bluuebunny/crossref_metadata_2025 huggingface dataset:
MC-ViT: Multi-path cross-scale vision transformer for thymoma histopathology whole slide image typing
Authors: Zhang Huaqi , Chen Huang , Qin Jin , Wang Bei , Ma Guolin , Wang Pengyu , Zhong Dingrong , Liu Jie
Published: October 2022

Objectives

Accurate histological typing plays an important role in diagnosing thymoma or thymic carcinoma (TC) and predicting the corresponding prognosis. In this paper, we develop and validate a deep learning-based thymoma typing method for hematoxylin & eosin (H&E)-stained whole slide images (WSIs), which provides useful histopathology information from patients to assist doctors for better diagnosing thymoma or TC.

Methods

We propose a multi-path cross-scale vision transformer (MC-ViT), which first uses the cross attentive scale-aware transformer (CAST) to classify the pathological information related to thymoma, and then uses such pathological information priors to assist the WSIs transformer (WT) for thymoma typing. To make full use of the multi-scale (10×, 20×, and 40×) information inherent in a WSI, CAST not only employs parallel multi-path to capture different receptive field features from multi-scale WSI inputs, but also introduces the cross-correlation attention module (CAM) to aggregate multi-scale features to achieve cross-scale spatial information complementarity. After that, WT can effectively convert full-scale WSIs into 1D feature matrices with pathological information labels to improve the efficiency and accuracy of thymoma typing.

Results

We construct a large-scale thymoma histopathology WSI (THW) dataset and annotate corresponding pathological information and thymoma typing labels. The proposed MC-ViT achieves the Top-1 accuracy of 0.939 and 0.951 in pathological information classification and thymoma typing, respectively. Moreover, the quantitative and statistical experiments on the THW dataset also demonstrate that our pipeline performs favorably against the existing classical convolutional neural networks, vision transformers, and deep learning-based medical image classification methods.

Conclusion

This paper demonstrates that comprehensively utilizing the pathological information contained in multi-scale WSIs is feasible for thymoma typing and achieves clinically acceptable performance. Specifically, the proposed MC-ViT can well predict pathological information classes as well as thymoma types, which show the application potential to the diagnosis of thymoma and TC and may assist doctors in improving diagnosis efficiency and accuracy.
Thymic cyst: Is attenuation artifactually increased on contrast-enhanced CT?
Authors: Wang Chao , Mao Jin , Yang Siyu , Xie Huanhuan , Wang Shan , Hu Ling
Published: October 2022

Background

Thymic cysts are often misinterpreted as thymomas or lymph nodes, then leading to unnecessary thymectomy. The purpose of this study was to investigate how the adjacent large vessels artifactually affected attenuation values of thymic cysts on contrast–enhanced CT (CE-CT).

Methods

In this retrospective study, a total of 84 patients were included with pathological diagnosis of thymic cysts and preoperative CE-CT. Quantitative measurements of the size, CT attenuation of thymic cysts and CT attenuation of adjacent large vessels were performed on preoperative CE-CT. According to the absolute change in attenuation of the cysts between contrast-enhanced and nonenhanced CT, the patients were classified into the groups of artifactual hyper-density, unchanged density, and artifactual hypo-density. CT characteristics were compared between the three groups. Furthermore, multivariable logistic regression analysis was performed to determine the independent factors for artifactual hyper-density.

Results

The group of artifactual hyper-density had smaller short diameter of the cysts, higher postcontrast attenuation values and lower nonenhanced attenuation values of the adjacent large vessel. Furthermore, the multivariable logistic analysis showed that artifactual hyper-density of thymic cysts was negatively associated with nonenhanced attenuation of adjacent large vessel, and positively associated with postcontrast attenuation of adjacent large vessel and postcontrast attenuation of cysts.

Conclusions

Most cases with >20 HU nonenhanced CT attenuation in surgically resected cases. Artifactual hyper-density─pseudo-enhancement phenomenon of thymic cysts was more apparent in higher increasing attenuation of adjacent large vessels on CE-CT. A well understanding of this phenomenon can help reduce preoperative misdiagnosis and unnecessary thymectomy.
Acute necrotizing encephalopathy associated with lymphoma-associated hemophagocytic lymphohistiocytosis: A case report and literature review
Authors: Sun Wenqiang , Fu Changchang , Zhu Xueping
Published: October 2022

Damage associated with lymphoma-associated hemophagocytic lymphohistiocytosis (LA-HLH) to the central nervous system (CNS) is not uncommon. However, the combination with brain damage resembling acute necrotizing encephalopathy (ANE) is rarely reported. Herein, we introduce the diagnosis and treatment of a case of ANE associated with LA-HLH in our hospital and review the relevant literature. After treatment, the child was discharged with only dysarthria and decreased sucking ability. The child is now discharged from the hospital for 6 months with regular follow-up. There were no disease recurrence signs. LA-HLH and ANE were related to cytokine storm. Therefore, early steroid application is essential for treating these diseases.
Innovations and adaptations in neonatal and pediatric respiratory care for resource constrained settings
Authors: Wu Andrew , Mukhtar-Yola Mariya , Luch Sreyleak , John Stephen , Adhikari Bikash Raj , Bakker Caitlin , Slusher Tina , Bjorklund Ashley , Winter Jameel , Ezeaka Chinyere
Published: October 2022

