bluuebunny/crossref_metadata_2025 huggingface dataset:
Laser therapy in the complex treatment of patients with compression-ischemic neuropathy
Authors: Shpagin M. V. , Yarikov A. V. , Pavlov S. S. , Sokolov A. A.
Published: August 2021

Material and methods. Twenty-two patients with compression-ischemic neuropathy were included into the study. Low-level laser device “ALOK-1” was used for laser therapy. Its beam impacts tissues directly; its power is 1 mW, wavelength – 0.63 μm, spot diameter – 0.8 mkm.

Results. Subjective, objective and instrumental findings became significantly better, if to compare with the pre-operative period.

Conclusion. The developed technique has shown its good efficiency. The intraoperative laser therapy CIN patients decreased the severity of symptoms up to their complete regression; patients were highly satisfied with their postoperative outcomes.
Photodynamic therapy for periprosthetic joint infection
Authors: Guseynov A. I. , Baranov А. V. , Radzhabov A. A. , Derbenev V. A. , Karandashov V. I. , Alexandrova N. P.
Published: August 2021

Introduction. The increase in the number of patients with joint and hip diseases is an actual problem in the clinical medicine. Objective: to improve outcomes of the combined surgical treatment of patients with deep periprosthetic infection (PPI) by developing and improving surgical techniques using spacers and laser technologies.

Material and methods. Thirty-five patients with suppurated large joints as a complication after their replacement were examined and treated. In 9 (25.7 %) patients, PPI developed within 3–12 months after the primary arthroplasty. Twenty (57.1 %) patients developed PPI within 1–2 years after the surgery. In 4 (11.4 %) cases, suppuration in the endoprosthetic area developed in 2–3 years, and in 2 (5.7 %) patients – in 3–3.5 years. The age of the patients was 47–70 years. The main group consisted of 20 patients, the control group – of 15 patients. Patients from the main group had laser photodynamic therapy (PDT) session after the removal of endoprosthetic elements and devitalized tissues.

Results. Patients from the main group had uneventful postoperative course – less pain syndrome, rapid resolution of the infl ammatory process, wound healing by the primary tension.

Conclusion. Application of a new intraoperative PDT technique, developed by the authors, promotes rapid resolution of purulent-infl ammatory process and better healing of postoperative wounds by primary tension.
Importancia de las características socio-demográficas y epidemiológicas en el manejo de los tumores de ovario
Authors: Guerra Jean Carlos , Calderaro Di Ruggiero Franco
Published: August 2021

Objetivo: Describir las principales características socio-demográficas y epidemiológicas, de las pacientes con tumores de ovarios. Métodos: Estudio descriptivo, transversal, con una población de 133 pacientes que acudieron a la consulta de Ginecología Oncológica, del Servicio Oncológico Hospitalario del IVSS, entre julio de 2016 y julio de 2017, con diagnóstico de tumores de ovarios, donde solo 24 cumplieron con los criterios de inclusión. Resultados: Mayor procedencia Estado Miranda con 42%. La raza mestiza 46%. El promedio de edad 48,33 años. Menarquia promedio 12,29 años. Sexarquia promedio de 19,14 años. Parejas sexuales entre 1-2, 46%. Antecedentes obstétricos: 2,5 gestaciones. Anticonceptivos orales 21%. Dispositivos intrauterinos en 21%. Hábito tabáquico 29%. Síntomas en el 83% (58% aumento de volumen abdominal) con evolución de 4,05 meses. 63% tumores benignos (cistoadenoma mucinoso con 46%), 33% tumores malignos (adenocarcinoma endometroide con 37%) y 4% tumor borderline (atípicamente proliferativo), el tipo (mucinoso). Según la clasificación FIGO estadio 1C y 3C con 38% respectivamente. La lateralidad predominante lado derecho (54%). El promedio del tamaño fue de 17,60 cm. Conclusión: Las características socio-demográficas, epidemiológicas y clínico-patológicas, determinan el manejo adecuado de los tumores de ovarios, por lo que deben ser investigadas a fondo, y de esta manera, ofrecer el mejor tratamiento individualizado a las pacientes.
Bloqueos caudales en pacientes pediátricos
Authors: Chirinos Alfredo , Briones Jimmy , Córdova César , Mediavilla Jeanette
Published: August 2021

En esta investigación se caracteriza el comportamiento hemodinámico de los bloqueos caudales pediátricos sometidos a cirugías de hemiabdomen inferior y región inguinal, en el Hospital General Docente de Calderón (HGDC), Quito-Ecuador, en el periodo junio-diciembre de 2019; además se describen las características demográficas de la población en estudio, determinando la intensidad del dolor con la escala visual análoga (EVA). Asimismo, se reportan los cambios hemodinámicos en los diferentes tiempos establecidos en el estudio, verificando el uso de analgésicos de rescate y la búsqueda de efectos adversos con la utilización de esta técnica. Se trata de un estudio descriptivo retrospectivo, considerándose la totalidad de la población de estudio (32 pacientes). El análisis estadístico se realizó con medidas de frecuencia y tendencia central (mediana), de dispersión (desviación estándar, rango). Con respecto, a los resultados de la EVA, es importante destacar que a pesar de dar una diferencia estadísticamente significativa (p<0,05), en la práctica médica esta escala se mantiene debajo de 1 punto, es decir, dolor leve; las variables hemodinámicas se mantuvieron estables con respecto al pre bloqueo. Con el uso de analgésicos de rescate, se reportó solo un paciente 3,1 %, que se le administro dosis de rescate. Los efectos adversos reportados con el bloqueo caudal fueron: náuseas 6,3 % somnolencia 3,1 %, y ningún efecto con 90,6 % respectivamente. El bloqueo caudal proporciona una analgesia excelente en el postoperatorio según la EVA en los diferentes tiempos en el estudio.
Hígado agudo graso del embarazo. Reporte de caso
Authors: Lara Dicxander , Díaz Alejandra , Rivero Alexandra , Romero Marvina , Sarmiento Carmen , Orfila Josefa
Published: August 2021

