bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the Antibacterial Properties of Iron Oxide, Polyethylene Glycol, and Gentamicin Conjugated Nanoparticles against Some Multidrug-Resistant Bacteria
Authors: Abdulsada Farah M. , Hussein Nehia N. , Sulaiman Ghassan M. , Al Ali Amer , Alhujaily Muhanad
Published: September 2022

Antibacterial resistance is observed as a public health issue around the world. Every day, new resistance mechanisms appear and spread over the world. For that reason, it is imperative to improve the treatment schemes that have been developed to treat infections caused by wound infections, for instance, Staphylococcus epidermidis (S. epidermidis), Proteus mirabilis (P. mirabilis), and Acinetobacter baumannii (A. baumannii). In this case, we proposed a method that involves mixing the Gentamicin (Gen) with iron oxide nanoparticles (Fe3O4 NPs) and a polymer (polyethylene glycol (PEG)) with Fe3O4 NPs. X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), energy dispersive X-ray (EDX), scanning electron microscope (SEM), and transmission electron microscope (TEM) were used to characterize Fe3O4 NPs. Zeta potential and dynamic light scattering (DLS) were also assessed. The antibacterial activity of Fe3O4 NPs, Fe3O4 NPs+PEG, Fe3O4 NPs+Gen, and Fe3O4 NPs+PEG+Gen composites was investigated. The results showed a significant improvement in the antibacterial activity of nanoparticles against bacterial isolates, especially for the Fe3O4 NPs+PEG+Gen as the diameter of the inhibition zone reached 26.33 ± 0.57 mm for A. baumannii, 25.66 ± 0.57 mm for P. mirabilis, and 23.66 ± 0.57 mm for S. epidermidis. The Fe3O4 NPs, Fe3O4 NPs+PEG, Fe3O4+Gen, and Fe3O4+PEG+Gen also showed effectiveness against the biofilm produced by these isolated bacteria. The minimum inhibitory concentration (MIC) of Fe3O4 NPs for S. epidermidis was 25 µg mL−1 and for P. mirabilis and A. baumannii was 50 µg mL−1. The findings suggest that the prepared nanoparticles could be potential therapeutic options for treating wound infections caused by S. epidermidis, P. mirabilis, and A. baumannii.
ZnO/CeO2 Nanocomposites: Metal-Organic Framework-Mediated Synthesis, Characterization, and Estimation of Cellular Toxicity toward Liver Cancer Cells
Authors: Alabyadh Toqa , Albadri Riyadh , Es-haghi Ali , Yazdi Mohammad Ehsan Taghavizadeh , Ajalli Narges , Rahdar Abbas , Thakur Vijay Kumar
Published: September 2022

The Zinc-doped cerium oxide nanocomposite (ZnO/CeO2 NC) was synthesized using a metal-organic framework as a precursor through the combustion method. It was characterized by powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), field emission electron microscopy (FESEM), energy dispersive analysis (EDX), transmission electron microscopy (TEM), dynamic light scattering (DLS), and ξ-potential. The PXRD demonstrated the successful synthesis of ZnO/CeO2 NC with a crystallite size of 31.9 nm. FESEM and TEM images displayed hexagonal and spherical morphologies, and the solid-phase size was 65.03 ± 30.86 nm for ZnO/CeO2 NCs. DLS, TEM, and FESEM showed that the NCs have a high tendency for agglomeration/aggregation in both aqueous media and solid phase. The anticancer attributes of ZnO/CeO2 NC were investigated against Liver cancer cells (HepG2), which showed inhibition of cancer cell growth on a concentration-dependent gradient. The cell toxicity effects of ZnO/CeO2 nanocomposites were also studied toward NIH-3T3, in which the data displayed the lower toxicity of NC compared to the HepG2 cell line.
A Porous Hydrogel with High Mechanical Strength and Biocompatibility for Bone Tissue Engineering
Authors: Xiang Changxin , Zhang Xinyan , Zhang Jianan , Chen Weiyi , Li Xiaona , Wei Xiaochun , Li Pengcui
Published: September 2022

Polyvinyl alcohol (PVA) hydrogels are considered to be ideal materials for tissue engineering due to their high water content, low frictional behavior, and good biocompatibility. However, their limited mechanical properties restrict them from being applied when repairing load-bearing tissue. Inspired by the composition of mussels, we fabricated polyvinyl alcohol/hydroxyapatite/tannic acid (PVA/HA/TA) hydrogels through a facile freeze–thawing method. The resulting composite hydrogels exhibited high moisture content, porous structures, and good mechanical properties. The compressive strength and tensile strength of PVA hydrogels were improved from 0.77 ± 0.11 MPa and 0.08 ± 0.01 MPa to approximately 3.69 ± 0.41 MPa and 0.43 ± 0.01 MPa, respectively, for the PVA/HA/1.5TA hydrogel. The toughness and the compressive elastic modulus of PVA/HA/1.5TA hydrogel also attained 0.86 ± 0.02 MJm−3 and 0.11 ± 0.02 MPa, which was approximately 11 times and 5 times higher than the PVA hydrogel, respectively. The PVA/HA/1.5TA hydrogel also exhibited fatigue resistance abilities. The mechanical properties of the composite hydrogels were improved through the introduction of TA. Furthermore, in vitro PVA/HA/1.5TA hydrogel showed excellent cytocompatibility by promoting cell proliferation in vitro. Scanning electron microscopy analysis indicated that PVA/HA/1.5TA hydrogels provided favorable circumstances for cell adhesion. The aforementioned results also indicate that the composite hydrogels had potential applications in bone tissue engineering, and this study provides a facile method to improve the mechanical properties of PVA hydrogel.
When News Topics Annoy—Exploring Issue Fatigue and Subsequent Information Avoidance and Extended Coping Strategies
Authors: Schumann Christina
Published: September 2022

