bluuebunny/crossref_metadata_2025 huggingface dataset:
Beneath the Top of the Iceberg: Financial Capacity Deficits in Mixed Dementia with and without Depression
Authors: Giannouli Vaitsa , Tsolaki Magdalini
Published: February 2023

Nowadays, controversy exists regarding the influence of comorbid depression on cognition in old age. Additionally, we still know little about the influence of depression in mixed dementia (MD), that is, in cases where there is the co-existence of Alzheimer’s disease and vascular dementia (VaD). Given that the assessment of financial capacity is pivotal for independent living as well as in the prevention of financial exploitation and abuse in old age, in this pilot study, we aimed to examine whether comorbid depression in MD patients can influence financial capacity performance. A total of 115 participants were recruited. They were divided into four groups: MD patients with and without depressive symptoms and healthy elderly without depression as well as older adults suffering from depression. Participants were examined with a number of neuropsychological tests, including the Mini-Mental State Examination (MMSE), Geriatric Depression Scale (GDS-15), and Legal Capacity for Property Law Transactions Assessment Scale (LCPLTAS). The results of this study suggested that financial capacity as measured with LCPLTAS in MD patients was severely impaired when depression co-existed compared to patients suffering only from depression and healthy controls. Deficits in financial capacity in MD and comorbid depression should be a point on which healthcare professionals should focus during neuropsychological assessment in order to prevent financial exploitation.
Mutual Effects of Zinc Concentration and Ratio of Red-Blue Light on Growth and Nutritional Quality of Flowering Chinese Cabbage Sprouts
Authors: Li Xiaopeng , Liu Kaizhe , He Xinyang , Chen Yongkang , Tan Jiehui , Jiang Haozhao , He Rui , Li Yamin , Liu Houcheng
Published: February 2023

The nutritional quality and biomass of various sprouts can be enhanced by Zn and red-blue light, especially the Brassica sprouts. However, the combined effects of this two on sprouts are rarely reported. In this study, different Zn concentrations (0, 1.74, 3.48, 10.43 and 17.39 mM) were combined with two ratios of red-blue light-emitting diodes (LEDs) (R: B = 1:2, 1R2B; R: B = 2:1, 2R1B, at 70 μmol m−2 s−1 PPFD, 14 h/10 h, light/dark) to investigate their mutual effects on the growth, mineral elements, and nutritional quality in flowering Chinese cabbage sprouts (FCCS). Fresh weight, dry weight, contents of organic Zn, soluble sugar, vitamin C, total flavonoids, total polyphenol, FRAP (ferric ion-reducing antioxidant power) and DPPH (radical inhibition percentage of 1,1-diphenyl-2-picrylhydrazyl) were significantly increased by Zn supplement (10.43 and 17.39 mM) and 2R1B, while hypocotyl length and moisture content were decreased remarkably by Zn supplement. Total glucosinolates contents in the sprouts increased dramatically under 2R1B compared with 1R2B, while photosynthetic pigments contents decreased. Heat map and principal component analysis showed that 2R1B + 17.39 mM Zn was the optimal treatment for the accumulation of biomass and health-promoting compound in FCCS, suggesting that a suitable combination of light quality and Zn supplement might be beneficial to zinc-biofortified FCCS production.
HeLoDL: Hedgerow Localization Based on Deep Learning
Authors: Meng Yanmei , Zhai Xulei , Zhang Jinlai , Wei Jin , Zhu Jihong , Zhang Tingting
Published: February 2023

Accurate localization of hedges in 3D space is a key step in automatic pruning. However, due to the irregularity of the hedge shape, the localization accuracy based on traditional algorithms is poor. In this paper, we propose a deep learning approach based on a bird’s-eye view to overcoming this problem, which we call HeLoDL. Specifically, we first project the hedge point cloud top-down as a single image and, then, augment the image with morphological operations and rotation. Finally, we trained a convolutional neural network, HeLoDL, based on transfer learning, to regress the center axis and radius of the hedge. In addition, we propose an evaluation metric OIoU that can respond to the radius error, as well as the circle center error in an integrated way. In our test set, HeLoDL achieved an accuracy of 90.44% within the error tolerance, which greatly exceeds the 61.74% of the state-of-the-art algorithm. The average OIoU of HeLoDL is 92.65%; however, the average OIoU of the best conventional algorithm is 83.69%. Extensive experiments demonstrated that HeLoDL shows considerable accuracy in the 3D spatial localization of irregular models.
Genomic Survey and Expression Analysis of GLKs in Watermelon (Citrullus lanatus)
Authors: Ding Qiangqiang , Jia Li , Jiang Xiangting , Wang Mingxia , Wang Yan , Jiang Haikun , Yu Feifei , Yan Congsheng
Published: February 2023

