bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Defect Size on Bone Healing in Critical-Size Bone Defects Investigated on a Rat Femur Defect Model Comparing Two Treatment Methods
Authors: Kammerer Andreas , Hartmann Frederik Alexander , Nau Christoph , Leiblein Maximilian , Schaible Alexander , Neijhoft Jonas , Henrich Dirk , Verboket René , Janko Maren
Published: March 2024

Critical-size bone defects up to 25 cm can be treated successfully using the induced membrane technique established by Masquelet. To shorten this procedure, human acellular dermis (HAD) has had success in replacing this membrane in rat models. The aim of this study was to compare bone healing for smaller and larger defects using an induced membrane and HAD in a rat model. Using our established femoral defect model in rats, the animals were placed into four groups and defects of 5 mm or 10 mm size were set, either filling them with autologous spongiosa and surrounding the defect with HAD or waiting for the induced membrane to form around a cement spacer and filling this cavity in a second operation with a cancellous bone graft. Healing was assessed eight weeks after the operation using µ-CT, histological staining, and an assessment of the progress of bone formation using an established bone healing score. The α-smooth muscle actin used as a signal of blood vessel formation was stained and counted. The 5 mm defects showed significantly better bone union and a higher bone healing score than the 10 mm defects. HAD being used for the smaller defects resulted in a significantly higher bone healing score even than for the induced membrane and significantly higher blood vessel formation, corroborating the good results achieved by using HAD in previous studies. In comparison, same-sized groups showed significant differences in bone healing as well as blood vessel formation, suggesting that 5 mm defects are large enough to show different results in healing depending on treatment; therefore, 5 mm is a viable size for further studies on bone healing.
Bibliometric Overview on T-Cell Intracellular Antigens and Their Pathological Implications
Authors: Ramos-Velasco Beatriz , Naranjo Rocío , Izquierdo José M.
Published: March 2024

T-cell intracellular antigen 1 (TIA1) and TIA1-like/related protein (TIAL1/TIAR) are two members of the classical family of RNA binding proteins. Through their selective interactions with distinct RNAs and proteins, these multifunctional regulators are involved in chromatin remodeling, RNA splicing and processing and translation regulation, linking them to a wide range of diseases including neuronal disorders, cancer and other pathologies. From their discovery to the present day, many studies have focused on the behavior of these proteins in order to understand their impact on molecular and cellular processes and to understand their relationship to human pathologies. The volume of research on these proteins in various fields, including molecular biology, biochemistry, cell biology, immunology and cancer, has steadily increased, indicating a growing interest in these gene expression regulators among researchers. This information can be used to know the most productive institutions working in the field, understand the focus of research, identify key areas of involvement, delve deeper into their relationship and impact on different diseases, and to establish the level of study associated with them.
Characterization of Skeletal Muscle Regeneration Revealed a Novel Growth Network Induced by Molecular Acupuncture-like Transfection
Authors: Zádor Ernő
Published: March 2024

The low efficiency of in vivo transfection of a few fibres revealed a novel tissue network that temporally amplified growth stimulation in the entire regenerating rat soleus muscle. This acupuncture-like effect was demonstrated when the fibres began to grow after complete fibre degradation, synchronous inflammation, myoblast and myotube formation. Neonatal sarcoplasmic/endoplasmic reticulum ATPase (SERCA1b) was first detected in this system. The neonatal, fast and slow SERCA isoforms displayed consequent changes with innervation and differentiation, recapitulating events in muscle development. In vivo transfection of myotubes with plasmids expressing dominant negative Ras or a calcineurin inhibitor peptide (Cain/cabin) proved that expression of the slow myosin heavy chain and the slow muscle type SERCA2a are differentially regulated. In vivo transfection of a few nuclei of myotubes with dnRas or SERCA1b shRNA stimulated fibre size growth in the whole regenerating muscle but only until the full size had been reached. Growth stimulation by Ras and SERCA1b antisense was abolished by co-transfection of Cain or with perimuscular injection of IL4 antibody. This revealed a novel signalling network resembling scale-free networks which, starting from transfected fibre myonuclei as “hubs”, can amplify growth stimulation uniformly in the entire regenerating muscle.
Solid Phase Oligo-DNA Extraction from Complex Medium Using an Aminated Graphene/Nitrocellulose Membrane Hybrid
Authors: Toader Georgian Alin , Grigorean Valentin Titus , Ionita Mariana
Published: March 2024

