bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Study on Volunteering among Older Korean Immigrants in the United States and Older Koreans in South Korea
Authors: Chang Miya
Published: June 2022

This study aimed to determine the most important factors that influence the prevalence of volunteering among older Koreans in the United States and in Korea and to identify sociodemographic resources, cultural resources, and social resources that are correlated with volunteering. The data were gathered from older Koreans aged 60 or over from the two countries (n = 480). The prevalence of volunteering was 23.3% for older Korean immigrants in the United States versus 14.7% for the older Koreans in Korea. This study found that there was a significant relationship between human capital (individual resources) and volunteering in both countries. Education and financial status had a positive relationship with volunteering among older Koreans in both countries. Cultural resources and social resources were the only important predictors of volunteering among older Korean immigrants in the United States. Regarding types of volunteering, older Koreans in both countries were more likely to participate in informal volunteering than formal volunteering. These findings differ somewhat from those reported by previous studies. This study was the first attempt to examine older adults from these two countries with a focus on the correlation between cultural factors, social resources, and volunteering.
Can Public–Private Partnership Wastewater Treatment Projects Help Reduce Urban Sewage Disposal? Empirical Evidence from 267 Cities in China
Authors: Hou Xinshuo
Published: June 2022

Human activities have placed enormous pressure on the world’s water resources. To improve the efficiency of water supply and wastewater treatment, public–private partnerships (PPPs) are widely used for sewage treatment. However, an academic question remains about whether PPP sewage treatment projects (PPPSTs) help reduce urban sewage disposal when responsibilities shift from the public sector to the private sector. This study used panel data of 267 prefecture-level cities in China from 2009 to 2020 to construct a difference-in-difference (DID) model based on the counterfactual framework to answer this question empirically. The model results significantly support the effect of PPPSTs on sewage disposal reduction. Furthermore, these results passed the parallel trend test and the placebo test, and the results were still achieved when the quadratic term of the core variable was introduced, indicating that the model is reliable. In addition, the moderating effect models were used to expand the analysis. That is, the regressions were derived by multiplying the relevant extended variables and the core independent variables. This analysis indicates that the operation mode of PPPST and the characteristics of national demonstration play an essential role in reducing the amount of urban sewage disposal. However, the effect of fiscal decentralization is not apparent. These conclusions were also confirmed in the model using the investment scale of PPPSTs. Therefore, paying attention to the formation of PPPST contracts and adopting a practical supervision system is of great significance for improving the effect of sewage disposal reduction.
Medical Photography Usage Amongst Doctors at a Portuguese Hospital
Authors: Cura Mariana , Alves Hélio , Andrade José Paulo
Published: June 2022

Technological advancements in smartphones have made it possible to create high-quality medical photographs, with the potential to revolutionise patient care. To ensure the security of the patient’s data, it is important that medical professionals receive informed consent from the patient, that physical conditions are met to take a photograph, and that these medical images are stored correctly. This study aimed to determine if medical professionals of an academic hospital make use of medical photography, and how the content is obtained, stored, transferred, and used. Methods: A 30-question questionnaire was distributed across 29 medical departments at Centro Hospitalar Universitário de São João (CHUSJ), a tertiary referral and teaching hospital in Porto, Portugal, with approximately 900 medical professionals. Quantitative statistical methods were used to analyse questionnaire responses. Results: There were a total of 257 respondents. Of these, 93% used medical photography, 70% used it to document a patient’s clinical progress, 70% to ask for a second opinion, 56% for education, 65% for research and publication, and 68% to present at medical conferences. Medical photography was used by 33% weekly and 36% monthly, with 71% of respondents always asking for the patients’ consent before taking a photograph. Doctors aged 20–40 years used photography more often than doctors over 40 years of age to document the clinical progress of the patients (77% and 52%, respectively, p = 0.01) and to ask for a second opinion (78% and 52%, respectively, p < 0.001). Conclusions: Our study shows that medical photography is a common practice amongst medical doctors. However, appropriate measures need to be created to obtain patients’ consent, store images, and sure the security of patients’ information.
Potential Therapeutic Candidates against Chlamydia pneumonia Discovered and Developed In Silico Using Core Proteomics and Molecular Docking and Simulation-Based Approaches
Authors: Kadi Roqayah H. , Altammar Khadijah A. , Hassan Mohamed M. , Shater Abdullah F. , Saleh Fayez M. , Gattan Hattan , Al-ahmadi Bassam M. , AlGabbani Qwait , Mohammedsaleh Zuhair M.
Published: June 2022