This paper scrutinizes the phenomenon of issue fatigue and its consequences. Issue fatigue results from overexposure to a news topic that has been on the media’s agenda for an extended period of time. Fatigued recipients become annoyed, and no longer wish to be exposed to the topic. Based on the findings of an explorative qualitative study, a quantitative online survey was conducted in Germany, Mexico, and Pakistan (N = 481). Using cluster analysis, we identified an emotional and a cognitive type of issue fatigue, and investigated how these types react. Both types of fatigued recipients avoided further news about the respective issue in traditional news media (= information avoidance). Differences were observed concerning the strategies to handle fatigue (= coping strategies): recipients of the emotional type posted about their fatigue in social media; recipients of the cognitive type turned to information in sources other than the mainstream news. Taking into account country-specific differences, we concluded that, generally, issue fatigue—via information avoidance—results in an uninformed citizenry. This can be a hurdle for the functioning of an established democracy or for the success of democratic transitions. Posting about issue fatigue, which was more frequent in Mexico and Pakistan, might ‘infect’ others, and intensify problems resulting from issue fatigue. Turning to alternative sources can be either beneficial or problematic for the development of a well-informed citizenry, depending on whether alternative sources provide reliable and truthful information.
Steps towards a Multiple Myeloma Cure?
Authors: Gozzetti Alessandro , Bocchia Monica
Published: September 2022

Multiple myeloma survival has increased in last 20 years because of new treatments, better clinical management due to novel diagnostic tools such as imaging, and better understanding of the disease, biologically and genetically. Novel drugs have been introduced that act with different therapeutic mechanisms, but so have novel therapeutic strategies such as consolidation and maintenance after autologous stem cell transplant. Imaging (such as PET-CT and MRI) has been applied at diagnosis and after therapy for minimal residual disease monitoring. Multiparametric flow and molecular NGS may detect, with high-sensitivity, residual monoclonal plasma cells in the bone marrow. With this novel therapeutic and biological approach, a considerable fraction of multiple myeloma patients can achieve durable remission or even MGUS-like regression, which can ultimately lead to disease disappearance. The big dogma, “Myeloma is an incurable disease”, is hopefully fading.
Effect of General Anesthesia Maintenance with Propofol or Sevoflurane on Fractional Exhaled Nitric Oxide and Eosinophil Blood Count: A Prospective, Single Blind, Randomized, Clinical Study on Patients Undergoing Thyroidectomy
Authors: Vekrakou Artemis , Papacharalampous Panagiota , Logotheti Helena , Valsami Serena , Argyra Eriphyli , Vassileiou Ioannis , Theodoraki Kassiani
Published: September 2022

Background: Nitric oxide (NO) is considered a means of detecting airway hyperresponsiveness, since even non-asthmatic patients experiencing bronchospasm intraoperatively or postoperatively display higher levels of exhaled NO. It can also be used as a non-invasive biomarker of lung inflammation and injury. This prospective, single-blind, randomized study aimed to evaluate the impact of two different anesthesia maintenance techniques on fractional exhaled nitric oxide (FeΝO) in patients without respiratory disease undergoing total thyroidectomy under general anesthesia. Methods: Sixty patients without respiratory disease, atopy or known allergies undergoing total thyroidectomy were randomly allocated to receive either inhalational anesthesia maintenance with sevoflurane at a concentration that maintained Bispectral Index (BIS) values between 40 and 50 intraoperatively or intravenous anesthesia maintenance with propofol 1% targeting the same BIS values. FeΝO was measured immediately preoperatively (baseline), postoperatively in the Postanesthesia Care Unit and at 24 h post-extubation with a portable device. Other variables measured were eosinophil blood count preoperatively and postoperatively and respiratory parameters intraoperatively. Results: Patients in both groups presented lower than baseline values of FeΝO measurements postoperatively, which returned to baseline measurements at 24 h post-extubation. In the peripheral blood, a decrease in the percentage of eosinophils was demonstrated, which was significant only in the propofol group. Respiratory lung mechanics were better maintained in the propofol group as compared to the sevoflurane group. None of the patients suffered intraoperative bronchospasm. Conclusions: Both propofol and sevoflurane lead to the temporary inhibition of NO exhalation. They also seem to attenuate systemic hypersensitivity response by reducing the eosinophil count in the peripheral blood, with propofol displaying a more pronounced effect and ensuring a more favorable mechanical ventilation profile as compared to sevoflurane. The attenuation of NO exhalation by both agents may be one of the underlying mechanisms in the reduction in airway hyperreactivity. The clinical significance of this fluctuation remains to be studied in patients with respiratory disease.
Non-Coding RNAs in Hepatocellular Carcinoma
Authors: Korsch Mascha , Margetts Alexander , Wahlestedt Claes , Lohse Ines
Published: September 2022

