bluuebunny/crossref_metadata_2025 huggingface dataset:
A bibliometric evaluation of the publications on METTL3 mediated m6A from 2000 to 2022
Authors: Wang Yingji , Tan Xiaohua , Li Xuan’an
Published: April 2023

Background:Many studies have demonstrated the role of METTL3 and other proteins in the N6-adenylate methylation(m6A) of RNA. It plays a role in many diseases such as cancer and tumor. However, there is a lack of comprehensive and objective reports on m6A. Therefore, this study aims to visualize the publications to quantify and determine the current status and trends of m6A with METTL3 as the catalytic core in the past two decades.
Methods: Relevant studies published between 1 January 2000 and 24 November 2022 in English were retrieved from the Web of Science Core Collection. We used four scientometric tools (Citespace v6.1.R2, VOSviewer, Scimago Graphica and Microsoft Excel 2019) to quantify and identify the individual impact and cooperation information by analyzing annual publications, the collaboration of authors, co-citation of authors, source, journals and references, cluster and co-occurrence of keywords.
Results:A total of 1349 publications were retrieved and showed a rapid upward trend. Among the top ten journals and the top ten co-cited journals, there are many top journals and their sub journals such as NATURE, CELL, SCIENCE, which indicates the fruitful results in this field. China has the highest publications, while the United States has the most significant academic influence. Chinese researchers have a keen interest in this research field, and high-yield institutions, authors and publications account for most of the ranking. It can be seen from co-cited references and reference burst that the research on biomarker, diagnosis, RRID, YTHDC1, hepatitis B virus, osteogenic differentiation and other aspects has sprung up and continues. The research of m6A pays more attention to its significance in different diseases, such as inflammation and cancer, in addition to its mechanism and role in cells.
Conclusion:METTL3 mediated m6A has achieved fruitful results at the cellular and molecular levels. The research in this field is moving from the exploration of molecular mechanism to the refinement of inflammation, cancer and other diseases, and researchers also pay special attention to its clinical application value. As a biomarker or target, METTL3 is likely to play a role in disease early warning, prognosis prediction and treatment goals.
Nitro-functionalized Fe-MOFs for lithium-sulfur batteries
Authors: Ruan Yanli , Cai Haoyu , Feng Jinshuai , Lei Haikuo , Zheng Haitao
Published: April 2023

Energy storage by means of lithium-sulfur batteries holds great promise. They are inexpensive and have a high potential energy density. Unfortunately, the battery's cycling performance is greatly diminished by the shuttle effect of polysulfide. Metal-organic frameworks (MOFs) with high specific surface area, nanopore size, and plentiful porosity have been proven to help prevent polysulfide migration in recent years. In this research, partially nitro-functionalized MIL-101(Fe) has been produced by combining different proportion ligands. As an electron-withdrawing group, the nitro group can reduce the charge density of the metal sites and improve the adsorption capacity of the material to polysulfides. MIL-101-NO2-0.25 showed the performance with an initial discharge capacity of 1051.5 mAh g-1at a current density of 0.5 C and maintained at 908 mAh g-1after 250 cycles.
Appropriate indications for laparoscopic repeat hepatectomy
Authors: Masuda Takashi , Endo Yuichi , Amano Shota , Kawamura Masahiro , Fujinaga Atsuro , Kawasaki Takahide , Kawano Yoko , Hirashita Teijiro , Ohta Masayuki , Inomata Masafumi
Published: April 2023

Background: Laparoscopic repeat hepatectomy (LRH) has increased, but appropriate indications for LRH are unclear. This study aimed to clarify appropriate indications for LRH.
Methods: We retrospectively compared surgical outcomes between open RH (ORH) (n=57) and LRH (n=40) groups. To detect difficult cases of complete pure LRH, we examined patients with unplanned intraoperative hand-assisted laparoscopic surgery (HALS)/open conversion (n=6).
Results: In the LRH versus ORH group, as previous hepatectomy, laparoscopic (75% vs. 12%, p<0.001) and Hr0 (73% vs. 37%, p=0.002) were more frequently performed, and as RH procedure, Hr0 (88% vs. 47%, p=0.0002)was more frequently performed. S1 tumor cases were higher in ORH group (11% vs. 0%), but S2-6 cases were higher in LRH group(73% vs. 49%) (p=0.02). In LRH group, compared to the pure LRH patients, HALS/open conversion patients underwent significantly more previous hepatectomy with Hr2-3 (33% vs. 2.9%, p=0.033) and more RH procedures with HrS (33% vs. 2.9%, p=0.03). All LRH requiring a second hepatic hilar approach were HALS conversions.
Conclusion: Appropriate indications for LRH were previous hepatectomy was laparoscopic Hr0, and RH procedure was Hr0 for S2-6 tumor location. When RH is more than HrS requiring a second hepatic hilar approach, LRH should not be selected due to the high possibility of HALS/open conversion.
Un-differential processing of COSMIC-2 radio occultation measurements with 1-Hz LEO clock corrections
Authors: Guo Sheng , Zhang Shaocheng , Meng Xiangguang , Wu Yunlong , Lou Yidong , Lin Jian
Published: April 2023

