bluuebunny/crossref_metadata_2025 huggingface dataset:
The vertical structure and variability of currents east of Philippines from mooring measurements during the 2015/2016 El Niño
Authors: Liu Yansong , Ren Qiang , Yu Fei , Wei Chuanjie , Diao Xinyuan , Nan Feng
Published: March 2023

Surface and Subsurface currents in the Philippine Sea have been reported previously, and the debate persists regarding whether they are related to the strength of ENSO (El Niño/Southern Oscillation). We investigated the mean vertical structure and variability of the currents along the western boundary of the Philippine Sea using mooring observations at about 8°N, 127°E from August 2014 to January 2016. The southward Mindanao Current (MC) exists in the upper 600 m countered by the northward Mindanao Undercurrent (MUC) observed below 600 m to the depth of 2,000 m, with a core at approximately 1,000 m. During the development of El Niño from July to December 2015, the core velocity and depth of the MC were noticeably increased, consistent with the Niño 3.4 index, and the current at 2,000 m was observed to be southward, which indicated that the MC may extend to 2,000 m under the influence of El Niño. The MUC was stronger around November 2015, corresponding to the peak phase of the Niño 3.4 index. The intraseasonal variability with a period of 70-110 days from the surface to 2,000 m is also discussed based on power spectral analysis. Subsurface eddies are likely the source of the intraseasonal variability of current in the Philippine Sea from the model outputs.
Characterization and pathogenicity of a novel variant infectious bursal disease virus in China
Authors: Huang Yuanling , Shu Gang , Huang Cong , Han Jingyi , Li Jia , Chen Hongjun , Chen Zongyan
Published: March 2023

Infectious bursal disease (IBD) is a highly epidemic and immunosuppressive disease of 3- to 6-week-old chicks caused by infectious bursal disease virus (IBDV). Since 2017, there has been a notable increase in the isolation rates of novel variant IBDV strains in China, of which characteristic amino acid residues were different from those of early antigen variants. In this study, one IBDV strain was isolated from a farm with suspected IBD outbreak in Shandong Province, China, which was designated LY21/2. The strain LY21/2 could replicate in MC38 cells with previous culture adaption in SPF chick embryos. Phylogenetic analysis revealed that LY21/2 formed one branch with novel variant IBDVs and shared 96.8–98.6% nucleotide sequence identity with them. Moreover, LY21/2 serving as the major parent underwent the recombination event of a variant strain (19D69), while the minor parent was a very virulent strain (Harbin-1). SPF chicks inoculated with LY21/2 showed no gross clinic symptom, whereas bursal atrophy was exhibited and apoptosis was occurred in 55.21% of bursal cells. The results of histopathology and immunohistochemical staining showed that lymphocyte depletion and connective tissue hyperplasia and IBDV antigen-positive cells were observed in the bursa of LY21/2-infected chicks. Besides, DNA fragmentation was detected in the LY21/2-infected bursal tissue section by TUNEL assay. Collectivtely, these data presented analysis and evaluation of the genetic characteristics and pathogenicity of a novel variant IBDV strain. This study may help in the development of biosafety strategies for the prevention and control of IBDV in poultry.
Community assembly and network structure of epiphytic and endophytic phyllosphere fungi in a subtropical mangrove ecosystem
Authors: Zhu Chunchao , Lin Yumiao , Wang Zihui , Luo Wenqi , Zhang Yonghua , Chu Chengjin
Published: March 2023

Microorganisms can influence plant growth and health, ecosystem functioning, and stability. Community and network structures of mangrove phyllosphere fungi have rarely been studied although mangroves have very important ecological and economical values. Here, we used high throughput sequencing of the internal transcribed spacer 2 (ITS2) to assess epiphytic and endophytic phyllosphere fungal communities of six true mangrove species and five mangrove associates. Totally, we obtained 1,391 fungal operational taxonomic units (OTUs), including 596 specific epiphytic fungi, 600 specific endophytic fungi, and 195 shared fungi. The richness and community composition differed significantly for epiphytes and endophytes. Phylogeny of the host plant had a significant constraint on epiphytes but not endophytes. Network analyses showed that plant–epiphyte and plant–endophyte networks exhibited strong specialization and modularity but low connectance and anti-nestedness. Compared to plant–endophyte network, plant–epiphyte network showed stronger specialization, modularity, and robustness but lower connectance and anti-nestedness. These differences in community and network structures of epiphytes and endophytes may be caused by spatial niche partitioning, indicating their underlying ecological and environmental drivers are inconsistent. We highlight the important role of plant phylogeny in the assembly of epiphytic but not endophytic fungal communities in mangrove ecosystems.
Effects of Eimeria acervulina infection on the luminal and mucosal microbiota of the duodenum and jejunum in broiler chickens
Authors: Campos Philip M. , Miska Katarzyna B. , Jenkins Mark C. , Yan Xianghe , Proszkowiec-Weglarz Monika
Published: March 2023

