bluuebunny/crossref_metadata_2025 huggingface dataset:
Biochemical property analysis of native probiotic isolates from selective poultry
Authors: Hossain KM , Das AK , Rahman SMM , Roy S , Hamid MA
Published: November 2021

Isolation and identification of probiotic bacteria are the prerequisites for their safer use in the food and feed industry. The objectives of the present study were the isolation of probiotic bacteria from the selective gastrointestinal tract of poultry obtained from Khulna and Barisal Divisions, and their identification based on bacterial morphological characterization and biochemical property analysis. Ten potential native probiotics were isolated from the poultry gastrointestinal tract and assayed for their morphological, physiological and biochemical properties. It was observed that, all the isolates were rod-shaped, gram-positive, endospore-negative, catalase-negative, non-motile and were able to ferment particular sugars which are an indicator for typical probiotic bacteria. The sugar fermentation pattern, ability to survive and growth in inhibitory substances like 1-4% NaCl, 0.3% bile salt as well as their ability to grow in different temperatures and pH levels ensured the presumptive identification of the lactic acid bacteria. All the ten isolates exhibited a clear zone of inhibition when they were grown with five enteric pathogens which are indicative of their antimicrobial activity. Ten isolates were assayed for their susceptibility to eight antibiotics using the disc diffusion method. All the isolates were resistant to tetracycline and nalidixic acid. Further research regarding molecular characterization and identification of specific genes using different technologies may open the door to utilize these isolates in different probiotic-based inventions.
Bang. J. Livs. Res. Vol. 27 (1&2), 2020: P. 39-54
Isolation, identification and biochemical characterization of lactic acid bacteria from selected yogurt samples
Authors: Amanullah SM , Kabir MA , Rahman MM , Halder P , Hossain SMJ , Samad MA
Published: November 2021

This research was conducted to study the types of lactic acid bacteria (LAB) present in selected yogurts available in the local market of Bangladesh. For this purpose, nine different yogurt samples were collected (viz. MV, Mw, Pst, Psr, Bik, Bog, WF, Kw and Nab) and cultured in the selective MRS agar media for enumerating LAB colony. Out of 9 samples, colony forming LAB were found in 6 samples and the population ranged from 1.0×104 to 9.5×105 cfu/ml. Catalase negative and Gram’s positive colonies were initially identified as LAB. Then the isolates were purified by subsequent culturing in MRS broth and MRS agar media. Biochemical properties of selected colonies were evaluated by performing gas production from glucose, growth at different temperatures (10ºC, 15ºC and 45ºC), growth at different NaCl concentrations (2, 4 and 6.5% NaCl) and sugar fermentation tests (lactose, sorbitol, salicin, trehalose, melibiose, sucrose, mannitol, melezitose, maltose, galactose, glucose, arabinose, raffinose and ribose). According to the tests stated above, a total of five different species of LAB were identified from 6 samples. The isolate Lactobacillus lactis was identified in MV, PSr and Bog yogurt, while Lactobacillus bulgaricus was found in MV and Bik yogurt. The species Leuconostoc cremoris, a avor producing bacteria, was found in six yogurt samples. On the other hand, Lactobacillus acidophilus and Lactobacillus helveticus was found only in Pst and Psr samples, respectively.
Bang. J. Livs. Res. Vol. 27 (1&2), 2020: P. 64-72
Effect of feeding high yielding fodders on growth performance of growing Hilly Brown Bengal goat
Authors: Rahman MM , Sarker NR , Alam MA
Published: November 2021

This study was carried out to evaluate the feeding effect of high yielding fodders (HYF) on feed intake and growth performance of growing Hilly Brown Bengal (HBB) goat. For this purpose, a feeding trail was conducted with 16 growing HBB kids (4 to 5 months) by dividing equally in four groups having four replicates for a period of 75 days. The goats in group T0 (control) received natural grass along with 101.30g concentrates and adlibitum cowpea hay, whereas in group T1, T2 and T3, only natural grass was replaced by BLRI Napier 3, BLRI Napier 4 and Ruzi fodder, respectively. Total dry matter intake (DMI), roughages DMI, crude protein intake (CP), body weight gain (kg), average daily gain (g) and forth night body weight gain (g/day) were studied. Results revealed that, DMI (288.39, 285.71, 293.48 and 301.35 g/day in group T0, T1, T2 and T3, respectively), body weight gain (3.60, 3.74, 3.73 and 3.77 kg in group T0, T1, T2 and T3, respectively), average daily gain (47.32, 49.16, 49.12 and 48.92 g in group T0, T1, T2 and T3, respectively) and feed conversion ratio (6.2, 5.9, 6.0 and 6.1 in group T0, T1, T2 and T3, respectively) of HBB kids were not differed significantly (P>0.05) for all the treatment groups. The DMI from roughages (196.2, 193.5, 201.3 and 209.2 g/day in group T0, T1, T2 and T3, respectively) and CP intake (38.7, 42.1, 48.3 and 38.8 g/day in group T0, T1, T2 and T3, respectively) were significantly varied (P<0.05), while highest CP intake was found in the treatment group T2. Forthnight body weight (g/day) was not significantly (P>0.05) gained with feeding of HYF for all the treatment groups. From this study, it can be concluded that, growing HBB kids could equally be supplied with either BLRI Napier 3 or BLRI Napier 4 or Ruzi fodder in addition to ad libitum cowpea hay for better voluntary feed intake and growth.
Bang. J. Livs. Res. Vol. 27 (1&2), 2020: P. 73-81
Ovarian physiology of repeat breeder cows at Bathan area of Bangladesh
Authors: Ali MY , Ershaduzzaman M , Khatun R , Alam US , Akter S , Roy RR , Habib MA , Munira S , Esha JM , Sarker NR
Published: November 2021

