bluuebunny/crossref_metadata_2025 huggingface dataset:
An exploration of context and learning in endurance sports coaching
Authors: Kirkland Andrew , Cowley Joe
Published: April 2023

Introduction

This study explored contextual factors which influence coach learning of an international cohort of endurance sports coaches.

Methods

Following ethical approval, 839 coaches, 612 coached athletes and 8,352 non-coached athletes participated in the research. A critical realist research philosophy was adopted, in which self-completion surveys were developed in consultation with coaches and industry end-users.

Results and Discussion

The context was dominated by remote coaching practices and digital technology which shaped how coaches learn and thus, what it meant to be a coach. Unmediated learning sources were biophysically biased and largely delivered through marketised platforms designed to sell products. The study findings have broader implications within sport and education, in which it is suggested that remote coaching and learning platforms may sometimes create a sense of psycho-emotional detachment in which capacity for learning can be limited.
Growth, nodulation, yield, nitrogen uptake, and economics of lentil as influenced by sowing time, tillage, and management practices
Authors: Mukherjee Bishal , Kumar Naskar Manish , Nath Rajib , Atta Kousik , Visha Kumari Venugopalan , Banerjee Purabi , Alamri Saud , Patra Kiranmoy , Laing Alison M. , Skalicky Milan , Hossain Akbar
Published: April 2023

Crop management practices and variety are two very important parameters that decides the crop performance. A field experiment was carried out during the two consecutive rabi seasons of 2018–19 and 2019–20 to determine the impact of sowing timing, tillage operation, and variety on the growth, development, yield characteristics, and nitrogen uptake in lentil crops. The experiment was conducted in a split-split plot design with 3 replications comprising two different sowing conditions (S1: early sowing after harvesting of short duration kharif rice, S2: delayed sowing after harvesting of long duration kharif rice) in main plots, three different tillage operations (T1: Relay cropping, T2: Zero tillage, T3: Conventional tillage) in subplots and two different varieties (V1: short duration: L4717, V2: long period: Moitri) in subplots. The findings demonstrated a substantial interaction between sowing time, tillage, and variety on various growth and yield parameters of lentil crops. The early sowing of lentil crops (early November) yielded 4.8% more (1,105 kg ha−1) than late November sowing and adapting to the short-duration variety L4717 over the long-duration cultivar Moitri resulted in a yield increase of 5.9% (1,086 kg ha−1). Apart from providing a higher yield, it also provided an opportunity to take another crop like leafy vegetables. Among the three tillage practices adopted, conventional tillage produced the lowest yield (1,017 kg ha−1) in both experimental years. In contrast, a yield increase of 6.9% and 26.9% in relay cropping and zero tillage systems was observed, respectively. Early-sown lentils with no-tillage and a short-duration variety reached a certain phenophase faster than other combinations (life cycle: 96.2 and 98.7 days for lentils in both years). For both the sowing times, the growth parameters and the number of nodules plant−1 were highly correlated with nitrogen uptake at different stages of the life cycle. High net returns (Rs. 51,220 and 59,257) leading to higher benefit-cost ratios were observed under the treatment combination of early sowing + zero tillage + short duration variety. Therefore, the study found that short-duration lentil cultivars in combination with early sowing in the zero-tillage system are the best agronomic approach for the sustainability of lentil production after the monsoon rice harvest.
An integrated organic farming system: innovations for farm diversification, sustainability, and livelihood improvement of hill farmers
Authors: Layek Jayanta , Das Anup , Ansari Meraj A. , Mishra Vinay K. , Rangappa Krishnappa , Ravisankar Natesan , Patra Sandip , Baiswar Pankaj , Ramesh Thangam , Hazarika Samarendra , Panwar Azad S. , Devi Shidayaichenbi , Ansari Majhrool H. , Paramanik Bappa
Published: April 2023

Introduction

Organic farming is a promising solution for mitigating environmental burdens related to input-intensive agricultural practices. The major challenge in organic agriculture is the non-availability of large quantities of organic inputs required for crop nutrition and sustaining soil health, which can be resolved by efficient recycling of the available on- and off-farm resources and the integration of the components as per the specific locations.

Methods

An integrated organic farming system (IOFS) model comprising agricultural and horticultural crops, rainwater harvesting units, livestock components, and provisions for nutrient recycling was developed and disseminated in the adopted organic villages Mynsain, Pynthor, and Umden Umbathiang in the Ri-Bhoi District, Meghalaya, India, to improve the income and livelihood of farmers. Harvested rainwater in farm ponds andJalkundswas used for live-saving irrigation in the winter months and diversified homestead farming activities, such as growing high-value crops and rearing cattle, pigs, and poultry.

Results

Maize, french bean, potato, ginger, tomato, carrot, and chili yields in the IOFS model increased by 20%−30%, 40%−45%, 25%−30%, 33%−40%, 45%−50%, 37%−50%, and 27%−30%, respectively, compared with traditional practices. Some farmers produced vermicompost in vermibeds (made of high-density polyethylene) and cement brick chambers, generating 0.4−1.25 tons per annum. Two individual farmers, Mr. Jrill Makroh and Mrs. Skola Kurbah obtained net returns (without premium price) of Rs. 46,695 ± 418 and Rs. 31,102 ± 501 from their respective 0.27- and 0.21-ha IOFS models, which is equivalent to Rs. 172,944 ± 1,548/ha/year and Rs. 148,105 ± 2,385/ha/year, respectively. The net returns obtained from the IOFS models were significantly higher than those obtained from the farmers' practice of maize-fallow or cultivation of maize followed by vegetable (~30% of the areas). It is expected that, with the certification of organic products, the income and livelihood of the farmers will improve further over the years. While Mr. Jrill Makroh's model supplied 95.1%, 82.0%, and 96.0% of the total N, P2O5, and K2O, respectively, needed by the system, Mrs. Skola Kurbah's model supplied 76.0%, 68.6%, and 85.5% of the total N, P2O5, and K2O, respectively.

