bluuebunny/crossref_metadata_2025 huggingface dataset:
Systematic review and meta-analysis: evaluating response to empiric anti-TNF dose intensification for secondary loss of response in Crohn’s disease
Authors: Srinivasan Ashish , Gilmore Robert , van Langenberg Daniel , De Cruz Peter
Published: February 2022

Introduction:

Anti-tumor necrosis factor (TNF) dose intensification represents an effective method of overcoming secondary loss of response (LOR); however, a subset of patients may not respond (tertiary non-response), or fail to demonstrate durable response (tertiary LOR) to intensified dosing. This systematic review and meta-analysis aimed to evaluate these outcomes to determine the clinical effectiveness of empiric dose intensification in Crohn’s disease.

Methods:

Multiple databases including MEDLINE and EMBASE were interrogated to identify studies that reported outcomes following anti-TNF dose intensification to address secondary LOR in Crohn’s disease. Studies that used anti-TNF levels as the primary basis for dose intensification were excluded. Studies that reported (1) tertiary response and tertiary non-response within 6 months or (2) tertiary response and tertiary LOR beyond 6 months, were pooled using a random effects model with risk ratio (RR) derived, quantifying the effect of each comparison.

Results:

Twenty-six studies reported outcomes following anti-TNF dose intensification to address secondary LOR. Short-term response within 12 weeks of any dose-intensification strategy was 33–90%, while sustained response (⩾48 weeks) was achieved in 25–85%. Tertiary non-response occurred in up to 45% of intensified patients within 6 months of anti-TNF dose intensification, while tertiary LOR beyond 6 months occurred in up to 64% of patients. Tertiary response was more likely than tertiary non-response within 6 months (RR 2.58, 95% CI (1.76, 3.79), I2 = 82%, 12 studies), while sustained response beyond 6 months compared to tertiary LOR (RR 1.10 (0.75, 1.61) I2 = 85%, 7 studies) was less convincing.

Conclusion:

Although anti-TNF dose intensification is clinically effective in patients with Crohn’s disease, particularly within the first 6 months, a proportion of patients will fail to demonstrate short-term and/or sustained clinical response. Hence, clinical reassessment following anti-TNF dose intensification, particularly beyond 6 months, remains important to differentiate between effective and ineffective dose-intensification strategies.
Demystifying the academy: Resistance, ethics and abuse of power
Authors: Bravo-Moreno (PhD) Ana
Published: February 2022

The purpose of this article is to examine academia and the abuse of power based on auto-ethnographic research. I draw on my experiences across 12 universities in different locations in Spain, the UK and the USA that expose the way power is embedded in institutions of higher education and how it is maintained. This article analyses the exploration of inequalities which concerns particular social divisions, for example, gender, social class, ethnicity, non-national status and the intersection of these categories in particular sociocultural and historical contexts where I conducted my studies, research and teaching for more than 30 years. Employing auto-ethnography has allowed me to examine multi-layered lived experiences in the three countries intertwined with axes of inequality. Thanks to the dual focus on individual experiences and social contexts, this article shows how different systems of domination have shaped my experiences as a student and as a member of faculty in a transnational context. This heuristic approach has challenged me to generate meaning within a framework of ethics and social justice, recognizing that academia often excludes and marginalizes. Thus, this qualitative research enables marginal voices and the articulation of silenced narratives, hence expanding our knowledge of the relationship between power and academia.
Disseminated gonococcal infection of the shoulder mimicking rheumatoid arthritis
Authors: Kobayashi Shunsuke , Izaki Teruaki , Miyake Satoshi , Arashiro Yasuhara , Yamamoto Takuaki
Published: February 2022

Introduction

Gonococcal arthritis is a characteristic of disseminated gonococcal infection (DGI). DGI arthritis is one of the most serious orthopedic emergencies because it can result in rapidly progressive joint damage, but it is often difficult to diagnose. Delayed treatment can result in the development of osteomyelitis in the adjacent bone, similar to other types of bacterial arthritis.

Method

We report a case of gonococcal osteomyelitis associated with DGI that was initially treated as rheumatoid arthritis. The diagnosis was confirmed by DNA testing of synovium collected during arthroscopic debridement.

Results

Seven years after the initial consultation, there was no acute-phase reactant, the arthritic changes had improved over time and the range of motion had increased. DGI may be difficult to confirm, but it is one of the most important entities that should be differentiated in the treatment of arthritis.

Conclusion

Clinicians need to keep in mind that blood and synovial fluid cultures often do not lead to a definitive diagnosis.
Millennials’ Self-Identity and Intention to Purchase Sustainable Products
Authors: Mishra Sita , Shukla Yupal , Malhotra Gunjan , Chatterjee Ravi , Rana Jyoti
Published: February 2022

With the rising challenges related to social, economic, and climatic factors, the focus toward adoption of sustainable products is rising. Increasing focus on sustainable products, has influenced the consumer shopping behavior toward greener products. The consumers’ participation showntoward sustainable products is what defines their behavior toward its purchase. Understanding of sustainable consumption in emerging markets is still unexplored which calls for further research. A structured questionnaire was used to collect data from the millennials who belong to the working population of India and have awareness regarding sustainable products and its impact on the environment. The results show that there is a direct relationship between environment self-identity (ESI) and the intention to purchase a sustainable product (PI). Psychological ownership (PO) also plays a role of a partial mediator for the relationship between SI and PI. Environment Concern (EC) and social influence (Soc) are critical factors; their relationship also matters to comprehend the results concretely
Synthesis and Antitumor Activity of Evodiamine Derivatives With Nitro, Amino, and Methoxy Groups
Authors: Yang Ruolan , Ma Jingjing , Guo Hui , Meng Qinghua , Wang Yuwei , Yan Hao , Jin Ruyi , Li Zhi , Meng Lingjie
Published: February 2022

