bluuebunny/crossref_metadata_2025 huggingface dataset:
ID: 1019 Efficiency of the combination of modified acupuncture and motor relearning method in post-stroke patients
Authors: Man Bui Pham Minh , Dieu Thuong Trinh Thi
Published: September 2017

Background and Objectives: The combination of modern medicine and traditional medicine in the rehabilitation of motor deficit after stroke has shown interesting results. Many studies on modified acupuncture, a combination of modern and traditional techniques, have proven its effectiveness in motor rehabilitation in post-stroke patients. Furthermore, many studies have elucidated the effectiveness of the motor relearning method in the treatment of post-stroke paralysis. Therefore, this study aims to determine whether the combination of modified acupuncture and motor relearning method can improve the treatment results.  Method: Multi-centered randomized controlled trial (Traditional Medicine Hospital of Ho Chi Minh City, General Hospital of Soc Trang Province, and People Military Hospital of Soc Trang Province) from July 2014 to July 2015. 66 post-stroke patients were divided into two groups: a control group received modified acupuncture combined with Bobath method and a trial group received modified acupuncture combined with motor relearning method. After six weeks, patients were evaluated according to the Barthel score, 10-hole test, and 10-meter-walk test.  Results: The trial group showed better results than the control group did. There was a significant difference between two groups in Barthel score and 10-meter-walk test, but not in the 10-hole test. After treatment, 77.42% patients of the trial group showed improvement while only there are 51.61% in the other group (P < 0.05).  Conclusion: The combination of modified acupuncture and motor relearning method is more effective than the combination of modified acupuncture and Bobath method in the rehabilitation of motor deficit after stroke.
ID: 1020 Direct reprogramming of fibroblasts into endothelial progenitor cells by defined factors
Authors: Truong Mai Thi-Hoang , Huynh Oanh Thuy , Phan Liem Hieu , Pham Phuc Van
Published: September 2017

Background: Endothelial progenitor cells (EPCs) are important progenitor cells in vasculogenesis as well as in tissue engineering. However, few EPCs can be isolated from bone marrow, peripheral blood and umbilical cord blood. Moreover, their in vitro proliferation potential is also limited. Therefore, this study aimed to produce EPCs from direct reprogramming of fibroblasts by transduction with certain specific factors.

 Methods: Human fibroblasts were collected from human skin by published protocols. The cells were transduced with 2 viral vectors containing 5 factors, including Oct3/4, Sox2, Klf4, c-Myc (plasmid 1), and VEGFR2 (plasmid 2). Transduced cells were treated with endothelial cell medium for 21 days. The cells were analyzed for expression of Oct3/4, Sox3, Klf4, c-Myc and VEGFR2 at day 5, and for EPC phenotype at day 21.

 Results: The results showed that after 5 days of transduction, fibroblasts acquired partial pluripotency. After 21 days of transduction and culture in endothelial cell medium, the cells exhibited endothelial markers (e.g. CD31 and VEGFR2) and formed blood vessel-like capillaries.

 Conclusion: Our findings suggest another strategy for direct reprogramming of fibroblasts into EPCs.
ID: 1021 Experimental reprogramming of murine embryonic fibroblasts towards induced pluripotent stem cells using a single polycistronic vector
Authors: Huynh Oanh Thuy , Truong Mai Thi-Hoang , Pham Phuc Van
Published: September 2017

Background: Embryonic stem cells are pluripotent, thus capable of differentiating into all types of cells derived from the three germ layers. However, the application of embryonic stem cells (ESCs) for preclinical and clinical studies is difficult due to ethical concerns. Induced pluripotent stem cells (iPSCs) are derived from differentiation and have many ESC characteristics. The study herein examines the production of iPSCs from reprogramming of mouse embryonic fibroblasts (MEFs) via transduction with defined factors.

 Methods: MEFs were collected from mouse embryos via a previously published protocol. The cells were transduced with a single polycistronic viral vector encoding mouse cDNAs of Oct3/4, Sox2, Klf4 and c-Myc. Transduced cells were treated and sub- cultured with ESC medium. The cells were evaluated as iPSCs with specific morphology, and expression SSEA-1, Oct3/4, Sox2 and Nanog. In addition, they were also evaluated for pluripotency by assessing alkaline phosphatase (AP) activity and in vivo teratoma formation.

 Results: Under the reprogrammed conditions, the transduced cells displayed a change in morphology, forming ESC-like clusters. These cell clusters strongly expressed pluripotent markers as well as ESC-specific genes. Furthermore, the colonies exhibited higher AP activity and formed teratomas when injected into the murine testis.

 Conclusion: The study herein suggests that MEFs can be reprogrammed into iPSCs using a polycistronic viral vector encoding mouse cDNAs for Oct3/4, Sox2, Klf4 and c- Myc
ID: 1028 Comparison of HER2 status in gastric adenocarcinoma patients between gastrectomy specimens and matched endoscopic specimens
Authors: Hung Cuong Pham , Nu Hoang Yen Tran
Published: September 2017

Background: To evaluate the concordance between HER2 status in both surgical specimens and matched endoscopic specimens of gastric adenocarcinoma patients.

Methods: Records of 52 patients with gastric adenocarcinoma diagnosed by upper GI endoscopy, treated by gastrectomy and Her2 status assessed (by immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH)) from June 2014 through July 2016 in HCMC Oncology Hospital were studied and presented in case series. Her2-positive status was defined by a score 3+ in IHC, or 2+ with a positive FISH test.

Results: + Her2-positive status was identified in 9.6%.

