bluuebunny/crossref_metadata_2025 huggingface dataset:
Fractional Active Disturbance Rejection Positioning and Docking Control of Remotely Operated Vehicles: Analysis and Experimental Validation
Authors: Liu Weidong , Guo Liwei , Li Le , Xu Jingming , Yang Guanghao
Published: June 2024

In this paper, a fractional active disturbance rejection control (FADRC) scheme is proposed for remotely operated vehicles (ROVs) to enhance high-precision positioning and docking control in the presence of ocean current disturbances and model uncertainties. The scheme comprises a double closed-loop fractional-order PIλDμ controller (DFOPID) and a model-assisted finite-time sliding-mode extended state observer (MFSESO). Among them, DFOPID effectively compensates for non-matching disturbances, while its fractional-order term enhances the dynamic performance and steady-state accuracy of the system. MFSESO contributes to enhancing the estimation accuracy through the integration of sliding-mode technology and model information, ensuring the finite-time convergence of observation errors. Numerical simulations and pool experiments have shown that the proposed control scheme can effectively resist disturbances and successfully complete high-precision tasks in the absence of an accurate model. This underscores the independence of this control scheme on accurate model data of an operational ROV. Meanwhile, it also has the advantages of a simple structure and easy parameter tuning. The FADRC scheme presented in this paper holds practical significance and can serve as a valuable reference for applications involving ROVs.
Negotiating Gender and Kinship within Multicultural Families in Non-Highly Urbanised Areas of South Korea
Authors: Zulueta Johanna O.
Published: June 2024

This study examines the lives of marriage migrants, primarily coming from the Philippines to non-highly urbanised areas (i.e., “rural” areas) of South Korea. It looks at how these women negotiate gender norms and expectations in these multicultural families within the context of state-led multiculturalism. Semi-structured interviews with 20 Filipino marriage migrants were conducted from August to September 2023 in selected areas of Chungcheongnam-do (South Chungcheong Province) and Jeollabuk-do (North Jeolla Province). Based on the data gathered, it was found that these women have navigated gendered cultural expectations in the Korean household, thus reproducing gendered norms within the traditional Korean family and playing a significant role in keeping the family intact. However, there are also instances where these gendered expectations were subverted within these families. This study would like to interrogate whether these women are able to re-imagine a different kind of “womanhood” away from traditional family norms, thus challenging existing models of how marriage migrants are expected to perform in the context of what I call “performative multiculturalism” in ethnonationalist states such as South Korea and Japan.
LIM Zinc Finger Domain Containing 1 Risk Genotype of Recipient Is Associated with Renal Tubular Inflammation in Kidney Transplantation
Authors: Caliskan Yasar , Ozluk Yasemin , Kurashima Kento , Mirioglu Safak , Dirim Ahmet Burak , Hurdogan Ozge , Oto Ozgur Akin , Syn Marzena , Nazzal Mustafa , Jain Ajay , Edwards John , Yazici Halil , Lentine Krista L.
Published: June 2024

Background: Homozygosity for LIMS1 rs893403-GG genotype is linked to an increased risk of allograft rejection after kidney transplantation. Ischemia-reperfusion of the kidney allograft leads to long term infiltration of activated and effector-memory T lymphocytes and resulting in rejection and long-term fibrosis. However, the genotype, LIMS1 expression under ischemic conditions and the long-term histopathological relationships remain ill-defined. Methods: We examined the impact of the recipient’s LIMS1-rs893403 genotype with transplant kidney histopathology. The association of the LIMS1-rs893403 genotype and LIMS1 and GCC2 mRNA expression in ischemic donor kidneys were also examined. Recipients who underwent transplant kidney biopsy were genotyped for the LIMS1-rs893403 variant and associated deletion. Histopathological findings were compared between recipients with LIMS1 risk and non-risk genotypes. Real-time PCR and immunofluorescence staining for LIMS1 and GCC2 expression were performed in non-utilized donor kidneys. Results: Demographic, clinical, and treatment characteristics and the histopathological diagnosis were similar between recipients with rs893403 GG and AA/AG genotype. The Banff tubulitis score was higher in GG recipients (n = 24) compared to AA/AG (n = 86) recipients (1.42 ± 0.65 vs. 1.12 ± 0.66, p = 0.03). Ischemic kidneys with GG showed higher LIMS1 and GCC2 mRNA expression than kidneys with AG. Kidneys with rs893403-GG had higher tubular LIMS1 and GCC2 immunohistochemical staining compared to kidneys with rs893403-AG. Conclusions: Our data supports the role of the LIMS1 locus in kidney transplant rejection, particularly in lymphocyte infiltration into the internal aspect of the tubular basement membranes. Increased LIMS1 and GCC2 expression in ischemic donor kidneys with the GG genotype require further studies.
A Proteogenomic Approach to Unravel New Proteins Encoded in the Leishmania donovani (HU3) Genome
Authors: Adán-Jiménez Javier , Sánchez-Salvador Alejandro , Morato Esperanza , Solana Jose , Aguado Begoña , Requena Jose
Published: June 2024

