bluuebunny/crossref_metadata_2025 huggingface dataset:
Preparation of microgel co-loaded with nuciferine and epigallocatechin-3-gallate for the regulation of lipid metabolism
Authors: Zhu Shengnan , Xu Weijia , Liu Jun , Guan Feng , Xu Aichun , Zhao Jin , Ge Jian
Published: December 2022

This study aims to enhance the stability and bioavailability of nuciferine (NF) and epigallocatechin-3-gallate (EGCG) by loading NF into liposomes and then incorporating the liposomes and EGCG into porous microgels (NFEG-microgel) prepared with chitosan and proanthocyanidin. Analysis of particle size (0.5–3.0 μm), electron microscopy, rheology, stability, and simulated gastrointestinal release confirmed that the prepared microgels had high encapsulation rate and good stability and release characteristics. Intervention experiments were performed by orally administering NFEG-microgel to high-fat diet rats to evaluate its efficacy and regulatory mechanism for blood lipid metabolism. NFEG-microgel intervention significantly reduced the body weight and serum lipid level, and the mechanism was related to the expression regulation of key genes involved in lipid metabolism and miRNAs (miR-126a-5p and miR-30b-5p) in serum extracellular vesicles. In addition, NFEG-microgel improved the diversity of gut microbiota by enriching short-chain fatty acids (SCFA)-producing bacteria and reducing harmful bacteria, suggesting that it can ameliorate lipid metabolism by regulating the intestinal flora community in rats.
Encapsulation of calcium carbonate with a ternary mixture of sodium caseinate/gelatin/xanthan gum to enhance the dispersion stability of solid/oil/water emulsions
Authors: Zhang Jie , Cao Yanping , Xu Duoxia
Published: December 2022

Calcium carbonate (CaCO3) has poor suspension stability, which severely limits its application in food processing and products. The solid/oil/water (S/O/W) emulsion stabilized by sodium caseinate (NaCas), gelatin (GEL), and xanthan gum (XG) ternary composite was to improve the dispersion stability of CaCO3 in emulsions. Particle size, Zeta potential, physical stability, and microstructure were determined to characteristic the stability of the S/O/W emulsions. Shear rheological and tribological analyses were used to characterize the rheological properties of S/O/W emulsions. X-ray diffraction (XRD), Infrared spectral analysis (FTIR), and molecular docking were used to characterize the molecular interactions, which was to explore the influence of the W phase on the system stability. It was found that when the NaCas concentration was 2 wt% and the S/O phase addition was 5%, the particle size distribution was uniform, and the physical stability was improved. CLSM and Cryo-SEM results showed that the S/O/W emulsions could embedded CaCO3 in the system, and formed a dense three-dimensional network space structure. The viscosity of the system increased and even agglomeration occurred with NaCas concentration increased, and the stability of the emulsion decreased. XRD results confirmed that the CaCO3 was partially covered due to physical embedding. Infrared spectral analysis and molecular docking results showed electrostatic, hydrophobic interaction, and hydrogen bond interaction between NaCas, GEL, and XG, which could improve the stability of S/O/W emulsions. The results showed that the S/O/W emulsions delivery system is an effective way to promote the application of CaCO3.
Emerging toolset of three-dimensional pulmonary cell culture models for simulating lung pathophysiology towards mechanistic elucidation and therapeutic treatment of SARS-COV-2 infection
Authors: Ni Kai , Che Bo , Yang Chongxin , Qin Youyuan , Gu Rong , Wang Chunhong , Luo Mingzhi , Deng Linhong
Published: December 2022

The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) poses a never before seen challenge to human health and the world economy. However, it is difficult to widely use conventional animal and cell culture models in understanding the underlying pathological mechanisms of COVID-19, which in turn hinders the development of relevant therapeutic treatments, including drugs. To overcome this challenge, various three-dimensional (3D) pulmonary cell culture models such as organoids are emerging as an innovative toolset for simulating the pathophysiology occurring in the respiratory system, including bronchial airways, alveoli, capillary network, and pulmonary interstitium, which provide a robust and powerful platform for studying the process and underlying mechanisms of SARS-CoV-2 infection among the potential primary targets in the lung. This review introduces the key features of some of these recently developed tools, including organoid, lung-on-a-chip, and 3D bioprinting, which can recapitulate different structural compartments of the lung and lung function, in particular, accurately resembling the human-relevant pathophysiology of SARS-CoV-2 infection in vivo. In addition, the recent progress in developing organoids for alveolar and airway disease modeling and their applications for discovering drugs against SARS-CoV-2 infection are highlighted. These innovative 3D cell culture models together may hold the promise to fully understand the pathogenesis and eventually eradicate the pandemic of COVID-19.
Association between different concentrations of human serum albumin and 28-day mortality in intensive care patients with sepsis: A propensity score matching analysis
Authors: Xu Weigan , Huo Jianyang , Cheng Guojun , Fu Juan , Huang Xiangqing , Feng Jinxia , Jiang Jun
Published: December 2022

Background: Human serum albumin (HSA) is a commonly used medication for the treatment of sepsis. However, there is no conclusive evidence as to whether different concentrations of HSA are associated with patient prognosis. This study aimed to evaluate the association between different concentrations of HSA and 28-day mortality in patients with sepsis.

