bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the ethical implications of the late discovery of adoptive and donor-insemination offspring status
Authors: Riley Helen
Published: July 2013

Some children adopted under the now discredited period of closed adoption were never told of their adoptive status until it was revealed to them in adulthood. Yet to date, this ‘late-discovery’ experience has received little research attention. Now a new generation of ‘late discoverers’ is emerging as a result of (heterosexual couple) donor insemination (DI) practices. This study of 25 late-discovery participants of either adoptive or (heterosexual couple) DI offspring status reveals ethical concerns particular to the lateness of discovery. Most of the participants were Australian, with the remainder from the UK, USA and Canada. All were asked to give an ‘open’ account of their experience, with four themes or suggestions provided on request. These accounts were added to those available in relevant publications. The analysis employed a hermeneutic phenomenological methodology and all accounts were analysed using an ethical perspective developed by Walker (2006 , 2007) . The main themes that emerged were: disrupted personal autonomy, betrayal of deep levels of trust and feelings of injustice and diminished self-worth. The lack of recognition of concerns particular to late discovery has resulted in late discoverers (i) feeling unable to regain a sense of personal control, (ii) significantly disrupted relationships with those closest to them and others, including community and institutions, and (iii) feelings of diminished value and self-worth.
Holding therapy in Britain: historical background, recent events, and ethical concerns
Authors: Mercer Jean
Published: July 2013

Holding therapy, an intervention often used in the treatment of foster and adopted children, has been rejected by professional groups on grounds of lack of evidentiary support and of potential harmfulness. Nevertheless, some British proponents have continued to advocate its use. Is this support brought about by the familiarity of concepts used in this treatment? This article reviews the history of related concepts and methods in Britain. It is concluded that a long history of British involvement with related ideas may have encouraged approval of holding therapy, but that ethical concerns argue against its use.
Toward a Godly Mode of Being: Virtue as Embodied Deification
Authors: Hamalis Perry T. , Papanikolaou Aristotle
Published: July 2013

Attention to virtue ethics in Eastern Christianity complicates the dominant narrative within the field by revealing new ways of conceptualizing classical problems in virtue theory, new insights into the dynamics of virtues’ development, as well as new contexts for applied virtue ethics. Human flourishing is understood as the progressive realization of theosis—a godly mode of being cultivated through liturgy and askesis, marked by the embodiment of the full range of virtues, and crowned by a radical love.
Corporeality and Askesis: Ethics and Bodily Practice in Gregory of Nyssa’s Theological Anthropology
Authors: Cadenhead Raphael
Published: July 2013

This article seeks to extend and refine Alastair MacIntyre’s moral theory of virtue ethics, by probing behind the Benedictine Rule—so fulsomely invoked at the end of After Virtue—to the ascetical theology of the noted, Eastern, ‘Cappadocian’ theologian of the fourth century: Gregory of Nyssa. I shall argue that Gregory’s vision of ascetical bodily practice complicates MacIntyre’s contemporary appropriation of virtue ethics. It does so by underscoring the diachronic, developmental character of personal ethical maturation—a theme which finds no expression in MacIntyre’s otherwise sophisticated account of ‘narrative’.
Liturgy and Ethics in Ancient Syriac Christianity: Two Paradigms
Authors: Harvey Susan Ashbrook
Published: July 2013

Early Syriac Christianity presents two notable paradigms for understanding liturgy as a means for the ethical formation of the congregation. Ephrem the Syrian (d. 373) in his hymns for the Nativity vigil, and Jacob of Sarug (d. 521) in his verse homilies, each addressed their congregations in ways that utilized ritual participation in the liturgy for ethical and moral cultivation. Ephrem sought to instill his congregation with a biblical and theological understanding of the Nativity that would yield ethical enactment in the world. Jacob worked to use the experience of liturgical participation to mold the Christian’s moral disposition. For both, God’s salvation enabled the healing of the human condition in its various dimensions: physical, social, ecclesial. Liturgy as disciplined ritual activity provided the tools by which their congregations could learn, experience, and enact that healing.
Evagrius Ponticus on Being Good in God and Christ
Authors: Konstantinovsky Julia
Published: July 2013

Can moral theories of the kind Evagrius Ponticus upheld be useful today? Is his ethics one of many other virtue ethical theories or is it something else entirely? I argue that Evagrius’s theory of virtue is an instance of traditional Christian moral theory. Moreover, as a Christian theory, Evagrius’s moral system stands apart from non-Christian moral theories, virtue, consequentialist and deontological. I further maintain that his morality is robust, because it is able to undercut some of the strongest critiques generally made against moral theories: those of inconsistency and selfishness. It is also successful, because it produced fertile cultural movements that continue at present. I conclude, however, that its theistic, kenotic and inverted nature presents a direct challenge to ethical assumptions of today.
Virtue Ethics: St Maximos the Confessor and Aquinas Compared
Authors: Louth Andrew
Published: July 2013

