bluuebunny/crossref_metadata_2025 huggingface dataset:
Quality of Life, Sexuality, Anatomical Results and Side-effects of Implantation of an Alloplastic Mesh for Cystocele Correction at Follow-up after 36 Months
Authors: Fünfgeld Christian , Stehle Margit , Henne Brigit , Kaufhold Jan , Watermann Dirk , Grebe Markus , Mengel Mathias
Published: September 2017

Introduction Pelvic organ prolapse can significantly reduce quality of life of affected women, with many cases requiring corrective surgery. The rate of recurrence is relatively high after conventional prolapse surgery. In recent years, alloplastic meshes have increasingly been implanted to stabilize the pelvic floor, which has led to considerable improvement of anatomical results. But the potential for mesh-induced risks has led to a controversial discussion on the use of surgical meshes in urogynecology. The impact of cystocele correction and implantation of an alloplastic mesh on patientsʼ quality of life/sexuality and the long-term stability of this approach were investigated.

Method In a large prospective multicenter study, 289 patients with symptomatic cystocele underwent surgery with implantation of a titanized polypropylene mesh (TiLOOP® Total 6, pfm medical ag) and followed up for 36 months. Both primary procedures and procedures for recurrence were included in the study. Anatomical outcomes were quantified using the POP-Q system. Quality of life including sexuality were assessed using the German version of the validated P-QoL questionnaire. All adverse events were assessed by an independent clinical event committee.

Results Mean patient age was 67 ± 8 years. Quality of life improved significantly over the course of the study in all investigated areas, including sexuality and personal relationships (p < 0.001, Wilcoxon test). The number of adverse events which occurred in the period between 12 and 36 months after surgery was low, with just 22 events reported. The recurrence rate for the anterior compartment was 4.5%. Previous or concomitant hysterectomy increased the risk of recurrence in the posterior compartment 2.8-fold and increased the risk of erosion 2.25-fold.

Conclusion Cystocele correction using a 2nd generation alloplastic mesh achieved good anatomical and functional results in cases requiring stabilization of the pelvic floor and in patients with recurrence. The rate of recurrence was low, the patientsʼ quality of life improved significantly, and the risks were acceptable.
Differential shrinkage induced formation of yolk-shell carbon microspheres toward enhanced microwave absorption
Authors: Tian Chunhua , Du Yunchen , Xu Haiyan , Xue Jianlei , Chu Wenlei , Qiang Rong , Han Xijiang , Xu Ping
Published: September 2017

Rational design of the microstructure paves new ways for microwave absorbing materials because it can create more facilities for the attenuation of incident electromagnetic waves. In this study, a simple method is proposed to prepare yolk-shell carbon microspheres through differential shrinkage in the internal cores and external shells of polypyrrole microspheres with the assistance of outermost SiO2 coating. This method simplifies the preparation procedures and avoids strictly controlled conditions. The electromagnetic parameters, such as relative complex permittivity and permeability, of the as-prepared yolk-shell carbon microspheres, are investigated in the frequency range of 2.0–18.0 GHz. Compared with solid carbon microspheres, yolk-shell carbon microspheres exhibit significantly enhanced microwave absorption properties in terms of both the reflection loss intensity and absorption bandwidth. The minimum reflection loss value can reach up to −27.5 dB at 8.32 GHz with an absorber thickness of 2.96 mm. The absorption bandwidth over −10.0 dB is in the range of 11.3–16.2 GHz at the typical thickness of 2.0 mm. The enhanced microwave absorption properties may be attributed to the good attenuation ability and well matched characteristic impedance. This work not only provides a promising candidate for microwave absorption, but also provides an attractive strategy to prepare various yolk-shell composites.
Quantum eigenstate tomography with qubit tunneling spectroscopy
Authors: Smirnov Anatoly Yu. , Amin Mohammad H.
Published: September 2017

Measurement of the energy eigenvalues (spectrum) of a multi-qubit system has recently become possible by qubit tunneling spectroscopy (QTS). In the standard QTS experiments, an incoherent probe qubit is strongly coupled to one of the qubits of the system in such a way that its incoherent tunneling rate provides information about the energy eigenvalues of the original (source) system. In this paper, we generalize QTS by coupling the probe qubit to many source qubits. We show that by properly choosing the couplings, one can perform projective measurements of the source system energy eigenstates in an arbitrary basis, thus performing quantum eigenstate tomography. As a practical example of a limited tomography, we apply our scheme to probe the eigenstates of a kink in a frustrated transverse Ising chain.
Application and fabrication aspects of sub-micrometer-sized Josephson junctions
Authors: Oelsner G. , Hübner U. , Anders S. , Il'ichev E.
Published: September 2017

We present two possible methods for the fabrication of sub-micron sized Josephson junctions, namely the shadow-evaporation technique and the cross-type technology. Their importance for the field of modern super-conducting technology is discussed. As examples we present measurement results of a two-qubit sample and a prototype of a microwave detector fabricated each by one of the described methods. We review potential applications of superconducting quantum circuits based on the developed methods.
Anisotropic Friedel oscillations in a two-dimensional electron gas with a Rashba–Dresselhaus spin–orbit interaction
Authors: Kozlov I. V. , Kolesnichenko Yu. A.
Published: September 2017