Precise isolating atmospheric excess phase (AEP) is the fundamental work of Global Navigation Satellite System (GNSS) radio occultation technique towards reconstructing on the refractivity and other meteorological profiles. Currently, the COSMIC-2 satellites AEP products from University Corporation for Atmospheric Research (UCAR) were calculated by conventional single-differential approach at cost of complicating the processing and also amplified the excess phase noise. This study provided a strengthened un-differential method for COSMIC-2 AEP calculation such that the interpretation of raw occulting phase data could be enriched. The vital bias, receiver clock offset, was modeled and calibrated at 1s sampling via pre-processing of LEO precise orbit determination, which took both two antennas’ observations into estimator for intensifying orbit and clock precision. After combining the un-differential method with 1s LEO receiver clock corrections, COSMIC-2 AEP was calculated and collected to retrieve the refractivity profiles for statistical validation. Analyzation of 2579 RO profiles indicates that the mean bias of our results against COSMIC-2 official refractivity profiles were below 0.2% at height above 10km. Furthermore, external reference of European Centre for Medium-Range Weather Forecasts (ECMWF) analysis data was adopted for revealing the characteristics of bias and investigating the advantage of un-differential approach. Validation indicates that the standard deviation (STD) of un-difference derived refractivity against co-located referred profiles were around 1.17% and 1.28% for GPS and GLONASS occultations at altitude below 35km, appearing bias less than 0.04% against single difference method derived. The un-difference processing also shows higher accuracy than single difference in the lower troposphere. Hence, the proposed UD processing for COSMIC-2 radio occultation retrieval is feasible when the 1s LEO clock offsets were estimated for correction.
Robotic Surgery Perception and Attitude Among Medical Students in the Gulf Countries: A Multinational Cross-Sectional Study
Authors: Mousa Ahmed Hafez , Nukaly Houriah Yasir , Abujamai Jakleen Ziyad , Mohamed Rawan Elwalid Ali , AlShehabi Nagam , Duvuru Ruthwik , Ennab Farah , Nawaz Faisal A , Ahmed Ruqayyah Ali , Mosalli Rafat , Alshanberi Asim Muhammed , Ali Ehab A. Abo
Published: April 2023

Background:Robotic surgery comprises surgical procedures performed via robotic assistance. Recently, 75% more surgical robotic systems were installed in the United States’ hospitals. Saudi Arabia was the first regionally to introduce robotic surgery in 2003. Our study aims to compare and assess medical students’ knowledge and attitude on robotic surgery in the Gulf Region.Methods:This descriptive cross-sectional study assessed medical students across Kingdom of Saudi Arabia (KSA), United Arab Emirates (UAE) and Qatar. Our questionnaire evaluated demographical background, knowledge on robotic surgery, and lastly attitude towards robotic surgery.Results:The 278 participants (UAE: 144, KSA: 99, and Qatar: 35) were mostly female (66.9%), preclinical students (56.1%) and local (53.2%). Most UAE participants (86.8%) were aware of local robotic surgery applications, while most KSA (62.6%) and Qatar (82.9%) participants were not sure. Most KSA (60.6%), UAE (89.6%) and Qatar (68.6%) students correctly defined robotic surgery. Most participants from KSA felt neutral (36.3%) or agreed (33.3%) to use robotic surgery in clinical practice, while majority of UAE participants (57.6%) strongly agreed, and Qatar participants (51.4%) agreed. In addition, KSA respondents were more neutral (42.4%) on investing into robotic surgeries than UAE and Qatar participants.Conclusion:Most UAE participants were aware of local robotic surgery applications, while most KSA and Qatar participants were not sure. Mostmedical students in the Middle East had high level of knowledge on robotic surgery and its characteristics. However, attitudes on applicability of robotic surgery varied between positive and neutral outlooks depending on the country.
Green Cloud Computing Adoption Challenges and Practices: A Client's Perspective based Empirical Investigations
Authors: Ahmad Ashfaq , Khan Siffat Ullah , Khan Rafiq Ahmad , Alwageed Hathal Salamah , Al-Atawi Abdullah A.
Published: April 2023

Context
Over the last decade, the widespread adoption of cloud computing has spawned a new branch of the computing industry, known as green cloud computing. Cloud computing is improving, and data centers are increasing at regular frequencies to meet the demands of users. Cloud providers, on the other hand, pose major environmental risks because massive data centers use a large amount of energy and leave a carbon footprint. One possible solution to this issue is the use of green cloud computing. However, clients face significant difficulties in adopting green cloud computing.
Objective
This study aims to understand the problems faced by client organizations while considering green cloud computing. In addition, this study aims to empirically identify the solution to the challenges faced by green cloud computing practitioners.
Method
A questionnaire survey approach was used to get insight into green cloud computing practitioners concerning the challenges they faced and their solutions.
Results
Data were obtained from sixty-nine professionals in green cloud computing. The results revealed that "lack of quality of service", "lack of dynamic response", and "lack of services to satisfy client's requirements" are critical for green cloud computing. In addition, sixty-three practices for addressing the challenges in green cloud computing are also identified.
Conclusion
The identified challenges and practices of green cloud computing will benefit the client organizations to update and revise their process to consider green cloud computing. In addition, it will also assist vendor organizations in developing, planning, and managing systems concerning client satisfaction.
Verification of the existence of copper-based antifouling paint particles in the sea and their implications for marine microplastic degradation
Authors: NAKATANI Hisayuki , YAMASHIRO Kaito , UCHIYAMA Taishi , MOTOKUCHO Suguru , DAO Anh Thi Ngoc , KIM Hee-Jin , YAGI Mitsuharu , KYOZUKA Yusaku
Published: April 2023

