bluuebunny/crossref_metadata_2025 huggingface dataset:
Saline water, nitrogen and phosphorus on water relations and physiological aspects of West Indian cherry
Authors: Sá Francisco Vanies da Silva , Gheyi Hans Raj , Lima Geovani Soares de , Paiva Emanoela Pereira de , Lacerda Claudivan Feitosa , Fernandes Pedro Dantas
Published: November 2018

Salinity is a common problem in arid and semi-arid regions, causing great damage to crop yields, and management strategies that reduce the effects of salt stress on plants are necessary. So, the aim of this study was to evaluate the interaction between salinity, nitrogen and phosphorus on water relations, gas exchange and chloroplastidic pigments of the West Indian cherry in the vegetative/reproductive phase transition. The cultivation was carried out in a greenhouse, in lysimeters filled with an Entisol of low phosphorus content. The experiment was arranged in a randomized complete block design, arranged in a factorial scheme with two factors, being five levels of electrical conductivity of irrigation water (ECw)(0.6, 1.4, 2.2, 3.0 and 3.8 dS m-1) and four P:N rates (100:100, 140:100, 100:140 and 140:140% of recommendation) with three replications and one plant per plot. The analysis of results indicated that the seedlings of West Indian cherry cultivar BRS 366-Jaburu, grafted on the Criolo rootstock from the EMBRAPA Agroindústria Tropical in Pacajus-CE were used. Irrigation with saline water of up to 3.8 dS m-1 does not affect the water status of the West Indian cherry. Increased salinity of irrigation water reduces gaseous exchange activity and increases the percentage of leaf cell damage in the West Indian cherry. The 40% increase over recommended level in nitrogen supply increases the synthesis of chlorophyll b and carotenoids in the West Indian cherry, when irrigated with saline water of up to 2.2 dS m-1.
Intervención del ser humano en el proceso de destete de cabritos lecheros: parámetros fisiológicos y comportamiento animal
Authors: MARTINEZ Gabriela Marcela , Pereyra WR , SUAREZ Víctor H. , Alfaro EJ , ALFARO RJ , YANEZ RP
Published: November 2018

La intervención de los seres humanos en los procesos de cría y recría en animales de producción pueden tener diferente impacto en virtud al tipo, momento y duración de la misma. El objetivo del trabajo fue evaluar si existen cambios en parámetros fisiológicos, sanguíneos y de comportamiento en cabritos Saanen en función al tipo de crianza, 10 criados con sus madres (CT) vs. 10 criados artificialmente (CA), en las etapas de lactante y recría. Semanalmente se midió: frecuencia cardiaca (FC), temperatura rectal (TR), frecuencia respiratoria (FR), predisposición a ser atrapado y actitud de patear. Previo se tomaron 10 ml de sangre para realización del leucograma. El diseño experimental fue el de mediciones repetidas en el tiempo. Los datos fueron analizados a través del programa InfoStat. Ni entre periodos ni entre tratamientos se detectaron diferencias significativas para FC como TR. En ambos periodos a los animales del CT resultó más difícil atraparlos (lactante: p<0,0001, recría: p<0,0020). En la etapa de lactantes la cantidad de animales que pateaban fue superior (p<0,05) en CT, mientras que en recría no existieron diferencias. Durante la etapa de lactantes los conteos de neutrófilos del grupo CT fueron mayores (p<0,0001) y los de linfocitos menores (p<0,0001). Durante recría se hallaron diferencias (p<0,0001) entre grupos y la relación neutrófilos/linfocitos fue superior (p<0,0001) en un 50% en los CT. En función a lo obtenido es posible concluir que si bien existe un grado de estrés cuando el animal no habituado al ser humano se enfrenta a éste en manejos que ameriten su captura, el contacto en etapas tempranas permite aumentar el nivel de docilidad a la vez facilita la ejecución de tareas rutinarias.
THE KINETICS OF CaO ASSISTED PATTUKKU CHARCOAL STEAM GASIFICATION
Authors: Syarif Takdir , Sulistyo H , Sediawan Wahyudi B , Budhijanto B
Published: November 2018

Abstract Coal is a solid fuel that can be converted into syngas through gasification process. To obtain optimum gasification process design and operation, in-depth understanding of the influential parameters is required. This study aims to investigate the effect of temperature on the gasification process and to obtain its kinetics parameters. The study was carried out in a tubular reactor equipped with a heater and a condenser. Steam was used as gasifying agent, while CaO was employed as a CO2 adsorbent. The charcoal from coal was subjected to gasification at temperatures of 600°C, 700°C, and 800°C. The ratio of charcoal and CaO was 1:1. The gasification process lasted for 60 minutes with gas sample was taken every 15 minutes for composition analysis. The results showed that a temperature increase of 100°C caused a proportional increase of conversion of about 75% higher. The value of activation energy (Ea) and exponential factor (ko) were 46.645kJ/mole and 328.3894/min, respectively. For mass transfer parameters, values of activation energy for surface diffusion (Es) and surface diffusivity factor (as) were 81.126 kJ/mole and 0.138/min, respectively. Keywords: gasification; mathematical model; Pattukku coal char; steam; Thin Reaction Zone Model
THE DEVELOPMENT OF TRANSESTERIFICATION PROCESS OF COTTON SEED OIL BY USING MICROWAVE
Authors: Suryanto Andi , Sabara, HW Zakir , Artiningsih Andi , Ismail Hardi
Published: November 2018