Golden2-like (GLK) genes positively regulate chloroplast development, increase crop yields, and improve fruit quality. However, there has been no comprehensive identification and characterization of GLKs in watermelon. In this study, a total of 48 ClGLKs were identified in the watermelon genome. Based on phylogenetic analysis, they were divided into five groups. ClGLKs within the same group showed a similar gene structure and conserved motif compositions. Promoter analysis indicated that cis-elements responsive to light were the most abundant, though cis-elements associated with hormones, stress, and developmental regulation were also identified in ClGLKs promoters. Expression analysis indicated significant responses of some ClGLKs to drought and CGMMV stress, suggesting that these genes may participate in responses to biotic and abiotic stresses. Phenotypic analyses revealed enhanced chloroplast development and increased thylakoid density and chlorophyll content in the pericarp of a “dark green” watermelon cultivar. ClGLK8 was identified as the homolog of GLK1-2, the genes that promote chloroplast development and chlorophyll biosynthesis in fruits, and showed significantly increased expression in accordance with chloroplast development and chlorophyll accumulation. Our results provide detailed knowledge of the ClGLKs, which will enhance efforts to further improve the fruit quality of watermelon.
Insights into the Key Genes in Cucumis melo and Cucurbita moschata ToLCNDV Resistance
Authors: Román Belén , Gómez Pedro , Janssen Dirk , Ruiz Leticia
Published: February 2023

Tomato leaf curl New Delhi virus (ToLCNDV, family Geminiviridae, genus Begomovirus) is a whitefly-transmitted virus that causes widespread damage in Cucurbitaceae and Solaneceae crops worldwide. The Spanish strain, ToLCNDV-ES, affects mainly cucurbit crops and has spread through the Mediterranean basin since its first detection in 2013 in the south of Spain. The control of the virus has been based on the adoption of measures to control the vector, which have not been sufficient to reduce production losses. Therefore, the identification of key genes for ToLCNDV resistance is essential for the development of resistant plants. Regarding genetic control of resistance in cucurbit crops, one major locus on chromosome 11 and two additional regions in chromosomes 12 and 2 of C. melo linked to ToLCNDV resistance have been described recently. Concerning C. moschata, a major QTL was also identified on chromosome 8 that resulted in synteny with a QTL on chromosome 11 of C. melo. In this work, we investigated the molecular basis of ToLCNDV resistance in contrasting accessions of C. melo and C. moschata by transcriptional characterization of 10 different candidate genes controlling host factors related to proviral or antiviral mechanisms. Two proviral factor genes, ARP4 in C. melo and SYTA in C. moschata, showed clear differences in expression levels when the susceptible and resistant accessions were compared. The knowledge of proviral factors associated with resistance could be used to screen an active mutagenesis TILLING platform. This is the case of C. pepo, in which no ToLCNDV resistance has been described to date. The relationship between the regulation of the genes ARP4 and SYTA, as well as the genome position of the described loci related to ToLCNDV resistance, is also discussed.
Crowd Density Estimation and Mapping Method Based on Surveillance Video and GIS
Authors: Zhang Xingguo , Sun Yinping , Li Qize , Li Xiaodi , Shi Xinyu
Published: February 2023

Aiming at the problem that the existing crowd counting methods cannot achieve accurate crowd counting and map visualization in a large scene, a crowd density estimation and mapping method based on surveillance video and GIS (CDEM-M) is proposed. Firstly, a crowd semantic segmentation model (CSSM) and a crowd denoising model (CDM) suitable for high-altitude scenarios are constructed by transfer learning. Then, based on the homography matrix between the video and remote sensing image, the crowd areas in the video are projected to the map space. Finally, according to the distance from the crowd target to the camera, the camera inclination, and the area of the crowd polygon in the geographic space, a BP neural network for the crowd density estimation is constructed. The results show the following: (1) The test accuracy of the CSSM was 96.70%, and the classification accuracy of the CDM was 86.29%, which can achieve a high-precision crowd extraction in large scenes. (2) The BP neural network for the crowd density estimation was constructed, with an average error of 1.2 and a mean square error of 4.5. Compared to the density map method, the MAE and RMSE of the CDEM-M are reduced by 89.9 and 85.1, respectively, which is more suitable for a high-altitude camera. (3) The crowd polygons were filled with the corresponding number of points, and the symbol was a human icon. The crowd mapping and visual expression were realized. The CDEM-M can be used for crowd supervision in stations, shopping malls, and sports venues.
Optimization of Surface Preparation and Painting Processes for Railway and Automotive Steel Sheets
Authors: Szalai Szabolcs , Szívós Brigitta Fruzsina , Kurhan Dmytro , Németh Attila , Sysyn Mykola , Fischer Szabolcs
Published: February 2023

The article deals with DIC (Digital Image Correlation) tests on steel plates used in the automotive and railway industries, as well as in the construction industry. The most critical part of DIC tests is the quality of proper surface preparation, painting, and random patterns. The paint mediates the deformation of the optical systems, and its quality is paramount. The authors’ goal in this research is to determine the optimal dye–cleaning–drying time parameters for DIC studies. Commercially available surface preparation and cleaning agents were tested alongside commercially available spray paints. Standard and specific qualification procedures were applied for the measurements. Once the appropriate parameters were determined, the results were validated and qualified by GOM ARAMIS tests. Based on the results, DIC measurements can be performed with higher accuracy and safety in laboratorial and industrial conditions, compared to the traditional deformation measurements executed by dial gauges or linear variable differential transformers.
Optimizing Road Safety Inspections on Rural Roads
Authors: Cantisani Giuseppe , Borrelli Chiara Caterina , Del Serrone Giulia , Peluso Paolo
Published: February 2023

