bluuebunny/crossref_metadata_2025 huggingface dataset:
Isolation and Expression of Transcription Factors Involved in Somatic Embryo Development by Transcriptome Analysis of Embryogenic Callus of Thuja koraiensis
Authors: Ahn Chang Ho , Han Jung Yeon , Park Hyeong Soo , Yoon Hyun Won , Shin Jung Won , Seo Jeong Min , Lee Hana , Kim Yeoung Ryul , Baek Saeng Geul , Nam Jae Ik , Kim Jung Min , Choi Yong Eui
Published: January 2023

Thuja koraiensis Nakai (Cupressaceae) is an endangered and ecologically important conifer endemic to Korea. Previously, we established a protocol for micropropagation in T. koraiensis, which involved somatic embryogenesis from embryogenic callus of T. koraiensis. However, the molecular mechanisms underlying somatic embryogenesis remain unclear. Herein, we performed transcriptomic analysis to identify somatic embryogenesis-related genes of T. koraiensis via Illumina RNA sequencing. We conducted de novo transcriptome assembly using a Trinity assembler, which produced 274,077 transcript contigs clustered into 205,843 transcripts (unigenes), with an average length of 825 base pairs. Of all the unigenes, 14.69%, 18.62%, and 7.4% had homologs in the Gene Ontology, NCBI Non-redundant Protein, and NCBI Nucleotide databases, respectively. Among these mRNA sequences, expression of putative embryogenesis-associated transcription factors, namely BABYBOOM (BBM), WUSCHEL-RELATED HOMEOBOX (WOX), and SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK), was analyzed during somatic embryogenesis. RT-PCR analysis revealed that TkBBM, TkWOX, and TkSERK were highly expressed in embryogenic callus and seedling roots, whereas their expression was reduced in seedling leaves. Our findings provide new insights into the roles of BBM, WOX, and SERK in somatic embryogenesis. Our results may serve as a reference for comparative transcriptome analysis in related species and further aid functional genomics studies in conifers.
Morpho-Physiological and Transcriptional Regulation of Root System under Saline Conditions in Nymphaea Plants
Authors: Chen Shaozhou , Du Fengfeng , Huang Qianhao , Gao Xiaojing , Zhang Zhiyuan , Cui Jian , Chang Yajun , Liu Xiaojing , Yao Dongrui
Published: January 2023

Water lilies (Nymphaea L.) are ancient angiosperms that can be cultivated in both fresh and brackish water. Water lily plants have adapted morphologically and physiologically to the aqueous environment. Nonetheless, little is known about the regulatory mechanisms that enable water lily to acclimate to saline conditions, restricting its production and distribution. To illustrate the role of roots in water lily salinity tolerance, we investigated the adaptive regulation of the water lily root system under high salinity. Aspects of its root architecture, including root length, surface area, volume, and tip number, were significantly reduced by salt stress. Transcriptome sequencing showed that 120 genes were upregulated and 1214 genes were downregulated under salt stress. The differentially expressed genes were mainly enriched in oxidoreductase activity, structural molecule activity, and transmembrane transporter activity. Most ion transporter genes were downregulated, suggesting that water lily may partially close ion channels and/or transporters to avoid excessive ion accumulation or ion imbalance under long-term salt stress. Genes related to NO3− transport were both up- and downregulated, whereas genes related to ammonium transport were uniformly downregulated, suggesting that transcriptional changes may play a role in balancing nitrogen metabolism under long-term saline conditions. The roots showed relatively high concentrations of Na+ and had the ability to hyper-accumulate Na+ under salt stress. These findings provide insight into the regulatory mechanisms that enable water lily roots to tolerate salinity and lay a foundation for the breeding of salt-tolerant cultivars.
Evaluation of Multispectral Data Acquired from UAV Platform in Olive Orchard
Authors: Catania Pietro , Roma Eliseo , Orlando Santo , Vallone Mariangela
Published: January 2023

Precision agriculture is a management strategy to improve resource efficiency, production, quality, profitability and sustainability of the crops. In recent years, olive tree management is increasingly focused on determining the correct health status of the plants in order to distribute the main resource using different technologies. In the olive grove, the focus is often on the use of multispectral information from UAVs (Unmanned Aerial Vehicle), but it is not known how important spectral and biometric information actually is for the agronomic management of the olive grove. The aim of this study was to investigate the ability of multispectral data acquired from a UAV platform to predict nutritional status, biometric characteristics, vegetative condition and production of olive orchard as tool to DSS. Data were collected on vegetative characteristics closely related to vigour such as trunk cross-sectional area (TCSA), Nitrogen concentration of the leaves, canopy area and canopy volume. The production was collected for each plant to create an accurate yield map. The flight was carried out with a UAV equipped with a multispectral camera, at an altitude of 50 m and with RTK correction. The flight made it possible to determine the biometric condition and the spectral features through the normalized difference vegetation index (NDVI). The NDVI map allowed to determine the canopy area. The Structure for Motion (SfM) algorithm allow to determine the 3D canopy volume. The experiment showed that the NDVI was able to determine with high accuracy the vegetative characteristic as canopy area (r = 0.87 ***), TCSA (r = 0.58 ***) and production (r = 0.63 ***). The vegetative parameters are closely correlated with the production, especially the canopy area (r = 0.75 ***). Data clustering showed that the production of individual plants is closely dependent on leaf nitrogen concentration and vigour status.
State of the Oral Implantology Practice in Spain during the COVID-19 Pandemic: A Cross-Sectional Survey
Authors: Salgado-Peralvo Ángel-Orión , Fernández-Cerero Daniel , Garcia-Sanchez Alvaro , Kewalramani Naresh , Velasco-Ortega Eugenio , Jiménez-Guerra Álvaro , Ortiz-García Iván , Moreno-Muñoz Jesús , Núñez-Márquez Enrique , López-López José , Monsalve-Guil Loreto
Published: January 2023