MS, and IR, 1H NMR and 13C NMR spectroscopy were employed to elucidate 4 novel evodiamine (EVO) derivatives with nitro, amino, and methoxy groups, namely 2-NO2-EVO (7a), 10-OCH3-2-NO2-EVO (7b), 2-NH2-EVO (8a), and 10-OCH3-2-NH2-EVO (8b). The amino compounds (8a, 8b) were obtained by the reduction of nitro derivatives (7a, 7b) with SnCl2/HCl. The antiproliferative activities of these compounds were tested by Cell Counting Kit-8 assay for 48 h against the MDA-MB-231 and sw620 cancer cell lines, as well as the normal LO2 cells. The in vitro experiment showed that 8a possesses the most potent inhibitory activities against MDA-MB-231 and SW620 cells, with IC50 values of 0.79 and 1.28 μM, respectively. The cytotoxicity of 8a against the 2 cancer cell lines was higher than that of EVO.
Prediction the Molecular Mechanism of Shengmai Injection in Acute Treatment of COVID-19 Based on Network Pharmacology
Authors: Wang Chen , Liu Ao-lei , Wu He-zhen , Yang Yan-fang
Published: February 2022

Objective: To predict the mechanism of Shengmai Injection (SMI) in the acute treatment of COVID-19 by network pharmacology and molecular docking. Methods: Search the compounds in the Traditional Chinese Medicine Systems Pharmacology (TCMSP), and screen them by Drug-like properties (DL) and Oral bioavailability (OB); Using PharmMapper database and GeneCards database to collect compounds targets and COVID-19 targets, and using UniProt database to standardize the names of target genes; Using DAVID database for KEGG pathway annotation and GO bioinformatics analysis; Using Cytoscape 3.8.2 software and STRING 10.5 database to construct “Component-Target-Pathway” network and Protein-Protein Interaction network (PPI); Using molecular docking to predict the binding ability of key compounds and key proteins. Results: A total of 34 active components, 38 core targets and 180 signaling pathways were screened out. The results of molecular docking showed that Schisantherin A and Moupinamide have strong binding with EGFR and MAPK1. Conclusion: The key active compounds of SMI in the treatment of COVID-19 may be Schisantherin A and Moupinamide, and the molecular mechanism may be related to key targets such as EGFR and MAPK1, and may be involved in the PI3K-Akt signaling pathway and MAPK signaling pathway.
Total Synthesis and Anti-Inflammatory Activity of Velutone F
Authors: Li Ying , Wu Min , Dong Hongbo , Yu Pei , Lu Lan , Du Weihong , Cao Shenghua
Published: February 2022

Velutone F (1), a natural bioactive chalcone isolated from Millettia velutina Dunn, possesses significant anti-inflammatory activity. In this study, we have accomplished the total synthesis of velutone F (1), along with its analogues 2 and 3, from a common starting material cyclohexandione in 5 to 7 steps. The anti-inflammatory activities of compounds 1 to 3 were determined against nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and all of them exhibited different levels of anti-inflammatory activity.
Internal Medicine Resident Adherence to Evidence-Based Practices in Management of Diabetes Mellitus
Authors: Mehta Chirag , Cohen David , Jaisinghani Priya , Parikh Payal
Published: February 2022

Objective

Diabetic preventative health maintenance involves (1) adherence to guidelines and (2) appropriate documentation. This study evaluates the frequency with which internal medicine (IM) residents met these two outcomes. We also evaluated if there were differences in outcomes between resident classes.

Methods

In this retrospective analysis, 234 diabetic outpatient visits were analyzed. Guidelines were derived from the American Diabetes Association (ADA). The outcomes for each guideline were averaged and stratified by resident class. Averages within and between classes were compared using the student's paired t-test and one-way ANOVA respectively.

Results

Classes were most adherent to A1c testing guidelines (PGY-1 96.1%, PGY-2 97.6%, and PGY-3 95.9%). PGY-1 and PGY-2 classes were least adherent to podiatry (27.5% and 17.6% respectively), whereas PGY-3 had equal least adherence rates to podiatry and lifestyle modification guidelines (36.7%). All classes had highest rates in documenting A1c findings (PGY-1 92.2%, PGY-2 97.6%, and PGY-3 85.7%) and lowest rates in documenting relevant podiatry information (PGY-1 5.9%, PGY-2 5.9%, and PGY-3 11.2%). Comparing sequential resident classes, there was a decline in lifestyle counselling and documentation from PGY-1 to PGY-2. From PGY-2 to PGY-3, there was improvement in adherence to statin, podiatry, microalbuminuria, and monofilament guidelines. There was also improvement in documenting statin and monofilament usage, however, A1c reporting declined.