Road safety depends on a complex balance between all the factors that compose the road system: user, vehicle, environment, and infrastructure. Directive 2008/96/EC introduces procedures to improve the European Transport Network (TEN-T) safety, recognizing an important role for safety inspections, but focusing them on freeways and highways. This paper proposed methods and criteria useful to optimize current inspection procedures and adapt them also to secondary and local rural roads. It is often complex to determine the severity and weight of the different risk factors in this context, since deficiencies and faults can be found both in the road infrastructure and in the other components of the system. The proposed survey method was applied to a stretch of the SS7 Appia state road (Lazio, Italy). Producing video capture and using GPS sensors to geolocalize the data proved to be very useful for the compilation of the survey forms. The results showed a good consistency between the safety assessments obtained from the scoring of the inspection forms and the historical accident rate. Therefore, the proposed methodology can be a valuable aid in understanding safety concerns and in defining the improvement actions.
Photobiomodulation with Laser Technology to Reduce Pain Perception during Fixed Orthodontic Treatment: Literature Review and New Perspectives with LED Devices
Authors: Caccianiga Paolo , Carminati Ileana , Caccianiga Gianluigi
Published: February 2023

Despite the growing demand for improving smile aesthetics and occlusal functionality, a significant percentage of patients still refuse or discontinue orthodontic treatment because of pain and discomfort related to this therapy. As consequence, controlling the pain experienced by patients during the same therapy represents a primary concern for both patients and clinicians. Recent pieces of evidence have suggested that photobiomodulation can reduce pain experienced by patients during the decrowding stage or during specific protocols, for example, rapid maxillary expansion. PBM can be performed with lasers and also with a light-emitting diode (LED) device. Nonetheless, few studies on the latter are still present in the literature. The aim of this research is to evaluate the efficacy of photobiomodulation (PBM) with Laser devices in pain management in fixed orthodontic treatment. Only 14 of all articles met the inclusion and exclusion criteria and were therefore used to conduct the research. The different studies compared, in most cases, patients whose mouths were divided into a part treated with PBM and a placebo part. Most of their results show a statistically significant difference in perceived pain between the irradiated arch and the nonirradiated arch. Three authors did not find statistically significant results in favor of PBM, but they used different laser parameters. To obtain generally valid studies, with consistent and reproducible results, it is necessary to standardize the different laser parameters used. LED is less operator-dependent than laser and PBM using this technology seems to have a biological basis similar to that with lasers. In some studies, its clinical efficacy in pain reduction in some orthodontic therapies has been verified. Finally, this article aims to consider LED technology as a future prospect of research on PBM use in orthodontics.
Two Years’ Experience of Intensive Home Hemodialysis with the Physidia S3 System: Results from the RECAP Study
Authors: Fessi Hafedh , Nicoud Philippe , Serrato Tomas , Gilbert Olivia , Courivaud Cécile , Daoud Salima , Morena Marion , Thomas Michel , Canaud Bernard , Cristol Jean-Paul
Published: February 2023

The RECAP study reports results and outcomes (clinical performances, patient acceptance, cardiac outcomes, and technical survival) achieved with the S3 system used as an intensive home hemodialysis (HHD) platform over a three-year French multicenter study. Ninety-four dialysis patients issued from ten dialysis centers and treated more than 6 months (mean follow-up: 24 months) with S3 were included. A two-hour treatment time was maintained in 2/3 of patients to deliver 25 L of dialysis fluid, while 1/3 required up to 3 h to achieve 30 L. The additional convection volume produced by means of the SeCoHD tool (internal filtration backfiltration) was 3 L/session, and the net ultrafiltration produced to achieve dry weight was 1.4 L/session. On a weekly basis, an average 156 L of dialysate corresponding to 94 L of urea clearance when considering 85% dialysate saturation under low flow conditions was delivered. Such urea clearance was equivalent to 9.2 [8.0–13.0] mL/min weekly urea clearance and a standardized Kt/V of 2.5 [1.1–4.5]. The predialysis concentration of selected uremic markers remained remarkably stable over time. Fluid volume status and blood pressure were adequately controlled by means of a relatively low ultrafiltration rate (7.9 mL/h/kg). Technical survival on S3 was 72% and 58% at 1 and 2 years, respectively. The S3 system was easily handled and kept by patients at home, as indicated by technical survival. Patient perception was improved, while treatment burden was reduced. Cardiac features (assessed in a subset of patients) tended to improve over time. Intensive hemodialysis relying on the S3 system offers a very appealing option for home treatment with quite satisfactory results, as shown in the RECAP study throughout a two-year follow-up time, and offers the best bridging solution to kidney transplantation.
Ultra-Wide-Field Fluorescein Angiography Assessment of Non-Perfusion in Patients with Diabetic Retinopathy Treated with Anti-Vascular Endothelial Growth Factor Therapy
Authors: Morel Jean-Baptiste , Fajnkuchen Franck , Amari Fatima , Sritharan Nanthara , Bloch-Queyrat Coralie , Giocanti-Aurégan Audrey
Published: February 2023

