bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigating the Effect of the State, Stability, and Change in Deep Approaches to Learning From Kindergarten to Third Grade: A Multilevel Structural Equation Modeling Indicator-Specific Growth Model Approach
Authors: Wu Chung Chin
Published: March 2022

Adopting deep approaches to learning can have a profound impact on learning outcomes. The extent of change in the learning approach could be attributed to the effect of contextual factors (e.g., instruction). After a substantive review, it was found that research interested in investigating the longitudinal effect of deep approaches to learning on learning outcomes were rarely directly concerned with the longitudinal state and trend of the approach itself. Moreover, the limitations of past analytical methods (e.g., using a single indicator to represent deep approaches to learning at each point in time), has not been appropriately acknowledged. This study examined deep approaches to learning in students from kindergarten to third grade using a multilevel structural equation modeling indicator-specific growth model (MSEM-ISGM). This was used to highlight the methodological issues (e.g., consider four indicators separately at each wave) to investigate the effect of context and the states, stability, and changes in deep approaches to learning over this developmental period. The current study comprised of a large-scale sample of 21,490 kindergarteners in kindergarten. Results showed: (1) there was a contextual effect on the longitudinal changes of deep approaches to learning; (2) deep approaches to learning was high but showed considerable individual differences; (3) most indicators relating to deep approaches to learning declined (however, one increased), whereas the trend were relatively stable over time. Corresponding suggestions were proposed at the end of this article.
The Impact of Qigong and Tai Chi Exercise on Drug Addiction: A Systematic Review and Meta-Analysis
Authors: Cui Jiabao , Liu Fang , Liu Xuan , Li Ru , Chen Xiaorong , Zeng Hongfa
Published: March 2022

Background

Previous preliminary studies have found that qigong exercises produced significant effects in healthy people and in various clinical populations. The purpose of this study was to systematically review the effects of qigong and tai chi exercise on individuals with drug addiction.

Methods

A systematic search of seven English databases and three Chinese databases was conducted to identify randomized controlled trials (RCTs) and non-randomized comparative studies (NRS) assessing the effects of qigong and tai chi on drug addiction. Study quality was assessed using the Checklist for the Evaluation of Non-Pharmaceutical Trial Reports (CLEAR-NPT).

Results

Two RCTs and nine NRS studies were included in this study, including a total of 1072 patients with drug addiction (age range, 27–43 years). The results showed that qigong and tai chi exercise had a significant overall effect on depression (SMD = −0.353, 95%CI [−0.548, −0.159]), anxiety (SMD = −0.541, 95%CI [−0.818, −0.264]), quality of life (SMD = 0.673, 95%CI [0.438, 0.907]), and sleep quality (SMD = −0.373, 95%CI [−0.631, −0.116]). The subgroup analysis found that qigong outperformed tai chi on the improving depression, anxiety, and sleep quality.

Conclusion

Existing studies suggest that qigong and tai chi are effective at improving depression, anxiety, and quality of life in drug users; however, the evidence from rigorous randomized controlled group trials is lacking.
COVID-19: Analysis of Factors Affecting the Economy of Hunan Province Based on the Spatial Econometric Model
Authors: Pan Wen-Tsao , Zhonghuan Wu , Shiqi Chen , Siyi Xiao , Yanping Tang , Danying Liang
Published: March 2022

The COVID-19 pandemic has spread across the country negatively impacting on the economy. This paper uses the panel data of 14 prefecture-level cities from 2015 to 2020 in Hunan to determine the factors and effects of economic downturns based on the spatial econometric model. We calculate the Moran index, so-called the Moran's I, to analyse the impact of each factor on the economy. The results show that the spatial correlation of the cities around Chang-Zhu-Tan is high, and the economic growth of the entire province can be influenced by these cities. These cities should adopt strategies to improve the economy, such as reducing the tax revenues, improving the local financial revenues, and reducing the ineffective educational input. These results can also be helpful for policymakers, who will attempt to retransform the Hunan economy during the post-COVID era.
Gender Difference and Correlates of Physical Activity Among Urban Children and Adolescents in Ethiopia: A Cross-Sectional Study
Authors: Biadgilign Sibhatu , Gebremichael Bereket , Abera Admas , Moges Tsedey
Published: March 2022

Background

Studies indicate that children and adolescent populations in most countries show a low level of physical activity (PA) and an increasing prevalence of obesity. Addressing gender disparity in PA is the main element of public health programs. There is currently a paucity of studies, particularly, in developing countries that investigate gender differences and correlates of PA among children and adolescents.

Objective

The study is aimed to assess the gender difference and correlates of PA among children and adolescents in Ethiopia.

Methods

An observational population-based cross-sectional study was conducted in representative samples of children and adolescents in the capital city of Ethiopia, Addis Ababa. Multivariable logistic regression models with robust estimation of SEs were fitted to predict the odds ratios (ORs) and 95% CIs.

Results

A total of 632 children and adolescents-parent dyads were included in the study. More boys than girls (17.0 and 11.7%) were engaged in moderate intensity PA 3 days a week or more (p = 0.057). Age, mothers working in a private business, attending public schools, longer sleep duration, and being taught the benefits of PA were positively associated with meeting moderate-to-vigorous PA (MVPA) in both sexes combined and in a sub-sample of boys. Furthermore, an inverse association was found between overweight/obesity and MVPA in the overall children and girls as well. For moderate PA (MPA); the age of the children, maternal education and occupation, school type, overweight/obesity, and sleep duration on school nights were significant correlates among the studied children.

