bluuebunny/crossref_metadata_2025 huggingface dataset:
PINK1/Parkin-mediated mitophagy inhibits osteoblast apoptosis induced by advanced oxidation protein products
Authors: Li Wei , Jiang Wang-Sheng , Su Ya-Ru , Tu Ke-Wu , Zou Lin , Liao Cong-Rui , Wu Qian , Wang Zi-Han , Zhong Zhao-Ming , Chen Jian-Ting , Zhu Si-Yuan
Published: February 2023

Osteoblast apoptosis plays an important role in age-related bone loss and osteoporosis. Our previous study revealed that advanced oxidation protein products (AOPPs) could induce nicotinamide adenine dinucleotide phosphate oxidase (NOX)-derived reactive oxygen species (ROS) production, cause mitochondrial membrane potential (ΔΨm) depolarization, trigger the mitochondria-dependent intrinsic apoptosis pathway, and lead to osteoblast apoptosis and ultimately osteopenia and bone microstructural destruction. In this study, we found that AOPPs also induced mitochondrial ROS (mtROS) generation in osteoblastic MC3T3-E1 cells, which was closely related to NOX-derived ROS, and aggravated the oxidative stress condition, thereby further promoting apoptosis. Removing excessive ROS and damaged mitochondria is the key factor in reversing AOPP-induced apoptosis. Here, by in vitro studies, we showed that rapamycin further activated PINK1/Parkin-mediated mitophagy in AOPP-stimulated MC3T3-E1 cells and significantly alleviated AOPP-induced cell apoptosis by eliminating ROS and damaged mitochondria. Our in vivo studies revealed that PINK1/Parkin-mediated mitophagy could decrease the plasma AOPP concentration and inhibit AOPP-induced osteoblast apoptosis, thus ameliorating AOPP accumulation-related bone loss, bone microstructural destruction and bone mineral density (BMD) loss. Together, our study indicated that therapeutic strategies aimed at upregulating osteoblast mitophagy and preserving mitochondrial function might have potential for treating age-related osteoporosis.
RBM3 suppresses stemness remodeling of prostate cancer in bone microenvironment by modulating N6-methyladenosine on CTNNB1 mRNA
Authors: Zhang Shouyi , Lv Chengcheng , Niu Yichen , Li Changqi , Li Xiuming , Shang Yu , Zhang Yunchao , Zhang Yue , Zhang Yong , Zeng Yu
Published: February 2023

Bone metastasis is the most happened metastatic event in prostate cancer (PCa) and needs a large effort in treatment. When PCa metastasizes to the bone, the new microenvironment can induce the epigenome reprogramming and stemness remodeling of cancer cells, thereby increasing the adaptability of cancer cells to the bone microenvironment, and this even leads to the occurrence of secondary tumor metastasis. Our group has previously found that RNA binding motif 3 (RBM3) affects the stem cell-like properties of PCa by interfering with alternative splicing of CD44. However, whether RBM3, as a stress-response protein, can resist microenvironmental remodeling of PCa particularly in bone metastasis remains unknown. By co-culturing PCa cells with osteoblasts to mimic PCa bone metastases, we found that RBM3 upregulates the N6-methyladenosine (m6A) methylation on the mRNA of catenin beta 1 (CTNNB1) in a manner dependent on methyltransferase 3 (METTL3), an N6-adenosine-methyltransferase complex catalytic subunit. Consequently, this modification results in a decreased stability of CTNNB1 mRNA and a followed inactivation of Wnt signaling, which ultimately inhibits the stemness remodeling of PCa cells by osteoblasts. Thus, the present study may extend our understanding of the inhibitory role of RBM3 on particularly bone metastasis of PCa.
Polo-like kinase 4 inhibitor CFI-400945 inhibits carotid arterial neointima formation but increases atherosclerosis
Authors: Sun Jiaxing , Belke Darrell , Gui Yu , Chen Yong-Xiang , Zhou Shenghua , Zheng Xi-Long
Published: February 2023

Neointima lesion and atherosclerosis are proliferative vascular diseases associated with deregulated proliferation of vascular smooth muscle cells (SMCs). CFI-400945 is a novel, highly effective anticancer drug that inhibits polo-like kinase 4 (PLK4) and targets mitosis. In this study, we aim to investigate how CFI-400945 affects the development of proliferative vascular diseases. In C57BL/6 mice, neointima formation was generated by complete carotid ligation. In apolipoprotein E knockout (ApoE−/−) mice fed a high-fat diet, atherosclerosis was induced by partial carotid ligation. CFI-400945 was directly applied to carotid arteries via a perivascular collar. Our results showed that CFI-400945 drastically inhibited neointima formation but significantly accelerated atherosclerosis. In vitro studies showed that CFI-400945 treatment induced SMC polyploidization and arrested cells in the G2/M phase. CFI-400945 treatment upregulated p53 and p27 expression but decreased p21 and cyclin B1 expression. CFI-400945 also induced SMC apoptosis, which was inhibited by hydroxyurea, a DNA synthesis inhibitor that inhibits polyploidization. Furthermore, CFI-400945 caused supernumerary centrosomes, leading to mitotic failure, resulting in polyploidization. In conclusion, CFI-400945 prevents carotid arterial neointima formation in C57BL/6 mice but accelerates atherosclerosis in ApoE−/− mice, likely through mitotic arrest and subsequent induction of polyploidization and apoptosis.
Bulk evidence of anisotropic s-wave pairing with no sign change in the kagome superconductor CsV3Sb5
Authors: Roppongi M. , Ishihara K. , Tanaka Y. , Ogawa K. , Okada K. , Liu S. , Mukasa K. , Mizukami Y. , Uwatoko Y. , Grasset R. , Konczykowski M. , Ortiz B. R. , Wilson S. D. , Hashimoto K. , Shibauchi T.
Published: February 2023

