bluuebunny/crossref_metadata_2025 huggingface dataset:
Village Institutes of Turkey
Authors: Meşeci Giorgetti Filiz
Published: December 2021

In the 1930s, the primary schooling rate in Turkey was significantly low compared to the European states. Ninety percent of the population lived in villages without any schools and teachers. Therefore, promoting primary education was addressed as an issue concerning villages in Turkey. The seeds of the intellectual infrastructure in the emergence of institutes were sown at the beginning of the 20th century, during the Ottoman rule. To train teachers for villages, Village Teacher Training School [Köy Muallim Mektebi] was founded in 1927 and Village Instructor Training Course [Köy Eğitmen Kursu] in 1936. However, these initiatives were not sufficient in terms of quality and quantity. Village teacher training experiences, new education, and work school trends of Europe were analyzed by Turkish educators, opinions of foreign and Turkish experts were received, and the Village Institutes [Köy Enstitüleri] project was carried into effect based on the realities of Turkey. The first Village Institutes opened in 1937. They were established in a restricted area, with a limited budget, and a non-common curriculum until the Village Institute Law was promulgated in 1940. On April 17, 1940, the law prescribing their establishment was approved by the parliament. The number of the Village Institutes, which spread over the Turkish geography evenly, reached 21 by 1949. The period between 1940 and 1947 was when the Village Institutes were most productive. Learning by doing and principles of productive work were embraced at the Village Institutes. The curriculum consisted of three components: general culture, agriculture, and technical courses. In addition to their teaching duties, the primary school teachers that graduated from the Village Institutes undertook the mission of guiding villagers in agricultural and technical issues and having them adopt the nation-state ideology in villages. World balances changing after the Second World War also affected the Village Institutes. In 1946, the founding committee of the Village Institutes were accused of leftism and had to leave their offices for political reasons. After the founding committee stepped aside, the Village Institutes started to be criticized by being subjected to the conflict between left-wing and right-wing. Following the government changeover in 1950, radical changes regarding the curricula, students, and teachers of the institutes were made. Making the Village Institutes unique, the production- and work-oriented aspects were eliminated, and the institutes were closed down in 1954 and converted into Primary School Teacher Training Schools. Although the Village Institutes existed only between 1937 and 1954, their social, economic, and political effects were felt for a long time through the teachers, health officers, and inspectors they trained.
Elite School Education Group Policy and Low-Performing Schools in China
Authors: Zhang Yu , Qi Xuan
Published: December 2021

Education inequality has been a challenging issue worldwide, and disparity across schools constitutes a significant proportion of total inequality. Effective policies to turn around low-performing schools (LPS) are therefore of great importance to both governments and students. The Elite School Education Group (ESEG) policy is an emerging one, and it has quickly become very influential in China, a country with one of the largest and most diversified education systems in the world. Under this policy, elite public schools (EPS), which have exceptionally enriched educational resources (i.e., high-quality teachers, strong principal leadership, excellent school cultures, etc.), are encouraged by the government to build school groups with LPS. Within the school group under the elite school brand, branch schools (i.e., the previous LPS) can share all kinds of resources from the EPS (including teachers and principals), and they may even utilize the prestige of the brand itself as a means to attract high-performing students. The ESEG policy enables the delivery of multiple turnaround interventions to LPS in an autonomous way, through building partnerships between EPS and LPS. While some LPS are successfully turned around, some are not. It depends on the effectiveness of the reforms undertaken in the branch schools. Of particular importance is the access to strong principal leadership, excellent teachers, and the school cultures from EPS. Incentives for EPS to participate in this reform include obtaining flexibility in personnel management, expanding school scale and influence, and mobilizing other resources. Despite the potential positive influence on the branch schools, the ESEG policy may have a more complex influence on the entire education ecology than initially expected. Indeed, there are now some concerns that the ESEG is creating new LPS, because more and more high-performing students are drawn out of normal schools and attracted to the ESEG-partnered schools during admission. Thus, the effectiveness of the ESEG policy should not be solely based on attracting high-performing students, but on improving overall education quality.
Constructivism in Education
Authors: Tan Charlene , Ng Connie S.L.
Published: December 2021

