bluuebunny/crossref_metadata_2025 huggingface dataset:
IDENTIFIKASI JENIS TANAMAN SEBAGAI PAKAN LEBAH MADU KELULUT (Trigona spp.) di KPHP KATINGAN HULU
Authors: Alpian Alpian , Yoga Yorlandi Kornelius , Nuwa Nuwa , Yulianti Reri , Joni Herwin , Supriyati Wahyu
Published: December 2022

Identifikasi jenis tumbuhan sebagai pakan lebah kelulut (Trigona spp.) di KPHP Katingan Hulu Unit XVII. Tujuan penelitian adalah untuk mengidentifikasi jenis pakan Lebah Madu Kelulut (Trigona spp.) di KPHP Katingan Hulu Unit XVII dengan metode jalur/metode river survey. Jalur pengamatan ditentukan secara Purposive sampling sebanyak 4 jalur dengan panjang per jalur = 500 m dan jumlah luas jalur secara keseluruhan yaitu 2 Ha. Adapun jumlah seluruh plot pengamatan adalah 21 plot dengan perhitungan luas 21 plot x 400 m2 = 8.400 m2/10.000 m2 = 0,84 Ha dan ukuran plot yaitu 20 m x 20 m dengan jarak antar plot yaitu 100 m. Berdasarkan hasil pengamatan di lapangan didapatkan 25 jenis tanaman yang menjadi pakan lebah kelulut (Trigona spp.). Terdapat 14 jenis vegetasi tingkat semai dengan Indeks Nilai Penting (INP) tertinggi ada pada jenis karamunting (Rhodomyrtus tomentosa) dengan nilai 71,94% dan yang terendah ada pada jenis belimbing waluh (Averrhoa carambola) dengan nilai 4,81%, putri malu (Mimosa pudica) dengan nilai 4,81%, aster putih (Symphyotrichum ericoides) dengan nilai 4,81% dan bayam duri (Amaranthus spinosus) dengan nilai 4,81%. Pada vegetasi tingkat pancang terdapat 4 jenis tanaman  dengan Indeks Nilai Penting (INP) tertinggi ada pada jenis mangga (Mangifera indica) dengan nilai 97,21% dan yang terendah ada pada jenis gaharu (Aquilaria malaccensis) dengan nilai 19,44%. Pada vegetasi tingkat tiang terdapat 6 jenis tanaman dengan Indeks Nilai Penting (INP) tertinggi ada pada jenis akasia (Acacia mangium) dengan nilai 95,81% dan yang terendah ada pada jenis mangga (Mangifera indica) dengan nilai 20,08%. Serta terdapat 10 jenis vegetasi tingkat pohon dengan Indeks Nilai Penting (INP) tertinggi ada pada jenis akasia (Acacia mangium) dengan nilai 110,91% dan yang terendah ada pada jenis johar (Senna siamea) dengan nilai 10,18%.
PENGEMBANGAN LEMBAR KERJA PESERTA DIDIK BERBASIS ETNOMATEMATIKA BUDAYA BANJAR PADA MATERI TEOREMA PYTHAGORAS KELAS VIII SMP/MTS
Authors: Rahmawati Helda , Karim Karim , Hidayanto Taufiq
Published: December 2022

Budaya Banjar merupakan salah satu budaya yang mendominasi di Kalimantan Selatan yang perlu diperkenalkan untuk menumbuhkan sikap menghargai dan menjunjung tinggi arti kebudayaan kepada peserta didik. Pengembangan LKPD berbasis kebudayaan Banjar merupakan salah satu sarana untuk membantu dan mempermudah peserta didik dalam kegiatan pembelajaran sekaligus dapat memperkenalkan serta menumbuhkan sikap menghargai dan menjunjung tinggi arti budaya Banjar itu sendiri. Penelitian pengembangan ini bertujuan untuk menghasilkan LKPD berbasis etnomatematika budaya Banjar pada materi teorema Pythagoras kelas VIII SMP/MTs. Penelitian ini menggunakan metode penelitian pengembangan (research and development) model Plomp. Model Plomp yang digunakan terdiri dari empat fase yaitu diantaranya fase investigasi awal, fase perancangan, fase realisasi/konstruksi, fase tes, evaluasi, dan revisi. Pada fase tes, evaluasi, dan revisi dilakukan uji validitas oleh tiga validator ahli dan uji kepraktisan oleh peserta didik. Hasil uji validitas terhadap perangkat pembelajaran berupa LKPD yang dikembangkan menunjukkan LKPD mencapai kriteria sangat valid dan hasil uji kepraktisan menunjukkan LKPD mencapai kriteria praktis. Sehingga, dihasilkan perangkat pembelajaran berupa LKPD berbasis etnomatematika budaya Banjar materi teorema Pythagoras SMP/MTs kelas VIII yang valid dan praktis.
PENGEMBANGAN E-MODUL BERBASIS HIGHER ORDER THINKING SKILL (HOTS) KONTEKS LINGKUNGAN LAHAN BASAH PADA MATERI ARITMATIKA SOSIAL
Authors: Ningsih Irma , Karim Karim , Budiarti Indah
Published: December 2022