The recently discovered kagome superconductorsAV3Sb5(A= K, Rb, Cs) exhibit unusual charge-density-wave (CDW) orders with time-reversal and rotational symmetry breaking. One of the most crucial unresolved issues is identifying the symmetry of the superconductivity that develops inside the CDW phase. Theory predicts a variety of unconventional superconducting symmetries with sign-changing and chiral order parameters. Experimentally, however, superconducting phase information inAV3Sb5is still lacking. Here we report the impurity effects in CsV3Sb5using electron irradiation as a phase-sensitive probe of superconductivity. Our magnetic penetration depth measurements reveal that with increasing impurities, an anisotropic fully-gapped state changes to an isotropic full-gap state without passing through a nodal state. Furthermore, transport measurements under pressure show that the double superconducting dome in the pressure-temperature phase diagram survives against sufficient impurities. These results support that CsV3Sb5is a non-chiral, anisotropics-wave superconductor with no sign change both at ambient and under pressure.
Dispersed surface Ru ensembles on MgO(111) for catalytic ammonia decomposition
Authors: Fang Huihuang , Wu Simson , Ayvali Tugce , Zheng Jianwei , Fellowes Joshua , Ho Ping-Luen , Leung Kwan Chee , Large Alexander , Held Georg , Kato Ryuichi , Suenaga Kazu , Reyes Yves Ira A. , Thang Ho Viet , Chen Hsin-Yi Tiffany , Tsang Shik Chi Edman
Published: February 2023

Ammonia is regarded as an energy vector for hydrogen storage, transport and utilization, which links to usage of renewable energies. However, efficient catalysts for ammonia decomposition and their underlying mechanism yet remain obscure. Here we report that atomically-dispersed Ru atoms on MgO support on its polar (111) facets {denoted as MgO(111)} show the highest rate of ammonia decomposition, as far as we are aware, than all catalysts reported in literature due to the strong metal-support interaction and efficient surface coupling reaction. We have carefully investigated the loading effect of Ru from atomic form to cluster/nanoparticle on MgO(111). Progressive increase of surface Ru concentration, correlated with increase in specific activity per metal site, clearly indicates synergistic metal sites in close proximity, akin to those bimetallic N2 complexes in solution are required for the stepwise dehydrogenation of ammonia to N2/H2, as also supported by DFT modelling. Whereas, beyond surface doping, the specific activity drops substantially upon the formation of Ru cluster/nanoparticle, which challenges the classical view of allegorically higher activity of coordinated Ru atoms in cluster form (B5 sites) than isolated sites.
Suppression of kernel vibrations by layer-by-layer ligand engineering boosts photoluminescence efficiency of gold nanoclusters
Authors: Zhong Yuan , Zhang Jiangwei , Li Tingting , Xu Wenwu , Yao Qiaofeng , Lu Min , Bai Xue , Wu Zhennan , Xie Jianping , Zhang Yu
Published: February 2023

The restriction of structural vibration has assumed great importance in attaining bright emission of luminescent metal nanoclusters (NCs), where tremendous efforts are devoted to manipulating the surface landscape yet remain challenges for modulation of the structural vibration of the metal kernel. Here, we report efficient suppression of kernel vibration achieving enhancement in emission intensity, by rigidifying the surface of metal NCs and propagating as-developed strains into the metal core. Specifically, a layer-by-layer triple-ligands surface engineering is deployed to allow the solution-phase Au NCs with strong metal core-dictated fluorescence, up to the high absolute quantum yields of 90.3 ± 3.5%. The as-rigidified surface imposed by synergistic supramolecular interactions greatly influences the low-frequency acoustic vibration of the metal kernel, resulting in a subtle change in vibration frequency but a reduction in amplitude of oscillation. This scenario therewith impedes the non-radiative relaxation of electron dynamics, rendering the Au NCs with strong emission. The presented study exemplifies the linkage between surface chemistry and core-state emission of metal NCs, and proposes a strategy for brighter emitting metal NCs by regulating their interior metal core-involved motion.
JNK signaling in pioneer neurons organizes ventral nerve cord architecture in Drosophila embryos
Authors: Karkali Katerina , Saunders Timothy E. , Panayotou George , Martín-Blanco Enrique
Published: February 2023

