bluuebunny/crossref_metadata_2025 huggingface dataset:
Communicative Approach in Teaching Foreign Languages
Authors: Allanazarova M.
Published: November 2021

The Communitive Approach in language teaching starts from а theory of language as communication. The goal of language teaching is to develop communicative competence. Another linguistic theory of communication favored in CLT is functional account of language use. Linguistic is concerned with the description of speech acts of texts, since only through study of language in use are all the functions of language and therefore all components of meaning brought into focus. The goal of language teaching is to develop what referred to as “communicative competence”. This term is coined in order to contrast а communicative view of language and theory of competence. Linguistic theory is concerned primarily with an ideal speaker-listener in а completely homogeneous speech community, who knows its language perfectly and is unaffected by such grammatically irrelevant conditions as memory limitation, distribution, shifts of attention and interest, and errors (random or characteristic) in applying his knowledge of the language in actual performance.
Peculiarities of the Activities of the Physical Education Teacher and the Trainer
Authors: Rzayev O.
Published: November 2021

Teaching and upbringing in physical education lessons is a complex multifaceted process, the effectiveness of which depends not only on what the teacher himself knows and can do, but also on how he transfers knowledge and skills to students. Pedagogical activity is a continuous solution of pedagogical problems. A feature of the conditions for the activity of a physical education teacher is the need to show physical exercises and ensure students when they perform physical exercises, as well as move with students when exercising in the air, on hikes, etc. The indicator of the coach's efficiency is the successful achievement of the goal with the most rational use of forces and means. In other words, efficiency presupposes the correspondence of the structure and functioning of the trainer's psyche to the structure and dynamics of his activity.
Translating Methods of Realities in English, Russian and Kyrgyz Culture
Authors: Karabekova E. , Abdykadyrova S.
Published: November 2021

This article is devoted to the problem of translation of linguistic realities in English, Russian and Kyrgyz cultures. The article is an overview of the realities in the mentioned cultures. An attempt is made to find ways to overcome the linguo-ethnic barrier when translating concepts from the Kyrgyz language, reflecting the national character. The essence of this problem boils down to the fact that the main requirement of translation is to preserve the originality of the translated text and to study the cultural component of the text. It should be aimed at identifying the differences between the original and the translation, not only because of the linguistic form, but also because of the cultural one. The main purpose of the article is to analyze the elements of the linguistic specificity of translation of realities in English, Russian and Kyrgyz cultures. The subject of the research is ethnic elements — objective problems arising during translation, and methods of their solution, that is, the transfer of cultural realities during translation. The article examines the theory of cultural realities in the translation aspect and analyzes the main features and methods of translating words-realities. The selected realities are analyzed from the point of view of their translation methods to identify the most effective ones. To solve the set specific tasks, the following methods were used in the work: analysis, synthesis, classification and generalization of the data obtained. As a result of the study, material was obtained, the analysis of which made it possible to conclude that the choice of one way or another way to overcome the linguo-ethnic barrier depends on the situation of intercultural communication, as well as on the goals of the participants in the communication.
Deglutition Disorders from the Perspective of Healthcare Professionals in Turkey
Authors: SEREL ARSLAN Selen , DEMIR Numan , KARADUMAN Aynur Ayşe
Published: November 2021

Objective: Awareness of deglutition disorders is essential for prevention of deglutition-related complications and improving quality of care.The study examined the current knowledge, attitude and practice regarding deglutition disorders among healthcare professionals in Turkey.Methods: Healthcare professionals who worked in hospitals and special education schools were included. A survey examining knowledge,attitudes, and practices regarding deglutition disorders, originally designed by Farpour and colleagues (2019), was adapted and administered tohealthcare professionals working in hospitals and special education schools in Turkey.Results: A total of 270 healthcare professionals were responded (72.7% response rate). 254 participants (94.1%) were familiar with theterm deglutition disorders. 223 participants (82.6%) defined their professions as a management team member. All participants supporteda multidisciplinary approach. 216 (80%) participants met a patient with deglutition disorders. 212 participants (78.5%) used at least oneevaluation method to evaluate swallowing function, and 147 participants (54.5%) used at least one treatment method for treatment. Mostparticipants (73.7%) were interested in attending a workshop about deglutition disorders.Conclusion: The present study showed that knowledge about deglutition disorders is high among healthcare professionals in Turkey, and theybelieve the necessity of multidisciplinary approach in deglutition disorders. However, it is not understood if they follow an individualized andstandardized evaluation and management procedures. Therefore, more detailed questionnaires especially focus on evaluation and managementof deglutition disorders should be developed and healthcare professionals could be trained to improve management of deglutition disordersin Turkey.
An ECHO of Cartilage: In Silico Prediction of Combinatorial Treatments to Switch Between Transient and Permanent Cartilage Phenotypes With Ex Vivo Validation
Authors: Khurana Sakshi , Schivo Stefano , Plass Jacqueline R. M. , Mersinis Nikolas , Scholma Jetse , Kerkhofs Johan , Zhong Leilei , van de Pol Jaco , Langerak Rom , Geris Liesbet , Karperien Marcel , Post Janine N.
Published: November 2021

