bluuebunny/crossref_metadata_2025 huggingface dataset:
HPLC/MS characterization of Syzygium aromaticum L. and evaluation of its effects on peritoneal adhesion: Investigating the role of inflammatory cytokines, oxidative factors, and fibrosis and angiogenesis biomarkers
Authors: Moradi Elham , Rakhshandeh Hassan , Rahimi Baradaran Vafa , Ghadiri Mobarakeh , Hasanpour Maedeh , Iranshahi Mehrdad , Askari Vahid Reza
Published: January 2023

The dried flower bud of Syzygium aromaticum L. (S. aromaticum) (Myrtaceae), cloves, have been used for their analgesic and anti‐inflammatory activities. Peritoneal adhesion (PA) is the most common complication of abdominal and pelvic surgeries, which causes significant adverse effects and severe economic burden. The present study aimed to evaluate the preventive effect of S. extract (SAE) on PA formation in a rat model. Male Wistar 8‐week‐old rats were randomly divided into sham, control (received vehicle), and treatment (0.25%, 0.5%, and 1% w/v of SAE) groups. The adhesion and related factors were examined using the Nair scoring system and immunological and biochemical kits for the levels of inflammatory cytokines [interleukin (IL)‐6 and tumor necrosis factor (TNF)‐α], growth factors [transforming growth factor (TGF)‐β1 and vascular endothelial growth factor (VEGF)], oxidative [nitric oxide (NO) and malondialdehyde (MDA)], and anti‐oxidative [glutathione (GSH)] factors. Our results figured out that the adhesion score and IL‐6, TNF‐α, TGF‐β1, VEGF, NO, and MDA levels were significantly increased, but the GSH level was decreased in the control group compared to the sham group (p < 0.001–0.05). On the other hand, the 0.25% SAE group had a lower adhesion score, and IL‐6, TNF‐α, TGF‐β1, VEGF, NO, and MDA levels were significantly decreased compared with the vehicle group, and the level of GSH was increased (p < 0.001–0.05). SAE could efficiently reduce adhesion score and regulate inflammatory cytokines, oxidative and anti‐oxidative factors, and biomarkers of fibrosis and angiogenesis. Therefore, clove extract can be considered a potential candidate for PA management.
Diagnostic quality model (DQM): an integrated framework for the assessment of diagnostic quality when using AI/ML
Authors: Lennerz Jochen K. , Salgado Roberto , Kim Grace E. , Sirintrapun Sahussapont Joseph , Thierauf Julia C. , Singh Ankit , Indave Iciar , Bard Adam , Weissinger Stephanie E. , Heher Yael K. , de Baca Monica E. , Cree Ian A. , Bennett Shannon , Carobene Anna , Ozben Tomris , Ritterhouse Lauren L.
Published: January 2023

Background

Laboratory medicine has reached the era where promises of artificial intelligence and machine learning (AI/ML) seem palpable. Currently, the primary responsibility for risk-benefit assessment in clinical practice resides with the medical director. Unfortunately, there is no tool or concept that enables diagnostic quality assessment for the various potential AI/ML applications. Specifically, we noted that an operational definition of laboratory diagnostic quality – for the specific purpose of assessing AI/ML improvements – is currently missing.

Methods

A session at the 3rd Strategic Conference of the European Federation of Laboratory Medicine in 2022 on “AI in the Laboratory of the Future” prompted an expert roundtable discussion. Here we present a conceptual diagnostic quality framework for the specific purpose of assessing AI/ML implementations.

Results

The presented framework is termed diagnostic quality model (DQM) and distinguishes AI/ML improvements at the test, procedure, laboratory, or healthcare ecosystem level. The operational definition illustrates the nested relationship among these levels. The model can help to define relevant objectives for implementation and how levels come together to form coherent diagnostics. The affected levels are referred to as scope and we provide a rubric to quantify AI/ML improvements while complying with existing, mandated regulatory standards. We present 4 relevant clinical scenarios including multi-modal diagnostics and compare the model to existing quality management systems.

Conclusions

A diagnostic quality model is essential to navigate the complexities of clinical AI/ML implementations. The presented diagnostic quality framework can help to specify and communicate the key implications of AI/ML solutions in laboratory diagnostics.
Asymmetric horseshoe-like assembly of peroxisomal yeast oxalyl-CoA synthetase
Authors: Bürgi Jérôme , Lill Pascal , Giannopoulou Evdokia-Anastasia , Jeffries Cy M. , Chojnowski Grzegorz , Raunser Stefan , Gatsogiannis Christos , Wilmanns Matthias
Published: January 2023

Oxalyl-CoA synthetase from Saccharomyces cerevisiae is one of the most abundant peroxisomal proteins in yeast and hence has become a model to study peroxisomal translocation. It contains a C-terminal Peroxisome Targeting Signal 1, which however is partly dispensable, suggesting additional receptor bindings sites. To unravel any additional features that may contribute to its capacity to be recognized as peroxisomal target, we determined its assembly and overall architecture by an integrated structural biology approach, including X-ray crystallography, single particle cryo-electron microscopy and small angle X-ray scattering. Surprisingly, it assembles into mixture of concentration-dependent dimers, tetramers and hexamers by dimer self-association. Hexameric particles form an unprecedented asymmetric horseshoe-like arrangement, which considerably differs from symmetric hexameric assembly found in many other protein structures. A single mutation within the self-association interface is sufficient to abolish any higher-level oligomerization, resulting in a homogenous dimeric assembly. The small C-terminal domain of yeast Oxalyl-CoA synthetase is connected by a partly flexible hinge with the large N-terminal domain, which provides the sole basis for oligomeric assembly. Our data provide a basis to mechanistically study peroxisomal translocation of this target.
Parametric simulation of stagnation point flow of motile microorganism hybrid nanofluid across a circular cylinder with sinusoidal radius
Authors: Raizah Zehba , Saeed Anwar , Bilal Muhammad , Galal Ahmed M. , Bonyah Ebenezer
Published: January 2023

