bluuebunny/crossref_metadata_2025 huggingface dataset:
The Fitter the Better? Cardiopulmonary Exercise Testing Can Predict Pulmonary Exacerbations in Cystic Fibrosis
Authors: Kampouras Asterios , Hatziagorou Elpis , Kalantzis Thomas , Avramidou Vasiliki , Kontouli Kalliopi , Kirvassilis Fotios , Tsanakas John
Published: June 2021

Background: The role of cardiopulmonary exercise testing (CPET) in the assessment of prognosis in CF (cystic fibrosis) is crucial. However, as the overall survival of the disease becomes better, the need for examinations that can predict pulmonary exacerbations (PEx) and subsequent deterioration becomes evident. Methods: Data from a 10-year follow up with CPET and spirometry of CF patients were used to evaluate whether CPET-derived parameters can be used as prognostic indexes for pulmonary exacerbations in patients with CF. Pulmonary exacerbations were recorded. We used a survival analysis through Cox Regression to assess the prognostic role of CPET parameters for PeX. CPET parameters and other variables such as sputum culture, age, and spirometry measurements were tested via multivariate cox models. Results: During a 10-year period (2009–2019), 78 CF patients underwent CPET. Cox regression analysis revealed that VO2peak% (peak Oxygen Uptake predicted %) predicted (hazard ratio (HR), 0.988 (0.975, 1.000) p = 0.042) and PetCO2 (end-tidal CO2 at peak exercise) (HR 0.948 (0.913, 0.984) p = 0.005), while VE/VO2 and (respiratory equivalent for oxygen at peak exercise) (HR 1.032 (1.003, 1.062) p = 0.033) were significant predictors of pulmonary exacerbations in the short term after the CPET. Additionally, patients with VO2peak% predicted <60% had 4.5-times higher relative risk of having a PEx than those with higher exercise capacity. Conclusions: CPET can provide valuable information regarding upcoming pulmonary exacerbation in CF. Patients with VO2peak <60% are at great risk of subsequent deterioration. Regular follow up of CF patients with exercise testing can highlight their clinical image and direct therapeutic interventions.
Global Sensitivity Analysis Based on Entropy: From Differential Entropy to Alternative Measures
Authors: Kala Zdeněk
Published: June 2021

Differential entropy can be negative, while discrete entropy is always non-negative. This article shows that negative entropy is a significant flaw when entropy is used as a sensitivity measure in global sensitivity analysis. Global sensitivity analysis based on differential entropy cannot have negative entropy, just as Sobol sensitivity analysis does not have negative variance. Entropy is similar to variance but does not have the same properties. An alternative sensitivity measure based on the approximation of the differential entropy using dome-shaped functionals with non-negative values is proposed in the article. Case studies have shown that new sensitivity measures lead to a rational structure of sensitivity indices with a significantly lower proportion of higher-order sensitivity indices compared to other types of distributional sensitivity analysis. In terms of the concept of sensitivity analysis, a decrease in variance to zero means a transition from the differential to discrete entropy. The form of this transition is an open question, which can be studied using other scientific disciplines. The search for new functionals for distributional sensitivity analysis is not closed, and other suitable sensitivity measures may be found.
GIS in Architectural Teaching and Research: Planning and Heritage
Authors: Santos Bertha , Gonçalves Jorge , Martins Ana M. , Pérez-Cano Maria T. , Mosquera-Adell Eduardo , Dimelli Despina , Lagarias Apostolos , Almeida Pedro G.
Published: June 2021

Geographical Information Systems (GISs) in architecture were initially limited to regional and urban development applications. Over recent years its potential has been recognized and its use has evolved to address urban planning and architectural heritage management subjects. Nevertheless, evidence shows that its use in architecture teaching is scarce and uneven. Directive 2007/2/EC, establishing the infrastructure for spatial information in the European Community (EU), is, in this way, an opportunity to develop a greater knowledge and application of GIS in the framework of higher education. In architecture, this can be achieved by problem solving based on real case scenarios, which can benefit from GIS-based techniques and analysis capabilities. In this paper, the authors aim to present a review of the use of GIS in teaching and research in architecture to assess its level in different European programmes. Experiences from three European universities (University of Seville, Spain, University of Beira Interior, Portugal, and Technical University of Crete, Greece), which are among the few in their respective countries that promote the inclusion of GIS in architectural education, particularly in the fields of urban and regional planning and architectural heritage, are compared and framed within the European scenario. The paper concludes with a reflection on the three universities’ practice compared to the leading European architecture programmes listed in the main international university rankings. Main trends of future evolution on the use of GIS in architecture teaching are also presented.
On Approaching Relevant Cost-Effective Sustainable Maintenance of Mineral Oil-Filled Electrical Transformers
Authors: Jadim Ramsey , Kans Mirka , Schulte Jesko , Alhattab Mohammed , Alhendi May , Bushehry Ali
Published: June 2021