Conclusion

The findings of the study suggest disproportionate levels of care in diabetes preventative management. Additionally, program directors should take caution in assuming linear improvement with sequential resident classes.
Using mobile phone big data to discover the spatial patterns of rural migrant workers’ return to work in China’s three urban agglomerations in the post-COVID-19 era
Authors: Liu Kai , Zhao Pengjun , Wan Dan , Hai Xiaodong , He Zhangyuan , Liu Qiyang , Qu Yonghui , Zhang Xue , Li Kaixi , Yu Ling
Published: February 2022

Knowing how workers return to work is a key policymaking issue for economic recovery in the post-COVID-19 era. This paper uses country-wide time-series mobile phone big data (comparing monthly and annual figures), obtained between February 2019 and October 2019 and between February 2020 and October 2020, to discover the spatial patterns of rural migrant workers’ (RMWs’) return to work in China’s three urban agglomerations (UAs): the Beijing–Tianjin–Hebei Region, the Yangtze River Delta and the Pearl River Delta. Spatial patterns of RMWs’ return to work and how these patterns vary with location, city level and human attribute were investigated using the fine-scale social sensing related to post-pandemic human mobility. The results confirmed the multidimensional spatiotemporal differentiations, interaction effects between variable pairs and effects of the actual situation on the changing patterns of RMWs’ return to work. The spatial patterns of RMWs’ return to work in China’s major three UAs can be regarded as a comprehensive and complex interaction result accompanying the nationwide population redistribution, which was affected by various hidden factors. Our findings provide crucial implications and suggestions for data-informed policy decisions for a harmonious society in the post-COVID-19 era.
Live Cycle Delight: An Urban Fitness Boutique Fights to Stay Alive
Authors: Scott Crystal J.
Published: February 2022

Live Cycle Delight (LCD) and Live Cycle Delight Hot (LCD Hot) is a boutique fitness studio founded in 2017 in Detroit, Michigan, by owner, Amina Daniels. The studios are nestled in a quaint up and coming historic multicultural neighborhood just outside of Downtown Detroit. After winning two start-up contests in 2015, Amina’s business started turning a profit in year two. LCD had a loyal client following and Amina was hopeful that LCD would continue growing and expanding. In March 2020, all of that changed when LCD, like all other gyms in Michigan, had to close their doors. In addition to the pandemic, there was social unrest across many states in the country and Amina’s outward support for Black Lives Matter (BLM) did not sit well with some of her customers. Now 9 months later, Amina is wondering how to survive. Many customers have become accustomed to virtual and online fitness classes and some have invested in at-home gyms and fitness equipment. Amina has considered adding on-demand classes, offering more virtual offerings and developing partnerships with other gyms across the country. In the meantime, she has lost 80% of her customers and must figure out how to rebuild.
SampleSizePlanner: A Tool to Estimate and Justify Sample Size for Two-Group Studies
Authors: Kovacs Marton , van Ravenzwaaij Don , Hoekstra Rink , Aczel Balazs
Published: February 2022

Planning sample size often requires researchers to identify a statistical technique and to make several choices during their calculations. Currently, there is a lack of clear guidelines for researchers to find and use the applicable procedure. In the present tutorial, we introduce a web app and R package that offer nine different procedures to determine and justify the sample size for independent two-group study designs. The application highlights the most important decision points for each procedure and suggests example justifications for them. The resulting sample-size report can serve as a template for preregistrations and manuscripts.
An uncommon unintentionally retained foreign object (URFO): The retained surgical specimen
Authors: Simioni Andrea , Fransman Ryan , Safar Bashar , Haut Elliott R , Atallah Chady
Published: February 2022

Unintentionally Retained Foreign Objects (URFO) are an uncommon, but significant type of patient harm. Retained surgical specimens are new entities that accompanied the surge of minimally invasive surgery (MIS). Despite being rare sentinel events, they are associated with increased morbidity, healthcare cost, and liability. We present a case of a retained surgical specimen, identified after surgical closure but before patient extubation, thanks to routine utilization of end-of-procedure checklists.
Fetal vs adult megakaryopoiesis
Authors: Davenport Patricia , Liu Zhi-Jian , Sola-Visner Martha
Published: February 2022

Fetal and neonatal megakaryocyte progenitors are hyperproliferative compared with adult progenitors and generate a large number of small, low-ploidy megakaryocytes. Historically, these developmental differences have been interpreted as “immaturity.” However, more recent studies have demonstrated that the small, low-ploidy fetal and neonatal megakaryocytes have all the characteristics of adult polyploid megakaryocytes, including the presence of granules, a well-developed demarcation membrane system, and proplatelet formation. Thus, rather than immaturity, the features of fetal and neonatal megakaryopoiesis reflect a developmentally unique uncoupling of proliferation, polyploidization, and cytoplasmic maturation, which allows fetuses and neonates to populate their rapidly expanding bone marrow and blood volume. At the molecular level, the features of fetal and neonatal megakaryopoiesis are the result of a complex interplay of developmentally regulated pathways and environmental signals from the different hematopoietic niches. Over the past few years, studies have challenged traditional paradigms about the origin of the megakaryocyte lineage in both fetal and adult life, and the application of single-cell RNA sequencing has led to a better characterization of embryonic, fetal, and adult megakaryocytes. In particular, a growing body of data suggests that at all stages of development, the various functions of megakaryocytes are not fulfilled by the megakaryocyte population as a whole, but rather by distinct megakaryocyte subpopulations with dedicated roles. Finally, recent studies have provided novel insights into the mechanisms underlying developmental disorders of megakaryopoiesis, which either uniquely affect fetuses and neonates or have different clinical presentations in neonatal compared with adult life.
PIM2 kinase has a pivotal role in plasmablast generation and plasma cell survival, opening up novel treatment options in myeloma
Authors: Haas Marion , Caron Gersende , Chatonnet Fabrice , Manenti Stéphane , Alaterre Elina , Devin Julie , Delaloy Céline , Bertolin Giulia , Viel Roselyne , Pignarre Amandine , Llamas-Gutierrez Francisco , Marchalot Anne , Decaux Olivier , Tarte Karin , Delpy Laurent , Moreaux Jérôme , Fest Thierry
Published: February 2022

