bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the Effectiveness of Pod Sealants in Increasing Pod Shattering Resistance in Oilseed Rape (Brassica napus L.)
Authors: Steponavičius Dainius , Kemzūraitė Aurelija , Bauša Laimis , Zaleckas Ernestas
Published: June 2019

Shattering of pods of oilseed rape (Brassica napus L.) is a major cause of seed yield losses prior to and during harvesting. In order to reduce shattering, researchers have been engaged in the development of special preparations that are known as pod sealants (PS). Despite the fact that there are already developed and commercialized PSs that have only been effective on seed yield preservation under certain environmental conditions, there is still a need to create a more versatile and efficient PS. Currently, the most promising method of controlling pod shattering in oilseed rape is the application of our developed novel acrylic- and trisiloxane-based pod sealant (PS4). The effectiveness of PS4 and three commercial pod sealants (PS1, PS2, and PS3) was assessed in this comparative study. By spraying an oilseed rape crop with PS4, natural seed loss can be reduced by 20–70%, depending on the prevailing weather conditions, and loss of seeds during harvest can be reduced by more than three-fold compared with that by the control treatment. Thus, the overall results demonstrated that by applying a novel pod sealant (PS4) to oilseed rape crops 2 weeks before harvest can increase the net profit margin by €30–€150 ha−1. The life cycle assessment showed that during 2014–2016 oilseed rape cultivation, the largest effect on global warming emission (kg CO2 eq) reduction was experimental sealant PS4, i.e., approximately 17% compared to the control.
A Current-to-Voltage DC-DC Converter for Powering Backbone Devices of Scientific Cabled Seafloor Observatories
Authors: Zhu Jiayu , Lyu Feng
Published: June 2019

In scientific cabled seafloor observatories, branching units and optical repeaters are essential backbone devices, in which zener diodes are commonly used in their power supply circuits. However, the low efficiency of zener-diode-based power feeding modules under large currents makes for a significant threat to the long-term reliability of backbone devices. In this paper, a novel full-bridge DC-DC converter with a duty-cycle-overlap control (DCOC) strategy is proposed to achieve high-efficiency current-to-voltage conversion. The circuit design of the converter and the principle of the DCOC strategy are analyzed. A prototype of the converter is implemented to demonstrate the feasibility of the proposed power feeding approach for large-scale cabled seafloor observatories.
Forecast Horizon and Solar Variability Influences on the Performances of Multiscale Hybrid Forecast Model
Authors: Monjoly Stéphanie , André Maina , Calif Rudy , Soubdhan Ted
Published: June 2019

The tropical insular region is characterized by a large diversity of microclimates and land/sea contrasts, creating a challenging solar forecasting. Therefore, it is necessary to develop and use performant and robustness forecasting techniques. This paper examines the predictive performance of a novel solar forecasting approach, the multiscale hybrid forecast model (MHFM), as a function of several parameters. The MHFM model is a technique recently used for irradiance forecasting based on a hybrid autoregressive (AR) and neural network (NN) model combined with multiscale decomposition methods. This technique presents a relevant performance for 1 h ahead global horizontal irradiance forecast. The goal of this work is to highlight the strength and limits of this model by assessing the influence of different parameters from a metric error analysis. This study illustrates modeling process performance as a function of daily insolation conditions and testifies the influence of learning data and test data time scales. Several forecast horizon strategies and their influence on the MHFM performance were investigated. With the best strategy, a rRMSE value from 4.43 % to 10.24 % was obtained for forecast horizons from 5 min to 6 h. The analysis of intra-day solar resource variability showed that the best performance of MHFM was obtained for clear sky days with a rRMSE of 2.91 % and worst for cloudy sky days with a rRMSE of 6.73 % . These works constitute an additional analysis in agreement with the literature about influence of daily insolation conditions and horizons time scales on modeling process.
A Comparative Analysis of House Owners in Need of Energy Efficiency Measures but with Different Intentions
Authors: Baumhof Robert , Decker Thomas , Menrad Klaus
Published: June 2019

Existing private homes in Germany and throughout Europe often are in need of energy efficient refurbishment measures (EERMs). However, these EERMs are not realized on the required level in order to achieve environment-related political targets. Therefore we investigate, based on an online survey of 1085 German owner-occupiers, the factors that differentiate two groups of single- and two-family house owners in need of EERM. Using an extended version of the Theory of Planned Behavior as a research framework, the performed logistic regression analysis shows that e.g., behavioral beliefs are significant factors for differentiating “Future-Refurbishers” from “Non-Refurbishers”. Based on our results we suggest an enhancement of practice-orientated initiatives, e.g., refurbishment workshops or best-practice presentations. By presenting the aesthetic appearance of refurbished buildings or providing knowledge, other owner-occupiers could be motivated to engage in EERM. In addition to funding programs, initiatives like this can be used to increase the general energy efficiency of buildings and specifically of those in cities and urban districts, where a high share of the mentioned houses is located and greenhouse gas emissions are caused to a great extent.
Noninferior Solution Grey Wolf Optimizer with an Independent Local Search Mechanism for Solving Economic Load Dispatch Problems
Authors: Xu Jianzhong , Yan Fu , Yun Kumchol , Su Lifei , Li Fengshu , Guan Jun
Published: June 2019

