bluuebunny/crossref_metadata_2025 huggingface dataset:
Dakwah Dengan Seni Musik
Authors: Hanif Fuadi Muhamad , Alfarisi Salman
Published: July 2023

The purpose of this research is to reveal the phenomenon of da'wah with the art of music as a means to attract mad’u, especially young people. Da'wah and music are both mutually beneficial. Da'wah as an effort to call for goodness while the art of music makes beauty in calling for goodness. The progress of the times is a step to innovate in the development of da'wah, in music the lyrics as a means of propaganda, young people as targets of propaganda even for group personnel to become a means of propaganda in the field of economic empowerment. This study uses qualitative methods as an effort to reveal the meaning behind the establishment and activities from stage to stage of the Qasidah Group. Field observations, in-depth interviews and documentation became the material for analysis in this study. The results of this study reveal innovation in da'wah namely packaging messages of aqidah, Sharia and morals with music, the basis of religious education from Islamic boarding schools as a philosophy in concocting da'wah messages in music, enthusiastic young people who see appearances on stage as an internalization of Islamic values, then this group becomes a means for economic empowerment of its members so that they can support their families from every stage to stage.
ANALISIS SEGRAGASI GENOTIP BIOAKTIF DAN MORFOLOGI PADA TURUNAN F2 HASIL PERSILANGAN KETAN HITAM DAN CIHERANG
Authors: Nugroho Puspito Agung , Deviga Intan Fia , Ubaidillah Mohammad , Rhamadinda Nendra Tigara Mellfani
Published: July 2023

Tanaman padi dapat dikelompokkan menjadi 2 jenis, yaitu padi pigmented dan non pigmented. Salah satu contoh padi berpigmen yaitu padi beras merah dan padi beras hitam. Padi berpigmen dikenal sebagai sumber senyawa antioksidan termasuk flavonoid, antosianin, dan senyawa fenolik. Penelitian ini bertujuan untuk menganalisis segregasi hasil persilangan tanaman padi varietas Ketan Hitam dengan Ciherang melalui analisis karakter morfologi dan genotip bioaktif menggunakan marka molekuler SSR. Metode penelitian yang dilakukan yaitu dengan persiapan dan pemeliharaan tanaman (persiapan bahan tanam, pengolahan tanah, penanaman, pemupukan, pengairan, penyiangan, dan pemanenan) dan analisis genotip bioaktif (ekstraksi DNA, proses kerja PCR, elektroforesis dan pengamatan UV Transluminator). Analisis PCR menggunaan marka SSR dengan primer RM 346, RM 316, RM 228, dan RM 339. Analisis data yang digunakan adalah analisis distribusi frekuensi kuantitatif dengan panduan IRRI. Segragasi karakter morfologi pada populasi F2 hasil persilangan antara Ketan Hitam dan Ciherang masih tinggi, dimana dapat dilihat dari pola segragasi karakter pada tinggi tanaman, jumlah anakan, anakan produktif dan berat 1000 bijinya yang dikendalikan oleh banyak gen dan dipengaruhi oleh faktor lingkungan di dalamnya karena tidak memenuhi nisbah genetika mendel ataupun penyimpangannya. Bentuk morfologi hasil persilangannya lebih cenderung mirip dengan tetua betina atau Ketan Hitam. Sedangkan untuk hasil PCR dengan menggunakan marka SSR menunjukkan bahwa RM 346, RM 339 dan RM 228 dapat memvalidasi keseluruhan sampel yang digunakan baik tetua persilangan maupun F2 hasil persilangannya, sedangkan untuk RM 316 tidak dapat memvalidasi keseluruhan pita-pita DNA pada sampel penelitian.
PERSEPSI APLIKASI STANDART NURSING LANGUAGE DALAM DOKUMENTASI ASUHAN KEPERAWATAN
Authors: Dwi Fibriansari Rizeki , Astuti Anggia , Maisyaroh Arista , Prasetya Widianto Eko
Published: July 2023

Kualitas dokumentasi keperawatan sangat dipengaruhi oleh kepatuhan perawat terhadap aturan pendokumentasian yang ditetapkan oleh profesi maupun pemerintah. Pelaksanaan dokumentasi asuhan keperawatan digunakan sebagai alat ukur untuk menyadari dan mengamati kualitas pelayanan asuhan keperawatan yang dilaksanakan di rumah sakit. Penelitian ini bertujuan untuk mengindentifikasi persepsi perawat dalam aplikasi standar bahasa keperawatan 3S (SDKI, SLKI dan SIKI) pada dokumentasi keperawatan. Desain penelitian ini adalah penelitian deskriptif. Subyek penelitian ini adalah standar bahasa keperawatan dalam dokumentasi asuhan keperawatan. Analisis data dalam penelitian ini dilakukan pada tahap reduksi data, penyajian data, dan penarikan kesimpulan. Data divalidasi dengan triangulasi dan triangulasi metode. Hasil penelitian menunjukkan bahwa 52% perawat mendapatkan sumber informasi dari buku panduan dan hanya 42,6% perawat memahami aplikasi standar bahasa keperawatan 3S. Penyebab permasalahan ini adalah pencatatan tindakan yang kurang lengkap dan data pengkajian yang kurang kosisten dengan diagnosis keperawatan. Selain itu pencatatan tindakan keperawatan yang terlalu banyak sehingga banyak menghabiskan waktu perawat. Sehingga perlu dibuat standar bahasa keperawatan sesuai 3S yang efektif dan efisien. Pelatihan dokumentasi standar keperawatan Indonesia perlu dilakukan untuk menyamakan persepsi dan memberikan dampak yang baik terhadap pengetahuan perawat dan mutu pelayanan.
EKSTRAKSI PROTEIN DARI SPIRULINA SP DALAM PELARUT ETANOL TERBANTUKAN GELOMBANG ULTRASONIK
Authors: Fachri Boy Arief , Palupi Bekti , Rahmawati Istiqomah , Rizkiana Meta Fitri , Amini Helda Wika
Published: July 2023