Conclusions

The present study provided evidence of several correlates identified associated with meeting MVPA and MPA in both sexes combined. Girls are less likely than boys to engage in PA. Therefore, there is a need to take into perspectives the provision of a comprehensive multifaceted health behavior modification and interventions, such as focused and regular physical education in schools.
Grouping Together to Fight Cancer: The Role of WeChat Groups on the Social Support and Self-Efficacy
Authors: Zhong Fangqi , Pengpeng Li , Qianru Zhuo
Published: March 2022

With the increasing number of cancer survivors, the question of how to coexist with cancer has become more and more pressing. This research uses a mutual help WeChat group organized by cancer patients as the research field to observe the daily interactions of cancer patients, so as to improve understanding of how social media technology can help cancer patients in the treatment and recovery process. The study found that the WeChat group is the main source of health knowledge for the participating cancer patients, and that when compared to traditional web-based patient mutual aid communities, the WeChat group is a more timely, popular, continuous, and accurate source of information. Patients in the group can listen and respond to each other's questions and worries, providing both an outlet for patients to vent their emotions and concerns and a source of recognition and encouragement. In addition, this study found that the WeChat mutual aid group improves patients' self-efficacy of disease on four levels: successful experience in curing patients, imitating patients' behavior, verbal persuasion, and emotional support.
Profiles of Social Capital and the Association With Depressive Symptoms Among Multicultural Adolescents in Korea: A Latent Profile Analysis
Authors: Kim Eunjoo , Song Min Kyung
Published: March 2022

Low social capital has been reported to be associated with depression in adolescents. In general, adolescents with immigrant backgrounds lack social capital. By employing a latent profile analysis (LPA) for the specification of social capital among multicultural adolescents, depression interventions can be explored through the framework of social capital. The purpose of this study was to identify distinct latent profiles of social capital and explore the depressive symptoms of multicultural adolescents in those profiles. Data of 1,244 adolescents from the Multicultural Adolescents Panel Survey (MAPS) were used, which was conducted for 3rd-year middle school students in 2016. An LPA was used to identify profiles by different social capital classes and Quade's non-parametric ANCOVA was used to confirm the differences in depressive symptoms between profiles. Latent profile analysis indicated four classes. Analysis revealed that there were differences in the level of depressive symptoms according to the social capital sub-profiles (F = 44.42, p < 0.001). Class 1 had the lowest level of depressive symptoms (13.91 ± 4.43) and Class 4 had the highest level (18.07 ± 5.25). The depressive symptoms scores of Classes 2 and 3 were 16.49 ± 5.27 and 16.74 ± 4.95, respectively. These findings provide insight into the interplay between social capital and depressive symptoms among multicultural adolescents. Support in consideration of differences in social capital is needed to reduce depression among multicultural adolescents.
Policy Effects of Ecological Red Lines on Industrial Upgrading and Health Promotion: Evidence From China Based on DID Model
Authors: Ye Penghao
Published: March 2022

The implementation of the Ecological Red Lines (ERL) policy in China is under the background that natural resources have been immoderately exploited for serving rapid economic growth in the last 40 years, where the ecosystem's degradation happened and people's health could be affected. As the secondary industry is the contribution source of rapid growth as well as the threat source that threatens the natural environment and public health, the delimitation of ERL can act as a legal restriction that forces the industries to control the emissions and to upgrade the industrial composition. This paper conducts an ex-post policy evaluation on the improvement effects of industrial structure and residents' health and through ERL's pilot scheme in four provinces of China. By using the difference-in-differences (DID) method, the estimation results show that: (1) The industrial upgrading effect exists but to a small extent, as the ERL policy has generally elevated the tertiary industry's output by only 0.033% and hardly shown any promotion effects on the ratio of the tertiary industry to secondary industry; (2) The residents' health has been significantly improved by 1.029% after ERL policy on the whole, and enhanced over time mostly; (3) The health promotion effects are similar among three out of the four pilot provinces, whereas the industrial upgrading effects performed large heterogeneities among the four. These empirical results may provide references for the wider extension of ERL policy with more practical execution solutions in developing economies.
Clonal Hematopoiesis Analyses in Clinical, Epidemiologic, and Genetic Aging Studies to Unravel Underlying Mechanisms of Age-Related Dysfunction in Humans
Authors: Walsh Kenneth , Raghavachari Nalini , Kerr Candace , Bick Alexander G. , Cummings Steven R. , Druley Todd , Dunbar Cynthia E. , Genovese Giulio , Goodell Margaret A. , Jaiswal Siddhartha , Maciejewski Jaroslaw , Natarajan Pradeep , Shindyapina Anastasia V. , Shuldiner Alan R. , Van Den Akker Erik B. , Vijg Jan
Published: March 2022

