bluuebunny/crossref_metadata_2025 huggingface dataset:
Unintended Consequences of Opioid Regulations in Older Adults with Multiple Chronic Conditions
Authors: Ritchie Christine S , Garrett Sarah B , Thompson Nicole , Miaskowski Christine
Published: January 2020

Background and Objectives

The opioid epidemic has led to substantive regulatory and policy changes. Little is known about how these changes have impacted older adults, especially those with chronic pain and multiple chronic conditions (MCC). We sought to understand the experiences of older adults with chronic pain and MCC in the context of the opioid epidemic and policy responses to it.

Research Design and Methods

Purposive sampling of older adults in a West Coast metropolitan area. Semistructured in-depth interviews lasting 45–120 min were digitally recorded and transcribed. Responses were analyzed using the constant comparative method. Participants were 25 adults aged 65 years and greater with three or more self-reported medical conditions and pain lasting for more than 6 months.

Results

Respondents’ accounts revealed numerous unintended consequences of the opioid epidemic and its policy responses. We identified four main themes: changes to the patient–clinician relationship; lack of patient agency and access in pain management; patient ambivalence and anxiety about existing opioid treatment/use; and patient concerns about future use.

Discussion and Implications

Older adults have high rates of chronic pain and MCC that may reduce their pain management options. The opioid epidemic and policies addressing it have the potential to negatively affect patient–clinician relationships and patients’ pain self-management. Clinicians may be able to mitigate these unintended consequences by actively conveying respect to the patient, empowering patients in their pain self-management activities, and proactively addressing worries and fears patients may own related to their current and future pain management regimens.
Three-station interferometry and tomography: coda versus direct waves
Authors: Zhang Shane , Feng Lili , Ritzwoller Michael H
Published: January 2020

Traditional two-station ambient noise interferometry estimates the Green’s function between a pair of synchronously deployed seismic stations. Three-station interferometry considers records observed three stations at a time, where two of the stations are considered receiver–stations and the third is a source–station. Cross-correlations between records at the source–station with each of the receiver–stations are correlated or convolved again to estimate the Green’s function between the receiver–stations, which may be deployed asynchronously. We use data from the EarthScope USArray in the western United States to compare Rayleigh wave dispersion obtained from two-station and three-station interferometry. Three three-station interferometric methods are distinguished by the data segment utilized (coda-wave or direct-wave) and whether the source–stations are constrained to lie in stationary phase zones approximately inline with the receiver–stations. The primary finding is that the three-station direct wave methods perform considerably better than the three-station coda-wave method and two-station ambient noise interferometry for obtaining surface wave dispersion measurements in terms of signal-to-noise ratio, bandwidth, and the number of measurements obtained, but possess small biases relative to two-station interferometry. We present a ray-theoretic correction method that largely removes the bias below 40 s period and reduces it at longer periods. Three-station direct-wave interferometry provides substantial value for imaging the crust and uppermost mantle, and its ability to bridge asynchronously deployed stations may impact the design of seismic networks in the future.
Seismic characterization of a clay-block rupture in Harmalière landslide, French Western Alps
Authors: Fiolleau S , Jongmans D , Bièvre G , Chambon G , Baillet L , Vial B
Published: January 2020

In late June 2016, the Harmalière clayey landslide (located 30 km south of the city of Grenoble, French Alps) was dramatically reactivated at the headscarp after a 35-yr-long period of continuous but limited activity. The total involved volume, which moved as sliding blocks of various sizes, was estimated to be about 2 × 10 6 m3. Two seismometers were installed at the rear of the main headscarp in August 2016, on both sides of a developing fracture delineating a block with a volume of a few hundred cubic metres. For 4 months, they continuously recorded seismic ambient vibrations and microearthquakes until the block broke. Five seismic parameters were derived from the monitoring: the cumulative number of microearthquakes (CNe), the seismic energy (SE), the block resonance frequency (fB), the relative variation in Rayleigh wave velocity (dV/V) deduced from noise cross-correlations between the two sensors and the associated correlation coefficient (CC). All parameters showed a significant precursory signal before the rupture, but at very different times, which indicates the complexity of the rupture mechanism in this clay material.
Wartime paradigms and the future of western military power
Authors: Schmitt Olivier
Published: January 2020

From the perception of the imminence of threats at the political level to the seizing of initiative through proper timing at the tactical level, temporality is directly related to war and warfare. Yet, despite some analyses of the importance of time at the political/grand strategic level (usually by scholars) and at the tactical level (usually by military professionals) there is surprisingly little discussion of the impact of time on the preparation and the conduct of warfare. This article introduces the concept of ‘wartime paradigm’ as a heuristic device to understand the relationship between the perception of time and the conduct of warfare, and argues that after the Cold War, a specific ‘wartime paradigm’ combining an optimization for speed and an understanding of war as risk management has guided western warfare, from force structure to the conduct of actual operations. It shows how the changing character of warfare directly challenges this wartime paradigm and why, if western forces want to prevail in future conflicts, the establishment of a new wartime paradigm guiding technological improvements and operational concepts is critical.
The juche H-bomb? North Korea, nuclear weapons and regime-state survival
Authors: Howell Edward
Published: January 2020