Fire and explosion accidents of oil-filled electrical transformers are leading to negative impacts, not only on the delivery of energy, but also on workplace health and safety as well as the surrounding environment. Such accidents are still being reported, regardless of applying the regular maintenance strategy in the power plants. The purpose of this paper is to integrate a sustainability perspective into the maintenance strategy. The problem addressed is: how can we approach the relevant cost-effective sustainable maintenance for oil-filled electrical transformers? For this purpose, an empirical study in a power plant in Kuwait was introduced. The first stage was to carry out a sustainability assessment using the ABCD procedure. In this procedure, gaps to approach sustainability were identified and actions prioritized to close these gaps were demonstrated. Applying this procedure yielded an early fault diagnosis (EFD) model for achieving cost-effective sustainable maintenance using a fault trend chart based on a novel numerical method. Implementing this model resulted in an extension of the lifetime of transformers with suspected failure propagation, leading to a deferral of the replacement investment costs. The principal conclusion of this paper is the importance of viewing the maintenance strategy of transformers from a strategic sustainability perspective, in order to approach relevant cost-effective sustainable maintenance.
Chemical Degradation of the La0.6Sr0.4Co0.2Fe0.8O3−δ/Ce0.8Sm0.2O2−δ Interface during Sintering and Cell Operation
Authors: François Mélanie , Carpanese Maria Paola , Heintz Olivier , Lescure Victoire , Clematis Davide , Combemale Lionel , Demoisson Frédéric , Caboche Gilles
Published: June 2021

A complete cell consisting of NiO-Ce0.8Sm0.2O3−δ//Ce0.8Sm0.2O3−δ//(La0.6Sr0.4)0.95Co0.2Fe0.8O3−δ elaborated by a co-tape casting and co-sintering process and tested in operating fuel cell conditions exhibited a strong degradation in performance over time. Study of the cathode–electrolyte interface after cell testing showed, on one hand, the diffusion of lanthanum from (La0.6Sr0.4)0.95Co0.2Fe0.8O3−δ into Sm-doped ceria leading to a La- and Sm-doped ceria phase. On the other hand, Ce and Sm diffused into the perovskite phase of the cathode. The grain boundaries appear to be the preferred pathways of the cation diffusion. Furthermore, a strontium enrichment was clearly observed both in the (La0.6Sr0.4)0.95Co0.2Fe0.8O3−δ layer and at the interface with electrolyte. X-ray photoelectron spectroscopy (XPS) indicates that this Sr-rich phase corresponded to SrCO3. These different phenomena led to a chemical degradation of materials and interfaces, explaining the decrease in electrochemical performance.
Old Wind Farm Life Extension vs. Full Repowering: A Review of Economic Issues and a Stochastic Application for Spain
Authors: Abadie Luis M. , Goicoechea Nestor
Published: June 2021

The installation of wind power technology is growing steadily and the trend can be expected to continue if the objectives proposed by the European Commission are to be achieved. In some countries a considerable percentage of installed wind power capacity is near the end of its useful lifetime. In the case of Spain, the figure is 50% within five years. Over the last 20 years, wind energy technology has evolved considerably and the expected capacity factor has improved, thus increasing annual energy production, and capital expenditure and operational expenditure have decreased substantially. This paper studies the optimal decision under uncertainty between life extension and full repowering for a generic wind farm installed in the Iberian Peninsula when the future hourly electricity prices and the capacity factor evolve stochastically and seasonally. The results show that in economic terms, full repowering is the best option, with a net present value of €702,093 per MW installed, while reblading is the second best option. The methodology can be transferred to other specific wind farms in different electricity markets and can be used to develop national wind energy policy recommendations to achieve projected shares in the electricity mix.
Using the Multi-Criteria Model for Optimization of Operational Routes of Thermal Power Plants
Authors: Moraes Cayo Cid de França , Pinheiro Plácido Rogério , Rolim Ivo Goncalves , Costa Jose Luis da Silva , Junior Marcos da Silva Elias , Andrade Sidney Jose Meireles De
Published: June 2021

The constant problems evidenced in the Brazilian hydrological scenario, where the source of hydraulic potential corresponds to about 63.9% of the energy matrix, coupled with the exponential growth in the supply of renewable energy, corroborates the importance of thermal power generation as the basis of Brazilian’s energy matrix. With the operation of thermal power plants, which characteristically involve a large number of systems, subsystems, and auxiliary equipment, there is a high demand for the use of methodologies for monitoring and controlling processes, analyzing failures, and implementing improvements and actions that increase the reliability and, consequently, reduce the failure rate. In this context, decision-making about prioritizing criticality for operational monitoring of an asset’s components, from the perspective of operation and maintenance planning and based on reliability-centered maintenance (RCM) concepts, can be considered a complex task. Given this, the research seeks to demonstrate how the “Measuring Attractiveness by a Category Based Evaluation Technique (MACBETH)” method can be applied to the development of a multiple criteria model to support decision making in ordering the criticality of systems in operational inspection routes of thermal power plants, to propose new routines execution methodologies, aiming at increasing the productivity of the operation team. According to the results of the judgment matrix, the ordering proposal allowed a strategy for the execution of the current operational routes, redefining criticality, periodicity, routing, and applied resources, maintaining the reliability of the plant. It limits itself to assessing the criticality of the subsystems and monitored points belonging to the boiler system. These are typical of thermoelectric plants classified within the Rankine thermodynamic cycle and represent a significant part of the evaluation proposal.
Functional and Operational Requirements to Be Fulfilled by Conical Picks Regarding Their Wear Rate and Investment Costs
Authors: Krauze Krzysztof , Mucha Kamil , Wydro Tomasz , Pieczora Edward
Published: June 2021