The economic load dispatch (ELD) problem is a complex optimization problem in power systems. The main task for this optimization problem is to minimize the total fuel cost of generators while also meeting the conditional constraints of valve-point loading effects, prohibited operating zones, and nonsmooth cost functions. In this paper, a novel grey wolf optimization (GWO), abbreviated as NGWO, is proposed to solve the ELD problem by introducing an independent local search strategy and a noninferior solution neighborhood independent local search technique to the original GWO algorithm to achieve the best problem solution. A local search strategy is added to the standard GWO algorithm in the NGWO, which is called GWOI, to search the local neighborhood of the global optimal point in depth and to guarantee a better candidate. In addition, a noninferior solution neighborhood independent local search method is introduced into the GWOI algorithm to find a better solution in the noninferior solution neighborhood and ensure the high probability of jumping out of the local optimum. The feasibility of the proposed NGWO method is verified on five different power systems, and it is compared with other selected methods in terms of the solution quality, convergence rate, and robustness. The compared experimental results indicate that the proposed NGWO method can efficiently solve ELD problems with higher-quality solutions.
Phytostabilization of Zn and Cd in Mine Soil Using Corn in Combination with Biochars and Manure-Based Compost
Authors: Sigua Gilbert C. , Novak Jeff M. , Watts Don W. , Ippolito Jim A. , Ducey Thomas F. , Johnson Mark G. , Spokas Kurt A.
Published: June 2019

Mining activities could produce a large volume of spoils, waste rocks, and tailings, which are usually deposited at the surface and become a source of metal pollution. Phytostabilization of the mine spoils could limit the spread of these heavy metals. Phytostabilization can be enhanced by using soil amendments such as manure-based biochars capable of immobilizing metal(loid)s when combined with plant species that are tolerant of high levels of contaminants while simultaneously improving properties of mine soils. However, the use of manure-based biochars and other organic amendments for mine spoil remediation are still unclear. In this greenhouse study, we evaluated the interactive effect of biochar additions (BA) with or without the manure-based compost (MBC) on the shoots biomass (SBY), roots biomass (RBY), uptake, and bioconcentration factor (BCF) of Zn and Cd in corn (Zea mays L.) grown in mine soil. Biochar additions consisting of beef cattle manure (BCM); poultry litter (PL); and lodge pole pine (LPP) were applied at 0, 2.5, and 5.0% (w/w) in combination with different rates (0, 2.5, and 5.0%, w/w) of MBC, respectively. Shoots and roots uptake of Cd and Zn were significantly affected by BA, MBC, and the interaction of BA and MBC. Corn plants that received 2.5% PL and 2.5% BCM had the greatest Cd and Zn shoot uptake, respectively. Corn plants with 5% BCM had the greatest Cd and Zn root uptake. When averaged across BA, the greatest BCF for Cd in the shoot of 92.3 was from the application of BCM and the least BCF was from the application of PL (72.8). Our results suggest that the incorporation of biochar enhanced phytostabilization of Cd and Zn with concentrations of water-soluble Cd and Zn lowest in soils amended with manure-based biochars while improving the biomass productivity of corn. Overall, the phytostabilization technique and biochar additions have the potential to be combined in the remediation of heavy metals polluted soils.
Editorial for Special Issue “Mineral Fibres”
Authors: Bloise Andrea , Punturo Rosalda , Kusiorowski Robert , Pereira Gómez Dolores
Published: June 2019

In the past 30 years, there has been a growing concern regarding the health risks of exposure to asbestos-containing materials (ACMs) and naturally occurring asbestos (NOA) [...]
A Life Cycle Perspective to Assess the Environmental and Economic Impacts of Innovative Technologies in Extra Virgin Olive Oil Extraction
Authors: Stillitano Teodora , Falcone Giacomo , De Luca Anna Irene , Piga Antonio , Conte Paola , Strano Alfio , Gulisano Giovanni
Published: June 2019

Advances in the adoption of technological innovations represent a great driver to improve the competitiveness of the Italian extra virgin olive oil (EVOO) industry. This work assesses the efficiency of an innovative extraction plant (with low oxidative impact, heating of paste before malaxation and a special decanter that avoids the final vertical centrifugation) in terms of oil yield and quality, and economic and environmental impacts. Economic and environmental impacts were evaluated by using both life cycle costing and life cycle assessment methodologies. A sensitivity analysis was also performed to highlight the uncertain factors that may strongly affect the results. Findings showed that olive milling with the innovative plant resulted in olive oil with a significant increase in quality, although the extraction yield was significantly higher when using conventional technology. In terms of environmental results, an average growth of 4.5% of the impacts in all categories was reached. The economic results revealed the highest extraction cost for the innovative scenario as well as the lower profitability, although a positive return in investment feasibility can be achieved due to an increase in the olive oil selling price. These findings could be useful to highlight the main hotspots in EVOO production and to suggest improvements for more sustainable management.
Artificial and Unconscious Selection in Nietzsche’s Genealogy: Expectorating the Poisoned Pill of the Lamarckian Reading
Authors: Lightbody Brian
Published: June 2019