Methods: The data for this retrospective study were collected from the Medical Information Mart for Intensive Care IV database. Patients with sepsis were divided into two groups according to the concentration of HSA received: 25% and 5% HSA. The primary outcome of this study was the 28-day mortality in patients with sepsis. To ensure the robustness of our findings, we used multivariate Cox regression, propensity score matching, double-robust estimation, and inverse probability weighting models.

Results: A total of 76,943 patients were screened, of whom 5,009 were enrolled. 1,258 and 3,751 patients received 25% and 5% HSA, respectively. The 28-day mortality rate was 38.2% (481/1,258) for patients in the 25% HSA group and 8.7% (325/3,751) for patients in the 5% HSA group. After propensity score matching, 1,648 patients were identified. The inverse probability weighting model suggested that 5% HSA received was associated with lower 28-day mortality (hazard ratio [HR]: 0.63, 95% confidence interval [CI]: 0.54–0.73, p < 0.001). Subgroup and sensitivity analysis confirmed the robustness of the results.

Conclusion: In patients with sepsis, 5% HSA received may be associated with a lower risk of 28-day mortality than 25% HSA. Further randomized controlled trials are required to confirm this association.
Plasma levels of platelet-enriched microRNAs change during antiplatelet therapy in healthy subjects
Authors: Krammer Teresa L. , Kollars Marietta , Kyrle Paul A. , Hackl Matthias , Eichinger Sabine , Traby Ludwig
Published: December 2022

Platelets are the main effectors of primary hemostasis but also cause thrombosis in pathological conditions. Antiplatelet drugs are the cornerstone for the prevention of adverse cardiovascular events. Monitoring the extent of platelet inhibition is essential. Currently available platelet function tests come with constraints, limiting use in antiplatelet drug development as well as in clinical routine. With this study, we aim to investigate whether plasma miRNAs might be suitable biomarkers for monitoring antiplatelet treatment. Platelet-poor plasma was obtained from a trial including 87 healthy male volunteers that either received ticagrelor (n = 44) or clopidogrel (n = 43). Blood was collected before drug intake and after 2 h, 6 h, and 24 h. We measured a panel of 11 platelet-enriched miRNAs (thrombomiRs) by RT-qPCR and selected four biomarker candidates (i.e., miR-223-3p, miR-150-5p, miR-126-3p, miR-24-3p). To further characterize those miRNAs, we performed correlation analyses with the number of extracellular vesicles and clotting time dependent on procoagulant vesicles (PPL assay). We show that platelet-enriched miRNAs in the circulation are significantly reduced upon P2Y12-mediated platelet inhibition. This effect occurred fast, reaching its peak after 2 h. Additionally, we demonstrate that higher baseline levels of thrombomiRs are linked to a stronger reduction upon antiplatelet therapy. Finally, we show that miRNAs from our panel might be the cargo of platelet-derived and procoagulant vesicles. In conclusion, we provide evidence that thrombomiR levels change within 2 h after pharmacological platelet inhibition and circulate the body within platelet-derived and procoagulant extracellular vesicles, rendering them potential biomarker candidates for the assessment of in vivo platelet function.
A high-efficiency PEG-Ca2+-mediated transient transformation system for broccoli protoplasts
Authors: Yang Dongxu , Zhao Yongyu , Liu Yumei , Han Fengqing , Li Zhansheng
Published: December 2022

Transient transformation of plant protoplasts is an important method for studying gene function, subcellular localization and plant morphological development. In this study, an efficient transient transformation system was established by optimizing the plasmid concentration, PEG4000 mass concentration and genotype selection, key factors that affect transformation efficiency. Meanwhile, an efficient and universal broccoli protoplast isolation system was established. Using 0.5% (w/v) cellulase R-10 and 0.1% (w/v) pectolyase Y-23 to hydrolyze broccoli cotyledons of three different genotypes for 3 h, the yield was more than 5×106/mL/g, and the viability was more than 95%, sufficient to meet the high standards for protoplasts to be used in various experiments. The average transformation efficiency of the two plasmid vectors PHG-eGFP and CP507-YFP in broccoli B1 protoplasts were 61.4% and 41.7%, respectively. Using this system, we successfully performed subcellular localization of the products of three target genes (the clubroot resistance gene CRa and two key genes regulated by glucosinolates, Bol029100 and Bol031350).The results showed that the products of all three genes were localized in the nucleus. The high-efficiency transient transformation system for broccoli protoplasts constructed in this study makes it possible to reliably acquire high-viability protoplasts in high yield. This research provides important technical support for international frontier research fields such as single-cell sequencing, spatial transcriptomics, plant somatic hybridization, gene function analysis and subcellular localization.
A multiple baseline trial of adapted prolonged exposure psychotherapy for individuals with early phase psychosis, comorbid substance misuse, and a history of adversity: A study protocol
Authors: Patterson Victoria C. , Tibbo Philip G. , Stewart Sherry H. , Town Joel , Crocker Candice E. , Ursuliak Zenovia , Lee Siranda , Morrison Jason , Abidi Sabina , Dempster Kara , Alexiadis Maria , Henderson Neal , Pencer Alissa
Published: December 2022

Background

Adversity is prevalent among people with psychotic disorders, especially those within the first 5 years of a psychotic disorder, called early phase psychosis. Although adversity can lead to many negative outcomes (e.g., posttraumatic stress symptoms), very few treatments for adversity-related sequelae have been tested with individuals with psychotic disorders, and even fewer studies have specifically tested interventions for people in early phase psychosis. Furthermore, people who misuse substances are commonly excluded from adversity treatment trials, which is problematic given that individuals with early phase psychosis have high rates of substance misuse. For the first time, this trial will examine the outcomes of an adapted 15-session prolonged exposure protocol (i.e., PE+) to observe whether reductions in adversity-related psychopathology occurs among people with early phase psychosis and comorbid substance misuse.