Existing scholarship on North Korea's nuclear programme remains overwhelmingly centred around questions of containment or engagement with the North Korean regime-state, amid international calls for denuclearization. Yet, scholarship has rarely interrogated the precise value of nuclear weapons to the regime-state. This article develops a new theoretical framework of nuclear ideology to explore the puzzle of the survival of North Korea. This framework aims to show how the North Korean nuclear programme is deeply entrenched within the state ideology of juche, as one device for continued regime-state survival. Through interviews with elite North Korean defectors and textual analysis of North Korean and international sources, I show that North Korea's nuclear ideology has been constructed according to different frames of meaning, targeting referent actors of international ‘enemy’ powers and domestic audiences. This article concludes that nuclear ideology functions primarily as a tool to arouse domestic legitimacy for the North Korean regime-state, by targeting elite actors within the highly stratified domestic population. From an international perspective, perception of North Korea's survival remains tied largely to the regime-state's physical possession of nuclear weapons. This article has extremely timely theoretical and policy implications given the current ‘dialogue’ between US and North Korean leaders. First, it opens up fruitful avenues of inquiry surrounding questions of the legitimacy of rogue states within international relations. Secondly, this article calls for a more robust understanding of the domestic-level politics of North Korea, in order to understand the regime-state's foreign policy decisions vis-à-vis its nuclear programme.
Elliptic Curves in Hyper-Kähler Varieties
Authors: Nesterov Denis , Oberdieck Georg
Published: January 2020

We show that the moduli space of elliptic curves of minimal degree in a general Fano variety of lines of a cubic four-fold is a non-singular curve of genus $631$. The curve admits a natural involution with connected quotient. We find that the general Fano contains precisely $3,780$ elliptic curves of minimal degree with fixed (general) $j$-invariant. More generally, we express (modulo a transversality result) the enumerative count of elliptic curves of minimal degree in hyper-Kähler varieties with fixed $j$-invariant in terms of Gromov–Witten invariants. In $K3^{[2]}$-type this leads to explicit formulas of these counts in terms of modular forms.
Rapid detection of plasmid-mediated high-level tigecycline resistance in Escherichia coli and Acinetobacter spp
Authors: Cui Ze-Hua , Ni Wei-Na , Tang Tian , He Bing , Zhong Zi-Xing , Fang Liang-Xing , Chen Liang , Chen Chong , Cui Chao-Yue , Liu Ya-Hong , Liao Xiao-Ping , Sun Jian
Published: January 2020

Objectives

The emergence and spread of plasmid-encoded tet(X3/X4) genes that confer high-level tigecycline and eravacycline resistance in Escherichia coli and Acinetobacter spp. pose serious threats to human and animal health. We developed a rapid and robust assay to detect Tet(X3/X4) in Gram-negative bacteria based on eravacycline degradation by the presence of the Tet(X) enzyme in the test strain.

Methods

This tetracycline inactivation method (TIM) is based on the degradation of eravacycline by the Tet(X3/X4)-producing strain, which results in reduced eravacycline activity against an acid-producing thermophile Bacillus stearothermophilus indicator strain. For Tet(X)-negative strains, eravacycline retains its antimicrobial activity. Coupled with a pH-sensitive dye (bromocresol purple), the reduced colorimetric inhibition zone can be measured to determine the production of Tet(X3/X4). One hundred and eighteen isolates, including 30 tet(X4)-positive E. coli, 30 tet(X3)-positive Acinetobacter spp. and 58 tet(X)-negative E. coli and Acinetobacter spp., were examined to evaluate the performance of this TIM.

Results

The sensitivity and specificity for E. coli carrying tet(X4) was 96.7% and 100%, respectively, and for Acinetobacter spp. carrying tet(X3) both were 100%. The TIM assay can be completed within 6.5 h.

Conclusions

The TIM is a simple, rapid and cost-effective method for the detection of plasmid-mediated high-level tigecycline resistance in E. coli and Acinetobacter spp.
Proposed Plasma Ammonia Reference Intervals in a Reference Group of Hospitalized Term and Preterm Neonates
Authors: Madigan Theresa , Block Darci R , Carey William A , Kaemingk Bethany D , Patel Robin
Published: January 2020

Background

Plasma ammonia is commonly measured in the diagnostic evaluation of hospitalized newborns, but reference values are not well defined.

Methods

We prospectively enrolled newborns admitted to the level III/IV neonatal intensive care unit and level II intermediate special care nursery from January 2017 to January 2018. Infants with inborn errors of metabolism or liver disease were excluded. Plasma ammonia concentrations were measured once within the first week of life and evaluated by sex, gestational age, timing of the draw, blood collection method, and type of nutrition. Reference intervals were calculated.

Results

127 neonates were included; one third (34%) were term infants born at ≥37 weeks gestation, and two thirds (66%) were born preterm at <37 weeks gestation. Median plasma ammonia concentrations were 32 μmol/L (range <10 to 86 μmol/L). Median ammonia concentrations were higher among preterm compared to term infants (35 vs. 28 μmol/L, p = 0.0119), and term female compared to term male infants (34 vs. 26 μmol/L, p = 0.0228). There was no difference in median ammonia concentrations between female and male preterm infants, based on gestational age within the preterm group, timing of the blood draw, presence of hyperbilirubinemia, blood collection method, or type of nutritional intake.