A hybrid material, consisting of commercially available nitrocellulose (NC) membrane non-covalently modified with amino-polyethylene glycol functionalized reduced graphene oxide (NH2-PEG-rGO) nanoparticles, was successfully synthesized for oligonucleotide extraction. Fourier Transform Infrared Spectroscopy (FTIR) confirmed the modification of the NC membrane, revealing characteristic peaks of both compounds, i.e., NC and NH2-PEG-rGO. Scanning Electron Microscopy (SEM) exhibited morphological changes in the NC/NH2-PEG-rGO hybrid membrane, marked by the introduction of NH2-PEG-rGO particles, resulting in a distinctly smothered surface compared to the porous surface of the NC control membrane. Wettability assays revealed hydrophobic behavior for the NC/NH2-PEG-rGO hybrid membrane, with a water contact angle exceeding 90°, contrasting with the hydrophilic behavior characterized by a 16.7° contact angle in the NC membrane. The performance of the NC/NH2-PEG-rGO hybrid membrane was evaluated for the extraction of ssDNA with fewer than 50 nucleotides from solutions containing various ionic species (MnCl2, MgCl2, and MnCl2/MgCl2). The NC/NH2-PEG-rGO hybrid membrane exhibited optimal performance when incubated in MgCl2, presenting the highest fluorescence emission at 525 relative fluorescence units (r.f.u.). This corresponds to the extraction of approximately 610 pg (≈13%) of the total oligo-DNA, underscoring the efficacy of the pristine material, which extracts 286 pg (≈6%) of oligo-DNA in complex solutions.
Towards Uncovering the Role of Incomplete Penetrance in Maculopathies through Sequencing of 105 Disease-Associated Genes
Authors: Hitti-Malin Rebekkah J. , Panneman Daan M. , Corradi Zelia , Boonen Erica G. M. , Astuti Galuh , Dhaenens Claire-Marie , Stöhr Heidi , Weber Bernhard H. F. , Sharon Dror , Banin Eyal , Karali Marianthi , Banfi Sandro , Ben-Yosef Tamar , Glavač Damjan , Farrar G. Jane , Ayuso Carmen , Liskova Petra , Dudakova Lubica , Vajter Marie , Ołdak Monika , Szaflik Jacek P. , Matynia Anna , Gorin Michael B. , Kämpjärvi Kati , Bauwens Miriam , De Baere Elfride , Hoyng Carel B. , Li Catherina H. Z. , Klaver Caroline C. W. , Inglehearn Chris F. , Fujinami Kaoru , Rivolta Carlo , Allikmets Rando , Zernant Jana , Lee Winston , Podhajcer Osvaldo L. , Fakin Ana , Sajovic Jana , AlTalbishi Alaa , Valeina Sandra , Taurina Gita , Vincent Andrea L. , Roberts Lisa , Ramesar Raj , Sartor Giovanna , Luppi Elena , Downes Susan M. , van den Born L. Ingeborgh , McLaren Terri L. , De Roach John N. , Lamey Tina M. , Thompson Jennifer A. , Chen Fred K. , Tracewska Anna M. , Kamakari Smaragda , Sallum Juliana Maria Ferraz , Bolz Hanno J. , Kayserili Hülya , Roosing Susanne , Cremers Frans P. M.
Published: March 2024

Inherited macular dystrophies (iMDs) are a group of genetic disorders, which affect the central region of the retina. To investigate the genetic basis of iMDs, we used single-molecule Molecular Inversion Probes to sequence 105 maculopathy-associated genes in 1352 patients diagnosed with iMDs. Within this cohort, 39.8% of patients were considered genetically explained by 460 different variants in 49 distinct genes of which 73 were novel variants, with some affecting splicing. The top five most frequent causative genes were ABCA4 (37.2%), PRPH2 (6.7%), CDHR1 (6.1%), PROM1 (4.3%) and RP1L1 (3.1%). Interestingly, variants with incomplete penetrance were revealed in almost one-third of patients considered solved (28.1%), and therefore, a proportion of patients may not be explained solely by the variants reported. This includes eight previously reported variants with incomplete penetrance in addition to CDHR1:c.783G>A and CNGB3:c.1208G>A. Notably, segregation analysis was not routinely performed for variant phasing—a limitation, which may also impact the overall diagnostic yield. The relatively high proportion of probands without any putative causal variant (60.2%) highlights the need to explore variants with incomplete penetrance, the potential modifiers of disease and the genetic overlap between iMDs and age-related macular degeneration. Our results provide valuable insights into the genetic landscape of iMDs and warrant future exploration to determine the involvement of other maculopathy genes.
Measuring Occupational Well-Being Indicators: Scale Construction and Validation
Authors: Daovisan Hanvedes , Intarakamhang Ungsinun
Published: March 2024

The purpose of this study is to carry out the scale development of occupational well-being (OWB) (affective, professional, social, cognitive, psychological and psychosomatic well-being) in Laos. Using multiple sampling data, we developed a valid OWB scale with a large Laotian sample (n = 1745). The validity of the OWB-47 scale was analyzed using exploratory factor analysis (EFA). Cross-validity, the initial model, and confirmatory factor analysis (CFA) were performed using Stata 19 to assess the validity of the scale development. Consistent with the valid model, the CFA revealed a unidimensional structure in the OWB scale. The initial measurement of the OWB scale was significantly correlated with the measure of the six-dimensional model. Regarding the full model testing, the CFA model was developed to test the validity of the OWB-47 scale, suggesting the acceptability of the fit model.
Multidimensional Evaluation of Traditional Villages in Jiangnan Region, China: Spatial Pattern, Accessibility and Driving Factors
Authors: Zhang Yi , Tian Zheng , Du Jiacheng , Bi Shibo
Published: March 2024