Respiratory disease is a leading cause of death in children under 5 years of age worldwide, and most of these deaths occur in low- to middle-income countries (LMICs) where advanced respiratory care technology is often limited. Much of the equipment required to provide advanced respiratory care is unavailable in these areas due to high costs, the need for specialty trained personnel, and myriad other resource constraints that limit uptake and sustainable use of these devices, including reliable access to electricity, sensitive equipment needing frequent maintenance, single-patient-use supplies, and lack of access to sterilization equipment. Compounding the problem, pediatrics is uniquely challenging in that one size does not fit all, or even most patients. Despite these substantial barriers, numerous innovations in respiratory care technology have been made in recent years that have brought increasing access to high quality respiratory care in some of the most remote areas of the world. In this article, we intend to review the global burden of respiratory diseases for children, highlight the prototypical innovations that have been made in bringing respiratory care to LMICs, spotlight some of the technologies being actively developed to improve respiratory care in resource-constrained settings, and conclude with a discussion highlighting areas where further innovation is still needed.
Viscum album mother tinctures: Harvest conditions and host trees influence the plant metabolome and the glycolytic pathway of breast cancer cells
Authors: Melo Michelle Nonato de Oliveira , Ochioni Alan Clavelland , Zancan Patricia , Oliveira Adriana Passos , Grazi Mirio , Garrett Rafael , Holandino Carla , Baumgartner Stephan
Published: October 2022

Viscum album is a semi-parasitic plant used for over one hundred years in complementary cancer therapy. The main commercial drugs used in cancer patients’ treatment are derived from the aqueous V. album extracts, whose cytotoxic potential is mostly attributed to the aqueous soluble antitumoral metabolites. On the counterpart, ethanol solvents must be used to obtain V. album mother tinctures. This methodology permits better solubilization of phenolic compounds, among others, which present antitumoral bioactivity. Recently, the metabolomics approach revealed the influence of the host tree on the V. album subspecies differentiation. To increase the scientific information about the chemical differences related to the host trees and to clarify the seasonal influences, in this study, the metabolome of 50 V. album mother tinctures from three subspecies (abietis, album, austriacum) and five host trees (Malus domestica, Quercus sp., Ulmus carpinifolia, Pinus sylvestris, Abies alba) was evaluated using summer and winter plant harvests. The in vitro cytotoxic activities were investigated in breast cancer cells (MDA-MB-231) and immortalized normal human keratinocytes (HaCaT). The summer V. album mother tinctures presented higher cytotoxic activity than winter ones. Among the summer samples, those prepared with V. album subsp. album were more cytotoxic than V. album subsp. abietis and subsp. V. album subsp. austriacum. The V. album harvested from Quercus petraea and Abies alba inhibited the key-glycolytic enzymes: hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK). This activity was related to a reduction in glucose uptake and lactate production, which were host-tree-time-dose-dependent. The untargeted metabolomic approach was able to discriminate the mother tinctures according to respective botanical classes and harvest season. A total of 188 metabolites were annotated under positive and negative modes. Fourteen compounds were responsible for the samples differentiation, and, to the best of our knowledge, eight were described in the Viscum album species for the first time. Our study shows the interruption of the Warburg effect as a novel antitumoral mechanism triggered by V. album mother tinctures, which is related to their metabolite profile. These results bring scientific evidence that encourages the use of V. album mother tinctures as a natural product for cancer therapy.
Anxiolytic effect of YangshenDingzhi granules: Integrated network pharmacology and hippocampal metabolomics
Authors: Lv Shimeng , Dai Weibo , Zheng Yan , Dong Ping , Yu Yihong , Zhao Yifan , Sun Shiguang , Bi Dezhong , Liu Chuanguo , Han Fabin , Wu Jibiao , Zhao Tingting , Ma Yuexiang , Zheng Feng , Sun Peng
Published: October 2022

Anxiety disorder is one of the most common mental diseases. It is mainly characterized by a sudden, recurring but indescribable panic, fear, tension and/or anxiety. Yangshendingzhi granules (YSDZ) are widely used in the treatment of anxiety disorders, but its active ingredients and underlying mechanisms are not yet clear. This study integrates network pharmacology and metabolomics to investigate the potential mechanism of action of YSDZ in a rat model of anxiety. First, potential active ingredients and targets were screened by network pharmacology. Then, predictions were verified by molecular docking, molecular dynamics and western blotting. Metabolomics was used to identify differential metabolites and metabolic pathways. All results were integrated for a comprehensive analysis. Network pharmacology analysis found that Carotene, β-sitosterol, quercetin, Stigmasterol, and kaempferol in YSDZ exert anxiolytic effects mainly by acting on IL1β, GABRA1, PTGS1, ESR1, and TNF targets. Molecular docking results showed that all the affinities were lower than −5 kcal/mol, and the average affinities were −7.7764 kcal/mol. Molecular dynamics simulation results showed that RMSD was lower than 2.5 A, and the overall conformational changes of proteins were small, indicating that the small molecules formed stable complexes with proteins. The results of animal experiments showed that YSDZ exerts anxiolytic effects by regulating GABRA1 and TNF-α, ameliorating pathological damage in hippocampal CA1, and regulating metabolic pathways such as thiamine, cysteine and methionine metabolism, lysine biosynthesis and degradation. Altogether, we reveal multiple mechanisms through which YSDZ exerts its anti-anxiety effects, which may provide a reference for its clinical application and drug development.
Peripheral proteomic changes after electroconvulsive seizures in a rodent model of non-response to chronic fluoxetine
Authors: Lebeau Rodolphe H. , Mendez-David Indira , Kucynski-Noyau Laura , Henry Céline , Attali David , Plaze Marion , Colle Romain , Corruble Emmanuelle , Gardier Alain M. , Gaillard Raphaël , Guilloux Jean-Philippe , David Denis J.
Published: October 2022

