bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative genome analysis of the genus Shewanella unravels the association of key genetic traits with known and potential pathogenic lineages
Authors: Cerbino Gabriela N. , Traglia German M. , Ayala Nuñez Teolincacihuatl , Parmeciano Di Noto Gisela , Ramírez María Soledad , Centrón Daniela , Iriarte Andrés , Quiroga Cecilia
Published: February 2023

Shewanella spp. are Gram-negative rods widely disseminated in aquatic niches that can also be found in human-associated environments. In recent years, reports of infections caused by these bacteria have increased significantly. Mobilome and resistome analysis of a few species showed that they are versatile; however, comprehensive comparative studies in the genus are lacking. Here, we analyzed the genetic traits of 144 genomes from Shewanella spp. isolates focusing on the mobilome, resistome, and virulome to establish their evolutionary relationship and detect unique features based on their genome content and habitat. Shewanella spp. showed a great diversity of mobile genetic elements (MGEs), most of them associated with monophyletic lineages of clinical isolates. Furthermore, 79/144 genomes encoded at least one antimicrobial resistant gene with their highest occurrence in clinical-related lineages. CRISPR-Cas systems, which confer immunity against MGEs, were found in 41 genomes being I-E and I-F the more frequent ones. Virulome analysis showed that all Shewanella spp. encoded different virulence genes (motility, quorum sensing, biofilm, adherence, etc.) that may confer adaptive advantages for survival against hosts. Our data revealed that key accessory genes are frequently found in two major clinical-related groups, which encompass the opportunistic pathogens Shewanella algae and Shewanella xiamenensis together with several other species. This work highlights the evolutionary nature of Shewanella spp. genomes, capable of acquiring different key genetic traits that contribute to their adaptation to different niches and facilitate the emergence of more resistant and virulent isolates that impact directly on human and animal health.
Identification and validation of a risk model and molecular subtypes based on tryptophan metabolism-related genes to predict the clinical prognosis and tumor immune microenvironment in lower-grade glioma
Authors: Li Wenxia , Ling Ling , Xiang Lei , Ding Peng , Yue Wei
Published: February 2023

Background

Lower-grade glioma (LGG) is one of the most common malignant tumors in the central nervous system (CNS). Accumulating evidence have demonstrated that tryptophan metabolism is significant in tumor. Therefore, this study aims to comprehensively clarify the relationship between tryptophan metabolism-related genes (TRGs) and LGGs.

Methods

The expression level of TRGs in LGG and normal tissues was first analyzed. Next, the key TRGs with prognostic value and differential expression in LGGs were identified using the least absolute shrinkage and selection operator (LASSO) regression analysis. Subsequently, a risk model was constructed and Consensus clustering analysis was conducted based on the expression level of key TRGs. Then, the prognostic value, clinicopathological factors, and tumor immune microenvironment (TIME) characteristics between different risk groups and molecular subtypes were analyzed. Finally, the expression, prognosis, and TIME of each key TRGs were analyzed separately in LGG patients.

Results

A total of 510 patients with LGG from The Cancer Genome Atlas (TCGA) dataset and 1,152 normal tissues from the Genotype-Tissue Expression (GTEx) dataset were included to evaluate the expression level of TRGs. After LASSO regression analysis, we identified six key TRGs and constructed a TRGs risk model. The survival analysis revealed that the risk model was the independent predictor in LGG patients. And the nomogram containing risk scores and independent clinicopathological factors could accurately predict the prognosis of LGG patients. In addition, the results of the Consensus cluster analysis based on the expression of the six TRGs showed that it could classify the LGG patients into two distinct clusters, with significant differences in prognosis, clinicopathological factors and TIME between these two clusters. Finally, we validated the expression, prognosis and immune infiltration of six key TRGs in patients with LGG.

Conclusion

This study demonstrated that tryptophan metabolism plays an important role in the progression of LGG. In addition, the risk model and the molecular subtypes we constructed not only could be used as an indicator to predict the prognosis of LGG patients but also were closely related to the clinicopathological factors and TIME of LGG patients. Overall, our study provides theoretical support for the ultimate realization of precision treatment for patients with LGG.
Total sleep deprivation selectively impairs motor preparation sub-stages in visual search task: Evidence from lateralized readiness potentials
Authors: Song Tao , Du Fangchong , Xu Lin , Peng Ziyi , Wang Letong , Dai Cimin , Xu Mengmeng , Zhang Ying , Shao Yongcong , Weng Xiechuan , Li Shijun
Published: February 2023

Introduction

Many studies have provided evidence of a damage effect triggered by total sleep deprivation (TSD). However, it remains unclear whether the motor preparation processing is affected by TSD.

Methods

In the current study, 23 volunteers performed a stimulus-response compatibility visual search task before and after TSD while undergoing spontaneous electroencephalography (EEG).

Results

Repeated-measures analysis of variance revealed that: Compared with that at baseline, the visual search task’s accuracy decreased after TSD, while the response time variance increased significantly. The peak amplitude of the stimulus-locked lateralized readiness potential (LRP) induced by a compatible stimulus was significantly more negative than that induced by an incompatible stimulus before TSD, whereas this difference was not significant after TSD. However, when taking sleep status into consideration, there were no significant main or interaction effects on response-locked LRPs.

