bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of Encapsulation Technology: In Vitro Screening of Two Ficus carica L. Genotypes under Different NaCl Concentrations
Authors: Granata Irene , Regni Luca , Micheli Maurizio , Silvestri Cristian , Germanà Maria Antonietta
Published: December 2023

Salinity stress represents an increasing issue for agriculture and has a great negative impact on plant growth and crop production. The selection of genotypes able to tolerate salt stress could be a suitable solution to overcome the problem. In this context, in vitro cultures can represent a tool for identifying the NaCl tolerant genotypes and quickly producing large populations of them. The possibility of exerting selection for tolerance to NaCl by using encapsulation technology was investigated in two genotypes of fig: ‘Houmairi’ and ‘Palazzo’. The effects of five concentrations of NaCl (0, 50, 100, 150 and 200 mM) added to the artificial endosperm were tested on the conversion of synthetic seeds and on the growth of derived shoots/plantlets. Moreover, proline (Pro) and malondialdehyde (MDA), the enzymatic activities of catalase (CAT), guaiacol peroxidase (POD), and EL (Electrolytic Leakage), as well as the chlorophyll content, flavanols, anthocyanins, and Nitrogen Balance Index (NBI) were determined on shoots/plantlet. The obtained results clearly showed that ‘Houmairi’ and ‘Palazzo’ could tolerate salt stress, although a strong difference was found depending on each specific physiological pathway. Indeed, ‘Houmairi’ was revealed to be more tolerant than ‘Palazzo’, with different response mechanisms to salt stress. The use of encapsulated vitro-derived explants proved to be a useful method to validate the selection of genotypes tolerant to salinity stress. Further investigation in the field must validate and confirm the legitimacy of the approach.
Three-Dimensional Imaging in Agriculture: Challenges and Advancements in the Phenotyping of Japanese Quinces in Latvia
Authors: Kaufmane Edīte , Edelmers Edgars , Sudars Kaspars , Namatēvs Ivars , Nikulins Arturs , Strautiņa Sarmīte , Kalniņa Ieva , Peter Astile
Published: December 2023

This study presents an innovative approach to fruit measurement using 3D imaging, focusing on Japanese quince (Chaenomeles japonica) cultivated in Latvia. The research consisted of two phases: manual measurements of fruit parameters (length and width) using a calliper and 3D imaging using an algorithm based on k-nearest neighbors (k-NN), the ingeniously designed “Imaginary Square” method, and object projection analysis. Our results revealed discrepancies between manual measurements and 3D imaging data, highlighting challenges in the precision and accuracy of 3D imaging techniques. The study identified two primary constraints: variability in fruit positioning on the scanning platform and difficulties in distinguishing individual fruits in close proximity. These limitations underscore the need for improved algorithmic capabilities to handle diverse spatial orientations and proximities. Our findings emphasize the importance of refining 3D scanning techniques for better reliability and accuracy in agricultural applications. Enhancements in image processing, depth perception algorithms, and machine learning models are crucial for effective implementation in diverse agricultural scenarios. This research not only contributes to the scientific understanding of 3D imaging in horticulture but also underscores its potential and limitations in advancing sustainable and productive farming practices.
Effect of Photoperiod and Gibberellin on the Bolting and Flowering of Non-Heading Chinese Cabbage
Authors: Liu Shuping , Lu Junyang , Tian Jun , Cao Ping , Li Shuhao , Ge Haicui , Han Mingxuan , Zhong Fenglin
Published: December 2023

Non-heading Chinese cabbage (cabbage) is an essential green leafy vegetable, and bolting and flowering are necessary for reproduction. However, further research is needed to study the effect of photoperiod on the bolting and flowering of cabbage, particularly on the development of the stem. In this study, we performed phenotypic analysis and measured endogenous gibberellin levels in the cabbage. We carried out these experiments under four different photoperiodic treatments, 12 h (light)/12 h (dark), 14 h (light)/10 h (dark), 16 h (light)/8 h (dark), and 18 h (light)/6 h (dark). The results showed that the time of bolting and flowering gradually decreased with increasing light duration. The development of stems was optimal under the 16 h (light)/8 h (dark) photoperiod treatment, and the same result was obtained via cytological observation. In addition, the changes in the endogenous gibberellin3 (GA3) content under different photoperiodic treatments were consistent with the development of stems and peaked at 16 h (light)/8 h (dark). At the same time, qRT-PCR analysis showed that the relative expression of the key gibberellin synthase genes, BcGA3ox2 and BcGA20ox2, exhibited upregulation. When treated with exogenous GA3 and its synthesis inhibitor, paclobutrazol (PAC), exogenous gibberellins significantly promoted bolting; conversely, gibberellin inhibitors suppressed the bolting, flowering, and stem elongation of cabbage. Therefore, the photoperiod may regulate cabbage bolting by regulating endogenous GA3.
UPLC–MS/MS and Gene Expression Research to Distinguish the Colour Differences of Rhododendron liliiflorum H. Lév
Authors: Dai Jin , Wang Xinglin , Meng Xingpan , Zhang Xu , Zhou Qihang , Zhang Zhengdong , Zhang Ximin , Yi Yin , Liu Lunxian , Shen Tie
Published: December 2023

