bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Laccase and Transglutaminase on the Physicochemical and Functional Properties of Hybrid Lupin and Whey Protein Powder
Authors: Santoso Teguh , Ho Thao M. , Vinothsankar Geerththana , Jouppila Kirsi , Chen Tony , Owens Adrian , Lazarjani Masoumeh Pourseyed , Farouk Mustafa M. , Colgrave Michelle L. , Otter Don , Kam Rothman , Le Thao T.
Published: July 2024

Plant-based protein is considered a sustainable protein source and has increased in demand recently. However, products containing plant-based proteins require further modification to achieve the desired functionalities akin to those present in animal protein products. This study aimed to investigate the effects of enzymes as cross-linking reagents on the physicochemical and functional properties of hybrid plant- and animal-based proteins in which lupin and whey proteins were chosen as representatives, respectively. They were hybridised through enzymatic cross-linking using two laccases (laccase R, derived from Rhus vernicifera and laccase T, derived from Trametes versicolor) and transglutaminase (TG). The cross-linking experiments were conducted by mixing aqueous solutions of lupin flour and whey protein concentrate powder in a ratio of 1:1 of protein content under the conditions of pH 7, 40 °C for 20 h and in the presence of laccase T, laccase R, or TG. The cross-linked mixtures were freeze-dried, and the powders obtained were assessed for their cross-linking pattern, colour, charge distribution (ζ-potential), particle size, thermal stability, morphology, solubility, foaming and emulsifying properties, and total amino acid content. The findings showed that cross-linking with laccase R significantly improved the protein solubility, emulsion stability and foaming ability of the mixture, whereas these functionalities were lower in the TG-treated mixture due to extensive cross-linking. Furthermore, the mixture treated with laccase T turned brownish in colour and showed a decrease in total amino acid content which could be due to the enzyme’s oxidative cross-linking mechanism. Also, the occurrence of cross-linking in the lupin and whey mixture was indicated by changes in other investigated parameters such as particle size, ζ-potential, etc., as compared to the control samples. The obtained results suggested that enzymatic cross-linking, depending on the type of enzyme used, could impact the physicochemical and functional properties of hybrid plant- and animal-based proteins, potentially influencing their applications in food.
Development of a Freshness Indicator for Assessing the Quality of Packaged Pork Products during Refrigerated Storage
Authors: Lee Ga-Young , Lim Kyung-Jik , Lee Yoon-Hee , Shin Han-Seung
Published: July 2024

A pH-sensitive dye-based freshness indicator has been developed to monitor the quality status of pork neck through distinct color transitions, addressing a crucial need for improved food safety and real-time monitoring within the food industry. This system aims to boost consumer confidence and improve shelf-life estimates by offering transparent and immediate quality indicators. Aerobically packaged pork neck samples underwent accelerated testing at 25 °C for 36 h, followed by refrigeration experiments at typical distribution temperatures of 4 and 8 °C over 10 days. Measured pork neck quality parameters included total bacterial count (TBC), total volatile basic nitrogen (TVB-N), and pH levels. Visual observation and colorimetric analysis were used to assess the chromatic variations of the freshness indicator, which showed a significant shift from orange to green in response to the presence of TVB-N in the headspace of the pork packaging. The chromatic parameters of the freshness indicator exhibited a significant correlation with the pork quality values throughout the storage periods. The results highlight the ability of the freshness indicator to effectively convey quality information about pork through noticeable colorimetric changes.
Dynamical Behavior of the Fractional BBMB Equation on Unbounded Domain
Authors: Zhang Wei , Wang Haijing , Zhang Haolu , Li Zhiyuan , Li Xiaoyu
Published: July 2024

The fractional-order Benjamin-Bona-Mahony-Burgers (BBMB) equation is a generalization of the classical BBMB equation. It’s dynamic behaviors is much more complex than that of the corresponding integer-order BBMB equation. The main purpose of this paper is to explore the dynamic behaviors of the fractional-order BBMB equations by using the Fourier spectral method. Firstly, the numerical solution is compared with the exact solution. It is proved that the proposed method is effective and high precision for solving the spatial fractional order BBMB equation. Then, some dynamical behaviors of fractional order BBMB equations are obtained by using the present method, and some novel fractal waves of the the fractional-order BBMB equation on unbounded domain are shown.
Solving Fractional Random Differential Equations by Using Fixed Point Methodologies under Mild Boundary Conditions
Authors: Hammad Hasanen A. , Aljurbua Saleh Fahad
Published: July 2024

This manuscript aims to study the existence and uniqueness of solutions to a new system of differential equations. This system is a mixture of fractional operators and stochastic variables. The study has been completed under nonlocal functional boundary conditions. In the study, we used the fixed-point method to examine the existence of a solution to the proposed system, mainly focusing on the theorems of Leray, Schauder, and Perov in generalized metric spaces. Finally, an example has been provided to support and underscore our results.
The First VHE Activity of OJ 287 and the Extragalactic Background Light
Authors: Yadav Sameer , Kushwaha Pankaj
Published: July 2024

