bluuebunny/crossref_metadata_2025 huggingface dataset:
[Retracted] Clinical Promotion of Comfort Nursing Combined with Comprehensive Nursing in the Treatment of Severe Stroke Patients with Diabetes in ICU
Authors: Zhang Haiqin , Chu Hongmei , Qian Xiaoli , Zhang Yan , Wang Qiuping
Published: February 2023

Objective. To investigate the application value of comprehensive nursing combined with comfort nursing for severe stroke patients with diabetes in the intensive care unit (ICU), as well as its effect on the incidence of pressure ulcers and aspiration. Methods. Between March 2019 and March 2021, 123 severe stroke patients with diabetes who were treated at our hospital were randomly assigned to one of two groups: the control group (n = 61) or the study group (n = 62). The control group received normal care, but the research group received comprehensive nursing as well as comfort nursing. The two patient groups were compared in terms of the effects of the clinical application. Results. The two groups did not differ significantly in general data (P > 0.05). The shorter ICU monitoring and extubation times, the lower incidence of pressure ulcers, aspiration, and nosocomial infections, and higher self‐rating anxiety scale (SAS) and self‐rating depression scale (SDS) scores and a lower MOS 36‐item short‐form health survey (SF‐36) score were all observed in the research group when compared to the control group (P < 0.05). Conclusion. For severe stroke patients with diabetes in the ICU, comprehensive nursing combined with comfort nursing has a promising effect, significantly, lowering the risk of pressure ulcers, aspiration, and nosocomial infections, accelerating physical recovery, enhancing mental state, and ensuring a better prognosis, deserving general clinical promotion.
Catalytic Conversion of Low Alcohol to Hydrocarbons: Challenges, Prospects, and Future Work Considerations
Authors: Anekwe Ifeanyi Michael Smarte , Isa Yusuf Makarfi
Published: February 2023

Energy sources will contribute enormously to global economic development in the coming years. Global energy sectors mainly depend on fossil fuels, which contribute to greenhouse gas emissions. With the threat of declining oil supplies and the need to reduce CO2 emissions, the development of viable substitutes for fossil fuels is high on the global priority list. Currently, there is an increased focus on using low alcohols as fuels and petrochemicals. Catalytic conversion is a viable approach for upgrading low alcohols to fuels and value-added chemicals. This review study shows that optimal catalyst modification improves catalytic performance in the volarization of bioethanol. Heterogeneous catalysts are widely used in bioethanol conversion due to their activity. However, further studies are needed to address the challenges of limited selectivity, catalyst deactivation, recovery, and regeneration for improved biofuel production.
Identification and Pathogenicity of Emerging Fish Pathogen Acinetobacter johnsonii from a Disease Outbreak in Rainbow Trout (Oncorhynchus mykiss)
Authors: Bi Baoliang , Yuan Yin , Jia Dan , Jiang Wansheng , Yan Hui , Yuan Gailing , Gao Yu
Published: February 2023

In November 2017, a group of farmed rainbow trout (Oncorhynchus mykiss) died in Yunnan Province, China, likely due to infection. These rainbow trout exhibited slow movement, no feeding, dark body color, exophthalmia, and occasional ulcers on the body surface. Pathogens were isolated from diseased rainbow trout livers, head kidneys, spleens, and eyes, and the strain was preliminarily identified as Acinetobacter johnsonii based on morphology, biochemical tests, and 16S rRNA and rpoB sequences. The isolated strains were highly sensitive to florfenicol, ciprofloxacin, oxolinic acid, and norfloxacin but resistant to ampicillin, doxycycline, sulfadiazine, thiamphenicol, and sulfamethazine. The selected isolate was performed for the experimental infection of rainbow trout to confirm its pathogenicity. Experimentally infected fish showed disease symptoms similar to those observed in fish naturally infected with these bacteria. Vacuolar degeneration was prevalent in the liver and spleen of diseased fish. Cytoplasm volume was very high in the lymphocytes of the head kidney. The protein structure and topology of monomeric outer membrane protein A (OmpA), outer membrane protein 34 (Omp34), and a nucleoside-specific outer membrane transporter protein Tsx (OmpTsx) of A. johnsonii were predicted by AlphaFold 2. The predicted local distance difference test (pLDDT) score was used for the assessment of structure prediction. Comprehensive analysis of antigenic determinants of the OMP family member, OmpA, contains three antigenic determinants, which are 90% similar with epitopes of A. baumannii. OmpA is a recombinant protein with 35 kDa expressed in the Escherichia coli system. Based on these findings, A. johnsonii is regarded as an emerging opportunistic pathogen in farmed rainbow trout. OmpA protein can be used as a subunit vaccine candidate molecule of A. johnsonii.
The Spectral Adomian Decomposition Method for the Solution of MHD Jeffery–Hamel Problem
Authors: Khidir Ahmed A.
Published: February 2023

In this study, the effects of magnetic field on the Jeffery–Hamel problem is studied using a powerful numerical method called the spectral Adomian decomposition method (SADM). The traditional Navier–Stokes equation of fluid mechanics and Maxwell’s electromagnetism governing equations are reduced to nonlinear ordinary differential equations to model the problem. Comparisons with the numerical solutions are made to demonstrate the validity and high accuracy of the present approach. The velocity profile of the inner part of the divergent channel is studied for various values of magnetic field parameter and angle of channel. It was found that an increase in the magnetic field parameter leads to increase in the velocity profile. The results indicated that this technique is more efficient and converges faster than the standard Adomian decomposition method.
Microporous MOF-5@AC and Cu-BDC@AC Composite Materials for Methane Storage in ANG Technology
Authors: Nikravesh Niloufar Yadavar , Beygzadeh Mojtaba , Adl Mehrdad
Published: February 2023