Biodiesel is a renewable, non-toxic, environmentally friendly fuel made from vegetable oils through a transesterification reaction with methanol. During this time the manufacture of biodiesel takes a long time, which can be overcome with microwave heating. The use of microwave can decrease the reaction time and the amount of catalyst. The purpose of this study was to study the utilization of microwave as a heater in the transesterification reaction of cotton seed oil with the addition of NaOH catalyst 0.25, 0.5, 0.75 and 1% (w/w) with 100 watts microwave power and a reaction time of 15 minutes. Conversion of biodiesel from cotton seed oil with the NaOH catalyst concentrations 0.5% (w/w), 5 minutes, molar ratio of 1: 12 with a microwave power of 400 watts was 99.11%. The results of the analysis of several parameters on biodiesel products show that they have met the specifications based on Indonesian National Standard (SNI-04-7182-2006). Keyword: biodiesel, transesterification, cotton seed oil, microwave.
Influence of Microwave Irradiation on Extraction of Chitosan from Shrimp Shell Waste
Authors: Apriyanti Dwi Titik , Susanto Heru , Rokhati Nur
Published: November 2018

Chitosan is natural polysaccharides which is nontoxic, biodegradable, and biocompatible and have many advantages in various kinds of fields including health, food, agriculture, and industry. Chitosan usually take long time to extract by conventional method for deacetylation process of chitin. Raw material for chitosan can be found in shrimp shell waste. Chitosan manufactures usually need high temperatures and chemicals in large quantities and it takes much time and consumes a lot of energy where will give bad effect to the environment. Recently microwave irradiation as nonconventional energy sources is widely used in chemical reactions. To reduce the impact of environmental pollution due to excessive use of chemical treatment, the objective of this work is processing chitosan under microwave irradiation. Expected production of chitosan with the same mass requires fewer chemicals than conventional heating. In particular, the study will examine the effect of making the chitosan and adding chemicals, reaction time and operating temperature and degree of deacetylation in chitosan with conventional heating methods that the results will be compared using a microwave. In this research will be developed to the design and fabrication of prototype scale extractor for manufacturing chitosan from shrimp shell waste after optimum results obtained from the research laboratory scale. From the research we can conclude that microwave will speed up reaction time. FTIR also showed functional group of chitosan formed from microwave irradiation have same results.  Keywords: chitosan, shrimp shells, microwave
Lactic Acid Production From Cocoa Pod Husk by Studying Further the Influence of Alkaloids on Fermentation Process using Lactobacillus Plantarum Bacteria
Authors: Irwanto Dodi , Wiratni W. , Rochmadi R. , Syamsiah Siti
Published: November 2018

Cocoa Pod Husk (CPH) is the biomass waste from cocoa industry, generated after the digestion process. It accounts for about 70-75% of the cocoa fresh fruit. CPH contains active compounds of alkaloids that are thought to be the inhibitors of the lactic acid fermentation process using microorganisms. This study aims to produce Lactic Acid from CPH by studying further the influence of alkaloids on fermentation process using Lactobacillus plantarum bacteria. Fermentation using Lactobacillus plantarum bacteria was carried out at 50°C and with agitation at 100 rpm in incubator shaker for 48 h. This process was conducted to determine the effect of the addition of alkaloids by analyzing through the comparison between the consumption of substrate (glucose), dry weight of the cell, and the production of lactic acid. Evaluation of the differences in the performance of microorganisms at various treatments was performed based on the parameters values of the kinetic models prepared for the case studied. The kinetic model fitting results showed that the presence of alkaloids alters the growth patterns of products from growth-associated products into mixed patterns because the products were formed during slow growth and stationary phases. The maximum growth rate (μm) and substrate inhibition constant (Ks) obtained on each variation of inhibitor addition were likely to remain constant at the values of 0.69 h-1 and 3.89 g/L respectively, as these parameters were unaffected by the addition of inhibitor
IMPROVEMENT OF BIOETHANOL PRODUCTION BY USING Saccharomyces cerevisiae [Meyen ex E.C. Hansen] IMMOBILIZED ON PRETREATED SUGARCANE BAGASSE
Authors: Anita Sita Heris , Mangunwardoyo Wibowo , Yopi Yopi
Published: November 2018

Pretreated of sugarcane bagasse was used as a carrier for immobilization of Saccharomyces cerevisiae. Pretreatments were carried out by steaming, pressurized steam, and combination both of procedure.  The objectives of this research was to investigate the effect of pretreatment on sugarcane bagasse to cells adsorption and bioethanol production.  Immobilization process was conducted in a ratio of 2.5 g carrier/50 mL cell suspension.  Whole cell biocatalyst as much as 1% (w/v) was used as inoculum for bioethanol fermentation.  The best pretreated sugarcane bagasse for carrier of immobilized cells was obtained using steam treatment for 30 minutes.  Those treatment improved the physical properties of carrier and increased the cell retention up to 10.05 mg/g.  The use of whole cell biocatalyst after steaming pretreatment also enhanced ethanol yield 1.5 times higher than control. Keywords: bioethanol; immobilization; pretreatment; steam treatment; sugarcane bagasse
DEALUMINATION AND CHARACTERIZATION OF ZSM - 5 AS CATALYST FOR GLYCEROL CONVERSION TO GLYCEROL MONOLAURATE
Authors: Dwi Anggoro Didi , Rikardo Putra Riko , Oktaviani Herawati , Af’idatul Kamilah Lutfi , Chamdani FatmaTsaniya
Published: November 2018

