bluuebunny/crossref_metadata_2025 huggingface dataset:
High Burden of Neurodevelopmental Delay among Children Born to Women with Obstructed Labour in Eastern Uganda: A Cohort Study
Authors: Chebet Martin , Musaba Milton W. , Mukunya David , Makoko Brian , Napyo Agnes , Nantale Ritah , Auma Proscovia , Atim Ketty , Nahurira Doreck , Lee Seungwon , Okello Dedan , Ssegawa Lawrence , Bromley Kieran , Burgoine Kathy , Ndeezi Grace , Tumwine James K. , Wandabwa Julius , Kiguli Sarah
Published: February 2023

Over 250 million infants in low and middle-income countries do not fulfill their neurodevelopment potential. In this study, we assessed the incidence and risk factors for neurodevelopmental delay (NDD) among children born following obstructed labor in Eastern Uganda. Between October 2021 and April 2022, we conducted a cohort study of 155 children (aged 25 to 44 months), born at term and assessed their neurodevelopment using the Malawi Developmental Assessment Tool. We assessed the gross motor, fine motor, language and social domains of neurodevelopment. The incidence of neurodevelopmental delay by 25 to 44 months was 67.7% (105/155) (95% CI: 59.8–75.0). Children belonging to the poorest wealth quintile had 83% higher risk of NDD compared to children belonging to the richest quintile (ARR (Adjusted Risk Ratio): 1.83; 95% CI (Confidence Interval): [1.13, 2.94]). Children fed the recommended meal diversity had 25% lower risk of neurodevelopmental delay compared to children who did not (ARR: 0.75; 95% CI: [0.60, 0.94]). Children who were exclusively breastfed for the first 6 months had 27% lower risk of neurodevelopmental delay compared to children who were not (ARR: 0.73; 95% CI: [0.56, 0.96]). We recommend that infants born following obstructed labor undergo neurodevelopmental delay screening.
PCBs, PCNs, and PCDD/Fs in Soil around an Industrial Park in Northwest China: Levels, Source Apportionment, and Human Health Risk
Authors: Li Tianwei , Hu Jicheng , Xu Chenyang , Jin Jun
Published: February 2023

The concentrations of polychlorinated biphenyls (PCBs), polychlorinated naphthalenes (PCNs), and polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) were determined in soil samples collected around an industrial park in Northwest China, to investigate the potential impacts of park emissions on the surrounding environment. The total concentration ranges of PCBs, PCNs, and PCDD/Fs in the soil samples were in 13.2–1240, 141–832, and 3.60–156 pg/g, respectively. The spatial distribution and congener patterns of PCBs, PCNs, and PCCD/Fs indicated that there might be multiple contamination sources in the study area, so source apportionments of PCBs, PCNs, and PCCD/Fs were performed by a positive matrix factorization model based on the concentrations of all target congeners together. The results revealed that these highly chlorinated congeners (CB-209, CN-75, and OCDF) might be derived from phthalocyanine pigments, the legacy of Halowax 1051 and 2,4-D products, which together contributed nearly half of the total concentration of target compounds (44.5%). In addition to highly chlorinated congeners, the local industrial thermal processes were mainly responsible for the contamination of PCBs, PCNs, and PCDD/Fs in the surrounding soil. The total carcinogenic risk of PCBs, PCNs, and PCDD/Fs in a few soil samples (0.22 × 10−6, 0.32 × 10−6, and 0.40 × 10−6) approached the threshold of potential carcinogenic risk (1.0 × 10−6). Since these pollutants can continuously accumulate in the soil, the contamination of PCBs, PCNs, and PCDD/Fs in surrounding soil deserves continuous attention.
Soil Organic Matter Molecular Composition Shifts Driven by Forest Regrowth or Pasture after Slash-and-Burn of Amazon Forest
Authors: Leal Otávio dos Anjos , Jiménez-Morillo Nicasio T. , González-Pérez José A. , Knicker Heike , de Souza Costa Falberni , Jiménez-Morillo Pedro N. , de Carvalho Júnior João Andrade , dos Santos José Carlos , Pinheiro Dick Deborah
Published: February 2023

Slash-and-burn of Amazon Forest (AF) for pasture establishment has increased the occurrence of AF wildfires. Recent studies emphasize soil organic matter (SOM) molecular composition as a principal driver of post-fire forest regrowth and restoration of AF anti-wildfire ambience. Nevertheless, SOM chemical shifts caused by AF fires and post-fire vegetation are rarely investigated at a molecular level. We employed pyrolysis–gas chromatography–mass spectrometry to reveal molecular changes in SOM (0–10, 40–50 cm depth) of a slash-burn-and-20-month-regrowth AF (BAF) and a 23-year Brachiaria pasture post-AF fire (BRA) site compared to native AF (NAF). In BAF (0–10 cm), increased abundance of unspecific aromatic compounds (UACs), polycyclic aromatic hydrocarbons (PAHs) and lipids (Lip) coupled with a depletion of polysaccharides (Pol) revealed strong lingering effects of fire on SOM. This occurs despite fresh litter deposition on soil, suggesting SOM minimal recovery and toxicity to microorganisms. Accumulation of recalcitrant compounds and slow decomposition of fresh forest material may explain the higher carbon content in BAF (0–5 cm). In BRA, SOM was dominated by Brachiaria contributions. At 40–50 cm, alkyl and hydroaromatic compounds accumulated in BRA, whereas UACs accumulated in BAF. UACs and PAH compounds were abundant in NAF, possibly air-transported from BAF.
TeleRehabilitation of Social-Pragmatic Skills in Children with Autism Spectrum Disorder: A Principal Component Analysis
Authors: Marino Flavia , Failla Chiara , Bruschetta Roberta , Vetrano Noemi , Scarcella Ileana , Doria Germana , Chilà Paola , Minutoli Roberta , Vagni David , Tartarisco Gennaro , Cerasa Antonio , Pioggia Giovanni
Published: February 2023