Liver cancer ranks as the fourth leading cause of cancer-related deaths. Despite extensive research efforts aiming to evaluate the biological mechanisms underlying hepatocellular carcinoma (HCC) development, little has been translated towards new diagnostic and treatment options for HCC patients. Historically, the focus has been centered on coding RNAs and their respective proteins. However, significant advances in sequencing and RNA detection technologies have shifted the research focus towards non-coding RNAs (ncRNA), as well as their impact on HCC development and progression. A number of studies reported complex post-transcriptional interactions between various ncRNA and coding RNA molecules. These interactions offer insights into the role of ncRNAs in both the known pathways leading to oncogenesis, such as dysregulation of p53, and lesser-known mechanisms, such as small nucleolar RNA methylation. Studies investigating these mechanisms have identified prevalent ncRNA changes in microRNAs, snoRNAs, and long non-coding RNAs that can both pre- and post-translationally regulate key factors in HCC progression. In this review, we present relevant publications describing ncRNAs to summarize the impact of different ncRNA species on liver cancer development and progression and to evaluate recent attempts at clinical translation.
Magneto-Thermoelastic Response in an Infinite Medium with a Spherical Hole in the Context of High Order Time-Derivatives and Triple-Phase-Lag Model
Authors: Allehaibi Ashraf M. , Zenkour Ashraf M.
Published: September 2022

The article presents the interactions of magneto-thermoelastic effects in an isotropic material with a spherical cavity. The spherical cavity is expected to be tractionless and subjected to both heat and magnetic fields. The motion equation contains the Lorentz force. Laplace’s transformation methodology is used with a refined multi-time-derivative triple-phase-lag thermoelasticity theory to develop the generalized magneto-thermoelastic coupled solution. Many results were obtained to serve as benchmarks for future comparisons. The effects of time, magnetic field, and electric permittivity under the thermal environment were investigated.
Multifunctional Hybrid Fiber Composites for Energy Transfer in Future Electric Vehicles
Authors: Adam Till Julian , Wierach Peter , Mertiny Pierre
Published: September 2022

Reducing the weight of electric conductors is an important task in the design of future electric air and ground vehicles. Fully electric aircraft, where high electric energies have to be distributed over significant distances, are a prime example. Multifunctional composite materials with both adequate structural and electrical properties are a promising approach to substituting conventional monofunctional components and achieving considerable mass reductions. In this paper, a hybrid multifunctional glass-fiber-reinforced composite containing quasi-endless aluminum fibers with a diameter of 45 μm is proposed for electric energy transfer. In addition to characterizing the material’s behavior under static and fatigue loads, combined electrical-mechanical tests are conducted to prove the material’s capability of carrying electric current. Light microscopy, thermal imaging and potentiometry-based resistance characterization are used to investigate the damage behavior. It is found that a volume fraction of about 10% work-hardened aluminum fibers does not affect the static fiber-parallel material properties significantly. Under transverse loading, however, the tensile strength is found to decrease by 17% due to the weak bonding of the aluminum fibers. The fiber-parallel fatigue strength of the multifunctional laminate containing work-hardened aluminum fibers is comparable to that of the reference material. In contrast, the integration of soft-annealed aluminum fibers decreases the tensile strength (−10%) and fatigue life (−21%). Concerning the electrical properties, electrical resistance is nearly unchanged until specimen rupture under quasi-static tensile loads, whereas under cyclic loading, it increases up to 60% within the last third of the fatigue life. Furthermore, the material’s capability of carrying currents up to 0.32 A/mm2 (current density of 4.5 A/mm2 in the aluminum phase) is proven. Under combined electrical-mechanical loads, a notable reduction in the fatigue life (−20%) is found at low fatigue loads, which is attributed to ohmic specimen heating. To the best knowledge of the authors, this is the first study on the electrical and mechanical material properties and damage behavior of glass-fiber-reinforced composites containing aluminum fibers tested under combined electrical-mechanical loads.
The Formation Mechanism of Nanocrystals after Martensitic Transformation
Authors: Li Song-Jie , Su Shi-Long , Hu Guan-Jie , Zhao Qiang , Wei Zheng-Yu , Tian Yun , Wang Cheng-Duo , Lu Xing , Ping De-Hai
Published: September 2022

Understanding the ultrafine substructure in freshly formed Fe-C martensite is the key point to reveal the real martensitic transformation mechanism. As-quenched martensite, whose transformation temperature is close to room temperature, has been investigated in detail by means of transmission electron microscopy (TEM) in this study. The observation results revealed that the freshly formed martensite after quenching is actually composed of ultrafine crystallites with a grain size of 1–2 nm. The present observation result matches well with the suggestion based on X-ray studies carried out one hundred years ago. Such nanocrystals are distributed throughout the entire martensite. The whole martensite shows a uniform contrast under both bright and dark field observation modes, irrespective of what observation directions are chosen. No defect contrast can be observed inside each nanocrystal. However, a body-centered cubic {112}<111>-type twinning relationship exists among the ultrafine α-Fe grains. Such ultrafine α-Fe grains or crystallites are the root cause of the fine microstructure formed in martensitic steels and high hardness after martensitic transformation. The formation mechanism of the ultrafine α-Fe grains in the freshly formed martensite will be discussed based on a new γ → α phase transformation mechanism.
Mechanical Properties and Uniaxial Failure Behavior of Concrete with Different Solid Waste Coarse Aggregates
Authors: Zhou Mei , Bai Jinting , Li Shaowei , Zhang Kai , Li Chao , Wang Xinyi
Published: September 2022

