bluuebunny/crossref_metadata_2025 huggingface dataset:
Editorial for the Special Issue: Advances in the Biology of Phototrophic Bacteria
Authors: Imhoff Johannes F.
Published: October 2021

Phototrophic bacteria represent a very ancient phylogenetic and highly diverse metabolic type of bacteria that diverged early into several major phylogenetic lineages with quite different properties [...]
Fungi Dominated the Incorporation of 13C-CO2 into Microbial Biomass in Tomato Rhizosphere Soil under Different CO2 Concentrations
Authors: Wang Hehua , Wang Juan , Ge Chaorong , Yao Huaiying
Published: October 2021

An elevated CO2 (eCO2) fumigation experiment was carried out to study the influence of various CO2 concentrations on microorganisms involved in the incorporation of root-derived C in greenhouse soil systems. In this study, 400 and 800 µmol·mol−1 CO2 fumigation treatments were conducted during tomato planting. Phospholipid fatty acid (PLFA) profiling based on the stable isotope probing (SIP) technique was applied to trace active microorganisms. The absolute total abundance of 13C-PLFAs was much higher under eCO2 treatment. Most of the 13C-CO2 was incorporated into the 13C-PLFAs 18:2ω6,9 (fungi), 16:0 (general PLFA), 18:1ω9c (Gram-negative bacteria, G−) and i17:0 (Gram-positive bacteria, G+) via rhizodeposition from tomato under ambient CO2 (aCO2) and eCO2 treatments, suggesting similar responses of active microorganisms to different CO2 treatments. However, the fungi (characterized by the 13C-PLFA 18:2ω6,9) played a much more dominant role in the incorporation of root-derived C under eCO2. Actinomycetes, marked by the 13C-PLFA 10-Me-18:0, occurred only on labeling day 15 under the eCO2 treatment, indicating that the actinomycetes fed on both soil organic carbon and fresh rhizodeposition. It was indicated that eCO2 significantly affected microbial biomass and microbial community structures involved in the incorporation of 13C-CO2 via tomato root secretions, as supported by Adonis analysis and the Mantel test.
Qualitative and Quantitative Characteristics of Soil Microbiome of Barents Sea Coast, Kola Peninsula
Authors: Korneykova Maria , Nikitin Dmitry , Myazin Vladimir
Published: October 2021

The soil microbiome of the Barents Sea coast of the Kola Peninsula is here characterized for the first time. The content of copies of ribosomal genes of archaea, bacteria, and fungi was determined by real-time PCR. Reserves and structure of biomass of soil microorganisms such as total biomass of fungi and prokaryotes, length and diameter of mycelium of fungi and actinomycetes, proportion of mycelium in biomass, number of spores and prokaryotic cells, proportion of small and large fungal propagules, and morphology of mycobiota spores were determined. The largest number of ribosomal gene copies was found for bacteria (from 6.47 × 109 to 3.02 × 1011 per g soil). The number of copies of ribosomal genes of fungi and archaea varied within 107–109 copies of genes/g soil. The biomass of microorganisms (prokaryotes and fungi in total) varied from 0.023 to 0.840 mg/g soil. The share of mycobiota in the microbial biomass ranged from 90% to 97%. The number of prokaryotes was not large and varied from 1.87 × 108 to 1.40 × 109 cells/g of soil, while the biomass of fungi was very significant and varied from 0.021 to 0.715 mg/g of soil. The length of actinomycete mycelium was small—from 0.77 to 88.18 m/g of soil, as was the length of fungal hyphae—an order of magnitude higher (up to 504.22 m/g of soil). The proportion of fungal mycelium, an active component of fungal biomass, varied from 25% to 89%. Most (from 65% to 100%) of mycobiota propagules were represented by specimens of small sizes, 2–3 microns. Thus, it is shown that, despite the extreme position on the mainland land of Fennoscandia, local soils had a significant number of microorganisms, on which the productivity of ecosystems largely depends.
Microbiomics in Collusion with the Nervous System in Carcinogenesis: Diagnosis, Pathogenesis and Treatment
Authors: Hull Rodney , Lolas Georgios , Makrogkikas Stylianos , Jensen Lasse D. , Syrigos Konstantinos N. , Evangelou George , Padayachy Llewellyn , Egbor Cyril , Mehrotra Ravi , Makhafola Tshepiso Jan , Oyomno Meryl , Dlamini Zodwa
Published: October 2021

The influence of the naturally occurring population of microbes on various human diseases has been a topic of much recent interest. Not surprisingly, continuously growing attention is devoted to the existence of a gut brain axis, where the microbiota present in the gut can affect the nervous system through the release of metabolites, stimulation of the immune system, changing the permeability of the blood–brain barrier or activating the vagus nerves. Many of the methods that stimulate the nervous system can also lead to the development of cancer by manipulating pathways associated with the hallmarks of cancer. Moreover, neurogenesis or the creation of new nervous tissue, is associated with the development and progression of cancer in a similar manner as the blood and lymphatic systems. Finally, microbes can secrete neurotransmitters, which can stimulate cancer growth and development. In this review we discuss the latest evidence that support the importance of microbiota and peripheral nerves in cancer development and dissemination.
Composted Municipal Green Waste Infused with Biocontrol Agents to Control Plant Parasitic Nematodes—A Review
Authors: Tóthné Bogdányi Franciska , Boziné Pullai Krisztina , Doshi Pratik , Erdős Eszter , Gilián Lilla Diána , Lajos Károly , Leonetti Paola , Nagy Péter István , Pantaleo Vitantonio , Petrikovszki Renáta , Sera Bozena , Seres Anikó , Simon Barbara , Tóth Ferenc
Published: October 2021

