bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential of Hydrogen Fuel Cell Aircraft for Commercial Applications with Advanced Airframe and Propulsion Technologies
Authors: Karpuk Stanislav , Freund Yannik , Hanke-Rauschenbach Richard
Published: January 2025

The present work demonstrates a comparative study of hydrogen fuel cells and combustion aircraft to investigate the potential of fuel cells as a visionary propulsion system for radically more sustainable medium- to long-range commercial aircraft. The study, which considered future airframe and propulsion technologies under the Se2A project, was conducted to quantify potential emissions and costs associated with such aircraft and to determine the benefits and drawbacks of each energy system option for different market segments. Future technologies considered in the present work include laminar flow control, active load alleviation, new materials and structures, ultra-high bypass ratio turbofan engines, more efficient thermal management systems, and superconducting electric motors. A multi-fidelity initial sizing framework with coupled constraint and mission analysis blocks was used for parametric airplane sizing and calculations of all necessary characteristics. Analyses performed for three reference aircraft of different sizes and ranges concluded that fuel-cell aircraft could have operating cost increases in the order of 30% compared to hydrogen combustion configurations and were caused by substantial weight and fuel burn increases. In-flight changes in emissions of fuel cell configurations at high altitudes were progressively reduced from medium-range to long-range segments from being similar to hydrogen combustion for medium-range to 24% for large long-range aircraft, although fuel cell aircraft consume 22–30% more fuel than combustion aircraft. Results demonstrate a positive environmental impact of fuel cell propulsion for long-range applications, the possibilities of being a more emission-universal solution, if desired optimistic technology performance metrics are satisfied. The study also demonstrates progressively increasing technology requirements for larger aircraft, making the long-range application’s feasibility more challenging. Therefore, substantial development of fuel cell technologies for long-range aircraft is imperative. The article also emphasizes the importance of airframe and propulsion technologies and the necessity of green hydrogen production to achieve desired emissions.
A Novel Hydraulic Interconnection Design and Sliding Mode Synchronization Control of Leveling System for Crawler Work Machine
Authors: Wang Ruochen , Zhang Kaiqiang , Ding Renkai , Jiang Yu , Jiang Yiyong
Published: January 2025

To address the issues of easy overturning and poor safety of crawler work machines operating on steep slopes in hilly and mountainous areas, this study develops a structural design scheme based on a “three-layer frame” structure. An omnidirectional leveling system with hydraulic interconnection is designed to maintain platform stability by ensuring a stationary central point during leveling. Furthermore, a sliding mode synchronization control method based on a disturbance observer is proposed to reduce the synchronization error of the hydraulic cylinders and enhance leveling precision. The system’s performance is validated through an AMESim-MATLAB/Simulink co-simulation platform, demonstrating significant improvements over traditional PID control. Specifically, both lateral and longitudinal leveling times are reduced, rise time decreases by 21.8% on average, and overall leveling time is reduced by 35.5%, with synchronization errors maintained within ±6 × 10−4 m. Finally, physical prototype testing further confirms the system’s effectiveness, achieving an average body inclination error of 2.55% and a hydraulic cylinder synchronization error of 8.2%. These findings validate the feasibility and superiority of the proposed omnidirectional leveling system for the crawler work machine in hilly and mountainous regions.
Evaluating Copper-Doped Biochar Composites for Improving Wheat Nutrition and Growth in Oxisols
Authors: Chisté Loren , Silva Carlos Alberto , Rabêlo Flávio Henrique Silveira , Jindo Keiji , Melo Leônidas Carrijo Azevedo
Published: January 2025

Copper (Cu) is a critical micronutrient for wheat (Triticum aestivum L.), essential for growth and grain baking quality, yet its availability is limited because Cu is specifically adsorbed on colloids of highly weathered tropical soils like Oxisols. This study hypothesizes that Cu-doped biochar composites can outperform traditional Cu fertilizers in improving wheat growth and Cu use efficiency. Composites were synthesized from chicken manure (FCM), shrimp shells (FSC), and sewage sludge (FSS), doped with copper sulfate (CuSO45H2O) or copper oxide (CuO), and pyrolyzed at 300 °C or 550 °C. The experimental design involved greenhouse trials in two Oxisols (RYL and DRL), assessing Cu release kinetics, plant Cu uptake, and dry matter production. Fourier Transform Infrared Spectroscopy (FTIR) confirmed successful Cu integration. Results revealed that CSS/CS-5 (FSS + CuSO45H2O at 550 °C) improved Cu uptake and shoot biomass in DRL soil, while CSC/CS-3 (FSC + CuSO45H2O at 300 °C) enhanced wheat CuSO45H2O growth in RYL soil. Peak Cu availability varied by CuSO45H2O soil and composite type, with residual Cu highest CuSO45H2O in CuSO45H2O-treated soils. These findings demonstrate that Cu–biochar composites, tailored to soil conditions, offer a sustainable alternative to mineral Cu fertilizers by enhancing the nutrient availability and wheat grain yield.
Effects of Climate Variation on Spring Potato Growth, Yield, and Quality in South Korea
Authors: Park Hyun Hwa , Ei Ei , Kuk Yong In
Published: January 2025