Discussion

Our findings suggest that TSD damages visual search behavior, selectively impairs the earlier sub-stages of motor preparation (sensory integration). These findings will provide a new perspective for understanding the effects of sleep loss.
Intermittent fasting and immunomodulatory effects: A systematic review
Authors: He Zhangyuting , Xu Haifeng , Li Changcan , Yang Huayu , Mao Yilei
Published: February 2023

Introduction

strategy of periodic food restriction and fixed eating windows, could beneficially modify individuals by losing body weight, regulating glucose or lipid metabolism, reducing blood pressure, and modulating the immune system. Specific effects of IF and its mechanisms have not yet been assessed collectively. Thus, this systematic review aims to summarize and compare clinical trials that explored the immunomodulatory effects of IF.

Methods

After screening, 28 studies were included in this systematic review.

Results

In addition to weight loss, IF could benefit health subjects by strengthening their circadian rhythms, migrating immune cells, lower inflammatory factors, and enriching microbials. In addition of the anti-inflammatory effect by regulating macrophages, protection against oxidative stress with hormone secretion and oxidative-related gene expression plays a key beneficial role for the influence of IF on obese subjects.

Discussion

Physiological stress by surgery and pathophysiological disorders by endocrine diseases may be partly eased with IF. Moreover, IF might be used to treat anxiety and cognitive disorders with its cellular, metabolic and circadian mechanisms. Finally, the specific effects of IF and the mechanisms pertaining to immune system in these conditions require additional studies.
Diagnostic performance of different anthropometric indices among Iranian adolescents for intima media thickness in early adulthood: A prospective study and literature review
Authors: Asghari Golaleh , Nikparast Ali , Mahdavi Maryam , Dehghan Pooneh , Valizadeh Majid , Hosseinpanah Farhad , Azizi Fereidoun , Hadaegh Farzad
Published: February 2023

Background

There is debate regarding which anthropometric indices is the most appropriate predictor of cardiovascular disease (CVD) among adolescents. The purpose of this study was to investigate the association of body mass index (BMI), waist circumference (WC), waist-to-hip ratio (WHR), and waist-to-height ratio (WHtR) in adolescents with high carotid intima-media thickness (cIMT) in early adulthood, as the surrogate marker of CVD in a cohort study.

Methods

A total of 875 Iranian adolescents (female = 421) aged 10–17 years old were entered the study. The cIMT was measured in early adulthood (20–38 years old) after 18.2 (median) years of follow-up and defined as > 90th percentile for sex and age groups. The gender specific association between a 1-SD increase in each anthropometric measures with high cIMT was examined using multivariate logistic regression analysis adjusted for age, smoking, family history of CVD, systolic blood pressure, total cholesterol, and fasting blood sugar. In the multivariable analysis, the interaction between sex and age groups with anthropometric measures were significant (all p-values < 0.05).

Results

Among males, all anthropometric measures including BMI, WC, WHR, and WHtR were associated with high cIMT; the corresponding odds ratios were 1.43 (1.05–1.94), 1.63 (1.22–2.19), 1.33 (1.03–1.71), and 1.41 (1.07–1.87), respectively. However, after considering the related adulthood anthropometric measurements, the association remained significant for WC 1.48 (1.04–2.10) and WHR [1.28 (0.99–1.66), P = 0.06]. Moreover, among early adolescent boys aged 10–14 years, all of the anthropometric measures were significantly associated with high cIMT in the multivariate analysis that included the related adulthood anthropometric measures. The area under the curve (AUC) for the anthropometric measurements among males ranged from 0.576 for WHtR up to 0.632 for WC, without any superiority between them. Among females, only in linear regression analysis, a significant association were found between the higher value of WC and WHtR with cIMT measurement in adulthood; however, the risk reached to null after considering adult anthropometric measures.

Conclusion

General and central obesity measures were significantly associated with high cIMT only among Iranian male adolescents, the relationship that were more prominent among pre-pubertal males.
Distribution of residual tumors in esophageal squamous cell carcinoma after neoadjuvant PD-1 blockade combined with chemotherapy
Authors: Jiang Dongxian , Song Qi , Tang Han , Shi Peng , Zhang Xiaolei , Liu Yufeng , Wang Haixing , Deng Minying , Huang Jie , Su Jieakesu , Xu Chen , Tan Lijie , Hou Yingyong
Published: February 2023

Aims

The distribution of residual esophageal squamous cell carcinoma (ESCC) in the esophageal wall and resected lymph nodes was evaluated after neoadjuvant chemoimmunotherapy (nICT).

Methods and results

Clinical data were collected from 137 ESCC patients who underwent anti-programmed death 1 therapy and esophagectomy. Ninety (65.7%) achieved an major pathological response (MPR) in the esophageal wall, and 27 (19.7%) achieved an MPR in the lymph nodes. Pathologically complete response (pCR, ypT0N0) was observed in 26 patients (19%). Residual tumors located in the mucosa and/or submucosa were found in 94.6% of nonpCR patients. In the minor responders, 97.8% had residual tumor >10% in the mucosa or submucosa. A preferential regression direction toward the lumen was found in 76.4% of prepT2 nonpCR patients, or 60.7% of prepT3-4a nonpCR patients. The correlation between pCR in the esophageal wall and in lymph nodes was not significant (P=0.143). Among 19 patients with pCR in resected recurrent laryngeal nerve (RLN) lymph nodes, 31.6% had residual tumor cells in other resected lymph nodes. A significant correlation was found between ypT/ypN downstaging and tumor regression grade (P<0.05).

