bluuebunny/crossref_metadata_2025 huggingface dataset:
Responses of the Necrotrophic Fungus Alternaria brassisicola to the Indolic Phytoalexin Brassinin
Authors: N’Guyen Guillaume Quang , Raulo Roxane , Porquier Antoine , Iacomi Beatrice , Pelletier Sandra , Renou Jean-Pierre , Bataillé-Simoneau Nelly , Campion Claire , Hamon Bruno , Kwasiborski Anthony , Colou Justine , Benamar Abdelilah , Hudhomme Pietrick , Macherel David , Simoneau Philippe , Guillemette Thomas
Published: January 2021

Alternaria brassicicola causes black spot disease in Brassicaceae. During host infection, this necrotrophic fungus is exposed to various antimicrobial compounds, such as the phytoalexin brassinin which is produced by many cultivated Brassica species. To investigate the cellular mechanisms by which this compound causes toxicity and the corresponding fungal adaptive strategies, we first analyzed fungal transcriptional responses to short-term exposure to brassinin and then used additional functional approaches. This study supports the hypothesis that indolic phytoalexin primarily targets mitochondrial functions in fungal cells. Indeed, we notably observed that phytoalexin treatment of A. brassicicola disrupted the mitochondrial membrane potential and resulted in a significant and rapid decrease in the oxygen consumption rates. Secondary effects, such as Reactive oxygen species production, changes in lipid and endoplasmic reticulum homeostasis were then found to be induced. Consequently, the fungus has to adapt its metabolism to protect itself against the toxic effects of these molecules, especially via the activation of high osmolarity glycerol and cell wall integrity signaling pathways and by induction of the unfolded protein response.
Transcription Factor VviMYB86 Oppositely Regulates Proanthocyanidin and Anthocyanin Biosynthesis in Grape Berries
Authors: Cheng Jing , Yu Keji , Shi Ying , Wang Jun , Duan Changqing
Published: January 2021

Proanthocyanidins (PAs) and anthocyanins are two vital groups of flavonoid compounds for grape berries and red wines. Several transcription factors (TFs) have been identified to be involved in regulating PA and anthocyanin biosynthesis in grape berries. However, research on TFs with different regulatory mechanisms for these two biosynthesis branches in grapes remains limited. In this study, we identified an R2R3-MYB TF, VviMYB86, whose spatiotemporal gene expression pattern in grape berries coincided well with PA accumulation but contrasted with anthocyanin synthesis. Bothin vivoandin vitroexperiments verified that VviMYB86 positively regulated PA biosynthesis, primarily by upregulating the expression of the two leucoanthocyanidin reductase (LAR) genes in the Arabidopsis protoplast system, as well as inVviMYB86-overexpressing grape callus cultured under 24 h of darkness. Moreover, VviMYB86 was observed to repress the anthocyanin biosynthesis branch in grapes by downregulating the transcript levels ofVviANSandVviUFGT. Overall, VviMYB86 is indicated to have a broad effect on flavonoid synthesis in grape berries. The results of this study will help elucidate the regulatory mechanism governing the expression of the two LAR genes in grape berries and provide new insights into the regulation of PA and anthocyanin biosynthesis in grape berries.
Interspecific Variation in the Unsaturation Level of Seed Oils Were Associated With the Expression Pattern Shifts of Duplicated Desaturase Genes and the Potential Role of Other Regulatory Genes
Authors: Wang Mengli , Gao Lexuan , Li Gengyun , Zhou Chengchuan , Jian Jinjing , Xing Zhen , Wang Yuguo , Zhang Wenju , Song Zhiping , Hu Yonghong , Yang Ji
Published: January 2021

Seed oils are of great economic importance both for human consumption and industrial applications. The nutritional quality and industrial value of seed oils are mostly determined by their fatty acid profiles, especially the relative proportions of unsaturated fatty acids. Tree peony seed oils have recently been recognized as novel edible oils enriched in α-linolenic acid (ALA). However, congeneric species, such as Paeonia ostii and P. ludlowii, showed marked variation in the relative proportions of different unsaturated fatty acids. By comparing the dynamics of fatty acid accumulation and the time-course gene expression patterns between P. ostii and P. ludlowii, we identified genes that were differentially expressed between two species in developing seeds, and showed congruent patterns of variation between expression levels and phenotypes. In addition to the well-known desaturase and acyltransferase genes associated with fatty acid desaturation, among them were some genes that were conservatively co-expressed with the desaturation pathway genes across phylogenetically distant ALA-rich species, including Camelina sativa and Perilla frutescens. Go enrichment analysis revealed that these genes were mainly involved in transcriptional regulation, protein post-translational modification and hormone biosynthesis and response, suggesting that the fatty acid synthesis and desaturation pathway might be subject to multiple levels of regulation.
Expectancy to Eat Modulates Cognitive Control and Attention Toward Irrelevant Food and Non-food Images in Healthy Starving Individuals. A Behavioral Study
Authors: Schiff Sami , Testa Giulia , Rusconi Maria Luisa , Angeli Paolo , Mapelli Daniela
Published: January 2021