The last few years have witnessed the emergence of alternative measures to control plant parasitic nematodes (PPNs). We briefly reviewed the potential of compost and the direct or indirect roles of soil-dwelling organisms against PPNs. We compiled and assessed the most intensively researched factors of suppressivity. Municipal green waste (MGW) was identified and profiled. We found that compost, with or without beneficial microorganisms as biocontrol agents (BCAs) against PPNs, were shown to have mechanisms for the control of plant parasitic nematodes. Compost supports a diverse microbiome, introduces and enhances populations of antagonistic microorganisms, releases nematicidal compounds, increases the tolerance and resistance of plants, and encourages the establishment of a “soil environment” that is unsuitable for PPNs. Our compilation of recent papers reveals that while the scope of research on compost and BCAs is extensive, the role of MGW-based compost (MGWC) in the control of PPNs has been given less attention. We conclude that the most environmentally friendly and long-term, sustainable form of PPN control is to encourage and enhance the soil microbiome. MGW is a valuable resource material produced in significant amounts worldwide. More studies are suggested on the use of MGWC, because it has a considerable potential to create and maintain soil suppressivity against PPNs. To expand knowledge, future research directions shall include trials investigating MGWC, inoculated with BCAs.
Comparative Evaluation of Four Potent Neospora caninum Diagnostic Antigens Using Immunochromatographic Assay for Detection of Specific Antibody in Cattle
Authors: Fereig Ragab M. , Abdelbaky Hanan H. , Nishikawa Yoshifumi
Published: October 2021

Neospora caninum is an intracellular protozoan parasite responsible for numerous abortion outbreaks and neonatal abnormalities in cattle. Rapid and accurate diagnosis is critical for N. caninum control owing to the lack of vaccine or drug-based control strategies. Herein, we evaluated the performance of four frequently used antigens in the diagnosis of N. caninum infection using immunochromatographic tests (ICTs) as a rapid, affordable, and field applicable tool. These antigens included recombinant proteins of N. caninum surface antigen 1 (NcSAG1), dense granule proteins 7 (NcGRA7) and 6 (NcGRA6), in addition to native Neospora lysate antigen (NLA). Our study revealed the utility of all antigen-based ICTs for detection of specific antibodies to N. caninum. However, the NcSAG1-based ICT was the best for detection of all control N. caninum-infected mouse or cattle sera, while NcGRA7 and NcGRA6-based ICTs exhibited specific ability to detect samples from acute and sub-acute infection in mice and cattle, respectively. Analyses of the NcSAG1-based ICT against enzyme-linked immunosorbent assays (ELISAs) of the same antigen revealed its efficiency in detection of field cattle samples as observed in high sensitivity (84.2%), specificity (93.5%), agreement (90%), and kappa value (0.78). The current knowledge provides an efficient platform for N. caninum control through on-site diagnosis of infected cattle.
Correction: Khan et al. Anticancer Function and ROS-Mediated Multi-Targeting Anticancer Mechanisms of Copper (II) 2-hydroxy-1-naphthaldehyde Complexes. Molecules 2019, 24, 2544
Authors: Khan Muhammad Hamid , Cai Meiling , Deng Jungang , Yu Ping , Liang Hong , Yang Feng
Published: October 2021

Due to the previous incorrect characterization of compound C1, the authors wish to make the following corrections to this paper [...]
Study on Nuclear Magnetic Resonance Logging T2 Spectrum Shape Correction of Sandstone Reservoirs in Oil-Based Mud Wells
Authors: Sun Jianmeng , Cai Jun , Feng Ping , Sun Fujing , Li Jun , Lu Jing , Yan Weichao
Published: October 2021

The oil-based mud filtrate will invade the formation under the overbalanced pressure during drilling operations. As a result, alterations will occur to the nuclear magnetic resonance (NMR) response characteristics of the original formation, causing the relaxation time of the NMR T2 spectrum of the free fluid part to move towards a slower relaxation time. Consequently, the subsequent interpretation and petrophysical evaluation will be heavily impacted. Therefore, the actual measured T2 spectrum needs to be corrected for invasion. For this reason, considering the low-porosity and low-permeability of sandstone gas formations in the East China Sea as the research object, a new method to correct the incorrect shape of the NMR logging T2 spectrum was proposed in three main steps. First, the differences in the morphology of the NMR logging T2 spectrum between oil-based mud wells and water-based mud wells in adjacent wells were analyzed based on the NMR relaxation mechanism. Second, rocks were divided into four categories according to the pore structure, and the NMR logging T2 spectrum was extracted using the multidimensional matrix method to establish the T2 spectrum of water-based mud wells and oil-based mud wells. Finally, the correctness of the method was verified by two T2 spectrum correction examples of oil-based mud wells in the study area. The results show that the corrected NMR T2 spectrum eliminates the influence of oil-based mud filtrate and improves the accuracy of NMR logging for calculating permeability.
Cudraxanthone D Ameliorates Psoriasis-like Skin Inflammation in an Imiquimod-Induced Mouse Model via Inhibiting the Inflammatory Signaling Pathways
Authors: Kim Namkyung , Lee Soyoung , Kang Jinjoo , Choi Young-Ae , Jang Yong Hyun , Jeong Gil-Saeng , Kim Sang-Hyun
Published: October 2021