In South Korea, spring potatoes account for over 60% of total potato production, but global warming and anomalous weather events may impact their growth and yield. This study examined potato cultivation practices across 12 locations with varying climates, analyzing meteorological factors, soil properties, and potato composition to identify stable cultivation areas. A survey of 45 farms revealed earlier planting dates in G3 regions compared to G2 and G1. Regions were classified into three groups (G1, G2, and G3) based on climatic conditions, with G1 representing the most temperate regions, G2 indicating regions with moderate climates, and G3 including areas with the warmest climates. The Superior variety was predominately cultivated in average areas of 1.4 ha. Yields ranged from 22,500 to 35,000 kg/ha, with G2 regions producing the highest yields. During tuber formation, plant height in G2 and G3 was greater than in G1, but no differences were noted at harvest. Planting times correlated with higher February and March temperatures, which were highest in G3. Soil properties were suitable across all regions, with minor variations. Ash and crude fat content were highest in G1 crops, while ascorbate and glutathione levels were highest in G3. No significant differences were found in total phenol and flavonoid content, though G2 and G3 showed higher antioxidant activity. Similar weather during the main growth period (April–June) minimized regional differences in growth, yield, and quality, but ongoing monitoring is recommended as climate change progresses. Overall, this study provides insights into how climatic conditions affect potato cultivation in South Korea and emphasizes the importance of adapting farming practices to ensure stable yields under changing climate conditions.
A Study on Zone-Based Secure Multicast Protocol Technique to Improve Security Performance and Stability in Mobile Ad-Hoc Network
Authors: Yang Hwanseok
Published: January 2025

Since MANET consists of only nodes and is in wireless communication with limited resources, cooperation between nodes is very important. Multicasting technology supports various applications that allow data to be immediately transmitted to target nodes. In environments such as MANET, where nodes are constantly moving, finding an efficient path from the source to the destination is a critical challenge. Providing integrity for the data transmitted from the source to the target node set is also an important part. Maintaining the state of neighbor nodes not only increases the communication and processing overhead but also requires more memory. In this paper, we propose a zone-based secure routing algorithm to improve the performance of routing protocols. We will also prove efficient management of node groups in an area and better scalability, performance, and security despite frequent topology changes by using group keys. To evaluate the proposed technique, detailed and extensive simulation performance with PAST-DM and MSZRP are evaluated. The simulation results show that the proposed technique, compared to other routing protocols, can achieve scalability by maintaining the routing load even if the speed of nodes, the number of sources, the number of group members, and the size of the network increase.
A Novel Ensemble Meta-Model for Enhanced Retinal Blood Vessel Segmentation Using Deep Learning Architectures
Authors: Chetoui Mohamed , Akhloufi Moulay A.
Published: January 2025

Background: Retinal blood vessel segmentation plays an important role in diagnosing retinal diseases such as diabetic retinopathy, glaucoma, and hypertensive retinopathy. Accurate segmentation of blood vessels in retinal images presents a challenging task due to noise, low contrast, and the complex morphology of blood vessel structures. Methods: In this study, we propose a novel ensemble learning framework combining four deep learning architectures: U-Net, ResNet50, U-Net with a ResNet50 backbone, and U-Net with a transformer block. Each architecture is customized to enhance feature extraction and segmentation performance. The models are trained on the DRIVE and STARE datasets to improve the degree of generalization and evaluated using the performance metric accuracy, F1-Score, sensitivity, specificity, and AUC. Results: The ensemble meta-model integrates predictions from these architectures using a stacking approach, achieving state-of-the-art performance with an accuracy of 0.9778, an AUC of 0.9912, and an F1-Score of 0.8231. These results demonstrate the performance of the proposed technique in identifying thin retinal blood vessels. Conclusions: A comparative analysis using qualitative and quantitative results with individual models highlights the robustness of the ensemble framework, especially under conditions of noise and poor visibility.
TLR7: A Key Prognostic Biomarker and Immunotherapeutic Target in Lung Adenocarcinoma
Authors: Hu Feiming , Hu Chenchen , He Yuanli , Sun Yuanjie , Han Chenying , Zhang Xiyang , Yu Lingying , Shi Daimei , Sun Yubo , Zhang Junqi , Jiang Dongbo , Yang Shuya , Yang Kun
Published: January 2025

Background: The tumor microenvironment (TME) plays a crucial role in the progression of lung adenocarcinoma (LUAD). However, understanding its dynamic immune and stromal modulation remains a complex challenge. Methods: We utilized the ESTIMATE algorithm to evaluate the immune and stromal components of the LUAD TME from the TCGA database. Correlations between these components and clinical characteristics and patient prognosis were analyzed. Toll-like receptor 7 (TLR7) was identified as a key prognostic biomarker through PPI network and COX regression analysis. Validation of TLR7 expression was conducted using GEO data, qPCR, WB, and IHC. A prognostic model was developed using a nomogram, incorporating TLR7 expression. Enrichment analysis, the Tumor Immune Estimation Resource database, and single-sample gene set enrichment analysis were used to explore TLR7’s potential function. The response of the TLR7 subgroup to immunotherapy and drug sensitivity was observed. Results: We found significant associations between the immune and stromal components of LUAD TME and clinical features and prognosis. Specifically, TLR7 was identified as a prognostic biomarker, where lower expression in tumor tissues was linked to worse outcomes. This finding was further confirmed by comparing TLR7 expression in LUAD cells to normal bronchial epithelial cells, revealing lower expression in the tumor cells. Incorporating TLR7 into a nomogram prognostic model resulted in a good predictor of patient survival. Additionally, TLR7 was associated with immune function and positively correlated with various immune cells. Importantly, patients with high TLR7 expression were more likely to benefit from anti-PD-1 checkpoint blockade therapy. We also identified four treatment candidates for patients with high TLR7 expression. Conclusion: TLR7 is a powerful clinical feature that predicts patient prognosis, immunotherapeutic response, and drug candidates, providing additional insights for the treatment of LUAD.
Proteomic Characterization of Liver Cancer Cells Treated with Clinical Targeted Drugs for Hepatocellular Carcinoma
Authors: Long Hezhou , Zhou Jiafu , Zhou Changxia , Xie Shuyu , Wang Jingling , Tan Minjia , Xu Junyu
Published: January 2025