Aging is characterized by increased mortality, functional decline, and exponential increases in the incidence of diseases such as cancer, stroke, cardiovascular disease, neurological disease, respiratory disease, etc. Though the role of aging in these diseases is widely accepted and considered to be a common denominator, the underlying mechanisms are largely unknown. A significant age-related feature observed in many population cohorts is somatic mosaicism, the detectable accumulation of somatic mutations in multiple cell types and tissues, particularly those with high rates of cell turnover (e.g., skin, liver, and hematopoietic cells). Somatic mosaicism can lead to the development of cellular clones that expand with age in otherwise normal tissues. In the hematopoietic system, this phenomenon has generally been referred to as “clonal hematopoiesis of indeterminate potential” (CHIP) when it applies to a subset of clones in which mutations in driver genes of hematologic malignancies are found. Other mechanisms of clonal hematopoiesis, including large chromosomal alterations, can also give rise to clonal expansion in the absence of conventional CHIP driver gene mutations. Both types of clonal hematopoiesis (CH) have been observed in studies of animal models and humans in association with altered immune responses, increased mortality, and disease risk. Studies in murine models have found that some of these clonal events are involved in abnormal inflammatory and metabolic changes, altered DNA damage repair and epigenetic changes. Studies in long-lived individuals also show the accumulation of somatic mutations, yet at this advanced age, carriership of somatic mutations is no longer associated with an increased risk of mortality. While it remains to be elucidated what factors modify this genotype-phenotype association, i.e., compensatory germline genetics, cellular context of the mutations, protective effects to diseases at exceptional age, it points out that the exceptionally long-lived are key to understand the phenotypic consequences of CHIP mutations. Assessment of the clinical significance of somatic mutations occurring in blood cell types for age-related outcomes in human populations of varied life and health span, environmental exposures, and germline genetic risk factors will be valuable in the development of personalized strategies tailored to specific somatic mutations for healthy aging.
Dancing My Age: Emotions, Interactions, and Bodily Sensations
Authors: Heikkinen Satu , Wilinska Monika
Published: March 2022

While there is a growing body of research on the social aspects of older people's dance, studies focusing on emotions are rare. In this study, we use an interactionist sociological perspective to examine the role of emotions in older social dancers' experiences in Sweden. Through qualitative interviews with 29 active or previously active dancers, we found that their experiences of emotional energy and experiences of flow override concerns of age and aging. Age, however, did become significant as the age differences at dance events could bring forth feelings of alienation associated with feeling old. In addition, cultural and gendered norms of appropriate age differences between dancing partners produced shame and pride as well as feelings of being either old or young. Moreover, certain bodily experiences were interpreted in terms of age. Overall, the study contributes to the discussions of the complexity of subjective experiences of age by highlighting its emotional aspects through social partner dancing.
Environmental Assessment of Austrian Organic Dairy Farms With Closed Regional Production Cycles in a Less Favorable Production Area
Authors: Grassauer Florian , Herndl Markus , Iten Laura , Gaillard Gérard
Published: March 2022

Extensive dairy production in less favorable production areas has a long tradition in Austria. Nevertheless, dairy production also contributes considerable environmental impacts (EIs), e.g., greenhouse gas emissions, nutrient losses, and land use. Therefore, 20 organic dairy farms located in the Lungau region in Austria were assessed concerning their EIs via life cycle assessment (LCA). Cumulative exergy demand (CExD), normalized eutrophication potential (EP), aquatic ecotoxicity potential (AE), and global warming potential (GWP) were considered as impact categories to describe the farms' EIs. The farms were part of a pilot project aiming to produce high-quality dairy products and keep production cycles closed within the project region. Consequently, the purchase of key off-farm resources was only possible within the project region. We adapted existing life cycle inventories to account for those regional resource purchases. Subsequently, the EIs of the 20 farms were related to the functional units (FUs) of 1 kg energy-corrected milk (ECM) and 1 ha agricultural area for milk production and compared to a representative model dairy farm (MDF) that was created based on statistical data and average production values of organic Austrian dairy farms. Compared to the MDF, results show an ~58% lower EP per ha and 44% per kg ECM of the Lungau farms. Further, the CExD per ha was about 24% lower due to a lower use of resources caused by the lower production intensity of the Lungau farms. Regarding GWP, Lungau farms are favorable considering 1 ha as the FU, whereas the MDF seems advantageous if 1 kg ECM is used as the FU. However, caused by a high variation of purchased roughage and the lower production intensity, the Lungau farms cause higher AE, regardless of the FU. Overall, we identified three principal production parameters determining the environmental performance of milk production in a closed production cycle in a less favorable area, namely, (1) the stocking rate, (2) the fed concentrate, and (3) the purchased roughage. Using those inputs at moderate intensity, the extensively managed Lungau farms can competitively contribute to producing food, thus highlighting the importance of site-adapted agriculture.
Tryptamine 5-Hydroxylase Is Required for Suppression of Cell Death and Uncontrolled Defense Activation in Rice
Authors: Shen Wangxin , Feng Zhiming , Hu Keming , Cao Wenlei , Li Mengchen , Ju Ran , Zhang Yafang , Chen Zongxiang , Zuo Shimin
Published: March 2022