We present a theoretical study of the spatial distribution of the local density of states (LDOS) and the local magnetization density (LMD) in the vicinity of a magnetic point-defect in a degenerate two-dimensional electron gas with a mixed Rashba-Dresselhaus spin-orbit coupling interaction (SOI). The dependence of the Friedel oscillations, which arise under these conditions, on the ratio of the SOI constants is investigated. We obtain asymptotic expressions for the oscillatory parts of the LDOS and the LMD, that are accurate for large distances from the defect. It is shown, that the Friedel oscillations are significantly anisotropic and contain several harmonics for certain ratios of the SOI constants. Period of the oscillations for directions along the symmetry axes of the Fermi contours are determined. Finally, we introduce a method for determining the values of the two SOI constants by measuring the period of the Friedel oscillations of the LDOS and the LMD for different harmonics.
Single-particle emission at finite temperatures
Authors: Moskalets Michael
Published: September 2017

The state of particles injected onto the surface of the Fermi sea depends essentially on the temperature. The pure state injected at zero temperature becomes a mixed state if injected at finite temperature. Moreover the electron source injecting a single-particle state at zero temperature may excite a multi-particle state if the Fermi sea is at finite temperature. Here I unveil a symmetry of the scattering amplitude of a source, which is sufficient to preserve a single-particle emission regime at finite temperatures if such a regime is achieved at zero temperature. I give an example and analyze the effect of temperature on time-dependent electrical and heat currents carried by a single-particle excitation.
Aperiodic-metamaterial-based absorber
Authors: Yang Quanlong , Chen Xieyu , Li Yanfeng , Zhang Xueqian , Xu Yuehong , Tian Zhen , Ouyang Chunmei , Gu Jianqiang , Han Jiaguang , Zhang Weili
Published: September 2017

The periodic-metamaterial-based perfect absorber has been studied broadly. Conversely, if the unit cell in the metamaterial-based absorber is arranged aperiodically (aperiodic-metamaterial-based absorber), how does it perform? Inspired by this, here we present a systematic study of the aperiodic-metamaterial-based absorber. By investigating the response of metamaterial absorbers based on periodic, Fibonacci, Thue-Morse, and quasicrystal lattices, we found that aperiodic-metamaterial-based absorbers could display similar absorption behaviors as the periodic one in one hand. However, their absorption behaviors show different tendency depending on the thicknesses of the spacer. Further studies on the angle and polarization dependence of the absorption behavior are also presented.
Large magnetoresistance and Fermi surface study of Sb2Se2Te single crystal
Authors: Shrestha K. , Marinova V. , Graf D. , Lorenz B. , Chu C. W.
Published: September 2017

We have studied the magnetotransport properties of a Sb2Se2Te single crystal. Magnetoresistance (MR) is maximum when the magnetic field is perpendicular to the sample surface and reaches a value of 1100% at B = 31 T with no sign of saturation. MR shows Shubnikov de Haas (SdH) oscillations above B = 15 T. The frequency spectrum of SdH oscillations consists of three distinct peaks at α = 32 T, β = 80 T, and γ = 117 T indicating the presence of three Fermi surface pockets. Among these frequencies, β is the prominent peak in the frequency spectrum of SdH oscillations measured at different tilt angles of the sample with respect to the magnetic field. From the angle dependence β and Berry phase calculations, we have confirmed the trivial topology of the β-pocket. The cyclotron masses of charge carriers, obtained by using the Lifshitz–Kosevich formula, are found to be mβ*=0.16mo and mγ*=0.63mo for the β and γ bands, respectively. The Large MR of Sb2Se2Te is suitable for utilization in electronic instruments such as computer hard discs, high field magnetic sensors, and memory devices.
Dispersion and non-reciprocal elastic wave propagation in a membrane coupled with a uniform flow
Authors: Ioppolo Tindaro , Rostami Parand
Published: September 2017

In this paper, we carry out an analytical study to investigate the dispersive and non-reciprocal properties of harmonic elastic wave propagation in a membrane on an elastic foundation. One side of the membrane is in contact with a uniform inviscid and incompressible flow. The analysis shows that the frequency spectrum and the dispersion curve are not symmetric, therefore breaking the principle of reciprocity. We show that the dynamics of the wave propagation of the system depends on the dimensionless phase velocity of the membrane and the dimensionless stiffness of the elastic foundation. The system possesses one region where the phase velocity of the propagating waves in opposite directions is different, and another where the waves travel only in one direction (directional band gap). There also exist regions in which only evanescent and spatially growing waves are excited.
Allometric relationships for vegetative growth flush in pruned stems of macadamia (Macadamia integrifolia): effects of carbohydrate source and season
Authors: Karimaei S. , Doley D. , Hanan J.
Published: September 2017

