bluuebunny/crossref_metadata_2025 huggingface dataset:
The role of Annexin A3 in coronary arterial lesions in children with Kawasaki disease
Authors: Li Mengling , Liu Dong , Jing Fengchuan , Liu Ruixi , Yi Qijian
Published: February 2023

Kawasaki disease (KD) is an acute, self-limited vasculitis, and the etiology is still unclear. Coronary arterial lesions (CALs) are a major complication of KD. Excessive inflammation and immunologic abnormities are involved in the pathogenesis of KD and CALs. Annexin A3 (ANXA3) plays crucial roles in cell migration and differentiation, inflammation, cardiovascular and membrane metabolic diseases. The purpose of this study was to investigate the effect of ANXA3 on the pathogenesis of KD and CALs. There were 109 children with KD in the KD group [which was divided into two groups: 67 patients with CALs in the KD-CAL group, and 42 patients with noncoronary arterial lesions (NCALs) in the KD-NCAL group] and 58 healthy children in the control (HC) group. Clinical and laboratory data were retrospectively collected from all patients with KD. The serum concentration of ANXA3 was measured by enzyme-linked immunosorbent assays (ELISAs). Serum ANXA3 levels were higher in the KD group than in the HC group (P < 0.05). There was a higher concentration of serum ANXA3 in the KD-CAL group than in the KD-NCAL group (P < 0.05). Neutrophil cell counts and serum ANXA3 levels were higher in the KD group than in the HC group (P < 0.05) and quickly decreased when the patients were treated with IVIG after 7 days of illness. Platelet (PLT) counts and ANXA3 levels concurrently exhibited significant increases 7 days after onset. Furthermore, ANXA3 levels were positively correlated with lymphocyte and PLT counts in the KD and KD-CAL groups. ANXA3 may be involved in the pathogenesis of KD and CALs.
A comprehensive overview of clinical research on dexmedetomidine in the past 2 decades: A bibliometric analysis
Authors: Kong Hao , Li Mo , Deng Chun-Mei , Wu Yu-Jia , He Shu-Ting , Mu Dong-Liang
Published: February 2023

Introduction: Dexmedetomidine is a potent, highly selective α-2 adrenoceptor agonist with sedative, analgesic, anxiolytic, and opioid-sparing properties. A large number of dexmedetomidine-related publications have sprung out in the last 2 decades. However, no bibliometric analysis for clinical research on dexmedetomidine has been published to analyze hot spots, trends, and frontiers in this field.

Methods: The clinical articles and reviews related to dexmedetomidine, published from 2002 to 2021 in the Web of Science Core Collection, were retrieved on 19 May 2022, using relevant search terms. VOSviewer and CiteSpace were used to conduct this bibliometric study.

Results: The results showed that a total of 2,299 publications were retrieved from 656 academic journals with 48,549 co-cited references by 2,335 institutions from 65 countries/regions. The United States had the most publications among all the countries (n = 870, 37.8%) and the Harvard University contributed the most among all institutions (n = 57, 2.48%). The most productive academic journal on dexmedetomidine was Pediatric Anesthesia and the first co-cited journal was Anesthesiology. Mika Scheinin is the most productive author and Pratik P Pandharipande is the most co-cited author. Co-cited reference analysis and keyword analysis illustrated hot spots in the dexmedetomidine field including pharmacokinetics and pharmacodynamics, intensive care unit sedation and outcome, pain management and nerve block, and premedication and use in children. The effect of dexmedetomidine sedation on the outcomes of critically ill patients, the analgesic effect of dexmedetomidine, and its organ protective property are the frontiers in future research.

Conclusion: This bibliometric analysis provided us with concise information about the development trend and provided an important reference for researchers to guide future research.
Systematic investigation of the underlying mechanisms of GLP-1 receptor agonists to prevent myocardial infarction in patients with type 2 diabetes mellitus using network pharmacology
Authors: Deng Guorong , Ren Jiajia , Li Ruohan , Li Minjie , Jin Xuting , Li Jiamei , Liu Jueheng , Gao Ya , Zhang Jingjing , Wang Xiaochuang , Wang Gang
Published: February 2023

Background: Several clinical trials have demonstrated that glucagon-like peptide-1 (GLP-1) receptor agonists (GLP-1RAs) reduce the incidence of non-fatal myocardial infarction (MI) in patients with type 2 diabetes mellitus (T2DM). However, the underlying mechanism remains unclear. In this study, we applied a network pharmacology method to investigate the mechanisms by which GLP-1RAs reduce MI occurrence in patients with T2DM.

Methods: Targets of three GLP-1RAs (liraglutide, semaglutide, and albiglutide), T2DM, and MI were retrieved from online databases. The intersection process and associated targets retrieval were employed to obtain the related targets of GLP-1RAs against T2DM and MI. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genes (KEGG) enrichment analyses were performed. The STRING database was used to obtain the protein-protein interaction (PPI) network, and Cytoscape was used to identify core targets, transcription factors, and modules.

