bluuebunny/crossref_metadata_2025 huggingface dataset:
ОГЛЯД КНИГИ А. КРАВЦОВОЇ «МАМО, ДАВАЙ ПОВЕРНЕМО ЙОГО ЛЕЛЕЦІ! АБО ДОПОМАГАЄМО ДИТИНІ ПОДРУЖИТИСЯ З БРАТИКОМ ЧИ СЕСТРИЧКОЮ»
Authors: Мірошник Зоя
Published: January 2024

У книзі Анни Кравцової «Мамо, давай повернемо його лелеці! або Допомагаємо дитині подружитися з братиком чи сестричкою» представлено унікальну психологічну подорож сучасних дітей у власні родини та занурення в дитячо-батьківські взаємини. У поданій статті-рецензії розкрито психологічні особливості дитячих ревнощів, які народжуються й розвиваються в ситуації появи другої дитини. Авторка статті-рецензії зазначає, що формування вміння в малюків власного стилю спілкування не лише з батьками, а й з братами і сестрами впливає на прояви як позитивних, так і негативних емоцій. Ця книга, безумовно, сприяє кращому розумінню батьками особливостей особистості своїх малюків, а також допомагає вчитися сучасним батькам розкривати сімейні конфлікти та знаходити шляхи їх вирішення. Авторка стисло описує наповнення кожного з дев’яти розділів від моменту, коли «Родина чекає ще на одну дитину» (перший розділ) до «Дитячих ревнощів і дитячої творчості» (дев’ятий розділ) і вказує на труднощі, з якими стикаються батьки і діти на кожному із цих етапів. У кожному з розділів Анною Кравцовою досліджено дотичність дитячого психоаналізу до теорії прив’язаності та психології розвитку. Зрештою, формулюється висновок, що дитячі ревнощі – це складне людське почуття, яке спостерігається не лише в дорослих стосунках, а і в стосунках батьки – діти. Матеріали дуже цікаві своєю складністю й розмаїттям стилів і підходів: від глибокого наукового аналізу до яскравих висловлювань і віршів дітей; бесід з мамами дітей різних вікових категорій, що переживають ревнощі; від сильних почуттів у описі випадків до логічних заключень та узагальнень психологічних і психаналітичних теорій і концепцій. Розділи книги являють собою єдину систему ідей, думок, наукового й життєвого досвіду автора, взаємодіючи й доповнюючи одне одного.
СУЧАСНА ПРОПАГАНДА ТА ПРОПАГАНДИСТСЬКІ СТАНИ ДУШІ: ПСИХОАНАЛІТИЧНА ПЕРСПЕКТИВА
Authors: Романов Ігор
Published: January 2024

У статті представлено огляд психоаналітичних досліджень пропаганди, її зв’язків з індивідуальними та груповими проявами механізмів заперечення, зворотної дії, проєктивної та інтроєктивної ідентифікацій. Окреслюються предметні сфери та здобутки психоаналітичних досліджень пропаганди в період Першої та Другої світових війн: різниця демократичних і тоталітарних форм пропаганди та лідерства як зсув з его до суперцентрованої позиції; еволюція західної пропаганди в напрямі більш раціональних і менш моралістичних та емоційних дискурсів; принципова відмінність цілей психоаналізу та пропаганди на індивідуальному, суспільному і внутрішньо психічному рівні. На цьому тлі ставиться питання про зворотні процеси в сучасній пропаганді, крайнім виразом якої виступає російська пропаганда. Її риси досліджуються порівняно з класичними зразками пропаганди ХХ сторіччя, обґрунтовується її специфічна поліформність, відсутність жорсткої прив’язки до систематизованої ідеології. Незважаючи на спорідненість російської пропаганди з деякими рисами сучасної західної, вбудована в неї модель взаємодії людини та суспільства споріднює її з класичними тоталітарними формами. Пропонується поняття «пропагандистських станів душі», яке описує внутрішню психічну констеляцію, що підкоряє функціювання его деструктивній частині самості, що спотворює мислення й перевірку реальності. Це робить особливо актуальним антипропагандистський потенціал психоаналізу як на індивідуальному, так і на соціальному рівні. Ця теза набуває додаткового значення у світлі гіпотези про пропаганду як наслідок неспроможності скорботи й нездатності переробляти колективний травматичний досвід, включно із втратою минулих форм колективної ідентичності.
Working paper on the numerical modelling framework to compare different accounting schemes
Authors: Paschen Marius , Meier Felix , Rickels Wilfried
Published: January 2024

Any integration of extra carbon dioxide removal (CDR) via terrestrial or marine sink enhancement into climate policies requires accounting for their effectiveness in reducing atmospheric carbon concentration. Different accounting methods have been introduced to quantify the impacts of sink enhancements. Here, we provide a manual for the different accounting methods, accompanying the implementation of the accounting methods in a R-file which is free for download. Hence, the material allows applying the different accounting ethods and for demonstration purposes we provide a numerical example.
LEADERSHIP DEVELOPMENT PROGRAM: THE CORRELATION BETWEEN TRAINING DESIGN AND TRAINING TRANSFER BASED ON THE ISLAMIC PERSPECTIVE
Authors: Mohd Zainol Noor Azmi , Mohamed Hasan Al-Banna , Yasin Redwan
Published: January 2024

