bluuebunny/crossref_metadata_2025 huggingface dataset:
Binding modes of CYP106A2 redox partners determine differences in progesterone hydroxylation product patterns
Authors: Sagadin Tanja , Riehm Jan L. , Milhim Mohammed , Hutter Michael C. , Bernhardt Rita
Published: July 2018

Natural redox partners of bacterial cytochrome P450s (P450s) are mostly unknown. Therefore, substrate conversions are performed with heterologous redox partners; in the case of CYP106A2 from Bacillus megaterium ATCC 13368, bovine adrenodoxin (Adx) and adrenodoxin reductase (AdR). Our aim was to optimize the redox system for CYP106A2 for improved product formation by testing 11 different combinations of redox partners. We found that electron transfer protein 1(516–618) showed the highest yield of the main product, 15β-hydroxyprogesterone, and, furthermore, produced a reduced amount of unwanted polyhydroxylated side products. Molecular protein–protein docking indicated that this is caused by subtle structural changes leading to alternative binding modes of both redox enzymes. Stopped-flow measurements analyzing the CYP106A2 reduction and showing substantial differences in the apparent rate constants supported this conclusion. The study provides for the first time to our knowledge rational explanations for differences in product patterns of a cytochrome P450 caused by difference in the binding mode of the redox partners.
Development of an electrochemical surface-enhanced Raman spectroscopy (EC-SERS) fabric-based plasmonic sensor for point-of-care diagnostics
Authors: Bindesri Shruti D. , Alhatab Dalal S. , Brosseau Christa L.
Published: July 2018

A plasmonic fabric-based electrode has been developed for wearable EC-SERS sensing for point-of-care applications.
Guilty and charged: a stable solution of the hexamethylbenzene radical cation as a ligand forming oxidising agent
Authors: Schorpp Marcel , Rein Stephan , Weber Stefan , Scherer Harald , Krossing Ingo
Published: July 2018

Arene radical cations as oxidising agents with intended non-innocent behaviour for the synthesis of subvalent Group 13 complex salts.
A turn-on luminescent europium probe for cyanide detection in water
Authors: Huang Sheng-Yin , Pierre Valérie C.
Published: July 2018

A europium complex functions as a turn-on luminescent probe for cyanide in pure water via coordination of the anion.
Two metal–organic frameworks based on pyridyl–tricarboxylate ligands as size-selective catalysts for solvent-free cyanosilylation reaction
Authors: Zhang Yiwen , Su Kongzhao , Hao Miao , Liu Lin , Han Zheng-Bo , Yuan Daqiang
Published: July 2018

Two novel 3D metal–organic frameworks based on multifunctional pyridyl–tricarboxylate ligands as size-selective heterogeneous catalysts for the cyanosilylation of acetaldehydes.
Rational design of CNTs with encapsulated Co nanospheres as superior acid- and base-resistant microwave absorbers
Authors: Liang Chen , Yu Ying , Chen Cailing , Lou Yue , Wang Lei , Liu Kang , Chen Xiao-Bo , Li Chunguang , Shi Zhan
Published: July 2018

A Co@CNT material with a specific coating structure displays good EM wave absorption, even after treatment with concentrated acid or base.
Palladium nanodendrites uniformly deposited on the surface of polymers as an efficient and recyclable catalyst for direct drug modificationvia Z-selective semihydrogenation of alkynes
Authors: García-Calvo José , Calvo-Gredilla Patricia , Vallejos Saúl , García José Miguel , Cuevas-Vicario José Vicente , García-Herbosa Gabriel , Avella Manuel , Torroba Tomás
Published: July 2018

A supported palladium nanoparticle material catalyzes the semihydrogenation of internal alkynes to (Z)-alkenes.
Enhancing the output performance of hybrid nanogenerators based on Al-doped BaTiO3 composite films: a self-powered utility system for portable electronics
Authors: Dudem Bhaskar , Bharat L. Krishna , Patnam Harishkumarreddy , Mule Anki Reddy , Yu Jae Su
Published: July 2018

A composite film consisting of Al-doped BaTiO3 particles with high ferroelectricity is used to enhance the output performance of nanogenerators.
Magnesium-regulated oxygen vacancies of nickel layered double hydroxides for electrocatalytic water oxidation
Authors: Chen Liyong , Zhang Yingyue , Li Dezhi , Wang Yanxin , Duan Chunying
Published: July 2018

Mg2+ cation-regulated generation of oxygen vacancies greatly improves the activity of Ni catalytic sites in NiMg-LDHs for the electrocatalytic OER.
A hydride composite featuring mutual destabilisation and reversible boron exchange: Ca(BH4)2–Mg2NiH4
Authors: Bergemann N. , Pistidda C. , Milanese C. , Aramini M. , Huotari S. , Nolis P. , Santoru A. , Chierotti M. R. , Chaudhary A.-L. , Baro M. D. , Klassen T. , Dornheim M.
Published: July 2018

The formation of MgNi2.5B2 upon dehydrogenation allows a reversible transfer of the boron atoms between the starting reactants and products.
An ultrafast rechargeable lithium metal battery
Authors: Li Xiang , Guo Shaohua , Deng Han , Jiang KeZhu , Qiao Yu , Ishida Masayoshi , Zhou Haoshen
Published: July 2018

We have constructed an artificial organic/inorganic protective layer for typical lithium anodes via pre-treatment in an Mn(NO3)2-containing carbonate electrolyte.
A hidden symmetry-broken phase of MoS2 revealed as a superior photovoltaic material
Authors: Xu Meiling , Chen Yue , Xiong Fen , Wang Jianyun , Liu Yanhui , Lv Jian , Li Yinwei , Wang Yanchao , Chen Zhongfang , Ma Yanming
Published: July 2018