The differentiation of B cells into plasmablasts (PBs) and then plasma cells (PCs) is associated with extensive cell reprogramming and new cell functions. By using specific inhibition strategies (including a novel morpholino RNA antisense approach), we found that early, sustained upregulation of the proviral integrations of Moloney virus 2 (PIM2) kinase is a pivotal event during human B-cell in vitro differentiation and then continues in mature normal and malignant PCs in the bone marrow. In particular, PIM2 sustained the G1/S transition by acting on CDC25A and p27Kip1 and hindering caspase 3–driven apoptosis through BAD phosphorylation and cytoplasmic stabilization of p21Cip1. In PCs, interleukin-6 triggered PIM2 expression, resulting in antiapoptotic effects on which malignant PCs were particularly dependent. In multiple myeloma, pan-PIM and myeloid cell leukemia-1 (MCL1) inhibitors displayed synergistic activity. Our results highlight a cell-autonomous function that links kinase activity to the newly acquired secretion ability of the PBs and the adaptability observed in both normal and malignant PCs. These findings should finally prompt the reconsideration of PIM2 as a therapeutic target in multiple myeloma.
Molecular characterization of mutant TP53 acute myeloid leukemia and high-risk myelodysplastic syndrome
Authors: Grob Tim , Al Hinai Adil S. A. , Sanders  Mathijs A. , Kavelaars François G. , Rijken Melissa , Gradowska Patrycja L. , Biemond Bart J. , Breems Dimitri A. , Maertens Johan , van Marwijk Kooy Marinus , Pabst Thomas , de Weerdt Okke , Ossenkoppele Gert J. , van de Loosdrecht Arjan A. , Huls Gerwin A. , Cornelissen Jan J. , Beverloo H. Berna , Löwenberg Bob , Jongen-Lavrencic Mojca , Valk Peter J. M.
Published: February 2022

Substantial heterogeneity within mutant TP53 acute myeloid leukemia (AML) and myelodysplastic syndrome with excess of blast (MDS-EB) precludes the exact assessment of prognostic impact for individual patients. We performed in-depth clinical and molecular analysis of mutant TP53 AML and MDS-EB to dissect the molecular characteristics in detail and determine its impact on survival. We performed next-generation sequencing on 2200 AML/MDS-EB specimens and assessed the TP53 mutant allelic status (mono- or bi-allelic), the number of TP53 mutations, mutant TP53 clone size, concurrent mutations, cytogenetics, and mutant TP53 molecular minimal residual disease and studied the associations of these characteristics with overall survival. TP53 mutations were detected in 230 (10.5%) patients with AML/MDS-EB with a median variant allele frequency of 47%. Bi-allelic mutant TP53 status was observed in 174 (76%) patients. Multiple TP53 mutations were found in 49 (21%) patients. Concurrent mutations were detected in 113 (49%) patients. No significant difference in any of the aforementioned molecular characteristics of mutant TP53 was detected between AML and MDS-EB. Patients with mutant TP53 have a poor outcome (2-year overall survival, 12.8%); however, no survival difference between AML and MDS-EB was observed. Importantly, none of the molecular characteristics were significantly associated with survival in mutant TP53 AML/MDS-EB. In most patients, TP53 mutations remained detectable in complete remission by deep sequencing (73%). Detection of residual mutant TP53 was not associated with survival. Mutant TP53 AML and MDS-EB do not differ with respect to molecular characteristics and survival. Therefore, mutant TP53 AML/MDS-EB should be considered a distinct molecular disease entity.
IKK-mediated CYLD phosphorylation and cellular redox activity
Authors: Erol Adnan
Published: February 2022

Oxidative stress is important in the development of obesity-related nephropathy (ORN). A causal relationship between IKK and ORN via CYLD-mediated inhibition of NRF2 has been described. However, contradictory explanations about the functioning of the mechanisms that will be effective in the pathogenesis require clarification.
Slc25a5 regulates adipogenesis by modulating ERK signaling in OP9 cells
Authors: Zhu Shenglong , Wang Wei , Zhang Jingwei , Ji Siyu , Jing Zhe , Chen Yong Q.
Published: February 2022

Background

A comprehensive understanding of the molecular mechanisms of adipogenesis is a critically important strategy for identifying new targets for obesity intervention.

Methods

Transcriptomic and lipidomic approaches were used to explore the functional genes regulating adipogenic differentiation and their potential mechanism of action in OP9 cells and adipose-derived stem cells. Oil Red O staining was used to detect oil droplets in adipocytes.

Results

RNA sequencing (RNA-seq) showed that Slc25a5 expression was significantly upregulated in adipogenic differentiation. Depletion of Slc25a5 led to the suppressed expression of adipogenesis-related genes, reduced the accumulation of triglycerides, and inhibited PPARγ protein expression. Moreover, the knockdown of Slc25a5 resulted in significant reduction of oxidative phosphorylation (OXPHOS) protein expression (ATP5A1, CQCRC2, and MTCO1) and ATP production. The RNA-seq and real-time quantitative polymerase chain reaction (RT–qPCR) results suggested that adipogenic differentiation is possibly mediated by ERK1/2 phosphorylation, and this hypothesis was confirmed by intervention with PD98059 (an ERK 1/2 inhibitor).