Results: A total of 198 targets were retrieved for the three drugs and 511 targets for T2DM with MI. Finally, 51 related targets, including 31 intersection targets and 20 associated targets, were predicted to interfere with the progression of T2DM and MI on using GLP-1RAs. The STRING database was used to establish a PPI network comprising 46 nodes and 175 edges. The PPI network was analyzed using Cytoscape, and seven core targets were screened: AGT, TGFB1, STAT3, TIMP1, MMP9, MMP1, and MMP2. The transcription factor MAFB regulates all seven core targets. The cluster analysis generated three modules. The GO analysis for 51 targets indicated that the terms were mainly enriched in the extracellular matrix, angiotensin, platelets, and endopeptidase. The results of KEGG analysis revealed that the 51 targets primarily participated in the renin-angiotensin system, complement and coagulation cascades, hypertrophic cardiomyopathy, and AGE-RAGE signaling pathway in diabetic complications.

Conclusion: GLP-1RAs exert multi-dimensional effects on reducing the occurrence of MI in T2DM patients by interfering with targets, biological processes, and cellular signaling pathways related to atheromatous plaque, myocardial remodeling, and thrombosis.
Toripalimab plus chemotherapy vs. chemotherapy in patients with advanced non-small-cell lung cancer: A cost-effectiveness analysis
Authors: Huo Gengwei , Liu Wenjie , Kang Shuo , Chen Peng
Published: February 2023

Background: The potency and safety of toripalimab combination with chemotherapy (TC) as the first-line therapy for advanced non-small cell lung cancer (NSCLC) have been demonstrated in the CHOICE-01 study. Our research explored whether TC was cost-effective compared to chemotherapy alone from the Chinese payer perspective.

Materials and methods: Clinical parameters were obtained from a randomized, multicenter, registrational, placebo-controlled, double-blind, phase III trial. Standard fee databases and previously published literature were used to determine costs and utilities. A Markov model with three mutually exclusive health statuses (progression-free survival (PFS), disease progression, and death) was used to predict the disease course. The costs and utilities were discounted at 5% per annum. The main endpoints of the model included cost, quality-adjusted life years (QALYs), and incremental cost-effectiveness ratio (ICER). Univariate and probabilistic sensitivity analyses were performed to investigate the uncertainty. Subgroup analyses were performed to verify the cost-effectiveness of TC in patients with squamous and non-squamous cancer.

Results: TC combination therapy yielded an incremental 0.54 QALYs with an incremental cost of $11,777, compared to chemotherapy, giving rise to ICERs of $21,811.76/QALY. Probabilistic sensitivity analysis revealed that TC was not favorable at 1 time GDP per capita. With a prespecified willingness-to-pay threshold (WTP) of three times the GDP per capita, combined treatment had a 100% probability of being cost-effective and had substantial cost-effectiveness in advanced NSCLC. Probabilistic sensitivity analyses showed that TC was more likely to be accepted with a WTP threshold higher than $22,195 in NSCLC. Univariate sensitivity analysis showed that the utility of PFS state, crossover proportions of the chemotherapy arm, cost per cycle of pemetrexed treatment, and discount rate were the dominant influencing factors. Subgroup analyses found that in patients with squamous NSCLC, the ICER was $14,966.09/QALY. In the non-squamous NSCLC, ICER raised to $23,836.27/QALY. ICERs were sensitive to the variance of the PFS state utility. TC was more likely to be accepted when WTP increases exceeded $14,908 in the squamous NSCLC subgroup and $23,409 in the non-squamous NSCLC subgroup.

Conclusion: From the perspective of the Chinese healthcare system, TC may be cost-effective in individuals with previously untreated advanced NSCLC at the prespecified WTP threshold compared to chemotherapy, and more significant in individuals with squamous NSCLC, which will provide evidence for clinicians to make the best decisions in general clinical practice.
Atrial fibrillation classification based on the 2D representation of minimal subset ECG and a non-deep neural network
Authors: Zhang Hua , Liu Chengyu , Tang Fangfang , Li Mingyan , Zhang Dongxia , Xia Ling , Crozier Stuart , Gan Hongping , Zhao Nan , Xu Wenlong , Liu Feng
Published: February 2023

Atrial fibrillation (AF) is the most common cardiac arrhythmia, and its early detection is critical for preventing complications and optimizing treatment. In this study, a novel AF prediction method is proposed, which is based on investigating a subset of the 12-lead ECG data using a recurrent plot and ParNet-adv model. The minimal subset of ECG leads (II &V1) is determined via a forward stepwise selection procedure, and the selected 1D ECG data is transformed into 2D recurrence plot (RP) images as an input to train a shallow ParNet-adv Network for AF prediction. In this study, the proposed method achieved F1 score of 0.9763, Precision of 0.9654, Recall of 0.9875, Specificity of 0.9646, and Accuracy of 0.9760, which significantly outperformed solutions based on single leads and complete 12 leads. When studying several ECG datasets, including the CPSC and Georgia ECG databases of the PhysioNet/Computing in Cardiology Challenge 2020, the new method achieved F1 score of 0.9693 and 0.8660, respectively. The results suggested a good generalization of the proposed method. Compared with several state-of-art frameworks, the proposed model with a shallow network of only 12 depths and asymmetric convolutions achieved the highest average F1 score. Extensive experimental studies proved that the proposed method has a high potential for AF prediction in clinical and particularly wearable applications.
Six weeks of N-acetylcysteine antioxidant in drinking water decreases pathological fiber branching in MDX mouse dystrophic fast-twitch skeletal muscle
Authors: Redwan Asma , Kiriaev Leonit , Kueh Sindy , Morley John W. , Houweling Peter , Perry Ben D. , Head Stewart I.
Published: February 2023

Introduction: It has been proposed that an increased susceptivity to oxidative stress caused by the absence of the protein dystrophin from the inner surface of the sarcolemma is a trigger of skeletal muscle necrosis in the destructive dystrophin deficient muscular dystrophies. Here we use the mdx mouse model of human Duchenne Muscular Dystrophy to test the hypothesis that adding the antioxidant NAC at 2% to drinking water for six weeks will treat the inflammatory phase of the dystrophic process and reduce pathological muscle fiber branching and splitting resulting in a reduction of mass in mdx fast-twitch EDL muscles.