Traditionally Christian ethical reflection has taken the form of what is called nowadays ‘virtue ethics’. This article compares the approach to virtue ethics in the Byzantine thinker, Maximos the Confessor, and the Western thinker, Thomas Aquinas. They both share the heritage of Plato and Aristotle. Maximos develops a concern for the virtues that is practical and ascetic; although he recognizes and uses the traditional classical terminology, he prefers a new Christian terminology, based more directly on the Scriptures. In contrast, Aquinas accepts and uses the classical terminology, adding to it Christian ‘theological’ virtues. His concern is much less directly practical than Maximos, and more directed to the kind of society in which the virtues can flourish. This contrast between the ascetical and political (which should not be overdrawn) is manifest in Dumitru Stăniloae and Josef Pieper, introduced as modern interpreters of Maximos and Thomas respectively.
The Iconicity of Priesthood: Male Bodies or Embodied Virtue?
Authors: McDowell Maria Gwyn
Published: July 2013

Late-ancient theologies of the priesthood frame its tasks, virtues and metaphorical relationships around its chief task: encouraging a common life of theosis as embodied virtue. Metaphorical relationships are used to evoke the manner in which, and the virtue with which, priestly tasks are to be practiced. In the priest, we hope to see an icon of the deified humanity to which all are called. This theological structuring allows the participation of women in the sacramental priesthood. Modern Orthodox arguments, in their efforts to defend an exclusively male priesthood, subvert this structure. The language of relationship and virtue is used to define the priesthood according to specifically gendered tasks. This theology results in, and derives from, a reduced view of the full personhood of women and the breadth of the priestly task, undermining the priest’s role of embodying and encouraging all the virtues of the deified life.
The Synthetic Unity of Virtue and Epistemic Goods in Maximus the Confessor
Authors: Aquino Frederick D.
Published: July 2013

In this essay, I show how the virtues, for Maximus the Confessor, contribute to the formation of a positive orientation toward (a deep and abiding desire for) the relevant epistemic goods (e.g., contemplation of God in and through nature, illumination of divine truths, wisdom, and experiential knowledge of God). The first section offers a brief overview of how three character-based virtue epistemologies envision the role of the intellectual virtues in the cognitive life. The second section draws attention to Maximus’s understanding of the relationship between the virtues and the relevant epistemic goods. The third section argues that a relationship of this sort entails a seamless connection (though without confusion) between the practical and contemplative aspects of deiform existence. The fourth section clarifies how select virtues foster within the self a praiseworthy desire for the relevant epistemic goods.
Book Review: The Technological State in Indonesia: The Co-constitution of High Technology and Authoritarian Politics
Authors: Abraham Itty
Published: July 2013

SULFIKAR AMIR (2013), The Technological State in Indonesia: The Co-constitution of High Technology and Authoritarian Politics. London and New York: Routledge. xviii, 190 pp. Hardcover.
Oxidation and ubiquitination in neurodegeneration
Authors: Riederer Beat M , Leuba Geneviève , ElHajj Zeinab
Published: July 2013

It is widely accepted that protein oxidation is involved in a variety of diseases, including neurodegenerative diseases. Especially during aging, a reduction in anti-oxidant defence mechanisms leads to an increased formation of free radical oxygen species and consequently results in a damage of proteins, including mitochondrial and synaptic ones. Even those proteins involved in repair and protein clearance via the ubiquitin proteasome and lysosomal system are subject to damage and show a reduced function. Here, we will discuss a variety of mechanisms and provide examples where cognition is affected and where repair mechanisms are no longer sufficient to compensate for a dysfunction of damaged proteins or even may become toxic. Next to physiological deficits, an accumulation of deficient proteins in aggresomes may occur and result in a formation of pathological hallmark structures typical for aging and disease. A major challenge is how to prevent aberrant oxidation, given that oxidation plays an essential role in aging and neurodegenerative diseases. Particularly interesting are the possibilities to reduce the formation of radical oxygen species leading to a dysfunction of protein repair and protein clearance, or to a formation of toxic byproducts accelerating neurodegeneration.
Follicle growth, ovulation, and luteal formation in primates and rodents: A comparative perspective
Authors: Chaffin Charles L , VandeVoort Catherine A
Published: July 2013

Ovarian function has a great deal of functional overlap between species; antral follicles grow in response to FSH, ovulation involves proteolysis, and the steroidogenic pathway is largely the same. However, embedded in these similarities are important differences that reflect the evolutionary and natural history of species and may focus future research into these critical areas. This review compares ovarian function of rats and mice with primates, focusing on estradiol and follicle growth, steroidogenesis and rupture during the periovulatory interval, and the formation of a functional corpus luteum, drawing the conclusion that careful comparison of species yields more functional information about both than studying them in isolation.
Molecular basis of organ fibrosis: Potential therapeutic approaches
Authors: Ghosh Asish K , Quaggin Susan E , Vaughan Douglas E
Published: July 2013