Major depressive disorder (MDD) is the psychiatric disorder with the highest prevalence in the world. Pharmacological antidepressant treatment (AD), such as selective serotonin reuptake inhibitors [SSRI, i.e., fluoxetine (Flx)] is the first line of treatment for MDD. Despite its efficacy, lack of AD response occurs in numerous patients characterizing Difficult-to-treat Depression. ElectroConvulsive Therapy (ECT) is a highly effective treatment inducing rapid improvement in depressive symptoms and high remission rates of ∼50–63% in patients with pharmaco-resistant depression. Nevertheless, the need to develop reliable treatment response predictors to guide personalized AD strategies and supplement clinical observation is becoming a pressing clinical objective. Here, we propose to establish a proteomic peripheral biomarkers signature of ECT response in an anxio/depressive animal model of non-response to AD. Using an emotionality score based on the analysis complementary behavioral tests of anxiety/depression (Elevated Plus Maze, Novelty Suppressed Feeding, Splash Test), we showed that a 4-week corticosterone treatment (35 μg/ml, Cort model) in C57BL/6JRj male mice induced an anxiety/depressive-like behavior. A 28-day chronic fluoxetine treatment (Flx, 18 mg/kg/day) reduced corticosterone-induced increase in emotional behavior. A 50% decrease in emotionality score threshold before and after Flx, was used to separate Flx-responding mice (Flx-R, n = 18), or Flx non-responder mice (Flx-NR, n = 7). Then, Flx-NR mice received seven sessions of electroconvulsive seizure (ECS, equivalent to ECT in humans) and blood was collected before and after ECS treatment. Chronic ECS normalized the elevated emotionality observed in Flx-NR mice. Then, proteins were extracted from peripheral blood mononuclear cells (PBMCs) and isolated for proteomic analysis using a high-resolution MS Orbitrap. Data are available via ProteomeXchange with identifier PXD037392. The proteomic analysis revealed a signature of 33 peripheral proteins associated with response to ECS (7 down and 26 upregulated). These proteins were previously associated with mental disorders and involved in regulating pathways which participate to the depressive disorder etiology.
Modeling geomagnetic induction in submarine cables
Authors: Chakraborty Shibaji , Boteler David H. , Shi Xueling , Murphy Benjamin S. , Hartinger Michael D. , Wang Xuan , Lucas Greg , Baker Joseph B. H.
Published: October 2022

Submarine cables have become a vital component of modern infrastructure, but past submarine cable natural hazard studies have mostly focused on potential cable damage from landslides and tsunamis. A handful of studies examine the possibility of space weather effects in submarine cables. The main purpose of this study is to develop a computational model, using Python, of geomagnetic induction on submarine cables. The model is used to estimate the induced voltage in the submarine cables in response to geomagnetic disturbances. It also utilizes newly acquired knowledge from magnetotelluric studies and associated investigations of geomagnetically induced currents in power systems. We describe the Python-based software, its working principle, inputs/outputs based on synthetic geomagnetic field data, and compare its operational capabilities against analytical solutions. We present the results for different model inputs, and find: 1) the seawater layer acts as a shield in the induction process: the greater the ocean depth, the smaller the seafloor geoelectric field; and 2) the model is sensitive to the Ocean-Earth layered conductivity structure.
Altered contractility in mutation-specific hypertrophic cardiomyopathy: A mechano-energetic in silico study with pharmacological insights
Authors: Forouzandehmehr Mohamadamin , Paci Michelangelo , Koivumäki Jussi T , Hyttinen Jari
Published: October 2022

Introduction: Mavacamten (MAVA), Blebbistatin (BLEB), and Omecamtiv mecarbil (OM) are promising drugs directly targeting sarcomere dynamics, with demonstrated efficacy against hypertrophic cardiomyopathy (HCM) in (pre)clinical trials. However, the molecular mechanism affecting cardiac contractility regulation, and the diseased cell mechano-energetics are not fully understood yet.

Methods: We present a new metabolite-sensitive computational model of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) electromechanics to investigate the pathology of R403Q HCM mutation and the effect of MAVA, BLEB, and OM on the cell mechano-energetics.

Results: We offer a mechano-energetic HCM calibration of the model, capturing the prolonged contractile relaxation due to R403Q mutation (∼33%), without assuming any further modifications such as an additional Ca2+ flux to the thin filaments. The HCM model variant correctly predicts the negligible alteration in ATPase activity in R403Q HCM condition compared to normal hiPSC-CMs. The simulated inotropic effects of MAVA, OM, and BLEB, along with the ATPase activities in the control and HCM model variant agree with in vitro results from different labs. The proposed model recapitulates the tension-Ca2+ relationship and action potential duration change due to 1 µM OM and 5 µM BLEB, consistently with in vitro data. Finally, our model replicates the experimental dose-dependent effect of OM and BLEB on the normalized isometric tension.