In light of the broad, multidimensional, and contestable nature of constructivism, a central debate concerns the object of construction. What do we mean when we say that a learner is constructing something? Three general categories, with overlaps in between, are: the construction of meaning, the construction of knowledge, and the construction of knowledge claims. To construct meaning is to make sense of something by understanding both its parts and overall message. To construct knowledge is to obtain what philosophers traditionally call “justified true belief.” There are three conditions in this formulation of knowledge: belief, truth, and justification. Beliefs are intentional, meaningful, and representational, directing a person to attain truth and avoid error with respect to the very thing that person accepts. As for the notions of truth and justification, there are three major theories of truth, namely the correspondence theory, coherence theory, and pragmatic theory; and seven main types of justification, namely perception, reason, memory, testimony, faith, introspection, and intuition. Finally, to construct a knowledge claim is to indicate that one thinks that one knows something. The crucial difference between knowledge and a knowledge claim is that the latter has not acquired the status of knowledge. There are two main implications for teaching and learning that arise from an epistemological exploration of the concept of constructivism: First, educators need to be clear about what they want their students to construct, and how the latter should go about doing it. Informed by learner profiles and other contingent factors, educators should encourage their students to construct meanings, knowledge, and knowledge claims, individually and collaboratively, throughout their schooling years. Second, educators need to guard against some common misconceptions on constructivism in the schooling context. Constructivism, contrary to popular belief, is compatible with direct instruction, teacher guidance, structured learning, content learning, traditional assessment, and standardized testing. In sum, there are no pedagogical approaches and assessment modes that are necessarily constructivist or anticonstructivist. A variety of teaching methods, resources, and learning environments should therefore be employed to support students in their constructing process.
Umkhonto we Sizwe (MK): The ANC’s Armed Wing, 1961–1993
Authors: Lissoni Arianna
Published: December 2021

Launched in 1961 by leaders of the African National Congress (ANC) of South Africa and the South African Communist Party (SACP), Umkhonto we Sizwe (MK) was the military wing of the ANC until its disbandment in 1993. The initial stage of MK’s armed struggle involved sabotage against government installations and other symbols of the apartheid regime by a small group of operatives. Under increasing repression by the apartheid state, and thanks to the support received from African and socialist countries, MK adopted a strategy of guerrilla warfare as armed struggle assumed an increasingly central role in the liberation struggle, although the military was understood as an extension of political work, that is, linked to the reinvigoration of political struggle and organizations. Geopolitical constraints prevented MK from waging a conventional guerrilla war, and from the 1970s MK adjusted its strategy by turning to armed propaganda and people’s war. While debates on the role of MK in South Africa’s liberation are often reduced to the relative success or failure of military strategy and action, the history of MK remains a sensitive topic post-apartheid, carrying significant weight both symbolically and in the lives of thousands of people who served in its ranks, including women, who joined and participated in MK throughout the three decades of its existence.
Urban Studies of Accra
Authors: Grant Richard
Published: December 2021

Accra is one of the largest and most important cities in sub-Saharan Africa. The aim of this article is to assess the evolution of urban studies in Accra and its main historical and contemporary foci. Early knowledge on urban Accra is fragmentary and orientated toward European contact points and urban plans, ostensibly from the gaze of Europeans. Writings from Euro-Africans such as Carl Reindorf provide a different prism into the precolonial, indigenous, urban society, whereas most indigenous urban knowledge was situated in the oral tradition at this time. Around independence, officially appointed social anthropologists wrote about an indigenous community in Tema and surveyed the multiethnic Accra environment. From independence in 1957 until the early 1980s, social scientists viewed the urban settlement as an alien, Western intervention. Local scholarship on Accra was sidelined as the academy in a poor, emergent nation became preoccupied with the genesis of nation-state building and the establishment of viable academic departments in national universities, and growing proportions of migrants regarded “home” as somewhere else, that is, ancestral villages.

In the 1970s Accra was inserted into world history and social history, and social scientists began to study residential geographies, but scholarship at the city-scale remained sparse. Engagement with world and social histories and the social sciences demonstrated that history matters, but not in linear and teleological ways. The liberalization era ushered in by structural adjustment policies (SAPs) in 1983 invigorated studies of Accra’s urban impacts and effects. Much of this research was disseminated by international scholars, as Ghanaian scholars had to contend with the negative impacts of SAPs on their own universities and households. Since the turn of the 21st century, scholarship on Accra, and African cities in general, has been increasing. Diverse research questions and a multiplicity of methodologies and frameworks seek to engage Western urban theories and other variants, undertake policy-relevant work, assess ethnic and residential dynamics, contribute to international urban debates, and advance postcolonial and revisionist accounts of urbanism. Viewed at the third decade of the 21st century, scholarship on Accra is of diverse origins, encompassing scholarship from locals, members of the diaspora, and international urbanists, and a promising tilt is local–international collaborations co-producing knowledge.
Said, Nicholas
Authors: Salau Mohammed Bashir
Published: December 2021

The two versions of the autobiography that Nicholas Said published offer insight into 19th-century conditions in five continents as well as insight into life as a child, slave, manservant, and teacher. As a child in the 1830s, Said was enslaved in Borno, marched across the Sahara Desert, and passed from hand to hand in North Africa and the Middle East. After serving as a slave in various societies, Said was freed by a Russian aristocrat in the late 1850s after accompanying the aristocrat in question to various parts of Europe. In the 1850s, Said also traveled as a manservant for a European traveler to South and North America. Ultimately he settled in the United States, where he authored two versions of his autobiography, served as a teacher and soldier, got married, and disappeared from sight. This article compares the two versions of the autobiography that Said published, provides an overview of Said’s life, charts the development of scholarly works on Said, and draws attention to the primary sources related to the study of Said and his autobiography.
The Problem of Harm in International Relations
Authors: Hoseason Alexander
Published: December 2021

