bluuebunny/crossref_metadata_2025 huggingface dataset:
H2O Collisional Broadening Coefficients at 1.37 µm and Their Temperature Dependence: A Metrology Approach
Authors: Nwaboh Javis A. , Werhahn Olav , Ebert Volker
Published: June 2021

We report self- and air collisional broadening coefficients for the H2O line at 7299.43 cm−1 and corresponding temperature coefficients for a temperature range spanning 293–573 K. New laser spectroscopic setups specifically designed for this purpose have been developed and are described. The line parameters reported here are in good agreement with those values reported in the HITRAN 2020 database, but the uncertainties have been reduced by factors of about 4, 1.3 and 4.4 for the self-broadening coefficient, air broadening coefficient and the temperature exponent of air broadening, respectively. Further, we combined our measurement approach with metrological data quality objectives, addressing the traceability of the results to the international system of units (SI) and evaluated the uncertainties following the guide to the expression of uncertainty in measurement (GUM).
Soft Robotic Hands and Tactile Sensors for Underwater Robotics
Authors: Subad Rafsan Al Shafatul Islam , Cross Liam B. , Park Kihan
Published: June 2021

Research in the field of underwater (UW) robotic applications is rapidly developing. The emergence of coupling the newest technologies on submersibles, different types of telecommunication devices, sensors, and soft robots is transforming the rigid approach to robotic design by providing solutions that bridge the gap between accuracy and adaptability in an environment where there is so much fluctuation in object targeting and environmental conditions. In this paper, we represent a review of the history, development, recent research endeavors, and projected outlook for the area of soft robotics technology pertaining to its use with tactile sensing in the UW environment.
Improvement in Modeling of OH and HO2 Radical Concentrations during Toluene and Xylene Oxidation with RACM2 Using MCM/GECKO-A
Authors: Lannuque Victor , D’Anna Barbara , Couvidat Florian , Valorso Richard , Sartelet Karine
Published: June 2021

Due to their major role in atmospheric chemistry and secondary pollutant formation such as ozone or secondary organic aerosols, an accurate representation of OH and HO2 (HOX) radicals in air quality models is essential. Air quality models use simplified mechanisms to represent atmospheric chemistry and interactions between HOX and organic compounds. In this work, HOX concentrations during the oxidation of toluene and xylene within the Regional Atmospheric Chemistry Mechanism (RACM2) are improved using a deterministic–near-explicit mechanism based on the Master Chemical Mechanism (MCM) and the generator of explicit chemistry and kinetics of organics in the atmosphere (GECKO-A). Flow tube toluene oxidation experiments are first simulated with RACM2 and MCM/GECKO-A. RACM2, which is a simplified mechanism, is then modified to better reproduce the HOX concentration evolution simulated by MCM/GECKO-A. In total, 12 reactions of the oxidation mechanism of toluene and xylene are updated, making OH simulated by RACM2 up to 70% more comparable to the comprehensive MCM/GECKO-A model for chamber oxidation simulations.
Gender Specific Differences in Disease Susceptibility: The Role of Epigenetics
Authors: Migliore Lucia , Nicolì Vanessa , Stoccoro Andrea
Published: June 2021

Many complex traits or diseases, such as infectious and autoimmune diseases, cancer, xenobiotics exposure, neurodevelopmental and neurodegenerative diseases, as well as the outcome of vaccination, show a differential susceptibility between males and females. In general, the female immune system responds more efficiently to pathogens. However, this can lead to over-reactive immune responses, which may explain the higher presence of autoimmune diseases in women, but also potentially the more adverse effects of vaccination in females compared with in males. Many clinical and epidemiological studies reported, for the SARS-CoV-2 infection, a gender-biased differential response; however, the majority of reports dealt with a comparable morbidity, with males, however, showing higher COVID-19 adverse outcomes. Although gender differences in immune responses have been studied predominantly within the context of sex hormone effects, some other mechanisms have been invoked: cellular mosaicism, skewed X chromosome inactivation, genes escaping X chromosome inactivation, and miRNAs encoded on the X chromosome. The hormonal hypothesis as well as other mechanisms will be examined and discussed in the light of the most recent epigenetic findings in the field, as the concept that epigenetics is the unifying mechanism in explaining gender-specific differences is increasingly emerging.
Prognostic Relevance of CD4+, CD8+ and FOXP3+ TILs in Oral Squamous Cell Carcinoma and Correlations with PD-L1 and Cancer Stem Cell Markers
Authors: Lequerica-Fernández Paloma , Suárez-Canto Julián , Rodriguez-Santamarta Tania , Rodrigo Juan Pablo , Suárez-Sánchez Faustino Julián , Blanco-Lorenzo Verónica , Domínguez-Iglesias Francisco , García-Pedrero Juana María , de Vicente Juan Carlos
Published: June 2021