The BL Lacertae (BL Lac) object OJ 287 underwent an intense X-ray activity phase, exhibiting its brightest recorded X-ray flare in 2016–2017, characterized by much softer X-ray spectra and, concurrently, its first-ever recorded very-high-energy (VHE) emission (100–560 GeV), reported by the VERITAS observatory. Broadband spectral energy distribution reveals a new jet emission component similar to high-synchrotron-peaked BL Lac objects, thereby implying the soft X-ray spectrum for the synchrotron emission. Using the advantage of simultaneous X-ray and VHE spectral information, as well as the source being a low-synchrotron-peaked BL Lac object, we systematically explored the extragalactic background light (EBL) spectrum by demanding that the VHE spectrum cannot be harder than the X-ray spectrum. We used three different phenomenological forms of the EBL spectral shape (power-law, parabola, and polynomial) motivated by current constraints on the EBL with the Bayesian Monte Carlo approach to infer the credible EBL range. Our study favors an almost flat power-law spectral shape and is consistent with previous studies. The other spectral forms capable of capturing curvature though result in a better statistics value; the improvement is statistically insignificant given the additional parameters.
Hydrogel-Based Novel Biomaterials: Achievements and Prospects
Authors: Serro Ana Paula , Silva Diana Cristina , Fernandes Ana Isabel
Published: July 2024

In recent decades, hydrogels have garnered significant attention, thanks to their extensive biomedical and pharmaceutical applications [...]
Biomimetic Hydrogel Strategies for Cancer Therapy
Authors: Alshehri Awatef M. , Wilson Otto C.
Published: July 2024

Recent developments in biomimetic hydrogel research have expanded the scope of biomedical technologies that can be used to model, diagnose, and treat a wide range of medical conditions. Cancer presents one of the most intractable challenges in this arena due to the surreptitious mechanisms that it employs to evade detection and treatment. In order to address these challenges, biomimetic design principles can be adapted to beat cancer at its own game. Biomimetic design strategies are inspired by natural biological systems and offer promising opportunities for developing life-changing methods to model, detect, diagnose, treat, and cure various types of static and metastatic cancers. In particular, focusing on the cellular and subcellular phenomena that serve as fundamental drivers for the peculiar behavioral traits of cancer can provide rich insights into eradicating cancer in all of its manifestations. This review highlights promising developments in biomimetic nanocomposite hydrogels that contribute to cancer therapies via enhanced drug delivery strategies and modeling cancer mechanobiology phenomena in relation to metastasis and synergistic sensing systems. Creative efforts to amplify biomimetic design research to advance the development of more effective cancer therapies will be discussed in alignment with international collaborative goals to cure cancer.
Factors Associated with Mental Illness in a Primary Healthcare Setting in the Kingdom of Saudi Arabia: A Case–Control Study
Authors: Alangari Abdulaziz S. , Fatani Faris , Binhowaimel Nouf , Al Kadri Hanan M. , Alshahrani Awad , Al Khateeb Badr F. , Aldubikhi Aljohrah I. , Bin Amer Mona I. , Alsaif Afrah , El-Metwally Ashraf
Published: July 2024

Background/Objectives: Mental health conditions are a leading cause of morbidity and mortality worldwide, with a 13% rise within the last decade. This study aimed to investigate the factors associated with mental illness in patients presenting to a primary healthcare center in the Kingdom of Saudi Arabia (KSA). Methods: Data were extracted from the electronic health records of 46 primary care centers in Riyadh, Saudi Arabia from March 2022 to March 2023. A total of 2418 age-matched mental health cases and controls were evaluated. Descriptive and logistic regression analyses were performed to examine the distribution and association of relevant risk factors. A total of 1209 cases were age-matched to 1209 controls presenting to a primary clinic. Results: The odds of mental illness in females were 2.2 times that of males. Clinical conditions associated with mental illness were stroke, diabetes, and obesity. Neurodegenerative disorders were also associated with mental illness. Conclusions: Primary clinics in the KSA can serve as focal points in removing the barriers to mental healthcare. There is an urgent need to spread awareness, remove the stigma, and provide appropriate care and referrals for mental health conditions in the KSA.
A Culture of Early Mobilization in Adult Intensive Care Units: Perspective and Competency of Physicians
Authors: Albarrati Ali , Aldhahi Monira I. , Almuhaid Turki , Alnahdi Ali , Alanazi Ahmed S. , Alqahtani Abdulfattah S. , Nazer Rakan I.
Published: July 2024

Background: Early mobility (EM) is vital in the intensive care unit (ICU) to counteract immobility-related effects. A multidisciplinary approach is key, as it requires precise initiation knowledge. However, physicians’ understanding of EM in adult ICU settings remains unexplored. This study was conducted to investigate the knowledge and clinical competency of physicians working in adult ICUs toward EM. Methods: This cross-sectional study enrolled 236 physicians to assess their knowledge of EM. A rigorously designed survey comprising 30 questions across the demographic, theoretical, and clinical domains was employed. The criteria for knowledge and competency were aligned with the minimum passing score (70%) stipulated for physician licensure by the medical regulatory authority in Saudi Arabia. Results: Nearly 40% of the respondents had more than 5 years of experience. One-third of the respondents received theoretical knowledge about EM as part of their residency training, and only 4% of the respondents attended formal courses to enhance their knowledge. Almost all the respondents (95%) stated their awareness of EM benefits and its indications and contraindications and considered it safe to mobilize patients on mechanical ventilators. However, 62.3% of the respondents did not support EM for critically ill patients on mechanical ventilators until weaning. In contrast, 51.7% of respondents advised EM for agitated patients with RASS > 2. Only 113 (47.9%) physicians were competent in determining the suitability of ICU patients for EM. For critically ill patients who should be mobilized, nearly 60% of physicians refused to initiate EM. Conclusions: This study underscores insufficient practical knowledge of ICU physicians about EM criteria, which leads to suboptimal decisions, particularly in complex ICU cases. These findings emphasize the need for enhanced training and education of physicians working in adult ICU settings to optimize patient care and outcomes in critical care settings.
Acquisition and Retention Effects of Fundamental Movement Skills on Physical Activity and Health-Related Fitness of Children with Autism Spectrum Disorder
Authors: Xing Yu , Liu Haoyan , Wu Xueping
Published: July 2024

