bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of GMAW (Gas Metal Arc Welding) Penetration Using Ultrasonics
Authors: Zhang Lu , Okudan Gorkem , Basantes-Defaz Alexandra-Del-Carmen , Gneiting Ryan M. , Subramaniam Sankaran , Ozevin Didem , Indacochea Ernesto
Published: May 2020

Welding defects such as lack of penetration, undercutting, crater crack, burn-through and porosity can occur during manufacturing. Assessing weld quality using nondestructive evaluation methods is important for the quality assurance of welded parts. In this paper, the measurement of weld penetration, which is directly related to weld integrity, is investigated by means of ultrasonics. Both linear and nonlinear ultrasonic methods are studied to assess their sensitivities to weld penetration. Welded plates with different penetration depths controlled by changing weld heat input are manufactured using gas metal arc welding (GMAW). Microscopic properties are assessed after the ultrasonic measurements are completed. Numerical models are built using the weld profile obtained from macrographs to explain the relationship between linear ultrasonic and weld penetration. A quantitative correlation between weld morphology (shape, width and depth) and the energy of linear ultrasonic signal is determined, where the increase of weld bead penetration exceeding the plate thickness results in decrease of the energy of the ultrasonic signal. Minimum detectable weld morphology using linear ultrasonics is defined depending on the selected frequency. Microhardness measurement is conducted to explain the sensitivity of nonlinear ultrasonics to both weld penetration and heterogeneity in weld. The numerical and experimental results show that the weld geometry influences the ultrasonic measurement other than the materials’ properties.
A Windmill-Shaped Molecule with Anthryl Blades to Form Smooth Hole-Transport Layers via a Photoprecursor Approach
Authors: Maeda Akihiro , Nakauchi Aki , Shimizu Yusuke , Terai Kengo , Sugii Shuhei , Hayashi Hironobu , Aratani Naoki , Suzuki Mitsuharu , Yamada Hiroko
Published: May 2020

Preparation of high-performance organic semiconductor devices requires precise control over the active-layer structure. To this end, we are working on the controlled deposition of small-molecule semiconductors through a photoprecursor approach wherein a soluble precursor compound is processed into a thin-film form and then converted to a target semiconductor by light irradiation. This approach can be applied to layer-by-layer solution deposition, enabling the preparation of p–i–n-type photovoltaic active layers by wet processing. However, molecular design principles are yet to be established toward obtaining desirable thin-film morphology via this unconventional method. Herein, we evaluate a new windmill-shaped molecule with anthryl blades, 1,3,5-tris(5-(anthracen-2-yl)thiophen-2-yl)benzene, which is designed to deposit via the photoprecursor approach for use as the p-sublayer in p–i–n-type organic photovoltaic devices (OPVs). The new compound is superior to the corresponding precedent p-sublayer materials in terms of forming smooth and homogeneous films, thereby leading to improved performance of p–i–n OPVs. Overall, this work demonstrates the effectiveness of the windmill-type architecture in preparing high-quality semiconducting thin films through the photoprecursor approach.
The Complex Rayleigh Waves in a Functionally Graded Piezoelectric Half-Space: An Improvement of the Laguerre Polynomial Approach
Authors: Li Ke , Jing Shuangxi , Yu Jiangong , Zhang Xiaoming , Zhang Bo
Published: May 2020

The research on the propagation of surface waves has received considerable attention in order to improve the efficiency and natural life of the surface acoustic wave devices, but the investigation on complex Rayleigh waves in functionally graded piezoelectric material (FGPM) is quite limited. In this paper, an improved Laguerre orthogonal function technique is presented to solve the problem of the complex Rayleigh waves in an FGPM half-space, which can obtain not only the solution of purely real values but also that of purely imaginary and complex values. The three-dimensional dispersion curves are generated in complex space to explore the influence of the gradient coefficients. The displacement amplitude distributions are plotted to investigate the conversion process from complex wave mode to propagating wave mode. Finally, the curves of phase velocity to the ratio of wave loss decrements are illustrated, which offers extra convenience for finding the high phase velocity points where the complex wave loss is near zero.
Well-Posedness and Time Regularity for a System of Modified Korteweg-de Vries-Type Equations in Analytic Gevrey Spaces
Authors: Boukarou Aissa , Guerbati Kaddour , Zennir Khaled , Alodhaibi Sultan , Alkhalaf Salem
Published: May 2020

Studies of modified Korteweg-de Vries-type equations are of considerable mathematical interest due to the importance of their applications in various branches of mechanics and physics. In this article, using trilinear estimate in Bourgain spaces, we show the local well-posedness of the initial value problem associated with a coupled system consisting of modified Korteweg-de Vries equations for given data. Furthermore, we prove that the unique solution belongs to Gevrey space G σ × G σ in x and G 3 σ × G 3 σ in t. This article is a continuation of recent studies reflected.
Minimal Impact One-Dimensional Arrays
Authors: Egghe Leo , Rousseau Ronald
Published: May 2020

In this contribution, we consider the problem of finding the minimal Euclidean distance between a given converging decreasing one-dimensional array X in (R+)∞ and arrays of the form A a = ( a , a , … , a ︸ ,   0 , 0 ,   … a   t i m e s ) , with a being a natural number. We find a complete, if not always unique, solution. Our contribution illustrates how a formalism derived in the context of research evaluation and informetrics can be used to solve a purely mathematical problem.
A Computational Method for Subdivision Depth of Ternary Schemes
Authors: Khan Faheem , Mustafa Ghulam , Shahzad Aamir , Baleanu Dumitru , M. Al-Qurashi Maysaa
Published: May 2020

