bluuebunny/crossref_metadata_2025 huggingface dataset:
Critical Roles of Tumor Extracellular Vesicles in the Microenvironment of Thoracic Cancers
Authors: Kara-Terki Lyna , Treps Lucas , Blanquart Christophe , Fradin Delphine
Published: August 2020

Extracellular vesicles (EVs), such as exosomes, are critical mediators of intercellular communication between tumor cells and other cells located in the microenvironment but also in more distant sites. Exosomes are small EVs that can carry a variety of molecules, such as lipids, proteins, and non-coding RNA, especially microRNAs (miRNAs). In thoracic cancers, including lung cancers and malignant pleural mesothelioma, EVs contribute to the immune-suppressive tumor microenvironment and to tumor growth and metastasis. In this review, we discuss the recent understanding of how exosomes behave in thoracic cancers and how and why they are promising liquid biomarkers for diagnosis, prognosis, and therapy, with a special focus on exosomal miRNAs.
Blockade of Hemichannels Normalizes the Differentiation Fate of Myoblasts and Features of Skeletal Muscles from Dysferlin-Deficient Mice
Authors: Cea Luis A. , Fernández Gabriela , Arias-Bravo Guisselle , Castillo-Ruiz Mario , Escamilla Rosalba , Brañes María C. , Sáez Juan C.
Published: August 2020

Dysferlinopathies are muscle dystrophies caused by mutations in the gene encoding dysferlin, a relevant protein for membrane repair and trafficking. These diseases are untreatable, possibly due to the poor knowledge of relevant molecular targets. Previously, we have shown that human myofibers from patient biopsies as well as myotubes derived from immortalized human myoblasts carrying a mutated form of dysferlin express connexin proteins, but their relevance in myoblasts fate and function remained unknown. In the present work, we found that numerous myoblasts bearing a mutated dysferlin when induced to acquire myogenic commitment express PPARγ, revealing adipogenic instead of myogenic commitment. These cell cultures presented many mononucleated cells with fat accumulation and within 48 h of differentiation formed fewer multinucleated cells. In contrast, dysferlin deficient myoblasts treated with boldine, a connexin hemichannels blocker, neither expressed PPARγ, nor accumulated fat and formed similar amount of multinucleated cells as wild type precursor cells. We recently demonstrated that myofibers of skeletal muscles from blAJ mice (an animal model of dysferlinopathies) express three connexins (Cx39, Cx43, and Cx45) that form functional hemichannels (HCs) in the sarcolemma. In symptomatic blAJ mice, we now show that eight-week treatment with a daily dose of boldine showed a progressive recovery of motor activity reaching normality. At the end of this treatment, skeletal muscles were comparable to those of wild type mice and presented normal CK activity in serum. Myofibers of boldine-treated blAJ mice also showed strong dysferlin-like immunoreactivity. These findings reveal that muscle dysfunction results from a pathophysiologic mechanism triggered by mutated dysferlin and downstream connexin hemichannels expressed de novo lead to a drastic reduction of myogenesis and favor muscle damage. Thus, boldine could represent a therapeutic opportunity to treat dysfernilopathies.
Transient Receptor Potential Melastatin 2 (TRPM2) Inhibition by Antioxidant, N-Acetyl-l-Cysteine, Reduces Global Cerebral Ischemia-Induced Neuronal Death
Authors: Hong Dae Ki , Kho A Ra , Lee Song Hee , Jeong Jeong Hyun , Kang Beom Seok , Kang Dong Hyeon , Park Min Kyu , Park Kyoung-Ha , Lim Man-Sup , Choi Bo Young , Suh Sang Won
Published: August 2020

A variety of pathogenic mechanisms, such as cytoplasmic calcium/zinc influx, reactive oxygen species production, and ionic imbalance, have been suggested to play a role in cerebral ischemia induced neurodegeneration. During the ischemic state that occurs after stroke or heart attack, it is observed that vesicular zinc can be released into the synaptic cleft, and then translocated into the cytoplasm via various cation channels. Transient receptor potential melastatin 2 (TRPM2) is highly distributed in the central nervous system and has high sensitivity to oxidative damage. Several previous studies have shown that TRPM2 channel activation contributes to neuroinflammation and neurodegeneration cascades. Therefore, we examined whether anti-oxidant treatment, such as with N-acetyl-l-cysteine (NAC), provides neuroprotection via regulation of TRPM2, following global cerebral ischemia (GCI). Experimental animals were then immediately injected with NAC (150 mg/kg/day) for 3 and 7 days, before sacrifice. We demonstrated that NAC administration reduced activation of GCI-induced neuronal death cascades, such as lipid peroxidation, microglia and astroglia activation, free zinc accumulation, and TRPM2 over-activation. Therefore, modulation of the TRPM2 channel can be a potential therapeutic target to prevent ischemia-induced neuronal death.
Developmental Methylome of the Medicinal Plant Catharanthus roseus Unravels the Tissue-Specific Control of the Monoterpene Indole Alkaloid Pathway by DNA Methylation
Authors: Dugé de Bernonville Thomas , Maury Stéphane , Delaunay Alain , Daviaud Christian , Chaparro Cristian , Tost Jörg , O’Connor Sarah Ellen , Courdavault Vincent
Published: August 2020