User’s choices of conical picks currently involve specifying their material and geometric parameters (functional requirements), incorporating the place and conditions of their work (operational requirements). The selection is usually made based on solely one criterion, which is the price. Thus, at the stage of both purchase and operation, the quality of picks, as well as their matching for a specific machine, are not assessed. The problem of defining whether the producer has fulfilled the user’s requirements arises only when the user questions the picks’ quality. Analysis of this problem has resulted in developing assumptions, concepts and research procedures based on the cutting process requirements. The procedure allows conducting tests to determine the geometric parameters of a pick, the type of material of the pick body and WC-Co insert, as well as the pick wear rate (intensity). The C2 index describes the wear rate (intensity)—the smaller its value, the slower the pick’s wear. Laboratory tests were carried out at the AGH University of Science and Technology in Kraków, Poland. Following the developed method and procedure, the quality of picks was precisely and unambiguously assessed. The C2 index, apart from testing the quality of picks, was also used to forecast their wear. Based on the C2 index, a method is proposed to estimate the wear rate of conical picks provided by different manufacturers and determine the acceptable unit price and operating costs. Thus, it is possible and reasonable to precisely define the investment requirements and appropriately select the pick. Relevant tests were carried out for eight different types of conical picks used in roadheaders, longwall shearers and shaft-boring roadheaders.
Characterizing Industrial and Artisanal Fishing Vessel Catch Composition Using Environmental DNA and Satellite-Based Tracking Data
Authors: Willette Demian A. , Navarrete-Forero Gabriela , Gold Zachary , Lizano Apollo Marco D. , Gonzalez-Smith Leonardo , Sotil Giovanna
Published: June 2021

The decline in wild-caught fisheries paired with increasing global seafood demand is pushing the need for seafood sustainability to the forefront of national and regional priorities. Validation of species identity is a crucial early step, yet conventional monitoring and surveillance tools are limited in their effectiveness because they are extremely time-consuming and require expertise in fish identification. DNA barcoding methods are a versatile tool for the genetic monitoring of wildlife products; however, they are also limited by requiring individual tissue samples from target specimens which may not always be possible given the speed and scale of seafood operations. To circumvent the need to individually sample organisms, we pilot an approach that uses forensic environmental DNA (eDNA) metabarcoding to profile fish species composition from the meltwater in fish holds on industrial and artisanal fishing vessels in Ecuador. Fish identified genetically as present were compared to target species reported by each vessel’s crew. Additionally, we contrasted the geographic range of identified species against the satellite-based fishing route data of industrial vessels to determine if identified species could be reasonably expected in the catch.
Suitability Analysis of 17 Probiotic Type Strains of Lactic Acid Bacteria as Starter for Kimchi Fermentation
Authors: Seo Hee , Bae Jae-Han , Kim Gayun , Kim Seul-Ah , Ryu Byung Hee , Han Nam Soo
Published: June 2021

The use of probiotic starters can improve the sensory and health-promoting properties of fermented foods. This study aimed to evaluate the suitability of probiotic lactic acid bacteria (LAB) as a starter for kimchi fermentation. Seventeen probiotic type strains were tested for their growth rates, volatile aroma compounds, metabolites, and sensory characteristics of kimchi, and their characteristics were compared to those of Leuconostoc (Le.) mesenteroides DRC 1506, a commercial kimchi starter. Among the tested strains, Limosilactobacillus fermentum, Limosilactobacillus reuteri, Lacticaseibacillus rhamnosus, Lacticaseibacillus paracasei, and Ligilactobacillus salivarius exhibited high or moderate growth rates in simulated kimchi juice (SKJ) at 37 °C and 15 °C. When these five strains were inoculated in kimchi and metabolite profiles were analyzed during fermentation using GC/MS and 1H-NMR, data from the principal component analysis (PCA) showed that L. fermentum and L. reuteri were highly correlated with Le. mesenteroides in concentrations of sugar, mannitol, lactate, acetate, and total volatile compounds. Sensory test results also indicated that these three strains showed similar sensory preferences. In conclusion, L. fermentum and L. reuteri can be considered potential candidates as probiotic starters or cocultures to develop health-promoting kimchi products.
The Effect of Scuba Diving on Microleakage of a Class II Composite Restoration: An In-Vitro Study
Authors: Shahnavazi Maryam , Salari Behzad , Fekrazad Reza
Published: June 2021

Persistent pressure change is a common phenomenon within scuba diving with various medical and dental sign side effects. This study evaluates the effect of simulated pressure change due to scuba diving on the microleakage of class II composite restoration. In our methodology, a total number of 150 intact bicuspids are divided into two main groups (A and B), and prepared for a class II composite restoration. The samples of each main group are divided into five subgroups to be prepared with different liners. Then samples are restored with the same resin composite material. The teeth in group A are thermocycled under the normal atmospheric pressure, while group B are thermocycled under simulated scuba diving conditions. The gingival microleakage is assessed based on dye penetration. The group B teeth show a significantly higher microleakage score than their equivalents in group A (p < 0.05). The subgroups without a liner have a higher microleakage score than the other subgroups (p < 0.05). The flowable composite shows the leased leakage scores followed by Nano ionomer, Resin Modified Glass Iononomer, GIOMER, and linerless groups (p < 0.05). Scuba diving could increase the risk of microleakage development beneath class II, a composite restoration.
Assessing the Knowledge, Attitudes and Practices of COVID-19 among Quarantine Hotel Workers in China
Authors: Teng Yi-Man , Wu Kun-Shan , Wang Wen-Cheng , Xu Dan
Published: June 2021

