bluuebunny/crossref_metadata_2025 huggingface dataset:
Primary lung cancer in children and adolescents: Analysis of a surveillance, epidemiology, and end results database
Authors: Shao Weipeng , Liu Jie , Li Bobo , Guo Xiaokang , Sun Jian , Li Hui , Guo Hongbo
Published: April 2023

Background

The incidence of primary lung cancer (LC) in children and adolescence was rare. We analyzed data from a SEER database to better define the incidence, clinical characters, pathology, treatment, and outcomes of rare primary malignant pulmonary tumors in childhood and adolescence.

Methods

Patients were chosen from the SEER database (SEER*Stat 8.4.0 software) from 2000 to 2019 and all patients were pathologically diagnosed with primary malignant tumors of the lung and bronchus. Demographic characteristics of patients (age, gender, race, primary site, laterality, location, differentiation grade, operation methods, histology, and history of radiotherapy and chemotherapy), as well as TNM stage and survival time, were collected.

Results

A total of 301 cases of children ≤19 years of age with a primary malignant pulmonary tumor were reported to the SEER database from 2000 to 2019. There were 143 men (47.5%) and 158 women (52.5%). Whites represented majority of patients (79.7%), followed by Black (13.6%) and others (6.7%). As for the primary site, the main site was the lower lobe (33.2%), followed by the upper lobe (26.9%). Most of the patients (80.4%) underwent surgery. Lobectomy (39.9%) is the main operation method. Only 28 (9.3%) patients received radiotherapy and 112 (37.2) patients received chemotherapy. Carcinoid tumor was the most common histology (29.6%), followed by pulmonary blastoma (PB) (22.3%), mucoepidermoid carcinoma (MEC) (12.3%), adenocarcinoma (10.3%), neuroendocrine tumor (NET) (5.7%), squamous cell carcinoma (SCC) (5.3%), atypical carcinoma (2.3%). The mean follow-up time was 100 months. For the entire group of children and adolescents, the 1-year OS was 89.1%, and the 3-year overall survival (OS) was 79.7%. the 5-year OS was 77.9%, the 10-year OS was 75.7%, and the 15-year OS was 73.9%. And 1-year lung cancer specificity survival (LCSS) was 89.8%, and the 3-year LCSS was 80.4%. the 5-year LCSS was 79.4%, the 10-year LCSS was 77.7%, and the 15-year LCSS was 75.9%. The OS of atypical carcinoma, carcinoid tumor, and MEC were in the top three.

Conclusions

Primary LC in children and adolescent were rare and histopathological diverse. Fortunately, children and adolescents with LC had an overall favorable outcome after treatment. Histology, differentiation grade, surgery, TNM stage, and therapeutic modalities have important influence on OS. The further treatment experience of each pathological type would make better evidence-based practice possible.
The therapeutic effect of PEI-Fe3O4/pYr-ads-8-5HRE-cfosp-IFNG albumin nanospheres combined with magnetic fluid hyperthermia on hepatoma
Authors: Zhang Hao , Li Suping , Chen Fei , Ma Xingming , Liu Mingying
Published: April 2023

Background

Hepatocellular carcinoma (HCC) is one of the most prevalent and deadly malignant tumors with serious clinical and socioeconomic consequences. Although gene therapy holds great promise in the treatment of hepatoma, its clinical applications are hindered by uncontrolled gene transmission and transcription.

Methods

The pY-ads-8-5HRE-cfosp-IFNG plasmid was constructed and identified by double enzyme digestion and gene sequencing. The expression of pYr-ads-8-5HRE-cfosp-IFNG in HepG2 cells was detected by quantitative PCR. PEI-Fe3O4/pYr-ads-8-5HRE-cfosp-IFNG albumin nanospheres were prepared and characterized. In vitro heating test of magnetic albumin nanospheres in an alternating magnetic field (AMF) was carried out. The therapeutic effect of PEI-Fe3O4/pYr-ads-8-5HRE-cfosp-IFNG albumin nanospheres on hepatocellular carcinoma was investigated by cell and animal experiments. After treatment, mice blood was collected for clinical biochemical analysis and histopathological evaluation of major organs was performed to assess potential adverse effects of treatment.

Results

Double enzyme digestion and gene sequencing showed that the pY-ads-8-5HRE-cfosp-IFNG plasmid was constructed successfully. QPCR results showed that the IFNγ transcript level in the PEI-Fe3O4/pYr-ads-8-5HRE-cfosp-IFNG group was higher than that in the PEI-Fe3O4/pYr-ads-8-cfosp-IFNG group after being treated with hypoxia (P<0.05). TEM revealed that the self-prepared PEI-Fe3O4/pYr-ads-8-5HRE-cfosp-IFNG albumin nanospheres exhibit an approximately spherical or elliptical shape. The hydrodynamic size of the albumin nanospheres was 139.7 nm. The maximum temperature of 0.25 mg/mL solution is stable at about 44°C, which is suitable for tumor thermal therapy without damaging normal tissues. The relative cell inhibition rate of the radiation-gene therapy and MFH combination group was higher than that of other control groups in CCK8 experiment. (P<0.05) Flow cytometry showed that the apoptosis rate and necrosis rate of the combined treatment group were 42.32% and 35.73%, respectively, higher than those of the other groups. (P<0.05) In animal experiments, the mass and volume inhibition rates of the combined treatment group were 66.67% and 72.53%, respectively, higher than those of other control groups. (P<0.05) Clinical biochemical analysis and histopathological evaluation showed no abnormality.