Conclusions

Plasma ammonia concentrations among newborns are higher than the expected adult concentrations and may vary by gestational age and sex. Blood collection method, type of nutrition, hyperbilirubinemia, and timing of the draw do not impact concentrations. We propose a reference limit of ≤82 μmol/L for newborns less than one week of age.
Chemical Comparison of Ophiopogonis radix and Liriopes radix Based on Quantitative Analysis of Multiple Components by HPLC Coupled with Electrospray Ionization Tandem Triple Quadrupole Mass Spectrometry
Authors: Luo Heng , Ming Liang-Shan , Tong Tian-Tian , Tang Yan , Yang Jun , Shen Li , Cui Hongmei , Yang Andong , Huang Hao
Published: January 2020

Background

Ophiopogonis radix and Liriopes radix are well known for the treatment of dry coughs and phthisis. Liriopes radix is occasionally used as a substitute for Ophiopogonis radix in various prescriptions due to the extremely similar pharmacological activities and clinical efficacies, but they are regarded as two different remedies in the Chinese Pharmacopoeia. Accordingly, the establishment of a reliable analytical approach for the discrimination and quality evaluation of Ophiopogonis and Liriopes is required.

Objective

To establish a simple, accurate, and reliable method that can simultaneously determine multiple components in Ophiopogonis radix and Liriopes radix. To comprehensively compare the chemical compositions of the two herbs and find markers for discrimination and quality assessments.

Method

An HPLC-ESI-triple quadrupole (QQQ)-MS/MS method was developed for simultaneous characterization and quantification of chemical components in the two herbs. The results were further analyzed by PLS discriminant analysis to provide more information about the chemical differences, as well as to evaluate the quality of each sample.

Results

A total of 23 compounds have been characterized and quantified in 31 batches of herbs from different geographical regions, among which liriopesides B, sprengerinin A, ophiopogonin B, and ophiopogonanone E contribute mostly. The contents of homoisoflavonoids were much higher in Ophiopogonis radix than in Liriopes radix, but the levels of steroidal saponins followed a contrary trend.

Conclusions

Simultaneous determination of multiple components by HPLC-QQQ-MS/MS coupled with chemometrics analysis is an acceptable strategy to evaluate and control the quality of Ophiopogonis radix and Liriope radix.

Highlights

Simultaneous determination of 12 steroidal saponins and 11 homoisoflavonoids in both Ophiopogonis radix and Liriope radix by using HPLC-QQQ-MS/MS in positive ion mode, as well as the quality control study.
Parents’ Perspectives on Their Child’s Music Therapy: A Qualitative Study
Authors: Annesley Luke , Curtis-Tyler Katherine , McKeown Eamonn
Published: January 2020

This qualitative study explored parents’ perspectives on their child receiving individual music therapy in a community setting in an NHS service in London, UK. Parents of children aged 6–11 receiving or recently discharged from music therapy took part. Data were collected through semi-structured interviews which were digitally recorded, transcribed, and analyzed following procedures of inductive thematic analysis. Music therapy was generally perceived to provide a nurturing environment for children, and communication with music therapists was mostly experienced as helpful, but with some perceived challenges. Parents perceived positive outcomes of music therapy, including children becoming calmer and engaging more with musical activities at home. Understanding of music therapy was perceived as an evolving process for parents. Some parents described a need for more information prior to music therapy. The findings of this study broadly support the model in the service of working with children aged 6–11 without parents generally present during sessions. However, there were indications that, for some families, a more flexible approach might be beneficial for the child. Participants’ commentaries on perceived outcomes for children indicate potential for intervention studies investigating the impact of music therapy, while broader perceptions of the value of music therapy indicate a need for studies exploring reasons for effectiveness.
Duplication and functional divergence of a calcium sensor in the Brassicaceae
Authors: Monihan Shea M , Magness Courtney A , Ryu Choong-Hwan , McMahon Michelle M , Beilstein Mark A , Schumaker Karen S
Published: January 2020

The presence of varied numbers of CALCINEURIN B-LIKE10 (CBL10) calcium sensor genes in species across the Brassicaceae and the demonstrated role of CBL10 in salt tolerance in Arabidopsis thaliana and Eutrema salsugineum provided a unique opportunity to determine if CBL10 function is modified in different species and linked to salt tolerance. Salinity effects on species growth and cross-species complementation were used to determine the extent of conservation and divergence of CBL10 function in four species representing major lineages within the core Brassicaceae (A. thaliana, E. salsugineum, Schrenkiella parvula, and Sisymbrium irio) as well as the first diverging lineage (Aethionema arabicum). Evolutionary and functional analyses indicate that CBL10 duplicated within expanded lineage II of the Brassicaceae and that, while portions of CBL10 function are conserved across the family, there are species-specific variations in CBL10 function. Paralogous CBL10 genes within a species diverged in expression and function probably contributing to the maintenance of the duplicated gene pairs. Orthologous CBL10 genes diverged in function in a species-specific manner, suggesting that functions arose post-speciation. Multiple CBL10 genes and their functional divergence may have expanded calcium-mediated signaling responses and contributed to the ability of certain members of the Brassicaceae to maintain growth in salt-affected soils.
Antifungal activity of promethazine and chlorpromazine against planktonic cells and biofilms of Cryptococcus neoformans/Cryptococcus gattii complex species
Authors: Brilhante Raimunda Sâmia Nogueira , Gotay Wilker Jose Perez , Pereira Vandbergue Santos , de Oliveira Jonathas Sales , Pereira-Neto Waldemiro Aquino , Castelo-Branco Débora de Souza Collares Maia , Cordeiro Rossana de Aguiar , Sidrim José Júlio Costa , Rocha Marcos Fábio Gadelha
Published: January 2020