This study adopted a quasi-experimental design to explore the effects of fundamental movement skill intervention on the acquisition and retention of physical activity levels and health-related fitness in children with autism spectrum disorder (ASD). In the experiments, 11 children received fundamental motor skill training (12 weeks, 60 min/session, 4 times/week), and 10 children maintained traditional physical activity. Assessments were performed using an ActiGraph GT3X+ accelerometer, health-related fitness pre–post intervention, and 1-month follow-up tests. The sedentary time during physical activity was significantly decreased (p = 0.01), and there were large changes in health-related physical fitness indicators, including significantly improved body composition (body mass index, F(1,19) = 8.631, p = 0.03, partial η2 = 0.312), muscle strength and endurance (sit-ups, F(1,19) = 3.376, p = 0.02, partial η2 = 0.151 and vertical jumps, F(1,19) = 5.309, p = 0.04, partial η2 = 0.218), and flexibility (sit and reach, F(1,19) = 36.228, p = 0.02, partial η2 = 0.656). Moreover, the follow-up tests showed that the children’s sedentary time continued to reduce, and the muscle strength and endurance (sit-ups, F(1,19) = 4.215, p = 0.01, partial η2 = 0.426) improved continuously after the intervention. Based on this study, actionable and regular fundamental movement skill programs can be provided in the future as an effective way to achieve the healthy development goals of children with ASD.
Demographic Influences on Adult HPV Vaccination: Results from a Cross-Sectional Survey in Tennessee
Authors: Cernasev Alina , Oyedeji Oluwafemifola , Springer Cary M. , Hagemann Tracy M. , Hohmeier Kenneth C. , Kintziger Kristina W.
Published: July 2024

HPV is the most prevalent sexually transmitted infection in the U.S., with more than 80% of all Americans contracting it by age 45. Effective vaccines for HPV exist and were recently approved for adults aged 27–45 years, though uptake remains low in all age groups, particularly in Tennessee where 1089 cancers were attributed to HPV in 2020. Between 29 June and 17 August 2023, we conducted a cross-sectional survey to gain insights about the barriers and facilitators of HPV in 2011 adults aged 18 to 45 years in Tennessee. We developed our survey based on previous instruments to understand predictors of HPV vaccination in adults. Using descriptive statistics and bivariate and logistic regression analyses, we found higher vaccination rates in females, participants aged 18–38 years, participants with a high school education or higher, Hispanic or Latine individuals, and participants identifying as moderate or liberal. These insights highlight the need for public health interventions that consider demographic differences to successfully increase vaccination rates and reduce HPV-associated cancer risk.
Effectiveness of Virtual Reality on Rehabilitation of Chronic Non-Specific Low Back Pain Patients
Authors: Hussein Hisham , Atteya Mohamed , Kamel Ehab
Published: July 2024

Background: Virtual reality (VR) is used extensively for musculoskeletal conditions, but its efficacy in chronic low back pain still needs more investigation. Objective: To discuss the effectiveness of VR on selected outcomes in Chronic Non-Specific Low Back Pain (CNSLBP). Methods: Thirty-five patients with CNSLBP joined this study. Postural correction exercises using the TBed VR gaming system in addition to hamstring stretching were employed, and moist heat on the low back was applied. Pre- and post-intervention values of pain, ROM, function, and balance (overall stability index) were obtained using the numerical rating pain scale (NPRS), Oswestry Disability Index, back range of motion (BROM), and Biodex system. Satisfaction level on a 1–10 scale and the degree of commitment to the exercise sessions were assessed after the intervention. Results: The patients completed the intervention period and outcome measures sessions. Paired t-tests reported statistically significant improvements and high effect size in pain, ROM, function, and balance after the end of the treatment (p < 0.001, Cohen’s d > 0.69). The level of satisfaction was 9.25 ± 0.766, and the commitment to exercise sessions was high (98.75% attendance rate). Conclusions: Applying postural correction using TBed VR gaming in addition to heat and stretching may improve pain, range of motion, function, and balance in patients with chronic low back pain.
Development and Effects of Adult Nursing Education Programs Using Virtual Reality Simulations
Authors: Lee Eunju , Baek Gyuli
Published: July 2024

