environmental forcing. However, little is known about their roles in the oligotrophic surface ocean due to less integrative characterization of community taxonomy and function. Here, we applied an integrated meta-omics-based approach, from genes to proteins, to investigate the microbial community of the oligotrophic northern Indian Ocean. Insignificant spatial variabilities of both genomic and proteomic compositions indicated a stable microbial community that was dominated by Prochlorococcus, Synechococcus, and SAR11. However, fine tuning of some metabolic functions that are mainly driven by salinity and temperature was observed. Intriguingly, a tuning divergence occurred between metabolic potential and activity in response to different environmental perturbations. Our results indicate that metabolic tuning is an important mechanism for sustaining the stability of microbial communities in oligotrophic oceans. In addition, integrated meta-omics provides a powerful tool to comprehensively understand microbial behavior and function in the ocean.
Contrasting patterns of bacterial communities in the rearing water and gut of Penaeus vannamei in response to exogenous glucose addition
Authors: Huang Lei , Guo Haipeng , Liu Zidan , Chen Chen , Wang Kai , Huang Xiaolin , Chen Wei , Zhu Yueyue , Yan Mengchen , Zhang Demin
Published: January 2022

Supplementing exogenous carbon sources is a practical approach to improving shrimp health by manipulating the microbial communities of aquaculture systems. However, little is known about the microbiological processes and mechanisms of these systems. Here, the effects of glucose addition on shrimp growth performance and bacterial communities of the rearing water and the shrimp gut were investigated to address this knowledge gap. The results showed that glucose addition significantly improved the growth and survival of shrimp. Although the α-diversity indices of both bacterioplankton communities and gut microbiota were significantly decreased by adding glucose, both bacterial communities exhibited divergent response patterns to glucose addition. Glucose addition induced a dispersive bacterioplankton community but a more stable gut bacterial community. Bacterial taxa belonging to Ruegeria were significantly enriched by glucose in the guts, especially the operational taxonomic unit 2575 (OTU2575), which showed the highest relative importance to the survival rate and individual weight of shrimp, with the values of 43.8 and 40.6%, respectively. In addition, glucose addition increased the complexity of interspecies interactions within gut bacterial communities and the network nodes from Rhodobacteraceae accounted for higher proportions and linked more with the nodes from other taxa in the glucose addition group than that in control. These findings suggest that glucose addition may provide a more stable gut microbiota for shrimp by increasing the abundance of certain bacterial taxa, such as Ruegeria.
Prevalence of common aneuploidy in twin pregnancies
Authors: Konishi Akiko , Samura Osamu , Muromoto Jin , Okamoto Yoko , Takahashi Hironori , Kasai Yasuyo , Ichikawa Mayuko , Yamada Naoki , Kato Noriko , Sato Hiroshi , Hamada Hiromi , Nakanami Naoyuki , Machi Maya , Ichizuka Kiyotake , Sunami Rei , Tanaka Toshitaka , Yonetani Naoto , Kamei Yoshimasa , Nagamatsu Takeshi , Matsumoto Mariko , Tairaku Shinya , Fujiwara Arisa , Nakamura Hiroaki , Harada Takashi , Watanabe Takafumi , Sasaki Shoko , Kawaguchi Satoshi , Minami Sawako , Ogawa Masaki , Miura Kiyonori , Suzumori Nobuhiro , Kojima Junya , Kotani Tomomi , Sasaki Rumi , Baba Tsukasa , Toyofuku Aya , Endo Masayuki , Takeshita Naoki , Taketani Takeshi , Sase Masakatsu , Matsubara Keiichi , Hayata Kei , Hamada Yoshinobu , Egawa Makiko , Kakinuma Toshiyuki , Matsushima Sachio , Kitagawa Michihiro , Shiga Tomomi , Kurashina Ryuhei , Hamada Hironori , Takagi Hiroaki , Kondo Akane , Miharu Norio , Yamashita Michiko , Horiya Madoka , Morimoto Keiji , Takahashi Ken , Okamoto Aikou , Sekizawa Akihiko , Sago Haruhiko
Published: January 2022

