bluuebunny/crossref_metadata_2025 huggingface dataset:
Reconciling Simulations and Experiments With BICePs: A Review
Authors: Voelz Vincent A. , Ge Yunhui , Raddi Robert M.
Published: May 2021

Bayesian Inference of Conformational Populations (BICePs) is an algorithm developed to reconcile simulated ensembles with sparse experimental measurements. The Bayesian framework of BICePs enables population reweighting as a post-simulation processing step, with several advantages over existing methods, including the proper use of reference potentials, and the estimation of a Bayes factor-like quantity called the BICePs score for model selection. Here, we summarize the theory underlying this method in context with related algorithms, review the history of BICePs applications to date, and discuss current shortcomings along with future plans for improvement.
Stress-Responsive Periplasmic Chaperones in Bacteria
Authors: Kim Hyunhee , Wu Kevin , Lee Changhan
Published: May 2021

Periplasmic proteins are involved in a wide range of bacterial functions, including motility, biofilm formation, sensing environmental cues, and small-molecule transport. In addition, a wide range of outer membrane proteins and proteins that are secreted into the media must travel through the periplasm to reach their final destinations. Since the porous outer membrane allows for the free diffusion of small molecules, periplasmic proteins and those that travel through this compartment are more vulnerable to external environmental changes, including those that result in protein unfolding, than cytoplasmic proteins are. To enable bacterial survival under various stress conditions, a robust protein quality control system is required in the periplasm. In this review, we focus on several periplasmic chaperones that are stress responsive, including Spy, which responds to envelope-stress, DegP, which responds to temperature to modulate chaperone/protease activity, HdeA and HdeB, which respond to acid stress, and UgpB, which functions as a bile-responsive chaperone.
Social Experience Regulates Endocannabinoids Modulation of Zebrafish Motor Behaviors
Authors: Orr Stephen A. , Ahn Sungwoo , Park Choongseok , Miller Thomas H. , Kassai Miki , Issa Fadi A.
Published: May 2021

Social status-dependent modulation of neural circuits has been investigated extensively in vertebrate and invertebrate systems. However, the effects of social status on neuromodulatory systems that drive motor activity are poorly understood. Zebrafish form a stable social relationship that consists of socially dominant and subordinate animals. The locomotor behavior patterns differ according to their social ranks. The sensitivity of the Mauthner startle escape response in subordinates increases compared to dominants while dominants increase their swimming frequency compared to subordinates. Here, we investigated the role of the endocannabinoid system (ECS) in mediating these differences in motor activities. We show that brain gene expression of key ECS protein pathways are socially regulated. Diacylglycerol lipase (DAGL) expression significantly increased in dominants and significantly decreased in subordinates relative to controls. Moreover, brain gene expression of the cannabinoid 1 receptor (CB1R) was significantly increased in subordinates relative to controls. Secondly, increasing ECS activity with JZL184 reversed swimming activity patterns in dominant and subordinate animals. JZL184 did not affect the sensitivity of the startle escape response in dominants while it was significantly reduced in subordinates. Thirdly, blockage of CB1R function with AM-251 had no effect on dominants startle escape response sensitivity, but startle sensitivity was significantly reduced in subordinates. Additionally, AM-251 did not affect swimming activities in either social phenotypes. Fourthly, we demonstrate that the effects of ECS modulation of the startle escape circuit is mediated via the dopaminergic system specifically via the dopamine D1 receptor. Finally, our empirical results complemented with neurocomputational modeling suggest that social status influences the ECS to regulate the balance in synaptic strength between excitatory and inhibitory inputs to control the excitability of motor behaviors. Collectively, this study provides new insights of how social factors impact nervous system function to reconfigure the synergistic interactions of neuromodulatory pathways to optimize motor output.
Glioma Biopsy Based on Hybrid Dual Time-Point FET-PET/MRI—A Proof of Concept Study
Authors: Furtak Jacek , Rakowska Józefina , Szylberg Tadeusz , Harat Marek , Małkowski Bogdan , Harat Maciej
Published: May 2021

Neuroimaging based on O-[2-(18F)fluoroethyl]-l-tyrosine (FET)-PET provides additional information on tumor grade and extent compared with MRI. Dynamic PET for biopsy target selection further improves results but is often clinically impractical. Static FET-PET performed at two time-points may be a good compromise, but data on this approach are limited. The aim of this study was to compare the histology of lesions obtained from two challenging glioma patients with targets selected based on hybrid dual time-point FET-PET/MRI. Five neuronavigated tumor biopsies were performed in two difficult cases of suspected glioma. Lesions with (T1-CE) and without contrast enhancement (T1 and T2-FLAIR) on MRI were selected. Dual time-point FET-PET imaging was performed 5–15 min (PET10) and 45–60 min (PET60) after radionuclide injection. The most informative FET-PET/MRI images were coregistered with MRI in time of biopsy planning. Five biopsy targets (three from high uptake and two from moderate uptake FET areas) thought to represent the most malignant sites and tumor extent were selected. Histopathological findings were compared with FET-PET and MRI images. Increased FET uptake in the area of non-CE locations on MRI correlated well with high-grade gliomas localized as far as 3 cm from T1-CE foci. Selecting a target in the motor cortex based on FET kinetics defined by dual time-point PET resulted in a grade IV diagnosis after previous negative biopsies based on MRI. An additional grade III diagnosis was obtained from an area of glioma infiltration with moderate FET uptake (between 1 and 1.25 SUV). These findings seem to show that dual time-point FET-PET-based biopsies can provide additional and clinically useful information for glioma diagnosis. Selection of targets based on dual time-point images may be useful for determining the most malignant tumor areas and may therefore be useful for resection and radiotherapy planning.
Colors and Handles: How Action Primes Perception
Authors: Costantini Marcello , Quarona Davide , Sinigaglia Corrado
Published: May 2021

