bluuebunny/crossref_metadata_2025 huggingface dataset:
Generation of the radial higher-order orbital angular momentum mode in helically twisted elliptic fiber
Authors: Huang Chuangrong , Zhang Jie , Tu Jiajing , Gao Shecheng , Liu Weiping , Li Zhaohui
Published: September 2024

The generation mechanism and regulation method of the radial higher-order orbital angular momentum (OAM) mode based on helically twisted elliptic fiber (HTEF) are investigated. This work aims to reveal the quantitative relation between the twisted rate (α) and the target radial higher-order OAM mode. From theoretical simulations, it is concluded that α is the key to regulating the radial higher-order OAM mode. In the experiment, OAM2n modes with radial higher-order n of 2, 3, 4, and 5 are successfully generated at 1550 nm by regulating α of the HTEF. Finally, the transmission spectrum and purity measurements are performed for the OAM24 mode, and it can be concluded that the fabricated experimental samples have more than 90% conversion efficiency and close to 94% purity under the appropriate α and twisted length.
Laser-produced plasma water-window x-ray source by continuous liquid bismuth jet
Authors: Soramoto Tatsuya , Ogiwara Ayaka , Morita Hiroki , Jiang Weihua , Sakaue Kazuyuki , Ejima Takeo , O’Sullivan Gerry , Namba Shinichi , Higashiguchi Takeshi
Published: September 2024

We have demonstrated a continuous-operated water-window (WW) x-ray source using a regenerative liquid bismuth (Bi) jet for 120 min. A regenerative liquid Bi jet with a diameter of 35−40 µm was continuously injected into a vacuum. The number of photons per pulse was observed to be 3 × 1011 photons/sr and 4.2 × 1011 photons/sr at peak wavelengths of 4.1 nm and 4.3 nm, respectively, which arises from n = 4−n = 4 (Δn = 0) transitions, and 1.7 × 1011 photons/sr at a peak wavelength of 2.8 nm, which is attributed to n = 4−n = 5 (Δn = 1) transitions. The total number of photons emitted/pulse in the 2.3−4.4 nm range was 1012−1013 photons/(nm · sr) for 120 min. We also observed that the fast on spectra scales with the laser intensity according to the power of 0.4 of the laser intensity. These results suggest that the continuous regenerative liquid Bi jet operation could credibly provide future shorter wavelength extreme ultraviolet (EUV) lithography and x-ray microscopy source for biological imaging applications.
Beyond the 95s: What happens when uniform program targets are applied across a heterogenous HIV epidemic in Eastern and Southern Africa?
Authors: Joseph Rachael H. , Obeng-Aduasare Yaa , Achia Thomas , Agedew Abraham , Jonnalagadda Sasi , Katana Abraham , Odoyo Elijah J. , Appolonia Aoko , Raizes Elliot , Dubois Amy , Blandford John , Nganga Lucy
Published: September 2024

The UNAIDS 95-95-95 targets are an important metric for guiding national HIV programs and measuring progress towards ending the HIV epidemic as a public health threat by 2030. Nevertheless, as proportional targets, the outcome of reaching the 95-95-95 targets will vary greatly across, and within, countries owing to the geographic diversity of the HIV epidemic. Countries and subnational units with a higher initial prevalence and number of people living with HIV (PLHIV) will remain with a larger number and higher prevalence of virally unsuppressed PLHIV—persons who may experience excess morbidity and mortality and can transmit the virus to others. Reliance on achievement of uniform proportional targets as a measure of program success can potentially mislead resource allocation and progress towards equitable epidemic control. More granular surveillance information on the HIV epidemic is required to effectively calibrate strategies and intensity of HIV programs across geographies and address current and projected health disparities that may undermine efforts to reach and sustain HIV epidemic control even after the 95 targets are achieved.
Clinical presentation of human leptospirosis in febrile patients: Urabá, Colombia
Authors: Uribe-Restrepo Pablo , Perez-Garcia Janeth , Arboleda Margarita , Munoz-Zanzi Claudia , Agudelo-Florez Piedad
Published: September 2024

Background

Leptospirosis is responsible for various clinical syndromes, classically linked with fever and acute kidney injury.

Methodology/Principal findings

A prospective multicenter observational study was conducted in six health institutions in the region of Urabá, Colombia. Enrollment was based on leptospirosis-compatible clinical syndrome and a positive preliminary serological test, with PCR used to confirm the disease. Clinical data were collected using a standard questionnaire at enrollment, complemented with a review of clinical records. A total of 100 patients were enrolled, 37% (95% CI 27.0–46.9%) had a positive PCR result confirming acute leptospirosis. The most frequent symptoms in patients with a positive PCR test were headache (91.9%; 34/37), chills and sweating (80.6%; 29/37), nausea (75%; 27/37), dizziness (74.3%; 26/37), vomiting (61.1%; 22/37), congestion (56.8%; 21/37), and conjunctival suffusion (51.4%; 19/37). The frequency of clinical signs classically described in leptospirosis was low: jaundice (8.3%; 3/36) and anuria/oliguria (21.6%; 8/37). An increased neutrophile percentage was reported in 60.6% (20/33) of patients. The presence of complications was 21.6% (8/37), with pulmonary complications being the most frequent (75.0% 6/8). One confirmed case died resulting in a fatality of 2.7% (95% CI 0.5–13.8).

