bluuebunny/crossref_metadata_2025 huggingface dataset:
Isolation and characterization of one autochthonous Beauveria sp. isolate from Cuban fields
Authors: Pèrez Àlvarez Sandra , Machín Suárez Alianna , Del Pozo Núñez Elio Minel , García Cruz Irma , Héctor Ardisana Eduardo Fidel , Domínguez Yoannis , Guerrero-Morales Sergio , Chávez Medina Jesús Alicia , Escobedo Bonilla César Marcial
Published: March 2022

The genus Beauveria comprises a cosmopolitan teleomorphic and anamorphic group of ento-mopathogenic fungi. The aim of this study was to characterize a new isolate of Beauveria spp. and compare it to the LBB-1 commercial strain. The isolate was collected from the ant Atta insularis and adults of the coffee borer, respectively. It was cultivated on solid SDAY medium enriched with 1% yeast extract. The biological effectiveness of the isolate was determined on C. formicarius. DNA was isolated using the CTAB method and an internal transcribed spacer (ITS1, ITS4) were used as primers for amplification. The consensus sequence obtained was deposited in GenBank (MK246940) and used for phylogenetic analysis. This isolate showed differences in morphological characteristics; however, it showed low genetic variability regarding worldwide isolates of B. bassiana. Concerning its biological effectiveness, the isolate demonstrated to be virulent on the phytophagous adults, producing 50% mortality. Four clades were identified and the polytomous topography recovered for clade 1 indicates high genetic similarity (99-96%) with Beauveria bassiana from several countries. The morphological and molecular characterization made it possible to accurately identify the new isolate as Beauveria bassiana. The identified specie is another possibility for integrated pest management in Cuba and Mexico.
Actividad de tres aceites esenciales sobre la mortalidad de Rhyzopertha dominca (F.) en trigo almacenado
Authors: Borboa Flores Jesús Jesús , Iturralde García Rey David , Borboa Flores Jesùs , Sánchez Maríñez Reyna Isabel , Wong Corral Francisco Javier , Cortez Rocha Mario Onofre , Cinco Moroyoqui Francisco Javier
Published: March 2022

Rhyzopertha dominica (F.) es una plaga que provoca daños significativos en el grano almacenado de trigo y para su control se usa indiscriminadamente sustancias químicas provocando resistencia a insectos, además de problemas ambientales y de salud. Por consiguiente, se evaluó el efecto de los aceites esenciales de Melaleuca viridiflora Gaertn, Lippia palmeri Watson y Eucalyptus camaldulensis Dehnhardt sobre la mortalidad de R. dominica en trigo, utilizándose diversas concentraciones de aceites (0, 5, 10, y 15 μl) y tiempos de exposición (24, 48 y 72 h) sobre R. dominica. Se determinó la repelencia y emergencia del insecto adulto, además la pérdida de peso producido en el grano de trigo. Los resultados obtenidos indican que existe una relación directamente proporcional en el parámetro de mortalidad con respecto al tiempo de exposición y la concentración de los aceites, quedando los aceites en el siguiente orden de importancia M.viridiflora, E. camaldulensis y L. palmeri. De estos tres aceites el que mejor efecto exhibió fue M. viridiflora sobre la mortalidad de R. dominica, la emergencia de insectos adultos y la reducción en la pérdida de peso. Por otro lado, el aceite L. palmeri fue el que mejor repelencia presentó.
The biological meaning of preferences on the human mate market
Authors: Pawłowski Bogusław
Published: March 2022

Factors, which universally influence mate choice decisions, are: age, physical appearance, and resources. Different evidence of the biological relevance of these three factors on the human mate market is presented. The sexspecific preferences for partner.s age reflect such values as Fisherian reproductive potential and fecundity for females and life expectancy related to the length of further time of paternal investment for males. Physical attractiveness is analyzed as a marker of female.s age, and in the case of both sexes as a marker of hormone level, heterozygosity and therefore immunocompetence. Because attractiveness connotes genetic quality of a prospective partner and indicates higher fitness for offspring, it is not an arbitrary trait. Resources of males are discussed as being important for females because they assure good paternal investment and therefore higher reproductive success. Human flexibility in age and physical attractiveness preferences is also examined in relation to ecological conditions. The evidence presented here indicates that the factors influencing human mate choice decisions can be honest, biologically relevant cues of reproductive value of a prospective partner.
The comparison between two age estimation methods based on human teeth
Authors: Żądzińska Elżbieta , Drusini Andrea G. , Carrara Nicola
Published: March 2022

From among age estimation procedures we chose two for comparison. One of them is the standard method based on root dentin transparency; the other one is based on coronal pulp cavity heigth. The material consisted of X-ray photographs and longitudinally sectioned teeth of 276 individuals (174 males and 102 females) of known age. The comparison shows that the poorly known TCI method is at least as precise as the most widely applied and acknowledged procedure based on longitudinal sections of teeth.
Fertility, offspring survival, and adaptive value as related to morphological traits – methodological and interpretation problems
Authors: Kościński Krzysztof
Published: March 2022

A great deal of research has been carried out in order to find a relationship between somatic traits and fertility, offspring survival rate and adaptive value. However, the results of these studies lack consistency. The reason behind these discrepancies is the use of inadequate statistical methods and the application of unreliable principles of interpretation. The purpose of this paper is to indicate the methods of data analysis and result interpretation, which permit optimum utilisation of the information contained in a given sample and make it possible to draw the correct conclusions.
Case study of accelerated wear of brake discs made of grey cast iron characterized by increased thermal stability
Authors: Zemlik Martyna , Dziubek Mateusz , Pyka Dariusz , Konat Łukasz , Grygier Dominika
Published: March 2022