Harm as a concept lies at the core of the discipline of International Relations (IR), providing a touchstone for scholars that both motivates and frames scholarly practice. However, its pervasive and varied nature means that it is rarely discussed in explicit terms. Attempts to understand the significance of harm for IR, as a pluralist discipline, can be divided into three key perspectives. First, the problem of harm describes a distinct research program centered on the way that social actors have understood, negotiated, and responded to changing forms of harm. Second, different understandings of harm provide a driver of, and a key point of contestation between, IR’s research programs and subdisciplines in ways that reflect the changing dynamics of scholarly interest and normative concern. Third, harm serves to define IR’s objects of inquiry, pointing toward the need for new theoretical tools and innovation in response to global challenges. Taken together, these perspectives suggest that harm serves as an important normative common ground in a discipline that is often understood as pluralist or divided. This common ground serves as a starting point for understanding how harm may change in response to developments or transformations in the international system.
Lupemban
Authors: Taylor Nicholas
Published: December 2021

The Lupemban is an industry of the Middle Stone Age (MSA) that is found across the Congo Basin and on its plateau margins in central Africa. It takes its name from the site of Lupemba that was discovered in 1944 in the Democratic Republic of Congo (DRC, then the Belgian Congo). The Lupemban’s distinctive toolkit of elongated lanceolate bifaces, core-axes, points, blades, and other small tools coincides with the equatorial forest belt and is suitable for constructing hafted implements, which has led to speculation it was a special and specific prehistoric adaptation to rainforest foraging. Although poorly dated across most of its geographic range, radiometric dates for the Lupemban at Twin Rivers (Zambia) show it is at least ~265 ka years old, placing it among the oldest known expressions of the regional MSA. As such, the Lupemban bears on 21st-century debates about the evolution of complex cognitive abilities and behaviors that characterize the emergence of Homo sapiens at or before 300 ka bp. In spite of the Lupemban’s potential importance for understanding the evolution of technology, human–environment interactions, and cognition in early Homo sapiens, the industry remains enigmatic and poorly understood. Logistical, ecological, and political challenges continue to impede fieldwork in central Africa. Moreover, at sites including Gombe Point (DRC), severe soil bioturbation by tree roots has caused the vertical displacement of buried artifacts, which corrupts the basic integrity of stratigraphic sequences. This problem is known to be widespread and means that after 100 years of research, central Africa still lacks a refined Stone Age cultural sequence. Consequently, very little is known about spatiotemporal variability within the Lupemban, or its specific environmental or cultural adaptations. At the site of Kalambo Falls (Zambia), the industry is found in secondary but stratified context, which, as of the early 21st century, offers the best glimpse into Lupemban technology and its potential evolutionary significance.
Death and Dying in the Working Class
Authors: Rosenow Michael K.
Published: December 2021

In the broader field of thanatology, scholars investigate rituals of dying, attitudes toward death, evolving trajectories of life expectancy, and more. Applying a lens of social class means studying similar themes but focusing on the men, women, and children who worked for wages in the United States. Working people were more likely to die from workplace accidents, occupational diseases, or episodes of work-related violence. In most periods of American history, it was more dangerous to be a wage worker than it was to be a soldier. Battlegrounds were not just the shop floor but also the terrain of labor relations. American labor history has been filled with violent encounters between workers asserting their views of economic justice and employers defending their private property rights. These clashes frequently turned deadly. Labor unions and working-class communities extended an ethos of mutualism and solidarity from the union halls and picket lines to memorial services and gravesites. They lauded martyrs to movements for human dignity and erected monuments to honor the fallen. Aspects of ethnicity, race, and gender added layers of meaning that intersected with and refracted through individuals’ economic positions. Workers’ encounters with death and the way they made sense of loss and sacrifice in some ways overlapped with Americans from other social classes in terms of religious custom, ritual practice, and material consumption. Their experiences were not entirely unique but diverged in significant ways.
Civil War Regiments
Authors: Gordon Lesley J.
Published: December 2021

The regiment was the essential “building block” of Civil War armies. Assigned by states, most volunteer regiments were organized based on soldiers’ home residence and reflective of those local communities. Each branch of the army—infantry, artillery, and cavalry—formed into regiments with varying numbers of companies and overall strength. There were regular army regiments and units specially designated for African American troops. As the war dragged on, regimental strengths diminished dramatically. The Confederate Army tried to refill older units with conscripts and new recruits, while the Union created new regiments to replace depleted ones and later consolidated smaller ones. Neither side was entirely successful in restoring regiments to full authorized strength. Nonetheless, the regiment was more than a mode of organization—it was the prime source of identity and pride for volunteers and later veterans. While armies, divisions, and brigades were crucial to winning battles, and companies forged tight bonds of loyalty, it was the regiment to which most soldiers claimed a personal allegiance. Famed regiments like the 1st Minnesota Infantry Regiment, the 1st Texas Infantry Regiment, and the 54th Massachusetts Infantry Regiment cited their battle honors and high casualty numbers as proof of their fighting prowess. After the war ended, veterans produced hundreds of regimental histories, recounting their battle service and seeking to claim a place in history. Although many historians dismiss these accounts as worthless for serious scholarly research, regimental histories offer rich firsthand accounts of the conflict. They also offer a vehicle for narrating the war in a form well familiar to the soldiers who experienced it.
Violeta Parra: Her Life, Work, and Legacy
Authors: Verba Ericka
Published: December 2021