Kurangnya ketersediaan modul yang didesain khusus untuk melatih kemampuan berpikir tingkat tinggi menyebabkan rendahnya kemampuan Higher Order Thinking Skill (HOTS) peserta didik. Oleh karena itu diperlukan modul yang dapat melatih kemampuan berpikir tingkat tinggi. Penelitian ini bertujuan untuk menghasilkan e-modul yang berbasis Higher Order Thinking Skill (HOTS) konteks lingkungan lahan basah pada materi aritmatika sosial. Metode penelitian yang digunakan adalah metode Research and Development (R&D) dengan model pengembangan 4D (Define, Design, Development dan Disseminate). Subjek uji coba penelitian adalah peserta didik kelas VII MTs Siti Mariam Banjarmasin. Teknik pengumpulan data menggunakan instrumen validasi, angket respon peserta didik, dan tes. Validasi dilakukan oleh 3 validator. Uji coba dilakukan kepada 27 peserta didik. Berdasarkan lembar validasi yang diisi validator, dapat disimpulkan e-modul valid karena diperoleh skor rata-rata yaitu 3,31. Berdasarkan angket yang diisi guru dan peserta didik, dapat disimpulkan e-modul praktis karena diperoleh skor kepraktisan dari angket guru yaitu 92,31% dan skor kepraktisan dari angket peserta didik yaitu 82,57%. Berdasarkan uji coba, e-modul dikatakan efektif, karena berdasarkan 3 kali tes formatif, persentase hasil belajar peserta didik lebih dari 80%, yaitu 81,48%, 88,88% dan 88,88%. Berdasarkan hasil yang telah diuraikan, maka dengan demikian kriteria kevalidan dan keefektifan e-modul berbasis Higher Order Thinking Skill (HOTS) konteks lingkungan lahan basah pada materi aritmatika telah terpenuhi.
PENGARUH PENGGUNAAN E-LKPD BERBASIS LIVEWORKSHEETS TERHADAP HASIL BELAJAR DAN KETERAMPILAN BERPIKIR KRITIS PESERTA DIDIK PADA KONSEP PEMBELAHAN SEL
Authors: Annida Siti Fathya , Putra Aminuddin Prahatama , Zaini Muhammad
Published: December 2022

Tujuan dari penelitian ini untuk mendeskripsikan terdapat atau tidaknya pengaruh penggunaan E-LKPD berbasis liveworksheets pada konsep pembelahan sel terhadap hasil belajar kognitif produk, hasil belajar kognitif proses, dan keterampilan berpikir kritis peserta didik. Penelitian ini merupakan penelitian quasi experiment dengan desain pada hasil belajar kognitif menggunakan The Non-equivalent Control Group Design dan keterampilan berpikir kritis menggunakan Posttest-only Control Group Design. Sampel pada penelitian ini diambil dengan teknik purposive sampling. Teknik analisis data menggunakan uji Mann-Whitney. Hasil penelitian menunjukkan bahwa: (1) terdapat pengaruh signifikan pada hasil belajar kognitif produk (sig. 0.005), (2) terdapat pengaruh signifikan pada hasil belajar kognitif proses (sig. 0.007), dan (3) tidak terdapat perbedaan signifikan pada keterampilan berpikir kritis (sig. 0.078). Kesimpulan yang didapatkan pada penelitian ini bahwa penggunaan E-LKPD berbasis liveworksheets dapat meningkatkan hasil belajar peserta didik, namun tidak dapat meningkatkan keterampilan berpikir kritis peserta didik.
KEMAMPUAN LITERASI NUMERASI SISWA SMA KELAS XI MIPA DALAM MATA PELAJARAN FISIKA
Authors: Sartianis Gepi , Yuliati Lia , Parno Parno
Published: December 2022

Penelitian ini bertujuan untuk mengetahui tingkat literasi numerasi siswa kelas XI MIPA. Penelitian ini menggunakan jenis penelitian deskriptif dengan pendekatan kuantitatif. Sampel penelitian ini adalah kelas XI MIPA pada salah satu SMA Negeri di Kabupaten Kerinci Provinsi Jambi dengan jumlah 31 orang dengan teknik pengambilan sampel menggunakan purposive sampling.Soal yang digunakan sebanyak 11 soal tes berbentuk pilihan ganda. Hasil penelitian menunjukkan siswa memiliki kemampuan yang bervariasi. Tingkat kemampuan literasi numerasi siswa berada pada kategori cakap dengan rata-rata 60,9% siswa telah menjawab benar. Kemampuan literasi numerasi siswa berada pada kategori perlu intervensi khusus sebanyak 1 orang, kategori dasar sebanyak 6 orang, kategoricakap sebanyak 18 orang, dan kategorimahir sebanyak 6 orang. Secara keseluruhan siswa memiliki kemampuan yang cukup baik pada indikator mengestimasi dan menghitung; menggunakan pecahan, desimal, persen dan perbandingan; menggunakan penalaran; dan menggunakan pengukuran yang semuanya berada pada kategori cakap dengan persentase jawaban benar berturut-turut 64,52 %, 72,58%, 62,90%, dan 59,68% sedangkan indikator mengnterpretasi informasi masih dalam kategori dasar dengan presentase jawaban benar sebesar 49,46%. Sehingga dapat disimpulkan kemampuan literasi numerasi siswa cukup baik. Namun, masih diperlukan tindakan lebih lanjut dalam mengasah kemampuan siswa terutama dalam meningkatkan literasi numerasi siswa dalam pembelajaran fisika.
PENGEMBANGAN MEDIA POSTER MELALUI APLIKASI CANVA BERMUATAN ETNOSAINS PADA METERI KOLOID UNTUK MENINGKATKAN MOTIVASI DAN HASIL BELAJAR
Authors: Rahmila Rima , Iriani Rilia , Kusasi Muhammad , Leny Leny
Published: December 2022

Telah dilakukan penelitian pengembangan media Poster bermuatan etnosains pada materi koloid bertujuan menghasilkan media belajar yang valid, praktis dan efektif. Model pengembangan yang digunakan adalah model 4D (define, design, development & disseminate). Uji coba media belajar yang dikembangkan dilakukan pada peserta didik SMANegeri 11 Banjarmasin Tahun Ajaran 2021/2022. Uji coba media belajar yang dikembangkan dilakukan pada peserta didik SMA Negeri 11 Banjarmasin kelas XI MIPA 1 yakni uji coba perorangan, kelompok kecil dan terbatas. Teknik pengumpulan data yakni angket (validasi, keterbacaan, respon), observasi dan tes. Analisis data menggunakan analisis deskriptif. Hasil penelitian menunjukkan media yang dikembangkan telah memenuhi kriteria sebagai berikut: (1) sangat valid (aspek kelayakan isi, desain grafis layout, gambar, pengorganisasian dan bahasa) berdasarkan uji kelayakan oleh kelompok ahli; (2) Praktis, berdasarkan uji keterbacaan perorangan dan kelompok, respon peserta didik dan guru; (3) Efektif, berdasarkan hasil uji coba terbatas hasil belajar yang termasuk kriteria tinggi, serta motivasi belajar juga termasuk kriteria tinggi. Secara keseluruhan poster yang dikembangkan mampu meningkatkan motivasi belajar dan hasil belajar peserta didikdan sebagai suatu bentuk media yang mengemukakan etnosains dalam pembelajaran kimia.
PENGARUH MODEL TPS BERBANTUAN VIDEO ANIMASI TERHADAP HASIL BELAJAR KOGNITIF PESERTA DIDIK
Authors: Fanti Sofi Ira , Murtihapsari Murtihapsari , Rombe Yunita Pare
Published: December 2022