In this paper, the subject of the analysis is Rotinger High Performance brake disc, characterized by increased thermal resistance, which is a result of adequate design and material solutions. However, despite declared performance, the analyzed discs suffered accelerated wear over a period of about 20 months. The aim of this study was to assess the causes of the disc failure. As a result, it was showed that the applied material solution still does not differ from standard ones. According to the authors of this paper, an appropriate procedure to improve resistance to thermal fatigue is, among others, changing the shape of graphite precipitates from flake to spheroidal.
Perbandingan Efektivitas Sacubitril/Valsartan dengan Enalapril pada Pasien Gagal Jantung: Literature Review
Authors: Mumpun Jitayu Sekarininta , Maharani Laksmi , Endriastuti Nialiana Endah
Published: March 2022

ARNI adalah terapi baru untuk pasien gagal jantung yang diakui pada 2016, yang merupakan kombinasi antara sacubitril (Neprilisin Inhibitor) dengan valsartan (ARB). Penelitian ini bertujuan untuk mengetahui perbandingan efektivitas pengunaaan sacubitril/valsartan dengan enalapril pada pasien gagal jantung melalui metode literature review. Pencarian artikel dilakukan pada database Cochrane dan PubMed. Kata kunci yang digunakan disusun berdasarkan PICO (heart failure, sacubitril/valsartan atau LCZ696, enalapril, dan efficacy or effectiveness). Kriteria inklusi pada penelitian yaitu artikel dalam bahasa inggris, full text dan bebas akses, publikasi 2010-2020, dan penelitian RCT. Kriteria eksklusi yaitu artikel review. Pencarian dilakukan pada 10 Juli hingga 23 Juli 2020 dan 20 Desember 2020. Didapatkan hasil pencarian 160 artikel, setelah dilakukan seleksi dan eligibilitas diperoleh 16 artikel sesuai kriteria inklusi sebagai arikel primer, dan 2 artikel ditambahkan sebagai artikel sekunder. Penelitian membuktikan bahwa sacubitril/valsartan menurunkan kejadian readmisi dan memiliki skor kualitas hidup lebih baik dibandingkan enalapril, serta dapat menurunkan resiko kematian. Berdasarkan penelitian primer PARADIGM-HF dan 15 artikel analisis lanjutan, diketahui bahwa sacubitril/valsartan lebih bermanfaat dibandingkan enalapril pada pasien gagal jantung.

Kata kunci: sacubitril/valsartan, enalapril, gagal jantung, literature review
Implementasi Metodologi Six Sigma Untuk Meningkatkan Kualitas Produk Kursi Kuliah
Authors: Aulya Muhamad Faizal , Syukron Amin , Soolany Christian
Published: March 2022

Six sigma merupakan cara pendekatan kualitas terhadap Total Quality Management (TQM). TQM menjadi perhatian di Amerika Serikat tahun 80-an dan ini merupakan suatu respons terhadap superioritas kualitas dari pabrikan Jepang dalam bidang automotif dan penyejuk ruangan. Banyak studi pada bidang penyejuk ruangan mengemukakan bahwa kerusakan (defect) pada perusahaan Amerika Serikat lebih banyak dari perusahaan Jepang. Untuk membantu perusahaan supaya mampu memperbaiki program peningkatan kualitas, maka didirikan Malcolm Balridge National Quality Award dalam tahun 1987. Salah satu fasilitas yang mendukung kelancaran proses belajar dan mengajar di prodi Teknik Industri UNUGHA adalah kursi kuliah. Kondisi kursi kuliah yang digunakan saat ini adalah kursi dengan rangka dari besi kotak, alas duduk dari busa, sandaran punggung dari busa serta alas menulis dari kayu. Dari hasil penelitian, diperoleh kegagalan yang paling sering terjadi pada kursi tidak stabil dengan jumlah kerusakan 30 unit dan permasalahan dalam pembuatan kursi kuliah adalah kursi kuliah tidak stabil. Membuat kursi tidak siku, dalam masalah kegagalan produk kursi kuliah ini terjadi dalam proses pemasangan, dan orang yang bertanggung jawab adalah operator karena kurang telitinya saat pemasangan kursi kuliah sehingga kursi tidak siku membuat kursi tidak stabil. Solusinya operator harus lebih teliti dan menggunakan penggaris siku pada saat pemasangan kursi.
Rancang Bangun Nanofarm sebagai Potensi Bertanam Praktis
Authors: Putranto Atikah Nur , Setiowati Amantun Nisa , Putri Monica Achir
Published: March 2022

Nanofarm adalah sebuah alat berbentuk balok yang dapat membantu petani dalam menanam sayuran berusia pendek. Alat ini menawarkan kemudahan bercocok tanam berupa sistem kontrol otomatis sehingga tanpa memerlukan perhatian khusus pun tanaman dapat tumbuh dengan baik. Sistem kontrol yang terdapat pada nanofarm adalah fitur pengatur irigasi, kelembapan tanah, suhu udara, dan pencahayaan. Pembuatan desain gambar menggunakan Solidwork serta coding yang dilakukan menggunakan Microsoft Visual Studio 2016. Pengambilan data dilakukan untuk mengetahui parameter-parameter yang sesuai dengan kondisi tanaman yang akan ditanam dalam alat berbentuk balok dengan ukuran 50×45×70 cm. Nanofarm mampu mendukung urban agriculture dan pertanian presisi dalam masyarakat luas khususnya masyarakat perkotaan dengan lahan terbatas.
CovidXplus-A New Mobile Application for Image-Guided Diagnosis of COVID-19 Patients
Authors: Karar Mohamed Esmail , Ahmad Bilal
Published: March 2022