The intestinal disease coccidiosis, caused by Eimeria parasites, impacts nutrient absorption in broiler chickens, leading to weight gain depression and major losses in the poultry industry. To develop alternatives to antibiotics for treating infected chickens, the gut microbiota has been researched because of its association with health factors such as nutrient exchange, immune system modulation, digestive system physiology, and pathogen exclusion. The aim of this study was to determine the effect of Eimeria acervulina infection on the luminal and mucosal microbiota of both the duodenum (DuoL and DuoM) and jejunum (JejL and JejM) at multiple time points (days 3, 5, 7, 10, and 14) post-infection. 16S rRNA amplicon sequencing was utilized to characterize the microbiota and analyze differences in alpha and beta diversity between infected (IF) and control (C) birds at each time point. Alpha diversity differed between IF and C birds in DuoM and JejM microbiota. Combined with beta diversity results, DuoM microbiota appeared to be affected by infection in the longer-term, while JejM microbiota were affected in the shorter-term. Relative abundances of bacterial taxa known for short-chain fatty acid (SCFA) production, such as Lachnospiraceae, Subdoligranulum, and Peptostreptococcaceae, tended to be lower in IF birds for all four microbiota. Moreover, predicted functional abundances showed MetaCyc pathways related to SCFA production, especially butyrate, may be influenced by these differences in bacterial relative abundance. Our findings expand understanding of how Eimeria infection affects luminal and mucosal microbiota in the duodenum and jejunum, and further research on metagenomic function may provide insights on the degree of influence duodenal and jejunal bacteria have on chicken health.
Synergies between synaptic and HCN channel plasticity dictates firing rate homeostasis and mutual information transfer in hippocampal model neuron
Authors: Rathour Rahul Kumar , Kaphzan Hanoch
Published: March 2023

Homeostasis is a precondition for any physiological system of any living organism. Nonetheless, models of learning and memory that are based on processes of synaptic plasticity are unstable by nature according to Hebbian rules, and it is not fully clear how homeostasis is maintained during these processes. This is where theoretical and computational frameworks can help in gaining a deeper understanding of the various cellular processes that enable homeostasis in the face of plasticity. A previous simplistic single compartmental model with a single synapse showed that maintaining input/output response homeostasis and stable synaptic learning could be enabled by introducing a linear relationship between synaptic plasticity and HCN conductance plasticity. In this study, we aimed to examine whether this approach could be extended to a more morphologically realistic model that entails multiple synapses and gradients of various VGICs. In doing so, we found that a linear relationship between synaptic plasticity and HCN conductance plasticity was able to maintain input/output response homeostasis in our morphologically realistic model, where the slope of the linear relationship was dependent on baseline HCN conductance and synaptic permeability values. An increase in either baseline HCN conductance or synaptic permeability value led to a decrease in the slope of the linear relationship. We further show that in striking contrast to the single compartment model, here linear relationship was insufficient in maintaining stable synaptic learning despite maintaining input/output response homeostasis. Additionally, we showed that homeostasis of input/output response profiles was at the expense of decreasing the mutual information transfer due to the increase in noise entropy, which could not be fully rescued by optimizing the linear relationship between synaptic and HCN conductance plasticity. Finally, we generated a place cell model based on theta oscillations and show that synaptic plasticity disrupts place cell activity. Whereas synaptic plasticity accompanied by HCN conductance plasticity through linear relationship maintains the stability of place cell activity. Our study establishes potential differences between a single compartmental model and a morphologically realistic model.
Microvascular cerebral blood flow response to intrathecal nicardipine is associated with delayed cerebral ischemia
Authors: Sathialingam Eashani , Cowdrick Kyle R. , Liew Amanda Y. , Fang Zhou , Lee Seung Yup , McCracken Courtney E. , Akbik Feras , Samuels Owen B. , Kandiah Prem , Sadan Ofer , Buckley Erin M.
Published: March 2023

One of the common complications of non-traumatic subarachnoid hemorrhage (SAH) is delayed cerebral ischemia (DCI). Intrathecal (IT) administration of nicardipine, a calcium channel blocker (CCB), upon detection of large-artery cerebral vasospasm holds promise as a treatment that reduces the incidence of DCI. In this observational study, we prospectively employed a non-invasive optical modality called diffuse correlation spectroscopy (DCS) to quantify the acute microvascular cerebral blood flow (CBF) response to IT nicardipine (up to 90 min) in 20 patients with medium-high grade non-traumatic SAH. On average, CBF increased significantly with time post-administration. However, the CBF response was heterogeneous across subjects. A latent class mixture model was able to classify 19 out of 20 patients into two distinct classes of CBF response: patients in Class 1 (n = 6) showed no significant change in CBF, while patients in Class 2 (n = 13) showed a pronounced increase in CBF in response to nicardipine. The incidence of DCI was 5 out of 6 in Class 1 and 1 out of 13 in Class 2 (p < 0.001). These results suggest that the acute (<90 min) DCS-measured CBF response to IT nicardipine is associated with intermediate-term (up to 3 weeks) development of DCI.
Severe neurometabolic phenotype in npc1−/− zebrafish with a C-terminal mutation
Authors: Quelle-Regaldie Ana , Gandoy-Fieiras Nerea , Rodríguez-Villamayor Paula , Maceiras Sandra , Losada Ana Paula , Folgueira Mónica , Cabezas-Sáinz Pablo , Barreiro-Iglesias Antón , Villar-López María , Quiroga-Berdeal María Isabel , Sánchez Laura , Sobrido María Jesús
Published: March 2023