Methods: Animal weight and water intake was recorded during the six weeks when 2% NAC was added to the drinking water. Post NAC treatment animals were euthanised and the EDL muscles dissected out and placed in an organ bath where the muscle was attached to a force transducer to measure contractile properties and susceptibility to force loss from eccentric contractions. After the contractile measurements had been made the EDL muscle was blotted and weighed. In order to assess the degree of pathological fiber branching mdx EDL muscles were treated with collagenase to release single fibers. For counting and morphological analysis single EDL mdx skeletal muscle fibers were viewed under high magnification on an inverted microscope.

Results: During the six-week treatment phase NAC reduced body weight gain in three- to nine-week-old mdx and littermate control mice without effecting fluid intake. NAC treatment also significantly reduced the mdx EDL muscle mass and abnormal fiber branching and splitting.

Discussion: We propose chronic NAC treatment reduces the inflammatory response and degenerative cycles in the mdx dystrophic EDL muscles resulting in a reduction in the number of complexed branched fibers reported to be responsible for the dystrophic EDL muscle hypertrophy.
Identification of candidate genes for LepR1 resistance against Leptosphaeria maculans in Brassica napus
Authors: Cantila Aldrin Y. , Thomas William J.W. , Saad Nur Shuhadah Mohd , Severn-Ellis Anita A. , Anderson Robyn , Bayer Philipp E. , Edwards David , Van de Wouw Angela P. , Batley Jacqueline
Published: February 2023

Utilising resistance (R) genes, such as LepR1, against Leptosphaeria maculans, the causal agent of blackleg in canola (Brassica napus), could help manage the disease in the field and increase crop yield. Here we present a genome wide association study (GWAS) in B. napus to identify LepR1 candidate genes. Disease phenotyping of 104 B. napus genotypes revealed 30 resistant and 74 susceptible lines. Whole genome re-sequencing of these cultivars yielded over 3 million high quality single nucleotide polymorphisms (SNPs). GWAS in mixed linear model (MLM) revealed a total of 2,166 significant SNPs associated with LepR1 resistance. Of these SNPs, 2108 (97%) were found on chromosome A02 of B. napus cv. Darmor bzh v9 with a delineated LepR1_mlm1 QTL at 15.11-26.08 Mb. In LepR1_mlm1, there are 30 resistance gene analogs (RGAs) (13 nucleotide-binding site-leucine rich repeats (NLRs), 12 receptor-like kinases (RLKs), and 5 transmembrane-coiled-coil (TM-CCs)). Sequence analysis of alleles in resistant and susceptible lines was undertaken to identify candidate genes. This research provides insights into blackleg resistance in B. napus and assists identification of the functional LepR1 blackleg resistance gene.
Effects of different plantation years on grassland plant community in Maxian Mountain area of the Loess Plateau
Authors: Mao Liang , Li Jie , Ma Xiao-Long , Quandahor Peter , Gou Yu-Ping
Published: February 2023

Plant communities in the Loess Plateau’s artificial afforestation forests play an important role in fragile ecosystem restoration. Therefore, the composition, coverage, biomass, diversity, and similarity of grassland plant communities in different years of artificial afforestation in cultivated land were investigated. The effects of years of artificial afforestation on grassland plant community succession in the Loess Plateau were also investigated. The results showed that as the number of years of artificial afforestation increased, grassland plant communities grew from scratch, constantly optimizing community components, improving community coverage, and increasing aboveground biomass. The community diversity index and similarity coefficient gradually approached those of a 10-year abandoned community that had recovered naturally. After 6 years of artificial afforestation, the dominant species of the grassland plant community changed from Agropyron cristatum to Kobresia myosuroides, and the main associated species changed from Compositae and Gramineae to Compositae, Gramineae, Rosaceae, and Leguminosae. The α-diversity index accelerated restoration, the richness index and diversity index increased, and the dominant index decreased. The evenness index had no significant difference from CK. The β-diversity index decreased as the number of years of afforestation increased. The similarity coefficient between CK and grassland plant communities in various lands changed from medium dissimilarity to medium similarity at 6 years of afforestation. According to the analysis of various indicators of the grassland plant community, the grassland plant community had a positive succession within 10 years of artificial afforestation on the cultivated land of the Loess Plateau, and the threshold of the years from slow to fast was 6 years.
Spatial and temporal variations of net ecosystem productivity in Xinjiang Autonomous Region, China based on remote sensing
Authors: Lu Xiangjun , Chen Yang , Sun Yuyin , Xu Yongming , Xin Yan , Mo Yaping
Published: February 2023