Patients with unexplained pneumonia were discovered in Wuhan City, China, at the end of 2019, according to the World Health Organization (WHO). Chinese authorities announced on January 2020 that they discovered a new virus that causes these infections. That's why the virus was assigned the name of novel Coronavirus Disease 2019 (COVID-19). It is a new disease that affects the lungs and airways and can cause mild to severe illness, as well as pneumonia. Coronaviruses are a wide family of viruses that can infect both animals and humans. Extreme Acute Respiratory Syndrome 2 is the most recent Coronavirus to be discovered (SARS-CoV-2). According to the results, the virus spreads from person to person in close contact over a distance of about 2 meters. When someone coughs or sneezes, respiratory droplets are released, which spread the virus. As a result, we need to build an application that allows use of computer-aided diagnosis (CAD) systems for detection of the COVID-19 based on radiological techniques. This paper proposes a novel mobile application based on fine-tuned transfer learning models to boost the efficiency of CAD systems in the detection of the highly suspected COVID-19 patients using medical X-ray images. Three fine-tuned deep learning models, namely ResNet50, ResNet101, and ResNet152 are exploited in this study.
In vitro and In silico Studies on Leonotis nepetifolia (L.) R. Br. Root Extract against Cancer Cells
Authors: Merecz-Sadowska Anna , Sitarek Przemysław , Śliwiński Tomasz , Zajdel Karolina , Malinowska Katarzyna , Zielińska-Bliźniewska Hanna , Kucharska Ewa , Zajdel Radosław
Published: March 2022

Background:

Leonotis nepetifolia (L.) R. Br. (Lamiaceae) is a shrub traditionally used to alleviate inflammatory conditions.

Objective:

The present study aimed at investigating the biological activity of methanolic nontrans-formed and transformed Rhizobium rhizogenes root extracts from L. nepetifolia against human melanoma cells.

Methods:

Cytotoxicity and genotoxicity properties, the impact on topoisomerase I activity, and proapoptotic activity were evaluated by the MTT test, comet assay, topoisomerase I assay, and fluorescence-activated cell sorting analysis. Moreover, the expressions of p53 were examined by qPCR and Western blot analysis. Docking studies were conducted to assess the potential interac-tions of the identified phytochemicals with the p53 binding protein Mdm-2, and computational analyses exhibited their antioxidant potential.

Results:

Both extracts showed cytotoxic potential against human melanoma cells, but generally the activity was more potent for transformed roots than untransformed (IC50 760 μg/mL and 980 μg/mL, respectively). A similar effect was revealed during the evaluation of genotoxic and proapoptotic properties. Moreover, the expression of p53 was also found to be increased after ex-tract treatment. The most dominant identified compounds in both extracts were as follows: (+)-catechin, p-coumaric acid, m-coumaric acid, and (+)-rosmarinic acid. Docking studies and compu-tational analysis showed that (+)-rosmarinic acid possesses the highest binding affinity to the p53 binding protein, Mdm-2, and exhibits the best antioxidant property from the most commonly iden-tified phytochemicals.

Conclusion:

Our findings revealed the potential of L. nepetifolia transformed root extract as a source of bioactive compounds with cytotoxic, genotoxic, and proapoptotic activity against human melanoma cells as well as antioxidant properties.
WITHDRAWN: Therapeutic Targeted Approaches for Covid-19 Treatment
Authors: Rajawat Jyotika , Misra Gauri , Anvikar Anup K , Era Benedetta
Published: March 2022

Since the authors are not responding to the editor’s requests to fulfill the editorial requirement, therefore, the article has been
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Nicotinamide Adenine Dinucleotide in the Development and Treatment of Cardiac Remodeling and Aging
Authors: Wang Fang , Pei Zuowei , Wang Kanglin , Wang Lei
Published: March 2022

Background:

Recently, the beneficial effects of nicotinamide adenine dinucleotide (NAD+) as an antiaging and antioxidant molecule have become a focus of research. However, the mechanisms by which NAD+ supplementation affects the associated metabolites under physiological conditions remain unclear. Specifically, although NAD+ is involved in several processes that are dysregulated in cardiovascular diseases, some effects of NAD+ precursors and NAD+ on cardiac diseases have started to gain recognition only recently.

Objective:

To discuss the influence of NAD+ supplementation on adverse cardiac remodeling and aging.

Results:

Supplementation with NAD+ precursors or nicotinamide riboside, which enhances or supplements the NAD+ metabolome, might have a protective effect on the heart. NAD+ can alleviate chronic heart failure via a mitochondrial oxidation–reduction (redox) state mechanism. Furthermore, NAD+ replenishment can improve the life span of mice.

