bluuebunny/crossref_metadata_2025 huggingface dataset:
Custom selected reference genes outperform pre-defined reference genes in transcriptomic analysis
Authors: Santos Karen Cristine Gonçalves Dos , Desgagné-Penix Isabel , Germain Hugo
Published: October 2019

Background: RNA sequencing allows the measuring of gene expression at a resolution unmet by expression arrays or RT-qPCR. It is however necessary to normalize sequencing data by library size, transcript size and composition, among other factors, before comparing expression levels. The use of internal control genes or spike-ins is advocated in the literature for scaling read counts, but the methods for choosing reference genes are mostly targeted at RT-qPCR studies and require a set of pre-selected candidate controls or pre-selected target genes.
Results: Here, we report an R-based script to select internal control genes based solely on read counts and gene sizes. This novel method first normalizes the read counts to Transcripts per Million (TPM) and then excludes weakly expressed genes using the DAFS script to calculate the cut-off. It then selects as references the genes with lowest TPM covariance. We used this method to pick custom reference genes for the differential expression analysis of three transcriptome sets from transgenic Arabidopsis plants expressing heterologous fungal effector proteins tagged with GFP (using GFP alone as the control). The custom reference genes showed lower covariance and fold change as well as a broader range of expression levels than commonly used reference genes. When analyzed with NormFinder, both typical and custom reference genes were considered suitable internal controls, but the expression of custom selected genes was more stable. geNorm produced a similar result in which most custom selected genes ranked higher (i.e. expression more stable) than commonly used reference genes.
Conclusions: The proposed method is innovative, rapid and simple. Since it does not depend on genome annotation, it can be used with any organism, and does not require pre-selected reference candidates or target genes that are not always available.
Escherichia Coli O157:H7: Distribution, molecular characterization, antimicrobial resistance patterns and source of contamination of sheep and goat carcasses at an export Abattoir, Mojdo, Ethiopia
Authors: Abreham Solomon , Teklu Akafete , Cox Eric , Tessema Tesfaye Sisay
Published: October 2019

Background : Cattle have been identified as a major reservoir of E. coli O157:H7 for human infection; the ecology of the organism in sheep and goats is less understood. This study was carried out to determine prevalence, source of infection, antibiotic resistance and molecular characterization of Escherichia coli O157: H7 isolated from sheep and goat.
Methods : Systematic random sampling was carried out at Modjo export abattoir, Ethiopia, from November 2012 to April 2013 to collect 408 samples from 72 sheep and 32 goats. Samples collected were skin swabs, fecal samples, intestinal mucosal swabs and the inside and outside part of carcasses as well as carcass in contacts such as workers hands, knife, hook and carcass washing water. Then, samples were processed following standard bacteriological procedures. Non-Sorbitol fermenting colonies were tested on latex agglutination test and the positives are subjected to PCR for detection of attaching and effacing genes ( eaeA) and shiga toxin producing genes ( stx1 and stx2 ). All E. coli O157:H7 isolates were checked for their susceptibility pattern towards 15 selected antibiotics.
Results : E. coli O157:H7 were detected in only 20/408 samples (4.9%). Among these 20 positive samples, 70% (14/20), 25% (5/20) and 5% (1/20) were from sheep, goats and knife samples, respectively. No significant associations were found between carcasses and the assumed sources of contaminations. Of all the 20 isolates virulence genes were found in 10 (50%) of them; 3 (15%) with only the eaeA gene and 7(35%) expressing eaeA and stx2 genes. All the isolates were susceptible to Norfloxacin (NOR) (100%).
Conclusions : The presence of virulence genes shows E. coli O157:H7 is a potential source of human infection in Ethiopia.
Key words : Abattoir, antibiotic sensitivity, CT-SMAC, E. coli O157:H7, IMS, Latex agglutination, multiplex PCR.
Cognitive impairments in patients with persistent symptoms attributed to Lyme disease
Authors: Berende Anneleen , Rentergem Joost Agelink van , Evers Andrea W. M. , Hofstede Hadewych J. M. ter , Vos Fidel J. , Kullberg Bart Jan , Kessels Roy P.C.
Published: October 2019

Background: Persistent symptoms attributed to Lyme borreliosis often include self-reported cognitive impairment. However, it remains unclear whether these symptoms can be substantiated by objective cognitive testing. Methods: For this observational study, cognitive performance was assessed in 280 adults with persistent symptoms attributed to Lyme borreliosis (as part of baseline data collected for the Dutch PLEASE study). Cognitive testing covered the five major domains: episodic memory, working memory/attention, verbal fluency, information-processing speed and executive function. Patients’ profiles of test scores were compared to a large age-, education- and sex-adjusted normative sample using multivariate normative comparison. Performance validity was assessed to detect suboptimal effort, and questionnaires were administered to measure self-reported cognitive complaints, fatigue, anxiety, depressive symptoms and several other psychological factors. Results: Of 280 patients, one was excluded as the test battery could not be completed. Of the remaining 279 patients, 239 (85.4%) displayed sufficient performance validity. Patients with insufficient performance validity felt significantly more helpless and physically fatigued, and less orientated. Furthermore, they had a lower education level and less often paid work. Of the total study cohort 5.7% (n=16) performed in the impaired range. Among the 239 patients who displayed sufficient performance validity, 2.9% (n=7) were classified as cognitively impaired. No association between subjective cognitive symptoms and objective impairment was found. Conclusions: Only a small percentage of patients with borreliosis-attributed persistent symptoms have objective cognitive impairment. Performance validity should be taken into account in neuropsychological examinations of these patients. Self-report questionnaires are insufficiently valid to diagnose cognitive impairment.
Rates and Predictors of Switching to Tenofovir alafenamide-containing ART in a nationwide cohort
Authors: Surial Bernard , Cavassini Matthias , Calmy Alexandra , Fehr Jan , Stöckle Marcel , Bernasconi Enos , Roth Bianca , Fux Christoph A. , Kovari Helen , Furrer Hansjakob , Rauch Andri , Wandeler Gilles
Published: October 2019