How deeply does action influence perception? Does action performance affect the perception of object features directly related to action only? Or does it concern also object features such as colors, which are not held to directly afford action? The present study aimed at answering these questions. We asked participants to repeatedly grasp a handled mug hidden from their view before judging whether a visually presented mug was blue rather than cyan. The motor training impacted on their perceptual judgments, by speeding participants’ responses, when the handle of the presented mug was spatially aligned with the trained hand. The priming effect did not occur when participants were trained to merely touch the mug with their hand closed in a fist. This indicates that action performance may shape the perceptual judgment on object features, even when these features are colors and do not afford any action. How we act on surrounding objects is therefore not without consequence for how we experience them.
Tiered Neuroscience and Mental Health Professional Development in Liberia Improves Teacher Self-Efficacy, Self-Responsibility, and Motivation
Authors: Brick Kara , Cooper Janice L. , Mason Leona , Faeflen Sangay , Monmia Josiah , Dubinsky Janet M.
Published: May 2021

After acquiring knowledge of the neuroscience of learning, memory, stress and emotions, teachers incorporate more cognitive engagement and student-centered practices into their lessons. However, the role understanding neuroscience plays in teachers own affective and motivational competencies has not yet been investigated. The goal of this study was to investigate how learning neuroscience effected teachers’ self-efficacy, beliefs in their ability to teach effectively, self-responsibility and other components of teacher motivation. A pilot training-of-trainers program was designed and delivered in Liberia combining basic neuroscience with information on social, emotional, behavioral and mental health issues faced by students. Tier I of the professional development was a 2 weeks workshop led by a visiting neuroscientist. A subset of the 24 Tier I secondary science teachers formed a Leadership Team who adapted the content to the Liberian context and subsequently led additional workshops and follow-up sessions for the Tier II secondary science teachers. Science teachers in both tiers completed the affective-motivational scales from the internationally vetted, multiscale Innovative Teaching for Effective Learning Teacher Knowledge Survey from the OECD. Tier II teachers completed the survey in a pre-post-delayed post design. Tier I teachers completed the survey after the workshop with their attitudes at that time and separately with retrospective projections of their pre-workshop attitudes. Ten of the 92 Tier II teachers participated in structured interviews at follow-up. Statistical analysis of survey data demonstrated improved teacher self-efficacy, self-responsibility for student outcomes, and motivation to teach. Qualitatively, teachers expressed more confidence in their ability to motivate students, engage them through active learning, and manage the class through positive rather than negative reinforcement. Teachers’ own self-regulation improved as they made efforts to build supporting relationships with students. Together, these results demonstrated that (i) teacher affective-motivational attitudes can be altered with professional development, (ii) basic neuroscience, as knowledge of how students learn, can improve teacher competency, and (iii) a training-of-trainers model can be effective in a low and middle income country for disseminating neuroscience knowledge, increasing teachers’ knowledge of students’ social and emotional needs, and promoting educational improvement.
Sex-Dependent Effects of Chronic Social Defeat on Emotional and Social Behaviors, and Parameters of Oxytocin and Vasopressin Systems in Mandarin Voles (Microtus mandarinus)
Authors: Hou Wenjuan , Ma Huan , Xun Yufeng , Zhang Xin , Cai Wenqi , Huang Shuying , He Zhixiong , Tai Fadao , Jia Rui
Published: May 2021

In the regulation of emotional and social behaviors, both oxytocin (OT) and vasopressin (AVP) are sex specific. Although significant sex differences have been reported in the context of behavioral and hormonal responses to social stress, such differences in response to chronic social defeat stress (CSDS) and the underlying neural mechanisms remain largely unknown. By investigating monogamous mandarin voles (Microtus mandarinus), CSDS was found to decrease the percentages of time spent in the central area of the open field, in the open arms of the elevated plus maze, as well as in the light area of the light and dark boxes in both male and female voles. CSDS also increased the observed level of social withdrawal in both sex groups. However, CSDS exposure increased the percentages of immobile time in both the tail suspension test and the forced swim test and reduced the locomotor activity in the open field (in females only). Along with these behavioral changes, the oxytocin receptor (OTR) levels in the nucleus accumbens (NAc) were significantly lower in CSDS-exposed voles of both sexes; however, in males, the levels of OTR in the paraventricular nucleus (PVN) were reduced. CSDS-exposed males showed lower levels of V1aR in the NAc than CSDS-exposed females. Furthermore, induced by a single social defeat event, CSDS reduced c-Fos and OT double labeling in the PVN of females but increased c-Fos and AVP double-labeled neurons in the PVN of males exposed to a single social defeat event. Collectively, the present study indicates that OT and AVP systems may play important regulatory roles in the sex differences of behavioral performances in response to CSDS. These findings suggest mandarin voles as a useful animal model for studying sex-specific behavioral performance and the underlying neurobiological mechanisms of stress-related mental disorders in preclinical studies.
A Novel Statistical Model for Predicting the Efficacy of Vagal Nerve Stimulation in Patients With Epilepsy (Pre-X-Stim) Is Applicable to Different EEG Systems
Authors: Korit́áková Eva , Doležalová Irena , Chládek Jan , Jurková Tereza , Chrastina Jan , Plešinger Filip , Roman Robert , Pail Martin , Jurák Pavel , Shaw Daniel J. , Brázdil Milan
Published: May 2021