Among ornamental plants, the colour of the petals is an important feature. However, the reason for the colour differences of Rhododendron liliiflorum remains unclear. To reveal the differences in the colour of R. liliiflorum, high-efficiency liquid chromatographic collar (UPLC–MS/MS) technology was used to study the yellow and white parts of R. liliiflorum. A total of 1187 metabolites were identified in R. liliiflorum petals, including 339 flavonoid metabolites. Seventy-eight types of flavonoids in these metabolites were found in the yellow and white parts of R. liliiflorum petals, along with 11 other significantly enriched substances. Combining gene expression-related data with differential metabolite data demonstrated effects of enrichment in the flavanonols (fustin), flavonols (epiafzelechin and afzelechin), and flavanones (pinocembrin) of flavonoid biosynthesis; glyccitin, 6″-O-malonylgenistin, and 6-hydroxydaidzein of isoflavonoid biosynthesis; and anthocyanin biosynthesis of malvidin-3-O-galactoside (primulin), delphinidin-3-O-rutinoside, cyanidin-3-O-glucoside (kuromanin), and cyanidin-3-O-rutinoside (keracyanin), which are potentially the contributing factors responsible for the differences in petal colour in R. liliiflorum. This study establishes a connection between the differential metabolites underlying the color differences in the petals of R. liliiflorum and the gene expression in R. liliiflorum. This will provide a foundation for subsequent research on the regulation of flower color in R. liliiflorum and have profound implications for horticultural applications of R. liliiflorum.
Design, Implementation and Evaluation of an Innovative Pilot Intervention to Improve the Family Quality of Life of Children with Specific Learning Disabilities—A Randomized Controlled Trial
Authors: Pedioti Nektaria , Lioliou Stavroula , Koutra Katerina , Parlalis Stavros , Papadakaki Maria
Published: December 2023

Background: The high prevalence of learning disabilities among children confirm that learning disabilities are surprisingly common. In the absence of routine screening, many children still go undetected with a huge individual and family burden, while at the same time, the findings of existing interventions are conflicting. This study reports on the design, implementation and evaluation of an innovative pilot intervention aiming at improving the quality of life of the family of children with specific learning disabilities. Method: For the purposes of this study, we ran a randomized controlled trial employing an experimental research design with two groups (intervention and control). The study population comprised parents of children with specific learning disabilities. Out of the 71 individuals that were eligible for randomization, 42 were allocated to the intervention, and 29 to the control group. A brief parenting intervention model was employed, aiming at improving parenting skills through a stepwise process. The intervention included four skill building sessions conducted over a period of 6 weeks. “Parenting style” (including three dimensions: “Authoritative”, “Authoritarian” and “Permissive”) and “Family Quality of life” (including five dimensions: “Family Interaction”, “Parenting”, “Emotional Well-being”, “Physical/Material Wellbeing” and “Disability-Related Support”) were employed as the outcome measures of this study. Two validated questionnaires were used to measure the study outcomes: “the Parenting Style Questionnaire” and the “Family Quality of Life Scale (FQOL) Questionnaire”. The questionnaires were applied at the pre- and post-intervention level. Findings: An analysis showed that except for the “permissive parenting style”, the intervention and control group had statistically significant differences in all the “Parenting style” and the “Quality of life” dimensions at the post-intervention level. In the intervention group, none of the study dimensions improved at a statistically significant level at the post-intervention level compared to pre-intervention level. According to the cluster analysis, which re-examined successful vs. unsuccessful cases, the intervention was found to have an effect on the average values of all the “quality of life” and “parenting style” dimensions, except for the “Authoritarian Parenting Style”. Conclusions: The study offers evidence on the dimensions of parenting and quality of life mostly affected by a brief intervention as well as on the feasibility, practicality and acceptance of such interventions in local communities.
Exploiting Synthetic Lethality between Germline BRCA1 Haploinsufficiency and PARP Inhibition in JAK2V617F-Positive Myeloproliferative Neoplasms
Authors: Bermes Max , Rodriguez Maria Jimena , de Toledo Marcelo Augusto Szymanski , Ernst Sabrina , Müller-Newen Gerhard , Brümmendorf Tim Henrik , Chatain Nicolas , Koschmieder Steffen , Baumeister Julian
Published: December 2023

Myeloproliferative neoplasms (MPN) are rare hematologic disorders characterized by clonal hematopoiesis. Familial clustering is observed in a subset of cases, with a notable proportion exhibiting heterozygous germline mutations in DNA double-strand break repair genes (e.g., BRCA1). We investigated the therapeutic potential of targeting BRCA1 haploinsufficiency alongside the JAK2V617F driver mutation. We assessed the efficacy of combining the PARP inhibitor olaparib with interferon-alpha (IFNα) in CRISPR/Cas9-engineered Brca1+/− Jak2V617F-positive 32D cells. Olaparib treatment induced a higher number of DNA double-strand breaks, as demonstrated by γH2AX analysis through Western blot (p = 0.024), flow cytometry (p = 0.013), and confocal microscopy (p = 0.071). RAD51 foci formation was impaired in Brca1+/− cells compared to Brca1+/+ cells, indicating impaired homologous recombination repair due to Brca1 haploinsufficiency. Importantly, olaparib enhanced apoptosis while diminishing cell proliferation and viability in Brca1+/− cells compared to Brca1+/+ cells. These effects were further potentiated by IFNα. Olaparib induced interferon-stimulated genes and increased endogenous production of IFNα in Brca1+/− cells. These responses were abrogated by STING inhibition. In conclusion, our findings suggest that the combination of olaparib and IFNα presents a promising therapeutic strategy for MPN patients by exploiting the synthetic lethality between germline BRCA1 mutations and the JAK2V617F MPN driver mutation.
Probiotic Potential and Safety Assessment of Lactiplantibacillus plantarum cqf-43 and Whole-Genome Sequence Analysis
Authors: Liu Baiheng , Zhong Xiaoxia , Liu Zhiyun , Guan Xiaofeng , Wang Qi , Qi Renli , Zhou Xiaorong , Huang Jinxiu
Published: December 2023

