bluuebunny/crossref_metadata_2025 huggingface dataset:
Superiority of proatrial natriuretic peptide in the prognostic power in patients with acute decompensated heart failure on hospital admission: comparison with B-type natriuretic peptide and other natriuretic peptide forms
Authors: Takashio Seiji , Takahama Hiroyuki , Nishikimi Toshio , Hayashi Tomohiro , Nagai-Okatani Chiaki , Matsuo Ayaka , Nakagawa Yasuaki , Amano Masashi , Hamatani Yasuhiro , Okada Atsushi , Amaki Makoto , Hasegawa Takuya , Kanzaki Hideaki , Yasuda Satoshi , Kangawa Kenji , Anzai Toshihisa , Minamino Naoto , Izumi Chisato
Published: July 2019

Aims

There are significant differences in how atrial (A-type) and B-type natriuretic peptide (ANP and BNP) are secreted and metabolised, but there is little information available about the relative clinical significance of the two peptides. The aim of the present study was to investigate: (1) the association between the circulating level of each ANP molecular form and patient clinical background and (2) their prognostic power for patients with acute decompensated heart failure (ADHF).

Methods

We used specific chemiluminescence enzyme immunoassays to prospectively evaluate the levels of six bioactive molecular forms of ANP (pro-ANP, β-ANP and total ANP) and BNP (pro-BNP, N-terminal pro-BNP (NT-pro-BNP) and total BNP) in plasma samples collected from 173 patients with ADHF on their hospital admission.

Results

We found that pro-ANP levels were strongly associated with left ventricular (LV) size and ejection fraction (p<0.001), but were not associated with left atrial size. Percent pro-ANP ([pro-ANP/total ANP]x100) was also associated with LV size and function. During the follow-up term (median: 469 days), composite adverse events (all causes of death or rehospitalisation for HF) occurred in 67 patients (38.7 %). Pro-ANP was significantly associated with composite adverse events even after adjusting by estimated glomerular filtration rate (eGFR) (p<0.05). In contrast, NT-pro-BNP was not independent of eGFR in the multivariate analysis.

Conclusion

Circulating levels of pro-ANP are strongly associated with LV function and clinical outcomes of patients with ADHF. These findings suggest that during the acute phases of HF, pro-ANP has a prognostic power comparable with NT-pro-BNP independently of renal function.
The association of waterpipe tobacco smoking with later initiation of cigarette smoking: a systematic review and meta-analysis exploring the gateway theory
Authors: Al Oweini Dana , Jawad Mohammed , Akl Elie A
Published: July 2019

Introduction

There is a concern that waterpipe tobacco smoking (WTS) can lead to the later initiation of cigarette smoking, a concept referred to as the ‘gateway theory’. The objective of the study was to systematically review the literature for the association of WTS with later initiation of cigarette smoking.

Methods

We searched Medline, Embase and ISI Web of Science in April 2018 without using any language or date restrictions. We selected eligible studies, abstracted data and assessed the risk of bias using a duplicate and independent approach. We meta-analysed the ORs across eligible studies using the inverse variance method and the random-effects model. We assessed the certainty of evidence using the Grading of Recommendations Assessment, Development and Evaluation methodology.

Results

We included eight papers reporting on six eligible prospective cohort studies with a total of 21 224 participants, belonging to the adolescent and young adult age categories. The pooled adjusted OR for the association between ever waterpipe user (compared with never waterpipe user) at baseline, with ‘cigarette smoking initiation’ (ever cigarette use) was 2.54 (95% CI 1.60 to 4.02) at 6 months to 3 years follow-up (moderate certainty evidence). The pooled adjusted OR for the association between ever waterpipe user (compared with never waterpipe user) at baseline, with ‘current cigarette smoking’ (past 30-day cigarette use) was 2.04 (95% CI 1.32 to 3.15) at 1–2 years follow-up (moderate certainty evidence). The pooled adjusted OR for the association between past 30-day waterpipe user (compared with never waterpipe user) at baseline, with current cigarette smoking (past 30-day cigarette use) 2.46 (95% CI 1.73 to 3.49) at 6 months to 1 year follow-up (high certainty evidence).

Conclusion

Our findings indicate that WTS is associated with more than doubling of the odds of later initiation of cigarette smoking, supporting the gateway theory. Strengthening WTS policies is a priority and further research is needed on the development and evaluation of appropriate clinical and public health interventions.
The effect of general duty police ensemble on graded exercise and simulated work performance
Authors: Ehnes Cameron Michael , Scarlett Michael Philip , Lemelin Sylvain Joseph , Stickland Michael Kenneth , Petersen Stewart Richard
Published: July 2019

This 2-part study examined the impact of general duty police ensemble on selected cardiopulmonary responses during incremental treadmill exercise and on simulated work performance in 25 healthy young male and female participants. Part I comprised randomly ordered treadmill tests in 2 experimental conditions: physical training (PT; undergarments, shorts, t-shirt, and running shoes) and police duty ensemble (PDE; undergarments, body armour, patrol uniform, boots, duty belt with required equipment, radio, and weapons). The PDE added 10.3 kg (SD 0.4) or 14% (SD 2) body mass. Participants walked at 5.6 km·h−1, starting at 0% grade with 2% increases in grade every 2 min. The 4% stage was 6 min in duration to achieve physiological steady state. Subsequently, the 2-min increments continued to exhaustion. Part II evaluated performance time on a recognized job-related work simulation circuit, in 3 experimental conditions: (i) PT, (ii) weighted belt (WB; PT plus a 7.5 kg weighted belt), and (iii) PDE. In Part I, physiological responses (e.g., oxygen uptake, ventilation, heart rate) were elevated (p < 0.05) with PDE during submaximal exercise but peak values were unchanged. Test duration and peak power output were significantly reduced with PDE. In Part II, circuit completion time was increased in PDE but not WB when compared with PT (p < 0.05). Heart rate and perceived exertion were similar in all conditions and perceived dyspnea was higher in PDE.