Lesion-mimic mutants are useful materials to dissect mechanisms controlling programmed cell death (PCD) and defense response in plants. Although dozens of lesion-mimic mutant genes have been identified in plants, the molecular mechanisms underlying PCD and defense response remain to be extensively elucidated. Here, we identified a rice lesion mimic mutant, named lesion mimic 42 (lm42), from an ethylmethylsulfone (EMS)-induced mutant population. The lm42 mutant displayed flame-red spots on the leaves and sheaths at the 3-leaf developmental stage and exhibited impaired photosynthetic capacity with decreased chlorophyll content and decomposed chloroplast thylakoids. The lesion development of lm42 was light- and temperature-dependent. We identified a single base mutation (T38A), changing a Leu to Gln, in the first exon of LOC_Os12g16720 (LM42), which encodes a tryptamine 5-hydroxylase, by map-based cloning. We carried out transgenic complementation to confirm that this mutation caused the lm42 phenotype. We further knocked out the LM42 gene by CRISPR/Cas9 to recreate the lm42 phenotype. LM42 is highly expressed in leaves, leaf sheaths and roots. Loss-of-function of LM42 activated expression of ROS-generating genes and inhibited expression of ROS-scavenging genes, leading to ROS accumulation and eventually cell death. Furthermore, its disruption induced expression of defense-response genes and enhanced host resistance to both fungal pathogen Magnaporthe oryzae and bacterial pathogen Xanthomonas oryzae pv. oryzae. Our transcriptomic data suggested that the way lm42 led to lesion-mimic was probably by affecting ribosome development. Overall, our results demonstrate that tryptamine 5-hydroxylase-coding gene LM42 is required for suppression of cell death and uncontrolled activation of defense responses in rice.
Current Opinion and Practice on Peritoneal Carcinomatosis Management: The North African Perspective
Authors: Souadka Amine , Essangri Hajar , Makni Amin , Abid Mourad , Ayadi Mouna , Ksantini Feriel , Kordjani Zakia , Ballah Yousri , Bouka Jemila , Benkabbou Amine , Majbar Mohammed Anass , El Khannoussi Basma , Mohsine Raouf , Boutayeb Saber , Hubner Martin
Published: March 2022

Background

The status of peritoneal surface malignancy (PSM) management in North Africa is undetermined. The aim of this study was to assess and compare current practice and knowledge regarding PSM and examine satisfaction with available treatment options and need for alternative therapies in North Africa.

Methods

This is a qualitative study involving specialists participating in PSM management in North Africa. The survey analyzed demographic characteristics and current knowledge and opinions regarding PSM management in different institutions. We also looked at goals and priorities, satisfaction with treatment modalities and heated intraperitoneal chemotherapy (HIPEC) usefulness according to specialty, country, years of experience, and activity sector.

Results

One-hundred and three participants responded to the survey (response rate of 57%), including oncologists and surgeons. 59.2% of respondents had more than 10 years experience and 45.6% treated 20–50 PSM cases annually. Participants satisfaction with PSM treatment modalities was mild for gastric cancer (3/10 [IQR 2–3]) and moderate for colorectal (5/10 [IQR 3–5]), ovarian (5/10 [IQR 3–5]), and pseudomyxoma peritonei (5/10 [IQR 3–5]) type of malignancies. Good quality of life and symptom relief were rated as main priorities for treatment and the need for new treatment modalities was rated 9/10 [IQR 8–9]. The perceived usefulness of systemic chemotherapy in first intention was described as high by 42.7 and 39.8% of respondents for PSM of colorectal and gastric origins, while HIPEC was described as highly useful for ovarian (49.5%) and PMP (73.8) malignancies.

Conclusions

The management of PSM in the North African region has distinct differences in knowledge, treatments availability and priorities. Disparities are also noted according to specialty, country, years of expertise, and activity sector. The creation of referral structures and PSM networks could be a step forward to standardized PSM management in the region.
Laparoscopy vs. Laparotomy for the Management of Abdominal Trauma: A Systematic Review and Meta-Analysis
Authors: Wang Jianjun , Cheng Liangwang , Liu Jing , Zhang Binyin , Wang Weijun , Zhu Wenxin , Guo Yan , Bao Chuanfei , Hu Yunli , Qi Shanxin , Wang Kai , Zhao Shuguang
Published: March 2022

Background

There is still no consensus regarding the role of laparoscopy in trauma cases. The purpose of this paper is to assess the value of diagnostic and therapeutic laparoscopy for patients with blunt or penetrating abdominal trauma by performing a systematic review and meta-analysis.

Methods

PubMed, Embase, and the Cochrane library were systemically searched for the randomized controlled trials (RCTs) and non-RCT comparative studies on effectiveness and safety of laparoscopy vs. laparotomy for the two authors independently performed the search, data extraction, and quality assessment.

Results

A total of 5,517 patients were enrolled in 23 eligible studies that were published in English. Meta-analysis results suggest that there is no significant difference in the incidence of missed injury and mortality between abdominal trauma patients receiving laparoscopy and those receiving laparotomy. Concerning postoperative complications, compared with patients in the open surgery group, those in the laparoscopy group are at a similar risk of intra-abdominal abscesses, thromboembolism, and ileus, while there is a decreased incidence of wound infection and pneumonia. Besides, patients in the laparoscopy group experience shorter hospitalization times and procedure times. For most outcomes, the sensitivity analysis yielded similar results to the primary analysis.