Discussion

Thus, IOFS models should be promoted among hill farmers so that they can efficiently recycle farm resources and increase their productivity, net returns, and livelihood while reducing their dependence on external farm inputs.
Comparison of the effects of negative pressure wound therapy and negative pressure wound therapy with instillation on wound healing in a porcine model
Authors: Tingting Sun , Xinyue Feng , Tiantian Yang , xiao An , Rui Li , Feng Lin , Daohong Liu , Zhirui Li , Guoqi Wang
Published: April 2023

Background

Negative pressure wound therapy with instillation (NPWTi) is a novel method based on standard negative pressure wound therapy (NPWT). This study aimed to compare the effects of standard NPWT and NPWTi on bioburden and wound healing in a Staphylococcus aureus (S.aureus) infected porcine model.

Methods

Green fluorescent protein-labeled S.aureus infected wounds were created on the back of porcine. Wounds were treated with NPWT or NPWT with instillation (saline). The tissue specimens were harvested on days 0 (12 h after bacterial inoculation), 2, 4, 6, and 8 at the center of wound beds. Viable bacterial counts, laser scanning confocal microscopy, PCR, western blot, and histological analysis were performed to assess virulence and wound healing.

Results

The bacterial count in the NPWTi group was lower than that of the NPWT group and the difference was statistically significant on day 2, day 4, day 6, and day 8 (P < 0.05). The expression levels of agrA, Eap, Spa, and Hla genes of the NPWTi group were significantly lower than that of the NPWT group on day 8 (P < 0.05). The bacterial invasion depth of the NPWTi group was significantly lower than that of the NPWT group on day 2, day 4, day 6, and day 8 (P < 0.05). Though the NPWTi group showed a significantly increased expression of bFGF and VEGF than that of the NPWT group in the early time (P < 0.05), NPWTi cannot lead to better histologic parameters than the NPWT group (P > 0.05).

Conclusion

Our results demonstrated that NPWTi induced a better decrease in bacterial burden and virulence compared with standard NPWT. These advantages did not result in better histologic parameters on the porcine wound model.
Left hemicolectomy and low anterior resection in colorectal cancer patients: Knight–griffen vs. transanal purse-string suture anastomosis with no-coil placement
Authors: Ammendola Michele , Filice Francesco , Battaglia Caterina , Romano Roberto , Manti Francesco , Minici Roberto , de'Angelis Nicola , Memeo Riccardo , Laganà Domenico , Navarra Giuseppe , Montemurro Severino , Currò Giuseppe
Published: April 2023

Background

Colorectal cancer (CRC) is considered one of the most frequent neoplasms of the digestive tract with a high mortality rate. Left hemicolectomy (LC) and low anterior resection (LAR) with minimally invasive laparoscopic and robotic approaches or with the open technique are the gold standard curative treatment.

Materials and methods

Seventy-seven patients diagnosed with CRC were recruited between September 2017 and September 2021. All patients underwent a preoperative staging with a full-body CT scan. The goal of this study was to compare both types of surgeries, LC-LAR LS with Knight–Griffen colorectal anastomosis and LC-LAR open with Trans-Anal Purse-String Suture Anastomosis (the TAPSSA group), by positioning a No-Coil transanal tube (SapiMed Spa, Alessandria, Italy), in terms of postoperative complications such as prolonged postoperative ileus (PPOI), anastomotic leak (AL), postoperative ileus (POI), and hospital stay.

Results

The patients were divided into two groups: the first with 39 patients who underwent LC and LAR in LS with Knight–Griffen anastomosis (Knight–Griffen group) and the second with 38 patients who underwent LC and LAR by the open technique with the TAPSSA group. Only one patient who underwent the open technique suffered AL. POI was 3.76 ± 1.7 days in the TAPSSA group and 3.07 ± 1.3 days in the Knight–Griffen group. There were no statistically significant differences in terms of AL and POI between the two different groups.

Conclusion

The important point that preliminarily emerged from this retrospective study was that the two different techniques showed similarities in terms of AL and POI, and therefore, all the advantages reported in the previous studies pertaining to No-Coil also hold good in this study regardless of the surgical technique used. However, randomized controlled trials are needed to confirm these findings.
Canine circovirus: An emerging or an endemic undiagnosed enteritis virus?
Authors: Gomez-Betancur Diana , Vargas-Bermudez Diana S. , Giraldo-Ramírez Sebastian , Jaime Jairo , Ruiz-Saenz Julian
Published: April 2023

