bluuebunny/crossref_metadata_2025 huggingface dataset:
Symmetries of Scan Patterns of Laser Scanners with Rotational Risley Prisms
Authors: Dimb Alexandru-Lucian , Duma Virgil-Florin
Published: January 2023

Laser scanners with rotational Risley prisms produce scan patterns that can be classified as a type of rhodonea curve, because of their symmetry. The present study builds upon the novel, graphical method that we have developed to simulate and analyze such patterns using a commercially available mechanical design program, CATIA V5R20 (Dassault Systems, Paris, France). This graphical method has both the advantage of simplicity (regarding analytical methods) and of generating exact scan patterns (in contrast to approximate methods). The aim of this work is to utilize this method to study symmetry properties of scan patterns produced by a pair of rotational Risley prisms. A multi-parameter analysis is performed, considering the characteristic parameters of the approached scanner: refractive indexes and angles of the prisms, as well as their rotational velocities. Furthermore, Marshall’s parameters are considered: ratios of the rotational velocities and of the prism angles. Because the symmetries are identical for scan patterns generated by all the four possible configurations of a pair of prisms (and only their dimensions are different), the shape of these patterns is studied only for one of these configurations. Therefore, without losing generality, the symmetries of the patterns produced by such scanners are determined, as well as some of their characteristic dimensions. Rules-of-thumb were obtained to be able to generate patterns that are appropriate for a specific application. Moreover, we demonstrated that the existing symmetries simplify the process of obtaining the scan patterns by identifying repetitive parts (defined as structures of symmetry) of a pattern.
RNA Structure in the 5′ Untranslated Region of Enterovirus D68 Strains with Differing Neurovirulence Phenotypes
Authors: Eastman Connor , Tapprich William E.
Published: January 2023

Enterovirus-D68 (EV-D68) is a positive-sense single-stranded RNA virus within the family Picornaviridae. EV-D68 was initially considered a respiratory virus that primarily affected children. However, in 2014, EV-D68 outbreaks occurred causing the expected increase in respiratory illness cases, but also an increase in acute flaccid myelitis cases (AFM). Sequencing of 2014 outbreak isolates revealed variations in the 5′ UTR of the genome compared to the historical Fermon strain. The structure of the 5′ UTR RNA contributes to enterovirus virulence, including neurovirulence in poliovirus, and could contribute to neurovirulence in contemporary EV-D68 strains. In this study, the secondary and tertiary structures of 5′ UTR RNA from the Fermon strain and 2014 isolate KT347251.1 are analyzed and compared. Secondary structures were determined using SHAPE-MaP and TurboFold II and tertiary structures were predicted using 3dRNAv2.0. Comparison of RNA structures between the EV-D68 strains shows significant remodeling at the secondary and tertiary levels. Notable secondary structure changes occurred in domains II, IV and V. Shifts in the secondary structure changed the tertiary structure of the individual domains and the orientation of the domains. Our comparative structural models for EV-D68 5′ UTR RNA highlight regions of the molecule that could be targeted for treatment of neurotropic enteroviruses.
Dolutegravir Plus 3TC in Virologically Suppressed PLWHIV: Immunological Outcomes in a Multicenter Retrospective Cohort in Spain during the COVID-19 Pandemic
Authors: Buzón Luis , Dueñas Carlos , Pedrero Roberto , Iribarren Jose , de los Santos Ignacio , Díaz de Santiago Alberto , Morán Miguel , Pousada Guillermo , Moreno Estela , Ferreira Eva , Iglesias Alicia , Martín Cristina , Gómez Julia , Rodríguez Laura , Egido Miguel , Sepulveda María-Antonia , Troya Jesús
Published: January 2023

Dolutegravir (DTG) based dual therapies for treating PLWHIV are a standard of care nowadays. Switching to DTG and lamivudine (3TC) safety and efficacy were proven in TANGO randomized clinical trial. This multicenter retrospective study included 1032 HIV virologically suppressed patients switching to DTG+3TC from 13 Spanish hospitals. DTG+3TC provided high rates of undetectable viral load over 96%, corresponding to 96.6% (889/921) at 24 weeks, 97.5% (743/763) at 48 weeks, and 98.3% (417/425) at 96 weeks. No significant differences are evident when comparing the total population according to sex, presence of comorbidity, or presence of AIDS. The analysis for paired data showed an increase in CD4+ cell count. A statistically significant increase in CD4+ lymphocyte count was found in those without comorbidities in the three-time series analyzed [average increase at 24 weeks: 48.7 (SD: 215.3) vs. 25.8 (SD: 215.5), p-value = 0.050; a mean increase at 48 weeks: 75.1 (SD: 232.9) vs. 42.3 (SD: 255.6), p-value = 0.003; a mean increase at 96 weeks: 120.1 (SD: 205.0) vs. 63.8 (SD:275.3), p-value = 0.003]. In conclusion, our cohort demonstrates that DTG+3TC is an effective treatment strategy for virologically-suppressed PLWHIV independent of age, sex, and HIV stage, as well as a safe and durable strategy.
HPV16 E6 and E7 Oncoproteins Stimulate the Glutamine Pathway Maintaining Cell Proliferation in a SNAT1-Dependent Fashion
Authors: Ortiz-Pedraza Yunuen , Muñoz-Bello J. Omar , Ramos-Chávez Lucio Antonio , Martínez-Ramírez Imelda , Olmedo-Nieva Leslie , Manzo-Merino Joaquín , López-Saavedra Alejandro , Pérez-de la Cruz Verónica , Lizano Marcela
Published: January 2023