I examine three kinds of criticism directed at philosophical genealogy. I call these substantive, performative, and semantic. I turn my attention to a particular substantive criticism that one may launch against essay two of On the Genealogy of Morals that turns on how Nietzsche answers “the time-crunch problem”. On the surface, there is evidence to suggest that Nietzsche accepts a false scientific theory, namely, Lamarck’s Inheritability Thesis, in order to account for the growth of a new human “organ”—morality. I demonstrate that the passages interpreted by some scholars to prove that Nietzsche is a Lamarckian can be reinterpreted along Darwinian lines. I demonstrate that Nietzsche hits upon the right drivers of phenotypical change in humans, namely, torture and enclosures (e.g., walls of early states), but misinterprets their true impact. Nietzsche believes that these technologies are responsible for producing what I call “culture-serving memory” and the bad conscience by causing emotions that once were expressed outwardly to turn inward causing the “psychological digestion” of the human animal. In reality, however, these mechanisms are conducive to breeding a particular type of individual, namely, one who is docile, by introducing artificial and unconscious selective pressures into the environment of early humans. In showing that Nietzsche’s genealogical account of memory and bad conscience is not underpinned on a false scientific theory and is consistent with Neo-Darwinism, I deflect a potentially fatal blow regarding the veracity of Nietzsche’s genealogies.
Single-Case Study of Appetite Control in Prader-Willi Syndrome, Over 12-Years by the Indian Extract Caralluma fimbriata
Authors: Griggs Joanne
Published: June 2019

This paper reports on the successful management of hyperphagia (exaggerated hunger) in a 14yr-old female with Prader–Willi syndrome (PWS). This child was diagnosed with PWS, (maternal uniparental disomy) at 18 months due to developmental delay, hypertonia, weight gain and extreme eating behaviour. Treatment of a supplement for appetite suppression commenced at 2 years of age. This single-case records ingestion of an Indian cactus succulent Caralluma fimbriata extract (CFE) over 12 years, resulting in anecdotal satiety, free access to food and management of weight within normal range. CFE was administered in a drink daily and dose was slowly escalated by observation for appetite suppression. Rigorous testing determined blood count, vitamins, key minerals, HbA1c, IGF-1 and function of the liver and thyroid all within normal range. The report suggests a strategy for early intervention against hyperphagia and obesity in PWS. This case was the instigator of the successful Australian PWS/CFE pilot and though anecdotal, the adolescent continues to ingest CFE followed by paediatricians at the Royal Children’s Hospital Melbourne, Victoria, Australia. Future clinical trials are worth considering, to determine an appropriate dose for individuals with PWS.
Proprietary Algorithms for Polygenic Risk: Protecting Scientific Innovation or Hiding the Lack of It?
Authors: Janssens A. Cecile J.W.
Published: June 2019

Direct-to-consumer genetic testing companies aim to predict the risks of complex diseases using proprietary algorithms. Companies keep algorithms as trade secrets for competitive advantage, but a market that thrives on the premise that customers can make their own decisions about genetic testing should respect customer autonomy and informed decision making and maximize opportunities for transparency. The algorithm itself is only one piece of the information that is deemed essential for understanding how prediction algorithms are developed and evaluated. Companies should be encouraged to disclose everything else, including the expected risk distribution of the algorithm when applied in the population, using a benchmark DNA dataset. A standardized presentation of information and risk distributions allows customers to compare test offers and scientists to verify whether the undisclosed algorithms could be valid. A new model of oversight in which stakeholders collaboratively keep a check on the commercial market is needed.
Dietary Sources of Salt in Low- and Middle-Income Countries: A Systematic Literature Review
Authors: Menyanu Elias , Russell Joanna , Charlton Karen
Published: June 2019

Rapid urbanization in low- and middle-income countries (LMICs) is transforming dietary patterns from reliance on traditional staples to increased consumption of energy-dense foods high in saturated fats, trans fats, sugars, and salt. A systematic literature review was conducted to determine major food sources of salt in LMICs that could be targeted in strategies to lower population salt intake. Articles were sourced using Medline, Web of Science, Scopus, and grey literature. Inclusion criteria were: reported dietary intake of Na/salt using dietary assessment methods and food composition tables and/or laboratory analysis of salt content of specific foods in populations in countries defined as low or middle income (LMIC) according to World Bank criteria. Of the 3207 records retrieved, 15 studies conducted in 12 LMICs from diverse geographical regions met the eligibility criteria. The major sources of dietary salt were breads, meat and meat products, bakery products, instant noodles, salted preserved foods, milk and dairy products, and condiments. Identification of foods that contribute to salt intake in LMICs allows for development of multi-faceted approaches to salt reduction that include consumer education, accompanied by product reformulation.
Altered Gene Expression in Dioxin-Like and Non-Dioxin-Like PCB Exposed Peripheral Blood Mononuclear Cells
Authors: Leijs Marike M. , Gan Lin , De Boever Patrick , Esser André , Amann Philipp M. , Ziegler Patrick , Fietkau Katharina , Schettgen Thomas , Kraus Thomas , Merk Hans F. , Baron Jens M.
Published: June 2019

