bluuebunny/crossref_metadata_2025 huggingface dataset:
Vertical transfer and functional characterization of cotton seed core microbiome
Authors: Wu Chongdie , Zhang Xin , Fan Yongbin , Ye Jingyi , Dong Lingjun , Wang YuXiang , Ren YinZheng , Yong HongHong , Liu Ruina , Wang Aiying
Published: January 2024

Introduction

Microbiome within plant tissues is pivotal for co-evolution with host plants. This microbiome can colonize the plant, with potential transmission via seeds between parents and offspring, affecting seedling growth and host plant adaptability to the environment.

Methods

We employed 16S rRNA gene amplicon analysis to investigate the vertical distribution of core microbiome in cotton seeds across ecological niches [rhizosphere, root, stem, leaf, seed and seed-P (parental seed)] of the three cotton genotypes.

Results

The findings demonstrated a significant decrease in microbiome diversity and network complexity from roots, stems, and leaves to seeds. The microenvironment exerted a more substantial influence on the microbiome structure of cotton than the genotypes. The core endophytic microorganisms in cotton seeds comprised 29 amplicon sequence variants (ASVs) affiliated with Acidimicrobiia, Alphaproteobacteria, Bacilli, Bacteroidia, Clostridia, Gammaproteobacteria, and unclassified_Proteobacteria. These vertically transmitted taxa are widely distributed in cotton plants. Through 16S rRNA gene-based function prediction analysis of the cotton microbiome, we preliminarily understood that there are potential differences in metabolic capabilities and phenotypic traits among microbiomes in different microhabitats.

Discussion

In conclusion, this study demonstrated the crucial role of the microenvironment in influencing the cotton microbiome and offered insights into the structures and functions of the cotton seed microbiome, facilitating future crop yield enhancement through core seed microbiome regulation.
Residual effects of combined vibratory and plantar stimulation while seated influences plantar pressure and spatiotemporal gait measures in individuals with Parkinson’s disease exhibiting freezing of gait
Authors: Phuenpathom Warongporn , Panyakaew Pattamon , Vateekul Peerapon , Surangsrirat Decho , Bhidayasiri Roongroj
Published: January 2024

Introduction

Combined plantar pressure and vibratory stimulation has been shown to decrease freezing of gait (FOG) episodes and improve spatiotemporal gait parameters compared to single stimulation in Parkinson’s disease (PD) patients with FOG. However, the effect of combined plantar stimulations on plantar pressure analysis has never been explored.

Methods

Forty PD patients with frequent FOG were allocated to either FOG shoes embedded with a 100 Hz vibratory stimulation at the Achilles tendons and a soft thickened silicone pad at the hallux and sole, or sham shoes with a non-working vibratory motor and a flat non-pressure silicone pad (20 patients per arm) while seated for 96 s. The objective gait and plantar pressure analysis were measured immediately after the stimulation. Outcomes included the normalized percentage of changes in percent FOG (%FOG) and plantar pressure in the heel-strike and push-off phase that were compared between pre- and post-stimulations.

Results

The FOG shoes group showed significantly decreased %FOG (81.5 ± 28.9% vs. 6.8 ± 22.1%, p < 0.001), plantar pressure in the heel-strike (47.8 ± 43.7% vs. 4.3 ± 9.8%, p < 0.001), plantar pressure in the push-off (57.7 ± 59.6% vs. 6.2 ± 11.6%, p < 0.001), force time integral (FTI) (40.9 ± 32.5% vs. 6.6 ± 17.3%, p < 0.001), and decreased heel contact time (19.3 ± 12.3% vs. 22.7 ± 32.5%, p < 0.001) when compared to the sham group. There was a strong negative correlation between %FOG and peak plantar pressure (r = −0.440, p = 0.005), plantar pressure in the heel-strike (r = −0.847, p < 0.001).

Conclusion

Our study demonstrated that the FOG shoe could decrease FOG episodes by improving the heel-strike pressure, toe push-off and normalized heel-to-toe plantar pressure, suggesting that modification inputs from the peripheral sensory systems might significant improvement in FOG in PD.
Domingo Sánchez y Sánchez (1860–1947): Cajal’s man on the nervous system of invertebrates
Authors: Serrano-Herrera Adela , Espinosa-Sanchez Juan Manuel
Published: January 2024

Domingo Sánchez y Sánchez (1860–1947), a distinguished disciple of Santiago Ramón y Cajal, played a fundamental role in the Spanish School of Neurohistology through the meticulous use of diverse staining and microscopic techniques in the study of the histology and physiology of the invertebrate nervous system, generating valuable contributions that were recognized and cited by the scientific community. His research covered a wide range of areas: he was initially an anthropologist and zoologist, later earning a doctorate in Medicine and specializing in the neurohistology of invertebrates, including the detailed study of the retina and nerve centers of insects, and the discovery of histolysis in nerve centers of insect larvae during metamorphosis, challenging scientific paradigms of the time. Furthermore, Sánchez’s work on the neurofibrils of insects was crucial in supporting Cajal’s neuronal theory and refuting Bethe and Apathy’s reticularist hypothesis. Additionally, he also made preliminary observations of the Golgi apparatus, the lysosomal system, the endoplasmic reticulum, and the sarcoplasmic reticulum of skeletal muscles (Cajal-Fusari network). Domingo Sánchez y Sánchez’s exceptional scientific research and contributions to neurohistology in 20th century Spain continue to serve as a significant legacy.