Persistent high-risk human papillomavirus infection is the main risk factor for cervical cancer establishment, where the viral oncogenes E6 and E7 promote a cancerous phenotype. Metabolic reprogramming in cancer involves alterations in glutamine metabolism, also named glutaminolysis, to provide energy for supporting cancer processes including migration, proliferation, and production of reactive oxygen species, among others. The aim of this work was to analyze the effect of HPV16 E6 and E7 oncoproteins on the regulation of glutaminolysis and its contribution to cell proliferation. We found that the E6 and E7 oncoproteins exacerbate cell proliferation in a glutamine-dependent manner. Both oncoproteins increased the levels of transporter SNAT1, as well as GLS2 and GS enzymes; E6 also increased LAT1 transporter protein levels, while E7 increased ASCT2 and xCT. Some of these alterations are also regulated at a transcriptional level. Consistently, the amount of SNAT1 protein decreased in Ca Ski cells when E6 and E7 expression was knocked down. In addition, we demonstrated that cell proliferation was partially dependent on SNAT1 in the presence of glutamine. Interestingly, SNAT1 expression was higher in cervical cancer compared with normal cervical cells. The high expression of SNAT1 was associated with poor overall survival of cervical cancer patients. Our results indicate that HPV oncoproteins exacerbate glutaminolysis supporting the malignant phenotype.
Phage–Plant Interactions: A Way Forward toward Sustainable Agriculture
Authors: Ahmed Temoor , Li Bin
Published: January 2023

Agriculture is the most important sector as it provides food to the growing global population [...]
A Glimpse at the Anti-Phage Defenses Landscape in the Foodborne Pathogen Salmonella enterica subsp. enterica serovar Typhimurium
Authors: Woudstra Cedric , Granier Sophie A.
Published: January 2023

Bacteriophages, which specifically infect and kill bacteria, are currently used as additives to control pathogens such as Salmonella in human food (PhageGuard S®) or animal feed (SalmoFREE®, Bafasal®). Indeed, salmonellosis is among the most important zoonotic foodborne illnesses. The presence of anti-phage defenses protecting bacteria against phage infection could impair phage applications aiming at reducing the burden of foodborne pathogens such as Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) to the food industry. In this study, the landscape of S. Typhimurium anti-phage defenses was bioinformatically investigated in publicly available genomes using the webserver PADLOC. The primary anti-phage systems identified in S. Typhimurium use nucleic acid degradation and abortive infection mechanisms. Reference systems were identified on an integrative and conjugative element, a transposon, a putative integrative and mobilizable element, and prophages. Additionally, the mobile genetic elements (MGEs) containing a subset of anti-phage systems were found in the Salmonella enterica species. Lastly, the MGEs alone were also identified in the Enterobacteriaceae family. The presented diversity assessment of the anti-phage defenses and investigation of their dissemination through MGEs in S. Typhimurium constitute a first step towards the design of preventive measures against the spread of phage resistance that may hinder phage applications.
Detection of Hepatitis E Virus Genotype 3 in Feces of Capybaras (Hydrochoeris hydrochaeris) in Brazil
Authors: Cunha Lia , Luchs Adriana , Azevedo Lais S. , Silva Vanessa C. M. , Lemos Marcilio F. , Costa Antonio C. , Compri Adriana P. , França Yasmin , Viana Ellen , Malta Fernanda , Medeiros Roberta S. , Guiducci Raquel , Morillo Simone G. , Gomes-Gouvea Michele S. , Amgarten Deyvid , Pinho João R. R. , Moreira Regina C.
Published: January 2023

Hepatitis E virus (HEV) is an emerging zoonotic pathogen associated with relevant public health issues. The aim of this study was to investigate HEV presence in free-living capybaras inhabiting urban parks in São Paulo state, Brazil. Molecular characterization of HEV positive samples was undertaken to elucidate the genetic diversity of the virus in these animals. A total of 337 fecal samples were screened for HEV using RT-qPCR and further confirmed by conventional nested RT-PCR. HEV genotype and subtype were determined using Sanger and next-generation sequencing. HEV was detected in one specimen (0.3%) and assigned as HEV-3f. The IAL-HEV_921 HEV-3f strain showed a close relationship to European swine, wild boar and human strains (90.7–93.2% nt), suggesting an interspecies transmission. Molecular epidemiology of HEV is poorly investigated in Brazil; subtype 3f has been reported in swine. This is the first report of HEV detected in capybara stool samples worldwide.
Bell’s Palsy and COVID-19 Vaccines: A Systematic Review and Meta-Analysis
Authors: Albakri Khaled , Khaity Abdulrhman , Atwan Hany , Saleh Othman , Al-Hajali Momen , Cadri Shirin , Diab Rehab Adel , Albazee Ebraheem , Negida Ahmed
Published: January 2023

Introduction: Once the COVID-19 pandemic was declared, the world was waiting for the clue that would be able to cut down the progression of the disease. Vaccines play a crucial role in reducing the disease and saving many people worldwide. However, there are several side effects of these vaccines, like pain, fatigue, fever, and neurological defects like Bell’s palsy. In this systematic review, we presented evidence about the occurrence of Bell’s palsy followed by COVID-19 vaccination. Methods: We searched PubMed, SCOPUS, EBSCO, and Web of Science (WOS) from inception till October 2022. The quality assessment was conducted using the Joanna Briggs Institute, National Institute of Health, and Newcastle-Ottawa. The analysis was conducted on SPSS. Results: Thirty-five records were involved in our study. The results of our cases revealed that most of the patients (62.8%) experienced unilateral facial paralysis. Also, the majority of the cases were reported after the first dose, and most cases were after Pfizer, AstraZeneca, and Sputnik V vaccines, respectively. The patients who were treated with corticosteroids, IVIG, and anti-viral drugs, showed marked recovery afterward. Conclusion: The rate ratio of Bell’s palsy after COVID-19 vaccination was 25.3 per 1,000,000. The ratio was higher after the first dose compared to the second dose and was higher among those who took Oxford/AstraZeneca vaccine compared to other vaccines. However, this condition was reported in a small number of cases among a large number of vaccinated people worldwide. It is important to note that the benefits of getting vaccinated far outweigh any potential risks.
Cap-Independent Circular mRNA Translation Efficiency
Authors: Deviatkin Andrei A. , Simonov Ruslan A. , Trutneva Kseniya A. , Maznina Anna A. , Soroka Anastasiia B. , Kogan Anna A. , Feoktistova Sofya G. , Khavina Elena M. , Mityaeva Olga N. , Volchkov Pavel Y.
Published: January 2023

