bluuebunny/crossref_metadata_2025 huggingface dataset:
SMARTS
Authors: Ramamohanarao Kotagiri , Xie Hairuo , Kulik Lars , Karunasekera Shanika , Tanin Egemen , Zhang Rui , Khunayn Eman Bin
Published: December 2016

Microscopic traffic simulators are important tools for studying transportation systems as they describe the evolution of traffic to the highest level of detail. A major challenge to microscopic simulators is the slow simulation speed due to the complexity of traffic models. We have developed the Scalable Microscopic Adaptive Road Traffic Simulator (SMARTS), a distributed microscopic traffic simulator that can utilize multiple independent processes in parallel. SMARTS can perform fast large-scale simulations. For example, when simulating 1 million vehicles in an area the size of Melbourne, the system runs 1.14 times faster than real time with 30 computing nodes and 0.2s simulation timestep. SMARTS supports various driver models and traffic rules, such as the car-following model and lane-changing model, which can be driver dependent. It can simulate multiple vehicle types, including bus and tram. The simulator is equipped with a wide range of features that help to customize, calibrate, and monitor simulations. Simulations are accurate and confirm with real traffic behaviours. For example, it achieves 79.1% accuracy in predicting traffic on a 10km freeway 90 minutes into the future. The simulator can be used for predictive traffic advisories as well as traffic management decisions as simulations complete well ahead of real time. SMARTS can be easily deployed to different operating systems as it is developed with the standard Java libraries.
Advanced Security Testbed Framework for Wearable IoT Devices
Authors: Siboni Shachar , Shabtai Asaf , Tippenhauer Nils O. , Lee Jemin , Elovici Yuval
Published: December 2016

Analyzing the security of Wearable Internet-of-Things (WIoT) devices is considered a complex task due to their heterogeneous nature. In addition, there is currently no mechanism that performs security testing for WIoT devices in different contexts. In this article, we propose an innovative security testbed framework targeted at wearable devices, where a set of security tests are conducted, and a dynamic analysis is performed by realistically simulating environmental conditions in which WIoT devices operate. The architectural design of the proposed testbed and a proof-of-concept, demonstrating a preliminary analysis and the detection of context-based attacks executed by smartwatch devices, are presented.
Secure Data-Centric Access Control for Smart Grid Services Based on Publish/Subscribe Systems
Authors: Duan Li , Liu Dongxi , Zhang Yang , Chen Shiping , Liu Ren Ping , Cheng Bo , Chen Junliang
Published: December 2016

The communication systems in existing smart grids mainly take the request/reply interaction model, in which data access is under the direct control of data producers. This tightly controlled interaction model is not scalable to support complex interactions among smart grid services. On the contrary, the publish/subscribe system features a loose coupling communication infrastructure and allows indirect, anonymous and multicast interactions among smart grid services. The publish/subscribe system can thus support scalable and flexible collaboration among smart grid services. However, the access is not under the direct control of data producers, it might not be easy to implement an access control scheme for a publish/subscribe system. In this article, we propose a Data-Centric Access Control Framework (DCACF) to support secure access control in a publish/subscribe model. This framework helps to build scalable smart grid services, while keeping features of service interactions and data confidentiality at the same time. The data published in our DCACF is encrypted with a fully homomorphic encryption scheme, which allows in-grid homomorphic aggregation of the encrypted data. The encrypted data is accompanied by bloom-filter encoded control policies and access credentials to enable indirect access control. We have analyzed the correctness and security of our DCACF and evaluated its performance in a distributed environment.
Retinal microvascular network alterations: potential biomarkers of cerebrovascular and neural diseases
Authors: Cabrera DeBuc Delia , Somfai Gabor Mark , Koller Akos
Published: December 2016

Increasing evidence suggests that the conditions of retinal microvessels are indicators to a variety of cerebrovascular, neurodegenerative, psychiatric, and developmental diseases. Thus noninvasive visualization of the human retinal microcirculation offers an exceptional opportunity for the investigation of not only the retinal but also cerebral microvasculature. In this review, we show how the conditions of the retinal microvessels could be used to assess the conditions of brain microvessels because the microvascular network of the retina and brain share, in many aspects, standard features in development, morphology, function, and pathophysiology. Recent techniques and imaging modalities, such as optical coherence tomography (OCT), allow more precise visualization of various layers of the retina and its microcirculation, providing a “microscope” to brain microvessels. We also review the potential role of retinal microvessels in the risk identification of cerebrovascular and neurodegenerative diseases. The association between vision problems and cerebrovascular and neurodegenerative diseases, as well as the possible role of retinal microvascular imaging biomarkers in cerebrovascular and neurodegenerative screening, their potentials, and limitations, are also discussed.
Influence of sympathetic nerve activity on aortic hemodynamics and pulse wave velocity in women
Authors: Harvey Ronée E. , Barnes Jill N. , Hart Emma C. J. , Nicholson Wayne T. , Joyner Michael J. , Casey Darren P.
Published: December 2016

