bluuebunny/crossref_metadata_2025 huggingface dataset:
Infectious Diseases and Basal Ganglia Calcifications: A Cross-Sectional Study in Patients with Fahr’s Disease and Systematic Review
Authors: Snijders Birgitta M. G. , Peters Mike J. L. , van den Brink Susanne , van Trijp Marijke J. C. A. , de Jong Pim A. , Vissers Laurens A. T. M. , Verduyn Lunel Frans M. , Emmelot-Vonk Marielle H. , Koek Huiberdina L.
Published: April 2024

Background: It is unclear whether patients with basal ganglia calcifications (BGC) should undergo infectious disease testing as part of their diagnostic work-up. We investigated the occurrence of possibly associated infections in patients with BGC diagnosed with Fahr’s disease or syndrome and consecutively performed a systematic review of published infectious diseases associated with BGC. Methods: In a cross-sectional study, we evaluated infections in non-immunocompromised patients aged ≥ 18 years with BGC in the Netherlands, who were diagnosed with Fahr’s disease or syndrome after an extensive multidisciplinary diagnostic work-up. Pathogens that were assessed included the following: Brucella sp., cytomegalovirus, human herpesvirus type 6/8, human immunodeficiency virus (HIV), Mycobacterium tuberculosis, rubella virus, and Toxoplasma gondii. Next, a systematic review was performed using MEDLINE and Embase (2002–2023). Results: The cross-sectional study included 54 patients (median age 65 years). We did not observe any possible related infections to the BGC in this population. Prior infection with Toxoplasma gondii occurred in 28%, and in 94%, IgG rubella antibodies were present. The positive tests were considered to be incidental findings by the multidisciplinary team since these infections are only associated with BGC when congenitally contracted and all patients presented with adult-onset symptoms. The systematic search yielded 47 articles, including 24 narrative reviews/textbooks and 23 original studies (11 case series, 6 cross-sectional and 4 cohort studies, and 2 systematic reviews). Most studies reported congenital infections associated with BGC (cytomegalovirus, HIV, rubella virus, Zika virus). Only two studies reported acquired pathogens (chronic active Epstein–Barr virus and Mycobacterium tuberculosis). The quality of evidence was low. Conclusions: In our cross-sectional study and systematic review, we found no convincing evidence that acquired infections are causing BGC in adults. Therefore, we argue against routine testing for infections in non-immunocompromised adults with BGC in Western countries.
Traumatic Spinal Injury in Children; Time to Revise Pre-Hospital and Diagnostic Protocols?
Authors: Oude Alink Michelle , Stassen Huub , Spoor Jochem , Renkens Jeroen , Moors Xavier , Dremmen Marjolein , Stolker Robert Jan , van der Marel Caroline
Published: April 2024

Background: Traumatic spinal injury in children is a rare but serious life event. Predicting pediatric patients at risk for spinal injury remains difficult. This study focuses on the cause of the injury and predictors to identify children at risk and appropriate diagnostic procedures. Methods: Retrospective chart review from the Landelijke Trauma Registratie of patients with spinal injury from 2010 to 2021 in a level 1 pediatric trauma center. Results: We included 114 children with spinal injury, 79.8% of whom were aged 12–17 years. In the overall trauma population, the incidence of spinal injury was 10% in children aged 12–17 years, 2.3% in children aged 6–11 years, and 0.4% in children 0–5 years of age. Neurological deficits were present in 27.2% of patients in the emergency department, with permanent deficits in 14.0%. Spinal fractures were present in 91.2% of 12–17-year-olds, 43.8% in 6–11-year-olds, and 71.4% in 0–5-year-olds. ISS was 23 (SD 14) in children with spinal injury compared to 8 (SD 9) for children without spinal injury. Conclusions: In children 0–11 years old, spinal injury is very rare compared to the overall trauma population, and there are more non-osseous injuries. Clinicians should consider MRI as the next step after conventional X-ray to diagnose or exclude spinal injuries in this group. In older children aged 12–17 years, the incidence of spinal injury is much higher, at 10%. Although ISS is higher in children with spinal injury, a low ISS does not exclude spinal injury. If one fracture is found, more fractures in other regions of the spine may be present.
Indirect Exposure to Atrocities and Post-Traumatic Stress Disorder Symptoms among Aid Workers: Hemispheric Lateralization Matters
Authors: Levy Einav , Herzog Daniela , Ryder Chen Hanna , Grunstein Rachel , Gidron Yori
Published: April 2024

Background: Humanitarian aid workers (HAWs) are indirectly exposed to atrocities relating to people of concern (POC). This may result in a risk of secondary traumatization demonstrated by post-traumatic stress symptoms (PTSSs). Previous studies have demonstrated that hemispheric lateralization (HL) moderates the relationship between threat exposure and post-traumatic stress symptoms (PTSSs). Aims: We hypothesized that indirect exposure to atrocities (IETA) would be positively correlated with PTSSs among HAWs with right and not left HL. Method: Fifty-four HAWs from several countries that provided humanitarian support in Greece and Colombia participated in this correlational and cross-sectional observation study. They completed scales relating to IETA, PTSSs were assessed using a brief, valid scale, and HL was measured. Results: IETA was positively and significantly related to PTSSs (r = 0.39, p < 0.005). Considering HL, IETA was unrelated to PTSSs among people with right HL (r = 0.29, p = 0.14), while IETA was related to PTSSs among people with left HL (r = 0.52, p = 0.008). Right HL emerged as a protective factor in the relationship between IETA and PTSS. Conclusions: An assessment of dominant HL can serve as one consideration among others when deploying HAWs in specific locations and roles, vis à vis IETA. Moreover, those found to have a higher risk for PTSSs based on their HL could be monitored more closely to prevent adverse reactions to IETA.
Biological and Mechanical Performance of Dual-Setting Brushite–Silica Gel Cements
Authors: Steinacker Valentin C. , Renner Tobias , Holzmeister Ib , Gubik Sebastian , Müller-Richter Urs , Breitenbücher Niko , Fuchs Andreas , Straub Anton , Scheurer Mario , Kübler Alexander C. , Gbureck Uwe
Published: April 2024