In many therapeutic settings, remote health services are becoming increasingly a viable strategy for behavior management interventions in children with autism spectrum disorder (ASD). However, there is a paucity of tools for recovering social-pragmatic skills. In this study, we sought to demonstrate the effectiveness of a new online behavioral training, comparing the performance of an ASD group carrying out an online treatment (n°8) with respect to a control group of demographically-/clinically matched ASD children (n°8) engaged in a traditional in-presence intervention (face-to-face). After a 4-month behavioral treatment, the pragmatic skills language (APL test) abilities detected in the experimental group were almost similar to the control group. However, principal component analysis (PCA) demonstrated that the overall improvement in socio-pragmatic skills was higher for ASD children who underwent in-presence training. In fact, dimensions defined by merging APL subscale scores are clearly separated in ASD children who underwent in-presence training with respect to those performing the online approach. Our findings support the effectiveness of remote healthcare systems in managing the social skills of children with ASD, but more approaches and resources are required to enhance remote services.
Eating Competence among Brazilian College Students
Authors: Boeira Caroline Frois , Nalon de Queiroz Fabiana Lopes , Zandonadi Renata Puppin , Rower Helena Beatriz , Nakano Eduardo Yoshio , Feoli Ana Maria Pandolfo
Published: February 2023

Eating competence (EC) is a biopsychosocial concept related to a healthier eating pattern. Studies have shown that weight gain and body shape and weight dissatisfaction are typical among college students, contributing to low self-esteem, risky eating behaviors, and a propensity to develop eating disorders. As eating habits are determinant factors for food choices that can be modified by eating behavior, this study aimed to evaluate EC in college students from Brazil, using the Brazilian version of the EC Satter Inventory (ecSI2.0™BR), and EC’s association with health data. This cross-sectional study was conducted using an online survey spread using the snowball method. The self-report instrument was divided into three parts (socioeconomic and demographic data; health data; and ecSI2.0™BR). Recruitment took place through social networks, and 593 students from public and private universities from all five regions of Brazil participated in the survey. The EC average was 29.46 ± 8.67, and 46.2% of the sample were considered competent eaters. Total EC did not differ among gender and Brazilian region. Younger participants (up to 20 y/o) presented higher scores for total EC, contextual skills and food acceptance. The total EC and contextual skills of health sciences students did not differ from those of students in other areas, except for agricultural sciences in which students had lower total EC. Obese individuals and the participants who perceived themselves to be overweight had low scores for EC. This study confirmed the hypothesis that college students have low EC, causing worse health outcomes related to BMI, perceived body weight, and occurrence of hypertension and dyslipidemia.
Staying below the Radar: Unraveling a New Family of Ubiquitous “Cryptic” Non-Tailed Temperate Vibriophages and Implications for Their Bacterial Hosts
Authors: Kalatzis Panos G. , Mauritzen Jesper Juel , Winther-Have Caroline Sophie , Michniewski Slawomir , Millard Andrew , Tsertou Maria Ioanna , Katharios Pantelis , Middelboe Mathias
Published: February 2023

Bacteriophages are the most abundant biological entities in the oceans and play key roles in bacterial activity, diversity and evolution. While extensive research has been conducted on the role of tailed viruses (Class: Caudoviricetes), very little is known about the distribution and functions of the non-tailed viruses (Class: Tectiliviricetes). The recent discovery of the lytic Autolykiviridae family demonstrated the potential importance of this structural lineage, emphasizing the need for further exploration of the role of this group of marine viruses. Here, we report the novel family of temperate phages under the class of Tectiliviricetes, which we propose to name “Asemoviridae” with phage NO16 as a main representative. These phages are widely distributed across geographical regions and isolation sources and found inside the genomes of at least 30 species of Vibrio, in addition to the original V. anguillarum isolation host. Genomic analysis identified dif-like sites, suggesting that NO16 prophages recombine with the bacterial genome based on the XerCD site-specific recombination mechanism. The interactions between the NO16 phage and its V. anguillarum host were linked to cell density and phage–host ratio. High cell density and low phage predation levels were shown to favor the temperate over the lytic lifestyle for NO16 viruses, and their spontaneous induction rate was highly variable between different V. anguillarum lysogenic strains. NO16 prophages coexist with the V. anguillarum host in a mutualistic interaction by rendering fitness properties to the host, such as increased virulence and biofilm formation through lysogenic conversion, likely contributing to their global distribution.
Contribution of Glucosylsphingosine (Lyso-Gb1) to Treatment Decisions in Patients with Gaucher Disease
Authors: Dinur Tama , Bauer Peter , Beetz Christian , Cozma Claudia , Becker-Cohen Michal , Istaiti Majdolen , Rolfs Arndt , Skrahina Volha , Zimran Ari , Revel-Vilk Shoshana
Published: February 2023

