bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Analysis of NLP-Based Models for Company Classification
Authors: Rizinski Maryan , Jankov Andrej , Sankaradas Vignesh , Pinsky Eugene , Mishkovski Igor , Trajanov Dimitar
Published: January 2024

The task of company classification is traditionally performed using established standards, such as the Global Industry Classification Standard (GICS). However, these approaches heavily rely on laborious manual efforts by domain experts, resulting in slow, costly, and vendor-specific assignments. Therefore, we investigate recent natural language processing (NLP) advancements to automate the company classification process. In particular, we employ and evaluate various NLP-based models, including zero-shot learning, One-vs-Rest classification, multi-class classifiers, and ChatGPT-aided classification. We conduct a comprehensive comparison among these models to assess their effectiveness in the company classification task. The evaluation uses the Wharton Research Data Services (WRDS) dataset, consisting of textual descriptions of publicly traded companies. Our findings reveal that the RoBERTa and One-vs-Rest classifiers surpass the other methods, achieving F1 scores of 0.81 and 0.80 on the WRDS dataset, respectively. These results demonstrate that deep learning algorithms offer the potential to automate, standardize, and continuously update classification systems in an efficient and cost-effective way. In addition, we introduce several improvements to the multi-class classification techniques: (1) in the zero-shot methodology, we use TF-IDF to enhance sector representation, yielding improved accuracy in comparison to standard zero-shot classifiers; (2) next, we use ChatGPT for dataset generation, revealing potential in scenarios where datasets of company descriptions are lacking; and (3) we also employ K-Fold to reduce noise in the WRDS dataset, followed by conducting experiments to assess the impact of noise reduction on the company classification results.
Industry 4.0 Innovation: A Systematic Literature Review on the Role of Blockchain Technology in Creating Smart and Sustainable Manufacturing Facilities
Authors: Alazab Moutaz , Alhyari Salah
Published: January 2024

Industry 4.0 has revolutionized manufacturing processes and facilities through the creation of smart and sustainable production facilities. Blockchain technology (BCT) has emerged as an invaluable asset within Industrial Revolution 4.0 (IR4.0), offering increased transparency, security, and traceability across supply chains. This systematic literature review explores the role of BCT in creating smart and sustainable manufacturing facilities, while exploring its implications for supply chain management (SCM). Through a detailed examination of 82 research articles, this review highlights three areas where BCT can have a dramatic effect on smart and sustainable manufacturing: firstly, BCT can promote green production methods by supporting efficient resource use, waste reduction strategies and eco-friendly production methods; and secondly, it allows companies to implement smart and eco-friendly manufacturing practices through BCT solutions. BCT promotes intelligent manufacturing systems by facilitating real-time data sharing, predictive maintenance, and automated decision-making. Furthermore, BCT strengthens SCM by increasing visibility, traceability, and collaboration between partners of SC operations. The review also highlights the potential limitations of BCT, such as scalability challenges and the need for standardized protocols. Future research should focus on addressing these limitations and further exploring the potential of BCT in IR4.0.
One Year Outcomes Following Transplantation with COVID-19-Positive Donor Hearts: A National Database Cohort Study
Authors: Wolfe Stanley B. , Singh Ruby , Paneitz Dane C. , Rabi Seyed Alireza , Chukwudi Chijioke C. , Asija Richa , Michel Eriberto , Ganapathi Asvin M. , Osho Asishana A.
Published: January 2024

The current understanding of the safety of heart transplantation from COVID-19+ donors is uncertain. Preliminary studies suggest that heart transplants from these donors may be feasible. We analyzed 1-year outcomes in COVID-19+ donor heart recipients using 1:3 propensity matching. The OPTN database was queried for adult heart transplant recipients between 1 January 2020 and 30 September 2022. COVID-19+ donors were defined as those who tested positive on NATs or antigen tests within 21 days prior to procurement. Multiorgan transplants, retransplants, donors without COVID-19 testing, and recipients allocated under the old heart allocation system were excluded. A total of 7211 heart transplant recipients met the inclusion criteria, including 316 COVID-19+ donor heart recipients. Further, 290 COVID-19+ donor heart recipients were matched to 870 COVID-19− donor heart recipients. Survival was similar between the groups at 30 days (p = 0.46), 6 months (p = 0.17), and 1 year (p = 0.07). Recipients from COVID-19+ donors in the matched cohort were less likely to experience postoperative acute rejection prior to discharge (p = 0.01). National COVID-19+ donor heart usage varied by region: region 11 transplanted the most COVID-19+ hearts (15.8%), and region 6 transplanted the fewest (3.2%). Our findings indicate that COVID-19+ heart transplantation can be performed with safe early outcomes. Further analyses are needed to determine if long-term outcomes are equivalent between groups.
Instability of Healthy Overweight and Obesity Phenotypes over the Long Term in Young Participants in the HARVEST Study: Influence of Sex
Authors: Palatini Paolo , Saladini Francesca , Mos Lucio , Vriz Olga , Ermolao Andrea , Battista Francesca , Mazzer Adriano , Canevari Mattia , Rattazzi Marcello
Published: January 2024