Net ecosystem productivity (NEP), which plays a key role in the carbon cycle, is an important indicator of the ecosystem's carbon budget. In this paper, the spatial and temporal variations of NEP over Xinjiang Autonomous Region, China from 2001 to 2020 were studied based on remote sensing and climate re-analysis data. The modified Carnegie Ames Stanford Approach (CASA) model was employed to estimate net primary productivity (NPP), and the soil heterotrophic respiration model was used to calculate soil heterotrophic respiration. Then NEP was obtained by calculating the difference between NPP and heterotrophic respiration. The annual mean NEP of the study area was high in the east and low in the west, high in the north and low in the south. The 20-year mean vegetation NEP of the study area is 128.54 gC·m-2, indicating that the study area is a carbon sink on the whole. From 2001 to 2020, the annual mean vegetation NEP ranged between 93.12 and 158.05 gC·m-2, and exhibited an increasing trend in general. 71.46% of the vegetation area showed increasing trends of NEP. NEP exhibited a positive relationship with precipitation and a negative relationship with air temperature, and the correlation with air temperature was more significant. The work reveals the spatio-temporal dynamics of NEP in Xinjiang Autonomous Region and can provide a valuable reference for assessing regional carbon sequestration capacity.
Putting coastal communities at the center of a sustainable blue economy: A review of risks, opportunities, and strategies
Authors: Evans Louisa S. , Buchan Pamela M. , Fortnam Matt , Honig Maria , Heaps Louise
Published: February 2023

New approaches to ocean governance for coastal communities are needed. With few exceptions, the status quo does not meet the diverse development aspirations of coastal communities or ensure healthy oceans for current and future generations. The blue economy is expected to grow to USD2.5–3 trillion by 2030, and there is particular interest in its potential to alleviate poverty in Least Developed Countries and Small Island Developing States, and to support a blue recovery from the COVID-19 pandemic. This paper presents a selective, thematic review of the blue economy literature to examine: (i) the opportunities and risks for coastal communities, (ii) the barriers and enablers that shape community engagement, and (iii) the strategies employed by communities and supporting organizations, which can be strengthened to deliver a ‘sustainable' blue economy and improve social justice for coastal communities. Our review finds that under business-as-usual and blue growth, industrial fisheries, large-scale aquaculture, land reclamation, mining, and oil and gas raise red flags for communities and marine ecosystems. Whereas, if managed sustainably, small-scale fisheries, coastal aquaculture, seaweed farming and eco-tourism are the most likely to deliver benefits to communities. Yet, these are also the sectors most vulnerable to negative and cumulative impacts from other sectors. Based on our evaluation of enablers, barriers and strategies, the paper argues that putting coastal communities at the center of a clear vision for an inclusive Sustainable Blue Economy and co-developing a shared and accessible language for communities, practitioners and policy-makers is essential for a more equitable ocean economy, alongside mainstreaming social justice principles and integrated governance that can bridge different scales of action and opportunity.
How the home learning environment contributes to children’s social–emotional competence: A moderated mediation model
Authors: Li Shaomei , Tang Yu , Zheng Yuxin
Published: February 2023

Introduction

The home learning environment is the earliest contact learning environment in early childhood development, which plays an important role in the development of children’s social-emotional competence. However, previous studies have not clarified the precise mechanisms by which the home learning environment influences children’s social-emotional competence. Therefore, the purpose of the study is to explore the relationship between the home learning environment and its intrinsic structure (i.e. structural family characteristics, parental beliefs and interests, and the educational processes) and children’s social-emotional competence, and whether gender plays a moderating role in the relationship.

Method

The study randomly selected a sample of 443 children from 14 kindergartens in western China. The Home Learning Environment Questionnaire and the Chinese Inventory of Children’s Social-emotional competence scale were used to investigate the home learning environment and social-emotional competence of these children.

Results

(1) Structural family characteristics and parental beliefs and interests both had a significant positive predictive effect on children’s social-emotional competence. (2) The educational processes fully mediate between structural family characteristics, parental beliefs and interests, and children’s social-emotional competence. (3) Gender moderated the effect of the home learning environment on children’s social-emotional competence. Gender moderates not only the indirect effects between parental beliefs and interests and children’s social-emotional competence, but also the indirect effects between structural family characteristics and children’s social-emotional competence. At the same time, gender also moderated the direct effects between parental beliefs and interests and children’s social-emotional competence.

Discussion

The results emphasize the crucial role of the home learning environment in the development of children’s early social-emotional competence. Therefore, parents should pay attention to the home learning environment and improve their ability to create a home learning environment that promotes the positive development of children’s social-emotional competence.
Development and psychometric testing of a scale for assessing the psychological birth trauma
Authors: Zhang Ke , Wu Meiliyang , Zeng Tieying , Yuan Mengmei , Chen Ye , Yang Lingyan
Published: February 2023

Background

Psychological birth trauma is widespread in postpartum women, and its harms are serious to mothers’ health. Existing tools rely on posttraumatic stress disorder to evaluate, which cannot effectively evaluate its connotation. The aim of this study was to develop a new instrument for use to comprehensively assess the psychological birth trauma level of women after birth and test the scale’s psychometric properties.

