bluuebunny/crossref_metadata_2025 huggingface dataset:
Sustainable Use of Reservoir Sediment through Partial Application in Building Material
Authors: Junakova Natalia , Junak Jozef
Published: May 2017

Sediment, often considered a by-product of various activities within river basin management to be disposed of, or a pollutant to be controlled, is increasingly being acknowledged as a resource in need of management. The paper deals with the possibility of reusing sediment from two Slovak reservoirs (Klusov and Ruzin) as an alternative raw material in concrete production. Concrete specimens were prepared by a combination of original reservoir sediment, reservoir sediment mechanically activated by dry milling, reservoir sediment mechanically activated by dry milling together with biomass incinerator fly-ash as a binder. To improve the strength properties of specimens, sodium hydroxide (NaOH) was used as a sediment activator. Mixtures containing 40% of binder replacement by the above-mentioned combinations of original and treated sediments were tested for flexural and compressive strengths after 28, 90 and 365 days of curing. The results showed that the mixtures prepared from sediments milled without and with addition of fly ash as cement replacement satisfied the strength requirements for the compressive strength class C16/20 according to the European standard except the composites prepared with NaOH as the sediment activator. Addition of NaOH into composites in the concentration of 5 M as an activator of sediment indicated the negative impact on compressive and flexural strengths and thus NaOH was not an effective pozzolanic activator for sediments. This study reveals that the sediment may be considered as 40% cement substitution in building materials.
Sustainable Environmental Management Indicators in South African Primary Schools
Authors: De Sousa Luiza , Richter Barry , Raath Schalk
Published: May 2017

This research explores sustainable environmental management indicators in South African primary schools. Of key interest is the comparison of a township, farm and urban primary school that identify indicators that promote education for sustainable development in schools that implement an environmental management system. Data are drawn from one-on-one interviews, focus group interviews, observations and document analysis from 35 participants in three schools. A comparison of the three schools was done by content and thematic analysis of a within-case analysis. Data from the township school revealed that socioeconomic factors and organisational structure promote education for sustainable development. The farm school data revealed that health promotion can be managed within an environmental management system within a hierarchical school structure. The urban school data revealed that an economic inducement brings a school to realise that it can reduce its carbon footprint, gain financially and utilize its resources with innovation. A case is made that the four pillars of sustainable development (environment, society, economy, and governance) endorse education for sustainable development. Furthermore, the objectives of environmental education ought to remain nested in an environmental management system to ensure that the global goal of quality education is achieved.
Food Image Recognition via Superpixel Based Low-Level and Mid-Level Distance Coding for Smart Home Applications
Authors: Zheng Jiannan , Wang Z. , Zhu Chunsheng
Published: May 2017

Food image recognition is a key enabler for many smart home applications such as smart kitchen and smart personal nutrition log. In order to improve living experience and life quality, smart home systems collect valuable insights of users’ preferences, nutrition intake and health conditions via accurate and robust food image recognition. In addition, efficiency is also a major concern since many smart home applications are deployed on mobile devices where high-end GPUs are not available. In this paper, we investigate compact and efficient food image recognition methods, namely low-level and mid-level approaches. Considering the real application scenario where only limited and noisy data are available, we first proposed a superpixel based Linear Distance Coding (LDC) framework where distinctive low-level food image features are extracted to improve performance. On a challenging small food image dataset where only 12 training images are available per category, our framework has shown superior performance in both accuracy and robustness. In addition, to better model deformable food part distribution, we extend LDC’s feature-to-class distance idea and propose a mid-level superpixel food parts-to-class distance mining framework. The proposed framework show superior performance on a benchmark food image datasets compared to other low-level and mid-level approaches in the literature.
Lifting the Information Barriers to Address Sustainability Challenges with Data from Physical Geography and Earth Observation
Authors: Lehmann Anthony , Chaplin-Kramer Rebecca , Lacayo Martin , Giuliani Grégory , Thau David , Koy Kevin , Goldberg Grace , Jr. Richard Sharp
Published: May 2017

Sustainability challenges demand solutions, and the pace of technological and scientific advances in physical geography and Earth observation have great potential to provide the information needed to address these challenges. This paper highlights five online tools and initiatives that are lifting barriers to address these challenges. The enviroGRIDS project in the Black Sea catchment demonstrates how the use of spatial data infrastructures can facilitate data sharing. Google Earth Engine is providing solutions to challenges of processing big data into usable information. Additionally, application programming interfaces allow outsiders to elaborate and iterate on programs to explore novel uses of data and models, as seen in the Berkeley Ecoinformatics Engine. Finally, collaborative mapping tools, such as Seasketch/MarineMap and the InVEST software suite, allow engagement within and between groups of experts and stakeholders for the development, deployment, and long-term impact of a project. Merging these different experiences can set a new standard for online information tools supporting sustainable development from evidence brought by physical geography combined with socioeconomic conditions.
Research on the Coupling Coordination of a Sea–Land System Based on an Integrated Approach and New Evaluation Index System: A Case Study in Hainan Province, China
Authors: Zhao Liming , Li Ling , Wu Yujie
Published: May 2017

