MicroRNA expression profile in the diagnostic substrate of primary cutaneous T cell non-Hodgkin’s lymphoma
Authors:   Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Veryaskina Yu.А. , Kovynev I.B. ,   Novosibirsk State Medical University, Pakhomova V.V. ,   Novosibirsk Regional Clinical Skin and Venereal Diseases Dispensary, Novosibirsk, Russia, Titov S.Е. ,   Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences; Vector-Best, Novosibirsk, Russia, Voitko М.S. ,   Novosibirsk State Medical University, Tsigulev K.S. ,   Novosibirsk State Medical University, Onipchenko V.V. ,   Novosibirsk Regional Clinical Skin and Venereal Diseases Dispensary, Novosibirsk, Russia
Published: December 2024

Introduction. Primary cutaneous T cell lymphomas (CTCL) are a heterogeneous group of extranodal non-Hodgkin’s lymphomas that are locally limited to the skin at diagnosis. The diagnosis of CTCL is often difficult, since at certain stages of development, this hematological malignancy has clinical similarities with non-neoplastic dermatological diseases such as chronic eczematous dermatitis, psoriasis, lichen ruber, or fungal infections. Both genetic and epigenetic factors, in particular microRNAs (miRNAs), are involved in the pathogenesis of CTCL.
Aim. To identify the miRNAs that differentiate CTCL from benign skin lesions (BSL) and determine their role in genetic pathways involved in the development of CTCL.
Materials and methods. Real-time reverse transcription PCR was used to analyze the expression levels of miRNA-181a, -155, -574, -148b, -191, -26a, -21, -124, -221, -200b, -20a, -92a, -145, let-7a, and let-7d in 5 formalin-fixed paraffin-embedded skin biopsy samples from patients with CTCL and 20 skin biopsy samples from patients with psoriasis (control group).
Introduction. Primary cutaneous T cell lymphomas (CTCL) are a heterogeneous group of extranodal non-Hodgkin’s lymphomas that are locally limited to the skin at diagnosis. The diagnosis of CTCL is often difficult, since at certain stages of development, this hematological malignancy has clinical similarities with non-neoplastic dermatological diseases such as chronic eczematous dermatitis, psoriasis, lichen ruber, or fungal infections. Both genetic and epigenetic factors, in particular microRNAs (miRNAs), are involved in the pathogenesis of CTCL.
Aim. To identify the miRNAs that differentiate CTCL from benign skin lesions (BSL) and determine their role in genetic pathways involved in the development of CTCL.
Materials and methods. Real-time reverse transcription PCR was used to analyze the expression levels of miRNA-181a, -155, -574, -148b, -191, -26a, -21, -124, -221, -200b, -20a, -92a, -145, let-7a, and let-7d in 5 formalin-fixed paraffin-embedded skin biopsy samples from patients with CTCL and 20 skin biopsy samples from patients with psoriasis (control group).
Results. Comparative analysis of miRNA expression levels between CTCL and BSL samples showed a statistically significant increase in the levels of miRNA-181a, -155, -574, -148b and -191 in tumor samples (p < 0.05). ROC analysis showed that miRNA-155 and miRNA-181a are highly sensitive and specific markers for the diagnosis of CTCL.
Conclusion. Analysis of miRNA expression levels may be a promising tool for the differential diagnosis between dermatotropic lymphoid hematological malignancies and non-hematological skin diseases.