Background: Whether healthy metabolic status is stable or only temporary is still controversial. The aim of the present study was to determine the frequency of the transition from metabolically healthy to metabolically unhealthy status, or vice versa, over the long term. Methods: We examined 970 individuals of 18 to 45 years of age. The participants’ mean age was 33.1 ± 8.6 years and mean BP was 145.5 ± 10.6/93.5 ± 5.7 mmHg. Participants were classified into four groups according to whether they had normal weight or overweight/obesity (OwOb) and were metabolically healthy or unhealthy. After 7.5 years, 24.3% of men and 41.9% of women in the metabolically healthy normal-weight group remained metabolically healthy (p < 0.0001). Among the metabolically healthy OwOb participants, 31.9% remained metabolically healthy, with a similar frequency in men and women. However, more OwOb women (19.1%) than men (5.7%) achieved normal weight (p < 0.0001). Among the metabolically unhealthy OwOb subjects, 81.8% of men and 69.3% of women remained metabolically unhealthy, 7.4% of men and 12.0% of women transitioned to OwOb healthy status, and 10.7% of men and 18.7% of women achieved normal weight (men versus women, p < 0.0001). Predictors of transition to unhealthy status were high BP, high BMI, and smoking. Male sex was a borderline predictor of progression to unhealthy status in OwOb participants (p = 0.073). Conclusion: These data show that metabolically healthy status is a highly unstable condition in both normal-weight and OwOb individuals. The impairment of metabolic status was more frequent in men than in women. Lifestyle counseling produced beneficial effects in almost one-third of metabolically unhealthy OwOb women and in less than one-fifth of men.
Importance of Comprehensive Assessment in Brugada Syndrome. Reply to Kataoka, N.; Imamura, T. How to Diagnose and Risk Stratify Brugada Syndrome. Comment on “Matusik et al. Twelve-Lead ECG, Holter Monitoring Parameters, and Genetic Testing in Brugada Syndrome: Insights from Analysis of Multigenerational Family with a History of Sudden Cardiac Arrest during Physical Activity. J. Clin. Med. 2023, 12, 6581”
Authors: Matusik Paweł T. , Bijak Piotr , Kaźnica-Wiatr Magdalena , Karpiński Marek , Matusik Patrycja S. , Maziarz Andrzej , Podolec Piotr , Lelakowski Jacek
Published: January 2024

We would like to thank Dr. Imamura for their interest in our study and their valuable comments on diagnostics and risk stratification in Brugada syndrome (BrS) [...]
Partial Stent-in-Stent Method with an Uncovered Self-Expandable Metallic Stent for Unresectable Malignant Hilar Bile Duct Obstruction
Authors: Shimosaka Takuya , Takeda Yohei , Yamashita Taro , Seki Yuta , Kawahara Shiho , Hirai Takayuki , Suto Noriyuki , Sakamoto Yuri , Hamamoto Wataru , Koda Hiroki , Onoyama Takumi , Matsumoto Kazuya , Yashima Kazuo , Isomoto Hajime , Yamaguchi Naoyuki
Published: January 2024

(1) Background: There is controversy regarding stent placement for unresectable malignant hilar biliary obstruction (UMHBO). We mainly use the partial stent-in-stent (PSIS) method with an uncovered self-expandable metallic stent (UCSEMS) based on the drainage area and patency period. In this study, we investigated the usefulness and safety of the PSIS method. (2) Methods: In total, 59 patients who underwent the PSIS method for UMHBO at our hospital were included in the study. The technical success rate, clinical success rate, time to recurrent biliary obstruction (TRBO) and overall survival (OS) from the first placement, factors affecting TRBO and OS, and early complications within 30 days after the procedure were evaluated retrospectively. (3) Results: The technical and clinical success rates were 100% and 96.6%, respectively, with a TRBO of 121 days [95% confidence interval: 82–231] and an OS of 194 days [95% confidence interval: 113–305] after the first placement. Early complications occurred in nine patients (15.3%), including five cases of cholangitis, three cases of pancreatitis, and one case of cholecystitis. (4) Conclusions: The PSIS method for UMHBO is safe and useful with high technical and clinical success rates.
Seminal Oxidative Stress and Sperm DNA Fragmentation in Men from Couples with Infertility or Unexplained Recurrent Pregnancy Loss
Authors: Rasmussen Johanne Mejlholm Kold , Dalgaard Maya Isabella Riise , Alipour Hiva , Dardmeh Fereshteh , Christiansen Ole Bjarne
Published: January 2024

(1) Background: This case–control study examined whether men from couples with unexplained recurrent pregnancy loss (RPL) or infertility exhibited higher seminal oxidative stress (OS) and sperm DNA fragmentation (SDF) compared to fertile controls. (2) Methods: The study included 30 participants from each group: unexplained RPL, unexplained infertility, and proven fertility. Data were collected at Aalborg University Hospital tertiary RPL and fertility treatment clinics (Aalborg, Denmark), excluding couples with mixed conditions for homogeneity. Semen samples were analyzed using computer-aided sperm analysis (CASA) for concentration, motility, and morphology. SDF was assessed via a CASA-based sperm chromatin dispersion test. OS was measured as static oxidation-reduction potential (sORP). (3) Results: The results showed no significant OS differences between groups. The RPL group had significantly lower SDF levels than the control group. A significant positive correlation between SDF and OS was observed in the infertility group. Overall, this study did not find significant differences in OS levels between men from couples with unexplained RPL or infertility and fertile controls, while SDF levels were lower in the RPL group compared to controls. (4) Conclusion: In conclusion, despite the existing literature suggesting that OS and SDF are negative prognostic factors, our findings suggest they may not be reliable diagnostic markers for RPL and infertility.
Alternate Stable States Theory: Critical Evaluation and Relevance to Marine Conservation
Authors: Guarini Jean-Marc , Coston-Guarini Jennifer
Published: January 2024

