bluuebunny/crossref_metadata_2025 huggingface dataset:
Associations between Fasting Duration, Timing of First and Last Meal, and Cardiometabolic Endpoints in the National Health and Nutrition Examination Survey
Authors: Wirth Michael D. , Zhao Longgang , Turner-McGrievy Gabrielle M. , Ortaglia Andrew
Published: August 2021

Background: Research indicates potential cardiometabolic benefits of energy consumption earlier in the day. This study examined the association between fasting duration, timing of first and last meals, and cardiometabolic endpoints using data from the National Health and Nutrition Examination Survey (NHANES). Methods: Cross-sectional data from NHANES (2005–2016) were utilized. Diet was obtained from one to two 24-h dietary recalls to characterize nighttime fasting duration and timing of first and last meal. Blood samples were obtained for characterization of C-reactive protein (CRP); glycosylated hemoglobin (HbA1c %); insulin; glucose; and high-density lipoprotein (HDL), low-density lipoprotein (LDL), and total cholesterol. Survey design procedures for adjusted linear and logistic regression were performed. Results: Every one-hour increase in nighttime fasting duration was associated with a significantly higher insulin and CRP, and lower HDL. Every one-hour increase in timing of the last meal of the day was statistically significantly associated with higher HbA1c and lower LDL. Every one-hour increase in first mealtime was associated with higher CRP (β = 0.044, p = 0.0106), insulin (β = 0.429, p < 0.01), and glucose (β = 0.662, p < 0.01), and lower HDL (β = −0.377, p < 0.01). Conclusion: In this large public health dataset, evidence for the beneficial effect of starting energy consumption earlier in the day on cardiometabolic endpoints was observed.
Feeding Children and Maintaining Food Service Operations during COVID-19: A Mixed Methods Investigation of Implementation and Financial Challenges
Authors: Kenney Erica L. , Dunn Caroline G. , Mozaffarian Rebecca S. , Dai Jane , Wilson Katie , West Jeremy , Shen Ye , Fleischhacker Sheila , Bleich Sara N.
Published: August 2021

The United States Department of Agriculture (USDA) National School Lunch and Breakfast Programs are critical for the health and food security of U.S. schoolchildren, but access to these programs was disrupted by COVID-19 pandemic-related school closures in spring 2020. While temporary policy changes to the programs enabled school food authorities (SFAs) to pivot towards distributing meals throughout their communities instead of within school buildings, SFAs faced complex challenges during COVID-19 with minimal external support. This mixed methods study investigates the implementation and financial challenges experienced by twelve of the largest urban SFAs in the U.S. during COVID-19. We conducted semi-structured interviews with SFA leaders and analyzed alongside quantitative financial data. We found that SFAs reconfigured their usual operations with nearly no preparation time while simultaneously trying to keep staff from contracting COVID-19, accommodate stakeholders with sometimes competing priorities, and remain financially solvent. Because student participation was much lower than during regular times, and revenue is tied to the number of meals served, SFAs saw drastic decreases in revenue even as they carried regular operating costs. For future crises, disaster preparedness plans that help SFAs better navigate the switch to financially viable community distribution methods are needed.
Effect of the Interaction between Dietary Patterns and the Gastric Microbiome on the Risk of Gastric Cancer
Authors: Gunathilake Madhawa , Lee Jeonghee , Choi Il Ju , Kim Young-Il , Kim Jeongseon
Published: August 2021

We aimed to observe the combined effects of Gaussian graphical model (GGM)-derived dietary patterns and the gastric microbiome on the risk of gastric cancer (GC) in a Korean population. The study included 268 patients with GC and 288 healthy controls. Food intake was assessed using a 106-item semiquantitative food frequency questionnaire. GGMs were applied to derive dietary pattern networks. 16S rRNA gene sequencing was performed using DNA extracted from gastric biopsy samples. The fruit pattern network was inversely associated with the risk of GC for the highest vs. lowest tertiles in the total population (odds ratio (OR): 0.47; 95% confidence interval (CI): 0.28–0.77; p for trend = 0.003) and in females (OR: 0.38; 95% CI: 0.17–0.83; p for trend = 0.021). Males who had a low microbial dysbiosis index (MDI) and high vegetable and seafood pattern score showed a significantly reduced risk of GC (OR: 0.44; 95% CI: 0.22–0.91; p-interaction = 0.021). Females who had a low MDI and high dairy pattern score showed a significantly reduced risk of GC (OR: 0.23; 95% CI: 0.07–0.76; p-interaction = 0.018). Our novel findings revealed that vegetable and seafood pattern might interact with dysbiosis to attenuate the risk of GC in males, whereas the dairy pattern might interact with dysbiosis to reduce the GC risk in females.
Towards a More Sustainable Nutrition: Complementary Feeding and Early Taste Experiences as a Basis for Future Food Choices
Authors: Mazzocchi Alessandra , De Cosmi Valentina , Scaglioni Silvia , Agostoni Carlo
Published: August 2021