Background: Identifying patients with intractable epilepsy who would benefit from therapeutic chronic vagal nerve stimulation (VNS) preoperatively remains a major clinical challenge. We have developed a statistical model for predicting VNS efficacy using only routine preimplantation electroencephalogram (EEG) recorded with the TruScan EEG device (Brazdil et al., 2019). It remains to be seen, however, if this model can be applied in different clinical settings.

Objective: To validate our model using EEG data acquired with a different recording system.

Methods: We identified a validation cohort of eight patients implanted with VNS, whose preimplantation EEG was recorded on the BrainScope device and who underwent the EEG recording according to the protocol. The classifier developed in our earlier work, named Pre-X-Stim, was then employed to classify these patients as predicted responders or non-responders based on the dynamics in EEG power spectra. Predicted and real-world outcomes were compared to establish the applicability of this classifier. In total, two validation experiments were performed using two different validation approaches (single classifier or classifier voting).

Results: The classifier achieved 75% accuracy, 67% sensitivity, and 100% specificity. Only two patients, both real-life responders, were classified incorrectly in both validation experiments.

Conclusion: We have validated the Pre-X-Stim model on EEGs from a different recording system, which indicates its application under different technical conditions. Our approach, based on preoperative EEG, is easily applied and financially undemanding and presents great potential for real-world clinical use.
Deep Neural Network for Differentiation of Brain Tumor Tissue Displayed by Confocal Laser Endomicroscopy
Authors: Ziebart Andreas , Stadniczuk Denis , Roos Veronika , Ratliff Miriam , von Deimling Andreas , Hänggi Daniel , Enders Frederik
Published: May 2021

Background

Reliable on site classification of resected tumor specimens remains a challenge. Implementation of high-resolution confocal laser endoscopic techniques (CLEs) during fluorescence-guided brain tumor surgery is a new tool for intraoperative tumor tissue visualization. To overcome observer dependent errors, we aimed to predict tumor type by applying a deep learning model to image data obtained by CLE.

Methods

Human brain tumor specimens from 25 patients with brain metastasis, glioblastoma, and meningioma were evaluated within this study. In addition to routine histopathological analysis, tissue samples were stained with fluorescein ex vivo and analyzed with CLE. We trained two convolutional neural networks and built a predictive level for the outputs.

Results

Multiple CLE images were obtained from each specimen with a total number of 13,972 fluorescein based images. Test accuracy of 90.9% was achieved after applying a two-class prediction for glioblastomas and brain metastases with an area under the curve (AUC) value of 0.92. For three class predictions, our model achieved a ratio of correct predicted label of 85.8% in the test set, which was confirmed with five-fold cross validation, without definition of confidence. Applying a confidence rate of 0.999 increased the prediction accuracy to 98.6% when images with substantial artifacts were excluded before the analysis. 36.3% of total images met the output criteria.

Conclusions

We trained a residual network model that allows automated, on site analysis of resected tumor specimens based on CLE image datasets. Further in vivo studies are required to assess the clinical benefit CLE can have.
Case Report: Effects of Secondary Hyperparathyroidism Treatment on Improvement of Juvenile Nephronophthisis-Induced Pancytopenia and Myelofibrosis
Authors: Amano Keishiro , Toyoda Hidemi , Nishikawa Kouhei , Murata Tomohiro , Hirayama Masahiro
Published: May 2021

Secondary hyperparathyroidism (HPT) is a common complication of end-stage renal disease (ESRD) and may be an important precipitating factor for the development of myelofibrosis. However, there have been only a few reports on myelofibrosis caused by secondary HPT in children. We describe a case of a 15-year-old boy with myelofibrosis due to secondary HPT who was successfully treated with hemodialysis, erythropoietin, phosphate binders, and activated vitamin D agents. The patient had no past medical history and had been admitted to the hospital for abdominal pain. Routine blood examination revealed pancytopenia combined with renal impairment. Hyperphosphatemia, decreased 1,25-dehydroxyvitamin D, decreased serum calcium, and increased parathyroid hormone (PTH) levels were observed. Bone marrow biopsy confirmed myelofibrosis and renal biopsy revealed nephronophthisis (NPHP). The possibility of renal osteodystrophy and myelofibrosis due to secondary HPT was considered. Hemodialysis and erythropoietin were initiated and combined therapy with a phosphate binder and an active vitamin D agent achieved greater reduction of PTH levels, along with improvement of pancytopenia. As medical treatment for secondary HPT can lead to a reversal of myelofibrosis and avoid parathyroidectomy in children, prompt recognition of this condition has major implications for treatment. Therefore, despite its rarity, pediatricians should consider myelofibrosis due to secondary HPT as a cause of pancytopenia in patients with chronic kidney disease.
Adverse Events and Unsuccessful Intubation Attempts Are Frequent During Neonatal Nasotracheal Intubations
Authors: Tippmann Susanne , Haan Martin , Winter Julia , Mühler Ann-Kathrin , Schmitz Katharina , Schönfeld Mascha , Brado Luise , Mahmoudpour Seyed Hamidreza , Mildenberger Eva , Kidszun André
Published: May 2021

Background: Intubation of neonates is difficult and hazardous. Factors associated with procedure-related adverse events and unsuccessful intubation attempts are insufficiently evaluated, especially during neonatal nasotracheal intubations.