Subdivision schemes are extensively used in scientific and practical applications to produce continuous shapes in an iterative way. This paper introduces a framework to compute subdivision depths of ternary schemes. We first use subdivision algorithm in terms of convolution to compute the error bounds between two successive polygons produced by refinement procedure of subdivision schemes. Then, a formula for computing bound between the polygon at k-th stage and the limiting polygon is derived. After that, we predict numerically the number of subdivision steps (depths) required for smooth limiting shape based on the demand of user specified error (distance) tolerance. In addition, extensive numerical experiments were carried out to check the numerical outcomes of this new framework. The proposed methods are more efficient than the method proposed by Song et al.
Effect of Thrust on the Structural Vibrations of a Nonuniform Slender Rocket
Authors: Adair Desmond , Nagimova Aigul , Jaeger Martin
Published: May 2020

The vibration characteristics of a nonuniform, flexible and free-flying slender rocket experiencing constant thrust is investigated. The rocket is idealized as a classic nonuniform beam with a constant one-dimensional follower force and with free-free boundary conditions. The equations of motion are derived by applying the extended Hamilton’s principle for non-conservative systems. Natural frequencies and associated mode shapes of the rocket are determined using the relatively efficient and accurate Adomian modified decomposition method (AMDM) with the solutions obtained by solving a set of algebraic equations with only three unknown parameters. The method can easily be extended to obtain approximate solutions to vibration problems for any type of nonuniform beam.
Determination of Referential Rates for Optical Coherence Tomography and Optical Coherence Tomography Angiography Flow Deficits in the Macular Choriocapillaris in Ophthalmologically Healthy Children
Authors: Bakstytė Viktorija , Šniurevičiūtė Liveta , Šimienė Evelina , Skruodytė Justina , Janulevičienė Ingrida
Published: May 2020

Background and Objectives: Despite the growing number of new research publications, normative references for children’s optical coherence tomography (OCT) parameters are still not completed. We chose to explore this topic because of the lack of normative parameters that is due to an improvement in different technologies and instruments. Our aim was to determine referential rates of retinal nerve fiber layer (RNFL) thickness and flow deficits (FD%) in the macular choriocapillaris (CC) in normal eyes of ophthalmologically healthy children. Materials and Methods: Ophthalmologically healthy 8- to 14-year-old individuals participated (n = 75) in this study. OCT images were taken using an swept-source-OCT (SS-OCT) instrument (DRI-OCT Triton, Topcon, Tokyo, Japan). The early treatment diabetic retinopathy study (EDTRS) grid (6 × 6 mm) divided the RNFL into the thickness maps. The FD% values of the CC were calculated on the 3 × 3-mm scans in a 1-mm circle (C1), 1.5-mm rim (R1.5), and the entire 2.5-mm circle (C2.5), and on the 6 x 6-mm scans in a 1-mm circle (C1), 1.5-mm rim (R1.5), the entire 2.5-mm circle (C2.5), 2.5-mm rim (R2.5), and 5-mm circle (C5). Results: Both scan quantifications of FD% in the C1, C2.5, and R1.5 sectors were similar, but the 6 × 6-mm scan measurements were statistically significantly smaller than the 3 × 3-mm ones. Significant moderate correlations were found between axial length (AxL) and FD% in the 6 × 6-mm scans, namely C1 (r = −0.347, p = 0.002), C2.5 (r = −0.337, p = 0.003), R1.5 (r = −0.328, p = 0.004), R2.5 (r = −0.306, p = 0.008), and C5 (r = −0.314, p = 0.006). Conclusions: The thinnest RNFL layers were on the temporal and nasal sides. FD% values in the C1, C2.5, and R1.5 sectors were similar between the 3 × 3-mm and 6 × 6-mm scans. The negative moderate correlations between AxL and FD% were found in all C1, C2.5, C5, R1.5, and R2.5 sectors of the 6 × 6-mm scans. Further prospective studies are needed to determine more accurate normative references for children’s OCT parameters.
Aquaporin-Containing Proteopolymersomes in Polyelectrolyte Multilayer Membranes
Authors: Reurink Dennis M. , Du Fei , Górecki Radosław , Roesink Hendrik D.W. , de Vos Wiebe M.
Published: May 2020