The concept of sustainable nutrition considers different fields: from human health to environmental, economic and socio-cultural aspects. Currently, in Europe, the diets that reflect the assumptions of the sustainable diet are the Mediterranean Diet and the New Nordic Diet. They both encourage the consumption of vegetable, organic and minimally processed foods, as well as regional, seasonal and Fair-Trade products, reducing the ecological impact of the production chain. These eating habits could be established starting from the prenatal period and from infancy during the complementary feeding stage, aiding children to accept of a more variable diet in terms of flavor, taste and texture. In particular, the positive parental role model is an effective method for improving a child’s diet and behaviors. Two healthy plates representing a sustainable diet in early infancy, at 6 and 24 months, are here proposed, in line with the “Planetary Health Diet” approved by the EAT-Lancet Commission. Our work aims to highlight how a sustainable diet is possible since infancy, since the introduction of solid foods.
Influence of Brewer’s Spent Grain Compounds on Glucose Metabolism Enzymes
Authors: Becker Daniela , Bakuradze Tamara , Hensel Marcel , Beller Simone , Yélamos Carolina Corral , Richling Elke
Published: August 2021

With a yearly production of about 39 million tons, brewer’s spent grain (BSG) is the most abundant brewing industry byproduct. Because it is rich in fiber and protein, it is commonly used as cattle feed but could also be used within the human diet. Additionally, it contains many bioactive substances such as hydroxycinnamic acids that are known to be antioxidants and potent inhibitors of enzymes of glucose metabolism. Therefore, our study aim was to prepare different extracts—A1-A7 (solid-liquid extraction with 60% acetone); HE1-HE6 (alkaline hydrolysis followed by ethyl acetate extraction) and HA1-HA3 (60% acetone extraction of alkaline residue)—from various BSGs which were characterized for their total phenolic (TPC) and total flavonoid (TFC) contents, before conducting in vitro studies on their effects on the glucose metabolism enzymes α-amylase, α-glucosidase, dipeptidyl peptidase IV (DPP IV), and glycogen phosphorylase α (GPα). Depending on the extraction procedures, TPCs ranged from 20–350 µg gallic acid equivalents/mg extract and TFCs were as high as 94 µg catechin equivalents/mg extract. Strong inhibition of glucose metabolism enzymes was also observed: the IC50 values for α-glucosidase inhibition ranged from 67.4 ± 8.1 µg/mL to 268.1 ± 29.4 µg/mL, for DPP IV inhibition they ranged from 290.6 ± 97.4 to 778.4 ± 95.5 µg/mL and for GPα enzyme inhibition from 12.6 ± 1.1 to 261 ± 6 µg/mL. However, the extracts did not strongly inhibit α-amylase. In general, the A extracts from solid-liquid extraction with 60% acetone showed stronger inhibitory potential towards a-glucosidase and GPα than other extracts whereby no correlation with TPC or TFC were observed. Additionally, DPP IV was mainly inhibited by HE extracts but the effect was not of biological relevance. Our results show that BSG is a potent source of α-glucosidase and GPα inhibitors, but further research is needed to identify these bioactive compounds within BSG extracts focusing on extracts from solid-liquid extraction with 60% acetone.
Effect of Temperature on the Expression of Classical Enterotoxin Genes among Staphylococci Associated with Bovine Mastitis
Authors: Homsombat Theeyathart , Boonyayatra Sukolrat , Awaiwanont Nattakarn , Pichpol Duangporn
Published: August 2021

Staphylococcal food poisoning (SFP), caused by the contamination of staphylococcal enterotoxins, is a common foodborne disease worldwide. The aims of this study were: (1) to investigate classical staphylococcal enterotoxin genes, sea, seb, sec, sed, and see, among Staphylococcus aureus and coagulase-negative staphylococci (CNS) associated with bovine mastitis; (2) to determine the effect of temperature on the expression of classical staphylococcal enterotoxin genes in staphylococci in milk. The detection of classical staphylococcal enterotoxin genes was performed using S. aureus (n = 51) and CNS (n = 47). The expression of classical enterotoxin genes, including sea, seb, sec, and see, was determined during the growth of staphylococci in milk subjected to ultra-high-temperature processing at two different temperatures: 8 °C and room temperature. Classical staphylococcal enterotoxin genes were expressed more frequently in S. aureus (35.30%) than in CNS (12.77%). The sec gene was most frequently detected in S. aureus (29.41%) and CNS (6.38%). Moreover, the expression of sea and sec was significantly higher at room temperature than at 8 °C after 16 h of incubation (p < 0.05). These results emphasize the importance of maintaining the storage temperature of milk below 8 °C to reduce the risk of SFP.
Critical Systematic Review of Zoonoses and Transboundary Animal Diseases’ Prioritization in Africa
Authors: Mpouam Serge Eugene , Mingoas Jean Pierre Kilekoung , Mouiche Mohamed Moctar Mouliom , Kameni Feussom Jean Marc , Saegerman Claude
Published: August 2021