Training management literature highlighted that the leadership development training design consists of two important features:training content and training method. The ability of the management to appropriately implement such program features may lead to enhanced training transfer in the organizations. Reviews of the literature on leadership development found that the literature is mostly dominated by Western scholar perspectives. Although this relationship has been studied in the Islamic management views, the leadership development training design only contributed little explanation of workplace training programs. Most Islamic scholars in this field of study only discussed conceptually without suggesting a framework that can serve as a guide for the subsequent measurement. Therefore, this study was undertaken to determine the relationship between leadership development trainingdesign and training transfer using questionnaires with 412 leaders in the Malaysian Army Infantry Corps. The path model of SmartPLSversion 3.2.5 results stated that there is a significant relationship between the training content and training transfer, and there is asignificant relationship between the training method and training transfer. These outcomes showed two important findings. Firstly, the relationship between training content significantly correlated with training transfer. Secondly, the relationship between training methods significantly correlated with training transfer. Statistically, this result demonstrates that the leadership development training design acts as an important antecedent of training transfer in organizations. Further, the discussions, implications and conclusion are elaborated.
Embedded: The Interface of the Physical, Virtual, and Social
Authors: Bickley Andrea
Published: January 2024

The unprecedented access to and abundance of media in contemporary society has changed how we experience and perceive our surroundings and, by extension, architecture by subjecting us to fragmented realities. This increasingly virtual world raises issues of perception, spectatorship, and desire. Meanwhile, the physical and virtual spaces traditionally associated with socialization, entertainment, work, and sleep overlap, intertwine, and intermingle creating hybrid spaces. We are embedded within a system of hybridized virtual and physical spaces, and this system becomes increasingly manipulative and difficult to escape as it penetrates the most intimate and vulnerable of spaces–the bed. However, the tensions arising from the hybridized space of the bed in an increasingly virtual world can reveal new opportunities for inhabiting spaces with others. The bed can reorient space or reinforce the status quo. This thesis, therefore, presents a speculative future inviting us to be critical of our present.
The Architecture of Tranquility: the Calming Affect of Modern Form
Authors: Rubenzahl Dan
Published: January 2024

Architecture has the ability to create a tranquil environment which, through the perception of form, instills a calming feeling. The fundamental principles of Modernism, influenced by traditional Japanese aesthetics, were expressive of a form-follows-function approach, which integrates essential form into nature. This synthesis remains effective as a formal catalyst which enables a sense of calm. The Functionalist divergence from original Modernism resulted in an oversimplification of form which was perceived as cold and sterile in its expression. In reaction to this extreme, Post-Modernism rejected simplicity for its own sake and opted instead for form of additive complexity which embraced formal contradictions. Neither of these two approaches enable a feeling of calm because of an extraneous imbalance which is perceived and instills a sense of disequilibrium. A balance between the two poles, however, in which form and function are unified in harmony, may enable a feeling of calm through essence.
The Digital Craftsman
Authors: Toscano Keegan
Published: January 2024

Craftsmanship within the context of contemporary architectural design has failed to play a meaningful role, confined by nostalgic perceptions. Likewise, the architect has also forgone this territory of design and making due to the dependencies of abstract processes, severing the response of a material-based approach. As recognized, craftsmanship serves to provide this necessary dialogue between the processes of making and its translation into an expressive, material-based architecture. The digital craftsman re-evaluates this dialogical understanding by adapting some of the architects’ contemporary tools in developing a new design methodology towards architectural craftsmanship. 

This thesis examines various facets of the craftsman’s process, strategically situating a distinctive design approach, evaluated through a series of design-based investigations illustrating the manifested nuances of craftsmanship in architecture. This novel perspective offers the architect a valuable means of responding to the notion of craftsmanship as a valid form of design and making within our contemporary architectural discourse.
Prevalence of errors in the preparation and administration of intravenous drugs in adults: Meta-analysis with meta-regression
Authors: Figueredo Irlane Batista , Passos Silvia da Silva Santos , Opitz Simone Perufo , Castro Caroline Tianeze de , Santos Djanilson Barbosa dos
Published: January 2024

Objective: To evaluate the average prevalence of errors in the preparation and administration of intravenous medications in a hospital by means of a meta-analysis. Method: Systematic review through meta-analysis with meta-regression, registered in PROSPERO (CRD42022324431), with a search in the seven databases, using the Rayyan QCRY®. The methodological quality of the selected studies was assessed using the Newcastle-Ottawa Scale. The meta-analysis was calculated using the random-effect model and adjusted by the inverse of the variance, and analyses were carried out to investigate heterogeneity. Results: 34 primary studies were included. The estimated prevalence of errors in the preparation and administration of intravenous drugs was 41,23% (IC95% 30,51–51,96; I2 = 100,00%). Conclusion: The results reflect the lack of health systems official data on the reporting of errors in institutions, the basis for the effective strategies that ensure for patient safety in the process medicated.
Ventilação mecânica como estratégia protetora nos pacientes com SDRA: Uma revisão integrativa
Authors: Lima Cayo Raphael da Rocha , Santos Elenildo Aquino dos
Published: January 2024