Conclusions

This study indicates that Slc25a5 inhibits adipogenesis and might be a new therapeutic target for the treatment of obesity.
ERp57/PDIA3: new insight
Authors: Chichiarelli Silvia , Altieri Fabio , Paglia Giuliano , Rubini Elisabetta , Minacori Marco , Eufemi Margherita
Published: February 2022

The ERp57/PDIA3 protein is a pleiotropic member of the PDIs family and, although predominantly located in the endoplasmic reticulum (ER), has indeed been found in other cellular compartments, such as the nucleus or the cell membrane. ERp57/PDIA3 is an important research target considering it can be found in various subcellular locations. This protein is involved in many different physiological and pathological processes, and our review describes new data on its functions and summarizes some ligands identified as PDIA3-specific inhibitors.
Analysis of autosomal dominant genes impacted by copy number loss in 24,844 fetuses without structural abnormalities
Authors: Chen Lin , Wang Li , Yin Daishu , Tang Feng , Zeng Yang , Zhu Hongmei , Wang Jing
Published: February 2022

Background

The broad application of high-resolution chromosome detection technology in prenatal diagnosis has identified copy number loss (CNL) involving autosomal dominant (AD) genes in certain fetuses. Exon sequencing of fetuses exhibiting structural anomalies yields diagnostic information in up to 20% of cases. However, there is currently no relevant literature about the genetic origin and pregnancy outcome of CNL involving AD genes in fetuses without structural abnormalities.

Results

This was a prospective study involving pregnant women who underwent amniocentesis for fetal copy number variation sequencing (CNVseq). Detection of parent-of-origin was suggested in cases of samples with CNL involving AD genes and the pregnancy outcome was monitored. Amniotic fluid samples from 24,844 fetuses without structural abnormalities were successfully tested via CNVseq. The results showed that 134 fetuses (0.5%) had small CNL (< 10 Mb) containing AD genes, after excluding microdeletion and microduplication syndrome and polymorphisms. By monitoring the pregnancy outcomes of the 134 fetuses, we found that 104 (77.6%) were good, 13 (9.7%) were adverse, and 17 (12.7%) pregnant women voluntarily chose to terminate pregnancy. Of the 13 fetuses with adverse pregnancy outcomes, only 2 fetuses had phenotypes consistent with those of diseases caused by AD genes involved in CNL.

Conclusions

The overall prognosis for fetuses without family history or structural abnormalities but with small CNL containing AD genes detected during pregnancy is good. The genetic origin, overlap status of established haploinsufficient gene and/or region, size of the CNL, and genetic mode may affect the pathogenicity of the CNL.
Soil pH: a key edaphic factor regulating distribution and functions of bacterial community along vertical soil profiles in red soil of pomelo orchard
Authors: Muneer Muhammad Atif , Hou Wei , Li Jian , Huang Xiaoman , ur Rehman Kayani Masood , Cai Yuanyang , Yang Wenhao , Wu Liangquan , Ji Baoming , Zheng Chaoyuan
Published: February 2022

Background

Soil microbes exist throughout the soil profile and those inhabiting topsoil (0–20 cm) are believed to play a key role in nutrients cycling. However, the majority of the soil microbiology studies have exclusively focused on the distribution of soil microbial communities in the topsoil, and it remains poorly understood through the subsurface soil profile (i.e., 20–40 and 40–60 cm). Here, we examined how the bacterial community composition and functional diversity changes under intensive fertilization across vertical soil profiles [(0–20 cm (RS1), 20–40 cm (RS2), and 40–60 cm (RS3)] in the red soil of pomelo orchard, Pinghe County, Fujian, China.

Results

Bacterial community composition was determined by 16S rRNA gene sequencing and interlinked with edaphic factors, including soil pH, available phosphorous (AP), available nitrogen (AN), and available potassium (AK) to investigate the key edaphic factors that shape the soil bacterial community along with different soil profiles. The most dominant bacterial taxa were Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, Crenarchaeota, and Bacteriodetes. Bacterial richness and diversity was highest in RS1 and declined with increasing soil depth. The distinct distribution patterns of the bacterial community were found across the different soil profiles. Besides, soil pH exhibited a strong influence (pH ˃AP ˃AN) on the bacterial communities under all soil depths. The relative abundance of Proteobacteria, Actinobacteria, Crenarchaeota, and Firmicutes was negatively correlated with soil pH, while Acidobacteria, Chloroflexi, Bacteriodetes, Planctomycetes, and Gemmatimonadetes were positively correlated with soil pH. Co-occurrence network analysis revealed that network topological features were weakened with increasing soil depth, indicating a more stable bacterial community in the RS1. Bacterial functions were estimated using FAPROTAX and the relative abundance of functional bacterial community related to metabolic processes, including C-cycle, N-cycle, and energy production was significantly higher in RS1 compared to RS2 and RS3, and soil pH had a significant effect on these functional microbes.

Conclusions

This study provided the valuable findings regarding the structure and functions of bacterial communities in red soil of pomelo orchards, and highlighted the importance of soil depth and pH in shaping the soil bacterial population, their spatial distribution and ecological functioning. These results suggest the alleviation of soil acidification by adopting integrated management practices to preserve the soil microbial communities for better ecological functioning.
Sepsis due to kidney injury caused by a toothpick: a case report and literature review
Authors: Qi Qi , Chen Lingxin , Kou Guoxian
Published: February 2022

Background

Toothpicks are common foreign bodies which may injure surrounding organs leading to a series of atypical symptoms. We present a rare clinical case that septicemia caused by a toothpick penetrated into the right kidney.

