bluuebunny/crossref_metadata_2025 huggingface dataset:
Entre transmodalité et multimodalité : quelle(s) lecture(s) de Madame Bovary ?
Authors: Cuin Marie-Hélène
Published: March 2023

L’article présente un dispositif d’enseignement de lecture littéraire mis en œuvre pour une classe de Seconde autour de l’étude en œuvre intégrale de Madame Bovary de Flaubert. Il interroge la nature et les usages des objets sémiotiques secondaires transmodaux et multimodaux en réception et en production, mis en dialogue avec les écrits textuels. La séquence intègre des objets transmodaux en réception afin de faciliter l’entrée dans l’œuvre princeps , sa contextualisation et son actualisation ; elle inclut également un écrit médiatique multimodal considéré comme un texte du lecteur transmodal. Si l’expérimentation menée au sein de deux classes au profil différent a permis de mettre en évidence une implication accrue des élèves pour l’œuvre patrimoniale et sa lecture, en revanche la multiplication des supports et des modalités de lecture semble accentuer les difficultés des élèves les plus fragiles et aggraver les inégalités socioculturelles.
Lectures et contre-lectures du Journal d’un chat assassin en CE2-CM1 : un éclairage interprétatif par la BD ?
Authors: Castagnet-Caignec Sonia
Published: March 2023

La contribution s’attache à analyser comment une œuvre et son adaptation transmodale peuvent contribuer à développer des compétences de compréhension-interprétation chez des élèves de fin de cycle 2, début de cycle 3 du cursus primaire. L’étude s’appuie sur une œuvre recommandée par le ministère de l’Éducation nationale, Journal d’un chat assassin , court roman illustré d’Anne Fine, adapté en BD par Véronique Deiss. Trois classes ont étudié les deux ouvrages dans une séquence d’enseignement-apprentissage en lecture littéraire, suivant deux protocoles didactiques différents : succession des ouvrages et alternance des ouvrages avec dominante roman ou BD. Les retours sur la séance finale ainsi que sur les obstacles rencontrés par les élèves, notamment lors de la lecture-compréhension d’images transposant un récit à la première personne, permettent de dégager les bénéfices et les limites d’une lecture comparée d’une œuvre et de son adaptation pour un jeune public scolaire.
La réception de l’œuvre de Jean Remy dans les pays lusophones
Authors: Balsa Casimiro
Published: March 2023

Cette contribution balise les orientations de la réception de l’œuvre de Jean Remy en langue portugaise en tenant compte du fait qu’il est un auteur pluriel , avant d’être un auteur collectif . Son œuvre est vaste et éclectique, avec de multiples points d’ancrage empiriques qui débouchent sur différents cadres interprétatifs. Il est tout aussi important de tenir compte du cadre académique dans lequel J. Remy a travaillé. Peu de ses travaux sont traduits en portugais et il est souvent cité en français. Pour l’essentiel, l’appropriation de son œuvre en langue portugaise se fait à l’intérieur d’un « triangle » constitué par : sa démarche méthodologique, l’intentionnalité théorique qui marque l’ensemble de son œuvre et l’approche pédagogique qui en découle.
La « cuisine commune » de Fives : pratiques sociales et transactions au cœur d’un espace participatif lillois
Authors: Gibout Christophe , Fauquette Alexandre
Published: March 2023

Interrogeant pendant près de quatre années un dispositif participatif fondé sur l’alimentation dans un quartier défavorisé de Lille, cet article met au jour la moralisation douce qui est mise en œuvre par les autorités locales et les animateurs du dispositif. Face à la montée de protestations et afin de garantir la continuité du projet, des transactions spatiales et sociales et des compromis pratiques ont été initiés. Ils traduisent tout à la fois la capacité d’action des plus faibles et la capacité d’imposition des plus puissants.
Santerre contre Santerre. Écritures d’un procès conjugal (1879-1880)
Authors: Glaumaud-Carbonnier Marion
Published: March 2023

En 1879, la séparation des époux Santerre surexcite la curiosité parisienne, friande de l’intimité dévoilée d’un couple qui se déchire. Lequel des deux récits de la vie conjugale qui s’affrontent à la barre faut-il croire ? À travers l’examen des factums judiciaires, des plaidoiries des avocats et de la littérature dérivée de cette affaire, cet article, s’intéressant à la machine fictionnelle d’un procès, entend explorer la passion jurislittéraire qui enfièvre la société de cette époque. À l’heure où l’on débat de la morale républicaine et de l’immoralité naturaliste, ce procès pose en effet aux contemporains une question fondamentale : le droit est-il une littérature comme une autre ? Contribuant à l’analyse des liens entre droit et littérature, cette étude de cas, au plus près des narrations procédurales, propose ainsi de réfléchir à ce que lire un procès en littéraire veut dire.
Représentations de la justice révolutionnaire dans le roman historique
Authors: Durand Isabelle
Published: March 2023

À travers un corpus français et anglais ( La Comtesse de Charny et Le Chevalier de Maison-Rouge de Dumas, Un conte de deux villes de Dickens, et la saga du Mouron Rouge d’Emma Orczy), l’article s’interroge sur les liens entre représentation du Tribunal révolutionnaire et conception de la Révolution (notamment pensée contre-révolutionnaire anglaise). Souvent point d’aboutissement des intrigues romanesques et objet de fascination, la scène de procès devant le Tribunal révolutionnaire concentre un certain nombre de clichés : les foules et leur violence, l’animalité et le sadisme du peuple et des jurés, la dimension carnavalesque des procès. De telles descriptions révèlent de la caricature, ce qui amène à une interrogation sur la légitimité de cette justice révolutionnaire. Les auteurs y répondent différemment, selon leur culture politique et le rapport qu’ils entretiennent avec l’Histoire.
Amniotic Membrane Patching to Assist Closure of Recalcitrant Macular Holes
Authors: Lee Jessica , Chua Michael , Jansen Michael , Giovinazzo Jerome , Powell Brittany , Deobhakta Avnish , Wong Amanda , Zhu Daniel , Ferrone Philip J. , Gentile Ronald , Landa Gennady , Dayan Alan , Pollack Aryeh , Rosen Richard
Published: March 2023

Background and Objective:

This study reports a case series of patients with persistent macular holes (MHs) who underwent human amniotic membrane subretinal placement to achieve successful anatomic MH closure.