Chlamydia pneumonia, a species of the family Chlamydiacea, is a leading cause of pneumonia. Failure to eradicate C. pneumoniae can lead to chronic infection, which is why it is also considered responsible for chronic inflammatory disorders such as asthma, arthritis, etc. There is an urgent need to tackle the major concerns arising due to persistent infections caused by C. pneumoniae as no FDA-approved drug is available against this chronic infection. In the present study, an approach named subtractive proteomics was employed to the core proteomes of five strains of C. pneumonia using various bioinformatic tools, servers, and software. However, 958 non-redundant proteins were predicted from the 4754 core proteins of the core proteome. BLASTp was used to analyze the non-redundant genes against the proteome of humans, and the number of potential genes was reduced to 681. Furthermore, based on subcellular localization prediction, 313 proteins with cytoplasmic localization were selected for metabolic pathway analysis. Upon subsequent analysis, only three cytoplasmic proteins, namely 30S ribosomal protein S4, 4-hydroxybenzoate decarboxylase subunit C, and oligopeptide binding protein, were identified, which have the potential to be novel drug target candidates. The Swiss Model server was used to predict the target proteins’ three-dimensional (3D) structure. The molecular docking technique was employed using MOE software for the virtual screening of a library of 15,000 phytochemicals against the interacting residues of the target proteins. Molecular docking experiments were also evaluated using molecular dynamics simulations and the widely used MM-GBSA and MM-PBSA binding free energy techniques. The findings revealed a promising candidate as a novel target against C. pneumonia infections.
Alzheimer’s Disease Association with Metals and Metalloids Concentration in Blood and Urine
Authors: Strumylaite Loreta , Kregzdyte Rima , Kucikiene Odeta , Baranauskiene Dale , Simakauskiene Vaida , Naginiene Rima , Damuleviciene Gyte , Lesauskaite Vita , Zemaitiene Reda
Published: June 2022

As there is some evidence that the risk for Alzheimer’s disease (AD) is partially attributable to environmental exposure to some metals and metalloids, we examined an association between AD and arsenic, chromium, and selenium in 53 AD patients and 217 controls. Urinary arsenic, blood chromium, and selenium were determined by inductively coupled plasma mass spectrometry. Logistic regression models calculating odds ratios (ORs) and 95% confidence intervals (CI) were used to estimate AD association with arsenic, chromium, and selenium. In AD patients, urinary arsenic and blood chromium were significantly higher, while blood selenium was significantly lower compared to controls. Increased blood selenium was related to a significant decrease in the odds of AD after adjustment for risk factors. Blood selenium per 1 kg × 10−9/m3 × 10−4 increment was associated with 1.4 times lower risk of AD (OR = 0.71; 95% CI 0.58–0.87). A significant increase in the odds of AD associated with increased blood chromium was also seen in the adjusted model: the OR per 1 kg × 10−9/m3 × 10−3 chromium increment was 2.39 (95% CI 1.32–4.31). The association of urinary arsenic with the risk of AD was not significant. The data obtained provide evidence that selenium reduces the risk of Alzheimer’s disease, while chromium increases it.
Effective and Acceptable Eco-Driving Guidance for Human-Driving Vehicles: A Review
Authors: Tu Ran , Xu Junshi , Li Tiezhu , Chen Haibo
Published: June 2022

Eco-driving guidance refers to courses, warnings, or suggestions provided to human drivers to improve driving behaviour to enable less energy use and emissions. This paper reviews existing eco-driving guidance studies and identifies challenges to tackle in the future. We summarize two categories of current guidance systems, static and dynamic, distinguished by whether real-world driving records are used to generate behaviour guidance or not. We find that influencing factors, such as the content of suggestions, the display methods, and drivers’ socio-demographic characteristics, have varied effects on the guidance results across studies. Drivers are reported to have basic eco-driving knowledge, while the question of how to motivate the acceptance and practice of such behaviour, especially in the long term, is overlooked. Adaptive driving suggestions based on drivers’ individual habits can improve the effectiveness and acceptance while this field is under investigation. In-vehicle assistance presents potential safety issues, and visualized in-vehicle assistance is reported to be most distractive. Given existing studies focusing on the operational level, a common agreement on the guidance design and associated influencing factors has yet to be reached. Research on the systematic and tactical design of eco-driving guidance and in-vehicle interaction is advised.
Dynamics of Household Waste Segregation Behaviour in Urban Community in Ujjain, India: A Framework Analysis
Authors: Sahoo Krushna Chandra , Soni Rachna , Kalyanasundaram Madhanraj , Singh Surya , Parashar Vivek , Pathak Ashish , Purohit Manju R. , Sabde Yogesh , Stålsby Lundborg Cecilia , Sidney Annerstedt Kristi , Atkins Salla , Rousta Kamran , Diwan Vishal
Published: June 2022

Waste segregation practices must be socially acceptable, affordable, context-specific, and participatory, which is essential for promoting waste segregation. Therefore, this study explored the urban community members’ motivation, opportunity, and household waste segregation ability. We performed a qualitative study in Ujjain city, India. Ten focus group discussions and eight in-depth interviews were conducted with female and male household members in residential and slum areas. All interviews were digitally recorded, transcribed, and translated. We used the thematic framework technique using the Motivation-Opportunity-Ability-Behaviour theory for analysis. Three themes were constructed: motivation, where household members are motivated to sort waste yet fear the consequences of improper sorting; ability, where household waste segregation is rapidly gaining acceptance as a social norm; and opportunities, involving convenient facilities and a social support system for household members towards waste segregation. This study contributes to developing a knowledge base on waste segregation behaviour and a repertoire to facilitate evidence-based management and policymaking. There is a need for educational intervention and women’s self-help groups’ involvement to develop community orientation and waste segregation literacy. Finally, this study emphasizes the importance of all three behavioural change components, i.e., motivation, opportunity, and ability, in managing sustainable waste segregation practices.
The State of the Art of Piezo1 Channels in Skeletal Muscle Regeneration
Authors: Bernareggi Annalisa , Bosutti Alessandra , Massaria Gabriele , Giniatullin Rashid , Malm Tarja , Sciancalepore Marina , Lorenzon Paola
Published: June 2022