Psoriasis is a chronic inflammatory skin disease accompanied by excessive keratinocyte proliferation. Corticosteroids, vitamin D3 analogs, and calcineurin inhibitors, which are used to treat psoriasis, have diverse adverse effects, whereas natural products are popular due to their high efficiency and relatively low toxicity. The roots of the Cudrania tricuspidata (C. tricuspidata) are known to have diverse pharmacological effects, among which the anti-inflammatory effect is reported as a potential therapeutic agent in skin cells. Nevertheless, its effectiveness against skin diseases, especially psoriasis, is not fully elucidated. Here, we investigated the effect of cudraxanthone D (CD), extracted from the roots the C. tricuspidata Bureau, on psoriasis using an imiquimod (IMQ)-induced mouse model and the tumor necrosis factor (TNF)-α/interferon (IFN)-γ-activated keratinocytes. IMQ was topically applied to the back skin of C57BL/6 mice for seven consecutive days, and the mice were orally administered with CD. This resulted in reduced psoriatic characteristics, such as the skin thickness and Psoriasis Area Severity Index score, and the infiltration of neutrophils in IMQ-induced skin. CD inhibited the serum levels of TNF-α, immunoglobulin G2a, and myeloperoxidase, and the expression of Th1/Th17 cells in splenocytes. In TNF-α/IFN-γ-activated keratinocytes, CD reduced the expressions of CCL17, IL-1β, IL-6, and IL-8 by inhibiting the phosphorylation of STAT1 and the nuclear translocation of NF-kB. Taken together, these results suggest that CD could be a potential drug candidate for the treatment of psoriasis.
Multimode Robust Lasing in a Dye-Doped Polymer Layer Embedded in a Wedge-Shaped Cholesteric
Authors: Sarukhanyan Tatevik M. , Gharagulyan Hermine , Rafayelyan Mushegh S. , Golik Sergey S. , Gevorgyan Ashot H. , Alaverdyan Roman B.
Published: October 2021

Cholesteric liquid crystals (CLCs) with induced defects are one of the most prominent materials to realize compact, low-threshold and tunable coherent light sources. In this context, the investigation of optical properties of induced defect modes in such CLCs is of great interest. In particular, many studies have been devoted to the spectral control of the defect modes depending on their thickness, optical properties, distribution along the CLC, etc. In this paper, we investigate the lasing possibilities of a dye-doped polymer layer embedded in a wedge-shaped CLC. We show that multimode laser generation is possible due to the observed multiple defect modes in the PBG that enlarges the application range of the system. Furthermore, our simulations based on a Berreman 4 × 4 matrix approach for a wide range of CLC thickness show both periodic and continuous generation of defect modes along particular spectral lines inside the PBG. Such a robust spectral behaviour of induced defect modes is unique, and, to our knowledge, is not observed in similar CLC-based structures.
Glycyrrhizic Acid Inhibits SARS-CoV-2 Infection by Blocking Spike Protein-Mediated Cell Attachment
Authors: Li Jingjing , Xu Dongge , Wang Lingling , Zhang Mengyu , Zhang Guohai , Li Erguang , He Susu
Published: October 2021

Glycyrrhizic acid (GA), also known as glycyrrhizin, is a triterpene glycoside isolated from plants of Glycyrrhiza species (licorice). GA possesses a wide range of pharmacological and antiviral activities against enveloped viruses including severe acute respiratory syndrome (SARS) virus. Since the S protein (S) mediates SARS coronavirus 2 (SARS-CoV-2) cell attachment and cell entry, we assayed the GA effect on SARS-CoV-2 infection using an S protein-pseudotyped lentivirus (Lenti-S). GA treatment dose-dependently blocked Lenti-S infection. We showed that incubation of Lenti-S virus, but not the host cells with GA prior to the infection, reduced Lenti-S infection, indicating that GA targeted the virus for infection. Surface plasmon resonance measurement showed that GA interacted with a recombinant S protein and blocked S protein binding to host cells. Autodocking analysis revealed that the S protein has several GA-binding pockets including one at the interaction interface to the receptor angiotensin-converting enzyme 2 (ACE2) and another at the inner side of the receptor-binding domain (RBD) which might impact the close-to-open conformation change of the S protein required for ACE2 interaction. In addition to identifying GA antiviral activity against SARS-CoV-2, the study linked GA antiviral activity to its effect on virus cell binding.
Aurones and Flavonols from Coreopsis lanceolata L. Flowers and Their Anti-Oxidant, Pro-Inflammatory Inhibition Effects, and Recovery Effects on Alloxan-Induced Pancreatic Islets in Zebrafish
Authors: Kim Hyoung-Geun , Nam Youn Hee , Jung Young Sung , Oh Seon Min , Nguyen Trong Nguyen , Lee Min-Ho , Kim Dae-Ok , Kang Tong Ho , Lee Dae Young , Baek Nam-In
Published: October 2021