During the pandemic, quarantine hotel workers face a higher risk of infection while they host quarantine guests from overseas. This study’s aim is to gain an understanding of the knowledge, attitudes, and practices (KAP) of quarantine hotel workers in China. A total of 170 participants took part in a cross-sectional survey to assess the KAP of quarantine hotel workers in China, during the COVID-19 pandemic. The chi-square test, independent t-test, one-way analysis of variance (ANOVA), descriptive analysis, and binary logistic regression were used to examine the sociodemographic factors associated with KAP levels during the COVID-19 pandemic. The results show that 62.41% have good knowledge, 94.7% have a positive attitude towards COVID-19, but only 78.2% have good practices. Most quarantine hotel workers (95.3%) are confident that COVID-19 will be successfully controlled and that China is handling the COVID-19 crisis well (98.8%). Most quarantine hotel workers are also taking personal precautions, such as avoiding crowds (80.6%) and wearing facemasks (97.6%). The results evidence that quarantine hotel workers in China have acquired the necessary knowledge, positive attitudes and proactive practices in response to the COVID-19 pandemic. The results of this study can provide a reference for quarantine hotel workers and their targeted education and intervention.
Review of Pharmacotherapy for Tinnitus
Authors: Kim Sang Hoon , Kim Dokyoung , Lee Jae-Min , Lee Sun Kyu , Kang Hee Jin , Yeo Seung Geun
Published: June 2021

Various medications are currently used in the treatment of tinnitus, including anesthetics, antiarrhythmics, anticonvulsants, antidepressants, antihistamines, antipsychotics, anxiolytics, calcium channel blockers, cholinergic antagonists, NMDA antagonists, muscle relaxants, vasodilators, and vitamins. To date, however, no medications have been specifically approved to treat tinnitus by the US Food and Drug Administration (FDA). In addition, medicines used to treat other diseases, as well as foods and other ingested materials, can result in unwanted tinnitus. These include alcohol, antineoplastic chemotherapeutic agents and heavy metals, antimetabolites, antitumor agents, antibiotics, caffeine, cocaine, marijuana, nonnarcotic analgesics and antipyretics, ototoxic antibiotics and diuretics, oral contraceptives, quinine and chloroquine, and salicylates. This review, therefore, describes the medications currently used to treat tinnitus, including their mechanisms of action, therapeutic effects, dosages, and side-effects. In addition, this review describes the medications, foods, and other ingested agents that can induce unwanted tinnitus, as well as their mechanisms of action.
A Novel Educational Smartphone Application for Cognitively Healthy Seniors: A Pilot Study
Authors: Klimova Blanka , Sanda Lukas
Published: June 2021

Modern technologies surround people every day, including seniors. The aim of this pilot study was to create a maximally user-friendly mobile application in order to meet older users’ individual needs. The research sample consisted of 13 older individuals at the age of 55+ years with a mean age of 67 years, living in the Czech Republic. The key assessment tools of this pilot study were the developed application and usability testing. The findings confirmed that the newly developed mobile application for teaching English met the needs of cognitively healthy seniors, and was acceptable and feasible. In addition, it indicated what technical (e.g., visual interface or easy navigation) and pedagogical (e.g., an instructional manual or adjusting to seniors’ learning pace or clear instructions) aspects should be strictly followed when designing such an educational smartphone application. In addition, the authors of this pilot study provide several implications for pedagogical practice. Further research should include more empirical studies aimed at the exploration of educational mobile applications for older generation groups with respect to meeting their individual needs in order to enhance their overall well-being. However, such studies are, nowadays, very rare.
Evaluation of the Diagnostic Accuracy of the Interview and Physical Examination in the Diagnosis of Endometriosis as the Cause of Chronic Pelvic Pain
Authors: Nawrocka-Rutkowska Jolanta , Szydłowska Iwona , Rył Aleksandra , Ciećwież Sylwester , Ptak Magdalena , Starczewski Andrzej
Published: June 2021

Background: Chronic pelvic pain affects approximately 15% of reproductive age women. It is mainly caused by adhesions (20–40%). Despite CPP being the main symptom of endometriosis, the disease is confirmed by laparoscopy only in 12–18% of cases. The aim of this study was to evaluate the results of laparoscopy in women with CCP and to assess the sensitivity and specificity of elements of an interview and clinical examination. Materials and methods: The study included 148 women with CPP. Each patient underwent laparoscopy. In laparoscopy, the presence of endometriosis and/or peritoneal adhesions was confirmed. Then, the sensitivity and specificity and the positive and negative predictive value of endometriosis symptoms or abnormalities in the gynecological examination were statistically calculated. Results: After previous surgery, adhesions were found in almost half (47%) of patients. In patients without a history of surgery, adhesions were diagnosed in 6.34% of patients. Endometriosis without coexisting adhesions was more often diagnosed in women without previous surgery (34.9%), compared to 10.58% in the group with a history of surgery (p < 0.05). Conclusions: Intraperitoneal adhesions are most common in women after pelvic surgery and with chronic ailments. The best results for sensitivity, specificity, positive predictive value, and negative predictive value in the diagnosis of endometriosis are found in women with irregular menstruations during which the pain increases. Laparoscopy still remains the primary diagnostic and therapeutic method for these women.
Assessing Nurses’ Satisfaction with Continuity of Care and the Case Management Model as an Indicator of Quality of Care in Spain
Authors: Reig-Garcia Gloria , Suñer-Soler Rosa , Mantas-Jiménez Susana , Bonmatí-Tomas Anna , Malagón-Aguilera Maria Carmen , Bosch-Farré Cristina , Gelabert-Viella Sandra , Juvinyà-Canal Dolors
Published: June 2021

