bluuebunny/crossref_metadata_2025 huggingface dataset:
The Mediation Role of Safety Attitude in the Impact of Resilience on the Safety Behavior of Coal Miners in China
Authors: Li Yuanlong , Gao Jingqi , Qian Chongyang , Wu Xiang
Published: November 2022

Resilience can improve the adaptability of coal miners to high-hazard and high-stress environments. After facing setbacks or adversities, resilience can enable coal miners to recover from bad mental states and have an optimistic safety attitude and positive safety behaviors. However, how resilience affects safety behavior and the role of safety attitude in the relationship have not been clear. This study systematically reviewed previous research on resilience, safety attitude, and safety behavior. By recovering 639 valid questionnaires, the validity and reliability of the resilience scale, safety attitude scale, and safety behavior scale for coal miners were verified. Hierarchical regression analysis explored the relationships between resilience, safety attitude, and safety behavior. Studies have shown that resilience positively affects safety attitude and safe behavior. Safety attitude positively affects safety behaviors and plays a role as a partial mediator in the impact of resilience on safe behavior. The theoretical contribution is that the resilience of miners has a positive impact on safety behavior. Moreover, resilience can also act on safety behaviors through the partial intermediation of safety attitude. The practical contribution is that managers of coal mining companies can promote the resilience and safety attitude of coal miners to improve safety behaviors and prevent accidents.
Characteristics, Components, and Efficacy of Telerehabilitation Approaches for People with Chronic Obstructive Pulmonary Disease: A Systematic Review and Meta-Analysis
Authors: Isernia Sara , Pagliari Chiara , Bianchi Luca Nicola Cesare , Banfi Paolo Innocente , Rossetto Federica , Borgnis Francesca , Tavanelli Monica , Brambilla Lorenzo , Baglio Francesca
Published: November 2022

Introduction: Chronic obstructive pulmonary disease (COPD) is at the top of the list of non-communicable diseases with related rehabilitation needs. Digital medicine may provide continuative integrated intervention, overcoming accessibility and cost barriers. Methods: We systematically searched for randomized controlled trials on telerehabilitation (TR) in people with COPD to profile the adopted TR strategies, focusing on TR models and the main rehabilitation actions: monitoring and assessment, decision, and feedback. Additionally, a meta-analysis was run to test the TR effect on functional capacity, dyspnea, and quality of life compared to no intervention (NI) and conventional intervention (CI). Results: Out of the 6041 studies identified, 22 were eligible for the systematic review, and 14 were included in the meta-analyses. Results showed a heterogeneous scenario in terms of the TR features. Furthermore, only a small group of trials presented a comprehensive technological kit. The meta-analysis highlighted a significant effect of TR, especially with the asynchronous model, on all outcomes compared to NI. Moreover, a non-inferiority effect of TR on functional capacity and quality of life, and a superiority effect on dyspnea compared to CI were observed. Finally, the studies suggested a high rate of TR adherence and high safety level. Conclusions: TR is an effective strategy to increase and maintain functional capacity, breath, and quality of life in people with COPD. However, a consensus on the essential elements and features of this approach needs to be defined, and the effect of long-term maintenance merits further investigation.
Measuring Health Literacy in Basic Education in Finland: The Development of a Curriculum- and Performance-Based Measurement Instrument
Authors: Summanen Anna-Mari , Rautopuro Juhani , Kannas Lasse , Paakkari Leena
Published: November 2022

This paper describes the development of an objective curriculum- and performance-based health literacy (HL) measurement instrument to assess Finnish 9th graders’ learning outcomes in the school subject termed Health Education (HE). There were four phases: (i) construction of the theoretical framework for the measurement, (ii) item generation, (iii) the field test (n = 252), and (iv) item analysis and item selection for the main study, in which 3652 ninth grade pupils (aged 15–16) participated. Initially, 303 HL test items were formulated, of which 107 were tested in two different field test versions. Both versions exhibited high reliability as measured by Cronbach’s alpha coefficient. The main study contained 55 items. Testing and item analysis enabled the development of a comprehensive competence- and curriculum-based HL measurement instrument for school-aged children. Measurement of HL in schools provides information for national policies, and for developing HE as a school subject.
Risk Association of Liver Cancer and Hepatitis B with Tree Ensemble and Lifestyle Features
Authors: Koh Eunji , Kim Younghoon
Published: November 2022

The second-largest cause of death by cancer in Korea is liver cancer, which leads to acute morbidity and mortality. Hepatitis B is the most common cause of liver cancer. About 70% of liver cancer patients suffer from hepatitis B. Early risk association of liver cancer and hepatitis B can help prevent fatal conditions. We propose a risk association method for liver cancer and hepatitis B with only lifestyle features. The diagnostic features were excluded to reduce the cost of gathering medical data. The data source is the Korea National Health and Nutrition Examination Survey (KNHANES) from 2007 to 2019. We use 3872 and 4640 subjects for liver cancer and hepatitis B model, respectively. Random forest is employed to determine functional relationships between liver diseases and lifestyle features. The performance of our proposed method was compared with six machine learning methods. The results showed the proposed method outperformed the other methods in the area under the receiver operator characteristic curve of 0.8367. The promising results confirm the superior performance of the proposed method and show that the proposed method with only lifestyle features provides significant advantages, potentially reducing the cost of detecting patients who require liver health care in advance.
Genetic Associations of Visfatin Polymorphisms with EGFR Status and Clinicopathologic Characteristics in Lung Adenocarcinoma
Authors: Chang Sunny Li-Yun , Yang Po-Jen , Lin Yen-You , Jiang Ya-Jing , Liu Po-I , Huang Chang-Lun , Yang Shun-Fa , Tang Chih-Hsin
Published: November 2022