We predict a novel 1Td-MoS2 with a direct band gap of 1.27 eV which reaches a high photovoltaic efficiency of ∼33.3%.
Graphene-based cellular materials with extremely low density and high pressure sensitivity based on self-assembled graphene oxide liquid crystals
Authors: Wu Xianzhang , Hou Kaiming , Huang Jingxia , Wang Jinqing , Yang Shengrong
Published: July 2018

A flexible strain sensor based on an ultralow density cellular material exhibits extremely high sensitivity.
Cyclic boron esterification: screening organic room temperature phosphorescent and mechanoluminescent materials
Authors: Zhang Kai , Sun Qikun , Tang Liangliang , Wang Yanhui , Fan Xiaojing , Liu Linxi , Xue Shanfeng , Yang Wenjun
Published: July 2018

Cyclic esterification of arylboronic acids with diols is a promising avenue for screening organic room temperature phosphorescent and mechanoluminescent materials.
A multiplex loop-mediated isothermal amplification assay for rapid screening of Acinetobacter baumannii and D carbapenemase OXA-23 gene
Authors: Yang Rungong , Zhang Honghong , Li Xiaoxia , Ye Ling , Gong Meiliang , Yang Jinghui , Yu Jihong , Bai Jie
Published: July 2018

Background: Acinetobacter baumannii is a health burden responsible for various nosocomial infections, and bacteremia in particular. The resistance of A. baumannii to most antibiotics including carbapenem has increased. OXA-23-producing A. baumannii is the chief source of nosocomial outbreaks with carbapenem-resistant A. baumannii. Successful antibiotic treatment relies on the accurate and rapid identification of infectious agents and drug resistance. Here, we describe a multiplex loop-mediated isothermal amplification (LAMP) assay for simultaneous and homogeneous identification for A. baumannii infection screening and drug-resistance gene detection. Methods: Four primer pairs were designed to amplify fragments of the recA gene of A. baumannii and the oxa-23 gene. The reaction with a 25 μl of final volume was performed at 63°C for 60 min. For comparative purposes, we used a traditional method of bacterial identification to evaluate assay efficacy. Results: The multiplex LAMP assay enables simultaneous and homogeneous detection of the recA gene of A. baumannii and the oxa-23 gene and requires less than 21 min with no pre-requisite for DNA purification prior to the amplification reaction. The detection is specific to A. baumannii, and the coincidence rate of the multiplex LAMP and the traditional method was 100%. Conclusions: Our data indicate that the multiplex LAMP assay is a rapid, sensitive, simultaneous and homogeneous method for screening of A. baumannii and its drug-resistance gene.
Content pollution propagation in the overlay network of peer‐to‐peer live streaming systems: modelling and analysis
Authors: Wang Haizhou , Chen Xingshu , Wang Wenxian , Chan Mei Ya
Published: July 2018

In the past few years, peer‐to‐peer (P2P) live streaming systems have gained great commercial success and have become a popular way to deliver multimedia content over the Internet, which received more and more attentions from both industry and academia globally. However, the dramatic rise in popularity makes these systems more likely to be vulnerable targets. In this study, mesh‐pull infrastructure architecture and pollution attack principle for P2P live streaming systems were presented firstly, and then the various user behaviours under the pollution attack were analysed. Subsequently, the authors proposed an analytical modelling framework of content pollution attack for P2P live streaming systems. Different from the existing content pollution propagation models, it considers the impact of user behaviours in the attack. Furthermore, to ensure the availability and accuracy of the model, the real‐world experimental attack data for a popular commercial system was used to verify it. The results showed that the model is a feasible and efficient tool to analyse and predict content pollution propagation in real‐world P2P live streaming systems. The authors' work can provide an in‐depth understanding of the content pollution propagation in P2P live streaming systems, and evaluation of restraining illegal content distribution for copyright holders and government.
Output‐based event‐triggered control for networked control systems: tradeoffs between resource utilisation and robustness
Authors: Xie Xuhuan , Li Shanbin , Xu Bugong
Published: July 2018

The additional threshold of a mixed event‐triggering mechanism (ETM) makes it impossible to guarantee the requirement of asymptotic stability. As a consequence, it is necessary to investigate input‐to‐state practically stable (ISpS) which is a mild stability property. In this study, the authors firstly model the closed‐loop event‐triggered control system as a delayed linear parameter‐varying (LPV) systems with a mixed ETM and parameter uncertainty. Then, a co‐design condition in a sense of ISpS of controller gain and trigger parameters for the delayed LPV systems is obtained by employing the parameter‐dependent Lyapunov–Krasovskii functionals and congruent transformation method. Moreover, based on the obtained co‐design condition in a sense of ISpS, an optimisation algorithm for the tradeoffs between resource utilisation and robustness of the closed‐loop system is further proposed. Finally, two numerical examples are provided to demonstrate the effectiveness of the proposed method.
Virtual samples and sparse representation‐based classification algorithm for face recognition
Authors: Peng Yali , Li Lingjun , Liu Shigang , Li Jun , Cao Han
Published: July 2018