This study reports the whole-genome sequence of Lactiplantibacillus plantarum cqf-43 isolated from healthy sow feces. Based on genomic analysis, we performed a comprehensive safety assessment of strain cqf-43, using both in vitro and in vivo experiments, and explored its probiotic potential. The total genome length measures 3,169,201 bp, boasting a GC content of 44.59%. Through phylogenetic analyses, leveraging both 16S rRNA gene and whole-genome sequences, we confidently categorize strain cqf-43 as a member of Lactiplantibacillus. Genome annotation using Prokka unveiled a total of 3141 genes, encompassing 2990 protein-coding sequences, 71 tRNAs, 16 rRNAs, and 1 tmRNA. Functional annotations derived from COG and KEGG databases highlighted a significant abundance of genes related to metabolism, with a notable emphasis on carbohydrate utilization. The genome also revealed the presence of prophage regions and CRISPR-Cas regions while lacking virulence and toxin genes. Screening for antibiotic resistance genes via the CARD database yielded no detectable transferable resistance genes, effectively eliminating the potential for harmful gene transfer. It is worth highlighting that the virulence factors identified via the VFDB database primarily contribute to bolstering pathogen resilience in hostile environments. This characteristic is particularly advantageous for probiotics. Furthermore, the genome is devoid of menacing genes such as hemolysin, gelatinase, and biogenic amine-producing genes. Our investigation also unveiled the presence of three unannotated secondary metabolite biosynthetic gene clusters, as detected by the online tool antiSMASH, suggesting a great deal of unknown potential for this strain. Rigorous in vitro experiments confirmed tolerance of strain cqf-43 in the intestinal environment, its antimicrobial efficacy, sensitivity to antibiotics, absence of hemolysis and gelatinase activity, and its inability to produce biogenic amines. In addition, a 28-day oral toxicity test showed that the strain cqf-43 did not pose a health hazard in mice, further establishing it as a safe strain.
Genome-Wide Identification, Evolutionary Analysis, and Functional Studies of APX Genes in Melon (Cucuis melo L.)
Authors: Song Jiayan , Zhu Zicheng , Zhang Taifeng , Meng Xiaobing , Zhang Wencheng , Gao Peng
Published: December 2023

The antioxidative enzyme ascorbate peroxidase (APX) exerts a critically important function through scavenging reactive oxygen species (ROS), alleviating oxidative damage in plants, and enhancing their tolerance to salinity. Here, we identified 28 CmAPX genes that display an uneven distribution pattern throughout the 12 chromosomes of the melon genome by carrying out a bioinformatics analysis. Phylogenetic analyses revealed that the CmAPX gene family comprised seven different clades, with each clade of genes exhibiting comparable motifs and structures. We cloned 28 CmAPX genes to infer their encoded protein sequences; we then compared these sequences with proteins encoded by rice APX proteins (OsAPX2), Puccinellia tenuiflora APX proteins (PutAPX) and with pea APX proteins. We found that the CmAPX17, CmAPX24, and CmAPX27 genes in Clade I were closely related, and their structures were highly conserved. CmAPX27 (MELO3C020719.2.1) was found to promote resistance to 150 mM NaCl salt stress, according to quantitative real-time fluorescence PCR. Transcriptome data revealed that CmAPX27 was differentially expressed among tissues, and the observed differences in expression were significant. Virus-induced gene silencing of CmAPX27 significantly decreased salinity tolerance, and CmAPX27 exhibited differential expression in the leaf, stem, and root tissues of melon plants. This finding demonstrates that CmAPX27 exerts a key function in melon’s tolerance to salt stress. Generally, CmAPX27 could be a target in molecular breeding efforts aimed at improving the salt tolerance of melon; further studies of CmAPX27 could unveil novel physiological mechanisms through which antioxidant enzymes mitigate the deleterious effects of ROS stress.
Effect of Low Protein Diets Supplemented with Sodium Butyrate, Medium-Chain Fatty Acids, or n-3 Polyunsaturated Fatty Acids on the Growth Performance, Immune Function, and Microbiome of Weaned Piglets
Authors: Li Wenxue , Lan Tianyi , Ding Qi , Ren Zhongxiang , Tang Zhiru , Tang Qingsong , Peng Xie , Xu Yetong , Sun Zhihong
Published: December 2023

This study aimed to investigate the effects of low-protein (LP) diets supplemented with sodium butyrate (SB), medium-chain fatty acids (MCT), or n-3 polyunsaturated fatty acids (n-3 PUFA) on the growth performance, immune function, and the microbiome of weaned piglets. A total of 120 healthy weaned piglets ((Landrace × Large White × Duroc); 7.93 ± 0.7 kg initial body weight), were randomly divided into five groups. Each group consisted of six replications with four piglets per replication. Dietary treatments included control diet (CON); LP diet (LP); LP + 0.2% SB diet (LP + SB); LP + 0.2% MCT diet (LP + MCT); and LP + PUFA diet (LP + PUFA). The experimental period lasted for 4 weeks. Compared with the CON diet, LP, LP + SB, LP + MCT, and LP + PUFA diets decreased the final weight and average daily gain (ADG) of piglets (p < 0.05). There were lower (p < 0.05) concentrations of IL-8 and higher (p < 0.05) Glutathione peroxidase (GSH-Px) activity in the plasma of piglets fed with LP + SB, LP + MCT, and LP + PUFA diets than those fed with the LP diet. The piglets in the LP + SB and LP + PUFA groups had lower IKK-alpha (IKKa) mRNA expression in the colonic mucosa compared with those in the CON and LP groups (p < 0.05). The mRNA expression of TLR4 in the colonic mucosa of piglets in the LP + SB, LP + MCT, and LP + PUFA groups was decreased when compared with the CON and LP groups (p < 0.05). The LP + MCT diets increased the gene expression of nuclear factor erythroid 2-related factor 2 (Nrf2) in the colonic mucosa of piglets compared with CON, LP, and LP + SB diets (p < 0.05). The abundance of Erysipelotrichaceae in the colonic microbiome of piglets in the LP group was higher than that in the other four groups (p < 0.05). Collectively, this study showed that LP diets supplemented with SB, MCT, or n-3 PUFA reduced plasma inflammatory factor levels, increased plasma GSH-Px activity, and declined mRNA expression of TLR4 and IKKa in the colonic epithelium, whereas it reduced the abundance of Erysipelotrichaceae in the colon of piglets.
Involvement of Type 10 17β-Hydroxysteroid Dehydrogenase in the Pathogenesis of Infantile Neurodegeneration and Alzheimer’s Disease
Authors: He Xue-Ying , Frackowiak Jannusz , Dobkin Carl , Brown William Ted , Yang Song-Yu
Published: December 2023