Novelty Police duty ensemble negatively affected exercise performance more than would be expected due to load mass alone. Specificity must be considered when simulating occupational load carriage.
Supply Chain Coordination with a Risk-Averse Retailer and a Combined Buy-Back and Revenue Sharing Contract
Authors: Zhao Han , Song Shiji , Zhang Yuli , Gupta Jatinder N. D. , Devlin Anna G. , Chiong Raymond
Published: July 2019

This paper investigates the ability of a combined buy-back (BB) and revenue sharing (RS) contract to improve the efficiency of a supply chain involving a risk-neutral supplier and a risk-averse retailer facing stochastic demand. We show that the combined contract can coordinate the supply chain under mild conditions. Further, the effects of risk aversion and contract parameters on the agents’ decision-making are analyzed when the retailer’s risk aversion is modeled by the conditional value-at-risk (CVaR) criterion. In contrast to individual BB and RS contracts, the combined contract is able to mitigate the effect of risk-aversion and allow the supplier to obtain higher expected profit. Moreover, situations exist where the combined contract can coordinate the supply chain when neither the BB nor the RS contract can coordinate it. Numerical experiments conducted further confirm the analytical results derived.
Bohmian mechanics of Klein–Gordon equation via quantum metric and mass
Authors: Jalalzadeh Shahram , Capistrano A. J. S.
Published: July 2019

The causal stochastic interpretation of relativistic quantum mechanics has the problems of superluminal velocities, motion backward in time and the incorrect non-relativistic limit. In this paper, according to the original ideas of de Broglie, Bohm and Takabayasi, we introduce simultaneously a quantum mass and a quantum metric of a curved spacetime to obtain a correct relativistic theory free of mentioned problems.
Computational investigations of MnNiCuSb alloy for structural, elasto-mechanical and vibrational properties
Authors: Bakar Abu , Afzal Maaz , Bashir Muzaffar , Afaq A. , Iftikhar Aneeza , Aziz Fahad , Asif Muhammad
Published: July 2019

The MnNiCuSb Quaternary Heusler Alloy (QHA) in Y-type I stable structure (cubic) is studied for structural, elastic, mechanical and vibrational properties by using Density Functional Theory (DFT). Three independent elastic constants [Formula: see text], [Formula: see text] and [Formula: see text] for this cubic system are computed with Generalized Gradient Approximation (GGA) functional. The mechanical parameters like Young’s modulus, Shear and Bulk modulus, Pugh’s ratio, Poisson’s ratio, anisotropic factor, Cauchy pressure are then calculated by using these cubic elastic parameters. In addition, phonon dispersion curve and phonon density of states (PDOS) are computed with norm-conserving Martins–Troullier pseudo-potential in Perturbed Density Functional Theory. The phonon dispersion curve provides reststrahlen band 0.727 THz [Formula: see text] for which material behaves as 100%. This value corresponds to Far Infra-Red (FIR) spectral region so this alloy can be used for manufacturing FIR-devices.
Stiff matter solution in Brans–Dicke theory and the general relativity limit
Authors: Brando G. , Fabris J. C. , Falciano F. T. , Galkina Olesya
Published: July 2019

Generally the Brans–Dicke (BD) theory reduces to general relativity (GR) in the limit [Formula: see text] if the scalar field goes as [Formula: see text]. However, it is also known that there are examples with [Formula: see text] that does not tend to GR. We discuss another case: a homogeneous and isotropic universe filled with stiff matter. The power of time dependence of these solutions does not depend on [Formula: see text], and there is no GR limit even though we have [Formula: see text]. A perturbative and a dynamical system analysis of this exotic case are carried out.
Nonlinear Static and Dynamic Analysis of Rocking Masonry Corners Using Rigid Macro-Block Modeling
Authors: Casapulla Claudia , Giresini Linda , Argiento Luca Umberto , Maione Alessandra
Published: July 2019

The corner failure is one of the most typical local mechanisms in masonry buildings vulnerable to earthquakes. The seismic assessment of this mechanism is poorly studied in the literature and in this paper it is addressed by means of both nonlinear static and dynamic analyses of rocking rigid blocks. The static approach is based on the displacement-based method and is aimed at predicting the onset of the 3D failure mechanism and its evolution through incremental kinematic analysis. This approach also considers the presence of a thrusting roof and the stabilizing contribution of frictional resistances exerted within interlocked walls. The capacity in terms of both forces and displacements is compared with the seismic demand through the construction of acceleration–displacement response spectra, with some originality. The nonlinear dynamic approach is based on the seminal Housner’s work on rocking rigid blocks and considers the influence of transverse walls, roof overloads and outward thrust, all included in an updated equation of one-sided motion. In particular, the process of defining an equivalent prismatic block, representative of the original corner geometry, is presented to convert the 3D dynamic problem into a 2D rocking motion. The wide suitability and advantage of such modeling approaches to assess the seismic response of rocking masonry structures with reference to specific limit states are demonstrated through a real case study, i.e. the collapse of a corner in a masonry school building during the 2016–2017 Central Italy seismic sequence. The compared results provide a good agreement of predictions in terms of both onset and overturning conditions, for which the static model appears to be more conservative than the dynamic one.
Some algebraic and homological properties of a family of quotients of the Rees algebra
Authors: Salek Mahnaz , Tavasoli Elham , Tehranian Abolfazl , Salimi Maryam
Published: July 2019

