bluuebunny/crossref_metadata_2025 huggingface dataset:
American college of radiology ovarian-adnexal reporting and data system ultrasound (O-RADS): Diagnostic performance and inter-reviewer agreement for ovarian masses in children
Authors: Wang Huimin , Wang Limin , An Siwei , Ma Qiuping , Tu Yanping , Shang Ning , Pan Yunxiang
Published: March 2023

Objective

To evaluate the diagnostic performance and inter-observer agreement of the American College of Radiology Ovarian-Adnexal Reporting and Data System Ultrasound (O-RADS) in the diagnosis of ovarian masses in children.

Methods

From June 2012 to December 2021, 163 ovarian masses in 159 patients with pathologic results were retrospectively analyzed. Each mass was classified into an O-RADS category according to the criteria. The diagnostic performance of O-RADS for detecting malignant ovarian masses was assessed using histopathology as the reference standard. Kappa (k) statistic was used to assess inter-observer agreement between a less-experienced and a well-experienced radiologist.

Results

Out of 163 ovarian masses, 18 (11.0%) were malignant and 145 (89.0%) were benign. The malignancy rates of O-RADS 5, O-RADS 4, and O-RADS 3 masses were 72.7%, 34.6%, and 4.8%, respectively. The area under the receiver operating characteristic curve was 0.944 (95% CI, 0.908–0.981). The optimal cutoff value for predicting malignant ovarian masses was  > O-RADS 3 with a sensitivity, specificity, and accuracy of 94.4%, 86.2% and 86.2% respectively. The inter-observer agreement of the O-RADS category was good (k = 0.777).

Conclusions

O-RADS has a high diagnostic performance for children with ovarian masses. It provides an effective malignant risk classification for ovarian masses in children, which shows high consistency between radiologists with different levels of experience.
Eating order and childhood obesity among preschoolers in China: A cross-sectional study
Authors: Dai Jin , Yang Jingyun , Fan Hailing , Wu Yixin , Wu Huilan , Wang Yun , Tung Tao-Hsin , Wang Lizhen , Zhang Meixian
Published: March 2023

Background

Early childhood is a critical period for dietary education and development of good eating habits. However, few studies have investigated the effect of eating order in children and childhood obesity in real-world settings.

Objective

To examine whether the order in which meats/fish or vegetables are consumed affects the risk of obesity in preschoolers.

Methods

We conducted a population-based cross-sectional study using a self-administered online survey on the lifestyle and health behaviors of preschoolers in Taizhou, China. A total of 3,200 parents were invited to take part in the survey, and 2,049 of them completed the questionnaire. Children were classified as having a normal weight, overweight, or obesity using the definitions provided by the International Obesity Task Force, and z-scores for body mass index were calculated. We divided the children's eating order at the beginning of the meal into two groups: “vegetables before meats/fish” or “meats/fish before vegetables”. We analyzed the relationship between what was consumed first at a meal and the overweight status of each child.

Results

No difference in body mass index was observed between the children eating meats/fish-first and the children eating vegetables-first during a meal. Children with parents who were affected by obesity were more likely to eat vegetables first. Among children of mothers with obesity, body mass index was significantly higher in the meats/fish-first group than that in the vegetable-first group (2.891 vs. 0.845, P = 0.007). In children whose mothers were affected by obesity, those that ate meats/fish first had a 12.21 times higher risk of being overweight compared with those that ate vegetables first (95% CI:1.22–121.74, P = 0.033).

Conclusion

Our findings suggest eating vegetables or meats/fish at the start of a meal does not affect weight status in preschoolers.
Myricetin attenuates hypoxic-ischemic brain damage in neonatal rats via NRF2 signaling pathway
Authors: Chen Tingting , Hu Yingying , Lu Liying , Zhao Qianlei , Tao Xiaoyue , Ding Bingqing , Chen Shangqin , Zhu Jianghu , Guo Xiaoling , Lin Zhenlang
Published: March 2023

Introduction: Hypoxic-ischemic encephalopathy (HIE) is a crucial cause of neonatal death and neurological sequelae, but currently there is no effective therapy drug for HIE. Both oxidative stress and apoptosis play critical roles in the pathological development of HIE. Myricetin, a naturally extracted flavonol compound, exerts remarkable effects against oxidative stress, apoptosis, and inflammation. However, the role and underlying molecular mechanism of myricetin on HIE remain unclear.

Methods: In this study, we established the neonatal rats hypoxic-ischemic (HI) brain damage model in vivo and CoCl2 induced PC12 cell model in vitro to explore the neuroprotective effects of myricetin on HI injury, and illuminate the potential mechanism.

Results: Our results showed that myricetin intervention could significantly reduce brain infarction volume, glia activation, apoptosis, and oxidative stress marker levels through activating NRF2 (Nuclear factor-E2-related factor 2) and increase the expressions of NRF2 downstream proteins NQO-1 and HO-1. In addition, the NRF2 inhibitor ML385 could significantly reverse the effects of myricetin.