The article explores the three-dimensional stream of silver (Ag), magnesium oxide (MgO), and motile microorganism water-based hybrid nanofluids as independent of time through a circular cylinder with a sinusoidal radius. The goal of this research is to optimize the rate of energy and mass transfer through a circular cylinder having a periodic radius. The phenomena are simulated as a system of partial differential equations containing momentum, temperature, concentration, and the profile of motile microbes, which were then simplified to a dimensionless system of ordinal differential equations using the similarity technique. The problem is solved by using the parametric continuation method, which is a numerical methodology. From the analysis, it has been perceived that both the energy and velocity fields significantly enhance with the rising effect of hybrid nanoparticles (Ag–MgO). The effect of chemical reaction enhances the mass transition rate because chemical reaction parameter influence exercises the molecules inside the fluid. The motile microorganism outline is elevated with the increment of Lewis and Peclet number.
Nanotechnology-based drug delivery for the treatment of CNS disorders
Authors: Mittal Khushi R. , Pharasi Nandini , Sarna Bhavya , Singh Manisha , Rachana  , Haider Shazia , Singh Sachin Kumar , Dua Kamal , Jha Saurabh Kumar , Dey Abhijit , Ojha Shreesh , Mani Shalini , Jha Niraj Kumar
Published: January 2023

Approximately 6.8 million people die annually because of problems related to the central nervous system (CNS), and out of them, approximately 1 million people are affected by neurodegenerative diseases that include Alzheimer’s disease, multiple sclerosis, epilepsy, and Parkinson’s disease. CNS problems are a primary concern because of the complexity of the brain. There are various drugs available to treat CNS disorders and overcome problems with toxicity, specificity, and delivery. Barriers like the blood–brain barrier (BBB) are a challenge, as they do not allow therapeutic drugs to cross and reach their target. Researchers have been searching for ways to allow drugs to pass through the BBB and reach the target sites. These problems highlight the need of nanotechnology to alter or manipulate various processes at the cellular level to achieve the desired attributes. Due to their nanosize, nanoparticles are able to pass through the BBB and are an effective alternative to drug administration and other approaches. Nanotechnology has the potential to improve treatment and diagnostic techniques for CNS disorders and facilitate effective drug transfer. With the aid of nanoengineering, drugs could be modified to perform functions like transference across the BBB, altering signaling pathways, targeting specific cells, effective gene transfer, and promoting regeneration and preservation of nerve cells. The involvement of a nanocarrier framework inside the delivery of several neurotherapeutic agents used in the treatment of neurological diseases is reviewed in this study.
Anticancer heterocyclic hybrids: design, synthesis, molecular docking and evaluation of new thiazolidinone-pyrazoles
Authors: Shrivastava Neelima , Khan Shah Alam , Alam Mohammad Mumtaz , Akhtar Mymoona , Srivastava Apeksha , Husain Asif
Published: January 2023

In order to obtain potential anticancer agents, hybrid compounds have been synthesized by coupling thiazolidinone and pyrazole scaffolds. Among the synthesized compounds, 2-(1,3-diphenyl-1H-pyrazol-4-yl)-3-phenyl thiazolidin-4-one (4a) was found to be the most potent based on a docking (−9.307) and binding scores (−66.46), along with good ADME parameters. In vitro anticancer activity of compound 4a shows a maximum inhibition against lung cancer (NCI-H23) cell lines with a moderate inhibition rate of 31.01%. Molecular docking studies revealed that these hybrid compounds bind well to the active site of peroxisome proliferator-activated receptors-gamma (PPAR-gamma). Doxorubicin was used as a positive control. It can be concluded that 4a having pyrazole-thiazolidinone ring systems has the potential to be developed as an anticancer agent.
Modified oligoether-diamine synthesis for the preparation of crystallizable polymers based on epoxyurethane oligomers
Authors: Slobodinyuk Alexey , Elchisheva Nadezhda , Strelnikov Vladimir , Chernova Galina , Slobodinyuk Daria
Published: January 2023

A modified synthetic method for amino-terminated oligo tetramethylene oxides is presented. Diamines were synthesized via a three-step route from oligo tetramethylene oxide diol with an average molecular weight of 2000. The final step – oligoether-diphthalimide hydrazinolysis – has been improved. The yield of the target product has been shown to be more than 1.5 times higher when the molar ratio of the reacting components was changed. The oligoether-diamine and the reaction intermediates have been identified by 1H and 13C NMR spectroscopy. It has been demonstrated that the synthesized amine can be used as a curing agent for epoxy urethane oligomers. It is shown that the glass transition temperature of the cured elastomers is lower than −70 °C. These elastomers can be recommended for the use in the conditions of the Arctic and the Far North or Far South of the globe.
Variación morfológica en semillas de Pinus pseudostrobus Lindl. altamente productores de resina
Authors: Muñoz Flores Hipólito Jesús , Sáenz Reyes J. Trinidad , Gómez Cárdenas Martín , Hernández Ramos Jonathan , BARRERA RUBEN
Published: January 2023

