bluuebunny/crossref_metadata_2025 huggingface dataset:
Autoimmune hepatitis and myositis as presenting manifestation of mixed connective tissue disease – A case report
Authors: Yadav Praveen Kumar , Maurya Surya Kant
Published: October 2023

In the course of mixed connective tissue disease (MCTD), hepatitis is very rare. Most commonly liver steatosis or elevated liver enzymes are reported and only a few cases of MCTD associated with autoimmune hepatitis were described previously. We report a case of 30-year-old female, who initially presented with autoimmune hepatitis and was treated with oral Prednisolone. Few weeks later, she presented with lower motor neuron weakness of all four limbs with normal reflexes and sensory findings. Clinically, the pattern of weakness was suggestive of a myopathic process which was confirmed with a high creatine phosphokinase levels and electromyography. She had history of Raynaud’s phenomenon with minimal skin thickening involving the extremities and face. Thus, a diagnosis of overlap syndrome/MCTD was kept. MCTD has been confirmed on high titers positive anti U-1 ribonucleoprotein antibodies. Autoimmune hepatitis and myositis as the presenting manifestation are very rare. Here, we highlight a patient of MCTD with initial symptoms of autoimmune hepatitis and myositis.
Soil assessment for heavy metal contamination and potential ecological risks
Authors: Garg Vijay Kumar , Srivastav Arun Lal , Tiwari M.K. , Sharma Ajay , Kanwar Varinder Singh
Published: October 2023

Pollution in soil (particularly heavy metal contamination) is a result of industrialization, urbanization, intensified use of fertilisers, insecticides and irrigation water. The quality of agricultural soil in the vicinity of the industry is of wide concern due to associated health risks caused by presence of heavy metals in it. Heavy metals pollution in the soil leads to its poor health, groundwater and food contamination which is hazardous to human health. In the present study, wavelength dispersive X-ray fluorescence (WDXRF) technique has been used for soil assessment in one of the industrial areas of Himachal Pradesh, India. The XRF results show a larger concentration of some of the heavy metals in different soil samples as compared to the permissible values. The high values of contamination factor especially for titanium (Ti), vanadium (V), chromium (Cr), cobalt (Co) and barium (Ba) show that the contamination in the agricultural soils in the vicinity of the industries is due to their shear negligence. The investigated contamination factor lies in the range; 3.79Cfi<14, 3.29Cfi<17.86, 1.64Cfi<18.96, 2.78Cfi<6.22 and 2.38Cfi<6.30 for Ti, V, Cr, Co and Ba respectively. Moreover, the results indicate that the risk index ranges from moderate potential risk to considerable potential risk.
An in-depth and in vitro evaluation of the antioxidant and neuroprotective activity of aqueous and ethanolic extract of Asparagus racemosus Linn seed
Authors: Dubey Anubhav , Ghosh Niladry , Singh Ranjit
Published: October 2023

The study aimed to explore the phytochemicals present in the aqueous and ethanolextracts of Asparagus racemosus Linn seeds by GC-MS and LC-MS analysis and to determine the neuro protective and antioxidant potential of both the extracts. The GC-MS and LC-MS analysis of the aqueous and ethanol seed extract indicated the presence of various chemical constituents such as ascorbic acid, hydroxycinnamic acid, fatty acids, pyroglutamic acid, pentanoic acid, decanoic acid, hexadecanoic acid, naphthalene, hydroxycinnamic acid, thiamine hydrochloride, coumaroyl putrescin, agrimol, epanorin, brevicarine, rubiadin, pyroglutamic acid, tyrosine derivative, linoleyl acetate etc. Neuroprotective effect of aqueous and ethanol extracts of Asparagus racemosus Linn seeds on SHSY5Y cell line was evaluated by MTT assay and the results showedthat ethanol extract of the plant got more neuroprotective activity on cell lines. The antioxidant results showed that both of the extracts had almost similar DPPH and ABTS radical scavenging. However, the ethanol extract had more potent antioxidant power than the aqueous extract. Study data suggest that whole plant of Asparagus racemosus Linn seeds possessed neuroprotective and anti-oxidative activities and the best neuroprotective and anti-oxidant activity being exhibited by the ethanolic extract of the plant.
Allelopathic effects of stems extracts of Mimosa Hamata Willd.
Authors: Singh Alok Kumar , Yadava R.N. , Yadav Ritu , Shrivastava S.P.
Published: October 2023

The allelopathic effect of aqueous stems extracts of Mimosa hamata Willd. Seed germination was studied using Vigna radiate L., Zea mays L. and Cicer arietinum L. seeds. The results of the investigation suggested that aqueous stems extracts of Mimosa hamata Willd. increased the percentage of germination and seedling vigor index increased. The aqueous stems extracts show allelopathic activity because there are a large number of bioactive compounds such as saponin which is also known as allelochemical present in the aqueous stems extracts of Mimosa hamata Willd. They have shown a positive allelopathic effect on stems from Mimosa hamata Willd.
In silico docking studies of alpha-amylase inhibitors from the methanol extract of Aloe vera
Authors: Karingula Naveen , Poreddy Guru Lakshmi , Dubey Akanksha , Sivaraman Jayanthi
Published: October 2023