Fibrosis, a non-physiological wound healing in multiple organs, is associated with end-stage pathological symptoms of a wide variety of vascular injury and inflammation related diseases. In response to chemical, immunological and physical insults, the body’s defense system and matrix synthetic machinery respond to healing the wound and maintain tissue homeostasis. However, uncontrolled wound healing leads to scarring or fibrosis, a pathological condition characterized by excessive synthesis and accumulation of extracellular matrix proteins, loss of tissue homeostasis and organ failure. Understanding the actual cause of pathological wound healing and identification of igniter(s) of fibrogenesis would be helpful to design novel therapeutic approaches to control pathological wound healing and to prevent fibrosis related morbidity and mortality. In this article, we review the significance of a few key cytokines (TGF-β, IFN-γ, IL-10) transcriptional activators (Sp1, Egr-1, Smad3), repressors (Smad7, Fli-1, PPAR-γ, p53, Klotho) and epigenetic modulators (acetyltransferase, methyltransferases, deacetylases, microRNAs) involved in major matrix protein collagen synthesis under pathological stage of wound healing, and the potentiality of these regulators as therapeutic targets for fibrosis treatment. The significance of endothelial to mesenchymal transition (EndMT) and senescence, two newly emerged fields in fibrosis research, has also been discussed.
Recent advances in the morphological and functional heterogeneity of the biliary epithelium
Authors: Han Yuyan , Glaser Shannon , Meng Fanyin , Francis Heather , Marzioni Marco , McDaniel Kelly , Alvaro Domenico , Venter Julie , Carpino Guido , Onori Paolo , Gaudio Eugenio , Alpini Gianfranco , Franchitto Antonio
Published: July 2013

This review focuses on the recent advances related to the heterogeneity of different-sized bile ducts with regard to the morphological and phenotypical characteristics, and the differential secretory, apoptotic and proliferative responses of small and large cholangiocytes to gastrointestinal hormones/peptides, neuropeptides and toxins. We describe several in vivo and in vitro models used for evaluating biliary heterogeneity. Subsequently, we discuss the heterogeneous proliferative and apoptotic responses of small and large cholangiocytes to liver injury and the mechanisms regulating the differentiation of small into large (more differentiated) cholangiocytes. Following a discussion on the heterogeneity of stem/progenitor cells in the biliary epithelium, we outline the heterogeneity of bile ducts in human cholangiopathies. After a summary section, we discuss the future perspectives that will further advance the field of the functional heterogeneity of the biliary epithelium.
SNVHMM: predicting single nucleotide variants from next generation sequencing
Authors: Bian Jiawen , Liu Chenglin , Wang Hongyan , Xing Jing , Kachroo Priyanka , Zhou Xiaobo
Published: July 2013

Background

The rapid development of next generation sequencing (NGS) technology provides a novel avenue for genomic exploration and research. Single nucleotide variants (SNVs) inferred from next generation sequencing are expected to reveal gene mutations in cancer. However, NGS has lower sequence coverage and poor SNVs detection capability in the regulatory regions of the genome. Post probabilistic based methods are efficient for detection of SNVs in high coverage regions or sequencing data with high depth. However, for data with low sequencing depth, the efficiency of such algorithms remains poor and needs to be improved.

Results

A new tool SNVHMM basing on a discrete hidden Markov model (HMM) was developed to infer the genotype for each position on the genome. We incorporated the mapping quality of each read and the corresponding base quality on the reads into the emission probability of HMM. The context information of the whole observation as well as its confidence were completely utilized to infer the genotype for each position on the genome in study. Therefore, more probability power can be gained over the Bayes based methods, which is very useful for SNVs detection for data with low sequencing depth. Moreover, our model was verified by testing against two sets of lobular breast tumor and Myelodysplastic Syndromes (MDS) data each. Comparing against a recently published SNVs calling algorithm SNVMix2, our model improved the performance of SNVMix2 largely when the sequencing depth is low and also outperformed SNVMix2 when SNVMix2 is well trained by large datasets.

Conclusions

SNVHMM can detect SNVs from NGS cancer data efficiently even if the sequence depth is very low. The training data size can be very small for SNVHMM to work. SNVHMM incorporated the base quality and mapping quality of all observed bases and reads, and also provides the option for users to choose the confidence of the observation for SNVs prediction.
Rationale and design of the Sodium Lowering In Dialysate (SoLID) trial: a randomised controlled trial of low versus standard dialysate sodium concentration during hemodialysis for regression of left ventricular mass
Authors: Dunlop Joanna Leigh , Vandal Alain Charles , de Zoysa Janak Rashme , Gabriel Ruvin Sampath , Haloob Imad Adbi , Hood Christopher John , Matheson Philip James , McGregor David Owen Ross , Rabindranath Kannaiyan Samuel , Semple David John , Marshall Mark Roger
Published: July 2013

Background

The current literature recognises that left ventricular hypertrophy makes a key contribution to the high rate of premature cardiovascular mortality in dialysis patients. Determining how we might intervene to ameliorate left ventricular hypertrophy in dialysis populations has become a research priority. Reducing sodium exposure through lower dialysate sodium may be a promising intervention in this regard. However there is clinical equipoise around this intervention because the benefit has not yet been demonstrated in a robust prospective clinical trial, and several observational studies have suggested sodium lowering interventions may be deleterious in some dialysis patients.