Protein merupakan komponen nutrisi yang dibutuhkan oleh manusia untuk kesehatan tubuh. Salah satu sumber protein yang melimpah di Indonesia adalah alga Spirulina Sp. Ekstraksi protein alga, sulit dilakukan. Hal ini dikarenakan, protein tersimpan dalam sel-sel alga yang memiliki dinding yang sangat keras. Metode konvensional yang selama ini dilakukan membutuhkan waktu yang sangat lama, dan energi yang besar. Untuk mengatasi hal itu, sebuah metode alternatif yang diajukan dalam penelitian ini, adalah metode ekstraksi ultrasonik. Tujuan penelitian ini adalah untuk menentukan kondisi optimum ekstraksi protein dari Spirulina Sp dan mengetahui pengaruh parameter ekstraksi terhadap yield. Sprirulina Sp yang tekah dikeringkan dan dihaluskan diumpankan ke dalam ekstraktor yang berisi etanol. Gelombang ultrasonic, kemudian dialirkan ke dalam ekstraktor melalui probe. Setelah, eaktu ekstraksi tercapai, ekstrak dan residu dipisahkan dengan cara sentrifugasi dengan kecepatan 10000 rpm pada suhu kamar. Selanjutnya, yield protein ekstrak dihitung dengan analisa Lowry-Bradford. Pengaruh parameter proses seperti rasio bahan baku-pelarut (0:1-0.5) g/mL, ukuran partikel bahan baku (600-800) mesh dan waktu ekstraksi 15-45 menit) terhadap yield akan diamati dalam penelitian ini.  Berdasarkan hasil penelitian, dapat disimpulkan bahwa yield tertinggi sebesar 80,87 % diperoleh pada kondisi rasio bahan baku-pelarut 0.2 g/mL, ukuran partikel bahan baku 700 mesh dan waktu ekstraksi 30 menit. Sementara itu, ukuran partikel  merupakan parameter yang paling signifikan.
QUALITY CONTROL OF ROBUSTA GREEN BEANS USING NEW SEVEN QUALITY CONTROL TOOLS (CASE STUDY ON PTPN XII JEMBER REGENCY)
Authors: Yuli Wibowo  , Yuniarsih Handayani Rhoiyfah
Published: July 2023

This study aims to apply the new seven quality control tools (new 7QC tools) method in controlling the quality of robusta coffee beans in state-owned plantation companies. This research is a case study at PT. Perkebunan Nusantara XII, Silosanen Plantation, Jember Regency. Data processing uses the new 7QC tools method which consists of three stages, including (i) identification of quality problems using affinity diagrams and relationship diagrams; (ii) analysis of quality problems using tree diagrams, matrix diagrams, and matrix data analysis; (iii) planning quality improvement using arrow diagrams and process decision program charts. The results showed that quality 1 of robusta coffee beans which were considered defective were recorded at 21.81%. The results of the identification of the main cause of the emergence of defects in the coffee beans are the lack of supervision during the processing. The results of the analysis of quality problems indicate that improving the supervision of workers, can be started by focusing on each worker's tasks in improving worker performance, better environmental and material conditioning, method improvement, and optimization of machine use. The recommended improvement plans to improve supervision of workers include carrying out production activities according to a predetermined sequence and determining the number of workers according to production needs to facilitate supervision.
KELAYAKAN FINANSIAL DAN STRATEGI PENGEMBANGAN USAHATANI SAYURAN HIDROPONIK CV. LANGGENG HIDROPONIK KOTA KEDIRI
Authors: Kusmiati Ati , Evana Tasya Sendy , Fauziyah Diana
Published: July 2023

Kota Kediri merupakan salah satu kota yang mulai mengembangkan sayuran dengan sistem tanam hidroponik. Meskipun peluang sayuran hidroponik di Kota Kediri masih sangat terbuka lebar namun pada praktiknya banyak kendala yang dilalu dalam memulai usahatani sayuran dengan sistem hidroponik. Salah satunya adalah tingginya modal yang harus dikeluarkan untuk memulai usahatani sayuran hidroponik. Penelitian ini bertujuan untuk mengetahui: (1) kelayakan finansial usahatani sayuran hidroponik, (2) sensitivitas usahatani sayuran hidroponik terhadap penurunan produksi sebesar 10% dan peningkatan biaya produksi sebesar 20%, (3) strategi pengembangan usaha yang tepat untuk diterapkan pada CV. Langgeng Hidroponik Kota Kediri. Hasil penelitian ini menunjukkan bahwa : 1) usahatani sayuran hidroponik CV. Langgeng Hidroponik Kota Kediri layak untuk diusahakan dengan nilai NPV selama kurun waktu lima tahun positif, yaitu Rp77.886.534.77; dengan nilai Net B/C lebih dari 1 dengan nilai Net B/C lebih besar dari 1 (2,22); IRR lebih tinggi dari tingkat suku bunga (7%) yaitu 36,41%; dan PP lebih cepat dari umur ekonomis usahatani sayuran hidroponik (5 tahun) yaitu 3,5 tahun. 2) Hasil analisis sensitivitas menunjukkan bahwa usahatani sayuran hidroponik CV. Langgeng Hidroponik Kota Kediri sensitif terhadap penurunan produksi sebesar 10% dan kenaikan biaya produksi sebesar 20%. 3) Berdasarkan nilai TAS yang dihasilkan, maka strategi yang paling baik untuk diimplementasikan dan menjadi prioritas pertama pada usahatani sayuran hidroponik CV. Langgeng Hidroponik Kota Kediri adalah strategi pertama yaitu melakukan kerjasama dengan petani hidroponik lain secara intensif untuk meningkatkan kompetensi SDM.
Emotional intelligence as a resource in ensuring a person’s subjective well-being
Authors: Ichitovkina Elena G. , Zhernov Sergey V. , Zavadsky Alexander N.
Published: July 2023

