bluuebunny/crossref_metadata_2025 huggingface dataset:
The use of B-splines to represent the topography of river networks
Authors: Boergens Eva , Schmidt Michael , Seitz Florian
Published: September 2021

This work presents a new extension to B-Splines that enables them to model functions on directed tree graphs such as non-braided river networks. The main challenge of the application of B-splines to graphs is their definition in the neighbourhood of nodes with more than two incident edges. Achieving that the B-splines are continuous at these points is non-trivial. For both, simplification reasons and in view of our application, we limit the graphs to directed tree graphs. To fulfil the requirement of continuity, the knots defining the B-Splines need to be located symmetrically along the edges with the same direction. With such defined B-Splines, we approximate the topography of the Mekong River system from scattered height data along the river. To this end, we first test and validate successfully the method with synthetic water level data, with and without added annual signal. The quality of the resulting heights is assessed besides others by means of root mean square errors (RMSE) and mean absolute differences (MAD). The RMSE values are 0.26 m and 1.05 m without and with added annual variation respectively and the MAD values are even lower with 0.11 m and 0.60 m. For the second test, we use real water level observations measured by satellite altimetry. Again, we successfully estimate the river topography, but also discuss the short comings and problems with unevenly distributed data. The unevenly distributed data leads to some very large outliers close to the upstream ends of the rivers tributaries and in regions with rapidly changing topography such as the Mekong Falls. Without the outlier removal the standard deviation of the resulting heights can be as large as 50 m with a mean value of 15.73 m. After the outlier removal the mean standard deviation drops to 8.34 m.
Uniform approximations of the first symmetric elliptic integral in terms of elementary functions
Authors: Bujanda Blanca , López José L. , Pagola Pedro J. , Palacios Pablo
Published: September 2021

We consider the standard symmetric elliptic integral $$R_F(x,y,z)$$

R
F

(
x
,
y
,
z
)

for complex x, y, z. We derive convergent expansions of $$R_F(x,y,z)$$

R
F

(
x
,
y
,
z
)

in terms of elementary functions that hold uniformly for one of the three variables x, y or z in closed subsets (possibly unbounded) of $$\mathbb {C}{\setminus }(-\infty ,0]$$

C
\
(
-
∞
,
0
]

. The expansions are accompanied by error bounds. The accuracy of the expansions and their uniform features are illustrated by means of some numerical examples.
Rail RCF damage quantification and comparison for different damage models
Authors: Krishna Visakh V. , Hossein-Nia Saeed , Casanueva Carlos , Stichel Sebastian , Trummer Gerald , Six Klaus
Published: September 2021

There are several fatigue-based approaches that estimate the evolution of rolling contact fatigue (RCF) on rails over time and built to be used in tandem with multi-body simulations of vehicle dynamics. However, most of the models are not directly comparable with each other since they are based on different physical models even though they shall predict the same RCF damage at the end. This article studies different approaches to quantifying RCF and puts forward a measure for the degree of agreement between them. The methodological framework studies various steps in the RCF quantification procedure within the context of one another, identifies the ‘primary quantification step’ in each approach and compares results of the fatigue analyses. In addition to this, two quantities—‘similarity’ and ‘correlation’—have been put forward to give an indication of mutual agreement between models. Four widely used surface-based and sub-surface-based fatigue quantification approaches with varying complexities have been studied. Different operational cases corresponding to a metro vehicle operation in Austria have been considered for this study. Results showed that the best possible quantity to compare is the normalized damage increment per loading cycle coming from different approaches. Amongst the methods studied, approaches that included the load distribution step on the contact patch showed higher similarity and correlation in their results. While the different approaches might qualitatively agree on whether contact cases are ‘damaging’ due to RCF, they might not quantitatively correlate with the trends observed for damage increment values.
Relationship between microstructure and deformation of porous Ni-based cermets under redox cycling
Authors: Sato Kazuhisa , Watanabe Satoshi , Huang Yihui , Miyasaka Taihei , Matsui Toshiaki , Yashiro Keiji , Kawada Tatsuya , Amezawa Koji , Kumada Keigo , Eguchi Koichi
Published: September 2021

This paper discusses the relationship between the elongation and compression behavior and microstructural changes under redox cycles of porous Ni(O)–YSZ cermets for solid oxide fuel cells (SOFC). Mechanical damage in SOFC and SOEC is one of the most important degradation factors governing the electrical performance of cells. Therefore, it is necessary to know the mechanical properties of each component material, such as elastic and deformation properties, in the operating environment. Particularly, of the Ni(O)–YSZ cermets which currently makes up 90% of the volume of the cell, with present mainstream anode supported SOFC and SOEC. Therefore, understanding the properties of the Ni(O)–YSZ cermets plays an important role in ensuring the performance of the entire SOFC and SOEC. In this study, the microstructural changes of Ni(O)–YSZ cermet by reduction, re-oxidation and re-reduction were observed in detail using microstructural observations and systematically compared with the dimensional change behavior. For the dimensional change behavior, a simple model considering the initial porosity and Ni content is proposed, which successfully predicts the dimensional change due to re-oxidation. Furthermore, Ni(O)–YSZ cermets with high Ni content show large initial dimensional changes, but the dimensional reversibility improves with increase of the number of redox cycles.
Climate change adaptation in conflict-affected countries: A systematic assessment of evidence
Authors: Sitati A. , Joe E. , Pentz B. , Grayson C. , Jaime C. , Gilmore E. , Galappaththi E. , Hudson A. , Alverio G. Nagle , Mach K. J. , van Aalst M. , Simpson N. , Schwerdtle P. Nayna , Templeman S. , Zommers Z. , Ajibade I. , Chalkasra L. S. Safaee , Umunay P. , Togola I. , Khouzam A. , Scarpa G. ,   Global Adaptation Mapping Initiative Team, de Perez E. Coughlan
Published: September 2021