Background/Objectives: Hepatocellular carcinoma (HCC) remains a significant global health concern, primarily due to the limited efficacy of targeted therapies, which are often compromised by drug resistance and adverse side effects. Methods: In this study, we utilized a Tandem Mass Tag (TMT)-based quantitative proteomic approach to analyze global protein expression and serine/threonine/tyrosine (S/T/Y) phosphorylation modifications in HepG2 cells following treatment with three clinically relevant hepatocellular carcinoma-targeted agents: apatinib, regorafenib, and lenvatinib. Results: Utilizing KEGG pathway enrichment analysis, biological process enrichment analysis, and protein interaction network analysis, we elucidated the common and specific metabolic pathways, biological processes, and protein interaction regulatory networks influenced by three liver cancer therapeutics. The study additionally proposed potential combinational treatment strategies, highlighting a possible synergistic interaction between HCC-targeted drugs and the DNA methyltransferase inhibitor. Furthermore, through the integration of clinical phosphorylation site data, we identified several phosphorylation sites that exhibited higher abundance in tumor tissues compared to adjacent non-tumor tissues. These sites were associated with poor prognosis and elevated functional scores. Conclusions: In summary, this study conducted an in-depth analysis of the molecular alterations in proteins and phosphorylation modifications induced by clinical HCC-targeted drugs, predicting drug combination strategies and therapeutic targets.
Fluorescence Polarization Immunoassay for Rapid, Sensitive Detection of the Herbicide 2,4-Dichlorophenoxyacetic Acid in Juice and Water Samples
Authors: Mukhametova Liliya I. , Kolokolova Marya K. , Shevchenko Ivan A. , Tupertsev Boris S. , Zherdev Anatoly V. , Xu Chuanlai , Eremin Sergei A.
Published: January 2025

2,4-Dichlorophenoxyacetic acid (2,4-D) is one of the popular herbicides that is widely used in agriculture and can be found in food and water. A rapid and sensitive fluorescence polarization immunoassay (FPIA) was proposed for the detection of 2,4-D in juice and water. New tracers, 2,4-D-buthylenediamin fluoresceinthiocarbamyl (2,4-D-BDF) and 2,4-D-glycine aminofluorescein (2,4-D-GAF), were obtained and characterized. Monoclonal antibodies (MAb) obtained against 2,4-D were used as a recognition reagent. The kinetics of the interaction of MAb and tracers were studied, and the kinetic parameters of their binding were calculated. High specificity of binding of tracers and MAb was shown. In this work, an approach was elaborated on to reduce the detection limit of 2,4-D by the FPIA method by changing the volume of the studied sample. The optimized FPIA in a competitive format was characterized by the LODs of 2,4-D 8 and 0.4 ng/mL and the working ranges 30–3000 ng/mL and 3–300 ng/mL for juice and water, respectively. The entire test cycle (from sample receipt to evaluation of the analysis results) took only 20 min. The test for the recovery of 2,4-D in juice and water gave values from 95 to 120%, which demonstrated the reliability of the herbicide determination in real samples.
Carboxylated Graphene: An Innovative Approach to Enhanced IgA-SARS-CoV-2 Electrochemical Biosensing
Authors: de Souza Freire Luciana , Gandarilla Ariamna María Dip , Barcelay Yonny Romaguera , Ruzo Camila Macena , Salgado Barbara Batista , Tavares Ana P. M. , Nobre Francisco Xavier , de Souza Neto Julio Nino , Astolfi-Filho Spartaco , Țălu Ștefan , Lalwani Pritesh , Patra Niranjan , Brito Walter Ricardo
Published: January 2025

Biosensors harness biological materials as receptors linked to transducers, enabling the capture and transformation of primary biorecognition signals into measurable outputs. This study presents a novel carboxylation method for synthesizing carboxylated graphene (CG) under acidic conditions, enhancing biosensing capabilities. The characterization of the CG was performed using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), Raman spectroscopy, thermogravimetric analysis (TGA), and X-ray diffraction (XRD). We modified screen-printed carbon electrodes (SPCEs) with CG to immobilize the SARS-CoV-2 N-protein, facilitating targeted detection of IgA antibodies (IgA-SARS-CoV-2). The analytical performance was assessed via electrochemical techniques such as cyclic voltammetry and electrochemical impedance spectroscopy, confirming CG synthesis effectiveness and biosensor functionality. The developed biosensor efficiently detects IgA-SARS-CoV-2 across a dilution range of 1:1000 to 1:200 v/v in a phosphate-buffered saline (PBS) solution, with a limit of detection calculated at 1:1601 v/v. This device shows considerable potential because of its fast response time, miniaturized design facilitated by SPCEs, reduced sample volume requirements, high sensitivity and specificity, low detection limits, and signal enhancement achieved through nanomaterial integration.
Effect of Different Static Load Test Methods on the Performance of Combined Post-Grouted Piles: A Case Study in the Dongting Lake Area
Authors: Du Yu , Qi Kai , Zhang Run-Ze , Zhou Feng , Wan Zhi-Hui
Published: January 2025