Psychological and subjective well-being of a person is directly related to his emotional and behavioral health. Economic instability, growth of armed conflicts, terrorist acts and other social disasters have jeopardized the psychological well-being of a person and social society as a whole. To successfully adapt to modern living conditions in a rapidly changing society, it is necessary not only to deepen your knowledge and professional experience, but also to improve oneself as a person. In these conditions, emotional intelligence is one of the resources that ensure the development of personality and a factor contributing to the success of a person in various aspects of life, including in the professional sphere. Successful self-realization of a person is an integral part of self-improvement and human development. Emotional intelligence is often considered as one of the conditions contributing to the formation of human well-being as a whole: in a professional, family, personal context. In addition, emotional intelligence and human well-being are factors of stability and well-being of society. The comprehensive assessment of the problem of subjective well-being in conjunction with various levels of human activity, including emotional intelligence is as necessary as scientific development of issues related to overcoming stress with the formation of psychological and subjective well-being of the individual.
Prinsip dan Asas Masyarakat Multikultural Perspektif Tariq Ramadan
Authors: Saumantri Theguh
Published: July 2023

Pluralism and inclusivity are important aspects that need to be understood in the context of today’s multicultural society. However, in pluralistic societies, conflicts often arise due to differences and disagreements among different groups. This study aims to analyze the concepts of pluralism and inclusivity in the thoughts of Tariq Ramadan, with a focus on the concepts of European Muslim and Dar al-Shahadah. This research is a literature study with a philosophical approach that aims to examine the ideas and concepts of the figure. The results of this study indicate that in his thinking, Tariq Ramadan emphasizes the importance of a critical, inclusive, and pluralistic approach to Islam as well as advocating for reform in Islamic thought. His concepts of European Muslim and Dar al-Shahadah offer a positive view of the role of Muslim identity in an increasingly complex and changing global context in creating a more just and harmonious society and teaching the importance of recognizing and respecting the truth and human rights in all regions of the world, regardless of religious and cultural differences.
Ziarah Makam dan Ritual Besangi
Authors: . Nuruddin
Published: July 2023

This study aims to determine the motivation of the community behind the besangi ritual during the pilgrimage to the Maqam of Maulana Syeh Gauz Abdurrozak and also to find out what is felt after making the pilgrimage to the grave. In this re-search, the method used is qualitative research method. By using data collection techniques namely observation, interviews, documentation and data analysis. As for the results of research on grave pilgrimages at Wali Maulana Syeh Gauz Ab-durrozak's Tomb which is in Loang Baloq, namely as a form of respect to remember the services of the saints in the process of Islamization in Indonesia, especially in Lombok. In addition, the tomb of the guardian is a medium for conveying wishes to Allah and obtaining blessings from the wali.
Keywords: Ziarah, Makam, ritual, besangi, Lombok
Hubungan Tingkat Pendidikan terhadap Pengetahuan pada Pedagang Tahu Putih tentang Kandungan Formalin di Pasar Tradisional Kota Surabaya
Authors: Khanif Ashlikhatul , Mahmudiono Trias
Published: July 2023

Latar Belakang: Tahu menjadi makanan alternatif yang dikenal lezat dan bergizi, namun memiliki daya simpan yang singkat. Pedagang menggunakan formalin sebagai pengawet karena lebih hemat, lebih murah, dan mudah didapatkan. Formalin jika dikonsumsi terus menerus dapat menyebabkan gangguan kesehatan. Penggunaan formalin pada tahu dapat disebabkan karena kurangnya pengetahuan terhadap bahaya formalin. Pengetahuan seseorang dapat dipengaruhi oleh pendidikan seseorang.
Tujuan: Penelitian ini bertujuan untuk menganalisis hubungan tingkat pendidikan terhadap pengetahuan pada pedagang tahu putih tentang kandungan formalin di pasar tradisional Kota Surabaya
Metode: Penelitian dilaksanakan pada 31 Mei-13 Juni 2022 di pasar tradisional Surabaya. Jenis penelitian ini adalah observasional analitik dengan pendekatan cross-sectional. Sampel penelitian sebanyak 46 pedagang diambil menggunakan teknik multistage random sampling. Wawancara menggunakan kuesioner berisi 12 pertanyaan pilihan ganda. Uji kualitatif kandungan formalin menggunakan MQuant Formaldehyde Test. Analisis statistik menggunakan uji chi-square dengan signifikansi 0,05.
Hasil: Hasil penelitian ini menemukan bahwa sebagian besar pedagang memiliki tingkat pendidikan terakhir SD-SMP (60,9%) dan pengetahuan yang kurang mengenai formalin (41,3%). Hasil uji kualitatif menunjukkan tidak terdapat sampel tahu yang mengandung  formalin (100%).
Kesimpulan: Terdapat hubungan yang bermakna antara tingkat pendidikan terhadap tingkat pengetahuan pedagang tahu putih mengenai kandungan formalin di pasar tradisional Kota Surabaya (p=0,043).
The Relationship Between Family Smoking Habits and the Use of Room Perfume with Complaints of Allergic Rhinitis in Children Aged 13-14 Years in Rungkut District, Surabaya City
Authors: Fakhry Muhammad Aziz Al , Prasasti Corie Indria
Published: July 2023