Assessment of the spatial distribution and accessibility of traditional villages is closely related to their development. However, the impacts of spatial heterogeneity on the accessibility of traditional villages remain largely unknown. A total of 644 national-level traditional villages in the Jiangnan region were used to explore the spatial heterogeneity impact with a geographically weighted regression (GWR) model. We found: (1) spatially, the villages exhibit a predominant aggregation distribution pattern with significant local spatial disparities (R < 1, p < 0.01), predominantly originating from the Song and Ming dynasties (59.21%). Their clustering shifts from northeast to southwest, with over 70% of the villages located on slopes ranging from 0° to 20° and within 1 km of water. (2) The accessibility of these villages is generally low, with 85.66% being accessible within 200 ~ 300 min; it decreases concentrically outwards from Changzhou and exhibits clustering autocorrelation characteristics (Moran’s I > 0, Z > 2.58). (3) Road network density, elevation, and slope are significantly correlated with accessibility (p < 0.05), demonstrating pronounced spatial heterogeneity in their effects, with these factors collectively explaining approximately 85% of the accessibility levels. These findings provide a basis for comprehensive planning and categorized strategies for traditional villages.
Systematic Calculation of Yield and Failure Curvatures of Reinforced Concrete Cross-Sections
Authors: Bellos John , Konstantinidis Apostolos
Published: March 2024

This paper examines and provides a robust solution to the problem of yield and failure curvatures of reinforced concrete (RC) cross-sections, taking into account cracking. At the same time, it calculates the corresponding necessary reinforcement or the moment of resistance in both yield and failure limit states. Computationally, the problem of determining the actual curvatures is reduced to the bending design problem of the cross-section in the yield and failure limit states. This study shows the researcher and the designer how to systematically calculate the strains for different concrete and steel grades and for standard or random cross-sections. This complex process is quite necessary to determine the respective curvatures. The main concept is presented with an emphasis on the “solution regions” as well as the critical cases of the “asymptotic regions”, both in yield and failure limit states. Our wide-ranging research on RC element design under biaxial bending with axial force for both yield and failure limit states has been completed and validated via sophisticated algorithms and is available for publication.
Incorporation of Waste in Thermal Mortars—A Literature Review
Authors: Barros Amanda , Barreira Eva , Maia Lino , Lopes Maria Lurdes
Published: March 2024

Innovation in construction plays a fundamental role in helping us face current challenges, namely the reduction in energy consumption, the mitigation of the effects of climate change, the depletion of resources, and the generation of waste. Regarding the built environment, improving the thermal properties of the building envelope is one of the growing needs to reduce energy consumption in the building sector. In this context, thermal mortars have been a trend in the construction industry in recent years due to their ability in reducing heat transfer through the building envelope. On the other hand, the addition of waste has been studied as an alternative to improve the thermal properties of mortars and reduce the consumption of primary materials in the construction sector. This work aims to carry out a detailed review regarding the incorporation of waste in thermal mortars through the application of scientometric data analysis and a systematic literature review. To this end, the different residues incorporated into thermal mortars and the various percentages and forms of incorporation were identified throughout the publications gathered in this review. The most studied properties regarding the thermal mortars with the addition of waste were also the subject of study. A comprehensive database of thermal mortars with the incorporation of waste is presented, in which the objectives of the studies, the wastes and forms of incorporation and the measured properties are highlighted. The main results of the analysed researches are deeply discussed and the gaps in this area of the knowledge are identified to point out new directions and possible perspectives for future studies in the field of thermal mortars incorporating waste.
Harnessing ctDNA in Advanced Melanoma: A Promising Tool for Informed Clinical Decisions
Authors: Pikturniene Rugile , Cesas Alvydas , Jarmalaite Sonata , Razbadauskas Arturas , Urbonas Vincas
Published: March 2024

Cutaneous melanoma, an aggressive malignancy, has undergone significant transformation in clinical management with the introduction of immune checkpoint inhibitors (ICIs) and targeted therapies. Current monitoring methods, such as imaging scans, present limitations, prompting exploration of alternative biomarkers. This review comprehensively explores the role of circulating tumor DNA (ctDNA) in advanced melanoma, covering technical aspects, detection methods, and its prognostic and predictive value. Recent findings underscore ctDNA’s potential applications and implications in clinical practice. This review emphasizes the need for precise and dynamic biomarkers in melanoma care, positioning ctDNA as a promising blood-based tool for prognosis, treatment response, and resistance mechanisms. The technical nuances of ctDNA detection, association with melanoma mutations, and its role in guiding therapeutic decisions for immunotherapy and targeted therapy underscore its multifaceted utility, marking a paradigm shift in clinical decision-making and offering a promising trajectory for personalized and informed care in advanced melanoma.
Protein Stability Regulation in Osteosarcoma: The Ubiquitin-like Modifications and Glycosylation as Mediators of Tumor Growth and as Targets for Therapy
Authors: Di Gregorio Jacopo , Di Giuseppe Laura , Terreri Sara , Rossi Michela , Battafarano Giulia , Pagliarosi Olivia , Flati Vincenzo , Del Fattore Andrea
Published: March 2024