Marine microplastics (MP) and microdebris were retrieved from the five sampling stations between Nagasaki port and Goto Island and were classified into six types. Three of these types, MP (A), Si-based (B), and Cu-based (C) paint particles, were predominant. Type C had no depth dependence, regardless of the presence of cuprous oxide with high specific gravity. The dominant size of type C was less than 10 µm and contained 30 to 50 mol% of Cu content. The long stay behavior of type C suggested that it had implications for degrading type A. To clarify this, polypropylene (PP) film samples containing cuprous oxide were prepared, and their accelerated degradation behavior in seawater was investigated using the advanced oxidation process method using the sulfuric acid radical initiator in seawater of different salinity concentrations. Infrared spectroscopy revealed the formation of a copper soap compound in seawater. Scanning electron microscopy/energy-dispersive X-ray spectroscopy analysis indicated that the chemical reactions between Cl− and cuprous oxide produced Cu+ ions. Changes in the number of polymer chain scissions were investigated at various salinity concentrations of seawater. These findings confirm that Cu+ species promote PP autoxidation.
Cuprotosis Clusters Predicts Prognosis and Immunotherapy Response in Low-grade glioma
Authors: Zhu Wenjun , Fu Min , Li Qianxia , Chen Xin , Li Xiaoyu , Luo Na , Tang Wenhua , Yang Feng , Chen Ziqi , Zhang Yiling , Zhang Yuanyuan , Peng Xiaohong , Hu Guangyuan
Published: April 2023

Cuprotosis, a new cell death mode, has recently received increasing attention. However, the role of cuprotosis in low-grade glioma (LGG) remains unclear. In this study, we explored the relationship of cuprotosis with LGG patient prognosis and immune status. We divided LGG patients into cuprotosis clusters A and B based on the expression of 18 cuprotosis genes in LGG patients. The prognosis of patients in cuprotosis cluster A was better than cuprotosis cluster B. Cuprotosis clusters showed different immune cell infiltration and biological functions. We screened the differentially expressed genes (DEGs) between the different cuprotosis clusters and identified 6 DEGs (TNFRSF11B, METTL7B, SSTR2, OXTR, CDKN2C, and H19) to construct a cuprotosis-related prognostic signature to predict the prognosis of LGG patients through univariate cox-lasso-multivariate cox regression analysis. The signature split LGG patients into two risk groups. High-risk group showed significantly shorter overall survival (OS) time than low-risk group in the training group, internal and external validation groups. The nomogram further precisely predicted patients' 1, 3 and 5-year OS. The Sankey diagram visually displayed the correspondence of different clusters with risk scores and survival status. Similarly, we also found the relationship of tumor microenvironment (TME) infiltration and tumor mutation burden (TMB) with risk scores, and the high-risk group might benefit more from immunotherapy. In conclusion, our study highlighted the significant role of cuprotosis in LGG prognosis and TME infiltration. The cuprotosis-related prognostic signature would help us predict the prognosis of LGG patients and understand the LGG immune infiltration characterization, providing potential molecules for further molecularly targeted therapies and immunotherapeutic interventions in LGG.
Serum thiamine levels was relative factors in the incidence of readmission due to heart failure
Authors: Watanabe Shingo
Published: April 2023

Background and Aims
Previous studies have reported that 5-91% of patients with heart failure have thiamine deficiency. With the aging of heart failure patients, the number of heart failure patients with thiamine deficiency may increase. It is important to know the prevalence and clinical characteristics of heart failure patients with thiamine deficiency.
The purpose of our study is to investigate the prevalence and clinical characteristics of thiamine deficiency in patients with heart failure.
Methods and Results
The subjects were 256 cases who were admitted to our hospital for acute heart failure. All patients were classified into two groups, a thiamine deficient group (TD group N=72) and a normal thiamine group (NT group N=184), with the serum vitamin 24 ng / ml, which is the standard value in Japan, as the cutoff value. The clinical features and outcomes of both groups were investigated. The endpoint was set to one-year of cardiac death and readmission due to heart failure.
Of all 256 cases, 72 (28.1%) had thiamine deficiency.
The TD group had a significantly higher incidence of one-year cardiac death (25.0% VS 13.0% P=0.02) and readmission due to heart failure (38.9% VS 23.9% P=0.01)
Multivariate logistic regression analysis revealed that serum thiamine levels was relative factors in the incidence of readmission due to heart failure.
Conclusion
Thiamine deficiency is not uncommon in patients with heart failure.
Patients with heart failure who have thiamine deficiency have a high incidence of readmission due to heart failure, so treatment aimed at preventing readmission is necessary.
Distribution of normalized pulmonary transit time per pathology in a population of routine CMR examinations
Authors: Jossart Adrien , Gerber Bernhard , Houard Laura , Pilet Bernard , O'Connor Sean , Gilles Raymond
Published: April 2023

