bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluating the Nutrient Contents and Nutritive Value of Taif’s Rose (Rosa damascena Mill var. trigintipetala) Waste to Be Used as Animal Forage or Soil Organic Fertilizers
Authors: M. Galal Tarek , Ali Esmat F. , Eid Ebrahem M. , Al-Yasi Hatim M. , Magrashi Ali , Althobaiti Fayez , Farahat Emad A.
Published: September 2022

This study aimed to evaluate the nutrient content and nutritive value of pruning waste of the Taif Damask rose for its potential use as an organic fertilizer or animal forage in the Taif region, Saudi Arabia. For this purpose, the pruning waste of Taif’s rose and soil samples supporting the plant growth at different ages were collected from four farms (F1: 4, F2: 10, F3: 12, and F4: 20 years old). The total aboveground biomass (AGB) of the plants, stems, and leaves were measured in addition to the stem height and crown diameter. The results showed that the maximum stem height and crown diameter (184.20 and 243.5 cm) were recorded in the oldest farm (F4). Moreover, the stem, leaves, and aboveground biomass (AGB) of the waste were maximal (3.91, 1.30 and 5.21 t ha−1) at F4. F1 had the highest N content (154.30 mg kg−1) in the plant leaves, while F2 had the highest stem N and P (172.33 and P 9.40 mg kg−1). Moreover, F3 had the highest concentrations of leaf P (7.17 mg kg−1), leaf and stem K (112.47 and 277.30 mg kg−1), stem Ca2+ (251.93 mg kg−1), and leaf and stem Mg2+ (122.27 and 123.57 mg kg−1). The stems had higher percentages of total proteins, fibers, ash, and NFE (total carbohydrates) than the leaves in F1 and F2, while the opposite was observed in F3 and F4. The leaves of F2 rose plants had the highest percentage of neutral detergent fibers (NDF), and their stems had the highest percentages of total proteins (10.71%). The leaves of F3 plants had the highest percentage of acid detergent lignin (ADL) and the lowest crude fibers (7.63 and 13.27%), while the stems had the highest NFE (72.71%). The plant–soil relationship expressed by the CCA biplot showed that all the measured plant parameters were at higher positions on the Mg axis, except for the plant height and crown diameter, which were at low positions on the N and NO3 axes, respectively. In contrast, Cl−, NO3−, HCO3−, and SO42− had high positive correlations with axis 1 and negative values with axis 2, while EC, the total P, and Ca2+ had high positive correlations with, and pH had high negative values in relation to, axis 2. Due to its considerable high inorganic and organic nutrient contents, Taif’s rose could be used in the manufacturing of organic fertilizer. Additionally, the analysis of the nutritive value of the pruning waste supports its use as animal forage. We strongly recommend that further studies be conducted on the application of plant waste as a soil amendment and animal forage in the field.
Cover Crops Increase N and P Cycling and Rice Productivity in Temperate Cropping Systems
Authors: Lizcano Toledo Rodolfo , Lerda Cristina , Moretti Barbara , Miniotti Eleonora , Santoro Veronica , Fernandez-Ondoño Emilia , Martin Maria , Said-Pullicino Daniel , Romani Marco , Celi Luisella
Published: September 2022

Cover crops can determine positive benefits on soil fertility and rice productivity, although scant attention has been devoted to evaluating the effects of hairy vetch (Vicia villosa Roth) and the incorporation of rice straw with different N fertilization levels on soil N and P availability and crop yields in temperate cropping systems characterized by poorly developed soils. In this study, the effects of cover crops grown before rice in a temperate mono-cropping system (NW Italy) on: (i) crop yields and yield components; (ii) apparent N fertilizer recovery and optimal level of N fertilization with hairy vetch; and (iii) temporal variation of soil available N and P forms during the hairy vetch growth and rice cropping season, have been investigated. The cultivation and incorporation of hairy vetch in the rice cropping system increased grain productivity by 12%, while reducing N mineral fertilization requirements by 33%. Combined with the incorporation of crop residues, hairy vetch provided a N and P input of 178 and 18 kg ha−1, respectively, representing a readily available source for plant uptake over the whole rice cropping season, particularly under anaerobic conditions. This results in a better temporal synchronization of soil N and P availability with crop nutrient demand, leading to a better rice grain productivity and quality performance.
A Threshold Logistic Modelling Approach for Identifying Thresholds between Antibiotic Use and Methicillin-Resistant Staphylococcus aureus Incidence Rates in Hospitals
Authors: Aldeyab Mamoon A. , Bond Stuart E. , Conway Barbara R. , Lee-Milner Jade , Sarma Jayanta B. , Lattyak William J.
Published: September 2022