Central (aortic) blood pressure, arterial stiffness, and sympathetic nerve activity increase with age in women. However, it is unknown if the age-related increase in sympathetic activity influences aortic hemodynamics and carotid-femoral pulse wave velocity (cfPWV), an index of central aortic stiffness. The goal of this study was to determine if aortic hemodynamics and cfPWV are directly influenced by sympathetic nerve activity by measuring aortic hemodynamics, cfPWV, and muscle sympathetic nerve activity (MSNA) in women before and during autonomic ganglionic blockade with trimethaphan camsylate. We studied 12 young premenopausal (23 ± 4 yr) and 12 older postmenopausal (57 ± 3 yr) women. These women did not differ in body mass index or mean arterial pressure ( P > 0.05 for both). At baseline, postmenopausal women had higher aortic pulse pressure, augmented pressure, augmentation index adjusted for a heart rate of 75 beats/min, wasted left ventricular pressure energy, and cfPWV than young women ( P < 0.05). During ganglionic blockade, postmenopausal women had a greater decrease in these variables in comparison to young women ( P < 0.05). Additionally, baseline MSNA was negatively correlated with the reductions in aortic pulse pressure, augmented pressure, and wasted left ventricular pressure energy during ganglionic blockade in postmenopausal women ( P < 0.05) but not young women. Baseline MSNA was not correlated with the changes in augmentation index adjusted for a heart rate of 75 beats/min or cfPWV in either group ( P > 0.05 for all). Our results suggest that some aortic hemodynamic parameters are influenced by sympathetic activity to a greater extent in older postmenopausal women than in young premenopausal women.

NEW & NOTEWORTHY Autonomic ganglionic blockade results in significant decreases in multiple aortic pulse wave characteristics (e.g., augmented pressure) and central pulse wave velocity in older postmenopausal women but not in young premenopausal women. Certain aortic pulse wave parameters are negatively influenced by sympathetic activity to a greater extent in older postmenopausal women.
Reduced density and altered regulation of rat atrial L-type Ca2+current in heart failure
Authors: Bond Richard C. , Bryant Simon M. , Watson Judy J. , Hancox Jules C. , Orchard Clive H. , James Andrew F.
Published: December 2016

Constitutive regulation by PKA has recently been shown to contribute to L-type Ca2+current ( ICaL) at the ventricular t-tubule in heart failure. Conversely, reduction in constitutive regulation by PKA has been proposed to underlie the downregulation of atrial ICaLin heart failure. The hypothesis that downregulation of atrial ICaLin heart failure involves reduced channel phosphorylation was examined. Anesthetized adult male Wistar rats underwent surgical coronary artery ligation (CAL, N=10) or equivalent sham-operation (Sham, N=12). Left atrial myocytes were isolated ~18 wk postsurgery and whole cell currents recorded (holding potential=-80 mV). ICaLactivated by depolarizing pulses to voltages from -40 to +50 mV were normalized to cell capacitance and current density-voltage relations plotted. CAL cell capacitances were ~1.67-fold greater than Sham ( P ≤ 0.0001). Maximal ICaLconductance ( Gmax) was downregulated more than 2-fold in CAL vs. Sham myocytes ( P < 0.0001). Norepinephrine (1 μmol/l) increased Gmax>50% more effectively in CAL than in Sham so that differences in ICaLdensity were abolished. Differences between CAL and Sham Gmaxwere not abolished by calyculin A (100 nmol/l), suggesting that increased protein dephosphorylation did not account for ICaLdownregulation. Treatment with either H-89 (10 μmol/l) or AIP (5 μmol/l) had no effect on basal currents in Sham or CAL myocytes, indicating that, in contrast to ventricular myocytes, neither PKA nor CaMKII regulated basal ICaL. Expression of the L-type α1C-subunit, protein phosphatases 1 and 2A, and inhibitor-1 proteins was unchanged. In conclusion, reduction in PKA-dependent regulation did not contribute to downregulation of atrial ICaLin heart failure.

NEW & NOTEWORTHY Whole cell recording of L-type Ca2+currents in atrial myocytes from rat hearts subjected to coronary artery ligation compared with those from sham-operated controls reveals marked reduction in current density in heart failure without change in channel subunit expression and associated with altered phosphorylation independent of protein kinase A.
Modeling fast and slow gamma oscillations with interneurons of different subtype
Authors: Keeley Stephen , Fenton André A. , Rinzel John
Published: December 2016

Experimental and theoretical studies demonstrate that neuronal gamma oscillations crucially depend on interneurons, but current models do not consider the diversity of known interneuron subtypes. Moreover, in CA1 of the hippocampus, experimental evidence indicates the presence of multiple gamma oscillators, two of which may be coordinated by differing interneuron populations. In this article, we show that models of networks with competing interneuron populations with different postsynaptic effects are sufficient to generate, within CA1, distinct oscillatory regimes. We find that strong mutual inhibition between the interneuron populations permits distinct fast and slow gamma states, whereas weak mutual inhibition generates mixed gamma states. We develop idealized firing rate models to illuminate dynamic properties of these competitive gamma networks, and reinforce these concepts with basic spiking models. The models make several explicit predictions about gamma oscillators in CA1. Specifically, interneurons of different subtype phase-lock to different gamma states, and one population of interneurons is silenced and the other active during fast and slow gamma events. Finally, mutual inhibition between interneuron populations is necessary to generate distinct gamma states. Previous experimental studies indicate that fast and slow gamma oscillations reflect different information processing modes, although it is unclear whether these rhythms are intrinsic or imposed. The models outlined demonstrate that basic architectures can locally generate these oscillations, as well as capture other features of fast and slow gamma, including theta-phase preference and spontaneous transitions between gamma states. These models may extend to describe general dynamics in networks with diverse interneuron populations.