Methods

This study will use a multiple-baseline design with randomization of participants to treatment start time. Participants will complete baseline appointments prior to therapy, engage in assessments between each of the five therapy modules, and complete a series of follow-up appointments 2 months after the completion of therapy. Primary hypothesized outcomes include clinically significant reductions in (1) negative psychotic symptoms measured using the Positive and Negative Syndrome Scale, (2) adversity-related sequelae measured using the Trauma Symptom Checklist-40, and (3) substance use frequency and overall risk score measured with the Alcohol, Smoking, and Substance Involvement Screening Test. We also anticipate that clinically significant reductions in hopelessness and experiential avoidance, measured with the Beck Hopelessness Scale and Brief Experiential Avoidance Questionnaire, the theorized mechanisms of change of PE+, will also be observed. A secondary outcome is a hypothesized improvement in functioning, measured using the Clinical Global Impression and Social and Occupational Functioning Assessment scales.

Discussion

The results of this treatment trial will contribute to the advancement of treatment research for individuals in early phase psychosis who have current substance misuse and a history of adversity, and the findings may provide evidence supporting the use of hopelessness and experiential avoidance as mechanisms of change for this treatment.

Clinical trial registration

Clinicaltrials.gov, NCT04546178; registered August 28, 2020, https://clinicaltrials.gov/ct2/show/NCT04546178?term=NCT04546178&draw=2&rank=1.
Full-information item bifactor model for mathematical ability assessment in Chinese compulsory education quality monitoring
Authors: Meng Xiangbin , Yang Tao , Shi Ningzhong , Xin Tao
Published: December 2022

This study focuses on the measurement of mathematical ability in the Chinese Compulsory Education Qualification Monitoring (CCEQM) framework using bifactor theory. First, we propose a full-information item bifactor (FIBF) model for the measurement of mathematical ability. Second, the performance of the FIBF model is empirically studied using a data set from three representative provinces were selected from CCEQM 2015–2017. Finally, Monte Carlo simulations are conducted to demonstrate the accuracy of the model evaluation indices and parameter estimation methods used in the empirical study. The obtained results are as follows: (1) The results for the four used model selection indices (AIC, SABIC, HQ, BIC) consistently showed that the fit of the FIBF model is better than that of the UIRT; (2) All of the estimated general and domain-specific abilities of the FIBF model have reasonable interpretations; (3) The model evaluation indices and parameter estimation methods exhibit excellent accuracy, indicating that the application of the FIBF model is technically feasible in large-scale testing projects.
Interactivity in learning instructional videos: Sending danmaku improved parasocial interaction but reduced learning performance
Authors: Mou Ya , Jing Bin , Li Yichun , Fang Nanyang , Wu Changcheng
Published: December 2022

Introduction

The instructional video is considered to be one of the most distinct and effective virtual learning tools. However, one of its biggest drawbacks is the lack of social interaction that occurs. This study tested the impact of participants sending zero danmaku (sending messages on the screen), three danmaku sending, and unlimited danmaku as an instructional video plays on learning performance.

Methods

We assessed learners’ retention and transfer scores, as well as self-report scores for cognitive load and parasocial interaction. This study sample comprised 104 participants who were randomly assigned to learn from one of three instructional videos on the topic of the heart.

Results

The results showed that sending danmaku improved learners’ parasocial interaction, while significantly increasing their cognitive load and also hindering learning performance. The observed increase in cognitive load reported by learners was also caused by increased levels of parasocial interaction.

Discussion

Our findings suggest that by sending danmaku, learners can promote interactive learning, but that this has a negative impact on learning performance and the process of video learning.
Does the eight-factor “power to live” in disaster exist since childhood?
Authors: Matsuzaki Yutaka , Ishibashi Ryo , Yasuda Mari , Tanabe-Ishibashi Azumi , Honda Akio , Abe Tsuneyuki , Sugiura Motoaki
Published: December 2022

Background

Studies on the survivors of the 2011 Great East Japan earthquake and tsunami have revealed eight factors, called power to live, which are closely related to resilience and effective coping after intense and prolonged stress. However, whether the eight factors, which were examined in adults, are applicable to children is unclear. The purpose of this study is to evaluate whether the eight-factor structure of power to live was present since late childhood.

Method

A 34-item power to live questionnaire was filled by middle- to upper-grade elementary (n = 378) and junior high school students (n = 456). Moreover, because elementary school students may lack introspective ability, their power to live was evaluated through a parental assessment (n = 358). Additionally, we examined the relationship between each power to live factor and questions regarding disaster prevention awareness among 25 elementary school students.