The aim of this study was to demonstrate the utility of threshold logistic modelling, an innovative approach in identifying thresholds and risk scores in the context of population antibiotic use associated with methicillin-resistant Staphylococcus aureus (MRSA) incidence rates in hospitals. The study also aimed to assess the impact of exceeding those thresholds that resulted in increased MRSA rates. The study was undertaken in a 700-bed hospital in England between January 2015 and December 2021 (84 monthly observations). By employing the threshold logistic modelling approach, we: (i) determined the cut-off percentile value of MRSA incidence that defines a critical level of MRSA; (ii) identified thresholds for fluoroquinolone and co-amoxiclav use that would accelerate MRSA incidence rates and increase the probability of reaching critical incidence levels; (iii) enabled a better understanding of the effect of antibiotic use on the probability of reaching a critical level of resistant pathogen incidence; (iv) developed a near real-time performance monitoring feedback system; (v) provided risk scores and alert signals for antibiotic use, with the ability to inform hospital policies, and control MRSA incidence; and (vi) provided recommendations and an example for the management of pathogen incidence in hospitals. Threshold logistic models can help hospitals determine quantitative targets for antibiotic usage and can also inform effective antimicrobial stewardship to control resistance in hospitals. Studies should work toward implementing and evaluating the proposed approach prospectively, with the aim of determining the best counter-measures to mitigate the risk of increased resistant pathogen incidence in hospitals.
In Situ Biosynthesis of Reduced Alpha Hematite (α-Fe2O3) Nanoparticles by Stevia Rebaudiana L. Leaf Extract: Insights into Antioxidant, Antimicrobial, and Anticancer Properties
Authors: Alshawwa Samar Zuhair , Mohammed Eman J. , Hashim Nada , Sharaf Mohamed , Selim Samy , Alhuthali Hayaa M. , Alzahrani Hind A. , Mekky Alsayed E. , Elharrif Mohamed G.
Published: September 2022

In the present study, we utilized Stevia rebaudiana L. (SRLe) extract to in situ biosynthesize nanoscale alpha hematite (α-Fe2O3) nanoparticles (NPs) with potent antioxidant, antimicrobial, and anticancer properties. SRLe-α-Fe2O3 was characterized using physiochemical analyses, including UV/Vis, FTIR, XRD, DLS, EDX, SEM, and TEM studies. Among tested solvents, CHCl3/MeOH (2:1 v/v) SRL extract (least polar solvent) contained the highest EY, TPC, and antioxidant capacity of ~3.5%, ~75 mg GAE/g extract, and IC50 = 9.87 ± 0.7 mg/mL, respectively. FTIR confirmed the engagement of coating operation to the colloidal α-Fe2O3 NPs. TEM, SEM, and DLS revealed that SRLe-α-Fe2O3 has a spherical shape, uniform size distribution with aggregation for an average size of ~18.34 nm, and ζ = −19.4 mV, forming a repulsive barrier that helped to improve stability. The synthesized nanoparticles displayed considerable antibacterial activity against E. coli and S. aureus bacterial growth, and exhibited superior activity against the A549 lung cancer cell lines. These findings indicate that the increased availability of bioactive substances with antioxidant properties of SRLe makes it a potentially interesting material for the preparation of biologically active compounds and green synthesis of nanoparticles.
First Belgian Report of Ertapenem Resistance in an ST11 Klebsiella Pneumoniae Strain Isolated from a Dog Carrying blaSCO-1 and blaDHA-1 Combined with Permeability Defects
Authors: Debergh Hanne , Maex Margo , Garcia-Graells Cristina , Boland Cécile , Saulmont Marc , Van Hoorde Koenraad , Saegerman Claude
Published: September 2022

Klebsiella pneumoniae of sequence type (ST) 11 is a hyper-epidemic nosocomial clone, which is spreading worldwide among humans and emerging in pets. This is the first report, to the best of our knowledge, of multidrug-resistant (MDR) K. pneumoniae ST11 carrying blaSCO-1 and blaDHA-1, isolated from a four-month-old dog in Belgium. Antimicrobial susceptibility testing (AST) of the isolate, performed via broth microdilution following the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines, revealed resistance to eight different classes of antimicrobials, including carbapenems, in particular ertapenem, third-generation cephalosporins and fluoroquinolones. A hybrid approach, combining long- and short-read sequencing, was employed for in silico plasmid characterization, multi-locus sequence typing (MLST) and the identification and localization of antimicrobial resistance (AMR) and virulence-associated genes. Three plasmids were reconstructed from the whole-genome sequence (WGS) data: the conjugative IncFIB(K), the non-mobilizable IncR and the mobilizable but unconjugative ColRNAI. The IncFIB(K) plasmid carried the blaSCO-1 gene, whereas IncR carried blaDHA-1, both alongside several other antimicrobial resistance genes (ARGs). No virulence genes could be detected. Here, we suggest that the resistance to ertapenem associated with susceptibility to imipenem and meropenem in K. pneumoniae could be related to the presence of blaSCO-1 and blaDHA-1, combined with permeability defects caused by point mutations in an outer membrane porin (OmpK37). The presence of the blaSCO-1 gene on a conjugative IncFIB(K) plasmid is worrisome as it can increase the risk of transmission to humans, to animals and to the environment.
Dual Infection of an Open Fracture Caused by Mycobacterium setense and Clostridium celerecrescens
Authors: Ryskova Lenka , Zahradnicek Jan , Kukla Rudolf , Bolehovska Radka , Vajda Milan , Pavlik Ivo , Bostik Pavel , Ryska Pavel
Published: September 2022