People affected by conflict are particularly vulnerable to climate shocks and climate change, yet little is known about climate change adaptation in fragile contexts. While climate events are one of the many contributing drivers of conflict, feedback from conflict increases vulnerability, thereby creating conditions for a vicious cycle of conflict. In this study, we carry out a systematic review of peer-reviewed literature, taking from the Global Adaptation Mapping Initiative (GAMI) dataset to documenting climate change adaptation occurring in 15 conflict-affected countries and compare the findings with records of climate adaptation finance flows and climate-related disasters in each country. Academic literature is sparse for most conflict-affected countries, and available studies tend to have a narrow focus, particularly on agriculture-related adaptation in rural contexts and adaptation by low-income actors. In contrast, multilateral and bilateral funding for climate change adaptation addresses a greater diversity of adaptation needs, including water systems, humanitarian programming, and urban areas. Even among the conflict-affected countries selected, we find disparity, with several countries being the focus of substantial research and funding, and others seeing little to none. Results indicate that people in conflict-affected contexts are adapting to climate change, but there is a pressing need for diverse scholarship across various sectors that documents a broader range of adaptation types and their results.
Intervention programs designed to promote healthy romantic relationships in youth: A systematic review
Authors: Hielscher Emily , Moores Catherine , Blenkin Melanie , Jadambaa Amarzaya , Scott James G.
Published: September 2021

Introduction and Objectives

To conduct a systematic literature review of intervention programs designed to promote healthy romantic relationships in youth (aged 12–25 years). The focus was on universal interventions that have the potential to be effective and widely implemented.

Methods

We systematically searched PubMed, PsycINFO, Social Science Database, and Embase. Articles were included if they were a randomized controlled trial (RCT) or quasi‐experimental study and reported on a universal intervention aimed at promoting healthy romantic relationship knowledge, attitudes, and behaviors among youth.

Results

The search strategy identified 27 studies (26,212 participants). Interventions were found to be effective for improving healthy romantic relationship knowledge in the target population. However, the findings were mixed for intervention effectiveness in changing relationship attitudes/beliefs, and there was limited evidence to support change across behavioral outcomes.

Conclusions

This review highlights the need for future research, including high quality RCTs with longer follow‐up periods in a broad range of cultural and ethnic settings, to improve the generalisability of findings. Interventions for adolescents that improve knowledge and behavioral change relating to healthy romantic relationships have the potential to reduce mental and physical health problems during this phase of development.
Branded Content Experience in Social Media: Conceptualization, Scale Development, and Validation
Authors: Waqas Muhammad , Salleh Noor Akma Mohd , Hamzah Zalfa Laili
Published: September 2021

Understanding customer experience with branded content on social media has become critical as brands allocate a considerable budget to the creation and dissemination of branded content. Customers interact with social media branded content such as brand-related images, videos, and text posts, and respond by engaging with content on social media. However, despite growing scholarly interest in social media experiences, there is a lack of research on the conceptualization and operationalization of the “branded content experience” (BCE) on social media. This research aims to enhance the understanding of customer experience with branded content by exploring several experience dimensions and construct a BCE scale. We derived seven BCE dimensions, including self-identity, social bonding, utilitarian, aesthetic, humor, awe-inspiring, and discerning with the help of two qualitative studies. We employed exploratory and confirmatory factor analysis to propose a reliable and valid 35-item BCE scale. The findings suggested that BCE predicts consumer engagement with branded content (CEBC) on social media.
Impact of the WHO Surgical Safety Checklist Relative to Its Design and Intended Use: A Systematic Review and Meta-Meta-Analysis
Authors: Sotto Kenji T. , Burian Barbara K. , Brindle Mary E.
Published: September 2021

Background

The aim of this study was to identify what parts of the World Health Organization Surgical Safety Checklist (WHO SSC) are working, what can be done to make it more effective, and to determine if it achieved its intended effect relative to its design and intended use.

Study Design

We conducted a qualitative thematic analysis and meta-meta-analyses of findings in WHO SSC systematic reviews following Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines.

Results

Twenty systematic reviews were included for qualitative thematic analysis. Narrative information was coded in 4 primary areas with a focus on impact of the WHO SSC. Four themes—Clinical Outcomes, Process Measures, Team Dynamics and Communication, and Safety Culture—pertained directly to the aims or purposes behind the development of the SSC. The other 2 themes—Efficiency and Workload involved in using the checklist and Checklist Impact on Institutional Practices—are associated with SSC use, but were not focal areas considered during its development. Included in the 20 systematic reviews were 24 unique observational cohort studies that reported pre-post data on a total of 18 clinical outcomes. Mortality, morbidity, surgical site infection, pneumonia, unplanned return to the operating room, urinary tract infection, blood loss requiring transfusion, unplanned intubation, and sepsis favored the use of the WHO SSC. Deep vein thrombosis was the only postoperative outcome assessed that did not favor use of the WHO SSC.

