bluuebunny/crossref_metadata_2025 huggingface dataset:
Psychological Stress of Parents of Children With Atopic Dermatitis: The Korea National Health and Nutrition Examination Survey
Authors: Lee Hyun Ji , Lee Gyu-Na , Lee Ji Hyun , Han Ju Hee , Han Kyungdo , Park Young Min
Published: October 2021

Background: Atopic dermatitis (AD) is a chronically relapsing inflammatory skin condition that has profound impacts on patient and family quality of life.Objectives: To investigate the psychological stress of parents of children with AD in Korea using data from the Korean National Health and Nutrition Examination Survey (KNHANES).Methods: The cross-sectional study included parents of 8,575 participants under age 19 (970 with AD and 5,733 without AD) from the 2009–2012 KNHANES. Self-perception of stress, depressed mood, suicidal ideation, and diagnosis of depression by a physician were assessed for determination of psychological distress. Results: After adjusting for age, gender, education level, occupation, and marital status, logistic regression analyses indicated that mothers of children with AD showed higher frequency of stress perception (adjusted odds ratio [aOR] 1.458 [95% confidence interval (CI), 1.223-1.739], p < 0.0001) and suicide ideation (aOR 1.403 [95% CI 1.099-1.791], p = 0.0066) than those without AD. In contrast, fathers of children with AD did not show a significant difference compared to those of children without AD.Conclusions: Understanding the psychological stress of parents of children with AD is important for clinicians as evaluation, management, and support for parents, especially mothers, of children with AD are required.
Ketogenic Diet Ameliorates Lipid Dysregulation in Type 2 Diabetic Mice by Downregulating Hepatic Pescadillo 1
Authors: Zhou Jielin , Lu Yao , Jia Yajing , Lu Jing , Jiang Zhengxuan , Chen Keyang
Published: October 2021

Background: Previous reports implied a possible link between PES1 and lipid metabolism. However, the role of PES1 in regulation of T2DM related lipid metabolism and the effect of KD on PES1 have not been reported. The aim of present study is to explore the role of PES1 in effects of ketogenic diet (KD) on diabetic mice and its mediated mechanism.Methods: Male C57BL/6J and KKAy mice were fed with standard diet (SD) and KD, respectively. Simultaneously, McArdle 7777 cells were treated by β-hydroxybutyric acid (β-HB), Pes1 siRNA or Pes1 overexpression plasmid, respectively. Additionally, liver-conditional knockout (CKO) of Pes1 in vivo were used.Results: We unexpectedly found that hepatic PES1 expression in T2DM patients was markedly elevated, but the elevated PES1 was suppressed by KD feeding in T2DM mice with the reduction of hepatic and plasma triglycerides (TG). In mice with CKO of Pes1, the protein levels of p300, SREBP1c, FASN, SCD1, caspase1, NLRP3 and GSDMD were dramatically downregulated in livers, and the plasma and hepatic TG, IL-1β and IL-18 were decreased as well. The similar phenomena were also observed in β-HB and Pes1 knockdown treated hepatocytes. By contrast, Pes1 overexpression in cultured hepatocytes showed that these levels were significantly enhanced, which however were reduced under β-HB treatment. Mechanistically, we discovered that β-HB decreased CHOP binding to the Pes1 promoters, resulting in the downregulation of PES1, thereby reducing PES1 binding to p300 and caspase1 promoters. The inhibition of p300 and caspase1 expressions elicited the dramatic suppression of acetylation of SREBP1c via its interaction with p300, and the decreased GSDMD levels. Besides, knockdown of caspase1 also alleviated the TG levels in cultured hepatocytes.Conclusion: KD may improve lipid dysregulation in type 2 diabetic mice by downregulating hepatic PES1 expression.
Simultaneous Determination of Eight New Generation Amide Insecticide Residues in Complex Matrix Agri-food by Multi-walled Carbon Nanotube Cleanup and UPLC-MS/MS
Authors: Lin Tao , Chen Xinglian , Wang Li , Fang Haixian , Li Maoxuan , Li Yangang , Liu Hongcheng
Published: October 2021

The simultaneous determination method of 8 amide pesticides by multi-walled carbon nanotubes (MWCNs) cleanup, combined with QuEChERS method and ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry has been developed and successfully applied in complex matrix such as green onions, celery, leeks, citrus, lychees, avocado. The matric effect of MWCNs is optimized and compared with QuEChERS materials. The results show that MWCNs can effectively reduce the matrix effect in sample extraction. The mass spectrometry is optimized, through their chemical structure skeletons, the ESI+ and ESI- mode are simultaneously scanned in the method. The coefficient (r) is greater than 0.9990, the limit of quantification ranges from 0.03 to 0.80 μg/kg, the recovery rate ranges from 71.2% to 120%, and the relative standard deviation (RSD) ranges from 3.8% to 9.4%. The method is fast, simple, sensitive, and has good purification effect. It is suitable for the rapid determination of amide pesticides in complex matrix agri-food.
A universal framework for featurization of atomistic systems
Authors: Lei Xiangyun , Medford Andrew
Published: October 2021