Conclusion: This work is a step toward deep-phenotyping the mutation-specific HCM pathophysiology, manifesting as altered interfilament kinetics. Accordingly, the modeling efforts lend original insights into the MAVA, BLEB, and OM contributions to a new interfilament balance resulting in a cardioprotective effect.
The Lognormal Lung: A new approach to quantifying lung inhomogeneity in COPD
Authors: Smith Nicholas M. J. , Magor-Elliott Snapper R. M. , Fullerton Christopher J. , Couper John H. , Richmond Graham , Hancock Gus , Ritchie Grant A. D. , Robbins Peter A. , Talbot Nick P. , Petousi Nayia
Published: October 2022

Early diagnosis and disease phenotyping in COPD are currently limited by the use of spirometry, which may remain normal despite significant small-airways disease and which may not fully capture a patient’s underlying pathophysiology. In this study we explored the use of a new non-invasive technique that assesses gas-exchange inhomogeneity in patients with COPD of varying disease severity (according to GOLD Stage), compared with age-matched healthy controls. The technique, which combines highly accurate measurement of respiratory gas exchange using a bespoke molecular flow sensor and a mechanistic mathematical model of the lung, provides new indices of lung function: the parameters σCL, σCd, and σVD represent the standard deviations of distributions for alveolar compliance, anatomical deadspace and vascular conductance relative to lung volume, respectively. It also provides parameter estimates for total anatomical deadspace and functional residual capacity (FRC). We demonstrate that these parameters are robust and sensitive, and that they can distinguish between healthy individuals and those with mild-moderate COPD (stage 1–2), as well as distinguish between mild-moderate COPD (stage 1–2) and more severe (stage 3–4) COPD. In particular, σCL, a measure of unevenness in lung inflation/deflation, could represent a more sensitive non-invasive marker of early or mild COPD. In addition, by providing a multi-dimensional assessment of lung physiology, this technique may also give insight into the underlying pathophysiological phenotype for individual patients. These preliminary results warrant further investigation in larger clinical research studies, including interventional trials.
RETRACTED: Interaction between zinc and selenium bio-fortification and toxic metals (loid) accumulation in food crops
Authors: Bayanati Mina , Al-Tawaha Abdel Rahman , Al-Taey Duraid , Al-Ghzawi Abdul Latief , Abu-Zaitoon Yousef M. , Shawaqfeh Samar , Al-Zoubi Omar , Al-Ramamneh Ezz Al-Dein , Alomari Laith , Al-Tawaha Abdel Razzaq , Dey Abhijit
Published: October 2022

Biofortification is the supply of micronutrients required for humans and livestock by various methods in the field, which include both farming and breeding methods and are referred to as short-term and long-term solutions, respectively. The presence of essential and non-essential elements in the atmosphere, soil, and water in large quantities can cause serious problems for living organisms. Knowledge about plant interactions with toxic metals such as cadmium (Cd), mercury (Hg), nickel (Ni), and lead (Pb), is not only important for a healthy environment, but also for reducing the risks of metals entering the food chain. Biofortification of zinc (Zn) and selenium (Se) is very significant in reducing the effects of toxic metals, especially on major food chain products such as wheat and rice. The findings show that Zn- biofortification by transgenic technique has reduced the accumulation of Cd in shoots and grains of rice, and also increased Se levels lead to the formation of insoluble complexes with Hg and Cd. We have highlighted the role of Se and Zn in the reaction to toxic metals and the importance of modifying their levels in improving dietary micronutrients. In addition, cultivar selection is an essential step that should be considered not only to maintain but also to improve the efficiency of Zn and Se use, which should be considered more climate, soil type, organic matter content, and inherent soil fertility. Also, in this review, the role of medicinal plants in the accumulation of heavy metals has been mentioned, and these plants can be considered in line with programs to improve biological enrichment, on the other hand, metallothioneins genes can be used in the program biofortification as grantors of resistance to heavy metals.
Integrated metabolome and transcriptome analysis reveals salicylic acid and flavonoid pathways’ key roles in cabbage’s defense responses to Xanthomonas campestris pv. campestris
Authors: Sun Qingguo , Xu Zhongmin , Huang Wei , Li Dawei , Zeng Qi , Chen Lin , Li Baohua , Zhang Enhui
Published: October 2022

Xanthomonas campestris pv. campestris (Xcc) is a vascular bacteria pathogen causing black rot in cabbage. Here, the resistance mechanisms of cabbage against Xcc infection were explored by integrated metabolome and transcriptome analysis. Pathogen perception, hormone metabolisms, sugar metabolisms, and phenylpropanoid metabolisms in cabbage were systemically re-programmed at both transcriptional and metabolic levels after Xcc infection. Notably, the salicylic acid (SA) metabolism pathway was highly enriched in resistant lines following Xcc infection, indicating that the SA metabolism pathway may positively regulate the resistance of Xcc. Moreover, we also validated our hypothesis by showing that the flavonoid pathway metabolites chlorogenic acid and caffeic acid could effectively inhibit the growth of Xcc. These findings provide valuable insights and resource datasets for further exploring Xcc–cabbage interactions and help uncover molecular breeding targets for black rot-resistant varieties in cabbage.
Weighted gene co-expression network analysis reveals key module and hub genes associated with the anthocyanin biosynthesis in maize pericarp
Authors: Li Tingchun , Wang Yiting , Dong Qing , Wang Fang , Kong Fanna , Liu Guihu , Lei Yanli , Yang Huaying , Zhou Yingbing , Li Cheng
Published: October 2022