Conclusions/Significance

Leptospirosis should be considered within the differential diagnoses of an undifferentiated acute febrile syndrome. Leptospirosis presents diagnostic challenges due to limitations in both clinical and laboratory diagnosis thus it is important to improve understanding of disease presentation and identify signs and symptoms that might help differentiate it from other causes of febrile illness.
Metagenomic next generation sequencing of plasma RNA for diagnosis of unexplained, acute febrile illness in Uganda
Authors: Kandathil Abraham J. , Blair Paul W. , Lu Jennifer , Anantharam Raghavendran , Kobba Kenneth , Robinson Matthew L. , Alharthi Sultanah , Ndawula Edgar C. , Dumler J. Stephen , Kakooza Francis , Lamorde Mohammed , Thomas David L. , Salzberg Steven L. , Manabe Yukari C.
Published: September 2024

Metagenomic next generation metagenomic sequencing (mNGS) has proven to be a useful tool in the diagnosis and identification of novel human pathogens and pathogens not identified on routine clinical microbiologic tests. In this study, we applied mNGS to characterize plasma RNA isolated from 42 study participants with unexplained acute febrile illness (AFI) admitted to tertiary referral hospitals in Mubende and Arua, Uganda. Study participants were selected based on clinical criteria suggestive of viral infection (i.e., thrombocytopenia, leukopenia). The study population had a median age of 28 years (IQR:24 to 38.5) and median platelet count of 114 x103 cells/mm3 (IQR:66,500 to 189,800). An average of 25 million 100 bp reads were generated per sample. We identified strong signals from diverse virus, bacteria, fungi, or parasites in 10 (23.8%) of the study participants. These included well recognized pathogens like Helicobacter pylori, human herpes virus-8, Plasmodium falciparum, Neisseria gonorrhoeae, and Rickettsia conorii. We further confirmed Rickettsia conorii infection, the cause of Mediterranean Spotted Fever (MSF), using PCR assays and Sanger sequencing. mNGS was a useful addition for detection of otherwise undetected pathogens and well-recognized non-pathogens. This is the first report to describe the molecular confirmation of a hospitalized case of MSF in sub-Saharan Africa (SSA). Further studies are needed to determine the utility of mNGS for disease surveillance in similar settings.
The Ithildin library for efficient numerical solution of anisotropic reaction-diffusion problems in excitable media
Authors: Kabus Desmond , Cloet Marie , Zemlin Christian , Bernus Olivier , Dierckx Hans
Published: September 2024

Ithildin is an open-source library and framework for efficient parallelized simulations of excitable media, written in the C++ programming language. It uses parallelization on multiple CPU processors via the message passing interface (MPI). We demonstrate the library’s versatility through a series of simulations in the context of the monodomain description of cardiac electrophysiology, including the S1S2 protocol, spiral break-up, and spiral waves in ventricular geometry. Our work demonstrates the power of Ithildin as a tool for studying complex wave patterns in cardiac tissue and its potential to inform future experimental and theoretical studies. We publish our full code with this paper in the name of open science.
Trends in smoking initiation and cessation over a century in two Australian cohorts
Authors: James Alan L. , Caliskan Gulser , Pesce Giancarlo , Accordini Simone , Abramson Michael J. , Bui Dinh , Musk Arthur W. , Knuiman Matthew W. , Perret Jennifer L. , Jarvis Deborah , Minelli Cosetta , Calciano Lucia , Hui Jennie , Hunter Michael , Thomas Paul S. , Walters E. Haydn , Garcia-Aymerich Judith , Dharmage Shyamali C. , Marcon Alessandro
Published: September 2024

Background

Historical data on smoking can enhance our comprehension of the effectiveness of past tobacco control policies and play a key role in developing targeted public health interventions. This study was undertaken to assess trends in smoking initiation and cessation in Australia for the period 1910–2005.

Methods

Rates of smoking initiation and cessation were calculated for participants in two population-based cohorts, the Busselton Health Study and the Tasmanian Longitudinal Health Study. The effects of time trends, gender and age group were evaluated.

Results

Of the 29,971 participants, 56.8% ever smoked. In males, over the period 1910–1999, the rate of smoking initiation in young adolescents remained high with a peak in the 1970s; in older adolescents it peaked in the 1940s and then declined; in young adults it showed a steady decline. In females, the rate of smoking initiation in young adolescents rose sharply in the 1960s and peaked in the 1970s, in older adolescents it increased throughout the period, and in young adults it declined after 1970. In the period 1930–2005, 27.3% of 9,605 people aged 36–50 years who smoked ceased smoking. Rates of cessation in this age group increased throughout but decreased in males after 1990 and plateaued around 2000 in females.