The experiment was implemented at farmer’s house and Bathan areas of Shahjadpur Upazila in Sirajganj and Bera Upazila under Pabna districts of Bangladesh. The aim of the study was to know the incidence of repeat breeding (RB), physiology of different follicles and deformity of ovary and uterus in Holstein Frisian (HF) cows. Forty (40) RB HF cows (>50% HF Genetic) were selected on the basis of age and parity. Pyometra, endo-metra, cervicitis, Salpingitis and tumor like growth etc. in uterine wall were observed. Luteal and follicular cyst was also identified. Number of growing follicle appeared ultrasonically, size of Graafian follicle, breed, cow’s body size, and inheritance level and parity, nutritional factors and days calving to first service were recorded properly. All collected data were statistically analyzed by the SPSS soft-ware program using completely randomized design (CRD). Results revealed that, significantly (P<0.05) highest Body Condition Score (BCS) was 3.47±0.05 observed in the cows of group-D whose age were more than 8 years of old compared to others group (table 1). In-case of average daily milk yield and remained dry condition differences were highly significant (P<0.001). Maximum milk yield (20.41±0.82 l/day) was observed in group-C compared to others group but minimum dry condition remaining period was observed in group-A than others. Days of heat showed number of AI service were non-significant (P>0.05) in the whole experiment (table-1). The percentage of infection (Pyometra, Endometra) in the uterine wall differed non-significantly (P>0.05) among all the groups and minimum occurrence were observed in group-A (figure 1). Highest percent of cyst observed in the right ovary than left of the cows of group-D compared to others group but the differences were non-significant (P>0.05).Comparatively higher percentage (58.82±12.30) of follicles observed in left ovary of group-D than right ovary of others group (table 3). Non-significantly (P>0.05) the highest average number (2.40±0.44) of follicles was observed in left ovary of the group-C than the cows of others group. Though the follicle size in the end of luteal phase differed non-significantly (P>0.05) but comparatively larger (14.90±1.54mm) follicle in diameter were observed in group-C, than the follicles of others groups.
Bang. J. Livs. Res. Vol. 27 (1&2), 2020: P. 95-104
Prevalence and molecular detection of infectious laryngotracheitis virus in chickens in selected areas of Bangladesh
Authors: Ali MZ , Giasuddin M
Published: November 2021

Infectious laryngotracheitis (ILT) is a viral disease of poultry species caused by infectious laryngotracheitis virus (ILTV) that shows high morbidity and mortality. The present study was under taken for ILTV prevalence in broiler and layer chickens from four different geographical areas including Bogura, Gazipur, Chattogram and Dhaka districts during 2017 to 2018. Total 350 tracheal swabs were collected and were evaluated by real time RT-PCR (rRT-PCR). The overall 5.14% (18/350) ILTV prevalence was found that included 6.5% (13/200) in layer and 3.33% (5/150) in broiler chickens. The prevalence of ILTV was highest (10%) in layer chickens under age below 20 weeks and broiler chicks showed ILTV (1. 42%) infection when they were 7-14 days old. Winter season showed highest 6.6% prevalence whereas 5% and 3% prevalence were noticed at summer and rainy seasons, respectively.
Bang. J. Livs. Res. Vol. 27 (1&2), 2020: P. 113-117
Effects of Diaphragmatic Mobilization and Diaphragmatic Breathing Exercises on Pain and Quality of Life in Individuals with Shoulder Pain: A Randomized Controlled Trial
Authors: Şahin Okan , Kocamaz Deniz
Published: November 2021

Abstract

Purpose: This study aimed to investigate the effects of diaphragmatic mobilization and diaphragmatic breathing exercises on pain and quality of life in individuals with shoulder pain. Methods: A total of 72 individuals with shoulder pain were included in the study. The individuals included in the study were randomly divided into 3 groups as Diaphragmatic Mobilization Group (DMG) (n=24), Diaphragmatic Breathing Group (DBG) (n=24), and control group (n=24). In addition to classical physiotherapy treatment, diaphragmatic mobilization to mobilization group and diaphragmatic breathing exercises to breathing group were applied by the physiotherapist 3 days a week for 8 weeks. On the other hand, only classical physiotherapy treatments were applied to the control group. Individuals were evaluated in terms of pain and quality of life. Pain levels assessed with the Visual Analogue Scale (VAS) and quality of life assessed with Short Form-36 (SF-36). Results: In our study, it was observed that the pain level was reduced and the quality of life was improved in all groups (p<0.05). When DMG and DBG were compared with the control group, there was found a significant difference among groups in terms of reducing pain and improving quality of life in favor of DMG and DBG (p<0.05). When DMG and DBG were compared, no statistically significant difference was found between the groups (p>0.05). Conclusion: The study results demonstrated that diaphragmatic mobilization and diaphragmatic breathing exercises are found to be effective in reducing pain and improving quality of life in individuals with shoulder pain.
Comparison of the Supervised and Home-Based Physiotherapy Program in Patients with Lateral Epicondylitis
Authors: Şavkın Raziye , Şimşek Şule
Published: November 2021