This study investigates the relevance of tumor-infiltrating lymphocytes (TILs) in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis of stromal/tumoral CD4+, CD8+ and FOXP3+ TILs is performed in 125 OSCC patients. Potential relationships with the expression of tumoral PD-L1 and cancer stem cell (CSC) markers (NANOG, SOX2, OCT4, Nestin and Podoplanin (PDPN)) are assessed. CD4+ and CD8+ TILs are significantly associated with smoking and alcohol habits. CD4+ and CD8+ TILs show an inverse relationship with NANOG and SOX2 expression, and FOXP3+ TILs is significantly correlated with Nestin and PDPN expression. High infiltration of CD4+ and CD8+ TILs and a high tumoral CD8+/FOXP3+ ratio are significantly associated with tumors harboring positive PD-L1 expression. Infiltration of stromal/tumoral FOXP3+ TILs and a low stromal CD8+/FOXP3+ ratio are significantly associated with better disease-specific survival. Multivariate analysis reveals that the stromal CD8+/FOXP3+ TILs ratio is a significant independent prognostic factor. Regarding OSCC patient survival, the CD8+/FOXP3+ TILs ratio is an independent prognostic factor. TILs may act as biomarkers and potential therapeutic targets for OSCC.
Emerging Patterns in Cultural Ecosystem Services as Incentives and Obstacles for Raptor Conservation
Authors: Horgan Finbarr G. , Mundaca Enrique A. , Crisol-Martínez Eduardo
Published: June 2021

The Millennium Ecosystem Assessment proposed four categories of ecosystem services as regulating, provisioning, supporting and cultural. Of these, cultural services have been the most difficult to quantify despite playing a key role in developing society’s supporting services to ecosystems. By reviewing a series of case studies related to the cultural services derived from raptors, we examine relations between tangible ecosystem services and ‘knowledge’ and ‘beliefs’ as part of supporting services from human societies to ecosystems. We identified types of raptor regulating and provisioning services and patterns in service--knowledge-beliefs that defined positive or negative outcomes for raptor conservation. We also demonstrate how possible interactions between physical, experiential, physical-symbolic and representative-symbolic cultural services and between different stakeholders can create incentives or obstacles for conservation. Predictable patterns in service-knowledge-beliefs provide a framework upon which socio-cultural and ethnobiological aspects of raptor conservation may be combined with ecological research to support conservation initiatives. Based on these patterns we present examples of how cultural services might be employed to better promote raptor conservation while respecting the beliefs and traditions of stakeholders.
Update on Neuropathies in Inborn Errors of Metabolism
Authors: Pająk Renata , Mendela Ewelina , Będkowska Natalia , Paprocka Justyna
Published: June 2021

Neuropathies are relatively common in inborn errors of metabolism (IEMs); however, due to the early onset and severe, progressive course of many IEMs, they have not been very well researched yet. This article aims to review and compare neuropathies in inborn errors of metabolism, mostly with childhood and juvenile onset. Some of these diseases are treatable if diagnosed early and in many cases, the therapy can not only slow down disease progression, but can also reverse the changes already made by the condition.
Bispecific T Cell Engagers for the Treatment of Multiple Myeloma: Achievements and Challenges
Authors: Alhallak Kinan , Sun Jennifer , Jeske Amanda , Park Chaelee , Yavner Jessica , Bash Hannah , Lubben Berit , Adebayo Ola , Khaskiah Ayah , Azab Abdel Kareem
Published: June 2021

MM is the second most common hematological malignancy and represents approximately 20% of deaths from hematopoietic cancers. The advent of novel agents has changed the therapeutic landscape of MM treatment; however, MM remains incurable. T cell-based immunotherapy such as BTCEs is a promising modality for the treatment of MM. This review article discusses the advancements and future directions of BTCE treatments for MM.
[6]-Gingerol-Derived Semi-Synthetic Compound SSi6 Inhibits Tumor Growth and Metastatic Dissemination in Triple-Negative Breast Cancer Xenograft Models
Authors: Luna-Dulcey Liany , Almada da Silva James , Jimenez-Renard Veronica , Caleiras Eduardo , Mouron Silvana , Quintela-Fandino Miguel , Cominetti Marcia R.
Published: June 2021

Breast cancer metastasis is the most common cause of cancer death in women worldwide. Triple-negative breast cancers (TNBC) form a heterogeneous group of tumors that have higher relapse rates and poorer survival compared to other breast cancer subtypes. Thus, this work reports the antitumor and antimetastatic activities of a [6]-gingerol-derived semi-synthetic compound named SSi6 on MDA-MB-231 TNBC cells using xenograft models. SSi6 did not cause toxic effects in vivo as demonstrated by body weight and hematological and histological evaluations. From the orthotopic xenograft model, we demonstrated that SSi6 slows and inhibits the growth of the primary tumor, as well as prevents metastatic spontaneous progression from lymph nodes to the lungs. Moreover, a second xenograft model with resection of the primary tumor showed that SSi6 also blocks the progression of metastases from the lymph nodes to other visceral organs. Taken together, our results demonstrate that SSi6 is a promising compound to be investigated in other preclinical and clinical models to be applied as a complementary therapy for TNBC.
Associations of General and Abdominal Obesity with the Risk of Glioma Development
Authors: Ahn Stephen , Han Kyungdo , Lee Jung-Eun , Jeun Sin-Soo , Park Yong-Moon , Yang Seung Ho
Published: June 2021