Based on the concept of sea–land coordination and the statistical data of Hainan Province from 1999 to 2013, we establish a new evaluation index system included four aspects—economic development, social progress, environmental protection and resource efficiency—and use the integrated approach (such as the combination weight method, the coupling coordination degree model, the scissors difference model and the dynamic coupling coordination degree model) to measure the coupling coordination degree of a sea–land system. The results show that: (1) the overall development level of sea system and land system are gradually improved; (2) the coupling coordination degree of sea–land system is gradually from moderately uncoordinated to well coordinated, and the comprehensive evaluation value of sea system has a greater effect on the coupling coordination degree than that of land system; (3) the scissors difference between sea system and land system is gradually increasing; (4) the dynamic coupling coordination degree of the sea–land system which favors a parabolic shape is basically in the break-in development stage; (5) in the process of sea–land system coordination, the influencing factors of economic development, the social progress and resource efficiency should be given priority and, at the same time, strengthen the environmental protection efforts and awareness to promote the role of environmental protection in the sea–land coordination.
A New Sustainability City Index Based on Intellectual Capital Approach
Authors: Alfaro-Navarro José-Luis , López-Ruiz Víctor-Raúl , Peña Domingo Nevado
Published: May 2017

Urban sustainability is a key factor that must be considered at the local level, however, there are few studies that consider sustainability using the triple bottom line approach and apply it to a large number of cities. In this paper, we develop a sustainability city index based on the triple bottom line using an intellectual capital approach that attempts to solve the negative aspects identified in the main indices proposed in the existing literature, such as the use of: subjective weightings, an arithmetic average or index that is not comparable. Here, we have used information available in the Urban Audit database for 2009. The results for 158 cities in 24 European countries show that the cities with the best positions are in the northern European countries. German cities occupied the best positions in the three dimensions of sustainability, albeit with a slightly worse performance in the social dimension. Moreover, the proposal index is consistent, without redundancy among the variables considered in the three dimensions.
A Day-Ahead Wind Power Scenario Generation, Reduction, and Quality Test Tool
Authors: Yıldız Ceyhun , Tekin Mustafa , Gani Ahmet , Keçecioğlu Ö. , Açıkgöz Hakan , Şekkeli Mustafa
Published: May 2017

During the last decades, thanks to supportive policies of countries and a decrease in installation costs, total installed capacity of wind power has increased rapidly all around the world. The uncertain and variable nature of wind power has been a problem for transmission system operators and wind power plant owners. To solve this problem, numerous wind power forecast systems have been developed. Unfortunately none of them can obtain absolutely accurate forecasts yet. Thus, researchers assumed that wind power generation is a stochastic process and they proposed a stochastic programming approach to solve problems arising from the uncertainty of wind power. It is well known that representing stochastic process by possible scenarios is a major issue in the stochastic programming approach. Large numbers of scenarios can represent a stochastic process accurately, but it is not easy to solve a stochastic problem that contains a large number of scenarios. For this reason scenario reduction methods have been introduced. Finally, the quality of this reduced scenario set must be at an acceptable level to use them in calculations. All of these reasons have encouraged authors to develop a wind power scenario tool that can generate and reduce the scenario set and test the quality of it. The developed tool uses historical data to model wind forecast errors. Scenarios are generated around 24 day-ahead point wind power forecasts. A fast forward reduction algorithm is used to reduce the scenario set. Two metrics are proposed to assess the quality of the reduced scenario set. Site measurements are used to test the developed wind power scenario tool. Results showed that the tool can generate and reduce the scenario set successfully and the proposed metrics are useful to assess the quality.
The Role of Technology Spillovers in the Process of Water Pollution Abatement for Large International Firms
Authors: Aldieri Luigi , Vinci Concetto
Published: May 2017

The objective of this paper is to analyze the effects of technology externalities stemming from different technological sectors for international firms engaged both in water pollution abatement and in dirty activities. We present a theoretical framework and an empirical analysis based upon a dataset composed of worldwide R&D-intensive firms. In order to identify the technological proximity between the firms, we construct an original Mahalanobis environmental industry weight matrix, based on the construction of technological vectors for each firm, with European ecological patents distributed across more technology classes. Opportune econometric techniques that deal with the firms’ unobserved heterogeneity and the weak exogeneity of the explanatory variables are implemented. The findings show significant spillover effects on the productivity and environmental performance of the firms.
Realities, Perceptions, Challenges and Aspirations of Rural Youth in Dryland Agriculture in the Midelt Province, Morocco
Authors: Giuliani Alessandra , Mengel Sebastian , Paisley Courtney , Perkins Nicole , Flink Ingrid , Oliveros Oliver , Wongtschowski Mariana
Published: May 2017

