bluuebunny/crossref_metadata_2025 huggingface dataset:
Associations between reading and writing postures and myopia among school students in Ningbo, China
Authors: Jiang Danjie , Shi Bijun , Gao Hua , Guo Yanbo , Zhou Shaoying , Zhang Yan
Published: August 2022

Background

We conducted this study to investigate the prevalence of myopia among school students in Ningbo and to explore the associations between reading and writing postures and myopia.

Methods

A population-based and cross-sectional study was conducted, and 3,256 school students aged 8–19 years were recruited. Each enrolled subject was assessed for uncorrected distance visual acuity (UDVA) using a standard logarithmic visual acuity E-chart and a non-cycloplegic autorefraction examination. Self-administered questionnaires were used to investigate myopia-related reading and writing postures and behavioral habits among school students.

Results

The prevalence of myopia among primary school, middle school, and high school students was 61.49, 81.43, and 89.72%, respectively. Regarding the associations between reading and writing postures and myopia, we identified that a reading distance >33 cm is a protective factor for myopia in female students [odds ratio (OR) = 0.31, 95% confidence interval (CI) = 0.15–0.64], in both primary school (OR = 0.55, 95% CI = 0.30–0.99) and middle school (OR = 0.37, 95% CI = 0.15–0.90).

Conclusions

A reading distance >33 cm can be used as an additional measure to prevent and control myopia. Proper postural measures for reading and writing may have educational and public health benefits.
Confidence interval estimation for vaccine efficacy against COVID-19
Authors: Wei Qinyu , Wang Peng , Yin Ping
Published: August 2022

This article focuses on the construction of a confidence interval for vaccine efficacy against contagious coronavirus disease-2019 (COVID-19) in a fixed number of events design. Five different approaches are presented, and their performance is investigated in terms of the two-sided coverage probability, non-coverage probability at the lower tail, and expected confidence interval width. Furthermore, the effect of under-sensitivity of diagnosis tests on vaccine efficacy estimation was evaluated. Except for the exact conditional method, the non-coverage probability of the remaining methods may exceed the nominal significance level, e.g., 5%, even for a large number of total confirmed COVID-19 cases. The narrower confidence interval width from the Bayesian, approximate Poisson, and mid-P methods are on the cost of increased instability of coverage probability. When the sensitivity of diagnosis test in the vaccine group is lower than that in the placebo group, the reported vaccine efficacy tends to be overly optimistic. The exact conditional method is preferable to other methods in COVID-19 vaccine efficacy trials when the total number of cases reaches 60; otherwise, mid-p method can be used to obtain a narrower interval width.
Association between social capital and depression among older adults of different genders: Evidence from Hangzhou, China
Authors: Zhou Siyu , Li Kai , Ogihara Atsushi , Wang Xiaohe
Published: August 2022

In China, it is critical to help older adults cope with depression due to the emerging impacts of factors such as increased life expectancy and the “one-child” family planning policy. Meanwhile, differences in retirement age have different effects on health in older adults of different gender. The relationship of gender differences in social capital and depression across the elderly population was unclear. Focusing on this demographic, this study conducted a telephone survey to explore the relationship between social capital and depression. Referring to electronic medical records, we randomly selected 1,042 elderly respondents (426 men, 616 women) from four areas in Hangzhou. We used social capital measurements and the Geriatric Depression Scale (GDS-15) to assess social capital and depression, respectively, then employed a multivariate logistic regression and structural equation modeling to examine the associations between factors, along with a consideration of gender. This study was discovered that differences in both income and morbidity contributed to differences in social capital and depression. In our sample of elderly respondents, we also found gender-based differences in cognitive and structural social capital. Compared to men, women were more likely to attain higher social capital and less likely to develop depression. At the same time, social networking and social engagement had negative impacts on depression in women, which was not the case for men. We found that lower reciprocity (men and women), social work (men), and trust (women) indicated higher risks of depression. Reciprocity and social networks were significantly and negatively correlated with depression among male respondents; in the male model, factors of trust, reciprocity, and social participation had positive effects on reducing the risk of depression, while social networks had a negative effect. For elderly persons, these findings suggest that mental health is affected by differences in social capital caused by policy differences and cultural differences caused by gender differences.
Cognitive functions and anxiety among blue-collar workers in hospitals during COVID-19 pandemic
Authors: Mohammadi Abbas , Ibrahimi Ghavamabadi Leila , Silavi Maryam , Dehaghi Behzad Fouladi
Published: August 2022

Background

The rapid spread of COVID-19 poses a major threat to public health worldwide. Hospital blue-collar workers, like other health care workers, may be under severe physical and psychological stress. This psychological distress is mainly described as symptoms of anxiety and depression, stress and cognitive function. This study aimed to investigate the effects of anxiety on cognitive function among laundry and sterilization center workers in 4 hospitals during COVID-19 pandemic.

Method

This study has a cross-sectional design and it was conducted among health service workers who were occupied in health facilities (laundry and sterilization center) in 4 hospitals. In the present study, two questionnaires and a test were used to collect the data included a general health questionnaire (GHQ-28), a health anxiety index questionnaire (HAI-18) and a cognitive function test. Descriptive statistics and Spearman correlation coefficient by SPSS version 19 were used.

