bluuebunny/crossref_metadata_2025 huggingface dataset:
Pan-Genome-Wide Identification and Transcriptome-Wide Analysis of ZIP Genes in Cucumber
Authors: Wang Zimo , Yin Mengmeng , Han Jing , Wang Xuehua , Chang Jingshu , Ren Zhonghai , Wang Lina
Published: January 2024

The ZRT/IRT-like proteins (ZIPs) play critical roles in the absorption, transport, and intracellular balance of metal ions essential for various physiological processes in plants. However, little is known about the pan-genomic characteristics and properties of ZIP genes in cucumber (Cucumis sativus L.). In this study, we identified 10 CsZIP genes from the pan-genome of 13 C. sativus accessions. Among them, only CsZIP10 showed no variation in protein sequence length. We analyzed the gene structure, conserved domains, promoter cis-elements, and phylogenetic relationships of these 10 CsZIP genes derived from “9930”. Based on phylogenetic analysis, the CsZIP genes were classified into three branches. Amino acid sequence comparison revealed the presence of conserved histidine residues in the ZIP proteins. Analysis of promoter cis-elements showed that most promoters contained elements responsive to plant hormones. Expression profiling in different tissues showed that most CsZIP genes were expressed at relatively low levels in C. sativus leaves, stems, and tendrils, and CsZIP7 and CsZIP10 were specifically expressed in roots, indicating their potential involvement in the absorption and transport of metal ions. Transcriptomic data indicated that these 10 ZIP genes displayed responses to both downy mildew and powdery mildew, and CsZIP1 was significantly downregulated after both salt and heat treatments. In conclusion, this study deepens our understanding of the ZIP gene family and enhances our knowledge of the biological functions of CsZIP genes in C. sativus.
Autonecrotic Tomato (Solanum lycopersicum L.) Line as a Potential Model for Applications in Proximal Sensing of Biotic and Abiotic Stress
Authors: Santangelo Enrico , Giudice Angelo Del , Figorilli Simone , Violino Simona , Costa Corrado , Bascietto Marco , Bergonzoli Simone , Beni Claudio
Published: January 2024

The autonecrotic tomato line V20368 (working code IGSV) spontaneously develops necrotic lesions with acropetal progression in response to an increase in temperature and light irradiation. The process is associated with the interaction between tomato and Cladosporium fulvum, the fungal agent of leaf mold. The contemporary presence of an in-house allele encoding the Rcr3lyc protein and the resistance gene Cf-2pim (from Solanum pimpinellifolium) causes auto-necrosis on the leaves even in the absence of the pathogen (hybrid necrosis). The aim of the work was (i) to examine the potential value of the necrotic genotype as a model system for setting up theoretical guidance for monitoring the phytosanitary status of tomato plants and (ii) to develop a predictive model for the early detection of pathogens (or other stresses) in the tomato or other species. Eighteen IGSV tomato individuals at the 4–5th true-leaf stage were grown in three rows (six plants per row) considered to be replicates. The healthy control was the F1 hybrid Elisir (Olter). A second mutant line (SA410) deriving from a cross between the necrotic mutant and a mutant line of the lutescent (l) gene was used during foliar analysis via microspectrometry. The leaves of the mutants and normal plants were monitored through a portable VIS/NIR spectrometer SCIOTM (Consumer Physics, Tel Aviv, Israel) covering a spectral range between 740 and 1070 nm. Two months after the transplant, the acropetal progression of the autonecrosis showed three symptomatic areas (basal, median, apical) on each IGSV plant: necrotic, partially damaged, and green, respectively. Significantly lower chlorophyll content was found in the basal and median areas of IGSV compared with the control (Elisir). A supervised classification/modelling method (SIMCA) was used. Applying the SIMCA model to the dataset of 162 tomato samples led to the identification of the boundary between the healthy and damaged samples (translational critical distance). Two 10 nm wavelength ranges centred at 865 nm and 1055 nm exhibited a stronger link between symptomatology and spectral reflectance. Studies on specific highly informative mutants of the type described may allow for the development of predictive models for the early detection of pathogens (or other stresses) via proximal sensing.
Benefiting from Complexity: Exploring Enhanced Biological Control Effectiveness via the Simultaneous Use of Various Methods for Combating Pest Pressure in Agriculture
Authors: Curk Miha , Trdan Stanislav
Published: January 2024

Biological control, a well-established plant protection method, has garnered substantial attention in recent decades. Various approaches, including biological control agents (BCA), catch crops, biofumigation, sticky traps, and pheromones, have been extensively explored. While the effectiveness of these methods varies depending on specific circumstances, their collective significance has grown amid mounting pressures to curtail or eliminate conventional synthetic plant protection products. Previous review articles have highlighted the benefits of using two or more BCAs simultaneously, yet limited information exists regarding the concurrent use of diverse biological control methods. This comprehensive review incorporates a thorough literature search to assess the benefit of concurrently employing two or more of these methods, followed by a discussion on perspectives of holistic management and mimicking complex natural systems, shedding light on the vast potential and need for further research in this domain.
Phosphorus HotSpots in Crop Plants Production on the Farm—Mitigating Critical Factors
Authors: Grzebisz Witold , Niewiadomska Alicja , Potarzycki Jarosław , Andrzejewska Agnieszka
Published: January 2024