The high-throughput proteomics data generated by increasingly more sensible mass spectrometers greatly contribute to our better understanding of molecular and cellular mechanisms operating in live beings. Nevertheless, proteomics analyses are based on accurate genomic and protein annotations, and some information may be lost if these resources are incomplete. Here, we show that most proteomics data may be recovered by interconnecting genomics and proteomics approaches (i.e., following a proteogenomic strategy), resulting, in turn, in an improvement of gene/protein models. In this study, we generated proteomics data from Leishmania donovani (HU3 strain) promastigotes that allowed us to detect 1908 proteins in this developmental stage on the basis of the currently annotated proteins available in public databases. However, when the proteomics data were searched against all possible open reading frames existing in the L. donovani genome, twenty new protein-coding genes could be annotated. Additionally, 43 previously annotated proteins were extended at their N-terminal ends to accommodate peptides detected in the proteomics data. Also, different post-translational modifications (phosphorylation, acetylation, methylation, among others) were found to occur in a large number of Leishmania proteins. Finally, a detailed comparative analysis of the L. donovani and Leishmania major experimental proteomes served to illustrate how inaccurate conclusions can be raised if proteomes are compared solely on the basis of the listed proteins identified in each proteome. Finally, we have created data entries (based on freely available repositories) to provide and maintain updated gene/protein models. Raw data are available via ProteomeXchange with the identifier PXD051920.
Integrating Genetic Services in the Philippine Public Health Delivery System: The Value of Networks
Authors: Padilla Carmencita D. , Abadingo Michelle E. , Maceda Ebner Bon G. , Alcausin Maria Melanie Liberty B.
Published: June 2024

The delivery of genetic services in developing countries is faced with significant challenges, despite medical and technological advances globally. The Philippines, being an archipelago, faces even more challenges, with significant disparities in access to healthcare, and tertiary medical centers and specialists being concentrated in the major cities. The utilization of different networks for the integration of genetic services in the existing public health delivery system has been valuable. Using the well-established network of the national newborn screening program, genetic services have been successfully integrated into the delivery of healthcare, even at the grassroot level. Equitable access to healthcare, including genetic services, was highlighted and supported by the enactment of the Rare Disease Law in 2016. The support of the academe to assure the sustainability of services was evident in the establishment of a genetic counseling program to augment the work of a handful of clinical geneticists. Professional societies and support groups have been instrumental in identifying genetic conditions to be prioritized and lobbying for increased public awareness, leading to national programs and policies. This paper primarily discusses the value of networks in the delivery of genetic services, specifically newborn screening, programs for rare diseases, birth defects, and genetic counseling.
A Comparative Study of Susceptibility and Hazard for Mass Movements Applying Quantitative Machine Learning Techniques—Case Study: Northern Lima Commonwealth, Peru
Authors: Badillo-Rivera Edwin , Olcese Manuel , Santiago Ramiro , Poma Teófilo , Muñoz Neftalí , Rojas-León Carlos , Chávez Teodosio , Eyzaguirre Luz , Rodríguez César , Oyanguren Fernando
Published: June 2024

This study addresses the importance of conducting mass movement susceptibility mapping and hazard assessment using quantitative techniques, including machine learning, in the Northern Lima Commonwealth (NLC). A previous exploration of the topographic variables revealed a high correlation and multicollinearity among some of them, which led to dimensionality reduction through a principal component analysis (PCA). Six susceptibility models were generated using weights of evidence, logistic regression, multilayer perceptron, support vector machine, random forest, and naive Bayes methods to produce quantitative susceptibility maps and assess the hazard associated with two scenarios: the first being El Niño phenomenon and the second being an earthquake exceeding 8.8 Mw. The main findings indicate that machine learning models exhibit excellent predictive performance for the presence and absence of mass movement events, as all models surpassed an AUC value of >0.9, with the random forest model standing out. In terms of hazard levels, in the event of an El Niño phenomenon or an earthquake exceeding 8.8 Mw, approximately 40% and 35% respectively, of the NLC area would be exposed to the highest hazard levels. The importance of integrating methodologies in mass movement susceptibility models is also emphasized; these methodologies include the correlation analysis, multicollinearity assessment, dimensionality reduction of variables, and coupling statistical models with machine learning models to improve the predictive accuracy of machine learning models. The findings of this research are expected to serve as a supportive tool for land managers in formulating effective disaster prevention and risk reduction strategies.
“I Don’t Believe in Age; I Believe in Staying Enthusiastic”: An Exploratory Qualitative Study into Recruitment Strategies Stimulating Middle-Aged and Older Adults to Join Physical Activity Interventions
Authors: Boekhout Janet M. , Hut Rieteke , Lechner Lilian , Peels Denise A.
Published: June 2024

Many middle-aged and older adults (MAOAs) do not engage in sufficient physical activity (PA), despite its well-documented benefits for healthy aging. Existing PA interventions often fail to reach or engage the target population effectively. This study investigates MAOAs’ preferences for recruitment strategies to optimize the reach and uptake of PA interventions, thereby enhancing their impact on healthy aging and public health. Qualitative interviews were conducted with 39 MAOA participants (69% female, mean age = 69.46, SD = 7.07), guided by McGuire’s Theory on Persuasive Communication. Factors related to the source, message content, channel, receiver characteristics and target behavior of recruitment strategies were analyzed. Our findings suggest a preference for trustworthy sources (e.g., healthcare professionals over commercial entities) and positive, non-ageist messaging. MAOAs vary in their channel preferences but emphasize the importance of personalization. Despite heterogeneity, MAOAs commonly perceive themselves as sufficiently active, indicating a need for improved knowledge on what constitutes sufficient PA, as well as easy enrollment or trying out interventions. Tailoring recruitment strategies to diverse MAOA segments based on age seems crucial for effective engagement. Future research could explore quantitative research into how communication factors relate to various target population characteristics.
Patients’ Satisfaction Regarding Oral Healthcare Services in the North-East Region of Romania: A Preliminary Questionnaire Survey
Authors: Tibeica Silviu Catalin , Virvescu Dragoș Ioan , Lupu Iulian Costin , Budala Dana Gabriela , Luchian Ionut , Tibeica Andreea , Surlari Zinovia , Carausu Elena Mihaela
Published: June 2024

