bluuebunny/crossref_metadata_2025 huggingface dataset:
The Activation of Muscarinic Acetylcholine Receptors Protects against Neuroinflammation in a Mouse Model through Attenuating Microglial Inflammation
Authors: Wang Kaichun , Xie Yuanyuan , Chen Xixiang , Ouyang Xiaoyan , Zhao Lanxue , Chen Hongzhuan , Xu Jianrong
Published: September 2024

Neuroinflammation is a critical factor that contributes to neurological impairment and is closely associated with the onset and progression of neurodegenerative diseases. In the central nervous system (CNS), microglia play a pivotal role in the regulation of inflammation through various signaling pathways. Therefore, mitigating microglial inflammation is considered a promising strategy for restraining neuroinflammation. Muscarinic acetylcholine receptors (mAChRs) are widely expressed in the CNS and exhibit clear neuroprotective effects in various disease models. However, whether the activation of mAChRs can harness benefits in neuroinflammation remains largely unexplored. In this study, the anti-inflammatory effects of mAChRs were found in a neuroinflammation mouse model. The expression of various cytokines and chemokines was regulated in the brains and spinal cords after the administration of mAChR agonists. Microglia were the primary target cells through which mAChRs exerted their anti-inflammatory effects. The results showed that the activation of mAChRs decreased the pro-inflammatory phenotypes of microglia, including the expression of inflammatory cytokines, morphological characteristics, and distribution density. Such anti-inflammatory modulation further exerted neuroprotection, which was found to be even more significant by the direct activation of neuronal mAChRs. This study elucidates the dual mechanisms through which mAChRs exert neuroprotective effects in central inflammatory responses, providing evidence for their application in inflammation-related neurological disorders.
Testing Green Tea Extract and Ammonium Salts as Stimulants of Physical Performance in a Forced Swimming Rat Experimental Model
Authors: Korf Ekaterina A. , Novozhilov Artem V. , Mindukshev Igor V. , Glotov Andrey S. , Kudryavtsev Igor V. , Baidyuk Ekaterina V. , Dobrylko Irina A. , Voitenko Natalia G. , Voronina Polina A. , Habeeb Samarmar , Ghanem Afrah , Osinovskaya Natalia S. , Serebryakova Maria K. , Krivorotov Denis V. , Jenkins Richard O. , Goncharov Nikolay V.
Published: September 2024

The study of drugs of natural origin that increase endurance and/or accelerate recovery is an integral part of sports medicine and physiology. In this paper, decaffeinated green tea extract (GTE) and two ammonium salts—chloride (ACL) and carbonate (ACR)—were tested individually and in combination with GTE as stimulants of physical performance in a forced swimming rat experimental model. The determined parameters can be divided into seven blocks: functional (swimming duration); biochemistry of blood plasma; biochemistry of erythrocytes; hematology; immunology; gene expression of slow- and fast-twitch muscles (m. soleus, SOL, and m. extensor digitorum longus, EDL, respectively); and morphometric indicators of slow- and fast-twitch muscles. Regarding the negative control (intact animals), the maximum number of changes in all blocks of indicators was recorded in the GTE + ACR group, whose animals showed the maximum functional result and minimum lactate values on the last day of the experiment. Next, in terms of the number of changes, were the groups ACR, ACL, GTE + ACL, GTE and NaCl (positive control). In general, the number of identified adaptive changes was proportional to the functional state of the animals of the corresponding groups, in terms of the duration of the swimming load in the last four days of the experiment. However, not only the total number but also the qualitative composition of the identified changes is of interest. The results of a comparative analysis suggest that, in the model of forced swimming we developed, GTE promotes restoration of the body and moderate mobilization of the immune system, while small doses of ammonium salts, especially ammonium carbonate, contribute to an increase in physical performance, which is associated with satisfactory restoration of skeletal muscles and the entire body. The combined use of GTE with ammonium salts does not give a clearly positive effect.
In Silico Structural Prediction for the Generation of Novel Performant Midi-Dystrophins Based on Intein-Mediated Dual AAV Approach
Authors: Palmieri Laura , Ferrand Maxime , Vu Hong Ai , Richard Isabelle , Albini Sonia
Published: September 2024

Duchenne Muscular Dystrophy (DMD) is a pediatric disorder characterized by progressive muscle degeneration and premature death, and has no current cure. The current, most promising therapeutic avenue is based on gene replacement mediated by adeno-associated viruses (AAVs) using a shortened, but still functional, version of dystrophin, known as micro-dystrophin (µDys), to fit AAV capacity. The limited improvements observed in clinical trials suggest a sub-optimal performance of µDys in the human context that could be due to the lack of key domains in the protein. Therefore, expressing larger dystrophin proteins may be necessary for a more complete correction of the disease phenotype. In this study, we developed three novel midi-dystrophin constructs using a dual-AAV approach, leveraging split-intein-based protein trans-splicing. The midi-dystrophins include additional domains compared to µDys, such as the central cytoskeleton-binding domain, nNOS and Par1b interacting domains, and a complete C-terminal region. Given the limited capacity of each AAV vector, we strategically partially reduced hinge regions while ensuring that the structural stability of the protein remains intact. We predicted the interactions between the two halves of the split midi-Dys proteins thanks to the deep learning algorithm AphaFold3. We observed strong associations between the N- and C-termini in midi-Dys 1 and 2, while a weaker interaction in midi-Dys 3 was revealed. Our subsequent experiments confirmed the efficient protein trans-splicing both in vitro and in vivo in DBA2/mdx mice of the midi-Dys 1 and 2 and not in midi-Dys 3 as expected from the structural prediction. Additionally, we demonstrated that midi-Dys 1 and 2 exhibit significant therapeutic efficacy in DBA2/mdx mice, highlighting their potential as therapeutic agents for DMD. Overall, these findings highlight the potential of deep learning-based structural modeling for the generation of intein-based dystrophin versions and pose the basis for further investigation of these new midi-dystrophins versions for clinical studies.
Developing 1,4-Diethyl-1,2,3,4-tetrahydroquinoxalin-substituted Fluorogens Based on GFP Chromophore for Endoplasmic Reticulum and Lysosome Staining
Authors: Rudik Daniil I. , Perfilov Maxim M. , Sokolov Anatolii I. , Chen Cheng , Baleeva Nadezhda S. , Myasnyanko Ivan N. , Mishin Alexander S. , Fang Chong , Bogdanova Yulia A. , Baranov Mikhail S.
Published: September 2024