Los análisis de conos y semillas permiten evaluar las características físicas y biológicas de un lote de semillas y determinar la cantidad y calidad de semilla producida en unidades productoras de germoplasma forestal. El objetivo de este estudio fue determinar la variación morfológica y germinación de la semilla de 48 familias resineras de Pinus pseudostrobus Lindl., distribuidas en tres gradientes altitudinales en rodales naturales de la Comunidad Indígena de Nuevo San Juan Parangaricutiro (CINSJP), Michoacán. Se evaluaron las variables: ancho, largo, peso y germinación, número de semillas por kilogramo y contenido de humedad de la semilla. Los resultados mostraron diferencias significativas (p ≤ 0.05) entre gradientes y entre familias en las variables. El tamaño, peso de la semilla y porcentajes de germinación fueron mayores en los gradientes 1 y 2 (2200 m. s. n. m.-2400 m. s. n. m. y 2401 m. s. n. m.-2600 m. s. n. m.); en número de semillas existe diferencia estadística (p ≤ 0.05) entre familias en cada gradiente con 35 760 a 108 966 semillas (kg-1).
Canagliflozin Regulates Ferroptosis, Potentially via Activating AMPK/PGC-1α/Nrf2 Signaling in HFpEF Rats
Authors: Ma Sai , He Lili , Zuo Qingjuan , Zhang Guorui , Guo Yifang
Published: January 2023

Aims:Sodium-glucose cotransporter-2 (SGLT2) inhibitors have been found to ameliorate major adverse cardiovascular events in patients with heart failure with preserved ejection fraction (HFpEF), but the exact mechanism is unknown. Ferroptosis is a form of programmed necrosis. Herein, we verified that canagliflozin (CANA) ameliorates heart function in HFpEF rats, partly by regulating ferroptosis, which may be activated by AMPK/PGC-1α/Nrf2 signaling.

Methods:An HFpEF model was established and subjected to CANA treatment. Blood pressure was monitored, and echocardiography was performed at the 12thweek. Pathological examination was performed, and expression of ferroptosis-associated proteins and AMPK/PGC-1α/Nrf2 signaling related proteins was detected.

Results:CANA had an antihypertensive effect and increased E/A ratios in HFpEF rats. Myocardial pathology was ameliorated, on the basis of decreased cross-sectional area and intercellular fibrosis. Acyl-CoA synthetase long-chain family member 4 (ACSL4) expression increased, whereas ferritin heavy chain 1 (FTH1) expression decreased in HFpEF rats, which showed iron overload. CANA reversed changes in ACSL4 and FTH1, and decreased iron accumulation, but did not alter glutathione peroxidase 4 (GPX4) expression. The expression of AMPK/PGC-1α/Nrf2 signaling related proteins and heme oxygenase 1 (HO-1) in the HFpEF group decreased but was reverted after CANA treatment.

Conclusions:CANA regulates ferroptosis, potentially via activating AMPK/PGC-1α/Nrf2 signaling in HFpEF rats.
Microglia Maintain Homeostatic Conditions in the Developing Rostral Migratory Stream
Authors: Meller Sarah J. , Hernandez Lexie , Martin-Lopez Eduardo , Kloos Zachary A. , Liberia Teresa , Greer Charles A.
Published: January 2023

Microglia invade the neuroblast migratory corridor of the rostral migratory stream (RMS) early in development. The early postnatal RMS does not yet have the dense astrocyte and vascular scaffold that helps propel forward migrating neuroblasts, which led us to consider whether microglia help regulate conditions permissive to neuroblast migration in the RMS. GFP-labeled microglia inCX3CR-1GFP/+mice assemble primarily along the outer borders of the RMS during the first postnatal week, where they exhibit predominantly an ameboid morphology and associate with migrating neuroblasts. Microglia ablation for 3 d postnatally does not impact the density of pulse labeled BrdU+ neuroblasts nor the distance migrated by tdTomato electroporated neuroblasts in the RMS. However, microglia wrap DsRed-labeled neuroblasts in the RMS of P7CX3CR-1GFP/+;DCXDsRed/+mice and express the markers CD68, CLEC7A, MERTK, and IGF-1, suggesting active regulation in the developing RMS. Microglia depletion for 14 d postnatally further induced an accumulation of CC3+ DCX+ apoptotic neuroblasts in the RMS, a wider RMS and extended patency of the lateral ventricle extension in the olfactory bulb. These findings illustrate the importance of microglia in maintaining a healthy neuroblast population and an environment permissive to neuroblast migration in the early postnatal RMS.
Brain FNDC5/Irisin Expression in Patients and Mouse Models of Major Depression
Authors: Lima-Filho Ricardo , Fortuna Juliana S. , Cozachenco Danielle , Isaac Alinny R. , Lyra e Silva Natalia , Saldanha Alice , Santos Luis E. , Ferreira Sergio T. , Lourenco Mychael V. , De Felice Fernanda G.
Published: January 2023

Major depressive disorder (MDD) is a major cause of disability in adults. MDD is both a comorbidity and a risk factor for Alzheimer’s disease (AD), and regular physical exercise has been associated with reduced incidence and severity of MDD and AD. Irisin is an exercise-induced myokine derived from proteolytic processing of fibronectin type III domain-containing protein 5 (FNDC5). FNDC5/irisin is reduced in the brains of AD patients and mouse models. However, whether brain FNDC5/irisin expression is altered in depression remains elusive. Here, we investigate changes infndc5expression in postmortem brain tissue from MDD individuals and mouse models of depression. We found decreasedfndc5expression in the MDD prefrontal cortex, both with and without psychotic traits. We further demonstrate that the induction of depressive-like behavior in male mice by lipopolysaccharide decreasedfndc5expression in the frontal cortex, but not in the hippocampus. Conversely, chronic corticosterone administration increasedfndc5expression in the frontal cortex, but not in the hippocampus. Social isolation in mice did not result in alteredfndc5expression in either frontal cortex or hippocampus. Finally, fluoxetine, but not other antidepressants, increasedfndc5gene expression in the mouse frontal cortex. Results indicate a region-specific modulation offndc5in depressive-like behavior and by antidepressant in mice. Our finding of decreased prefrontal cortexfndc5expression in MDD individuals differs from results in mice, highlighting the importance of carefully interpreting observations in mice. The reduction infndc5mRNA suggests that decreased central FNDC5/irisin could comprise a shared pathologic mechanism between MDD and AD.
La teoría del framing aplicada al ‘rescate’ a España
Authors: Rodríguez Pérez Carlos
Published: January 2023