Phosphorus resources, both in phosphate rocks and in the soil, are limited. However, effective food production is not possible without the use of P fertilizers. Recognizing and eliminating or at least ameliorating factors (hot spots) that interfere with the uptake and use of phosphorus (P) by crop plants is of key importance for effective use of both P and nitrogen (N) on the farm. Plants have developed many adaptation mechanisms to their environment, i.e., soil low in available phosphorus. The most important ones include the secretion of organic compounds into the rhizosphere and the association of plant roots with microorganisms. A classic example is mycorrhiza. These mechanisms can be used by the farmer to sequentially select plants in the crop rotation. The uptake of inorganic P (Pi) by plants from the soil is reduced by environmental (temperature and water) and soil factors (low content of available phosphorus, soil acidity, soil compaction). These factors are responsible for the growth and size of the root system. Mitigating these negative effects improves the efficiency of phosphorus uptake from the soil. The second group of critical factors, limiting both root growth and availability of phosphorus, can be effectively controlled using simple measures (for example, lime). Knowing this, the farmer must first control the level of soil fertility in the plant’s effective rooting zone and not only in the topsoil. Secondly, the farmer must multiply the productivity of applied mineral fertilizers used through targeted recycling: crop rotation, crop residues, and manure.
Effects of Dietary Fiber Level and Forage Particle Size on Growth, Nutrient Digestion, Ruminal Fermentation, and Behavior of Weaned Holstein Calves under Heat Stress
Authors: Izadbakhsh Mohammad-Hossein , Hashemzadeh Farzad , Alikhani Masoud , Ghorbani Gholam-Reza , Khorvash Mohammad , Heidari Mostafa , Ghaffari Morteza Hosseini , Ahmadi Farhad
Published: January 2024

This experiment was designed to investigate the effects of feeding diets with different fiber content and forage particle size on the performance, health, nutrient digestion, rumen fermentation, and behavioral and sorting activity of Holstein dairy calves kept under elevated environmental temperature. Sixty weaned Holstein female calves (age = 96.7 ± 7.62 days old; body weight = 82.4 ± 10.4 kg) were randomly assigned to one of 4 treatments arranged in a 2-by-2 factorial design in a 70-day experiment. Dietary forage content (moderate, 22.5%; or high, 40.0% on DM basis) and alfalfa hay particle size (short, 4.39 mm; or long, 7.22 mm as geometric mean) were the experimental factors, resulting in the following combinations: (1) high-fiber (HF) diets with forage-to-concentrate ratio of 40:60 and long particle-sized alfalfa hay (LPS; HF-LPS); (2) HF diets with short particle-sized alfalfa hay (SPS; HF-SPS); (3) moderate-fiber (MF) diets with forage-to-concentrate ratio of 22.5:77.5 with LPS (MF-LPS); and (4) MF diets with SPS (MF-SPS). The temperature–humidity index averaged 73.0 ± 1.86, indicating that weaned calves experienced a moderate extent of heat stress. Fiber level and AH particle size interacted and affected dry matter intake, with the greatest intake (4.83 kg/d) observed in MF-SPS-fed calves. Final body weight was greater in calves receiving MF vs. HF diets (164 vs. 152 kg; p < 0.01). Respiration rate decreased when SPS vs. LPS AH was included in HF but not MF diet. Lower rectal temperature was recorded in calves fed MF vs. HF diet. Digestibility of dry matter and crude protein was greater in calves fed MF than HF diets, resulting in lower ruminal pH (6.12 vs. 6.30; p = 0.03). Fiber digestibility was greater in calves fed SPS compared with those fed LPS alfalfa hay. Feeding HF compared with MF diet increased acetate but lowered propionate molar proportions. The inclusion of SPS vs. LPS alfalfa hay decreased lying time in HF diet (920 vs. 861 min; p < 0.01). Calves fed MF vs. HF diets spent less time eating but more time lying, which is likely indicative of better animal comfort. Dietary fiber level and forage particle size interacted and affected sorting against 19 mm particles, the extent of which was greater in HF-SPS diet. Overall, dietary fiber level had a stronger effect than forage particle size on the performance of weaned calves exposed to a moderate degree of heat stress as feeding MF vs. HF diet resulted in greater feed intake, final body weight, structural growth measures, nutrient digestion, as well as longer lying behavior. The inclusion of SPS alfalfa hay in MF diets increased feed consumption.
Effects of Supplementing Milk Replacer with Sodium Butyrate on Dairy Calves
Authors: Belli Anna Luiza , Coelho Sandra G. , Campolina Joana P. , Neves Luiz F. M. , Neto Hilton C. Diniz , Silva Camila S. , Machado Fernanda S. , Pereira Luiz Gustavo R. , Tomich Thierry R. , Carvalho Wanessa A. , Costa Suely de Fátima , M. Campos Mariana
Published: January 2024