Conclusion:

NAD+ exerts considerable antiaging and antioxidant effects with promising therapeutic effects. However, its effect in humans and use as a dietary supplement need to be studied further.
Novel Compound, ND-17, Regulates the JAK/STAT, PI3K/AKT, and MAPK Pathways and Restrains Human T-lymphoid Leukemia Development
Authors: Ma Weina , Liu Yanhong , Lei Panpan , Zhu Man , Pan Xiaoyan
Published: March 2022

Background:

T cell acute lymphoblastic leukemia (T-ALL) is an invasive hematological
malignant disorder of T cell progenitors. The Janus kinase-signal transducer and activator of transcription
(JAK-STAT) signaling pathway plays an important role in the development of T-ALL and in the
inhibition of the key molecule, JAK2, and could suppress T-ALL cell proliferation.

Objective:

The objective of this study was to investigate the in vitro anti-tumor effects of a novel nilotinib
derivative, ND-17, on cancer cell lines via its interactions with JAK2.

Methods:

The effects of ND-17 on cell proliferation and on cell cycle and apoptosis were evaluated
using the tetrazolium assay and flow cytometry, respectively. In addition, the ND-17/JAK2 binding
interactions were evaluated using surface plasmon resonance and western blot analyses.

Results:

ND-17 exerted the greatest inhibitory effects on T-ALL cells amongst all hematological cancer
cell lines tested. Flow cytometric analysis indicated that ND-17 blocked the cell cycle at the S
phase in T-ALL cells. Nilotinib did not significantly inhibit T-ALL cell growth or regulate the cell
cycle. Preliminary investigations revealed that the regulation of cyclin-dependent kinases/cyclins was
attributed to ND-17-induced cell cycle arrest. Furthermore, ND-17 could bind to JAK2 with strong
affinity, and more importantly, ND-17 bound to the ATP pocket of JAK2 in a manner similar to the
potent inhibitor. Thus, ND-17 treatment exhibited a prominent effect in inhibiting the phosphorylation
of JAK2 in T-ALL cells. An increase in the phosphorylation of JAK2 was observed in interleukin-6-
stimulated Jurkat cells, which was reversed by ND-17 treatment. Meanwhile, the combination of TG-
101348 and ND-17 led to further improvement in inhibiting the phosphorylation of JAK2. Moreover,
the transfection and knockdown of JAK2 altered the inhibitory effect of ND-17 on Jurkat cell viability.
In addition, ND-17 treatment suppressed the JAK/STAT, phosphatidylinositol-3-kinase/protein kinase
B/mechanistic target of rapamycin, and mitogen-activated protein kinase/extracellular signal-regulated
protein kinases 1 and 2 signaling pathways.

Conclusion:

These findings suggest that ND-17 could be a promising JAK2 inhibitor for the treatment
of T-ALL.
Electrochemical Cell-based Biosensors for Biomedical Applications
Authors: Özsoylu Dua , Wagner Torsten , Schöning Michael Josef
Published: March 2022

Electrochemical cell-based biosensors have attracted increasing interest within the last 15 years, with a large number of reports generally dealing with the sensors’ sensitivity, selectivity, sta-bility, signal-to-noise ratio, spatiotemporal resolution, etc. However, only a few of them are now available as commercial products. In this review, technological advances, current challenges, and opportunities of electrochemical cell-based biosensors are presented. The article encompasses emerging studies on cell-based biological field-effect devices, cell-based impedimetric sensors, and cell-based microelectrode arrays, mainly focusing on the last five years (from 2016 to mid-2021). In addition, special attention lies in recent progress at the single-cellular level, including intracellular monitoring with high spatiotemporal resolution as well as integration into microfluidics for lab-on-a-chip applications. Moreover, a comprehensive discussion on challenges and future perspectives will address the future potential of electrochemical cell-based biosensors.
Rapid Analytical Method Development and Validation for the Simultaneous
Estimation of 5-Fluorouracil and Cannabidiol in Plasma and Lipid-based
Nanoformulations
Authors: Hasan Nazeer , Imran Mohammad , Jain Dhara , Shamim Athar , Beg Sarwar , Kesharwani Prashant , Jain Gaurav , Ahmad Farhan J.
Published: March 2022

Background:

5-Fluorouracil (5-FU) is a well-established anticancer drug. Several studies have also demonstrated the anticancer potential of Cannabidiol (CBD) against various malignancies, including skin cancer. Reported synergistic effects of this combination fascinate researchers to consider this for the management of skin cancer.

Methods:

A simple and robust HPLC method for simultaneous estimation of 5-FU and CBD at its single wavelength (237 nm) was developed and validated. The separation of these compounds was performed on Waters® HPLC system with Hypersil™ C18 RP-column using methanol and water in gradient flow as mobile phase. The method could effectively quantify the nanogram levels of both analytes simultaneously in plasma spiked samples and various nanoformulations. The analytical performance of the proposed method was validated in terms of various parameters, such as linearity, ruggedness, specificity, and few others.

Results:

5-FU as well as CBD were successfully detected at 237 nm with retention time 1.4 and 1.84 minutes respectively. Calibration curves were found to be linear with R2 values of 0.985 and 0.984 for 5-FU and CBD respectively. The method was linear, precise, specific and robust. Additionally, prepared method successfully employed in determining concentration of both drugs in combitorial nanoformulations.

Conclusion:

The findings show that the developed method was simple, reliable, sensitive and economical. It could be employed for the simultaneous estimation of 5-FU and CBD in various in vitro and in vivo studies.
The Potential Role of the Serotonin Transporter as a Drug Target
against Parasitic Infections: A Scoping Review of the Literature
Authors: Montazeri Mahbobeh , Fakhar Mahdi , Keighobadi Masoud
Published: March 2022

Background:

Several in vitro and in vivo biological activities of serotonin, 5-
hydroxytryptamine (5-HT), as a bioactive molecule, and its transporter (5-HT-Tr) were
evaluated in parasitic infections.