El hígado graso del embarazo es una patología poco frecuente en la especialidad obstétrica, cuyo diagnóstico se realiza basado en los criterios de Swansea, muchas veces es un diagnóstico que se realiza por exclusión; usualmente se presenta entre las semanas 30 y 35 del embarazo, y la cura definitiva se realiza con la interrupción expedita del mismo; con una tasa de recuperación casi del 100% si se realiza la interrupción oportuna y una tasa de mortalidad materno fetal actual del 10%. Es importante estar atentos a la ganancia ponderal de la embarazada durante el control prenatal, la epigastralgia, y los signos clínicos asociados a hipoglicemia.
Sala de emergencias Bagdad. La evolución en la cirugía de control de daños
Authors: Valls Puig Juan Carlos , Urra Eduardo , Blanco Aníbal
Published: August 2021

Las características de las heridas producidas en los atentados terroristas en varias ciudades del mundo y en los combates de las operaciones militares en Afganistán e Irak, han determinado un nuevo tipo de manejo de las lesiones por parte del personal medico involucrado. La categorización de las hemorragias, el nuevo esquema de atención prehospitalaria MARCH y la incorporación de la resucitación balanceada a la cirugía de control de daños, en un hospital de soporte en combate en Bagdad, representan parte del cambio. La retroalimentación entre la experiencia civil y militar ha permitido el progreso del tratamiento de los traumatismos. Los hospitales venezolanos han desarrollado su propia experiencia en el desarrollo de la cirugía de control de daños. Un nuevo tipo de ingresos en las emergencias capitalinas y la covid 19 señalan los nuevos desafíos a los que se enfrentan los cirujanos venezolanos.
Exercise/Resistance Training and Muscle Stem Cells
Authors: Fukada So-ichiro , Nakamura Ayasa
Published: August 2021

Skeletal muscle has attracted attention as endocrine organ, because exercise-dependent cytokines called myokines/exerkines are released from skeletal muscle and are involved in systemic functions. While, local mechanical loading to skeletal muscle by exercise or resistance training alters myofiber type and size and myonuclear number. Skeletal muscle-resident stem cells, known as muscle satellite cells (MuSCs), are responsible for the increased number of myonuclei. Under steady conditions, MuSCs are maintained in a mitotically quiescent state but exit from that state and start to proliferate in response to high physical activity. Alterations in MuSC behavior occur when myofibers are damaged, but the lethal damage to myofibers does not seem to evoke mechanical loading-dependent MuSC activation and proliferation. Given that MuSCs proliferate without damage, it is unclear how the different behaviors of MuSCs are controlled by different physical activities. Recent studies demonstrated that myonuclear number reflects the size of myofibers; hence, it is crucial to know the properties of MuSCs and the mechanism of myonuclear accretion by MuSCs. In addition, the elucidation of mechanical load-dependent changes in muscle resident cells, including MuSCs, will be necessary for the discovery of new myokines/exerkines and understating skeletal muscle diseases.
Cadernos Brasil na OCDE : investimentos
Authors: Thorstensen Vera Helena
Published: August 2021

Na OCDE, o tema dos investimentos é discutido no âmbito do Comitê de Investimentos, criado em 2004, após fusão entre o Comitê de Investimento Estrangeiro e Empresas Multinacionais e o Comitê sobre Movimentos de Capitais e Transações Invisíveis. O Comitê de Investimentos tem o objetivo de discutir, elaborar e propor políticas que auxiliem seus membros a encontrar formas de usufruir dos benefícios de fluxos livres de investimento internacional, mediante adoção de regras transparentes e não discriminatórias, que também garantam a segurança nacional e o interesse público.
TD 2675 - Agricultura nordestina: análise comparativa entre os censos agropecuários de 2006 e 2017
Authors: Castro César Nunes de , Pereira Caroline Nascimento
Published: August 2021

A agricultura nordestina, comparativamente ao restante do país, possui um histórico de baixa produtividade e pouco uso de inovações tecnológicas. Este cenário é fruto de políticas públicas que não favoreceram na mesma medida o Nordeste e o Centro-Sul do Brasil. Investimentos pontuais foram realizados ao longo dos anos, como perímetros irrigados, arranjos produtivos locais, entre outras ações focalizadas que produziram crescimento concentrado. Deste modo, é importante analisar com base em dados do
censo agropecuário como a agricultura nordestina e o uso das inovações tecnológicas se comportaram nos últimos levantamentos dos Censos Agropecuários de 2006 e 2017. A hipótese do trabalho é que houve crescimento produtivo e maior adoção de tecnologias por parte dos produtores. Entretanto, apesar do crescimento agrícola e do maior uso de tecnologias, o crescimento continua focalizado e concentrado em algumas poucas áreas, como o polo de fruticultura Petrolina-Juazeiro e a área de expansão agrícola do cerrado – Matopiba.
Diarrhetic shellfish poisoning toxin profile of shellfish from Southern Atlantic coasts of Morocco
Authors: Abouabdellaha Rachid , Bennouna Asmae , Attar Jaouad El , Erler Katrin , Dellal Mina , Chafik Abdel ghani , Moukrim Abdelatif
Published: August 2021