Anthocyanins are the visual pigments that present most of the colors in plants. Its biosynthesis requires the coordinated expression of structural genes and regulatory genes. Pericarps are the rich sources of anthocyanins in maize seeds. In the experiment, the transcriptomes of transparent and anthocyanins-enriched pericarps at 15, 20, and 25 DAP were obtained. The results output 110.007 million raw reads and 51407 genes’ expression matrix. Using data filtration in R language, 2057 genes were eventually identified for weighted gene co-expression network analysis. The results showed that 2057 genes were classified into ten modules. The cyan module containing 183 genes was confirmed to be the key module with the highest correlation value of 0.98 to the anthocyanins trait. Among 183 genes, seven structural genes were mapped the flavonoid biosynthesis pathway, and a transcription factor Lc gene was annotated as an anthocyanin regulatory gene. Cluster heatmap and gene network analysis further demonstrated that Naringenin, 2-oxoglutarate 3-dioxygenase (Zm00001d001960), Dihydroflavonol 4-reductase (Zm00001d044122), Leucoanthocyanidin dioxygenase (Zm00001d014914), anthocyanin regulatory Lc gene (Zm00001d026147), and Chalcone synthase C2 (Zm00001d052673) participated in the anthocyanins biosynthesis. And the transcription factor anthocyanin regulatory Lc gene Zm00001d026147 may act on the genes Chalcone synthase C2 (Zm00001d052673) and Dihydroflavonol 4-reductase (Zm00001d044122). The yeast one-hybrid assays confirmed that the Lc protein could combine with the promoter region of C2 and directly regulate the anthocyanin biosynthesis in the pericarp. These results may provide a new sight to uncover the module and hub genes related to anthocyanins biosynthesis in plants.
Role of reactive oxygen species in lesion mimic formation and conferred basal resistance to Fusarium graminearum in barley lesion mimic mutant 5386
Authors: Wang Wenqiang , Zhang Jifa , Guo Fenxia , Di Yindi , Wang Yuhui , Li Wankun , Sun Yali , Wang Yuhai , Ni Fei , Fu Daolin , Wang Wei , Hao Qunqun
Published: October 2022

This study investigated the barley lesion mimic mutant (LMM) 5386, evidenced by a leaf brown spot phenotype localized on the chromosome 3H, and its conferred basal resistance to Fusarium graminearum. RNA-seq analysis identified 1453 genes that were differentially expressed in LMM 5386 compared to those in the wild type. GO and KEGG functional annotations suggested that lesion mimic formation was mediated by pathways involving oxidation reduction and glutathione metabolism. Additionally, reactive oxygen species (ROS) accumulation in brown spots was substantially higher in LMM 5386 than in the wild-type plant; therefore, antioxidant competence, which is indicated by ROS accumulation, was significantly lower in LMM 5386. Furthermore, the reduction of glycine in LMM 5386 inhibited glutathione biosynthesis. These results suggest that the decrease in antioxidant competence and glutathione biosynthesis caused considerable ROS accumulation, leading to programmed cell death, which eventually reduced the yield components in LMM 5386.
The protein kinase FvRIPK1 regulates plant morphogenesis by ABA signaling using seed genetic transformation in strawberry
Authors: Chen Xuexue , Gu Xiaojiao , Gao Fan , Guo Jiaxuan , Shen Yuanyue
Published: October 2022

A strawberry RIPK1, a leu-rich repeat receptor-like protein kinase, is previously demonstrated to be involved in fruit ripening as a positive regulator; however, its role in vegetable growth remains unknown. Here, based on our first establishment of Agrobacterium-mediated transformation of germinating seeds in diploid strawberry by FvCHLH/FvABAR, a reporter gene that functioned in chlorophyll biosynthesis, we got FvRIPK1-RNAi mutants. Downregulation of FvRIPK1 inhibited plant morphogenesis, showing curled leaves; also, this silencing significantly reduced FvABAR and FvABI1 transcripts and promoted FvABI4, FvSnRK2.2, and FvSnRK2.6 transcripts. Interestingly, the downregulation of the FvCHLH/ABAR expression could not affect FvRIPK1 transcripts but remarkably reduced FvABI1 transcripts and promoted FvABI4, FvSnRK2.2, and FvSnRK2.6 transcripts in the contrast of the non-transgenic plants to the FvCHLH/FvABAR-RNAi plants, in which chlorophyll contents were not affected but had abscisic acid (ABA) response in stomata movement and drought stress. The distinct expression level of FvABI1 and FvABI4, together with the similar expression level of FvSnRK2.2 and FvSnRK2.6 in the FvRIPK1- and FvABAR/CHLH-RNAi plants, suggested that FvRIPK1 regulated plant morphogenesis probably by ABA signaling. In addition, FvRIPK1 interacted with FvSnRK2.6 and phosphorylated each other, thus forming the FvRIPK1–FvSnRK2.6 complex. In conclusion, our results provide new insights into the molecular mechanism of FvRIPK1 in plant growth.
Multi-peril pathogen risks to global wheat production: A probabilistic loss and investment assessment
Authors: Chai Yuan , Senay Senait , Horvath Diana , Pardey Philip
Published: October 2022

Crop diseases cause significant food and economic losses. We examined the joint, probabilistic, long-term, bio-economic impact of five major fungal pathogens of wheat on global wheat production by combining spatialized estimates of their climate suitability with global wheat production and modeled distributions of potential crop losses. We determined that almost 90% of the global wheat area is at risk from at least one of these fungal diseases, and that the recurring losses attributable to this set of fungal diseases are upwards of 62 million tons of wheat production per year. Our high-loss regime translates to around 8.5% of the world’s wheat production on average—representing calories sufficient to feed up to 173 million people each year. We estimate that a worldwide research expenditure of $350-$974 million (2018 prices) annually on these five fungal diseases of wheat, let alone other pathogens, can be economically justified, equivalent to 2 to 5 times more than the amount we estimate is currently spent onallwheat disease-related public R&D.
More linear than log? Non-symbolic number-line estimation in 3- to 5-year-old children
Authors: Haman Maciej , Patro Katarzyna
Published: October 2022