La teoría del framing estudia el enfoque que adoptan los mensajes y cómo se conceptualizan los hechos en una narrativa discursiva. Mediante el lenguaje los medios de comunicación encuadran la realidad con marcos que construyen el contenido mediante la selección y la prominencia de ciertos rasgos y atributos. Con ello definen lo que sabrá la gente e inciden en la formación de la opinión pública. Este artículo de investigación estudia cómo se comportaron los medios de comunicación durante el ‘rescate’ a España para determinar cuáles fueron los enfoques dominantes y la posición que adoptaron respecto al Gobierno.
El fenómeno del “spin doctoring” en España: el “juego” de la democracia entre los “spin doctor” y los periodistas
Authors: Cala Siria Mª Reyes
Published: January 2023

El fenómeno del “spin doctoring” pone su foco de atención en los “spin doctor” y los profesionales de los medios de comunicación. Según el profesor José Luis Dader, la acepción más certera para definir el verbo “to spin” es la del diccionario Webster que lo entiende como “formar una tela mediante la expulsión de un fluido viscoso rápidamente endurecido”. Esta idea implica entender esta actividad como un tejer a la red a los periodistas de manera sutil y subrepticia, mediante contactos informales sin acciones imperativas. En esta ponencia profundizo en la concepción que los protagonistas de esta actividad en España poseen de las reglas de “juego” que se establecen entre estos dos ámbitos y del nivel de democracia que existe entre sus interrelaciones.
¿Por qué las campañas? Las elecciones europeas de 2014 en España
Authors: Lagares Diez Nieves , López María Pereira , Jaráiz Gulías Erika
Published: January 2023

Los resultados de las elecciones europeas celebradas el pasado año supusieron importantes cambios para el sistema de partidos, pero también para el comportamiento electoral de los ciudadanos, y en consecuencia, para la construcción de los diferentes segmentos de votantes que apoyaron a las diferentes formaciones políticas.Una construcción en la cual la campaña electoral parece haber marcado ciertas diferencias, especialmente, para los partidos y/o coaliciones emergentes; que han visto en ella y en la propia lógica electoral de estos comicios, una estructura de oportunidad para la conformación de electorados que puedan permitirles competir en próximos contextos. Por ello, intentaremos desgranar en este trabajo el perfil de los diferentes grupos de votantes atendiendo no sólo a elementos clásicos, sino también y sobre todo, al contexto que supuso la campaña electoral.
La teoría del voto económico y la elección presidencial mexicana de 2012
Authors: Aguirre Manuel Ernesto Pérez
Published: January 2023

¿Qué factores explican el comportamiento del electorado mexicano?; desde lo particular, ¿la percepción económica influyó en el comportamiento del electorado mexicano en la elección presidencial de 2012? Los resultados del proyecto fueron inesperados. Al parecer, el electorado mexicano vota según perciba el desempeño gubernamental, bajo una lógica de premio o castigo al partido en el gobierno, pero lo hace desde un marco más amplio que el ofrecido por la teoría del voto económico. Sin embargo, el factor económico sí fue importante en el proceso de toma de decisiones del electorado mexicano durante la última elección presidencial.
Partidos políticos y competición electoral. Cuando la Ciencia Política confronta el Derecho
Authors: Fernández Luiña Eduardo , Fernández Luiña Eduardo , Trujillo Álvarez Pedro
Published: January 2023

La definición de partido político ha sido, desde la reconversión de la ciencia política en la segunda mitad del siglo XX, uno de los grandes temas de la disciplina. Dicha conceptualización ha contribuido al desarrollo de varias teorías dedicadas a interpretar, explicar el funcionamiento y clasificar los distintos sistemas de partidos. El esfuerzo intelectual de definición ha chocado con otro tipo de conceptualizaciones provenientes de disciplinas afines como el Derecho. Los motivos que han generado esta disociación entre definiciones politológicas y jurídicas podrían ser varios. Sin embargo, las consecuencias para el sistema político son muy claras. El trabajo presenta un estudio de caso sobre el sistema político guatemalteco y los problemas asociados a la conceptualización de la figura del partido político. Se observa que la definición jurídica eleva los costos de participación dificultando el acceso de los ciudadanos a las instituciones públicas. Además de presentar los elementos que las organizaciones guatemaltecas deben poseer para convertirse en partidos, el texto evidencia como dichos requisitos no sólo alzan los citados costes de participación, sino que reducen la competencia limitando la acción de actores alternativos, los denominados comités cívicos, que podrían pugnar presentando candidaturas distritales de naturaleza nacional y con ello contribuir a la dinamización de la estructura de competición electoral existente en el país.  La conclusión a la que llega nuestra investigación es: el sistema político guatemalteco presenta una clara disonancia entre la definición politológica de partido político y la definición jurídica que, inevitablemente, eleva los costos de participación y con ello limita la competencia a nivel general dentro del sistema.
Un esquema de la acción social. Aplicación a las reglas del fútbol
Authors: Allones Carlos
Published: January 2023