Life science identifiers

Apis mellifera: urn:lsid:zoobank.org:act:9082C709-6347-4768-A0DC-27DC44400CB2

Helix aspersa: urn:lsid:zoobank.org:act:9099927E-24DF-4F89-B352-6B7902CD4A38
Gradients of O-information highlight synergy and redundancy in physiological applications
Authors: Scagliarini Tomas , Sparacino Laura , Faes Luca , Marinazzo Daniele , Stramaglia Sebastiano
Published: January 2024

The study of high order dependencies in complex systems has recently led to the introduction of statistical synergy, a novel quantity corresponding to a form of emergence in which patterns at large scales are not traceable from lower scales. As a consequence, several works in the last years dealt with the synergy and its counterpart, the redundancy. In particular, the O-information is a signed metric that measures the balance between redundant and synergistic statistical dependencies. In spite of its growing use, this metric does not provide insight about the role played by low-order scales in the formation of high order effects. To fill this gap, the framework for the computation of the O-information has been recently expanded introducing the so-called gradients of this metric, which measure the irreducible contribution of a variable (or a group of variables) to the high order informational circuits of a system. Here, we review the theory behind the O-information and its gradients and present the potential of these concepts in the field of network physiology, showing two new applications relevant to brain functional connectivity probed via functional resonance imaging and physiological interactions among the variability of heart rate, arterial pressure, respiration and cerebral blood flow.
The genetic risk of acute lymphoblastic leukemia and its implications for children of Latin American origin
Authors: de Smith Adam J. , Jiménez-Morales Silvia , Mejía-Aranguré Juan Manuel
Published: January 2024

Acute lymphoblastic leukemia (ALL) is the most common cancer in children, and disproportionately affects children of Hispanic/Latino ethnicity in the United States, who have the highest incidence of disease compared with other racial/ethnic groups. Incidence of childhood ALL is similarly high in several Latin American countries, notably in Mexico, and of concern is the rising incidence of childhood ALL in some Hispanic/Latino populations that may further widen this disparity. Prior studies have implicated common germline genetic variants in the increased risk of ALL among Hispanic/Latino children. In this review, we describe the known disparities in ALL incidence as well as patient outcomes that disproportionately affect Hispanic/Latino children across the Americas, and we focus on the role of genetic variation as well as Indigenous American ancestry in the etiology of these disparities. Finally, we discuss future avenues of research to further our understanding of the causes of the disparities in ALL incidence and outcomes in children of Latin American origin, which will be required for future precision prevention efforts.
The biological functions and related signaling pathways of SPON2
Authors: Zhang Jingrun , Liu Ge , Liu Yuchen , Yang Pei , Xie Junyuan , Wei Xiaowei
Published: January 2024

Spondin-2 (SPON2), also referred to as M-spondin or DIL-1, is a member of the extracellular matrix protein family known as Mindin-F-spondin (FS). SPON2 can be used as a broad-spectrum tumor marker for more than a dozen tumors, mainly prostate cancer. Meanwhile, SPON2 is also a potential biomarker for the diagnosis of certain non-tumor diseases. Additionally, SPON2 plays a pivotal role in regulating tumor metastasis and progression. In normal tissues, SPON2 has a variety of biological functions represented by promoting growth and development and cell proliferation. This paper presents a comprehensive overview of the regulatory mechanisms, diagnostic potential as a broad-spectrum biomarker, diverse biological functions, involvement in various signaling pathways, and clinical applications of SPON2.
Complete mitochondrial genome of Syzygium samarangense reveals genomic recombination, gene transfer, and RNA editing events
Authors: Lu Guilong , Li Qing
Published: January 2024

Wax apple (Syzygium samarangense) is a commercial fruit that belongs to one of the most species-rich tree genera in the world. We report here the first complete S. samarangense mitogenome obtained using a hybrid assembly strategy. The mitogenome was a 530,242 bp circular molecule encoding 61 unique genes accounting for 7.99% of the full-length genome. Additionally, 167 simple sequence repeats, 19 tandem repeats, and 529 pairs of interspersed repeats were identified. Long read mapping and Sanger sequencing revealed the involvement of two forward repeats (35,843 bp and 22,925 bp) in mediating recombination. Thirteen homologous fragments in the chloroplast genome were identified, accounting for 1.53% of the mitogenome, and the longest fragment was 2,432 bp. An evolutionary analysis showed that S. samarangense underwent multiple genomic reorganization events and lost at least four protein-coding genes (PCGs) (rps2, rps7, rps11, and rps19). A total of 591 RNA editing sites were predicted in 37 PCGs, of which nad1-2, nad4L-2, and rps10-2 led to the gain of new start codons, while atp6-1156, ccmFC-1315 and rps10-331 created new stop codons. This study reveals the genetic features of the S. samarangense mitogenome and provides a scientific basis for further studies of traits with an epistatic basis and for germplasm identification.
Minor effect of wind exposure and littoral slope on macrophyte characteristics in non-impacted lowland lakes of Poland
Authors: Kolada Agnieszka , Kutyła Sebastian , Bielczyńska Aleksandra
Published: January 2024