Conclusions

The WHO SSC positively impacts the things it was explicitly designed to address and does not positively impact things it was not explicitly designed for.
Ordo-Responsibility in the Sharing Economy: A Social Contracts Perspective
Authors: Hielscher Stefan , Everding Sebastian , Pies Ingo
Published: September 2021

Can private companies legitimately regulate sharing markets, and if yes, how? Whereas scholars have either criticized sharing platforms for expanding into private and public arenas or welcomed them to counterbalance encroaching government regulations, studies document their unbridled popularity. On the basis of a special version of social contracts theory pioneered by James Buchanan, we develop a heuristics that helps guide reasoning about the legitimacy of the sharing economy’s regulatory function. First, we discuss the conditions under which free and responsible individuals deliberately subject themselves to rules without their prior direct participation, that is, exit, voice, and constitutional limits. Second, we suggest sharing platforms can use novel ordo-responsibilities to establish a sharing constitution that takes these conditions into account. Third, we argue that sharing platforms can legitimately do so within an enabling institutional environment in society, the provision of which relies on the joint efforts of sharing platforms, political actors, and civil society.
ACKERMANN’S FUNCTION IN ITERATIVE FORM: A PROOF ASSISTANT EXPERIMENT
Authors: PAULSON LAWRENCE C.
Published: September 2021

Ackermann’s function can be expressed using an iterative algorithm, which essentially takes the form of a term rewriting system. Although the termination of this algorithm is far from obvious, its equivalence to the traditional recursive formulation—and therefore its totality—has a simple proof in Isabelle/HOL. This is a small example of formalising mathematics using a proof assistant, with a focus on the treatment of difficult recursions.
Effects of meteorology and soil moisture on the spatio-temporal evolution of the depth hoar layer in the polar desert snowpack
Authors: Davesne Gautier , Domine Florent , Fortier Daniel
Published: September 2021

In polar deserts, depth hoar (hereinafter: DH) growth is not systematic unlike on tundra and this is critical for snowpack properties. Here, we address the spatio-temporal variability of the DH layer in the polar desert at two sites in the Canadian High Arctic: Ward Hunt Island (83° N) and Resolute Bay (75° N). Our data show that, over humid areas, DH represented a larger fraction of the snowpack and was characterized by lower density and coarser crystals than over dry gravelly areas. Increased soil moisture extends the zero-curtain period during freeze-up, leading to stronger temperature gradients in the snowpack and greater kinetic metamorphism. Our results also demonstrate that the large inter-annual variability in DH is primarily driven by wind conditions in the fall since this key variable controls the initial snow density and snow onset date. These strong controls exerted by soil moisture and meteorological conditions on DH growth in polar deserts highlight the possibility of major changes in polar snowpacks physical properties in response to the rapid climate and environmental changes currently affecting these regions.
Home-State Interest, Nationalism, and the Legitimacy of the International Criminal Court
Authors: Carrington Nathan T. , Sigsworth Claire
Published: September 2021

Although legitimacy is crucial for courts’ efficacy, the sources identified as legitimizing domestic institutions are weaker or absent altogether for international institutions. We use an original, preregistered, nationally representative survey experiment to show that perceived home-state interest strongly affects the legitimacy afforded by UK citizens to the International Criminal Court. Importantly, this relationship is moderated by nationalism. Our findings have implications for state actors in a position to act vis-á-vis international courts, elites seeking to alter opinions toward courts, and courts themselves weighing possible institutional costs of acting against noncompliant states.
Nonterritorial Autonomy in Northern Eurasia: Rooted or Alien?
Authors: Osipov Alexander
Published: September 2021

The article examines the ideas and arrangements referred to as nonterritorial autonomy (NTA) in the Russian Empire, the Soviet Union, and the post-Soviet states. Many scholars regard NTA as a theoretical breakthrough and as a way to drastically rearrange diversity policies. The author seeks to clarify whether NTA had been a groundbreaking innovation and an area of political contestations. Two short periods of NTA-related initiatives after 1917 and in the late 1980s–1990s may look like attempts (albeit ineffective) to replace the earlier forms of diversity governance. The author shows that the ideas of group societal separateness, differential treatment of individuals, group agency, and cohesiveness, as well as a group’s running of its internal affairs, were present in varying degrees in imperial, Soviet, and post-Soviet governments’ thoughts and practices. Academia and civil society were also appropriating and developing these views, and group self-rule on a nonterritorial basis was their logical extension. However, the practical implementation was, in most cases, on a top-down basis, and group agency and self-rule were affirmed mostly rhetorically. The continuity of discourses and practices demonstrates that NTA was an integral part of “normal” and broad ethnopolitical developments across the major historic divides in Northern Eurasia.
Topics, Concepts, and Measurement: A Crowdsourced Procedure for Validating Topics as Measures
Authors: Ying Luwei , Montgomery Jacob M. , Stewart Brandon M.
Published: September 2021