During the monitoring program of phycotoxins conducted in 2005 and 2006, lipophilic shellÃ‚Â fish toxins (LSTs) are involved in shellfish toxicity phenomena in the South Atlantic Moroccan coasts (Dakhla region). Toxicity was assessed by the traditional mouse bioassay (MBA); the content and the nature of the toxic components were established through Liquid chromatography (LC) coupled with mass spectrometry (MS). The Ã¢â‚¬ËœtraditionalÃ¢â‚¬â„¢ DSP toxins group, okadaic acid (OA) and dinophysitoxins (DTXs) and their associated esters were exclusives contaminants of DakhlaÃ¢â‚¬â„¢s shellfish (mussels, cockles, oysters and solen). Pectenotoxins, yessotoxins, and azaspiracids were not detected during this study. A survey of the phytoplankton community in the surrounding seawater revealed the presence of several potentially toxic species from the Dinophysis genus.
Microbial spectrum and antibiotic susceptibility of uropathogens causing Urinary tract infection in pregnant women
Authors: Jayavanth Pallavi , Hamid Junainah Abd , Mohan Mathan , Rafi Mohamed , Anamalley Rajasegar , Harun Haniza
Published: August 2021

Urinary tract infection (UTI) is an intermittent cause of sickness in the pregnant women. A study was conducted to identify the microbial gamut and antibiotic vulnerability patterns of bacteria isolated from pregnant women with suspected urinary tract infection. In total, 75 urine specimens were screened for bacteriuria over a period of four months. Aseptically collected urine specimens were subjected to standard isolation and characterization procedures. Antibiotic susceptibility test was performed according to CLSI (2007). Five types of bacteria were isolated namely Escherichia coli 32(55%), Staphylococcus aureus 12 (21%), Enterococcus Sp 7 (12%), Klebseilla pneumoniae 4 (7%) and Pseudomonas aeruginosa 3 (5%). All bacterial isolates exhibited efficacy to all the antibiotics namely fosfomycin (50mg), ciprofloxacin (10mg), trimethoprim & sulfamethoxazole (25 mg), ampicillin (10 mg), nitrofurantoin (300 mg) and cefazolin (30mg) except amoxycillin. Ampicillin and ciprofloxacin exhibited illustrious efficacy at low concentration. Regular antimicrobial surveillance and reinforcement of appropriate antibiotic regimen in pregnant women with UTI will restrain the reÃ¢â‚¬Âemergence of antibiotic resistant uropathogens.
Inhibition of platelet aggregation and stimulation of natural killer cells functionality by administration of flavonoids
Authors: Theoharis Vasilis , Toliopoulos Ioannis , Simos Yannis , Metsios Apostolos , Zelovitis Ioannis , Tzora Athina , Liasko Roman , Skoufos Ioannis , Karkabounas Spiros
Published: August 2021

Platelets play an important role in homeostasis, inflammation and regulation of immune response. Natural Killer cells (NKC) on the other hand are the first line of defense of the immune system against viruses, bacteria and cancer cells. Platelets directly protect tumor cells from NK lysis. Thus, platelet aggregation around migrating cancer cells as well as the response of the immune system constitutes important mechanisms, which influence the progression of malignant diseases. The present study was designed to evaluate the possible effects of flavonoids (genistein, apigenin and quercetin) on the stimulation of the immune system and the inhibition of platelet aggregation. Two experimental protocols were used: a)Ã‚Â ex vivo aggregation of human platelets, production of thromboxane A2 (TXA2) and estimation of the expression of the platelet membranic receptor GpIIb/IIIa, and b) functional activity of human NK lymphocytes against K562 target cells in vitro. All substances were found to induce a dose dependent inhibition of platelet aggregation and to reduce production of TXA2 in platelets. There was a decrease in the number of the GpIIb/IIIa receptors on the platelets surface membrane. Flow cytometry analysis revealed that all substances powerfully reinforce cytotoxic activity of NKC against K562 cells. These results show that flavonoids increase the susceptibility of tumor cells to NK cells by decreasing platelet aggregation and stimulating the NK lymphocyte activity.
Molecular Characterization of Campylobacter jejuni in Raw Poultry Meat and Ready-to-Eat Poultry Meat Products in Northern India
Authors: Sharma Gagan , Rathore R. Swaroop , Chauhan U. Kumari , Chauhan Singh R. Swaroop
Published: August 2021

Campylobacter jejuni  Ã¢â‚¬Â toÃ¢â‚¬Â eat poultry meat products have been foundimportant source of transmission of this organism on consumption. In thecurrent study,raw poultry and ready to eat poultry meat product samples atBareilly part of Northern India were tested for  C. jejuni. Total 660 samples ofpoultry meat and ready  Ã¢â‚¬Â to Ã¢â‚¬Âeat poultry meat products were screened for thepresence of  C. jejuni using both cultural methods as well as PCR assay. Out ofthe total samples, 126 (19.09%) were confirmed positive by cultural methods,while 149 (22.50%) were confirmed positive by PCR assay. There wasalso variation in the prevalence of  C. jejuni amongst the various readyÃ¢â‚¬Â toeatpoultry meat products. This study indicates the need for risk assessmentbased on microbiological criteria for  C .jejuni in poultry meat and readyÃ¢â‚¬Â toeatpoultry meat products with strict implementation of provision of HazardAnalysis and Critical Control Point (HACCP) system. has been emerged as an important food borne zoonoticbacterial pathogen associated with gastric illness in human beings. Contaminatedpoultry and ready
Evaluation of leaf and vine powders of Secamone afzelii (Schult) K. Schum for control of Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae) in stored cowpea Vigna unguiculata (L.) Walp.
Authors: Adesina J. M. , Ofuya T. I.
Published: August 2021

