bluuebunny/crossref_metadata_2025 huggingface dataset:
A fatal pediatric infection with a C1-like subgenogroup enterovirus A71: case study and enterovirus A71 epidemiology in Finland
Authors: Nieminen Tea , Jääskeläinen Anne J. , Lindh Erika , Blomqvist Soile , Savolainen-Kopra Carita
Published: April 2024

Enterovirus A71 (EV-A71) is among the most neuropathogenic non-polio enterovirus types and, in rare instances, can lead to severe or even fatal outcomes, particularly in children under 5 years of age. This case study presents clinical and microbiological findings from the initial documented severe pediatric EV-A71 case in Finland, identified in May 2019. The near-complete genome sequence confirms that the EV-A71 strain belongs to the newly identified recombinant C1-like EV-A71 genetic lineage, which emerged in 2015 and has since been circulating in Europe, causing severe cases among children in various European countries. Enhanced environmental surveillance revealed widespread circulation of EV-A71 in Finland in 2019. However, the overall number of EV clinical cases remained lower than in previous years.
Verbal expressions describing itch quality in atopic dermatitis and urticaria: an online questionnaire survey in Japan
Authors: Ohya Yukihiro , Ebata Toshiya , Ohshima Yusei , Andoh Tsugunobu , Tominaga Mitsutoshi , Kataoka Yoko , Fukui Yoshinori , Ebihara Nobuyuki , Hasegawa Shunji , Kobayashi Shigetoshi , Morisawa Yutaka , Inoue Norihiro , Narita Masami , Kaneko Sakae , Igawa Ken , Nakahara Takeshi , Ishiuji Yozo , Okada Takaharu , Fujii Masanori , Kawasaki Hiroshi , Irie Hiroyuki , Shiratori-Hayashi Miho , Murota Hiroyuki
Published: April 2024

Background: The nature of itch sensation varies depending upon the patient and the disease. However, few studies have focused on verbal expressions describing itch of atopic dermatitis (AD) in quality.

Objectives: To investigate itch quality in patients with AD compared with that of urticaria.

Methods: We conducted an online questionnaire survey describing itch experiences in June 2021. Participants were Japanese patients who had visited hospitals for their consultations and treatments of AD or urticaria in the last 6 months, and 295 and 290 responses, respectively, to questions using 12 terms describing itch quality were analyzed.

Results: The most suitable expression describing intense itch that patients could not help scratching differed between the diseases, where most AD patients selected “muzumuzu” (a mimetic word for creepy–crawly itch) (27%) or “painful itch” (20%), and most urticaria patients selected “muzumuzu” (24%) or “itch like mosquito bites” (22%). The most suitable expressions describing itch that would make patients happiest if improved was “painful itch” (27%) in AD patients, significantly higher than urticaria patients (19%). More AD patients (55%) responded that they sometimes felt itch even after the skin symptoms had subsided than urticaria patients (41%). The most suitable expression of remnant itch selected was “muzumuzu” for AD (58/161 patients, 36%) and urticaria (29/120 patients, 24%).

Conclusion: The quality of itch sensations can be classified not only between diseases but also during the clinical course of each disease. Significant expressions that patients with AD use to describe itch sensations could promote more appropriate treatment for itch.
Comparative Measurement of Horizontal Penetrometry with a Focus on the Degree of Soil Compaction in Real Work Conditions
Authors: Mojžiš Marek , Kosiba Ján , Jobbágy Ján
Published: April 2024

Potential soil production is closely related to the physical and mechanical properties. The aim of this paper was to evaluate the effect of different levels of soil compaction created by tractor chassis. The total area of the experimental plot was 13.22 ha. Up until 2019, a conventional tillage system had been used. The measurements were carried out with an innovative measuring device that allows for the continuous measurement of the horizontal penetrometry for comparative measurements while driving, which was designed at the Slovak University of Agriculture in Nitra. The measuring device measured the soil resistance in the tire track (On-track) and out of track (Off-track) as well as in three (50 s) sequences within one tractor pass. Three lines were chosen, where in each a pair of combinations was made. The results were subjected, in addition to graphical evaluation, to single factor ANOVA analysis. When comparing individual passes (PH1 to PH6), the statistical analysis showed that the results of the horizontal resistance measurements proved to be statistically significant (p < 0.05) with respect to the weight, number of passes, and tire underinflation. The highest compaction was caused by the first pass, while the higher weight of the tractor during the next pass had a smaller effect. Underinflating the tires ensured a reduction in compaction. Reducing the tractor tire pressure to 0.15 MPa resulted in a reduction in soil compaction of up to 16%.
Numerical Analysis of Broadband Noise Generated by an Airfoil with Spanwise-Varying Leading Edges
Authors: Wang Lei , Liu Xiaomin , Tian Chenye , Li Dian
Published: April 2024