Objective: This study aimed to compare the effectiveness of a supervised and home-based physiotherapy program in the reduction of pain and in improving functional status and quality of life in patients with lateral epicondylitis. Method: Patients diagnosed with lateral epicondylitis and referred to the physiotherapy clinic were evaluated retrospectively. A total of 32 patients were separated into two groups as supervised and home-based physiotherapy groups. All the patients received standard physiotherapy program consisting of static stretching exercises of the ECRB tendon, eccentric exercises of the wrist extensors and cold application, for 3 weeks. The groups were compared in terms of pain severity, range of motion, functional status (Quick-DASH) and quality of life (SF-36) after treatment. Results: Fifteen patients (12 females, 3 males) with a mean age of 44.20±7.35 years were included in the supervised physiotherapy group, and 17 patients (15 females, 2 males) with a mean age of 48.65±12.33 years in the home-based physiotherapy group. There was no statistically significant difference in activity (p=0.980) and rest pain (p=0.483), wrist flexion (p=0.775) and extension (p=0.838) range of motion, functionality (p=0.346) and quality of life (p=0.923) between groups. Conclusion: Patients participating in a home-based physiotherapy program had similar clinical outcomes in terms of pain intensity, range of motion, functionality, and quality of life as patients participating in a supervised physiotherapy. Further prospective randomized studies can be conducted by increasing the sample size and comparing with cost analyzes to determine the rehabilitation regimen that will provide the best outcomes in lateral epicondylitis rehabilitation.
Non-conventional therapies for analgesia
Authors: Pellizoni Mariana Papa , Faccio Rafael , Jodas Cláudio Roberto Pacheco , Grilo Ricardo , Teixeira Rubens Gonçalves , Leal Marilia de Oliveira Coelho Dutra
Published: November 2021

Purpose: Third molar surgery is a quite common procedure. Some patients experience pain associated with this situation. The aim of this article aims to discuss non-conventional therapies for the management of postoperative pain associated with third molar surgery. Methods: An extensive literature search was conducted with the keywords “third molar” + “pain” and all articles that excluded already established drugs as analgesics, anti-inflammatories, or therapies such as laser therapy and ozone therapy whereas non-conventional drugs and therapies were retained. Results: The article rated bromelain, honey, hyaluronic acid, nicotine patches, Kinesio tape, TENS, anticonvulsants, curcumin, and melatonin. Some therapies are efficient and safe, while others have not shown promising results. Conclusions: Bromelain and Kinesio tape are considered the most effective non-conventional therapies for controlling postoperative pain, and even with few studies on them, they are therapies with promising results.
Use of virginiamycin improves performance of high-prolific sows during gestation and lactation over two cycles
Authors: Silva Bruno Alexander Nunes , Santos Leonardo David Tuffi , Lanna Dante Pazzanese Duarte , Kippert Carlos Junior , Campos Paulo Henrique Reis Furtado , Araujo Wagner Azis Garcia , Silva Kariny Fonseca da , Cardoso Hysla Milena Cunha
Published: November 2021

Feed additives can be used as potential strategies to enhance the efficiency of nutrient utilization by the sow can impact on the reduction of feed costs and in addition improve sow and litter health. The objective of this study was to evaluate the impact of the supplementation of virginiamycin (VM) in diets for gestating and lactating sows over two consecutive productive cycles on the animals productive and reproductive performance. Sows that received VM showed a lower BW, body protein and energy losses during both lactations when compared to control sows (P = 0.001). VM treatment increased (+6%; P = 0.001) piglet weaning weight. The litter daily gain was also influenced (P = 0.001) by the treatments were litters from VM fed sows showed an improved daily gain when compared to control sows (2.61 vs. 2.24 kg d-1 respectively). Average daily milk production improved by 17% (P = 0.001) in the VM sows when compared with the control. Also, an improvement in milk fatty acid profiles were found in sows fed VM. As a conclusion, we can infer that the constant use of VM in the diets of sows during gestation and lactation improves both sow and litter performance.
Avaliação da ocorrência de doenças periodontais entre os pacientes atendidos em uma clínica escola de odontologia de um centro universitário do sudoeste da Bahia
Authors: Macedo Lizandra Gomes , Dias Karina Sarno Paes Alves
Published: November 2021

O presente estudo tem como objetivo determinar a ocorrência da doença periodontal entre os pacientes atendidos identificando quais doenças periodontais são mais prevalentes e observando os possíveis fatores de risco a partir dos dados obtidos através da análise dos prontuários. Na pesquisa nessa mesma clínica escola, dos sexos feminino e masculino, com idades entre 22 a 73 anosOs dados coletados foram tabelados em um banco de dados elaborado no Microsoft Excel (Microsoft Corporation, USA), onde foram tabulados o número de pacientes, sexo, idade, etnia, presença ou ausência de doença sistêmica relatada pelo paciente através da anamnese presente em seu prontuário, data do exame periodontal e diagnóstico periodontal. A análise estatística foi realizada através dos testes de Qui-quadrado, Mann-Whitney e Kruskal-Wallis. De acordo com os resultados logrados nesta pesquisa, observou-se que há alta ocorrência de doenças periodontais, comprovando a relação da doença com a condição sistêmica do paciente. Os dados avaliados evidenciaram um alto índice da ocorrência de doenças periodontais, identificando a mais prevalente, pontuando os fatores de risco e correlacionando-as com doenças sistêmicas.
Evaluation of culture media and conditions of Amazonian filamentous fungi in an antimicrobial screening program
Authors: Negreiros Monique Antunes , Guedes Milthes Viana , Rodrigues Thaís Karoline de Sousa , Rodrigues Beatriz Rebelo , Cruz Andria Lopes , Rosa Priscila Dallé da , Andrade Saulo Fernandes de , Fuentefria Alexandre Meneghello , Campos Ranieri , Abegg Maxwel Adriano
Published: November 2021