Background
Tenofovir alafenamide (TAF)-containing combinations were introduced in Switzerland after October 2016 and are recommended over tenofovir disoproxil fumarate (TDF) in patients with osteoporosis or impaired renal function.
Methods
We included all participants of the Swiss HIV Cohort Study on TDF-containing antiretroviral therapy with follow-up visits after January 2016. We determined the proportion of switches from TDF to TAF overall, and among patients with risk factors for TDF toxicity, including osteoporosis, impaired renal function or marked proteinuria. We used multivariable logistic regression to explore predictors of switching from TDF to TAF.
Results
We included 5’012 patients, of whom 652 (13.0%) had risk factors for TDF toxicity. A switch from TDF to TAF was undertaken in 2’796 (55.8%) individuals overall, and in 465 (71.3%) with risk factors. Predictors of switching to TAF were male sex (adjusted odds ratio 1.27, 95% confidence interval 1.07-1.50), age >50 years (1.43, 1.23-1.66) and the presence of risk factors for TDF toxicity (2.21, 1.77-2.75). In contrast, patients with non-nucleoside reverse transcriptase inhibitor (NNRTI)-based single-pill regimen (0.11, 0.09-0.13), those treated in non-tertiary centers (0.56, 0.46-0.70), as well as those with CD4 cell counts below 500/µL (0.77, 0.66-0.90) and with chronic hepatitis C infection (0.66, 0.54-0.80) were most likely to stay on TDF.
Conclusions
Over 50% of patients on TDF-containing therapy, including the majority of patients at risk for TDF toxicity, were switched to TAF within two years of its introduction in Switzerland. Individuals on NNRTI-based single-pill regimens were most likely to remain on TDF.
Bioinformatics and DNA-extraction strategies to reliably detect genetic variants from FFPE breast tissue samples
Authors: Bhagwate Aditya Vijay , Liu Yuanhang , Winham Stacey J. , McDonough Samantha J. , Stallings-Mann Melody L. , Heinzen Ethan P. , Davila Jaime I. , Vierkant Robert A. , Hoskin Tanya L. , Frost Marlene , Carter Jodi M. , Radisky Derek C. , Cunningham Julie M. , Degnim Amy , Wang Chen
Published: October 2019

Background: Archived formalin fixed paraffin embedded (FFPE) samples are valuable clinical resources to examine clinically relevant morphology features and also to study genetic changes. However, DNA quality and quantity of FFPE samples are sub-optimal, and resulting NGS-based genetics variant detections are prone to false positives. Evaluations of wet-lab and bioinformatics approaches are needed to optimize variant detection from FFPE samples.
Results: As a pilot study, we designed within-subject triplicate samples of DNA derived from paired FFPE and fresh frozen breast tissues to highlight FFPE-specific artifacts. For FFPE samples, we tested two FFPE DNA extraction methods to determine impact of wet-lab procedures on variant calling: QIAGEN QIAamp DNA Mini Kit ("QA"), and QIAGEN GeneRead DNA FFPE Kit ("QGR"). We also used negative-control (NA12891) and positive control samples (Horizon Discovery Reference Standard FFPE). All DNA sample libraries were prepared for NGS according to the QIAseq Human Breast Cancer Targeted DNA Panel protocol and sequenced on the HiSeq 4000. Variant calling and filtering were performed using QIAGEN Gene Globe Data Portal. Detailed variant concordance comparisons and mutational signature analysis were performed to investigate effects of FFPE samples compared to paired fresh frozen samples, along with different DNA extraction methods.
In this study, we found that five times or more variants were called with FFPE samples, compared to their paired fresh-frozen tissue samples even after applying molecular barcoding error-correction and default bioinformatics filtering recommended by the vendor. We also found that QGR as an optimized FFPE-DNA extraction approach leads to much fewer discordant variants between paired fresh frozen and FFPE samples. Approximately 92 % of the uniquely called FFPE variants were of low allelic frequency range (<5%), and collectively shared a “C>T|G>A” mutational signature known to be representative of FFPE artifacts resulting from cytosine deamination. Based on control samples and FFPE-frozen replicates, we derived an effective filtering strategy with associated empirical false-discovery estimates.
Conclusions: Through this study, we demonstrated feasibility of calling and filtering genetic variants from FFPE tissue samples using a combined strategy with molecular barcodes, optimized DNA extraction, and bioinformatics methods incorporating genomics context such as mutational signature and variant allelic frequency.
Comparison of the effect of three different protein content enteral diets on serum levels of proteins, nitrogen balance and energy expenditure in critically ill infants: study protocol for a randomized controlled trial
Authors: Montes Reyes Fernández , Villaescusa Javier Urbano , Álvarez Ángel Carrillo , Allende Ana Vivanco , García María José Solana , Galán Corsino Rey , Cid Jesús López-Herce
Published: October 2019