Introduction: Pulmonary Transit Time (PTT), defined as the time required for a contrast bolus to pass from the right ventricle to the left ventricle, is a surrogate for noninvasive preload assessment. It is used in different imaging modalities: pulmonary angiography, echocardiography and cardiac magnetic resonance (CMR).
Purpose: Many recent studies have highlighted the prognostic value of PTT. Therefore, we sought to evaluate PTT in a consecutive cohort of patients undergoing CMR.
Methods: We retrospectively evaluated PTT normalized for heart rate in 278 patients (66% male, mean age 58±11 years) who underwent CMR between August 2017 and November 2021 with a diagnosis of dilated cardiomyopathy, infarct, hypertrophy, valvular, myocarditis, other pathology or no pathology (“normal”).
Results: Normalized pulmonary transit time (nPTT) was higher in men than in women (8.4 ± 1.3 beats vs 7.5 ± 1.1 beats, p = 0.002) in the “normal” group. nPTT was moderately correlated with left ventricular end-diastolic volume (LVEDV) (r² = 0.19 ; p < 0.001), left ventricular end-systolic volume (LVESV) (r² = 0.34 ; p < 0.001) and left ventricular ejection fraction (LVEF) (r² = 0.29 ; p < 0.001). nPTT was significantly higher in patients suffering from dilated cardiomyopathy (11.3 ± 5.4 beats ; p < 0.001), infarct (9.5 ± 2.9 beats ; p < 0.001) or valvular disease (9.5 ± 3.1 beats ; p = 0.006) than in patients included in the “normal” group (7.9 ± 1.3 beats).
Conclusion: nPTT is a novel major surrogate for the preload status of the patient. Its value depends on gender and type of pathology.
Optimal bounds for complete elliptic integral from the quasi-arithmetic mean and Seiffert-like mean
Authors: Shi Yong-Guo , Li Na
Published: April 2023

We give the new double inequalities for the quasi-arithmetic mean and Seiffert-like mean. As applications, two new optimal bounds are presented for the complete elliptic integral of the second kind. And numerical comparisons illustrate that some bounds of our results are better than before.
AMS(2020) subject Classification: 26E60, 33C75, 26D20.
Monitoring Mangrove Forest Degradation in the Sunderban Region of India using Remote Sensing Techniques: Implications for CO 2 Absorption and Surface Energy Balance from 2000-2020
Authors: Maske Ambadas Bandu
Published: April 2023

The study uses novel remote sensing approach for understanding the changes in mangrove forest of Sunderban region of India. The study tried to estimate the changes in carbon emissions using GIS and remote sensing. The study quantifies the changes in different heat fluxes of surface energy balance i.e., net radiations, ground heat flux, latent heat flux. Unavailability of real time atmospheric profile data is the major limitation of study. study presents an analysis of the changes in carbon emissions, energy fluxes, and land-use and land-cover (LULC) in the Sunderban forest from 2000 to 2020. The results show a significant loss of mangroves, which has led to a decline in carbon stocks and an increase in CO2 emissions. Changes in the energy fluxes, particularly the decrease in latent heat flux, were found to be associated with the reduction in the area under mangrove. The LULC analysis reveals changes in the surface temperature due to the reduction in vegetation cover, which has implications for the local and regional climate. The findings underscore the urgent need for conservation and restoration efforts to protect the vital ecosystem services provided by mangrove forests and mitigate the adverse effects of climate change.
The significance of calibration to the quantum-mechanical description of physical reality
Authors: Krechmer Ken
Published: April 2023

This paper is a response to the EPR paper titled: "Can quantum-mechanical description of physical reality be considered complete?", published in Physical Review in 1935. A quantum-mechanical (QM) measure function describes a distribution of local results, while each experimental measurement process produces one result as exact as allowed by a measuring apparatus calibrated to a non-local unit standard. Repeating experimental measurements produces a Gaussian distribution of measurement results. These QM and experimental measurement result distributions can be compared. To compare a QM measure function describing a distribution of eigenvectors to a distribution of repetitive experimental measurement results, it is necessary to determine, by calibration, the precision of the eigenvectors to the same standard as the experimental results, because each eigenvector evidences uncertainty relative to a standard. When the calibration process is recognized as formal as well as empirical, QM measure function and experimental measurement process results are unified.
Automatic Test Data Generation based on the Prime Path Coverage Criterion: A Grouping-based GA Approach
Authors: Damia Amirhossein , Esnaashari Mehdi , Parvizimosaed Mohammadreza
Published: April 2023

Software testing is the process of running an application with the goal of finding bugs and subsequently improving its quality. Software testing, as a key process, plays a role in ensuring the quality of software systems. Testing is currently considered an industry in the field of software. Given that about 40% of the cost of producing any software is spent on testing, creating tools for automatically generating test data will significantly reduce the current costs of software development. This process can be considered an optimization problem, and thus, search algorithms can be used for tackling it. The Genetic Algorithm (GA) is one of the widest algorithms in this field. In this paper, we have proposed a novel GA approach, called Group-based GA (G-GA), which differs from the standard GA algorithm in the following ways. First of all, a new fitness function has been utilized that uses search space information to guide the population. The population is divided into four groups, each of which is updated according to its fitness level. Finally, in the proposed algorithm, the selection operator has been omitted and thus, the algorithm has less complexity and calculations than the standard GA. Also, the proposed algorithm considers a good level of exploration and exploitation at each step. Experiments have shown that the proposed G-GA method, in terms of the convergence speed and the search time, significantly outperforms the basic GA, its variations, PSO, Tabu Search, and Simulated Annealing.
Elucidation of the mechanisms of human cathelicidin-derived antimicrobial peptides (LL-37 and its truncated LL-31) against Burkholderia pseudomallei
Authors: Tun Wonn Shweyi Thet , Phophetleb Onanong , Klaynongsruang Sompong , Daduang Sakda , Taweechaisupapong Suwimol , Patramanon Rina
Published: April 2023