In the current study, potent inhibitors of a-amylase, one of the significant regulating enzymes in diabetes, were identified from the methanolic extract of Aloe barbadensis miller (Aloe vera), an indigenous herbal plant used to treat carbohydrate absorption problems such as diabetes and obesity. The Aloe vera gel extract was dried, ground, macerated with methanol and subjected to phytochemical screening, which was characterized by gas chromatography. Since there is a rapid increase in computational methods to predict the association between two molecules, molecular docking studies assessed the effect of AVM (Aloe vera methanol) extract in inhibiting the enzyme alpha-amylase. The molecules derived from GC-MS analysis were employed as ligands for the receptor α-amylase in molecular docking experiments. The protein target used in this study was the human pancreatic α-amylase with PDB-ID -2QV4. The protein target and ligand structures were obtained from PDB and Pubchem databases respectively and docking studies were performed using PyRx. In this study, we intended to find distinct phytochemical substances from the Aloe vera plant as α-amylase inhibitors with an objective that the molecular docking studies provided in this study might result in the discovery of effective α-amylase inhibitors for diabetic management.
Features of situational teaching methods in china
Authors: Nikolaeva A. D.
Published: October 2023

Abstract. In modern conditions, the issue of adapting best educational practices from friendly countries, including China, as one of the countries with positive dynamics in improving the quality of education, becomes relevant. For quite a long time, in the pedagogical community and not only in China, the essence of learning was considered a process in which teachers, using their knowledge, skills and techniques, teach students with the active and conscious participation of the latter. Even then, learning was considered as a process of cognition, a special process that differs from other similar processes in people’s lives in terms of the object of cognition, speed, and methods. In the late 1950s, the idea arose in the pedagogical community that in the educational process it is necessary to follow the equation «practice – theory – practice». For this reason, many scholars in China began to conduct research on the essence of learning. The purpose and objectives of the study were to analyze the theory and practice of studying the learning process, including methods, in China. One of them is situational teaching methods. At the same time, methods of theoretical analysis, synthesis, interpretation, and generalization were used. Main results: in the course of the analysis, successful practices of using methods of teaching and educating students were identified, a characteristic was given and features of situational teaching methods based on the Concept of situational learning by Li Jilin were given. These methods are presented as methods of «live» learning, which contribute to the observance of the principle «teaching – educate».
The place of the digital «ecumene» in the system of teaching the Russian language to foreigners at neFU
Authors: Petrova S. M.
Published: October 2023

The article analyzes new trends in the development of modern Russian studies related to the search by methodologists for effective forms and means of teaching the Russian language to foreigners. The ways and techniques of developing modern scientific thinking for the practical study of the Russian language by foreigners are explored; attention is drawn to the creation of a new vector of methods for teaching the Russian language to foreigners, associated with the democratization of language norms; talks about the role of digital technologies in philological education and new directions of disciplinary science – digital humanities and its sections such as “corpus technologies in philology”, “big data in philology”, “corpus linguistics”, “national corpus of the Russian language”; the role of literature in the system of teaching the Russian language to foreigners using end-to-end digital technologies is pointed out, the ideas of creating a new teaching model related to the education of a citizen of the world, a bearer of ethnic consciousness and mentality are considered, the idea of the pedagogical value of cybertext as a new type of educational text is put forward. The article reveals the concept of “digital ecumene” in relation to the methodology of teaching RFL at NEFU, indicates its role and place in the system of teaching Russian to foreigners; the author’s method of teaching Russian to foreigners is shown using practice-oriented traditions of semiotics, mnemonics, ergonomics, in the system of graphic-semiotic analysis of a work of art. The use of this methodology will help make the educational process more intensive, will contribute to the activation of cognitive and mental activity, the formation of an understanding of professional competencies, and the expansion and deepening of students’ information culture.
RELATIVE CHRONOLOGY OF THE POLYANKA SETTLEMENT IN THE CRIMEAN PRIAZOVIA
Authors: Масленников А.А.
Published: October 2023

Практически на каждом античном поселении встречаются находки, как более ранние, так и более поздние, нежели основная их масса, связанная собственно с конкретными культурными напластованиями. Не исключение и городище Полянка в Крымском Приазовье (III–I вв. до н. э.). Здесь немногочисленные обломки амфор, а это самые «объективные» по своей характеристике и надежной хронологии артефакты, относятся к первой половине и концу V, второй или последней четвертям следующего века, II–III и, видимо, IV–VI вв. н. э. Появление их не только случайно, но и связано с разными причинами и обстоятельствами хозяйственного, географического или сакрального плана. Учет данного фактора важен для определения так называемой относительной хронологии того или иного памятника археологии при его постановке на соответствующий учет региональными органами их охраны.

Practically each Ancient Greek settlement yields a certain number of finds other than bulk finds both from the earlier and later periods directly related to specific occupation layers. The Polyanka fortified settlement in the Crimean Priazovia (3rd–1st centuries BC) is no exception. A small number of fragments of the amphorae retrieved from Polyanka serve to be the most ‘objective’ artifacts in terms of their characteristics and reliable chronology and date to the first half and the end of the 5th century, the second or the last quarter of the subsequent century and, apparently, to the 4th–6th centuries AD. Their appearance is not only incidental, but is also linked to various reasons and economical, geographical or sacral aspects. It is important to take this factor into account in determining the so called relative chronology of an archaeological site which is to be entered into the records of relevant regional agencies that work to protect archaeological heritage.
MITHRIDATIC HOARD OF BRONZE COINS FROM PHANAGORIA
Authors: Абрамзон М.Г. , Остапенко С.Н.
Published: October 2023

В 2022 г. во время археологических работ в Фанагории (раскоп «Нижний город») был найден небольшой клад бронзовых монет. Кошелек с десятью боспорскими и понтийскими монетами митридатовского времени был сокрыт в тайнике под небольшим камнем у фундамента разрушенного здания. За исключением двух халков, все монеты представляют собой оболы и тетрахалки, чеканенные в Пантикапее, Амисе, Хабакте, Синопе. Кошелек скорее всего принадлежал понтийско му наемнику и был спрятан во время антимитридатовского восстания в Фанагории в 63 г. до н. э.