Catharanthus roseus produces a wide spectrum of monoterpene indole alkaloids (MIAs). MIA biosynthesis requires a tightly coordinated pathway involving more than 30 enzymatic steps that are spatio-temporally and environmentally regulated so that some MIAs specifically accumulate in restricted plant parts. The first regulatory layer involves a complex network of transcription factors from the basic Helix Loop Helix (bHLH) or AP2 families. In the present manuscript, we investigated whether an additional epigenetic layer could control the organ-, developmental- and environmental-specificity of MIA accumulation. We used Whole-Genome Bisulfite Sequencing (WGBS) together with RNA-seq to identify differentially methylated and expressed genes among nine samples reflecting different plant organs and experimental conditions. Tissue specific gene expression was associated with specific methylation signatures depending on cytosine contexts and gene parts. Some genes encoding key enzymatic steps from the MIA pathway were found to be simultaneously differentially expressed and methylated in agreement with the corresponding MIA accumulation. In addition, we found that transcription factors were strikingly concerned by DNA methylation variations. Altogether, our integrative analysis supports an epigenetic regulation of specialized metabolisms in plants and more likely targeting transcription factors which in turn may control the expression of enzyme-encoding genes.
Intratumoral Distribution and pH-Dependent Drug Release of High Molecular Weight HPMA Copolymer Drug Conjugates Strongly Depend on Specific Tumor Substructure and Microenvironment
Authors: Noack Anne-Kathrin , Lucas Henrike , Chytil Petr , Etrych Tomáš , Mäder Karsten , Mueller Thomas
Published: August 2020

Stimulus-sensitive polymer drug conjugates based on high molecular weight N-(2-hydroxypropyl) methacrylamide (HPMA) copolymers carrying doxorubicin via a pH-dependent cleavable bond (pHPMA-Dox) were previously shown to be able to overcome multi-drug resistance. Nevertheless, a tumor type dependent differential response was observed. Although an improved and more selective tumor accumulation of pHPMA-Dox is generally achieved due to the enhanced permeability and retention (EPR) effect, little is known about the fate of these conjugates upon entering the tumor tissue, which could explain the different responses. In this study, we compared in vitro and in vivo accumulation and Dox-activation of pHPMA-Dox in three cancer cell line models (1411HP, A2780cis, HT29) and derived xenograft tumors using a near-infrared fluorescence-labeled pHPMA-Dox conjugate. Firstly, cytotoxicity assays using different pH conditions proved a stepwise, pH-dependent increase in cytotoxic activity and revealed comparable sensitivity among the cell lines. Using multispectral fluorescence microscopy, we were able to track the distribution of drug and polymeric carrier simultaneously on cellular and histological levels. Microscopic analyses of cell monolayers confirmed the assumed mechanism of cell internalization of the whole conjugate followed by intracellular cleavage and nuclear accumulation of Dox in all three cell lines. In contrast, intratumoral distribution and drug release in xenograft tumors were completely different and were associated with different tissue substructures and microenvironments analyzed by Azan- and Hypoxisense®-staining. In 1411HP tumors, large vessels and less hypoxic/acidic microenvironments were associated with a pattern resulting from consistent tissue distribution and cellular uptake as whole conjugate followed by intracellular drug release. In A2780cis tumors, an inconsistent pattern of distribution partly resulting from premature drug release was associated with a more hypoxic/acidic microenvironment, compacted tumor tissue with compressed vessels and specific pre-damaged tissue structures. A completely different distribution pattern was observed in HT29 tumors, resulting from high accumulation of polymer in abundant fibrotic structures, with small embedded vessels featuring this tumor type together with pronounced premature drug release due to the strongly hypoxic/acidic microenvironment. In conclusion, the pattern of intratumoral distribution and drug release strongly depends on the tumor substructure and microenvironment and may result in different degrees of therapeutic efficacy. This reflects the pronounced heterogeneity observed in the clinical application of nanomedicines and can be exploited for the future design of such conjugates.
Adipose Tissue Fibrosis: Mechanisms, Models, and Importance
Authors: DeBari Megan K. , Abbott Rosalyn D.
Published: August 2020

Increases in adipocyte volume and tissue mass due to obesity can result in inflammation, further dysregulation in adipose tissue function, and eventually adipose tissue fibrosis. Like other fibrotic diseases, adipose tissue fibrosis is the accumulation and increased production of extracellular matrix (ECM) proteins. Adipose tissue fibrosis has been linked to decreased insulin sensitivity, poor bariatric surgery outcomes, and difficulty in weight loss. With the rising rates of obesity, it is important to create accurate models for adipose tissue fibrosis to gain mechanistic insights and develop targeted treatments. This article discusses recent research in modeling adipose tissue fibrosis using in vivo and in vitro (2D and 3D) methods with considerations for biomaterial selections. Additionally, this article outlines the importance of adipose tissue in treating other fibrotic diseases and methods used to detect and characterize adipose tissue fibrosis.
Immersive Environment for Occupational Therapy: Pilot Study
Authors: Rodrigues José , Coelho Tiago , Menezes Paulo , Restivo Maria Teresa
Published: August 2020

There has been increasing interest in the application of virtual reality (VR) to occupational therapy and rehabilitation fields. This work presents a VR-based city adventure framework designed for occupational therapy. Its concept is based on taking the user to travel around a virtual city, where the motion is powered by the execution of some type of physical exercise, namely cycling in the current example. This framework is extensible and may integrate different goals focused on improving/maintaining motor and cognitive skills necessary for daily activities, mainly through upper and lower limb exercises and mental challenges. This led to the development of an immersive system, which was tested with an elderly sample (15 participants) in a day center and at a retirement home. Usability, satisfaction, performance and side effects were evaluated to understand future applicability and work directions.
Effects of Various Behaviours in the Break Times between Learning
Authors: Ito Takashi , Takahashi Kenichi
Published: August 2020