Cryptococcus neoformans/Cryptococcus gattii are fungal pathogens that affect the central nervous system, mainly in immunocompromised individuals. Due to the limited pharmacological arsenal available for the treatment of cryptococcosis associated with cases of antifungal resistance of Cryptococcus spp. reported in some studies, the search for new compounds with antifungal potential becomes relevant. Thus, the objective of this study was to evaluate the inhibitory effect of phenothiazines (promethazine and chlorpromazine) on C. neoformans/C. gattii planktonic cells and biofilms. In vitro planktonic susceptibility testing was performed using the broth microdilution assay. The effect of phenothiazines was evaluated against biofilm formation and mature Cryptococcus biofilms. Biofilm morphology and ultrastructure were also evaluated by scanning electron microscopy. Promethazine and chlorpromazine showed antifungal activity against planktonic cells, with minimum inhibitory concentrations of 8–32 μg/ml and 4–16 μg/ml, respectively. As for biofilm formation, phenothiazines reduced biomass by 60% and metabolic activity by 90% at 64 μg/ml; while in mature biofilms, reductions of 85% and 90% in biomass and metabolic activity, respectively, were observed at 1024 μg/ml. Promethazine and chlorpromazine were also able to disrupt and fragment biofilms. In conclusion, promethazine and chlorpromazine have antifungal activity against planktonic cells and biofilms of Cryptococcus spp. These data show the potential of promethazine and chlorpromazine as antibiofilm drugs.
Redshift evolution of the Fundamental Plane relation in the IllustrisTNG simulation
Authors: Lu Shengdong , Xu Dandan , Wang Yunchong , Mao Shude , Ge Junqiang , Springel Volker , Wang Yuan , Vogelsberger Mark , Naiman Jill , Hernquist Lars
Published: January 2020

We investigate the Fundamental Plane (FP) evolution of early-type galaxies in the IllustrisTNG-100 simulation (TNG100) from redshift z = 0 to z = 2. We find that a tight plane relation already exists as early as z = 2. Its scatter stays as low as ∼0.08 dex across this redshift range. Both slope parameters b and c (where R ∝ σbIc with R, σ, and I being the typical size, velocity dispersion, and surface brightness) of the plane evolve mildly since z = 2, roughly consistent with observations. The FP residual $\rm Res$ ($\equiv \, a\, +\, b\log \sigma \, +\, c\log I\, -\, \log R$, where a is the zero-point of the FP) is found to strongly correlate with stellar age, indicating that stellar age can be used as a crucial fourth parameter of the FP. However, we find that 4c + b + 2 = δ, where δ ∼ 0.8 for FPs in TNG, rather than zero as is typically inferred from observations. This implies that a tight power-law relation between the dynamical mass-to-light ratio Mdyn/L and the dynamical mass Mdyn (where Mdyn ≡ 5σ2R/G, with G being the gravitational constant) is not present in the TNG100 simulation. Recovering such a relation requires proper mixing between dark matter and baryons, as well as star formation occurring with correct efficiencies at the right mass scales. This represents a powerful constraint on the numerical models, which has to be satisfied in future hydrodynamical simulations.
Are the carriers of diffuse interstellar bands and extended red emission the same?
Authors: Lai Thomas S-Y , Witt Adolf N , Alvarez Carlos , Cami Jan
Published: January 2020

We report the first spectroscopic observations of a background star seen through the region between the ionization front and the dissociation front of the nebula IC 63. This photodissociation region (PDR) exhibits intense extended red emission (ERE) attributed to fluorescence by large molecules/ions. We detected strong diffuse interstellar bands (DIB) in the stellar spectrum, including an exceptionally strong and broad DIB at λ4428. The detection of strong DIBs in association with ERE could be consistent with the suggestion that the carriers of DIBs and ERE are identical. The likely ERE process is recurrent fluorescence, enabled by inverse internal conversions from highly excited vibrational levels of the ground state to low-lying electronic states with subsequent transitions to ground. This provides a path to rapid radiative cooling for molecules/molecular ions, greatly enhancing their ability to survive in a strongly irradiated environment. The ratio of the equivalent widths (EWs) of DIBs λ5797 and λ5780 in IC 63 is the same as that observed in the low-density interstellar medium with ultraviolet (UV) interstellar radiation fields (ISRF) weaker by at least two orders of magnitude. This falsifies suggestions that the ratio of these two DIBs can serve as a measure of the UV strength of the ISRF. Observations of the nebular spectrum of the PDR of IC 63 at locations immediately adjacent to where DIBs were detected failed to reveal any presence of sharp emission features seen in the spectrum of the Red Rectangle nebula. This casts doubts upon proposals that the carriers of these features are the same as those of DIBs seen at slightly shorter wavelengths.
A SCUBA-2 850 μm survey of heavily reddened quasars at z ∼ 2
Authors: Wethers Clare F , Banerji Manda , Hewett Paul C , Jones Gareth C
Published: January 2020