Niemann Pick disease type C (NPC) is an autosomal recessive neurodegenerative lysosomal disorder characterized by an accumulation of lipids in different organs. Clinical manifestations can start at any age and include hepatosplenomegaly, intellectual impairment, and cerebellar ataxia. NPC1 is the most common causal gene, with over 460 different mutations with heterogeneous pathological consequences. We generated a zebrafish NPC1 model by CRISPR/Cas9 carrying a homozygous mutation in exon 22, which encodes the end of the cysteine-rich luminal loop of the protein. This is the first zebrafish model with a mutation in this gene region, which is frequently involved in the human disease. We observed a high lethality in npc1 mutants, with all larvae dying before reaching the adult stage. Npc1 mutant larvae were smaller than wild type (wt) and their motor function was impaired. We observed vacuolar aggregations positive to cholesterol and sphingomyelin staining in the liver, intestine, renal tubules and cerebral gray matter of mutant larvae. RNAseq comparison between npc1 mutants and controls showed 284 differentially expressed genes, including genes with functions in neurodevelopment, lipid exchange and metabolism, muscle contraction, cytoskeleton, angiogenesis, and hematopoiesis. Lipidomic analysis revealed significant reduction of cholesteryl esters and increase of sphingomyelin in the mutants. Compared to previously available zebrafish models, our model seems to recapitulate better the early onset forms of the NPC disease. Thus, this new model of NPC will allow future research in the cellular and molecular causes/consequences of the disease and on the search for new treatments.
WSP from “Nostoc commune” Vauch. suppresses gastric cancer migration via EGFRVIII signaling
Authors: Chen Xiaoxia , Bai Wenqi , Liu Xiangrong , Zhao Jiao , Li Zhiyuan , Li Jianrong , Su Liping , Guan Tao , Sun Ruifang , Yang Xihua , Lv Caixia , Wang Zhixiang , Hu Linjie , Li Zheng , Ma Jinfeng , Zhang Huanhu , Lu Xiaoqing
Published: March 2023

Introduction

A number of evidences have proved that “Nostoc commune” Vauch can improve human immunity and prevent diseases, however, the specific mechanism remains unclear. The biological activity of the main protein component of “Nostoc commune” Vauch extracellular matrix– a water-stress protein (WSP) still needs to be elucidated.

Methods

In our study, we validated the role of WSP in gastric cancer metastasis at the cellular level, the organoid level and in mouse models, and also studied the role of EGFRVIII and downstream signaling molecules after WSP treatment.

Results

We found that WSP can significantly inhibit the metastasis of gastric cancer cells. Interestingly, we found that the anti-metastasis ability of WSP on gastric cancer was related to membrane protein receptor EGFRVIII, which was realized by inhibiting the downstream EGFRVIII signaling pathway. In terms of mechanism, WSP can inhibit the downstream EGFRVIII signaling pathway Akt-PI3K and further inhibit the secretion of cancer-related metastasis proteins such as MMP2 and MMP9, thus, significantly affecting the metastasis of gastric cancer cells.

Discussion

Given the anticancer properties of WSP, drug developers and manufacturers can further develop protein drugs for cancer patients using protein engineering techniques based on the properties of WSP.
Association of smoking and ALK tyrosine-kinase inhibitors on overall survival in treatment-naïve ALK-positive advanced lung adenocarcinoma
Authors: Zheng Zhe-Rong , Ku Hsiu-Ying , Chen Kun-Chieh , Chiang Chun-Ju , Wang Chih-Liang , Chen Chih-Yi , Tsai Chun-Ming , Huang Ming-Shyan , Yu Chong-Jen , Chen Jin-Shing , Chou Teh-Ying , Lee Wen-Chung , Wang Chun-Chieh , Liu Tsang-Wu , Hsia Jiun-Yi , Chang Gee-Chen
Published: March 2023

Introduction

Anaplastic lymphoma kinase (ALK) fusion mutation is more common in younger and never-smoking lung cancer patients. The association of smoking and ALK-tyrosine kinase inhibitors (TKIs) on overall survival (OS) of treatment-naïve ALK-positive advanced lung adenocarcinoma remains unclear in real-world.

Methods

This retrospective study evaluated all 33170 lung adenocarcinoma patients registered in the National Taiwan Cancer Registry from 2017 to 2019, of whom 9575 advanced stage patients had ALK mutation data.

Results

Among the 9575 patients, 650 (6.8%) patients had ALK mutation with the median follow-up survival time 30.97 months (median age, 62 years; 125 [19.2%] were aged ≥75 years; 357 (54.9%) females; 179 (27.5) smokers, 461 (70.9%) never-smokers, 10 (1.5%) with unknown smoking status; and 544 (83.7%) with first-line ALK-TKI treatment). Overall, of 535 patients with known smoking status who received first-line ALK-TKI treatment, never-smokers and smokers had a median OS of 40.7 months (95% confidence interval (CI), 33.1-47.2 months) and 23.5 months (95% CI, 11.5-35.5 months) (P=0.015), respectively. Among never-smokers, those who received first-line ALK-TKI treatment had a median OS of 40.7 months (95% CI, 22.7-57.8 months), while those ALK-TKI not as first-line treatment had a median OS of 31.7 months (95% CI, 15.2-42.8 months) (P=0.23). In smokers, the median OS for these patients was 23.5 months (95% CI, 11.5-35.5 months) and 15.6 months (95% CI, 10.2-21.1 months) (P=0.026), respectively.