A virtual-reality-simulation-based nursing education program incorporating an information processing model helps nursing students develop their learned knowledge as nursing behavior and develop their ability to cope with complex clinical challenges. The purpose of this study is to develop a nursing education program using an immersive virtual reality simulation app for clinical situations based on an information processing model and identify the effects. A non-quantitative control group pretest–post-test design was employed. The programs were developed using the ADDIE model and an information processing model. In order to verify the effectiveness of the program, six adult nursing learning issues were taught to the experimental group over 6 weeks. The nursing education program in this study provides comprehensive experiential learning through advanced virtual simulation, significantly enhancing nursing students’ performance confidence, critical-thinking abilities, and problem-solving skills across a wide range of clinical scenarios. By repeatedly engaging with diverse learning topics related to adult nursing, this program not only equips students with essential practical skills but also contributes to the overall improvement of patient safety and the quality of medical care.
Association between Alcohol Use Disorder and Suicidal Ideation Using Propensity Score Matching in Chungcheongnam-do, South Korea
Authors: Yang Jeong-Min , Kim Jae-Hyun , Kim Min-Soo , Hong Ji-Sung , Gu Bon-Hee , Park Ju-Ho , Choi Young-Long , Lee Jung-Jae
Published: July 2024

Objectives: This study aimed to analyze the association between alcohol use disorder (AUD) and suicidal ideation (SI) in the general Korean population. Methods: The 2022 Mental Health Awareness Survey was collected from the Chungcheongnam-do Mental Health Welfare Center (CHMHC). Before Propensity Score Matching (PSM), 823 participants were included in this study. After 1:4 PSM, the 255 participants were analyzed using the chi-square test and matched conditional logistic regression. Results: The AUD group had higher odds of experiencing SI than the non-AUD (adjusted odds ratio [AOR]: 2.40, 95% confidence intervals [CI]: 1.10–5.22). Stratified matched conditional logistic regression showed that, among the female, <40 years and single group, the AUD group was more likely to experience SI compared with the non-AUD, respectively (AOR:3.53, 95% CI: 1.20–10.44/AOR:3.45, 95% CI: 1.03–11.55/AOR:4.83, 95% CI: 1.18–19.69). However, among the male, ≥40 years and married group, we discovered no association between AUD and SI. Conclusions: Through this study, we found a strong association between the AUD group and SI. This association was particularly strong among female, <40 years, and single groups. This study elucidates the relationship between AUD and SI in the Chungnam region, which had not been previously identified in Korea, and it is expected to serve as foundational data for reducing the high suicide rate in this region. However, due to the limitation of being a cross-sectional study, future longitudinal research is required.
Prevalence of Older Hospitalised Adults with Sustained Fractures after a Fall in Regional Australian Hospitals
Authors: Holden Emma , Devin Ruth , Bhattacharya Joyita , Waldie Frances , Watt Isabel , Wu Chiung-Jung (Jo)
Published: July 2024

Falls commonly occur in hospitals, particularly among older adults. Fractures in the older population can cause major morbidity, which can result in long hospital admissions and increased care costs. This study aimed to characterise the demographics of patients aged 65 years and over who fell in hospital and to determine the type of fractures they sustained. A descriptive study was undertaken to examine hospital data of older inpatients who had a fall during admission in two regional Queensland hospitals in Australia over a 2.5-year period. The prevalence of inpatient falls was 1.28%. Most falls were unwitnessed (77.34%) and they had an average of seven medical comorbidities. The mean age was 80.4 years and 63% were male. Women who fell were significantly older than men (p = 0.004). The mean length of stay of in-hospital fallers was 22.77 days and same admission mortality was 9.3%. Thirty-three fall events (3.8%) resulted in fractures, some with multiple injuries. The most common fracture was neck of femur, followed by rib, femur, and facial fractures. In conclusion, this study identifies the incidence of falls increased with age, most falls were unwitnessed, as well as provides evidence that patients with falls had multiple comorbidities and long hospital admissions. The data could be used to optimise fall prevention strategies and to refine post-fall assessment pathways.
Applied Hedge Algebra Approach with Multilingual Large Language Models to Extract Hidden Rules in Datasets for Improvement of Generative AI Applications
Authors: Pham Hai Van , Moore Philip
Published: July 2024

Generative AI applications have played an increasingly significant role in real-time tracking applications in many domains including, for example, healthcare, consultancy, dialog boxes (common types of window in a graphical user interface of operating systems), monitoring systems, and emergency response. This paper considers generative AI and presents an approach which combines hedge algebra and a multilingual large language model to find hidden rules in big data for ChatGPT. We present a novel method for extracting natural language knowledge from large datasets by leveraging fuzzy sets and hedge algebra to extract these rules, presented in meta data for ChatGPT and generative AI applications. The proposed model has been developed to minimize the computational and staff costs for medium-sized enterprises which are typically resource and time limited. The proposed model has been designed to automate question–response interactions for rules extracted from large data in a multiplicity of domains. The experimental results show that the proposed model performs well using datasets associated with specific domains in healthcare to validate the effectiveness of the proposed model. The ChatGPT application in case studies of healthcare is tested using datasets for English and Vietnamese languages. In comparative experimental testing, the proposed model outperformed the state of the art, achieving in the range of 96.70–97.50% performance using a heart dataset.
Evolution and Future of Serious Game Technology for Older Adults
Authors: Huang Xin , Ali Nazlena Mohamad , Sahrani Shafrida
Published: July 2024