Diarrhea and respiratory diseases pose significant challenges in the rearing of pre-weaned calves, motivating the investigation of tools to improve gastrointestinal tract development, health, and overall performance in young calves. Consequently, the primary objective of this study was to assess the effectiveness of an additive incorporated into milk replacer to promote the development and health of the animals. Forty-six dairy calves were randomly assigned into two treatments: control (CON, n = 23; with 15 females and 8 males), and sodium butyrate (SB, n = 23; with 15 females and 8 males). The calves in the SB treatment group were supplemented with 4 g/d of unprotected sodium butyrate (Adimix, Adisseo, China), added to the milk replacer from 4 to 60 days of age. Water and starter were fed ad libitum. The study evaluated several parameters, including feed intake, nutrient digestibility, ruminal pH, ammonia and volatile fatty acids, blood metabolites (glucose, insulin-like growth factor type 1, urea, β–hydroxybutyrate), hemogram, health scores, performance, and feed efficiency. Bull calves were euthanized at 60 days of age for organ comparison, while heifer calves were assessed for carryover effects up to 90 days of age. Data were analyzed independently using linear mixed models using the nlme package in R, and the Artools package for non-parametric categorical outcomes. Although the feed intake and performance variables exhibited differences within weeks, no divergence was observed between treatment groups. Notably, a positive treatment-by-week interaction was identified for starter feed intake (p = 0.02) and total dry matter intake (p = 0.04) during pre-weaning for CON animals. Ruminal parameters, blood metabolites, and hemogram values such as glucose, urea, insulin-like growth factor type 1, mean corpuscular value, lymphocytes, and neutrophils displayed differences within weeks during the pre-weaning stage, but similar results within groups. No differences between supplemented and non-supplemented calves were found across nutrient digestibility, organ development, and histology. Regarding health scores, differences were noted within weeks for fecal and respiratory scores during the pre-weaning stage, and only the respiratory score during the post-weaning stage. Consequently, butyrate supplementation did not elicit improvements or negative effects in the body development or health status of dairy calves.
A System for Monitoring Animals Based on Behavioral Information and Internal State Information
Authors: Shibanoki Taro , Yamazaki Yuugo , Tonooka Hideyuki
Published: January 2024

Managing the risk of injury or illness is an important consideration when keeping pets. This risk can be minimized if pets are monitored on a regular basis, but this can be difficult and time-consuming. However, because only the external behavior of the animal can be observed and the internal condition cannot be assessed, the animal’s state can easily be misjudged. Additionally, although some systems use heartbeat measurement to determine a state of tension, or use rest to assess the internal state, because an increase in heart rate can also occur as a result of exercise, it is desirable to use this measurement in combination with behavioral information. In the current study, we proposed a monitoring system for animals using video image analysis. The proposed system first extracts features related to behavioral information and the animal’s internal state via mask R-CNN using video images taken from the top of the cage. These features are used to detect typical daily activities and anomalous activities. This method produces an alert when the hamster behaves in an unusual way. In our experiment, the daily behavior of a hamster was measured and analyzed using the proposed system. The results showed that the features of the hamster’s behavior were successfully detected. When loud sounds were presented from outside the cage, the system was able to discriminate between the behavioral and internal changes of the hamster. In future research, we plan to improve the accuracy of the measurement of small movements and develop a more accurate system.
Partial Correspondence between Host Plant-Related Differentiation and Symbiotic Bacterial Community in a Polyphagous Insect
Authors: Cheng Zhentao , Liu Qian , Huang Xiaolei
Published: January 2024

Host plants play a vital role in insect population differentiation, while symbiotic associations between bacteria and insects are ubiquitous in nature. However, existing studies have given limited attention to the connection between host-related differentiation and symbiotic bacterial communities in phytophagous insects. In this study, we collected 58 samples of Aphis odinae from different host plants in southern China and constructed phylogenetic trees to investigate their differentiation in relation to host plants. We also selected aphid samples from the five most preferred host plants and analyzed their symbiotic bacterial composition using Illumina sequencing of the V3–V4 hypervariable region of the 16S rRNA gene. The phylogeny and symbiotic bacterial community structure of A. odinae populations on different host plants showed that samples from Triadica sebifera (Euphorbiaceae) had a consistent presence of Wolbachia as the predominant secondary symbiont and suggested the possibility of undergoing differentiation. Conversely, although differentiation was observed in samples from Rhus chinensis (Anacardiaceae), no consistent presence of predominant secondary symbionts was found. Additionally, the samples from Heptapleurum heptaphyllum (Araliaceae) consistently carried Serratia, but no host differentiation was evident. In summary, this study reveals a partial correspondence between symbiotic bacterial communities and host-related differentiation in A. odinae. The findings contribute to our understanding of the microevolutionary influencing the macroevolutionary relationships between bacterial symbionts and phytophagous insects. The identification of specific symbionts associated with host-related differentiation provides valuable insights into the intricate dynamics of insect-bacteria interactions.
Oxidative Post-translational Protein Modifications upon Ischemia/Reperfusion Injury
Authors: Binek Aleksandra , Castans Celia , Jorge Inmaculada , Bagwan Navratan , Rodríguez José Manuel , Fernández-Jiménez Rodrigo , Galán-Arriola Carlos , Oliver Eduardo , Gómez Mónica , Clemente-Moragón Agustín , Ibanez Borja , Camafeita Emilio , Vázquez Jesús
Published: January 2024