ABSTRACT
 
Background: Allergic rhinitis is defined clinically as a symptom of nasal hypersensitivity triggered by a certain allergen present in the environment. The prevalence of allergic rhinitis has increased over the past 5 years. Indoor air quality is influenced by the number of pollutants according to the place, climate, culture, local ambient air, characteristics of buildings and indoor activities.
Objectives: To analyze the relationship between family smoking habits and room perfume use with complaints of allergic rhinitis in children aged 13-14 years in Rungkut District, Surabaya City.
Methods: This research method was quantitative research with an analytical type of observational research with a cross-sectional research design. The sample size in this study was 94 children aged 13-14 years and the sample was taken using systematic random sampling. Data collection by conducting interviews, observations and measurements directly. The test used is chi-square
Results: There was a relationship between family smoking habits and complaints of allergic rhinitis in children (p = 0.000) and there was a relationship between the use of room perfume and complaints of rhinitis in children (p = 0.029).
Conclusion: Family smoking habits and the use of room perfume have a significant relationship with complaints of allergic rhinitis in children. Families are encouraged to eliminate smoking and minimize the use of room perfume in order to minimize risk factors for complaints of allergic rhinitis in children.
Crystallographic variant mapping using precession electron diffraction data
Authors: H. Hansen Marcus
Published: July 2023

In this work, we developed three methods to map crystallographic variants of samples at the nanoscale by analyzing precession electron diffraction data using a high-temperature shape memory alloy and a VO2 thin film on sapphire as the model systems. The three methods are (I) a user-selecting-reference pattern approach, (II) an algorithm-selecting-reference-pattern approach, and (III) a k-means approach. In the first two approaches, Euclidean distance, Cosine, and Structural Similarity (SSIM) algorithms were assessed for the diffraction pattern similarity quantification. We demonstrated that the Euclidean distance and SSIM methods outperform the Cosine algorithm. We further revealed that the random noise in the diffraction data can dramatically affect similarity quantification. Denoising processes could improve the crystallographic mapping quality. With the three methods mentioned above, we were able to map the crystallographic variants in different materials systems, thus enabling fast variant number quantification and clear variant distribution visualization. The advantages and disadvantages of each approach are also discussed. We expect these methods to benefit researchers who work on martensitic materials, in which the variant information is critical to understand their properties and functionalities.
УДОСКОНАЛЕННЯ КОМП’ЮТЕРНОЇ МОДЕЛІ ДЛЯ ОБРОБКИ ТА АНАЛІЗУ СПЕКЛ-ІНТЕРФЕРОГРАМ КРОВІ ЛЮДИНИ
Authors: Байкова Анастасія , Богомолов Микола
Published: July 2023

Наразі досить багато захворювань людини діагностується шляхом оптичного дослідження змін в клітинній морфології, а особливо змін в морфології еритроцитів. Така тенденція спостерігається оскільки характерні зміни в клітинах крові можуть вказувати на наявність патологій в організмі людини. Серед оптичних методів дослідження параметрів крові людини, особливої уваги набув метод спекл-інтерферометрії, тому що він дозволяє досліджувати оптично шорсткі поверхні, до яких належить кров. Такий метод лазерного аналізу потребує обробки отриманих зображень для інтерпретації результатів дослідження. Існуючі компʼютерні моделі для обробки спекл-інтерферограм мають низку обмежень, зазвичай за кількістю аналізованих зображень, що ускладнює дослідження. В роботі представлено удосконалену компʼютерну модель для аналізу спекл-зображень крові, що не має обмежень за кількістю аналізованих зображень.
ЕКСПЕРИМЕНТАЛЬНИЙ КОМПЛЕКС ДЛЯ ДОСЛІДЖЕННЯ НЕЙРОФІЗІОЛОГІЧНИХ ХАРАКТЕРИСТИК ПРИ РІЗНИХ ФОРМАХ ТРЕНУВАННЯ ЛУЧНИКІВ
Authors: Литвякова Дарина , Овчаренко Ганна , Білошицька Оксана
Published: July 2023

В роботі запропоновано інструментальній метод кількісної оцінки психологічної підготовки спортсменів, а особливо – стрільців, оскільки було помічено, що при бездоганній ззовні техніці виконання пострілу поставлені результати не досягаються. Безліч помилок психологічного характеру знайдено та описано у всіх видах спорту, проте лише за наявності об’єктивних методів діагностики можна говорити про точні вказівки та інструкції при підготовці спортсменів. До цих пір виявлення помилок такого роду залежало лише від тренерського досвіду та таланту. Для об’єктивізації виявлення помилок запропоновано використовувати ЕЕГ. При наявності чіткого алгоритму щодо аналізу та використання ЕЕГ в цілому кожен спортсмен зможе самостійно зрозуміти свої помилки. Та й невірне тлумачення буде більш малоймовірним. Крім того, вивчення ЕЕГ дає змогу більш глибоко зрозуміти процеси, що відбуваються в спортсмені в момент виконання пострілу. Це може відкрити нові напрями удосконалення техніки, що, в свою чергу, дасть принципово новий результат.