This research addresses a gap in the literature by conducting a comprehensive analysis of patients’ level of satisfaction with dental care. Methods: By combining quantitative and qualitative survey methods with a PSQ, this study aims to augment ongoing initiatives to enhance dental patients’ experiences by painting a more comprehensive depiction of patients’ level of satisfaction. Results: When asked about their overall level of satisfaction 77.1% of the patients said that they received excellent services from office personnel and 72.2% said they trust their doctors. Conclusions: Assessing patient satisfaction in the realm of dental service quality is crucial for enhancing service quality and accuracy, which would benefit both patients and dentists and, ultimately, improve public health.
Impact of Nutrition Education on Various Health-Related Components of Hemodialysis Patients: A Systematic Review
Authors: Ouirdani Marouane , Boutib Amal , Azizi Asmaa , Chergaoui Samia , Saad El Madani , Hilali Abderraouf , Marfak Abdelghafour , Youlyouz-Marfak Ibtissam
Published: June 2024

This study aimed to identify the impact of nutrition education on various health-related components of hemodialysis patients. A systematic review was conducted according to the PRISMA guidelines. Relevant literature published between 2013 and 2023 was identified across two databases (PubMed and Science Direct). The protocol was registered in PROSPERO (CRD42023460681). Two independent reviewers retrieved the data, and 41 studies were selected. Nine components related to the impact of nutrition education in hemodialysis patients were identified. Each component was clarified by mentioning each study and its results. This study enabled us to characterize the various components of the impact of nutritional education in hemodialysis patients, namely biological markers, quality of life, cost of care, adherence to dietary recommendations, knowledge, malnutrition inflammation, dietary intake, weight change, and behavior change. This systematic review enables healthcare providers to assess the impact of nutritional education on hemodialysis patients. Also, it gives professionals an exact idea of the impact of nutrition education on hemodialysis patients, with knowledge of new methods using behavior change theories and innovative technological tools.
Strategic Alliances in Global Health: Innovative Perspectives in the Era of Sustainable Development
Authors: Suarez-Herrera José Carlos , Abeldaño Zúñiga Roberto Ariel , Díaz-Castro Lina
Published: June 2024

This article discusses current challenges in the field of global health and the World Health Organization’s (WHO) strategies to address them. It highlights the importance of measuring the health impacts of global recession and globalization and the need for human-centered approaches to sustainable development. Emphasis is placed on commitment to health equity and the use of strategic partnerships for health at global, national, and local levels. Improving the health and well-being of populations, as well as public health equity, are core principles of the 2030 Agenda for the Sustainable Development Goals (SDGs). These principles are expressed in SDG 3, which promotes universal access to health services and systems and recognizes global health as a basic human right. It highlights the importance of strategic partnerships to combat emerging health crises, improve public health indices, and address the burden of chronic disease. These partnerships are contemplated in SDG 17 and are manifested in different modalities, such as network governance, cross-sector collaboration, public–private partnership, and social participation. This diversity of alliances has played an important role in scaling up and strengthening universal health systems around the world, including in Latin America and the Caribbean. The text concludes by presenting the essential characteristics of these inter-organizational and inter-institutional alliances in the field of global health.
Changes in Carotenoids and Polyphenols during the Growth Stages of Orange-Fleshed Sweet Potato (Ipomoea batatas (L.) Lam.)
Authors: Setoguchi Yuno , Narasako Yosuke , Hirano Tomonari , Otani Motoyasu , Kunitake Hisato
Published: June 2024

The storage roots of orange-fleshed sweet potato contain high levels of polyphenols and carotenoids. Little information is available on changes in the content and composition of these secondary metabolites during the growth stages of the root system. We investigated changes in carotenoids, polyphenols, and anthocyanins in the root system of the orange-fleshed sweet potato ‘Tamaakane’ from the post-planting stage. Carotenoids and polyphenols accumulated immediately after root-system formation at 15 days after transplanting (DAT). The levels of carotenoids increased by 45 DAT and decreased once the storage root enlargement began, but increased rapidly thereafter. After 90 DAT, β-carotene accounted for >90% of the total carotenoids, and the frequency remained stable until maturity. Total polyphenol content increased significantly towards 45 DAT and then gradually decreased as the storage roots began to thicken. No anthocyanin was detected in the root systems of ‘Tamaakane’ at any stage. The content and composition of these secondary metabolites are discussed in terms of agronomic aspects, as they might contribute to a strategy to protect storage roots while engaging with each other against biotic or abiotic stresses at the growth stage of the root system.
Iris pseudacorus and Lythrum anceps as Plants Supporting the Process of Removing Microplastics from Aquatic Environments—Preliminary Research
Authors: Kim Yoosun , Park Kiyoung , Bak Jonghyeok , Choi Sueran
Published: June 2024