Background Nutritional support is essential in the care of critically ill children, since malnutrition in this population is associated to increased morbidity and mortality. Injury in patients admitted to pediatric intensive care units (PICU) results in a catabolic state, with augmented protein breakdown, leading to a negative protein balance. Current recommendations about protein prescription in PICU are fundamentally based on expert opinions, with a minimum threshold of 1.5 g/kg/day of protein, although protein needs could be higher in certain subgroups of patients. The main objective of the present study is to examine if the administration of a protein-enriched infant formula increases the serum levels of total proteins, albumin, prealbumin, transferrin, retinol, and improves nitrogen balance; and to analyze the effect of the high-protein diet on energy expenditure. A secondary objective is to register possible secondary effects of the protein-enriched diet. Methods a multicenter prospective randomized controlled trial (RCT) will be performed in three hospitals. Patients meeting inclusion criteria will be randomly allocated to one of three enteral feeding formula with different protein content. Blood and urine test, nitrogen balance assessment and energy expenditure testing by indirect calorimetry will be performed at the beginning of nutrition regimen and at 24 hours, 72 hours and 5-7 days after initiation. The sample size for this trial is estimated as 90 participants, with approximately 30 participants in each group. The data analysis will be by intention to treat. Discussion this RCT will provide new data about the amount of protein needed to improve levels of serum protein and nitrogen balance, surrogate of protein balance, in critically ill infants receiving enteral nutrition.
Finite element analysis of occlusal splint therapy in patients with bruxism
Authors: Gholampour Seifollah , Gholampour Hanie , Khanmohammadi Hamed
Published: October 2019

Background: Bruxism is among the habits considered generally as contributory factors for temporomandibular joint (TMJ) disorders and its etiology is still controversial.Methods: Three-dimensional models of maxilla and mandible and teeth of 37 patients and 36 control subjects were created using in-vivo image data. The maximum values of stress and deformation were calculated in 21 patients six months after using a splint and compared with those in the initial conditions. Results: The maximum stresses in the jaw bone and head of mandible were respectively 4.4 and 4.1 times higher in patients than in control subjects. Similar values for deformation were 5.8 and 4.9, respectively. The maximum stress in the jaw bone and head of mandible decreased six months after splint application by up to 71.0% and 72.8%, respectively. Similar values for the maximum deformation were 80.7% and 78.7%, respectively. Following the occlusal splint therapy, the approximation of maximum deformation to the relevant values in control subjects was about 2.6 times the approximation of maximum stress to the relevant values in control subjects. The maximum stress and maximum deformation occurred in all cases in the head of the mandible and the splint had the highest effectiveness in jaw bone adjacent to the molar teeth. Conclusions: Splint acts as a stress relaxer and dissipates the extra stresses generated as well as the TMJ deformation and deviations due to bruxism. The splint also makes the bilateral and simultaneous loading possible and helps with the treatment of this disorder through regulation of bruxism by creating a biomechanical equilibrium between the physiological loading and the generated stress.
The value of miR-155 as a biomarker for the diagnosis and prognosis of lung cancer: a systematic review with meta-analysis
Authors: Shao Chuchu , Yang Fengming , Qin Zhiqiang , Jing Xinming , Shu Yongqian , Shen Hua
Published: October 2019

Background: Recently, a growing number of studies have reported the coorelation between miR-155 and the diagnosis and prognosis of lung cancer, but results of these researches were still controversial due to insufficient sample size. Thus, we carried out the systematic review and meta-analysis to figure out whether miR-155 could be a screening tool in the detection and prognosis of lung cancer.
Methods: A meta-analysis of 13 articles with 19 studies was performed by retrieving the PubMed, Embase and Web of Science. We screened all correlated literaters until December 1st, 2018. For the diagnosis analysis of miR-155 in lung cancer, sensitivity(SEN), specificity (SPE), positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR) and area under the ROC curve (AUC) were pooled to evaluate the accuracy of miRNA-155 in the diagnosis of lung cancer. For the prognosis analysis of miR-155 in lung cancer, the pooled HRs and 95% CIs of miR-155 for overall survival/disease free survival/progression-free survival (OS/DFS/PFS) were calculated. In addition, Subgroup and meta-regression analyses were performed to distinguish the potential sources of heterogeneity between studies.
Results: For the diagnostic analysis of miR-155 in lung cancer, the pooled SEN and SPE were 0.82 (95% CI: 0.72-0.88) and 0.78 (95% CI: 0.71-0.84), respectively. Besides, the pooled PLR was 3.75 (95% CI: 2.76-5.10), NLR was 0.23 (95% CI: 0.15-0.37), DOR was 15.99 (95% CI: 8.11-31.52) and AUC was 0.87 (95% CI: 0.84-0.90), indicating a significant value of miR-155 in the lung cancer detection. For the prognostic analysis of miR-155 in lung cancer, up-regulated miRNA-155 expression was not significantly associated with a poor OS (pooled HR = 1.26, 95% CI: 0.66-2.40) or DFS/PFS (pooled HR = 1.28, 95% CI: 0.82-1.97).
Conclusions: The present meta-analysis demonstrated that miR-155 could be a potential biomarker for the detection of lung cancer but not an effective biomarker for predicting the outcomes of lung cancer. Furthermore, more well-designed researches with larger cohorts were warranted to confirm the value of miR-155 for the diagnosis and prognosis of lung cancer.
Sertaconazole in the treatment of vulvovaginal candidiasis
Authors: Efendieva Z. N.
Published: October 2019