The incidence of chromosomal abnormalities in twin pregnancies is not well-studied. In this retrospective study, we investigated the frequency of chromosomal abnormalities in twin pregnancies and compared the incidence of chromosomal abnormalities in dichorionic diamniotic (DD) and monochorionic diamniotic (MD) twins. We used data from 57 clinical facilities across Japan. Twin pregnancies of more than 12 weeks of gestation managed between January 2016 and December 2018 were included in the study. A total of 2899 and 1908 cases of DD and MD twins, respectively, were reported, and the incidence of chromosomal abnormalities in one or both fetuses was 0.9% (25/2899) and 0.2% (4/1908) in each group (p = 0.004). In this study, the most common chromosomal abnormality was trisomy 21 (51.7% [15/29]), followed by trisomy 18 (13.8% [4/29]) and trisomy 13 (6.9% [2/29]). The incidence of trisomy 21 in MD twins was lower than that in DD twins (0.05% vs. 0.5%,p = 0.007). Trisomy 21 was less common in MD twins, even when compared with the expected incidence in singletons (0.05% vs. 0.3%, RR 0.15 [95% CI 0.04–0.68]). The risk of chromosomal abnormality decreases in twin pregnancies, especially in MD twins.
The utility of the Edmonton Obesity Staging System for the prediction of COVID-19 outcomes: a multi-centre study
Authors: Rodríguez-Flores Marcela , Goicochea-Turcott Eduardo W. , Mancillas-Adame Leonardo , Garibay-Nieto Nayely , López-Cervantes Malaquías , Rojas-Russell Mario E. , Castro-Porras Lilia V. , Gutiérrez-León Eduardo , Campos-Calderón Luis F. , Pedraza-Escudero Karen , Aguilar-Cuarto Karina , Villanueva-Ortega Eréndira , Hernández-Ruíz Joselin , Guerrero-Avendaño Guadalupe , Monzalvo-Reyes Sheyla M. , García-Rascón Rafael , Gil-Velázquez Israel N. , Cortés-Hernández Dora E. , Granados-Shiroma Marcela , Alvarez-Rodríguez Brenda G. , Cabello-Garza Martha L. , González-Contreras Zaira L. , Picazo-Palencia Esteban , Cerda-Arteaga Juana M. , Pérez-Gómez Héctor R. , Calva-Rodríguez Roberto , Sánchez-Rodríguez Gerardo , Carpio-Vázquez Leslie D. , Dávalos-Herrera María A. , Villatoro-de-Pleitez Karla M. , Suárez-López Melissa D. , Nevárez-Carrillo María G. , Pérez-Alcántara Karina , Mehta Roopa , Diez Edurne Sandoval , Gregg Edward W.
Published: January 2022

Background

Patients with obesity have an increased risk for adverse COVID-19 outcomes. Body mass index (BMI) does not acknowledge the health burden associated this disease. The performance of the Edmonton Obesity Staging System (EOSS), a clinical classification tool that assesses obesity-related comorbidity, is compared with BMI, with respect to adverse COVID-19 outcomes.

Methods

1071 patients were evaluated in 11 COVID-19 hospitals in Mexico. Patients were classified into EOSS stages. Adjusted risk factors for COVID-19 outcomes were calculated and survival analysis for mechanical ventilation and death was carried out according to EOSS stage and BMI category.

Results

The risk for intubation was higher in patients with EOSS stages 2 and 4 (HR 1.42, 95% CI 1.02–1.97 and 2.78, 95% CI 1.83–4.24), and in patients with BMI classes II and III (HR 1.71, 95% CI 1.06–2.74, and 2.62, 95% CI 1.65–4.17). Mortality rates were significantly lower in patients with EOSS stages 0 and 1 (HR 0.62, 95% CI 0.42–0.92) and higher in patients with BMI class III (HR 1.58, 95% CI 1.03–2.42). In patients with a BMI ≥ 25 kg/m2, the risk for intubation increased with progressive EOSS stages. Only individuals in BMI class III showed an increased risk for intubation (HR 2.24, 95% CI 1.50–3.34). Mortality risk was increased in EOSS stages 2 and 4 compared to EOSS 0 and 1, and in patients with BMI class II and III, compared to patients with overweight.

Conclusions

EOSS was associated with adverse COVID-19 outcomes, and it distinguished risks beyond BMI. Patients with overweight and obesity in EOSS stages 0 and 1 had a lower risk than patients with normal weight. BMI does not adequately reflect adipose tissue-associated disease, it is not ideal for guiding chronic-disease management.
Creating a small baby program: a single center’s experience
Authors: Banerji Anamika I. , Hopper Andrew , Kadri Munaf , Harding Benjamin , Phillips Raylene
Published: January 2022

Creation of a small baby program requires special resources and multidisciplinary engagement. Such a program has the potential to improve patient care, parent and staff satisfaction, collaboration and communication. We have described benefits, challenges, and practical approaches to creating and maintaining a small baby program that could be a model for the development of special programs for other sub-populations within in the NICU.
A thalamo-centric neural signature for restructuring negative self-beliefs
Authors: Steward Trevor , Kung Po-Han , Davey Christopher G. , Moffat Bradford A. , Glarin Rebecca K. , Jamieson Alec J. , Felmingham Kim L. , Harrison Ben J.
Published: January 2022