Morphogenesis of the Central Nervous System (CNS) is a complex process that obeys precise architectural rules. Yet, the mechanisms dictating these rules remain unknown. Analyzing morphogenesis of theDrosophilaembryo Ventral Nerve Cord (VNC), we observe that a tight control of JNK signaling is essential for attaining the final VNC architecture. JNK signaling in a specific subset of pioneer neurons autonomously regulates the expression of Fasciclin 2 (Fas 2) and Neurexin IV (Nrx IV) adhesion molecules, probably via the transcription factorzfh1. Interfering at any step in this cascade affects fasciculation along pioneer axons, leading to secondary cumulative scaffolding defects during the structural organization of the axonal network. The global disorder of architectural landmarks ultimately influences nervous system condensation. In summary, our data point to JNK signaling in a subset of pioneer neurons as a key element underpinning VNC architecture, revealing critical milestones on the mechanism of control of its structural organization.
Antitumour activity of neratinib in patients with HER2-mutant advanced biliary tract cancers
Authors: Harding James J. , Piha-Paul Sarina A. , Shah Ronak H. , Murphy Jessica J. , Cleary James M. , Shapiro Geoffrey I. , Quinn David I. , Braña Irene , Moreno Victor , Borad Mitesh , Loi Sherene , Spanggaard Iben , Park Haeseong , Ford James M. , Arnedos Mónica , Stemmer Salomon M. , de la Fouchardiere Christelle , Fountzilas Christos , Zhang Jie , DiPrimeo Daniel , Savin Casey , Duygu Selcuklu S. , Berger Michael F. , Eli Lisa D. , Meric-Bernstam Funda , Jhaveri Komal , Solit David B. , Abou-Alfa Ghassan K.
Published: February 2023

HER2 mutations are infrequent genomic events in biliary tract cancers (BTCs). Neratinib, an irreversible, pan-HER, oral tyrosine kinase inhibitor, interferes with constitutive receptor kinase activation and has activity in HER2-mutant tumours. SUMMIT is an open-label, single-arm, multi-cohort, phase 2, ‘basket’ trial of neratinib in patients with solid tumours harbouring oncogenic HER2 somatic mutations (ClinicalTrials.gov: NCT01953926). The primary objective of the BTC cohort, which is now complete, is first objective response rate (ORR) to neratinib 240 mg orally daily. Secondary objectives include confirmed ORR, clinical benefit rate, progression-free survival, duration of response, overall survival, safety and tolerability. Genomic analyses were exploratory. Among 25 treatment-refractory patients (11 cholangiocarcinoma, 10 gallbladder, 4 ampullary cancers), the ORR is 16% (95% CI 4.5–36.1%). The most common HER2 mutations are S310F (n = 11; 48%) and V777L (n = 4; 17%). Outcomes appear worse for ampullary tumours or those with co-occurring oncogenic TP53 and CDKN2A alterations. Loss of amplified HER2 S310F and acquisition of multiple previously undetected oncogenic co-mutations are identified at progression in one responder. Diarrhoea is the most common adverse event, with any-grade diarrhoea in 14 patients (56%). Although neratinib demonstrates antitumour activity in patients with refractory BTC harbouring HER2 mutations, the primary endpoint was not met and combinations may be explored.
The feasibility and impact of implementing a computer-guided consultation to target health inequality in Asthma
Authors: Chakrabarti B. , Kane B. , Barrow C. , Stonebanks J. , Reed L. , Pearson M. G. , Davies L. , Osborne M. , England P. , Litchfield D. , McKnight E. , Angus R. M.
Published: February 2023

Greater Manchester has a greater prevalence and worse asthma outcomes than the national average. This study aims to evaluate a digital approach to primary care asthma management and in particular the initial impact of implementing Clinical Decision Support System software in the form of a computer-guided consultation (CGC) in the setting of primary care asthma reviews in deprived areas of Greater Manchester. The CGC (LungHealth Ltd) is an intelligent decision support system ensuring accurate guideline-based staging of asthma and assessment of asthma control with the software subsequently prompting guideline-standard management. Patients on asthma registers in Greater Manchester Primary Care Networks were identified and underwent remote review by nursing staff using the CGC linked directly to the GP clinical system. Three-hundred thirty-eight patients (mean age 59 (SD 17) years; 60% Female) were reviewed. The CGC reported the patient’s asthma control to be “Good” in 22%, “Partial” in 6% and “Poor” in 72%. ACT scores were significantly higher in those patients exhibiting “Good” and “Partial” control when compared to those with “Poor” control. The number of steroid courses and hospital admissions in the previous 12 months was significantly lower in those patients exhibiting “Good” and “Partial” control when compared to those with “Poor” control. Nineteen percent were found not to have a personalised asthma management plan during CGC review, which was alerted by the CGC and subsequently, all but 3 patients had this created on review completion (McNemar’s test; p < 0.001). 5% were found not to have been prescribed regular inhaled steroid therapy resulting in the operator being alerted by the CGC in all cases. Overall, 44% underwent alteration in asthma therapy following the CGC review with 82% of these representing treatment escalation. An end-to-end digital service solution is feasible for Asthma within primary care and the utilisation of a CGC when conducting primary care asthma reviews increases implementation of guideline-level management thus addressing healthcare inequality while enabling identification of “high risk” asthma patients and guiding appropriate therapy escalation and de-escalation.
Exploring individual differences in musical rhythm and grammar skills in school-aged children with typically developing language
Authors: Nitin Rachana , Gustavson Daniel E. , Aaron Allison S. , Boorom Olivia A. , Bush Catherine T. , Wiens Natalie , Vaughan Chloe , Persici Valentina , Blain Scott D. , Soman Uma , Hambrick David Z. , Camarata Stephen M. , McAuley J. Devin , Gordon Reyna L.
Published: February 2023