NEW & NOTEWORTHY The oscillatory coordination of neural signals is crucial to healthy brain function. We have developed an idealized neuronal model that generates distinct fast and slow gamma oscillations, a known feature of the rodent hippocampus. Our work provides a mechanism of this phenomenon, as well as a theoretical framework for future experiments concerning hippocampal gamma. It moreover offers a tractable model of competitive gamma oscillations that is generalizable across the nervous system.
Adenosine-dependent phrenic motor facilitation is inflammation resistant
Authors: Agosto-Marlin Ibis M. , Nichols Nicole L. , Mitchell Gordon S.
Published: December 2016

Phrenic motor facilitation (pMF), a form of respiratory plasticity, can be elicited by acute intermittent hypoxia (i.e., phrenic long-term facilitation, pLTF) or direct application of drugs to the cervical spinal cord. Moderate acute intermittent hypoxia (mAIH; 3 × 5-min episodes of 35–50 mmHg arterial Po2, 5-min normoxic intervals) induces pLTF by a serotonin-dependent mechanism; mAIH-induced pLTF is abolished by mild systemic inflammation induced by a low dose of lipopolysaccharide (LPS; 100 μg/kg ip). In contrast, severe acute intermittent hypoxia (sAIH; 3 × 5-min episodes of 25–30 mmHg arterial Po2, 5-min normoxic intervals) elicits pLTF by a distinct, adenosine-dependent mechanism. Since it is not known if systemic LPS blocks the mechanism giving rise to sAIH-induced pLTF, we tested the hypothesis that sAIH-induced pLTF and adenosine 2A (A2A) receptor-induced pMF are insensitive to mild systemic inflammation elicited by the same low dose of LPS. In agreement with our hypothesis, neither sAIH-induced pLTF nor cervical intrathecal A2A receptor agonist (CGS-21680; 200 μM, 10 μl × 3)-induced pMF were affected 24 h post-LPS. Pretreatment with intrathecal A2A receptor antagonist injections (MSX-3; 10 μM, 12 μl) blocked sAIH-induced pLTF 24 h post LPS, confirming that pLTF was adenosine dependent. Our results give insights concerning the differential impact of systemic inflammation and the functional significance of multiple cascades capable of giving rise to phrenic motor plasticity. The relative resistance of adenosine-dependent pMF to inflammation suggests that it provides a “backup” system in animals lacking serotonin-dependent pMF due to ongoing inflammation associated with systemic infections and/or neural injury.

NEW & NOTEWORTHY This study gives novel insights concerning how a mild systemic inflammation impacts phrenic motor plasticity (pMF), particularly adenosine-dependent pMF. We suggest that since this adenosine-dependent pathway is insensitive to systemic inflammation, it represents an alternative or “backup” mechanism of pMF when other mechanisms are suppressed.
Revealing unobserved factors underlying cortical activity with a rectified latent variable model applied to neural population recordings
Authors: Whiteway Matthew R. , Butts Daniel A.
Published: December 2016

The activity of sensory cortical neurons is not only driven by external stimuli but also shaped by other sources of input to the cortex. Unlike external stimuli, these other sources of input are challenging to experimentally control, or even observe, and as a result contribute to variability of neural responses to sensory stimuli. However, such sources of input are likely not “noise” and may play an integral role in sensory cortex function. Here we introduce the rectified latent variable model (RLVM) in order to identify these sources of input using simultaneously recorded cortical neuron populations. The RLVM is novel in that it employs nonnegative (rectified) latent variables and is much less restrictive in the mathematical constraints on solutions because of the use of an autoencoder neural network to initialize model parameters. We show that the RLVM outperforms principal component analysis, factor analysis, and independent component analysis, using simulated data across a range of conditions. We then apply this model to two-photon imaging of hundreds of simultaneously recorded neurons in mouse primary somatosensory cortex during a tactile discrimination task. Across many experiments, the RLVM identifies latent variables related to both the tactile stimulation as well as nonstimulus aspects of the behavioral task, with a majority of activity explained by the latter. These results suggest that properly identifying such latent variables is necessary for a full understanding of sensory cortical function and demonstrate novel methods for leveraging large population recordings to this end.

NEW & NOTEWORTHY The rapid development of neural recording technologies presents new opportunities for understanding patterns of activity across neural populations. Here we show how a latent variable model with appropriate nonlinear form can be used to identify sources of input to a neural population and infer their time courses. Furthermore, we demonstrate how these sources are related to behavioral contexts outside of direct experimental control.
Physiological Aging: Links Among Adipose Tissue Dysfunction, Diabetes, and Frailty
Authors: Stout Michael B. , Justice Jamie N. , Nicklas Barbara J. , Kirkland James L.
Published: December 2016

Advancing age is associated with progressive declines in physiological function that lead to overt chronic disease, frailty, and eventual mortality. Importantly, age-related physiological changes occur in cellularity, insulin-responsiveness, secretory profiles, and inflammatory status of adipose tissue, leading to adipose tissue dysfunction. Although the mechanisms underlying adipose tissue dysfunction are multifactorial, the consequences result in secretion of proinflammatory cytokines and chemokines, immune cell infiltration, an accumulation of senescent cells, and an increase in senescence-associated secretory phenotype (SASP). These processes synergistically promote chronic sterile inflammation, insulin resistance, and lipid redistribution away from subcutaneous adipose tissue. Without intervention, these effects contribute to age-related systemic metabolic dysfunction, physical limitations, and frailty. Thus adipose tissue dysfunction may be a fundamental contributor to the elevated risk of chronic disease, disability, and adverse health outcomes with advancing age.
Dating the Heart: Exploring Cardiomyocyte Renewal in Humans
Authors: Graham Evan , Bergmann Olaf
Published: December 2016