+ The concordance of Her2 status between endoscopic biopsy and surgical specimens was 98% (Kappa=0.879).

Conclusion: + There was a very high concordance between the Her2 status results performed on surgical specimens and matched endoscopic specimens.

+ Her2 status assessed on endoscopic biopsy specimens could be reliable for treatment decisions using anti-Her2 agents in patients with advanced gastric carcinoma
ID: 1030 Observing an expression of TNF-α, IL10 and HBEGF on peripheral blood mononuclear cells (CD14+) of a guttate psoriatic patient at Ho Chi Minh City
Authors: Viet Nguyen Hong , Hoa Pham Cuc
Published: September 2017

Psoriasis is a chronic autoimmune disease in dermatology. The experiment collected peripheral blood samples from cohorts. These samples were treated and sorted mononuclear leukocytes with LPS receptors or CD14. Then, they were stimulated by a lysis of Streptococcus pneumonia in 24 hours. These cells were harvested in 0, 1, 24, 48, 72,120 (day 5) and 168 (day 7) hours and were isolated total mRNA. All mRNA samples were converted into the cDNA samples which were utilised to indirectly evaluate the cytokine expression of TNF-α, IL10 and Heparin-binding EGF-like growth factor (HBEGF) on the mononuclear cells (CD14+) in the period shown. Regarding result of the psoriatic patient, three cytokines abnormally prolonged after the 24 hours induction. The signals of TNF-α, IL10 and HBEGF expanded throughout 48 hours,5 days and 7 days, respectively. In addition, a band (627bp) was obtained in from PCR products of IL10. Furthermore, sequencing the 627bp band shows that it was homologous to a fragment on chromosome 2. Meanwhile, the results of those cytokines on a cohort without non-psoriasis were slightly exposed in around 48 hours. In conclusion, the mononuclear cells (CD14+) in the cohort with guttate psoriasis is highly more sensitive with a lysis of Streptococcus pneumonia than the non-psoriatic cohort in TNF-α expression, and  IL10 and HBEGF of the psoriatic one are also simultaneous and extended during the inflammation and wound healing. Furthermore, the 620bp band might relate to edit progressing pre-mRNA IL10 in the guttate psoriasis.
ID: 1039 The antidepressant fluoxetine inhibits adenylate cyclase stimulation by FSH or Forskolin in the COV434 human ovarian granulosa tumor cell line
Authors: Nguyen Thi Mong Diep , Klett Danièle , Vo Minh Thu , Combarnous Yves
Published: September 2017

Fluoxetine (Prozac), a selective Serotonin Reuptake Inhibitor antidepressant, exhibits other mechanisms of action in various cell types and has been shown to induce cell death in cancer cells, paving the way for its potential use in cancer therapy. The ovary is a complex endocrine organ responsible for steroidogenesis and folliculogenesis, and human granulosa cells are essential for scientific research to improve the understanding of these two processes. However, little is known about fundamental signaling pathways in human granulosa cells. In this study, we investigated the dynamics of intracellular cyclic adenosine monophosphate AMP, a conserved signaling messenger that can regulate virtually every physiological process. We show that incubating COV434 human ovarian granulosa cells with fluoxetine induces a decrease in intracellular cAMP response to Follicle-stimulating hormone (FSH) and forskolin (FSK). In order to study the intracellular cAMP kinetic responses of COV434 cells to FSH or FSK, we used COV434 cells transiently expressing a chimeric cAMP-responsive luciferase so that real-time variations of intracellular cAMP concentration could be monitored, by using oxiluciferin luminescence produced from catalyzed luciferin oxidation. Our data show that fluoxetine induces an increase in the extracellular Ca2+ entry and reduces ATP concentration as well as cell viability. Targeting these signaling pathways with fluoxetine could permit to get better knowledge in the molecular mechanisms involved in ovarian follicular development
ID: 1043 Study value of 12-lead ECG for the left free wall of accessory pathways diagnosing in patients with typical Wolff-Parkinson-White Syndrome
Authors: Si Chu Dung , Khanh Pham Quoc , Dong Tran Van
Published: September 2017

Objectives: This study was designed characteristics of 12-lead Electrocardiogram (ECG) for the left free wall lateral of accessory pathway (AP) localization in the typical WPW syndrome to develop a new algorithm ECG for localizing APs, and to test the accuracy of the algorithm prospectively.

Methods: We studied 129 patients, in 84 patients with typical WPW syndrome have a single anterograde with the localization of APs identified by successful radiofrequency catheter ablation (RCFA) to develop a new ECG algorithm for the left free wall of localizing APs. Then this algorithm was tested prospectively in 45 patients were compared with the location of AP’s successful ablation by RCFA.

 Results: We found that the 12 lead ECG parameters in typical WPW syndrome such as delta wave polarity in V1, R/S ratio in V1, the transition of the QRS complex, delta wave polarity in inferior leads in diagnosis for the localization of APs by with high accuracy predicted from 74,5% to 100%, and for development of a new ECG algorithm. Then the following 45 patients were prospectively evaluated by the new derived algorithm for the left free wall pathways with high sensitivity and specificity from 75% to 100%.