Conclusion: This study found that myricetin might alleviate oxidative stress and apoptosis through NRF2 signaling pathway to exert the protective role for HI injury, which suggested that myricetin might be a promising therapeutic agent for HIE.
Long way to go: Progress of orphan drug accessibility in China from 2017 to 2022
Authors: Liu Jia , Yu Yue , Zhong Mingkang , Ma Chunlai , Shao Rong
Published: March 2023

Introduction: Over 400 million patients worldwide suffer from rare diseases. Access to orphan drugs is, therefore, crucial for this population. China has been actively working on improving orphan drug accessibility in the past decades, especially since 2018 when the First National List of Rare Diseases was announced. This study aimed to evaluate the current status of orphan drug accessibility in China regarding availability, daily cost, and affordability.

Methods: Market availability of orphan drugs in China was based on their approval status in China up to May 2022. Information on drug availability in hospitals and the cost of each drug from 2017 to 2021 was obtained from the database of the Science and Technology Development Center of the Chinese Pharmaceutical Association. Affordability was assessed by comparing the disposable daily income per capita to the cost of the defined daily dose of each drug.

Results: Market availability rate was 44.3% by May 2022, and the average delay in drug approval in China compared to its orphan approval in the United States of America was 5.9 ± 6.07 years. Drug availability in hospitals showed an upward trend, with availability in tertiary hospitals significantly higher than in secondary hospitals (~20%, p <0.0001). The eastern area was significantly higher in availability from 2019 onwards. Fifty-eight percent of the orphan drugs were still considered to have very low availability (<30%). The national median cost of the defined daily dose across all available orphan drugs had increased to 254.97 RMB in 2021. Only 34.98% of the orphan drugs were considered affordable when compared with the national average disposable daily income in 2021, and drug affordability decreased during the past 5 years.

Discussion: Changes in orphan drug regulations in China have enabled progress regarding the drugs’ market availability, but the current status of drug availability at hospitals, drug cost, and affordability were not optimal. Legislation for encouraging domestic drug development and novel payment schemes for high-value drugs are essential to further improve the availability and cost burden of orphan drugs in China
A survey of GNSS interference monitoring technologies
Authors: Qiao Jia , Lu Zukun , Lin Baojun , Song Jie , Xiao Zhibin , Wang Zhi , Li Baiyu
Published: March 2023

With the increasing economic and strategic significance of the global navigation satellite systems (GNSS), interference events also occur frequently. Interference monitoring technologies aim to monitor the interference that may affect the regular operation of the GNSS. Interference monitoring technologies can be divided into three parts: interference detection and recognition, interference source direction finding, and interference source location and tracking. Interference detection aims to determine whether interference exists. This paper introduces the classification of interference and the corresponding detection methods. The purpose of interference recognition is to recognize and classify interference. It is often combined with pattern recognition and machine learning algorithms. Interference source direction finding aims to estimate the direction of the interference signal. There are three kinds of methods: amplitude, phase, and spatial spectrum estimation. Interference source location aims to estimate the position of the interference signal. It is usually based on the received signal strength (RSS), time difference of arrival (TDOA), frequency difference of arrival (FDOA), angle of arrival (AOA) or direction of arrival (DOA). Interference source tracking aims to track moving interference sources, and it is generally based on Kalman filter theory. This paper summarizes the interference monitoring technologies and their latest progress. Finally, prospects for interference monitoring technologies are offered.
Pilot optimization for OFDM in the RSSB system
Authors: Chen Long , Yang Ziteng , Yu Qian
Published: March 2023

To increase the receiving sensitivity of the reversely single-sideband (RSSB) system with orthogonal frequency division multiplexing (OFDM) signal transmission, we use the improved pilot interval scheme and pilot power scheme in this system. The improved pilot interval scheme uses more pilots in areas where channel conditions are relatively good to avoid signal–signal beat interference (SSBI) issues for sub-carriers. The improved pilot power scheme compensates for frequency-selective fading by increasing the pilot power in areas where channel conditions are relatively poor. According to the simulation, the generated 60 GHz optical millimeter wave with 2.5 G bandwidth OFDM signal is delivered over 100 km standard single-mode fiber (SSMF). The improved pilot interval scheme and pilot power scheme can increase the system’s receiving sensitivity by 2 dB, respectively. These schemes can enhance the system’s performance without increasing the complexity of the algorithm and the costs of the RSSB system.
Identification of hypertrophy-modulating Cullin-RING ubiquitin ligases in primary cardiomyocytes
Authors: Fischer Maximillian , Jakab Moritz , Hirt Marc N. , Werner Tessa R. , Engelhardt Stefan , Sarikas Antonio
Published: March 2023