The experiment was carried out to evaluate the efficacy of Secamone afzeliileaf and vine powders applied at the rate of 0.5g, 1.0g, 1.5g and 2.0g underambient laboratory conditions on Callosobruchus maculatus on stored cowpea.Mortality of adult insects at 48 h post treatment, number of eggs laid,adult emergence, adult exit holes and percentage weight loss in each treatmentwere compared with those of control. The results indicated that vinepowder caused significantly greater kill (P < 0.05) of C. maculatus adultswhen applied at 2.0g than other dosages. The application rate of 2.0g of leafpowder was able to cause reduction in number of eggs laid by the weevil(5.67/20g seed) and significantly (P < 0.05) suppressed adult emergence(21.12). Adult exit holes and percentage weight loss were significantly reducedat all levels of application. It is concluded that Secamone afzelii hasgreat potential for use as a plantÃ¢â‚¬Âbased biopesticide for controlling C. maculatuson stored cowpea.
Nephrotoxicity as a cause of death in male wistar rats exposed to toxic doses of paracetamol\methionine combination
Authors: Iyanda A. A. , Anetor J. I. , Adeniyi F. A. , Iheakanwa C. I.
Published: August 2021

In an earlier study, we observed that male Wistar rats administered withtoxic doses of methionine containing paracetamol formulation(acetaminophen) did not manifest hepatic necrosis even at doses as high as3000 mg\kg and 5000 mg\kg body weight (BW) yet death occurred. Thisstudy sets out to investigate the cause of death by focusing on another sensitiveorgan to acetaminophen exposure and to highlight the role of some vitaminsin this. Thirty male Wistar rats were divided into six groups consisting 5rats in each, and further administered with different doses of paracetamol\methionine, ranging from 100 mg\kg Ã¢â‚¬â€œ 5000 mg\kg. 5 rats, suppliedwith only physiologic saline were considered as control. Results show thatrats exposed to 100mg\kg, 350 mg\kg and 1000 mg\kg BW did not exhibitany form of renal abnormality. The nephrotoxic indices consisting of urea,creatinine and uric acid were not significantly increased in comparison tocontrol (p>0.05). Renal histology was also not identified as abnormal; moreover0% mortality was recorded for these groups. However, the creatininewas significantly increased in 3000 mg\kg group (p
Induction of Colon tumor and Intestinal tumor in Swiss Albino Mice by the DihydroMicrocystin Ã¢â‚¬â€œLR, [DÃ¢â‚¬ÂAsp3] MicrocystinÃ¢â‚¬ÂWR and [DÃ¢â‚¬ÂAsp3] MicrocystinÃ¢â‚¬ÂHtyR produced by the Cyanobacterial strains Isolated from water bodies in semiÃ¢â‚¬Âdesert region of India
Authors: Kumar R. Ashwin , Ramani K. , Verma S. K.
Published: August 2021

The crude extracts of two cyanobacterial strains, isolated from water bodiesof Shekhawati region of India, and identified as Anabaena doliolum andNostoc spongiaeforme,  inÃ¢â‚¬Âvivo mouse bioassay model. A dose of 100μg of totalprotein/kg of body weight showed restlessness, spasmodic leaping, slowmovement and loss of co Ã¢â‚¬Âordination within 6hours of intraperitonial (i.p)administration in Swiss albino mice. Further purification of crude extractswith the help of thin layer chromatography using silica gel GF 60 plates,showed four spots for each of these strains. The chronic toxicity studies withsub lethal dose of spot Ã¢â‚¬Â3 from A. doliolum have shown to cause colon tumors,whereas sub lethal dose of spot Ã¢â‚¬Â3 from N. spongiaeforme induced theintestinal tumors. An increase in oxidative stress (MDA level), serum ALT,ALP, AST and LDH level was also found in both the cases. These spots werefurther analysis with HPLC and MALDI TOFF Ms and were identified as DihydroMicrocystin –LR and [D Ã¢â‚¬ÂAsp3] MicrocystinÃ¢â‚¬ÂWR in case of Ad spotÃ¢â‚¬Â3 andMicrocystin Ã¢â‚¬ÂLR and [DÃ¢â‚¬ÂAsp3] microcystinÃ¢â‚¬ÂHtyR in case of Ns spotÃ¢â‚¬Â3.showed high level of hepatotoxicity and tumor promotingactivity in
Enzymeâ€assisted extraction of essential oils from Syzygium aromaticum
Authors: Amudan R. , Kamat D. V. , Kamat S. D.
Published: August 2021

The essential oil of clove ( Syzygium aromaticum) holds an important positionamongst widely used essential oils. A typical steam distillation processfor the extraction of clove oil provides a 10.1% yield. Recent studies involvedthe use of enzymes such as pectinase, amylase, lignocellulase, and cellulaseon the powder of clove buds, prior to extraction. The traditional methods ofphysical and chemical extraction are effective but may affect the structure,quality and yield of the phytochemicals extracted. In the current study,hence, enzymes specific for action on the cell wall have been used in the pretreatment prior to extraction, to enhance the quality and yield of the phytochemicalsextracted. The results indicated that all the enzymes, gave morethan 50% higher yield than control in terms of weight of extracted essentialoil. A mixture of the enzymes gave the highest yield of 17.82%. Gas chromatographyresults indicated that the essential oil extracted using amylase hada maximum eugenol content of 70%, in comparison with the eugenol content(62–68%) in the essential oils extracted using the rest of the enzymes.Antibacterial activity of all the extracts was studied on methicillin â€resistantStaphylococcus aureus  (MRSA). The essential oil extracted by using amylaseinhibitedMRSA, showed a zone size of 40 mm, whereas the essential oil extractedby using lignocellulase showed a zone size of 45 mm. The gas chromatogramindicated the maximum number of peaks in this extract, whichcould be producing a combined antibacterial effect on the organism. Thespecific gravity values of the essential oil extracted using lignocellulase andamylase was 1.051 and 1.062, respectively, whereas the control had a specificgravity of 1.015.
Effect of Auxin (IBA) in Sand and Coco peat media on rooting of Hibiscus (Hibiscus rosaâ€ sinensis â€˜yellow double hybridâ€™)
Authors: Shadparvar V. , Torkashvand A. Mohammadi , Alamshiri H. Alinejad
Published: August 2021