Patients and Methods:

This was a retrospective case series of patients with persistently open full-thickness MHs who underwent human amniotic membrane placement. Patients were observed up to 6 months postoperatively.

Results:

Ten patients were included. The mean preoperative best-corrected visual acuity was 1.6 logMAR (20/800). Postoperatively, mean best-corrected visual acuity improved to 1.3 logMAR (20/400) at 1 month and 1.1 logMAR (20/250) by the 3- and 6-month visits. In all cases, the MH appeared closed at the 1-week visit and remained closed at their last follow-up. Optical coherence tomography showed closure in all cases. No adverse events were reported.

Conclusions:

Human amniotic membrane sub-retinal placement may serve as a useful surgical technique to assist in the closure of recalcitrant macular holes.

[
Ophthalmic Surg Lasers Imaging Retina
2023;54:218–222.]
Conservation laws analysis of nonlinear partial differential equations and their linear soliton solutions and Hamiltonian structures
Authors: Ju Long , Zhou Jian , Zhang Yufeng
Published: March 2023

This article mainly uses two methods of solving the conservation laws of two partial differential equations and a system of equations. The first method is to construct the conservation law directly and the second method is to apply the Ibragimov method to solve the conservation laws of the target equation systems, which are constructed based on the symmetric rows of the target equation system. In this paper, we select two equations and an equation system, and we try to apply these two methods to the combined KdV-MKdV equation, the Klein-Gordon equation and the generalized coupled KdV equation, and simply verify them. The combined KdV-MKdV equation describes the wave propagation of bound particles, sound waves and thermal pulses. The Klein-Gordon equation describes the nonlinear sine-KG equation that simulates the motion of the Josephson junction, the rigid pendulum connected to the stretched wire, and the dislocations in the crystal. And the coupled KdV equation has also attracted a lot of research due to its importance in theoretical physics and many scientific applications. In the last part of the article, we try to briefly analyze the Hamiltonian structures and adjoint symmetries of the target equations, and calculate their linear soliton solutions.
On constrained minimizers for Kirchhoff type equations with Berestycki-Lions type mass subcritical conditions
Authors: Hu Jing , Sun$ ^{} $ Jijiang
Published: March 2023

In this paper, for given mass $ m > 0 $, we focus on the existence and nonexistence of constrained minimizers of the energy functional

   \begin{document}$ \begin{equation*} I(u): = \frac{a}{2}\int_{\mathbb{R}^3}\left|\nabla u\right|^2dx+\frac{b}{4}\left(\int_{\mathbb{R}^3}\left|\nabla u\right|^2dx\right)^2-\int_{\mathbb{R}^3}F(u)dx \end{equation*} $\end{document}

on $ S_m: = \left\{u\in H^1(\mathbb{R}^3):\, \|u\|^2_2 = m\right\}, $where $ a, b > 0 $ and $ F $ satisfies the almost optimal mass subcritical growth assumptions. We also establish the relationship between the normalized ground state solutions and the ground state to the action functional $ I(u)-\frac{\lambda}{2}\|u\|_2^2 $. Our results extend, nontrivially, the ones in Shibata (Manuscripta Math. 143 (2014) 221–237) and Jeanjean and Lu (Calc. Var. 61 (2022) 214) to the Kirchhoff type equations, and generalize and sharply improve the ones in Ye (Math. Methods. Appl. Sci. 38 (2015) 2603–2679) and Chen et al. (Appl. Math. Optim. 84 (2021) 773–806).
A novel B-spline collocation method for Hyperbolic Telegraph equation
Authors: Kırlı Emre
Published: March 2023

The present study is concerned with the construction of a new high-order technique to establish approximate solutions of the Telegraph equation (TE). In this technique, a novel optimal B-spline collocation method based on quintic B-spline (QBS) basis functions is constructed to discretize the spatial domain and fourth-order implicit method is derived for time integration. Test problems are considered to verify the theoretical results and to demonstrate the applicability of the suggested technique. The error norm $ L_{\infty } $ and the rate of spatial and temporal convergence are computed and compared with those of techniques available in the literature. The obtained results show the improvement and efficiency of the proposed scheme over the existing ones. Also, it is obviously observed that the experimental rate of convergence is almost compatible with the theoretical rate of convergence.
A generalized Halpern-type forward-backward splitting algorithm for solving variational inclusion problems
Authors: Dechboon Premyuda , Adamu Abubakar , Kumam Poom
Published: March 2023

In this paper, we investigate the problem of finding a zero of sum of two accretive operators in the setting of uniformly convex and $ q $-uniformly smooth real Banach spaces ($ q > 1 $). We incorporate the inertial and relaxation parameters in a Halpern-type forward-backward splitting algorithm to accelerate the convergence of its sequence to a zero of sum of two accretive operators. Furthermore, we prove strong convergence of the sequence generated by our proposed iterative algorithm. Finally, we provide a numerical example in the setting of the classical Banach space $ l_4(\mathbb{R}) $ to study the effect of the relaxation and inertial parameters in our proposed algorithm.
Brain image fusion-based tumour detection using grey level co-occurrence matrix Tamura feature extraction with backpropagation network classification
Authors: Bhavani R. , Vasanth K.
Published: March 2023