(1) Background: Since microplastics in aquatic environments are difficult to prevent and can cause adverse physiological and biochemical reactions to various organisms, we aimed to analyze the effectiveness of using aquatic plants with well-developed roots and excellent water purification capabilities to remove microplastics in an eco-friendly manner. Additionally, we examined the differences in removal efficiency based on the sizes of the microplastic particles and the types of aquatic plants used. (2) Methods: Two types of polyethylene (PE) microplastic particles (46 µm and 140 µm) and two types of aquatic plants (Iris pseudacorus and Lythrum anceps) were used in this study. These plants were cultivated in tap water containing microplastics for a duration of four months in an aquatic setup without soil. Water samples from the cultivation area were analyzed using Fourier-transform infrared spectroscopy (FT-IR) to determine the reduction in microplastics. Scanning electron microscopy (SEM) was employed to examine the adsorption of microplastics on the plants’ roots. Plant growth was assessed by measuring plant height, plant width, and the number of branches (number of leaves). (3) Results: The results revealed significant reductions in the numbers of microplastics in the water of the cultivation boxes containing Iris pseudacorus and Lythrum anceps, irrespective of the microplastic particle size or plant type. These reductions were further confirmed by the adsorption of microplastics on the roots of both plant species. Moreover, the presence of microplastics had no significant negative effects on the plants’ growth. These findings suggest that Iris pseudacorus and Lythrum anceps are suitable plants for removing microplastics in aquatic environments. (4) Conclusions: To effectively reduce aquatic microplastics using plants, it is essential to establish a sustainable vegetation cover using perennial plants with well-developed roots and rapid reproductive capabilities. Follow-up research should consider not only the type of plant but also various aspects related to their tolerance to different environmental conditions.
Preliminary Study on Programmed Cell Death during Calyx Abscission of Korla Fragrant Pear
Authors: Wen Yue , Shao Baijunjie , Hao Zhichao , Wang Chunfeng , Sun Tianyu , Han Yutao , Tian Jia , Zhang Feng
Published: June 2024

Programmed cell death (PCD) is common in plant growth and development, such as xylem development, organ senescence, and abscission. Calyx abscission in Korla fragrant pear contributes to fruit quality, while it was not clear whether PCD occurred during calyx abscission and which signals regulated the process. Therefore, it is imperative to clarify the process of PCD in the process of calyx abscission in Korla fragrant pear under natural conditions to enrich the mechanism of calyx abscission. The results showed that the total time of calyx abscission of Korla fragrant pear began from 6 days after pollination (DAP) to 13 DAP, and the peak of calyx abscission occurred 10 DAP. During the whole process of calyx abscission, PCD started 6 DAP. At 9 DAP, the degree of PCD deepened. At 12 DAP, the cells in the abscission zone showed asymmetry on both sides, the organelles in the distal cells of the abscission zone degraded into apoptotic fragments, and the protective layer of the normal development of cells located at the proximal end of the abscission zone region ended the PCD process. ETH concentrations in the abscission zone of the decalyx fruit were significantly higher than those of the persistent calyx fruits in each period during calyx abscission, and high levels of ethylene and hydrogen peroxide and low contents of the GA3, ZT, and hydroxyl radicals promoted calyx abscission before the formation of the abscission zone. At 3 DAP, the ethylene concentration (43.97 ppm) and H2O2 content (8.49 μmol/g) of decalyx fruit in the abscission zone were significantly higher than those of persistent calyx fruit by 67.69% and 27.86%, respectively; however, the GA3, ZT, and hydroxyl radicals showed the opposite. Overall, PCD in the abscission zone of decalyx fruits did occur during the calyx abscission of Korla fragrant pear, and ethylene and H2O2 might play major roles in initiating the PCD process during Korla fragrant pear calyx abscission.
How Sage and Rosemary Essential Oils Regulate Postharvest Senescence and Extend the Vase Life of Cut Gladiolus Spikes
Authors: Moussa Mohamed M. , Mazrou Ragia M. , Hassan Fahmy A. S.
Published: June 2024

The production of cut flowers has substantial economic potential, and therefore, extending their lifespan has been the main focus of several floriculture researchers. Despite the increased marketable value of gladioli, their spikes rapidly lose their visual value and postharvest quality, accompanied by a short vase life. Unfortunately, most floral preservatives used to extend the flower lifespan have hazardous impacts; thus, providing eco-friendly alternatives has spurred immense interest among scientists. Sage and rosemary essential oils (EOs) seem to be effective eco-friendly flower preservatives due to their content of antimicrobial and antioxidant compounds. This study was therefore conducted to investigate whether using sage or rosemary EOs as novel preservative solutions can enhance the quality and prolong the vase life of cut gladiolus spikes. Gladiolus spikes were subjected to several concentrations (0, 50, 100, 150 and 200 mg L−1) of sage or rosemary EOs in a vase solution. All levels of both EOs significantly prolonged the vase life of gladiolus spikes, increased their water uptake and enhanced floret opening compared to the control. The vase life was increased by 88.16 and 84.76% by applying 150 or 100 mg L−1 of sage or rosemary EOs, respectively, compared to the untreated spikes. Sage and rosemary EO treatments markedly decreased bacterial populations, preserved the chlorophyll content, decreased H2O2 production and retarded the accumulation of malondialdehyde (MDA), and therefore preserved the membrane stability relative to the control. Furthermore, the total phenols and the antioxidant enzyme activities of catalase, glutathione reductase and ascorbate peroxidase were significantly increased due to sage or rosemary EO applications. In conclusion, sage or rosemary EOs may be applied as innovative, eco-friendly alternative preservatives to the communal chemicals used as preservatives in the cut flower industry.
Characterization of the Cassava (Manihot esculenta) Germplasm in Sierra Leone Based on Agro-Morphological and Culinary Traits
Authors: Amara Vandi , Samura Alusaine Edward , Norman Prince Emmanuel , Kanu Sheku Alfred , Karim Kumba Yannah , Tiendrebeogo Fidèle , Eni Angela Obiageli , Pita Justin S.
Published: June 2024