A fundamental question in cartilage biology is: what determines the switch between permanent cartilage found in the articular joints and transient hypertrophic cartilage that functions as a template for bone? This switch is observed both in a subset of OA patients that develop osteophytes, as well as in cell-based tissue engineering strategies for joint repair. A thorough understanding of the mechanisms regulating cell fate provides opportunities for treatment of cartilage disease and tissue engineering strategies. The objective of this study was to understand the mechanisms that regulate the switch between permanent and transient cartilage using a computational model of chondrocytes, ECHO. To investigate large signaling networks that regulate cell fate decisions, we developed the software tool ANIMO, Analysis of Networks with interactive Modeling. In ANIMO, we generated an activity network integrating 7 signal transduction pathways resulting in a network containing over 50 proteins with 200 interactions. We called this model ECHO, for executable chondrocyte. Previously, we showed that ECHO could be used to characterize mechanisms of cell fate decisions. ECHO was first developed based on a Boolean model of growth plate. Here, we show how the growth plate Boolean model was translated to ANIMO and how we adapted the topology and parameters to generate an articular cartilage model. In ANIMO, many combinations of overactivation/knockout were tested that result in a switch between permanent cartilage (SOX9+) and transient, hypertrophic cartilage (RUNX2+). We used model checking to prioritize combination treatments for wet-lab validation. Three combinatorial treatments were chosen and tested on metatarsals from 1-day old rat pups that were treated for 6 days. We found that a combination of IGF1 with inhibition of ERK1/2 had a positive effect on cartilage formation and growth, whereas activation of DLX5 combined with inhibition of PKA had a negative effect on cartilage formation and growth and resulted in increased cartilage hypertrophy. We show that our model describes cartilage formation, and that model checking can aid in choosing and prioritizing combinatorial treatments that interfere with normal cartilage development. Here we show that combinatorial treatments induce changes in the zonal distribution of cartilage, indication possible switches in cell fate. This indicates that simulations in ECHO aid in describing pathologies in which switches between cell fates are observed, such as OA.
Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through GCN5 and Wnt/β-Catenin
Authors: Liu Yanchang , Cheng Wendan , Zhao Yao , Gao Liang , Chang Yongyun , Tong Zhicheng , Li Huiwu , Jing Juehua
Published: November 2021

Bone marrow mesenchymal stem cells (BMSCs) play a critical role in bone formation and are extremely sensitive to external mechanical stimuli. Mechanical signals can regulate the biological behavior of cells on the surface of titanium-related prostheses and inducing osteogenic differentiation of BMSCs, which provides the integration of host bone and prosthesis benefits. But the mechanism is still unclear. In this study, BMSCs planted on the surface of TiO2 nanotubes were subjected to cyclic mechanical stress, and the related mechanisms were explored. The results of alkaline phosphatase staining, real-time PCR, and Western blot showed that cyclic mechanical stress can regulate the expression level of osteogenic differentiation markers in BMSCs on the surface of TiO2 nanotubes through Wnt/β-catenin. As an important member of the histone acetyltransferase family, GCN5 exerted regulatory effects on receiving mechanical signals. The results of the ChIP assay indicated that GCN5 could activate the Wnt promoter region. Hence, we concluded that the osteogenic differentiation ability of BMSCs on the surface of TiO2 nanotubes was enhanced under the stimulation of cyclic mechanical stress, and GCN5 mediated this process through Wnt/β-catenin.
A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application
Authors: Lu Nan , Zhang Chenglin , Zhang Wenjie , Xu Haoran , Li Yuhong , Wei Minhua , Meng Jing , Meng Yan , Wang Junzhe , Chen Ning
Published: November 2021

Corynebacterium glutamicum is one of the important industrial microorganisms for production of amino acids and other value-added compounds. Most expression vectors used in C. glutamicum are based on inducible promoter (Ptac or Ptrc) activated by isopropyl-β-D-thiogalactopyranoside (IPTG). However, these vectors seem unsuitable for large-scale industrial production due to the high cost and toxicity of IPTG. Myo-inositol is an ideal inducer because of its non-toxicity and lower price. In this study, a myo-inositol-inducible expression vector pMI-4, derived from the expression vector pXMJ19, was constructed. Besides the original chloramphenicol resistance gene cat, multiple cloning sites, and rrnB terminator, the pMI-4 (6,643 bp) contains the iolRq cassette and the myo-inositol-inducible promoter PiolT1. The pMI-4 could stably replicate in the C. glutamicum host. Meanwhile, the non-myo-inositol degradation host strain C. glutamicumΔiolGΔoxiCΔoxiDΔoxiE for maintaining the pMI-4 was developed. Overexpression of hemAM and hemL using pMI-4 resulted in a significant accumulation of 5-aminolevulinic acid, indicating its potential application in metabolic engineering and industrial fermentation.
Reducing Inflammation and Vascular Invasion in Intervertebral Disc Degeneration via Cystathionine-γ-Lyase Inhibitory Effect on E-Selectin
Authors: Xu Haoran , Wei Kang , Tu Jingyao , Chen Yangmengfan , He Yi , Ding Yifan , Xu Huanhuan , Bao Xinyu , Xie Hui , Fang Huang , Wang Huan
Published: November 2021