The number-line estimation task has become one of the most important methods in numerical cognition research. Originally applied as a direct measure of spatial number representation, it became also informative regarding various other aspects of number processing and associated strategies. However, most of this work and associated conclusions concerns processing numbers in a symbolic format, by school children and older subjects. Symbolic number system is formally taught and trained at school, and its basic mathematical properties (e.g., equidistance, ordinality) can easily be transferred into a spatial format of an oriented number line. This triggers the question on basic characteristics of number line estimation before children get fully familiar with the symbolic number system, i.e., when they mostly rely on approximate system for non-symbolic quantities. In our three studies, we examine therefore how preschool children (3–5-years old) estimate position ofnon-symbolicquantities on a line, and how this estimation is related to the developing symbolic number knowledge and cultural (left-to-right) directionality. The children were tested with the Give-a-number task, then they performed a computerized number-line task. In Experiment 1, lines bounded with sets of 1 and 20 elements going left-to-right or right-to-left were used. Even in the least numerically competent group, the linear model better fit the estimates than the logarithmic or cyclic power models. The line direction was irrelevant. In Experiment 2, a 1–9 left-to-right oriented line was used. Advantage of linear model was found at group level, and variance of estimates correlated with tested numerosities. In Experiment 3, a position-to-number procedure again revealed the advantage of the linear model, although the strategy of selecting an option more similar to the closer end of the line was prevalent. The precision of estimation increased with the mastery of counting principles in all three experiments. These results contradict the hypothesis of the log-to-linear shift in development of basic numerical representation, rather supporting the linear model with scalar variance. However, the important question remains whether the number-line task captures the nature of the basic numerical representation, or rather the strategies of mapping that representation to an external space.
Sustainable career and employability of student leaders in China
Authors: Bai Bin , Li Mixue , Lyu Xinshan
Published: October 2022

Research has shown that most employers prefer employing graduates with student leadership experience in colleges and most of the student leaders have sustainable development in their careers in China. Compared with students without leadership experience, student leaders are easier to get promotion and development opportunities in the workplace. To discuss this question, researchers chose employers, student leaders, and teachers as research participants in an effort to answer the question of why employers prefer graduates with experience being student leaders. This study focuses on what key competencies student leaders possess and how their competencies can match the capability requirement of employers. Semi-structured interviews were conducted with 9 outstanding student leaders, 4 class teachers, 2 student affairs administrators, and 8 human resource managers from two vocational colleges in Beijing. We find that student leaders have strong social competence, emotional competence, action competence, responsibility, and resilience which employers value and these competencies are essential to career development in the workplace. These competencies are considered to be helpful to support student leaders to adapt to the real work environment quickly and achieve career success.
Antipsychotic prescribing practices in real-life (APPREAL study): Findings from the French National Healthcare System Database (2007–2017)
Authors: Rolland Benjamin , Dalon Faustine , Gauthier Noémie , Nourredine Mikaïl , Bérard Marjorie , Carton Louise , Brousse Georges , Llorca Pierre-Michel , Jacoud Flore , Van Ganse Eric , Belhassen Manon
Published: October 2022

Background

Antipsychotics are used in a large variety of psychiatric and neurological disorders; investigating their use in real life is important to understand national prescribing practices, as well as to determine the levels of patient adherence.

Methods

Using a 1/97e random sample (General Sample of Beneficiaries, EGB) of the French health insurance reimbursement database, we conducted a historical cohort study on the 2007–2017 period. The aim was to describe the sociodemographic characteristics of patients, the types of antipsychotics dispensed, the types of prescribers, the mean doses and average durations of treatment, the co-dispensed medications, and the levels of adherence to treatment. To exclude punctual uses of antipsychotics, we selected only patients with a continuous dispensing of the same antipsychotic over at least 3 months.

Results

In total, 13,799 subjects (1.66% of the EGB sample) were included (56.0% females; mean age 55.8 ± 19.4 years). Risperidone (19.3%), cyamemazine (18.7%), olanzapine (11.9%), tiapride (8.8%), and haloperidol (7.5%) were the five most prescribed antipsychotics. 44.9% of prescriptions were written by general practitioners, 34.1% by hospital practitioners, and 18.4% by private-practice psychiatrists. On average, the mean dispensed doses were relatively low, but the variation range was large. Long-acting forms were used in 5.4% of the sample, and clozapine in 1.3%. 34.2% of patients received more than one antipsychotic, and almost 15% were prescribed at least three concomitant antipsychotics. Paliperidone and clozapine were associated with the highest levels of adherence, and risperidone and haloperidol with the lowest ones.