Tujuan dari penelitian untuk mengetahui pengaruh model pembelajaran kooperatif tipe TPS (Think Pair Share) berbantuan video animasi terhadap hasil belajar kognitif peserta didik kelas XI IPA di Madrasah Aliyah Negeri Manokwari. Jenis penelitian true eksperimental design dengan desain pretest-posttest control group design. Populasi yang digunakan dari peserta didik kelas XI IPA 2 sebagai kelas eksperimen dan kelas XI IPA 1 sebagai kelas kontrol. Penelitian ini menggunakan teknik purposive sampling dengan jumlah peserta terdiri dari  26 siswa. Hasil analisis uji Paired Sample t-test pada pasangan 1 dan 2 diperoleh nilai sig. (2-tailed) sebesar 0,000 < 0,05, maka H0 ditolak dan Ha diterima sesuai dengan kriteria pengujian hipotesis. Sehinggs dapat disimpulkan bahwa penggunaan model pembelajaran TPS (Think Pair Share) berbantuan video animasi berpengaruh terhadap hasil belajar kognitif peserta didik dengan persen pengaruh yaitu sebesar 60%.
KEMAMPUAN PEMECAHAN MASALAH KOLABORATIF CALON GURU IPA PADA PERKULIAHAN BLENDED LEARNING BERBASIS MULTIKULTURAL
Authors: Wulandari Ria , Shofiyah Noly , Kurniawan Machful Indra
Published: December 2022

Perkembangan teknologi yang massif memungkinkan individu dari berbagai belahan dunia dengan budaya yang berbeda melakukan kolaborasi untuk menyelesaikan masalah dan mencapai tujuan bersama. Sehingga diperlukan suatu keterampilan baru yaitu pemecahan masalah kolaboratif. Tetapi berdasarkan beberapa hasil penelitian diketahui bahwa pemecahan masalah kolaboratif masih rendah, jarang dilatihkan di sekolah dan pelatihan kerja, serta perlu ditingkatkan. Oleh karena itu pada penelitian ini, calon guru IPA akan dilatih pemecahan masalah kolaboratif dalam bentuk perkuliahan blended learning berbasis multikultural.Tujuan penelitian ini adalah mendeskripsikan kemampuan pemecahan masalah kolaboratif berdasarkan komponen-komponen penyusunnya dan respon calon guru IPA terhadap pemecahan masalah kolaboratif dan blended learning. Jenis penelitian adalah penelitian kuantitatif dengan desainone group pretest posttest design. Instrumen yang digunakan adalah tes pemecahan masalah kolaboratif dan lembar angket respon. Hasil penelitian menunjukkan kemampuan pemecahan masalah kolaboratif calon guru IPA mengalami peningkatan yang dapat dilihat dari nilai n-gain sebesar 0,7 (sedang). Calon guru IPA memiliki respon positif terhadap pemecahan masalah kolaboratif sebesar 78,32 dan blended learning sebesar 76,56. Dapat disimpulkan perkuliahan blended learning berbasis multikultural efektif untuk mengajarkan pemecahan masalah kolaboratif dan memberikan respon positif.
PENGARUH PENGGUNAAN FLIPPED CLASSROOM TERHADAP HASIL BELAJAR DAN KEMANDIRIAN PESERTA DIDIK KELAS X PADA PEMBELAJARAN BIOLOGI
Authors: Putra Aminuddin Prahatama , Utami Nurul Hidayati
Published: December 2022

Pembelajaran dengan memanggunakan flipped classroomdibagi atas dua fase berupa (1) fase outclass kelas dengan mengungah bahan sebelum dilaksanakan pembelajaran dengan memanfaatkan LMS berupa Culture Literacy Digital Wetland,  kemudian (2) fase inclass dengan diskusi dan memecahkan masalah. Penelitian ini bertujuan untuk (1) menguji pengaruh flipped classroom pada hasil belajar peserta didik pada materi Biologi dan (2) mendeskripsikan kemandirian belajar peserta didik. Pada penelitian menggunakan penelitian eksperimen semu (quasi experiment) dengan  The non equivalent Kontrol Group pretest-posttest Design. Penelitian dilaksanakan di SMA Negeri 4 Banjarmasin pada kelas  X MIPA 1 sebagai kelas kontrol dan  X MIPA 2 sebagai kelas ekperimen pada tahun ajaran 2021/2022. Instrumen penelitian yang digunakan dalam penelitian tes kognitif terkait dengan hasil belajar dan angket sikap terhadap kemandirian belajar dalam bentuk skala likert.  Hasil penelitian menunjukkan (1) penggunaan flipped clasroom mempengaruh terhadap hasil belajar peserta didik pada materi Biologi dibandingkan kelas kontrol (2) Kemandirian belajar peserta didik menunjukkan pada kategori tinggi pada kelas yang menggunakan flipped clasroom jika dibandingkan pada kelas kontrol yang terkategori sedang
PENGEMBANGAN E-HANDOUT ANDROID BERNUANSA KONTEKSTUAL PADA MATERI PEMANASAN GLOBAL KELAS VII
Authors: Anggraini Feby Ayu , Amri Erismar , Zural Mimin Mardhiah
Published: December 2022