Objective: Aim of this study was to determine the frequency of tracheal intubation–associated events (TIAEs) during neonatal nasotracheal intubations and to identify factors associated with TIAEs and unsuccessful intubation attempts in our neonatal unit.

Methods: This was a prospective, single-site, observational study from May 2017 to November 2019, performed at a tertiary care neonatal intensive care unit in a German academic teaching hospital. All endotracheal intubation encounters performed by the neonatal team were recorded.

Results: Two hundred and fifty-eight consecutive intubation encounters in 197 patients were analyzed. One hundred and forty-eight (57.4%) intubation encounters were associated with at least one TIAE. Intubation inexperience (<10 intubation encounters) (OR = 2.15; 95% CI, 1.257–3.685) and equipment problems (OR = 3.43; 95% CI, 1.12–10.52) were predictive of TIAEs. Intubation at first attempt (OR = 0.10; 95% CI, 0.06–0.19) and videolaryngoscopy (OR = 0.47; 96% CI, 0.25–0.860) were predictive of intubation encounters without TIAEs. The first intubation attempt was commonly done by pediatric residents (67.8%). A median of two attempts were performed until successful intubation. Restricted laryngoscopic view (OR = 3.07; 95% CI, 2.08–4.53; Cormack-Lehane grade 2 vs. grade 1), intubation by pediatric residents when compared to neonatologists (OR = 1.74; 95% CI, 1.265–2.41) and support by less experienced neonatal nurses (OR = 1.60; 95% CI, 1.04–2.46) were associated with unsuccessful intubation attempts.

Conclusions: In our unit, TIAEs and unsuccessful intubation attempts occurred frequently during neonatal nasotracheal intubations. To improve success rates, quality improvement und further research should target interprofessional education and training, equipment problems and videolaryngoscopy.
Synergistic Effect of Cefazolin Plus Fosfomycin Against Staphylococcus aureus in vitro and in vivo in an Experimental Galleria mellonella Model
Authors: Kussmann Manuel , Obermueller Markus , Karer Matthias , Kriz Richard , Chen Rui-Yang , Hohl Lena , Schneider Lisa , Burgmann Heinz , Traby Ludwig , Vossen Matthias G.
Published: May 2021

Objectives: This study investigated the synergistic in vitro and in vivo activity of cefazolin plus fosfomycin against methicillin-susceptible and methicillin-resistant S. aureus (MSSA and MRSA) to provide the basis for a potential treatment alternative.

Methods: Antimicrobial susceptibility and in vitro synergy tests were performed with five MSSA and five MRSA isolates using the broth microdilution and chequerboard assays, respectively. The in vivo efficacy of cefazolin plus fosfomycin for the treatment of MRSA infections was assessed using the Galleria mellonella survival assay.

Results: Using fractional inhibitory concentration index (FICI), the evaluated combination of cefazolin plus fosfomycin showed synergistic in vitro activity against all MSSA and MRSA isolates tested. In addition, cefazolin susceptibility was recovered in all MRSA isolates except one fosfomycin-resistant strain when combined with fosfomycin at readily achievable concentrations. The G. mellonella survival assay demonstrated highly synergistic in vivo activity of cefazolin plus fosfomycin, resulting in a 44–52% reduction in mortality when compared to cefazolin-alone and fosfomycin-alone, respectively.

Conclusion: If susceptibility to fosfomycin is either confirmed or can be assumed based on local resistance patterns, combination therapy with cefazolin plus fosfomycin could be a valuable treatment option for empirical as well as targeted therapy of S. aureus and MRSA infections. Future studies proving the clinical significance of this combination therapy are therefore warranted.
Multiscale Parallel Algorithm for Early Detection of Tomato Gray Mold in a Complex Natural Environment
Authors: Wang Xuewei , Liu Jun
Published: May 2021

Plant disease detection technology is an important part of the intelligent agricultural Internet of Things monitoring system. The real natural environment requires the plant disease detection system to have extremely high real time detection and accuracy. The lightweight network MobileNetv2-YOLOv3 model can meet the real-time detection, but the accuracy is not enough to meet the actual needs. This study proposed a multiscale parallel algorithm MP-YOLOv3 based on the MobileNetv2-YOLOv3 model. The proposed method put forward a multiscale feature fusion method, and an efficient channel attention mechanism was introduced into the detection layer of the network to achieve feature enhancement. The parallel detection algorithm was used to effectively improve the detection performance of multiscale tomato gray mold lesions while ensuring the real-time performance of the algorithm. The experimental results show that the proposed algorithm can accurately and real-time detect multiscale tomato gray mold lesions in a real natural environment. The F1 score and the average precision reached 95.6 and 93.4% on the self-built tomato gray mold detection dataset. The model size was only 16.9 MB, and the detection time of each image was 0.022 s.
Cannabis Yield, Potency, and Leaf Photosynthesis Respond Differently to Increasing Light Levels in an Indoor Environment
Authors: Rodriguez-Morrison Victoria , Llewellyn David , Zheng Youbin
Published: May 2021