Methods/design

The Sodium Lowering in Dialysate (SoLID) study is funded by the Health Research Council of New Zealand. It is a multi-centre, prospective, randomised, single-blind (outcomes assessor), controlled parallel assignment 3-year clinical trial. The SoLID study is designed to study what impact low dialysate sodium has upon cardiovascular risk in dialysis patients. The study intends to enrol 118 home hemodialysis patients from 6 sites in New Zealand over 24 months and follow up each participant over 12 months. Key exclusion criteria are: patients who dialyse more frequently than 3.5 times per week, pre-dialysis serum sodium of <135 mM, and maintenance hemodiafiltration. In addition, some medical conditions, treatments or participation in other dialysis trials, which contraindicate the SoLID study intervention or confound its effects, will be exclusion criteria. The intervention and control groups will be dialysed using dialysate sodium 135 mM and 140 mM respectively, for 12 months. The primary outcome measure is left ventricular mass index, as measured by cardiac magnetic resonance imaging, after 12 months of intervention. Eleven or more secondary outcomes will be studied in an attempt to better understand the physiologic and clinical mechanisms by which lower dialysate sodium alters the primary end point.

Discussion

The SoLID study is designed to clarify the effect of low dialysate sodium upon the cardiovascular outcomes of dialysis patients. The study results will provide much needed information about the efficacy of a cost effective, economically sustainable solution to a condition which is curtailing the lives of so many dialysis patients.

Trial registration

Australian and New Zealand Clinical Trials Registry number:ACTRN12611000975998
Efficacy and cost-effectiveness of a web-based and mobile stress-management intervention for employees: design of a randomized controlled trial
Authors: Heber Elena , Ebert David Daniel , Lehr Dirk , Nobis Stephanie , Berking Matthias , Riper Heleen
Published: July 2013

Background

Work-related stress is associated with a variety of mental and emotional problems and can lead to substantial economic costs due to lost productivity, absenteeism or the inability to work. There is a considerable amount of evidence on the effectiveness of traditional face-to-face stress-management interventions for employees; however, they are often costly, time-consuming, and characterized by a high access threshold. Web-based interventions may overcome some of these problems yet the evidence in this field is scarce. This paper describes the protocol for a study that will examine the efficacy and cost-effectiveness of a web-based guided stress-management training which is based on problem solving and emotion regulation and aimed at reducing stress in adult employees.

Methods

The study will target stressed employees aged 18 and older. A randomized controlled trial (RCT) design will be applied. Based on a power calculation of d=.35 (1-β of 80%, α = .05), 264 participants will be recruited and randomly assigned to either the intervention group or a six-month waitlist control group. Inclusion criteria include an elevated stress level (Cohen’s Perceived Stress Scale-10 ≥ 22) and current employment. Exclusion criteria include risk of suicide or previously diagnosed psychosis or dissociative symptoms. The primary outcome will be perceived stress, and secondary outcomes include depression and anxiety. Data will be collected at baseline and seven weeks and six months after randomization. An extended follow up at 12 months is planned for the intervention group. Moreover, a cost-effectiveness analysis will be conducted from a societal perspective and will include both direct and indirect health care costs. Data will be analyzed on an intention-to-treat basis and per protocol.

Discussion

The substantial negative consequences of work-related stress emphasize the necessity for effective stress-management trainings. If the proposed internet intervention proves to be (cost-) effective, a preventative, economical stress-management tool will be conceivable. The strengths and limitations of the present study are discussed.

Trial registration

German Register of Clinical Studies (DRKS): DRKS00004749
Artesunate versus quinine in the treatment of severe imported malaria: comparative analysis of adverse events focussing on delayed haemolysis
Authors: Rolling Thierry , Wichmann Dominic , Schmiedel Stefan , Burchard Gerd D , Kluge Stefan , Cramer Jakob P
Published: July 2013

Background

Severe malaria is a potentially life-threatening infectious disease. It has been conclusively shown that artesunate compared to quinine is superior in antiparasitic efficacy and in lowering mortality showing a better short-term safety profile. Regarding longer-term effects, reports of delayed haemolysis after parenteral artesunate for severe malaria in returning travellers have been published recently. So far, delayed haemolysis has not been described after the use of parenteral quinine.

Methods

In this retrospective study, all patients treated for severe malaria at the University Medical Centre Hamburg-Eppendorf were included between 2006 and 2012. The primary endpoint was the proportion of delayed haemolysis in patients treated with quinine versus those who received artesunate. As secondary endpoint, the proportion of any adverse event was assessed.

Results

A total of 36 patients with severe malaria were included in the analysis. Of these, 16 patients contributed sufficient data to assess the endpoint delayed haemolysis. Twelve were treated primarily with intravenous quinine – with four patients having received intrarectal artesunate as an adjunct treatment – and five patients were treated primarily with artesunate. Five cases of delayed haemolysis could be detected – two in patients treated with quinine and intrarectal artesunate and three in patients treated with artesunate. No case of delayed haemolysis was detected in patients treated with quinine alone.

While adverse events observed in patients treated with artesunate were limited to delayed haemolysis (three patients, 60%) and temporary deterioration in renal function (three patients, 60%), patients treated with quinine showed a more diverse picture of side effects with 22 patients (71%) experiencing at least one adverse event. The most common adverse events after quinine were hearing disturbances (12 patients, 37%), hypoglycaemia (10 patients, 32%) and cardiotoxicity (three patients, 14%).