Lung adenocarcinoma (LUAD) is the most common histologic type of lung cancer. Mutations of the epidermal growth factor receptor (EGFR) gene are among the most common genetic alterations in LUAD and are the targets of EGFR tyrosine kinase inhibitors. The enzyme visfatin is involved in the generation of the oxidized form of nicotinamide adenine dinucleotide (NAD+) and regulation of intracellular adenosine triphosphate (ATP), critical processes in cancer cell survival and growth. This study explored the relationship between visfatin single nucleotide polymorphisms (SNPs) with EGFR status and the clinicopathologic development of LUAD in a cohort of 277 Taiwanese men and women with LUAD. Allelic discrimination of four visfatin SNPs rs11977021, rs61330082, rs2110385 and rs4730153 was determined using a TaqMan Allelic Discrimination assay. We observed higher prevalence rates of advanced (T3/T4) tumors and distant metastases in EGFR wild-type patients carrying the rs11977021 CT + TT and rs61330082 GA + AA genotypes, respectively, compared with patients carrying the CC and GG genotypes. EGFR wild-type patients carrying the rs11977021 CT + TT genotypes were also more likely to develop severe (stage III/IV) malignancy compared with patients carrying the CC genotype. An analysis that included all patients found that the association persisted between the rs11977021 CT + TT and rs61330082 GA + AA genotypes and the development of T3/T4 tumors compared with patients carrying the rs11977021 CC and rs61330082 GG genotypes. In conclusion, these data indicate that visfatin SNPs may help to predict tumor staging in LUAD, especially in patients with EGFR wild-type status.
Value of Online Videos as a Shoulder Injection Training Tool for Physicians and Usability of Current Video Evaluation Tools
Authors: Jang Chan Woong , Bang Myeonghwan , Park Jung Hyun , Cho Han Eol
Published: November 2022

This study aimed to evaluate the reliability, overall quality, and educational value of online videos for learning the techniques related to shoulder injection treatments and analyzing the usability of video evaluation tools for musculoskeletal injections. Online video searches were performed in February 2022 using the terms “shoulder injection”, “glenohumeral joint injection”, “acromioclavicular joint injection”, and “subacromial bursa injection.” Included videos were scored by modified DISCERN (mDISCERN), global quality score (GQS), and shoulder injection score (SIS). Correlations between scoring systems were analyzed. Of the 150 videos, 49 (32.67%) contained highly reliable information. Regarding the assessment of overall quality by the GQS, 109 (72.67%) videos were of low quality. Regarding SIS, 114 (76.00%) scored not >5, of which 77 (51.33%) scored <3. Most of the SIS domains were fully explained in <40% of the included videos. A weak positive relationship was noted between the mDISCERN and SIS (r2 = 0.38), while a moderately positive relationship was observed between the GQS and SIS (r2 = 0.49). The majority of online videos about shoulder injection treatment showed low reliability, overall quality, and educational value. Additionally, a new scoring system is required to accurately evaluate musculoskeletal injection videos for educational purposes.
Hazardous Chemicals Road Transportation Accidents and the Corresponding Evacuation Events from 2012 to 2020 in China: A Review
Authors: Zhang Weihua , Cheng Wuyi , Gai Wenmei
Published: November 2022

Based on accident data from the China Chemical Accident Information Network, detailed information was obtained from 2657 hazardous chemicals road transportation accidents (HCRTAs) and 148 evacuations caused by these accidents that occurred in China from 2012 to 2020. The characteristics and the development trend of the present HCRTAs in China and the rate of emergency are obtained via statistical analysis. Based on the probability of evacuation scenarios via historical statistics, the social cost of labor loss value of participating emergency responders, and evacuees’ placement and transfer cost as the consequences of evacuation events, an evacuation event grading model based on social risk assessment is constructed. Evaluating and classifying the risk of evacuation events caused by HCRTAs (148), the results demonstrated that the social risk caused by emergency scenarios F_61 (leakage due to overturning of hazardous chemical vehicles, which led to evacuation) and F_91 (leakage due to rear-end of hazardous chemical vehicles, which led to evacuation) was higher than other emergency scenarios. To reduce the dangers caused by HCRTAs, the framework for improving the emergency response capacity of communities is discussed and analyzed based on five aspects, which comprise land use planning, city construction, education promotion, information construction, and the layout of emergency resources.
Organic Food Consumption and Perception among Polish Mothers of Children under 6 Years Old
Authors: Woś Karolina , Dobrowolski Hubert , Gajewska Danuta , Rembiałkowska Ewa
Published: November 2022