The dearth of information on the extent of genetic variability in cassava limits the genetic improvement of cassava in Sierra Leone. This study aimed at assessing the genetic variability and relationships within 103 cassava genotypes using agro-morphological and culinary markers. A field trial was conducted in the School of Agriculture and Food Sciences research site. The experiment was laid out in an augmented randomized block design. Morphological classification based on qualitative and quantitative traits categorized the germplasm into three different groups. A total of ten principal components (PCs) in the qualitative and six PCs in the quantitative trait sets accounted for 66.74% and 67.27% of the total genetic variation, respectively. Traits with significantly strong and positive correlations included presence of fruit (PFRT) and presence of seeds (PSE) (r = 0.86 ***), root yield per plant (RYPP) and number of storage roots (NSR) (r = 0.76 ***), RYPP and number of commercial roots (NCR) (r = 0.68 ***), length of leaf lobes (LLL) and width of leaf lobes (WLL) (r = 0.66 ***), and between NSR and NCR (r = 0.84 ***). The cooking time, cooking percentage, texture, mealiness, taste, and aroma varied widely among the accessions. The findings established that the cassava germplasm possesses useful genetic variability that could be exploited through selection for short-term release programs, genetic conservation of the valuable germplasm, and genetic improvement of the crop.
Use of a Biostimulant Based on Seaweed Extract as a Sustainable Input to Enhance the Quality of Solanaceous Seedlings
Authors: Villa e Vila Vinícius , Piedade Sônia Maria De Stefano , Bouix Christian Pascal , Rezende Roberto , Wenneck Gustavo Soares , Terassi Daniele de Souza , Matumoto-Pintro Paula Toshimi , Marques Patricia Angélica Alves
Published: June 2024

Seaweed extracts have several functions in agriculture due to their composition that is rich in nutrients, plant hormones, and bioactive substances. It is a natural product used as a biostimulant especially to promote the growth and development of plants and their tolerance to environmental stresses. The objective of this study was to analyze the response to a biostimulant containing seaweed extract derived from Ascophyllum nodosum in the cultivation of tomato and eggplant seedlings, analyzing the growth and physiological parameters in two different regions of Brazil. Cherry tomato and eggplant were cultivated in polyethylene trays for 30 days. In each crop, five treatments were tested, comparing the use of a commercial seaweed extract in application doses and forms, which were the control (without seaweed application); 0.1%, 0.2%, and 0.3% of the seaweed extract applied by irrigation water; and treatment with 0.2% of the seaweed extract by foliar application. This study confirms the efficacy of incorporating seaweed extract from Ascophyllum nodosum as a bio-input into the production phase of Solanaceae seedlings. The seedlings which received the seaweed extract significantly increased some morphological parameters, mainly regarding the biomass and length of leaves, stems, and roots. In general, applying both methods through irrigation water and foliar application were effective in providing benefits compared to the control treatment. The intermediate dose (0.2%) was the most effective in promoting improvement in the analyzed parameters. This underscores the importance of obtaining quality seedlings for subsequent planting in the field, potentially leading to better acclimatization and initial adaptation.
Automatic Extraction and Cluster Analysis of Natural Disaster Metadata Based on the Unified Metadata Framework
Authors: Wang Zongmin , Shi Xujie , Yang Haibo , Yu Bo , Cai Yingchun
Published: June 2024