Cullin-RING ubiquitin ligases (CRL) regulate numerous biological processes in the heart and have been implicated in regulating cardiac hypertrophy. This study aimed to identify novel hypertrophy-modulating CRLs in cardiomyocytes (CM). A functional genomic approach using siRNA-mediated depletion and automated microscopy was employed to screen for cell size-modulating CRLs in neonatal rat CM. Screening hits were confirmed by 3H-isoleucine incorporation. Of 43 targets screened, siRNA-mediated depletion of Fbxo6, Fbxo45, and Fbxl14 resulted in decreased cell size, whereas depletion of Fbxo9, Fbxo25, Fbxo30, Fbxo32, Fbxo33, Cullin1, Roc1, Ddb1, Fbxw4, and Fbxw5 led to a markedly increased cell size under basal conditions. In CM stimulated with phenylephrine (PE), depletion of Fbxo6, Fbxo25, Fbxo33, Fbxo45, and Fbxw4 further augmented PE-induced hypertrophy. As a proof-of-concept, the CRLFbox25 was analysed by transverse aortic constriction (TAC) resulting in a 4.5-fold increase in Fbxo25 protein concentrations compared to control animals. In cell culture, siRNA-mediated depletion of Fbxo25 resulted in a ∼ 37% increase in CM cell size and ∼41% increase in 3H-isoleucine incorporation. Depleting Fbxo25 resulted in upregulation of Anp and Bnp. In summary, we identified 13 novel CRLs as positive or negative regulators of CM hypertrophy. Of these, CRLFbox25 was further characterized, as a potential modulator of cardiac hypertrophy.
Rainfed assessment of foxtail millet (Setaria italica L. beauv) germplasms through genotyping and principal component analysis
Authors: Singh Divya , Lawrence Kapil , Marker Shailesh , Bhattacharjee Indranil , Lawrence Reena , Choudhary Ravish , Ercisli Sezai , Karunakaran Rohini
Published: March 2023

Introduction

Foxtail millet (Setaria italica L. beauv) is an important crop in underdeveloped countries; however, yield levels are low. The use of varied germplasm in a breeding approach is critical for increasing productivity. Foxtail millet can be cultivated effectively in a wide range of environmental circumstances but it is best suited to hot and dry climates.

Methods

In the current study, multivariant traits were used to define 50 genotypes in the first year and 10 genotypes in the second year. The phenotypic correlations among all traits in the entire germplasm were assessed, and the data acquired for all quantitative characters were subjected to analysis of variance for augmented block design. Furthermore, WINDOWS STAT statistical software was used to carry out a principal component analysis (PCA). The presence of substantial variations in most symptoms was shown by analysis of variance.

Results

Genotypic coefficient of variation (GCV) projections for grain yields were the highest, followed by panicle lengths and biological yields. Plant height and leaf length had the highest PCV estimates, followed by leaf width. Low GCV and phenotypic coefficient of variation (PCV) were measured as leaf length and 50% flowering in days. According to the PCV study, direct selection based on characters, panicle weight, test weight, and straw weight had a high and positive effect on grain yield per plant in both the rainy and summer seasons, indicating the true relationship between these characters and grain yield per plant, which aids indirect selection for these traits and thus improves grain yield per plant. Variability in foxtail millet germplasm enables plant breeders to effectively select appropriate donor lines for foxtail millet genetic improvement.

Discussion

Based on the average performance of genotypes considered superior in terms of grain yield components under Prayagraj agroclimatic conditions, the best five genotypes were: Kangni-7 (GS62), Kangni-1 (G5-14), Kangni-6 (GS-55), Kangni-5 (GS-389), and Kangni-4 (GS-368).
Hyperspectral remote sensing for tobacco quality estimation, yield prediction, and stress detection: A review of applications and methods
Authors: Zhang Mingzheng , Chen Tian’en , Gu Xiaohe , Chen Dong , Wang Cong , Wu Wenbiao , Zhu Qingzhen , Zhao Chunjiang
Published: March 2023

Tobacco is an important economic crop and the main raw material of cigarette products. Nowadays, with the increasing consumer demand for high-quality cigarettes, the requirements for their main raw materials are also varying. In general, tobacco quality is primarily determined by the exterior quality, inherent quality, chemical compositions, and physical properties. All these aspects are formed during the growing season and are vulnerable to many environmental factors, such as climate, geography, irrigation, fertilization, diseases and pests, etc. Therefore, there is a great demand for tobacco growth monitoring and near real-time quality evaluation. Herein, hyperspectral remote sensing (HRS) is increasingly being considered as a cost-effective alternative to traditional destructive field sampling methods and laboratory trials to determine various agronomic parameters of tobacco with the assistance of diverse hyperspectral vegetation indices and machine learning algorithms. In light of this, we conduct a comprehensive review of the HRS applications in tobacco production management. In this review, we briefly sketch the principles of HRS and commonly used data acquisition system platforms. We detail the specific applications and methodologies for tobacco quality estimation, yield prediction, and stress detection. Finally, we discuss the major challenges and future opportunities for potential application prospects. We hope that this review could provide interested researchers, practitioners, or readers with a basic understanding of current HRS applications in tobacco production management, and give some guidelines for practical works.
Fertilizer source and application method influence sugarcane production and nutritional status
Authors: Castro Sérgio Gustavo Quassi de , Coelho Anderson Prates , Castro Saulo Augusto Quassi de , Souza Chiachia Thais Regina de , Castro Rosilaine Araldi de , Lemos Leandro Borges
Published: March 2023

Introduction

The contrasting weather conditions throughout the sugarcane harvest period in south-central Brazil (April to November) influence fertilization management in sugarcane ratoon.