While reperfusion, or restoration of coronary blood flow in acute myocardial infarction, is a requisite for myocardial salvage, it can paradoxically induce a specific damage known as ischemia/reperfusion (I/R) injury. Our understanding of the precise pathophysiological molecular alterations leading to I/R remains limited. In this study, we conducted a comprehensive and unbiased time-course analysis of post-translational modifications (PTMs) in the post-reperfused myocardium of two different animal models (pig and mouse) and evaluated the effect of two different cardioprotective therapies (ischemic preconditioning and neutrophil depletion). In pigs, a first wave of irreversible oxidative damage was observed at the earliest reperfusion time (20 min), impacting proteins essential for cardiac contraction. A second wave, characterized by irreversible oxidation on different residues and reversible Cys oxidation, occurred at late stages (6–12 h), affecting mitochondrial, sarcomere, and inflammation-related proteins. Ischemic preconditioning mitigated the I/R damage caused by the late oxidative wave. In the mouse model, the two-phase pattern of oxidative damage was replicated, and neutrophil depletion mitigated the late wave of I/R-related damage by preventing both Cys reversible oxidation and irreversible oxidation. Altogether, these data identify protein PTMs occurring late after reperfusion as an actionable therapeutic target to reduce the impact of I/R injury.
Oxidative Balance Score and New-Onset Type 2 Diabetes Mellitus in Korean Adults without Non-Alcoholic Fatty Liver Disease: Korean Genome and Epidemiology Study-Health Examinees (KoGES-HEXA) Cohort
Authors: Moon Mid-Eum , Jung Dong Hyuk , Heo Seok-Jae , Park Byoungjin , Lee Yong Jae
Published: January 2024

The oxidative balance score (OBS) is a novel composite of pro- and anti-oxidative markers for assessing the risk of cardiometabolic diseases and non-alcoholic fatty liver disease (NAFLD). However, it has not yet been established whether the OBS is related to type 2 diabetes mellitus (T2DM), especially in a population without NALFD. Therefore, we aimed to investigate the longitudinal effect of the OBS on T2DM in a large cohort of Korean adults without NALFD. Data were assessed from 9798 participants without NALFD from the Korean Genome and Epidemiology Study-Health Examinees (KoGES-HEXA) cohort. The participants were divided into three groups according to OBS tertiles, identified as T1–T3. We prospectively assessed the hazard ratios (HRs) with 95% confidence intervals (CIs) for new-onset T2DM using multivariable Cox proportional hazard regression models over 6 years following the baseline survey. During the mean 3.5 years of follow-up, 145 individuals (1.48%; 56 men and 89 women) developed T2DM. The HRs of T2DM for the OBS tertiles were 0.79 (95% CI, 0.53–1.18) and 0.60 (95% CI, 0.39–0.93) in the T2 and T3 groups after adjusting for metabolic parameters in subjects without NALFD, respectively; however, the T2 group did not show statistical significance toward a decrease in incident T2DM. A low OBS may be a useful predictive marker in new-onset T2DM for middle-aged and older subjects without NALFD. This implies that the OBS could be an additional valuable tool for assessing the incidence of T2DM among individuals without NAFLD.
Study on the Residual Strength of Nonlinear Fatigue-Damaged Pipeline Structures
Authors: Wei Wenlan , Zhang Rui , Zhang Yuwen , Cheng Jiarui , Cao Yinping , Fang Fengying
Published: January 2024

The heat alternation and bending during the service process of the pipeline structure are the key issues affecting its residual strength. The nonuniform plastic deformation of its cross-section causes nonlinear damage to the structure, which makes it difficult to calculate the residual strength of the overall structure. This study proposed a novel variable-strength material damage model with damage accumulation, which achieved the coupling analysis of structural damage and material fatigue residual strength, making the structural strength of nonlinear damage cross-section computable. Based on this, a theoretical ultimate load model for nonlinear damage cross-section and a finite element analysis model for dynamic material strength with damage accumulation were established. The analysis was conducted on the repeated banding damage to coiled tubing. The results showed that the residual yield strength of nonlinear damaged coiled tubing showed a trend of first rapid decrease and then slow decrease with damage accumulation, and the tensile displacement showed an increasing trend with damage accumulation. The ultimate internal pressure strength of nonlinear damaged structures showed a similar downward trend to the yield strength. The nonlinear damage coupling analysis model proposed in this study has significant practical engineering application value.
The Nutritional Value Adequacy and Microbiological Quality of Canned Foods for Puppies and Adult Dogs
Authors: Kazimierska Katarzyna , Biel Wioletta , Witkowicz Robert , Kochel-Karakulska Jolanta
Published: January 2024

The pet food industry’s growth, driven by increased demand for premium options, emphasizes the popularity of canned dog foods due to their palatability and convenience. This study evaluates the nutritional and microbiological quality of canned dog food for puppies and adult dogs, with and without grains, immediately after opening and following 24 h of incubation simulating home storage conditions. The grain-free products exhibited higher protein and energy levels, while the grain-included products contained more ether extract, crude ash, and nitrogen-free extract. Age-specific differences revealed higher ether extract, crude ash, crude fiber, and energy in growing dog foods and more nitrogen-free extract in adult dog foods. Discrepancies between labeled nutrient values and laboratory results indicated overstatements for ether extract and underestimations for protein and crude ash. A microbiological analysis showed that the canned dog foods were generally safe when opened, but after 24 h, 85% exhibited bacterial presence, emphasizing the need for rigorous evaluation. A statistical analysis did not confirm associations between grain presence or age group and microbiological contamination. This study underscores the importance of accurate labeling for nutritional value and emphasizes the need for microbiological safety evaluations, especially in regions lacking specific regulatory standards, to ensure pet food safety and nutritional adequacy.
Least-Cost-Path and Closest Facility Analysis for Generating District Heating Networks on a Communal Level
Authors: Strzalka Aneta , Malicki Jacek , Blachowski Jan
Published: January 2024