Molecular dynamics simulations are an invaluable tool in numerous scientific fields. However, the ubiquitous classical force fields cannot describe reactive systems, and quantum molecular dynamics are too computationally demanding to treat large systems or long timescales. Reactive force fields based on physics or machine learning can be used to bridge the gap in time and length scales, but these force fields require substantial effort to construct and are highly specific to a given chemical composition and application. A significant limitation of machine learning models is the use of element-specific features, leading to models that scale poorly with the number of elements. This work introduces the Gaussian multipole (GMP) featurization scheme that utilizes physically-relevant multipole expansions of the electron density around atoms to yield feature vectors that interpolate between element types and have a fixed dimension regardless of the number of elements present. We combine GMP with neural networks to directly compare it to the widely used Behler-Parinello symmetry functions for the MD17 dataset, revealing that it exhibits improved accuracy and computational efficiency. Further, we demonstrate that GMP-based models can achieve chemical accuracy for the QM9 dataset, and their accuracy remains reasonable even when extrapolating to new elements. Finally, we test GMP-based models for the Open Catalysis Project (OCP) dataset, revealing comparable performance to graph convolutional deep learning models. The results indicate that this featurization scheme fills a critical gap in the construction of efficient and transferable machine-learned force fields.
Base Editing of Human Pluripotent Stem Cells for modeling Long QT syndrome
Authors: Wu Fujian , Guo Tianwei , Sun Lixiang , Li Furong , Yang Xiaofei
Published: October 2021

Human pluripotent stem cells (hPSCs) have great potential for disease modeling, drug discovery, and regenerative medicine as they can differentiate into many different functional cell types via directed differentiation. However, the application of disease modeling is limited due to a time-consuming and labor-intensive process of introducing known pathogenic mutations into hPSCs. Base editing is a newly developed technology that enables the facile introduction of point mutations into specific loci within the genome of living cells without unwanted genome injured. We describe an optimized stepwise protocol to introduce disease-specific mutations of long QT syndrome (LQTs) into hPSCs. We highlight technical issues, especially those associated with introducing a point mutation to obtain isogenic hPSCs without inserting any resistance cassette and reproducible cardiomyocyte differentiation. Based on the protocol, we succeeded in getting hPSCs carrying LQTs pathogenic mutation with excellent efficiency (21.7 % of heterozygous clones, 9.1 % of homozygous clones) in less than 20 days. In addition, we also provide protocols to analyze electrophysiological of hPSC-derived cardiomyocytes using multi-electrode arrays. This protocol is also applicable to introduce other disease-specific mutations into hPSCs.
Ultrafast Demagnetization Excited by Extreme Ultraviolet Light From a Free-Electron Laser
Authors: Philippi-Kobs André , Müller Leonard , Berntsen Magnus , Roseker Wojciech , Riepp Matthias , Bagschik Kai , Wagner Jochen , Frömter Robert , Danailov Miltcho , Capotondi Flavio , Pedersoli Emanuele , Manfredda Michele , Kiskinova Maya , Stransky Michal , Lipp Vladimir , Scherz Andreas , Ziaja Beata , Oepen Hans , Grübel Gerhard
Published: October 2021

Ultrashort and intense extreme ultraviolet (XUV) and X-ray pulses readily available at free-electron lasers (FELs) enable studying non-linear light−matter interactions on femtosecond timescales. Here, we report on the non-linear fluence dependence of magnetic scattering of Co/Pt multilayers, using FERMI FEL’s 70-fs-long single and double XUV pulses, the latter with a temporal separation of 200 fs, with a photon energy slightly detuned to the Co M2,3 absorption edge. We observe a quenching in magnetic scattering that sets-in already in the non-destructive fluence regime of a few mJ/cm² typically used for FEL-probe experiments on magnetic materials. Calculations of the transient electronic structure in tandem with a phenomenological modeling of the experimental data by means of ultrafast demagnetization unambiguously show that XUV-radiation-induced demagnetization is the dominant mechanism for the quenching in the investigated fluence regime of <50 mJ/cm², while light-induced changes of the electronic core levels are predicted to additionally occur at higher fluences. The modeling of the data further indicates that the demagnetization proceeds on the sub-20-fs timescale. This ultrashort timescale is consistent with non-coherent models for ultrafast demagnetization, considering the sub-femtosecond lifetime of hot electrons with energies of a few 10 eV generated by the XUV radiation.
Successful Management of a 29-Day-Old Infant with Severe Hyperlipidemia from a Novel Homozygous Variant of GPIHBP1 Gene
Authors: Liu Shu , Wang Zhiqing , Zheng Xianhua , Zhang Ye , Wei Sisi , OuYang Haimei , Liang Jinqun , Chen Nuan , Zeng Weihong , Jiang Jianhui
Published: October 2021