It is thought that just as hunger itself, the expectancy to eat impacts attention and cognitive control toward food stimuli, but this theory has not been extensively explored at a behavioral level. In order to study the effect of expectancy to eat on attentional and cognitive control mechanisms, 63 healthy fasting participants were presented with an affective priming spatial compatibility Simon task that included both food and object (non-food) distracters. The participants (N = 63) were randomly assigned to two groups: an “immediate expectancy” group made up of participants who expected to eat immediately after the task (N = 31; females = 21; age = 26.8 ± 9.6) and a “delayed expectancy” cohort made up of individuals who expected to eat a few hours later (N = 32; females = 21; age = 25.0 ± 8.0). Slower reaction times (RTs) toward the food and non-food distracters and a more pronounced effect on the RTs in the incompatible condition [i.e., the Simon effect (SE)] were noted in both groups. The effect of the food and non-food distracters on the RTs was more pronounced in the immediate with respect to the delayed expectancy group. The magnitude of the SE for the food and the non-food distracters was also greater in the immediate with respect to the delayed expectancy group. These results seem to indicate that when the expectancy to eat is short, the RTs are delayed, and the SE is more pronounced when food and non-food distracters are presented. Instead, when the expectancy to eat is more distant, the distracters have less of an effect on the RTs and the correspondence effect is smaller. Our results suggest that the expectancy to eat can modulate both attention orienting and cognitive control mechanisms in healthy fasting individuals when distracting details are competing with information processing during goal directed behavior.
Cardiac and Proprioceptive Accuracy Are Not Related to Body Awareness, Perceived Body Competence, and Affect
Authors: Horváth Áron , Vig Luca , Ferentzi Eszter , Köteles Ferenc
Published: January 2021

Interoception in the broader sense refers to the perception of internal states, including the perception of the actual state of the internal organs (visceroception) and the motor system (proprioception). Dimensions of interoception include (1) interoceptive accuracy, i.e., the ability to sense internal changes assessed with behavioral tests, (2) confidence rating with respect to perceived performance in an actual behavioral test, and (3) interoceptive sensibility, i.e., the self-reported generalized ability to perceive body changes. The relationship between dimension of cardioceptive and proprioceptive modalities and their association with affect are scarcely studied. In the present study, undergraduate students (N = 105, 53 males, age: 21.0 ± 1.87 years) filled out questionnaires assessing positive and negative affect (Positive and Negative Affect Schedule), interoceptive sensibility (Body Awareness Questionnaire), and body competence (Body Competence Scale of the Body Consciousness Questionnaire). Following this, they completed a behavioral task assessing cardioceptive accuracy (the mental heartbeat tracking task by Schandry) and two tasks assessing proprioceptive accuracy with respect to the tension of arm flexor muscles (weight discrimination task) and the angular position of the elbow joint (joint position reproduction task). Confidence ratings were measured with visual analog scales after the tasks. With the exception of a weak association between cardioceptive accuracy and the respective confidence rating, no associations between and within modalities were found with respect to various dimensions of interoception. Further, the interoceptive dimensions were not associated with state and trait positive and negative affect and perceived body competence. In summary, interoceptive accuracy scores do not substantially contribute to conscious representations of cardioceptive and proprioceptive ability. Within our data, non-pathological affective states (PANAS) are not associated with the major dimensions of interoception for the cardiac and proprioceptive modalities.
Mental Health Outcomes Amongst Health Care Workers During COVID 19 Pandemic in Saudi Arabia
Authors: Al Ammari Maha , Sultana Khizra , Thomas Abin , Al Swaidan Lolowa , Al Harthi Nouf
Published: January 2021

Objectives: The study aimed to assess the mental health outcomes and associated factors among health care workers during COVID 19 in Saudi Arabia.

Design, Setting, and Participants: We conducted a cross-sectional survey of health care workers from tertiary care and ministry of health Centers across the Central, Eastern, and Western regions of Saudi Arabia. There were 1,130 participants in the survey, and we collected demographic and mental health measurements from the participants.

Primary Outcomes and Measures: The magnitude of symptoms of depression, anxiety, and insomnia was measured using the original version of 9-item patient health questionnaire (PHQ-9), the 7-item generalized anxiety disorder scale (GAD-7), and 7-item insomnia severity index (ISI). We use the multiple logistic regression analysis to identify the associated risk factors of individual outcomes.

Results: The scores on the PHQ-9 showed that the largest proportion of health care workers (76.93%) experienced only normal to mild depression (50.83 and 26.1%, respectively). The scores on the GAD-7 showed that the largest proportion of health care workers (78.88%) experienced minimal to mild anxiety (50.41 and 28.47%, respectively). The scores on the ISI showed that the largest proportion of health care workers (85.83%) experienced absence to subthreshold insomnia (57.08 and 28.75%, respectively). The risk factors for depression in health care workers were Saudi, living with family, working from an isolated room at home and frontline worker. For anxiety, being female was risk factor and for insomnia, being frontline worker was risk factor.