Objective:

Herein, the roles of 5-HT and 5-HTR in helminths and protozoan infections with
medical and veterinary importance are reviewed.

Methods:

We searched information in 4 main databases and reviewed published literature
about the serotonin transporter's role as a promising therapeutic target against pathogenic
parasitic infections between 2000 and 2021.

Results:

Based on recent investigations, 5-HT and 5-HT-Tr play various roles in parasite
infections, including biological function, metabolic activity, organism motility, parasite survival,
and immune response modulation. Moreover, some of the 5-HT-TR in Schistosoma
mansoni showed an excess of favorite substrates for biogenic amine 5-HT compared to their
mammalian hosts. Furthermore, the main neuronal protein related to the G protein-coupled
receptor (GPCR) was identified in S. mansoni and Echinococcus granulosus, playing main
roles in these parasites. In addition, 5-HT increased in toxoplasmosis, giardiasis, and Chagas
disease. On the other hand, in Plasmodium spp., different forms of targeted 5-HTR
stimulate Ca2+ release, intracellular inositol triphosphate (ITP), cAMP, and protein kinase A
(PKA) activity.

Conclusion:

This review summarized the several functional roles of the 5-HT and the importance
of the 5-HT-TR as a drug target with minimal harm to the host to fight against
helminths and protozoan infections. Hopefully, this review will shed light on research regarding
serotonin transporter-based therapies as a potential drug target soon.
A Novel Method for Predicting Essential Proteins by Integrating Multidimensional
Biological Attribute Information and Topological Properties
Authors: Lu Hanyu , Shang Chen , Zou Sai , Cheng Lihong , Yang Shikong , Wang Lei
Published: March 2022

Background:

Essential proteins are indispensable to the maintenance of life activities and
play essential roles in the areas of synthetic biology. Identification of essential proteins by computational
methods has become a hot topic in recent years because of its efficiency.

Objective:

Identification of essential proteins is of important significance and practical use in the areas
of synthetic biology, drug targets, and human disease genes.

Method:

In this paper, a method called EOP (Edge clustering coefficient -Orthologous-Protein) is proposed
to infer potential essential proteins by combining Multidimensional Biological Attribute Information
of proteins with Topological Properties of the protein-protein interaction network.

Results:

The simulation results on the yeast protein interaction network show that the number of essential
proteins identified by this method is more than the number identified by the other 12 methods (DC,
IC, EC, SC, BC, CC, NC, LAC, PEC, CoEWC, POEM, DWE). Especially compared with DC (Degree
Centrality), the SN (sensitivity) is 9% higher, when the candidate protein is 1%, the recognition rate is
34% higher, when the candidate protein is 5%, 10%, 15%, 20%, 25% the recognition rate is 36%, 22%,
15%, 11%, 8% higher, respectively.

Conclusion:

Experimental results show that our method can achieve satisfactory prediction results,
which may provide references for future research.
Synthesis and Biological Evaluation of Coumarin Carboxamides as Selective and
Potent Inhibitors of Carbonic Anhydrases IX and XII
Authors: Thacker Pavitra S. , Mohammed Arifuddin , Supuran Claudiu T. , Tiwari Prerna L. , Goud Nerella S. , Srikanth Danaboina , Angeli Andrea
Published: March 2022

Background:

Carbonic anhydrases (CAs, EC 4.2.1.1) catalyze the reversible hydration of carbon dioxide to
bicarbonate and proton. Inhibition of isoforms IX and XII could aid in the amelioration of cancer.

Objective:

A series of coumarin carboxamides (6a-j) were synthesized and were assayed against hCA isoforms I, II,
IX, and XII.

Methods:

Thin Layer Chromatography (TLC) analysis was done by utilizing Merck silica gel 60 F254 aluminum
plates. Stuart Digital Melting Point Apparatus (SMP 30) was used in determining the melting points of the compounds,
which are uncorrected. High Resolution Mass Spectra (HRMS) were determined by Agilent QTOF mass spectrometer
6540 series instrument and were performed using ESI techniques at 70eV.

Result:

All the compounds selectively inhibited isoforms IX and XII as against hCAs I and II. Compounds 6a-e exhibited
the best inhibitory profiles against hCA IX (Ki < 25 nM). The isoform hCA XII was effectively inhibited by all
compounds showing the Ki values less than 65 nM. The Compounds 6a, 6b, 6g, 6h, and 6j exhibited Ki values less
than 10 nM. The binding interactions of the most potent compounds, 6a and 6b, were investigated through docking
studies with hCAs IX and XII.

Conclusion:

These compounds may be utilized as useful starting points for the design and development of selective
and potent hCA IX and XII inhibitors.
Clinical Correlations of Lipid Profiles with the Age and Gender in the Coronary
Artery Disease Patients: A Study of 3878 CAD Patients from India
Authors: Mir Rashid , Elfaki Imadeldin , Frah Ehab A.M. , Alzahrani Khalid J. , Mir Mohammad Muzaffar , Banu Shaheena
Published: March 2022

Background:

Cardiovascular diseases (CVDs) are crucial cause of death and
hospitalization all over the world including India. The CVDs including the coronary artery disease
(CAD) are developed by the interaction of genetic and environmental factors. Hyperlipidemia is a
traditional risk factor for CVD.The aim of this study was to study the clinical corrections of Lipid Profiles with the Age and Gender
in the Coronary Artery Disease Patients:

Methods:

In this study, we have investigated the effect of age and sex on in lipid profile in 3878
(1171 females and 2707 males) CAD patients from India.

