bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of the Structure and Hydraulic Function of Bordered Pits Using the Lattice Boltzman Method
Authors: Qu Wen , Yang Chunmei , Ma Yan , Yu Wenji , Qin Guangyi , Jin Yufei
Published: April 2021

Fluid flow between adjacent tracheids is realized through bordered pits in the xylem of conifers. The pit has an extremely small size and a highly complex structure. This paper presents a mesoscopic analytical method for the relationship between the pit structure and its hydraulic characteristics through mathematical modeling using the lattice Boltzmann method (LBM) and curved boundary treatment. Mongolian Scots pine were selected as the research subject of this study, and the bordered pit structure parameters was collected by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and the original geometric features were maintained for direct modeling analysis. The model revealed the relationship between various components of the bordered pit and liquid flow velocity/resistance, indicating that margo is the main factor affecting flow resistance. Further anatomical investigation separately analyzed the influence of change in a single factor, including pit diameter, pit aperture diameter, pit depth, torus diameter, and margo thickness, on the overall flow and pressure drop to confirm the importance of various factors in this relationship. Additionally, the influence of pore size and pore location distribution in the margo on the flow rate and pressure drop was further analyzed quantitatively. The results showed that the flow rate through individual pores is the result of the combined effect of pore area and radial position of the pore in the margo. Our study promotes the research and application of the mesoscopic model LBM in simulating flow conditions in the complex flow field of pits, which realizes the numerical analysis of the flow field model based on individualized real bordered pits. In comparison with the classical macroscopic model, the accuracy and effectiveness of the proposed model are proved. This research can provide a promising method for analyzing the physiological and ecological functions of conifer and realizing the efficient utilization of wood resources.
A Surrogate Model for Rapidly Assessing the Size of a Wildfire over Time
Authors: KC Ujjwal , Aryal Jagannath , Hilton James , Garg Saurabh
Published: April 2021

Rapid estimates of the risk from potential wildfires are necessary for operational management and mitigation efforts. Computational models can provide risk metrics, but are typically deterministic and may neglect uncertainties inherent in factors driving the fire. Modeling these uncertainties can more accurately predict risks associated with a particular wildfire, but requires a large number of simulations with a corresponding increase in required computational time. Surrogate models provide a means to rapidly estimate the outcome of a particular model based on implicit uncertainties within the model and are very computationally efficient. In this paper, we detail the development of a surrogate model for the growth of a wildfire based on initial meteorological conditions: temperature, relative humidity, and wind speed. Multiple simulated fires under different conditions are used to develop the surrogate model based on the relationship between the area burnt by the fire and each meteorological variable. The results from nine bio-regions in Tasmania show that the surrogate model can closely represent the change in the size of a wildfire over time. The model could be used for a rapid initial estimate of likely fire risk for operational wildfire management.
Investigating Cellular Trajectories in the Severity of COVID-19 and Their Transcriptional Programs Using Machine Learning Approaches
Authors: Jeong Hyun-Hwan , Jia Johnathan , Dai Yulin , Simon Lukas M. , Zhao Zhongming
Published: April 2021

Single-cell RNA sequencing of the bronchoalveolar lavage fluid (BALF) samples from COVID-19 patients has enabled us to examine gene expression changes of human tissue in response to the SARS-CoV-2 virus infection. However, the underlying mechanisms of COVID-19 pathogenesis at single-cell resolution, its transcriptional drivers, and dynamics require further investigation. In this study, we applied machine learning algorithms to infer the trajectories of cellular changes and identify their transcriptional programs. Our study generated cellular trajectories that show the COVID-19 pathogenesis of healthy-to-moderate and healthy-to-severe on macrophages and T cells, and we observed more diverse trajectories in macrophages compared to T cells. Furthermore, our deep-learning algorithm DrivAER identified several pathways (e.g., xenobiotic pathway and complement pathway) and transcription factors (e.g., MITF and GATA3) that could be potential drivers of the transcriptomic changes for COVID-19 pathogenesis and the markers of the COVID-19 severity. Moreover, macrophages-related functions corresponded more to the disease severity compared to T cells-related functions. Our findings more proficiently dissected the transcriptomic changes leading to the severity of a COVID-19 infection.
Fault-Based Geological Lineaments Extraction Using Remote Sensing and GIS—A Review
Authors: Ahmadi Hemayatullah , Pekkan Emrah
Published: April 2021

Geological lineaments are the earth’s linear features indicating significant tectonic units in the crust associated with the formation of minerals, active faults, groundwater controls, earthquakes, and geomorphology. This study aims to provide a systematic review of the state-of-the-art remote sensing techniques and data sets employed for geological lineament analysis. The critical challenges of this approach and the diverse data verification and validation techniques will be presented. Thus, this review spanned academic articles published since 1975, including expert reports and theses. Landsat series, Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER), Sentinel 2 are the prevalent optical remote sensing data widely used for lineament detection. Moreover, Shuttle Radar Topography Mission (SRTM) derived Digital Elevation Model (DEM), Synthetic-aperture radar (SAR), Interferometric synthetic aperture radar (InSAR), and Sentinel 1 are the typical radar remotely sensed data which are widely used for the detection of geological lineaments. The geological lineaments acquired via GIS techniques are not consistent even though a variety of manual, semi-automated, and automated techniques are applied. Therefore, a single method may not provide an accurate lineament distribution and may include artifacts requiring integration of multiple algorithms, e.g., manual and automated algorithms.
Mediating Roles of Cognitive Complaints on Relationships between Insomnia, State Anxiety, and Presenteeism in Japanese Adult Workers
Authors: Toyoshima Kuniyoshi , Inoue Takeshi , Shimura Akiyoshi , Uchida Yoshihiro , Masuya Jiro , Fujimura Yota , Higashi Shinji , Kusumi Ichiro
Published: April 2021