Conclusion: It was observed that the symptoms of depression, anxiety, and insomnia were reported in a lower proportion of health care workers in our study. The participants who were female, frontline workers, Saudi, living with family, and working from home in isolated rooms were predisposed to developing psychological disorders.
Mathematical Modeling Predicts That Strict Social Distancing Measures Would Be Needed to Shorten the Duration of Waves of COVID-19 Infections in Vietnam
Authors: Bouchnita Anass , Chekroun Abdennasser , Jebrane Aissam
Published: January 2021

Coronavirus disease 2019 (COVID-19) emerged in Wuhan, China in 2019, has spread throughout the world and has since then been declared a pandemic. As a result, COVID-19 has caused a major threat to global public health. In this paper, we use mathematical modeling to analyze the reported data of COVID-19 cases in Vietnam and study the impact of non-pharmaceutical interventions. To achieve this, two models are used to describe the transmission dynamics of COVID-19. The first model belongs to the susceptible-exposed-infectious-recovered (SEIR) type and is used to compute the basic reproduction number. The second model adopts a multi-scale approach which explicitly integrates the movement of each individual. Numerical simulations are conducted to quantify the effects of social distancing measures on the spread of COVID-19 in urban areas of Vietnam. Both models show that the adoption of relaxed social distancing measures reduces the number of infected cases but does not shorten the duration of the epidemic waves. Whereas, more strict measures would lead to the containment of each epidemic wave in one and a half months.
Phosphorus Nutrition and Growth of Cotton Plants Inoculated With Growth-Promoting Bacteria Under Low Phosphate Availability
Authors: Romero-Perdomo Felipe , Beltrán Isidro , Mendoza-Labrador Jonathan , Estrada-Bonilla German , Bonilla Ruth
Published: January 2021

The low availability of phosphorus (P) in the soil drastically limits the world productivity of crops such as cotton. In order to contribute sustainably to the solution of this problem, the current study aimed to evaluate the capacity of phosphate-solubilising bacteria to improve plant growth and its relationship with physiological parameters, as well as the shoot P content in cotton plants in a soil with low P availability amended with rock phosphate. The results showed that, of the six plant growth-promoting bacteria strains evaluated under greenhouse conditions, the Rhizobium strain B02 significantly promoted growth, shoot P content and photosynthetic rate. This strain also improved the transpiration rate and the relative content of chlorophyll but without significant differences. Remarkably, Rhizobium sp. B02 had a more significant effect on plant growth compared to the P nutrition. Furthermore, the effect of its inoculation was more pronounced on the roots' growth compared to the shoot. Finally, application of Rhizobium strain B02 showed the capacity to optimize the use of low-solubility fertilizer as the rock phosphate. These findings could be associated with the metabolic activities of plant growth promotion exhibited by phosphate-solubilising strains, such as phosphate solubilisation, production of indole compounds and siderophores synthesis. In conclusion, this research provides evidence of the biotechnological potential of the Rhizobium genus as phosphate-solubilising bacteria with multiple plant growth-promoting activities capable of improving the plant growth and phosphate nutrition of non-leguminous crops such as cotton in soil with low P availability amended with rock phosphate.
Decomposition-Based Soil Moisture Estimation Using UAVSAR Fully Polarimetric Images
Authors: Akhavan Zeinab , Hasanlou Mahdi , Hosseini Mehdi , McNairn Heather
Published: January 2021

Polarimetric decomposition extracts scattering features that are indicative of the physical characteristics of the target. In this study, three polarimetric decomposition methods were tested for soil moisture estimation over agricultural fields using machine learning algorithms. Features extracted from model-based Freeman–Durden, Eigenvalue and Eigenvector based H/A/α, and Van Zyl decompositions were used as inputs in random forest and neural network regression algorithms. These algorithms were applied to retrieve soil moisture over soybean, wheat, and corn fields. A time series of polarimetric Uninhabited Aerial Vehicle Synthetic Aperture Radar (UAVSAR) data acquired during the Soil Moisture Active Passive Experiment 2012 (SMAPVEX12) field campaign was used for the training and validation of the algorithms. Three feature selection methods were tested to determine the best input features for the machine learning algorithms. The most accurate soil moisture estimates were derived from the random forest regression algorithm for soybeans, with a correlation of determination (R2) of 0.86, root mean square error (RMSE) of 0.041 m3 m−3 and mean absolute error (MAE) of 0.030 m3 m−3. Feature selection also impacted results. Some features like anisotropy, Horizontal transmit and Horizontal receive (HH), and surface roughness parameters (correlation length and RMS-H) had a direct effect on all algorithm performance enhancement as these parameters have a direct impact on the backscattered signal.
Wild and Cultivated Homoeologous Barley Chromosomes Can Associate and Recombine in Wheat in the Absence of the Ph1 Locus
Authors: Calderón María Carmen , Prieto Pilar
Published: January 2021

Bread wheat is an allohexaploid that behaves as a diploid during meiosis, the cell division process to produce the gametes occurring in organisms with sexual reproduction. Knowledge of the mechanisms implicated in meiosis can contribute to facilitating the transfer of desirable traits from related species into a crop like wheat in the framework of breeding. It is particularly interesting to shed light on the mechanisms controlling correct pairing between homologous (equivalent) chromosomes and recombination, even more in polyploid species. The Ph1 (Pairing homoeologous 1) locus is implicated in recombination. In this work, we aimed to study whether homoeologous (equivalent chromosomes from different genomes) Hordeum chilense (wild barley) and H. vulgare (cultivated barley) chromosomes can associate and recombine during meiosis in the wheat background in the absence of the Ph1 locus. For this, we have developed H. chilense and H. vulgare double monosomic addition lines for the same and for different homoeology group in wheat in the ph1b mutant background. Using genomic in situ hybridization, we visualized the two (wild and cultivated) barley chromosomes during meiosis and we studied the processes of recognition, association, and recombination between homoeologous chromosomes in the absence of the Ph1 locus. Our results showed that the Ph1 locus does not prevent homoeologous chromosome pairing but it can regulate recombination.
Systematic Analysis of the bZIP Family in Tobacco and Functional Characterization of NtbZIP62 Involvement in Salt Stress
Authors: Li Zhiyuan , Chao Jiangtao , Li Xiaoxu , Li Gongbo , Song Dean , Guo Yongfeng , Wu Xinru , Liu Guanshan
Published: January 2021