Results

The results from confirmatory factor analysis for factor structure revealed generally acceptable fit indices. The reports from elementary school students and their parents significantly positively correlated with each power to live factor. Although reliability indices for factors such as stubbornness, etiquette, self-transcendence, and active well-being were not good for elementary school students, the reliability indices for all factors, excluding stubbornness, increased in junior high school students. Moreover, we identified a correlation between problem-solving, altruism, and emotional regulation and questionnaire items regarding awareness of disaster prevention in elementary school students.

Conclusion

Our results suggest that although factors common to adults, such as leadership, problem-solving, altruism, and emotional regulation, were identified at the elementary school stage, some factors, such as stubbornness, are in the process of being formed. Future studies should examine the developmental changes assumed to underlie these factors and their relationship to experience and neurodevelopmental basis.
General health status and psychological impact of COVID19 pandemic and curfew on children aging 3 to 12 years
Authors: Shamsah Abdullah , Aburezq Maryam , Abdullah Zahraa , Khamissi Fatemeh , Almaateeq Batool , AlAlban Fatima , Almashmoom Sarah , Ziyab Ali
Published: December 2022

Background

COVID-19 is an infectious disease that was declared as a pandemic and public health emergency in late 2019 and has impacted children's mental health worldwide. This study aimed to assess the general and mental health status of children during different stages of COVID-19 pandemic, and to identify the associated factors.

Methods

A cross-sectional study conducted on children aging 3 to 12 years in Kuwait during three different stages of COVID19 pandemic (pre-total curfew, during total curfew, and post-total curfew). The psychological status was assessed using the fifth edition of the Diagnostic and Statistical Manual of Mental Disorders criteria.

Results

Of 2157 children between the age of 3 to 12 years old, 853 (39.5%) reported increased level of aggression, 789 (36.6%) over-crying, 749 (34.7%) sadness, 493 (22.9%) anxiety, 429 (19.9%) anhedonia, 383 (17.8%) confusion, 274 (12.7%) nightmares, 177 (8.2%) avoidance, 174 (8.1%) physical symptoms and 121 (5.6%) bedwetting during the pandemic. General health status was also affected with reported disturbed sleeping pattern (84.6%), altered appetite (50.9%) and weight changes (36.9%), mainly weight gain. Risk factors included being non-national, as well as having lower parental educational level and lower socioeconomic status; while protective factors involved meeting classmates, indoor and outdoor activities, and less screen time.

Conclusions

COVID19 crisis had drastic impact on children's mental and general health, requiring serious action regarding evaluating this generation and intervening accordingly.
Socioecological model-based design and implementation principles of lower limb preservation programs as partners for limb-loss rehabilitation programs— A mini-review
Authors: Khetarpaul Vipul , Kirby John P. , Geraghty Patrick , Felder John , Grover Prateek
Published: December 2022

People with lower limb loss, especially of dysvascular etiology, are at substantial risk for both ipsilateral and contralateral reamputation. Additionally, while not as well documented for reamputation, there is recognition that amputation incidence is influenced by not only sociodemographic factors such as sex, race, socioeconomic status, but also by system factors such as service access. A systems strategy to address this disparity within the field of limb-loss rehabilitation is for Limb-loss Rehabilitation Programs (LRP) to partner with medical specialists, mental health professionals, and Limb Preservation Programs (LPP) to provide comprehensive limb care. While LPPs exist around the nation, design principles for such programs and their partnership role with LRPs are not well established. Using a socioecological model to incorporate hierarchical stakeholder perspectives inherent in the multidisciplinary field of limb care, this review synthesizes the latest evidence to focus on LPP design and implementation principles that can help policymakers, healthcare organizations and limb-loss rehabilitation and limb-preservation professionals to develop, implement, and sustain robust LPP programs in partnership with LRPs.
Assessing UAV-based laser scanning for monitoring glacial processes and interactions at high spatial and temporal resolutions
Authors: Baurley Nathaniel R. , Tomsett Christopher , Hart Jane K.
Published: December 2022