To investigate the effect of combined end-and-shaft post-grouting on the vertical load-bearing performance of bridge-bored piles in the Dongting Lake area of Hunan, two post-grouted piles were subjected to bi-directional O-cell and top-down load tests before and after combined end-and-shaft grouting, based on the Wushi to Yiyang Expressway project. A comparative analysis was conducted on the bearing capacity, deformation characteristics, and load transfer behavior of the piles before and after grouting. This study also examined the conversion coefficient γ values of different soil layers obtained from the bi-directional O-cell test for bearing capacity calculations. Additionally, the characteristic values of the end bearing capacity, obtained from the bi-directional O-cell and top-down load tests, were compared with the values calculated using the relevant formulas in the current standards, which validated the accuracy of existing regulations and traditional loading methods. The results indicate that the stress distribution along the pile shaft differed between the two test methods. In the bi-directional O-cell test, the side resistance developed from the end to the head, while in the top-down load test, it developed from the head to the end. After combined post-grouting, the ultimate bearing capacity of the piles significantly increased, with side resistance increasing by up to 81.03% and end resistance by up to 105.66%. The conversion coefficients for the side resistance in silty sand and gravel before and after grouting are 0.86 and 0.80 and 0.81 and 0.69, respectively. The characteristic values of the end bearing capacity, as measured by the bi-directional O-cell and top-down load tests, were substantially higher than those calculated using the current highway bridge and culvert standards, showing increases of 133.63% and 86.15%, respectively. These findings suggest that the current standard formulas are overly conservative. Additionally, the measured values from the top-down load test may underestimate the actual bearing capacity of piles in engineering projects. Therefore, it is recommended that future pile foundation designs incorporate both bi-directional O-cell testing and combined post-grouting techniques to optimize design solutions.
Dual Biologic or Small Molecule Therapy in Pediatric Inflammatory Bowel Disease: A Single Center Experience
Authors: Guo Cheng , Zhou Jin , Wang Guoli , Wu Jie
Published: January 2025

Purpose: Currently, there is no clinical data reported on the therapy of dual biological agents in pediatric-onset inflammatory bowel disease (PIBD) patients in China. The purpose of this study was to evaluate the efficacy and safety of dual biologic therapy or biologics combined with small molecule drugs in refractory PIBD patients in China. Methods: Clinical, laboratory, endoscopic, and ultrasound data of PIBD patients from the Department of Gastroenterology of Beijing Children’s Hospital between January 2021 and October 2024 were retrospectively analyzed. PIBD patients who received dual biologic treatment or a combination of biologic and small molecule therapy were included in this study. Steroid-free clinical remission and adverse events were recorded. Results: In this retrospective study, out of 520 children with IBD, twelve children (2.3%) were diagnosed with refractory PIBD and met the criteria for dual biotherapy, including four with UC (33%) and eight with CD (67%). The median age of patients was 13.64 (range, 1.2–17.1) years at eligibility for dual biologic therapy. There are eight (67%) patients treated with infliximab/ustekinumab (IFX + UST), three (25%) patients with upadacitinib/ustekinumab (UPA + UST), one (8%) patient with infliximab/vedolizumab (IFX + VDZ). At 3, 6, and 12 months of dual biological treatment, 91.2% (11/12), 100% (12/12), and 100% (12/12) patients showed steroid-free clinical remission, respectively. The median fecal calprotectin decreased significantly from 1852.5 µg/g (IQR, 762.5–1988.25) at baseline to 359.0 (IQR, 217.5–730.25) μg/g at 3 months, 113 (IQR, 73.7–256) μg/g at 6 months, and 82.5 (IQR, 40.25–122.25) μg/g at 12 months. Only one CD patient with IFX + UST reported mild elevation of aminotransferase, who recovered after symptomatic treatment. Conclusions: Dual biologic or small molecule therapy may be effective and safe for children with refractory PIBD in China.
Canal of Nuck Cyst vs. Inguinal Hernia: A Case Report and Further Considerations
Authors: Prevezanos Dionysios , Doudakmanis Christos , Theocharis Stamatios , Kykalos Stylianos , Nikiteas Nikolaos I. , Tsourouflis Gerasimos
Published: January 2025

Background: Abnormalities in the canal of the Nuck are rare clinical entities with presentation in the early adulthood of females. Given their rarity, they can be misdiagnosed with a strangulated inguinal hernia. Methods: Herein, we report a young female with painful swelling of the right inguinal hernia and its surgical approach. Results: A 37-year-old female presented to the emergency department with pain in the right lower abdominal quadrant and an edematous inguinal mass. Physical examination showed a palpable inguinal mass, setting the suspicion for possible incarcerated inguinal hernia. Laboratory exams were within normal ranges. The patient was subsequently treated surgically. During the operation, a cystic well-rounded mass was identified and completely excised. The mesh and plug technique was the chosen technique for the restoration of the inguinal canal. The hospitalization length was one day, and the post-operative course was uneventful. Conclusions: Although a Nuck cyst is a rare entity, it should be taken into consideration in female adults who present with an inguinal mass.
Adaptive Hosting Capacity Forecasting in Distribution Networks with Distributed Energy Resources
Authors: Islam Md Tariqul , Hossain M. Jahangir , Habib Md. Ahasan , Zamee Muhammad Ahsan
Published: January 2025

