bluuebunny/crossref_metadata_2025 huggingface dataset:
Individuelle Digitalisierungsbereitschaft in der Praxis
Authors: Muehlburger Manuel
Published: November 2020

Digitale Transformation verändert die Arbeitsrealitäten für Mitarbeiter und Mitarbeiterinnen in Organisationen. Die zunehmende Einbindung von Technologie in den Arbeitsalltag verändert dabei auch die Anforderungen an die Mitarbeiterinnen und Mitarbeiter. Die vorliegende Studie beschäftigt sich mit der Frage, welche Eigenschaften die Belegschaft befähigen die digitale Transformation ihrer Organisation zu unterstützen und mittels welcher Förderansätze diese Eigenschaften entwickelt werden können. Basierend auf einer initialen Literaturanalyse und einer qualitativen Querschnittsstudie bei zehn österreichischen Unternehmen wurden grundlegende Eigenschaften von digitalisierungsbereiten Mitarbeiterinnen und Mitarbeitern erhoben und bestehende Ansätze zur Förderung der individuellen Digitalisierungsbereitschaft analysiert. Die Studie zeigt, dass individuelle Digitalisierungsbereitschaft in der Praxis als dynamisches Konzept mit starken Abhängigkeiten von organisationalen und individuellen Faktoren gesehen wird. Im Zentrum des Konzepts stehen die Einstellung des Individuums gegenüber Neuem, die Einstellung zu Technologie sowie die Fähigkeit für ganzheitliches Verständnis und Handeln im organisationalen Kontext. Förderungsansätze fokussieren darauf, bestehende Ängste im Hinblick auf Technologie abzubauen, den möglichen Nutzen von Technologie für Mitarbeiter aufzuzeigen und Innovation am Arbeitsplatz zu ermöglichen. Zusätzlich setzen Organisationen auf gezielte Initiativen in Form von technischen, wertezentrierten und hybriden Schulungskonzepten.
Morbidity management and surveillance of lymphatic filariasis disease and acute dermatolymphangioadenitis attacks using a mobile phone-based tool by community health volunteers in Ghana
Authors: Debrah Linda Batsa , Mohammed Aliyu , Osei-Mensah Jubin , Mubarik Yusif , Agbenyega Olivia , Ayisi-Boateng Nana Kwame , Pfarr Kenneth , Kuehlwein Janina Melanie , Klarmann-Schulz Ute , Hoerauf Achim , Debrah Alexander Yaw
Published: November 2020

Morbidity burden of lymphatic filariasis (LF) relies on the information from the Mass Drug Administration (MDA) programme where Community Health Volunteers (CHVs) passively report cases identified. Consequently, the exact prevalence of morbidity cases is not always accurate. The use of mobile phone technology to report morbidity cases was piloted in Ghana using a text-based short messaging service (SMS) tool by CHVs. Though successful, illiterate CHVs could not effectively use the SMS tool. The aim of this study was to evaluate the use of a mobile phone-based Interactive Voice Response System (mIVRS) by CHVs in reporting LF morbidity cases and acute dermatolymphangioadenitis (ADLA) attacks in Ghana. The mIVRS was designed as a surveillance tool to capture LF data in Kassena Nankana Districts of Ghana. One hundred CHVs were trained to identify and report lymphedema and hydrocele cases as well as ADLA attacks by calling a hotline linked to the mIVRS. The system asked a series of questions about the disease condition. The ability of the CHV to report accurately was assessed and the data from the mIVRS were compared with the paper records from the CHVs and existing MDA programme records from the same communities and period. Higher numbers of lymphedema and hydrocele cases were recorded by the CHVs using the mIVRS (n = 590 and n = 103) compared to the paper-based reporting (n = 417 and n = 76) and the MDA records (n = 154 and n = 84). Female CHVs, CHVs above 40 years, and CHVs with higher educational levels were better at paper-based reporting (P = 0.007, P = 0.001, P = 0.049 respectively). The system, when fully developed and linked to national databases, may help to overcome underreporting of morbidity cases and ADLA attacks in endemic communities. The system has the potential to be further expanded to other diseases.
The furnace and the goat—A spatio-temporal model of the fuelwood requirement for iron metallurgy on Elba Island, 4th century BCE to 2nd century ce
Authors: Becker Fabian , Djurdjevac Conrad Nataša , Eser Raphael A. , Helfmann Luzie , Schütt Brigitta , Schütte Christof , Zonker Johannes
Published: November 2020

Scholars frequently cite fuel scarcity after deforestation as a reason for the abandonment of most of the Roman iron smelting sites on Elba Island (Tuscan Archipelago, Italy) in the 1st centurybce. Whereas the archaeological record clearly indicates the decrease in smelting activities, evidence confirming the ‘deforestation narrative’ is ambiguous. Therefore, we employed a stochastic, spatio-temporal model of the wood required and consumed for iron smelting on Elba Island in order to assess the availability of fuelwood on the island. We used Monte Carlo simulations to cope with the limited knowledge available on the past conditions on Elba Island and the related uncertainties in the input parameters. The model includes both, wood required for the furnaces and to supply the workforce employed in smelting. Although subject to high uncertainties, the outcomes of our model clearly indicate that it is unlikely that all woodlands on the island were cleared in the 1st centurybce. A lack of fuel seems only likely if a relatively ineffective production process is assumed. Therefore, we propose taking a closer look at other reasons for the abandonment of smelting sites, e.g. the occupation of new Roman provinces with important iron ore deposits; or a resource-saving strategy inItalia. Additionally, we propose to read the development of the ‘deforestation narrative’ originating from the 18th/19th century in its historical context.
The genetic paradigms of dietary restriction fail to extend life span in cep-1(gk138) mutant of C. elegans p53 due to possible background mutations
Authors: Goyala Anita , Baruah Aiswarya , Mukhopadhyay Arnab
Published: November 2020