Pro-environmental attitudes, including organic food consumption, can reduce negative impact on the environment. The consumption of organic food in Poland is rather low, but the ecological awareness of Poles is steadily increasing. The aim of the study was to assess the frequency and factors influencing the consumption of organic products and to analyze the perception of such food by mothers of children under 6 years of age (n = 667). The survey was conducted between March 2020 and May 2021 in three voivodships in Eastern Poland. The results of the survey indicate that the majority of respondents are occasional consumers of organic food (51%). The most commonly consumed organic products are eggs, vegetables and fruits, whereas the least consumed are alcoholic beverages, coffees and ready-to-eat meals. The responders’ main characteristics of organic food are as follows: no genetic modification/GMO-free, no synthetic additives and having organic certification. Statistically significant correlations were found between the frequency of organic food consumption and education, financial situation as well as familiarity with the logo of the organic certificate and verifying that it is present on the packaging. The most common reasons for consuming organic food were health issues, while the high price was declared as the main barrier.
Pharmaceutical Enterprises’ R&D Innovation Cooperation Moran Strategy When Considering Tax Incentives
Authors: Xu Yanping , Zhu Lilong
Published: November 2022

Drug R&D innovation contributes to the high-quality development of the pharmaceutical industry, which is related to people’s life and health, economic development, and social stability. Tax incentives and industry cooperation are conducive to promoting pharmaceutical enterprises’ innovation. Therefore, this paper constructs a Moran process evolutionary game model and analyzes the evolutionary trajectory of N pharmaceutical enterprises’ drug R&D innovation strategic choice and considers the choice of R&D innovation strategy and non-R&D innovation strategy. We obtain the conditions for the two strategies to achieve evolutionary stability under the dominance of external factors, the dominance of expected revenue, and the dominance of super expected revenue. The evolutionary process is simulated by MATLAB 2021b. The results show that, firstly, when the number of pharmaceutical enterprises is higher than a threshold, the market is conducive to pharmaceutical enterprises choosing an R&D innovation strategy. Secondly, the higher the tax incentives, the higher the probability of pharmaceutical enterprises choosing an R&D innovation strategy. Thirdly, when the R&D success rate increases, pharmaceutical enterprises gradually change from choosing a non-R&D innovation strategy to choosing an R&D innovation strategy. Fourthly, the threshold of strategy change of pharmaceutical enterprises is the same under the dominance of expected revenue and super expected revenue. This paper puts forward some countermeasures and suggestions for promoting the R&D innovation of pharmaceutical enterprises in practice.
Admission to the Emergency Department by Patients Being Followed up for Palliative Care Consultations
Authors: Brites Mariana Azevedo , Gonçalves Joana , Rego Francisca
Published: November 2022

Introduction: Palliative care aims to improve the quality of life of patients and families facing life-threatening diseases. Admissions to the emergency department are considered potentially avoidable. This study aims to characterize the use of the emergency department by palliative care patients at a public hospital in Portugal. Methods: This retrospective study included patients who had their first palliative care appointment during the year 2019; 135 patients were included, with 255 admissions to the emergency department. Descriptive statistical analysis consisted of calculating the absolute (n) and relative (%) frequencies for categorical variables and medians (Mdn) and percentiles (P25 and P75) for continuous variables. The multivariable associations were calculated via logistic models, with the statistical significance set to p < 0.05 and 95% confidence intervals. Results: Dying in hospital was associated with going to the emergency department. Patients who died in hospital had more admissions and spent more time there. Conclusion: Emergency department admissions suggest that there are gaps in the provision of care. It is necessary to anticipate crisis situations, provide home and telephone appointments, and invest in professionals’ education to respond to the needs that will grow in the future.
Addressing Substance Use and Misuse in East Texas: Stakeholder-Driven Needs and Priorities
Authors: Tiruneh Yordanos M. , Elliott Kimberly S. , Oyer Linda , Elueze Emmanuel , Casanova Vanessa
Published: November 2022

Background—This project sought to assess needs, perceived challenges, and priorities regarding substance use disorder (SUD) in East Texas and develop a community-driven research agenda to address those challenges. Methods—Data were gathered through nine focus-group discussions (FGDs) with stakeholders: people living with SUD, families, medical providers, counselors, representatives of community-based organizations, and law enforcement officers. We asked participants how substance use manifests in their communities, which challenges they confronted in coping with substance use and misuse, and in which order their needs should be prioritized. Findings were reported at community forums to confirm the list of challenges and prioritize needs. Results—Five themes emerged from the FGDs indicating major challenges: (a) access to SUD treatment and recovery resources, (b) mental health and resiliency, (c) education, training, and professional development to facilitate treatments, (d) care and service coordination, and (e) community/social support for people living with SUD and their families. Conclusions—Significant resources such as financing, collaboration across silos, and community education are needed to effectively manage this public health problem. Our findings can inform research and outreach to help East Texans develop interventions, research programs, and educational opportunities for clinicians, community-based organizations, law enforcement officers, and counselors to build capacity for SUD prevention, treatment, and recovery.
Germinated Millet (Pennisetum glaucum (L.) R. Br.) Flour Improved the Gut Function and Its Microbiota Composition in Rats Fed with High-Fat High-Fructose Diet
Authors: Theodoro Jaqueline Maciel Vieira , Grancieri Mariana , Gomes Mariana Juste Contin , Toledo Renata Celi Lopes , de São José Vinícius Parzanini Brilhante , Mantovani Hilário Cuquetto , Carvalho Carlos Wanderlei Piler , da Silva Bárbara Pereira , Martino Hércia Stampini Duarte
Published: November 2022