Conclusions

This study provides further evidence on delayed haemolysis after artesunate and underlines the importance of a standardized follow-up of patients treated with artesunate for severe malaria.
Role of moesin in hyaluronan induced cell migration in glioblastoma multiforme
Authors: DeSouza Leroi V , Matta Ajay , Karim Zia , Mukherjee Joydeep , Wang X Simon , Krakovska Olga , Zadeh Gelareh , Guha Abhijit , Siu KW Michael
Published: July 2013

Background

A major barrier to effective treatment of glioblastoma multiforme (GBM) is the invasion of glioma cells into the brain parenchyma rendering local therapies such as surgery and radiation therapy ineffective. GBM patients with such highly invasive and infiltrative tumors have poor prognosis with a median survival time of only about a year. However, the mechanisms leading to increased cell migration, invasion and diffused behavior of glioma cells are still poorly understood.

Methods

In the current study, we applied quantitative proteomics for the identification of differentially expressed proteins in GBMs as compared to non-malignant brain tissues.

Results

Our study led to the identification of 23 proteins showing overexpression in GBM; these include membrane proteins, moesin and CD44. The results were verified using Western blotting and immunohistochemistry in independent set of GBM and non-malignant brain tissues. Both GBM tissues and glioma cell lines (U87 / U373) demonstrated membranous expression of moesin and CD44, as revealed by immunohistochemistry and immunofluorescence, respectively. Notably, glioma cells transfected with moesin siRNA displayed reduced migration and invasion on treatment with hyaluronan (HA), an important component of the extracellular matrix in GBM. CD44, a transmembrane glycoprotein, acts as a major receptor for hyaluronan (HA). Using co-immunoprecipitation assays, we further demonstrated that moesin interacts with CD44 in glioma cells only after treatment with HA; this implicates a novel role of moesin in HA-CD44 signaling in gliomas.

Conclusions

Our results suggest that development of inhibitors which interfere with CD44-moesin interactions may open a new avenue in the future to mitigate cellular migration in gliomas.
Effect of chain architecture on the compression behavior of nanoscale polyethylene particles
Authors: Wu Jianyang , He Jianying , Odegard Gregory M , Zhang Zhiliang
Published: July 2013

Polymeric particles with controlled internal molecular architectures play an important role as constituents in many composite materials for a number of emerging applications. In this study, classical molecular dynamics techniques are employed to predict the effect of chain architecture on the compression behavior of nanoscale polyethylene particles subjected to simulated flat-punch testing. Cross-linked, branched, and linear polyethylene chain architectures are each studied in the simulations. Results indicate that chain architecture has a significant influence on the mechanical properties of polyethylene nanoparticles, with the network configuration exhibiting higher compressive strengths than the branched and linear architectures. These findings are verified with simulations of bulk polyethylene. The compressive stress versus strain profiles of particles show four distinct regimes, differing with that of experimental micron-sized particles. The results of this study indicate that the mechanical response of polyethylene nanoparticles can be custom-tailored for specific applications by changing the molecular architecture.
Selective reporting - a half signalling load algorithm for distributed sensing
Authors: Gameiro Atílio , Ribeiro Carlos , Quaresma José
Published: July 2013

Spectrum sensing is a powerful tool of the cognitive cycle to help circumvent the apparent spectrum scarcity faced by wireless transmission systems. To overcome the challenging issues faced by the localized sensing, multiple cognitive radios can cooperate to explore the multiuser diversity and generate a more reliable decision on the presence of a signal in the frequencies of interest. In such a cooperative sensing scenario, a common reporting channel is needed for the transmission of the information of each element. As the number of elements that participate in the sensing operation increases, so does the bandwidth demanded for the reporting channel, quickly becoming the limiting factor in this scenario. To tackle the issue of reducing the sensing report overhead, this paper introduces a new cooperative sensing scheme that introduces silence periods in the reporting and, relying on information theory principles, explores the information present in these periods to reduce by 50% the sensing reporting overhead while maintaining the same performance of standard reporting schemes. Numerical and experimental results confirm the theoretical analysis and show the predicted reduction in reporting overhead and performance preservation.
Physiological adaptations of Saccharomyces cerevisiae evolved for improved butanol tolerance
Authors: Ghiaci Payam , Norbeck Joakim , Larsson Christer
Published: July 2013

Background

Butanol is a chemical with potential uses as biofuel and solvent, which can be produced by microbial fermentation. However, the end product toxicity is one of the main obstacles for developing the production process irrespective of the choice of production organism. The long-term goal of the present project is to produce 2-butanol inSaccharomyces cerevisiae. Therefore, unraveling the toxicity mechanisms of solvents such as butanol and understanding the mechanisms by which tolerant strains ofS. cerevisiaeadapt to them would be an important contribution to the development of a bio-based butanol production process.

Results

A butanol tolerantS. cerevisiaewas achieved through a series of sequential batch cultures with gradual increase of 2-butanol concentration. The final mutant (JBA-mut) tolerates all different alcohols tested at higher concentrations compared to the wild type (JBA-wt). Proteomics analysis of the two strains grown under mild butanol-stress revealed 46 proteins changing their expression by more than 1.5-fold inJBA-mut, 34 of which were upregulated. Strikingly, 21 out of the 34 upregulated proteins were predicted constituents of mitochondria. Among the non-mitochondrial up-regulated proteins, the minor isoform of Glycerol-3-phosphatase (Gpp2) was the most notable, since it was the only tested protein whose overexpression was found to confer butanol tolerance.