Results:

The plasma TG was higher in males than in females regardless of the age. Results showed
that CAD female patients had significantly increased HDL-C than their aged matched males.
Moreover, the plasma TC and LDL-C were significantly higher in males than females until age 40
years. Then after the age of 40 years, TC and LDL-C become significantly higher in females than
in males. In addition, we found that more than 85% of CAD cases were <55 years old, and about
30% of CAD cases had normal lipid profile.

Conclusion:

We conclude that elderly females are at a greater risk for CAD than males. Moreover,
there were no significant differences in CVDs causes between nonelderly and elderly females. In
addition, a higher percentage of cases were premature CAD, and 30% of CAD may be caused by
loci that are not related to lipid metabolism.
Serum Cytokine Levels of Systemic Lupus Erythematosus Patients in the
Presence of Concomitant Cardiovascular Diseases
Authors: Melamud Mark M. , Ermakov Evgeny A. , Boiko Anastasiia S. , Parshukova Daria A. , Sizikov Alexey E. , Ivanova Svetlana A. , Nevinsky Georgy A. , Buneva Valentina N.
Published: March 2022

Background:

Systemic lupus erythematosus (SLE) is known to be associated with an increased risk of cardiovascular diseases (CVD). SLE patients suffer from CVD 3.5 times more often than healthy people. Cytokine-mediated inflammation is actively involved in the development of cardiovascular pathology.

Objective:

Here, we analyzed serum levels of nine cytokines of steroids-treated SLE patients depending on the presence of concomitant CVD.

Methods:

The levels of interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-10, IL-21, tumor necrosis factor α (TNFα), B-cell activating factor (BAFF), and a proliferation-inducing ligand (APRIL) were analyzed using multiplex immunoassay.

Results:

In the total group of SLE patients (n=29), the concentrations of IL-6 and IL-10 were higher, and the APRIL level decreased compared to healthy donors (n=39, p<0.05). The same changes were observed in the group of patients without CVD (n=15); the levels of IL-6 and IL-10 were found to be increased, and the level of APRIL was lower than in healthy individuals (p<0.05). In the group of SLE patients with CVD (n=14), the concentrations of IL-4, IL-6, IL-10, and TNFα were found to be increased (p<0.05). Interestingly, the levels of TNFα and BAFF in SLE patients with CVD were higher than in patients without cardiovascular pathology. Thus, TNFα and BAFF levels were significantly altered in SLE with concomitant CVD compared to SLE without CVD.

Conclusion:

These findings suggest that cytokine profiles in SLE with concomitant CVD and SLE without CVD are different, which should be considered in further research with large samples.
Serum Klotho Concentrations in Young and Older Men During Prolonged
Exercise in Temperate and Hot Conditions
Authors: King Kelli E. , McCormick James J. , Notley Sean R. , Fujii Naoto , Kenny Glen P.
Published: March 2022

Background:

The protein klotho protects cellular function during various physiological
stressors, such as exercise, however it is unknown how the age-related decline in klotho production
affects responses during exercise, especially in the heat.

Objective:

Our objective was to determine the effect of exercise in temperate and hot environmental
conditions on serum klotho concentrations in young and older men.

Methods:

12 young (mean ± SD: 22 ± 3 years) and 12 older (59 ± 4 years) men performed 180
minutes of moderate-intensity treadmill walking (metabolic rate: 200 W/m2) in a temperate (wetbulb
globe temperature [WBGT] 16°C, achieved with 21.9°C, 35% relative humidity [RH]) and hot
(WBGT 32°C, achieved with 41.4°C, 35% RH) environment. Serum klotho was assessed before
and after exercise, as well as 60-min post-exercise recovery in the respective environments.

Results:

Absolute klotho concentrations were greater in young versus older men during all measured
time points in the temperate (p = 0.032), but not the hot condition (p = 0.064). In the hot condition,
the change in serum klotho from baseline was significantly higher after exercise in the heat
(mean ± SEM: +251 ± 73 pg/mL) than the temperate (+75 ± 57 pg/mL) environment in both groups
(p = 0.026). However, this elevation was not maintained during recovery.

Conclusion:

We showed that prolonged exercise in a temperate environment does not elicit a
klotho response in either group. In contrast, despite lower resting klotho levels, the older men
showed a similar exercise-induced increase in serum klotho response as their younger counterparts.
A Review on Potential Activities of Different Parts of Cassia sophera as Antidiabetic,
Antimicrobial and Anticancer Agent
Authors: Chakraborty Atyurmila , Acharjya Sasmita Kumari , Tripathy Bimala , Shree Dipthi
Published: March 2022

Background:

Herbal medicines are one of the giant creations of nature. Several medicinal agents developed from natural sources, which open a new era of health sciences. Cassia sophera is a prevalent medicinal plant that is locally known as “Kasondi”, belonging to the family Caesalpinaceae. Many secondary metabolites like alkaloids, tannins, anthraquinones, flavonoids are isolated from roots, stems, leaves, flowers, and seeds of C. sophera, which are very effective against several diseases.

Objective:

Cassia sophera has an enormous potential in public health to protect from diabetes, microbes, and cancer. Hence, the aim of the present review is to collect the reported antidiabetic, antimicrobial, and anticancer activity of Cassia sophera along with a detailed discussion about mechanism of action of var-ious phytoconstituents.