Bone defects resulting from trauma, diseases, or surgical procedures pose significant challenges in the field of oral and maxillofacial surgery. The development of effective bone substitute materials that promote bone healing and regeneration is crucial for successful clinical outcomes. Calcium phosphate cements (CPCs) have emerged as promising candidates for bone replacement due to their biocompatibility, bioactivity, and ability to integrate with host tissues. However, there is a continuous demand for further improvements in the mechanical properties, biodegradability, and bioactivity of these materials. Dual setting of cements is one way to improve the performance of CPCs. Therefore, silicate matrices can be incorporated in these cements. Silicate-based materials have shown great potential in various biomedical applications, including tissue engineering and drug delivery systems. In the context of bone regeneration, silicate matrices offer unique advantages such as improved mechanical stability, controlled release of bioactive ions, and enhanced cellular responses. Comprehensive assessments of both the material properties and biological responses of our samples were conducted. Cytocompatibility was assessed through in vitro testing using osteoblastic (MG-63) and osteoclastic (RAW 264.7) cell lines. Cell activity on the surfaces was quantified, and scanning electron microscopy (SEM) was employed to capture images of the RAW cells. In our study, incorporation of tetraethyl orthosilicate (TEOS) in dual-curing cements significantly enhanced physical properties, attributed to increased crosslinking density and reduced pore size. Higher alkoxysilyl group concentration improved biocompatibility by facilitating greater crosslinking. Additionally, our findings suggest citrate’s potential as an alternative retarder due to its positive interaction with the silicate matrix, offering insights for future dental material research. This paper aims to provide an overview of the importance of silicate matrices as modifiers for calcium phosphate cements, focusing on their impact on the mechanical properties, setting behaviour, and biocompatibility of the resulting composites.
The Effect of Sex and Different Repetition Maximums on Kinematics and Surface Electromyography in the Last Repetition of the Barbell Back Squat
Authors: Hegdahl Gundersen Andreas , Nygaard Falch Hallvard , Bao Fredriksen Andrea , Tillaar Roland van den
Published: April 2024

During the ascent phase of a maximal barbell back squat after an initial acceleration, a deceleration region occurs as the result of different biomechanical factors. This is known as the sticking region. However, whether this region is similar in the last repetition of different repetition maximums and if sex has an impact on biomechanics of this region are not known. Therefore, this study investigated the effect of sex (men/women) and repetition maximum (1-, 3-, 6-, and 10RM) on kinematics and surface electromyography around the sticking region. Twenty-six resistance-trained individuals comprising 13 men (body mass: 82.2 ± 8.7; age: 23.6 ± 1.9; height: 181.1 ± 6.5) and 13 women (body mass: 63.6 ± 6.6; age: 23.9 ± 4.5; height: 166.0 ± 4.5) participated in the study. The main findings were that women, in comparison to men, displayed larger trunk lean and lower hip extension angles in the sticking region, possibly due to different hip/knee extensor strength ratios. Moreover, an inverse relationship was discovered between repetition range and timing from V0 to Vmax2, in which lower repetition ranges (1- and 3RM) were shorter in Vmax2 compared to higher ranges (6- and 10RM). It was concluded that this occurrence is due to more moments of inertia in lower repetition ranges. Our findings suggest that both sex and repetition range might induce different requirements during the squat ascent.
Effects of CurraNZ, a New Zealand Blackcurrant Extract during 1 Hour of Treadmill Running in Female and Male Marathon des Sables Athletes in Hot Conditions: Two Case Studies
Authors: Willems Mark E. T. , Bray Patrick W. , Bassett Holly M. , Spurr Tilly J. , West Andrew T.
Published: April 2024