Penilaian ini bertujuan untuk mengembangkan produk e-handout android bernuansa kontekstual pada materi pemanasan global yang digunakan sebagai media pembelajaran sehingga mempermudah guru dalam menjelaskan materi dan dapat memanfaatkan alokasi waktu yang tersedia di sekolah agar tujuan pembelajaran dapat tercapai. Jenis penelitian adalah R&D menggunakan model Plomp dengan subjek uji kelayakan perorangan 3 orang siswa, kelompok kecil 6 orang siswa dan uji kemudahan 32 orang siswa Kelas VII SMP Negeri 10 Padang. Teknik pengumpulan data dilakukan melalui observasi, dan angket. Analisis data menggunakan analisis deskriptif. Hasil penelitian menunjukkan (1) Penilaian ini menghasilkan produk berupa e-handout android yang valid untuk digunakan berdasarkan penilaian dari validator ahli media dengan presentase rata-rata sebesar 97% dengan kategori “Sangat Valid”. (2) Angket kelayakan siswa sebesar 97% dengan kategori “Sangat Layak”. (3) Respon guru dan siswa terhadap angket kemudahan penggunaan e-handout, guru sebesar 98% dan siswa sebesar 93% dengan kategori “Sangat Mudah”. Sehingga pengembangan e-handout android bernuansa kontekstual yang dikembangkan ini telah layak digunakan dalam proses pembelajaran karena telah memenuhi kriteria kevalidan, kelayakan, dan kemudahan dalam penggunaan.
Piperine Reduces Neoplastic Progression in Cervical Cancer Cells by Downregulating the Cyclooxygenase 2 Pathway
Authors: Cardoso Luana Pereira , Sousa Stefanie Oliveira de , Gusson-Zanetoni Juliana Prado , Silva Laura Luciana De Melo Moreira , Frigieri Barbara Maria , Henrique Tiago , Tajara Eloiza Helena , Oliani Sonia Maria , Rodrigues-Lisoni Flávia Cristina
Published: December 2022

Cervical cancer is the fourth most common type of cancer in the world that causes death in women. It is mainly caused by persistent infection by human papillomavirus (HPV) that triggers a chronic inflammatory process. Therefore, the use of anti-inflammatory drugs is a potential treatment option. The effects of piperine, an amino alkaloid derived from Piper nigrum, are poorly understood in cervical cancer inflammation, making it a target of research. This work aimed to investigate the antitumor effect of piperine on cervical cancer and to determine whether this effect is modulated by the cyclooxygenase 2 (PTGS2) pathway using in vitro model of cervical cancer (HeLa, SiHa, CaSki), and non-tumoral (HaCaT) cell lines. The results showed that, piperine reduces in vitro parameters associated with neoplastic evolution such proliferation, viability and migration by cell cycle arrest in the G1/G0 and G2/M phases, with subsequent induction of apoptosis. This action was modulated by downregulated of cyclooxygenase 2 (PTGS2) pathway, which in turn regulates the secretion of cytokines and the expression of mitogen activated protein kinases (MAPKs), metalloproteinases (MMPs), and their antagonists (TIMPs). These findings indicate the phytotherapeutic potential of piperine as complementary treatment in cervical cancer.
Stance4Health nutritional APP: A Path to Personalized Smart Nutrition
Authors: Hinojosa-Nogueira Daniel , Ortiz-Viso Bartolomé , Navajas-Porras Beatriz , Pérez-Burillo Sergio , González-Vigil Verónica , Pastoriza de la Cueva Silvial , Rufián-Henares José Ángel
Published: December 2022

Access to good nutritional health is one of the principal objectives of current society. Several e-services offer dietary advice. However, multifactorial and more individualized nutritional recommendations should be developed to recommend healthy menus according to the specific user's needs. In this article we present and validate a personalized nutrition system based on an application (APP) for smart devices with the capacity to offer an adaptable menu to the user. The APP was developed following a structured recommendation generation scheme, where the characteristics of the menus of 20 users were evaluated. Specific menus were generated for each user based on their preferences and nutritional requirements. These menus were evaluated by comparing their nutritional content versus the nutrient composition retrieved from dietary records. The generated menus showed great similarity to those obtained from the user dietary records. Furthermore, the generated menus showed less variability in micronutrient amounts and higher concentrations than the menus from the user records. The macronutrient deviations were also corrected in the generated menus, offering a better adaptation to the users. The presented system is a good tool for the generation of menus that are adapted to the user characteristics and a starting point to nutritional interventions.
Aquaporin-2 Interaction with Cortisol Modulates its Water Permeability. Perspectives for Corticosteroids Non-genomic Effects
Authors: Mom Robin , Réty Stéphane , Auguin Daniel
Published: December 2022

Aquaporins (AQPs) are water channels widely distributed in living organisms and involved in many pathophysiologies as well as in cell volume regulations (CVR). In the present study, based on the structural homology existing between mineralocorticoids receptors (MR), glucocorticoids receptors (GR), cholesterol consensus motif (CCM) and the extra-cellular vestibules of AQPs, we investigated the binding of corticosteroids on AQP family through in silico molecular dynamics simulations of AQP2 interactions with cortisol. We propose for the first time a putative AQPs corticosteroids binding site (ACBS) and discussed its conservation through structural alignment. Corticosteroids can mediate non-genomic effects, nonetheless, the transduction pathways involved are still misunderstood. Moreover, a growing body of evidence is pointing out toward the existence of a novel membrane receptor mediating part of these rapid corticosteroids effects. Our results suggest that the naturally produced glucocorticoid cortisol inhibits channel water permeability. Based on these results, we propose a detailed description of a putative underlying molecular mechanism. In this process, we also bring new insights on the regulatory function of AQPs extra-cellular loops and on the role of ions in tuning the water permeability. Altogether, this work brings new insights into corticosteroids non-genomic effects through the proposition of AQPs as membrane receptor of this family of regulatory molecules. This original result is the starting point for future investigations to define more in depth and in vivo the validity of this functional model.
Proof of Two-dimensional Jacobian Conjecture
Authors: Su Yucai
Published: December 2022

Using the local bijectivity of Keller maps, we give a proof of two-dimensional Jacobian conjecture.
COMPARATIVE STUDIES OF METHODS FOR IMPROVING THE CYBER SECURITY OF UNMANNED AERIAL VEHICLES WITH THE BUILT-IN ADS-B SYSTEM
Authors: Semenov Serhii , Zhang Min Jian
Published: December 2022