Conclusion

Our findings show substantial variation in the efficacy of tobacco control policies across age groups, with a notable lack of success among the younger population.
Over twenty years of publications in Ecology: Over-contribution of women reveals a new dimension of gender bias
Authors: Fontanarrosa Gabriela , Zarbá Lucía , Aschero Valeria , Dos Santos Daniel Andrés , Nuñez Montellano María Gabriela , Plaza Behr Maia C. , Schroeder Natalia , Lomáscolo Silvia Beatriz , Fanjul María Elisa , Monmany Garzia A. Carolina , Alvarez Marisa , Novillo Agustina , Lorenzo Pisarello María José , D’Almeida Romina Elisa , Valoy Mariana , Ramírez-Mejía Andrés Felipe , Rodríguez Daniela , Reynaga Celina , Sandoval Salinas María Leonor , Chillo Verónica , Piquer-Rodríguez María
Published: September 2024

Biographical features like social and economic status, ethnicity, sexuality, care roles, and gender unfairly disadvantage individuals within academia. Authorship patterns should reflect the social dimension behind the publishing process and co-authorship dynamics. To detect potential gender biases in the authorship of papers and examine the extent of women’s contribution in terms of the substantial volume of scientific production in Ecology, we surveyed papers from the top-ranked journal Ecology from 1999 to 2021. We developed a Women’s Contribution Index (WCI) to measure gender-based individual contributions. Considering gender, allocation in the author list, and the total number of authors, the WCI calculates the sum of each woman’s contribution per paper. We compared the WCI with women’s expected contributions in a non-gender-biased scenario. Overall, women account for 30% of authors of Ecology, yet their contribution to papers is higher than expected by chance (i.e., over-contribution). Additionally, by comparing the WCI with an equivalent Men’s Contribution Index, we found that women consistently have higher contributions compared to men. We also observed a temporal trend of increasing women’s authorship and mixed-gender papers. This suggests some progress in addressing gender bias in the field of ecology. However, we emphasize the need for a better understanding of the pattern of over-contribution, which may partially stem from the phenomenon of over-compensation. In this context, women might need to outperform men to be perceived and evaluated as equals. The WCI provides a valuable tool for quantifying individual contributions and understanding gender biases in academic publishing. Moreover, the index could be customized to suit the specific question of interest. It serves to uncover a previously non-quantified type of bias (over-contribution) that, we argue, is the response to the inequitable structure of the scientific system, leading to differences in the roles of individuals within a scientific publishing team.
Hospital discharge processes: Insights from patients, caregivers, and staff in an Australian healthcare setting
Authors: Omonaiye Olumuyiwa , Ward-Stockham Kristel , Darzins Peteris , Kitt Clinton , Newnham Evan , Taylor Nicholas F , Considine Julie
Published: September 2024

Hospital discharge is a pivotal point in healthcare delivery, impacting patient outcomes and resource utilisation. Ineffective discharge processes contribute to unplanned hospital readmissions. This study explored hospital discharge process from the perspectives of patients, caregivers, and healthcare staff. Qualitative data were collected through semi-structured interviews with adult patients being discharged home from a medical ward, their caregivers, and healthcare staff at an Australian hospital. Thematic analysis followed established guidelines for qualitative research. A total of 65 interviews and 21 structured observations were completed. There were three themes: i) Communication, ii) System Pressure, and iii) Continuing Care. The theme ‘Communication’ highlighted challenges and inconsistencies in notifying patients, caregivers, and staff about discharge plans, leading to patient stress and frustration. Information overload during discharge hindered patient comprehension and satisfaction. Staff identified communication gaps between teams, resulting in uncertainty regarding discharge logistics. The theme ‘System Pressure’ referred to pressure to discharge patients quickly to free hospital capacity occasionally, even in the face of inadequate service provision on weekends and out-of-hours. The ‘Continuing Care’ theme drew attention to gaps in patient understanding of follow-up appointments, underscoring the need for clearer post-discharge instructions. The lack of structured systems for tracking referrals and post-discharge care coordination was also highlighted, potentially leading to fragmented care. The findings resonate with international literature and the current emphasis in Australia on improving communication during care transitions. Furthermore, the study highlights the tension between patient-centred care and health service pressure for bed availability, resulting in perceptions of premature discharges and unplanned readmissions. It underscores the need for strengthening community-based support and systems for tracking referrals to improve care continuity. These findings have implications for patient experience and safety and suggest the need for targeted interventions to optimise the discharge process.
Impact of occupational stress on healthcare workers’ family members before and during COVID-19: A systematic review
Authors: Tekin Sahra , Nicholls Helen , Lamb Dannielle , Glover Naomi , Billings Jo
Published: September 2024

We aimed to explore the experiences, needs, and mental health impact of family members of healthcare workers (HCWs) before and during the COVID-19 pandemic. Eleven quantitative and nine qualitative studies were included in this review. Based on the narrative synthesis, we identified five outcomes: ‘Mental health outcomes’, ‘Family relationships, ‘Coping skills and resilience’, ‘Quality of life and social life’, and ‘Practical outcomes’. Our findings indicated that there was a high risk to the mental health and well-being of families of healthcare workers both before and during the pandemic. However, with the COVID-19 pandemic, some experiences and mental health issues of families were worsened. There was also a negative association between working long hours/shift work and family relationships/communication, family social life, and joint activities, and family members taking on more domestic responsibilities. Families tended to use both positive and negative coping strategies to deal with their loved one’s job stress. Organisations and support services working with people in health care work should consider widening support to families where possible. With this understanding, HCWs and their families could be supported more effectively in clinical and organisational settings.