Hibiscus rosaâ€ sinensis  â€ sinensis â€ sinensis â€1) in sand and coco peat mediawere used for evaluation of plant rooting in an experiment with completelyrandomized design in three replications. The cuttings were selected whenthey had 6 â€8 buds and their heights were 12â€10 cm. After sterilization andhormonal treatment, cuttings were planted in 3 cm depth of media. In thisstudy, factors such as callus time, root initiation, number of buds, percentageof rooting, number of roots and root length were determined. Theresults showed that with increase in levels of rooting hormones, callusinduction time and time of root beginning was reduced. On the other hand,percentage of green cuttings without root, percentage of rooting and numberof root in cuttings and length of roots produced in each cutting was increased.According to the measured factors, treatment of 2000 mg l â€1 wasbetter treatment in increasing percentage of rooting and other affecting factorson the quality of cuttings in both medium.‘yellow double hybrid’ is a hard rooting plant. Fourlevels of IBA (0, 1000, 2000 and 4000 mg l
Bioautography and antifusaric activity of three tar extracts from the pyrolysis of three plants growing wild in the south-west of Algeria
Authors: Ali Boulanouar  , Benlarbi Larbi  , Akermi Moulay M’hammed
Published: August 2021

Vegetable tars are newly formed resinous products extracted by pyrolysis from wood or bark. Since prehistoric times, they have been used for their adhesive, water-repellent, repellent and finally medicinal properties. This work investigated the use of these vegetable tars as sources of bio-fungicides. The physicochemical properties of the tars obtained in the labor-atories showed a significant yield of 1.22%, 0.66% and 0.51% respectively for the wood of (Jeniperus phoenicea, Olea europeae sylvestris and Acacia torti-lis Sub raddiana). They also showed a density value of 1.22 and a refractive index of about 1.5115 for J.phoenicea, an acid number of 0.39 and a dry matter ratio of 19.86% for A. tortilis Sub raddiana. Considerable antifungal activity of the acetate of three tars against certain fungal phytopathogens (Fusarium) was observed with the most remarkable MIC values ranging from 0.1 mg / ml to 0.46 mg / ml. The J. phoenicea extracts were more active against F. oxysporum f sp albedinis (FOA) strains. The growth of F. solani was more sensitive to acetate extracts from O. europeae. Dichloromethane ex-tracts of A. tortilis Sub raddiana yielded with an average MIC of 0.16 and 1.02 mg / ml, respectively for strains of F. oxysporum and F. graminearum. The bioautography test revealed the presence of 3 antifusaric molecules of interest. These MIC results further merit in vivo tests for the development of future biofungicides.
Assessing the exposure of Sidi M’Hamed Benali lake (Algeria) to Organo-chlorinated compounds and pesticides in fish
Authors: Naima El Badaoui  , Evnor Steve Lutra Mavoungou Taty  , Youcef Amar  , Celia Joaquim-Justo
Published: August 2021

Sidi M’Hamed Benali lake (Algeria) is a reservoir used for recreational and fishing purposes that also harbors a rich fauna an flora. These are affected by use of pesticides in view of the increasing anthropogenic activities. In this study, levels of contaminants, polychlorinated biphenyls (PCBs), polybromo-diphényléthers (PBDEs), dichlorodiphenyltrichloroethanes (DDTs), and Lin-dane were measured using gas chromatography with electron capture detec-tion and gas chromatography mass spectrometry in four fish species (Cyprinus carpio, Barbus barbus, Hypophthalmichthys molitrix, Rutilus rutilus) of Sidi M'Hamed Benali lake. The results showed that PCBs, PBDEs and the insecticide Lindane contaminated the studied species but at concentrations, much lower that the guiding values. On the contrary, high levels of pp’DDE (p,p’dichlorodiphényldichloroéthylène) was recorded in all samples with values higher than the Maximum residue limit of 300 ng/g lipid weight (LW.) set by FAO with values as high as 3.87 x 103 ng/g LW (total DDTs) which indi-cated potential hazard not only to human consumers of the lake fishs but also a potential hazard expected to animals that prey on these fishes. 14 ng/g fresh weight (FW.) Canadian limit is also significantly lower than the values of fish from the studied lake (42-46 ng/g FW.). In light of these results, we conclude that this lake is exposed to organic pollution of anthropogenic origin and we encourage caution regarding the consumption of fish in this reservoir. It is time to make serious efforts to mitigate the lake pollution in order to protect aquatic wildlife for sustainable development.
Endoxylanase production from the solid state fermentation of Mustard pod husk by Penicillium janthinellum
Authors: Kundu Aditi , Ray Rina Rani
Published: August 2021

With an aim to dispose huge amount of mustard pod husk generated after oil seed extraction in an economical way, it was used as the sole carbon source in a fed batch culture of a locally isolated strain of Penicillium janthinellum for the production of endoxylanase (EC 3.2.1.8) on solidâ€state fermentation. The influence of mustard pod husk concentration, cultivation temperature, initial pH, moisture content, organic and inorganic nitrogen sources, surfactants and metal ions were evaluated with respect to endoxylanase production. Attempts were also made to increase the enzyme production by acid and alkali pretreatment of the husk. The hyperproductive strain showed highest endoxylanase production at 72nd h with a temperature and pH optima of 27°C and 6.0 respectively. Inorganic nitrogen sources were proved to have better enhancing effect for enzyme production than the organic sources. Mild alkali treatment made the husk more accessible for the fungal degradation. Ultrafiltered enzyme showed a temperature and pH optima 60°C and 6 respectively and about 80% of the enzyme activity was found to be retained at 60°C for 10 min and at a broad pH range of 4â€9.
Screening of promising accessions of urdbean (V. mungo L.) from different agroâ€ecological zones of Southern and Southâ€“Eastern Rajasthan (India) based on biometrical attributes
Authors: Punia S. S. , Ram Baldev , Dheer Meenakshi , Raghuvanshi A. , Koli N. R. , Ranwha B. R. , Khedar O. P. , Maloo S. R.
Published: August 2021