During the archaeological excavations at Phanagoria (site “Lower City”), a small hoard of bronze coins was discovered. A purse containing ten Bosporan and Pon tic coins of the Mithradatic time was hidden in a cache under a small stone near the base of wall of the destroyed building. Apart from two chalkoy, all resting coins are the obols and tetrachalkoy minted at Panticapaeum, Amisus, Chabacta, and Sinope. The purse most likely belonged to a Pontic mercenary and was concealed during the anti-Mithradatic uprising in Phanagoria in 63 BC.
THE CHEMICAL-ANALYTICAL STUDY OF ANCIENT METAL: ISSUES OF CORRELATION AND DATA USE
Authors: Гак Е.И. , Луньков В.Ю. , Кузьминых С.В.
Published: October 2023

В статье дана оценка совместимости и потенциала разных аналитических методик по установлению химического (элементного) состава древней меди и ее сплавов. На примере инвентаря Потаповского могильника эпохи бронзы, в разное время проанализированного методами оптико-эмиссионной спектрометрии и спектрометрии с дисперсией по длине волны, показано отсутствие принципиальных расхождений в определении материала и явных системных сбоев работы того или другого оборудования. Близкий уровень корреляции парных данных зафиксирован и другими исследованиями, что делает эти данные одинаково пригодными для решения архео-металлургических задач.

The paper assesses compatibility and potential of various analytical methodologies employed to identify the chemical (elemental) composition of ancient copper and its alloys. As illustrated by the funerary offerings from the Potapovsky cemetery of the Bronze Age which were analyzed by various methods such as optical emission spectrometry and wavelength-dispersive spectrometry at different times, the analysis 279 Е. И. Г ак и др. found absence of fundamental differences in the material identified and obvious systemic operational failures of a specific equipment. Similar correlation of the paired data has been documented under other studies as well, which means that these data are suitable for ad dressing archaeometallurgical tasks.
CONTACTS OF THE RESIDENTS OF PRIVOLNOYE-1 SETTLEMENT ON THE KALININGRAD PENINSULA AT THE END OF THE 11th–13th CENTURIES
Authors: Прасолов Я.В. , Скворцов К.Н.
Published: October 2023

В статье представлены первые результаты исследования объекта археологического наследия «Селище Привольное-1» в 2016 и 2021 гг. Памятник расположен на севере Калининградского полуострова в границах древнего ареала прусского племени сембов и датируется по известному сегодня археологическому материалу концом ХI – началом XIII в. – т. н. поздне-языческим временем локальной хронологии. Селище выделяется на общем фоне средневековых поселений пруссов как необычно высокой насыщенностью культурного слоя индивидуальными и массовыми находками, так и самим характером последних. Наряду с артефактами, характерными для материальной культуры как средневековых пруссов, так и их соседей куршей, скальвов и ливов, а также свидетельствами торговли со средневековыми западноевропейскими государствами, впервые за всю историю исследования полуострова были обнаружены в контексте поселения многочисленные предметы круга древнерусских древностей. Данные находки свидетельствуют о наличии культурных, торговых и, очевидно, юридических взаимодействий между жителями поселения и Древней Руси в период между эпохой викингов и началом крестоносной экспансии Тевтонского ордена на земли пруссов.

The paper describes the first results of the investigation carried out at the Privolnoye-1 unfortified settlement, which is an archaeological heritage site, in 2016 and 2021. The site is located in the north of the Kaliningrad peninsula within the ancient area inhabited by the Prussian population known as the Sembians and, based on the currently available material, is dated to the end of the 11th – early 13th centuries, to the so called ‘Late Pagan Period’ of the local chronology. This unfortified settlement stands out among other Prussian medieval settlements because of the occupation layer with an exceptional abundance of individual and frequent finds as well as the nature of these finds. Along with the artifacts typical for the material culture of both medieval Prussians and their neighbors such as the Kurshi, the Skalvians, and the Livs and well as evidence of trade with medieval western European states, for the first time in the history of the excavations on the peninsula numerous items ascribed to Medieval Rus antiquities were discovered in the context of the settlement. These finds provide evidence of cultural, trading and, apparently, legal relationship between the settlement residents and the population of Medieval Rus in the period between the Viking Age and the startup of the crusader expansion of the Teutonic Order to the Prussian lands.
PROTECTION AND STUDIES OF THE MEDIEVAL ISKER HILLFORT DURING THE SOVIET PERIOD (EARLY 1920s – EARLY 1990s)
Authors: Балюнов И.В.
Published: October 2023

В существующих публикациях история исследований городища Искер в советское время представлена достаточно скупо. В них не были описаны процессы учета и охраны памятника, практически отсутствует информация о деятельности московских археологов и сотрудников Тобольского музея. В статье сделана попытка ликвидировать эти пробелы и ввести в научный оборот данные из неопубликованных документов. Сопоставление различных источников показывает, что научное изучение городища Искер продолжалось на протяжении всего времени, однако результаты такого изучения часто становились малодоступными. Благодаря проводимой работе памятник был поставлен на централизованный учет и государственную охрану, что, однако, не смогло предотвратить его дальнейшего разрушения.