Conclusion: 12-lead ECG parameters in typical WPW syndrome closely related to left free wall of APs localization, in order to develop the new ECG algorithm by parameters as above; and can be used to a new ECG algorithm in predicting the location APs with high accuracy.
ID: 1044 DNA origami nanobot for sensitive drug delivery chemotherapy
Authors: Luu Minh Tri , Wickham Shelley , Abbas Ali
Published: September 2017

The cutting-edge technology of constructing nanoscale objects using DNA origami has opened new directions for drug delivery in cancer chemotherapy research [1, 2]. This project aims to develop a novel DNA origami nanobot for drug delivery, with high selectivity and specificity for chemotherapy. It is important to be able to control the rate of drug release to maintain the concentration of chemotherapeutic agents at the desirable set-point [3]. This control can be achieved through various activation methods, similar to those used in liposome drug delivery systems, e.g. magnetism, radiation, ultrasound, heating etc. [4]. These stimuli can deliver specific types of energy (e.g. thermal), which can then activate a pre-designed nanobot- topology variation. For example, thermal energy can cause local DNA strands to melt and partially distort some local regions of the DNA topology, releasing drug molecules. One mechanism to activate the drug release is via radio frequency (RF) electromagnetic wave induced heating of gold nanoparticles [6]. A prototype nanobot will be developed and tested for heat-triggered nanobot switching between open and closed configurations. It is hypothesized that upon RF heating, the gold nanoparticles will concentrate the heat and cause the local DNA strands to melt, leading to the open configuration, without melting the rest of the nanobot structure. Heating time and power will be tuned to regulate the drug release rate. This work will develop an effective process control strategy for enhanced performance of nanoscale drug delivery systems.
ID: 1045 Oxygen carrier containing microparticles for prolonged cell survival and effective bone regeneration
Authors: Gim So Yeong , Kim Ho Yong , Byun June Ho , Lee Jin Ho , Oh Se Heang
Published: September 2017

The organ transplantation is considered as an effective therapeutic method for organ failure. However, the shortage of donor organ and the immunological rejection are still remained as a big huddle in clinical fields. As an alternative, the tissue-engineering approach for functional organ replacement is extensively studied. The 3D porous scaffolds which guide growth and differentiation of cells are widely utilized as a vital factor with autologotic cells and growth factors for tissue engineering. However, cell necrosis in the center of 3D cell scaffold, due to the insufficient oxygen diffusion is considered as critical limitation for the formation of 3D tissues. Recently, various oxygen delivering/releasing materials have been studied to overcome this limitation. In this study, we developed a perfluorooctane (PFO)-loaded hollow microparticles (PFO-HP) which can allow sustained oxygen delivery for sufficient period, and thus provide appropriate environment for cell survival even in hypoxia. Cell (human periosteum-derived cell, hPDC) behaviors (proliferation, apoptosis, protein synthesis, etc.) in hypoxia (1% O2 and 5% CO2) were evaluated for 28 days. And osteogenic differentiation of cell [alkaline phosphatase (ALP activity), calcium deposition, immunocytochemistry, and RT-PCR (RUNX2, collagen type Ⅰ, osteocalcin, osteonectin)] after 10 days exposure in hypoxic condition was also investigated. New bone formation (histology, CT) of the cell-loaded PFO-HP and PBS-HP was also investigated using a miniature pig (mandibular defect model).
ID: 1048 A novel nonsense mutation in the CYP21A2 gene of a Vietnamese patient with congenital adrenal hyperplasia
Authors: Dung Vu Chi , Nguyen Ngoc Lan , Nguyen Huy Hoang , Nguyen Thi Kim Lien , Tran Thinh Huy , Ta Thanh Van , Tran Van Khanh
Published: September 2017

Inactivating mutations in the CYP21A2 gene which encodes the protein involved in steroid synthesis have been reported in the patients with congenital adrenal hyperplasia (CAH). An infant who diagnosed with the severe phenotype of CAH such as increasing testicular volume, elevating of 17-hydroxyprogesteron, testosterone and progesterone and his family were subjected for genetic studies. Initially, we used PCR and direct sequencing to screen mutations in the CYP21 gene in the proband and his family. We identified a novel nonsense mutation c.374C>G predicts a substitution of serine for a stop codon at codon 125 (p.S125*) within exon 3 in the proband. However, the inheritance pattern of the mutation was not consistent with disease causation because of a heterozygous mutation carrier in father and sibling, wild-type alleles in mother but mutant alleles in proband. This inspired us to find deletions of exon using multiplex ligation-dependent probe amplification (MLPA) assay. In the profiles of MLPA electropherogram, the proband had a large deletion in exon 3, but his mother did not have. It means that the proband inherited a normal allele from his mother and a mutant allele from his father, but the deletion of a normal allele occurred in the proband. Therefore, mutation c.374C>G (p.S125*) in exon 3 in the proband is considered as a heterozygous deletion mutation. In addition, a large deletion in exon 1 in the maternal allele in the proband is observed. Taking together, the proband carried a nonsense mutation accompanied with two deletions in exon 1 and exon 3 in the CYP21A2 gene affect the CAH phenotype severity. These mutations also expand the CYP21A2 mutation spectrum in CAH disorder. This case also highlights the need of caution when interpreting results of molecular genetics and biochemical testing during genetic counseling.
ID: 1052 Influence of Graphene oxide and Collagen-based biomaterials modulate the characteristic of Human Amnion-Derived Mesenchymal Stem Cells
Authors: Akhir Haselamirrah Mohd , Lin Teoh Peik
Published: September 2017