Conclusions

After nICT for ESCC, residual tumors were frequently found in the mucosa or submucosa, with relatively high responsiveness of the invasive front and a significant correlation with downstaging, which may help clinicians make appropriate decisions about postoperative treatment and surveillance. The differences in pCR status in primary tumors, resected lymph nodes, and RLN lymph nodes indicated the importance of assessing regression changes in all resected lymph nodes during clinical practice.
Comprehensive genomics analysis of aging related gene signature to predict the prognosis and drug resistance of colon adenocarcinoma
Authors: Feng Jubin , Fu Fengyihuan , Nie Yuqiang
Published: February 2023

Background: Colon adenocarcinoma (COAD) is a heterogeneous tumor and senescence is crucial in the occurrence of cancer. This study aimed to identify senescence-based subtypes and construct a prognostic signature to predict the prognosis and guide immunotherapy or chemotherapy decisions for COAD patients.

Methods: Based on the single-cell RNA sequencing (scRNA-seq) data of 13 samples from the Gene Expression Omnibus (GEO) database, we assessed cellular senescence characteristics. Transcriptome data, copy number variations (CNVs) and single nucleotide variations (SNVs) data were obtained from The Cancer Genome Atlas (TCGA) database. GSE39582 and GSE17537 were used for validation. Senescence subtypes were identified using unsupervised consensus clustering analysis, and a prognostic signature was developed using univariate Cox analysis and least absolute shrinkage and selection operator (LASSO). Response of risk groups to chemotherapy was predicted using the half-maximal inhibitory concentration (IC50) values. We further analyzed the relationship between risk gene expression and methylation level. The prediction performance was assessed by nomogram.

Results: Senescence-related pathways were highly enriched in malignant cells and bulk RNA-seq verified cellular senescence. Three senescence subtypes were identified, in which patients in clust3 had poorest prognosis and higher T stage, accompanied with higher tumor mutation burden (TMB) and mutations, activated inflammatory response, more immune cell infiltration, and higher immune escape tendency. A senescence-based signature using 11 genes (MFNG, GPRC5B, TNNT1, CCL22, NOXA1, PABPC1L, PCOLCE2, MID2, CPA3, HSPA1A, and CALB1) was established, and accurately predicted a lower prognosis in high risk patients. Its robustness was validated by external cohort. Low risk patients were more sensitive to small molecule drugs including Erlotinib, Sunitinib, MG-132, CGP-082996, AZ628, Sorafenib, VX-680, and Z-LLNle-CHO. Risk score was an independent prognostic factor and nomogram confirmed its reliability. Four risk genes (CALB1, CPA3, NOXA1, and TNNT1) had significant positive correlation with their methylation level, while six genes (CCL22, GPRC5B, HSPA1A, MFNG, PABPC1L, and PCOLCE2) were negatively correlated with their methylation level.

Conclusion: This study provides novel understanding of heterogeneity in COAD from the perspective of senescence, and develops signatures for prognosis prediction in COAD.
Metabolomics approach to identify key volatile aromas in Thai colored rice cultivars
Authors: Tansawat Rossarin , Jindawatt Supawat , Ekkaphan Paweena , Ruengphayak Siriphat , Vanavichit Apichart , Suttipanta Nitima , Vimolmangkang Sornkanok , De-Eknamkul Wanchai
Published: February 2023

In addition to white jasmine rice, Thailand has many native-colored rice varieties with numerous health benefits and the potential to become a global economic crop. However, the chemical characteristics of aromatic substances in native-colored rice are still mostly unknown. This study aimed to identify the key volatile aroma compounds and the biosynthetic pathways possibly involved in their formation in Thai native-colored rice varieties, and thus leading to the search for potential genetic markers for breeding colored rice with better aromatic properties. Twenty-three rice varieties in four categories: aromatic white, aromatic black, non-aromatic black, and non-aromatic red, were investigated (n=10 per variety). Seed husks were removed before the analysis of rice volatile aromas by static headspace gas chromatography–mass spectrometry. Untargeted metabolomics approach was used to discover the key volatile compounds in colored rice. Forty-eight compounds were detected. Thirty-eight of the 48 compounds significantly differed among groups at p<0.05, 28 of which at p<0.0001, with the non-aromatic black and red rice containing much lower content of most volatile constituents than the aromatic black and white rice. Focusing on the aromatic black rice, the samples appeared to contain high level of both compound groups of aldehydes (3-methylbutanal, 2-methylbutanal, 2-methylpropanal, pentanal, hexanal) and alcohols (butane-2,3-diol, pentan-1-ol, hexan-1-ol). Biosynthetically, these distinctive black-rice volatile compounds were proposed to be formed from the metabolic degradation of branched-chain amino acids (L-leucine, L-isoleucine and L-valine) and polyunsaturated fatty acids (linoleic acid and α-linolenic acid), involving the branched-chain aminotransferases and keto-acid decarboxylases and the 9-lipoxygonases and 13-lipoxygeases, respectively. The proposed degradative pathways of amino acids and fatty acids were well agreed with the profiles key volatile compounds detected in the Thai native-colored rice varieties.
The softening of Chinese digital propaganda: Evidence from the People’s Daily Weibo account during the pandemic
Authors: Zhang Chang , Zhang Dechun , Shao Hsuan Lei
Published: February 2023