Violeta Parra (1917–1967) was a multifaceted and talented musician and artist. A prolific songwriter, she composed more than two hundred songs as well as experimental pieces for guitar, documentary soundtracks, and music for ballet. Her most famous song, “Gracias a la vida,” has been performed by musicians the world over. In the realm of the visual arts, she was a ceramicist, sculptress, painter, and tapestry maker. In 1964, she became the first Latin American artist to have a solo exhibition at the Louvre Palace’s Musée des Arts Décoratifs. Parra was also an award-winning folklorist who collected hundreds of songs and other folklore from every region of Chile.

Born in southern Chile, she moved to Santiago at age fifteen, where she spent two decades performing a mixture of popular songs from Latin America that is often referred to as música criolla. At age thirty-five she turned to the authentic, first as a folklorist and then as an artist. She was a leader of the Chilean folk revival of the 1950s and inspired the generation of Chilean musicians who formed the protest song movement known as nueva canción in the 1960s. A communist sympathizer, she traveled to Europe as a member of the Chilean delegation to the Soviet-sponsored World Festival of Youth and Students in 1955 (Warsaw) and 1962 (Helsinki). Each time she toured the Soviet Bloc, then made her way to Paris for an extended sojourn.

Parra contributed a significant voice to the national debate over chilenidad (Chilean identity) during a critical juncture in Chile’s economic, social, and cultural development. Her biography sheds light on transnational cultural movements and competing notions of authenticity at the height of the Cold War. It is also the deeply human story of Parra’s tenacious struggle to be seen and heard as an artist on her own terms.
The Amistad Saga: A Transatlantic Dialogue
Authors: Felipe-Gonzalez Jorge , Cole Gibril R. , Lawrance Benjamin N.
Published: December 2021

The story of the slave ship La Amistad is one of the most celebrated and narrated 19th-century stories of the transatlantic slave trade. To fully appreciate the significance and impact of the events and circumstances of this fateful episode, it is important to examine its legacy from multiple points of the Atlantic world—vestiges of the triangular trade bequeathed by the Columbian Exchange. For a long time, the Amistad saga has been viewed from a very US-centric perspective because the dispute over the lives of the Africans rose to the US Supreme Court in 1840–1841. New archival and oral research in West Africa, Europe, and the Caribbean is rebalancing the narrative and revising the historical drama. Today, the Amistad story is widely recognized as a quintessentially Atlantic story, a story of mobility that moves back and forth across the Atlantic in multiple directions over many decades. The deployment of the phrase “Amistad saga” provides a vehicle with which to critique the socio-legal battles about transatlantic slave trading in Caribbean, North American, and West African history. The Amistad story is often described as pre-incidental to the US Civil War. The victory of African defendants is often framed as a self-congratulatory vindication of the successful resistance of enslaved Africans. The celebrated figure of “Joseph Cinqué” or Sengbe Pieh, the self-appointed leader of the Africans, and a replica of the ship itself are part of an Amistad memory industry that attempts to narrate the slave trade and its abolition. A new framework for teaching and understanding the history of the Amistad saga and its memory and forgetting through an Atlantic lens must combine historical and contemporary perspectives from the United States, Europe, Cuba, and Sierra Leone.
Heterogeneous genetic structure in eastern North American peat mosses (Sphagnum)
Authors: Duffy Aaron M , Ricca Mariana , Robinson Sean , Aguero Blanka , Johnson Matthew G , Stenøien Hans K , Flatberg Kjell Ivar , Hassel Kristian , Shaw A Jonathan
Published: December 2021

Bryophytes generally have broad geographical ranges that suggest high dispersal ability. The aim of this study was to test hypotheses about dispersal limitation, as indicated by isolation by distance, in four spore-producing species of the moss genus Sphagnum (Sphagnum carolinianum, Sphagnum missouricum, Sphagnum macrophyllum and Sphagnum pylaesii) and to assess whether plants in the southern USA harbour high levels of unique alleles and/or other indicators of exceptional genetic diversity. Isolation by distance was detected in all four species, but regional patterns of genetic structure were very species specific. Northern and southern genotype groups were detected in S. carolinianum and S. missouricum, but in S. pylaesii plants from the Adirondack Mountains of New York were genetically distinct from others to the north and south. One species, S. macrophyllum, exhibited differentiation between northern and southern genetic groups that appeared to reflect more ancient phylogenetic diversification.
Beyond the call of duty: A Qualitative study into the experiences of family members acting as a Nearest Relative in Mental Health Act assessments
Authors: Dixon Jeremy , Stone Kevin , Laing Judy
Published: December 2021