Conclusions

The results indicated the successful construction of the radiation-induced plasmid and demonstrated that the hypoxia enhancer could augment the expression of INFγ in a hypoxia environment. Gene therapy combined with magnetic fluid hyperthermia (MFH) has exhibited excellent outcomes in both cell and animal studies. Our experiments demonstrated that the PEI-Fe3O4/pYr-ads-8-5HRE-cfosp-IFNG albumin nanospheres system is a comprehensive treatment method for hepatoma, which can effectively combine immune genre therapy with hyperthermia.
Radiation-induced bystander effect and its clinical implications
Authors: Tang Haoyi , Cai Luwei , He Xiangyang , Niu Zihe , Huang Haitong , Hu Wentao , Bian Huahui , Huang Hao
Published: April 2023

For many years, targeted DNA damage caused by radiation has been considered the main cause of various biological effects. Based on this paradigm, any small amount of radiation is harmful to the organism. Epidemiological studies of Japanese atomic bomb survivors have proposed the linear-non-threshold model as the dominant standard in the field of radiation protection. However, there is increasing evidence that the linear-non-threshold model is not fully applicable to the biological effects caused by low dose radiation, and theories related to low dose radiation require further investigation. In addition to the cell damage caused by direct exposure, non-targeted effects, which are sometimes referred to as bystander effects, abscopal effects, genetic instability, etc., are another kind of significant effect related to low dose radiation. An understanding of this phenomenon is crucial for both basic biomedical research and clinical application. This article reviews recent studies on the bystander effect and summarizes the key findings in the field. Additionally, it offers a cross-sectional comparison of bystander effects caused by various radiation sources in different cell types, as well as an in-depth analysis of studies on the potential biological mechanisms of bystander effects. This review aims to present valuable information and provide new insights on the bystander effect to enlighten both radiobiologists and clinical radiologists searching for new ways to improve clinical treatments.
Animal models of cancer metastasis to the bone
Authors: Yu Yihan , Li Kanglu , Peng Yizhong , Wu Wei , Chen Fengxia , Shao Zengwu , Zhang Zhicai
Published: April 2023

Cancer metastasis is a major cause of mortality from several tumors, including those of the breast, prostate, and the thyroid gland. Since bone tissue is one of the most common sites of metastasis, the treatment of bone metastases is crucial for the cure of cancer. Hence, disease models must be developed to understand the process of bone metastasis in order to devise therapies for it. Several translational models of different bone metastatic tumors have been developed, including animal models, cell line injection models, bone implant models, and patient-derived xenograft models. However, a compendium on different bone metastatic cancers is currently not available. Here, we have compiled several animal models derived from current experiments on bone metastasis, mostly involving breast and prostate cancer, to improve the development of preclinical models and promote the treatment of bone metastasis.
By using machine learning and in vitro testing, SERPINH1 functions as a novel tumorigenic and immunogenic gene and predicts immunotherapy response in osteosarcoma
Authors: Xia Guang , Wu Song , Luo Ke , Cui Xiaoyu
Published: April 2023

Introduction

The most prevalent bone tumor with a relatively high level of aggressiveness and malignancy is osteosarcoma. The characteristics of the serpin family in osteosarcoma have not been defined.

Methods

In this study, the predictive significance of the serpin superfamily was investigated in the osteosarcoma and Gene Expression Omnibus (GEO) databases from The Cancer Genome Atlas (TCGA).

Results

It was discovered that SERPINH1 is a significant biological marker in osteosarcoma. According to the CCK-8, EdU, and Transwell assays as well as the IHC assay, SERPINH1 may promote osteosarcoma proliferation and migration. It is also more expressed in tumor samples than in healthy samples. SERPINH1 might forecast the effects of immunotherapy. Additionally, immune cells are interacted with through checkpoint, cytokine, and growth factor pathways in osteosarcomas with high SERPINH1 levels. The biological function, immunological characteristics, and treatment response (immunotherapy and chemotherapy responses) of patients with osteosarcoma were successfully predicted using a model related to SERPINH1. SERPINH1 and the SERPINH1-related score predict ferroptosis/pyroptosis/apoptosis/necroptosis in osteosarcoma.

Discussion

The SERPINH1-related score was an effective method for identifying osteosarcoma patients who would respond to immunotherapy and chemotherapy, as well as for predicting the survival outcomes of such patients.
1,25-Dihydroxyvitamin D inhibits hepatic diacyglycerol accumulation and ameliorates metabolic dysfunction in polycystic ovary syndrome rat models
Authors: Yuan Xin , Yang Jianshu , Sun Danlin , Luo Kaiming , Jiang Xiaohong , Wang Long , Xiang Shoukui , Jiang Yijie , Ge Kele , Zhou Zhiyang , Li Bowen , Hua Fei
Published: April 2023

Introduction: We aimed to evaluate the influence of 1,25-dihydroxyvitamin D (1,25(OH)2D) on metabolic dysfunction and elucidate its underlying mechanism using a rat model of polycystic ovary syndrome (PCOS).