Due to the environment and equipment are not controllable, the process of face image acquisition is inevitable to be interfered by external factors, and there are usually only a small number of available face images. Insufficient samples are not conducive to face recognition. Therefore, it is a popular scheme to produce virtual samples based on the available training samples. In this study, the authors first take the symmetry of human face into account, and propose a novel method to generate virtual samples. Then a representation‐based classification method and the score fusion strategy are applied to both original face images and virtual images to perform face recognition. Several sparse representation‐based classification algorithms are compared on ORL, FERET and GT databases. Experimental results show that the authors’ method is effective for improving the face recognition.
Design and control of high‐capacity and low‐speed doubly fed start‐up permanent magnet synchronous motor
Authors: Gao Xinmai , Wang Xuefan , Wei Zhongcao
Published: July 2018

A novel rotor structure with a set of compensation winding for line‐start permanent magnet synchronous motors for high capacity and low speed, which can meet the requirements of a ball mill with a high moment of inertia, is proposed to improve the motor‐starting performance. A control strategy for the compensation winding current is implemented to increase the synchronisation ability with a high‐inertia load. The compensation current and its control method are analysed using a mathematical model and verified using a time‐step finite‐element analysis. The simulation shows that the permanent magnet motor proposed in this study can successfully start at a load of 15 times rotor inertia.
Chromatic transit light curves of disintegrating rocky planets
Authors: Ridden-Harper A. R. , Keller C. U. , Min M. , van Lieshout R. , Snellen I. A. G.
Published: July 2018

Context. Kepler observations have revealed a class of short-period exoplanets, of which Kepler-1520 b is the prototype, which have comet-like dust tails thought to be the result of small, rocky planets losing mass. The shape and chromaticity of the transits constrain the properties of the dust particles originating from the planet’s surface, offering a unique opportunity to probe the composition and geophysics of rocky exoplanets.

Aims. We aim to approximate the average Kepler long-cadence light curve of Kepler-1520 b and investigate how the optical thickness and transit cross section of a general dust tail can affect the observed wavelength dependence and depth of transit light curves.

Methods. We developed a new 3D model that ejects sublimating particles from the planet surface to build up a dust tail, assuming it to be optically thin, and used 3D radiative transfer computations that fully treat scattering using the distribution of hollow spheres (DHS) method, to generate transit light curves between 0.45 and 2.5 μm.

Results. We show that the transit depth is wavelength independent of optically thick tails, potentially explaining why only some observations indicate a wavelength dependence. From the 3D nature of our simulated tails, we show that their transit cross sections are related to the component of particle ejection velocity perpendicular to the planets orbital plane and use this to derive a minimum ejection velocity of 1.2 km s−1. To fit the average transit depth of Kepler-1520 b of 0.87%, we require a high dust mass-loss rate of 7−80 M⊕ Gyr−1 which implies planet lifetimes that may be inconsistent with the observed sample. Therefore, these mass loss rates should be considered to be upper limits.
Research on the influence of a new tap drill geometry on C45, 42CrMo4 and X5CrNi8 steel processing
Authors: Faur Adrian Sorin , Popa Marcel Sabin , Luca Bogdan Cornel , Voina Ioan-Doru , Bizubac Dan
Published: July 2018

The shearing of the chips in a blind hole thread presents a particular problem in machining industry. The tap geometry plays an important role in the tool's durability, influencing the forces during the tapping process and also the way in which the chips are evacuated. This paper analyzes the influence of the tap geometry on the cutting of materials such as C45, 42CrMo4 and X5CrNi8. An adequate geometry will extend the tap durability by controlling the chip when the tap reverses while still engaged in the tapping process. It relates to the improvement of a spiral tap drill, which discharges chips towards the shank via a helical flute. The article contains details about the tap geometries and the results obtained in this research. Based on the results obtained in this research, the purpose of this paper was achieved. The tap durability was improved, the chip deposition on the rake surface was reduced and the torsion torque has a smaller value compared to the standard tap geometry.
The optimal dimensional design of machining technologies
Authors: Toca Alexei , Stroncea Aurel , Stîngaci Ion , Ruşica Ion
Published: July 2018

The paper discusses the design methodology of optimal technological processes of machining, bearing in mind that these processes are related to discrete processes. Discreteness is manifested at different levels: the process consists of a certain number of operations, the operation consists of a number of adjustments and the adjustment consists of a number of operation elements. Each of these levels is a dimensional object with its own internal dimensional structure. The links between dimensional levels are complex and the process is iterative with the ability to return to a higher level.
Experimental research on the elastic deformation mode of 1C45 (1.0503) SR EN 10083- 1.2 rolled steel fastened between the centers of an universal lathe
Authors: Tăbăcaru Lucian , Muscă Gavril , Dzedzid Alexandra
Published: July 2018

Elastic deformations of the technological system occur during the mechanical treatment of a blank, regardless of the manner in which it is fastened. The elastic deformation of the blank is significant especially when machining shaft-like parts. The purpose of our research is to compare the mathematical model of blank deformation to the experimental model when the blank, which is a part belonging to the shaft class, is fastened between centers.
Influence of the cutting edge microgeometry in drilling operation of 42CrMo4 and X5CrNi18-10
Authors: Tomoiagă Vlad-Bogdan , Popa Marcel Sabin , Sattel Stefan , Conţiu Glad , Vereş Ovidiu Virgil , Bozga Marius
Published: July 2018

It's known that the durability of the cutting tool is influenced by the cutting parameters. In the recent years, scientists turn their research efforts to demonstrate that the microgeometry have an important role in determining the durability of the cutting tools. The processes studied most are milling and turning, but few researches have been done on the drilling operation. In this paper is presented the influence of 6.8 mm diameter drills with three values of the K-factor, but the same radius of the cutting edge and same macrogeometry. To obtain the desired microgeometry of the drills, two preparing technologies of the cutting edge were used. Drills were tested in two types of materials: X5CrNi18-10 (1.4301) and 42CrMo4 (1.7225). The main goal of this paper is to observe the influence of the microgeometry in the drilling process namely the durability and wear evolution.
A study for determining the most significant parameters of the ball-burnishing process over some roughness parameters of planar surfaces carried out on CNC milling machine
Authors: Slavov Stoyan D. , Dimitrov Diyan M.
Published: July 2018