Methods

Through field studies carried out over two cropping seasons, we aimed to compare the performance of sugarcane at sites harvested in the early and late periods of the harvest season as a function of fertilizer sources associated with application methods. The design used in each site was a randomized block in a 2 x 3 factorial scheme; the first factor consisted of fertilizer sources (solid and liquid), and the second factor consisted of application methods (above the straw, under the straw, and incorporated into the middle of the sugarcane row).

Results

The fertilizer source and application method interacted at the site harvested in the early period of the sugarcane harvest season. Overall, the highest sugarcane stalk and sugar yields at this site were obtained with the incorporated application applying liquid fertilizer and under straw applying solid fertilizer, with increments of up to 33%. For the site harvested in the late period of the sugarcane harvest season, the liquid fertilizer promoted a 25% higher sugarcane stalk yield compared to the solid fertilizer in the crop season with low rainfall in the spring, while in the crop season with normal rainfall, there were no differences between treatments.

Discussion

This demonstrates the importance of defining fertilization management in sugarcane as a function of harvest time, thereby promoting greater sustainability in the production system
Molecular characterization of cross-kingdom RNA interference in Botrytis cinerea by tomato small RNAs
Authors: Qin Si , Veloso Javier , Puccetti Guido , van Kan Jan A. L.
Published: March 2023

Previous studies have suggested that plants can modulate gene expression in pathogenic fungi by producing small RNAs (sRNAs) that can be translocated into the fungus and mediate gene silencing, which may interfere with the infection mechanism of the intruder. We sequenced sRNAs and mRNAs in early phases of the Solanum lycopersicum (tomato)-Botrytis cinerea interaction and examined the potential of plant sRNAs to silence their predicted mRNA targets in the fungus. Almost a million unique plant sRNAs were identified that could potentially target 97% of all fungal genes. We selected three fungal genes for detailed RT-qPCR analysis of the correlation between the abundance of specific plant sRNAs and their target mRNAs in the fungus. The fungal Bcspl1 gene, which had been reported to be important for the fungal virulence, showed transient down-regulation around 20 hours post inoculation and contained a unique target site for a single plant sRNA that was present at high levels. In order to study the functionality of this plant sRNA in reducing the Bcspl1 transcript level, we generated a fungal mutant that contained a 5-nucleotide substitution that would abolish the interaction between the transcript and the sRNA without changing the encoded protein sequence. The level of the mutant Bcspl1 transcript showed a transient decrease similar to wild type transcript, indicating that the tomato sRNA was not responsible for the downregulation of the Bcspl1 transcript. The virulence of the Bcspl1 target site mutant was identical to the wild type fungus.
Unique gene duplications and conserved microsynteny potentially associated with resistance to wood decay in the Lauraceae
Authors: Tian Xue-Chan , Guo Jing-Fang , Yan Xue-Mei , Shi Tian-Le , Nie Shuai , Zhao Shi-Wei , Bao Yu-Tao , Li Zhi-Chao , Kong Lei , Su Guang-Ju , Mao Jian-Feng , Lin Jinxing
Published: March 2023

Wood decay resistance (WDR) is marking the value of wood utilization. Many trees of the Lauraceae have exceptional WDR, as evidenced by their use in ancient royal palace buildings in China. However, the genetics of WDR remain elusive. Here, through comparative genomics, we revealed the unique characteristics related to the high WDR in Lauraceae trees. We present a 1.27-Gb chromosome-level assembly for Lindera megaphylla (Lauraceae). Comparative genomics integrating major groups of angiosperm revealed Lauraceae species have extensively shared gene microsynteny associated with the biosynthesis of specialized metabolites such as isoquinoline alkaloids, flavonoid, lignins and terpenoid, which play significant roles in WDR. In Lauraceae genomes, tandem and proximal duplications (TD/PD) significantly expanded the coding space of key enzymes of biosynthesis pathways related to WDR, which may enhance the decay resistance of wood by increasing the accumulation of these compounds. Among Lauraceae species, genes of WDR-related biosynthesis pathways showed remarkable expansion by TD/PD and conveyed unique and conserved motifs in their promoter and protein sequences, suggesting conserved gene collinearity, gene expansion and gene regulation supporting the high WDR. Our study thus reveals genomic profiles related to biochemical transitions among major plant groups and the genomic basis of WDR in the Lauraceae.
Meta-QTL and haplo-pheno analysis reveal superior haplotype combinations associated with low grain chalkiness under high temperature in rice
Authors: Kumari Anita , Sharma Divya , Sharma Priya ,   Sahil, Wang Chaoxin , Verma Vibha , Patil Arun , Imran Md , Singh Madan Pal , Kumar Kuldeep , Paritosh Kumar , Caragea Doina , Kapoor Sanjay , Chandel Girish , Grover Anil , Jagadish S. V. Krishna , Katiyar-Agarwal Surekha , Agarwal Manu
Published: March 2023