Topic models, as developed in computer science, are effective tools for exploring and summarizing large document collections. When applied in social science research, however, they are commonly used for measurement, a task that requires careful validation to ensure that the model outputs actually capture the desired concept of interest. In this paper, we review current practices for topic validation in the field and show that extensive model validation is increasingly rare, or at least not systematically reported in papers and appendices. To supplement current practices, we refine an existing crowd-sourcing method by Chang and coauthors for validating topic quality and go on to create new procedures for validating conceptual labels provided by the researcher. We illustrate our method with an analysis of Facebook posts by U.S. Senators and provide software and guidance for researchers wishing to validate their own topic models. While tailored, case-specific validation exercises will always be best, we aim to improve standard practices by providing a general-purpose tool to validate topics as measures.
How Do Electoral Incentives Affect Legislator Behavior? Evidence from U.S. State Legislatures
Authors: FOUIRNAIES ALEXANDER , HALL ANDREW B.
Published: September 2021

A classic question about democratic elections is how much they are able to influence politician behavior by forcing them to anticipate future reelection attempts, especially in contexts where voters are not paying close attention and are not well informed. We compile a new dataset containing roughly 780,000 bills, combined with more than 16 million roll-call voting records for roughly 6,000 legislators serving in U.S. state legislatures with term limits. Using an individual-level difference-in-differences design, we find that legislators who can no longer seek reelection sponsor fewer bills, are less productive on committees, and are absent for more floor votes, on average. Building a new dataset of roll-call votes and interest-group ratings, we find little evidence that legislators who cannot run for reelection systematically shift their ideological platforms. In sum, elections appear to influence how legislators allocate their effort in important ways even in low-salience environments but may have less influence on ideological positioning.
Activist-Appointed Directors
Authors: Kang Jun-Koo , Kim Hyemin , Kim Jungmin , Low Angie
Published: September 2021

We examine the value impact of independent directors nominated by activists (Activist IDs). Firms appointing Activist IDs experience larger value increases than firms appointing other directors, particularly when Activist IDs have private firm experience and when their nominators remain as shareholders. This value increase persists over a long period and is greater than that of activism events without director appointments. The increase is also higher among firms with greater monitoring needs and entrenched boards. Moreover, the appointments of Activist IDs are greeted more positively by the market, and Activist IDs obtain more favorable shareholder votes and additional future directorships.
Development of a revolute-type kinematic model for human upper limb using a matrix approach
Authors: Gillawat Anil Kumar
Published: September 2021

A mathematical model is proposed for a revolute joint mechanism with an n-degree of freedom (DOF). The matrix approach is used for finding the relation between two consecutive links to determine desired link parameters such as position, velocity and acceleration using the forward kinematic approach. The matrix approach was confirmed for a proposed 10 DOF revolute type (R-type) human upper limb model with servo motors at each joint. Two DOFs are considered each at shoulder, elbow and wrist joint, followed by four DOF for the fingers. Two DOFs were considered for metacarpophalangeal (mcp) and one DOF each for proximal interphalangeal (pip) and distal interphalangeal (dip) joints. MATLAB script function was used to evaluate the mathematical model for determining kinematic parameters for all the proposed human upper limb model joints. The simplified method for kinematic analysis proposed in this paper will further simplify the dynamic modeling of any mechanism for determining joint torques and hence, easy to design control system for joint movements.
Fourier duality in the Brascamp–Lieb inequality
Authors: BENNETT JONATHAN , JEONG EUNHEE
Published: September 2021

It was observed recently in work of Bez, Buschenhenke, Cowling, Flock and the first author, that the euclidean Brascamp–Lieb inequality satisfies a natural and useful Fourier duality property. The purpose of this paper is to establish an appropriate discrete analogue of this. Our main result identifies the Brascamp–Lieb constants on (finitely-generated) discrete abelian groups with Brascamp–Lieb constants on their (Pontryagin) duals. As will become apparent, the natural setting for this duality principle is that of locally compact abelian groups, and this raises basic questions about Brascamp–Lieb constants formulated in this generality.
Paediatric heart transplantation recipients ≥7 years of age receiving donors with pre-existing coronary atherosclerosis showed progressive coronary artery disease
Authors: Kim Mi Jin , Yu Jeong Jin , Cha Seulgi , Baek Jae Suk , Choi Eun Seok , Kwon Bo Sang , Park Chun Soo , Yun Tae-Jin , Kim Young-Hwue
Published: September 2021

Background:

This study aimed to determine the effect of donor-transmitted atherosclerosis on the late aggravation of cardiac allograft vasculopathy in paediatric heart recipients aged ≥7 years.

Methods:

In total, 48 patients were included and 23 had donor-transmitted atherosclerosis (baseline maximal intimal thickness of >0.5 mm on intravascular ultrasonography). Logistic regression analyses were performed to identify risk factors for donor-transmitted atherosclerosis. Rates of survival free from the late aggravation of cardiac allograft vasculopathy (new or worsening cardiac allograft vasculopathy on following angiograms, starting 1 year after transplantation) in each patient group were estimated using the Kaplan–Meier method and compared using the log-rank test. The effect of the results of intravascular ultrasonography at 1 year after transplantation on the late aggravation of cardiac allograft vasculopathy, correcting for possible covariates including donor-transmitted atherosclerosis, was examined using the Cox proportional hazards model.