Recently, the mRNA platform has become the method of choice in vaccine development to find new ways to fight infectious diseases. However, this approach has shortcomings, namely that mRNA vaccines require special storage conditions, which makes them less accessible. This instability is due to the fact that the five-prime and three-prime ends of the mRNA are a substrate for the ubiquitous exoribonucleases. To address the problem, circular mRNAs have been proposed for transgene delivery as they lack these ends. Notably, circular RNAs do not have a capped five-prime end, which makes it impossible to initiate translation canonically. In this review, we summarize the current knowledge on cap-independent translation initiation methods and discuss which approaches might be most effective in developing vaccines and other biotechnological products based on circular mRNAs.
Evolution of Tick Vaccinology Highlights Changes in Paradigms in This Research Area
Authors: Estrada-Peña Agustín , Fuente José de la
Published: January 2023

Ticks and tick-borne diseases affect human and animal health worldwide. Although some tick-protective antigens have been identified and characterized, further research is needed for the development and application of effective anti-tick vaccines, which currently are unavailable for human protection. To study the trends and gaps in anti-tick vaccine approaches, herein we used scientometric analysis to evaluate several aspects of tick vaccinology. Co-authorship and citations networks pointed out two main research fronts, one focused on the laboratory protocols driving the recognition of candidate antigens and the other devoted to field experiments of protection against ticks. The analysis demonstrated the prominence of research in European countries on the topic. The scientometric approach allowed the recognition of isolated teams working casually on the topic, the lack of cooperation between middle- and low-income countries, and the need for sustained and integrated research. Most important, we identified a considerable lack of new candidates for vaccine development, as well as the participation of African and Asian countries. These results provide significant insights obtained from bibliographical analysis, suggest the strength and weaknesses in this field of research, and highlight new directions to advance in the development of effective vaccines for the control of tick infestations and tick-borne pathogens.
A Live Attenuated COVID-19 Candidate Vaccine for Children: Protection against SARS-CoV-2 Challenge in Hamsters
Authors: Mehla Rajeev , Kokate Prasad , Bhosale Sarika R. , Vaidya Vivek , Narayanan Shridhar , Shandil Radha. K. , Singh Mayas , Rudramurthy Gudepalya R. , Naveenkumar Chakenahalli N. , Bharathkumar Kumaraswamy , Coleman Rob , Mueller Steffen , Dhere Rajeev M. , Yeolekar Leena R.
Published: January 2023

Children are at risk of infection from severe acute respiratory syndrome coronavirus-2 virus (SARS-CoV-2) resulting in coronavirus disease (COVID-19) and its more severe forms. New-born infants are expected to receive short-term protection from passively transferred maternal antibodies from their mothers who are immunized with first-generation COVID-19 vaccines. Passively transferred antibodies are expected to wane within first 6 months of infant’s life, leaving them vulnerable to COVID-19. Live attenuated vaccines, unlike inactivated or viral-protein-based vaccines, offer broader immune engagement. Given effectiveness of live attenuated vaccines in controlling infectious diseases such as mumps, measles and rubella, we undertook development of a live attenuated COVID-19 vaccine with an aim to vaccinate children beyond 6 months of age. An attenuated vaccine candidate (dCoV), engineered to express sub-optimal codons and deleted polybasic furin cleavage sites in the spike protein of the SARS-CoV-2 WA/1 strain, was developed and tested in hamsters. Hamsters immunized with dCoV via intranasal or intramuscular routes induced high levels of neutralizing antibodies and exhibited complete protection against the SARS-CoV-2 wild-type isolates, i.e., the Wuhan-like (USA-WA1/2020) and Delta variants (B.1.617.2) in a challenge study. In addition, the dCoV formulated with the marketed measles–rubella (MR) vaccine, designated as MR-dCoV, administered to hamsters via intramuscular route, also protected against both SARS-CoV-2 challenges, and dCoV did not interfere with the MR vaccine-mediated immune response. The safety and efficacy of the dCoV and the MR-dCoV against both variants of SARS-CoV-2 opens the possibility of early immunization in children without an additional injection.
Household Disposal of Pharmaceuticals in Low-Income Settings: Practices, Health Hazards, and Research Needs
Authors: Gwenzi Willis , Simbanegavi Tinoziva T. , Rzymski Piotr
Published: January 2023