At the beginning of the COVID-19 pandemic, strict measures of confinement and social distancing were taken. Dentists were considered essential personnel and their activity was restricted to emergency treatment. The present observational study aims to determine the situation of oral implantology practice in Spain during the initial period of the COVID-19 pandemic. This is a cross-sectional observational study based on the STROBE (Strengthening the Reporting of Observational Studies in Epidemiology) guidelines. An electronic survey consisting of three blocks of questions was sent to all members of the Spanish Society of Implants. The data were analyzed using descriptive analysis. A total of 237 participants (14.3%) responded to the questionnaire. The majority of participants (60.8%) only attended emergencies during the first 9 months of the pandemic. Despite this, 77.2% reported having performed dental implant surgeries and 75.5% indicated that they performed non-essential treatments. The activity was fully recovered by 64.1% of the surveyed dentists. The majority of dentists (90.7%) considered that sufficient/adequate preventive measures were carried out at their workplace, which possibly contributed to the fact that 49.3% were not afraid of becoming infected. This concern was significantly and directly proportionally associated with the age of the surveyed dentists. The oral implant practice was affected to a greater extent during the first 9 months of the pandemic, especially in urban areas, with a greater impact on the workload of professionals with less specialised training in oral implantology.
School-Based Interventions to Support Healthy Indoor and Outdoor Environments for Children: A Systematic Review
Authors: Fernandes Amanda , Ubalde-López Mònica , Yang Tiffany C. , McEachan Rosemary R. C. , Rashid Rukhsana , Maitre Léa , Nieuwenhuijsen Mark J. , Vrijheid Martine
Published: January 2023

Environmental exposures are associated with children’s health. Schools are often urban exposure ‘hotspots’ for pollution, noise, lack of green space and un-walkable built environments. The aim of this systematic review was to explore the impact of school-based interventions on the modification of indoor and outdoor stressors related to the built and natural environment on children’s exposure and health. A systematic review of seven databases was performed. We included quantitative studies on children aged 5–12, which reported intervention delivered within school settings aimed at addressing key environmental exposures including air pollution, green spaces, traffic noise or active travel; and reported physical and mental health, physical activity or active travel behavior. The quality of studies was assessed and interventions were described using a standardized framework. A narrative synthesis approach was used to describe the findings. Thirty-nine papers were included on three main intervention types: improve indoor air quality by the increase of ventilation rates in classrooms; increase children’s green time or greening schools, and multicomponent interventions to increase active travel to school by changes in pedestrian facilities. No eligible intervention to reduce traffic noise at school was found. Increasing ventilation rates improved short-term indoor air quality in classrooms, but the effect on cognitive performance was inconsistent. Greening schools and increasing children’s green time have consistent positive effects on cognition and physical activity, but not in behavior. Multi-component interventions can increase walking and cycling after three years. Overall, the studies were rated as having poor quality owing to weak study designs. We found modest evidence that school-based built and natural environment interventions can improve children’s exposure and health.
Influence of Interocular Differences and Alcohol Consumption on Binocular Visual Performance
Authors: Martino Francesco , Castro-Torres José , Casares-López Miriam , Ortiz-Peregrina Sonia , Granados-Delgado Pilar , Jiménez José
Published: January 2023

The purpose of this study was to assess the influence of a moderate breath–alcohol content (BrAC of 0.40 mg/L) on binocular visual performance for different visual functions after inducing different levels of interocular differences with the use of filters. A total of 26 healthy young subjects were enrolled. The participants participated in two sessions: one without alcohol consumption and another after alcohol consumption. In each session and for the different filter conditions (subjects were wearing Bangerter foil of 0.8 and BPM2 fog filter on the dominant eye), monocular and binocular visual function was evaluated by measuring visual acuity, contrast sensitivity, visual discrimination capacity (and successively by calculating their corresponding binocular summations) and stereopsis (near and distance stereoacuity). In addition, interocular differences were calculated for different retinal–image quality and straylight parameters. All monocular and binocular visual functions were analyzed and stereopsis was significantly impaired by alcohol and filters (p < 0.05). Interocular differences for different ocular parameters and binocular summations for visual parameters were negatively affected by filters but not alcohol. Significant correlations (averaging all the experimental conditions analyzed) were found, highlighting: the higher the interocular differences, the lower the binocular summation and the poorer the stereopsis and, therefore, the worse the binocular visual performance.
Factors Associated with Quality of Life of Clinical Nurses: A Cross-Sectional Survey
Authors: Park Seul-Ki , Lee Kyoung-Sook
Published: January 2023

