bluuebunny/crossref_metadata_2025 huggingface dataset:
Non-use and inadequate use of cervical cancer screening among a representative sample of women in the United States
Authors: Narcisse Marie-Rachelle , McElfish Pearl A. , Hallgren Emily , Pierre-Joseph Natalie , Felix Holly C.
Published: April 2024

Introduction

Women's adherence to the United States (U.S.) Preventive Services Task Force guidelines for cervical cancer screening was determined by examining predisposing, enabling, and needs factors from Andersen's Behavioral Model of Health Services Use conceptual framework.

Methods

The outcome was operationalized as cervical cancer screening use, non-use, and inadequate-use. Multinomial logistic regression was conducted on data from the 2019 National Health Interview Survey of 7,331 eligible women aged 21–65.

Results

Compared with women who used cervical cancer screening services, women aged 30–65 were less likely to be Non-Users than those aged 21–29. Hispanic, Asian, and American Indian/Alaska Native (AIAN) women were more likely to be Non-Users than White women. More educated women were less likely to be Non-Users. Foreign-born women <10 years in the U.S. were more likely to be Non-Users than U.S.-born women. Women with financial hardship were less likely to be Non-Users. Poorer women and uninsured women were more likely to be Non-Users. Women with children in their household were less likely to be Non-Users than those without children. Women who had a well-visit in the past year were less likely to be Non-Users. Women with a history of human papillomavirus (HPV) vaccination were less likely to be Non-Users. Compared with women who used cervical cancer screening services, women aged 30–65 were less likely to be Inadequate-Users. AIAN women were more likely to be Inadequate-Users. Women of other races were less likely to be Inadequate-Users. Employed women were less likely to be Inadequate-Users. Uninsured women were more likely to be Inadequate-Users. Women who had a well-visit within a year were less likely to be Inadequate-Users. Women with past HPV vaccination were more likely to be Inadequate-Users. Smokers were less likely to be Inadequate-Users.

Discussion

Predisposing, enabling, and needs factors are differently associated with non-use and inadequate use of cervical cancer screening. Understanding factors associated with the use, non-use, and inadequate use of cervical cancer screening is crucial to avoid or curb unnecessary tests, increased costs to both society and individuals, and the ill-allocation of limited resources.
In-situ optical water quality monitoring sensors—applications, challenges, and future opportunities
Authors: Kumar Manish , Khamis Kieran , Stevens Rob , Hannah David M. , Bradley Chris
Published: April 2024

Water quality issues remain a major cause of global water insecurity, and real-time low-cost monitoring solutions are central to the remediation and management of water pollution. Optical sensors, based on fluorescence, absorbance, scattering and reflectance-based principles, provide effective water quality monitoring (WQM) solutions. However, substantial challenges remain to their wider adoption across scales and environments amid cost and calibration-related concerns. This review discusses the current and future challenges in optical water quality monitoring based on multi-peak fluorescence, full-spectrum absorbance, light-scattering and remotely sensed surface reflectance. We highlight that fluorescence-based sensors can detect relatively low concentrations of aromatic compounds (e.g., proteins and humic acids) and quantify and trace organic pollution (e.g., sewage or industrial effluents). Conversely, absorbance-based sensors (Ultraviolet-Visible-Infra-red, UV-VIS-IR) are suitable for monitoring a wider range of physiochemical variables (e.g., nitrate, dissolved organic carbon and turbidity). Despite being accurate under optimal conditions, measuring fluorescence and absorbance can be demanding in dynamic environments due to ambient temperature and turbidity effects. Scattering-based turbidity sensors provide a detailed understanding of sediment transport and, in conjunction, improve the accuracy of fluorescence and absorbance measurements. Recent advances in micro-sensing components such as mini-spectrometers and light emitting diodes (LEDs), and deep computing provide exciting prospects of in-situ full-spectrum analysis of fluorescence (excitation-emission matrices) and absorbance for improved understanding of interferants to reduce the signal-to-noise ratio, improve detection accuracies of existing pollutants, and enable detection of newer contaminants. We examine the applications combining in-situ spectroscopy and remotely sensed reflectance for scaling Optical WQM in large rivers, lakes and marine bodies to scale from point observations to large water bodies and monitor algal blooms, sediment load, water temperature and oil spills. Lastly, we provide an overview of future applications of optical techniques in detecting emerging contaminants in treated and natural waters. We advocate for greater synergy between industry, academia and public policy for effective pollution control and water management.
Natural deep eutectic liquid and ultrasound-assisted extraction of milk thistle phenolics and their hepatoprotective activities
Authors: Nasrullah Nabiha , Akram Sumia , Rashid Ayoub , Adnan Ahmad , Mushtaq Muhammad
Published: April 2024

Introduction

The present research describes the utilization of natural deep eutectic solvent in combination with ultrasound for the extraction of milk thistle polyphenols. The extracts obtained under different conditions were evaluated for theirin vitroantioxidant activities and hepatoprotective activities in Albino mice (in vivo).

Methods

The extraction parameters involving liquid-to-solid ratio (S/L), ethanol-to-natural deep eutectic solvent ratio (EtOH/DES), extraction time (t), and ultrasound treatment time were investigated and optimized to enhance the recovery of bioactives, their phenolic content, Trolox equivalent antioxidant capacity (TEAC), and radical scavenging capacity (RSC).