Piezo1 channels are highly mechanically-activated cation channels that can sense and transduce the mechanical stimuli into physiological signals in different tissues including skeletal muscle. In this focused review, we summarize the emerging evidence of Piezo1 channel-mediated effects in the physiology of skeletal muscle, with a particular focus on the role of Piezo1 in controlling myogenic precursor activity and skeletal muscle regeneration and vascularization. The disclosed effects reported by pharmacological activation of Piezo1 channels with the selective agonist Yoda1 indicate a potential impact of Piezo1 channel activity in skeletal muscle regeneration, which is disrupted in various muscular pathological states. All findings reported so far agree with the idea that Piezo1 channels represent a novel, powerful molecular target to develop new therapeutic strategies for preventing or ameliorating skeletal muscle disorders characterized by an impairment of tissue regenerative potential.
Bio-Based Polyurethane and Its Composites towards High Damping Properties
Authors: Hu Shikai , Wu Yaowen , Fu Guoqing , Shou Tao , Zhai Mengyao , Yin Dexian , Zhao Xiuying
Published: June 2022

The operation of mechanical equipment inevitably generates vibrations and noise, which are harmful to not only the human body but also to the equipment in use. Damping materials, which can convert mechanical energy into thermal energy, possess excellent damping properties in the glass transition region and can alleviate the problems caused by vibration and noise. However, these materials mainly rely on petroleum-based resources, and their glass transition temperatures (Tg) are lower than room temperature. Therefore, bio-based materials with high damping properties at room temperature must be designed for sustainable development. Herein, we demonstrate the fabrication of bio-based millable polyurethane (BMPU)/hindered phenol composites that could overcome the challenges of sustainable development and exhibit high damping properties at room temperature. BMPUs with a high Tg were prepared from modified poly (lactic acid)-based polyols, the unsaturated chain extender trimethylolpropane diallylether, and 4,4′-diphenylmethane diisocyanate, and 3,9-Bis-{1,1-dimethyl-2[β-(3-tert-butyl-4-hydroxy-5-methylphenyl-)propionyloxy]ethyl}-2,4,8,10-tetraoxaspiro [5,5]-undecane (AO-80) was added to prepare BMPU/AO-80 composites. Finally, the properties of the BMPUs and BMPU/AO-80 composites were systematically evaluated. After adding 30 phr of AO-80, the Tg and maximum loss factor (tan δmax) of BMPU/AO-80 composites increased from 7.8 °C to 13.5 °C and from 1.4 to 2.0, respectively. The tan δmax showed an improvement of 43%. Compared with other polyurethanes, the prepared BMPU/AO-80 composites exhibited higher damping properties at room temperature. This study proposes a new strategy to reduce society’s current dependence on fossil resources and design materials featuring high damping properties from sustainable raw materials.
Novel Insights on Dietary Polyphenols for Prevention in Early-Life Origins of Hypertension: A Review Focusing on Preclinical Animal Models
Authors: Tain You-Lin , Hsu Chien-Ning
Published: June 2022

Polyphenols are the largest group of phytochemicals with health benefits. Early life appears to offer a critical window of opportunity for launching interventions focused on preventing hypertension, as increasing evidence supports the supposition that hypertension can originate in early life. Although polyphenols have antihypertensive actions, knowledge of the potential beneficial action of the early use of polyphenols to avert the development of hypertension is limited. Thus, in this review, we first provide a brief summary of the chemistry and biological function of polyphenols. Then, we present the current epidemiological and experimental evidence supporting the early-life origins of hypertension. We also document animal data on the use of specific polyphenols as an early-life intervention to protect offspring against hypertension in adulthood and discuss underlying mechanisms. Continued research into the use of polyphenols to prevent hypertension from starting early in life will have far-reaching implications for future health.
OsASR6 Alleviates Rice Resistance to Xanthomonas oryzae via Transcriptional Suppression of OsCIPK15
Authors: Guo Weiyi , Chen Songyu , Xu Youping , Cai Xinzhong
Published: June 2022

The plant-specific ASR (abscisic acid, stress and ripening) transcription factors are pivotal regulators of plant responses to abiotic stresses. However, their functions in plant disease resistance remain largely unknown. In this study, we revealed the role of OsASR6 in rice plants’ resistance to two important bacterial diseases caused by Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc) and elucidated the mechanisms underlying OsASR6-regulated resistance. The expression of OsASR6 was strongly elevated in response to both Xoo and Xoc challenges. Silencing of OsASR6 in OsASR6-RNAi transgenic plants markedly enhanced rice resistance to the two bacterial pathogens. Moreover, comparative transcriptome analyses for OsASR6-RNAi and wild-type plants inoculated and uninoculated with Xoc demonstrated that OsASR6 suppressed rice resistance to Xoc by comprehensively fine-tuning CIPK15- and WRKY45-1-mediated immunity, SA signaling and redox homeostasis. Further luciferase reporter assays confirmed that OsASR6 negatively regulated CIPK15 but not WRKY45-1 expression in planta. Overexpression of OsCIPK15 strongly enhanced rice resistance to Xoo and Xoc. Collectively, these results reveal that OsASR6 alleviates rice resistance through the transcriptional suppression of OsCIPK15, and thus links calcium signaling to rice resistance against X. oryzae. Our findings provide insight into the mechanisms underlying OsASR6-mediated regulation of rice resistance to X. oryzae.
The Role of Non-Coding RNAs in Kidney Diseases
Authors: Metzinger Laurent , Moreno Juan Antonio , Metzinger-Le Meuth Valérie
Published: June 2022