Regenerative mechanisms reported in the hearts of lower vertebrates have been recapitulated in the mammalian milieu, and recent studies have provided strong evidence for cardiomyocyte turnover in humans. These findings speak to an emerging consensus that adult mammalian cardiomyocytes do have the ability to divide, and it stands to reason that enrichment of this innate proliferative capacity should prove essential for complete cardiac regeneration.
Transmission of Metabolic Dysfunction Across Generations
Authors: Jaeger Kathleen , Saben Jessica L. , Moley Kelle H.
Published: December 2016

Recent human and animal studies investigating the roles of the genome, epigenome, and environmental cues have identified associations between offspring predisposition to life-long obesity/metabolic disease and epigenetic modifications such as DNA methylation. This review explores the mechanisms by which maternal exposures impair the health of not only the next generation but also potentially future generations of offspring.
Evaluation of Antibacterial, Antineoplastic, and Immunomodulatory Activity of Paullinia cupana Seeds Crude Extract and Ethyl‐Acetate Fraction
Authors: Carvalho Lidiane Vasconcelos do Nascimento , Cordeiro Marina Ferraz , e Lins Thiago Ubiratan Lins , Sampaio Maria Clara Pinheiro Duarte , de Mello Gabriela Souto Vieira , da Costa Valécia de Cassia Mendonça , Marques Leila Larisa Medeiros , Klein Traudi , de Mello João Carlos Palazzo , Cavalcanti Isabella Macário Ferro , Pitta Ivan da Rocha , Galdino da Rocha Pitta Maira , Rêgo Moacyr Jesus Barreto de Melo
Published: December 2016

Paullinia cupana (Guarana) is a native plant of Amazon region that has very traditional importance. Its seeds are rich in bioactive compounds, including tannins, which exhibit relevant properties. Objective. This study aimed to evaluate antibacterial, antineoplastic, and immunomodulatory activity of P. cupana seeds crude extract (CE) and ethyl‐acetate fraction (EAF). Methods. Antibacterial activity was evaluated by determination of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Antineoplastic activity was evaluated by MTT assays in hepatocellular carcinoma (HepG2), breast adenocarcinoma (MCF‐7), ductal carcinoma (T47‐D), non‐Hodgkin’s B cell lymphoma (Toledo), T cell leukemia (Jukart), and Acute Leukemia (HL‐60) cell lines. BALB/c mice splenocytes were treated to assess IFN‐γ, IL‐6, IL‐17, and IL‐10 levels by sandwich ELISA. Results. CE and EAF were not toxic to peripheral blood cells and splenocytes. CE and EAF fractions showed a bacteriostatic activity (MIC = 250 μg/mL) and presented IC50 values of 70.25 μg/mL and 61.18 μg/mL in HL‐60 leukemia cell line. All cytokines evaluated had their levels reduced after treatment, following dose‐response model. Discussion and Conclusion. Different biological activities were observed for both CE and EAF, suggesting P. cupana as a source of bioactive substances, especially tannins that may be used for several diseases treatments.
An Efficient Method for Solving Spread Option Pricing Problem: Numerical Analysis and Computing
Authors: Company R. , Egorova V. N. , Jódar L.
Published: December 2016

This paper deals with numerical analysis and computing of spread option pricing problem described by a two-spatial variables partial differential equation. Both European and American cases are treated. Taking advantage of a cross derivative removing technique, an explicit difference scheme is developed retaining the benefits of the one-dimensional finite difference method, preserving positivity, accuracy, and computational time efficiency. Numerical results illustrate the interest of the approach.
Analysis of the Single Toggle Jaw Crusher Force Transmission Characteristics
Authors: Oduori Moses Frank , Munyasi David Masinde , Mutuli Stephen Mwenje
Published: December 2016

This paper sets out to perform a static force analysis of the single toggle jaw crusher mechanism and to obtain the force transmission characteristics of the mechanism. In order to obtain force transmission metrics that are characteristic of the structure of the mechanism, such influences as friction, dead weight, and inertia are considered to be extraneous and neglected. Equations are obtained by considering the balance of forces at the moving joints and appropriately relating these to the input torque and the output torque. A mechanical advantage, the corresponding transmitted torque, and the variations thereof, during the cycle of motion of the mechanism, are obtained. The mechanical advantage that characterizes the mechanism is calculated as the mean value over the active crushing stroke of the mechanism. The force transmission characteristics can be used as criteria for the comparison of different jaw crusher mechanism designs in order to select the most suitable design for a given application. The equations obtained can also be used in estimating the forces sustained by the components of the mechanism.
A Rare Case of Mediterranean Spotted Fever and Encephalitis
Authors: Sousa Almeida Raquel , Pego Petra M. , Pinto Maria João , Matos Costa João
Published: December 2016