Results and discussions

The extraction of milk thistle polyphenols using glucose/citric acid (1:1)-based natural deep eutectic liquid coupled with ultrasound for 1.0 min under an S/L of 6.2, EtOH/DES 9, and extraction time of 120.8 min offered 35.89 ± 2.29 g/100 g of crude extracts, which were three-fold higher than that by conventional solvent extraction (CSE). Each gram of milk thistle extracts thus obtained comprised 377.93 ± 6.17 mg GAE of TPC and exhibited 298.70 ± 3.06 μmol TEAC and 93.16 ± 0.58 percent inhibition of DPPH free radicals. In addition, the administration of milk thistle extracts obtained as given above at 25 mg/Kg body weight in Albino mice significantly (p≤ 0.05) improved the liver function parameters at the end of treatment (7 days).

Conclusion

Overall, it was observed that NaDES in combination with medium-level ultrasound energy (700 watt) might work as a sustainable choice to enhance the recovery of bioactive phenolics from milk thistle powder without compromising their antioxidant and hepatoprotective potential.
A case report of parotid gland epithelioid hemangioendothelioma
Authors: Wang Ke , Hu Jianhong , Wen Jiazhu , Zhou Shuxia , Ye Linfeng , Fang Chun , Guan Jiacheng , Luo Xiao
Published: April 2024

Epithelioid hemangioendothelioma (EHE) is a rare low-grade malignant vascular tumor. It mainly occurs in the liver, lungs, bones, and other parts of the body. Reports of epithelioid hemangioendothelioma in the parotid gland are rare in both domestic and international literature. Here, we present a case report of a parotid gland epithelioid hemangioendothelioma, including its complete clinical course and imaging findings, to improve the diagnosis and treatment of this unusual disease.

Case presentation

The patient, a 75-year-old female, presented with a swelling around the right ear for 2 months and pain for 20 days. Enhanced MRI of the parotid gland revealed a well-defined, round mass with homogeneous signal intensity. The mass showed low signal intensity on T1-weighted imaging, high signal intensity on T2-weighted imaging, nodular low signal intensity within, significant high signal intensity on DWI sequence, low signal intensity on ADC sequence, and heterogeneous enhancement in the arterial phase after intravenous injection of Gd-DTPA. Nodular non-enhancing low signal intensity was observed internally, and slight clearance was seen in the venous phase. The initial diagnosis before surgery was a benign lesion, but after histopathological and immunohistochemical examination, it was confirmed as epithelioid hemangioendothelioma.

Intervention

Complete tumor resection was performed.

Results

The patient experienced a favorable recovery, with meticulous follow-up conducted for up to 1 year revealing no signs of recurrence or metastasis. Continued patient surveillance is ongoing to substantiate and validate the long-term efficacy of the treatment.

Conclusion

Due to the extreme rarity of parotid gland epithelioid hemangioendothelioma, it often leads to a high misdiagnosis rate. The most common misdiagnosis is salivary gland lymphoma, followed by epithelioid hemangiosarcoma. When the lesion is multifocal, fusiform, with internal necrosis, and shows punctate low signal intensity on T2-weighted imaging, significant enhancement in the arterial phase, particularly with more pronounced peripheral enhancement, and persistent enhancement in the venous and delayed phases, epithelioid hemangioendothelioma should be considered. However, the current clinical diagnosis of epithelioid hemangioendothelioma still primarily relies on immunohistochemical methods.
Effects of adding bile acids to dietary storage japonica brown rice on growth performance, meat quality, and intestinal microbiota of growing–finishing Min pigs
Authors: Wang Chuanqi , Zheng Kexin , Wang Dali , Yu Hao , Zhao Yun , Fang Hengtong , Zhang Jing
Published: April 2024

Introduction

This study investigated the effects of storage japonica brown rice (SJBR) and bile acids (BA) on the growth performance, meat quality, and intestinal microbiota of growing–finishing Min pigs.

Methods

A total of 24 healthy Min pigs with a similar body weight of 42.25 ± 2.13 kg were randomly divided into three groups with eight replicates of one pig each. The groups were as follows: CON (50% corn), SJBR (25% corn +25% SJBR), and SJBR + BA (25% corn +25% SJBR +0.025% hyodeoxycholic acid). The experimental period lasted from day 90 (the end of the nursery phase) to day 210 (the end of the finishing phase).

Results

The results showed the following: (1) Compared with the CON group, there was no significant difference in the average daily gain (ADG) and average daily feed intake (ADFI) of the SJBR and SJBR + BA groups, and the feed conversion ratio (FCR) was significantly decreased (p < 0.05). (2) Compared with the CON group, the total protein (TP) content in the serum was significantly increased, and the blood urea nitrogen (BUN) content was significantly decreased (p < 0.05) in the SJBR and SJBR + BA groups; moreover, HDL-C was significantly higher by 35% (p < 0.05) in the SJBR + BA group. (3) There were no significant differences in carcass weight, carcass length, pH, drip loss, cooking loss, and shear force among the groups; the eye muscle area was significantly increased in the SJBR group compared with the CON group (p < 0.05); back fat thickness was significantly decreased in the SJBR + BA group compared with the SJBR group (p < 0.05); and the addition of SJBR significantly increased the mRNA expression of MyHC I in the longissimus dorsi (LD) muscle of growing–finishing Min pigs (p < 0.05). (4) The cecal bacteria were detected using 16S rDNA, and the proportion of Lactobacillus was increased gradually at the genus level, but there was no significant difference among the different groups.