A growing number of studies have shown a connection between rhythmic processing and language skill. It has been proposed that domain-general rhythm abilities might help children to tap into the rhythm of speech (prosody), cueing them to prosodic markers of grammatical (syntactic) information during language acquisition, thus underlying the observed correlations between rhythm and language. Working memory processes common to task demands for musical rhythm discrimination and spoken language paradigms are another possible source of individual variance observed in musical rhythm and language abilities. To investigate the nature of the relationship between musical rhythm and expressive grammar skills, we adopted an individual differences approach in N = 132 elementary school-aged children ages 5–7, with typical language development, and investigated prosodic perception and working memory skills as possible mediators. Aligning with the literature, musical rhythm was correlated with expressive grammar performance (r = 0.41, p < 0.001). Moreover, musical rhythm predicted mastery of complex syntax items (r = 0.26, p = 0.003), suggesting a privileged role of hierarchical processing shared between musical rhythm processing and children’s acquisition of complex syntactic structures. These relationships between rhythm and grammatical skills were not mediated by prosodic perception, working memory, or non-verbal IQ; instead, we uncovered a robust direct effect of musical rhythm perception on grammatical task performance. Future work should focus on possible biological endophenotypes and genetic influences underlying this relationship.
Transportation of nanomaterial Maxwell fluid flow with thermal slip under the effect of Soret–Dufour and second-order slips: nonlinear stretching
Authors: Abbas Nadeem , Shatanawi Wasfi , Shatnawi Taqi A. M.
Published: February 2023

In this study, impact of second order slip for Maxwell fluid at vertical exponential stretching sheet is deliberated. Dufour and Soret impact for vertical exponential stretching sheet under nonlinear radiation are deliberated. Thermal and concentration slips with viscous dissipation are taken into account under the Buongiorno’s model. Under the above assumptions, the differential model constructed using the boundary layer approximations using the governing equations. The similarities transformations are introduced which applied the differential model (partial differential equations) and developed the dimensionless differential equations (ordinary differential equations). The dimensionless differential equations are cracked by numerical scheme. The impact of physical parameters are presented by tables and graphs. The curves of fluid velocity enhanced due to increasing the values of velocity slip. Velocity slip is a fluid-boundary interaction in physics. If the velocity slip increased, the fluid velocity profile would eventually become increasing. Temperature curves declined by improving values of $${K}_{1}$$

K
1

. The thermal thickness reduced when improved the values of $${K}_{1}$$

K
1

.
Using machine learning to predict venous thromboembolism and major bleeding events following total joint arthroplasty
Authors: Shohat Noam , Ludwick Leanne , Sherman Matthew B. , Fillingham Yale , Parvizi Javad
Published: February 2023

Venous thromboembolism (VTE) and major bleeding (MBE) are feared complications that are influenced by numerous host and surgical related factors. Using machine learning on contemporary data, our aim was to develop and validate a practical, easy-to-use algorithm to predict risk for VTE and MBE following total joint arthroplasty (TJA). This was a single institutional study of 35,963 primary and revision total hip (THA) and knee arthroplasty (TKA) patients operated between 2009 and 2020. Fifty-six variables related to demographics, comorbidities, operative factors as well as chemoprophylaxis were included in the analysis. The cohort was divided to training (70%) and test (30%) sets. Four machine learning models were developed for each of the outcomes assessed (VTE and MBE). Models were created for all VTE grouped together as well as for pulmonary emboli (PE) and deep vein thrombosis (DVT) individually to examine the need for distinct algorithms. For each outcome, the model that best performed using repeated cross validation was chosen for algorithm development, and predicted versus observed incidences were evaluated. Of the 35,963 patients included, 308 (0.86%) developed VTE (170 PE’s, 176 DVT’s) and 293 (0.81%) developed MBE. Separate models were created for PE and DVT as they were found to outperform the prediction of VTE. Gradient boosting trees had the highest performance for both PE (AUC-ROC 0.774 [SD 0.055]) and DVT (AUC-ROC 0.759 [SD 0.039]). For MBE, least absolute shrinkage and selection operator (Lasso) analysis had the highest AUC (AUC-ROC 0.803 [SD 0.035]). An algorithm that provides the probability for PE, DVT and MBE for each specific patient was created. All 3 algorithms had good discriminatory capability and cross-validation showed similar probabilities comparing predicted and observed failures indicating high accuracy of the model. We successfully developed and validated an easy-to-use algorithm that accurately predicts VTE and MBE following TJA. This tool can be used in every-day clinical decision making and patient counseling.
Identifying congenital generalized lipodystrophy using deep learning-DEEPLIPO
Authors: da Cunha Olegario Natália Bitar , da Cunha Neto Joel Sotero , Barbosa Paulo Cirillo Souza , Pinheiro Plácido Rogério , Landim Pedro Lino Azevêdo , Montenegro Ana Paula Dias Rangel , Fernandes Virginia Oliveira , de Albuquerque Victor Hugo Costa , Duarte João Batista Furlan , da Cruz Paiva Lima Grayce Ellen , Junior Renan Magalhães Montenegro
Published: February 2023