The incidence of degenerative spinal diseases, such as cervical spondylosis and thoracic and lumbar disc herniation, is increasing. These health problems have adversely affected human life and work. Surgical intervention is effective when intervertebral disc degeneration (IDD) causes nerve compression and/or severely limits daily activity. Early IDD patients generally do not require surgery. However, there is no effective method of impeding IDD progression. Thus, novel approaches to alleviating IDD deterioration are urgently required. Cystathionine-γ-lyase (CSE) and E-selectin (CD62E) are vital factors regulating vascular function and inflammation. However, their effects on IDD and vascular invasion in intervertebral discs (IVDs) are pending further exploration. Here, bioinformatics and human nucleus pulposus (NP) tissues analyses revealed that CSE was significantly downregulated and CD62E was upregulated in the NP tissues of IDD patients. We demonstrated that CSE overexpression, CD62E downregulation, and NF-κB (P65) inhibition mitigate inflammation and recover metabolic function in NP cells. Similarly, CSE attenuated vascular invasion induced by inflammatory irritation. Using a rat IDD model, we showed that CSE improved degeneration, inflammation, and microvascular invasion in NP tissue, whereas CD62E had the opposite effect. Taken together, our results indicated that the CSE/CD62E pathway could effectively improve the inflammatory environment and vascular invasion in IVD. Hence, the findings of this study propose a promising and valuable strategy for the treatment of patients with early IDD as well as postoperative adjuvant therapy in patients with severe IDD.
Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster
Authors: Sanchez-Mirasierra Irene , Hernandez-Diaz Sergio , Ghimire Saurav , Montecinos-Oliva Carla , Soukup Sandra-Fausia
Published: November 2021

Automatic quantification of image parameters is a powerful and necessary tool to explore and analyze crucial cell biological processes. This article describes two ImageJ/Fiji automated macros to approach the analysis of synaptic autophagy and exosome release from 2D confocal images. Emerging studies point out that exosome biogenesis and autophagy share molecular and organelle components. Indeed, the crosstalk between these two processes may be relevant for brain physiology, neuronal development, and the onset/progression of neurodegenerative disorders. In this context, we describe here the macros “Autophagoquant” and “Exoquant” to assess the quantification of autophagosomes and exosomes at the neuronal presynapse of the Neuromuscular Junction (NMJ) in Drosophila melanogaster using confocal microscopy images. The Drosophila NMJ is a valuable model for the study of synapse biology, autophagy, and exosome release. By use of Autophagoquant and Exoquant, researchers can have an unbiased, standardized, and rapid tool to analyze autophagy and exosomal release in Drosophila NMJ.

Code available at: https://github.com/IreneSaMi/Exoquant-Autophagoquant
Design, Synthesis, and Biological Evaluation of Pyrano[2,3-c]-pyrazole–Based RalA Inhibitors Against Hepatocellular Carcinoma
Authors: Wang Yuting , He Mingyao , Li Xiang , Chai Jinlong , Jiang Qinglin , Peng Cheng , He Gu , Huang Wei
Published: November 2021

The activation of Ras small GTPases, including RalA and RalB, plays an important role in carcinogenesis, tumor progress, and metastasis. In the current study, we report the discovery of a series of 6-sulfonylamide-pyrano [2,3-c]-pyrazole derivatives as novel RalA inhibitors. ELISA-based biochemical assay results indicated that compounds 4k–4r suppressed RalA/B binding capacities to their substrates. Cellular proliferation assays indicated that these RalA inhibitors potently inhibited the proliferation of HCC cell lines, including HepG2, SMMC-7721, Hep3B, and Huh-7 cells. Among the evaluated compounds, 4p displayed good inhibitory capacities on RalA (IC50 = 0.22 μM) and HepG2 cells (IC50 = 2.28 μM). Overall, our results suggested that a novel small-molecule RalA inhibitor with a 6-sulfonylamide-pyrano [2, 3-c]-pyrazole scaffold suppressed autophagy and cell proliferation in hepatocellular carcinoma, and that it has potential for HCC-targeted therapy.
Unveiling the Enzymatic Degradation Process of Biobased Thiophene Polyesters
Authors: Bertolini Federico A. , Soccio Michelina , Weinberger Simone , Guidotti Giulia , Gazzano Massimo , Guebitz Georg M. , Lotti Nadia , Pellis Alessandro
Published: November 2021

In the past 20 years, scientific research focused on the identification of valid alternatives to materials of fossil origin, in particular, related to biobased polymers. Recently, the efforts led to the synthesis of thiophene-based polymers (TBPs), a new class of polyesters based on 2,5-thiophenedicarboxylic acid (TPCA) that can be industrially produced using biomass-derived molecules. In this study, TBPs were synthesized using diols with different chain length (from C4 to C6) leading to poly(butylene 2,5-thiophenedicarboxylate) (PBTF), poly(pentamethylene 2,5-thiophenedicarboxylate) (PPeTF), and poly(hexamethylene 2,5-thiophenedicarboxylate) (PHTF), respectively, that were processed to thin films. To investigate enzymatic hydrolysis of these polymer films, cutinase 1 (Thc_cut1) and cutinase 2 (Thc_cut2) from Thermobifida cellulosilytica were recombinantly expressed in the host E. coli and purified. After 72 h of incubation at 65°C with 5 µM Thc_cut1, weight loss and HPLC analysis indicated 9, 100, and 80% degradation of PBTF, PPeTF, and PHTG with a concomitant release of 0.12, 2.70, and 0.67 mM of TPCA. The SEM analysis showed that tiny holes were formed on the surface of the films and after 72 h PPeTF was completely degraded. The LC-TOF/MS analysis indicated that Thc_cut2 in particular released various oligomers from the polymer during the reaction. In addition, the FTIR analysis showed the formation of novel acid and hydroxyl groups on the polymer surfaces. The results showed that the two used thermostable cutinases are promising biocatalysts for the environmentally friendly degradation of TPCA-based polyesters, in view of a possible sustainable recycling of plastic waste through resynthesis processes.
Influence of Direct Current–Voltage Accompanied by Charge Flow on CO2 Hydrate Formation
Authors: Zhao Qi , Xia Zhi-Ming , Xu Chun-Gang , Chen Zhao-Yang , Li Xiao-Sen
Published: November 2021