The rediscovery of bioactive compounds is a problem within natural product screening programs, because the chemical-genetic diversity of fungi is little explored and the standardization of cultivation conditions that allow obtaining new actives is critical in such programs. In this work, the impact of two solid mediums (rice and oats), a liquid medium (Czapeck broth) and different fermentation conditions were evaluated in order to explore new metabolic routes. Twelve filamentous fungi from Amazonian environments were used. UV-Vis spectrophotometry estimated the complexity of the extracts produced. The antimicrobial activity of the extracts was evaluated against an isolate of each Escherichia coli strain, Salmonella sp. and Staphylococcus aureus. Solid media proved to be more promising, as they allowed a wider range of active metabolites to be obtained. The oat medium provided a greater variety of metabolites, but due to the great complexity of the extracts obtained, the separation procedures were considerably more complex than for rice. Together, the rice culture medium and the use of 39 days of fermentation proved to be more promising conditions than the liquid medium normally used in screening programs in Brazil. The cultivation of Penicillium maximae (isolated for the second time in Brazilian territory) in solid medium provided the production of active fractions against E. coli in bioautography. In this study, it was observed that different fermentation conditions in solid culture are considerably promising in the search for bioactive natural products.
Resveratrol-supplemented holding or re-culture media improves viability of fresh or vitrified-warmed in vitro-derived bovine embryos
Authors: Silva Arianny Rafaela Neto , Marques Thaisa Campos , Santos Elisa Caroline Silva , Diesel Tiago Omar , Macedo Isabelle Matos , Teixeira Rafaela Cavalcanti , Martins Carlos Frederico , Alves Benner Geraldo , Gambarini Maria Lucia
Published: November 2021

The effect of resveratrol supplementation on fresh (E1) or vitrified/warmed (E2) in vitro produced bovine embryos was investigated by evaluating the time-dependent response. After in vitro production, resveratrol (0.5 µM) was added to the incubation media and after two incubation periods with or without resveratrol, blastocysts were re-cultured for 24h. The rates of re-expansion, hatching, total cell number (TCN), apoptotic cells (ACN), reactive oxygen species (ROS) and intracellular glutathione (GSH) content were evaluated. For E1, the re-expansion rate differed at 6 and 10h within and between treatments (P<0.05), as did the re-expansion rate after 24h (P<0.01). The hatching rate increased after 10h with resveratrol (P<0.01) with differences within (P<0.05), but not between treatments after 24h of re-cultivation. At E2, hatching rate differed between treatments at 24h (P<0.01), with higher TCN in resveratrol-treated blastocysts after 10h (P<0.01). Resveratrol supplementation reduced ROS generation in E1 and E2 after 10h of incubation and increased GSH content (P<0.01). These results indicate that supplementation of holding re-cultivation medium with resveratrol for treatment of fresh or vitrified/warmed in vitro produced bovine embryos has a positive and time-dependent effect. The reduction of ROS content, the increase of GSH and the anti-apoptotic ability of resveratrol are responsible for its protective effects, allowing an extension of embryo storage time before transfer to recipients.
Haff disease in the pandemic COVID-19 period in Brazil
Authors: Campos Gabriella de Almeida , Dantas Sabrina Jurema da Rocha , Carvalho Emanuel Guilherme de Almeida , Tabosa Rafael Vinicius de Almeida , Souza Elvys Blayne Sales de , Lino Andrezza Thaís da Silva , Leite Magnúcia de Lima , Biase Carmem Lúcia Carneiro Leão de , Leão Sura Amélia Barbosa Felix , Felix Valtuir Barbosa , Trindade-Filho Euclides Maurício , Silva José Claudio da
Published: November 2021

Objective: This study describes and characterize the cases of Haff disease reported in Brazil during the COVID-19 pandemic period. Descriptive and cross-sectional study using public data from the National Center for Strategic Information and Response in Health Surveillance nacional. The Risk Communication number 17 of 09/15/2021 was accessed. The description of suspected cases was extended to all Brazilian states where notifications occurred, from January 1, 2020, to September 15, 2021. There was an increase in cases of Haff's disease in Brazil, from 2020 onwards, with 46 in the Northeast region of Brazil. Eighty-five cases and three deaths were reported between January and September 2021 in the North, Northeast, South and Midwest regions. Most cases occurred in regions of greater heat such as the coastal region of the Northeast and around regions of the Amazon River Basin. A critical period for coping with the SARS-CoV-2 virus pandemic in Brazil, cases of disease of Haff could be reported in various regions of country. Despite the etiological suspicions, studies, and follow-up of the cases, it is still not possible to affirm that the disease is caused by biomolecules of the Palytoxin-Like Toxins group. The possible identification of the responsible toxin(s) will be essential for a complete understanding of the etiopathogenic mechanism of the disease and adequate development of prevention, treatment, and cure measures.
Capital Social e Desenvolvimento Local: Contribuições do Conselho de Desenvolvimento Econômico e Social de Nova Serrana, Nova Serrana, Minas Gerais
Authors: Barbosa Rodney Alves , Vilela Katia de Fatima , Lacerda Marco Aurélio
Published: November 2021