Uncrewed Aerial Vehicles (UAVs), in combination with Structure from Motion (SfM) photogrammetry, have become an established tool for reconstructing glacial and ice-marginal topography, yet the method is highly dependent on several factors, all of which can be highly variable in glacial environments. However, recent technological advancements, related primarily to the miniaturisation of new payloads such as compact Laser Scanners (LS), has provided potential new opportunities for cryospheric investigation. Indeed, UAV-LS systems have shown promise in forestry, river, and snow depth research, but to date the method has yet to be deployed in glacial settings. As such, in this study we assessed the suitability of UAV-LS for glacial research by investigating short-term changes in ice surface elevation, calving front geometry and crevasse morphology over the near-terminus region of an actively calving glacier in southeast Iceland. We undertook repeat surveys over a 0.1 km2region of the glacier at sub-daily, daily, and weekly temporal intervals, producing directly georeferenced point clouds at very high spatial resolutions (average of >300 points per m−2at 40 m flying height). Our data has enabled us to: 1) Accurately map surface elevation changes (Median errors under 0.1 m), 2) Reconstruct the geometry and evolution of an active calving front, 3) Produce more accurate estimates of the volume of ice lost through calving, and 4) Better detect surface crevasse morphology, providing future scope to extract size, depth and improve the monitoring of their evolution through time. We also compared our results to data obtained in parallel using UAV-SfM, which further emphasised the relative advantages of our method and suitability in glaciology. Consequently, our study highlights the potential of UAV-LS in glacial research, particularly for investigating glacier mass balance, changing ice dynamics, and calving glacier behaviour, and thus we suggest it has a significant role in advancing our knowledge of, and ability to monitor, rapidly changing glacial environments in future.
Exploring marine mammal presence across seven US national marine sanctuaries
Authors: DeAngelis Annamaria I. , Van Parijs Sofie M. , Barkowski Jack , Baumann-Pickering Simone , Burger Kourtney , Davis Genevieve E. , Joseph John , Kok Annebelle C. M. , Kügler Anke , Lammers Marc , Margolina Tetyana , Pegg Nicole , Rice Ally , Rowell Timothy J. , Ryan John P. , Stokoe Allison , Zang Eden , Hatch Leila
Published: December 2022

The United States of America’s Office of National Marine Sanctuaries (ONMS) hosts 15 National Marine Sanctuaries (NMS) and two Monuments in its waters. Charismatic marine megafauna, such as fin whales (Balaenoptera physalus), humpback whales (Megaptera novaeangliae), and various delphinid species frequent these areas, but little is known about their occupancy. As part of a national effort to better understand the soundscapes of NMS, 22 near-continuous passive acoustic bottom mounted recorders and one bottom-mounted cable hydrophone were analyzed within seven NMS (Stellwagen Bank, Gray’s Reef, Florida Keys, Olympic Coast, Monterey Bay, Channel Islands, and Hawaiian Islands Humpback Whale sanctuaries). The daily acoustic presence of humpback and fin whales across 2 years (November 2018–October 2020) and hourly presence of delphinids over 1 year (June 2019–May 2020) were analyzed. Humpback whales showed variability in their acoustic presence across NMS, but in general were mostly present January through May and September through December, and more scarce or fully absent June through August. Consecutive days of humpback whale vocalizations were greatest at sites HI01 and HI05 in the Hawaiian Islands Humpback Whale NMS and fewest at the Channel Islands NMS. Fin whales exhibited a similar seasonal pattern across the West Coast NMS and Stellwagen Bank NMS. Monterey Bay NMS had the greatest number of median consecutive presence of fin whales with fewest at Stellwagen Bank NMS. Delphinid acoustic presence varied throughout and within NMS, with sites at the Channel Islands and Hawaiʻi NMS showing the highest occupancy. All NMS showed distinct monthly delphinid acoustic presence with differences in detected hours between dayversusnight. Sixteen sites had medians of delphinid presence between one and three consecutive days, while three sites had 5 days or more of consecutive presence, and one site had no consecutive delphinid presence, showing clear variation in how long they occupied different NMS. Marine mammals utilized all NMS and showed a wide range of occupancy, emphasizing the importance of understanding species use across different NMS as biological areas for migration, breeding and foraging.
Quercetin ameliorates chicken quality by activating the PI3K/PKB/AMPK signaling pathway in broilers
Authors: Wang Mi , Wang Bo , Zhou Shuaishuai , Liu Jiayan , Lu Han , Wu Hao , Ding Manyi , Li Yao
Published: December 2022

This study was conducted to investigate the effects and mechanism of quercetin on chicken quality in broilers. We selected 480 AA broilers (1 day old) and randomly allotted those to four treatments (negative control and 0.2, 0.4, or 0.6 g of quercetin per kg of diet) for 42 days. Compared with the control group, the supplementation with 0.4 g of quercetin significantly increased the pH45min and L* value of the thigh muscle and decreased the shearing force of the thigh muscle and breast muscle and drip loss of the thigh muscle (P < 0.05). The supplementation with 0.6 g/kg of quercetin significantly increased the pH45min and L* value of the thigh muscle, and pH45min of breast muscle and decreased the drip loss of the thigh muscle (P < 0.05). Sensory scores of meat color, tenderness, and juiciness also were improved with increasing quercetin concentration (P < 0.05). The inosinic acid (IMP) content of the breast and thigh muscles of broilers was significantly increased by supplementation with 0.6 g/kg of quercetin (P < 0.05). Supplementation with 0.2, 0.4, and 0.6 g of quercetin significantly reduced mRNA expression of L-FABP (P < 0.05, P < 0.05, and P < 0.05); supplementation with 0.4 and 0.6 g/kg of quercetin significantly increased mRNA expression of PKB and AMPKα1 (P < 0.05 and P < 0.05); supplementation with 0.6 g/kg of quercetin in the diet significantly reduced mRNA expression of SREBP1 and HMGR (P < 0.05 and P < 0.05) and significantly increased mRNA expression of CPT1 and PPARγ (P < 0.05 and P < 0.05); and supplementation with 0.2, 0.4, and 0.6 g/kg of quercetin significantly increased mRNA expression of PI3K, LPL, and Apo A1 and significantly reduced mRNA expression of ACC and FATP1 in the breast muscle of broilers (P > 0.05). PI3k, PKB, AMPK, SREBP1, and L-FABP were significantly and positively correlated with pH45min (P < 0.05); PPARγ was significantly and positively correlated with shear force (P < 0.05); CPT1 was significantly and positively correlated with the L* value (P < 0.05); and HMGR was significantly and positively correlated with drip loss (P < 0.05). In conclusion, quercetin improved the meat quality, protecting it against lipid oxidation and deposition by regulating the PI3K/PKB/AMPKα1 signaling pathway in the breast muscle of broilers.
Robust Attitude Control of an Agile Aircraft Using Improved Q-Learning
Authors: Zahmatkesh Mohsen , Emami Seyyed , Banazadeh Afshin , Castaldi Paolo
Published: December 2022