Ball burnishing process often applies for obtaining specifically characteristics in the surface layer of the functional surfaces of the machine parts. Using CNC machines for carrying out this process significantly increases the possibility of control the characteristics of the toolpath of the ball tool and thus to produce regularly distributed roughness with specific size and direction of cells alignment. The current study presents obtained results from L8 Taguchi experimental design for determining the significantly influenced parameters of the ball burnishing process carried out on CNC milling machine on the size and arrangement direction of the cells form regular distributed roughness on planar surfaces.
Comparative study on microhardness between friction stir welding and tungsten inert gas assisted friction stir welding of pure copper
Authors: Constantin Marius Adrian , Boşneag Ana , Niţu Eduard , Iordache Monica
Published: July 2018

Welding copper and its alloys is usually difficult to achieve by conventional fusion welding processes because of high thermal diffusivity of copper, which is at least 10 times higher than most steel alloys. In order to reduce the increased temperature loss, it would be advantageous to use a process that is carried out at lower temperatures. Friction Stir Welding (FSW) is a solid state joining process that involves the joining of two metal pieces at the molecular level without melting and was explored as a feasible welding process. In order to achieve an increased welding speed and a reduction in tool wear, this process is assisted by another one (TIG - tungsten inert gas) which generates and adds heat to the process. The research includes two experiments for the FSW process and two experiments for TIG assisted FSW process. It is presented the evolution of the temperature and of the axial force during the process and is determined the microhardness for each experimental case. The aim of this paper is to make known the effects of using TIG assisted FSW process on the microhardness of the pure copper joints and to present some conditions in which it is less affected.
Studies on robotic testing equipment used in mechatronic devices manufacturing processes to improve the root cause analysis
Authors: Braga Ion Cristian , Nedelcu Anisor , Udroiu Razvan
Published: July 2018

In the newest technology of Industry 4.0 and more complex products manufactured, as the innovation requires new flexible manufacturing equipment using robots and complex designed workstations, and the robotic testing equipment as well, the engineers are faced with new challenges to the processing of the information, multiple parameters being collected. The paper aim is to present the studies performed on robotic testing equipment from a manufacturing line of an automotive mechatronic device by using the design of experiments to optimize the 3D movements of the arm, the speed and approach procedure of the testing device. The ANOVA will support the analysis of the repeatability of the movements and find the main factors which lead to variation in measurements, therefore could support the root cause analysis of issues occurred.
A new role of unruly product design based on “Transformed Objects” design approach
Authors: Manavis Athanasios , Kapakiari Nikoleta , Oancea Gheorghe
Published: July 2018

Nowadays, the movement of Post-Modernism, which appeared in 20th century, influences the art and the design of products. It is considered that the investigation and the undermining, which construct the modern society, compel a traditional prioritization based on cultural values. Furthermore, today the main effect is the image as the users opt to purchase their products by the appearance that is more substantial than the usage. For that reason, the alteration of daily products is the cause of this paper so that the consumers feel confused by them. Namely the basic image of a product may be the same as or similar to an original object, but it may alter the use of it. The viewer should interpret the image differently such as his experience and his belief. In addition, the change of visual messages can be achieved by the complexity of geometry, the combination of objects and the modification of materials. This is defined by the movement of Unruly design that aims for offering a new aesthetic of products. Finally, the goal of this paper is to present a number of illustrative case studies, which ensure an appropriate interface relationship between the art and product design.
Actions to increase the reliability of the chain transmissions
Authors: Ghimişi Ştefan , Nicula Dana
Published: July 2018

Correct analysis of a malfunction is a very important step in the remediation process of a product. It is necessary to have a clear vision, especially to determine the cause of the malfunction, although sometimes it can be masked by other defects. Quality Management offers many classic and modern tools to help find solutions that ensure quality improvement. This paper presents a modern method of quality management, called the tree diagram, applied to the issue of increasing the reliability of the chain transmissions. The advantage of using this chart is that it offers the possibility of logically and chronologically examining the objectives and actions that solve a quality or non-quality problem. In the proposed case study, the overall objective is to increase the reliability of the chain transmissions. Specific objectives are set and for each specific objective, we identify potential solutions that can be adopted. For the proposed general objective, the following three specific objectives have been identified: Reduction of chain wheel wear and chain joints, Ensuring proper fitting, Adoption of optimal chain tensioning solutions. On the basis of the presented measures, the tree diagram was made on three levels. The tree chart made outlines the relationship between the overall goal pursued and the actions taken to achieve them. This tool can be used in planning and solving quality assurance problems in the case of the chain transmissions.
Fatigue life investigation on a MAC engine piston
Authors: Humelnicu Costel , Amortila Valentin , Mereuta Elena
Published: July 2018

In MAC (Diesel) engines case, the shape of the piston head has an important influence on burning processes and, consequently on engine performance. Since the piston is subjected to high cyclic stresses during the blasting phase, a fatigue phenomenon occur. The piston design and material must provide a high fatigue resistance and a low weight, leading to low dynamic loading of the crank shaft and piston rod. The paper try to investigate the piston fatigue behavior, for several head shapes and materials, in order to establish an optimal design.
Establishing the torsional rigidity of a rotation module responsible for the flexion-extension motions of the elbow
Authors: Deaconescu Tudor , Deaconescu Andrea
Published: July 2018