To reveal the differences between the mechanical properties of solid waste coarse aggregate concrete and natural coarse aggregate concrete (NCAC) under equal strength, the basic mechanical properties of coarse aggregate concrete with seven different solid wastes (i.e., self-combusted coal gangue, uncombusted coal gangue, marble sheet waste, granite sheet waste, iron waste rock, recycled concrete, and self-combusted coal gangue ceramicite) were tested, and the trends in failure morphology, elastic modulus, and the stress–strain full curves of the different solid waste coarse aggregate concretes were analyzed and compared with NCAC. Finally, the interfacial structure of the concrete was characterized by SEM. The results showed that C30 strength grade concrete was prepared with different solid waste coarse aggregates; however, the 28 d compressive strength, split tensile strength, axial compression strength, flexural strength, and elastic modulus of the concrete was 35.26–47.35, 2.13–3.35, 26.43–42.70, 2.83–3.94, and 17.3–31.2, respectively. The modulus of elasticity of the solid waste coarse aggregate concrete was smaller than the NCAC under equal strength, with a maximum difference of 45%. The peak compressive strain and ultimate compressive strain were larger than the NCAC, with a maximum difference of 43%. The crushing value of the solid waste coarse aggregate affected the splitting tensile strength, flexural strength, and modulus of elasticity of the concrete to a greater extent than the compressive strength. The transition zone at the concrete interface of the coarse aggregates with different solid wastes varied widely. The porous micro-pumping effect of the self-combusted gangue and self-combusted gangue vitrified reinforced the concrete interface transition zone, and the polished surface of sheet waste, uncombusted gangue, and recycled concrete aggregate surface adhesion weakened the interface transition zone; Finally, the uniaxial compressive stress–strain curve model for concrete with different solid waste coarse aggregates was established based on the Guo Zhenhai model.
High-Efficiency Iron Extraction from Low-Grade Siderite via a Conveyor Bed Magnetization Roasting–Magnetic Separation Process: Kinetics Research and Applications
Authors: Jiu Shaowu , Zhao Bo , Yang Chao , Chen Yanxin , Cheng Fuan
Published: September 2022

Upgrading and utilizing low-grade iron ore is of great practical importance to improve the strategic security of the iron ore resource supply. In this study, a thermal analysis–infrared (IR) analysis–in-situ IR method was used to investigate the reaction mechanism and kinetics of Daxigou siderite. Experiments were conducted using a conveyor bed magnetization roasting process (CBMRP) to investigate the magnetization of siderite. Multi-stage magnetic separation processes were adopted to extract magnetite. The results show that simultaneously the iron carbonate in siderite decomposes, and magnetite is formed between 364 °C and 590 °C under both inert and reducing atmospheres. The activation energy of the magnetization roasting reaction is 106.1 kJ/mol, consistent with a random nucleation and growth reaction mechanism. Magnetization roasting at 750–780 °C for approximately 3.5 s in the CBMRP results in a magnetic conversion rate of >0.99 of the iron minerals in the siderite. A beneficiation process of one roughing, one sweeping, and three cleaning processes was adopted. A dissociation particle size of −400 mesh accounting for 94.78%, a concentrate iron grade of 62.8 wt.%, and a recovery of 68.83% can be obtained. Overall, a theoretical and experimental basis is presented for the comprehensive utilization of low-grade siderite.
Magnetic Properties of GaAs/NiFe Coaxial Core-Shell Structures
Authors: Monaico Eduard V. , Morari Vadim , Kutuzau Maksim , Ursaki Veaceslav V. , Nielsch Kornelius , Tiginyanu Ion M.
Published: September 2022

Uniform nanogranular NiFe layers with Ni contents of 65%, 80%, and 100% have been electroplated in the potentiostatic deposition mode on both planar substrates and arrays of nanowires prepared by the anodization of GaAs substrates. The fabricated planar and coaxial core-shell ferromagnetic structures have been investigated by means of scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM). To determine the perspectives for applications, a comparative analysis of magnetic properties, in terms of the saturation and remanence moment, the squareness ratio, and the coercivity, was performed for structures with different Ni contents.
Application of the Generalized Method of Moving Coordinates to Calculating Stress Fields near an Elliptical Hole
Authors: Alexandrov Sergei , Rynkovskaya Marina , Tsai Shang-Nan
Published: September 2022

The distribution of stresses near holes is of great importance in fracture mechanics and material modeling. The present paper provides a general stress solution near a traction-free surface for an arbitrary piecewise linear yield criterion, assuming plane-strain conditions. The generalized method of moving coordinates is proven efficient in this case. In particular, the solution reduces to evaluating one ordinary integral. The boundary value problem solved is a Cauchy problem for a hyperbolic system of equations. Therefore, the stress solution in the plastic region is independent of other boundary conditions, though the occurrence of plastic yielding at a specific point is path-dependent. The general solution applies to calculating the stress field near an elliptic hole. It is shown that the parameter that controls the pressure-dependency of the yield criterion affects the stress field significantly. The aspect ratio is less significant as compared to that parameter. However, for a given material, the aspect ratio should also be considered to predict the stress field accurately, especially in the near vicinity of the hole. The solution reduces to an available solution for the pressure-independent yield criterion, which is a particular yield criterion of the considered class of yield criteria.
Microstructural Characteristics and Hardness Enhancement of Super Duplex Stainless Steel by Friction Stir Processing
Authors: Pan Linlin , Kwok Chi Tat , Lo Kin Ho
Published: September 2022