Glucosylsphingosine (lyso-Gb1), the deacylated form of glucocerebroside, was shown to be the most specific and sensitive biomarker for diagnosing Gaucher disease (GD). The aim of this study is to assess the contribution of lyso-Gb1 at the time of diagnosis for treatment decisions in naïve patients with GD. Newly diagnosed patients from July 2014 to November 2022 were included in this retrospective cohort study. The diagnosis was done by sending a dry blood spot (DBS) sample for GBA1 molecular sequencing and lyso-Gb1 quantification. Treatment decisions were based on symptoms, signs, and routine laboratory tests. We diagnosed 97 patients (41 males), both type 1 (n = 87), and neuronopathic (n = 10). The median (range) age at diagnosis was 22 (1–78), with 36 children. In 65 patients, GD-specific therapy was started with a median (range) lyso-Gb1, 337 (60–1340) ng/mL, significantly higher than in patients who did not go on to treatment, 153.5 (9–442) ng/mL. Using a receiver operating characteristic (ROC) analysis, a cutoff of lyso-Gb1 > 250 ng/mL was associated with treatment with a sensitivity of 71% and specificity of 87.5%. Predictors of treatment were thrombocytopenia, anemia, and elevated lyso-Gb1 (>250 ng/mL). In conclusion, lyso-Gb1 levels contribute to the medical decision related to the initiation of treatment, mainly among mildly affected newly diagnosed patients. For patients with a severe phenotype, as for all patients, the main value of lyso-Gb1 would be to monitor response to therapy. The variable methodology and differences in the units of lyso-Gb1 measurements between laboratories prevent the adaptation of the exact cut-off we found in general practice. However, the concept is that a significant elevation, i.e., a several-fold increase from the diagnostic lyso-Gb1 cutoff, is related to a more severe phenotype and, accordingly, to the decision regarding the initiation of GD-specific therapy.
Expression of the Calcitonin Receptor-like Receptor (CALCRL) in Normal and Neoplastic Tissues
Authors: Wende Benjamin , Beyer Anna-Sophia Liselott , Ruhnke Niklas , Kaemmerer Daniel , Sänger Jörg , Schulz Stefan , Lupp Amelie
Published: February 2023

Little information is available concerning protein expression of the calcitonin receptor-like receptor (CALCRL) at the protein level. Here, we developed a rabbit monoclonal antibody, 8H9L8, which is directed against human CALCRL but cross-reacts with the rat and mouse forms of the receptor. We confirmed antibody specificity via Western blot analyses and immunocytochemistry using the CALCRL-expressing neuroendocrine tumour cell line BON-1 and a CALCRL-specific small interfering RNA (siRNA). We then used the antibody for immunohistochemical analyses of various formalin-fixed, paraffin-embedded specimens of normal and neoplastic tissues. In nearly all tissue specimens examined, CALCRL expression was detected in the capillary endothelium, smooth muscles of the arterioles and arteries, and immune cells. Analyses of normal human, rat, and mouse tissues revealed that CALCRL was primarily present in distinct cell populations in the cerebral cortex; pituitary; dorsal root ganglia; epithelia, muscles, and glands of the larger bronchi; intestinal mucosa (particularly in enteroendocrine cells); intestinal ganglia; exocrine and endocrine pancreas; arteries, capillaries, and glomerular capillary loops in the kidneys; the adrenals; Leydig cells in the testicles; and syncytiotrophoblasts in the placenta. In the neoplastic tissues, CALCRL was predominantly expressed in thyroid carcinomas, parathyroid adenomas, small-cell lung cancers, large-cell neuroendocrine carcinomas of the lung, pancreatic neuroendocrine neoplasms, renal clear-cell carcinomas, pheochromocytomas, lymphomas, and melanomas. In these tumours with strong expression of CALCRL, the receptor may represent a useful target structure for future therapies.
Cross-Reactivity of Intraoral Allergic Contact Mucositis in the Nickel-Sensitized Ear Model of Metal Allergy
Authors: Matsubara Ryota , Kumagai Kenichi , Nasu Keisuke , Yoshizawa Takamasa , Kitaura Kazutaka , Suzuki Motoaki , Hamada Yoshiki , Suzuki Ryuji
Published: February 2023

Cross-reactivity of metal allergies can make metal allergy treatment complicated because the background of immune response in cross-reactions remains unknown. In clinical settings, cross-reactivity among several metals has been suspected. However, the precise mechanism of immune response in cross-reactivity is unclear. Two sensitizations with nickel, palladium, and chromium plus lipopolysaccharide solution into the postauricular skin were followed by a single nickel, palladium, and chromium challenge of the oral mucosa to generate the intraoral metal contact allergy mouse model. Results showed that the infiltrating T cells in nickel-sensitized, palladium- or chromium-challenged mice expressed CD8+ cells, cytotoxic granules, and inflammation-related cytokines. Thus, nickel ear sensitization can cause cross-reactive intraoral metal allergy.
Multichromosomal Mitochondrial Genome of Paphiopedilum micranthum: Compact and Fragmented Genome, and Rampant Intracellular Gene Transfer
Authors: Yang Jia-Xing , Dierckxsens Nicolas , Bai Ming-Zhu , Guo Yan-Yan
Published: February 2023