Conclusions and relevance

For patients with treatment-naïve advanced lung adenocarcinoma, the ALK test should be performed irrespective of smoking status and age. Smokers had shorter median OS than never-smokers among treatment-naïve-ALK-positive patients with first-line ALK-TKI treatment. Furthermore, smokers not receiving first-line ALK-TKI treatment had inferior OS. Further investigations for the first-line treatment of ALK-positive smoking advanced lung adenocarcinoma patients are needed.
The potential of ferroptosis combined with radiotherapy in cancer treatment
Authors: Lu Zekun , Xiao Bingkai , Chen Weibo , Tang Tianyu , Zhuo Qifeng , Chen Xuemin
Published: March 2023

Ferroptosis is a new form of regulatory cell death that is closely related to the balance of redox reactions and the occurrence and development of cancer. There is increasing evidence that inducing ferroptosis in cells has great potential in the treatment of cancer. Especially when combined with traditional therapy, it can improve the sensitivity of cancer cells to traditional therapy and overcome the drug resistance of cancer cells. This paper reviews the signaling pathways regulating ferroptosis and the great potential of ferroptosis and radiotherapy (RT) in cancer treatment and emphasizes the unique therapeutic effects of ferroptosis combined with RT on cancer cells, such as synergy, sensitization and reversal of drug resistance, providing a new direction for cancer treatment. Finally, the challenges and research directions for this joint strategy are discussed.
Clinical significance of enlarged cardiophrenic lymph nodes by CT in advanced ovarian cancer
Authors: Song Sisi , Chen Huizhu , Ning Gang , Guo Yingkun , Li Xuesheng
Published: March 2023

Aim

This study aims to assess the clinical influence of enlarged cardiophrenic lymph nodes (CPLN) on staging computed tomography (CT) among patients with advanced ovarian cancer.

Methods

This retrospective cohort study included 320 patients with advanced epithelial ovarian cancer who underwent staging CT from May 2008 to January 2019. The CPLN diameter was the average of two radiologists’ measurements. Enlarged CPLN was defined as a short-axis diameter of ≥5 mm. Clinical and imaging findings, management decisions, and progression-free survival(PFS) were compared between patients with and without enlarged CPLN.

Results

Enlarged CPLN was found in 129 (40.3%) patients, which was significantly associated with more pelvic peritoneal carcinomatosis (odds ratio [OR]: 6.61 with 95% confidence interval [CI]: 1.51–28.99), and involved the greater omentum (OR: 6.41, 95% CI: 3.05–13.46), spleen capsule nodules (OR: 2.83, 95% CI: 1.58–5.06), and liver capsule nodules (OR: 2.55, 95% CI: 1.57–4.17). The optimal cytoreduction rates did not differ between patients with and without enlarged CPLN (p = 0.656). The presence of enlarged CPLN had a significant negative influence on PFS (median PFS, 23.5 vs. 80.6 months, respectively, CPLN ≥5 mm versus <5 mm; p = 0.023) in patients with no RD after primary debulking surgery, but no adverse effect on PFS among patients with RD (median PFS, 28.0 vs. 24.4 months, respectively, CPLN ≥5 mm versus <5 mm; p = 0.359). However, enlarged CPLN on staging CT did not affect PFS in patients treated with neoadjuvant chemotherapy, with (median PFS, 22.4 vs. 23.6 months, respectively, CPLN ≥5 mm versus <5 mm; p = 0.360) or without RD (median PFS, 17.7 vs. 23.3 months, respectively, CPLN ≥5 mm versus <5 mm; p = 0.400). The enlarged CPLN showed a decreased trend in 81.6% (n = 80) of the patients with enlarged CPLN. No significant difference was found in PFS (p = 0.562) between patients with decreased and increased in the size of CPLN.

Conclusions

Enlarged CPLN on staging CT is associated with more abdominal disease but is not reliable in predicting complete resection. Enlarged CPLN awareness is necessary for patients with a primary chance of complete resection of abdominal disease.
Immunoglobulin a vasculitis with testicular/epididymal involvement in children: A retrospective study of a ten-year period
Authors: Hu Jian-Jun , Zhao Yao-Wang , Wen Rong , Luo Yang-Yang , Zhou Wei-Guo , Liu Yu-Hang , Qin Feng , Liu Chang , He Tian-Qu
Published: March 2023

The clinical characteristics and risk factors for testicular/epididymal involvement in 73 children with immunoglobulin A vasculitis (IgAV) who were admitted to our hospital between January 2012 and November 2022 were reviewed. The demographic data, laboratory parameters, and follow-up data of the patients were compared to those of 146 males without testicular/epididymal involvement. A logistic regression analysis was performed to determine the variables associated with testicular/epididymal involvement. The prevalence of testicular/epididymal involvement among male patients with IgAV was 1.3% (73/5,556). Increased blood flow in the testes and/or epididymis on ultrasound was found in 71 patients. The remaining two patients underwent surgical exploration for loss or reduction of testicular blood flow. One patient underwent orchiectomy for intraoperative confirmation of complete right testicular infarction. Pathological findings revealed IgA immune complex deposition in the testis. Patient age (odds ratio [OR] = 0.792; 95% confidence interval [CI]: 0.682–0.919, p = 0.002), platelet count (OR = 1.011; 95% CI: 1.002–1.020, p = 0.013), and immunoglobulin M (IgM) levels (OR = 0.236; 95% CI: 0.091–0.608, p = 0.003) were strongly associated with the occurrence of testicular/epididymal involvement in IgAV. Therefore, young age, increased platelet count, and low IgM levels in patients with IgAV are potential risk factors for testicular/epididymal involvement. Doppler ultrasound can help differentiate IgAV from acute scrotum. Most patients with testicular/epididymal involvement have good prognoses, although serious complications such as testicular infarction may occur.
Menthol: An underestimated anticancer agent
Authors: Zhao Yijia , Pan Huafeng , Liu Wei , Liu E. , Pang Yaobin , Gao Hongjin , He Qingying , Liao Wenhao , Yao Yejing , Zeng Jinhao , Guo Jing
Published: March 2023