Four weeks before competition in the 2023 Marathon des Sables, a 6-stage, ~250 km running event in the Sahara Desert, we examined the effects of a 7-day intake of New Zealand blackcurrant extract (210 mg anthocyanins per day) on 1 h treadmill running-induced physiological and metabolic responses in the heat (~34 °C, relative humidity: ~30%) in non-acclimatized amateur female and male athletes (age: 23, 38 yrs, BMI: 24.2, 28.4 kg·m−2, body fat%: 29.2, 18.8%, V˙O2max: 50.1, 52.1 mL·kg−1·min−1). During the 1 h run at 50%V˙O2max (speed female: 7.3, male: 7.5 km·h−1), indirect calorimetry was used, and heart rate was recorded at 15 min intervals with core temperature monitoring (0.05 Hz). The 1 h runs took place 3 h after a light breakfast and 2 h after intake of the final dose of New Zealand blackcurrant extract with water allowed ad libitum during the run. The New Zealand blackcurrant extract had no effects on the female athlete. The respiratory exchange ratio (RER) of the female athlete in the non-supplement control condition was 0.77 ± 0.01, indicating an existing ~77% contribution of fat oxidation to the energy requirements. In the male athlete, during 1 h of running, fat oxidation was higher by 21% (p < 0.01), carbohydrate oxidation was 31% lower (p = 0.05), RER was 0.03 units lower (p = 0.04), and core temperature was 0.4 °C lower (p < 0.01) with no differences for heart rate, minute ventilation, oxygen uptake, and carbon dioxide production for the New Zealand blackcurrant condition compared to the non-supplement control condition. Seven-day intake of New Zealand blackcurrant extract (210 mg anthocyanins per day) provided beneficial physiological and metabolic responses during exertional heat stress by 1 h of indoor (~34 °C) treadmill running in a male Marathon des Sables athlete 4 weeks before competition. Future work is required to address whether New Zealand blackcurrant provides a nutritional ergogenic effect for Marathon des Sables athletes during long-duration running in the heat combined with personalized nutrition.
Effects of Ocean Acidification and Summer Thermal Stress on the Physiology and Growth of the Atlantic Surfclam (Spisula solidissima)
Authors: Steeves Laura , Honecker Molly , Meseck Shannon L. , Munroe Daphne
Published: April 2024

This study examines the physiological response of the Atlantic surfclam (Spisula solidissima) to ocean acidification in warm summer temperatures. Working with ambient seawater, this experiment manipulated pH conditions while maintaining natural diel fluctuations and seasonal shifts in temperature. One-year-old surfclams were exposed to one of three pH conditions (ambient (control): 7.8 ± 0.07, medium: 7.51 ± 0.10, or low: 7.20 ± 0.10) in flow-through conditions for six weeks, and feeding and digestive physiology was measured after one day, two weeks, and six weeks. After six weeks of exposure to medium and low pH treatments, growth was not clearly affected, and, contrastingly, feeding and digestive physiology displayed variable responses to pH over time. Seemingly, low pH reduced feeding and absorption rates compared to both the medium treatment and ambient (control) condition; however, this response was clearer after two weeks compared to one day. At six weeks, suppressed physiological rates across both pH treatments and the ambient condition suggest thermal stress from high ambient water temperatures experienced the week prior (24–26 °C) dominated over any changes from low pH. Results from this study provide important information about reduced energy acquisition in surfclams in acidified environments and highlight the need for conducting multistressor experiments that consider the combined effects of temperature and pH stress.
What Drives Asset Returns Comovements? Some Empirical Evidence from US Dollar and Global Stock Returns (2000–2023)
Authors: Tronzano Marco
Published: April 2024

This paper focuses on returns comovements in global stock portfolios including the US Dollar as a defensive asset. The main contribution is the selection of a large set of macroeconomic and financial variables as potential drivers of these comovements and the emphasis on the predictive accuracy of proposed econometric models. One-year US Expected Inflation stands out as the most important predictor, while models including a larger number of variables yield significant predictive gains. Larger forecast errors, due to parameters instabilities, are documented during major financial crises and the COVID-19 pandemic period. Some research directions to improve the forecasting power of econometric models are discussed in the concluding section.
Full Transfer and Segmental Emergence in the L2 Acquisition of Phonology: A Case Study
Authors: Nulahan Anaer , Rose Yvan
Published: April 2024

In this paper, we discuss a child Kazakh speaker’s acquisition of English as her second language. In particular, we focus on this child’s development of the English segments |f, v, θ, ð, ɹ, ʃ, ʧ|, which are not part of the Kazakh phonological inventory of consonants. We begin with a longitudinal description of the patterns that the child displayed through her acquisition of each of these segments. The data reveal patterns that range from extremely rapid to rather slow and progressive acquisition. The data also reveal patterns that were unexpected at first, for example, the slow development of |ʧ| in syllable onsets, an affricate that occurs as a contextual allophone in syllable onsets in Kazakh. We analyze these patterns through the Phonological Interference hypothesis, which was recently extended into the Feature Redistribution and Recombination hypothesis. These models predict the transfer into the L2 of all of the relevant phonological features present within the learner’s first language and their recombination to represent segments present in the L2. We also discuss contexts where feature-based approaches to L2 acquisition fail to capture the full range of observations. In all such contexts, we show that the facts are modulated by phonetic characteristics of the speech sounds present in either the child’s L1 or her L2.
Robustness and Complexity in Italian Mid Vowel Contrasts
Authors: Renwick Margaret E. L.
Published: April 2024

Accounts of phonological contrast traditionally invoke a binary distinction between unpredictable lexically stored phonemes and contextually predictable allophones, whose patterning reveals speakers’ knowledge about their native language. This paper explores the complexity of contrasts among Italian mid vowels from a multifaceted perspective considering the lexicon, linguistic structure, usage, and regional variety. The Italian mid vowels are marginally contrastive due to a scarcity of minimal pairs alongside variation in phonetic realization. The analysis considers corpus data, which indicate that the marginal contrasts among front vowels vs. back vowels are driven by different sources and forces. Functional loads are low; while front /e ɛ/ have the weakest lexical contrast among all Italian vowels, back /o ɔ/ are separated by somewhat more minimal pairs. Among stressed front vowels, height is predicted by syllable structure and is context-dependent in some Italian varieties. Meanwhile, the height of back mid vowels is predicted by lexical frequency, in line with expectations of phonetic reduction in high-frequency contexts. For both front and back vowels, the phonetic factor of duration predicts vowel height, especially in closed syllables, suggesting its use for contrast enhancement. The results have implications for a proposed formalization of Italian mid vowel variation.
Applied Cardio-Oncology in Hematological Malignancies: A Narrative Review
Authors: Mandala Evdokia , Lafara Kyranna , Kokkinovasilis Dimitrios , Kalafatis Ioannis , Koukoulitsa Vasiliki , Katodritou Eirini , Lafaras Christos
Published: April 2024