Orchidaceae is one of the largest families of angiosperms. Considering the large number of species in this family and its symbiotic relationship with fungi, Orchidaceae provide an ideal model to study the evolution of plant mitogenomes. However, to date, there is only one draft mitochondrial genome of this family available. Here, we present a fully assembled and annotated sequence of the mitochondrial genome (mitogenome) of Paphiopedilum micranthum, a species with high economic and ornamental value. The mitogenome of P. micranthum was 447,368 bp in length and comprised 26 circular subgenomes ranging in size from 5973 bp to 32,281 bp. The genome encoded for 39 mitochondrial-origin, protein-coding genes; 16 tRNAs (three of plastome origin); three rRNAs; and 16 ORFs, while rpl10 and sdh3 were lost from the mitogenome. Moreover, interorganellar DNA transfer was identified in 14 of the 26 chromosomes. These plastid-derived DNA fragments represented 28.32% (46,273 bp) of the P. micranthum plastome, including 12 intact plastome origin genes. Remarkably, the mitogenome of P. micranthum and Gastrodia elata shared 18% (about 81 kb) of their mitochondrial DNA sequences. Additionally, we found a positive correlation between repeat length and recombination frequency. The mitogenome of P. micranthum had more compact and fragmented chromosomes compared to other species with multichromosomal structures. We suggest that repeat-mediated homologous recombination enables the dynamic structure of mitochondrial genomes in Orchidaceae.
The Pre-Stroke Induction and Normalization of Insulin Resistance Respectively Worsens and Improves Functional Recovery
Authors: Vercalsteren Ellen , Karampatsi Dimitra , Dekens Doortje , Letsiou Aikaterini , Zabala Alexander , Romanitan Mihaela , Klein Thomas , Nyström Thomas , Darsalia Vladimer , Patrone Cesare
Published: February 2023

Type 2 diabetes (T2D) impairs post-stroke recovery, and the underlying mechanisms are unknown. Insulin resistance (IR), a T2D hallmark that is also closely linked to aging, has been associated with impaired post-stroke recovery. However, whether IR worsens stroke recovery is unknown. We addressed this question in mouse models where early IR, with or without hyperglycemia, was induced by chronic high-fat diet feeding or sucrose supplementation in the drinking water, respectively. Furthermore, we used 10-month-old mice, spontaneously developing IR but not hyperglycemia, where IR was normalized pharmacologically pre-stroke with Rosiglitazone. Stroke was induced by transient middle cerebral artery occlusion and recovery was assessed by sensorimotor tests. Neuronal survival, neuroinflammation and the density of striatal cholinergic interneurons were also assessed by immunohistochemistry/quantitative microscopy. Pre-stroke induction and normalization of IR, respectively, worsened and improved post-stroke neurological recovery. Moreover, our data indicate a potential association of this impaired recovery with exacerbated neuroinflammation and a decreased density of striatal cholinergic interneurons. The global diabetes epidemic and population aging are dramatically increasing the percentage of people in need of post-stroke treatment/care. Our results suggest that future clinical studies should target pre-stroke IR to reduce stroke sequelae in both diabetics and elderly people with prediabetes.
Understanding the Exchange Interaction between Paramagnetic Metal Ions and Radical Ligands: DFT and Ab Initio Study on Semiquinonato Cu(II) Complexes
Authors: Ziółkowska Aleksandra , Witwicki Maciej
Published: February 2023

The exchange coupling, represented by the J parameter, is of tremendous importance in understanding the reactivity and magnetic behavior of open-shell molecular systems. In the past, it was the subject of theoretical investigations, but these studies are mostly limited to the interaction between metallic centers. The exchange coupling between paramagnetic metal ions and radical ligands has hitherto received scant attention in theoretical studies, and thus the understanding of the factors governing this interaction is lacking. In this paper, we use DFT, CASSCF, CASSCF/NEVPT2, and DDCI3 methods to provide insight into exchange interaction in semiquinonato copper(II) complexes. Our primary objective is to identify structural features that affect this magnetic interaction. We demonstrate that the magnetic character of Cu(II)-semiquinone complexes are mainly determined by the relative position of the semiquinone ligand to the Cu(II) ion. The results can support the experimental interpretation of magnetic data for similar systems and can be used for the in-silico design of magnetic complexes with radical ligands.
Subtask Segmentation Methods of the Timed Up and Go Test and L Test Using Inertial Measurement Units—A Scoping Review
Authors: McCreath Frangakis Alexis L. , Lemaire Edward D. , Baddour Natalie
Published: February 2023

The Timed Up and Go test (TUG) and L Test are functional mobility tests that allow healthcare providers to assess a person’s balance and fall risk. Segmenting these mobility tests into their respective subtasks, using sensors, can provide further and more precise information on mobility status. To identify and compare current methods for subtask segmentation using inertial sensor data, a scoping review of the literature was conducted using PubMed, Scopus, and Google Scholar. Articles were identified that described subtask segmentation methods for the TUG and L Test using only inertial sensor data. The filtering method, ground truth estimation device, demographic, and algorithm type were compared. One article segmenting the L Test and 24 articles segmenting the TUG met the criteria. The articles were published between 2008 and 2022. Five studies used a mobile smart device’s inertial measurement system, while 20 studies used a varying number of external inertial measurement units. Healthy adults, people with Parkinson’s Disease, and the elderly were the most common demographics. A universally accepted method for segmenting the TUG test and the L Test has yet to be published. Angular velocity in the vertical and mediolateral directions were common signals for subtask differentiation. Increasing sample sizes and furthering the comparison of segmentation methods with the same test sets will allow us to expand the knowledge generated from these clinically accessible tests.
Adaptive Savitzky–Golay Filters for Analysis of Copy Number Variation Peaks from Whole-Exome Sequencing Data
Authors: Ochieng Peter Juma , Maróti Zoltán , Dombi József , Krész Miklós , Békési József , Kalmár Tibor
Published: February 2023