In the present study, microstructural evolution and hardness of the friction stir processed (FSPed) SAF 2507 super duplex stainless steel fabricated at a rotational speed of 650 rpm and a traverse speed of 60 mm/min were investigated. A scanning electron microscope (SEM) equipped with an electron backscatter diffraction (EBSD) detector was used to study the microstructure of the stir zone. The grain sizes of austenite and ferrite in the FSPed 2507 were found to be smaller (0.75 and 0.96 μm) than those of the substrate (6.6 and 5.6 μm) attributed to the occurrence of continuous dynamic recrystallization (CDRX) in both phases. Higher degree of grain refinement and DRX were obtained at the advancing side of the FSPed specimens due to higher strain and temperature. A non-uniform hardness distribution was observed along the longitudinal direction of the SZ. The maximum hardness was obtained at the bottom (407 HV1).
A Novel Method of Flow Curve Measurement for Magnetic Fluid Based on Plane Poiseuille Flow
Authors: Dong Jiahao , Hu Yifan , Su Bingrui , Li Zhenkun , Song Zhongru , Li Decai , Cui Hongchao , Wang Deyi
Published: September 2022

Accurate measurement of the flow curves of magnetic fluid under a uniform field has always been a challenge. In this article, a novel method is proposed to measure the flow curve of magnetic fluids based on plane Poiseuille flow. The measuring system was built and its performance was compared with that of a commercial rheometer. Flow curves of magnetic fluid with different zero-field viscosity were tested under various field strengths. This novel method facilitates direct observation of the flowing behaviors of magnetic fluid under different stresses. By examining the variation trend of viscosity under certain constant stress, a more reliable method to determine the dynamic yield stress of magnetic fluid was used. The dynamic yield stress of the magnetic fluid measured by the new method was larger than the value obtained by the fitting, which is more reliable from an engineering point of view.
Free–Free Beam Resting on Tensionless Elastic Foundation Subjected to Patch Load
Authors: Musa Abubakr E. S. , Al-Shugaa Madyan A. , Al-Fakih Amin
Published: September 2022

Despite the popularity of a completely free beam resting on a tensionless foundation in the construction industry, the existing bending analysis solutions are limited to certain types of loads (mostly point and uniformly distributed loads); these are also quite complex for practicing engineers to handle. To overcome the associated complexity, a simple iterative procedure is developed in this study, which uses the Ritz method for the bending analysis of a free–free beam on a tensionless foundation subjected to a patched load. The Ritz method formulation is first presented with polynomials being used to approximate the beam deflection with unknown constants to be determined through minimization of the potential energy. To account for the tensionless action, the subgrade reaction is set to zero when the deflection is negative. The non-zero subgrade reaction zone is defined by αlL/2<x<αrL/2 where the coefficients αl and αr are to be determined iteratively. A numerical example is presented to illustrate the applicability of the proposed procedure for symmetrical and asymmetrical problems. The obtained results show high negative deflection, which proves the occurrence of separation between the beam and the supporting tensionless foundation. This location of negative deflection is called the lifted zone, while the point that separates between the negative and positive deflection is called the lift-off point. A parametric study is then performed to study the effect of the amount of load, stiffness of the beam, and the subgrade reaction on the length of the lifted zone. The results of the parametric study indicate that for the same beam stiffness to subgrade reaction modulus ratio (EI/k), the lift-off point remains the same and beams with lower stiffnesses or higher loads deflect more.
Data Depth and Multiple Output Regression, the Distorted M-Quantiles Approach
Authors: Ochoa Maicol , Cascos Ignacio
Published: September 2022

For a univariate distribution, its M-quantiles are obtained as solutions to asymmetric minimization problems dealing with the distance of a random variable to a fixed point. The asymmetry refers to the different weights awarded to the values of the random variable at either side of the fixed point. We focus on M-quantiles whose associated losses are given in terms of a power. In this setting, the classical quantiles are obtained for the first power, while the expectiles correspond to quadratic losses. The M-quantiles considered here are computed over distorted distributions, which allows to tune the weight awarded to the more central or peripheral parts of the distribution. These distorted M-quantiles are used in the multivariate setting to introduce novel families of central regions and their associated depth functions, which are further extended to the multiple output regression setting in the form of conditional and regression regions and conditional depths.
A Nutraceutical Formulation Containing Brown Algae Reduces Hepatic Lipid Accumulation by Modulating Lipid Metabolism and Inflammation in Experimental Models of NAFLD and NASH
Authors: Gabbia Daniela , Roverso Marco , Zanotto Ilaria , Colognesi Martina , Sayaf Katia , Sarcognato Samantha , Arcidiacono Diletta , Zaramella Alice , Realdon Stefano , Ferri Nicola , Guido Maria , Russo Francesco Paolo , Bogialli Sara , Carrara Maria , De Martin Sara
Published: September 2022

Recently, some preclinical and clinical studies have demonstrated the ability of brown seaweeds in reducing the risk factors for metabolic syndrome. Here, we analyzed the beneficial effect of a nutraceutical formulation containing a phytocomplex extracted from seaweeds and chromium picolinate in animal models of liver steatosis of differing severities (rats with non-alcoholic fatty liver disease (NAFLD) and its complication, non-alcoholic steatohepatitis (NASH)). This treatment led to a significant drop in hepatic fat deposition in both models (p < 0.01 vs. untreated animals), accompanied by a reduction in plasma inflammatory cytokines, such as interleukin 6, tumor necrosis factor α, and C reactive protein, and myeloperoxidase expression in liver tissue. Furthermore, a modulation of the molecular pathways involved in lipid metabolism and storage was demonstrated, since we observed the significant reduction of the mRNA levels of fatty acid synthase, diacylglycerol acyltransferases, the sterol-binding protein SREBP-1, and the lipid transporter perilipin-2, in both treated NAFLD and NASH rats in comparison to untreated ones. In conclusion, this nutraceutical product was effective in reducing liver steatosis and showed further beneficial effects on hepatic inflammation and glycemic control, which were particularly evident in rats characterized by a more severe condition, thus representing a therapeutic option for the treatment of NAFLD and NASH patients.
Cystitis Induces Altered CREB Expression Related with Micturition Reflex
Authors: Choi Taesoo , Lee Dong-Gi
Published: September 2022