Type 10 17β-hydroxysteroid dehydrogenase (17β-HSD10) is the HSD17B10 gene product playing an appreciable role in cognitive functions. It is the main hub of exercise-upregulated mitochondrial proteins and is involved in a variety of metabolic pathways including neurosteroid metabolism to regulate allopregnanolone homeostasis. Deacetylation of 17β-HSD10 by sirtuins helps regulate its catalytic activities. 17β-HSD10 may also play a critical role in the control of mitochondrial structure, morphology and dynamics by acting as a member of the Parkin/PINK1 pathway, and by binding to cyclophilin D to open mitochondrial permeability pore. 17β-HSD10 also serves as a component of RNase P necessary for mitochondrial tRNA maturation. This dehydrogenase can bind with the Aβ peptide thereby enhancing neurotoxicity to brain cells. Even in the absence of Aβ, its quantitative and qualitative variations can result in neurodegeneration. Since elevated levels of 17β-HSD10 were found in brain cells of Alzheimer’s disease (AD) patients and mouse AD models, it is considered to be a key factor in AD pathogenesis. Since data underlying Aβ-binding-alcohol dehydrogenase (ABAD) were not secured from reported experiments, ABAD appears to be a fabricated alternative term for the HSD17B10 gene product. Results of this study would encourage researchers to solve the question why elevated levels of 17β-HSD10 are present in brains of AD patients and mouse AD models. Searching specific inhibitors of 17β-HSD10 may find candidates to reduce senile neurodegeneration and open new approaches for the treatment of AD.
Modification of Graphite/SiO2 Film Electrodes with Hybrid Organic–Inorganic Perovskites for the Detection of Vasoconstrictor Bisartan 4-Butyl-Ν,Ν-bis{[2-(2H-tetrazol-5-yl)biphenyl-4-yl]methyl}imidazolium Bromide
Authors: Papathanidis Georgios , Ioannou Anna , Spyrou Alexandros , Mandrapylia Aggeliki , Kelaidonis Konstantinos , Matsoukas John , Koutselas Ioannis , Topoglidis Emmanuel
Published: December 2023

In the present work, a hybrid organic–inorganic semiconductor (HOIS) has been used to modify the surface of a graphite paste/silica (G–SiO2) film electrode on a conducting glass substrate to fabricate a promising, sensitive voltammetric sensor for the vasoconstrictor bisartan BV6, which could possibly treat hypertension and COVID-19. The HOIS exhibits exceptional optoelectronic properties with promising applications not only in light-emitting diodes, lasers, or photovoltaics but also for the development of voltammetric sensors due to the ability of the immobilized HOIS lattice to interact with ions. This study involves the synthesis and characterization of an HOIS and its attachment on the surface of a G–SiO2 film electrode in order to develop a nanocomposite, simple, sensitive with a fast-response, low-cost voltammetric sensor for BV6. The modified HOIS electrode was characterized using X-ray diffraction, scanning electron microscopy, and optical and photoluminescence spectroscopy, and its electrochemical behavior was examined using cyclic voltammetry. Under optimal conditions, the modified G–SiO2 film electrode exhibited a higher electrocatalytic activity towards the oxidation of BV6 compared to a bare graphite paste electrode. The results showed that the peak current was proportional to BV6 concentration with a linear response range from 0 to 65 × 10−6 (coefficient of determination, 0.9767) and with a low detection limit of 1.5 × 10−6 M (S/N = 3), estimated based on the area under a voltammogram, while it was 3.5 × 10−6 for peak-based analysis. The sensor demonstrated good stability and reproducibility and was found to be appropriate for the determination of drug compounds such as BV6.
Response of Spider and Epigaeic Beetle Assemblages to Overwinter Planting Regimes and Surrounding Landscape Compositions
Authors: Chong Hainan , Zhu Yulin , Lai Qian , Wu Song , Jiang Ting , Zhang Dandan , Xiao Haijun
Published: December 2023