Let [Formula: see text] be a commutative ring and let [Formula: see text] be a proper ideal of [Formula: see text]. In this paper, we study some algebraic and homological properties of a family of rings [Formula: see text], with [Formula: see text], that are obtained as quotients of the Rees algebra associated with the ring [Formula: see text] and the ideal [Formula: see text]. Specially, we study when [Formula: see text] is a von Neumann regular ring, a semisimple ring and a Gaussian ring. Also, we study the classical global and weak global dimensions of [Formula: see text]. Finally, we investigate some homological properties of [Formula: see text]-modules and we show that [Formula: see text] and [Formula: see text] are Gorenstein projective [Formula: see text]-modules, provided some special conditions.
Derivations and automorphisms of nilpotent evolution algebras with maximal nilindex
Authors: Mukhamedov Farrukh , Khakimov Otabek , Omirov Bakhrom , Qaralleh Izzat
Published: July 2019

This paper is devoted to the nilpotent finite-dimensional evolution algebras [Formula: see text] with [Formula: see text]. We describe the Lie algebra of derivations of these algebras. Moreover, in terms of these Lie algebras, we fully construct nilpotent evolution algebra with maximal index of nilpotency. Furthermore, this result allowed us fully characterize all local and 2-local derivations of the considered evolution algebras. Besides, all automorphisms and local automorphisms of these algebras are found.
Group gradations on Leavitt path algebras
Authors: Nystedt Patrik , Öinert Johan
Published: July 2019

Given a directed graph [Formula: see text] and an associative unital ring [Formula: see text] one may define the Leavitt path algebra with coefficients in [Formula: see text], denoted by [Formula: see text]. For an arbitrary group [Formula: see text], [Formula: see text] can be viewed as a [Formula: see text]-graded ring. In this paper, we show that [Formula: see text] is always nearly epsilon-strongly [Formula: see text]-graded. We also show that if [Formula: see text] is finite, then [Formula: see text] is epsilon-strongly [Formula: see text]-graded. We present a new proof of Hazrat’s characterization of strongly [Formula: see text]-graded Leavitt path algebras, when [Formula: see text] is finite. Moreover, if [Formula: see text] is row-finite and has no source, then we show that [Formula: see text] is strongly [Formula: see text]-graded if and only if [Formula: see text] has no sink. We also use a result concerning Frobenius epsilon-strongly [Formula: see text]-graded rings, where [Formula: see text] is finite, to obtain criteria which ensure that [Formula: see text] is Frobenius over its identity component.
Liftings for ultra-modulation spaces, and one-parameter groups of Gevrey-type pseudo-differential operators
Authors: Abdeljawad Ahmed , Coriasco Sandro , Toft Joachim
Published: July 2019

We deduce one-parameter group properties for pseudo-differential operators [Formula: see text], where [Formula: see text] belongs to the class [Formula: see text] of certain Gevrey symbols. We use this to show that there are pseudo-differential operators [Formula: see text] and [Formula: see text] which are inverses to each other, where [Formula: see text] and [Formula: see text]. We apply these results to deduce lifting property for modulation spaces and construct explicit isomorphisms between them. For each weight functions [Formula: see text] moderated by GRS submultiplicative weights, we prove that the Toeplitz operator (or localization operator) [Formula: see text] is an isomorphism from [Formula: see text] to [Formula: see text] for every [Formula: see text].
Evaluating the Agile-Stage-Gate Hybrid Model: Experiences From Three SME Manufacturing Firms
Authors: Edwards Kasper , Cooper Robert G. , Vedsmand Tomas , Nardelli Giulia
Published: July 2019

Agile-Stage-Gate is a hybrid product development model that integrates elements of both Agile and Stage-Gate to help companies realize the strengths of both. Recent studies show positive results in manufacturing companies, although SMEs are notably absent despite being the majority. This paper reports results of a test of the model in three deliberately chosen manufacturing SMEs. Results were improved: time to market, overall new product process, higher success rate. Agile required adaptations, and novel solutions were found by the test firms. The positive results suggest that Agile-Stage-Gate must be considered as a recommended product development approach in SME manufacturers.
Tailored porphyrin–gold nanoparticles for biomedical applications
Authors: Grigore Madalina E. , Ion Rodica-M. , Iancu Lorena , Grigorescu Ramona M.
Published: July 2019

In this review we present an updated survey of the main synthesis methods of gold nanoparticles (AuNPs) in order to obtain various tailored nanosystems for biomedical imaging. The synthesis approach significantly impacts on the AuNPs properties such as surface chemistry, biocompatibility and cytotoxicity. In recent years, nanomedicine emphasized the development of functionalized AuNPs for biomedical imaging. AuNPs are a good option for used as delivery photosensitizer agents for PDT of cancer. For example, the complex formed from AuNPs functionalized with PEGylate porphyrins presents several advantages in the medical field such as a better use in photodynamic therapy because of high triplet states and singlet oxygen quantum yield efficiency of porphyrin molecules.
Dynamical Response of Atherosclerotic Plaque Through Mathematical Model
Authors: Mukherjee Debasmita , Guin Lakshmi Narayan , Chakravarty Santabrata
Published: July 2019