Background and objectives: Bladder stimulation upregulates neurotrophins associated with voiding reflex. Bacterial cystitis can be a stimulant that activates this system, resulting in a pathological state. Phosphorylated responsive element of binding protein (p-CREB) is a pivotal transcriptional factor in the neurotrophin signaling cascade. The goal of our study was to examine the change in expression of p-CREB in dorsal root ganglia (DRG) of rats after uropathogenic Escherichia coli infection of the bladder. Materials and methods: A total of 19 adult female Sprague–Dawley rats were induced with acute E. coli infection (n = 7), chronic E. coli infection (n = 6), or served as controls (n = 6). In each group, the profiles of p-CREB cell were counted in 6–10 sections of each of the DRG collected. DRG cells exhibiting intense nuclear staining were considered to be positive for p-CREB immunoreactivity (p-CREB-IR). Results: Overall, the immunoreactivity of p-CREB was examined in smaller cell profiles with nuclear staining or nuclear and cytoplasmic staining in the DRGs (L1–L6, S1). In the chronic cystitis group, p-CREB-IR in the L1–L6 and S1 DRG was significantly higher than the control group (p < 0.05). Further, p-CREB-IR in the L3–L6 and S1 DRG of the chronic cystitis group was significantly greater than that in the acute cystitis group (p < 0.05). In the control and acute cystitis groups, p-CREB-IR in the L4–L5 DRG was significantly lower than that found in the other DRG sections (p < 0.05). Conclusions: Altogether, acute or chronic E.coli cystitis changed the immunoreactivity of p-CREB in lumbosacral DRG cells. In particular, chronic E. coli infection triggered p-CREB overexpression in L1–L6 and S1 DRG, indicating subsequent pathologic changes.
Accuracy of the Tibial Component Alignment by Extramedullary System Using Simple Radiographic References in Total Knee Arthroplasty
Authors: Cho Jin-Ho , Choi Jun Young , Lee Sung-Sahn
Published: September 2022

Background and Objectives: The tibial component alignment is an important issue for the longevity of total knee arthroplasty (TKA). The purpose of our study was to investigate the usefulness of proximal tibial references determined by pre-operative radiography and intraoperative C-arm-guided hip and ankle center marking for the extramedullary guided tibial cut in mild (<10°) and severe (≥10°) varus knee TKA. Materials and Methods: A total of 150 consecutive patients (220 cases) who underwent total knee arthroplasty who were recruited from July 2011 to April 2017 were reviewed retrospectively. Before surgery, the proximal tibial reference point and medio-lateral cut thickness difference were identified. Then, hip and ankle centers were checked using a C-arm intensifier intraoperatively. The hip–knee–ankle (HKA) alignment and medial proximal tibial angle (MPTA) were assessed pre-operatively and post-operatively. More than 3° varus or valgus of HKA alignment or tibial component angle was defined as an outlier. Results: Mean follow-up duration was 26.9 months. Among 220 cases, 111 cases are classified as mild varus group and 109 cases are classified as severe varus group. The HKA alignment is significantly improved (p < 0.001). The average tibial component angle after surgery is 90.1°. A total of 21 cases (9.5%) and 3 cases (1.4%) are classified as outliers of HKA alignment and MPTA, respectively. Among MPTA outliers, one case is in the mild varus group and two cases are in the in severe varus group (p = 0.62). Conclusion: Measurement of proximal tibial radiographic references and checking the C-arm-guided intraoperative hip and ankle center could be helpful to obtain the favorable coronal position of the tibial component in the extramedullary guided tibial cut.
Vertical Control of a Severe Hyperdivergent Skeletal Class II Malocclusion with Steep Posterior Occlusal Plane in a Camouflage Case
Authors: Lu Yun , Zhang Weihua , Zhao Bingjiao , Liu Yuehua
Published: September 2022

Severe hyperdivergent skeletal Class II malocclusion may be ideally treated with orthognathic surgery in adult patients. Here, we report a camouflage treatment of a 23-year-old female patient. She was diagnosed with a skeletal Class II malocclusion with extreme high mandibular plane angle, retrusive mandible, steep posterior occlusal plane, anterior open bite, and severe overjet. The treatment plan included extraction of all second premolars and intrusion of the maxillary anterior teeth and mandibular posterior teeth using miniscrews. These contributed to an effective counterclockwise rotation of the mandible, decreased lower face height, and improvement in anterior overbite. This case report shows a vertical control strategy on severe hyperdivergent skeletal Class II malocclusions, which achieves well-controlled sagittal and vertical dimensions and a favorable facial appearance. The treatment and retention results were well balanced and aesthetically pleasing.
Effects of McConnell and Kinesio Tapings on Pain and Gait Parameters during Stair Ambulation in Patients with Patellofemoral Pain Syndrome
Authors: Yoon Samwon , Son Hohee
Published: September 2022