The rotation patterns of summer rice–winter oil seed rape and summer rice–winter fallow are the main planting regimes in the rice ecosystem in southern China. However, the impact of local rotation patterns and landscape factors on the overwintering conservation of predators in spider and epigaeic beetle assemblages remains poorly understood. Here, we investigate the diversity and density of spiders and beetles over two consecutive winters (2019/2020 and 2020/2021), focusing on the impact of two rotation patterns (rice–fallow and rice–oilseed rape) and surrounding landscape compositions on predator diversity. The main findings of our research were that spiders were more abundant and had a higher activity density in the fallow rice fields (FRs) compared to the oilseed rape fields (OSRs), whereas ground beetles exhibited the opposite pattern. Specifically, fallow rice fields supported small and ballooning spiders (e.g., dominant spider: Ummeliata insecticeps), while OSRs supported larger ground beetles (e.g., dominant beetles: Agonum chalcomus and Pterostichus liodactylus). Moreover, the composition of spider assemblages were impacted by semi-natural habitats (SNHs) during overwintering, while ground beetle assemblages were influenced by overwinter planting patterns. Overall, our results suggest that different planting regimes and preserving semi-natural habitats are a strategic way to enhance species diversity and functional diversity of ground predators. It is, therefore, recommended that to conserve and improve predator diversity during overwintering, land managers and farmers should aim to maintain diverse planting regimes and conserve local semi-natural habitats.
Monitoring and Prediction of Siberian Silk Moth Dendrolimus sibiricus Tschetv. (Lepidoptera: Lasiocampidae) Outbreaks Using Remote Sensing Techniques
Authors: Soukhovolsky Vladislav , Kovalev Anton , Goroshko Andrey , Ivanova Yulia , Tarasova Olga
Published: December 2023

The feasibility of risk assessment of a Siberian silk moth (Dendrolimus sibiricus Tschetv.) outbreak was analyzed by means of landscape and weather characteristics and tree condition parameters. Difficulties in detecting forest pest outbreaks (especially in Siberian conditions) are associated with the inability to conduct regular ground surveillance in taiga territories, which generally occupy more than 2 million km2. Our analysis of characteristics of Siberian silk moth outbreak zones under mountainous taiga conditions showed that it is possible to distinguish an altitudinal belt between 400 and 800 m above sea level where an outbreak develops and trees are damaged. It was found that to assess the resistance of forest stands to pest attacks, researchers can employ new parameters: namely, characteristics of a response of remote sensing variables to changes in land surface temperature. Using these parameters, it is possible to identify in advance (2–3 years before an outbreak) forest stands that are not resistant to the pest. Thus, field studies in difficult-to-access taiga forests are not needed to determine these parameters, and hence the task of monitoring outbreaks of forest insects is simplified substantially.
Using Functionally Redundant Inertial Measurement Units to Increase Reliability and Ensure Fault Tolerance
Authors: Kuznetsov Ivan M. , Veremeenko Konstantin K. , Zharkov Maxim V. , Pronkin Andrey N.
Published: December 2023

This paper aims to assess the possibility of using functionally redundant inertial units to solve problems of increasing reliability and ensuring the fault tolerance of the various classes and purposes of aircraft navigation systems. We present the results of studying failure detection methods to improve the accuracy and reliability of a strapdown functionally redundant inertial unit. The resulting structural redundancy of the strapdown inertial measurement unit is designed to increase the fault tolerance and accuracy of strapdown inertial navigation systems. The methods for detecting sensor failures in functionally redundant inertial units are based on the use of the equations of functionally redundant inertial unit compliance to nominal requirements for the accuracy of measuring the input action vector. To describe the methods for detecting and eliminating failed sensor and algorithm designs based on them, we gave the mathematical models of the measurement vector of functionally redundant inertial units concerning the measured vector and the error identification condition, including the residual of the matching equations with the size due to the level of redundancy, determining the total number of matching equations. The main criterion for determining a failed sensor is non-compliance with the nominal value of the residual included in a certain number of matching equations of the information received from such meters. The developed algorithms are examined using simulation methods. The study of the selected structure of the functionally redundant inertial units shows that the proposed approaches are efficient. Also, we manage to identify the main characteristics of the algorithms for detecting sensor failures that are structurally a part of the functionally redundant inertial units.
Ultrasound-Guided Bilateral Erector Spinae Plane Block vs. Ultrasound-Guided Bilateral Posterior Quadratus Lumborum Block for Postoperative Analgesia after Caesarean Section: An Observational Closed Mixed Cohort Study
Authors: Zanfini Bruno A. , Di Muro Mariangela , Biancone Matteo , Catarci Stefano , Piersanti Alessandra , Frassanito Luciano , Ciancia Mariano , Toni Flavia , Santantonio Maria Teresa , Draisci Gaetano
Published: December 2023

ESP block (ESPB) and posterior Quadratus Lumborum Block (pQLB) have been proposed as opioid-sparing techniques for the management of pain after abdominal surgery. Between December 2021 and October 2022, we conducted a retrospective comparative study at the delivery suite of Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy, to compare the efficacy of ESPB and pQLB in preventing postoperative pain after an elective caesarean section (CS). The primary outcome was total morphine consumption in the first 24 h. Secondary outcomes were time to first opioid request; Numerical Pain Rating Scale (NPRS) at 0, 2, 6, 12 and 24 h; vital signs; adverse events. Fifty-two women were included. The total cumulative dose of morphine was not significantly different between the two groups of patients (p = 0.897). Time to first dose of morphine, NPRS values and haemodynamic parameters were not statistically different between the two groups. NPRS values significantly increased (p < 0.001) at the different time intervals considered. The need for rescue doses of morphine was lower in the ESPB group compared to the pQLB group (hazard ratio of 0.51, 95% CI (0.27 to 0.95), p = 0.030). No adverse event was reported. ESPB seems to be as effective as pQLB in providing analgesia after CS.
Percutaneous Ureteral Stent Insertion in Patients with Benign Uretero-Ileal Anastomosis Strictures Who Underwent Radical Cystectomy: Assessing Risk Factors for Stent Patency
Authors: Kim Yebin , Oh Chang Hoon , Choi Sang Lim , Kim Sungwon
Published: December 2023