Aquatic vegetation is a reliable indicator of the ecological condition of surface waters. Abundance, composition and spatial structure of aquatic communities are shaped by an array of factors, which include both natural abiotic features of an ecosystem and external influences. We investigated whether the physical features, i.e., wind exposure and slope of the lake basin, have a significant impact on the taxonomic composition and spatial structure of macrophyte communities from non-impacted, highly alkaline, lowland lakes of the European plains (Poland). We further examined whether these features can affect the classification of the ecological status of lakes assessed in accordance with the Water Framework Directive requirements. Morphological, botanical and physicochemical data from 260 transects in 16 non-disturbed lakes of Polish lowlands surveyed in the years 2011–2016 were analysed. For each transect, littoral slope and wind exposure were calculated. Additionally, the total phosphorus concentration was used as a proxy of water trophy. The relationships between environmental variables and macrophyte indices as well as the syntaxonomic composition of aquatic and rush vegetation (dependent variables) were analysed using multidimensional ordination techniques (redundancy analysis, variation partitioning and indicator values), correlation and regression analysis. Among the three analysed environmental factors (littoral slope, wind exposure and water trophy), in almost all cases the latter explained the highest variance in the macrophyte community, while the contribution of the first two was at most moderate, weak or usually statistically insignificant. However, lakes with steeper slopes were more frequently inhabited by stoneworts and had better ecological status than those with a gentle littoral shape. This may be attributed to the links between lake morphometry and rate of eutrophication, with deep lakes supporting more effective dilution of substances. Furthermore, lower light requirements of charophytes than of higher plants and the capacity to growth in unstable sediments facilitate charophyte establishment in deeper and steeper parts of the littoral over higher plants. Our findings suggest that in lowland lakes with relatively small areas, moderate depths and low wind exposure typical of European plains, slopes and weaving do not hamper vegetation development and do not negatively affect the macrophyte assessment of ecological status. In such ecosystems, eutrophication seems to be a more important factor determining aquatic vegetation than physical features.
Plant biostimulants as natural alternatives to synthetic auxins in strawberry production: physiological and metabolic insights
Authors: Cardarelli Mariateresa , El Chami Antonio , Rouphael Youssef , Ciriello Michele , Bonini Paolo , Erice Gorka , Cirino Veronica , Basile Boris , Corrado Giandomenico , Choi Seunghyun , Kim Hye-Ji , Colla Giuseppe
Published: January 2024

The demand for high-quality strawberries continues to grow, emphasizing the need for innovative agricultural practices to enhance both yield and fruit quality. In this context, the utilization of natural products, such as biostimulants, has emerged as a promising avenue for improving strawberry production while aligning with sustainable and eco-friendly agricultural approaches. This study explores the influence of a bacterial filtrate (BF), a vegetal-derived protein hydrolysate (PH), and a standard synthetic auxin (SA) on strawberry, investigating their effects on yield, fruit quality, mineral composition and metabolomics of leaves and fruits. Agronomic trial revealed that SA and BF significantly enhanced early fruit yield due to their positive influence on flowering and fruit set, while PH treatment favored a gradual and prolonged fruit set, associated with an increased shoot biomass and sustained production. Fruit quality analysis showed that PH-treated fruits exhibited an increase of firmness and soluble solids content, whereas SA-treated fruits displayed lower firmness and soluble solids content. The ionomic analysis of leaves and fruits indicated that all treatments provided sufficient nutrients, with heavy metals within regulatory limits. Metabolomics indicated that PH stimulated primary metabolites, while SA and BF directly affected flavonoid and anthocyanin biosynthesis, and PH increased fruit quality through enhanced production of beneficial metabolites. This research offers valuable insights for optimizing strawberry production and fruit quality by harnessing the potential of natural biostimulants as viable alternative to synthetic compounds.
Vegan spread applications of alternative protein from torula yeast: product development and consumer perception
Authors: Gärtner Ann-Kristin , Matullat Imke , Genuttis Darleen , Engelhardt Sarah , Sveinsdóttir Kolbrún , Niimi Jun , Rusu Alexandru
Published: January 2024

Alternative protein sources are gaining attraction in food industry and consumers. Proteins obtained by single-cell organisms, such as torula yeast, are of enormous interest, as they are highly scalable, efficient, and sustainable, and the production costs are comparably low. Nevertheless, proteins obtained from yeasts are still mostly known and studied for feed applications, despite their nutritional, functional, and sensory benefits for various food applications. Testing consumer acceptance of products, especially products containing alternative proteins provides insights into, e.g., market success, consumer perception, and optimization potential. In this study, the development of two vegan spread powders, high in protein and containing torula yeast as an alternative protein source, is introduced. The result of food product development using torula yeast were “Leberwurst”-style (14.7% protein) and a “Balkan”-style (9.7% protein) spreads both meeting the criteria “at least 20% kcal from proteins of total product kcal” and thus claimable as “high-protein.” The application of the alternative protein from torula yeast within the final products was studied by a consumer acceptance test (n = 123) within three different countries (Germany, Iceland, and Sweden). Consumers also rated their trust in food production actors, the food industry in particular, and their willingness to try new foods. Overall, both spreads received acceptance values in the range of “like slightly.” It is noticeable that the consumers liked the spread “Balkan style” more than “Leberwurst”-style. The background variables revealed higher neophobic characteristics of Icelandic consumers compared with Swedish or German consumers. However, German consumers felt transparency, and communication was missing, but Icelandic consumers, in general, had more trust in the overall food value chain. This knowledge allows for the development of strategies that address cultural-specific barriers and capitalize on cultural values that promote openness to culinary innovation. The identification of cultural variations in consumer preferences emphasizes the need for customized approaches to product development and marketing. These findings could have implications for businesses and policymakers in understanding and catering to the preferences and concerns of consumers in these respective countries. Businesses might benefit from emphasizing transparency and improving communication strategies. This could involve providing clear information about the sourcing, production, and other aspects of the food value chain.
Argon cold atmospheric plasma eradicates pathogens in vitro that are commonly associated with canine bacterial keratitis
Authors: Marx Anne Helene , Oltmanns Hilke , Meißner Jessica , Verspohl Jutta , Fuchsluger Thomas , Busse Claudia
Published: January 2024