Chronic diseases are treated and cared for in different healthcare settings. Continuity of care and the case management model facilitate the integration of processes and care levels. However, there is little evidence regarding the satisfaction of nurses with this model. The purpose of this study was to examine nurses’ satisfaction with continuity of care and the case management model. A cross-sectional study was conducted. An ad hoc questionnaire was administrated to 437 Spanish nurses from the three health care settings that responded. This included items on socio-demographics, employment relationship, and satisfaction with continuity of care and case management. Descriptive analysis and linear regression models were performed. In total, 96.1% of the nurses expressed a high level of satisfaction with continuity of care and 80.7% with the case management model. Nurses in a primary care setting reported the greatest satisfaction with the case management model (B = 0.146, 95% CI = 0.139–0.694, p = 0.003). The nurses’ higher perception of patient satisfaction was associated with greater satisfaction with continuity of care (B = 0.466, 95% CI = −0.367–0.533, p < 0.000). Nurses identified the case management model as an optimal facilitator of continuity of care. While satisfaction with continuity is high, strategies are needed to improve it in primary care centers and aged care homes.
Effect of Vermicompost Amendment on the Accumulation and Chemical Forms of Trace Metals in Leafy Vegetables Grown in Contaminated Soils
Authors: Yen Yu-Shan , Chen Kuei-San , Yang Hsin-Yi , Lai Hung-Yu
Published: June 2021

(1) Background: Trace metal (TM) contamination of farmland soil in Taiwan occurs because factories dump wastewater into irrigation ditches. Since vermicompost affects the bioavailability of TMs, the objective of this study was to evaluate its effects on the accumulation of growth of TMs in leafy vegetables. (2) Methods: Two TM-contaminated soils and different types of pak choi and lettuce were used and amended with vermicompost. Besides soil properties, the study assessed vermicompost’s influence on the growth, accumulation, and chemical forms of TMs and on the health risks posed by oral intake. (3) Results: Vermicompost could increase the content of soil organic matter, available phosphorus, exchangeable magnesium, and exchangeable potassium, thus promoting the growth of leafy vegetables. The accumulation of four TMs in crops under vermicompost was reduced compared to the control, especially for the concentration of cadmium, which decreased by 60–75%. The vermicompost’s influence on changing the chemical form of TMs depended on the TM concentrations, type of TM, and crop species; moreover, blanching effectively reduced the concentrations of TMs in high-mobility chemical forms. Although vermicompost mostly reduced the amount of cadmium consumed via oral intake, cadmium still posed the highest health risk compared to the other three TMs.
Secular Trend, Seasonal Variation, Epidemiological Pattern, and Outcome of Traumatic Head Injuries Due to Road Traffic Accidents in Aseer, Saudi Arabia
Authors: Algahtany Mubarak Ali
Published: June 2021

Road traffic accidents (RTAs) are a leading cause of traumatic head injury (THI) and are regarded as a public health problem in Saudi Arabia. This hospital-based retrospective study aims to provide data on the frequency, type, and distribution of RTA-related THIs over the past decade; demonstrate their time trend and seasonality; and decipher age and sex differences in RTA-related THIs and their outcome. The results showed a decline in the number of RTA-related THIs between 2010 and 2019. The patients had a mean age of 26.16 ± 16.27 years, and the male-to-female ratio was 10.8:1. Head injury with multiple lesions was the most common diagnosis, followed by cerebral contusion and skull fracture (32.1%, 12.9%, and 11.2%, respectively). Subdural hematoma (SDH) and skull fracture were significantly more common in patients aged ≥60 years (standard residual > 1.96), and significantly less common in those aged ≤17 years (standard residual < 1.96), compared to other age groups. Males experienced significantly more SDHs than females (standard residual = −2.8, p = 0.029). The length of hospital stay was positively correlated with age (Spearman’s rho = 0.057, p = 0.046). No seasonal variation was found.
Associations between Psychosocial Variables, Availability of Physical Activity Resources in Neighborhood Environment, and Out-of-School Physical Activity among Chinese Adolescents
Authors: Qiu Nan , Gao Xiaoli , Zhang Xinge , Fu Jialin , Wang Yechuang , Li Rui
Published: June 2021

This study aimed to evaluate the relationship between psychosocial variables (peer support, parental support, autonomous motivation, and controlled motivation), availability of physical activity resources in a neighborhood environment, and out-of-school moderate to vigorous physical activity (MVPA) among Chinese adolescents. The questionnaire of Family Life, Activity, Sun, Health, and Eating (FLASHE) Study was used to collect information on demographics, socioeconomic status, psychosocial variables, available physical activity resources in the neighborhood environment, and minutes of out-of-school MVPA. ANOVA analysis and multiple regression analysis were performed. The mean age of the 3833 adolescents included in our analysis was 14.7 years old (SD = 1.7). Peer support (b = 9.35, 95% CI: 7.55–11.15), autonomous motivation (b = 6.46, 95% CI: 4.09–8.82), parental support (b = 3.90, 95% CI: 1.75–6.07), and availability of physical activity resources in neighborhood environment (b = 3.18, 95% CI: 1.99–4.36) were significantly associated with out-of-school MVPA (p < 0.05). Controlled motivation was insignificantly related to minutes of out-of-school MVPA. Boys spent more time on out-of-school MVPA than girls (p < 0.001) and had a high level of peer support, parental support, and motivation (p < 0.05). Our findings suggest that interventions targeting the out-of-school MVPA among Chinese adolescents should focus on the psychosocial variables and neighborhood environment.
Effectiveness of Text Messaging as an Incentive to Maintain Physical Activity after Cardiac Rehabilitation: A Randomized Controlled Pilot Study
Authors: Foccardi Giulia , Vecchiato Marco , Neunhaeuserer Daniel , Mezzaro Michele , Quinto Giulia , Battista Francesca , Duregon Federica , Carlon Roberto , Ermolao Andrea
Published: June 2021