Results:

The mean follow-up duration after transplantation was 5.97 ± 3.58 years. The log-rank test showed that patients with donor-transmitted atherosclerosis had worse survival outcomes than those without (p = 0.008). Per the multivariate model considering the difference of maximal intimal thickness between baseline and 1 year following transplantation (hazard ratio, 22.985; 95% confidence interval, 1.948–271.250; p = 0.013), donor-transmitted atherosclerosis was a significant covariate (hazard ratio, 4.013; 95% confidence interval, 1.047–15.376; p = 0.043).

Conclusion:

Paediatric heart transplantation recipients with donor-transmitted atherosclerosis aged ≥7 years had worse late cardiac allograft vasculopathy aggravation-free survival outcomes.
Do children with congenital heart defects meet the vaccination recommendations? Immunisation in children with congenital heart defects
Authors: Remmele Julia , Westphal Dominik S. , Unterleitner Carolin , Becker Romy , Oberhoffer-Fritz Renate , Hager Alfred , Ewert Peter , Wacker-Gussmann Annette
Published: September 2021

Introduction:

Congenital heart defects (CHDs) are the most common congenital malformations. Patients with CHD have a higher morbidity and mortality rate and are at greater risk for infectious diseases. The risk might even be higher if complex CHD occurs and if CHD is associated with additional co-morbidities. Therefore, immunisations in these children are essential.

Materials and Methods:

Individuals were recruited at the outpatient centre of the Department of Congenital Heart Defects and Pediatric Cardiology at the German Heart Center Munich in the time between February 2016 and February 2017. Included were children between 23 months and 17 years and a diagnosis of CHD. The vaccination certificate aimed to assess the immunization status.

Results:

In total, 657 children with CHD were included and analysed. Regarding primary immunisation, only 34 % (n = 221) of the children reached the complete vaccination status within the allowed catch-up time. Among these primary immunisation rates, vaccinations against Hepatitis B, Meningococci, Varicella and Pneumococci were found to have the lowest coverage with all being below 80%. The vaccination rate was partly influenced by the previously performed number of surgeries but not by the diagnosis of specific genetic diseases. At the age of school entry, the immunisation rate in children with CHD was also lower than in the comparable healthy population.

Conclusion:

The vaccination coverage rate in children with CHD is lower than in comparable healthy children, although this is a vulnerable patient group. Further education of parents and treating physicians of children with CHD regarding vaccination is still needed.
Influence of parental physical activity on offspring’s nutritional status: an intergenerational study in the 1993 Pelotas birth cohort
Authors: Blumenberg Cauane , Martins Rafaela Costa , da Silva Shana Ginar , da Silva Bruna Gonçalves Cordeiro , Wehrmeister Fernando C , Gonçalves Helen , Hallal Pedro C , Crochemore-Silva Inácio , Menezes Ana MB
Published: September 2021

Objective:

To investigate the influence of parental physical activity on offspring’s nutritional status in the 1993 Pelotas (Brazil) birth cohort.

Design:

Birth cohort study.

Setting:

The main outcomes were overweight and obesity status of children. The main exposure was parental physical activity over time, measured during the 11, 15 and 18 years of age follow-ups. The exposure was operationalised as cumulative, and the most recent measure before the birth of child. We adjusted Poisson regression models with robust variance to evaluate crude and adjusted associations between parental physical activity and offspring’s nutritional status. All analyses were stratified according to the sex of the parent.

Participants:

A total of 874 members from the 1993 Pelotas (Brazil) birth cohort followed-up at 22 years of age with their first-born child were analysed.

Results:

Children were, on average, 3·1 years old. Crude analyses showed that the mother’s cumulative physical activity measure had an indirect association with the prevalence of children’s obesity. The most recent maternal physical activity measure before the birth of the child was associated with 41 % lower prevalence of obesity in children, even after adjustment for confounders.

Conclusions:

The most recent maternal physical activity measure was indirectly associated with the prevalence of obesity in children. No associations were found for fathers, reinforcing the hypothesis of a biological effect of maternal physical activity on offspring’s nutritional status.
Retrospective analysis of portal dosimetry pre-treatment quality assurance of intracranial SRS/SRT VMAT treatment plans
Authors: Osei Ernest , Graves Sarah , Darko Johnson
Published: September 2021

Background:

The complexity associated with the treatment planning and delivery of stereotactic radiosurgery (SRS) or stereotactic radiotherapy (SRT) volumetric modulated arc therapy (VMAT) plans which employs continuous dynamic modulation of dose rate, field aperture and gantry speed necessitates diligent pre-treatment patient-specific quality assurance (QA). Numerous techniques for pre-treatment VMAT treatment plans QA are currently available with the aid of several different devices including the electronic portal imager (EPID). Although several studies have provided recommendations for gamma criteria for VMAT pre-treatment QA, there are no specifics for SRS/SRT VMAT QA. Thus, we conducted a study to evaluate intracranial SRS/SRT VMAT QA to determine clinical action levels for gamma criteria based on the institutional estimated means and standard deviations.

Materials and methods:

We conducted a retrospective analysis of 118 EPID patient-specific pre-treatment QA dosimetric measurements of 47 brain SRS/SRT VMAT treatment plans using the integrated Varian solution (RapidArcTM planning, EPID and Portal dosimetry system) for planning, delivery and EPID QA analysis. We evaluated the maximum gamma (γmax), average gamma (γave) and percentage gamma passing rate (%GP) for different distance-to-agreement/dose difference (DTA/DD) criteria and low-dose thresholds.