Conclusion

The study demonstrates several differences between the butanol tolerant mutant and the wild type. Upregulation of proteins involved in the mitochondrial ATP synthesizing machinery constituents and glycerol biosynthesis seem to be beneficial for a successful adaptation of yeast cells to butanol stress.
Experimental investigation on the ultimate strength of partially infilled and steel-braced reinforced concrete frames
Authors: Damodar Dubey Shailendra Kumar , Kute Sunil Y
Published: July 2013

Infilled walls are normally considered as nonstructural elements. However, these walls are effective in carrying lateral loads. In this regard, an experimental investigation was planned and conducted to study the effect of braced and partially concrete-infilled, reinforced concrete (RC) frames in comparison to the bare frames. All these frames were tested up to collapse and subjected to only horizontal loads to obtain an effective and possible solution for soft story. In comparison to bare RC frames, partially infilled frames have more lateral load capacity. Central bracing is more effective than that of corner bracing. For the same load, braced and partially infilled frames deflected significantly less than that of the bare frames. Based on experimental observations, a mathematical model has been proposed to calculate theoretical ultimate load for braced and partially infilled RC frames.
Abdominal vascular emergencies: US and CT assessment
Authors: Genovese Eugenio Annibale , Fonio Paolo , Floridi Chiara , Macchi Monica , Maccaferri Anna , Ianora Antonio Amato Stabile , Cagini Lucio , Carrafiello Gianpaolo
Published: July 2013

Acute vascular emergencies can arise from direct traumatic injury to the vessel or be spontaneous (non-traumatic).

The vascular injuries can also be divided into two categories: arteial injury and venous injury.

Most of them are life-treatening emergencies, sice they may cause an important ipovolemic shock or severe ischemia in their end organ and require prompt diagnosis and treatment.

In the different clinical scenarios, the correct diagnostic approach to vascular injuries isn’t firmly established and advantages of one imaging technique over the other are not obvious.

Ultrasound (US) is an easy accessible, safe and non-invasive diagnostic modality but Computed Tomography (CT) with multiphasic imaging study is an accurate modality to evaluate the abdominal vascular injuries therefore can be considered the primary imaging modality in vascular emergencies.

The aim of this review article is to illustrate the different imaging options for the diagnosis of abdominal vascular emergencies, including traumatic and non traumatic vessel injuries, focusing of US and CT modalities.
Contrast enhanced ultrasound (CEUS) in blunt abdominal trauma
Authors: Cagini Lucio , Gravante Sabrina , Malaspina Corrado Maria , Cesarano Elviro , Giganti Melchiorre , Rebonato Alberto , Fonio Paolo , Scialpi Michele
Published: July 2013

In the assessment of polytrauma patient, an accurate diagnostic study protocol with high sensitivity and specificity is necessary. Computed Tomography (CT) is the standard reference in the emergency for evaluating the patients with abdominal trauma. Ultrasonography (US) has a high sensitivity in detecting free fluid in the peritoneum, but it does not show as much sensitivity for traumatic parenchymal lesions. The use of Contrast-Enhanced Ultrasound (CEUS) improves the accuracy of the method in the diagnosis and assessment of the extent of parenchymal lesions. Although the CEUS is not feasible as a method of first level in the diagnosis and management of the polytrauma patient, it can be used in the follow-up of traumatic injuries of abdominal parenchymal organs (liver, spleen and kidneys), especially in young people or children.
Effects of surface energies of biomimetic materials for stone conservation on reinforcement properties
Authors: Liu Qiang , Liu Qingju , Zhang Jin , Zhu Zhongqi , Zhang Bingjian
Published: July 2013

The conservation of historic stone buildings and sculptures is recently receiving growing attention and becoming a focus of research. However, it is difficult to estimate the effects of stone conservation, especially consolidating treatments. In this work, some biomimetic materials for stone conservation were prepared. The interaction between the protective materials and stone substrate was researched by testing their surface energy. Some thermodynamic parameters, including interfacial tension (γ

fm
) and work of adhesion (W

a
), were calculated. The effects of interaction between the materials and substrate on reinforcement properties were investigated. The results show that the strength of materials’ adhesion to a solid surface could be better estimated from the value of W

a
/γ

fm
. It is helpful not only to indirectly determine the mechanical properties of the reinforcing agent but also to design some materials for stone conservation on a purpose not on a rather hit-or-miss basis.
Optimization of macroelement concentrations, pH and osmolarity for triacylglycerol accumulation in Rhodococcus opacus strain PD630
Authors: Janßen Helge Jans , Ibrahim Mohammad H A , Bröker Daniel , Steinbüchel Alexander
Published: July 2013

The refinement of biodiesel or renewable diesel from bacterial lipids has a great potential to make a contribution for energy production in the future. This study provides new data concerning suitable nutrient concentrations for cultivation of the Gram-positive Rhodococcus opacus PD630, which is able to accumulate large amounts of lipids during nitrogen limitation. Enhanced concentrations of magnesium have been shown to increase the final optical density and the lipid content of the cells. Elevated phosphate concentrations slowed down the onset of the accumulation phase, without a clear effect on the final optical density and the cell’s lipid content. A robust growth of R. opacus was possible in the presence of ammonium concentrations of up to 1.4 g l-1 and sucrose concentrations of up to 240 g l-1, with an optimum regarding growth and lipid storage observed in the range of 0.2 to 0.4 g l-1 ammonium and 20 to 40 g l-1 sucrose, respectively. Moreover, R. opacus showed tolerance to high salt concentrations.
Controlled root targeted delivery of fertilizer using an ionically crosslinked carboxymethyl cellulose hydrogel matrix
Authors: Davidson Drew W , Verma Mohit S , Gu Frank X
Published: July 2013