Background: Severe hyperlipidemia is characterized by markedly elevated blood triglyceride levels and severe early-onset cardiovascular diseases, pancreatitis, pancreatic necrosis or persistent multiple organ failure if left untreated. It is a rare autosomal recessive metabolic disorder originated from the variants of lipoprotein lipase gene, and previous studies have demonstrated that most cases with severe hyperlipidemia are closely related to the variants of some key genes for lipolysis, such as LPL, APOC2, APOA5, LMF1 and GPIHBP1. Meanwhile, other unidentified causes also exist and are equally worthy of attention.Methods: The 29-day-old infant was diagnosed with severe hyperlipidemia, registering a plasma triglyceride level as high as 25.46 mmol/L. Whole exome sequencing was conducted to explore the possible pathogenic gene variants for this patient.Results: The infant was put on a low-fat diet combined with pharmacological therapy, which was successful in restraining the level of serum triglyceride and total cholesterol to a low to medium range during the follow-ups. The patient was found to be a rare novel homozygous duplication variant—c.45_48dupGCGG (Pro17Alafs*22) in GPIHBP1 gene—leading to a frameshift which failed to form the canonical termination codon TGA. The mutant messenger RNA should presumably produce a peptide consisting of 16 amino acids at the N-terminus, with 21 novel amino acids on the heels of the wild-type protein.Conclusions: Our study expands on the spectrum of GPIHBP1 variants and contributes to a more comprehensive understanding of the genetic diagnosis, genetic counseling, and multimodality therapy of families with severe hyperlipidemia. Our experience gained in this study is also contributory to a deeper insight into severe hyperlipidemia and highlights the importance of molecular genetic tests.
Annual Health Examination Didn’t Perform a Positive Effect in the Prevention of Hyperlipidemia and Hyperglycemia Through an Eightyear Study in China
Authors: zhang xiaohong , he xiaokui , zhou xuehong , geng hongyan , liu xiangyi
Published: October 2021

Background: Annual health examination is recommended for the prevention and treatment of cardiovascular diseases and diabetes. However, whether it is effective for the prevention of hyperlipidemia and hyperglycemia remains unclear. Methods: A retrospective analysis of clinical samples using the laboratory information system was performed. From 2012 to 2019, 5043 participants (1755 males and 3288 females) have completed the annual health examination for 8 consecutive years, which was the follow-up group. In the same period, 136,994 participants (the control group) had a health examination only 1 time. Serum levels of fasting triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) and glucose (GLU) were measured.Results: Compared with 2019 of the control group, serum TC and GLU in the 8th year of the follow-up group increased markedly in some of age groups (p < 0.001). Serum TG and TC in the 8th year were much higher compared with the 1st year of the follow-up group, especially for the female (p < 0.001). Moreover, the prevalence of hyperlipidemia and hyperglycemia in the 8th year showed significant increase compared with the 1st year of the follow-up group and 2019 of the control group (all p < 0.05).Conclusions: Annual health examination didn’t perform a positive effect in the prevention of hyperlipidemia and hyperglycemia. Health management should be paid more attention to prevent cardiovascular disease and diabetes.
High diagnostic performance of a novel reverse transcription loop-mediated isothermal amplification (RT-LAMP) kit for the rapid detection of SARS-CoV-2
Authors: Domnich Alexander , Orsi Andrea , Panatto Donatella , De Pace Vanessa , Ricucci Valentina , Caligiuri Patrizia , Guarona Giulia , Chessa Valerio , Ferone Diego , Boccotti Simona , Bruzzone Bianca , Icardi Giancarlo
Published: October 2021

Although the reverse transcription polymerase chain reaction (RT-PCR) is considered a standard-of-care assay for the laboratory diagnosis of SARS-CoV-2, several limitations of this method have been described. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is an alternative molecular assay and is potentially able to overcome some intrinsic shortcomings of RT-PCR. In this study, we evaluated the diagnostic performance of the novel HG COVID-19 RT-LAMP assay. In this retrospective analysis, a total of 400 routinely collected leftover nasopharyngeal samples with a known RT-PCR result were tested by means of the HG COVID-19 RT-LAMP assay. The overall sensitivity and specificity values of HG COVID-19 RT-LAMP versus RT-PCR were 97.0% (95% CI: 93.6–98.9%) and 98.5% (95% CI: 95.7–99.7%), respectively. Inter-assay agreement was almost perfect (κ = 0.96). Concordance was perfect in samples with high viral loads (cycle threshold <30). The average time to a positive result on RT-LAMP was 17 min. HG COVID-19 RT-LAMP is a reliable molecular diagnostic kit for detecting SARS-CoV-2, and its performance is comparable to that of RT-PCR. Shorter turnaround times and the possibility of performing molecular diagnostics in the point-of-care setting make it a valuable option for facilities without sophisticated laboratory equipment.
Development and Preliminary Testing of a Decision aid to Support Hodgkin Lymphoma Survivors Considering Lung Cancer Screening
Authors: Broadbent Rachel , Seale Tania , Armitage Christopher J. , Linton Kim
Published: October 2021