Vulvovaginal candidiasis today is one of the most pressing problems in the field of women’s health. However, the prevalence of Candida with formed drug resistance to fluconazole significantly complicates the treatment of this disease. Thus, the evaluation of the safety and efficacy of other antimycotic drugs becomes an important task in the fight against recurrent genital candidiasis. The article presents the data of international and domestic studies on the possibilities of using sertaconazole in the therapy of vulvovaginal candidiasis.
Ursodeoxycholic acid and cancer prevention
Authors: Reyzis A. R.
Published: October 2019

Along with the great progress in the fight against malignant neoplasms through the improvement of surgical care and the development of chemotherapy, the search for strategies and opportunities to prevent cancer is becoming increasingly important and promising. Early detection and rational treatment of chronic precancerous diseases must be a priority. In this regard, the possible role of ursodeoxycholic acid (UDCA, ursosan, etc.) has been closely studied in recent years. An overview of the role of ursodeoxycholic acid (UDCA) in the prevention of cancer is presented. The pathogenetic basis of this action is the proven normalizing effect of UDCA on bile acid metabolism, microbiome condition and apoptosis. The paper summarizes the latest data on the ability of UDCA to withstand malignization in the liver, throughout the gastrointestinal tract and beyond. These numerous experimental and clinical studies allow us to assert that UDCA drugs, except for the well-known and versatile hepatoprotective effect, can be considered as effective and promising means of cancer prevention throughout the digestive tract and associated organs. Further research into the broad spectrum of UDCA’s ability to prevent gastrointestinal malignization and beyond is reasonable and promising.
Potential use of methylmethionine (vitamin U) as a gastroprotector Interview with Prof. Evgeniya V. Shikh, Dr. of Sci. (Med.), Head of Chair for Clinical Pharmacology and Propaedeutics of Internal Diseases, I.M. Sechenov First Moscow State Medical University
Authors: Editorial Article
Published: October 2019

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Tutor Sebaya dalam Upaya Perumbuhan Motivasi dan Minat Baca Siswa
Authors: Purnami Purnami
Published: October 2019

Tujuan penelitian adalah untuk mengetahui pengaruh tutor sebaya terhadap peningkatan motivasi minat baca siswa kelas VI pada SD Negeri Karangtengah IV. Penelitian ini menggunakan pendekatan kualitatif deskriptif. Metode yang digunakan adalah dengan pemanfaatan teman sekelas sebagai tutor sebaya. Hasil penelitian menunjukkan bahwa dalam kegiatan membaca, minat siswa kelas VI SD Negeri Karangtengah VI masih rendah. Namun, ketika berkomunikasi dengan teman sebayanya dalam kegiatan sehari-hari, siswa jauh lebih mudah. Hal tersebut yang mendorong untuk menggunakan teman sebagai tutor sebaya untuk menumbuhkan minat baca. Tahapan pelaksanaan berupa pendahuluan, pelaksanaan, dan kegiatan evalusai. Pemanfaatan tutor sebaya dilakukan ketika kegiatan literasi berlangsung. Siswa yang berperan sebagai tutor sebaya, memandu temannya yang kurang berminat dalam kegiatan membaca. Dalam satu kelas dibentuk menjadi empat kelompok, dengan tiap kelompok memiliki satu tutor sebaya. Kendala yang terjadi di lapangan adalah harus ada kejelian dalam memilih siswa untuk menjadi tutor sebaya. Efektifitas tutor sebaya dalam kegiatan pembelajaran adalah adanya peningkatan minat baca pada siswa dalam tiap-tiap kelompok. Minat baca tumbuh karena adanya motivasi dari masing-masing anggota kelompok. Dalam tiap kelompok tersebut, antara siswa yang satu memotivasi siswa yang lain. Hasil akhir menunjukkan bahwa dengan menggunakan metode tutor sebaya, minat baca di kelas VI pada SD Negeri Karangtengah IV mengalami peningkatan.
Firm-Specific Currency Exposure, Repatriation, and the Market Value of Repatriation Taxes
Authors: Downes Jimmy F. , Mathis Mollie E. , Kutcher Lisa
Published: October 2019

As the U.S. dollar (USD) strengthens relative to foreign currencies, the USD value of foreign subsidiary-to-parent dividends decreases, and the foreign tax credit remains anchored at a blended rate. During periods of USD strength, this asymmetry lowers the effective tax cost of repatriation at the cost of a lower after-tax dividend to the U.S. parent. This paper develops a firm-specific measure of currency exposure and provides evidence that repatriation likelihood increases during periods of firm-specific USD strength. We show that investors place a premium on repatriation costs when the USD strengthens against a firm-specific basket of currencies for repatriating firms. This premium implies that investors value the benefit of a lower effective tax cost of repatriation more than the potential cost of a lower after-tax dividend available to the U.S. parent. These results appear concentrated in firms with high levels of foreign cash and firms susceptible to earnings fixation.
En diskusjon om universitetets tredje rolle
Authors: Karlsen James
Published: October 2019