Menthol, a widely used natural, active compound, has recently been shown to have anticancer activity. Moreover, it has been found to have a promising future in the treatment of various solid tumors. Therefore, using literature from PubMed, EMBASE, Web of Science, Ovid, ScienceDirect, and China National Knowledge Infrastructure databases, the present study reviewed the anticancer activity of menthol and the underlying mechanism. Menthol has a good safety profile and exerts its anticancer activity via multiple pathways and targets. As a result, it has gained popularity for significantly inhibiting different types of cancer cells by various mechanisms such as induction of apoptosis, cell cycle arrest, disruption of tubulin polymerization, and inhibition of tumor angiogenesis. Owing to the excellent anticancer activity menthol has demonstrated, further research is warranted for developing it as a novel anticancer agent. However, there are limitations and gaps in the current research on menthol, and its antitumor mechanism has not been completely elucidated. It is expected that more basic experimental and clinical studies focusing on menthol and its derivatives will eventually help in its clinical application as a novel anticancer agent.
Prevalence and associated factors of medication adherence among infertile women undergoing frozen-thawed embryo transfer cycle: A cross-sectional study
Authors: Ni Ying , Tong Chenye , Xu Lianying , Qian Wen , Huang Limin , Zhang Aijun , Fang Qiong
Published: March 2023

Objective: This study aimed to explore the prevalence and associated factors of medication adherence among infertile women undergoing frozen-thawed embryo transfer (FET) cycle.

Methods: A cross-sectional study was conducted with 556 infertile women undergoing FET cycle in total. The Self-efficacy for Appropriate Medication Use Scale (SEAMS), Herth Hope Index (HHI) scale, and Social Support Rating Scale (SSRS) were used to evaluate the patients. Data were described by univariate and multivariate analyses. Logistic regression method was performed to analyse the factors potentially associated with medication adherence.

Results: The average score of Self-efficacy for Appropriate Medication Use Scale (SEAMS) was 30.38 ± 6.65, and 65.3% of participants showed non-adherence. Multiple regression analysis indicated that first-time FET cycle, treatment stage, methods of daily medication, social support and hope level were the main associated factors of the medication adherence among infertile women undergoing FET cycle (p < 0.001).

Conclusion: This study revealed the medication adherence is at medium level among infertile women undergoing FET cycle, especially in patients with repeated FET cycles. The study also suggested that improving the hope level and social support of infertile women undergoing FET cycle may increase medication adherence.
Small deviation properties concerning arrays of non-homogeneous Markov information sources
Authors: Qin Ximei , Rui Zhaobiao , Chen Shu , Peng Weicai
Published: March 2023

In this study, we first define the logarithmic likelihood ratio as a measure between arbitrary generalized information sources and non-homogeneous Markov sources and then establish a class of generalized information sources for small deviation theorems, strong limit theorems, and the asymptotic equipartition property. The present outcomes generalize some existing results.
Evaluation of carbon balance and carbohydrate reserves from forced (Vitis vinifera L.) cv. Tempranillo vines
Authors: Oliver-Manera Jordi , Anić Marina , García-Tejera Omar , Girona Joan
Published: March 2023

Elevated temperatures during berry ripening have been shown to affect grape quality. The crop forcing technique (summer pruning that ‘force’ the vine to start a new cycle) has been shown to improve berry quality by delaying the harvest date. However, yield is typically reduced on forced vines, which is attributed to vine low carbon availability soon after forcing and likely incomplete inflorescence formation. The present study aims to estimate the carbon balance of forced vines and evaluate vine responses to changes in carbon patterns due to forcing. Three treatments were studied on Tempranillo cultivar: non-forced vines (Control), vines forced shortly after fruit set (CFearly) and vines forced one month later at the beginning of bunch closure (CFlate). Whole canopy net carbon exchange was modelled and validated using two whole canopy gas exchange chambers. In addition, non-structural carbohydrate reserves at budburst, forcing date and harvest, were analysed. Yield, yield components and vegetative growth were also evaluated. Harvest date was delayed by one and two months in the CFearly and CFlate, respectively, which increased must acidity. However, yield was lower in the forced treatments compared to the Control (49% lower for CFearly and 82% for CFlate). In the second year, at the time when CFearly and CFlate dormant buds were unlocked (forced budburst), forced vines had significantly lower non-structural carbohydrates than Control vines at budburst. Although the time elapsed from budburst to reach maximum net carbon exchange was longer for the Control treatment (80 days) than for the forced treatments (about 40 days), average daily net carbon exchange until harvest was comparable between Control (60.9 g CO2/vine/day) and CFearly (55.9 g CO2/vine/day), but not for CFlate (38.7 g CO2/vine/day). In addition, the time elapsed from budburst to harvest was shorter in forced treatments (about 124 days) than for the Control (172 days). As a result, the cumulative net carbon exchange until harvest was reduced by 35% (CFearly) and 55% (CFlate) in the forced treatments. However, no differences in carbon reserves at harvest were observed between treatments partly helped by the higher source:sink ratio observed in forced than Control vines.
How rice adapts to high temperatures
Authors: Ren Huimin , Bao Jingpei , Gao Zhenxian , Sun Daye , Zheng Shuzhi , Bai Jiaoteng
Published: March 2023