Genetic variability among 133 accessions of urdbean (Vigna mungo) collected from different agroâ€ecological zones of Southern and South–Eastern Rajasthan, India was assessed for several qualitative and quantitative traits. These accessions along with two national checks KU 96â€3 and RBU 38 were evaluated in randomized block design with three replications at experimental field of Agricultural Research Station, Ummedganj, Kota (25.18°N and 75.86°E), Rajasthan, India during kharif 2009 and 2010. Wide range of variability was observed for both qualitative and quantitative traits. Plant height (cm), primary branches and pods per plant showed high genetic advance and moderate to high heritability. This suggests that the selection based on these traits would be useful in improving the seed yield. The genotypes like KPU 1099, KPU 1057 and KPU 1086 can be used as promising donor parents in recombination breeding programme for obtaining high heterotic response and better segregants.
Effect of electromagnetic field on germination of groundnut seeds (Arachis hypogaea L)
Authors: Rajasekhar E. , Kumar R. Jeevan , Subhan C. M. , Panduranga P. , Krishnamurthy T.
Published: August 2021

Present work is about the influence of Electromagnetic field [EMF] treatment on the improvement of groundnut seeds (Arachis hypogaea L) germination. The treatment consisted of different electromagnetic field strengths 2, 4, 7 and 10 milli Tesla [mT] in different exposure times 10, 20, 30 and 40 min. In every measurement, the relative humidity and room temperature were recorded. The germination [G] of seed in terms of percentage [% ], the stems length [SL] and roots length [RL] in millimeter [mm] at 6th day and 12th day after experiment, and the total weight [TW] in milligram at 12th day have been measured. Best results have been obtained for variants with exposure time of 30 min and field strength of 7 mT at south pole. Result obtained in the present investigation revealed that the energy absorbed by molecules was high at lower output strength and shorter exposure time improved biologicalfunctions, stimulation effect could be achieved.
Micropropagation of Rosa damascena Mill. through transverse thin cell layer culture
Authors: Kshirsagar Kavita , Braganza V. J.
Published: August 2021

A basic factor underlying the success of largeâ€scale micropropagation and genetic transformation of any plant species is regeneration. In order to regenerate propagules of Rosa damascena Mill. on a large scale, an efficient and improved in vitro propagation system has been established using transverse thin cell layer culture (tTCL). By optimizing the position of the tissue and applying an improved selection procedure, in vitro shoots were elongated in 8 weeks of culture. Modified Murashige and Skoog (1962)(MS) medium fortified with 4.0 mg lâ€1 6â€benzylaminopurine (BAP) and 0.4mg lâ€1 anaphthalene acetic acid (NAA) gave optimal shoot regeneration. The explant was inoculated on this medium in the upright position and exhibited a high frequency of shoot regeneration (~96.66%), and it also gave the highest number of shoots (22.33/explant). The horizontally placed explant on an average 7.66 shoots/explant. Our experiments indicate that explant orientation strongly influences the organogenesis response. The frequency of shoot initiation and the number of multiple shoots produced from each explant were significantly dependent on the plant source, concentration of plant growth regulators and the orientation of the explants and contributed significantly to in vitro regeneration. Rooting of well developed shoots was achieved on hormone free ¼ strength MS medium with 4% sucrose.
Temporal variation in free proline content in some species of family Acanthaceae in the southern Rajasthan, India
Authors: Meena K. L.
Published: August 2021

Proline is considered to be associated with stress tolerance in plants. In the present study, free proline was estimated in the root, stem and leaves of 12 species of family acanthaceae collected in different seasons during 2011 from southern Rajasthan. It is observed that the proline content was higher in summer seasons in comparison to other seasons. The maximum content of proline was reported in the stem of Elytraria acaulis and minimum in roots of Justicia quinqueangularis.
Seroprevalence of cytomegalovirus among antenatal patients attending primary health centres in some parts of Kaduna State, Nigeria
Authors: Kolo R. L. , Umoh V. J. , Jatau E. D. , Ella E. E.
Published: August 2021

Cytomegalovirus (CMV) can cross the placenta and cause both fetal and placental infections a serious risk factor for the unborn child. CMVâ€infected infants can be symptomatic and asymptomatic at birth, with outcomes that can include intrauterine growth restriction, hydrocephalus, microcephaly, intracranial calcifications, hearing loss, hepatomegaly, splenomegaly, thrombocytopenic and various degrees of sensory impairment. This work was aimed at determining the seroprevalence of CMV and the risk factors associated with the infection in  antenatal patients attending Primary Health Centersin some part of Kaduna State, Nigeria. Serological screening for CMV antibodies was done using Enzyme Linked ImmunoSorbent Assay (ELISA) IgG and IgM. Of the 360 patient samples, 357 (99.2%) and sixty six (66) (18.3%) were positive for both IgG and IgM respectively. Prevalence of CMV IgG increased with age with 100% prevalence in age groups of greater than 29 years, while that of IgM was higher in ages less than 20 and greater than 40 years (21.3% and 50% respectively). The prevalence of CMV IgG was highest (100%) among those with informal and tertiary education women while the IgM prevalence was highest in those with tertiary education (22.7%) only. In relation to progression of pregnancy, a prevalence of 100% for CMV IgG was observed in first trimester and 22.1% in third trimester for CMV IgM. There was a significant association in CMV IgM seroprevalence (p<0.05) with marital status with the separated having higher prevalence than the married women, but no significant association between CMV and age, socio demographic factors, risk factors and clinical data (P>0.05).
Genetic diversity of Callosobruchus maculatus Fabricus (Cowpea weevil) populations in various agroâ€ecological areas of five countries in West African subâ€region
Authors: Tine EugÃ¨ne Mbar , Diome ToffÃ¨ne , Khadim Kebe , Ndong Assane , Douma Ali , Ketoh Guillaume , Sanon Antoine , Sembene MbackÃ©
Published: August 2021