Dietary restriction (DR) increases life span and improves health in most model systems tested, including non-human primates. In C. elegans, as in other models, DR leads to reprogramming of metabolism, improvements in mitochondrial health, large changes in expression of cytoprotective genes and better proteostasis. Understandably, multiple global transcriptional regulators like transcription factors FOXO/DAF-16, FOXA/PHA-4, HSF1/HSF-1 and NRF2/SKN-1 are important for DR longevity. Considering the wide-ranging effects of p53 on organismal biology, we asked whether the C. elegans ortholog, CEP-1 is required for DR-mediated longevity assurance. We employed the widely-used TJ1 strain of cep-1(gk138). We show that cep-1(gk138) suppresses the life span extension of two genetic paradigms of DR, but two non-genetic modes of DR remain unaffected in this strain. We find that two aspects of DR, increased autophagy and up-regulation of the expression of cytoprotective xenobiotic detoxification program (cXDP) genes, are dampened in cep-1(gk138). Importantly, we find that background mutation(s) in the strain may be the actual cause for the phenotypic differences that we observed and cep-1 may not be directly involved in genetic DR-mediated longevity assurance in worms. Identifying these mutation(s) may reveal a novel regulator of longevity required specifically by genetic modes of DR.
Chikungunya virus superinfection exclusion is mediated by a block in viral replication and does not rely on non-structural protein 2
Authors: Boussier Jeremy , Levi Laura , Weger-Lucarelli James , Poirier Enzo Z. , Vignuzzi Marco , Albert Matthew L.
Published: November 2020

Superinfection exclusion (SIE) is a process by which a virally infected cell is protected from subsequent infection by the same or a closely related virus. By preventing cell coinfection, SIE favors preservation of genome integrity of a viral strain and limits its recombination potential with other viral genomes, thereby impacting viral evolution. Although described in virtually all viral families, the precise step(s) impacted by SIE during the viral life cycle have not been systematically explored. Here, we describe for the first time SIE triggered by chikungunya virus (CHIKV), an alphavirus of public health importance. Using single-cell technologies, we demonstrate that CHIKV excludes subsequent infection with: CHIKV; Sindbis virus, a related alphavirus; and influenza A, an unrelated RNA virus. We further demonstrate that SIE does not depend on the action of type I interferon, nor does it rely on host cell transcription. Moreover, exclusion is not mediated by the action of a single CHIKV protein; in particular, we observed no role for non-structural protein 2 (nsP2), making CHIKV unique among characterized alphaviruses. By stepping through the viral life cycle, we show that CHIKV exclusion occurs at the level of replication, but does not directly influence virus binding, nor viral structural protein translation. In sum, we characterized co-infection during CHIKV replication, which likely influences the rate of viral diversification and evolution.
c-Src kinase inhibits osteogenic differentiation via enhancing STAT1 stability
Authors: Alvandi Zahra , Opas Michal
Published: November 2020

The proto-oncogene Src is ubiquitously expressed and is involved in cellular differentiation. However, the role of Src in embryonic stem (ES) cell osteogenic differentiation is largely unknown. Using the small molecule inhibitor PP2, c-Src specific siRNAs, and tet-inducible lentiviral vectors overexpressing active c-Src, we delineated an inhibitory role of c-Src in osteogenic differentiation of mouse embryonic stem cells (mESCs) and mouse MC3T3-E1s preosteoblasts. Active c-Src was shown to restrict the nuclear residency of Runt-related transcription factor 2 (Runx2) and its transcriptional activity with no detectable effect on Runx2 expression level. Furthermore, we showed Signal Transducer and Activator of Transcription 1 (STAT1) was indispensable to the inhibitory role of c-Src on Runx2 nuclear localization. Specifically, higher levels of active c-Src increased STAT1 half-life by inhibiting its proteasomal degradation, thereby increasing the cytoplasmic abundance of STAT1. More abundant cytoplasmic STAT1 bound and anchored Runx2, which restricted its nucleocytoplasmic shuttling and ultimately reduced Runx2 transcriptional activity. Collectively, this study has defined a new mechanism by which c-Src inhibits the transcriptional regulation of osteogenesis from mESCsin vitro.
Detainee and layperson’s expectations and preferences regarding police interview rooms
Authors: Hoogesteyn Katherine , Meijer Ewout H. , Vrij Aldert
Published: November 2020