Polychlorinated biphenyls (PCBs) are well known carcinogenic persistent environmental pollutants and endocrine disruptors. Our aim was to identify the possible dysregulation of genes in PCB exposed peripheral blood mononuclear cells (PBMCs) in order to give more insight into the differential pathophysiological effects of PCB congeners and mixtures, with an emphasis on immunological effects and oxidative stress. The PBMCs of a healthy volunteer (male, 56 years old) were exposed to a mixture of dioxin-like (DL)-PCBs (PCB 77, 81, 105, 114, 118, 123, 126, 156, 157, 167, 169, and 189, 250 µg/L resp.) or non-dioxin-like (NDL)-PCBs (PCB 28, 52, 101, 138, 153, 180, 250 µg/L resp.) or single PCB congener (no.28, 138, 153, 180, 250 µg/L resp.). After an incubation period of 24 h, a microarray gene expression screening was performed, and the results were compared to gene expression in control samples (PBMCs treated with the vehicle iso-octane). Treatment of PBMCs with the DL-PCB mixture resulted in the largest number of differentially regulated genes (181 upregulated genes >2-fold, 173 downregulated >2-fold). Treatment with the NDL-PCB mix resulted in 32 upregulated genes >2-fold and 12 downregulated genes >2-fold. A gene set enrichment analysis (GSEA) on DL-PCB treated PBMCs resulted in an upregulation of 125 gene sets and a downregulation of 76 gene sets. Predominantly downregulated gene sets were involved in immunological pathways (such as response to virus, innate immune response, defense response). An upregulation of pathways related to oxidative stress could be observed for all PCB congeners except PCB-28; the latter congener dysregulated the least number of genes. Our experiment augments the information known about immunological and cellular stress responses following DL- as well as NDL-PCB exposure and provides new information on PCB 28. Further studies should be performed to evaluate how disruption of these pathways contributes to the development of autoimmune diseases and cancer.
Bio-Optimization of Chemical Parameters and Earthworm Biomass for Efficient Vermicomposting of Different Palm Oil Mill Waste Mixtures
Authors: Rupani Parveen Fatemeh , Alkarkhi Abbas F. M. , Shahadat Mohammad , Embrandiri Asha , EL-Mesery Hany S. , Wang Hongcheng , Shao Weilan
Published: June 2019

The present study reports mathematical modelling of palm oil mill effluent and palm-pressed fiber mixtures (0% to 100%) during vermicomposting process. The effects of different mixtures with respect to pH, C:N ratio and earthworms have been optimized using the modelling parameters. The results of analysis of variance have established effect of different mixtures of palm oil mill effluent plus palm press fiber and time, under selected physicochemical responses (pH, C:N ratio and earthworm numbers). Among all mixtures, 60% mixture was achieved optimal growth at pH 7.1 using 16.29 C:N ratio in 15 days of vermicomposting. The relationship between responses, time and different palm oil mill waste mixtures have been summarized in terms of regression models. The obtained results of mathematical modeling suggest that these findings have potential to serve a platform for further studies in terms of kinetic behavior and degradation of the biowastes via vermicomposting.
Supervision for Certification in the Field of Applied Behaviour Analysis: Characteristics and Relationship with Job Satisfaction, Burnout, Work Demands, and Support
Authors: Dounavi Katerina , Fennell Brian , Early Erin
Published: June 2019

Background: Supervision of behavior analysts seeking certification and supervision of service delivery are key processes in the provision of quality behaviour analytic services to individuals with developmental disabilities. Our study is the first to examine international supervisory practices within the field of applied behaviour analysis. Method: An online survey was distributed to 92 professionals internationally, assessing supervisory practice, supervisor support, work demands, job satisfaction, and burnout. Results: Findings indicate high satisfaction with the supervisor and supervisory experience. Excessive work demands positively correlate with high burnout and low job satisfaction. Half of all professionals only worked with one or two clients before certification. Supervisor and collegial support seem to decrease the likelihood of suffering burnout and increase job satisfaction, although relationships were not statistically significant. Conclusions: Supervisor and collegial support warrant further research as protective factors. Implications for an evidence-based supervisory practice that produces ethical and competent supervisees are discussed.
From Neighboring Behavior to Mental Health in the Community: The Role of Gender and Work-Family Conflict
Authors: Zhang Zhenduo , Zhang Li , Zu Xiaoqian , Liu Tiansen , Zheng Junwei
Published: June 2019

This research emphasizes the potential influences of social community environments on low-income employees’ mental health. Using a two-wave panel design, we collect 218 matched data from low-income employees in Harbin City, China. We developed a moderated mediation model to test our hypotheses with the following significant results: (1) neighboring behavior, defined as both giving and receiving various kinds of assistance to and from one’s neighbors, positively influenced mental health; (2) work-family conflict mediated the relationship between neighboring behavior and mental health; (3) gender moderated the influences of neighboring behavior on mental health, such that neighboring behavior had a stronger positive influence on mental health for females than for males; (4) gender moderated the mediating effect of work-family conflict; that is, the positive influences of neighboring behavior were stronger for female employees than for male employees. This research explores the mechanism and boundary conditions of the relationship between neighboring behavior and mental health. In practice, community managers support community social workers by organizing community-building social activities and supportive programs to enhance residents’ neighboring behavior.
Two Investment Options for Bearish ETF Investors: Inverse ETF and Shorting ETF
Authors: Chovancová Božena , Dorocáková Michaela , Linnertová Dagmar
Published: June 2019