E-learning provides users with the experience of learning at their own pace, without being restricted by time and place. Learners tend to get tired gradually during learning, and therefore, it is important to have breaks between studies to maintain learning performance. Furthermore, the break time has to be effective so that learners can refresh. This study compares three types of breaks: quiet, active, and communication breaks. In the experiment, university students in their twenties are asked to solve as many mathematical calculation problems as possible for 15 min. The experiment comprises three learning sessions and two three-minute breaks between them. Learners are assigned one of the three types of breaks to refresh themselves. The effect of those resting behaviours on learning is examined, and the results are evaluated with regard to the accuracy of their calculations and their answering time. The effects of these behaviours will be discussed based on the experimental results and the questionnaire.
Effect of Pheromone-Mediated Mating Disruption on Pest Population Density of Maruca vitrata (Fabricius) (Crambidae: Lepidoptera)
Authors: Dhanyakumar Onkarappa , Srinivasan Ramasamy , Mohan Muthugounder , Venkatesan Thiruvengadam , Murali Mohan Kamanur , Nagesha Narayanappa , Sotelo-Cardona Paola
Published: August 2020

The legume pod borer (Maruca vitrata) is one of the most serious legume pests due to its wide host range and high damage potential. Pheromone components on M. vitrata have been previously identified, allowing research on more environmentally friendly IPM tools for its control. M. vitrata produces a three-component pheromone blend containing (E, E)-10,12-hexadecadienal (major), (E, E)-10,12-hexadecadienol (minor), (E)-10-hexadecenal (minor). This study focused on the efficacy of synthetic pheromone lures and their blend components for mating disruption in M. vitrata. Under laboratory conditions, the mating behavior of M. vitrata pairs was observed from 18:00 to 02:00 h in an interval of 20 min to assess the efficacy of different pheromone lures. The scotophase behavior results show that the complete pheromone blend (E, E)-10,12-hexadecadienal + (E, E)-10,12-hexadecadienol + (E)-10-hexadecenal with a blend ratio of 1:1:1 effectively disrupted mating. The impact on mating disruption was evident from the lower fecundity and egg hatch/eclosion. The same lures were evaluated in a small-scale caged field study. The results show that the pheromone blend of (E, E)-10,12-hexadecadienal + (E, E)-10,12-hexadecadienol + (E)-10-hexadecenal in a1:1:1 ratio significantly disrupted the normal mating, leading to lower flower and pod damage and higher mung bean yield.
Identification of microRNAs Targeting the Transporter Associated with Antigen Processing TAP1 in Melanoma
Authors: Lazaridou Maria-Filothei , Massa Chiara , Handke Diana , Mueller Anja , Friedrich Michael , Subbarayan Karthikeyan , Tretbar Sandy , Dummer Reinhard , Koelblinger Peter , Seliger Barbara
Published: August 2020

The underlying molecular mechanisms of the aberrant expression of components of the HLA class I antigen processing and presentation machinery (APM) in tumors leading to evasion from T cell-mediated immune surveillance could be due to posttranscriptional regulation mediated by microRNAs (miRs). So far, some miRs controlling the expression of different APM components have been identified. Using in silico analysis and an miR enrichment protocol in combination with small RNA sequencing, miR-26b-5p and miR-21-3p were postulated to target the 3′ untranslated region (UTR) of the peptide transporter TAP1, which was confirmed by high free binding energy and dual luciferase reporter assays. Overexpression of miR-26b-5p and miR-21-3p in melanoma cells downregulated the TAP1 protein and reduced expression of HLA class I cell surface antigens, which could be reverted by miR inhibitors. Moreover, miR-26b-5p overexpression induced a decreased T cell recognition. Furthermore, an inverse expression of miR-26b-5p and miR-21-3p with TAP1 was found in primary melanoma lesions, which was linked with the frequency of CD8+ T cell infiltration. Thus, miR-26-5p and miR-21-3p are involved in the HLA class I-mediated immune escape and might be used as biomarkers or therapeutic targets for HLA class Ilow melanoma cells.
Salivary Cytokines in Children with Nephrotic Syndrome versus Healthy Children: A Comparative Study
Authors: Polak David , Borovitz Yael , Clyman-Levy Dana , Klein Yehuda , Bernfeld Nathalie , Davidovits Miriam , Davidovich Esti
Published: August 2020