In the present study, we demonstrated that the introduction of a 1,4-diethyl-1,2,3,4-tetrahydroquinoxalin moiety into the arylidene part of GFP chromophore-derived compounds results in the formation of environment-sensitive fluorogens. The rationally designed and synthesized compounds exhibit remarkable solvent- and pH-dependence in fluorescence intensity. The solvent-dependent variation in fluorescence quantum yield makes it possible to use some of the proposed compounds as polarity sensors suitable for selective endoplasmic reticulum fluorescent labeling in living cells. Moreover, the pH-dependent emission intensity variation of other fluorogens makes them selective fluorescent labels for the lysosomes in living cells.
Organoid Models in Cancer Immunotherapy: Bioengineering Approach for Personalized Treatment
Authors: Noorintan Syarifah Tiara , Angelius Chelsie , Torizal Fuad Gandhi
Published: September 2024

Recent advances in organoid technology have revolutionized cancer biology and therapeutic interventions, offering personalized immunotherapy treatment. Organoids, three-dimensional cell cultures derived from patient tumors, accurately replicate the tumor microenvironment, providing unprecedented insights into tumor-immune interactions and therapeutic responses. In this literature-based study, we discuss various culture methods for the diverse applications of organoids in cancer immunotherapy, including drug screening, personalized treatment strategies, and mechanistic studies. Additionally, we address the technological challenges associated with these methods and propose potential future solutions to accelerate the development of novel immunotherapeutic approaches. This review highlights the transformative potential of organoid models in advancing preclinical cancer immunotherapy modeling, screening, and evaluation, paving the way for more effective and personalized cancer treatments.
AI-Enhanced Decision-Making for Course Modality Preferences in Higher Engineering Education during the Post-COVID-19 Era
Authors: Mehrabi Amirreza , Morphew Jason Wade , Araabi Babak Nadjar , Memarian Negar , Memarian Hossein
Published: September 2024

The onset of the COVID-19 pandemic has compelled a swift transformation in higher-education methodologies, particularly in the domain of course modality. This study highlights the potential for artificial intelligence and machine learning to improve decision-making in advanced engineering education. We focus on the potential for large existing datasets to align institutional decisions with student and faculty preferences in the face of rapid changes in instructional approaches prompted by the COVID-19 pandemic. To ascertain the preferences of students and instructors regarding class modalities across various courses, we utilized the Cognitive Process-Embedded Systems and e-learning conceptual framework. This framework effectively delineates the task execution process within the scope of technology-enhanced learning environments for both students and instructors. This study was conducted in seven Iranian universities and their STEM departments, examining their preferences for different learning styles. After analyzing the variables by different feature selection methods, we used three ML methods—decision trees, support vector machines, and random forest—for comparative analysis. The results demonstrated the high performance of the RF model in predicting curriculum style preferences, making it a powerful decision-making tool in the evolving post-COVID-19 educational landscape. This study not only demonstrates the effectiveness of ML in predicting educational preferences but also contributes to understanding the role of self-regulated learning in educational policy and decision-making in higher education.
Orofacial Migraine—A Narrative Review
Authors: Benoliel Rafael , May Arne
Published: September 2024

The diagnosis of migraine is based on clear criteria outlined in the International Classification of Headache Disorders version 3 (ICHD-3). Notably, the criteria in ICHD-3 omit the location of the migraine. There are increasing reports of migraine in the facial region. Facial presentations of migraine are not easy to diagnose as they appear in the lower two-thirds of the face, often in the maxillary sinus region, around the ear, the upper/lower jaws, and the teeth. Additionally, a similar but distinct entity, neurovascular orofacial pain, has been established. The symptomatology of facial presentations of these headaches often resembles sinusitis and dental pathology. We will review these presentations, their diagnosis, and possible pathophysiology.
Pre- and Procedural Factors Influencing the Success of In Vitro Fertilization: Evaluating Embryo Quality and Clinical Pregnancy in Cases of Tubal Factor Infertility
Authors: Kozinszky Zoltan , Bereczki Kristóf , Vedelek Viktor , Bicskei Petra , Tabi Mariann , Ekes Csaba , Lajkó Noémi , Nagy Olga , Sinka Rita , Vágvölgyi Anna , Zádori János
Published: September 2024