Negative self-beliefs are a core feature of psychopathology. Despite this, we have a limited understanding of the brain mechanisms by which negative self-beliefs are cognitively restructured. Using a novel paradigm, we had participants use Socratic questioning techniques to restructure negative beliefs during ultra-high resolution 7-Tesla functional magnetic resonance imaging (UHF 7 T fMRI) scanning. Cognitive restructuring elicited prominent activation in a fronto-striato-thalamic circuit, including the mediodorsal thalamus (MD), a group of deep subcortical nuclei believed to synchronize and integrate prefrontal cortex activity, but which has seldom been directly examined with fMRI due to its small size. Increased activity was also identified in the medial prefrontal cortex (MPFC), a region consistently activated by internally focused mental processing, as well as in lateral prefrontal regions associated with regulating emotional reactivity. Using Dynamic Causal Modelling (DCM), evidence was found to support the MD as having a strong excitatory effect on the activity of regions within the broader network mediating cognitive restructuring. Moreover, the degree to which participants modulated MPFC-to-MD effective connectivity during cognitive restructuring predicted their individual tendency to engage in repetitive negative thinking. Our findings represent a major shift from a cortico-centric framework of cognition and provide important mechanistic insights into how the MD facilitates key processes in cognitive interventions for common psychiatric disorders. In addition to relaying integrative information across basal ganglia and the cortex, we propose a multifaceted role for the MD whose broad excitatory pathways act to increase synchrony between cortical regions to sustain complex mental representations, including the self.
The default network is causally linked to creative thinking
Authors: Shofty Ben , Gonen Tal , Bergmann Eyal , Mayseless Naama , Korn Akiva , Shamay-Tsoory Simone , Grossman Rachel , Jalon Itamar , Kahn Itamar , Ram Zvi
Published: January 2022

Creative thinking represents a major evolutionary mechanism that greatly contributed to the rapid advancement of the human species. The ability to produce novel and useful ideas, or original thinking, is thought to correlate well with unexpected, synchronous activation of several large-scale, dispersed cortical networks, such as the default network (DN). Despite a vast amount of correlative evidence, a causal link between default network and creativity has yet to be demonstrated. Surgeries for resection of brain tumors that lie in proximity to speech related areas are performed while the patient is awake to map the exposed cortical surface for language functions. Such operations provide a unique opportunity to explore human behavior while disrupting a focal cortical area via focal electrical stimulation. We used a novel paradigm of individualized direct cortical stimulation to examine the association between creative thinking and the DN. Preoperative resting-state fMRI was used to map the DN in individual patients. A cortical area identified as a DN node (study) or outside the DN (controls) was stimulated while the participants performed an alternate-uses-task (AUT). This task measures divergent thinking through the number and originality of different uses provided for an everyday object. Baseline AUT performance in the operating room was positively correlated with DN integrity. Direct cortical stimulation at the DN node resulted in decreased ability to produce alternate uses, but not in the originality of uses produced. Stimulation in areas that when used as network seed regions produced a network similar to the canonical DN was associated with reduction of creative fluency. Stimulation of areas that did not produce a default-like network (controls) did not alter creative thinking. This is the first study to causally link the DN and creative thinking.
CD147 supports paclitaxel resistance via interacting with RanBP1
Authors: Nan Gang , Zhao Shu-Hua , Wang Ting , Chao Dong , Tian Ruo-Fei , Wang Wen-Jing , Fu Xin , Lin Peng , Guo Ting , Wang Bin , Sun Xiu-Xuan , Chen Xi , Chen Zhi-Nan , Wang Shi-Jie , Cui Hong-Yong
Published: January 2022