The paper presents and discusses a system devised for the mobilisation of the elbow including a pair of agonist-antagonist pneumatic muscles aimed mainly at ensuring the accuracy of the rotation angle. This system is of SISO type (single input - single output) with the variable Δp (the pressure by that one of the muscles is charged symmetrically in the detriment of the other) as its input value. Further discussed are the dependency of the equilibrium angle on Δp and the possibility of controlling the torsional rigidity by adjusting the sum of the feed pressures.
Measurement loop, automatic tuning and product quality tracking
Authors: Man Dietrich Marcela , Popa Marcel Sabin
Published: July 2018

Starting from the PDCA cycle (Plan - Do - Check - Act), imposed by the ISO 9000 quality standards, a loop is proposed to automatically adjust the work equipment to the statistical distribution of the quality feature of the last N products randomly selected. The loop promptly warns the leadership in case of distortion of the statistical distribution, caused by equipment, raw materials, human operators or the environment. It also constantly informs about the percentage of noncompliant products. The main original element, at the heart of the article's concerns, is the quality tracking loop presented in section 4. The starting point is the quality management scheme, according to ISO9000, while sections 2-3 expose the theoretical basis of the subject, without which it would have not been possible to deepen the role of the modules from the loop proposed in Figure 6.
Improving the quality management of an enterprise in the field of underwater construction
Authors: Plakhin Andrey , Semenets Igor , Kokovikhin Alexandr , Dolzhenko Ruslan
Published: July 2018

Quality management is the basis for achieving the competitive advantages of any enterprise. The article reveals the necessity of research into the problem of quality management with the aim of forming competitive advantages of the enterprise. The authors consider the solution of this problem by the example of an underwater construction enterprise.
Intérêt diagnostique et thérapeutique de l’étude du nez en orthopédie dento-maxillo-faciale
Authors: Salagnac Jean-Michel
Published: July 2018

Introduction : Prévoir la croissance faciale chez un sujet en période de croissance est l’un des souhaits les plus chers des orthodontistes, mais aussi l’un des objectifs les plus difficiles à obtenir avec précision malgré les différentes méthodes proposées de prévision de croissance. Une méthode simple basée sur la lecture clinique et radiologique du nez et du prémaxillaire permet sans aucun calcul de se faire une idée valable de la croissance maxillaire à venir et d’en déduire les indications thérapeutiques en orthopédie dento-faciale. L’étude morphologique du nez constitue également un élément important dans le diagnostic des syndromes malformatifs cranio-faciaux. Matériels et méthodes : À partir d’une étude sémiologique clinique détaillée et de l’étude radiologique des structures nasales et prémaxillaires, cet article présente une méthode d’évaluation et de prévision de croissance de l’étage supérieur de la face. Résultats : Basée sur l’expérience de très nombreuses observations, et sur des bases embryologiques actuelles, la détection précoce des troubles de la croissance de l’entité ethmoïdo-naso-prémaxillaire fournit à l’orthodontiste de précieuses indications thérapeutiques. Discussion : L’étude de la morphologie nasale, clinique et radiologique associée à l’étude du prémaxillaire constitue un bon indicateur de la direction et du potentiel de croissance de l’étage supérieur de la face, ainsi qu’un indice de dépistage de syndrome malformatif. Conclusion : Cette étude devrait être intégrée systématiquement dans le diagnostic en orthopédie dento-maxillo-faciale.
Nowcasting of Solar Energetic Particle Events using near real-time Coronal Mass Ejection characteristics in the framework of the FORSPEF tool
Authors: Papaioannou Athanasios , Anastasiadis Anastasios , Sandberg Ingmar , Jiggens Piers
Published: July 2018

In this work the derived occurrence probability of solar energetic particle (SEP) events (i.e. proton events measured at Earth’s position) and their peak fluxes and total fluences depending on coronal mass ejection (CME) parameters, i.e. linear speed (V) and the angular width (AW) are presented. A new SEP catalogue with associated CME data from 1997 to 2013 is utilized. It is found that the SEP probability strongly depends on the CME speed and the angular width as follows: The highest association (72.70%) is obtained for the full halo CMEs with V ≥ 1500 km s−1 and the lowest association (0.7%) is found for the non halo CMEs with 400 km s−1 ≤ V ≤ 1000 km s−1. The SEP occurrence probabilities are different as much as 26 times according to the CME speed (V), comparing fast versus slow CMEs and 44 times according to the AW, comparing halo to non halo CMEs. Furthermore, linear regressions of the proton peak flux and integral fluence at several integral energy channels (E > 10 MeV, E > 30 MeV, E > 60 MeV, E > 100 MeV) were obtained. Our results, were used to build a module of an operational forecasting tool (i.e. FORecasting Solar Particle Events and Flares – FORSPEF, http://tromos.space.noa.gr/forspef/). This module performs nowcasting (short term forecasting) of SEP events using near real-time CME identifications obtained from CACTus (http://sidc.oma.be/cactus/). The outputs offered by the operational module of the tool to the end user (textural, pictorial, archived data) are presented. Finally, the validation of the system, in terms of archived data is described, in terms of categorical scores (Probability of Detection – POD and a False Alarm Rate – FAR).
Eigengefährdung des Patienten ernst nehmen
Authors: Kemmel Markus
Published: July 2018