Conclusion

Laparoscopic surgery is a practical alternative to laparotomy for appropriate patients. The decision to perform laparoscopy should be based on the experience of the surgeon and the resources available.
Charcot Neuroarthropathy: Current Surgical Management and Update. A Systematic Review
Authors: Bajuri Mohd Yazid , Ong Shir Lee , Das Srijit , Mohamed Isa Naina
Published: March 2022

Background

Charcot neuroarthropathy of the ankle and the hindfoot is a complex clinical entity with a high risk of amputation. Charcot neuroarthropathy limb reconstruction has been proposed as a limb-salvaging procedure. However, there was a lack of information on the various available reconstruction methods, including the outcomes and complications. The present study aimed to evaluate the current literature and update on the trends regarding the surgical management of Charcot neuroarthropathy of the ankle and the hindfoot.

Methods

All data published from January 2010 to January 2020 that investigated the methods of fixation and their respective outcomes for the surgical reconstruction in Charcot neuroarthropathy were analyzed. The union rate, amputation rates, and complications associated with these techniques were taken for statistical analysis.

Results

A total of 16 studies fit the inclusion criteria of this study, with four Level-III studies and 12 Level-IV studies were included. Ten studies utilized internal fixation only; five used a combination of internal fixation and circular external fixator, whereby there are three comparative studies between internal and external fixations, and two studies applied combined technique of internal and external fixations (hybrid fixation). One study describes the usage of circular external fixation only.

Conclusions

The use of retrograde intramedullary nail as a treatment of choice in the reconstruction of Charcot neuroarthropathy ankle is recommended before an ulcer occurrence. Hydroxyapatite (HA)- coated screws are recommended for the locking mechanism to prevent migration in Charcot neuroarthropathy due to poor bony quality. Hybrid fixation is recommended for reconstruction in a condition of ulceration and more complex deformity as it provides a higher rate of limb salvage with less soft tissue irritation.
Special Considerations for Tympanoplasty Type I in the Oncological Pediatric Population: A Case-Control Study
Authors: Richard Celine , Baker Emily , Wood Joshua
Published: March 2022

Introduction

Although cutting-edges antineoplastic therapies increase survival in children with malignancies, the optimal surgical strategy to address associated comorbidities such as chronic tympanic membrane perforation is still poorly documented. The aim of this study is to evaluate the outcomes of type I tympanoplasty in pediatric cancer survivors who received chemo and/or radiotherapy to the skull and to identify potential associated risk factors.

Methods

This case-control study included medical records review of oncologic patients (age <21) treated at the same Academic medical oncologic center between March 2015 and July 2021 and referred for conductive hearing loss and chronic tympanic membrane perforation. Patients and middle ear status-related variables were analyzed, and outcomes were compared with matched peers without any history of malignancies.

Results

A total of seven pediatric cancer survivors and seven paired children without any history of malignancies were included in this report. The mean age at tympanoplasty type I surgery was 10.2 years (range = 4.3–19.9; median = 7.9 years) for the pediatric cancer survivors' group and 10.1 years (range = 5.5–19.2; median = 7.9 years) in the control group. Three pediatric cancer patients had received chemotherapy alone, one patient had radiotherapy to the skull base, and three patients had received chemoradiotherapy. On average, surgery was performed 3.9 years after chemo and/or radiotherapy termination, except for 1 patient for whom the tympanoplasty was performed during chemotherapy treatment. A retroauricular approach was used for one of the pediatric cancer patients, a transcanal approach was performed in one other and five patients benefited from an otoendoscopic approach. Tragal perichondrium with cartilage was used in most of the pediatric cancer survivor cases (four out seven cases) while xenograft (Biodesign) and Temporalis fascia without cartilage graft were used in five out of the seven control cases. Rate of tympanic membrane perforation recurrence was similar between groups (28.6%). Mean functional gain for air conduction Pure Tone Average (AC PTA) was 2.6 and 7.7 dB HL for the oncologic and control group, respectively. Mean postoperative air-bone gap (ABG) was 10.7 dB HL [median = 8.7; inter-quartile range (IQR) = 13.8] for the oncologic cohort and 10.1 dB HL (median = 10.7; IQR = 9.6) for the control group.

Discussion

Chemo- and chemoradiotherapy to the skull are associated with damages to the inner and middle ear structures with secondary eustachian tube dysfunction and chronic middle ear effusion. Although healing abilities and immunological defenses are compromised as part of the expected effects of antineoplastic therapies, type I tympanoplasty can be safe and effective in this population. While different approaches may be considered, otoendoscopy showed excellent results with less morbidity in this vulnerable population.
Primary and Secondary Genotoxicity of Nanoparticles: Establishing a Co-Culture Protocol for Assessing Micronucleus Using Flow Cytometry
Authors: Vallabani N. V. Srikanth , Karlsson Hanna L.
Published: March 2022