Trial registration: Systematic Review Registration Number: CRD42022310729. https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022310729.
Congenital transmission of Chagas disease: The role of newborn therapy on the disease’s dynamics
Authors: Boukaabar Meriem , Oduro Bismark , Chataa Paul
Published: September 2024

Chagas disease, also known as American trypanosomiasis, is caused by a protozoan blood-borne pathogen called Trypanosoma cruzi. The World Health Organization (WHO) has classified Chagas as one of 21 neglected tropical diseases present in the world and estimates that 6-7 million people are currently infected with Chagas. Congenital transmission of Chagas disease contributes to a significant amount of new infections, especially in endemic areas where 22.5% of new infections are due to congenital transmission. In this paper, we investigate congenital transmission’s impact on Chagas disease dynamics through a mathematical model. Specifically, we examine how treating a proportion of infants born to infected individuals impacts the progression and spread of Chagas disease. The influence of newborn therapy on the dynamics of the model is thoroughly investigated, both theoretically and numerically. The results illustrate the importance of treating a high proportion of newborns to reduce the number of infected cases of the disease. The findings show that the therapy given to newborns is necessary but not sufficient to curb the transmission of Chagas disease, and a comprehensive approach that includes vector and vertical transmission control strategies is essential for eradicating Chagas disease. We also observed that if vector transmission can be controlled, then at least 55% of the newborns need to be treated to eliminate the disease.
Why am I obsessed with viewing mukbang ASMR? The roles of mediated voyeurism and intertemporal choice
Authors: Jiang Nan , Khong Kok Wei , Chen Mobai , Khoo Kim Leng , Xavier Jesrina Ann , Jambulingam Manimekalai
Published: September 2024

This study investigates the determinants of the obsessive view of mukbang autonomous sensory meridian response (Mukbang ASMR) and examines the moderation role of intertemporal choice and the mediating effect of mediated voyeurism among university students in Malaysia. A quantitative survey was conducted with 408 university students in Malaysia who viewed mukbang channel(s) often on social media. PLS-SEM is adopted to examine the associated paths and effects. The results demonstrate the significant impact of alienation and novelty, vicarious satisfaction, companionship and loneliness on mukbang ASMR obsession. Mediated voyeurism intervenes the effects of alienation and novelty on mukbang ASMR and intertemporal choice positively moderates the relationship between companionship and loneliness and mukbang ASMR. This study constructs a model to estimate Mukbang ASMR obsession by identifying specific motives and relationships among key factors, highlighting loneliness as the most effective determinant of mukbang ASMR among Malaysian younger generation. Research results provide an extended understanding of the mukbang ASMR, offering valuable insights in the areas of lifestyle, social well-being, and social media consumption.
Willingness to pay for sheep traits and their heterogeneous effects on prices: Evidence from primary livestock markets in Ethiopia
Authors: Yitayew Asresu , Kassie Girma T. , Abduali Awudu , Nigussie Zerihun
Published: September 2024

We test the hypothesis whether levels of key traits of sheep heterogeneously affect market prices of sheep in a rural setting. Feasible generalized least squares and (un)conditional quantile regression estimations were made on a dataset of 1153 sheep transactions in two primary small ruminant markets in the Amhara region of Ethiopia. The empirical results show that animal traits affect the observed prices of sheep differently, but only partly explain the sheep price differences. Our results also reveal that in addition to animal traits, the type of buyers and seasonality of sheep marketing cause heterogeneity in the observed prices. These findings imply that targeting the animal traits demanded by the market and access to price information that enables farmers to respond to the seasonal changes in livestock markets are essential to increase the income of sheep keepers.
Epistatic interactions between oxytocin- and dopamine-related genes and trust
Authors: Koyama Yuna , Nawa Nobutoshi , Ochi Manami , Surkan Pamela J. , Fujiwara Takeo
Published: September 2024