In the last decades, the discourse about universities and their engagement with actors in their host regions has increased. Concepts such as the third mission and the entrepreneurial university aim to describe the engagement between the university and regional actors as a change in the role of the university. In theory, this is described as a transformation of the university. In practice, this is organised as an add-on of a range of different knowledge-transfer and market-oriented activities, which do not interfere with the core activities of teaching and research. These normative concepts have a significant influence on how universities are developing their regional engagement. In a case study from a university in Norway, University of Agder, the study shows a university that gradually is transforming from an ivory tower model towards more regional engagement. However, the transformation is taking time. At present, the university has a dual strategy for its regional engagement. One part is organised as an add-on activity, while the other is organised within the academic core of teaching. In the latter, students are co-creating knowledge together with regional actors. The study demonstrates that it is possible to organise regional engagement as co-creation of knowledge also within the academic core.
Health and Safety Disclosures Practice in Indonesian Chemical Industries : A Qualitative Content Analysis Study
Authors: Laoli Warman Berkat , Davianti Arthik
Published: October 2019

This study aims to determine the disclosure for occupational health and safety, as presented in GRI Standard-based CSR. A qualitative approach with content analysis was applied in the study. Content analysis is an analysis based on narrative according to certain criteria which classified through keywords and referred to as disclosure groups. The study focused on companies listed in Indonesia, in the chemical sector. The content analysis examined eleven companies. The results showed that based on the disclosures, all companies had implemented health and safety. Further, it also suggested that the disclosures were not necessary for accordance with GRI Standard.
Relaciones talla-peso y factor de condición de la tilapia Orecochromis niloticus en cinco cuerpos de agua del estado de Jalisco, México
Authors: Santoyo Telles Felipe , Mariscal Romero José , Gómez Galindo Carlos , Gutiérrez Pulido Humberto
Published: October 2019

En este trabajo se analizaron las relaciones talla-peso y factor de condición de 3606 individuos de Oreochromis niloticus colectados desde enero hasta diciembre de 2010 en cinco cuerpos de agua: dos lagos endorreicos, conocidos como lagunas de Zapotlán y de Cajititlán, y tres embalses artificiales de capacidades similares, denominados presas Calderón, El Salto y Basilio Vadillo. También se exploraron las relaciones entre las variables morfométricas mencionadas con variables de calidad del hábitat como temperatura, oxígeno, pH, potencial óxido reducción (ORP) y sólidos disueltos totales (TDS). El análisis del estado nutricional de los individuos medido a través del índice factor de condición demuestra que los peces que habitan los lagos de Zapotlán y Cajititlán son significativamente más pequeños y con una condición más pobre que los que se hallan en las presas Calderón y El Salto, con la excepción de B. Vadillo, la cual presenta valores semejantes a los lagos. Los valores bajos del factor de condición en los lagos se asocian con niveles bajos de temperatura y concentración de oxígeno disuelto, valores altos de pH y sólidos disueltos, así como un valor bajo en el potencial de óxido reducción, atribuidos a la descarga de aguas residuales de las áreas urbanas adyacentes, con una carga orgánica que afecta la calidad del hábitat acuático e impacta la condición de los individuos. En contraste, las tilapias de mejor talla-peso y condición se capturaron en las presas de El Salto y Calderón, donde los niveles de oxígeno son más elevados, pH cercano al neutro, menor cantidad de sólidos disueltos y potencial óxido reductor positivo. Se asume que talla, peso y factor de condición (K) de los individuos de Orechromis niloticus varían en función de la calidad del hábitat.
El aprendizaje orientado en proyectos para el desarrollo de habilidades blandas en el nivel medio superior del IPN
Authors: Zepeda Hurtado María Elena , Cardoso Espinosa Edgar Oliver , Cortés Ruiz Jésica Alhelí
Published: October 2019

El objetivo de esta investigación fue desarrollar en estudiantes de nivel medio superior del Instituto Politécnico Nacional (IPN) habilidades blandas mediante la implementación de la metodología del aprendizaje orientado en proyectos. El tipo de estudio que se empleó fue una metodología cuantitativa con un alcance descriptivo. El instrumento que se utilizó para obtener la información fue un cuestionario basado en una escala Likert. La muestra del estudio estuvo constituida por el alumnado inscrito en el Centro de Estudios Científicos y Tecnológicos perteneciente al IPN. La distribución de la muestra con respecto al género fue de 67 % de hombres y 33 % mujeres. Los principales resultados fueron que los participantes reconocieron un desarrollo muy frecuente de sus habilidades blandas en cuatro rubros: interpersonales, toma de decisiones, pensamiento crítico y autocontrol, y habilidades comunicativas.
Evaluación de los resultados de implementación del modelo de tutoría virtual
Authors: Ramírez Hernández Moramay , Figueroa Moreno Gabriela , Téllez Barrientos Omar
Published: October 2019

La tutoría actualmente se ha convertido en un referente importante dentro del modelo de universidades tecnológicas. Esto debido a que se considera una estrategia efectiva para mejorar el proceso de enseñanza-aprendizaje y, por extensión, para disminuir los índices de reprobación y deserción, así como incrementar la eficiencia terminal. En la Universidad Tecnológica de Tecámac (Uttec) el modelo de tutoría virtual se ha desarrollado con el fin de contribuir de manera significativa en el desarrollo de los estudiantes. El objetivo de este estudio fue conocer los resultados de la implementación de dicho modelo, generado mediante estrategias andragógicas y tecnológicas para facilitar la formación profesional en la Ingeniería en Tecnologías de la Información y Comunicación de la Uttec.