The sustainable integration of distributed energy resources (DER) into distribution networks requires accurate forecasting of hosting capacity. The network and DER variables alone do not capture the full range of external influences on DER integration. Traditional models often overlook the dynamic impacts of these exogenous factors, leading to suboptimal predictions. This study introduces a Sensitivity-Enhanced Recurrent Neural Network (SERNN) model, featuring a sensitivity gate within the neural network’s memory cell architecture to enhance responsiveness to time-varying variables. The sensitivity gate dynamically adjusts the model’s response based on external conditions, allowing for improved capture of input variability and temporal characteristics of the distribution network and DER. Additionally, a feedback mechanism within the model provides inputs from previous cell states into the forget gate, allowing for refined control over input selection and enhancing forecasting precision. Through case studies, the model demonstrates superior accuracy in hosting capacity predictions compared to baseline models like LSTM, ConvLSTM, Bidirectional LSTM, Stacked LSTM, and GRU. Study shows that the SERNN achieves a mean absolute error (MAE) of 0.2030, a root mean square error (RMSE) of 0.3884 and an R-squared value of 0.9854, outperforming the best baseline model by 48 per cent in MAE and 71 per cent in RMSE. Additionally, Feature engineering enhances the model’s performance, improving the R-squared value from 0.9145 to 0.9854. The sensitivity gate also impacts the model’s performance, lowering MAE to 0.2030 compared to 0.2283 without the sensitivity gate, and increasing the R-squared value from 0.9152 to 0.9854. Incorporating exogenous factors such as the time of day as a sensitivity gate input, further improves responsiveness, making the model more adaptable to real-world conditions. This advanced SERNN model offers a reliable framework for distribution network operators, supporting intelligent planning and proactive DER management. Ultimately, it provides a significant step forward in hosting capacity analysis, enabling more efficient and sustainable DER integration within next-generation distribution networks.
A Comprehensive Review of the Thermophysical Properties of Energetic Ionic Liquids
Authors: Bablee Aishorjo , Amarasekara Ananda , Gabitto Jorge , Bhuiyan Ariful , Shamim Nabila
Published: January 2025

Energetic ionic liquids (EILs) have various industrial applications because they release chemically stored energy under certain conditions. They can avoid some environmental problems caused by traditionally used toxic fuels. EILs, which are environmentally friendly and safer, are emerging as an alternative source for hypergolic bipropellant fuels. This review focuses on the crucial thermophysical properties of the EILs. The properties of imidazolium and triazolium-based ionic liquids (ILs) are discussed here. The thermophysical properties addressed, such as glass transition temperature, viscosity, and thermal stability, are critical for designing EILs to meet the need for sustainable energy solutions. Imidazolium-based ILs have tunable physical properties making them ideal for use in energy storage while triazolium-based ILs have thermal stability and energetic potential. As a result, it is important to understand and compile thermophysical properties so they can help researchers synthesize tailored compounds with desirable characteristics, advancing their application in energy storage and propulsion technologies.
The Effect of Backfill Gas Pressure on the Thermal Response of a Dry Cask for Spent Nuclear Fuel
Authors: Angelucci Michela , Cancemi Salvatore A. , Lo Frano Rosa , Paci Sandro
Published: January 2025

Dry systems are being employed worldwide as interim storage for Spent Nuclear Fuel (SNF). Despite not being designed as permanent repositories, the safe storage of SNF must still be ensured. In this framework, few experimental campaigns have been conducted in the past. However, their limited number has led to the necessity to exploit numerical simulations for the thermal characterization of the system. Since the majority of the degradation mechanisms are temperature-dependent, conducting a thermal analysis of a dry cask is essential to assess the integrity of the system itself, and of the SNF stored within it. In this regard, both heat production and heat removal mechanisms have to be taken into account. On this basis, the present paper addresses the variation in the system heat removal capacity when considering different backfill gas pressures. In particular, the analysis, carried out with the MELCOR code, investigates the thermal response of the ventilated, concrete-based HI-STORM 100S cask, currently employed for spent fuel elements of Light Water Reactors (LWRs), when imposing different initial pressures for the helium backfill gas. Results are reported primarily in terms of maximum temperature of the fuel cladding, which is the variable under regulatory surveillance. In addition, the adherence to the maximum design pressure for the canister is verified by evaluating the helium pressure as the steady state is reached. The analysis seems to suggest that the safe operation of the HI-STORM 100S cask is guaranteed only for a limited range of the initial helium pressure.
Dynamic Trends in Aquatic Product Supply and Consumption in China: Implications for Sustainable Diets and Environmental Impact Reduction
Authors: Yang Wanni
Published: January 2025

Aquatic foods play a pivotal role in transforming food systems. As the world’s leading producer, consumer, and trader of aquatic products, China’s potential for sustainable supply and consumption is critical to understand. The aim of this study was to depict the dynamic trends of aquatic products and the consequences of sustainable diets and environmental impacts. A panel dataset about Chinese aquatic products covering the period from 1952 to 2023 was drawn for analysis. Diet sustainability was assessed with the deviation from the Dietary Guidelines for Chinese Residents (2022) and EAT-Lancet recommendations. The environmental impacts of aquatic products’ supply and consumption were assessed using carbon footprints and water footprints. The findings reveal that aquatic products’ supply increased from 4.65 million tons to 71.16 million tons from 1978 to 2023, and annual aquatic food consumption per capita increased from 3.50 kg in 1978 to 15.20 kg. While overall supply meets consumption needs, structural imbalances persist at the provincial level. Over time, the influence of marine fishery products has declined from 1.06 million tons (63.63%) in 1952 to 35.85 million tons (50.38%) in 2023, whereas offshore aquaculture shows promising potential for meeting future supply demands (23.96 million tons in 2023, accounting for 66.82% of marine fishery production). To align with healthy dietary goals and environmentally sustainable food systems, provincial aquatic food demand across China was adjusted. The carbon footprints and water footprints of both current and adjusted consumption patterns were also assessed. The results indicate that adjusting consumption based on the Dietary Guidelines for Chinese Residents (2022) and EAT-Lancet recommendations could reduce environmental impacts to different degrees. The findings could offer valuable references and insights into developing sustainable strategies in aquatic product management and advancing food system transformation.
Studies on Quality Deterioration and Metabolomic Changes in Oysters Induced by Spoilage Bacteria During Chilled Storage
Authors: Dou Hanzheng , Zhu Wenxiu , Chen Siyang , Zou Yue , Xia Xiaodong
Published: January 2025