Background: Disease prioritization aims to enhance resource use efficiency concerning human and animal health systems’ preparedness and response to the most important problems for the optimization of beneficial outcomes. In sub-Sahara Africa (SSA), several prioritizations of zoonoses and transboundary animal diseases (TADs) have been implemented at different scales to characterize potential disease impacts. Method and principal findings: In this systematic review, we analyze the methodologies used, outcomes, and their relevance by discussing criteria required to align decision-makers’ perceptions of impacts to those of other stakeholders for different prioritization in SSA. In general, the sectorial representativeness of stakeholders for processes implemented with the support of international partners showed slight differences with the absence of local stakeholders. Whatever the tool prioritized, zoonoses were similar in general because of the structured nature of those tools in assessing decision-makers’ preferences through value trade-offs between criteria while ensuring transparency and reproducibility. However, by involving field practitioners and farmers, there were different outcomes with processes concerning only decision makers and experts who were more sensitive to infectious TADs, while the former raised parasitic disease constraints. In this context, multicriteria decision analysis-based zoonoses and TADs prioritizations involving a balanced participation of stakeholders might contribute to bridging these divergences, whatever the scale. Conclusion and significance: Prioritization processes were important steps toward building and harmonizing technical laboratory and surveillance networks to coordinate projects to address priority zoonoses and TADs at the country and/or sub-regional level. Those processes should be enhanced.
In Vitro Testing of Alternative Synthetic and Natural Antiparasitic Compounds against the Monogenean Sparicotyle chrysophrii
Authors: Mladineo Ivona , Trumbić Željka , Ormad-García Adrián , Palenzuela Oswaldo , Sitjà-Bobadilla Ariadna , Manuguerra Simona , Ruiz Cristobal Espinosa , Messina Concetta Maria
Published: August 2021

Gill monogenean Sparicotyle chrysophrii is considered the most detrimental fish parasite to the Mediterranean aquaculture. Treatment of sparicotylosis relies on frequent gill inspections correlated with the seasonal increase in seawater temperature, application of functional feeds, and treatments with formalin baths where permitted. While the latter is bound to be banned in Europe, other synthetic anthelminthics, such as praziquantel and ivermectin, are prone to induce resistance in the parasites. Therefore, we investigated, in vitro, 14 synthetic and natural compounds against adult S. chrysophrii, developing dose–response modelsm and estimated toxicity levels at 20%, 50%, and 80% parasite mortality. Bactericidal activity of target compounds was also tested in two important aquaculture bacteria; Vibrio harveyi and V. anguillarum, while their potential host toxicity was evaluated in gilthead seabream SAF-1 cell line. Synthetic compound bithionate sodium exerted the most potent toxicity against the monogenean, no host cytotoxicity, and a medium and high potency against two bacterial pathogens. In comparison, target natural compounds were approximately 20 (cedrol) or up to 154 times (camphor) less toxic for the monogenean. Rather than completely dismissing natural compounds, we suggest that their application in combination with synthetic drugs, especially if administered in the feed, might be useful in sparicotylosis treatment.
The EBV-Encoded Oncoprotein, LMP1, Recruits and Transforms Fibroblasts via an ERK-MAPK-Dependent Mechanism
Authors: Davis Alexandra M , Rapley Abigail , Dawson Christopher W , Young Lawrence S , Morris Mhairi A
Published: August 2021

Latent membrane protein 1 (LMP1), the major oncoprotein encoded by Epstein–Barr virus (EBV), is expressed at widely variable levels in undifferentiated nasopharyngeal carcinoma (NPC) biopsies, fueling intense debate in the field as to the importance of this oncogenic protein in disease pathogenesis. LMP1-positive NPCs are reportedly more aggressive, and in a similar vein, the presence of cancer-associated fibroblasts (CAFs) surrounding “nests” of tumour cells in NPC serve as indicators of poor prognosis. However, there is currently no evidence linking LMP1 expression and the presence of CAFs in NPC. In this study, we demonstrate the ability of LMP1 to recruit fibroblasts in vitro in an ERK-MAPK-dependent mechanism, along with enhanced viability, invasiveness and transformation to a myofibroblast-like phenotype. Taken together, these findings support a putative role for LMP1 in recruiting CAFs to the tumour microenvironment in NPC, ultimately contributing to metastatic disease.
Alphavirus-Induced Membrane Rearrangements during Replication, Assembly, and Budding
Authors: Elmasri Zeinab , Nasal Benjamin L. , Jose Joyce
Published: August 2021

Alphaviruses are arthropod-borne viruses mainly transmitted by hematophagous insects that cause moderate to fatal disease in humans and other animals. Currently, there are no approved vaccines or antivirals to mitigate alphavirus infections. In this review, we summarize the current knowledge of alphavirus-induced structures and their functions in infected cells. Throughout their lifecycle, alphaviruses induce several structural modifications, including replication spherules, type I and type II cytopathic vacuoles, and filopodial extensions. Type I cytopathic vacuoles are replication-induced structures containing replication spherules that are sites of RNA replication on the endosomal and lysosomal limiting membrane. Type II cytopathic vacuoles are assembly induced structures that originate from the Golgi apparatus. Filopodial extensions are induced at the plasma membrane and are involved in budding and cell-to-cell transport of virions. This review provides an overview of the viral and host factors involved in the biogenesis and function of these virus-induced structures. Understanding virus–host interactions in infected cells will lead to the identification of new targets for antiviral discovery.
An Unusual Occurrence of Erythrocytosis in a Child with Nephrotic Syndrome and Advanced Chronic Kidney Disease
Authors: Acharya Ratna , Upadhyay Kiran
Published: August 2021