A high liquidity, low expense ratio and the possibility to conduct arbitrage allow exchange-traded funds (ETFs) to be used for short sales. Bearish investors can also buy inverse ETFs. This paper aims to outline two investment approaches for bearish ETF investors and the differences between these two approaches; it also aims to examine the relationship between price and an indicator of volume and evaluate the final positions in selected ETFs in selected periods. Short ETFs dominate in simplicity, flexibility, paying out dividends and especially in the limited size of the loss. On the other hand, their structure, which demands daily rebalancing, causes substantial deviation from the benchmark in the long-term and leads to a higher expense ratio, and lower liquidity increases bid-ask spreads. Negative aspects of ETF short selling lie in unlimited loss, high borrowing costs, the need for margin accounts, variability of loan fees and the possibility of a transaction recall by the lender. On the contrary, margin operations enable potentially higher appreciation of capital by generating rebate rates. Our results show that with the decrease in value of the most used ETFs, short interest is growing for those funds where there is a very strong negative correlation implying hedging tendencies. Short selling proved to be a more advantageous strategy in the observed period of market downturn, as well as in 2011–2017, due to negative returns, however, by applying margin trading inverse ETFs turned out to make less losses. Sector-oriented inverse ETFs are the exception, where the largest differences between these two strategies are recorded. However, the final conclusion of the suitability of one of the analyzed strategies depends on the market volatility and the direction of the market itself.
Comparative Analysis of Firearm Discharge Recorded by Gunshot Detection Technology and Calls for Service in Louisville, Kentucky
Authors: Renda William , Zhang Charlie H.
Published: June 2019

Gunshot detection technology (GDT) has been increasingly adopted by law enforcement agencies to tackle the problem of underreporting of crime via 911 calls for service, which undoubtedly affects the quality of crime mapping and spatial analysis. This article investigates the spatial and temporal patterns of gun violence by comparing data collected from GDT and 911 calls in Louisville, Kentucky. We applied hot spot mapping, near repeat diagnosis, and spatial regression approaches to the analysis of gunshot incidents and their associated neighborhood characteristics. We observed significant discrepancies between GDT data and 911 calls for service, which indicate possible underreporting of firearm discharge in 911 call data. The near repeat analysis suggests an increased risk of gunshots in nearby locations following an initial event. Results of spatial regression models validate the hypothesis of spatial dependence in frequencies of gunshot incidents and crime underreporting across neighborhoods in the study area, both of which are positively associated with proportions of African American residents, who are less likely to report a gunshot. This article adds to a growing body of research on GDT and its benefits for law enforcement activity. Findings from this research not only provide new insights into the spatiotemporal aspects of gun violence in urban areas but also shed light on the issue of underreporting of gun violence.
Dysregulated Transcriptional Control in Prostate Cancer
Authors: Baumgart Simon J. , Nevedomskaya Ekaterina , Haendler Bernard
Published: June 2019

Recent advances in whole-genome and transcriptome sequencing of prostate cancer at different stages indicate that a large number of mutations found in tumors are present in non-protein coding regions of the genome and lead to dysregulated gene expression. Single nucleotide variations and small mutations affecting the recruitment of transcription factor complexes to DNA regulatory elements are observed in an increasing number of cases. Genomic rearrangements may position coding regions under the novel control of regulatory elements, as exemplified by the TMPRSS2-ERG fusion and the amplified enhancer identified upstream of the androgen receptor (AR) gene. Super-enhancers are increasingly found to play important roles in aberrant oncogenic transcription. Several players involved in these processes are currently being evaluated as drug targets and may represent new vulnerabilities that can be exploited for prostate cancer treatment. They include factors involved in enhancer and super-enhancer function such as bromodomain proteins and cyclin-dependent kinases. In addition, non-coding RNAs with an important gene regulatory role are being explored. The rapid progress made in understanding the influence of the non-coding part of the genome and of transcription dysregulation in prostate cancer could pave the way for the identification of novel treatment paradigms for the benefit of patients.
Telomerase and Telomeres Biology in Thyroid Cancer
Authors: Donati Benedetta , Ciarrocchi Alessia
Published: June 2019

Telomere and telomerase regulation contributes to the onset and evolution of several tumors, including highly aggressive thyroid cancers (TCs). TCs are the most common endocrine malignancies and are generally characterized by a high rate of curability. However, a small but significant percentage develops distant metastasis or progresses into undifferentiated forms associated with bad prognosis and for which poor therapeutic options are available. Mutations in telomerase reverse transcriptase (TERT) promoter are among the most credited prognostic marker of aggressiveness in TCs. Indeed, their frequency progressively increases passing from indolent lesions to aggressive and anaplastic forms. TERT promoter mutations create binding sites for transcription factors, increasing TERT expression and telomerase activity. Furthermore, aggressiveness of TCs is associated with TERT locus amplification. These data encourage investigating telomerase regulating pathways as relevant drivers of TC development and progression to foster the identification of new therapeutics targets. Here, we summarize the current knowledge about telomere regulation and TCs, exploring both canonical and less conventional pathways. We discuss the possible role of telomere homeostasis in mediating response to cancer therapies and the possibility of using epigenetic drugs to re-evaluate the use of telomerase inhibitors. Combined treatments could be of support to currently used therapies still presenting weaknesses.
Genome Editing in Agriculture: Technical and Practical Considerations
Authors: Jansing Julia , Schiermeyer Andreas , Schillberg Stefan , Fischer Rainer , Bortesi Luisa
Published: June 2019