The development of information technology has led to massive, multidimensional, and heterogeneously sourced disaster data. However, there’s currently no universal metadata standard for managing natural disasters. Common pre-training models for information extraction requiring extensive training data show somewhat limited effectiveness, with limited annotated resources. This study establishes a unified natural disaster metadata standard, utilizes self-trained universal information extraction (UIE) models and Python libraries to extract metadata stored in both structured and unstructured forms, and analyzes the results using the Word2vec-Kmeans cluster algorithm. The results show that (1) the self-trained UIE model, with a learning rate of 3 × 10−4 and a batch_size of 32, significantly improves extraction results for various natural disasters by over 50%. Our optimized UIE model outperforms many other extraction methods in terms of precision, recall, and F1 scores. (2) The quality assessments of consistency, completeness, and accuracy for ten tables all exceed 0.80, with variances between the three dimensions being 0.04, 0.03, and 0.05. The overall evaluation of data items of tables also exceeds 0.80, consistent with the results at the table level. The metadata model framework constructed in this study demonstrates high-quality stability. (3) Taking the flood dataset as an example, clustering reveals five main themes with high similarity within clusters, and the differences between clusters are deemed significant relative to the differences within clusters at a significance level of 0.01. Overall, this experiment supports effective sharing of disaster data resources and enhances natural disaster emergency response efficiency.
Naphthoquinone-Quinolone Hybrids with Antitumor Effects on Breast Cancer Cell Lines—From the Synthesis to 3D-Cell Culture Effects
Authors: da Gama Oliveira Vanessa , Muxfeldt Marcelly , Muniz da Paz Mariana , Silva Coutinho Mayra , Eduardo dos Santos Raissa , Diniz da Silva Ferretti Giulia , Ferraz da Costa Danielly C. , Fonseca Regufe Pedro , Lelis Gama Ivson , da Costa Santos Boechat Fernanda , Silva Lima Emersom , Ferreira Vitor Francisco , de Moraes Marcela Cristina , Bastos Vieira de Souza Maria Cecília , Netto Batalha Pedro , Pereira Rangel Luciana
Published: June 2024

Breast cancer stands as one of the foremost cause of cancer-related deaths globally, characterized by its varied molecular subtypes. Each subtype requires a distinct therapeutic strategy. Although advancements in treatment have enhanced patient outcomes, significant hurdles remain, including treatment toxicity and restricted effectiveness. Here, we explore the anticancer potential of novel 1,4-naphthoquinone/4-quinolone hybrids on breast cancer cell lines. The synthesized compounds demonstrated selective cytotoxicity against Luminal and triple-negative breast cancer (TNBC) cells, which represent the two main molecular types of breast cancer that depend most on cytotoxic chemotherapy, with potency comparable to doxorubicin, a standard chemotherapeutic widely used in breast cancer treatment. Notably, these derivatives exhibited superior selectivity indices (SI) when compared to doxorubicin, indicating lower toxicity towards non-tumor MCF10A cells. Compounds 11a and 11b displayed an improvement in IC50 values when compared to their precursor, 1,4-naphthoquinone, for both MCF-7 and MDA-MB-231 and a comparable value to doxorubicin for MCF-7 cells. Also, their SI values were superior to those seen for the two reference compounds for both cell lines tested. Mechanistic studies revealed the ability of the compounds to induce apoptosis and inhibit clonogenic potential. Additionally, the irreversibility of their effects on cell viability underscores their promising therapeutic utility. In 3D-cell culture models, the compounds induced morphological changes indicative of reduced viability, supporting their efficacy in a more physiologically relevant model of study. The pharmacokinetics of the synthesized compounds were predicted using the SwissADME webserver, indicating that these compounds exhibit favorable drug-likeness properties and potential as antitumor agents. Overall, our findings underscore the promise of these hybrid compounds as potential candidates for breast cancer chemotherapy, emphasizing their selectivity and efficacy.
Modeling of Blood–Brain Barrier (BBB) Dysfunction and Immune Cell Migration Using Human BBB-on-a-Chip for Drug Discovery Research
Authors: Ohbuchi Masato , Shibuta Mayu , Tetsuka Kazuhiro , Sasaki-Iwaoka Haruna , Oishi Masayo , Shimizu Fumitaka , Nagasaka Yasuhisa
Published: June 2024

Blood–brain barrier (BBB) dysfunction is a key feature in neuroimmunological and neurodegenerative diseases. In this study, we developed a microfluidic human BBB-on-a-chip to model barrier dysfunction and immune cell migration using immortalized TY10 brain endothelial cells, pericytes, and astrocytes. It was found that immortalized TY10 brain endothelial cells developed a microvascular structure under flow. Pericytes were localized on the basal side surrounding the TY10 microvascular structure, showing an in vivo-like structure. Barrier integrity increased under co-culture with pericytes. In addition, both ethylenediaminetetraacetic acid (EDTA) and anti-Claudin-5 (CLDN5) neutralizing antibody caused a decrease in the transendothelial electrical resistance (TEER). EDTA caused the leakage of 20 kDa dextran, suggesting different effects on the BBB based on the mechanism of action, whereas anti-CLDN5 antibody did not cause leakage. In the tri-culture model, human T cells migrated through endothelial vessels towards basal C-X-C motif chemokine ligand 12 (CXCL12). The live-imaging analysis confirmed the extravasation of fluorescence-labelled T cells in a CXCL12-concentration- and time-dependent manner. Our BBB model had an in vivo-like structure and successfully represented barrier dysfunction and transendothelial T cell migration. In addition, our study suggests that the inhibition of CLDN5 attenuates the BBB in humans. This platform has various potential uses in relation to the BBB in both drug discovery research and in elucidating the mechanisms of central nervous system diseases.
The Application of Machine Learning in Diagnosing the Financial Health and Performance of Companies in the Construction Industry
Authors: Horváthová Jarmila , Mokrišová Martina , Schneider Alexander
Published: June 2024