(1) Background: Many flavonoids have been reported to exhibit pharmacological activity; a preparatory study confirmed that Coreopsis lanceolata flowers (CLFs) contained high flavonoid structure content; (2) Methods: CLFs were extracted in aqueous methanol (MeOH:H2O = 4:1) and fractionated into acetic ester (EtOAc), normal butanol (n-BuOH), and H2O fractions. Repeated column chromatographies for two fractions led to the isolation of two aurones and two flavonols; (3) Results: Four flavonoids were identified based on a variety of spectroscopic data analyses to be leptosidin (1), leptosin (2), isoquercetin (3), and astragalin (4), respectively. This is the first report for isolation of 2–4 from CLFs. High-performance liquid chromatography (HPLC) analysis determined the content levels of compounds 1–4 in the MeOH extract to be 2.8 ± 0.3 mg/g (1), 17.9 ± 0.9 mg/g (2), 3.0 ± 0.2 mg/g (3), and 10.9 ± 0.9 mg/g (4), respectively. All isolated compounds showed radical scavenging activities and recovery activities in Caco-2, RAW264.7, PC-12, and HepG2 cells against reactive oxygen species. MeOH extract, EtOAc fraction, and 1–3 suppressed NO formation in LPS-stimulated RAW 264.7 cells and decreased iNOS and COX-2 expression. Furthermore, all compounds recovered the pancreatic islets damaged by alloxan treatment in zebrafish; (4) Conclusions: The outcome proposes 1–4 to serve as components of CLFs in standardizing anti-oxidant, pro-inflammatory inhibition, and potential anti-diabetic agents.
Broadband and Highly Directional Visible Light Scattering by Laser-Splashed Lossless TiO2 Nanoparticles
Authors: Zhang Yinan , Chen Shiren , Han Jing
Published: October 2021

All-dielectric nanoparticles, as the counterpart of metallic nanostructures have recently attracted significant interest in manipulating light-matter interaction at a nanoscale. Directional scattering, as an important property of nanoparticles, has been investigated in traditional high refractive index materials, such as silicon, germanium and gallium arsenide in a narrow band range. Here in this paper, we demonstrate that a broadband forward scattering across the entire visible range can be achieved by the low loss TiO2 nanoparticles with moderate refractive index. This mainly stems from the optical interferences between the broadband electric dipole and the magnetic dipole modes. The forward/backward scattering ratio reaches maximum value at the wavelengths satisfying the first Kerker’s condition. Experimentally, the femtosecond pulsed laser was employed to splash different-sized nanoparticles from a thin TiO2 film deposited on the glass substrate. Single particle scattering measurement in both the forward and backward direction was performed by a homemade confocal microscopic system, demonstrating the broadband forward scattering feature. Our research holds great promise for many applications such as light harvesting, photodetection and on-chip photonic devices and so on.
Recent Advances in Understanding the Role of Cartilage Lubrication in Osteoarthritis
Authors: Li Yumei , Yuan Zhongrun , Yang Hui , Zhong Haijian , Peng Weijie , Xie Renjian
Published: October 2021

The remarkable lubrication properties of normal articular cartilage play an essential role in daily life, providing almost frictionless movements of joints. Alterations of cartilage surface or degradation of biomacromolecules within synovial fluid increase the wear and tear of the cartilage and hence determining the onset of the most common joint disease, osteoarthritis (OA). The irreversible and progressive degradation of articular cartilage is the hallmark of OA. Considering the absence of effective options to treat OA, the mechanosensitivity of chondrocytes has captured attention. As the only embedded cells in cartilage, the metabolism of chondrocytes is essential in maintaining homeostasis of cartilage, which triggers motivations to understand what is behind the low friction of cartilage and develop biolubrication-based strategies to postpone or even possibly heal OA. This review firstly focuses on the mechanism of cartilage lubrication, particularly on boundary lubrication. Then the mechanotransduction (especially shear stress) of chondrocytes is discussed. The following summarizes the recent development of cartilage-inspired biolubricants to highlight the correlation between cartilage lubrication and OA. One might expect that the restoration of cartilage lubrication at the early stage of OA could potentially promote the regeneration of cartilage and reverse its pathology to cure OA.
Intrinsically Elastic Organic Semiconductors (IEOSs)
Authors: Liu Fei , Hou Xueling , Hu Benlin , Li Runwei
Published: October 2021

Elastic semiconductors are becoming more and more important to the development of flexible wearable electronic devices, which can be prepared by structural engineering design, blending, and the intrinsic elastification of organic semiconductors (intrinsically elastic organic semiconductor, IEOS). Compared with the elastic semiconductors prepared by structural engineering and blending, the IEOS prepared by organic synthesis has attracted numerous attentions for its solution processability and highly tunable chemical structures. For IEOSs, reasonable designs of synthetic routes and methods are the basis for realizing good mechanical and electrical properties. This brief review begins with a concise introduction of elastic semiconductors, then follows with several synthetic methods of IEOSs, and concludes the characteristics of each method, which provides guidance for the synthesis of IEOSs in the future. Furthermore, the properties of IEOSs are involved from the aspects of electrical, mechanical properties, and the applications of the IEOSs in elastic electronic devices. Finally, the challenge and an outlook which IEOSs are facing are presented in conclusion.
Case Study on the Fitting Method of Typical Objects
Authors: Zhang Liu , Zhang Jiakun , Song Hongzhen , Zhang Wen , Wang Wenhua
Published: October 2021