Since the recent legalization of medical and recreational use of cannabis (Cannabis sativa) in many regions worldwide, there has been high demand for research to improve yield and quality. With the paucity of scientific literature on the topic, this study investigated the relationships between light intensity (LI) and photosynthesis, inflorescence yield, and inflorescence quality of cannabis grown in an indoor environment. After growing vegetatively for 2 weeks under a canopy-level photosynthetic photon flux density (PPFD) of ≈425 μmol·m−2·s−1 and an 18-h light/6-h dark photoperiod, plants were grown for 12 weeks in a 12-h light/12-h dark “flowering” photoperiod under canopy-level PPFDs ranging from 120 to 1,800 μmol·m−2·s−1 provided by light emitting diodes. Leaf light response curves varied both with localized (i.e., leaf-level) PPFD and temporally, throughout the flowering cycle. Therefore, it was concluded that the leaf light response is not a reliable predictor of whole-plant responses to LI, particularly crop yield. This may be especially evident given that dry inflorescence yield increased linearly with increasing canopy-level PPFD up to 1,800 μmol·m−2·s−1, while leaf-level photosynthesis saturated well-below 1,800 μmol·m−2·s−1. The density of the apical inflorescence and harvest index also increased linearly with increasing LI, resulting in higher-quality marketable tissues and less superfluous tissue to dispose of. There were no LI treatment effects on cannabinoid potency, while there were minor LI treatment effects on terpene potency. Commercial cannabis growers can use these light response models to determine the optimum LI for their production environment to achieve the best economic return; balancing input costs with the commercial value of their cannabis products.
Dynamics of Maize Vegetative Growth and Drought Adaptability Using Image-Based Phenotyping Under Controlled Conditions
Authors: Dodig Dejan , Božinović Sofija , Nikolić Ana , Zorić Miroslav , Vančetović Jelena , Ignjatović-Micić Dragana , Delić Nenad , Weigelt-Fischer Kathleen , Altmann Thomas , Junker Astrid
Published: May 2021

Changes in climate are likely to have a negative impact on water availability and soil fertility in many maize-growing agricultural areas. The development of high-throughput phenotyping platforms provides a new prospect for dissecting the dynamic complex plant traits such as abiotic stress tolerance into simple components. The growth phenotypes of 20 maize (Zea mays L.) inbred lines were monitored in a non-invasive way under control, nitrogen, and water limitation as well as under combined nitrogen and water stress using an automated phenotyping system in greenhouse conditions. Thirteen biomass-related and morphophysiological traits were extracted from RGB images acquired at 33 time points covering developmental stages from leaf count 5 at the first imaging date to leaf count 10–13 at the final harvest. For these traits, genetic differences were identified and dynamic developmental trends during different maize growth stages were analyzed. The difference between control and water stress was detectable 3–10 days after the beginning of stress depending on the genotype, while the effect of limited nitrogen supply only induced subtle phenotypic effects. Phenotypic traits showed different response dynamics as well as multiple and changing interaction patterns with stress progression. The estimated biovolume, leaf area index, and color ratios were found to be stress-responsive at different stages of drought stress progression and thereby represent valuable reference indicators in the selection of drought-adaptive genotypes. Furthermore, genotypes could be grouped according to two typical growth dynamic patterns in water stress treatments by c-means clustering analysis. Inbred lines with high drought adaptability across time and development were identified and could serve as a basis for designing novel genotypes with desired, stage-specific growth phenotypes under water stress through pyramiding. Drought recovery potential may play an equal role as drought tolerance in plant drought adaptation.
Risk Factors for Delirium Are Different in the Very Old: A Comparative One-Year Prospective Cohort Study of 5,831 Patients
Authors: Marquetand Justus , Bode Leonie , Fuchs Simon , Hildenbrand Florian , Ernst Jutta , von Känel Roland , Boettger Soenke
Published: May 2021

Background: In an ever-aging society, health care systems will be confronted with an increasing number of patients over 80 years (“the very old”). Currently, knowledge about and recommendations for delirium management are often based on studies in patients aged 60 to 65 years. It is not clear whether these findings apply to patients ≥80 years.

Aim: Comparison of younger and older patients with delirium, especially regarding risk factors.

Methods: In this prospective cohort study, within 1-year, 5,831 patients (18–80 years: n = 4,730; ≥80: n = 1,101) with delirium were enrolled. The diagnosis of delirium was based on the Delirium Observation screening scale (DOS), Intensive Care Delirium Screening Checklist (ICDSC) and a DSM (Diagnostic and Statistical Manual)-5 construct of nursing instrument. Sociodemographic trajectories, as well as the relevant predisposing and precipitating factors for delirium, were assessed via a multiple regression analysis.