High-temperature stress affects crop yields worldwide. Identifying thermotolerant crop varieties and understanding the basis for this thermotolerance would have important implications for agriculture, especially in the face of climate change. Rice (Oryza sativa) varieties have evolved protective strategies to acclimate to high temperature, with different thermotolerance levels. In this review, we examine the morphological and molecular effects of heat on rice in different growth stages and plant organs, including roots, stems, leaves and flowers. We also explore the molecular and morphological differences among thermotolerant rice lines. In addition, some strategies are proposed to screen new rice varieties for thermotolerance, which will contribute to the improvement of rice for agricultural production in the future.
Motivation in second language acquisition: A bibliometric analysis between 2000 and 2021
Authors: Wu Xue
Published: March 2023

As one of the most important constructs of individual differences in second language learning, motivation has garnered a lot of attention in the area of Second Language Acquisition (SLA). Nevertheless, little bibliometric research has been conducted to provide a systematic overview of this line of research, which will help researchers to better understand how motivation-related research in SLA has evolved over the past 22 years and where it might push the boundaries of SLA research into in the future. In this study, three types of bibliometric analyses (i.e., co-citation analysis, citation analysis, and keyword analysis) were performed to identify the prominent scholarly documents, authors, venues of publications, and research topics that have been highly influential in the research of motivation in SLA between 2000 and 2021. Results from scientific network maps and keyword analysis suggest significant changes in the topic over the past 22 years. The results in this study also indicate an evident continuity of theoretic development in L2 language learning motivation research. Moreover, an air of active use of qualitative approaches has been detected in L2 language learning motivation research in the last 22 years.
Reflections on the battle against COVID-19: The effects of emotional design factors on the communication of audio-visual art
Authors: Fang Wen-Ting , Sun Jian-Hua , Liang Qing-Dong
Published: March 2023

Fighting against the epidemic is an arduous and prolonged battle where many artists hope to inspire people with the power of art through cultural creativity. To explore the effects of emotional design factors on the communication of audio-visual art and the audience’s perceptive experience, this research takes the original anti-epidemic song and the film China Braves Headwind as the research object. The research also uses such methods as questionnaires, Structural Equation Models, and dependent samples t-tests to conduct statistical analysis. The results are as follows: First, the emotional design evaluation matrix based on the emotional communication model is reasonable, and the scales of this research are feasible. Second, the emotional design of audio-visual works can significantly affect the audience’s emotional experience and further improve sharing intention. Third, Attribute A2 (Artistic style, Thematic perception) and attribute C3 (Emotional resonance, Spiritual sublimation) serve as common factors affecting the emotional experience in terms of both musical works and film and television works. Fourth, compared with musical works, film and television works are likely to resonate with the audience. The combination of music and visual sensation can help open up the conception of artistic works and convey their meanings to viewers. Therefore, it’s necessary to explore the emotional communication mode between audio-visual artists and the audience. It helps artists think about how to create works innovatively and is conducive to marketizing works and stimulating cultural consumption demand.
English-medium instruction in Tunisia: Perspectives of students
Authors: Abdeljaoued Marii
Published: March 2023

This article gives a Tunisian perspective to the ongoing debate on the adoption of English-medium instruction (EMI) across the globe and notably in the Middle East and North Africa (MENA). It explores the attitudes of students toward EMI, especially in relation to French, the default medium of instruction at Tunisian universities. It also investigates the challenges that students encounter in courses mediated through English. Finally, it reports on the current EMI practices as they are conducted in the classroom setting. The article uses a mixed approach involving quantitative data collected via an online survey and qualitative data via classroom observation and note-taking. It was found that students typically held a positive attitude toward English and an awareness of its importance. They displayed a pragmatic stance as they associated English with research, technology, mobility, employability, and career prospects. While English is used as the language of the curriculum and documentation, students engage in translanguaging practices to ensure an effective dialog with content teachers and better acquisition of academic content. Given their multilingual repertoire and the status of French, students used French and English in parallel and, to a lesser extent, Tunisian Arabic. They tended to switch to French to ensure a more effective classroom exchange, especially when English fails them. Teachers used translanguaging in order to promote students’ engagement in the academic content.
The role of paternal and maternal warmth and hostility on daughter’s psychosocial outcomes: The insidious effects of father warmth combined with high paternal hostility
Authors: Dmitrieva Julia , Espel Emma V.
Published: March 2023

Introduction

Despite the well-accepted view on the importance of parental warmth and parental hostility for adolescent development, few studies have examined the joint interactive effects of these two key aspects of parenting. Furthermore, research comparing maternal and paternal parenting is limited, with the father-daughter relationship during adolescence remaining one of the more understudied familial contexts. Given that family processes are key for the intergenerational transmission of inequality, these parent–child relationships may be especially important for youth at risk for exposure to violence.