En este artículo presentamos en primer lugar al lector un esquema para explicar en general la acción humana colectiva, cualquiera que sea su contenido. El esquema, que fue originalmente desarrollado para analizar la Instrucción Militar (Allones, 1983), se presenta ahora en una versión más actual y avanzada, incluyendo su verificación empírica. Pero debido a su alcance universal, su terminología es necesariamente abstracta; por eso, para facilitar al lector y a nosotros mismos la comprensión de este esquema, acudiremos en segundo lugar a un ejemplo particular de acción social, muy conocido de todos y muy bien documentado: el fútbol. Aplicaremos nuestro esquema paso a paso al análisis del fútbol, para entender cómo se segregó a partir del rugby y cual fue su proceso de rápida y efectiva institucionalización en Inglaterra a lo largo del siglo XIX.
Periodic hygrothermal properties of Salix liquefaction products / isocyanate rigid foam materials
Authors: Wang Xuelin , Zhu Xiaofeng , Wang Bin , Zhang Minghui , An Zhen , Han Wang
Published: January 2023

Under specific circumstances, the liquefaction product of biomass polyol can be converted into hard polyurethane foam. In this study, the rigid foam material (RPUF) was created by reacting the liquefaction product of Salix psammophila with isocyanate. The mechanical properties, chemical structure, cell structure, and thermal stability of RPUF were characterized by mechanical properties tests, Fourier transform infrared (FTIR), scanning electron microscopy (SEM), and thermogravimetric analysis (TGA). The impact strength, compressive strength, and flexural strength of RPUF after hygrothermal treatment showed a downward trend. FTIR showed that the change of temperature and humidity did not affect the chemical structure of RPUF. SEM showed that the destruction of cell structure of RPUF was increased with the extension of time. TGA showed that the initial decomposition temperature of RPUF was reduced by periodic hydrothermal treatment, and the residual carbon rate decreased with the extension of treatment time.
Microelement imbalance in the rat uterus after short-term heavy metals exposure
Authors:   Sumy State University, Ukraine, Sikora K. , Lyndina Y. ,   Sumy State University, Ukraine, Awuah W.A. ,   Sumy State University, Ukraine, Abdul-Rahman T. ,   Sumy State University, Ukraine, Chyzhma R. ,   Sumy State University, Ukraine, Hyriavenko N. ,   Sumy State University, Ukraine, Lyndin M. ,   Sumy State University, Ukraine,   University of Duisburg-Essen, Germany, Sikora V. ,   Sumy State University, Ukraine,   University of Foggia, Italy, Romaniuk A. ,   Sumy State University, Ukraine
Published: January 2023

Heavy metals (HMs) are distinguishable among various exogenous elements with a comprehensive impact and high adverse environmental consequences. The danger of their spread is accompanied by risks of entry into living organisms, leading to organ accumulation, homeostasis disruption, and the development of various inflammatory and oncological clinical manifestations. Interestingly, the uterus shows an unpredictable reaction to the influence of these chemical elements, with almost all previous studies providing differing results. Therefore, this study investigated the features of chemical element content in the rat uterus after 30 days of HMs exposure and assessed the benefits of vitamin E against HMs impacts. Spectral analysis of uterine tissue of rats (daily HMs exposure) and animals (HMs exposure with vitamin E treatment) showed a significantly higher accumulation of Zn, Cu, Fe, Mn, Pb, and Cr after 30 days of the experiment. Although the uterus samples of rats treated with vitamin E showed a significant accumulation of HMs, it should be noted, that the tendency for their accumulation was less pronounced than in untreated rats. This study showed the accumulation features of some chemical elements in the rat uterus after 30 days of HMs exposure. Therefore, widespread environmental HMs exposure can leads to high absorption levels in uterine tissues. However, increases in HMs levels were significantly attenuated by vitamin E supplementation. Unfortunately, while a positive trend was found for each chemical element, the complete protection and purification effect of vitamin E use against HMs exposure was not observed.
DISTRIBUTION OF SERINE PROTEASES IN BLOOD PLASMA AND PANCREAS IN CHRONIC PANCREATITIS AND ONCOPATHOLOGY
Authors:   Taras Shevchenko National University of Kyiv, Educational and Scientific Centre Institute of Biology and Medicine, Ukraine, Synelnyk T.B. , Kravchenko O.O. ,   Taras Shevchenko National University of Kyiv, Educational and Scientific Centre Institute of Biology and Medicine, Ukraine, Kostiuk O.S. ,   Taras Shevchenko National University of Kyiv, Educational and Scientific Centre Institute of Biology and Medicine, Ukraine, Savchuk O.M. ,   Taras Shevchenko National University of Kyiv, Educational and Scientific Centre Institute of Biology and Medicine, Ukraine, Sukhodolia S.A. ,   National Pirogov Memorial Medical University of Vinnytsya, Ukraine, Ostapchenko L.I. ,   Taras Shevchenko National University of Kyiv, Educational and Scientific Centre Institute of Biology and Medicine, Ukraine
Published: January 2023