Complaints of cognitive functions (CCFs), defined as subjective cognitive dysfunction, affect social function; additionally, for workers, this condition is an important factor in presenteeism and mediates the effect of depressive symptoms on presenteeism. This study aimed to investigate whether CCFs mediate the relationships among insomnia, state anxiety (SA), and presenteeism. Participants were 471 Japanese adult workers evaluated using the Athens Insomnia Scale, State-Trait Anxiety Inventory (Form Y), Cognitive Complaints in Bipolar Disorder Rating Assessment, and Work Limitations Questionnaire 8 to assess insomnia, SA, CCFs, and presenteeism, respectively. Path analysis was used to evaluate the correlations between variables. CCFs significantly mediated the associations among insomnia, SA, and presenteeism. To address the presenteeism associated with insomnia and SA, it may be useful to assess the mediating roles of CCFs.
Health Literacy Co-Design in a Low Resource Setting: Harnessing Local Wisdom to Inform Interventions across Fishing Villages in Egypt to Improve Health and Equity
Authors: Anwar Wagida A. , Mostafa Nayera S. , Hakim Sally Adel , Sos Dalia G. , Cheng Christina , Osborne Richard H.
Published: April 2021

Fishermen in low resource settings have limited access to health services and may have a range of health literacy-related difficulties that may lead to poor health outcomes. To provide solutions and interventions based on their needs, co-design is considered best practice in such settings. This study aimed to implement a co-design process as a step towards developing health literacy interventions to improve health and equity in the Borollos Lake region of northern Egypt, a low resource setting with a high prevalence of chronic diseases. This study was guided by the Ophelia (Optimising Health Literacy and Access) process, a widely used and flexible co-design process that seeks to create local and fit-for-purpose health literacy solutions through genuine engagement and participation of community members and relevant stakeholders. Following a health literacy survey using the Health Literacy Questionnaire (HLQ), cluster analysis was conducted to identify the diverse health literacy profiles among the fishing communities. Seven health literacy profiles were identified. Vignettes, representing these profiles, were presented and discussed in ideas generation/co-design workshops with fishermen and health workers to develop intervention ideas. Seventeen fishermen, 22 wives of fishermen, and 20 nurses participated in four workshops. Fifteen key strategies across five themes, including ‘Enhancing education among fishing communities’, ‘Provide good quality health services’, ‘Financial support for health’, ‘Social support for health’, and ‘Promote better health-related quality of life among fishermen’, were generated. The ideas did not only target the individuals but also required actions from the government, non-government organizations, and fishermen syndicates. By harnessing local wisdom, the Ophelia process has created meaningful engagement with the local communities, leading to a wide range of practical and feasible solutions that match the special needs and environment of a low resource setting.
Assessing the Impact of a Hilly Environment on Depressive Symptoms among Community-Dwelling Older Adults in Japan: A Cross-Sectional Study
Authors: Abe Takafumi , Okuyama Kenta , Hamano Tsuyoshi , Takeda Miwako , Yamasaki Masayuki , Isomura Minoru , Nakano Kunihiko , Sundquist Kristina , Nabika Toru
Published: April 2021

Although some neighborhood environmental factors have been found to affect depressive symptoms, few studies have focused on the impact of living in a hilly environment, i.e., land slope, on depressive symptoms among rural older adults. This cross-sectional study aimed to investigate whether a land slope is associated with depressive symptoms among older adults living in rural areas. Data were collected from 935 participants, aged 65 years and older, who lived in Shimane prefecture, Japan. Depressive symptoms were assessed using the Zung Self-Rating Depression Scale (SDS) and defined on the basis of an SDS score ≥ 40. Land slopes within a 400 m network buffer were assessed using geographic information systems. Odds ratios (ORs) with 95% confidence intervals (CIs) of depressive symptoms were estimated using logistic regression. A total of 215 (23.0%) participants reported depressive symptoms. The land slope was positively associated with depressive symptoms (OR = 1.04; 95% CI = 1.01–1.08) after adjusting for all confounders. In a rural setting, living in a hillier environment was associated with depressive symptoms among community-dwelling older adults in Japan.
The Interplay of Socioecological Determinants of Work–Life Balance, Subjective Wellbeing and Employee Wellbeing
Authors: Wong Ka Po , Lee Fion Choi Hung , Teh Pei-Lee , Chan Alan Hoi Shou
Published: April 2021