Background: The aims of this study were to compare salivary cytokines and total protein between children with nephrotic syndrome (NS) and healthy children, and to examine whether saliva parameters can differentiate between steroid sensitivity and resistance and between disease remission and relapse. Methods: Twenty-seven children with nephrotic syndrome were classified according to steroid sensitivity and resistance, and disease remission and relapse. Twenty healthy children served as controls. Whole saliva samples were collected from all the participants. Urine and blood tests done on the same day as the saliva collection were recorded. Salivary total protein was quantified using bicinchoninic acid and IFNγ, IL-4, IL-8, IL-6, and IL1β levels using ELISA. Results: The mean ages of the nephrotic syndrome and control groups were 11.3 ± 2.4 and 9 ± 4.2, respectively. Compared to the control group, for the nephrotic syndrome group, total salivary protein was significantly lower, as were the levels of all the cytokines examined except IFNγ. Statistically significant differences were not found in any of the salivary markers examined between the children with nephrotic syndrome who were treatment sensitive (n = 19) and resistant (n = 8). Protein and IL-8 salivary levels were lower in the active (n = 7) than in the remission (n = 20) group. Conclusions: Salivary parameters distinguished children with nephrotic syndrome in relapse from healthy children. This may be due to decreased salivary protein excretion, which reflects decreased plasma levels, consequent to proteinuria. Accordingly, salivary markers may be developed as a diagnostic or screening tool for NS activity.
Conjunctival Ultraviolet Autofluorescence as a Measure of Riboflavin and Ultraviolet and Accelerated Cross-Linking Exposure in Keratoconic Patients
Authors: Waszczykowska Arleta , Bartosiewicz Krzysztof , Podgórski Michał , Zmysłowska-Polakowska Ewa , Jurowski Piotr
Published: August 2020

Purpose: The study was performed to analyze the prevalence of the conjunctival ultraviolet autofluorescence (CUVAF) area in keratoconic eyes and changes caused by UVA-irradiation as a component of accelerated corneal cross-linking (aCXL). Methods: The study group involved 20 keratoconic patients subjected to aCXL surgery in one eye. The comparative group consisted of 111 age- and sex-matched patients with healthy corneas. The images of the anterior segment in both patient groups were taken using a Coroneo camera. In the study group the photos were taken before and immediately after the surgery, and 7 and 30 days following the procedure. Results: Nasal and temporal autofluorescence area (AN+T) were significantly smaller in a keratoconic patients group compared to control group (p = 0.0001). Patients with the third stage of keratoconus had significantly higher AN+T (p = 0.0277) compared with individuals with lower stage keratoconus. No statistically significant CUVAF changes were observed after the aCXL procedure. In keratoconic patients with primary CUVAF undergoing aCXL, a temporary fast enlargement of the autofluorescence area was observed. Conclusions: The eyes undergoing the aCXL procedure showed no difference in the size of the CUVAF area but such patients should be in strict follow-up in order to reveal UV-related ocular surface diseases.
CFTR Modulators: Impact on Fertility, Pregnancy, and Lactation in Women with Cystic Fibrosis
Authors: Taylor-Cousar Jennifer L.
Published: August 2020

Cystic fibrosis (CF) is a life-shortening genetic disorder caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. These mutations lead to abnormal ion transport in mucous membranes throughout the body, including in the respiratory and gastrointestinal and reproductive tracts. Improvements in care and therapy have led to substantial increases in the quantity and quality of life for those with CF. Consequently, women with CF are increasingly interested in having families. Although pregnancy was once discouraged for women with CF, at this point, even women with moderately severe lung disease can successfully navigate pregnancy. With the recent approval of a triple combination CFTR modulator therapy that improves lung function, nutritional status, and quality of life for people with a single copy of the most common CFTR mutation, it is expected that the number of women with CF who choose to become pregnant will continue to increase. Although animal reproduction models show no alarming signals for use during pregnancy at normal human doses, there is a paucity of human safety data in pregnancy and lactation. This review summarizes what is currently known about the impact of use of CFTR modulators on fertility, pregnancy, and lactation in women with CF.
Dynamic Risk Stratification for Predicting Treatment Response in Differentiated Thyroid Cancer
Authors: Giannoula Evanthia , Melidis Christos , Papadopoulos Nikitas , Bamidis Panagiotis , Raftopoulos Vasilios , Iakovou Ioannis
Published: August 2020

Prognosis in Differentiated Thyroid Cancer (DTC) patients is excellent, but a significant degree of overtreatment still exists because of the inability to accurately identify small patient cohorts who experience a more aggressive form of the disease, often associated with certain poor prognostic factors. Identifying these cohorts at an early stage would allow patients at high risk to receive more aggressive treatment while avoiding unnecessary and invasive treatments in those at low risk. Most risk stratification systems include age, tumor size, grade, presence of local invasion, and regional or distant metastases. Here we discuss these common factors as well as their association with treatment response, but also other upcoming markers including histology and multifocality of primary tumor, dose administered and preparation method for Radioiodine Therapy (RAI), Thyroglobulin (Tg), Anti-thyroglobulin Antibodies (Tg-Ab) levels both at initial management and during follow-up, and the presence of previously existing benign thyroid disease. In addition, we examine the role of remnant size and avidity as well as surgeons’ experience in performing thyroidectomies with recurrence rate, discussing its impact on disease prognosis. Our results reveal that treatment response has a statistically significant association with histology, T and M stages, surgeons’ experience, Tg levels and remnant score both during RAI and follow up and Tg-Ab levels during follow-up whole body scan (WBS).
The Relationship between Sea-Swell Bound Wave Height and Wave Shape
Authors: de Wit Floris , Tissier Marion , Reniers Ad
Published: August 2020