Biomaterials serve as a fundamental component of tissue engineering. They are designed to imitate the native extracellular matrix to support cell growth. Tissue regeneration has the potential to achieve this by combining biomaterials design with cells from the body. The objectives of this study are to examine how biomaterials regulate the expression of genes related to proliferation, pluripotency and differentiation of amnion-derived mesenchymal stem cells (AM-MSCs). AM-MSCs co-cultured with collagen and GO showed comparable proliferation rate as compared to controls. Induction of osteogenic and adipogenic differentiation media on AM-MSCs was not affected by these biomaterials although collagen was found to promote adipogenesis in normal medium. The RT-PCR results showed that cells co-cultured with collagen downregulated the expression of pluripotency genes, stem cell surface markers while increased the expression of adipogenic genes. In contrast to collagen, GO increased the expression of pluripotency genes. In conclusion, these biomaterials differentially modulate the gene expression of surface markers and several differentiation genes without influencing cell viability.
ID: 1054 Comparison between fresh and cryopreserved Human Amnion Mesenchymal Stem Cells (HAMCs) in terms of serial passaging, morphology and differentiation potential during long term culture
Authors: Gumpil Sandra Lisa , Ampon Kamaruzaman , Lasimbang Helen , Fatimah Simat Siti , Kumar S.Vijay
Published: September 2017

The therapeutic efficiency of Human Amnion Mesenchymal stem cells (HAMCs) is significantly promising. However, its sustainability in long term sub-cultivation has yet to be identified, especially post-cryopreservation. There are concerns whether stem cells which are cryopreserved will be able to retain its functions and differentiation potential efficiently. The aim of this study is to determine the effects of cryopreservation on the serial passaging, morphology and differentiation potential of HAMCs culture post-cryopreservation compared to fresh HAMCs culture. HAMCs was isolated through serial enzymatic digestion of the amnion membrane of human term placenta after delivery. Fresh HAMCs cultures were sub-cultivated until passage 15 while cryopreserved HAMCs samples was preserved at passage 2 of the cell culture and revived after 3 months of cryopreservation period. Through microscopic observation, the cryopreserved HAMCs started to flatten and become larger in size, losing it fibroblastic feature as early as passage 6. The enlarged and flatten morphological features of the HAMCs indicate that the cells began to lose its proliferative activity at this point. In comparison, the HAMCs normally began to enlarge when reaching passage 15 in non-cryopreserved HAMCs cultures. Fresh HAMCs cultures were able to be sustained up to 15 passages while cryopreserved HAMCs were not able to survive pass 10 passages of cell culture. Furthermore, it was observed that both fresh and cryopreserved HAMCs were able to retain its differentiation potential through osteogenesis and adipogenesis medium induction. This was experimentally visualized through positive staining of the calcium composite and lipid droplet in the induced HAMCs culture using Von Kossa stain solution and Oil Red-O stain solution, respectively. Despite retaining it differentiation potential, cryopreserved HAMCs were only able to survive a few passages after being revived. The result suggests that fresh HAMCs is a more suitable candidate to be used in cellular therapy and various clinical application as it were able to retain all its function in long term culture.
ID: 1063 Isolation and characterization of female germline stem cell derived from porcine ovary
Authors: Hoang Nguyen Huy , Tran Nguyen Thi Bao , Thuan Nguyen Van , Thuy Bui Hong
Published: September 2017

One of the most significant finding in stem cell area in the early 21st century is the founding of female germline stem cells (FGSCs). Establishment of FGSCs allowed new possibilities for the use of them in biotechnology and medicine. Hence, the purpose of this study was to establish, characterize the porcine female germline stem cells (pFGSCs) from porcine ovary. The result revealed the success in establishing pFGSCs from ovarian tissue. Most of the pFGSCs were round shape after in vitro culture, forming groups of cells that cluster around the ovarian cells colonies. Immunofluorescent analysis of pFGSCs showed that these cells expressed germ cell and stem cell markers such as: Vasa, Stella, c-kit and Oct4. After several weeks in in vitro culture, pFGSCs increased in number without the loss of proliferative potential. Our results suggested that pFGSCs isolated from adult mammalian ovary, under appropriate conditions, could undergo proliferation.
ID: 1064 Effects of FSH, cumulus cell morphology and follicular fluid from different follicular sizes on the in vitro maturation of bovine oocytes
Authors: Linh Nguyen Hoang-Kieu , Doan Phung Ngoc Minh , Duy Pham Truong , Thuy Bui Hong , Thuan Nguyen Van
Published: September 2017

The quality of mature oocyte plays a vital role in assisted reproductive technology, as well as animal cloning. Therefore, optimization of the in vitro maturation procedure for oocytes has long been of interests for researchers in the fields of reproduction. In this study, we investigated the effect of different supplement culture factors on in vitro maturation of bovine oocytes such as follicular-stimulating hormone (FSH) (experiment 1), different layers of cumulus cells (CCs) (experiment 2), and follicular fluid (FF) collected from different follicle sizes (experiment 3). With result from experiment 1, bovine oocytes cultured in in vitro maturation (IVM) medium supplemented with FSH reached to higher maturation rate than cultured in the basic one (85.9% and 69.3% respectively). In addition, experiment 2 suggested that, the groups of 3-4 layers and 2-3 layers achieve higher rate of oocyte maturity than group of <1 layers (84.38%; 82.46%; 47.83% respectively). However, the result of experiment 3 show that FF collected from different follicle size did not affect to the maturation rate. In conclusion, FSH and layers of CCs affect to the maturation of bovine oocytes.
ID: 1065 Establishment of parthenogenetic diploid embryonic stem cells in mice
Authors: Ngan Ho Thi-Kim , Thuan Nguyen Van , Bui Hong-Thuy
Published: September 2017