Infections caused by Mycobacterium setense or Clostridium celerecrescens are extremely rare. In this report, for the first time a dual infection with these two pathogens is described. An 18-year-old female suffered multiple injuries, including an open comminuted fracture of the right humeral diaphysis after falling from a fifth-floor balcony in January 2019. Five months after the accident, a fistula appeared in the scar, reaching the bone tissue. M. setense and C. celerecrescens were cultured from sinus swabs and subsequently from perioperative samples. The patient was initially treated with a combination of intravenous antibiotics (ATBs): imipenem, amikacin, and ciprofloxacin. One month after the fracture fixation with a titanium nail, C. celerecrescens was again detected; therefore, metronidazole was added to the therapy. A triple combination of oral (PO) ATBs (trimethoprim–sulfamethoxazole, moxifloxacin, and metronidazole) followed, 8 weeks after the initial intravenous therapy. C. celerecrescens was cultured again two times, most recently in November 2019, when surgical debridement was supplemented by the topical administration of cancellous bone impregnated with vancomycin. Signs of bone healing were found at follow-ups and ATB treatment was finished in March 2020 after a total of 9 months of therapy. To this day, there have been no signs of reinfection. This case thus illustrates the need for a combination of systemic and individualized local therapy in the treatment of complicated cases of dual infections with rare pathogens.
Chemopreventive Properties of Black Raspberries and Strawberries in Esophageal Cancer Review
Authors: Shi Ni , Chen Tong
Published: September 2022

Esophageal cancer is one of the most fetal malignancies in the world. Esophageal squamous cell carcinoma (SCC) and esophageal adenocarcinoma (AC) are two main types of esophageal cancer and each with distinct epidemiological, etiological and histopathological characteristics. The continued global prevalence of tobacco use and alcohol consumption, coupled with limited intake of fresh fruits and vegetables, ensures that esophageal cancer will remain one of the major health threats. In addition to promoting quitting smoking and alcohol abuse, one of the strategies of cancer prevention is to identify foods, food components, or dietary patterns that can prevent or delay the onset of esophageal cancer. A food-based approach has the advantage of a complex of mixtures of bioactive components simultaneously targeting multiple processes in carcinogenesis. We have employed a preclinical rodent model of esophageal SCC to assess the effects of black raspberries (BRB) and strawberries. Our investigations demonstrate that BRB and strawberries are potent inhibitors of esophageal cancer. To prepare for this review, a literature search was performed to screen BRB and strawberries against esophageal cancer using electronic databases from PubMed, Science Direct and Google Scholar. Search was conducted covering the period from January 2000 to June 2022. Our present review has provided a systematic review about chemopreventive effects of BRB and strawberries in esophageal cancer by collecting and compiling diverse research findings from the above sources. In this review, we discussed the anti-tumor potentials of BRB and strawberries in esophageal SCC and esophageal AC separately. For each cancer type, we discuss animal models and research findings from both animal bioassays and human clinical studies. We also discuss the potential mechanisms of action of berries and their key bioactive components.
Modulating Nitric Oxide Dioxygenase and Nitrite Reductase of Cytoglobin through Point Mutations
Authors: Ukeri John , Wilson Michael T. , Reeder Brandon J.
Published: September 2022

Cytoglobin is a hexacoordinate hemoglobin with physiological roles that are not clearly understood. Previously proposed physiological functions include nitric oxide regulation, oxygen sensing, or/and protection against oxidative stress under hypoxic/ischemic conditions. Like many globins, cytoglobin rapidly consumes nitric oxide under normoxic conditions. Under hypoxia, cytoglobin generates nitric oxide, which is strongly modulated by the oxidation state of the cysteines. This gives a plausible role for this biochemistry in controlling nitric oxide homeostasis. Mutations to control specific properties of hemoglobin and myoglobin, including nitric oxide binding/scavenging and the nitrite reductase activity of various globins, have been reported. We have mapped these key mutations onto cytoglobin, which represents the E7 distal ligand, B2/E9 disulfide, and B10 heme pocket residues, and examined the nitric oxide binding, nitric oxide dioxygenase activity, and nitrite reductase activity. The Leu46Trp mutation decreases the nitric oxide dioxygenase activity > 10,000-fold over wild type, an effect 1000 times greater than similar mutations with other globins. By understanding how particular mutations can affect specific reactivities, these mutations may be used to target specific cytoglobin activities in cell or animal models to help understand the precise role(s) of cytoglobin under physiological and pathophysiological conditions.
Anti- and Pro-Oxidant Properties of Essential Oils against Antimicrobial Resistance
Authors: Kong Amanda Shen-Yee , Maran Sathiya , Yap Polly Soo-Xi , Lim Swee-Hua Erin , Yang Shun-Kai , Cheng Wan-Hee , Tan Yong-Hui , Lai Kok-Song
Published: September 2022

The rapid evolution of antimicrobial resistance (AMR) has remained a major public health issue, reducing the efficacy of antibiotics and increasing the difficulty of treating infections. The discovery of novel antimicrobial agents is urgently needed to overcome the challenges created by AMR. Natural products such as plant extracts and essential oils (EOs) have been viewed as potential candidates to combat AMR due to their complex chemistry that carries inherent pro-oxidant and antioxidant properties. EOs and their constituents that hold pro-oxidant properties can induce oxidative stress by producing reactive oxygen species (ROS), leading to biological damage in target cells. In contrast, the antioxidant properties scavenge free radicals through offsetting ROS. Both pro-oxidant and antioxidant activities in EOs represent a promising strategy to tackle AMR. Thus, this review aimed to discuss how pro-oxidants and antioxidants in EOs may contribute to the mitigation of AMR and provided a detailed description of the challenges and limitations of utilizing them as a means to combat AMR.
Sequential Accumulation of ‘Driver’ Pathway Mutations Induces the Upregulation of Hydrogen-Sulfide-Producing Enzymes in Human Colonic Epithelial Cell Organoids
Authors: Ascenção Kelly , Dilek Nahzli , Zuhra Karim , Módis Katalin , Sato Toshiro , Szabo Csaba
Published: September 2022