Applied cardio-oncology in hematological malignancies refers to the integration of cardiovascular care and management for patients with blood cancer, particularly leukemia, lymphoma, and multiple myeloma. Hematological cancer therapy-related cardiotoxicity deals with the most common cardiovascular complications of conventional chemotherapy, targeted therapy, immunotherapy, chimeric antigen receptor T (CAR-T) cell and tumor-infiltrating lymphocyte therapies, bispecific antibodies, and hematopoietic stem cell transplantation. This narrative review focuses on hematological cancer-therapy-related cardiotoxicity’s definition, risk stratification, multimodality imaging, and use of cardiac biomarkers to detect clinical and/or subclinical myocardial dysfunction and electrical instability. Moreover, the most common cardiotoxic profiles of the main drugs and/or therapeutic interventions in patients with hematological malignancies are described thoroughly.
Are Gamers Prone to eThrombosis during Long Gaming Sessions?
Authors: Krarup Kasper B. , Krarup Henrik B. , Mørk Morten , Lundbye-Christensen Søren , Handberg Aase , Nguyen Hien T. T. , Pedersen Inge S. , Kristensen Søren R.
Published: April 2024

During the last two decades, several cases of venous thrombosis (VTE) after a prolonged period at a computer have been described, denominated as “eThrombosis”. Video gaming on a computer has become very popular and can be a social activity where several players gather to play against each other or in a virtual environment for several days (“LAN (i.e., Local Area Network) parties”) where the participants are sedentary and consuming calorie-rich food items. The aim of this study was to investigate potential coagulation activation during a 42 h LAN party. Nine male gamers volunteered for the LAN party. Citrated blood was sampled before and every 6 h, and plasma was analyzed for thrombin generation, thrombin–antithrombin complexes (TAT), prothrombin fragment 1 + 2 (F1 + 2), and D-dimer. Thrombin generation increased slightly but not significantly during the LAN party, whereas the coagulation activation markers were unchanged. These results do not indicate that the coagulation system is activated significantly during 42 h of gaming with minimal physical activity. Although increased activity cannot be excluded, it does not directly indicate a risk of VTE in general.
Structural Superlubricity of Two-Dimensional Materials: Mechanisms, Properties, Influencing Factors, and Applications
Authors: Wu Fan-Bin , Zhou Sheng-Jian , Ouyang Jia-Hu , Wang Shu-Qi , Chen Lei
Published: April 2024

Structural superlubricity refers to the lubrication state in which the friction between two crystalline surfaces in incommensurate contact is nearly zero; this has become an important branch in recent tribological research. Two-dimensional (2D) materials with structural superlubricity such as graphene, MoS2, h-BN, and alike, which possess unique layered structures and excellent friction behavior, will bring significant advances in the development of high-performance microelectromechanical systems (MEMS), as well as in space exploration, space transportation, precision manufacturing, and high-end equipment. Herein, the review mainly introduces the tribological properties of structural superlubricity among typical 2D layered materials and summarizes in detail the underlying mechanisms responsible for superlubricity on sliding surfaces and the influencing factors including the size and layer effect, elasticity effect, moiré superlattice, edge effect, and other external factors like normal load, velocity, and temperature, etc. Finally, the difficulties in achieving robust superlubricity from micro to macroscale were focused on, and the prospects and suggestions were discussed.
A Comprehensive Survey on Deep Learning Methods in Human Activity Recognition
Authors: Kaseris Michail , Kostavelis Ioannis , Malassiotis Sotiris
Published: April 2024

Human activity recognition (HAR) remains an essential field of research with increasing real-world applications ranging from healthcare to industrial environments. As the volume of publications in this domain continues to grow, staying abreast of the most pertinent and innovative methodologies can be challenging. This survey provides a comprehensive overview of the state-of-the-art methods employed in HAR, embracing both classical machine learning techniques and their recent advancements. We investigate a plethora of approaches that leverage diverse input modalities including, but not limited to, accelerometer data, video sequences, and audio signals. Recognizing the challenge of navigating the vast and ever-growing HAR literature, we introduce a novel methodology that employs large language models to efficiently filter and pinpoint relevant academic papers. This not only reduces manual effort but also ensures the inclusion of the most influential works. We also provide a taxonomy of the examined literature to enable scholars to have rapid and organized access when studying HAR approaches. Through this survey, we aim to inform researchers and practitioners with a holistic understanding of the current HAR landscape, its evolution, and the promising avenues for future exploration.
Multi-Objective Optimization for a Partial Disassembly Line Balancing Problem Considering Profit and Carbon Emission
Authors: Yang Wanlin , Li Zixiang , Zheng Chenyu , Zhang Zikai , Zhang Liping , Tang Qiuhua
Published: April 2024