Nurses often have a heavy workload and struggle to maintain a good quality of life (QOL). The aim of this study was to explore the relationship between perceived stresses and sleep disturbance, and quality of life among Korean clinical nurses. A cross-sectional design was used to examine these relationships among 200 clinical nurses at three hospitals in South Korea. Standardized instruments were used, including the Perceived Stress Scale (PSS), Insomnia Severity Index (ISI), Eating Behavior Questionnaire (DEBQ), and the WHOQoL-BREF questionnaire. The data were analyzed using descriptive statistics, the t-test, ANOVA, the Scheffe test, Pearson’s correlation coefficient, and multiple regression analysis using SPSS/WIN 24.0 program. Multiple regression analysis showed that clinical nurses who had a subjective health status (β = 0.29, p = 0.001), perceived stress (β = −0.32, p < 0.001), and sleep disturbance (β = −0.21, p = 0.001) were more likely to have a higher quality of life. The explanatory power of the regression model was statistically significant at 36.7%. Multiple regression analysis showed that clinical nurses who had better subjective health status, lower perceived stress, and fewer sleep disturbances were more likely to have a higher quality of life.
The Structural Relationship between Basic Psychological Needs, Grit, and the Quality of Life of Individuals with Disabilities
Authors: Jang Deok-Jin , Oh Chae-Yun , Jun Mun-Gyu , Oh Kyung-Rok , Lee Joon-Hee , Jang Jusun , Park Sung-Un
Published: January 2023

Individuals with disabilities who engage in regular physical activity reduce their risk of diseases such as obesity and heart disease, as well as other risk factors; relieve tense emotions, and improve their quality of life via interaction with others. Despite these advantages, only one out of every four Koreans with a disability engages in physical activity. Grit is the ability to maintain interest and effort towards a goal in the face of adversity and failure. Grit can act as an important factor in increasing the psychological level of individuals with disabilities. We investigated the relationship between basic psychological needs, grit, and the quality of life of disabled individuals to determine if physical activities can improve their quality of life. Our dataset included 296 disabled individuals registered with the Korean Ministry of Health and Welfare. Using structural equation modelling, the direct and indirect effects of grit, quality of life, and psychological needs satisfaction such as competence, relatedness, and autonomy were examined. We found that competence positively affects consistency of interests (β = 0.150, t = 1.854), relatedness positively affects consistency of interests (β = 0.354, t = 4.409), and autonomy has no statistically significant effects (β = 0.101, t = 1.086). Second, competence positively affects perseverance of effort (β = 0.249, t = 3.206), autonomy negatively affects perseverance of effort (β = −0.269, t = −2.880), and relatedness has no statistically significant effects (β = −0.017, t = −0.249). Third, autonomy positively affects quality of life (β = 0.214, t = 2.349) while competence and relatedness had no statistically significant effects (β = −0.018, t = −0.208; β = 0.096, t = 1.288). Fourth, consistency of interests positively affects quality of life (β = 0.312, t = 4.191) while perseverance of effort had no statistically significant effects (β = −0.094, t = −1.480). Fifth, competence was found to have positive indirect effects on quality of life through grit. This study underscores the importance of addressing these three basic psychological needs and elements of grit when designing future quality of life interventions for disabled individuals.
The Influence of a 9-Week Movement Program on the Body Composition of 7- to 8-Year-Old Schoolchildren in the Eastern Cape of South Africa
Authors: Idamokoro Mere , Pienaar Anita E. , Gerber Barry , van Gent Maria M.
Published: January 2023

Pediatric obesity has become a growing global epidemic which has negative health consequences, including for South African children. This study aimed to determine the immediate and sustainable influences of a 9-week movement program on the body composition of 7 to 8-year-old school children in a rural area of South Africa. A two group, pre-test, post-test and re-test after six months experimental design was used to compare anthropometric measurements of the intervention group (IG) and control group (CG). Ninety-three schoolchildren (IG = 57; CG = 36) participated in the study. A 9-week movement program was followed twice a week for 30 min during school hours with an emphasis on improving BMI. Hierarchical Linear Modelling (HLM) was used to analyze the data with time, sex and group as predictors. Effect sizes was computed based on the Cohen’s d to assess the practical significance of findings. The intervention positively changed the waist circumference. The subscapular skinfold and BMI showed statistical and practically significant sustainable changes because of the intervention, although gender influenced these effects. School based movement interventions, focusing on improving fundamental movement skills (FMS), have the potential to contribute to a healthier BMI, skinfold thickness and circumferences among young children.
Small Changes in Patient Arrival and Consultation Times Have Large Effects on Patients’ Waiting Times: Simulation Analyses for Primary Care
Authors: Grot Matthias , Kugai Simon , Degen Lukas , Wiemer Isabel , Werners Brigitte , Weltermann Birgitta M.
Published: January 2023