The identification of new therapeutic targets and the development of innovative therapeutic approaches are the most important challenges for osteosarcoma treatment. In fact, despite being relatively rare, recurrence and metastatic potential, particularly to the lungs, make osteosarcoma a deadly form of cancer. In fact, although current treatments, including surgery and chemotherapy, have improved survival rates, the disease’s recurrence and metastasis are still unresolved complications. Insights for analyzing the still unclear molecular mechanisms of osteosarcoma development, and for finding new therapeutic targets, may arise from the study of post-translational protein modifications. Indeed, they can influence and alter protein structure, stability and function, and cellular interactions. Among all the post-translational modifications, ubiquitin-like modifications (ubiquitination, deubiquitination, SUMOylation, and NEDDylation), as well as glycosylation, are the most important for regulating protein stability, which is frequently altered in cancers including osteosarcoma. This review summarizes the relevance of ubiquitin-like modifications and glycosylation in osteosarcoma progression, providing an overview of protein stability regulation, as well as highlighting the molecular mediators of these processes in the context of osteosarcoma and their possible targeting for much-needed novel therapy.
Anxiety and Depression and Associated Risk Factors among Outpatients with Systemic Lupus Erythematosus: Eastern Province, Saudi Arabia
Authors: Hasan Manal Ahmed , Almogaliq Wasayf Salman , Alhanabi Fatimah Habib , Aldrazi Hebah Abbas , Alkhouzaie Moath Thamer , Albukhari Raed , Alqatari Safi , Al-Abdulwahab Abdullah A. , AlZuhair Hajer Musaab , Al-Hariri Mohammed T.
Published: March 2024

Background: Although mood disorders are prevalent among systemic lupus erythematosus (SLE) patients, they are usually underrecognized. This study aimed to estimate the prevalence of anxiety and depression among Saudi SLE patients. Methods: This cross-sectional study was conducted among SLE patients from July 2022 to June 2023 in the Eastern Province of Saudi Arabia. A self-reported questionnaire was used to collect the data through validated tools including the Hamilton Anxiety Rating Scale-A and the Beck Depression Inventory score. Results: There were 133 females (91.7%) and 12 males (8.3%) included in this study. Based on the HAM-A score, 45.5% of participants had an anxiety disorder, and according to the BDI score, 46.2% had a depression disorder. Anxiety and depression were significantly associated with a longer duration of SLE, unemployment status, smoking, and the presence of comorbidities. Moreover, the present study found a significant association between depression and male gender. Conclusion: This study found that Saudi SLE patients have a high prevalence of both anxiety and depression. Therefore, SLE patients should be screened for neuropsychiatric disorders during routine follow-ups and managed as early as possible.
Rotator Cuff Muscle Imbalance in Patients with Chronic Anterior Shoulder Instability
Authors: Kim Du-Han , Kim Ji-Hoon , Cho Chul-Hyun
Published: March 2024

(1) Background: Both intra-articular pathologies and muscle imbalance can be a cause of shoulder instability. The purpose of this study is to examine the cross-sectional areas of the rotator cuff muscle in patients with acute and chronic anterior shoulder instability and to determine the associations between imbalance and chronicity of the rotator cuff. (2) Methods: Patients with confirmed dislocation of the anterior shoulder were included. The patients were divided into two groups according to the time between the initial dislocation event and when MRI imaging was performed Measurements of the rotator cuff muscle areas were performed in the scapular Y view and glenoid face view using MRI. (3) Results: A total of 56 patients were enrolled. In the Y view, a larger area of supraspinatus muscle was observed in the chronic group compared with the acute group (17.2 ± 2.3% vs. 15.6 ± 2.2%, p = 0.006). However, a smaller area of subscapularis muscle was observed in the chronic group (47.1 ± 3.5% vs. 49.6 ± 5.3%, p = 0.044). Using the glenoid face view, a larger area of supraspinatus muscle was observed in the chronic group than in the acute group (18.5 ± 2.5% vs. 15.8 ± 2.2%, p < 0.001). However, a smaller area of subscapularis muscle was observed in the chronic group (41.6 ± 3.2% vs. 45.6 ± 4.4%, p < 0.001). (4) Conclusion: Larger areas of supraspinatus muscle compared with acute instability were observed in patients with chronic anterior shoulder instability. In contrast, a smaller area of subscapularis muscle was observed in the chronic group.
Exploring the Possible Impact of Oral Nutritional Supplements on Children’s Oral Health: An In Vitro Investigation
Authors: Anticona Cynthia , Hansson Lena , Johansson Ingegerd , Lif Holgerson Pernilla
Published: March 2024

Eight pediatric oral nutritional supplements (ONSs) and 0.5% fat bovine milk were examined in vitro regarding their effect on the adhesion of three caries-related bacteria, Streptococcus mutans (strain CCUG 11877T), Lactobacillus gasseri (strain CCUG 31451), and Scardovia wiggsiae (strain CCUG 58090), to saliva-coated hydroxyapatite, as well as their pH and capacity to withstand pH changes. Bacteria were cultivated and radiolabeled. The adhesion assays used synthetic hydroxyapatite coated with whole or parotid saliva. Measurements of pH and titration of the products with HCl and NaOH were conducted in triplicate. Three ONSs promoted the S. mutans adhesion to saliva-coated hydroxyapatite (increase from 35% to >200%), supporting caries risk enhancement. S. wigssiae and L. gasseri adhered only to one and no ONS, respectively. Most supplements had limited buffering capacity to counteract acidification changes, suggesting their low capacity to neutralize acids, and one ONS showed a significant capacity to counteract basic changes, suggesting a high erosive potential. S. mutans adhesion was influenced by the ONS pH and volume NaOH added to reach pH 10. L. gasseri and S. wiggsiae adhesion was influenced by the ONSs’ carbohydrate and fat content. Interdisciplinary efforts are needed to increase awareness and prevent the possible negative impact of ONSs on children’s oral health.
The Future of Postsecondary Education in the Age of AI
Authors: Essa Alfred
Published: March 2024