Parthenogenesis is a process in which zygotes are produced without sperm presence. Due to lack of paternal genes, parthenogenetic embryos cannot develop to full-term; however, these embryos show a great potential to generate histocompatible stem cells (parthenogenetic embryonic stem – pES cells) for transplantation. In this research, parthenogenetic activation in the mouse was carried out using strontium chloride (SrCl2) combined with cytochalasin B (CB). The rate of embryo development, blastocyst quality and expression of acetylation of histone H4 lysine 12 (H4K12Ac) were investigated, while parthenogenetic blastocysts were used to establish pES cells. The results showed that rate of in vitro blastulation of parthenogenetic embryos was lower than that of fertilized ones (45.1% vs 98.0%, respectively). In addition, blastocysts developed from parthenogenetic embryos also expressed lower quality, which was demonstrated by lower total cell number. Moreover, H4K12Ac expression significantly decreased in the inner cell mass (ICM) of parthenogenetic blastocysts compared to fertilized ones, indicating a possible reason for lower blastocyst quality. Following embryo collection and activation, two ES cell lines – fertilized (fES) and pES cell lines have been successfully established and maintained long term in vitro. To sum up, differences in blastocyst quality and H4K12Ac expression in ICM cells of blastocyst may contribute to aberrant developmental and embryonic stem cell formation in parthenogenetic embryos.
ID: 1067 The Effect of Oocyte-Cumuls Cell Complexes on Maturation Rates and Pronuclear Formation After Pathenogenetic Activation
Authors: Nhan Bui Thanh , Tram Nguyen Dang Bich , Doan Phung Ngoc Minh , Thuy Bui Hong , Thuan Nguyen Van
Published: September 2017

Cumulus cells are integral in oocyte growth, maturation, pronuclear formation as well as preimplantation development after fertilization. In this study, we investigated the effect of different cumulus morphology on maturation rates and pronuclear formation after parthenogenetic activation. Oocyte-cumulus cell complexes (OCCs) were classified into three grades: (A) over 3 layers, (B) 2–3 layers, (C) less than 1 layer or incomplete. Our results showed that group A and B achieved high maturation rates (84.4% and 82.5%) whereas group C were 45.7%. In a similar pattern, after parthenogenetic activation, the rate of pronuclear formation of group A and B were namely 65.5% and 52%, while that of group C was only 36.4%. Moreover, we also examined the impact of duration of in vitro maturation on the rate of nuclear formation after activation. We found that 25-hour would result in the highest rate (55.5%) while only 4.44% in group of 30-hour, which was possibly caused by the aging of oocytes. In conclusion, this study found that OCCs with more than 2 layers of cumulus cells could reach the high maturation rates, finally oocytes activated at 25 hours resulted in high rate of pronuclear formation in bovine.
ID: 1071 Establishing an early-stage femoral head necrosis model of rabbit using methylprednisolone and Complete Freund's Adjuvant
Authors: Phi Lan Thi , Dao Thuy Thi-Thanh , Nguyen Khanh Dinh-Van , Pham Phuc Van , Bui Khanh Hong-Thien , Vu Ngoc Bich
Published: September 2017

Background: Pathological animal models provide the foundation for developing new methods for treating. This research aims to establish a rabbit model of femoral head necrosis. Osteonecrosis of the femoral head (ONFH) was induced in rabbits by using methylpresnisolone combined with Complete Freund's Adjuvant (CFA). New Zealand White rabbits were divided into two groups. Group A (n=10) was given an intramuscular injection of 0.5 mg/kg (CFA) and 40mg/kg methylprednisolone (MPS). Group B (n=6) was received normal saline at the same location and same volume as those in Group A. The efficiency of ONFH rabbit model was assessed at 7 weeks after the last injection. Body weight was weighed. The histological structure of head femoral and bone were deteded by H&E staining. The empty lacuna was counted. Cartilage degeneration was evaluated using image analysis software. Blood vessel density was assessed after ink artery infusion. The cell cycle of bone marrow-derived mononuclear cells was analyzed by flow cytometry. The results showed that there was no difference in body weight change of rabbits between two groups. However, the bone morphology and cartilage surface of femoral head were abnormalities at group A. The percentage of empty osteocyte lacunae were significantly higher in Group A than Group B. Chondrocyte degeneration and fibrocartilage expression were observed at Group A. Compare to group B, Group A had less ink-stained blood vessels. Moreover, the fraction of bone marrow-derived mononuclear at S phase and G2/M phase of the cell cycle was significantly decreased in group A. Thus, CFA combined with MPS can  be used to establishing an early-stage femoral head necrosis model of rabbit.
ID: 1072 Increasing dose of intravenous human adipose-derived stem cells improves the pancreatic function of diabetic mice
Authors: Dang Loan Thi-Tung , Bui Anh Nguyen Tu , Nguyen Cong Le Thanh , Truong Nhat Chau , Bui Anh Thi Van , Phan Ngoc Kim , Truong Kiet Dinh , Pham Phuc Van
Published: September 2017