Conclusion

In conclusion, 25% SJBR can improve the growth performance and increase the abundance of intestinal beneficial bacteria, and based on this, adding bile acids can reduce the back fat thickness of growing–finishing Min pigs.
Certified Fellowship in Abdominal Wall Surgery—A Collaboration Between the UEMS and the European Hernia Society
Authors: Köckerling Ferdinand , Morales-Conde Salvador , Simons Maarten , Casanova Daniel , East Barbora , de Beaux Andrew , Henriksen Nadia , Roka Sebastian , Felice Arthur
Published: April 2024

Background: Abdominal wall surgery (AWS) is characterised by the increasing caseload and the complexity of the surgical procedures. The introduction of a tailored approach to AWS utilising laparoendoscopic, robotic and/or open techniques requires the surgeon to master several surgical techniques. All of which have an associated learning curve, and the necessary knowledge/experience to know which operation is the right one for the individual patient. However, the reality in general surgery training shows that training in just a limited number of procedures is not enough. By the end of general surgery training, many chief residents do not feel they are yet ready to carry out surgery independently. Therefore, hernia surgery experts and societies have called for the introduction of a Fellowship in Abdominal Wall Surgery.

Methods: The UEMS (Union Européenne des Médecins Spécialistes, European Union of Medical Specialists) in collaboration with the European Hernia Society (EHS) introduced a fellowship by examination in 2019. As a prerequisite, candidates must complete further training of at least 2 years with a special focus on abdominal wall surgery after having completed their training in general surgery. To be eligible for the examination, candidates must provide evidence of having performed 300 hernia procedures. In addition, candidates must have accrued sufficient “knowledge points” by attending abdominal wall surgery congresses, courses and clinical visitations, and engaged in scientific activities. On meeting the requirements, a candidate may be admitted to the written and oral examination.

Results: To date, three examinations have been held on the occasion of the Annual Congress of the European Hernia Society in Copenhagen (2021), Manchester (2022) and Barcelona (2023). Having met the requirements, 48 surgeons passed the written and oral examination and were awarded the Fellow European Board of Surgery—Abdominal Wall Surgery certificate. During this time period, a further 25 surgeons applied to sit the examination but did not fulfil all the criteria to be eligible for the examination. Fifty experienced abdominal wall surgeons applied to become an Honorary Fellow European Board of Surgery—Abdominal Wall Surgery. Fourty eight were successful in their application.

Conclusion: The Fellowship of the European Board of Surgery - Abdominal Wall Surgery by examination has been successfully introduced at European level by the joint work of the UEMS and the EHS. The examination is also open to surgeons who work outside the European area, if they can fulfil the eligibility criteria.
The Impact of Green Human Resource Management Practices on Employees, Clients, and Organizational Performance: A Literature Review
Authors: AlKetbi Aisha , Rice John
Published: April 2024

This literature review aims to examine the relationship between Green Human Resource Management (G-HRM) practices and various outcomes, including employee green attitudes, employee green satisfaction, client green satisfaction, employee green behavior, and organizational green performance. We reviewed existing literature on G-HRM practices and their impact on the selected outcomes. The review process involved the identification of articles through a systematic search in Scopus and Web of Science databases from January 2013 to December 2023. The search retrieved 2142 citations; of them, a total of 17 articles were deemed eligible for this review. The reviewed literature provides good evidence supporting a positive predictive relationship between G-HRM practices and employee green attitudes, employee green satisfaction, client green satisfaction, employee green behavior, and organizational green performance. However, there is a notable gap in studies exploring the influence of G-HRM practices on employee and client satisfaction. Overall, G-HRM practices emerge as a crucial tool for fostering environmentally conscious attitudes and behaviors among employees, ultimately contributing to enhanced employee satisfaction and improved organizational ecological performance. Future research should pay attention to the mechanisms underlying these relationships and explore potential moderating factors to enrich our understanding of the interrelated dynamics between G-HRM practices and sustainable outcomes.
Scrutinizing Business Development Research: Dynamic Retrospective Analysis and Conceptual Evolution
Authors: Wei Yu-Min , Lin Hsin-Mei
Published: April 2024

While prevalent in enterprise practice, business development (BD) activities have garnered limited attention within academic circles, leading to a noticeable dearth of discussions regarding their comprehensive understanding and the contemporary research landscape. Previous research has underscored the pivotal role and importance of business development activities in generating new growth opportunities and enhancing dynamic capabilities for enterprises. This article seeks to bridge this academic gap by synthesizing six decades of research literature on enterprise business development activities and tracing the progression of its research trajectory to uncover its complete narrative. Its contributions are manifold: firstly, we intensified the foundational elements of business development research by conducting a meticulous examination of various perspectives and providing insights into former ambiguities surrounding crucial aspects; secondly, it elucidates how contextual factors influence the emergence and evolution of enterprises’ business development activities, highlighting its research significance across different eras; thirdly, it conducts a comprehensive exploration of various research streams within business development and their nuanced interactions across key dimensions. Through these endeavors, this study aims to enrich academic discourse in the domain of business development, establishing a robust academic foundation for subsequent research.
Dynamic Energy Use Efficiency, Carbon Input, and Agricultural Benefits of Multiple Cropping in Southern China—A Case Study from Guangdong Province
Authors: Zhang Tantan , Deng Siying , Li Yanhong , Qing Bowen , Li Wu , Mo Zhaowen
Published: April 2024