The nonlinear wave shape, expressed by skewness and asymmetry, can be calculated from surface elevation or pressure time series using bispectral analysis. Here, it is shown that the same analysis technique can be used to calculate the bound superharmonic wave height. Using measured near-bed pressures from three different field experiments, it is demonstrated that there is a clear relationship between this bound wave height and the nonlinear wave shape, independent of the measurement time and location. This implies that knowledge on the spatially varying bound wave height can be used to improve wave shape-induced sediment transport predictions. Given the frequency-directional sea-swell wave spectrum, the bound wave height can be predicted using second order wave theory. This paper shows that in relatively deep water, where conditions are not too nonlinear, this theory can accurately predict the bispectrally estimated bound superharmonic wave height. However, in relatively shallow water, the mismatch between observed and predicted bound wave height increases significantly due to wave breaking, strong currents, and increased wave nonlinearity. These processes are often included in phase-averaged wind-wave models that predict the evolution of the frequency-directional spectrum over variable bathymetry through source terms in a wave action balance, including the transfer of energy to bound super harmonics. The possibility to calculate and compare with the observed bound super harmonic wave height opens the door to improved model predictions of the bound wave height, nonlinear wave shape and associated sediment transport in large-scale morphodynamic models at low additional computational cost.
Holocene Boulder Beach Eroded from Chromite and Dunite Sea Cliffs at Støypet on Leka Island (Northern Norway)
Authors: Johnson Markes E.
Published: August 2020

This project examines the role of high-latitude storms degrading a Holocene coast formed by igneous rocks composed of low-grade chromite ore and dunite that originated within the Earth’s crust near the upper mantle. Such rocks are dense and rarely exposed at the surface by tectonic events in the reconfiguration of old ocean basins. An unconsolidated boulder beach occupies Støypet valley on Leka Island in northern Norway, formerly an open channel 10,000 years ago when glacial ice was in retreat and rebound of the land surface was about to commence. Sea cliffs exposing a stratiform ore body dissected by fractures was subject to wave erosion that shed large cobbles and small boulders into the channel. Competing mathematical equations are applied to estimate the height of storm waves impacting the channel floor and cliffs, and the results are compared with observations on wave heights generated by recent storms striking the Norwegian coast with the intensity of an orkan (Norwegian for hurricane). Lateral size variations in beach clasts suggest that Holocene storms struck Leka Island from the southwest with wave heights between 5 and 7.5 m based on the largest beach boulders. This result compares favorably with recent high-latitude storm tracks in the Norwegian Sea and their recorded wave heights. The density of low-grade chromite ore (3.32 g/cm3) sampled from the beach deposit exceeds that of rocks like limestone or other igneous rocks such as rhyolite, andesite, and basalt taken into consideration regarding coastal boulder deposits associated with classic hurricanes in more tropical settings.
Differential Regulation of Echinocandin Targets Fks1 and Fks2 in Candida glabrata by the Post-Transcriptional Regulator Ssd1
Authors: Healey Kelley R. , Paderu Padmaja , Hou Xin , Jimenez Ortigosa Cristina , Bagley Nicole , Patel Biren , Zhao Yanan , Perlin David S.
Published: August 2020

Invasive infections caused by the opportunistic pathogen Candida glabrata are treated with echinocandin antifungals that target β-1,3-glucan synthase, an enzyme critical for fungal cell wall biosynthesis. Echinocandin resistance develops upon mutation of genes (FKS1 or FKS2) that encode the glucan synthase catalytic subunits. We have analyzed cellular factors that influence echinocandin susceptibility and here describe effects of the post-transcriptional regulator Ssd1, which in S. cerevisiae, can bind cell wall related gene transcripts. The SSD1 homolog in C. glabrata was disrupted in isogenic wild type and equivalent FKS1 and FKS2 mutant strains that demonstrate echinocandin resistance (MICs ˃ 0.5 µg/mL). A reversal of resistance (8- to 128-fold decrease in MICs) was observed in FKS1 mutants, but not in FKS2 mutants, following SSD1 deletion. Additionally, this phenotype was complemented upon expression of SSD1 from plasmid (pSSD1). All SSD1 disruptants displayed susceptibility to the calcineurin inhibitor FK506, similar to fks1∆. Decreases in relative gene expression ratios of FKS1 to FKS2 (2.6- to 4.5-fold) and in protein ratios of Fks1 to Fks2 (2.7- and 8.4-fold) were observed in FKS mutants upon SSD1 disruption. Additionally, a complementary increase in protein ratio was observed in the pSSD1 expressing strain. Overall, we describe a cellular factor that influences Fks1-specific mediated resistance and demonstrates further differential regulation of FKS1 and FKS2 in C. glabrata.
Is Artificial Intelligence Ready to Assess an Enterprise’s Financial Security?
Authors: Melnychenko Oleksandr
Published: August 2020

This study contributes to the literature on financial security by highlighting the relevance of the perceptions and resulting professional judgment of stakeholders. Assessing a company’s financial security using only economic indicators—as suggested in the existing literature—would be inaccurate when undertaking a comprehensive study of financial security. Specifically, indices and indicators based on financial or managerial reporting calculated at any particular point in time, provide only a superficial understanding—and may even distort the overall picture. It has also been suggested that expert assessment is the most objective method, although it has disadvantages related to individual cognitive limitations. These limitations are not particular to artificial intelligence, which could assess an enterprise’s financial security in a less biased way. However, by only imitating human behavior, it is not able to perceive and evaluate with intuition the dynamics of the company’s development and holistically assess the financial condition—despite the possibility of learning and forecasting—because artificial intelligence is not able to think and predict, which, in an enterprise, is the most important skill of a manager. Therefore, the risk of developing artificial intelligence to assess a firm’s financial security lies in a biased assessment of the enterprise’s activities in general—and its financial security in particular.
Has China’s New Round of Collective Forestland Tenure Reform Caused an Increase in Rural Labor Transfer?
Authors: Yang Liu , Ren Yang
Published: August 2020