BackgroundDecisions aids (DA) can support patients to make informed decisions about screening tests. This study describes the development and initial evaluation of a lung cancer screening (LCS) DA targeted towards survivors of Hodgkin lymphoma (HL).MethodsA prototype decision aid booklet was developed and subsequently reviewed by a steering group who provided feedback. Revisions were made to produce the DA tested in this study. HL survivors were recruited to an online survey and/or focus groups. Lymphoma practitioners were invited to an interview study. In the online survey, decisional conflict scales and knowledge scales were completed before and after accessing the DA. The focus groups and interviews explored acceptability and comprehensibility and the decisional needs of stakeholders. Focus groups and interviews were audio recorded. The framework method was used to analyse qualitative data. Results38 HL survivors completed the online survey. Following exposure to the DA, knowledge of LCS and risk factors and decisional conflict scores (total score and subscale scores) improved significantly. 11 HL survivors took part in two focus groups (n=5 and n=6) and 11 practitioners were interviewed. Focus group and interview results: The language, format and length were considered acceptable. Both groups felt the DA was balanced and presented a choice. Icon arrays were felt to aid comprehension of absolute risk values and for some survivors, they reduced affective risk perceptions. Among survivors, the impact of radiation risk on decision making varied according to gender and screening interval, whilst practitioners did not anticipate it to be a major concern for patients. Both groups expressed that a screening offer could mitigate anxiety about lung cancer risk. As anticipated by practitioners, survivors expressed a desire to seek advice from their clinical team. Practitioners thought the DA would meet their informational needs regarding lung cancer screening when supporting survivors. ConclusionsThe DA tested in this study is considered acceptable by HL survivors and their practitioners. The DA reduces decisional conflict and improves knowledge in HL survivors, suggesting that it would support HL survivors to make informed decisions when considering lung cancer screening in a future clinical trial.
Protocol for a scoping review study to identify occupational health hazards among healthcare providers and ancillary staff in Ghana
Authors: Tawiah Philip Apraku , Baffour-Awuah Alberta , Appiah-Brempong Emmanuel , Afriyie-Gyawu Evans
Published: October 2021

Introduction: The formation, modification and implementation of occupational health and safety policy for the Ghana health care industry hinges on data and reviews on occupational exposures. However, there is no synthesized review to speak to the issues of these occupational exposures. A scoping review on occupational exposures among the health workforce in Ghana will provide a broad overview of exposures, and can guide and assist in making decisions on occupational health issues relating healthcare workers. Methods: The Arksey and O’Malley’s scoping review methodology framework will guide the conduct of this scoping review. Primary research studies, government documents and other information on occupational exposures among healthcare workers published in English language will be retrieved from databases, including PubMed, CINAHL, Embase, Medline, Scopus, PsycINFO and Google scholar. Also, grey literature sources in Ghana including government and tertiary institutions websites will be searched. Reference list of key studies will also be screened to identify relevant studies for inclusion. The review will consider studies that address prevalence, knowledge and predisposing factors of occupational exposures along with utilization of occupational hazards preventive measures. After initial removal of duplicates, title and abstract screening, relevant articles will be subjected to full text analysis. The screening processes will be conducted independently by two reviewers. Data will then be extracted and presented in tabular form with a narrative to aid easy comprehension. Ethics and dissemination: This scoping review sought to identify predominant occupational exposures among healthcare providers and ancillary workers as described in published and unpublished literature. In addressing the increase of occupational exposures among healthcare workforce in Ghana, this scoping review is intended to bring all the literature together and predict the areas of occupational exposures that needs attention and redress. The findings of the study will be disseminated through publications, conference presentations and stakeholder meetings.
Analytical Methods for Assessing Retinal Cell Coupling Using Cut-Loading
Authors: Myles William E. , McFadden Sally Anne
Published: October 2021

Electrical coupling between retinal neurons contributes to the functional complexity of visual circuits. “Cut-loading” methods allow simultaneous assessment of cell-coupling between multiple retinal cell-types, but existing analysis impedes direct comparison with gold standard direct dye injection techniques. Therefore cut-loading was used to assess coupling strength in a-type horizontal cells in dark-adapted Guinea pig retinae (n=29) using the standard protocol (Method 1) compared with two non-linear methods. Method 1 describes the distance of dye-diffusion (space constant), while Method 2 extracted the coupling coefficient (kj) and Method 3 measured the diffusion coefficient (De). Dye transfer was measured after one of five diffusion times (1-20 mins), or with a coupling inhibitor, meclofenamic acid (MFA) (50–500µM after 20 mins diffusion). Method 1 includes background fluorescence, producing less accurate coupling estimates than measuring the fluorescence of individual cell-soma (p<0.001). The space constant (Method 1) increased with diffusion time (p<0.01), whereas Methods 2 (p=0.54) and 3 (p=0.63) produced consistent results across all diffusion times. Method 1 was less sensitive to detecting changes induced by MFA than Methods 2 (p<0.01) and 3 (p<0.01). Comparatively, Methods 2 and 3 proved more sensitive and generalisable; allowing for detailed assessment of the coupling between different populations of gap-junction linked cell networks.
The Mirror Reversal Mechanism on Black Hole Collapse and Singularity Eruption in Cosmic Continuum
Authors: Wang Xijia
Published: October 2021