Germinated millet (Pennisetum glaucum (L.) R. Br.) is a source of phenolic compounds that has potential prebiotic action. This study aims at evaluating the action of germinated pearl millet on gut function and its microbiota composition in Wistar rats fed with a high-fat high-fructose (HFHF) diet. In the first stage, lasting eight weeks, the experiment consisted of two groups: AIN-93M (n = 10) and HFHF group (n = 20). In the second stage, which lasted ten weeks, the animals of the AIN-93M group (n = 10) were kept, while the HFHF group was dismembered into HFHF (HFHF diet, n = 10) and HFHF + millet (HFHF added 28.6% of germinated millet flour, n = 10) groups. After the 18th week, the urine of the animals was collected for the analysis of lactulose and mannitol intestinal permeability by urinary excretion. The histomorphometry was analyzed on the proximal colon and the fecal pH, concentration of short-chain fatty acids (SCFA), and sequencing of microbiota were performed in cecum content. The Mothur v.1.44.3 software was used for data analysis of sequencing. Alpha diversity was estimated by Chao1, Shannon, and Simpson indexes. Beta diversity was assessed by PCoA (Principal Coordinate Analysis). The functional predictive analysis was performed with PICRUSt2 software (version 2.1.2−b). Functional traits attributed to normalized OTU abundance were determined by the Kyoto Encyclopedia of Genes and Genomes (KEGG). In the results, germinated millet flour reduced Oscillibacter genus and Desulfobacterota phylum, while increasing the Eggerthellaceae family. Furthermore, germinated millet flour: increased beta diversity, cecum weight, and cecum/body weight ratio; improved gut histological parameters by increasing the depth and thickness of the crypt and the goblet cell count (p < 0.05); reduced (p < 0.05) the fecal pH and mannitol urinary excretion; increased (p < 0.05) the propionate short-chain fatty acid concentration. Thus, germinated millet has the potential to improve the composition of gut microbiota and the intestinal function of rats fed with an HFHF diet.
Health-Related Factors Influencing Nurse Turnover by Clinical Career: A Secondary Data Analysis of Clinical Nurses in South Korea
Authors: Kang Jiwon , Lee Youngjin
Published: November 2022

The increase in clinical nurse turnover is an important issue in human resource management worldwide. Factors influencing it include health-related risk factors such as sleep quality and presenteeism, which need further exploration. We examined differences in job survival time of clinical nurses in relation to nurses’ sleep quality and presenteeism. Participants were 857 Korean clinical nurses with more than three months’ experience providing direct patient care. Data were analyzed using a time-independent Cox proportional hazard regression analysis of factors affecting actual turnover of clinical nurses. Average job survival times of competent, proficient, and expert nurses were 33, 64, and 143 months, respectively. Sleep quality and presentism significantly affected turnover risk. For clinical nurses with less than three years of experience, sleep quality significantly influenced the risk of turnover. For clinical nurses with more than six years of experience, presenteeism significantly affected the risk of turnover. The findings of this study offer a clinical career-based approach to reduce the turnover rate of clinical nurses. A differentiated approach based on work experience is necessary to establish a turnover management strategy for clinical nurses.
Development and Validation of a Novel Ultra-Compact and Cost-Effective Device for Basic Hands-On CPR Training: A Randomized, Sham-Controlled, Blinded Trial
Authors: Rabanales-Sotos Joseba , Guisado-Requena Isabel María , Leiton-Espinoza Zoila Esperanza , Guerrero-Agenjo Carmen María , López-Torres-Hidalgo Jesús , Martín-Conty José Luis , Martín-Rodriguez Francisco , López-Tendero Jaime , López-González Angel
Published: November 2022

To examine the performance of a novel low-cost, ultra-compact, and attractive auditory feedback device for training laypeople in external chest compressions (ECCs), we conducted a quasi-experimental cross-sectional study from September to November 2021 at the Faculty of Nursing of Albacete, University of Castille-La Mancha, Spain. The ECC sequence was performed in the laboratory with the new device for basic hands-on CPR training. Results: One hundred college students were included in this study. The compression rate/min with the new device was 97.6, and the adequate %ECC was 52.4. According to the status of body mass index (BMI) and muscle strength of the upper limbs in the bivariate analysis, it was observed that the new device discriminated between those who performed correct ECCs according to their BMI and muscle strength and those who did not, which led to significantly influenced results in terms of the percentage of ECCs with correct depth. Conclusions: The new ultra-compact auditory feedback device “Salvando a Llanetes®” demonstrated utility for teaching and learning ECCs in basic CPR. We can affirm that the analyzed device is an adequate, safe and economical method for teaching “CPR Hands-Only™” to the general population.
The Health Experiences of Young Internal Migrants in Ghana—Identifying Priorities for Sustainable Health Promotion
Authors: Spencer Grace , Dankyi Ernestina , Thompson Jill , Acton Faye , Kwankye Stephen Owusu
Published: November 2022