(1) Background: Workflows are a daily challenge in general practices. The desired smooth work processes and patient flows are not easy to achieve. This study uses an operational research approach to illustrate the general effects of patient arrival and consultation times on waiting times. (2) Methods: Stochastic simulations were used to model complex daily workflows of general practice. Following classical queuing models, patient arrivals, queuing discipline, and physician consultation times are three key factors influencing work processes. (3) Results: In the first scenario, with patients arriving every 7.6 min and random consultation times, the individual patients’ maximum waiting time increased to more than 200 min. The second scenario with random patient arrivals and random consultation times increased the average waiting time by up to 30 min compared to patients arriving on schedule. A busy morning session based on the second scenario was investigated to compare two alternative intervention strategies to reduce subsequent waiting times. Both could reduce waiting times by a multiple for each minute of reduced consultation time. (4) Conclusions: Aiming to improve family physicians’ awareness of strategies for improving workflows, this simulation study illustrates the effects of strategies that address consultation times and patient arrivals.
The Impact of Social Media Shared Health Content on Protective Behavior against COVID-19
Authors: Mohammed Fathey , Al-Kumaim Nabil Hasan , Alzahrani Ahmed Ibrahim , Fazea Yousef
Published: January 2023

The use of social media has increased during the COVID-19 pandemic because people are isolated and working from home. The use of social media enhances information exchange in society and may influence public protective behavior against the COVID-19 pandemic. The purpose of this study is to identify the factors affecting public protective behavior when relying on COVID-19 pandemic-related content shared on social media. A model based on Protection Motivation Theory (PMT) was proposed and validated using a quantitative survey approach. A questionnaire was distributed to random respondents, and 488 responses were received and analyzed using Smart-PLS software. The findings showed that perceived risk, e-health literacy, public awareness, and health experts’ participation influence public protective behavior when using social media to share COVID-19-relevant content. The outcomes of this study can enhance government agencies’ and public health care authorities’ understanding of how to use social media to raise awareness and reduce panic among the public.
Prevalence and Mode of Birth in Late Fetal Mortality in Spain, 2016–2019
Authors: Hidalgo-Lopezosa Pedro , Cubero-Luna Ana María , García-Fernández Rubén , Jiménez-Ruz Andrea , Maestre-Luna María Isabel , Liébana-Presa Cristina , Rodríguez-Borrego María Aurora , López-Soto Pablo Jesús
Published: January 2023

(1) Background: The rate of cesarean sections in late fetal mortality remains high. We aimed to determine the prevalence of late fetal mortality in Spain and risk factors for cesarean birth in women with stillbirth ≥ 28 weeks gestation between 2016–2019. (2) Methods: A retrospective observational study with national data between 2016–2019. A total of 3504 births with fetal dead were included. Sociodemographic, obstetrical and neonatal variables were analyzed using univariate and multivariate logistic regression (MLR), with cesarean birth with a stillborn ≥ 28 weeks gestation as the dependent variable. (3) Results: The late fetal mortality rate was 2.8 × 1000; 22.7% of births were by cesarean section. Factors associated with cesarean were having a multiple birth (aOR 6.78); stillbirth weight (aOR 2.41); birth taking place in towns with over 50,000 inhabitants (aOR 1.34); and mother’s age ≥ 35 (aOR 1.23). (4) Conclusions: The late fetal mortality rate increased during the period. The performance of cesarean sections was associated with the mother’s age, obstetric factors and place of birth. Our findings encourage reflection on how to best put into practice national clinical and socio-educational prevention strategies, as well as the approved protocols on how childbirth should be correctly conducted.
Understanding the Influence of Patient Factors on Accuracy and Decision-Making in a Diagnostic Accuracy Study with Multiple Raters—A Case Study from Dentistry
Authors: Vach Kirstin , Schlueter Nadine , Ganss Carolina , Vach Werner
Published: January 2023

In diagnostic accuracy studies, the test of interest is typically applied only once in each patient. This paper illustrates some possibilities that arise when diagnoses are carried out by a sufficiently large number of multiple raters. In a dental study, sixty-one examiners were asked to diagnose 49 tooth areas with different grades of tissue loss (minor, moderate, and advanced) to decide whether dentine was exposed (positive status) or not (negative status). The true status was determined by histology (reference). For each tooth, the rate of correct decisions reflecting the difficulty to diagnose this tooth and the positive rate reflecting the perception of the tooth by the raters was computed. Meta-analytical techniques were used to assess the inter-tooth variation and the influence of tooth-specific factors on difficulty or perception, respectively. A huge variation in diagnostic difficulty and perception could be observed. Advanced tissue loss made diagnoses more difficult. The background colour and tissue loss were associated with perception and may hint to cues used by the raters. The use of multiple raters in a diagnostic accuracy study allows detailed investigations which make it possible to obtain further insights into the decision-making process of the raters.
Perceptions and Expectations of Academic Staff in Bucharest towards the COVID-19 Pandemic Impact on Dental Education
Authors: Iosif Laura , Țâncu Ana Maria Cristina , Didilescu Andreea Cristiana , Imre Marina , Pițuru Silviu Mirel , Ionescu Ecaterina , Jinga Viorel
Published: January 2023