Active involvement of youth in agriculture is necessary for sustainable agricultural systems but is currently a challenge in many areas. Using a combination of qualitative and quantitative participatory research methods, this study analyses rural youth’s realities, perspectives and aspirations in dryland Agricultural Livelihood Systems (ALSs) in the Midelt Province, Morocco, with a particular focus on gender. The data collected are an important first step in understanding the target group and working with youth to identify and develop appropriate programmatic interventions to improve their livelihoods and rural futures. Prior to expressing their aspirations for their rural life and career, the youth first raised the issue of unfulfilled primary needs: access to education, potable water, heath care, and lack of infrastructure in their villages. The issue of outmigration from rural areas is controversial and not so widespread. The youth’s dream village is envisioned as a rural place where people have a more comfortable life with their own families, farming better and more sustainably rather than seeking a job in urban areas. To support the youth’s aspirations and their willingness to stay in agriculture, there is a need for infrastructural and regulatory interventions and specific training in agricultural practices targeting and engaging youth.
Does Environmental Policy Reduce Enterprise Innovation?—Evidence from China
Authors: Feng Chen , Shi Beibei , Kang Rong
Published: May 2017

With the serious worldwide problem of carbon emissions, carbon emissions’ control and trade have become necessary policies adopted by the governments, after the establishment of the EU Emissions Trading System (EU ETS), China has also implemented a similar carbon emission trading pilot policy which plays a vital role in environmental regulation and influences enterprise behaviors, so this paper will put more focus on the impact of this policy on the enterprise innovation. In this paper, we construct a theoretical model and use the “Chinese Carbon Emissions Trading Pilot Policy” as a quasi-natural experiment to accurately identify the net causal effect of this environmental policy on enterprise innovation. Moreover, we use the synthetic control and difference-in-differences methods to eliminate the endogeneity to a large extent and conduct the robustness tests, difference-in-differences method, placebo test, and permutation test to respectively confirm these results. The results show that the implementation of carbon emissions trading policy will significantly reduce the enterprise innovation in general, this conclusion is contrary to the EU ETS’s effect, which is confirmed by plenty of previous empirical study. However, this policy has different effects across enterprises of different industries and different types of innovation. It can promote enterprise innovation of environmental industry, but it will inhibit enterprise innovation of non-environmental industry; besides, it can promote the development of green technical innovation and inhibit non-green technical innovation, which is basically identical to the causal effect of EU ETS.
Study of the Simulated Expansion Boundary of Construction Land in Shanghai Based on a SLEUTH Model
Authors: Shi Yishao , Wu Jie , Shi Shouzheng
Published: May 2017

The SLEUTH model is one of the recent methodological advances, but its data sources are mainly interpreted from remote sensing images. It doesn’t cover the whole urban area and exhibits technical errors. Moreover, the relationships between land use and land cover change (LUCC) and sustainability have seldom been discussed. Aimed at addressing these shortcomings, we focus on a wider range and use different data sources to simulate and predict the boundary of urban construction land expansion, and analyze the relationship between the expansion style of urban land and the change of ecological service value. Based on Shanghai land use map data, which covers the whole city scope and eliminates the error in data processing, we use a SLEUTH model to simulate the urban growth mode in Shanghai in 2020 under two scenarios, i.e., the natural growth mode and ecological priority mode, which are different from the previous scenario modes such as unprotected, light protected, moderately protected, and heavily protected modes. The results are as follows: (1) The development of urban land in Shanghai is most reasonable under the ecological priority mode as its ecological service value is higher than that of the natural growth mode; (2) Construction land expansion in Shanghai is very slow under the ecological priority mode, wherein construction land growth mainly occurs in the northwest, southwest, and southeast of Shanghai, and the northwest part of Chongming Island; (3) The surrounding area of the central city is given priority over edge growth, where the growth range is small. The outer suburbs are given priority for widespread growth, which is synchronous with the slow growth of roads.
Data Compatibility to Enhance Sustainable Capabilities for Autonomous Analytics in IoT
Authors: Razzaq Malik Kaleem , Habib Masood , Khalid Shehzad , Ullah Farhan , Umar Muhammad , Sajjad Taimur , Ahmad Awais
Published: May 2017

The collection of raw data is based on sensors embedded in devices or the environment for real-time data extraction. Nowadays, the Internet of Things (IoT) environment is used to support autonomous data collection by reducing human involvement. It is hard to analyze such data, especially when working with the sensors in a real-time environment. On using data analytics in IoT with the help of RDF, many issues can be resolved. Resultant data will have a better chance of quality analytics by reforming data into the semantical annotation. Industrial correspondence through data will be improved by the induction of semantics at large due to efficient data capturing, whereas one popular medium of sensors’ data storage is Relational Database (RDB). This study provides a complete automated mechanism to transform from loosely structured data stored in RDB into RDF. These data are obtained from sensors in semantically annotated RDF stores. The given study comprises methodology for improving compatibility by introducing bidirectional transformation between classical DB and RDF data stores to enhance the sustainable capabilities of IoT systems for autonomous analytics. Two case studies, one on weather and another on heart-rate measurement collections through IoT sensors, are used to show the transformation process in operation.
Sea Reclamation Status of Countries around the South China Sea from 1975 to 2010
Authors: Zhang Junjue , Su Fenzhen , Ding Zhi
Published: May 2017