The Sustainable Development Goals underscore the importance of migration to the achievement of health, and global migration presents both opportunities and challenges for the development of health promotion. Despite such recognition, very little work has focused on health promotion with young migrants, including how migration shapes opportunities for positive health. This paper reports findings from a qualitative study that sought to advance knowledge of the health experiences of young internal migrants in Ghana (n = 14) and considers ways to harness these perspectives in the development of sustainable health promotion solutions. Methods included community consultations, participatory workshops and interviews with young migrants aged 14–21 years. Findings highlighted how the social determinants of health affected young migrants’ opportunities to support their health. Our analysis highlights how a lack of access to adequate food, shelter and health services often resulted in the adoption of alternative health practices, including the use of herbal remedies. Supporting positive livelihoods as part of tackling the social determinants of health is crucial to mitigate the impacts of poverty and inequalities on young migrants’ health practices and outcomes. We conclude by considering how to advance relevant health promotion with young migrants living in contexts of vulnerability.
Neck Circumference and Blood Pressure Measurements among Walter Sisulu University Students
Authors: Ramoshaba Nthai E. , Fihla Mthetho Q. , Mthethwa Wenzile S. , Tshangela Lisa , Mampofu Zuqaqambe M.
Published: November 2022

Hypertension is a public health problem in South Africa. Increases in subcutaneous fat, presented by the neck circumference (NC) value, contribute to and predict the development of hypertension. However, to date, there has been no study done to investigate the relationship between the NC and blood pressure (BP) among historically disadvantaged university students. Therefore, the aim of the study was to investigate the relationship between the NC and BP among Walter Sisulu University students. This cross-sectional study was conducted in Walter Sisulu University. All 127 students were aged 18 years and above and underwent NC and clinical BP measurements using standard procedures. In a Pearson’s correlation analysis, the NC positively correlated with both the systolic blood pressure (SBP) (r = 0.5; p < 0.001) and diastolic blood pressure (DBP) (r = 0.3; p < 0.001). Furthermore, in the multivariable-adjusted regression analysis, the NC was positively associated with both the SBP (adjusted R2 = 0.3, β = 2.0 (95% CI = 1.1; 2.9), p < 0.001) and DBP (adjusted R2 = 0.1 β = 0.950 (95% CI = 0.3; 1.6), p = 0.008) adjusted for age, gender, body mass index, waist-to-height ratio, alcohol, and smoking. The NC is related to BP among historically disadvantaged university students.
Evaluation of the Second Premolar’s Bud Position Using Computer Image Analysis and Neural Modelling Methods
Authors: Cieślińska Katarzyna , Zaborowicz Katarzyna , Zaborowicz Maciej , Biedziak Barbara
Published: November 2022

Panoramic radiograph is a universally used diagnostic method in dentistry for identifying various dental anomalies and assessing developmental stages of the dentition. The second premolar is the tooth with the highest number of developmental abnormalities. The purpose of this study was to generate neural models for assessing the position of the bud of the second premolar tooth based on analysis of tooth–bone indicators of other teeth. The study material consisted of 300 digital pantomographic radiographs of children in their developmental period. The study group consisted of 165 boys and 135 girls. The study included radiographs of patients of Polish nationality, aged 6–10 years, without diagnosed systemic diseases and local disorders. The study resulted in a set of original indicators to accurately assess the development of the second premolar tooth using computer image analysis and neural modelling. Five neural networks were generated, whose test quality was between 68–91%. The network dedicated to all quadrants of the dentition showed the highest test quality at 91%. The training, validation and test subsets were divided in a standard 2:1;1 ratio into 150 training cases, 75 test cases and 75 validation cases.
Perceived Provision of Perioperative Information and Care by Patients Who Have Undergone Surgery for Colorectal Cancer: A Cross-Sectional Study
Authors: Zucca Alison , Mansfield Elise , Sanson-Fisher Rob , Wyse Rebecca , Johnston Sally-Anne , Fakes Kristy , Robinson Sancha , Smith Stephen
Published: November 2022

Background: Active patient participation in preparation and recovery from colorectal cancer surgery can be facilitated by timely information and care and may improve patient wellbeing and reduce hospitalizations; Methods: We aimed to identify gaps in perioperative information and care by asking colorectal cancer surgical patients to retrospectively report on their perceptions of care via a cross-sectional survey; Results: Overall, 179 (64% consent rate) patients completed one of two 64-item surveys exploring their views of ‘optimal care’ or their experiences of ‘actual care’. In total, 41 (64%) aspects of care were endorsed as optimal. Of these, almost three-quarters (73%) were received by most patients (80% or more). Gaps in care were identified from discrepancies in the endorsement of optimal versus actual survey items. Of the 41 items identified as representing ‘optimal care’, 11 items were received by fewer than 80% of patients, including the provision of information about the impact of surgical wait-times on cancer cure (69%); pre-habilitation behaviors to improve health (75%); the type of questions to ask the health care team (74%); impact of pain medications on bowel movements (73%); how to obtain medical supplies for self-care at home (67%); dietary or exercise advice after discharge (25–31%); and emotional advice after discharge (44%). Conclusions: These gaps represent patient-centered priorities and targets for supportive interventions.
Mallotucin D, a Clerodane Diterpenoid from Croton crassifolius, Suppresses HepG2 Cell Growth via Inducing Autophagic Cell Death and Pyroptosis
Authors: Dai Xiaoyong , Sun Fen , Deng Kexin , Lin Gaoyang , Yin Wenjing , Chen Huaqing , Yang Dongye , Liu Kewei , Zhang Yubo , Huang Laiqiang
Published: November 2022