Today’s workers are struggling to achieve a balance between their work and personal life roles because of both specific needs and limited resources. This study explored the socioecological factors that influence work–life balance (WLB) and how they operate. The relationships between WLB, subjective wellbeing, employee wellbeing and quality time allocation were examined. A total of 1063 responses were received, using an online survey. The results show that relational, community and societal factors directly influenced the individual factors and were indirectly associated with perceived WLB. Individual factors (i.e., personal feelings, behaviours and health) were found to be the crucial determinants of an individual’s perceived WLB. It was found that WLB positively correlated with employee wellbeing and quality and quantity of personal life-time. Subjective wellbeing was found to be a significant moderator in the relationship between WLB and its outcomes. This study demonstrated the process of how workers determine their own WLB by applying the socioecological framework for categorising the determinants and suggested new avenues that improve the whole wellbeing of workers and also foster long-term development of organisations.
A Case of Silicone and Sarcoid Granulomas in a Patient with “Highly Cohesive” Silicone Breast Implants: A Histopathologic and Laser Raman Microprobe Analysis
Authors: Todorov Todor I. , de Bakker Erik , Smith Diane , Langenberg Lisette C. , Murakata Linda A. , Kramer Mark H. H. , Centeno Jose A. , Nanayakkara Prabath W. B.
Published: April 2021

Foreign body giant cell (FBGC) reaction to silicone material in the lymph nodes of patients with silicone breast implants has been documented in the literature, with a number of case reports dating back to 1978. Many of these case reports describe histologic features of silicone lymphadenopathy in regional lymph nodes from patients with multiple sets of different types of implants, including single lumen smooth surface gel, single lumen textured surface gel, single lumen with polyethylene terephthalate patch, single lumen with polyurethane coating, and double lumen smooth surface. Only one other case report described a patient with highly-cohesive breast implants and silicone granulomas of the skin. In this article, we describe a patient with a clinical presentation of systemic sarcoidosis following highly cohesive breast implant placement. Histopathologic analysis and Confocal Laser Raman Microprobe (CLRM) examination were used to confirm the presence of silicone in the axillary lymph node and capsular tissues. This is the first report where chemical spectroscopic mapping has been used to establish and identify the coexistence of Schaumann bodies, consisting of calcium oxalate and calcium phosphate minerals, together with silicone implant material.
How Much Money Do You Need to Feel Taller? Impact of Money on Perception of Body Height
Authors: Walczak Radosław , Zdybek Przemysław , Giuliani Felice , Tommasi Luca
Published: April 2021

Body height is considered to be one of the most important reproductive signals. However, there are only a few publications on what influences the sense of whether we assess ourselves as tall or short. In the present contribution, the psychological impact of money on the evaluation of a person’s own height was tested. We performed two experimental studies in which the respondents had contact with different amounts of money and were asked to evaluate their body height with the use of a laser pointer. The first experiment (N = 61) showed that contact with money significantly increased subjective height evaluation, and the effect was independent of participants’ real body height. The second experiment (N = 120) replicated the effect of money manipulation. Moreover, it was shown that higher amounts of money increased one’s own height estimation more than smaller amounts. Our research shows that money can be used for building one’s social position, which is an attractiveness signal that can influence one’s own height evaluation.
SGLT2is and Renal Protection: From Biological Mechanisms to Real-World Clinical Benefits
Authors: Leoncini Giovanna , Russo Elisa , Bussalino Elisabetta , Barnini Cecilia , Viazzi Francesca , Pontremoli Roberto
Published: April 2021

In recent years, following the publication of results from several RCTs, first on cardiovascular and more recently on renal outcomes, SGLT2is have become the standard of care to prevent diabetic kidney disease and slow its progression. This narrative review focuses on biological mechanisms, both renal and extrarenal, underlying kidney protection with SGLT2is. Furthermore, data from cardiovascular as well as renal outcome trials, mostly conducted in diabetic patients, are presented and discussed to provide an overview of current uses as well as the future therapeutic potential of these drugs.
Exploring the Biomaterial-Induced Secretome: Physical Bone Substitute Characteristics Influence the Cytokine Expression of Macrophages
Authors: Barbeck Mike , Schröder Marie-Luise , Alkildani Said , Jung Ole , Unger Ronald E.
Published: April 2021

In addition to their chemical composition various physical properties of synthetic bone substitute materials have been shown to influence their regenerative potential and to influence the expression of cytokines produced by monocytes, the key cell-type responsible for tissue reaction to biomaterials in vivo. In the present study both the regenerative potential and the inflammatory response to five bone substitute materials all based on β-tricalcium phosphate (β-TCP), but which differed in their physical characteristics (i.e., granule size, granule shape and porosity) were analyzed for their effects on monocyte cytokine expression. To determine the effects of the physical characteristics of the different materials, the proliferation of primary human osteoblasts growing on the materials was analyzed. To determine the immunogenic effects of the different materials on human peripheral blood monocytes, cells cultured on the materials were evaluated for the expression of 14 pro- and anti-inflammatory cytokines, i.e., IL-6, IL-10, IL-1β, VEGF, RANTES, IL-12p40, I-CAM, IL-4, V-CAM, TNF-α, GM-CSF, MIP-1α, Il-8 and MCP-1 using a Bio-Plex® Multiplex System. The granular shape of bone substitutes showed a significant influence on the osteoblast proliferation. Moreover, smaller pore sizes, round granular shape and larger granule size increased the expression of GM-CSF, RANTES, IL-10 and IL-12 by monocytes, while polygonal shape and the larger pore sizes increased the expression of V-CAM. The physical characteristics of a bone biomaterial can influence the proliferation rate of osteoblasts and has an influence on the cytokine gene expression of monocytes in vitro. These results indicate that the physical structure of a biomaterial has a significant effect of how cells interact with the material. Thus, specific characteristics of a material may strongly affect the regenerative potential in vivo.
Application of Supervised SOM Algorithms in Predicting the Hepatotoxic Potential of Drugs
Authors: Drgan Viktor , Bajželj Benjamin
Published: April 2021