Background: Anemia is common in patients with nephrotic syndrome (NS) for various reasons. Furthermore, anemia can occur in patients with chronic kidney disease (CKD) predominantly owing to inappropriately low erythropoietin (EPO) production relative to the degree of anemia. However, erythrocytosis is uncommon in patients with NS and advanced CKD who are not treated with exogenous erythropoietin stimulating agents, and when present, will necessitate exploration of the other etiologies. Case summary: Here, we describe an 8-year-old girl with erythrocytosis in association with NS and advanced CKD. The patient was found to have erythrocytosis during the evaluation for hypertensive urgency. She also had nephrotic range proteinuria without edema. Serum hemoglobin and hematocrit were 17 gm/dL and 51%, respectively, despite hydration. Renal function test showed an estimated glomerular filtration rate of 30 mL/min/1.73 m2. There was mild iron deficiency anemia with serum iron saturation of 18%. Serum EPO level was normal. Urine EPO was not measured. Renal biopsy showed evidence of focal segmental glomerulosclerosis. Genetic testing for NS showed mutations in podocyte genes: NUP93, INF2, KANK1, and ACTN4. Gene sequence analysis of genes associated with erythrocytosis showed no variants in any of these genes. She required chronic dialysis ten months later and, subsequently, a renal transplantation 14 months after the initial presentation. Conclusion: Since the serum EPO level was normal, an increased sensitivity to EPO is the most probable mechanism of erythrocytosis. The unusual association of erythrocytosis in patients with NS and advanced CKD needs to be studied further in larger studies.
Inhibitors of the PI3K/Akt/mTOR Pathway in Prostate Cancer Chemoprevention and Intervention
Authors: Roudsari Nazanin Momeni , Lashgari Naser-Aldin , Momtaz Saeideh , Abaft Shaghayegh , Jamali Fatemeh , Safaiepour Pardis , Narimisa Kiyana , Jackson Gloria , Bishayee Anusha , Rezaei Nima , Abdolghaffari Amir Hossein , Bishayee Anupam
Published: August 2021

The phosphatidylinositol 3-kinase (PI3K)/serine-threonine kinase (Akt)/mammalian target of the rapamycin (mTOR)-signaling pathway has been suggested to have connections with the malignant transformation, growth, proliferation, and metastasis of various cancers and solid tumors. Relevant connections between the PI3K/Akt/mTOR pathway, cell survival, and prostate cancer (PC) provide a great therapeutic target for PC prevention or treatment. Recent studies have focused on small-molecule mTOR inhibitors or their usage in coordination with other therapeutics for PC treatment that are currently undergoing clinical testing. In this study, the function of the PI3K/Akt/mTOR pathway, the consequence of its dysregulation, and the development of mTOR inhibitors, either as an individual substance or in combination with other agents, and their clinical implications are discussed. The rationale for targeting the PI3K/Akt/mTOR pathway, and specifically the application and potential utility of natural agents involved in PC treatment is described. In addition to the small-molecule mTOR inhibitors, there are evidence that several natural agents are able to target the PI3K/Akt/mTOR pathway in prostatic neoplasms. These natural mTOR inhibitors can interfere with the PI3K/Akt/mTOR pathway through multiple mechanisms; however, inhibition of Akt and suppression of mTOR 1 activity are two major therapeutic approaches. Combination therapy improves the efficacy of these inhibitors to either suppress the PC progression or circumvent the resistance by cancer cells.
Preparation of Poly-Lactic-Co-Glycolic Acid Nanoparticles in a Dry Powder Formulation for Pulmonary Antigen Delivery
Authors: Scherließ Regina , Janke Julia
Published: August 2021

One of the key requirements for successful vaccination via the mucosa is particulate antigen uptake. Poly-lactic-co-glycolic acid (PLGA) particles were chosen as well-known model carriers and ovalbumin (OVA) as the model antigen. Aiming at application to the respiratory tract, which allows direct interaction of the formulation with the mucosal immune system, this work focuses on the feasibility of delivering the antigen in a nanoparticulate carrier within a powder capable of pulmonary delivery. Further requirements were adequate antigen encapsulation in order to use the characteristics of the particulate carrier for (tunable) antigen release, and capability of the production process for industrialisation (realisation in industry). For an effective particulate antigen uptake, nanoparticles with a size of around 300 nm were prepared. For this, two production methods for nanoparticles, solvent change precipitation and the double emulsion method, were evaluated with respect to antigen incorporation, transfer to a dry powder formulation, redispersion and antigen release characteristics. A spray drying step was included in the production procedure in order to obtain a respirable powder with an aerodynamic particle size of between 0.5 and 5 μm. The dried products were characterised for particle size, dispersibility and aerodynamic behaviour, as well as for immune response and cytotoxicity in cell culture models. It could be shown that the double emulsion method is suitable to prepare nanoparticles (270 nm) and to incorporate the antigen. By modifying the production method to prepare porous particles, it was possible to obtain an acceptable antigen release while maintaining an antigen load of about 10%. By the choice of polyvinyl alcohol as a stabiliser, nanoparticles could be dried and redispersed without further excipients and the production steps were capable of realisation in industry. Aerodynamic characteristics were good with a mass median aerodynamic diameter of 3.3 µm upon dispersion from a capsule-based inhaler.
Impact of Powder Properties on the Rheological Behavior of Excipients
Authors: Janssen Pauline H. M. , Depaifve Sébastien , Neveu Aurélien , Francqui Filip , Dickhoff Bastiaan H. J.
Published: August 2021