The capture and storage of carbon dioxide (CO2) are urgent and crucial to achieve the goal of carbon neutrality. Hydrate-based CO2 capture technology is one of the promising technologies for capturing and storing CO2. This work studied the nucleation and growth of CO2 hydrate provoked by direct current–voltage accompanied by charge flow with the agitation of 450 rpm at an initial pressure of 3.5 MPa and a temperature of 274.15 K. The results show that the physical bubble behavior and electrochemistry mechanisms could influence CO2 hydrate formation process in the application of voltage. The induction time and semi-completion time of CO2 hydrate formation were decreased by 51% and 27.8% in the presence of 15 V, respectively. However, more product of electrolysis, Joule heat and ions, could inhibit the CO2 hydrate formation process in the application of a high voltage (60 V). In addition, a high voltage (60 V) could change the morphology characteristics of CO2 hydrate from gel-like to whisker-like. This study provides valuable information on the formation of CO2 hydrate under the action of charge flow.
Elevational Diversity Patterns of Green Lacewings (Neuroptera: Chrysopidae) Uncovered With DNA Barcoding in a Biodiversity Hotspot of Southwest China
Authors: Lai Yan , Liu Yunhui , Liu Xingyue
Published: November 2021

Elevational diversity patterns can reflect the responses of biodiversity to climate change spatially. We investigate the species diversity patterns of green lacewings (an important predatory group of insects) along the gradient of elevation from the Shaluli Mountains (Mts. Shaluli), which belong to the Hengduan Mountains in southwestern China, one of the important hotspots of global biodiversity. We combined multiple approaches, including Automatic Barcode Gap Discovery (ABGD), Assemble Species by Automatic Partitioning analysis (ASAP), General Mixed Yule Coalescent (GMYC), Poisson tree processes (bPTP), multi-rate Poisson tree processes (mPTP), to delimit the green lacewings species based on the standard barcoding region of cytochrome c oxidase subunit I (COI). The α-diversity and β-diversity patterns of green lacewings from the Mts. Shaluli along the gradient of elevation were analyzed, with further exploration on how the temperature effect elevational-diversity pattern on broad-scale (county scale) elevational gradients. The DNA barcoding reference library consisted of 40 green lacewing species from the Mts. Shaluli. The α-diversity of green lacewings decreased with the increasing elevation. The temperature was found to have a significant effect on the abundance and Shannon-Wiener diversity index but not on the species richness. Nestedness replaced turnover as the main component of Sørensen’s dissimilarity with the increasing elevation, and greater nestedness occurred at low temperature areas. The combination of a reliable DNA barcoding database could improve the accuracy and efficiency to investigate the species diversity patterns of green lacewings. Temperature, resource, and resultant interspecific competitions may have important roles in explaining the species diversity patterns of green lacewings from the Mts. Shaluli. Priority of conservation should be given to the species at low elevation, middle elevation, and relatively high temperature regions under the background of global climate warming.
Phenylalanine 4-Hydroxylase Contributes to Endophytic Bacterium Pseudomonas fluorescens’ Melatonin Biosynthesis
Authors: Jiao Jian , Xia Yan , Zhang Yingli , Wu Xueli , Liu Chonghuai , Feng Jiancan , Zheng Xianbo , Song Shangwei , Bai Tuanhui , Song Chunhui , Wang Miaomiao , Pang Hongguang
Published: November 2021

Melatonin acts both as an antioxidant and as a growth regulatory substance in plants. Pseudomonas fluorescens endophytic bacterium has been shown to produce melatonin and increase plant resistance to abiotic stressors through increasing endogenous melatonin. However, in bacteria, genes are still not known to be melatonin-related. Here, we reported that the bacterial phenylalanine 4-hydroxylase (PAH) may be involved in the 5-hydroxytryptophan (5-HTP) biosynthesis and further influenced the subsequent production of melatonin in P. fluorescens. The purified PAH protein of P. fluorescens not only hydroxylated phenylalanine but also exhibited l-tryptophan (l-Trp) hydroxylase activity by converting l-Trp to 5-HTP in vitro. However, bacterial PAH displayed lower activity and affinity for l-Trp than l-phenylalanine. Notably, the PAH deletion of P. fluorescens blocked melatonin production by causing a significant decline in 5-HTP levels and thus decreased the resistance to abiotic stress. Overall, this study revealed a possible role for bacterial PAH in controlling 5-HTP and melatonin biosynthesis in bacteria, and expanded the current knowledge of melatonin production in microorganisms.
Electromagnetic Response of Clustered Charged Particles
Authors: Ye Wenzheng , Hu Xiaofeng , Zhou Shuai , Wang Chi , Jiang Jing , Yang Ting , Gao Fei
Published: November 2021