Trust is an essential human trait. Although research suggests that the interplay between oxytocinergic and dopaminergic systems affects trust formation, little research has focused on epistatic (i.e., gene by gene) interaction effects of oxytocin- and dopamine-related genes on trust. Using a sample of 348 adults (114 men), we aimed to investigate the associations between genetic variants in oxytocin- and dopamine-related genes and the general, neighborhood, and institutional trust with consideration of sex differences. Three-way interaction between oxytocin-related gene genotypes, dopamine-related genotypes, and sex was found for the oxytocin receptor gene (OXTR)rs1042778 and the Catechol-O-Methyltransferase gene (COMT) rs4680 genotypes (p = 0.02) and for OXTR rs2254298 and the dopamine D2 receptor gene (DRD2) rs1800497 genotypes (p = 0.01). Further sex-stratified analyses revealed that the interaction between OXTR rs1042778 and COMT rs4680 genotypes was associated with neighborhood trust among men (p = 0.0007). Also, the interaction between OXTR rs2254298 and DRD2 rs1800497 genotypes was associated with institutional trust among men (p = 0.005). Post-hoc analyses found that men with OXTR rs1042778 TG/TT and COMT rs4680 GG genotypes reported higher neighborhood trust than those with GG + AG/AA (B = 13.49, SE = 4.68, p = 0.02), TG/TT + AG/AA (B = 23.00, SE = 5.99, p = 0.001), and GG + GG (B = 18.53, SE = 5.25, p = 0.003). Similarly, men with OXTR rs2254298 AG/AA and DRD2 rs1800497 CC genotypes showed higher institutional trust than those with AG/AA + TT/TC (B = 15.67, SE = 5.30, p = 0.02). We could not find any interacting associations among women. While we note that our sample size and candidate gene approach have a potential risk of chance findings, our study provides an important foundation toward further exploration of sex-specific epistatic interaction effects of oxytocin- and dopamine-related genes on trust, indicating the importance of both systems in trust formation.
Content validity of measures in early numeracy in children up to eight years: A COSMIN systematic review
Authors: Speyer Renée , Hakkarainen Airi , Yoon Sangwon , Kim Jae-Hyun , Windsor Catriona , Wilkes Gillan Sarah , Littlefair David , Cordier Reinie
Published: September 2024

Early numeracy skills are considered essential predictors for later mathematical and educational achievement. Therefore, there is a need for early numeracy measures with psychometrically sound properties. This systematic review aimed to determine the content validity of all current early numeracy measures in accordance with the COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN) framework and methodological guidelines, and was conducted and reported by following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 statement and checklist. Systematic literature searches were conducted in January 2024 in five electronic databases: CINAHL, Embase, Eric, PsycINFO, and PubMed. Eligible measures assessed numeracy, targeted children up to eight years of age, were published in English in 1995 or later, and had psychometric data on measure dimensionality. Eligible psychometric reports that were published in English described instrument development and/or content validity of included measures. The measures’ methodological quality was assessed using the COSMIN Risk of Bias checklist, after which all three aspects of content validity (i.e., relevance, comprehensiveness and comprehensibility) were evaluated. Six early numeracy measures and eleven psychometric reports were included. None of the measures could be recommended for use in clinical practice, education, or research due to a lack of high-quality evidence on content validity. However, no high-quality evidence was found to indicate insufficient content validity, therefore, all measures still have the potential to be used. Limited access to measures in the domain of early numeracy, despite having contacted both publishers and instrument developers, may have negatively impacted the completeness of the current overview of content validity of early numeracy measures. In line with the COSMIN guidelines, after the initial evaluation of content validity, future studies should evaluate the remaining psychometric properties of the included measures to identify the most robust measures in terms of validity, reliability, and responsiveness.
PUPAID: A R + ImageJ pipeline for thorough and semi-automated processing and analysis of multi-channel immunofluorescence data
Authors: Régnier Paul , Montardi Camille , Maciejewski-Duval Anna , Marques Cindy , Saadoun David
Published: September 2024

PUPAID is a workflow written in R + ImageJ languages which is dedicated to the semi-automated processing and analysis of multi-channel immunofluorescence data. The workflow is designed to extract fluorescence signals within automatically-segmented cells, defined here as Areas of Interest (AOI), on whole multi-layer slides (or eventually cropped sections of them), defined here as Regions of Interest (ROI), in a simple and understandable yet thorough manner. The included (but facultative) R Shiny-based interactive application makes PUPAID also suitable for scientists who are not fluent with R programming. Furthermore, we show that PUPAID identifies significantly more cells, especially in high-density regions, as compared to already published state-of-the-art methods such as StarDist or Cellpose. For extended possibilities and downstream compatibility, single cell information is exported as FCS files (the standardized file format for single cell-based cytometry data) in order to be openable using any third-party cytometry analysis software or any analysis workflow which takes FCS files as input.
Comorbidities, medications, depression, and physical performance measures associated with severe cognitive impairments in community-dwelling adults
Authors: Almutairi Gamar R. , Almegbas Noura R. , Alosaimi Rawan M. , Alqahtani Maha A. , Batook Saleh G. , Alfageh Ibrahim A. , Alshehri Mohammed M. , Alanazi Shuruq F. , Alhowimel Ahmed S. , Alqahtani Bader A. , Alhwoaimel Norah A. , Alenazi Aqeel M.
Published: September 2024