Emerging research on how suspects perceive the physical environment during investigative interviews yields contrasting findings. While previous studies have suggested that a room made to be physically comfortable may be optimal for interviewing suspects, another study found it can instead lead to higher suspicion of the investigator’s intentions. The current study examined current detainees’ and general population participants’ beliefs about a room that resembled a “typical” interview room, and one decorated to be warm, inviting, and comfortable. Participants also provided descriptive information about their perceptions of police interview environments (e.g., preferences, expectations). We hypothesized that the decorated room would elicit higher ratings of suspicion and wariness compared to the “typical” room. Our findings showed that, overall, participants expected to be interviewed in the “typical” room but preferred the decorated one. Contrary to our expectations, they rated the “typical” room higher on feelings of suspicion than the decorated room. The decorated room also corresponded with what participants reported to be an environment that promotes disclosure. These results bode well for conducting investigative interviews in comfortable environments.
Adaptation of technological packaging for conservation of soybean seeds in storage units as an alternative to modified atmospheres
Authors: Coradi Paulo Carteri , Padia Claudir Lari , Jaques Lanes Beatriz Acosta , Souza Guilherme Abreu Coelho de , Lima Roney Eloy , Müller Amanda , Teodoro Paulo Eduardo , Steinhaus Jonatas Ibagé , Carneiro Letícia de Oliveira
Published: November 2020

This study aimed to evaluate the quality of seeds of RR and RR2 PRO soybean cultivars stored in ambient air with raffia packaging (ANER), ambient air with laminated packaging (ANEL), modified atmosphere with polyethylene packaging (AMEP), refrigerated atmosphere (1 to 3°C) with raffia packaging (ARER), refrigerated atmosphere (1 to 3°C) with laminated packaging (AREL), and modified (-14 PSI) and refrigerated (1 to 3°C) atmosphere with polyethylene packaging (AMREP), over 6 months of storage. Results showed that the seeds of cultivar RR2 were preserved with better physiological quality. Raffia and polyethylene packaging under natural storage conditions, in a refrigerated and modified atmosphere, did not preserve the seed quality over the storage period. The conditions of storage in ambient air with laminated packaging (ANEL) and in a refrigerated atmosphere with laminated packaging (AREL) reduced the environmental effects of temperature and relative humidity, leading to better results of physiological quality of the seeds. Storage time negatively influenced the physiological quality of seeds, except for AREL and ANEL, which maintained the quality close to that of the initial conditions, over the 6 months of storage. The best alternatives for soybean seeds storage over 6 months are the laminated packaging in a natural environment, matching the refrigerated conditions. The technological laminated packaging can be used as a new alternative for conserving soybean seeds in processing and storage units.
First national tuberculosis patient cost survey in Lao People’s Democratic Republic: Assessment of the financial burden faced by TB-affected households and the comparisons by drug-resistance and HIV status
Authors: Chittamany Phonenaly , Yamanaka Takuya , Suthepmany Sakhone , Sorsavanh Thepphouthone , Siphanthong Phitsada , Sebert Jacques , Viney Kerri , Vixaysouk Thipphasone , Nagai Moeko , Seevisay Vilath , Izumi Kiyohiko , Morishita Fukushi , Nishikiori Nobuyuki
Published: November 2020

Background

Tuberculosis (TB) patients incur large costs for care seeking, diagnosis, and treatment. To understand the magnitude of this financial burden and its main cost drivers, the Lao People’s Democratic Republic (PDR) National TB Programme carried out the first national TB patient cost survey in 2018–2019.

Method

A facility-based cross-sectional survey was conducted based on a nationally representative sample of TB patients from public health facilities across 12 provinces. A total of 848 TB patients including 30 drug resistant (DR)-TB and 123 TB-HIV coinfected patients were interviewed using a standardised questionnaire developed by the World Health Organization. Information on direct medical, direct non-medical and indirect costs, as well as coping mechanisms was collected. We estimated the percentage of TB-affected households facing catastrophic costs, which was defined as total TB-related costs accounting for more than 20% of annual household income.

Result

The median total cost of TB care was US$ 755 (Interquartile range 351–1,454). The costs were driven by direct non-medical costs (46.6%) and income loss (37.6%). Nutritional supplements accounted for 74.7% of direct non-medical costs. Half of the patients used savings, borrowed money or sold household assets to cope with TB. The proportion of unemployment more than doubled from 16.8% to 35.4% during the TB episode, especially among those working in the informal sector. Of all participants, 62.6% of TB-affected households faced catastrophic costs. This proportion was higher among households with DR-TB (86.7%) and TB-HIV coinfected patients (81.1%).

Conclusion

In Lao PDR, TB patients and their households faced a substantial financial burden due to TB, despite the availability of free TB services in public health facilities. As direct non-medical and indirect costs were major cost drivers, providing free TB services is not enough to ease this financial burden. Expansion of existing social protection schemes to accommodate the needs of TB patients is necessary.
A highly nonlinear substitution-box (S-box) design using action of modular group on a projective line over a finite field
Authors: Siddiqui Nasir , Yousaf Fahim , Murtaza Fiza , Ehatisham-ul-Haq Muhammad , Ashraf M. Usman , Alghamdi Ahmed M. , Alfakeeh Ahmed S.
Published: November 2020