This paper examines a possible future for postsecondary education in the age of AI. The consensus view among economists is that AI is a general purpose technology (GPT), similar to the steam engine, electricity, and the internet. As a GPT, AI will be the main driver of innovation for the foreseeable future in most sectors of the economy, including education. As AI evolves, it holds the promise of fundamentally redefining the educational landscape, influencing not only current practices in institutional management and pedagogy but also shaping future trends in learning, evaluation, and accreditation. While traditional college-aged students have received significant attention in educational studies, this paper emphasizes the needs of adult learners as lifelong learners and explores how AI-driven innovations can enhance their educational experiences, offering personalized and flexible learning solutions. This paper also argues that a dramatic breakthrough is needed in the cost–value equation for education to support workforce development and lifelong learning.
Culturally Responsive Middle Leadership for Equitable Student Outcomes
Authors: Highfield Camilla , Webber Melinda , Woods Rachel
Published: March 2024

Middle leaders are important conduits for school policy and are often required to lead, champion and monitor change initiatives in their departments. This paper examines secondary school middle leaders’ self-reported theories, promotion and leadership of culturally responsive teaching approaches for student equity. The study participants (n = 170) are curriculum leaders in state secondary schools throughout New Zealand, serving a range of diverse populations, including Indigenous Māori students. The quantitative and qualitative data for this project were thematically analysed to inquire into middle leaders’ articulation of their culturally responsive theories, practices, and pedagogical and curriculum leadership to assist members of their departments in supporting Māori student educational success. The study found that although middle leaders could clearly articulate culturally responsive, sustaining and revitalising pedagogies and leadership in alignment with government policies and expectations, they were less clear about the measures they used to evidence these practices. In addition, less than 30% of the middle leader participants mentioned academic achievement as an element of Māori student success, and few mentioned the importance of systematic monitoring of achievement data, or using them to support better learning outcomes for Māori students (184).
Input Voltage-Level Driven Split-Input Inverter Level Shifter for Nanoscale Applications
Authors: Gundala Srinivasulu , Basha Mohammed Mahaboob , Madhurima Virupakshi , Stan Ovidiu Petru
Published: March 2024

A level shifter (LS) appears to be highly efficient and effective in solving voltage contentions between deep sub-threshold and core voltage levels. An input voltage-level driven split-input inverter that can create common unconnected PMOS and NMOS transistors for the input inverter is proposed, which is powered and used at the input stage to achieve maximum conversion efficiency. Layout and simulation results across different corners have demonstrated that the proposed LS is highly useful for cutting-edge nanoscale applications. It can up-convert voltage from 0.2 V to 1.2 V and down-convert from 1.2 V to 0.2 V @ 1 MHz input pulse, with a level-up or level-down mean switching delay of 1.3 ns, and a power of 9.5 nW. Moreover, the LS occupies an area of 8 μm2, which is a reasonably compact size compared to the typical LS designs. Overall, the proposed voltage LS design is an efficient and effective solution that could have an ample range of applications in IoT and biomedical, wireless sensor networks.
Crafting Creative Melodies: A User-Centric Approach for Symbolic Music Generation
Authors: Dadman Shayan , Bremdal Bernt Arild
Published: March 2024

Composing coherent and structured music is one of the main challenges in symbolic music generation. Our research aims to propose a user-centric framework design that promotes a collaborative environment between users and knowledge agents. The primary objective is to improve the music creation process by actively involving users who provide qualitative feedback and emotional assessments. The proposed framework design constructs an abstract format in which a musical piece is represented as a sequence of musical samples. It consists of multiple agents that embody the dynamics of musical creation, emphasizing user-driven creativity and control. This user-centric approach can benefit individuals with different musical backgrounds, encouraging creative exploration and autonomy in personalized, adaptive environments. To guide the design of this framework, we investigate several key research questions, including the optimal balance between system autonomy and user involvement, the extraction of rhythmic and melodic features through musical sampling, and the effectiveness of topological and hierarchical data representations. Our discussion will highlight the different aspects of the framework in relation to the research questions, expected outcomes, and its potential effectiveness in achieving objectives. Through establishing a theoretical foundation and addressing the research questions, this work has laid the groundwork for future empirical studies to validate the framework and its potential in symbolic music generation.
A Multi-Point Optical Fibre Sensor for Proton Therapy
Authors: Penner Crystal , Usherovich Samuel , Andru Sophia , Bélanger-Champagne Camille , Duzenli Cheryl , Stoeber Boris , Hoehr Cornelia
Published: March 2024