Methods: Twenty-four Sprague-Dawley rats were randomly divided into four groups: control group (CON, 2 ml/kg of oral 0.5% CMC), 1,25VD group (oral 0.5% CMC and 2.5 ug/kg intraperitoneal 1,25(OH)2D), PCOS group (1 mg/kg oral letrozole), PCOS+1,25VD group (1 mg/kg oral letrozole orally 2.5 ug/kg intraperitoneal 1,25(OH)2D). The treatments were administered for 8 weeks. Body weight, estrus cycle, insulin tolerance, and oral glucose tolerance of the rats in the different groups were assessed. The rats were euthanized at the 8th weeks, and plasma, ovarian, and liver samples were collected and analyzed. The hepatic lipid profile was characterized using HPLC/MRM.

Results: Letrozole-induced PCOS rats exhibited increased weight, insulin resistance, postprandial glucose abnormalities, and dyslipidemia. Compared with the PCOS group rats, the PCOS+1,25VD group rats showed reduced body weight, increased sensitivity to insulin, decreased postprandial glucose, and elevated levels of high-density lipoprotein cholesterol. Moreover, abnormally increased liver concentrations of total diacylglycerol (DG) and DG species in the PCOS rats were reversed by treatment with 1,25(OH)2D. Additionally, hepatic DG and insulin sensitivity were correlated.

Conclusion: 1,25(OH)2D inhibited hepatic DG accumulation and ameliorated metabolic dysfunction in PCOS rat models.
Fufang shenhua tablet, astragali radix and its active component astragaloside IV: Research progress on anti-inflammatory and immunomodulatory mechanisms in the kidney
Authors: Li Run , Shi Chunru , Wei Cuiting , Wang Chao , Du Hongjian , Hong Quan , Chen Xiangmei
Published: April 2023

Background: Given the limited treatment options available for kidney disease, a significant number of patients turn to alternative therapies, including traditional Chinese medicine. Among these therapies, the Fufang Shenhua tablet (SHT) has garnered attention for its effectiveness in addressing the most common deficiency of Qi and Yin in chronic glomerulonephritis. Notably, the sovereign drug of SHT is Astragali Radix (AR), with the most abundant and effective component being Astragaloside IV (AS-IV). AS-IV has been shown to possess anti-inflammatory and immunomodulatory properties, and it is extensively used in treating kidney diseases. Nevertheless, the molecular mechanisms underlying its action are numerous and intricate, and a comprehensive understanding is yet to be achieved.

Aim of the review: Thus, we have thoroughly examined the existing research and outlined the advancements made in investigating the anti-inflammatory and immunomodulatory mechanisms of SHT, AR and its active component AS-IV, in relation to kidney health. This serves as a dependable foundation for conducting more comprehensive investigations, evaluating efficacy, and making further improvements in the future.

Materials and methods: We conducted a comprehensive literature search utilizing multiple globally recognized databases, including Web of Science, Google Scholar, PubMed, ScienceDirect, Wiley, ACS, Springer, and CNKI. The search keywords used in this study were “Fufang Shenhua tablet,” “Astragali Radix,” “Astragaloside IV,” and “Anti-inflammatory” or “Immunity.”

Results: The mechanism of inflammation inhibition by SHT, AR and its active component AS-IV is mainly related to the signaling pathways such as NF-κB, TLRs, PI3K/AKT, Wnt/β-catenin, and JAK-STAT. Immunomodulation exerts not only activating, stimulating, and regulating effects on macrophages and dendritic cells, but also on immune organs, T-lymphocytes, B-lymphocytes, and a myriad of cytokines. Moreover, the SHT, AR and its active component AS-IV also demonstrate regulatory effects on renal cells, including glomerular mesangial cells, tubular epithelial cells, and podocytes.

Conclusion: To summarize, SHT, AR and its active component AS-IV, exhibit notable therapeutic effects in kidney-related ailments, and their molecular mechanisms for anti-inflammatory and immunomodulatory effects have been extensively explored. However, further standard clinical trials are necessary to evaluate their safety and efficacy in the adjunctive treatment of kidney-related diseases. Moreover, in-depth studies of unverified chemical components and regulatory mechanisms in SHT are required. It is our belief that with continued research, SHT, AR and its active component AS-IV are poised to pave the way for enhancing therapeutic outcomes in kidney-related ailments.
Clinical, economic and humanistic outcomes of medication therapy management services: A systematic review and meta-analysis
Authors: Deng Zhi-Jie , Gui Lin , Chen Jing , Peng Shun-Shun , Ding Yu-Feng , Wei An-Hua
Published: April 2023

Background:Medication therapy management (MTM) services is a method that can effectively improve patients’ conditions, but the efficacy of economic and humanistic outcomes remain unclear. This systematic review and meta-analysis aim to use economic, clinical and humanistic outcomes to evaluate the multi-benefits of MTM services.

Method:A systematic review and meta-analysis was conducted by retrieving PubMed, EMBASE, the Cochrane Library andClinicalTrial.govfrom the inception to April 2022. There were two reviewers screening the records, extracting the data, and assessing the quality of studies independently.

Results:A total of 81 studies with 60,753 participants were included. MTM services were more effective in clinical outcomes with decreasing the rate of readmission (OR: 0.78; 95% CI: 0.73 to 0.83; I2= 56%), emergency department visit (OR: 0.88; 95% CI: 0.81 to 0.96; I2= 32%), adverse drug events (All-cause: OR: 0.68; 95% CI: 0.56 to 0.84; I2= 61%; SAE: OR: 0.51; 95% CI: 0.33 to 0.79; I2= 35%) and drug-related problems (MD: −1.37; 95% CI: −2.24 to −0.5; I2= 95%), reducing the length of stay in hospital (MD: −0.74; 95% CI: −1.37 to −0.13; I2= 70%), while the economic and humanistic outcomes were less effective.