Copy number variation (CNV) is a form of structural variation in the human genome that provides medical insight into complex human diseases; while whole-genome sequencing is becoming more affordable, whole-exome sequencing (WES) remains an important tool in clinical diagnostics. Because of its discontinuous nature and unique characteristics of sparse target-enrichment-based WES data, the analysis and detection of CNV peaks remain difficult tasks. The Savitzky–Golay (SG) smoothing is well known as a fast and efficient smoothing method. However, no study has documented the use of this technique for CNV peak detection. It is well known that the effectiveness of the classical SG filter depends on the proper selection of the window length and polynomial degree, which should correspond with the scale of the peak because, in the case of peaks with a high rate of change, the effectiveness of the filter could be restricted. Based on the Savitzky–Golay algorithm, this paper introduces a novel adaptive method to smooth irregular peak distributions. The proposed method ensures high-precision noise reduction by dynamically modifying the results of the prior smoothing to automatically adjust parameters. Our method offers an additional feature extraction technique based on density and Euclidean distance. In comparison to classical Savitzky–Golay filtering and other peer filtering methods, the performance evaluation demonstrates that adaptive Savitzky–Golay filtering performs better. According to experimental results, our method effectively detects CNV peaks across all genomic segments for both short and long tags, with minimal peak height fidelity values (i.e., low estimation bias). As a result, we clearly demonstrate how well the adaptive Savitzky–Golay filtering method works and how its use in the detection of CNV peaks can complement the existing techniques used in CNV peak analysis.
Stochastic Multiphasic Multivariate State-Based Degradation and Maintenance Meta-Models for RC Structures Subject to Chloride Ingress
Authors: El Hajj Boutros , Castanier Bruno , Schoefs Franck , Bastidas-Arteaga Emilio
Published: February 2023

The objective of this paper is to propose tools for the lifecycle management of infrastructure by showing the slow degradation processes for which inspection data are accessible, especially the data obtained from non-destructive testing (NDT) and structural health monitoring (SHM). One major characteristic of these degradation processes is their multiphasic nature; consequently, they can be discretised into different phases with specific physical kinematics where specific maintenance actions and measurement techniques can be performed. Within this framework, we propose implementing a degradation meta-modelling approach fed with measurements (NDT, SHM). This approach is based on state-dependent stochastic processes for modelling the degradation and maintenance of reinforced concrete structures that are subjected to chloride-induced deterioration. The benefit of using multiphasic degradation meta-models in the lifecycle management of infrastructure is illustrated through numerical examples that include single and multi-action management policies.
Parthenogenetic Reproduction in Strumigenys Ants: An Update
Authors: Wang Chu , Sung Ping-Jui , Lin Chung-Chi , Ito Fuminori , Billen Johan
Published: February 2023

Parthenogenetic reproduction is a common feature for social Hymenoptera, as males typically develop from unfertilized eggs (arrhenotoky). Production of female offspring without the involvement of sperm (thelytoky) also exists but is rather exceptional as it has been reported for only 16 ant species so far. Three of these belong to the genus Strumigenys: S. hexamera, S. membranifera and S. rogeri. Our observations on the reproductive biology in various Oriental Strumigenys species extends this list of thelytokous ants with three more species: S. emmae, S. liukueiensis and S. solifontis. Of these six thelotykous species, S. emmae, S. membranifera and S. rogeri are known as tramp species. Reproduction without the need to fertilize eggs no doubt offers these species a considerable advantage when establishing colonies in new environments. Published histological data on S. hexamera and S. membranifera already showed that the queens possess a functional spermatheca. We now provide evidence that this is also the case for the four other thelytokous Strumigenys species. Retaining a functional spermatheca and reproductive system may keep the queens ready for the exceptional event of mating and hence increase genetic variability, as males do occur very rarely.
Biology, Ecology and Management of Tephritid Fruit Flies in China: A Review
Authors: He Yuxin , Xu Yijuan , Chen Xiao
Published: February 2023

Tephritid fruit flies are notoriously known for causing immense economic losses due to their infestation of many types of commercial fruits and vegetables in China. These flies are expanding, causing serious damage, and we summarized references from the last three decades regarding biological parameters, ecological performance and integrated pest management. There are 10 species of tephritid fruit flies mentioned at a relatively high frequency in China, and a detailed description and discussion in this comprehensive review were provided through contrast and condensation, including economics, distribution, identification, hosts, damage, life history, oviposition preference, interspecific competition and integrated management, in anticipation of providing effective strategies or bases for the subsequent development of new research areas and improvement of integrated management systems.
Influence of Soldiers on Exploratory Foraging Behavior in the Formosan Subterranean Termite, Coptotermes formosanus (Blattodea: Rhinotermitidae)
Authors: McCarthy Joseph , Khadka Arjun , Hakanoglu Hasim , Sun Qian
Published: February 2023