Objectives

Using a sample of juvenile female offenders, this study examined the associations between the perceived warmth and hostility in the father-daughter and mother-daughter relationships on daughters’ depressive symptoms, anxiety symptoms, romantic partner warmth, romantic partner hostility, and the daughter’s sense of agency. We hypothesized that high perceived parental warmth would moderate the effects of parental hostility by protecting daughters from the negative effects of parental hostility, with stronger effects for the father-daughter than the mother-daughter relationship.

Results

In contrast, our paternal relationship findings across four of the five outcomes suggest a moderation in the opposite direction – that is, high perceived father warmth exacerbates the deleterious effects of father hostility on daughters’ depressive symptoms, anxiety, romantic partner warmth, and romantic partner hostility. Maternal warmth, and not hostility, had a direct association with these four outcomes, with stronger explanatory power shown for the father-daughter than the mother-daughter model. Higher agency was associated with maternal hostility only.

Conclusion

Our findings suggest that daughters might be modeling and internalizing the relationship with their fathers (for better or worse) when they perceive it as warm and supportive. Consequently, adolescent girls whose fathers exhibit hostile behavior may benefit from emotional distancing from their fathers.
Dynamic characteristics of a COVID-19 outbreak in Nanjing, Jiangsu province, China
Authors: Wang Junjun , Ma Tao , Ding Songning , Xu Ke , Zhang Min , Zhang Zhong , Dai Qigang , Tao Shilong , Wang Hengxue , Cheng Xiaoqing , He Min , Du Xuefei , Feng Zhi , Yang Huafeng , Wang Rong , Xie Chaoyong , Xu Yuanyuan , Liu Li , Chen Xupeng , Li Chen , Wu Wen , Ye Sheng , Yang Sheng , Fan Huafeng , Zhou Nan , Ding Jie
Published: March 2023

Objectives

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lineage B.1.617.2 (also named the Delta variant) was declared as a variant of concern by the World Health Organization (WHO). This study aimed to describe the outbreak that occurred in Nanjing city triggered by the Delta variant through the epidemiological parameters and to understand the evolving epidemiology of the Delta variant.

Methods

We collected the data of all COVID-19 cases during the outbreak from 20 July 2021 to 24 August 2021 and estimated the distribution of serial interval, basic and time-dependent reproduction numbers (R0 and Rt), and household secondary attack rate (SAR). We also analyzed the cycle threshold (Ct) values of infections.

Results

A total of 235 cases have been confirmed. The mean value of serial interval was estimated to be 4.79 days with the Weibull distribution. The R0 was 3.73 [95% confidence interval (CI), 2.66–5.15] as estimated by the exponential growth (EG) method. The Rt decreased from 4.36 on 20 July 2021 to below 1 on 1 August 2021 as estimated by the Bayesian approach. We estimated the household SAR as 27.35% (95% CI, 22.04–33.39%), and the median Ct value of open reading frame 1ab (ORF1ab) genes and nucleocapsid protein (N) genes as 25.25 [interquartile range (IQR), 20.53–29.50] and 23.85 (IQR, 18.70–28.70), respectively.

Conclusions

The Delta variant is more aggressive and transmissible than the original virus types, so continuous non-pharmaceutical interventions are still needed.
Occupational health risk assessment of workplace solvents and noise in the electronics industry using three comprehensive risk assessment models
Authors: Huang Qifan , Su Shibiao , Zhang Xiaoshun , Li Xiang , Zhu Jiawei , Wang Tianjian , Wen Cuiju
Published: March 2023

Background

Occupational hazards such as solvents and noise in the electronics industry are serious. Although various occupational health risk assessment models have been applied in the electronics industry, they have only been used to assess the risks of individual job positions. Few existing studies have focused on the total risk level of critical risk factors in enterprises.

Methods

Ten electronics enterprises were selected for this study. Information, air samples and physical factor measurements were collected from the selected enterprises through on-site investigation, and then the data were collated and samples were tested according to the requirements of Chinese standards. The Occupational Health Risk Classification and Assessment Model (referred to as the Classification Model), the Occupational Health Risk Grading and Assessment Model (referred to as the Grading Model), and the Occupational Disease Hazard Evaluation Model were used to assess the risks of the enterprises. The correlations and differences between the three models were analyzed, and the results of the models were validated by the average risk level of all of the hazard factors.

Results

Hazards with concentrations exceeding the Chinese occupational exposure limits (OELs) were methylene chloride, 1,2-dichloroethane, and noise. The exposure time of workers ranged from 1 to 11 h per day and the frequency of exposure ranged from 5 to 6 times per week. The risk ratios (RRs) of the Classification Model, the Grading Model and the Occupational Disease Hazard Evaluation Model were 0.70 ± 0.10, 0.34 ± 0.13, and 0.65 ± 0.21, respectively. The RRs for the three risk assessment models were statistically different (P < 0.001), and there were no correlations between them (P > 0.05). The average risk level of all of the hazard factors was 0.38 ± 0.18, which did not differ from the RRs of the Grading Model (P > 0.05).

Conclusions

The hazards of organic solvents and noise in the electronics industry are not negligible. The Grading Model offers a good reflection of the actual risk level of the electronics industry and has strong practicability.
Neuro-imaging characteristics of sensory impairment in cerebral palsy; a systematic review
Authors: Knijnenburg A. C. S. , Steinbusch C. V. M. , Janssen-Potten Y. J. M. , Defesche A. , Vermeulen R. J.
Published: March 2023

Background

Objective: To identify and examine neural reorganization of the sensory network in terms of lesion type, somatotopic organization of the primary somatosensory area, and functional connectivity in relation to sensory function in children and young adults with cerebral palsy (CP).