Hepatocellular carcinoma (HCC) is a major subtype of primary liver cancer with a high mortality rate. Pyroptosis and autophagy are crucial processes in the pathophysiology of HCC. Searching for efficient drugs targeting pyroptosis and autophagy with lower toxicity is useful for HCC treatment. Mallotucin D (MLD), a clerodane diterpenoid from Croton crassifolius, has not been previously reported for its anticancer effects in HCC. This study aims to evaluate the inhibitory effects of MLD in HCC and explore the underlying mechanism. We found that the cell proliferation, DNA synthesis, and colony formation of HepG2 cells and the angiogenesis of HUVECs were all greatly inhibited by MLD. MLD caused mitochondrial damage and decreased the TOM20 expression and mitochondrial membrane potential, inducing ROS overproduction. Moreover, MLD promoted the cytochrome C from mitochondria into cytoplasm, leading to cleavage of caspase-9 and caspase-3 inducing GSDMD-related pyroptosis. In addition, we revealed that MLD activated mitophagy by inhibiting the PI3K/AKT/mTOR pathway. Using the ROS-scavenging reagent NAC, the activation effects of MLD on pyroptosis- and autophagy-related pathways were all inhibited. In the HepG2 xenograft model, MLD effectively inhibited tumor growth without detectable toxicities in normal tissue. In conclusion, MLD could be developed as a candidate drug for HCC treatment by inducing mitophagy and pyroptosis via promoting mitochondrial-related ROS production.
Properties of Protein Isolates from Marine Hydrobionts Obtained by Isoelectric Solubilisation/Precipitation: Influence of Temperature and Processing Time
Authors: Derkach Svetlana R. , Kuchina Yuliya A. , Kolotova Daria S. , Petrova Ludmila A. , Volchenko Vasily I. , Glukharev Andrei Yu. , Grokhovsky Vladimir A.
Published: November 2022

Protein isolates were obtained from marine hydrobionts by the method of isoelectric precipitation with a preliminary stage of protein alkaline solubilisation. Northern blue whiting was chosen as the raw material. Various technological modes of the solubilisation stage were used: the temperature of the reaction mixture was 4 or 20 °C, and the duration was 4 or 16 h. The yield of the product was 44–45% with a high content of the main component (protein) equal to about 95%. It has been shown that a decrease in the temperature and duration of the alkaline solubilisation stage provides the production of protein isolates with good technological properties, a low solubility, high swelling and high emulsifying ability, necessary for its use in the production of functional food products, including therapeutic and prophylactic effects. These technological properties are explained by a change in the composition and structure of the protein, the change being an increase in the content of essential amino acids and the proportion of α-helices in the polypeptide chain. The main patterns obtained will be used to obtain protein isolates from marine molluscs.
Deglycosylation Increases the Aggregation and Angiogenic Properties of Mutant Tissue Inhibitor of Metalloproteinase 3 Protein: Implications for Sorsby Fundus Dystrophy
Authors: Qi Jian Hua , Anand-Apte Bela
Published: November 2022

Sorsby fundus dystrophy (SFD) is an autosomal dominant macular disorder caused by mutations in tissue Inhibitor of the metalloproteinase-3 (TIMP3) gene with the onset of symptoms including choroidal neovascularization as early as the second decade of life. We have previously reported that wild-type TIMP3 is an endogenous angiogenesis inhibitor that inhibits Vascular Endothelial Growth Factor (VEGF)-mediated signaling in endothelial cells. In contrast, SFD-related S179C-TIMP3 when expressed in endothelial cells, does not have angiogenesis-inhibitory properties. To evaluate if this is a common feature of TIMP3 mutants associated with SFD, we examined and compared endothelial cells expressing S179C, Y191C and S204C TIMP3 mutants for their angiogenesis-inhibitory function. Western blot analysis, zymography and reverse zymography and migration assays were utilized to evaluate TIMP3 protein, Matrix Metalloproteinase (MMP) and MMP inhibitory activity, VEGF signaling and in vitro migration in endothelial cells expressing (VEGF receptor-2 (VEGFR-2) and wild-type TIMP3 or mutant-TIMP3. We demonstrate that mutant S179C, Y191C- and S204C-TIMP3 all show increased glycosylation and multimerization/aggregation of the TIMP3 protein. In addition, endothelial cells expressing TIMP3 mutations show increased angiogenic activities and elevated VEGFR-2. Removal of N-glycosylation by mutation of Asn184, the only potential N-glycosylation site in mutant TIMP3, resulted in increased aggregation of TIMP3, further upregulation of VEGFR-2, VEGF-induced phosphorylation of VEGFR2 and VEGF-mediated migration concomitant with reduced MMP inhibitory activity. These results suggest that even though mutant TIMP3 proteins are more glycosylated, post-translational deglycosylation may play a critical role in the aggregation of mutant TIMP3 and contribute to the pathogenesis of SFD. The identification of factors that might contribute to changes in the glycome of patients with SFD will be useful. Future studies will evaluate whether variations in the glycosylation of mutant TIMP3 proteins are contributing to the severity of the disease.
Evaluation of Fusarium Head Blight Resistance Effects by Haplotype-Based Genome-Wide Association Study in Winter Wheat Lines Derived by Marker Backcrossing Approach
Authors: Radecka-Janusik Magdalena , Piechota Urszula , Piaskowska Dominika , Góral Tomasz , Czembor Paweł
Published: November 2022