In their 2023 book, “The Blue Compendium: From Knowledge to Action for a Sustainable Ocean Economy”, Lubchenko and Haugan invoked alternate stable (AS) states marginally as an undesired consequence of sources of disturbance on populations, communities and ecosystems. They did not provide detailed arguments, but considered the existence of AS states as a given. Conversely, May, in his 1977 Nature article, pointed out that, when applied to systems that are complex, “the [AS states] theory remains largely metaphorical”. This is the starting point of this critical review, which aims to re-examine the general theory behind AS states in ecological systems and its applications to marine ecology and conservation. The focus is first on theory, taking as examples communities that sustain competition and studying the relative importance of the fluxes of individuals between simple low-dimension, interconnected systems. We find that a minimal formulation of fluxes is sufficient to obtain a set of non-null multiple stable (MS) states and to trigger shifts between AS states when fluxes become large enough. This provides new insights into the theory of rescue and mass effects by distinguishing them through a threshold at which the system dynamics shift from one stable equilibrium to another. Then, we consider how the theoretical framework of AS states has been applied in marine environments. It appears that many applications have confounded shifts between AS states and changes in the structure of systems, particularly when the complexity of the systems increases. The main difficulty for any application remains that the concepts of MS and AS states can only be established and validated for low-dimension systems and simplified experiments. This is because the mathematical properties of models that describe large-dimension, complex systems deviate from the observed characteristics of their real-world counterparts. There are many intriguing scientific challenges around the plausible shifts between AS states, but a deeper understanding and characterization of their occurrence in nature would require a significant investment in modeling to formulate predictive ecosystem models.
Classification and Spatial Differentiation of Subdistrict Units for Sustainable Urban Renewal in Megacities: A Case Study of Chengdu
Authors: Zuo Jin , Fan Jiahui , Huang Xingyu , Li Chen , Luo Jiancheng
Published: January 2024

Sustainable urban renewal is an important approach to achieving high-quality urban development. The elements of megacities are diverse, and their structures are complex. It is critical to carry out the scientific classification of grassroots governance units based on the concept and needs of urban renewal to promote targeted sustainability evaluation and achieve the precise application of renewal design and planning. This study takes the jurisdiction of Chengdu City as an example and constructs a hierarchical dimension composite classification. For this classification, 128 grassroots governance units are divided into nine types, according to their obvious spatial differences. Based on the properties of these types, suggestions for evaluating and implementing urban renewal are proposed: (1) high-density central areas generally face the dilemma of complex and rigid needs and administrative weaknesses, so the development of public participatory governance is an urgent issue; (2) in transitional suburban zones, areas on and between the development axes are significantly different, indicating that extra attention should be paid to the fairness of the renewal of semi-urbanized areas; (3) outer areas are generally marginalized in urban renewal processes and destructive redevelopment behaviors should be avoided.
Key Drivers of Land Use Changes in the Rural Area of Gargano (South Italy) and Their Implications for the Local Sustainable Development
Authors: Esengulova Nazgul , Balena Pasquale , De Lucia Caterina , Lopolito Antonio , Pazienza Pasquale
Published: January 2024

This study examines the dynamics of land use and land cover change (LULCC) in the Gargano area (Southern Italy) to reveal crucial insights into the socio-economic and environmental impacts on its unique natural and cultural resources. This analysis was conducted using a mixed approach of GIS data and expert interviews to investigate significant changes in the Gargano area, from 2000 to 2018, and their drivers. Artificial surfaces gained 22% of their original surfaces, while heterogeneous areas and pastures lost 25% and 78%, respectively. Urbanization and deforestation emerged as major concerns, reflecting heightened sensitivity to these transformative processes. Agricultural intensification and support policies were perceived as potential pressure sources on specific natural components. Conversely, these drivers counteracted land abandonment. Drivers such as education level and agricultural extensification were seen as levers for a more desirable land cover dynamic. Identified actions include providing targeted support for agriculture within environmental constraints, addressing land ownership fragmentation, supporting agricultural extensification, and promoting environmental awareness.
Occurrence of Pseudomonas syringae pvs. actinidiae, actinidifoliorum and Other P. syringae Strains on Kiwifruit in Northern Spain
Authors: González Ana J. , Díaz David , Ciordia Marta , Landeras Elena
Published: January 2024

Pseudomonas syringae pv. actinidiae (Psa), the agent causing bacterial canker of kiwifruit, has been present in the Principality of Asturias (PA), Northern Spain, since 2013, although with restricted distribution. In this study, 53 strains collected in kiwifruit orchards in PA during the period 2014–2020 were characterized by a polyphasic approach including biochemical and phylogenetic analysis. Thirty-three strains, previously identified by PCR as Psa, have been found to be a homogeneous group in phylogenetic analysis, which seems to indicate that there have been few introductions of the pathogen into the region. Two strains were confirmed as P. syringae pv. actinidifoliorum (Pfm), so this is the first report of Pfm in the PA. The remaining 18 strains were found to be close to P. avellanae and P. syringae pv. antirrhini or to strains described as Pfm look-alikes. Pathogenicity tests carried out on peppers with a selection of strains have shown that both Psa and Pfm caused clear damage, while the 18 atypical strains caused variable lesions. It would be necessary to carry out pathogenicity testing of atypical strains on kiwifruit plants to study the role of these strains in the kiwifruit pathosystem to evaluate their pathogenic potential in this crop.
Impact Analysis of Photoperiodic Disorder on the Eyestalk of Chinese Mitten Crab (Eriocheir sinensis) through High-Throughput Sequencing Technology
Authors: Zhang Baoli , Chai Yuqiao , Xu Yingkai , Huang Ziwei , Hu Xueqing , Li Yingdong
Published: January 2024