In Cosmic continuum, the cosmic system collapse into a Schwarzschild black hole under the action of a strong gravitational field, and the Planck spheres at the center of the black hole continues to collapse into dark mass bodies, forming dark celestial body and singularity. The Schwarzschild radius is the upper limit of a black hole, and the Planck sphere is the lower limit of a black hole. The singularity is the conversion point between the old and new cosmic systems. The singularity erupts the Planck spheres under the action of a strong gravitational field, and the Planck spheres expands outward to form a new cosmic system. The Planck sphere is both the end of the old cosmic system and the starting point of the new cosmic system. The black hole collapse and the singularity eruption are mirror images of each other. The Planck sphere is the front of the mirror, and the singularity is the back of the mirror.
Hypocalcemia and Massive Pulmonary Embolism in SLE Patient with COVID-19 Infection, A Case Report
Authors: Yousif Mohammed Yousif Elnaeem , Eljack Moh.Mah.Fadel Allah , Alfatih Mohammed , Abdulkarim Mohammed , mohamed Abdirizak Ibrahim , Ahmed Khabab Abbasher Hussien Mohamed , Haroun Mazin Salah-Eldien Hassan
Published: October 2021

A 16-year-old female presented to our ED with fever and coughing of blood for 3 days. She is known to have SLE for 5 months and takes oral prednisone. She was tested positive for COVID19. She developed hypocalcemia with clinically-diagnosed massive pulmonary embolism. She was treated with heparin and recovered.
International overview of business profiles from the perspective of Instagram users
Authors: Machova Renata , Santa Kamilla , Basa Patrik
Published: October 2021

Since creating a business Instagram profile is accessible, an increasing number of businesses discovered a new marketing potential. In addition to large enterprises, many self-entrepreneurs and small enterprises register on the websites. They use Instagram as a marketing tool. The authors noted that many valuable publications have already been published addressing the issue. The main objective of this study is to examine the business profiles in terms of Instagram users. The theoretical background to study was prepared using the available scientific literature in social media and Instagram marketing. The central question of the empirical research is whether it is necessary to perceive business profiles in different terms if the company is operating in multiple countries. The research operated with data from three European countries: Slovakia, Hungary, and Romania. The main goal was to find out how Instagram users in the surveyed countries relate to business profiles. The research questions were as follows: Do Instagram users follow business profiles? How developed is the user’s brand recognition ability? What kind of posts do they like on business profiles? Do they show a willingness to advertise products on their profile? The online questionnaire survey was conducted between February 2019 and December 2020. The obtained data were analyzed in Microsoft Excel and SPSS statistical program. The methodological tools of research were cross-tabulation analysis to analyze the research hypotheses and graphical analysis to present the research results. Answers of Instagram users were analyzed by country. The results detected differences in users’ attitudes towards the business profiles by the countries involved in the research. There are only negligible differences, which do not indicate that treating Instagram users in the studied countries different would be necessary. The research results are accessible for businesses, marketing professionals, and agencies operating in one or all countries involved in the survey or outside the research area.
Development of sharing economy-based business models in the tourism sector
Authors: Navickas Valentinas , Petroke Ieva , Baciuliene Vaida , Kljucnikov Aleksandr
Published: October 2021

The sharing economy is rapidly gaining popularity, especially in the tourism sector. The sharing economy transforms functioning business models, giving businesses new economic activities and development opportunities. However, while sharing economy-based models are spreading in the world, not only the advantages but also the disadvantages are seen. This paper summarizes the arguments within the scientific discussion on the economic characteristics of sharing economy. It identifies the actions determining the development of sharing economy in the tourism sector. Since it is known that the sharing economy is developing rapidly, the development of the sharing economy is usually analyzed as a whole, regardless of the different sectors. However, the development of the sharing economy in the tourism sector is found to be the fastest. Besides, there is the largest number of stakeholders. Systematization of scientific treatises and approaches concerning the investigated issue indicated that the main drivers for the sharing economy development in the tourism sector are the Internet and technology among tourists, the cyclical nature of the economy, the change of societal values, and environmental friendliness. The methodological base of this research was secondary data analysis. The research empirically confirmed and theoretically proved that the sharing economy in the tourism sector develops rapidly, attracts more and more consumers in the tourism sector, and outpaces traditional business models. The obtained results could be used for analyzing the most favorable conditions to develop new business models in the market, analyze the response of market participants (consumers and traditional business model), promote new business initiatives in the tourism sector, research the sharing economy phenomenon in narrower tourism (transport, entertainment, and tourist information platforms).
Marketing in social innovations targeted at healthcare
Authors: Hanulakova Eva , Dano Ferdinand , Kukura Marek , Hula Robert
Published: October 2021