The association between obesity and the risk of glioma remains unclear. We sought to evaluate the potential association between general and abdominal obesity and the risk of glioma based on a nationwide population-based cohort study of Koreans. Using data from the Korean National Health Insurance System cohort, 6,833,744 people older than 20 years who underwent regular national health examination in both 2009 and 2011 were followed until the end of 2017. We documented 4771 glioma cases based on an ICD-10 code of C71 during the median follow-up period of 7.30 years. Individuals with a body mass index (BMI) ≥ 25.0 kg/m2 were at significantly higher risk of developing glioma than those with a BMI < 25.0 kg/m2 (HR 1.08 CI 1.02–1.15). Individuals with a waist circumference (WC) ≥ 90 cm (males)/85 cm (females) also had a significantly higher risk of glioma than those with a WC < 90 cm (males)/85 cm (females) (HR 1.16 CI 1.09–1.24). In the group with a BMI ≥ 25.0 kg/m2, individuals with abdominal obesity were at significantly higher risk of developing glioma (HR 1.18 CI 1.09–1.27) than those without abdominal obesity. The role of abdominal obesity in this association was stronger in women than in men. To the best of our knowledge, this is the first demonstration that obese people may be at higher risk of glioma, especially centrally obese people from an Asian population with a BMI ≥ 25.0 kg/m2. Loss of visceral fat in people with abdominal obesity may reduce their risk of developing glioma.
Usefulness of Imaging Response Assessment after Irreversible Electroporation of Localized Pancreatic Cancer—Results from a Prospective Cohort
Authors: Flak Rasmus V. , Fisker Rune V. , Bruun Niels H. , Stender Mogens T. , Thorlacius-Ussing Ole , Petersen Lars J.
Published: June 2021

(1) Background: Irreversible electroporation (IRE) is a nonthermal ablation technique that is being studied in nonmetastatic pancreatic cancer (PC). Most published studies use imaging outcomes as an efficacy endpoint, but imaging interpretation can be difficult and has yet to be correlated with survival. The aim of this study was to examine the correlation of imaging endpoints with survival in a cohort of IRE-treated PC patients. (2) Methods: Several imaging endpoints were examined before and after IRE on 18F-fluorodeoxyglucose positron emission tomography (PET) with computed tomography. Separate analyses were performed at the patient and lesion levels. Mortality rate (MR) ratios for imaging endpoints after IRE were estimated. (3) Results: Forty-one patients were included. Patient-level analysis revealed that progressive disease (PD), as defined by RECIST 1.1, is correlated with a higher MR at all time intervals, but PD, as defined by EORTC PET response criteria, is only correlated with the MR in the longest interval. No correlation was found between PD, as defined by RECIST, and the MR in the lesion-level analysis. (4) Conclusions: Patient-level PD, as defined by RECIST, was correlated with poorer survival after IRE ablation, whereas no correlations were observed in the lesion-level analyses. Several promising lesion-level outcomes were identified.
Loss of Paid Employment up to 4 Years after Colorectal Cancer Diagnosis—A Nationwide Register-Based Study with a Population-Based Reference Group
Authors: de Wind Astrid , Tamminga Sietske J. , Bony Claudia A. G. , Diether Maren , Ludwig Martijn , Velthuis Miranda J. , Duijts Saskia F. A. , de Boer Angela G. E. M.
Published: June 2021

Cancer survivors consider work as a key aspect of cancer survivorship while previous research indicated that cancer survivors have a higher risk of unemployment. The objectives were to assess: (1) whether colorectal cancer survivors less often have paid employment at diagnosis compared to a population-based reference group, (2) whether colorectal cancer survivors with paid work have a higher risk of loss of employment up to 4 years after diagnosis compared to a population-based reference group and (3) which colorectal cancer survivors are at highest risk of loss of paid employment. In a nationwide register-based study, persons diagnosed with colorectal cancer (N = 12,007) as registered in the Netherlands Cancer Registry, were compared on loss of paid employment with a sex and age-matched population-based reference group (N = 48,028) from Statistics Netherlands. Cox regression analyses were conducted. Colorectal cancer survivors had a higher risk of loss of paid employment (HR 1.56 [1.42, 1.71]). Within the group of colorectal cancer survivors, risk of loss of paid employment was lower for older survivors (>60 vs. 45–55) (HR 0.64 [0.51, 0.81]) and higher for those with a more advanced cancer stage (IV vs. I) (HR 1.89 [1.33, 2.70]) and those receiving radiotherapy (HR 1.37 [1.15, 1.63]). Colorectal cancer survivors at high risk of loss of paid employment may benefit from work support interventions as part of cancer survivorship.
Synthesis and Characterization of Cobalt and Nitrogen Co-Doped Peat-Derived Carbon Catalysts for Oxygen Reduction in Acidic Media
Authors: Jäger Rutha , Teppor Patrick , Paalo Maarja , Härmas Meelis , Adamson Anu , Volobujeva Olga , Härk Eneli , Kochovski Zdravko , Romann Tavo , Härmas Riinu , Aruväli Jaan , Kikas Arvo , Lust Enn
Published: June 2021

In this study, several peat-derived carbons (PDC) were synthesized using various carbonization protocols. It was found that depending on the carbonization method, carbons with very different surface morphologies, elemental compositions, porosities, and oxygen reduction reaction (ORR) activities were obtained. Five carbons were used as carbon supports to synthesize Co-N/PDC catalysts, and five different ORR catalysts were acquired. The surface analysis revealed that a higher nitrogen content, number of surface oxide defects, and higher specific surface area lead to higher ORR activity of the Co-N/PDC catalysts in acidic solution. The catalyst Co-N/C-2(ZnCl2), which was synthesized from ZnCl2-activated and pyrolyzed peat, showed the highest ORR activity in both rotating disk electrode and polymer electrolyte membrane fuel cell tests. A maximum power density value of 210 mW cm−2 has been obtained. The results of this study indicate that PDCs are promising candidates for the synthesis of active non-platinum group metal type catalysts.
Theoretical Insights into the Hydrogen Evolution Reaction on the Ni3N Electrocatalyst
Authors: Cross Russell W. , Rondiya Sachin R. , Dzade Nelson Y.
Published: June 2021