Glycerol Monolaurate (GML) is a naturally occurring fatty acid widely utilized in food, cosmetics, andhomeopathic supplements. Glycerol is a compound glycerides, a byproduct of biodiesel production from the transesterification process. Glycerol is converted to glycerol derivative product that has more value as Glycerol Monolaurate (GML).GML is a naturally occurring fatty acid widely utilized in food, cosmetics, andhomeopathic supplements.One of the catalyst that had beenused is ZSM-5. Dealumination is used to change the acidity of the zeolite. This study consists of several stages, there are dealumination of zeolite ZSM-5 using H2SO4, drying at 110 ° C for 1 hour, Then calcination at a temperature of 550 ° C for 4 hours. Characterization catalyst to testing the acidity of the catalyst by absorption of ammonia and pyridine. Acidity of Zeolite is expressed in the mmol of ammonia or pyridine per gram of catalyst. Synthesis of monolaurate with dealuminated result are performed by GC-MS analysis, it’s to determine the molecular weight and to show a purity of Glycerol Monolaurate. Testing results obtained in the optimum conditions at dealumination temperature and dealumination time respectively are 40-60 0C and 2-5 hours. The dealumination variables are acid concentration, temperature and time of dealumination affecting ZSM-5 acidity and % yield of GML. The acidity affecting the yield% of Glycerol monolaurate produced Keywords :Glycerol, Glycerol Monolaurate, dealumination, ZSM - 5.
The effects of biofilm and selective mixed culture on the electricity outputs and wastewater quality of tempe liquid waste based microbial fuel cell
Authors: Surya Utami Tania , Arbianti Rita , Mariana M , Dwi Karina Nathania , Leondo Vifki
Published: November 2018

Microbial Fuel Cell (MFC) technology is highly prospective to be developed because it could be utilized as the alternative electricity sources and simultaneously as the wastewater treatment unit using microorganism as catalyst. Industrial Tempe wastewater has the potential to be used as MFC substrate since it still contains high nutrition for microbe and could pollute the environment if it disposed before being processed first. This study focused on investigating the effect of selective mixed culture addition and biofilm formation on the electricity production and the wastewater treatment aspects with tubular single chamber membranless reactor and industrial Tempe wastewater substrate. The result showed that, with the addition of selective mixed culture, the optimum electricity production obtained with addition of 1 ml gram-negative bacteria with increase in electricity production up to 92.14% and average voltage of 17.91 mV, while the optimum decreased levels of COD and BOD obtained with addition of 5 ml gram-negative bacteria which are 29.32% and 51.32%. On the biofilm formation experiment, optimum electricity production obtained from biofilm formation time for 14 days with increase in electricity production up to 10-folds and average voltage of 30.52 mV, while the optimum decreased levels of COD and BOD obtained from biofilm formation time for 7 days which are 18.2% and 35.9%.Keywords : biofilm, Microbial Fuel Cell, selective mixed culture, Tempe wastewater, tubular reactor
Transforming instruction to activity: Roleplay in language assessment
Authors: Kasper Gabriele , Youn Soo Jung
Published: November 2018

Roleplay is used as a method for education and training, assessment, and research across a wide range of academic and occupational domains, including applied linguistics. In the assessment of speaking and pragmatic competence, roleplay is used to examine how test takers produce and understand social action-in-interaction and in this way overcomes the problem of “construct under-representation”. Roleplay is also chosen for assessment purposes because it accommodates the opposing needs for authenticity and standardization in the design of assessment instruments. While the research literature is mainly concerned with the issue of how roleplay corresponds to real-life interaction, this study asks the more fundamental question of how participants manage to produce roleplays as intelligible unfolding social scenes in the first place. Specifically it explores how the roleplay setup becomes interactionally consequential in roleplays designed to assess the interactional competence of students in an English for academic purposes program. From the joint perspectives of conversation analysis and membership categorization analysis, the study demonstrates how the roleplay participants mobilize their generic and setting-specific interactional competences to accomplish the scenario as a shared practical activity. It shows how the participants jointly “talk the institution into being”, and what details from the setup they treat as necessary, optional, or dispensable. In this way the study reveals the local endogenous order of roleplay as a device for knowledge generation, training, and assessment and spawns further topics for research on roleplay design and interactional competence in a language assessment context.
Heterogeneic Time: An Anachronistic and Transcultural Rethinking of Eurochronology
Authors: Simonsen Karen-Margrethe
Published: November 2018

The focus of this article is the relation between time and transcultural space in literary history. The argument is that the concept of anachronism can help us understand the complexity of temporality and that the analysis of transcultural exchanges between European and Latin American art and literature may change literary history and further an understanding of the anachronism of Eurochronology. Two examples of a transcultural and anachronistic relation between Europe and America will be analyzed, both of them novels and writers of magical realism. Gabriel García Márquez’s novel Cien años de soledad (One Hundred Years of Solitude) is analyzed as a challenge to European historicity, while the transcultural aesthetics of Miguel Ángel Asturias is analyzed as a challenge to the understanding of avant-gardistic modernity. The analyses are carried out in critical dialogue with historicism and theories about world literature.
“Reopening the Grave”: Reading Trauma and Abjection in Hibakusha Poetry
Authors: Miller Alyson
Published: November 2018

Representations of the devastation of nuclear annihilation are undoubtedly confrontational, yet crucial to understanding the ongoing trauma and impact of atomic warfare. In examining how survivors “translate into words an extraordinarily painful landscape” (Tōge 1952), this paper explores the abject imagery utilized by hibakusha poets in order to express the violent horrors of the A-bomb. It focuses on how explicitly grotesque images function to give shape to events regarded as ineffable, and to make potently real the experiences of those whose identities were defined by shame and revulsion. Drawing upon Kristevan notions of abjection, and the poetry of hibakusha such as Kurihara Sadako, Tōge Sankichi, Kawamura Sachiko, and Shōda Shinoe, it contends that by seeking to graphically confront that which is ineffable, hibakusha poets are able to contest the liminal spaces to which their bodies and experiences have been relegated; indeed, by “reopening the grave” (Gotō, qtd. in Treat 1995, 29), survivor poets refuse silence, and give form and shape to trauma.
A Challenge to Global Literary History: The Case of World War I
Authors: Higonnet Margaret R.
Published: November 2018