Chalk, an undesirable grain quality trait in rice, is primarily formed due to high temperatures during the grain-filling process. Owing to the disordered starch granule structure, air spaces and low amylose content, chalky grains are easily breakable during milling thereby lowering head rice recovery and its market price. Availability of multiple QTLs associated with grain chalkiness and associated attributes, provided us an opportunity to perform a meta-analysis and identify candidate genes and their alleles contributing to enhanced grain quality. From the 403 previously reported QTLs, 64 Meta-QTLs encompassing 5262 non-redundant genes were identified. MQTL analysis reduced the genetic and physical intervals and nearly 73% meta-QTLs were narrower than 5cM and 2Mb, revealing the hotspot genomic regions. By investigating expression profiles of 5262 genes in previously published datasets, 49 candidate genes were shortlisted on the basis of their differential regulation in at least two of the datasets. We identified non-synonymous allelic variations and haplotypes in 39 candidate genes across the 3K rice genome panel. Further, we phenotyped a subset panel of 60 rice accessions by exposing them to high temperature stress under natural field conditions over two Rabi cropping seasons. Haplo-pheno analysis uncovered haplotype combinations of two starch synthesis genes, GBSSI and SSIIa, significantly contributing towards the formation of grain chalk in rice. We, therefore, report not only markers and pre-breeding material, but also propose superior haplotype combinations which can be introduced using either marker-assisted breeding or CRISPR-Cas based prime editing to generate elite rice varieties with low grain chalkiness and high HRY traits.
Competition between FLS and DFR regulates the distribution of flavonols and proanthocyanidins in Rubus chingii Hu
Authors: Lei Ting , Huang Jun , Ruan Haixiang , Qian Wei , Fang Zhou , Gu Chunyang , Zhang Niuniu , Liang Yaxuan , Wang Ziyun , Gao Liping , Wang Yunsheng
Published: March 2023

Rubus chingii Hu is a berry plant of the genus Rubus of the Rosaceae family, which has high nutritional and medicinal value and is rich in flavonoids. Flavonol synthase (FLS) and dihydroflavonol 4-reductase (DFR) compete for the common substrate dihydroflavonols to regulate the metabolic flux of flavonoids. However, the competition between FLS and DFR based on enzyme is rarely reported. Here, we isolated and identified two FLS genes (RcFLS1 and RcFLS2) and one DFR gene (RcDFR) from Rubus chingii Hu. RcFLSs and RcDFR were highly expressed in stems, leaves, and flowers, although the flavonol accumulation in these organs was significantly higher than that of proanthocyanidins (PAs). The recombinant RcFLSs demonstrated bifunctional activities via hydroxylation and desaturation at the C-3α position having a lower Michaelis constant (Km) for dihydroflavonols than RcDFR. We also found that a low concentration of flavonols could significantly inhibit RcDFR activity. To investigate the competitive relationship between RcFLSs and RcDFR, we used a prokaryotic expression system (E. coli) to co-express these proteins. The transgenic cells expressing recombinant proteins were incubated with substrates, and the reaction products were analyzed. Furthermore, two transient expression systems (tobacco leaves and strawberry fruits) and a stable genetic system (Arabidopsis thaliana) were used to co-express these proteins in vivo. The results showed that RcFLS1 was dominant in the competition with RcDFR. Our results demonstrated that the competition between FLS and DFR regulated the metabolic flux distribution of flavonols and PAs, which will be of great significance for the molecular breeding of Rubus plants.
Crop domestication disrupts intercropping benefits: A case study from barley–faba bean mixture under contrasting P inputs
Authors: Tang Xiaoyan , Wu Zhengwu , Hu Siliu , Chen Guangdeng , Huang Rong , Wu Yingjie , Li Bing , Tao Qi , Jin Kemo , Wang Changquan , Wen Zhihui
Published: March 2023

How crop domestication mediates root functional traits and trait plasticity in response to neighboring plants is unclear, but it is important for selecting potential species to be grown together to facilitate P uptake. We grew two barley accessions representing a two-stage domestication process as a sole crop or mixed with faba bean under low and high P inputs. We analyzed six root functional traits associated with P acquisition and plant P uptake in five cropping treatments in two pot experiments. The spatial and temporal patterns of root acid phosphatase activity were characterized in situ with zymography at 7, 14, 21, and 28 days after sowing in a rhizobox. Under low P supply, wild barley had higher total root length (TRL), specific root length (SRL), and root branching intensity (RootBr) as well as higher activity of acid phosphatase (APase) in the rhizosphere, but lower root exudation of carboxylates and mycorrhizal colonization (MC), relative to domesticated barley. In response to neighboring faba bean, wild barley exhibited larger plasticity in all root morphological traits (TRL, SRL, and RootBr), while domesticated barley showed greater plasticity in root exudates of carboxylates and colonization by mycorrhiza. Wild barley with greater root morphology-related trait plasticity was a better match with faba bean than domesticated barley, indicated by higher P uptake benefits in wild barley/faba bean than domesticated barley/faba bean mixtures under low P supply. Our findings indicated that the domestication of barley disrupts the intercropping benefits with faba bean through the shifts of root morphological traits and their plasticity in barley. Such findings provide valuable information for barley genotype breeding and the selection of species combinations to enhance P uptake.
Does brand community participation lead to early new product adoption? The roles of networking behavior and prior purchase experience
Authors: Jiang Ying , Liao Junyun , Pang Jiecong , Hu Hsin-Li
Published: March 2023

Introduction

Consumers’ adoption behavior is critical to the success of new products, but the effects of brand communities on new product adoption have rarely been investigated. In this study, we draw on network theory to examine how consumer participation in brand communities (in terms of participation intensity and social networking behaviors) affects the adoption of new products.