The available publications contain rather scarce information on the history relating to investigations of the Isker hillfort during the Soviet period. These publications do not describe processes of documenting and protecting the site, there is practically no information on the activities undertaken by Moscow archaeologists and staff members of the Tobol museum. The paper attempts to eliminate these gaps and introduce data from unpublished documents into scientific discourse. The comparison of various sources demonstrates that the Isker hillfort was investigated using scientific methods during the entire Soviet period; however, the results of such investigations were not easily accessible. The work performed ensured that the site was recorded into the central register and placed under state protection, which, nevertheless, failed to prevent its subsequent decay and damage.
Target-Specific Novel Molecules with their Recipe: Incorporating Synthesizability in the Design Process
Authors: Ramaswamy Krishnan Sowmya , Bung Navneet , Srinivasan Rajgopal , Roy Arijit
Published: October 2023

Application of Artificial intelligence (AI) in drug discovery has led to several success stories in recent times. While traditional methods mostly relied upon screening large chemical libraries for early-stage drug-design, the AI-based approaches can help identify novel target-specific molecules by sampling from a much larger chemical space. Although this has increased the possibility of finding diverse and novel molecules from previously unexplored chemical space, this has also posed a great challenge for medicinal chemists to synthesize at least some of the AI-designed novel molecules for experimental validation. To address this challenge, in this work, we propose a novel forward synthesis-based generative AI method, which is used to explore the synthesizable chemical space. The method uses a structure-based drug design framework, where the target protein structure and a target-specific seed fragment from co-crystal structures can be the initial inputs. A random fragment from a purchasable fragment library can also be the input if a target-specific fragment is unavailable. Then a template-based forward synthesis route prediction and molecule generation is performed in parallel using the Monte Carlo Tree Search (MCTS) method where, the subsequent fragments for molecule growth can again be obtained from a purchasable fragment library. To the best of our knowledge, this is the first model to utilize MCTS for forward synthesis route prediction. The rewards for each iteration of MCTS are computed using a drug-target affinity (DTA) model based on the docking pose of the generated reaction intermediates at the binding site of the target protein of interest. With the help of the proposed method, it is now possible to overcome one of the major obstacles posed to the AI-based drug design approaches through the ability of the method to design novel target-specific synthesizable molecules.
The Missing Link for Electrochemical CO2 Reduction: Classification of CO vs. HCOOH Selectivity via PCA, Reaction Pathways and Coverage Analysis
Authors: Christensen Oliver , Bagger Alexander , Rossmeisl Jan
Published: October 2023

For the electrochemical CO2 reduction reaction, different metal catalysts produce different products preferentially. However, the differences between the metals' reaction pathways that lead to these different products is still not fully understood. In this work, we analyze CO vs. HCOOH formation from CO2 using statistical analysis and DFT calculations. This is carried out by considering multiple descriptors, along with the competing reaction pathways, reaction barriers, and high coverage of mixed adsorbates on the surface. This method is capable of explaining the discrepancy between simulations and experiments regarding Ag and Au selectivity, and of properly classifying elements according to their product distribution. We find that, when considering water-assisted protonation for the disproportionation to CO, Ag and Au have a lower barrier for CO production in agreement with experimental results. We also find that, when considering high coverage of mixed adsorbates on the Ag/Au surface, the most stable adsorbate configuration contains adsorbates capable of forming CO preferentially. These findings help to bridge the gap between simulations and experiments and provide a missing link for our understanding of the CO2 reduction reaction.
پێکهاتەی ڕستەی بکەرنادیار لە شێوەزاری کەلەکچیدا
Authors: حجی ماهر , عەبوزەید سانیا
Published: October 2023