Purpose: To follow the evolution of peripheral ischemia by fluorescein angiography (FA) on ultra-wide-field (UWF) images in diabetic patients treated with anti-vascular endothelial growth factor (anti-VEGF) for macular edema. Methods: Prospective, non-interventional cohort study analyzing UWF-FA images of 48 patients with diabetic retinopathy (48 eyes) treated for diabetic macular edema. UWF-FA was performed at baseline and after one year of anti-VEGF therapy (M12). The primary endpoint was the change in the non-perfusion index. Results: Of the 48 patients included in this study, 25 completed the one-year follow-up, and 20 had FA images of sufficient quality to be interpreted. The non-perfusion index did not significantly change from baseline after one year of anti-VEGF treatment (0.7% of the non-perfused area at baseline versus 0.5% at M12; p = 0.29). In contrast, the diabetic retinopathy severity score improved significantly between baseline and M12. Conclusions: Anti-VEGF treatment with aflibercept for diabetic macular edema had no impact on the retinal perfusion assessed by FA, but it allowed for artificially improving diabetic retinopathy severity scores.
Mild and Moderate Hemophilia A: Neglected Conditions, Still with Unmet Needs
Authors: Castaman Giancarlo , Peyvandi Flora , De Cristofaro Raimondo , Pollio Berardino , Di Minno Dario M. N.
Published: February 2023

Hemophilia A (HA) is an inherited X-linked bleeding disorder, caused by the deficiency of coagulation factor VIII (FVIII), with variable clinical phenotypes [...]
Transcatheter Tricuspid Valve Replacement: Illustrative Case Reports and Review of State-of-Art
Authors: Barreiro-Pérez Manuel , González-Ferreiro Rocío , Caneiro-Queija Berenice , Tavares-Silva Marta , Puga Luis , Parada-Barcia Jose , Rodriguez-Perez Alvaro , Baz-Alonso Jose , Pinon-Esteban Miguel , Estevez-Loureiro Rodrigo , Iniguez-Romo Andres
Published: February 2023

Tricuspid regurgitation (TR) is one of the most common heart valve diseases, associated a with poor prognosis since significant TR is associated with an increased mortality risk compared to no TR or mild regurgitation. Surgery is the standard treatment for TR, although it is associated with high morbidity, mortality, and prolonged hospitalization, particularly in tricuspid reoperation after left-sided surgery. Thus, several innovative percutaneous transcatheter approaches for repair and replacement of the tricuspid valve have gathered significant momentum and have undergone extensive clinical development in recent years, with favorable clinical outcomes in terms of mortality and rehospitalization during the first year of follow-up. We present three clinical cases of transcatheter tricuspid valve replacement in an orthotopic position with two different innovative systems along with a review of the state-of-the-art of this emergent topic.
How Radiomics Can Improve Breast Cancer Diagnosis and Treatment
Authors: Pesapane Filippo , De Marco Paolo , Rapino Anna , Lombardo Eleonora , Nicosia Luca , Tantrige Priyan , Rotili Anna , Bozzini Anna Carla , Penco Silvia , Dominelli Valeria , Trentin Chiara , Ferrari Federica , Farina Mariagiorgia , Meneghetti Lorenza , Latronico Antuono , Abbate Francesca , Origgi Daniela , Carrafiello Gianpaolo , Cassano Enrico
Published: February 2023

Recent technological advances in the field of artificial intelligence hold promise in addressing medical challenges in breast cancer care, such as early diagnosis, cancer subtype determination and molecular profiling, prediction of lymph node metastases, and prognostication of treatment response and probability of recurrence. Radiomics is a quantitative approach to medical imaging, which aims to enhance the existing data available to clinicians by means of advanced mathematical analysis using artificial intelligence. Various published studies from different fields in imaging have highlighted the potential of radiomics to enhance clinical decision making. In this review, we describe the evolution of AI in breast imaging and its frontiers, focusing on handcrafted and deep learning radiomics. We present a typical workflow of a radiomics analysis and a practical “how-to” guide. Finally, we summarize the methodology and implementation of radiomics in breast cancer, based on the most recent scientific literature to help researchers and clinicians gain fundamental knowledge of this emerging technology. Alongside this, we discuss the current limitations of radiomics and challenges of integration into clinical practice with conceptual consistency, data curation, technical reproducibility, adequate accuracy, and clinical translation. The incorporation of radiomics with clinical, histopathological, and genomic information will enable physicians to move forward to a higher level of personalized management of patients with breast cancer.
Is the Surgical Drainage Mandatory for Leak after Sleeve Gastrectomy?
Authors: Nedelcu Marius , Manos Thierry , Noel Patrick , Danan Marc , Zulian Viola , Vilallonga Ramon , Nedelcu Anamaria , Carandina Sergio
Published: February 2023

Introduction: Despite the unanimous acknowledgement of the laparoscopic sleeve gastrectomy (LSG) worldwide, the leak remains its deficiency. For the last decade, the surgical treatment was practically considered mandatory for almost any collection following LSG. The aim of this study is to evaluate the need for surgical drainage for leak following LSG. Methods: All consecutive patients having gone through LSG from January 2017 to December 2020 were enrolled in our study. Once the demographic data and the leak history were registered, we analyzed the outcome of the surgical or endoscopic drainage, the characteristics of the endoscopic treatment, and the evolution to complete healing. Results: A total of 1249 patients underwent LSG and the leak occurred in 11 cases (0.9%). There were 10 women with a mean age of 47.8 years (27–63). The surgical drainage was performed for three patients and the rest of the eight patients underwent primary endoscopic treatment. The endoscopic treatment was represented with pigtails for seven cases and septotomy with balloon dilation for four cases. In two out of these four cases, the septotomy was anticipated by the use of a nasocavitary drain for 2 weeks. The average number of endoscopic procedures was 3.2 (range 2–6). The leaks achieved complete healing after an average duration of 4.8 months (range 1–9 months). No mortality was recorded for a leak. Conclusions: The treatment of the gastric leak must be tailored to each patient. Although there is still no consensus for the endoscopic drainage of leaks after LSG, the surgical approach can be avoided in up to 72%. The benefits of pigtails and nasocavitary drains followed by endoscopic septotomy are undeniable, and they should be included in the armamentarium of any bariatric center.
Entomopathogenic Fungi as a Potential Management Tool for the Control of Urban Malaria Vector, Anopheles stephensi (Diptera: Culicidae)
Authors: Renuka Siddaramegowda , Vani H Chalageri , Alex Eapen
Published: February 2023