Methods

We collected longitudinal data from 8,296 members of an online community of a well-known smartphone brand to assess the factors influencing new product adoption.

Results

The results from applying a hazard model indicated that brand community participation increases the speed of adoption of new products. The positive effect of members’ out-degree centrality on new product adoption was found to be significant, but in-degree centrality only had an effect when users had previous purchasing experience.

Discussion

These findings extend the literature by revealing how new products are disseminated across brand communities. The study also makes theoretical and practical contributions to the literature on brand community management and product marketing.
Global trends in research related to emergence delirium, 2012–2021: A bibliometric analysis
Authors: Wang Kenru , Cai Jiehui , Du Ruiming , Wu Jiaxuan
Published: March 2023

Introduction

Emergence delirium is a kind of mental disorder during the early awakening period after general anesthesia, which is manifested as the combination of perceptual disturbance and psychomotor agitation. It is an independent risk factor for postoperative delirium and even long-term postoperative cognitive decline, which often affects the postoperative outcome and deserves the attention of clinical anesthesiologists. There are many studies on emergence delirium, but the quantity and quality of these studies are unclear. Therefore, we conducted a bibliometric analysis of studies on emergence delirium between January 2012 and December 2021. Through the analysis of relevant literature, the research hotspots and trends of emergence delirium are understood, which can provide a reference for future research.

Methods

We searched the Web of Science Core Collection (WoSCC) for original articles and reviews related to emergence delirium published between 2012 and 2021, and collected a variety of bibliographic elements, including annual publications, authors, countries/regions, institutions, journals, and keywords. Three different science-based tools (CiteSpace, VOSviewer and Bibliometrix) were used for this comprehensive analysis.

Results

From January 2012 to December 2021, a total of 912 emergence delirium (ED) related literature were published, including 766 original research articles and 146 review articles. The number of publications has increased every year except 2016. The United States published 203 articles, ranking first with China, followed by South Korea (95 articles). The United States is also the country with the most citations (4,508), and Yonsei Univ is the most productive institution. The most published journal was PEDIATRIC ANESTHESIA, with the highest h and g index. LEE JH is the most influential author in this field.

Discussion

“Children, emergence agitation, delirium, dexmedetomidine” are the hot topics in this field in recent years. The bibliometric analysis in this field will provide the future direction for the study of emergence delirium for clinicians.
The role of sexting in couple wellbeing for Italian women during the second wave of the COVID-19 pandemic
Authors: Bonfanti Rubinia Celeste , Garro Maria , Lavanco Gioacchino , Ruggieri Stefano
Published: March 2023

The social isolation due to the COVID-19 pandemic had an impact on the sexuality and quality of life of people around the world. A particularly negative effect was detected on women’s sexual health. As a consequence, many women began to use social media not only to stay in touch with their social networks, but as a way of maintaining sexual contact. The main aim of this research is to observe the positive effects of sexting in women’s wellbeing as a strategy to manage the negative effects of a condition of forced isolation. We collected all our data between November 2020 and March 2021 during a period of strict restrictions in Italy due to the second wave of the COVID-19 pandemic. In Study 1, the relationship between loneliness, sexting behaviors, and sexual satisfaction was tested on 312 adult women. The results showed the mediator role of motivation for sexting in the relationship between loneliness and sexual satisfaction. In Study 2, 342 adult women were organized into two groups (women who had sexting at least once during the second wave of the pandemic = 203, and women who did not have sexting during the pandemic = 139) and were assessed on a couple’s wellbeing (intimacy, passion, commitment, and couple satisfaction) and electronic surveillance. The results show that women who had sexting during isolation had higher scores on intimacy, passion, couple satisfaction, and electronic surveillance. These findings suggest the important role of sexting as an adaptive coping strategy during particular conditions of social isolation.
Can biomarkers be used to diagnose attention deficit hyperactivity disorder?
Authors: Chen Hui , Yang Yang , Odisho Diana , Wu Siqi , Yi Chenju , Oliver Brian G.
Published: March 2023

Currently, the diagnosis of attention deficit hyperactivity disorder (ADHD) is solely based on behavioral tests prescribed by the Diagnostic and Statistical Manual of Mental Disorders, 5th Edition (DSM-5). However, biomarkers can be more objective and accurate for diagnosis and evaluating treatment efficacy. Thus, this review aimed to identify potential biomarkers for ADHD. Search terms “ADHD,” and “biomarker” combined with one of “protein,” “blood/serum,” “gene,” and “neuro” were used to identify human and animal studies in PubMed, Ovid Medline, and Web of Science. Only papers in English were included. Potential biomarkers were categorized into radiographic, molecular, physiologic, or histologic markers. The radiographic analysis can identify specific activity changes in several brain regions in individuals with ADHD. Several molecular biomarkers in peripheral blood cells and some physiologic biomarkers were found in a small number of participants. There were no published histologic biomarkers for ADHD. Overall, most associations between ADHD and potential biomarkers were properly controlled. In conclusion, a series of biomarkers in the literature are promising as objective parameters to more accurately diagnose ADHD, especially in those with comorbidities that prevent the use of DSM-5. However, more research is needed to confirm the reliability of the biomarkers in larger cohort studies.
Relationship of serum estradiol and progesterone with symptoms and sex difference in schizophrenia: A cross-sectional study in Iran
Authors: Shahini Najmeh , Salimi Zanireh , Kiani Dorsa , Raftari Ahmad , Ziaee Maliheh
Published: March 2023