Hedging is used to control tree size in macadamia orchards, but the effects on subsequent shoot growth and floral initiation may impair fruit production. Four-year-old grafted macadamia (Macadamia integrifolia Maiden & Betche) trees were subjected to pruning of the most recent seasonal shoot in autumn and spring. Factorial combinations of treatments included girdling or not girdling near the base of the previous season’s (parent) shoot; removal of all except two, four or six leaves from the parent shoot; and defoliation or no defoliation of the shoot that appeared after pruning. Initial numbers and dimensions of flush-shoot internodes were similar for all branch treatments in autumn and for girdled branches in spring, and were smaller than half those for non-girdled stems in spring. Later flush-shoot growth depended on the number of leaves retained on the parent shoot, the presence or absence of a connection to the tree below the parent shoot, and on the season, declining with limiting growing degree-days in winter and accelerating with increasing growing degree-days in summer. In both seasons, reserves beyond the parent (previous season’s) shoot contributed the major source of carbohydrate for continuing flush-shoot growth, and particularly the growth of leaves. The combinations of seasonal conditions, shoot parameters before the commencement of flush-shoot growth, and flush-shoot morphology permit the inference of allometric relationships that can be applied to the quantitative modelling of vegetative shoot morphology and growth in macadamia.
The importance of seasonal resource selection when managing a threatened species: targeting conservation actions within critical habitat designations for the Gunnison sage-grouse
Authors: Rice M. B. , Apa A. D. , Wiechman L. A.
Published: September 2017

Context The ability to identify priority habitat is critical for species of conservation concern. The designation of critical habitat under the US Endangered Species Act 1973 identifies areas occupied by the species that are important for conservation and may need special management or protection. However, relatively few species’ critical habitats designations incorporate habitat suitability models or seasonal specificity, even when that information exists. Gunnison sage-grouse (GUSG) have declined substantially from their historical range and were listed as threatened by the US Fish and Wildlife Service (USFWS) in November 2014. GUSG are distributed into eight isolated populations in Colorado and Utah, and one population, the Gunnison Basin (GB), has been the focus of much research. Aims To provide season-specific resource selection models to improve targeted conservation actions within the designated critical habitat in the GB. Methods We utilised radio-telemetry data from GUSG captured and monitored from 2004 to 2010. We were able to estimate resource selection models for the breeding (1 April–15 July) and summer (16 July–30 September) seasons in the GB using vegetation, topographical and anthropogenic variables. We compared the seasonal models with the existing critical habitat to investigate whether the more specific seasonal models helped identify priority habitat for GUSG. Key results The predictive surface for the breeding model indicated higher use of large areas of sagebrush, whereas the predictive surface for the summer model predicted use of more diverse habitats. The breeding and summer models (combined) matched the current critical habitat designation 68.5% of the time. We found that although the overall habitat was similar between the critical habitat designation and our combined models, the pattern and configuration of the habitat were very different. Conclusions These models highlight areas with favourable environmental variables and spatial juxtaposition to establish priority habitat within the critical habitat designated by USFWS. More seasonally specific resource selection models will assist in identifying specific areas within the critical habitat designation to concentrate habitat improvements, conservation and restoration within the GB. Implications This information can be used to provide insight into the patterns of seasonal habitat selection and can identify priority GUSG habitat to incorporate into critical habitat designation for targeted management actions.
Indigenous Education and Decolonization
Authors: Poitras Pratt Yvonne , Louie Dustin W. , Hanson Aubrey Jean , Ottmann Jacqueline
Published: September 2017

The need to decolonize and Indigenize education stems from shared experiences of colonialism across the globe. In a world divided by ongoing conflict, and fueled by issues of power and control, the need to closely examine the ways that education has served hegemonic interests will help to inform future educational initiatives as well as serve as a form of reparation for those Indigenous peoples who have endured the dire consequences of colonialism. Present-day efforts to reclaim, restore, and revitalize threatened traditions are supported by international bodies such as the United Nations, in tandem with a range of approaches at national levels.

Decolonizing education entails identifying how colonization has impacted education and working to unsettle colonial structures, systems, and dynamics in educational contexts. We use the term education in these descriptions broadly to name the sociocultural task of understanding ways of knowing and being (epistemological and ontological systems) and the ongoing formation and transmission of knowledges: for instance, we mean both formal education as structured through Western schooling and other forms of education such as those traditionally practiced within Indigenous families and communities. Decolonizing education fits within larger understandings of decolonization and Indigenization at socio-political levels. However, these undertakings address in particular the colonization of the mind, of knowledge, language, and culture, and the impacts of colonization at personal and collective levels of physical, emotional, spiritual, psychological, and intellectual experience. In this time of transition, the work of decolonizing schooling necessarily precedes that of Indigenizing education for most educators and learners; yet, in keeping with Indigenous knowledge traditions, education must remain in a state of flux as we come to know this work collectively.
Professionalizing Teacher Education Accountability
Authors: Mayer Diane
Published: September 2017

This article examines teacher education accountability and argues for new emphases in accreditation and beginning teacher certification designed to professionalize teacher education. A brief overview of the history of teacher education policy is presented as a background framing for exploring the current policy moment positioning teacher education as a problem that needs to be fixed. Government responses discussed are mainly those in the Anglophone areas of Australia, North America, and the United Kingdom. These involve tighter regulation while at the same time opening up a deregulated teacher education environment as well as an increasing focus on measuring the contribution that teacher preparation makes to student learning. The article suggest ways of professionalizing teacher education accountability which go beyond the “partnerships,” “classroom-ready,” and “value-added” mantras of current debates and policies and considers (1) teacher education in a new hybrid space, (2) authentic graduate assessments, and (3) rigorous research evidence as the cornerstones of a refreshed and more professionalised approach to teacher education accountability.
How large are the globular cluster systems of early-type galaxies and do they scale with galaxy halo properties?
Authors: Forbes Duncan A.
Published: September 2017