To investigate the rural labor transfer effects of China’s Collective Forestland Tenure Reform (CFTR), we employ binary probit models by using survey data of 694 households from China’s northern collective forest areas. The results reveal that the improved property rights, including rights for forestland use, disposition and beneficiaries, and refined tenure security under the CFTR generally have caused an increase in rural household labor transfer to the nonfarm sector. Besides, we find that forestry-dominated households’ risk perceptions on forestland reallocation and expropriation have significantly dampened rural labor transfer to the nonfarm sector. These can be explained by the fact that the strengthening of forestland property rights brought about by the CFTR can promote an increase in the forestland transfer rate and improve the forestland relocation efficiency. This, in turn, will lead to the liberation of the rural labor force, increase the non-agricultural employment rate of farmers, and ultimately lead to an increase in the rural labor transfer to the nonfarm sector. Therefore, these findings indicate that to motivate rural labor transfer to the nonfarm sector in the context of the Chinese government’s call for urbanization and other developing countries’ handling of similar circumstances, policymakers should further refine household forestland property rights and better protect forestland tenure security by continuing to improve related forestry policies.
The Policy of Ecological Forest Rangers (EFRs) for the Poor: Goal Positioning and Realistic Choices—Evidence from the Re-Employment Behavior of EFRs in Sichuan, China
Authors: Yan Zhongcheng , Wei Feng , Chen Yaru , Deng Xin , Qi Yanbin
Published: August 2020

Ecological or environmental compensation policies are usually designed with multiple policy objectives such as protecting the ecological environment and promoting farmers’ livelihoods, but in the enforcement process, there are often inconsistencies between realistic choices and policy objectives. Based on pooled cross-section data from the 2017–2019 public announcement of the selection of ecological forest rangers (EFRs, who mainly refers to manage and protect forests, grasslands, deserts and rivers, and report or prevent the situation or behavior of the forest area disasters, animal and plant resources, and infrastructure damage in time) among the poor in Sichuan Province in China, we used the Probit model to analyze the influencing factors of the re-employment behavior of EFRs among the poor, with the aim of assessing the differences between central government goal positioning and local government enforcement options. We find that (1) EFRs from poor households who have not yet escaped poverty and have a high per capita income level are given priority to be re-employed. This finding shows that the policy of ecological forest rangers for the poor (PEFRP, it mainly refers to an environmental protection policy that only hires the poor) pays close attention to poverty reduction goals, but it does not consider the poorest people because the EFRs with a higher income obtain higher re-employment opportunities. (2) Age, health, and education, which represent the human capital level, have no significant impact on renewal. This finding shows that the local government has not jointly achieved the goal of “poverty reduction and environmental protection” in the enforcement of the PEFRP and has deviated from the initial goal positioning of the central government. Therefore, in order to achieve the multiple policy objectives such as poverty reduction and environmental protection together, future policy enforcement needs to be adjusted in terms of local administrative assessment and the selection and recruitment of EFRs.
Mapping Environmental Conflicts Using Spatial Text Mining
Authors: Lee Jae-hyuck , Kim Do-kyun
Published: August 2020

Mapping the characteristics and extent of environmental conflicts related to land use is important for developing regionally specific policies. However, because it is only possible to verify the frequency of conflicts on a specific predetermined subject, it is difficult to determine the various reasons for conflicts in a region. Therefore, this study mapped the current status of regional environmental conflicts in South Korea using a spatial text mining technique, then proposed relevant management policies. The results were obtained by analyzing environmental conflict data extracted from the online agendas of regional environmental organizations. Air quality-related conflicts in South Korea are concentrated in western municipalities; development-related conflicts are concentrated in the southern region of Jeju Island; and intensive safety-related conflicts occur in metropolitan areas, particularly Ulsan. Thus, the type of conflict is determined by the local environment, in accordance with the definition of environmental conflict, and the distribution is determined by the location of the stakeholder population. This study reveals the issues and locations related to local environmental conflict that require further attention, and proposes more wide-ranging methods for managing the links between conflicts by mapping environmental conflicts on a large scale rather than on an individual basis.
Combination of Digital and Conventional Workflows in the CAD/CAM-Fabrication of an Implant-Supported Overdenture
Authors: Schmidt Michael Benno , Rauch Angelika , Schwarzer Marcus , Lethaus Bernd , Hahnel Sebastian
Published: August 2020

Completely digital workflows for the fabrication of implant-supported removable restorations are not yet common in clinical dental practice. The aim of the current case report is to illustrate a reliable and comfortable workflow that reasonably merges conventional and digital workflows for the CAD/CAM-fabrication of implant-supported overdentures. The 53-year old patient was supplied with a digitally processed complete denture in the upper jaw and, simultaneously, with an overdenture supported by four interforaminal implants in the lower jaw. The overdenture included a completely digitally processed and manufactured alloy framework that had been fabricated by selective laser sintering. The case report indicates that digital manufacturing processes for extensive and complex removable restorations are possible. However, as it is currently not yet possible to digitally obtain functional impressions, future developments and innovations might focus on that issue.
Molecular Dynamics Simulation on Thin-Film Lubrication of a Mixture of Three Alkanes
Authors: Du Run , Zhang Anying , Du Zhihua , Zhang Xiaoyu
Published: August 2020