Light is an indispensable factor in the healthy growth of living organisms, and alterations in the photoperiod can have consequences for body homeostasis. The eyestalk is a photosensitive organ that secretes various hormones to regulate the Chinese mitten crab (Eriocheir sinensis). However, the photoperiod-dependent eyestalk patterns of gene expression that may underlie changes in body homeostasis are unknown. In this study, we investigated the molecular mechanisms involved in eyestalk transcriptomic responses in E. sinensis under different photoperiod regimes on days 2, 4, and 6. The photoperiods tested were 12, 24, and 0 h light/day. In total, we obtained 110, 958, 348 clean datasets and detected 1809 differentially expressed genes (DEGs). Genes involved in the crustacean hyperglycemic hormone superfamily and juvenile hormones were observed, which play important roles in gonadal development, growth, and immunity in E. sinensis and may also be involved in photoperiod adaptation. In addition, the MAPK signaling pathway was the only signaling pathway identified in the continuous light group but was absent in the continuous darkness group. We suggest that the MAPK pathway is highly responsive to light input during the subjective night and insensitive to light during the middle of the subjective day. These results provide insight into the molecular mechanisms underlying the effects of photoperiod on the immune regulation of E. sinensis.
Adsorption of Heavy Metals on Alkali-Activated Zeolite Foams
Authors: Svobodová Eliška , Tišler Zdeněk , Peroutková Kateřina , Strejcová Kateřina , Abrham Jan , Šimek Josef
Published: January 2024

Elevated concentrations of heavy metals in natural waters can cause significant ecological problems. It is therefore essential to ensure their removal from any water discharged into the environment immediately, especially in case of an accident, where there is a risk of releasing large quantities or high concentrations. The aim of this paper is to test a newly developed adsorbent for the removal of heavy metals from aqueous solutions—in particular, it is very fast adsorption, and thus efficiency, during clean-ups. The alkali-activated foamed zeolite adsorbent was laboratory-prepared and -tested in both batch and flow-through arrangements on single and multi-component solutions and compared with natural zeolite. The experimental setup for batch adsorption consisted of a set of samples and solutions containing iron, cobalt, manganese, zinc and nickel. The samples were put on a horizontal shaker with a 500 mg adsorbent loading in a 50 mL solution. The column adsorption experimental setup consisted of a glass column with an inside diameter of 15 mm and a bed length of 165 mm. A measured amount of each adsorbent was added to the column to achieve a filter fixed-bed height of 160 mm. The high efficiency of the tested adsorbent on various heavy metals was confirmed. The adsorbent has a high potential for use in decontamination processes, water protection and landscape revitalization. Due to its rapid precipitation and subsequent fixation of metal cations in the form of insoluble oxide or hydroxide, it can be used as an emergency adsorbent, the great advantage of which is its low production cost and natural origin.
Classification of Real Solutions of the Fourth Painlevé Equation
Authors: Schiff Jeremy , Twiton Michael
Published: January 2024

Painlevé transcendents are usually considered as complex functions of a complex variable, but, in applications, it is often the real cases that are of interest. We propose a classification of the real solutions of Painlevé IV (or, more precisely, symmetric Painlevé IV) using a sequence of symbols describing their asymptotic behavior and singularities. We give rules for the sequences that are allowed (depending on the parameter values). We look in detail at the existence of globally nonsingular real solutions and determine the dimensions of the spaces of such solutions with different asymptotic behaviors. We show that for a generic choice of the parameters, there exists a unique finite sequence of singularities for which Painlevé IV has a two-parameter family of solutions with this singularity sequence. There also exist solutions with singly infinite and doubly infinite sequences of singularities, and we identify which such sequences are possible. We look at the singularity sequences (and asymptotics) of special solutions of Painlevé IV. We also show two other results concerning special solutions: rational solutions can be obtained as the solution of a set of polynomial equations, and other special solutions can be obtained as the solution of a first-order differential equation.
Ocular Complications after COVID-19 Vaccination: A Systematic Review
Authors: Hurissi Eman A. , Abuallut Ismail I. , Dibaji Mohammed Qassem , Jaly Abdulaziz , Alhazmi Abdulaziz H. , Abuageelah Bandar M. , Alameer Khalid M. , Alyami Yousef M.
Published: January 2024