ئەم لێکۆلینەوەیە ژێر ناونیشانی (پێکهاتەی ڕستەی بکەرنادیار لە شێوەزاری کەلەکچیدا) یە، لەم لێکۆلینەوەدا هەولدراوە بە شێوەیەکی گشتی پێکهاتەی ڕستەی بکەرنادیاری شێوەزاری کەلەکچی دیاربکرێت، هەروەها ئاماژە بە تایبەتمەندییە سینتاکسییەکانیانی ئەم پێکهاتەیە دەکرێت و شرۆڤە دەکرێت. لە هەموو زمانێکدا بکەر پێکهێنەکی سەرەکییە لە ڕستەدا، پێویستە بکەر بۆ ئەنجامدان یان ڕوودانی کار لە ڕستەدا هەبێت، جا بکەر کەسێک بێت یان شتێک بێت، کەواتە بکەر لە پێناو تەواوکردنی ڕستەیە لە ڕووی سینتاکسیی و واتاییەوە، بەلام هەندێک جار بکەر لە ڕستەدا وون دەکرێت، بەم حالەتە دەوترێت (بکەرنادیار). گرنگی ئەم لێکۆلینەوەیە لەوەدایە؛ بۆ یەکەمجارە ئاماژە بە پێکهاتەی ڕستەی بکەرنادیاری شێوەزاری کەلەکچی دەکرێت و شێوە و جۆر و یاسا و تایبەتمەندییەکانی پێکهاتەی ڕستەی بکەرنادیار لەم شێوازەدا دیارکراوە و لە یەکجیاکراونەتەوە. ئەم لێکۆلینەوەیە بە پێی ڕێبازی وەسفی شیکاری ئەنجامدراوە، بابەتەکە وەسفکراوە و شێوە و جۆرەکانی پیکهاتەی بکەرنادیاری بەپێی ڕێژە و تاف و رووکارەکانی کاری ڕستە  شیکراوەتەوە. گرنگترین پرسیارەکانی ئەم لێکۆلینە ئەوەیە، تایبەتمەندییە سینتاکسییەکانی پێکهاتەی ڕستەی بکەرنادیاری شێوەزاری کەلەکچی چییە؟ ئایا پێکهاتەی بکەرنادیار بە ڕێگای سینتاکسی یان مۆرفۆلۆجی دروستدەکرێت؟ یان بە هەر دوو ڕێگا ؟ لێکۆلینەوە لە سەر زمانی کوردی، زاری گۆران، شێوەزاری کەلەکچی کراوە، داتاکان لە زمانی ئاخاوتنی ڕۆژانەی خەلکی ئەم شێوەزارە وەرگیراوە. لە کۆتاییدا  گرنگترین ئەو ئەنجامەی پێیگەیشتووینە، کە لەم شێوەزارەدا پێکهاتەی ڕستەی بکەرنادیار بە هەر دوو ڕێگا بەرهەمدەهێن.
Menstrual function and mental health of medical students during the COVID-19 pandemic: a continuous cross-sectional study
Authors: Gorbacheva Tatiana I. , Nikolaeva Maria G. , Stroganov Alexander E. , Orlova Olga V. , Khoreva Larisa A. , Uvarova Yulia M. , Denisyuk Ekaterina A. , Оловянников Yury S.
Published: October 2023

Aim. To assess the impact of new coronavirus infection (NCI) and COVID-19 vaccination on menstrual function in comparison with the frequency of depressive disorders during the COVID-19 pandemic among female students of a medical university.
Materials and methods. Data for a continuous transverse (cross-sectional) study were obtained using an online survey. The questions included demographics, characteristics of menstrual function, the history of COVID-19 and vaccinations against it, and the standard CES-D (Center for Epidemiologic Studies Depression Scale) questionnaire. The survey included 1.879 female medical students. The exclusion criteria were age under 18 and over 25 years, hormonal contraceptives, pregnancy, childbirth during the last year, and vaccination after COVID-19. After applying the exclusion criteria, three study groups were formed. Group 1 included female students with a history of NCI (n=140), group 2 included students with no history of NCI, who were initially vaccinated against COVID-19 (n=647), group 3 (control group) included unvaccinated students with no history of NCI (n=55).
Results. There were no differences in the prevalence of changes in menstrual function in female students after the NCI (49.3%) and after COVID-19 vaccination (39.6%) compared to the control group (43.6%); p=0.477. The rate of depression in the overall study cohort was 43.3%, without any significant differences between the study groups. Significant predictors of changes in menstrual function during the pandemic were marriage (odds ratio OR 2.33 [1.513.61]), depression (OR 1.72 [1.282.3]), a history of menstrual dysfunction (OR 1.5 [0.121.99]), and later menarche (OR 1.76 [1.023.04]). Multivariate analysis did not show the significance of the history of NCI and vaccination as factors of menstrual dysfunction (OR 1.61 [0.892.90] and OR 0.91 [0.591.41], respectively).
Conclusion. During the COVID-19 pandemic, female medical students reported frequent changes in menstrual function and depressive disorders. During the pandemic, the most significant predictors of menstrual disorders in female students were depression, a history of menstrual dysfunction, and marriage. A multicenter prospective study is necessary to clarify the mechanisms of the pandemic's impact on menstrual function.
The birth of a healthy child in a married couple with a history of multiple unsuccessful attempts at ART by selecting sperm on oocyte-cumulus complexes. Case report
Authors: Chistyakova Alina V. , Babayan Alina A. , Dovgan Alina А. , Makarova Natalya P. , Lobanova Natalya N. , Smolnikova Veronika Yu.
Published: October 2023

In male infertility, couples must use assisted reproductive technologies with the intracytoplasmic sperm injection (ICSI) technique. A significant disadvantage of ICSI is bypassing natural selection barriers since selective processes in the female reproductive tract are replaced by the choice of an embryologist based on sperm motility and morphological criteria. Various selection methods are being actively studied, bringing fertilization by ICSI closer to physiological, ensuring the selection of sperm with high DNA quality and good potential for fertilization. The article presents a clinical case of the birth of a healthy child in a married couple with severe male infertility and many ineffective attempts at assisted reproductive technologies using the method of sperm selection on oocyte-cumulus complexes. The child is physically healthy and develops according to age.
Uterine microbiome in women with uterine scar defect after cesarean section: Prospective cohort study
Authors: Michelson Anna A. , Telyakova Margarita I. , Lazukina Maria V. , Chistyakova Guzel N. , Ustyuzhanin Aleksandr V. , Varaksin Anatoly N. , Maslakova Tatyana A. , Konstantinova Ekaterina D.
Published: October 2023