Congenital Generalized Lipodystrophy (CGL) is a rare autosomal recessive disease characterized by near complete absence of functional adipose tissue from birth. CGL diagnosis can be based on clinical data including acromegaloid features, acanthosis nigricans, reduction of total body fat, muscular hypertrophy, and protrusion of the umbilical scar. The identification and knowledge of CGL by the health care professionals is crucial once it is associated with severe and precocious cardiometabolic complications and poor outcome. Image processing by deep learning algorithms have been implemented in medicine and the application into routine clinical practice is feasible. Therefore, the aim of this study was to identify congenital generalized lipodystrophy phenotype using deep learning. A deep learning approach model using convolutional neural network was presented as a detailed experiment with evaluation steps undertaken to test the effectiveness. These experiments were based on CGL patient’s photography database. The dataset consists of two main categories (training and testing) and three subcategories containing photos of patients with CGL, individuals with malnutrition and eutrophic individuals with athletic build. A total of 337 images of individuals of different ages, children and adults were carefully chosen from internet open access database and photographic records of stored images of medical records of a reference center for inherited lipodystrophies. For validation, the dataset was partitioned into four parts, keeping the same proportion of the three subcategories in each part. The fourfold cross-validation technique was applied, using 75% (3 parts) of the data as training and 25% (1 part) as a test. Following the technique, four tests were performed, changing the parts that were used as training and testing until each part was used exactly once as validation data. As a result, a mean accuracy, sensitivity, and specificity were obtained with values of [90.85 ± 2.20%], [90.63 ± 3.53%] and [91.41 ± 1.10%], respectively. In conclusion, this study presented for the first time a deep learning model able to identify congenital generalized lipodystrophy phenotype with excellent accuracy, sensitivity and specificity, possibly being a strategic tool for detecting this disease.
The importance of the Andes in the evolutionary radiation of Sigmodontinae (Rodentia, Cricetidae), the most diverse group of mammals in the Neotropics
Authors: Vallejos-Garrido Paulo , Pino Kateryn , Espinoza-Aravena Nicolás , Pari Alexander , Inostroza-Michael Oscar , Toledo-Muñoz Macarena , Castillo-Ravanal Boris , Romero-Alarcón Viviana , Hernández Cristián E. , Palma R. Eduardo , Rodríguez-Serrano Enrique
Published: February 2023

The Andean mountains stand out for their striking species richness and endemicity that characterize many emblematic Neotropical clades distributed in or around these mountains. The radiation of the Sigmodontinae subfamily, the most diversified mammalian group in the Neotropics, has been historically related to Andean orogenesis. We aim to evaluate this interplay between geological processes and biological responses through the diversification dynamics, the biogeographical history, and the range evolution of the subfamily. For these, we built the most comprehensive phylogeny and gathered 14,836 occurrences for the subfamily. We identified one shift in the speciation rate in the genusAkodon, which suffered their Andean radiation after the arrival of non-Andean ancestors. Our biogeographic analyses show multiple dispersal paths throughout the evolution that allowed this subfamily to colonize all Neotropics. The Northern Andes and Central-Southern Andes were the most important sources of diversity. In addition, the Central-Southern Andes were the most relevant sink, receiving the highest number of lineages. The Andean region exhibited higher speciation and turnover rates than non-Andean regions. Thus, our results support the crucial role of the Andean Mountains in the Sigmodontinae radiation, acting as a "macroevolutionary cradle" and "species attractor" for several sigmodontine lineages at different times, and as a "species pump" becoming the biogeographic source of multiple widely distributed neotropical lineages. Then, complex macroevolutionary dynamics would explain these rodents' high extant Andean diversity and their wide distribution in the Neotropics.
Restoration of insect communities after land use change is shaped by plant diversity: a case study on carabid beetles (Carabidae)
Authors: Lange Markus , Ebeling Anne , Voigt Winfried , Weisser Wolfgang
Published: February 2023

There is no doubt about the insect decline currently taking place in ecosystems with large anthropogenic impacts. Thus, there is a need for practices that avoid insect decline and or help to recover insect communities that have already suffered. Plant diversity has been shown to be positively related to insect abundance and diversity and to ecosystem functions provided by insects. However, it remains open if increased plant diversity can help to recover decreased populations. Here, we tested over one decade the effects of plant diversity on the carabid community in a large grassland biodiversity experiment and how plant diversity fostered the establishment of a natural grassland community after conversion of an arable field. There was a dramatic decline in carabid abundance from 2003, the first year after establishing the diversity experiment, to 2005. However, subsequently, the abundance increased constantly. One year after the land use change most individuals and species were those commonly found in agricultural fields. In subsequent years the community was dominated by grassland species. While plant diversity did not affect the abundance and richness of the carabid community, the turnover to a more native grassland community was accelerated by plant diversity in the first years after the land use change. In contrast, in later years plant diversity stabilized the community assemblage. Our study shows that high plant diversity can contribute to a faster transition of insect populations towards naturally occurring community assemblages and at later stages to more stabilized assemblages.
Evaluation and remediation protocol of selected organochlorine pesticides and heavy metals in industrial wastewater using nanoparticles (NPs) in Nigeria
Authors: Nnaji Jude Chidozie , Amaku James Friday , Amadi Okoche Kelvin , Nwadinobi Solomon Ireji
Published: February 2023

Limited knowledge of the level of contaminants in industrial wastewater within the Nigerian states together with the global challenge of water supply have compelled our investigation into the analyses and removal of organochlorine pesticides (OCPs) and heavy metal contents in industrial wastewater. Wastewater samples were collected from 13 industries across five states in Nigeria. The OCPs content of the samples was extracted, cleaned up and analysed using gas chromatography-mass spectrometry. The results indicate that the mean concentrations of the OCPs in the effluent samples ranged from 1.76 ng L−1 (Dieldrin) to 0.89 ng L−1 (endrin). Cadmium (Cd), chromium (Cr) and lead (Pb) were evaluated in all the effluent water samples. The results show that the average concentrations of the heavy metal ions in the effluent samples ranged from 0.008 ± 0.003 mg L−1 (Cd) to 2.215 ± 0.841 mg L−1 (Pb). For the removal of the identified contaminants, biomagnetite nanoparticles (BioMag), magnetite nanoparticles (MagNPs), biomagnetite-CMC nanocomposite (BioMag-CMC) and magnetite-CMC nanocomposite (MagNPs-CMC) were synthesised and characterised using Braunauer–Emmett–Teller (BET), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and high resolution-transmission electron microscopy (HR-TEM). This study demonstrates the successful application of nanoparticles (NPs) and nanocomposites in the removal of OCPs and heavy metal ions in industrial effluents. The routine assessment and continuous removal become important to attain a state of clean and healthy aquatic ecosystem due to rapid industrial and technological advances.
Extraction and identification of new flavonoid compounds in dandelion Taraxacum mongolicum Hand.-Mazz. with evaluation of antioxidant activities
Authors: Wang Rong , Li Weihua , Fang Cao , Zheng Xinxin , Liu Chao , Huang Qing
Published: February 2023