Canine Circovirus (CanineCV) belongs to the family Circoviridae. It is an emerging virus described for the first time in 2011; since then, it has been detected in different countries and can be defined as worldwide distribution virus. CanineCV infects domestic and wild canids and is mainly related to hemorrhagic enteritis in canines. However, it has been identified in fecal samples from apparently healthy animals, where in most cases it is found in coinfection with other viral agents such as the canine parvovirus type-2 (CPV). The estimated prevalence/frequency of CanineCV has been variable in the populations and countries where it has been evaluated, reaching from 1 to 30%, and there are still many concepts to define the epidemiological characteristics of the virus. The molecular characterization and phylo-evolutive analyses that allow to postulate the wild origin and intercontinental distribution of the virus. This review focuses on the importance on continuing research and establish surveillance systems for this emerging virus.
Stress in wildlife: comparison of the stress response among domestic, captive, and free-ranging animals
Authors: Karaer Mina Cansu , Čebulj-Kadunc Nina , Snoj Tomaž
Published: April 2023

The stress response, which involves joint activity of the nervous and endocrine systems, is one of the basic adaptive mechanisms that ensures the survival of the individual. The activation of the sympathetic nervous system, the sympathetic-adrenal-medullary axis, and the hypothalamic–pituitary–adrenal axis enables organisms to respond to endogenous and exogenous challenges. Repeated short-term stress leads to long-term stress, which disrupts physiological homeostasis. Unlike domestic animals, wild animals are not protected from environmental and weather influences or treated for diseases. In addition, climate change, habitat fragmentation and loss, and urban stressors (such as light, noise and chemical pollution; xenobiotics; traffic; and buildings) affect individual wildlife and populations. In this review, we have attempted to depict the magnitude of the stress response in wildlife and related domestic animals as well as in captive and free-ranging animals. The intensity of the stress response can be estimated by determining the concentration of glucocorticoids in body fluids, tissues, and excreta. A comparison of results from different studies suggests that domestic animals have lower fecal and hair glucocorticoid concentrations than related wild animals. Additionally, fecal and hair glucocorticoid concentrations in captive animals are higher than in free-ranging animals of the same species. As there are limited data on this topic, we cannot draw definitive conclusions about glucocorticoid concentration and stress response. Further studies are needed to clarify these issues.
Research progress on the mechanisms underlying poultry immune regulation by plant polysaccharides
Authors: Zhao Ruo-Han , Yang Fang-Xiao , Bai Yi-Cheng , Zhao Jing-Ying , Hu Mei , Zhang Xin-Yan , Dou Teng-Fei , Jia Jun-Jing
Published: April 2023

With the rapid development of poultry industry and the highly intensive production management, there are an increasing number of stress factors in poultry production. Excessive stress will affect their growth and development, immune function, and induce immunosuppression, susceptibility to a variety of diseases, and even death. In recent years, increasing interest has focused on natural components extracted from plants, among which plant polysaccharides have been highlighted because of their various biological activities. Plant polysaccharides are natural immunomodulators that can promote the growth of immune organs, activate immune cells and the complement system, and release cytokines. As a green feed additive, plant polysaccharides can not only relieve stress and enhance the immunity and disease resistance of poultry, but also regulate the balance of intestinal microorganisms and effectively alleviate all kinds of stress faced by poultry. This paper reviews the immunomodulatory effects and molecular mechanisms of different plant polysaccharides (Atractylodes macrocephala Koidz polysaccharide, Astragalus polysaccharides, Taishan Pinus massoniana pollen polysaccharide, and alfalfa polysaccharide) in poultry. Current research results reveal that plant polysaccharides have potential uses as therapeutic agents for poultry immune abnormalities and related diseases.
Design of a Noncontact Torsion Testing Device Using Magnetic Levitation Mechanism
Authors: Ren Mengyi , Oka Koichi
Published: April 2023

To facilitate torsion testing in special environments, a noncontact torsion testing device, where a noncontact torque can be transmitted to a specimen, was developed using magnetic levitation technology. A permanent magnetic gear is used to produce noncontact torque. In addition, four electromagnets and four attractive-type permanent magnetic bearings were employed to realize levitation; in more detail, the four electromagnets actively stabilized two levitation degrees of freedom (DoFs), while the four attractive-type permanent magnetic bearings passively stabilized four DoFs. Furthermore, a plant model considering the effect caused by the four attractive-type permanent magnetic bearings was built for the two levitation DoFs requiring active control. Based on the plant model, two PD-controllers were designed. Moreover, a control simulation was conducted to obtain appropriate PD-gains. Finally, experiments further validated the feasibility of the whole scheme, and it was proven that the device can apply a 0.126 N·m torque to the specimen while maintaining levitation.
Structural Dynamic Characterization of a Modular Morphing Wing Exploiting Finite Elements and Taguchi Methodology
Authors: Mahmood Faisal , Hashemi Seyed M. , Alighanbari Hekmat
Published: April 2023

Detrimental environmental impacts due to the increasing demands of the aviation industry have gained tremendous global attention. With a potential fuel saving, along with high aerodynamic performance and maneuverability during different phases of a flight, adaptable wing design has become a viable alternative to its fixed-shape counterpart. A morphing wing design embraces, and can respond accordingly to, most of the flight condition variations effectively and efficiently. Despite these prospects, morphing wing design comes with some challenges due to its inherent complexity caused by an increased number of degrees of freedom. With the availability of various morphing parameters, the vibration signature of a morphing wing design plays a vital role in terms of its structural as well as aeroelastic characteristics. In the present paper, the dynamic characteristics of a re-configurable modular morphing wing developed in-house by a research team at Toronto Metropolitan University are investigated. This modular morphing wing, developed based on the idea of a parallel robot, consists of a number of structural elements connected to each other and to the wing ribs through eyebolt joints. Timoshenko bending beam theories, in conjunction with finite element methodology, are exploited. The free vibration of un-morphed (original) and morphed configurations undergoing multiple levels of sweep and spanwise morphing is presented through a design of experiment methodology.
From Gasotransmitter to Immunomodulator: The Emerging Role of Hydrogen Sulfide in Macrophage Biology
Authors: Cornwell Alex , Badiei Alireza
Published: April 2023