The field of membranes saw huge developments in the last decades with the introduction of both polyelectrolyte multilayer (PEM)-based membranes and biomimetic membranes. In this work, we combine these two promising systems and demonstrate that proteopolymersomes (PP+) with the incorporated aquaporin protein can be distributed in a controlled fashion using PEMs, even on the inner surface of a hollow fiber membrane. In this way, various proteopolymersome multilayers (PPMs) are fabricated using PP+ as the positively charged species in combination with the polyanions poly(styrene 4-sulfonate) (PSS) and poly(acrylic acid) (PAA). It is shown by reflectometry through alternately adsorbing the polyanions and PP+ that, for both PAA and PSS, a good layer growth is possible. However, when the multilayers are imaged by SEM, the PAA-based PPMs show dewetting, whereas vesicular structures can only be clearly observed in and on the PSS-based PPMs. In addition, membrane permeability decreases upon coating the PPMs to 2.6 L∙m−2∙h−1∙bar−1 for PAA/PP+ and 7.7 L∙m−2∙h−1∙bar−1 for PSS/PP+. Salt retentions show that PAA/PP+ layers are defective (salt retentions <10% and high molecular weight cut-off (MWCO)), in line with the observed dewetting behavior, while PPMs based on PSS show 80% MgSO4 retention in combination with a low MWCO. The PSS/PP+ membranes show a Donnan-exclusion behavior with moderate MgCl2 retention (50%–55%) and high Na2SO4 retention (85%–90%) indicating a high amount of negative charge present within the PPMs. The corresponding PEMs, on the other hand, are predominately positively charged with MgCl2 retention of 97%–98% and Na2SO4 retention of 57%–80%. This means that the charge inside the multilayer and, thus, its separation behavior can be changed when PP+ is used instead of a polycation. When comparing the PPM membranes to the literature, similar performances are observed with other biomimetic membranes that are not based on interfacial polymerization, but these are the only ones prepared using a desired hollow fiber geometry. Combining PEMs and biomimetic approaches can, thus, lead to relevant membranes, especially adding to the versatility of both systems.
A Comparison of Photoelastic and Finite Elements Analysis in Internal Connection and Bone Level Dental Implants
Authors: Herráez-Galindo Cristina , Torres-Lagares Daniel , Martínez-González Álvaro-José , Pérez-Velasco Andrea , Torres-Carranza Eusebio , Serrera-Figallo María-Angeles , Gutiérrez-Pérez José-Luis
Published: May 2020

This study is a contribution to our understanding of the mechanical behaviour of dental implants through the use of the finite element and the photoelastic methods. Two internal connection and bone level dental implants with different design have been analysed (M-12 by Oxtein S.L., Zaragoza, Spain, and ASTRA, from Dentsply Sirona, Charlotte, NC, USA), evaluating the stress distribution produced by axial stresses and a comparison has been established between them, as well as between the two methods used, in order to validate the adopted hypotheses and correlate the numerical modelling performed with experimental tests. To load the implant in laboratory testing, a column was placed, such that the loading point was about 9.3 mm from the upper free surface of the resin plate. This column connects the implant with the weights used to define the test load. In turn, support for both plates was achieved by two 6 mm bolts 130 mm apart and located on a parallel line with the resin (flush with the maximum level of the implant), at a depth of 90 mm. The results obtained with both methods used were similar enough. The comparison of results is fundamentally visual, but ensures that, at least in the range of forces used, both methods are similar. Therefore, the photoelastic method can be used to confirm in a real way the virtual conditions of the finite element models, with the implications in the investigation of dental implants that this entails.
Microstructural Evolution and Wear Behavior of SiCp/7085 Composites Manufactured by Ultrasonic Stirring Casting
Authors: Li Zhenghua , Jiang Ripeng , Li Xiaoqian , Jia Xiang , Zhang Lihua
Published: May 2020

In this work, SiCp/7085 composites with a volume fraction of 10% were fabricated by ultrasonic stirring casting. The evolution of the microstructure and wear properties of SiCp/7085 composites, as well as the mechanism of ultrasonic action in the process of preparation, when the ultrasonic treatments last for 0 min, 5 min, 10 min, and 15 min, respectively, were studied using the optical effects (OM), scanning electron microscope (SEM), and X-ray diffraction (XRD). The experimental results show that the high temperature and high pressure formed by the cavitation of ultrasound can effectively eliminate the cluster of particles, improve the distribution of SiC particles, increase the interfacial wettability of the composites, and form MgAl2O4 and MgO on the particle surface, thereby ultimately improving the wear resistance of the composites. Because the particles in the melt are affected by the segregation effect of ultrasonic standing wave field, there exists an optimal value for the ultrasonic treatment time. When the melt is treated with the ultrasonic wave with an amplitude of 12 μm and a treatment duration of 10 min in the test conditions given in this paper, the composites have the most uniform distribution of particles, the best wear resistance, a large and stable friction coefficient, and the minimum weight loss of sample wear: 12 mg. The change of wear resistance is consistent with the variation of solidification structure and interface properties.
Patterning of Particles and Live Cells at Single Cell Resolution
Authors: Hacohen Adar , Jessel Hadass R. , Richter-Levin Alon , Shefi Orit
Published: May 2020

The ability to manipulate and selectively position cells into patterns or distinct microenvironments is an important component of many single cell experimental methods and biological engineering applications. Although a variety of particles and cell patterning methods have been demonstrated, most of them deal with the patterning of cell populations, and are either not suitable or difficult to implement for the patterning of single cells. Here, we describe a bottom-up strategy for the micropatterning of cells and cell-sized particles. We have configured a micromanipulator system, in which a pneumatic microinjector is coupled to a holding pipette capable of physically isolating single particles and cells from different types, and positioning them with high accuracy in a predefined position, with a resolution smaller than 10 µm. Complementary DNA sequences were used to stabilize and hold the patterns together. The system is accurate, flexible, and easy-to-use, and can be automated for larger-scale tasks. Importantly, it maintains the viability of live cells. We provide quantitative measurements of the process and offer a file format for such assemblies.
Toward a Symbiotic Perspective on Public Health: Recognizing the Ambivalence of Microbes in the Anthropocene
Authors: Sariola Salla , Gilbert Scott F.
Published: May 2020