Melioidosis is an endemic disease caused by Burkholderia pseudomallei found in tropical areas. The bacterium is intrinsically resistant to conventional antibiotics, making treatment difficult. Cathelicidin-derived peptides LL-37, and its truncated LL-31 played a crucial role in innate immune defense against infected microorganisms. LL-31 showed superior killing activity than LL-37 in reducing bacteria viability, combination with antibiotic ceftazidime (CAZ), outer and inner membrane permeabilization, membrane depolarization, and affinity binding to lipopolysaccharide (LPS) of B. pseudomallei. Both peptides had similar content of alpha-helical structure. This study provided insights into the molecular mechanisms of LL-37 and its truncated form, LL-31. By utilizing the knowledge gained from this study, researchers may be able to improve treatment options and help combat the spread of antibiotic-resistant bacteria.
Isolation and characterization of two glycolipopeptids biosurfactants produced by a Lactobacillus plantarum strain isolated from green olive curing water
Authors: AMMAR Ameni Ben , BOUASSIDA Mouna , BOUALLEGUE Amir , FOURATI Nada , Gerardi Gisela , Muñiz Pilar , Benito Jose Manuel , GHRIBI Dhouha
Published: April 2023

Microbial surfactants are natural amphiphilic compounds with high surface activities and emulsifying properties. Due to their structural diversity, low toxicity, biodegradability, and chemical stability in different conditions, these molecules are potential substitutes for chemical surfactants; their interest has grown significantly over the last decade. The current study focuses on the isolation, identification, and characterization of a lactic acid bacteria that produces two forms of biosurfactants. The OL5 strain was isolated from green olive fermentation and identified using MALDI/TOF and ADNr16S amplification. Emulsification activity and surface tension measurements were used to estimate biosurfactant production. The two biosurfactants derived from L. plantarum OL5 presented good emulsification powers in the presence of various oils. They were also shown to have the potential to reduce water surface tension from 69 mN/m to 34 mN/m and 37 mN/m within a critical micelle concentration (CMC) of 7 mg/ml and 1.8 mg/ml, respectively, for cell bound and extracellular biosurfactants. Thin layer chromatography (TLC) and FT-IR were used to analyze the composition of the two biosurfactants produced. the obtained data revealed that the two biomolecules consist of a mixture of carbohydrates, lipids and proteins. We demonstrated that they are two anionic biosurfactants with glycolipopeptide nature which are stable in extreme conditions of temperature, pH and salinity.
LC/MS based untargeted lipidomics reveals lipid signatures of Non-Puerperal Mastitis
Authors: Chen Xiaoxiao , Shao Shijun , Wu Xueqing , Feng Jiamei , Qu Wenchao , Gao Qingqian , Sun Jiaye , Wan Hua
Published: April 2023

Background: Non-Puerperal Mastitis (NPM) is a type of disease that occurs during non-lactation accompanied by redness, swelling, heat and pain which is easily confused with breast cancer. The etiology of NPM is not yet clear. There is growing evidence from research showing that lipid metabolism may play an important role in NPM. This study aimed to use lipidomics to provide evidence for the etiology and potential therapeutic approaches for NPM.
Method: Liquid chromatography mass spectrometry (LC/MS)-based untargeted lipidomics analysis combined with multivariate statistics were performed on investigating the NPM lipid change in breast tissue.
Results: The results revealed significant differences in lipidomics profiles and a total of 16 subclasses with 14,012 different lipids were identified in positive and negative ion modes. Among these lipids, Triradylcglycerols (TAGs), Glycerophosphoethanolamines (PEs) and Glycerophosphoglycerophosphoglycerols (CLs)are top three lipid components between the NPM and control groups. Subsequently, 35 significantly different lipid metabolites were screened as potential biomarkers, such as 15(S)-Hydroxyeicosatrienoic acid, Lipoxin A4 (LXA4), Lipoxin B4 (LXB4) and LysoPC(16:0), and they showed good diagnostic performance. Detailed pathway analysis revealed that these dysregulated lipids are closely related to arachidonic acid metabolism, glycerophospholipid metabolism.
Conclusions: This study showed that NPM patients' breast tissue exhibited altered lipid groups. In addition, the lipid metabolites screened and the pathways identified in this study may be potential targets for future studies of the pathogenesis of NPM.
Trial registration: Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine (Number: 2019-702-57; Date: July 2019).
TRIM32 Reduced the Recruitment of Innate Immune Cells and the Killing Capacity of Listeria monocytogenes by Inhibiting Secretion of Chemokines
Authors: OuYang Xuan , Liu Peng , Zheng Yuling , Jiang Hua , Lv Qingyu , Huang Wenhua , Hao Huaijie , Pian Yaya , Kong Decong , Jiang Yongqiang
Published: April 2023