Anopheles stephensi (Diptera: Culicidae) is the vector of urban malaria in India and has a significant impact in transmitting infection in cities and towns. Further, WHO has also alarmed its invasive nature as a threat to African countries. Entomopathogenic fungi such as Beauveria bassiana and Metarhizium anisopliae have been found to be highly effective in controlling vector mosquito populations and therefore could be used in integrated vector control programs. Before employing the entomopathogenic fungi into the control programs, an effective isolate must be selected. Two separate experiments were conducted to evaluate the efficacy of Beauveria bassiana (Bb5a and Bb-NBAIR) and Metarhizium anisopliae (Ma4 and Ma-NBAIR) isolates against An. stephensi. Cement and mud panels were treated with fungal conidia with the concentration of 1 × 107 conidia/mL and adult An. stephensi mosquitoes were exposed to the treated panels (24 h after conidia were applied) by conducting WHO cone bioassay tests. The survival of the mosquitoes was monitored daily until the 10th day. In the second experiment, second instar larvae of An. stephensi were treated with fungal (Bb5a, Bb-NBAIR, Ma4 and Ma-NBAIR) conidia and blastospores with the spore concentration of 1 × 107 spores/mL. The survival of larvae was monitored until pupation. All the fungal isolates tested caused mortality in the adult mosquitoes, with varying median survival times. The Bb5a isolate reported lesser median survival times on both cement and mud panels (6 days). The treated mosquitoes showed similar survival rates for each fungal isolate irrespective of the panel type. There was no mortality in the treated larvae; however, a delay in larval development to pupae was observed compared with the untreated control larvae. Ma4-treated larvae took 11 days (95% CI = 10.7–11.2) to become pupae when compared with the untreated control larvae (6 days [95% CI = 5.6–6.3]). The findings of this study will be useful to consider EPF as a tool for the management of vector mosquitoes.
Sporotrichosis Outbreak Due to Sporothrix brasiliensis in Domestic Cats in Magallanes, Chile: A One-Health-Approach Study
Authors: Thomson Pamela , González Carlos , Blank Olivia , Ramírez Valentina , Río Camila del , Santibáñez Sebastián , Pena Pamela
Published: February 2023

Sporotrichosis is an implantation mycosis with subcutaneo-lymphatic or, more rarely, a viscerally disseminated affection; it can be acquired through traumatic percutaneous inoculation of the fungus present in soil or plant matter, or by feline scratching. Among the causative agents, Sporothrix brasiliensis is considered the most virulent species with a high prevalence in Brazil and recently in Argentina. Objective: To describe a S. brasiliensis outbreak in domestic and feral cats detected in the Magallanes region of southern Chile. Materials and Methods: Between the months of July and September 2022, three cats presented with suppurative subcutaneous lesions located mainly on the head and thoracic limbs. The cytology revealed the presence of yeasts with morphological characteristics suggestive of Sporothrix spp. The histopathology confirmed pyogranulomatous subcutaneous lesions associated with the presence of the same yeasts. The fungal culture followed by the partial gene sequence and analysis of the ITS region confirmed the diagnosis of the S. brasiliensis as the causative agent. The cats were treated with itraconazole associated in one case with potassium iodide. The evolution of the patients was favorable in all cases. Conclusions: An outbreak caused by S. brasiliensis was detected in domestic and feral cats in austral Chile. The correct identification of this fungus and antifungigram is essential for treatment decisions and for designing dissemination control and prevention programs under a one health approach that consider the health of people, animals, and the environment.
The Distinction between Dematiaceous Molds and Non-Dematiaceous Fungi in Clinical and Spiked Samples Treated with Hydrogen Peroxide Using Direct Fluorescence Microscopy
Authors: Juravel Elchanan , Polacheck Itzhack , Isaacson Batya , Dagan Arie , Korem Maya
Published: February 2023

Dematiaceous fungi are pigmented molds with a high content of melanin in their cell walls that can cause fatal infections in immunocompromised hosts. Direct microscopy is the main method for the rapid diagnosis of dematiaceous fungi in clinical specimens. However, it is often difficult to distinguish their hyphae from non-dematiaceous hyphae and yeast pseudohyphae. Our aim was to develop a fluorescence staining method that targets melanin for the detection of dematiaceous molds in clinical specimens. Glass slide smears of clinical samples and sterile bronchoalveolar lavage spiked with dematiaceous and non-dematiaceous fungi were treated with hydrogen peroxide, and digital images were recorded using direct microscopy with different fluorescent filters. The images of fungi were compared for their fluorescence intensity using the NIS-Elements software. The fluorescent signal between dematiaceous and non-dematiaceous fungi demonstrated a markedly increased mean intensity for dematiaceous molds following hydrogen peroxide treatment (7510.3 ± 10,427.6 vs. 0.3 ± 3.1, respectively, p < 0.0001). No fluorescent signal was detected in the absence of hydrogen peroxide. “Staining” fungal clinical specimens with hydrogen peroxide, followed by fluorescence microscopy examination, can differentiate between dematiaceous and non-dematiaceous fungi. This finding can be used for the detection of dematiaceous molds in clinical specimens and enables the early and appropriate treatment of infections.
Liquid Biopsy for Oral Cancer Diagnosis: Recent Advances and Challenges
Authors: Naito Yutaka , Honda Kazufumi
Published: February 2023