Electromagnetic response of clustered charged particles is the foundation of electromagnetic wave interaction with various natural phenomena, such as sandstorm, cloud, and volcano eruption. Previous studies usually employed assumption of independent charged particles, without considering the coupling between them. Here, we build up a general numerical model considering the multiple scattering effect, and test it with a charged two- and four-particle system. The numerical results show that independence assumption fails, while the number density of clustered charged particles is getting larger. This work may pave the way for deeper understanding on the electromagnetic interaction of clustered charged particles.
Role of BMI in the Relationship Between Dietary Inflammatory Index and Depression: An Intermediary Analysis
Authors: Ma Yuxia , Li Ruiqiang , Zhan Wenqiang , Huang Xin , Zhang Zechen , Lv Shuaishuai , Wang Jiaqi , Liang Luyao , Jia Xiaofang
Published: November 2021

Introduction: This study investigated this association and the role of BMI in the inflammatory process in a large population-based observational study.

Methods: A total of 1,865 elderly people (≥55 years) were followed from the Community Cohort Study of Nervous System Diseases (CCSNSD) cohort study from 2018 to 2019 (Mean [SD] age, 66.31 [0.32] years; 716 [38.4%] males). The semi-quantitative FFQ and geriatric depression scale (GDS) were used to evaluate the diet and depressive symptoms of the elderly, respectively. The multivariable logistic regression model estimated the OR and 95% CI between Empirical Dietary Inflammatory Index (E-DII) and depression. The interaction of E-DII and BMI on depressive events was tested, and the mediation analysis of BMI was performed.

Results: As measured by E-DII, the mean (SE) value of the inflammatory potential of the diet in our study was 1.56 (0.12). E-DII ranged from 5.23 to 5.58. In comparison with the first quartile, the elderly from the second quartile (OR: 1.15 [95% CI: 1.09, 1.28]) to the fourth quartile (OR: 1.31 [95% CI: 1.16, 1.42]) have a higher risk of depression before adjustment for BMI. An interaction was observed between E-DII and BMI in terms of the risk of depression (PInteraction < 0.001). The whole related part is mediated by BMI (31.06%).

Conclusion: Our findings indicate that the higher pro-inflammatory potential of diet is associated with a higher risk of depression, and this association may be mediated by BMI. Further research is needed to verify our findings and clarify the latent mechanism.
Secondary Prevention of Dementia: Combining Risk Factors and Scalable Screening Technology
Authors: Ojakäär Triin , Koychev Ivan
Published: November 2021

Alzheimer's disease (AD) is a progressive neurodegenerative disorder that is the most common cause of dementia. Over a third of dementia cases are estimated to be due to potentially modifiable risk factors, thus offering opportunities for both identification of those most likely to be in early disease as well as secondary prevention. Diabetes, hypertension and chronic kidney failure have all been linked to increased risk for AD and dementia and through their high prevalence are particularly apt targets for initiatives to reduce burden of AD. This can take place through targeted interventions of cardiovascular risk factors (shown to improve cognitive outcomes) or novel disease modifying treatments in people with confirmed AD pathology. The success of this approach to secondary prevention depends on the availability of inexpensive and scalable methods for detecting preclinical and prodromal dementia states. Developments in blood-based biomarkers for Alzheimer's disease are rapidly becoming a viable such method for monitoring large at-risk groups. In addition, digital technologies for remote monitoring of cognitive and behavioral changes can add clinically relevant data to further improve personalisation of prevention strategies. This review sets the scene for this approach to secondary care of dementia through a review of the evidence for cardiovascular risk factors (diabetes, hypertension and chronic kidney disease) as major risk factors for AD. We then summarize the developments in blood-based and cognitive biomarkers that allow the detection of pathological states at the earliest possible stage. We propose that at-risk cohorts should be created based on the interaction between cardiovascular and constitutional risk factors. These cohorts can then be monitored effectively using a combination of blood-based biomarkers and digital technologies. We argue that this strategy allows for both risk factor reduction-based prevention programmes as well as for optimisation of any benefits offered by current and future disease modifying treatment through rapid identification of individuals most likely to benefit from them.
High-Performance Work System, Strategic Flexibility, and Organizational Performance—The Moderating Role of Social Networks
Authors: Wang Yizhi , Cao Yi , Xi Nan , Chen Huitian
Published: November 2021

Based on the dynamic capability theory, this research investigated the effect of a high-performance work system on organizational performance, the mediating role of strategic flexibility, and the moderating role of an enterprise’s social network in this relationship. A total of 214 middle and senior managers from 58 Chinese enterprises were invited to participate in this research. The results showed that the high-performance work system is positively correlated with organizational performance and such correlation is partially mediated by strategic flexibility. Results found that the social network of an enterprise negatively moderated the relationship between a high-performance work system and strategic flexibility. However, the social network did not moderate the mediating role of strategic flexibility in high-performance work systems (HPWS) and organizational performance.
The Analysis of the Innovation Consciousness of College Student Entrepreneurs Under the Teaching Concept of Chinese Excellent Traditional Culture
Authors: Geng Bin , Huang Tianyun , Jiang Xinsheng , Lin Nana , Gao Guangyuan , Fan Lisha
Published: November 2021