Microbes evolve in complex environments that are often fashioned, in part, by human desires. In a global perspective, public health has played major roles in structuring how microbes are perceived, cultivated, and destroyed. The germ theory of disease cast microbes as enemies of the body and the body politic. Antibiotics have altered microbial development by providing stringent natural selection on bacterial species, and this has led to the formation of antibiotic-resistant bacterial strains. Public health perspectives such as “Precision Public Health” and “One Health” have recently been proposed to further manage microbial populations. However, neither of these take into account the symbiotic relationships that exist between bacterial species and between bacteria, viruses, and their eukaryotic hosts. We propose a perspective on public health that recognizes microbial evolution through symbiotic associations (the hologenome theory) and through lateral gene transfer. This perspective has the advantage of including both the pathogenic and beneficial interactions of humans with bacteria, as well as combining the outlook of the “One Health” model with the genomic methodologies utilized in the “Precision Public Health” model. In the Anthropocene, the conditions for microbial evolution have been altered by human interventions, and public health initiatives must recognize both the beneficial (indeed, necessary) interactions of microbes with their hosts as well as their pathogenic interactions.
Microbial Safety of Milk Production and Fermented Dairy Products in Africa
Authors: Owusu-Kwarteng James , Akabanda Fortune , Agyei Dominic , Jespersen Lene
Published: May 2020

In Africa, milk production, processing and consumption are integral part of traditional food supply, with dairy products being a staple component of recommended healthy diets. This review provides an overview of the microbial safety characteristics of milk production and fermented dairy products in Africa. The object is to highlight the main microbial food safety hazards in the dairy chain and to propose appropriate preventive and control measures. Pathogens of public health concern including Mycobacterium bovis, Brucella abortus and Coxiella burnettii, which have largely been eradicated in many developed nations, still persist in the dairy chain in Africa. Factors such as the natural antimicrobial systems in milk and traditional processing technologies, including fermentation, heating and use of antimicrobial additives, that can potentially contribute to microbial safety of milk and dairy products in Africa will be discussed. Practical approaches to controlling safety hazards in the dairy chain in Africa have been proposed. Governmental regulatory bodies need to set the necessary national and regional safety standards, perform inspections and put measures in place to ensure that the standards are met, including strong enforcement programs within smallholder dairy chains. Dairy chain actors would require upgraded knowledge and training in preventive approaches such as good agricultural practices (GAP), hazard analysis and critical control points (HACCP) design and implementation and good hygienic practices (GHPs). Food safety education programs should be incorporated into school curricula, beginning at the basic school levels, to improve food safety cognition among students and promote life-long safe food handling behaviour.
Efficient Genome Editing in Bacillus licheniformis Mediated by a Conditional CRISPR/Cas9 System
Authors: Li Youran , Wang Hanrong , Zhang Liang , Ding Zhongyang , Xu Sha , Gu Zhenghua , Shi Guiyang
Published: May 2020

Bacillus licheniformis is widely used to produce multiple enzymes and chemicals in industrial fermentation. It is also an organism that is hard to genetically manipulate, which is mainly attributed to its extremely low transformation efficiency. The lack of genetic modification technology severely limits its further application. In this study, an all-in-one conditional clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 plasmid was developed for B. licheniformis with the cas9 gene under the control of a xylose-inducible promoter. By means of this design, the expression of the cas9 gene could be repressed without xylose, which significantly improved the transformation ratio from less than 0.1 cfu/μg to 2.42 cfu/μg DNA. Compared with this conditional system, a constitutive overexpression system led to significant growth retardation in bacterial cells. Both the biomass and specific growth rate decreased greatly. After transformation, successful genome editing could be triggered by 0.5% xylose. When the α-amylase gene amyL was used as a genomic target, the efficiencies of its disruption using three different protospacer-adjacent motif (PAM) sequences were 64.3%, 70.9%, and 47.1%, respectively. Moreover, temperature plays a pivotal role in the function of the constructed CRISPR system. The maximum success rate reached 97% at 20 °C, while higher temperatures negatively impacted the function of the system. These results suggested that the design with a cas9 gene under the strict control of a xylose-inducible promoter significantly improved the success rate of genome editing in this host. This work contributes to the development of genetic manipulation and furthers the use of B. licheniformis as an efficient industrial workhorse.
Heavy Minerals as Indicators of the Source and Stratigraphic Position of the Loess-Like Deposits in the Orava Basin (Polish Western Carpathians)
Authors: Chmielowska Dorota , Salata Dorota
Published: May 2020

This study is focused on the loess-like deposits accumulated on glaciofluvial fans of the Czarny Dunajec River in the Orava Basin (Southern Poland). The deposition of these sediments took place during three cold intervals of the Pleistocene: Würm, Riss, and Günz/Mindel. So far, the provenance and age of the deposits has not been precisely defined, even though the development of each fan is believed to be related to the successive glacial periods in the Tatra Mountains. Heavy minerals were studied to determine the source of the deposits. Heavy mineral analyses revealed that zircon, tourmaline, rutile, garnet, amphibole, epidote, and apatite are the typical constituents of the heavy mineral fraction. Abundances of heavy minerals differ in each of the Pleistocene fans of the Czarny Dunajec River, especially the amphibole content. However, the chemical composition of garnet, amphibole, and tourmaline is rather uniform. This research showed that mainly medium-grade metamorphic rocks with a subordinate share of high-grade metamorphics, and granitic rocks are the dominant source rocks of the deposits studied. Such rocks are exposed in the Western Tatra Mountains, which most probably supplied the Orava Basin with clastic material. Change in abundances of heavy minerals in the succession may reflect the progressive erosion of the source area. Grain-size distribution and textural features of the sampled sediments suggest fluvial and aeolian modes of transportation. Additionally, this study indicated that heavy minerals may be used to correlate the loess covers in the Orava Basin.
A Comprehensive Review of Rare Earth Elements Recovery from Coal-Related Materials
Authors: Zhang Wencai , Noble Aaron , Yang Xinbo , Honaker Rick
Published: May 2020