Results: The very old were more commonly admitted as emergencies (OR 1.42), had a greater mortality risk (OR 1.56) and displayed fewer precipitating risk factors for the development of a delirium, although the number of diagnoses were not different (p = 0.325). Predisposing factors were sufficient almost alone for the development of delirium in patients ≥ 80 years of age; in 18–80 years of age, additional precipitating factors had to occur to make a delirium possible.

Conclusion: When relevant predisposing factors for delirium are apparent, patients over 80 years of age require comparatively few or no precipitating factors to develop delirium. This finding should be taken into account at hospitalization and may allow better treatment of delirium in the future.
Excited Catatonia in Autism Spectrum Disorder: A Case Series
Authors: Burns Nora Kathleen , Grissett Kathleen , Macaluso Marc , Raza Mohsin , Gracious Barbara
Published: May 2021

Introduction: Autistic catatonia is an under-recognized debilitating syndrome with long-lasting negative effects for families, healthcare workers, and high-cost to the healthcare system. In this report, we describe two cases of excited catatonia in young men diagnosed with autism. Both endured a delay to diagnosis and difficulty to obtain appropriate treatment.

Main concern: Each patient had a change in behavior from their baseline but with differences in severity and onset. The diagnosis in the first patient was made after only 3 months as the change was dramatic and sudden. Yet, despite a confirmed diagnosis, it was difficult to treat as the importance of M-ECT was not recognized by the clinicians. The second patient had been suffering for more than 5 years with a slow progression of worsening aggressive symptoms. The aggression was so uncontrollable that the patient required sedation, intubation and daily ECT. Both suffered from agitation, unprovoked aggression, urinary incontinence, stereotypic, and OCD behaviors and compulsive masturbation.

Primary Diagnosis, intervention/outcomes: Both patients were diagnosed with autism, one high-functioning, attending high school and working a part-time job, the second low-functioning, nearly non-verbal, isolated to home and ABA school. The first patient's diagnosis of catatonia was only suspected after five psychiatric admissions and more than 20 medication trials. Lorazepam challenge was effective, he was treated with a short series of ECT but each time the treatments were tapered, the aggression returned. Ultimately, stabilized on weekly ECT. The second patient's behavior was escalating over a 5 month period, to the point, the aggression was uncontrollable. He presented to the ED under involuntary hold and the behavior could not be stabilized to the point that emergent ECT was initiated.

Conclusion: Two cases of autistic catatonia diagnosed and treated within a year time span at a small community hospital indicates that this diagnosis is more common than previously recognized. We propose screening all patients with neurodevelopmental disorders with the Bush-Francis and Kanner scales to diagnose and treat them appropriately.
The implementation of the Student Response System (SRS) Kahoot! in EFL classes and its effects on reading comprehension
Authors: Narváez Paguay Joe Adrián , Santillán Juan José
Published: May 2021

Improvement of teacher-student class interaction has been obtaining greater progresses thanks to technological development with the addition of practical resources and modern facilities in educational institutions. The use of game-based response systems for enhancing language skills inside an EFL classroom has reported benefits mainly for certain particular skills like grammar and sentence structuring.  In this study, the online gaming platform Kahoot! was used specifically for enhancing the reading comprehension skill through a period of nearly three months on students of A2 level from Universidad de Cuenca in Ecuador. The intervention of the Kahoot! website focused on the comprehension and remembering of specific details by means of quizzes. The research design of this study used a mixed-method based on the Hypothetico-Deductive approach to research and data collection instruments, as well as their further analysis and interpretation. Pre and posttests were used in order to compare the results before and after the methodology. The outcomes revealed a statistical improvement with favorable effects regarding the pre and posttests and also a general learner acceptance towards the stated methodology. Further research is suggested on the use of Kahoot! for reading comprehension improvement purposes and its continuous use as a supporting tool for remembering specific details of texts, together with other subskills which could possibly be benefitted from this methodology in the field of EFL instruction.
Akademickie periodyki narodowe. Z dziejów prasy Narodowej Demokracji (do 1939 roku
Authors: Dawidowicz Aneta
Published: May 2021

The community periodicals had accompanied the creation process of the press system in the Polish territory since the end of the 19th century. The community dimension of the press relates to both its spatial scope and the concreteness of the publishing profile. The National Democracy press was a collection of periodicals characterised by their typological diversity, in which the world presented equalled reality of the readers. From its beginnings, the National Democracy treated press in a purely utilitarian manner, as a form of dissemination of political thought and the tool which supported the achievement of political goals. The press took a multifaceted part in the development of national democratic movement.
Escala de satisfacción con el tratamiento recibido (cres-4): la versión en español
Authors: Feixas Guillem , Pucurull Olga , Roca C. , Paz Clara , García-Grau Eugeni , Bados Arturo
Published: May 2021

La satisfacción de consumidores y usuarios con los productos y servicios es un tema que en los últimos años ha ido adquiriendo cada vez más importancia hasta alcanzar también el campo de la psicoterapia. En este trabajo se presenta la adaptación al español de la Escala de Satisfacción con el Tratamiento Recibido (CRES-4). Tiene cuatro ítems y fue creada para evaluar el grado de satisfacción del cliente con la terapia recibida, el grado en que considera que su problema principal se ha resuelto y el cambio percibido en su estado emocional del pretratamiento al postratamiento. La puntuación global pretende reflejar la eficacia del tratamiento según el paciente (Nielsen et al., 2004). Los estudios realizados hasta la fecha con este instrumento sugieren que la CRES-4 puede ser un buen instrumento complementario, especialmente si se quiere evaluar la satisfacción con la psicoterapia.
El narcisismo y sus modalidades
Authors: Villegas Manuel , Mallor Pilar
Published: May 2021