Background

Schizophrenia is a devastating disease characterized by frequent relapses, cognitive decline, and emotional and functional disability, with unknown causes. The phenomenology and clinical course of schizophrenic disorders are different between the two genders, which is thought to be related mainly to the effects of steroid sex hormones on the nervous system. Regarding inconsistencies in the studies, we aimed to compare the levels of estradiol and progesterone between schizophrenia patients and healthy individuals.

Methods

This cross-sectional study was conducted on 66 patients referred to the specialized clinical psychiatric ward of a teaching hospital in the north of Iran, for 5 months in 2021. Thirty-three schizophrenia patients confirmed by a psychiatrist based on DSM5 criteria were included in the case group, and 33 individuals without a psychiatric disease were included in the control group. We completed a demographic information checklist for each patient, along with the Simpson-Angus extrapyramidal side effect scale (SAS) for drug side effects and the positive and negative syndrome scale (PANSS) for the severity of the disease symptoms. Then, a 3-ml blood sample was taken from each participant to determine the serum levels of estradiol and progesterone. The data were analyzed by SPSS16 software.

Results

Thirty-four (51.5%) and 32 (48.5%) participants in this study were male and female, respectively. The mean serum level of estradiol was 22.33 ± 13.65 pm/dl in schizophrenia patients and 29.36 ± 21.32 pm/dl in the control group, showing no significant difference between the two groups (P = 0.4). However, the mean serum level of progesterone was significantly lower in schizophrenia patients (0.37 ± 1.39 pm/dl) than in control subjects (3.15 ± 5.73 pm/dl) (P < 0.001). The PANSS and SAS scores were not significantly correlated with the level of sex hormones (P > 0.05). Serum estradiol and progesterone levels based on sex significantly differed between the two groups (except for female estradiol).

Conclusion

Considering the hormonal differences between schizophrenia patients and control subjects, determining hormonal levels in these patients and using complementary hormonal therapies with estradiol or similar compounds can be beneficial as the starting point of schizophrenia treatment, where therapeutic responses can draw the future developmental framework.
Shame, self-disgust, and envy: An experimental study on negative emotional response in borderline personality disorder during the confrontation with the own face
Authors: Biermann Miriam , Schulze Anna , Vonderlin Ruben , Bohus Martin , Lyssenko Lisa , Lis Stefanie
Published: March 2023

Background

A markedly negative self-image and pervasive shame proneness have consistently been associated with borderline personality disorder (BPD). The present experimental study investigated the intensity of negative emotional responses with a focus on shame in BPD compared to healthy control persons (HCs) during an experimental paradigm promoting self-awareness, self-reflection, and self-evaluation. Furthermore, the relationship between levels of state shame during the experiment and shame proneness in BPD compared to HCs was examined.

Methods

A sample of 62 individuals with BPD and 47 HCs participated in the study. During the experimental paradigm, participants were presented with photos of (i) the own face, (ii) the face of a well-known person, and (iii) of an unknown person. They were asked to describe positive facets of these faces. Participants rated the intensity of negative emotions induced by the experimental task as well the pleasantness of the presented faces. Shame-proneness was assessed using the Test of the Self-Conscious Affect (TOSCA-3).

Results

Individuals with BPD experienced significantly higher levels of negative emotions than HCs both before and during the experimental task. While HC participants responded to their own face particularly with an increase in shame compared to the other-referential condition, the BPD patients responded above all with a strong increase of disgust. Furthermore, the confrontation with an unknown or well-known face resulted in a strong increase of envy in BPD compared to HC. Individuals with BPD reported higher levels of shame-proneness than HCs. Higher levels of shame-proneness were related to higher levels of state shame during the experiment across all participants.

Conclusion

Our study is the first experimental study on negative emotional responses and its relationship to shame proneness in BPD compared to HC using the own face as a cue promoting self-awareness, self-reflection, and self-evaluation. Our data confirm a prominent role of shame when describing positive features of the own face, but they emphasize also disgust and envy as distinct emotional experience characterizing individuals with BPD when being confronted with the self.
Characterizing the physical and mental health profile of children, adolescents and adults with autism spectrum disorder in Spain
Authors: Vidriales-Fernández Ruth , Plaza-Sanz Marta , Hernández-Layna Cristina , Verde-Cagiao María , Benito-Ruiz Guillermo , Carvajal-Molina Fernando
Published: March 2023

Introduction

Autistic men and women are more likely to experience health issues than the general population, although the available epidemiological studies addressing co-occurrence conditions are limited. This is the first Spanish epidemiologic study addressing the health profile and poor-health exacerbating factors in individuals of all ages with autism spectrum disorder (ASD).