Conclusion:Our systematic review and meta-analysis demonstrated that MTM services had great ability to improve patients’ clinical conditions while the efficacy of economic and humanistic outcomes, with some of the outcomes showing high degree of heterogeneity and possible publication bias, required more future studies to provide stronger evidence.

Systematic Review Registration: [https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=349050], identifier [CRD42022349050].
Sijunzi decoction granules in the prevention and treatment of recurrence of colorectal adenoma: Study protocol for a multicenter, randomized, double-blind, placebo-controlled trial
Authors: Ni Wenjing , Liu Tao , Liu Yujing , Lu Lu , Zhou Bingduo , Dai Yancheng , Zhao Hang , Xu Hanchen , Ji Guang
Published: April 2023

Background: The recurrence of colorectal adenomas (CRAs) after endoscopy predisposes patients to a risk of colorectal cancer. Guided by the traditional Chinese medicine (TCM), patients with colorectal diseases usually manifest with spleen deficiency syndrome (SDS) and are treated with Sijunzi decoction (SJZD). Therefore, this trial aims to explore the efficacy and safety of SJZD in the prevention and treatment of CRAs recurrence.

Methods: SJZD on prevention and treatment of CRAs recurrence after resection: a multicenter, randomized, double-blind, placebo-controlled trial was designed. Patients who undergo polypectomy of CRAs will be recruited and randomized into a SJZD group and a placebo group in a 1:1 ratio. The intervention phase will be 12 months. The follow-up period will last 24 months. The primary outcome is the CRA recurrence rate after intervention. The secondary outcomes include the CRA recurrence rate at the second year post-polypectomy, the pathological type of adenoma and the alterations in SDS scores after intervention.

Discussion: Previous clinical practice has observed the sound effect of SJZD in the context of gastrointestinal diseases. A number of experiments have also validated the active components in SJZD. This trial aims to provide tangible evidence for the usage of SJZD, hoping to reduce the recurrence of CRAs.
Proteomics research in forest trees: A 2012-2022 update
Authors: Castillejo María Angeles , Pascual Jesús , Jorrín-Novo Jesus V. , Balbuena Tiago Santana
Published: April 2023

This review is a compilation of proteomic studies on forest tree species published in the last decade (2012-2022), mostly focused on the most investigated species, including Eucalyptus, Pinus, and Quercus. Improvements in equipment, platforms, and methods in addition to the increasing availability of genomic data have favored the biological knowledge of these species at the molecular, organismal, and community levels. Integration of proteomics with physiological, biochemical and other large-scale omics in the direction of the Systems Biology, will provide a comprehensive understanding of different biological processes, from growth and development to responses to biotic and abiotic stresses. As main issue we envisage that proteomics in long-living plants will thrive light on the plant responses and resilience to global climate change, contributing to climate mitigation strategies and molecular breeding programs. Proteomics not only will provide a molecular knowledge of the mechanisms of resilience to either biotic or abiotic stresses, but also will allow the identification on key gene products and its interaction. Proteomics research has also a translational character being applied to the characterization of the variability and biodiversity, as well as to wood and non-wood derived products, traceability, allergen and bioactive peptides identification, among others. Even thought, the full potential of proteomics is far from being fully exploited in forest tree research, with PTMs and interactomics being reserved to plant model systems. The most outstanding achievements in forest tree proteomics in the last decade as well as prospects are discussed.
Comprehensive genomic identification of cotton starch synthase genes reveals that GhSS9 regulates drought tolerance
Authors: Dai Maohua , Yang Xiaomin , Chen Quanjia , Bai Zhigang
Published: April 2023

Introduction

Starch metabolism is involved in the stress response. Starch synthase (SS) is the key enzyme in plant starch synthesis, which plays an indispensable role in the conversion of pyrophosphoric acid to starch. However, the SS gene family in cotton has not been comprehensively identified and systematically analyzed.

Result

In our study, a total of 76 SS genes were identified from four cotton genomes and divided into five subfamilies through phylogenetic analysis. Genetic structure analysis proved that SS genes from the same subfamily had similar genetic structure and conserved sequences. A cis-element analysis of the SS gene promoter showed that it mainly contains light response elements, plant hormone response elements, and abiotic stress elements, which indicated that the SS gene played key roles not only in starch synthesis but also in abiotic stress response. Furthermore, we also conducted a gene interaction network for SS proteins. Silencing GhSS9 expression decreased the resistance of cotton to drought stress. These findings suggested that SS genes could be related to drought stress in cotton, which provided theoretical support for further research on the regulation mechanism of SS genes on abiotic starch synthesis and sugar levels.
Childhood trauma and problematic internet use: A meta-analysis based on students in mainland China
Authors: Wang Xiaotong , Li Dexian , Li Shunyu
Published: April 2023

Background

In recent years, the relationship between childhood trauma and problematic Internet use has been widely studied by scholars, but the research conclusions on the relationship between them are not consistent. Some studies report that childhood trauma and problematic Internet use are significantly correlated. However, others believe that there is a weak correlation between them. So the relationship between them needs to be studied further. The aim of this study is to explore the relationship between childhood trauma and problematic Internet use, and the effects of some moderating variables on both for students in Mainland China.