Here, the single-target parameterization of alternatives to leading-edge noise is carried out using analytical models based on the Wiener–Hopf technique. Four leading-edge serration profiles with different frequencies, amplitudes, and phases are implemented to aid the understanding of sound suppression mechanisms. The effects of the serrated shape factor, wavelength, and amplitude are analyzed at tip-to-root ratios of 0.5, 1, and 2, respectively. An effective double-wavelength sinusoidal serration design can substantially reduce the noise emissions of 5.2 dB at h¯ = 2. Additionally, compared to single-wavelength serrations, an additional 1.47 dB noise reduction effect can be obtained by double-wavelength serrations under the appropriate design parameters. The surface pressure and phase distribution of different spanwise-varying leading edges indicate that the phase interference effect affected by source-radiated noise reduction is enhanced by this serration at the hills for serrations with a small curvature, and noise emission in the low-frequency band is more effectively suppressed. The sharper the serration is, the more conducive it is to a reduction in high-frequency noise. Nevertheless, the effectiveness of serrations is usually partially limited by the non-negligible trailing-edge self-noise. The sound source intensity of the root is decreased by the ogee-shaped serrations with a large curvature transition. A secondary noise reduction mechanism with a local source cut-off effect caused by nonlinearity is demonstrated.
Optimization of a BEGe Detector Setup for Testing Quantum Foundations in the Underground LNGS Laboratory
Authors: Piscicchia Kristian , Clozza Alberto , Sirghi Diana , Bazzi Massimiliano , Bortolotti Nicola , Bragadireanu Mario , Cargnelli Michael , De Paolis Luca , Del Grande Raffaele , Guaraldo Carlo , Iliescu Mihail , Laubenstein Matthias , Manti Simone , Marton Johann , Miliucci Marco , Napolitano Fabrizio , Porcelli Alessio , Scordo Alessandro , Sgaramella Francesco , Sirghi Florin , Tomassini Sandro , Doce Oton , Zmeskal Johann , Curceanu Catalina
Published: April 2024

In this work, we report on tests performed with an experimental apparatus prototype based on a broad-energy germanium detector aimed at investigating topical, foundational issues in quantum mechanics: i.e., possible violations of the spin-statistics connection and models of dynamical wave function collapse. Our recent phenomenological analyses demonstrated the importance of pushing the research of new physics signal, predicted in these fields, to an energy range below 10 keV. We describe the development of the dedicated data acquisition system and of the pulse shape discrimination algorithm, which have already allowed us to get a factor two improvement in the lower energy threshold. Future plans are discussed to further improve the lower energy threshold to the level of a few keV.
Corrections to the Bekenstein–Hawking Entropy of the HNUTKN Black Hole Due to Lorentz-Breaking Fermionic Einstein–Aether Theory
Authors: Tan Xia , Wang Cong , Yang Shu-Zheng
Published: April 2024

A hot NUT–Kerr–Newman black hole is a general stationary axisymmetric black hole. In this black hole spacetime, the dynamical equations of fermions at the horizon are modified by considering Lorentz breaking. The corrections to the Hawking temperature and Bekenstein–Hawking entropy at the horizon of the black hole are studied in depth. Based on the semiclassical theory correction, the Bekenstein–Hawking entropy of this black hole is quantum-corrected by considering the perturbation effect of the Planck constant ℏ. The latter part of this paper presents a detailed discussion of the obtained results and their physical implications.
Impact of Short-Circuit Ratio on Control Parameter Settings of DFIG Wind Turbines
Authors: Pedra Joaquín , Sainz Luis , Monjo Lluís
Published: April 2024

This work deals with doubly fed induction generator (DFIG) modeling and stability when connected to weak AC grids. A detailed state-space model that includes the phase-locked loop (PLL) is developed. This work aims to determine the influence of the network’s strength on DFIG stability through the short-circuit ratio (SCR). The critical values of the proportional control parameters of the grid-side and rotor-side converters (RSC and GSC), as well as PLL, which make the system unstable, are calculated for different SCR values. Finally, PSCAD/EMTDC dynamic simulations are used to validate the critical control parameters obtained by studying the eigenvalues of the DFIG state-space model regarding system stability.
Analysis of Anthropogenic Waste Heat Emission from an Academic Data Center
Authors: Ding Weijian , Ebrahimi Behzad , Kim Byoung-Do , Devenport Connie L. , Childress Amy E.
Published: April 2024

The rapid growth in computing and data transmission has significant energy and environmental implications. While there is considerable interest in waste heat emission and reuse in commercial data centers, opportunities in academic data centers remain largely unexplored. In this study, real-time onsite waste heat data were collected from a typical academic data center and an analysis framework was developed to determine the quality and quantity of waste heat that can be contained for reuse. In the absence of a comprehensive computer room monitoring system, real-time thermal data were collected from the data center using two arrays of thermometers and thermo-anemometers in the server room. Additionally, a computational fluid dynamics model was used to simulate temperature distribution and identify “hot spots” in the server room. By simulating modification of the server room with a hot air containment system, the return air temperature increased from 23 to 46 °C and the annual waste heat energy increased from 377 to 2004 MWh. Our study emphasizes the importance of containing waste heat so that it can be available for reuse, and also, that reusing the waste heat has value in not releasing it to the environment.
Predator–Prey Interaction with Fear Effects: Stability, Bifurcation and Two-Parameter Analysis Incorporating Complex and Fractal Behavior
Authors: Din Qamar , Naseem Raja Atif , Shabbir Muhammad Sajjad
Published: April 2024

This study investigates the dynamics of predator–prey interactions with non-overlapping generations under the influence of fear effects, a crucial factor in ecological research. We propose a novel discrete-time model that addresses limitations of previous models by explicitly incorporating fear. Our primary question is: How does fear influence the stability of predator–prey populations and the potential for chaotic dynamics? We analyze the model to identify biologically relevant equilibria (fixed points) and determine the conditions for their stability. Bifurcation analysis reveals how changes in fear levels and predation rates can lead to population crashes (transcritical bifurcation) and complex population fluctuations (period-doubling and Neimark–Sacker bifurcations). Furthermore, we explore the potential for controlling chaotic behavior using established methods. Finally, two-parameter analysis employing Lyapunov exponents, spectrum, and Kaplan–Yorke dimension quantifies the chaotic dynamics of the proposed system across a range of fear and predation levels. Numerical simulations support the theoretical findings. This study offers valuable insights into the impact of fear on predator–prey dynamics and paves the way for further exploration of chaos control in ecological models.
Effects of Fat and Carnitine on the Expression of Carnitine Acetyltransferase and Enoyl-CoA Hydratase Short-Chain 1 in the Liver of Juvenile GIFT (Oreochromis niloticus)
Authors: Guo Ruijie , Huang Kai , Yu Kai , Li Jinghua , Huang Jiao , Wang Dandan , Li Yuda
Published: April 2024