The globular cluster (GC) systems of galaxies are well known to extend to large galactocentric radii. Here, we quantify the size of GC systems using the half number radius of 22 GC systems around early-type galaxies (ETGs) from the literature. We compare GC system sizes to the sizes and masses of their host galaxies. We find that GC systems typically extend to 4 times that of the host galaxy size; however, this factor varies with galaxy stellar mass from about 3 times for M* galaxies to 5 times for the most massive galaxies in the universe. The size of a GC system scales approximately linearly with the virial radius (R200) and with the halo mass (M200) to the 1/3 power. The GC system of the Milky Way follows the same relations as for ETGs. For ultra diffuse galaxies (UDGs), their GC system size scales with halo mass and virial radius as for more massive, larger galaxies. UDGs indicate that the linear scaling of GC system size with stellar mass for massive galaxies flattens out for low stellar mass galaxies. Our scalings are different to those reported recently by Hudson & Robison.
Automated influenza case detection for public health surveillance and clinical diagnosis using dynamic influenza prevalence method
Authors: Tsui Fuchiang , Ye Ye , Ruiz Victor , Cooper Gregory F , Wagner Michael M
Published: September 2017

Objectives

To assess the performance of a Bayesian case detector (BCD) for influenza surveillance and clinical diagnosis.

Methods

BCD uses a Bayesian network classifier to compute the posterior probability of a patient having influenza based on 31 findings from narrative clinical notes. To assess the potential for disease surveillance, we calculated area under the receiver operating characteristic curve (AUC) to indicate BCD’s ability to differentiate between influenza and non-influenza encounters in emergency department settings. To assess the potential for clinical diagnosis, we measured AUC for diagnosing influenza cases among encounters having influenza-like illnesses. We also evaluated the performance of BCD using dynamically estimated influenza prevalence, and measured sensitivity, specificity and positive predictive value.

Results

For influenza surveillance, BCD differentiated between influenza and non-influenza encounters well with an AUC of 0.90 and 0.97 with dynamic influenza prevalence (P < 0.0001). For clinical diagnosis, the addition of dynamic influenza prevalence to BCD significantly improved AUC from 0.63 to 0.85 to distinguish influenza from other causes of influenza-like illness.

Conclusions and policy implications

BCD can serve as an influenza surveillance and a differential diagnosis tool via our dynamic prevalence approach. It enhances the communication between public health and clinical practice.
Identification of Gene Candidates Associated with Huanglongbing Tolerance, Using ‘Candidatus Liberibacter asiaticus’ Flagellin 22 as a Proxy to Challenge Citrus
Authors: Shi Qingchun , Febres Vicente J. , Zhang Shujian , Yu Fahong , McCollum Greg , Hall David G. , Moore Gloria A. , Stover Ed
Published: September 2017

The 22–amino acid (flg22) pathogen-associated molecular pattern from the flagellin of Xanthomonas citri subsp. citri has been shown to induce defense responses correlated with citrus canker resistance. Here, flg22 of ‘Candidatus Liberibacter asiaticus’, the putative causal agent of Huanglongbing (HLB), elicited differential defense responses that were weaker than those from Xcc-flg22, between those of the HLB-tolerant mandarin cultivar Sun Chu Sha and susceptible grapefruit cultivar Duncan. Transcriptomics was used to compare the effect of CLas-flg22 and Xcc-flg22 between the citrus genotypes and identified 86 genes induced only by CLas-flg22 in the tolerant mandarin. Expression of 16 selected genes was validated, by reverse transcription-quantitative polymerase chain reaction, and was evaluated in citrus during ‘Ca. L. asiaticus’ infection. Differential expression of a number of genes occurred between tolerant and susceptible citrus infected with ‘Ca. L. asiaticus’, suggesting their involvement in HLB tolerance. In addition, several genes were similarly regulated by CLas-flg22 and ‘Ca. L. asiaticus’ treatments, while others were oppositely regulated in the tolerant mandarin, suggesting similarity and interplay between CLas-flg22 and ‘Ca. L. asiaticus’–triggered defenses. Genes identified are valuable in furthering the study of HLB tolerance mechanisms and, potentially, for screening for HLB-tolerant citrus using CLas-flg22 as a pathogen proxy.
A Gene Family Coding for Salivary Proteins (SHOT) of the Polyphagous Spider Mite Tetranychus urticae Exhibits Fast Host-Dependent Transcriptional Plasticity
Authors: Jonckheere Wim , Dermauw Wannes , Khalighi Mousaalreza , Pavlidi Nena , Reubens Wim , Baggerman Geert , Tirry Luc , Menschaert Gerben , Kant Merijn R. , Vanholme Bartel , Van Leeuwen Thomas
Published: September 2017