Background: With the purpose of exploring the development of new quality productive forces in Guangdong, the present study hypothesized that reducing energy and carbon inputs was beneficial for increasing Guangdong’s multi-cropping agricultural energy output and economic returns. Methods: The energy use efficiency of crop production and the corresponding carbon input and agricultural benefit trends from 2011 to 2021 were examined by linear regression analysis for Guangdong Province, China. The corresponding development trends were also predicted using a grey model. Results: The results indicated that the total energy output increased by 12.50%, wherein the energy productivity levels of peanuts, vegetables, mulberry, and fruits increased greatly (51.27–106.17%), while the total energy input and the total carbon input decreased by 12.17% and 6.37%, respectively. Moreover, the energy input per carbon input decreased by 6.19%, while the energy output per carbon input increased by 20.15%. Both energy-related indicators and economic-related indicators all had substantially increased (28.08–44.97% and 83.86–120.91%, respectively). Grey model predictions show that the agricultural output value increased steadily under the current agricultural policy of reducing fossil energy input. Conclusions: The current low-carbon and high-output agricultural model is beneficial for increasing Guangdong’s multi-cropping agricultural economic returns and mitigating greenhouse effects.
Climatic Conditions Influence the Nutritive Value of Wheat as a Feedstuff for Broiler Chickens
Authors: Khoddami Ali , Tan Daniel K. Y. , Messina Valeria , Chrystal Peter V. , Thistlethwaite Rebecca , Caldwell Robert A. , Trethowan Richard , Toghyani Mehdi , Macelline Shemil , Bai Yunlong , Selle Peter H. , Liu Sonia Y.
Published: April 2024

Forty wheat samples of ten wheat varieties harvested from optimal or late sowings in 2019 and 2020 were evaluated for nutrient composition. This included crude protein (CP), starch, amino acids, minerals, phytate-phosphorus (phytate-P) and non-starch polysaccharides (NSPs). The objective was to investigate the impact of high temperature on wheat grain quality as a feedstuff for broiler chickens. Growth performance and economic impact of such changes were predicted by the Emmans, Fisher and Gous broiler growth model. On average, 2019 was 1 °C hotter than 2020 during the growing season (Narrabri, NSW 2390, Australia). The wheat harvested in 2019 had higher concentrations of CP, phytate-P, total P and calcium. In 2019, late sowing increased average protein concentrations from 166.6 to 190.2 g/kg, decreased starch concentration from 726 to 708 g/kg and increased total NSPs from 693 to 73.9 g/kg. Unlike the 2019 harvest, the late sowing in 2020 had no impact on CP concentrations in almost all wheat varieties. The 2019 varieties had higher concentrations of 16 assessed amino acids (p < 0.001) compared to the 2020 harvest. The largest difference was in lysine (19.2%), and the smallest difference was in proline (11.1%). It was predicted that broiler diets formulated from 2019 wheat varieties would have better efficiency of feed conversion with an advantage of 2.53% (1.539 versus 1.579) than 2020 varieties to 35 days post-hatch. This would translate to a cost saving of approximately AUD 16.45 per tonne of feed, much of which would represent additional profit.
Arbuscular Mycorrhizal Fungi, Ascophyllum nodosum, Trichoderma harzianum, and Their Combinations Influence the Phyllochron, Phenology, and Fruit Quality of Strawberry Plants
Authors: Chiomento José Luís Trevizan , Fracaro Júlia , Görgen Manuela , Fante Rudinei , Dal Pizzol Emanuele , Welter Matheus , Klein Arthur Pegoraro , Trentin Thomas dos Santos , Suzana-Milan Crislaine Sartori , Palencia Pedro
Published: April 2024

One biostrategy to boost the sustainability of strawberry cultivation is the application of biostimulants to the growing substrate. Here, we investigated whether the use of biostimulants and their combinations affects the strawberry plants’ phyllochron, phenology, and fruit quality. We tested the absence (control) and presence of biostimulants (arbuscular mycorrhizal fungi (AMF), Ascophyllum nodosum (AN), Trichoderma harzianum (TH), AMF + AN, AMF + TH, AN + TH, and AMF + AN + TH). The experimental design used was in completely randomized blocks (four replications). AMF was represented by a multi-species on-farm inoculant; A. nodosum was represented by the commercial product Acadian®; and T. harzianum was represented by the commercial product Trichodermil®. The leaf emission rate, the occurrence and duration of phenological stages, and fruit quality were assessed. The greatest precocity in terms of harvesting the first fruit was observed in plants grown with AMF + TH, which also had the lowest phyllochron (77.52 °C day·leaf−1). Those treated only with AMF were the latest (144.93 °C day·leaf−1). More flavorful fruits were produced by plants grown with AMF + TH. Plants inoculated with the AMF community, whether or not associated with A. nodosum and T. harzianum, had more than 94% mycorrhizal colonization. We conclude that AMF, A. nodosum, T. harzianum, and their combinations influence the phenology, phyllochron, and fruit quality of strawberry plants. In the growing conditions of the Brazilian subtropics, the AMF and T. harzianum combination shortens the strawberry cycle, from transplanting the daughter plants to harvesting the first fruit, and improves the fruit flavor.
Assessment of Yield and Quality of Eggplant (Solanum melongena L.) Fruits Improved by Biodegradable Mulching Film in Two Different Regions of Southern Italy
Authors: Di Miceli Giuseppe , Iacuzzi Nicolò , Leto Claudio , Cozzolino Eugenio , Di Mola Ida , Ottaiano Lucia , Mori Mauro , Bella Salvatore La
Published: April 2024