Mediterranean spotted fever is a tick-borne zoonotic disease caused byRickettsia conorii. It is transmitted by the dog tickRhipicephalus sanguineus. It usually presents as a benign self-limited disease characterized by a skin rash, high fever, and, sometimes, a characteristic ulcer at the tick bite site calledtache noir. The course of this disease is usually benign, although severe manifestations have been previously described, mainly in adults. Neurological manifestations are very unusual. We present a case of Mediterranean spotted fever with encephalitis to highlight the importance of clinical suspicion, mainly in endemic areas, the potential severity of this disease, and the need of early initiation of therapy in order to prevent severe complications.
Potential Benefits of Jujube (Zizyphus Lotus L.) Bioactive Compounds for Nutrition and Health
Authors: Abdoul-Azize Souleymane
Published: December 2016

Zizyphus lotus, belonging to the Rhamnaceae family, is a deciduous shrub which generally grows in arid and semiarid regions of the globe. In traditional medicine, Z. lotus is used as antidiabetes, sedative, bronchitis, and antidiarrhea by local populations. Recently, several scientific reports for health benefit and nutritional potential of bioactive compounds from this jujube have been reported. This plant is rich in polyphenols, cyclopeptide alkaloids, dammarane saponins, vitamins, minerals, amino acids, and polyunsaturated fatty acids. These identified compounds were supposed to be responsible for most of Z. lotus biologically relevant activities including antimicrobial, anti-inflammatory, hypoglycemic, antioxidant, and immunomodulatory effects. The aim of the present review was to give particular emphasis on the most recent findings on biological effects of the major groups of Zizyphus lotus components and their medical interest, notably for human nutrition, health benefit, and therapeutic impacts.
Study on Transition of Primary Energy Structure and Carbon Emission Reduction Targets in China Based on Markov Chain Model and GM (1, 1)
Authors: Ren Feng , Gu Lihong
Published: December 2016

The improvement of the primary energy structure has been considered as one of the important measures to achieve the carbon emissions reduction targets in China. This current paper constructed a Markov chain model, which was used to forecast the transition of primary energy structure. GM (1, 1) model and a linear regression model were used to predict the total energy consumption in 2020 and 2030. Then, the CO2emissions intensity was calculated, and the realization of carbon emissions reduction targets in China was analyzed. The findings indicated that (1) China’s nonfossil energy share in primary energy cannot be achieved naturally. (2) Part of the carbon emissions intensity in China’s commitments was not binding actually. (3) The realization of the carbon emissions peak and the reduction target of carbon emissions intensity in 2030 would need the policy intervention. In the last part of this paper, policy recommendations on carbon emissions reduction in China were provided.
Security-Based Mechanism for Proactive Routing Schema Using Game Theory Model
Authors: Amraoui Hicham , Habbani Ahmed , Hajami Abdelmajid , Bilal Essaid
Published: December 2016

Game theory may offer a useful mechanism to address many problems in mobile ad hoc networks (MANETs). One of the key concepts in the research field of such networks with Optimized Link State Routing Protocol (OLSR) is the security problem. Relying on applying game theory to study this problem, we consider two strategies during this suggested model: cooperate and not-cooperate. However, in such networks, it is not easy to identify different actions of players. In this paper, we have essentially been inspired from recent advances provided in game theory to propose a new model for security in MANETs. Our proposal presents a powerful tool with a large number of players where interactions are played multiple times. Moreover, each node keeps a cooperation rate (CR) record of other nodes to cope with the behaviors and mitigate aggregate effect of other malicious devices. Additionally, our suggested security mechanism does not only take into consideration security requirements, but also take into account system resources and network performances. The simulation results using Network Simulator 3 are presented to illustrate the effectiveness of the proposal.
Current Conveyor Based Window Comparator Circuits
Authors: Maheshwari Sudhanshu
Published: December 2016

This paper introduces a new window comparator circuit utilizing a new current conveyor and two diodes, operable at ±1.25 V and capable of accurately detecting the voltage windows. Another modified circuit with distinct binary levels suited for automatic control applications is also suggested. Exhaustive simulation results showing detection of windows, as small as 50 mV and as high as 1 V, are included. Comparisons are further drawn with the traditional operational amplifier based circuit and the new circuit is found to benefit from the use of current-mode active element, namely, Extra-X Current Controlled Current Conveyor. The proposed theory is well supported through simulation results.
Intravascular Residence Time Determination for the Cyanide Antidote Dimethyl Trisulfide in Rat by Using Liquid-Liquid Extraction Coupled with High Performance Liquid Chromatography
Authors: De Silva Deepthika , Lee Steven , Duke Anna , Angalakurthi Siva , Chou Ching-En , Ebrahimpour Afshin , Thompson David E. , Petrikovics Ilona
Published: December 2016

These studies represent the first report on the intravascular residence time determinations for the cyanide antidote dimethyl trisulfide (DMTS) in a rat model by using high performance liquid chromatography coupled with ultraviolet absorption spectroscopy (HPLC-UV). The newly developed sample preparation included liquid-liquid extraction by cyclohexanone. The calibration curves showed a linear response for DMTS concentrations between 0.010 and 0.30 mg/mL withR2= 0.9994. The limit of detection for DMTS via this extraction method was 0.010 mg/mL, and the limit of quantitation was 0.034 mg/mL. Thus this calibration curve provided a tool for determining DMTS in the range between 0.04 and 0.30 mg/mL. Rats were given 20 mg/kg DMTS dose (in 15% Polysorbate 80) intravenously, and blood samples were taken 15, 60, 90, 120, and 240 min after DMTS injections. The data points were plotted as DMTS concentration in RBCs versus time, and the intravascular residence time was determined graphically. The results indicated a half-life of 36 min in a rat model, suggesting that the circulation time is long enough to provide a reasonable time interval for cyanide antagonism.
Primary Cardiac Lymphoma: Lessons Learned from a Long Survivor
Authors: Singh Baljit , Ip Randy , Ibrahim Al-Rajjal Ahmed , Kafri Zyad , Al-Katib Ayad , Hadid Tarik
Published: December 2016