Novel composites (MOF-5 and Cu-BDC) based on economically activated carbon have been developed to investigate methane adsorption capacity for ANG applications. The composites were synthesized by adding MOFs precursor in two different weight percent (10% and 40%) to commercial activated carbon under solvothermal conditions (110-120°C). The synthesized adsorbents were characterized by FT-IR, XRD, SEM, EDS, and BET techniques to gather information about their crystallinity, morphology, and specific surface area. A methane uptake measurement system based on the volumetric method was made to obtain methane adsorption capacity on each composite. Then, the amount of methane adsorption for each one was calculated, and the experimental data were compared with different isotherm adsorption models appropriate for gas adsorption, including Langmuir, Freundlich, and Dubinin-Radushkevich. This comparison showed that the best fitting belonged to the Langmuir isotherm model. Also, stability and kinetic studies were done for two MOF-540% @AC and Cu-BDC40% @AC composites. In the kinetics study, experimental data were compared and analyzed in terms of pseudo-first order, pseudo-second order, and intraparticle diffusion models. The kinetics study showed that methane adsorption happens very fast on the synthesized adsorbents. The amount of methane adsorption at 35 bar and room temperature for pure activated carbon was specified as 4.32 mmol/g. The 10% and 40% of Cu-BDC with activated carbon are 5.59 mmol/g and 6.85 mmol/g, respectively. The capacity of MOF-510% @AC and MOF-540% @AC composites were obtained at 5.9 mmol/g and 7.3 mmol/g, respectively. Increasing methane uptake (70%) was obtained by adding 40 wt.% of MOF-5 to commercial activated carbon.
The Cost Efficiency of the Electricity Retailers with the Integration of the Cloud Energy Storage
Authors: Xiong Chu , Luo Dan , Han Liang
Published: February 2023

As a result of market liberalisation, a large number of electricity retailers have emerged in the electricity market. Acting as the intermediaries between the electricity producers and the customers, the electricity retailers aim to balance the supply and demand and shoulder substantial risks generated from both sides. Due to the randomness of the electricity load, it is difficult for electricity retailers to make an accurate electricity purchasing plan in advance to meet customer demand. This deviation leads to a proportion of spot electricity purchases that require a higher purchase cost. As a result, one of the most serious concerns facing electricity retailers is how to improve their balancing abilities and reduce power purchase deviation. In contrast to previous research, which has generally recommended that electricity retailers invest in energy storage systems or develop optimised purchasing strategies, this paper proposes a new strategy for the electricity retailers, which is renting external flexible resources to solve the market uncertainty of the electricity retailers, thereby lowering purchase costs and increasing profits. The proposed business model makes use of the cloud energy storage to solve the supply-demand imbalance issue of electricity retailers. The cost calculation model and decision optimisation model have been established in the process of renting cloud energy storage. Charging and discharging cloud energy storage have been separately rented to deal with different positive and negative load deviations, which can simplify the optimisation model. As an experimental paper, the proposed model has been tested in the PJM power market in the United States and the New South Wales power market in Australia. The findings confirm that renting the cloud energy storage capacities can significantly reduce costs and maximise profits for the electricity retailers when compared to the situation without the cloud energy storage. The biggest saving can reach 24.5% in the PJM market. With the rapid fall of battery prices, the advantage of the proposed strategy will be more obvious.
Efficacy and Safety of Integrated Maxing Shigan Decoction and Azithromycin for Mycoplasma Pneumoniae Pneumonia in Children: A Systematic Review and Meta-Analysis
Authors: Huang Qiong , Zhang Qiong , Li Si
Published: February 2023

Background. The primary objective of this study was to thoroughly assess the effectiveness of treating pediatric mycoplasma pneumoniae pneumonia (MPP) with Maxing Shigan decoction (MSD) and azithromycin. Materials and Methods. We comprehensively reviewed the literature for randomized controlled trials research on MPP treated by MSD combined with azithromycin in children in the databases of Medicine, Embase, ClinicalTrials.gov, Cochrane Library, PubMed, CNKI, WangFang, and VIP from inception to April 2021. dds ratio (OR) was used for dichotomous data, while the mean difference (MD) was adopted for continuous variables as effect size, both of which were demonstrated in effect size and 95% confidence intervals (CI). Results. A total of 49 studies with 5704 patients were included in the meta-analysis. The results showed that MSD combined with azithromycin significantly improved the clinical efficacy (OR = 5.31, 95% CI: (4.35, 6.49), and

p
 
<
 
0.001

) and reduced the chest X-rays recovery time (MD = −2.25, 95% CI: (−2.86, −1.65), and

p
 
<
 
0.001

), cough duration (MD = −2.53, 95% CI: (−2.93. −2.12), and

p
 
<
 
0.001

), CRP (MD = −7.84, 95% CI: (−9.51, −6.17), and

p
 
<
 
0.001

), fever duration (MD = −1.53, 95% CI: (−1.78, −1.28), and

p
 
<
 
0.001

), hospital days (MD = −2.70, 95% CI: (−3.35, −2.06), and

p
 
<
 
0.001

), moist rales duration (MD = −2.00, 95% CI: (−2.33, −1.68), and

p
 
<
 
0.001

), and adverse effects (OR = 0.55, 95% CI: (0.41, 0.75), and

p
 
<
 
0.001

) when compared with azithromycin alone in the treatment of MPP in children. Meta-regression analysis demonstrated that the course of disease and duration of medication contribute to the heterogeneity across studies when comparing outcomes between groups. Conclusion. MSD and azithromycin worked better when used in conjunction to treat MPP in young patients. These findings require confirmation by additional high-quality, big sample RCTs.
The Efficacy and Safety of a Qiliqiangxin Capsule Combined with Sacubitril/Valsartan in the Treatment of Chronic Heart Failure: A Systematic Review and Meta‐Analysis
Authors: Chen Wensheng , Chang Huihua , Wang Xiaoqi , Wang Yuanping , Liu Yuntao , Wang Dawei
Published: February 2023