Mesenchymal tem cell transplantation is a novel treatment for diabetes mellitus, especially type 1 diabetes. Many publications have proved the effect of MSC therapy on reducing blood glucose and improving insulin production in type 1 diabetic animal as well as in clinic trials. However, there is no conclusion that how many cells are effective for diabetes. Thus, this study has investigated that different adipose-derived MSC doses affected glucose metabolism in diabetic mice. STZ-induced diabetic mice were intravenous transfused with human MSCs with dose either 106 (treated group A) or 2x106 cells/mouse (treated group B). Both treated and untreated mice were monitored the blood glucose levels, glucose and insulin tolerance test, pancreatic structure change and insulin production every week until 56 days after transplantation. The results showed that the higher dose of MSC could reduce death rate (66% vs. 0% death in group A vs. group B after 56 days of treatment, respectively) and remarkably lower blood glucose levels while the mice treated with 1x106 cells/dose remaining hyperglycemia. Moreover, the glucose tolerance and insulin tolerance as well as insulin production were improved in group B at 28 days after transplantation. The histochemical imaging further demonstrated the decrease of inflammatory cells in the islets and the restoration of pancreatic structures in higher-dose-MSCs-treated mice. Thus, the dose 2x106 cells of MSCs may be an effective strategy for diabetes mellitus concerning hyperglycemia, impaired glucose metabolism and islet destruction.
ID: 1075 Concentration and κ/λ ratio of serum free light chain in multiple myeloma patients at Cho Ray Hospital
Authors: Phan Hoang Cong , Phan Thang Thanh , Ho Toan Trong , Tran Thu Bich , MCB Thanh Thanh , Nguyen Son Truong
Published: September 2017

Introduction: multiple myeloma (MM) is a B cell malignancy which characterized by accumulation of plasmocyte in bone marrow, immunoglobulins and free light chain in serum (sFLC). According to IMMWG (International multiple myeloma working group), sFLC is a criterion in diagnosis of MM. In this study, we investigate the concentration and κ/λ ratio of sFLC in newly diagnosed MM patients at Cho Ray hospital. Methods: cross-sectional study. Serum samples of 36 newly diagnosed MM patients were analyzed by Binding Site Freelite method to measure the amount of κ and λ sFLC.

 

Results: the median age of 36 MM patients was 61 yrs (41 – 88 yrs), with the male/female ratio was 1.2/1. MM stage III, stage II and stage I accounting for 63.9% (23/36), 27.8% (10/36), and 8.3% (3/36), respectively. Clonal IgG, IgA and IgE MM accounting for 61.1% (22/36), 30.6% (11/36), and 8.3% (3/36), respectively. We reported the median of plasmocyte in 36 cases was 35.4% (95%CI: 27.6 – 43.3). The median of concentration of κ sFLC, λ sFLC, and involved/uninvolved sFLC ratio were 1601.8 mg/L (95%CI: 104.9 – 3098.7), 900.1 mg/L (95%CI: 10.7 – 1789.5), and 191.0 (95%CI: 87.6 – 294.4), respectively. The abnormally high in sFLC concentration was reported in 97.2% MM cases (35/36), in which 55.6% cases (20/36) had increased κ sFLC, 19.4% cases (7/36) had increased λ sFLC, and 22.2% cases (8/36) had increased both κ+λ sFLC concentration. Increased involved/uninvolved sFLC ratio was found in 94.4% MM cases (34/36), in which 36.1% cases (13/36) had sFLC ratio > 100, with all involved sFLC concentration > 100 mg/L. In 3 cases of MM with kidney failure, 2 cases had increased both κ+λ sFLC, 1 case had increased κ sFLC concentration; and all of 3 cases had involved sFLC level > 5300 mg/L with sFLC ratio > 100. No significant difference of sFLC concentration and sFLC ratio among gender, stage of disease, Ig clone, or plasmocyte percent in bone marrow of MM patients. Conclusions: due to the abnormally high of sFLC concentration and sFLC ratio in MM, it is necessary to monitor frequently these parameters during treatment to prevent the risk of kidney failure for patients.
ID: 1076 Establishing an artificial cartilage from rabbit bone marrow derived mesenchymal stem cell and polycaprolactone scaffold
Authors: Dao Thuy Thi Thanh , Phan Liem Hieu , Le Ha Thi Ngan , Phi Lan Thi , Pham Phuc Van , Vu Ngoc Bich
Published: September 2017

Nowaday, the regenerative medicine based on the use of biomaterial scaffold combine to stem cells was considered as a necessary treatment strategy for injury or diseases involving cartilage. In this study, we investigated the adherence, proliferation and chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells (rBM-MSCs) on biodegradable polycaprolactone scaffold (PCL). MSCs were isolated from bone marrow of rabbit. rBM-MSC (2x105cell/scaffold) were seeded on PCL and collagen-coated PCL (PCL/col) scaffolds. The cell adhesion and proliferation abilities on the PCL and PCL/col scaffolds were compared to each other to find the better using MTT assay. Then 3D cultured-cells were induced in chondrogenic differentiation medium. The change of scaffold surface structure was evaluated by scanning electron microscope (SEM). The accumulation of aggrecan protein was detected by Alcian Blue staining. The chondrogenic-related gene expression such as sox9, collagen type 1, collagen type 2, collagen type 10, aggrecan, runx2 was assessed by RT-PCR. The existence of artificial cartilage after transplantation of chondrocyte-seeded scaffold under rabbit skin was evaluated by staining histological sections with Alcian Blue. The results showed that rBM-MSCs attached and grown on PCL/col and PCL scaffolds. However, the adhesion efficient of rBM-MSC on PCL/col scaffold was better than PCL scaffolds. Therefore, PCL/col scaffold was used for next experiments. The morphology of rBM-MSCs on PLC/col scaffold were changed and the aggrecan accumulation increased after cultured in chondrogenic differentiation medium. The scaffold surface modification was detected with the presentation of collagen-like fiber structures. The expression of chondrogenesis-associated genes increased after 3, 7, 21, 28 days of chondrogenic induction, especially for collagen type II, collagen type I, aggrecan and collagen type X. Transplanted artificial cartilage was existed in rabbit for six weeks. The mature chondrocyte-like cells were detected and cartilage specific-proteoglycan accumulated more in differentiated cell-scaffold than scaffold seeded undifferentiated-cells. Thus artificial cartilage could be created when differentiating rBM-MSC into chondrocyte on collagen-coated PCL scaffold.
ID: 1083 Targeting the production of oncogenic miRNAs using synthetic small molecules
Authors: Tran Anh Thi-Phuong , Vo Duc Huy , Giorgio Audrey Di , Duca Maria
Published: September 2017