Many studies have been published in recent years focusing on the recovery of rare earth elements (REEs) from coal-related materials, including coal, coal refuse, coal mine drainage, and coal combustion byproducts particularly fly ash. The scientific basis and technology development have been supported by coal geologists and extractive metallurgists, and through these efforts, the concept has progressed from feasibility assessment to pilot-scale production over the last five years. Physical beneficiation, acid leaching, ion-exchange leaching, bio-leaching, thermal treatment, alkali treatment, solvent extraction, and other recovery technologies have been evaluated with varying degrees of success depending on the feedstock properties. In general, physical beneficiation can be a suitable low-cost option for preliminary upgrading; however, most studies showed exceedingly low recovery values unless ultrafine grinding was first performed. This finding is largely attributed to the combination of small RE-bearing mineral particle size and complex REE mineralogy in coal-based resources. Alternatively, direct chemical extraction by acid was able to produce moderate recovery values, and the inclusion of leaching additives, alkaline pretreatment, and/or thermal pretreatment considerably improved the process performance. The studies reviewed in this article revealed two major pilot plants where these processes have been successfully deployed along with suitable solution purification technologies to continuously produce high-grade mixed rare earth products (as high as +95%) from coal-based resources. This article presents a systematic review of the recovery methods, testing outcomes, and separation mechanisms that are involved in REE extraction from coal-related materials. The most recent findings regarding the modes of occurrence of REEs in coal-related materials are also included.
The Geraniin-Rich Extract from Reunion Island Endemic Medicinal Plant Phyllanthus phillyreifolius Inhibits Zika and Dengue Virus Infection at Non-Toxic Effect Doses in Zebrafish
Authors: Haddad Juliano G. , Grauzdytė Dovilė , Koishi Andrea Cristine , Viranaicken Wildriss , Venskutonis Petras Rimantas , Nunes Duarte dos Santos Claudia , Desprès Philippe , Diotel Nicolas , El Kalamouni Chaker
Published: May 2020

The mosquito-borne viruses dengue (DENV) and Zika (ZIKV) viruses are two medically important pathogens in tropical and subtropical regions of the world. There is an urgent need of therapeutics against DENV and ZIKV, and medicinal plants are considered as a promising source of antiviral bioactive metabolites. In the present study, we evaluated the ability of Phyllanthus phillyreifolius, an endemic medicinal plant from Reunion Island, to prevent DENV and ZIKV infection in human cells. At non-cytotoxic concentration in vitro, incubation of infected A549 cells with a P. phillyreifolius extract or its major active phytochemical geraniin resulted in a dramatic reduction of virus progeny production for ZIKV as well as four serotypes of DENV. Virological assays showed that P. phillyreifolius extract-mediated virus inhibition relates to a blockade in internalization of virus particles into the host cell. Infectivity studies on ZIKV showed that both P. phillyreifolius and geraniin cause a loss of infectivity of the viral particles. Using a zebrafish model, we demonstrated that administration of P. phillyreifolius and geraniin has no effect on zebrafish locomotor activity while no morbidity nor mortality was observed up to 5 days post-inoculation. Thus, P. phillyreifolius could act as an important source of plant metabolite geraniin which is a promising antiviral compound in the fight against DENV and ZIKV.
Epigallocatechin-3-gallate (EGCG) Alters Histone Acetylation and Methylation and Impacts Chromatin Architecture Profile in Human Endothelial Cells
Authors: Ciesielski Oskar , Biesiekierska Marta , Balcerczyk Aneta
Published: May 2020

Epigallocatechin gallate (EGCG), the main green tea polyphenol, exerts a wide variety of biological actions. Epigenetically, the catechin has been classified as a DNMTs inhibitor, however, its impact on histone modifications and chromatin structure is still poorly understood. The purpose of this study was to find the impact of EGCG on the histone posttranslational modifications machinery and chromatin remodeling in human endothelial cells of both microvascular (HMEC-1) and vein (HUVECs) origin. We analyzed the methylation and acetylation status of histones (Western blotting), as well as assessed the activity (fluorometric assay kit) and gene expression (qPCR) of the enzymes playing a prominent role in shaping the human epigenome. The performed analyses showed that EGCG increases histone acetylation (H3K9/14ac, H3ac), and methylation of both active (H3K4me3) and repressive (H3K9me3) chromatin marks. We also found that the catechin acts as an HDAC inhibitor in cellular and cell-free models. Additionally, we observed that EGCG affects chromatin architecture by reducing the expression of heterochromatin binding proteins: HP1α, HP1γ. Our results indicate that EGCG promotes chromatin relaxation in human endothelial cells and presents a broad epigenetic potential affecting expression and activity of epigenome modulators including HDAC5 and 7, p300, CREBP, LSD1 or KMT2A.
Tuning Properties of Partially Reduced Graphene Oxide Fibers upon Calcium Doping
Authors: Tadyszak Krzysztof , Wychowaniec Jacek K. , Załęski Karol , Coy Emerson , Majchrzycki Łukasz , Carmieli Raanan
Published: May 2020