Recently, a CRISPR-Cas9 genome-editing system was developed with introduced sequential ‘driver’ mutations in the WNT, MAPK, TGF-β, TP53 and PI3K pathways into organoids derived from normal human intestinal epithelial cells. Prior studies have demonstrated that isogenic organoids harboring mutations in the tumor suppressor genes APC, SMAD4 and TP53, as well as the oncogene KRAS, assumed more proliferative and invasive properties in vitro and in vivo. A separate body of studies implicates the role of various hydrogen sulfide (H2S)-producing enzymes in the pathogenesis of colon cancer. The current study was designed to determine if the sequential mutations in the above pathway affect the expression of various H2S producing enzymes. Western blotting was used to detect the expression of the H2S-producing enzymes cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3-MST), as well as several key enzymes involved in H2S degradation such as thiosulfate sulfurtransferase/rhodanese (TST), ethylmalonic encephalopathy 1 protein/persulfide dioxygenase (ETHE1) and sulfide-quinone oxidoreductase (SQR). H2S levels were detected by live-cell imaging using a fluorescent H2S probe. Bioenergetic parameters were assessed by Extracellular Flux Analysis; markers of epithelial-mesenchymal transition (EMT) were assessed by Western blotting. The results show that the consecutive mutations produced gradual upregulations in CBS expression—in particular in its truncated (45 kDa) form—as well as in CSE and 3-MST expression. In more advanced organoids, when the upregulation of H2S-producing enzymes coincided with the downregulation of the H2S-degrading enzyme SQR, increased H2S generation was also detected. This effect coincided with the upregulation of cellular bioenergetics (mitochondrial respiration and/or glycolysis) and an upregulation of the Wnt/β-catenin pathway, a key effector of EMT. Thus sequential mutations in colon epithelial cells according to the Vogelstein sequence are associated with a gradual upregulation of multiple H2S generating pathways, which, in turn, translates into functional changes in cellular bioenergetics and dedifferentiation, producing more aggressive and more invasive colon cancer phenotypes.
Advances in Natural Antioxidants for Food Improvement
Authors: López-Pedrouso María , Lorenzo José M. , Franco Daniel
Published: September 2022

In the food industry, antioxidants are natural and synthetic compounds added to neutralize free radicals that deteriorate fats, proteins and cellular DNA, causing rancidity of fats and accelerating the ageing process, which lead to undesirable smells and tastes [...]
Estimating Infection-Related Human Mobility Networks Based on Time Series Data of COVID-19 Infection in Japan
Authors: Yamada Tetsuya , Shi Shoi
Published: September 2022

Background: Comprehensive and evidence-based countermeasures against emerging infectious diseases have become increasingly important in recent years. COVID-19 and many other infectious diseases are spread by human movement and contact, but complex transportation networks in the 21st century make it difficult to predict disease spread in rapidly changing situations. It is especially challenging to estimate the network of infection transmission in countries where traffic and human movement data infrastructure is not yet developed. Methods: In this study, we devised a method utilizing an ordinary and partial differential equations-based mathematical model and a modified mathematical optimization method to estimate the network of transmission of COVID-19 from the time series data of its infection and applied it to determine its spread across areas in Japan. Furthermore, utilizing the estimated human mobility network, we predicted the spread of infection using the Tokyo Olympics as a model. Findings: We incorporated the effects of soft lockdowns, such as the declaration of a state of emergency, and changes in the infection network due to government-sponsored travel promotion, and revealed that the estimated effective distance captured human mobility changing dynamically in the different stages of the pandemic. The model predicted that the Tokyo Olympic and Paralympic Games would increase the number of infected cases in the host prefectures by up to 80%. Interpretation: The models used in this study are available online, and our data-driven infection network models are scalable, whether it be at the level of a city, town, country, or continent, and applicable anywhere in the world, as long as the time-series data of infections per region is available. These estimations of effective distance and the depiction of infectious disease networks based on actual infection data are expected to be useful in devising data-driven countermeasures against emerging infectious diseases worldwide.
Fluorimetry in the Strong-Coupling Regime: From a Fundamental Perspective to Engineering New Tools for Tracing and Marking Materials and Objects
Authors: Hatifi Mohamed , Mara Dimitrije , Bokic Bojana , Van Deun Rik , Stout Brian , Lassalle Emmanuel , Kolaric Branko , Durt Thomas
Published: September 2022

Under exceptional circumstances, light and molecules bond together, creating new hybrid light–matter states with far-reaching consequences for these strongly coupled entities. The present article describes the quantum-mechanical foundation of strong-coupling and experimental evidence for molding the radiation properties of nanoprobes by strong-coupling. When applied to tracing and marking, the new fluorometry technique proposed here, which harnesses strong-coupling, has a triple advantage compared to its classical counterparts such as DNA tracing. It is fast, and its signal-to-noise ratio can be improved by spectral filtering; moreover, it reveals a specific quantum signature of the strong-coupling, which is extremely difficult to reproduce classically, thereby opening the door to new anti-counterfeiting strategies.
Corpus for Development of Routing Algorithms in Opportunistic Networks
Authors: Freire Diego , Borrego Carlos , Robles Sergi
Published: September 2022