The basic leucine zipper (bZIP) transcription factors play important regulatory roles, influencing plant growth and responses to environmental stresses. In the present study, 132 bZIP genes identified in the tobacco genome were classified into 11 groups with Arabidopsis and tomato bZIP members, based on the results of a phylogenetic analysis. An examination of gene structures and conserved motifs revealed relatively conserved exon/intron structures and motif organization within each group. The results of an investigation of whole-genome duplication events indicated that segmental duplications were crucial for the expansion of the bZIP gene family in tobacco. Expression profiles confirmed that the NtbZIP genes are differentially expressed in various tissues, and several genes are responsive to diverse stresses. Notably, NtbZIP62, which was identified as an AtbZIP37/ABF3 homolog, was highly expressed in response to salinity. Subcellular localization analyses proved that NtbZIP62 is a nuclear protein. Furthermore, the overexpression of NtbZIP62 in tobacco significantly enhanced the salt stress tolerance of the transgenic plants. The results of this study may be relevant for future functional analyses of the bZIP genes in tobacco.
Predicting Net Returns of Organic and Conventional Strawberry Following Soil Disinfestation with Steam or Steam Plus Additives
Authors: Michuda Aleksandr , Goodhue Rachael E. , Hoffmann Mark , Fennimore Steven A.
Published: January 2021

Pre-plant methods for managing soil-borne pests and diseases are an important priority for many agricultural production systems. This study investigates whether the application of steam is an economically sustainable pre-plant soil disinfestation technique for organic and conventional strawberry (Fragaria ananassa) production in California’s Central Coast region. We analyze net returns from field trials using steam and steam + mustard seed meal (MSM) as pre-plant soil disinfestation treatments. ANOVA tests identify statistically significant differences in net revenues by treatment and trial. Multivariate regressions estimate the magnitude of these effects. Predictive polynomial models identify relationships between net returns and two treatment characteristics: maximum temperature (°C) and time at ≥60 °C (minutes). For organic production, net returns are statistically similar for the steam and steam + MSM treatments. For conventional production, the steam + MSM treatment has significantly higher net returns than the steam treatment. Cross-validated polynomial models outperform the sample mean for prediction of net returns, except for the steam + MSM treatment in conventional production. The optimal degree of the polynomial ranges from 1–4 degrees, depending on the production system and treatment. Results from two of three organic models suggest that maximum soil temperatures of 62–63 °C achieved for 41–44 min maximizes net returns and may be a basis for further experiments.
Impact of Genotype, Weather Conditions and Production Technology on the Quantitative Profile of Anti-Nutritive Compounds in Rye Grains
Authors: Grabiński Jerzy , Sułek Alicja , Wyzińska Marta , Stuper-Szablewska Kinga , Cacak-Pietrzak Grażyna , Nieróbca Anna , Dziki Dariusz
Published: January 2021

The main anti-nutritive substances present in rye grains include alkylresorcinols, water-soluble pentosans, and trypsin inhibitors. The content of these compounds in the grains can be influenced by genetic factors, habitat conditions and the crop management technology used in cultivation. The aim of the study was to determine the variability of the concentration of anti-nutritive compounds in rye grains depending on the variety, weather conditions and production technology. The field research was conducted at the IUNG-PIB Experimental Station in Osiny (Poland) in three growing seasons 2010/2011, 2011/2012 and 2012/2013. The experiment was located on lessive soil, on plots of 45 m2 in three replications. The first factor was the type of production technology (integrated and intensive), while the second was a variety of winter rye. Two hybrid varieties (Brasetto, Visello) and two population varieties (Dańkowskie Diament, Kier) were included. The research showed that the content of anti-nutritive compounds in rye grains depended significantly on the genotype and weather conditions. Stress conditions during the rye growing season in 2011 and 2013 were the cause of higher synthesis of alkylresorcinols, soluble pentosans and trypsin inhibitors in rye grains. The content of alkylresorcinols and water-soluble pentosans also depended significantly on the intensity of the production technology. The higher content of these compounds was found in rye grains from intensive technology. The production technology had no significant impact on the activity of trypsin inhibitors.
Effect of Attractants and Micronutrient Biofortification on the Yield and Quality of Red Clover (Trifolium pratense L.) Seeds
Authors: Przybylska Anna , Ćwintal Marek , Pszczółkowski Piotr , Sawicka Barbara
Published: January 2021