Aim. To evaluate the endometrial microbiota in patients with uterine scar defect after cesarean section.
Materials and methods. The study included 80 women of reproductive age. The main group included 50 patients with a uterus scar defect due to cesarean section; the comparison group included 30 women with a competent uterus scar due to cesarean section. Patients underwent a Pipelle endometrial biopsy on days 2022 of the menstrual cycle using a double-lumen catheter that excludes sample contamination with vaginal and cervical microflora. A molecular genetic study of the endometrium was performed by real-time polymerase chain reaction using the Femoflor 16 reagent kit (DNA-Technology
Moscow). The DNA content in the specimen was measured using software and expressed in genome equivalent (GE) proportional to the number of microorganisms. The statistical data were processed using the Exсel software package and SPSS Statistics 22.0.
Results. The study showed the following statistically significant differences: lower count of Lactobacillus spp. 2.600 (1.4303.600) GE/mL vs 3.550 (2.8004.700) GE/mL in patients of the comparison group (p=0.02); higher count of Streptococcus and Staphylococcus spp. 3.270 (3.0003.700) GE/mL and 3.450 (3.2003.600) GE/mL vs 1.030 (0.7601.700) GE/mL and 0.560 (0.1201.200) GE/mL in the comparison group, respectively (p0.00001); higher count of Enterobacteriaceae 2.700 (1.7003.300) GE/mL vs 0.950 (0.6601.120) GE/mL in the comparison group (p0.00001); higher count of Gardnerella/Prevotellabivia/Porphyromonas spp. 2.310 (0.9303.480) GE/mL vs 1.000 (0.0001.860) GE/mL (p=0.003); higher count of Peptostreptococcus spp. 0.195 (0.0001.560) GE/mL vs 0.000 (0.000-0.000) GE/mL (p=0.032); Eubacterium spp. 1.355 (0.1002.460) GE/mL vs 0.000 (0.0001.560) GE/mL (p=0.040).
Conclusion. Endometrial dysbiosis in patients with a uterus scar defect after cesarean section due to the possible effects of microorganisms can be considered one of the leading causes of the formation of a uterus scar defect in the postoperative period.
Endometrial expression of FOX proteins (FOXA1 and FOXA2) in women of reproductive age with different endometrial thickness: prospective cohort comparative study
Authors: Aganezova Natalia V. , Aganezov Sergey S. , Gogichashvili Ksenia E. , Artemyeva Anna S. , Nyuganen Anna O.
Published: October 2023

Aim. To evaluate the expression of FOX proteins (FOXA1 and FOXA2) in the endometrium during the implantation window in women with a history of reproductive dysfunctions with different thickness of the endometrium.
Materials and methods. The prospective cohort comparative study was conducted. The main group included patients with "thin" endometrium (7 mm according to ultrasound on preovulatory days; n=52), the comparison group consisted of women with normal endometrial thickness (7 mm according to ultrasound; n=62; women of both groups with reproductive dysfunctions of unknown reason), the control group included 16 healthy fertile women. Aspiration biopsy of the endometrium was performed on the 68 days after ovulation, as well as venipuncture to obtain a sample of peripheral blood to determine the levels of sex steroids (estradiol E2 and progesterone P). A combined histological and immunohistochemical study of endometrial biopsies was performed.
Results. All women had ovulatory values of progesterone P16.1 nmol/l (68 days after ovulation) and normoestrogenemia (E2, pmol/l) in the blood. E2/P was similar in all groups (p0, 05). A pronounced expression of FOXA1 was noted in women with thin endometrium significantly more often (p0.05) with various hormone-receptor characteristics of the endometrium (42% n=22 out of 52) compared with healthy participants (0%; n=0). Reduced FOXA2 expression in the uterine mucosa was significantly more often detected on 68 days after ovulation in women with "thin" endometrium (56% n=29 of 52) than in women with normal endometrial thickness, both in women from the comparison group and in healthy women from the control group (p0.05). In a generalized analysis of the expression of FOX proteins in the endometrium on days 68 after ovulation, it was generally found that every second (50%; n=57 out of 114) women with a history of reproductive disorders (with a reduced and normal M-echo value) expression of proteomic markers differed from healthy women. In the case of "thin" endometrium, more than two thirds of patients (71%; n=37 out of 52) showed differences in endometrial expression of FOX proteins compared with women without a burdened reproductive history.
Conclusion. In the majority of women (71%) with a thin endometrium and a history of reproductive dysfunctions, the expression of FOX proteins in the endometrium differed from the control group. Overall, endometrial expression of FOX proteins, which is likely to be different from healthy women, in patients with reproductive dysfunctions of unknown origin is a significant predictor of reproductive failure. At the same time, such an isolated indicator as M-echo value 7 mm according to ultrasound data is not an absolute prognostic marker of endometrial receptivity disorders.
Asthenia in children: What's new for a pediatrician. A review
Authors: Lokshina Evelina E. , Savitskaya Natalia A. , Khmelkova Irina I.
Published: October 2023