Listeria monocytogenes (Lm) is a facultative, intracellular Gram-positive pathogenic bacterium that causes sepsis, a condition characterized by persistent excessive inflammation and organ dysfunction. However, the pathogenesis of Lm-induced sepsis is unknown. In this research, we discovered that TRIM32 is required for innate immune regulation during Lm infection. Trim32 deficiency remarkably reduced bacteremia and proinflammatory cytokine secretion in mice with severe Lm infection, preventing sepsis. Trim32-/- mice had a lower bacterial burden after Lm infection and survived significantly longer than wild-type (WT) mice, as well as lower serum levels of inflammatory cytokines TNF-α, IL-6, IL-18, IL-12p70, IFN-β, and IFN-γ at 1 day post infection (dpi) compared to WT mice. On the other hand, the chemokines CXCL1, CCL-2, CCL-7, and CCL-5 were increased at 3 dpi in Trim32-/- mice compared to WT mice, implying increased recruitment of neutrophils and macrophages to clear Lm. Furthermore, Trim32-/- mice also produced more macrophage-associated iNOS. Collectively, our findings suggest that TRIM32 reduces innate immune cells recruitment and Lm killing ability by inhibiting chemokines secretion.
Tunable random laser in capillary with Nile red solution and TiO2 nanoparticles
Authors: Fang Yuwei , Hu Jigang , Huang Chan
Published: April 2023

We report a tunable random laser in Nile red (NR) solution with TiO2 nanoparticles. The good lasing characteristics of NR and random scattering environment provided by TiO2 bring excellent random laser emission. We analyze the influence of TiO2 number density to the threshold of random laser and draw the output energy stability via pump time. Furthermore, considering the solvatochromism of NR, the solvent is substituted by ether and acetone while the optimal number densities of TiO2 in each system are obtained respectively. In addition, we use sulfuric acid to change the polarity of the sample and obtain the random laser spectra that change continuously with the polarity of the solution. This combination of NR and TiO2 could shed new light upon the generation and tuning of random laser.
Episode I. Exploring Streptococcus pneumoniae capsular typing through MALDI-TOF mass spectrometry and machine-learning algorithms in Argentina: Identifying prevalent NON PCV13 serotypes alongside PCV13 serotypes.
Authors: Zintgraff Jonathan , Rocca Florencia , Eluchans Nahuel Sánchez , Irazu Lucía , Moscoloni Maria Alicia , Lara Claudia , Santos Mauricio
Published: April 2023

Laboratory surveillance of Streptococcus pneumoniae serotypes is crucial for the successful implementation of vaccines to prevent invasive pneumococcal diseases. The reference method of serotyping is the Quellung reaction, which is labor-intensive and expensive. In the last few years, the introduction of MALDI-TOF MS into the microbiology laboratory has been revolutionary. In brief, this new technology compares protein profiles by generating spectra based on the m/z ratio. We evaluated the performance of MALDI-TOF MS for typing serotypes of S. pneumoniae isolates included in the PCV13 vaccine using a machine learning approach. We challenged our classification algorithms with a total of new 215 isolates of S. pneumoniae from Argentinian nationwide surveillance. Our work involved a thorough evaluation and comparison of multiple machine learning models. Through this evaluation, we achieved high accuracy values, indicating the effectiveness of the approach taken in solving the problem. Additionally, we attained high kappa index values, indicating that the predictions made by the models were highly accurate and reliable. We also carefully considered the strengths and limitations of each model. As such, our results are highly valuable and have important implications for future research and applications.
Determinants of Pregnancy Outcomes after Assisted Reproductive Therapy: A Sample from the West Bank, Palestine
Authors: Arafat Hasan , Qamhia Diaeddin , Maqboul Husam , Al-Khayyat Abdulsalam
Published: April 2023

Objective: Infertility is a public health issue with a huge impact on the well-being of affected couples. Despite the traditional valuing of fertility within the Palestinian society, no studies were conducted to detect the determinants of pregnancy outcome in Palestine. This paper aims at detecting the characteristics and pregnancy outcome after assisted reproductive technology (ART) of a sample of the Palestinian society.
Materials & Methods: A retrospective observational study was carried out at Razan Medical Center for Infertility. Subjects were assigned 2 categories based on the type of infertility, 7 different categories according to the cause of infertility, and 3 categories for the modality of treatment. Age at marriage, age at presentation, duration of infertility, in addition to regularity of menstruation were also studied. Biochemical pregnancy was considered the endpoint for the purpose of analysis.
Results: We reviewed the files of 459 subjects diagnosed with infertility. Of the studied subjects, 79.74% had primary infertility, while 20.26% had secondary. According to diagnosis, 28.85% were found to be infertile due to anovulation, 2.86% due to endometriosis, 16.74% attributed to male factor, 3.3% had tubal damage, while 34.80% had no explainable cause. 13.43% of cases were multifactorial. Four biochemical markers were assessed in our study, thyroid-stimulating hormone (TSH) (x̄=2.32±2.46), leutinizing hormone (LH) (x̄=6.71±4.90), follicle-stimulating hormone (FSH) (x̄=6.59±6.11), and human prolactin (hPRL) (x̄=41.88±6.50). The menstrual cycle was regular in 70.58% of subjects, in contrast to 29.41% with irregular cycles. Female mean age at presentation 22.76±5.58, while their mean age of marriage was 22.76±4. Mean duration of infertility was 3.97±6.87. Patients were treated via 3 modalities, ovarian stimulation (2.2%), intrauterine insemination (IUI) (29.58%), and intracytoplasmic sperm injection (ICSI) (68.21%), with a rate of success of 51.85%. Of the studied factors, only diagnosis, FSH, and modality of treatment had a significant impact on the outcome, with p-values of 0.040, 0.003, and <.0.001, respectively.
Conclusions: there is a strong relationship between diagnosis prior to intervention, level of FSH and treatment modality on successful outcome of ART. ICSI was found to be superior to other modalities of treatment. We recommend starting with ICSI to save patient time & effort.
Changes of Extreme Climate Indices on the Mongolian Plateau during 1981-2020 Based on ERA5 Reanalysis
Authors: Zhu Guoying , Zhao Xinyi
Published: April 2023