The hepatotoxic potential of drugs is one of the main reasons why a number of drugs never reach the market or have to be withdrawn from the market. Therefore, the evaluation of the hepatotoxic potential of drugs is an important part of the drug development process. The aim of this work was to evaluate the relative abilities of different supervised self-organizing algorithms in classifying the hepatotoxic potential of drugs. Two modifications of standard counter-propagation training algorithms were proposed to achieve good separation of clusters on the self-organizing map. A series of optimizations were performed using genetic algorithm to select models developed with counter-propagation neural networks, X-Y fused networks, and the two newly proposed algorithms. The cluster separations achieved by the different algorithms were evaluated using a simple measure presented in this paper. Both proposed algorithms showed a better formation of clusters compared to the standard counter-propagation algorithm. The X-Y fused neural network confirmed its high ability to form well-separated clusters. Nevertheless, one of the proposed algorithms came close to its clustering results, which also resulted in a similar number of selected models.
Transcriptome Analysis of Seed Weight Plasticity in Brassica napus
Authors: Canales Javier , Verdejo José , Carrasco-Puga Gabriela , Castillo Francisca M. , Arenas-M Anita , Calderini Daniel F.
Published: April 2021

A critical barrier to improving crop yield is the trade-off between seed weight (SW) and seed number (SN), which has been commonly reported in several crops, including Brassica napus. Despite the agronomic relevance of this issue, the molecular factors involved in the interaction between SW and SN are largely unknown in crops. In this work, we performed a detailed transcriptomic analysis of 48 seed samples obtained from two rapeseed spring genotypes subjected to different source–sink (S–S) ratios in order to examine the relationship between SW and SN under different field conditions. A multifactorial analysis of the RNA-seq data was used to identify a group of 1014 genes exclusively regulated by the S–S ratio. We found that a reduction in the S–S ratio during seed filling induces the expression of genes involved in sucrose transport, seed weight, and stress responses. Moreover, we identified five co-expression modules that are positively correlated with SW and negatively correlated with SN. Interestingly, one of these modules was significantly enriched in transcription factors (TFs). Furthermore, our network analysis predicted several NAC TFs as major hubs underlying SW and SN compensation. Taken together, our study provides novel insights into the molecular factors associated with the SW–SN relationship in rapeseed and identifies TFs as potential targets when improving crop yield.
Prolactin-Releasing Peptide Differentially Regulates Gene Transcriptomic Profiles in Mouse Bone Marrow-Derived Macrophages
Authors: Sun Yulong , Zuo Zhuo , Kuang Yuanyuan
Published: April 2021

Prolactin-releasing Peptide (PrRP) is a neuropeptide whose receptor is GPR10. Recently, the regulatory role of PrRP in the neuroendocrine field has attracted increasing attention. However, the influence of PrRP on macrophages, the critical housekeeper in the neuroendocrine field, has not yet been fully elucidated. Here, we investigated the effect of PrRP on the transcriptome of mouse bone marrow-derived macrophages (BMDMs) with RNA sequencing, bioinformatics, and molecular simulation. BMDMs were exposed to PrRP (18 h) and were subjected to RNA sequencing. Differentially expressed genes (DEGs) were acquired, followed by GO, KEGG, and PPI analysis. Eight qPCR-validated DEGs were chosen as hub genes. Next, the three-dimensional structures of the proteins encoded by these hub genes were modeled by Rosetta and Modeller, followed by molecular dynamics simulation by the Gromacs program. Finally, the binding modes between PrRP and hub proteins were investigated with the Rosetta program. PrRP showed no noticeable effect on the morphology of macrophages. A total of 410 DEGs were acquired, and PrRP regulated multiple BMDM-mediated functional pathways. Besides, the possible docking modes between PrRP and hub proteins were investigated. Moreover, GPR10 was expressed on the cell membrane of BMDMs, which increased after PrRP exposure. Collectively, PrRP significantly changed the transcriptome profile of BMDMs, implying that PrRP may be involved in various physiological activities mastered by macrophages.
AS-Quant: Detection and Visualization of Alternative Splicing Events with RNA-seq Data
Authors: Fahmi Naima Ahmed , Nassereddeen Heba , Chang Jaewoong , Park Meeyeon , Yeh Hsinsung , Sun Jiao , Fan Deliang , Yong Jeongsik , Zhang Wei
Published: April 2021