This study proposes different fitting methods for different types of targets in the 400–900 nm wavelength range, based on convex optimization algorithms, to achieve the effect of high-precision spectral reconstruction for small space-borne spectrometers. This article first expounds on the mathematical model in the imaging process of the small spectrometer and discretizes it into an AX=B matrix equation. Second, the design basis of the filter transmittance curve is explained. Furthermore, a convex optimization algorithm is used, based on 50 filters, and appropriate constraints are added to solve the target spectrum. First, in terms of spectrum fitting, six different ground object spectra are selected, and Gaussian fitting, polynomial fitting, and Fourier fitting are used to fit the original data and analyze the best fit of each target spectrum. Then the transmittance curve of the filter is equally divided, and the corresponding AX=B discrete equation set is obtained for the specific object target, and a random error of 1% is applied to the equation set to obtain the discrete spectral value. The fitting is performed for each case to determine the best fitting method with errors. Subsequently, the transmittance curve of the filter with the detector characteristics is equally divided, and the corresponding AX=B discrete equation set is obtained for the specific object target. A random error of 1% is applied to the equation set to obtain the error. After the discrete spectral values are obtained, the fitting is performed again, and the best fitting method is determined. In order to evaluate the fitting accuracy of the original spectral data and the reconstruction accuracy of the calculated discrete spectrum, the three evaluation indicators MSE, ARE, and RE are used for evaluation. To measure the stability and accuracy of the spectral reconstruction of the fitting method more accurately, it is necessary to perform 500 cycles of calculations to determine the corresponding MSE value and further analyze the influence of the fitting method on the reconstruction accuracy. The results show that different fitting methods should be adopted for different ground targets under the error conditions. The three indicators, MSE, ARE, and RE, have reached high accuracy and strong stability. The effect of high-precision reconstruction of the target spectrum is achieved. This article provides new ideas for related scholars engaged in hyperspectral reconstruction work and promotes the development of hyperspectral technology.
Study of an Integration Platform Based on an Adiabatic Active-Layer Waveguide Connection for InP Photonic Device Integration Mirroring That of Heterogeneous Integration on Silicon
Authors: Chen Changming , Li Junyu , Wang Chunxue , Huang Yingyan , Zhang Daming , Shi Zuosen , Cui Zhanchen , Yi Fei , Ho Seng-Tiong
Published: October 2021

In this work, a photonic device integration platform capable of integration of active-passive InP-based photonic devices without the use of material regrowth is introduced. The platform makes use of an adiabatic active-layer waveguide connection (ALWC) to move an optical beam between active and passive devices. The performance of this platform is analyzed using an example made up of four main sections: (1) a fiber coupling section for enabling vertical beam coupling from optical fiber into the photonic chip using a mode-matched surface grating with apodized duty cycles; (2) a transparent waveguide section for realizing passive photonic devices; (3) an adiabatic mode connection structure for moving the optical beam between passive and active device sections; and (4) an active device section for realizing active photonic devices. It is shown that the coupled surface grating, when added with a bottom gold reflector, can achieve a high chip-to-fiber coupling efficiency (CE) of 88.3% at 1550 nm. The adiabatic active-layer mode connection structure has an optical loss of lower than 1% (CE > 99%). The active device section can achieve an optical gain of 20 dB/mm with the use of only 3 quantum wells. The optimized structural parameters of the entire waveguide module are analyzed and discussed.
Biochar Amendments Improve Licorice (Glycyrrhiza uralensis Fisch.) Growth and Nutrient Uptake under Salt Stress
Authors: Egamberdieva Dilfuza , Ma Hua , Alaylar Burak , Zoghi Zohreh , Kistaubayeva Aida , Wirth Stephan , Bellingrath-Kimura Sonoko Dorothea
Published: October 2021

Licorice (Glycyrrhiza uralensis Fisch.) is a salt and drought tolerant legume suitable for rehabilitating abandoned saline lands, especially in dry arid regions. We hypothesized that soil amended with maize-derived biochar might alleviate salt stress in licorice by improving its growth, nutrient acquisition, and root system adaptation. Experiments were designed to determine the effect of different biochar concentrations on licorice growth parameters, acquisition of C (carbon), nitrogen (N), and phosphorus (P) and on soil enzyme activities under saline and non-saline soil conditions. Pyrolysis char from maize (600 °C) was used at concentrations of 2% (B2), 4% (B4), and 6% (B6) for pot experiments. After 40 days, biochar improved the shoot and root biomass of licorice by 80 and 41% under saline soil conditions. However, B4 and B6 did not have a significant effect on shoot growth. Furthermore, increased nodule numbers of licorice grown at B4 amendment were observed under both non-saline and saline conditions. The root architectural traits, such as root length, surface area, project area, root volume, and nodulation traits, also significantly increased by biochar application at both B2 and B4. The concentrations of N and K in plant tissue increased under B2 and B4 amendments compared to the plants grown without biochar application. Moreover, the soil under saline conditions amended with biochar showed a positive effect on the activities of soil fluorescein diacetate hydrolase, proteases, and acid phosphomonoesterases. Overall, this study demonstrated the beneficial effects of maize-derived biochar on growth and nutrient uptake of licorice under saline soil conditions by improving nodule formation and root architecture, as well as soil enzyme activity.
A Low Level of NaCl Stimulates Plant Growth by Improving Carbon and Sulfur Assimilation in Arabidopsis thaliana
Authors: Hongqiao Li , Suyama Akiko , Mitani-Ueno Namiki , Hell Ruediger , Maruyama-Nakashita Akiko
Published: October 2021