Serious games play a key role in the medical field, particularly in enhancing cognitive abilities in the elderly. However, the sensory organs of the elderly decline over time, and the intervention effect of traditional serious games for older adults. The objective of this study is to identify the evolution and current problems of serious game technology for the elderly by using bibliometric analysis. We selected 319 relevant documents from 2013 to 2024 from the Web of Science (WOS) database. This study uses Publish or Perish (Windows GUl Edition) and VOSviewer (1.6.20) for performance analysis and scientific charting. We deeply analyze the early trends, emerging technologies, and publication trends, including citations and journals, subject areas, and regional and institutional. Here, we identified serious games for older adults rely heavily on visual presentation, often utilizing screens for screening, rehabilitation, and therapeutic interventions. This may cause further visual impairment in older adults who are experiencing visual decline. In addition, we proposed the combination of rich tactile feedback and external devices as one of the effective solutions to the current problems for future research.
An Analysis of the Development Factors of Rail Freight Transport in Thailand: A Structural Equation Modeling Approach
Authors: Buthphorm Oranicha , Sukhotu Vatcharapol , Hengsadeekul Thammanoon
Published: July 2024

The railway infrastructure projects in Thailand aim to shift transportation from roads to railways. This is crucial for transporting goods in emerging economies and increasing the demand for rail freight transport. However, several dynamic uncertainties hinder sustainable rail freight transport in Thailand. This study aims to identify the key factors and validate their effects on the success of the modal shift from roads to railways in Thailand. A total of 200 participants filled out a questionnaire delivered online and via postal service. The key factors were categorized into the following categories: the rail freight transport system, demand, and development factors in Thailand. The inter-relationship and connection of these factors were analyzed using SEM (structural equation modeling). The SEM results showed that all causal factors in the model had a positive influence on rail freight development in Thailand, which explained 98.3% of the variance in the factors influencing development. This study’s findings underscore the influential significance of rail performance, rail infrastructure, the legal framework, pricing, mode choice, and technology on the expansion of rail freight transport in Thailand. The rail freight transport system, rail performance, rail infrastructure, and Thailand’s rail infrastructure development strategy were significant direct predictors of rail freight expansion. An expansion of the rail freight transport system also leads to rail freight demand. The results of this study have positive implications for the government, railway practitioners, and policymakers to prioritize their focus on achieving rail freight transport as the national target.
Utilizing Biomolecule-Rich Citrus Fruit Waste as a Medium for the Eco-Friendly Preparation of Silver Nanoparticles with Antimicrobial Properties
Authors: Bhat Ramesa Shafi , Al-Dbass Abeer M. , Khayyat Arwa Ishaq A. , Al-Daihan Sooad
Published: July 2024

An ample amount of fruit waste is generated as agro-industrial waste, leading to significant nutritional, economic, and environmental challenges. Fruit peels are rich in many valuable bioactive compounds with the potential for developing nanoparticles. This study examined fresh juices of two citrus fruit peel wastes (Citrus sinensis: C. sinesis and Citrus limon: C. limon) for antioxidants and total protein. Then, we investigated their ability to produce silver nanoparticles, which were further analyzed for anti-microbial activity against thirteen pathogenic microbes. Both Citrus peel juices were rich in secondary metabolites. The formation of Ag nanoparticles was initially confirmed by UV-vis spectroscopy, with peaks at 400 nm for C. sinensis peel Ag nanoparticles and 430 nm for C. limon peel Ag nanoparticles. Further characterization was conducted using zeta sizer, zeta potential, Transmission Electron Microscopy (TEM), Scanning Electron Microscope (SEM), Energy-dispersive X-ray spectroscopy (EDX) and Fourier transform infrared (FTIR) spectroscopy. The antimicrobial activity was tested using the well diffusion method against 11 bacterial strains (five Gram-positive and six Gram-negative) and two fungal strains of Candida. TEM and SEM results revealed a spherical shape, with an average diameter of about 13 nm for C. sinensis and 21 nm for C. limon Ag. EDX confirmed the presence of silver in both nanoparticles. The FTIR spectrum of the extract indicated the presence of biomolecules, which facilitated the reduction and capping of the synthesized Ag nanoparticles. The prepared nanoparticles showed remarkable antimicrobial activity, but the nanoparticles from C. sinensis exhibited stronger antibacterial properties because of their smaller size. Citrus peel waste is a suitable medium for the eco-friendly production of silver nanoparticles.
Effect of Sex, Age and Temperature on the Functional Response of Macrolophus pygmaeus Ramber and Nesidiocoris tenuis Reuter (Heteroptera: Miridae) on Eggs of Tuta absoluta
Authors: Yiacoumi Eleni , Kouloussis Nikos A. , Koveos Dimitrios S.
Published: July 2024