We have designed a collection of scenarios, a corpus, for its use in the study and development of routing algorithms for opportunistic networks. To obtain these scenarios, we have followed a methodology based on characterizing the space and choosing the best exemplary items in such a way that the corpus as a whole was representative of all possible scenarios. Until now, research in this area was using some sets of non-standard network traces that made it difficult to evaluate algorithms and perform fair comparisons between them. These developments were hard to assess in an objective way, and were prone to introduce unintentional biases that directly affected the quality of the research. Our contribution is more than a collection of scenarios; our corpus provides a fine collection of network behaviors that suit the development of routing algorithms, specifically in evaluating and comparing them. If the scientific community embraces this corpus, the community will have a global-agreed methodology where the validity of results would not be limited to specific scenarios or network conditions, thus avoiding self-produced evaluation setups, availability problems and selection bias, and saving time. New research in the area will be able to validate the routing algorithms already published. It will also be possible to identify the scenarios better suit specific purposes, and results will be easily verified. The corpus is available free to download and use.
Occupancy Reward-Driven Exploration with Deep Reinforcement Learning for Mobile Robot System
Authors: Kamalova Albina , Lee Suk Gyu , Kwon Soon Hak
Published: September 2022

This paper investigates the solution to a mobile-robot exploration problem following autonomous driving principles. The exploration task is formulated in this study as a process of building a map while a robot moves in an indoor environment beginning from full uncertainties. The sequence of robot decisions of how to move defines the strategy of the exploration that this paper aims to investigate, applying one of the Deep Reinforcement Learning methods, known as the Deep Deterministic Policy Gradient (DDPG) algorithm. A custom environment is created representing the mapping process with a map visualization, a robot model, and a reward function. The actor-critic network receives and sends input and output data, respectively, to the custom environment. The input is the data from the laser sensor, which is equipped on the robot. The output is the continuous actions of the robot in terms of linear and angular velocities. The training results of this study show the strengths and weaknesses of the DDPG algorithm for the robotic mapping problem. The implementation was developed in MATLAB platform using its corresponding toolboxes. A comparison with another exploration algorithm is also provided.
Re-Calibration and Lens Array Area Detection for Accurate Extraction of Elemental Image Array in Three-Dimensional Integral Imaging
Authors: Jeong Hyeonah , Lee Eunsu , Yoo Hoon
Published: September 2022

This paper presents a new method for extracting an elemental image array in three-dimensional (3D) integral imaging. To reconstruct 3D images in integral imaging, as the first step, a method is required to accurately extract an elemental image array from a raw captured image. Thus, several methods have been discussed to extract an elemental image array. However, the accuracy is sometimes degraded due to inaccurate edge detection, image distortions, optical misalignment, and so on. Especially, small pixel errors can deteriorate the performance of an integral imaging system with a lens array. To overcome the problem, we propose a postprocessing method for the accurate extraction of an elemental image array. Our method is a unified version of an existing method and proposed postprocessing techniques. The proposed postprocessing consists of re-calibration and lens array area detection. Our method reuses the results from an existing method, and it then improves the results via the proposed postprocessing techniques. To evaluate the proposed method, we perform optical experiments for 3D objects and provide the resulting images. The experimental results indicate that the proposed postprocessing techniques improve an existing method for extracting an elemental image array in integral imaging. Therefore, we expect the proposed techniques to be applied to various applications of integral imaging systems
Comparative Study Using CAD Optimization Tools for the Workspace of a 6DOF Parallel Kinematics Machine
Authors: Stan Sergiu-Dan , Popişter Florin , Oarcea Alexandru , Ciudin Paul
Published: September 2022

This paper deals with an up-to-date topic among robotic industrial applications that require a high degree of speed, rigidity, and orientation. Currently, when technology and software applications reach a high level of performance, in various robotic industrial applications that start from certain concepts, the implementation of efficient structures has proven to be challenging. New structures such as the parallel kinematic machine (PKM) category has proven its efficiency through its structure in terms of high inertia rigidity and high speeds during processes. This paper deals with the subject of PKM-type structures in terms of the optimal design workspace of such a structure. The calculation of the workspace is considered the premise from which it starts in terms of its implementation in a robotic production line. The entire process of calculating the workspace for a given PKM structure is carried out through modern CAD applications that have specific modules in place in this direction. CATIA V5 offers the possibility through the product engineering optimizer module, simulation and calculation of different scenarios aimed at identifying the volume of the workspace for a PKM structure. In the article, we demonstrate the relations between the robot workspace and the design parameters, a method that can also be applied for other parallel structures. The method is useful for robot designers in the optimization of parallel robots with regard to the workspace by using CAD tools. Previous research in the field refers of the usage of CAD tools only for visual representation and not for optimizing the workspace, while this study and test results show that CAD tools are suitable for analyzing and optimizing the robot workspace of the 6DOF parallel robot, due to its easiness in application and fast implementation time.
Functional and Sensory Properties of Gingerbread Enriched with the Addition of Vegetables
Authors: Przybylski Wiesław , Jaworska Danuta , Sionek Barbara , Sankowska Weronika , Wójtowicz Marta
Published: September 2022