Aims

The recent increases in food prices caused by the corresponding increases in fertilizer costs have highlighted the demand for reducing the overuse of fertilizers in industrial agriculture. There has been increasing interest in developing plant root-targeted delivery (RTD) of fertilizers in order to address the problem of inefficient fertilizer use. The aim of this study is to develop a low cost controlled release device to deliver fertilizers to plant roots and thereby increase fertilizer use efficiency.

Methods

The Root Targeted Delivery Vehicle (RTDV) is formed by dissolving Carboxymethyl Cellulose (CMC) chains in water, mixing it with liquid fertilizer and crosslinking using iron and calcium salts. Basic measurements quantifying nutrient release and green house growth trials were carried out to evaluate fertilizer use efficiency on wheat growing in nutrient depleted soil media.

Results

Growing wheat in nutrient depleted media showed that the RTDV permits a 78% reduction in the amount of fertilizer needed to achieve similar levels of plant yield in these conditions. Quantifying the losses associated with the RTDV synthesis showed that optimizing manufacturing could possibly increase this value as high as 94%. Furthermore, the delivery device showed a similar lifetime in soil to the plant’s growth cycle, delivering fertilizer over the course of the plant’s growth before removal from soil by degradation.

Conclusions

These results illustrate the importance of fertilizer delivery in facilitating absorption and may have potential to vastly increase the use efficiency of fertilizers in soil, resulting in a significant reduction of costs and environmental damage. With more in depth study to quantify the fertilizer release and refine the device, there is great potential for the use of the RTDV as an effective means to increase fertilizer use efficiency in agriculture.
Stochastic extension of cellular manufacturing systems: a queuing-based analysis
Authors: Fardis Fatemeh , Zandi Afagh , Ghezavati Vahidreza
Published: July 2013

Clustering parts and machines into part families and machine cells is a major decision in the design of cellular manufacturing systems which is defined as cell formation. This paper presents a non-linear mixed integer programming model to design cellular manufacturing systems which assumes that the arrival rate of parts into cells and machine service rate are stochastic parameters and described by exponential distribution. Uncertain situations may create a queue behind each machine; therefore, we will consider the average waiting time of parts behind each machine in order to have an efficient system. The objective function will minimize summation of idleness cost of machines, sub-contracting cost for exceptional parts, non-utilizing machine cost, and holding cost of parts in the cells. Finally, the linearized model will be solved by the Cplex solver of GAMS, and sensitivity analysis will be performed to illustrate the effectiveness of the parameters.
Hypoxia preconditioned bone marrow mesenchymal stem cells promote liver regeneration in a rat massive hepatectomy model
Authors: Yu Jun , Yin Shengyong , Zhang Wu , Gao Feng , Liu Yuanxing , Chen Zhiyun , Zhang Min , He Jiangjuan , Zheng Shusen
Published: July 2013

Introduction

Bone marrow mesenchymal stem cells (BMMSCs) have been reported to facilitate liver regeneration after toxic injuries. However, the effect of BMMSCs on liver regeneration after massive hepatectomy is barely studied. Here we explored whether infusion of BMMSCs promotes liver regeneration in a rat massive hepatectomy model.

Methods

Hypoxia preconditioning was achieved by culturing BMMSCs under a hypoxia environment. Then 85% hepatectomy was performed and hypoxia or normoxia preconditioned BMMSCs were infused into the portal vein. A group of rats received vascular endothelial growth factor (VEGF) neutralizing antibody perioperatively, and underwent 85% hepatectomy and a subsequent infusion of hypoxia preconditioned BMMSCs to verify the role of VEGF in the effects of BMMSCs on liver regeneration. Liver samples were collected and liver regeneration was evaluated postoperatively.

Results

Hypoxia preconditioning enhanced the expression of VEGF in BMMSCs in vitro. Infusion of BMMSCs promoted proliferation of hepatocytes, reflected by elevated cyclin D1 expression and proliferating cell nuclear antigen-positive hepatocytes. However, BMMSC infusion did not improve the serum albumin level, liver weight/body weight ratio, and survival after operation. Infusion of hypoxia preconditioned BMMSCs significantly elevated cyclin D1, proliferating cell nuclear antigen-positive hepatocytes, liver weight/body weight ratio, and survival compared with normoxia preconditioned BMMSCs, accompanied by an increased serum albumin level. The level of VEGF in liver homogenate was much higher in hypoxia preconditioned BMMSC-treated animals than in other groups. In addition, the perioperative injection of VEGF neutralizing antibody significantly blocked the therapeutic effects of hypoxia preconditioned BMMSCs on liver injury and regeneration in this model.