Introduction: While tubal occlusion is a prevalent cause of infertility, accounting for 11–35% of infertility cases among women, there remains a limited understanding of the factors influencing clinical pregnancy following in vitro fertilization (IVF). Methods: In our retrospective, cross-sectional cohort study conducted at a single tertiary center, medical records of women aged 19 to 43 years were analyzed. Logistic regression models were employed to identify the prognostic factors associated with clinical pregnancy after IVF in patients with tubal factor infertility, excluding cases with hydrosalpinx. Results: Data from 219 women diagnosed with tubal occlusion were compared to 1140 cases with non-tubal indication, covering a total of 1359 IVF cycles. A lower maternal age (adjusted odds ratio [AOR]: 0.89, p = 0.001) and a higher embryo quality (AOR: 1.26, p = 0.01) emerged as important factors in clinical pregnancy in the tubal infertility group. Moreover, a lower maternal (AOR:0.91, p < 0.01) and paternal age (p = 0.001), and favorable semen quality (AOR: 1.32, p = 0.03) were critical determinants in the non-tubal infertility group. BMI was generally higher in tubal infertility patients (p = 0.01). Furthermore, FSH level (AOR: 0.93, p = 0.004), AMH level (p < 0.04), number of embryos transferred (AOR: 2.04, p < 0.001), and embryo quality (AOR: 1.26, p < 0.001) came into prominence only in the non-tubal infertility group. The clinical pregnancy rate (34.2%) of women with tubal occlusion did not differ significantly from those in other forms of infertility undergoing IVF (35.4%). Conclusions: Although tubal infertility is typically anticipated to yield the highest clinical pregnancy rates following IVF, it is crucial to acknowledge that both maternal and paternal characteristics can also significantly impact the outcomes.
Submaximal Verification Test to Exhaustion Confirms Maximal Oxygen Uptake: Roles of Anaerobic Performance and Respiratory Muscle Strength
Authors: Michalik Kamil , Danek Natalia
Published: September 2024

Background: The incremental exercise test is commonly used to measure maximal oxygen uptake (VO2max), but an additional verification test is often recommended as the “gold standard” to confirm the true VO2max. Therefore, the aim of this study was to compare the peak oxygen uptake (VO2peak) obtained in the ramp incremental exercise test and that in the verification test performed on different days at submaximal intensity. Additionally, we examined the roles of anaerobic performance and respiratory muscle strength. Methods: Sixteen physically active men participated in the study, with an average age of 22.7 ± 2.4 (years), height of 178.0 ± 7.4 (cm), and weight of 77.4 ± 7.3 (kg). They performed the three following tests on a cycle ergometer: the Wingate Anaerobic Test (WAnT), the ramp incremental exercise test (IETRAMP), and the verification test performed at an intensity of 85% (VER85) maximal power, which was obtained during the IETRAMP. Results: No significant difference was observed in the peak oxygen uptake between the IETRAMP and VER85 (p = 0.51). The coefficient of variation was 3.1% and the Bland–Altman analysis showed a high agreement. We found significant correlations between the total work performed in the IETRAMP, the anaerobic peak power (r = 0.52, p ≤ 0.05), and the total work obtained in the WAnT (r = 0.67, p ≤ 0.01). There were no significant differences in post-exercise changes in the strength of the inspiratory and expiratory muscles after the IETRAMP and the VER85. Conclusions: The submaximal intensity verification test performed on different days provided reliable values that confirmed the real VO2max, which was not limited by respiratory muscle fatigue. This verification test may be suggested for participants with a lower anaerobic mechanical performance.
Neoadjuvant Treatment in Locally Advanced Thyroid Carcinoma
Authors: Navas Moreno Víctor , Sebastián Valles Fernando , Lahera Vargas Marcos , Hernández Marín Berta , Carrillo López Elena , Marazuela Mónica , Muñoz de Nova José Luis
Published: September 2024

Although the focus in the last decades has been on the overdiagnosis of incidentally detected thyroid carcinomas in early stages, the other extreme of the disease is represented by locally advanced tumors with the invasion of neighboring structures. These are infrequent tumors, but they have a high complexity and a poor prognosis. In the absence of effective therapies allowing preoperative tumor reduction, in order to achieve a more restricted surgery, treatment was limited to aggressive surgery with resection of the aerodigestive tract and major vascular structures or palliative treatment. However, due to the increased knowledge of tumor biology and the results that tyrosine kinase inhibitors have achieved in the treatment of radioactive iodine-refractory tumors, neoadjuvant therapy with a curative intent has emerged as a reality to be taken into account when dealing with these patients. This paper presents a narrative review of the current scientific evidence regarding neoadjuvant treatment in locally advanced thyroid cancer.
Crosslinked All-Femtosecond Laser-Cut Corneal Allogenic Intracorneal Ring Segments (AFXL CAIRSs): Pilot Ex Vivo Study and Report of First Two Cases Performed in Italy
Authors: Mazzotta Cosimo , Zagari Marco , Bona Giulia , Ponzin Diego , Awwad Shady T. , Torres-Netto Emilio A. , Hafezi Farhad , Jacob Soosan
Published: September 2024