Methods

This study followed the requirements of Preferred Reporting Items for Systematic Review and Meta-analyses (PRISMA) statement for literature screening. We searched the related studies on childhood trauma and problematic Internet use in Mainland China from January 2009 to November 2022 from CNKI, Wanfang Data, Chongqing VIP Information Co., Ltd. (VIP), Baidu scholar, ProQuest dissertations, SAGE Online Journals, Elsevier SDOL, Taylor & Francis, Springer, Web of Science, Google Scholar, EBSCO, Medline, Scopus Database, PubMed Central, Embase, The Cochrane Library, PsycINFO, CMA 3.0 was used to analyse the overall effect and test the moderating effect.

Results

Among the papers included in the meta-analysis in this study, 31 papers reflected the relationship between childhood trauma and problematic Internet use, involving 52,503 subjects, and the sample size ranged from 388 to 16,130. This relationship between childhood trauma and problematic Internet use can be considered as a moderate correlation (r = 0.281, 95%CI[0.221, 0.338], p < 0.001). The results showed that the relationship between childhood trauma and problematic Internet use was affected by different problematic Internet use measures. Meanwhile, meta-regression demonstrated that the relationship between childhood trauma and problematic Internet use was moderated by survey’s year. Specifically, the correlation coefficient between childhood trauma and problematic Internet use also increases with increasing year. However, the relationship between childhood trauma and problematic Internet use was not affected by the region, grade, childhood trauma measures, publication source.

Conclusion

Childhood trauma is closely related to problematic Internet use. In order to reduce problematic Internet use, corresponding prevention and intervention measures should be taken.
Relational bullying and disordered eating: Testing a moderated mediation model of the role of shame and self-compassion
Authors: Bellows Lindsay A. , Couturier Laura E. , Dunn Leigh C. , Carter Jacqueline C.
Published: April 2023

Objective

Experiences of relational bullying (RB) in adolescence are associated with the development of disordered eating. This association may be related to heightened shame resulting from perceived social inferiority, low social rank, and/or negative evaluation by others. Self-compassion may act as a protective factor against the influence of RB on shame and disordered eating. In the current study, we investigated whether shame mediated the relationship between recalled RB and current disordered eating in a sample of young adults. Then, using conditional process analysis, we examined whether the observed mediation was moderated by self-compassion.

Method

Participants were 359 young adults (aged 17–25) who completed online self-report measures of recalled RB experiences and current disordered eating, shame, and self-compassion.

Results

Experiences of RB were positively related to current shame and disordered eating, and negatively related to current self-compassion, with small-to-medium effect sizes. The association between RB and disordered eating was partially mediated by shame, and this mediation was moderated by self-compassion.

Discussion

Our results suggest that young adults with lower self-compassion are more likely to demonstrate a relationship between recalled RB and disordered eating through the mechanism of shame. These findings have important implications for both anti-bullying awareness and eating disorder prevention programs.
Psychedelic-induced mystical experiences: An interdisciplinary discussion and critique
Authors: Mosurinjohn Sharday , Roseman Leor , Girn Manesh
Published: April 2023

Contemporary research on serotonergic psychedelic compounds has been rife with references to so-called ‘mystical’ subjective effects. Several psychometric assessments have been used to assess such effects, and clinical studies have found quantitative associations between ‘mystical experiences’ and positive mental health outcomes. The nascent study of psychedelic-induced mystical experiences, however, has only minimally intersected with relevant contemporary scholarship from disciplines within the social sciences and humanities, such as religious studies and anthropology. Viewed from the perspective of these disciplines—which feature rich historical and cultural literatures on mysticism, religion, and related topics—‘mysticism’ as used in psychedelic research is fraught with limitations and intrinsic biases that are seldom acknowledged. Most notably, existing operationalizations of mystical experiences in psychedelic science fail to historicize the concept and therefore fail to acknowledge its perennialist and specifically Christian bias. Here, we trace the historical genesis of the mystical in psychedelic research in order to illuminate such biases, and also offer suggestions toward more nuanced and culturally-sensitive operationalizations of this phenomenon. In addition, we argue for the value of, and outline, complementary ‘non-mystical’ approaches to understanding putative mystical-type phenomena that may help facilitate empirical investigation and create linkages to existing neuro-psychological constructs. It is our hope that the present paper helps build interdisciplinary bridges that motivate fruitful paths toward stronger theoretical and empirical approaches in the study of psychedelic-induced mystical experiences.
Spatial segregation of families with migrant background in the high-status City of Munich: How strong is the effect of socio-economic status?
Authors: Hanslmaier Michael , Teltemann Janna , Windzio Michael
Published: April 2023

Ethnic residential segregation can result from preferential choices or from market forces. Depending on whether it evolved voluntarily or forcibly, segregation can have differential effects on immigrant integration. Using the example of Munich as a major German city, we examine the unequal spatial distribution of migrants and non-migrants. Following an approach proposed by Frank Kalter we calculate indices of residential segregation, which are adjusted for differences in socio-economic status. Results show that almost 14 percent of the residential segregation of immigrants can be attributed to socio-economic restrictions. This finding suggests that factors related to immigration and, possibly, also ethnic boundaries are determinants of the unequal spatial distribution also in high-status cities such as Munich.
Testing the key processes that accelerate outer radiation belt relativistic electrons during geomagnetic storms
Authors: Hua Man , Bortnik Jacob , Spence Harlan E. , Reeves Geoffrey D.
Published: April 2023