Dental education was severely challenged by the COVID-19 pandemic worldwide. The evaluation of the viewpoint of the dental teachers of the Faculty of Dentistry at “Carol Davila” University of Medicine and Pharmacy in Bucharest, Romania, on these exceptional circumstances’ consequences was the objective of this paper. A cross-sectional study was conducted in April 2022, on the academic staff who reported their perceptions of the emotional and educational impact of the pandemic by completing a Google Forms questionnaire. Although a significant emotional impact of the pandemic was reported by over a third of the participants (31.2%), most of them being teachers of fifth-year dental students (p = 0.019), the perceived stress had an impact on the teaching performance in few of them (14%), the quality of sleep remaining unaffected in most of them (53.7%), whereas the level of anxiety was low (57%). An educational impact regarding the techno difficulties during the online transition was mentioned by few respondents (16.1%), with male teaching staff facing the fewest problems (p = 0.024), as well as low levels of difficulties in transmitting academic information (11.9), with men also being the most unaffected (p = 0.006). More than half of the participants (59.1%) rather see digital and/or virtual education during the pandemic as having adverse effects on the educational system, the most sceptical being teachers of the fifth (p = 0.001) and sixth years (p = 0.001). The COVID-19 pandemic affected the academic staff of the Faculty of Dentistry at “Carol Davila” University of Medicine and Pharmacy in Bucharest, Romania, not only at a personal level but also at a professional, pedagogical one, due to the introduction of the online teaching system followed by the hybrid one. Age group, gender, and teaching year differentiated the degree of emotional and educational impairment.
A Meta-Analysis of the Influence on Inflammatory Factors in Type 2 Diabetes among Middle-Aged and Elderly Patients by Various Exercise Modalities
Authors: Yang Weijun , Jiao Haotian , Xue Yizhang , Wang Lishuo , Zhang Ye , Wang Boqian , Teng Ziyi , Li Junyan , Zhao Haotian , Liu Chang
Published: January 2023

The purpose of this study was to investigate the effects of various exercise modalities on inflammatory factors in middle-aged and elderly patients with type 2 diabetes (MEPT2D), as lifestyle changes, such as physical activity and dietary modifications, are considered important in the prevention of type 2 diabetes. For the study methodology, Pubmed, CNKI, EBSCO, Wanfang Data, and Web of Science were selected for the search. The methodological quality of the included studies was assessed by the Cochrane Risk of Bias (ROB) tool, and statistically analyzed using the RevMan 5.4.1 analysis software, which included 18 investigations involving 853 study subjects. Meta-analysis findings indicated that aerobic training (AT), resistance training (RT), combined training (CT), and high-intensity interval training (HIIT) showed significant reductions in CRP, TNF-α, IL-6, and IL-10 levels in MEPT2D. Among them, HIIT was superior to other training modalities in reducing TNF-α levels, while CT was superior to AT, RT, and HIIT in decreasing IL-6, IL-10, and CRP in MEPT2D. Meanwhile, RT had limited effects in reducing CRP and TNF-α levels in MEPT2D. However, HIIT had no significant effect on IL-6 and IL-10 in MEPT2D. In conclusion, long-term regular AT, RT, CT, and HIIT all contributed to the reduction of inflammatory status (CRP, TNF-α, IL-6, and IL-10) in MEPT2D, while CT (for CRP, IL-6, and IL-10) and HIIT (for TNF-α) represent the best approaches to counteract the inflammatory response in MEPT2D.
Physical Activity Level, Barriers, and Facilitators for Exercise Engagement for Chronic Community-Dwelling Stroke Survivors in Low-Income Settings: A Cross-Sectional Study in Benin
Authors: Noukpo Sènadé Inès , Triccas Lisa Tedesco , Bonnechère Bruno , Adoukonou Thierry , Feys Peter , Kossi Oyéné
Published: January 2023

After a stroke incident, physical inactivity is common. People with stroke may perceive several barriers to performing physical activity (PA). This study aimed to document the PA level and understand the barriers and facilitators to engaging in PA for community-dwelling stroke survivors in Benin, a lower middle-income country. A cross-sectional study was conducted in three hospitals in Benin. Levels of PA were recorded by means of the Benin version of the International Physical Activity Questionnaire long form (IPAQ-LF-Benin), which is validated for stroke survivors in Benin. The perceived exercise facilitators and barriers were assessed by the Stroke Exercise Preference Inventory-13 (SEPI-13). A descriptive analysis and associations were performed with a Confidence Interval of 95% and <0.05 level of significance. A total of 87 participants (52 men, mean age of 53 ± 10 years, mean time after a stroke of 11 (IQR: 15) months and an average of 264.5 ± 178.9 m as distance on the 6 min walking test (6MWT) were included. Overall, stroke survivors in Benin reached a total PA of 985.5 (IQR: 2520) metabolic equivalent (METs)-minutes per week and were least active at work, domestic, and leisure domains with 0 MET-minutes per week. The overview of PA level showed that 52.9% of participants performed low PA intensity. However, 41.4% performed moderate PA or walking per day for at least five days per week. Important perceived barriers were lack of information (45.3%), hard-to-start exercise (39.5%), and travelling to places to exercise (29.9%). The preference for exercise was with family or friends, outdoors, for relaxation or enjoyment (90.2%), and receiving feedback (78.3%). Several socio-demographic, clinical, and community factors were significantly associated with moderate or intense PA (p < 0.05) in stroke survivors in this study. Our findings show that the PA level among chronic stroke survivors in Benin is overall too low relative to their walking capacity. Cultural factors in terms of the overprotection of the patients by their entourage and/or the low health literacy of populations to understand the effect of PA on their health may play a role. There is a need for new approaches that consider the individual barriers and facilitators to exercise.
Prevalence of Anemia among Children and Adolescents of Bangladesh: A Systematic Review and Meta-Analysis
Authors: Kundu Shoumik , Alam Sayeda Sadia , Mia Md Al-Tareq , Hossan Tareq , Hider Phil , Khalil Md. Ibrahim , Musa Kamarul Imran , Islam Md Asiful
Published: January 2023