(1) Background: A simplistic understanding of the central dogma falls short in correlating the number of genes in the genome to the number of proteins in the proteome. Post-transcriptional alternative splicing contributes to the complexity of the proteome and is critical in understanding gene expression. mRNA-sequencing (RNA-seq) has been widely used to study the transcriptome and provides opportunity to detect alternative splicing events among different biological conditions. Despite the popularity of studying transcriptome variants with RNA-seq, few efficient and user-friendly bioinformatics tools have been developed for the genome-wide detection and visualization of alternative splicing events. (2) Results: We propose AS-Quant, (Alternative Splicing Quantitation), a robust program to identify alternative splicing events from RNA-seq data. We then extended AS-Quant to visualize the splicing events with short-read coverage plots along with complete gene annotation. The tool works in three major steps: (i) calculate the read coverage of the potential spliced exons and the corresponding gene; (ii) categorize the events into five different categories according to the annotation, and assess the significance of the events between two biological conditions; (iii) generate the short reads coverage plot for user specified splicing events. Our extensive experiments on simulated and real datasets demonstrate that AS-Quant outperforms the other three widely used baselines, SUPPA2, rMATS, and diffSplice for detecting alternative splicing events. Moreover, the significant alternative splicing events identified by AS-Quant between two biological contexts were validated by RT-PCR experiment. (3) Availability: AS-Quant is implemented in Python 3.0. Source code and a comprehensive user’s manual are freely available online.
Improvement of the Diagnostic Performance of Facial Neuritis Using Contrast-Enhanced 3D T1 Black-Blood Imaging: Comparison with Contrast-Enhanced 3D T1-Spoiled Gradient-Echo Imaging
Authors: Lee Seun-Ah , Jo Sang-Won , Chang Suk-Ki , Kwon Ki-Han
Published: April 2021

This study aims to investigate the diagnostic ability of the contrast-enhanced 3D T1 black-blood fast spin-echo (T1 BB-FSE) sequence compared with the contrast-enhanced 3D T1-spoiled gradient-echo (CE-GRE) sequence in patients with facial neuritis. Forty-five patients with facial neuritis who underwent temporal bone MR imaging, including T1 BB-FSE and CE-GRE imaging, were examined. Two reviewers independently assessed the T1 BB-FSE and CE-GRE images in terms of diagnostic performance, and qualitative (diagnostic confidence and visual asymmetric enhancement) and quantitative analysis (contrast-enhancing lesion extent of the canalicular segment of the affected facial nerve (LEC) and the affected side-to-normal signal intensity ratio (rSI)). The AUCs of each reviewer, and the sensitivity and accuracy of T1 BB-FSE were significantly superior to those of CE-GRE (p < 0.05). Regarding diagnostic confidence and visual asymmetric enhancement, T1 BB-FSE tended to be rated greater than CE-GRE (p < 0.05). Additionally, in quantitative analysis, LEC and rSI of the canalicular segment on T1 BB-FSE were larger than those on CE-GRE (p < 0.05). The T1 BB-FSE sequence was significantly superior to the CE-GRE sequence, with more conspicuous lesion visualization in terms of both qualitative and quantitative aspects in patients with facial neuritis.
Patient-Reported Outcomes, Health-Related Quality of Life, and Clinical Outcomes for Urothelial Cancer Patients Receiving Chemo- or Immunotherapy: A Real-Life Experience
Authors: Taarnhøj Gry Assam , Lindberg Henriette , Johansen Christoffer , Pappot Helle
Published: April 2021

Patients with urothelial cell carcinoma (UCC) often have comorbidities, which cause trouble for the completion of oncological treatment, and little is known about their quality of life (QoL). The aim of the present study was to obtain and describe patient-reported outcomes (PRO) and QoL data from UCC patients in the treatment for locally advanced muscle-invasive or metastatic UCC. A total of 79 patients with UCC completed four questionnaires (EORTC QLQ-C30, QLQ-BLM30, HADS, and select PRO-CTCAE™ questions) once weekly during their treatment. From those, 26 patients (33%) underwent neoadjuvant treatment for local disease while 53 patients (67%) were treated for metastatic disease. Of all patients, 54% did not complete the planned treatment due to progression, nephrotoxicity, death, or intolerable symptoms during treatment. The five most prevalent PRO-CTCAE grade ≥ 2 symptoms were frequent urination (37%), fatigue (35%), pain (31%), dry mouth (23%), and swelling of the arms or legs (23%). The baseline mean overall QoL was 61 (±SD 24) for all patients (neoadjuvant (73, ±SD 19) and metastatic (54, ±SD 24)) and remained stable over the course of treatment for both groups. A stable overall QoL was observed for the patients in this study. More than half of the patients did not, however, complete the planned treatment. Further supportive care is warranted for bladder cancer patients.
Glomerular Diseases in Diabetic Patients: Implications for Diagnosis and Management
Authors: Oliva-Damaso Nestor , Mora-Gutiérrez José María , Bomback Andrew S.
Published: April 2021

The prevalence of diabetes continues to rise worldwide. In addition to rising rates of diabetic kidney disease, we are also seeing a parallel rise in nondiabetic kidney disease among patients with diabetes. These nondiabetic lesions include focal segmental glomerulosclerosis, IgA nephropathy, membranous nephropathy, and other glomerular diseases. The management of diabetic kidney disease is rapidly evolving to include, beyond glycemic control and renin angiotensin inhibition, the use of sodium-glucose cotransporter 2 (SGLT2) inhibitors and mineralocorticoid receptor antagonists. These and other new treatment strategies should be applicable to managing glomerular disease in diabetic patients to reduce toxicities associated with immunosuppression and, in particular, corticosteroids. The prevalence of glomerular disease in diabetic patients is underappreciated. Diagnosis and appropriately treating these diseases remain an important avenue to modify kidney outcomes in diabetic patients.
Inhibition of Cyclooxygenase-2 Alters Craniofacial Patterning during Early Embryonic Development of Chick
Authors: Parmar Bhaval , Verma Urja , Khaire Kashmira , Danes Dhanush , Balakrishnan Suresh
Published: April 2021