This essay begins by investigating the possibility of a global literary history through the lens of periodization and its challenges for comparatists, starting from World War I. Second, by examining neglected texts from the periphery, it seeks to ‘provincialize’ the Eurocentric focus of our histories of war literature. To address the complex temporality of this epoch, we must accommodate the multicultural contexts from which these works emerge, as well as the long-term recovery of texts. Belatedness reflects the reemergence of memories from trauma, the discovery of manuscripts, the paucity of translations, and the long silencing of marginalized voices from the periphery. In turn, shifts in critical values and the translation of materials permit us to enlarge and reconstitute a globalized archive, as a few examples demonstrate. Great War texts by Huidobro, Svarnakumari, and Diallo as well as oral laments offer fruitful perspectives from the periphery on that epochal experience.
A note on bivariate Archimax copulas
Authors: Durante Fabrizio , Sánchez Juan Fernández , Sempi Carlo
Published: November 2018

We present an analytical proof of the characterisation of bivariate Archimax copulas in terms of the properties of their generating functions.
Stimulation of melanin synthesis in melanoma cells by cold plasma
Authors: Hasse Sybille , Müller Marie-Christine , Schallreuter Karin Uta , von Woedtke Thomas
Published: November 2018

Skin color is derived from epidermal melanocytes that contain specialized organelles in which melanin is formed. The formation of melanin is a well-orchestrated process, and reactive oxygen species (ROS) play a role in numerous enzymatic conversions, such as the reactions catalyzed by tyrosinase and tyrosine hydroxylase. Currently, there is ample evidence that cold plasma exerts biological effects on cells through the impact of ROS and reactive nitrogen species (RNS). Modulation of melanin biosynthesis by cold plasma has not yet been investigated. This study investigated melanin biosynthesis of human melanoma cell lines with different endogenous melanin contents (SK-Mel 28, G-361, FM-55-P and MNT-1) in response to cold plasma-derived reactive species. Initially, the distribution of melanosomes, via immunofluorescence, and the influence of microphthalmia-associated transcription factor (MiTF), as a key transcription factor, was investigated. In our experimental setup, all of the tested cell lines had an elevated melanin content after exposure to cold plasma. These promising results suggest a novel potential application of cold plasma for the regulation of melanogenesis and may be a useful tool for influencing skin color in the future.
Overseers of Upper Egypt
Authors: Brovarski Edward
Published: November 2018

Since my articles on the Overseers of Upper Egypt appeared in ZÄS 140 and ZÄS 141, I have become aware of three additional overseers. They are listed below.
Features of the hemicellulose structure of some species of regional raw materials and products of their enzymatic hydrolysis
Authors: Nikitina O. , Cherno N. , Ozolina S.
Published: November 2018

Nowadays, it is recognized that a lot of polysaccharides are biologically active. It is well known that these biomolecules show the highest level of their activity if they are water-soluble preparations, their molecular weight being 15–25 kDa, and if they preserve the supramolecular structure of carbohydrates. Basing on the fact that β-glucans of mushrooms are characterized by the antitumor, anticoagulant, anti-inflammatory, and immunomodulatory activities, it is important to determine whether regional raw material contains polysaccharides of a similar structure, and to define the conditions for their fragmentation to obtain products with a given molecular weight. The purpose of the work was to characterize the features of the structure of the hemicellulose complex of the Agaricus bisporous and Pleurotus ostreatus and products of their limited enzymatic hydrolysis. To determine the primary structure of hemicellulose polysaccharides, the 1H-NMR spectra of the samples were registered. It has been shown that β-D-(1→3)/β-(1→6)-glucan dominates in the hemicellulose of Pleurotus ostreatus. Among the hemicelluloses in the Agaricus bisporis, the main polysaccharide was galactoglucan. Its main chain consisted of β-D-glucopyranose residues interconnected with (1→3)-glucosidic bonds. The positions of O-6 monosaccharide are joined by the side branches in the form of β-D-glucopyranoses and the terminal residues of β-D-galactopyranoses. The hemicelluloses of Pleurotus ostreatus also contain manogalactan. Complexes of hemicelluloses of both types of mushrooms contain linear α-(1→3)-glucan in small quantities. It has been studied how the molecular-weight distribution of products of limited hydrolysis of hemicelluloses depends on the conditions of their treatment with the enzyme with β-(1→3)-glucanase activity. The maximum accumulation of a fraction with a given molecular weight of 15–25 kDa was observed at a ratio of E:S = 1:45 and treatment time of 21 hours. A specific reaction with congo red has proved there is a triple helical conformation of the main chain of the polysaccharide for this fraction of carbohydrates, so the supramolecular structure of the molecule is preserved.
Improving Procedural Skills, Teamwork and Confidence in just One Day: A NICU Fellow Boot Camp
Authors: Williams Sadie , Johnson Beth Ann , Geis Gary
Published: November 2018

This article was migrated. The article was marked as recommended. Objective: To develop a compact, simulation-based orientation session aimed at improving confidence and teamwork amongst new trainees.  Methods: Two cohorts of five fellows participated in the one-day boot camp. Confidence in team leading and neonatal procedures was assessed using a pre and post survey administered on the day of boot camp as well as a delayed post-test after 6 months. Teamwork and communication were assessed using the TEAM scale. Results: We found that fellows confidence was significantly improved in 5 out of 6 categories (team leader in code (p Conclusions: We demonstrated a significant improvement in confidence in first year neonatal-perinatal medicine fellows following a one-day simulation-based boot camp. The format for this boot camp could be emulated in institutions across the country to improve the confidence and skills of incoming fellows.
SIGNIFICANCE OF INFLAMMATION MARKERS DETERMINED IN PREGNANT WOMEN WITH ANEMIC SYNDROME AND OBESITY
Authors: Prokhorenko Т. S. , Zima A. P. , Saprina T. V. , Musina N. N. , Shakhmanova N. S. , Vasilyeva O. A. , Novitsky V. V.
Published: November 2018