High-salinity stress represses plant growth by inhibiting various metabolic processes. In contrast to the well-studied mechanisms mediating tolerance to high levels of salt, the effects of low levels of salts have not been well studied. In this study, we examined the growth of Arabidopsis thaliana plants under different NaCl concentrations. Interestingly, both shoot and root biomass increased in the presence of 5 mM NaCl, whereas more than 10 mM NaCl decreased plant biomass. To clarify the biological mechanism by which a low level of NaCl stimulated plant growth, we analyzed element accumulation in plants grown under different NaCl concentrations. In addition to the Na and Cl contents, C, S, Zn, and Cu contents were increased under 5 mM NaCl in shoots; this was not observed at higher NaCl concentrations. Adverse effects of high salinity, such as decreased levels of nitrate, phosphate, sulfate, and some cations, did not occur in the presence of 5 mM NaCl. An increase in C was possibly attributed to increased photosynthesis supported by Cl, Zn, and Cu, which also increased in shoots after NaCl application. Salt stress-responsive gene expression was enhanced under 20 mM NaCl but not at lower doses. Among the S metabolites analyzed, cysteine (Cys) was increased by 5 mM NaCl, suggesting that S assimilation was promoted by this dose of NaCl. These results indicate the usefulness of NaCl for plant growth stimulation.
Genome-Wide Identification and Expression Analysis of MADS-Box Family Genes in Litchi (Litchi chinensis Sonn.) and Their Involvement in Floral Sex Determination
Authors: Guan Hongling , Wang Han , Huang Jianjun , Liu Mingxin , Chen Ting , Shan Xiaozhen , Chen Houbin , Shen Jiyuan
Published: October 2021

Litchi possesses unique flower morphology and adaptive reproduction strategies. Although previous attention has been intensively devoted to the mechanisms underlying its floral induction, the molecular basis of flower sex determination remains largely unknown. MADS-box genes are promising candidates for this due to their significant roles in various aspects of inflorescence and flower organogenesis. Here, we present a detailed overview of phylogeny and expression profiles of 101 MADS-box genes that were identified in litchi. These LcMADSs are unevenly located across the 15 chromosomes and can be divided into type I and type II genes. Fifty type I MADS-box genes are subdivided into Mα, Mβ and Mγ subgroups, while fifty-one type II LcMADSs consist of 37 MIKCC -type and 14 MIKC *-type genes. Promoters of both types of LcMADS genes contain mainly ABA and MeJA response elements. Tissue-specific and development-related expression analysis reveal that LcMADS51 could be positively involved in litchi carpel formation, while six MADS-box genes, including LcMADS42/46/47/75/93/100, play a possible role in stamen development. GA is positively involved in the sex determination of litchi flowers by regulating the expression of LcMADS51 (LcSTK). However, JA down-regulates the expression of floral organ identity genes, suggesting a negative role in litchi flower development.
Efficient Video-based Vehicle Queue Length Estimation using Computer Vision and Deep Learning for an Urban Traffic Scenario
Authors: Umair Muhammad , Farooq Muhammad Umar , Raza Rana Hammad , Chen Qian , Abdulhai Baher
Published: October 2021

In the Intelligent Transportation System (ITS) realm, queue length estimation is one of an essential yet a challenging task. Queue lengths are important for determining traffic density in traffic lanes so that possible congestion in any lane can be minimized. Smart roadside sensors such as loop detectors, radars and pneumatic road tubes etc. are promising for such tasks though they have a very high installation and maintenance cost. Large scale deployment of surveillance cameras have shown a great potential in the collection of vehicular data in a flexible way and are also cost effective. Similarly, vision-based sensors can be used independently or if required can also augment the functionality of other roadside sensors to effectively process queue length at prescribed traffic lanes. In this research, a CNN-based approach for estimation of vehicle queue length in an urban traffic scenario using low-resolution traffic videos is proposed. The queue length is estimated based on count of total vehicles waiting on a signal. The proposed approach calculates queue length without the knowledge of any onsite camera calibration information. Average vehicle length is approximated to be 5 m. This caters for the vehicles at the far end of the traffic lane that appear smaller in the camera view. Identification of stopped vehicles is done using Deep SORT based object tracking. Due to robust and accurate CNN-based detection and tracking, the queue length estimated by using only the cameras has been very effective. This mostly eliminates the need for fusion with any roadside or in-vehicle sensors. A detailed comparative analysis of vehicle detection models including YOLOv3, YOLOv4, YOLOv5, SSD, ResNet101, and InceptionV3 was performed. Based on this analysis, YOLOv4 was selected as a baseline model for queue length estimation. Using the pre-trained 80-classes YOLOv4 model, an overall accuracy of 73% and 88% was achieved for vehicle count and vehicle count-based queue length estimation, respectively. After fine-tuning of model and narrowing the output classes to vehicle class only, an average accuracy of 83% and 93% was achieved, respectively. This shows the efficiency and robustness of the proposed approach.
Enhanced Cell Performance and Improved Catalyst Utilization for a Direct Methanol Fuel Cell with an In-Plane Gradient Loading Catalyst Electrode
Authors: Chang Zhixin , Zhang Jiajia , Zhang Weiqi , Su Huaneng , Xing Lei , Ma Qiang , Zhang Hong , Xu Qian
Published: October 2021