Hydrogen sulfide (H2S) has been increasingly recognized as a crucial inflammatory mediator in immune cells, particularly macrophages, due to its direct and indirect effects on cellular signaling, redox homeostasis, and energy metabolism. The intricate regulation of endogenous H2S production and metabolism involves the coordination of transsulfuration pathway (TSP) enzymes and sulfide oxidizing enzymes, with TSP’s role at the intersection of the methionine pathway and glutathione synthesis reactions. Additionally, H2S oxidation mediated by sulfide quinone oxidoreductase (SQR) in mammalian cells may partially control cellular concentrations of this gasotransmitter to induce signaling. H2S is hypothesized to signal through the posttranslational modification known as persulfidation, with recent research highlighting the significance of reactive polysulfides, a derivative of sulfide metabolism. Overall, sulfides have been identified as having promising therapeutic potential to alleviate proinflammatory macrophage phenotypes, which are linked to the exacerbation of disease outcomes in various inflammatory conditions. H2S is now acknowledged to have a significant influence on cellular energy metabolism by affecting the redox environment, gene expression, and transcription factor activity, resulting in changes to both mitochondrial and cytosolic energy metabolism processes. This review covers recent discoveries pertaining to the involvement of H2S in macrophage cellular energy metabolism and redox regulation, and the potential implications for the inflammatory response of these cells in the broader framework of inflammatory diseases.
Inflammation and Vitamin C in Women with Prenatal Depression and Anxiety: Effect of Multinutrient Supplementation
Authors: Carr Anitra C. , Bradley Hayley A. , Vlasiuk Emma , Pierard Hayley , Beddow Jessica , Rucklidge Julia J.
Published: April 2023

Elevated inflammation has been associated with adverse mood states, such as depression and anxiety, and antioxidant nutrients, such as vitamin C, have been associated with decreased inflammation and improved mood. In the current study comprising a cohort of pregnant women with depression and anxiety, we hypothesised that elevated inflammation would be associated with adverse mood states and inversely associated with vitamin C status and that multinutrient supplementation would optimise vitamin concentrations and attenuate inflammation. Sixty-one participants from the NUTRIMUM trial had blood samples collected between 12 and 24 weeks gestation (baseline) and following 12 weeks of daily supplementation with a multinutrient formula containing 600 mg of vitamin C or active placebo. The samples were analysed for inflammatory biomarkers (C-reactive protein (CRP) and cytokines) and vitamin C content and were related to scales of depression and anxiety. Positive correlations were observed between interleukin-6 (IL-6) and all of the mood scales administered (p < 0.05), including the Edinburgh Postnatal Depression Scale, the Clinical Global Impressions—Severity Scale, the Montgomery and Åsberg Depression Rating Scale, the Depression Anxiety Stress Scale 21, and the Generalized Anxiety Disorder-7 (GAD-7). CRP correlated weakly with GAD-7 (p = 0.05). There was an inverse correlation between CRP and the vitamin C status of the cohort (p = 0.045), although there was no association of the latter with the mood scales (p > 0.05). Supplementation with the multinutrient formula resulted in a significant increase in the vitamin C status of the cohort (p = 0.007) but did not affect the inflammatory biomarker concentrations (p > 0.05). In conclusion, greater systemic inflammation was associated with worse mood states; however, 12-week multinutrient supplementation did not alter inflammatory biomarker concentrations. Nevertheless, the vitamin C status of the cohort was improved with supplementation, which may aid pregnancy and infant outcomes.
Major Depressive Disorder and Oxidative Stress: A Review of Peripheral and Genetic Biomarkers According to Clinical Characteristics and Disease Stages
Authors: Ait Tayeb Abd El Kader , Poinsignon Vianney , Chappell Kenneth , Bouligand Jérôme , Becquemont Laurent , Verstuyft Céline
Published: April 2023

Major depressive disorder (MDD) is currently the main cause of disability worldwide, but its pathophysiology remains largely unknown, especially given its high heterogeneity in terms of clinical phenotypes and biological characteristics. Accordingly, its management is still poor. Increasing evidence suggests that oxidative stress, measured on various matrices such as serum, plasma or erythrocytes, has a critical role in MDD. The aim of this narrative review is to identify serum, plasma and erythrocyte biomarkers of oxidative stress in MDD patients according to disease stage and clinical features. Sixty-three articles referenced on PubMed and Embase between 1 January 1991, and 31 December 2022, were included. Modifications to antioxidant enzymes (mainly glutathione peroxidase and superoxide dismutase) in MDD were highlighted. Non-enzymatic antioxidants (mainly uric acid) were decreased in depressed patients compared to healthy controls. These changes were associated with an increase in reactive oxygen species. Therefore, increased oxidative damage products (principally malondialdehyde, protein carbonyl content and 8-hydroxy-2′-deoxyguanosine) were present in MDD patients. Specific modifications could be identified according to disease stages and clinical features. Interestingly, antidepressant treatment corrected these changes. Accordingly, in patients in remission from depression, oxidative stress markers were globally normalized. This narrative review suggests the particular interest of oxidative stress biomarkers for MDD care that may contribute to the heterogeneity of the disease and provide the opportunity to find new therapeutic targets.
Locally Activated Gated Neural Network for Automatic Music Genre Classification
Authors: Liu Zhiwei , Bian Ting , Yang Minglai
Published: April 2023