Introduction

Social media infuses modern relationships with vitality and brings a series of information dissemination with subjective consciousness. Studies have indicated that official Chinese media channels are transforming their communication style from didactic hard persuasion to softened emotional management in the digital era. However, previous studies have rarely provided valid empirical evidence for the communicational transformation. The study fills the gap by providing a longitudinal time-series analysis to reveal the pattern of communication of Chinese digital Chinese official media from 2019 to 2022.

Method

The study crawler collected 43,259 posts from the People’s Daily’s Weibo account from 2019 to 2021. The study analyzed the textual data with using trained artificial intelligence models.

Results

This study explored the practices of the People’s Daily’s Weibo account from 2019 to 2021, COVID-19 is hardly normalized as it is still used as the justification for extraordinary measures in China. This study confirmed that People’s Daily’s Weibo account posts are undergoing softenization transformation, with the use of soft news, positive energy promotion, and the embedding of sentiment. Although the outburst of COVID-19 temporarily increased the media’s use of hard news, it only occur at the initial stage of the pandemic. Emotional posts occupy a nonnegligible amount of the People’s Daily Weibo content. However, the majority of posts are emotionally neutral and contribute to shaping the authoritative image of the party press.

Discussion

Overall, the People’s Daily has softened their communication style on digital platforms and used emotional mobilization, distraction, and timely information provision to balance the political logic of building an authoritative media agency and the media logic of constructing audience relevance.
Life review on psychospiritual outcomes among older adults with life-threatening illnesses: A systematic review and meta-analysis
Authors: Liu Mandong , Wang Ying , Du Yan , Chi Iris
Published: February 2023

Background

At the intersection of old age and illness, older adults with life-threatening illnesses (LTI) are a group who often show resilience and seek validation of life, acceptance, and integration of past and now, even under the fear of loss, suffering, and dying evoked by life adversities. Life review has been widely conducted to help older adults enhance well-being and cope with burdens. Spirituality is an important part of an older adult’ overall well-being, especially for those with LTI. However, few review studies examined the effectiveness of life review interventions on psychospiritual outcomes among this population. The aim of the study was to examine the effectiveness of life review on psychospiritual well-being among older adults with LTI.

Methods

A systematic review with meta-analysis following the recommendations of the Cochrane Collaboration was conducted. Database searches included PubMed, PsycINFO, the Cochrane Library, the Campbell Library, EBSCO, CNKI, and the Airiti Library up to March 2020. Gray literature and reference lists from relevant articles were also searched and reviewed.

Results

In total, 34 studies were included in the systematic review and the meta-analysis for outcomes of depression (n= 24), quality-of-life (QOL) (n= 10), anxiety (n= 5), life satisfaction (n= 3), mood (n= 3), apathy (n= 2), and general well-being (n= 2). Other psychospiritual outcome measures included spirituality, self-esteem, meaning in life, hope, and some multi-dimensional instruments. The studies greatly varied in program design, content, format, length, and more. Although with high heterogeneity, meta-analysis results demonstrated standardized mean differences in favor of life review in decreasing depression, anxiety, negative mood, and increasing positive mood and QOL compared with the control group.

Conclusion

This review calls for including more psycho-spiritual well-being measures among interventions for older adults with LTI, as well as studies with rigorous designs in future research.
Implementation of occupational safety and health measures at German schools during the SARS-CoV-2 pandemic—cross-sectional results from 31,089 teachers
Authors: Koestner Clemens , Eggert Viktoria , Dicks Theresa , Beutel Till , Kalo Kristin , Zähme Carolina , Letzel Stephan , Dietz Pavel
Published: February 2023

Background

The SARS-CoV-2 pandemic exacerbated existing health-related challenges in schools and created new ones. Under pandemic conditions, health risks increased, and with them the requirements for occupational safety and health (OSH) measures. The aim of the study was (a) to examine the status quo of OSH measures in German schools, (b) to analyze whether the implementation of OSH measures was associated with preferable outcomes and (c) to identify predictors for the implementation of OSH measures.

Methods

A nationwide cross-sectional online survey was conducted among teachers at all school types in Germany in March 2021. Data on the implementation of OSH measures (risk assessments, infection protection instructions and instructions on occupational safety), associated health-related parameters (e.g., somatic symptoms, PHQ-15) and predictor variables (e.g., gender, age or federal states) were assessed using descriptive statistics, analysis of variance and multiple linear regression analysis.

Results

Less than 10% of surveyed teachers (N = 31,089) reported that their schools met legal requirements for occupational safety and health measures. Beneficial associations became apparent where more measures were implemented, e.g., significantly better somatic and mental health. Predictors for the implementation of OSH measures were found, especially on a systemic level (e.g., federal states schools were located in).