Introdução: A denominação “ventilação mecânica” (VM) aponta uma série de técnicas que substitui a função ventilatória dos pulmões, tem como principal objetivo dar suporte no tratamento de pacientes acometidos com insuficiência respiratória aguda ou crônica agudizada, substituindo total ou parcialmente a ventilação espontânea, configurando-se uma das formas de abordar o paciente com SDRA, porém, quando regida na forma convencional, pode acometer o pulmão, potencializando um processo inflamatório no mesmo, e como prevenção, usa-se a estratégia protetora. Objetivo: descrever as principais evidências científicas atuais sobre VM protetora no paciente com SDRA. Metodologia: Trata-se de uma revisão integrativa da literatura. A busca dos estudos primários ocorreu nas bases de dados: PubMed e Scielo. Resultados: Foram encontrados 35 artigos dentro da estratégia de busca estabelecida por meio dos descritores Síndrome do Desconforto Respiratório, Lesão Pulmonar, e Proteção. Destes, 5 se encaixaram com a temática da pesquisa. Conclusão: O ∆P é o principal preditor de mortalidade na SDRA.
Eficácia da pimavanserina para pacientes com psicose na doença de Parkinson: Uma revisão sistemática
Authors: Mendes Rayanne da Cunha , Souza Sâmara Pesqueira , Silva Junior Luis Claudio Almeida da , Carvalho Jordy Silva de , Coelho Victor Hugo Oliveira Martins , Horta Wagner Gonçalves
Published: January 2024

O presente estudo tem como objetivo avaliar a eficácia da pimavanserina em pacientes com psicose na doença de Parkinson. Foram avaliados seus efeitos no quadro clínico da psicose, assim como suas reações adversas e influências na qualidade de vida dos pacientes. Trata-se de uma Revisão Sistemática da Literatura de natureza qualitativa, com estratégia de busca os Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) e avaliação metodológica Critical Appraisal Skill Programme (CASP), analisando-se ao total 8 artigos. Os artigos foram pesquisados nas bases de dados PubMed, excluindo revisões simples de literatura e artigos incompletos. Foram incluídos os publicados nos últimos 10 anos (2013-2023) e disponibilizados em inglês. Após uma análise sistemática dos estudos, conclui-se que a pimavanserina demonstra eficácia na redução dos sintomas alucinógenos e delirantes. Além disso, constatou-se que a combinação da pimavanserina com outros medicamentos, especialmente a clozapina, se mostrou promissora em termos de segurança, principalmente na melhora das funções cognitivas. Os pacientes apresentaram melhorias na qualidade do sono e vigília, além de redução significativa dos sintomas. Ademais, entre os efeitos colaterais estão pneumonia, episódios de síncope, doença pulmonar obstrutiva, embolia pulmonar e trombose venosa profunda. Espera-se que estudos futuros continuem a desenvolver mais pesquisas de intervenção sobre a eficácia da medicação no tratamento da doença de Parkinson, destacando benefícios e melhorias na qualidade de vida dos pacientes através do uso do fármaco na prática médica.
Public beliefs and perceptions related to ecofascism
Authors: Gareiou Z , Giannarou S , Drimili E , Vatikiotis L , Zervas E
Published: January 2024

The concept of ecofascism describes the distortion of ecology for the purpose of gaining greater and wider audience, popularising ideologies and fulfilling xenophobic and nationalistic goals by regimes such as the far-right agenda and the radical ecological groups. This study investigates the issue of ecofascism in Europe, using the example of Greece, by examining the views of the citizens of Greece on the links between the political parties and ecology and environment. A survey of 600 people was conducted in Greece, using questionnaires on ecofascism, politics and ecological issues. The survey showed that the sample has, in general, humanistic, democratic and quite left-wing ideals. However, some of the respondents indicated nationalistic, even fascist, trends. Because the current socio-economic and political conditions in Europe can fuel an outbreak of fascism, we should be very cautious about these findings, due to the growing impact of far-right ideas on society. Some aspects of the ecofascist rhetoric still continue to mislead, revealing the sly and worrying penetration of fascism in Greek society on the pretext of the protection of the Greek environment.
Levels of BDNF and NGF in Adolescent Rat Hippocampus Neonatally Exposed to Methamphetamine Along With Environmental Alterations
Authors: ČECHOVÁ B , MIHALČÍKOVÁ L , VACULIN Š , ŠANDERA Š , ŠLAMBEROVÁ R
Published: January 2024

Neurotrophins are proteins included in development and functioning of various processed in mammalian organisms. They are important in early development but as well as during adulthood. Brain – derived neurotrophic factor (BDNF) and nerve growth factor (NGF) have been previously linked with many psychiatric disorders such as depression and addiction. Since during postnatal development, brain undergoes various functional and anatomical changes, we included preweaning environment enrichment (EE), since enrichment has been linked with improved function and development of the several brain structure such as hippocampus (HP), in which we monitored these changes. On the other hand, social isolation has been linked with depression and anxiety-like behavior, therefore postweaning social isolation has been added to this model as well and animal were exposed to this condition till adolescence. We examined if all these three factors had impact on BDNF and NGF levels during three phases of adolescence – postnatal days (PDs) 28, 35 and 45. Our results show that EE did not increase BDNF levels neither in control or MA exposed animals and these results are similar for both direct and indirect exposure. On the other side, social separation after weaning did reduce BDNF levels in comparison to standard housing animals but this effect was reversed by direct MA exposure. In terms of NGF, EE environment increased its levels only in indirectly exposed controls and MA animals during late adolescence. On the other hand, social separation increased NGF levels in majority of animals.
PEMBERDAYAAN MASYARAKAT DESA RANOEYA MELALUI PELATIHAN PEMBUATAN CEMILAN CHURROS DAUN SALAM (SYZYGIUM POLYANTHUM WIGHT) BAGI PENDERITA DIABETES DI KECAMATAN WAWOTOBI KABUPATEN KONAWE
Authors: Musdalipah Musdalipah , Nunigrum Aisyah , Daud Nur Saadah , Hikma Eny Nur , Rusli Nirwati , Badia Esti , Reymon  , Wulaisfan Randa , Fauziah Yulianti , Karmilah  , Yodha Agung Wibawa Mahatva , Alam Syamsu
Published: January 2024