The present paper concerns itself with the dynamical response of the formation of atherosclerotic plaque arising from the interactions among various cellular components in arteries. An appropriate mathematical model is framed to articulate the involvement of several key cellular components like LDLs, HDLs, radicals, oxidized LDLs, chemo-attractants, etc. in terms of a large system of nonlinear ordinary differential equations. The present dynamical model bears the potential to have global stability and hence it furnishes a comprehensible essence for in silico studies on the onset of atherosclerotic plaque. Quasi steady state approximation (QSSA) theory is inducted for reduction of the larger system to a smaller one compliant with an in-depth study regarding its stability and bifurcation analytically. Special emphasis is put on the significant role of arterial radius in the process of disposition of atherosclerotic plaque by contending with the variability of wall shear stress (WSS). The applicability of the model is validated through numerically computed results based on existing parameter values so as to ascertain the implications of the biochemical process of arteriosclerotic plaque formation.
In-Silico Prediction on the MSAMS-Assisted Immobilization of Bovine Serum Albumin on 10MHz Piezoelectric Immunosensors
Authors: Urdinola Kaory Barrientos , Muñoz Paula Andrea Marín , Marín Pedronel Araque , Grajales Marisol Jaramillo
Published: July 2019

The biological sensing interface on the active area of a piezo transducer is responsible for the sensitivity, specificity, reusability, and reproducibility of these devices. Among the approaches used to functionalize piezo transducers, mixed self-assembled monolayers (MSAMs) are one of the most successful, given that they allow the obtaining of semi-crystalline molecular arrays and the arrangement of a bioreceptor on the surface. But, to deploy MSAMs on a substrate effectively, one must optimize and characterize the structural ratio between them and the bioreceptor. In this paper, we developed a molecular model of the interaction between Bovine Serum Albumin (BSA) and MSAMs-functionalized gold substrates. First, we evaluated the conditions for the functionalization of the substrates and found that a 50:1 16-mercaptohexadecaonic acid (MHDA) to 11 mercapto-1-undecanol (MUA) ratio produced the best features on the surface. We also evaluated the specific conditions to immobilize BSA on MSAMs (using the afore-established ratio) via Atomic Force Microscopy (AFM), and then on a 10[Formula: see text]MHz quartz crystal microbalance (QCM), and with the data obtained we concluded that a structural ratio of 0.005 (MSAM/BSA) is obtained when 1[Formula: see text][Formula: see text]M MHDA and 200[Formula: see text][Formula: see text]g/mL BSA were used, provided the most suitable conditions for the functionalization of a piezo transducer.
Sedimentary and diapiric mélanges in the Skrzydlna area (Outer Carpathians of Poland) as indicators of basinal and structural evolution
Authors: Siemińska Aneta , Starzec Krzysztof , Waśkowska Anna , Wendorff Marek
Published: July 2019

The Dukla Nappe in the Skrzydlna area exposes two types of mélange reflecting two different phases of basinal and tectonic evolution of the Outer West Carpathian orogen in its Polish sector. The Oligocene-age sedimentary mélange (olistostrome) is related to growth of the accretionary wedge, whereas the Miocene-age diapiric mélange postdates the orogenic thrusting. Textural and structural features of the very coarse-grained sedimentary mélange suggest non-cohesive debris flows and high-density turbidity currents as predominant emplacement mechanisms. Growth strata, associated with progressive unconformities, and facies contrast between the underlying fine-grained unit and the overlying olistostrome reflect a considerable uplift of the source area and rotation of the adjacent part of the basin floor. The olistostrome and the overlying turbidite succession form a retrogressive sequence interpreted as a submarine canyon infill grading to a small submarine fan. The diapiric mélange, injected into the Oligocene-age succession of the Dukla Nappe, contains the Early and Late Cretaceous-age blocks and matrix derived from the underlying Silesian Nappe. The features reflecting diapiric emplacement include matrix proportion increase and block content decrease towards the mélange margins, scaly fabric and shear zones. Both mélanges, interpreted in the past as chaotic bodies, upon detailed examination reveal genesis-related subtle internal organization.
Enclosing Chebyshev Expansions in Linear Time
Authors: Hashemi Behnam
Published: July 2019

We consider the problem of computing rigorous enclosures for polynomials represented in the Chebyshev basis. Our aim is to compare and develop algorithms with a linear complexity in terms of the polynomial degree. A first category of methods relies on a direct interval evaluation of the given Chebyshev expansion in which Chebyshev polynomials are bounded, e.g., with a divide-and-conquer strategy. Our main category of methods that are based on the Clenshaw recurrence includes interval Clenshaw with defect correction (ICDC), and the spectral transformation of Clenshaw recurrence rewritten as a discrete dynamical system. An extension of the barycentric representation to interval arithmetic is also considered that has a log-linear complexity as it takes advantage of a verified discrete cosine transform. We compare different methods and provide illustrative numerical experiments. In particular, our eigenvalue-based methods are interesting for bounding the range of high-degree interval polynomials. Some of the methods rigorously compute narrow enclosures for high-degree Chebyshev expansions at thousands of points in a few seconds on an average computer. We also illustrate how to employ our methods as an automatic
a posteriori
forward error analysis tool to monitor the accuracy of the Chebfun feval command.
An Exploration of the Drivers of Non-Adoption Behavior
Authors: Wolverton Colleen Carraher , Cenfetelli Ronald
Published: July 2019