Methods

We analyzed 2,629 registries extracted from Autism Spain’s sociodemographic registry (November 2017–May 2020). A descriptive health data analysis was conducted to assess the prevalence of other conditions associated to ASD in the Spanish population. Nervous system disorders (12.9%), mental health diagnoses (17.8%), and other comorbidities (25.4%) were reported. Men-to-women ratio was 4:1.

Results

Women, elder individuals and those with intellectual disability (ID) were at an increased risk of health comorbidities and psychopharmacological exposure. Women were also more prone to severe intellectual and functional impairment. Nearly all individuals had difficulties in their adaptative functioning, especially those with ID (50% of the population). Almost half of the sample received psychopharmacological treatments starting from infancy and early childhood, mostly antipsychotics and anticonvulsants.

Discussion

This study represents an important first approach to the health status of autistic people in Spain and can contribute to the development of public policies and innovative health strategies.
Parsing neurobiological heterogeneity of the clinical high-risk state for psychosis: A pseudo-continuous arterial spin labelling study
Authors: Oliver Dominic , Davies Cathy , Zelaya Fernando , Selvaggi Pierluigi , De Micheli Andrea , Catalan Ana , Baldwin Helen , Arribas Maite , Modinos Gemma , Crossley Nicolas A. , Allen Paul , Egerton Alice , Jauhar Sameer , Howes Oliver D. , McGuire Philip , Fusar-Poli Paolo
Published: March 2023

Introduction

The impact of the clinical high-risk for psychosis (CHR-P) construct is dependent on accurately predicting outcomes. Individuals with brief limited intermittent psychotic symptoms (BLIPS) have higher risk of developing a first episode of psychosis (FEP) compared to individuals with attenuated psychotic symptoms (APS). Supplementing subgroup stratification with information from candidate biomarkers based on neurobiological parameters, such as resting-state, regional cerebral blood flow (rCBF), may help refine risk estimates. Based on previous evidence, we hypothesized that individuals with BLIPS would exhibit increased rCBF compared to APS in key regions linked to dopaminergic pathways.

Methods

Data from four studies were combined using ComBat (to account for between-study differences) to analyse rCBF in 150 age- and sex-matched subjects (n = 30 healthy controls [HCs],n = 80 APS,n = 20 BLIPS andn = 20 FEP). Global gray matter (GM) rCBF was examined in addition to region-of-interest (ROI) analyses in bilateral/left/right frontal cortex, hippocampus and striatum. Group differences were assessed using general linear models: (i) alone; (ii) with global GM rCBF as a covariate; (iii) with global GM rCBF and smoking status as covariates. Significance was set atp < 0.05.

Results

Whole-brain voxel-wise analyses and Bayesian ROI analyses were also conducted. No significant group differences were found in global [F(3,143) = 1,41,p = 0.24], bilateral frontal cortex [F(3,143) = 1.01,p = 0.39], hippocampus [F(3,143) = 0.63,p = 0.60] or striatum [F(3,143) = 0.52,p = 0.57] rCBF. Similar null findings were observed in lateralized ROIs (p > 0.05). All results were robust to addition of covariates (p > 0.05). No significant clusters were identified in whole-brain voxel-wise analyses (p > 0.05FWE). Weak-to-moderate evidence was found for an absence of rCBF differences between APS and BLIPS in Bayesian ROI analyses.

Conclusion

On this evidence, APS and BLIPS are unlikely to be neurobiologically distinct. Due to this and the weak-to-moderate evidence for the null hypothesis, future research should investigate larger samples of APS and BLIPS through collaboration across large-scale international consortia.
Striatal increase of dopamine receptor 2 density in idiopathic and syndromic mouse models of autism spectrum disorder
Authors: Chhabra Stuti , Nardi Leonardo , Leukel Petra , Sommer Clemens J. , Schmeisser Michael J.
Published: March 2023

Autism spectrum disorder (ASD) comprises a wide range of neurodevelopmental phenotypes united by impaired social interaction and repetitive behavior. Environmental and genetic factors are associated with the pathogenesis of ASD, while other cases are classified as idiopathic. The dopaminergic system has a profound impact in the modulation of motor and reward-motivated behaviors, and defects in dopaminergic circuits are implicated in ASD. In our study, we compare three well-established mouse models of ASD, one idiopathic, the BTBR strain, and two syndromic, Fmr1 and Shank3 mutants. In these models, and in humans with ASD, alterations in dopaminergic metabolism and neurotransmission were highlighted. Still, accurate knowledge about the distribution of dopamine receptor densities in the basal ganglia is lacking. Using receptor autoradiography, we describe the neuroanatomical distribution of D1 and D2 receptors in dorsal and ventral striatum at late infancy and adulthood in the above-mentioned models. We show that D1 receptor binding density is different among the models irrespective of the region. A significant convergence in increased D2 receptor binding density in the ventral striatum at adulthood becomes apparent in BTBR and Shank3 lines, and a similar trend was observed in the Fmr1 line. Altogether, our results confirm the involvement of the dopaminergic system, showing defined alterations in dopamine receptor binding density in three well-established ASD lines, which may provide a plausible explanation to some of the prevalent traits of ASD. Moreover, our study provides a neuroanatomical framework to explain the utilization of D2-acting drugs such as Risperidone and Aripiprazole in ASD.