Due to the interest in the potential pharmacological application of dandelion, the chemical constituents and activities of Taraxacum mongolicum Hand.-Mazz were studied. Box–Behnken response surface methodology was employed to optimize the protocol for extraction of flavonoid from dandelion. The molecular structures of different flavonoid compounds were acquired and analyzed by liquid chromatography-mass spectrometry (LC–MS) and nuclear magnetic resonance (NMR) spectroscopy. Several major flavonoid compounds were isolated and purified, namely, hesperetin-5′-O-β-rhamnoglucoside, hesperetin-7-glucuronide, kaempferol-3-glucoside, baicalein, hyperseroside, which were extracted for the first time from dandelion. Hesperetin-5′-O-β-rhamnoglucoside was identified as a new type of flavonoid that had never reported in the literature. This new flavonoid has outstanding antioxidant activity, as shown by its IC50 value (8.72 mg/L) for scavenging DPPH free radicals. The determination of the structure-related antioxidant activities could be interpreted based on DFT calculations. As such, we have not only illustrated the rich flavonoid contents in Taraxacum mongolicum Hand.-Mazz, but also revealed new types of flavonoid compounds in dandelion in terms of structure and antioxidant properties.
Translating principles of quality control to cardiovascular magnetic resonance: assessing quantitative parameters of the left ventricle in a large cohort
Authors: Riazy Leili , Däuber Sascha , Lange Steffen , Viezzer Darian Steven , Ott Steffen , Wiesemann Stephanie , Blaszczyk Edyta , Mühlberg Fabian , Zange Leonora , Schulz-Menger Jeanette
Published: February 2023

Cardiac magnetic resonance (CMR) examinations require standardization to achieve reproducible results. Therefore, quality control as known as in other industries such as in-vitro diagnostics, could be of essential value. One such method is the statistical detection of long-time drifts of clinically relevant measurements. Starting in 2010, reports from all CMR examinations of a high-volume center were stored in a hospital information system. Quantitative parameters of the left ventricle were analyzed over time with moving averages of different window sizes. Influencing factors on the acquisition and on the downstream analysis were captured. 26,902 patient examinations were exported from the clinical information system. The moving median was compared to predefined tolerance ranges, which revealed an overall of 50 potential quality relevant changes (“alerts”) in SV, EDV and LVM. Potential causes such as change of staff, scanner relocation and software changes were found not to be causal of the alerts. No other influencing factors were identified retrospectively. Statistical quality assurance systems based on moving average control charts may provide an important step towards reliability of quantitative CMR. A prospective evaluation is needed for the effective root cause analysis of quality relevant alerts.
The effects of core stabilization exercises on the neuromuscular function of athletes with ACL reconstruction
Authors: Saki Farzaneh , Shafiee Hossein , Tahayori Behdad , Ramezani Farzaneh
Published: February 2023

Athletes who have undergone anterior cruciate ligament reconstruction (ACLR) often exhibit persistently impaired kinematics and strength. Core stability training appears to be effective for reducing high-risk landing mechanics and preventing primary anterior cruciate ligament (ACL) injuries; however, there have been few attempts to examine their effects in athletes who have undergone ACLR. This study aimed to investigate the effect of eight weeks of simple core stability training on core endurance, hip strength, and knee kinematics in ACLR athletes. Twenty-six male athletes (20–30 years old) with a history of ACL surgery with hamstring tendon autograft were randomly divided into training (n = 13) and control groups (n = 13). The training group performed core stability exercises for eight weeks before starting their team training; the control group did not receive any intervention. Both groups continued their regular team schedule. The core endurance, hip muscle strength, and knee kinematics were assessed by the McGill test, a hand-held dynamometer, and video-taping, respectively. Analysis of covariance test was used for data analysis. The training group showed a significant increase in core endurance, hip abductor and external rotator strength, knee flexion angle, and a significant decrease in the knee valgus angle during single-leg landing in post-training tests compared to their baseline tests (P < 0.05). Our results demonstrated that core stability exercise alters neuromuscular function to a level that is clinically acceptable and statistically significant. Because of the high incidence rate of secondary ACL injury after ACLR, it is recommended that athletes with a history of ACLR benefit from adding core stability exercises to warm-up routines or tertiary prevention programs even after completing post-operative rehabilitation. It is fast and not time-consuming to perform for athletes to reduce the risk factors of re-injury.