Termites are eusocial insects that live in organized colonies consisting of reproductives, workers, and soldiers. Soldiers are specialized for defense but are expensive to maintain, as they are incapable of husbandry and must be fed and groomed by workers. The soldiers of several species influence foraging behavior by acting as scouts that initiate foraging or by mediating worker behavioral plasticity during food exploration. These behaviors imply that soldiers may play a keystone role in termite colony function, apart from defense. Subterranean termite workers tunnel through soil in search of food while accompanied by varying proportions of soldiers, depending on the species and colony conditions. Previous studies have shown that soldiers accelerate worker exploratory tunneling behavior in two Reticulitermes species, the colonies of which contain fewer than 2% soldiers. This effect, however, is unknown in other subterranean species with different soldier proportions. In this study, we examined the influence of soldiers on exploratory foraging behavior in the Formosan subterranean termite, Coptotermes formosanus Shiraki, which is an economically devastating invasive species that maintains a relatively high soldier proportion (about 10%). When 100 foraging workers were grouped with 0, 2, 10, or 30 soldiers in two-dimensional foraging arenas, we found no significant effect of soldiers on the tunnel length, branch pattern, food source interception, or food collected within 96 h. These results suggest that C. formosanus colonies maintain food exploration efficiency regardless of soldier proportion variation.
Pregnancy after Kidney Transplantation—Impact of Functional Renal Reserve, Slope of eGFR before Pregnancy, and Intensity of Immunosuppression on Kidney Function and Maternal Health
Authors: Kaatz Rebecca , Latartara Elisabetta , Bachmann Friederike , Lachmann Nils , Koch Nadine , Zukunft Bianca , Wu Kaiyin , Schmidt Danilo , Halleck Fabian , Nickel Peter , Eckardt Kai-Uwe , Budde Klemens , Verlohren Stefan , Choi Mira
Published: February 2023

Women of childbearing age show increased fertility after kidney transplantation. Of concern, preeclampsia, preterm delivery, and allograft dysfunction contribute to maternal and perinatal morbidity and mortality. We performed a retrospective single-center study, including 40 women with post-transplant pregnancies after single or combined pancreas–kidney transplantation between 2003 and 2019. Outcomes of kidney function up to 24 months after the end of pregnancy were compared with a matched-pair cohort of 40 transplanted patients without pregnancies. With a maternal survival rate of 100%, 39 out of 46 pregnancies ended up with a live-born baby. The eGFR slopes to the end of 24 months follow-up showed mean eGFR declines in both groups (−5.4 ± 14.3 mL/min in pregnant versus −7.6 ± 14.1 mL/min in controls). We identified 18 women with adverse pregnancy events, defined as preeclampsia with severe end-organ dysfunction. An impaired hyperfiltration during pregnancy was a significant risk contributor for both adverse pregnancy events (p < 0.05) and deterioration of kidney function (p < 0.01). In addition, a declining renal allograft function in the year before pregnancy was a negative predictor of worsening allograft function after 24 months of follow-up. No increased frequency of de novo donor-specific antibodies after delivery could be detected. Overall, pregnancies in women after kidney transplantation showed good allograft and maternal outcomes.
Morphological Medial Gastrocnemius Muscle Growth in Ambulant Children with Spastic Cerebral Palsy: A Prospective Longitudinal Study
Authors: De Beukelaer Nathalie , Vandekerckhove Ines , Huyghe Ester , Molenberghs Geert , Peeters Nicky , Hanssen Britta , Ortibus Els , Van Campenhout Anja , Desloovere Kaat
Published: February 2023

Only cross-sectional studies have demonstrated muscle deficits in children with spastic cerebral palsy (SCP). The impact of gross motor functional limitations on altered muscle growth remains unclear. This prospective longitudinal study modelled morphological muscle growth in 87 children with SCP (age range 6 months to 11 years, Gross Motor Function Classification System [GMFCS] level I/II/III = 47/22/18). Ultrasound assessments were performed during 2-year follow-up and repeated for a minimal interval of 6 months. Three-dimensional freehand ultrasound was applied to assess medial gastrocnemius muscle volume (MV), mid-belly cross-sectional area (CSA) and muscle belly length (ML). Non-linear mixed models compared trajectories of (normalized) muscle growth between GMFCS-I and GMFCS-II&III. MV and CSA growth trajectories showed a piecewise model with two breakpoints, with the highest growth before 2 years and negative growth rates after 6–9 years. Before 2 years, children with GMFCS-II&III already showed lower growth rates compared to GMFCS-I. From 2 to 9 years, the growth rates did not differ between GMFCS levels. After 9 years, a more pronounced reduction in normalized CSA was observed in GMFCS-II&III. Different trajectories in ML growth were shown between the GMFCS level subgroups. These longitudinal trajectories highlight monitoring of SCP muscle pathology from early ages and related to motor mobility. Treatment planning and goals should stimulate muscle growth.
The Increase of Theta Power and Decrease of Alpha/Theta Ratio as a Manifestation of Cognitive Impairment in Parkinson’s Disease
Authors: Zawiślak-Fornagiel Katarzyna , Ledwoń Daniel , Bugdol Monika , Romaniszyn-Kania Patrycja , Małecki Andrzej , Gorzkowska Agnieszka , Mitas Andrzej W.
Published: February 2023

In this study, we aim to assess and examine cognitive functions in Parkinson’s Disease patients using EEG recordings, with a central focus on characteristics associated with a cognitive decline. Based on neuropsychological evaluation using Mini-Mental State Examination, Montreal Cognitive Assessment, and Addenbrooke’s Cognitive Examination-III, 98 participants were divided into three cognitive groups. All the particpants of the study underwent EEG recordings with spectral analysis. The results revealed an increase in the absolute theta power in patients with Parkinson’s disease dementia (PD-D) compared to cognitively normal status (PD-CogN, p=0.00997) and a decrease in global relative beta power in PD-D compared to PD-CogN (p=0.0413). An increase in theta relative power in the left temporal region (p=0.0262), left occipital region (p=0.0109), and right occipital region (p=0.0221) were observed in PD-D compared to PD-N. The global alpha/theta ratio and global power spectral ratio significantly decreased in PD-D compared to PD-N (p = 0.001). In conclusion, the increase in relative theta power and the decrease in relative beta power are characteristic changes in EEG recordings in PD patients with cognitive impairment. Identifying these changes can be a useful biomarker and a complementary tool in the neuropsychological diagnosis of cognitive impairment in Parkinson’s Disease.
A New Perspective on the Diagnosis of Septic Arthritis: High-Resolution Thermal Imaging
Authors: Gunay Huseyin , Bakan Ozgur Mert , Mirzazade Javad , Sozbilen Murat Celal
Published: February 2023