Results:

The gamma index analysis shows that for patient-specific SRS/SRT VMAT QA with the portal dosimetry, the mean %GP is ≥98% for 2–3 mm/1–3% and Field+0%, +5% and +10% low-dose thresholds. When applying stricter spatial criteria of 1 mm, the mean %GP is >90% for DD of 2–3% and ≥88% for DD of 1%. The mean γmax ranges: 1·32 ± 1·33–2·63 ± 2·35 for 3 mm/1–3%, 1·57 ± 1·36–2·87 ± 2·29 for 2 mm/1–3% and 2·36 ± 1·83–3·58 ± 2·23 for 1 mm/1–3%. Similarly the mean γave ranges: 0·16 ± 0·06–0·19 ± 0·07 for 3 mm/1–3%, 0·21 ± 0·08–0·27 ± 0·10 for 2 mm/1–3% and 0·34 ± 0·14–0·49 ± 0·17 for 1 mm/1–3%. The mean γmax and mean γave increase with increased DTA and increased DD for all low-dose thresholds.

Conclusions:

The establishment of gamma criteria local action levels for SRS/SRT VMAT pre-treatment QA based on institutional resources is imperative as a useful tool for standardising the evaluation of EPID-based patient-specific SRS/SRT VMAT QA. Our data suggest that for intracranial SRS/SRT VMAT QA measured with the EPID, a stricter gamma criterion of 1 mm/2% or 1 mm/3% with ≥90% %GP could be used while still maintaining an in-control QA process with no extra burden on resources and time constraints.
Determining Action Reversibility in STRIPS Using Answer Set and Epistemic Logic Programming
Authors: FABER WOLFGANG , MORAK MICHAEL , CHRPA LUKÁŠ
Published: September 2021

In the context of planning and reasoning about actions and change, we call an action reversible when its effects can be reverted by applying other actions, returning to the original state. Renewed interest in this area has led to several results in the context of the PDDL language, widely used for describing planning tasks. In this paper, we propose several solutions to the computational problem of deciding the reversibility of an action. In particular, we leverage an existing translation from PDDL to Answer Set Programming (ASP), and then use several different encodings to tackle the problem of action reversibility for the STRIPS fragment of PDDL. For these, we use ASP, as well as Epistemic Logic Programming (ELP), an extension of ASP with epistemic operators, and compare and contrast their strengths and weaknesses.
Climate Change Can Accelerate Depletion of Montane Grassland C Stocks
Authors: Wang Na , Xia Longlong , Goodale Christine L. , Butterbach‐Bahl Klaus , Kiese Ralf
Published: September 2021

Climate warming and management will likely affect carbon (C) fluxes of montane grassland ecosystems. In this study, we assessed the effect of simultaneous warming (+2°C) and decreased precipitation (−25%) on carbon exchange of montane grasslands in S‐Germany by translocating large intact plant‐soil cores from a high altitude to a low altitude site. Cores received two common grassland management regimes: intensive (4–5 cuts and slurry application) and extensive (1–2 cuts and slurry application). Diurnal patterns of net ecosystem exchange (NEE) and total ecosystem respiration (Reco) were measured over 1.5 years in 2–3 weeks intervals during the snow free period. Additional data on environmental controls, that is, photosynthetic active radiation, grass height and soil moisture and temperature, were used to develop empirical models to estimate daily and annual fluxes of gross primary production (GPP) and Reco. Considering the 2 years period (2014 and 2015), we found that, under warmer and slightly drier conditions, both GPP and Reco significantly (p < 0.01) increased (up to 20%) but with a higher temperature sensitivity of Reco, particularly in intensive managed grassland. The higher temperature sensitivity of Reco reduced the NEE by 0.7 t C ha−1 yr−1 for both extensive and intensive management, respectively. Considering additional carbon inputs via slurry and exports via harvest (i.e., annual net ecosystem carbon budget), our results showed that managed grasslands are already a source of C under current climate conditions (1.7–1.8 t ha−1 yr−1) which significantly (p < 0.05) increased under climate warming (2.3–2.9 t ha−1 yr−1).
Microbes on decomposing litter in streams: entering on the leaf or colonizing in the water?
Authors: Hayer Michaela , Wymore Adam S , Hungate Bruce A , Schwartz Egbert , Koch Benjamin J , Marks Jane C
Published: September 2021

When leaves fall in rivers, microbial decomposition commences within hours. Microbial assemblages comprising hundreds of species of fungi and bacteria can vary with stream conditions, leaf litter species, and decomposition stage. In terrestrial ecosystems, fungi and bacteria that enter soils with dead leaves often play prominent roles in decomposition, but their role in aquatic decomposition is less known. Here, we test whether fungi and bacteria that enter streams on senesced leaves are growing during decomposition and compare their abundances and growth to bacteria and fungi that colonize leaves in the water. We employ quantitative stable isotope probing to identify growing microbes across four leaf litter species and two decomposition times. We find that most of the growing fungal species on decomposing leaves enter the water with the leaf, whereas most growing bacteria colonize from the water column. Results indicate that the majority of bacteria found on litter are growing, whereas the majority of fungi are dormant. Both bacterial and fungal assemblages differed with leaf type on the dried leaves and throughout decomposition. This research demonstrates the importance of fungal species that enter with the leaf on aquatic decomposition and the prominence of bacteria that colonize decomposing leaves in the water.
METTL14 aggravates podocyte injury and glomerulopathy progression through N6-methyladenosine-dependent downregulating of Sirt1
Authors: Lu Zhihui , Liu Hong , Song Nana , Liang Yiran , Zhu Jiaming , Chen Jing , Ning Yichun , Hu Jiachang , Fang Yi , Teng Jie , Zou Jianzhou , Dai Yan , Ding Xiaoqiang
Published: September 2021