Attitude control of a novel regional truss-braced wing (TBW) aircraft with low stability characteristics is addressed in this paper using Reinforcement Learning (RL). In recent years, RL has been increasingly employed in challenging applications, particularly, autonomous flight control. However, a significant predicament confronting discrete RL algorithms is the dimension limitation of the state-action table and difficulties in defining the elements of the RL environment. To address these issues, in this paper, a detailed mathematical model of the mentioned aircraft is first developed to shape an RL environment. Subsequently, Q-learning, the most prevalent discrete RL algorithm, will be implemented in both the Markov Decision Process (MDP) and Partially Observable Markov Decision Process (POMDP) frameworks to control the longitudinal mode of the proposed aircraft. In order to eliminate residual fluctuations that are a consequence of discrete action selection, and simultaneously track variable pitch angles, a Fuzzy Action Assignment (FAA) method is proposed to generate continuous control commands using the trained optimal Q-table. Accordingly, it will be proved that by defining a comprehensive reward function based on dynamic behavior considerations, along with observing all crucial states (equivalent to satisfying the Markov Property), the air vehicle would be capable of tracking the desired attitude in the presence of different uncertain dynamics including measurement noises, atmospheric disturbances, actuator faults, and model uncertainties where the performance of the introduced control system surpasses a well-tuned Proportional–Integral–Derivative (PID) controller.
Static and Dynamic Analysis of Re-Entry Vehicle Nose Structures Made of Different Functionally Graded Materials
Authors: Balaraman Panneerselvam , Stephen Joseph Raj Vijayaraj , Sreehari Veloorillom Madhavan
Published: December 2022

High-speed aerospace applications, such as re-entry vehicles, mostly involve thin-walled structural components with a high strength-to-weight ratio and high-temperature resistant. The present novel work comprises the structural and thermal analysis of re-entry vehicle nose structures made of four functionally graded materials (FGM). Four FGM shell structures made of aluminum/silicon carbide, aluminum/aluminum oxide, Ti-6Al-4V/silicon carbide and Ti-6Al-4V/aluminum oxide have been considered for the re-entry vehicle nose. The effect of various thermal environments, as well as the linear temperature rise from metal-rich to ceramic-rich on critical buckling temperature and natural frequency have been studied. The critical buckling temperature, as well as the natural frequency of the large, thin re-entry vehicle nose structures, decrease with an increase in a uniform thermal environment, as well as linear temperature rise. The effect of shell thickness on buckling and dynamic characteristics of an FGM shell is also studied, suiting the nose of the re-entry vehicle under various linear temperature rises. The critical buckling temperature and natural frequency are quantified for several cases, and it was observed that they are significantly influenced by the shell thickness. Thus, the research intends to determine the thickness required for such thin and large shells to withstand in the re-entry thermal conditions.
Mars Space Exploration and Astronautical Religion in Human Research History: Psychological Countermeasures of Long-Term Astronauts
Authors: Kim David W.
Published: December 2022

As the development of science and technology has reached the point where the desire to travel to Mars has become a tangible reality, the physical limits of human movement are also part of the systematic research based on the space environment. The critical issues of radiation, altered gravity, hostile environment, isolation or confinement, and distance from Earth (travel time) are the five major hazards for astronauts during spaceflight. The prepared technology of space medicine is significant for physical health. However, how would the lone space exploration (2.5 to three years) affect the mental conditions of the astronauts? How can the space community keep astronauts safe from psychological obstacles, such as depression, conflict, resentment, bipolar disorder, obsession, and addiction? This paper explores the environmental factors of a healthy lifestyle (well-being) of the spacecraft. It presumes that a successful mission often relies on positive interactions between crew members and between the crew and ground personnel. The paper considers the mental sustainability from stress, emotions, and perceptions to improve human tonicity or vitality and argues a new mental strategy in space exploration policy that the role of an astronautical religion beyond human intelligence and artificial intelligence (AI) could be a psychiatric anchor (in a moral, ethical, and self-sacrificial context) of each astronaut and leadership of the space team as a psychoanalytical countermeasure, along with physical exercise, hobbies, pets, and virtual and augmented reality (VR/AR) entertainment, especially in the case of unexpected crises where science and technology fail its general function.
How the Composition of Substrates for Seedling Production Affects Earthworm Behavior
Authors: Ečimović Sandra , Velki Mirna , Mikuška Alma , Bažon Jelena , Kovačić Lucija Sara , Kristek Suzana , Jović Jurica , Nemet Franjo , Perić Katarina , Lončarić Zdenko
Published: December 2022