Genotoxicity is an important endpoint to assess for understanding the risks associated with nanoparticles (NPs). Most genotoxicity studies performed on NPs have focused on primary genotoxicity analyzed by comet- or micronuclei (MN) assay using microscopic scoring. Here, we established a protocol for a more efficient version of MN assessment using flow cytometry and, importantly, both primary and secondary (inflammation-driven) genotoxicity was assessed. Human bronchial epithelial cells (HBEC-3kt) were exposed to nickel oxide (NiO) NPs directly or indirectly. The indirect exposure was done to assess secondary genotoxicity, and in this case immune cells (THP-1 derived macrophages) were exposed on inserts and the HBEC were cultured in the lower compartment. The results in monocultures showed that no increased MN formation was observed in the HBEC cells but instead a clear MN induction was noted in THP-1 cells indicating higher sensitivity. No MN formation was either observed when the HBEC were indirectly exposed, but an increase in DNA strand breaks was detected using the comet assay. Taken together, the present study emphasizes the feasibility of assessing primary and secondary genotoxicity and, furthermore, shows a clear MN induction in THP-1 monoculture following NiO NPs exposure.
Ex Vivo Pharmacokinetics and Pharmacodynamics Modeling and Optimal Regimens Evaluation of Cefquinome Against Bovine Mastitis Caused by Staphylococcus aureus
Authors: Jiang Li-jie , Xiao Xia , Yan Ke-xu , Deng Tian , Wang Zhi-qiang
Published: March 2022

Cefquinome, the fourth-generation cephalosporin applied solely for veterinary medicine, is commonly used for bovine mastitis caused by Staphylococcus aureus. The present study aims to establish an optimal dose and provide a PK/PD Cutoff value (COPD) for cefquinome against S. aureus based on ex vivo pharmacokinetics and pharmacodynamics (PK/PD) integration. This study investigated the pharmacokinetics (PK) of cefquinome when administered as three consecutive intramammary (IMM) doses of cefquinome in three healthy dairy cows at 75 mg/gland. Drug concentration was determined by HPLC-MS/MS assay. The ex vivo pharmacodynamics (PD) of cefquinome were evaluated by using a milk sample from a PK experiment. The relationship between the AUC/ MIC of cefquinome and bacterial loading reduction was simulated using a Sigmoid Emax model. The cefquinome concentration in milk attained a maximum level of 1.55 ± 0.21 mg/mL at 1.8 h after the third administration. The mean value of the area under the concentration-time curve (AUC0−24) was 26.12 ± 2.42 mg·h/mL after the third administration. The elimination half-life was 10.6 h. For PD profile, the MICs of cefquinome in milk were 2–4 times higher than those in the broth. In vitro time-killing curve shows that initial bacterial concentration has a huge impact on antibacterial effect on three strains. The antibacterial effect was weakened with the initial bacterial concentration increasing from 106 to 108 CFU/mL. The AUC0−24h/MIC index correlated well with ex vivo efficacy both for the initial inoculum of 106 CFU/mL and 108 CFU/mL (R2 > 0.84). According to the inhibitory sigmoid Emax model analysis, the PK/PD surrogate (AUC0−24/MIC) values were 8,638, 1,397, and 3,851 for bactericidal effect (E = −3) with an initial inoculum of 106 CFU/mL, while the corresponding values were 12,266, 2,295, and 5,337, respectively, with the initial inoculum of 108 CFU/mL. The ex vivo PK/PD based population dose prediction indicated a target attainment rate (TAR) of 90% of 55 mg/gland/12 h. The COPD for cefquinome against S. aureus was 2 μg/mL under the recommended dose of 55 mg/gland/12 h. However, it should be validated in clinical practice in future investigations. These results contribute to the rational use of cefquinome for mastitis treatment in clinical veterinary medicine.
Prediction of Intrinsically Disordered Proteins Using Machine Learning Based on Low Complexity Methods
Authors: Zeng Xingming , Liu Haiyuan , He Hao
Published: March 2022

Prediction of intrinsic disordered proteins is a hot area in the field of bio-information. Due to the high cost of evaluating the disordered regions of protein sequences using experimental methods, we used a low-complexity prediction scheme. Sequence complexity is used in this scheme to calculate five features for each residue of the protein sequence, including the Shannon entropy, the Topo-logical entropy, the Permutation entropy and the weighted average values of two propensities. Particularly, this is the first time that permutation entropy has been applied to the field of protein sequencing. In addition, in the data preprocessing stage, an appropriately sized sliding window and a comprehensive oversampling scheme can be used to improve the prediction performance of our scheme, and two ensemble learning algorithms are also used to verify the prediction results before and after. The results show that adding permutation entropy improves the performance of the prediction algorithm, in which the MCC value can be improved from the original 0.465 to 0.526 in our scheme, proving its universality. Finally, we compare the simulation results of our scheme with those of some existing schemes to demonstrate its effectiveness.
A Seed-Guided Latent Dirichlet Allocation Approach to Predict the Personality of Online Users Using the PEN Model
Authors: Sagadevan Saravanan , Malim Nurul Hashimah Ahamed Hassain , Husin Mohd Heikal
Published: March 2022