Podocytes are known to play a determining role in the progression of proteinuric kidney disease. N6-methyladenosine (m6A), as the most abundant chemical modification in eukaryotic mRNA, has been reported to participate in various pathological processes. However, its role in podocyte injury remains unclear. In this study, we observed the elevated m6A RNA levels and the most upregulated METTL14 expression in kidneys of mice with adriamycin (ADR) and diabetic nephropathy. METTL14 was also evidently increased in renal biopsy samples from patients with focal segmental glomerulosclerosis (FSGS) and diabetic nephropathy and in cultured human podocytes with ADR or advanced glycation end product (AGE) treatment in vitro. Functionally, we generated mice with podocyte-specific METTL14 deletion, and identified METTL14 knockout in podocytes improved glomerular function and alleviated podocyte injury, characterized by activation of autophagy and inhibition of apoptosis and inflammation, in mice with ADR nephropathy. Similar to the results in vivo, knockdown of METTL14 facilitated autophagy and alleviated apoptosis and inflammation in podocytes under ADR or AGE condition in vitro. Mechanically, we identified METTL14 knockdown upregulated the level of Sirt1, a well-known protective deacetylase in proteinuric kidney diseases, in podocytes with ADR or AGE treatment. The results of MeRIP-qPCR and dual-luciferase reporter assay indicated METTL14 promoted Sirt1 mRNA m6A modification and degradation in injured podocytes. Our findings suggest METTL14-dependent RNA m6A modification contributes to podocyte injury through posttranscriptional regulation of Sirt1 mRNA, which provide a potential approach for the diagnosis and treatment of podocytopathies.
Pegasus, a small extracellular peptide enhancing short-range diffusion of Wingless
Authors: Magny Emile G. , Platero Ana Isabel , Bishop Sarah A. , Pueyo Jose I. , Aguilar-Hidalgo Daniel , Couso Juan Pablo
Published: September 2021

Small Open Reading Frames (smORFs) coding for peptides of less than 100 amino-acids are an enigmatic and pervasive gene class, found in the tens of thousands in metazoan genomes. Here we reveal a short 80 amino-acid peptide (Pegasus) which enhances Wingless/Wnt1 protein short-range diffusion and signalling. During Drosophila wing development, Wingless has sequential functions, including late induction of proneural gene expression and wing margin development. Pegasus mutants produce wing margin defects and proneural expression loss similar to those of Wingless. Pegasus is secreted, and co-localizes and co-immunoprecipitates with Wingless, suggesting their physical interaction. Finally, measurements of fixed and in-vivo Wingless gradients support that Pegasus increases Wingless diffusion in order to enhance its signalling. Our results unveil a new element in Wingless signalling and clarify the patterning role of Wingless diffusion, while corroborating the link between small open reading frame peptides, and regulation of known proteins with membrane-related functions.
Pathway of Hsp70 interactions at the ribosome
Authors: Lee Kanghyun , Ziegelhoffer Thomas , Delewski Wojciech , Berger Scott E. , Sabat Grzegorz , Craig Elizabeth A.
Published: September 2021

In eukaryotes, an Hsp70 molecular chaperone triad assists folding of nascent chains emerging from the ribosome tunnel. In fungi, the triad consists of canonical Hsp70 Ssb, atypical Hsp70 Ssz1 and J-domain protein cochaperone Zuo1. Zuo1 binds the ribosome at the tunnel exit. Zuo1 also binds Ssz1, tethering it to the ribosome, while its J-domain stimulates Ssb’s ATPase activity to drive efficient nascent chain interaction. But the function of Ssz1 and how Ssb engages at the ribosome are not well understood. Employing in vivo site-specific crosslinking, we found that Ssb(ATP) heterodimerizes with Ssz1. Ssb, in a manner consistent with the ADP conformation, also crosslinks to ribosomal proteins across the tunnel exit from Zuo1. These two modes of Hsp70 Ssb interaction at the ribosome suggest a functionally efficient interaction pathway: first, Ssb(ATP) with Ssz1, allowing optimal J-domain and nascent chain engagement; then, after ATP hydrolysis, Ssb(ADP) directly with the ribosome.
Surface state-induced barrierless carrier injection in quantum dot electroluminescent devices
Authors: Lee Hyeonjun , Jeong Byeong Guk , Bae Wan Ki , Lee Doh C. , Lim Jaehoon
Published: September 2021