Due to an urgent need to reduce the fast-progressing climate changes, a rapid, standardized and replicable solution for the infrastructure restructuring of the heat demand coverage of buildings at the district and city levels must be developed. As more and more communities in Europe, especially in Germany, are obligated to have plans for coverage of the heat demand, city planners and energy agencies seek a tool that will help them to design the first drafts of heating network routes, which can supply whole districts and cities with renewable energy. ArcGIS Pro Tools like Least-Cost-Path Analysis (LCPA) and Closest Facility (CF) allow us to find the shortest and “cheapest” way between the heat source and heat consumers in the analyzed areas. Starting from the community level, through the district, county, voivodeship and whole-country levels, the replication of the methods for generating district heating (DH) network infrastructure can be achieved. The proposed LCPA- and CF-based methods help to design the most suitable and efficient DH networks in the analyzed areas. As only a few open-source data inputs, like street networks and building footprints, are needed, the methods can be implemented in all communities in the country of Poland. In this paper, one example of one community in Wroclaw county, called Siechnice, and its surroundings is presented.
A New Indicator for Measuring Efficiency in Urban Freight Transportation: Defining and Implementing the OEEM (Overall Equipment Effectiveness for Mobility)
Authors: Les Adrián , Morella Paula , Lambán María Pilar , Royo Jesús , Sánchez Juan Carlos
Published: January 2024

Urban freight transportation is the activity that has the greatest impact on urban areas in terms of sustainability and livability, and it is, therefore, necessary to reduce its impact. Currently, there is a lack of methodologies to validate the methods proposed by companies to reduce their impacts. The proposed methodology presents the implementation of a KPI (Key Performance Indicator) based on the triple bottom line approach: economic, social and environmental, since a company with good results on the “triple bottom line” will experience an increase in its economic profitability and its environmental commitment while reducing the impacts that generate negative perceptions of it. This KPI is the OEEM (Overall Equipment Effectiveness for Mobility), a redesign of the well-known OEE (Overall Equipment Effectiveness), but adapted to the needs of urban freight transportation since this indicator provides a quick overview of the efficiency or performance of the activity according to five components: quality of deliveries, vehicle utilization, availability of the vehicle–driver tandem and efficiency (result of traffic and efficiency of delivery stops). The methodology developed will be implemented in a case study where the KPI will be calculated on the basis of real-time data and visualized on a control panel; thanks to this KPI, the company will be able to validate whether the measures taken have a positive or negative impact.
Deep Learning and YOLOv8 Utilized in an Accurate Face Mask Detection System
Authors: Dewi Christine , Manongga Danny , Hendry  , Mailoa Evangs , Hartomo Kristoko Dwi
Published: January 2024

Face mask detection is a technological application that employs computer vision methodologies to ascertain the presence or absence of a face mask on an individual depicted in an image or video. This technology gained significant attention and adoption during the COVID-19 pandemic, as wearing face masks became an important measure to prevent the spread of the virus. Face mask detection helps to enforce mask-wearing guidelines, which can significantly reduce the spread of respiratory illnesses, including COVID-19. Wearing masks in densely populated areas provides individuals with protection and hinders the spread of airborne particles that transmit viruses. The application of deep learning models in object recognition has shown significant progress, leading to promising outcomes in the identification and localization of objects within images. The primary aim of this study is to annotate and classify face mask entities depicted in authentic images. To mitigate the spread of COVID-19 within public settings, individuals can employ the use of face masks created from materials specifically designed for medical purposes. This study utilizes YOLOv8, a state-of-the-art object detection algorithm, to accurately detect and identify face masks. To analyze this study, we conducted an experiment in which we combined the Face Mask Dataset (FMD) and the Medical Mask Dataset (MMD) into a single dataset. The detection performance of an earlier research study using the FMD and MMD was improved by the suggested model to a “Good” level of 99.1%, up from 98.6%. Our study demonstrates that the model scheme we have provided is a reliable method for detecting faces that are obscured by medical masks. Additionally, after the completion of the study, a comparative analysis was conducted to examine the findings in conjunction with those of related research. The proposed detector demonstrated superior performance compared to previous research in terms of both accuracy and precision.
Peptide-Guided Nanoparticle Drug Delivery for Cardiomyocytes
Authors: Li Dong , Taylor Austin , Shi Haiwang , Zhou Fang , Li Pengsheng , Joshi Jyotsna , Zhu Wuqiang , Wang Shu
Published: January 2024

Background: Nanoparticles (NPs) have been extensively utilized as a drug delivery system to control the release of therapeutic agents to treat cardiac injuries. However, despite the advantages of utilizing NP-based drug delivery for treating heart diseases, the current delivery system lacks specificity in targeting the cardiac tissue, thus limiting its application. Methods: We created three linear peptides, each consisting of 16–24 amino acids. These peptides were conjugated on the surface of NPs, resulting in the formation of cardiac targeting peptide (CTP)-NPs (designated as CTP-NP1, CTP-NP2, and CTP-NP3). To assess their effectiveness, we compared the binding efficiency of these three CTP-NPs to human and mouse cardiomyocytes. Additionally, we determined their distribution 24 h after injecting the CTP-NPs intravenously into adult C57BL/6J mice. Results: When compared to control NPs without CTP (Con-NPs), all three CTP-NPs exhibited significantly increased binding affinity to both human and mouse cardiomyocytes in vitro and enhanced retention in mouse hearts in vivo. A thorough assessment of the heart sections demonstrated that the binding specificity of CTP-NP3 to cardiomyocytes in vivo was significantly greater than that of Con-NPs. None of the three CTP-NPs were proven to cause cardiomyocyte apoptosis. Conclusions: Biocompatible and safe CTP-NP3 can target the heart via binding to cardiomyocytes. This approach of targeting specific molecules-coated NPs may help in delivering therapeutic compounds to cardiomyocytes for the treatment of heart diseases with high efficacy and low toxicity to other tissues.
Ladostigil Reduces the Adenoside Triphosphate/Lipopolysaccharide-Induced Secretion of Pro-Inflammatory Cytokines from Microglia and Modulate-Immune Regulators, TNFAIP3, and EGR1
Authors: Reichert Fanny , Zohar Keren , Lezmi Elyad , Eliyahu Tsiona , Rotshenker Shlomo , Linial Michal , Weinstock Marta
Published: January 2024