Salam leaf is one of the herbal plants that contains several compounds that effectively reduce blood glucose levels, namely saponins, tannins, phenolics and flavonoids. Empirically, the leaves of salamka are used by the Ranoeya Village community as an additional ingredient to add flavor to dishes. Community service activities of LPPM Politeknik Bina Husada and Halu Oleo University as a community empowerment in the context of utilizing bay leaves as snacks for diabetics in Wawotobi District. The potential development of churros snacks as an alternative food has the opportunity to be a very good snack for health that can be consumed by all ages. The method of the activity was carried out by training the making of churros to the Ranoeya village community of 25 people. The activity started from harvesting, wet sorting, dry sorting, drying, cutting and pulverizing bay leaves. Bay leaves were prepared with various concentrations, namely FI (1 g), FII (3 g), and FIII (5 g) and organoleptic evaluation was carried out including color, odor, taste and shape of the preparation. The results showed that Ranoeya villagers were very enthusiastic in participating in this activity. Organoleptic test results showed churros in formula FI were yellow, FII were greenish yellow and FIII were brownish green. The aroma test showed FI, FII and FIII had a distinctive aroma of milk vanilla flavor. The results of the taste test on F1 churros taste in general. F2 has a slightly concentrated bay leaf flavor, F3 has a concentrated bay leaf flavor. The taste test on F1 has the taste of churros in general, F2 is slightly concentrated, and F3 is concentrated. Hardness test on F1, F2 and F3 have good texture. The favorability test showed churros in F1 (90%), B (7%), C (3%). The results showed that Salam leaves can be formulated in the form of churros snacks and can be utilized as a healthy snack for diabetics in Ranoeya Village, Wawotobi District, Konawe Regency
FORMULASI DAN UJI STABILITAS FISIK SEDIAAN MASKER WAJAH GEL PEEL-OFF EKSTRAK KULIT BUAH NAGA MERAH (Hylocereus polyrhizus)
Authors: Aditama Agnis Pondineka Ria , Kusumaningtyas Rili , Karimah Widya Nuratika , Paramita Denok Risky Ayu , Rashati Dewi , Muslikh Faisal Akhmal
Published: January 2024

Red dragon fruit (Hylocereus polyrhizus) has many benefits. The fruit peel is rich in antioxidants which have the potential to improve the health and beauty of facial skin. Traditional use requires a long preparation time and formulation is needed to make preparations with more effective, practical and long-lasting storage. The facial mask formulation is a peel-off gel preparation that can moisturize the skin and increase the effect of the active ingredients. Quality assurance, namely safety, quality and benefits, is very necessary, which can be done by testing the physical stability of the preparation. The aim of this research is to obtain a formula made from the active ingredient Hylocereus polyrhizus fruit peel extract in the form of a peel-off gel facial mask and the best physical stability. HPMC was used as a carrier base by varying the concentration, namely 2%; 3% and 5%. Evaluation of the physical stability of the preparation was carried out, namely homogeneity, organoleptic, spreadability, viscosity, pH and dry time tests of 14 and 28 days. Storage is carried out at warm (30-40°C), room (15-30°C), and cold (2-8°C) temperatures. The best formula is 3% HPMC concentration with the best physical stability. Variations in storage temperature have an effect on physical organoleptics (color), viscosity and dispersion, but have no effect on physical homogeneity, drying time and pH. Cold temperatures (2-8°C) show the best stability because they can maintain physical stability.
PLANE KINEMATICS IN LORENTZIAN HOMOTHETIC MULTIPLICATIVE CALCULUS
Authors: Es Hasan
Published: January 2024

In this study, Lorentzian plane homothetic multiplicative calculus kinematics is discussed.
Lorentzian plane homothetic multiplicative calculus movement, the pole points of a point X relative to
the moving and fixed plane are discussed. In this motion, the velocities and accelerations of a point
X are obtained. In this motion, the relations between the velocities and accelerations of a point X are
obtained. In addition, new theorems and results are given.
A desterritorialização lésbica na visibilidade submarina de filmes brasileiros contemporâneos
Authors: De Almeida Ana Caroline
Published: January 2024

Este artigo propõe investigar como quatro curtas-metragens brasileiros realizados entre 2019 e 2021, com protagonistas/es lésbicas, produzem formas fílmicas que, tal como o mar, criam desterritórios onde não é possível estabelecer fronteiras fixas e separabilidades entre as pessoas e sua afetação no mundo e com o mundo. Um espaço onde a densidade do fundo do oceano transforma os corpos filmados em transbordamento. Os filmes em questão são Quebramar (2019), de Cris Lyra; Bege euforia (2021), de Anália Alencar; A felicidade delas (2019), de Carol Rodrigues e Uma paciência selvagem me trouxe até aqui (2021), de Érica Sarmet. Juntos, eles testemunham um cinema brasileiro lésbico contemporâneo que não apenas desafia noções pré-estabelecidas de uma imaginação cisheteronormativa do que deve ser o corpo lésbico, como tensionam o constante regime de in/visibilidade da lésbica no cinema (BRANDÃO, SOUSA, 2019) usando, com frequência, um devir-mar em suas estratégias audiovisuais.
A crítica a domesticidade nos filmes de Chantal Akerman: Saute ma ville (1968) e Jeanne Dielman, 23 Quai du Commerce, 1080 Bruxelles (1975)
Authors: Marchiori da Silva Natalia
Published: January 2024