Conclusions

Our study can serve as a basis for future studies. It provides a status quo regarding the implementation of, associations with and predictors for OSH measures in German schools. Our results are best understood as evidence-based arguments to encourage political decision makers to improve the implementation of OSH measures in German schools and thereby foster teachers' health.
Multi-Objective Design Optimization of Multiple Tuned Mass Dampers for Attenuation of Dynamic Aeroelastic Response of Aerospace Structures
Authors: Gasparetto Victor E. L. , Reid Jackson , Parsons William P. , ElSayed Mostafa S. A. , Saad Mohamed , Shieldand Stephen , Brown Gary L. , Hilliard Lawrence M.
Published: February 2023

This paper proposes a design procedure to determine the optimal configuration of multi-degrees of freedom (MDOF) multiple tuned mass dampers (MTMD) to mitigate the global dynamic aeroelastic response of aerospace structures. The computation of the aerodynamic excitations is performed considering two models of atmospheric disturbances, namely, the Power Spectral Density (PSD) modelled with the Davenport Spectrum (DS) and the Tuned Discrete Gust (TDG) with the one-minus cosine profile. In order to determine the optimum sets of MTMD, a Multi-objective design Optimization considering Genetic Algorithm (MOGA) is implemented, where the selected fitness functions for the analysis are the minimization of the total mass of the resonators as well as the concurrent minimization of the peak displacements of a specified structural node in all translational degrees of freedom. A case study is presented to demonstrate the proposed methodology, where the optimal sets of MTMD are determined for the concurrent minimization of the pointing error of a truss-like antenna structure as well as the mass of the considered MTMD. It is found that the placement of the MTMD in the primary reflector of the antenna structure provided a maximum reduction in the pointing error of 62.0% and 39.2%, considering the PSD and the TDG models, respectively. Finally, this paper presents an advanced framework to estimate optimal parameters of MTMD control devices under convoluted loading cases as an initial step towards the use of such passive systems in applications that commonly employ active or semi-active solutions.
QTL Mapping for Fiber Quality Based on Introgression Lines Population from G. hirsutum × G. tomentosum
Authors: Chang Xinyi , Guo Chunping , Pan Zhenyuan , Wu Yuanlong , Shen Chao , Chao Lei , Shui Guangling , You Chunyuan , Xu Jianwei , Lin Zhongxu , Nie Xinhui
Published: February 2023

As one of the most widely cultivated cotton species in China, upland cotton has moderate fiber quality and wide applicability, but its genetic basis is relatively narrow. To expand genetic diversity and improve fiber quality, in this study an introgression population (BC5S5) containing 107 lines was constructed by using G. hirsutum acc. 4105 as the recurrent parent and G. tomentosum as the donor parent. Using the specific-locus amplified fragment sequencing (SLAF-seq) strategy, 3157 high-throughput single nucleotide polymorphism (SNP) markers were obtained. Linkage analysis showed that a total of ninety-one QTLs related to fiber quality traits were detected in three environments, and the phenotypic variance explained (PVE) rates were 4.53–20.92%. Forty-six QTL (50.55%) synergistic genes were derived from G. tomentosum. Among them, qFS-A02-1 and qSCI-A02-1 were stably detected with a PVE of 9.8–16.71% and 14.78–20.92%, respectively. Within the candidate interval, Ghir_A02G012730, Ghir_A02G012790 and Ghir_A02G012830 were found to be possibly involved in cellulose and cell wall biosynthesis, with a relatively high expression during fiber development, 20 DPA and 25 DPA, which suggested that these three genes may be involved in the regulation of fiber strength traits, but their functions need further validation to determine the regulatory mechanism. Our research lays the foundation of fiber quality related to basic genetic research and breeding in cotton.
Assessing Financial Literacy and Farmland Abandonment Relationship in Ghana
Authors: Ankrah Twumasi Martinson , Dogbe Bright Senyo , Ankrah Ernest Kwarko , Ding Zhao , Jiang Yuansheng
Published: February 2023

Farmland abandonment has been a major concern for policymakers in most developing nations since it is associated with food security and poverty alleviation. In view of this, assessing its potential determinants is essential and timely. This study examines the relationship between financial literacy and farmland abandonment in Ghana using survey data (N = 572). The study employs endogenous switching regression (ESR) for its estimation. Our findings show that financial literacy is low among rural dwellers. Also, the findings depict that financial literacy is positively related to farmland abandonment reduction. Moreover, different household groups depict a heterogeneous relationship between financial literacy and farmland abandonment. Thus, the association between financial literacy and farmland abandonment reduction is more pronounced for low-income farm households and female farmers. We recommended that financial literacy programs can be organized or shown on national radios and television to provide financial education to the country’s residents. Our findings could offer some implications for stimulating agricultural intensification while ensuring rural advancements.
Behavioral Response of a Specialist Predator, Stethorus gilvifrons (Coccinellidae: Coleoptera), to Cope with Web Structures of Four Different Spider Mite Pest Species
Authors: Mirza Jawwad Hassan , Kamran Muhammad , Alatawi Fahad Jaber
Published: February 2023