Although the efficacy of cardiac rehabilitation (CR) is proven, the need to improve patients’ adherence has emerged. There are only a few studies that have investigated the effect of sending text messages after a CR period to stimulate subjects’ ongoing engagement in regular physical activity (PA). A randomized controlled pilot trial was conducted after CR, sending a daily PA text message reminder to an intervention group (IG), which was compared with a usual care control group (CG) during three months of follow-up. Thirty-two subjects were assessed pre- and post-study intervention with GPAQ, submaximal iso-watt exercise testing, a 30 s sit-to-stand test, a bilateral arm curl test, and a final survey on a seven-point Likert scale. A statistically significant difference in the increase of moderate PA time (Δ 244.7 (95% CI 189.1, 300.4) minutes, p < 0.001) and in the reduction of sedentary behavior time (Δ −77.5 (95% CI 104.9, −50.1) minutes, p = 0.004) was shown when the IG was compared with the CG. This was associated with an improvement in heart rate, blood pressure, and patients’ Borg rating on the category ratio scale 10 (CR10) in iso-watt exercise testing (all p < 0.05). Furthermore, only the IG did not show a worsening of the strength parameters in the follow-up leading to a change of the 30 s sit-to-stand test with a difference of +2.2 (95% CI 1.23, 3.17) repetitions compared to CG (p = 0.03). The telemedical intervention has been appreciated by the IG, whose willingness to continue with regular PA emerged to be superior compared to the CG. Text messages are an effective and inexpensive adjuvant after phase 2 CR that improves adherence to regular PA. Further studies are needed to confirm these results in a larger patient population and in the long term.
Slut Shaming in Adolescence: A Violence against Girls and Its Impact on Their Health
Authors: Goblet Margot , Glowacz Fabienne
Published: June 2021

Slut shaming is defined as the stigmatization of an individual based on his or her appearance, sexual availability, and actual or perceived sexual behavior. It can take place in physical or virtual spaces. The present study questions the impact of this form of sexism in virtual spaces on girls and interrogates the interaction between the values that girls integrate through their life experiences, especially in the family sphere, and slut shaming victimization. We conducted a paper-pencil questionnaire with 605 girls between the ages of 10 and 18 (average age: 15.18 years). Our data confirm the impact of slut shaming on the physical and psychological well-being of young girls as early as adolescence. Second, mediation analyses provide insights into the revictimization and Poly-victimization processes, from childhood adverse experiences to sexist victimization in virtual spaces and their combined impact on the physical and psychic health of girls. Finally, we address prevention strategies and the involvement of socializing institutions in the deconstruction of gender stereotypes.
Women’s Usage Behavior and Perceived Usefulness with Using a Mobile Health Application for Gestational Diabetes Mellitus: Mixed-Methods Study
Authors: Surendran Shilpa , Lim Chang Siang , Koh Gerald Choon Huat , Yew Tong Wei , Tai E Shyong , Foong Pin Sym
Published: June 2021

The prevalence of gestational diabetes mellitus (GDM) is increasing, and only a few mobile health (mHealth) applications are specifically designed to manage GDM. In this mixed-methods study, a follow-up study of a randomized controlled trial (RCT) analyzed a largely automated mHealth application-based lifestyle coaching program to (a) measure the application’s usage behavior and (b) explore users’ perceptions of its usefulness in GDM management. Quantitative data were collected from the 170 application users who had participated in the intervention arm of the RCT. Semi-structured interviews (n = 14) captured users’ experiences when using the application. Data were collected from June 2019 to January 2020. Quantitative data were analyzed descriptively, and interviews were analyzed thematically. Only 57/170 users (34%) logged at least one meal, and only 35 meals on average were logged for eight weeks because of the incorrectly worded food items and limited food database. On the contrary, an average of 1.85 (SD = 1.60) weight values were logged per week since the weight tracking component was easy to use. Many users (6/14 (43%)) mentioned that the automatic coach messages created an immediate sense of self-awareness in food choices and motivated behavior. The findings suggest that for GDM management, a largely automated mHealth application has the potential to promote self-awareness of healthy lifestyle choices, reducing the need for intensive human resources. Additionally, several gaps in the application’s design were identified which need to be addressed in future works.
Adolescents’ Perceptions of Their Problematic Use of ICT: Relationship with Study Time and Academic Performance
Authors: Díaz-López Adoración , Mirete-Ruiz Ana Belén , Maquilón-Sánchez Javier
Published: June 2021