Cryptography is commonly used to secure communication and data transmission over insecure networks through the use of cryptosystems. A cryptosystem is a set of cryptographic algorithms offering security facilities for maintaining more cover-ups. A substitution-box (S-box) is the lone component in a cryptosystem that gives rise to a nonlinear mapping between inputs and outputs, thus providing confusion in data. An S-box that possesses high nonlinearity and low linear and differential probability is considered cryptographically secure. In this study, a new technique is presented to construct cryptographically strong 8×8 S-boxes by applying an adjacency matrix on the Galois fieldGF(28). The adjacency matrix is obtained corresponding to the coset diagram for the action of modular groupPSL(2,Z)on a projective linePL(F7) over a finite fieldF7. The strength of the proposed S-boxes is examined by common S-box tests, which validate their cryptographic strength. Moreover, we use the majority logic criterion to establish an image encryption application for the proposed S-boxes. The encryption results reveal the robustness and effectiveness of the proposed S-box design in image encryption applications.
Mixed infections by different Trypanosoma cruzi discrete typing units among Chagas disease patients in an endemic community in Panama
Authors: Ledezma Alexa Prescilla , Blandon Roberto , Schijman Alejandro G. , Benatar Alejandro , Saldaña Azael , Osuna Antonio
Published: November 2020

Background

Trypanosoma cruzi, the hemoparasite that causes Chagas disease, is divided into six Discrete Typing Units or DTUs: TcI-TcVI plus Tcbat. This genetic diversity is based on ecobiological and clinical characteristics associated with particular populations of the parasite. The main objective of this study was the identification of DTUs in patients with chronic chagasic infections from a mountainous rural community in the eastern region of Panama.

Methods

A total of 106 patients were tested for Chagas disease with three serological tests (ELISA, rapid test, and Western blot). Molecular diagnosis and DTU typing were carried out by conventional PCRs and qPCR targeting different genomic markers, respectively. As a control sample for the typing, 28 patients suspected to be chagasic from the metropolitan area of Panama City were included.

Results

Results showed a positivity in the evaluated patients of 42.3% (33/78); high compared to other endemic regions in the country. In the control group, 20/28 (71.43%) patients presented positive serology. The typing of samples from rural patients showed that 78.78% (26/33) corresponded to TcI, while 9.09% (3/33) were mixed infections (TcI plus TcII/V/VI). Seventy-five percent (15/20) of the patients in the control group presented TcI, and in five samples it was not possible to typify the T. cruzi genotype involved.

Conclusions

These results confirm that TcI is the main DTU of T. cruzi present in chronic chagasic patients from Panama. However, the circulation of other genotypes (TcII/V/VI) in this country is described for the first time. The eco-epidemiological characteristics that condition the circulation of TcII/V/VI, as well as the immune and clinical impact of mixed infections in this remote mountainous region should be investigated, which will help local action programs in the surveillance, prevention, and management of Chagas disease.
High genetic diversity of ancient horses from the Ukok Plateau
Authors: Vorobieva Nadezhda V. , Makunin Alexey I. , Druzhkova Anna S. , Kusliy Mariya A. , Trifonov Vladimir A. , Popova Kseniya O. , Polosmak Natalia V. , Molodin Vyacheslav I. , Vasiliev Sergei K. , Shunkov Michael V. , Graphodatsky Alexander S.
Published: November 2020

A growing number of researchers studying horse domestication come to a conclusion that this process happened in multiple locations and involved multiple wild maternal lines. The most promising approach to address this problem involves mitochondrial haplotype comparison of wild and domestic horses from various locations coupled with studies of possible migration routes of the ancient shepherds. Here, we sequenced complete mitochondrial genomes of six horses from burials of the Ukok plateau (Russia, Altai Mountains) dated from 2.7 to 1.4 thousand years before present and a single late Pleistocene wild horse from the neighboring region (Denisova cave). Sequencing data indicates that the wild horse belongs to an extinct pre-domestication lineage. Integration of the domestic horse data with known Eurasian haplotypes of a similar age revealed two distinct groups: the first one widely distributed in Europe and presumably imported to Altai, and the second one specific for Altai Mountains and surrounding area.
Health education improves referral compliance of persons with probable Diabetic Retinopathy: A randomized controlled trial
Authors: Khair Zara , Rahman Md Moshiur , Kazawa Kana , Jahan Yasmin , Faruque Abu S. G. , Chisti Mohammod Jobayer , Moriyama Michiko
Published: November 2020

Objective

Lack of awareness about Diabetic Retinopathy (DR) is the most commonly cited reason why many persons with type 2 diabetes are non-compliant with referral instruction to undergo retinal screening. The purpose of this study was to evaluate the efficacy of a culturally, geographically and socially appropriate, locally adapted five-month-long health education on referral compliance of participants.

Method

A prospective randomized, open-label parallel group study was conducted on persons with type 2 diabetes who underwent basic eye screening at a diabetes hospital between September 2017 and August 2018. Participants who were noncompliant with referral instruction to visit a hospital for advanced DR management were randomly divided into health education intervention group (n = 143) and control group (n = 156). Both groups received information regarding DR and referral instruction at the diabetes hospital. The intervention group was provided personalized education followed by telephonic reminders. The primary endpoint was ‘increase in referral compliance’ and the secondary endpoint was ‘increase in knowledge of DR’. Multivariate logistic regression model was used to identify significant predictors of compliance to referral.

Results

A total of nine participants dropped and 290 completed the post intervention survey. The compliance rate in intervention group was found to be significantly higher than the control group (64.3% vs 28.2%; OR 4.73; 95% CI 2.87–7.79; p<0.001). Participants in the intervention group acquired better knowledge on DR (p<0.05). Apart from intervention, referral compliance rate was also found to be significantly associated with participants’ self-perception of vision problem (OR 2.02; 95% CI 1.02–4.01; p = 0.045) and participants’ income (OR 1.24; 95% CI 1.06–1.44; p = 0.008).