Background. Qiliqiangxin (QLQX) capsules are a commonly used proprietary Chinese medicine for the adjuvant treatment of chronic heart failure (CHF) in China. In recent years, several randomized controlled trials (RCTs) have reported on the efficacy and safety of QLQX combined with sacubitril/valsartan for CHF. Objective. The purpose of this study was to systematically analyze the clinical efficacy and safety of QLQX combined with sacubitril/valsartan in the management of CHF and to provide clinicians as well as scientists with optimal evidence‐based medical evidence. Methods. We searched RCTs to evaluate the efficacy and safety of QLQX combined with sacubitril/valsartan in the treatment of CHF in the Wanfang Database, China National Knowledge Infrastructure, China Science and Technology Journal Database, PubMed, Embase, and Cochrane Library databases from their inception until January 8, 2022. RCTs on QLQX in combination with sacubitril/valsartan for CHF were included. The outcome measures considered were total effective rate, left ventricular ejection fraction (LVEF), left ventricular end‐diastolic dimension (LVEDD), 6‐minute walking distance (6‐MWD), and adverse events. The quality of the included RCTs was assessed thereafter using the Cochrane risk of bias tool. RevMan 5.3 software was used to conduct the meta‐analysis. Results. The meta‐analysis included 17 trials involving 1427 CHF patients. The results indicated that with sacubitril/valsartan administration combined with QLQX treatment, the total effective rate (relative risk (RR) = 1.24; 95% confidence interval (CI) (1.17, 1.31); p < 0.01), LVEF (mean difference (MD) = 6.20; 95% CI (5.36, 7.05; p < 0.01)), and 6‐MWD (MD = 55.87; 95% CI (40.66, 71.09); p < 0.01) of CHF patients were significantly increased, and the LVEDD value of CHF patients was noted to be significantly reduced (MD = −3.98; 95% CI (−4.47, −3.48); p < 0.01). Moreover, there was no increase in the number of adverse events during treatment (RR = 0.67; 95% CI (0.33, 1.34); p < 0.01). Conclusions. This study indicated that in CHF patients, on the basis of sacubitril/valsartan treatment, combination with QLQX can potentially enhance the total effective rate, improve LVEF and 6‐MWD, and reduce LVEDD values, with good safety. However, considering the poor quality of the included studies, a multicenter, randomized, double‐blind controlled study is needed for further confirmation.
Antimicrobial Potential of Pithecellobium dulce Seed Extract against Pathogenic Bacteria: In Silico and In Vitro Evaluation
Authors: Aldarhami Abdu , Bazaid Abdulrahman S. , Alhamed Abdullah S. , Alghaith Adel F. , Ahamad Syed Rizwan , Alassmrry Yasseen A. , Alharazi Talal , Snoussi Mejdi , Qanash Husam , Alamri Abdulwahab , Badraoui Riadh , Kadri Adel , Binsaleh Naif K. , Alreshidi Mousa
Published: February 2023

Clinical multi‐drug‐resistant bacteria continue to be a serious health problem. Plant‐derived molecules are an important source of bioactive compounds to counteract these pathogenic bacteria. In this paper, we studied the chemical composition of the methanol (80%) extract from Pithecellobium dulce seed (Hail, Saudi Arabia) and its ability to inhibit the growth of clinically relevant multi‐drug‐resistant bacteria. Molecular docking analysis was performed to predict the best compounds with low binding energy and high affinity to interact with two Staphylococcus aureus receptors. Data showed that P. dulce extract is a rich source of D‐turanose (55.82%), hexadecanoic acid (11.56%), indole‐1‐acetic acid (11.42%), inositol (5.78%), and octadecanoic acid (4.36%). The obtained extract showed antibacterial activity towards tested clinical bacterial strains with MIC values ranging from 233 mg/mL for Acinetobacter baumannii to 300 mg/mL for S. aureus and Escherichia coli. Turanose interaction has resulted in ‐7.4 and ‐6.6 kcal/mol for 1JIJ and 2XCT macromolecules, while inositol showed energy values (−7.2 and −5.4 kcal/mol) for the same receptors. Multiple identified compounds showed desirable bioavailability properties indicating its great potential therapeutic use in human. Overall, current investigation highlights the possible use of P. dulce extract as a valuable source for drug development against pathogenic drug‐resistant bacteria.
Screening and Analyzing of Genes and Signaling Pathways Associated with Size Differentiation of Adult Male Prawn Macrobrachium nipponense
Authors: Jiang Gang , Zhu Yongming , Xue Yucai , Lu Ying , Liu Zhiwei , Huang Xuxiong
Published: February 2023