Renal diseases include different pathologies, such as acute kidney injury (AKI), chronic kidney disease (CKD), end-stage renal disease (ESRD), diabetic nephropathy (DN), kidney cancer, polycystic kidney disease, etc [...]
Novel Targets for Old and Diseased Hearts
Authors: Rurali Erica , Pompilio Giulio , Zacchigna Serena
Published: June 2022

In this Special Issue we cover a selection of original articles and reviews devoted to the definition of novel molecular targets in cardiovascular diseases, which not only deepen our knowledge on the pathogenesis of the diseases under study, but potentially pave the way to novel diagnostic tools and therapeutic approaches [...]
Ferulic Acid, Pterostilbene, and Tyrosol Protect the Heart from ER-Stress-Induced Injury by Activating SIRT1-Dependent Deacetylation of eIF2α
Authors: Monceaux Kévin , Gressette Mélanie , Karoui Ahmed , Pires Da Silva Julie , Piquereau Jérôme , Ventura-Clapier Renée , Garnier Anne , Mericskay Mathias , Lemaire Christophe
Published: June 2022

Disturbances in Endoplasmic Reticulum (ER) homeostasis induce ER stress, which has been involved in the development and progression of various heart diseases, including arrhythmias, cardiac hypertrophy, ischemic heart diseases, dilated cardiomyopathy, and heart failure. A mild-to-moderate ER stress is considered beneficial and adaptative for heart functioning by engaging the pro-survival unfolded protein response (UPR) to restore normal ER function. By contrast, a severe or prolonged ER stress is detrimental by promoting cardiomyocyte apoptosis through hyperactivation of the UPR pathways. Previously, we have demonstrated that the NAD+-dependent deacetylase SIRT1 is cardioprotective in response to severe ER stress by regulating the PERK pathway of the UPR, suggesting that activation of SIRT1 could protect against ER-stress-induced cardiac damage. The purpose of this study was to identify natural molecules able to alleviate ER stress and inhibit cardiomyocyte cell death through SIRT1 activation. Several phenolic compounds, abundant in vegetables, fruits, cereals, wine, and tea, were reported to stimulate the deacetylase activity of SIRT1. Here, we evaluated the cardioprotective effect of ten of these phenolic compounds against severe ER stress using cardiomyoblast cells and mice. Among the molecules tested, we showed that ferulic acid, pterostilbene, and tyrosol significantly protect cardiomyocytes and mice heart from cardiac alterations induced by severe ER stress. By studying the mechanisms involved, we showed that the activation of the PERK/eIF2α/ATF4/CHOP pathway of the UPR was reduced by ferulic acid, pterostilbene, and tyrosol under ER stress conditions, leading to a reduction in cardiomyocyte apoptosis. The protection afforded by these phenolic compounds was not directly related to their antioxidant activity but rather to their ability to increase SIRT1-mediated deacetylation of eIF2α. Taken together, our results suggest that ferulic acid, pterostilbene, and tyrosol are promising molecules to activate SIRT1 to protect the heart from the adverse effects of ER stress.
Kinase Activity of PAR1b, Which Mediates Nuclear Translocation of the BRCA1 Tumor Suppressor, Is Potentiated by Nucleic Acid-Mediated PAR1b Multimerization
Authors: Nishikawa Hiroko , Christiany Priscillia , Hayashi Takeru , Iizasa Hisashi , Yoshiyama Hironori , Hatakeyama Masanori
Published: June 2022

PAR1b is a cytoplasmic serine/threonine kinase that controls cell polarity and cell–cell interaction by regulating microtubule stability while mediating cytoplasmic-to-nuclear translocation of BRCA1. PAR1b is also a cellular target of the CagA protein of Helicobacter pylori, which leads to chronic infection causatively associated with the development of gastric cancer. The CagA-PAR1b interaction inactivates the kinase activity of PAR1b and thereby dampens PAR1b-mediated BRCA1 phosphorylation, which reduces the level of nuclear BRCA1 and thereby leads to BRCAness and BRCAness-associated genome instability underlying gastric carcinogenesis. While PAR1b can multimerize within the cells, little is known about the mechanism and functional role of PAR1b multimerization. We found in the present study that PAR1b was multimerized in vitro by binding with nucleic acids (both single- and double-stranded DNA/RNA) via the spacer region in a manner independent of nucleic-acid sequences, which markedly potentiated the kinase activity of PAR1b. Consistent with these in vitro observations, cytoplasmic introduction of double-stranded DNA or expression of single-stranded RNA increased the PAR1b kinase activity in the cells. These findings indicate that the cytoplasmic DNA/RNA contribute to nuclear accumulation of BRCA1 by constitutively activating/potentiating cytoplasmic PAR1b kinase activity, which is subverted in gastric epithelial cells upon delivery of H. pylori CagA oncoprotein.
Vascularization of Cell-Laden Microfibres by Femtosecond Laser Processing
Authors: Verit Isabel , Gemini Laura , Preterre Julie , Pfirmann Pierre , Bakis Hugo , Fricain Jean-Christophe , Kling Rainer , Rigothier Claire
Published: June 2022