As a way of turning sea into land for living space for humans, the actions of sea reclamation bring about significant benefits. Nevertheless, it is also an under-recognized threat to the environment and the marine ecosystem. Based on images in two periods, sea reclamation information of countries around the South China Sea was extracted from 1975 to 2010. The spatial state and driven forces of sea reclamation are then discussed. Results show that the overall strength of sea reclamation in the South China Sea was great. New reclaimed land added up to 3264 km2. Sea reclamation for fish farming was the main reclamation type and widely distributed in the whole area, especially on the coast from the Pearl River Delta to the Red River Delta, and the coast of Ca Mau Peninsula. Sea reclamation in China and Vietnam was rather significant, which occupies 80.6% of the total reclamation area. Singapore had the highest level of sea reclamation. New reclaimed land for fish farming holds a key role in China, Vietnam, and Indonesia, while new reclaimed land for construction and docks dominated in Malaysia, Singapore, and Brunei. Areas and use-type compositions of new reclaimed land in countries varied greatly due to the differences of economic factors, policy inclination, and landscapes in the respective countries.
Simulating Block-Level Urban Expansion for National Wide Cities
Authors: Long Ying , Wu Kang
Published: May 2017

Large-scale models are generally associated with large spatial modelling units, for example, counties or super grids (several to dozens of km2). Few applied urban models can achieve a large spatial coverage with irregular spatial units due to data availability and computation load. The framework of automatic identification and characterization of blocks developed by Liu and Long (2016) makes such an ideal model possible by establishing the existing urban blocks using road networks and points of interest for very large areas (e.g., a country or a continent). In this study, we develop a mega-vector-blocks cellular automata model (MVB-CA) to simulate urban expansion at the block level for 654 Chinese cities. The existing urban blocks in 2012 were used for initiating the MVB-CA and are generated using multi-levelled road networks and ubiquitous points of interest. We then simulate block-based urban expansion of all the cities from 2012 to 2017. The national spatial development strategies of China are discussed extensively by academia and policy makers, while the baseline scenario and other simulated urban expansion scenarios have been tested and compared horizontally. As one of the first block-based urban expansion models at a national scale, its academic contributions, practical applications, and potential biases are also discussed in this paper. The developed MVB-CA using general approaches is also applicable for other counties.
Sustainability and Innovation in the Automotive Sector: A Structured Content Analysis
Authors: Vaz Caroline , Rauen Tania , Lezana Álvaro
Published: May 2017

This study aims to analyse the scientific literature on sustainability and innovation in the automotive sector in the last 13 years. The research is classified as descriptive and exploratory. The process presented 31 articles in line with the research topic in the Scopus database. The bibliometric analysis identified the most relevant articles, authors, keywords, countries, research centers and journals for the subject from 2004 to 2016 in the Industrial Engineering domain. We concluded, through the systemic analysis, that the automotive sector is well structured on the issue of sustainability and process innovation. Innovations in the sector are of the incremental process type, due to the lower risk, lower costs and less complexity. However, the literature also points out that radical innovations are needed in order to fit the prevailing environmental standards. The selected studies show that environmental practices employed in the automotive sector are: the minimization of greenhouse gas emissions, life-cycle assessment, cleaner production, reverse logistics and eco-innovation. Thus, it displays the need for empirical studies in automotive companies on the environmental practices employed and how these practices impact innovation.
Lateralization of the Avian Magnetic Compass: Analysis of Its Early Plasticity
Authors: Gehring Dennis , Güntürkün Onur , Wiltschko Wolfgang , Wiltschko Roswitha
Published: May 2017

In European Robins, Erithacus rubecula, the magnetic compass is lateralized in favor of the right eye/left hemisphere of the brain. This lateralization develops during the first winter and initially shows a great plasticity. During the first spring migration, it can be temporarily removed by covering the right eye. In the present paper, we used the migratory orientation of robins to analyze the circumstances under which the lateralization can be undone. Already a period of 1½ h being monocularly left-eyed before tests began proved sufficient to restore the ability to use the left eye for orientation, but this effect was rather short-lived, as lateralization recurred again within the next 1½ h. Interpretable magnetic information mediated by the left eye was necessary for removing the lateralization. In addition, monocularly, the left eye seeing robins could adjust to magnetic intensities outside the normal functional window, but this ability was not transferred to the “right-eye system”. Our results make it clear that asymmetry of magnetic compass perception is amenable to short-term changes, depending on lateralized stimulation. This could mean that the left hemispheric dominance for the analysis of magnetic compass information depends on lateralized interhemispheric interactions that in young birds can swiftly be altered by environmental effects.
Communities of Practice as Systems: The Case of TEALEAF
Authors: McCloughlin Thomas
Published: May 2017