Trial registration: This study was registered in the Iranian Registry of Clinical Trials with the number IRCT20190224042827N2, registered on 19 December 2019.
Co-cultivation of Mortierellaceae with Pseudomonas helmanticensis affects both their growth and volatilome
Authors: Probst Maraike , Telagathoti Anusha , Siewert Bianka , Khomenko Iuliia , Betta Emanuela , Biasioli Franco , Peintner Ursula
Published: February 2023

Volatile organic compounds (VOCs) might mediate microbial interactions, especially in spatially structured environments, such as soil. However, the variety and specificity of VOC production are poorly understood. Here, we studied 25 Mortierellaceae strains belonging to the genera Linnemannia and Entomortierella in both pure and co-culture with Pseudomonas helmanticensis under laboratory conditions. We analysed both the fungal growth depending on co-cultivation and the cultures’ volatilomes applying proton-transfer-reaction time-of-flight and gas chromatography-mass spectrometry (PTR-ToF-MS and GC–MS). In a strain-specific manner, we found the fungi’s radial growth rate and colony morphology affected by the presence of P. helmanticensis. The fungus seemed to generally reduce the bacterial growth. The volatilomes of the fungal and bacterial pure and co-cultures were diverse. While the fungi frequently consumed VOCs, P. helmanticensis produced a higher diversity and amount of VOCs than any fungal strain. Our results support that both the pure and co-culture volatilomes are taxonomically conserved. Taken together, our data supports the relevance of VOCs in Mortierellaceae-P. helmanticensis interaction. We also discuss individual VOCs that appear relevant in the interaction.
Room-temperature spin injection from a ferromagnetic semiconductor
Authors: Goel Shobhit , Khang Nguyen Huynh Duy , Osada Yuki , Anh Le Duc , Hai Pham Nam , Tanaka Masaaki
Published: February 2023

Spin injection using ferromagnetic semiconductors at room temperature is a building block for the realization of spin-functional semiconductor devices. Nevertheless, this has been very challenging due to the lack of reliable room-temperature ferromagnetism in well-known group IV and III-V based semiconductors. Here, we demonstrate room-temperature spin injection by using spin pumping in a BiSb/(Ga,Fe)Sb heterostructure, where (Ga,Fe)Sb is a ferromagnetic semiconductor (FMS) with high Curie temperature (TC) and BiSb is a topological insulator (TI). Despite the very small magnetization of (Ga,Fe)Sb at room temperature (45 emu/cc), we detected spin injection from (Ga,Fe)Sb by utilizing the large inverse spin Hall effect (ISHE) in BiSb. Our study provides the first demonstration of spin injection at room temperature from a FMS.
Magnetic hydrogel particles improve nanopore sequencing of SARS-CoV-2 and other respiratory viruses
Authors: Andersen P. , Barksdale S. , Barclay R.A. , Smith N. , Fernandes J. , Besse K. , Goldfarb D. , Barbero R. , Dunlap R. , Jones-Roe T. , Kelly R. , Miao S. , Ruhunusiri C. , Munns A. , Mosavi S. , Sanson L. , Munns D. , Sahoo S. , Swahn O. , Hull K. , White D. , Kolb K. , Noroozi F. , Seelam J. , Patnaik A. , Lepene B.
Published: February 2023

Presented here is a magnetic hydrogel particle enabled workflow for capturing and concentrating SARS-CoV-2 from diagnostic remnant swab samples that significantly improves sequencing results using the Oxford Nanopore Technologies MinION sequencing platform. Our approach utilizes a novel affinity-based magnetic hydrogel particle, circumventing low input sample volumes and allowing for both rapid manual and automated high throughput workflows that are compatible with Nanopore sequencing. This approach enhances standard RNA extraction protocols, providing up to 40 × improvements in viral mapped reads, and improves sequencing coverage by 20–80% from lower titer diagnostic remnant samples. Furthermore, we demonstrate that this approach works for contrived influenza virus and respiratory syncytial virus samples, suggesting that it can be used to identify and improve sequencing results of multiple viruses in VTM samples. These methods can be performed manually or on a KingFisher automation platform.
The effects of sun exposure on colorant identification of permanently and semi-permanently dyed hair
Authors: Holman Aidan , Kurouski Dmitry
Published: February 2023

During bloating and active decay, human remains begin to deform and warp their physical identity. After the skin and muscles loosen and detach from their skeletal structuration, everything but bones, teeth, and hair will fully disintegrate into the soil that surrounds the body. Nearly half of people in the world dye their hair with a variety of permanent and semi-permanent colorants. Expanding upon this, we hypothesized that confirmatory analysis of hair colorants can be used to facilitate and advance forensic analysis of human remains. A growing body of evidence suggests that hair colorants can be identified directly on hair using surface-enhanced Raman spectroscopy (SERS). In this study, we investigate the extent to which SERS can be used to detect black and blue permanent and semi-permanent dyes on hair exposed to sunlight. Our results showed that although substantial photodegradation of all dyes was observed by week 7, SERS enabled highly accurate detection and identification of hair colorants during all 10 weeks of hair exposure to the sunlight with on average 99.2% accuracy. We also found that SERS could be used to predict fading rates of hair colorants. This information can shed light on the exposure of human remains to the exterior environment.
Solubility of palbociclib in supercritical carbon dioxide from experimental measurement and Peng–Robinson equation of state
Authors: Sodeifian Gholamhossein , Hsieh Chieh-Ming , Tabibzadeh Amirmuhammad , Wang Hsu-Chen , Arbab Nooshabadi Maryam
Published: February 2023