The purpose of this study was to analyze the current situation of the entrepreneurial consciousness of college student entrepreneurs and to explore the role of innovative and entrepreneurial talents in social and economic development. Based on the teaching concept of Chinese excellent traditional culture, first, the relevant theories of innovation and entrepreneurship, as well as the characteristics of entrepreneurial talents in colleges and entrepreneurs, are analyzed and elaborated; moreover, the definition of college student entrepreneur is explained; then, from the perspective of entrepreneurial teaching management, entrepreneurial education, and place support, the questionnaire method is selected to show the understanding of the entrepreneurship of college students; finally, based on the Cobb-Douglas function, the model before and after the introduction of innovative and entrepreneurial talents is tested and analyzed. Investigation and analysis suggest that most college students have entrepreneurial intention, and 61.5% of them choose to start their own business after having working experience; the relative freedom of time and space is the main factor to attract college students to start their own businesses, accounting for 42.3%; 69.3% of college students think that capital is a restricting factor for entrepreneurship, while 76.2% think that lack of experience is a major restricting factor for entrepreneurship; college students have a certain demand for entrepreneurship training and guidance from the school, especially in the setting of entrepreneurship incubation park and resource pool; the characteristics of entrepreneurship, professional skills, and interpersonal resources are more crucial for college students; most college students have a positive cognition of the excellent traditional Chinese teaching concepts; the analysis based on the Cobb-Douglas function reveals that the introduction of innovative and entrepreneurial talents can promote economic development. This exploration has a positive effect on the cultivation of awareness of college students of entrepreneurship and innovation, as well as the relationship discussion between the introduction of innovative and entrepreneurial talents and social economy.
Vocabulary: Common or Basic?
Authors: Kornai András
Published: November 2021

Neither linguistics nor psychology offers a single, unified notion of simplicity, and therefore the simplest “core” layer of vocabulary is hard to define in theory and hard to pinpoint in practice. In section 1 we briefly survey the main approaches, and distinguish two that are highly relevant to lexicography: we will call these common and basic. In sections 2 and 3 we compare these approaches, and in section 4 we point the reader to Kolmogorov complexity, unfamiliar as it may be to most working psychologists, lexicographers, and educators, as the best formal means to deal with core vocabulary.
Long-Term Changes of HIV/AIDS Incidence Rate in China and the U.S. Population From 1994 to 2019: A Join-Point and Age-Period-Cohort Analysis
Authors: Ma Yudiyang , Cui Yiran , Hu Qian , Mubarik Sumaira , Yang Donghui , Jiang Yuan , Yao Yifan , Yu Chuanhua
Published: November 2021

Although HIV caused one of the worst epidemics since the late twentieth century, China and the U.S. has made substantial progress to control the spread of HIV/AIDS. However, the trends of HIV/AIDS incidence remain unclear in both countries. Therefore, this study aimed to highlight the long-term trends of HIV/AIDS incidence by gender in China and the U.S. population. The data were retrieved from the Global Burden of Disease (GBD) database since it would be helpful to assess the impact/role of designed policies in the control of HIV/AIDS incidence in both countries. The age-period-cohort (APC) model and join-point regression analysis were employed to estimate the age-period-cohort effect and the average annual percentage change (AAPC) on HIV incidence. Between 1994 and 2019, we observed an oscillating trend of the age-standardized incidence rate (ASIR) in China and an increasing ASIR trend in the U.S. Despite the period effect in China declined for both genders after peaked in 2004, the age effect in China grew among the young (from 15–19 to 25–29) and the old age groups (from 65–69 to 75–79). Similarly, the cohort effect increased among those born in the early (from 1924–1928 to 1934–1938) and the latest birth groups (from 1979–1983 to 2004–2009). In the case of the U.S., the age effect declined after it peaked in the 25–29 age group. People born in recent birth groups had a higher cohort effect than those born in early groups. In both countries, women were less infected by HIV than men. Therefore, besides effective strategies and awareness essential to protect the young age groups from HIV risk factors, the Chinese government should pay attention to the elderly who lacked family support and were exposed to HIV risk factors.
A Systematic Review and Meta-Analysis Protocol of Chemoablation vs. Transurethral Resection of Bladder Tumor in Patients With Non-Muscle-Invasive Bladder Cancer
Authors: Shi Xu , Feng Dechao , Wei Wuran
Published: November 2021

Background: Bladder cancer is the second-ranked tumor of the genitourinary system. Transurethral resection of bladder tumor (TURBT) is currently the most important diagnosis and treatment method for non-muscular invasive bladder cancer (NMIBC). However, due to its high recurrence and progression rate, as well as high cost and inapplicability to some patients, intravesical chemoablation as an alternative to TURBT may be promising for NMIBC patients. However, there are very little data comparing its effectiveness, safety, best effective drug type, dosage selection, and cost with TURBT at present, which deserves further evaluation. The present study was designed in order to discuss which treatment is superior to another between chemoablation and TURBT in patients with NMIBC.

Methods and Analysis: Databases including PubMed, MEDLINE, EMBASE, and Cochrane Library databases, as well as Chinese databases including CNKI (China national knowledge infrastructure), Wan Fang database, and Chinese Clinical Trial Registry, from August 1994 to the time when the official submission of this review was published was included in this review and screened by two reviewers (XS and DCF) independently. There were no language limitations. The study was conducted according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Data was analyzed using RevMan and Stata software. The primary aims were the clinical effectiveness, including response rate, complete response OS, CSM, recurrence rate, time to recurrent, progression rate, and time to progression, among others. The secondary aims mainly included safety and tolerability, including costs, operation time, hospital stay, bleeding volume, and complications, among others.