While there has been a substantial amount of attention within the information systems research community towards understanding the phenomenon of adoption, much less is known about non-adoption. This study examines the factors surrounding the decision to not adopt a technology and whether certain factors exert differing effects on individuals in particular ways such that concurrent factors could be identified to develop a classification of the specific types of non-adoption behavior. Utilizing inhibitor theory and the symbolic adoption model as a foundational framework for the different types of non-adoption, we posit that different types of non-adoption exist which is demonstrated by determining the perceptions towards technology that coalesce around different types of non-adoption. We conducted a two-phase investigation into non-adoption with two goals in mind: 1) identify and explore specific factors of the IT that are associated with the rejection decision and are distinct from the adoption decision, and 2) determine the extent to which these factors (along with traditional enablers) differentiate between different types of non-adoption. The results from a discriminant function analysis (DFA) indicate the coalescence of specific perceptual variables according to the types of non-adoption behavior, specifically, the discriminatory power of differing perceptions of IT between trial rejecters, symbolic rejecters, trial accepters, symbolic adopters, and adopters. The implications for research and implications for practice are discussed.
He Said, She Said
Authors: Riemenschneider Cynthia K. , Buche Mari W. , Armstrong Deborah J.
Published: July 2019

The preponderance of the academic research focused on diversity in the IS field has emphasized the perspectives of women and racioethnic minorities. Recent research has found that following the appointment of a female CEO, white male top managers provided less help to colleagues, particularly those identified as minority-status (McDonald, Keeves, & Westphal, 2018). Additionally, Collison and Hearn (1994) assert that white men's universal status and their occupancy of the normative standard state have rendered them invisible as objects of analysis. To develop a more holistic view of the IS workplace, we expand the academic exploration by looking at the challenges men face in the Information Systems (IS) workplace. Using a cognitive lens, we evoke the challenges men perceive they face at work and cast them into revealed causal maps. We then repeat the process evoking women's perspectives of men's challenges. The findings are analyzed using the Communication Theory of Identity (CTI) to determine the areas of overlap and identity gaps. This study advances our understanding of the cognitive overlap (and lack thereof) regarding the challenges facing men in the IS field, and provides another step toward developing a more inclusive IS work environment.
How much do preclinical medical students utilize the internet to study physiology?
Authors: O’Malley Dervla , Barry Denis S. , Rae Mark G.
Published: July 2019

Medical students increasingly utilize social media platforms to supplement their preclinical learning; however, the prevalence of social media use for physiology learning in medical education remains unclear. The aim of the present study was to determine how first-year medical students from both direct entry medicine and graduate entry medicine interacted with social media as a learning tool by assessing its prevalence, perceived benefits, favored platforms, and reason(s) for its use. Seventy-one percent of surveyed students (out of 139 participants) stated that they interacted with social media in general more than 12 times per week. However, 98% had previously used internet platforms to source physiology information, with 89.2% doing so at least once per week during term. YouTube was the primary source of learning for 76% of students. Significantly, 94% of students indicated that they would first search for answers online if they did not understand something in physiology rather than contacting their instructor in person or by e-mail. However, only 31% of students “fact-checked” physiology information obtained from online sources, by using textbooks, papers, and/or instructors. Our study has revealed that most preclinical medical students utilize social media extensively to study physiology. However, the absence of academic and ethical oversight, paired with students’ lack of critical appraisal of possibly inaccurate information, does raise concerns about the overall utility of social media as part of physiology education.
GPR120 controls neonatal brown adipose tissue thermogenic induction
Authors: Quesada-López Tania , Gavaldà-Navarro Aleix , Morón-Ros Samantha , Campderrós Laura , Iglesias Roser , Giralt Marta , Villarroya Francesc
Published: July 2019

Adaptive induction of thermogenesis in brown adipose tissue (BAT) is essential for the survival of mammals after birth. We show here that G protein-coupled receptor protein 120 (GPR120) expression is dramatically induced after birth in mouse BAT. GPR120 expression in neonatal BAT is the highest among GPR120-expressing tissues in the mouse at any developmental stage tested. The induction of GPR120 in neonatal BAT is caused by postnatal thermal stress rather than by the initiation of suckling. GPR120-null neonates were found to be relatively intolerant to cold: close to one-third did not survive at 21°C, but all such pups survived at 25°C. Heat production in BAT was significantly impaired in GPR120-null pups. Deficiency in GPR120 did not modify brown adipocyte morphology or the anatomical architecture of BAT, as assessed by electron microscopy, but instead impaired the expression of uncoupling protein-1 and the fatty acid oxidation capacity of neonatal BAT. Moreover, GPR120 deficiency impaired fibroblast growth factor 21 (FGF21) gene expression in BAT and reduced plasma FGF21 levels. These results indicate that GPR120 is essential for neonatal adaptive thermogenesis.
eNOS deletion impairs mitochondrial quality control and exacerbates Western diet-induced NASH
Authors: Sheldon Ryan D. , Meers Grace M. , Morris E. Matthew , Linden Melissa A. , Cunningham Rory P. , Ibdah Jamal A. , Thyfault John P. , Laughlin M. Harold , Rector R. Scott
Published: July 2019