The subject of study in the article is methods for improving the cybersecurity of unmanned aerial vehicles with an integrated ADS-B system. The aim is to analyze and compare methods for improving the cybersecurity of unmanned aerial vehicles with an embedded ADS-B system. Particular scientific tasks: analysis and comparative studies of the main types of cyber threats and methods for improving the cybersecurity of unmanned aerial vehicles with an integrated ADS-B system, the development of an appropriate taxonomy, and the development of proposals for further research. The methods used in the article are the methods of system analysis. The following research results have been obtained: a classification of cyber threats to the security of unmanned aerial vehicles with an integrated ADS-B system has been developed; a taxonomy of ADS-B message modification and injection attacks has been developed; practical UAV security metrics adapted to the presented taxonomy of cyberattacks are defined and researched ; chosen most priority directions research ; - a generalized classification of cryptographic protection methods adapted to solve the problems of improving the security of UAVs with an integrated ADS-B system has been developed; the use of additional means of identification based on steganographic methods of data protection is proposed. Conclusions. An analysis and comparative studies have been carried out, and a generalized classification of methods for improving the cybersecurity of unmanned aerial vehicles with an integrated ADS-B system has been developed. Their advantages and disadvantages are revealed, which made it possible to determine the priority of further research and possible promising ways to solve the tasks.
Feasibility of diffusion kurtosis imaging in evaluating cervical spinal cord injury in multiple sclerosis
Authors: Bian BingYang , Zhou BoXu , Shao ZhiQing , Zhu XiaoNa , Jie YiGe , Li Dan
Published: December 2022

Background and purpose
At present, it is a difficult problem to evaluate cervical spinal cord (CSC) injury in multiple sclerosis (MS). To assess gray matter (GM), white matter (WM), lesions of MS and the thrapeutic effect using diffusion kurtosis imaging (DKI).
Material and Methods
A total of 78 subjects (48 of MS and 30 of health ) perform routine MR scan and DKI of CSC. DKI-metrics were measured in the lesions, normal-appearing GM and WM. The MS patients were divided into two groups according to the presence or absence of T2 hyperintensity. The disability of the two groups was assessed by the extended disability status scale (EDSS) before and after treatment.
Results
1) Significant differences were detected in DKI metrics between MS and healthy (P < 0.05) and between patients with CSC T2-hyperintense and without T2-hyperintense (P < 0.001); 2) Compared to healthy, GM-MK, GM-RK and WM-FA, WM-AD were statistically reduced in patients without T2-hyperintense (P < 0.05). 3) Significant differences were observed in DKI metrics between patients with T2-hyperintense after therapy (P < 0.05), as well as GM-MK and WM-FA, WM-AD in patients without T2-hyperintense (P < 0.05); 4) EDSS was correlated with MK values, as well as EDSS scores and MK values after therapy.
Conclusions
1) DKI-metrics can detect and quantitatively evaluate the changes in cervical spinal cord micropathological structure; 2) MK and RK values are sensitive metrics to detect the damage of gray matter; 3) MK values quantitatively evaluate the clinical disability progression and the therapeutic effect in MS patients.
Self-extracted corn-stalk cellulose/epoxy resin composites
Authors: Lou Chunhua , Jiang siyu , Yan an , Zhou Yongli , Liu Yang , Zhang Yong , Kong Xianzhi
Published: December 2022

In order to make full use of crop waste stalk, corn-stalk cellulose (CSC) was extracted by acid-base method and used as modifier of epoxy resin (E51) to prepare the self-extracted corn-stalk cellulose/epoxy resin composites (CSCEC). Differential scanning calorimeter (DSC), thermogravimetry (TG) analysis, dynamic mechanical analysis (DMA), morphology analysis by scanning electron microscope (SEM), the mechanical properties by electronic universal testing machine and impact testing machine were used for characterization and analysis. The experimental results showed that when the CSC content was 20 wt%, the impact strength of the composite was 2.50 kJ /m2, which was 127.2% higher than that of pure epoxy resin. When the CSC content was 20 wt%, the Tg of epoxy resin obtained by DMA was the lowest, 167.4 ℃, which decreased by 11.3 ℃ compared with that of pure epoxy resin. The SEM result showed that the fracture surface of the composite became obviously rough and had of obvious folds, which was a ductile fracture. These results indicated that the addition of CSC could toughen the epoxy resin.
Genetic testing of sperm donors in China: A survey of current practices
Authors: Huang Chuan , Wang Qi-Lin , Wu Hui-Lan , Luo Xue-Feng , Zhou Wen-Jun , Huang Zeng-Hui , Tang Yu-Ling , Zhang Xin-Zong , Zhu Wen-Bing
Published: December 2022

Background
The National Health and Family Planning Commission of China (NHFPCC) issued the “Measures for the Management of Human Sperm Banks,” which was revised in 2003 and is still in effect today. One of the standard guidelines is that potential donors undergo laboratory testing to exclude infectious and genetic diseases and karyotype analysis. However, patient demands for donor genetic testing have also increased, and only karyotype analysis to exclude genetic diseases is not sufficient to meet these demands.
Materials and Methods
An electronic survey was distributed to twenty-seven sperm banks to examine donor genetic screening practices at sperm banks in China and to evaluate the qualifications and skills of genetic counselors at the banks. Twenty-six human sperm banks responded to a 32-question survey about their current practices related to genetic testing of sperm donors.
Results
The 26 sperm banks reported that all qualified sperm donors undergo karyotype analysis; 22 banks (84.6%) collected three generations of family history from each qualified sperm donor; 10 (38.5%) reported that they attempted to accommodate special requests from donor semen recipients for particular genetic tests. Only 2 of the 26 (7.7%) sperm banks reported that they performed whole-exome sequencing. At all the sperm banks, consent for genetic testing was obtained as part of the overall contract for sperm donors. Nineteen (73.1%) sperm banks had genetic counselors on their staff, while six (23.1%) had no genetic counselors on their staff but had access to genetic counselors at the hospital. Only one (3.8%) sperm bank had no genetic counselors on their staff or at the hospital.
Conclusions
The need for larger scale genetic testing of donors and recipients and an extensive panel of genetic tests specific to the Chinese population. Additionally, professionally trained geneticists must be employed as genetic counsellors so that the results of genetic tests and their implications can be explained to donors.
Evaluating a Research Training Programme for Frontline Health Workers in Conflict-Affected and Fragile Settings in the Middle East
Authors: Naal Hady , Daou Tracy , Brome Dayana , Mansour Rania , Sittah Ghassan Abu , Giannou Christos , Steiger Enrique , Saleh Shadi
Published: December 2022