Purpose

To investigate the antimicrobial effect of cold atmospheric plasma (CAP) on pathogens associated with canine bacterial keratitis.

Materials and methods

Pseudomonas aeruginosa, Staphylococcus pseudintermedius, and Streptococcus canis strains, which were obtained from dogs with infectious keratitis, were subjected to testing. For each species, four isolates and a reference strain were cultivated on Columbia sheep blood agar and treated with the kiNPen Vet® plasma pen from Neoplas GmbH, Greifswald, Germany. Various continuous treatment durations (0.5, 2, and 5 min) were applied, along with a 0.5-min treatment repeated four times at short intervals. These treatments were conducted at distances of 3 and 18 mm between the agar surface and the pen.

Results

CAP treatment reduced bacterial growth in all three species. The most effective treatment duration was 5 min at 3 mm distance, resulting in inhibition zones ranging from 19 to 22 mm for P. aeruginosa, 26–45 mm for S. pseudintermedius and an overall reduction of bacterial growth for Str. canis. Inhibition zones were smaller with decreasing treatment duration and larger distance. Treatment times of 30 s repeated four times and 2 min showed comparable results. Treatment with argon alone did not lead to visible reduction of bacterial growth.

Conclusion

Argon cold atmospheric plasma demonstrated a potent in vitro antimicrobial effect on P. aeruginosa, S. pseudintermedius and Str. canis strains with the latter showing the highest sensitivity.
Hybrid Sparrow Search-Exponential Distribution Optimization with Differential Evolution for Parameter Prediction of Solar Photovoltaic Models
Authors: Abd El-Mageed Amr A. , Al-Hamadi Ayoub , Bakheet Samy , Abd El-Rahiem Asmaa H.
Published: January 2024

It is difficult to determine unknown solar cell and photovoltaic (PV) module parameters owing to the nonlinearity of the characteristic current–voltage (I-V) curve. Despite this, precise parameter estimation is necessary due to the substantial effect parameters have on the efficacy of the PV system with respect to current and energy results. The problem’s characteristics make the handling of algorithms susceptible to local optima and resource-intensive processing. To effectively extract PV model parameter values, an improved hybrid Sparrow Search Algorithm (SSA) with Exponential Distribution Optimization (EDO) based on the Differential Evolution (DE) technique and the bound-constraint modification procedure, called ISSAEDO, is presented in this article. The hybrid strategy utilizes EDO to improve global exploration and SSA to effectively explore the solution space, while DE facilitates local search to improve parameter estimations. The proposed method is compared to standard optimization methods using solar PV system data to demonstrate its effectiveness and speed in obtaining PV model parameters such as the single diode model (SDM) and the double diode model (DDM). The results indicate that the hybrid technique is a viable instrument for enhancing solar PV system design and performance analysis because it can predict PV model parameters accurately.
A Pre-Trial Study to Identify Species of Origin in Halloumi Cheese Utilising Chemometrics with Near-Infrared and Hyperspectral Imaging Technologies
Authors: Tarapoulouzi Maria , Logan Natasha , Hardy Mike , Montgomery Holly , Haughey Simon A. , Elliott Christopher T. , Theocharis Charis R.
Published: January 2024

Halloumi cheese has recently gained a Protected Designation of Origin (PDO) indicator, which is related to the place (Cyprus) in which halloumi cheese is made. The PDO label is linked with several requirements, e.g., milk species, quantities, etc.; thus, it is important to study this product regarding authenticity. The utility of using two spectroscopic techniques, hyperspectral imaging (HSI) (400–1000 nm) and conventional near-infrared spectroscopy (NIR) (800–2500 nm) were assessed for the discrimination of 17 Cypriot halloumi cheese types, which could be categorized as of cow or goat–sheep origin. The aim of this study was to obtain spectral information for halloumi cheese using other promising infrared and imaging spectroscopic techniques as a comparison to a previously acquired mid-infrared (MIR) spectroscopy dataset. NIR and HSI are both fast and easy techniques in application, both of which provide significant information in food analysis. Chemometric analysis was crucial for interpreting the spectroscopic data by applying the unsupervised methods: principal component analysis (PCA) and hierarchical cluster analysis (HCA). The HSI model was found to be based intuitively on the appearance of cheese samples after freeze-drying (e.g., color; yellow/white, and texture; oily/dry), while the NIR grouping of samples was determined to be based on composition, mainly fat, protein and lactose content of the cheese samples. The HSI model returned distinct clusters of the two halloumi cheese types, cow and goat–sheep origin, with one outlier (16/17 accuracy; 94%), while the NIR model proved less accurate (13/17; 76%).
Voltage and Overpotential Prediction of Vanadium Redox Flow Batteries with Artificial Neural Networks
Authors: Martínez-López Joseba , Portal-Porras Koldo , Fernández-Gamiz Unai , Sánchez-Díez Eduardo , Olarte Javier , Jonsson Isak
Published: January 2024