En cuanto a los aspectos metodológicos de la investigación, se consideró un enfoque mixto  debido a que se combinó tanto la parte cuantitativa como cualitativa. Además, por el dinamismo del proyecto, se utilizó tanto el esquema inductivo como el deductivo. Por otro lado, el diseño de la investigación fue no experimental bajo un método de investigación-acción. Respecto a los resultados de la evaluación obtenidos del pilotaje realizado, más de 65.57 % de los estudiantes que trabajaron el modelo de tutoría virtual están totalmente de acuerdo con los resultados; 28.72 % ni de acuerdo ni en desacuerdo, y 5.71 % no está de acuerdo. Aunado a ello, se obtuvieron en la parte cualitativa retroalimentaciones importantes para realizar mejoras en el proyecto, con lo cual se podrá elevar el índice de satisfacción.
Defectos de pared abdominal anterior fetal: manejo y protocolo de seguimiento prenatal de Gastroquisis y Onfalocele. Ecuador, 2019.
Authors: Velasco Jácome Pamela , Mercado Andrés , Paez Luis , Yépez Eduardo
Published: October 2019

Los defectos de la pared abdominal son un grupo de malformaciones congénitas poco comunes que presentan alteraciones heterogéneas y comparten una característica en común, que es la herniación o evisceración de uno o más órganos de la cavidad abdominal, debido a un defecto en la formación de la pared abdominal. Existen diversas patologías entre las que se encuentran extrofia de vejiga y extrofia de cloaca y la Pentalogía de Cantrell, sin embargo, las más frecuentes son la gastrosquisis y el onfalocele; en esta revisión se discutirá el manejo y el protocolo de seguimiento de estas dos patologías.
Standards of an Examination (cut points), Construction of Scenarios, Measurement of lmpacts in the Decision Making Process
Authors: Subhiyah Raja
Published: October 2019

The National Board of Medical Examiners (NBME®) applies rigorous examinations to determine the impact on candidates taking the United States Medical License Examination (USMLE®) for the purpose of licensing physicians in the US. UU UU These standards apply to all levels of exam development, administration and qualification. The standards apply to the following processes:• Validity of the inferences of the articles: content: the content tested must be appropriate, asking the correct questions, the format of the element, or the design of the test and the design, or the process by which the test is performed, the documentation• Accuracy of the scores: reliability, focalization, information on the cutting edge, standard errors• Determination and application of cut points: methods, modified Angoff procedure, misclassification error.The main focus is on the last standard, although the first two levels are also briefly discussed. Different methods to establish an approval standard will be discussed and the method used for USMLE will be described. Errors of misclassification are also presented and how to minimize them.
Impact Tests of UHSS Steel Welded Joints Using the Drop - Tower Impact Drop Method
Authors: Dorożyński J. , Nowacki J. , Sajek A.
Published: October 2019

The article characterizes the impact test method using Drop-Tower Impact Test with the registration of the value of force and energy of breaking. Based on sources, the possibilities and scope of the current application of this method were determined and the current state of knowledge on the results of these tests was reviewed. In order to determine the possibility of using the method in impact tests of high strength steel joints, investigations of hybrid PTA - GMA welding conditions on impact strength of joints of MART S1300QL steel were carried out. In particular, the influence of t8/5 cooling time on the impact strength of welded joints by the Drop - Tower Impact Test method was determined. It has been shown that the use of dropping machine with computer-based registration of breaking force and energy values was possible in the case of impact strength testing of UHSS welded joints and enabled precise analysis of the energy distribution dynamics absorbed by the tested.
Orbital TIG Welding of Titanium Tubes with Perforated Bottom Made of Titanium-Clad Steel
Authors: Górka J. , Przybyła M. , Szmul M. , Chudzio A. , Ładak D.
Published: October 2019

The article presents problems accompanying the industrial TIG welding (142) of a heat exchanger perforated bottom made of steel clad with titanium B265 grade 1 with tubes made of titanium B338 grade 2. Research-related tests involved the making of test plates containing simulated imperfections formed during orbital welding. The above-named imperfections resulted from insufficient gas shielding during the welding process, the improper positioning of the tungsten electrode (excessively large or overly small circumference, around which the orbital welding process was performed), an excessive electrode travel rate being the consequence of an improperly set welding programme as well as excessively high welding current. Initial tests enabled the development of the orbital TIG welding of titanium tubes with the perforated bottom made of titanium-clad steel, satisfying acceptance criteria applied during commissioning.
The Place of State Higher Vocational Schools (SHVS) from the Lublin Voivodeship in Local and Regional Development Strategies
Authors: Pyra Mariusz
Published: October 2019

Subject and purpose of work: The objective of this elaboration is to specify the place of state higher vocational schools from the Lublin voivodeship in development strategies of various territorial levels.

Materials and methods: The method of document research was applied within this elaboration, which consists of analysing the content of strategies and development plans. Within the study the induction method was also used (stage of literary studies), analysis and synthesis (stage of empirical research), deduction method (conceptual stage).