The paper deals with the implementation of marketing in social innovations, types of marketing, and the effects they can bring in the sphere of social innovations. The principalaim of the paper is to explain the possibilities of marketing approach implementation in social innovations and point out some specific areas of marketing which can contribute to more efficient applicability of social innovations and reaching a desirable change with social added value. The authors focused on the sphere of health and the population attitudes to its protection, emphasizing breast carcinoma prevention. The research was implemented on a sample of Slovak women to identify the level of women’s awareness of this issue, whether they are familiar with the methods of protecting their health and whether they use them. This study involved the methods of cluster analysis and binary logistic regression. The research uncovered the facts that are truly alarming from the perspective of societal benefit and women’s health protection. The respondents’ insufficient awareness and low activity in the field of their health protection result in the low level of prevention in this area on the side of women and on the side of medical doctors – specialists, particularly gynecologists. Such a situation includes the women’s insufficient awareness of prevention, low motivation, insufficient accessibility and validity of the needed data and precision, and doctor specialists’ lack of awareness of the possibilities and tools available to improve this area. That is exactly the space allowing for the use of marketing in a whole spectrum of its tools and processes and specific solutions capable of delivering the desired societal change and influencing women’s behavior in the preferred direction. Besides, it is especially effective to implement social marketing and social marketing programs that would mediate necessary information to the receivers and stimulate their motivation towards the desired approach to their health protection. The use of neuromarketing would be beneficial. It would be reflected in the better accuracy of the survey and thus the higher quality of the answers obtained. Based on them, it is subsequently possible to create better-targeted campaigns and strategies of social marketing that would approach the target audience more effectively than in acquiring the information via traditional marketing research methods. The findings would benefit marketing agencies, medical doctors (gynecologists, mammologists), and non-profit organizations actively working in this field.
Estimating the Efficiency of the Green Energy Services’ Marketing Management Based on Segmentation
Authors: Brych Vasyl , Zatonatska Tetiana , Dluhopolskyi Oleksandr , Borysiak Olena , Vakun Oksana
Published: October 2021

Energy security undergoes risks at the level of production, storage, distribution, supply and consumption of energy. Seasonal decrease or increase in demand for energy resources at the consumers’ level due to climate change has a negative impact on the energy system balancing. The article is devoted to estimating the factors of the green energy services’ marketing management and developing a model for estimating of green energy as an innovative product based on the segmentation of the energy market. The energy market segmentation allows to single out its basic components based on the use and consumption of energy from natural non-renewable sources, and the production and consumption of alternative energy (green energy), as well as energy services of different levels. To determine the factors of the green energy services marketing management, the energy market was segmented. The innovative development of green energy as a product, green energy services and smart energy grid based on the use of energy from natural renewable sources was discovered. There is a positive trend of increasing demand for electric vehicles and expanding the geography of charging stations. For further positioning of green energy as an innovative product in the energy market, it is advisable to conduct educational campaigns to promote electric vehicles, study the environmental behavior of transport users et. The active process of the Ukrainian energy market reforming is based on competition and considers the international experience of reform. In particular, the electricity market, the gas market, which ensure the operation of energy distribution systems, the diversification of energy supply sources, and the possibility of independent choice of consumers of gas or electricity suppliers are already functioning. This led to the use of an integrated segment approach to modeling the estimating of green energy as an innovative product in the energy market. To determine the green energy estimating as an innovative product in the energy market, used of the interval models described by the discrete difference equations. Taking into account the above, a promising area for further research is to develop a methodological approach to assessing the management model for the promotion of green energy services in the context of the development of smart grids.
The influence of advertising on children's buying behaviour: a case study in Slovakia
Authors: Hajduova Zuzana , Hutmanova Nikoleta , Jusko Lubor , Molitoris Ludovít
Published: October 2021

Advertising is an essential tool in influencing the customer's buying behavior. However, customers tend to have various levels of understanding and decoding advertising messages. Even though children do not have purchasing power themselves, they still create a key customer segment that can influence the purchase decision of the whole family. The paper focuses on how media usage by children and their attitudes towards advertisements determines their buying behavior. The main purpose of the research is to present and describe how children's advertising literacy develops and its connection with the concept of the theory of mind. The relevance of the decision of this scientific problem is that according to several types of research, children are increasingly targeted through various advertising media. Even though their advertising literacy is not fully developed yet. However, nowadays, there is a growing importance of media in our lives and a need to socialize children as consumers because they represent a huge market for advertisers. An empirical study was carried out by designing a questionnaire filled by Slovak children between the ages of 11 and 15. The advertising impact and the degree of impact of the selected types of advertising media on children's consumer behavior were evaluated. The study involved the online method of computer-assisted web interviewing to investigate the influence of advertising media. The authors assessed the degree of the disruptive effect of the selected advertising media on children and the perception of a chosen medium within gender. The most and the least influential type of media were identified. The findings showed no significant difference within gender in the case of advertising media perception by children. The research empirically confirmed and theoretically proved commercial messages aimed at children through different kinds of traditional and electronic media. According to the results, children's buying behavior is mostly influenced by advertising on social media platforms. This type of media mostly integrates sponsored content which has a huge influence on decision making and forming future preferences. The research results can be helpful for further research of commercial messages of social media aimed at children and how those messages affect the whole family and children's future preferences.
Internal website presentations of Czech luxury fashion businesses in the COVID-19 era
Authors: MacGregor Pelikanova Radka
Published: October 2021