Case presentation

We describe a 51-year-old patient who presented with right-sided backache and hematuresis for 2 days. Blood culture persistently grew Streptococcus gordonii. Ultrasound of the patient’s urinary tract revealed a strong striated echo in the middle of the right kidney. Complete abdominal computed tomography revealed a duodenal foreign body penetrating into the right kidney. The toothpick was removed under endoscopy and hemostasis was given. Antibiotic treatment was upgraded. The patient was recovered and discharged from his stay on the fifteenth day.

Conclusions

Early identification of the etiology of sepsis can effectively alleviate patient’s distress and reduce hospital stay. Clinicians should identify the source of sepsis through a medical history and examination.
Albumin levels predict mortality in sepsis patients with acute kidney injury undergoing continuous renal replacement therapy: a secondary analysis based on a retrospective cohort study
Authors: Sheng Song , Zhang Yan-Hong , Ma Hang-Kun , Huang Ye
Published: February 2022

Background

Albumin (ALB) levels are negatively associated with mortality in patients with sepsis. However, among sepsis patients with acute kidney injury (AKI) undergoing continuous renal replacement therapy (CRRT), there has been no similar study on the correlation between ALB levels and mortality alone. This study tested the hypothesis that ALB levels are negatively associated with mortality among such patients.

Methods

We conducted a secondary analysis of 794 septic patients who were diagnosed with AKI and underwent CRRT in South Korea. For the Kaplan–Meier survival analysis, Cox proportional hazards models were used to study the hypotheses, with adjustments for the pertinent covariables. We also explore the possible nonlinear relationship and conducted sensitivity analyses including subgroup analyses and tests for interactions to investigate the association further. Additionally, ALB was used to construct model and we then compared the performance of ALB with that of APACHE II and SOFA in predicting mortality.

Results

The ALB level was an independent prognostic factor for death at 28 and 90 days after CRRT initiation (HR = 0.75, 95% CI: 0.62–0.90,P = 0.0024 for death at 28 days andHR = 0.73, 95% CI: 0.63–0.86,P < 0.0001 for death at 90 days). A nonlinear association was not identified between ALB levels and the endpoints. Subgroup analyses and tests for interactions indicated that HCO3and CRP played an interactive role in the association. ROC analysis indicated ALB, SOFA and APACHE-II were separately inadequate for clinical applications.

Conclusion

A 1 g/dL increase in ALB levels was independently associated with a 25 and 27% decrease in the risk of death at 28 and 90 days, respectively. However, this conclusion needs to be taken with caution as this study has several limitations.
“This is hard to cope with”: the lived experience and coping strategies adopted amongst Australian women with pelvic girdle pain in pregnancy
Authors: Ceprnja Dragana , Chipchase Lucinda , Liamputtong Pranee , Gupta Amitabh
Published: February 2022

Background

Women with pregnancy-related pelvic girdle pain (PPGP) report diminished ability to perform physical activities and experience higher rates of mood disorders, such as anxiety and depression, than pregnant women without PPGP. Despite these physical and psychological impacts, little is known about the lived experiences of PPGP amongst Australian women and the ways in which they cope. Situated within biographical disruption and social support theories, this study sought to gain a conceptual understanding of the experience and impact of PPGP on daily life, and how women cope with this condition during pregnancy.

Methods

A qualitative research design, situated within a phenomenological framework, using individual, semi-structured interviews consisting of open-ended questions was used with a flexible and responsive approach. Purposive sampling of pregnant women attending a single hospital included 20 participants between 14 and 38 weeks gestation, classified with PPGP as per recommended guidelines, with a mean (SD) age of 31.37 (4.16) years. Thematic analysis was performed where interview data was transcribed, coded, grouped into meaningful categories and then constructed into broad themes.

Results

Three themes were identified: 1. a transformed biography; 2. coping strategies; and 3. what women want. The pain experienced created a dramatic change in women’s lives, making the pregnancy difficult to endure. Women utilised social support, such as family, to help them cope with pain, and a self-care approach to maintain a positive mindset and reduce stress. Although a few women received support from healthcare professionals, many reported a lack information on PPGP and limited societal recognition of the condition. Women wanted early education, personalised information and prompt referral to help them cope with PPGP.

Conclusions

Findings from this study highlighted the complexity of living with PPGP as women attempted to deal with the unexpected impact on daily life by seeking support from partners and families, while also struggling with societal expectations. Although women with PPGP used a number of coping strategies, they sought greater support from healthcare professionals to effectively manage PPGP. These findings have important implications for the provision of health care to women living with PPGP.

Trial registration

Australian New Zealand Clinical Trials Registry: ACTRN12618001423202.
Novel multifunctional robotically assisted bipolar instrument for simultaneous radiofrequency sealing and transection: preclinical and single-center experience
Authors: Ibanez Jimenez Cristina , Lath Adrit , Ringold Forrest
Published: February 2022

Background

A novel robotic-assisted bipolar radiofrequency (RF) multifunctional vessel seal-and-transection instrument (SynchroSeal, Intuitive Surgical, Inc., Sunnyvale, CA) has been developed. The objective of the current paper is to describe the design of SynchroSeal based on bench studies, assess the safety of SynchroSeal in ex vivo and in vivo porcine studies, and provide early clinical context.