Today, the use of Information and Communication Technologies (ICT) is part of the daily lives of adolescents. However, its widespread use in all areas, the vulnerable condition of adolescents and the imminent consequences of problematic use are awakening a growing social and educational concern. With the purpose of looking into this problem, the following research aims are formulated: (1) Analyse the perception of adolescents about their academic performance and the interference of ICT in their development; (2) Describe the frequency of use of ICT and its influence on study time and grades; and (3) Analyse the relationship between family supervision of ICT and academic performance. The representative sample consisted of 1101 adolescents from 10 educational centers in the Southeast of Spain. Descriptive statistics, contingency tables, Chi Square, Cramer’s V and Linear Regression were calculated. The results show that more than 50% of the students believe that they would spend more time studying if they did not have continuous access to technologies. Likewise, 20% of the students identify ICT as responsible for the decline in their academic performance. Statistically significant relationships were found between time limitations for Internet access and academic performance. It is therefore concluded that the problematic use of ICT in adolescence is a phenomenon that demands intervention, and the training of parents and adolescents in the responsible use of ICT is urged.
Heparan Sulfate Proteoglycans in Viral Infection and Treatment: A Special Focus on SARS-CoV-2
Authors: De Pasquale Valeria , Quiccione Miriam Shasa , Tafuri Simona , Avallone Luigi , Pavone Luigi Michele
Published: June 2021

Heparan sulfate proteoglycans (HSPGs) encompass a group of glycoproteins composed of unbranched negatively charged heparan sulfate (HS) chains covalently attached to a core protein. The complex HSPG biosynthetic machinery generates an extraordinary structural variety of HS chains that enable them to bind a plethora of ligands, including growth factors, morphogens, cytokines, chemokines, enzymes, matrix proteins, and bacterial and viral pathogens. These interactions translate into key regulatory activity of HSPGs on a wide range of cellular processes such as receptor activation and signaling, cytoskeleton assembly, extracellular matrix remodeling, endocytosis, cell-cell crosstalk, and others. Due to their ubiquitous expression within tissues and their large functional repertoire, HSPGs are involved in many physiopathological processes; thus, they have emerged as valuable targets for the therapy of many human diseases. Among their functions, HSPGs assist many viruses in invading host cells at various steps of their life cycle. Viruses utilize HSPGs for the attachment to the host cell, internalization, intracellular trafficking, egress, and spread. Recently, HSPG involvement in the pathogenesis of SARS-CoV-2 infection has been established. Here, we summarize the current knowledge on the molecular mechanisms underlying HSPG/SARS-CoV-2 interaction and downstream effects, and we provide an overview of the HSPG-based therapeutic strategies that could be used to combat such a fearsome virus.
Elevation of Chemosensitivity of Lung Adenocarcinoma A549 Spheroid Cells by Claudin-2 Knockdown through Activation of Glucose Transport and Inhibition of Nrf2 Signal
Authors: Ito Ayaka , Nasako Haruka , Akizuki Risa , Takashina Yui , Eguchi Hiroaki , Matsunaga Toshiyuki , Yoshino Yuta , Endo Satoshi , Ikari Akira
Published: June 2021

Claudin-2 (CLDN2), a tight junctional protein, is involved in the chemoresistance in a three-dimensional spheroid culture model of human lung adenocarcinoma A549 cells. However, the mechanism has not been fully clarified. We found that the knockdown of CLDN2 expression by siRNA in the spheroid reduces the expression of glucose transporters and metabolic enzymes. In a two-dimensional culture model, the expression of these proteins was increased by glucose deprivation or fasentin, an inhibitor of glucose transporter. In addition, the expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and antioxidant enzymes including heme oxygenase-1, NAD(P)H:quinone oxidoreductase-1, and a glutamate–cysteine ligase modifier subunit were increased by fasentin. The fluorescence intensities of JC-1, a probe of mitochondrial membrane potential, and MitoROS 580, a probe of mitochondrial superoxide production, were increased by fasentin. These results suggest that mitochondrial production of reactive oxygen species is increased by glucose deficiency. The knockdown of CLDN2 enhanced the flux of 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose (2-NBDG), a fluorescent deoxyglucose derivative, in a transwell assay, and the accumulation of glucose and 2-NBDG in spheroid cells. The expression of Nrf2 was decreased by CLDN2 knockdown, which was inhibited by fasentin and sulforaphane, a typical Nrf2 activator, in spheroid cells. The sensitivity of spheroid cells to doxorubicin, an anthracycline antitumor antibiotic, was enhanced by CLDN2 knockdown, which was inhibited by fasentin and sulforaphane. We suggest that CLDN2 induces chemoresistance in spheroid cells mediated through the inhibition of glucose transport and activation of the Nrf2 signal.
Induction of Tertiary Phase Epileptiform Discharges after Postasphyxial Infusion of a Toll-Like Receptor 7 Agonist in Preterm Fetal Sheep
Authors: Cho Kenta H.T. , Fraser Mhoyra , Xu Bing , Dean Justin M. , Gunn Alistair J. , Bennet Laura
Published: June 2021