As the technology to deliver precise and very high radiotherapeutic doses with narrow margins grows to better serve patients with complex radiotherapeutic needs, so does the need for sensors and sensor systems that can reliably deliver multi-point dose monitoring and dosimetry for enhanced safety and access. To address this need, we investigated a novel five-point scintillator system for simultaneously sampling points across a 74 MeV proton beam with a Hamamatsu 16-channel MPPC array. We studied the response across beam widths from 25 mm down to 5 mm in diameter and in multiple depths to observe beam penumbrae and output factors as well as depth–dose. We found through comparison to ionization chambers and radiochromic film that the array is capable of measurements accurate to within 8% in the centre of proton beams from 5 to 25 mm in diameter, and within 2% at 3.5 cm depth in water. The results from three trials are repeatable after calibration to within <1%. Overall, the five optical fibre sensor system shows promise as a fast, multipoint relative dosimetry system.
CSIM: A Fast Community Detection Algorithm Based on Structure Information Maximization
Authors: Liu Yiwei , Liu Wencong , Tang Xiangyun , Yin Hao , Yin Peng , Xu Xin , Wang Yanbin
Published: March 2024

Community detection has been a subject of extensive research due to its broad applications across social media, computer science, biology, and complex systems. Modularity stands out as a predominant metric guiding community detection, with numerous algorithms aimed at maximizing modularity. However, modularity encounters a resolution limit problem when identifying small community structures. To tackle this challenge, this paper presents a novel approach by defining community structure information from the perspective of encoding edge information. This pioneering definition lays the foundation for the proposed fast community detection algorithm CSIM, boasting an average time complexity of only O(nlogn). Experimental results showcase that communities identified via the CSIM algorithm across various graph data types closely resemble ground truth community structures compared to those revealed via modularity-based algorithms. Furthermore, CSIM not only boasts lower time complexity than greedy algorithms optimizing community structure information but also achieves superior optimization results. Notably, in cyclic network graphs, CSIM surpasses modularity-based algorithms in effectively addressing the resolution limit problem.
An Energy Consumption Model for SRAM-Based In-Memory-Computing Architectures
Authors: Akgül Berke , Karalar Tufan Coşkun
Published: March 2024

In this paper, a mathematical model for obtaining energy consumption of IMC architectures is constructed. This model provides energy estimation based on the distribution of a specific dataset. In addition, the estimation reduces the required simulation time to create an energy consumption model of SRAM-based IMC architectures. To validate our model with realistic data, the energy consumption of IMC is compared by using NeuroSim V3.0 for the CIFAR-10 and MNIST-like datasets. Furthermore, an application is created with our model to select highest performing quantization mapping based upon the parameters of energy consumption and accuracy.
A Non-Destructive Detection and Grading Method of the Internal Quality of Preserved Eggs Based on an Improved ConvNext
Authors: Tang Wenquan , Zhang Hao , Chen Haoran , Fan Wei , Wang Qiaohua
Published: March 2024

As a traditional delicacy in China, preserved eggs inevitably experience instances of substandard quality during the production process. Chinese preserved egg production facilities can only rely on experienced workers to select the preserved eggs. However, the manual selection of preserved eggs presents challenges such as a low efficiency, subjective judgments, high costs, and hindered industrial production processes. In response to these challenges, this study procured the transmitted imagery of preserved eggs and refined the ConvNeXt network across four pivotal dimensions: the dimensionality reduction of model feature maps, the integration of multi-scale feature fusion (MSFF), the incorporation of a global attention mechanism (GAM) module, and the amalgamation of the cross-entropy loss function with focal loss. The resultant refined model, ConvNeXt_PEgg, attained proficiency in classifying and grading preserved eggs. Notably, the improved model achieved a classification accuracy of 92.6% across the five categories of preserved eggs, with a grading accuracy of 95.9% spanning three levels. Moreover, in contrast to its predecessor, the refined model witnessed a 24.5% reduction in the parameter volume, alongside a 3.2 percentage point augmentation in the classification accuracy and a 2.8 percentage point boost in the grading accuracy. Through meticulous comparative analysis, each enhancement exhibited varying degrees of performance elevation. Evidently, the refined model outshone a plethora of classical models, underscoring its efficacy in discerning the internal quality of preserved eggs. With its potential for real-world implementation, this technology portends to heighten the economic viability of manufacturing facilities.
Long-Term Tissue Preservation at Ambient Temperature for Post-Mass Fatality Incident DNA-Based Victim Identification
Authors: Chan Xavier Liang Shun , Lai Shumei Michelle , bin Hamdan Danial Asyraaf , Ng Yee Bin , Yim Onn Siong , Syn Christopher Kiu Choong
Published: March 2024

In a mass fatality incident (MFI), effective preservation of tissue samples is the cornerstone for downstream DNA-based identification of victims. This is commonly achieved through freezing of tissue samples excised from bodies/fragmented remains which may be buried or stored in refrigerated containers. This may, however, not be possible depending on the nature of the MFI; in particular, during armed conflict/war where extended periods of electrical outages would be expected. The present study compared the effectiveness of long-term tissue preservation at ambient temperatures using two commercial products (non-iodized kitchen salt and a 40% alcoholic beverage) against a chemical preservative (Allprotect™ Tissue Reagent (Qiagen, Germantown, MD, USA)) and freezing at −20 °C. Bovine muscle tissue, used as a proxy for human tissue, was treated with the four preservation methods and sampled at six different time-points over a 24-month period. All four methods were able to preserve the bovine tissue, generally yielding STR-PCR (Short Tandem Repeat-Polymerase Chain Reaction) amplicons > 200 bp in size even at the end of 24 months. Gel electrophoresis, however, indicated that salt was more effective in preserving DNA integrity with high-molecular-weight DNA clearly visible as compared to the low-molecular-weight DNA smears observed in the other methods. This study also proposes a simple process for the rapid and low-cost preservation of tissue samples for long-term storage at ambient temperatures in support of post-incident victim identification efforts.
Beyond the Anticodon: tRNA Core Modifications and Their Impact on Structure, Translation and Stress Adaptation
Authors: Yared Marcel-Joseph , Marcelot Agathe , Barraud Pierre
Published: March 2024

