bluuebunny/crossref_metadata_2025 huggingface dataset:
Prediction of EGFR Mutation Status in Non–Small Cell Lung Cancer Based on Ensemble Learning
Authors: Feng Youdan , Song Fan , Zhang Peng , Fan Guangda , Zhang Tianyi , Zhao Xiangyu , Ma Chenbin , Sun Yangyang , Song Xiao , Pu Huangsheng , Liu Fei , Zhang Guanglei
Published: June 2022

Objectives: We aimed to identify whether ensemble learning can improve the performance of the epidermal growth factor receptor (EGFR) mutation status predicting model.

Methods: We retrospectively collected 168 patients with non–small cell lung cancer (NSCLC), who underwent both computed tomography (CT) examination and EGFR test. Using the radiomics features extracted from the CT images, an ensemble model was established with four individual classifiers: logistic regression (LR), support vector machine (SVM), random forest (RF), and extreme gradient boosting (XGBoost). The synthetic minority oversampling technique (SMOTE) was also used to decrease the influence of data imbalance. The performances of the predicting model were evaluated using the area under the curve (AUC).

Results: Based on the 26 radiomics features after feature selection, the SVM performed best (AUCs of 0.8634 and 0.7885 on the training and test sets, respectively) among four individual classifiers. The ensemble model of RF, XGBoost, and LR achieved the best performance (AUCs of 0.8465 and 0.8654 on the training and test sets, respectively).

Conclusion: Ensemble learning can improve the model performance in predicting the EGFR mutation status of patients with NSCLC, showing potential value in clinical practice.
Recent Advances in Biofluid Mechanics and Bio- and Hemorheology Collating Recent Advances in Predicting Complex Behavior of Human Blood With Thixo-Elasto-Visco-Plastic Models and Sequence of Physical Process
Authors: Armstrong Matthew , Pincot Andre , Rogers Simon , Knight Thomas , Bailey Dorian
Published: June 2022

For years industrial polymer production has driven the development of rheological models to characterize the flow of materials. With the evolution of these models has come a corresponding advancement in the understanding of the complex mechanical properties. Recent efforts have been focused on modeling the behavior of complex fluids such as blood, whose microstructure leads to has simultaneous characteristics such as: thixotropy; elasticity; plasticity; and an evolving viscosity (part of which originates with the rouleaux’s evolution). The specific complex behavior of human blood can be analyzed via the analysis of Large-Amplitude-Oscillatory-Shear (LAOS) and Small-Amplitude-Oscillatory-Shear (SAOS) response tests. Unique features of human blood cannot be replicated in legacy steady-state models and, thus, have required the development of more comprehensive models capable of accurately fitting both steady state, transient flow and oscillatory shear flow. Expanding upon prior transient models, collaboration between the Chemical Engineering departments of the United States Military Academy and the University of Delaware has produced a new model, tensorial enhanced structural stress thixotropic-viscoelastic model (t-ESSTV). This model can capture the timescales contained within the plasma and individual red blood cells viscoelasticity and the thixotropic timescales associated with rouleaux breakdown and aggregation. The efficacy of t-ESSTV is demonstrated with a single Donor before consolidating the best fit model parameters of twelve Donor sets of rheological data. We then show the parametric correlations between model and physiological parameters and with the models’ prediction of microstructure, we correlate microstructure with the “elastic, solid-like” metrics as computed by Sequence of Physical Processes (SPP).
BAIBA Involves in Hypoxic Training Induced Browning of White Adipose Tissue in Obese Rats
Authors: Feng Junpeng , Wang Xuebing , Lu Yingli , Yu Chang , Wang Xinyan , Feng Lianshi
Published: June 2022