Pharmaceuticals are widely used in Africa due to the high burden of human and animal diseases. However, a review of the current practices and pollution risks arising from the disposal of pharmaceuticals in low-income settings in Africa is still lacking. Therefore, the present review examined the literature to address the following questions: (1) what are the key factors driving the accumulation of unused and expired pharmaceuticals?, (2) what are the current disposal practices for unused and expired pharmaceuticals, and wastewater (feces and urine) containing excreted pharmaceuticals?, (3) what are the potential environmental and human health hazards posed by current disposal practices?, and (4) what are the key research needs on the disposal of pharmaceuticals in low-income settings? Evidence shows that, in low-income settings, wastewater comprising predominantly of feces and urine containing excreted pharmaceuticals often end up in on-site sanitation systems such as pit latrines, septic tanks, and the environment in the case of open defecation. Unused and expired pharmaceuticals are disposed of in pit latrines, household solid waste, and/or burned. The pollution risks of current disposal practices are poorly understood, but pharmaceutical pollution of groundwater sources, including those used for drinking water supply, may occur via strong hydrological connectivity between pit latrines and groundwater systems. Potential high-risk pollution and human exposure hotspots are discussed. However, compared to other environmental compartments, the occurrence, dissemination, fate, and human health risks of pharmaceuticals in the pit latrine-groundwater continuum are still understudied. Future research directions are discussed to address these gaps using the Source-Pathway-Receptor-Impact-Mitigation (SPRIM) continuum as an organizing framework.
New approaches to treatment of neurological and psychopathological disorders in post-COVID syndrome
Authors:   City Clinical Hospital named after E. O. Mukhin of the Moscow Healthcare Department, Izvekov A. A. , Kapto O. V. ,   City Clinical Hospital named after E. O. Mukhin of the Moscow Healthcare Department, Khritinin D. F. ,   FSAEI HE I. M. Sechenov First Moscow State Medical University of the Ministry of Health of Russia, Tyan V. N. ,   FSBEI FPE Russian Medical Academy of Continuing Professional Education of the Ministry of Health of Russia, Katenko S. V. ,   FSAEI HE I. M. Sechenov First Moscow State Medical University of the Ministry of Health of Russia, Chaplyuk A. L. ,   FSBI Consultative and Diagnostic Polyclinic, 9th Medical and Diagnostic Center of the Ministry of Defense of Russia, Nagorskaya Yu. N. ,   FSBI Consultative and Diagnostic Polyclinic, 9th Medical and Diagnostic Center of the Ministry of Defense of Russia, Slastnikov V. Yu. ,   Branch No. 1 of the FSBI Main Military Clinical Hospital named after Academician N. N. Burdenko of the Ministry of Defense of Russia, Karakozov A. G. ,   FSBEI HE A. I. Yevdokimov Moscow State University of Medicine and Dentistry of the Ministry of Health of Russia, Molodova A. I. ,   FSBEI HE A. I. Yevdokimov Moscow State University of Medicine and Dentistry of the Ministry of Health of Russia, Slastnikova V. V. ,   Branch No. 1 of the FSBI Main Military Clinical Hospital named after Academician N. N. Burdenko of the Ministry of Defense of Russia, Khanykin V. S. ,   FSBEI HE A. I. Yevdokimov Moscow State University of Medicine and Dentistry of the Ministry of Health of Russia, Telyatnikov I. V. ,   FSBEI HE A. I. Yevdokimov Moscow State University of Medicine and Dentistry of the Ministry of Health of Russia
Published: January 2023

This article is devoted to the treatment of neurological and psychopathological disorders of post-COVID syndrome by reflexotherapy techniques.
Urgent issues of suicidal behavior in children and adolescents (general and regional aspects)
Authors:   State Budgetary Healthcare Institution of the Moscow Region «Central Clinical Psychiatric Hospital named after F. A. Usoltsev», Moscow, Russia, Berezantsev A. Yu. , Batueva N. G. ,   State Budgetary Healthcare Institution «Republican Neuropsychiatric Dispensary» of the Ministry of Health of the Republic of Buryatia, Ulan-Ude, Russia; Federal State budgetary Educational Institution of Higher Education «D. Banzarov Buryat State University», Ulan-Ude, Russia, Tudupova T. Ts. ,   Federal State budgetary Educational Institution of Higher Education «D. Banzarov Buryat State University», Ulan-Ude, Russia, Masyakin A. V. ,   State Budgetary Healthcare Institution of the Moscow Region «Central Clinical Psychiatric Hospital named after F. A. Usoltsev», Moscow, Russia
Published: January 2023

The article deals with the issue of suicidal behavior in children and adolescents in the context of the realities of modern society. There has been an increase in juvenile suicides in Russia in recent years, with an overall average decrease in suicide rates. An attempt is made to consider these facts in the context of social and biological aspects affecting the formation of behavioral disorders. The phenomenon of «fragmentary» or «clip» thinking, characteristic of the modern youth subculture, as well as the role of social and neurobiological factors in the formation of suicidal behavior, is discussed. The results of a retrospective analysis of complex posthumous forensic psychological and psychiatric examinations of completed suicides of children and adolescents in the Republic of Buryatia for the period 2012–2016 are presented. It has been found that male teenagers (15–17 years old) who lived in rural areas, were brought up in single-parent families, and studied at a secondary school predominate. In 21.8 % of cases, there was a history of suicide in close relatives and every fifth suicidal child had signs of suicidal behavior earlier (suicidal statements, attempts, interest in the topic of suicide). In most of the studied cases, family conflicts were the probable causes of suicidal behavior. It is concluded that in order to analyze the issue of suicide in children and adolescents, a comprehensive assessment of social, ethno-cultural, and biological factors is necessary.
Femmes et métiers de l’informatique
Authors: Rapkiewicz Clevi Elena
Published: January 2023

Clevi Elena Rapkiewicz : Women and computer professions.