The advent of precise genome-editing tools has revolutionized the way we create new plant varieties. Three groups of tools are now available, classified according to their mechanism of action: Programmable sequence-specific nucleases, base-editing enzymes, and oligonucleotides. The corresponding techniques not only lead to different outcomes, but also have implications for the public acceptance and regulatory approval of genome-edited plants. Despite the high efficiency and precision of the tools, there are still major bottlenecks in the generation of new and improved varieties, including the efficient delivery of the genome-editing reagents, the selection of desired events, and the regeneration of intact plants. In this review, we evaluate current delivery and regeneration methods, discuss their suitability for important crop species, and consider the practical aspects of applying the different genome-editing techniques in agriculture.
Distribution and Diversity of Cytochrome P450 Monooxygenases in the Fungal Class Tremellomycetes
Authors: Akapo Olufunmilayo Olukemi , Padayachee Tiara , Chen Wanping , Kappo Abidemi Paul , Yu Jae-Hyuk , Nelson David R. , Syed Khajamohiddin
Published: June 2019

Tremellomycetes, a fungal class in the subphylum Agaricomycotina, contain well-known opportunistic and emerging human pathogens. The azole drug fluconazole, used in the treatment of diseases caused by some species of Tremellomycetes, inhibits cytochrome P450 monooxygenase CYP51, an enzyme that converts lanosterol into an essential component of the fungal cell membrane ergosterol. Studies indicate that mutations and over-expression of CYP51 in species of Tremellomycetes are one of the reasons for fluconazole resistance. Moreover, the novel drug, VT-1129, that is in the pipeline is reported to exert its effect by binding and inhibiting CYP51. Despite the importance of CYPs, the CYP repertoire in species of Tremellomycetes has not been reported to date. This study intends to address this research gap. Comprehensive genome-wide CYP analysis revealed the presence of 203 CYPs (excluding 16 pseudo-CYPs) in 23 species of Tremellomycetes that can be grouped into 38 CYP families and 72 CYP subfamilies. Twenty-three CYP families are new and three CYP families (CYP5139, CYP51 and CYP61) were conserved across 23 species of Tremellomycetes. Pathogenic cryptococcal species have 50% fewer CYP genes than non-pathogenic species. The results of this study will serve as reference for future annotation and characterization of CYPs in species of Tremellomycetes.
Vitamin D Supplementation Reduces Both Oxidative DNA Damage and Insulin Resistance in the Elderly with Metabolic Disorders
Authors: Wenclewska Sylwia , Szymczak-Pajor Izabela , Drzewoski Józef , Bunk Mariusz , Śliwińska Agnieszka
Published: June 2019

Background: Research evidence indicates that vitamin D deficiency is involved in the pathogenesis of insulin resistance (IR) and associated metabolic disorders including hyperglycemia and dyslipidemia. It also suggested that vitamin D deficiency is associated with elevated levels of oxidative stress and its complications. Therefore, the aim of our study was to determine the effect of vitamin D supplementation on DNA damage and metabolic parameters in vitamin D deficient individuals aged >45 with metabolic disorders. Material and Methods: Of 98 initially screened participants, 92 subjects deficient in vitamin D were included in the study. They were randomly assigned to the following group: with vitamin D supplementation (intervention group, n = 48) and without supplementation (comparative group, n = 44). The patients from both groups were divided into two subgroups according to the presence or absence of type 2 diabetes (T2DM). The intervention group was treated with 2000 International Unit (IU) cholecalciferol/day between October and March for three months. At baseline and after three-month supplementation vitamin D concentration (25-OH)D3 and endogenous and oxidative DNA damage were determined. In addition, fast plasma glucose (FPG), fasting insulin, HbA1c and lipid fraction (total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglyceride (TG)), as well as anthropometric measurements (weight, height) were gathered. The following IR-related parameters were calculated Homeostatic Model Assesment – Insulin Resistance (HOMA-IR) and TG/HDL ratio. Results: Three-month vitamin D supplementation increased the mean vitamin D concentration to generally accepted physiological level independently of T2DM presence. Importantly, vitamin D exposure decreased the level of oxidative DNA damage in lymphocytes of patients of intervention group. Among studied metabolic parameters, vitamin D markedly increased HDL level, decreased HOMA-IR, TG/HDL ratio. Furthermore, we found that HbA1c percentage diminished about 0.5% in T2DM patients supplemented with vitamin D. Conclusion: The current study demonstrated that daily 2000I U intake of vitamin D for three months decreased the level of oxidative DNA damage, a marker of oxidative stress, independently on T2DM presence. Furthermore, vitamin D reduced metabolic parameters connected with IR and improved glucose and lipid metabolism. Therefore, our results support the assertion that vitamin D, by reducing oxidative stress and improving of metabolic profile, may decrease IR and related diseases.
Y Chromosome, Hypertension and Cardiovascular Disease: Is Inflammation the Answer?
Authors: Khan Shanzana I. , Andrews Karen L. , Jennings Garry L. , Sampson Amanda K. , Chin-Dusting Jaye P. F.
Published: June 2019

It is now becomingly increasingly evident that the functions of the mammalian Y chromosome are not circumscribed to the induction of male sex. While animal studies have shown variations in the Y are strongly accountable for blood pressure (BP), this is yet to be confirmed in humans. We have recently shown modulation of adaptive immunity to be a significant mechanism underpinning Y-chromosome-dependent differences in BP in consomic strains. This is paralleled by studies in man showing Y chromosome haplogroup is a significant predictor for coronary artery disease through influencing pathways of immunity. Furthermore, recent studies in mice and humans have shown that Y chromosome lineage determines susceptibility to autoimmune disease. Here we review the evidence in animals and humans that Y chromosome lineage influences hypertension and cardiovascular disease risk, with a novel focus on pathways of immunity as a significant pathway involved.
Correction: Warzecha, Z. et al. Protective Effect of Pretreatment with Acenocoumarol in Cerulein-Induced Acute Pancreatitis. Int. J. Mol. Sci. 2016, 17, 1709
Authors: Warzecha Zygmunt , Sendur Paweł , Ceranowicz Piotr , Dembiński Marcin , Cieszkowski Jakub , Kuśnierz-Cabala Beata , Olszanecki Rafał , Tomaszewska Romana , Ambroży Tadeusz , Dembiński Artur
Published: June 2019