We present new 850 μm SCUBA-2 observations for a sample of 19 heavily reddened Type-I quasars at redshifts z ∼ 2 with dust extinctions of AV ≃ 2–6 mag. Three of the 19 quasars are detected at >3σ significance corresponding to an 850 μm flux-limit of ≳4.8 mJy. Assuming the 850 μm flux is dominated by dust heating due to star formation, very high star formation rates (SFR) of ∼2500–4500 M⊙ yr−1 in the quasar host galaxies are inferred. Even when considering a large contribution to the 850 μm flux from dust heated by the quasar itself, significant SFRs of ∼600–1500 M⊙ yr−1 are nevertheless inferred for two of the three detected quasars. We stack the remaining 16 heavily reddened quasars and derive an average 3σ upper limit on the SFRs in these quasar host galaxies of <880 M⊙ yr−1. The number counts of sub-mm galaxies in the total survey area (134.3 arcmin2) are consistent with predictions from blank-field surveys. There are, however, individual quasars where we find evidence for an excess of associated sub-mm galaxies. For two quasars, higher spatial resolution and spectroscopic ALMA observations confirm the presence of an excess of sub-mm sources. We compare the 850 μm detection rate of our quasars to both unobscured, ultraviolet luminous quasars as well as the much more obscured population of mid-infrared luminous Hot Dust Obscured Galaxies (HotDOGs). When matched by luminosity and redshift, we find no significant differences in the 850 μm flux densities of these various quasar populations given the current small sample sizes.
Simulations and symmetries
Authors: Modi Chirag , Chen Shi-Fan , White Martin
Published: January 2020

We investigate the range of applicability of a model for the real-space power spectrum based on N-body dynamics and a (quadratic) Lagrangian bias expansion. This combination uses the highly accurate particle displacements that can be efficiently achieved by modern N-body methods with a symmetries-based bias expansion which describes the clustering of any tracer on large scales. We show that at low redshifts, and for moderately biased tracers, the substitution of N-body-determined dynamics improves over an equivalent model using perturbation theory by more than a factor of two in scale, while at high redshifts and for highly biased tracers the gains are more modest. This hybrid approach lends itself well to emulation. By removing the need to identify haloes and subhaloes, and by not requiring any galaxy-formation-related parameters to be included, the emulation task is significantly simplified at the cost of modelling a more limited range in scale.
A multiwavelength study of spiral structure in galaxies. I. General characteristics in the optical
Authors: Savchenko Sergey , Marchuk Alexander , Mosenkov Aleksandr , Grishunin Konstantin
Published: January 2020

Different spiral generation mechanisms are expected to produce different morphological and kinematic features. In this first paper in a series, we carefully study the parameters of spiral structure in 155 face-on spiral galaxies, selected from the Sloan Digital Sky Survey, in the three gri bands. We use a method for deriving a set of parameters of spiral structure, such as the width of the spiral arms, their fraction to the total galaxy luminosity, and their colour, which have not been properly studied before. Our method is based on an analysis of a set of photometric cuts perpendicular to the direction of a spiral arm. Based on the results of our study, we compare the main three classes of spirals: grand design, multi-armed, and flocculent. We conclude that: (i) for the vast majority of galaxies (86 per cent), we observe an increase of their arm width with Galactocentric distance; (ii) more luminous spirals in grand design galaxies exhibit smaller variations of the pitch angle with radius than those in less luminous grand design spirals; (iii) grand design galaxies show less difference between the pitch angles of individual arms than multi-armed galaxies. Apart from these distinctive features, all three spiral classes do not differ significantly by their pitch angle, arm width, width asymmetry, and environment. Wavelength dependence is found only for the arm fraction. Therefore, observationally we find no strong difference (except for the view and number of arms) between grand design, multi-armed, and flocculent spirals in the sample galaxies.
On the injection of relativistic electrons in the jet of 3C 279
Authors: Hu Wen , Yan Dahai , Dai Benzhong , Zeng Wei , Hu Qianglin
Published: January 2020

The acceleration of electrons in 3C 279 is investigated through analysing the injected electron energy distribution (EED) in a time-dependent synchrotron self-Compton+external Compton emission model. In this model, it is assumed that relativistic electrons are continuously injected into the emission region, and the injected EED [$Q_{\rm e}^\prime (\gamma ^\prime)$] follows a single power-law form with low- and high-energy cut-offs $\rm \gamma _{min}^{\prime }$ and $\rm \gamma _{max}^{\prime }$, respectively, and the spectral index n, i.e. $Q_{\rm e}^\prime (\gamma ^\prime)\propto \gamma ^{\prime -n}$. This model is applied to 14 quasi-simultaneous spectral energy distributions of 3C 279. The Markov Chain Monte Carlo (MCMC)-fitting technique is performed to obtain the best-fitting parameters and the uncertainties on the parameters. The results show that the injected EED is well constrained in each state. The value of n is in the range of 2.5–3.8, which is larger than that expected by the classic non-relativistic shock acceleration. However, the large value of n can be explained by the relativistic oblique shock acceleration. The flaring activity seems to be related to an increased acceleration efficiency, reflected in an increased $\gamma ^{\prime }_{\rm min}$ and electron injection power.
Altered expression of DNA damage repair genes in the brain tissue of mice conceived by in vitro fertilization
Authors: Hu Minhao , Lou Yiyun , Liu Shuyuan , Mao Yuchan , Le Fang , Wang Liya , Li Lejun , Wang Qijing , Li Hongping , Lou Hangying , Wang Ning , Jin Fan
Published: January 2020