Pediatricians incidentally treat patients with asthenia (asthenic syndrome AS, asthenic condition). The etiology of this disorder is highly diverse. Asthenia is a universal response of the body to various distresses, leading to energy deficiency. The most common AS symptoms are weakness, fatigue, sleep disturbances, increased irritability, muscle pain, headache, etc. AS, including post-infectious AS, significantly worsens the quality of life of the child and his family, so it is necessary to report the manifestations promptly to make a differential diagnosis with other diseases with similar presentation. There are many therapies to treat asthenia, including biologically active additives. Thus, recent clinical studies showed the effectiveness of Kidz syrup with choline, which improves the manifestations of asthenia, increases resistance to psycho-emotional stress, improves focus and memory, and helps to cope with increased mental stress.
Derangement of the infant microbiome development: what a pediatrician needs to know. A review
Authors: Zakharova Irina N. , Berezhnaya Irina V. , Dmitrieva Diana K.
Published: October 2023

The research on the microbiome and microbiota of different human ecological niches and their impact on human health showed a significant breakthrough in the last decade. It is known that minor microbial contamination is present even in the fetus in utero, inducing immunological resistance. Immediately after birth, the baby rapidly becomes colonized by maternal and environmental microorganisms. The mode of delivery impacts the number of microbes and species composition and subsequent changes in the infant's immune response. A high risk of early onset of atopic march has been reported in children born by cesarean section and treated with antibacterial drugs in early life. This paper reviews the results of recent studies of the effect of the probiotic based on the two most studied strains of Lactobacillus rhamnosus GG and Bifidobacterium lactis BS01 (Probielle Baby) on intestinal microbiota development and the prospects of its use for correcting disorders in children at high risk of atopy.
Phytotherapy of respiratory infections in children: Iceland moss syrup as a new treatment for dry cough. A review
Authors: Starostina Lada S. , Malakhov Alexander B.
Published: October 2023

Regardless of the season, cough is one of the most common reasons for visiting a doctor, especially during the period of a high incidence of acute respiratory infections and other diseases manifested by cough, including long-term cough (laryngitis, tracheitis, bronchitis, pneumonia, tuberculosis, asthma, and other diseases). Patients often seek medical advice at a pharmacy without first seeing a doctor. In most cases, the cough is a protective reflex that helps to clear the accumulated bronchial secretion; however, it can significantly worsen the patient's condition and quality of life in adults and children. It negatively impacts the quality of life when the cough is still dry and unproductive, or the sputum is very thick. The article addresses various aspects of using herbal medicinal products of the Herbion series for various cough types, including post-infectious cough. Evidence supports using herbal medicinal products for dry and productive cough and a novel product from the Herbion series, Iceland moss syrup, for treating dry cough, including in children from 1 year.
Editorial workflow of a community-led, all-volunteer scientific journal: lessons from the launch of Seismica
Authors: Mark Hannah , Ragon Théa , Funning Gareth , Hicks Stephen P. , Rowe Christie , Teplitzky Samantha , Convers Jaime , Karasözen Ezgi , Corona-Fernandez R. Daniel , Fagereng Åke
Published: October 2023

Seismica is a community-led, volunteer-run, diamond open-access journal for seismology and earthquake science, and Seismica's mission and core values align with the principles of Open Science. This article describes the editorial workflow that Seismica uses to go from a submitted manuscript to a published article. In keeping with Open Science principles, the main goals of sharing this workflow description are to increase transparency around academic publishing, and to enable others to use elements of Seismica's workflow for journals of a similar size and ethos. We highlight aspects of Seismica's workflow that differ from practices at journals with paid staff members, and also discuss some of the challenges encountered, solutions developed, and lessons learned while this workflow was developed and deployed over Seismica's first year of operations.
La tecnología serverless, una oportunidad para los ecosistemas tecnológicos de apoyo a la investigación
Authors: Valencia Rivera Andrés Felipe , Sepúlveda Rodríguez Luis Eduardo , Candela Uribe Christian Andrés
Published: October 2023

Los ecosistemas tecnológicos de apoyo a la investigación (ETAI), son ambientes integrados por diversos actores dedicados al desarrollo de ciencia y tecnología, entre ellos, universidades, laboratorios, centros de investigación, industrias e institutos privados, quienes emplean un conjunto heterogéneo de recursos y servicios informáticos (RSI). Esta investigación centra su atención en los ETAI de países en vía de desarrollo, especialmente aquellos que presentan dificultades en la visibilidad y acceso de los RSI limitando así su uso compartido, lo que puede manifestarse en la duplicación de esfuerzos para la puesta a punto de los ambientes especializados de computación, y la adquisición redundante de RSI, en lugar de usar los existentes de forma compartida. Por su parte, serverless es una tecnología donde los desarrolladores pueden crear servicios sin preocuparse por los servidores (administración, configuración y mantenimiento), es decir, la infraestructura subyacente es controlada por el proveedor del servicio, quien se encarga de habilitar y escalar la infraestructura automáticamente de acuerdo con la demanda. En este contexto, esta investigación busca identificar oportunidades para la visibilidad, acceso y uso compartido de los RSI, a partir del estudio de la tecnología serverless y su beneficio potencial en los flujos de trabajo científico de los ETAI. Metodológicamente, se elaboró una revisión de la literatura a través de un estudio de mapeo sistemático (SMS por sus siglas en inglés – Systematic Mapping Study) con la finalidad de identificar investigaciones relacionadas con la ejecución de flujos de trabajo usando tecnología serverless en los ETAI, cuyo resultado permitió conformar en gran medida el estado del arte de la investigación. A partir de este estado del arte, se realizó un proceso formal de toma de decisiones para seleccionar una tecnología serverless, en la cual se desarrolló un prototipo funcional que incluyó un clúster de kubernetes, empleando infraestructura de la Universidad Tecnológica de Pereira (UTP). Finalmente, en esta investigación se concluye que la tecnología serverless es una oportunidad para la comunidad de los ETAI, debido a que facilita el trabajo colaborativo entre diferentes grupos de investigación, ofreciendo la posibilidad de desarrollar funciones para la creación de flujos de trabajo científico. Estas funciones pueden ser producto de los descubrimientos y hallazgos de cada grupo de investigación, las cuales estarían disponibles para ser usadas a través del ETAI. Asimismo, es viable que los RSI de los ETAI se configuren con alguna tecnología serverless, habilitando su visibilidad y acceso compartido, para ser empleados como ambiente de computación a disposición de otros ETAI.
Incorporación de las dimensiones internacionales e interculturales en el currículo del programa de ingeniería ambiental de la Unidad Central del Valle del Cauca como aporte a la transformación significativa del territorio
Authors: Rodríguez Correa Alejandra María , Buitrago González María Eugenia , Santacoloma Sandra Patricia , Cobo Mejía Germán
Published: October 2023