Carnitine acetyltransferase (CAT) and Enoyl-CoA hydratase short-chain 1 (ECHS1) are considered key enzymes that regulate the β-oxidation of fatty acids. However, very few studies have investigated their full length and expression in genetically improved farmed tilapia (GIFT, Oreochromis niloticus), an important aquaculture species in China. Here, we cloned CAT and ECHS1 full-length cDNA via the rapid amplification of cDNA ends, and the expressions of CAT and ECHS1 in the liver of juvenile GIFT were detected in different fat and carnitine diets, as were the changes in the lipometabolic enzymes and serum biochemical indexes of juvenile GIFT in diets with different fat and carnitine levels. CAT cDNA possesses an open reading frame (ORF) of 2167 bp and encodes 461 amino acids, and the ECHS1 cDNA sequence is 1354 bp in full length, the ORF of which encodes a peptide of 391 amino acids. We found that juvenile GIFT had higher lipometabolic enzyme activity and lower blood CHOL, TG, HDL-C, and LDL-C contents when the dietary fat level was 2% or 6% and when the carnitine level was 500 mg/kg. We also found that the expression of ECHS1 and CAT genes in the liver of juvenile GIFT can be promoted by a 500 mg/kg carnitine level and 6% fat level feeding. These results suggested that CAT and ECHS1 may participate in regulating lipid metabolism, and when 2% or 6% fat and 500 mg/kg carnitine are added to the feed, it is the most beneficial to the liver and lipid metabolism of juvenile GIFT. Our results may provide a theoretical basis for GIFT feeding and treating fatty liver disease.
Association between the GLP1R A316T Mutation and Adolescent Idiopathic Scoliosis in French Canadian and Italian Cohorts
Authors: Normand Émilie , Franco Anita , Parent Stefan , Lombardi Giovanni , Brayda-Bruno Marco , Colombini Alessandra , Moreau Alain , Marcil Valérie
Published: April 2024

Studies have revealed anthropometric discrepancies in girls with adolescent idiopathic scoliosis (AIS) compared to non-scoliotic subjects, such as a higher stature, lower weight, and lower body mass index. While the causes are still unknown, it was proposed that metabolic hormones could play a role in AIS pathophysiology. Our objectives were to evaluate the association of GLP1R A316T polymorphism in AIS susceptibility and to study its relationship with disease severity and progression. We performed a retrospective case–control association study with controls and AIS patients from an Italian and French Canadian cohort. The GLP1R rs10305492 polymorphism was genotyped in 1025 subjects (313 non-scoliotic controls and 712 AIS patients) using a validated TaqMan allelic discrimination assay. Associations were evaluated by odds ratio and 95% confidence intervals. In the AIS group, there was a higher frequency of the variant genotype A/G (4.2% vs. 1.3%, OR = 3.40, p = 0.016) and allele A (2.1% vs. 0.6%, OR = 3.35, p = 0.017) than controls. When the AIS group was stratified for severity (≤40° vs. >40°), progression of the disease (progressor vs. non-progressor), curve type, or body mass index, there was no statistically significant difference in the distribution of the polymorphism. Our results support that the GLP1R A316T polymorphism is associated with a higher risk of developing AIS, but without being associated with disease severity and progression.
Tumor-Extrinsic Axl Expression Shapes an Inflammatory Microenvironment Independent of Tumor Cell Promoting Axl Signaling in Hepatocellular Carcinoma
Authors: Breitenecker Kristina , Heiden Denise , Demmer Tobias , Weber Gerhard , Primorac Ana-Maria , Hedrich Viola , Ortmayr Gregor , Gruenberger Thomas , Starlinger Patrick , Herndler-Brandstetter Dietmar , Barozzi Iros , Mikulits Wolfgang
Published: April 2024

The activation of the receptor tyrosine kinase Axl by Gas6 is a major driver of tumorigenesis. Despite recent insights, tumor cell-intrinsic and -extrinsic Axl functions are poorly understood in hepatocellular carcinoma (HCC). Thus, we analyzed the cell-specific aspects of Axl in liver cancer cells and in the tumor microenvironment. We show that tumor-intrinsic Axl expression decreased the survival of mice and elevated the number of pulmonary metastases in a model of resection-based tumor recurrence. Axl expression increased the invasion of hepatospheres by the activation of Akt signaling and a partial epithelial-to-mesenchymal transition (EMT). However, the liver tumor burden of Axl+/+ mice induced by diethylnitrosamine plus carbon tetrachloride was reduced compared to systemic Axl−/− mice. Tumors of Axl+/+ mice were highly infiltrated with cytotoxic cells, suggesting a key immune-modulatory role of Axl. Interestingly, hepatocyte-specific Axl deficiency did not alter T cell infiltration, indicating that these changes are independent of tumor cell-intrinsic Axl. In this context, we observed an upregulation of multiple chemokines in Axl+/+ compared to Axl−/− tumors, correlating with HCC patient data. In line with this, Axl is associated with a cytotoxic immune signature in HCC patients. Together these data show that tumor-intrinsic Axl expression fosters progression, while tumor-extrinsic Axl expression shapes an inflammatory microenvironment.
A Biodegradable Tissue Adhesive for Post-Extraction Alveolar Bone Regeneration under Ongoing Anticoagulation—A Microstructural Volumetric Analysis in a Rodent Model
Authors: Heitzer Marius , Winnand Philipp , Ooms Mark , Magnuska Zuzanna , Kiessling Fabian , Buhl Eva Miriam , Hölzle Frank , Modabber Ali
Published: April 2024