The arrangement of two-dimensional graphene oxide sheets has been shown to influence physico-chemical properties of the final bulk structures. In particular, various graphene oxide microfibers remain of high interest in electronic applications due to their wire-like thin shapes and the ease of hydrothermal fabrication. In this research, we induced the internal ordering of graphene oxide flakes during typical hydrothermal fabrication via doping with Calcium ions (~6 wt.%) from the capillaries. The Ca2+ ions allowed for better graphene oxide flake connections formation during the hydrogelation and further modified the magnetic and electric properties of structures compared to previously studied aerogels. Moreover, we observed the unique pseudo-porous fiber structure and flakes connections perpendicular to the long fiber axis. Pulsed electron paramagnetic resonance (EPR) and conductivity measurements confirmed the denser flake ordering compared to previously studied aerogels. These studies ultimately suggest that doping graphene oxide with Ca2+ (or other) ions during hydrothermal methods could be used to better control the internal architecture and thus tune the properties of the formed structures.
Assessing the Photocatalytic Degradation of Fluoroquinolone Norfloxacin by Mn:ZnS Quantum Dots: Kinetic Study, Degradation Pathway and Influencing Factors
Authors: Patel Jyoti , Singh Ajaya K. , Carabineiro Sónia. A. C.
Published: May 2020

Norfloxacin (NOFX), a broadly used fluoroquinolone antibiotic, has been a subject of great concern in the past few years due to its undesirable effect on human beings and aquatic ecosystems. In this study, novel Mn doped ZnS (Mn:ZnS) quantum dots (QDs) were prepared through a facile chemical precipitation method and used as photocatalysts for NOFX degradation. Prior to photodegradation experiments, morphological and optical parameters of the QDs were examined through transmission electron microscopy, scanning electron microscopy, energy dispersive X-ray analysis, Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, fluorescence spectroscopy, Brunauer–Emmett–Teller analysis, and differential thermal and thermogravimetric analyses. Mn:ZnS QDs exhibited excellent properties of photodegradation, not only under UV irradiation but also in sunlight, which induced NOFX to photodegrade. The utmost photodegradation efficiency was obtained under optimal conditions (25 mL of NOFX, 15 mg/L, pH 10, 60 min UV irradiation, 60 mgs QDs), adopting first order kinetics. In addition, hydroxyl radicals produced by the conduction band electrons were found to be the primary reason dominating the transformation of NOFX in basic conditions, while holes, oxygen atoms, as well as the doped metal (Mn) enhanced the degradation. The QDs showed excellent reusability and stability in four repeated cycles. Finally, four different pathways were predicted, derived from the identified intermediates, with piperazinyl ring transformation being the primary one. It is expected that the synthesized Mn:ZnS QDs could be utilized as efficient photocatalytic materials for energy conversion and ecological remediation.
Dietary Recommendations for Bariatric Patients to Prevent Kidney Stone Formation
Authors: Ormanji Milene S. , Rodrigues Fernanda G. , Heilberg Ita P.
Published: May 2020

Bariatric surgery (BS) is one of the most common and efficient surgical procedures for sustained weight loss but is associated with long-term complications such as nutritional deficiencies, biliary lithiasis, disturbances in bone and mineral metabolism and an increased risk of nephrolithiasis, attributed to urinary metabolic changes resultant from low urinary volume, hypocitraturia and hyperoxaluria. The underlying mechanisms responsible for hyperoxaluria, the most common among all metabolic disturbances, may comprise increased intestinal oxalate absorption consequent to decreased calcium intake or increased dietary oxalate, changes in the gut microbiota, fat malabsorption and altered intestinal oxalate transport. In the current review, the authors present a mechanistic overview of changes found after BS and propose dietary recommendations to prevent the risk of urinary stone formation, focusing on the role of dietary oxalate, calcium, citrate, potassium, protein, fat, sodium, probiotics, vitamins D, C, B6 and the consumption of fluids.
Immunomodulatory Effects of Vitamin D in Thyroid Diseases
Authors: Mele Chiara , Caputo Marina , Bisceglia Alessandro , Samà Maria Teresa , Zavattaro Marco , Aimaretti Gianluca , Pagano Loredana , Prodam Flavia , Marzullo Paolo
Published: May 2020

Vitamin D is a secosteroid with a pleiotropic role in multiple physiological processes. Besides the well-known activity on bone homeostasis, recent studies suggested a peculiar role of vitamin D in different non-skeletal pathways, including a key role in the modulation of immune responses. Recent evidences demonstrated that vitamin D acts on innate and adaptative immunity and seems to exert an immunomodulating action on autoimmune diseases and cancers. Several studies demonstrated a relationship between vitamin D deficiency, autoimmune thyroid disorders, and thyroid cancer. This review aims to summarize the evidences on the immunomodulatory effect of vitamin D on thyroid diseases.
Gut-Muscle Axis Exists and May Affect Skeletal Muscle Adaptation to Training
Authors: Przewłócka Katarzyna , Folwarski Marcin , Kaźmierczak-Siedlecka Karolina , Skonieczna-Żydecka Karolina , Kaczor Jan Jacek
Published: May 2020