Since the discovery of the Earth’s radiation belts in 1958, it has always been a challenge to determine the dominant physical mechanisms, whether local acceleration by chorus or inward radial diffusion, that leads to outer radiation belt relativistic electron flux enhancements. In this study, we test a chain of processes with several potential successive steps that is believed to accelerate outer belt relativistic electrons. By performing correlation analysis of different part of this chain, including the geomagnetic condition, evolution of source and seed electron fluxes, chorus wave activity, and maximum fluxes (jmax) of relativistic electrons, we aim to identify the critical steps that lead to acceleration of MeV electrons. Based on 5-years of Van Allen Probes observations, our results confirm the repeatable response of both source and seed electrons to the storms, showing a significant flux enhancement during the main phase of storms, followed by either a gradual decay or flux persistence at a stable level. However, it is the intense and prolonged occurrence of substorms that contributes to the long-lasting existence of both source and seed electrons, which is also strongly associated with the jmax of relativistic electrons. The significant correlation (Correlation Coefficient, CC∼0.8) between the seed electron fluxes and jmax reveal that the prolonged and pronounced seed electrons are the prerequisite for the significant flux enhancement of relativistic electrons regardless of the acceleration mechanism. The slightly smaller CC (∼0.5–0.7) between source electron fluxes and jmax of relativistic electrons indicates that while local acceleration by chorus wave plays an important role to accelerate relativistic electrons to jmax, other mechanisms such as inward radial diffusion are still needed in this process. The CC between the source electrons and the chorus wave amplitude increases with increasing levels of substorms, showing (CC)max of ∼0.8, which further supports the crucial role of chorus waves in accelerating the relativistic electrons during intense substroms.
Protective role of remote ischemic conditioning in renal transplantation and partial nephrectomy: A systematic review and meta-analysis of randomized controlled trials
Authors: Zhang Wenfu , Wu Yingting , Zeng Mingwang , Yang Chao , Qiu Zhengang , Liu Rongrong , Wang Lifeng , Zhong Maolin , Chen Qiaoling , Liang Weidong
Published: April 2023

Objective

Studies have shown that remote ischemic conditioning (RIC) can effectively attenuate ischemic-reperfusion injury in the heart and brain, but the effect on ischemic-reperfusion injury in patients with kidney transplantation or partial nephrectomy remains controversial. The main objective of this systematic review and meta-analysis was to investigate whether RIC provides renal protection after renal ischemia-reperfusion injury in patients undergoing kidney transplantation or partial nephrectomy.

Methods

A computer-based search was conducted to retrieve relevant publications from the PubMed database, Embase database, Cochrane Library and Web of Science database. We then conducted a systematic review and meta-analysis of randomized controlled trials that met our study inclusion criteria.

Results

Eleven eligible studies included a total of 1,145 patients with kidney transplantation or partial nephrectomy for systematic review and meta-analysis, among whom 576 patients were randomly assigned to the RIC group and the remaining 569 to the control group. The 3-month estimated glomerular filtration rate (eGFR) was improved in the RIC group, which was statistically significant between the two groups on kidney transplantation [P < 0.001; mean difference (MD) = 2.74, confidence interval (CI): 1.41 to 4.06; I2 = 14%], and the 1- and 2-day postoperative Scr levels in the RIC group decreased, which was statistically significant between the two groups on kidney transplantation (1-day postoperative: P < 0.001; MD = 0.10, CI: 0.05 to 0.15, I2 = 0; 2-day postoperative: P = 0.006; MD = 0.41, CI: 0.12 to 0.70, I2 = 0), but at other times, there was no significant difference between the two groups in Scr levels. The incidence of delayed graft function (DGF) decreased, but there was no significant difference (P = 0.60; 95% CI: 0.67 to 1.26). There was no significant difference between the two groups in terms of cross-clamp time, cold ischemia time, warm ischemic time, acute rejection (AR), graft loss or length of hospital stay.

Conclusion

Our meta-analysis showed that the effect of remote ischemia conditioning on reducing serum creatinine (Scr) and improving estimate glomerular filtration rate (eGFR) seemed to be very weak, and we did not observe a significant protective effect of RIC on renal ischemic-reperfusion. Due to small sample sizes, more studies using stricter inclusion criteria are needed to elucidate the nephroprotective effect of RIC in renal surgery in the future.
Empirical Research on Early Internationalization of Firms in Sufficiently-Sized Domestic Market Country
Authors: Otomo Saki , Ishida Shuichi , Yang-Yoshihara Mariko
Published: April 2023

Early internationalization and success in foreign markets play an important role in both a firm’s growth and its impact on the global economy. We conducted a study on Japanese high-tech startups to investigate the factors that derive early internationalization in firms founded in countries with a large domestic market, despite the absence of strong incentives to operate overseas. Quantitative data were collected from 71 startups and analyzed with PLS-SEM (Partial least squares path modeling). Our result showed that the factors we extracted from the previous studies on the internationalization process in small-size markets would also apply in countries with large domestic markets. In addition, considerations and the types of technology, which we extracted from qualitative research, verified the effect. According to our mediator analysis, an entrepreneur’s international orientation explains certain conditions related to a domestic market that affect a firms’ decision to pursue early internationalization. Our study makes contributions at multiple levels, benefiting entrepreneurs who are considering overseas expansion as well as policymakers who aim to promote early internationalization efforts.
Deep Learning in Air Traffic Management (ATM): A Survey on Applications, Opportunities, and Open Challenges
Authors: Pinto Neto Euclides Carlos , Baum Derick Moreira , Almeida Jorge Rady de , Camargo João Batista , Cugnasca Paulo Sergio
Published: April 2023