The predatory mirids Macrolophus pygmaeus Ramber (Heteroptera: Miridae) and Nesidiocoris tenuis Reuter (Heteroptera: Miridae) are used for the biological control of Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) and other pests in tomato greenhouses. The functional response of 1-day-old (young) and 10-day-old (old) adult females and males of M. pygmaeus and N. tenuis on eggs of T. absoluta was determined on tomato at two temperatures (20 °C and 25 °C) and LD 16:8. Females of M. pygmaeus exhibited higher predation efficiency than males at both tested temperatures. Young M. pygmaeus females had a higher efficiency than old ones, whereas males had a low efficiency irrespective of age. The predation efficiency of N. tenuis was high (but lower than M. pygmaeus) in both young females and males, although old females had a higher efficiency than the respective males. Our results show that the two predatory species have different functional response characteristics to their prey depending mainly on sex and age, which may affect their role as biological control agents.
Population Growth Parameters of Scymnus nubilus Fed Single-Aphid Diets of Aphis fabae or Myzus persicae
Authors: Borges Isabel , Dury Guillaume J. , Soares António O.
Published: July 2024

Life tables are an important tool to forecast the performance of biological control agents used in pest management programs, and they are often assessed in terms of population growth. In the present study, the suitability of the aphids Aphis fabae Scopoli and Myzus persicae (Sulzer) for the ladybird predator Scymnus nubilus Mulsant was assessed for the first time. For this, we evaluated and compared the life history traits of immature individuals and adults of the predator fed single-aphid diets and the consequences of the single-aphid diets for the demographic parameters. Scymnus nubilus that were fed A. fabae were significantly more fecund and presented a shorter immature development time than those fed M. persicae. The predators fed A. fabae had a significantly higher net reproductive rate, an intrinsic and finite rate of increase, while their doubling time was significantly lower than that of those fed M. persicae. The aphid species used in this study are new additions to the essential prey list of the ladybird, with the predator presenting a better biological performance than that found on the previously known essential prey species.
The Potential Implications of Sex-Specific Differences in the Intestinal Bacteria of the Overwintering Wolf Spider Pardosa astrigera (Araneae: Lycosidae)
Authors: Li Ningkun , Yuan Quan , Qi Yaru , Wu Pengfeng , Cui Shuyan , Zheng Guo
Published: July 2024

Gut microbiota can promote the resistance of host arthropods to low-temperature stress. Female Pardosa astrigera have a lower anti-freeze compound level and weaker resistance to cold temperatures than the males in winter, which implies that their intestinal bacteria may be different during overwintering. This study primarily compared the intestinal bacterial communities between the two sexes of P. astrigera in a temperate region using 16S rRNA gene sequencing. Our findings indicated that the Chao1 and Shannon indices of intestinal bacteria in females were significantly higher than those in males, while the Simpson index in females was significantly lower than that in males. The male intestinal bacterial community was characterized by Proteobacteria and Actinobacteriota at the phylum level and by Pseudomonas and Rhodococcus at the genus level, with total relative abundances of 89.58% and 85.22%, respectively, which were also significantly higher than those in females, whose total relative abundances were 47.49% and 43.68%, respectively. In contrast, the total relative abundances of Bacteroidota and Firmicutes were significantly lower in males (4.26% and 4.75%, respectively) than in females (26.25% and 22.31%, respectively). Noteworthy divergences in bacterial communities were also found through an LEfSe analysis between females and males. Additionally, the results of the PICRUSt2 analysis showed that six out of eleven level-2 pathways related to key metabolic functions were significantly (or marginally significantly) higher in females than males, and five other level-2 pathways were significantly (or marginally significantly) lower in females than males. Our results imply that significant gender differences exist in intestinal bacterial communities of overwintering P. astrigera. We suggest that Pseudomonas versuta (belonging to Proteobacteria) and Rhodococcus erythropolis (belonging to Actinobacteriota) may have the potential to play key roles in overwintering P. astrigera.
Mealybug Population Dynamics: A Comparative Analysis of Sampling Methods for Saccharicoccus sacchari and Heliococcus summervillei in Sugarcane (Saccharum sp. Hybrids)
Authors: Xu Hang , Humpal Jacob A. , Wilson Bree A. L. , Ash Gavin J. , Powell Kevin S.
Published: July 2024

This research is focused on a comparative field-based study of the population dynamics and sampling methods of two mealybug species, Saccharicoccus sacchari (Cockerell, 1895) (Hemiptera: Coccomorpha, Pseudococcidae) and Heliococcus summervillei (Brookes, 1978) (Hemiptera: Coccomorpha, Pseudococcidae), in sugarcane (Saccharum sp. hybrids) (f. Poaceae) over consecutive growing seasons. The research monitored and compared the above- and belowground populations and seasonal abundance of these two mealybug species in sugarcane fields in Far North Queensland, with non-destructive sampling techniques of yellow sticky traps, pan traps, and stem traps, and destructive sampling of the whole leaf and whole plant. The results indicated that S. sacchari (n = 29,137) was more abundant and detected throughout the growing season, with population peaks in the mid-season, while H. summervillei (n = 2706) showed peaks of the early-season activity. S. sacchari is primarily located on sugarcane stems and roots, compared to H. summervillei, which is located on leaves and roots. The whole-leaf collection and stem trap were the most effective sampling techniques for quantification of H. summervillei and S. sacchari, respectively. This study enhanced the understanding of S. sacchari and the first-ever record of H. summervillei on sugarcane in Australia and will contribute to the development of more effective pest management strategies.
Systematic Review on the Role of IL-6 and IL-1β in Cardiovascular Diseases
Authors: Katkenov Nurlubek , Mukhatayev Zhussipbek , Kozhakhmetov Samat , Sailybayeva Aliya , Bekbossynova Makhabbat , Kushugulova Almagul
Published: July 2024