Our previous study revealed a higher incidence of gene dynamic mutation in newborns conceived by IVF, highlighting that IVF may be disruptive to the DNA stability of IVF offspring. However, the underlying mechanisms remain unclear. The DNA damage repair system plays an essential role in gene dynamic mutation and neurodegenerative disease. To evaluate the long-term impact of IVF on DNA damage repair genes, we established an IVF mouse model and analyzed gene and protein expression levels of MSH2, MSH3, MSH6, MLH1, PMS2, OGG1, APEX1, XPA and RPA1 and also the amount of H2AX phosphorylation of serine 139 which is highly suggestive of DNA double-strand break (γH2AX expression level) in the brain tissue of IVF conceived mice and their DNA methylation status using quantitative real-time PCR, western blotting and pyrosequencing. Furthermore, we assessed the capacity of two specific non-physiological factors in IVF procedures during preimplantation development. The results demonstrated that the expression and methylation levels of some DNA damage repair genes in the brain tissue of IVF mice were significantly changed at 3 weeks, 10 weeks and 1.5 years of age, when compared with the in vivo control group. In support of mouse model findings, oxygen concentration of in vitro culture environment was shown to have the capacity to modulate gene expression and DNA methylation levels of some DNA damage repair genes. In summary, our study indicated that IVF could bring about long-term alterations of gene and protein expression and DNA methylation levels of some DNA damage repair genes in the brain tissue and these alterations might be resulted from the different oxygen concentration of culture environment, providing valuable perspectives to improve the safety and efficiency of IVF at early embryonic stage and also throughout different life stages.
Generation of pre-tRNAs from polycistronic operons is the essential function of RNase P in Escherichia coli
Authors: Mohanty Bijoy K , Agrawal Ankit , Kushner Sidney R
Published: January 2020

Ribonuclease P (RNase P) is essential for the 5′-end maturation of tRNAs in all kingdoms of life. In Escherichia coli, temperature sensitive mutations in either its protein (rnpA49) and or RNA (rnpB709) subunits lead to inviability at nonpermissive temperatures. Using the rnpA49 temperature sensitive allele, which encodes a partially defective RNase P at the permissive temperature, we show here for the first time that the processing of RNase P-dependent polycistronic tRNA operons to release pre-tRNAs is the essential function of the enzyme, since the majority of 5′-immature tRNAs can be aminoacylated unless their 5′-extensions ≥8 nt. Surprisingly, the failure of 5′-end maturation elicits increased polyadenylation of some pre-tRNAs by poly(A) polymerase I (PAP I), which exacerbates inviability. The absence of PAP I led to improved aminoacylation of 5′-immature tRNAs. Our data suggest a more dynamic role for PAP I in maintaining functional tRNA levels in the cell.
Mental disorders, suicidal ideation, plans and attempts among Canadian police
Authors: Di Nota P M , Anderson G S , Ricciardelli R , Carleton R N , Groll D
Published: January 2020

Background

Recent investigations have demonstrated a significant prevalence of mental health disorders, including post-traumatic stress disorder (PTSD), and suicidal ideation, plans and attempts among Canadian public safety personnel, including police officers. What remains unknown is the relationship between mental disorders and suicide among sworn police officers, and the prevalence of both among civilian police workers.

Aims

To examine the relationship between suicidal ideation, plans and attempts and positive mental health screens for depression, anxiety, panic disorder, alcohol abuse and PTSD among Canadian sworn and civilian police employees.

Methods

Participants completed an online survey that included self-report screening tools for depression, anxiety, panic disorder, alcohol abuse and PTSD. Respondents were also asked if they ever contemplated, planned or attempted suicide. Between-group (Royal Canadian Mounted Police [RCMP], provincial/municipal police and civilians) differences on mental health screening tools were calculated using Kruskal–Wallis analyses. The relationship between mental disorders and suicidal ideation, plans and attempts was evaluated with a series of logistic regressions.

Results

There were 4236 civilian and sworn officer participants in the study. RCMP officers reported more suicidal ideation than other police and scored highest on measures of PTSD, depression, anxiety, stress and panic disorder, which were significantly associated with suicidal ideation and plans but not attempts. Relative to provincial and municipal police, civilians reported more suicide attempts and scored higher on measures of anxiety.