Study Registration: This study is registered as PROSPERO CRD42021271124.
Detection of Congestive Heart Failure and Myocardial Dysfunction in Cats With Cardiomyopathy by Using Two-Dimensional Speckle-Tracking Echocardiography
Authors: Suzuki Ryohei , Saito Takahiro , Yuchi Yunosuke , Kanno Haruka , Teshima Takahiro , Matsumoto Hirotaka , Koyama Hidekazu
Published: November 2021

Congestive heart failure (CHF) is a life-threatening condition in cats with cardiomyopathy. We hypothesized that myocardial dysfunction may induce progression to CHF pathophysiology in cats with cardiomyopathy. However, no previous studies have evaluated the involvement of myocardial dysfunction in cats with CHF. In this study, we aimed to evaluate the relationship between CHF and myocardial function assessed using two-dimensional speckle-tracking echocardiography (2D-STE). Sixteen client-owned healthy cats and 32 cats with cardiomyopathy were enrolled in this study. Cats were classified into three groups: healthy cats, cardiomyopathy without CHF (CM group), and cardiomyopathy with CHF (CHF group). Left ventricular (LV) longitudinal and circumferential strains (SL and SC, respectively), and right ventricular (RV) SL were measured using 2D-STE. Logistic regression analysis was performed to assess the relationship between CHF and echocardiographic variables, including 2D-STE. Results comparing the healthy cats and CM vs. CHF groups showed that increased left atrial to aortic diameter ratio and decreased LV apical SC were significantly associated with the existence of CHF (odds ratio [95% confidence interval]: 1.40 [1.16–1.78] and 1.59 [1.06–2.36], respectively). Results comparing the CM vs. CHF group showed that increased end-diastolic RV internal dimension and decreased RV SL were significantly associated with the existence of CHF (odds ratio: 1.07 [1.00–1.13] and 1.34 [1.07–1.68], respectively). Left atrial enlargement and depressed LV apical myocardial function may be useful tools for predicting the progression to CHF in cats. Furthermore, RV enlargement and dysfunction may lead to the onset of CHF in asymptomatic cats with cardiomyopathy.
Application of Mini-Batch Metaheuristic Algorithms in Problems of Optimization of Deterministic Systems with Incomplete Information about the State Vector
Authors: Panteleev Andrei V. , Lobanov Aleksandr V.
Published: November 2021

In this paper, we consider the application of the zero-order mini-batch optimization method in the problem of finding optimal control of a pencil of trajectories of nonlinear deterministic systems in the case of incomplete information about the state vector. The pencil of trajectories originates from a given set of initial states. To solve the problem, the structure of a feedback system is proposed, which contains models of the plant, measuring system, nonlinear state observer and control law of the fixed structure with unknown coefficients. The objective function proposed considers the quality of pencil of trajectories control, which is estimated by the average value of the Bolz functional over the given set of initial states. Unknown control laws of a plant and an observer are found in the form of expansions in terms of orthonormal systems of basis functions, which are specified on the set of possible states of a dynamical system. The original pencil of trajectories control problem is reduced to a global optimization problem, which is solved using the well-proven zero-order method, which uses a modified mini-batch approach in a random search procedure with adaptation. An algorithm for solving the problem is proposed. The satellite stabilization problem with incomplete information is solved.
The Protective Effect of Ubiquinone against the Amyloid Peptide in Endothelial Cells Is Isoprenoid Chain Length-Dependent
Authors: Frontiñán-Rubio Javier , Rabanal-Ruiz Yoana , Durán-Prado Mario , Alcain Francisco Javier
Published: November 2021

Vascular brain pathology constitutes a common feature in neurodegenerative diseases that could underlie their development. Indeed, vascular dysfunction acts synergistically with neurodegenerative changes to exacerbate the cognitive impairment found in Alzheimer’s disease. Different injuries such as hypertension, high glucose, atherosclerosis associated with oxidized low-density lipoprotein or inflammation induce NADPH oxidase activation, overproduction of reactive oxygen species, and apoptosis in endothelial cells. Since it has been shown that pretreatment of cultured endothelial cells with the lipophilic antioxidant coenzyme Q10 (CoQ10) displays a protective effect against the deleterious injuries caused by different agents, this study explores the cytoprotective role of different CoQs homologues against Aβ25–35-induced damage and demonstrates that only pretreatment with CoQ10 protects endothelial brain cells from Aβ25–35-induced damage. Herein, we show that CoQ10 constitutes the most effective ubiquinone in preventing NADPH oxidase activity and reducing both reactive oxygen species generation and the increase in free cytosolic Ca2+ induced by Aβ25–35, ultimately preventing apoptosis and necrosis. The specific cytoprotective effect of CoQ with a side chain of 10 isoprenoid units could be explained by the fact that CoQ10 is the only ubiquinone that significantly reduces the entry of Aβ25–35 into the mitochondria.
Neuroprotective Properties of Food-Borne Polyphenols in Neurodegenerative Diseases
Authors: Silva Rui F. M. , Pogačnik Lea
Published: November 2021

Fruits and vegetables are the richest source of polyphenols in the regular human diet [...]
Calibration and Verification Test of Lily Bulb Simulation Parameters Based on Discrete Element Method
Authors: Dai Zhenwei , Wu Mingliang , Fang Zhichao , Qu Yongbo
Published: November 2021