The aim of our study was to evaluate the trypsin-like serine proteases (TLPs) distribution between systemic circulation and pancreatic tissue and to investigate the peculiarities of their involvement in the extracellular matrix components degradation in patients with pancreatic pathologies with electrophoretic analysis methods using. Тhe Khmelnitsky Regional Clinical Hospital patients aged 28-89 were selected for this study: 20 people with chronic pancreatitis (group CP); 20 people with pancreatic cancer (group PC); 20 conditionally healthy persons (control). Blood plasma samples and pancreatic tissue homogenates were obtained from all the patients, from which the TLPs fractions were subsequently obtained by the affinity chromatography method. The study showed that TLPs content in the blood plasma of patients with pancreatic pathologies is higher, and in tissue homogenates is lower relative to the values of the corresponding indicators in the control. Disk-electrophoresis using showed that TLPs fractions obtained from the blood plasma of patients of all studied groups contain a lot of high molecular weight (HMW) proteins, while TLPs from the pancreatic tissue homogenates of patients with pancreatic pathologies mainly consists of low molecular weight (LMW) proteins. Enzyme-electrophoresis results showed that all TLPs fractions include enzymes with fibrinogenolytic, gelatinolytic and collagenolytic activity. In plasma, the first were represented by medium molecular weight (MMW) proteins, and the last two groups included a lot of HMW proteins as well as proteins with very high molecular weight. In homogenates, fibrinogenolytic activity was characteristic for LMW proteins only, whereas gelatinases and collagenases were represented by both MMW and LMW proteins. Our results indicate the differences in the TLPs fractions components obtained from blood plasma and pancreatic tissue of patients with investigated pathologies, as well as significant distinctions in the processes of extracellular matrix remodeling under СР and РС.
FUNCTIONAL ACTIVITY OF HEMOPOIETIC PROGENITOR CELLS FROM CORD BLOOD IN LONG-TERM CULTURE ON HYDROGEL SUBSTRATE EX VIVO
Authors:   National University of Kyiv-Mohyla Academy, Ukraine, Bilko D.I. , Dyagil I.S. ,   SI National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine, Ukraine
Published: January 2023

Development of new and improvement of existing models of long-term cultivation of hematopoietic stem cells (HSC) and cord blood progenitor cells is one of the most important directions in modern cell biology, related to obtaining a sufficient amount of hematopoietic tissue for experimental and clinical use. The aim of the work was to investigate the functional activity of hematopoietic progenitor cells from cord blood during long-term cultivation on a soft polyacrylamide gel substrate in the presence of a cytokine complex and to determine the advantages of this method of maintaining hematopoiesis for further use in transplantation. To realize this purpose, the following methods were used: the method of long-term cultivation in vitro on a hydrogel substrate, the method of colony formation in semi-liquid agar, cytological research methods, light and inverted microscopy, statistical research methods. Due to the use of the hydrogel substrate together with the cytokine complex, a high proliferative activity of hematopoietic cells is observed, which is reflected in their expansion during long-term cultivation (5 weeks), as well as of high colony-forming activity (521.5 ± 7.5.105 per explanted cells). Thus, the stiffness of the substrate must be taken into account for the expansion of stem cells and their immediate descendants. The presence of a soft substrate made of polyacrylamide gel along with a complex of cytokines ensures the expansion of hematopoietic cells due to the long- term support of hematopoiesis.
Deficiency and insufficiency of vitamin D in the Ukraine – update 2022
Authors:   State Institution “D.F. Chebotarev Institute of Gerontology of the NAMS of Ukraine”, Kyiv, Ukraine, Grygorieva N.V. , Musiienko A.S. ,   State Institution “D.F. Chebotarev Institute of Gerontology of the NAMS of Ukraine”, Kyiv, Ukraine, Bystrytska M. A. ,   State Institution “D.F. Chebotarev Institute of Gerontology of the NAMS of Ukraine”, Kyiv, Ukraine, Solonenko T.Yu. ,   State Institution “D.F. Chebotarev Institute of Gerontology of the NAMS of Ukraine”, Kyiv, Ukraine
Published: January 2023

Data from previous studies demonstrate the high frequency of deficiency and insufficiency of vitamin D in Ukraine, as in the world, which varies depending on the age and gender of the population, the season, the region of residence, and the type of concomitant pathology. The purpose of the study was to assess the vitamin D status in the Ukraine population during 2016-2022 years depending on age, sex, month, and year of observation. In a single-center cohort study, serum 25-hydroxyvitamin D (25(OH)D) level was analyzed in 7105 subjects aged 20-99 years. The analysis was performed depending on age, sex, month, and year of observation. The mean serum 25(OH)D level in the total group was 30.9 [22.1-41.0] ng/ml, the lowest level was in the age group 90-99 years old and the highest one was in the subjects aged 40-69 years old. 52.7% of the subjects had a sufficient vitamin D level, 27.4% had insufficiency, and 19.9% had a deficiency of vitamin D. No gender differences were found in the serum level of 25(OH)D, except the one for the women aged 60-69 years old, who had higher vitamin D levels compared to males parameters. Seasonal 25(OH)D levels variations indicated the highest values in September and October and the lowest ones in February and March. Additionally, we established the increase of serum 25(OH)D from 2016 to 2021 with the highest values in 2020 and 2021. Our data confirmed a decrease in vitamin D deficiency and insufficiency in 2021 and 2022 in the Ukrainian population compared to previous years (2016-2019) and previous studies in the Ukrainian population while maintaining their age-related and seasonal characteristics. It may be associated with an improvement in public awareness of global vitamin D deficiency and its positive skeletal and extraskeletal effects, as well as the COVID-19 pandemic in recent years.
Early Identification of Severe Sepsis in Pediatric Patients Using an Electronic Alert System
Authors: Oddiri Uchechi , Propper Grace , Brill Patricia , Reid Brienna , Giarraputo Dominic , Milana Carolyn
Published: January 2023

INTRODUCTION

Prompt sepsis recognition and the initiation of standardized treatment bundles lead to improved outcomes. We developed automated severe sepsis alerts through the electronic medical record and paging system to aid clinicians in rapidly identifying pediatric patients with severe sepsis in our emergency department and inpatient units. Our Specific, Measurable, Applicable, Realistic, Timely aim was to improve 1-hour severe sepsis treatment bundle compliance to 60% with these electronic interruptive alerts.