Results

The results showed that the overall score of coronavirus anxiety in male and female workers was 38.72 ± 5.94 and 40.92 ± 4.11, respectively. The correlation between auditory attention and coronavirus anxiety is moderate and has a negative trend (P = 0.050, n = 50, and r = −0.315). Workers with higher anxiety expressed lower auditory attention. Also, correlation between visual attention and coronavirus anxiety was weak and negative (P = 0.032, n = 50, and r = −0.179).

Conclusion

This study revealed that cognitive and psychological aspects of mental health can be affected by COVID-19 exposure and its due anxiety in blue-collar workers in hospitals. These findings indicate that purposeful supportive interventions should be implemented to promote workers' health and cognitive function.
A qualitative analysis of free text comments of participants from a massive open online mindfulness course
Authors: Neate Sandra L. , Reece Jeanette C. , Hassed Craig , Chambers Richard , Connaughton Sherelle , Nag Nupur
Published: August 2022

Introduction

Mindfulness-based interventions are associated with improved health and wellbeing. Online mindfulness interventions offer potential scalability and cost advantages over face-to-face interventions. However, little is known about the experiences of learners, what they identify as being helpful to developing a practice of mindfulness and what outcomes they experience from undertaking an online mindfulness program.

Methods

The Mindfulness for Wellbeing and Peak Performance Massive Open Online Course is a 4-week mindfulness program which includes psychoeducation, mindfulness meditation, applications and moderated discussion forums. Of the 3,335 participants who completed the March 2020 course, 527 (16%) responded to the final forum which invited participants to describe the highlights of the course. In order to enhance understanding of participant experiences and perceived outcomes of undertaking the course, a qualitative analysis of these free text comments was conducted using reflexive thematic analysis.

Results

Two overarching themes were identified: (1) internal mechanisms of developing mindfulness (subthemes: paying attention to the present moment, learning to let go and find acceptance, cultivating an attitude of gentleness, and learning through a sense of belonging) and (2) the translation of mindfulness into daily living (subthemes: mindfulness being a support to mental wellbeing, learning to deal with uncertainty and adversity, living a more conscious life, a greater connection with self and others and channeling attention into productivity).

Discussion

The themes and subthemes provided insights into the mechanisms learners used to develop mindfulness and how they translated mindfulness into their lives in a variety of beneficial ways. This understanding of learners' experiences could inform delivery of future online mindfulness interventions.
Cost-effectiveness analysis of colonoscopy and fecal immunochemical testing for colorectal cancer screening in China
Authors: Ren Yinan , Zhao Mingye , Zhou Dachuang , Xing Qian , Gong Fangfang , Tang Wenxi
Published: August 2022

Objective

This study aimed to evaluate the cost-effectiveness of the colorectal cancer screening in China, and that when the screening was implemented in a specific region.

Methods

A 13-state Markov model was established to compare four screening protocols, including annual fecal immunochemical testing (FIT1), biennial fecal immunochemical testing (FIT2), electronic colonoscopy every 10 years (e-CSPY10), and electronic colonoscopy every 5 years (e-CSPY5), with no screening from the perspective of Chinese healthcare system. The model simulated the health states of a cohort of 100,000 average-risk individuals aging from 50 to 75. Additionally, scenarios including the implementation in a specific region, starting from 40, and incompletely successful treatment of cancer were also analyzed.

Results

Annual and biennial FIT could save 8.13USD (US Dollar) and 44.96USD per person, and increase 0.0705QALYs (Quality-Adjusted Life Years) and 0.2341 QALYs compared with no screening, respectively. Annual FIT could decrease costs by 36.81USD per person and increase 0.1637 QALYs in comparison to biennial FIT. The results showed that both annual and biennial FIT for screening were dominant over no screening, and annual FIT was dominant over biennial FIT. The ICER (Incremental Cost-Effectiveness Ratio) for e-CSPY10 were 1183.51USD/QALY and 536.66USD/QALY compared with FIT1 and FIT2. The ICER for e-CSPY5 were 1158.16USD/QALY and 770.85USD/QALY compared with FIT1 and FIT2. And the ICER for e-CSPY5 relative to e-CSPY10 was 358.71USD/QALY. All the ICER values were lower than the economic threshold of 2021 Chinese GDP (Gross Domestic Product) per capita in 2021(12554.42USD).

Conclusions

It is worthwhile to popularize CRC screening in mainland China, as FIT always saving costs and colonoscopy is cost-effective. Regions with high income can take electronic colonoscopy every 10 years, or even every 5 years into consideration when determining the specific strategies.
Current knowledge and “myths” about celiac disease among physicians in the Republic of Kazakhstan: A countrywide cross-sectional study
Authors: Kozhakhmetova Aizhan , Aidossov Serzhan , Kapassova Aissulu , Borsoldayeva Karlygash
Published: August 2022

Background

Celiac disease (CD) is a common genetically predisposed autoimmune condition affecting the gut and other organs. Disease awareness is one of the key components of early case identification. This study aimed to assess awareness about CD among primary care physicians, who are the front-liners in suspecting the diagnosis, and other medical specialists.