A recent study from our lab revealed that the inhibition of cyclooxygenase-2 (COX-2) exclusively reduces the level of PGE2 (Prostaglandin E2) among prostanoids and hampers the normal development of several structures, strikingly the cranial vault, in chick embryos. In order to unearth the mechanism behind the deviant development of cranial features, the expression pattern of various factors that are known to influence cranial neural crest cell (CNCC) migration was checked in chick embryos after inhibiting COX-2 activity using etoricoxib. The compromised level of cell adhesion molecules and their upstream regulators, namely CDH1 (E-cadherin), CDH2 (N-cadherin), MSX1 (Msh homeobox 1), and TGF-β (Transforming growth factor beta), observed in the etoricoxib-treated embryos indicate that COX-2, through its downstream effector PGE2, regulates the expression of these factors perhaps to aid the migration of CNCCs. The histological features and levels of FoxD3 (Forkhead box D3), as well as PCNA (Proliferating cell nuclear antigen), further consolidate the role of COX-2 in the migration and survival of CNCCs in developing embryos. The results of the current study indicate that COX-2 plays a pivotal role in orchestrating craniofacial structures perhaps by modulating CNCC proliferation and migration during the embryonic development of chicks.
Emerging Invasive Fungal Infections in Critically Ill Patients: Incidence, Outcomes and Prognosis Factors, a Case-Control Study
Authors: Larcher Romaric , Platon Laura , Amalric Matthieu , Brunot Vincent , Besnard Noemie , Benomar Racim , Daubin Delphine , Ceballos Patrice , Rispail Philippe , Lachaud Laurence , Bourgeois Nathalie , Klouche Kada
Published: April 2021

Comprehensive data on emerging invasive fungal infections (EIFIs) in the critically ill are scarce. We conducted a case-control study to characterize EIFIs in patients admitted to a French medical ICU teaching hospital from 2006 to 2019. Among 6900 patients, 26 (4 per 1000) had an EIFI: Mucorales accounted for half, and other isolates were mainly Saprochaete, Fusarium and Scedosporium. EIFIs occurred mostly in patients with immunosuppression and severe critical illness. Antifungal treatments (mainly amphotericin B) were administered to almost all patients, whereas only 19% had surgery. In-ICU, mortality was high (77%) and associated with previous conditions such as hematological malignancy or cancer, malnutrition, chronic kidney disease and occurrence of acute respiratory distress syndrome and/or hepatic dysfunction. Day-90 survival rates, calculated by the Kaplan–Meier method, were similar between patients with EIFIs and a control group of patients with aspergillosis: 20%, 95% CI (9- 45) versus 18%, 95% CI (8- 45) (log-rank: p > 0.99). ICU management of such patients should be assessed on the basis of underlying conditions, reversibility and acute event severity rather than the mold species.
In Vitro Antibacterial, Antifungal, Nematocidal and Growth Promoting Activities of Trichoderma hamatum FB10 and Its Secondary Metabolites
Authors: Baazeem Alaa , Almanea Abdulaziz , Manikandan Palanisamy , Alorabi Mohammed , Vijayaraghavan Ponnuswamy , Abdel-Hadi Ahmed
Published: April 2021

Microbial natural biocides have attracted much more attention in recent years in order to avoid the unrestricted use of chemical biocides in the environment. The aim of this study is to analyze the antibacterial and antifungal activities of secondary metabolites and growth promoting, nematicidal, and soil enzyme activity mediated by Trichoderma hamatum FB10. The bactericidal and fungicidal activities were performed using cell-free extract. Results revealed that the selected strain exert antibacterial activity against Acidovorax avenae, Erutimacarafavora, and Xanthomonas campestris. The selected fungal strain FB10 showed antagonistic activity against fungal pathogens such as, S. sclerotiorum, Rhizoctonia solani, Alternaria radicina, Alternaria citri, and Alternaria dauci. Among the bacterial pathogens, A. avenae showed least MIC (30 ± 2.5 µg/mL) and MBC (70 ± 1.25 µg/mL) values. T. hamatum FB10 strain synthesized bioactive volatile secondary metabolite, which effectively inhibited the growth of bacteria and fungi and indicated the presence of 6-pentyl-alpha-pyrone as the major compound (67.05%). The secondary metabolite synthesized by T. hamatum FB10 showed nematicidal activity against M. incognita eggs. Egg hatch inhibition was 78 ± 2.6% and juvenile stage mortality rate was 89 ± 2.5% when the strain FB10 was treated with nematode. The cell free extract of T. hamatum FB10 showed protease, amylase, cellulase, chitinase, glucanase activities. T. hamatum FB10 inoculated with green gram increased 11% plant height, compared to the control. The fresh weight of the experimental group inoculated with T. hamatum FB10 increased 33.6% more compared to the control group. The green gram seedlings inoculated with T. hamatum FB10 increased 18% more dry weight than control group. Soil enzymes such as, urease, phosphatase, catalase and saccharase were improved in the soil inoculated with T. hamatum FB10. These biochemical components play potent role in soil fertility, energy conversion, and in soil organic matter conversion.
Factors Associated with Postpartum Post-Traumatic Stress Disorder (PTSD) Following Obstetric Violence: A Cross-Sectional Study
Authors: Martinez-Vázquez Sergio , Rodríguez-Almagro Julián , Hernández-Martínez Antonio , Martínez-Galiano Juan Miguel
Published: April 2021