Methods

The scale was developed and evaluated through item generation, expert consultation, pre-survey, and psychometric evaluation. A literature review, focus group, and individual deep semi-structured interviews were utilized to identify the scale items. The expert consultation evaluated the content validity. Psychometric testing was conducted in a convenience sample of 712 mothers within the first 72 h postpartum who were recruited from three hospitals in China.

Results

The total Cronbach alpha coefficient of the scale was 0.874. Exploratory factor analysis supported that the final scale consisted of four dimensions and fifteen items. The explanatory variance of the four factors was 66.724%. The four dimensions are named “being neglected,” “out of control,” “physiological emotional response,” and “cognitive behavioral response.” The results of the confirmatory factor analysis showed that the fit indices were all at acceptable and good levels.

Conclusion

The 15-item Birth Trauma Scale is a valid and reliable tool to evaluate the psychological trauma of mothers who experienced spontaneous childbirth. The scale is a maternal self-assessment scale that can help women understand their mental health. Healthcare providers can identify key populations and intervene with them.
Frequency of needle stick injuries among healthcare providers during large-scale SARS-CoV-2 vaccination brigades
Authors: Moran-González Jorge Daniel , Padilla-Orozco Magaly , Guzman-Lopez Abel , Ochoa-Bayona Hilda Cristina , Camacho-Ortiz Adrian
Published: February 2023

The frequency of needle stick-related accidents in large-scale vaccination brigades during the COVID-19 pandemic is unknown. We determined the incidence of needle stick injuries (NIs) from the SARS-CoV-2 vaccination brigades in the Monterrey metropolitan area. We calculated the rate of NI by 100,000 doses administered from a registry of over 4 million doses.
The evolution of HIV self-testing and the introduction of digital interventions to improve HIV self-testing
Authors: Fischer Alex Emilio , Abrahams Musaed , Shankland Luke , Lalla-Edward Samanta Tresha , Edward Vinodh A. , De Wit John
Published: February 2023

HIV self-testing (HIVST) complements traditional HIV testing programmes by removing barriers and increasing access to testing for key populations, and digital interventions have been developed for HIVST to improve the testing and linkage to care experience for users. The first HIVST kit was proposed in 1986, but it took 10 years for the home sample collection (HSC) HIVST to become available and another 16 years for rapid diagnostic test HIVST to be approved by the Federal Drug Administration. Since then, studies have shown high usability and performance of HIVST, which led the World Health Organization formally recommending HIVST in 2016, and currently almost 100 countries have incorporated HIVST into their national testing strategy. Despite the popularity, HIVST present challenges around pre-and post-test counselling, as well as the ability to report results and link users to care, and digital interventions for HIVST have been introduced to address these challenges. The first digital intervention for HIVST was introduced in 2014 and showed that digital interventions could be used to distribute HIVST kits, report results and link users to care. Since then, dozens of studies have been conducted, which have validated and expanded on these early findings, but many were pilot studies with small sample sizes and lacked the standardization of indicators required to aggregate data across platforms to prove impact at scale. For digital interventions for HIVST to be championed for scale-up, they must continue to show measurable impact at larger scales, while still maintaining and standardizing data security and integrity.
Archaeological soil from Roman occupational layers can be differentiated by microbial and chemical signatures
Authors: Taylor Gillian , Williams Rhys , Halldórsdóttir Hrafnhildur Helga , Carter Ashleigh , Birley Andrew , Meyer Alexander , Orr Caroline Hayley
Published: February 2023

Introduction

Soil at the Roman site of Vindolanda (Northumberland, UK) provides excellent preservation of wooden artefacts including Roman writing tablets.

Methods

In this study we examined chemical and microbial signature changes within varied occupation contexts of archaeological soil. Analysis included investigating elemental composition, sterol biomarkers, bacterial diversity and community structures from excavation trenches at Vindolanda using pXRF, GC-MS and 16S rRNA gene amplicon sequencing. Samples were taken from varying depths starting at topsoil and working down through layers of Roman occupation including one cavalry stable floor, two infantry barracks and a cook house, and layers which contained Roman writing tablets.

Results and Discussion

The chemical results indicate that areas where wooden artefacts were found had increased soil moisture which was also correlated with specific chemical conditions including shifts in iron, sulphur and phosphorous concentration. Steroid biomarkers indicate the presence of faecal matter in layers, supporting occupation descriptions. Overall microbial diversity did not change across the depth profile but was correlated with soil moisture. Anaerobic soils associated with more optimal preservation differed to other soils with increases in Firmicutes, Proteobacteria, Campilobacterota and Bacteroidota observed. Microbial community structure and putative function as revealed by PICRUSt2 is linked to occupation usage rather than depth of samples with laminated floor layers differing from turf structures. Understanding the complex processes within archaeological soil can help us to understand dynamics of decomposition and preservation. In addition, the apparent preservation of the environmental microbial community as well as the artefacts themselves allows us to understand the microbial environments of the past, how they relate to the present and what this means for our changing environments in the future.
Clinical diagnosis model of spinal meningiomas based on the surveillance, epidemiology, and end results database
Authors: Jiang Yong’An , Chen Peng , Liang JiaWei , Long XiaoYan , Cai JiaHong , Zhang Yi , Cheng ShiQi , Zhang Yan
Published: February 2023