Low-density polyethylene (LDPE) mulching films have an important function in crop cultivation; at the end of their life, however, their removal and disposal become both an economic and environmental problem. One possible alternative to low-density polyethylene (LDPE) mulch is provided by certified soil-biodegradable mulch films, such as those produced by Novamont and commercially available under the trade name MaterBi®. MaterBi is a biodegradable thermoplastic material made with starch and a biodegradable copolyester based on proprietary technology. In this study, we compared two biodegradable MaterBi®-based films (commercial and experimental films) with bare soil and a low-density polyethylene to evaluate their effect on yield and on a number of qualitative characteristics (organoleptic and nutraceutical composition) of eggplant fruits (cv Mirabelle F1) grown in two different regions in Southern Italy (Sicily and Campania). In our study, the use of biodegradable MaterBi® films improved not only yield and production parameters, such as the number and average weight of fruits, but also lipophilic and hydrophilic antioxidant activity and phenolic and ascorbic acid content. For many parameters, responses differed according to the cultivation environment and, in particular, the site’s pedoclimatic conditions. Our results suggest that biodegradable MaterBi®-based mulching films are a potentially valid alternative to traditional LDPEs, providing the production and quality benefits reported above and promoting environmental sustainability, thanks to their positive biodegradable properties.
The Supplementation of Berberine in High-Carbohydrate Diets Improves Glucose Metabolism of Tilapia (Oreochromis niloticus) via Transcriptome, Bile Acid Synthesis Gene Expression and Intestinal Flora
Authors: Liu Hongyu , Wei Menglin , Tan Beiping , Dong Xiaohui , Xie Shiwei
Published: April 2024

Berberine is an alkaloid used to treat diabetes. This experiment aimed to investigate the effects of berberine supplementation in high-carbohydrate diets on the growth performance, glucose metabolism, bile acid synthesis, liver transcriptome, and intestinal flora of Nile tilapia. The six dietary groups were the C group with 29% carbohydrate, the H group with 44% carbohydrate, and the HB1-HB4 groups supplemented with 25, 50, 75, and 100 mg/kg of berberine in group H. The results of the 8-week trial showed that compared to group C, the abundance of Bacteroidetes was increased in group HB2 (p < 0.05). The cholesterol-7α-hydroxylase (CYP7A1) and sterol-27-hydroxylase (CYP27A1) activities were decreased and the expression of FXR was increased in group HB4 (p < 0.05). The pyruvate carboxylase (PC) and phosphoenolpyruvate carboxykinase (PEPCK) activities was decreased in group HB4 (p < 0.05). The liver transcriptome suggests that berberine affects carbohydrate metabolic pathways and primary bile acid synthesis pathways. In summary, berberine affects the glucose metabolism in tilapia by altering the intestinal flora structure, enriching differentially expressed genes (DEGs) in the bile acid pathway to stimulate bile acid production so that it promotes glycolysis and inhibits gluconeogenesis. Therefore, 100 mg/kg of berberine supplementation in high-carbohydrate diets is beneficial to tilapia.
Pulmonary and Gastrointestinal Parasitic Infections in Small Ruminant Autochthonous Breeds from Centre Region of Portugal—A Cross Sectional Study
Authors: Pereira Maria , Vila-Viçosa Maria , Coelho Catarina , Santos Carla , Esteves Fernando , Cruz Rita , Gomes Liliana , Henriques Diogo , Vala Helena , Nóbrega Carmen , Mega Ana , Melo Carolina , Malva Madalena , Braguez Joana , Mateus Teresa
Published: April 2024

The production of small ruminant autochthonous breeds in the Centre region of Portugal is practiced in a semi-extensive husbandry system, exposing animals to parasitic infections. The main objective of this study was to estimate the prevalence of lungworm infection and identify risk factors. Fecal samples of 203 goats and 208 sheep from 30 herds were collected per rectum and subjected to the modified Baermann test. The overall prevalence of infection was 57.7%, significantly higher in goats (95.6%) than in sheep (20.7%) (p < 0.001). According to the binary logistic regression model, sheep dewormed with albendazole, mebendazole plus closantel, or ivermectin plus clorsulon presented a risk of Protostrongylidae infection 29.702, 7.426, or 8.720 times higher, respectively, than those dewormed with eprinomectin. Additionally, the presence of gastrointestinal parasites was investigated in 307 fecal samples using Mini-FLOTAC®. The overall prevalence of infection was 86.3%, also significantly higher in goats (93.2%) than in sheep (79.9%) (p < 0.001). Strongyle-type eggs were the most frequently identified, both in sheep (69.8%) and goats (87.8%), followed by Eimeria oocysts (40.3% in sheep and 68.9% in goats). Considering the high prevalence and the burden of lungworm parasitic infection, it is urgent to determine its economic impact and the repercussions in animal health in the Centre region of Portugal to establish appropriate therapeutic guidelines.
Cross-Reactivity of N6AMT1 Antibodies with Aurora Kinase A: An Example of Antibody-Specific Non-Specificity
Authors: Brūmele Baiba , Serova Evgeniia , Lupp Aleksandra , Suija Mihkel , Mutso Margit , Kurg Reet
Published: April 2024

Primary antibodies are one of the main tools used in molecular biology research. However, the often-occurring cross-reactivity of primary antibodies complicates accurate data analysis. Our results show that three commercial polyclonal antibodies raised against N-6 adenine-specific DNA methyltransferase 1 (N6AMT1) strongly cross-react with endogenous and recombinant mitosis-associated protein Aurora kinase A (AURKA). The cross-reactivity was verified through immunofluorescence, immunoblot, and immunoprecipitation assays combined with mass spectrometry. N6AMT1 and AURKA are evolutionarily conserved proteins that are vital for cellular processes. Both proteins share the motif ENNPEE, which is unique to only these two proteins. We suggest that N6AMT1 antibodies recognise this motif in N6AMT1 and AURKA proteins and exhibit an example of “specific” non-specificity. This serves as an example of the importance of controls and critical data interpretation in molecular biology research.
Rabbits as a Reservoir of Multidrug-Resistant Escherichia coli: Clonal Lineages and Public Health Impact
Authors: Silva Adriana , Silva Vanessa , Tavares Teresa , López María , Rojo-Bezares Beatriz , Pereira José Eduardo , Falco Virgílio , Valentão Patrícia , Igrejas Gilberto , Sáenz Yolanda , Poeta Patrícia
Published: April 2024