Dysregulated mitochondrial quality control leads to mitochondrial functional impairments that are central to the development and progression of hepatic steatosis to nonalcoholic steatohepatitis (NASH). Here, we identify hepatocellular localized endothelial nitric oxide synthase (eNOS) as a novel master regulator of mitochondrial quality control. Mice lacking eNOS were more susceptible to Western diet-induced hepatic inflammation and fibrosis in conjunction with decreased markers of mitochondrial biogenesis and turnover. The hepatocyte-specific influence was verified via magnetic activated cell sorting purified primary hepatocytes and in vitro siRNA-induced knockdown of eNOS. Hepatic mitochondria from eNOS knockout mice revealed decreased markers of mitochondrial biogenesis (PPARγ coactivator-1α, mitochondrial transcription factor A) and autophagy/mitophagy [BCL-2-interacting protein-3 (BNIP3), 1A/1B light chain 3B (LC3)], suggesting decreased mitochondrial turnover rate. eNOS knockout in primary hepatocytes exhibited reduced fatty acid oxidation capacity and were unable to mount a normal BNIP3 response to a mitophagic challenge compared with wild-type mice. Finally, we demonstrate that eNOS is required in primary hepatocytes to induce activation of the stress-responsive transcription factor nuclear factor erythroid 2-related factor 2 ( NRF2). Thus, our data demonstrate that eNOS is an important regulator of hepatic mitochondrial content and function and NASH susceptibility.
Early Treatment with Empagliflozin and GABA Improves β-Cell Mass and Glucose Tolerance in Streptozotocin-Treated Mice
Authors: Daems Caroline , Welsch Sophie , Boughaleb Hasnae , Vanderroost Juliette , Robert Annie , Sokal Etienne , Lysy Philippe A.
Published: July 2019

While the autoimmune character of T1D (type 1 diabetes) is being challenged, it is currently recognized that inflammation plays a key role in its development. We hypothesized that glucotoxicity could contribute to β-cell mass destruction through participation in islet inflammation. We evaluated the potential of empagliflozin (EMPA) and GABA (gamma-aminobutyric acid) to protect β-cell mass against glucotoxicity and to increase β-cell mass after diagnosis of T1D. Empagliflozin is a SGLT2 (sodium-dependent glucose cotransporter) inhibitor which thereby blocks glucose recapture by the kidney and promotes glucose excretion in urine. GABA is an inhibitory neurotransmitter, which stimulates α-to-β cell transdifferentiation. In streptozotocin-treated mice, empagliflozin and/or GABA were delivered for a period of five days or three weeks. As compared to untreated T1D mice, EMPA-treated T1D mice had decreased FFA (free fatty acid) levels and improved glucose homeostasis. EMPA-treated T1D mice had higher islet density, with preserved architecture, compared to T1D mice, and EMPA-treated T1D mice also differed from T1D mice by the total absence of immune cell infiltration within islets. Islets from EMPA-treated mice were also less subjected to ER (endoplasmic reticulum) stress and inflammation, as shown by qPCR analysis. Glucose homeostasis parameters and islet area/pancreas area ratio improved, as compared to diabetic controls, when T1D mice were treated for three weeks with GABA and EMPA. T1D EMPA+GABA mice had higher glucagon levels than T1D mice, without modifications of glucagon area/islet area ratios. In conclusion, empagliflozin and GABA, used in monotherapy in streptozotocin-induced diabetic mice, have positive effects on β-cell mass preservation or proliferation through an indirect effect on islet cell inflammation and ER stress. Further research is mandatory to evaluate whether empagliflozin and GABA may be a potential therapeutic target for the protection of β-cell mass after new-onset T1D.
Genetics of Stay-Green Trait and Its Association with Leaf Spot Tolerance and Pod Yield in Groundnut
Authors: Danful Rukiya , Kassim Yussif Baba , Puozaa Doris Kanvenaa , Oteng-Frimpong Richard , Rasheed Masawudu Abdul , Wireko-Kena Alexander , Akromah Richard
Published: July 2019

Despite its importance in providing income and food for smallholder farmers, fodder for livestock, and improving soil fertility through biological nitrogen fixation, groundnut yields are lowest on farmers’ fields in Sub-Saharan Africa due to biotic and abiotic constraints. Foliar fungal diseases account for over 80% reduction in groundnut productivity in some parts of Ghana. Unfortunately, chemical control of these foliar diseases has not yielded the desired results. Meanwhile, advances in phenotyping for disease tolerance in other crops have established a strong relationship between stay-green trait and foliar disease tolerance. However, this relationship has not been explored in groundnut. This study was designed to determine the genetic control of the stay-green trait and its relationship with leaf spot disease severity in groundnut. Twenty-five advanced groundnut breeding lines with varying degrees of tolerance for leaf spot tolerance were evaluated under diseased and disease-free conditions, after which four were selected for genetic studies. Results showed significant (p<0.001) differences among the genotypes for early leaf spot (ELS), late leaf spot (LLS), leaf area under greenness (LAUG), SPAD chlorophyll meter readings (SCMR), and yield traits. Leaf spot diseases caused 4.95 t·ha−1 (64.54%) pod yield reduction in CHINESE, the widely cultivated groundnut variety in Ghana. There was a strong correlation between LAUG and ELS (r = 0.82, p<0.001) and LLS (r = 0.63, p<0.001), and genotypes that were stay-green had tolerance to both diseases. Stay-green trait in groundnut was detected to be under the control of a single recessive gene and hence may be used to select for ELS and LLS resistance.
The Antiobesity Effects of Buginawa in 3T3-L1 Preadipocytes and in a Mouse Model of High-Fat Diet-Induced Obesity
Authors: Park Yea-Jin , Seo Dong-Wook , Ju Jae-Yun , Cha Yun-Yeop , An Hyo-Jin
Published: July 2019