La Unidad Central del Valle del Cauca – UCEVA con su modelo pedagógico interestructurante con enfoque integrador, promueve procesos de enseñanza – aprendizaje con impacto significativo en el desarrollo regional y nacional, con perspectiva global. Parte del reconocimiento intercultural de las comunidades y busca la transformación de sus territorios en el marco de la justicia y el buen vivir; promoviendo la formación de profesionales con saberes y conocimientos evidenciados en los resultados de aprendizaje y competencias propias de la ingeniería ambiental, impactando así a la sociedad y aportando a la solución de problemáticas ambientales. Este trabajo tuvo como propósito articular la internacionalización solidaria y la dimensión intercultural en el currículo del programa de Ingeniería Ambiental de la UCEVA, mediante el desarrollo curricular que involucró la planeación, desarrollo y ejecución de procesos y proyectos de docencia, investigación y proyección a la comunidad. En la primera fase se realizó el reconocimiento de la diversidad social, ambiental, cultural y ancestral en la región, y la experiencia de los actores sociales, institucionales, gubernamentales y académicos. En la segunda fase, se orientaron procesos de enseñanza - aprendizaje hacia la generación de conocimientos, habilidades, valores y actitudes en los estudiantes y grupos de interés, y se formularon proyectos de investigación y proyección a la comunidad. En la tercera fase se ejecutaron procesos y proyectos establecidos en la segunda fase con la participación de estudiantes, instituciones, organizaciones y comunidades campesinas e indígenas. Entre los resultados del trabajo se encuentran la articulación y participación de las instituciones de educación superior partícipes en la Red de Conocimiento, Estudio e Investigación en Gestión Ambiental y Recursos Naturales “Red CEIGAR”, instituciones gubernamentales, autoridad ambiental y actores sociales. El diseño y planeación del Curso Internacional en Gestión Ambiental, Desarrollo Sustentable y el Buen Vivir. Encuentro e intercambio intercultural de actores. Reconocimiento de territorios ancestrales. Intercambio académico e investigativo entre IES. Movilidad entrante y saliente de docentes y estudiantes a la UCEVA. Participación en eventos de socialización del conocimiento.
Enhanced COVID-19 pneumonia diagnosis through optimized transformers and chest X-rays: post-pandemic insights
Authors: Escobar Ortiz Andrés
Published: October 2023

The COVID-19 pandemic led to an intense global health and economic impact, prompting researchers to seek rapid and effective diagnostic tools, such as Chest X-rays (CXR), especially in regions with limited resources. To alleviate the burden of manual CXR analysis, our study focuses on developing an optimized deep learning model to discern COVID-19 infections from other respiratory illnesses. We introduced an advanced screening tool comprising a Convolutional Neural Network (CNN) equipped with an optimized attention module. The training and validation of the model used a comprehensive private dataset of 5572 CXR images, illustrating various COVID-19 pneumonia severities, other pneumonia types, and healthy cases. Performance metrics showed training accuracy varying between 87% and 99.7%, with validation accuracy ranging from 87% to 93%. These results highlight the influence of dataset composition, especially the distribution of COVID-19 severity sub-classes, on the model's effectiveness. In the testing phase, the model performed optimally with a data set configuration rich in COVID-19 images, encompassing all sub-classes (sensitivity: 83%, false-negative rate: 17%). This study underscores the vital role of interdisciplinary cooperation in delivering expedient screening solutions during health crises. The proposed model achieved over 90% accuracy in identifying COVID-19 cases in CXRs, providing novel insights into the potential of imaging configurations for enhanced detection and differentiation of COVID-19 severities from other pneumonia instances.
La realidad aumentada en el arte: una apuesta para la reactivación de zonas de posconflicto
Authors: Ceballos Uribe Sebastián
Published: October 2023

La realidad aumentada ha permitido mejorar la forma en que las personas conocen e interactúan con el entorno. Del mismo modo, el arte a través del muralismo es una forma creativa para transmitir los sentimientos e historias de las comunidades. Este documento explica cómo se desarrolló una aplicación de realidad aumentada a partir de la sistematización de la historia y las experiencias plasmadas en los murales del Municipio de San Carlos en el departamento de Antioquia, y la cual sea una herramienta que permita la reactivación de los diferentes sectores económicos del municipio a través del turismo, dignificando a sus habitantes y lo cual genere una cultura de no repetición del conflicto.