The present study aimed to evaluate the web coping and attack behavior of a specialist mite predator, Stethorus gilvifrons, against the webbing structures of different spider mite pest species: Oligonychus afrasiaticus, Tetranychus urticae, Eutetranychus orientalis and O. punicae. The females of each pest species, in three different treatments, were allowed to construct web structures in the experimental arenas. The predator exhibited three different attack behaviors which were associated with the webbing life types of pest species. Against the O. afrasiaticus web, the predator used its palps and mandibles. Against the T. urticae web, the predator also made use of its first pair of legs to widen the entry hole it made by cutting. Against the O. punicae web, the predator protruded its hypognathous mouthparts to feed on mite individuals by merely entering into the web. Behavioral responses were also found to be associated with webbing life types of pests. Against O. afrasiaticus and O. punicae, the predator spent much time in web penetration and searching behaviors, respectively. The density-dependent complexity of web structure may cause hindrance to the predator, and it is suggested that adults should be released when a pest colony is growing with low webbing complexity for efficient pest management.
Evaluation of Cutting Stability of a Natural-Rubber-Tapping Robot
Authors: Zhou Hang , Gao Jin , Zhang Fan , Zhang Junxiong , Wang Song , Zhang Chunlong , Li Wei
Published: February 2023

Natural rubber is a crucial raw material in modern society. However, the process of latex acquisition has long depended on manual cutting operations. The mechanization and automation of rubber-tapping activities is a promising field. Rubber-tapping operations rely on the horizontal cutting of the leading edge and vertical stripping of the secondary edge. Nevertheless, variations in the impact acceleration of the blade can lead to changes in the continuity of the chip, affecting the stability of the cut. In this study, an inertial measurement unit (IMU) and a robotic arm were combined to achieve the real-time sensing of the blade’s posture and position. The accelerations of the blade were measured at 21 interpolated points in the optimized cutting trajectory based on the principle of temporal synchronization. A multiple regression model was used to establish a link between impact acceleration and chip characteristics to evaluate cutting stability. The R-squared value for the regression equation was 0.976, while the correlation analysis for the R-squared and root mean square error (RMSE) values yielded 0.977 and 0.0766 mm, respectively. The correlation coefficient for the Z-axis was the highest among the three axes, at 0.22937. Strict control of blade chatter in the radial direction is necessary to improve the stability of the cut. This study provides theoretical support and operational reference for subsequent work on end-effector improvement and motion control. The optimized robotic system for rubber tapping can contribute to accelerating the mechanization of latex harvesting.
Effect of Soil Agricultural Use on Particle-Size Distribution in Young Glacial Landscape Slopes
Authors: Sowiński Paweł , Smólczyński Sławomir , Orzechowski Mirosław , Kalisz Barbara , Bieniek Arkadiusz
Published: February 2023

In the literature, mainly particle-size distribution (PSD) analyses in the soil catenas, of e.g., moraine and riverine landscapes were discussed. Analysis and comparison of PSD in moraine (ML) and ice-dammed lakes (ID-LL) landscapes were not studied. Since the landscape of ice-dammed lakes origin has diversified relief and is under intensive agricultural use, the aspects of erosion are of great importance. The changes in PSD were studied in 14 soil catenas (toposequences) of eroding soils at the upper slope (US) as well as colluvial soils at the middle (MS) and lower (LS) slopes and in the depressions (D). The PSD of the fine fractions (<2 mm) was analysed according to the hydrometer method. In order to describe the effect of agricultural use on the variability of PSD in soil surface horizons, sedimentological and granulometric indices were calculated. In the studied moraine landscape, the content of coarse silt fraction was increasing in the catenal sequence from 9.7% in the US to 17.7% in the D. Similar relationships were revealed for the fine silt content. Significant differences were found between the average contents of coarse and fine silt fractions at the US as well as the LS and the D. However, such a relation was not found in the soil catena in the ice-dammed lake landscape. Eroded and colluvial soil materials were very poorly sorted with a standard deviation index of 2.65–3.69. Humus horizons of analysed soils had very fine, fine skewed PSD, mesokurtic and platykurtic distribution (ML), symmetrical, fine skewed and platykurtic distribution (I-DLL). The cluster analysis enabled the separation of two groups of soils: one group in the moraine landscape and the other in the ice-dammed lakes landscape. The PSD in studied soils was similar only among the soils within one type of landscape.
The Value of Crop Diversification: Understanding the Factors Influencing Consumers’ WTP for Pasta from Sustainable Agriculture
Authors: Rossi Eleonora Sofia , Zabala José A. , Caracciolo Francesco , Blasi Emanuele
Published: February 2023

(1) Background: The pressure that agricultural systems’ intensive management exerts on the environment concerns society. For this reason, the demand for sustainable foods is growing in the market. This study investigated consumers’ Willingness To Pay (WTP) for dry semolina pasta produced with raw materials from more diversified agricultural systems and which factors influence this choice. (2) Methods: The data, collected through a contingent valuation exercise, involved 185 Italian consumers. Through a Tobit model, the drivers influencing the WTP were selected. A latent class cluster analysis determined four different groups of consumers. (3) Results: The data highlighted a real social demand for sustainability. Consumers recognize a higher WTP for sustainable pasta. This value is influenced by drivers such as purchasing habits, personal characteristics, and environmental attitudes. (4) Conclusions: This work offers an approach, both from a market and political point of view, to how this higher potential WTP could be identified and redistributed among the value chain actors by structuring both marketing strategies for the different types of consumers and political tools able to help agri-food chains towards sustainability transition.
Multi-Node Path Planning of Electric Tractor Based on Improved Whale Optimization Algorithm and Ant Colony Algorithm
Authors: Liang Chuandong , Pan Kui , Zhao Mi , Lu Min
Published: February 2023