We would like to submit the correction to our published paper [...]
What Message Characteristics Make Social Engineering Successful on Facebook: The Role of Central Route, Peripheral Route, and Perceived Risk
Authors: Algarni Abdullah
Published: June 2019

Past research suggests that the human ability to detect social engineering deception is very limited, and it is even more limited in the virtual environment of social networking sites (SNS) such as Facebook. At the organizational level, research suggests that social engineers could succeed even among those organizations that identify themselves as being aware of social engineering techniques. This may be partly due to the complexity of human behaviors in failing to recognize social engineering tricks in SNSs. Due to the vital role that persuasion and perception play on users’ decision to accept or reject social engineering tricks, this paper aims to investigate the impact of message characteristics on users’ susceptibility to social engineering victimization on Facebook. In doing so, we investigate the role of the central route of persuasion, peripheral route of persuasion, and perceived risk on susceptibility to social engineering on Facebook. In addition, we investigate the mediation effects between the explored factors, and whether there is any relationship between the effectiveness of them and users’ demographics.
Lymphocytopenia as a Predictor of Mortality in Patients with ICU-Acquired Pneumonia
Authors: Ceccato Adrian , Panagiotarakou Meropi , Ranzani Otavio T. , Martin-Fernandez Marta , Almansa-Mora Raquel , Gabarrus Albert , Bueno Leticia , Cilloniz Catia , Liapikou Adamantia , Ferrer Miquel , Bermejo-Martin Jesus F. , Torres Antoni
Published: June 2019

Background: Intensive care unit-acquired pneumonia (ICU-AP) is a severe complication in patients admitted to the ICU. Lymphocytopenia is a marker of poor prognosis in patients with community-acquired pneumonia, but its impact on ICU-AP prognosis is unknown. We aimed to evaluate whether lymphocytopenia is an independent risk factor for mortality in non-immunocompromised patients with ICU-AP. Methods: Prospective observational cohort study of patients from six ICUs of an 800-bed tertiary teaching hospital (2005 to 2016). Results: Of the 473 patients included, 277 (59%) had ventilator-associated pneumonia (VAP). Receiver operating characteristic (ROC) analysis of the lymphocyte counts at diagnosis showed that 595 cells/mm3 was the best cut-off for discriminating two groups of patients at risk: lymphocytopenic group (lymphocyte count <595 cells/mm3, 141 patients (30%)) and non-lymphocytopenic group (lymphocyte count ≥595 cells/mm3, 332 patients (70%)). Patients with lymphocytopenia presented more comorbidities and a higher sequential organ failure assessment (SOFA) score at the moment of pneumonia diagnosis. Also, 28-day mortality and 90-day mortality were higher in patients with lymphocytopenia (28-day: 38 (27%) versus 59 (18%), 90-day: 74 (53%) versus 111 (34%)). In the multivariable model, <595 cells/mm3 resulted to be an independent predictor for 90-day mortality (Hazard Ratio 1.41; 95% Confidence Interval 1.02 to 1.94). Conclusion: Lymphocytopenia is an independent predictor of 90-day mortality in non-immunocompromised patients with ICU-AP.
Deep Convolutional Neural Network-Based Positron Emission Tomography Analysis Predicts Esophageal Cancer Outcome
Authors: Yang Cheng-Kun , Yeh Joe Chao-Yuan , Yu Wei-Hsiang , Chien Ling-I. , Lin Ko-Han , Huang Wen-Sheng , Hsu Po-Kuei
Published: June 2019

In esophageal cancer, few prediction tools can be confidently used in current clinical practice. We developed a deep convolutional neural network (CNN) with 798 positron emission tomography (PET) scans of esophageal squamous cell carcinoma and 309 PET scans of stage I lung cancer. In the first stage, we pretrained a 3D-CNN with all PET scans for a task to classify the scans into esophageal cancer or lung cancer. Overall, 548 of 798 PET scans of esophageal cancer patients were included in the second stage with an aim to classify patients who expired within or survived more than one year after diagnosis. The area under the receiver operating characteristic curve (AUC) was used to evaluate model performance. In the pretrain model, the deep CNN attained an AUC of 0.738 in identifying patients who expired within one year after diagnosis. In the survival analysis, patients who were predicted to be expired but were alive at one year after diagnosis had a 5-year survival rate of 32.6%, which was significantly worse than the 5-year survival rate of the patients who were predicted to survive and were alive at one year after diagnosis (50.5%, p < 0.001). These results suggest that the prediction model could identify tumors with more aggressive behavior. In the multivariable analysis, the prediction result remained an independent prognostic factor (hazard ratio: 2.830; 95% confidence interval: 2.252–3.555, p < 0.001). We conclude that a 3D-CNN can be trained with PET image datasets to predict esophageal cancer outcome with acceptable accuracy.
Secretory Acid Sphingomyelinase in the Serum of Medicated Patients Predicts the Prospective Course of Depression
Authors: Mühle Christiane , Wagner Claudia , Färber Katharina , Richter-Schmidinger Tanja , Gulbins Erich , Lenz Bernd , Kornhuber Johannes
Published: June 2019