В ході роботи було виконано візуальний аналіз ЕЕГ, домінуючих та суб-домінуючих ритмів у стрільців, а також картування. Проведено аналіз вкладів ритмів з допомогою критеріїв Краскела-Уолліса та Манна-Уітні. На основі отриманих результатів дано чіткі методи визначення шістьох основних помилок в стрільбі з луку (зацілювання, підловлювання точки прицілювання, очікування пострілу, втрата концентрації, невідповідність перенесення уваги з алгоритмом та невірне виконання ідеомоторного тренування). Знайдено «мічені ритми», за допомогою яких можна контролювати проходження уявного тренування. Також виявлено домінантність дельта- та тета-ритмів, що говорить про глибоку зажуреність спортсмена у відчуття власного тіла, а не навколишнього світу. При цьому не виявлено впливу прицілювання на роботу мозку, що говорить про паралельність цієї дії основному алгоритму
The Role of Hypothalamic Phospholipid Liposomes in the Supportive Therapy of Some Manifestations of Long Covid: Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and Brain Fog
Authors: Menichetti Francesco
Published: July 2023

Long Covid is a heterogeneous clinical condition in which Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and brain fog stand out among the different clinical symptoms and syndromes. The cerebral metabolic alterations and neuroendocrine disorders seem to constitute an important part of Long Covid. Given the substantial lack of drugs and effective therapeutic strategies, hypothalamic phospholipid liposomes which have been on the market for several years as adjuvant therapy of cerebral metabolic alterations resulting from neuroendocrine disorders, can be taken into consideration in an overall therapeutic strategy that aims to control the Long Covid associated symptoms and syndromes. Their pharmacological mechanisms and clinical effects strongly support their usefulness in Long Covid. Our initial clinical experience corroborates this rationale. Further research is imperative in order to obtain robust clinical evidence.
Research on an Intra-Pulse Orthogonal Waveform and Methods Resisting Interrupted-Sampling Repeater Jamming within the Same Frequency Band
Authors: Dai Huahua , Zhao Yingxiao , Su Hanning , Wang Zhuang , Bao Qinglong , Pan Jiameng
Published: July 2023

Abstract: Interrupted-sampling repeater jamming (ISRJ) is a kind of intra-pulse coherent decep-tion jamming that can generate false target peaks in the range profile and interfere with the de-tection and tracking of real targets. In this paper, an anti-ISRJ method based on the intra-pulse orthogonal waveform is proposed, which can recognize common interference signals by com-paring sub-signal matched filtering results. For some special scenes where real targets cannot be directly differentiated from false targets, a new recognition method based on the energy discon-tinuity of the interference signal in the time domain is proposed in this paper. The method pro-posed in this paper can recognize real and false targets in all ISRJ modes without any prior in-formation, such as jammer parameters, with a small amount of calculation, which is suitable for actual radar systems. Simulation experiments using different interference parameters show that although this method has a 3dB loss of pulse compression gain, it can completely suppress dif-ferent kinds of ISRJ interference when the SNR before pulse compression is higher than -20dB, with 100 % target detection probability.
Spatial Transcriptomic Analysis of Focal and Normal Areas of Myocyte Disarray in Human Hypertrophic Cardiomyopathy
Authors: Laird Jason , Perera Gayani , Batorsky Rebecca , Wang Hongjie , Arkun Knarik , Chin Michael T.
Published: July 2023

Hypertrophic Cardiomyopathy (HCM) is a common inherited disorder that can lead to heart failure and sudden cardiac death, characterized at the histological level by focal areas of myocyte disarray, hypertrophy and fibrosis, and few disease-targeted therapies exist. To identify, focal, spatially restricted alterations in transcriptional pathways and reveal novel therapeutic targets, we performed a spatial transcriptomic analysis of areas of focal myocyte disarray compared to areas of normal tissue, using a commercially available platform (GeoMx, nanoString). We analyzed surgical myectomy tissue from four patients with HCM and control interventricular septum tissue from two unused organ donor hearts that were free of cardiovascular disease. Histological sections were reviewed by an expert pathologist and 72 focal areas with varying degrees of myocyte disarray (normal, mild, moderate, severe) were chosen for analysis. Areas of interest were interrogated with the Human Cancer Transcriptome Atlas designed to profile 1800 transcripts. Differential expression analysis revealed significant changes in gene expression between HCM and Control tissue, and functional enrichment analysis indicated these genes were primarily involved in interferon production and mitochondrial energetics. Within HCM tissue, differentially expressed genes between areas of mild and moderate disarray were enriched for genes related to mitochondrial energetics (moderate disarray) and response to oxygen/cytokine levels (mild disarray). The comparison between areas of moderate and severe disarray were enriched for genes related to the c-Jun N-terminal kinase (JNK) cascade in severe disarray. Analysis of ligand-receptor pair gene expression revealed that HCM tissue exhibited downregulation of platelet-derived growth factor (PDGF), NOTCH, junctional adhesion molecule, and CD46 signaling, while showing upregulation of fibronectin, CD99, cadherin, and amyloid precursor protein signaling. A deconvolution analysis utilizing the matched single nuclei RNA-sequencing (snRNA-seq) data to determine cell type composition in areas of interest revealed significant differences in fibroblast and vascular cell composition in areas of severe disarray when compared to normal areas in HCM samples. Cell composition in normal areas from control tissue was also divergent from normal areas in HCM samples, which was consistent with the differential expression results. Overall, our data identify novel and potential disease-modifying targets for therapy in HCM.
Penerapan Analisis Jalur (Path Analysis) dalam Menentukan Faktor-faktor yang Mempengaruhi Angka Harapan Hidup di Wilayah Indonesia Bagian Tengah
Authors: Wahyuni Cucun
Published: July 2023