Nossa hipótese é que por meio da claustrofobia doméstica que emana de toda a estrutura dos filmes Saute ma Ville e Jeanne Dielman, 23 Quai du Commerce, 1080 Bruxelles, tanto na temática como na estilística, as críticas a domesticidade e ao confinamento das mulheres se fazem presentes. Assim sendo, o cinema apresenta-se como uma linguagem importante para a visibilização de pautas e agendas feministas, que no caso dos filmes analisados possuem convergências com os movimentos de segunda onda. Tal característica é um traço marcado na produção de Chantal Akerman, sobretudo nos filmes realizados até o fim da década de 1970.
Multi-scale in silico and ex silico mechanics of 3D printed cochlear implants for local drug delivery
Authors: Isaakidou A. , Ganjian M. , van Hoften R. , Saldivar M. C. , Leeflang M. A. , Groetsch A. , Wątroba M. , Schwiedrzik J. , Mirzaali M. J. , Apachitei I. , Fratila-Apachitei L. E. , Zadpoor A. A.
Published: January 2024

The currently available treatments for inner ear disorders often involve systemic drug administration, leading to suboptimal drug concentrations and side effects. Cochlear implants offer a potential solution by providing localized and sustained drug delivery to the cochlea. While the mechanical characterization of both the implants and their constituent material is crucial to ensure functional performance and structural integrity during implantation, this aspect has been mostly overlooked. This study proposes a novel methodology for the mechanical characterization of our recently developed cochlear implant design, namely, rectangular and cylindrical, fabricated using two-photon polymerization (2 PP) with a novel photosensitive resin (IP-Q™). We used in silico computational models and ex silico experiments to study the mechanics of our newly designed implants when subjected to torsion mimicking the foreseeable implantation procedure. Torsion testing on the actual-sized implants was not feasible due to their small size (0.6 × 0.6 × 2.4 mm³). Therefore, scaled-up rectangular cochlear implants (5 × 5 × 20 mm³, 10 × 10 × 40 mm³, and 20 × 20 × 80 mm³) were fabricated using stereolithography and subjected to torsion testing. Finite element analysis (FEA) accurately represented the linear behavior observed in the torsion experiments. We then used the validated Finite element analysis models to study the mechanical behavior of real-sized implants fabricated from the IP-Q resin. Mechanical characterization of both implant designs, with different inner porous structures (pore size: 20 μm and 60 μm) and a hollow version, revealed that the cylindrical implants exhibited approximately three times higher stiffness and mechanical strength as compared to the rectangular ones. The influence of the pore sizes on the mechanical behavior of these implant designs was found to be small. Based on these findings, the cylindrical design, regardless of the pore size, is recommended for further research and development efforts.
Laser-light cueing shoes with integrated foot pressure and inertial sensing for investigating the impact of visual cueing on gait characteristics in Parkinson’s disease individuals
Authors: Chan Hsiao-Lung , Chen Rou-Shayn , Kuo Cheng-Chung , Chen Yi-Tao , Liaw Jiunn-Woei , Liao Guo-Sheng , Lin Wan-Ting , Chien Shih-Hsun , Chang Ya-Ju
Published: January 2024

Gait disorders are a fundamental challenge in Parkinson’s disease (PD). The use of laser-light visual cues emitted from shoes has demonstrated effective in improving freezing of gait within less restrictive environments. However, the effectiveness of shoes-based laser-light cueing may vary among individuals with PD who have different types of impairments. We introduced an innovative laser-light visual shoes system capable of producing alternating visual cues for the left and right feet through one-side cueing at a time, while simultaneously recording foot inertial data and foot pressures. The effects of this visual cueing system on gait patterns were assessed in individuals with PD, both those with well-gait and those with worse-gait. Our device successfully quantified gait characteristics, including the asymmetry in the center of pressure trajectory, in individuals with PD. Furthermore, visual cueing prolonged stride times and increased the percentage of stance phase, while concurrently reducing stride length in PD individuals with well-gait. Conversely, in PD individuals with worse-gait, visual cueing resulted in a decreased freeze index and a reduction in the proportion of intervals prone to freezing episodes. The effects of visual cueing varied between PD individuals with well-gait and those with worse-gait. Visual cueing slowed down gait in the well-gait group while it appeared to mitigate freezing episodes in worse-gait group. Future researches, including enhancements to extend the projection distance of visual cues and clinical assessments conducted in real-world settings, will help establish the clinical utility of our proposed visual cueing system.
Fecal bacteria-free filtrate transplantation is proved as an effective way for the recovery of radiation-induced individuals in mice
Authors: Zhang Hang , Dong Miaomiao , Zheng Jixia , Yang Yapeng , He Jinhui , Liu Tianhao , Wei Hong
Published: January 2024

Background

Ionizing radiation can cause intestinal microecological dysbiosis, resulting in changes in the composition and function of gut microbiota. Altered gut microbiota is closely related to the development and progression of radiation-induced intestinal damage. Although microbiota-oriented therapeutic options such as fecal microbiota transplantation (FMT) have shown some efficacy in treating radiation toxicity, safety concerns endure. Therefore, fecal bacteria-free filtrate transplantation (FFT), which has the potential to become a possible alternative therapy, is well worth investigating. Herein, we performed FFT in a mouse model of radiation exposure and monitored its effects on radiation damage phenotypes, gut microbiota, and metabolomic profiles to assess the effectiveness of FFT as an alternative therapy to FMT safety concerns.