Background and Objectives: The COVID-19 pandemic affects various populations worldwide. The discovery of vaccinations was necessary for the prevention and elimination of the disease. Despite the high importance of these vaccinations, they may cause some complications, such as ocular complications. This study aims to draw attention to the possible complications of the vaccination and highlight its importance. Materials and Methods: Systematic review of the literature from January 2021 to January 2023. A total of 20 published articles were included and reported cases of ocular complications in patients who received COVID-19 vaccines. Results: A total of 243 patients with verified ocular complications following the COVID-19 vaccination were included, ranging in age from 18 to 84 years. The most common ocular complications reported in the current study were ocular inflammatory complications, which represented 47.3%, followed by optic neuritis (24.3%). Retinal artery occlusion, retinal vein occlusion, acute macular neuroretinopathy, and paracentral acute middle maculopathy represented 10.7%. Herpetic ocular infections and herpetic eye disease (14%). Nearly half (42%) of the patients with ocular problems received the Pfizer-BioNTech vaccination. Conclusions: Despite the high importance of the COVID-19 vaccination, it was found that it is associated with the occurrence of some ocular complications. Future projects should come with more extensive prospective studies to further elucidate the underlying mechanisms and risk factors associated with ocular complications following COVID-19 vaccination, thereby enhancing our understanding and guiding appropriate management strategies.
Construction and Application of an Electrochemical Sensor for Determination of D-Penicillamine Based on Modified Carbon Paste Electrode
Authors: Mohammadnavaz Arefeh , Beitollahi Hadi , Modiri Sina
Published: January 2024

D-penicillamine (D-PA) is a sulfur-containing drug that has been used for various health conditions. However, like any medication, overdosing on D-PA can have adverse effects and may require additional treatment. Therefore, developing simple and sensitive methods for sensing D-PA can play a crucial role in improving its efficacy and reducing its side effects. Sensing technologies, such as electrochemical sensors, can enable accurate and real-time measurement of D-PA concentrations. In this work, we developed a novel electrochemical sensor for detecting D-PA by modifying a carbon paste electrode (CPE) with a multi-walled carbon nanotube-Co3O4 nanocomposite, benzoyl-ferrocene (BF), and ionic liquid (IL) (MWCNT-Co3O4/BF/ILCPE). Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and chronoamperometry (CHA) were employed to explore the electrochemical response of D-PA on the developed sensor, the results of which verified a commendable electrochemical performance towards D-PA. Under optimized conditions, the developed sensor demonstrated a rapid response to D-PA with a linear dynamic range of 0.05 μM–100.0 μM, a low detection limit of 0.015 μM, and a considerable sensitivity of 0.179 μA μM−1. Also, the repeatability, stability, and reproducibility of the MWCNT-Co3O4/BF/ILCPE sensor were studied and showed good characteristics. In addition, the detection of D-PA in pharmaceutical and biological matrices yielded satisfactory recoveries and relative standard deviation (RSD) values.
A New Compact Wideband Filter Based on a Coupled Stepped Impedance Resonator
Authors: Sheta Abdel-Fattah A. , Elshafiey Ibrahim
Published: January 2024

A new compact wideband filter is introduced to address the requirements of recent communication and radar systems. The filter is based on a quarter-wavelength short-circuit coupled stepped impedance resonator (SIR). The analytical solution shows that the suggested SIR resonator provides a compact size and a wide stopband response, which are essential features in many wireless communication systems. The analytical results also reveal that increasing the impedance ratio of the SIR extends the stopband by increasing the first spurious response and reducing its total length. A compact two-coupled short-circuit SIR filter is designed at 1.23 GHz. The design approach is validated using an ideal transmission line modeling analysis and electromagnetic simulation using CST Microwave Studio 2021. The proposed structure is shown to be flexible, allowing the achievement of a relative bandwidth as low as 5% and as high as 50%. A four-resonator filter is designed by cascading two stages of the designed two-coupled short-circuit SIR filter, which are coupled through a quarter-wavelength line. The simulation results illustrate that the suggested structure can be used to design a filter with any number of resonators. The filter is implemented using a high-resolution LPKF laser machine on Rogers RT/duroid 6010.2LM material with a thickness of 0.635 mm. From the measurements, the bandwidth is found to be 390 MHz and centered at 1325 MHz (29.4% relative bandwidth) and the insertion loss is 1.3 dB. The simulation and experimental results verify the proposed approach and indicate the potential of this component in meeting the design requirements of next-generation microwave circuits related to flexibility and size-compactness.
Novel Weft-Knitted Strain Sensors for Motion Capture
Authors: Fischer Susanne , Abtahi Bahareh , Warncke Mareen , Böhmer Carola , Winger Hans , Sachse Carmen , Mersch Johannes , Häntzsche Eric , Nocke Andreas , Cherif Chokri
Published: January 2024

Functional electrical stimulation (FES) aims to improve the gait pattern in cases of weak foot dorsiflexion (foot lifter weakness) and, therefore, increase the liveability of people suffering from chronic diseases of the central nervous system, e.g., multiple sclerosis. One important component of FES is the detection of the knee angle in order to enable the situational triggering of dorsiflexion in the right gait phase by electrical impulses. This paper presents an alternative approach to sensors for motion capture in the form of weft-knitted strain sensors. The use of textile-based strain sensors instead of conventional strain gauges offers the major advantage of direct integration during the knitting process and therefore a very discreet integration into garments. This in turn contributes to the fact that the FES system can be implemented in the form of functional leggings that are suitable for inconspicuous daily use without disturbing the wearer unnecessarily. Different designs of the weft-knitted strain sensor and the influence on its measurement behavior were investigated. The designs differed in terms of the integration direction of the sensor (wale- or course-wise) and the width of the sensor (number of loops) in a weft-knitted textile structure.
Performance Improvement of In-Ga-Zn Oxide Thin-Film Transistors by Excimer Laser Annealing
Authors: Zhang Xiaohui , Li Yaping , Li Yanwei , Xie Xinwang , Yin Longhai
Published: January 2024