Conclusion

An important heterogeneity of antipsychotic prescribing practices was observed in France. The rate of use of long-acting antipsychotics was low, whereas multiple antipsychotic prescriptions were frequent.
A cross-sectional survey exploring the attitude, knowledge, and use of anesthesia teams toward evidence-based practice in Riyadh Saudi Arabia
Authors: Al Anazi Salem Khalaf , Al Zahrani Waleed Abdullah , Alsanad Mohammed Abdulaziz , Alzahrani Matar Saeed , Al Ghamdi Ibrahim Saeed , Alotaibi Abdulmueen Awadh , Al maliki Mohammed Ali , Asiri Hamzah Mohammed , Alshehri Ghirman Mohammed , Alanazi Abdullah Salem , Al Anazi Abdulelah Khalaf
Published: October 2022

Background

Evidence-based practice (EBP) plays a crucial role in improving the quality of healthcare services by ensuring the delivery of the highest and safest level of patient care since EBP helps in justifying treatment choices to patients. Studies that examine the levels of EBP knowledge, attitudes toward EBP, and use of the use of EBP within anesthetic teams' practice are lacking, hence it is necessary to explore this.

Aim

To evaluate anesthesia teams' levels of knowledge, attitude toward and use of the evidence-based practice in a local hospital in Saudi Arabia.

Method

In one hospital, a cross-sectional survey was conducted using a convenience sampling technique using a validated questionnaire instrument called the Evidence-Based Practice EBP Questionnaire. The questionnaire was distributed through an online method to 173 participants. Descriptive and inferential statistics Tests were utilized to analyse the retrieved data using the SPSS program.

Results

One hundred and forty questionnaires were completed and returned, yielding a response rate of 80.9%. Overall, anesthesia teams showed a high positive attitude toward EBP but low levels of knowledge and use of EBP. Participants with higher levels of education and/or work experience exhibited significantly higher levels of knowledge and use of EBP than those who had lower education levels and/or work experience. Also, higher levels of education and/or work experience exhibited a significant positive association toward a higher level of knowledge and use of EBP. However, attitude levels toward EBP did not exhibit either significant or associated. Physicians showed significantly higher knowledge and use of EBP than non-physicians. Lack of knowledge and lack of time due to workload were the leading barriers encountered by anesthesia teams ATs.

Conclusion

Education level, work experience and job position affect the knowledge, attitude, and use of EBP. Continuous education and minimizing barriers are recommended to enhance the knowledge, attitude, and use of EBP among anesthesia teams in Saudi Arabia.
Evaluation of a community intervention program on knowledge, attitudes, and practices regarding severe fever with thrombocytopenia syndrome in Anhui Province, China
Authors: Gong Lei , Zhang Yong , Wang Jinsheng , Xiong Yingying , Wang Jiling , Wu Jiabing , Chen Fang , Zhu Meng , Cheng Donglin , Jiang Xuqin
Published: October 2022

Background

Severe fever with thrombocytopenia syndrome (SFTS) is a novel infectious disease with no specific therapeutics and vaccines. We hypothesize that health education in vulnerable people would ameliorate their knowledge, attitudes and practices (KAP) regarding SFTS and reduce its prevalence.

Methods

A four-stage cluster cross-section study in sixteen community units was performed. Sixteen groups were allocated to the intervention or control groups. A 6 months education program was administrated. The primary outcome was KAP scores 6 months after intervention. Predictors of KAP score changes were also analyzed.

Results

Eight hundred and fifteen valid questionnaires pre-intervention and 767 ones post-intervention were retreated. No significant differences were found in demographic characteristics and KAP scores before intervention. A significant improvement in KAP score (16.8 ± 4.7 vs. 22.0 ± 4.2, p < 0.001) in the intervention group was observed compare with the controls. Educational level and intervention program were the common predictors of KAP score changes.

Conclusions

Education improved KAP scores in SFTS vulnerable people which may contribute to the control of the disease.
Vegan milk and egg alternatives commercialized in Brazil: A study of the nutritional composition and main ingredients
Authors: Romão Bernardo , Botelho Raquel Braz Assunção , Nakano Eduardo Yoshio , Borges Vinícius Ruela Pereira , de Holanda Maria Eduarda Machado , Raposo António , Han Heesup , Gil-Marín Miseldra , Ariza-Montes Antonio , Zandonadi Renata Puppin
Published: October 2022

Worldwide, there is an increasing demand for plant-based food due to sustainable, health, ethical, religious, philosophical, and economic reasons. In Brazil, 14% of the population declares themselves vegetarians, and a noticeable increase in the consumption of vegan products is also noted. Substitution of animal dairy and egg is challenging from both sensory and nutritional aspects. Yet, there are no data regarding the nutritional value and ingredients of Brazilian commercial dairy and egg substitutes. Therefore, this study aimed to analyze the nutritional composition and used ingredients in Brazilian commercial vegan alternatives to dairy and eggs to provide information for Brazilian consumers of these products. A cross-sectional quantitative study was carried out in three steps: (i) sample mapping. (ii) data collection, and (iii) statistical analysis. A total of 152 samples were included. No differences were found between the energy value and total fat of vegan products and their animal counterparts. Vegan products showed higher amounts of carbohydrates and dietary fiber, and only the vegan versions of beverages and cheeses showed less protein than their counterparts. Cashews, rice, coconut, and soy were the most used ingredients in dairy substitutes. Emulsions of oil, starch, and isolated protein were used in vegan egg products. Most vegan beverages presented sugar in their composition. Vegan alternatives of dairy and eggs might be suitable for substituting their animal counterparts, but given that traditional versions of cheeses and milk are sources of protein in omnivorous diets, for equivalent nutritional replacement in vegan products, it is necessary to improve the protein content of their vegan counterparts.
Rare penetrating abdominal injury caused by falling from height: Miraculously good prognosis
Authors: Liu Yanquan , Xu Qinglin , Zhu Hongquan , Wang Jun , Zeng Fanlin , Lin Jie
Published: October 2022