The correlation between spoilage bacteria and the degradation of aquatic food quality during chilled storage is substantial. However, our understanding of the precise roles of spoilage bacteria in oyster spoilage remains incomplete. The aim of this study was to explore the role of three dominant spoilage bacteria strains in oyster spoilage. Subsequently, the metabolites of spoiled oyster meat after inoculation with bacteria were analyzed using LC-MS-based untargeted metabolomics. Combining the results from various biochemical indicators of spoilage, Psychrobacter immobilis, Shewanella putrefaciens, and Photobacterium swingsii are shown to be the main spoilage bacteria in spoiled oyster meat, and their effects on changes in oyster meat quality were evaluated through total volatile basic nitrogen (TVB-N), pH, thiobarbituric acid reactive substances (TBARSs), and weight loss, respectively. The results showed that Ps. immobilis and S. putrefaciens exhibited great spoilage capacity. P swingsii, although a dominant spoilage bacterium, exhibited lower spoilage competency than the above two bacterial strains but demonstrated activity in producing microbial lipases to oxidize fats. In addition, the results of the metabolomics of spoiled oyster meat suggest that 7, 8-Dimethoxy-3-(4-methoxyphenyl)-4-oxo-4H-chromen-5-yl-2-O-pentopyranosylhexopyranoside, 1,2,3,6-Tetrahydropyridine-4-carboxylic acid, Propionic acid, and L-phenylalanine are potential markers of spoilage in oysters. These findings extend our understanding of the roles that microorganisms play in the spoilage of oysters and offer valuable insights into the development of technologies for monitoring the freshness of oysters based on these potential spoilage markers.
Hydrogel-Based Innovations in Carpal Tunnel Syndrome: Bridging Pathophysiological Complexities and Translational Therapeutic Gaps
Authors: Dinescu Venera Cristina , Martin Liviu , Bica Marius , Vasile Ramona Constantina , Gresita Andrei , Bunescu Marius , Ruscu Mihai Andrei , Aldea Madalina , Rotaru-Zavaleanu Alexandra Daniela
Published: January 2025

Carpal Tunnel Syndrome (CTS) is a prevalent neuropathic disorder caused by chronic compression of the median nerve, leading to sensory and motor impairments. Conventional treatments, such as corticosteroid injections, wrist splinting, and surgical decompression, often fail to provide adequate outcomes for chronic or recurrent cases, emphasizing the need for innovative therapies. Hydrogels, highly biocompatible three-dimensional biomaterials with customizable properties, hold significant potential for CTS management. Their ability to mimic the extracellular matrix facilitates localized drug delivery, anti-adhesion barrier formation, and tissue regeneration. Advances in hydrogel engineering have introduced stimuli-responsive systems tailored to the biomechanical environment of the carpal tunnel, enabling sustained therapeutic release and improved tissue integration. Despite these promising developments, hydrogel applications for CTS remain underexplored. Key challenges include the absence of CTS-specific preclinical models and the need for rigorous clinical validation. Addressing these gaps could unlock the full potential of hydrogel-based interventions, which offer minimally invasive, customizable solutions that could improve long-term outcomes and reduce recurrence rates. This review highlights hydrogels as a transformative approach to CTS therapy, advocating for continued research to address translational barriers. These innovations have the potential to redefine the treatment landscape, significantly enhancing patient care and quality of life.
Expression of ABCB1, ABCC1, and LRP in Mesenchymal Stem Cells from Human Amniotic Fluid and Bone Marrow in Culture—Effects of In Vitro Osteogenic and Adipogenic Differentiation
Authors: Romão Carolina Martinez , de Lara Janz Felipe , Ruiz Jorge Luis Maria , Lopes Marco Antônio Borges , Cristante Alexandre Fogaça , de Barros Filho Tarcísio Eloy Pessoa , Levy Débora , Bydlowski Sérgio Paulo
Published: January 2025