The aim of the research was to develop an innovative technique in the seed production of red clover. The research was carried out in 2013–2016, in Felin (51°22′ N, 22°65′ E), in a dependent arrangement (split-split-plot) in four replications. The first order factor was the use of an attractant: (1) control object, with pure water spraying, (2) attractant used in the 60 BBCH phase, (3) attractant applied twice—in the 60 and 65 phase, in the BBCH scale. The factor of the second order was fertilization: (a) standard object with PK fertilization; (b) fertilization with microelements (B + Mo) against the background of constant PK fertilization. The use of attractant ‘Pollinus’ preparation in the cultivation of red clover, both once and twice, in the form of spraying on plants, contributed to an increase in the seed yield, number of heads, share of seeds in the head, thousand seeds weight, and seed germination capacity, compared to the control object. Elements of the red clover yield structure achieved more favorable values with foliar feeding (B + Mo) compared to the standard one. Under the influence of plant biofortification with boron and molybdenum, the number of seeds infected with fungal pathogens and abnormally germinating decreased.
Luteinizing Hormone Effect on Luteal Cells Is Dependent on the Corpus Luteum Stage in Felids
Authors: Hryciuk Michał M. , Jewgenow Katarina , Braun Beate C.
Published: January 2021

The objective of this study was to investigate the effect of luteinizing hormone (LH) on steroidogenic luteal cells obtained from corpora lutea (CL) of the domestic cat and selected wild felids. Luteal cells were isolated enzymatically from CL at different developmental stages and cultured for two days in the presence and absence of 100 ng/mL LH, respectively. Functionality was assessed by progesterone (P4) accumulation in cell culture media determined by ELISA. In addition, steroidogenic function was confirmed using immunohistochemistry for 3β-hydroxysteroid dehydrogenase (HSD3B). The enzymatic method allowed for the isolation of mostly small luteal cells in all investigated felids. Treatment with LH resulted in an increase in P4 secretion of cultured luteal cells obtained from CL in the formation stage (African lion) and development/maintenance stage (domestic cat (p < 0.05), Javan leopard), whereas luteal cells from more advanced stages of luteal development (regression) responded moderately or not at all to LH stimulation (domestic cat, Asiatic golden cat, Asiatic lion). The protein signal for HSD3B on CL was visible until development/maintenance. In conclusion, this study shows that LH promotes P4 production in luteal cells only until the onset of regression, when morphological signs are visible on the CL of felids and HSD3B is no longer detectable.
Antibody Identification for Antigen Detection in Formalin-Fixed Paraffin-Embedded Tissue Using Phage Display and Naïve Libraries
Authors: Mairaville Célestine , Martineau Pierre
Published: January 2021

Immunohistochemistry is a widely used technique for research and diagnostic purposes that relies on the recognition by antibodies of antigens expressed in tissues. However, tissue processing and particularly formalin fixation affect the conformation of these antigens through the formation of methylene bridges. Although antigen retrieval techniques can partially restore antigen immunoreactivity, it is difficult to identify antibodies that can recognize their target especially in formalin-fixed paraffin-embedded tissues. Most of the antibodies currently used in immunohistochemistry have been obtained by animal immunization; however, in vitro display techniques represent alternative strategies that have not been fully explored yet. This review provides an overview of phage display-based antibody selections using naïve antibody libraries on various supports (fixed cells, dissociated tissues, tissue fragments, and tissue sections) that have led to the identification of antibodies suitable for immunohistochemistry.
A Molecule of the Viridomycin Family Originating from a Streptomyces griseus-Related Strain Has the Ability to Solubilize Rock Phosphate and to Inhibit Microbial Growth
Authors: Hamdali Hanane , Lebrihi Ahmed , Monje Marie Carmen , Benharref Ahmed , Hafidi Mohamed , Ouhdouch Yedir , Virolle Marie Joëlle
Published: January 2021

Some soil-borne microorganisms are known to have the ability to solubilize insoluble rock phosphate and this process often involves the excretion of organic acids. In this issue, we describe the characterization of a novel solubilizing mechanism used by a Streptomyces strain related to Streptomyces griseus isolated from Moroccan phosphate mines. This process involves the excretion of a compound belonging to the viridomycin family that was shown to play a major role in the rock phosphate bio weathering process. We propose that the chelation of the positively charged counter ions of phosphate constitutive of rock phosphate by this molecule leads to the destabilization of the structure of rock phosphate. This would result in the solubilization of the negatively charged phosphates, making them available for plant nutrition. Furthermore, this compound was shown to inhibit growth of fungi and Gram positive bacteria, and this antibiotic activity might be due to its strong ability to chelate iron, a metallic ion indispensable for microbial growth. Considering its interesting properties, this metabolite or strains producing it could contribute to the development of sustainable agriculture acting as a novel type of slow release bio-phosphate fertilizer that has also the interesting ability to limit the growth of some common plant pathogens.
Potential Antioxidant Properties of Enzymatic Hydrolysates from Stichopus japonicus against Hydrogen Peroxide-Induced Oxidative Stress
Authors: Lee Hyo-Geun , Kim Hyun-Soo , Oh Jae-Young , Lee Dae-Sung , Yang Hye-Won , Kang Min-Cheol , Kim Eun-A , Kang Nalae , Kim Junseong , Heo Soo-Jin , Jeon You-Jin
Published: January 2021