The aim of the study was to evaluate the sensory quality and bioactive phenolic compounds and antioxidant activity of gingerbread cake with the addition of different vegetables, which was separately prepared as studied samples. The research material consisted of three gingerbread banana cakes and various vegetable ingredients: pumpkin (Cucurbita pepo L.), tomato (Solanum lycopersicum L.), and beetroot (Beta vulgaris L.). Blended roasted pumpkin, blended roasted beetroots, and baked tomatoes were used in the experiment. The evaluation of sensory quality was conducted using the QDP (Quantitative Descriptive Profiling) method and a hedonic evaluation by consumers was also performed. In the experiment, colour parameters were measured in the CIE L*a*b* system, and the total polyphenol content and the total antioxidant capacity were established. The test results showed that a vegetable addition of a level of up to 25% of different vegetables such as pumpkin, tomato, and beetroot to gingerbread provides an adequate effect on the sensory quality of the product while still being acceptable to consumers. Moreover, it increases the functional properties of the finished product (p < 0.01). The gingerbread dough with the addition of tomato (25%) contained the highest amount of polyphenols −40 mg GAE/100 g, and the gingerbread dough with the pumpkin addition was characterized by the highest total antioxidant activity at a mean value of 0.475 μM TEAC/g of product.
Al-RHEA Particulates MMCs by PM Route: Mechanical Properties and Sliding Wear Response
Authors: Ananiadis Elias Anastasios , Karantzalis Alexander Efstathios , Exarchos Dimitrios A. , Matikas Theodore E.
Published: September 2022

New particle reinforced aluminum matrix composites with the addition of refractory High Entropy Alloy, MoTaNbVW, fabricated via powder metallurgy process were assessed for their properties. Basic mechanical properties (modulus of elasticity, hardness) for the aluminum matrix, the pure aluminum and the reinforcement phase were assessed by means of dynamic nano-indentation technique. Nano-indentation based creep response was also evaluated in these three areas of interest. Hardness shows an increase with the addition of the particulates and so does the elastic moduli and the ratio of the energy absorbed in the elastic region. The creep response was approached in terms of dislocation mobility and critical volume for their nucleation. The produced Al–HEA composites were also studied for their sliding wear behavior and showed that with the increase in percentage of RHEA particulates the wear resistance increases. Microstructural considerations, wear track morphologies, and debris characteristics were used for the assessment of the involved wear mechanisms.
Surgical Classification for Preclinical Rat Femoral Bone Defect Model: Standardization Based on Systematic Review, Anatomical Analysis and Virtual Surgery
Authors: Sun Yu , Helmholz Heike , Willumeit-Römer Regine
Published: September 2022

Though surgical techniques profoundly influence in vivo experiments, significant heterogeneity exists in current surgeries for inducing rat femoral bone defects. Such variations reduce the reproducibility and comparability of preclinical studies, and are detrimental to clinical translation. The purposes of this study were: (1) to conduct a systematic review of rat femoral defect models, summarizing and analyzing the surgical techniques; (2) to analyze surgical design and potential pitfalls via 3D anatomy and virtual surgeries for fostering future precision research; and (3) to establish a surgical classification system, for improving the reproducibility and comparability among studies, avoiding unnecessary repetitive experiments. The online database PubMed was searched to identify studies from January 2000 to June 2022 using keywords, including rat, femur, bone defect. Eligible publications were included for a review of surgical methods. Anatomical analysis and virtual surgeries were conducted based on micro-CT reconstruction of the rat femur for further investigation and establishment of a classification system. A total of 545 publications were included, revealing marked heterogeneity in surgical methods. Four major surgical designs were reported for inducing defects from the proximal to distal femur: bone tunnel, cortical window, segmental defect, and wedge-shaped defect. Anatomical analysis revealed potential pitfalls hindering efficient clinical translation. A classification system was established according to the anatomical region, surgical design, and fixation devices. This systematic review in combination with 3D analysis and virtual surgery provides a general overview of current surgical approaches to inducing femoral defects in rats, and establishes a surgical classification facilitating preclinical research of quality and translational value.
Therapeutic Applications of the CRISPR-Cas System
Authors: Kang Kyungmin , Song Youngjae , Kim Inho , Kim Tae-Jung
Published: September 2022

The clustered regularly interspaced palindromic repeat (CRISPR)-Cas system has revolutionized genetic engineering due to its simplicity, stability, and precision since its discovery. This technology is utilized in a variety of fields, from basic research in medicine and biology to medical diagnosis and treatment, and its potential is unbounded as new methods are developed. The review focused on medical applications and discussed the most recent treatment trends and limitations, with an emphasis on CRISPR-based therapeutics for infectious disease, oncology, and genetic disease, as well as CRISPR-based diagnostics, screening, immunotherapy, and cell therapy. Given its promising results, the successful implementation of the CRISPR-Cas system in clinical practice will require further investigation into its therapeutic applications.
Calabria as a Genetic Isolate: A Model for the Study of Neurodegenerative Diseases
Authors: Bruno Francesco , Laganà Valentina , Di Lorenzo Raffaele , Bruni Amalia C. , Maletta Raffaele
Published: September 2022