Conclusion

Hypoxia preconditioned BMMSCs enhanced liver regeneration after massive hepatectomy in rats, possibly by upregulating the level of VEGF.
Quantification of a Proteotypic Peptide from Protein C Inhibitor by Liquid Chromatography–Free SISCAPA-MALDI Mass Spectrometry: Application to Identification of Recurrence of Prostate Cancer
Authors: Razavi Morteza , Johnson Lisa DS , Lum Julian J , Kruppa Gary , Anderson N Leigh , Pearson Terry W
Published: July 2013

BACKGROUND

Biomarker validation remains one of the most challenging constraints to the development of new diagnostic assays. To facilitate biomarker validation, we previously developed a chromatography-free stable isotope standards and capture by antipeptide antibodies (SISCAPA)-MALDI assay allowing rapid, high-throughput quantification of protein analytes in large sample sets. Here we applied this assay to the measurement of a surrogate proteotypic peptide from protein C inhibitor (PCI) in sera from patients with prostate cancer.

METHODS

A 2-plex SISCAPA-MALDI assay for quantification of proteotypic peptides from PCI and soluble transferrin receptor (sTfR) was used to measure these peptides in 159 trypsin-digested sera collected from 51 patients with prostate cancer. These patients had been treated with radiation with or without neoadjuvant androgen deprivation.

RESULTS

Patients who experienced biochemical recurrence of prostate cancer showed decreased serum concentrations of the PCI peptide analyte within 18 months of treatment. The PCI peptide concentrations remained increased in the sera of patients who did not experience cancer recurrence. Prostate-specific antigen concentrations had no predictive value during the same time period.

CONCLUSIONS

The high-throughput, liquid chromatography–free SISCAPA-MALDI assay is capable of rapid quantification of proteotypic PCI and sTfR peptide analytes in complex serum samples. Decreased serum concentrations of the PCI peptide were found to be related to recurrence of prostate cancer in patients treated with radiation with or without hormone therapy. However, a larger cohort of patients will be required for unequivocal validation of the PCI peptide as a biomarker for clinical use.
Healthy ageing: The Get a Life Marching Band
Authors: Frerich Jessica Bentley
Published: July 2013

This article features the Get a Life Marching Band, an American adult marching band in Portland, Oregon formed in 1994. My study began during Mardi Gras celebrations in New Orleans in 2010 and involved observations, interviews and a survey of members. The primary themes that emerged from my investigation were that band members strive to have fun, spread the joy of music and share their energy with the audience. In this report I discuss the organization’s history, mission, purpose, activities, people and impact.
The Effects of Transactions Costs and Social Distance: Evidence from a Field Experiment
Authors: Meer Jonathan , Rigbi Oren
Published: July 2013

We use data from a field experiment at Kiva, the online microfinance platform, to examine the role of transactions costs and social distance in decision-making. Requests for loans are either written in English or another language, and our treatment consists of posting requests in the latter category with or without translation. We find evidence that relatively small transactions costs have a large effect on the share of funding coming from speakers of languages other than that in which the request was written. Social distance plays a smaller role in funding decisions.
Possibilities of preparation of exotic radionuclide samples at PSI for scientific investigations
Authors: Schumann D. , Stowasser T. , Dressler R. , Ayranov M.
Published: July 2013

The interactions of high-energy protons with matter produce a large variety of radionuclides due to the diversity of the induced nuclear reactions. Some of those isotopes are very rare, exotic, and, in many cases, difficult to produce by complementary methods. Valuable isotopes, interesting for scientific and technological applications, can be extracted from samples stemming from the surroundings or components of a proton accelerator, in particular if the load of the initial particle current is relatively high (esp. in the Megawatt range). Since PSI operates one of the most powerful high-energy proton accelerators world-wide, this facility is best-suited for an R&D program aimed at “harvesting” such isotopes. An initiative called ERAWAST (Exotic Radionuclides from Accelerator Waste for Science and Technology) was started in 2006 in order to identify and motivate potential users. After six years, first achievements as well as realistic future plans for front-end experiments are available. The present contribution describes radiochemical separation techniques for selected examples, summarizes the most prominent results and gives an outlook on the upcoming experiments within the scope of the ERAWAST program.
Synthesis and Characterization of Yttrium Titanate and Er doped Yttrium Titanate Nanofibers
Authors: Mondal Kanchan , Hartman Kaleb , Trifon George , Dasgupta Debalina , Bolin Matthew , Dasari Mallika , Tsai Chung Ying
Published: July 2013

Yttrium titanate belongs to a family of compounds called pyrochlores with significant properties such as ionic conduction, optical non-linearity and radiation tolerance that have resulted in applications thermal barrier coatings, high-permittivity dielectrics, and materials for safe disposal of actinide-containing nuclear waste, and hydrogen storage material. The application of these materials in ODS ferritic steels, photocatalytic water splitting and a more efficient host material than TiO2 for Er3+ luminescence have been evaluated. ErxY2-xTi2O7 has tremendous applications in fiber amplifiers, integrated optical devices and selective emitters for thermophotovoltaic applications. Since 1-D nanostructures are deemed to be important building blocks for future optical and optoelectronic nanodevices, we have used electrospinning methods to synthesize nanofibers and freestanding, non-woven nanofibers membranes of single phase yttrium titanate and ErxY2-xTi2O7 (Er/(Ti+Er) at. ratio= 0 -15 %) with diameters less than 150 nm and have characterized the physical, thermal and optical properties of these nanofibers.