Background and Objectives: The purpose of this study was to investigate the effects of McConnell and Kinesio tapings on knee pain and gait parameters during stair ambulation in patients with patellofemoral pain syndrome (PFPS). Materials and Methods: We selected 52 young adults suffering from anterior knee pain due to PFPS to participate. Then, we randomly assigned 26 patients to either the McConnell or the Kinesio taping groups. We measured their knee pain and gait parameters during stair ambulation before and after the interventions. For the measured data, we performed a paired t-test to evaluate the amount of change before and after the intervention within the groups and an independent t-test to compare the groups. Results: From the comparison within the groups, we found a significant difference in both groups in the anterior knee pain scale score (p < 0.05) and a significant difference between the groups as well (p < 0.05). As a result of the analysis of the gait parameters while ascending stairs in the comparison within the groups, both groups showed significant differences in all gait variables, except for the double-support stance (p < 0.05), and we found significant differences in all gait variables, except for the double-support stance, in the comparison between the groups (p < 0.05). Regarding the gait variables during stair descent in the comparison within the groups (p < 0.05), both groups showed significant differences in all of the gait variables; we noted significant differences in the double-support stance, step length, velocity, and cadence in the comparison between the groups (p < 0.05). Conclusions: The McConnell and Kinesio tapings were effective in improving knee pain and gait parameters during ambulation in patients with PFPS, but we found that the McConnell taping had a significant impact on pain reduction during stair ambulation, resulting in further improvement in the gait variables.
Idiopathic Multicentric Castleman Disease with Cutaneous Manifestation: Case Report
Authors: Kosmidis Christoforos S. , Mystakidou Chrysi Maria , Koimtzis Georgios , Papadopoulou Evanthia , Theodorou Vasiliki , Katsios Nikolaos Iason , Georgakoudi Eleni , Sevva Christina , Charalampous Ioannis , Varsamis Nikolaos , Koulouris Charilaos , Michael Christina , Papadopoulos Konstantinos , Anthimidis Georgios , Baka Sofia
Published: September 2022

Castleman disease constitutes a rare class of lymphoproliferative disorders, with an estimated incidence of 21 to 25 per million patient years. The idiopathic subtype exhibits a significantly diverse clinical presentation, which can imitate many autoimmune, malignant, and infectious diseases. Cutaneous manifestations are uncommon and require in-depth investigation, especially when concurrent lymphadenopathy is present. A 79-year-old female, with a chronic, complicated erysipelas-like lesion, presented with bilaterally enlarged inguinal lymph nodes; after surgical excision, their histopathological examination revealed Castleman disease. Even though it is a benign condition, patients are often predisposed to developing certain types of malignancies, which can deteriorate their prognosis. An accurate and early diagnosis, along with effective treatment and prevention of recurrence, is of utmost importance in order to increase the patients’ overall survival and quality of life.
Optimal Release Timing of Drain Clamping to Reduce Postoperative Bleeding after Total Knee Arthroplasty with Intraarticular Injection of Tranexamic Acid
Authors: Kim Myung-Ku , Ko Sang-Hyun , Nam Yoon-Cheol , Jeon Yoon-Sang , Kwon Dae-Gyu , Ryu Dong-Jin
Published: September 2022

Background and Objectives: Intraarticular injection of tranexamic acid (IA-TXA) plus drain-clamping is a preferred method of reducing bleeding after total knee arthroplasty (TKA). However, no consensus has been reached regarding the timing of the clamping. The purpose of this study was to determine the optimum duration of drain-clamping after TKA with IA-TXA. Materials and Methods: We retrospectively reviewed 151 patients that underwent unilateral TKA with IA-TXA plus drain-clamping for 30 min, 2 h, or 3 h. The total drained volume was reviewed as the primary outcome, and hematocrit (Hct) reductions, estimated blood loss (EBL), transfusion rates, and wound complications were reviewed as secondary outcomes. Results: The mean total drained volume, Hct reduction, and EBL were significantly less in the 3 h group than in the 30 min group. Between the 2 h and 3 h groups, there was no statistical difference in the mean total drained volume, Hct reduction, or EBL. The proportion of patients who drained lesser than 300 mL was high in the 3 h group. No significant intergroup difference was observed for transfusion volume, transfusion rate, and wound related complications. Conclusions: In comparison of the IA-TXA plus drain-clamping after TKA, there was no difference in EBL between the 2 h group and the 3 h group, but the amount of drainage volume was small in the 3 h group.
A Comparative Analysis of Dental Measurements in Physical and Digital Orthodontic Case Study Models
Authors: Baciu Elena-Raluca , Budală Dana Gabriela , Vasluianu Roxana-Ionela , Lupu Costin Iulian , Murariu Alice , Gelețu Gabriela Luminița , Zetu Irina Nicoleta , Diaconu-Popa Diana , Tatarciuc Monica , Nichitean Giorgio , Luchian Ionuț
Published: September 2022