Direct methanol fuel cells (DMFCs) offer high energy density, simple liquid fuel storage, and the ability to operate at ambient temperature. They may be used in a variety of portable mobile power supplies, small civilian power supplies, and automotive power supplies. However, in the process of electrochemical reaction inside a DMFC, because the reactants and products are distributed unevenly, the in-plane concentration of reactants and reaction rate are different; thus, the current density generated in the active area shows a high degree of non-uniformity. The high local current density can easily lead to the acceleration of DMFC aging. As a result, the operating cost of the DMFC is increased and the service life is shortened, which limits the commercial application of DMFCs. In this work, we develop an in-plane gradient loading catalyst. The loading on both the anode and cathode catalysts was lower near the inlet and higher close to the outlet. The experimental results of the single-cell test show that the performance of the gradient loading catalyst electrode was enhanced by up to 19.8% compared with the uniform loading catalyst at 60 °C for the same catalyst loading, especially under high current densities. In addition, the catalyst utilization was improved for the gradient loading catalyst electrode. Hence, the proposed approach shows potential for reducing the cost and increasing the service life of DMFCs.
Distribution of Nanoparticles in a Turbulent Taylor–Couette Flow Considering Particle Coagulation and Breakage
Authors: Shi Ruifang , Lin Jianzhong , Yang Hailin
Published: October 2021

In this paper, the dynamic evolution of nanoparticles in a turbulent Taylor–Couette flow was studied by means of a numerical simulation. The initial particle size was 200 nm, and the volume concentration was 1 × 10−5. The Reynolds-averaged N–S equation for Taylor–Couette flow was solved numerically using the realizable k-ε model combined with the standard wall function. The numerical result of the velocity distribution is in good agreement with the experimental results. Additionally, the dynamic equation for the particle number distribution function was solved numerically using the Taylor series expansion moment method (TEMOM). The variation characteristics of particle number density, diameter and polydispersity in the flow were analyzed. The results show that particle breakage is obvious in the region with strong vorticity due to the large shear strength, which leads to a significant change in the particle number density, diameter and polydispersity. Furthermore, the effects of the gap width between two cylinders and the Reynolds number on the distribution of the particle number density, size and polydispersity are discussed.
A Study of Distributed Earth Observation Satellites Mission Scheduling Method Based on Game-Negotiation Mechanism
Authors: Liu Lihao , Dong Zhenghong , Su Haoxiang , Yu Dingzhan
Published: October 2021

While monolithic giant earth observation satellites still have obvious advantages in regularity and accuracy, distributed satellite systems are providing increased flexibility, enhanced robustness, and improved responsiveness to structural and environmental changes. Due to increased system size and more complex applications, traditional centralized methods have difficulty in integrated management and rapid response needs of distributed systems. Aiming to efficient missions scheduling in distributed earth observation satellite systems, this paper addresses the problem through a networked game model based on a game-negotiation mechanism. In this model, each satellite is viewed as a “rational” player who continuously updates its own “action” through cooperation with neighbors until a Nash Equilibria is reached. To handle static and dynamic scheduling problems while cooperating with a distributed mission scheduling algorithm, we present an adaptive particle swarm optimization algorithm and adaptive tabu-search algorithm, respectively. Experimental results show that the proposed method can flexibly handle situations of different scales in static scheduling, and the performance of the algorithm will not decrease significantly as the problem scale increases; dynamic scheduling can be well accomplished with high observation payoff while maintaining the stability of the initial plan, which demonstrates the advantages of the proposed methods.
Enantioselective Chromatographic Separation and Lipase Catalyzed Asymmetric Resolution of Biologically Important Chiral Amines
Authors: El-Behairy Mohammed Farrag , Hassan Rasha M. , Sundby Eirik
Published: October 2021

Cyanoacetamides are vital synthons in synthetic organic chemistry. However, methods to enantiopure cyanoacetamides have not yet been well explored. In this work, the preparation of cyanoacetamide synthons RS-(1a–4a) or methoxyacetamides RS-(1b–4b) in enantiopure/enriched form was investigated. Compounds S-1, S-2, R-1b, R-1a, andR-2b were prepared in enantiopure form (ee > 99%) while compounds S-4, R-2a, and R-4a were achieved in ee 9%, 80%, and 76%, respectively. Many baselines enantioselective HPLC separations of amines 1–4, their cyanoacetamides (1a–4a), and methoxyacetamides (1b–4b) were achieved by utilizing diverse mobile-phase compositions and two cellulose-based CSPs (ODH® and LUX-3® columns). Such enantioselective HPLC separations were used to monitor the lipase-catalyzed kinetic resolution of amines RS-(1–4).
Influence of Harvest Time, Method of Preparation and Method of Distillation on the Qualitative Properties of Organically Grown and Wild Helichrysum italicum Immortelle Essential Oil
Authors: Matin Ana , Pavkov Ivan , Grubor Mateja , Jurišić Vanja , Kontek Mislav , Jukić Franko , Krička Tajana
Published: October 2021

The most important process in immortelle is the extraction of the essential oil by distillation. The chemical properties of the plant depend largely on the cultivation type, climatic conditions and agrotechnical measures. In this work, studies were carried out on organically grown and wild immortelle harvested during the summer (July) and autumn (October) seasons of 2020. Immortelle samples were prepared by drying naturally in two ways: in direct sunlight and in shade. To extract the essential oil, immortelle was distilled in two ways: on an industrial and a laboratory scale. The essential oil was analyzed by gas chromatography to determine the composition and quality of the essential oil. The results of this work showed that the qualitative properties of the essential oil were influenced by the cultivation type, harvesting time, preparation method and distillation method. A higher yield (0.21% ± 0.03) of essential oil was obtained from wild immortelle whereas a better utilization rate (0.38% ± 0.09) of essential oil was obtained from the organically grown immortelle. It was also found that a higher yield of essential oil was obtained from the summer harvest (0.19% ± 0.01) and a higher utilization rate from the autumn harvest (0.33% ± 0.13) with the industrial scale distillation and shade drying. In general, better qualitative properties of immortelle essential oil were obtained from wild immortelle, which was confirmed by a chromatographic analysis and better content of γ-curcumene (16.64% ± 0.30) and neryl acetate (8.15% ± 0.19) and other constituents except α-pinene where organically grown had a higher share (24.27% ± 0.97).
Accurate Determination of Electrical Potential on Ion Exchange Membranes in Reverse Electrodialysis
Authors: Sun Yuting , Song Lianfa
Published: October 2021