Cardiovascular diseases (CVDs) are a leading cause of global morbidity and mortality, significantly driven by chronic inflammation. Interleukin-6 (IL-6) and interleukin-1β (IL-1β) are critical inflammatory cytokines implicated in CVD progression. This systematic review evaluates the roles of IL-6 and IL-1β in CVDs by synthesizing data from relevant studies to understand their impact on cardiovascular outcomes and identify potential therapeutic interventions. A comprehensive literature search was conducted using PubMed and Embase, covering studies from January 2014 to December 2024. Inclusion criteria encompassed studies investigating IL-6 and/or IL-1β in CVDs, including human and relevant animal models, and reporting clinical outcomes, molecular mechanisms, or therapeutic interventions. Data extraction and quality assessment were performed independently by two reviewers. Our review included 12 studies focusing on the roles of IL-6 and IL-1β in various CVDs. Elevated IL-6 levels were significantly associated with peripheral artery disease, myocardial infarction, and heart failure, while IL-1β levels were linked to worse outcomes in coronary artery disease and heart failure. Meta-analyses indicated a significant association between higher IL-6 and IL-1β levels and increased risk of adverse cardiovascular events. These findings suggest that targeting IL-6 and IL-1β could offer promising therapeutic strategies for reducing inflammation and improving cardiovascular outcomes.
Non-Motor Symptoms in Primary Familial Brain Calcification
Authors: Bonato Giulia , Cimino Paola , Pistonesi Francesca , Salviati Leonardo , Bertolin Cinzia , Carecchio Miryam
Published: July 2024

Background/Objectives: Primary Familial Brain Calcification is a rare neurodegenerative disorder of adulthood characterized by calcium deposition in the basal ganglia and other brain areas; the main clinical manifestations include movement disorders, mainly parkinsonism. Non-motor symptoms are not well defined in PFBC. This work aims at defining the burden of non-motor symptoms in PFBC. Methods: A clinical, genetic and neuropsychological evaluation of a cohort of PFBC patients, COMPASS-31 scale administration. Results: A total of 50 PFBC patients were recruited; in 25, the genetic test was negative; 10 carried mutations in SLC20A2 gene, 8 in MYORG, 3 in PDGFB, 1 in PDGFRB, 2 in JAM2 (single mutations), and one test is still ongoing. The main motor manifestation was parkinsonism. Headache was reported in 26% of subjects (especially in PDGFB mutation carriers), anxiety or depression in 62%, psychosis or hallucinations in 10–12%, sleep disturbances in 34%; 14% of patients reported hyposmia, 32% constipation, and 34% urinary disturbances. A neuropsychological assessment revealed cognitive involvement in 56% (sparing memory functions, to some extent). The COMPASS-31 mean score was 20.6, with higher sub-scores in orthostatic intolerance and gastrointestinal problems. MYORG patients and subjects with cognitive decline tended to have higher scores and bladder involvement compared to other groups. Conclusions: The presence of non-motor symptoms is frequent in PFBC and should be systematically assessed to better meet patients’ needs.
A Design Optimization Study of Step/Scarf Composite Panel Repairs, Targeting the Maximum Strength and the Minimization of Material Removal
Authors: Psarras Spyridon , Giannoutsou Maria-Panagiota , Kostopoulos Vassilis
Published: July 2024

This study aimed to optimize the geometry of composite stepped repair patches, using a parametric algorithm to automate the process due to the complexity of the optimization problem and various factors affecting efficiency. More specifically, the algorithm initially calculates the equivalent strengths of the repaired laminate plate according to a max stress criterion, then calculates the dimensions of several elliptical repair patches, taking into account several design methods extracted from the literature. Next, it creates their finite element models and finally, the code conducts an assessment of the examined patch geometries, given specific user-defined criteria. In the end, the algorithm reaches a conclusion about the optimum patch among the designed ones. The algorithm has the potential to run for many different patch geometries. In the current research, five patch geometries were designed and modeled under uniaxial compressive loading at 0°, 45° and 90°. Overall, the code greatly facilitated the design and optimization process and constitutes a useful tool for future research. The results revealed that elliptical stepped patches can offer a near-optimum solution much more efficient than that of the conservative option of the circular patch, in terms of both strength and volume of healthy removed material.
Anodic Production and Characterization of Biomimetic Oxide Layers on Grade 4 Titanium for Medical Applications
Authors: Nowińska Delfina , Osak Patrycja , Maszybrocka Joanna , Łosiewicz Bożena
Published: July 2024