The salivary protein repertoire released by the herbivorous pest Tetranychus urticae is assumed to hold keys to its success on diverse crops. We report on a spider mite–specific protein family that is expanded in T. urticae. The encoding genes have an expression pattern restricted to the anterior podocephalic glands, while peptide fragments were found in the T. urticae secretome, supporting the salivary nature of these proteins. As peptide fragments were identified in a host-dependent manner, we designated this family as the SHOT (secreted host–responsive protein of Tetranychidae) family. The proteins were divided in three groups based on sequence similarity. Unlike TuSHOT3 genes, TuSHOT1 and TuSHOT2 genes were highly expressed when feeding on a subset of family Fabaceae, while expression was depleted on other hosts. TuSHOT1 and TuSHOT2 expression was induced within 24 h after certain host transfers, pointing toward transcriptional plasticity rather than selection as the cause. Transfer from an ‘inducer’ to a ‘noninducer’ plant was associated with slow yet strong downregulation of TuSHOT1 and TuSHOT2, occurring over generations rather than hours. This asymmetric on and off regulation points toward host-specific effects of SHOT proteins, which is further supported by the diversity of SHOT genes identified in Tetranychidae with a distinct host repertoire.
Suppression or Activation of Immune Responses by Predicted Secreted Proteins of the Soybean Rust Pathogen Phakopsora pachyrhizi
Authors: Qi Mingsheng , Grayczyk James P. , Seitz Janina M. , Lee Youngsill , Link Tobias I. , Choi Doil , Pedley Kerry F. , Voegele Ralf T. , Baum Thomas J. , Whitham Steven A.
Published: September 2017

Rust fungi, such as the soybean rust pathogen Phakopsora pachyrhizi, are major threats to crop production. They form specialized haustoria that are hyphal structures intimately associated with host-plant cell membranes. These haustoria have roles in acquiring nutrients and secreting effector proteins that manipulate host immune systems. Functional characterization of effector proteins of rust fungi is important for understanding mechanisms that underlie their virulence and pathogenicity. Hundreds of candidate effector proteins have been predicted for rust pathogens, but it is not clear how to prioritize these effector candidates for further characterization. There is a need for high-throughput approaches for screening effector candidates to obtain experimental evidence for effector-like functions, such as the manipulation of host immune systems. We have focused on identifying effector candidates with immune-related functions in the soybean rust fungus P. pachyrhizi. To facilitate the screening of many P. pachyrhizi effector candidates (named PpECs), we used heterologous expression systems, including the bacterial type III secretion system, Agrobacterium infiltration, a plant virus, and a yeast strain, to establish an experimental pipeline for identifying PpECs with immune-related functions and establishing their subcellular localizations. Several PpECs were identified that could suppress or activate immune responses in nonhost Nicotiana benthamiana, N. tabacum, Arabidopsis, tomato, or pepper plants.
Trick or Treat: Microbial Pathogens Evolved Apoplastic Effectors Modulating Plant Susceptibility to Infection
Authors: Wang Yan , Wang Yuanchao
Published: September 2017

The apoplastic space between the plant cell wall and the plasma membrane constitutes a major battleground for plant-pathogen interactions. To survive in harsh conditions in the plant apoplast, pathogens must cope with various immune responses. During infection, plant pathogens secrete an arsenal of effector proteins into the apoplast milieu, some of which are detected by the plant surveillance system and, thus, activate plant innate immunity. Effectors that evade plant perception act in modulating plant apoplast immunity to favor successful pathogen infection. The concerted actions of apoplastic effectors often determine the outcomes of plant-pathogen interactions. In this review, we summarize current advances on the understanding of apoplastic effectors and highlight the strategies employed by pathogens to counter host apoplastic defense.
Characterization of Pepper leafroll chlorosis virus, a New Polerovirus Causing Yellowing Disease of Bell Pepper in Saudi Arabia
Authors: Kamran A. , Lotos L. , Amer M. A. , Al-Saleh M. A. , Alshahwan I. M. , Shakeel M. T. , Ahmad M. H. , Umar M. , Katis N. I.
Published: September 2017

During the growing seasons of 2014 through 2016, a total of 336 leaf samples from bell pepper (showing leafroll and interveinal yellowing) and arable weeds were collected from Riyadh region, Saudi Arabia. The use of a polerovirus generic reverse transcription (RT)-PCR assay confirmed their presence in the bell pepper samples. Sequencing of the generic amplicon revealed high similarity (87.6 to 98.1% in nt) with four poleroviruses; Tobacco vein distorting virus, Pepper vein yellows virus, Pepper yellows virus, and Pepper yellow leaf curl virus. To further characterize one of these isolates (105D), a larger part of the genome (∼1,300 nt) spanning approximately from the 3′ end of ORF2 to the middle of ORF3, was amplified and sequenced. Blasting the resulting sequence revealed the low amino acid and nucleotide identity percentages in the coat protein and movement protein partial genes with viruses deposited in GenBank. Next-generation sequence was used to acquire a larger part of the genome, which resulted in the reconstruction of isolate 105D’s partial genome (5,496 nt). Sequence similarity analysis revealed the presence of a divergent polerovirus isolate belonging to a new species that was tentatively named Pepper leafroll chlorosis virus (PeLRCV). Using a specific RT-PCR assay for this isolate confirmed the presence of this new viral species in the symptomatic peppers. Aphid transmission experiments showed that PeLRCV is vectored by Aphis gossypii and that it can infect at least five out of the 15 different plants species tested. Based on our findings, PeLRCV is a new member of genus Polerovirus in the family Luteoviridae.
Variation withinLactucaspp. for Resistance toImpatiens necrotic spot virus
Authors: Simko Ivan , Richardson Claire E. , Wintermantel William M.
Published: September 2017