Reverse electrodialysis is a promising membrane technology to generate energy from controlled mixing of water streams of different salinities. Electrical potentials generate on the ion exchange membranes (IEMs) when selective transport of cations and anions across the membranes driven by concentration difference. The accurate determination of the potentials developed on the IEMs is critical to fairly assess the feasibility of the technology. The Nernst–Planck–Poisson (NPP) equations for IEMs (the membranes with fixed charge) were solved numerically with the boundary updating scheme. The validity of this numerical method was verified by the identical values of Donnan potential obtained with the well-established analytical methods. The suitability and applicability of the classic Teorell–Meyer–Siever (TMS) model were assessed by comparison to the simulation results from the numerical method.
Tyrosol and Hydroxytyrosol Determination in Extra Virgin Olive Oil with Direct Liquid Electron Ionization-Tandem Mass Spectrometry
Authors: Termopoli Veronica , Piergiovanni Maurizio , Cappiello Achille , Palma Pierangela , Famiglini Giorgio
Published: October 2021

Extra virgin olive oil (EVOO) is one of the main ingredients of the Mediterranean diet. It is claimed as a functional food for its unique content of health-promoting compounds. Tyrosol (Tyr), Hydroxytyrosol (Htyr), and their phenolic derivatives present in EVOO show beneficial properties, and their identification and quantification, both in their free form and after the hydrolysis of more complex precursors, are important to certify its quality. An alternative method for quantifying free and total Tyr and Htyr in EVOO is presented using an LC–MS interface based on electron ionization (EI), called liquid electron ionization (LEI). This method requires neither sample preparation nor chromatography; the sample is diluted and injected. The selectivity and sensitivity were assessed in multiple reaction monitoring mode (MRM), obtaining confirmation and quantification in actual samples ranging from 5 to 11 mg/Kg for the free forms and from 32 to 80 mg/Kg for their total amount after hydrolysis. Two MS/MS transitions were acquired for both compounds using the Q/q ratios as confirmatory parameters. Standard addition calibration curves demonstrated optimal linearity and negligible matrix effects, allowing a correct quantification even without expensive and difficult to find labeled internal standards. After several weeks of operation, the system’s repeatability was excellent, with an intraday RSD (%) spanning from five to nine and an interday RSD (%) spanning from 9 to 11.
Nutritional Value of Sea Urchin Roe (Strongylocentrotidae)—Study of Composition and Storage Conditions
Authors: Matveeva Victoria A. , Shulgina Lidia V. , Prikhodko Yury V. , Shulgin Yury P. , Madej Katarzyna , Piekoszewski Wojciech
Published: October 2021

Although the roe of sea urchins inhabiting the Far Eastern seas possesses many healing properties and may be used as a dietary product, a reduction and deterioration in its nutritional quality during storage occurs. Therefore, in order to make sea urchin products widely accessible to the world population, it is very important to have appropriate technology to keep the roe from spoiling. To store sea urchin roe for a long time, methods of pre-processing sea urchin gonads before freezing were tested. In terms of preserving organoleptic properties and nutritional quality, the most adequate procedure consists of a short period (20 or 30 s) of heat (boiling water) treatment of sea urchin roe after removal from the shell. This procedure results in an inactivation of enzymes that catalyze the hydrolytic processes of lipids and proteins during storage. After blanching and cooling, the roe was packed, frozen and kept at a temperature of −18 °C and −25 °C. The quality of sea urchin roe did not change during storage at the temperature of −18 °C for 6 months, and at the temperature of −25 °C for 10 months.
Deep Eutectic Solvents (DESs) as Green Extraction Media of Beneficial Bioactive Phytochemicals
Authors: Dheyab Ali Sami , Abu Bakar Mohd Fadzelly , AlOmar Mohamed , Sabran Siti Fatimah , Muhamad Hanafi Ahmad Fathi , Mohamad Azman
Published: October 2021

Deep eutectic solvents (DES) are a mixture of two or more components and are classified as ionic solvents with special properties such as low volatility, high solubility, low melting points, low-cost materials and are less toxic to humans. Using DES has been suggested as an eco-friendly, green method for extraction of bioactive compounds from medicinal plants and are a safe alternative for nutritional, pharmaceutical and various sector applications. Conventional solvent extraction methods present drawbacks such as long extraction period, safety issues, harmful to the environment, costly and large volume of solvents required. The extraction method with DES leads to higher extraction yield and better bioactivity results as compared to the conventional solvents. This review provides a summary of research progress regarding the advantages of using DES to extract bioactive compounds such as phenolic acid, flavonoids, isoflavones, catechins, polysaccharides, curcuminoids, proanthocyanidin, phycocyanin, gingerols, ginsenosides, anthocyanin, xanthone, volatile monoterpenes, tannins, lignin, pectin, rutin, tert-butyl hydroquinone, chlorogenic acids, resveratrol and others, as opposed to using conventional solvents. The bioactivity of the extracts is determined using antioxidant, antibacterial and antitumor activities. Hence, DESs are considered potential green media with selective and efficient properties for extracting bioactive ingredients from medicinal plants.