With the emergence of quality by design in the pharmaceutical industry, it becomes imperative to gain a deeper mechanistic understanding of factors impacting the flow of a formulation into tableting dies. Many flow characterization techniques are present, but so far only a few have shown to mimic the die filling process successfully. One of the challenges in mimicking the die filling process is the impact of rheological powder behavior as a result of differences in flow field in the feeding frame. In the current study, the rheological behavior was investigated for a wide range of excipients with a wide range of material properties. A new parameter for rheological behavior was introduced, which is a measure for the change in dynamic cohesive index upon changes in flow field. Particle size distribution was identified as a main contributing factor to the rheological behavior of powders. The presence of fines between larger particles turned out to reduce the rheological index, which the authors explain by improved particle separation at more dynamic flow fields. This study also revealed that obtained insights on rheological behavior can be used to optimize agitator settings in a tableting machine.
Lipid-Polymeric Films: Composition, Production and Applications in Wound Healing and Skin Repair
Authors: Souto Eliana B. , Yoshida Cristiana M. P. , Leonardi Gislaine R. , Cano Amanda , Sanchez-Lopez Elena , Zielinska Aleksandra , Viseras César , Severino Patricia , Silva Classius F. da , Barbosa Raquel de M.
Published: August 2021

The use of lipids in the composition of polymeric-based films for topical administration of bioactive ingredients is a recent research topic; while few products are commercially available, films containing lipids represent a strategic area for the development of new products. Some lipids are usually used in polymeric-based film formulations due to their plasticizing action, with a view to improving the mechanical properties of these films. On the other hand, many lipids have healing, antimicrobial, anti-inflammatory, anti-aging properties, among others, that make them even more interesting for application in the medical-pharmaceutical field. This manuscript discusses the production methods of these films both on a laboratory and at industrial scales, the properties of the developed biopolymers, and their advantages for the development of dermatologic and cosmetic products.
Simulations of Decentration and Tilt of a Supplementary Sulcus-Fixated Intraocular Lens in a Polypseudophakic Combination Using Ray-Tracing Software
Authors: Łabuz Grzegorz , Auffarth Gerd U. , Yan Weijia , Yildirim Timur M. , Khoramnia Ramin
Published: August 2021

This study aimed to assess image quality after the tilt and decentration of supplementary intraocular lenses (IOLs) in a two-lens configuration. One was designed for sulcus fixation with a nominal power range of 1D–10D and was combined with a capsular fixation 20D IOL. The optical performance of a ray-tracing model was tested under IOL misalignment through the area under the modulation transfer function (MTFa) and wave aberrations. Tilting by 10° resulted in a 4% reduction of the MTFa for a 10D IOL as compared to 9% for the 20D lens. The two models demonstrated good tolerance to a 1 mm decentration; as for the 10D sulcus-fixated lens, the MTFa loss was 2%, and 4% for the capsular fixation lens. Coma and astigmatism increased three- and four-fold, respectively, after a 10° tilt compared to the aberration level induced by the 1 mm decentration. Both analyses showed a trend towards a lower MTF impact and fewer optical errors with decreasing nominal power. In conclusion, when misaligned, low-power sulcus-fixated IOLs might retain their good optical quality. An extreme tilt of 10° has a more detrimental effect on the IOL performance than a 1 mm decentration. The proper alignment of a high-power capsular fixation lens is important in achieving a desirable postoperative outcome.
Identification and Fine-Mapping of Quantitative Trait Loci Controlling Plant Height in Central European Winter Triticale (×Triticosecale Wittmack)
Authors: Trini Johannes , Maurer Hans Peter , Neuweiler Jan Eric , Würschum Tobias
Published: August 2021

The quantitatively inherited trait plant height is routinely evaluated in triticale breeding programs as it substantially influences lodging and disease susceptibility, is a main contributor to biomass yield, and is required to improve hybrid seed production by fine-tuning plant height in the female and male parental pools in hybrid breeding programs. In this study, we evaluated a panel of 846 diverse Central European triticale genotypes to dissect the genetic architecture underlying plant height by genome-wide association mapping. This revealed three medium- to large-effect QTL on chromosomes 5A, 4B, and 5R. Genetic and physical fine-mapping of the putative QTL revealed that the QTL on chromosome 5R most likely corresponds to Ddw1 and that the QTL on chromosome 5A is likely to be Rht12. Furthermore, we observed a temporal trend in registered cultivars with a decreasing plant height during the past decades, accompanied by an increasing use of the height-reducing alleles at the identified QTL. In summary, our results shed new light on the genetic control of plant height in triticale and open new avenues for future improvement by breeding.
Transcription Factor-Based Genetic Engineering in Microalgae
Authors: Mochdia Keiichi , Tamaki Shun
Published: August 2021

Sequence-specific DNA-binding transcription factors (TFs) are key components of gene regulatory networks. Advances in high-throughput sequencing have facilitated the rapid acquisition of whole genome assembly and TF repertoires in microalgal species. In this review, we summarize recent advances in gene discovery and functional analyses, especially for transcription factors in microalgal species. Specifically, we provide examples of the genome-scale identification of transcription factors in genome-sequenced microalgal species and showcase their application in the discovery of regulators involved in various cellular functions. Herein, we highlight TF-based genetic engineering as a promising framework for designing microalgal strains for microalgal-based bioproduction.
Effect of Propolis Paste and Mouthwash Formulation on Healing after Teeth Extraction in Periodontal Disease
Authors: Lisbona-González Maria Jesús , Muñoz-Soto Esther , Lisbona-González Cristina , Vallecillo-Rivas Marta , Diaz-Castro Javier , Moreno-Fernandez Jorge
Published: August 2021