Objectives: This pilot ex vivo study and first clinical experience in Italy evaluate the impact of using pre-implantation crosslinking on all-femtosecond laser-cut corneal allogenic intracorneal ring segments (AFXL CAIRSs). Methods: Six human donor eye-bank corneas were used for this preclinical ex vivo human study. Three donor (D) corneas were used for AFXL CAIRSs. First, they were prepared with an IntraLase™ femtosecond laser (Johnson & Johnson, New Brunswick, NJ, USA). The allogenic tissue rings were crosslinked before implantation with Riboflavin–UV-A accelerated crosslinking protocol (ACXL) with a 0.1% HPMC Riboflavin isotonic solution (Vibex Rapid, Glaukos-Avedro, Burlington, MA, USA) and a new KXL UV-A emitter (Glaukos-Avedro, USA). Three corneas were used as recipients (Rs) of the AFXL CAIRSs. After completing the ex vivo phase, IRB approval and signing a specific informed consent, the first two Italian patients were treated. A single ACXL CAIRS was implanted in a 51-year-old male with 53.53 D K steep, 363 μm minimum corneal thickness (MCT) and a double ACXL CAIRS was implanted in a 46-year-old male patient with 58.30 D K steep, 443 μm MCT. The longest follow-up was at three months. Results: Crosslinking of the segments enhanced tissue stiffness and grip, facilitating manipulation and CAIRS insertion into the recipient tunnels, and the yellowish color of the crosslinked segments improved visibility. The segment’s thickness and volume remained unaltered during the follow-up. Both patients improved UDVA and BSCVA. K steep and High-Order Aberrations (HOAs) were reduced and MCT increased. Conclusions: Pre-implantation ACXL facilitated CAIRS insertion preserving dimensions and volume during the follow-up, rendering this important step a promising candidate in method standardization. Functional data and MCT improved significantly without adverse events.
Limitations of a Cross-Sectional Correlation Study. Comment on Elabd et al. Prediction of Back Disability Using Clinical, Functional, and Biomechanical Variables in Adults with Chronic Nonspecific Low Back Pain. J. Clin. Med. 2024, 13, 3980
Authors: Kovacs Francisco M. , Seco-Calvo Jesús
Published: September 2024

We hereby comment on a study recently published by J [...]
A Review on Asthma and Allergy: Current Understanding on Molecular Perspectives
Authors: Gohal Gassem , Moni Sivakumar S. , Bakkari Mohammed Ali , Elmobark Mohamed Eltaib
Published: September 2024

Asthma, a complex disease characterized by persistent airway inflammation, remains an urgent global health concern. We explored the critical role of allergic biomarkers and dysregulated immune system in asthma through an extensive literature review in databases such as Web of Science, PubMed, EMBASE, Scopus, and Google Scholar. This review summarizes the growing data on the pivotal role of allergic biomarkers and dysregulated immune system in the development and evolution of asthma. Recent studies have uncovered several biomarkers that elucidate intrinsic allergic mechanisms in individuals with asthma. This article highlights these biomarkers’ potential in predicting asthma onset, assessing its intensity, guiding therapeutic interventions, and tracking disease progression. We also explore the innovative therapeutic prospects arising from the convergence of allergy and dysregulated immune system in asthma and emphasize the potential for precision medicine approaches. Understanding allergic biomarkers intertwined with a dysregulated immune system heralds a new era in asthma treatment and points to improved and individualized treatment modalities.
Factors Affecting Intraoperative Blood Transfusion Requirements during Living Donor Liver Transplantation
Authors: Kilercik Hakan , Akbulut Sami , Elsarawy Ahmed , Aktas Sema , Alkara Utku , Sevmis Sinasi
Published: September 2024

Background: Intraoperative blood transfusion (IOBT) during liver transplantation (LT) has negative outcomes, and it has been shown that an increasing number of these procedures may no longer require IOBT. Regarding living donor liver transplantation (LDLT), the literature on the pre-transplant predictors of IOBT is quite heterogeneous and deficient. In this study, we reviewed our experience of IOBT among a homogenous cohort of adult right-lobe LDLTs. Methods: We conducted a retrospective analysis of prospectively collected data on adult LDLT recipients between January 2018 and October 2023. Two groups were constructed (No-IOBT vs. IOBT) for the exploration of pre- and intraoperative predictors of IOBT using univariate and multivariate analyses. An ROC curve analysis was applied to identify possible cut-offs. The one-year post-LDLT overall survival was compared using the Kaplan–Meier method. A p-value < 0.05 was considered statistically significant. Results: A total of 219 adult LDLT recipients were enrolled. The No-IOBT (n = 56) patients were mostly males (p = 0.016), with higher preoperative levels of HGB (p < 0.001), fibrinogen (p = 0.005), and albumin (p = 0.007) and a lower incidence of pre-transplant upper abdominal surgery (p = 0.017), portal vein thrombosis (p = 0.04), hepatorenal syndrome (p = 0.015), and ascites (p = 0.02) than the IOBT group (n = 163). The No-IOBT group had a shorter anhepatic phase (p = 0.002) and received fewer intravenous crystalloids (p = 0.001). In the multivariate analysis, the pre-transplant HGB (p < 0.001), fibrinogen (p < 0.001), and albumin (p = 0.04) levels were independent predictors of IOBT, showing the following cut-offs in the ROC curve analysis: HGB ≤ 11.5 (AUC: 0.800, p < 0.001), fibrinogen ≤ 125 (AUC: 0.638, p = 0.0024), and albumin ≤ 3.6 (AUC: 0.663, p = 0.0002). These were significantly associated with the No-IOBT group. The one-year overall survival of the No-IOBT and IOBT groups was 100% and 83%, respectively (p = 0.007). Conclusions: IOBT during LDLT is associated with inferior outcomes. The increased need of IOBT during LT can be predicted by evaluating serum levels of hemoglobin, albumin and fibrinogen before liver transplantation.
The Model for End-Stage Liver Disease (MELD) Score Predicting Mortality Due to SARS-CoV-2 in Mexican Patients
Authors: Reyes-Ruiz José Manuel , Avelino-Santiago Ana Citlali , Martínez-Mier Gustavo , López-López Claudia Vanessa , De Jesús-González Luis Adrián , León-Juárez Moises , Osuna-Ramos Juan Fidel , Farfan-Morales Carlos Noe , Palacios-Rápalo Selvin Noé , Bernal-Dolores Víctor , Del Ángel Rosa María
Published: September 2024