This work provides an update to the Erasmus Plus TEALEAF project. The outcomes of a week-long EU-funded/Irish Government-recognized teacher course in July 2016 is described within the context of a qualitative small-scale study investigating teachers’ progress in the course. The explicit aim of the teacher course was to equip a diverse group of teachers with the initial tools to work to produce simple digital apps for learning about biodiversity in their respective domains. A community of practice seeks to establish a new concept of the pre-existing generalised collective conscience through triangulated conversation between the generalised and particularised collective and individual consciences; in particular, to revise the generalised collective conscience that teachers can program apps for learning about biodiversity. A number of features of teaching and learning were selected in general and their relationship to constructivism delineated. The teachers were prompted for their responses to each day of the course through a self-evaluation tool and the responses were ranked according to the rubric. The data was analyzed using multidimensional scaling—ASCAL procedure—in SPSS 23TM and within the repertory grid domain according to the RepSocio tool in Rep 5TM. The plots show a gradual development throughout the week in terms of specific features becoming ‘stronger’ or exerting more influence towards the middle of the course and fragmenting after that. Analyses were able to show which participants correlated most closely with the hypothetical ideal within the community of practice. Concerning this community of practice, the interrelationships within the community are determined using a social mapping exercise moving from the individual consciences to a particularised collective conscience.
Reliability and Degradation of Solar PV Modules—Case Study of 19-Year-Old Polycrystalline Modules in Ghana
Authors: Quansah David , Adaramola Muyiwa , Takyi Gabriel , Edwin Isaac
Published: May 2017

Fourteen (14) rack-mounted polycrystalline modules installed on the concrete roof of the solar energy applications laboratory at the Kwame Nkrumah University of Science and Technology (KNUST) in Ghana, a hot humid environment, were assessed after 19 years of continuous outdoor exposure. The physical state of the modules was documented using a visual inspection checklist. They were further assessed by current-voltage (I-V) characterization and thermal imaging. The modules were found to be in good physical state, except some bubbles on front side and minor discolouration/corrosion at edge of the cells. Compared with reference values, the performance decline of the modules observed over the exposure period was: nominal power (Pnom), 21% to 35%; short circuit current (Isc), 5.8% to 11.7%; open circuit voltage (Voc) 3.6% to 5.6% and 11.9% to 25.7% for fill factor (FF). It is hoped that this study will provide some helpful information to project developers, manufacturers and the research community on the long-term performance of PV modules in Ghana.
“Uremic Toxin” Section in the Journal Toxins: A Powerful Tool to Bundle and Advance Knowledge on Uremia
Authors: Vanholder Raymond
Published: May 2017

In the journal Toxins, a new section has been added to those already included, which will be specifically devoted to uremic toxins.[...]
Orientation of Head Lice on Human Hosts, and Consequences for Transmission of Pediculosis: The Head Lice Movement Studies
Authors: Heukelbach Jorg , Asenov André , Araújo Oliveira Fabíola , Araújo de Melo Iana , Dos Santos Queiroz Jéssica , Speare Rick , Ugbomoiko Uade
Published: May 2017

We performed head lice movement studies to elucidate factors influencing orientation and movement of head lice. Studies included observation of lice movements on hand and forearm at different positions of the upper limb; movements exposed to unshaved and shaved forearm; and movements with and without antennae. In 57 of 60 (95.0%) observations while holding the hand down, lice moved proximal, and 3 (5%) distal. While holding the hand up, 37/60 (61.7%) moved proximal, and 23 (38.3%) distal (p < 0.0001). On the unshaved limb, 29/30 (96.7%) moved proximal, with clockwise movements in 26/30 (86.7%). After shaving, 9/30 (30%) walked proximal and 18 (60%) distal, with 12/30 (40%) clockwise movements. After antennectomy, while holding the hand up, 16/25 (64%) lice did not move, 1 (4%) walked proximal, and 8 (32%) distal. While handing the hand down, 17/25 (68%) did not move, 5 (20%) walked proximal, and 3 (12%) distal. Transmission of head lice may not only occur by head-to-head contact, but also via head-to-body contact, with movement to the head against gravitational pull. Surface factors of hand and forearm may be important in orientation for lice, in addition to gravity. Movement of lice against gravity is not governed by organs in the antennae.
Cost and Relative Value of Road Kill Surveys for Enhanced Rabies Surveillance in Raccoon Rabies Management
Authors: Slate Dennis , Kirby Jordona , Morgan Daniel , Algeo Timothy , Trimarchi Charles , Nelson Kathleen , Rudd Robert , Randall Adam , Carrara Mark , Chipman Richard
Published: May 2017

Oral rabies vaccination (ORV) requires knowledge of the spatial-temporal distribution of rabies virus variants targeted for control. Rabies-exposure based public health surveillance alone may not provide a sound basis for ORV decisions. The value and cost of road kill surveys was evaluated for the late spring–early fall 2005–2007 as a part of enhanced rabies surveillance in northern New York, where raccoon rabies is enzootic and ORV has occurred since the late 1990s. Structured surveys were conducted to collect raccoons and other meso-carnivores for rabies testing at the New York State Rabies Laboratory. Of the 209 meso-carnivore heads collected and submitted for testing, 175 were testable by direct fluorescent antibody; none was rabid. Rabies was also not reported through public health surveillance in survey zones during 2005–2007. Overall, survey costs were $37,118 (2016 USD). Salaries and benefits accounted for 61% of costs, followed by fuel (22%), vehicle depreciation (14%), and sample shipping (3%). Mean daily distance driven was 303 ± 37 km and 381 ± 28 km for total road kills and raccoons, respectively. Costs/road kill collected and submitted was $176/all species and $224/raccoon. This study provides costs for planning road kill surveys and underscores the need to continually improve enhanced rabies surveillance approaches to support ORV decision making.
TMPRSS2 and MSPL Facilitate Trypsin-Independent Porcine Epidemic Diarrhea Virus Replication in Vero Cells
Authors: Shi Wen , Fan Wenlu , Bai Jing , Tang Yandong , Wang Li , Jiang Yanping , Tang Lijie , Liu Min , Cui Wen , Xu Yigang , Li Yijing
Published: May 2017