Research shows that tensions between family carers and professionals become acute where the issue of compulsory admission to hospital is at stake. In England and Wales, a specific family member is appointed to safeguard the interests of a person assessed under the Mental Health Act 1983. This currently occurs through the Nearest Relative (NR) role. The Government is proposing to replace this with a Nominated Person role, chosen by the service user. Drawing on the concept of carer burden, this study reports on the views of nineteen NRs in England to discover their experiences of being involved in a Mental Health Act assessment. Participants identified that they undertook the role due to a sense of duty. Their experiences were mixed with participants highlighting both feelings of distress during the assessment and feelings of relief once their relative had been detained. Participants reported feeling conflicted when their relative was detained and feelings of frustration towards mental health services. The findings have implications for proposals to reform the Mental Health Act 1983. They show that education and support programmes should be created for NRs/Nominated Persons and that research is needed to assess whether such support is effective at reducing carer burden.
Multi-criteria prioritization of the renewable power plants in Australia using the fuzzy logic in decision-making method (FMCDM)
Authors: Aryanfar Amin , Gholami Aslan , Ghorbannezhad Payam , Yeganeh Bijan , Pourgholi Mahdi , Zandi Majid , Stevanovic Svetlana
Published: December 2021

The presented study focused on developing an innovative decision-making framework to select the best renewable-power-plant technologies, considering comprehensive techno-economic and environmental variables. Due to the favourable conditions, Australia was selected as the case study. A fuzzy-logic method and analytical hierarchy process were applied to prioritize different renewable-energy power plants. The techno-economic factors included levelized cost of energy, initial cost, simple payback time, and operation and maintenance costs along with environmental factors including carbon payback time, energy payback time and greenhouse-gas emissions were used to rank the power plants. The results showed that the capital cost and simple payback time had the highest priority from an economic point of view. In comparison, greenhouse-gas emissions and carbon payback time were the dominant environmental factors. The analysis results provided economic and environmental priority tables for developing different power plants in the current state and a future scenario by 2030. The fuzzy results and pairwise composite matrix of alternatives indicated that the onshore wind, offshore wind, single-axis tracker polycrystalline photovoltaic, single-axis tracker monocrystalline photovoltaic, fix-tilted polycrystalline photovoltaic and fix-tilted monocrystalline photovoltaic scored the highest in the current state. In contrast, by 2030, the single-axis tracker photovoltaic power plants will be the best choice in the future scenario in Australia. Finally, the results were used and analysed to recommend and suggest several policy implementations and future research studies.
A maximum power point tracking strategy applied to building integrated photovoltaics
Authors: Cui Jian , Fang Jianjun
Published: December 2021

To solve the problem of permanent-shadow shading of photovoltaic buildings, a maximum power point tracking (MPPT) strategy to determine the search range by pre-delimiting area is proposed to improve MPPT efficiency. The single correspondence between the solar-cell current–voltage (I–V) curve and the illumination conditions was proved by using the single-diode model of photovoltaic cells, thus proving that a change in the illumination conditions corresponds to a unique maximum power point (MPP) search area. According to the approximate relationship between MPP voltage, current and open-circuit voltage and short-circuit current of a photovoltaic module, the voltage region where the MPP is located is determined and the global maximum power point is determined using the power operating triangle strategy in this region. Simulation carried out in MATLAB proves the correctness and feasibility of the theoretical research. Simulation results show that the MPPT strategy proposed in this paper can improve the average efficiency by 1.125% when applied in series as building integrated photovoltaics.
Instantaneous neural processing of communicative functions conveyed by speech prosody
Authors: Tomasello Rosario , Grisoni Luigi , Boux Isabella , Sammler Daniela , Pulvermüller Friedemann
Published: December 2021

During conversations, speech prosody provides important clues about the speaker’s communicative intentions. In many languages, a rising vocal pitch at the end of a sentence typically expresses a question function, whereas a falling pitch suggests a statement. Here, the neurophysiological basis of intonation and speech act understanding were investigated with high-density electroencephalography (EEG) to determine whether prosodic features are reflected at the neurophysiological level. Already approximately 100 ms after the sentence-final word differing in prosody, questions, and statements expressed with the same sentences led to different neurophysiological activity recorded in the event-related potential. Interestingly, low-pass filtered sentences and acoustically matched nonvocal musical signals failed to show any neurophysiological dissociations, thus suggesting that the physical intonation alone cannot explain this modulation. Our results show rapid neurophysiological indexes of prosodic communicative information processing that emerge only when pragmatic and lexico-semantic information are fully expressed. The early enhancement of question-related activity compared with statements was due to sources in the articulatory-motor region, which may reflect the richer action knowledge immanent to questions, namely the expectation of the partner action of answering the question. The present findings demonstrate a neurophysiological correlate of prosodic communicative information processing, which enables humans to rapidly detect and understand speaker intentions in linguistic interactions.
Development and Validation of a Stability-Indicating Reversed-Phase HPLC Method for Assay and Estimation of Related Substances of Ivermectin in an Oral Paste
Authors: Padivitage Nilusha , Tian Jingzhi , Wang Lin , Zhuang Jinyou , McAdoo Andrew , Zhao Daoli , Rustum Abu M
Published: December 2021