In the simulation analysis of the lily harvesting process, the intrinsic parameters of the lily bulb and the contact parameters between the lily bulb and the lily mechanized harvesting equipment (Q235 steel) are deficient. Thus, the three-axis size, density, moisture content, Poisson’s ratio, elastic modulus, and other parameters of lily bulbs are measured in this paper with lily bulbs as the research object. Moreover, the discrete element model of the lily bulb was established using 3D scanning technology. The contact parameters between the lily bulb and Q235 steel were calibrated through bench test and simulation parameter test. The relative error between the measured value of the lily bulb accumulation angle and the simulated value is taken as response value to calibrate three parameters (collision recovery coefficient, static friction coefficient, and dynamic friction coefficient between lily bulbs). A regression model of the relative error of the stacking angle and three parameters is established, and the response surface is optimized. The results demonstrate that collision recovery coefficient, static friction coefficient, and dynamic friction coefficient between lily bulb and Q235 steel are 0.301, 0.423, and 0.063, respectively; these coefficients between lily bulbs are 0.455, 0.425, and 0.158, respectively. Additionally, a better combination of parameters is adopted to perform the simulation stacking test. The measured stacking angle is 32.31°, which is 0.34% in error with the stacking angle obtained by the physical stacking test. The test results suggest that the discrete element model and contact parameters of the lily bulb can be used in the discrete element simulation test. Furthermore, these research results could provide references for simulation tests, such as mechanized harvesting and post-harvest processing, of lily.
Impact of the Innate Inflammatory Response on ICU Admission and Death in Hospitalized Patients with COVID-19
Authors: Monserrat Jorge , Asunsolo Angel , Gómez-Lahoz Ana , Ortega Miguel A. , Gasalla Jose Maria , Gasulla Óscar , Fortuny-Profitós Jordi , Mazaira-Font Ferran A. , Teixidó Román Miguel , Arranz Alberto , Sanz José , Muñoz Benjamin , Arévalo-Serrano Juan , Rodríguez José Miguel , Martínez-A Carlos , Balomenos Dimitri , Álvarez-Mon Melchor
Published: November 2021

Objective: To describe the capacity of a broad spectrum of cytokines and growth factors to predict ICU admission and/or death in patients with severe COVID-19. Design: An observational, analytical, retrospective cohort study with longitudinal follow-up. Setting: Hospital Universitario Príncipe de Asturias (HUPA). Participants: 287 patients diagnosed with COVID-19 admitted to our hospital from 24 March to 8 May 2020, followed until 31 August 2020. Main outcome measures: Profiles of immune response (IR) mediators were determined using the Luminex Multiplex technique in hospitalized patients within six days of admission by examining serum levels of 62 soluble molecules classified into the three groups: adaptive IR-related cytokines (n = 19), innate inflammatory IR-related cytokines (n = 27), and growth factors (n = 16). Results: A statistically robust link with ICU admission and/or death was detected for increased serum levels of interleukin (IL)-6, IL-15, soluble (s) RAGE, IP10, MCP3, sIL1RII, IL-8, GCSF and MCSF and IL-10. The greatest prognostic value was observed for the marker combination IL-10, IL-6 and GCSF. Conclusions: When severe COVID-19 progresses to ICU admission and/or death there is a marked increase in serum levels of several cytokines and chemokines, mainly related to the patient’s inflammatory IR. Serum levels of IL-10, IL-6 and GCSF were most prognostic of the outcome measure.
Characterization of T2-Low and T2-High Asthma Phenotypes in Real-Life
Authors: Ricciardolo Fabio Luigi Massimo , Sprio Andrea Elio , Baroso Andrea , Gallo Fabio , Riccardi Elisa , Bertolini Francesca , Carriero Vitina , Arrigo Elisa , Ciprandi Giorgio
Published: November 2021

Asthma is a heterogeneous and complex condition characterized by chronic airway inflammation, which may be clinically stratified into three main phenotypes: type 2 (T2) low, T2-high allergic, and T2-high non-allergic asthma. This real-world study investigated whether phenotyping patients with asthma using non-invasive parameters could be feasible to characterize the T2-low and T2-high asthma phenotypes in clinical practice. This cross-sectional observational study involved asthmatic outpatients (n = 503) referring to the Severe Asthma Centre of the San Luigi Gonzaga University Hospital. Participants were stratified according to the patterns of T2 inflammation and atopic sensitization. Among outpatients, 98 (19.5%) patients had T2-low asthma, 127 (25.2%) T2-high non-allergic, and 278 (55.3%) had T2-high allergic phenotype. In comparison to T2-low, allergic patients were younger (OR 0.945, p < 0.001) and thinner (OR 0.913, p < 0.001), had lower smoke exposure (OR 0.975, p < 0.001) and RV/TLC% (OR 0.950, p < 0.001), higher prevalence of asthma severity grade 5 (OR 2.236, p < 0.05), more frequent rhinitis (OR 3.491, p < 0.001) and chronic rhinosinusitis with (OR 2.650, p < 0.001) or without (OR 1.919, p < 0.05) nasal polyps, but less common arterial hypertension (OR 0.331, p < 0.001). T2-high non-allergic patients had intermediate characteristics. Non-invasive phenotyping of asthmatic patients is possible in clinical practice. Identifying characteristics in the three main asthma phenotypes could pave the way for further investigations on useful biomarkers for precision medicine.