The constant increase in the intensity of agricultural production simultaneously increases the risk of negative effects of long-term agricultural practices. By-products of agricultural, forestry, and food production, as well as other types of organic waste, can be used as raw materials in the production of organic fertilizers and substrates for seedling cultivation through various processes of biological stabilization. In this way, the amount of waste is reduced, which contributes to the preservation of soil fertility and the sustainable use of resources. During waste processing and the stabilization of organic matter can be improved by using earthworms (vermicomposting). The aim of this study was to determine how different substrates, composed of different components and their mixtures, affect the earthworm Eisenia andrei. The effects of investigated substrates on the survival and behavior of earthworms were monitored. In addition, the effect of tested substrates on acetylcholinesterase (AChE), carboxylesterase (CES), and glutathione S-transferase (GST) activity was also assessed. The results showed that the most suitable substrates were leaves with horse manure and grape pomace alone and in combination with rock wool and sawdust. The obtained results provide important information on components and mixtures that have the greatest potential in the production of organic fertilizers and substrates for growing seedlings.
Important Policy Parameters for the Development of Inclusive Digital Agriculture: Implications for the Redistributive Land Reform Program in South Africa
Authors: Mazwane Sukoluhle , Makhura Moraka N. , Senyolo Mmapatla P.
Published: December 2022

The creation and deployment of digital technologies throughout the agro-food system contribute to achieving Sustainable Development Goal nine. However, various digitalization studies offer limited insight into the policy issues and solutions around emerging technologies and big data systems in agriculture. There is a need for an additional understanding of how agricultural policymaking should respond to the usage of digital technologies in the agri-food sector. Furthermore, evidence is limited on how existing agricultural government programs such as land reform can be linked with digitalization policy. This systematic review of literature sought to determine the transformation that is needed in the political and economic environment for the digital revolution to take place within South African land reform agriculture. A thematic analysis of data sampled from ProQuest Central, Scopus, Dimensions, and Google Scholar reveals five areas of intervention from agricultural policymaking. The digital revolution in agriculture can be brought about by transforming digital infrastructure, data interoperability and governance, digital markets, the compatibility of government incentives with the private sector, and the digital cultural landscape. This essay contributes to agricultural policy and decision-making dialogues that pay attention to digital technologies and land reform programs in South Africa.
Experimental Study on Water Distribution and Droplet Kinetic Energy Intensity from Non-Circular Nozzles with Different Aspect Ratios
Authors: Wang Zixin , Jiang Yue , Liu Jialing , Li Hong , Li Hao
Published: December 2022

(1) Background: In sprinkler irrigation systems, the water distribution and droplet kinetic energy are affected by the shape of the nozzle. In this paper, the effects of working pressure and aspect ratio (L/D) of circular and non-circular nozzles (diamond and ellipse) on water distribution and droplet kinetic energy intensity were investigated; (2) Methods: The hydraulic performance of a PY15 impact sprinkler with circular and non-circular nozzles was assessed under different working pressures, and the droplet diameter, velocity, and kinetic energy intensity were measured by a 2D video disdrometer. Moreover, the coefficient of variation (CV) and form factor (β) were introduced to represent the water distribution and droplet characteristics; (3) Results: The results revealed that, under the same working pressure, the CV of the diamond nozzle was the smallest compared with that of the circular and elliptical nozzles, reflecting a more uniform water distribution. The uniformity of water distribution was the best when the L/D of the elliptical nozzle was the smallest. In general, the larger the outlet diameter, the larger the wetted radius and water application rate. In addition, the smaller the L/D, the smaller the peak water distribution value and the radial increase of the kinetic energy intensity of a single nozzle. The maximum droplet kinetic energy per unit volume of the elliptical nozzle was the smallest compared with that of the circular and diamond nozzles. The circular nozzle at 200 kPa and the diamond and elliptical nozzles at 100 kPa obtained the highest uniformity coefficients of combined kinetic energy intensity distribution, which were 55.93% (circular), 67.59% (diamond), and 57.78% (elliptical) when the combination spacings were 1.0 R, 1.1 R and 1.2 R, and 1.0 R, respectively. Finally, the fitting function of unit volume droplet kinetic energy, distance from the nozzle, L/D, and working pressure of non-circular nozzles was established, and a fitting coefficient of 0.92 was obtained, indicating that the fitting equation was accurate; (4) Conclusions: At low working pressures, the elliptic and diamond nozzles showed better water distributions than the circular nozzle. The distal average droplet diameters of the sprinkler with non-circular nozzles were found to be smaller than those produced by the circular nozzle.
Aromatic Plants Metabolic Engineering: A Review
Authors: Shelepova Olga V. , Baranova Ekaterina N. , Tkacheva Ekaterina V. , Evdokimenkova Yulia B. , Ivanovskii Aleksandr A. , Konovalova Ludmila N. , Gulevich Alexander A.
Published: December 2022