Cognitive impairment negatively impacts health, psychological, social, and economic domains. Cognitive impairment commonly affects physical functions in older adults, whereas these are deteriorated. However, the prevalence and associated factors of cognitive impairment among community-dwelling adults in Saudi Arabia have not been investigated yet. This study aimed to examine the prevalence of severe cognitive impairment and its associated factors in community-dwelling older adults in Saudi Arabia. This cross-sectional study involved adults aged ≥50 years. Demographic data and clinical data, including number of medications and body mass index (BMI), were collected. Cognitive impairment and depressive symptoms were measured using Arabic versions of the Montreal Cognitive Assessment (MoCA) and Patient Health Questionnaire-9 (PHQ-9), respectively. The participants were divided into severe cognitive impairment and mild cognitive impairment or normal cognitive function groups based on a score of <20 or ≥20, respectively, using the MoCA. Physical measures included dynamic gait index (DGI) scores, timed up-and-go (TUG), 5 times sit-to-stand (5XSST), functional reach test, and 6-minute walk test. A total of 206 participants (female: n = 96) were included. The prevalence of severe cognitive impairment in the community-dwelling older adults was 12.6%. The number of chronic conditions (odds ratio [OR]: 2.31, p<0.001), number of medications (OR: 1.36, p = 0.003), and depressive symptoms using PHQ-9 (OR: 1.11, p = 0.009) were significantly associated with severe cognitive impairment after adjustment for other covariates, including age, sex, and BMI. Based on the physical function measures, only the scores for DGI (OR: 0.86, p = 0.003), TUG (OR: 1.16, p = 0.035) and 5XSST (OR: 1.25, p <0.001) were significantly associated with severe cognitive impairment. This study revealed a high prevalence of severe cognitive impairment among community-dwelling adults in Saudi Arabia. Its major risk factors include depressive symptoms, number of chronic conditions and medications, and physical measures, including DGI, TUG, and 5XSST.
The associations between resilience, self-care, and burnout among medical students
Authors: Michael Keren , Schujovitzky Dana , Karnieli-Miller Orit
Published: September 2024

Burnout is a work-related stress syndrome with substantial consequences for patients, physicians, and medical students. Personal resilience, i.e., the ability to bounce back and thrive despite challenging circumstances, and certain practices, such as self-care, may protect individuals from burnout. However, limited information exists on the complex relationships between resilience, different self-care practices, and burnout. Understanding these associations is important for designing efficient interventions within medical schools. Therefore, the present study examined the direct and indirect associations through a cross-sectional study among 95 fourth-year medical students. Self-reported questionnaires measured resilience, self-care dimensions (stress management, spiritual growth, interpersonal relations, health responsibility), and burnout dimensions (emotional exhaustion, depersonalization, personal accomplishment). Data were analyzed via IBM-SPSS and PROCESS-macro. The main results demonstrated that self-care mediated the associations between resilience and burnout: stress management and interpersonal relations mediated the associations with emotional exhaustion, while spiritual growth mediated the association with personal accomplishment. These results highlight that medical students’ resilience may encourage self-care behaviors, thus decreasing levels of the burnout dimensions of emotional exhaustion and personal accomplishment. Developing curricula that enhance students’ resilience through applying self-care techniques in stressful situations may reduce the negative impact of burnout in healthcare.
Evaluation of knowledge and attitude concerning augmented renal clearance among physicians and clinical pharmacists in Al-Ain, UAE: A cross-sectional study
Authors: Alshouli Betoul , Abbas Maram O. , Alsharji Raniah , Jaber Ammar Ali Saleh
Published: September 2024

Background

Kidney function assessment is crucial in critical illness patients and is required before administering renally excreted medication, especially antibiotics and antiepileptics. Conventional clinical practice often focuses on renal impairment with low creatinine clearance (CrCl) and overlooks the augmented renal clearance (ARC), which is defined by (CrCl) more than 130 ml/min. This typical demonstration neglects individuals who experience hyperfunctioning kidneys. Among critically ill patients, the prevalence of (ARC) is approximately 20% to 65% of cases. This study aims to evaluate physicians’ and clinical pharmacists’ knowledge about ARC-associated risk factors, antibiotic regimen modification in ARC patients, and attitudes towards ARC workshops and guidelines in Al-Ain, UAE.

Methods

A cross-sectional, online self-administered survey-based study was designed to achieve this study’s aim. The questionnaire was constructed on profound literature analysis, validated, and piloted. The survey was emailed to physicians and pharmacists working in two hospitals, private and governmental, and distributed through different social media platforms over three months, December 2022—February 2023.

Results

Of the 92 complete responses (32 clinical pharmacists, 60 physicians), 57 (61.9%), were aware of ARC, but 72 (78%) demonstrated poor knowledge overall. Clinical pharmacists had a higher mean rank of knowledge than the physician’s group. Meanwhile, 70 (76.1%) participants were unaware of the eGFR threshold to determine ARC. There is a noticeable positive attitude toward seeking more information about antibiotic dose adjustment in ARC patients at 85 (92%) of the respondents. Remarkably, only 28 (30.4%) were directly involved with ARC patients’ treatment plans.