There is a growing interest in topic modeling to decipher the valuable information embedded in natural texts. However, there are no studies training an unsupervised model to automatically categorize the social networks (SN) messages according to personality traits. Most of the existing literature relied on the Big 5 framework and psychological reports to recognize the personality of users. Furthermore, collecting datasets for other personality themes is an inherent problem that requires unprecedented time and human efforts, and it is bounded with privacy constraints. Alternatively, this study hypothesized that a small set of seed words is enough to decipher the psycholinguistics states encoded in texts, and the auxiliary knowledge could synergize the unsupervised model to categorize the messages according to human traits. Therefore, this study devised a dataless model called Seed-guided Latent Dirichlet Allocation (SLDA) to categorize the SN messages according to the PEN model that comprised Psychoticism, Extraversion, and Neuroticism traits. The intrinsic evaluations were conducted to determine the performance and disclose the nature of texts generated by SLDA, especially in the context of Psychoticism. The extrinsic evaluations were conducted using several machine learning classifiers to posit how well the topic model has identified latent semantic structure that persists over time in the training documents. The findings have shown that SLDA outperformed other models by attaining a coherence score up to 0.78, whereas the machine learning classifiers can achieve precision up to 0.993. We also will be shared the corpus generated by SLDA for further empirical studies.
Research on Agricultural Machinery Rental Optimization Based on the Dynamic Artificial Bee-Ant Colony Algorithm
Authors: Hou Jialin , Zhang Jingtao , Wu Wanying , Jin Tianguo , Zhou Kai
Published: March 2022

Agricultural machinery rental is a new service form that uses big data in agriculture to improve the utilization rate of agricultural machinery and promote the development of the agricultural economy. To realize agricultural machinery scheduling optimization in cloud services, a dynamic artificial bee-ant colony algorithm (DABAA) is proposed to solve the above problem. First, to improve the practicability of the mathematical model in agricultural production, a dynamic coefficient is proposed. Then the mutation operation is combined with the artificial bee colony (ABC) algorithm to improve the algorithm. Then, iterative threshold adjustment and optimal fusion point evaluation are used to combine the ABC algorithm with the ant colony optimization (ACO) algorithm, which not only improves the search precision but also improves the running speed. Finally, two groups of comparison experiments are carried out, and the results show that the DABAA can obviously improve the running speed and accuracy of cloud services in agricultural machinery rental.
AgroML: An Open-Source Repository to Forecast Reference Evapotranspiration in Different Geo-Climatic Conditions Using Machine Learning and Transformer-Based Models
Authors: Bellido-Jiménez Juan Antonio , Estévez Javier , Vanschoren Joaquin , García-Marín Amanda Penélope
Published: March 2022

Accurately forecasting reference evapotranspiration (ET0) values is crucial to improve crop irrigation scheduling, allowing anticipated planning decisions and optimized water resource management and agricultural production. In this work, a recent state-of-the-art architecture has been adapted and deployed for multivariate input time series forecasting (transformers) using past values of ET0 and temperature-based parameters (28 input configurations) to forecast daily ET0 up to a week (1 to 7 days). Additionally, it has been compared to standard machine learning models such as multilayer perceptron (MLP), random forest (RF), support vector machine (SVM), extreme learning machine (ELM), convolutional neural network (CNN), long short-term memory (LSTM), and two baselines (historical monthly mean value and a moving average of the previous seven days) in five locations with different geo-climatic characteristics in the Andalusian region, Southern Spain. In general, machine learning models significantly outperformed the baselines. Furthermore, the accuracy dramatically dropped when forecasting ET0 for any horizon longer than three days. SVM, ELM, and RF using configurations I, III, IV, and IX outperformed, on average, the rest of the configurations in most cases. The best NSE values ranged from 0.934 in Córdoba to 0.869 in Tabernas, using SVM. The best RMSE, on average, ranged from 0.704 mm/day for Málaga to 0.883 mm/day for Conil using RF. In terms of MBE, most models and cases performed very accurately, with a total average performance of 0.011 mm/day. We found a relationship in performance regarding the aridity index and the distance to the sea. The higher the aridity index at inland locations, the better results were obtained in forecasts. On the other hand, for coastal sites, the higher the aridity index, the higher the error. Due to the good performance and the availability as an open-source repository of these models, they can be used to accurately forecast ET0 in different geo-climatic conditions, helping to increase efficiency in tasks of great agronomic importance, especially in areas with low rainfall or where water resources are limiting for the development of crops.
Understanding microRNAs in the Context of Infection to Find New Treatments against Human Bacterial Pathogens
Authors: Mourenza Álvaro , Lorente-Torres Blanca , Durante Elena , Llano-Verdeja Jesús , Aparicio Jesús F. , Fernández-López Arsenio , Gil José A. , Mateos Luis M. , Letek Michal
Published: March 2022

The development of RNA-based anti-infectives has gained interest with the successful application of mRNA-based vaccines. Small RNAs are molecules of RNA of <200 nucleotides in length that may control the expression of specific genes. Small RNAs include small interference RNAs (siRNAs), Piwi-interacting RNAs (piRNAs), or microRNAs (miRNAs). Notably, the role of miRNAs on the post-transcriptional regulation of gene expression has been studied in detail in the context of cancer and many other genetic diseases. However, it is also becoming apparent that some human miRNAs possess important antimicrobial roles by silencing host genes essential for the progress of bacterial or viral infections. Therefore, their potential use as novel antimicrobial therapies has gained interest during the last decade. The challenges of the transport and delivery of miRNAs to target cells are important, but recent research with exosomes is overcoming the limitations in RNA-cellular uptake, avoiding their degradation. Therefore, in this review, we have summarised the latest developments in the exosomal delivery of miRNA-based therapies, which may soon be another complementary treatment to pathogen-targeted antibiotics that could help solve the problem caused by multidrug-resistant bacteria.
Empiric Treatment in HAP/VAP: “Don’t You Want to Take a Leap of Faith?”
Authors: Chaïbi Khalil , Péan de Ponfilly Gauthier , Dortet Laurent , Zahar Jean-Ralph , Pilmis Benoît
Published: March 2022