Escherichia coli, including extended-spectrum β-lactamases (ESBL)-producing strains, poses a global health threat due to multidrug resistance, compromising food safety and environmental integrity. In industrial settings, rabbits raised for meat have the highest consumption of antimicrobial agents compared to other food-producing animals. The European Union is facing challenges in rabbit farming as rabbit consumption declines and antibiotic-resistant strains of E. coli cause enteric diseases. The aim of this study was to investigate the antibiotic resistance profile, genetic diversity, and biofilm formation in cefotaxime-resistant E. coli strains isolated from twenty rabbit farms in Northern Portugal to address the effect of the pressing issue of antibiotic resistance in the rabbit farming industry. Resistance to critically antibiotics was observed, with high levels of resistance to several categories, such as tetracycline, ampicillin, aztreonam, and streptomycin. However, all isolates were susceptible to cefoxitin and imipenem. Multidrug resistance was common, with strains showing resistance to all antibiotics tested. The blaCTX-M variants (blaCTX-3G and blaCTX-M9), followed by the tetracycline resistance genes, were the most frequent resistance genes found. ST10 clones exhibiting significant resistance to various categories of antibiotics and harboring different resistance genes were detected. ST457 and ST2325 were important sequence types due to their association with ESBL-E. coli isolates and have been widely distributed in a variety of environments and host species. The strains evaluated showed a high capacity for biofilm formation, which varied when they were grouped by the number of classes of antibiotics to which they showed resistance (i.e., seven different classes of antibiotics, six classes of antibiotics, and three/four/five classes of antibiotics). The One Health approach integrates efforts to combat antimicrobial resistance in rabbit farming through interdisciplinary collaboration of human, animal, and environmental health. Our findings are worrisome and raise concerns. The extensive usage of antibiotics in rabbit farming emphasizes the urgent need to establish active surveillance systems.
The Epidemiology, Management and Therapeutic Outcomes of Subdural Empyema in Neonates with Acute Bacterial Meningitis
Authors: Lee Wei-Ju , Tsai Ming-Horng , Hsu Jen-Fu , Chu Shih-Ming , Chen Chih-Chen , Yang Peng-Hong , Huang Hsuan-Rong , Chi Miao-Ching , Lee Chiang-Wen , Ou-Yang Mei-Chen
Published: April 2024

Background: Subdural empyema is one of the more serious complications of bacterial meningitis and therapeutic challenges to clinicians. We aimed to evaluate the clinical characteristics, treatment, and outcome of subdural empyema in neonates with bacterial meningitis. Methods: A retrospective cohort study was conducted in two medical centers in Taiwan that enrolled all cases of neonates with subdural empyema after bacterial meningitis between 2003 and 2020. Results: Subdural empyema was diagnosed in 27 of 153 (17.6%) neonates with acute bacterial meningitis compared with cases of meningitis without subdural empyema. The demographics and pathogen distributions were comparable between the study group and the controls, but neonates with subdural empyema were significantly more likely to have clinical manifestations of fever (85.2%) and seizure (81.5%) (both p values < 0.05). The cerebrospinal fluid results of neonates with subdural empyema showed significantly higher white blood cell counts, lower glucose levels and higher protein levels (p = 0.011, 0.003 and 0.006, respectively). Neonates with subdural empyema had a significantly higher rate of neurological complications, especially subdural effusions and periventricular leukomalacia. Although the final mortality rate was not increased in neonates with subdural empyema when compared with the controls, they were often treated much longer and had a high rate of long-term neurological sequelae. Conclusions: Subdural empyema is not uncommon in neonates with acute bacterial meningitis and was associated with a high risk of neurological complications, although it does not significantly increase the final mortality rate. Close monitoring of the occurrence of subdural empyema is required, and appropriate long-term antibiotic treatment after surgical intervention may lead to optimized outcomes.
Uncovering the Cardioprotective Potential of Diacerein in Doxorubicin Cardiotoxicity: Mitigating Ferritinophagy-Mediated Ferroptosis via Upregulating NRF2/SLC7A11/GPX4 Axis
Authors: El-Gohary Rehab M. , Okasha Asmaa H. , Abd El-Azeem Alaa H. , Abdel Ghafar Muhammad T. , Ibrahim Sarah , Hegab Islam I. , Farghal Eman E. , Shalaby Soha Abdel Fattah , Elshora Ola A. , ElMehy Aisha E. , Barakat Amany Nagy , Amer Basma Saed , Sobeeh Fatma G. , AboEl-Magd Gehan H. , Ghalwash Asmaa A.
Published: April 2024