Diagnosing the financial health of companies and their performance is currently one of the basic questions that attracts the attention of researchers and experts in the field of finance and management. In this study, we focused on the proposal of models for measuring the financial health and performance of businesses. These models were built for companies doing business within the Slovak construction industry. Construction companies are identified by their higher liquidity and different capital structure compared to other industries. Therefore, simple classifiers are not able to effectively predict their financial health. In this paper, we investigated whether boosting ensembles are a suitable alternative for performance analysis. The result of the research is the finding that deep learning is a suitable approach aimed at measuring the financial health and performance of the analyzed sample of companies. The developed models achieved perfect classification accuracy when using the AdaBoost and Gradient-boosting algorithms. The application of a decision tree as a base learner also proved to be very appropriate. The result is a decision tree with adequate depth and very good interpretability.
Mass and Stiffness Correlation Using a Transformation Matrix
Authors: García Fernández Natalia , Fernández Fernandez Pelayo , Brincker Rune , Aenlle López Manuel
Published: June 2024

Model correlation techniques are methods used to compare two different models, usually a numerical model and an experimental model. According to the structural dynamic modification theory, the experimental mode shapes estimated by modal analysis can be expressed as a linear combination of the numerical mode shapes through a transformation matrix T. In this paper, matrix T is proposed as a novel model correlation technique to detect discrepancies between the numerical and the experimental models in terms of mass. The discrepancies in stiffness can be identified by combining the numerical natural frequencies and the matrix T. This methodology can be applied to correlate the numerical and experimental results of civil (bridges, dams, towers, buildings, etc.), aerospace and mechanical structures and to detect damage when using structural health monitoring techniques. The technique was validated by numerical simulations on a lab-scaled two-span bridge considering different degradation scenarios and experimentally on a lab-scaled structure, which was correlated with two numerical models.
Impact of Ae-GRD on Ivermectin Resistance and Its Regulation by miR-71-5p in Aedes aegypti
Authors: Yu Lingling , Yin Yanan , Wang Qiuhui , Zhao Peizhen , Han Qian , Liao Chenghong
Published: June 2024

iGABAR, a member of the Cys-loop ligand-gated ion channel superfamily, is a significant target of the insecticide ivermectin (IVM). GRD is the potential subunit of the insect iGABAR. However, little information about GRD in Ae. aegypti has been reported. In this study, we involved cloning and characterizing the iGABAR subunit GRD of Ae. aegypti (Ae-GRD). Sequence analysis indicated that Ae-GRD, as part of the cysteine-loop ligand-gated ion channel family, is similar to other insect GRD. RNA interference (RNAi) was employed to explore IVM resistance in Ae. aegypti, resulting in a significant reduction in Ae-GRD expression (p < 0.05), and the mortality of Ae. aegypti adults with Ae-GRD knockdown was significantly decreased after exposure to ivermectin. Bioinformatics prediction identified miR-71-5p as a potential regulator of Ae-GRD. In vitro, dual-luciferase reporter assays confirmed that Ae-GRD expression was regulated by miR-71-5p. Microinjection of miR-71-5p mimics upregulated miR-71-5p expression and downregulated Ae-GRD gene expression, reducing mortality by 34.52% following IVM treatment. Conversely, microinjection of a miR-71-5p inhibitor decreased miR-71-5p expression but did not affect the susceptibility to IVM despite increased Ae-GRD expression (p < 0.05). In conclusion, Ae-GRD, as one of the iGABA receptor subunits, is a potential target of ivermectin. It may influence ivermectin resistance by modulating the GABA signaling pathway. The inhibition of Ae-GRD expression by miR-71-5p decreased ivermectin resistance and consequently lowered the mortality rate of Ae. aegypti mosquitoes. This finding provides empirical evidence of the relationship between Ae-GRD and its miRNA in modulating insecticide resistance, offering novel perspectives for mosquito control strategies.
Contrast-Enhanced Magnetic Resonance Imaging Based T1 Mapping and Extracellular Volume Fractions Are Associated with Peripheral Artery Disease
Authors: Fitian Asem I. , Shieh Michael C. , Gimnich Olga A. , Belousova Tatiana , Taylor Addison A. , Ballantyne Christie M. , Bismuth Jean , Shah Dipan J. , Brunner Gerd
Published: June 2024

Background: Extracellular volume fraction (ECV), measured with contrast-enhanced magnetic resonance imaging (CE-MRI), has been utilized to study myocardial fibrosis, but its role in peripheral artery disease (PAD) remains unknown. We hypothesized that T1 mapping and ECV differ between PAD patients and matched controls. Methods and Results: A total of 37 individuals (18 PAD patients and 19 matched controls) underwent 3.0T CE-MRI. Skeletal calf muscle T1 mapping was performed before and after gadolinium contrast with a motion-corrected modified look–locker inversion recovery (MOLLI) pulse sequence. T1 values were calculated with a three-parameter Levenberg–Marquardt curve fitting algorithm. ECV and T1 maps were quantified in five calf muscle compartments (anterior [AM], lateral [LM], and deep posterior [DM] muscle groups; soleus [SM] and gastrocnemius [GM] muscles). Averaged peak blood pool T1 values were obtained from the posterior and anterior tibialis and peroneal arteries. T1 values and ECV are heterogeneous across calf muscle compartments. Native peak T1 values of the AM, LM, and DM were significantly higher in PAD patients compared to controls (all p < 0.028). ECVs of the AM and SM were significantly higher in PAD patients compared to controls (AM: 26.4% (21.2, 31.6) vs. 17.3% (10.2, 25.1), p = 0.046; SM: 22.7% (19.5, 27.8) vs. 13.8% (10.2, 19.1), p = 0.020). Conclusions: Native peak T1 values across all five calf muscle compartments, and ECV fractions of the anterior muscle group and the soleus muscle were significantly elevated in PAD patients compared with matched controls. Non-invasive T1 mapping and ECV quantification may be of interest for the study of PAD.
Chronic Kidney Disease Increases Mortality and Reduces the Chance of a Favorable Outcome in Stroke Patients Treated with Mechanical Thrombectomy—Single-Center Study
Authors: Borończyk Michał , Kuźniak Mikołaj , Borończyk Agnieszka , Barański Kamil , Hawrot-Kawecka Anna , Lasek-Bal Anetta
Published: June 2024