Penelitian ini dilakukan untuk mengidentifikasi faktor-faktor yang secara langsung atau tidak langsung mempengaruhi angka harapan hidup di wilayah Indonesia bagian tengah. Metode analisis yang digunakan adalah Analisis Jalur dengan menggunakan data sekunder dari BPS tahun 2017. Hasil pengujian kecocokan model diperoleh model yang sesuai dengan p-value chi-square 0,867, nilai The Root Mean Square Error of Approximation (RMSEA) yang cukup kecil yaitu 0,082, nilai Tucker Lewis Index (TLI) sebesar 0,950 dan nilai Comparative Fit Index (CFI) sebesar 0,990. Pengujian hipotesis menunjukkan bahwa variabel Rata-rata Lama Sekolah dan Pengeluaran per kapita secara signifikan berhubungan langsung dengan Angka Harapan Hidup. Selain itu, variabel Rata-rata Lama Sekolah juga secara tidak langsung mempengaruhi Angka Harapan Hidup melalui variabel Pengeluaran per kapita.
Pemodelan Harga Cabai Indonesia dengan Metode Seasonal ARIMAX
Authors: Faris Nasirudin  , Abdullah Ahmad dzikrullah
Published: July 2023

Chili is one of the plants favored by the people of Indonesia because Indonesian cuisine is famous for its spicy taste and spices in every food dish. The rise and fall of chili prices in the market are caused by chili farmers whose production decision-making processes are allegedly not handled and supported by a good production and price forecast. Therefore, analysis is needed to see the forecasting of chili prices in Indonesia in the future. The method that researchers use in forecasting in this study is the SARIMA and SARIMAX methods using the variables of rainfall, inflation, and google trend. The analysis shows that the SARIMAX method is the best model for predicting chili prices with a MAPE value of 6.889% compared to a MAPE SARIMA value of 7.630%.
Closed-Loop O2 Use During Invasive Mechanical Ventilation of Pediatric Patients (CLOUDIMPP): A Randomized Cross-Over Study
Authors: Atakul Gulhan , Ceylan Gokhan , Sandal Ozlem , Soydan Ekin , Topal Sevgi , Colak Mustafa , Hepduman Pinar , Karaarslan Utku , Zimmerman Jan , Novotni Dominik , Agin Hasan
Published: July 2023

Oxygen is an effective treatment for any type of respiratory failure. To prevent hypoxemia and hyperoxemia, the Pediatric mechanical ventilation consensus conference (PEMVECC) recommends monitoring oxygen saturation with pulse oximetry (SpO2) in all ventilated children and measuring partial arterial oxygen pressure (PaO2) in moderate to severe cases. However, this may require manual adjustments of inspired oxygen, which can be burdensome during high-demand periods such as the current COVID-19 epidemic. Additionally, the pandemic has highlighted the importance of optimizing oxygen use in hospitals as it may become a scarce resource during such high-demand periods. Previous studies have shown that automatic oxygen titration (AOT) is feasible in patients receiving respiratory support therapy, including those with mild acute hypoxemic respiratory failure and exercise-induced desaturation. This study aims to assess the safety and efficacy of a closed-loop FiO2 controller in comparison to traditional manual FiO2 regulation during invasive mechanical ventilation (IMV) in pediatric patients within a pediatric critical care unit (PICU). The hypothesis is that the closed-loop FiO2 controller will increase the time spent within clinically intended SpO2 ranges and decrease the time spent outside these ranges. The research is registered under NCT05714527
A systematic review and meta-analysis on endothelial dysfunction post-shock resuscitation
Authors: Obonyo Nchafatso , Sela Declan , Raman Sainath , Rachakonda Reema , Schneider Bailey , Hoe Louise See , Fanning Jonathon , Bassi Gianluigi Li , Maitland Kathryn , Suen Jacky , Fraser John
Published: July 2023

Introduction
Shock-induced endotheliopathy (SHINE), defined as a profound sympathoadrenal hyperactivation in shock states leading to endothelial activation, glycocalyx damage, and eventual compromise of end-organ perfusion, was first described in 2017. The aggressive resuscitation therapies utilised in treating shock states could potentially lead to further worsening endothelial activation and end-organ dysfunction.
Objective
This study aimed to systematically review the literature on resuscitation-associated and resuscitation-induced endothelial dysfunction.
Methods
A pre-determined structured search of literature published over an eleven-year period (1 January 2011 to 31 December 2022) was performed in two indexed databases (PubMed/MEDLINE and EMBASE) per PRISMA guidelines.
Results
Thirty studies met the inclusion criteria and were included in the final qualitative synthesis. Most of these studies (43%) reported on a combination of mediators released from endothelial cells and biomarkers of glycocalyx breakdown, while only 20% reported on microvascular flow changes. Only eight individual studies were included in the quantitative meta-analysis based on the comparability of the parameters assessed. Six studies measured syndecan-1, with a heterogeneity index, I2 = 75.87% (pooled effect-size; z = 2.00; mean = 0.43; 95% CI 0.01 to 0.85; p = 0.05). Thrombomodulin was measured in 4 comparable studies (I2 = 78.93%; z = 1.57; mean = 0.41; 95% CI -0.10 to 0.92; p = 0.12).
Conclusion
Acute resuscitation therapies administered could exacerbate endothelial dysfunction in shock states. In the included studies, syndecan-1 had the highest frequency of assessment in the post-resuscitation period and changes in concentrations showed a statistically significant effect of the resuscitation. There are inadequate data available in this area and further research and standardisation of the ideal assessment and panel of biomarkers are urgently needed.
DEAr – Differential Expression Analyzer
Authors: Karpenko Dmitriy
Published: July 2023

Differential expression of genes is one of the key features of modern biology for cells and tissues. To present differential expression from real time PCR data, the expression of housekeeping genes is considered as a baseline. There are solutions available to help verify that the genes provided are stable enough to be used as a reference. But sometimes, we struggle to find genes that are stable for the required cells and conditions. Here I present a program that simultaneously checks expressions of all genes and selects the most stable as a baseline for the less stable. Differential Expression Analyzer (DEAr) assigns weights to all values depending on accuracy of direct measurements and reproducibility of differential expressions in the dataset, at the same time the program utilizes external knowledge about stability or instability of genes in considered materials and contributes it to weights as well. The algorithm is based on recursive computations that are described in detail in the article. DEAr is packaged in an executable file for the OS Windows. DEAr takes and returns data in Excel format, so no programming skills are required for fast, automated analysis of differential expression.
Validation of the volumetric flow cytometry for bovine sperm concentration
Authors: Gloria Alessia , Bracco Claudia , Antenucci Emiliana , Contri Alberto
Published: July 2023