Primary cardiac lymphoma (PCL) is a rare neoplasm that involves the heart, pericardium, or both. Patients with PCL have median survival of approximately 7 months. We report a 63-year-old woman with PCL treated with chemoimmunotherapy but relapsed 7 years later. She received automated implantable cardioverter-defibrillator (AICD) prophylactically shortly after the diagnosis. She presented with a breast recidive 7 years after initial diagnosis and died of relapsed small cell lung cancer. As many patients with PCL die early in the disease course due to life-threatening arrhythmias, preemptive implantation of AICD may improve mortality and prevent early death. Chemoimmunotherapy is effective in inducing remission in patients with PCL. Late and unusual pattern of relapse may be more frequent in patients with PCL and should be explored further. This case presents one of the longest surviving patients with PCL reported in the literature.
Effect of Virtual Reality on Postural and Balance Control in Patients with Stroke: A Systematic Literature Review
Authors: Chen Ling , Lo Wai Leung Ambrose , Mao Yu Rong , Ding Ming Hui , Lin Qiang , Li Hai , Zhao Jiang Li , Xu Zhi Qin , Bian Rui Hao , Huang Dong Feng
Published: December 2016

Objective. To critically evaluate the studies that were conducted over the past 10 years and to assess the impact of virtual reality on static and dynamic balance control in the stroke population.Method. A systematic review of randomized controlled trials published between January 2006 and December 2015 was conducted. Databases searched were PubMed, Scopus, and Web of Science. Studies must have involved adult patients with stroke during acute, subacute, or chronic phase. All included studies must have assessed the impact of virtual reality programme on either static or dynamic balance ability and compared it with a control group. The Physiotherapy Evidence Database (PEDro) scale was used to assess the methodological quality of the included studies.Results. Nine studies were included in this systematic review. The PEDro scores ranged from 4 to 9 points. All studies, except one, showed significant improvement in static or dynamic balance outcomes group.Conclusions. This review provided moderate evidence to support the fact that virtual reality training is an effective adjunct to standard rehabilitation programme to improve balance for patients with chronic stroke. The effect of VR training in balance recovery is less clear in patients with acute or subacute stroke. Further research is required to investigate the optimum training intensity and frequency to achieve the desired outcome.
Changes in Blood Pressure Levels and Antihypertensive Medication Use before and after Renal Transplantation among Patients in Nairobi, Kenya: A Comparative Cross-Sectional Study
Authors: Kubo Mary N. , Kayima Joshua K. , Were Anthony J. , Ezzi Mohammed S. , McLigeyo Seth O. , Ogola Elijah N.
Published: December 2016

Objectives.To determine the changes in blood pressure levels and antihypertensive medication use in the postrenal transplantation period compared to pretransplantation one.Methods. A comparative cross-sectional study was carried out on renal transplant recipients at the Kenyatta National Hospital, a national referral hospital in Kenya. Sociodemographic details, blood pressure levels, and antihypertensive medication use before and after renal transplantation were noted. Changes in mean blood pressure levels and mean number of antihypertensive medications after renal transplantation were determined using pairedt-test.Results. 85 subjects were evaluated. Mean age was 42.4 (SD ± 12.2) years, with a male : female ratio of 1.9 : 1. Compared to the pretransplant period, significantly lower mean systolic and diastolic blood pressure levels after transplantation were noted (mean SBP 144.5 mmHg versus 131.8 mmHg; mean DBP 103.6 mmHg versus 83.5 mmHg in the pre- and posttransplant periods, respectively,p<0.001). Mean number of antihypertensive medications also reduced significantly after transplantation, with an average of 3.3 (±1.6) versus 2.1 (±0.9) in the pre- and posttransplant periods, respectively (p<0.001).Conclusion. There is a significant reduction in blood pressure levels and number of antihypertensive medications used after renal transplantation. The positive impact of renal transplantation on blood pressure control should be confirmed using prospective cohort studies of patients with end stage renal disease who then undergo renal transplantation.
Some Comparison of Solutions by Different Numerical Techniques on Mathematical Biology Problem
Authors: Paul Susmita , Mondal Sankar Prasad , Bhattacharya Paritosh , Chaudhuri Kripasindhu
Published: December 2016

We try to compare the solutions by some numerical techniques when we apply the methods on some mathematical biology problems. The Runge-Kutta-Fehlberg (RKF) method is a promising method to give an approximate solution of nonlinear ordinary differential equation systems, such as a model for insect population, one-species Lotka-Volterra model. The technique is described and illustrated by numerical examples. We modify the population models by taking the Holling type III functional response and intraspecific competition term and hence we solve it by this numerical technique and show that RKF method gives good results. We try to compare this method with the Laplace Adomian Decomposition Method (LADM) and with the exact solutions.
Weighted Complex Network Analysis of the Different Patterns of Metro Traffic Flows on Weekday and Weekend
Authors: Feng Jia , Li Xiamiao , Mao Baohua , Xu Qi , Bai Yun
Published: December 2016