Background
Health Research Capacity Building (HRCB) is key to improving health research production among health workers in LMICs to inform health policies and reduce health disparities in conflict settings. However, few HRCB programs are available in the MENA region, and few evaluations of HRCB globally are reported in the literature.
Methods
Through a qualitative longitudinal design, we evaluated the first implementation of the Center for Research and Education in the Ecology of War (CREEW) fellowship. Semi-structured interviews were conducted with fellows (n = 5) throughout the program at key phases during their completion of courses and at each research phase. Additional data was collected from supervisors and peers of fellows at their organizations. Data were analysed using qualitative content analysis and presented under pre-identified themes.
Results
Despite the success of most fellows in learning on how to conduct research on AMR in conflict settings and completing the fellowship by producing research outputs, important challenges were identified. Results are categorized under predefined categories of (1) course delivery, (2) proposal development, (3) IRB application, (4) data collection, (5) data analysis, (6) manuscript write-up, (7) long-term effects, and (8) mentorship and networking.
Conclusion
The CREEW model, based on this evaluation, shows potential to be replicable and scalable to other contexts and other health-related topics. Detailed discussion and analysis are presented in the manuscript and synthesized recommendations are highlighted for future programs to consider during the design, implementation, and evaluation of such programs.
Clinical application of double-capsule fecal catheter device in ICU patients with fecal incontinence: a single-center retrospective study across five years
Authors: Chen Shujuan , Liu Yanquan , Zhu Hongquan , Lin Jie , Li Zuotao , Liu Xin , Xie Jiangbo , Xu Qinglin
Published: December 2022

Objective
To explore the clinical effect and potential value of double-capsule fecal catheter device in patients with fecal incontinence in intensive care unit (ICU).
Methods
The double-capsule fecal catheter device in this study has been granted a utility model patent by the State Intellectual Property Office of the People’s Republic of China (Patent grant number: ZL201520691458.6) before its implementation. Design patents have been transformed into physical medical supplies and used in clinical and nursing practice. A total of 62 patients with fecal incontinence who were admitted to the ICU of the First Affiliated Hospital of Gannan Medical University from May 2017 to May 2022 were selected and randomly divided into the experimental group and the control group, with 32 cases in the experimental group and 30 cases in the control group. The experimental group was given a double-capsule fecal catheter device, and the control group was given an ordinary fecal catheter device for drainage. The catheter prolapse rate, catheter obstruction rate, stool leakage rate and prognostic value were compared between the two groups.
Results
The catheter prolapse rate, catheter obstruction rate, and stool leakage rate in the experimental group were significantly lower than those in the control group, and the difference was statistically significant (P < 0.01), and the length of hospital stay and prognostic indicators were better than those in the control group (P < 0.05).
Conclusion
The use of the newly developed double-capsule fecal catheter device can reduce the rate of catheter prolapse, catheter obstruction, and stool leakage in patients with fecal incontinence in the ICU. Correspondingly, patients with fecal incontinence who used the double-capsule fecal catheter device had shorter ICU days than those who did not, and it improved relevant prognostic indicators during the patient's hospitalization. It has good clinical practicability and popularity for fecal incontinence patients, and is worthy of use and promotion.
eTSC-Net: A Parameter-efficient Convolutional Neural Network for Drug Treatment Outcome Studies of Pediatric Epilepsy
Authors: Zhao Cailei , Jiang Dian , Zhao Xia , Yang Jun , Liang Dong , Yuan Bixia , Lin Rongbo , Wang Haifeng , Liao Jianxiang , Hu Zhanqi
Published: December 2022

Background: Ability to predict the outcomes of pharmacological treatment of epilepsy in pediatric patients with tuberous sclerosis complex (TSC) can confer a distinct leverage and guide therapeutic decision-making. Multi-contrast magnetic resonance imaging (MRI) is routinely used for diagnosis of TSC by pediatricians. We propose a parameter-efficient convolutional neural network with multi-contrast images to predict the drug treatment outcomes of pediatric epilepsy in TSC.
Methods: Image-based models were generated using the EfficientNet3D-B0 network architecture. A weighted average ensemble network with multi-contrast images was created as the final model. The proposed neural network is named as Efficient Tuberous sclerosis complex-Net (eTSC-Net).We compared our methods with a Residual Network 3D（ResNet3D) model. We trained a 3D-ResNet on our T2FLAIR data. Binary classification models were trained to distinguish non-controlled group patients from controlled group patients on T2W and T2FLAIR images. We trained all the models using an Nvidia RTX A6000 Graphical Processing Unit (GPU) card. Area under curve(AUC), accuracy (ACC), sensitivity (SEN), and specificity (SPE) were calculated to assess the classification performance for each model in each cohort. The differences between subgroups were assessed using independent samples t test and pvalues < 0.05 were considered indicative of statistical significance.
Results: The proposed neural network (eTSC-Net) achieved the best performance with an AUC value of 0.833 and 90.0% accuracy in the testing cohort, which was better than other models.
Conclusions: The results demonstrated the ability of the proposed method for predicting drug treatment outcomes in pediatric TSC-related epilepsy. eTSC-Net can serve as a useful computer-aided diagnostic tool to help clinical radiologists formulate more targeted treatment.
Association of Molnupiravir and Nirmatrelvir-Ritonavir with Reduced Mortality and All-cause Sepsis in Hospitalized Patients Infected with Omicron Variant of SARS-CoV-2: A Territory-Wide Prospective Cohort Study
Authors: WAI Abraham Ka-chung , LEE Teddy Tai-loy , CHAN Ching-long , CHAN Crystal Ying , YIP Edmond Tsz-fung , LUK Luke Yik-fung , HO Joshua Wing-kei , SO Kevin Wang-leong , TSUI Omar Wai-kiu , LAM Man-lok , LEE Shi-yeow , YAMMAMOTO Tafu , TONG Chak-kwan , WONG Man-sing , WONG Eliza Lai-yi , RAINER Timothy Hudson
Published: December 2022