Currently, the increasing number of daily flights emphasizes the importance of air transportation. Furthermore, Air Traffic Management (ATM) enables air carriers to operate safely and efficiently through the multiple services provided. Advanced analytic solutions have demonstrated the potential to solve complex problems in several domains, and Deep Learning (DL) has attracted attention due to its impressive results and disruptive capabilities. The adoption of DL models in ATM solutions enables new cognitive services that have never been considered before. The main goal of this research is to present a comprehensive review of state-of-the-art Deep Learning (DL) solutions for Air Traffic Management (ATM). This review focuses on describing applications, identifying opportunities, and highlighting open challenges to foster the evolution of ATM systems. To accomplish this, we discuss the fundamental topics of DL and ATM and categorize the contributions based on different approaches. First, works are grouped based on the DL approach adopted. Then, future directions are identified based on the ATM solution area. Finally, open challenges are listed for both DL applications and ATM solutions. This article aims to support the community by identifying research problems to be faced in the future.
Developing a Crop Water Production Function for Alfalfa under Deficit Irrigation: A Case Study in Eastern Colorado
Authors: Sitterson Jan M. , Andales Allan A. , Mooney Daniel F. , Capurro Maria Cristina , Brummer Joe E.
Published: April 2023

Recent Colorado, USA water law provisions allow a portion of irrigation water to be leased between agricultural and other users. Reducing consumptive use (CU) through deficit irrigation while maintaining some crop production could allow farmers to earn revenue from leasing water rights. This observational study aimed to determine if deficit irrigation of alfalfa (Medicago sativa L.) can be used to reduce CU, provide parameters for an alfalfa crop water production function (WPF), and evaluate the potential for improved farm income by leasing water. Soil water balance, evapotranspiration (ET), and dry matter yield from eight commercial fields (1.70 to 2.14 ha zones), growing subsurface drip-irrigated alfalfa, were monitored for five seasons (2018–2022) at Kersey, Colorado. Four irrigation treatments [Standard Irrigation (SI) = irrigate when soil water deficit (D) exceeds management allowed depletion (MAD); Moderate Deficit Irrigation (MDI) = 70% of SI; Severe Deficit Irrigation (SDI) = 50% of SI; and Over Irrigation (OI) = 120% of SI] were applied, with two zones per treatment. Reductions in CU ranged from 205 to 260 mm per season. The shape of the alfalfa WPF (dry biomass yield vs. ET) was concave, indicating that water use efficiency (WUE) could be optimized through deficit irrigation. The average WUE was 0.17 Mg ha−1 cm−1 and tended to increase with greater deficits. Deficit irrigation also increased the relative feed value. If conserved CU from deficit irrigation can be leased into a transfer water market, farmers could profit when the water lease revenue exceeds the forgone profit from alfalfa production. We found incremental profit from deficit irrigation and water leasing to be positive, assuming 2020 prices for hay ($230 bale−1) and water prices above $0.50 m−3.
A Framework Combining CENTURY Modeling and Chronosequences Sampling to Estimate Soil Organic Carbon Stock in an Agricultural Region with Large Land Use Change
Authors: Liu Xiaoyu , Chen Yin , Liu Yang , Wang Shihang , Jin Jiaming , Zhao Yongcun , Yu Dongsheng
Published: April 2023

Agricultural land use has a remarkable influence on the stock and distribution of soil organic carbon (SOC). However, both regional soil sampling and process-based ecosystem models for SOC estimation at the regional scale have limitations when applied in areas with a large land use change. In the present study, a framework (CMCS) combining CENTURY modeling (CM) and chronosequences sampling (CS) was established, and a case study was conducted in Cangshan County, where vegetable cultivation conversion from grain production was significant in recent decades. The SOC stock (SOCS) of the non-vegetable area estimated by CM was comparable to that estimated by regional soil sampling in 2008. This result confirmed that CM was reliable in modeling SOC dynamics in a non-vegetable area without land use change. However, when applied to the overall cropland of Cangshan County, the CM, without considering the land use change, underestimated the SOCS by 0.23 Tg (6%), compared with the observed measurements (3.58 and 3.81 Tg, respectively). Using the CMCS framework of our study, the underestimation of CM was offset by the SOC sequestration estimated by CS. The SOCS estimated by the CMCS framework ranged from 3.72 to 4.02 Tg, demonstrating that this framework is reliable for the regional SOC estimation of large-area land use change. In addition, annual SOCS dynamics were obtained by this framework. The CMCS framework provides a low-cost and practicable method for the estimation of the regional SOC dynamic, which can further support the strategy of carbon peaking and carbon neutrality in China.
Effect of Branch Bending on the Canopy Characteristics and Growth of Peach (Prunus persica (L.) Batsch)
Authors: Zhang Binbin , Zheng Fengshi , Geng Wenwen , Du Hao , Xiao Yuansong , Peng Futian
Published: April 2023