Ni-based catalysts are attractive alternatives to noble metal electrocatalysts for the hydrogen evolution reaction (HER). Herein, we present a dispersion-corrected density functional theory (DFT-D3) insight into HER activity on the (111), (110), (001), and (100) surfaces of metallic nickel nitride (Ni3N). A combination of water and hydrogen adsorption was used to model the electrode interactions within the water splitting cell. Surface energies were used to characterise the stabilities of the Ni3N surfaces, along with adsorption energies to determine preferable sites for adsorbate interactions. The surface stability order was found to be (111) < (100) < (001) < (110), with calculated surface energies of 2.10, 2.27, 2.37, and 2.38 Jm−2, respectively. Water adsorption was found to be exothermic at all surfaces, and most favourable on the (111) surface, with Eads = −0.79 eV, followed closely by the (100), (110), and (001) surfaces at −0.66, −0.65, and −0.56 eV, respectively. The water splitting reaction was investigated at each surface to determine the rate determining Volmer step and the activation energies (Ea) for alkaline HER, which has thus far not been studied in detail for Ni3N. The Ea values for water splitting on the Ni3N surfaces were predicted in the order (001) < (111) < (110) < (100), which were 0.17, 0.73, 1.11, and 1.60 eV, respectively, overall showing the (001) surface to be most active for the Volmer step of water dissociation. Active hydrogen adsorption sites are also presented for acidic HER, evaluated through the ΔGH descriptor. The (110) surface was shown to have an extremely active Ni–N bridging site with ΔGH = −0.05 eV.
Inhibition of Human Malignant Pleural Mesothelioma Growth by Mesenchymal Stromal Cells
Authors: Coccè Valentina , La Monica Silvia , Bonelli Mara , Alessandri Giulio , Alfieri Roberta , Lagrasta Costanza Annamaria , Madeddu Denise , Frati Caterina , Flammini Lisa , Lisini Daniela , Marcianti Angela , Parati Eugenio , Paino Francesca , Giannì Aldo , Farronato Giampietro , Falco Angela , Spaggiari Lorenzo , Petrella Francesco , Pessina Augusto
Published: June 2021

Background: Malignant Pleural Mesothelioma (MPM) is an aggressive tumor that has a significant incidence related to asbestos exposure with no effective therapy and poor prognosis. The role of mesenchymal stromal cells (MSCs) in cancer is controversial due to their opposite effects on tumor growth and in particular, only a few data are reported on MSCs and MPM. Methods: We investigated the in vitro efficacy of adipose tissue-derived MSCs, their lysates and secretome against different MPM cell lines. After large-scale production of MSCs in a bioreactor, their efficacy was also evaluated on a human MPM xenograft in mice. Results: MSCs, their lysate and secretome inhibited MPM cell proliferation in vitro with S or G0/G1 arrest of the cell cycle, respectively. MSC lysate induced cell death by apoptosis. The efficacy of MSC was confirmed in vivo by a significant inhibition of tumor growth, similar to that produced by systemic administration of paclitaxel. Interestingly, no tumor progression was observed after the last MSC treatment, while tumors started to grow again after stopping chemotherapeutic treatment. Conclusions: These data demonstrated for the first time that MSCs, both through paracrine and cell-to-cell interaction mechanisms, induced a significant inhibition of human mesothelioma growth. Since the prognosis for MPM patients is poor and the options of care are limited to chemotherapy, MSCs could provide a potential new therapeutic approach for this malignancy.
Carboxylated Poly-l-Lysine as a Macromolecular Cryoprotective Agent Enables the Development of Defined and Xeno-Free Human Sperm Cryopreservation Reagents
Authors: Takeuchi Hiroki , Nishioka Mikiko , Maezawa Tadashi , Kitano Yuko , Terada-Yoshikawa Kento , Tachibana Ryota , Kato Manabu , Hyon Suong-hyu , Gen Yuki , Tanaka Kayo , Toriyabe Kuniaki , Nii Masafumi , Kondo Eiji , Ikeda Tomoaki
Published: June 2021

In human sperm cryopreservation, test yolk buffer and human serum albumin have been used as permeating macromolecular-weight cryoprotectants. In clinical reproductive medicine, human serum albumin is frequently used because of low risks of zoonoses and allergic reactions. However, the risk of allogeneic infectious diseases exists, and the supply may be unstable because human serum albumin is derived from human blood. Therefore, the development of xeno-free human sperm cryopreservative reagents that could overcome the aforementioned problems is warranted. We succeeded in developing a new xeno-free and defined sperm cryopreservation reagent containing glycerol, carboxylated poly-l-lysine, and raffinose. The cryopreservation reagent was not significantly different in terms of sperm motility, viability, and DNA fragmentation and was comparable in performance to a commercial cryopreservation reagent containing human serum albumin. Moreover, the addition of saccharides was essential for its long-term storage. These results may help elucidate the unknown function of macromolecular-weight permeating cryoprotective agents.
In Vitro Examinations of Cell Death Induction and the Immune Phenotype of Cancer Cells Following Radiative-Based Hyperthermia with 915 MHz in Combination with Radiotherapy
Authors: Hader Michael , Streit Simon , Rosin Andreas , Gerdes Thorsten , Wadepohl Martin , Bekeschus Sander , Fietkau Rainer , Frey Benjamin , Schlücker Eberhard , Gekle Stephan , Gaipl Udo S.
Published: June 2021