Type II transmembrane serine proteases (TTSPs) facilitate the spread and replication of viruses such as influenza and human coronaviruses, although it remains unclear whether TTSPs play a role in the progression of animal coronavirus infections, such as that by porcine epidemic diarrhea virus (PEDV). In this study, TTSPs including TMPRSS2, HAT, DESC1, and MSPL were tested for their ability to facilitate PEDV replication in Vero cells. Our results showed that TMPRSS2 and MSPL played significant roles in the stages of cell–cell fusion and virus–cell fusion, whereas HAT and DESC1 exhibited weaker effects. This activation may be involved in the interaction between TTSPs and the PEDV S protein, as the S protein extensively co-localized with TMPRSS2 and MSPL and could be cleaved by co-expression with TMPRSS2 or MSPL. Moreover, the use of Vero cells expressing TMPRSS2 and MSPL facilitated PEDV replication in the absence of exogenous trypsin. In sum, we identified two host proteases, TMPRSS2 and MSPL, which may provide insights and a novel method for enhancing viral titers, expanding virus production, and improving the adaptability of PEDV isolates in vitro.
Variation in the Genetic Repertoire of Viruses Infecting Micromonas pusilla Reflects Horizontal Gene Transfer and Links to Their Environmental Distribution
Authors: Finke Jan , Winget Danielle , Chan Amy , Suttle Curtis
Published: May 2017

Prasinophytes, a group of eukaryotic phytoplankton, has a global distribution and is infected by large double-stranded DNA viruses (prasinoviruses) in the family Phycodnaviridae. This study examines the genetic repertoire, phylogeny, and environmental distribution of phycodnaviruses infecting Micromonas pusilla, other prasinophytes and chlorophytes. Based on comparisons among the genomes of viruses infecting M. pusilla and other phycodnaviruses, as well as the genome from a host isolate of M. pusilla, viruses infecting M. pusilla (MpVs) share a limited set of core genes, but vary strongly in their flexible pan-genome that includes numerous metabolic genes, such as those associated with amino acid synthesis and sugar manipulation. Surprisingly, few of these presumably host-derived genes are shared with M. pusilla, but rather have their closest non-viral homologue in bacteria and other eukaryotes, indicating horizontal gene transfer. A comparative analysis of full-length DNA polymerase (DNApol) genes from prasinoviruses with their overall gene content, demonstrated that the phylogeny of DNApol gene fragments reflects the gene content of the viruses; hence, environmental DNApol gene sequences from prasinoviruses can be used to infer their overall genetic repertoire. Thus, the distribution of virus ecotypes across environmental samples based on DNApol sequences implies substantial underlying differences in gene content that reflect local environmental conditions. Moreover, the high diversity observed in the genetic repertoire of prasinoviruses has been driven by horizontal gene transfer throughout their evolutionary history, resulting in a broad suite of functional capabilities and a high diversity of prasinovirus ecotypes.
Manipulation of Viral MicroRNAs as a Potential Antiviral Strategy for the Treatment of Cytomegalovirus Infection
Authors: Deng Jiang , Xiao Jun , Ma Ping , Gao Bo , Gong Feng , Lv Liping , Zhang Yanyu , Xu Jinbo
Published: May 2017

Cytomegalovirus (CMV) infection leads to notable morbidity and mortality in immunosuppressed patients. Current antiviral drugs are effective but seriously limited in their long-term use due to their relatively high toxicity. In the present study, we characterized the expression of murine CMV microRNAs (MCMV miRNAs) both in vitro and in vivo. Although 29 miRNAs were detectable during in vitro infection, only 11 miRNAs (classified as Group 1) were detectable during in vivo infection, and as many as 18 viral miRNAs (classified as Group 2) were less detectable (<50% of animals) in both the liver and lungs. In addition, viral miRNA profiles in the blood revealed unstable and reduced expression. We next explored the in vitro effects of viral miRNAs on MCMV replication. The inhibition of Group 1 viral miRNAs had little effect on virus production, but transfected cells overexpressing miR-m01-3-5p, miR-M23-1-5p, miR-M55-1, and miR-m107-1-5p in Group 2 showed statistically lower viral loads than those transfected with control miRNA (29%, 29%, 39%, and 43%, respectively, versus control). Finally, we performed hydrodynamic injection of viral miRNA agomirs and observed lower levels of MCMV recurrence in the livers of animals overexpressing the miR-m01-3-5p or mcmv-miR-M23-1-5p agomirs compared with those of animals transfected with control agomir, confirming the antiviral effects of viral miRNA manipulation in vivo. Therefore, the manipulation of viral miRNA expression shows great therapeutic potential and represents a novel antiviral strategy for the miRNA-based treatment of cytomegalovirus infection.
ATP-Driven Contraction of Phage T3 Capsids with DNA Incompletely Packaged In Vivo
Authors: Serwer Philip , Wright Elena T.
Published: May 2017