There has been a remarkable interest in finding lipid inhibitors from natural products to replace synthetic compounds, and a variety of oriental medicinal herbs are reported to have biological activity with regard to lipid inhibition. Buginawa (Bugi) is a novel combined formula that contains twelve medicinal herbs with potential for weight loss induction. We hypothesized that Bugi may have antiobesity effects in 3T3-L1 preadipocytes and in a high-fat diet- (HFD-) induced mouse model. In this study, 3T3-L1 cells were treated with varied concentrations of Bugi (62.5, 125, or 250 μg/mL). Bugi treatment inhibited adipocyte differentiation by suppressing adipogenic transcription genes, including peroxisome proliferator-activated receptor γ protein (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), sterol regulatory element-binding protein 1 (SREBP1), and CCAAT/enhancer-binding protein β (C/EBPβ). Mice were fed a normal diet or an HFD for 11 weeks, and Bugi was simultaneously administered at 50 or 100 mg/kg. Bugi administration significantly reduced body weight gain and white adipose tissue (WAT) weight and effectively inhibited lipid droplet accumulation in epididymal white adipose tissue (eWAT) and liver tissue. Further, Bugi treatment suppressed mRNA levels of PPARγ, C/EBPα, and SREBP1 in eWAT and liver tissue. Our findings demonstrate that Bugi could be an effective candidate for preventing obesity and related metabolic disorders.
A Review on Optimal Placement of Sensors for Cooperative Localization of AUVs
Authors: Bo Xu , Razzaqi Asghar A. , Farid Ghulam
Published: July 2019

Self-positioning of submerged Autonomous Underwater Vehicles (AUVs) is a challenging task due to nonavailability of GPS signals. One of the most recent solutions for this is the use of surface vehicles (sensors) for cooperative localization of the underwater vehicles (targets) by measuring their relative positions. However, correct placement of the surface sensors is very critical as their geometric configuration affects their observability and hence availability of their relative positions information to the targets. In this paper, a comparative survey of sensors’ optimal formation techniques for cooperative localization of AUVs has been presented. Introduction to the basic cooperative localization techniques and background theory of optimal sensor placements have been provided. This paper can also serve as a fundamental reading material for students and researchers pursuing research on optimal sensor placement for cooperative localization.
The Increased lncRNA MIR503HG in Preeclampsia Modulated Trophoblast Cell Proliferation, Invasion, and Migration via Regulating Matrix Metalloproteinases and NF-κB Signaling
Authors: Cheng Dan , Jiang Shan , Chen Jiao , Li Jie , Ao Liangfei , Zhang Ying
Published: July 2019

Background. Preeclampsia (PE) is a pregnancy-related syndrome characterized by hypertension and proteinuria after the 20th week of gestation. The long noncoding RNAs (lncRNAs) have been recently discovered for their roles in the pathogenesis of PE. This study is aimed at determining the expression of lncRNA MIR503 host gene (MIR503HG) in PE placental tissues and exploring the molecular mechanism underlying MIR503HG-mediated trophoblast cell proliferation, invasion, and migration. Methods. The expression level of MIR503HG in placental tissues, HTR-8/SVneo, and JEG3 cells was determined by quantitative real-time PCR; western blot detected the relevant protein expression levels in HTR-8/SVneo and JEG3 cells; flow cytometry determined cell apoptosis and cell cycle of HTR-8/SVneo and JEG3 cells; trophoblast cell proliferation, invasion, and migration of HTR-8/SVneo and JEG3 cells were measured by CCK-8, transwell invasion, and wound healing assays, respectively. Results. The highly expressed MIR503HG was detected in PE placental tissues compared to normal placental tissues. MIR503HG overexpression suppressed cell proliferation, invasion, and migration of HTR-8/SVneo and JEG3 cells, while knockdown of MIR503HG increased trophoblast cell proliferation, invasion, and migration. Flow cytometry results showed that MIR503HG overexpression induced apoptosis and caused cell cycle arrest at the G0/G1 phase, while MIR503HG knockdown had the opposite actions in HTR-8/SVneo and JEG3 cells. Western blot assay results showed that MIR503HG overexpression suppressed the matrix metalloproteinase-2/-9 and the snail protein expression and increased the E-cadherin expression in trophoblast cells. In addition, MIR503HG overexpression suppressed the NF-κB signaling pathway by inhibiting the phosphorylation of IκBα and the nuclear translocation of NF-κB signaling subunit p65. On the other hand, MIR503HG knockdown played an opposite role in these protein expression levels. Conclusion. Our results showed that MIR503HG inhibited the proliferation, invasion, and migration of HTR-8/SVneo and JEG3 cells, which may be related to the pathogenesis of PE.
Peptides Fixing Industrial Textile Dyes: A New Biochemical Method in Wastewater Treatment
Authors: Mosbah Amor , Chouchane Habib , Abdelwahed Soukaina , Redissi Alaeddine , Hamdi Manel , Kouidhi Soumaya , Neifar Mohamed , Slaheddine Masmoudi Ahmed , Cherif Ameur , Mnif Wissem
Published: July 2019

The aim of the present work was the development of a new biological method for the treatment of textile industry effluents, which is cheaper, more profitable, and eco-friendly. This method is essentially based on the synthesis of dye-fixing peptides. The use of peptides synthesized via a solid-phase synthesis to fix a reference textile dye like “Cibacron blue” (CB) and the performance analysis of binding assays were the main objectives of this study. For this reason, two peptides P1 (NH2-C-G-G-W-R-S-Q-N-Q-G-NH2) and P2 (NH2-C-G-G-R-R-Y-Q-P-D-S-NH2) binding with the CB dye were synthesized by the solid-phase peptide synthesis (SPPS) technique. The obtained results showed significant fixation yields of CB-peptides of 91.5% and 45.9%, respectively, and consequently, their interesting potential as a tool for a new biochemical method in the pollution prevention of textile wastewater.
Prevalence, Species Distribution, and Related Factors of Fish-Borne Trematode Infection in Ninh Binh Province, Vietnam
Authors: Tran Anh Kieu Thi , Doan Hoa Thuy , Do Anh Ngoc , Nguyen Van Thi , Hoang Su Xuan , Le Huong Thu Thi , Hoang Hoa Thi , Le Nam Hoang , Le Quyen Bao Thi , Le Tran-Anh
Published: July 2019