Multimodal tumor treatment settings consisting of radiotherapy and immunomodulating agents such as immune checkpoint inhibitors are more and more commonly applied in clinics. In this context, the immune phenotype of tumor cells has a major influence on the anti-tumor immune response as well as the composition of the tumor microenvironment. A promising approach to further boost anti-tumor immune responses is to add hyperthermia (HT), i.e., heating the tumor tissue between 39 °C to 45 °C for 60 min. One key technique is the use of radiative hyperthermia systems. However, knowledge is limited as to how the frequency of the used radiative systems affects the immune phenotype of the treated tumor cells. By using our self-designed in vitro hyperthermia system, we compared cell death induction and expression of immune checkpoint molecules (ICM) on the tumor cell surface of murine B16 melanoma and human MDA-MB-231 and MCF-7 breast cancer cells following HT treatment with clinically relevant microwaves at 915 MHz or 2.45 GHz alone, radiotherapy (RT; 2 × 5 Gy or 5 × 2 Gy) alone or in combination (RHT). At 44 °C, HT alone was the dominant cell death inductor with inactivation rates of around 70% for B16, 45% for MDA-MB-231 and 35% for MCF-7 at 915 MHz and 80%, 60% and 50% at 2.45 GHz, respectively. Additional RT resulted in 5–15% higher levels of dead cells. The expression of ICM on tumor cells showed time-, treatment-, cell line- and frequency-dependent effects and was highest for RHT. Computer simulations of an exemplary spherical cell revealed frequency-dependent local energy absorption. The frequency of hyperthermia systems is a newly identified parameter that could also affect the immune phenotype of tumor cells and consequently the immunogenicity of tumors.
Improved Catalytic Activity of the High-Temperature Water Gas Shift Reaction on Metal-Exsolved La0.9Ni0.05Fe0.95O3 by Controlling Reduction Time
Authors: Huang Rui , Han Jeong Woo
Published: June 2021

The catalyst exsolved from nickel-doped perovskite oxide, La0.9Ni0.05Fe0.95O3, has been proven to be effective for gas-phase reactions. To obtain the optimum amount of exsolved nanoparticles from the parent perovskite oxide, control of the reduction treatment condition is vital. Here, the effect of reduction time on the exsolved nanoparticle distribution, and thus the catalytic activity of the high-temperature water gas shift reaction (WGSR), was investigated. Upon conducting a wide range of characterizations, we assumed that the exsolution process might be a two-step process. Firstly, the surface oxygen is extracted. Secondly, due to the unstable perovskite structure, the Ni ions in the bulk La0.9Ni0.05Fe0.95O3 continuously diffuse toward the surface and, as the reduction progresses, more nuclei are generated to form a greater number of nanoparticles. This assumption is proven by the fact that, with an increase in the exsolution treatment time, the population of exsolution nanoparticles increases. Moreover, as the reduction time increases, the high-temperature WGSR activity also increases. The temperature-programmed measurements suggest that the exsolved nanoparticles are the active reaction sites. We believe that this study is helpful for understanding exsolution behavior during reduction treatment and, thus, developing a perovskite exsolution catalyst for the WGSR.
Reconstruction of the Hip in Multiple Hereditary Exostoses
Authors: Lee Dong Hoon , Paley Dror
Published: June 2021

The hip joint involvement in multiple hereditary exostoses (MHE) occurs in 30–90%, causing pain and limitation of motion by femoroacetabular impingement, coxa valga, acetabular dysplasia, hip joint subluxation, and osteoarthritis. The purpose of this study was to investigate the clinical and radiographic outcomes of ten hips in seven patients treated by surgical dislocation and corrective osteotomies between 2004 and 2009. Surgical dislocation and excision of the osteochondromas and varus intertrochanteric osteotomies were performed in all cases when the neck–shaft angle was > 150°. Common sites of osteochondromas were medial, posterior, and anterior neck of the femur. Neck–shaft angle of the femur was improved from a mean of 157° to 139°, postoperatively. On an average, the center-edge angle improved from 20° to 30° postoperatively. We believe that Ganz’s safe surgical dislocation technique is the preferred treatment of MHE. This safeguards the circulation of the femoral head and the osteochondromas can be resected under direct vision. It can be combined with additional corrective osteotomies because the hip affected by MHE is frequently associated with dysplastic changes which can result in premature osteoarthritis.
Differentially Expressed Extracellular Vesicle-Contained microRNAs before and after Transurethral Resection of Bladder Tumors
Authors: Strømme Olaf , Heck Kathleen A. , Brede Gaute , Lindholm Håvard T. , Otterlei Marit , Arum Carl-Jørgen
Published: June 2021