Object
This study evaluates the association between antivirals (Molnupiravir and Nirmatrelvir-Ritonavir) and all-cause and respiratory mortality and organ dysfunction among high-risk COVID-19 patients during an Omicron outbreak.
Methods
Two cohorts, Nirmatrelvir-Ritonavir vs. control and Molnupiravir vs. control, were constructed with inverse probability treatment weighting to balance baseline characteristics. Cox proportional hazards models evaluated the association of their use with all-cause mortality, respiratory mortality, and all-cause sepsis (a composite of circulatory shock, respiratory failure, acute liver injury, coagulopathy, and acute liver impairment). Patients recruited were hospitalized and diagnosed with the COVID-19 Omicron variant between February 22, 2022 to April 15, 2022, and followed up until May 15, 2022.
Results
The study included 17,704 patients. There were 4.67 and 22.7 total mortalities per 1000 person-days in the Nirmatrelvir-Ritonavir and control groups respectively before adjustment (weighted incidence rate ratio, -18.1 [95%CI, -23.0 to -13.2]; hazard ratio, 0.18 [95%CI, 0.11–0.29]). There were 6.64 and 25.9 total mortalities per 1000 person-days in the Molnupiravir and control groups respectively before adjustment (weighted incidence rate ratio per 1000 person-days, -19.3 [95%CI, -22.6 to -15.9]; hazard ratio, 0.23 [95%CI, 0.18–0.30]). In all-cause sepsis, there were 13.7 and 35.4 organ dysfunction events per 1000 person-days in the Nirmatrelvir-Ritonavir and control groups respectively before adjustment (weighted incidence rate ratio per 1000 person-days, -21.7 [95%CI, -26.3 to -17.1]; hazard ratio, 0.44 [95%CI, 0.38–0.52]). There were 23.7 and 40.8 organ dysfunction events in the Molnupiravir and control groups respectively before adjustment (weighted incidence ratio per 1000 person-days, -17.1 [95%CI, -20.6 to -13.6]; hazard ratio, 0.63 [95%CI, 0.58–0.69]).
Conclusions
Among COVID-19 hospitalized patients, use of either Nirmatrelvir-Ritonavir or Molnupiravir compared with no antiviral use was associated with a significantly lower incidence of 28-day all-cause and respiratory mortality and sepsis.
Minimizing the Makespan and the Carbon Emissions in Green Flexible Job Shop Scheduling Problem with Learning Effect
Authors: Li Zhi , Chen Yingjian
Published: December 2022

One of the most difficult challenges for modern manufacturing is reducing carbon emissions. This paper focuses on the green scheduling problem in a flexible job shop system, taking into account energy consumption and worker learning effects. With the objectives to simultaneously minimze the makespan and total carbon emissions, the green flexible job shop scheduling problem (GFJSP) is formulated as a mixed integer linear multi-objective optimization model. Then the improved multi-objective sparrow search algorithm (IMOSSA) is developed for the optimal solution. Finally, this paper conducted computational experiments, including the the comparison between IMOSSA and the algortithm of GA and Jaya. The resluts demonstrate that the IMOSSA has a high precision, good convergence and excellent performance to solve the GFJSP in low-carbon manufacturing systems.
Simultaneously evaluating efficacy and cardiotoxicity of EGFR-TKI AC0010 with a novel multifunctional biosensor
Authors: Wang Ping , Jiang Deming , Wei Xinwei , Zhu Yuxuan , Qiu Yong , Liu Xin , Kong Liubing , Li Fengheng , Liu Jingwen , Zhuang Liujing , Wan Hao , Ying Kejing
Published: December 2022

Non-small lung cancer (NSCLC) is a leading cause of cancer mortality worldwide. Although epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) have dramatically improved life expectancy for NSCLC patients, concerns about TKI-induced cardiotoxicities increased. AC0010, a novel third-generation TKI, was developed to overcome acquired EGFR-T790M mutation-induced drug resistance. However, the cardiotoxicity of AC0010 remains blurry. To evaluate the efficacy and cardiotoxicity of AC0010, we designed a novel multifunctional biosensor by integrating microelectrodes (MEs) and interdigital electrodes (IDEs) to comprehensively evaluate cells’ viability, electrophysiological activity, and morphologic change (beating for cardiomyocytes). The multifunctional biosensor can monitor AC0010-induced NSCLC inhibition and cardiotoxicity in a quantitatively, label-free, noninvasive, and real-time way. AC0010 was found to significantly inhibit NCI-H1975 (EGFR-L858R/T790M mutation) while weak inhibition was found for A549 (wild-type EGFR). Negligible inhibition was found in the viabilities of HFF-1 (normal fibroblasts) and cardiomyocytes. With the multifunctional biosensor, we found that 10 μM AC0010 significantly affected cardiomyocytes’ extracellular field potential (EFP) and mechanical beating. The amplitude of EFP continuously decreased after AC0010 treatment while the interval decreased first and then increased. We analyzed the change of systole time (ST) and diastole time (DT) within a beating interval and found DT and DT/beating interval rate decreased within one hour after AC0010 treatment, probably indicating insufficient relaxation for cardiomyocytes, which may further aggravate the dysfunction. Here we found that AC0010 significantly inhibit EGFR-mutant NSCLC cells and impair cardiomyocytes’ function at low concentrations (10 μM). It is the first study to evaluate the risk of AC0010-induced cardiotoxicity. In addition, the novel multifunctional biosensors can comprehensively evaluate the anti-tumor efficacy and cardiotoxicity of drugs and candidate compounds.
Expression of IL-13Rα2 and FUS in glioma: clinicopathological and prognostic correlation.
Authors: Cheng Guang , Wang Meng , Zhang Xi-yue , Zhang Yun
Published: December 2022