To face the increasing demand for organ transplantation, currently the development of tissue engineering appears as the best opportunity to effectively regenerate functional tissues and organs. However, these approaches still face the lack of an efficient method to produce an efficient vascularization system. To answer these issues, the formation of an intra-volume channel within a three-dimensional, scaffold free, mature, and cell-covered collagen microfibre is here investigated through laser-induced cavitation. An intra-volume channel was formed upon irradiation with a near-infrared, femtosecond laser beam, focused with a high numerical aperture lens. The laser beam directly crossed the surface of a dense and living-cell bilayer and was focused behind the bilayer to induce channel formation in the hydrogel core while preserving the cell bilayer. Channel formation was assessed through confocal microscopy. Channel generation inside the hydrogel core was enhanced by the formation of voluminous cavitation bubbles with a lifetime longer than 30 s, which also improved intra-volume channel durability. Twenty-four hours after laser processing, cellular viability dropped due to a lack of sufficient hydration for processing longer than 10 min. However, the processing automation could drastically reduce the cellular mortality, this way enabling the formation of hollowed microfibres with a high density of living-cell outer bilayer.
Beta-Site Amyloid Precursor Protein-Cleaving Enzyme Inhibition Partly Restores Sevoflurane-Induced Deficits on Synaptic Plasticity and Spine Loss
Authors: Wang Xingxing , Shi Qinfang , Pradhan Arpit Kumar , Ziegon Laura , Schlegel Martin , Rammes Gerhard
Published: June 2022

Evidence indicates that inhalative anesthetics enhance the β-site amyloid precursor protein (APP)-cleaving enzyme (BACE) activity, increase amyloid beta 1-42 (Aβ1–42) aggregation, and modulate dendritic spine dynamics. However, the mechanisms of inhalative anesthetics on hippocampal dendritic spine plasticity and BACE-dependent APP processing remain unclear. In this study, hippocampal slices were incubated with equipotent isoflurane (iso), sevoflurane (sevo), or xenon (Xe) with/without pretreatment of the BACE inhibitor LY2886721 (LY). Thereafter, CA1 dendritic spine density, APP processing-related molecule expressions, nectin-3 levels, and long-term potentiation (LTP) were tested. The nectin-3 downregulation on LTP and dendritic spines were evaluated. Sevo treatment increased hippocampal mouse Aβ1–42 (mAβ1–42), abolished CA1-LTP, and decreased spine density and nectin-3 expressions in the CA1 region. Furthermore, CA1-nectin-3 knockdown blocked LTP and reduced spine density. Iso treatment decreased spine density and attenuated LTP. Although Xe blocked LTP, it did not affect spine density, mAβ1–42, or nectin-3. Finally, antagonizing BACE activity partly restored sevo-induced deficits. Taken together, our study suggests that sevo partly elevates BACE activity and interferes with synaptic remodeling, whereas iso mildly modulates synaptic changes in the CA1 region of the hippocampus. On the other hand, Xe does not alternate dendritic spine remodeling.
In Vitro Selection and Characterization of HIV-1 Variants with Increased Resistance to LP-40, Enfuvirtide-Based Lipopeptide Inhibitor
Authors: Hu Yue , Yu Wenjiang , Geng Xiuzhu , Zhu Yuanmei , Chong Huihui , He Yuxian
Published: June 2022

In our previous work, we replaced the TRM (tryptophan-rich motif) of T20 (Enfuvirtide) with fatty acid (C16) to obtain the novel lipopeptide LP-40, and LP-40 displayed enhanced antiviral activity. In this study, we investigated whether the C16 modification could enhance the high-resistance barrier of the inhibitor LP-40. To address this question, we performed an in vitro simultaneous screening of HIV-1NL4-3 resistance to T20 and LP-40. The mechanism of drug resistance for HIV-1 Env was further studied using the expression and processing of the Env glycoprotein, the effect of the Env mutation on the entry and fusion ability of the virus, and an analysis of changes to the gp41 core structure. The results indicate that the LP-40 activity is enhanced and that it has a high resistance barrier. In a detailed analysis of the resistance sites, we found that mutations in L33S conferred a stronger resistance, except for the well-recognized mutations in amino acids 36–45 of gp41 NHR, which reduced the inhibitory activity of the CHR-derived peptides. The compensatory mutation of eight amino acids in the CHR region (NDQEEDYN) plays an important role in drug resistance. LP-40 and T20 have similar resistance mutation sites, and we speculate that the same resistance profile may arise if LP-40 is used in a clinical setting.
Identification and Defensive Characterization of PmCYP720B11v2 from Pinus massoniana
Authors: Liu Bin , Xie Yini , Yin Huanhuan , Zhou Zhichun , Liu Qinghua
Published: June 2022

Pinus massoniana is a pioneer species for afforestation timber and oleoresin, while epidemics of pinewood nematode (PWN; Bursaphelenchus xylophilus) are causing a serious biotic disaster for P. massoniana in China. Importantly, resistant P. massoniana could leak copious oleoresin terpenoids to build particular defense fronts for survival when attacked by PWN. However, the defense mechanisms regulating this process remain unknown. Here, PmCYP720B11v2, a cytochrome P450 monooxygenase gene, was first identified and functionally characterized from resistant P. massoniana following PWN inoculation. The tissue-specific expression pattern and localization of PmCYP720B11v2 at the transcript and protein levels in resistant P. massoniana indicated that its upregulation in the stem supported its involvement in the metabolic processes of diterpene biosynthesis as a positive part of the defense against PWN attack. Furthermore, overexpression of PmCYP720B11v2 may enhance the growth and development of plants. In addition, PmCYP720B11v2 activated the metabolic flux of antioxidases and stress-responsive proteins under drought conditions and improved drought stress tolerance. Our results provide new insights into the favorable role of PmCYP720B11v2 in diterpene defense mechanisms in response to PWN attack in resistant P. massoniana and provide a novel metabolic engineering scenario to reform the stress tolerance potential of tobacco.
Cationic Surfactants as Disinfectants against SARS-CoV-2
Authors: Karamov Eduard V. , Larichev Viktor F. , Kornilaeva Galina V. , Fedyakina Irina T. , Turgiev Ali S. , Shibaev Andrey V. , Molchanov Vyacheslav S. , Philippova Olga E. , Khokhlov Alexei R.
Published: June 2022