Im siebten Teil unserer Serie geht es um einen Patienten mit paranoider Schizophrenie, der immer wieder Suizidgedanken äußert. Aufgrund seiner langen Aufenthalte und seines ablehnenden Verhaltens wird die potenzielle Selbstgefährdung des Patienten vom Stationsteam nicht hundertprozentig ernst genommen. Die Motivation, ihm zu helfen, ist gesunken. Lesen Sie, wie das Pflegeteam es schafft, mithilfe der systemischen Fallbesprechung neue Perspektiven zu entwickeln. Auch hier möchten wir Sie wieder einladen, sich selbst in die verschiedenen Rollen hineinzuversetzen. Fallen Ihnen weitere Lösungswege ein? Dann schreiben Sie uns an psych.pflegeheute@thieme.de.
Bauaktivitäten deutscher Kliniken: Leichter Anstieg trotz unsicherer Zukunft
Authors: Kucera Martin
Published: July 2018

Die Zahl der Krankenhäuser in Deutschland sinkt stetig. Wie steht es mit den Bauaktivitäten im Gesundheitswesen? Die Münchner Unternehmensberatung Schwab Marketing registriert in ihrer aktuellen Marktstudie für 2018 etwas mehr Baumaßnahmen als noch vor einem Jahr.
Bettenreinigung: Kooperation für optimale Hygiene
Authors: Kucera Martin
Published: July 2018

Zu den Hygienemaßnahmen im Krankenhaus gehört auch das Reinigen und Desinfizieren der Bettgestelle. Immer mehr Kliniken entscheiden sich hier für die maschinelle Aufbereitung. Einige Bettenhersteller haben deshalb Kooperationen mit den Herstellern solcher Anlagen geschlossen, um den Reinigungsprozess zu optimieren.
Wegeleitsysteme im Krankenhaus: Ein kluges Orientierungssystem entlastet die Pflegekräfte
Authors: Kucera Martin
Published: July 2018

Wegeleitsysteme sind eine besondere Herausforderung – gerade in Gesundheitsbauten, in denen nicht nur Patienten, sondern auch Besucher und Mitarbeiter schnelle, klare Orientierung benötigen. Im Gespräch mit kma erläutert Dr. Sylvia Blezinger, Geschäftsführerin des auf Dienstleistungen spezialisierten Unternehmens Blezinger Healthcare, welche Knackpunkte es hier zu beachten gilt.
Überraschender Abgang: Charitésprecher Dolderer geht „einvernehmlich“
Authors: Müller Herrmann
Published: July 2018

Uwe Dolderer, seit August 2013 Pressesprecher und Leiter der Unternehmenskommunikation, hat die Berliner Charité überraschend verlassen. Dolderer wolle sich „neuen beruflichen Herausforderungen“ stellen, so die stellvertretende Sprecherin Manuela Zingl auf Anfrage von kma. Man bedauere den Weggang, alles sei einvernehmlich erfolgt.
cdgw-Zukunftspreis 2018: Innovationen treffen auf Anwender
Authors: Emmrich Michael
Published: July 2018

Die Bewerbungsfrist für den Deutschen Zukunftspreis des Clubs der Gesundheitswirtschaft (cdgw) endet am 15. September 2018. Noch sind also Einsendungen möglich. Eine Jury wählt aus den Einsendungen maximal sechs Teilnehmer für den Wettbewerb aus. Er findet am 6. November im Internationalen Club im Auswärtigen Amt in Berlin statt.
Studies of the Factor Xa-Dependent Inhibitor of Factor VIIa/Tissue Factor (Extrinsic Pathway Inhibitor) from Cell Supernates of Cultured Human Umbilical Vein Endothelial Cells
Authors: Warn-Cramer Bonnie J , Almus Fanny E , Rapaport Samuel I
Published: July 2018

Cultured human umbilical vein endothelial cells (HUVEC) have been reported to produce extrinsic pathway inhibitor (EPI), the factor Xa-dependent inhibitor of factor VHa/tissue factor (TF). We examined the release of this inhibitor from HUVEC as a function of their growth state and in response to the induction of endothelial cell TF activity. HUVEC constitutively produced significant amounts of EPI at all stages of their growth in culture including the post-confluent state. Rate of release varied over a 3-fold range for primary cultures from 12 different batches of pooled umbilical cord cells. Constitutive EPI release was unaltered during a 6 hour period of induction of TF activity with thrombin or phorbol ester but slowed during longer incubation of the cells with phorbol ester. Whereas plasma contains two molecular weight forms of EPI, only the higher of these two molecular weight forms was demonstrable by Western analysis of HUVEC supernatants with 125I-factor Xa as the ligand.
Haemostatic Effects of Recombinant Human Erythropoietin in Chronic Haemodialysis Patients
Authors: Geet Chris van , Hauglustaine Didier , Verresen Luc , Vanrusselt Marleen , Vermylen Jos
Published: July 2018

Recombinant human erythropoietin was administered for up to 40 weeks to nine patients on chronic haemodialysis. From the third week of administration onwards, not only haemoglobin and haematocrit but also the platelet count rose, the latter, however, transiently. Subnormal platelet aggregation before therapy also improved transiently and in parallel with the erythropoietin dosage. The bleeding time normalized in almost all patients. There were no major side-effects. We conclude that recombinant erythropoietin improves haemostasis in chronic haemodialysis patients by increasing the haematocrit and in addition transiently enhances platelet number and function.
Effects of Calcium Channel Blockers on In Vitro Platelet Function in Whole Blood Using Single Platelet Counting
Authors: Walley T J , Woods K L , Barnett D B
Published: July 2018