The past decade has witnessed remarkable progress in the device efficiency of quantum dot light-emitting diodes based on the framework of organic-inorganic hybrid device structure. The striking improvement notwithstanding, the following conundrum remains underexplored: state-of-the-art devices with seemingly unfavorable energy landscape exhibit barrierless hole injection initiated even at sub-band gap voltages. Here, we unravel that the cause of barrierless hole injection stems from the Fermi level alignment derived by the surface states. The reorganized energy landscape provides macroscopic electrostatic potential gain to promote hole injection to quantum dots. The energy level alignment surpasses the Coulombic attraction induced by a charge employed in quantum dots which adjust the local carrier injection barrier of opposite charges by a relatively small margin. Our finding elucidates how quantum dots accommodate barrierless carrier injection and paves the way to a generalized design principle for efficient electroluminescent devices employing nanocrystal emitters.
Spatial immunophenotypes predict response to anti-PD1 treatment and capture distinct paths of T cell evasion in triple negative breast cancer
Authors: Hammerl Dora , Martens John W. M. , Timmermans Mieke , Smid Marcel , Trapman-Jansen Anita M. , Foekens Renée , Isaeva Olga I. , Voorwerk Leonie , Balcioglu Hayri E. , Wijers Rebecca , Nederlof Iris , Salgado Roberto , Horlings Hugo , Kok Marleen , Debets Reno
Published: September 2021

Only a subgroup of triple-negative breast cancer (TNBC) responds to immune checkpoint inhibitors (ICI). To better understand lack of response to ICI, we analyze 681 TNBCs for spatial immune cell contextures in relation to clinical outcomes and pathways of T cell evasion. Excluded, ignored and inflamed phenotypes can be captured by a gene classifier that predicts prognosis of various cancers as well as anti-PD1 response of metastatic TNBC patients in a phase II trial. The excluded phenotype, which is associated with resistance to anti-PD1, demonstrates deposits of collagen-10, enhanced glycolysis, and activation of TGFβ/VEGF pathways; the ignored phenotype, also associated with resistance to anti-PD1, shows either high density of CD163+ myeloid cells or activation of WNT/PPARγ pathways; whereas the inflamed phenotype, which is associated with response to anti-PD1, revealed necrosis, high density of CLEC9A+ dendritic cells, high TCR clonality independent of neo-antigens, and enhanced expression of T cell co-inhibitory receptors.
In vivo inducible reverse genetics in patients’ tumors to identify individual therapeutic targets
Authors: Carlet Michela , Völse Kerstin , Vergalli Jenny , Becker Martin , Herold Tobias , Arner Anja , Senft Daniela , Jurinovic Vindi , Liu Wen-Hsin , Gao Yuqiao , Dill Veronika , Fehse Boris , Baldus Claudia D. , Bastian Lorenz , Lenk Lennart , Schewe Denis M. , Bagnoli Johannes W. , Vick Binje , Schmid Jan Philipp , Wilhelm Alexander , Marschalek Rolf , Jost Philipp J. , Miething Cornelius , Riecken Kristoffer , Schmidt-Supprian Marc , Binder Vera , Jeremias Irmela
Published: September 2021

High-throughput sequencing describes multiple alterations in individual tumors, but their functional relevance is often unclear. Clinic-close, individualized molecular model systems are required for functional validation and to identify therapeutic targets of high significance for each patient. Here, we establish a Cre-ERT2-loxP (causes recombination, estrogen receptor mutant T2, locus of X-over P1) based inducible RNAi- (ribonucleic acid interference) mediated gene silencing system in patient-derived xenograft (PDX) models of acute leukemias in vivo. Mimicking anti-cancer therapy in patients, gene inhibition is initiated in mice harboring orthotopic tumors. In fluorochrome guided, competitive in vivo trials, silencing of the apoptosis regulator MCL1 (myeloid cell leukemia sequence 1) correlates to pharmacological MCL1 inhibition in patients´ tumors, demonstrating the ability of the method to detect therapeutic vulnerabilities. The technique identifies a major tumor-maintaining potency of the MLL-AF4 (mixed lineage leukemia, ALL1-fused gene from chromosome 4) fusion, restricted to samples carrying the translocation. DUX4 (double homeobox 4) plays an essential role in patients’ leukemias carrying the recently described DUX4-IGH (immunoglobulin heavy chain) translocation, while the downstream mediator DDIT4L (DNA-damage-inducible transcript 4 like) is identified as therapeutic vulnerability. By individualizing functional genomics in established tumors in vivo, our technique decisively complements the value chain of precision oncology. Being broadly applicable to tumors of all kinds, it will considerably reinforce personalizing anti-cancer treatment in the future.
Surface lattice engineering for fine-tuned spatial configuration of nanocrystals
Authors: Jiang Bo , Yuan Yifei , Wang Wei , He Kun , Zou Chao , Chen Wei , Yang Yun , Wang Shun , Yurkiv Vitaliy , Lu Jun
Published: September 2021

Hybrid nanocrystals combining different properties together are important multifunctional materials that underpin further development in catalysis, energy storage, et al., and they are often constructed using heterogeneous seeded growth. Their spatial configuration (shape, composition, and dimension) is primarily determined by the heterogeneous deposition process which depends on the lattice mismatch between deposited material and seed. Precise control of nanocrystals spatial configuration is crucial to applications, but suffers from the limited tunability of lattice mismatch. Here, we demonstrate that surface lattice engineering can be used to break this bottleneck. Surface lattices of various Au nanocrystal seeds are fine-tuned using this strategy regardless of their shape, size, and crystalline structure, creating adjustable lattice mismatch for subsequent growth of other metals; hence, diverse hybrid nanocrystals with fine-tuned spatial configuration can be synthesized. This study may pave a general approach for rationally designing and constructing target nanocrystals including metal, semiconductor, and oxide.