The purpose of the present study was to specify a role of inflammatory mediators in pathogenesis of various types of anemia in pregnant obese women. We determined IL-1, IL-6, TNFα, C-reactive protein and hepcidin concentrations in blood serum of pregnant women with obesity depending on the type of anemic syndrome, either iron-deficiency anemia, or anemia of chronic diseases. We showed that the content of IL-6 in blood of the obese women exceeds the value of this index in healthy pregnant women (p < 0.05), and it does not depend on the presence and type of anemic syndrome. We found that the C-reactive protein concentration in pregnant women with obesity is higher than reference values (p < 0.05). Moreover, the contents of C-reactive protein in blood serum of pregnant women with anemia of chronic diseases is significantly higher (p < 0.05) than in women with iron deficiency anemia. Hepcidin concentration in blood of pregnant women with obesity and anemia of chronic disease was a specific feature: its content was significantly higher than in healthy pregnant women (p < 0.05), or in pregnant women with anemia-free obesity (p < 0.05). Hepcidin levels also exceeded 2-fold its contents in serum from pregnant women with obesity and iron deficiency anemia (p < 0.05). We have found that only pregnant women with obesity and anemia of chronic diseases have shown a positive correlation between the concentrations of C-reactive protein and blood levels of hepcidin (r = 0.733, p < 0.05), or IL-6 (r = 0.679, p < 0.05).The discussion concerns potential mechanisms of evolving anemia of chronic disease combined with subclinical inflammation in pregnant women with metabolic disorders. We conclude that a combination of obesity with gestational diabetes is a risk factor of anemia of chronic diseases in pregnant women. Development of an algorithm for differential diagnosis of iron deficiency anemia and anemia of chronic diseases in this cohort of patients is advisable for future studies in the area.
RANGING OF ANTIPHOSPOLIPID ANTIBODIES IN THE PATIENTS WITH THROMBOPHILIA AND RECURRENT MISCARRIAGE
Authors: Tkachenko O. Yu. , Lapin S. V , Shmonin A. A. , Solovyova L. N. , Bondareva E. A. , Selkov S. A. , Chepanov S. V. , Totolian Areg A. , Roggenbuck Dirk
Published: November 2018

Laboratory diagnosis of antiphospholipid syndrome (APS) is based on detection of antiphospholipid antibodies (aPLs). E.g., aPLs are directed against conformational epitopes of the so-called “co-factor” proteins: β2-gycoprotein 1 (β2-GP1), annexin V (An V) and prothrombin (Pt) that are formed during interaction with phospholipids – cardiolipin (CL), phosphatic acid (Pha), phosphatidylcholine (Pch), phosphatidylethanolamine (Pe), phosphatidylglycerol (Pg), phosphatidylinositol (Pi), phosphatidylserine (Ps). A routine methodology of detection based on ELISA testing is challenged by new tests when the antigen is absorbed on another kind of support like microbeads or membranes that can influence density of conformational epitopes for aPL’s binding. The aim of our study was to compare the results of aPLs detection by ELISA and multi-line immunodot assay (MLD). We collected blood serum samples from 45 patients with noncardioembolic ischemic strokes, 19 patients with recurrent deep vein thrombosis of lower limbs, 44 females with recurrent miscarriages, and 50 clinically healthy donors. To compare the results of aPL detection by ELISA and MLD kits, the test systems from different manufacturers were evaluated. We used an ELISA kits for detection of antibodies to CL IgG, aCL IgM, β2-GP1 produced by Euroimmun AG (Mr1) and Orgentec Diagnostica GmbH (Mr2) and MLD – for detection of antibodies to CL, β2-GP1, Pch, Pe, Pg, Pi, Ps, AnV and Pt (Medipan GmbH, Mr3). When a cut-off titer was used as the main index, 30.5% of patients were aPLs-positive with ELISA method by Mr1 and 38%, wiht Mr2. By MLD aPls were detected in 30% of patients. In the same cohort, medium and high aPLs titers (> 40 U/mL) were determined in 12% of patients using ELISA kits. Positive and highly positive aPLs titers were determined in 16% when using a new method by Mr3. Medium and high titer were detected only for antibodies to β2-GP1, CL, An V, Pha and Phs. The use of ELISA approach for detection of aPLs in patients with thrombosis and obstetric pathology is associated with relatively high number of low-positive ELISA results. Due to higher sensitivity for medium and high aPLs titers, MLD testing may be used as a confirming method for APS diagnosis.
Characterization and activity of endophytic bacteria from ‘Prata Anã’ banana crop (Musa sp., AAB)
Authors: Pereira Débora Francine Gomes Silva , Nietsche Silvia , Xavier Adelica Aparecida , Souza Suzane Ariádina de , Costa Márcia Regina , Duarte Anunciene Barbosa
Published: November 2018