Most challenging task in medical image analysis is the detection of brain tumours, which can be accomplished by methodologies such as MRI, CT and PET. MRI and CT images are chosen and fused after preprocessing and SWT-based decomposition stage to increase efficiency. The fused image is obtained through ISWT. Further, its features are extracted through the GLCM-Tamura method and fed to the BPN classifier. Will employ supervised learning with a non-knowledge-based classifier for picture classification. The classifier utilized Trained databases of the tumour as benign or malignant from which the tumour region is segmented via k-means clustering. After the software needs to be implemented, the health status of the patients is notified through GSM. Our method integrates image fusion, feature extraction, and classification to distinguish and further segment the tumour-affected area and to acknowledge the affected person. The experimental analysis has been carried out regarding accuracy, precision, recall, F-1 score, RMSE and MAP.
Ukrajinsko-slovenski in slovensko-ukrajinski slovar / Українсько-словенський та словенсько-український словник
Authors: Dumka Vladimir
Published: March 2023

The Ukrainian-Slovene dictionary and its Slovene-Ukrainian version (compiled by Vladimir Dumka) contains 24,352 Ukrainian and 22,515 Slovene entries. The Ukrainian language part was revised by Ukrainian colleague Dr Liudmyla Danylenko from the Institute of Linguistics O.O. Potebnje, National Academy of Sciences of Ukraine. The lexicographical refinement of the Slovene language was done by Dr Silvo Torkar from the Institute for the Slovene Language Fran Ramovš ZRC SAZU. To ensure the highest level of translations, the glossary was reviewed by the permanent court interpreter, Natalija Glažar Berčič. The Ukrainian part of the dictionary relies predominantly on the eleven-volume explanatory dictionary of the Ukrainian language Slovnik ukrainian movi (1970-1981), while the Slovene part draws on the dictionary of the Slovene literary language (Slovar slovenskega knjižnega jezika, 1970-1991) and the Slovene orthography (Slovenski pravopis, 2001). The dictionary is intended for Slovenes learning Ukrainian or traveling to Ukraine, as well as for Ukrainians visiting or moving to Slovenia or those who want to learn the Slovene language. This is a much-needed contribution to improving the contacts between the two nations and their cultures.
Sporočilnost poštnih znamk v obdobju družbenopolitične tranzicije na območju nekdanje Jugoslavije – 2. del
Authors: Gosar Anton
Published: March 2023

Članek analizira in obravnava motive, valuto in pisavo na priložnostnih poštnih znamkah Zvezne republike Jugoslavije, Severne Makedonije, Bosne in Hercegovine, Kosova, Državne skupnosti Srbije in Črne Gore, Srbije ter Črne gore. V petletnem raziskovalnem obdobju pred in po prelomu politične/družbene in teritorialne strukture so njihove poštne uprave natisnile in izdale 1035 priložnostnih poštnih znamk. Temeljna hipoteza tega dela raziskave izhaja iz predpostavke, da se – vzporedno z družbenopolitičnimi spremembami – sporočilnost poštnih znamk spreminja. Raziskava je hipotezo deloma potrdila, saj več kot petina priložnostnih znamk (237 znamk ali 23 %) posreduje politične ali domoljubne vsebine, ki po številu sledijo sporočilom, tesno povezanim z nacionalno kulturo in znanostjo (31 %). Čeprav se novonastale države z izrazji na znamkah (motiv, pisava, valuta) zamejujejo v lasten kulturni krog, pa je iz nekaterih izdaj poštnih znamk mogoče razbrati željo po (ponovni) vključitvi v večnacionalno skupnost – Evropsko unijo. Na temelju preverjanj izrazja na poštnih znamkah v obdobju nastajanja in/ali dezintegracije držav v jugovzhodni Evropi ugotavljamo, da sporočilnost na poštnih znamkah dodatno utrjuje etnični nacionalizem. //

Expressions of postage stamps in times of political and social transition on the territory of former Yugoslavia – part 2

The article analyses and discusses the visuals, currency and writing on the postage stamps of the Federal Republic of Yugoslavia, North Macedonia, Bosnia and Herzegovina, Kosovo, the State Union of Serbia and Montenegro, Serbia and Montenegro. During the five-year research period, before and after the break in the political/social and territorial structure, we’ve found out that postal administrations in all these countries issued 1,035 postage stamps related to an occasion. In the journal Geografski vestnik, a similar analysis was conducted on stamps printed in socialist Yugoslavia (SFRJ) and sovereign nation-states of Slovenia and Croatia, now part of the European Union. The basic hypothesis of the research stems from the assumption that – parallel to socio-political changes – the messages on postage stamps are changing. The research confirmed the hypothesis, as more than 1/5 of stamps convey political content (237 postage stamps – 23%); these are complemented by messages closely related to the national culture, art and science (31%). On own postage stamps nation-states of South-Eastern Europe limit themselves with visuals, alphabets and currency to their cultural circle; but on rare occasions the desire to (again) (re)integrate into a multinational community, like the European Union, is smouldering. Based on the verification of the expression on postage stamps during the period of formation and/or disintegration of states in South-Eastern Europe, we conclude that the messages on postage stamps strengthen ethnic nationalism.
Notranjska v regionalizaciji Slovenije
Authors: Nared Janez
Published: March 2023