Ivermectin is a potent semi-synthetic antiparasitic drug used in veterinary medicine. A reversed-phase high performance liquid chromatography (HPLC) method has been developed and validated for the identification and assay of Ivermectin, including the identification and estimation of its related impurities in an oral paste. Analytes were separated using a gradient elution at a flow rate of 1.5 mL/min on a Zorbax Extend-C18 column (150 mm × 4.6 mm i.d., 3.5-μm particle size) maintained at 30 °C. The mobile phase was composed of water as mobile-phase A and acetonitrile/methanol (85/15, v/v) as mobile-phase B. Ultraviolet detection at 245 nm was employed to monitor the analytes. Limit of quantitation (LOQ) and limit of detection (LOD) of the method are 0.6 and 0.2 μg/mL, respectively. The validation results demonstrated excellent linearity of the method in the range of 0.1–150% of the analytical concentration (0.6 mg/mL) of the method. The stability-indicating capability of the method has been demonstrated by adequately separating the degradation products from the stress degraded samples of the oral paste as per method validation requirements prescribed in the current International Council for Harmonisation guidelines.
The unmet need of evidence-based therapy for patients with advanced chronic kidney disease and heart failure
Authors: Ortiz Alberto , Navarro-González Juan F , Núñez Julio , de la Espriella Rafael , Cobo Marta , Santamaría Rafael , de Sequera Patricia , Díez Javier
Published: December 2021

Despite the high prevalence of chronic kidney disease (CKD) and its high cardiovascular risk, patients with CKD, especially those with advanced CKD (stages 4–5 and patients on kidney replacement therapy), are excluded from most cardiovascular clinical trials. It is particularly relevant in patients with advanced CKD and heart failure (HF) who have been underrepresented in many pivotal randomized trials that have modified the management of HF. For this reason, there is little or no direct evidence for HF therapies in patients with advanced CKD and treatment is extrapolated from patients without CKD or patients with earlier CKD stages. The major consequence of the lack of direct evidence is the under-prescription of HF drugs to this patient population. As patients with advanced CKD and HF represent probably the highest cardiovascular risk population, the exclusion of these patients from HF trials is a serious deontological fault that must be solved. There is an urgent need to generate evidence on how to treat HF in patients with advanced CKD. This article briefly reviews the management challenges posed by HF in patients with CKD and proposes a road map to address them.
Current recommendations and uncertainties for surgical treatment of infective endocarditis: a comparison of American and European cardiovascular guidelines
Authors: Wang Andrew , Fosbøl Emil L
Published: December 2021

Surgery is an effective therapy in the treatment of left-sided infective endocarditis (IE) in patients for whom antibiotic treatment alone is unlikely to be curative or may be associated with ongoing risk of complications. However, the interplay between indication for surgery, its risk, and timing is complex and there continue to be challenges in defining the effects of surgery on disease-related outcome. Guidelines published by the American College of Cardiology/American Heart Association and the European Society of Cardiology provide recommendations for the use of surgery in IE, but these are limited by a low level of evidence related to predominantly observational studies with inherent selection and survival biases. Evidence to guide the timing of surgery in IE is less robust, and predominantly based on expert consensus. Delays between IE diagnosis and recognition of an IE complication as a surgical indication and transfers to surgical centres also impact surgical timing. This comparison of the two guidelines exposes areas of uncertainty and gaps in current evidence for the use of surgery in IE across different indications, particularly related to its timing and consideration of operative risk.
Improving patient care pathways in otitis externa
Authors: Mather Michael W , Mohammed Hassan , Wilson Janet A
Published: December 2021

Introduction

Referrals for otitis externa (OE) have dramatically increased but the reasons for this remain unclear. We aim to characterize management of patients both pre- and post-referral to identify areas of improvement at the primary–secondary care interface.

Methods

Questionnaire study from consultant-led research clinic specifically set up to prospectively analyse OE referrals at a tertiary referral centre for Otolaryngology.

Results

Sixty-two patients were included; 63% female, median age 57 years. One was excluded (clinically not OE). Most patients had multiple primary care visits before referral (average 4 GP; 2 practice nurse). Sixty per cent had received oral antibiotics (16% multiple classes). Eighteen per cent had never had ear drops. Thirty-nine per cent were not advised to keep ears dry. Twenty-one per cent had dermatitis; 13% contact allergy, 30% systemic allergy, 5% diabetes. Less than 10% had narrow canals. Thirty-six per cent had active discharge but <7% needed a wick. Approximately 75% appear suitable for community aural care clinics.