A comprehensive antioxidant evaluation was performed on enzymatic hydrolysates of Stichopusjaponicus (S. japonicus) using Vero cells and zebrafish models for in vitro and in vivo studies, respectively. S. japonicus was hydrolyzed with food-grade enzymes (alcalase, α-chymotrypsin, flavourzyme, kojizyme, neutrase, papain, pepsin, protamex, and trypsin), and the free radical scavenging activities were screened via electron spin resonance (ESR) spectroscopy. According to the results, the enzymatic hydrolysates contained high protein and relatively low polysaccharide and sulfate contents. Among these hydrolysates, the α-chymotrypsin assisted hydrolysate from S. japonicus (α-chy) showed high yield and protein content, and strong hydroxyl radical scavenging activity. Therefore, α-chy was chosen for further purification. The α-chy was fractionated by ultrafiltration into three ultrafiltration (UF) fractions based on their molecular weight: >10 kDa (α-chy-I), 5–10 kDa (α-chy-II), and <5 kDa (α-chy-III), and we evaluated their antioxidant properties in H2O2 exposed Vero cells. The α-chy and its UF fractions significantly decreased the intracellular reactive oxygen species (ROS) generation and increased cell viability in H2O2 exposed Vero cells. Among them, α-chy-III effectively declined the intracellular ROS levels and increased cell viability and exhibited protection against H2O2 induced apoptotic damage. Furthermore, α-chy-III remarkably attenuated the cell death, intracellular ROS and lipid peroxidation in H2O2 exposed zebrafish embryos. Altogether, our findings demonstrated that α-chy and its α-chy-III from S. japonicus possess strong antioxidant activities that could be utilized as a bioactive ingredient for functional food industries.
Effects of Simultaneous Cognitive Task on Gait Event Accuracy with Auditory Stimuli: Comparison between Young Adults in Their 20s and the Elderly in their 70s
Authors: Choi Jin-Seung , Kim Jung-Gil , Cho Jun-Hyeong , Tack Gye-Rae
Published: January 2021

The purpose of this study was to compare the difference in the accuracy of gait events between young and older adults during metronomic walking by auditory cueing. Additionally, age-specific changes in the gait event accuracy according to additional simultaneous cognitive tasks were examined. The time interval (or temporal error) between the auditory cue (i.e., metronome) and the heel contact was used as the accuracy of the gait event. Fifteen young group (YG, 24.7 ± 0.8 years) and 14 elderly (EG, 78.4 ± 5.5 years) people participated in the experiment. The temporal errors under two gait conditions (MET: walking with metronome; MET + BC: walking with metronome while counting backward) were compared for each group. The results revealed that all the temporal errors of EG were significantly greater than those of YG. While the addition of simultaneous cognitive tasks resulted in a significant increase in temporal error in both age groups, the coefficient of variation (CV) of the temporal error significantly increased only in the EG group. In other words, although heel contact accuracy with auditory stimuli was affected by the simultaneous cognitive task in both groups, it was demonstrated that the variability of the error in the young adults remained constant. Therefore, the time error measurement used in this study has the potential to be used as a tool to judge the gait instability of the elderly compared with young adults.
Functions and Therapeutic Potential of Extracellular Hsp60, Hsp70, and Hsp90 in Neuroinflammatory Disorders
Authors: Alberti Giusi , Paladino Letizia , Vitale Alessandra Maria , Caruso Bavisotto Celeste , Conway de Macario Everly , Campanella Claudia , Macario Alberto J. L. , Marino Gammazza Antonella
Published: January 2021

Neuroinflammation is implicated in central nervous system (CNS) diseases, but the molecular mechanisms involved are poorly understood. Progress may be accelerated by developing a comprehensive view of the pathogenesis of CNS disorders, including the immune and the chaperone systems (IS and CS). The latter consists of the molecular chaperones; cochaperones; and chaperone cofactors, interactors, and receptors of an organism and its main collaborators in maintaining protein homeostasis (canonical function) are the ubiquitin–proteasome system and chaperone-mediated autophagy. The CS has also noncanonical functions, for instance, modulation of the IS with induction of proinflammatory cytokines. This deserves investigation because it may be at the core of neuroinflammation, and elucidation of its mechanism will open roads toward developing efficacious treatments centered on molecular chaperones (i.e., chaperonotherapy). Here, we discuss information available on the role of three members of the CS—heat shock protein (Hsp)60, Hsp70, and Hsp90—in IS modulation and neuroinflammation. These three chaperones occur intra- and extracellularly, with the latter being the most likely involved in neuroinflammation because they can interact with the IS. We discuss some of the interactions, their consequences, and the molecules involved but many aspects are still incompletely elucidated, and we hope that this review will encourage research based on the data presented to pave the way for the development of chaperonotherapy. This may consist of blocking a chaperone that promotes destructive neuroinflammation or replacing or boosting a defective chaperone with cytoprotective activity against neurodegeneration.
Monitoring the Antibacterial Effect of Rosin Acids in an Austrian Beet Sugar Plant by Amplicon-Based Sequencing and Flow Cytometry
Authors: Moser Cordula K. , Ukowitz Christina , Zitz Ulrike , Emerstorfer Florian , Hein Walter , Kneifel Wolfgang , Domig Konrad J.
Published: January 2021