V prispevku smo preučili, kakšna delitev na pokrajine bi bila z vidika Notranjske najbolj primerna. Analizirali smo centralnost naselij, dnevno mobilnost delovne sile in sektorske členitve, s spletnim anketiranjem pa smo preverili, kje anketiranci koristijo storitve regionalnega pomena ter kaj menijo o predlaganih regionalizacijah. Rezultati nakazujejo na dve skupini občin – občine vzhodno od nekdanje rapalske meje, ki gravitirajo proti Ljubljani, ter občine zahodno od nekdanje rapalske meje, ki gravitirajo proti Postojni in Kopru. Medtem ko anketiranci iz slednjih prednost dajejo povezovanju z obalno-kraškimi občinami, nasprotujejo pa povezovanju z osrednjeslovenskimi občinami, anketiranci iz občin vzhodno od nekdanje rapalske meje v ospredje postavljajo povezovanje notranjskih občin in oblikovanje samostojne Notranjske pokrajine. Izhajajoč iz strokovnih meril in deljenega mnenja anketirancev oblikovanje Notranjske kot samostojne pokrajine ni smiselno. //
Inner Carniola in the regionalization of Slovenia
This article investigates which regionalization would be the most suitable with regard to the region of Inner Carniola. It analyzes the area’s central settlements, workforce commuting, and sectoral divisions, as well as information from an online survey determining where respondents use services of regional importance and what their attitudes are about proposed regionalizations. The results indicate two groups of municipalities: municipalities east of the former Rapallo border that are oriented toward Ljubljana, and municipalities west of the former Rapallo border that are oriented toward Postojna and Koper. Respondents from the latter give priority to connections with the coastal municipalities, but they are opposed to strengthening connections with municipalities in central Slovenia, whereas respondents from the municipalities east of the former Rapallo border prioritize connections with other municipalities in Inner Carniola and the creation of Inner Carniola as a separate region. Based on professional criteria and the divided opinion of the respondents, the designation of Inner Carniola as a separate region does not make sense.
Fatores dinâmicos locais e ocorrências de tempestades com granizo
Authors: Balicki Marcos , Andrade Aparecido Ribeiro de , Hornes Karin Linete
Published: March 2023

As precipitações de granizo são comuns em regiões de clima tropical e/ou subtropical, pois normalmente ocorrem em ambientes com alta instabilidade atmosférica e em maiores amplitudes térmicas. Nesse sentido, a busca pelo entendimento das causas e dos efeitos desse tipo de precipitação para a sociedade é fundamental, já que pode ajudar na definição de estratégias para mitigar os impactos negativos. Diante disso, este estudo objetivou relacionar a quantidade e o tamanho de granizos com cinco fatores influenciadores na ocorrência desse tipo de precipitação: horário, temperatura, estação do ano, tipos de tempestade (isoladas ou organizadas) e a quantidade de chuva (mm). A pesquisa foi realizada durante o período de janeiro de 2018 a dezembro de 2020 no município de Laranjeiras do Sul, estado do Paraná, Brasil. Ao todo, foram coletados dados de sete eventos. A metodologia se amparou na utilização de ferramentas tais como o pluviômetro analógico, o termômetro, paquímetros, e imagens de radar obtidas junto ao Sistema Meteorológico do Paraná (SIMEPAR). Os dados foram organizados em tabelas para cada evento, sendo efetuada uma análise estatística básica. Os resultados indicaram que as maiores quantidades de granizo precipitaram quando a temperatura do ar estava em torno de 18ºC, com pluviosidade média superior a 1 mm/min e sob intensos vendavais. Quanto aos tamanhos, notou-se que granizos maiores de 20 mm de diâmetro ocorreram preferencialmente em dias mais quentes no inverno, com temperatura em torno de 20,8ºC.
A valorização no ensino de Geografia das práticas socioespaciais dos jovens na cidade
Authors: Moura Bruna de , Gomes Marquiana de Freitas Vilas Boas
Published: March 2023

A categoria juventude abarca distintos modos do que é ser jovem, pois não é possível caracterizar a juventude com uma definição universal, já que há uma heterogeneidade presente na vivência juvenil, perpassando contextos e circunstâncias distintas. Diante disso, a juventude deve ser entendida em sua pluralidade. A maneira como o professor se utiliza dos saberes cotidianos adquiridos pelos jovens para promover o ensino de Geografia pode interferir na compreensão deles a respeito da cidade, e no pensamento geográfico que se constrói entre a experiência e os conteúdos escolares. Promover atividades que unam o conhecimento cotidiano dos jovens escolares aos conteúdos escolares traz a possibilidade de produzir significados aos conceitos e conteúdos, e pode potencializar diferentes raciocínios. Nesse contexto, a presente pesquisa busca contribuir com as discussões acerca das práticas socioespaciais de jovens escolares na cidade, verificando como esses jovens, a partir de suas vivências, podem desenvolver raciocínios geográficos integrando conhecimento cotidiano ao conteúdo escolar. O objetivo central do trabalho é descrever as características dos jovens escolares pesquisados e propor sugestões de atividades pedagógicas que o professor pode utilizar para o ensino de Geografia a partir do protagonismo juvenil e da formação de conceitos, visando à formação do pensamento geográfico e desenvolvimento da cidadania. A metodologia da pesquisa integrou análise de questionários e elaboração de propostas pedagógicas para o ensino. Na pesquisa, foram realizadas sugestões de atividades pedagógicas que o professor pode utilizar para o ensino de Geografia a partir do protagonismo juvenil e da formação de conceitos. Esse protagonismo e a formação de conceitos mostram que o papel principal do professor, nessa situação, é criar condições para que os jovens entendam a cidade em que vivem e se reconheçam enquanto sujeitos, ao mesmo tempo em que possam lutar por suas demandas.
Proposta didático-pedagógica para o ensino de Geomorfologia na Educação Básica
Authors: Santos Anderson Felipe Leite dos
Published: March 2023