Discussion

Our results suggest that intensive health education on DR should be integrated with diabetes education as it may result in significantly improved referral compliance. Outcomes may be sustainable if interventions are institutionalized at referral point.

Trial registration

Clinical Trials.gov (Registration # NCT03658980);https://clinicaltrials.gov/ct2/show/NCT03658980.
A versatile framework for resource-limited sentiment articulation, annotation, and analysis of short texts
Authors: Batanović Vuk , Cvetanović Miloš , Nikolić Boško
Published: November 2020

Choosing a comprehensive and cost-effective way of articulating and annotating the sentiment of a text is not a trivial task, particularly when dealing with short texts, in which sentiment can be expressed through a wide variety of linguistic and rhetorical phenomena. This problem is especially conspicuous in resource-limited settings and languages, where design options are restricted either in terms of manpower and financial means required to produce appropriate sentiment analysis resources, or in terms of available language tools, or both. In this paper, we present a versatile approach to addressing this issue, based on multiple interpretations of sentiment labels that encode information regarding the polarity, subjectivity, and ambiguity of a text, as well as the presence of sarcasm or a mixture of sentiments. We demonstrate its use on Serbian, a resource-limited language, via the creation of a main sentiment analysis dataset focused on movie comments, and two smaller datasets belonging to the movie and book domains. In addition to measuring the quality of the annotation process, we propose a novel metric to validate its cost-effectiveness. Finally, the practicality of our approach is further validated by training, evaluating, and determining the optimal configurations of several different kinds of machine-learning models on a range of sentiment classification tasks using the produced dataset.
Mental health and quality of life of patients with osteoarthritis pain: The sixth Korea National Health and Nutrition Examination Survey (2013–2015)
Authors: Lee Yura , Lee Sook-Hyun , Lim Sung Min , Baek Seung Ho , Ha In-Hyuk
Published: November 2020

Objectives

This study aims to investigate the association between mental health and quality of life of osteoarthritis (OA) patients according to the site of pain.

Design

Retrospective cross-sectional study

Participants

Data of 22,948 participants of the sixth Korea National Health and Nutrition Examination Survey conducted from 2013–2015 were used.

Outcome measures

Participants were asked if they had OA pain in the hip joint, knee joint, and lower back (yes/no) and whether they experienced anxiety or depression. The EQ-5D questionnaire was used to determine the quality of life of patients with hip, knee, and lower back OA. Multiple logistic regression analysis was performed after adjusting.

Results

A total of 5,401 patients reported pain in the hip joint, knee joint, or lower back. The analysis showed significant relations between pain sites, mental health, and quality of life. First, more female patients with OA experienced stress and depression than males. Second, for males with OA, stress was reported in the order of: lower back > hip > knee, while pain and depression was reported in the order of: lower back > knee > hip (p < 0.05). For females with OA, stress was reported in the order of: knee > lower back > hip, while depression was reported in the order of: knee > lower back > hip. Third, considering quality of life, for males, hip joint pain had the greatest impact on quality of life and for females, knee joint pain had the largest impact (p < 0.001).

Conclusions

For patients with OA, the effect on the mental health and quality of life differed according to sex and sites of pain. Therefore, this study confirms that pain sites, sex, mental health, and quality of life are independent risk factors when determining OA pain.
Improved soluble expression and use of recombinant human renalase
Authors: Morrison Clifford S. , Paskaleva Elena E. , Rios Marvin A. , Beusse Thomas R. , Blair Elaina M. , Lin Lucy Q. , Hu James R. , Gorby Aidan H. , Dodds David R. , Armiger William B. , Dordick Jonathan S. , Koffas Mattheos A. G.
Published: November 2020

Electrochemical bioreactor systems have enjoyed significant attention in the past few decades, particularly because of their applications to biobatteries, artificial photosynthetic systems, and microbial electrosynthesis. A key opportunity with electrochemical bioreactors is the ability to employ cofactor regeneration strategies critical in oxidative and reductive enzymatic and cell-based biotransformations. Electrochemical cofactor regeneration presents several advantages over other current cofactor regeneration systems, such as chemoenzymatic multi-enzyme reactions, because there is no need for a sacrificial substrate and a recycling enzyme. Additionally, process monitoring is simpler and downstream processing is less costly. However, the direct electrochemical reduction of NAD(P)+on a cathode may produce adventitious side products, including isomers of NAD(P)H that can act as potent competitive inhibitors to NAD(P)H-requiring enzymes such as dehydrogenases. To overcome this limitation, we examined how nature addresses the adventitious formation of isomers of NAD(P)H. Specifically, renalases are enzymes that catalyze the oxidation of 1,2- and 1,6-NAD(P)H to NAD(P)+, yielding an effective recycling of unproductive NAD(P)H isomers. We designed several mutants of recombinant human renalase isoform 1 (rhRen1), expressed them inE.coliBL21(DE3) to enhance protein solubility, and evaluated the activity profiles of the renalase variants against NAD(P)H isomers. The potential for rhRen1 to be employed in engineering applications was then assessed in view of the enzyme’s stability upon immobilization. Finally, comparative modeling was performed to assess the underlying reasons for the enhanced solubility and activity of the mutant enzymes.
Estimated 24-hour urinary sodium excretion as a risk factor for oxidative stress in Zambian adults: A cross-sectional study
Authors: Kayamba Violet , Kelly Paul
Published: November 2020

Introduction

Persistent oxidative stress predisposes to various non-communicable diseases (NCDs), whose occurrence is increasing in sub-Saharan Africa. The aim of this study was to evaluate the link between markers of oxidative stress and some risk factors for NCDs in a Zambian cohort.