Disassembly lines are widely utilized to disassemble end-of-life products. Most of the research focuses on the complete disassembly of obsolete products. However, there is a lack of studies on profit and on carbon emission saved. Hence, this study considers the multi-objective partial disassembly line balancing problem with AND/OR precedence relations to optimize profit, saved carbon emission and line balance simultaneously. Firstly, a multi-objective mixed-integer programming model is formulated, which could optimally solve the small number of instances with a single objective. Meanwhile, an improved multi-objective artificial bee colony algorithm is developed to generate a set of high-quality Pareto solutions. This algorithm utilizes two-layer encoding of the task permutation vector and the number of selected parts, and develops two-phase decoding to handle the precedence relation constraint and cycle time constraint. In addition, the modified employed bee phase utilizes the neighborhood operation, and the onlooker phase utilizes the crossover operator to achieve a diverse population. The modified scout phase selects a solution from the Pareto front to replace the abandoned individual to obtain a new high-quality solution. To test the performance of the proposed algorithm, the algorithm is compared with the multi-objective simulated annealing algorithm, the original multi-objective artificial bee colony algorithm and the well-known fast non-dominated genetic algorithm. The comparative study demonstrates that the proposed improvements enhance the performance of the method presented, and the proposed methodology outperforms all the compared algorithms.
Effects of Small Random Perturbations in the Extended Glass–Kauffman Model of Gene Regulatory Networks
Authors: Ponosov Arcady , Shlykova Irina , Kadiev Ramazan I.
Published: April 2024

A mathematical justification of some basic structural properties of stochastically perturbed gene regulatory networks, including those with autoregulation and delay, is offered in this paper. By using the theory of stochastic differential equations, it is, in particular, shown how to control the asymptotic behavior of the diffusion terms in order to not destroy certain qualitative features of the networks, for instance, their sliding modes. The results also confirm that the level of randomness is gradually reduced if the gene activation times become much smaller than the time of interaction of genes. Finally, the suggested analysis explains why the deterministic numerical schemes based on replacing smooth, steep response functions by the simpler yet discontinuous Heaviside function, the well-known simplification algorithm, are robust with respect to uncertainties in data. The main technical difficulties of the analysis are handled by applying the uniform version of the stochastic Tikhonov theorem in singular perturbation analysis suggested by Yu. Kabanov and S. Pergamentshchikov.
Cracking the Code: Investigating the Correlation between Aerobic Vaginitis and Preterm Labor
Authors: Zarmakoupi Panagiota , Psarris Alexandros , Karasmani Christina , Antsaklis Panagiotis , Theodora Marianna , Syndos Michael , Pampanos Andreas , Pappa Kalliopi I. , Domali Ekaterini , Thomakos Nikolaos , Akinosoglou Karolina , Tsiakalos Aristotelis , Daskalakis George
Published: April 2024

Aerobic vaginitis (AV) is a distinct clinical entity characterized by inflammation and abnormal vaginal microflora. Often mistaken for bacterial vaginosis, AV remains relatively unknown and underdiagnosed. AV’s understanding is evolving, with some experts suggesting it may primarily be an immunological disorder, the prevalence of which has a range of 7–13% in non-pregnant women and 4.1–8.3% during pregnancy. Pregnancy can affect susceptibility to vaginal infections, leading to adverse outcomes for the woman and the newborn. This review summarizes the correlation between AV and adverse pregnancy outcomes, particularly preterm birth, the leading cause of morbidity and mortality among neonates. An improved understanding of AV’s impact on pregnancy outcomes can lead to early recognition, proper management, and effective interventions. While some studies support an association between AV and preterm labor, the existing knowledge of this relationship remains limited. The evidence suggests that AV may contribute to adverse pregnancy outcomes, mainly preterm birth, but further research is needed to establish a definitive link. Further studies are needed to investigate the underlying mechanisms and clarify AV’s role in premature labor. A comprehensive understanding of AV’s impact on pregnancy outcomes is crucial for early recognition, appropriate management, and effective interventions.
Uterine Cesarean Scar Tissue—An Immunohistochemical Study
Authors: Ziętek Maciej , Świątkowska-Feund Małgorzata , Ciećwież Sylwester , Machałowski Tomasz , Szczuko Małgorzata
Published: April 2024

Background and Objectives: Wound healing encompasses a multitude of factors and entails the establishment of interactions among components of the basement membrane. The quantification of particle concentrations can serve as valuable biomarkers for assessing biomechanical muscle properties. The objective of this study was to examine the immunoexpression and immunoconcentration of myometrial collagen type VI, elastin, alpha-smooth muscle actin, and smooth muscle myosin heavy chain, as well as the expression of platelets and clusters of differentiation 31 in the uterine scar following a cesarean section (CS). Materials and Methods: A total of 177 biopsies were procured from a cohort of pregnant women who were healthy, specifically during the surgical procedure of CS. The participants were categorized into seven distinct groups. Group 1 consisted of primiparas, with a total of 52 individuals. The subsequent groups were organized based on the duration of time that had elapsed since their previous CS. The analysis focused on the immunoexpression and immunoconcentration of the particles listed. Results: No significant variations were observed in the myometrial immunoconcentration of collagen type VI, elastin, smooth muscle myosin, and endothelial cell cluster of differentiation 31 among the analyzed groups. The concentration of alpha-smooth muscle actin in the myometrium was found to be significantly higher in patients who underwent CS within a period of less than 2 years since their previous CS, compared to those with a longer interval between procedures. Conclusions: Our findings indicate that the immunoconcentration of uterine myometrial scar collagen type VI, elastin, smooth muscle myosin heavy chain, alpha-smooth muscle actin, and endothelial cell marker cluster of differentiation 31 remains consistent regardless of the duration elapsed since the previous CS. The findings indicate that there are no significant alterations in the biomechanical properties of the uterine muscle beyond a period of 13 months following a CS.
Effects of the Neuropeptides Pituitary Adenylate Cyclase Activating Polypeptide and Vasoactive Intestinal Peptide in Male Fertility
Authors: Bdeir Roba , Al-Keilani Maha S. , Khamaiseh Khaldoun
Published: April 2024