In addition to post-extraction bleeding, pronounced alveolar bone resorption is a very common complication after tooth extraction in patients undergoing anticoagulation therapy. The novel, biodegenerative, polyurethane adhesive VIVO has shown a positive effect on soft tissue regeneration and hemostasis. However, the regenerative potential of VIVO in terms of bone regeneration has not yet been explored. The present rodent study compared the post-extraction bone healing of a collagen sponge (COSP) and VIVO in the context of ongoing anticoagulation therapy. According to a split-mouth design, a total of 178 extraction sockets were generated under rivaroxaban treatment, of which 89 extraction sockets were treated with VIVO and 89 with COSP. Post-extraction bone analysis was conducted via in vivo micro-computed tomography (µCT), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) after 5, 10, and 90 days. During the observation time of 90 days, µCT analysis revealed that VIVO and COSP led to significant increases in both bone volume and bone density (p ≤ 0.001). SEM images of the extraction sockets treated with either VIVO or COSP showed bone regeneration in the form of lamellar bone mass. Ratios of Ca/C and Ca/P observed via EDX indicated newly formed bone matrixes in both treatments after 90 days. There were no statistical differences between treatment with VIVO or COSP. The hemostatic agents VIVO and COSP were both able to prevent pronounced bone loss, and both demonstrated a strong positive influence on the bone regeneration of the alveolar ridge post-extraction.
Green Approach for Synthesis of Silver Nanoparticles with Antimicrobial and Antioxidant Properties from Grapevine Waste Extracts
Authors: Baroi Anda Maria , Fierascu Irina , Ghizdareanu Andra-Ionela , Trica Bogdan , Fistos Toma , Matei (Brazdis) Roxana Ioana , Fierascu Radu Claudiu , Firinca Cristina , Sardarescu Ionela Daniela , Avramescu Sorin Marius
Published: April 2024

The present work aims to study the possibilities of developing silver nanoparticles using natural extracts of grape pomace wastes originating from the native variety of Fetească Neagră 6 Șt. This study focused on investigating the influence of grape pomace extract obtained by two different extraction methods (classical temperature extraction and microwave-assisted extraction) in the phytosynthesis process of metal nanoparticles. The total phenolic content of the extracts was assessed using the spectrophotometric method with the Folin–Ciocâlteu reagent, while the identification and quantification of specific components were conducted through high-performance liquid chromatography with a diode array detector (HPLC-DAD). The obtained nanoparticles were characterized by UV–Vis absorption spectroscopy, X-ray diffraction (XRD), and transmission electron microscopy (TEM), along with assessing their antioxidant and antimicrobial properties against Gram-positive bacteria. The data collected from the experiments indicated that the nanoparticles were formed in a relatively short period of time (96 h) and, for the experimental variant involving the use of a 1:1 ratio (v/v, grape pomace extract: silver nitrate) for the nanoparticle phytosynthesis, the smallest crystallite sizes (from X-ray diffraction—4.58 nm and 5.14 nm) as well as spherical or semispherical nanoparticles with the lowest average diameters were obtained (19.99–23 nm, from TEM analysis). The phytosynthesis process was shown to enhance the antioxidant properties (determined using the DPPH assay) and the antimicrobial potential (tested against Gram-positive strains) of the nanoparticles, as evidenced by comparing their properties with those of the parent extracts; at the same time, the nanoparticles exhibited a selectivity in action, being active against the Staphylococcus aureus strain while presenting no antimicrobial potential against the Enterococcus faecalis strain.
Methodology and Characterization of a 3D Bone Organoid Model Derived from Murine Cells
Authors: Fuller Jaymes , Lefferts Katherine Sares , Shah Pooja , Cottrell Jessica A.
Published: April 2024

Here, we report on the development of a cost-effective, well-characterized three-dimensional (3D) model of bone homeostasis derived from commonly available stocks of immortalized murine cell lines and laboratory reagents. This 3D murine-cell-derived bone organoid model (3D-mcBOM) is adaptable to a range of contexts and can be used in conjunction with surrogates of osteoblast and osteoclast function to study cellular and molecular mechanisms that affect bone homeostasis in vitro or to augment in vivo models of physiology or disease. The 3D-mcBOM was established using a pre-osteoblast murine cell line, which was seeded into a hydrogel extracellular matrix (ECM) and differentiated into functional osteoblasts (OBs). The OBs mineralized the hydrogel ECM, leading to the deposition and consolidation of hydroxyapatite into bone-like organoids. Fourier-transform infrared (FTIR) spectroscopy confirmed that the mineralized matrix formed in the 3D-mcBOM was bone. The histological staining of 3D-mcBOM samples indicated a consistent rate of ECM mineralization. Type I collagen C-telopeptide (CTX1) analysis was used to evaluate the dynamics of OC differentiation and activity. Reliable 3D models of bone formation and homeostasis align with current ethical trends to reduce the use of animal models. This functional model of bone homeostasis provides a cost-effective model system using immortalized cell lines and easily procured supplemental compounds, which can be assessed by measuring surrogates of OB and OC function to study the effects of various stimuli in future experimental evaluations of bone homeostasis.
Unveiling Therapeutic Potential: Targeting Fusobacterium nucleatum’s Lipopolysaccharide Biosynthesis for Endodontic Infections—An In Silico Screening Study
Authors: Boreak Nezar , Alrajab Ethar Awad , Nahari Rayan Ali , Najmi Loay Ebrahim , Masmali Muhannad Ali , Ghawi Atiah Abdulrahman , Al Moaleem Mohammed M. , Alhazmi Majed Yahya , Maqbul Abdulrahman Abdullah
Published: April 2024