Methods

SynchroSeal grasping, energy activation time, and jaw temperature were evaluated with those of the Harmonic Ace+7. Data were analyzed with descriptive statistics, with Mann–Whitney for comparisons and statistical significance p < 0.05. Ex vivo and in vivo animal safety assessments of tissue after SynchroSeal use were evaluated for burst pressure, thermal spread, and acute sealing. Last, a single-center analysis of the technical metrics of SynchroSeal and Vessel Sealer Extend (robotically assisted seal-and-transection instrument) in bariatric cases is provided.

Results

Bench studies of SynchroSeal and Harmonic Ace+7 evidenced SynchroSeal’s greater slip resistance force (8.4 ± 1.0 vs. 3.1 ± 0.4 N; p = 0.0002), lower grip pressure (3.0 ± 0.2 vs. 4.2 ± 0.5 kg/cm2; p = 0.0002), faster seal time (1.5 ± 0.4 vs. 11.6 ± 2.5 s; p < 0.0001), lower mean jaw temperature (109.7 ± 7.2 vs. 247.4 ± 8.6 °C; p = 0.0051), and faster cooling to 40 °C (53.6 ± 2.1 vs. 68.0 ± 3.5 s; p = 0.0051). SynchroSeal’s mean burst pressures after seal-and-transection and seal only modes were, respectively, 1169.1 ± 393.1 mmHg and 1159.2 ± 454.6 mmHg. Mean thermal spreads were, respectively, 1.2 ± 0.6 mm and 1.5 ± 0.55 mm. In the chronic animal study, 102 vessels were sealed; at 3 weeks post-procedure, there was no evidence of leakage or adverse events, such as non-target tissue thermal spread or tissue damage. In bariatrics cases, SynchroSeal was activated more frequently per case; however, its mean activation time was significantly shorter than Vessel Sealer Extend. No adverse events were reported for either device.

Conclusions

SynchroSeal’s multifunctional design provides enhanced sealing and transection capabilities with an acceptable safety profile.
Predicting the diagnosis of prostate cancer with a scoring system based on novel biomarkers
Authors: Jethwani Durvesh Lachman , Sivamoorthy Lameena Lalitha , Toh Charng Chee , Malek Rohan
Published: February 2022

Objective

To predict prostate cancer using novel biomarker ratios and create a predictive scoring system.

Materials and methods

Data of a total of 703 patients who consulted Urology Department of Selayang Hospital between January 2013 and December 2017 and underwent prostate biopsy were screened retrospectively. Prostate specific antigen (PSA) levels, prostate volumes (PV), neutrophil and lymphocyte counts, neutrophil-to-lymphocyte ratio (NLR), Prostate specific antigen density (PSAD) and histopathology were evaluated.

Results

Ages ranged from 43 to 89 years, divided into 2 groups as per biopsy results; positive for prostate cancer (n = 290, 41.3%) and negative for malignancy (n = 413; 58.7%). Intergroup comparative evaluations were performed. Independent variables with p < 0.001 in the univariate analysis were age, DRE, PV, NLR, PSAD. A scoring system was modelled using NLR < 0.9, PSAD > 0.4, Age > 70 and DRE. A score of 2 or more predicted prostate cancer with a Sensitivity of 83.8% and Specificity of 86.4%.

Conclusions

NLR is shown to be good predictor for prostate cancer its usage in this scoring system affords more disease specificity as compared to PSA alone.
Value of normative belief in intention to use workplace health promotion apps
Authors: Junker Maren , Böhm Markus , Franz Mareike , Fritsch Tobias , Krcmar Helmut
Published: February 2022

Background

Mobile applications (apps) have started to be used for workplace health promotion (WHP). However, the factors that lead to the usage of apps in the workplace from the end-user perspective remain unclear.

Methods

To investigate the research gap, the study evaluates a model for the adoption of WHP apps by combining the theory of planned behavior, the health belief model, and the technology acceptance model. A self-administered questionnaire with validated scales among 354 participants was used to evaluate the proposed model for WHP.

Results

Although the study indicated a limited overall model fit, interesting aspects were derived. In particular, the study demonstrated that normative belief (especially), perceived usefulness, and attitudinal belief play important roles in the intention to use WHP apps.

Conclusion

The study is among the first to validate the theoretical models of mHealth adoption for WHP. Moreover, it shows that not only normative belief but also adjustment to several target groups is a necessary factor to be considered in the development and implementation of an app for WHP.
The contribution of benchmarking to quality improvement in healthcare. A systematic literature review
Authors: Willmington Claire , Belardi Paolo , Murante Anna Maria , Vainieri Milena
Published: February 2022

Background

Benchmarking has been recognised as a valuable method to help identify strengths and weaknesses at all levels of the healthcare system. Despite a growing interest in the practice and study of benchmarking, its contribution to quality of care have not been well elucidated. As such, we conducted a systematic literature review with the aim of synthesizing the evidence regarding the relationship between benchmarking and quality improvement. We also sought to provide evidence on the associated strategies that can be used to further stimulate quality improvement.

Methods

We searched three databases (PubMed, Web of Science and Scopus) for articles studying the impact of benchmarking on quality of care (processes and outcomes). Following assessment of the articles for inclusion, we conducted data analysis, quality assessment and critical synthesis according to the PRISMA guidelines for systematic literature review.