Background/Objectives: Coronavirus Disease 2019 (COVID-19) can cause liver injury and a deterioration of hepatic function. The Model for End-Stage Liver Disease (MELD) score is a good predictor for poor prognosis of hospitalized COVID-19 patients in the United States, Egypt and Turkey. Nevertheless, the best cut-off value for the MELD score to predict mortality in the Mexican population has yet to be established. Methods: A total of 234 patients with COVID-19 were studied in a tertiary-level hospital. Patients were stratified into survivors (n = 139) and non-survivors (n = 95). Receiver operating characteristic curves, Cox proportional hazard models, Kaplan–Meier method, and Bonferroni corrections were performed to identify the predictors of COVID-19 mortality. Results: MELD score had an area under the curve of 0.62 (95% CI: 0.56–0.68; p = 0.0009), sensitivity = 53.68%, and specificity = 73.38%. Univariate Cox proportional hazard regression analysis suggested that the leukocytes > 10.6, neutrophils > 8.42, neutrophil-to-lymphocyte ratio (NLR) > 8.69, systemic immune-inflammation index (SII) > 1809.21, MELD score > 9, and leukocyte glucose index (LGI) > 2.41 were predictors for mortality. However, the multivariate Cox proportional hazard model revealed that only the MELD score >9 (Hazard Ratio [HR] = 1.83; 95% confidence interval [CI]: 1.2–2.8; Pcorrected = 0.03) was an independent predictor for mortality of COVID-19. Conclusions: Although the MELD score is used for liver transplantation, we suggest that a MELD score >9 could be an accurate predictor for COVID-19 mortality at admission to ICU requiring mechanical ventilation.
Therapeutic Potential of Gum Arabic (Acacia senegal) in Chronic Kidney Disease Management: A Narrative Review
Authors: Alobaidi Sami
Published: September 2024

Chronic kidney disease (CKD) poses significant health challenges globally, particularly in regions like the Middle East. This review evaluates the potential efficacy and safety of Gum Arabic (Acacia senegal), a traditional remedy, in managing CKD. A comprehensive literature review was conducted using databases including PubMed and Scopus, focusing on the biochemical, physiological, and therapeutic impacts of Gum Arabic on renal health. Gum Arabic has demonstrated antioxidative and anti-inflammatory properties that may benefit renal function, as shown in animal studies. Clinical trials suggest improvements in renal biomarkers, though these are limited by scope and methodology. While promising, the clinical application of Gum Arabic requires cautious interpretation due to gaps in understanding its mechanisms of action. Gum Arabic shows potential as an adjunct treatment for CKD, reflecting both traditional use and preliminary scientific evidence. Future research should focus on its long-term efficacy, safety, and underlying biochemical pathways to better guide its therapeutic use.
Trends and Changes in Treating Proximal Humeral Fractures in Italy: Is Arthroplasty an Increasingly Preferred Option? A Nation-Wide, Population-Based Study over a Period of 22 Years
Authors: Ciminello Enrico , Modesti Andrea , Romanini Emilio , Lepore Stefano , Tucci Gabriele , Di Gennaro Stefano , Logroscino Giandomenico , Ciccarelli Paola , Falcone Tiziana , Torre Marina
Published: September 2024

Background: Proximal humeral fractures (PHFs) are common, especially in the elderly, and account for 4% to 10% of all fractures, with women more often affected than men. Treatments include conservative methods, internal fixation and arthroplasty, with surgical approaches increasingly being used due to technological advancements. This study analyzes the evolution of PHF treatments in Italy from 2001 to 2022, using data from the Italian Hospital Discharge Records (HDRs) Database, and includes a stratified analysis by age and sex. Methods: Using HDR data from 2001 to 2022, records with ICD9-CM codes for proximal humeral fractures (812.0 and 812.1) among diagnoses were selected and categorized into three treatment groups: arthroplasty, fixation and conservative. Time series were analyzed with stratification by sex and age. Results: The extracted data included 486,368 records of PHFs, with 223,742 cases treated surgically (arthroplasty or internal fixation) and 262,626 treated conservatively; the average patient age was 66.6 years, with a higher proportion of women, especially among arthroplasty patients. Over time, the use of fixation and arthroplasty increased from 20% of treatments in 2001 to over 60% in 2022, with fixation becoming the most common treatment method by 2014 and arthroplasty significantly increasing among women, particularly in the 65–74 and 75–84 age groups. Conclusions: The study shows that in Italy, over the past two decades, treatment for PHFs has shifted from conservative methods to a preference for internal fixation and increasingly for arthroplasty, particularly among women and patients aged 65–84, reflecting evolving trends and technological improvements.
Characteristics of Pinus hwangshanensis Rhizospheric Fungal Community along Huangshan Mountain’s Elevation Gradients, China
Authors: Zuo Qinglin , Dang Keke , Yin Jing , Yuan Dandan , Lu Jing , Xiang Xingjia
Published: September 2024