Background and Objectives: The neuroendocrine system plays a crucial role in regulating various bodily functions, including reproduction, with evidence suggesting its significant involvement in male fertility and sperm development. Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase activating polypeptide (PACAP) are expressed in both male and female reproductive tissues, influencing penile erection and regulating steroidogenesis in males. Therefore, our study aimed to compare the protein levels of VIP and PACAP in seminal plasma between healthy controls and sub-fertile patients. Additionally, we sought to correlate the levels of these biomarkers with clinical, functional, and laboratory findings in the participants. Materials and Methods: The study included a total of 163 male participants for analysis. The participants were further stratified into subgroups of fertile and sub-fertile men of four subgroups according to the 2021 WHO guidelines. Seminal plasma concentrations of the neuropeptides VIP and PACAP were measured using human enzyme-linked immunosorbent assay technique. Results: The findings showed statistically significant differences in total sperm count, sperm concentration, total motility, and vitality (p < 0.001) between the fertile group and the sub-fertile group. Specifically, significant differences found between healthy males and oligoasthenospermic patients (p = 0.002), and between asthenospermic and oligoasthenospermic patients (p = 0.039). An ROC analysis showed associated sensitivity and specificity values of 62.2% and 55.6%, respectively, to PACAP seminal levels differentiated between sub-fertile patients from fertile males (p = 0.028). No significant difference in seminal levels of VIP was found between the sub-fertile and fertile groups. Conclusions: Previous research leads to the point of PACAP active involvement in spermatogenesis. In accordance to our study, in human semen samples, we have seen a significance change in PACAP levels amongst patients with low sperm count or with both low sperm count and low motility, hinting at its contribution and acting as a possible factor in this complex process. Thus, alterations in the levels or actions of these neuropeptides have been associated with certain reproductive disorders in males.
Health Effects of Ionizing Radiation on the Human Body
Authors: Talapko Jasminka , Talapko Domagoj , Katalinić Darko , Kotris Ivan , Erić Ivan , Belić Dino , Vasilj Mihaljević Mila , Vasilj Ana , Erić Suzana , Flam Josipa , Bekić Sanja , Matić Suzana , Škrlec Ivana
Published: April 2024

Radioactivity is a process in which the nuclei of unstable atoms spontaneously decay, producing other nuclei and releasing energy in the form of ionizing radiation in the form of alpha (α) and beta (β) particles as well as the emission of gamma (γ) electromagnetic waves. People may be exposed to radiation in various forms, as casualties of nuclear accidents, workers in power plants, or while working and using different radiation sources in medicine and health care. Acute radiation syndrome (ARS) occurs in subjects exposed to a very high dose of radiation in a very short period of time. Each form of radiation has a unique pathophysiological effect. Unfortunately, higher organisms—human beings—in the course of evolution have not acquired receptors for the direct “capture” of radiation energy, which is transferred at the level of DNA, cells, tissues, and organs. Radiation in biological systems depends on the amount of absorbed energy and its spatial distribution, particularly depending on the linear energy transfer (LET). Photon radiation with low LET leads to homogeneous energy deposition in the entire tissue volume. On the other hand, radiation with a high LET produces a fast Bragg peak, which generates a low input dose, whereby the penetration depth into the tissue increases with the radiation energy. The consequences are mutations, apoptosis, the development of cancer, and cell death. The most sensitive cells are those that divide intensively—bone marrow cells, digestive tract cells, reproductive cells, and skin cells. The health care system and the public should raise awareness of the consequences of ionizing radiation. Therefore, our aim is to identify the consequences of ARS taking into account radiation damage to the respiratory system, nervous system, hematopoietic system, gastrointestinal tract, and skin.
Microstructure Evolution and Strengthening Mechanisms of Mg–Steel Welds Subjected to Multiple Microshot Peening Treatment
Authors: Wang Jianghui , Xu Chuan
Published: April 2024

A surface modification through multiple microshot peening (MSP) was performed on Mg–steel weldment. Application of MSP was found beneficial to the elimination of surface microdefects owing to severe plastic deformation induced by MSP. Moreover, MSP treatment transformed the residual tensile stress of the weld surface into residual compressive stress, which was beneficial to inhibit the initiation and propagation of surface microdefects. Strain strengthening and grain refining were introduced into the shot peened joint, resulting in the notable increase in surface hardness and tensile strength. Compared with an untreated joint, the tensile strength of optimized Mg/steel weldment was markedly enhanced and raised 28% to 244 MPa, and fracture ultimately occurred in the Mg alloy base material. Moreover, the refinement of weld grain induced by MSP treatment was beneficial to strengthen the stress corrosion sensitivity of Mg/steel joints, while also promoting the formation of a denser Mg(OH)2 passivation film on the weld surface and enhancing the corrosion resistance of the joints.
First Principles Study of the Effects of Si, P, and S on the ∑5 (210)[001] Grain Boundary of γ-Fe
Authors: Xu Ying , Cao Weigang , Huang Mengzhe , Zhang Fucheng
Published: April 2024