MicroRNAs (miRNAs or miRs) are a class of evolutionary conserved small non-coding RNAs that act as post-transcriptional regulators of gene expression. A wide number of studies has shown that the aberrant expression of miRNAs could be responsible for initiation and development of human cancers. Most of these deregulated miRNAs are overexpressed, thus being oncogenic. For these reasons, the inhibition of oncogenic miRNAs function or production would be a very promising approach for the development of new anticancer therapies [1].

The purpose of this work is the discovery of small-molecule drugs targeting the precursors of specific oncogenic miRNAs thus modulating their production. We have focused our attention on miRNA-372 and miRNA- 373 that are implicated in various cancers. For example, these two oncogenics (pre-miRNAs 372 and pre-miRNAs 373): two stem-loop structured RNAs that lead to mature miRNAs after cleavage by the enzyme Dicer. In the aim of inhibiting this biogenesis step and based on our previous works [2], we synthesized a novel series of RNA ligands composed of two different domains: (i) 2-deoxystreptamine (2-DOS) known as RNA-interacting group due to its role as a central scaffold of any known aminoglycosides [3] and (ii) aromatic or heteroaromatic groups that should improve affinity and selectivity of these ligands for the targeted RNAs. These two domains have been conjugated through carbamate, triazole and ether bonds in order to develop a simple and straightforward synthetic methodology and obtain a large number of diversified compounds.

 

We obtained a library of 16 compounds which will be evaluated in vitro in order to identify the strongest RNA ligands that could be able to inhibit the production of the targeted oncogenic miRNAs and eventually lead to the inhibition of cancer cells proliferation.
ID: 1085 Sodium citrate induces apoptosis in HepG2 cell lines
Authors: Nguyen Sinh Truong , Vo Phuc Hong , Nguyen Oanh Thi-Kieu , Do Nghia Minh , Pham Phuc Van
Published: September 2017

PURPOSES: Cancer cells were observed to increase glucose uptake and fermentation of glucose to lactate to to synthesis rapidly ATP for cell growth, survival and proliferation. Thus, inhibition of glycolysis might be useful in antitumor treatment. This phenomenon occurred even with fully functioning mitochondria, and known as Warberg effect. Sodium citrate, an inhibitor of Warberg effect, was reported to antiproliferate many cancer cells line. However, sodium citrate has not been studied in Hepatocellular Carcinoma cells line yet. Here we aimed to investigate the effect of sodium citrate in HepG2 cells line.

 

MATERIAL AND METHODS: HepG2 cell lines was treated with sodium citrate at different concentrations. Viable cells were determined by Alamar Blue. The apoptosis induced-cells was detected by Annexin V with FCM technique. Disintegrated nuclei and DNA fragmentation was analyzed. The activity of caspase-3 was also tested.

 

RESULTS: We observed that the IC50 value of sodium citrate on HepG2 is at 10mM. FCM analysis showed that sodium citrate induced apoptosis in HepG2 cell line in dose-dependent manner. At 10mM sodium citrate, the caspase-3/7 was observed to be activated in time-dependent manner. Sodium citrate also induced nuclei disintergated in HepG2. DNA fragmentation was observed when HepG2 cells were treated with 10mM sodium citrate.

 

CONCLUSIONS: We have shown that sodium citrate possesses the antiproliferative ability on HepG2 at IC50 10mM. Sodidum citrate induces apoptosis cells in hepatocellular carcinoma HepG2 by capases-3 activation. More investigation of glycolysis inhibition of sodium citrate on HepG2 should be performed in animals
ID: 1087 Effects of oocytes collection and electro-activation protocols on maturation and preimplantation development of parthenogenetic diploid porcine embryos
Authors: Thuy Le Thi Thu , Tu Nguyen Ba , Thuy Bui Hong , Thuan Nguyen Van
Published: September 2017

Advances in porcine embryos production have been impaired by the in vitro maturation (IVM) system and in vitro developments (IVD) are still not optimized. The present study was undertaken to determine the effects of different oocytes collection methods (aspiration and dissection) on oocytes maturation and combine with the different electro-activation protocols on in vitro pre-implantation development of parthenogenetic diploid porcine embryos. The results showed that dissection method was significantly higher both in quantity (87.23%, p <0.05) of matured oocytes and in the rate of oocytes with very good quality (53.00%) compared with aspiration method (62.00%, 30.22%; respectively). We further accessed the quality of matured oocytes competence via parthenogenetic embryo development. Using four electro-activation protocols, we found that the electro-activation condition had no effect on the development of embryos to the 2-cell, 4-cell and 8-cell stages. However, the oocytes activated by the 60V/cm amplitude of pulse group had less (41.33%; p <0.05) development at the morula stage than 90V/cm, 100V/cm and 150V/cm amplitude of pulse groups (73.99%, 67.62%, 72.55%; respectively). The embryos develop to blastocyst stage was highest in the group induced by 90V/cm electro pulse was 71.76% compared to 100V/cm, 150V/cm, and 60V/cm (62.86%, 62.05%, and 37.63%, p<0.001, respectively). We conclude that the combining of dissection method for porcine oocytes collection and electro-activation with the amplitude of pulses 90V/cm can enhance both the activation success and development competition in in vitro pre- implantation parthenogenetic diploid porcine embryos.
Stephen Freeman of Antigua and London
Authors: Sommers Susan Mitchell
Published: September 2017