Methods and findings

The questionnaire for this survey-based study was created based on the latest international guidelines on CD and included a consent form, 5 general questions (age, gender, etc.), and 10 specific questions concerning CD. Overall, 232 respondents from 13 country provinces (out of 14) and two republican cities were recruited for this study. Of them, 110 (47.4%) were primary care physicians and 122 (52.6%) other medical specialists, including 10 (4.3%) gastroenterologists. A scoring system was used to classify the level of awareness of participants into 3 categories, namely, poor, fair, and good. Analysis of responses revealed poor awareness in 59.4% of physicians, associated with work in republican/province/district/rural/village hospitals (p = 0.004), male gender (p = 0.006), and age of 40–50 years (p = 0.02). The most common “myths” about CD were the following: “symptoms are always obvious in children” or “in adults” (92.5 or 88.4% of respondents, respectively); “genetic mutation HLA DQ2/DQ8 causes the development of CD in all carriers of the mutation” (51.3%); “CD is a disease of children only” (12.5%); and “is triggered by dairy products” (8.6%). Genotyping of HLA DQ genes has been recommended in case of CD suspicion by every third respondent and was advocated as a “golden standard” confirmatory test by every fifth respondent. A quarter of respondents revealed their incorrect treatment strategies: gluten-free diet for 1 month, dairy-free diet, Helicobacter pylori eradication therapy, or responded that did not know how to treat. Overall, 93.5% of respondents expressed intention to learn more about CD, while the rest 6.5% thought that they knew enough, although their knowledge was poor.

Conclusion

This study revealed a poor level of awareness among physicians in Kazakhstan and identified common misconceptions about CD, which potentially could lead to incorrect application of diagnostic tests, delay in diagnosis, and inefficient treatment. Development and implementation of educational programs as well as promotion of self-learning would increase awareness and unravel misconceptions.
The effects of environmental tax reform on urban air pollution: A quasi-natural experiment based on the Environmental Protection Tax Law
Authors: Guo Bingnan , Wang Yu , Feng Yu , Liang Chunyan , Tang Li , Yao Xiafei , Hu Feng
Published: August 2022

Air pollution significantly impacts sustainable development and public health. Taking the implementation of China's Environmental Protection Tax Law in China as a quasi-natural experiment, this paper employs the difference-in-differences (DID) and spatial DID models to evaluate the effects of environmental tax reform on urban air pollution. The findings are as follows. (1) Environmental tax reform can significantly reduce urban air pollution, and a series of robustness tests have also been conducted to provide further evidence. (2) Green technology innovation and industrial structure upgrading from a vital transmission mechanism for environmental tax reform to improve air quality. (3) Environmental tax reform significantly inhibits urban air pollution in cities located north of the Qinling-Huaihe line and big cities. (4) Moreover, environmental tax reform not only promotes the improvement of local air quality but also has a significant negative spatial spillover effect, reducing air pollution in neighboring cities. The research conclusions provide theoretical support and policy suggestions for promoting sustainable economic development, rationally optimizing environmental protection tax policies and improving urban air quality.
Why government supported smart city initiatives fail: Examining community risk and benefit agreements as a missing link to accountability for equity-seeking groups
Authors: Sengupta Ushnish , Sengupta Ulysses
Published: August 2022

This paper utilizes concepts from a critical social justice discourse on smart cities to identify factors behind resistance to new smart city initiatives from equity-seeking groups. The broader critical discourse is examined based on relevance to the eventual failure of the initiatives selected as case studies. It highlights institutional failure within government-supported initiatives due to the lack of consideration given to equitable distribution of risks and formal accountability mechanisms. It describes outcomes surrounding smart cities in which the benefits accrue to some groups within the city while risks increase for other groups. Finally, we examine the integration of “risk” as an adaptation to the existing practical mechanism of Community Benefit Agreements, for use of this framework to support value sensitive design approaches in future smart city initiatives.
Toward a multidimensional indicator of resident-oriented sustainable development: The case of slum areas in Mumbai
Authors: Kitsuki Akinori , Managi Shunsuke
Published: August 2022

This paper proposes a framework for weighting priority for the multidimensional domains of slum development from the viewpoint of residents. The weights estimated by our framework can be interpreted as marginal utility, and multiplying satisfaction scores in each domain of development by these weights yields residents' utility from slum development. The proposed approach is carried out by accessing residents' needs for slum development in Mumbai, India, where more than 5.2 million residents live in slum areas. Using the dataset obtained from a questionnaire survey that we conducted with slum residents in March 2019, we estimate marginal utilities for 23 domains of slum development. The results show that (1) slum residents most wish to improve development projects about human capital (health and education), electricity, and drinking water, even if they have already been highly satisfied, (2) they feel the least satisfaction with public toilets and place high priority on projects involving public toilets, (3) the sewage system has low priority with low satisfaction, but this priority increases as slum residents become better off, (4) the projects on social development and protection are highly satisfied and generally ranked low in terms of marginal utilities, and (5) air pollution and working conditions are also concerns of slum residents, especially as these residents become better off. It is expected that the framework used in this paper can be used to extract the problems of urban development and to track the progress of development plans from the viewpoint of residents.
Early enteric and hepatic responses to ingestion of polystyrene nanospheres from water in C57BL/6 mice
Authors: Szule Joseph A. , Curtis Lawrence R. , Sharpton Thomas J. , Löhr Christiane V. , Brander Susanne M. , Harper Stacey L. , Pennington Jamie M. , Hutton Sara J. , Sieler Michael J. , Kasschau Kristin D.
Published: August 2022