To better understand the size differentiation mechanism of adult male prawn Macrobrachium nipponense, transcriptome sequencing was performed from the second pereiopod, hepatopancreas, and muscle of adult male prawn M. nipponense with significant differences in size (large: 1.47 ± 0.12 g; small: 0.28 ± 0.04 g). A total of 46,557 unigenes with an average length of 1,312 bp were obtained. Based on pereiopod of large male (LP) versus pereiopod of small male (SP), muscle of large male (LM) versus muscle of small male (SM), and hepatopancreas of large male (LH) versus hepatopancreas of small male (SH), a total of 805, 473, and 1,037 significantly upregulated DEGs were identified, respectively. The KEGG pathway analysis indicated that pathways such as the PI3K-Akt pathway, the cAMP pathway, the Hippo pathway, and the Ras signaling pathway were enriched in all three comparisons, which may be key size regulators in adult male prawn M. nipponense. Furthermore, as potential candidates for size differentiation in adult male prawns, twenty-eight DEGs involved in these four pathways were screened out, and their expressions were verified by quantitative real-time PCR (qPCR). The results of this study preliminarily elucidate the molecular mechanism of male M. nipponense in size differentiation and provide a theoretical reference for improving the commercial size of cultured male prawn M. nipponense.
[Retracted] Applications of Neural Network‐Based Plan‐Cancer Method for Primary Diagnosis of Mesothelioma Cancer
Authors: Kapila Dhiraj , Panwar Sarika , Raja M. K. Mohan Maruga , Mondal Tamal , Rafi Shaik Mohammad , Singh Suryabhan Pratap , Kumar Bhupendra
Published: February 2023

“Malignant mesothelioma (MM)” is an uncommon although fatal form of cancer. The proper MM diagnosis is crucial for efficient therapy and has significant medicolegal implications. Asbestos is a carcinogenic material that poses a health risk to humans. One of the most severe types of cancer induced by asbestos is “malignant mesothelioma.” Prolonged shortness of breath and continuous pain are the most typical symptoms of the condition. The importance of early treatment and diagnosis cannot be overstated. The combination “epithelial/mesenchymal appearance of MM,” however, makes a definite diagnosis difficult. This study is aimed at developing a deep learning system for medical diagnosis MM automatically. Otherwise, the sickness might cause patients to succumb to death in a short amount of time. Various forms of artificial intelligence algorithms for successful “Malignant Mesothelioma illness” identification are explored in this research. In relation to the concept of traditional machine learning, the techniques support “Vector Machine, Neural Network, and Decision Tree” are chosen. SPSS has been used to analyze the result regarding the applications of Neural Network helps to diagnose MM.
Eco-Environmental Uncertain Management of Various Energy Hubs within a Microgrid Concentrated on Demand Response, Intelligent Parking Lots, and Power-to-Gas Unit Effects
Authors: Gharai Majid Abbasi , Hooshmand Rahmat-Allah , Nosratabadi Seyyed Mostafa
Published: February 2023

Nowadays, consumption of different energy carriers is increasing due to the division of community activities in various sectors such as residential, commercial, and industrial. The energy hub concept is used to meet the demands of different energy carriers in these sections. In this paper, a new method for energy management of a microgrid is presented in an intelligent network based on three types of commercial, residential, and industrial energy hubs. In the energy hubs, a wide range of components, including renewable and nonrenewable generation units, converters, storage devices, intelligent parking lots consisting of electric vehicles, P2G units, and cogeneration units, are used to supply electrical, heating, cooling, and natural gas energies. Some parameters like renewable generations, energy demands, and arriving and departure time of electric vehicles are considered to be uncertain, and a relevant method is applied to provide a near-realistic profile for them. In addition, a demand response paradigm has also been proposed for three types of electrical, heating, and cooling demands at the hub output side. Therefore, in this method, a mixed integer linear programming model is proposed based on benefit and reduction of emission caused by the activity of gas-burning units for short-term planning and obtaining an optimal solution for generation and sending loads in a distribution network containing energy hubs. To evaluate the performance of the proposed modeling and structure, the presented approach is applied on a modified 33-bus IEEE test network. According to the results of the energy management model, it is possible to significantly increase benefits and also obtain a smoother consumption pattern in consumption with financial incentives.
Efficacy of Fufang E’jiao Jiang in the Treatment of Patients with Qi and Blood Deficiency Syndrome: A Real‐World Prospective Multicenter Study with a Patient Registry
Authors: Zhao Mengjie , Xiao Mengli , Ying Jiake , Qiu Panbo , Wu Hongxian , Tan Qin , Chen Shiliang , Zhang Lulu , Shi Sanfei , Li Guida , Zhao Yang , Lu Fang
Published: February 2023

Objective. This nationwide, multicenter prospective observational study with a patient registry was designed to evaluate the efficacy of Fufang E’jiao Jiang (FEJ) in Chinese patients with Qi and blood deficiency syndrome (QBDS). Methods. QBDS patients were consecutively recruited from 81 investigational sites in China from July, 2019, to December, 2020. Patients who met the eligibility criteria were enrolled in a prospective registry database. Baseline characteristics and changes in scores on the traditional Chinese medicine (TCM) symptom evaluation scale for Qi and blood deficiency, the clinical global impression (CGI) scale, the fatigue scale‐14 (FS‐14), and the Pittsburgh sleep quality index (PSQI) were analyzed to determine the clinical efficacy of FEJ. Results. A total of 3,203 patients were recruited. The average remission rate (i.e., the sum of the cure rate and improvement rate) of the 20 symptoms of QBDS was 92.49% after 4 weeks of FEJ treatment, which was higher than at baseline; the rate increased to 94.69% at 8 weeks. The CGI scale revealed that the number of total remissions at 4 and 8 weeks was 3,120 (97.41%) and 415 (100%), respectively. The total FS‐14 scores decreased by 1.67 ± 4.11 (p < 0.001) at 4 weeks and 1.72 ± 3.09 (p < 0.001) at 8 weeks of treatment. The PSQI scores were 6.6 ± 4.7 and 6.52 ± 3.07 at 4 and 8 weeks, respectively, which were significantly lower than the baseline scores (p < 0.001; p = 0.0033). Both the subhealth fatigue (SF) and iron deficiency anemia (IDA) groups showed significantly improved clinical symptoms of QBDS (p < 0.01). Between‐group comparisons revealed significantly greater improvements in FS‐14 and PSQI scores in the SF group than in the IDA group (p < 0.05). A multivariate logistic regression analysis showed that disease course, FS‐14 score at baseline, and four‐week FEJ doses were independent risk factors for the degree of symptom relief in QBDS patients (p < 0.05). Conclusion. In real‐world settings, FEJ has a promising effect in treating QBDS and can significantly improve the severity of its symptoms.
A Review of Solar Photovoltaic Power Utilizations in India and Impacts of Segregation and Safe Disposal of Toxic Components from Retired Solar Panels
Authors: Jayapradha P. , Barik Debabrata
Published: February 2023