Automatic music genre classification is a prevailing pattern recognition task, and many algorithms have been proposed for accurate classification. Considering that the genre of music is a very broad concept, even music within the same genre can have significant differences. The current methods have not paid attention to the characteristics of large intra-class differences. This paper presents a novel approach to address this issue, using a locally activated gated neural network (LGNet). By incorporating multiple locally activated multi-layer perceptrons and a gated routing network, LGNet adaptively employs different network layers as multi-learners to learn from music signals with diverse characteristics. Our experimental results demonstrate that LGNet significantly outperforms the existing methods for music genre classification, achieving a superior performance on the filtered GTZAN dataset.
Potential for Therapeutic-Loaded Exosomes to Ameliorate the Pathogenic Effects of α-Synuclein in Parkinson’s Disease
Authors: Rademacher David J.
Published: April 2023

Pathogenic forms of α-synuclein (α-syn) are transferred to and from neurons, astrocytes, and microglia, which spread α-syn pathology in the olfactory bulb and the gut and then throughout the Parkinson’s disease (PD) brain and exacerbate neurodegenerative processes. Here, we review attempts to minimize or ameliorate the pathogenic effects of α-syn or deliver therapeutic cargo into the brain. Exosomes (EXs) have several important advantages as carriers of therapeutic agents including an ability to readily cross the blood–brain barrier, the potential for targeted delivery of therapeutic agents, and immune resistance. Diverse cargo can be loaded via various methods, which are reviewed herein, into EXs and delivered into the brain. Genetic modification of EX-producing cells or EXs and chemical modification of EX have emerged as powerful approaches for the targeted delivery of therapeutic agents to treat PD. Thus, EXs hold great promise for the development of next-generation therapeutics for the treatment of PD.
Synthetic Cationic Lipopeptide Can Effectively Treat Mouse Mastitis Caused by Staphylococcus aureus
Authors: Peng Jie , Lu Qiangsheng , Yuan Lvfeng , Zhang Hecheng
Published: April 2023

Mastitis caused by Staphylococcus aureus (S. aureus) in dairy cows is one of the most common clinical diseases in dairy cattle. Unfortunately, traditional antibiotic treatment has resulted in the emergence of drug-resistant strains of bacteria, making this disease more difficult to treat. Therefore, novel lipopeptide antibiotics are becoming increasingly important in treating bacterial diseases, and developing novel antibiotics is critical in controlling mastitis in dairy cows. We designed and synthesized three cationic lipopeptides with palmitic acid, all with two positive charges and dextral amino acids. The lipopeptides’ antibacterial activity against S. aureus was determined using MIC and scanning electron microscopy. The safety concentration range of lipopeptides for clinical usage was then estimated using the mouse erythrocyte hemolysis assay and CCK8 cytotoxicity. Finally, lipopeptides with high antibacterial activity and minimal cytotoxicity were selected for the treatment experiments regarding mastitis in mice. The observation of histopathological changes, bacterial tissue load and expression of inflammatory factors determined the therapeutic effects of lipopeptides on mastitis in mice. The results showed that all three lipopeptides displayed some antibacterial activity against S. aureus, with C16dKdK having a strong antibacterial impact and being able to treat the mastitis induced by S. aureus infection in mice within a safe concentration range. The findings of this study can be used as a starting point for the development of new medications for the treatment of mastitis in dairy cows.
Remineralization of Dentinal Lesions Using Biomimetic Agents: A Systematic Review and Meta-Analysis
Authors: Dawasaz Ali Azhar , Togoo Rafi Ahmad , Mahmood Zuliani , Ahmad Azlina , Thirumulu Ponnuraj Kannan
Published: April 2023

The objective of this article was to systematically provide an up-to-date review on the different methods of remineralizing human dentine using different biomimetic agents. The authors performed a systematic search within PubMed, Scopus, and Web of Science in addition to the grey literature in Google Scholar® using MeSH terms. The PICO question was P: human teeth dentinal sections; I: application of biomimetic remineralizing agents; C: other non-biomimetic approaches; O: extent of remineralization and physical properties of remineralized dentine. The initially identified studies were screened for titles and abstracts. Non-English articles, reviews, animal studies, studies involving the resin–dentine interface, and other irrelevant articles were then excluded. The other remaining full-text articles were retrieved. Bibliographies of the remaining articles were searched for relevant studies that could be included. A total of 4741 articles were found, and finally, 39 full-text articles were incorporated in the current systematic review. From these, twenty-six research studies used non-collagenous protein (NCP) analogs to biomineralize dentine, six studies used bioactive materials derived from natural sources, six studies used zinc hydroxyapatite, and one study used amelogenin peptide to induce hydroxyapatite formation on the surface of demineralized dentine. Additive effects of triclosan and epigenin were assessed when combined with commonly available NCPs. Overall, a moderate risk of bias was observed and, hence, the findings of the included studies could be acceptable. A meta-analysis of some similar studies was performed to assess the depth of remineralization and elastic modulus. Despite having high heterogeneity (I2 > 90), all the studies showed a significant improvement in biomimetic remineralization efficacy as compared to the control. All the included studies carried out a functional remineralization assessment and found a 90–98% efficacy in the extent of remineralization while the elastic modulus reached 88.78 ± 8.35 GPa, which is close to natural dentine. It is pertinent to note the limitations of these studies that have been carried out in vitro under controlled settings, which lack the effects of a natural oral environment. To conclude, the authors suggest that the biomimetic remineralization of dentine using NCP analogs, bioactive materials, and natural products carries significant potential in treating dentinal lesions; however, more long-term studies are needed to assess their clinical applications in vivo.
Kinematic Investigations of a Novel Flapping Actuation Design with Mutually Perpendicular 3 Cylindrical Joint Approach for FW-Drones
Authors: Singh Spoorthi , Zuber Mohammad , Hamidon Mohd Nizar , Azriff Basri Adi , Mazlan Norkhairunnisa , Ahmad Kamarul Arifin
Published: April 2023