Drinking water is one of numerous sources of human exposure to microscale and nanoscale plastic particles. Here, using a mouse model, we tested enteric and hepatic cellular responses to nanoplastic ingestion. At 1.5 or 25.5 h after an oral dose of 70 mg polystyrene nanospheres (PSNS)/kg (nominal diameters of 20 and 200 nm) in aqueous suspension female mice exhibit no overt signs of toxicity. Routine histopathology on small intestine and liver reveals no acute toxicity. Immunohistochemistry detects an increase in the number of enterocytes with cleaved caspase-3 (active form) after PSNS exposure (p ≤ 0.05) indicating progression toward lytic cell death via a proinflammatory pathway. This is not evident in liver after PSNS exposure. Transmission electron microscopy detects lytic cell death in enterocytes at 25.5 h after 200 nm PSNS exposure. Putative endosomes in liver appear to sequester 20 and 200 nm particles 25.5 h after exposure. Both 20 and 200 nm PSNS appear in putative perinuclear autolysosomes 25.5 h after treatment. No significant changes in gene expression in the small intestine or liver 25.5 h were observed after dosing, but there was a trend toward altered expression of cyp1b1 in the liver. Analysis of the fecal microbiome shows loss of diversity after exposure to both 20 and 200 nm particles after 25.5 h. Taken together, these results suggest risk from ingestion of nanoscale plastic particles from drinking water, which deserves systematic investigation.
Towards coupling full-disk and active region-based flare prediction for operational space weather forecasting
Authors: Pandey Chetraj , Ji Anli , Angryk Rafal A. , Georgoulis Manolis K. , Aydin Berkay
Published: August 2022

Solar flare prediction is a central problem in space weather forecasting and has captivated the attention of a wide spectrum of researchers due to recent advances in both remote sensing as well as machine learning and deep learning approaches. The experimental findings based on both machine and deep learning models reveal significant performance improvements for task specific datasets. Along with building models, the practice of deploying such models to production environments under operational settings is a more complex and often time-consuming process which is often not addressed directly in research settings. We present a set of new heuristic approaches to train and deploy an operational solar flare prediction system for ≥M1.0-class flares with two prediction modes: full-disk and active region-based. In full-disk mode, predictions are performed on full-disk line-of-sight magnetograms using deep learning models whereas in active region-based models, predictions are issued for each active region individually using multivariate time series data instances. The outputs from individual active region forecasts and full-disk predictors are combined to a final full-disk prediction result with a meta-model. We utilized an equal weighted average ensemble of two base learners’ flare probabilities as our baseline meta learner and improved the capabilities of our two base learners by training a logistic regression model. The major findings of this study are: 1) We successfully coupled two heterogeneous flare prediction models trained with different datasets and model architecture to predict a full-disk flare probability for next 24 h, 2) Our proposed ensembling model, i.e., logistic regression, improves on the predictive performance of two base learners and the baseline meta learner measured in terms of two widely used metrics True Skill Statistic (TSS) and Heidke Skill Score (HSS), and 3) Our result analysis suggests that the logistic regression-based ensemble (Meta-FP) improves on the full-disk model (base learner) by ∼9% in terms TSS and ∼10% in terms of HSS. Similarly, it improves on the AR-based model (base learner) by ∼17% and ∼20% in terms of TSS and HSS respectively. Finally, when compared to the baseline meta model, it improves on TSS by ∼10% and HSS by ∼15%.
-Omics studies of plant biology in spaceflight: A critical review of recent experiments
Authors: Hughes Ariel M. , Kiss John Z.
Published: August 2022

Researchers have been studying transcriptomic and proteomic responses of plants to ranges of reduced gravitational conditions. These include blue and red light in microgravity, circadian rhythms in microgravity, microgravity in different ecotypes, microgravity on suborbital flights, and they have using a variety of experimental equipment. Recent findings have linked microgravity and transcriptomic changes in genes relating to cell wall synthesis and modification, oxidative stress, abiotic stressors, phytohormones, sugar synthesis and metabolism, ribosomal biogenesis, and plant defense to other organisms. Although we have a better-established profile of the transcriptomic response of plants to reduced gravity, some areas of study have not yet been thoroughly investigated. The initial stages and progression of transcriptional responses to microgravity, the responses of additional plant species, and tissue-specific transcriptional responses to microgravity should all be further investigated in order to better develop our understanding of how plants react to a reduced gravity environment. In the near future, advancing technology, rapidly growing databases, and an increasing number of spaceflight opportunities will allow for more research to be conducted to address these and many other related questions in plant space biology.
Access to prairie pollen affects honey bee queen fecundity in the field and lab
Authors: St. Clair Ashley L. , Suresh Sreelakshmi , Dolezal Adam G.
Published: August 2022