For decades, microorganisms in beet sugar production have been studied using culture-based methods. However, these methods are not sufficient to describe such a complex bacterial community. In this study, therefore, an amplicon-based sequencing technique (Illumina MiSeq platform) was applied to characterize the bacterial community and its dynamics in the extraction area and juice purification station of an Austrian beet sugar plant. Depending on the process conditions thermophilic bacteria, such as Geobacillus spp., Caenibacillus spp., and Thermus spp., and mesophilic bacteria, such as Leuconostoc spp. and Bacillus spp., were found. Besides these microbiological characteristics, the antimicrobial effect of a rosin acid-based product (Defostab 220) on the bacterial communities was investigated in industrial and laboratory trials. The antimicrobial effect of a given concentration of rosin acid varies from bacteriostatic to bactericidal effects on different occurring groups of bacteria.
Comparison of Hamstrings and Quadriceps Muscle Activation in Male and Female Professional Soccer Players
Authors: Torres Gonzalo , Armada-Cortés Estrella , Rueda Javier , San Juan Alejandro F. , Navarro Enrique
Published: January 2021

(1) Background: this study aimed to determine if there are differences in quadriceps and hamstring muscle activation in professional male and female soccer players. (2) Methods: muscle activation was recorded by surface electromyography in 27 professional soccer players (19 male and 8 female). The players performed the Bulgarian squat and lunge exercises. Vastus medialis, vastus lateralis, rectus femoris, semitendinosus, and biceps femoris were the muscles analyzed. (3) Results: The statistical analysis of the hamstring:quadriceps ratio showed no significant differences (p > 0.05). Significant differences were found in the vastus medialis:vastus lateralis ratio for both the lunge exercise (t20 = 3.35; p = 0.001; d = 1.42) and the Bulgarian squat (t23 = 4.15; p < 0.001; d = 1.76). For the intragroup muscular pattern in the lunge and Bulgarian squat exercises, the female players showed higher activation for the vastus lateralis muscle (p < 0.001) than the male players and lower muscle activation in the vastus medialis. No significant differences were found in the rectus femoris, biceps remoris, and semitendinosus muscles (p > 0.05). (4) Conclusions: Differences were found in the medial ratio (vastus medialis: vastus lateralis). Moreover, regarding the intramuscular pattern, very consistent patterns have been found. In the quadriceps muscle: VM>VL>RF; in the hamstring muscle: ST>BF. These patterns could be very useful in the recovery process from an injury to return players to their highest performance.
Classification and Quantification of Human Error in Manufacturing: A Case Study in Complex Manual Assembly
Authors: Torres Yaniel , Nadeau Sylvie , Landau Kurt
Published: January 2021

Manual assembly operations are sensitive to human errors that can diminish the quality of final products. The paper shows an application of human reliability analysis in a realistic manufacturing context to identify where and why manual assembly errors occur. The techniques SHERPA and HEART were used to perform the analysis of human reliability. Three critical tasks were selected for analysis based on quality records: (1) installation of three types of brackets using fasteners, (2) fixation of a data cable to the assembly structure using cushioned loop clamps and (3) installation of cap covers to protect inlets. The identified error modes with SHERPA were: 36 action errors, nine selection errors, eight information retrieval errors and six checking errors. According to HEART, the highest human error probabilities were associated with assembly parts sensitive to geometry-related errors (brackets and cushioned loop clamps). The study showed that perceptually engaging assembly instructions seem to offer the highest potential for error reduction and performance improvement. Other identified areas of action were the improvement of the inspection process and workers’ provision with better tracking and better feedback. Implementation of assembly guidance systems could potentially benefit worker’s performance and decrease assembly errors.
Recycling Biogas Digestate from Energy Crops: Effects on Soil Properties and Crop Productivity
Authors: Pastorelli Roberta , Valboa Giuseppe , Lagomarsino Alessandra , Fabiani Arturo , Simoncini Stefania , Zaghi Massimo , Vignozzi Nadia
Published: January 2021

Digestate from biogas production can be recycled to the soil as conditioner/fertilizer improving the environmental sustainability of the energy supply chain. In a three-year maize-triticale rotation, we investigated the short-term effects of digestate on soil physical, chemical, and microbiological properties and evaluated its effectiveness in complementing the mineral fertilizers. Digestate soil treatments consisted of combined applications of the whole digestate and its mechanically separated solid fraction. Digestate increased soil total organic C, total N and K contents. Soil bulk density was not affected by treatments, while aggregate stability showed a transient improvement due to digestate treatments. A decrement of the transmission pores proportion and an increment of fissures was observed in digestate treated soils. Soil microbial community was only transiently affected by digestate treatments and no soil contamination from Clostridiaceae-related bacteria were observed. Digestate can significantly impair seed germination when applied at low dilution ratios. Crop yield under digestate treatment was similar to ordinary mineral-based fertilization. Overall, our experiment proved that the agronomic recycling of digestate from biogas production maintained a fair crop yield and soil quality. Digestate was confirmed as a valid resource for sustainable management of soil fertility under energy-crop farming, by combining a good attitude as a fertilizer with the ability to compensate for soil organic C loss.
Knud Rasmussen Glacier Status Analysis Based on Historical Data and Moving Detection Using RPAS
Authors: Pavelka Karel , Šedina Jaroslav , Pavelka Karel
Published: January 2021