Complex microbial communities have been reported to be involved in endodontic infections. The microorganisms invade the dental pulp leading to pulpitis and initiating pulp inflammation. Fusobacterium nucleatum is a dominant bacterium implicated in both primary and secondary endodontic infections. Drugs targeting the molecular machinery of F. nucleatum will minimize pulp infection. LpxA and LpxD are early acyltransferases involved in the formation of lipid A, a major component of bacterial membranes. The identification of leads which exhibit preference towards successive enzymes in a single pathway can also prevent the development of bacterial resistance. A stringent screening strategy utilizing physicochemical and pharmacokinetic parameters along with a virtual screening approach identified two compounds, Lomefloxacin and Enoxacin, with good binding affinity towards the early acyltransferases LpxA and LpxD. Lomefloxacin and Enoxacin, members of the fluoroquinolone antibiotic class, exhibit wide-ranging activity against diverse bacterial strains. Nevertheless, their effectiveness in the context of endodontic treatment requires further investigation. This study explored the potential of Lomefloxacin and Enoxacin to manage endodontic infections via computational analysis. Moreover, the compounds identified herein serve as a foundation for devising novel combinatorial libraries with enhanced efficacy for endodontic therapeutic strategies.
Dielectric Response of Different Alcohols in Water-Rich Binary Mixtures from THz Ellipsometry
Authors: Mazaheri Zahra , Papari Gian Paolo , Andreone Antonello
Published: April 2024

We report a study on the hydrogen bonding mechanisms of three aliphatic alcohols (2-propanol, methanol, and ethanol) and one diol (ethylene glycol) in water solution using a time-domain ellipsometer in the THz region. The dielectric response of the pure liquids is nicely modeled by the generalized Debye–Lorentz equation. For binary mixtures, we analyze the data using a modified effective Debye model, which considers H-bond rupture and reformation dynamics and the motion of the alkyl chains and of the OH groups. We focus on the properties of the water-rich region, finding anomalous behavior in the absorption properties at very low solute molar concentrations. These results, first observed in the THz region, are in line with previous findings from different experiments and can be explained by taking into account the amphiphilic nature of the alcohol molecules.
Apoptotic Effect of Isoimpertorin via Inhibition of c-Myc and SIRT1 Signaling Axis
Authors: Ko Hwan-Joo , Park Su-Yeon , Sim Deok Yong , Kim Sung-Hoon , Hur Soyoung , Lee Jang-Hoon , Kim Youngchul
Published: April 2024

Though Isoimperatorin from Angelicae dahuricae is known to have antiviral, antidiabetic, anti-inflammatory and antitumor effects, its underlying antitumor mechanism remains elusive so far. Hence, the apoptotic mechanism of Isoimperatorin was explored in hepatocellular carcinomas (HCCs). In this study, Isoimperatorin inhibited the viability of Huh7 and Hep3B HCCs and increased the subG1 apoptotic portion and also abrogated the expression of pro-poly-ADP ribose polymerase (pro-PARP) and pro-caspase 3 in Huh7 and Hep3B cells. Also, Isoimperatorin abrogated the expression of cyclin D1, cyclin E1, CDK2, CDK4, CDK6 and increased p21 as G1 phase arrest-related proteins in Huh7 and Hep3B cells. Interestingly, Isoimperatorin reduced the expression and binding of c-Myc and Sirtuin 1 (SIRT1) by Immunoprecipitation (IP), with a binding score of 0.884 in Huh7 cells. Furthermore, Isoimperatorin suppressed the overexpression of c-Myc by the proteasome inhibitor MG132 and also disturbed cycloheximide-treated c-Myc stability in Huh7 cells. Overall, these findings support the novel evidence that the pivotal role of c-Myc and SIRT1 is critically involved in Isoimperatorin-induced apoptosis in HCCs as potent molecular targets in liver cancer therapy.
Molecular Mechanism of Resistance to Alternaria alternata Apple Pathotype in Apple by Alternative Splicing of Transcription Factor MdMYB6-like
Authors: Zeng Xianqi , Wu Chao , Zhang Lulu , Lan Liming , Fu Weihong , Wang Sanhong
Published: April 2024

As a fruit tree with great economic value, apple is widely cultivated in China. However, apple leaf spot disease causes significant damage to apple quality and economic value. In our study, we found that MdMYB6-like is a transcription factor without auto-activation activity and with three alternative spliced variants. Among them, MdMYB6-like-β responded positively to the pathogen infection. Overexpression of MdMYB6-like-β increased the lignin content of leaves and improved the pathogenic resistance of apple flesh callus. In addition, all three alternative spliced variants of MdMYB6-like could bind to the promoter of MdBGLU H. Therefore, we believe that MdMYB6-like plays an important role in the infection process of the pathogen and lays a solid foundation for breeding disease-resistant cultivars of apple in the future.
Response of Industrial Hemp (Cannabis sativa L.) to Herbicides and Weed Control
Authors: Gitsopoulos Thomas , Tsaliki Eleni , Korres Nicholas E. , Georgoulas Ioannis , Panoras Ioannis , Botsoglou Despoina , Vazanelli Eirini , Fifis Konstantinos , Zisis Konstantinos
Published: April 2024