Background
IL-13Rα2 is one of the most widely studied tumor-associated antigens in glioma research. Fused in sarcoma (FUS) is a DNA/RNA binding protein that is dysfunctional in various malignant tumors. However, the expression of IL-13Rα2 and FUS, their relationship with clinicopathological parameters and their prognostic value in glioma remain unclear.
Methods
In the present study, the expression of IL-13Rα2 and FUS was measured in a glioma tissue array by immunohistochemistry. Pearson’s X2 test was used to determine the correlation between immunohistochemical expressions and clinicopathological parameters. Pearson’s or Spearman correlation test was used to determine the association between these two proteins expression. The Kaplan-Meier analysis was used to investigate the effect of these proteins on prognosis.
Results
The expressions of IL-13Rα2 was significantly higher in high-grade gliomas (HGG) than in low-grade gliomas (LGG) (p=0.000). FUS location was associated with age and Ki-67 expression, whereas IL-13Rα2 expression demonstrated no significant correlation with clinicopathological parameters. Moreover, a positive relationship was found between nuclear and cytoplasmic co-localization FUS and IL-13Rα2 expression (p=0.0054; r=0.3736). Kaplan‒Meier analysis revealed that patients with IL-13Rα2 had worst overall survival (OS) (p=0.0009) compared to other biomarkers. In HGG, IL-13Rα2 combined with nuclear and cytoplasmic co-localization of FUS was associated with even worse OS (p=0.0415).
Conclusion
The co-expression of IL-13Rα2 with nuclear and cytoplasmic FUS may serve as a reliable prognostic biomarker and therapeutic target in glioma.
Quadruplet expanded DNA (QED) genetic code for eukaryotic cells**
Authors: Singh Rama Shankar
Published: December 2022

QED genetic code for eukaryote cells is developed by analyzing triplet gene encoding and overcoming the lack of transcription and splicing regulations. While verifying the triplet genetic code, Nobel laureate H.G. Khorana had trouble synthesizing self-complementarity forming Poly r-dinucleotides adjacent bases, not promoting polypeptide formation, a noncoding. The QED noncoding codon has a similar trait. Here, the QED codon is assumed to comprise all four DNA bases (T, C, A, and G); the code is position-independent and symmetric. The self-complementarity forming adjacent bases (AU) and (C G) with any two NN (N any T, C, A, and G) bases are noncoding. Four DNA bases arranged in a 4x4 square symmetric matrix yield ten independent elements, which set in a 10x10 square symmetric matrix yield 55 independent elements. Under QED assumptions, 55 quadruplets fall into two groups: 20 independent protein-encoding codons and 35 independent noncoding codons applicable to regulating and controlling synthesis, transcription, and splicing processes. Since gene variants lead to dysfunctional protein-causing diseases, steps to correct dysfunctional proteins are described, anticipating a strategy for developing cures for rare diseases and multigenic cancers.

** Patent Pending
A Decennials Study of the Trend of antibiotics studies in China
Authors: YU TONG , Rajasekar Adharsh , Zhang Songhe
Published: December 2022

Antibiotics are one of the greatest inventions in human history and are used worldwide on an enormous scale. Besides its extensive usage in medical and veterinary arenas to treat and prevent the infection, its application is very prominent in other fields, including agriculture, aquaculture, and horticulture. In recent decades, the increased consumption of antibiotics in China saw a vast increase in its production and disposal in various environments. However, in this post-antibiotic era, the abuse and misuse of these valuable compounds could lead to the unreversible consequence of drug resistance. In China, antibiotics are given a broad discussion in various fields to reveal their impact on both human/animals health and the environment. To our knowledge, we are the first paper to look back at the development trend of antibiotic-related studies in China with qualitative and quantitative bibliometric analysis from the past decades. Our study identified and analysed 5559 papers from its inception (1991) to December 6, 2021, from the Web of Science Core Collection database. However, with few authors and institutions focusing on long term studies, we found the quality of contributions were uneven. Studies mainly focused on areas such as food science, clinical research, and environmental studies, including Molecular biology, genetics and environmental, ecotoxicology, and nutrition, which indicate possible primary future trends. Our study reports on including potentially new keywords, studies' milestones, and their contribution to antibiotic research. We offer potential topics that may be important in upcoming years that could help guide future research.
Synthesis and α-glucosidase inhibitory activity of cinnnamamides
Authors: Aijijiyah Nur Pasca , Wati First Ambar , Rahayu Reni , Srilisti Alfatchu , Mahzumi Farah , Aulia Tinezsia , Santoso Liangga , Pamela Egar , Ramadhani Ersya Yanu , Ilfahmi Yan Alamanda , Purnomo Adi Setyo , Putra Surya Rosa , Santoso Eko , Ningsih Sri , Firdausi Nisrina , Santoso Mardi
Published: December 2022

Suitably substituted cinnnamamides were successfully synthesized in 11-92% yield. Majority of the cinnnamamides displayed IC50 greater than acarbose. Their α-glucosidase inhibitory activity greatly depended on their structure with electron-withdrawing groups on the cinnamoyl aromatic ring causing increased inhibition activity. The synthesized cinnnamamides showed acceptable physicochemical and pharmacokinetics characteristics with little toxicity indicating their potential use as lead drug candidates. Molecular docking studies of these compounds were also conducted to elucidate their mechanism of action. Overall, these cinnnamamides show potential as lead structures for further optimization as α-glucosidase inhibitors.
Progesterone activation of β1-containing BK channels involves two novel binding sites
Authors: North Kelsey , Shaw Andrew , Bukiya Anna , DOPICO ALEX
Published: December 2022

Progesterone at ≥1 µM is used in recovery of cerebral ischemia, an effect likely contributed to by cerebrovascular dilation. The targets of this progesterone action are unknown. Activation of cerebrovascular myocyte BK channels, which include channel-forming α and regulatory β1 subunits, causes cerebrovascular dilation. We report that µM progesterone activates mouse cerebrovascular myocyte BK channels; this action is lost in β1-/- mice and α subunit homotetramers reconstituted into lipid bilayers but sustained in β1/β4-containing heteromers. Progesterone binds to both regulatory subunits, and two novel steroid binding sites, conserved in β1-β4 are unveiled: high affinity (sub-µM), which involves Trp87 in β1 loop, and low affinity (µM) defined by TM1 Tyr32 and TM2 Trp163. Probing mutant channels with progesterone and its oxime-analog indicates that progesterone-loop binding facilitates binding to the low-affinity site where a single progesterone molecule bridges Tyr32-Trp163 to activate BK channels, thus unveiling a novel model of steroid-ion channel interactions.