O homem é um agente transformador do modelado terrestre e sua ação sobre o relevo ocasiona problemas de diferentes naturezas, envolvendo o social, o ambiental, o econômico e o político. Neste trabalho, a antropogeomorfologia urbana ganha destaque, visto que se evidencia como um ramo da Geomorfologia essencial para o entendimento da ação antrópica no modelado das cidades, permitindo a compreensão dos processos geomorfológicos, a previsão de fenômenos catastróficos e o planejamento urbano-ambiental das cidades. Este trabalho tem como objetivo apresentar uma proposta didático-pedagógica para discutir a antropogeomorfologia urbana na Geografia escolar, uma vez que é importante que os alunos compreendam mais sobre a ação antrópica no relevo em diferentes escalas geográficas. A proposta apresentada possui abordagem qualitativa, do tipo relato de experiência, e foi construída para o trabalho de conclusão da disciplina “Impacto Humano no Meio Físico: Prognose e Dimensionamento”, oferecida no semestre 2021. 1, em formato remoto, pelo Programa de Pós-Graduação em Geografia Física da Universidade de São Paulo (PPGF-USP). Os resultados alcançados mostram que é importante a utilização de propostas didático-pedagógicas diferenciadas que aproximem o conteúdo Geomorfologia da realidade vivenciada pelos alunos, com a finalidade de que possam perceber as mudanças e perigos que a ação antrópica pode causar na paisagem. Além disso, o resultado leva a uma reflexão e uma crítica acerca do currículo em Licenciatura em Geografia, que nem sempre contempla propostas metodológicas que sejam mais pertinentes ao cotidiano dos alunos e isso, por sua vez, não prepara adequadamente os licenciandos para o exercício pleno da docência.
Mapeamento das áreas suscetíveis às inundações e aos alagamentos no município de Jaboatão dos Guararapes, Pernambuco
Authors: Pessoa Neto Amaury Gouveia , Silva Simone Rosa da , Barbosa Ioná Maria Beltrão Rameh
Published: March 2023

O fenômeno da urbanização no Brasil se desenvolveu de maneira acelerada e desordenada, alterando significativamente as condições de uso do solo e resultando em consideráveis mudanças no ciclo hidrológico. O reflexo desse processo se tornou um dos principais problemas enfrentados pela cidade, que é o aumento da ocorrência de inundações e alagamentos. Assim, o presente trabalho buscou mapear as áreas suscetíveis a esses tipos de desastres hidrológicos no município de Jaboatão dos Guararapes, em Pernambuco, utilizando o AHP (Processo Analítico Hierárquico), em um Sistema de Informações Geográficas (SIG). Para isso, foram selecionados sete fatores que contribuem para os desastres hidrológicos que foram processados no software QGIS (v. 3.10.9), reclassificados e dispostos à álgebra de mapas. Dessa forma, foi possível confeccionar o mapa das áreas suscetíveis às inundações e aos alagamentos para a região estudada, sendo observado que as regiões que apresentaram maiores propensões aos desastres hidrológicos são as mais próximas aos corpos hídricos e que possuem baixas declividades e altitudes, fortes intervenções antrópicas e solos com baixa capacidade de infiltração. A utilização do AHP quanto ao mapeamento das áreas suscetíveis às inundações e alagamentos no município de Jaboatão dos Guararapes se demonstrou uma alternativa vantajosa quanto à aplicabilidade e viabilidade, permitindo tomadas de decisões por gestores e/ou profissionais da área.
Validation of Ligand Tetramers for the Detection of Antigen-Specific Lymphocytes
Authors: Fitzpatrick Kristin S , Degefu Hanna N , Poljakov Katrina , Bibby Madeleine G , Remington Allison J , Searles Tyler G , Gray Matthew D , Boonyaratanakornkit Jim , Rosato Pamela C , Taylor Justin J
Published: March 2023

The study of Ag-specific lymphocytes has been a key advancement in immunology over the past few decades. The development of multimerized probes containing Ags, peptide:MHC complexes, or other ligands was one innovation allowing the direct study of Ag-specific lymphocytes by flow cytometry. Although these types of study are now common and performed by thousands of laboratories, quality control and assessment of probe quality are often minimal. In fact, many of these types of probe are made in-house, and protocols vary between laboratories. Although peptide:MHC multimers can often be obtained from commercial sources or core facilities, few such services exist for Ag multimers. To ensure high quality and consistency with ligand probes, we have developed an easy and robust multiplexed approach using commercially available beads able to bind Abs specific for the ligand of interest. Using this assay, we have sensitively assessed the performance of peptide:MHC and Ag tetramers and have found considerable batch-to-batch variability in performance and stability over time more easily than using murine or human cell-based assays. This bead-based assay can also reveal common production errors such as miscalculation of Ag concentration. This work could set the stage for the development of standardized assays for all commonly used ligand probes to limit laboratory-to-laboratory technical variation and experimental failure caused by probe underperformance.
Effects of osmotic stress on Listeria monocytogenes ATCC 7644: persistent cells and heat resistance
Authors: Nalbone Luca , Sorrentino Giorgia , Giarratana Filippo , Schiopu- Mariean Aurelian , Ziino Graziella , Giuffrida Alessandro
Published: March 2023