ABSTRACT The objective of this study was to characterize banana tree endophytic bacteria at genus and species level and to determine the metabolic reactions associated with the nitrogen transformations. The identification at genus and species levels was performed using the partial sequencing of the rDNA 16S region. The assimbyotic nitrogen fixation, the reduction of nitrate and the production of urease were in vitro evaluated. The DNA of the bacterial isolates was also amplified to verify the presence of the nifH, nirK and nirS regions. Biochemical tests were performed in a complete randomized design; the treatments consisted of 39 bacterial isolates with three replications. Sequence analysis enabled the identification of four genera: Bacillus, Rhizobium, Klebsiella and Enterobacter. The Bacillus genus occurred more frequently, nine species were identified. By evaluating the results of biochemical tests, it was observed that three isolates showed multiple abilities: growth in NFb medium, nitrate reduction and production of urease. The isolates belong to the genus Bacillus and of the species subtilis, thuringienses and amyloliquefaciens. Approximately 12.5% of the isolates amplified the region corresponding to the nifH gene, 7.5% amplified gene nirK and 3.9% amplified the nirS gene. Endophytic bacteria evaluated in the present study showed in vitro activity for urease, nitrate reductase enzymes, however, relevant nitrogenase activity was not observed.
Estímulos químico e mecânico na rustificação de mudas de eucalipto
Authors: Lima Paulo Ricardo , Malavasi Ubirajara Contro , Dranski João Alexandre Lopes , Malavasi Marlene de Matos , Borsoi Augustinho , Ecco Martios
Published: November 2018

RESUMO Para o sucesso do plantio a campo e o êxito de um povoamento florestal, faz-se necessário o uso de mudas florestais que apresentem bom desempenho após o plantio. Este estudo objetivou avaliar a qualidade de mudas de eucalipto, após rustificação com aplicação de ácido jasmônico (JA) e flexões caulinares. O experimento foi realizado em duas etapas, sendo a primeira desenvolvida em ambiente protegido e a segunda realizada a campo. O delineamento experimental foi inteiramente ao acaso, com sete tratamentos (testemunha; 2,0; 4,0; 6,0 e 8,0 μmol L-1 de JA, aplicados semanalmente, por quatro semanas; 20 e 40 flexões caulinares diárias, por quatro semanas), com cinco repetições. Após as quatro semanas de aplicação dos tratamentos, foram determinados os incrementos de altura, de diâmetro do coleto e de número de folhas, de massas de matérias secas das raízes e da parte aérea, de potencial de regeneração de raízes e de índice SPAD. Aos 90 dias após o plantio, foram avaliados os incrementos de altura e de diâmetro do coleto e a relação altura/diâmetro das mudas. A análise de agrupamento por UPGMA evidenciou a formação de três grupos: grupo 1 (testemunha, 2,0; 4,0 e 6,0 μmol L-1 de JA); grupo 2 (8,0 μmol L-1 de JA) e grupo 3 (flexões caulinares). Mudas que apresentaram maior incremento de altura e de número de folhas (grupos I e II), durante a fase de rustificação, apresentaram maiores incrementos de altura e de diâmetro do coleto a campo, aos 90 dias após o plantio. Parâmetros como incrementos de diâmetro do coleto, de potencial de regeneração de raízes e de SPAD, quantificados durante a rustificação de mudas no viveiro, não são preditivos de maior desempenho a campo.
Os Relatórios do Desenvolvimento Humano publicados entre 2000 e 2005 e a construção de uma agenda pública sobre as despesas militares
Authors: Rezende Maria José de
Published: November 2018

Resumo Os Relatórios do Desenvolvimento Humano (RDHs) são documentos que sistematizam um grande número de diagnósticos, prescrições e propostas direcionadas, principalmente, aos governantes, Estados e lideranças políticas de modo geral (incluindo-se aquelas da sociedade civil organizada). O objeto desta pesquisa são as prescrições, contidas nos RDHs, de ações e procedimentos para expandir as fontes de financiamento de políticas que levem ao desenvolvimento humano. Entre as muitas sugestões estão aquelas direcionadas aos processos de contenção dos gastos militares e de ampliação das chances, nos países pobres, de formação de fundos para serem aplicados em políticas de desenvolvimento humano. Por se tratar de uma pesquisa documental, os RDHs são os materiais principais desta análise voltada para verificar, por meio de suas narrativas e argumentos, como seus formuladores constroem caminhos diversos para convencer diversos setores, grupos e segmentos sociais acerca da necessidade de encontrar fontes de financiamento das políticas que poderiam levar ao desenvolvimento humano.
O fim da divulgação dos resultados do Enem por escola: uma breve reflexão sobre a avaliação das políticas públicas e o acesso à informação
Authors: Zanchettin Fábio
Published: November 2018

Resumo Este estudo tem o objetivo de montar um cenário favorável à discussão sobre a decisão do Ministério da Educação (MEC) de deixar de divulgar os resultados do Exame Nacional do Ensino Médio (Enem) por escola. Em um primeiro momento, foi contextualizada e problematizada a questão sob aspectos jurídicos e de administração pública. A seguir, foram elaboradas estatísticas a partir da organização de dados obtidos nas planilhas “por escola” de anos anteriores, visando demonstrar a contribuição dessas informações para a avaliação das novas políticas públicas voltadas à educação. Na conclusão, retomaram-se os principais aspectos da discussão teórica apresentando os resultados mais relevantes da análise quantitativa de dados.
Reformar à esquerda: a administração pública gerencial em prefeituras petistas
Authors: Fontoura Leandro Heitich
Published: November 2018

Resumo Há duas décadas, o Partido dos Trabalhadores (PT) sustenta posição crítica à reforma do Estado realizada no governo Fernando Henrique Cardoso (PSDB). No discurso petista, a administração pública gerencial (APG) é apresentada como política neoliberal e conservadora e não iniciativa que pode ser politicamente orientada pela esquerda. Este artigo mostra uma revisão do discurso antirreforma. Diante dos problemas urbanos das cidades, dos entraves da burocracia pública e do aumento das demandas sociais, há prefeitos do partido implementando políticas associadas aos tucanos com a finalidade de modernizar a gestão e torná-la mais eficiente e efetiva. Esse cenário foi constatado em cinco municípios brasileiros, por meio de uma pesquisa qualitativa com prefeitos e secretários das áreas de Planejamento, Orçamento, Administração e Gestão.
Fragilidade na governança regional durante implementação da Rede de Urgência e Emergência em Região Metropolitana
Authors: Padilha Alexandre Rocha Santos , Amaral Marcia Aparecida , Oliveira Danilo Carvalho , Campos Gastão Wagner Souza
Published: November 2018