Conclusions

OE occurs most commonly in female patients, often with associated risk factors. Patients often consult primary care several times prior to referral. Lifestyle advice and ototopical drops are frequently overlooked; instead, often inappropriately treated with oral antibiotics. Most ears were anatomically normal, and community aural care clinics may have a role in more timely and accessible treatment.
A tandem duplication in Drosophila melanogaster shows enhanced expression beyond the gene copy number
Authors: Loehlin David W , Kim Jeremiah Y , Paster Caleigh O
Published: December 2021

Tandem duplicated genes are common features of genomes, but the phenotypic consequences of their origins are not well understood. It is not known whether a simple doubling of gene expression should be expected, or else some other expression outcome. This study describes an experimental framework using engineered deletions to assess any contribution of locally acting cis- and globally acting trans-regulatory factors to expression interactions of particular tandem duplicated genes. Acsx1L (CG6300) and Acsx1R (CG11659) are tandem duplicates of a putative acyl-CoA synthetase gene found in Drosophila melanogaster. Experimental deletions of the duplicated segments were used to investigate whether the presence of 1 tandem duplicated block influences the expression of its neighbor. Acsx1L, the gene in the left block, shows much higher expression than either its duplicate Acsx1R or the single Acsx1 in Drosophila simulans. Acsx1L expression decreases drastically upon deleting the right-hand duplicated block. Crosses among wildtype and deletion strains show that high tandem expression is primarily due to cis-acting interactions between the duplicated blocks. No effect of these genes on cuticular hydrocarbons was detected. Sequence and phylogenetic analysis suggest that the duplication rose to fixation in D. melanogaster and has been subject to extensive gene conversion. Some strains actually carry 3 tandem copies, yet strains with 3 Acsx1s do not have higher expression levels than strains with 2. Surveys of tandem duplicate expression have typically not found the expected 2-fold increase in expression. This study suggests that cis-regulatory interactions between duplicated blocks could be responsible for this trend.
The Impact of Marital Quality as Older Couples Adjust to Dementia Onset
Authors: Huo Meng , Kim Kyungmin , Han Sae Hwang
Published: December 2021

Objectives

A burgeoning literature links being married to better cognitive health, but less attention has been paid to how couples view their marital relationships. Couples do not always concur in their assessments, and such discrepancies affect both partners’ health. We present a dyadic study on whether and how overall and discrepant views of marital quality predicted (a) dementia onset and (b) changes in older adults’ depressive symptoms with spousal dementia.

Methods

A pooled sample of couples aged 50+ (dyad N = 3,936) from the Health and Retirement Study rated positive and negative marital quality at baseline (2006/2008). Each participant reported whether they had been told of having dementia and their depressive symptoms once every other year (2006/2008–2014/2016).

Results

Cox proportional hazards regression revealed that older adults who rated their marriages either more positively or more negatively than their spouses were more likely to develop dementia. We applied multiphase growth curve modeling to older adults whose spouses developed dementia, finding that those in marriages that were more negative overall reported more depressive symptoms but exhibited a smaller increase in these symptoms in response to spousal dementia.

Discussion

This study adds to the literature by showing how discrepant marital assessments shape cognitive aging and offers new insights into identifying older adults with greater dementia risk. Findings also revealed the impact of overall negative marital quality on older adults’ psychological adjustment to spousal dementia, which could inform interventions intended to help couples better cope with early-stage dementia from a relational perspective.
Dementia Family Caregiving in Rural Appalachia: A Sociocultural Model of Care Decisions and Service Use
Authors: Roberto Karen A , Savla Jyoti , McCann Brandy Renee , Blieszner Rosemary , Knight Aubrey L
Published: December 2021

Objectives

Examinations of cultural variations in caregiving experiences and practices tend to focus on racially and ethnically diverse family caregivers. We extended this research by exploring the role of place-based cultural constructs on care decisions of family caregivers for persons living with dementia in rural Appalachia whose experiences and backgrounds dwell outside the lines of dominant culture.

Methods

Guided by our sociocultural conceptual model of service use and a descriptive qualitative study design, we conducted in-depth interviews with 30 caregivers for persons living with dementia to understand how predisposing and enabling factors influence caregiving decisions, including the use of services to care for persons living with dementia.

Results

Three predisposing factors reflecting the culture of rural Appalachian counties in Virginia (reasons for becoming a caregiver, previous caregiving experience, and deference to the person living with dementia) shaped decisions about caregiving responsibilities and service use. Three place-based enabling factors (a personal sense of belonging, knowledge of services, and family support for service use) played an important role in facilitating access to services.