This paper summarizes the arguments and counterarguments within the scientific discussion on the issue of tools boosting marketing, management, and innovations via digital platforms, such as internal websites of the top Czech luxury fashion businesses in the COVID-19 era. The main purpose of the research is to perform a case study about how these websites address COVID-19, innovations, sustainability, and corporate social responsibility. Systematization of the scientific sources and approaches for solving the problem indicated that the COVID-19 pandemic has heavily impacted industries that are dispensable and, at the same time, costly. The relevance of this scientific problem's decision matters theory and practice since the COVID-19 crisis is rather a negative challenge than a positive opportunity for businesses, which often ends up paying the ultimate price – falling into bankruptcy. A battery of employed methodological tools includes a simplified Delphi method with data processing by a panel of experts, the meta-analysis and content analysis, and teleological interpretations instruments. The research object is the Summer 2021 version of the internal websites of the top 20 Czech luxury fashion businesses based on their pre-COVID-19 turnover in 2018. The paper presents an empirical analysis based on this case study and juxtaposes it to prior academic findings. The research empirically confirmed and theoretically proved the underplayed potential of the websites of luxury fashion businesses. The research results could be helpful for theory adjustments and have practical implications and offer recommendations for businesses. They lead to propositions about the internal perspective and digital readiness to inform about it and a general attitude to the marketing, management, and innovations during crises.
Scenario analysis of the expected integral economic effect from an innovative project
Authors: Andros Svitlana , Akimov Oleksandr , Akimova Liudmyla , Chang Shichao , Kumar Gupta Sandeep
Published: October 2021

The purpose of the article is to analyse conceptual approaches to determining the financial feasibility of an innovative project and to develop a methodology for assessing the expected integral economic effect of an innovative project based on a set of performance indicators while ensuring the compatibility of the considered scenarios. Analysed are theoretical approaches to assessing the effectiveness of an innovative project in conditions of uncertainty. The features of evaluating the effectiveness of innovative projects are identified, and the need to assess the expected integral economic effect of socially significant innovative projects is substantiated. The indicators of efficiency and feasibility of evaluating an innovative project in the context of applicability in various economic conditions are critically studied. A set of indicators for evaluating the effectiveness of innovative projects has been determined based on the provisions of the integral approach, and these indicators have been systematised based on their relationship. The expected integral economic effect or possible damage from the implementation of an innovative project has been calculated. The expected integral effect of an innovative project with given intervals of probabilities for individual scenarios is calculated. An integrated methodology for assessing the integrated effectiveness of innovative projects is proposed, which combines quantitative and qualitative performance indicators, economic and non-economic effects. The key directions for the selection of effective innovative solutions in the presence of uncontrollable factors, taking into account a variety of indicators, have been determined. A methodology has been developed for evaluating effective innovative projects with a lack or absence of information about the conditions for their implementation and functioning. Methods have been developed for determining the best options for innovative projects based on the theory of multicriteria choice while ensuring the compatibility of the considered scenarios.
Possibilities of using information resources In bioremediation
Authors: Babynin E. V. , Degtyareva I. A.
Published: October 2021

Abstract: Bioremediation using microorganisms has a number of advantages over physical and chemical methods of water, soil and atmosphere purification. Microorganisms have a wide range of metabolic capabilities that enable them to convert, modify and utilize toxic pollutants for energy and biomass production. This article shows their participation in the decomposition of various industrial wastes, such as dyes, hydrocarbons, chlorinated aromatic compounds and pesticides, among others. Although the use of microorganisms is an environmentally friendly and promising way of solving environmental threats, many factors affect the effectiveness of bioremediation, such as the chemical nature of pollutants, their accessibility to microorganisms, the physical and chemical characteristics of the environment, as well as the interaction of the destructive organisms with each other. The search for new effective strains or the creation of superdestructors using genetic and protein engineering methods proves to be crucial under current circumstances. This task can be solved using such “tools” as genomics, proteomics, transcriptomics and metabolomics. These technologies require the integration of a huge amount of data, which cannot be achieved without the use of bioinformatics. Bioinformatics is used in microbial bioremediation in different ways: analysis of genome sequencing data, identification of protein-coding genes, comparative analysis to identify the function of unknown genes, automatic reconstruction and comparison of metabolic pathways, and study of protein–protein and protein–DNA interactions to understand regulatory mechanisms. This review aims to highlight various resources that store information about possible pathways of microbial metabolism involved in the biodegradation of petroleum products. The use of such information resources can become a starting point for many studies in bioremediation.
Development of a probiotic for animals and aquaculture based on Bacillus toyonensis B-13249 and Bacillus pumilus B-13250 strains
Authors: Malkova A. V. , Evdokimov I. Yu. , Shirmanov M. V. , Irkitova A. N. , Dudnik D. E.
Published: October 2021