This article presents a study of the place of women in the computer field. An analysis of the literature shows that, despite the important role played by some women in computer development, women have remained invisible. In addition, two field studies, one in Brazil and the other in France, make it possible to see concretely what is women’s participation today in this field.
Groundwater Potential in Unconfined Aquifers Using a Landform Approach in Gorontalo City
Authors: Maulana Karina Meiyanti , Santosa Langgeng Wahyu , Adji Tjahyo Nugroho
Published: January 2023

This research aimed to determine the potential of the unconfined aquifer in Gorontalo City based on the landform approach. The landforms in Gorontalo City consist of alluvial plains of lake deposits, floodplains of river deposits, alluvial fans of lake deposits, hills of structural fractures of reef limestones, hills of structural fractures of pinogu volcanic rocks, and hills of intrusive bone diorite. The method used consisted of a meteorological approach in the southern hills of Gorontalo City and a dynamic approach on the plains of Gorontalo City. The calculation of groundwater availability using a meteorological approach is 421.561,67 m3/year in the structural limestone reef fractures hill, 1.198.975,33 m3/year in the structural pinogu volcanic fractures hill, and 373.062,86 m3/year in the intrusive bone diorite hill, with a total of groundwater availability using the meteorological approach, is 1.993.599,87 m3/year. Groundwater availability using a dynamic approach was 2.621.535,19 m3/year or a discharge of 49,26 lt/sec (large) in the alluvial plain of lake sediment, discharge of 17,19 lt/sec (large) in the floodplain of river sediment, and discharge of 16,65 lt/sec (large) in the alluvial fan of lake sediment. The potential value of groundwater using the dynamic method is greater than the meteorological approach because of the amount of evapotranspiration, surface runoff, and crop coefficient.
Paleoenvironment of the Miocene Lemau Formation Based on the Palynology Analysis in Seluma, Bengkulu
Authors: Mentari Sakilla Gia , Winantris Winantris , Jurnaliah Lia
Published: January 2023

The Lemau Formation is a rock formation that forms the Bengkulu Basin in the Bengkulu Strip. The rocks in this formation contain hydrocarbon reserves in the form of coal. There has yet to be any research on the palynomorphs in the coal-bearing rocks in this formation. Palynomorph analysis was performed on nine samples from the P.T. Bara Indah Lestari, Seluma. The study aims to reconstruct the paleoenvironment and age of the coal-bearing rocks. The sampling technique uses stratigraphic measurements and hydrogen peroxide preparation. There were four changes in the depositional environment, namely the deposition of BIL 10-BIL 11 in the form of a freshwater peat swamp environment and BIL 12 in the form of a mangrove environment in the Early Miocene marked by the presence of Florschuetzia levipoli. The environment changed to a lagoon, indicated by the presence of foraminifera lining test and dinocyst in the deposition of BIL 13-BIL 15 samples in the Middle Miocene with a marked presence of Alnipollenites verus. The environment changed again during the deposition of BIL 16-BIL 18 to become a mangrove environment in the Middle Miocene. Overall, the paleoenvironment of the Lemau Formation is a freshwater peat swamp to a lagoon. Environmental changes are possible due to tectonic activity and climate change.
Contribution of Resistivity Properties in Estimating Hydraulic Conductivity in Ciremai Volcanic Deposits
Authors: Mutaqin Deden Zaenudin , Hendarmawan Hendarmawan , Haryanto Agus Didit , Mardiana Undang , Mohammad Febriwan
Published: January 2023

The hydraulic parameters of porous media, such as porosity (φ) and hydraulic conductivity (K), are the most important factors for planning and managing water exploitation from aquifers. This study aims to estimate the hydraulic conductivity parameters using the geoelectric method on volcanic deposits on the northern slope of Mount Ciremai. For this purpose, four data types were used to estimate K and φ, including lithological profiles, water table, groundwater quality, pumping test data, and vertical electrical sounding (VES). Based on Archie's law and Kozeny's equation, we get the alpha (α) values and cementation factor (m) from which the median values of α = 1.01 and m = 1.36 represent the studied aquifer.  The porosity (φ) of the aquifer varies from 0.097 to 0.187 with an average of 0.141 and is spatially related to the hydraulic conductivity (kgm), which varies from 4.97 × 10-6 to 6.75 × 10-5 m/s after the application of Kozeny's equation. The hydraulic conductivity (Kp) calculated from the pumping tests varies from 9.07 × 10-6 to 1.06 × 10-4 m/s and is strongly correlated (r = 0.87). Furthermore, a relation between resistivity and hydraulic conductivity was established for the studied aquifer to estimate these parameters in sites lacking data.
Transfer of technologies and systems of innovative activities
Authors:   National University  “Yuri Kondratyuk Poltava Polytechnic”, Mytrofanov Pavlo
Published: January 2023

Today, the systematic introduction of advanced technologies, taking into account the develop-ment of domestic industry, is an urgent issue. An important issue is the construction of relations between business and the scientific sector, taking into account the large number of scientific developments, which, unfortunately, are not implemented in production. Thus, technology transfer should ensure the establishment of a constructive dialogue between business and science. New knowledge obtained in research organizations as a result of scientific research work will be transferred to the commercial sector for public benefit.
Comparison of features of recognized top-5 innovative companies in the World
Authors:   Federal Institute of Parana, Sas Nataliia
Published: January 2023

Using the comparison method, the author analyzes the main activities of companies that allowed them to enter the top 5 innovative companies in the world and notes the industries in which innovations are most in demand and most promoted. Rating data for 2015–2022 were analyzed. Materials for comparison are summarized in a table.
Transformando el extremismo violento. Guía para el profesorado y la ciudadanía
Authors:   Universidad de Almeria, Rodríguez Martínez Pilar , Segura Sánchez Antonio ,   Universidad de Almería, López Berlanga María Carmen ,   Universidad de Almería, Martínez Martínez Ana María ,   Universidad de Almería
Published: January 2023