We aimed to investigate the risk factors of early double-J ureteral stent (DJUS) dysfunction rates and the long-term patency of DJUSs inserted via a percutaneous approach in patients with benign uretero-ileal anastomosis stricture (UIAS) who had undergone radical cystectomy. In this retrospective study, 63 DJUS placements were placed via a percutaneous nephrostomy tract in 42 consecutive patients between May 2020 and March 2023. The technical success rate was 100% in all patients without major complications. The early dysfunction rate and long-term patency rate were 38.1% (24/63) and 84.2% (32/38), respectively. The blood clot retention grade, balloon dilatation, and length of the ureteral stricture exhibited a significant correlation with early DJUS dysfunction (blood clot retention grade: odds ratio (OR) 6.922 in grade two, p = 0.009; balloon dilatation: OR 0.186, p = 0.017; length of ureteral stricture: OR 8.715, p = 0.035 in moderate stenosis, and 7.646, p = 0.028 in severe stenosis). A multivariate Cox’s proportional hazard analysis revealed that blood clot retention grade and length of ureteral stricture were independent predictors of long-term DJUS patency. Percutaneous insertion of the DJUSs was safe and effective in patients with benign UIAS.
Face Mask and Tear Film Stability: A Pilot Study of the Objective Measurement of Tear Break-Up Time
Authors: Mohamed-Noriega Karim , Charles-Cantu David E. , Mohamed-Noriega Jibran , Velasco-Sepúlveda Braulio H. , Morales-Wong Fernando , Villarreal-Méndez Gerardo , Mohamed-Hamsho Jesús
Published: December 2023

(1) Background: Mask-associated dry eye (MADE) has been associated with increased dry eye symptoms, apparently due to reduced tear break-up time (TBUT). This study aimed to determine the short-term impact of surgical face mask (FM) on tear film stability by measuring non-invasive tear break-up time (NIBUT). (2) Methods: Twenty-six healthy participants had NIBUT evaluated without FM, with surgical FM and with a surgical FM secured to the skin with adhesive tape (TFM). NIBUT-first was measured with Keratograph 5M (K5M, Oculus, Wetzlar, Germany). Each participant had NIBUT measured in four sessions on four consecutive days. Session 1: without FM vs. with FM. Session 2: with FM vs. without FM. Session 3: without FM vs. with TFM. Session 4: with TFM vs. without FM (3). The time between each measured setting was 2 min. Results: The mean ± SD NIBUT without FM was 8.9 ± 3.7, with FM 10.2 ± 4.1, and with TFM 8.4 ± 3.8 s. No significant differences were observed in NIBUT in any of the evaluated settings: without FM vs. with FM (p = 0.247), without FM vs. with TFM (p = 0.915), and with FM vs. with TFM (p = 0.11). (4) Conclusions: This study did not find a significant short-term effect of FM on NIBUT. Other variables or longer periods of exposure might trigger the symptoms and ocular surface alterations in MADE.
Immune Checkpoint Inhibitors-Associated Myocarditis: Diagnosis, Treatment and Current Status on Rechallenge
Authors: Frascaro Federica , Bianchi Nicola , Sanguettoli Federico , Marchini Federico , Meossi Sofia , Zanarelli Luca , Tonet Elisabetta , Serenelli Matteo , Guardigli Gabriele , Campo Gianluca , Calabrò Luana , Pavasini Rita
Published: December 2023

Immune checkpoint molecules like cytotoxic T-lymphocyte antigen 4 (CTLA-4), programmed cell death 1 (PD-1) or its ligand, programmed cell death ligand 1 (PD-L1), play a critical role in regulating the immune response, and immune checkpoint inhibitors (ICIs) targeting these checkpoints have shown clinical efficacy in cancer treatment; however, their use is associated with immune-related adverse events (irAEs), including cardiac complications. The prevalence of cardiac irAEs, particularly myocarditis, is relatively low, but they can become a severe and potentially life-threatening condition, usually occurring shortly after initiating ICI treatment; moreover, diagnosing ICI-related myocarditis can be challenging. Diagnostic tools include serum cardiac biomarkers, electrocardiography (ECG), echocardiography, cardiac magnetic resonance (CMR) and endomyocardial biopsy (EMB). The treatment of ICI-induced myocarditis involves high-dose corticosteroids, which have been shown to reduce the risk of major adverse cardiac events (MACE). In refractory cases, second-line immunosuppressive drugs may be considered, although their effectiveness is based on limited data. The mortality rates of ICI-induced myocarditis, particularly in severe cases, are high (38–46%). Therapy rechallenge after myocarditis is associated with a risk of recurrence and severe complications. The decision to rechallenge should be made on a case-by-case basis, involving a multidisciplinary team of cardiologists and oncologists. Further research and guidance are needed to optimize the management of cancer patients who have experienced such complications, evaluating the risks and benefits of therapy rechallenge. The purpose of this review is to summarize the available evidence on cardiovascular complications from ICI therapy, with a particular focus on myocarditis and, specifically, the rechallenge of immunotherapy after a cardiac adverse event.
SCAI Staging Application for Acute Myocardial Infarction-Related Cardiogenic Shock at a Single-Center Russian Registry
Authors: Ryabov Vyacheslav V. , Panteleev Oleg O. , Kercheva Maria A. , Gorokhovsky Alexei A. , Syrkina Anna G. , Margolis Natalia Y.
Published: December 2023