Discussion:

Antidiabetic activity of Cassia sophera is due to the activation of β cell and stimulation of surviving pancreatic β-cells to release more insulin. Plasma membrane disruption, inhibition of cell wall formation, inhibition of cell division by the Cassia sophera is the prime causes of antimicrobial activity. The anticancer activity of the plant is mainly due to the prevention of reactive oxygen species formation, induction of cell cycle arrest, apoptosis, and angiogenesis.

Conclusions:

The findings from this review article confirm the potential activities and mechanisms of various phytoconstituents responsible for preventing diabetes, cancer, and microbial infections. Further investigation regarding possible pharmaceutical side effects, contraindications, drug interactions involv-ing drugs synthesized from C. sophera should be carried out by the researchers.
A Review on Unknown Repercussions Associated with Metallic Nanoparticles and their Rectification Techniques
Authors: Aqeel Saman , Gupta Aparna , Singh Lalit
Published: March 2022

Background:

The wide use of metallic nanoparticles (MNPs) has toxic effects on the human body affecting vital organs such as brain, liver and kidney. Therefore it is necessary to develop approaches to eradicate such health issues without compromising plus the potential benefits of the respective metallic nanoparticles including silver, gold, zinc, copper, etc.

Objective:

This study aimed to assess methods which can mutually reduce the nanotoxicity while retaining the therapeutic benefits of metal-based nanocarriers.

Methods:

The implementation of certain methods, such as the addition of chelating agents, providing protective coatings and surface modification during the synthesis of metallic nanoparticles can subsequently minimize metallic toxicity.

Results:

Through extensive and exhaustive literature survey it was proved that the above strategies are effective in reducing nanotoxic effects which can be further assessed by toxicity assessment tools as biochemistry, histopathology, etc.

Conclusion:

Metallic nanoparticles have emerged as a beneficial tool for treating various diseases such as cancer, hepatitis, etc. Scientists are also preserving their efficacy by escorting novel techniques for limiting its toxicity in the world of nanotechnology.
Role of Cytokines in Chemotherapy-related Cognitive Impairment of
Breast Cancer Patients: A Systematic Review
Authors: Khan Mohd. Ashif , Tyagi Kanika , Masoom Md. , Majid Haya , Garg Aakriti , Bhurani Dinesh , Agarwal Nidhi B.
Published: March 2022

Background:

Cognitive impairment is one of the most common problems experienced by
patients receiving chemotherapy, and evidence suggests that cytokines might play an important role.
Various studies were conducted to evaluate the role of cytokines in chemotherapy-related cognitive
impairment (CRCI). However, the association between CRCI due to cytokines is not well-established.
Thus, this systematic review aims to assess the role of cytokines in CRCI in breast cancer patients.

Methods:

This systematic review was conducted according to the Preferred Reporting Item for Systematic
Review and Meta-analysis (PRISMA) guidelines. An intense literature search was carried out
for inclusion criteria in major databases, including PubMed and Clinicaltrials.gov, in August 2021.
Studies assessing cognitive parameters through objective and subjective assessment in breast cancer
patients receiving chemotherapy were included.

Results:

A total of 4052 studies were identified, and 15 studies were included in this systematic review.
We found that IL-6, IL-1β, and TNF-α were associated with varying degrees of cognitive impairment
in breast cancer patients receiving chemotherapy.

Conclusion:

This systematic review showed a correlation between various cytokines and chemotherapy-
associated cognitive decline in breast cancer patients.
Penguatan pendidikan karakter model social problem solving bagi siswa sekolah dasar
Authors: Wahjusaputri Sintha , Bunyamin Bunyamin , Nastiti Tashia Indah
Published: March 2022

The purpose of community service activities to know how to strengthen the character education model for elementary school students of Muhammadiyah 11 Tanjung Lengkong Jakarta and its application. Research and Development (R D) used for the research method, there are consist of four step: (1) the preliminary step is the firs activity and collecting student information; (2) The planning stage of character education model for students as the development of initial product form; (3) Trial, evaluation and revision phases, and (4) Implementation step of character education. To measure the successful implementation of character education using the Delphi technique. Based on expert judgment, the strengthening of character education model of SD Muhammadiyah 11 Tanjung Lengkong Jakarta can be measured using the Social Problem Solving model. The result of the strengthening of character education model was found on the social skills enhancement of elementary school students of Muhammadiyah 11 Tanjung Lengkong with an average score of 3.75. Therefore the use of Model Social Problem Solving is worth using.
Management of classical Islamic education
Authors: Karisma Leni Martha , Sutarto Joko , Suminar Tri
Published: March 2022

The purpose of this research is to describe the planning, organizing, implementing, and evaluating of classical Islamic education at Kuttab Al Fatih Semarang. This research uses a qualitative approach. Data collection with observation methods, documentation studies and interviews. The validity of data with extended entry techniques, observational diligence techniques, and triangulation techniques. The results showed the management of classical Islamic education in Kuttab Al Fatih Semarang ranging from planning, organizing, implementation, and evaluation done in accordance with the concept of Islam in the heyday of Islam that aspires to build Quality generation. The management of the classical Islamic education in Kuttab Al Fatih is very effective to create students who want to have a character of faith, to be the memorization of the Qur'an, deepen the miracle of the Qur'an, mastering the language of civilization and have the skills of life.
Learning content versus social environment. A comparative analysis from traditional initiation and modern high-schooling in Java (Indonesia)
Authors: De Grave Jean-Marc , Fauziah Puji Yanti
Published: March 2022