We used the COMPASS forcefield to perform molecular dynamics (MD) simulation of a mixture composed of three alkanes as the lubricant for the thin-film lubrication. The viscosity of the lubrication film in the non-working state, the final film thickness, and density distribution were investigated. The results reveal that the viscosity error among different initial film thicknesses in the non-working state is within 5%, which confirms the applicability of the model and the forcefield. The viscosity decreases oscillating as temperature increases. Whatever the initial film thickness is, the film thickness change rate with respect to pressure load is almost the same. When pressure increases, the density peaks increase. As the initial film thickness increases, the normalized thicknesses of adsorption and ordered layers decrease. In nanoscale, the density predicted by the MD simulation is higher than the prediction of the Tait equation, even if the adsorption layers is excluded.
An Investigation on the Microstructure and Texture of an AZ80 Cup-Shaped Piece Processed by Rotating Backward Extrusion
Authors: Che Xin , Dong Beibei , Liu Kai , Wang Qiang , Meng Mu , Gao Zhi , Ma Jin , Yang Fulai , Zhang Zhimin
Published: August 2020

The rotating backward extrusion (RBE) process, as a new severe plastic deformation (SPD) process, is based on conventional backward extrusion and rotation, which meets the requirement of modern industrial development with its high performance and production efficiency. However, there is little research on the microstructure evolution and texture modification of the RBE process. Thus, in this study, the effect of different rotating revolutions, e.g., n = 5, n = 10, and n = 50, on the microstructure and texture development for the RBE process based on the AZ80 magnesium (Mg) alloy were investigated at 653 K. The results disclose that the rotating revolution is an influencing processing parameter on the deformation of the RBE process. The grain refining ability is enhanced with the increase of the rotating revolutions, and the minimum grain size of the cup bottom, shearing zone, and cup wall can reach to 16.7 μm, 15.6 μm, and 13.0 μm, respectively, under the condition of n = 50. Furthermore, with the increase in the rotating revolutions, the microstructure of the alloy becomes more uniform and the proportion of dynamic recrystallization (DRX) is also increased. The maximum DRX fractions of the sample for the cup bottom and cup wall are 95.4% and 86.8%, respectively, at n = 50. The DRX mechanism of the RBE process is determined by the continuous DRX and discontinuous DRX. In addition, the texture can be significant weakened during the RBE process, especially at the cup bottom, where the maximum pole intensity can be reduced from 17.6 at n = 10 to 6.5 at n = 50, which can be attributed to the higher proportion of new DRXed grains whose orientations are more random compared with the deformed grains.
Profile and Areal Surface Parameters for Fatigue Fracture Characterisation
Authors: Macek Wojciech , Branco Ricardo , Szala Mirosław , Marciniak Zbigniew , Ulewicz Robert , Sczygiol Norbert , Kardasz Piotr
Published: August 2020

Post-mortem characterisation is a pivotal tool to trace back to the origin of structural failures in modern engineering analyses. This work compared both the crack propagation and rupture roughness profiles based on areal parameters for total fracture area. Notched and smooth samples made of weather-resistant structural steel (10HNAP), popular S355J2 structural steel and aluminium alloy AW-2017A under bending, torsion and combined bending–torsion were investigated. After the fatigue tests, fatigue fractures were measured with an optical profilometer, and the relevant surface parameters were critically compared. The results showed a great impact of the loading scenario on both the local profiles and total fracture areas. Both approaches (local and total fracture zones) for specimens with different geometries were investigated. For all specimens, measured texture parameters decreased in the following order: total area, rupture area and propagation area.
Flexural Properties of Renewable Coir Fiber Reinforced Magnesium Phosphate Cement, Considering Fiber Length
Authors: Zhang Liwen , Jiang Zuqian , Wu Hui , Zhang Wenhua , Lai Yushan , Zheng Weile , Li Jing
Published: August 2020

Coir fiber (CF), a renewable natural plant fiber, is more competitive in improving poor toughness and crack resistance of magnesium phosphate cement (MPC) than artificial fibers, due to its slight energy consumptions and low costs in production and waste treatment. In this paper, a typical three-point bending test was carried out to study the effects of CF length on MPC flexural properties. A total of forty-two cuboid specimens were employed to investigate the flexural strength, load-deflection behavior, and flexural toughness of MPC, with CF lengths varying from 0 to 30 mm at the curing age of 7 days and 28 days. Results showed that, at both two curing ages, MPC flexural strength first increased with CF length increasing, and then deceased when CF length exceeded the threshold. However, with the increase of CF length, MPC flexural toughness increased continuously, while MPC elastic modulus displayed a decreasing trend. Additionally, Modern micro testing techniques, such as scanning electron microscope (SEM) and X-ray diffraction (XRD), were also used to study the microstructure and phase compositions of specimens for further explaining the themicroscopic mechanism.
Optimizing Silanization to Functionalize Stainless Steel Wire: Towards Breast Cancer Stem Cell Isolation
Authors: Bekmurzayeva Aliya , Dukenbayev Kanat , Azevedo Helena S. , Marsili Enrico , Tosi Daniele , Kanayeva Damira
Published: August 2020