Aim: To access the features of the course of myocardial infarction (MI) in patients with different stages of MI complicated by cardiogenic shock (MI CS) according to the SCAI scale. Methods: We retrospectively described the portrait of CS MI (n = 117) at different stages of SCAI from the hospital MI registry (n = 1253). Results: Hospital mortality increased from stage to stage (p ≤ 0.001). Significant differences in biochemical parameters were found both for indicators characterizing intensive care measures, such as the presence of mechanical lung ventilation or an intra-aortic balloon pump, and for indicators of organ hypoperfusion such as lactate level, pHv (7.39 (7.36; 7.44) at stage A–B; 7.14 (7.06; 7.18) at stage E), creatinine, and glomerular filtration rate. Parameters related to MI characteristics, such as instrumental and laboratory data, anamnesis of ischemia, and performed treatment, did not differ between groups. Polynomial logistic regression showed that lactate level, mechanical ventilation, and monocyte count upon admission (1.15 (0.96; 1.23) at stage A–B; 0.78 (0.49; 0.94) at stage E, p = 0.005) correlated with CS severity. Conclusion: The characteristics of MI at different stages of SCAI do not have differences and do not determine the severity of shock. We revealed a high discriminatory potential of the pH level in predicting refractory shock. The value of monocytes at admission may be a promising predictor of the severity of MI CS. The question of the causes of heterogeneity of MI CS, taking into account the homogeneity of MI characteristics, remains open and promising.
Classification of Peripheral Blood Leukocyte Phenotypes and Serum Cytokines in Vogt–Koyanagi–Harada Disease before and after Glucocorticoid Therapy
Authors: Sato Tomohito , Taniguchi Nanae , Nishio Yoshiaki , Ito Masataka , Takeuchi Masaru
Published: December 2023

Vogt–Koyanagi–Harada disease (VKH) is an autoimmune disease, and glucocorticoid therapy (GC) is widely used for VKH. We provided a profile of leukocyte populations and serum cytokines in VKH patients under GC. A prospective observational study was conducted on three treatment-naïve VKH patients. Peripheral blood samples were collected from the patients before GC (VKH-acute) and after 6 months (VKH-remission), and healthy individuals were used as controls. Proportions of 37-type leukocytes and levels of 27-kind cytokines were measured by mass cytometry and multiplex bead analysis. Property similarity was analyzed using hierarchical cluster analysis. The leukocytes and cytokines were broadly classified into four and three clusters: (1) a cluster with high intensity in VKH-acute consisting of B cells, Th2-like, Th17-like, basophils, and IL-7 and IP-10; (2) a cluster with high intensity in VKH-remission composed of monocytes, neutrophils, IL-4, and TNFα; in leukocytes, (3) a cluster with low intensity in VKH-acute and -remission consisting of CD8+ T cells, Th1-like, and NKT cells; (4) a cluster with low intensity in VKH-remission composed of NK cells, Tregs, and DCs; and in cytokines, (5) a cluster with high intensities in VKH-acute and -remission comprising G-CSF, MCP-1, eotaxin, and IL-17A. These findings suggest that inflammatory composition in blood during the acute phase of VKH represents complex hyperimmune responses dominantly driven by Th and B cells.
Effects of High-Intensity Interval Training Using the 3/7 Resistance Training Method on Metabolic Stress in People with Heart Failure and Coronary Artery Disease: A Randomized Cross-Over Study
Authors: Gillet Alexis , Forton Kevin , Lamotte Michel , Macera Francesca , Roussoulières Ana , Louis Pauline , Ibrahim Malko , Dewachter Céline , van de Borne Philippe , Deboeck Gaël
Published: December 2023

The 3/7 resistance training (RT) method involves performing sets with increasing numbers of repetitions, and shorter rest periods than the 3x9 method. Therefore, it could induce more metabolic stress in people with heart failure with reduced ejection fraction (HFrEF) or coronary artery disease (CAD). This randomized cross-over study tested this hypothesis. Eleven individuals with HFrEF and thirteen with CAD performed high-intensity interval training (HIIT) for 30 min, followed by 3x9 or 3/7 RT according to group allocation. pH, HCO3−, lactate, and growth hormone were measured at baseline, after HIIT, and after RT. pH and HCO3− decreased, and lactate increased after both RT methods. In the CAD group, lactate increased more (6.99 ± 2.37 vs. 9.20 ± 3.57 mmol/L, p = 0.025), pH tended to decrease more (7.29 ± 0.06 vs. 7.33 ± 0.04, p = 0.060), and HCO3− decreased more (18.6 ± 3.1 vs. 21.1 ± 2.5 mmol/L, p = 0.004) after 3/7 than 3x9 RT. In the HFrEF group, lactate, pH, and HCO3− concentrations did not differ between RT methods (all p > 0.248). RT did not increase growth hormone in either patient group. In conclusion, the 3/7 RT method induced more metabolic stress than the 3x9 method in people with CAD but not HFrEF.
Dolutegravir/Lamivudine versus Tenofovir Alafenamide/Emtricitabine/Bictegravir as a Switch Strategy in a Real-Life Cohort of Virogically Suppressed People Living with HIV
Authors: De Socio Giuseppe Vittorio , Tordi Sara , Altobelli Debora , Gidari Anna , Zoffoli Anastasia , Francisci Daniela
Published: December 2023

Background: The aim of the study is to evaluate the effectiveness, safety, and tolerability of a two-drug regimen (2-DR) dolutegravir/lamivudine (DTG/3TC) versus a three-drug regimen (3-DR) tenofovir alafenamide/emtricitabine/bictegravir (TAF/FTC/BIC) in a real-life cohort of HIV-1 virologically suppressed treatment-experienced (TE) people living with HIV (PLWH). Methods: This was a single-center, retrospective, observational study analyzing adult TE PLWH who started the 2-DR or 3-DR between January 2018 and January 2023. All PLWH with a viral load (VL) <50 copies/mL at the time of switching, and a follow-up of more than 6 months or interruption of treatment at any time, were included. Results: A total of 324 PLWH were included; of these, 110 (34%) were on the 2-DR and 214 (66%) were on the 3-DR. Most patients remained on therapy in both groups (93.6% 2-DR versus 90.2% 3-DR) and, at the last control, 99.1% achieved VL < 50 copies/mL with the 2-DR versus 97.2% with the 3-DR (p = 0.260). No virological failures occurred in either group. Adverse events occurred in a few cases: four (3.6%) in the 2-DR group and five (2.3%) in the 3-DR group (p = 0.500). The median follow-up-time was 19.6 months for the 2-DR and 27.5 months for the 3-DR. Conclusion: Our study shows a similar effectiveness and safety profile in virologically suppressed PLWH switching to DTG/3TC or TAF/FTC/BIC.
Progress in Studies of Disentangled Polymers and Composites
Authors: Pawlak Andrzej , Krajenta Justyna
Published: December 2023