Environmental impacts of electricity production through nonrenewable sources are greatly reduced by solar energy production through PV cells. The use of solar energy as an alternative to conventional methods is about to increase tenfold by the year 2050. This considerably increases the number of solar cell wastes for which the recycling processes are confronted with various issues due to the presence of hazardous materials like Al, lead, chromium, glass, silver, and ethylene vinyl. Different approaches for the disposal and segregation of these materials through mechanical, thermal, and chemical means are investigated in this review work. Since many countries have begun to implement mechanisms to deal with the destruction of solar PV (photovoltaic) panels, this evaluation will concentrate on the existing mechanisms and procedures. Existing mechanisms like landfilling, open dumping, and regulations and policies of the retired solar panel were discussed.
Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial Cells
Authors: Yin Hao , Zhu Jiaoli , Jiang Yi , Mao Yijing , Tang Chenquan , Cao Hui , Huang Yufang , Zhu Huijun , Luo Jianping , Jin Qingjiang , Jin Qinglei , Xue Yi , Wang Xin
Published: February 2023

In clinical practice, urinary tract infections (UTIs) are second only to respiratory infections in terms of infectious diseases. In recent years, drug resistance of Escherichia coli (E. coli) has increased significantly. The therapeutic effects of Shionone on UTI were assessed by modelling UTI in SD rats and SV-HUC-1 cells with E. coli solution. After treatment of Shionone, the UTI rat model showed a decrease in wet weight/body weight of bladder, as well as a reduction in cellular inflammatory infiltration of bladder tissue and a decrease in urinary levels of IL-6, IL-1β, and TNF-α. In addition, the levels of proinflammatory factors were significantly reduced in a dose-dependent manner in UTI cell model treated with different doses of Shionone (5, 10, and 20 μg/kg). The results of immunofluorescence analysis in both in vivo and in vitro experiments revealed that Shionone reduced bacterial load and the number of E. coli colonies growing on the plates was greatly reduced. These results suggested that Shionone has a good therapeutic effect on UTI, achieved by reducing bacterial load in bladder epithelial cells. The data presented here provide a basis for further research into the treatment of UTI.
MBNL1-AS1 Promotes Hypoxia-Induced Myocardial Infarction via the miR-132-3p/RAB14/CAMTA1 Axis
Authors: Li Yanbing , Zong Min , Guan Xiaonan , Wu Xuejiao , Ma Guiling , Wei Yu , Li Zhi
Published: February 2023

Background. Mounting evidence have indicated that long noncoding RNA (lncRNA) muscleblind like splicing regulator 1 antisense RNA 1 (MBNL1-AS1) play a crucial regulatory role in cardiovascular disease, myocardial infarction (MI) included. In this research, we sought to probe into the biological function and potential mechanism of MBNL1-AS1 in MI. Methods. Cardiomyocytes were treated under hypoxic conditions for 0–12 h. Functional assays including CCK-8 and flow cytometry were performed to assess hypoxia-stimulated cardiomyocyte viability and apoptosis, respectively. Moreover, bioinformatics analysis and mechanical assays were conducted to reveal the competitive endogenous RNA (ceRNA) mechanism of MBNL1-AS1. Results. The upregulation of MBNL1-AS1 was found in hypoxia-stimulated cardiomyocytes. Functionally, the downregulation of MBNL1-AS1 dramatically promoted hypoxia-induced cardiomyocyte viability and inhibited apoptosis. Mechanistically, miR-132-3p bound to MBNL1-AS1 in hypoxia-induced cardiomyocytes, and miR-132-3p directly targeted RAB14, member RAS oncogene family (RAB14) and calmodulin binding transcription activator 1 (CAMTA1). Furthermore, MBNL1-AS1 upregulates the expression of RAB14 and CAMTA1 in hypoxia-stimulated cardiomyocytes via targeting miR-132-3p. Conclusions. The current study revealed the critical role of the MBNL1-AS1/miR-132-3p/RAB14/CAMTA1 axis in MI, indicating MBNL1-AS1 as an innovative therapeutic target for MI.
The Influence of Smartphone Screen Time on Acquired Premature Ejaculation: A Prospective Preliminary Study
Authors: Cinislioglu Ahmet Emre , Demirdogen Saban Oguz , Utlu Adem , Aksakallı Tugay , Altay Mehmet Sefa , Akkas Fatih , Karabulut Ibrahim , Ozbey Isa
Published: February 2023