Excessive training may limit physiological muscle adaptation through chronic oxidative stress and inflammation. Improper diet and overtraining may also disrupt intestinal homeostasis and in consequence enhance inflammation. Altogether, these factors may lead to an imbalance in the gut ecosystem, causing dysregulation of the immune system. Therefore, it seems to be important to optimize the intestinal microbiota composition, which is able to modulate the immune system and reduce oxidative stress. Moreover, the optimal intestinal microbiota composition may have an impact on muscle protein synthesis and mitochondrial biogenesis and function, as well as muscle glycogen storage. Aproperly balanced microbiome may also reduce inflammatory markers and reactive oxygen species production, which may further attenuate macromolecules damage. Consequently, supplementation with probiotics may have some beneficial effect on aerobic and anaerobic performance. The phenomenon of gut-muscle axis should be continuously explored to function maintenance, not only in athletes.
Dietary Habits and Nutrition in Rheumatoid Arthritis: Can Diet Influence Disease Development and Clinical Manifestations?
Authors: Gioia Chiara , Lucchino Bruno , Tarsitano Maria Grazia , Iannuccelli Cristina , Di Franco Manuela
Published: May 2020

Rheumatoid arthritis (RA) is a systemic, autoimmune disease characterized by joint involvement, with progressive cartilage and bone destruction. Genetic and environmental factors determine RA susceptibility. In recent years, an increasing number of studies suggested that diet has a central role in disease risk and progression. Several nutrients, such as polyunsaturated fatty acids, present anti-inflammatory and antioxidant properties, featuring a protective role for RA development, while others such as red meat and salt have a harmful effect. Gut microbiota alteration and body composition modifications are indirect mechanisms of how diet influences RA onset and progression. Possible protective effects of some dietary patterns and supplements, such as the Mediterranean Diet (MD), vitamin D and probiotics, could be a possible future adjunctive therapy to standard RA treatment. Therefore, a healthy lifestyle and nutrition have to be encouraged in patients with RA.
Higher Cardiorespiratory Fitness Levels May Attenuate the Detrimental Association between Weight Status, Metabolic Phenotype and C-Reactive Protein in Adolescents—A Multi-Cohort Study
Authors: Agostinis-Sobrinho Cesar , Rosário Rafaela , Santos Rute , Norkiene Sigute , Mota Jorge , Rauckienė-Michaelsson Alona , González-Ruíz Katherine , Izquierdo Mikel , Garcia-Hermoso Antonio , Ramírez-Vélez Robinson
Published: May 2020

Results from several studies show that only obese, unfit subjects, but not obese, fit subjects, are at higher mortality risk than are normal-weight fit subjects. The aim of the study was two-fold: (1) to examine the differences in C-reactive protein levels across different metabolic phenotypes (healthy and unhealthy) of weight status and (2) ascertain whether high levels of cardiorespiratory fitness (CRF) attenuate the association of C-reactive protein and metabolic phenotypes of weight status. This was a pooled study, which included data from three cross-sectional projects (1706 youth (921 girls) aged 12–18 years). We used a Shuttle run test to assess CRF. Adolescents were classified into six metabolic phenotypes (healthy and unhealthy) of weight status (non-overweight, overweight and obese), based on age- and sex-specific cutoff points for triglycerides, systolic blood pressure, HDL-cholesterol, glucose and body mass index. High-sensitivity assays were used to obtain the C-reactive protein as inflammatory biomarker. After adjustment for potential confounders (age, sex, pubertal stage and country), the analysis of covariance (ANCOVA) shows that C-reactive protein is directly associated with metabolic phenotypes of weight status. Subjects with obesity, regardless of their metabolic profile, had higher levels of C-reactive protein Z-score. In addition, (after adjustments for potential confounders) a two-way ANCOVA showed that high levels of CRF attenuated the associations of C-reactive protein levels in metabolic healthy non-overweight and in adolescents with obesity. In conclusion, higher CRF levels may attenuate the detrimental association between obesity and C-reactive protein independently of metabolic phenotype. Findings from this study are important for prevention, clinical practice on issues associated with adiposity and metabolic disorders.
From Signaling Pathways to Distinct Immune Responses: Key Factors for Establishing or Combating Neospora caninum Infection in Different Susceptible Hosts
Authors: Fereig Ragab M. , Nishikawa Yoshifumi
Published: May 2020

Neospora caninum is an intracellular protozoan parasite affecting numerous animal species. It induces significant economic losses because of abortion and neonatal abnormalities in cattle. In case of infection, the parasite secretes numerous arsenals to establish a successful infection in the host cell. In the same context but for a different purpose, the host resorts to different strategies to eliminate the invading parasite. During this battle, numerous key factors from both parasite and host sides are produced and interact for the maintaining and vanishing of the infection, respectively. Although several reviews have highlighted the role of different compartments of the immune system against N. caninum infection, each one of them has mostly targeted specific points related to the immune component and animal host. Thus, in the current review, we will focus on effector molecules derived from the host cell or the parasite using a comprehensive survey method from previous reports. According to our knowledge, this is the first review that highlights and discusses immune response at the host cell–parasite molecular interface against N. caninum infection in different susceptible hosts.
Streptococcus suis Uptakes Carbohydrate Source from Host Glycoproteins by N-glycans Degradation System for Optimal Survival and Full Virulence during Infection
Authors: Ma Jiale , Zhang Ze , Pan Zihao , Bai Qiankun , Zhong Xiaojun , Zhu Yinchu , Zhang Yue , Wu Zongfu , Liu Guangjin , Yao Huochun
Published: May 2020