Solutes segregating at the grain boundary (GB) have a significant influence on the mechanical and chemical properties of steel. In this study, the segregation effects of Si, P, and S on γ-Fe ∑5 (210)[001] GB were systematically analyzed with solution energy, segregation energy, and tensile tests by using a first principles calculation. Si, P, and S are preferred to segregate at substitutional sites in the first layer near the GB. The variation in atomic configuration and electron distribution were investigated by the analysis of bond lengths, charge density, charge density difference, and density of states (DOS), which is caused by the atomic size and electronegativity of solute atoms. Through tensile tests, it was found that Si has a strengthening effect on GB, while P and S exhibit embrittlement effects at low concentration. As the concentration of solutes increase, the segregation sites of P are different from the others owing to the tendency to form Fe3P. The exhibited embrittlement effect is mitigated at first and then aggravated. However, in both cases Si and S show aggravating embrittlement effects on GB cohesion, while the effect of Si changes from strengthening to embrittlement. This work provides comprehensive insights into the effects of Si, P, and S, which will be a useful guidance in steel design.
The Structure and Magnetic Properties of Sm2Fe17Cx Compounds Prepared from Ball-Milled Mixtures of Sm2Fe17 and Carbon Nanotubes or Graphite
Authors: Mikheev Vladislav , Bordyuzhin Igor , Gorshenkov Mikhail , Savchenko Elena , Dorofievich Irina , Shchetinin Igor
Published: April 2024

The processing route of Sm2Fe17 carbides is shorter than that of nitrides, which can potentially be used for cost-effective mid-performance magnets’ production. The magnetic properties of Sm2Fe17Cx compounds can be controlled at the annealing step, which allows them to be used for a variety of applications. In this work, X-ray diffraction (XRD) analysis, Mössbauer spectroscopy, scanning and transmission electron microscopy (SEM, TEM) and vibrating sample magnetometry (VSM) were used for characterization of the structure and magnetic properties of Sm2Fe17Cx compounds. The powder samples were prepared by high-energy ball milling of Sm2Fe17 mixtures with carbon nanotubes (CNT) or graphite with subsequent annealing. The formation of Sm2Fe17Cx compounds after annealing was followed by the formation of α-Fe and amorphous Sm2O3. The hyperfine field values of Fe atoms of all the Sm2Fe17 lattice sites increased by 12% on average after annealing that was caused by carbon diffusion. The coercivity of the samples peaked after annealing at 375 °C. The samples with CNT demonstrated an increase of up to 14% in coercivity and 5% in specific remanence in the range of 250–375 °C annealing temperatures.
Microstructure and Properties in Metals and Alloys (Volume 2)
Authors: Di Schino Andrea , Testani Claudio
Published: April 2024

Microstructure design is key in targeting the desired material’s properties [...]
Computational Applications: Beauvericin from a Mycotoxin into a Humanized Drug
Authors: Al Khoury Charbel , Tokajian Sima , Nemer Nabil , Nemer Georges , Rahy Kelven , Thoumi Sergio , Al Samra Lynn , Sinno Aia
Published: April 2024

Drug discovery was initially attributed to coincidence or experimental research. Historically, the traditional approaches were complex, lengthy, and expensive, entailing costly random screening of synthesized compounds or natural products coupled with in vivo validation largely depending on the availability of appropriate animal models. Currently, in silico modeling has become a vital tool for drug discovery and repurposing. Molecular docking and dynamic simulations are being used to find the best match between a ligand and a molecule, an approach that could help predict the biomolecular interactions between the drug and the target host. Beauvericin (BEA) is an emerging mycotoxin produced by the entomopathogenic fungus Beauveria bassiana, being originally studied for its potential use as a pesticide. BEA is now considered a molecule of interest for its possible use in diverse biotechnological applications in the pharmaceutical industry and medicine. In this manuscript, we provide an overview of the repurposing of BEA as a potential therapeutic agent for multiple diseases. Furthermore, considerable emphasis is given to the fundamental role of in silico techniques to (i) further investigate the activity spectrum of BEA, a secondary metabolite, and (ii) elucidate its mode of action.
Epidemiological and Clinical Aspects of Cutaneous and Mucosal Leishmaniases in Portugal: Retrospective Analysis of Cases Diagnosed in Public Hospitals and Reported in the Literature between 2010 and 2020
Authors: Rocha Rafael , Conceição Cláudia , Gonçalves Luzia , Carvalho Ana Cláudia , Maia André , Martins André , Carujo António , Maio António , Forra Catarina , Melita Catarina , Couto Daniela , Fernandes Diana , Pereira Dulce , Leal Ema , Sarmento Helena , Sousa Inês , Gonçalves Jean-Pierre , Marinho Joana , Vasconcelos Joana , Cunha João , Rodrigues João , Silva José Miguel , Caley Lídia , Malheiro Luís , Santos Luís , Garcia Margarida , Cunha Maria , Lima Maria , Andrade Maria Margarida , Marques Marta , Alpalhão Miguel , Silva Mónica , Ferraz Rita , Soares Rui , Fernandes Salomão , Llobet Samuel , Cruz Sofia , Guimarães Teresa , Branco Tiago , Robalo-Nunes Tomás , Almeida Vasco , Maia Carla
Published: April 2024