This article explores the novel application of a trained artificial neural network (ANN) in the prediction of vanadium redox flow battery behaviour and compares its performance with that of a two-dimensional numerical model. The aim is to evaluate the capability of two ANNs, one for predicting the cell potential and one for the overpotential under various operating conditions. The two-dimensional model, previously validated with experimental data, was used to generate data to train and test the ANNs. The results show that the first ANN precisely predicts the cell voltage under different states of charge and current density conditions in both the charge and discharge modes. The second ANN, which is responsible for the overpotential calculation, can accurately predict the overpotential across the cell domains, with the lowest confidence near high-gradient areas such as the electrode membrane and domain boundaries. Furthermore, the computational time is substantially reduced, making ANNs a suitable option for the fast understanding and optimisation of VRFBs.
Facile Fabrication of Porous MoSe2/Carbon Microspheres via the Aerosol Process as Anode Materials in Potassium-Ion Batteries
Authors: Jo Du Yeol , Park Seung-Keun
Published: January 2024

Recently, potassium-ion batteries (KIBs) have attracted significant interest due to a number of factors, including the growing demand for energy and limited lithium resources. However, their practical use is hampered by poor cycling stability due to the large size of K+. Therefore, it is critical to develop a structural design that effectively suppresses large volume changes. This study presents a simple method of using a salt template to fabricate porous microspheres (p-MoSe2@C MS) of MoSe2 and a carbon matrix as anode materials in KIBs. These microspheres have a distinct porous design, with uniformly distributed MoSe2 nanocrystals embedded in the carbon matrix to prevent MoSe2 overgrowth due to material diffusion during heat treatment. The manufacturing process combined one-step spray drying with recyclable NaCl as a hard template. Through a two-step thermal process under an inert atmosphere, the initial dextrin, NaCl, and Mo salt microspheres were converted into a p-MoSe2@N MS composite. The carbon structure derived from the dextrin maintained the shape of the microspheres when NaCl was removed, ensuring no overgrowth of MoSe2. This well-designed porous structure improves the interaction with the electrolyte, facilitating the transport of ions and electrons and reducing the K+ diffusion distances. In addition, the porous carbon structure accommodates large volume changes during cycling and maintains its structural strength. As a result, p-MoSe2@C MS composite exhibits superior electrochemical properties, with remarkable capacity, long-term cycling stability (193 mA h g−1 after 500 cycles at 2.0 A g−1), and rate capability.
Mice Generated with Induced Pluripotent Stem Cells Derived from Mucosal-Associated Invariant T Cells
Authors: Sugimoto Chie , Fujita Hiroyoshi , Wakao Hiroshi
Published: January 2024

The function of mucosal-associated invariant T (MAIT) cells, a burgeoning member of innate-like T cells abundant in humans and implicated in many diseases, remains obscure. To explore this, mice with a rearranged T cell receptor (TCR) α or β locus, specific for MAIT cells, were generated via induced pluripotent stem cells derived from MAIT cells and were designated Vα19 and Vβ8 mice, respectively. Both groups of mice expressed large numbers of MAIT cells. The MAIT cells from these mice were activated by cytokines and an agonist to produce IFN-γ and IL-17. While Vβ8 mice showed resistance in a cancer metastasis model, Vα19 mice did not. Adoptive transfer of MAIT cells from the latter into the control mice, however, recapitulated the resistance. These mice present an implication for understanding the role of MAIT cells in health and disease and in developing treatments for the plethora of diseases in which MAIT cells are implicated.
Pelvic Exenteration for Recurrent Vulvar Cancer: A Retrospective Study
Authors: Classen-von Spee Sabrina , Baransi Saher , Fix Nando , Rawert Friederike , Luengas-Würzinger Verónica , Lippert Ruth , Bonin-Hennig Michelle , Mallmann Peter , Lampe Björn
Published: January 2024

Pelvic exenteration (PE) is one of the most radical surgical approaches. In earlier times, PE was associated with high morbidity and mortality. Nowadays, due to improved selection of suitable patients, perioperative settings, and postoperative care, patients’ outcomes have been optimized. To investigate patients’ outcomes and identify possible influencing clinical and histopathological factors, we analysed 17 patients with recurrent vulvar cancer who underwent PE in our department between 2007 and 2022. The median age was 64.9 years, with a difference of 40 years between the youngest and the oldest patient (41 vs. 81 years). The mean overall survival time was 55.7 months; the longest survival time reached up to 164 months. The achievement of complete cytoreduction (p = 0.02), the indication for surgery (curative vs. palliative), and the presence of distant metastases (both p = 0.01) showed a significant impact on overall survival. The presence of lymphatic metastases (p = 0.11) seems to have an influence on overall survival (OS) time. Major complications appeared in 35% of the patients. Our results support the existing data for PE in cases of recurrent vulvar cancer; for a group of selected patients, PE is a treatment option with good overall survival times and acceptable morbidity.
IGH::NSD2 Fusion Gene Transcript as Measurable Residual Disease Marker in Multiple Myeloma
Authors: Bors András , Kozma András , Tomán Ágnes , Őrfi Zoltán , Kondor Nóra , Tasnády Szabolcs , Vályi-Nagy István , Reményi Péter , Mikala Gábor , Andrikovics Hajnalka
Published: January 2024