Results

A total of 17 articles were identified. All studies reported a positive association between the use of benchmarking and quality improvement in terms of processes (N = 10), outcomes (N = 13) or both (N = 7). In the majority of studies (N = 12), at least one intervention, complementary to benchmarking, was undertaken to stimulate quality improvement. The interventions ranged from meetings between participants to quality improvement plans and financial incentives. A combination of multiple interventions was present in over half of the studies (N = 10).

Conclusions

The results generated from this review suggest that the practice of benchmarking in healthcare is a growing field, and more research is needed to better understand its effects on quality improvement. Furthermore, our findings indicate that benchmarking may stimulate quality improvement, and that interventions, complementary to benchmarking, seem to reinforce this improvement. Although this study points towards the benefit of combining performance measurement with interventions in terms of quality, future research should further analyse the impact of these interventions individually.
Angiogenic content of microparticles in patients with diabetes and coronary artery disease predicts networks of endothelial dysfunction
Authors: Marei Isra , Chidiac Omar , Thomas Binitha , Pasquier Jennifer , Dargham Soha , Robay Amal , Vakayil Muneera , Jameesh Mohammad , Triggle Christopher , Rafii Arash , Jayyousi Amin , Al Suwaidi Jassim , Abi Khalil Charbel
Published: February 2022

Background

Elevated endothelial microparticles (EMPs) levels are surrogate markers of vascular dysfunction. We analyzed EMPs with apoptotic characteristics and assessed the angiogenic contents of microparticles in the blood of patients with type 2 diabetes (T2D) according to the presence of coronary artery disease (CAD).

Methods

A total of 80 participants were recruited and equally classified as (1) healthy without T2D, (2) T2D without cardiovascular complications, (3) T2D and chronic coronary artery disease (CAD), and (4) T2D and acute coronary syndrome (ACS). MPs were isolated from the peripheral circulation, and EMPs were characterized using flow cytometry of CD42 and CD31. CD62E was used to determine EMPs’ apoptotic/activation state. MPs content was extracted and profiled using an angiogenesis array.

Results

Levels of CD42- CD31 + EMPs were significantly increased in T2D with ACS (257.5 ± 35.58) when compared to healthy subjects (105.7 ± 12.96, p < 0.01). There was no significant difference when comparing T2D with and without chronic CAD. The ratio of CD42-CD62 +/CD42-CD31 + EMPs was reduced in all T2D patients, with further reduction in ACS when compared to chronic CAD, reflecting a release by apoptotic endothelial cells. The angiogenic content of the full population of MPs was analyzed. It revealed a significant differential expression of 5 factors in patients with ACS and diabetes, including TGF-β1, PD-ECGF, platelet factor 4, serpin E1, and thrombospondin 1. Ingenuity Pathway Analysis revealed that those five differentially expressed molecules, mainly TGF-β1, inhibit key pathways involved in normal endothelial function. Further comparison of the three diabetes groups to healthy controls and diabetes without cardiovascular disease to diabetes with CAD identified networks that inhibit normal endothelial cell function. Interestingly, DDP-IV was the only differentially expressed protein between chronic CAD and ACS in patients with diabetes.

Conclusion

Our data showed that the release of apoptosis-induced EMPs is increased in diabetes, irrespective of CAD, ACS patients having the highest levels. The protein contents of MPs interact in networks that indicate vascular dysfunction.
RNA-seq of nine canine prostate cancer cell lines reveals diverse therapeutic target signatures
Authors: Packeiser Eva-Maria , Taher Leila , Kong Weibo , Ernst Mathias , Beck Julia , Hewicker-Trautwein Marion , Brenig Bertram , Schütz Ekkehard , Murua Escobar Hugo , Nolte Ingo
Published: February 2022

Background

Canine prostate adenocarcinoma (PAC) and transitional cell carcinoma (TCC) are typically characterized by metastasis and chemoresistance. Cell lines are important model systems for developing new therapeutic strategies. However, as they adapt to culturing conditions and undergo clonal selection, they can diverge from the tissue from which they were originally derived. Therefore, a comprehensive characterization of cell lines and their original tissues is paramount.

Methods

This study compared the transcriptomes of nine canine cell lines derived from PAC, PAC metastasis and TCC to their respective original primary tumor or metastasis tissues. Special interests were laid on cell culture-related differences, epithelial to mesenchymal transition (EMT), the prostate and bladder cancer pathways, therapeutic targets in the PI3K-AKT signaling pathway and genes correlated with chemoresistance towards doxorubicin and carboplatin.

Results

Independent analyses for PAC, PAC metastasis and TCC revealed 1743, 3941 and 463 genes, respectively, differentially expressed in the cell lines relative to their original tissues (DEGs). While genes associated with tumor microenvironment were mostly downregulated in the cell lines, patient-specific EMT features were conserved. Furthermore, examination of the prostate and bladder cancer pathways revealed extensive concordance between cell lines and tissues. Interestingly, all cell lines preserved downstream PI3K-AKT signaling, but each featured a unique therapeutic target signature. Additionally, resistance towards doxorubicin was associated with G2/M cell cycle transition and cell membrane biosynthesis, while carboplatin resistance correlated with histone, m- and tRNA processing.

Conclusion

Comparative whole-transcriptome profiling of cell lines and their original tissues identifies models with conserved therapeutic target expression. Moreover, it is useful for selecting suitable negative controls, i.e., cell lines lacking therapeutic target expression, increasing the transfer efficiency from in vitro to primary neoplasias for new therapeutic protocols. In summary, the dataset presented here constitutes a rich resource for canine prostate and bladder cancer research.