Biomaterials are the basis for the development of medicine because they allow safe contact with a living organism. The aim of this work was to produce innovative oxide layers with a microporous structure on the surface of commercially pure titanium Grade 4 (CpTi G4) and to characterize their properties as drug carriers. The anodization of the CpTi G4 subjected to mechanical grinding and electrochemical polishing was carried out in a solution of 1M ethylene glycol with the addition of 40 g of ammonium fluoride at a voltage of 20 V for 2, 18, 24, and 48 h at room temperature. It was found that the longer the anodization time, the greater the number of pores formed on the CpTi G4 surface as revealed using the FE-SEM method, and the greater the surface roughness determined in profilometric tests. As the anodizing time increases, the amount of the drug in the form of gentamicin sulfate incorporated into the resulting pores decreases. The most favorable drug release kinetics profile determined via UV–VIS absorption spectroscopy was found for the CpTi G4 anodized for 2 h.
Data Mining Paths for Standard Weekly Training Load in Sub-Elite Young Football Players: A Machine Learning Approach
Authors: Teixeira José E. , Encarnação Samuel , Branquinho Luís , Morgans Ryland , Afonso Pedro , Rocha João , Graça Francisco , Barbosa Tiago M. , Monteiro António M. , Ferraz Ricardo , Forte Pedro
Published: July 2024

The aim of this study was to test a machine learning (ML) model to predict high-intensity actions and body impacts during youth football training. Sixty under-15, -17, and -19 sub-elite Portuguese football players were monitored over a 6-week period. External training load data were collected from the target variables of accelerations (ACCs), decelerations (DECs), and dynamic stress load (DSL) using an 18 Hz global positioning system (GPS). Additionally, we monitored the perceived exertion and biological characteristics using total quality recovery (TQR), rating of perceived exertion (RPE), session RPE (sRPE), chronological age, maturation offset (MO), and age at peak height velocity (APHV). The ML model was computed by a feature selection process with a linear regression forecast and bootstrap method. The predictive analysis revealed that the players’ MO demonstrated varying degrees of effectiveness in predicting their DEC and ACC across different ranges of IQR. After predictive analysis, the following performance values were observed: DEC (x¯predicted = 41, β = 3.24, intercept = 37.0), lower IQR (IQRpredicted = 36.6, β = 3.24, intercept = 37.0), and upper IQR (IQRpredicted = 46 decelerations, β = 3.24, intercept = 37.0). The player’s MO also demonstrated the ability to predict their upper IQR (IQRpredicted = 51, β = 3.8, intercept = 40.62), lower IQR (IQRpredicted = 40, β = 3.8, intercept = 40.62), and ACC (x¯predicted = 46 accelerations, β = 3.8, intercept = 40.62). The ML model showed poor performance in predicting the players’ ACC and DEC using MO (MSE = 2.47–4.76; RMSE = 1.57–2.18: R2 = −0.78–0.02). Maturational concerns are prevalent in football performance and should be regularly checked, as the current ML model treated MO as the sole variable for ACC, DEC, and DSL. Applying ML models to assess automated tracking data can be an effective strategy, particularly in the context of forecasting peak ACC, DEC, and bodily effects in sub-elite youth football training.
Effects of Muscular Fatigue on Position Sense in Two Phases of the Menstrual Cycle
Authors: Roditi Elmina-Eleftheria , Tsatalas Themistoklis , Sakkas Giorgos K. , Koutedakis Yiannis , Giakas Giannis , Karatzaferi Christina
Published: July 2024

It is generally accepted that local muscular fatigue can negatively affect position sense. Interestingly, it has been proposed that in women, position sense and neuromuscular coordination may be affected by fluctuations of estrogen and progesterone levels. The aim of this study was to examine the possible effect of localized muscle fatigue on knee joint position sense at two phases of the menses: follicular and luteal. Twenty physically active females aged 19–30 years, with normal menses, volunteered for this study. An isokinetic dynamometer was used to evaluate proprioception and perform the fatigue protocol of the knee extensors and flexors. Knee proprioception at rest and after fatigue at three knee target angles (30°, 45°, 60°) was measured. A three-way ANOVA analysis with repeated measures was performed. The results showed that the main effect of fatigue was significant, but no main effect of the menstrual cycle phase was found. Additionally, a main effect was found for the target angle (more flexed target knee joint angles were associated with larger angular error deviations). In conclusion, localized muscle fatigue can significantly reduce the accuracy of active knee joint repositioning in both the luteal and the follicular menstrual phases in young, physically active healthy women.
Parameter Identification of Maritime Vessel Rudder PMSM Based on Extended Kalman Particle Filter Algorithm
Authors: Yuan Tianqing , Wang Tianli , Bai Jing , Fan Jingwen
Published: July 2024

To address the issue of system parameter variations during the operation of a maritime light vessel rudder permanent magnet synchronous motor (PMSM), an extended Kalman particle filter (EKPF) algorithm that combines a particle filter (PF) with an extended Kalman filter (EKF) is proposed in this paper. This approach enables the online identification of motor resistance and inductance. For highly nonlinear problems that are challenging for traditional methods such as Kalman filtering, this algorithm is typically a statistical and effective estimation method that usually yields good results. Firstly, a standard linear discrete parameter identification model is established for a PMSM. Secondly, the PF algorithm based on Bayesian state estimation as a foundation for subsequent research is derived. Thirdly, the advantages and limitations of the PF algorithm are analyzed, addressing issues such as sample degeneracy, by integrating it with the Kalman filtering algorithm. Specifically, the EKPF algorithm for online parameter identification is employed. Finally, the identification model within MATLAB/Simulink is constructed and the simulation studies are executed to ascertain the viability of our suggested algorithm. The outcomes from these simulations indicate that the proposed EKPF algorithm identifies resistance and inductance values both swiftly and precisely, markedly boosting the robustness and enhancing the control efficacy of the PMSM.