A reasonable main branch opening angle can improve the canopy light environment of a tree, change the direction of nutrient transport, and promote the formation of flower buds. In this experiment, 3-year-old ‘Lu Hong 618’ was used as the test material to study the effects of different main branch opening angles (three treatments: 45°, 65°, and 85°) on the canopy light distribution, canopy characteristics, fruit quality, and flower bud quality of ‘Y’-shaped peach trees. The main findings were as follows: the solar energy utilization in the canopy varied greatly between different main branch opening angles, with the best relative light intensity of the canopy at 85°. In addition, the canopy light distribution on photosynthesis was more reasonable at 85°, and the daily variation range of CO2 concentration in the inner canopy was the greatest, which facilitated ventilation and light penetration in the inner canopy. With the increase in the main branch opening angle, the new growth of peach trees and the growth of the main branch trunk were inhibited. A reasonable branch opening angle results in good branch structure and leaf morphology. Leaf stomatal morphology and the soluble sugar and starch contents at different levels of the canopy are influenced by canopy light, and higher levels of canopy light can significantly increase the stomatal size and improve the leaf photosynthetic capacity, SPAD value, and soluble sugar and starch contents. With the increase in the main branch opening angle, C/N tended to increase. In addition, the distribution pattern of fruit quality in the canopy was similar to that of light distribution. In the same fruiting part, the fruit quality was higher at 85° and 45° than at 65° and slightly higher at 85° than at 45°, but the difference was not significant. The effects of different main branch opening angle treatments on the fruit weight, flesh firmness, soluble solids, and titratable acid were more significant. The 85° pulling angle effectively increased the light intensity of the different canopy layers of the peach trees and promoted the accumulation of nutrients. An 85° pulling angle can be used as a more suitable pulling angle for peach tree shaping cultivation.
Microbial Persistence, Replacement and Local Antimicrobial Therapy in Recurrent Bone and Joint Infection
Authors: Young Bernadette C. , Dudareva Maria , Vicentine Margarete P. , Hotchen Andrew J. , Ferguson Jamie , McNally Martin
Published: April 2023

We report microbiological results from a cohort of recurrent bone and joint infection to define the contributions of microbial persistence or replacement. We also investigated for any association between local antibiotic treatment and emerging antimicrobial resistance. Microbiological cultures and antibiotic treatments were reviewed for 125 individuals with recurrent infection (prosthetic joint infection, fracture-related infection, and osteomyelitis) at two UK centres between 2007 and 2021. At re-operation, 48/125 (38.4%) individuals had an organism from the same bacterial species as at their initial operation for infection. In 49/125 (39.2%), only new species were isolated in culture. In 28/125 (22.4%), re-operative cultures were negative. The most commonly persistent species were Staphylococcus aureus (46.3%), coagulase-negative Staphylococci (50.0%), and Pseudomonas aeruginosa (50.0%). Gentamicin non-susceptible organisms were common, identified at index procedure in 51/125 (40.8%) and at re-operation in 40/125 (32%). Gentamicin non-susceptibility at re-operation was not associated with previous local aminoglycoside treatment (21/71 (29.8%) vs. 19/54 (35.2%); p = 0.6). Emergence of new aminoglycoside resistance at recurrence was uncommon and did not differ significantly between those with and without local aminoglycoside treatment (3/71 (4.2%) vs. 4/54 (7.4%); p = 0.7). Culture-based diagnostics identified microbial persistence and replacement at similar rates in patients who re-presented with infection. Treatment for orthopaedic infection with local antibiotics was not associated with the emergence of specific antimicrobial resistance.
Sargassum filipendula, a Source of Bioactive Compounds with Antioxidant and Matrix Metalloproteinases Inhibition Activities In Vitro with Potential Dermocosmetic Application
Authors: Luna-Pérez Yonadys , Ríos-López Lady Giselle , Otero-Tejada Elver Luis , Mejía-Giraldo Juan Camilo , Puertas-Mejía Miguel Ángel
Published: April 2023

The antioxidant and the potential inhibitory capacity of matrix metalloproteinases of the phlorotannin-type polyphenolic and fucoidan-type polysaccharides extracts obtained from the macroalga S. filipendula were evaluated. Through chromatographic and spectroscopic techniques, the corresponding chemical structure of compounds present in the extracts was determined. Antioxidant capacity was evaluated using the methyl linoleate model for the inhibition of lipid peroxidation, and the free radical scavenging capacity was assessed using DPPH, ABTS, •OH, O2•− methods. The matrix metalloproteinase inhibition potential was measured by collagenase and elastase inhibition tests, using epigallocatechin gallate as a positive control. The extracts exhibited a high scavenging capacity of radical species evaluated and inhibition of diene conjugate formation and thiobarbituric acid reactive substances. The results showed that the crude extracts presented dose-dependent collagenase and elastase inhibition, with IC50 values between 0.04 and 1.61 mg/mL. The structure of the residues of the polysaccharide was identified mainly as (1→3)-sulfated (1→3) α-l-fucopyranose at carbon 4 and residues of β-d-glucopyranose, α-d-Mannopyranose, and β-d-Galactopyranose, while in the polyphenol extract the presence of phloroglucinol was identified and the presence of eckol, bifuhalol, and trifuhalol was suggested. Our results allow us to infer that S. filipendula is a potential source of bioactive ingredients with antioxidant and anti-aging activity.