Background and Objectives: Study models are essential tools used in the dental teaching process. The aim of the present study was to compare the values obtained by manual and digital orthodontic measurements on physical and digital case study models. Materials and Methods: The physical experimental models were obtained by traditional pouring (improved stone-type IV gypsum products) and by additive manufacturing (resins). The digital experimental models were created by scanning the physical ones, using a white light-emitting diode (LED) source and an L-shaped dental scanner—Swing DOF (DOF, Seoul, Korea). The physical study models were first measured using a digital caliper, and then, they were scanned and evaluated using the DentalCad 3.0 Galway software (exocad GmbH, Darmstadt, Germany). The Pont, Linder–Harth, and Bolton indices, which are used in orthodontics for training students, were derived using the available data. Results: When comparing the linear measurement mean ranks taken on physical study models to those of digital models, no statistically significant differences (p > 0.05) were found. A similar result was also shown when the dentoalveolar growth indicators were analyzed. Conclusions: It can be concluded that dental study models made by direct light processing (DLP) and pouring type IV class gypsum are both acceptable for orthodontic teaching purposes.
Biomechanical Role of Epsin in Influenza A Virus Entry
Authors: Joseph Jophin G. , Mudgal Rajat , Lin Shan-Shan , Ono Akira , Liu Allen P.
Published: September 2022

Influenza A virus (IAV) utilizes clathrin-mediated endocytosis for cellular entry. Membrane-bending protein epsin is a cargo-specific adaptor for IAV entry. Epsin interacts with ubiquitinated surface receptors bound to IAVs via its ubiquitin interacting motifs (UIMs). Recently, epsin was shown to have membrane tension sensitivity via its amphiphilic H0 helix. We hypothesize this feature is important as IAV membrane binding would bend the membrane and clinical isolates of IAVs contain filamentous IAVs that may involve more membrane bending. However, it is not known if IAV internalization might also depend on epsin’s H0 helix. We found that CALM, a structurally similar protein to epsin lacking UIMs shows weaker recruitment to IAV-containing clathrin-coated structures (CCSs) compared to epsin. Removal of the ENTH domain of epsin containing the N-terminus H0 helix, which detects changes in membrane curvature and membrane tension, or mutations in the ENTH domain preventing the formation of H0 helix reduce the ability of epsin to be recruited to IAV-containing CCSs, thereby reducing the internalization of spherical IAVs. However, internalization of IAVs competent in filamentous particle formation is not affected by the inhibition of H0 helix formation in the ENTH domain of epsin. Together, these findings support the hypothesis that epsin plays a biomechanical role in IAV entry.
Xylem-Inspired Hydrous Manganese Dioxide/Aluminum Oxide/Polyethersulfone Mixed Matrix Membrane for Oily Wastewater Treatment
Authors: Yun Teng Sam , Oh Pei Ching , Toh Moau Jian , Yap Yun Kee , Te Qin Yi
Published: September 2022

Ultrafiltration membrane has been widely used for oily wastewater treatment application attributed to its cost-efficiency, ease of operation, and high separation performance. To achieve high membrane flux, the pores of the membrane need to be wetted, which can be attained by using hydrophilic membrane. Nevertheless, conventional hydrophilic membrane suffered from inhomogeneous dispersion of nanofillers, causing a bottleneck in the membrane flux performance. This called for the need to enhance the dispersion of nanofillers within the polymeric matrix. In this work, in-house-fabricated hydrous manganese dioxide–aluminum oxide (HMO-Al2O3) was added into polyethersulfone (PES) dope solution to enhance the membrane flux through a xylem-inspired water transport mechanism on capillary action aided by cohesion force. Binary fillers HMO-Al2O3 loading was optimized at 0.5:0.5 in achieving 169 nm membrane mean pore size. Membrane morphology confirmed the formation of macro-void in membrane structure, and this was probably caused by the hydrophilic nanofiller interfacial stress released in PES matrix during the phase inversion process. The superhydrophilic properties of PES 3 in achieving 0° water contact angle was supported by the energy-dispersive X-ray analysis, where it achieved high O element, Mn element, and Al elements of 39.68%, 0.94%, and 5.35%, respectively, indicating that the nanofillers were more homogeneously dispersed in PES matrix. The superhydrophilic property of PES 3 was further supported by high pure water flux at 245.95 L/m2.h.bar, which was 3428.70% higher than the pristine PES membrane, 197.1% higher than PES 1 incorporated with HMO nanofiller, and 854.00% higher than PES 5 incorporated with Al2O3 nanofillers. Moreover, the excellent membrane separation performance of PES 3 was achieved without compromising the oil rejection capability (98.27% rejection) with 12 g/L (12,000 ppm) oily wastewater.
Improved Compressive Properties of Lattice Structure Based on an Implicit Surface Hybrid Optimization Design Method via Selective Laser Melting
Authors: Xiao Xiong , Xie Liangwen , Tang Rongyao , Liu Jiaan , Song Peng , Zhu Xianyong , Zhao Jiali , Jiang Cheng , Yang Song , Wu Peng
Published: September 2022

In recent years, the lattice structure produced by additive manufacturing is a type of metal foam that has been increasingly investigated for its unique mechanical properties. However, the conventional Computer-Aided Design (CAD) is inefficient, the triply periodic minimal surfaces are rarely mixed, and the smooth transitions at the boundaries are not considered. In this study, a hybrid optimization design method based on implicit surfaces is proposed, which combines multiple implicit surfaces to achieve the continuous change in the curvature at the structure junctions and reduce the stress concentration. The hybrid lattice structures designed by this method were additively manufactured using 316L alloy via a selective laser melting. The results of the finite element analysis and mechanical compression test show that the hybrid lattice structures generated by this method exhibit a higher yield strength and energy absorption. These works can be used for other implicit surfaces, improve and enrich the types of implicit surfaces, and provide more good choices for practical applications.