“Liquid biopsy” is an efficient diagnostic tool used to analyse biomaterials in human body fluids, such as blood, saliva, breast milk, and urine. Various biomaterials derived from a tumour and its microenvironment are released into such body fluids and contain important information for cancer diagnosis. Biomaterial detection can provide “real-time” information about individual tumours, is non-invasive, and is more repeatable than conventional histological analysis. Therefore, over the past two decades, liquid biopsy has been considered an attractive diagnostic tool for malignant tumours. Although biomarkers for oral cancer have not yet been adopted in clinical practice, many molecular candidates have been investigated for liquid biopsies in oral cancer diagnosis, such as the proteome, metabolome, microRNAome, extracellular vesicles, cell-free DNAs, and circulating tumour cells. This review will present recent advances and challenges in liquid biopsy for oral cancer diagnosis.
Ecological Restoration and Protection of National Land Space in Coal Resource-Based Cities from the Perspective of Ecological Security Pattern: A Case Study in Huaibei City, China
Authors: Li Zixuan , Chang Jiang , Li Cheng , Gu Sihao
Published: February 2023

Mining activities have contributed to the growth of the city, but also raised non-negligible eco-geological environmental issues that threaten ecological safety. Ecological security pattern (ESP), as an important grip on the ecological restoration and protection of national land space, helps to balance mining activities and ecological protection in coal resource-based cities. Taking Huaibei City as a study area, we applied the ESP research paradigm: an ecosystem “function-structure” conceptual framework was developed to identify ecological sources, the “coal mining subsidence—economic activities” framework was used to revise ecological resistance surface, and the circuit theory was used to extract ecological corridors. Then, key areas for ecological restoration and protection were identified, including ecological pinch points, barrier points, and fracture points. Finally, the pattern and strategies for ecological restoration and protection were proposed. Study results show that there were 51 ecological sources, covering an area of 152.75 km2; 111 ecological corridors were extracted with 6000 as truncation threshold; 17 pinch points, 75 barrier points, and 117 fracture points were identified. Ecological restoration and protection patterns of “one axis, two shields, four zones, eight belts and multiple corridors”, and strategies for key areas were proposed. The results of the study are important for the sustainable development of coal-resource-based cities.
Recycling a Mixed Language: Posha in Turkey
Authors: Uzum Melike , Demir Nurettin , Bagriacik Metin
Published: February 2023

We provide a sketch grammar of a new bilingual mixed language based on data gathered through interaction with its last native speakers. The language, which we call Posha of Çankırı, is spoken in central Turkey. The source languages are Turkish and Lomavren, another bilingual mixed language for which the source languages are Armenian and some Central Indo-Aryan varieties. In Posha of Çankırı, the mixing happens in the nominal morphology and in the lexicon while the verbal roots and verbal morphology are entirely from the ancestral language, Lomavren, albeit with certain minor changes. The Indo-Aryan layer of vocabulary is rather thin and the Indo-Aryan retentions in grammar can only be speculated. We show that the emergence of Posha of Çankırı has been initiated by language shift, but that its ultimate defining characteristic is L2 insertions into (some distorted version of) the L1. The study contributes to the documentation of lesser known new varieties and touches upon topics such as the mechanism involved in the emergence of bilingual mixed languages.
Laboratory- and Pilot-Scale Cultivation of Tetraselmis striata to Produce Valuable Metabolic Compounds
Authors: Patrinou Vasiliki , Patsialou Stefania , Daskalaki Alexandra , Economou Christina N. , Aggelis George , Vayenas Dimitris V. , Tekerlekopoulou Athanasia G.
Published: February 2023

Marine microalgae are considered an important feedstock of multiple valuable metabolic compounds of high biotechnological potential. In this work, the marine microalga Tetraselmis striata was cultivated in different scaled photobioreactors (PBRs). Initially, experiments were performed using two different growth substrates (a modified F/2 and the commercial fertilizer Nutri-Leaf (30% TN—10% P—10% K)) to identify the most efficient and low-cost growth medium. These experiments took place in 4 L glass aquariums at the laboratory scale and in a 9 L vertical tubular pilot column. Enhanced biomass productivities (up to 83.2 mg L−1 d−1) and improved biomass composition (up to 41.8% d.w. proteins, 18.7% d.w. carbohydrates, 25.7% d.w. lipids and 4.2% d.w. total chlorophylls) were found when the fertilizer was used. Pilot-scale experiments were then performed using Nutri-Leaf as a growth medium in different PBRs: (a) a paddle wheel, open, raceway pond of 40 L, and (b) a disposable polyethylene (plastic) bag of 280 L working volume. Biomass growth and composition were also monitored at the pilot scale, showing that high-quality biomass can be produced, with important lipids (up to 27.6% d.w.), protein (up to 45.3% d.w.), carbohydrate (up to 15.5% d.w.) and pigment contents (up to 4.2% d.w. total chlorophylls), and high percentages of eicosapentaenoic acid (EPA). The research revealed that the strain successfully escalated in larger volumes and the biochemical composition of its biomass presents high commercial interest and could potentially be used as a feed ingredient.
Hand–Eye Calibration Using a Tablet Computer
Authors: Sato Junya
Published: February 2023