Methods

We assessed oxidative stress by measuring 8-isoprostane (lipid oxidative stress) and 8-hydroxydeoxyguanosine (DNA oxidative stress). In addition, we measured mycotoxins (aflatoxin M1 and ochratoxin A), salt intake estimated from 24-hour sodium excretion calculated using the Tanaka and Kawaski formulae, and 1-hydroxypyrene (a metabolite of polycyclic aromatic hydrocarbons). Data on lifestyle risk factors were collected using questionnaires.

Results

Included were 244 participants; 128 (52%) were female and the median age was 48 years (IQR 39–58). The median level of 8-isoprostane was 0.13 ng/mg creatinine (IQR 0.08–0.23) while that of 8-hydroxydeoxyguanosine (8-OHdG) was 4 ng/mg creatinine (IQR 2–10). The median 24-hour sodium excretion was 21 g (IQR 16–25 g), with none being less than the 5 g recommended by WHO. Unadjusted urinary levels of 8-isoprostane were moderately correlated with 1-hydroxypyrene (Spearman r = 0.30, p<0.001) and estimated 24-hour urine sodium (Spearman r = 0.38, p<0.001). Urinary levels of 8-OHdG were not correlated with 1-hydroxypyrene, estimated 24-hour urine sodium, aflatoxin M1 or ochratoxin A (all p-values >0.05). Using logistic regression, adjusted and unadjusted 8-isoprostanes levels were associated with 1-hydroxypyrene (p = 0.02 and p = 0.001 respectively) and estimated 24-hour urine sodium method (p = 0.003 and p<0.001 respectively). However, only unadjusted 8-OHdG was associated with 1-hydroxypyrene (p = 0.03) and age (p = 0.007).

Conclusions

Estimated 24-hour urinary sodium is high among Zambians and it is associated with lipid but not DNA oxidative stress. High exposure to polycyclic aromatic hydrocarbons is also associated with oxidative stress.
Internal rolling method for particle shape evaluation and reconstruction
Authors: Mo Pin-Qiang
Published: November 2020

A concise and robust method for 2D particle shape evaluation and reconstruction is proposed using the concept of the internal rolling of covering discs within the outline of a particle. By downscaling the covering disc size for capturing multiscale features, the calculation of the Euclidean distance can effectively yield three indices for sphericity, roundness and roughness. The geometric-based evaluations of particle morphology are dimensionless and precisely distinguishable between shapes after calibration and validation against constructed particles and natural sands. A sphericity-roundness diagram is provided to visualize the particle shape characterization, and a probabilistic density surface in the sphericity-roundness diagram is then proposed to statistically represent the distributions of the particle shapes. The concept of internal rolling is also utilized for particle shape reconstruction using a limited number of balls to replicate the indices of sphericity, roundness and roundness characteristic curve. The probabilistic density surface is duplicated for statistical particle shape reconstruction, which provides an effective approach for numerical investigations of the relationships between particle shapes and mechanical behavior. The effect of image quality on 2D shape evaluation is also examined by using a minimum area per particle, and the proposed method is intuitively extendable to 3D measurements using rolling covering spheres.
Clinical significance of IgG antimitochondrial M2 antibody levels in primary biliary cholangitis: A single center study from China
Authors: Feng Lina , Dong Kaihui , Zhang Xiaoxue , Ma Bo , Chen Lin , Yang Qianqian , Chen Qingling , Wen Xiaoyu , Jin Qinglong
Published: November 2020

Background and objective

The relationship between antimitochondrial antibody (AMA) levels and the severity or prognosis of primary biliary cholangitis (PBC) is unclear. This study explored the clinical significance of serum IgG antimitochondrial M2 antibody (IgG-M2) levels.

Methods

From 2008 to 2017, a retrospective analysis was conducted with PBC patients who had available quantitative values of serum IgG-M2 levels obtained with ELISA based on triple expression hybrid clones. The patients were divided into two groups based on high and low concentrations of IgG-M2. Baseline parameters, the incidence of adverse events, and prognosis were compared.

Results

Among the 530 PBC patients, the levels of albumin, cholinesterase, hemoglobin, fibrinogen and triglycerides and the red blood cell count were significantly lower in the high-concentration group than in the low-concentration group (n = 263, 49.6%). The red cell distribution width (RDW) and levels of serum immunoglobulin (Ig) G, IgM and IgA were significantly higher in the high-concentration group than in the low-concentration group. Spearman’s correlation analysis suggested that the correlation between the above baseline indicators and IgG-M2 levels was statistically significant but weak (r < 0.2, P < 0.05). In total, 203 patients were followed up, of whom 87 (42.9%) were in the high-concentration group. During the median follow-up period of 52 months (range: 28–75), 121 (59.6%) experienced hepatic decompensation, and 37 (18.2%) died or underwent liver transplantation. There was no significant difference in the incidence of complications or survival (log-rank test: P = 0.079) between the two groups. One year after ursodeoxycholic acid (UDCA) treatment, the two groups had similar responses. In addition, the levels of IgG-M2 did not fluctuate significantly during treatment.