En este artículo se presenta una visión constructivista del narcisismo, concebido como un déficit de descentramiento en el proceso de diferenciación del yo. La función asimilativa del egocentrismo se considera la causa de la ausencia de empatía típica del narcisismo. Las modalidades que puede adoptar el narcisismo se subdividen en tres tipos denominados aristocrático, meritocrático y plutocrático.
A Modified Dynamic Programming Approach for 4D Minimum Fuel and Emissions Trajectory Optimization
Authors: Ahmed Kawser , Bousson Kouamana , Coelho Milca de Freitas
Published: May 2021

4D flight trajectory optimization is an essential component to improve flight efficiency and to enhance air traffic capacity. this technique not only helps to reduce the operational costs, but also helps to reduce the environmental impact caused by the airliners. This study considers Dynamic Programming (DP), a well-established numerical method ideally suited to solve 4D flight Trajectory Optimization Problems (TOPs). However, it bears some shortcomings that prevent the use of DP in many practical real-time implementations. This paper proposes a Modified Dynamic Programming (MDP) approach that reduces the computational effort and overcomes the drawbacks of the traditional DP. In this paper, two numerical examples with fixed arrival times are presented, where the proposed MDP approach is successfully implemented to generate optimal trajectories that minimize aircraft fuel consumption and emissions. Then the obtained optimal trajectories are compared with the corresponding reference commercial flight trajectory for the same route in order to quantify the potential benefit of reduction of aircraft fuel consumption and emissions.
Weed Species Trait Selection as Shaped by Region and Crop Diversity in Organically Managed Spring Cereals
Authors: Hofmeijer Merel A. J. , Melander Bo , Salonen Jukka , Lundkvist Anneli , Zarina Livija , Gerowitt Bärbel
Published: May 2021

Weeds remain a challenge in organic arable farming, as well as supply ecosystem services. The aim is to control weed densities while hosting a diverse and manageable weed community, preventing domination of few deleterious species. Therefore, we want to understand how specific species are stimulated, and which traits are selected for. This study focuses on crop diversity hypothesizing that (1) regions and (2) crop diversity function as filters for specific weed species traits. We conducted a weed monitoring in spring cereals over 2 years on organic farms in five northern European regions. Management and weed trait variables collected for the occurring species allowed an RLQ fourth-corner analysis. The weed communities were regionally specific, but trait selection was not observed, except in Latvia. Hence, the regional species pool provided different species with similar traits. Crop diversity within the management of spring cereals, such as undersowing and cereal frequency in the rotation, affected weed traits. The number of years under organic production selected no traits, although species numbers are known to increase. Hence, general weed species diversity increased, irrespective of traits. We conclude that organic management may support the agility within the weed community against selection of species and act as a buffer rather than as filter.
Effects of Inorganic Fertilizers on Virulence of the Entomopathogenic Nematode Steinernema glaseri and Peanut Germination under Field Conditions
Authors: Shehata Ibrahim E. , Hammam Mostafa M. A. , Abd-Elgawad Mahfouz M. M.
Published: May 2021

The use of entomopathogenic nematodes as safe biopesticidal alternatives to hazardous chemicals entails improving the prediction of their native efficacy against soil pests. The effect of ten inorganic fertilizers, used extensively in Egypt, on the virulence of indigenous Steinernema glaseri and peanut germination was examined herein. The nematode added either before or tank-mixed with 1%, 5%, and 10% concentrations of each fertilizer in a peanut field was sampled 1 and 7 days before and 1, 7, 14, 21, 28, 49, and 56 post-tank mixes to check for S. glaseri virulence via baiting soil with Galleria mellonella larvae. Phosphorus fertilizers had more adverse effects than others on S. glaseri virulence and peanut germination. Plots with only S. glaseri had high germination close to chlorpyrifos. Averages of insect mortality in soil samples of potassium, nitrogen: phosphorus: potassium (NPK), nitrogenous, and phosphorus fertilizers, and non-fertilized checks (nematode only) were 85.8, 83.8, 80, 69.2%, and 93.3% respectively. Using S. glaseri is preferred before fertilizing. Most 1% fertilizer concentrations are compatible with S. glaseri in tank mixes for short-term (1–7 days) insect control but may affect long-term control.
Studying the Genetic Diversity of Yam Bean Using a New Draft Genome Assembly
Authors: Tay Fernandez Cassandria G. , Pati Kalidas , Severn-Ellis Anita A. , Batley Jacqueline , Edwards David
Published: May 2021