The effects of various calcium channel blockers on in vitro platelet aggregation were studied using whole blood platelet counting. Diltiazem and verapamil inhibited aggregation caused by adrenaline 5 μM and collagen 2 μg/ml, but only at concentrations far in excess of those seen in clinical use. Nifedipine and nimodipine also inhibited aggregation to these agonists, but this seemed to be due to the solvent used. The solvent for these dihydropyridines contains 15% ethanol, 15% polyethylene glycol and 70% water, and the ethanol component is the most likely to have caused an antiaggregatory effect. Other studies showing an antiaggregatory effect of dihydropyridines may not have taken account of this solvent effect.
The Effect of Va rious Anticoagulant/Anti platelet Mixtures on Determination of Plasminogen Activator Inhibitor, Platelet Proteins and Hemostasis Farameters
Authors: Eriksson E , Lengborn L , Risberg B
Published: July 2018

Blood collected in different anticoagulant/antiplatelet agents(ETP, EDTA, citrate, citratelcitric acid pH 4.5 and CTAD) was compared with respect to determination of PAI-1 activity and PAI-1 antigen. βTG and PF4 were analysed as markers of platelet release. Both the middle layer and the remaining layer of the plasma were studied. Moreover vWF:Ag, FVII:Ag, ECLI t-PA:Ag, t-PA activity, APTT VIII: C and VII: C were assayed in blood collected in citrate and CTAD

PAI-1 activity showed the same level in all citrate based anticoagulants and ETP and no increase was found in blood standing for 2 hours at room temperature. On the contrary quick handling was most important for determination of PAI-1 antigen. In tubes anticoagulated with citrate no significant increase was found if the sample was prepared within 1 hour. EDTA was not suitable as anticoagulant mixture. Tubes containing the antiplatelet mixture CTAD could be used for determination of PAI activity, PAI antigen, vWF:Ag, FVIIiAg, t-PA activity and APTT.

For measurement of PAI-1 antigen quick handling of blood anticoagulated with antiplatelet mixtures are preferable, and plasma treated in that manner could also be used to assay some hemostasis parameters.
A Thiocholine Ester of Cinnamic Acid Inhibits Crosslinking of Fibrin without Specific Binding to Donor Lysines
Authors: Perret B A , Seelich T , Furlan M , Beck E A
Published: July 2018

In the presence of activated factor XIII, 2-diethylbenzyl-aminoethylthiol-14C-transcinnamate bromide completely inhibited the crosslinking of fibrin. However, all three fibrin chains bound the cinnamic acid, and, in the α- and γ-chains, the binding of label was not restricted to the crosslinking donor sites as might be expected. Furthermore, even in the absence of activated factor XIII, fibrinogen and fibrin incorporated cinnamic acid. Thus, as well as reacting with the functional thiol group of factor XIII, the thiocholine ester of cinnamic acid is incorporated non-specifically throughout the fibrinogen and fibrin subunit chains. The thiocholine ester used differs in this respect from dansyl cadaverine which is incorporated enzymatically and exclusively to the (acceptor) sites involved in crosslinking. Thiocholine ester of cinnamic acid cannot be used as a label for localization of specific crosslinking donor sites.
Increase of the Sialic Acid-Rich Variants of the Bβ-and γ-Chains of Fibrinogen Zurich II
Authors: Straub P W , Gati W P
Published: July 2018

The abnormally high sialic acid-content of fibrinogen Zurich II could be attributed to the presence of a higher proportion of the sialic acid-rich L variant of both the γ and Bβ- polypeptide chains of fibrinogen Zurich II as compared to normal fibrinogen.
Is Quantitative Determination of Fibrin(ogen) Degradation Products and Thrombin-Antithrombin III Complexes Useful to Diagnose Deep Venous Thrombosis in Outpatients?
Authors: van Bergen Paul F M M , Knot Eduard A R , Jonker Jan J C , de Boer Auke C , de Maat Moniek P M
Published: July 2018

We studied the diagnostic value of recently introduced ELISA’s for the determination of thrombin-antithrombin III (TAT) complexes, fibrin degradation products (FbDP), fibrinogen degradation products (FgDP) and total degradation products (TDP) for deep venous thrombosis (DVT) in plasma of 239 consecutive outpatients, suspected for DVT by their family doctor. DVT was confirmed by impedance plethysmography in 60 patients. Using the 95th percentile range of 42 healthy volunteers the sensitivity for the detection of DVT was: 37% for TAT, 95% for TDP, 92% for FbDP and 90% for FgDP. Specificity was: 88% for TAT, 16% for TDP, 20% for FbDP and 25% for FgDP.

We conclude that these assays are of little value in the diagnosis of DVT in outpatients.
Association of Reduced Factor VIII with Impaired Platelet Reactivity to Adrenalin and Collagen after Total Thyroidectomy
Authors: Palareti G , Biagi G , Legnani C , Bianchi D , Serra D , Savini R , Coccheri S
Published: July 2018

Twenty-one thyroprival patients, previously submitted to total thyroidectomy for tumours, were investigated during stabilized L-thyroxine supplementation and at the end of a 20-25 day “no-therapy” period, necessary for a 131I total body scintigraphy. During supplementation therapy a lower than normal mean betathromboglobulin (beta-TG) release level was found, the other blood clotting and platelet function tests being normal. After substitution therapy withdrawal, platelet function tests showed reduced adrenalin aggregation, increased collagen threshold aggregating concentrations, decreased beta-TG release values and reduced aggregation to ristocetin, whereas blood clotting tests showed prolonged aPTT values and reduced levels of factor VIII: C and vWf: Ag. We conclude that in acquired hypothyroidism the lowering of factor VIII: C and vWf: Ag (acquired von Wille brand disease) is associated with impaired platelet reactivity not only to ristocetin but also to collagen and especially adrenalin. In the patients investigated these changes were almost completely corrected by substitutive therapy with L-thyroxine at clinically effective doses.
C1-Esterase Inhibitor in Uncomplicated Pregnancy and Mild and Moderate Preeclampsia
Authors: Halbmayer Walter-Michael , Hopmeier Pierre , Mannhalter Christine , Heuss Friedrich , Leodolter Sepp , Rubi Karl , Fischer Michaet
Published: July 2018