Background. Clonorchis sinensis/Opisthorchis viverrini and minute intestinal flukes (MIF) such as Haplorchis pumilio and H. taichui are fish-borne trematodes (FBT) that may coexist in regions where local people have a habit of eating raw fish like Vietnam. Responses to FBT should be verified according to the data on the distribution of these flukes. This study aims to explore the prevalence of different species of FBT and related factors among local people in a northern province of Vietnam. Methods. A cross-sectional study was conducted in Kim Son and Yen Khanh districts, Ninh Binh province, between March 2016 and March 2017. Four hundred people aged 15 years or older were interviewed and gave stool samples. The FBT eggs in faecal samples were enumerated by modified formalin-ether technique and identified by sequencing of the second internal transcribed spacer (ITS2) region. Result. Among the 400 persons, 19.5% were infected with FBT. On univariate analysis, eating raw fish was the main risk factor (odds ratios (OR)) of 6.769 (95% confidence interval (CI) of 2.655–17.259) followed by being of male gender (3.994 (CI95% 2.117–7.536)) and drinking alcohol (2.680 (CI95% 1.440–4.986)), respectively. There was no risk of increased infection among those living at home without hygienic latrines, those living close to rivers or having ponds, or those raising cats or dogs. By multivariate analysis, FBT infection was only related to the consumption of raw fish and gender. Seventy stool samples with a sufficient amount of faecal matter were subjected to DNA extraction, 42.85% of them yielded DNA production, and all were of Clonorchis sinensis. Conclusion. Results of the study showed the high prevalence of infection of fish-borne trematode, mostly C. sinensis among humans in Ninh Binh province. The prevention of FBT should be strengthened with programs detailed according to the distribution of FBT in different endemic areas.
Improving Cognitive Function after Traumatic Brain Injury: A Clinical Trial on the Potential Use of the Semi-Immersive Virtual Reality
Authors: De Luca Rosaria , Maggio Maria Grazia , Maresca Giuseppa , Latella Desiree , Cannavò Antonino , Sciarrone Francesca , Lo Voi Emanuele , Accorinti Maria , Bramanti Placido , Calabrò Rocco Salvatore
Published: July 2019

Traumatic brain injury (TBI) is the most common cause of long-term disability and death among young adults, and it represents an enormous socioeconomic and healthcare burden. Our purpose is to evaluate the effects of a virtual reality training with BTs-Nirvana (BTs-N) on the recovery of cognitive functions in TBI subjects, using the interactive semi-immersive program. One hundred patients with TBI were enrolled in this study and randomized into either the Traditional Cognitive Rehabilitation Group (TCRG: n=50) or the Virtual Reality Training Group (VRTG: n=50). The VRTG underwent a VRT with BTs-N, whereas the TCRG received a standard cognitive treatment. Each treatment session lasted 60 minutes and was repeated three times a week for 8 weeks. All of the patients were evaluated by a specific psychometric battery before (T0) and immediately (T1) after the end of the training. VRTG and TCRG had a significant improvement in cognitive functioning and in mood, but only VRTG presented with a significant increase in cognitive flexibility and shifting skills and in selective attention. In conclusion, our results suggest that VR may be a useful and effective approach for the rehabilitation of patients with TBI, leading to better cognitive and behavioral outcomes.
Research on the Characteristics of Infrared Radiation Spectra at Zigong Acupoint within Menstrual Cycle of Healthy Female
Authors: Qin Meng , Ma Si-qi , Huang Zhi-ming , Shen Xue-yong , Wei Jian-zi
Published: July 2019

The purpose of this study was to observe the characteristics of infrared radiation spectrum of Zigong acupoint (EX-CA1) within the menstrual cycle of healthy female. We used highly sensitive infrared radiation spectrum detection system and phase-locked amplification technology to detect and analyse the infrared radiation spectrum from 1.5μm to 18μm of 32 healthy female before, during, and after menstruation at EX-CA1 and control points. The results showed that the total radiation intensity of left EX-CA1 was significantly higher than that of left control point (P<0.05) at the whole menstrual cycle, and the difference between right EX-CA1 and right control points was statistically significant before and after menstruation (P<0.05), no statistical difference during menstruation. Previous studies found that the radiation near 15μm was correlated with glucose metabolism. The results of this study showed that there were statistical differences in 10 wavelengths between left EX-CA1 and left control point from 14μm to 18μm, and there were statistical differences in 3 wavelengths on the right side (P<0.05). The left side is more prominent than the right side. The infrared radiation intensity of EX-CA1 decreased gradually with the change of cycle rhythm within menstrual cycle, but there was no statistical difference in this trend. There was no statistical difference in total radiation intensity between the right and left side of EX-CA1. Compared with the control points, the number of different wavelengths between left EX-CA1 and left control point during menstruation was significantly larger than that between right EX-CA1 and right control point (P<0.001). The results indicated that the energy of EX-CA1 was higher than control points. There was no difference in the radiation intensity between the right and left sides of EX-CA1 but there was acupoint laterality compared with nonacupoints. There was no significant rhythmic change in infrared radiation intensity of EX-CA1 during the menstrual cycle.