Fall from height causing penetrating abdominal visceral injuries is rare condition leading to abdominal multiorgan damage. It carries high mortality. A case of construction site worker sustaining penetrating visceral injuries by falling from height leading to impalement of steal bar from anus presented to our hospital and managed by timely evacuation from site of accident to hospital, resuscitations, radiological investigations and multi-Disciplinary team management lead to successful outcome. Our protocol for such cases will be discussed with references.
Single-port robotic surgery for mediastinal tumors using the da vinci SP system: Initial experience
Authors: Yang Bo , Chen Ruiji , Lin Yingxue , Liu Yang
Published: October 2022

Purpose

Studies of single-port robot-assisted thoracic surgery (RATS) using the da Vinci SP system, which uses a smaller surgical incision than the conventional multiport robot, have yet to be reported because of its smaller operating range. We report our initial experience using the da Vinci SP system in thoracic surgery for the resection of mediastinal tumors that requires a smaller workspace.

Description

Two patients diagnosed with superior mediastinal tumors underwent RATS performed with the da Vinci SP surgical system in January 2022. We used three-dimensional reconstruction to preoperatively determine the surgical incision. This is the first report of single-port RATS using the SP system in China.

Evaluation

R0 resection was achieved in both operations without complications. Operation times and bleeding volumes were similar to the use of multiport RATS. No perioperative complications occurred.

Conclusions

The da Vinci SP system can be used for the resection of superior mediastinal tumors. Case selection and preoperative planning should be performed prior to these surgeries.
The treatment efficacy of cortical bone trajectory (CBT) pedicle screws for lumbar degenerative disease in the Chinese Han population
Authors: Wu Jinhui , Lin Tao , Jiang Heng , Ma Jun , Zhang Ke , Zhao Jianquan , Zhou Xuhui , Wang Ce
Published: October 2022

Purpose

To provide reference data on CBT pedicle screws from CT measurements of L1 to L5 in the Chinese Han population and to assess the treatment efficacy of CBT pedicle screws in cases of lumbar degenerative disease.

Methods

In total, 100 patients were identified from the CT database for CBT morphometric measurement of the lumbar spine. According to sex and age, patients were divided into four groups. The diameter, length, and angle of the vertebral pedicle and trajectory were measured. Then, a total of 36 patients with lumbar degenerative disease were included in this study for clinical and radiographic evaluation. Demographic characteristics, health-related quality of life (HRQOL), and extent of intervertebral disc herniation and spondylolisthesis were evaluated.

Results

The mean diameter and the mean length varied from L1 to L5 in Groups I to IV. The lateral angles ranging from L1 to L5 were 8.9 to 9.2°, 8.7 to 12.2°, 8.7 to 11.2°, and 9.2 to 10.1° in Groups I to IV, respectively. The cephalad angles from L1 to L5 were 23.5 to 28.6°, 24.7 to 26.6°, 25.0 to 28.2°, and 24.7 to 27.9° in Groups I to IV, respectively. In the case series, all patients’ neurological function and HRQOL were significantly improved at the final follow-up (p < 0.0001), and 75% of patients achieved satisfaction.

Conclusions

The morphology of the lumbar vertebral pedicle varied from L1 to L5, and the trajectory was feasible and safe. CBT pedicle screws were effective in treating lumbar degenerative diseases and benefited the patients.
Effects of Allium mongolicum regel essential oil supplementation on growth performance, nutrient digestibility, rumen fermentation, and bacterial communities in sheep
Authors: Yaxing Zhao , Erdene Khas , Zhibi Bao , Changjin Ao , Chen Bai
Published: October 2022

The objectives of this research were to investigate the effects of Allium mongolicum Regel essential oil on growth performance, nutrient digestibility, rumen fermentation, and bacterial communities in sheep Twenty sheep were randomly divided into two dietary groups with 10 replicates each: (1) a basal diet without AMO as the control group (n = 10) and (2) a basal diet supplemented with 40 mg/kg AMO as the AMO group (n = 10). The average daily gain (ADG) was increased (P < 0.05), and the feed conversion ratio (FCR) was reduced (P < 0.05) in the AMO group compared with the control. The ruminal acetate, propionate, total volatile fatty acids (TVFA), and microbial protein (MCP) were higher (P < 0.05) in the AMO group than in the control. Moreover, ruminal pH and ammonia nitrogen (NH3-N) were lower (P < 0.05) in the AMO group than in the control. The relative abundances of the phylum levels of Firmicutes, Actinobacteriota, and Verrucomicrobiota were higher (P < 0.05) in the AMO group than in the control, and the relative abundances of Bacteroidetes and Spirochaetota were lower (P < 0.05) in the AMO group than in the control. The relative abundance of Prevotella and Prevotellaceae_UCG-003 at the genus level was increased (P < 0.05) in the AMO group compared with the control; however, the relative abundance of Succiniclasticum, Norank_f__F082, Christensenellaceae_R-7_group, and Norank_f__Muribaculaceae was decreased (P < 0.05) in the AMO group compared with the control. The activities of cellulase, α-amylase, and proteinase were higher (P < 0.05) in the AMO group than in the control. The apparent digestibility of dry matter (DM) and crude protein (CP) was increased (P < 0.05) in the AMO group compared with the control. In conclusion, AMO supplementation has the potential to improve growth performance. Moreover, supplementation with AMO improved nutrient digestibility, rumen fermentation, and bacterial communities in the rumen of sheep.