Under the “Double Carbon” background, the development of green agricultural machinery is very fast. An important factor that determines the performance of electric farm machinery is the endurance capacity, which is directly related to the running path of farm machinery. The optimized driving path can reduce the operating loss and extend the mileage of agricultural machinery, then multi-node path planning helps to improve the working efficiency of electric tractors. Ant Colony Optimization (ACO) is often used to solve multi-node path planning problems. However, ACO has some problems, such as poor global search ability, few initial pheromones, poor convergence, and weak optimization ability, which is not conducive to obtaining the optimal path. This paper proposes a multi-node path planning algorithm based on Improved Whale Optimized ACO, named IWOA-ACO. The algorithm first introduces reverse learning strategy, nonlinear convergence factor, and adaptive inertia weight factor to improve the global and local convergence ability. Then, an appropriate evaluation function is designed to evaluate the solving process and obtain the best fitting parameters of ACO. Finally, the optimal objective function, fast convergence, and stable operation requirements are achieved through the best fitting parameters to obtain the global path optimization. The simulation results show that in flat environment, the length and energy consumption of IWOA-ACO planned path are the same as those of PSO-ACO, and are 0.61% less than those of WOA-ACO. In addition, in bump environment, the length and energy consumption of IWOA-ACO planned path are 1.91% and 4.32% less than those of PSO-ACO, and are 1.95% and 1.25% less than those of WOA-ACO. Therefore, it is helpful to improve the operating efficiency along with the endurance of electric tractors, which has practical application value.
Effect of the Different Fertilization Treatments Application on Paddy Soil Enzyme Activities and Bacterial Community Composition
Authors: Xiao Qingqing , He Boping , Wang Su
Published: February 2023

The application amount of fertilizer is believed to dramatically influence soil bacteria in paddy fields, and soil bacteria critically affect soil enzyme activities and the growth of rice in paddy fields. Thus, providing a suitable amount of fertilization to ensure rice yields is an important issue in field management. In this study, four treatments were carried out in a paddy field, including control (fertilization methods commonly used by farmers in the past), IT + RF 10, IT + RF 20, and IT + RF 30 (integrated technology and 10%, 20%, and 30% reduction in nitrogen fertilizer, respectively). Soil samples were collected in two periods, the rice booting stage and the harvest period. High-throughput sequencing was used for analysis of soil bacterial diversity and community composition across different fertilizer treatments, and clarified the effects of different fertilizer treatments on soil enzyme activities and bacterial community composition. This study showed that a reduction in chemical fertilizer application has a certain impact on the soil pH, total nitrogen (TN), total phosphorus (TP), soil organic matter (SOM) value, and bacterial community of the rice planting system, and that the IT + RF 10 treatment was the best way to reduce fertilizer application, which can reduce nutrient loss in the paddy soil. The application of organic fertilizer partially replaces chemical fertilizer, which not only effectively stimulates soil enzyme activity, but also enriches bacterial groups that may participate in complex organic matter decomposition and soil nutrient mobilization.
Secondary Metabolites of Pathogenic Fungi in Triticum durum Grain Protected with Debaryomyces hansenii in Two Different Locations in Poland
Authors: Wachowska Urszula , Sulyok Michael , Wiwart Marian , Suchowilska Elżbieta , Giedrojć Weronika , Gontarz Dariusz , Kandler Wolfgang , Krska Rudolf
Published: February 2023

Durum wheat grain can accumulate mycotoxins because it is highly sensitive to infections caused by pathogens of the genera Fusarium and Alternaria. Reduced fungicide use increases the demand for biological methods of pathogen control. The aim of the experiment was to evaluate the efficacy of Debaryomyces hansenii (Dh) yeast in reducing the content of secondary fungal metabolites present in the spikes of five durum wheat cultivars grown in southern and northern Poland. A total of 27 Fusarium metabolites and nine metabolites produced by other fungi were identified in the grain. The application of the Dh yeast strain decreased deoxynivalenol concentration in all samples relative to control treatments (by 14–100%) and treatments inoculated with F. graminearum (by 23–100%). In northern Poland, the biological treatment also led to a considerable reduction in the content of culmorin (by 83.2–100%) and enniatins A1 and B (by 9.5–65.3% and 6.7–70%, respectively) in the grain. An analysis of multiple fungal metabolites is a highly useful tool for determining grain quality and its suitability for consumption. When applied in the flowering stage, yeasts can partly complete fungicides in reducing Fusarium head blight.
Establishing Optimal Planting Windows for Contrasting Sorghum Cultivars across Diverse Agro-Ecologies of North-Eastern Nigeria: A Modelling Approach
Authors: Akinseye Folorunso M. , Ajeigbe Hakeem A. , Kamara Alpha Y. , Omotayo Akinrotimi O. , Tofa Abdullahi I. , Whitbread Anthony M.
Published: February 2023