Industrial hemp is a continuously expanding crop; however, there has been limited research on its herbicide selectivity and weed control. Pendimethalin, s-metolachlor and aclonifen at 1137.5, 960 and 1800 g a.i. ha−1, respectively, were applied in field experiments in 2022 and 2023 in Greece to study the response of industrial hemp to pre-emergence (PRE) herbicides and record their efficacy on weeds. In 2023, each PRE herbicide was followed by the postemergence application of cycloxydim at 200 g a.i. ha−1 due to infestation of Sorghum halepense. In 2022, retardation in hemp growth was recorded by all PRE herbicide treatments, with there being a slight reduction in stand counts by pendimethalin and s-metolachlor and leaf yellowing by aclonifen in one the experiments. In 2023, no reductions in crop establishment and plant height were recorded, whereas leaf discoloration caused by aclonifen was less evident; cycloxydim did not affect hemp and perfectly controlled S. halepense. Despite the herbicide injury, hemp recovered and succeeded in higher biomass in both experiments at Thessaloniki and in higher seed production in the 2023 Thessaloniki experiment. This study showed that pendimethalin, s-metolachlor and aclonifen can be regarded as potential pre-emergence options with precautions in wet and light soils.
Study Protocol of a Prospective, Monocentric, Single-Arm Study Investigating the Correlation of Endograft Properties with Aortic Stiffness in Abdominal Aortic Aneurysm Patients Subjected to Endovascular Aortic Repair
Authors: Abatzis-Papadopoulos Manolis , Tigkiropoulos Konstantinos , Nikas Spyridon , Sidiropoulou Katerina , Alexou Christina , Stavridis Kyriakos , Karamanos Dimitrios , Kotsis Vasilios , Lazaridis Ioannis , Saratzis Nikolaos
Published: April 2024

The number of endovascular aortic repairs (EVARs) has surpassed the number of open surgical repairs of abdominal aortic aneurysms (AAAs) worldwide. The available commercial endoprostheses are composed of materials that are stiffer than the native aortic wall. As a consequence, the implantation of stent–graft endoprostheses during EVAR increases aortic rigidity and thus aortic stiffness, resulting in a decrease in abdominal aorta compliance. EVAR has been found to have a possibly harmful effect not only on heart functions but also on other vascular beds, including kidney function, due to the decrease in aortic compliance that it causes. Aortic stiffness is measured by various hemodynamic indices like the pulse wave velocity (PWV), the central aortic pressure (CAP), and the augmentation index (AIx). In the literature, there are increasing numbers of studies investigating the properties of endografts, which are strongly related to increases in aortic stiffness. However, there is a lack of data on whether there is a correlation between the length of various endografts implanted during EVAR and the increase in the PWV, CAP, and AIx postoperatively compared to the preoperative values. The aim of this prospective, observational, monocentric, single-arm study is to investigate the correlation between endograft length and the postoperative increase in the PWV, CAP, and AIx in patients subjected to EVAR. Additionally, this study intends to identify other endograft properties related to increases in the PWV, CAP, and AIx. Other endpoints to be studied are the existence of immediate postoperative myocardial and kidney injury after EVAR. The prediction of cardiovascular events caused by endograft-related increased aortic stiffness could contribute to the improvement of various endograft properties so that the impact of endografts on the native aortic wall can be minimized.
Transjugular Intrahepatic Portosystemic Shunt as a Bridge to Abdominal Surgery in Cirrhosis
Authors: Melandro Fabio , Parisse Simona , Ginanni Corradini Stefano , Cardinale Vincenzo , Ferri Flaminia , Merli Manuela , Alvaro Domenico , Pugliese Francesco , Rossi Massimo , Mennini Gianluca , Lai Quirino
Published: April 2024

Abdominal surgery is associated with high postoperative mortality and morbidity in cirrhotic patients. Despite improvements in surgical techniques, clinical management, and intensive care, the outcome could be influenced by the degree of portal hypertension, the severity of hepatopathy, or the type of surgery. Preoperative transjugular intrahepatic portosystemic shunt (TIPS) placement, in addition to medical therapy, plays an important role in managing the complications of portal hypertension such as ascites, hepatic encephalopathy, variceal bleeding or portal vein thrombosis. To date, the improvement of post-surgery outcomes in cirrhotic patients after TIPS placement remains unclear. Only observational data existing in the literature and prospective studies are urgently needed to evaluate the efficacy and safety of TIPS in this setting. This review aims to outline the role of TIPS as a tool in postoperative complications reduction in cirrhotic patients, both in the setting of emergency and elective surgery.
A Comparison between Core Stability Exercises and Muscle Thickness Using Two Different Activation Maneuvers
Authors: Tsartsapakis Ioannis , Bagioka Ioanna , Fountoukidou Flora , Kellis Eleftherios
Published: April 2024