We applied excimer laser annealing (ELA) on indium-zinc oxide (IZO) and IZO/indium-gallium-zinc oxide (IGZO) heterojunction thin-film transistors (TFTs) to improve their electrical characteristics. The IZO and IZO/IGZO heterojunction thin films were prepared by the physical vapor deposition method without any other annealing process. The crystalline state and composition of the as-deposited film and the excimer-laser-annealed films were analyzed by X-ray diffraction and X-ray photoelectron spectroscopy. In order to further enhance the electrical performance of TFT, we constructed a dual-heterojunction TFT structure. The results showed that the field-effect mobility could be improved to 9.8 cm2/V·s. Surprisingly, the device also possessed good optical stability. The electron accumulation at the a-IZO/HfO, HfO/a-IGZO, and a-IGZO/gate insulator (GI) interfaces confirmed the a-IGZO-channel conduction. The dual-heterojunction TFT with IZO/HfO/a-IGZO-assisted ELA provides a guideline for overcoming the trade-off between high mobility (μ) and positive VTh control for stable enhancement mode operation with increased ID.
Driving Principle and Stability Analysis of Vertical Comb-Drive Actuator for Scanning Micromirrors
Authors: Shan Yameng , Qian Lei , Wang Junduo , Wang Kewei , Zhou Peng , Li Wenchao , Shen Wenjiang
Published: January 2024

We have developed a manufacturing process for micromirrors based on microelectromechanical systems (MEMS) technology. The process involves designing an electrostatic vertically comb-driven actuator and utilizing a self-alignment process to produce a height difference between the movable comb structure and the fixed comb structure of the micromirror. To improve the stability of the micromirror, we propose four instability models in micromirror operation with the quasi-static driving principle and structure of the micromirror considered, which can provide a basic guarantee for the performance of vertical comb actuators. This analysis pinpoints factors leading to instability, including the left and right gap of the movable comb, the torsion beams of the micromirror, and the comb-to-beams distance. Ultimately, the voltages at which device failure occurs can be determined. We successfully fabricated a one-dimensional micromirror featuring a 0.8 mm mirror diameter and a 30 μm device layer thickness. The height difference between the movable and fixed comb structures was 10 μm. The micromirror was able to achieve a static mechanical angle of 2.25° with 60 V@DC. Stable operation was observed at voltages below 60 V, in close agreement with the theoretical calculations and simulations. At the driving voltage of 80 V, we observed the longitudinal displacement movement of the comb fingers. Furthermore, at a voltage of 129 V, comb adhesion occurred, resulting in device failure. This failure voltage corresponds to the lateral torsional failure voltage.
Early Circulating Edema Factor in Inhalational Anthrax Infection: Does It Matter?
Authors: Tessier Emilie , Cheutin Laurence , Garnier Annabelle , Vigne Clarisse , Tournier Jean-Nicolas , Rougeaux Clémence
Published: January 2024

Anthrax toxins are critical virulence factors of Bacillus anthracis and Bacillus cereus strains that cause anthrax-like disease, composed of a common binding factor, the protective antigen (PA), and two enzymatic proteins, lethal factor (LF) and edema factor (EF). While PA is required for endocytosis and activity of EF and LF, several studies showed that these enzymatic factors disseminate within the body in the absence of PA after intranasal infection. In an effort to understand the impact of EF in the absence of PA, we used a fluorescent EF chimera to facilitate the study of endocytosis in different cell lines. Unexpectedly, EF was found inside cells in the absence of PA and showed a pole-dependent endocytosis. However, looking at enzymatic activity, PA was still required for EF to induce an increase in intracellular cAMP levels. Interestingly, the sequential delivery of EF and then PA rescued the rise in cAMP levels, indicating that PA and EF may functionally associate during intracellular trafficking, as well as it did at the cell surface. Our data shed new light on EF trafficking and the potential location of PA and EF association for optimal cytosolic delivery.
Silicas with Covalently Anchored Fluorosolvatochromic Dyes Suitable for Naked-Eye Detection of Colourless Compounds
Authors: Navrátilová Tereza , Havlík Martin , Tatar Ameneh , Hricková Karolína , Dolenský Bohumil
Published: January 2024

In this work, two stilbene derivatives with different substituents on the phenolic core (phenyl and dimethoxyphenyl) were prepared. The fluorosolvatochromic response of their N-propylated derivatives was studied in a solution of twelve different solvents using UV–Vis absorption and fluorescence emission spectra. Both stilbazolium dyes showed a significant negative solvatochromic effect, with a hypsochromic shift in the visible absorption band of approximately 232 nm and 265 nm for phenyl and the dimethoxyphenyl derivative, respectively, when the solvent was changed from water to pyridine. The stilbene derivatives were subsequently N-alkylated with (3-iodopropyl)trimethoxysilane and covalently anchored to the silica surface. The fluorosolvatochromic response of the prepared silicas compared to N-propylated dyes was then evaluated colorimetrically under daylight and UV illumination. The fluorosolvatochromic behaviour of the anchored dyes was preserved on the silica surface; therefore, the modified silicas could be used for the visual detection of colourless liquids.
Development and Analysis of Silver Nitroprusside Nanoparticle-Incorporated Sodium Alginate Films for Banana Browning Prevention
Authors: Zhang Lina , Sathiyaseelan Anbazhagan , Zhang Xin , Lu Yuting , Wang Myeong-Hyeon
Published: January 2024