This study investigated the antimicrobial effects of a mouthwash containing propolis and the effect of a propolis paste formulation on dental healing after teeth extraction in patients with periodontal disease. In the mouthwash experience, the population comprised 40 patients, which were divided as follows: the control mouthwash, 0.2% chlorhexidine (v/v) mouthwash, 2% (w/v) propolis mouthwash, and propolis + chlorhexidine mouthwash. The study of the propolis paste comprised a population of 60 patients with periodontal disease, and a total of 120 symmetric tooth extractions were performed. Propolis showed antimicrobial activity by itself, and especially with the chlorhexidine association. Three days after surgery in the teeth treated with control paste, only 13.4% had completely healed; however, with propolis paste, in 90% of the periodontal sockets, healing was complete. In addition, a reduction in Streptococci mutans and Lactobacilli cfu was observed with propolis, and especially with the association of chlorhexidine + propolis. Propolis mouthwash reduced bacterial proliferation, especially in association with chlorhexidine. Propolis paste is a viable alternative for socket healing after dental extraction. The knowledge gained from these findings will provide a foundation for similar propolis therapies in order to improve the healing process after dental surgery.
Chitosan-Coated Poly(lactic acid) Nanofibres Loaded with Essential Oils for Wound Healing
Authors: Milanesi Giulia , Vigani Barbara , Rossi Silvia , Sandri Giuseppina , Mele Elisa
Published: August 2021

Chronic skin wounds are characterised by a non-healing process that makes necessary the application of wound dressings on the damaged area to promote and facilitate the recovery of skin’s physiological integrity. The aim of the present work is to develop a bioactive dressing that, once applied on the injured tissue, would exert antibacterial activity and promote adhesion and proliferation of fibroblasts. Nanofibres consisting of poly(lactic acid) (PLA) and essential oils (EOs) were electrospun and coated with a medium molecular weight chitosan (CS). Black pepper essential oil (BP-EO) or limonene (L), well-known for their antibacterial properties, were added to the PLA/acetone solution before electrospinning; phase separation phenomena occurred due to the poor solubility of the EOs in the PLA solution and led to fibres having surface nano-pores. The porous electrospun fibres were coated with CS to produce hydrophilic membranes that were easy to handle, biocompatible, and suited to promote cellular proliferation. The fibrous scaffolds were tested in terms of mechanical resistance, wettability, antibacterial activity, in-vitro cytotoxicity, and ability to promote fibroblasts’ adhesion and proliferation. The results obtained proved that the CS coating improved the hydrophilicity of the fibrous mats, enhanced EO’s antibacterial potential, and promoted cell adhesion and proliferation.
Starch–Mucilage Composite Films: An Inclusive on Physicochemical and Biological Perspective
Authors: Tosif Mansuri M. , Najda Agnieszka , Bains Aarti , Zawiślak Grażyna , Maj Grzegorz , Chawla Prince
Published: August 2021

In recent years, scientists have focused on research to replace petroleum-based components plastics, in an eco-friendly and cost-effective manner, with plant-derived biopolymers offering suitable mechanical properties. Moreover, due to high environmental pollution, global warming, and the foreseen shortage of oil supplies, the quest for the formulation of biobased, non-toxic, biocompatible, and biodegradable polymer films is still emerging. Several biopolymers from varied natural resources such as starch, cellulose, gums, agar, milk, cereal, and legume proteins have been used as eco-friendly packaging materials for the substitute of non-biodegradable petroleum-based plastic-based packaging materials. Among all biopolymers, starch is an edible carbohydrate complex, composed of a linear polymer, amylose, and amylopectin. They have usually been considered as a favorite choice of material for food packaging applications due to their excellent forming ability, low cost, and environmental compatibility. Although the film prepared from bio-polymer materials improves the shelf life of commodities by protecting them against interior and exterior factors, suitable barrier properties are impossible to attain with single polymeric packaging material. Therefore, the properties of edible films can be modified based on the hydrophobic–hydrophilic qualities of biomolecules. Certain chemical modifications of starch have been performed; however, the chemical residues may impart toxicity in the food commodity. Therefore, in such cases, several plant-derived polymeric combinations could be used as an effective binary blend of the polymer to improve the mechanical and barrier properties of packaging film. Recently, scientists have shown their great interest in underutilized plant-derived mucilage to synthesize biodegradable packaging material with desirable properties. Mucilage has a great potential to produce a stable polymeric network that confines starch granules that delay the release of amylose, improving the mechanical property of films. Therefore, the proposed review article is emphasized on the utilization of a blend of source and plant-derived mucilage for the synthesis of biodegradable packaging film. Herein, the synthesis process, characterization, mechanical properties, functional properties, and application of starch and mucilage-based film are discussed in detail.
Palm Oil Decanter Cake Wastes as Alternative Nutrient Sources and Biomass Support Particles for Production of Fungal Whole-Cell Lipase and Application as Low-Cost Biocatalyst for Biodiesel Production
Authors: H-Kittikun Aran , Cheirsilp Benjamas , Sohsomboon Natthapat , Binmarn Darun , Pathom-aree Wasu , Srinuanpan Sirasit
Published: August 2021