Background/Objectives: Chronic kidney disease (CKD) is identified as a risk factor for the occurrence of ischemic stroke. There is substantial evidence that CKD is linked to a worse prognosis and higher mortality rates in stroke patients. This study aimed to evaluate the characteristics and factors affecting favorable outcomes and mortality in patients treated using mechanical thrombectomy (MT) for ischemic stroke, with particular emphasis on patients suffering from CKD. Methods: The retrospective study included an analysis of data from 723 patients (139; 19.4% had CKD) with ischemic stroke treated with MT between March 2019 and July 2022. Results: Patients with CKD were significantly older (median age 76.5 vs. 65.65, p < 0.001) and more often female (59.7% vs. 42.6%, p < 0.001). CKD decreased the likelihood of achieving a favorable outcome (0–2 points in modified Rankin scale; OR: 0.56, CI95%: 0.38–0.81) and increased mortality (OR: 2.59, CI95%: 1.74–3.84) on the 90th day after stroke. In addition, CKD was associated with intracranial hemorrhage (ICH) in patients who underwent posterior circulation MT (13.85% vs. 50%, p = 0.022). In patients with CKD, inter alia, higher levels of C-reactive protein (OR: 0.94, CI95%: 0.92–0.99) reduced the chance of a favorable outcome. In addition, the occurrence of ICH in patients with CKD increased mortality on the 90th day after stroke (OR: 4.18, CI95%: 1.56–11.21), which was almost twice as high as in patients without CKD (OR: 2.29, CI95%: 1.54–3.40). Conclusions: Patients suffering from CKD had a lower probability of achieving a favorable outcome and had increased mortality following MT for ischemic stroke. It is crucial to understand the variations between patients with unimpaired and impaired renal function, as this could aid in predicting the outcomes of this method.
Sodium-Glucose Cotransporter Type 2 Inhibitors Use in Elderly Polypathological Patients with Acute Heart Failure: PROFUND-IC Registry
Authors: Guzmán-Carreras Alicia , Vellisca-González Andrea María , Molina-Puente Juan Igor , García-Alonso Rocío , Paz-Cabezas Mateo , Sánchez-Sauce Beatriz , Aguilar-Rodríguez Fernando , Iguarán-Bermúdez María Del Rosario , Andrès Emmanuel , Lorenzo-Villalba Noel , Méndez-Bailón Manuel
Published: June 2024

Background/Objectives: Heart failure (HF) is a highly prevalent clinical syndrome with serious morbidity and mortality. Furthermore, acute heart failure (AHF) is the main cause of hospital admission in people aged 65 years or more. Sodium-glucose cotransporter type 2 inhibitors (SGLT2is) have been shown to improve the survival and quality of life in patients with HF regardless of left ventricular ejection fraction (LVEF). Our aims were to describe the characteristics of adults with multiple pathologies admitted with acute heart failure as the main diagnosis and of the population treated with SGLT2is, as well as to evaluate if their use was associated with lower readmission and mortality rates. Methods: A prospective study of patients from the PROFUND-IC registry who were admitted with AHF as the main diagnosis was conducted. Clinical and analytical characteristics were analyzed, as well as readmissions and mortality. Descriptive and bivariate analyses of the sample between those taking SGLT2is and those who were not were performed, using the chi-square test for qualitative variables and Welch’s test for quantitative measures, as well as the Fisher and Wilcoxon tests as indicated for nonparametric tests. Kaplan–Meier curves were constructed to analyze the readmission and mortality of patients at 12 months based on SGLT2i treatment. Finally, a propensity score matching was performed, guaranteeing that the observed effect of the drug was not influenced by the differences in the characteristics between the groups. Results: There were 750 patients included: 58% were women, and the mean age was 84 years. Functional class II according to the NYHA scale predominated (54%), and the mean LVEF was 51%. SGLT2 inhibitors were prescribed to only 28% of patients. Most of the patients were men (48.6% vs. 39.8%, p = 0.029), they were younger (82 vs. 84 years, p = 0.002), and their LVEF was lower (48% vs. 52%, p < 0.001). Lower mortality was observed in the group treated with SGLT2is, both during baseline admission (2.4% vs. 6.9%, p = 0.017) and at the 12-month follow-up (6.2% vs. 13%, p = 0.023); as well as a lower readmission rate (23.8% vs. 38.9%, p < 0.001). After the propensity score matching, a decrease in the 12-month readmission rate continued to be observed in the group treated with SGLT2is (p = 0.03). Conclusions: SGLT2is use was associated with lower readmission rates at the 12-month follow-up in older adults with multiple pathologies admitted with acute heart failure.