Mesenchymal stem cells (MSCs) are multipotent cells with the potential to differentiate into various lineages. They have also the potential to protect themselves against harmful stimuli to maintain their functional integrity. Drug resistance-related transporters such as ABCB1 (P-glycoprotein; P-gp), ABCC1 (MRP1; multidrug resistance-related Protein 1), and LRP (lung resistance protein) may protect MSCs against toxic substances such as chemotherapeutic agents. This study evaluated ABCB1, ABCC1, and LRP before and after the differentiation of MSCs derived from human amniotic fluid (AF) and bone marrow (BM). P-gp expression in both AFMSCs and BMMSCs was analyzed by immunocytochemistry, and pump function was analyzed by cell viability assay with doxorubicin (DOX) and Rhodamine 123 (Rh 123) dye exclusion. ABCB1, ABCC1, and LRP gene expression was determined by RT-PCR both before and after osteogenic and adipogenic differentiation. The MES-SA/DX5 cell line was used as a model of resistance to DOX and the overexpression of P-gp. Both AFMSCs and BMMSCs displayed a high P-gp expression, although lower than MES-SA/DX5 control cells. It was shown that both, undifferentiated AFMSCs and BMMSCs, have high cell viability in response to DOX, similar to the MES-SA/DX5 lineage. ABCB1 was less expressed in BM than in AFMSCs in undifferentiated samples, while no differences were observed in the expression of ABCC1 and LRP. AFMSCs showed an increase in ABCB1 after osteogenic differentiation, whereas BMMSCs exhibited lower ABCB1 and ABCC1 expression after osteogenic and adipogenic differentiation. The findings suggest that ABCB1, ABCC1, and LRP gene expression in AFMSCs and BMMSCs is influenced by differentiation processes and further support the concept that these transporters modulate MSC differentiation in a cell source-dependent way.
Proteomic Profiling of Extracellular Vesicles in Inflammatory Bowel Diseases
Authors: Baldán-Martín Montse , Azkargorta Mikel , Lapitz Ainhoa , Ortega Moreno Lorena , Iloro Ibon , Fernández-Tomé Samuel , Arbelaiz Ander , Escobes Iraide , Marín Alicia C. , Bernardo David , Bujanda Luis , Bañales Jesús M. , Elortza Felix , Gisbert Javier P. , Chaparro María
Published: January 2025

The proteomic analysis of serum extracellular vesicles (EVs) could be a useful tool for studying the pathophysiology of Crohn’s disease (CD) and ulcerative colitis (UC), as well as for biomarker discovery. To characterize the proteomic composition of serum EVs in patients with CD and UC to identify biomarkers and molecular pathways associated with pathogenesis and activity. Methods: Serum EVs were enriched and analyzed in patients with quiescent CD, active CD (aCD), quiescent UC, active UC (aUC), and healthy controls (HCs) (n = 30 per group). All groups were matched for age and sex. Disease activity was assessed by ileocolonoscopy and categorized based on the SES-CD (CD) and the endoscopic sub-score of the Mayo Score (UC). EVs were enriched by ul-tracentrifugation, and their size and concentration were determined by nanoparticle tracking analysis. The expression of CD63, CD81, and CD9 was determined using West-ern blotting. Proteomic analysis was performed by label-free nano-LC MS/MS. A total of 324 proteins were identified; 60 showed differential abundance in CD-HC, 34 in UC-HC, and 21 in CD-UC. Regarding disease activity, the abundance of 58 and 32 proteins was altered in aCD-HC and aUC-HC, respectively. Functional analyses revealed that proteins associated with aCD were involved in immune regulation, whereas those linked to aUC were enriched in oxidative stress. We have identified expressed proteins between EVs from patients with CD and UC, depending on the presence of disease, the disease type, and the disease activity. These proteins are potential candidates as disease biomarkers and open new research avenues to better understand these conditions.
Genome De Novo (WGS) Sequence Resource of the Lasiodiplodia theobromae Bot-2018-LT45 Isolate Causing Dieback in Apple
Authors: Valdez-Tenezaca Adrián V. , Covarrubias Sergio A. Hernández , Murillo Carrasco Alexis G. , Peñalosa Matías I. Guerra , Figueroa Jean F. Castro , Delgado Fernández M. Ernesto , Corona-Gómez José A. , A. Díaz Ulloa Gonzalo
Published: January 2025

Lasiodiplodia theobromae is a pathogenic fungus associated with tropical perennial fruit plants worldwide. In apple trees, L. theobromae causes dieback and canker, a disease that affects the architecture of the wood producing the progressive death of branches and stems, from the tips to the base, invading the vascular tissue, manifesting necrotic lesions in the bark, impeding the flow of nutrients and water. The present work reports the whole genome de novo sequencing (WGS) of L. theobromae strain Bot-2018-LT45 isolated from apple trees with dieback symptoms. Genomic DNA of L. theobromae was sequenced using Illumina paired-end short-read technology (NovaSeq6000) and PacBio SMRTbellTM (Single Molecule, Real-Time) long-read technology. The genome size was 44.17 Mb. Then, assembly and annotation revealed a total of 12,948 genes of which 11,634 encoded proteins. The genome was assembled into 34 contigs with an N50 (Mb) value of 3.23. This study is the first report of the L. theobromae genome de novo obtained from apple trees with dieback and canker symptoms in the Maule Region, Chile. This genetic information may set the basis for future study of the mechanisms of L. theobromae and establish the possibility of specific molecular improvements for the control of dieback and canker.
Synthesis and Characterization of Pd/La2O3/ZnO Catalyst for Complete Oxidation of Methane, Propane and Butane
Authors: Velinova Ralitsa , Kaneva Nina , Ivanov Georgi , Kovacheva Daniela , Spassova Ivanka , Todorova Silviya , Atanasova Genoveva , Naydenov Anton
Published: January 2025