Bladder cancer (BC) is currently diagnosed and monitored by cystoscopy, a costly and invasive procedure. Potential biomarkers in urine, blood, and, more recently, extracellular vesicles (EVs), have been explored as non-invasive alternatives for diagnosis and surveillance of BC. EVs are nanovesicles secreted by most cell types containing diverse molecular cargo, including different types of small RNAs, such as microRNA (miRNA). In this study, we performed next-generation sequencing of EV-contained miRNA isolated from urine and serum of 41 patients with non-muscle invasive BC (27 stage Ta, 14 stage T1) and 15 non-cancer patients (NCP) with benign cystoscopy findings. MiRNA sequencing was also performed on serum supernatant samples for T1 patients. To identify potential BC-specific biomarkers, expression levels of miRNA in presurgery samples were compared to those at postsurgery check-ups, and to NCPs. Results showed that two miRNAs, urinary EV-contained miR-451a and miR-486-5p, were significantly upregulated in presurgery samples from T1 patients compared to postsurgery check-up samples. This was confirmed in a replica EV/RNA isolation and sequencing run of 10 T1 patients from the primary run; however, analyses revealed no differential expression of miRNAs in serum EVs, serum supernatant, or when comparing BC patients to NCPs. This is the first study to investigate EV-containing miRNA sequencing in pre- and postsurgery BC patient samples and our findings suggest that urinary EV-contained miR-451a and miR-486-5p may be potential biomarkers for recurrence-free survival of BC patients with stage T1 disease.
Poly(ADP-Ribose) Polymerase Inhibitor PJ34 Reduces Brain Damage after Stroke in the Neonatal Mouse Brain
Authors: Bonnin Philippe , Vitalis Tania , Schwendimann Leslie , Boutigny Alexandre , Mohamedi Nassim , Besson Valérie C , Charriaut-Marlangue Christiane
Published: June 2021

The poly(ADP-ribose) polymerase inhibitor PJ34 has recently been reported to increase cerebral blood flow, via the endothelial NO synthase, in the naive mouse brain throughout life. We addressed here the benefits of PJ34 after neonatal ischemia on hemodynamics and components of the neurovascular unit including the blood-brain barrier (BBB), microglia, and astrocytes. Nine-day-old mice were subjected to permanent MCA occlusion (pMCAo), and treated with either PBS or PJ34 (10 mg/kg). Mean blood-flow velocities (mBFV) were measured in both internal carotid arteries (ICA) and basilar trunk (BT) using Doppler-ultrasonography. BBB opening was assessed through somatostatin-receptor type-2 internalization and immunohistochemistry at 24 and 48 h. Lesion areas were measured 8 days after ischemia. In PBS-treated mice, pMCAo involved a drop in mBFV in the left ICA (p < 0.001 vs. basal), whereas mBFV remained stable in both right ICA and BT. PJ34 prevented this drop in the left ICA (NS vs. basal) and increased mBFV in the right ICA (p = 0.0038 vs. basal). No modification was observed in the BT. In contrast to PBS, BBB disruption extent and astrocyte demise were reduced in PJ34 mice only in the rostral brain at 48 h and 8 days post-pMCAo, respectively. Accordingly, 8 days after pMCAo, affected areas were reduced in the rostral brain (Bregma +0.86 and +0.14 mm), whereas total tissue loss was not reduced after PJ34 (4.0 ± 3.1%) vs. PBS (5.8 ± 3.4%). These results show that PJ34 reduced BBB permeability, astrocyte demise, and tissue loss (particularly in the rostral territories), suggesting that collateral supply mainly proceeds from the anterior ICA’s branches in the ischemic neonatal mouse brain.
Analytical Investigation of Tension Loaded Deformed Rebar Anchors in Concrete
Authors: Chhetri Sandip , Chicchi Rachel A.
Published: June 2021

Experimental testing of deformed rebar anchors (DRAs) has not been performed extensively, so there is limited test data to understand their failure behavior. This study aims to expand upon these limited tests and understand the behavior of these anchors, when loaded in tension. Analytical benchmark models were created using available test data and a parametric study of deformed rebar anchors was performed. Anchor diameter, spacing, embedment, and number of anchors were varied for a total of 49 concrete breakout simulations. The different failure modes of anchors were predicted analytically, which showed that concrete breakout failure is prominent in the DRA groups. The predicted concrete breakout values were consistent with mean and 5% fractile concrete capacities determined from the ACI concrete capacity design (CCD) method. The 5% fractile factor determined empirically from the simulation results was kc = 26. This value corresponds closely with kc = 24 specified in ACI 318-19 and ACI 349-13 for cast-in place anchors. The analysis results show that the ACI CCD formula can be conservatively used to design DRAs loaded in tension by applying a kc factor no greater than 26.
Microbial-Driven Stabilisation of Archaeological Iron Artefacts
Authors: James Sarah , Joseph Edith
Published: June 2021

The instability of iron artefacts is rooted in salt contamination during burial and damages associated with exposure to alternative oxygen levels and high relative humidity once excavated. While a combination of chemical and mechanical treatments is utilised to remove the harmful ions (chlorides, sulphur species) and excess bulky corrosion products, these methods can be hazardous for conservation staff’s health, have limited success, or require extensive treatment times. Bio-based treatments provide a potentially greener alternative for removing damaging corrosion and creating biogenic mineral passivation layers, thus remediating concerns over costs, duration, and health and safety. Pseudomonas putida mt-2 (KT2440) is capable of utilising iron under certain conditions and for phosphating mild steel; however, applications have not been made in the cultural heritage sector. To address the potential of using bacteria for conservation purposes, Pseudomonas was assessed for both the bioremediation of salt contaminates and the production of a passivation layer suitable for iron artefacts, with specific conservation concerns in mind. Key factors for optimisation include the role of agitation, chloride content, and oxygen content on bacterial growth and biomineralisation. The initial results indicate a growth preference, not reliance, for NaCl and agitation with partial success of bioconversion of a mineral source.
Synthesis and Optical Characterizations of Yb3+: CaxSr1−xF2 Transparent Ceramics
Authors: Ling Hongran , Mei Bingchu , Li Weiwei , Yang Yu , Zhang Yongqiang , Liu Xinwen
Published: June 2021