Transfer RNAs (tRNAs) are heavily decorated with post-transcriptional chemical modifications. Approximately 100 different modifications have been identified in tRNAs, and each tRNA typically contains 5–15 modifications that are incorporated at specific sites along the tRNA sequence. These modifications may be classified into two groups according to their position in the three-dimensional tRNA structure, i.e., modifications in the tRNA core and modifications in the anticodon-loop (ACL) region. Since many modified nucleotides in the tRNA core are involved in the formation of tertiary interactions implicated in tRNA folding, these modifications are key to tRNA stability and resistance to RNA decay pathways. In comparison to the extensively studied ACL modifications, tRNA core modifications have generally received less attention, although they have been shown to play important roles beyond tRNA stability. Here, we review and place in perspective selected data on tRNA core modifications. We present their impact on tRNA structure and stability and report how these changes manifest themselves at the functional level in translation, fitness and stress adaptation.
On Segurasaurus (Squamata: Pythonomorpha), a New Genus of Lizard from the Cenomanian (Upper Cretaceous) of Portugal
Authors: Berrocal-Casero Mélani , Pimentel Ricardo , Callapez Pedro Miguel , Barroso-Barcenilla Fernando , Ozkaya de Juanas Senay
Published: March 2024

Carentonosaurus soaresi was recently described in the uppermost middle Cenomanian (Upper Cretaceous) of Casais dos Carecos (Coimbra, western Portugal) based on a diverse set of new material (cervical and dorsal vertebrae) of the Pythonomorpha lizard. The main morphological characteristics observed in the vertebrae used for the diagnosis of this species are the presence of distinct lateral and subcentral foramina, highly laterally projected paradiapophyses beyond the prezygapophyses, a low subrectangular neural spine ornamented with longitudinal grooves, and dorsal vertebrae displaying a sagittal furrow along the ventral surface. Additional diagnostic details observed both in new material and the previously studied vertebrae are described herein, such as the presence of keels in the zygantrum and zygosphene. These and other important morphological characteristics present in the species soaresi are absent in the genotype Carentonosaurus mineaui and in other known Squamata, allowing for the definition of the new genus Segurasaurus.
Potato Biofortification: A Systematic Literature Review on Biotechnological Innovations of Potato for Enhanced Nutrition
Authors: Agrawal Smita , Kumar Amit , Gupta Yash , Trivedi Ayushi
Published: March 2024

Potato biofortification is a comprehensive approach aimed at enhancing the nutritional content of potatoes, addressing widespread nutrient deficiencies and contributing to global food security. This systematic review examines the existing literature on various aspects of potato biofortification, encompassing genetic, agronomic, and biotechnological strategies. The review highlights the nutritional significance of potatoes, emphasizing their role as a staple food in many regions. Genetic approaches to biofortification involve the identification and use of natural variations in potato germplasm to develop varieties with elevated levels of essential nutrients. This includes targeting key micronutrients, such as iron, zinc, and vitamins, through traditional breeding methods. The review explores the genetic diversity within potato germplasm and the potential for breeding programs to develop nutrient-rich varieties. Agronomic practices play a crucial role in potato biofortification, with studies demonstrating the impact of tuber priming and the application of mineral fertilizers on nutrient concentrations in potatoes. The review delves into the intricacies of agronomic biofortification, emphasizing the importance of precise dosages and timing for optimal results. Biotechnological tools, including transgenic and non-transgenic approaches, are discussed in the context of potato biofortification. The review evaluates the efficiency and ethical considerations associated with the development of biofortified transgenic potatoes and emphasizes the significance of non-transgenic approaches in addressing consumer concerns and regulatory barriers. Overall, this systematic review provides a comprehensive overview of the current state of potato biofortification research. It synthesizes findings from diverse studies, offering insights into the potential of biofortified potatoes to address hidden hunger and contribute to improved nutritional outcomes. This review also identifies knowledge gaps and areas for future research, guiding the direction of efforts to harness the full potential of potato biofortification for global food and nutrition security.
Whipple Disease Presenting as Isolated Transverse Myelitis with Permanent Neurological Damage in a Patient with Systemic Lupus Erythematosus: A Case Report of a Difficult Diagnosis with a Literature Review
Authors: Saffioti Carolina , Nebiolo Marta , Caorsi Roberta , Mesini Alessio , Severino Mariasavina , Brisca Giacomo , Castagnola Elio , Gattorno Marco
Published: March 2024