Banana (Musa acuminate) has been popular among consumers worldwide due to its rich nutrients and minerals. However, bananas are highly susceptible to the physical and biological factors that lead to postharvest loss during transportation and storage. In this work, novel sodium alginate (SA) films incorporated with silver nitroprusside nanoparticles (AgNNPs) were prepared to extend the shelf life of bananas through antibacterial and antioxidant coating. The results exhibited that AgNNPs were cubical and that their size was <500 nm, with metal composition being Ag and Fe. Additionally, the incorporation of AgNNPs in the SA film was seen in FE-SEM and zeta analysis, with an average size of about 365.6 nm. Furthermore, the functional and crystalline properties of AgNNPs were assessed through FTIR and XRD. Transmittance testing of the SA-AgNNPs films confirmed they have good UV barrier properties. SA-AgNNPs films exhibited excellent high antibacterial activity against foodborne pathogens including L. monocytogenes, S. enterica, and E. coli at the concentration of 500 µg/mL. Moreover, during the storage of bananas, SA-AgNNPs nanocomposite coatings act as a barrier to microbial contamination and slow down the ripening of bananas. As a result, compared with SA-coated and uncoated bananas, SA-AgNNPs-coated bananas exhibited the lowest weight loss and lowest total bacterial colonies, thus greatly extending their shelf life. Particularly when coated with SA-AgNNPs films, total bacterial colonies (TBC) in the banana peel and pulp were as low as 1.13 × 103 and 51 CUF/g on the ninth day of storage, respectively. Our work offers an efficient strategy to improve the quality of bananas during the postharvest period, with extensive applications in fruit preservation and food packing.
Natural Products and Health
Authors: Bartkowiak-Wieczorek Joanna , Mądry Edyta
Published: January 2024

A natural product is an organic compound from a living organism that can be isolated from natural sources or synthesized [...]
Utilization of Biopolymer-Based Lutein Emulsion as an Effective Delivery System to Improve Lutein Bioavailability in Neonatal Rats
Authors: Zhang Yanqi , Kong Lingyan , Lawrence Jeannine C. , Tan Libo
Published: January 2024

Newborns’ eyes and brains are prone to oxidative stress. Lutein has antioxidant properties and is the main component of macular pigment essential for protecting the retina, but has low bioavailability, thereby limiting its potential as a nutritional supplement. Oil-in-water emulsions have been used as lutein delivery systems. In particular, octenylsuccinated (OS) starch is a biopolymer-derived emulsifier safe to use in infant foods, while exhibiting superior emulsifying capacity. This study determined the effects of an OS starch-stabilized lutein emulsion on lutein bioavailability in Sprague-Dawley neonatal rats. In an acute study, 10-day-old pups received a single oral dose of free lutein or lutein emulsion, with subsequent blood sampling over 24 h to analyze pharmacokinetics. The lutein emulsion group had a 2.12- and 1.91-fold higher maximum serum lutein concentration and area under the curve, respectively, compared to the free lutein group. In two daily dosing studies, oral lutein was given from postnatal day 5 to 18. Blood and tissue lutein concentrations were measured. The results indicated that the daily intake of lutein emulsion led to a higher lutein concentration in circulation and key tissues compared to free lutein. The OS starch-stabilized emulsion could be an effective and safe lutein delivery system for newborns.
Effects of Egg Consumption on Subjects with SLD or Hypertension: A MICOL Study
Authors: Tatoli Rossella , Bonfiglio Caterina , Cuccaro Francesco , Campanella Angelo , Coletta Sergio , Pesole Pasqua Letizia , Giannelli Gianluigi , Donghia Rossella
Published: January 2024

Background: Steatotic liver disease (SLD) is defined as a fat accumulation in more than 5% of hepatocytes; it can progress to non-alcoholic steatohepatitis (NASH), associated with an increased state of inflammation. The aim of this study was to explore the protective effects of eating eggs and any association with SLD and hypertension (HTN). Methods: The study cohort included 908 participants assessed in the fourth recall of the MICOL study, grouped into four groups, based on NALFD and/or HTN. Results: The prevalence of HTN and SLD among participants was 31.61%. Overall, the results indicated a statistical significance of egg consumption, showing a protective role against the two disease conditions, in both the raw and adjusted models (RRR = 0.34, p = 0.009, 0.15 to 0.76 95% C.I.). Conclusions: Many differences were found among the groups, and the protective role of eating eggs was amply demonstrated. We can conclude that it is unwise to demonize the intake of this food and its nutritional properties, in contrast with previous reports in the literature.
First Insights on the Bioaccessibility and Absorption of Anthocyanins from Edible Flowers: Wild Pansy, Cosmos, and Cornflower
Authors: Teixeira Margarida , De Luca Lorenzo , Faria Ana , Bordiga Matteo , de Freitas Victor , Mateus Nuno , Oliveira Hélder
Published: January 2024