The prevalence of anemia is high among children and adolescents in low- and middle-income countries because of undernutrition resulting from their poor socioeconomic status and lack of knowledge on proper nutrition. We conducted a systematic review and meta-analysis to determine the prevalence of anemia among children and adolescents aged between 6 months and 19 years in Bangladesh. Databases such as PubMed, Scopus, and Google Scholar were searched to identify the studies that reported the prevalence of anemia among children and adolescents. A total of 24 studies, including the data of 14,062 cases, were included in the systematic review and meta-analysis of the time period between 1997 and 2019. The random-effects model was used to calculate the summary estimates. The protocol was registered with PROSPERO (CRD42021246960). The pooled prevalence of anemia, iron deficiency anemia (IDA), and non-severe and severe anemia were 46.8% [95% CI: 36.0–57.6], 13.6% [95% CI: 8.0–19.2], 56.4% [95% CI: 39.6–73.1] and 0.7% [95% CI: 0.1–1.4], respectively. Prevalence of anemia exhibited the highest among the children aged ≤2 years. Briefly, 91.67% of the studies were of high quality. No significant publication bias was found; however, two outlier studies were detected. The prevalence of anemia among children and adolescents was estimated as high in Bangladesh.
A Survey of the Landscape Visibility Analysis Tools and Technical Improvements
Authors: Wu Zhiqiang , Wang Yuankai , Gan Wei , Zou Yixuan , Dong Wen , Zhou Shiqi , Wang Mo
Published: January 2023

Visual perception of the urban landscape in a city is complex and dynamic, and it is largely influenced by human vision and the dynamic spatial layout of the attractions. In return, landscape visibility not only affects how people interact with the environment but also promotes regional values and urban resilience. The development of visibility has evolved, and the digital landscape visibility analysis method allows urban researchers to redefine visible space and better quantify human perceptions and observations of the landscape space. In this paper, we first reviewed and compared the theoretical results and measurement tools for spatial visual perception and compared the value of the analytical methods and tools for landscape visualization in multiple dimensions on the principal of urban planning (e.g., complex environment, computational scalability, and interactive intervention between computation and built environment). We found that most of the research was examined in a static environment using simple viewpoints, which can hardly explain the actual complexity and dynamic superposition of the landscape perceptual effect in an urban environment. Thus, those methods cannot effectively solve actual urban planning issues. Aiming at this demand, we proposed a workflow optimization and developed a responsive cross-scale and multilandscape object 3D visibility analysis method, forming our analysis model for testing on the study case. By combining the multilandscape batch scanning method with a refined voxel model, it can be adapted for large-scale complex dynamic urban visual problems. As a result, we obtained accurate spatial visibility calculations that can be conducted across scales from the macro to micro, with large external mountain landscapes and small internal open spaces. Our verified approach not only has a good performance in the analysis of complex visibility problems (e.g., we defined the two most influential spatial variables to maintain good street-based landscape visibility) but also the high efficiency of spatial interventions (e.g., where the four recommended interventions were the most valuable), realizing the improvement of intelligent landscape evaluations and interventions for urban spatial quality and resilience.
Effect of the Case for Carpule as a Visual Passive Distraction Tool on Dental Fear and Anxiety: A Pilot Study
Authors: Barbosa Nayara Bertoldo , Rodrigues Bárbara Rocha , Madalena Isabela Ribeiro , de Menezes Fernando Carlos Hueb , Lepri César Penazzo , de Oliveira Maria Beatriz Carvalho Ribeiro , Campos Michelle Gomides Dumont , Oliveira Maria Angélica Hueb de Menezes
Published: January 2023

The present cross-over study aimed to evaluate the effect of a visual passive distraction tool, a case for carpule, in the management of fear and anxiety during invasive dental treatment. Children between the ages of 4 and 8 years that need at least two treatments with inferior alveolar nerve block were selected. All the included children received two treatments at different moments: in one treatment, local anesthesia was performed with a visual passive distraction tool, the crocodile case, for carpule (experimental); in the other treatment, local anesthesia was performed without the device (control). An evaluation of the anxiety levels was performed by measuring the heart rate and salivary cortisol levels. Wilcoxon’s nonparametric test was used for a data analysis (p < 0.05). The mean heart rate with the visual passive distraction device fell from 81.8 to 78.1, while the control rose from 91.4 to 100 (p < 0.05). The mean salivary cortisol levels with the visual passive distraction device fell from 2.0 to 1.6, while in the control, they rose from 2.1 to 2.4 (p < 0.05). In conclusion, the crocodile case for carpule as a visual passive distraction device contributed positively to the management of fear and anxiety during inferior alveolar nerve block in children.
Differences in Clinical Tests for Assessing Lateral Epicondylitis Elbow in Adults Concerning Their Physical Activity Level: Test Reliability, Accuracy of Ultrasound Imaging, and Relationship with Energy Expenditure
Authors: Soares Marcos Martins , Souza Patrícia Colombo , Ribeiro Ana Paula
Published: January 2023