Persistent bacteria are a microbial subpopulation that, exposed to bactericidal treatment, is killed at a slower rate than the rest of the population they are part of. They can be triggered either following stress or stochastically without external signals. The hallmark of persistent bacteria is the biphasic killing curve, a sign that, within a microbial population, two subpopulations are inactivated at a different rate. Furthermore, when plated into a fresh medium and in the absence of stressors, persistent bacteria typically remain in the lag phase longer before resuming active replication. This study aims to evaluate in vitro whether the formation of persistent cells in a strain of Listeria monocytogenes can be triggered by exposure to osmotic stress and if this phenomenon can increase heat resistance in the bacterial population. In a first experiment, the lag time distribution of a L. monocytogenes strain grown in a 6% NaCl broth was evaluated using the software ScanLag. A stationary phase broth culture was inoculated on agar plates placed on an office scanner inside an incubator at 37°C. The plates were scanned every 20’ for 4 days and the acquired images were automatically elaborated with the aid of MatLab software in order to evaluate the appearance times of every single colony. The experiment was also carried out on a control culture obtained by growing the strain in the broth without salt. In a second experiment, the same broth cultures, after proper dilutions to rebalance NaCl concentration, were subjected to a heat treatment at 51°C and the death curves obtained were parameterized using the GinaFit system. Results showed that the lag phase of 31.40% of the salt culture colonies was long enough to suppose the formation of persistent bacteria. Analyses of the thermal survival curves showed that the shoulder and tail model was the one that best represented the inactivation trend of the salt culture, unlike the control culture, whose trend was essentially linear. Results of the present study show how exposure to salt could induce the formation of persistent bacteria in a L. monocytogenes strain. The last raises concerns as persistent cells may not only be undetected with common analytical techniques but they even show a greater heat resistance.
Prognostic value of neutrophil/lymphocyte ratio, lymphocyte/monocyte ratio, lactate dehydrogenase, and mean platelet volume in the diagnosis of patients with diffuse large B-cell lymphoma
Authors: Bakirtas Mehmet , Basci Semih , Candır Burcu Aslan , Ulu BaharUncu , Yaman Samet , Yiğenoğlu Tuğçe Nur , Dal Mehmet Sinan , Çakar MerihKızıl , Altuntaş Fevzi
Published: March 2023

Background

Diffuse large B-cell lymphoma (DLBCL), a heterogeneous type of lymphoma, encompasses various biologic abnormalities and numerous morphologic variants, showing several clinical findings and responses to treatments. Lactate dehydrogenase (LDH) is a well-established diagnostic and prognostic marker for DLBCL, and neutrophil/lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR), and mean platelet volume (MPV) have been shown to have prognostic values in several malignancies.

Objectives

In the study, we examined the prognostic value of LMR, NLR, LDH, and MPV in the stage and prognosis of DLBCL by analyzing the data of patients treated with rituximab-based chemotherapies.

Patients and methods

A total of 188 patients diagnosed as having DLBCL between January 2012 and January 2020 were selected. DLBCL stages were categorized as early and late, international prognostic index was categorized as below and above 4, and the treatment response was categorized as responders and nonresponders. NLR, LMR, LDH, MPV, and other factors predicting these outcomes were analyzed.

Results

Logistic regression analysis showed that the factors influencing stage of DLBCL were NLR [P=0.009, odds ratio (OR)=1.220, 95% confidence interval (CI): 1.050–1.417] and LDH (P=0.001, OR=0.286, 95% CI: 0.146–0.561). The factor influencing international prognostic index score was LMR (P=0.001, OR=6.226, 95% CI: 2.092–18.533). Factors influencing response were R-CHOP treatment (P=0.001, OR=0.181, 95% CI: 0.068–0.478) and stage (P=0.005, OR=18.306, 95% CI: 2.383–140.607).

Conclusion

The pretreatment LMR, NLR, LDH, and MPV values may affect the stage and prognosis of DLBCL, which showed influences on the treatment response.
Predictive value of neutrophil-to-lymphocyte, lymphocyte-to-monocyte, and platelet-to-lymphocyte ratios in adult and pediatric acute lymphoblastic leukemia patients
Authors: Youssef Mariam K. , Radwan Rania A. , Makkeyah Sara M. , Taha Sara I.
Published: March 2023

Background

Methods used for prognostication of acute lymphoblastic leukemia (ALL) are expensive; discovering low-cost prognostic factors is challenging.

Objectives

This study aimed to explore the prognostic role of baseline neutrophil-to-lymphocyte (NLR), lymphocyte-to-monocyte (LMR), and platelet-to-lymphocyte (PLR) ratios in predicting the response to end of induction chemotherapy in ALL patients.

Patients and methods

We included 44 adult patients and 47 pediatric patients who were newly diagnosed with ALL. All participants were subjected to a full history taking and a thorough medical examination. Laboratory investigations included complete blood count (CBC) with differential count analysis, with calculation of NLR, LMR, and PLR; bone marrow examination; conventional cytogenetic analysis; and immunophenotyping. Patients were followed until the end of the induction phase, and their response to treatment was assessed.

Results

Among the adult patients, 63.6% showed complete remission at the end of induction; their baseline CBC showed significantly lower NLR (P=0.001) and higher LMR (P=0.013). On the other hand, 66% of the pediatric patients showed good response to induction chemotherapy; their baseline CBC showed significantly lower NLR (P<0.001), greater LMR (P=0.0134), and lower PLR (P=0.017). NLR more than or equal to 1, LMR less than or equal to 2.846, and PLR more than or equal to 39.1 were able to discriminate adult patients who will respond to induction chemotherapy, similarly NLR more than or equal to 1, LMR less than or equal to 3.286, and PLR more than or equal to 10 among pediatric patients.