Beekeepers experience high annual losses of colonies, with environmental stressors like pathogens, reduced forage, and pesticides as contributors. Some factors, like nutritional stress from reduced flower abundance or diversity, are more pronounced in agricultural landscapes where extensive farming limits pollen availability. In addition to affecting other aspects of colony health, quantity and quality of pollen available are important for colony brood production and likely for queen egg laying. While some US beekeepers report >50% of colony loss due to queen failure, the causes of poor-quality queens are poorly understood. Access to resources from native prairie habitat is suggested as a valuable late-season resource for honey bees that can reverse colony growth declines, but it is not clear how prairie forage influences queen egg laying. We hypothesized that the pollen resources present in an extensive Midwestern corn/soybean agroecosystem during the critical late season period affect honey bee queen egg laying and that access to native prairies can increase queen productivity. To test this, we designed a field experiment in Iowa, keeping colonies in either soybean or prairie landscapes during a critical period of forage dearth, and we quantified queen egg laying as well as pollen collection (quantity and species). Then, using pollen collected in the field experiments, we created representative dietary mixtures, which we fed to bees using highly controlled laboratory cages to test how consumption of these diets affected the egg laying of naive queens. In two out of three years, queens in prairies laid more eggs compared to those in soybean fields. Pollen quantity did not vary between the two landscapes, but composition of species did, and was primarily driven by collection of evening primrose (Oenothera biennis). When pollen representative of the two landscapes was fed to caged bees in the laboratory queens fed prairie pollen laid more eggs, suggesting that pollen from this landscape plays an important role in queen productivity. More work is needed to tease apart the drivers of these differences, but understanding how egg laying is regulated is useful for designing landscapes for sustainable pollinator management and can inform feeding regimes for beekeepers.
A rare cause of gastrointestinal bleeding in adults: Ileal duplication with ulceration: A case report and review of literature
Authors: Li Lingli , Li Hongping , Chen Xiaoyan , Yao Jin , Xie Rui , Wang Hong
Published: August 2022

The ileal duplication, which is a congenital anatomical abnormality of the digestive tract, can lead to the manifestation of the alimentary tract duplication in the small intestine. It is also the rare cause of gastrointestinal bleeding and usually seen in infants, but only rarely in adults. Herein, we describe a case of adult male was suffering from intermittent massive hematochezia for three years, accompanied by abdominal pain, syncope, and palpitations. However, no positive findings were found by gastroscopy and colonoscopy in other hospitals. He visited our hospital owing to the recurrent hematochezia, but re-examination by gastroscopy and colonoscopy indicated no significant abnormality, and hence small intestinal vascular malformation bleeding was considered. Therefore, double-balloon enteroscopy (DBE) examination was conducted and the results revealed a double-lumen opening in the ileum, which was 100 cm from the ileocecal valve. The blind end was observed 6 cm away from one opening along the depth direction, and an ulcer was observed on the intestinal wall of the blind segment, which was considered as an ileal duplication. The patient thereafter underwent surgery subsequently. Postoperative pathology confirmed ileal duplication and that gastrointestinal bleeding was primarily caused by ulcer hemorrhage. The patient had no discomfort after the follow-up. In this report, we have reviewed and summarized the literature to provide references for both diagnosis and treatment of ileal duplication.
Stem cell-derived exosomal transcriptomes for wound healing
Authors: Chen Guiling , Chen Hankun , Zeng Xiang , Zhu Wei
Published: August 2022

Wound healing is a complex and integrated process of the interaction of various components within the injured tissue. Accumulating evidence suggested that stem cell-derived exosomal transcriptomes could serve as key regulatory molecules in wound healing in stem cell therapy. Stem cell-derived exosomal transcriptomes mainly consist of long noncoding RNAs (lncRNAs), microRNAs (miRNAs), circular RNAs (circRNAs) and messenger RNAs (mRNAs). In this article we presented a brief introduction on the wound repair process and exosomal transcriptomes. Meanwhile, we summarized our current knowledge of the involvement of exosomal transcriptomes in physiological and pathological wound repair process including inflammation, angiogenesis, and scar formation.
Analysis of the drainage effect of different incisions for high complex anal fistula based on FLUENT hydrodynamic simulation
Authors: Zhang Jiamin , Li Xiang , Ma Jiaze , Chen Peng , Li Wanli , Hu Junjie , Li Xiaoliu , Chen Yile , Ding Kang
Published: August 2022

Purpose

The biomechanical characteristics of the trauma size and postoperative drainage of different incisions for high complex anal fistula surgery were compared by numerical simulation analysis to provide a theoretical basis for the clinical selection of minimally invasive incisions for surgery.

Methods

Using FLUENT finite element software, a typical incision finite element model was established to obtain incision areas, and the total mass outlet flow within 200 s was calculated to evaluate the drainage effect of each incision.

Results

The incisions with the largest to smallest areas were the curved, spindle, and curved plus extended groove incision, indicating that the curved plus extended groove incision caused the least damage to the perianal skin muscles. Conversely, the incisions with the largest to smallest total outlet flow were as follows: curved plus extended groove, spindle, curved, and straight incision, suggesting that the curved plus extended groove model had the best diversion effect, and the curved incision had better diversion effect than that of the straight incision.