Doxorubicin (DOX)-induced cardiotoxicity (DIC) is a life-threatening clinical issue with limited preventive approaches, posing a substantial challenge to cancer survivors. The anthraquinone diacerein (DCN) exhibits significant anti-inflammatory, anti-proliferative, and antioxidant actions. Its beneficial effects on DIC have yet to be clarified. Therefore, this study investigated DCN’s cardioprotective potency and its conceivable molecular targets against DIC. Twenty-eight Wister rats were assigned to CON, DOX, DCN-L/DOX, and DCN-H/DOX groups. Serum cardiac damage indices, iron assay, oxidative stress, inflammation, endoplasmic reticulum (ER) stress, apoptosis, ferritinophagy, and ferroptosis-related biomarkers were estimated. Nuclear factor E2-related factor 2 (NRF2) DNA-binding activity and phospho-p53 immunoreactivity were assessed. DCN administration effectively ameliorated DOX-induced cardiac cytomorphological abnormalities. Additionally, DCN profoundly combated the DOX-induced labile iron pool expansion alongside its consequent lethal lipid peroxide overproduction, whereas it counteracted ferritinophagy and enhanced iron storage. Indeed, DCN valuably reinforced the cardiomyocytes’ resistance to ferroptosis, mainly by restoring the NRF2/solute carrier family 7 member 11 (SLC7A11)/glutathione peroxidase 4 (GPX4) signaling axis. Furthermore, DCN abrogated the cardiac oxidative damage, inflammatory response, ER stress, and cardiomyocyte apoptosis elicited by DOX. In conclusion, for the first time, our findings validated DCN’s cardioprotective potency against DIC based on its antioxidant, anti-inflammatory, anti-ferroptotic, and anti-apoptotic imprint, chiefly mediated by the NRF2/SLC7A11/GPX4 axis. Accordingly, DCN could represent a promising therapeutic avenue for patients under DOX-dependent chemotherapy.
Cord Blood Adductomics Reveals Oxidative Stress Exposure Pathways of Bronchopulmonary Dysplasia
Authors: Lin Erika T. , Bae Yeunook , Birkett Robert , Sharma Abhineet M. , Zhang Runze , Fisch Kathleen M. , Funk William , Mestan Karen K.
Published: April 2024

Fetal and neonatal exposures to perinatal oxidative stress (OS) are key mediators of bronchopulmonary dysplasia (BPD). To characterize these exposures, adductomics is an exposure science approach that captures electrophilic addition products (adducts) in blood protein. Adducts are bound to the nucleophilic cysteine loci of human serum albumin (HSA), which has a prolonged half-life. We conducted targeted and untargeted adductomics to test the hypothesis that adducts of OS vary with BPD. We studied 205 preterm infants (≤28 weeks) and 51 full-term infants from an ongoing birth cohort. Infant plasma was collected at birth (cord blood), 1-week, 1-month, and 36-weeks postmenstrual age. HSA was isolated from plasma, trypsin digested, and analyzed using high-performance liquid chromatography–mass spectrometry to quantify previously annotated (known) and unknown adducts. We identified 105 adducts in cord and postnatal blood. A total of 51 known adducts (small thiols, direct oxidation products, and reactive aldehydes) were increased with BPD. Postnatally, serial concentrations of several known OS adducts correlated directly with supplemental oxygen exposure. The application of large-scale adductomics elucidated OS-mediated pathways of BPD. This is the first study to investigate the “neonatal–perinatal exposome” and to identify oxidative stress-related exposure biomarkers that may inform antioxidant strategies to protect the health of future generations of infants.
Litchi Pericarp Extract Treats Type 2 Diabetes Mellitus by Regulating Oxidative Stress, Inflammatory Response, and Energy Metabolism
Authors: Yang Ziming , Zhang Li , Liu Jinlei , Li Dianpeng
Published: April 2024

Litchi pericarp is rich in polyphenols, and demonstrates significant biological activity. This study assessed the therapeutic effects of litchi pericarp extract (LPE) on type 2 diabetes mellitus in db/db mice. The results showed that LPE ameliorated symptoms of glucose metabolism disorder, oxidative stress, inflammatory response, and insulin resistance in db/db mice. The mechanistic studies indicated that LPE activates adenosine 5‘-monophosphate (AMP)-activated protein kinase (AMPK) and suppresses the protein expression of phosphoenolpyruvate carboxykinase (PEPCK), thereby reducing hepatic gluconeogenesis. Additionally, LPE facilitates the translocation of nuclear factor erythroid2-related factor 2 (Nrf2) into the cell nucleus, initiating the transcription of antioxidant factors superoxide dismutase (SOD) and NAD(P)H: quinone oxidoreductase 1 (NQO1), which alleviate oxidative stress and reduce oxidative damage. Furthermore, LPE blocks nuclear factor kappa-B (NF-κB) nuclear translocation and subsequent inflammatory response initiation, thereby reducing inflammation. These findings indicate that LPE addresses type 2 diabetes mellitus by activating the AMPK energy metabolic pathway and regulating the Nrf2 oxidative stress and NF-κB inflammatory signaling pathways.
An AlphaFold Structure Analysis of COQ2 as Key a Component of the Coenzyme Q Synthesis Complex
Authors: Vargas-Pérez María de los Ángeles , Devos Damien Paul , López-Lluch Guillermo
Published: April 2024

Coenzyme Q (CoQ) is a lipidic compound that is widely distributed in nature, with crucial functions in metabolism, protection against oxidative damage and ferroptosis and other processes. CoQ biosynthesis is a conserved and complex pathway involving several proteins. COQ2 is a member of the UbiA family of transmembrane prenyltransferases that catalyzes the condensation of the head and tail precursors of CoQ, which is a key step in the process, because its product is the first intermediate that will be modified in the head by the next components of the synthesis process. Mutations in this protein have been linked to primary CoQ deficiency in humans, a rare disease predominantly affecting organs with a high energy demand. The reaction catalyzed by COQ2 and its mechanism are still unknown. Here, we aimed at clarifying the COQ2 reaction by exploring possible substrate binding sites using a strategy based on homology, comprising the identification of available ligand-bound homologs with solved structures in the Protein Data Bank (PDB) and their subsequent structural superposition in the AlphaFold predicted model for COQ2. The results highlight some residues located on the central cavity or the matrix loops that may be involved in substrate interaction, some of which are mutated in primary CoQ deficiency patients. Furthermore, we analyze the structural modifications introduced by the pathogenic mutations found in humans. These findings shed new light on the understanding of COQ2’s function and, thus, CoQ’s biosynthesis and the pathogenicity of primary CoQ deficiency.
Protective and Regenerative Effects of Reconstituted HDL on Human Rotator Cuff Fibroblasts under Hypoxia: An In Vitro Study
Authors: Kim Ra Jeong , Park Hyung Bin
Published: April 2024