Aims: An increase in temperature in an area suspected of septic arthritis is a clinically important finding. The aim of this study is to evaluate temperature changes in septic arthritis with a high-resolution thermal camera. Methods: A total of 49 patients, who were evaluated with a prediagnosis of arthritis (septic or non-septic), were included in this study. A temperature increase in the knee with suspected septic arthritis was evaluated by using thermal imaging and compared with the opposite-side joint. Then, in order to confirm the diagnosis, a culture was taken using routine intra-articular aspiration. Results: The thermal measurements were compared in 15 patients with septic arthritis and 34 patients with non-septic arthritis. The mean temperature was 37.93 °C in the septic group, while it was 36.79 °C in the non-septic group (p < 0.000 *). The mean temperature difference in both joints was 3.40 °C in the septic group, while it was 0.94 °C in the non-septic group (p < 0.000 *). While the mean temperature was 37.10 °C in the group with septic arthritis, it was measured to be 35.89 °C in the group non-septic arthritis (p < 0.020). A very strong positive correlation was found between the difference in the mean temperatures of both groups and the values of the hottest and coldest points (r = 0.960, r = 0.902). Conclusions: In the diagnosis of septic arthritis, thermal imagers can be used as a non-invasive diagnostic tool. A quantitative value can be obtained to indicate to a local temperature increase. In future studies, specially designed thermal devices can be developed for septic arthritis.
Cardiovascular Magnetic Resonance Demonstrates Myocardial Inflammation of Differing Etiologies and Acuities in Patients with Genetic and Inflammatory Myopathies
Authors: Markousis-Mavrogenis George , Belegrinos Antonios , Giannakopoulou Aikaterini , Papavasiliou Antigoni , Koulouri Vasiliki , Marketos Nikolaos , Patsilinakou Eleftheria , Lazarioti Fotini , Bacopoulou Flora , Mavragani Clio P. , Chrousos George P. , Mavrogeni Sophie I.
Published: February 2023

Introduction. Myopathies are heterogeneous neuromuscular diseases of genetic and/or inflammatory etiology that affect both cardiac and skeletal muscle. We investigated the prevalence of cardiac inflammation in patients with myopathies, cardiovascular symptoms, and normal echocardiography using cardiovascular magnetic resonance (CMR). Methods. We prospectively evaluated 51 patients with various genetic (n = 23) and inflammatory (n = 28) myopathies (median age, IQR: 12 (11–15) years, 22% girls; 61 (55–65) years, 46% women, respectively) and compared their CMR findings to corresponding age- and sex-matched controls (n = 21 and 20, respectively) and to each other. Results. Patients with genetic myopathy had similar biventricular morphology and function to healthy controls but showed higher late gadolinium enhancement (LGE), native T1 mapping, extracellular volume fraction (ECV), and T2 mapping values. Collectively, 22 (95.7%) patients with genetic myopathy had a positive T1-criterion and 3 (13.0%) had a positive T2-criterion according to the updated Lake Louise criteria. Compared with healthy controls, patients with inflammatory myopathy showed preserved left ventricular (LV) function and reduced LV mass, while all CMR-derived tissue characterization indices were significantly higher (p < 0.001 for all). All patients had a positive T1-criterion, and 27 (96.4%) had a positive T2-criterion. A positive T2-criterion or T2-mapping > 50 ms could discriminate between patients with genetic and inflammatory myopathies with a sensitivity of 96.4% and a specificity of 91.3% (AUC = 0.9557). Conclusions. The vast majority of symptomatic patients with inflammatory myopathies and normal echocardiography show evidence of acute myocardial inflammation. In contrast, acute inflammation is rare in patients with genetic myopathies, who show evidence of chronic low-grade inflammation.
The Cybersecurity Awareness INventory (CAIN): Early Phases of Development of a Tool for Assessing Cybersecurity Knowledge Based on the ISO/IEC 27032
Authors: Tempestini Giorgia , Rovira Ericka , Pyke Aryn , Di Nocera Francesco
Published: February 2023

Knowledge of possible cyber threats as well as awareness of appropriate security measures plays a crucial role in the ability of individuals to not only discriminate between an innocuous versus a dangerous cyber event, but more importantly to initiate appropriate cybersecurity behaviors. The purpose of this study was to construct a Cybersecurity Awareness INventory (CAIN) to be used as an instrument to assess users’ cybersecurity knowledge by providing a proficiency score that could be correlated with cyber security behaviors. A scale consisting of 46 items was derived from ISO/IEC 27032. The questionnaire was administered to a sample of college students (N = 277). Based on cybersecurity behaviors reported to the research team by the college’s IT department, each participant was divided into three groups according to the risk reports they received in the past nine months (no risk, low risk, and medium risk). The ANOVA results showed a statistically significant difference in CAIN scores between those in the no risk and medium-risk groups; as expected, CAIN scores were lower in the medium-risk group. The CAIN has the potential to be a useful assessment tool for cyber training programs as well as future studies investigating individuals’ vulnerability to cyberthreats.
Whole CNS 3D Cryo-Fluorescence Tomography Shows CSF Clearance along Nasal Lymphatics, Spinal Nerves, and Lumbar/Sacral Lymph Nodes
Authors: Stokes Christian , White Eli F , Toddes Steve , Bens Nicole , Kulkarni Praveen , Ferris Craig F
Published: February 2023