The cowpea (Vigna unguiculata (L) Walp) is a legume of African origin. It is source of very important nutritional needs like protein and vitamins for the developing countries especially countries of West Africa. The attack by the Bruchinae seedâ€beetle Callosobruchus maculatus (Fab) whose larvae develop in seeds causes losses ranging from 30 to over 80% of the harvest between 6 â€7months of storage. The objectives of this study were to identify the different haplotypes of the weevils’ West African subâ€region, to study genetic diversity in different agroâ€ecological zones and then highlight the phylogenetic affinities between the weevils from these different areas. In this study, we have analysed the sequences of the mitochondrial gene cytochrome b and 28S ribosomal gene in 75 individuals. 42 haplotypes for cytochrome b have been identified in three clades against 30 for the 28S divided into two clades. Individual haplotypes were mainly from the Guinean zone. Genetic distance and nucleotide diversity showed a trend of population structure of weevils between the different agroâ€ecological zones. This work is corroborated by low values of diversity within the zones and highly significant between them. Although Sudanian’ zone seemed not to confirm this view because of a relatively small percentage of alignment for individuals from Tenkodogo (Burkina Faso). The phylogenetic reconstructions have thus shown that gene flow was maintained even if it remained within narrow zones between agroâ€ecological zones for the populations of West African C. maculatus.
Pattern of using pesticides against pests of halieutic products in the Thies region, department of Mbour, Senegal
Authors: Mbaye Ndeye Ndiabe , Sarr Makhfousse , Tine Emmanuel , Samb Abdoulaye , Sembene Mbacke
Published: August 2021

This preliminary investigation is part of the work which demonstrates the pattern of pesticide use in the preservation of fish product in Senegal, particularly in the sites of the small coast which is the most important. We did our research in three sites of Department of Mbour, where actors which work in the fisheries product processing use synthetic pesticides, for elimination and/or prevention of any insect infestation. The product Actellic (used by 12.50% of our respondents) which is a mixture of pirimiphosâ€methyl and permethrin and another insecticide called Kaata (used by 37.50% of our respondents) of unknown composition, are used by workers without metering or protective measures in handling. Among the participants of this study, 14% were found to be affected by the use of these chemicals. Such pattern of indiscriminate use of pesticides without following safety guidelines poses the risk of chronic intoxication to the workers engaged in trading of fish products in the study area, and can have longâ€term adverse consequences, especially for children.
Evaluating antimutagenic effects of vitaminâ€C and Lâ€cysteine alone and in combination towards sodium azideâ€induced toxicity in Trigonella foenumâ€ graecum L.
Authors: Rawat Manoj Kumar , Meena Rajendra Prasad
Published: August 2021

Antimutagenicity of vitaminâ€C (ascorbic acid) and Lâ€cysteine (amino acid) was determined against the mutagenicity of sodium azide (NaN3; pH 6.0) in Trigonella foenumâ€graecum L. Toxicity to seed germination and seedling growth were taken as as the parameters. Sodium azide (mutagenic agent) induces inhibitory effects on the seed germination and causes formation of abnormal seedlings significantly with increasing concentrations. Sodium azide mutagenized seeds of T. foenumâ€graecum postâ€treated with four different aqueous concentrations of vitaminâ€C (0.0005M, 0.0010M, 0.0015M and 0.0100M) and Lâ€cysteine (0.0005M, 0.0010M, 0.0015M and 0.0100M), exhibited antimutagenic activity by way of promotory effects on seed germination as well as on seedling growth as compared to contol. Lâ€cystein was found to be more potent as antimutagen (antitoxic) than vitaminâ€C.
Antibiotic Susceptibility Profiles of Escherichia coli from Urinary Bacteruria of Asymptomatic Students of University of Ibadan
Authors: L Okunye Olufemi , Rukayatt Sadiq , S Ayedun Joshua
Published: August 2021

Asymptomatic bacteruria is the presence of significant count bacteria capable of inducing urinary tract infection in the urine tract of clinically healthy individuals. Four hundred samples of urine were investigated for the presence of Escherichia coli in this study, out of which forty by conventional biochemical tests. The percentage prevalence gender ratio of the isolates was 77.5% females to 22.5% males while the age distribution prevalent ranged between 24 years 30years. Ten conventional biochemical confirmatory tests was carried out on the isolates. The antibiogram in this study elicited the effective potency of floroquinolones while ampicillin, a β –lactams derivatives was recorded to be less effective. Sixteen isolates showed multidrug resistant to 4 antibiotics while 2 isolates elicited multidrug resistance to 6 antibiotics in this study. The findings of this study the prevalence and the potency of selected antibiotics could be useful in the therapeutic management of asymptomatic bacteruria in the study region.
Comparative evaluation of crop productivity and profitability under traditional farming and integrated farming system in Northern plains of India
Authors: Rai R. B. , Dhama K. , Chakraborty S. , Ram R. A. , Singh Balvir , Tiwari R. , Saminathan M. , Damodaran T.
Published: August 2021