Methods

Design: systematic review, Prospero registration ID 342570. Data sources: PubMed; Cochrane; Web of Science; Embase; CINAHL and PEDro from inception to March 13, 2021. Eligibility criteria: All types of original studies, concerning sensory connectivity in relation to sensory outcome in patients with spastic CP, <30 years of age. No publication status or date restrictions were applied. Data extraction and synthesis: Two authors independently determined the eligibility of studies. Quality assessment was performed by a third author. Neuro-imaging/neurophysiological techniques, sensory outcomes and patient characteristics were extracted.

Results

Children and young adults with periventricular leucomalacia (PVL) lesions have significantly better hand function and sensation scores than patients with cortical-subcortical/middle cerebral artery (MCA) lesions. Ipsilesional reorganization of the S1 (primary somatosensory cortex) area appears to be the primary compensation mechanism after a unilateral early brain lesion, regardless of the timing of the lesion. Interhemispheric reorganization of the sensory system after early brain lesions is rare and, when it occurs, poorly effective. Diffusion tractography shows a positive correlation between the ascending sensory tract (AST) diffusivity metrics of the more affected hemisphere and sensory test outcomes.

Discussion and conclusions

Because of the large variability in study design, patient characteristics, neuroimaging/neurophysiological techniques and parameters as well as sensory assessment methods used, it is difficult to draw definite inferences on the relationship between the reorganization of the sensory network following early brain damage and sensory function in children and young adults with CP. In general, sensory function seems to be worse in cortical as opposed to white matter tract (PVL) lesions. International consensus on a clinically relevant sensory test battery is needed to enhance understanding of the intriguing compensatory mechanisms of sensory network following early brain damage and potential consequences for rehabilitation strategies.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/.
The effect of acids precipitants on the synthesis of WO3 hierarchical nanostructures for highly selective and sensitive H2S detection
Authors: Mpanza Thokozani , Ogundipe Sunday A. , Ndlangamandla Ceboliyozakha L. , Swart Hendrik C. , Nkosi Steven S.
Published: March 2023

The detection and monitoring of H2S gas at high and lower concentrations is very crucial since this gas is highly toxic and can affect tissues and organs, especially in occupational environment. This work reports on the synthesis of WO3 nanostructures-based sensors for highly sensitive and selective H2S detection at low operating temperatures. These WO3 nanostructures were synthesized using pressurized hydrothermal process. Different acids from weak to strong (HNO3, H2SO4, and HCl) were employed as precipitants to form supposedly hierarchical and cube-like nanostructures of WO3. These WO3 nanostructures were characterized by XRD, SEM, TEM, XPS and BET analysis. The fabricated WO3 sensors were exposed to different target gases (CO2, H2, CH4, NH3, LPG and H2S) at different concentrations. They were found to be selective to H2S, and the WO3 precipitated by HCl otherwise referred to as WO3-HCl was found to be highly sensitive, with high response of S = 1394.04 towards 150 ppm of H2S at 125°C operating temperature. The WO3 precipitated by H2SO4 named WO3-H2SO4 showed a high response of 141.64 at 125°C operating temperature. Lastly, WO3 precipitated by HNO3 called WO3-HNO3, recorded a H2S response of 125.75 also at 125°C operating temperature. The HCl-precipitated WO3 is a promising candidate for selective detection of H2S, being the most sensitive in the series.
The predictive power of magnetospheric models for estimating ground magnetic field variation in the United Kingdom
Authors: Florczak Ewelina , Beggan Ciarán D. , Whaler Kathryn A.
Published: March 2023

Space weather events can have damaging effects on ground-based infrastructure. Geomagnetically induced currents (GIC) caused by rapid magnetic field fluctuations during geomagnetic storms can negatively affect power networks, railways as well as navigation systems. To reduce such negative impacts, good forecasting capability is essential. In this study we assess the performance of contemporary magnetohydrodynamic (MHD) models in predicting the external-only ground magnetic field perturbations at three United Kingdom observatories during two severe space weather events: September 2017 and March 2015. Simulated magnetic data were acquired via Community Coordinated Modeling Center (CCMC), using the following models: Space Weather Modeling Framework (SWMF), Open Geospace General Circulation Model (Open GGCM) and Lyon–Fedder–Mobarry (LFM) combined with the Rice Convection Model (RCM). All simulations use spacecraft measurements at L1 as their solar wind input in calculating ground perturbations. Qualitative and quantitative comparison between measured and modelled values suggest that the performance of MHD models vary with latitude, the magnetic component and the characteristics of the storm analysed. Most models tend to exaggerate the magnitude of disturbances at lower latitudes but better capture the fluctuations at the highest latitude. For the two storms investigated, the addition of RCM tends to result in overestimation of the amplitude of ground perturbations. The observed data-model discrepancies most likely arise due to the many approximations required in MHD modelling, such as simplified solar wind input or shift in location of the electrojets in the simulated magnetospheric and ionospheric currents. It was found that no model performs consistently better than any other, implying that each simulation forecasts different aspects of ground perturbations with varying level of accuracy. Ultimately, the decision of which model is most suitable depends on specific needs of the potential end user.