The virucidal activity of a series of cationic surfactants differing in the length and number of hydrophobic tails (at the same hydrophilic head) and the structure of the hydrophilic head (at the same length of the hydrophobic n-alkyl tail) was compared. It was shown that an increase in the length and number of hydrophobic tails, as well as the presence of a benzene ring in the surfactant molecule, enhance the virucidal activity of the surfactant against SARS-CoV-2. This may be due to the more pronounced ability of such surfactants to penetrate and destroy the phospholipid membrane of the virus. Among the cationic surfactants studied, didodecyldimethylammonium bromide was shown to be the most efficient as a disinfectant, its 50% effective concentration (EC50) being equal to 0.016 mM. Two surfactants (didodecyldimethylammonium bromide and benzalkonium chloride) can deactivate SARS-CoV-2 in as little as 5 s.
Nanocarrier-Loaded Imidaclothiz Promotes Plant Uptake and Decreases Pesticide Residue
Authors: Jiang Qinhong , Peng Min , Yin Meizhen , Shen Jie , Yan Shuo
Published: June 2022

There is a great demand for improving the effective utilization of pesticides and reducing their application for sustainable agriculture, and polymeric nanoparticles have provided strong technical support for the efficient delivery of pesticides. To this context, we tried to construct a relatively safe imidaclothiz nano-delivery system for enhanced plant uptake, reduced pesticide residue and improved bioactivity toward green peach aphids. The imidaclothiz could be assembled with the hydrophobic core of SPc through hydrophobic association, which led to the self-assembly of nanoscale imidaclothiz/SPc complex consisting of nearly spherical particles. The SPc decreased the contact angle of imidaclothiz drops and remarkably increased the plant uptake. Furthermore, the bioactivity and control efficacy of imidaclothiz were significantly improved with the help of SPc in both laboratory and field. Excitingly, the residue of imidaclothiz decreased with the help of SPc 7 d after the treatment due to the faster degradation of nanoscale imidaclothiz/SPc complex, which exhibited no negative effects on agronomic traits of tobacco plants. The current study successfully constructed a nano-delivery system for imidaclothiz, which can not only increase the effective utilization of pesticides, but also decrease the pesticide residue.
Molecular Mechanisms of Changes in Homeostasis of the Dermal Extracellular Matrix: Both Involutional and Mediated by Ultraviolet Radiation
Authors: Zorina Alla , Zorin Vadim , Kudlay Dmitry , Kopnin Pavel
Published: June 2022

Skin aging is a multi-factorial process that affects nearly every aspect of skin biology and function. With age, an impairment of structures, quality characteristics, and functions of the dermal extracellular matrix (ECM) occurs in the skin, which leads to disrupted functioning of dermal fibroblasts (DFs), the main cells supporting morphofunctional organization of the skin. The DF functioning directly depends on the state of the surrounding collagen matrix (CM). The intact collagen matrix ensures proper adhesion and mechanical tension in DFs, which allows these cells to maintain collagen homeostasis while ECM correctly regulates cellular processes. When the integrity of CM is destroyed, mechanotransduction is disrupted, which is accompanied by impairment of DF functioning and destruction of collagen homeostasis, thereby contributing to the progression of aging processes in skin tissues. This article considers in detail the processes of skin aging and associated changes in the skin layers, as well as the mechanisms of these processes at the molecular level.
The Effects of Graphene Oxide-Silica Nano-Hybrid Materials on the Rheological Properties, Mechanical Properties, and Microstructure of Cement-Based Materials
Authors: Long Zizhi , Chen Youzhi , Yin Weisong , Wu Xiuqi , Wang Yun
Published: June 2022

Despite their excellent performance, two-dimension nanomaterials have certain limitations in improving the performance of cement-based materials due to their poor dispersity in the alkaline environment. This paper has synthesized a new two-dimension stacked GO-SiO2 (GOS) hybrid through the sol-gel method. Nano-SiO2 is coated on the surface of GO with wrinkling characteristics, and the atomic ratio of C, O, and Si in GOS is 1:1.69:0.57. The paper discusses the impacts on the spreading, Marsh cone flow time, rheological properties, mechanical properties, and microstructure of cement-based materials for the GOS at different mixing quantities. Furthermore, with the same mixing quantity of 0.01%, the influences on the dispersity, flow properties, rheological parameters, and mechanical properties of GOS and graphene oxide (GO) are compared. Lastly, fuzzy matrix analysis has been adopted to analyze the comprehensive performance of cement-based materials containing GOS. The research results indicate that, compared with the reference sample, the spreading for the GOS cement mortar with 0.01% mixing quantity was reduced by 4.76%, the yield shear stress increased by 37.43%, and the equivalent plastic viscosity was elevated by 2.62%. In terms of the 28 d cement pastes, the compressive and flexural strength were boosted by 27.17% and 42.86%, respectively. According to the optical observation, GOS shows better dispersion stability in the saturated calcium hydroxide solution and simulated pore solution than GO. Compared with the cement-based materials with the same mixing quantity (0.01%), GOS has higher spreading, lower shear yield stress, and higher compressive and flexural strength than GO. Finally, according to the results of fuzzy matrix analysis, when the concentration of GOS is 0.01%, it presents a more excellent comprehensive performance with the highest score. Among the performance indicators, the most significant improvement was in the flexural properties of cement-based materials, which increased from 8.6 MPa to 12.3 MPa on the 28 d.
The Role of Al-10%Si Coating in the Manufacture and Use of Aluminized Open-Joint Steel Tubes
Authors: Żaba Krzysztof , Trzepieciński Tomasz
Published: June 2022