Core stability training is crucial for competitive athletes, individuals who want to improve their health and physical performance, and those undergoing clinical rehabilitation. This study compared the ultrasound (US) muscle thickness of the abdominals and lumbar multifidus (LM) muscles between seven popular trunk stability exercises performed using hollowing and bracing maneuvers. Forty-four healthy young adults, aged between 21 and 32 years, performed a plank, bird dog, beast crawl, dead bug, Pilates tap, bridge, and side planks using the bracing and the hollowing maneuver. The thickness of the transversus abdominis (TrA), internal oblique (IO), and LM muscles was measured simultaneously using two ultrasound machines. Analysis of variance designs indicated that during hollowing, the bird dog and side plank exercises resulted in the greatest increase in the muscle’s relative thickness overall. The relative thickness of all muscles was significantly greater (p < 0.001) during hollowing (22.7 ± 7.80 to 106 ± 24.5% of rest) compared to bracing (18.7 ± 7.40 to 87.1 ± 20.9% of rest). The TrA showed the greatest increase in thickness (p < 0.001) compared to the IO and LM. Additionally, the IO had a greater increase in thickness (p < 0.001) than the LM. In conclusion, our findings indicate that the bird dog and side plank exercises, when performed with hollowing, showed the most significant total muscle thickness increase. Notably, the hollowing maneuver enhances the thickness of the TrA, IO, and LM muscles more than the bracing maneuver. This contributes to the discussion on optimal strategies for dynamic core stabilization.
Basal Cell Carcinoma: Diagnosis, Management and Prevention
Authors: Baba Peerzada Umar Farooq , Hassan Ashfaq ul , Khurshid Junaid , Wani Adil Hafeez
Published: April 2024

Basal cell carcinoma (BCC) is a slow-growing, locally aggressive, rarely metastasizing, low-grade cutaneous neoplasm that arises from the epidermal basal layer and invades the adjoining tissues. It is the most common skin cancer. It is fairly common in fair Caucasians and quite uncommon in dark-skinned populations. It contributes to 65–75% of cutaneous malignancies in whites and 20–30% in Asian Indians. The most important causal factors appear to be radiation exposure and genetic predisposition. It may present as a nonhealing lesion that occasionally bleeds or as a pruritic lesion with no symptoms. Tumours rarely spread to regional lymph nodes. The clinical appearances and morphology of BCC are diverse. Clinical types include nodular, cystic, superficial, pigmented, morphoeaform, (sclerosing), keratotic and fibroepithelioma of Pinkus. Most of the lesions appear on the head and neck, usually above the line joining the tragus and the angle of the mouth. A biopsy should be performed on all lesions suspected of BCC. The primary aim of treatment is the complete excision of the tumour tissue. Other treatment modalities include cryotherapy, immunomodulatory drugs, laser treatment or locally applicable chemotherapeutic agents. Prevention consists of lifestyle changes such as avoiding sunburn, tanning beds and prolonged direct sun exposure, shade seeking, sunscreen application on the skin, and physical barrier methods such as protective clothing, hats and sunglasses. Regular sunscreen use in childhood and adolescence seems more beneficial than in adulthood.
Coupling Changes in Runoff and Sediment and Their Relationships with Erosion Energy and Underlying Surface in the Wuding River Basin, China
Authors: Yang Qiannan , Gao Haidong , Han Yong , Li Zhanbin , Lu Kexin
Published: April 2024

Investigating the changes in the runoff and sediment coupling relationship in the Middle Yellow River Basin of China can not only deepen the understanding of soil loss control in arid areas, but also help solve key issues of regional ecological protection. Since the 1960s, soil- and water-conservation projects have been implemented in the Middle Yellow River Basin, inducing a significant reduction in runoff and sediment and changes in the relationship between runoff and sediment. The study identified the change points of coupling relationship between runoff and sediment in the Wuding River Basin (WRB) by constructing a diagnostic method based on coupling coordination degree and the Pettitt test; the study validated this using the Copula function and analyzed the impacts of erosion energy and underlying surface factors. The results showed the following: (1) the method based on coupling coordination degree and the Pettit test could accurately reflect the coupling relationship of runoff and sediment and identify two change points (1971 and 1996); (2) runoff and sediment in the WRB decreased gradually over three periods (P1, 1960–1970; P2, 1971–1995; P3, 1996–2020), with an average annual runoff of 15.34 × 108, 10.72 × 108, and 8.32 × 108 m3 and average annual sediment load of 1.84 × 108, 0.82 × 108, and 0.32 × 108 t, respectively; (3) the maximum possible joint design value of runoff and sediment under different return periods in P1 were all the highest, followed by P2 and P3, and the larger the return period, the higher the maximum possible joint design value; (4) runoff erosion power could promote runoff and sediment in PE (1960–2020), P1, P2 and P3 at a significant level, check dams and terrace could decrease runoff and sediment significantly in PE, and the highest contribution to runoff reduction was check dam (95.4%), while the highest contribution to sediment reduction was REP (93.8%). The study can provide a new way to analyze the changes in the runoff and sediment relationship and provide scientific support for runoff and sediment regulation in the Middle Yellow River Basin.
Development of Activity Data for Greenhouse Gas Inventory in Settlements in South Korea
Authors: Choi Sol-E , Kim Moonil , Son Yowhan , Jeon Seong-Woo , Lee Kyeong-Hak , Kim Whijin , Lee Sun-Jeoung , Lee Woo-Kyun
Published: April 2024