Agriculture is the mainstream of livelihood security in northern plains of India, however, production of all crops is below expected levels. Various studies have indicated that impact of recent technological innovations is not up to desired levels. The present study was conducted to evaluate profitability of various models in existing and intervened integrated farming system (IFS) along with acceptance of farmers in northern plains of Uttar Pradesh, India. Twenty farmers from each district viz. Barabanki and Raebareli, were monitored with all accessible records. Various interventions in their farming system were made which included new technology of rural poultry production (Modelâ€1), infertility control, vegetable production using our bioâ€enhancer (CSRâ€BIO), gladiolus, banana (Gâ€9 variety), guava (semiâ€intensive), goat and awareness about improved composting and quality seeds. The preâ€dominant farming system with 16 families was paddyâ€wheatâ€fallow with benefit: cost (B: C) ratio of 1.83. This was followed by paddyâ€wheatâ€green gramâ€bovines in 7 families with B: C ratio of 1.66. The highest B: C ratio of 4.78 was observed in modelâ€R i.e. vegetables cultivations on river bank due to varietal and CSRBIO interventions. The lowest B: C ratio (2.09) in post intervention period was observed in modelâ€O (guavaâ€bovineâ€vegetables) due to no return from guava in first 2 years. The family annual income in the survey increased significantly. The IFS models, if introduced, are highly useful and uniform acceptance of the components takes time. From the present study, it clearly emerged that IFS gives higher B: C ratio than the existing cropping/ farming system.
Impact of gaseous air pollutants on lipid and protein content of beans (Phaseolus vulgaris L.) plant growing at urban, subâ€urban, and rural sites of the Riyadh, Kingdom of Saudi Arabia
Authors: Almuwayhi Mohammed AbdulRahman
Published: August 2021

The current study was conducted at three locations varying in pollution with ozone, sulfur dioxide and nitrogen dioxide in the Riyadh, Kingdom of Saudi Arabia. Phaseouls vulgaris was sown in the pots and irrigated with different concentrations of ascorbic acid solution and it was grown in polluted areas. The results showed that the concentrations of polluting gases in the air in the Riyadh increased during the study period, reaching the concentration of ozone gas to 77 ppb in urban area, and sulfur dioxide to 21 ppb in the suburban area, and nitrogen dioxide to 22 ppb in the urban area. There was a significant effect of those pollutants on the protein and fat content of the test plant, where the content of protein in the leaves was 4.09% at the rural area, 3.87% in the suburban area and 3.81% at the urban area. At the same time the lipid content of the leaves was 2.7% at rural area, 2.9% in the subâ€urban area and 2.3% in the urban area. The treatment of ascorbic acid increased the level of protein and lipid contents of these plants. Protein content in the leaves was 4.73% at rural area, 4 % in the subâ€urban area and 3.97% in the urban area. The lipid content of the leaves was 3 % at rural area, 2.7% in the subâ€urban area and 2.6% at the urban area. This study proved the role of ascorbic acid (ASA) in the plant as it increased the resistance against the ozone gas oxidizer, and thereby helping in the removal of toxic reactive oxygen species.
Cytological assays for the evaluation of genotoxicity in plants: A review
Authors: Siddiqui Sazada
Published: August 2021

Plants model are recognized as excellent genetic models to detect environ-mental mutagens and are frequently used in xenomoitoring studies. Several assays such as chromosomal aberration assay, micronucleus assay and comet assay are used for detecting the mutagenicity, cytotoxicity and genotoxicity of environmental pollutants. It is easily handled and has advantages over other short-term tests that require pervious preparation of tested samples as well as the addition of exogenous metabolic system. Plants model also enables to evaluate different end points such as chromosomal aberration assay (CAA), micronucleus assay (MNA) and comet assay (CA) that have been used to detect genotoxicity of environmental pollutants. In addition, plants model provides important information to evaluate action mechanisms of an agent about its effects on the genetic material (clastogenic and/or aneugenic effects). It has been widely used to assess the impacts caused by xenobiotic, characterizing an important tool for environmental monitoring studies. The present review describes the two important assays using plant models that are appropriate and efficient cytogenetic materials for the detection of geno-toxicity of environmental pollutants.
Development, Optimization and Validation of a Sandwich ELISA for Detection of Recombinant Human Factor VII
Authors: Sarial Sheila , Sonboli Rozhan , Maleknia Shayan , Ashori Fatemeh , Rezaeiravesh Sara , Nouri S. Zomorrod , Abolhassan Mohammadreza , Vaziri Behrouz , Mahboudi Fereidoun
Published: August 2021

Recombinant human factor VII (rh-FVII) is produced by engineered BHK cells at low dose. Accordingly, establishment of a precise method is crucial to reliably measuring expression level of this protein during manufacturing pro-cesses. We developed and established a reproducible sandwich enzyme-linked immunosorbent assay (ELISA) method for measuring amount of FVII during biopharmaceutical in upstream and downstream processes of Aryo-Seven. A sandwich ELISA was designed using two different high affinity mon-oclonal antibodies (mAb1 and mAb2) against h-FVII. The bounded FVII to the first antibody was revealed by the use of a second mouse anti-FVII monoclo-nal antibody (1F1-B11), labeled with HRP that binds to another antigenic determinant of the FVII. Then, validation was done by determination of spec-ificity, linearity, accuracy, precision, reproducibility, detection and quantifica-tion limit and robustness according to ICH Q2 (R1) guideline. In developed ELISA, no interference was found between FVII and proteins derived from BHK which commonly exist in the supernatant. Linear range of detection was from 25-1.56 ng/mL with P-Value <0.001. In accuracy, spiked samples showed 109±2% recovery. Intra and intermediate precision assays showed %RSD not more than20. Detection limit of this assay was 0.99 ng /mL and limit of quantification was 2.99 ng/ml. The sandwich ELISA was found to be useful tool for measuring FVII/ FVIIa. The ELISA approach was precise, repro-ducible, and accurate. The ELISA therefore, is offered as an assured kit for detection of recombinant human factor.