The paper presents the results of laboratory and industrial tests on the role of Al-10%Si coatings in the manufacture and use of welded aluminized steel tubes. The tubes were fabricated from DX53D + AS120 steel tubes coated with Al-10%Si coating. Investigations were carried out on individual processes in the manufacture of welded tubes aimed at determining the effect of the coating properties on the conditions of the forming process and vice versa. In the next step, a quality assessment has been conducted on the finished tubes. Then, selected tests simulating the operating conditions of tubes used for the elements of exhaust systems are presented. Analyses of the susceptibility of strips to plastic deformation and evaluation of the adhesion of the Al-10%Si coating to the steel base metal were carried out using bending tests. From the results, it was proved that the Al-10%Si coating determines the technical features of the workpiece material, affects the manufacturing process, and determines the quality of aluminized steel tubes. The quality of the coating surface depended on the annealing temperature and annealing time. The higher the temperature, the shorter the time needed to produce a change in the coating properties. A p-value reported from a tests is less than 0.05.
Use of Natural Zeolite and Glass Powder Mixture as Partial Replacement of Portland Cement: The Effect on Hydration, Properties and Porosity
Authors: Kriptavičius Dalius , Girskas Giedrius , Skripkiūnas Gintautas
Published: June 2022

The study investigates effect of the additive consisting of natural zeolite (clinoptilolite) and soda lime glass powder on the hydration, mechanical properties and porosity of Portland cement concrete. The effect of mineral additive on the technological, physical-mechanical properties and porosity of the mortar was investigated by increasing the content of natural zeolite and glass powder added to the mortar up to 20% by weight of cement in increments of 5% and different particles size of natural zeolite. The mixes with the best technological and mechanical properties were identified and further studies were conducted by replacing 10% and 15% of cement with natural zeolite and soda lime glass with an average grain size of 59.3 μm, 29.0 μm or 3.6 μm of zeolite, and 29.6 μm of glass powder. The hydration process and microstructure of hardened cement paste modified with the aforementioned mineral additives was analysed by microcalorimetry, X-ray diffraction tests and thermogravimetric analysis. The optimal composition of cement paste and particle size distribution of natural zeolite were determined to achieve the higher flexural and compressive strength and lower open porosity. The mixture of mineral additives has the highest effect in terms of flexural and compressive strength and open porosity when added at the proportion 75:15:10 (cement:natural zeolite:soda lime glass) and when zeolite with an average particle size of about 3.6 μm is used
Plasma Electrolytic Polishing—An Ecological Way for Increased Corrosion Resistance in Austenitic Stainless Steels
Authors: Zatkalíková Viera , Podhorský Štefan , Štrbák Milan , Liptáková Tatiana , Markovičová Lenka , Kuchariková Lenka
Published: June 2022

Plasma electrolytic polishing (PEP) is an environment-friendly alternative to the conventional electrochemical polishing (EP), giving optimal surface properties and improved corrosion resistance with minimum energy and time consumption, which leads to both economic and environmental benefits. This paper is focused on the corrosion behavior of PEP treated AISI 316L stainless steel widely used as a biomaterial. Corrosion resistance of plasma electrolytic polished surfaces without/with chemical pretreatment (acid cleaning) is evaluated and compared with original non-treated (as received) surfaces by three independent test methods: electrochemical impedance spectroscopy (EIS), potentiodynamic polarization (PP), and exposure immersion test. All corrosion tests are carried out in the 0.9 wt.% NaCl solution at a temperature of 37 ± 0.5 °C to simulate the internal environment of a human body. The quality of tested surfaces is also characterized by optical microscopy and by the surface roughness parameters. The results obtained indicated high corrosion resistance of PEP treated surfaces also without chemical pretreatment, which increases the ecological benefits of PEP technology.
Tensile Properties of Additively Manufactured Thermoplastic Composites Reinforced with Chopped Carbon Fibre
Authors: Majko Jaroslav , Vaško Milan , Handrik Marián , Sága Milan
Published: June 2022

3D printing allows controlled deposition of composite components, which the user defines by the modification of the printing parameters. The article demonstrates that all observed printing parameters (infill type, infill orientation) influence the tensile test results of nylon reinforced with chopped carbon fiber. The highest tensile strength obtains specimens with the maximum number of walls around the circumference. The plastic region of the tensile diagram differs significantly with the change of material orientation in the structure, as the specimens with material deposited 45/−45 to the load axis have four times greater tensile strains and 20% higher tensile stresses than 0/90. The assessment of results reveals the significant difference between deformations at break and permanent deformations. In addition, the permanent lateral strain reaches up to 20%. Finally, the article consists of a brief assessment of the printing parameters (printing time, weight) of individual series. The future modelling in FEA software requires additional experiments to verify the viscoelastic properties of the material.
Sensitivity Analysis of Reinforced Aluminum Based Metal Matrix Composites
Authors: Gillani Fouzia , Khan Muhammad Zubair , Shah Owaisur Rahman
Published: June 2022