Treatment of aging rats for 6 months with ladostigil (1 mg/kg/day) prevented a decline in recognition and spatial memory and suppressed the overexpression of gene-encoding pro-inflammatory cytokines, TNFα, IL1β, and IL6 in the brain and microglial cultures. Primary cultures of mouse microglia stimulated by lipopolysaccharides (LPS, 0.75 µg/mL) and benzoyl ATPs (BzATP) were used to determine the concentration of ladostigil that reduces the secretion of these cytokine proteins. Ladostigil (1 × 10−11 M), a concentration compatible with the blood of aging rats in, prevented memory decline and reduced secretion of IL1β and IL6 by ≈50%. RNA sequencing analysis showed that BzATP/LPS upregulated 25 genes, including early-growth response protein 1, (Egr1) which increased in the brain of subjects with neurodegenerative diseases. Ladostigil significantly decreased Egr1 gene expression and levels of the protein in the nucleus and increased TNF alpha-induced protein 3 (TNFaIP3), which suppresses cytokine release, in the microglial cytoplasm. Restoration of the aberrant signaling of these proteins in ATP/LPS-activated microglia in vivo might explain the prevention by ladostigil of the morphological and inflammatory changes in the brain of aging rats.
Systemic Characterization of the Gut Microbiota Profile after Single Mild Ischemic Stroke and Recurrent Stroke in Mice
Authors: Yang Decao , Sun Panxi , Chen Yong , Jin Haojie , Xu Baohui , Ma Qingbian , Xue Lixiang , Wang Yan
Published: January 2024

It has been estimated that one in four stroke patients may have recurrent stroke within five years after they experienced the first stroke. Furthermore, clinical studies have shown that recurrent stroke negatively affects patient outcomes; the risk of disability and the death rate increase with each recurrent stroke. Therefore, it is urgent to find effective methods to prevent recurrent stroke. The gut microbiota has been proven to play an essential role after ischemic stroke, while sudden ischemia disrupts microbial dysbiosis, and the metabolites secreted by the microbiota also reshape the gut microenvironment. In the present study, we established a recurrent ischemic mouse model. Using this experimental model, we compared the survival rate and ischemic infarction between single MCAO and recurrent MCAO, showing that, when two surgeries were performed, the mouse survival rate dramatically decreased, while the infarction size increased. Fecal samples were collected on day 1, day 3 and day 7 after the first MCAO and day 9 (2 days after the second MCAO) for 16S sequencing, which provided a relatively comprehensive picture of the microbiota changes. By further analyzing the potential metabolic pathways, our data also highlighted several important pathways that were significantly altered after the first and recurrent stroke. In the present study, using an experimental mouse model, we showed that acute ischemic stroke, especially recurrent ischemia, significantly decreased the diversity of the gut microbiota.
Aggregation-Resistant, Turn-On-Off Fluorometric Sensing of Glutathione and Nickel (II) Using Vancomycin-Conjugated Gold Nanoparticles
Authors: Tiwari Atul Kumar , Gupta Munesh Kumar , Yadav Hari Prakash , Narayan Roger J. , Pandey Prem C.
Published: January 2024

Glutathione (GSH) and nickel (II) cation have an indispensable role in various physiological processes, including preventing the oxidative damage of cells and acting as a cofactor for lipid metabolic enzymes. An imbalance in the physiological level of these species may cause serious health complications. Therefore, sensitive and selective fluorescent probes for the detection of GSH and nickel (II) are of great interest for clinical as well as environmental monitoring. Herein, vancomycin-conjugated gold nanoparticles (PEI-AuNP@Van) were prepared and employed for the detection of GSH and nickel (II) based on a turn-on-off mechanism. The as-synthesized PEI-AuNP@Van was ~7.5 nm in size; it exhibited a spherical shape with face-centered cubic lattice symmetry. As compared to vancomycin unconjugated gold nanoparticles, GSH led to the turn-on state of PEI-AuNP@Van, while Ni2+ acted as a fluorescence quencher (turn-off) without the aggregation of nanoparticles. These phenomena strongly justify the active role of vancomycin conjugation for the detection of GSH and Ni2+. The turn-on-off kinetics was linearly proportional over the concentration range between 0.05–0.8 µM and 0.05–6.4 μM. The detection limits were 205.9 and 90.5 nM for GSH and Ni2+, respectively; these results are excellent in comparison to previous reports. This study demonstrates the active role of vancomycin conjugation for sensing of GSH and Ni2+ along with PEI-AuNP@Van as a promising nanoprobe.
Stacking Ensemble-Based Machine Learning Model for Predicting Deterioration Components of Steel W-Section Beams
Authors: Khoshkroodi A. , Parvini Sani H. , Aajami M.
Published: January 2024