Conclusions

The results identify a strong relationship between mental health disorders and increased risk for suicidal ideation, plans and attempts among sworn and civilian Canadian police employees.
Two Blood Cultures With Age-Appropriate Volume Enhance Suspected Sepsis Decision-Making
Authors: Tran Paul , Dowell Elaine , Hamilton Stacey , Dolan Susan A , Messacar Kevin , Dominguez Samuel R , Todd James
Published: January 2020

Background

Multiple blood cultures have been shown to improve pathogen yield and antimicrobial stewardship for adult patients with suspected serious bacterial infection (SBI). For children, the use of multiple blood cultures is less common and volume recommendations are more complicated, often resulting in single cultures with low volume.

Methods

In 2010, Children’s Hospital Colorado instituted electronic medical record (EMR) decision support to recommend collection of 2 blood cultures before administration of antibiotics for suspected SBI. Recommended blood culture volumes were calculated by age rather than weight. We evaluated all children admitted to inpatient units between 2008 and 2009 (pre-intervention) and 2011 and 2013 (postintervention) who received antibiotics in the hospital after having blood cultures drawn in the emergency department, excluding those with a length of stay >8 days. We compared blood culture yield, isolate classification (pathogen vs contaminant), and antimicrobial modifications before and after the interventions.

Results

A total of 3948 children were included in the study. EMR guidelines were associated with a significantly higher number of children with multiple blood cultures drawn before antibiotic administration (88.0% vs 12.3%; P < .001) and an increased percentage of blood cultures with the recommended volume (74.3% vs 15.2%; P < .001), resulting in a significantly higher pathogen isolation rate and improved antimicrobial decisions. Multiple cultures helped define the role of common contaminants in the clinical decision process.

Conclusions

Multiple blood cultures with age-based volumes taken before starting antibiotics increase pathogen isolation rates and appropriate modification of antimicrobial treatment in children.
FvbHLH9 Functions as a Positive Regulator of Anthocyanin Biosynthesis by Forming a HY5–bHLH9 Transcription Complex in Strawberry Fruits
Authors: Li Yang , Xu Pengbo , Chen Guanqun , Wu Jun , Liu Zhongchi , Lian Hongli
Published: January 2020

Anthocyanin accumulation is transcriptionally regulated by the MYB–bHLH–WD40 complex. Light is indispensable for anthocyanin accumulation, and light-inducible MYB and HY5 were considered to promote anthocyanin accumulation in many fruits. Whether and how light-inducible bHLH transcription factor and HY5 regulate anthocyanin synthesis in strawberry is unknown. In this study, we identified a bHLH transcription factor, FvbHLH9, which was induced by light as well as FvHY5, and found that, similar to FvHY5, the transient overexpression and interference FvbHLH9 in strawberry fruits can promote and decrease anthocyanin accumulation, respectively, indicating FvbHLH9 functions as a positive regulator of anthocyanin biosynthesis. Furthermore, we confirmed that both FvHY5 and FvbHLH9 specifically bind to the promoter region of some key enzyme genes, including FvDFR, and the expression of FvDFR was activated through the heterodimer formation between FvHY5 and FvbHLH9. Finally, we confirmed that FvbHLH9-promoted anthocyanin accumulation is dependent on HY5–bHLH heterodimerisation in Arabidopsis. Our findings provide insights into a mechanism involving the synergistic regulation of light-dependent coloration and anthocyanin biosynthesis via a HY5–bHLH heterodimer formed by the interaction of FvHY5 and FvbHLH9 in strawberry fruits.
Zooplankton growth and survival differentially respond to interactive warming and acidification effects
Authors: Garzke Jessica , Sommer Ulrich , Ismar-Rebitz Stefanie M H
Published: January 2020

The copepod Acartia tonsa is a key component of a wide range of marine ecosystems, linking energy transfer from phytoplankton to higher trophic levels, and has a central role in productivity and biogeochemistry. The interaction of end-of-century global warming and ocean acidification scenarios with testing moderate temperature effects on a seminatural copepod community is needed to understand future community functioning. Here, we deployed a mesocosm experimental set-up with a full factorial design using two temperatures (13°C and 19°C) crossed with a pCO2 gradient ranging from ambient (550 μatm) to 3000 μatm. We used the natural bacteria, phyto- and microzooplankton species composition and biomass of the Kiel Bight and tested the response of A. tonsa development, carbon growth, mortality, size and condition. The tested traits were differently affected by the interaction of temperature and acidification. Ocean acidification increased development, carbon growth, size and mortality under the warming scenario of 19°C. At 13°C mortality rates decreased, while carbon growth, size and condition increased with acidification. We conclude from our experimental approach that a single species shows a variety of responses depending on the focal functional trait. Trait-specific mesozooplankton responses need to be further investigated and compared between geographical regions, seasons and taxonomic groups.
Back to the Future: Using Organization Design Theory for Effective Organizational Networks
Authors: Kenis Patrick , Raab Jörg
Published: January 2020

In this article, we explore how classical organization theory can contribute to better comprehend the phenomenon “organizational networks.” We will demonstrate that this juxtaposition can produce new and interesting insights. First, we argue that an organizational network can be understood as an organization following the classical definition by March and Simon. Second, we assume that organizational networks, as any other form of organizing, need to find solutions to four universal problems as suggested by Puranam and colleagues to be effective: task division, task allocation, reward provision, and information provision. Third, introducing and analyzing an existing organizational network for the management of housing expenses in a Dutch municipality helps us to reflect on how organizational networks in general and this network in particular have found solutions to the four problems of organizing. Finally, we present a number of interesting observations and implications resulting from the confrontation between organizational networks and organization theory and formulate a number of propositions to inspire and guide further research.
Governance mechanisms enabling inter-organizational adaptation: Lessons from grand challenge R&D programs
Authors: Hayter Christopher S , Link Albert N
Published: January 2020