Conclusion

In conclusion, clinical pharmacists showed better knowledge than physicians. However, overall, the participating healthcare providers lacked knowledge about ARC, so a reliable source of information regarding ARC should be utilized. Future research could explore the implementation of professional development workshops for healthcare providers and national guidelines and then assess their impact on patient outcomes.
Designing of a chimeric multiepitope vaccine against bancroftian lymphatic filariasis through immunoinformatics approaches
Authors: Parvin Rehana , Habib Ullah Masum Md. , Ferdous Jannatul , Mahdeen Ahmad Abdullah , Shafiqul Islam Khan Md.
Published: September 2024

The filarial worms of Wuchereria bancrofti are the primary cause of lymphatic filariasis (LF), a mosquito-borne disease among the neglected tropical parasitic diseases. Considering the global endemic consequences of the disease, there is a need to develop a successful vaccine candidate against LF. Using advanced immunoinformatics approaches, we designed two multiepitope vaccines targeting W. bancrofti’s glutathione S-transferase and thioredoxin. Therefore, we predicted several MHC-1, MHC-2, and B-cell epitopes from these proteins and mapped two vaccine candidates (V1 and V2). The vaccines were subsequently employed for physicochemical analysis, structural prediction and validation, docking and normal mode analysis, codon optimization, and immune simulation. The selected MHC-1, MHC-2, and B-cell epitopes were antigenic without allergenicity or toxicity. The designed vaccines were expected to be soluble, stable proteins under physiological conditions. Compared to V2, V1’s secondary and tertiary structures were simultaneously favorable, with Ramachandran plot analysis revealing 95.6% residues in favored areas. Subsequently, the molecular docking analysis indicated that the V1 had a high binding affinity for the TLR-2, TLR-4 and TLR-5, as suggested by the docking scores of -1248.7, -1038.5 and -1562.8, respectively. The NMA of these complexes further indicated their structural flexibility. Molecular dynamics simulations of V1-TLR complexes revealed V1-TLR-4 as the most stable, with the lowest free energy and minimal fluctuations, indicating the strongest binding affinity. The results of the codon optimization showed high levels of expression, with a favorable CAI score (<1.0). A three-dose vaccination analysis showed significant and persistent immunological responses, including adaptive and innate immune responses. The findings emphasize the potential of the V1 against W. bancrofti, but further validation is required through in vitro, in vivo, and clinical trials.
Comparison of RNN-LSTM, TFDF and stacking model approach for weather forecasting in Bangladesh using historical data from 1963 to 2022
Authors: Hasan Md. Mahmudul , Hasan Md. Jahid , Rahman Parisha Binte
Published: September 2024

Forecasting the weather in an area characterized by erratic weather patterns and unpredictable climate change is a challenging endeavour. The weather is classified as a non-linear system since it is influenced by various factors that contribute to climate change, such as humidity, average temperature, sea level pressure, and rainfall. A reliable forecasting system is crucial in several industries, including transportation, agriculture, tourism, & development. This study showcases the effectiveness of data mining, meteorological analysis, and machine learning techniques such as RNN-LSTM, TensorFlow Decision Forest (TFDF), and model stacking (including ElasticNet, GradientBoost, KRR, and Lasso) in improving the precision and dependability of weather forecasting. The stacking model strategy entails aggregating multiple base models into a meta-model to address issues of overfitting and underfitting, hence improving the accuracy of the prediction model. To carry out the study, a comprehensive 60-year meteorological record from Bangladesh was gathered, encompassing data on rainfall, humidity, average temperature, and sea level pressure. The results of this study suggest that the stacking average model outperforms the TFDF and RNN-LSTM models in predicting average temperature. The stacking average model achieves an RMSLE of 1.3002, which is a 10.906% improvement compared to the TFDF model. It is worth noting that the TFDF model had previously outperformed the RNN-LSTM model. The performance of the individual stacking model is not as impressive as that of the average model, with the validation results being better in TFDF.
Distribution of MRI-derived T2 values as a biomarker for in vivo rapid screening of phenotype severity in mdx mice
Authors: Waters Emily A. , Haney Chad R. , Vaught Lauren A , McNally Elizabeth M. , Demonbreun Alexis R.
Published: September 2024

Background

The pathology in Duchenne muscular dystrophy (DMD) is characterized by degenerating muscle fibers, inflammation, fibro-fatty infiltrate, and edema, and these pathological processes replace normal healthy muscle tissue. The mdx mouse model is one of the most commonly used preclinical models to study DMD. Mounting evidence has emerged illustrating that muscle disease progression varies considerably in mdx mice, with inter-animal differences as well as intra-muscular differences in pathology in individual mdx mice. This variation is important to consider when conducting assessments of drug efficacy and in longitudinal studies. We developed a magnetic resonance imaging (MRI) segmentation and analysis pipeline to rapidly and non-invasively measure the severity of muscle disease in mdx mice.

Methods

Wildtype and mdx mice were imaged with MRI and T2 maps were obtained axially across the hindlimbs. A neural network was trained to rapidly and semi-automatically segment the muscle tissue, and the distribution of resulting T2 values was analyzed. Interdecile range and Pearson Skew were identified as biomarkers to quickly and accurately estimate muscle disease severity in mice.