The circulation of contents depends on the marked separation of social domains and sub-domains: they are transformed from one domain to another (from the scientific milieu to the media or the school environment, for example). A competition of domains is thus developed, which aims to enhance the radiance of contents reflecting knowledge some of which dominate, others not, others are ignored or unrecognised and still others disappear. Finally, the description of Indonesian high schools presents modes of transmission that do not overvalue the learning content to the detriment of relationship established between the different members of some of these high schools. The two first high schools I have described (SMA3 and de Britto), present in their internal operating mode of transmission the same configuration as the nyantri system found in traditional kanuragan insofar as it shows how it is very difficult, if not impossible, to separate a so-called learning content from a learning community and a social environment. From these two examples we can understand that socialising ability is all the same educable and transmissible in a high formal context, and that the positive integration of the relation to time, space and others clearly adds that of the relation to knowledge.
Penguatan “Kaulinan Barudak Sunda” sebagai permainan tradisional
Authors: Dermawan Windy , Purnama Chandra , Mahyudin Emil
Published: March 2022

This article aimed to strengthen “Kaulinan Barudak Sunda” as a traditional game towards the community in an effort to improved cultural security in Jatinangor District. To achieved this goal, we provided an understanding of the importance of “Kaulinan Barudak” traditional games towards the community through Focus Group Discussion regarding the identification and formulation of activity objectives, socialization, mentoring and practised of Kaulinan Barudak Sunda for children, and evaluation of activities. The method of implementing this activity was Problem Based Learning by solving problems that occurred regarding the fading of knowledge and practised of Kaulinan Barudak Sunda among children, also carried out with the Community Based Research method by involving the community in compiling shared ideas as needed. Based on the resulted of data analysis, we concluded that there was an increase of 68.5% in the aspects of the participants' perceptions, attitudes, and actions regarding traditional games. Thus, this activity contributed to providing knowledge, attitudes, and actions of participants in supporting the strengthening of Kaulinan Barudak Sunda in the Jatinangor District. AbstrakArtikel ini bertujuan memberikan penguatan Kaulinan Barudak Sunda sebagai permainan tradisional terhadap masyarakat sehingga dapat meningkatkan keamanan kultural di Kecamatan Jatinangor. Untuk mencapai tujuan tersebut, kami memberikan pemahaman mengenai pentingnya permainan tradisional Kaulinan Barudak Sunda terhadap masyarakat melalui kegiatan diskusi kelompok terfokus (Focuss Group Discussion) mengenai identifikasi dan perumusan tujuan kegiatan, sosialisasi kegiatan, pendampingan dan praktek beberapa bentuk Kaulinan Barudak Sunda kepada anak-anak, dan evaluasi kegiatan. Metode pelaksanaan kegiatan ini adalah Problem Based Learning dengan memecahkan permasalahan yang terjadi mengenai lunturnya pengetahuan dan praktek Kaulinan Barudak Sunda di kalangan anak-anak, juga dilakukan dengan metode Community Based Research dengan melibatkan masyarakat dalam menyusun gagasan bersama yang diperlukan. Berdasarkan hasil analisis data, kami menyimpulkan bahwa terdapat peningkatan sebesar 68,5% pada aspek persepsi, sikap dan tindakan peserta mengenai permainan tradisional. Dengan demikian, kegiatan ini berkontribusi memberikan pengetahuan, sikap dan tindakan peserta di dalam mendukung penguatan Kaulinan Barudak Sunda sebagai permainan tradisional di Kecamatan Jatinangor.
Assessing stability motor skills of five-to-six-year old and six-to-seven-year old obese children
Authors: Oktavianingsih Eka , Ismiatun Asih Nur
Published: March 2022

This study aims to determine the differences in the stability of motor skills between five-to-six-year old and six-to-seven-year old obese children. A total of 30 obese children were involved as the participants including 15 children aged 5-6 years old and 15 children aged 6-7 years old. Paediatric Balance Scale (PBS) was used to measure the stability motor performance of all participants. The method of data analysis was by means of independent sample T-test with a P-value of 0.05 as the criterion for all statistical significance. The findings of this study revealed that there is a significant difference in stability motor skills in the parameter walking in a bearn, standing with one foot, and axial movement between five-to-six-year old and six-to-seven-year old obese children. Obese children aged 6-7 years old performed better stability motor skills compared to those aged 5-6 years old.
Drawing method with crayons as a medium of expression and imagination for children
Authors: Ariani Ariani , Werdina Woro Asty , Nugrahadi Gihon , Soeriaatmadja Rully
Published: March 2022

Drawing is a medium of expression and a part of children’s creative and imaginative process.  Children can create, share ideas, visualize and realize their imagination through drawing. Drawing with crayons is familiar to children but this does not apply to the children of age between 5 – 12 years old who live in Karawaci district, Indonesia. These children did not have the opportunity to learn additional skills through informal education due to their families’ financial constraint. The purpose of this activity is to provide training in the right drawing methods and to train children to be creative in dealing with the limitations of the financial conditions. A qualitative method with a case study approach is used to achieve the objectives and determine the answers to the problems encountered in this research. Out of the five methods introduced, the blocking and mixing methods were the most preferred techniques by the children because it was easy to use and produced drawing with bright colours which represent their expression and imagination.