Elevation gradients strongly influence the diversity pattern of soil microorganisms. To date, many studies have elucidated the response of soil microbes to changes in elevation gradients. However, the effects of these gradients on the assembly mechanisms and network complexity of rhizospheric microbial communities remain underexplored. To bridge this knowledge gap, this study assessed the response of rhizospheric fungal communities of Pinus hwangshanensis along different elevation gradients in the Huangshan Mountain scenic area with regard to diversity, community composition, and assembly mechanisms using high-throughput amplicon sequencing. The results revealed significant differences in rhizospheric fungal community composition across three elevation gradients. The soil organic matter and pH were the most relevant factors influencing the changes in rhizospheric fungal community composition. The rhizospheric fungal diversity was significantly lower at both low and high elevations compared to the medium elevation. The rhizospheric fungal community assembly showed a more deterministic process at low and high elevations than at the medium elevation, indicating that stronger environmental filtering contributed to reduced fungal diversity at the extremes of the elevation gradient. In addition, rhizospheric pathogens, particularly Dermateaceae, acted as keystone taxa, diminishing the stability of co-occurrence networks at the medium elevation. This study contributes to a more comprehensive understanding of rhizospheric fungal community patterns and their ecological functions along elevation gradients in mountainous regions.
Disney Reloaded: Pixar’s Influence on the Evolution of Disney Animation Feature Films (1994–2018)
Authors: Luna Marta Izarra de , Marcos Roberto Gelado
Published: September 2024

Disney animation studios created and subsequently shaped the animation genre for the last two-thirds of the 20th century, but the appearance of Pixar in the industry and their unstoppable success changed the rules of the game. (1) Apart from a new and revolutionary technology, Pixar introduced a new type of storytelling in animation based on characters and stories that we believe ended up tremendously influencing Disney’s storytelling starting in 2006, when the big animation studio purchased its most threatening competitor. Our study also tries to shed some light on whether the changes happened only at the level of storytelling or also crystallized into better box office and rating data. (2) We aim to clarify this belief and turn it into a reality through the content analysis of Disney animation features before and after the purchase of Pixar. (3) Our results show that Pixar’s influence on Disney is remarkable, both in the movies’ narrative and in their reception by the audience and the critics. (4) This confirms not only the change in the story-telling strategies of the company, enhancing psychological construction of the protagonists of Disney animation features, but also the subsequent impact on its audiences.
Intercultural Attitudes Embedded in Microblogging: Sentiment and Content Analyses of Data from Sina Weibo
Authors: Zhang Xiaotian
Published: September 2024

This study analyzed 2421 microblogs posted between the year 2012 to March 2022 reflecting the microbloggers’ attitudes toward different cultures. Results indicated that (1) the number of microblog posts expressing the users’ intercultural attitudes increased distinctly from 2019 to March 2022, with females users in general posting more microblogs than males; (2) females posted more microblogs encompassing positive emotions to show their interest and motivation to learn about foreign cultures, and the tendency to value and appreciate cultural differences, whereas males created more sentimentally neutral posts that revealed their recognition of the existence of cultural differences, and females and males posted a similar number of microblogs containing negative emotions; and (3) more posts involved “small c” culture were posted than those containing themes belonging to the “Big C” culture. Gender gap was further observed regarding the cultural themes concerned by the microbloggers. Implications were discussed in the context of intercultural education.
Enhancing Differential Diagnosis Related to Oxidative Stress, Nitrous Oxide, and Nutrition by Rapid Plasma Homocysteine Measurement
Authors: Grzych Guillaume , Zerimech Farid , Touze Benjamin , Descamps Clarence , Bout Marie-Adélaïde , Joncquel Marie , Douillard Claire , Kim Isabelle , Tard Céline , Brousseau Thierry
Published: September 2024