Results

The semiautomated segmentation tool reduced image processing time approximately tenfold. Measures of Pearson skew and interdecile range based on that segmentation were repeatable and reflected muscle disease severity in healthy wildtype and diseased mdx mice based on both qualitative observation of images and correlation with Evans blue dye uptake.

Conclusion

Use of this rapid, non-invasive, semi-automated MR image segmentation and analysis pipeline has the potential to transform preclinical studies, allowing for pre-screening of dystrophic mice prior to study enrollment to ensure more uniform muscle disease pathology across treatment groups, improving study outcomes.
Interventions to enhance mental health and wellbeing among international college students: A systematic review and meta-analysis protocol
Authors: Liu Cheng-Ching , Huang Qi , Chen Angela Chia-Chen , Liu Charles , Liu Yuqing
Published: September 2024

Background

International students contribute significantly to both the economy and the intellectual and cultural landscape of host countries. Their interactions with domestic students foster personal, socioeconomic, and political development, promopting a broader understanding of diverse cultures and values. This highlights how crucial international education is for staying competitive globally. However, international students often face challenges such as poor mental health, linguistic and cultural barriers, acculturative stress, and limited health literacy. Therefore, supporting their academic success and well-being on college campuses is essential. This protocol aims to describe strategies used to evaluate the effect of interventions on international students’ mental health and wellbeing and propose directions for future research based on the evidence.

Methods

We will conduct an extensive search in several databases including CINAHL, PubMed, Web of Science, PsyInFO, ERIC, and Google Scholar with no date limits. Two reviewers will independently screen the literature and extract data. We will then conduct meta-analyses of the extracted data.

Discussion

To the best of our knowledge, this study is the first systematic review with meta-analysis focusing on interventions to enhance mental health and wellbeing among international college students. This study will provide most updated empirical evidence on the effects of interventions aimed to improve international students’ mental health and wellbeing. The findings from this study will summarize the importance of a range of interventions being available to international students who experience psychological distress and the effectiveness of each intervention. This study will also highlight the gap for researchers to focus on for future studies.

Trial registration

PROSPERO registration number: CRD42024528767.
Isolation limits spring pollination in a UK fragmented landscape
Authors: Li Dongbo , Clements Christopher F. , Memmott Jane
Published: September 2024

Animal-mediated pollination determines the reproductive success of most flowering plants; this process however can be disrupted by environmental degradation, with habitat loss and fragmentation highlighted as a top driver of pollination deficits. Despite being a pervasive stressor worldwide, we still have rather limited empirical evidence on its effects on pollination services, especially for early spring pollination syndromes. We investigate this using a potted plant phytometry experiment in which we placed English Bluebell (Hyacinthoides non-scripta)—a species largely pollinated in spring—into a fragmented woodland habitat. We selected 51 woodland patches which varied in both size and distance from each other and placed 153 pots of bluebell plants in the patches for c.4 weeks to measure pollination. The woodlands were located in a matrix of grassland, the latter being of low plant species richness and overall in the patches, woodland plants showed a positive species-area relationship. We collected traits on bluebell reproduction, these included the number, size, quality of seeds, the number of seed capsules and the number of flowers that failed to set any seeds. We found that seed traits responded differently to patch area and isolation. Patch isolation negatively affected the number of seeds and capsules, whilst it did not affect the size and quality of seeds. Patch area had no effect on any traits, suggesting that patch area might not necessarily be a factor that affects pollination in this species. The number of flowers that failed to set seed was unaffected by either patch area or isolation. Our study suggests that woodland fragmentation impacts the pollination of understory spring flowering plants. Our results highlight the use of multiple traits of phytometer plants to evaluate pollination and the importance of connectivity in maintaining pollination services in small-fragmented landscapes.
Round gobies (Neogobius melanostomus) in the River Rhine: Population genetic support for invasion via two different routes
Authors: Lampert Kathrin P. , Heermann Lisa , Storm Svenja , Hirsch Philipp Emanuel , Cerwenka Alexander F. , Heubel Katja , Borcherding Jost , Waldvogel Ann-Marie
Published: September 2024

The round goby, Neogobius melanostomus, is a successful invasive fish species. Originating from the Caspian and Black Sea, it is now distributed widely within European fresh- and brackish waters. The River Rhine was colonized in 2008 only a few years after the opening of the Rhine-Main-Danube canal and only four years after N. melanostomus was first reported in the upper Danube River. Its invasion history of the River Rhine was unclear because the species was first detected close to the Rhine river delta which would suggest a route of invasion other than via the Rhine-Main-Danube canal. To investigate the colonization history of N. melanostomus in the Rhine, we combined abundance estimates with molecular analysis. Abundance estimates found N. melanostomus to be dominant in the Lower Rhine (> 90% of all catches). Molecular analysis was done on 286 individuals from four different sites. Using the mitochondrial control region (d-loop), we found three different haplotypes in both Rhine sites. None of the potential invasive source populations in the rivers Danube and Trave exhibited all three haplotypes. The molecular data therefore supported a scenario of two different colonization directions. Our results show that the invasion history of the River Rhine is complex and warrants further investigation.