Conclusion

The curved plus extended groove surgical incision had the smallest incision area, minimized damage to the perianal skin and muscle tissue, conformed to the concept of minimally invasive surgery, ensured adequate drainage of exudate, maintained the normal growth of granulation tissue on the wound surface, preserved the original form of the anus, and thus better protected the function of the anus. This improved the quality of life of patients requiring high complex anal fistulas.
Distal end of Double-J ureteral stent position on ureteral stent-related symptoms: A systematic review and meta-analysis
Authors: Bao Xingjun , Sun Fengze , Yao Huibao , Wang Di , Liu Hongquan , Tang Gonglin , Wang Xiaofeng , Zhou Zhongbao , Wu Jitao , Cui Yuanshan
Published: August 2022

Background

Most patients suffer from ureteral stent-related symptoms (USRS) caused by indwelling ureteral stents. Nevertheless, various medications to alleviate discomfort as well as novel stents are continually being developed, and in recent years, some researchers have believed that proper intravesical stent placement can relieve USRS.

Objective

To determine appropriate intravesical ureteral stent position may alleviate USRS.

Methods

Up to May 1, 2022, the PubMed, Embase, Scopus and Web of Science databases were thoroughly searched, and two independent reviewers included relevant studies that met the PICO (Patient, Intervention, Comparison, Outcome) criteria. Studies methodological quality were assessed by ROB2 and ROBINS-I. Ureteral stent symptom questionnaire (USSQ), international prostate symptom score (IPSS) and quality of life (QoL) was used to quantify the USRS. According to intravesical ureteral stent position, Group A was defined as the contralateral group, that is distal end of ureteral stent crossed the bladder midline, whereas Group B was classified as ipsilateral group, meaning stent end did not cross the midline.

Results

Six studies incorporating a total of 590 patients were eligible. In terms of USSQ score, the meta-analysis showed that contralateral group was associated with a significant increase in USSQ total (MD, 17.55; 95% CI, 12.04 to 23.07; P < 0.001), urinary symptoms (MD, 2.74; 95% CI, 0.48 to 5.01; P = 0.02), general health (MD, 4.04; 95% CI, 2.66 to 5.42; P < 0.001), work performance (MD, 1.36; 95% CI, 0.75 to 1.98; P < 0.001) and additional problems (MD, 0.89; 95% CI, 0.47 to 1.32; P < 0.001) scores while not associated with a significant increase in body pain (MD, 3.13; 95% CI, −0.19 to 6.44; P = 0.06) and sexual matters (MD, 1.01; 95% CI, −0.03 to 2.06; P = 0.06). As for IPSS, although no significant differences in IPSS total (MD, 2.65; 95% CI, −0.24 to 5.54; P = 0.07) or voiding symptoms (MD, −0.84; 95% CI, −3.16 to 1.48; P = 0.48) scores were found, ipsilateral group was associated with a significant decrease in storage symptoms (MD, 1.92; 95% CI, 0.91 to 2.93; P = 0.0002). Furthermore, ipsilateral group was linked to a significant decrease in QoL score (MD, 1.00; 95% CI, 0.18 to 1.82; P = 0.02).

Conclusion

This meta-analysis proven that correct intravesical stent position was critical, and patients with stents crossing the midline experienced more severe USRS than those who did not. Further high-quality randomized controlled trials are needed to corroborate our findings.
Pan-immune-inflammation value is associated with the clinical stage of colorectal cancer
Authors: Zhao HanZheng , Chen Xingyu , Zhang WenHui , Cheng Die , Lu Yanjie , Wang Cheng , Li JunHu , You LiuPing , Yu JiaYong , Guo WenLong , Li YuHong , Huang YueNan
Published: August 2022

Objective

We investigated the clinical significance of preoperative pan-immune-inflammation value (PIV) in patients with colorectal cancer (CRC).

Methods

In this retrospective study, 366 cases who underwent surgery for CRC were enrolled. Their clinical data were collected. PIV was calculated with the formula PIV = [neutrophil count (109/L)× platelet count (109/L) × monocyte count (109/L) /lymphocyte count (109/L). Patients were divided into high PIV (> median PIV) and low PIV (< median PIV) groups. The relationship between PIV and clinicopathological features of CRC was investigated. Receiver operating characteristic (ROC) curve was plotted to indicate the value of immune-inflammatory biomarkers (IIBs) in predicting the TNM stage of CRC, and the area under the curve (AUC) was calculated to evaluate the actual clinical value of IIBs. AUC > 0.5 and closer to 1 indicated the better predictive efficacy. The influencing factors of PIV in CRC were analyzed.

Results

We found that PIV was positively correlated with tumor size (r = 0.300, p < 0.05), carcinoembryonic antigen (CEA) (r = 0.214, p < 0.05) and carbohydrate antigen 125 (CA-125) (r = 0.249, p < 0.05), but negatively correlated with albumin (Alb) (r = −0.242, p < 0.05). PIV was significantly different in patients with different tumor locations (left or right), surgical methods (laparotomy versus laparoscopic surgery) (p < 0.05), and patients with different pathological T stages, N-stage and TNM stages (p < 0.05). ROC curve analysis of IIBs showed the AUC of PIV was greater than other markers when combined with CEA or carbohydrate antigen 19–9 (CA19–9). Multivariate regression analysis identified T stage, CEA, Alb, and tumor size as the independent influential factors of PIV in CRC.