We describe an atypical case of Whipple disease exclusively involving the spinal cord in an adolescent receiving immunosuppressive therapy for systemic lupus erythematosus. The diagnosis was particularly difficult since lupus and Whipple disease can present similar clinical features and the patient’s prolonged contact with sewage was initially not mentioned. A literature review of the clinical, imaging, diagnostic, and therapeutic challenges of Whipple disease is also performed.
Coaching Home Care Clients to Prepare Their Homes for Safe Care Visits: A Mixed-Methods Study to Evaluate a Nurse-Led Educational Intervention Process
Authors: Markkanen Pia K. , Gore Rebecca J. , Sama Susan R. , Lindberg John E. , Galligan Catherine J. , Quinn Margaret M.
Published: March 2024

Assuring home care (HC) workers’ safety is challenging because the work environment is a private home. This paper presents the process evaluation for a proof-of-concept safety intervention study to assess whether nurse-led safety coaching, using motivational interviewing and a safety handbook, could enable HC clients to improve safety in their homes. The process evaluation objectives were to (i) document the intervention’s implementation progress and (ii) assess the intervention’s dose delivery, dose reception, and fidelity. Five agencies employing liaisons (n = 5) and nurse managers (NMs, n = 8) implemented this study’s intervention and control arms. NMs assigned to the intervention arm (n = 6) coached 34 clients. Process evaluation metrics were assessed with mixed-methods data from (i) surveys completed by NMs during the intervention, (ii) postintervention audio-recorded and transcribed interviews (n = 6) with NMs and liaisons, and (iii) study progress tracking tools. The delivered dose efficiency was 85%, measured by the distribution of safety handbook copies to clients. About 94% of clients (n = 32) were considered “engaged” or “maybe engaged” during the safety coaching. Most coached clients (n = 30) were reachable for follow-up by NMs to assess intervention progress. Despite challenges, the intervention was implemented with good fidelity. Safety coaching can be applied in many HC contexts in larger populations.
Chemical Constituents of Stinging Nettle (Urtica dioica L.): A Comprehensive Review on Phenolic and Polyphenolic Compounds and Their Bioactivity
Authors: Đurović Saša , Kojić Ivan , Radić Danka , Smyatskaya Yulia A. , Bazarnova Julia G. , Filip Snežana , Tosti Tomislav
Published: March 2024

Polyphenolic compounds are of great interest in today’s science. Naturally, they occur in plants and other sources in many different forms. Their wide range of biological activity has attracted the attention of the scientific community. One of the sources of phenolic compounds is stinging nettle (Urtica dioica L.), a common plant in almost all parts of the world. A long tradition of utilization and an interesting chemical profile make this plant a fascinating and extensive object of study. The chemical profile also allows this plant to be used as a food and a pigment source in the food, pharmaceutical, and cosmetic industries. Previously conducted studies found phenolic acids and polyphenolic compounds in root, stalk, and stinging nettle leaves. Different extraction techniques were usually used to isolate them from the leaves. Obtained extracts were used to investigate biological activity further or formulate different functional food products. This study aimed to collect all available knowledge about this plant, its chemical composition, and biological activity and to summarize this knowledge with particular attention to polyphenolic compounds and the activity and mechanisms of their actions.
Genome-Wide Identification of the 14-3-3 Gene Family and Its Involvement in Salt Stress Response through Interaction with NsVP1 in Nitraria sibirica Pall
Authors: Wan Xihong , Duan Rongfeng , Zhang Huaxin , Zhu Jianfeng , Wu Haiwen , Zhang Huilong , Yang Xiuyan
Published: March 2024

14-3-3 proteins are widely distributed in eukaryotic cells and play an important role in plant growth, development, and stress tolerance. This study revealed nine 14-3-3 genes from the genome of Nitraria sibirica Pall., a halophyte with strong salt tolerance. The physicochemical properties, multiple sequence alignment, gene structure and motif analysis, and chromosomal distributions were analyzed, and phylogenetic analysis, cis-regulatory elements analysis, and gene transcription and expression analysis of Ns14-3-3s were conducted. The results revealed that the Ns14-3-3 gene family consists of nine members, which are divided into two groups: ε (four members) and non-ε (five members). These members are acidic hydrophilic proteins. The genes are distributed randomly on chromosomes, and the number of introns varies widely among the two groups. However, all genes have similar conserved domains and three-dimensional protein structures. The main differences are found at the N-terminus and C-terminus. The promoter region of Ns14-3-3s contains multiple cis-acting elements related to light, plant hormones, and abiotic stress responses. Transcriptional profiling and gene expression pattern analysis revealed that Ns14-3-3s were expressed in all tissues, although with varying patterns. Under salt stress conditions, Ns14-3-3 1a, Ns14-3-3 1b, Ns14-3-3 5a, and Ns14-3-3 7a showed significant changes in gene expression. Ns14-3-3 1a expression decreased in all tissues, Ns14-3-3 7a expression decreased by 60% to 71% in roots, and Ns14-3-3 1b expression increased by 209% to 251% in stems. The most significant change was observed in Ns14-3-3 5a, with its expression in stems increasing by 213% to 681%. The yeast two-hybrid experiments demonstrated that Ns14-3-3 5a interacts with NsVP1 (vacuolar H+-pyrophosphatase). This result indicates that Ns14-3-3 5a may respond to salt stress by promoting ionic vacuole compartmentalization in stems and leaves through interactions with NsVP1. In addition, N. sibirica has a high number of stems, allowing it to compartmentalize more ions through its stem and leaf. This may be a contributing factor to its superior salt tolerance compared to other plants.