Results: References to state vocational schools occur more frequently in diagnostic segments than the programming ones of these strategic documents. Territorial self-government units (TSU) of the Chełmski subregion consider to a larger degree and thus, appreciate the functioning and public functions of vocational schools than it is the case with the Bialski or Zamojski subregions.

Conclusions: Within the regional strategic documents of the Lublin Voivodeship, statements referring to higher education refer rather to general matters. Within the local strategic documents, one may more frequently encounter more direct and literal references to the methods of possibilities of cooperation and co-action between TSU and business with state vocational schools for the benefit of local territorial arrangements.
The Eysenck Personality Profile in Selected Groups of Ophthalmological Patients
Authors: Pop-Jordanova Nada , Ristova Jovanka , Loleska Sofija
Published: October 2019

Personality correlates in patients with eye diseases have bidirectional influence. It is possible that personality variables lead to behaviours that may influence the cause of eye problems, but the opposite is also possible, that eye problems could have a direct effect on personality.

The aim of this study was to evaluate personality traits in patients with glaucoma, diabetic retinopathy and cataract using the Eysenck Personality Questionnaire.

Obtained results showed similar personality profiles in patients with glaucoma, diabetic retinopathy and cataract. However, extraversion and neuroticism were higher in patients with diabetes, while the psychopathic traits are the highest in the group of cataract.

Age and gender of patients do not influence the scores obtained on the questionnaire confirmed with one way ANOVA. Not significant difference in parameters (tested with Student t-test) was obtained between groups.

It is proposed psychological evaluation to be included in the assessment of more serious eye diseases for helping patients to adjust and adapt to the condition and treatment.
Acute Thyroiditis Associated with Brucellosis: A Case Report
Authors: Cvetkova Marija , Bitoska Iskra , Poposki Kostadin , Jakimovski Dejan , Bosilkovski Mile
Published: October 2019

Brucella thyroiditis represents an extremely rare focal form of brucellosis. In this case report we describe a 55 years old male, diagnosed with brucellosis and peripheral arthritis with subsequent development of acute thyroiditis. The symptoms duration consistent with brucellosis started two weeks before establishing the diagnosis. Only a day after diagnosis and initiation of antibrucellar treatment, acute non-suppurative thyroiditis suddenly manifested. Thyroiditis was diagnosed with clinical inspection and confirmed by ultrasound investigation. With the appropriate antibrucellar treatment, complete cure of thyroid affection was reached in ten days and the patient remained well during the follow-up period of two and a half years. In conclusion, in brucellosis endemic regions brucellosis should be included in the diagnostic consideration in patients with acute non-suppurative thyroiditis. Early recognition and adequate treatment of brucella thyroiditis results in favorable outcome.
Institutions at the Interface of Urban Planning and Real Estate
Authors: Kauko Tom
Published: October 2019

While being largely confined to different realms, planning and real estate also have a symbiotic relation. The logic of this relation varies in time and space, which makes the analysis of the balance between the two fields challenging. When we define these actions as institutions, potentially powerful analytic tools become available. This critical literature review examines some of the most relevant of them, in various specific institutional arenas, including the impact of planning on property markets and prices. The aim is to examine the role of institutions at the interface of urban planning and real estate. In other words, to see what kind of institutional effects are identifiable, whenever planning and property interests overlap. The conclusions suggest that a change in the urban fabric is likely to impact the possibility to use a site, and thereby also the value of land and built property.
Retrospective Analysis of Reoperation Rate After Standard Lumbar Discectomy and Microdiscectomy - Single Center Experience
Authors: Kovacevic Vojin , Jovanovic Nemanja
Published: October 2019

Discectomy is a surgical procedure in the treatment of lumbar disc herniation (LDH) if sciatica or neurological deficits occur and still persist after a course of conservative therapy. Standard discectomy (SD) and microdiscectomy (MD) are still equal in curent clinical practice. Many retrospective and prospective studies have shown that there is no clinically significant difference in the functional outcome after two treatment modalities.

The aim of our study was to determine whether there are differences in the incidence of reoperation after performing SD and MD.

The research included 545 patients with average period of postoperative follow-up of approximately 5.75 years. Standard discectomy was performed in 393 patients (72.11%), and micro-discectomy in 152 (27.8%) patients. The total number of reoperated patients was 37/545, or 6.78%. In the SD group, the number of reoperated patients was 33/393 (8.39%) and in the MD group 4/152 or 2.63%. Statistically significant difference (p <0.05) was recorded in favor of the MD group.