Adenosine triphosphate (ATP) cleavage powers packaging of a double-stranded DNA (dsDNA) molecule in a pre-assembled capsid of phages that include T3. Several observations constitute a challenge to the conventional view that the shell of the capsid is energetically inert during packaging. Here, we test this challenge by analyzing the in vitro effects of ATP on the shells of capsids generated by DNA packaging in vivo. These capsids retain incompletely packaged DNA (ipDNA) and are called ipDNA-capsids; the ipDNA-capsids are assumed to be products of premature genome maturation-cleavage. They were isolated via preparative Nycodenz buoyant density centrifugation. For some ipDNA-capsids, Nycodenz impermeability increases hydration and generates density so low that shell hyper-expansion must exist to accommodate associated water. Electron microscopy (EM) confirmed hyper-expansion and low permeability and revealed that 3.0 mM magnesium ATP (physiological concentration) causes contraction of hyper-expanded, lowpermeability ipDNA-capsids to less than mature size; 5.0 mM magnesium ATP (border of supraphysiological concentration) or more disrupts them. Additionally, excess sodium ADP reverses 3.0 mM magnesium ATP-induced contraction and re-generates hyper-expansion. The Nycodenz impermeability implies assembly perfection that suggests selection for function in DNA packaging. These findings support the above challenge and can be explained via the assumption that T3 DNA packaging includes a back-up cycle of ATP-driven capsid contraction and hyper-expansion.
Evaluation and Comparison of the Pathogenicity and Host Immune Responses Induced by a G2b Taiwan Porcine Epidemic Diarrhea Virus (Strain Pintung 52) and Its Highly Cell-Culture Passaged Strain in Conventional 5-Week-Old Pigs
Authors: Chang Yen-Chen , Kao Chi-Fei , Chang Chia-Yu , Jeng Chian-Ren , Tsai Pei-Shiue , Pang Victor , Chiou Hue-Ying , Peng Ju-Yi , Cheng Ivan-Chen , Chang Hui-Wen
Published: May 2017

A genogroup 2b (G2b) porcine epidemic diarrhea virus (PEDV) Taiwan Pintung 52 (PEDVPT) strain was isolated in 2014. The pathogenicity and host antibody responses elicited by low-passage (passage 5; PEDVPT-P5) and high-passage (passage 96; PEDVPT-P96) PEDVPT strains were compared in post-weaning PEDV-seronegative pigs by oral inoculation. PEDVPT-P5-inoculation induced typical diarrhea during 1–9 days post inoculation with fecal viral shedding persisting for 26 days. Compared to PEDVPT-P5, PEDVPT-P96 inoculation induced none-to-mild diarrhea and lower, delayed fecal viral shedding. Although PEDVPT-P96 elicited slightly lower neutralizing antibodies and PEDV-specific immunoglobulin G (IgG) and immunoglobulin A (IgA) titers, a reduction in pathogenicity and viral shedding of the subsequent challenge with PEDVPT-P5 were noted in both PEDVPT-P5- and PEDVPT-P96-inoculated pigs. Alignment and comparison of full-length sequences of PEDVPT-P5 and PEDVPT-P96 revealed 23 nucleotide changes and resultant 19 amino acid substitutions in non-structure proteins 2, 3, 4, 9, 14, 15, spike, open reading frame 3 (ORF3), and membrane proteins with no detectable deletion or insertion. The present study confirmed the pathogenicity of the PEDVPT isolate in conventional post-weaning pigs. Moreover, data regarding viral attenuation and potency of induced antibodies against PEDVPT-P5 identified PEDVPT-P96 as a potential live-attenuated vaccine candidate.
Distinct Contributions of Autophagy Receptors in Measles Virus Replication
Authors: Petkova Denitsa , Verlhac Pauline , Rozières Aurore , Baguet Joël , Claviere Mathieu , Kretz-Remy Carole , Mahieux Renaud , Viret Christophe , Faure Mathias
Published: May 2017

Autophagy is a potent cell autonomous defense mechanism that engages the lysosomal pathway to fight intracellular pathogens. Several autophagy receptors can recognize invading pathogens in order to target them towards autophagy for their degradation after the fusion of pathogen-containing autophagosomes with lysosomes. However, numerous intracellular pathogens can avoid or exploit autophagy, among which is measles virus (MeV). This virus induces a complete autophagy flux, which is required to improve viral replication. We therefore asked how measles virus interferes with autophagy receptors during the course of infection. We report that in addition to NDP52/CALCOCO2 and OPTINEURIN/OPTN, another autophagy receptor, namely T6BP/TAXIBP1, also regulates the maturation of autophagosomes by promoting their fusion with lysosomes, independently of any infection. Surprisingly, only two of these receptors, NDP52 and T6BP, impacted measles virus replication, although independently, and possibly through physical interaction with MeV proteins. Thus, our results suggest that a restricted set of autophagosomes is selectively exploited by measles virus to replicate in the course of infection.
A Japanese Encephalitis Virus Vaccine Inducing Antibodies Strongly Enhancing In Vitro Infection Is Protective in Pigs
Authors: García-Nicolás Obdulio , Ricklin Meret , Liniger Matthias , Vielle Nathalie , Python Sylvie , Souque Philippe , Charneau Pierre , Summerfield Artur
Published: May 2017