Conclusion

IgG-M2 levels were not related to the disease severity, prognosis or efficacy of UDCA. The levels of IgG-M2 did not change significantly during treatment.
Development and validation of a time and motion guide to assess the costs of prevention and control interventions for nosocomial infections: A Delphi method among experts
Authors: Tchouaket Nguemeleu Eric , Boivin Sandra , Robins Stephanie , Sia Drissa , Kilpatrick Kelley , Brousseau Sylvain , Dubreuil Bruno , Larouche Catherine , Parisien Natasha
Published: November 2020

Background

Nosocomial infections place a heavy burden on patients and healthcare providers and impact health care institutions financially. Reducing nosocomial infections requires an integrated program of prevention and control using key clinical best care practices. No instrument currently exists that measures these practices in terms of personnel time and material costs.

Objective

To develop and validate an instrument that would measure nosocomial infection control and prevention best care practice costs, including estimates of human and material resources.

Methods

An evaluation of the literature identified four practices essential for the control of pathogens: hand hygiene, hygiene and sanitation, screening and additional precaution. To reflect time, materials and products used in these practices, our team developed a time and motion guide. Iterations of the guide were assessed in a Delphi technique; content validity was established using the content validity index and reliability was assessed using Kruskall Wallis one-way ANOVA of rank test.

Results

Two rounds of Delphi review were required; 88% of invited experts completed the assessment. The final version of the guide contains eight dimensions: Identification [83 items]; Personnel [5 items]; Additional Precautions [1 item]; Hand Hygiene [2 items]; Personal Protective Equipment [14 items]; Screening [4 items]; Cleaning and Disinfection of Patient Care Equipment [33 items]; and Hygiene and Sanitation [24 items]. The content validity index obtained for all dimensions was acceptable (> 80%). Experts statistically agreed on six of the eight dimensions.

Discussion/Conclusion

This study developed and validated a new instrument based on expert opinion, the time and motion guide, for the systematic assessment of costs relating to the human and material resources used in nosocomial infection prevention and control. This guide will prove useful to measure the intensity of the application of prevention and control measures taken before, during and after outbreak periods or during pandemics such as COVID-19.
Short-term and long-term comparisons of laparoscopy-assisted proximal gastrectomy with esophagogastrostomy by the double-flap technique and laparoscopy-assisted total gastrectomy for proximal gastric cancer
Authors: Tsumura Tomoko , Kuroda Shinji , Nishizaki Masahiko , Kikuchi Satoru , Kakiuchi Yoshihiko , Takata Nobuo , Ito Atene , Watanabe Megumi , Kuwada Kazuya , Kagawa Shunsuke , Fujiwara Toshiyoshi
Published: November 2020

Background

Although proximal gastrectomy (PG) is a recognized surgical procedure for early proximal gastric cancer, total gastrectomy (TG) is sometimes selected due to concern about severe gastroesophageal reflux. Esophagogastrostomy by the double-flap technique (DFT) is an anti-reflux reconstruction after PG, and its short-term effectiveness has been reported. However, little is known about the long-term effects on nutritional status and quality of life (QOL).

Methods

Gastric cancer patients who underwent laparoscopy-assisted PG (LAPG) with DFT or laparoscopy-assisted TG (LATG) between April 2011 and March 2014 were retrospectively analyzed. Body weight (BW), body mass index (BMI), and prognostic nutritional index (PNI) were reviewed to assess nutritional status, and the Postgastrectomy Syndrome Assessment Scale (PGSAS)-45 was used to assess QOL.

Results

A total of 36 patients (LATG: 17, LAPG: 19) were enrolled. Four of 17 LATG patients (24%) were diagnosed with Stage ≥II after surgery, and half received S-1 adjuvant chemotherapy. BW and PNI were better maintained in LAPG than in LATG patients until 1-year follow-up. Seven of 16 LATG patients (44%) were categorized as “underweight (BMI<18.5 kg/m2)” at 1-year follow-up, compared to three of 18 LAPG patients (17%; p = 0.0836). The PGSAS-45 showed no significant difference in all QOL categories except for decreased BW (p = 0.0132). Multivariate analysis showed that LATG was the only potential risk factor for severe BW loss (odds ratio: 3.03, p = 0.0722).

Conclusions

LAPG with DFT was superior to LATG in postoperative nutritional maintenance, and can be the first option for early proximal gastric cancer.
Cohort profile: The Chikwawa lung health cohort; a population-based observational non-communicable respiratory disease study of adults in Malawi
Authors: Njoroge Martin W. , Rylance Sarah , Nightingale Rebecca , Gordon Stephen , Mortimer Kevin , Burney Peter , Rylance Jamie , Obasi Angela , Niessen Louis , Devereux Graham ,   on behalf of The IMPALA Consortium
Published: November 2020

Purpose

The aim of this article is to provide a detailed description of the Chikwawa lung health cohort which was established in rural Malawi to prospectively determine the prevalence and causes of lung disease amongst the general population of adults living in a low-income rural setting in Sub-Saharan Africa.