This prospective study is aimed at investigating the relationship between smartphone screen time and the intravaginal ejaculation latency time (IELT) in patients with acquired PE and to contribute to the literature. Thirty patients who had been diagnosed with acquired PE in our clinic between March 2022 and May 2022 were included in the study. Carrying similar demographic characteristics to the patient group, a total of 30 healthy volunteers were included as the control group. Patients who were using Huawei or iPhone brand of smartphones and who had data of at least one week of screen time were included in the study. The data were collected from the applications on the phones. PE was evaluated using PEDT, the Turkish validation of which had been carried out. The patients and the controls also underwent the application of the Quality-of-Life Questionnaire-Short Form (SF-36), which has also been validated for Turkish. The patients and the controls were compared with regard to smartphone screen time, IELT, PEDT scores, and the SF-36 scores. The mean screen time was found to be statistically significantly higher in the PE group compared to that of controls (

336
±
137
 
min
/
day

,

246
±
76.4
 
min
/
day

, respectively,

p
=
0.045

). In the correlation analysis between the IELT and the PEDT scores of the patients, a significant negative correlation was found between the screen time and the IELT, and a significant positive correlation was found between the screen time and the mean PEDT scores (

p
<
0.001

,

p
<
0.001

, respectively). Our results showed that smartphone screen time negatively affected the IELT values and PE severity in patients with acquired PE.
Response Analysis of a Ballasted Rail Track Constructed on Soft Soil by 3D Modeling
Authors: Hoque Iram Lamiya , Aziz Shamontee , Mallick Joya Rani , Nayeem Md. Arifuzzaman , Maisha Tamanna-E-Hafiz , Hossain F. M. Zahid , Amin A. F. M. S.
Published: February 2023

The displacement, stress, and strain distributions of railway embankments on the soft deltaic deposit of the Ganges–Brahmaputra floodplain are investigated. A numerical model developed in general-purpose finite element software is used to simulate the design train load on a deltaic deposit for a 100 km/hr rail speed. The numerical analysis analogy is grounded in the spring model, where a beam under the unit load is modeled based on the Winkler foundation model concept. In the moving load simulation on soil, the static point load relating to the axle load is assigned in the form of a dynamic multiplier, determined using auxiliary software. The calculated shear force in terms of the influence line is applied as a dynamic multiplier. The numerical results demonstrate that under a dynamic train load, the loose ballast undergoes larger and more erratic displacement than the subballast. Comparative analysis between varying subballast stiffnesses shows that stiffer subballast yields smaller displacements. Moreover, a high subballast stiffness can counterbalance the potential of forming permanent deformation by generating lower strains. However, a stiffer subballast does not play a prominent role in reducing the displacement of ballast or vertical stresses. The subgrade is found to carry the maximum load, withstanding the maximum vertical stress; thus, the importance of using an improved subgrade with higher stiffness is also observed. A greater subgrade stiffness improves its load-carrying capacity but fails to reduce the tension responsible for the lateral spreading of the soft subsoil. To reduce the high radial strain, the effects of improving the stiffness properties of two immediately adjacent soft soil layers are numerically investigated. The improvement of subsoil alone is effective in reducing the radial strain, whereas the improvement of both subgrade and subsoil produces further reductions. The critical train speed generating the maximum displacement is identified as 120 km/hr, and the dynamic velocity amplitude decreases with depth. Finally, an allowable limit of rail embankment settlement on a soft deltaic deposit is observed.
YB-1 Expression Is Associated with Lymph Node Metastasis and Drug Resistance to Adriamycin in Breast Cancer
Authors: Dong Hanzhi , Jian Yan , Yu Tenghua , Wang Meijian , Zhang Wenfeng , Peng Zhiqiang
Published: February 2023

Background. Breast cancer (BC) is the most common malignant tumor among females. Although there are multiple treatments for breast cancer, many patients still face the dilemma of drug resistance after multiline treatment. It would be greatly helpful for clinical work to identify additional and improved prognostic predictors. Y-box binding protein-1 (YB-1) is a member of the cold shock protein family, and patients with overexpression of YB-1 have a worse prognosis. Methods. This study collected 48 specimens from 48 patients with breast cancer and analyzed the clinicopathological characteristics of the patients. Immunohistochemistry, immunofluorescence, cell viability analysis, tumor spheroid formation and cell morphology, cell invasion, cycle analysis, qRT-PCR, Western blot, and tumorigenicity in BALB/c nude mice were performed to verify the results. Results. We found that patients with overexpression of YB-1 were related to lymph node metastasis and the patients’ age tended to be young. Because of the short follow-up time, a survival analysis could not be performed. Based on the results of in vitro and in vivo experiments, this study indicated that breast cancer cells with overexpression of YB-1 had stronger proliferation, migration, and invasion abilities than cells with low expression of YB-1. Compared with cells with low expression of YB-1, the proliferation, migration, and invasion abilities of YB-1 overexpressed cells were not significantly affected by adriamycin. Conclusion. This suggested that breast cancer cells with overexpression of YB-1 were resistant to adriamycin. Therefore, YB-1 is associated with lymph node metastasis of breast cancer cell. YB-1 could be a prognostic, predictive factor and a novel therapeutic target of BC.
Multiple Impacts of Urban Built and Natural Environment on Lung Cancer Incidence: A Case Study in Bengbu
Authors: Gu Kangkang , Li Yuwei , Jia Xianjie , Liu Chengrong
Published: February 2023