We present a multilayer model to characterize the weekday and weekend patterns in terms of the spatiotemporal flow size distributions in subway networks, based on trip data and operation timetables obtained from the Beijing Subway System. We also investigate the disparity of incoming and outgoing flows at a given station to describe the different spatial structure performance between transfer and nontransfer stations. In addition, we describe the essential interactions between PFN and TFN by defining an indicator, real load. By comparing with the two patterns on weekday and weekend, we found that the substantial trends have roughly the same form, with noticeable lower sizes of flows on weekend ascribed to the essential characteristics of travel demand.
Xanthohumol Prevents DNA Damage by Dietary Carcinogens: Results of a Human Intervention Trial
Authors: Pichler Christoph , Ferk Franziska , Al-Serori Halh , Huber Wolfgang , Jäger Walter , Waldherr Monika , Mišík Miroslav , Kundi Michael , Nersesyan Armen , Herbacek Irene , Knasmueller Siegfried
Published: December 2016

Xanthohumol (XN) is a hop flavonoid contained in beers and soft drinks. In vitro and animal studies indicated that XN has DNA and cancer protective properties. To find out if it causes DNA protective effects in humans, an intervention trial was conducted in which the participants (n = 22) consumed a XN containing drink (12 mg XN/P/d). We monitored prevention of DNA damage induced by representatives of major groups of dietary carcinogens [i.e., nitrosodimethylamine (NDMA) benzo(a)pyrene (B(a)P) and the heterocyclic aromatic amine 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)]. Lymphocytes were collected before, during, and after the intervention and incubated with the carcinogens and with human liver homogenate (S9). We found substantial reduction of B(a)P and IQ (P < 0.001 for both substances) induced DNA damage after consumption of the beverage; also, with the nitrosamine a moderate, but significant protective effect was found. The results of a follow-up trial (n = 10) with XN pills showed that the effects are caused by the flavonoid and were confirmed in γH2AX experiments. To elucidate the underlying mechanisms we measured several parameters of glutathione related detoxification. We found clear induction of α-GST (by 42.8%, P < 0.05), but no alteration of π-GST. This observation provides a partial explanation for the DNA protective effects and indicates that the flavonoid also protects against other carcinogens that are detoxified by α-GST. Taken together, our findings support the assumption that XN has anticarcinogenic properties in humans. Cancer Prev Res; 10(2); 153–60. ©2016 AACR.
Open-Label Phase I Clinical Study to Assess the Safety and Efficacy of Cilostazol in Patients Undergoing Internal Carotid Artery Stent Placement
Authors: Hassan Ameer E. , Zacharatos Haralabos , Grigoryan Mikayel , Tekle Wondwossen G. , Khan Amir , Siddiq Farhan , Rodriguez Gustavo J. , Tummala Ramachandra , Jagadeesan Bharathi , Suri M. Fareed K. , Qureshi Adnan I.
Published: December 2016

Background: One-month dual antiplatelet treatment, with aspirin and clopidogrel, following internal carotid artery stent placement is the current standard of care to prevent in-stent thrombosis. Cilostazol, an antiplatelet drug, has been demonstrated to have a safety profile comparable to aspirin and clopidogrel. Objective: To evaluate the safety and clinical efficacy of cilostazol and aspirin therapy following internal carotid artery stent placement up to 1 month postprocedure. Methods: A phase I open-label, nonrandomized two-center prospective study was conducted. All subjects received aspirin (325 mg/day) and cilostazol (200 mg/day) 3 days before extracranial stent placement. Two antiplatelet agents were continued for 1 month postprocedure followed by aspirin daily monotherapy. The primary efficacy end point was the 30-day composite occurrence of death, cerebral infarction, transient ischemic attack, and in-stent thrombosis. The primary safety end point was bleeding. Results: Twelve subjects (mean age ± SD, 66 ± 12 years; 9 males) were enrolled and underwent internal carotid artery angioplasty and stent placement. None of the subjects who successfully followed the study protocol experienced any complications at the 1- and 3-month follow-ups. One patient had a protocol deviation due to concurrent use of enoxaparin (1 mg/kg twice daily) in addition to aspirin and cilostazol, resulting in a fatal symptomatic intracerebral hemorrhage following successful stent placement on postprocedure day 1. One patient discontinued cilostazol after the first dose secondary to dizziness. Conclusion: The use of cilostazol and aspirin for internal carotid artery stent placement appears to be safe, but protocol compliance needs to be emphasized.
Clinical Course of Acute Kidney Injury in Elderly Individuals Above 80 Years
Authors: Funk Isabell , Seibert Eric , Markau Silke , Girndt Matthias
Published: December 2016

Background/Aims: Aging is associated with renal function decline and elderly patients are more vulnerable to acute kidney injury (AKI). The causes and prognosis of AKI according to new KDIGO definition that broadened the diagnosis and included more patients without dialysis dependence have not yet been compared between younger and elderly patients. Methods: In a retrospective analysis all patients with AKI admitted to a tertiary care Nephrology department (N=424) were included. Individuals were stratified by age (≤80 years, >80 years). Primary end-point was death or dialysis dependence at hospital discharge, secondary analyses addressed the need for dialysis, creatinine at discharge, mortality, and length of stay. Results: The distribution of AKI causes was different between the age groups. Circulatory AKI was the most important cause in both groups; however, septic or toxic AKI contributed relevantly in younger patients. Nevertheless, the number of patients reaching the primary end-point was similar (younger, 20.4%; older, 18.0%; OR 1.17, 95%CI, 0.703-1.948). While mortality tended to be higher in the older population, none of the secondary analyses indicated worse outcome for the older patients. Conclusion: The prognosis of AKI in elderly patients is not necessarily worse than in middle aged individuals. Nevertheless, older patients may be particularly vulnerable to circulatory or ischemic insults of the kidneys.
Pyoderma Gangrenosum and Erythema Nodosum Revealing Takayasu’s Arteritis
Authors: Loetscher Jonas , Fistarol Susanna , Walker Ulrich A.
Published: December 2016