Background: Toll-like receptor (TLR) agonists are key immunomodulatory factors that can markedly ameliorate or exacerbate hypoxic–ischemic brain injury. We recently demonstrated that central infusion of the TLR7 agonist Gardiquimod (GDQ) following asphyxia was highly neuroprotective after 3 days but not 7 days of recovery. We hypothesize that this apparent transient neuroprotection is associated with modulation of seizure-genic processes and hemodynamic control. Methods: Fetuses received sham asphyxia or asphyxia induced by umbilical cord occlusion (20.9 ± 0.5 min) and were monitored continuously for 7 days. GDQ 3.34 mg or vehicle were infused intracerebroventricularly from 1 to 4 h after asphyxia. Results: GDQ infusion was associated with sustained moderate hypertension that resolved after 72 h recovery. Electrophysiologically, GDQ infusion was associated with reduced number and burden of postasphyxial seizures in the first 18 h of recovery (p < 0.05). Subsequently, GDQ was associated with induction of slow rhythmic epileptiform discharges (EDs) from 72 to 96 h of recovery (p < 0.05 vs asphyxia + vehicle). The total burden of EDs was associated with reduced numbers of neurons in the caudate nucleus (r2 = 0.61, p < 0.05) and CA1/2 hippocampal region (r2 = 0.66, p < 0.05). Conclusion: These data demonstrate that TLR7 activation by GDQ modulated blood pressure and suppressed seizures in the early phase of postasphyxial recovery, with subsequent prolonged induction of epileptiform activity. Speculatively, this may reflect delayed loss of early protection or contribute to differential neuronal survival in subcortical regions.
The Intestinal Microbiota: Impacts of Antibiotics Therapy, Colonization Resistance, and Diseases
Authors: Shah Taif , Baloch Zulqarnain , Shah Zahir , Cui Xiuming , Xia Xueshan
Published: June 2021

Trillions of microbes exist in the human body, particularly the gastrointestinal tract, coevolved with the host in a mutually beneficial relationship. The main role of the intestinal microbiome is the fermentation of non-digestible substrates and increased growth of beneficial microbes that produce key antimicrobial metabolites such as short-chain fatty acids, etc., to inhibit the growth of pathogenic microbes besides other functions. Intestinal microbiota can prevent pathogen colonization through the mechanism of colonization resistance. A wide range of resistomes are present in both beneficial and pathogenic microbes. Giving antibiotic exposure to the intestinal microbiome (both beneficial and hostile) can trigger a resistome response, affecting colonization resistance. The following review provides a mechanistic overview of the intestinal microbiome and the impacts of antibiotic therapy on pathogen colonization and diseases. Further, we also discuss the epidemiology of immunocompromised patients who are at high risk for nosocomial infections, colonization and decolonization of multi-drug resistant organisms in the intestine, and the direct and indirect mechanisms that govern colonization resistance to the pathogens.
Alleviation of Memory Deficit by Bergenin via the Regulation of Reelin and Nrf-2/NF-κB Pathway in Transgenic Mouse Model
Authors: Shal Bushra , Khan Adnan , Khan Ashraf Ullah , Ullah Rahim , Ali Gowhar , Islam Salman Ul , Haq Ihsan ul , Ali Hussain , Seo Eun-Kyoung , Khan Salman
Published: June 2021

The present study aims to determine the neuroprotective effect of Bergenin against spatial memory deficit associated with neurodegeneration. Preliminarily, the protective effect of Bergenin was observed against H2O2-induced oxidative stress in HT-22 and PC-12 cells. Further studies were performed in 5xFAD Tg mouse model by administering Bergenin (1, 30 and 60 mg/kg; orally), whereas Bergenin (60 mg/kg) significantly attenuated the memory deficit observed in the Y-maze and Morris water maze (MWM) test. Fourier transform-infrared (FT-IR) spectroscopy displayed restoration of lipids, proteins and their derivatives compared to the 5xFAD Tg mice group. The differential scanning calorimeter (DSC) suggested an absence of amyloid beta (Aβ) aggregation in Bergenin-treated mice. The immunohistochemistry (IHC) analysis suggested the neuroprotective effect of Bergenin by increasing Reelin signaling (Reelin/Dab-1) and attenuated Aβ (1–42) aggregation in hippocampal regions of mouse brains. Furthermore, IHC and western blot results suggested antioxidant (Keap-1/Nrf-2/HO-1), anti-inflammatory (TLR-4/NF-kB) and anti-apoptotic (Bcl-2/Bax/Caspase-3) effect of Bergenin. Moreover, a decrease in Annexin V/PI-stained hippocampal cells suggested its effect against neurodegeneration. The histopathological changes were reversed significantly by Bergenin. In addition, a remarkable increase in antioxidant level with suppression of pro-inflammatory cytokines, oxidative stress and nitric oxide production were observed in specific regions of the mouse brains.
SOS2 Comes to the Fore: Differential Functionalities in Physiology and Pathology
Authors: Baltanás Fernando C. , García-Navas Rósula , Santos Eugenio
Published: June 2021

The SOS family of Ras-GEFs encompasses two highly homologous and widely expressed members, SOS1 and SOS2. Despite their similar structures and expression patterns, early studies of constitutive KO mice showing that SOS1-KO mutants were embryonic lethal while SOS2-KO mice were viable led to initially viewing SOS1 as the main Ras-GEF linking external stimuli to downstream RAS signaling, while obviating the functional significance of SOS2. Subsequently, different genetic and/or pharmacological ablation tools defined more precisely the functional specificity/redundancy of the SOS1/2 GEFs. Interestingly, the defective phenotypes observed in concomitantly ablated SOS1/2-DKO contexts are frequently much stronger than in single SOS1-KO scenarios and undetectable in single SOS2-KO cells, demonstrating functional redundancy between them and suggesting an ancillary role of SOS2 in the absence of SOS1. Preferential SOS1 role was also demonstrated in different RASopathies and tumors. Conversely, specific SOS2 functions, including a critical role in regulation of the RAS–PI3K/AKT signaling axis in keratinocytes and KRAS-driven tumor lines or in control of epidermal stem cell homeostasis, were also reported. Specific SOS2 mutations were also identified in some RASopathies and cancer forms. The relevance/specificity of the newly uncovered functional roles suggests that SOS2 should join SOS1 for consideration as a relevant biomarker/therapy target.