Results

FFT treatment conferred radioprotection against radiation-induced toxicity, representing as better intestinal integrity, robust proinflammatory and anti-inflammatory cytokines homeostasis, and accompanied by significant shifts in gut microbiome. The bacterial compartment of recipients following FFT was characterized by an enrichment of radioprotective microorganisms (members of family Lachnospiraceae). Furthermore, metabolome data revealed increased levels of microbially generated short-chain fatty acids (SCFAs) in the feces of FFT mice.

Conclusions

FFT improves radiation-induced intestinal microecological dysbiosis by reshaping intestinal mucosal barrier function, gut microbiota configurations, and host metabolic profiles, highlighting FFT regimen as a promising safe alternative therapy for FMT is effective in the treatment of radiation intestinal injury.
Bacteriophage therapy for drug-resistant Staphylococcus aureus infections
Authors: Liu Kaixin , Wang Chao , Zhou Xudong , Guo Xudong , Yang Yi , Liu Wanying , Zhao Rongtao , Song Hongbin
Published: January 2024

Drug-resistant Staphylococcus aureus stands as a prominent pathogen in nosocomial and community-acquired infections, capable of inciting various infections at different sites in patients. This includes Staphylococcus aureus bacteremia (SaB), which exhibits a severe infection frequently associated with significant mortality rate of approximately 25%. In the absence of better alternative therapies, antibiotics is still the main approach for treating infections. However, excessive use of antibiotics has, in turn, led to an increase in antimicrobial resistance. Hence, it is imperative that new strategies are developed to control drug-resistant S. aureus infections. Bacteriophages are viruses with the ability to infect bacteria. Bacteriophages, were used to treat bacterial infections before the advent of antibiotics, but were subsequently replaced by antibiotics due to limited theoretical understanding and inefficient preparation processes at the time. Recently, phages have attracted the attention of many researchers again because of the serious problem of antibiotic resistance. This article provides a comprehensive overview of phage biology, animal models, diverse clinical case treatments, and clinical trials in the context of drug-resistant S. aureus phage therapy. It also assesses the strengths and limitations of phage therapy and outlines the future prospects and research directions. This review is expected to offer valuable insights for researchers engaged in phage-based treatments for drug-resistant S. aureus infections.
PlasmaBlade-assisted surgical septal myectomy: technique and our experience
Authors: Garg Pankaj , Lykins Amy , Alomari Mohammad , Reynolds Jordan P. , Johnson Elizabeth , Sareyyupoglu Basar
Published: January 2024

Background

The pulsed-electron avalanche knife (PEAK) PlasmaBlade provides an atraumatic, scalpel-like cutting precision and electrocautery-like hemostasis. PlasmaBlade operates near body temperature, and its long, thin, and malleable tip can overcome the limitations of a surgical knife. In this study, we aimed to evaluate our clinical experience and histopathological outcomes of septal myectomy using PlasmaBlade.

Methods

Electronic medical records were reviewed for preoperative, operative, and follow-up data of the patients who underwent septal myectomy using PEAK PlasmaBlade at our institute between January 2019 and December 2022. Histopathology of the myectomy specimens was reviewed for the depth of muscle necrosis and compared with the left atrial appendage (LAA) specimen.

Results

Twenty-nine patients underwent septal myectomy using the PEAK PlasmaBlade. No mortality was reported. The mean age was 60.6 ± 12.5 years, and 58.6% of patients were male. Peak left ventricular outflow tract (LVOT) gradients were 40.5 ± 34.9 mmHg at rest and 56.5 ± 34.9 mmHg after provocation. Concomitant procedures performed were LAA ligation in 20 (69.0%), aortic valve replacement in 5 (17.2%), and coronary artery bypass grafting in 3 (10.3%) patients. Postoperative complications were complete heart block in one (3.4%) and ventricular septal defect in two (6.9%) patients. Both the ventricular septal defects were identified intraoperatively and repaired. Histopathology of myectomy specimens demonstrated cautery artifact limited to <50 µm depth compared to >1,000 µm with conventional electrocautery. At a mean follow-up of 8.4 ± 10.3 months, the mean LVOT gradient was 4.4 ± 5.8 mmHg at rest and 9.5 ± 3.3 mmHg after provocation. All patients were alive and in New York Heart Association class I/II. No patient developed complications or required reintervention or reoperation.