Tumours are the main disease affecting the health of the Chinese population, and lung cancer is the malignancy with the highest incidence. Hence, the need to study and analyse the population of lung cancer incidence in order to effectively control and prevent it. In this research, we discuss the demographic characteristics of lung cancer incidence population of 2014 to 2020 from the perspective of multiple urban environmental factors, taking Bengbu city in the Huaihe River Basin of China as the research area, analyse the correlation between environmental indicators and lung cancer incidence population through the Spearman’s rank correlation assessment model, and analyse the interaction between the influence factors of a geographic detector to analyse the influence of urban environmental factors. The results showed the followings: (1) The distribution characteristics of lung cancer incidence population were mainly geriatric population and spatially mainly fell in the old urban area of the study area, and the population distribution had clustered characteristics. (2) Through Spearman’s rank correlation analysis, the land use, road traffic, spatial form, service facilities, and the open space of green space of the urban‐built environment as well as the natural environment are all correlated with the incidence of lung cancer. (3) Factor detection and interaction analysis revealed a greater effect of spring and winter on lung cancer prevalence. In addition, the road intersection density and the distance to industrial are the most important potential influencing factors, and the interaction of any two factors will increase the risk of lung cancer.
The Optimal Development Strategy Selected for Layered Sandstone Reservoir by Creating a System Economic Model
Authors: Wang Fulin , Zhao Yunfei , Fang Yanjun
Published: February 2023

To determine the development mode of layered sandstone reservoir, a system economic model was established, which thought the layer in production and its upper (downer) layer as a system, and the overall benefits of the two modes were contrasted using the existing well (UEWM) or the method of drilling a new well (DNWM); then, the upper (downer) oil layer development mode could be determined quickly by the model. The method can avoid the chemical agent effect of the upper target layer, which also makes the calculation more accurate. In the model, the cumulative oil production limits of the producing layer are described at different oil prices of DNWM and UEWM methods. Then, an economic ultimate water cut (EUWC) model is established. According to this model, when the water cut attains to EUWC, the oilfield should be abandoned. Meantime, the arctangent prediction model for water cut is combined with the water drive curve method, to forecast the accumulative oil from the planning time of the upper target layer production to the abandonment time of the first production layer of oilfield. And then, the development mode could be decided through contrast with the cumulative oil production limit. When the oil price is 40$/bbl, the EUWC of the block is 97.55%, and time is May 2020, earlier than the planned upper layer production time January 2023, so its rest cumulative production would be

0.0
×

10

4

 
t

, which is less than the cumulative production limit of

32
×

10

4

 
t

. Hence, the UEWM should be chosen. When the oil price is $70/bbl, EUWC is 98.93%, and the corresponding time is August 2027. From the planned return time of January 2023 to August 2027, the block has cumulative oil production of

25.54
×

10

4

 
t

, greater than the cumulative oil limitation of

10.8
×

10

4

 
t

. The old exiting well still has larger economic productivity; hence, the DNWM method should be chosen. This work provides theoretical support for up (down) formation development mode of production layer. It is of great significance to guide the development of layered sandstone reservoirs. The system economic model established can decide development mode with drilling wells or using exiting well, and it was first created in the field of oil reservoirs. This method fills up the theoretical blank of development mode for upper or downer layer of the producing layer in layered sandstone reservoir.
Construction of Bimetallic Co/Fe-Incorporated PTA/FDA Nanoclusters for Boosting Electrocatalytic Oxygen Evolution
Authors: Gai Yuping , Hu Junbo , Deng Wei , Xie Wenshuo , Li Xiang , Zhang Jingjing , Long Dewu , Qiao Shanlin , Jiang Fei
Published: February 2023

Summary. Electrochemical water decomposition as a crucial approach for the gradual growth of renewable energy has attracted extensive attention. Metal-organic frameworks (MOFs) which benefit from ultra-high specific surface area, controllable nanostructures, and excellent porosity have been widely used as high activity catalyst for the decomposition of water by electrochemical means. Herein, the composition and morphology of metal–organic framework nanoclusters with bimetallic Co/Fe-incorporated PTA/FDA nanoclusters is designed for efficient and durable OER electrocatalysts, including CoFe-BTC/PTA, CoFe-BTC/FDA, and CoFe-PTA/FDA. The crystal structure of MOF materials is composed of alternating organic hydrocarbon BTC, PTA, or FDA and inorganic metal oxide layer. Co and Fe interact as central atoms, joining BTC, PTA, or FDA ligands to form a highly symmetric MOF structure. The electronic structures and active sites of various metals are different, and the insertion of iron atoms plays a certain role in the regulation of their electronic structures. CoFe-PTA/FDA shows significant OER overpotential

η

10

=
295
 
mV

(1.525 V vs. RHE) reached 10 mA cm-2, with 62.85 mV dec-1 for Tafel slope and pretty conspicuous stability (72 hours of continuous testing). The DFT calculation results show coordination unsaturated metal atom is the primary active center of these electrocatalytic reaction, and the coupling effect caused by adding Fe is the key to adjust the electrocatalytic activity.
The Interaction of Apelin and FGFR1 Ameliorated the Kidney Fibrosis through Suppression of TGFβ-Induced Endothelial-to-Mesenchymal Transition
Authors: Gao Rongfen , Wu Yumin , Yang Qian , Chen Liangdong , Chen Jiwei , Wang Boyong , Liu Zeming , Jin Juan , Li Jinpeng , Wu Gaosong
Published: February 2023