Abstract: This article aims to develop a probiotic for animals and aquaculture based on the Bacillus toyonensis B-13249 and Bacillus pumilus B-13250 strains. The selection of a nutrient medium was conducted for cultivating the inoculum of these microorganisms. Several bacteria fermentations of the Bacillus genus were performed in biological reactors with a capacity of 15 and 250 l. A technology for obtaining a finished probiotic for animals and aquaculture was developed. The results indicate that L-broth is the most optimal nutrient medium for cultivating the studied strains. The cultivation of B. toyonensis B-13249 and B. pumilus B-13250 strains in fermenters revealed that sporulation begins after 4–8 hours of fermentation. In contrast to the vegetative medium, the fermentative medium helped the bacilli develop a higher optical density (the maximum value in the B. pumilus strain – 2.400±0.149), pH value (maximum value in the B. toyonensis strain – 8.483±0.609) and titer (at least 1010 CFU/g). After 20–24 hours of incubation, both strains of bacilli in the fermenter, almost completely pass into endospores, which serve as a signal for the start of biomass centrifugation. This was indicated by the following: from a 15 l fermenter – 83.3±6.1 g of concentrate, from a 250 l fermenter – 499.8±51.4 g. The number of bacilli in a concentrated state was at least 1·1011 CFU/g for both strains. Obtaining a finished preparation required mixing bacterial concentrates with maltodextrin to a titer of at least 1·1010 CFU/g. The number of bacteria in the preparation checked every month during the year, recorded no value less than 1·1010 CFU/g. Thus, L-broth is most favorable for growing the mother culture of the B. toyonensis B-13249 and B. pumilus B-13250 strains, and fermentative nutrient medium – for the cultivation in fermenters. The expiry date of the bacilli-based biological preparation is at least 12 months, during which the drug’s polycomponence, color and consistency are preserved, in addition to the bacteria titer (at least 1·1010 CFU/g) and their viability.
Bacterial nanocellulose and softwood pulp for composite paper
Authors: Gismatulina Yu. A. , Budaeva V. V. , Sitnikova A. E. , Bychin N. V. , Gladysheva E. K. , Shavyrkina N. A. , Mironova G. F. , Sevastyanova Yu. V.
Published: October 2021

Abstract: Scaling biosynthesis of bacterial nanocellulose (BNC) allowed samples of composite paper with an increased proportion of BNC to be obtained. This work aims to study BNC samples and bleached soft wood kraft pulp (BSKP) composite paper with a ratio of components varying across a wide range: 10:90, 30:70, 50:50, 60:40, 70:30, 90:10. The method of paper manufacturing was chosen based on the determinations of strength and deformation properties of composite samples with the BNC:BSKP ratio of 20:80. Surface application of BNT on BSKP handsheet provided for an increase in the strength values (tear resistance – by 37%, burst index – by 17%) and deformation characteristics (tension stiffness – by 66%, fracture work – by 8%, breaking length – by 4%) compared to a reference sample. The formation of composites is confirmed in all samples. Scanning electron spectroscopy revealed that paper composites comprise interlaced micro BSKP and nano BNC fibres. As the proportion of BNC in composites elevated, densification of the structure was observed due to an increased fraction of cross-linked nanosized elements. IR spectroscopy indicated the resemblance of cellulose structure in all samples. It was found that an increase in the degree of polymerisation of composite paper is directly proportional to an increase in the BNC amount in the samples. The filtering ability of composite paper samples against microorganisms in the culture liquid of the Medusomyces gisevii Sa-12 producer was studied. It should be noted that yeast retention is achieved with 70% BNC in the paper composite. The presented properties of the new material determine prospects for its use in filtering microorganisms.
Fire safety assessment of the shopping and entertainment center in Samara
Authors: Aleksandrova V. E.
Published: October 2021

Despite all the advantages, shopping centers belong to the facilities with a high risk of emergencies, such as fires. These objects are exposed to natural emergencies, and pose a threat due to possible acts of terrorism and human factors. The purpose of this study is to simulate a fire and develop measures aimed at eliminating it. Modern technological solutions play a crucial role in ensuring the safety of shopping centers. An emergency situation (ES) occurs unexpectedly and develops spontaneously. It is necessary to act quickly and accurately. To carry out rescue and other urgent operations during the elimination of consequences, operational information on the facility and the adjacent territory is required.