Metal matrix composites (MMCs) have wide applications due to being lightweight, their high strength, and immense resistance to wear. To explore new generation materials like aluminum-based metal matrix composites (AMCs) for wide engineering applications, the present work aimed at investigating the effect of changes in composition, sintering time, and temperature on the hardness and surface roughness of AMCs containing SiC and ZrSiO4 in wt % of 5, 20, 30, and 40 binary and hybrid sample pallets. The samples have been prepared by powder metallurgy (PM) method under 1000 psi pressure. After compaction, the above pallets sintered at different temperatures ranging from 500 °C to 1100 °C with an increment of 200 °C and 15 min intervals for four levels of temperature and time, respectively. Afterwards, sensitivity analysis has been done by investigating the effect of chemical composition, sintering time, and sintering temperature of the binary and hybrid composites on hardness and surface roughness. Morphological studies on the composites were carried out using field emission scanning electron microscope (FESEM) with energy dispersive spectroscopy (EDS). It has been observed that hardness is increased by increasing the sintering temperature in the case of SiC, whereas surface roughness did not change much by changing the composition. Additionally, a rise in temperature lead to liquid-state sintering. SEM images obtained during the elemental analysis showed that porosity is generated within the samples after sintering due to the higher melting point of reinforcements compared to a base metal, i.e., aluminum. Mathematical equations have also been developed via regression analysis using Minitab and excel for the confirmation and validation of experimental data. Analysis of Variance (ANOVA) has also been done, and its tables are shown and discussed in the paper. Hence, the most optimized findings relating the changes in the composition of reinforcements, sintering temperature, and sintering time (input variables) with porosity, hardness, and surface roughness have been presented in the current study.
Notes on the Treatment of Charged Particles for Studying Cyclotide/Membrane Interactions with Dissipative Particle Dynamics
Authors: Bänsch Felix , Steinbeck Christoph , Zielesny Achim
Published: June 2022

Different charge treatment approaches are examined for cyclotide-induced plasma membrane disruption by lipid extraction studied with dissipative particle dynamics. A pure Coulomb approach with truncated forces tuned to avoid individual strong ion pairing still reveals hidden statistical pairing effects that may lead to artificial membrane stabilization or distortion of cyclotide activity depending on the cyclotide’s charge state. While qualitative behavior is not affected in an apparent manner, more sensitive quantitative evaluations can be systematically biased. The findings suggest a charge smearing of point charges by an adequate charge distribution. For large mesoscopic simulation boxes, approximations for the Ewald sum to account for mirror charges due to periodic boundary conditions are of negligible influence.
Research in the Field of Exercise and Metabolomics: A Bibliometric and Visual Analysis
Authors: Lv Zhen , Gong Zhi-Gang , Xu Yong-Jiang
Published: June 2022

The aim of this article was to conduct a bibliometric analysis of global research trends in the field of exercise and metabolomics between 2005 and 2020. Systematic articles were obtained from the literature in the Web of Science core collection database from 2005 to 2020. The relationship between the number of publications, citations, countries, journals, authors, and the evolution of research hotspots was analyzed. A total of 807 studies were included in the analysis. From 2005 to 2020, the number of citations and the number of published articles showed an upward trend. Keyword co-occurrence indicates that research hotspots are focused on exercise, physical activity, metabolomics, obesity, insulin resistance, inflammation, and cardiovascular disease. Keyword clustering indicates that the research frontier is focused on the field of sports medicine, which includes molecular-level studies of exercise interventions in disease and studies of the physiological mechanisms by which exercise alters the body. Overall, this trinity of models, combining chronic disease with exercise interventions and molecular-level studies of metabolomics, has become the forefront of research in the field. This historical review of the field of exercise and metabolomics will further provide a useful basis for hot issues and future development trends.
Robust Touch Screen Readout System to Display Noise Using Multireference Differential Sensing Scheme for Flexible AMOLED Display
Authors: Lee Junmin , Kim Hyoyoung , Ham Juwon , Ko Seunghoon
Published: June 2022

This paper presents a front-end architecture for touch screen panel (TSP) readout in a TSP-integrated, ultrathin flexible display to mitigate severe display noise interference, which is an uncommon mode caused by the large panel load of the TSP in the flexible display. The differential sensing method with multireference TSP channels minimized an imbalance of the phase and amplitude of the coupled-display noise interference. In addition, cascaded time-discrete bandpass sampling was employed to enhance the touch sensitivity in the sensing block. Moreover, a rated front-end block could be reconfigured to a differential or single-ended sensing structure, which reused the prefilter capacitors in the differential sensing for offset cancellation in reference capacitance sensing. To further improve the sensitivity, programmable postfiltering was employed on the reference TSP channels. Subsequently, the proposed front-end was implemented in a 350 nm process, wherein it achieved a SNR of 50.5 dB with a scan rate of 200 Hz and attenuated aggravated display noise interference by more than 6.84 dB as compared to the conventional differential sensing method. The designed chip occupied an area of 4.8 mm2 and consumed 17.6 mW from a 3 V supply.