Transformando el extremismo violento es una guía para el profesorado y la ciudadanía, redactada para apoyar los esfuerzos de quienes trabajan en entornos educativos formales y no formales, dotándolos de un instrumento que les permite organizar sus ideas y desarrollar pedagogías transformativas para prevenir y actuar ante cualquier signo de extremismo violento, tanto en su vertiente cognitiva como conductual. El texto aborda el concepto de extremismo violento, definiéndolo y relacionándolo con otras violencias –cultural y estructural–. Se sitúa en el ámbito educativo, y se ofrecen ejemplos para detectar manifestaciones globales y locales de extremismo violento. Para transformarlo, se revisan algunos proyectos comunitarios transversales desarrollados en la Unión Europea, en España y en el ámbito local, destacando la necesidad de trabajar la inteligencia emocional con los grupos de pares, las familias y en el ciberespacio. También se ofrece una guía para que profesoras y profesores puedan manejar la situación en clase, deteniéndose en los objetivos, la preparación de la clase y la discusión. Se aportan ejemplos de temas para abordar y se propone cómo realizar la evaluación de la discusión y el seguimiento en el aula. En la última parte de la guía se relatan los valores implicados en la transformación del extremismo violento: la empatía, la solidaridad, el respeto a la diversidad, la convivencia y el compromiso. Al final se recogen algunos recursos electrónicos que permiten ampliar y profundizar en esta propuesta.
Analizando la tecnología y la gamificación educativas. Un acercamiento experimental
Authors:   Universidad de Zaragoza, Quintas Hijós Alejandro
Published: January 2023

Las tecnologías digitales y la gamificación son dos fenómenos que actualmente están en auge en el sistema educativo. En concreto, los videojuegos activos son una realidad en el ocio digital de los jóvenes y ya se están aplicado como innovación educativa. Sin embargo, desde una perspectiva analítica se tiene que examinar el planteamiento, mientras que, desde un acercamiento científico, se debe medir en las aulas reales la efectividad que se les presupone. Esta obra pretende ilustrar el proceso científico llevado a cabo en una investigación experimental realizada en distintos colegios de Educación Primaria, describiendo tanto el planteamiento filosófico y epistemológico inicial como los diseños concretos de investigación y de intervención educativa. El objetivo es compartir y promover un enfoque de investigación infrecuente en las ciencias de la educación, el experimental natural, aquel que pretende aproximarse tanto como sea posible al conocimiento de los efectos y las causas. Este enfoque científico permitirá mejorar la habitual justificación de la «experiencia personal» del maestro, en este caso en lo concerniente a la gamificación educativa y al videojuego como tecnología digital. Así, se han estudiado variables de interés para la didáctica como son el diseño programático, el método de enseñanza y el material educativo, además de características psicológicas, motrices y neurológicas del alumnado. La investigación presentada, explicada paso a paso, ha ayudado a esclarecer y poner a prueba las altas expectativas asignadas al potencial educativo de la gamificación y los videojuegos activos, sugiriendo una visión mucho más prudente y escéptica respecto a ellas.
ТУРИСТИЧНА ПРИВАБЛИВІСТЬ ЯВОРІВСЬКОГО РАЙОНУ ЛЬВІВСЬКОЇ ОБЛАСТІ УКРАЇНИ
Authors: Паньків Наталія , Ільчишин Олена
Published: January 2023

В статті розглянута туристична привабливість Яворівського району Львівської області України через призму природно-географічних переваг та культурно-історичних особливостей території. Зважаючи на прикордонне розташування району, значне природно-географічне різноманіття та наявність туристично привабливих культурно-історичних об’єктів, в межах району формується рекреаційно-туристичний поліфункціональний субрегіон, що спеціалізуватиметься на різновидовій туристичній діяльності. Так, основними напрямками розвитку туризму району є лікувально-оздоровча, екологічна та культурно-пізнавальна діяльність. В науковому  дослідженні проаналізовані територіальні особливості туристичної привабливості району через визначення пріоритетності окремих видів туристичної діяльності.
Psychological factors of the structure of social maladjustment of the individual in modern society
Authors: Orlenko Iryna
Published: January 2023

Today Ukrainian society is in a situation of socio-economic crisis, in a period when the integrity of political and geographical borders is violated, which causes an increase in the number of people who have fallen into difficult life circumstances, which finds its expression in the mechanism of «inclusion» of the individual in the social system. Changes occurring under the influence of crisis phenomena in our society reflect the state of internal dissonance of the individual. The intensive development of society and the problem of human adaptation to ever-changing living conditions and emergency situations necessitate a conceptual understanding of the phenomenon of social adaptation. Social adaptation processes require psychological understanding, which will make it possible to reveal deep relationships in the «person-society» system. The unstable state of society actualizes a number of social problems, the solution of which is aimed at both scientific thought and the system of social management. Therefore, it is the person who possesses the skills of social adaptation that is the main characteristic of a good society. ...
Mudanças de paradigmas realizados por mulheres negras na enfermagem
Authors: Santos Valéria Joaquim de Oliveira , Costa Jessica Gonçalves da , Brandão Fagner Alves Moreira , Moraes Filho Iel Marciano de
Published: January 2023

Falar da enfermagem é entender que o significado de cuidar é tão antigo quanto a existência humana e esse conceito de existir leva a uma reflexão sobre o “ser” negro em uma sociedade. Para tanto, no tange a sua luta por liberdade, na árdua trajetória por mudanças de paradigmas, o contexto histórico mundial traz a luta negra contra a escravidão, cujo o negro escravizado era tido como objeto, emergindo em um cenário de exclusão social, discriminação e intolerância
Importância do papel da enfermagem no atendimento à mulher vítima de violência e violência doméstica
Authors: Cordeiro Adriana dos Santos , Silva André Luis de Assis da , Fernandes Carla Thainá Santana , Nunes Denoelma Carvalho , Moraes Filho Iel Marciano de
Published: January 2023