Chemically modified metal surfaces have been used to recognize and capture specific cell types and biomolecules. In this work, stainless steel wires were functionalized with aptamers against breast cancer stem cell markers. Stainless steel wires were first electropolished and silanized via electrodeposition. Aptamers were then attached to the silanized surface through a cross-linker. The functionalized wires were able to capture the target cells in an in vitro test. During surface modification steps, wires were analyzed by atomic force microscopy, cyclic voltammetry, scanning electron and fluorescence microscopy to determine their surface composition and morphology. Optimized conditions of silanization (applied potential, solution pH, heat treatment temperature) for obtaining an aptamer-functionalized wire were determined in this work together with the use of several surface characterization techniques suitable for small-sized and circular wires. These modified wires have potential applications for the in vivo capture of target cells in blood flow, since their small size allows their insertion as standard guidewires in biomedical devices.
High MB Solution Degradation Efficiency of FeSiBZr Amorphous Ribbon with Surface Tunnels
Authors: Chen Qi , Yan Zhicheng , Zhang Hao , Zhang Lai-Chang , Ma Haijian , Wang Wenlong , Wang Weimin
Published: August 2020

The as spun amorphous (Fe78Si9B13)99.5Zr0.5 (Zr0.5) and (Fe78Si9B13)99Zr1 (Zr1) ribbons having a Fenton-like reaction are proved to bear a good degradation performance in organic dye wastewater treatment for the first time by evaluating their degradation efficiency in methylene blue (MB) solution. Compared to the widely studied (Fe78Si9B13)100Zr0 (Zr0) amorphous ribbon for degradation, with increasing cZr (Zr atomic content), the as-spun Zr0, Zr0.5 and Zr1 amorphous ribbons have gradually increased degradation rate of MB solution. According to δc (characteristic distance) of as-spun Zr0, Zr0.5 and Zr1 ribbons, the free volume in Zr1 ribbon is higher Zr0 and Zr0.5 ribbons. In the reaction process, the Zr1 ribbon surface formed the 3D nano-porous structure with specific surface area higher than the cotton floc structure formed by Zr0 ribbon and coarse porous structure formed by Zr0.5 ribbon. The Zr1 ribbon’s high free volume and high specific surface area make its degradation rate of MB solution higher than that of Zr0 and Zr0.5 ribbons. This work not only provides a new method to remedying the organic dyes wastewater with high efficiency and low-cost, but also improves an application prospect of Fe-based glassy alloys.
Effect of Hot Rolling on the Microstructure and Properties of Nanostructured 15Cr ODS Alloys with Al and Zr Addition
Authors: Li Zhengyuan , Chen Lijia , Zhang Haoyu , Zhang Siqian , Zhang Zhipeng , Liu Siyu
Published: August 2020

Oxide dispersion strengthened (ODS) alloys with Al and Zr addition have excellent radiation tolerance, high-temperature strength, and corrosion resistance. The 15Cr-Al-Zr-ODS alloys are processed by mechanical alloying (MA), hot isostatic pressing (HIP), subsequent hot rolling to large strains of 70%, and further annealing. The effect of hot rolling on the microstructure, and the properties of nanostructured 15Cr ODS alloys with Al and Zr addition, were investigated. The microstructure after hot rolling and annealing showed obvious anisotropy. The cubic texture (φ1 = 0°, Φ = 0°, φ2 = 0°) {0 0 1} <1 0 0> and brass-R texture (φ1 = 0°, Φ = 55°, φ2 = 45°) {1 1 1} <1 1 0> were observed. The similar size distribution of precipitates was obtained for the comparison of the hot rolling samples with the hot isostatic pressed samples, which can be attributed to excellent thermal stability. After hot rolling, the alloy showed higher yield strength but did not lose too much plasticity.
From Atomic Level to Large-Scale Monte Carlo Magnetic Simulations
Authors: Chrobak Artur , Ziółkowski Grzegorz , Chrobak Dariusz , Chełkowska Grażyna
Published: August 2020

This paper refers to Monte Carlo magnetic simulations for large-scale systems. We propose scaling rules to facilitate analysis of mesoscopic objects using a relatively small amount of system nodes. In our model, each node represents a volume defined by an enlargement factor. As a consequence of this approach, the parameters describing magnetic interactions on the atomic level should also be re-scaled, taking into account the detailed thermodynamic balance as well as energetic equivalence between the real and re-scaled systems. Accuracy and efficiency of the model have been depicted through analysis of the size effects of magnetic moment configuration for various characteristic objects. As shown, the proposed scaling rules, applied to the disorder-based cluster Monte Carlo algorithm, can be considered suitable tools for designing new magnetic materials and a way to include low-level or first principle calculations in finite element Monte Carlo magnetic simulations.
Wavelet Element Modelling for Inviscid Fluid–Solid Coupling Problem based on Partitioned Approach
Authors: Yang Zhi-Bo , Li Hao-Qi , Qiao Bai-Jie , Chen Xue-Feng
Published: August 2020

To provide a simple numerical formulation based on fixed grids, a wavelet element method for fluid–solid modelling is introduced in this work. Compared with the classical wavelet finite element method, the presented method can potentially handle more complex shapes. Considering the differences between the solid and fluid regions, a damping-like interface based on wavelet elements is designed, in order to ensure consistency between the two parts. The inner regions are constructed with the same wavelet function in space. In the time and spatial domains, a partitioned approach based on Jacobi iteration is combined with the pseudo-parallel calculation method. Numerical convergence analyses show that the method can serve as an alternative choice for fluid–solid coupling modelling.