Conclusion

Our research discovered that the rise in NLR and PLR, together with the decline of LMR at ALL diagnosis, could predict future resistance to the routinely used induction protocols, and the need for intensification regimens.
Cortactin
Authors: El-Razzaz Mostafa K. , Ahmed Tamer M. , Eissa Deena S. , Abdalla NourElhoda H. , Shaheen Mohammed A. , Mohamed Haydi S.
Published: March 2023

Background

Chronic myeloid leukemia (CML) is a clonal myeloproliferative disease characterized by leukocytosis and an accumulation of granulocytes and their precursors. Cortactin is an actin-binding protein substrate of Src kinase. High cortactin expression in many hematological malignancies has been correlated with adverse prognostic factors.

Aim

The aim of our study was to measure cortactin levels in patients with CML at diagnosis and correlate such levels with other prognostic factors.

Patients and methods

This is a case–control study that was executed at hematology unit, Ain-Shams University Hospital during the period between January 2021 and October 2021. The study included 25 newly diagnosed patients with chronic phase CML and 25 healthy controls. Accelerated phase and blast crisis were excluded from the study.

Results

Cortactin level at diagnosis was higher in the patients group compared with the control group (71.04 ± 20.04 vs. 36.8 ± 11.6%, P<0.001). Cortactin level was significantly higher in patients who did not achieve complete hematological remission (CHR) at 3 months in comparison with those who achieved CHR (88.49 ± 8.02 vs. 61.23 ± 17.98, P<0.001). Patients who failed to attain CHR at 3 months had a significantly worse prognostic score at diagnosis using Sokal, Hasford, and ELTS scores (P=0.016, 0.035, and 0.009, respectively), but this did not apply to EUTOS score (P=0.089).

Conclusion

Higher cortactin levels are associated with delayed CHR in newly diagnosed patients with chronic phase CML, and it can be used as a prognostic marker for patients with CML at diagnosis.
Unwelcome guests in monocytes
Authors: Rath Asish , Vishwanathan Ganesh K.
Published: March 2023

Disseminated histoplasmosis is a serious and life-threatening opportunistic infection in patients with HIV/AIDS. An early diagnosis and appropriate therapy can save lives. The diagnosis predominantly relies on clinical suspicion, radiological investigations, and histopathology. Rarely, Histoplasma can be identified in peripheral blood monocytes or neutrophils. However, a careful peripheral blood smear examination is needed and can result in an early diagnosis. We present a case of a 28-year old young man suffering from HIV/AIDS, who could be diagnosed with disseminated histoplasmosis with a careful peripheral blood smear examination.
Low leukocyte count in Langya henipavirus and severity of infection
Authors: Mungmunpuntipantip Rujittika , Wiwanitkit Viroj
Published: March 2023

Aim

The aim is to determine the leukocyte count in Langya henipavirus infection.

Background

The ‘Langya henipavirus’ is a brand new viral pathogen that first appeared in 2022. In the Chinese cities where this new virus was discovered, a big population was found with this infection. Despite the fact that the particular mode of transmission is unknown, zoonosis looks to be a possibility. Clinical medicine has little knowledge of the clinical symptoms of a recent infection.

Methods

The authors look into the signs of Langya henipavirus infections, including a low leukocyte count.

Results

Although the actual source of the new disease's white blood cell dysfunction is unknown, a range of immunological or nonimmunological variables could be involved.

Conclusion

A low leukocyte count, according to current studies, can signal a severe infection. More research is required to validate this finding.
Augmented Berlin-Frankfurt-Munster versus GRAALL-2003 in young adults with Philadelphia-negative, B-cell acute lymphoblastic leukemia
Authors: Ghobrial Fady E.I. , Elkerdawy Heidi , Abdelhakiem Mostafa , El-Ashwah Shaimaa
Published: March 2023

Background

Acute lymphoblastic leukemia (ALL) is a heterogeneous group of diseases characterized by clonal proliferation of lymphoblasts. Improvement in the outcome of ALL in adolescents and young adult (AYA) patients remains one of the challenging problems in ALL treatment.

Aim

This study was conducted to compare the outcome of polychemotherapy regimens, augmented Berlin-Frankfurt-Munster (ABFM) regimen and GRAALL-2003, in Philadelphia-negative, B-cell ALL in AYA patients.

Patients and methods

A single-center retrospective study was performed on AYA patients with Philadelphia-negative, B-cell ALL who were diagnosed between 2013 and 2019.

Results

A total of 37 patients were included (27 males and 10 females) with a mean age of 22.3 years. Complete remission (CR) rate in the ABFM group was 94.1 versus 75% in the GRALL-2003 group (P=0.1). There was no statistically significant difference regarding relapse rate between both regimens (P=0.2). Regarding toxicities, there was no statistically significant difference between the two regimens apart from ICU admission rate, which was statistically significantly higher in the GRAALL-2003 group compared with the ABFM group (P=0.048); however, it had no influence on the overall survival.

Conclusion

The outcome of both polychemotherapy regimens, ABFM and GRAALL-2003, was comparable regarding CR rate, relapse rate, overall survival, and toxicity profile apart from a noticeable increased rate of ICU admission in GRAALL-2003 regimen, which makes ABFM regimen the more feasible option in treatment.
Telemedicine in patients with hematological diseases during the coronavirus disease 2019 (Covid-19) pandemic
Authors: Rawi Rasha , Salah Marwa , Mattar Mervat , El Husseiny Noha M. , Morad Mohamed A.
Published: March 2023

Introduction

Telemedicine (TM) is a method for follow-up of patients experiencing chronic morbidities, such as those with myeloproliferative neoplasms (MPNs) and thalassemia, particularly during the coronavirus disease 2019 (COVID-19) pandemic.