Discussion

Findings revealed subtle variation across caregivers in this study and in comparison, to caregivers’ experiences more generally. We interpret these variations in light of culturally influenced attitudes, family norms, and sources of information about formal services. Place-based cultural experience influenced dementia family caregivers’ behaviors and choices regarding formal service use, suggesting that investigators should pay more attention to cultural influences in future caregiving research.
Vaccine hesitancy and acceptance: an examination of predictive factors in COVID-19 vaccination in Saudi Arabia
Authors: Fadhel Fahmi Hassan
Published: December 2021

Vaccine hesitancy is a global health issue and can be affected by several variables. We explored the predictive factors and causes of vaccine hesitancy among adults in Saudi Arabia. An online survey method with multiple regression analysis was used to identify factors predicting of vaccine hesitancy in 558 adults (46.24% women and 53.76% men). The prevalence of vaccine hesitancy is 20.6%, with higher rates among females, young people and single people. About 70% of the participants believe that vaccine hesitancy is due to concerns about the safety and efficacy of the vaccine, a lack of information about the disease and vaccine or social media. The vaccine acceptance rate is 71.3%; 17.2% are not willing to accept a COVID-19 vaccine and 11.5% are unsure. Males and married people are more accepting of the vaccine. The risk factors that predict vaccine hesitancy include age, gender, belief in conspiracy theories and psychosocial factors. Meanwhile, age, gender, belief in conspiracy theories, concerns about the safety and efficacy of the vaccine and psychosocial factors significantly predict vaccine acceptance. The high rate of vaccine hesitancy could undermine efforts to combat COVID-19. Factors predicting vaccine hesitancy can be used in interventions to address this issue during major epidemics.
Modified use of a 4-branched frozen elephant trunk prosthesis for treatment of a right-sided aortic arch aneurysm with an aberrant left subclavian artery
Authors: Brickwedel Jens , Reichenspurner Hermann , Kölbel Tilo , Detter Christian
Published: December 2021

Right aortic arch aneurysm originating from a Kommerell’s diverticulum associated with an aberrant left subclavian artery is a rare congenital entity. We report a case of an asymptomatic 60-year-old female with right aortic arch aneurysm with an aberrant left subclavian artery, treated with a modified frozen elephant trunk technique using a 4-branched prosthesis, with the perfusion branch as an extra-anatomical bypass to the aberrant left subclavian artery. This case demonstrates short-term safety and efficacy of this technique.
Vaccine Effectiveness Against Influenza Hospitalization and Emergency Department Visits in 2 A(H3N2) Dominant Influenza Seasons Among Children <18 Years Old—New Vaccine Surveillance Network 2016–2017 and 2017–2018
Authors: Kim Sara S , Naioti Eric A , Halasa Natasha B , Stewart Laura S , Williams John V , Michaels Marian G , Selvarangan Rangaraj , Harrison Christopher J , Staat Mary A , Schlaudecker Elizabeth P , Weinberg Geoffrey A , Szilagyi Peter G , Boom Julie A , Sahni Leila C , Englund Janet A , Klein Eileen J , Ogokeh Constance E , Campbell Angela P , Patel Manish M ,   New Vaccine Surveillance Network, Hickey Robert W , Piedra Pedro A , Munoz Flor M , Avadhanula Vasanthi , Schuster Jennifer E , Pahud Barbara A , Weddle Gina , Moffatt Mary E , Albertin Christina , Fregoe Wende , McNeal Monica
Published: December 2021

Studies have shown egg-adaptive mutations in influenza vaccine strains that might have impaired protection against circulating A(H3N2) influenza viruses during the 2016–2017 and 2017–2018 seasons. We used the test-negative design and multivariable models to assess vaccine effectiveness against influenza-associated hospitalization and emergency department visits among children (<18 years old) during the 2016–2017 and 2017–2018 seasons. Effectiveness was 71% (95% confidence interval, 59%–79%), 46% (35%–55%), and 45% (33%–55%) against A(H1N1)pdm09, A(H3N2), and B viruses respectively, across both seasons. During high-severity seasons with concerns for vaccine mismatch, vaccination offered substantial protection against severe influenza outcomes requiring hospitalization or emergency department visits among children.
Immune cell mediated cabozantinib resistance for patients with renal cell carcinoma
Authors: Park Keon Young , Hefti Hunter O , Liu Peng , Lugo-Cintrón Karina M , Kerr Sheena C , Beebe David J
Published: December 2021

Renal cell carcinoma (RCC) is the third most common genitourinary cancer in the USA. Despite recent advances in the treatment for advanced and metastatic clear cell RCC (ccRCC), the 5-year relative survival rate for the distant disease remains at 12%. Cabozantinib, a tyrosine kinase inhibitor (TKI), which is one of the first-line therapies approved to treat advanced ccRCC as a single agent, is now being investigated as a combination therapy with newer immunotherapeutic agents. However, not much is known about how cabozantinib modulates the immune system. Here, we present a high throughput tri-culture model that incorporates cancer cells, endothelial cells, and patient-derived immune cells to study the effect of immune cells from patients with ccRCC on angiogenesis and cabozantinib resistance. We show that circulating immune cells from patients with ccRCC induce cabozantinib resistance via increased secretion of a set of pro-angiogenic factors. Using multivariate partial least square regression modeling, we identified CD4+ T cell subsets that are correlated with cabozantinib resistance and report the changes in the frequency of these populations in ccRCC patients who are undergoing cabozantinib therapy. These findings provide a potential set of biomarkers that should be further investigated in the current TKI-immunotherapy combination clinical trials to improve personalized treatments for patients with ccRCC.