The Mongolian Plateau (MP) is susceptible to extreme climate events due to its unique geographical location and socio-economic conditions. The focus of this study was to analyze the changes in extreme climate indices on the MP based on the ERA5 reanalysis dataset, as well as to investigate the relationship between these indices and atmospheric circulation indices. 1) Results indicated that the inter-annual variation trends of Summer days, Warm days, and Warm nights have significantly increased across the entire MP, with the central region experiencing a higher probability of extreme hot events. The inter-decadal variation of extreme hot indices rose the fastest from period 2 (1991-2000) to period 3 (2001-2010). 2) Results showed a substantial decrease in extreme cold events throughout the study period, with the most rapid decrease observed from period 1 (1981-1990) to period 2 (1991-2000), except for Frost days. 3) There was a decrease in extreme wet indices from 1981-2020. The Simple Precipitation Intensity Index (SDII) and Total precipitation in wet days (PRCPTOT) exhibited a significant descending trend in the northern and northeastern MP. Decadal variations showed a decrease in extreme precipitation, with the most significant decline observed between period 2 (1991-2000) to period 3 (2001-2010). 4) Extreme precipitation indices indicate a negative correlation with the Atlantic Meridional Mode (AMM). The Arctic Oscillation (AO) displayed a negative correlation with extreme cold indices, whereas the Pacific Decadal Oscillation (PDO) demonstrated a negative correlation with extreme hot indices.
Nutrient Decline and Metal Pollution Since the Second Industrial Revolution: The Red Sea
Authors: Cai Chunzhi , Delgado-Huertas Antonio , Agusti Susana
Published: April 2023

Reconstructing element concentrations in sediment cores helps identify historical natural or human-induced environmental changes. This study investigates variations in 15 element concentrations in Red Sea (a hot spot for biodiversity) sediment cores over the past five centuries. The findings reveal a significant change in sediment element accumulation rates (EAR) following the Second Industrial Revolution (~1870) and the opening of the Suez Canal. In the North, where industrial activities become prevalent, we observe an increase in the mean EAR of Fe, Cd, V, Zn, Cu, and Cr from 4.56% to 17.6%, with positive slope change rates ranging from 332% (Fe) to 1003% (Cu). Conversely, in the South, we observe a decline in the mean EAR of Mg, total N, total organic C, and Ca from -8.5% to -17.8%, with negative slope change rates ranging from -83% (Ca) to -13980% (Mg). The results reveal the increasing accumulation of trace metals from human activities in recent decades in the North, whereas the South is experiencing a decline in nutrient input from the Indian Ocean associated with ocean warming. These two challenges may be synergistic and have a detrimental effect on the Red Sea ecosystems.
Network Pharmacology Approach for the Mechanism of Traditional Chinese Medicine in Acne
Authors: WANG Yunong , ·NIE Jing , ·WANG Ying , ·ZHANG Fengchuan
Published: April 2023

Objective To explore the molecular mechanism of traditional Chinese medicine (TCM) in the treatment of acne based on network pharmacology.
Methods The main active components of TCM were screened by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP）and Encyclopedia of Traditional Chinese Medicine (ETCM) database and the target was predicted by Swiss Target Prediction website. At the same time, the related targets of ance were screened from Gene Card database, Disgent database, and Therapeutic Target Database (TTD)database. The protein-protein interaction network was constructed using String website and Cytoscape 3.9.1 software after mapping the component target and disease target. Molecular docking technology was used to match potential active components with key targets. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were analyzed by Metascape website. Cytoscape3.9.1 software was used to construct the herb-component-target-pathway network.
Results 207 active ingredients and 1722 potential targets were screened from TCM, among which 70 core targets and 20 pathways were closely related to the treatment of acne. Molecular docking results showed that the active ingredients and their key targets had good binding activity.
Conclusion TCM has biological support in treating acne. Different proteins have synergistic effects, in which SRC, MAPK1, STAT3, RELA, RXRA, EP300, NFKB1, and NFKBIA, etc. play a key role. Biological processes are mainly involved in hormone synthesis, endocrine regulation, angiogenesis, cell signal transduction, inflammation, etc. These targets are mainly concentrated in multiple signaling pathways such as MAPK and Rap1 signal pathways, which play key roles in anti-inflammatory, anti-skin keratosis, regulation of cell adhesion, repair of the skin barrier, and so on.
The IMPACT Score: A new score to predict the risk of early mortality in cardiogenic shock patients treated with venoarterial extracorporeal membrane oxygenation
Authors: Simon VEYRET , Charles VIDAL , Leandre GIRARD , Laurence DANGERS , Baptiste EMOND , Berenice PUECH , Jerome ALLYN , Nicolas ALLOU , Eric BRAUNBERGER
Published: April 2023