Dentro de uma sociedade, o capital social está nas estruturas das redes sociais, que, por sua vez, estabelecem relações duráveis entre os atores, por meio de preceitos como reciprocidade e confiabilidade, o que facilita ações como a formação de conselhos que buscam recursos coletivos para a comunidade. O objetivo deste estudo, foi identificar as ações do Conselho, os sentidos da participação social e suas implicações na construção de um modelo participativo-democrático. A metodologia utilizada baseia-se no estudo de caso do Conselho de Desenvolvimento Econômico e Social de Nova Serrana – MG. Foram realizadas análises documentais e entrevistas semiestruturadas. Como resultados, foi possível constatar que as ações do Conselho estão ligadas às áreas de saúde, segurança, educação, infraestrutura e articulação entre os Poderes Executivo e Legislativo. Conclui-se que Conselho é um importante instrumento de ampliação da liberdade participativa-democrática, o que reforça a participação social efetiva dos atores sociais. Na mesma medida, ele promove e fortalece o capital social. O conjunto dessas ações tem resultado no fortalecimento do próprio Conselho e no desenvolvimento econômico e social do município.
Community Perceptions and Environmental Conditions Around the Tegalgondo and Kepuharjo Temporary Trash Shelter Sites, Karangploso Sub-District
Authors: Indriyana Nunuk Setia , L Ratna Djuniwati , Laili Saimul
Published: November 2021

Community behavior in disposing of waste plays an important role in improving public health. Community participation is needed to reduce the amount of waste, especially the role of housewives. Efforts to reduce the amount of household waste require support, one of which is the public perception of household waste processing. So far, people do not have the habit of sorting and selecting waste so that the amount of waste disposed of at TPS continues to increase. This increase can affect the environmental conditions around the TPS if there is an accumulation of waste in the long term. This study aims to analyze public perceptions of TPS Tegalgondo and TPS Kepuharjo and to analyze the environmental conditions around TPS Tegalgondo and TPS Kepuharjo in Karangploso District. The method used in this research is descriptive quantitative and direct field surveys. Based on the results of research conducted. The results of public perceptions of TPS Kepuharjo got very high perceptual scores about household waste management and low perceptions about TPS. The results of the analysis of environmental conditions at TPS Tegalgondo for the total risk value is 170 which is included in the medium category which means it requires high level management attention. The results of the analysis
Uji Toksisitas Subkronik 28 Hari Kombinasi Ekstrak Metanolik Benalu Teh dan Benalu Mangga terhadap Profil Protein pada Tikus Wistar (Rattus norvegicus) Betina
Authors: Kinasih Umu Intan , Abdoes Sjakoer Nour Athiroh , Mubarakati Nurul Jadid
Published: November 2021

There are 3 indicators for indicating a protein breakdown in the blood through a total protein, albumin, and globulin stability. A compound capable of acting as antioxidants is needed to stabilize the protein's condition. This research is aimed at identifying the toxic effects of extract’s combination from tea parasite and mango parasite on the conditions of blood’s protein by giving EMBTBM constantly for 28 days. The data analysis used ANOVA one-way by SPSS version 17.0. As for the animal test used was a female Wistar rat of 20 with four treatments are control, treatment 1 with a dose of 250mg/Kg body weight (BW). Treatment 2 with a dose of 500mg/Kg body weight (BW) and treatment 3 with a dose of 1000mg/Kg body weight (BW). The result of this research shows that there is no difference between treatment and control over the results of a total protein and albumin. But, at the result of globulin, there is a difference between treatment and control. Therefore, EMBTBM is safe for profile protein and does not cause toxicity because protein is stable and not over-reducing.

 

Keywords:Subchronic,Total Proteins, Albumine, Globuline, Toxicity
Ekstraksi Astaxanthin Kulit Udang (Litopenaeus vannamei) Pantai Gunung Kidul Menggunakan Pelarut Minyak Bunga Matahari dan Etanol
Authors: Prasetyaningsih Anik , Najoan Graciela Carina , Wisaksono Abner , Rahardjo Djoko
Published: November 2021

ABSTRACT

 

As a maritime country with vast waters, Indonesia has many opportunities to utilize marine resources as a source of bioactive compounds that have the potential as active medicinal ingredients. One of the marine biotas that potentially contains the active compounds is the Vannamei shrimp's shell (Litopenaeus vannamei), which is commonly found as waste along the coast of Gunungkidul, Yogyakarta. The shrimp’s shell contains astaxanthin, a potential source of antioxidants for the health industry. The purpose of this study was to compare the astaxanthin extraction yield from L. vannamei shrimp shells using sunflower oil and 70% ethanol. The Astaxanthin extraction used sunflower oil and ethanol 70% as solvents and was done by maceration method, while the phytochemical test and Astaxanthin profiling used Thin Layer Chromatography and Spectrophotometer with Kelly and Harmon (1972) [5] calculations as well as pure Astaxanthin standards. The extraction yield of the 70% ethanol extraction was further processed by column chromatography using ether: ethanol (8: 2) as mobile phase. The highest Astaxanthin yield (220 mg / g of shrimp powder) was obtained from the extraction with sunflower oil compared to the 70% ethanol solvent, while the fractionation result with a chromatographic column from a crude extract of ethanol 70% showed high astaxanthin yield of 220.77 mg. / g fraction. The results of the fraction test on rat neutrophils, the best percentage reduction was at a concentration of 150 mg / g bw of rats.
Studi Nilai Etnobotani dan Sosio-Ekologi Generasi Milenial Sumba
Authors: Istikomayanti Yuswa , Mitasari Zuni
Published: November 2021

Ethnic and natural environments coexist together. However, along with the development of modernization to various corners of the region also makes challenges for indigenous peoples to remain in the future. The purpose of this study is to describe ethnobotany values ​​in the Sumba millennial generation and to examine the socio-ecological aspects as a new perspective in ethnobotany studies. Sumba millennials are students of the Tribhuwana Tunggadewi University who are regional children who still live with a cultural environment, especially the Sumba area, East Nusa Tenggara. Through questionnaires, open questions and in-depth interviews with key respondents, it can be assessed the extent to which cultural values ​​exist in the Sumba millennial generation who have received education and modern life. Socio-ecological studies are important to be carried out as a perspective in making various policy directions. Particularly in the direction of education, selection, and determination of customary values ​​and how efforts to conserve them become co-existent ethnicities and environments. The results obtained by most of the millennial generation still hold customary values ​​in their daily behavior. This aspect is reflected in the use of traditional medicine, skills in weaving, natural coloring, eating betel-nut, and positive perceptions of the preservation of its culture in the future. 