Objective

To assess medical services administrated to patients with MPNs and thalassemia after 6 months of online follow-up.

Patients and methods

During the COVID-19 pandemic, of 250 patients who were regularly following up in the hematology clinic, only 45 patients with MPNs and 30 patients with thalassemia were able to connect through the WhatsApp group. Six months later, a questionnaire was given to patients. We asked if COVID-19 affected their routine medical service and receipt of their treatment. Finally, did they attend the clinic despite TM service and why?

Results

Two patient groups were included. A total of 75 patients were included, comprising 56 (80%) females and 19 (20%) males. The median age was 51.2 (16–73) years in the MPN group and 29.5 (17–50) years in the thalassemia group. All patients with MPNs found the application easy to use, whereas 93.3% of patients with thalassemia found the application to be easy. TM consultations increased since the start of online follow-up for 41 patients, with no change in three and decreased in one patient. Seven patients had suspicious COVID symptoms, and four of them notified the consultant. During the follow-up, about 205 consultations and 300 laboratory results had been medically debated through WhatsApp groups or audio-visual calls.

Conclusion

Evidence supports that TM is a fast, safe, and effective tool to increase care and decrease morbidity for both patients with MPNs and adult patients with thalassemia during the pandemic time.
Melioidosis with Amoebic Coinfection Presenting as Multifocal Liver and Splenic Abscess
Authors: Aravind R. , Premkumar Dinu Abirami , Gopal Lokeshwari , Varghese Joy
Published: March 2023

We present a case of chronic calcific pancreatitis with diabetes mellitus who presented with multifocal liver and splenic abscesses which were found to be a coinfection of Entamoeba histolytica and Burkholderia pseudomallei. The existence of concurrent infection by two such unrelated organisms provides an example of the enigma of infections in areas of decreased host resistance.
The Burden of Occult Hepatitis B Virus Infection in Hepatocellular Carcinoma in Nigeria
Authors: Egboh Stella-Maris Chinma , Davwar Pantong Mark
Published: March 2023

The WHO guideline advocates for hepatitis B surface antigen (HBsAg) as the initial diagnostic test for hepatitis B virus (HBV) infection. There are, however, patients who may have the persistence of viral DNA in the liver or blood despite having undetectable HBsAg; these patients also need to be accounted for, especially in endemic areas. These groups of patients are also at risk of developing hepatocellular carcinoma, and they need to be identified. We advocate for funding to support projects and research centers that are able to detect occult HBV infection. This will be valuable in the reduction of the occult transmission of HBV and reactivation of quiescent HBV.
The Management of Walled off Pancreatic Necrosis in 2023 – Lessons Learned From the Past Two Decades
Authors: Paramasivan Piramanayagam
Published: March 2023

Acute necrotizing pancreatitis is associated with the formation of acute necrotic collection which eventually becomes encapsulated to form walled-off pancreatic necrosis (WOPN) in 4 weeks. Good quality randomized controlled trials published in the past two decades have helped us understand and refine the management of WOPN. This review summarizes the lessons learned from these trials. Sterile pancreatic necrosis is managed conservatively unless they cause symptoms. Infected pancreatic necrosis leads to organ failure and mortality. The treatment paradigm for infected pancreatic necrosis has shifted from early open necrosectomy to initial intensive medical management, followed by interventions. Step-up approach with initial drainage followed by minimally invasive necrosectomy, in selected patients who do not improve with drainage, has been shown to have better short-term and long-term outcomes. Endoscopic step-up approach has been associated with a lower incidence of pancreaticocutaneous fistula and similar mortality or organ failure as compared to surgical step-up approach. Dedicated endoscopic accessories for performing necrosectomy are undergoing validation studies, which hopefully will help perform necrosectomy with less complications.
Crystalline lens decentration and tilt in eyes with different axial lengths and their associated factors
Authors: Shen Lin , Yang Wenli , Li Dongjun , Wang Ziyang , Chen Wei , Zhao Qi , Li Yifeng , Cui Rui , Liu Qian
Published: March 2023

Purpose:

To analyze crystalline lens decentration and tilt in eyes with different axial lengths (ALs) using a swept-source anterior segment optical coherence tomography (SS-AS-OCT).

Methods:

Patients with normal right eyes who visited our hospital between December 2020 and January 2021 were included in this cross-sectional study. Data on crystalline lens decentration and tilt, AL, aqueous depth (AD), central corneal thickness (CCT), lens thickness (LT), lens vault (LV), anterior chamber width (ACW), and angle κ were collected.

Results:

A total of 252 patients were included and divided into normal (n = 82), medium–long (n = 89), and long (n = 81) AL groups. The average age of these patients was 43.63 ± 17.02 years. The crystalline lens decentration (0.16 ± 0.08, 0.16 ± 0.09, and 0.20 ± 0.09 mm, P = 0.009) and tilt (4.58° ± 1.42°, 4.06° ± 1.32°, and 2.84° ± 1.19°, P < 0.001) were significantly different among the normal, medium, and long AL groups. Crystalline lens decentration was correlated with AL (r = 0.466, P = 0.004), AD (r = 0.358, P = 0.006), ACW (r = −0.004, P = 0.020), LT (r = −0.141, P = 0.013), and LV (r = −0.371, P = 0.003). Crystalline lens tilt was correlated with age (r = 0.312, P < 0.001), AL (r = −0.592, P < 0.001), AD (r = −0.436, P < 0.001), ACW (r = −0.018, P = 0.004), LT (r = 0.216, P = 0.001), and LV (r = 0.311, P = 0.003).

Conclusion:

Crystalline lens decentration was positively correlated with AL, and tilt was negatively correlated with AL.