Most spinal meningiomas (SM) are benign lesions of the thoracic spine and are usually treated surgically. This study aimed to explore treatment strategies and construct a nomogram for SM. Data on patients with SM from 2000 to 2019 were extracted from the Surveillance, Epidemiology, and End Results database. First, the distributional properties and characteristics of the patients were descriptively evaluated, and the patients were randomly divided into training and testing groups in a 6:4 ratio. Least absolute shrinkage and selection operator (LASSO) regression was used to screen the survival predictors. Kaplan–Meier curves explained survival probability by different variables. The nomogram was constructed based on the results of LASSO regression. The predictive power of the nomogram was identified using the concordance index, time-receiver operating characteristics, decision curve analysis, and calibration curves. We recruited 1,148 patients with SM. LASSO results for the training group showed that sex (coefficient, 0.004), age (coefficient, 0.034), surgery (coefficient, −0.474), tumor size (coefficient, 0.008), and marital status (coefficient, 0.335) were prognostic factors. The nomogram prognostic model showed good diagnostic ability in both the training and testing groups, with a C-index of 0.726, 95% (0.679, 0.773); 0.827, 95% (0.777, 0.877). The calibration and decision curves suggested that the prognostic model had better diagnostic performance and good clinical benefit. In the training and testing groups, the time-receiver operating characteristic curve showed that SM had moderate diagnostic ability at different times, and the survival rate of the high-risk group was significantly lower than that of the low-risk group (training group: p = 0.0071; testing group: p = 0.00013). Our nomogram prognostic model may have a crucial role in predicting the six-month, one-year, and two-year survival outcomes of patients with SM and may be useful for surgical clinicians to formulate treatment plans.
Is Following a Cancer-Protective Lifestyle Linked to Reduced Cancer Mortality Risk?
Authors: Suter Flurina , Karavasiloglou Nena , Braun Julia , Pestoni Giulia , Rohrmann Sabine
Published: February 2023

Objectives: This study investigates the association between a cancer protective lifestyle (defined based on the revised World Cancer Research Fund (WCRF) and the American Institute for Cancer Research (AICR) cancer prevention recommendations) and mortality in Switzerland.

Methods: Based on the cross-sectional, population-based National Nutrition Survey, menuCH (n = 2057), adherence to the WCRF/AICR recommendations was assessed via a score. Quasipoisson regression models were fitted to examine the association of adherence to the WCRF/AICR recommendations with mortality at the Swiss district-level. Spatial autocorrelation was tested with global Moran’s I. Integrated nested Laplace approximation models were fitted when significant spatial autocorrelation was detected.

Results: Participants with higher cancer prevention scores had a significant decrease in all-cause (relative risk 0.95; 95% confidence interval 0.92, 0.99), all-cancer (0.93; 0.89, 0.97), upper aero-digestive tract cancer (0.87; 0.78, 0.97), and prostate cancer (0.81; 0.68, 0.94) mortality, compared to those with lower scores.

Conclusion: The inverse association between adherence to the WCRF/AICR recommendations and mortality points out the potential of the lifestyle recommendations to decrease mortality and especially the burden of cancer in Switzerland.
An Intellectual Aerodynamic Design Method for Compressors Based on Deep Reinforcement Learning
Authors: Xu Xiaohan , Huang Xudong , Bi Dianfang , Zhou Ming
Published: February 2023

Aerodynamic compressor designs require considerable prior knowledge and a deep understanding of complex flow fields. With the development of computer science, artificial intelligence (AI) has been widely applied to compressors design. Among the various AI models, deep reinforcement learning (RL) methods have successfully addressed complex problems in different domains. This paper proposes a modified deep deterministic policy gradient algorithm for compressor design and trains several agents, improving the performance of a 3D transonic rotor for the first time. An error reduction process was applied to improve the capability of the surrogate models, and then RL environments were established based on the surrogate models. The rotors generated by the agent were evaluated by computational fluid dynamic methods, and the flow field analysis indicated that the combination of the sweep, lean, and segment angle modifications reduced the loss near the tip, while improving the pressure ratio in the middle section. Different policy combinations were explored, confirming that the combined policy improved the rotor performance more than single policies. The results demonstrate that the proposed RL method can guide future compressor designs.
Seed Priming Treatments to Improve Heat Stress Tolerance of Garden Pea (Pisum sativum L.)
Authors: Tamindžić Gordana , Ignjatov Maja , Miljaković Dragana , Červenski Janko , Milošević Dragana , Nikolić Zorica , Vasiljević Sanja
Published: February 2023

Heat stress seriously affects the production of cool-season food legume crops such as garden peas. Seed priming is a widely used technique that increases germination and improves plant growth and development, resulting in better field performance and higher yield of crops. In the current study, we investigated three seed priming treatments—hydropriming (dH2O), osmopriming (2.2% w/v CaCl2), and hormopriming (50 mg L−1 salicylic acid—SA)—and their effect on germination, initial seedling development, and physiological traits of two novel garden pea cultivars, under optimal conditions and heat stress. Seed priming with H2O, CaCl2, and SA enhanced garden pea performance under both optimal and stress conditions via significant improvements in germination energy, final germination, mean germination time, mean germination rate, seedling vigor index, shoot length, root length, fresh seedling weight, dry seedling weight, shoot elongation rate, root elongation rate, relative water content, chlorophyll content, and membrane stability index, as compared to control. The highest effect on the examined parameters was achieved by osmopriming and hormopriming in both cultivars, suggesting that these treatments could be used to improve the heat stress tolerance of garden pea, after extensive field trials.
Non-Affinity Purification of Antibodies
Authors: Arakawa Tsutomu , Tomioka Yui , Nakagawa Masataka , Sakuma Chiaki , Kurosawa Yasunori , Ejima Daisuke , Tsumoto Kouhei , Akuta Teruo
Published: February 2023

Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale. However, Protein A chromatography has a number of drawbacks, which are summarized in this review. As an alternative, we propose a simple small-scale purification protocol without Protein A that uses novel agarose native gel electrophoresis and protein extraction. For large-scale antibody purification, we suggest mixed-mode chromatography that can in part mimic the properties of Protein A resin, focusing on 4-Mercapto-ethyl-pyridine (MEP) column chromatography.
The Chemical Variability, Nutraceutical Value, and Food-Industry and Cosmetic Applications of Citrus Plants: A Critical Review
Authors: Ben Hsouna Anis , Sadaka Carmen , Generalić Mekinić Ivana , Garzoli Stefania , Švarc-Gajić Jaroslava , Rodrigues Francisca , Morais Simone , Moreira Manuela M. , Ferreira Eduarda , Spigno Giorgia , Brezo-Borjan Tanja , Akacha Boutheina Ben , Saad Rania Ben , Delerue-Matos Cristina , Mnif Wissem
Published: February 2023

Citrus fruits occupy an important position in the context of the fruit trade, considering that both fresh fruits and processed products are produced on a large scale. Citrus fruits are recognized as an essential component of the human diet, thanks to their high content of beneficial nutrients such as vitamins, minerals, terpenes, flavonoids, coumarins and dietary fibers. Among these, a wide range of positive biological activities are attributed to terpenes and flavonoids derivatives. In this review, a list of bibliographic reports (from 2015 onwards) on the phytochemical composition, beneficial effects and potential applications of citrus fruits and their by-products is systematically summarized. In detail, information regarding the nutraceutical and medicinal value closely linked to the presence of numerous bioactive metabolites and their growing use in the food industry and food packaging, also considering any technological strategies such as encapsulation to guarantee their stability over time, were evaluated. In addition, since citrus fruit, as well as its by-products, are interesting alternatives for the reformulation of natural cosmetic products, the sector of the cosmetic industry is also explored. More in-depth knowledge of the latest information in this field will contribute to future conscious use of citrus fruits.
Bond Behavior between High-Strength Rebar and Steel-Fiber-Reinforced Concrete under the Influence of the Fraction of Steel Fiber by Volume and High Temperature
Authors: Li Xiaodong , Lu Chengdong , Cui Yifei , Zhou Lichen , Zheng Li
Published: February 2023

Steel-fiber-reinforced concrete (SFRC) is a composite material made by randomly distributing short steel fibers in normal concrete (NC). In this study, central pull-out tests of 32 specimens were performed to investigate the bond behavior between high-strength rebar and SFRC under the influence of the fraction of steel fiber by volume (Vf = 0%, 0.5%, 1.0% and 1.5%) and temperature (T = 20, 200, 400 and 600 °C). The results show that in NC specimens, splitting failure occurs below 400 °C, while split-pullout failure occurs above 600 °C. Split-pullout failure occurs in all SFRC specimens at each tested temperature. The bond strength between rebar and SFRC was found to decay significantly between 400 and 600 °C. The effect of Vf on the improvement in bond strength was more obvious between 400 and 600 °C than between 20 and 400 °C. The positive contribution of steel fibers to bond behavior is the construction of a rigid skeleton with coarse aggregates that can play a bridging role and effectively retard the expansion of concrete cracks. This improves the bond strength between rebar and SFRC at high temperatures. The bond–slip curve can be divided into five stages, namely the initial micro-slide phase, slip phase, splitting failure phase, stress drop phase and residual pull-out phase. A model of the bond–slip relationship between rebar and SFRC considering temperature and Vf was developed by modifying the existing model of the bond–slip relationship between rebar and NC. The model calculation results agree well with those of testing.
A Case of Interdisciplinary Fusion under Dual Carbon Goal: Coordinated Carbon Reduction with Greenhouse Photovoltaics and Electric Vehicles
Authors: Wu Juai , Deng Shiyang , Zhu Yuanmeng , Liu Yun , Wu Yang Andrew , Fu Rong , Hao Sipeng
Published: February 2023