Though the great success of paclitaxel, the variable response of patients to the drug limits its clinical utility and the precise mechanisms underlying the variable response to paclitaxel remain largely unknown. This study aims to verify the role and the underlying mechanisms of CD147 in paclitaxel resistance. Immunostaining was used to analyze human non-small-cell lung cancer (NSCLC) and ovarian cancer tissues. RNA-sequencing was used to identify downstream effectors. Annexin V-FITC/propidium iodide and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were used to detect apoptosis. Co-immunoprecipitation (Co-IP), fluorescence resonance energy transfer (FRET) and surface plasmon resonance (SPR) were performed to determine protein interactions. Fluorescence recovery after photobleaching (FRAP) was performed to measure the speed of microtubule turnover. Xenograft tumor model was established to evaluate sensitivity of cancer cells to paclitaxel in vivo. In vitro and in vivo assays showed that silencing CD147 sensitized the cancer cells to paclitaxel treatment. CD147 protected cancer cells from paclitaxel-induced caspase-3 mediated apoptosis regardless of p53 status. Truncation analysis showed that the intracellular domain of CD147 (CD147ICD) was indispensable for CD147-regulated sensitivity to paclitaxel. Via screening the interacting proteins of CD147ICD, Ran binding protein 1 (RanBP1) was identified to interact with CD147ICD via its C-terminal tail. Furthermore, we showed that RanBP1 mediated CD147-regulated microtubule stability and dynamics as well as response to paclitaxel treatment. These results demonstrated that CD147 regulated paclitaxel response by interacting with the C-terminal tail of RanBP1 and targeting CD147 may be a promising strategy for preventing paclitaxel resistant.
Human gut-microbiome-derived propionate coordinates proteasomal degradation via HECTD2 upregulation to target EHMT2 in colorectal cancer
Authors: Ryu Tae Young , Kim Kwangho , Han Tae-Su , Lee Mi-Ok , Lee Jinkwon , Choi Jinhyeon , Jung Kwang Bo , Jeong Eun-Jeong , An Da Mi , Jung Cho-Rok , Lim Jung Hwa , Jung Jaeeun , Park Kunhyang , Lee Moo-Seung , Kim Mi-Young , Oh Soo Jin , Hur Keun , Hamamoto Ryuji , Park Doo-Sang , Kim Dae-Soo , Son Mi-Young , Cho Hyun-Soo
Published: January 2022

The human microbiome plays an essential role in the human immune system, food digestion, and protection from harmful bacteria by colonizing the human intestine. Recently, although the human microbiome affects colorectal cancer (CRC) treatment, the mode of action between the microbiome and CRC remains unclear. This study showed that propionate suppressed CRC growth by promoting the proteasomal degradation of euchromatic histone-lysine N-methyltransferase 2 (EHMT2) through HECT domain E3 ubiquitin protein ligase 2 (HECTD2) upregulation. In addition, EHMT2 downregulation reduced the H3K9me2 level on the promoter region of tumor necrosis factor α-induced protein 1 (TNFAIP1) as a novel direct target of EHMT2. Subsequently, TNFAIP1 upregulation induced the apoptosis of CRC cells. Furthermore, using Bacteroides thetaiotaomicron culture medium, we confirmed EHMT2 downregulation via upregulation of HECTD2 and TNFAIP1 upregulation. Finally, we observed the synergistic effect of propionate and an EHMT2 inhibitor (BIX01294) in 3D spheroid culture models. Thus, we suggest the anticancer effects of propionate and EHMT2 as therapeutic targets for colon cancer treatment and may provide the possibility for the synergistic effects of an EHMT2 inhibitor and microbiome in CRC treatment.
O-GlcNAcylation of MORC2 at threonine 556 by OGT couples TGF-β signaling to breast cancer progression
Authors: Liu Ying-Ying , Liu Hong-Yi , Yu Tian-Jian , Lu Qin , Zhang Fang-Lin , Liu Guang-Yu , Shao Zhi-Ming , Li Da-Qiang
Published: January 2022

MORC family CW-type zinc finger 2 (MORC2) is a newly identified chromatin-remodeling enzyme involved in DNA damage response and gene transcription, and its dysregulation has been linked with Charcot-Marie-Tooth disease, neurodevelopmental disorder, and cancer. Despite its functional importance, how MORC2 is regulated remains enigmatic. Here, we report that MORC2 is O-GlcNAcylated by O-GlcNAc transferase (OGT) at threonine 556. Mutation of this site or pharmacological inhibition of OGT impairs MORC2-mediated breast cancer cell migration and invasion in vitro and lung colonization in vivo. Moreover, transforming growth factor-β1 (TGF-β1) induces MORC2 O-GlcNAcylation through enhancing the stability of glutamine-fructose-6-phosphate aminotransferase (GFAT), the rate-limiting enzyme for producing the sugar donor for OGT. O-GlcNAcylated MORC2 is required for transcriptional activation of TGF-β1 target genes connective tissue growth factor (CTGF) and snail family transcriptional repressor 1 (SNAIL). In support of these observations, knockdown of GFAT, SNAIL or CTGF compromises TGF-β1-induced, MORC2 O-GlcNAcylation-mediated breast cancer cell migration and invasion. Clinically, high expression of OGT, MORC2, SNAIL, and CTGF in breast tumors is associated with poor patient prognosis. Collectively, these findings uncover a previously unrecognized mechanistic role for MORC2 O-GlcNAcylation in breast cancer progression and provide evidence for targeting MORC2-dependent breast cancer through blocking its O-GlcNAcylation.