Keywords:Ethnobotany, Indonesia, Milenial, Sumba, Socio-Ecology

ABSTRAK

Etnis dan lingkungan alam hidup berdampingan. Namun, dengan seiring berkembangnya modernisasi hingga ke berbagai pelosok daerah juga menjadikan tantangan untuk masyarakat adat tetap bertahan di masa depan. Tujuan penelitian ini untuk mendeskripsikan nilai-nilai etnobotani pada generasi milenial Sumba serta mengkaji aspek sosio-ekologi sebagai perspektif baru dalam kajian etnobotani. Milenial Sumba yaitu mahasiswa Universitas Tribhuwana Tunggadewi yang merupakan anak daerah yang masih hidup dengan lingkungan kebudayaan khususnya daerah Sumba, Nusa Tenggara Timur. Melalui kuesioner, pertanyaan terbuka serta wawancara mendalam pada responden kunci dapat dikaji sejauh mana nilai-nilai kebudayaan yang ada pada generasi milenial Sumba yang telah mengenyam pendidikan dan kehidupan modern di Kota Malang, Jawa Timur, Indonesia. Kajian sosio-ekologi menjadi penting untuk dilakukan sebagai perspektif dalam pengambilan arah berbagai kebijakan. Khususnya dalam arah pendidikan, pemilihan, dan penentuan nilai-nilai adat dan bagaimana upaya pelestariannya menjadi etnis dan lingkungan yang saling berdampingan. Hasil yang diperoleh sebagian besar generasi milenial masih memegang nilai adat dalam perilakunya sehari-hari. Aspek tersebut tercermin dalam penggunaan obat tradisional, keterampilan menenun, mewarnai secara alami, makan sirih-pinang, serta persepsi positif terhadap kelestarian budayanya di masa depan.

Kata kunci: etnobotani, Indonesia, milenial, Sumba, Sosio-Ekologi
Studi Etnobotani Kunyit (Curcuma) Pada Masyarakat Desa Klabetan Kecamatan Sepulu Kabupaten Bangkalan Madura Jawa Timur
Authors: Rohmah Garnis Mufarrohah , Hayati Ari , Rahayu Tintrim
Published: November 2021

Tanaman kunyit(Curcuma) relatif mudah ditemukan dan penggunannya sudah meluas tidak hanya untuk keperluan memasak namun juga untuk kesehatan. kunyit (Curcuma) atau disebut Konyek dalam Bahasa Madura adalah tanaman yang sudah sering dimanfaatkan oleh masyarakat Desa Klabetan sebagai bahan pangan maupun obat tradisional yang sering disebut Jhemoh dalam Bahasa Madura. Secara umum minum jamu diracik dari tumbuhan telah terjadi mulai sejak turun-temurun oleh masyarakat Madura. Penelitian bertujuan untuk mengetahui persepsi masyarakat tentang pemanfaatan kunyit (Curcuma) di Desa Klabetan kecamatan Sepuluh kabupaten Bangkalan. Menggunakan metode deskriptif esploratif: studi pustaka, pengamatan di lapang, wawancara, analisis data dan dokumentasi persebaran tanaman kunyit (Curcuma) di Desa Klabetan Kecamatan Sepuluh Kabupaten Bangkalan. Hasil penelitian menunjukkan terdapat dua karakteristik kunyit yaitu kunyit (Curcuma) dengan kunyit pada pada organ sebagai bahan pangan 51% , obat obatan 46% dan ritual adat 3%. Bagian organ tanaman (Curcuma) jumlah daun 38%, rimpang 50% batang 12%. Jumlah kunyit (Curcuma) yang ditemukan sebanyak 7 titik di Dusun Bindeng dan 6 titik Dusun Bilarangan dari tiga Dusun.
Ethnobotany Study of Corn (Zea mays. L) in Tamberu Village West Sokobanah Subdistrict Sampang Madura District
Authors: Mufidah Kamiliatul , Hayati Ari , Zayadi Hasan
Published: November 2021

Corn, which is a tropical grass that is very adaptive to climate change and corn, also has a life span of 75-150 days. Corn can usually grow to reach a height of 3 meters. Corn which usually has a scientific name Zea mays is not like other grain plants, Corn and commonly called jhegung in the language of Madura is a plant that has often been made into cultivated plants and has often been used by the community groups of West Tamberu Village as food as fuel or as animal feed. This study aims to determine the public perception of the benefits of corn plants found in Tamberu Barat Village, Sokobanah District, Sampang Madura Regency. In this study, researchers used descriptive exploratory methods which included: first-hand study, direct observation in the field, interviews using questionnaire guidance, data analysis and documentation of utilization of corn plants. The characteristics of the varieties found in Tamberu Barat Village consist of three varieties, namely local maize plants, hybrid maize plants, sweet corn plants. Public perceptions of corn plants in the western tamberu village are very high potential of corn plants which are used as food (59%), as fuel (10%), as animal feed (13%) and economic needs (18%).
The Effect of Exposure of Cigarette Smoke With Herb Additives on Leukocyte and Lung Histopathology of Mice (Mus musculus)
Authors: Ardiansyah M Luthfi , Sukmaningsih A.A.S.A , Narayani Inna
Published: November 2021