The transmission mechanism of artificial flapping-wing drones generally needs low weight and the fewest interconnecting components, making their development challenging. The four-bar Linkage mechanism for flapping actuation has generally been used till now with complex and heavy connecting designs, but our proposed novel perpendicularly organized 3-cylindrical joint mechanism is designed to be unique and lighter weight with smooth functioning performance. The proposed prototype transforms the rotary motion of the motor into a specific angle of flapping movement, where the dimensions and specifications of the design components are proportional to the obtained flapping angle. Power consumption and flapping actuation can be monitored by adjusting the motor’s rotational speed to control the individual wing in this mechanism. The proposed mechanism consists of a crank with three slightly slidable cylindrical joints perpendicularly arranged to each other with a specified distance in a well-organized pattern to produce a flapping movement at the other end. In order to examine the kinematic attributes, a mathematical process approach is formulated, and kinematic simulations are performed using SIMSCAPE multibody MATLAB, PYTHON programming and COMPMECH GIM software. The proposed invention’s real-time test bench prototype model is designed, tested and analyzed for flapping validation.
Disseminating STEM Subjects and Ocean Literacy through a Bioinspired Toolkit
Authors: Costa Daniele , Screpanti Laura , Scaradozzi David
Published: April 2023

Over the last decade, education has been evolving to equip students with the fundamental skills required to cope with the challenges of sustainability and inclusivity, such as quality education, access to clean water, cultural heritage preservation and protection of marine life. Technology supports the learning process by providing useful tools that enrich the learning environment, encourage active participation, improve collaboration and prepare students for their future life. Educational Robotics is one of the most popular innovative methodologies that supports the development of many skills by assembling and programming robots in a meaningful way. In this paper, the authors aim at advancing their previous work in the field of Educational Robotics applied to the marine environment by proposing a novel bioinspired educational toolkit whose design and features support activities concerning sustainability, ocean literacy, as well as STEM subjects in kindergarten through to grade twelve education. Exploiting the established educational theories and methodologies underpinning Educational Robotics, the toolkit allows for marine-themed activities, as well promoting activities concerning STEM subjects. To explain the relevance of the toolkit, the authors present the robot design, the workshops that every teacher or student can explore as an Open Educational Resource (OERs), and the results of a case study. Interestingly, the latter shows that the use of the toolkit seems to have complemented the students’ initial keen interest in technology itself, with awareness about urgent issues related to the climate and the environment.
Engagement, Advance Care Planning, and Hospice Use in a Telephonic Nurse-Led Palliative Care Program for Persons Living with Advanced Cancer
Authors: Liddicoat Yamarik Rebecca , Chiu Laraine Ann , Flannery Mara , Van Allen Kaitlyn , Adeyemi Oluwaseun , Cuthel Allison M. , Brody Abraham A. , Goldfeld Keith S. , Schrag Deborah , Grudzen Corita R. ,   on behalf of the EMPallA Investigators
Published: April 2023

Persons living with advanced cancer have intensive symptoms and psychosocial needs that often result in visits to the Emergency Department (ED). We report on program engagement, advance care planning (ACP), and hospice use for a 6-month longitudinal nurse-led, telephonic palliative care intervention for patients with advanced cancer as part of a larger randomized trial. Patients 50 years and older with metastatic solid tumors were recruited from 18 EDs and randomized to receive nursing calls focused on ACP, symptom management, and care coordination or specialty outpatient palliative care (ClinicialTrials.gov: NCT03325985). One hundred and five (50%) graduated from the 6-month program, 54 (26%) died or enrolled in hospice, 40 (19%) were lost to follow-up, and 19 (9%) withdrew prior to program completion. In a Cox proportional hazard regression, withdrawn subjects were more likely to be white and have a low symptom burden compared to those who did not withdraw. Two hundred eighteen persons living with advanced cancer were enrolled in the nursing arm, and 182 of those (83%) completed some ACP. Of the subjects who died, 43/54 (80%) enrolled in hospice. Our program demonstrated high rates of engagement, ACP, and hospice enrollment. Enrolling subjects with a high symptom burden may result in even greater program engagement.
E. coli Phagelysate: A Primer to Enhance Nanoparticles and Drug Deliveries in Tumor
Authors: Ghambashidze Ketevan , Chikhladze Ramaz , Saladze Tamar , Hoopes P. Jack , Shubitidze Fridon
Published: April 2023