Conclusion

Adequate septal myectomy can be precisely and safely performed using the PEAK PlasmaBlade with minimal collateral damage.
Association of different cell types and inflammation in early acne vulgaris
Authors: Huang Lei , Yang Shuyun , Yu Xiuqin , Fang Fumin , Zhu Liping , Wang Lu , Zhang Xiaoping , Yang Changzhi , Qian Qihong , Zhu Tingting
Published: January 2024

Acne vulgaris, one of the most common skin diseases, is a chronic cutaneous inflammation of the upper pilosebaceous unit (PSU) with complex pathogenesis. Inflammation plays a central role in the pathogenesis of acne vulgaris. During the inflammatory process, the innate and adaptive immune systems are coordinately activated to induce immune responses. Understanding the infiltration and cytokine secretion of differential cells in acne lesions, especially in the early stages of inflammation, will provide an insight into the pathogenesis of acne. The purpose of this review is to synthesize the association of different cell types with inflammation in early acne vulgaris and provide a comprehensive understanding of skin inflammation and immune responses.
Progressions of the correlation between lipid metabolism and immune infiltration characteristics in gastric cancer and identification of BCHE as a potential biomarker
Authors: Wang Shibo , Huang Xiaojuan , Zhao Shufen , Lv Jing , Li Yi , Wang Shasha , Guo Jing , Wang Yan , Wang Rui , Zhang Mengqi , Qiu Wensheng
Published: January 2024

Background

Globally, gastric cancer (GC) is a category of prevalent malignant tumors. Its high occurrence and fatality rates represent a severe threat to public health. According to recent research, lipid metabolism (LM) reprogramming impacts immune cells’ ordinary function and is critical for the onset and development of cancer. Consequently, the article conducted a sophisticated bioinformatics analysis to explore the potential connection between LM and GC.

Methods

We first undertook a differential analysis of the TCGA queue to recognize lipid metabolism-related genes (LRGs) that are differentially expressed. Subsequently, we utilized the LASSO and Cox regression analyses to create a predictive signature and validated it with the GSE15459 cohort. Furthermore, we examined somatic mutations, immune checkpoints, tumor immune dysfunction and exclusion (TIDE), and drug sensitivity analyses to forecast the signature’s immunotherapy responses.

Results

Kaplan-Meier (K-M) curves exhibited considerably longer OS and PFS (p<0.001) of the low-risk (LR) group. PCA analysis and ROC curves evaluated the model’s predictive efficacy. Additionally, GSEA analysis demonstrated that a multitude of carcinogenic and matrix-related pathways were much in the high-risk (HR) group. We then developed a nomogram to enhance its clinical practicality, and we quantitatively analyzed tumor-infiltrating immune cells (TIICs) using the CIBERSORT and ssGSEA algorithms. The low-risk group has a lower likelihood of immune escape and more effective in chemotherapy and immunotherapy. Eventually, we selected BCHE as a potential biomarker for further research and validated its expression. Next, we conducted a series of cell experiments (including CCK-8 assay, Colony formation assay, wound healing assay and Transwell assays) to prove the impact of BCHE on gastric cancer biological behavior.

Discussion

Our research illustrated the possible consequences of lipid metabolism in GC, and we identified BCHE as a potential therapeutic target for GC. The LRG-based signature could independently forecast the outcome of GC patients and guide personalized therapy.
Evolution of guanylate binding protein genes shows a remarkable variability within bats (Chiroptera)
Authors: Pinheiro Ana , Borges J. Ricardo , Côrte-Real João Vasco , Esteves Pedro J.
Published: January 2024

Background

GBPs (guanylate binding proteins), an evolutionary ancient protein family, play a key role in the host’s innate immune response against bacterial, parasitic and viral infections. In Humans, seven GBP genes have been described (GBP1-7). Despite the interest these proteins have received over the last years, evolutionary studies have only been performed in primates, Tupaia and rodents. These have shown a pattern of gene gain and loss in each family, indicative of the birth-and-death evolution process.

Results

In this study, we analysed the evolution of this gene cluster in several bat species, belonging to the Yangochiroptera and Yinpterochiroptera sub-orders. Detailed analysis shows a conserved synteny and a gene expansion and loss history. Phylogenetic analysis showed that bats have GBPs 1,2 and 4-6. GBP2 has been lost in several bat families, being present only in Hipposideidae and Pteropodidae. GBPs1, 4 and 5 are present mostly as single-copy genes in all families but have suffered duplication events, particularly in Myotis myotis and Eptesicus fuscus. Most interestingly, we demonstrate that GBP6 duplicated in a Chiroptera ancestor species originating two genes, which we named GBP6a and GBP6b, with different subsequent evolutionary histories. GBP6a underwent several duplication events in all families while GBP6b is present as a single copy gene and has been lost in Pteropodidae, Miniopteridae and Desmodus rotundus, a Phyllostomidae. With 14 and 15 GBP genes, Myotis myotis and Eptesicus fuscus stand out as having far more copies than all other studied bat species. Antagonistically, Pteropodidae have the lowest number of GBP genes in bats.