This article discusses partial results of an international scientific expedition to Greenland that researched the geography, geodesy, botany, and glaciology of the area. The results here focus on the photogrammetrical results obtained with the eBee drone in the eastern part of Greenland at the front of the Knud Rasmussen Glacier and the use of archive image data for monitoring the condition of this glacier. In these short-term visits to the site, the possibility of using a drone is discussed and the results show not only the flow speed of the glacier but also the shape and structure from a height of up to 200 m. From two overflights near the glacier front at different times, it was possible to obtain the speed of the glacier flow and the distribution of velocities in the glacier stream. The technology uses a comparison of two point clouds derived from a set of aerial photos taken with the eBee drone, and calculating the M3C2 (Multiscale Model-to-Model Cloud Comparison) distances with CloudCompare software. The results correlate with other measurement methods like accurate and long-term measurement with Global Navigation Satellite System (GNSS), satellite radar, or ground geodetical technology. The resulting speed from the drone data reached in the middle part of the glacier, was approximately 12–15m per day. The second part of the paper focuses on the analysis of modern satellite images of the Knud Rasmussen Glacier from Google Earth (Landsat series 1984–2016) and Sentinel 2a, and a comparison with historical aerial images from 1932 to 1933. Historical images were processed photogrammetrically into a three-dimensional (3D) model. Finally, orthogonalized image data from three systems (drone photos, historical aerial photos, and satellite data) were compared in the ArcGIS software. This allows us to analyze glacier changes over time in the time span from 1932 to 2020, with the caveat that from 1933 to 1983 we did not have data at our disposal. The result shows that more significant changes in the area of this glacier occurred after 2011. The main aim of this article is to research the use of photogrammetric methods for monitoring the condition and parameters of glaciers based on non-traditional technology, such as drones or new processing of historical photos.
Deployment and Dynamics of a Biofeedback System for Anxiety Awareness during Online Examination Activities
Authors: Apostolidis Hippokratis , Papantoniou Georgia , Tsiatsos Thrasyvoulos
Published: January 2021

The presented paper examines the deployment of a cost-effective biofeedback system that provides anxiety awareness during online examination activities. Human anxiety is classified by evaluating biosignals related to skin conductance, skin temperature, and heart rate. The first aim of this study is to check the presented system performance. Thus, we test the convergent validity of the system regarding self-report measures of anxiety. Moreover, the system is validated against a commercial tool of anxiety detection. Fifteen (15) postgraduate students took part in the relevant psychometric test. The convergent validity of the system is found to be satisfactory. The second aim of this study is to identify the participant’s personality dimensions according to Technology Readiness Index (TRI) which affects their academic performance and their real-time anxiety, as provided by the biofeedback device, during academic examinations. Thirty-five (35) postgraduate students, who were taking examinations in the form of synchronous online tests in the classroom for one of their lessons, took part in this stage of the research. The examined relationships are presented via a path model showing mainly that insecurity causes academic performance to decline, which in turn has a significant negative effect with increasing anxiety.
On New Ideas for Design of Road Infrastructure: Hybrid Fatigue Analyses
Authors: Horr Amir M.
Published: January 2021

To increase the pace of the design of safer road infrastructure and raise the active and passive safety features of road structures on the global stage, innovative and smart virtual tools are essential. One of the basic steps for such ground breaking numerical simulation technology would be to develop advanced smart hybrid techniques with dynamic adaptation into mainstream design and simulation tools that are used by engineering offices. In the research work herein, a new numerical framework including dynamic zoning, advanced grid interfacing, new computationally-efficient solvers, and genetic algorithm symbolic-regression has briefly been presented to address long-standing problems of speed, accuracy, and reliability of numerical tools. The fundamental physical and mathematical aspects of the new simulation framework are concisely presented. In addition, some outcomes of real-world case studies utilized using the proposed hybrid analytical and data-driven (i.e., machine learning, ML) scheme have been shown, where the design rule for road gantry structures is interrogated using the developed virtual tool. One of the main contributions of this paper is to show the benefits of using hybrid simulation technologies to model engineering systems along with the ML-based method to optimize their designs.
Deep Learning-Based Computer-Aided Diagnosis System for Gastroscopy Image Classification Using Synthetic Data
Authors: Kim Yun-ji , Cho Hyun Chin , Cho Hyun-chong
Published: January 2021

Gastric cancer has a high mortality rate worldwide, but it can be prevented with early detection through regular gastroscopy. Herein, we propose a deep learning-based computer-aided diagnosis (CADx) system applying data augmentation to help doctors classify gastroscopy images as normal or abnormal. To improve the performance of deep learning, a large amount of training data are required. However, the collection of medical data, owing to their nature, is highly expensive and time consuming. Therefore, data were generated through deep convolutional generative adversarial networks (DCGAN), and 25 augmentation policies optimized for the CIFAR-10 dataset were implemented through AutoAugment to augment the data. Accordingly, a gastroscopy image was augmented, only high-quality images were selected through an image quality-measurement method, and gastroscopy images were classified as normal or abnormal through the Xception network. We compared the performances of the original training dataset, which did not improve, the dataset generated through the DCGAN, the dataset augmented through the augmentation policies of CIFAR-10, and the dataset combining the two methods. The dataset combining the two methods delivered the best performance in terms of accuracy (0.851) and achieved an improvement of 0.06 over the original training dataset. We confirmed that augmenting data through the DCGAN and CIFAR-10 augmentation policies is most suitable for the classification model for normal and abnormal gastric endoscopy images. The proposed method not only solves the medical-data problem but also improves the accuracy of gastric disease diagnosis.