Hypoxia and hypo-high-density lipoproteinemia (hypo-HDLemia) are proposed risk factors for rotator cuff tear. HDL is recognized for its potential benefits in ischemia-driven angiogenesis and wound healing. Nevertheless, research on the potential benefits of reconstituted HDL (rHDL) on human rotator cuff fibroblasts (RCFs) under hypoxia is limited. This study investigates the cytoprotective and regenerative effects of rHDL, as well as N-acetylcysteine (NAC), vitamin C (Vit C), and HDL on human RCFs under hypoxic conditions. Sixth-passage human RCFs were divided into normoxia, hypoxia, and hypoxia groups pretreated with antioxidants (NAC, Vit C, rHDL, HDL). Hypoxia was induced by 1000 µM CoCl2. In the hypoxia group compared to the normoxia group, there were significant increases in hypoxia-inducible factor-1α (HIF-1α), heme oxygenase-1 (HO-1), and Bcl-2/E1B-19kDa interacting protein 3 (BNIP3) expressions, along with reduced cell viability, elevated reactive oxygen species (ROS) production, apoptosis rate, expressions of cleaved caspase-3, cleaved poly ADP-ribose polymerase-1 (PARP-1), vascular endothelial growth factors (VEGF), and matrix metalloproteinase-2 (MMP-2), as well as decreased collagen I and III production, and markedly lower cell proliferative activity (p ≤ 0.039). These responses were significantly mitigated by pretreatment with rHDL (p ≤ 0.046). This study suggests that rHDL can enhance cell proliferation and collagen I and III production while reducing apoptosis in human RCFs under hypoxic conditions.
Redox Regulation of LAT Enhances T Cell-Mediated Inflammation
Authors: James Jaime , Coelho Ana , Lahore Gonzalo Fernandez , Hernandez Clara M. , Forster Florian , Malissen Bernard , Holmdahl Rikard
Published: April 2024

The positional cloning of single nucleotide polymorphisms (SNPs) of the neutrophil cytosolic factor 1 (Ncf1) gene, advocating that a low oxidative burst drives autoimmune disease, demands an understanding of the underlying molecular causes. A cellular target could be T cells, which have been shown to be regulated by reactive oxygen species (ROS). However, the pathways by which ROS mediate T cell signaling remain unclear. The adaptor molecule linker for activation of T cells (LAT) is essential for coupling T cell receptor-mediated antigen recognition to downstream responses, and it contains several cysteine residues that have previously been suggested to be involved in redox regulation. To address the possibility that ROS regulate T cell-dependent inflammation through LAT, we established a mouse strain with cysteine-to-serine mutations at positions 120 and 172 (LATSS). We found that redox regulation of LAT through C120 and C172 mediate its localization and phosphorylation. LATSS mice had reduced numbers of double-positive thymocytes and naïve peripheral T cells. Importantly, redox insensitivity of LAT enhanced T cell-dependent autoimmune inflammation in collagen-induced arthritis (CIA), a mouse model of rheumatoid arthritis (RA). This effect was reversed on an NCF1-mutated (NCF1m1j), ROS-deficient, background. Overall, our data show that LAT is redox-regulated, acts to repress T cell activation, and is targeted by ROS induced by NCF1 in antigen-presenting cells (APCs).
The Role of NOX2-Derived Reactive Oxygen Species in the Induction of Endothelin-Converting Enzyme-1 by Angiotensin II
Authors: Adu-Gyamfi Michael , Goettsch Claudia , Kamhieh-Milz Julian , Chen Lei , Pfefferkorn Anna Maria , Hofmann Anja , Brunssen Coy , Müller Gregor , Walther Thomas , Ashraf Muhammad Imtiaz , Morawietz Henning , Witowski Janusz , Catar Rusan
Published: April 2024

Endothelin-1 is a key regulator of vascular tone and blood pressure in health and disease. We have recently found that ET-1 production in human microvascular endothelial cells (HMECs) can be promoted by angiotensin II (Ang II) through a novel mechanism involving octamer-binding transcription factor-1 (Oct-1), NADPH oxidase-2 (NOX2), and superoxide anions. As the formation of bioactive ET-1 also depends on endothelin-converting enzyme-1 (ECE-1), we investigated the transcriptional regulation of the ECE1 gene. We found that exposure of HMECs to Ang II resulted in a concentration- and time-dependent increase in ECE1 mRNA expression. Pharmacological inhibition of ECE-1 reduced Ang II-stimulated ET-1 release to baseline values. The effect of Ang II on ECE1 mRNA expression was associated with Oct-1 binding to the ECE1 promoter, resulting in its increased activity. Consequently, the Ang II-stimulated increase in ECE1 mRNA expression could be prevented by siRNA-mediated Oct-1 inhibition. It could also be abolished by silencing the NOX2 gene and neutralizing superoxide anions with superoxide dismutase. In mice fed a high-fat diet, cardiac expression of Ece1 mRNA increased in wild-type mice but not in Nox2-deficient animals. It can be concluded that Ang II engages Oct-1, NOX2, and superoxide anions to stimulate ECE1 expression in the endothelium.