Conclusion

Bats are important reservoirs of viruses, many of which have become zoonotic diseases in the last decades. Further functional studies on bats GBPs will help elucidate their function, evolutionary history, and the role of bats as virus reservoirs.
Contribution of aged organic carbon to suspended particulate organic carbon in the western equatorial Indian Ocean
Authors: Kang Sujin , Zhang Hailong , Ding Yang , Zhao Meixun , Son Yeong Baek , Son Purna , Rho Tae Keun , Kang Dong-Jin
Published: January 2024

We investigated dual carbon isotopes within the vertical water column at sites 67-1 and 67-2 of the western equatorial Indian Ocean to determine the source and age of particulate organic carbon (POC) and thus evaluated the contributions of modern and fossil (aged) POC. The concentration of POC ranged from 7 to 47.3 μgC L−1, δ13CPOC values ranged from –31.8 to –24.4‰, and Δ14CPOC values ranged from –548 to –111‰. Higher values of δ13CPOC and Δ14CPOC near the surface indicated an influence of autochthonous POC, whereas decreasing trends toward the bottom suggested a contribution of aged OC sources to the total POC pool. The contribution of fossil POC was lower near the surface, accounting for only 12% and 6% of the total POC at sites 67-1 and 67-2, respectively; however, in the deeper layers below 1,000 m, the contribution of fossil POC increased to 52% and 44% of the total POC at the two sites. Mechanisms for the increased contributions of fossil OC within deeper POC include the inflow of aged OC from sediments resuspended near slopes, the adsorption of old dissolved organic carbon in deep water masses, and the impact of aged OC that may originate from hydrothermal sources. This study highlights the importance of aged OC in the carbon cycle of the equatorial Indian Ocean.
Unlocking the potential of big data and AI in medicine: insights from biobanking
Authors: Akyüz Kaya , Cano Abadía Mónica , Goisauf Melanie , Mayrhofer Michaela Th.
Published: January 2024

Big data and artificial intelligence are key elements in the medical field as they are expected to improve accuracy and efficiency in diagnosis and treatment, particularly in identifying biomedically relevant patterns, facilitating progress towards individually tailored preventative and therapeutic interventions. These applications belong to current research practice that is data-intensive. While the combination of imaging, pathological, genomic, and clinical data is needed to train algorithms to realize the full potential of these technologies, biobanks often serve as crucial infrastructures for data-sharing and data flows. In this paper, we argue that the ‘data turn’ in the life sciences has increasingly re-structured major infrastructures, which often were created for biological samples and associated data, as predominantly data infrastructures. These have evolved and diversified over time in terms of tackling relevant issues such as harmonization and standardization, but also consent practices and risk assessment. In line with the datafication, an increased use of AI-based technologies marks the current developments at the forefront of the big data research in life science and medicine that engender new issues and concerns along with opportunities. At a time when secure health data environments, such as European Health Data Space, are in the making, we argue that such meta-infrastructures can benefit both from the experience and evolution of biobanking, but also the current state of affairs in AI in medicine, regarding good governance, the social aspects and practices, as well as critical thinking about data practices, which can contribute to trustworthiness of such meta-infrastructures.
Depression-associated gut microbes, metabolites and clinical trials
Authors: Wang Meiling , Song Zhaoqi , Lai Shirong , Tang Furong , Dou Lijun , Yang Fenglong
Published: January 2024

Depression is one of the most prevalent mental disorders today. Over the past decade, there has been considerable attention given to the field of gut microbiota associated with depression. A substantial body of research indicates a bidirectional communication pathway between gut microbiota and the brain. In this review, we extensively detail the correlation between gut microbiota, including Lactobacillus acidophilus and Bifidobacterium longum, and metabolites such as short-chain fatty acids (SCFAs) and 5-hydroxytryptamine (5-HT) concerning depression. Furthermore, we delve into the potential health benefits of microbiome-targeted therapies, encompassing probiotics, prebiotics, and synbiotics, in alleviating depression. Lastly, we underscore the importance of employing a constraint-based modeling framework in the era of systems medicine to contextualize metabolomic measurements and integrate multi-omics data. This approach can offer valuable insights into the complex metabolic host-microbiota interactions, enabling personalized recommendations for potential biomarkers, novel drugs, and treatments for depression.
Iron uptake pathway of Escherichia coli as an entry route for peptide nucleic acids conjugated with a siderophore mimic
Authors: Tsylents Uladzislava , Burmistrz Michał , Wojciechowska Monika , Stępień Jan , Maj Piotr , Trylska Joanna
Published: January 2024

Bacteria secrete various iron-chelators (siderophores), which scavenge Fe3+ from the environment, bind it with high affinity, and retrieve it inside the cell. After the Fe3+ uptake, bacteria extract the soluble iron(II) from the siderophore. Ferric siderophores are transported inside the cell via the TonB-dependent receptor system. Importantly, siderophore uptake paths have been also used by sideromycins, natural antibiotics. Our goal is to hijack the transport system for hydroxamate-type siderophores to deliver peptide nucleic acid oligomers into Escherichia coli cells. As siderophore mimics we designed and synthesized linear and cyclic Nδ-acetyl-Nδ-hydroxy-l-ornithine based peptides. Using circular dichroism spectroscopy, we found that iron(III) is coordinated by the linear trimer with hydroxamate groups but not by the cyclic peptide. The internal flexibility of the linear siderophore oxygen atoms and their interactions with Fe3+ were confirmed by all-atom molecular dynamics simulations. Using flow cytometry we found that the designed hydroxamate trimer transports PNA oligomers inside the E. coli cells. Growth recovery assays on various E. coli mutants suggest the pathway of this transport through the FhuE outer-membrane receptor, which is responsible for the uptake of the natural iron chelator, ferric-coprogen. This pathway also involves the FhuD periplasmic binding protein. Docking of the siderophores to the FhuE and FhuD receptor structures showed that binding of the hydroxamate trimer is energetically favorable corroborating the experimentally suggested uptake path. Therefore, this siderophore mimic, as well as its conjugate with PNA, is most probably internalized through the hydroxamate pathway.