Objetivo: descrever perante a literária as ações da enfermagem no cuidado a mulheres vítimas de violência doméstica, identificando falhas nas práticas e dimensões do cuidar. Método: Trata-se de um estudo de revisão narrativa da literatura, com abordagem qualitativa exploratória, realizada em abril de 2021, no qual foram abordados artigos científicos, pesquisados através dos Descritores em Ciências da Saúde: “Cuidados de enfermagem”, “Violência contra a mulher”, “Violência doméstica”, “Assistência à mulher”, “Lei Maria da Penha”, obtidos através das seguintes bases: Biblioteca Virtual Em Saúde; Literatura Latino-Americana e do Caribe em Ciências da Saúde, Scientific Eletronic Library On-line e documentos e normativas do Ministério da Saúde e do Conselho Federal de Enfermagem. Foram incluídos documentos publicados entre os anos de 2015 a 2021, que tinham ligação considerável com o tema proposto, e excluídos materiais que foram publicados antes de 2014 ou que não apresentavam ligação considerável com o tema. Resultados: Após a seleção dos documentos que atendiam aos critérios de inclusão e exclusão estabelecidos, foram selecionados 11, considerados pertinentes para o estudo. O enfermeiro se configura como o profissional que recebe as mulheres prioritariamente, tanto na atenção básica quanto nos prontos socorros, devendo, assim, proporcionar um diálogo amigável, gerando um elo de confiança entre profissional e vítima, promovendo intimidade e fornecendo segurança para que elas se sintam confortáveis para relatar o ocorrido e, por conseguinte, tomar as decisões prioritárias. Conclusão: A conduta do enfermeiro não seria possível sem a equipe multiprofissional e o auxílio da rede em saúde, frente aos cenários de violência contra a mulher.
Mortalidade pelo infarto agudo do miocárdio no Brasil: 1996- 2017
Authors: Nunes Adriano Alves , Benito Linconl Agudo Oliveira
Published: January 2023

Objetivo: Analisar a mortalidade pelo infarto agudo do miocárdio (IAM) no Brasil nos anos de 1996 a 2017. Método: Estudo epidemiológico, exploratório, descritivo e quantitativo. Os dados foram extraídos junto ao Serviço de Informação sobre Mortalidade (SIM) do Ministério da Saúde (MS). Foi realizada análise estatística descritiva. Resultados: Foi identificado o universo de 1.592.197 registros, com média e desvio padrão de (72.373±12.999,9). O ano de 2016 registrou a maior preponderância com 5,9% (n=94.148) e 1996 a menor com 3,5% (n=55.900). A maior preponderância se constituiu de 59,1% (n=940.552) pessoas do sexo masculino, 25,6% (n=407.340) possuíam entre 70 a 79 anos, 54,7% (n=871.319) possuíam cor/raça branca, 45,5% (n=725.234) eram casados, 20,7% (n=328.981) possuíam de 1 a 3 anos de escolarização, 55,6% (n=885.368) tiveram o registro de óbito no hospital. Conclusão: Foi identificado aumento na frequência de registros de óbito por IAM no recorte geográfico e histórico analisados.
Descritores: Mortalidade; Epidemiologia; Infarto agudo do miocárdio; Brasil
Identification of grapevine virus G and grapevine virus H in Sardinia, Italy
Authors: SCHIANCHI Nicola , FLORE Sandro , JAGUNIĆ Martin , SERRA Salvatorica , PROTA Vanda Assunta , VONČINA Darko
Published: January 2023

Grapevine virus G (GVG) and grapevine virus H (GVH) (genus Vitivirus) are recently discovered viruses. Analysis of 38 samples from Sardinian grapevine cultivars for the presence of GVG and GVH was carried out using RT-PCR. All samples were also tested for grapevine Pinot gris virus (GPGV) using RT-PCR, and for grapevine leafroll virus -1, -2 and -3, grapevine virus A (GVA) and B (GVB), arabis mosaic virus (ArMV), grapevine fanleaf virus (GFLV) and grapevine fleck virus (GFkV) using multiplex RT-PCR. GVG was confirmed in four vines, and GVH was detected in only one sample. In phylogenetic analyses of the coat protein (CP) region, the Sardinian GVG isolates clustered separately from isolates from Croatia and New Zealand. The Sardinian GVH isolate clustered with most sequences from other countries, but with greater affinity to isolates from California (USA) for the CP region, whereas it clustered with isolates from Croatia in the RNA-dependent RNA polymerase (RdRp) region. In addition to GVG and GVH, many samples were coinfected with GVA, viruses from the leafroll complex, and GPGV. This is the first record of GVG and GVH occurring in Italy.
Fusarium oxysporum f. sp. ginseng, a new forma specialis causing Fusarium root rot of Panax ginseng
Authors: WANG Jun , FENG Shi , LU Baohui , NA YANG Lina , WANG Xue , ZHANG Yanjing , GAO Jie
Published: January 2023

Panax ginseng is a valuable medicinal plant which is affected by many diseases during its long cultivation period. Ginseng root rot, caused by Fusarium oxysporum, has become severe in China. This soilborne pathogen comprises many formae speciales based on host specificity. Ten representative isolates from diseased ginseng rot root showed pathogenicity on ginseng. To identify the forma specialis of the F. oxysporum strains, host range tests of three representative isolates were carried out on nine plant species. All three isolates caused severe symptoms only on ginseng, but only slight or no visual symptoms on the other eight hosts, indicating that the strains were host-specific to ginseng. Phylogenetic trees were constructed based on sequences of the translation elongation factor 1-α (EF-1α) gene, two endopolygalacturonase genes (Pg1, Pg5) and two exopolygalacturonase genes (Pgx1 and Pgx4). The ten F. oxysporum isolates from ginseng clustered into a unique group clearly separated from other formae speciales already described. Based on the host range tests and phylogenetic analyses, the isolates of F. oxysporum derived from ginseng have been identified as a new forma specialis, designated Fusarium oxysporum f. sp. ginseng. This is the first report of forma specialis of F. oxysporum on ginseng.
Optimisation of sampling and testing for asymptomatic olive trees infected by Xylella fastidiosa in Apulia region, Italy
Authors: D'ONGHIA Anna Maria , SANTORO Franco , MINUTILLO Serena Anna , FRASHERI Dajana , GALLO Marilita , GUALANO Stefania , CAVALLO Giuseppe , VALENTINI Franco
Published: January 2023