The Japanese encephalitis virus (JEV) is responsible for zoonotic severe viral encephalitis transmitted by Culex mosquitoes. Although birds are reservoirs, pigs play a role as amplifying hosts, and are affected in particular through reproductive failure. Here, we show that a lentiviral JEV vector, expressing JEV prM and E proteins (TRIP/JEV.prME), but not JEV infection induces strong antibody-dependent enhancement (ADE) activities for infection of macrophages. Such antibodies strongly promoted infection via Fc receptors. ADE was found at both neutralizing and non-neutralizing serum dilutions. Nevertheless, in vivo JEV challenge of pigs demonstrated comparable protection induced by the TRIP/JEV.prME vaccine or heterologous JEV infection. Thus, either ADE antibodies cause no harm in the presence of neutralizing antibodies or may even have protective effects in vivo in pigs. Additionally, we found that both pre-infected and vaccinated pigs were not fully protected as low levels of viral RNA were found in lymphoid and nervous system tissue in some animals. Strikingly, the virus from the pre-infection persisted in the tonsils throughout the experiment. Finally, despite the vaccination challenge, viral RNA was detected in the oronasal swabs in all vaccinated pigs. These latter data are relevant when JEV vaccination is employed in pigs.
Unexpected Infection Spikes in a Model of Respiratory Syncytial Virus Vaccination
Authors: Smith Robert , Hogan Alexandra , Mercer Geoffry
Published: May 2017

Respiratory Syncytial Virus (RSV) is an acute respiratory infection that infects millions of children and infants worldwide. Recent research has shown promise for the development of a vaccine, with a range of vaccine types now in clinical trials or preclinical development. We extend an existing mathematical model with seasonal transmission to include vaccination. We model vaccination both as a continuous process, applying the vaccine during pregnancy, and as a discrete one, using impulsive differential equations, applying pulse vaccination. We develop conditions for the stability of the disease-free equilibrium and show that this equilibrium can be destabilised under certain extreme conditions, even with 100% coverage using an (unrealistic) vaccine. Using impulsive differential equations and introducing a new quantity, the impulsive reproduction number, we showed that eradication could be acheived with 75% coverage, while 50% coverage resulted in low-level oscillations. A vaccine that targets RSV infection has the potential to significantly reduce the overall prevalence of the disease, but appropriate coverage is critical.
The Use of Molluscan Fauna as Model Taxon for the Ecological Classification of River Estuaries
Authors: Itsukushima Rei , Morita Kai , Shimatani Yukihiro
Published: May 2017

River estuaries are important aquatic environments characterized by large environmental gradients in their water quality, riverbed material, and microtopography in the longitudinal and transverse directions. The geography or habitats in river estuaries differ depending on the energy from the tide, waves, and river; therefore, the biota inhabiting river estuaries vary depending on the river estuary type. In view of this, for effective conservation in river estuaries, there is a need for information about potential habitats and biota based on objective data about the river estuary type. The objective of this study thus was to classify river estuaries by their molluscan fauna and physical indicators to reveal the relationship between molluscan fauna and the physical environment. The classification results using physical indicators indicated three types of river estuaries (wave energy-dominated group, tide energy-dominated group, and low tide and wave energy group). This classification result was similar to the classification of molluscan fauna. Therefore, it was suggested that molluscan fauna is extremely useful as a variable representing the river estuary environment. From the comparison between molluscan fauna and the physical environment, some rivers were not classified into the same group in the classification of molluscan fauna, despite them having similar physical environments. Some of these rivers with a molluscan fauna that diverged from expectations had undergone channel modification, which is expected to have caused a shift in this fauna group. These results suggest that this approach could be used to identify rivers that have been degraded by human activities.
Rooftop Rainwater Harvesting for Mombasa: Scenario Development with Image Classification and Water Resources Simulation
Authors: Ojwang Robert O. , Dietrich Jörg , Anebagilu Prajna Kasargodu , Beyer Matthias , Rottensteiner Franz
Published: May 2017

Mombasa faces severe water scarcity problems. The existing supply is unable to satisfy the demand. This article demonstrates the combination of satellite image analysis and modelling as tools for the development of an urban rainwater harvesting policy. For developing a sustainable remedy policy, rooftop rainwater harvesting (RRWH) strategies were implemented into the water supply and demand model WEAP (Water Evaluation and Planning System). Roof areas were detected using supervised image classification. Future population growth, improved living standards, and climate change predictions until 2035 were combined with four management strategies. Image classification techniques were able to detect roof areas with acceptable accuracy. The simulated annual yield of RRWH ranged from 2.3 to 23 million cubic meters (MCM) depending on the extent of the roof area. Apart from potential RRWH, additional sources of water are required for full demand coverage.