The collapse evaluation of the structural systems under seismic loading necessitates identifying and quantifying deterioration components (DCs). In the case of steel w-section beams (SWSB), three distinct types of DCs have been derived. These deterioration components for steel beams comprise the following: pre-capping plastic rotation (θp), post-capping plastic rotation (θpc), and cumulative rotation capacity (Λ). The primary objective of this research is to employ a machine learning (ML) model for accurate determination of these deterioration components. The stacking model is a powerful combination of meta-learners, which is used for better learning and performance of base learners. The base learners consist of AdaBoost, Random Forest (RF), and XGBoost. Among various machine learning algorithms, the stacking model exhibited superior functioning. The evaluation metrics of the stacking model were as follows: R2 = 0.9 and RMSE = 0.003 for θp, R2 = 0.97 and RMSE = 0.012 for θpc, and R2 = 0.98 and RMSE = 0.09 for Λ. The significance of input variables, specifically the web-depth-over-web-thickness ratio (h/tw) and the flange width-to-thickness ratio (bf/2tf), in determining the deterioration components was assessed using the Shapley Additive Explanations model. These parameters emerged as the most crucial factors in the evaluation.
Success Factors and Barriers for Facility Management in Keeping Nearly-Zero-Energy Non-Residential Buildings Energy-Efficient over Time
Authors: Lane Anna-Lena , Cehlin Mathias , Thollander Patrik
Published: January 2024

Energy efficiency is a cornerstone of climate change mitigation. For buildings, facility management is an essential part of achieving efficient energy use while keeping tenants satisfied. This interview study explores success factors and barriers for facility management in maintaining energy efficiency over time in four approximately 10-year-old non-residential premises built as so-called nearly zero-energy buildings (nZEB) in Sweden. The study highlights the importance of functional digital tools, benchmarks, and building professionals’ involvement in ensuring energy efficiency. It also emphasizes the need for involvement communication and strategies to engage facility management in energy efficiency efforts. The study suggests that in-house and public policies can play a crucial role in sustaining high ambitions for energy efficiency. Access to professional support that is self-evident to use is identified as a critical success factor. Additionally, the research presents an analytic model that can be used in future studies to assess facility management organizations’ potential for maintaining energy performance in buildings over time.
Mono- and Bimetallic Nanoparticles in Catalysis
Authors: Redina Elena A. , Kirichenko Olga A.
Published: January 2024

The catalytic applications of supported mono- and bimetallic nanoparticles are wide on the laboratory, pilot plant and industrial scale [...]
SWI/SNF Complex in Vascular Smooth Muscle Cells and Its Implications in Cardiovascular Pathologies
Authors: Liu Hongyu , Zhao Yang , Zhao Guizhen , Deng Yongjie , Chen Y. Eugene , Zhang Jifeng
Published: January 2024

Mature vascular smooth muscle cells (VSMC) exhibit a remarkable degree of plasticity, a characteristic that has intrigued cardiovascular researchers for decades. Recently, it has become increasingly evident that the chromatin remodeler SWItch/Sucrose Non-Fermentable (SWI/SNF) complex plays a pivotal role in orchestrating chromatin conformation, which is critical for gene regulation. In this review, we provide a summary of research related to the involvement of the SWI/SNF complexes in VSMC and cardiovascular diseases (CVD), integrating these discoveries into the current landscape of epigenetic and transcriptional regulation in VSMC. These novel discoveries shed light on our understanding of VSMC biology and pave the way for developing innovative therapeutic strategies in CVD treatment.
Effect of Microstructure on Corrosion Behavior of Cold Sprayed Aluminum Alloy 5083
Authors: Kim Munsu , Perez-Andrade Lorena , Brewer Luke N. , Kubacki Gregory W.
Published: January 2024

This paper investigates the effect of the microstructure on the corrosion behavior of cold sprayed (CS) AA5083 compared to its wrought counterpart. It has been shown that the microstructure of CS aluminum alloys, such as AA2024, AA6061, and AA7075, affects their corrosion behavior; however, investigations of the corrosion behavior of CS AA5083 with a direct comparison to wrought AA5083 have been limited. The microstructure and corrosion behavior of CS AA5083 were examined by scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), electron backscattered diffraction (EBSD), electrochemical and immersion tests, and ASTM G67. The CS process resulted in microstructural changes, such as the size and spatial distribution of intermetallic particles, grain size, and misorientation. The refined grain size and intermetallic particles along prior particle boundaries stimulated the initiation and propagation of localized corrosion. Electrochemical tests presented enhanced anodic kinetics with high pitting susceptibility, giving rise to extensive localized corrosion in CS AA5083. The ASTM G67 test demonstrated significantly higher mass loss for CS AA5083 compared to its wrought counterpart due to preferential attack within prior particle boundary regions in the CS microstructure. Possible mechanisms of intergranular corrosion (IGC) propagation at prior particle boundary regions have been discussed.
Effect of Oxidant Concentration on Properties of Ferrite Films by Spin-Spray Deposition
Authors: Liu Hai , Liao Jihong , Huang Gang , Jiang Xiaona , Yu Zhong , Lan Zhongwen , Sun Ke
Published: January 2024