Leishmania infantum, a zoonotic vector-born parasite, is endemic in the Mediterranean region, presenting mostly as visceral (VL), but also as cutaneous (CL) and mucosal leishmaniasis (ML). This study aimed to describe the epidemiological and clinical aspects of the CL and ML cases diagnosed in mainland Portugal between 2010 and 2020. Collaboration was requested from every hospital of the Portuguese National Health System. Cases were screened through a search of diagnostic discharge codes or positive laboratory results for Leishmania infection. Simultaneously, a comprehensive literature search was performed. Descriptive statistics and hypothesis testing were performed using IBM® SPSS® Statistics. A total of 43 CL and 7 ML cases were identified, with a predominance of autochthonous cases (86%). In CL, immunosuppressed individuals constituted a significant proportion of patients (48%), and in this group, disseminated CL (22%) and simultaneous VL (54%) were common. In autochthonous cases, lesions, mostly papules/nodules (62%), were frequently observed on the head (48%). The approach to treatment was very heterogeneous. ML cases were all autochthonous, were diagnosed primarily in older immunosuppressed individuals, and were generally treated with liposomal amphotericin B. The findings suggest a need for enhanced surveillance and reporting, clinical awareness, and diagnostic capacity of these forms of leishmaniasis to mitigate underdiagnosis and improve patient outcomes. A holistic One Health approach is advocated to address the multifaceted challenges posed by leishmaniases in Portugal and beyond.
Ligand-Free Silver Nanoparticles: An Innovative Strategy against Viruses and Bacteria
Authors: Morone Maria Vittoria , Chianese Annalisa , Dell’Annunziata Federica , Folliero Veronica , Lamparelli Erwin Pavel , Della Porta Giovanna , Zannella Carla , De Filippis Anna , Franci Gianluigi , Galdiero Massimiliano , Morone Antonio
Published: April 2024

The spread of antibiotic-resistant bacteria and the rise of emerging and re-emerging viruses in recent years constitute significant public health problems. Therefore, it is necessary to develop new antimicrobial strategies to overcome these challenges. Herein, we describe an innovative method to synthesize ligand-free silver nanoparticles by Pulsed Laser Ablation in Liquid (PLAL-AgNPs). Thus produced, nanoparticles were characterized by total X-ray fluorescence, zeta potential analysis, transmission electron microscopy (TEM), and nanoparticle tracking analysis (NTA). A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to evaluate the nanoparticles’ cytotoxicity. Their potential was evaluated against the enveloped herpes simplex virus type 1 (HSV-1) and the naked poliovirus type 1 (PV-1) by plaque reduction assays and confirmed by real-time PCR and fluorescence microscopy, showing that nanoparticles interfered with the early stage of infection. Their action was also examined against different bacteria. We observed that the PLAL-AgNPs exerted a strong effect against both methicillin-resistant Staphylococcus aureus (S. aureus MRSA) and Escherichia coli (E. coli) producing extended-spectrum β-lactamase (ESBL). In detail, the PLAL-AgNPs exhibited a bacteriostatic action against S. aureus and a bactericidal activity against E. coli. Finally, we proved that the PLAL-AgNPs were able to inhibit/degrade the biofilm of S. aureus and E. coli.
The Mechanism of Manipulating Chirality and Chiral Sensing Based on Chiral Plexcitons in a Strong-Coupling Regime
Authors: Liang Xiongyu , Liang Kun , Deng Xuyan , He Chengmao , Zhou Peng , Li Junqiang , Qin Jianyu , Jin Lei , Yu Li
Published: April 2024

Manipulating plasmonic chirality has shown promising applications in nanophotonics, stereochemistry, chirality sensing, and biomedicine. However, to reconfigure plasmonic chirality, the strategy of constructing chiral plasmonic systems with a tunable morphology is cumbersome and complicated to apply for integrated devices. Here, we present a simple and effective method that can also manipulate chirality and control chiral light–matter interactions only via strong coupling between chiral plasmonic nanoparticles and excitons. This paper presents a chiral plexcitonic system consisting of L-shaped nanorod dimers and achiral molecule excitons. The circular dichroism (CD) spectra in our strong-coupling system can be calculated by finite element method simulations. We found that the formation of the chiral plexcitons can significantly modulate the CD spectra, including the appearance of new hybridized peaks, double Rabi splitting, and bisignate anti-crossing behaviors. This phenomenon can be explained by our extended coupled-mode theory. Moreover, we explored the applications of this method in enantiomer ratio sensing by using the properties of the CD spectra. We found a strong linear dependence of the CD spectra on the enantiomer ratio. Our work provides a facile and efficient method to modulate the chirality of nanosystems, deepens our understanding of chiral plexcitons in nanosystems, and facilitates the development of chiral devices and chiral sensing.
Pump-Driven Opto-Magnetic Properties in Semiconducting Transition-Metal Dichalcogenides: An Analytical Model
Authors: Rostami Habib , Cilento Federico , Cappelluti Emmanuele
Published: April 2024

Single-layer transition-metal dichalcogenides provide an unique intrinsic entanglement between the spin/valley/orbital degrees of freedom and the polarization of scattered photons. This scenario gives rise to the well-assessed optical dichroism observed by using both steady and time-resolved probes. In this paper, we provide compact analytical modeling of the onset of a finite Faraday/Kerr optical rotation upon shining with circularly polarized light. We identify different optical features displaying optical rotation at different characteristic energies, and we describe in an analytical framework the time-dependence of their intensities as a consequence of the main spin-conserving and spin-flip processes.