Early detection of Xylella fastidiosa outbreaks in Apulian olive groves is crucial, especially in buffer zones and pathogen-free areas where olive trees are asymptomatic. Three studies were conducted. Two were on the spatial and temporal progression of X. fastidiosa infections in tree canopies of asymptomatic or mildly symptomatic olive trees of tolerant (‘Leccino’) and susceptible (‘Cellina di Nardò’ and ‘Ogliarola salentina’) cultivars. Despite different trends in pathogen infection rates and concentrations between ‘Leccino’ and susceptible olive cultivars over the study period, results showed that sampling was most effective in the mid-upper part of tree canopies throughout the year, excluding the warmest and coldest periods. Stem xylem tissues were the most appropriate for detecting the pathogen compared to lower parts of mature leaves with petioles, using serological and molecular assays. Based on these results, a third study was conducted to compare molecular and serological tests (qPCR, real-time LAMP, DAS-ELISA, DTBIA) for detection of X. fastidiosa in the mid-upper part of asymptomatic branches of infected ‘Leccino’ trees that were sampled in an appropriate collection time, using stem xylem tissue as the most appropriate matrix for testing. The molecular methods showed the greatest sensitivity, with no undetermined results, while among the serological assays, DTBIA was more sensitive than DAS-ELISA. An improved protocol for monitoring asymptomatic olive trees is recommended.
Nanoplate digital PCR assays for detection and quantification of Xylella fastidiosa
Authors: PASSERA Alessandro , GROSSO Valentina , MIOTTI Niccolò , ROSSATO Marzia , GAFFURI Francesca , CASATI Paola , DELLEDONNE Massimo , BIANCO Piero
Published: January 2023

Xylella fastidiosa is a fastidious Gram-negative bacterium that is associated with several important plant diseases, and is regulated as a quarantine pest in many countries where strategies are implemented to prevent its introduction and spread. To enact efficient quarantine measures, effective and early detection of the pathogen are essential, especially because global trade of goods increases the risks of introduction of alien pathogens. this study aimed to adapt two qPCR-based diagnostic methods (SYBR Green and Probe based qPCR), already in use to detect X. fastidiosa, for use with a nanoplate based digital PCR assay. Detection of the pathogen using the two digital PCR assays (EvaGreen- and Probe-based) was similar to standard qPCR, giving 100% sensitivity, specificity, and accuracy, while providing accurate absolute quantification of the pathogen when using experimental samples that had low concentrations of host DNA. Using undiluted plant DNA added with low concentrations of X. fastidiosa, only the TaqMan method maintained satisfactory performance and quantification, and is therefore preferred. These results are a first step demonstrating the usefulness of nanoplate-based digital PCR for detection of plant pathogens, which allows greater throughput than qPCR, reducing the time and cost of diagnostic assays.
Response of carrot seed germination to heat treatment, the emergency measure to reduce the risk of ‘Candidatus Liberibacter solanacearum’ seed transmission
Authors: TIZZANI Lorenza , GENTILI Andrea , TAGLIENTI Anna , BERTIN Sabrina , ILARDI Vincenza
Published: January 2023

In Europe and the Mediterranean region, ‘Candidatus Liberibacter solanacearum’ (Lso) is associated with emerging diseases of Apiaceae crops, mainly carrot. Emergency measures for import of carrot seed were set, requiring seed to be heat-treated at 50°C or tested as Lso-negative by PCR. The germination response to heat treatment was assessed for 24 carrot cultivar and hybrid seed lots. Ten parsley, five fennel, and two celery seed lots were also analysed. Of these 41 seed lots, 21 were Lso-infected. Water heat treatment significantly decreased germinability compared to dry heat treatment, indicating that dry heat treatment is a cheaper and less detrimental procedure. However, the dry heat treatment significantly decreased seed germination compared to untreated controls in four of 24 seed lots of carrot, four of ten parsley seed lots, three of five fennel seed lots, and one of two celery seed lots. For parsley, the heat treatment reduced germinability to a lesser extent in Lso-infected than Lso-free seed lots. These data show that heat treatment can affect the germination of Apiaceae seeds to varying degrees, depending on species or variety, the type of heat treatment, and the sanitary status of the seeds.
Plant Health research collaboration in the Mediterranean region: case studies on citrus tristeza virus, tomato brown rugose fruit virus and Xylella fastidiosa
Authors: GIOVANI Baldissera , BOUTIGNY Anne-Laure , DJELOUAH Khaled , FOX Adrian , D’ONGHIA Anna Maria
Published: January 2023

Strengthening Plant Health research is a major challenge for Mediterranean countries. The diversity and fragmentation of the research landscape in this region have weakened the impacts of national efforts. Mediterranean countries can benefit from coordination of research activities to increase efficiency and impacts. The initiative ‘Plant health research priorities for the Mediterranean region’, led by the International Centre for Advanced Mediterranean Agronomic Studies (CIHEAM Bari)1 and the Euphresco network for phytosanitary research coordination and funding2 can promote convergence of national programmes and optimize the use of the scarce funding available to plant health, thus strengthening international cooperation and increasing the excellence and relevance of research.