Infection with the epidemic virulent strain of Streptococcus suis serotype 2 (SS2) can cause septicemia in swine and humans, leading to pneumonia, meningitis and even cytokine storm of Streptococcal toxic shock-like syndrome. Despite some progress concerning the contribution of bacterial adhesion, biofilm, toxicity and stress response to the SS2 systemic infection, the precise mechanism underlying bacterial survival and growth within the host bloodstream remains elusive. Here, we reported the SS2 virulent strains with a more than 20 kb endoSS-related insertion region that showed significantly higher proliferative ability in swine serum than low-virulent strains. Further study identified a complete N-glycans degradation system encoded within this insertion region, and found that both GH92 and EndoSS contribute to bacterial virulence, but that only DndoSS was required for optimal growth of SS2 in host serum. The supplement of hydrolyzed high-mannose-containing glycoprotein by GH92 and EndoSS could completely restore the growth deficiency of endoSS deletion mutant in swine serum. EndoSS only hydrolyzed a part of the model glycoprotein RNase B with high-mannose N-linked glycoforms into a low molecular weight form, and the solo activity of GH92 could not show any changes comparing with the blank control in SDS-PAGE gel. However, complete hydrolyzation was observed under the co-incubation of EndoSS and GH92, suggesting GH92 may degrade the high-mannose arms of N-glycans to generate a substrate for EndoSS. In summary, these findings provide compelling evidences that EndoSS-related N-glycans degradation system may enable SS2 to adapt to host serum-specific availability of carbon sources from glycoforms, and be required for optimal colonization and full virulence during systemic infection.
Upregulated and Hyperactivated Thalamic Connexin 43 Plays Important Roles in Pathomechanisms of Cognitive Impairment and Seizure of Autosomal Dominant Sleep-Related Hypermotor Epilepsy with S284L-Mutant α4 Subunit of Nicotinic ACh Receptor
Authors: Fukuyama Kouji , Fukuzawa Masashi , Okada Motohiro
Published: May 2020

To understand the pathomechanism and pathophysiology of autosomal dominant sleep-related hypermotor epilepsy (ADSHE), we studied functional abnormalities of glutamatergic transmission in thalamocortical pathway from reticular thalamic nucleus (RTN), mediodorsal thalamic nucleus (MDTN) to orbitofrontal cortex (OFC) associated with S286L-mutant α4β2-nicotinic acetylcholine receptor (nAChR), and connexin43 (Cx43) hemichannel of transgenic rats bearing rat S286L-mutant Chrna4 gene (S286L-TG), corresponding to the human S284L-mutant CHRNA4 gene using simple Western analysis and multiprobe microdialysis. Cx43 expression in the thalamic plasma membrane fraction of S286L-TG was upregulated compared with that of wild-type. Subchronic administrations of therapeutic-relevant doses of zonisamide (ZNS) and carbamazepine (CBZ) decreased and did not affect Cx43 expression of S286L-TG, respectively. Upregulated Cx43 enhanced glutamatergic transmission during both resting and hyperexcitable stages in S286L-TG. Furthermore, activation of GABAergic transmission RTN–MDTN pathway conversely enhanced, but not inhibited, l-glutamate release in the MDTN via upregulated/activated Cx43. Local administration of therapeutic-relevant concentration of ZNS and CBZ acutely supressed and did not affect glutamatergic transmission in the thalamocortical pathway, respectively. These results suggest that pathomechanisms of ADSHE seizure and its cognitive deficit comorbidity, as well as pathophysiology of CBZ-resistant/ZNS-sensitive ADSHE seizures of patients with S284L-mutation.
Enhancement of the Physical Stability of Amorphous Sildenafil in a Binary Mixture, with either a Plasticizing or Antiplasticizing Compound
Authors: Knapik-Kowalczuk Justyna , Chmiel Krzysztof , Pacułt Justyna , Bialek Klaudia , Tajber Lidia , Paluch Marian
Published: May 2020

The main purpose of this paper was to evaluate the impact of both high- and low-Tg polymer additives on the physical stability of an amorphous drug, sildenafil (SIL). The molecular mobility of neat amorphous SIL was strongly affected by the polymeric excipients used (Kollidon VA64 (KVA) and poly(vinylacetate) (PVAc)). The addition of KVA slowed down the molecular dynamics of amorphous SIL (antiplasticizing effect), however, the addition of PVAc accelerated the molecular motions of the neat drug (plasticizing effect). Therefore, in order to properly assess the effect of the polymer on the physical stability of SIL, the amorphous samples at both: isothermal (at constant temperature—353 K) and isochronal (at constant relaxation time—τα = 1.5 ms) conditions were compared. Our studies showed that KVA suppressed the recrystallization of amorphous SIL more efficiently than PVAc. KVA improved the physical stability of the amorphous drug, regardless of the chosen concentration. On the other hand, in the case of PVAc, a low polymer content (i.e., 25 wt.%) destabilized amorphous SIL, when stored at 353 K. Nevertheless, at high concentrations of this excipient (i.e., 75 wt.%), its effect on the amorphous pharmaceutical seemed to be the opposite. Therefore, above a certain concentration, the PVAc presence no longer accelerates the SIL recrystallization process, but inhibits it.