Unwanted proteins and metabolic waste in cerebral spinal fluid are cleared from the brain by meningeal and nasal lymphatics and the perineural sheath of cranial nerves; however, the distribution and clearance of cerebral spinal fluid (CSF) along the subarachnoid space of the entire spinal cord is not fully understood. Cryo-fluorescence tomography (CFT) was used to follow the movement of tracers from the ventricular system of the brain down through the meningeal lining of the spinal cord and out to the spinal lymphatic nodes. Isoflurane-anesthetized mice were infused into the lateral cerebroventricle with 5.0 µL of quantum dots [QdotR 605 ITKTM amino (PEG)] over two mins. Mice were allowed to recover (ca 2–3 min) and remained awake and ambulatory for 5, 15, 30, 60, and 120 min after which they were euthanized, and the entire intact body was frozen at −80°. The entire mouse was sectioned, and white light and fluorescent images were captured after each slice to produce high resolution three-dimensional volumes. Tracer appeared throughout the ventricular system and central canal of the spinal cord and the entire subarachnoid space of the CNS. A signal could be visualized in the nasal cavity, deep cervical lymph nodes, thoracic lymph nodes, and more superficial submandibular lymph nodes as early as 15 min post infusion. A fluorescent signal could be visualized along the dorsal root ganglia and down the proximal extension of the spinal nerves of the thoracic and lumbar segments at 30 min. There was a significant accumulation of tracer in the lumbar and sacral lymph nodes between 15–60 min. The dense fluorescent signal in the thoracic vertebrae noted at 5- and 15-min post infusion was significantly reduced by 30 min. Indeed, all signals in the spinal cord were ostensibly absent by 120 min, except for trace amounts in the coccyx. The brain still had some residual signal at 120 min. These data show that Qdots with a hydrodynamic diameter of 16–20 nm rapidly clear from the brain of awake mice. These data also clearly demonstrate the rapid distribution and efflux of traces along a major length of the vertebral column and the potential contribution of the spinal cord in the clearance of brain waste.
A Log File Analysis on the Validity of Partial Solutions in Figural Matrices Tests
Authors: Weber Dominik , Krieger Florian , Spinath Frank M. , Greiff Samuel , Hissbach Johanna , Becker Nicolas
Published: February 2023

As a component of many intelligence test batteries, figural matrices tests are an effective way to assess reasoning, which is considered a core ability of intelligence. Traditionally, the sum of correct items is used as a performance indicator (total solution procedure). However, recent advances in the development of computer-based figural matrices tests allow additional indicators to be considered for scoring. In two studies, we focused on the added value of a partial solution procedure employing log file analyses from a computer-based figural matrices test. In the first study (n = 198), we explored the internal validity of this procedure by applying both an exploratory bottom-up approach (using sequence analyses) and a complementary top-down approach (using rule jumps, an indicator taken from relevant studies). Both approaches confirmed that higher scores in the partial solution procedure were associated with higher structuredness in participants’ response behavior. In the second study (n = 169), we examined the external validity by correlating the partial solution procedure in addition to the total solution procedure with a Grade Point Average (GPA) criterion. The partial solution procedure showed an advantage over the total solution procedure in predicting GPA, especially at lower ability levels. The implications of the results and their applicability to other tests are discussed.
Life Cycle Cost Analysis for Scotland Short-Sea Ferries
Authors: Hwang Insik , Park Chybyung , Jeong Byongug
Published: February 2023

The pathway to zero carbon emissions passing through carbon emissions reduction is mandatory in the shipping industry. Regarding the various methodologies and technologies reviewed for this purpose, Life Cycle Cost Analysis (LCCA) has been used as an excellent tool to determine economic feasibility and sustainability and to present directions. However, insufficient commercial applications cause a conflict of opinion on which fuel is the key to decarbonisation. Many LCCA comparison studies about eco-friendly ship propulsion claim different results. In order to overcome this and discover the key factors that affect the overall comparative analysis and results in the maritime field, it is necessary to conduct the comparative analysis considering more diverse case ships, case routes, and various types that combine each system. This study aims to analyse which greener fuels are most economically beneficial for the shipping sector and prove the factors influencing different results in LCCA. This study was conducted on hydrogen, ammonia, and electric energy, which are carbon-free fuels among various alternative fuels that are currently in the limelight. As the power source, a PEMFC and battery were used as the main power source, and a solar PV system was installed as an auxiliary power source to compare economic feasibility. Several cost data for LCCA were selected from various feasible case studies. As the difficulty caused by the storage and transportation of hydrogen and ammonia should not be underestimated, in this study, the LCCA considers not only the CapEx and OpEx but also fuel transport costs. As a result, fuel cell propulsion systems with hydrogen as fuel proved financial effectiveness for short-distance ferries as they are more inexpensive than ammonia-fuelled PEMFCs and batteries. The fuel cost takes around half of the total life-cycle cost during the life span.