Building a new type of power system is an important guarantee to support China’s “dual carbon” goal. Due to the inseparable relationship between industrial and agricultural production and electric energy utilization, there must be interdisciplinary integration to achieve the goal of “dual carbon”. The disciplines of horticulture and electric power are taken as examples in this paper to analyze the feasibility of carbon emission reduction through coordinating agricultural photovoltaic (PV) greenhouse and electric vehicle (EV) energy storage. Firstly, the mechanism of carbon emission difference caused by electric energy supplementing during EV charging is analyzed. Secondly, in the context of the contradiction between the reduction of battery life caused by discharging (increasing carbon emission) and the increase in PV output consumption by orderly charging and discharging (reducing carbon emission), an optimization model for the synergistic operation of EV clusters and greenhouse PVs (with the objective of minimizing carbon emissions) is proposed. Finally, the effectiveness of the proposed model is verified through simulation cases. The energy storage characteristics of EVs is capable of realizing the transfer of PV power generation in the time dimension, and the coordinated operation of greenhouse PVs and EVs’ charging and discharging can effectively reduce carbon emission during the EV operation period. In a typical summer scenario of PV output, the carbon emission of EVs in V2G (vehicle to grid) mode was reduced by 69.13% compared to disorderly charging. It is shown that the adequacy of PV generation and the orderly dispatching of the charging and discharging of EVs are the key factors in reducing carbon emission throughout the life cycle of EVs.
Experimental and Numerical Investigations of Fracture Behavior for Transversely Isotropic Slate Using Semi-Circular Bend Method
Authors: Li Erqiang , Wei Yanqing , Chen Zhanyang , Zhang Longfei
Published: February 2023

Slate with inherently transverse isotropy is abundant in metamorphic rock, in buildings, and in geotechnical engineering worldwide; the tensile and shear fracture behavior of layered slate is vital to know for engineering applications. In this paper, the Brazilian and semi-circular bend (SCB) tests of layered slate were performed. The fracture characteristics of the slate were investigated by numerical simulations developed by the hybrid finite and cohesive element method (FCEM). Results showed that the measured experimental tensile strength, and mode I fracture toughness of layered slate all showed a typical V-type trend as the bedding angle increased from 0° to 90°, and with divider type. The developed empirical relationship between tensile fracture toughness and tensile strength KIC = 0.094σt + 0.036 fitted experimentally and strongly correlated. The mechanical response and fracture patterns predicted by FCEM agreed well with those of the laboratory experiments. Moreover, the shear fracture behavior and mode II fracture toughness of the layered slate were explored by systematic numerical simulations. Research results provide potential insights for further prediction and improvement of the complex fracture behavior of anisotropic rock masses for rock engineering.
Effects of Configuration Mode on the Light-Response Characteristics and Dry Matter Accumulation of Cotton under Jujube–Cotton Intercropping
Authors: Li Tiantian , Wang Peijuan , Li Yanfang , Li Ling , Kong Ruiya , Fan Wenxia , Yin Wen , Fan Zhilong , Wu Quanzhong , Zhai Yunlong , Chen Guodong , Wan Sumei
Published: February 2023

The current study evaluated the canopy cover competition for light and heat in a jujube–cotton intercropping system to measure the growth and yield performance of cotton, and the optimal cotton planting configuration. In this study, a two-year field experiment (2020 and 2021) was studied with different spacing configuration modes designed as follows: two rows of cotton (CM1) planted 1.4 m apart, four rows of cotton (CM2) planted 1.0 m apart, and six rows of cotton (CM3) planted 0.5 m apart, spacing intercropped jujube trees, respectively. The control treatment consisted of monocultured cotton (CK). The light-response curve was plotted using an LI-6400 XT photosynthesis instrument. Based on the modified rectangular hyperbola model, the photosynthetic characteristics were fitted, and the dry matter distribution characteristics and yield were compared. The results showed that with the increase in photosynthetically active radiation, the net photosynthetic rate (Pn) of each growth phase decreased first and then increased rapidly in the range of 0–200 μmol·m−2·s−1 and then decreased slightly after the inflection point (light saturation point). The light-response curves of stomatal conductance and transpiration rate showed a linear relationship. The trend in the intercellular CO2 concentration response curve was opposite to that of Pn. The maximum Pn (Pmax) of intercropped cotton was significantly impacted by configuration modes, of which CM2 treatment generated 1.8% and 22.8% higher Pmax than the CM1 and CM3 treatments. The cotton yield in the two years ranked as CK > CM3 > CM2 > CM1, and the average land equivalent ratio of CM2 was significantly higher than that of CM3 (22.4%) and CM1 (95.9%). The six-row configuration resulted in greater competition with the trees, which affected the accumulation of below-ground dry matter, while the four-row configuration formed a reasonable canopy structure, which ensured that more photosynthetic substances were distributed to the generative organs. The reasonable four-rows configuration mode may improve the photosynthetic efficiency of intercropped cotton economic yield.
Investigating the Welding Parameters in Friction Stir Welding of Yellow Brass 405-20
Authors: Raza Syed Farhan , Amjad Muhammad , Habib Muhammad Salman , Ahmed Naveed , Riaz Fahid
Published: February 2023

This research presents the numerical and empirical efforts to investigate the effect of friction stir welding (FSW) parameters on the weld temperature, weld strength, and weld hardness for novel brass known as yellow brass 405-20. The numerical approaches used to measure the weld temperature and weld strength were studied for the first time for yellow brass 405-20 and their validations via empirical studies. Two numerical models were simulated including transient thermal analysis and static structural analysis. Thermal distribution leading to maximum weld temperature during FSW of yellow brass was investigated via both simulations and experiments. Moreover, the ultimate tensile strength, namely the weld strength, was measured numerically and validated from its empirical counterpart. Finally, weld hardness was measured empirically to explore the joint health. A maximum temperature of 598 °C was recorded, which was much below the melting point of brass. Joint strength of 228 MPa was observed, which is 83% of the base brass strength. Microscopic examination of the weldment revealed the underlying mechanisms of less weld strength as compared to the parent brass material strength.