Smoking habits have been around since ancient times, but nowadays this habit is considered to be detrimental, especially to health. The impact that is often felt by smokers is difficulty in breathing because the lungs are exposed to cigarette smoke. Cigarette smoke contains about 1015-1017 oxidants or free radicals, as well as 4700 harmful chemicals, including aldehydes / carbonyls, NO2, and SO2. Herbal cigarettes are tobacco cigarettes with added ingredients from plants. Gurah terapi sin cigarettes are herbal cigarettes that are sold commercially. The aim of this study was to determine the effect of gurah cigarette smoking on the leukocytes and lung histology of mice. This study used a comparative method consisting of 3 groups, namely the control was not exposed to cigarette smoke, treatment 1 was exposed to commercial cigarette smoke and treatment 2 was exposed to cigarette smoke with herbal ingredients and each group consisted of 10 replications. The results showed that there were significant differences (p <0.05) regarding the number of cell necrosis, type II pneumocytes, inflammatory cell infiltration, hemorrhage, and alveolar dilation. While the results of the analysis of the number of leukocytes showed no significant difference where p > 0.05. The results showed that there was no significant difference in the number of leukocytes in the control group, treatment 1 and treatment 2 (p > 0.05). herbs containing various kinds of antioxidants cause a tendency for differences in the number of leukocytes where there is a decrease in the number of lymphocytes and neutrophils and an improvement in the histopathological structure of the lung against type I pneumocyte cell necrosis, hemorrhage, alveolar dilation, type II pneumocyte cell proliferation, and inflammatory cell infiltration in exposed mice. commercial cigarette smoke without herbal ingredients.
Pengaruh Perbedaan Konsentrasi Emulgator terhadap Karakterisasi Fisik Sediaan Krim Ekstrak Rosella (Hibiscus sabdariffa)
Authors: Widyaningrum Ike , Purwanti Sasi
Published: November 2021

The formulation and physical characterization test of rosella extract cream preparations have been carried out using various types of non-ionic emulsifier concentration ratios (combination of tween 80 and span 80). Comparison of the concentrations of tween 80 and span 80 used in this study were 2%, 3%, and 4%. The aim of this study included organoleptic test, homogeneity test, pH test, viscosity test, dispersion test, and emulsion type test. The result obtained from the physical characterization test were the organoleptic tests for the three formulas showed the same organoleptic results, namely red color, characteristic smell of rosella, and semisolid consistency. Homogeinity test shows that the formula is homogenous. The pH test showed that F1 4,29; F2 4,6; F3 5. Viscosity test showed that F1 432,4 mPas; F2 434,3 mPas; 435,3 mPas. The dispersion test was carried out without using a load of 50 g, 100 g, and 200 g. the results shown form the spreadability test of three formulas were were F1 5 cm; 5,5 cm; and 6 cm; F2 6 cm; 6,35 cm; 7,25; and 7,5 cm. F3 6,25 cm; 7,5 cm; 7,75 cm; 8 cm. The emulsion type indicated by the three formulas is oil in water. Based on these results, it can be seen that the emulsifier concentration has an effect on PH, viscosity, and spreadibility. The higher the concentration of emulsifier used, the higher pH value, viscosity, and spreadibility.
Features of the current epizootic situation and the epidemiological situation for plague in southern Siberia, Mongolia and China (review)
Authors: Balakhonov S. V. , Verzhutsky D. B. , Korzun V. M. , Kulikalova E. S. , Kholin A. V. , Sharakshanov M. B.
Published: November 2021

The purpose of this article is to present data on the current plague situation in China, Mongolia and southern Siberia (Russia). The analysis of the plague epizootic situation in the natural foci of the Central Asian region has been carried out. An increase in the activity of epizootics in a significant part of the territory under consideration and epidemiological risks rise were shown, which led to a sharp escalate in the number of plague diseases in people, including fatal outcomes. The extremely dangerous epidemiological situation emerging in the region dictates the need to continue systematic investigations of natural foci of plague in southern Siberia, to constantly monitor the epidemiological situation in Mongolia and China, to carry out comprehensive preventive and anti-epidemic measures to reduce the risks of plague in humans and to exclude the possibility of anthroponous spread of infection.
Using molecular markers to study epidemiologically important trematode representatives, Clonorchis sinensis and Metagonimus suifunensis, in the Russian Far East
Authors: Shumenko Polina Gennadievna , Tatonova Yuliya Viktorovna
Published: November 2021

Despite the epidemiological significance of the trematodes Clonorchis sinensis and Metagonimus suifunensis, these species are insufficiently studied in the Russian Far East. In this work, we compare various nuclear and mitochondrial markers used for population genetic studies, based on previously published articles, as well as new data on the nad1 mtDNA gene obtained for M. suifunensis. Even such conservative markers as the ITS1–5.8S-ITS2 rDNA region and the cox1 mtDNA gene are suitable for the analysis of genetic variability in the C. sinensis population, while more variable mtDNA genes must be used to assess the population structure of M. suifunensis. However, the level of variability is not the only determining factor when choosing a marker. The nad1 mtDNA gene did not reveal patterns within the M. suifunensis population, like the cox1 gene, while the mtDNA cytb gene, with lower variability indices, showed a geographical subdivision and migration ways for individuals of the species.
Investigation of the ability of Francisella tularensis strains to form biofilms
Authors: Naumova Kseniya Viktorovna , Mazepa Andrey Vladimirovich , Syngeeva Ayuna Konstantinovna , Kulikalova Elena Stanislavovna
Published: November 2021