Until recently, Stephen Freeman (1739–90) was known only as the most obscure of a group of quack doctors attacked by James Makittrick Adair in Medical Cautions for the Consideration of Invalids. Adair’s censure is severe, and sufficiently absurd to be dismissed as hyperbole. However, the current author’s recent rediscovery of an apparently singular survival of Freeman’s reply, Strictures on Adair’s Bath Medical Cautions, demonstrates that while Adair’s description of Freeman as an indentured blacksmith in Antigua who abandoned his family to be supported by the parish is unkind, it is not entirely inaccurate. Freeman’s pamphlet is doubly remarkable as a rare autobiography by a poor and unwilling emigrant to Antigua, and as a memoir of a quack seeking to refashion himself as a reputable physician. In Antigua and London, Freeman relied on strategies commonly used by the rising middling classes to improve financial and social status: He established advantageous family connections, pursued a prestigious career, and joined a variety of societies, including freemasonry, which he may have been introduced to in the West Indies. Strictures is preeminently a documented narrative of Freeman’s struggle to construct and defend his respectability. His, however, is a cautionary tale, and ultimately none of the strategies he employed brought him the regularity and respectability he sought.
Fraternity and Biography
Authors: Tyssens Jeffrey
Published: September 2017

For a considerable part of the postwar period, biography did not have a good press at all in historiography: in the 1950s and 1960s vanguard historians usually rejected the format as a typical example of an old fashioned way of writing history, i.e. merely event-related and focussed almost exclusively on great (white) men. Since the 1970s however, microhistory and cultural turn helping, the biographical perspective has gradually returned as a legitimate format of historical research and new reflexive approaches towards its objects, goals and practices have come to the fore. In the history of freemasonry and fraternalism, biographies have been written as well (be it far less in number where non-masonic fraternities are concerned), but epistemological debate about its merits and methods has remained largely absent. This article makes a concise overview of theoretical debates about biography in current historiography and points at innovative approaches of biography writing that can inspire its application to the fraternal field at large.
Freemason and Philanthropist
Authors: Vergauwen David
Published: September 2017

The philanthropic activities of Edouard Jonniaux are indicative of the ways freemasons in Brussels mobilized the musical potential of their lodges to compete with Catholic charities. Jonniaux considered it a masonic obligation for the musicians of the capital’s lodges to perform for the latter’s philanthropic endeavours. As charity concerts were frequently being performed in churches, the Brussels masons decided to open up their lodge rooms for similar concerts and when this venue proved to be too small, they happily used the capital’s royal theatre, i.e. the national opera house where they had privileged connections. Especially during the 1860s, Brussels freemasonry was actively recruiting musicians, not the amateurs, but the professional ones. In order to integrate those talented musicians into the lodges, Jonniaux personally watched over their rights and supported free membership for musicians. All this resulted in many concerts that often served as a propaganda tool for the liberal and anticlerical cause.
Determination of soil erosion index for surface soils of landfill covers
Authors: Kumar Yamsani Sudheer , Kumar Sunil , Sekharan Sreedeep , Ranjan Rakesh Ravi
Published: September 2017

A multilayered cover (MLC) system is provided over a non-operational landfill for isolating the waste from the atmosphere. The integrity of the MLC is dependent on the stability of the surface layer. The surface layer in the MLC is a typical case of soil–atmosphere interaction due to its exposure to variations in temperature and rainfall with time. Surface erosion is identified as one of the factors that lead to the failure of the MLC. There are no guidelines available in the literature for assessing the suitability of material and its compaction state for constructing the surface layer based on erosion resistance. Even after following the strength and hydraulic conductivity recommendations of US Environmental Protection Agency, there are cases of cover failures which are mostly attributed to rainfall runoff-induced erosion of surface layers. Therefore, the present study demonstrates the usefulness of the soil erosion index determined by well-established pinhole test for qualifying a soil as surface layer. The effect of compaction and energy of flow on erosion was studied in detail.
The static and dynamic behaviour of sands grouted with amino-based resin
Authors: Halabian Amir M. , Shakibzadeh Arian , Rowshan Zamir Mohammad Ali
Published: September 2017

The work investigated the effect of using an amino-based resin as a new chemical grout on the static and dynamic behaviour of dry and saturated sands. Several samples of a uniformly graded silty sand having different relative densities were injected with this resinous grouting. Permeability tests conducted on the grouted samples showed that the amino-based resin reduced the permeability. To determine the effect of grouting on the static behaviour of grouted samples, a set of uniaxial compression, unconsolidated undrained and consolidated undrained triaxial tests were performed on specimens with different relative dry densities. Undrained cyclic triaxial tests were also performed to study the dynamic properties of grouted sands. The cyclic compression behaviour of the samples was investigated under stress-controlled harmonic loading with different dynamic load amplitudes and frequencies of 0·5, 1·0 and 5 Hz. The initial shear modulus and the damping ratio of the grouted samples were evaluated in terms of the number of loading cycles. The results showed that compression static and dynamic strengths increased with increasing relative density and curing time. It was also found that the relative dry density had no effect on the pulse velocity of the grouted sands.