From climate change to terrorism, the world is confronting complex, trans-national problems. As a contemporary response, governments and non-profit organizations have established grand challenge programs, consisting of multi-sector research and development partnerships, to access innovative new ideas and rapidly scale solutions. Following recent scholarly contributions, this article investigates how problems motivating program establishment were identified, how these problems and related contextual factors evolve over time, and how grand challenge programs evolve in response. It does so through a multi-year study of ten grand challenge programs that differ substantially in purpose and organization. This article finds that adaptive capabilities—inter-organizational governance mechanisms—and operational aspects such as purpose, scope, temporal factors, and partner capabilities are critical to program evolution and impact.
Genotypic variability in radial resistance to water flow in olive roots and its response to temperature variations
Authors: López-Bernal Á , García-Tejera O , Testi L , Villalobos F J
Published: January 2020

As radial root resistance (Rp) represents one of the key components of the soil–plant–atmosphere continuum resistance catena modulating water transport, understanding its control is essential for physiologists, modelers and breeders. Reports of Rp, however, are still scarce and scattered in the scientific literature. In this study, we assessed genetic variability in Rp and its dependence on temperature in five widely used olive cultivars. In a first experiment, cultivar differences in Rp at 25 °C were evaluated from flow–pressure measurements in excised roots and subsequent analysis of root traits. In a second experiment, similar determinations were performed continually over a 5-h period in which temperature was gradually increased from 12 to 32 °C, enabling the assessment of Rp response to changing temperature. Despite some variability, our results did not show statistical differences in Rp among cultivars in the first experiment. In the second, cultivar differences in Rp were not significant at 12 °C, but they became so as temperature increased. Furthermore, the changes in Rp between 12 and 32 °C were higher than those expected by the temperature-driven decrease in water viscosity, with the degree of that change differing among cultivars. Also, Rp at 25 °C reached momentarily in the second experiment was consistently higher than in the first at that same, but fixed, temperature. Overall, our results suggest that there is limited variability in Rp among the studied cultivars when plants have been exposed to a given temperature for sufficient time. Temperature-induced variation in Rp might thus be partly explained by changes in membrane permeability that occur slowly, which explains why our values at 25 °C differed between experiments. The observed cultivar differences in Rp with warming also indicate faster acclimation of Rp to temperature changes in some cultivars than others.
Just the once will not hurt: DNA suggests species lumping over two oceans in deep-sea snails (Cryptogemma)
Authors: Zaharias Paul , Kantor Yuri I , Fedosov Alexander E , Criscione Francesco , Hallan Anders , Kano Yasunori , Bardin Jérémie , Puillandre Nicolas
Published: January 2020

The practice of species delimitation using molecular data commonly leads to the revealing of species complexes and an increase in the number of delimited species. In a few instances, however, DNA-based taxonomy has led to lumping together of previously described species. Here, we delimit species in the genus Cryptogemma (Gastropoda: Conoidea: Turridae), a group of deep-sea snails with a wide geographical distribution, primarily by using the mitochondrial COI gene. Three approaches of species delimitation (ABGD, mPTP and GMYC) were applied to define species partitions. All approaches resulted in eight species. According to previous taxonomic studies and shell morphology, 23 available names potentially apply to the eight Cryptogemma species that were recognized herein. Shell morphometrics, radular characters and geographical and bathymetric distributions were used to link type specimens to these delimited species. In all, 23 of these available names are here attributed to seven species, resulting in 16 synonymizations, and one species is described as new: Cryptogemma powelli sp. nov. We discuss the possible reasons underlying the apparent overdescription of species within Cryptogemma, which is shown here to constitute a rare case of DNA-based species lumping in the hyper-diversified superfamily Conoidea.
How molecular allergology can shape the management of allergic airways diseases
Authors: Caimmi Davide , Manca Enrica , Carboni Elena , Demoly Pascal
Published: January 2020

Purpose of review

In allergy, personalized medicine passes through the assessment of molecular allergens sensitization profiles. Such technique may help to better diagnose and treat patients suffering from allergic respiratory diseases.

Recent findings

Different laboratory tests are available today to assess sensitization to molecular allergens, from singleplex assays, to unspecific, screening multiplex assays, mainly performed through microarrays or macroarrays. It is important to collect both results from specific IgE toward allergen extracts and toward molecular allergens, to collect the most complete information on the patient's profile, and therefore to highlight genuine sensitization, and exclude cross-reaction and sensitization because of pan-allergens. Being able to know the exact molecular sensitization profile of the patient, also helps predicting the possible evolution of the disease, and targeting the most appropriate allergen immunotherapy treatment to prescribe.

Even though a cost-effective analysis of running multiple assays in allergic patients has not been performed yet, such technique proved to be more efficient in detecting the appropriate treatment in each patient and in analyzing the true sensitization profile in patients suffering from allergic rhinitis, conjunctivitis, and asthma.