Conclusion

PIV is associated with the tumor stage in patients with CRC, which may be useful in preoperative assessment of CRC.
Metataxonomic insights into the microbial ecology of farm-scale hay, grass or legume, and corn silage produced with and without inoculants
Authors: Kennang Ouamba Alexandre J. , Gagnon Mérilie , Varin Thibault , Chouinard P. Yvan , LaPointe Gisèle , Roy Denis
Published: August 2022

The microbiota of silage is a key determinant of its quality. Although commercial inoculants are often used to improve silage quality, studies to analyze their impact on the microbiota of preserved forage at farm-scale facilities are scarce. We assessed the diversity of viable bacterial communities of hay (unfermented dry forage) and grass or legume (GL) and corn (C) silage to deepen our knowledge of how inoculant addition drives microbial occurrence patterns on dairy farms. Forage samples were collected from 24 dairy farms over two sampling periods. Samples were analyzed by high-throughput sequencing and quantitative PCR after being treated with propidium monoazide to account for viable cells. We found consistent significant differences between hay and silage community structures across sampling periods. Silage was generally dominated by lactic acid bacteria (LAB), while Pantoea and Sphingomonas were the main co-dominant genera in hay. The GL silage dominated by Pediococcus, Weissella, and Bacillus was phylogenetically different from C silage enriched in Acetobacter. The use of inoculants including Lentilactobacillus buchneri either alone or in combination with Lactiplantibacillus plantarum, Lacticaseibacillus casei, Pediococcus pentosaceus, or Enterococcus faecium did not systematically prevent the occurrence of undesirable bacteria, especially when corn-based, probably because of factors that can mitigate the effect of inoculation on the microbiota. The core Lactobacillales constituted the dominant LAB in silage with up to 96% relative abundance, indicating either the ubiquity of inoculants or the high competitiveness of epiphytes. Silage chemical profiles varied inconsistently with sampling periods and the use of inoculants. Multivariate multi-table analyses allowed the identification of bacterial clusters mainly driven by moisture and magnesium content in hay, while pH, lactic, and fatty acids were the main drivers for silage. Bacterial network analyses showed considerable variations in the topological roles with the use of inoculants. These results may help evaluate the effectiveness of forage management practices implemented on dairy farms and, therefore, are useful for fine-tuning the search for new additives. Such knowledge can be used by forage makers to adjust processing routines to improve the hygienic quality, nutritional potential, and aerobic stability of preserved forage.
Social behavior in farm animals: Applying fundamental theory to improve animal welfare
Authors: Lee Victoria E. , Arnott Gareth , Turner Simon P.
Published: August 2022

A fundamental understanding of behavior is essential to improving the welfare of billions of farm animals around the world. Despite living in an environment managed by humans, farm animals are still capable of making important behavioral decisions that influence welfare. In this review, we focus on social interactions as perhaps the most dynamic and challenging aspects of the lives of farm animals. Social stress is a leading welfare concern in livestock, and substantial variation in social behavior is seen at the individual and group level. Here, we consider how a fundamental understanding of social behavior can be used to: (i) understand agonistic and affiliative interactions in farm animals; (ii) identify how artificial environments influence social behavior and impact welfare; and (iii) provide insights into the mechanisms and development of social behavior. We conclude by highlighting opportunities to build on previous work and suggest potential fundamental hypotheses of applied relevance. Key areas for further research could include identifying the welfare benefits of socio–positive interactions, the potential impacts of disrupting important social bonds, and the role of skill in allowing farm animals to navigate competitive and positive social interactions. Such studies should provide insights to improve the welfare of farm animals, while also being applicable to other contexts, such as zoos and laboratories.
The Omicron (B.1.1.529) SARS-CoV-2 variant of concern also affects companion animals
Authors: Sánchez-Morales Lidia , Sánchez-Vizcaíno José M. , Pérez-Sancho Marta , Domínguez Lucas , Barroso-Arévalo Sandra
Published: August 2022

The emergence of the Omicron variant (B.1. 1.529) has brought with it an increase in the incidence of SARS-CoV-2 disease. However, there is hardly any data on its incidence in companion animals. We have detected the presence of this new variant in domestic animals (dogs and cats) living with infected owners in Spain. None of the RT-qPCR positive animals (10.13%) presented any clinical signs and the viral loads detected were low. In addition, the shedding of viral RNA lasted a short period of time in the positive animals. Infection with this variant of concern (VOC) was confirmed by RT-qPCR and sequencing. These outcomes suggest a lower virulence of this variant in infected cats and dogs. They also demonstrate the transmission from infected humans to domestic animals and highlight the importance of active surveillance as well as genomic research to detect the presence of VOCs or mutations associated with animal hosts.
Analysis of porcine bone marrow-derived macrophage cytokine responses to infection with PRRSV-1 strains of differing virulence
Authors: Jackson Ben , Chrun Tiphany , Childs Kay , Wanasen Nanchaya , Frossard Jean-Pierre , Graham Simon P. , Seago Julian
Published: August 2022

Porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) causes huge economic losses to the European pig industry. PRRSV-1 is divided into 3 subtypes and exhibits considerable antigenic heterogeneity. Due to its high mutation rate, PRRSV-1 is constantly evolving, and highly virulent, particularly subtype 3 strains, are continually emerging. The mechanism(s) underlying PRRSV-1 virulence have not been fully elucidated.In vivostudies have implicated replication kinetics, cell tropism and an enhanced pro-inflammatory cytokine response as potential contributing factors. However, few strains have been directly compared and differences inin vivostudy design have hindered comparison, thus limiting our understanding of PRRSV-1 virulence. To address this knowledge gap, we sought to develop a reverse genetics andex vivomodel system, to attempt to identify correlates of PRRSV-1 virulence and attenuationin vitro. Herein we describe the use of primary porcine bone marrow-derived macrophages (BMDM) to investigate the growth kinetics and induced cytokine profiles of the highly virulent SU1-Bel strain, the low virulence 215-06 strain and the attenuated Olot/91 strain. We show that infection of BMDM with virulent PRRSV-1 strains induced higher expression of IL-6 and IL-8 and lower expression of TNF-α when compared with the attenuated strain. In addition, BMDM infected with SU1-Bel secreted significantly more IFN-α than those infected with PRRSV-1 strains of lower virulence. Interestingly, despite inducing less IFN-α than SU1-Bel, Olot/91 induced much higher levels of expression of several interferon-stimulated genes (ISGs), suggesting that Olot/91 may be less able to counteract type I IFN signaling which may contribute to its attenuated phenotype.
Performance of Forage Cactus Intercropped with Arboreal Legumes and Fertilized with Different Manure Sources
Authors: Saraiva Felipe Martins , Dubeux José Carlos Batista , Cunha Márcio Vieira da , Menezes Rômulo Simões Cezar , dos Santos Mércia Virginia Ferreira , Camelo Dayanne , Ferraz Ivan
Published: August 2022

The use of agricultural practices such as fertilization and intercropping can improve the production of forage cacti. The objective of this study was to evaluate the agronomic characteristics of forage cactus intercropped with leguminous trees and fertilized with different sources of manure in the tropical semiarid region of Brazil. The research was carried out at the Agricultural and Livestock Research Enterprise of Pernambuco State during the period from March 2011 to September 2013. The following cultivation systems were used: (i) Gliricidia sepium (Jacq.) Kunth + forage cactus cv. IPA-Sertania [Opuntia cochenillifera (L.) Mill]; (ii) Leucaena leucocephala (Lam.) de Wit + forage cactus; and (iii) forage cactus in monoculture. All of these systems were fertilized with different sources of manure (cattle, goat, sheep, and broiler litter). The goat and sheep manure (16.6 and 16.5 Mg DM ha−1 year−1) provided the least production of cactus in the different cropping systems. Cattle manure provided greater production of forage and wood from legumes (1.4 and 4.3 Mg DM ha−1 year−1) and cactus (20.9 Mg DM ha−1 year−1). Gliricidia produced more forage and wood than Leucaena. Total production of the forage cactus-Gliricidia system produced 4.7 and 3.8 Mg DM ha−1 of biomass and wood in two years, respectively. The production and morphological characteristics of the cactus increased at further distance from the trees (3 m), and the opposite effect was observed for the concentrations of N, p, and K. Thus, cropping systems using forage cactus and tree legumes fertilized with manure represent an option for tropical semiarid regions.
Soil Available Nitrogen and Yield Effect under Different Combinations of Urease/Nitrate Inhibitor in Wheat/Maize Rotation System
Authors: Cui Xiumin , Wang Jingquan , Wang Jiahui , Li Yun , Lou Yanhong , Zhuge Yuping , Dong Yuxiu
Published: August 2022

In a wheat/maize rotation system, nitrogen (N) accounts for a large proportion of basal fertilizer, but soil N loss and the resulting environmental risk simultaneously exist worldwide. This study applied different urease/nitrification inhibitors together with basal fertilizers and investigated their effects on soil N level and grain yield. Six N stabilizing combinations consisted of two urease inhibitors (HQ and NBPT) and three nitrification inhibitors (DCD, DMPP, and Nitrapyrin). The treatments supplied with urease/nitrification inhibitors reduced, to some degree, the conversion rate of NH4+ into NO3−, and kept NH4+ content higher in surface soils for a longer time. Compared to CK, A1 treatment supplied with 1.5% HQ + 4% DCD well-maintained the levels of soil alkali-hydrolyzable N and NH4+. For example, alkali-hydrolyzable N and NH4+ contents at 0–20 cm soil layer under A1 were increased by 8.59–41.6% and 8.15–14.5% more than CK, respectively. Based on the entire growth period of wheat and maize rotation, urease/nitrification inhibitors improved soil available N in surface soils but did not prevent NH4+ and NO3− leaching, especially in the intensive rainfall season. The combinations of HQ and DCD or Nitrapyrin significantly enhanced crop yield. Specifically, crop yields under A1 and A3 (1.5% HQ + 0.25% Nitrapyrin) were 16.3% and 14.3% higher than CK, respectively. The N stabilizing combinations also promoted N intake and transport at every growth stage. The maximum N accumulation was increased by 27% under A1, when compared to CK. The treatments supplied with urease/nitrification inhibitors also achieved higher apparent N recovery efficiency, N agronomic efficiency, and N partial factor productivity. Consequently, the combinations of urease/nitrification inhibitors could improve N availability at 0–40 cm soil layer, which in turn improved N use efficiency of wheat and maize. The results suggested that the two urease/nitrification inhibitor combinations, 1.5% HQ + 4% DCD (A1) and 1.5% HQ + 0.25% Nitrapyrin (A3), were optimal N stabilizing agents and worthy of further study.