Fusarium head blight (FHB) of wheat caused by Fusarium species is a destructive disease, causing grain yield and quality losses. Developing FHB-resistant cultivars is crucial to minimize the extent of the disease. The first objective of this study was incorporation of Fhb1 from a resistant donor into five Polish wheat breeding lines with good agronomical traits and different origins. We also performed a haplotype-based GWAS to identify chromosome regions in derived wheat families associated with Fusarium head blight resistance. As a result of marker-assisted backcrossing (MABC), five wheat combinations were obtained. Fungal inoculation and disease assessment were conducted for two years, 2019 and 2020. In 2019 the average phenotypic response of type II resistance was 2.2, whereas in 2020 it was 2.1. A haploblock-based GWAS performed on 10 phenotypic traits (related to type of resistance, year of experiment and FHB index) revealed nine marker–trait associations (MTA), among which six belong to chromosome 2D, two to 3B and one to 7D. Phenotypic variation (R2) explained by the identified haplotypes in haploblocks ranged from 6% to 49%. Additionally, an association weight matrix (AWM) was created, giving the partial correlation–information theory (PCIT) pipeline of 171 edges and 19 nodes. The resultant data and high level of explained phenotypic variance of MTA create the opportunity for data utilization in MAS.
Hormone Receptor Expression in Primary and Recurrent High-Grade Serous Ovarian Cancer and Its Implications in Early Maintenance Treatment
Authors: Vetter Marcus , Stadlmann Sylvia , Bischof Evelyne , Georgescu Margarint Elena Laura , Schötzau Andreas , Singer Gad , Heinzelmann-Schwarz Viola , Montavon Céline
Published: November 2022

Endocrine therapy is an effective treatment for low-grade serous ovarian cancer. However, the role of estrogen and progesterone receptors as biomarkers for high-grade serous ovarian cancer (HGSOC) is yet to be elucidated because not all estrogen and progesterone receptor-positive tumors benefit from anti-estrogen therapy. The degree of expression is presumed to play a vital role; however, that role is not well-defined in ovarian cancer. We aimed to determine the role of estrogen and progesterone receptor expression in primary and paired relapsed HGSOC. In this study, primary and matched relapsed tumor samples were collected from 80 patients with International Federation of Gynecology and Obstetrics Stage II–IV HGSOC. Tissue microarray was conducted and immunohistochemistry for estrogen and progesterone receptor expression was performed. Two independent pathologists performed the tissue microarray analysis with the Immunoreactive Score and Allred Total score. In the paired analysis, no significant difference in estrogen receptor expression was observed. However, progesterone receptor expression was significantly lower in patients with recurrent platinum-sensitive HGSOC. We conclude that anti-estrogen therapy targeting estrogen receptor positive HGSOC could be administered in primary and relapsed settings. The use of endocrine maintenance with an aromatase inhibitor in patients with estrogen receptor positive HGSOC needs to be further evaluated and validated in a randomized controlled trial.
Cold-Induced Physiological and Biochemical Alternations and Proteomic Insight into the Response of Saccharum spontaneum to Low Temperature
Authors: Zhang Bao-Qing , Huang Yu-Xin , Zhou Zhong-Feng , Zhou Shan , Duan Wei-Xing , Yang Cui-Fang , Gao Yi-Jing , Zhang Ge-Min , Song Xiu-Peng , Zhang Xiao-Qiu , Li Ao-Mei , Huang Dong-Liang , Li Yang-Rui
Published: November 2022

Sugarcane, a cash crop, is easily affected by low temperature, which results in a decrease in yield and sugar production. Breeding a new variety with cold tolerance is an essential strategy to reduce loss from cold stress. The identification of germplasms and genes/proteins with cold tolerance is a vital step in breeding sugarcane varieties with cold tolerance via a conventional program and molecular technology. In this study, the physiological and biochemical indices of 22 genotypes of S. spontaneum were measured, and the membership function analysis method was used to comprehensively evaluate the cold tolerance ability of these genotypes. The physiological and biochemical indices of these S. spontaneum genotypes showed a sophisticated response to low temperature. On the basis of the physiological and chemical indices, the genotypes were classified into different cold tolerance groups. Then, the high-tolerance genotype 1027 and the low-tolerance genotype 3217 were selected for DIA-based proteomic analysis by subjecting them to low temperature. From the four comparison groups, 1123, 1341, 751, and 1693 differentially abundant proteins (DAPs) were identified, respectively. The DAPs based on genotypes or treatments participated in distinct metabolic pathways. Through detailed analysis of the DAPs, some proteins related to protein homeostasis, carbohydrate and energy metabolism, amino acid transport and metabolism, signal transduction, and the cytoskeleton may be involved in sugarcane tolerance to cold stress. Furthermore, five important proteins related to cold tolerance were discovered for the first time in this study. This work not only provides the germplasms and candidate target proteins for breeding sugarcane varieties with cold tolerance via a conventional program and molecular breeding, but also helps to accelerate the determination of the molecular mechanism underlying cold tolerance in sugarcane.
Bacteriophage-Mediated Cancer Gene Therapy
Authors: Petrov Gleb , Dymova Maya , Richter Vladimir
Published: November 2022