Although it has been proven that both SD and MD give good endpoints of treatment and similar functional recovery, the advantage is given to microdiscectomy due to statistically significantly lower rates of recurrent herniation. This result is attributed to better visualization of neural structures and pathological substrates, as well as their mutual relationship.
Proposal of the correlation between cartographic units on the agricultural soil map and types and subtypes of Polish Soil Classification (6th edition, 2019)
Authors: Świtoniak Marcin , Kabała Cezary , Podlasiński Marek , Smreczak Bożena
Published: October 2019

Agricultural soil maps (ASM), prepared since mid-1960s until 1980s and digitalised recently, are important source of information on the quality and spatial variability of arable soils in Poland. Basic standard information in each map contour includes the indication of a (genetic) soil type (often also the subtype or variety related to parent material or other specific properties), soil texture classes throughout the profile, and the category of soil agricultural suitability, which covers the complex information about the soil conditions, land morphology, climate and moisture regime. Unfortunately, the genetic classification on ASM is simplified compared to soil classifications in Poland and does not reflect numerous modernisations of the classification systems, including the modifications of existing units and newly introduced soil types and subtypes. Thus, the reinterpretation of ASM is necessary to simplify the further use of ASM by various users, to allow the creation of modern soil maps based on archival databases, and to correlate the soil data with other modern national and international classifications. This paper includes a proposal of equivalents for the soil units indicated in agricultural soil map (using all soil data available in a map contour), correlated with a recent, the 6th edition of Polish Soil Classification.
Pengembangan Media E-Learning Berbasis Edmodo dengan Pendekatan Saintifik pada Materi Gelombang Bunyi
Authors: Miraza Retty , Jufrida Jufrida , Pathoni Haerul
Published: October 2019

Tujuan dari penelitian ini adalah untuk menghasilkan dan mengetahui persepsi siswa terhadap pengembangan media e-learning berbasis edmodo dengan pendekatan saintifik pada materi gelombang bunyi SMA kelas XII. Jenis penelitian ini adalah penelitian dan pengembangan (research and development) dengan model 4D. Penelitian ini melalui tahap pendefenisian, perancangan, pengembangan, dan penyebaran, namun peneliti membatasi penelitian ini hanya sampai pada tahap pengembangan. Adapun yang menjadi subjek penelitian dalam pengembangan media ini yaitu siswa-siswi kelas XII MIA SMAN 2 Kota Jambi. Instrumen yang digunakan adalah angket validasi media dan materi serta angket persepsi siswa. Teknik analisis data yang berupa saran dari validator dilakukan secara deskriptif kualitatif. Sedangkan skor angket persepsi siswa dilakukan secara statistik deskriptif. Media e-learning berbasis edmodo telah selesai dikembangkan berupa media elektronik. Skor hasil persepsi siswa terhadap media e-learning berbasis edmodo yaitu 45,48 untuk aspek media termasuk kategori amat baik dan 20,02 untuk aspek materi dan termasuk kategori amat baik.Kata kunci: e-learning, gelombang bunyi The purpose of this research was producing and knowing the students perception toward the development of e-learning media based edmodo with scientific approach on  sound wave. This research was research and development with 4D model. This research  use the stage with definition, design, development, and dissemination, but the researcher limit this research only up to development stage. Subject this research were XII IPA SMAN 2 Kota Jambi. An instrument used were survey validations media, survey matter and survey students perceptions. Analysis technique of this research were deskriptive qualitative. While survey  of the students perception conducted was deskriptive statistics. E-learning media based edmodo with scientific approach has been completed as the electronic media. Scores students  perception for e-learning media was 45,58 for the aspect of media was in a very good  category and 20,02 for the material aspect was in a very good category.Keywords: e-learning, sound waves
Development of Physics Learning Tools Based on Contextual Teaching And Learning in a Remote Island Area
Authors: Ilyas Ilyas , Al Mu’min Liu An Nisaa
Published: October 2019

The research attempted to know the information on exam result Development of Physics learning tool for the students of eleven grade in Ende Island by using contextual teaching and learning in Fluid materials. It was viewed from the aspect of validity. Practicality and effectiveness. The kinds of research were development research that revers to ADDIE development model, analysis phase, design Development, implementation and evaluation. The Subject of the research was Physics learning device with approach learning model contextual teaching and learning to the students of Eleven Grade Ende Island Senior High School there are twenty nine students. Data collection was done in this research by using technique documentation. The data collection of research were done by using technique documentation, observations, survey and testing. The result showed that understanding the physics subject of fluid increased 82,7%, while students response showed positive response. This case showed that the reflection of the Egibilty of Development of Physics Learning tool based on Contextual Learning and teaching to increase the result of studying physic with Fluid materials.Keywords: Contextual Teaching and Learning, FluidaPenelitian ini bertujuan untuk mengetahui informasi hasil ujicoba perangkat pembelajaran fisika untuk peserta didik  kelas X SMAN Pulau Ende dengan menggunakan model pembelajaran Contextual Teaching and Learning pada materi fluida ditinjau dari aspek kevalidan, kepraktisan dan keefektifan. Jenis penelitian ini adalah penelitian pengembangan yang mengacu model  pengembangan ADDIE, dengan tahapan Analysis, Design, Development, Implementation, dan Evaluation. Subjek dalam penelitian ini adalah perangkat Pembelajaran fisika dengan pendekatan model pembelajran Contextual Teaching and Learning untuk peserta didik kelas XI SMAN Pulau Ende yang berjumlah 29 peserta didik.  Pengumpulan data yang dilakukan dalam penelitian ini menggunakan teknik Dokumentasi, Observasi,  dan Tes, Penyebaran Angket. Hasil penelitian menunjukkan bahwa pemahaman konsep fisika pokok bahasan fluida  mengalami peningkatan 82,7%. Sedangkan respon siswa menunjukkan respon positif. Hal ini menunjukkan kelayakan pengembangan perangkat pembelajaran fisika Berbasis Contextual Teaching and Learning untuk meningkatkan hasil belajar fisika peserta didik materi fluidaKata kunci: Contextual Teaching and Learning, Fluida