Multiple myeloma (MM) is the second most common hematological malignancy. Approximately 15% of MM patients are affected by the t(4;14) translocation resulting in the IGH::NSD2 fusion transcript. Breakage occurs in three major breakpoint regions within the NSD2 gene (MB4-1, MB4-2, and MB4-3), where MB4-1 leads to the production of full-length protein, while truncated proteins are expressed in the other two cases. Measurable residual disease (MRD) has been conclusively established as a crucial prognostic factor in MM. The IGH::NSD2 fusion transcript can serve as a sensitive MRD marker. Using bone marrow (BM) and peripheral blood (PB) samples from 111 patients, we developed a highly sensitive quantitative real-time PCR (qPCR) and digital PCR (dPCR) system capable of detecting fusion mRNAs with a sensitivity of up to 1:100,000. PB samples exhibited sensitivity three orders of magnitude lower compared to BM samples. Patients with an MB4-2 breakpoint demonstrated significantly reduced overall survival (p = 0.003). Our novel method offers a simple and sensitive means for detecting MRD in a substantial proportion of MM patients. Monitoring may be carried out even from PB samples. The literature lacks consensus regarding survival outcomes among patients with different NSD2 breakpoints. Our data align with previous findings indicating that patients with the MB4-2 breakpoint type tend to exhibit unfavorable overall survival.
Neglected Anatomical Areas in Ovarian Cancer: Significance for Optimal Debulking Surgery
Authors: Kostov Stoyan , Selçuk Ilker , Watrowski Rafał , Dineva Svetla , Kornovski Yavor , Slavchev Stanislav , Ivanova Yonka , Yordanov Angel
Published: January 2024

Ovarian cancer (OC), the most lethal gynecological malignancy, usually presents in advanced stages. Characterized by peritoneal and lymphatic dissemination, OC necessitates a complex surgical approach usually involving the upper abdomen with the aim of achieving optimal cytoreduction without visible macroscopic disease (R0). Failures in optimal cytoreduction, essential for prognosis, often stem from overlooking anatomical neglected sites that harbor residual tumor. Concealed OC metastases may be found in anatomical locations such as the omental bursa; Morison’s pouch; the base of the round ligament and hepatic bridge; the splenic hilum; and suprarenal, retrocrural, cardiophrenic and inguinal lymph nodes. Hence, mastery of anatomy is crucial, given the necessity for maneuvers like liver mobilization, diaphragmatic peritonectomy and splenectomy, as well as dissection of suprarenal, celiac, and cardiophrenic lymph nodes in most cases. This article provides a meticulous anatomical description of neglected anatomical areas during OC surgery and describes surgical steps essential for the dissection of these “neglected” areas. This knowledge should equip clinicians with the tools needed for safe and complete cytoreduction in OC patients.
Study of the Resistance of Lithium-Containing Ceramics to Helium Swelling
Authors: Kozlovskiy Artem L. , Shlimas Dmitriy I. , Borgekov Daryn B. , Zdorovets Maxim V.
Published: January 2024

The paper presents the results of studies of the resistance of lithium ceramics to helium swelling during its accumulation in the structure of the near-surface layer, and the identification of the three types of lithium ceramics most resistant to radiation degradation: Li4SiO4, Li2TiO3, and Li2ZrO3. The simulation of helium swelling under high-dose irradiation was carried out by irradiation with He2+ ions with fluences of 1 × 1016 ion/cm2–5 × 1017 ion/cm2, which allows for simulating the implanted helium accumulation with a high concentration in the damaged surface layer (about 500 nm thick). The samples were irradiated at a temperature of 1000 K, the choice of which was determined by the possibility of simulating radiation damage as close as possible to real operating conditions. Such accumulation can result in the formation of gas-filled bubbles. Through the application of X-ray phase analysis, indentation testing, and thermophysical parameter assessments, it was ascertained that among the three ceramic types, Li4SiO4 ceramics exhibit the highest resistance to helium-induced swelling. These ceramics experienced less significant alterations in their properties compared to the other two types. An analysis of the correlation between the structural and strength parameters of lithium-containing ceramics revealed that the most significant changes occur when the volumetric swelling of the crystal lattice becomes the dominant factor in structural alterations. This phenomenon is manifested as an accelerated degradation of strength characteristics, exceeding 10%. At the same time, analysis of these alterations in the stability of thermophysical parameters to the accumulation of structural distortions revealed that, regardless of the type of ceramics, the degradation of thermophysical properties is most pronounced under high-dose irradiation (above 1017 ion/cm2).
Phosphorylation Codes in IRS-1 and IRS-2 Are Associated with the Activation/Inhibition of Insulin Canonical Signaling Pathways
Authors: Martínez Báez Anabel , Ayala Guadalupe , Pedroza-Saavedra Adolfo , González-Sánchez Hilda M. , Chihu Amparan Lilia
Published: January 2024