RESUMO Este artigo analisa a implementação da Rede de Urgência e Emergências, seus arranjos interfederativos regionais de pactuação e gestão de políticas, na Região Metropolitana de São Paulo, no período de 2011-2016. Considera-se implementação como um processo dinâmico da política, com interação, negociação e aprendizagem permanentes. Este é um estudo de caso, baseado na pesquisa qualitativa em saúde e nos estudos de avaliação de implementação de políticas, com 4 níveis de análise imbricados: desenho de implementação; caracterização de atores de implementação; caracterização de arranjos interfederativos regionais e contenciosos e desafios interfederativos não superados. Os dados foram obtidos em documentos públicos das instâncias de pactuação e coordenação da política e das organizações e dos atores participantes. Baseado nas reflexões de poder em políticas de saúde de Testa e no método Paideia de gestão de coletivos, analisa-se como os resultados revelam a insuficiência dos instrumentos políticos e dos arranjos de coordenação desenvolvidos pela implementação da Rede de Urgência e Emergência. Assim, discutem-se a fragilidade da governança regional, a necessidade de fortalecer as regiões de saúde como unidade territorial, sua relação com as Redes de Atenção à Saúde e as propostas de arranjos regionais e interfederativos para o desenvolvimento do Sistema Único de Saúde (SUS).
Apoio Matricial em saúde mental: a perspectiva dos especialistas sobre o processo de trabalho
Authors: Hirdes Alice
Published: November 2018

RESUMO O artigo aborda a descentralização em saúde mental por meio do Apoio Matricial na Atenção Primária à Saúde (APS). A pesquisa teve como objetivo geral investigar o Apoio Matricial em saúde mental na APS na perspectiva dos especialistas, levando em conta as diretrizes do Sistema Único de Saúde (SUS) e da Reforma Psiquiátrica. Trata-se de uma pesquisa qualitativa, realizada mediante entrevistas semiestruturadas e grupo focal com apoiadores matriciais em saúde mental. Os resultados evidenciam o aspecto processual da construção conjunta de intervenções sustentadas pela corresponsabilização e pela cogestão; os arranjos organizacionais e operacionais fundamentados em ações de supervisão, discussão de casos clínicos e atendimento conjunto. As relações personalizadas com as equipes de referência, o viés de saúde pública dos especialistas e o investimento em sensibilizar os profissionais generalistas e especialistas foram identificados na pesquisa. Conclui-se que o Apoio Matricial se sustenta por meio de tecnologias relacionais, na sistematicidade dos encontros e na longitudinalidade, devendo ser tais questões consideradas no cuidado interprofissional.
Impactos das práticas integrativas e complementares na saúde de pacientes crônicos
Authors: Dacal Maria del Pilar Ogando , Silva Irani Santos
Published: November 2018

RESUMO A inclusão das práticas integrativas e complementares nos serviços de saúde pública tem acontecido de forma gradual e lentificada, há cerca de 10 anos. Este estudo visa a colaborar com a ampliação do conhecimento sobre as práticas integrativas, tendo como objetivo principal apresentar os impactos do reiki e da reflexologia podal na saúde de pacientes crônicos atendidos em um centro especializado em endocrinopatias, localizado em Salvador (BA). Trata-se de um estudo observacional retrospectivo, de corte transversal, que utilizou registros em prontuários médicos. Observaram-se, a partir da análise dos dados, impactos aparentes das terapias complementares no alívio de sintomas psicológicos, emocionais e físicos, tais como ansiedade, estresse e dores no corpo.
TRADUÇÃO PARA LÍNGUA PORTUGUESA DO DATA SET TRATO URINÁRIO INFERIOR PARA INDIVÍDUOS COM LESÃO MEDULAR
Authors: Grillo Adriana Cordeiro da Silva , Faleiros Fabiana , Silva Josana Cristina Faleiros e , Tate Denise Galuf , Greve Júlia Maria D´Andrea , Tholl Adriana Dutra
Published: November 2018

RESUMO Objetivo: traduzir e adaptar para a língua portuguesa o data set do trato urinário inferior para indivíduos com lesão medular proposto pela International Spinal Cord Society e American Spinal Injury Association. Método: constitui-se de um estudo metodológico de tradução de instrumento, com a participação de um grupo de cinco especialistas que participaram das fases de tradução e retrotradução e cinco juízes. O Data set foi traduzido e adaptado segundo as recomendações metodológicas da International Spinal Cord Society e American Spinal Injury Association. Resultados: para a etapa de tradução, 56% das sentenças foram traduzidas com unanimidade pelos três tradutores convidados. Das nove questões que compunham o instrumento apenas quatro apresentaram divergências. Conclusão: obteve-se um instrumento traduzido e culturalmente adaptado para avaliação do trato urinário inferior de pessoas com lesão medular em língua portuguesa, proporcionando a participação brasileira nos estudos multicêntricos internacionais sobre lesão medular.
RISK FACTORS FOR EARLY HOSPITAL READMISSION FOLLOWING TOTAL KNEE ARTHROPLASTY
Authors: Lehtonen Eva J. , Hess Matthew C. , McGwin Jr. Gerald , Shah Ashish , Godoy-Santos Alexandre Leme , Naranje Sameer
Published: November 2018