Although originally multi-ethnic in its structure, nowadays the Calabria region of southern Italy represents an area with low genetic heterogeneity and a high level of consanguinity that allows rare mutations to be maintained due to the founder effect. A complex research methodology—ranging from clinical activity to the genealogical reconstruction of families/populations across the centuries, the creation of databases, and molecular/genetic research—was modelled on the characteristics of the Calabrian population for more than three decades. This methodology allowed the identification of several novel genetic mutations or variants associated with neurodegenerative diseases. In addition, a higher prevalence of several hereditary neurodegenerative diseases has been reported in this population, such as Alzheimer’s disease, frontotemporal dementia, Parkinson’s disease, Niemann–Pick type C disease, spinocerebellar ataxia, Creutzfeldt–Jakob disease, and Gerstmann–Straussler–Scheinker disease. Here, we summarize and discuss the results of research data supporting the view that Calabria could be considered as a genetic isolate and could represent a model, a sort of outdoor laboratory—similar to very few places in the world—useful for the advancement of knowledge on neurodegenerative diseases.
Saturated Fatty Acid Blood Levels and Cardiometabolic Phenotype in Patients with HFpEF: A Secondary Analysis of the Aldo-DHF Trial
Authors: Lechner Katharina , von Schacky Clemens , Scherr Johannes , Lorenz Elke , Bock Matthias , Lechner Benjamin , Haller Bernhard , Krannich Alexander , Halle Martin , Wachter Rolf , Duvinage André , Edelmann Frank
Published: September 2022

Background: Circulating long-chain (LCSFAs) and very long-chain saturated fatty acids (VLSFAs) have been differentially linked to risk of incident heart failure (HF). In patients with heart failure with preserved ejection fraction (HFpEF), associations of blood SFA levels with patient characteristics are unknown. Methods: From the Aldo-DHF-RCT, whole blood SFAs were analyzed at baseline in n = 404 using the HS-Omega-3-Index® methodology. Patient characteristics were 67 ± 8 years, 53% female, NYHA II/III (87%/13%), ejection fraction ≥50%, E/e’ 7.1 ± 1.5; and median NT-proBNP 158 ng/L (IQR 82–298). Spearman´s correlation coefficients and linear regression analyses, using sex and age as covariates, were used to describe associations of blood SFAs with metabolic phenotype, functional capacity, cardiac function, and neurohumoral activation at baseline and after 12-month follow-up (12 mFU). Results: In line with prior data supporting a potential role of de novo lipogenesis-related LCSFAs in the development of HF, we showed that baseline blood levels of C14:0 and C16:0 were associated with cardiovascular risk factors and/or lower exercise capacity in patients with HFpEF at baseline/12 mFU. Contrarily, the three major circulating VLSFAs, lignoceric acid (C24:0), behenic acid (C22:0), and arachidic acid (C20:0), as well as the LCSFA C18:0, were broadly associated with a lower risk phenotype, particularly a lower risk lipid profile. No associations were found between cardiac function and blood SFAs. Conclusions: Blood SFAs were differentially linked to biomarkers and anthropometric markers indicative of a higher-/lower-risk cardiometabolic phenotype in HFpEF patients. Blood SFA warrant further investigation as prognostic markers in HFpEF. One Sentence Summary: In patients with HFpEF, individual circulating blood SFAs were differentially associated with cardiometabolic phenotype and aerobic capacity.
Consecutive Inhibition of Telomerase and Alternative Lengthening Pathway Promotes Hodgkin’s Lymphoma Cell Death
Authors: Lima Matheus Fabiao de , Freitas Monique Oliveira , Hamedani Mohammad K. , Rangel-Pozzo Aline , Zhu Xu-Dong , Mai Sabine
Published: September 2022

Telomere maintenance is key during cancer development. Malignant cells can either use telomerase or an alternative lengthening of telomere (ALT) pathway to maintain their telomere length. In Hodgkin’s Lymphoma (HL), the presence of telomerase activation is established. The activation of ALT has been reported recently. Our data confirm this notion describing co-localization of the phosphorylated form of telomeric repeat-binding factor 1 (pT371-TRF1) with ALT-associated promyelocytic leukemia bodies. Surprisingly, to our knowledge, there are no published studies targeting both telomere maintenance pathways in HL. Consequently, we investigated, for the first time, the effects of both telomerase and ALT inhibition on HL cell viability: We inhibited telomerase and/or ALT, given either individually, simultaneously, or consecutively. We report that the inhibition of telomerase using BIBR1532 followed by ALT inhibition, using trabectedin, caused a decrease of greater than 90% in cell viability in three patient-derived HL cell lines. Our results suggest that HL cells are most vulnerable to the consecutive inhibition of telomerase followed by ALT inhibition.
Pinus halepensis Essential Oil Ameliorates Aβ1-42-Induced Brain Injury by Diminishing Anxiety, Oxidative Stress, and Neuroinflammation in Rats
Authors: Postu Paula Alexandra , Mihasan Marius , Gorgan Dragos Lucian , Sadiki Fatima Zahra , El Idrissi Mostafa , Hritcu Lucian
Published: September 2022