Sperm concentration is a stronghold of the andrological evaluation and the production of insemination doses. The use of haemocytometers, although considered the gold standard, is difficult to apply in field conditions because subjective and time-consuming. The present study was designed to validate the volumetric flow cytometry to estimate sperm concentration, comparing it with the performances of haemocytometer, NucleoCounter, and flow cytometry using fluorospheres. Compared with other methods, volumetric flow cytometry appeared less affected by large dilution of the sample, with similar concentrations calculated in the range of dilution 1:200 to 1:800. Using volumetric flow cytometry the population detected using morphological criteria and fluorescence of DNA better represents the real concentration of sperm in the sample. Volumetric flow cytometry showed high repeatability compared with the haemocytometer (coefficient of variation 1.85% and 4.52%, respectively). Finally, volumetric flow cytometry showed stable performances in cryopreserved samples analysis, with negligible effects of the medium components and cryoprotectants. The present study showed that volumetric flow cytometry is an accurate and precise method to estimate sperm concentration in bovine fresh and frozen semen, making possible the quantification of sperm functional subpopulation. This implements the possibility to study the relationship between sperm attributes and fertility.
The influence of reaction temperature and surfactant on the magnetic and catalytic properties of FeCo nanoparticles
Authors: Liao Tianfa , Wei Xiaohui , Xin Canjie , Yang Fujun
Published: July 2023

FeCo nanoparticles were prepared by controlling the reaction temperature and addition of surfactant. With the increasing of reaction temperature, CoFe2O4 impurities disappeared and crystalline FeCo nanoparticles were got. High Ms of 218.5 emu/g and low Hc of 90.2 Oe were observed after reacting at 150 oC. The best OER (oxygen evolution reaction) properties with low onset potential (0.73 V) and small Tafel value (105 mV/dec) were found in FeCo nanoparticles prepared at 150 oC. With increasing the surfactant ratio, the purity of FeCo particles did not change. However, the repulsive forces between the polar ends of oleic acid molecules induced the formation of uniform small FeCo nanoparticles. Large Ms of 166.4 emu/g and small Hc of 75.0 Oe were observed when the surfactant ratio was 3:1. The best OER activity with low onset potential (0.63 V) and small Tafel value (67 mV/dec) were found in FeCo nanoparticles prepared with the surfactant ratio of 3:1. The improved OER activity was contributed to the impurity reduction, the better crystallization, the larger surface area and the better magnetic properties, which could provide small electric resistance, more active sites and easy electron transfer.
Progesterone long-acting injection for assisted reproduction: Formulation and in vivo pharmacokinetic behavior
Authors: Li Li , Li Ming , Wei Wei , Yi Cong , Su Zhengxing , Zhao Dong
Published: July 2023

Progesterone (PG) is a key hormone that maintains the normal physiology of female reproductive system. However, due to its insolubility, the drugs used for assisted reproduction in clinical are mainly oil injections, patients with multiple injections are prone to adverse reactions such as redness, pain, induration and even muscle necrosis. In this study, we developed the progesterone long-acting injection which was administered every 7 days and could substantially reduce injection-site adverse reactions after long term administration. The progesterone long-acting injection containing 20% (w/v) progesterone was first prepared using solvent–antisolvent precipitation method. The particle size, X-ray diffraction (PXRD), differential scanning calorimetry (DSC) and in vitro dissolution were carried out to evaluate the characteristics. The results showed that the particle size of the progesterone long-acting injection was Dv10 8.128µm, Dv50 15.457µm, DV90 26.942µm and the crystal form was form α. In addition, the physical stability was also investigated after storage for 6 months at 40℃ and no obvious change in particle size was observed and the sample resuspension was well, indicating that the selected formulation was reasonable. The in vivo toxicology studies and pharmacokinetics tests showed that the progesterone long-acting injection had lower irritation than progesterone oil injection and could achieve sustained release for 7 days with a flat trend in plasma concentrations. Therefore, it was obvious that the progesterone long-acting injection we designed exhibited a lower muscle irritation and longer release period, which could be a better dosage form than progesterone oil injection and significantly increase patient compliance.
Recent innovations in fertilization with treated digestate from food waste to recover nutrients for arid agricultural fields
Authors: Skrzypczak Dawid , Trzaska Krzysztof , Mironiuk Małgorzata , Mikula Katarzyna , Izydorczyk Grzegorz , Polomska Xymena , Wiśniewski Jerzy , Mielko Karolina , Moustakas Konstantinos , Chojnacka Katarzyna
Published: July 2023

This study aims to explore the development of sustainable fertilizers from waste materials of a biogas plant and a brewery. These wastes, rich in organic carbon and nitrogen, were processed with sulfuric(VI) and phosphoric(V) acid mixture, facilitating the production of free amino acids and achieving waste sanitization. This treatment produced by-products, which extended the range of possible applications. The highest concentration of free amino acids (360 mg/L) was achieved through hydrolyzing with a 40% concentration medium over 24 hours. The study presents two fertilizer technologies, with and without micronutrients, that satisfy European Parliament Regulation 2019/1009 (Ntot>2%, Norg>0.5%, Corg>3%). Bioavailability of nutrients in the formulations ranged from 60% to 100%. The efficacies of these fertilizers were evaluated in 30-day pot trials with various plant species, with both single application and fertigation tested. Multielement analysis confirmed high nutrient transfer in the soil-plant system, and the inclusion of micronutrients led to biofortification of plant biomass in Cu, Mn, Zn, and Fe. These sustainable fertilizers present an alternative to traditional, non-renewable fertilizers and offer promising solutions for precision agriculture and environmentally conscious production.
Association of urinary bisphenols with thyroid function in the general population: a cross-sectional study of an industrial park in China
Authors: Hu Yang , Lai Shiming , Li Ying , Wu Xiaodong , Xing Mingluan , Li Xueqing , Xu Dandan , Chen Yuan , Xiang Jie , Cheng Ping , Wang Xiaofeng , Chen Zhijian , Ding Hao , Xu Peiwei , Lou Xiaoming
Published: July 2023