The tumor microenvironment (TME), where cancer cells reside, plays a crucial role in cancer progression and metastasis. It maintains an immunosuppressive state in many tumors and regulates the differentiation of precursor monocytes into M1 (anti-tumor)- and M2 (pro-tumor)-polarized macrophages, and greatly reduces anticancer drug and nanoparticle delivery. As a result, the effectiveness of recently developed chemo- and/or nanotechnology-mediated immune and magnetic nanoparticle hyperthermia (mNPH) therapies is inhibited significantly. One of the ways to overcome this limitation is to use E. coli phagelysate as a primer to modify the tumor microenvironment by switching tumor-associated M2 macrophages to anti-tumor M1 macrophages, and initiate the infiltration of tumor-associated macrophages (TAMs). Recently, bacteriophages and phage-induced lysed bacteria (bacterial phagelysates—BPLs) have been shown to be capable of modifying the tumor-associated environment. Phage/BPL-coated proteins tend to elicit strong anti-tumor responses from the innate immune system, prompting phagocytosis and cytokine release. It has also been reported that the microenvironments of bacteriophage- and BPL-treated tumors facilitate the conversion of M2-polarized TAMS to a more M1-polarized (tumoricidal) environment post-phage treatment. This paper demonstrates the feasibility and enhanced efficacy of combining E. coli phagelysate (EcPHL) and mNPH, a promising technology for treating cancers, in a rodent model. Specifically, we illustrate the EcPHL vaccination effect on the TME and mNP distribution in Ehrlich adenocarcinoma tumors by providing the tumor growth dynamics and histology (H&E and Prussian blue) distribution of mNP in tumor and normal tissue.
Introduction to the Special Issue on “Keratinocyte Carcinomas: Biology and Evolving Non-Invasive Management Paradigms”
Authors: González Salvador , Gill Melissa , Juarranz Ángeles
Published: April 2023

Keratinocyte carcinomas (KCs) are the most prevalent form of cancer worldwide, and their incidence is rising dramatically, with an increasing trend in recent years [...]
Adjuvant Therapy for Elderly Breast Cancer Patients after Breast-Conserving Surgery: Outcomes in Real World Practice
Authors: Rogowski Paul , Schönecker Stephan , Konnerth Dinah , Schäfer Annemarie , Pazos Montserrat , Gaasch Aurélie , Niyazi Maximilian , Boelke Edwin , Matuschek Christiane , Haussmann Jan , Braun Michael , Pölcher Martin , Würstlein Rachel , Harbeck Nadia , Belka Claus , Corradini Stefanie
Published: April 2023

We aimed to evaluate the standard of care of adjuvant radiotherapy (RT) after breast-conserving surgery (BCS) in elderly female patients (≥65 years) treated outside of clinical trials and to identify potential factors related to the omission of RT and the interaction with endocrine therapy (ET). All women treated with BCS at two major breast centers between 1998 and 2014 were evaluated. Data were provided by the Tumor Registry Munich. Survival analyses were conducted using the Kaplan–Meier method. Prognostic factors were identified using multivariate Cox regression analysis. The median follow-up was 88.4 months. Adjuvant RT was performed in 82% (2599/3171) of patients. Irradiated patients were younger (70.9 vs. 76.5 years, p < 0.001) and were more likely to receive additional chemotherapy (p < 0.001) and ET (p = 0.014). Non-irradiated patients more often had non-invasive DCIS tumors (pTis: 20.3% vs. 6.8%, p < 0.001) and did not undergo axillary surgery (no axillary surgery: 50.5% vs. 9.5%, p < 0.001). Adjuvant RT was associated with improved locoregional tumor control after BCS in invasive tumors (10-year local recurrence-free survival (LRFS): 94.0% vs. 75.1%, p < 0.001, 10-year lymph node recurrence-free survival (LNRFS): 98.1% vs. 93.1%, p < 0.001). Multivariate analysis confirmed significant benefits for local control with postoperative RT. Furthermore, RT led to increased locoregional control even in patients who received ET (10-year LRFS 94.8% with ET + RT vs. 78.1% with ET alone, p < 0.001 and 10-year LNRFS: 98.2% vs. 95.0%, p = 0.003). Similarly, RT alone had significantly better locoregional control rates compared to ET alone (10-year LRFS 92.6% with RT alone vs. 78.1% with ET alone, p < 0.001 and 10-year LNRFS: 98.0% vs. 95.0%, p = 0.014). The present work confirms the efficacy of postoperative RT for breast carcinoma in elderly patients (≥65 years) treated in a modern clinical setting outside of clinical trials, even in patients who receive ET.
Metal Oxide Semiconductor Gas Sensors for Lung Cancer Diagnosis
Authors: Li Guangyao , Zhu Xitong , Liu Junlong , Li Shuyang , Liu Xiaolong
Published: April 2023

Lung cancer is the most prevalent severe illness in both sexes and all ages and the leading cause of cancer-related deaths globally. Late-stage diagnosis is the primary cause of its high mortality rate. Therefore, the management of lung cancer needs early-stage screening. Breath analysis is a non-invasive, low-cost, and user-friendly approach to diagnosing lung cancer. Among the various types of breath sensors, MOS gas sensors are preferred due to their high gas responses, fast response times, robustness, and lower price. This review focuses on the critical role of MOS gas sensors in detecting VOCs in lung cancer patients’ exhaled breath. It introduces the basic working mechanism of MOS gas-sensitive materials, summarizes some high-performance MOS materials suitable for detecting potential lung cancer biomarkers and provides performance enhancement strategies. The review also briefly introduces the sensor array and its pattern recognition algorithm. Finally, we discuss the challenges in developing MOS gas sensors for lung cancer screening and present the prospect of using the e-nose for large-scale early lung cancer screening.
A Tracer Study on Child Participation in Child Councillor Programmes Aimed towards Development of the Child Friendly Cities Initiative
Authors: Alias Amelia , Mohamad Nasri Nurfaradilla , Awang Mohd Mahzan
Published: April 2023