Lettuce (Lactuca sativa L.) production in coastal California, one of the major lettuce-producing areas of the United States, is regularly affected by outbreaks of Impatiens necrotic spot virus (INSV), a member of the genus Orthotospovirus. Transmission of INSV among lettuce crops in this growing region has been attributed predominantly to the western flower thrips (Frankliniella occidentalis). INSV is acquired by first- or second-instar thrips nymphs feeding on infected host plants (not necessarily lettuce). The virus replicates within the insect vector, and is transmitted to new plants by adult thrips as they feed on epidermal and mesophyll cells of susceptible host plants. All currently grown cultivars of lettuce are susceptible to the disease. Screening lettuce for resistance to INSV under field conditions is problematic because natural infections appear sporadically and the virus is not evenly distributed across infected fields. We have developed a greenhouse-based assay that uses viruliferous thrips in combination with mechanical inoculation that allows dependable, year-round screening for resistance. In all, 89 cultivars, breeding lines, and plant introductions of cultivated lettuce, together with 53 accessions from 11 other Lactuca spp., 4 accessions from two dandelion (Taraxacum) species, and 4 tomato (Solanum lycopersicum L.) lines were evaluated for resistance to INSV. All tested material was susceptible to INSV to varying degrees, with the exception of two tomato lines that carry the Sw-5 gene that confers resistance to Tomato spotted wilt virus, a virus closely related to INSV. In cultivated lettuce, a partial resistance to INSV was observed in cultivars Amazona, Ancora, Antigua, Commodore, Eruption, Iceberg, La Brillante, Merlot, Telluride, and Tinto. Limited comparison of the greenhouse-based screening results with the data from opportunistic evaluations of resistance on 775 lettuce accessions from six field trials indicates consistency of results from both greenhouse and field environments. The most resistant lettuce accessions are being incorporated into our breeding program for introgression of resistance into lettuce breeding lines.
Virulence of Egyptian Blumeria graminis f. sp. tritici Population and Response of Egyptian Wheat Cultivars
Authors: Abdelrhim Abdelrazek , Abd-Alla Harby M. , Abdou El-Sayed , Ismail Mamdoh E. , Cowger Christina
Published: September 2017

Powdery mildew, caused by Blumeria graminis (DC.) Speer f. sp. tritici (Em. Marchal), is a serious disease of wheat that can cause a large reduction in yield. In Egypt, high powdery mildew severity has been observed in the past few years on many commercial cultivars of both bread and durum wheat. Little information is available about virulence characteristics of the Egyptian B. graminis f. sp. tritici population in Egypt or the resistance of Egyptian wheat cultivars to powdery mildew. Virulence frequencies of a representative sample of the Egyptian B. graminis f. sp. tritici population were studied. Seven provinces were chosen to represent the country: two in Upper Egypt (Qena and Sohag), one in Middle Egypt (El Minia), and four in the north (Alexandria, Kafr Elsheikh, Dakahlia, and Sharqia). Ten isolates from each province (70 isolates total) were derived from single ascospores and used for this study. They were inoculated individually on 21 powdery mildew differential lines, each bearing a single resistance (Pm) gene. Also, the responses of 14 Egyptian bread wheat cultivars and 6 durum cultivars to each of the 70 isolates were evaluated individually. Among all tested Pm genes, only seven (Pm1b, Pm2, Pm21, Pm34, Pm36, Pm37, and Pm53) were effective against B. graminis f. sp. tritici isolates from all provinces. Several other genes were effective against most or all isolates from a majority of provinces. All tested bread wheat cultivars showed full susceptibility to all isolates, whereas two durum wheat cultivars, Beni-Suef-5 and Beni-Suef-6, had intermediate responses to a large percentage of the isolates, likely indicating partial resistance. To enhance mildew resistance in Egyptian wheat cultivars, it is recommended to use combinations of genes that are nationally effective or effective against multiple provincial B. graminis f. sp. tritici populations.
Analysis of Genetic Diversity of Russian Sour Cherry Plum pox virus Isolates Provides Evidence of a New Strain
Authors: Chirkov Sergei , Sheveleva Anna , Ivanov Peter , Zakubanskiy Alexander
Published: September 2017

Plum pox virus (PPV) exists as a complex of nine strains adapted to different Prunus hosts. Unusual PPV isolates that do not belong to the known cherry-adapted strains were discovered on sour cherry in Russia. Here, two complete genomes of isolates Tat-2 and Tat-4 were determined by sequencing on the Illumina HiSeq 2500 platform. Both were composed of 9,792 nucleotides, excluding the poly(A) tail, with the organization typical of PPV and had 99.4 and 99.7% identity between each other at the nucleotide and amino acid levels. The sequence identities between Tat-2/Tat-4 and known PPV strains ranged from 77.6 to 83.3% for genomic RNA and from 80.0 to 93.8% for polyprotein. Phylogenetic analysis placed Tat-2 and Tat-4 in a separate clade, distinct from the C and CR strains. Three more Tat-2/Tat-4-like isolates were detected in local cherry plantings using the newly developed, specific RT-PCR assay. Based on the phylogenetic analysis, sequence identities, and environmental distribution, Tat-2, Tat-4, and related isolates represent a new cherry-adapted PPV strain for which the name PPV-CV (Cherry Volga) is proposed.
Contrast Between Orange- and Black-Colored Sclerotial Isolates of Botrytis cinerea: Melanogenesis and Ecological Fitness
Authors: Zhou Yingjun , Li Na , Yang Jingyi , Yang Long , Wu Mingde , Chen Weidong , Li Guoqing , Zhang Jing
Published: September 2017