To determine the association between experiencing obstetric violence and the incidence of postpartum post-traumatic stress disorder (PTSD). A cross-sectional study with puerperal women was conducted in Spain following ethical approval. The Perinatal Posttraumatic Stress Disorder Questionnaire (PPQ) was administered online. Sociodemographic, clinical, and obstetric violence variables and the risk of dichotomized PTSD (low/high) were studied by bivariate and multivariate analysis with binary logistic regression. 955 women were invited to participate. 53 women refused to participate, three did not complete all survey questions and, finally, 899 women were included. The risk of PTSD (score ≥ 19) using the PPQ was 12.7% (114). The mean score was 9.10 points (SD = 8.52). Risk factors identified were having a delivery plan that was not respected (aOR: 2.85, 95% CI 1.56–5.21), elective caesarean delivery (aOR: 2.53, 95% CI 1.02–2.26), emergency caesarean section (aOR: 3.58, 95% CI 1.83–6.99), admission of the newborn to the neonatal intermediate care unit (aOR: 4.95, 95% CI 2.36–10.36), admission to the intensive care unit (aOR: 2.25, 95% CI 1.02–4.97), formula feeding on discharge (aOR: 3.57, 95% CI 1.32–9.62), verbal obstetric violence (aOR: 5.07, 95% CI 2.98–8.63), and psycho-affective obstetric violence (aOR: 2.61, 95% CI 1.45–4.67). Various clinical practices were identified with the risk of PTSD, highlighting various types of obstetric violence. Partner support and early breastfeeding were identified as protective factors. Sensitizing professionals is essential to prevent the risk of PTSD.
Hexosylceramides and Glycerophosphatidylcholine GPC(36:1) Increase in Multi-Organ Dysfunction Syndrome Patients with Pediatric Intensive Care Unit Admission over 8-Day Hospitalization
Authors: Leimanis-Laurens Mara , Wolfrum Emily , Ferguson Karen , Grunwell Jocelyn , Sanfilippo Dominic , Prokop Jeremy , Lydic Todd , Rajasekaran Surender
Published: April 2021

Glycero- and sphingo-lipids are important in plasma membrane structure, caloric storage and signaling. An un-targeted lipidomics approach for a cohort of critically ill pediatric intensive care unit (PICU) patients undergoing multi-organ dysfunction syndrome (MODS) was compared to sedation controls. After IRB approval, patients meeting the criteria for MODS were screened, consented (n = 24), and blood samples were collected from the PICU at HDVCH, Michigan; eight patients needed veno-arterial extracorporeal membrane oxygenation (VA ECMO). Sedation controls were presenting for routine sedation (n = 4). Plasma lipid profiles were determined by nano-electrospray (nESI) direct infusion high resolution/accurate mass spectrometry (MS) and tandem mass spectrometry (MS/MS). Biostatistics analysis was performed using R v 3.6.0. Sixty-one patient samples over three time points revealed a ceramide metabolite, hexosylceramide (Hex-Cer) was high across all time points (mean 1.63–3.19%; vs. controls 0.22%). Fourteen species statistically differentiated from sedation controls (p-value ≤ 0.05); sphingomyelin (SM) [SM(d18:1/23:0), SM(d18:1/22:0), SM(d18:1/23:1), SM(d18:1/21:0), SM(d18:1/24:0)]; and glycerophosphotidylcholine (GPC) [GPC(36:01), GPC(18:00), GPC(O:34:02), GPC(18:02), GPC(38:05), GPC(O:34:03), GPC(16:00), GPC(40:05), GPC(O:36:03)]. Hex-Cer has been shown to be involved in viral infection and may be at play during acute illness. GPC(36:01) was elevated in all MODS patients at all time points and is associated with inflammation and brain injury.
Adherence to Anti-Osteoporotic Treatment and Clinical Implications after Hip Fracture: A Systematic Review
Authors: Dobre Ramona , Niculescu Dan Alexandru , Petca Răzvan-Cosmin , Popescu Răzvan-Ionuț , Petca Aida , Poiană Cătălina
Published: April 2021

The role of anti-osteoporotic treatment as part of the secondary prevention after hip fracture in terms of mortality and re-fracture risk has been studied, and the results are promising. Decreased treatment adherence and compliance is a problem that needs to be addressed by healthcare professionals. A systematic review of the literature was performed using the PubMed database with terms that included hip fracture, mortality, second fracture, and specific anti-osteoporotic treatment. We included 28 articles, 21 regarding mortality and 20 re-fracture rates in hip fracture patients. All studies showed lower mortality after hip fracture associated with anti-osteoporotic treatment, mostly bisphosphonate agents. The re-fracture risk is still debatable, since conflicting data were found. Although most of the studies showed notable effects on mortality and re-fracture rates associated with anti-osteoporotic treatment, we still need more data to validate the actual results.
Clinical Remission Using Personalized Low-Dose Intravenous Infusions of N-acetylcysteine with Minimal Toxicities for Interstitial Cystitis/Bladder Pain Syndrome
Authors: Maharaj Dipnarine , Srinivasan Gayathri , Makepeace Sarah , Hickey Christopher J. , Gouvea Jacqueline
Published: April 2021