In this study, 3 at.% Yb3+: CaxSr1−xF2 nanopowders were synthesized via the chemical co-precipitation method. Highly transparent 3 at.% Yb3+: CaxSr1−xF2 ceramics with various CaF2 concentrations were fabricated by hot-pressed sintering. The 3 at.% Yb3+: CaxSr1−xF2 nanopowders exhibited a spherical shape with slight agglomeration, and their particle size ranged from 26 nm to 36 nm. With an increase of the CaF2 concentration, the peak shape changed significantly and the width of the emission band increased inhomogeneously. The minimal fluorescence lifetime at the wavelength of 1011 nm of 3 at.% Yb3+: CaxSr1−xF2 transparent ceramics with various CaF2 concentrations was higher than 3.25 ms, which was longer than that of the 3 at.% Yb3+: CaF2 (2.6 ms) and the 3 at.% Yb3+: SrF2 (3.22 ms) reported in previous literature. The results indicate that incorporating Ca2+ ions into the SrF2 is an effective method to modulate the optical properties of transparent ceramics.
Emergency Use of Targeted Osmotic Lysis for the Treatment of a Patient with Aggressive Late-Stage Squamous Cell Carcinoma of the Cervix
Authors: Gould Harry J. , Miller Paige R. , Edenfield Samantha , Sherman Kelly Jean , Brady Chad K. , Paul Dennis
Published: June 2021

Upregulation of voltage-gated sodium channels (VGSCs) and Na+/K+-ATPase (sodium pumps) is common across most malignant carcinomas. Targeted osmotic lysis (TOL) is a developing technology in which the concomitant stimulation of VGSCs and pharmacological blockade of sodium pumps causes rapid selective osmotic lysis of carcinoma cells. This treatment of cervical carcinoma is evidence that TOL is a safe, well-tolerated and effective treatment for aggressive advanced carcinomas that has the potential to extend life without compromising its quality. TOL is likely to have broad application for the treatment of advanced-stage carcinomas.
Parrot Free-Flight as a Conservation Tool
Authors: Woodman Constance , Biro Chris , Brightsmith Donald J.
Published: June 2021

The release of captive-raised parrots to create or supplement wild populations has been critiqued due to variable survival rates and unreliable flocking behavior. Private bird owners free-fly their parrots in outdoor environments and utilize techniques that could address the needs of conservation breed and release projects. We present methods and results of a free-flight training technique used for 3 parrot flocks: A large-bodied (8 macaws of 3 species and 2 hybrids), small-bodied (25 individuals of 4 species), and a Sun Parakeet flock (4 individuals of 1 species). Obtained as chicks, the birds were hand-reared in an enriched environment. As juveniles, the birds were systematically exposed to increasingly complex wildland environments, mirroring the learning process of wild birds developing skills. The criteria we evaluated for each flock were predation rates, antipredator behavior, landscape navigation, and foraging. No parrots were lost to predation or disorientation during over 500 months of free-flight time, and all birds demonstrated effective flocking, desirable landscape navigation, and wild food usage. The authors conclude that this free-flight method may be directly applicable for conservation releases, similar to the use of falconry methods for raptor conservation.
Diversity of Algae and Cyanobacteria and Bioindication Characteristics of the Alpine Lake Nesamovyte (Eastern Carpathians, Ukraine) from 100 Years Ago to the Present
Authors: Tsarenko Petro M. , Bilous Olena P. , Kryvosheia-Zakharova Olha M. , Lilitska Halyna H. , Barinova Sophia
Published: June 2021

The species diversity and changes in the structural dynamics of the algal flora from the alpine lake Nesamovyte has been studied for 100 years. During the period of investigations, 234 species (245 infraspecific taxa) were revealed to cover more than 70% of the modern species composition of the studied lake. The modern biodiversity of algae is characterized by an increase in the number of widespread forms, a change from the baseline “montane” complex in comparison to the beginning of the 20th century. Nevertheless, the Nesamovyte Lake still has a unique algae composition that is typical for high-mountainous European lakes. The presence of a different complex of conventionally arctic species of algae, in particular, diatoms is discussed. Structural changes in the taxonomic composition of the algal flora of the lake as well as in the complex of the leading genera, species and their diversity are revealed. An ecological analysis of the algal species composition of the lake showed vulnerability and degradation to the ecosystem of the lake. On this basis, the issue regarding the question of protection and preservation of the algae significance and uniqueness of the flora of algae in the Nesamovyte Lake are discussed.
Local Information as an Essential Factor for Quantum Entanglement
Authors: Su Zhaofeng
Published: June 2021