Secondary metabolites of aromatic plants are used in many health applications as drugs, pheromones, insecticides, fragrances, and antioxidants. Due to the huge commercial demand for these secondary metabolites, the need to overcome the insufficient productivity of aromatic plants has become a significant challenge. Plant breeding is a traditional, labor-intensive, and limited method to improve the ability of aromatic plants to produce secondary metabolites. Modern methods of biotechnology, including genetic engineering and genome editing, can be useful and cost-effective in improving aromatic plants, as they can increase the efficiency of obtaining plants with high productivity and the creation of resistant forms and breeding lines. This review illustrates the importance of developing methods for the modification of aromatic plants belonging to different families, with a predictable quality, resistance to adverse factors and pests, and intensive growth and high yields and productivity of valuable essential oils. Particular attention is paid to successful examples of the modification of aromatic plants, applied methods, and principal approaches
Effect of Carbohydrate Nutrition on Egg Load and Population Parameters of Four Trichogramma Species
Authors: Wang Yong , Iqbal Asim , Mu Ming-Yue , Zang Zhuo-Yi , Hou Yang-Yang , Zang Lian-Sheng
Published: December 2022

Trichogramma parasitoids are the most widely used biocontrol agents and play a crucial role in the biological control of agricultural and forest pests. Many studies have shown that Trichogramma parasitoids feeding on carbohydrate rich diet can effectively improve their egg laying capacity and longevity. However, few studies have reported the impact of carbohydrate-rich diet on the biological parameters of different species of Trichogramma parasitoids. In this study, we compared the differences between the biological parameters of four Trichogramma species (T. dendrolimi, T. chilonis, T. leucaniae and T. ostriniae) before and after feeding on carbohydrate-rich diet. The results showed that all the biological parameters of Trichogramma species improved after feed on carbohydrate-rich diet. After eclosion, T. dendrolimi had the highest egg load (51.89 eggs) followed by T. chilonis (26.86 eggs), T. ostriniae (24.71 eggs), and T. leucaniae (12.44 eggs). Trichogramma dendrolimi showed an increased trend in egg load (66.7–86.21 eggs) after feeding on carbohydrate-rich diet, followed by T. chilonis (33.12–45.56 eggs), as compared to the other three species. Furthermore, T. leucaniae and T. ostriniae benefited much less from the ingestion of carbohydrate-rich diet, as only accelerated egg maturation in T. ostriniae was observed. Most of the egg loads consisted of matured eggs, so the trend of mature eggs was the same as that of the egg load. We also tested the population parameters of the Trichogramma species using the TWOSEX-MSChart. The results showed that the Trichogramma species fed with a honey diet had higher fecundities, i.e., T. dendrolimi (146.64), T. chilonis (173.53), T. leucaniae (83.69), and T. ostriniae (80.45), than when fed with water, i.e., 78.49, 40.15, 32.45 and 35.47, respectively. T. dendrolimi and T. chilonis had a higher intrinsic rate of increase r (0.4178 d−1, 0.3933 d−1), finite rate of increase λ (1.5186 d−1, 1.4818 d−1), and net reproductive rate R0 (130 offspring, 147.4 offspring), than T. leucaniae and T. ostriniae (r = 0.3373 d−1, 0.3317 d−1; λ = 1.4011 d−1, 1.3934 d−1; R0 = 73.2 offspring, 68.1 offspring) after feeding on a honey diet. We conclude that carbohydrate-rich diet significantly enhanced the biological control efficacy of Trichogramma, but the performance differed among different species. The results of this study contribute to improving the efficient application of Trichogramma parasitoids in the field.
Rhizobium Inoculation Improved the Rhizosphere P Dynamics and P Uptake Capacity of Pigeon Pea Plants Grown in Strongly Weathered Soil Only under P Fertilized Conditions
Authors: Yamamoto Saki , Okazaki Shin , Monica Nakei D. , Ohkama-Ohtsu Naoko , Tanaka Haruo , Sugihara Soh
Published: December 2022

The improvement of phosphorus (P) use efficiency (PUE) is a critical problem in crop production because of phosphorus’ scarcity. Especially in strongly weathered soil with a high P fixation capacity, a low PUE generally limits plant growth. Here, in a 70-day pigeon pea cultivation pot experiment using Ultisols, we evaluated the effects of Rhizobium inoculation (−I/+I) on the plant growth, rhizosphere, bulk soil P dynamics, and plant root P acquisition characteristics, with or without P fertilization (0P: no P application; 50SSP:50 kg P ha−1 with single superphosphate). The combination of Rhizobium inoculation with P fertilization (50SSP + I) increased the plant growth, P uptake, and organic acid content per pot by 63%, 41%, and 130%, respectively, but not without P fertilization (0P + I). The labile and moderately labile inorganic P (NaHCO3-Pi and NaOH-Pi) contents were higher (55% and 44%, respectively) in the rhizosphere soil than those in the bulk soil in the 50SSP + I treatment, indicating the efficient solubilization of the applied P under the 50SSP + I treatment. The fertilized PUE was higher in the 50SSP + I treatment (26%) than that in the 50SSP−I treatment (15%). Thus, these results suggest that Rhizobium inoculation with 50SSP should stimulate plant root P acquisition characteristics, leading to the solubilization of applied P in the rhizosphere and efficient plant P uptake. In conclusion, the 50SSP + I treatment effectively improved the PUE of pigeon peas in strongly weathered soil.