METHODS

We developed the alert’s criteria based on the 2005 International Pediatric Sepsis Consensus definitions. We performed 2 interventions: requiring the bedside nurse to answer the already implemented nurse-targeted (NT) severe sepsis alert, and the implementation of the physician-targeted (PT) severe sepsis alert. When systemic inflammatory response syndrome criteria were met, the NT alert triggered, and when organ dysfunction was also identified, an interruptive PT alert triggered, and the respective clinician was paged to evaluate the patient. Our primary outcome measure was bundle compliance; our secondary measure was PT alert response compliance.

RESULTS

Baseline severe sepsis treatment bundle compliance was 37%. After requiring nursing response to the NT alert in 2016 and implementing the PT alert in 2018, our bundle compliance rose to 69% in 2020, demonstrating statistically significant difference (P = .006). PT alert response compliance rose from 67% in 2018 to 91% in 2020.

CONCLUSIONS

An interruptive severe sepsis screening alert sent directly to clinicians is a valuable tool to ensure prompt severe sepsis recognition and treatment. This biphasic alert system facilitated multidisciplinary collaboration in early sepsis diagnosis and management.
Regionalitätskonzepte – Ungarische Literaturgeschichtsschreibung nach 1945 im Kontext der rumänischen Literaturgeschichte
Authors: Vincze Ferenc
Published: January 2023

In the discourse of 20th and 21st century Hungarian literary history writing the aspect of regionality is recurrently present, primarily in relation to minority/ethnic Hungarian literature. The positions and relations of these literatures can be inferred not only from the perspective of their naming and designation, but also from the way they are discussed or even represented as books. The study attempts to show the regionality formations in the history of Hungarian literature, which in mutual reflection, overlapping and displacement, have tried to gain a dominant position in the discourse on Hungarian literature. As a point of comparison, the essay also includes the regionality phenomena of Romanian literature in the field of interpretation.
Małżeństwo cywilne katolików – jego walor kanoniczny i możliwość uważnienia
Authors: Steczkowski Piotr
Published: January 2023

A civil marriage of Catholics was rethought in the Church doctrine only in context of the plague of, so called, ”free unions”. The reason of this was the fact that a civil marriage brings some legally binding duties. Canon law considers such marriage not as invalid, but as not existing. The marriage has not matrimonium putativum status, because of the lack of the form of the celebration of marriage, which is compulsory.The civil marriage cannot be validated in a simple way (sanatio simplex). The doctrine was confirmed recently by the Roman Rota. The marriage in question can be valid only in retroactive way (sanatio in radice), because the act of the competent authority includes required dispensation form canonical form of marriage.
Zebranie Synodu Biskupów według Regulaminu z 2006 roku
Authors: Rozktur Tomasz
Published: January 2023

Dopo aver terminato il Concilio Vaticano II, il Sinodo dei Vescovi rimane un’istituzione che, avendo le radici nel Concilio, forma l’immagine della Chiesa post-conciliare. Perciò uno dei più significativi contributi del Concilio Vaticano II alla struttura organizzativa e al sistema amministrativo della Chiesa è indubbiamente proprio il Sinodo dei Vescovi, ciò viene confermato dal periodo post- conciliare insieme alle riunioni di questa istituzione che sono state già ventitre. Contemporaneamente il Sinodo dei Vescovi ha svolto e svolge ancora un importante ruolo anche durante i pontificati dei papi post-conciliari, in particolare negli anni del lungo ponificato di Giovanni Paolo II (1978–2005), anche il pontificato attuale dimostra che il papa Benedetto XVI dà molta importanza a questa  istituzione convocando regolarmente il Sinodo dei Vescovi. Nell’articolo viene presentato il nuovo regolamento del Sinodo dei Vescovi (Ordo Synodi Episcoporum) approvato da Benedetto XVI nel 2006. Gli articoli del Regolamento del 2006 ci dimostrano che  abbiamo a che fare con un’istituzione nella Chiesa postconciliare, dal punto do vista giuridico, organizzata molto bene.
Ochrona tajemnicy spowiedzi. Zakaz wyjawiania okoliczności uciążliwych dla penitenta
Authors: Rakoczy Tomasz
Published: January 2023

The subject of the article is a norm of can. 984 § 1 that is expressed in statement: “A confessor is completely prohibited from using the knowledge acquired from confession to the detriment of the penitent even when any danger of revelation is excluded”. The basic problem of the article is the correct understanding of the sacramental seal. It embraces not only direct or indirect violation of the seal, but also refers to every circumstance that could be inconvenient for the penitent. The article shows arguments that might cause the departing from the ban. They are as follows: the care of a better celebration of the sacrament of penance, consultation with another confessor in case of the necessity, revelation of the sin for granting faculty and the dialogue with penitent about confession on penitent’s wish. The Roman congregations and blessed Pope John Paul II used to warn confessors against the unwise revelation of the knowledge acquired from confession. The problem is still serious and requires permanent care of the bishops,  including the use of the canonical penalties.
Znaczenie i rola wychowania religijnego w jednoczeniu wspólnoty rodzinnej
Authors: Jóżwik Michał
Published: January 2023