Macromolecule entanglements are common in polymers. The first part of this review describes their influence on the properties of entangled polymers. Then, methods for reducing the entanglement density of macromolecule chains are discussed. It has been shown that research on partially disentangled polymers has provided a lot of new information about the relationship between the entangled state and properties of polymers. This research concerns, among others, mechanical and thermal properties and the crystallization process. A special disentangled polymer case, ultra-high-molecular-weight polyethylene, is also discussed. The results of research on polymer composites in which macromolecules were disentangled via processing and composites were produced using already disentangled polymers are presented in particular detail. It has been indicated that such composites and blends of disentangled polymers are promising and will probably be intensively researched in the near future.
Effect of Bioactive Glasses and Basic Fibroblast Growth Factor on Dental Pulp Cells
Authors: Washio Ayako , Kérourédan Olivia , Tabata Yasuhiko , Kokabu Shoichiro , Kitamura Chiaki
Published: December 2023

Ideal regeneration of hard tissue and dental pulp has been reported with the use of a combination of bioactive glass and basic fibroblast growth factor (bFGF). However, no previous study has investigated the molecular mechanisms underlying the processes induced by this combination in dental pulp cells. This study aimed to examine the cellular phenotype and transcriptional changes induced by the combination of bioactive glass solution (BG) and bFGF in dental pulp cells using phase-contrast microscopy, a cell counting kit-8 assay, alkaline phosphatase staining, and RNA sequence analysis. bFGF induced elongation of the cell process and increased the number of cells. Whereas BG did not increase ALP activity, it induced extracellular matrix-related genes in the dental pulp. In addition, the combination of BG and bFGF induces gliogenesis-related genes in the nervous system. This is to say, bFGF increased the viability of dental pulp cells, bioactive glass induced odontogenesis, and a dual stimulation with bioactive glass and bFGF induced the wound healing of the nerve system in the dental pulp. Taken together, bioactive glass and bFGF may be useful for the regeneration of the dentin-pulp complex.
Critical Thinking: The ARDESOS-DIAPROVE Program in Dialogue with the Inference to the Best and Only Explanation
Authors: Guamanga Miguel H. , González Fabián A. , Saiz Carlos , Rivas Silvia F.
Published: December 2023

In our daily lives, we are often faced with the need to explain various phenomena, but we do not always select the most accurate explanation. For example, let us consider a “toxic” relationship with physical and psychological abuse, where one of the partners is reluctant to end it. Explanations for this situation can range from emotional or economic dependency to irrational hypotheses such as witchcraft. Surprisingly, some people may turn to the latter explanation and consequently seek ineffective solutions, such as visiting a witch doctor instead of a psychologist. This choice of an inappropriate explanation can lead to actions that are not only ineffective but potentially harmful. This example underscores the importance of inference to the best explanation (IBE) in everyday decision making. IBE involves selecting the hypothesis that would best explain the available body of data or evidence, a process that is crucial to making sound decisions but is also vulnerable to bias and errors of judgment. Within this context, the purpose of our article is to explore how the IBE process and the selection of appropriate explanations impact decision making and problem solving in real life. To this end, we systematically analyze the role of IBE in the ARDESOS-DIAPROVE program, evaluating how this approach can enhance the teaching and practice of critical thinking.
Testing Galileo High-Accuracy Service (HAS) in Marine Operations
Authors: Pintor Pedro , Lopez-Martinez Manuel , Gonzalez Emilio , Safar Jan , Boyle Ronan
Published: December 2023

Global Navigation Satellite System (GNSS) technology supports all phases of maritime navigation and serves as an integral component of the Automatic Identification System (AIS) and, by extension, Vessel Traffic Service (VTS) systems. However, the accuracy of standalone GNSS is often insufficient for specific operations. To address this limitation, various regional and local-area solutions have been developed, such as Differential GNSS (DGNSS), Satellite Based Augmentation Service (SBAS) and Real Time Kinematic (RTK) techniques. A notable development in this field is the recent introduction of the Galileo High-Accuracy Service (HAS), which saw its initial service declared operational by the European Commission (EC) on 24 January 2023. Galileo HAS provides high-accuracy Precise Point Positioning (PPP) corrections (orbits, clocks and signal biases) for Galileo and GPS, enhancing real-time positioning performance at no additional cost to users. This article presents the results of the first Galileo HAS testing campaign conducted at sea using a buoy-laying vessel temporarily equipped with a Galileo HAS User Terminal. The results presented in this Article include accuracy and position availability performance achieved using the Galileo HAS User Terminal. The article also highlights challenges posed by high-power radio-frequency interference, which likely originated from the Long-Range Identification and Tracking (LRIT) system antenna on board the vessel. Furthermore, the article provides additional assessments for different phases of navigation, demonstrating better performance in slow-motion scenarios, particularly relevant to mooring and pilotage applications. In these scenarios, values for horizontal accuracy reached 0.22 m 95% and 0.13 m 68% after removing interference periods. These results are in line with the expectations outlined in the Galileo HAS Service Definition Document (SDD).