Many approaches have been developed to solve the hand–eye calibration problem. The traditional approach involves a precise mathematical model, which has advantages and disadvantages. For example, mathematical representations can provide numerical and quantitative results to users and researchers. Thus, it is possible to explain and understand the calibration results. However, information about the end-effector, such as the position attached to the robot and its dimensions, is not considered in the calibration process. If there is no CAD model, additional calibration is required for accurate manipulation, especially for a handmade end-effector. A neural network-based method is used as the solution to this problem. By training a neural network model using data created via the attached end-effector, additional calibration can be avoided. Moreover, it is not necessary to develop a precise and complex mathematical model. However, it is difficult to provide quantitative information because a neural network is a black box. Hence, a method with both advantages is proposed in this study. A mathematical model was developed and optimized using the data created by the attached end-effector. To acquire accurate data and evaluate the calibration results, a tablet computer was utilized. The established method achieved a mean positioning error of 1.0 mm.
Relationship between Inflammation and Vasospastic Angina
Authors: Hung Ming-Yow , Hung Ming-Jui
Published: February 2023

Coronary artery spasm (CAS) is a dynamic coronary stenosis causing vasospastic angina (VSA). However, VSA is a potentially lethal medical condition with multiple presentations, including sudden cardiac death. Despite investigations to explore its pathogenesis, no single mechanism has been found to explain the entire process of VSA occurrence. The roles of elevated local and systemic inflammation have been increasingly recognized in VSA. Treatment strategies to decrease local and systemic inflammation deserve further investigation.
Effect of TiO2 and Al2O3 Addition on the Performance of Chitosan/Phosphotungstic Composite Membranes for Direct Methanol Fuel Cells
Authors: Zaffora Andrea , Giordano Elena , Volanti Valentina Maria , Iannucci Leonardo , Grassini Sabrina , Gatto Irene , Santamaria Monica
Published: February 2023

Composite chitosan/phosphotungstic acid (CS/PTA) with the addition of TiO2 and Al2O3 particles were synthesized to be used as proton exchange membranes in direct methanol fuel cells (DMFCs). The influence of fillers was assessed through X-ray diffraction, scanning electron microscopy, thermogravimetric analysis, liquid uptake, ion exchange capacity and methanol permeability measurements. The addition of TiO2 particles into proton exchange membranes led to an increase in crystallinity and a decrease in liquid uptake and methanol permeability with respect to pristine CS/PTA membranes, whilst the effect of the introduction of Al2O3 particles on the characteristics of membranes is almost the opposite. Membranes were successfully tested as proton conductors in a single module DMFC of 1 cm2 as active area, operating at 50 °C fed with 2 M methanol aqueous solution at the anode and oxygen at the cathode. Highest performance was reached by using a membrane with TiO2 (5 wt.%) particles, i.e., a power density of 40 mW cm−2, almost doubling the performance reached by using pristine CS/PTA membrane (i.e., 24 mW cm−2).
Influence of Mg Content on the Corrosion Behavior and Precipitation of Aged Al-Cu-Li-xMg Alloys
Authors: Wu Rong , Liu Zhenzhen , Ning Hong , Lu Dingding , Liu Zhihao , Meng Ya , Li Jinfeng , Zhang Ruifeng
Published: February 2023

Aluminum (Al)–copper (Cu)–lithium (Li) alloys are susceptible to environmental degradation, which limits the in-service lifetime of the components. Because of their complex composition, there are seldom studies focusing on corrosion behavior influenced by one single element, which is essential to clarify the corrosion mechanism. Herein, the sole influence of Mg on the corrosion behavior of Al–Cu–Li–xMg alloys was analyzed. Results revealed that the addition of Mg can affect the nucleation and precipitation process of the T1 precipitate, resulting in a difference of potential between grain interior and grain boundary. The precipitation of the T1 precipitate was promoted in a 0.7Mg alloy while impeded in a 1.1Mg alloy, due to the competition of Cu atoms and nucleation sites between T1 and S′. Two types of corrosion behavior of Al–Cu–Li–xMg alloys appeared due to the potential difference and continuity of grain boundaries. Continuous precipitates in grain boundaries and less precipitates within the grain are likely to cause intergranular corrosion (IGC), while more precipitates within the grain will result in pitting.
Adaptive Expertise in Undergraduate Pharmacy Education
Authors: Steenhof Naomi
Published: February 2023