Botrytis cinerea usually produces grayish mycelia and conidia as well as black-colored sclerotia (BS) due to accumulation of melanin. An isolate (XN-1) of B. cinerea producing orange-colored sclerotia (OS) on agar media was obtained from an orange-colored apothecium of an uncultured soil fungus. Whether or not the OS B. cinerea occurs on plants and how they differ from the BS isolates in melanogensis and ecological fitness remained unknown. This study, for the first time, confirmed the presence of the OS B. cinerea in strawberry and tomato plants that were surveyed in Hubei Province of China. Only five OS isolates were obtained from a total of 2,031 isolates surveyed from the two crops. The OS isolate XN-1 was compared and contrasted with the BS isolate B05.10 in sclerotial melanogenesis and ecological fitness. Sclerotial melanogenesis was evident in B05.10 but was deficient in XN-1. The OS were more susceptible to the two mycoparasites Trichoderma koningiopsis and Clonostachys rosea than the BS. The percentage of viable sclerotia after the mycoparasitism study was significantly (P < 0.01) lower in OS (21%) than in BS (48%). This study also reaffirmed the importance of melanization for survival of B. cinerea sclerotia.
Multiple Nodulation Genes Are Up-Regulated During Establishment of Reniform Nematode Feeding Sites in Soybean
Authors: Redding Nathan W. , Agudelo Paula , Wells Christina E.
Published: September 2017

The semi-endoparastic reniform nematode (Rotylenchulus reniformis) infects over 300 plant species. Females penetrate host roots and induce formation of complex, multinucleate feeding sites called syncytia. While anatomical changes associated with reniform nematode infection are well documented, little is known about their molecular basis. We grew soybean (Glycine max) in a split-root growth system, inoculated half of each root system with R. reniformis, and quantified gene expression in infected and control root tissue at four dates after inoculation. Over 6,000 genes were differentially expressed between inoculated and control roots on at least one date (false discovery rate [FDR] = 0.01, |log2FC| ≥ 1), and 507 gene sets were significantly enriched or depleted in inoculated roots (FDR = 0.05). Numerous genes up-regulated during syncytium formation had previously been associated with rhizobia nodulation. These included the nodule-initiating transcription factors CYCLOPS, NSP1, NSP2, and NIN, as well as multiple nodulins associated with the plant-derived peribacteroid membrane. Nodulation-related NIP aquaporins and SWEET sugar transporters were induced, as were plant CLAVATA3/ESR-related (CLE) signaling proteins and cell cycle regulators such as CCS52A and E2F. Nodulins and nodule-associated genes may have ancestral functions in normal root development and mycorrhization that have been co-opted by both parasitic nematodes and rhizobial bacteria to promote feeding site and nodule formation.
Insights Into the Ecology of Grapevine red blotch virus in a Diseased Vineyard
Authors: Cieniewicz Elizabeth J. , Pethybridge Sarah J. , Loeb Gregory , Perry Keith , Fuchs Marc
Published: September 2017

Limited information is available on the spread of Grapevine red blotch virus (GRBV, genus Grablovirus, family Geminiviridae) in vineyards. To investigate ecological aspects of red blotch disease spread, sticky cards to catch flying insects were placed in 2015 (April to November) and 2016 (March to November) in a vineyard study site in California where disease incidence increased by nearly 20% between 2014 and 2016. Subsets of insect species or taxa were removed from sticky card traps and individual specimens were tested for the presence of GRBV by multiplex polymerase chain reaction. GRBV was consistently detected in Spissistilus festinus (Membracidae), Colladonus reductus (Cicadellidae), Osbornellus borealis (Cicadellidae), and a Melanoliarus sp. (Cixiidae). Populations of these four candidate vectors peaked from June to September, with viruliferous S. festinus peaking from late June to early July in both years. An assessment of co-occurrence and covariation between the spatial distribution of GRBV-infected vines and viruliferous insects identified a significant association only with viruliferous S. festinus. These findings revealed the epidemiological relevance of S. festinus as a vector of GRBV in a vineyard ecosystem. Sequencing coat protein and replicase-associated protein gene fragments of GRBV isolates from newly infected vines and viruliferous vector candidates further suggested secondary spread primarily from local sources and occasionally from background sources.
Tranexamic Acid in Orthopaedic Trauma Surgery: A Meta-Analysis
Authors: Gausden Elizabeth B. , Qudsi Rameez , Boone Myles D. , O'Gara Brian , Ruzbarsky Joseph J. , Lorich Dean G.
Published: September 2017

Aim:

To systematically review and quantify the efficacy of tranexamic acid (TXA) use in reducing the risk of receiving a blood transfusion in patients undergoing orthopaedic trauma surgery, in reducing blood loss, and risk of thromboembolic events.