Major depressive disorder (MDD) is a highly prevalent and devastating psychiatric illness with strong individual and societal burdens. However, biomarkers to improve the limited preventive and therapeutic approaches are scarce. Multilevel evidence suggests that the pathophysiological involvement of sphingolipids particularly increases the levels of ceramides and the ceramide hydrolyzing enzyme, acid sphingomyelinase. The activity of secretory acid sphingomyelinase (S-ASM) and routine blood parameters were determined in the serum of patients with current (unmedicated n = 63, medicated n = 66) and remitted (n = 39) MDD and healthy subjects (n = 61). Depression severity and anxiety and their 3-weeks prospective course of treatment were assessed by psychometric inventories. S-ASM activity was not different between the four groups, did not decrease during treatment, and was not lower in individuals taking medication that functionally inhibited ASM. However, S-ASM correlated positively with depression severity only in remitted patients. High enzyme activity at inclusion predicted milder clinician-evaluated and self-rated depression severity (HAM-D, MADRS, BDI-II) and state anxiety at follow-up, and was related to stronger improvement in these scores in medicated patients. S-ASM was strongly and contrariwise associated with serum lipids in unmedicated and medicated females. These findings contribute to a better understanding of the pathomechanisms underlying depression and the development of clinical strategies and biomarkers.
Overexpression of IL-10 Enhances the Efficacy of Human Umbilical-Cord-Derived Mesenchymal Stromal Cells in E. coli Pneumosepsis
Authors: Jerkic Mirjana , Masterson Claire , Ormesher Lindsay , Gagnon Stéphane , Goyal Sakshi , Rabani Razieh , Otulakowski Gail , Zhang Haibo , Kavanagh Brian P. , Laffey John G.
Published: June 2019

Enhancing the immunomodulatory effects of mesenchymal stromal cells (MSCs) may increase their effects in sepsis. We tested the potential for overexpression of Interleukin-10 (IL-10) in human umbilical cord (UC) MSCs to increase MSC efficacy in Escherichia coli (E. coli) pneumosepsis and to enhance human macrophage function. Pneumonia was induced in rats by intratracheal instillation of E. coli ((2.0–3.0) × 109 Colony forming units (CFU)/kg). One hour later, animals were randomized to receive (a) vehicle; (b) naïve UC-MSCs; or (c) IL-10 overexpressing UC-MSCs (1 × 107 cells/kg). Lung injury severity, cellular infiltration, and E. coli colony counts were assessed after 48 h. The effects and mechanisms of action of IL-10 UC-MSCs on macrophage function in septic rodents and in humans were subsequently assessed. Survival increased with IL-10 (9/11 (82%)) and naïve (11/12 (91%)) UC-MSCs compared to vehicle (9/15 (60%, p = 0.03). IL-10 UC-MSCs—but not naïve UC-MSCs—significantly decreased the alveolar arterial gradient (455 ± 93 and 520 ± 81, mmHg, respectively) compared to that of vehicle animals (544 ± 52, p = 0.02). Lung tissue bacterial counts were significantly increased in vehicle- and naïve-UC-MSC-treated animals but were not different from sham animals in those treated with IL-10 overexpressing UC-MSCs. IL-10 (but not naïve) UC-MSCs decreased alveolar neutrophils and increased alveolar macrophage percentages compared to vehicle. IL-10 UC-MSCs decreased structural lung injury compared to naïve UC-MSC or vehicle therapy. Alveolar macrophages from IL-10-UC-MSC-treated rats and from human volunteers demonstrated enhanced phagocytic capacity. This was mediated via increased macrophage hemeoxygenase-1, an effect blocked by prostaglandin E2 and lipoxygenase A4 blockade. IL-10 overexpression in UC-MSCs enhanced their effects in E. coli pneumosepsis and increased macrophage function. IL-10 UC-MSCs similarly enhanced human macrophage function, illustrating their therapeutic potential for infection-induced acute respiratory distress syndrome (ARDS).
Comparative Study on Fuel Gas Supply Systems for LNG Bunkering Using Carbon Dioxide and Glycol Water
Authors: Choi Jungho , Park Eun-Young
Published: June 2019

Liquified natural gas (LNG) fuel has received significant attention as an affordable and highly efficient fuel option due to strengthened regulations on the sulfur content of bunker oil put in place by the International Maritime Organization. The main component of the LNG fuel gas supply system (FGSS) is the heat exchanger that provides adequate gas temperatures and pressures required by the engine, which also has a large machinery volume compared with other equipment. Due to the volume limitation, most FGSS have been applied to new shipbuilding only. To reduce the volume of the FGSS, CO2 was considered to serve as the replacement heat medium for conventionally used glycol water during LNG gasification. The specific power consumption (SPC) in the CO2 and glycol water system was optimized using the Aspen HYSYS thermodynamic modeler toward adjusting the temperature and pressure, and the resulting sizes were compared. This study demonstrated that the CO2 heat medium resulted in a 14% improvement in efficiency and a 7% reduction in heat exchanger size concluding that it was the most advantageous heat medium for the LNG regasification.