Interstitial Cystitis or Bladder Pain Syndrome (IC/BPS) is a heterogeneous condition characterized by elevated levels of inflammatory cytokines, IL-1β, IL-6, IL-8, IL-10, TNF-α, and is associated with debilitating symptoms of pelvic pain and frequent urination. A standard of care for IC/BPS has not been established, and most patients must undergo a series of different treatment options, with potential for severe adverse events. Here, we report a patient with a 26-year history of IC/BPS following treatment with multiple therapies, including low doses of etodolac, amitriptyline and gabapentin, which she was unable to tolerate because of adverse effects, including headaches, blurred vision and cognitive impairment. The patient achieved a complete clinical remission with minimal adverse events after 16 cycles of N-acetylcysteine (NAC) intravenous (IV) infusions over a period of 5 months, and pro-inflammatory cytokine levels were reduced when compared to measurements taken at presentation. Personalized low dose NAC IV infusion therapy represents an effective, safe, anti-inflammatory therapy administered in the outpatient setting for IC/BPS, and warrants further investigation.
Land-Use Modes of the Dike–Pond System in the Pearl River Delta of China and Implications for Rural Revitalization
Authors: Li Haicong , Wang Lu , Gong Jianzhou , Zhu A-Xing , Hu Yueming
Published: April 2021

Rural revitalization is a global problem. The measures should be adjusted to local conditions to make targeted efforts. Natural and socioeconomic resource factors should be considered in rural revitalization. Therefore, this study focuses on the dike–pond system, which is an important traditional agricultural cultural heritage in the Pearl River Delta of China, to illustrate the importance of identifying the utilization mode of a certain land-use type in village integrated with socioeconomic factors to promote rural revitalization. The study used principal component analysis (PCA) and the variance inflation factor (VIF) to identify the main factors influencing the land-use modes of the dike–pond systems, systematic cluster analysis to identify the modes, and interpretive structural modeling to clarify the influence relationships and structures of the factors. We found that the seven modes reflected the different characteristics, organizational structures, and interaction relationships of the factors. There were significant differences in the ecological processes between the seven modes. More detailed village planning should be performed. Strengthening the economic affordability of the operator should be regarded as important in policy guidance and support measures. Agricultural support measures need to be adjusted to different land-use type systems, and localized resources should be revitalized by the theory of “human–earth–sphere”.
The Scenic Beauty of Geosites and Its Relation to Their Scientific Value and Geoscience Knowledge of Tourists: A Case Study from Southeastern Spain
Authors: Tessema Getaneh Addis , Poesen Jean , Verstraeten Gert , Van Rompaey Anton , van der Borg Jan
Published: April 2021

Scenic beauty is one of the most-commonly used indicators in the inventory and assessment of geosites for geoconservation, geoheritage management and geotourism development. It is an important driver of tourists to visit natural areas and it also provides support for the protection of natural heritage. Previous studies on scenic beauty mainly focused on landscape preference and physical characteristics of geosites that affect scenic beauty appreciation. The relationships between the scenic beauty of geosites, their scientific value and the geoscience knowledge of tourists has not been empirically investigated in detail. Hence, this study investigates this relationship using 34 geosites from southeastern Spain. For this purpose, 29 respondents with a geoscience background and who all visited the 34 geosites, 43 respondents with a geoscience background but who did not visit the geosites, and 104 respondents with no geoscience background and who did not visit the geosites, participated in a survey. The first group rated the scenic beauty and the scientific value of the geosites based on a direct field visit during which the scientific background of these geosites was given. On the other hand, the latter two groups rated scenic beauty using representative photos of the geosites. A five-point Likert scale was used to rate the scenic beauty and the scientific value of the geosites. We found a significant relationship between the scenic beauty of geosites and their scientific value, and this relationship becomes more significant if the geoscientific knowledge of the respondents increases. One-way ANOVA results indicated that a geoscience background contributed to higher perceived scenic beauty, especially for those geosites that in general were considered as more scenic by all the respondent groups. It was also found that geosites with viewpoints received in general higher scenic beauty and scientific value ratings.
Multilingual Repertoires at Play: Structure and Function in Reported Speech Utterances of Alyawarr Children
Authors: Dixon Sally
Published: April 2021

While there is increasing international interest in approaching language analysis with the prism of repertoire, research on repertoire on the Australian continent is still very much in the shadow of “traditional” language-centric documentary work. This paper will explore the question of how users of Australian, English-lexified contact varieties exploit their multilingual repertoires to achieve local, conversation–organizational ends. Drawing upon a corpus of video recordings from Ipmangker, a Central Australian Aboriginal community, and using the analytical methods of interactional and comparative variationist linguistics, I examine the production of reported speech by four 6- to 7-year-old Alyawarr children in a play session at home. A set of prosodic, phonological, morphological and discourse-pragmatic features are shown to form a coherent set of linguistic elements with which these multilingual children can contrast reported speech from the surrounding talk. Moreover, the use of reported speech in play not only allows the children to organize their interaction, but responds to and constructs the epistemic landscape of play.