Background: Physical activity (PA) can generate physical stress on the musculoskeletal system. Thus, the aim of the current study was to assess the influence of the level of physical activity on clinical tests used in the diagnosis of lateral epicondylitis in adults, intertest reliability and accuracy based on ultrasound imaging, and relationship with energy expenditure. Methods: 102 adults with lateral epicondylitis were assessed via an International Physical Activity Questionnaire and divided according to PA level: low (n = 19) moderate (n = 42) or high (n = 41). Pain (visual analog scale), Cozen’s and Mill’s clinical tests and ultrasound exams were performed. Results: The Cozen’s and Mill’s tests differed among PA levels. Excellent reliability was found for Mill’s test for PA levels and the ultrasound exam (low ICC = 1.0, moderate ICC = 0.82 and high ICC = 0.99). Good reliability was found for Cozen’s test (low ICC = 0.80, moderate ICC = 0.74 and high ICC = 0.73), but with significant differences between the ultrasound exams. The Cozen’s and Mill’s clinical tests had no relationship to the level of energy expenditure for PA levels. Conclusion: Mill’s test was reliable and accurate for the PA levels. Intertest reliability was poor for the PA levels. Mill’s test proved to be accurate based on the ultrasound exam. The pain caused by the tests was not related to the level of energy expenditure.
Suicidal Ideation in Iraqi Medical Students Based on Research Using PHQ-9 and SSI-C
Authors: Al-Imam Ahmed , Motyka Marek A. , Hoffmann Beata , Basil Safwa , Al-Hemiary Nesif
Published: January 2023

Suicidal ideation is a spectrum of contemplations, wishes, and preoccupations with suicide. Its prevalence is ambiguous in Iraq, especially among the youth. We aim to survey the prevalence of suicidal ideation among Iraqi students and explore its risk factors. We surveyed Iraqi undergraduate medical students (n = 496) using two psychometric tools, the PHQ-9 and Beck’s SSI-C. We also explored potential risk factors, including the students’ attributes, socio-demographics, and history of mental illnesses. The current study included males (23.8%) and females (76.2%) in their early twenties (21.73 ± 0.11). Concerning PHQ-9 and SSI-C, most students had either moderate (28%) or mild depression (27.8%), while those with suicidal ideation accounted for an alarming 64.9%. The strongest association existed between the SSI-C and PHQ-9 scores (p = 0.001, OR = 4.70). Other associations existed with the personal history of mental illness (p < 0.001, OR = 2.87) and the family history of suicidality (p = 0.006, OR = 2.28). Path analysis highlighted four suicidal ideation predictors, including the PHQ-9 score (standardized estimate = 0.41, p < 0.001), personal history of mental illness (0.16, p < 0.001), previous psychiatric consultation (0.12, p = 0.002), and family history of suicidality (0.11, p = 0.005). Suicidal ideation is highly prevalent among Iraqi students. Univariable testing, multivariable analyses, and structural modeling yielded congruent results. The students’ inherent rather than inherited attributes influenced the phenomenon the most, which is in harmony with Durkheim’s theory on the social roots of suicide. We encourage psychiatrists and psychology counselors to be vigilant concerning these risk factors among potential suicidal ideation victims.
Seasonal and Spatial Variations of the Oxidative Properties of Ambient PM2.5 in the Po Valley, Italy, before and during COVID-19 Lockdown Restrictions
Authors: Pietrogrande Maria Chiara , Colombi Cristina , Cuccia Eleonora , Dal Santo Umberto , Romanato Luisa
Published: January 2023

This study describes the chemical and toxicological characteristics of fine particulate matter (PM2.5) in the Po Valley, one of the largest and most polluted areas in Europe. The investigated samples were collected in the metropolitan area of Milan during the epidemic lockdown and their toxicity was evaluated by the oxidative potential (OP), measured using ascorbic acid (OPAA) and dithiothreitol (OPDTT) acellular assays. The study was also extended to PM2.5 samples collected at different sites in the Po Valley in 2019, to represent the baseline conditions in the area. Univariate correlations were applied to the whole dataset to link the OP responses with the concentrations of the major chemical markers of vehicular and biomass burning emissions. Of the two assays, OPAA was found mainly sensitive towards transition metals released from vehicular traffic, while OPDTT towards the PM carbonaceous components. The impact of the controlling lockdown restrictions on PM2.5 oxidative properties was estimated by comparing the OP values in corresponding time spans in 2020 and 2019. We found that during the full lockdown the OPAA values decreased to 80–86% with respect to the OP data in other urban sites in the area, while the OPDTT values remained nearly constant.
2021/22 Rugby Europe Injury Surveillance Report: SuperCup, Under-20, and Under-18 Championship
Authors: Cruz-Ferreira António Miguel , Montocchio Agathe , Usova-Akula Elena , Tuccelli Philippe , Marty Florent
Published: January 2023