This is the first report on the possible use of decanter cake waste (DCW) from palm oil industry as alternative nutrient sources and biomass support particles for whole-cell lipase production under solid-state fermentation (SSF) by newly isolated fungal Aspergillus sp. MS15 and their application as a low-cost and environment-friendly biocatalyst for biodiesel production. The results found that DCW supplemented with 0.1% K2HPO4, 0.05% MgSO4·7H2O, 1% peptone and 2% urea and pH adjusted to 6.0 was optimal for whole-cell lipase production. The optimal moisture content and fermentation temperature was 60% and 37.5 °C, respectively. Environmentally friendly biodiesel production, through either esterification or transesterification using whole-cell lipase immobilized on DCW as a biocatalyst, was optimized. The optimal reaction temperature for both reactions was 37 °C. The whole-cell lipase effectively esterified oleic acid into >95% biodiesel yield through esterification under optimal water activity at 0.71 and an optimal methanol to oleic acid molar ratio of 2:1, and also effectively transesterified palm oil under optimal water activity at 0.81 and an optimal methanol to oil molar ratio of 3:1. The fuel properties of produced biodiesel are close to the international biodiesel standards. These results have shown the circular utilization of palm oil mill waste for the low-cost production of an effective biocatalyst, and may contribute greatly to the sustainability of renewable bioenergy production.
The Biblical Image of the Providential Ruler in the Protestant Propaganda on the Eve of the French Wars of Religion
Authors: Sălăvăstru Andrei Constantin
Published: August 2021

French Protestantism has remained famous in the history of political thought mostly for its theories regarding popular sovereignty and the right of the people to resist and replace a tyrannical ruler. However, before the civil wars pushed them on this revolutionary path, French Protestants stressed the duty of obedience even in the face of manifest tyranny. The reasons for this were ideological, due to the significance placed on St. Paul’s assertion that all political power was divinely ordained, but also pragmatic, as Calvin and his followers were acutely aware of the danger of antagonizing the secular authorities. More importantly, they were fervently hoping for the conversion of France to the Reformation and, in their mind, the surest way such a process could take place was through the conversion of the king and the royal family. Therefore, Protestant propaganda of that time constantly urged the most important French royals to convert to the Reformation, and, for this purpose, they deployed a language full of references to the pious Biblical rulers who led their people towards the true faith—whom the addressees of these propaganda texts were advised to emulate, lest they incur God’s wrath. This paper aims to analyze the occurrences and the role of these references in the Protestants’ dialogue with the French monarchy.
The Role of Catholicism in Shaping a Culture of Sustainable Consumption
Authors: Sadowski Ryszard F.
Published: August 2021

The paper presents the potential of religions, in particular Christianity, in shaping a culture of sustainable consumption (a culture of moderation). It focuses on the contribution of the Catholic tradition, which is, to some extent, complemented by statements representing other Christian denominations. Based on an analysis of relevant sources, it identifies risks arising from the prevalence of a consumer culture, which results in the primacy of “having” over “being” and reduces man to a Homo consumens. Moreover, the widespread culture of consumerism is associated with a so-called throwaway culture dominated by a disposable mentality making use of things and people. It also points to one of the key elements of consumer culture (a culture of overuse) in the form of so-called neophilia, resulting in the weakening of interpersonal relationships, the atomization of societies, and depletion of the Earth’s resources, combined with reckless wastefulness. An analysis of consumer culture leads to the identification of elements that underpin a culture of moderation, being an antidote to dangers arising from the prevalence of consumer culture. The most important elements shaping a model of culture that is man- and environment-friendly include a new model of progress, a mentality of communion, and a new lifestyle. It is a model of progress, which is not limited merely to economics, but takes into account the need for integral human development and appreciates the spiritual and cultural sphere of life. In order to build a culture of moderation, a communion mentality is needed to restore interpersonal relationships and make man aware of his relation to the whole community of life. Another important element of a culture of moderation is a new lifestyle which takes into account all human needs, respects the potential of the natural environment, and is characterized by intra-generational and inter-generational responsibility. There is much to suggest that Christianity has the potential to make a significant contribution to reducing the culture of overuse and promoting a culture of moderation. The influence of Catholic thought on shaping a culture of sustainable consumption is illustrated by the introduction of restrictions on Sunday shopping in Poland in 2018. This case study shows the importance of religious arguments in introducing legal changes and their impact on promoting a culture of moderation.
Concepts and Methods in the Study of the Qur’ān
Authors: Dye Guillaume
Published: August 2021

This paper addresses methodological issues in Qur’anic studies. At first, it intends to explain, through historiographical analysis, why methods proved fruitful in biblical and New Testament studies, such as form criticism and redaction criticism, have been disregarded in Qur’anic studies; secondly, it vindicates the application of such methods to the Qur’anic corpus; thirdly, it tries to exemplify the relevance of redaction criticism through examples. Two main issues are then discussed: the best way to account for the “synoptic problem” (the presence, in the Qur’ān, of variant parallel narratives), through an examination of some aspects of the Adam-Iblīs narratives (more precisely the composition of Q 2:30–38 and the nature of the relations between Q 38:71–85 and Q 15:26–43); and the beginning of Q 55. Two main conclusions are reached: first, the later versions of a parallel story are, in the examples discussed here, rewritings of earlier stories (namely, re-compositions based on a written version); second, sura 55 features the intervention of different authors, with two different profiles.
What and How Hybrid Forms of Christian Social Enterprises Are Created and Sustained in Cambodia? A Critical Realist Institutional Logics Perspective
Authors: Kimura Rikio
Published: August 2021