Yam bean (Pachyrhizus erosus Rich. Ex DC.) is an underutilized leguminous crop which has been used as a food source across central America and Asia. It is adapted to a range of environments and is closely related to major leguminous food crops, offering the potential to understand the genetic basis of environmental adaptation, and it may be used as a source of novel genes and alleles for the improvement of other legumes. Here, we assembled a draft genome of P. erosus of 460 Mbp in size containing 37,886 gene models. We used this assembly to compare three cultivars each of P. erosus and the closely related P. tuberosus and identified 10,187,899 candidate single nucleotide polymorphisms (SNPs). The SNP distribution reflects the geographic origin and morphology of the individuals.
Forecast of Changes in Air Temperatures and Heat Indices in the Sevastopol Region in the 21st Century and Their Impacts on Viticulture
Authors: Vyshkvarkova Elena , Rybalko Evgeniy
Published: May 2021

Climate is a limiting factor in viticulture, as it defines favorable areas, grape cultivars, and agrotechnical activities. In the Sevastopol region, viticulture is the main and promising agricultural branch. Using the outputs of the regional climate models from the CORDEX project, the projections of agroclimatic conditions in the Sevastopol region for two future periods (2021–2045 and 2046–2070) under two representative concentration pathways (RCP4.5 and RCP8.5) were obtained. The results in our study show the trend of temperature indices rise (average growing season temperature, effective heat sum, Winkler and Huglin indices) and the region’s transition to higher classes, especially during the second future period (2046–2070). However, despite the higher temperature indices, the Sevastopol region will remain suitable for the growing of grapes cultivars with all ripening periods.
Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity
Authors: Yeo Dahee , Hwang Su-Jung , Song Ye-Seul , Lee Hyo-Jong
Published: May 2021

This study was designed to determine whether α-humulene, a major constituent in many plants used in fragrances, has a protective role against gastric injury in vivo and in vitro. A rat model of hydrochloric acid (HCl)/ethanol-induced gastritis and human mast cells (HMC-1) were used to investigate the mucosal protective effect of α-humulene. α-Humulene significantly inhibited gastric lesions in HCl/ethanol-induced acute gastritis and decreased gastric acid secretion pyloric ligation-induced gastric ulcers in vivo. In addition, α-humulene reduced the amount of reactive oxygen species and malondialdehyde through upregulation of prostaglandin E2 (PGE2) and superoxide dismutase (SOD). In HMC-1 cells, α-humulene decreased intracellular calcium and increased intracellular cyclic adenosine monophosphate (cAMP) levels, resulting in low histamine levels. α-Humulene also reduced the expression levels of cytokine genes such as interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF) by downregulating nuclear factor-κB (NF-κB) nuclear translocation. Finally, α-humulene upregulated the expression levels of mucin 5AC (Muc5ac), Muc6, trefoil factor 1 (Tff1), trefoil factor 2 (Tff2), and polymeric immunoglobulin receptor (pigr). α-Humulene may attenuate HCl/ethanol-induced gastritis by inhibiting histamine release and NF-κB activation and stimulating antioxidants and mucosal protective factors, particularly Muc5ac and Muc6. Therefore, these data suggest that α-humulene is a potential drug candidate for the treatment of stress-induced or alcoholic gastritis.
Direct Calculation of the Group Velocity for Two-Dimensional Complex, Composite and Periodic Structures Using a Wave and Finite Element Scheme
Authors: Malik Muhammad Khalid , Chronopoulos Dimitrios , Ciampa Francesco
Published: May 2021

Guided waves have immense potential for structural health monitoring applications in numerous industries including aerospace. It is necessary to evaluate guided wave dispersion characteristics, i.e., group velocity and phase velocity profiles, for using them effectively. For complex structures, the profiles can have highly irregular shapes. In this work, a direct method for calculating the group velocity profiles for complex, composite, and periodic structures using a wave and finite element scheme is presented. The group velocity calculation technique is easy to implement, highly computationally efficient, and works with the standard finite element formulation. The major contribution is summarised in the form of a comprehensive algorithm for calculating the group velocity profiles. The method is compared with the existing analytical and numerical methods for calculation of dispersion curves. Finally, an experimental study in a multilayered composite plate is conducted and the results are found to be in good agreement. The technique is suitable to be used in all guided wave application areas such as material characterisation, non-destructive testing, and structural health monitoring.
Community Composition and Ex Situ Cultivation of Fungi Associated with UNESCO Heritage Monuments in the Bay of Naples
Authors: Petraretti Mariagioia , Duffy Karl J. , Del Mondo Angelo , Pollio Antonino , De Natale Antonino
Published: May 2021

The Bay of Naples, Italy, is renowned for its archaeological heritage. However, this heritage is threatened by the combination of weathering and the biological activity of microorganisms. Fungi are among the major agents of microbial deterioration of cultural heritage since they can cause cracks and lesions in monuments due to the penetrating force of their hyphae. Such biodeterioration may weaken the stone structures and threaten the longevity of these culturally important monuments. To address this, we collected, identified, and maintained in culture filamentous fungi that colonize the external surface of monuments at five important archaeological sites near Naples, namely Cuma, Ercolano, Nola, Oplonti, and Pompei. We isolated a total of 27 fungal taxa, all of which can be cultivated in the laboratory, and form a part of our reference collection. Many of the described fungal taxa we found belong to groups that are involved in stone biodeterioration and can thus be considered as model organisms for in vitro studies. These results emphasize the importance of identifying and cultivating fungal stock cultures for non-invasive studies on biodeterioration. Our newly developed reference collection represents a useful resource that is available to other researchers to rapidly identify potentially hazardous fungi on other monuments.