Ventilator-associated pneumonia is a frequent cause of ICU-acquired infections. These infections are associated with high morbidity and mortality. The increase in antibiotic resistance, particularly among Gram-negative bacilli, makes the choice of empiric antibiotic therapy complex for physicians. Multidrug-resistant organisms (MDROs) related infections are associated with a high risk of initial therapeutic inadequacy. It is, therefore, necessary to quickly identify the bacterial species involved and their susceptibility to antibiotics. New diagnostic tools have recently been commercialized to assist in the management of these infections. Moreover, the recent enrichment of the therapeutic arsenal effective on Gram-negative bacilli raises the question of their place in the therapeutic management of these infections. Most national and international guidelines recommend limiting their use to microbiologically documented infections. However, many clinical situations and, in particular, the knowledge of digestive or respiratory carriage by MDROs should lead to the discussion of the use of these new molecules, especially the new combinations with beta-lactamase inhibitors in empirical therapy. In this review, we present the current epidemiological data, particularly in terms of MDRO, as well as the clinical and microbiological elements that may be taken into account in the discussion of empirical antibiotic therapy for patients managed for ventilator-associated pneumonia.
Influence of Sub-Inhibitory Dosage of Cefotaxime on Multidrug Resistant Staphylococcus haemolyticus Isolated from Sick Neonatal Care Unit
Authors: Chakraborty Madhurima , Bardhan Taniya , Basu Manjari , Bhattacharjee Bornali
Published: March 2022

Staphylococcus haemolyticus has emerged to be a frequently encountered late-onset sepsis pathogen among newborn infants. Critical care of neonates involves substantial usage of antibiotics and these pathogens are often exposed to sub-optimal doses of antibiotics which can augment maintenance of selection determinants and a range of physiological effects, prime among them being biofilm formation. Therefore, in this study, the outcome of a sub-inhibitory dosage of a commonly prescribed third-generation antibiotic, cefotaxime (CTX), on multidrug resistant (MDR) S. haemolyticus, was investigated. A total of 19 CTX-resistant, MDR and 5 CTX-susceptible strains isolated from neonates were included. Biofilm-forming abilities of S. haemolyticus isolates in the presence of sub-optimal CTX (30 μg/mL) were determined by crystal violet assays and extracellular DNA (eDNA) quantitation. CTX was found to significantly enhance biofilm production among the non-susceptible isolates (p-valueWilcoxintest—0.000008) with an increase in eDNA levels (p-valueWilcoxintest—0.000004). Further, in the absence of antibiotic selection in vitro, populations of MDR isolates, JNM56C1 and JNM60C2 remained antibiotic non-susceptible after >500 generations of growth. These findings demonstrate that sub-optimal concentration of CTX induces biofilm formation and short-term non-exposure to antibiotics does not alter non-susceptibility among S. haemolyticus isolates under the tested conditions.
Performance of Novel Antimicrobial Protein Bg_9562 and In Silico Predictions on Its Properties with Reference to Its Antimicrobial Efficiency against Rhizoctonia solani
Authors: Karnati Pranathi , Gonuguntala Rekha , Barbadikar Kalyani M. , Mishra Divya , Jha Gopaljee , Prakasham Vellaisamy , Chilumula Priyanka , Shaik Hajira , Pesari Maruthi , Sundaram Raman Meenakshi , Chinnaswami Kannan
Published: March 2022

Bg_9562 is a potential broad-spectrum antifungal effector protein derived from the bacteria Burkholderia gladioli strain NGJ1 and is effective against Rhizoctonia solani, the causal agent of sheath blight in rice. In the present study, in vitro antifungal assays showed that Bg_9562 was efficient at 35 °C and 45 °C and ineffective either at high acidic pH (3.0) or alkaline pH (9.5) conditions. Compatibility studies between the native bioagents Trichoderma asperellum TAIK1 and Bacillus subtilis BIK3 indicated that Bg_9562 was compatible with the bioagents. A field study using foliar spray of the Bg_9562 protein indicated the need of formulating the protein before its application. In silico analysis predicted that Bg_9562 possess 111 amino acid residues (46 hydrophobic residues, 12 positive and 8 negative residues) with the high aliphatic index of 89.92, attributing to its thermostability with a half-life of 30 h. Bg_9562 (C491H813N137O166S5) possessed a protein binding potential of 1.27 kcal/mol with a better possibility of interacting and perturbing the membrane, the main target for antimicrobial proteins. The secondary structure revealed the predominance of random coils in its structure, and the best 3D model of Bg_9562 was predicted using an ab initio method with Robetta and AlphaFold 2. The predicted binding ligands were nucleic acids and zinc with confidence scores of 0.07 and 0.05, respectively. The N-terminal region (1–14 residues) and C-terminal region (101 to 111) of Bg_9562 residues were predicted to be disordered regions. Stability and binding properties of the protein from the above studies would help to encapsulate Bg_9562 using a suitable carrier to maintain efficiency and improve delivery against Rhizoctonia solani in the most challenging rice ecosphere.