Methods:

A systematic literature search was performed using MEDLINE, Embase, ClinicalTrials.gov, and conference proceeding abstracts from 2014 to 2016. A minimum of 2 reviewers screened each study and graded quality. The primary outcome measure was the risk of receiving a blood transfusion in the TXA group versus control. A meta-analysis was performed to construct a combined odds ratio (OR) of receiving a blood transfusion, mean difference (MD) of blood loss, and OR of thromboembolic events.

Results:

Twelve studies were included in the quantitative analysis (1,333 patients). The risk of blood transfusion was significantly less in patients who were administered TXA compared with controls [OR 0.407; 95% confidence interval (CI) 0.278–0.594, I2 = 34, Q = 17, P ≤ 0.001]. There was significantly less blood loss in the TXA group compared with controls, as the mean difference was 304 mL (95% CI, 142–467 mL) (I2 = 94, Q value = 103, P < 0.001). There was no significant difference in risk of symptomatic thromboembolic events (OR 0.968; 95% CI, 0.530–1.766, I2 = 0, Q value = 5, P = 0.684).

Conclusions:

In patients with orthopaedic trauma, TXA reduces the risk of blood transfusion, reduces perioperative blood loss, and has no significant effect on the risk of symptomatic thromboembolic events. More high-quality studies are needed to ensure the safety of the drug in these patients.

Level of Evidence:

Therapeutic Level I. See Instructions for Authors for a complete description of levels of evidence.
Induced Membrane Technique: Pearls and Pitfalls
Authors: Masquelet Alain C.
Published: September 2017

The induced membrane technique for reconstruction of bone defects has proved its effectiveness and is now widely accepted. The 2 stages procedure, implying a cement spacer in the first stage and a huge bone graft in the second stage, responds to rigorous technical details involving each phase of the procedure. Prerequisites for the technique, cement spacer, bone fixation, time between the 2 stages, grafting, and postoperative course are reported and discussed.
Preoperative Evaluation and Optimization for Reconstruction of Segmental Bone Defects of the Tibia
Authors: McClure Philip K. , Alrabai Hamza M. , Conway Janet D.
Published: September 2017

Reconstruction of segmental bone defects requires a large commitment both on the part of the patient and the physician. Investing in preoperative evaluation and optimization is the only logical way to pursue such an endeavor. Unfortunately, detailed studies regarding segmental bone defects and preoperative factors are relatively lacking owing to the relatively low incidence of the problem. Fortunately, other orthopaedic pathologies (arthritis, ligamentous injuries about the knee) have high prevalence and consistency, allowing detailed analysis of preoperative factors. We review this literature, and that directly involving segmental bone defects when available, to guide surgeons planning segmental bone defect reconstruction.
“Iatrogenic” Segmental Defect: How I Debride High-Energy Open Tibial Fractures
Authors: Southam Brendan R. , Archdeacon Michael T.
Published: September 2017

High-energy, open tibial shaft fractures may result in significant comminution, bone loss, and soft tissue injuries. Early, thorough debridement of all nonviable tissue is of critical importance in treating these fractures as an inadequate initial debridement increases the risk of infection and nonunion. Large iatrogenic bone and soft tissue defects can result from debridement and will require subsequent reconstruction by both orthopaedic and plastic surgeons. Although a variety of approaches exist to address these reconstructions, successful management of bone defects remains a considerable challenge. In this article, we detail our approach to debridement and reconstruction of segmental tibial defects and provide a review on the literature on this topic.
Ilizarov Treatment Protocols in the Management of Infected Nonunion of the Tibia
Authors: McNally Martin , Ferguson Jamie , Kugan Raj , Stubbs David
Published: September 2017

Objectives:

We present a treatment algorithm comprising 4 Ilizarov methods in managing infected tibial nonunion, using nonunion mobility and segmental defect size to govern treatment choice.

Design:

Decision protocol analysis study.

Setting:

A university-affiliated teaching hospital.

Patients/Participants:

Seventy-nine patients were treated with 1 of 4 Ilizarov protocols. All patients had undergone at least one previous operation, 38 had associated limb deformity, and 49 had nonviable nonunions. Twenty-six had a new muscle flap at the time of Ilizarov surgery, and 25 had preexisting flaps reused.

Intervention:

Twenty-six cases were treated with monofocal distraction, 19 with monofocal compression, 16 with bifocal compression/distraction, and 18 with bone transport.

Main Outcome Measurements:

The primary outcome measure was the absence of recurrent infection. Secondary outcomes included bone union, complications, the Association for the Advancement of Methods of Ilizarov (ASAMI) bone and functional classification scores, and any need for further unplanned surgery.

Results:

Infection was eradicated in 76 cases (96.2%) with a mean follow-up duration of 40.8 months (range 6–131). All 3 infection recurrences occurred in the monofocal compression group. Following the initial Ilizarov method alone, union was achieved in 68 cases (86.1%) and was highest among the monofocal distraction (96.2%) and bifocal compression/distraction groups (93.8%). Monofocal compression achieved the lowest union rate (73.7%), significantly lower ASAMI scores, and a refracture rate of 31.6%. Bone transport secured union in 77.8% with a 44.4% unplanned reoperation rate. However, infection-free union was 100% after further treatment.

Conclusions:

Monofocal compression is not recommended for treating infected, mobile nonunions. Distraction (monofocal or bifocal) was effective and achieved higher rates of union and infection clearance.

Level of Evidence:

Level III.