Quantum entanglement is not only a fundamental concept in quantum mechanics but also a special resource for many important quantum information processing tasks. An intuitive way to understand quantum entanglement is to analyze its geometric parameters which include local parameters and correlation parameters. The correlation parameters have been extensively studied while the role of local parameters have not been drawn attention. In this paper, we investigate the question how local parameters of a two-qubit system affect quantum entanglement in both quantitative and qualitative perspective. Firstly, we find that the concurrence, a measure of quantum entanglement, of a general two-qubit state is bounded by the norms of local vectors and correlations matrix. Then, we derive a sufficient condition for a two-qubit being separable in perspective of local parameters. Finally, we find that different local parameters could make a state with fixed correlation matrix separable, entangled or even more qualitatively entangled than the one with vanished local parameters.
Domain-Specific Stimulation of Executive Functioning in Low-Performing Students with a Roma Background: Cognitive Potential of Mathematics
Authors: Kovalčíková Iveta , Veerbeek Jochanan , Vogelaar Bart , Prídavková Alena , Ferjenčík Ján , Šimčíková Edita , Tomková Blanka
Published: June 2021

The current study investigated whether a domain-specific intervention of ExeFun-Mat targeting math and executive functions in primary school children with a Roma background would be effective in improving their scholastic performance and executive functioning. ExeFun-Mat is based on the principles of the reciprocal teaching approach, scaffolding and self-questioning. The domain-specific content was divided into modules. Each module consisted of a set of graded tasks. The criteria for the grading and hierarchical organization of the tasks were based on the level of cognitive difficulty and the type of representation. In total, 122 students attending grade four of elementary school took part in the project. The study concerned a pretest-intervention-posttest experimental design with three conditions: the experimental condition, an active, and a passive control group. To assess the children’s level of EF, the Delis–Kaplan executive function system test battery was used; to assess children’s mathematical achievement, the cognitive abilities test (the numeracy battery), and ZAREKI—a neuropsychological test battery for numerical processing and calculation—were used. The results suggested that both math performance and executive functions improved over time, with no significant differences between the three conditions. An additional correlational analysis indicated that pretest performance was not related to posttest performance for the children in the experimental and active control group.
Oligothiophene-Naphthalimide Hybrids Connected through Rigid and Conjugated Linkers in Organic Electronics: An Overview
Authors: Alonso-Navarro Matías J. , Gala Elena , Ramos M. Mar , Ponce Ortiz Rocío , Segura José L.
Published: June 2021

In this article, we summarize the synthetic approaches developed in our research groups during the last decade to efficiently tune the optical, electrochemical and morphological characteristics of oligothiophene–naphthalimide assemblies. Different variables were tuned in these organic semiconductors, such as the planarity and the length of their π-conjugated backbones, the topology and energy levels of the frontier molecular orbitals (HOMO and LUMO) and their molecular dipole moments. The tuning of these properties can be connected with the microstructure properties observed by atomic force microscopy (AFM) and X-ray diffraction (XRD) in thin films as well as with the performances in organic field-effect transistors (OFETs). The possibility of incorporating these donor-acceptor assemblies into macromolecular structures is also addressed, and some innovative applications for these macromolecular systems, such as the degradation of organic pollutants in aqueous media, are also presented.
In-Situ Characterisation of Charge Transport in Organic Light-Emitting Diode by Impedance Spectroscopy
Authors: Chulkin Pavel
Published: June 2021

The article demonstrates an original, non-destructive technique that could be used to in situ monitor charge transport in organic light-emitting diodes. Impedance spectroscopy was successfully applied to determine an OLED’s charge carrier mobility and average charge density in the hole- and electron-transport layer in a range of applied voltages. The fabricated devices were composed of two commercially available materials: NPB (N,N′-di(1-naphthyl)-N,N′-diphenyl-(1,1′-biphenyl)-4,4′-diamine) and TPBi (2,2′,2″-(1,3,5-Benzinetriyl)-tris(1-phenyl-1-H-benzimidazole)) as hole- and electron-transport layers, respectively. By varying the thicknesses of the hole-transport layer (HTL) and the electron-transport layer (ETL), correlations between layer thickness and both charge carrier mobility and charge density were observed. A possibility of using the revealed dependencies to predict diode current–voltage characteristics in a wide range of applied voltage has been demonstrated. The technique based on a detailed analysis of charge carrier mobilities and densities is useful for choosing the appropriate transport layer thicknesses based on an investigation of a reference set of samples. An important feature of the work is its impact on the development of fundamental research methods that involve AC frequency response analysis by providing essential methodology on data processing.
Multi-Agent System Observer: Intelligent Support for Engaged E-Learning
Authors: Vuković Igor , Kuk Kristijan , Čisar Petar , Banđur Miloš , Banđur Đoko , Milić Nenad , Popović Brankica
Published: June 2021

Moodle is a widely deployed distance learning platform that provides numerous opportunities to enhance the learning process. Moodle’s importance in maintaining the continuity of education in states of emergency and other circumstances has been particularly demonstrated in the context of the COVID-19 virus’ rapid spread. However, there is a problem with personalizing the learning and monitoring of students’ work. There is room for upgrading the system by applying data mining and different machine-learning methods. The multi-agent Observer system proposed in our paper supports students engaged in learning by monitoring their work and making suggestions based on the prediction of their final course success, using indicators of engagement and machine-learning algorithms. A novelty is that Observer collects data independently of the Moodle database, autonomously creates a training set, and learns from gathered data. Since the data are anonymized, researchers and lecturers can freely use them for purposes broader than that specified for Observer. The paper shows how the methodology, technologies, and techniques used in Observer provide an autonomous system of personalized assistance for students within Moodle platforms.