Bacteriophages have long been considered only as infectious agents that affect bacterial hosts. However, recent studies provide compelling evidence that these viruses are able to successfully interact with eukaryotic cells at the levels of the binding, entry and expression of their own genes. Currently, bacteriophages are widely used in various areas of biotechnology and medicine, but the most intriguing of them is cancer therapy. There are increasing studies confirming the efficacy and safety of using phage-based vectors as a systemic delivery vehicle of therapeutic genes and drugs in cancer therapy. Engineered bacteriophages, as well as eukaryotic viruses, demonstrate a much greater efficiency of transgene delivery and expression in cancer cells compared to non-viral gene transfer methods. At the same time, phage-based vectors, in contrast to eukaryotic viruses-based vectors, have no natural tropism to mammalian cells and, as a result, provide more selective delivery of therapeutic cargos to target cells. Moreover, numerous data indicate the presence of more complex molecular mechanisms of interaction between bacteriophages and eukaryotic cells, the further study of which is necessary both for the development of gene therapy methods and for understanding the cancer nature. In this review, we summarize the key results of research into aspects of phage–eukaryotic cell interaction and, in particular, the use of phage-based vectors for highly selective and effective systemic cancer gene therapy.
The Role of Mitochondrial Enzymes, Succinate-Coupled Signaling Pathways and Mitochondrial Ultrastructure in the Formation of Urgent Adaptation to Acute Hypoxia in the Myocardium
Authors: Germanova Elita , Khmil Natalya , Pavlik Lyubov , Mikheeva Irina , Mironova Galina , Lukyanova Ludmila
Published: November 2022

The effect of a single one-hour exposure to three modes of hypobaric hypoxia (HBH) differed in the content of O2 in inhaled air (FiO2—14%, 10%, 8%) in the development of mitochondrial-dependent adaptive processes in the myocardium was studied in vivo. The following parameters have been examined: (a) an urgent reaction of catalytic subunits of mitochondrial enzymes (NDUFV2, SDHA, Cyt b, COX2, ATP5A) in the myocardium as an indicator of the state of the respiratory chain electron transport function; (b) an urgent activation of signaling pathways dependent on GPR91, HIF-1α and VEGF, allowing us to assess their role in the formation of urgent mechanisms of adaptation to hypoxia in the myocardium; (c) changes in the ultrastructure of three subpopulations of myocardial mitochondria under these conditions. The studies were conducted on two rat phenotypes: rats with low resistance (LR) and high resistance (HR) to hypoxia. The adaptive and compensatory role of the mitochondrial complex II (MC II) in maintaining the electron transport and energy function of the myocardium in a wide range of reduced O2 concentrations in the initial period of hypoxic exposure has been established. The features of urgent reciprocal regulatory interaction of NAD- and FAD-dependent oxidation pathways in myocardial mitochondria under these conditions have been revealed. The data indicating the participation of GPR91, HIF-1a and VEGF in this process have been obtained. The ultrastructure of the mitochondrial subpopulations in the myocardium of LR and HR rats differed in normoxic conditions and reacted differently to hypoxia of varying severity. The parameters studied together are highly informative indicators of the quality of cardiac activity and metabolic biomarkers of urgent adaptation in various hypoxic conditions.
Molecular Advances in SARS-CoV-2: A Brief Update on Transmission, Infection, and Pathology Aspects
Authors: Mesquita João R.
Published: November 2022

It all started back in late 2019 with a virus making a leap, crossing the barrier of species from an animal reservoir to a human and quickly spreading around the world [...]
Liver Steatosis and Steatohepatitis Alter Bile Acid Receptors in Brain and Induce Neuroinflammation: A Contribution of Circulating Bile Acids and Blood-Brain Barrier
Authors: Fiaschini Noemi , Mancuso Mariateresa , Tanori Mirella , Colantoni Eleonora , Vitali Roberta , Diretto Gianfranco , Lorenzo Rebenaque Laura , Stronati Laura , Negroni Anna
Published: November 2022

A tight relationship between gut-liver diseases and brain functions has recently emerged. Bile acid (BA) receptors, bacterial-derived molecules and the blood-brain barrier (BBB) play key roles in this association. This study was aimed to evaluate how non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) impact the BA receptors Farnesoid X receptor (FXR) and Takeda G-protein coupled receptor 5 (TGR5) expression in the brain and to correlate these effects with circulating BAs composition, BBB integrity and neuroinflammation. A mouse model of NAFLD was set up by a high-fat and sugar diet, and NASH was induced with the supplementation of dextran-sulfate-sodium (DSS) in drinking water. FXR, TGR5 and ionized calcium-binding adaptor molecule 1 (Iba-1) expression in the brain was detected by immunohistochemistry, while Zonula occludens (ZO)-1, Occludin and Plasmalemmal Vesicle Associated Protein-1 (PV-1) were analyzed by immunofluorescence. Biochemical analyses investigated serum BA composition, lipopolysaccharide-binding protein (LBP) and S100β protein (S100β) levels. Results showed a down-regulation of FXR in NASH and an up-regulation of TGR5 and Iba-1 in the cortex and hippocampus in both treated groups as compared to the control group. The BA composition was altered in the serum of both treated groups, and LBP and S100β were significantly augmented in NASH. ZO-1 and Occludin were attenuated in the brain capillary endothelial cells of both treated groups versus the control group. We demonstrated that NAFLD and NASH provoke different grades of brain dysfunction, which are characterized by the altered expression of BA receptors, FXR and TGR5, and activation of microglia. These effects are somewhat promoted by a modification of circulating BAs composition and by an increase in LBP that concur to damage BBB, thus favoring neuroinflammation.