In South Korea, Agriculture, Forestry, and Other Land Use (AFOLU) collates greenhouse gas (GHG) inventories. However, the settlement category lacks a clear definition of land use and activity data. This study proposed a method for examining the settlement spatial extent and constructing activity data to estimate GHG emissions and absorption as a pilot calculation, as well as to provide data for land use classification. Utilizing cadastral maps (CDMs), settlement spatial extents were determined, with settlements occupying approximately 11% of the total land area in 2019, or 9% excluding overlaps. Activity data for settlements were established through a sampling method and analysis of aerial orthoimages from 2000 and 2019. After removing overlaps with digital forest type maps and smart farm maps, settlement activity data covered approximately 18.47% based on CDMs, or 12.66% excluding overlaps. In 2019, CO2 emissions and absorptions were estimated at 622.16 ktCO2yr−1 based on CDMs and 242.16 ktCO2yr−1, excluding overlaps. To enhance GHG inventory calculation consistency and compliance with TACCC principles, clear spatial extents for settlements must be established. This entails constructing activity data and assessing GHG inventories accordingly. GHG inventory statistics should also inform future nationally determined contributions.
Housing Market and Household Consumption in Urban China: A Debt Perspective
Authors: Lu Wencong , Zhu Kailing
Published: April 2024

Urbanization precipitates substantial economic transformations, with housing markets playing a pivotal role. The expansion of cities escalates housing demand, subsequently increasing housing debt, which is a crucial factor influencing household consumption. This study utilizes data from six rounds of the China Family Panel Studies (CFPS) spanning 2010 to 2020 to investigate the impact of housing debt on household consumption in urban China. Given the dynamics changes in housing debt status, as well as the endogeneity problems caused by reverse causality and selection bias between households’ debt decisions and consumption behavior, this study employs both the difference-in differences with multiple periods and groups (DIDM) model and the two-way fixed effects model. The results show that the average consumption expenditure of households with housing debt is 14% higher than those without. A 1% increase in housing debt size correlates with a 0.011% rise in consumption. Moreover, this positive effect is more pronounced among low-income households and those with older household heads, as well as those owning multiple properties. Compared to non-durable consumption, the impact of housing debt is more substantial on durable consumption. Further mechanism analysis reveals that the effect of housing debt on household consumption is primarily driven by an increase in housing assets, spurred by the rising housing prices. This study underscores the importance of integrating housing debt management within the framework of sustainable urban development, which not only ensures equitable access to housing but also fosters the promotion of sustainable consumption.
The Role of Sleep for Age-Related Differences in Neurobehavioral Performance
Authors: Tzischinsky Orna , Barel Efrat
Published: April 2024

This study investigated developmental changes from childhood to adulthood in neurobehavioral performance and sleep measures. While many studies have examined age-related changes between childhood and adolescence and from mid-to-late adulthood, young adulthood has been overlooked. The main aim of this study was to investigate the effects of sleep loss on developmental changes in neurobehavioral performance and sleepiness in a natural setting. A total of 119 children, adolescents, and young adults (38 children aged 6–9; 38 adolescents aged 13–19; and 43 young adults aged 20–27) wore an actigraph for a continuous five-weekday night. Subjective sleepiness (Karolinska Sleepiness Scale) and neurobehavioral performance (using the psychomotor vigilance test and the digit symbol substitution test) were measured on five school days. The results showed that adolescents and young adults outperformed children on both the digit symbol substitution test and the psychomotor vigilance test measures. However, adolescents committed more errors of commission on the psychomotor vigilance test and reported higher levels of subjective sleepiness. The results are discussed in relation to brain maturation in various cognitive functions.
The Influence of Ink Chemistry on the Microstructure Evolution and GHz RF Response of Printed Ag Transmission Lines
Authors: Summers Jason M. , Sakri Shambhavi , Chakma Nishako , Luyen Hung , Bujanda Andres , Parker Thomas , Tsang Harvey , Shepherd Nigel D.
Published: April 2024

High-frequency transmission is limited to the skin depth in metals. Because poor conductivity cannot be compensated for by increasing the conductor thickness as with DC, optimal transport properties are prerequisites for radio frequency (RF) use. Structural and chemical analyses of transmission lines printed using a traditional ink consisting of Ag nanoflakes in a dispersing phase revealed that optimized thermal treatments yielded thorough burnout of the binder, significant grain growth, elimination of the pore volume, and electrical responses that were comparable to values obtained for thermally evaporated, fully dense Ag controls. Specifically, a low DC resistivity of 2.3 μΩ·cm (1.4× bulk Ag) and RF transmission coefficients of 0.87 and 0.75 at 5 GHz and 10 GHz, respectively, were measured in the nanoflake Ag prints. Conversely, in transmission lines printed from a metal-organic decomposition ink, residual chemical contamination impeded diffusion and densification, yielding greater porosity, small grains that are pinned, and a degraded RF response. Reasonably good porosity approximations were obtained from a model based on percolation theory. The results indicate that contaminants at interfaces and pore surfaces impede diffusion, pore elimination, and full densification, and further, alter carrier dynamics and degrade RF response.
Dynamics in Explicit Gradient Elasticity: Material Frame-Indifference, Boundary Conditions and Consistent Euler–Bernoulli Beam Theory
Authors: Tsakmakis Charalampos , Broese Carsten , Sideris Stergios Alexandros
Published: April 2024

The paper is concerned with the boundary conditions of explicit gradient elasticity of Mindlin’s type in dynamics. It has been argued in an earlier paper that acceleration terms should not be present in the boundary tractions because of objectivity arguments. This is discussed in the present paper in more detail, and it is supplemented by assuming the validity of the principle of material frame indifference. Furthermore, new examples are discussed in order to illustrate that significant differences exist in the responses predicted by boundary tractions with and without acceleration terms.