Insulin receptor substrates 1 and 2 (IRS-1 and IRS-2) are signaling adaptor proteins that participate in canonical pathways, where insulin cascade activation occurs, as well as in non-canonical pathways, in which phosphorylation of substrates is carried out by a diverse array of receptors including integrins, cytokines, steroid hormones, and others. IRS proteins are subject to a spectrum of post-translational modifications essential for their activation, encompassing phosphorylation events in distinct tyrosine, serine, and threonine residues. Tyrosine residue phosphorylation is intricately linked to the activation of the insulin receptor cascade and its interaction with SH2 domains within a spectrum of proteins, including PI3K. Conversely, serine residue phosphorylation assumes a different function, serving to attenuate the effects of insulin. In this review, we have identified over 50 serine residues within IRS-1 that have been reported to undergo phosphorylation orchestrated by a spectrum of kinases, thereby engendering the activation or inhibition of different signaling pathways. Furthermore, we delineate the phosphorylation of over 10 distinct tyrosine residues at IRS-1 or IRS-2 in response to insulin, a process essential for signal transduction and the subsequent activation of PI3K.
Effectiveness of ChatGPT in Coding: A Comparative Analysis of Popular Large Language Models
Authors: Coello Carlos Eduardo Andino , Alimam Mohammed Nazeh , Kouatly Rand
Published: January 2024

This study explores the effectiveness and efficiency of the popular OpenAI model ChatGPT, powered by GPT-3.5 and GPT-4, in programming tasks to understand its impact on programming and potentially software development. To measure the performance of these models, a quantitative approach was employed using the Mostly Basic Python Problems (MBPP) dataset. In addition to the direct assessment of GPT-3.5 and GPT-4, a comparative analysis involving other popular large language models in the AI landscape, notably Google’s Bard and Anthropic’s Claude, was conducted to measure and compare their proficiency in the same tasks. The results highlight the strengths of ChatGPT models in programming tasks, offering valuable insights for the AI community, specifically for developers and researchers. As the popularity of artificial intelligence increases, this study serves as an early look into the field of AI-assisted programming.
Factors Contributing to the Health of 0- to 5-Year-Old Low-Birth-Weight Children in the United States: Application of the Multiple Disadvantage Model
Authors: Cheng Tyrone C. , Lo Celia C.
Published: January 2024

This secondary data analysis of 1731 low-birth-weight children and their parents in the United States investigated children’s health and its associations with social disorganization, social structural factors, social relationships, health/mental health, and access to health insurance/services. The study drew on data from the 2021 National Survey of Children’s Health. Logistic regression yielded results showing low-birth-weight children’s excellent/very good/good health to be associated positively with parents’ education and health. In turn, child health was associated negatively with being Black, having a family income at or below the 100% federal poverty level, difficulty parenting the child, child chronic health condition(s), parent mental health, and substance use in the family. The implications of the present findings in terms of interventions promoting maternal and child health as well as participation in government assistance programs for low-income families are discussed.
Effects of Ghrelin on Plasminogen Activator Activity in Human Umbilical Vein Endothelial Cells
Authors: Fiacco Elisabetta , Notaristefano Giovanna , Tropea Anna , Apa Rosanna , Canipari Rita
Published: January 2024

Ghrelin and its growth hormone secretagogue receptor (GHSR) have been found in the placenta, both in endothelial and trophoblast cells. Ghrelin has been shown to decrease blood pressure in several systems and improve endothelial function by stimulating VEGF production. Because locally increased Ghrelin was detected in the preeclamptic fetoplacental unit, we hypothesized its involvement in the fibrinolysis and vascular tone typically observed in preeclamptic patients. This study aimed to evaluate the synthesis of plasminogen activators (PAs), PA inhibitor-1 (PAI-1), and urokinase-type PA (uPA) receptor (uPAR) in human umbilical vein endothelial cells (HUVECs) since the components of the PA/plasmin system are vital players in the extracellular matrix remodeling process necessary for angiogenesis. HUVECs were treated for 24 h with increasing concentrations of Ghrelin (10−11–10−7 M) or IL-1β (0.1 ng/mL). PAs, PAI-1, and uPAR mRNAs were determined by real-time PCR and PA activity was determined by casein underlay. We demonstrated an increase in uPA, tissue-type PA (tPA), and uPAR mRNA; a reduction in PAI-1 mRNA in HUVECs treated with Ghrelin; and an increase in total uPA activity. In conclusion, our results suggest a potential compensatory physiological mechanism for Ghrelin in response to the maternal endothelial dysfunction observed in the preeclamptic fetoplacental unit.
A Novel Method of Boreal Zone Reforestation/Afforestation Estimation Using PALSAR-1,2 and Landsat-5,8 Data
Authors: Bondur Valery , Chimitdorzhiev Tumen , Kirbizhekova Irina , Dmitriev Aleksey
Published: January 2024