Routine coagulation tests and measurement of the plasma levels of C1-esterase inhibitor (C1-INH) and antithrombin III were performed in 17 women with mild preeclampsia, 10 women with moderate preeclampsia and 20 women with uncomplicated pregnancy. All pregnant probands were within the third trimester of pregnancy. 20 non-pregnant women were used as controls. The gfoups were matched in age and the pregnant ones were also matched in gestational age.

C1-INH activity and antigen were significantly reduced (p <0.002) in normally pregnant women as compared with nonpregnant ones. Further, CI-INH activity was lower in women with mild preeclampsia and significantly lower in women with moderate preeclampsia (p <0.05) as compared with normally pregnant women. None of the plasmatic coagulation tests was indicative of a consumption reaction. We conclude that C1-INH activity and antigen reductions are commonly associated with pregnancy. Furthermore, as markedly lower values can be found in mild and moderate preeclampsia, measurement of the C1-INH activity in pregnant women within the third trimester might proof useful to establish the diagnosis of a preeclamptic condition.
A Comparative Study of Crosslinked and Noncrosslinked Fibrin from the Major Classes of Vertebrates
Authors: Schwartz Martin L. , Pizzo Salvatore V. , Sullivan J Bolling , Hill Robert L. , McKee Patrick A.
Published: July 2018

Crosslinked and noncrosslinked fibrin formed by clotting whole plasma in the presence and absence of calcium has been examined by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The fibrin from 45 species of vertebrates with representatives from all classes except the Bradyodonti (Chimaeras) and a majority of the subclasses have been compared. Noncrosslinked fibrin from the mammals contained subunits resembling the α, β, and γ chains of human noncrosslinked fibrin. The molecular weight of the α-chains varied greatly, the molecular weight of the β-chains varied slightly, while the molecular weight of the γ-chains was apparently invariant. Nonerosslinked avian fibrins showed clearly resolved γ-chains with a molecular weight slightly smaller than human γ-chains. Avian α- and β-chains were usually not resolved. Nonerosslinked fibrin from the reptiles and amphibians contained a clearly resolved subunit with a molecular weight similar to that of human γ-chain. In crosslinked fibrin from all of the mammals, birds, reptiles, and amphibians the γ-chain was absent and a new band which corresponded to a dimer of the γ-chain was found, while high molecular weight polymers were only found in a few species. Fibrin formed from the plasma of the bony fishes was often difficult because of the problem of fibrinolysis ; however, non crosslinked fibrin from some species of bony fish had three clearly resolved subunits while the crosslinked fibrin from all of the species examined had dimers as the predominant crosslinked forms. The noncrosslinked fibrin from the cartilaginous fish had subunits in the same molecular weight range as the other vertebrate fibrins; however, the crosslinked fibrin was unique to this class because all of the fibrin subunits were involved in the crosslinking process. Dimers appeared to be a transient species, and a large number of different high molecular weight crosslinked species were formed. The crosslinked fibrin from the hagfish contained dimers and no higher molecular weight crosslinked forms. Rapid fibrinolysis complicated the interpretation of the results from all of the classes of fish.
Inhibition of Injury Induced Thromboatherosclerotic Lesions by Anti-Platelet Serum in Rabbits
Authors: Moore Sean , Friedman R. J , Singal D. P , Gauldie J , Blajchman M. A , Roberts R. S
Published: July 2018

We have previously shown that repeated or continuous intimal injury caused by an indwelling aortic catheter causes a variety of lesions in rabbits maintained on a diet unsupplemented by lipid. These include fatty streaks, lipid-free fibrous plaques and lipid-rich raised thromboatherosclerotic plaques. Whether lipid-rich raised lesions are a result of injury or co-existing thrombosis or both is not clear. The present experiment was designed to answer this question. Anti-platelet serum (APS) to washed sonicated rabbit platelets was raised in sheep. PE 60 polyethylene catheters were placed in the aortas of 35 rabbits by way of a femoral artery. The animals were randomly divided into 2 groups. The experimental group (17 rabbits) received an intravascular injection of 1.0 ml of APS followed 8 hours later by a subcutaneous injection of 0.5 ml. Thereafter, 0.5 ml APS was given subcutaneously each day for 13 additional days. The control group (18 rabbits) received no APS. Platelet counts were done prior to surgery, at 5 minutes following surgery, at 4 days, 8 days and just prior to killing. Extent of lesions was estimated by photographing the opened aortas, projecting the photographs on cardboard, cutting out the areas occupied by the different lesions and weighing the cardboard. The mean weight of raised lesions in the control group was 6 to 7 times greater than in the experimental groups. Statistical analysis of this difference based on Welsh‘s “t” test for unequal variances was highly significant (P<0.001). Platelet counts in the experimental group varied from 0 to 20,000 at 14 days. In animals with platelet counts ≤ 1,000 mm3 raised lesions were completely prevented. In a second experiment the effect of APS was compared with normal sheep serum (NSS). A similarly significant inhibition of raised lesions occurred in the APS group. The extent of lesions in the NSS control was similar to that in the No-APS group of the first experiment. These findings indicate that thrombosis is more important than injury in the development of lipid-rich raised lesions.