The Child Friendly Cities Initiative introduced by UNICEF aims to help local governments realise child rights by utilising the UN Convention on the Rights of the Child as its foundation. Using Lundy’s model of child participation, which focuses on spaces, voice, audience, and influence, this study assesses young people’s participation as child councillors in two programmes in one city in Malaysia. Ten young people who were former child councillors in one state in Malaysia participated in this study. This study employed thematic analysis in analysing the data obtained using focus groups. Based on the data presented, it was clear that adult (the responsible party) understanding of meaningful child participation is still weak. This study offers substantial contributions to the limited body of literature on child participation in Malaysia by focusing on the difficulties of former child councillors in engaging in meaningful participation. Thus, more efforts (for example, by using participatory methods) are needed to educate the responsible party on the importance of addressing the power dynamic between children and adults so that children can participate effectively in decision-making processes.
Modern Paediatric Emergency Department: Potential Improvements in Light of New Evidence
Authors: Kula Roman , Popela Stanislav , Klučka Jozef , Charwátová Daniela , Djakow Jana , Štourač Petr
Published: April 2023

The increasing attendance of paediatric emergency departments has become a serious health issue. To reduce an elevated burden of medical errors, inevitably caused by a high level of stress exerted on emergency physicians, we propose potential areas for improvement in regular paediatric emergency departments. In an effort to guarantee the demanded quality of care to all incoming patients, the workflow in paediatric emergency departments should be sufficiently optimised. The key component remains to implement one of the validated paediatric triage systems upon the patient’s arrival at the emergency department and fast-tracking patients with a low level of risk according to the triage system. To ensure the patient’s safety, emergency physicians should follow issued guidelines. Cognitive aids, such as well-designed checklists, posters or flow charts, generally improve physicians’ adherence to guidelines and should be available in every paediatric emergency department. To sharpen diagnostic accuracy, the use of ultrasound in a paediatric emergency department, according to ultrasound protocols, should be targeted to answer specific clinical questions. Combining all mentioned improvements might reduce the number of errors linked to overcrowding. The review serves not only as a blueprint for modernising paediatric emergency departments but also as a bin of useful literature which can be suitable in the paediatric emergency field.
Phylogenomic Analysis of Two Species of Parasenecio and Comparative Analysis within Tribe Senecioneae (Asteraceae)
Authors: Liu Xiaofeng , Luo Junjia , Zhang Mingke , Wang Qiang , Liu Jie , Wu Die , Fu Zhixi
Published: April 2023

The genus of Parasenecio (Senecioneae) comprises about 70 species of high medicinal value. In this study, the plastomes of Parasenecio palmatisectus and P. latipes were newly sequenced using high-throughput sequencing technology and compared with those of eight other species in Senecioneae. The complete chloroplast (cp) genomes were 151,185 bp in P. latipes with 37.5% GC and 151,263 bp in P. palmatisectus with 37.5% GC. We predicted 133 genes, including 37 tRNA genes, 86 protein-coding genes, 8 rRNA genes, and 2 pseudogenes (ycf1 and rps19). A comparative genomic analysis showed that the complete cp genome sequences of Parasenecio species and their related species were relatively conserved. A total of 49 to 61 simple sequence repeats (SSRs) and 34 to 46 interspersed repeat sequences were identified in the 10 Senecioneae species of plastomes. Within the tribe Senecioneae, single-copy regions were more variable than inverted repeats regions, and the intergenic regions were more variable than the coding regions. Two genic regions (ycf1 and ccsA) and four intergenic regions (trnC-GCA-petN, ycf1-trnN-GUU, psaI-ycf4, and rpl32-trnL-UAG) were identified as highly valuable plastid markers. A phylogenetic analysis under maximum likelihood revealed that the two Parasenecio species are sister to the genera of Ligularia and Sinosenecio in the tribe Senecioneae. This study also contributes to the super-barcode, phylogenetic, and evolutionary studies of Parasenecio plants.
Discovery of the Puparia of a Whitefly Species Found on Malvaceae in the Pliocene Rajdanda Formation, Jharkhand, Eastern India
Authors: Drohojowska Jowita , Evans Gregory A. , Khan Mahasin Ali , Hazra Taposhi , Szwedo Jacek
Published: April 2023

A new genus and species of fossil whitefly, Praealeurolobus indicus gen. et sp. nov., is described and illustrated based on puparia found on leaves of a malvaceous plant from the Pliocene Rajdanda Formation in Eastern India. It is the first record of whitefly puparia on leaves from the Neogene deposits of Asia and the second taxon formally named and described from such adpression fossils. The cephalothorax of some of the puparia has a large, irregularly shaped hole with jagged edges, typical of the emergence holes of parasitoids of modern-day whiteflies and represents the earliest record of parasitism on whiteflies. This finding enriches our knowledge of the fossil record of Aleyrodidae in connection with allied fossils and provides new insight into the ecological interactions of the palaeobiota and taphonomy of fossils preserved in the Pliocene Rajdanda Formation.