Palbociclib is a poorly water-soluble medicine which acts against metastatic breast cancer cells. Among various techniques to improve the solubility of this medicine, applying supercritical technologies to produce micro- and nano-sized particles is a possible option. For this purpose, extraction of solubility data is required. In this research, the solubility of palbociclib in supercritical carbon dioxide (ScCO2) at different equilibrium conditions was measured at temperatures between 308 and 338 K and pressures within 12–27 MPa, for the first time. The minimum and maximum solubility data were found to be 8.1 × 10–7 (at 338 K and 12 MPa) and 2.03 × 10–5 (at 338 K and 27 MPa), respectively. Thereafter, two sets of models, including ten semi-empirical equations and three Peng–Robinson (PR) based integrated models were used to correlate the experimental solubility data. Bian’s model and PR equation of state using van der Waals mixing rules (PR + vdW) showed better accuracy among the examined semi-empirical and integrated models, respectively. Furthermore, the self-consistency of the obtained data was confirmed using two distinct semi-empirical models. At last, the total and vaporization enthalpies of palbociclib solubility in ScCO2 were calculated from correlation results of semi-empirical equations and estimated to be 40.41 and 52.67 kJ/mol, respectively.
Individual attractiveness preferences differentially modulate immediate and voluntary attention
Authors: Roth Tom S. , Samara Iliana , Perea-Garcia Juan Olvido , Kret Mariska E.
Published: February 2023

Physical attractiveness plays a crucial role in mate choice for both men and women. This is reflected in visual attention: people immediately attend towards and look longer at attractive faces, especially when they are motivated to find a partner. However, previous studies did not incorporate real-life dating decisions. Here, we aimed to combine attentional tasks with individual attractiveness ratings and a real-life mate choice context, namely a speed-dating paradigm. We investigated whether heterosexual non-committed young adults showed biases in immediate and voluntary attention towards attractive faces and preferred dating partners. In line with previous research, we found considerable individual differences in individual attractiveness preferences. Furthermore, our results showed that men had a bias towards attractive faces and preferred dating partners in the immediate attention task, while results for women were mixed. In the voluntary attention task, however, both men and women had an attentional bias towards attractive faces and preferred dating partners. Our results suggest that individual attractiveness preferences are good predictors of especially voluntary attention. We discuss these findings from an evolutionary perspective and suggest directions for future research.
Skin irritation in children undergoing orthodontic facemask therapy
Authors: Kim Harim , Kim Jung Suk , Kim Cheol Soon , Becker-Weimann Su Youn , Cha Jung-Yul , Choi Sung-Hwan
Published: February 2023

Orthodontic facemasks are extraoral orthodontic appliances that influence maxillary and mandibular development in children with skeletal Class III malocclusion. While a facemask is most effective in patients before the growth spurt, skin irritation is common during the treatment. Therefore, this retrospective study aimed to investigate the prevalence and pattern of such skin changes and identify their possible associated risk factors. We included 177 patients with skeletal Class III malocclusion who underwent facemask therapy. Patient age and sex, orthodontic parameters expressing the severity of malocclusion, the presence of complaints in the temporomandibular joint (TMJ) areas, and the level of patient cooperation were evaluated. Additionally, the severity and onset time of skin reactions were further analyzed. The results indicated that 43.5% of patients developed skin changes typical of irritant contact dermatitis. Skin irritation was significantly associated with the presence of TMJ complaints and female sex. Furthermore, skin irritation was more common in younger patients. Clinicians should pay special attention to the skin areas that come into contact with the appliance during each follow-up visit to detect potential problems. Moreover, patients and their parents should be given adequate information about the possibility, prevention, and management of skin problems during facemask therapy.
Salinity and high pH reduce denitrification rates by inhibiting denitrifying gene abundance in a saline-alkali soil
Authors: Pan Yongchun , She Dongli , Shi Zhenqi , Cao Taohong , Xia Yongqiu , Shan Jun
Published: February 2023

Denitrification, as the main nitrogen (N) removal process in farmland drainage ditches in coastal areas, is significantly affected by saline-alkali conditions. To elucidate the effects of saline-alkali conditions on denitrification, incubation experiments with five salt and salt-alkali gradients and three nitrogen addition levels were conducted in a saline-alkali soil followed by determination of denitrification rates and the associated functional genes (i.e., nirK/nirS and nosZ Clade I) via N2/Ar technique in combination with qPCR. The results showed that denitrification rates were significantly decreased by 23.83–50.08%, 20.64–57.31% and 6.12–54.61% with salt gradient increasing from 1 to 3‰, 8‰, and 15‰ under 0.05‰, 0.10‰ and 0.15‰ urea addition conditions, respectively. Similarly, denitrification rates were significantly decreased by 44.57–63.24% with an increase of the salt-alkali gradient from 0.5 to 8‰. The abundance of nosZ decreased sharply in the saline condition, while a high salt level significantly decreased the abundance of nirK and nirS. In addition, the increase of nitrogen concentration attenuated the reduction of nirK, nirS and nosZ gene abundance. Partial least squares regression (PLSR) models demonstrated that salinity, dissolved oxygen (DO) in the overlying water, N concentration, and denitrifying gene abundance were key determinants of the denitrification rate in the saline environment, while pH was an additional determinant in the saline-alkali environment. Taken together, our results suggest that salinity and high pH levels decreased the denitrification rates by significantly inhibiting the abundance of the denitrifying genes nirK, nirS, and nosZ, whereas increasing nitrogen concentration could alleviate this effect. Our study provides helpful information on better understanding of reactive N removal and fertilizer application in the coastal areas.