In response to the demands for high frequency, miniaturization, and high integration in electronic devices, such as inductors and DC-DC convertors, nickel–zinc ferrite thin films exhibit significant application value and development potential. For regulating the magnetic properties and microstructure of spin-sprayed polycrystalline ferrite materials, a comprehensive understanding of the impact of oxidant concentration on film reaction is essential. This study finds that as the concentration of the NaNO2 oxidant increases, the grain size of the nickel–zinc ferrite thin film samples progressively enlarges. Due to the preferential occupation of iron ions at the B sites, the saturation magnetization correspondingly increases. However, when the oxidant concentration becomes excessive, the preferential (222) orientation growth of the film is disrupted, leading to the agglomeration and uneven growth of grains, transitioning from triangular plate-like to spherical in shape. This increase in grain size alters the magnetization mechanism of the thin film, predominantly favoring domain wall movement. Upon analyzing the microstructure and magnetic characteristics, it becomes evident that the concentration of oxidant is a key determinant in the spin-spray deposition process.
Influence of Interlayers on Adhesion Strength of TiN Film on Mg Alloy
Authors: Liu Huaiyuan , Li Jialin , Ma Donglin , Jiang Xin , Xie Dong , Leng Yongxiang
Published: January 2024

The wide application of Mg alloys has been restricted because of their poor corrosion and wear resistance. Titanium nitride ceramic films prepared via magnetron sputtering can improve the corrosion and wear resistance of Mg alloys. However, residual stress produced at the interface between the film and the Mg alloy substrate causes the TiN film to spall off and reduces its service life. One potential approach to mitigating residual stress involves enhancing the adhesive strength between the film and the substrate, thereby potentially extending the service life of the film. To increase the adhesion strength between the TiN film and the Mg alloy substrate, a Ti or Al interlayer was deposited on the Mg alloy by magnetron sputtering. Subsequently, the adhesion strengths of TiN/Ti and TiN/Al were determined under a single high shear force by scratch tests and were determined under multiple low shear forces by friction and wear tests. The results of scratch tests show that TiN with the Ti interlayer on the Mg alloy substrate has superior adhesion strength under a single high shear force. And the results of friction and wear tests show that both the TiN/Al and TiN/Ti films provided protection to the Mg alloy substrate against friction and wear, and TiN with the Ti interlayer on the Mg alloy substrate has superior adhesion strength under multiple low shear forces. This work can provide guidance for the selection of interlayers between Mg alloy substrates and hard ceramic films.
Inclusions and Spectral Characterization of Demantoid from Baluchistan, Pakistan
Authors: Zhang Jian-Yi , Li Geng , Tian Yu , Schmitz Fabian
Published: January 2024

Demantoid is the green variety of andradite [Ca3Fe2(SiO4)3], an exceptionally rare and precious gemstone worldwide. In recent years, a small amount of gem-quality demantoid has been found in Pakistan. This research focuses on nine demantoids sourced from Muslim Bagh, Baluchistan, Pakistan, presenting a comprehensive analysis of the spectral characteristics and inclusions of Pakistani demantoid using classical gemological methods, energy dispersive X-ray fluorescence (EDXRF) chemical analyses, Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, and ultraviolet and visible (UV-vis) spectroscopy. The results show that the content of Cr and V in most samples is lower than the detection line of EDXRF, with only one sample containing a Cr2O3 content of 0.032%. The extremely low Cr content sets Pakistani demantoid apart from demantoid of the serpentinite type found in other regions. Notably, the UV-vis spectrum reveals characteristic absorption at 443 nm due to Fe3+, while a further contribution from Cr3+ would be highly likely, and weak absorption at 550 nm caused by Fe3+. This suggests that iron (Fe) is the primary chromogenic element of Pakistani demantoid, but the role of Cr3+ cannot be ignored. The FTIR spectrum of Pakistani demantoid displays the absorption peaks associated with [SiO4]4− groups at 937 cm−1, 848 cm−1, and 817 cm−1, while the absorption peaks resulting from trivalent cations appear at 481 cm−1 and 442 cm−1, which are the characteristic FTIR spectra of demantoid. Raman spectroscopy further reveals absorption peaks are displayed near 994 cm−1, 843 cm−1, 818 cm−1, associated with (Si–O)Str vibrations (Si–O stretching vibration), and absorption peaks are displayed near 350 cm−1 and 310 cm−1, related to the rotation of SiO4–R(SiO4)4−, and the peaks near 514 cm−1 and 494 cm−1 are related to (Si–O)bend vibrations (Si–O bending vibration). Additionally, related absorption peaks near 168 cm−1 are attributed to the translation of SiO4–T(SiO4)4−, and absorption peaks near 234 cm−1 are associated with the translation of X2+–T(X2+) (X2+ represents divalent ions). The common dark opaque inclusions found in Pakistani demantoid consist of a combination of magnetite and hematite. Additionally, some samples of Pakistani demantoid display inclusions of calcite. This unique combination of inclusions differentiates Pakistani demantoid from demantoids sourced from other regions. It signifies that Pakistani demantoid has a distinctive geological origin resulting from the interplay of serpentinization and skarnization processes. This geological formation distinguishes it from demantoids solely hosted in serpentinite or skarn environments in other origins. The identification of these characteristics holds significant importance for accurately determining the origin of Pakistani demantoid.