We report a Caucasian female who presented with simultaneous erythema nodosum and pyoderma gangrenosum due to underlying Takayasu’s arteritis. Takayasu’s arteritis is a chronic large vessel vasculitis of unknown cause. The disease has a worldwide distribution but is most commonly seen in Asian populations. There is a strong predilection for young females. The clinical presentation is variable, but mostly derives from stenosis or occlusion of affected arteries, resulting in claudication and ischemia. Skin manifestations are observed in up to 28% of patients with Takayasu’s arteritis, with erythema nodosum reported more frequently in Caucasians. Pyoderma gangrenosum is more common in Asian patients. This report demonstrates the importance to exclude Takayasu’s arteritis in patients with such skin lesions.
Scrotum Melanoma: Evolution Over One Year in This Unusual Location
Authors: Marzullo Zucchet Leopoldo José , Bonillo García Miguel Ángel , Lorenzo Soriano Laura , Broseta Rico Enrique
Published: December 2016

Genitourinary melanoma accounts for 0.1-0.2% of melanoma, the scrotum being its rarest location. We report about an 85-year-old patient who was referred to our outpatient clinic due to the presence of a scrotum black papule for 20 months. Wide local excision was performed, and histology revealed a malignant melanoma. Chest and abdominal CT revealed metastatic disease, so chemotherapy, immunotherapy and radiotherapy were administered. We describe the evolution over 1 year in this unusual location, as well as complications and the currently available therapeutic options to cure this disease.
Long-Term Real-Life Efficacy and Safety of Repeated Ozurdex® Injections and Factors Associated with Macular Edema Resolution after Retinal Vein Occlusion: The REMIDO 2 Study
Authors: Pommier Stéphan , Meyer Franck , Guigou Sébastien , Barthelemy Tony , Gobert Fréderic , Hajjar Christian , Merite Pierre-Yves , Parrat Eric , Rouhette Hervé , Matonti Fréderic
Published: December 2016

Purpose: The objective was to study long-term real-life efficacy and safety of repeated Ozurdex® injections and factors associated with macular edema (ME) resolution after retinal vein occlusion. Methods: Patients with ME after retinal vein occlusion, receiving Ozurdex®, were included to assess the following endpoints: visual acuity and central retinal thickness, retreatment, number of injections, side effects and ME resolution. ME resolution was defined as permanent discontinuation of intravitreal injections (IVTs) for at least 6 months after the last IVT. Results: A total of 94 eyes were included with an average of 2.6 IVTs (max 6 IVTs). 58.6% of patients gained ≥15 letters and 51.1% of patients showed ME resolution. Among the resolved patients, 64.6% were treatment-naïve, 60.4% had branch retinal vein occlusion, and 78.1% gained ≥15 letters. Conclusions: Ozurdex® seemed to be a valuable treatment which may allow to achieve long-term anatomical and functional improvements and ME resolution with a minimum number of IVTs.
Esophageal Endosonography for the Diagnosis of Intrapulmonary Tumors: A Systematic Review and Meta-Analysis
Authors: Korevaar Daniël A. , Colella Sara , Spijker René , Bossuyt Patrick M. , Konge Lars , Clementsen Paul Frost , Annema Jouke T.
Published: December 2016

Background: Biopsy-based diagnosis in patients with paraesophageal intrapulmonary tumors suspected of lung cancer is crucial for adequate treatment planning. Objective: To evaluate the performance of transesophageal endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) in the diagnosis of intrapulmonary tumors located near or adjacent to the esophagus. Methods: We performed a systematic review (PROSPERO, CRD42016033737) and searched MEDLINE, Embase, BIOSIS Previews, and Web of Science on September 22, 2016, without date or language restrictions. We included studies that evaluated the yield and/or sensitivity of EUS-FNA for diagnosing intrapulmonary tumors. Yield was defined as the number of patients in whom EUS-FNA made a biopsy-proven diagnosis (malignant or nonmalignant) relative to the total number of patients on whom EUS-FNA was performed. Sensitivity was defined as the number of patients in whom EUS-FNA made a biopsy-proven diagnosis of malignancy relative to the total number of patients in whom the tumor was found to be malignant. We performed a random-effects meta-analysis. Results: Of 3,320 search results, 11 studies were included. Ten had a high risk of bias. The total number of patients was 313; the proportion of patients with malignancy ranged from 87 to 100% across these studies. The average yield was 0.90 (95% CI 0.82-0.95) and the average sensitivity was 0.92 (0.83-0.96). In the subgroup of prospective studies (n = 3), the average yield was 0.80 (0.56-0.93) and the average sensitivity was 0.83 (0.58-0.95). EUS-FNA-induced complications were reported for 5/256 patients (2.0%) for whom this information was available. Conclusions: Although the number of high-quality studies is limited, these findings suggest that EUS-FNA is safe and has a high yield for diagnosing intrapulmonary tumors.