We conducted a prospective cohort study to determine the incidence rate and characterization of the injuries sustained by players during the 2021/22 season of the Rugby Europe SuperCup, Under-20, and Under-18 championships. Team medics reported the injuries, using an online platform. Ethical approval and informed consent were obtained. The overall incidence of injuries ranged from 33.33 (95% CI: 18.97–54.60) in the under-18s to 83.33 (95% CI: 60.34–112.40) in the under-20s, while in the SuperCup it was 41.35 (95% CI: 30.30–55.18) injuries per 1000 player-match-hours. Injury severity (mean days) was higher in the SuperCup (38.33) and lower in the Under-18 tournament (28.50). Lower limb and soft tissue were the most common type of injuries. Tackles caused almost two-thirds of all injuries. Concussion accounted for 10.0% to 25.6% of all injuries. Our data are consistent with previous reports for similar levels of competition and age grades. The injury incidence was higher in the senior competitions (Under-20s and SuperCup). However, for the under-20s, injury rates were higher than in the SuperCup. This might be related to the competition format; however, more studies need to be conducted in the future. Concussion is a common injury and the protocols used at this level seems to be effective to identify it.
Optimization of the Territorial Spatial Patterns Based on MOP and PLUS Models: A Case Study from Hefei City, China
Authors: Yu Ran , Cheng Hongsheng , Ye Yun , Wang Qin , Fan Shuping , Li Tan , Wang Cheng , Su Yue , Zhang Xingyu
Published: January 2023

Optimization of the territorial spatial patterns can promote the functional balance and utilization efficiency of space, which is influenced by economic, social, ecological, and environmental factors. Consequently, the final implementation of spatial planning should address the issue of sustainable optimization of territorial spatial patterns, driven by multiple objectives. It has two components—the territorial spatial scale prediction and its layout simulation. Because a one-sided study of scale or layout is divisive, it is necessary to combine the two to form complete territorial spatial patterns. This paper took Hefei city as an example and optimized its territorial spatial scale using the multiple objective programming (MOP) model, with four objective functions. A computer simulation of the territorial spatial layout was created, using the patch-generating land use simulation (PLUS) model, with spatial driving factors, conversion rules, and the scale optimization result. To do this, statistical, empirical, land utilization, and spatially driven data were used. The function results showed that carbon accumulation and economic and ecological benefits would be ever-increasing, and carbon emissions would reach their peak in 2030. The year 2030 was a vital node for the two most important land use types in the spatial scale—construction land and farmland. It was projected that construction land would commence its transition from reduced to negative growth after that time, and farmland would start to rebound. The simulation results indicated that construction land in the main urban area would expand primarily to the west, with supplemental expansion to the east and north. In contrast, construction land in the counties would experience a nominal increase, and a future ecological corridor would develop along the route south of Chaohu County–Chaohu Waters–Lujiang County–south of Feixi County.
Contamination Landscapes: Spatio-Temporal Record and Analysis of Pathogens in Clinical Settings
Authors: Höser Christoph , Kistemann Thomas
Published: January 2023

Nosocomial outbreaks require quick epidemiological clarification of possible chains of infection, since the pathogen usually has a head start that has to be caught up. Identification of people and areas at risk is crucial for efficient confinement. This paper describes a concept which can be applied to healthcare settings. The application skips the time-consuming and imperfect reconstruction of direct and indirect contacts. Indoor mobility of people and devices are instead measured precisely, and the mobility history is used to construct a spatio-temporal ‘landscape of infection’. This landscape allows for the calculation of a modelled ‘contamination landscape’ (CL) adding location-based prolongation of infectivity. In that way, the risk per person can be derived in case of an outbreak. The CL concept is extremely flexible and can be adapted to various pathogen-specific settings. The combination of advanced measurements and specific modelling results in an instant list of possible recipients who need to be examined directly. The modelled, pathogen-specific parameters can be adjusted to get as close as possible to the results of mass screenings.
Barriers and Enablers for the Use of Digital Interactive Television in Nursing Home Settings: An Interview Case Study with Older Adults and Professionals
Authors: Naudé Bérangère , Rigaud Anne-Sophie , Kamali Laila , Pino Maribel
Published: January 2023

Increasingly, public health programs are recommending the use of information and communication technologies to address the psychosocial needs of Older Adults (OAs). Recently, several applications that allow access to communication and stimulation functionalities using digital interactive television (DiTV) have been developed. The use of the television interface to access digital services seems to help meet several accessibility and usability needs of OAs. However, its use entails other challenges related to different dimensions (organizational, technological, ethical, etc.). This study aims to identify the factors that enable or hinder the use of DiTV by OAs living in geriatric institutions. A qualitative interview case study was conducted in three French geriatric facilities. A total of 25 semi-structured interviews were carried out with residents and care professionals, between February and April 2022, to identify enablers and barriers to DiTV use. Data were processed using a thematic deductive analysis inspired by a multidimensional Health Technology Assessment model. The analysis showed that DiTV use may be limited by organizational (e.g., workload), technological (e.g., ergonomic issues), human (e.g., health issues), ethical (e.g., privacy), and safety factors (e.g., frustration due to technical problems). A summary of these factors and five recommendations for DiTV implementation in geriatric settings are presented in this paper.