On top of the well-known dilemma of social enterprises as hybrid organizations, the form in which they struggle to balance business viability and the fulfillment of social missions, faith-based social enterprises have an added dimension: their spirituality manifested as organizational culture and practices based on their spiritual values and mission to spread their faith. By employing critical realist institutional logics and an identity-based and biographical approach to social entrepreneurship, this study identifies a typology of different hybrid forms of Christian social enterprises in Cambodia and the tensions associated with them. Moreover, this study explores how and why their social entrepreneurs have created and sustained such forms. I analyzed the qualitative data of 12 Christian social enterprises mainly from interviews with their entrepreneurs. Broadly speaking, the analysis revealed that the hybrid forms of these enterprises depend on the entrepreneurs’ agency, which is influenced by their biographies and contexts. Particularly, in addition to the entrepreneurs’ possession and enactment of multiple identities, boards of directors (as part of the context) and their accountability pressures are crucial for Christian social enterprises to achieve the triple bottom line of business viability, social missions, and spiritual outcomes.
Acute Facial Edema in a Patient with Systemic Lupus Erythematosus
Authors: Amano Shiho , Ohta Ryuichi , Sano Chiaki
Published: August 2021

Allergies have been found to be associated with systemic lupus erythematosus (SLE). However, few reports have described angioedema occurring in elderly men with systemic lupus erythematosus. Herein, we report the case of an 85-year-old man who presented with angioedema with eosinophilia. The patient was initially thought to have a drug-induced allergy. The differentiation between allergic reactions caused by drugs and those caused by eosinophilia with SLE can be challenging. The effect of the withdrawal of the suspected culprit drug and allergic dermal findings can be key to differentiating the two conditions. SLE is prevalent among younger generations; hence, active immunity can induce various symptoms, including eosinophilia, which causes angioedema. Even older people with SLE can have a strong immune reaction, resulting in angioedema with eosinophilia. In cases of localized facial edema in elderly patients with SLE, it is critical to consider angioedema caused by eosinophilia as a differential diagnosis.
Time-Series of Cloud-Free Sentinel-2 NDVI Data Used in Mapping the Onset of Growth of Central Spitsbergen, Svalbard
Authors: Karlsen Stein Rune , Stendardi Laura , Tømmervik Hans , Nilsen Lennart , Arntzen Ingar , Cooper Elisabeth J.
Published: August 2021

The Arctic is a region that is expected to experience a high increase in temperature. Changes in the timing of phenological phases, such as the onset of growth (as observed by remote sensing), is a sensitive bio-indicator of climate change. In this paper, the study area was the central part of Spitsbergen, Svalbard, located between 77.28°N and 78.44°N. The goals of this study were: (1) to prepare, analyze and present a cloud-free time-series of daily Sentinel-2 NDVI datasets for the 2016 to 2019 seasons, and (2) to demonstrate the use of the dataset in mapping the onset of growth. Due to a short and intense period with greening-up and frequent cloud cover, all the cloud-free Sentinel-2 data were used. The onset of growth was then mapped by a NDVI threshold method, which showed significant correlation (r2 = 0.47, n = 38, p < 0.0001) with ground-based phenocam observation of the onset of growth in seven vegetation types. However, large bias was found between the Sentinel-2 NDVI-based mapped onset of growth and the phenocam-based onset of growth in a moss tundra, which indicates that the data in these vegetation types must be interpreted with care. In 2018, the onset of growth was about 10 days earlier compared to 2017.
Impact of GPS/BDS Satellite Attitude Quaternions on Precise Point Positioning with Ambiguity Resolution
Authors: Yang Songfeng , Zhang Qiyuan , Zhang Xi , Liu Donglie
Published: August 2021

Precise point positioning with ambiguity resolution (PPP-AR) based on multiple global navigation satellite system (multi-GNSS) constellations is an important high-precision positioning tool. However, some unmodeled satellite and receiver biases (such as errors in satellite attitude) make it difficult to fix carrier-phase ambiguities. In order to fix ambiguities of eclipsing satellites, accurate integer clock and satellite attitude products (i.e., attitude quaternion) have been provided by the International GNSS Service (IGS). Nevertheless, the quality of these products and their positioning performance in multi-GNSS PPP-AR have not been investigated yet. Using the PRIDE PPP-AR II software associated with the corresponding rapid satellite orbit, integer clock and attitude quaternion products of Wuhan University (WUM), we carried out GPS/BDS PPP-AR using 30 days of data in an eclipsing season of 2020. We found that about 75% of GPS, 60% of BDS-2 and 57% of BDS-3 narrow-lane ambiguity residuals after integer clock corrections fall within ±0.1 cycles in the case of using nominal attitudes. However, when using attitude quaternions, these percentages will rise to 80% for GPS, 70% for BDS-2 and 60% for BDS-3. GPS/BDS daily kinematic PPP-AR after integer clock and nominal attitude corrections can usually achieve a positioning precision of about 10, 10 and 30 mm for the east, north and up components, respectively. In contrast, the counterparts are 8, 8 and 20 mm when using attitude quaternions. Compared with the case of using attitude quaternions only at the network end for the integer clock estimation, using attitude quaternions only at the user end shows a pronounced improvement of 15% in the east component and less than 10% in the north and up components. Therefore, we suggest PPP users apply integer clock and satellite attitude quaternion products to realize more efficient ambiguity fixing, especially in satellite eclipsing seasons.