Purpose :
Venoarterial-ECMO (VA-ECMO) is indicated in the management of refractory cardiogenic shock. There is currently no risk factor for early mortality, i.e. occurring within 72 hours of implantation. Highlighting them could avoid unnecessary recourse to this costly and heavy-care technique. The objective of this study is to determine the risk factors associated with early mortality in patients implanted with VA-ECMO and to construct a prognostic score.
Methods:
This single-center retrospective study included all patients treated with VA-ECMO at Felix Guyon Hospital in Reunion Island over the period 2014-2020. The pre-implantation risk factors for early mortality were sought by logistic regression and integrated into the IMPACT score.
Results:
A total of 165 patients were included in the study. Early mortality (<72 hours) was 20% (33 patients). Four pre-implantation risk factors were identified and integrated into the IMPACT score: lactate levels > 8mmol/L (Odds ratio (OR) = 4.2; p = 0.007), platelet count < 100G/L (OR = 4.9; p = 0.022), renal replacement therapy (OR = 7.1; p = 0.001), and cardiac arrest (OR = 3.2; p = 0.036). The IMPACT score had an AUC of 0.80 [95% Confidence Interval 0.73-0.88].
Conclusion:
The IMPACT score can help the physicians to identify patients who do not expect any benefit from VA-ECMO. Its validity should be tested in other populations treated with VA-ECMO.
Modeling hydrodynamic regime of the technogenic aquifer within the Noyabrsky site of the Aikhal mine
Authors: Yannikov A. M. , Struchkova A. S. , Korepanov A. Yu.
Published: April 2023

The Noyabrsky site is used for the injection of highly mineralized drainage waters formed during the development of the Aikhal kimberlite pipe (Republic of Sakha (Yakutia), Russia). The water injected into the wells of the site since 2013 has formed a localized intrapermafrost technogenic aquifer. Taking into account the porosity parameters of the formed permafrost reservoirs, as well as the confirmed useful capacity of the site, its operation is planned to continue until 2044 at the very least. Despite relatively small injection volumes (up to 430 m3/day), forecasting dynamical changes in the hydrodynamic regime of the technogenic water horizon is a challenging task, which is of significant practical importance for the provision of environmental and industrial safety. The assessment of the anthropogenic impact, as well as the subsequent forecasting of the hydrodynamic regime dynamics within the Noyabrsky site, were carried out by modeling methods in the Modflow software. The brine lens formed today is characterized by uneven porosity parameters, which is due to the peculiarities of heat and mass transfer when using permafrost for drainage water injection. Based on the results of predictive modeling a conclusion can be derived that the use of the method of Aikhal mine drainage water injection into the permafrost strata at the Noyabrsky site allows to reduce the influence degree of mining and production operations on the geological environment of the studied area through its localization on an intentionally limited, relatively small area, where injection is carried out, as well as on the environment, by preventing drainage brines from entering surface waters until 2044.
Studying Baikal region sand properties
Authors: Yakovleva A. A. , Guseva E. A. , Konstantinova M. V.
Published: April 2023

The study is aimed at a more detailed determination of the thermal properties of Baikal region sands and establishment of their dependence on geological and mineralogical characteristics in order to assess the possibility of using this raw material in the food industry. The objects of the study are sands sampled on the southwestern coast of Lake Baikal. The subject of the study is the mineralogical composition of sand, its grain size composition and heat capacity. The mineral composition of sand samples and their crystal structure were studied using the method of X-ray phase analysis. A standard sieve analysis method was used for granulometric estimation of sands. The average heat capacity of sand samples was determined by the mixing method. Based on the analysis of the obtained X-ray diffraction patterns, the mineralogical composition of the studied samples was determined. It was found that quartz predominates in their composition since its percentage in samples amounts to 40–60 %. The proportion of such minerals as albite and anorthoclase is 15–30 % in different samples. Less than 5 % in each sample accounts for dickite and indialite. The presence of the mineral of anthophyllite has been found only in one sample in the amount of about 9 %. The results of granulometric composition determination show that a fraction, which accounts for 70–90 %, can be isolated in most samples. The sample from the town of Slyudyanka was the only one where the fractions of different sizes were present in approximately equal amounts. For this sample, the dependence of heat capacity on the grain size was determined. In other samples, the difference in certain heat capacity values correlated well with their mineralogical composition. The results of the conducted studies and their analysis  allow to conclude that Slyudyanka and Baikalsk sands are the most attractive sands in terms of high heat capacity. The sand with the grain size from 0.125 to 0.25 mm is found to be the most suitable for the use as a thermal storage medium. This fraction is predominant in the sand of the town of Baikalsk.