Pharmacy educators are grappling with concerns around curriculum overload and core pharmacist competencies in a rapidly changing and increasingly complex healthcare landscape. Adaptive expertise provides a conceptual framework to guide educators as they design instructional activities that can support students on their journey towards becoming pharmacists who can perform procedural tasks efficiently, as well as creatively handle new and difficult-to-anticipate problems that arise regularly in pharmacy practice. This article explores undergraduate pharmacy education through a cognitive psychology lens and foregrounds three instructional design strategies which support the development of adaptive expertise: (1) cognitive integration, (2) productive failure, and (3) inventing with contrasting cases. These three evidence-based strategies cultivate long-term learning and provide a practical mechanism to combat curriculum overload and backwards-facing assessments. Pharmacy education can encourage the development of procedural and conceptual knowledge and position pharmacy students to excel as they move into more complicated and ambiguous roles in our healthcare system.
Transmission Enhancement through Sub-Wavelength Aperture Based on Regulable Water-Based Metamaterial
Authors: Shi Pengfei , Li Xiaodong , Zhao Hongge , Tang Youfu , Zhu Jingwei , Gao Renjing , Liu Shutian
Published: February 2023

A realization form of the Mie resonance with adjustable characteristics based on a water-based metamaterial is proposed. A novel local electromagnetic field coupling mechanism based on a water-based metamaterial is established, which is used for transmission enhancement through the sub-wavelength aperture for the first time. The water-based metamaterial particle for transmission enhancement at a specific frequency is designed. By designing the microfluidic control system to control the water filling (i.e., height) of the metamaterial particle, the adjustable transmission enhancement characteristics are realized. The influence of bilateral symmetry and flexible deformation of the metamaterial particle on the transmission characteristics is analyzed. The influence of the design parameters on the enhanced transmission characteristics is discussed.
Exploring the Effect of Optical Beams on Hybrid VLC/RF Transmission Characteristics
Authors: Ding Jupeng , I Chih-Lin , Zhao Kai , Liu Shuxian
Published: February 2023

The hybrid visible light communication/radio frequency (VLC/RF) transmission system could offer consistent coverage and high throughput, which are inherited from the VLC and RF link components separately. However, almost all current hybrid VLC/RF schemes are limited in scenarios adopting light emitting diodes (LED) sources with basic Lambertian optical beams, and fail to investigate the scenarios using sources with distinct non-Lambertian optical beams. For exploring this effect of optical beams on hybrid VLC/RF transmission performance, typical symmetric and non-symmetric optical beams are introduced to constitute representative non-Lambertian VLC/RF links. Moreover, the heterogeneous optical beams-based hybrid VLC/RF transmission scheme is proposed and estimated under a hard switching mechanism. Numerical results show that, compared with an approximate 234.39 Mbps maximum throughput of the conventional hybrid Lambertian VLC/RF configuration, the counterparts of the hybrid non-Lambertian VLC/RF configuration with the Rebel and NSPW optical beams are 203.39 Mbps and 279.34 Mbps, respectively. Furthermore, the proposed heterogeneous beams-based hybrid scheme is applied via Lambertian and non-Lambertian candidate beams, with the average throughput increased to 186.45 Mbps from the original approximate 174.14 Mbps of the conventional Lambertian hybrid VLC/RF configuration.
Histochemical Analysis and Ultrastructure of Trichomes and Laticifers of Croton gratissimus Burch. var. gratissimus (Euphorbiaceae)
Authors: Naidoo Danesha , Naidoo Yougasphree , Naidoo Gonasageran , Kianersi Farzad , Dewir Yaser Hassan
Published: February 2023

Croton gratissimus (Lavender croton) possesses three distinct secretory structures. These include lepidote and glandular trichomes and non-articulated unbranched laticifers. The lepidote trichomes form a dense indumentum on the abaxial surface of the leaves and canopy the glandular trichomes. Although assumed to be non-glandular, transmission electron microscopy (TEM) indicated high metabolic activity within the stalk and radial cells. Glandular trichomes are embedded in the epidermal layer and consist of a single cell which forms a prominent stalk and dilated head. Laticifers occur on the mid-vein of leaves and are predominantly associated with vascular tissue. In the stems, laticifers are associated with the phloem and pith. Both trichome types and laticifers stained positive for alkaloids, phenolic compounds, and lipids. Positive staining for these compounds in lepidote trichomes suggests their involvement in the production and accumulation of secondary metabolites. These metabolites could provide chemical defense for the plant and potentially be useful for traditional medicine.
What Can Boron Deficiency Symptoms Tell Us about Its Function and Regulation?
Authors: Bolaños Luis , Abreu Isidro , Bonilla Ildefonso , Camacho-Cristóbal Juan J. , Reguera María
Published: February 2023

On the eve of the 100th anniversary of Dr. Warington’s discovery of boron (B) as a nutrient essential for higher plants, “boronists” have struggled to demonstrate a role beyond its structural function in cell walls dimerizing pectin molecules of rhamnogalacturonan II (RGII). In this regard, B deficiency has been associated with a plethora of symptoms in plants that include macroscopic symptoms like growth arrest and cell death and biochemical or molecular symptoms that include changes in cell wall pore size, apoplast acidification, or a steep ROS production that leads to an oxidative burst. Aiming to shed light on B functions in plant biology, we proposed here a unifying model integrating the current knowledge about B function(s) in plants to explain why B deficiency can cause such remarkable effects on plant growth and development, impacting crop productivity. In addition, based on recent experimental evidence that suggests the existence of different B ligands other than RGII in plant cells, namely glycolipids, and glycoproteins, we proposed an experimental pipeline to identify putative missing ligands and to determine how they would integrate into the above-mentioned model.