The catalytic oxidation of volatile organic compounds (VOCs) is the subject of considerable interest due to its applications in environmental protection. Noble metal-based catalysts are widely employed to remove toxic compounds from gas mixtures. The objective of the present study was the synthesis of a palladium-containing catalyst deposited on a support modified with La2O3 zinc oxide. The composite support was initially obtained by a simple method, and then palladium was deposited on it by impregnation. Various methods, including N2-physisorption, XRD, HRTEM, XPS, TPD, TPR, and FTIR, were used to characterize the material. The obtained catalyst was studied in the reaction of the complete oxidation of butane, propane, and methane. It was found that the addition of La2O3 to ZnO led to an improved pore texture. The catalytic tests showed that the reaction of the complete oxidation of butane on Pd/La2O3/ZnO proceeded at the lowest temperatures.
Localization of Microvascular Changes in Systemic Disease Without Retinopathy Using Optical Coherence Tomography Angiography (OCTA)
Authors: Hattenhauer Alex , Cabrera Kimberly , Locatelli Elyana V. T. , Donthineni Pragnya Rao , Goldhardt Raquel , Wang Jianhua , Galor Anat
Published: January 2025

Background: This study aimed to evaluate the location of retinal fractal dimension (FD) abnormalities in individuals with diabetes mellitus (DM) and hypertension (HTN) without retinopathy. Methods: The annular zone of 6 mm × 6 mm OCTA images centered on the fovea was partitioned into thin annuli and analyzed using fractal analysis to measure FDs. Results: The cohort (n = 114) had an average age of 55.7 years, with 87% self-identifying as male; 29% (n = 33) had HTN, 8% (n = 9) had DM, and 11% (n = 12) had both. Individuals with DM showed significantly lower FDs in the C5 partition of the deep vascular plexus (DVP), located 1.77 mm to 2.14 mm from the foveal center compared to controls without DM (1.57 ± 0.003 vs. 1.58 ± 0.006; p = 0.014). Those with both DM and HTN exhibited significantly lower FDs in the entire superficial vascular plexus (SVP) annulus (1.84 ± 0.01 vs. 1.85 ± 0.007; p = 0.006), as well as the C3 (1.58 ± 0.02 vs. 1.60 ± 0.02; p = 0.008), C4 (1.57 ± 0.002 vs. 1.57 ± 0.01; p = 0.036), C5 (1.56 ± 0.01 vs. 1.57 ± 0.008; p < 0.001), and C6 (1.58 ± 0.01 vs. 1.59 ± 0.008; p < 0.001) partitions of the SVP (1.03 mm to 2.50 mm from the foveal center) and the C4 (1.57 ± 0.008 vs. 1.58 ± 0.008; p = 0.015) and C5 (1.57 ± 0.01 vs. 1.58 ± 0.006; p = 0.012) partitions of the DVP (1.40 mm to 1.77 mm from the foveal center) compared to the controls with neither DM nor HTN. Conclusions: While our study examined FDs in a predominantly male veteran population, our findings align with prior studies that reported lower FDs in DM and HTN cohorts. Our study further localizes these microvascular changes, with the most prominent differences occurring at C5 and C6 of the SVP, representing an area between 1.77 and 2.50 mm from the center of the fovea. These data lay the groundwork for developing screening protocols to identify individuals at risk of developing vasculopathies.
Computationally Efficient Light Field Video Compression Using 5-D Approximate DCT
Authors: Sritharan Braveenan , Edussooriya Chamira U. S. , Wijenayake Chamith , Cintra R. J. , Madanayake Arjuna
Published: January 2025

Five-dimensional (5-D) light field videos (LFVs) capture spatial, angular, and temporal variations in light rays emanating from scenes. This leads to a significantly large amount of data compared to conventional three-dimensional videos, which capture only spatial and temporal variations in light rays. In this paper, we propose an LFV compression technique using low-complexity 5-D approximate discrete cosine transform (ADCT). To further reduce the computational complexity, our algorithm exploits the partial separability of LFV representations. It applies two-dimensional (2-D) ADCT for sub-aperture images of LFV frames with intra-view and inter-view configurations. Furthermore, we apply one-dimensional ADCT to the temporal dimension. We evaluate the performance of the proposed LFV compression technique using several 5-D ADCT algorithms, and the exact 5-D discrete cosine transform (DCT). The experimental results obtained with LFVs confirm that the proposed LFV compression technique provides a more than 250 times reduction in the data size with near-lossless fidelity with a peak-signal-to-noise ratio greater than 40 dB and structural similarity index greater than 0.9. Furthermore, compared to the exact DCT, our algorithms requires approximately 10 times less computational complexity.
Evaluating the Potential of Sea Surface Height Observations and Depth Datum Calculation Using GNSS/IMU Buoys
Authors: Kuo Chung-Yen , Lan Wen-Hau , Lee Chi-Ming , Kao Huan-Chin
Published: January 2025

This study evaluates the potential of GNSS/IMU buoys for sea surface height observations and depth datum verification. GNSS/IMU buoys were deployed alongside 34 tide gauges around Taiwan for synchronous sea surface height measurements. The collected GNSS data were processed through relative positioning and loosely coupled GNSS/IMU integration methods. Analysis revealed that the average of the means of the differences was −2.5 cm across all stations, indicating that most tide gauge datums agreed well with the GNSS/IMU buoy measurements. Significant discrepancies were observed at only a few stations, likely due to local subsidence. Notably, the Shuitou station showed a mean difference of 63.4 cm, resulting from its remarkable deviation from tidal zero since 2019, suggesting a potential datum issue. The mean of the standard deviation (STD) of the differences across the stations was 3.8 cm, with the highest STD observed at the Shuitou station (9.4 cm). These findings demonstrate that GNSS/IMU buoys can effectively complement tide gauge measurements for observing sea surface heights and defining the depth datum, particularly in areas where local vertical land movements affect tide gauge data accuracy.