The Pinus L. genus comprises around 250 species, being popular worldwide for their medicinal and aromatic properties. The present study aimed to evaluate the P. halepensis Mill. essential oil (PNO) in an Alzheimer’s disease (AD) environment as an anxiolytic and antidepressant agent. The AD-like symptoms were induced in Wistar male rats by intracerebroventricular administration of amyloid beta1-42 (Aβ1-42), and PNO (1% and 3%) was delivered to Aβ1-42 pre-treated rats via inhalation route for 21 consecutive days, 30 min before behavioral assessments. The obtained results indicate PNO’s potential to relieve anxious–depressive features and to restore redox imbalance in the rats exhibiting AD-like neuropsychiatric impairments. Moreover, PNO presented beneficial effects against neuroinflammation and neuroapoptosis in the Aβ1-42 rat AD model.
Multi-Channel Metabolomics Analysis Identifies Novel Metabolite Biomarkers for the Early Detection of Fatty Liver Disease in Dairy Cows
Authors: Zhang Xuan , Liu Tingjun , Hou Xianpeng , Hu Chengzhang , Zhang Letian , Wang Shengxuan , Zhang Qin , Shi Kerong
Published: September 2022

Fatty liver disease, a type of metabolic disorder, frequently occurs in dairy cows during the parturition period, causing a high culling rate and, therefore, considerable economic losses in the dairy industry owing to the lack of effective diagnostic methods. Here, metabolite biomarkers were identified and validated for the diagnosis of metabolic disorders. A total of 58 participant cows, including severe fatty liver disease and normal control groups, in the discovery set (liver biopsy tested, n = 18), test set (suspected, n = 20) and verification set (liver biopsy tested, n = 20), were strictly recruited and a sample collected for their feces, urine, and serum. Non-targeted GC-MS-based metabolomics methods were used to characterize the metabolite profiles and to screen in the discovery set. Eventually, ten novel biomarkers involved in bile acid, amino acid, and fatty acid were identified and validated in the test set. Each of them had a higher diagnostic ability than the traditional serum biochemical indicators, with an average area under the receiver operating characteristic curve of 0.830 ± 0.0439 (n = 10) versus 0.377 ± 0.182 (n = 9). Especially, combined biomarker panels via different metabolic pipelines had much better diagnostic sensitivity and specificity than every single biomarker, suggesting their powerful utilization potentiality for the early detection of fatty liver disease. Intriguingly, the serum biomarkers were confirmed perfectly in the verification set. Moreover, common biological pathways were found to be underlying the pathogenesis of fatty liver syndrome in cattle via different metabolic pipelines. These newly-discovered and non-invasive metabolic biomarkers are meaningful in reducing the high culling rate of cows and, therefore, benefit the sustainable development of the dairy industry.
A Review of the Pharmacological Potential of Spatholobus suberectus Dunn on Cancer
Authors: Zhang Feng , Ganesan Kumar , Liu Qingqing , Chen Jianping
Published: September 2022

Spatholobus suberectus Dunn (SSD) has been extensively employed in Traditional Chinese Medicine to treat several ailments. SSD and its active compounds are effective therapeutic agents for treating a variety of diseases with negligible side effects. Therefore, we aimed to investigate its phytochemistry, pharmacology, and potential therapeutic effects exclusively in cancer prevention and treatment. Phytochemical and pharmacological information was collected and arranged in a rational order. SSD has been frequently attributed to having antioxidant, anti-diabetic, anti-inflammatory, hematopoietic, neuroprotective, antimicrobial, and anticancer properties. Evidence has indicated that the bioactive constituents in SSD have attracted increasing scientific attention due to their preventive role in cancers. Further, the present review provides the current information on the health implications of SSD, thus allowing for future clinical trials to explore its restorative benefits. All data of in vitro and animal investigations of SSD, as well as its effect on human health, were obtained from an electronic search and library database. The diverse pharmacological potential of SSD provides an opportunity for preclinical drug discovery, and this comprehensive review strongly indicates that SSD is an excellent anti-tumorigenic agent that modulates or prevents breast cancer.
A Charcot-Marie-Tooth-Causing Mutation in HSPB1 Decreases Cell Adaptation to Repeated Stress by Disrupting Autophagic Clearance of Misfolded Proteins
Authors: Zhang Xuelian , Qiao Yaru , Han Ronglin , Gao Yingjie , Yang Xun , Zhang Ying , Wan Ying , Yu Wei , Pan Xianchao , Xing Juan
Published: September 2022

Charcot-Marie-Tooth (CMT) disease is the most common inherited neurodegenerative disorder with selective degeneration of peripheral nerves. Despite advances in identifying CMT-causing genes, the underlying molecular mechanism, particularly of selective degeneration of peripheral neurons remains to be elucidated. Since peripheral neurons are sensitive to multiple stresses, we hypothesized that daily repeated stress might be an essential contributor to the selective degeneration of peripheral neurons induced by CMT-causing mutations. Here, we mainly focused on the biological effects of the dominant missense mutation (S135F) in the 27-kDa small heat-shock protein HSPB1 under repeated heat shock. HSPB1S135F presented hyperactive binding to both α-tubulin and acetylated α-tubulin during repeated heat shock when compared with the wild type. The aberrant interactions with tubulin prevented microtubule-based transport of heat shock-induced misfolded proteins for the formation of perinuclear aggresomes. Furthermore, the transport of autophagosomes along microtubules was also blocked. These results indicate that the autophagy pathway was disrupted, leading to an accumulation of ubiquitinated protein aggregates and a significant decrease in cell adaptation to repeated stress. Our findings provide novel insights into the molecular mechanisms of HSPB1S135F-induced selective degeneration of peripheral neurons and perspectives for targeting autophagy as a promising therapeutic strategy for CMT neuropathy.