Background: Bisphenols (BPs) are potential thyroid disruptors that are widely used in many consumer products, leading to their widespread exposure in the general population. Current studies have found that there is a certain association between exposure to BPs and serum thyroid function measures, albeit with some controversy.
Objectives: To describe demographic characteristics, BP exposure levels, and thyroid function measurements in potentially exposed and control districts and to investigate the association of urinary BPs with thyroid function.
Methods: Data were collected from a general population aged 3–79 years (N = 281) recruited by the Zhejiang Human Biomonitoring Program (ZJHBP). The concentrations of 10 kinds of BPs in urine and serum free triiodothyronine (FT3), total triiodothyronine (TT3), free thyroxine (FT4), total thyroxine (TT4), thyroid-stimulating hormone (TSH), thyroglobulin (Tg), thyroglobulin antibodies (TgAb), thyroid peroxidase antibodies (TPOAb), and thyrotropin receptor antibody (TRAb) in serum were measured.
Results: Bisphenol A (BPA), bisphenol S (BPS), and bisphenol P (BPP) were detected, respectively, in 82.73%, 94.24%, and 55.40% of the population in the exposed area and 81.69%, 61.27%, and 43.66% of the population in the control area. Among adult females, serum TT3 was negatively associated with urinary BPA (β = –0.030, P = 0.047). Among minor females, FT4 and Tg levels were negatively associated with the urinary BPA (β = −0.026, P = 0.032 for FT4; β = −0.129, P = 0.035 for Tg), and TPOAb was positively associated with urinary BPA (β = 0.104 P = 0.039).
Conclusion: We found widespread exposure to BPA, BPS, and BPP in the general population of Zhejiang province and found a limited association between BPA and thyroid hormones. This association is gender- and age-dependent and needs to be confirmed in further studies.
Synergistic remediation of e-waste contaminated soil using thermally treated sludge residue and Lolium perenne L.
Authors: Gu Weihua , Bai Jianfeng , Chen Jianmeng
Published: July 2023

Electronic waste (e-waste) generation has significantly increased because of the widespread use of electronic products, and thermally treated sludge residue improves e-waste-contaminated soil by synergizing with Lolium perenne L. This study investigated the effects of different doses of thermally treated sludge residue (0% and 5%) in synergy with L. perenne L. on organic matter, nutrients, heavy metal morphology, and heavy metal uptake and transport by L. perenne L. in e-waste-contaminated soil using pot experiments. The results showed that the thermally treated sludge residue improved soil quality and significantly increased soil conductivity, cation exchange, organic matter, effective phosphorus, and fast-acting potassium content. New heavy metal crystalline phases were detected, and soil solubility humus components were found. Sludge fermentation residue significantly increased dissolved organic matter content, whereas sludge pyrolysis residue showed no significant effect. The combination of thermally treated sludge residue and L. perenne L. increased steady-state proportions of Cu, Zn, Pb, and Cd. The thermally treated sludge residue promoted L. perenne L. growth, increasing fresh weight, plant height, phosphorus uptake, and potassium uptake of L. perenne L.; it significantly reduced L. perenne L. response to Cu, Zn, Pb, and Cd. In addition, L. perenne L. promoted the activity of microorganisms in thermally treated sludge residue and improved its ability to reduce heavy metals. This approach has the potential for ecological remediation of contaminated sites, providing a new method for the remediation of e-waste-contaminated soil.
Pollination services are resilient to an extreme climatic event and mitigate crop yield declines
Authors: Devkota Kedar , Santos Charles F. dos , Nicholson Charlie C. , Egan Paul Anthony
Published: July 2023

Extreme climatic events pose a threat to pollinators and their ecosystem services to agriculture. Yet little remains known on how pollinator communities respond to many climate shocks and experimental studies on crop pollination remain worryingly absent. Here we examined the effects of an extreme flooding event on pollinator communities in Nepal, and conducted insect exclusion experiments to quantify potential impacts on pollination services to two globally-important crops (oilseed mustard and buckwheat). We found that flooding led to lower pollinator abundance as a whole and significantly altered community composition. While social bees (Apis florea, A. cerana, A. dorsata, and A. mellifera) did not show large changes, flooding effects were instead stronger and more consistent for solitary bee species – and in particular mining and ground nesting guilds (Halictus, Lasioglossum, Andrena spp.). Despite these impacts, pollinator services to both crops remained resilient to flooding. Although buckwheat in particular incurred significant yield loss due to flooding, due to the resilience of pollination services pollinators made a proportionally greater contribution to yield in flooded fields (54.1% of yield) than in non-flooded fields (36.1%). Our study highlights how extreme events such as flooding can disrupt pollinator communities, but also how pollination services can still show resilience to such shocks, and buffer against crop yield loss. Accordingly, we discuss the need to incorporate pollination into strategies for agricultural climate adaptation and disaster risk reduction, and call for more knowledge on the response of pollination services to extreme climatic events – in particular for smallholder farming systems.