Background: Historically used as a marker for inherited disorders, the current interest in plasma homocysteine measurement lies in its ability to provide valuable information about the metabolic and nutritional status of patients. Specifically, nitrous oxide (N2O) abuse can lead to functional vitamin B12 deficiency by oxidation and increase oxidative stress, resulting in elevated plasma homocysteine levels, which mimic neurological conditions such as Guillain–Barré syndrome. Rapid identification of hyperhomocysteinemia is crucial for timely intervention and avoiding costly, unnecessary treatments. Objective: This study evaluates the performance of a rapid immunoassay technique (Snibe) compared to mass spectrometry (LC-MS/MS) for measuring plasma homocysteine levels in patients with nitrous oxide abuse and non-inherited caused of elevated homocysteine, aiming to enhance differential diagnosis related to oxidative stress. Methods: 235 patients from Lille University Hospital were included. EDTA blood samples were collected and analyzed using both rapid immunoassay (Snibe) and LC-MS/MS. Neurological assessment was performed using the peripheral neuropathy disability (PND) score. Results: Firstly, significant elevations in plasma homocysteine levels were observed in patients abusing nitrous oxide measured by LC-MS/MS. Secondly, the immunoassay provided rapid results, essential for early clinical decision-making, but tended to underestimate high values compared to LC-MS/MS. A good correlation was found between the methods for low and moderate values. Conclusion: The immunoassay tended to underestimate high-value samples compared to LC-MS/MS, which is a common problem with the competitive methodology. The rapid immunoassay technique is effective for initial screening and early intervention, aiding in the differential diagnosis of conditions related to oxidative stress. Therefore, it is recommended to use the CLIA method for initial screening and confirm with mass spectrometry if there are abnormal samples. Integrating both techniques can enhance diagnostic accuracy and improve patient outcomes.
Strategic Interactions in Omnichannel Retailing: Analyzing Brand Competition and Optimal Strategy Selection
Authors: Yu Jing , Ren Yufei , Zhou Chi
Published: September 2024

The rapid advancement of digital technology has blurred the line between online and brick-and-mortar stores, leading to the proliferation of omnichannel retailing. Two widely adopted strategies are Buy Online and Pick Up in Store (BOPS) and Ship from Store (SFS). This study examines a supply chain where a manufacturer sells national brand products through an e-commerce platform (e-platform) that also offers its own brand products. We analyze the optimal omnichannel strategies for both the e-platform and the manufacturer, considering scenarios of cooperation and brand competition, across four strategy combinations. Our findings highlight that the profits of both the e-platform and manufacturer are primarily influenced by the commission rate, product category valuation and competition intensity. The commission rate plays a pivotal role in shaping the e-platform’s strategy: a low rate leads to direct competition with the manufacturer, while a high rate prioritizes the manufacturer’s products. When the spillover profit is less than the net difference between the customer’s additional benefits and the firm’s additional costs for SFS compared to BOPS, and the commission rate is high, an equilibrium is achieved. Sensitivity analyses reveal that as the product differentiation decrease, the manufacturer’s profits decline, while the e-platform’s profits rise with an increasing commission rate.
Wetlands in Crisis: The Silent Desertification Threat on the Greek Wetlands
Authors: Zotos Anastasios , Kokkoris Ioannis P. , Charalampopoulos Ioannis , Bekri Eleni S. , Dimopoulos Panayotis
Published: September 2024

This study deals with the information gap on desertification risk for wetland habitat types in Natura 2000 network sites of Greece. Using the Environmentally Sensitive Areas (ESA) index as a proxy, all Natura 2000 wetland habitat types have been assessed and assigned to desertification risk categories. The assessment was conducted at the national, regional, and local scales in order to provide different outcomes for targeted support on decision and policy making regarding restoration and conservation measures. The main results document that circa 20% of wetland habitat types area are considered under desertification risk, while circa 10% are considered as potentially affected by desertification. It was also shown that there should be prioritization of the habitat types that need attention due to their inclusion in the different desertification risk categories. The study also highlights the administrative regions (NUTS2) and Natura 2000 sites and the need to structure, draft, and implement conservation projects to mitigate wetlands’ risk as well as the use of wetlands as primary nature-based solutions (NbS) in the battle with desertification and climate change. Management implications are also provided from the perspective of habitat restoration as well as for their exploitation as valuable NbS for biodiversity conservation and ecosystem services maintenance.
The Interconnectedness of Land–Crops–Livestock and Environmental Quality in Emerging Asian Economies: Challenges of Agriculturalization and Carbonization
Authors: Rehman Abdul , Ulucak Recep , Ma Hengyun , Ding Jing , Hua Junguo
Published: September 2024

The release of greenhouse gases (GHGs) is a major contributor to global warming, endangering both human and nonhuman well-being, environmental integrity, economic development, and the planet’s long-term survival. This study delves into the interplay between crop production, livestock production, fertilizer utilization, and agricultural land usage on CO2 emissions in four Asian economies: China, India, Pakistan, and Bangladesh. Employing panel data analysis techniques, the research uncovers the significant impacts of various agricultural activities on environmental degradation. The findings derived from the panel autoregressive distributed lag (PARDL) estimation reveal that crop production in these emerging economies contributes to CO2 emissions, as evidenced by the positive coefficients and statistically significant results. Similarly, livestock production and agricultural land used for crop production exhibit a substantial impact on CO2 emissions, further highlighting their role in environmental degradation. While fertilizer usage also displays a positive coefficient, its impact on CO2 emissions is not statistically significant. The results of our study highlight the critical importance of addressing the environmental impacts of agricultural practices, particularly in emerging economies. Crop and livestock production, along with the expansion of agricultural land, significantly contribute to CO2 emissions, which underscores the urgent need for sustainable agricultural practices. These findings suggest that policymakers should prioritize the development and implementation of strategies that mitigate the environmental impacts of agriculture. This could include promoting sustainable land management practices, investing in technology that reduces emissions from crop and livestock production, and encouraging the adoption of eco-friendly fertilizers.
Communication Skills in Toddlers Exposed to Maternal SARS-CoV-2 during Pregnancy
Authors: Apa Enrico , Tegmeyer Nicole Carrie , D’Adamo Concetta , Lovati Eleonora , Cocchi Chiara , Allegra Paola , Ostello Francesco , Monzani Daniele , Genovese Elisabetta , Palma Silvia
Published: September 2024