Both epithelial-to-mesenchymal (EMT) and endothelial-to-mesenchymal (EndMT) transitions have shown to contribute to the development and progression of kidney fibrosis. It has been reported that apelin, a regulatory peptide, alleviates EMT by inhibiting the transforming growth factor β (TGFβ) pathway in renal diseases. Additionally, fibroblast growth factor receptor 1 (FGFR1) has been shown to be a key inhibitor of EndMT through suppression of the TGFβ/Smad pathway. In this study, we found that apelin and FGFR1 were spatially close to each other and that the apelin and FGFR1 complex displayed inhibitory effects on TGFβ/Smad signaling as well as associated EndMT in diabetic kidney fibrosis. In cultured human dermal microvascular endothelial cells (HMVECs), we found that the anti-EndMT and anti-TGFβ/Smad effects of apelin were dampened in FGFR1-deficient cells. Either siRNA- or an inhibitor-mediated deficiency of apelin induced the Smad3 phosphorylation and EndMT. Streptozotocin-induced CD-1 diabetic mice displayed EndMT and associated kidney fibrosis, which were restored by apelin treatment. The medium from apelin-deficient endothelial cells stimulated TGFβ/Smad-dependent EMT in cultured HK2 cells. In addition, depletion of apelin and the FGFR1 complex impaired CEBPA expression, and TGFβ-induced repression of CEBPA expression contributed to the initiation of EndMT in the endothelium. Collectively, these findings revealed that the interaction between apelin and FGFR1 displayed renoprotective potential through suppression of the TGFβ/Smad/CEBPA-mediated EndMT/EMT pathways.
Prospect of Hydroxyl Radical Exposure during Seawater Bathing to Treat Amoebic Gill Disease in Atlantic Salmon
Authors: Mahbub Parvez , Yeager Thomas
Published: February 2023

This study aims to undertake hydroxyl (.OH) radical-based preliminary investigations with a view to utilising seawater as a viable alternative to freshwater for the treatment of amoebic gill disease (AGD) in aquaculture industries. The study presents in vitro viability studies of clonal amoebae species to examine the effects of .OH radicals on both parasites and hosts. The study also assesses the toxicity to Chinook salmon cell lines (CHSE-214) in freshwater and 35 ppt seawater via continuous dosing of 35 mM .OH radicals and hydrogen peroxide (H2O2) for 1.5 to 4 hr, separately at 18°C and 15°C. Comparatively high viability of CHSE-214 (60% in .OH and 50% in H2O2) for a prolonged treatment of up to 4 hr in seawater at 15°C indicates suitability of low seawater temperature while using either .OH or H2O2 during bathing. The viability of CHSE-214 remained relatively stable in seawater (55%–60% in .OH and 50%–60% in H2O2), at both temperatures of 18°C and 15°C. However, at 15°C, a drastic reduction of viability of CHSE-214 in freshwater (from 80% to 48% in .OH and from 70% to 58% in H2O2) has indicated high variations in toxicity levels in freshwater at low temperature. Using DNA staining agents in flow cytometry, the in vitro viability study results in >22.5% mortality of clonal Neoparamoeba perurans (NP), an AGD causative agent, in 35 ppt seawater containing 35 mM .OH radicals via one-off dosing for 1 hour at 15°C. In addition, fast radical consumption is more pronounced in the case of .OH radicals as compared to H2O2 in both freshwater and seawater due to extreme reactivity of the former. Hence, this study suggests that .OH radicals are detrimental to the viability of NP in seawater, and thereby, establishes grounds for further in vivo investigations of using seawater supplemented with continuous dosing of .OH radicals for Atlantic salmon bathing as a treatment of AGD.
Biological Reduction of Perchlorate from Spent Modified Reed Mediated by an Enriched Microbial Culture
Authors: Baidas Salem , Al-Deyain Khaled , Meng Xiaoguang , Gao Baoyu
Published: February 2023

Disposal of untreated perchlorate-laden modified reed (MR) generated in the treatment of contaminated water risks further contamination issues. The primary aim of this study was to assess the enriched microbial culture (EMC)-mediated biodegradation of perchlorate bound to spent quaternary amine-MR adsorbent. The culture was enriched to utilize perchlorate as an electron acceptor and brewer’s yeast as an electron donor. Kinetics experiments were performed to determine biodegradation rates and the total time for complete removal. The secondary aim of this research was the development and testing of a microwave-assisted extraction (MAE)-facilitated accurate perchlorate quantitation method. Extraction efficiency was optimized by adjusting the nitric acid concentration, resulting in 90 ± 5% recovery. Results show that EMC can degrade aqueous perchlorate effectively and consistently. EMC can also effectively degrade adsorbed perchlorate from spent MR. In conclusion, anaerobic biodegradation of perchlorate from spent MR can be used as an efficient, cost-effective, and environmentally friendly alternative or supplement to other treatment options, such as ion exchange and incineration. This approach can also be an effective solution to the waste generated by using MR to remove perchlorate from contaminated water.
An Experimental Study with Model Calibration for the Permanent Strain of Granite Residual Soil Subgrade under Drying-Wetting Cycles
Authors: Yang Xuefei , Chen Dongxia , Liu Yue , Zhang Sheng
Published: February 2023

The permanent strain is one of the most important dynamic properties of the granite residual soil. To systematically study the dynamic deformation characteristics of granite residual soil (GRS) under the drying-wetting (D-W) cycles, a series of dynamic triaxial tests is carried out to obtain the stress-strain relationship of GRS with various fine particle contents subjected to D-W cycles. The experimental results show that the growth of permanent strain consists of two stages, namely, compaction stage and compression stage. The main effect of D-W cycles is reflected in the compression stage. On the other hand, the influence of fine particle contents on the permanent strain occurs mainly at the compaction stage, which can be classified as the lubrication, densification, and asphyxiation, respectively. Subsequently, a new criterion to characterize the permanent strain of GRS is deduced from the experimental results, which accounts for both strains and rates of strains. This criterion illustrates that with the increase of D-W cycles number, the permanent strain evaluation pattern of GRS is gradually changing from plastic stabilization to plastic creep. Finally, a quadratic function model referring to the log-log scale is proposed to fit well the relationship between the permanent strain and loading cycle number of GRS under D-W cycles. It is found that the parameters of the proposed model can physically represent the strain rate, the compression strain, and the compaction strain.