In Christian culture religious education is understood as supporting a person in their development and growth in religious maturity that is presented by Jesus the role model.Christian faith motivates a man to aim at integral and broad development that is achieved through self-discovery, self-evaluation and self-education.Family life in faith unites the whole family even more. Faith brings answers to the most important questions that dwell in a human being in his most significant matters related to the aim of life and its meaning. Faith sustains the parental authority weakening in  he eyes of a child, ordering respect towards parents but also towards a child.Depriving one of their family, of the supernatural values and rejecting Christ leads to the death of faith, to cutting it off the proper source from which faith dwells its life and spiritual power.The aim of this article is to attempt showing the role of religious upbringing in unifying a family according to the Christian concept of self-education and the teachings of John Paul II.
Przeszkody do przyjęcia i wykonywania święceń w Kodeksie Prawa Kanonicznego z 1983 roku
Authors: Zaborowski Marek
Published: January 2023

Der obige Artikel schildert die Problematik der Irregularitäten und Hindernisse zum Empfang und zur Ausübung der Weihen. Dieses Problem wird durch den gültigen Codex des kanonischen Rechts in Kanonen 1040–1046 geregelt. Diese Grundsätze haben den  Schutz und Beachtung der Heiligkeit und Würde des Weihesakraments zum Ziel. Die Kirche war seit den Zeiten der Apostel stets darum bemüht, dass sich die Priester durch solche Eigenschaften auszeichen, die ihrer großen Würde entsprechen würden. Auch heutzutagen ist die Sorge um eine entsprechende Geistesbildung der angehenden Priester eine der wichtigsten Fragen der Kirche.
Ks. Krzysztof Burczak, Sacrilegium w „Dekrecie” Gracjana, Lublin 2010, ss. 527
Authors: Rozkrut Tomasz
Published: January 2023

Recenzja publikacji: "Sacrilegium w Dekrecie Gracjana", Ks. Krzysztof Burczak
Piotr Skonieczny OP, La buona fama: problematiche inerenti alla sua protezione in base al can. 220 del Codice di diritto canonico latino, Roma 2010, ss. 352
Authors: Majer Piotr
Published: January 2023

Recenzja publikacji: "La buona fama: problematiche inerenti alla sua protezione in base al can. 220 del Codice di diritto canonico latino", Piotr Skonieczny OP
Julia Dickstein-Wieleżyńska e la storia editoriale della prima traduzione completa dei Canti in polacco
Authors:   Uniwersytet Marii Curie-Skłodowskiej w Lublinie, Polonia, Kłos Anita
Published: January 2023

The paper focuses on the long and complex editorial history of the first complete translation of Giacomo Leopardi’s Canti into Polish. Entitled Poezje and published by Instytut Wydawniczy ‘Biblioteka Polska’ in 1938, the volume was translated by Julia Dickstein-Wieleżyńska (1881–1948), a polyglot, poet, organiser of cultural events, feminist activist and literature and philosophy scholar. Dickstein-Wieleżyńska’s letters to Raffaele Pettazzoni (1883–1959), her close friend and mentor and himself an eminent scholar of religions, suggest that the original idea of the book as conceived in the early 1920s also envisaged the inclusion of translations by Edward Porębowicz (1862–1937), which had been made for his 1887 collection of Leopardi’s writings. Although Porębowicz had withdrawn his permission for publishing his versions in 1924 and Dickstein-Wielżyńska had to translate another eighteen poems, the manuscript was ready for printing at the beginning of 1925. Drawing on archival resources, the author investigates the reasons behind the conspicuous temporal distance between the drafting of the translations and their publication in the volume of Poezje. Examined from the perspective of recent translation and collaboration studies, Wieleżyńska’s letters shed some new light not only on the agents, modes and circumstances of translation and editorial work in the early twentieth century, but also on the position of women in the cultural and academic hierarchies of the time.
Divulgazione del sapere sulla natura e sull’ecologia: l’offerta editoriale della casa editrice Nasza Księgarnia
Authors:   Uniwersytet Wrocławski, Polonia, Jamróz-Stolarska Elżbieta
Published: January 2023

The article presents a quantitative and qualitative analysis of the repertoire of Nasza Księgarnia, a more than 100-year-old Polish publishing house specialising in children’s and youngadult literature, in terms of informational books on nature and ecology. The study covers the years 2011–2021, as a clear increase in the number of such publications was observable all over the world in this period. The main trends in the development of informational books published by Nasza Księgarnia, their dominant themes, and their prevalent genres are identified. Two books on endangered and threatened species are qualitatively analysed by means of Nina Goga’s method as illustrative of the releases and trends under study.
Libri per ragazzi: formazione della coscienza ecologica e trasformazioni correnti negli ambiti della non-fiction
Authors:   Università di Catania, Italia, Todaro Letterio , Mascia Tiziana ,   Università di Urbino, Italia
Published: January 2023

The article analyses children’s non-fiction aimed at developing ecological and environmental awareness, with a particular focus on Italian publishing production. On examining recent trends in the domain, it is possible to observe significant elements of change: an increasing focus on the use of captivating narrative techniques and a great attention to the visual element of the book, which functions both as an aesthetic element and as demonstrative evidence. Observably, communication in non-fiction is more and more premised on narrative approaches that cleverly stimulate imaginative and critical thinking skills. Overcoming the purely informative and scientific content-based communicative paradigm, traditionally associated with the notion of non-fiction, and integrating new forms of narrative writing, recent non-fiction exhibits an emotional-affective dimension capable of stimulating critical reflection, ecological awareness and virtuous behaviour for the protection of the environment in all its aspects.