In the context of climate change, the sowing date and cultivar choice can influence the productivity of sorghum, especially where production is constrained by low soil fertility and early terminal drought across the challenging agro-ecologies of north-eastern Nigeria. Planting within an optimal sowing window to fit the cultivar’s maturity length is critical for maximizing/increasing the crop yield following the appropriate climate-smart management practices. In this study, the APSIM crop model was calibrated and validated to simulate the growth and yield of sorghum cultivars with differing maturing periods sown within varying planting time windows under improved agricultural practices. The model was run to simulate long-term crop performance from 1985 to 2010 to determine the optimal planting windows (PWs) and most suitable cultivars across different agro-ecological zones (AEZs). The performance of the model, validated with the observed farm-level grain yield, was satisfactory across all planting dates and cropping systems. The model predicted a lower mean bias error (MBE), either positive or negative, under the sole cropping system in the July sowing month compared to in the June and August sowing months. The seasonal climate simulations across sites and AEZs suggested increased yields when using adapted sorghum cultivars based on the average grain yield threshold of ≥1500 kgha−1 against the national average of 1160 kgha−1. In the Sudan Savanna (SS), the predicted optimum PWs ranged from 25 May to 30 June for CSR01 and Samsorg-44, while the PWs could be extended to 10 July for ICSV400 and Improved Deko. In the Northern Guinea Savanna (NGS) and Southern Guinea Savanna (SGS), the optimal PWs ranged from 25 May to 10 July for all cultivars except for SK5912, for which predicted optimal PWs ranged from 25 May to 30 June. In the NGS zone, all cultivars were found to be suitable for cultivation with exception of SK5912. Meanwhile, in the SGS zone, the simulated yield below the threshold (1500 kgha−1) could be explained by the sandy soil and the very low soil fertility observed there. It was concluded that farm decisions to plant within the predicted optimal PWs alongside the use of adapted sorghum cultivars would serve as key adaptation strategies for increasing the sorghum productivity in the three AEZs.
Dairy Cows Offered Fresh Chicory Instead of Ensiled Pasture during an Acute Heat Challenge Produced More Milk and Had Lower Body Temperatures
Authors: Williams S. Richard O. , Moate Peter J. , Garner Josie B. , Hannah Murray C. , Giri Khageswor , Wales William J. , Marett Leah C.
Published: February 2023

The frequency, duration, and intensity of heat waves in Australia are increasing. To reduce the impact of heat waves on milk production, novel management strategies are required. Altering the forage type and amount offered affect the heat load on dairy cows and offer potential strategies to ameliorate the effects of hot weather. Thirty-two multiparous, lactating Holstein–Friesian cows were assigned one of four dietary treatments: chicory high amount, chicory low amount, pasture silage high amount, or pasture silage low amount. These cows were exposed to a heat wave in controlled-environment chambers. Cows that were offered fresh chicory had similar feed intake to cows that were offered pasture silage (15.3 kg DM/d). However, cows that were offered chicory produced greater energy-corrected milk (21.9 vs. 17.2 kg/d) and had a lower maximum body temperature (39.4 vs. 39.6 °C) than cows that were offered pasture silage overall. Cows that were offered the high amount of forage had greater feed intake (16.5 vs. 14.1 kg DM/d) and energy corrected milk yield (20.0 vs. 17.9 kg/d) than cows that were offered the low amount, as intended, but with no difference in maximum body temperature (39.5 °C). We conclude that feeding chicory instead of pasture silage to dairy cows shows promise as a dietary strategy to ameliorate the effect of heat exposure, and there was no advantage in restricting feed amount.
Comparison of Fecal Microbiota Communities between Primiparous and Multiparous Cows during Non-Pregnancy and Pregnancy
Authors: Jia Xianbo , He Yang , Kang Zhe , Chen Shiyi , Sun Wenqiang , Wang Jie , Lai Songjia
Published: February 2023

Imbalances in the gut microbiota composition may lead to several reproductive disorders and diseases during pregnancy. This study investigates the fecal microbiome composition between primiparous and multiparous cows during non-pregnancy and pregnancy to analyze the host-microbial balance at different stages. The fecal samples obtained from six cows before their first pregnancy (BG), six cows during their first pregnancy (FT), six open cows with more than three lactations (DCNP), and six pregnant cows with more than three lactations (DCP) were subjected to 16S rRNA sequencing, and a differential analysis of the fecal microbiota composition was performed. The three most abundant phyla in fecal microbiota were Firmicutes (48.68%), Bacteroidetes (34.45%), and Euryarchaeota (15.42%). There are 11 genera with more than 1.0% abundance at the genus level. Both alpha diversity and beta diversity showed significant differences among the four groups (p < 0.05). Further, primiparous women were associated with a profound alteration of the fecal microbiota. The most representative taxa included Rikenellaceae_RC9_gut_group, Prevotellaceae_UCG_003, Christensenellaceae_R_7_group, Ruminococcaceae UCG-005, Ruminococcaceae UCG-013, Ruminococcaceae UCG-014, Methanobrevibacter, and [Eubacterium] coprostanoligenes group, which were associated with energy metabolism and inflammation. The findings indicate that host-microbial interactions promote adaptation to pregnancy and will benefit the development of probiotics or fecal transplantation for treating dysbiosis and preventing disease development during pregnancy.