Edible flowers are regaining interest among both the scientific community and the general population, not only for their appealing sensorial characteristics but also from the growing evidence about their health benefits. Among edible flowers, those that contain anthocyanins are among the most consumed worldwide. However, little is known regarding the bioaccessibility and absorption of their bioactive compounds upon ingestion. The aim of this work was to explore, for the first time, the behavior of anthocyanin-rich extracts from selected edible flowers under different food processing conditions and after ingestion using simulated digestions, as well as their absorption at the intestinal level. Overall, the results showed that the monoglucoside and rutinoside anthocyanin extracts were less stable under different pH, temperature, and time conditions as well as different digestive processes in the gastrointestinal tract. There was a prominent decrease in the free anthocyanin content after the intestinal phase, which was more pronounced for the rutinoside anthocyanin extract (78.41% decrease from the oral phase). In contrast, diglucoside and rutinoside anthocyanin extracts showed the highest absorption efficiencies at the intestinal level, of approximately 5% after 4 h of experiment. Altogether, the current results emphasize the influence of anthocyanins’ structural arrangement on both their chemical stability as well as their intestinal absorption. These results bring the first insights about the bioaccessibility and absorption of anthocyanins from wild pansy, cosmos, and cornflower and the potential outcomes of such alternative food sources.
Combating Acute Myeloid Leukemia via Sphingosine Kinase 1 Inhibitor-Nanomedicine Combination Therapy with Cytarabine or Venetoclax
Authors: Nguyen Thao M. , Joyce Paul , Ross David M. , Bremmell Kristen , Jambhrunkar Manasi , Wong Sook S. , Prestidge Clive A.
Published: January 2024

MP-A08 is a novel sphingosine kinase 1 (SPHK1) inhibitor with activity against acute myeloid leukemia (AML). A rationally designed liposome-based encapsulation and delivery system has been shown to overcome the physicochemical challenges of MP-A08 and enable its effective delivery for improved efficacy and survival of mice engrafted with human AML in preclinical models. To establish therapies that overcome AML’s heterogeneous nature, here we explored the combination of MP-A08-loaded liposomes with both the standard chemotherapy, cytarabine, and the targeted therapy, venetoclax, against human AML cell lines. Cytarabine (over the dose range of 0.1–0.5 µM) in combination with MP-A08 liposomes showed significant synergistic effects (as confirmed by the Chou–Talalay Combination Index) against the chemosensitised human AML cell lines MV4-11 and OCI-AML3. Venetoclax (over the dose range of 0.5–250 nM) in combination with MP-A08 liposomes showed significant synergistic effects against the chemosensitised human AML cell lines, particularly in venetoclax-resistant human AML cells. This strong synergistic effect is due to multiple mechanisms of action, i.e., inhibiting MCL-1 through SPHK1 inhibition, leading to ceramide accumulation, activation of protein kinase R, ATF4 upregulation, and NOXA activation, ultimately resulting in MCL-1 degradation. These combination therapies warrant further consideration and investigation in the search for a more comprehensive treatment strategy for AML.
Onchocerciasis Drug Discovery: In Vitro Evaluation of FDA-Approved Drugs against Onchocerca gutturosa in Gambia
Authors: Gokool Suzanne , Townson Simon , Freeman Andrew , Siemienski-Kleyn Jadzia , Zubrzycki Jakub , Tagboto Senyo , Hübner Marc P. , Scandale Ivan
Published: January 2024

Onchocerciasis treatment and control relies mainly on the use of ivermectin which has high activity against the microfilarial stage of Onchocerca volvulus but limited activity against the long-lived, tissue dwelling adult nematodes. As this neglected tropical disease has now been targeted for elimination, there is an urgent need for new drugs to combat these parasites, ideally with macrofilaricidal activity. In this study, we have examined the anti-Onchocerca activity of a range of existing FDA-approved drugs with a view to repurposing, which can lead to rapid and relatively inexpensive development. From the Pharmakon-1600 library, 106 drugs were selected and tested against O. gutturosa adult male parasites using a concentration of 1.25 × 10−5 M in an in vitro 5-day standard assay to assess motility and viability (using MTT/formazan colorimetry). The findings revealed that 44 drugs produced marginal/moderate activity (50–99% motility and/or MTT reductions) including cefuroxime sodium, methenamine, primaquine phosphate and rivastigmine tartrate, while 23 drugs produced good activity (100% motility reductions and significant MTT reductions), including atovaquone, isradipine, losartan, rifaximin, cefaclor and pyrantel pamoate. Although this study represents only a first step, some of the identified hits indicate there are potential anti-Onchocerca drug candidates worthy of further investigation.
Improved Modeling of Temperature Evolution during Lung Cancer Tumor Thermal Ablation
Authors: Selmi Marwa
Published: January 2024

Microwave ablation (MWA) represents one of the most powerful tools in cancer treatment. This therapeutic modality process is governed by the temperature and absorbed dose of radiation of the cell tissue. This study was performed to control the temperature effect using simulation during the MWA thermal damage of lung tumor. For this reason, a two-dimensional (2D) computational modeling generated for adaptive lung tissue was designed and analyzed using the finite element method (FEM). Different approaches, such as first-order Arrhenius rate equations, Maxwell equations, and the bioheat equation, have been used to simulate necrosis in cells. To control the heat, a proportional–integral–derivative (PID) controller was used to moderate the input microwave power source and to maintain the temperature of the target tip at a lower level of the initial temperature data. Furthermore, full cancer tissue necrosis was also evaluated by processing time and thermal damage fraction. The obtained data proved that the target tip temperature was affected by the temperature distribution and specific absorption rate (SAR). However, a specific treatment period of tumor ablation is required to control and decrease the damage of surrounding healthy tissue to ensure a safe operation without any risk.