ABSTRACT Objective: To identify independent risk factors, complications and early hospital readmission following total knee arthroplasty. Methods: Using the ACS-NSQIP database, we identified patients who underwent primary TKA from 2012-2015. The primary outcome was early hospital readmission. Patient demographics, preoperative comorbidities, laboratory data, operative characteristics, and postoperative complications were compared between readmitted and non-readmitted patients. Logistic regression identified independent risk factors for 30-day readmission. Results: 137,209 patients underwent TKA; 3.4% were readmitted within 30 days. Advanced age, male sex, black ethnicity, morbid obesity, presence of preoperative comorbidities, high ASA classification, and increased operative time were independently related risk factors. Asian and no reported race were negative risk factors. Postoperative complications: acute myocardial infarction, acute renal failure, stroke, pneumonia, pulmonary embolism, and deep vein thrombosis show positive associations. Conclusions: Advanced age, male sex, black ethnicity, morbid obesity, presence of comorbidities, high ASA classification and long operative time are independent risk factors for postoperative complications and early hospital readmission following total knee arthroplasty. Level of Evidence III, Case control study.
CASE STUDY OF CONGENITAL ANOMALIES OF THE UPPER LIMB IN REFERENCE AMBULATORY CARE FACILITY
Authors: Pinto Netto Henrique de Barros , Pais Antônio Pedro , Vitorio Simone Costa , Brandão Renata , Moreira Aline Aparecida Depianti , Molinaro Neto Luiz Raphael
Published: November 2018

ABSTRACT Objective: The epidemiological profile of congenital anomalies of the upper limbs (CAULs) is of major relevance to monitoring and planning. A study of this profile may reveal if there is prevalence of some specific type of malformation in comparison to a more comprehensive epidemiological sample. The Latin American Collaborative Study of Congenital Malformations (ECLAMC) has an extensive database, providing an excellent source of comparison. This study aims to evaluate the epidemiological profile of CAULs at the hand surgery department of the Hospital Federal da Lagoa (HFL) in Brazil, and compare it to the ECLAMC data. Methods: We conducted a retrospective analysis of patients who underwent treatment at the pediatric outpatient hand surgery clinic. The sample universe consisted of 126 patients (4 of these patients presented with 2 simultaneous anomalies), totaling 130 malformations. Results: The results demonstrated that the comparable pathologies have significantly similar incidence rates. It is worth noting the polydactylies (pre- and post-axial), where the percentile of incidence in the ECLAMC was higher. Conclusion: This study showed that the epidemiological profile of patients who underwent treatment at this hospital was equivalent to that found in the ECLAMC database. Level of evidence III, Retrospective epidemiological study.
ARTHROSCOPIC ASSESSMENT OF THE WRIST WITH KIENBÖCK’S DISEASE
Authors: Amariz Guilherme Augusto Silva , Abreu Maria Virginia Arranz , Veronesi Bruno Azevedo , Rezende Marcelo Rosa de
Published: November 2018

ABSTRACT Objective: Treatment of Kienböck's disease is challenging and the variability of results, despite the surgical technique, shows that there must be other aspects that can influence outcomes. The objective of this study is determine, through arthroscopic approach, the pattern of impairment of the carpal bones in Lichtman stage III patients. Method: Wrist arthroscopy was performed in patients with symptoms and evidence of lunate necrosis on x-rays, with signs of 3A and 3B stages. The Outerbridge classification was used to grade the articular status of the carpal bones. Results: Fifteen patients with stage 3 disease were assessed, five of whom were staged as type 3A and ten as 3B using the Lichtman stages. According to the Outerbridge classification, the lunate sulcus was the most affected with means of 2.8 at 3A and 2.9 at 3B. Other areas were significantly less affected with means of 1.1 and 1.3, respectively. Conclusion: Arthroscopic assessment of the wrist could aid in surgical decision making, offering more details on cartilage status in stage 3 Kienböck's disease. Level of Evidence III, Study of nonconsecutive patients, without consistently applied reference “gold” standard.
Campylobacter jejuni, Campylobacter coli, Salmonella spp. e Yersinia enterocolitica isoladas de animais silvestres em um centro de reabilitação
Authors: Silveira Débora R. , Milan Camile , Ferrasso Marina M. , Dias Priscila A. , Moraes Thamíris P. , Bandarra Paulo M. , Minello Luiz F. , Timm Cláudio D.
Published: November 2018

RESUMO: Muitas espécies de animais silvestres de vida livre servem como reservatório de bactérias patogênicas que ameaçam a saúde humana e dos animais domésticos. Algumas bactérias, como Campylobacter jejuni, Campylobacter coli, Yersinia enterocolitica e Salmonella enterica, causam enfermidades em humanos e podem contaminar os animais domésticos e silvestres. O Núcleo de Reabilitação da Fauna Silvestre da Universidade Federal de Pelotas (NURFS-UFPel) soluciona uma demanda regional específica de atenção à fauna silvestre brasileira. O objetivo desse trabalho foi identificar a presença de Campylobacter jejuni, Campylobacter coli, Salmonella spp. e Yersinia enterocolitica em animais silvestres que se encontravam em processo de reabilitação. Foram coletadas amostras de fezes, com uso de zaragatoas estéreis, de 34 aves, 16 mamíferos e 23 répteis. Dos 73 animais amostrados, quatro (5,48%) albergavam Y. enterocolitica, sendo duas aves, um mamífero e um réptil. Salmonella e Campylobacter não foram isolados. Os perfis de bandas dos isolados de Y. enterocolitica analisados pela rep-PCR foram diferentes entre si. Esses resultados indicam que as cepas isoladas não estão relacionadas entre si, não possuindo uma origem comum recente. Vanellus chilensis, Turdus rufiventris, Didelphis albiventris e Pantherophis guttatus podem albergar Y. enterocolitica e eliminá-la nas fezes, oferecendo risco de disseminação desse micro-organismo no ambiente, além de constituírem possíveis fontes de contaminação para humanos e outros animais.