Studies about the effects of SARS-CoV-2 on pregnant women and children born to positive women are controversial with regard to possible inner ear-related damage but most of them do not detect the involvement of this virus in auditory function. However, only a few studies on long-term effects on language development are currently available because of the recent onset of the pandemic. The aim of this study was to investigate the impact of SARS-CoV-2 infection on perceptual and expressive abilities and the emerging development of communication in young children. To this purpose, the MacArthur–Bates Communicative Development Inventory—Words and Gestures form (CDI-WG), was administered to parents. In total, 115 children whose mother was infected by SARS-CoV-2 during pregnancy were enrolled in the study and evaluated at the Audiology Service of the Modena University Hospital. All children underwent Otoacoustic Emissions (OAE) at birth: 114/115 had a “pass” result bilaterally, while 1 case had a unilateral “refer” result. Overall, 110/115 newborns (95.65%) underwent audiological evaluation between 10–18 months of age. In 5/110 patients (3.6%), the Pure Tone Average (PTA) result was equal to 35 dB; one case had a hearing threshold of around 50 dB due to a bilateral effusive otitis media. A notable finding was the percentage of children with tubal dysfunction in both evaluations, within 2 months of age and around 12 months of age. Most children revealed normal hearing. The CDI-WG was completed by 56/115 families. The rate of children below the fifth percentile was 8.9% for sentences understood, 12.5% for words understood, and 5.4% for words produced. Concerning CDI-Gestures, only 2 children (3.6%) were below the fifth percentile. A structured audiological follow-up in association with the evaluation of communication skills of children appears fundamental, particularly in the years of maximum neuroplasticity. Long-term studies are still necessary to evaluate the possible consequences of the pandemic.
Personal Goals, User Engagement, and Meal Adherence within a Personalised AI-Based Mobile Application for Nutrition and Physical Activity
Authors: Patra Elena , Kokkinopoulou Anna , Wilson-Barnes Saskia , Hart Kathryn , Gymnopoulos Lazaros P. , Tsatsou Dorothea , Solachidis Vassilios , Dimitropoulos Kosmas , Rouskas Konstantinos , Argiriou Anagnostis , Lalama Elena , Csanalosi Marta , Pfeiffer Andreas F. H. , Cornelissen Véronique , Decorte Elise , Dias Sofia Balula , Oikonomidis Yannis , María Botana José , Leoni Riccardo , Russell Duncan , Mantovani Eugenio , Aleksić Milena , Brkić Boris , Hassapidou Maria , Pagkalos Ioannis
Published: September 2024

Mobile applications have been shown to be an effective and feasible intervention medium for improving healthy food intake in different target groups. As part of the PeRsOnalized nutriTion for hEalthy livINg (PROTEIN) European Union H2020 project, the PROTEIN mobile application was developed as an end-user environment, aiming to facilitate healthier lifestyles through artificial intelligence (AI)-based personalised dietary and physical activity recommendations. Recommendations were generated by an AI advisor for different user groups, combining users’ personal information and preferences with a custom knowledge-based system developed by experts to create personalised, evidence-based nutrition and activity plans. The PROTEIN app was piloted across different user groups in five European countries (Belgium, Germany, Greece, Portugal, and the United Kingdom). Data from the PROTEIN app’s user database (n = 579) and the PROTEIN end-user questionnaire (n = 446) were analysed using the chi-square test of independence to identify associations between personal goals, meal recommendations, and meal adherence among different gender, age, and user groups. The results indicate that weight loss-related goals are more prevalent, as well as more engaging, across all users. Health- and physical activity-related goals are key for increased meal adherence, with further differentiation evident between age and user groups. Congruency between user groups and their respective goals is also important for increased meal adherence. Our study outcomes, and the overall research framework created by the PROTEIN project, can be used to inform the future development of nutrition mobile applications and enable researchers and application designers/developers to better address personalisation for specific user groups, with a focus on user intent, as well as in-app features.
Genome-Wide Identification of the Remorin Gene Family in Poplar and Their Responses to Abiotic Stresses
Authors: Li Zihui , Wang Hang , Li Chuanqi , Liu Huimin , Luo Jie
Published: September 2024

The Remorin (REM) gene family is a plant-specific, oligomeric, filamentous family protein located on the cell membrane, which is important for plant growth and stress responses. In this study, a total of 22 PtREMs were identified in the genome of Populus trichocarpa. Subcellular localization analysis showed that they were predictively distributed in the cell membrane and nucleus. Only five PtREMs members contain both Remorin_C- and Remorin_N-conserved domains, and most of them only contain the Remorin_C domain. A total of 20 gene duplication pairs were found, all of which belonged to fragment duplication. Molecular evolutionary analysis showed the PtREMs have undergone purified selection. Lots of cis-acting elements assigned into categories of plant growth and development, stress response, hormone response and light response were detected in the promoters of PtREMs. PtREMs showed distinct gene expression patterns in response to diverse stress conditions where the mRNA levels of PtREM4.1, PtREM4.2 and PtREM6.11 were induced in most cases. A co-expression network centered by PtREMs was constructed to uncover the possible functions of PtREMs in protein modification, microtube-based movement and hormone signaling. The obtained results shed new light on understanding the roles of PtREMs in coping with environmental stresses in poplar species.