Nowadays, global remote sensing studies of tropical forest parameters are relevant for assessing carbon sequestration, whereas boreal forests receive little attention. This is due to the current idea that forests with greater aboveground biomass absorb more carbon. However, new research indicates that rapidly growing young forests take up more carbon than mature ones. Therefore, it is necessary to develop universal methods of remote reforestation/afforestation monitoring. The existing reforestation methods rely on the separate analysis of multispectral optical images and radar data. Here, we propose a method for analyzing the joint dynamics of NDVI (or the Normalized Burn Ratio, NBR) and the radar vegetation index (RVI) on a 2D plot for a test reforestation site. NDVI and NBR time series were derived from Landsat-5,8 data, and the RVI was derived from ALOS-1,2 and PALSAR-1,2 for 2007–2020 using the resources of Google Earth Engine. The quantitative parameters to evaluate the degree of reforestation and changes in the species composition of young trees have been suggested. The suggested method enables a more thorough evaluation of reforestation by measuring the coupled dynamics of the projective cover of young trees and aboveground biomass.
Water-Related Properties of Wood after Thermal Modification in Closed Process under Pressure in Nitrogen
Authors: Sosins Guntis , Grinins Juris , Brazdausks Prans , Zicans Janis
Published: January 2024

Silver birch (Betula pendula) and Scots pine (Pinus sylvestris) wood boards (1000 × 100 × 25 mm) were thermally modified in a 340 L pilot-scale device in nitrogen with a special focus on increasing dimensional stability and reducing hydrophilicity. The research expands our understanding of the TM process in a closed system under pressure of nitrogen and its impact on the water absorption capabilities of wood. Several thermal modification (TM) parameters were tested, including temperature (160–180 °C), maximum temperature duration (30–180 min), and TM chamber initial pressure (3–6 bar). TM wood dimensional changes, mass loss (ML), equilibrium moisture content (EMC), and anti-swelling efficiency (ASE) were determined to characterize the TM process intensity and evaluate the hydrophilicity. Birch wood exhibited a higher ML (5.9%–12%) than pine wood (2.6%–9%) after TM. TM caused a shrinkage in the tangential, radial, and total volume of both wood species. The TM birch wood ASE values varied from 22% to 69%, while the pine wood ASE was 27% to 58%. The cell wall total water capacity (CWTWC) of TM wood was greatly reduced. The EMC and volumetric swelling (VS) of TM birch and pine wood were 29% to 67% lower, respectively, at all relative humidities (65, 75, and 95%).
Locus-Specific and Stable DNA Demethylation at the H19/IGF2 ICR1 by Epigenome Editing Using a dCas9-SunTag System and the Catalytic Domain of TET1
Authors: Albrecht Claudia , Rajaram Nivethika , Broche Julian , Bashtrykov Pavel , Jeltsch Albert
Published: January 2024

DNA methylation is critically involved in the regulation of chromatin states and cell-type-specific gene expression. The exclusive expression of imprinted genes from either the maternal or the paternal allele is regulated by allele-specific DNA methylation at imprinting control regions (ICRs). Aberrant DNA hyper- or hypomethylation at the ICR1 of the H19/IGF2 imprinting locus is characteristic for the imprinting disorders Beckwith–Wiedemann syndrome (BWS) and Silver–Russell syndrome (SRS), respectively. In this paper, we performed epigenome editing to induce targeted DNA demethylation at ICR1 in HEK293 cells using dCas9-SunTag and the catalytic domain of TET1. 5-methylcytosine (5mC) levels at the target locus were reduced up to 90% and, 27 days after transient transfection, >60% demethylation was still observed. Consistent with the stable demethylation of CTCF-binding sites within the ICR1, the occupancy of the DNA methylation-sensitive insulator CTCF protein increased by >2-fold throughout the 27 days. Additionally, the H19 expression was increased by 2-fold stably, while IGF2 was repressed though only transiently. Our data illustrate the ability of epigenome editing to implement long-term changes in DNA methylation at imprinting control regions after a single transient treatment, potentially paving the way for therapeutic epigenome editing approaches in the treatment of imprinting disorders.
Melatonin Promotes Accumulation of Resveratrol and Its Derivatives through Upregulation of PAL, 4CL, C4H, and STS in Grape Seeds
Authors: Gao Shiwei , Wei Dezheng , Pan Dandan , Wang Fei , Kang Hui , Yao Yuxin
Published: January 2024

Melatonin’s role in regulating the biosynthesis of phenolic compounds, particularly stilbenes, remains unknown in grape seeds. In this study, widely targeted metabolomics analysis revealed that 82 phenolic compounds were differentially accumulated in grape seeds from the berries treated with melatonin. The accumulation of resveratrol and its 11 derivatives was largely increased in melatonin-treated berry seeds. Additionally, melatonin treatment of preveraison grape berries increased the resveratrol content in seeds during berry ripening. The results of RNA-Seq showed that a total of 399 genes were differentially expressed, which were mainly involved in the plant hormone signal transduction, metabolic, and biosynthesis of secondary metabolites pathways, in melatonin-treated berry seeds compared to control seeds. Additionally, five PALs, four 4CLs, one C4H, and four STSs were elucidated to be induced by melatonin in seeds at different time points after treatment. Therefore, melatonin promotes resveratrol accumulation and its derivatives, probably by upregulating the genes in the phenylpropanoid pathway, which provides precursors for resveratrol biosynthesis, and the STS genes in grape seeds.