The study results of the Francisella tularensis stains’ ability to form biofilm on the substrate surface are presented. The qualitative analysis of biofilm forming in the different culture conditions at the surface of glass vials has been performed. It was shown that all studied strains could form a biofilm, but most optimal conditions for biofilm formation are temperature at 22 ± 1 °C and culture exposition during 192 hours.
The ability of Salmonella enterica to biofilm formation in conditions of interspecific interaction with representatives of the intestinal microflora
Authors: Matosova E.V.
Published: November 2021

For effective distribution in the gastrointestinal tract, Salmonella must have the ability to form biofilms and compete for nutrients with the host microbiota. Objective: to characterize the ability of Salmonella enterica Typhimurium to biofilm formation under conditions of interspecific interaction in a multicultural biofilm with representatives of the intestinal microbiota in an in vitro experiment. Museum strains of the bacteria Salmonella enterica serovar Typhimurium, Lactobacillus acidophilus and Lactobacillus casei, Escherichia coli were used in the work. The process of interspecific interaction was modeled in a mixture of LB-broth with 0.85 % NaCl solution in a ratio of 1:3 at an initial bacterial concentration of 10³ CFU / ml in Petri dishes with a diameter of 65 mm at a temperature of +37 °C. The experimental results were evaluated within 12 days. The plate method was used to count the number of viable cells in biofilms. The optical density of the matrix of the biofilm stained with gentian violet was measured using spectrophotometry (wavelength 595 nm). To assess tinctorial properties, the bacterial samples were Gram-stained.. Enzymatic properties were assessed using Giss media and differential diagnostic media. All experiments were repeated 3 times. It was found that Salmonella enterica Typhimurium exhibit the ability to form biofilm. In the initial stages of biofilm formation, the bacteria of the intestinal microbiota suppress the development of Salmonella. In mature mixed biofilms, the growth of S. enterica and E. coli is mutually stimulated. By the end of the experiment, the dominance of Salmonella over the intestinal microbiota was noted. The results of the optical density measurement suggest the presence of species-specificity in bacterial interaction. Conclusion. Intestinal microbiota bacteria inhibit the growth of Salmonella bacteria in biofilms only in the initial stages. In mature mixed biofilms, the growth of E. coli and S. enterica is mutually stimulated, however, on the 12th day, Salmonella dominates over E. coli and Lactobacillus spp. (p > 0.05).

English version of the article is available at URL: https://panor.ru/articles/ability-of-salmonella-enterica-to-biofilm-formation-under-conditions-of-interspecies-interaction-with-representatives-of-the-intestinal-microflora/72525.html
MANAJEMEN PENGEMBANGAN KURIKULUM MUATAN LOKAL DI RA NU BANAT KUDUS
Authors: Fadlilah Siti
Published: November 2021

AbstractThis research aimed to describe the planning, organization, implementation, and evaluation of local content curriculum development at RA NU Banat Kudus. This research used qualitative approach and belongs to descriptive design. Data collection was done through observation, in depth interview, and documentation. The data was then analyzed by using triangulation technique. The research results showed that: 1) planning of local content curriculum development at RA NU Banat Kudus is done in the beginning of academic year and the material of local content is adjusted to the curriculum of Religion Ministry of Kudus. 2) Organizing of local content curriculum development classified by students’ age and taught classically in the classroom. 3) The implementation of development including the characteristic of local content curriculum (scope of teaching material, purpose, function, etc.) and implementation strategy. 4) Evaluation of local content curriculum development in learning process is done every day (daily achievement scale).Keywords: Curriculum Management, Curriculum Development, Local Content
KEPEMIMPINAN TRANFORMATIF KEPALA SEKOLAH DALAM MEWUJUDKAN PROFESIONALITAS MANAJEMEN MADRASAH (Studi Kasus MA Darul Ulum Kota Semarang)
Authors: Inderasari Elen
Published: November 2021

Era disrupsi dibutuhkan pemimpin yang berani bersikap, kritis, dan inovatif dalam menjawab tantangan zaman. Pemimpin era disrupsi harus memiliki karakter kepemimpinan yang kuat. Tujuan yang hendak dicapai dalam pembahasan Mengetahui kepemimpinan tranformatif kepala madrasah dalam mewujudkan profesionalitas guru di madrasah; dan manajemen optimalisasi dalam mewujudkan profesionalitas guru di madrasah melalui kepemimpinan transformatif di MA Darul Ulum Kota Semarang. Metode penulisan ini penelitian kualitatif pola pendekatan studi kasus. Data penelitian menggunakan data primer yang berasal dari hasil interview dan observasi serta data sekunder yang berasal dari dokumen. Sumber data dalam penelitian ini berwujud kata dan tindakan selebihnya adalah data tambahan seperti dokumen dan lain-lain. Gaya pemimpinan transformatif yang diterapkan di MA Darul Ulum Kota Semarang Kepemimpinan transformatif ini dalam konsep Ki Hajar Dewantara semboyannya Ing Ngarsa Sung Tuladha, Ing Madya Mangun Karsa, Tut Wuri Handayani. Managemen kepemimpinan kepala sekolah MA Darul Ulum Kota Semarang dalam mengoptimalkan profesionalitas guru meliputi perencanaan (planning), pengorganisasian (organizing), Koordinasi (coordinating), dan pengawasan (controlling). Perilaku kepemimpinan dalam upaya melaksanakan keterampilan memimpin memegang peranan yang sangat penting untuk untuk meningkatkan kualitas sekolah. Perilaku kepemimpinan yang positif sangat mendukung terhadap penerapan manajemen kepala sekolah akan lebih mencapai keberhasilan.