Participants

A total of 1481 participants were randomly identified and recruited in 2014 for the baseline study. We collected data on demographic, socio-economic status, respiratory symptoms and potentially relevant exposures such as smoking, household fuels, environmental exposures, occupational history/exposures, dietary intake, healthcare utilization, cost (medication, outpatient visits and inpatient admissions) and productivity losses. Spirometry was performed to assess lung function. At baseline, 56.9% of the participants were female, mean age was 43.8 (SD:17.8) and mean body mass index (BMI) was 21.6 Kg/m2 (SD: 3.46)

Findings to date

The cohort has reported the prevalence of chronic respiratory symptoms (13.6%, 95% confidence interval [CI], 11.9–15.4), spirometric obstruction (8.7%, 95% CI, 7.0–10.7), and spirometric restriction (34.8%, 95% CI, 31.7–38.0). Additionally, an annual decline in forced expiratory volume in one second [FEV1] of 30.9mL/year (95% CI: 21.6 to 40.1) and forced vital capacity [FVC] by 38.3 mL/year (95% CI: 28.5 to 48.1) has been reported.

Future plans

The ongoing phases of follow-up will determine the annual rate of decline in lung function as measured through spirometry and the development of airflow obstruction and restriction, and relate these to morbidity, mortality and economic cost of airflow obstruction and restriction. Population-based mathematical models will be developed driven by the empirical data from the cohort and national population data for Malawi to assess the effects of interventions and programmes to address the lung burden in Malawi. The present follow-up study started in 2019.
No association between the vitamin D-binding protein (DBP) gene polymorphisms (rs7041 and rs4588) and multiple sclerosis and type 1 diabetes mellitus: A meta-analysis
Authors: Zhang Xin , Gao Bai , Xu Bing
Published: November 2020

Background

The association between polymorphisms in vitamin D-binding protein (DBP) gene and the risk of multiple sclerosis (MS) and type 1 diabetes mellitus (T1DM) has been investigated in many studies, but the studies showed controversial results. The rationale for this meta-analysis was to determine whether DBP polymorphisms increases the risk of MS and T1DM by pooling data.

Methods

Potentially relevant studies were searched using GWAS Catalog, PubMed, Embase, CNKI and WANFANG databases up to November 2019. The pooled odds ratios (ORs) and corresponding 95% confidence intervals (CIs) were performed to estimate the associations in a fixed-effects or random-effects model.

Results

A total of 13 studies were enrolled in this meta-analysis, including eight studies for MS and five for T1DM. The overall results showed that there was no significant association of DBP rs7041 and rs4588 polymorphisms with the risk of MS and T1DM under any genetic model. Similarly, subgroup analysis by ethnicity revealed that no significant association of rs7041 and rs4588 polymorphisms with the risk of MS and T1DM was observed in white or non-white racial groups.

Conclusions

This meta-analysis provides evidence that DBP rs7041 and rs4588 polymorphisms may not be associated with an increased risk in MS and T1DM. However, these findings need further validation by larger-scale epidemiological studies and genome-wide association studies (GWASs) in different populations.
Effects of COVID-19 lockdown on heart rate variability
Authors: Bourdillon Nicolas , Yazdani Sasan , Schmitt Laurent , Millet Grégoire P.
Published: November 2020

Introduction

Strict lockdown rules were imposed to the French population from 17 March to 11 May 2020, which may result in limited possibilities of physical activity, modified psychological and health states. This report is focused on HRV parameters kinetics before, during and after this lockdown period.

Methods

95 participants were included in this study (27 women, 68 men, 37 ± 11 years, 176 ± 8 cm, 71 ± 12 kg), who underwent regular orthostatic tests (a 5-minute supine followed by a 5-minute standing recording of heart rate (HR)) on a regular basis before (BSL), during (CFN) and after (RCV) the lockdown. HR, power in low- and high-frequency bands (LF, HF, respectively) and root mean square of the successive differences (RMSSD) were computed for each orthostatic test, and for each position. Subjective well-being was assessed on a 0–10 visual analogic scale (VAS). The participants were split in two groups, those who reported an improved well-being (WB+, increase >2 in VAS score) and those who did not (WB-) during CFN.

Results

Out of the 95 participants, 19 were classified WB+ and 76 WB-. There was an increase in HR and a decrease in RMSSD when measured supine in CFN and RCV, compared to BSL in WB-, whilst opposite results were found in WB+ (i.e. decrease in HR and increase in RMSSD in CFN and RCV; increase in LF and HF in RCV). When pooling data of the three phases, there were significant correlations between VAS and HR, RMSSD, HF, respectively, in the supine position; the higher the VAS score (i.e., subjective well-being), the higher the RMSSD and HF and the lower the HR. In standing position, HRV parameters were not modified during CFN but RMSSD was correlated to VAS.

Conclusion

Our results suggest that the strict COVID-19 lockdown likely had opposite effects on French population as 20% of participants improved parasympathetic activation (RMSSD, HF) and rated positively this period, whilst 80% showed altered responses and deteriorated well-being. The changes in HRV parameters during and after the lockdown period were in line with subjective well-being responses. The observed recordings may reflect a large variety of responses (anxiety, anticipatory stress, change on physical activity…) beyond the scope of the present study. However, these results confirmed the usefulness of HRV as a non-invasive means for monitoring well-being and health in this population.