In recent years, obesity has become an important risk factor for human health; how to effectively prevent and reduce the occurrence of obesity is a hot research topic in recent years. Hypoxic training effectively improves abnormalities of lipid metabolism caused by obesity. The current study explored the effects of hypoxic training on BAIBA secretion and white fat browning in inguinal fat in obese rats. Analyses were performed by HPLC/MS/MS—MS/MS, RT-q PCR and western blot methods. The findings showed that 4 weeks of hypoxic training reduced body weight, Lee’s index, and regulated blood lipid profile in obese rats. Hypoxic training up-regulated BAIBA concentration in gastrocnemius muscle and circulation in obese rats. Hypoxic training significantly upregulated expression of PPARα and UCP-1 in inguinal fat of obese rats and increased white fat browning. The findings showed that BAIBA may involve in improveing blood lipid profile and white fat browning by modulating PPARα and UCP-1 expression.
Endoplasmic Reticulum Stress Contributes to Ventilator-Induced Diaphragm Atrophy and Weakness in Rats
Authors: Li Shaoping , Luo Guanguan , Zeng Rong , Lin Lian , Zou Xingnan , Yan Yu , Ma Haoli , Xia Jian , Zhao Yan , Zhou Xianlong
Published: June 2022

Background: Accumulating evidence indicates that endoplasmic reticulum (ER) stress plays a critical role in the regulation of skeletal muscle mass. In recent years, much attention has been given to ventilator-induced diaphragm dysfunction (VIDD) because it strongly impacts the outcomes of critically ill patients. Current evidence suggests that the enhancement of oxidative stress is essential for the development of VIDD, but there are no data on the effects of ER stress on this pathological process.

Methods: VIDD was induced by volume-controlled mechanical ventilation (MV) for 12 h; Spontaneous breathing (SB, for 12 h) rats were used as controls. The ER stress inhibitor 4-phenylbutyrate (4-PBA), the antioxidant N-acetylcysteine (NAC), and the ER stress inducer tunicamycin (TUN) were given before the onset of MV or SB. Diaphragm function, oxidative stress, and ER stress in the diaphragms were measured at the end of the experiments.

Results: ER stress was markedly increased in diaphragms relative to that in SB after 12 h of MV (all p < 0.001). Inhibition of ER stress by 4-PBA downregulated the expression levels of proteolysis-related genes in skeletal muscle, including Atrogin-1 and MuRF-1, reduced myofiber atrophy, and improved diaphragm force-generating capacity in rats subjected to MV (all p < 0.01). In addition, mitochondrial reactive oxygen species (ROS) production and protein level of 4-HNE (4-hydroxynonenal) were decreased upon 4-PBA treatment in rats during MV (all p < 0.01). Interestingly, the 4-PBA treatment also markedly increased the expression of peroxisome proliferator-activated receptor-gamma co-activator-1alpha (PGC-1α) (p < 0.01), a master regulator for mitochondrial function and a strong antioxidant. However, the antioxidant NAC failed to reduce ER stress in the diaphragm during MV (p > 0.05). Finally, ER stress inducer TUN largely compromised diaphragm dysfunction in the absence of oxidative stress (all p < 0.01).

Conclusion: ER stress is induced by MV and the inhibition of ER stress alleviates oxidative stress in the diaphragm during MV. In addition, ER stress is responsible for diaphragm dysfunction in the absence of oxidative stress. Therefore, the inhibition of ER stress may be another promising therapeutic approach for the treatment of VIDD.
Identification of Novel Variants of Thyroid Hormone Receptor Interaction Protein 13 That Cause Female Infertility Characterized by Zygotic Cleavage Failure
Authors: Hu Huiling , Zhang Shuoping , Guo Jing , Meng Fei , Chen Xueqin , Gong Fei , Lu Guangxiu , Zheng Wei , Lin Ge
Published: June 2022

Zygotic cleavage failure (ZCF) is a severe, early type of embryonic arrest in which zygotes cannot complete the first cleavage. Although mutations in BTG4 and CHEK1 have been identified as genetic causes of ZCF, these genes only explain a small population of ZCF cases. Thus, the underlying genetic causes for other affected individuals need to be identified. Here, we identified three TRIP13 missense variants responsible for ZCF in two patients and showed that they followed a recessive inheritance pattern. All three variants resulted in obvious changes in hydrogen bonding and consistent increase in DNA damage. Additionally, transcriptomic sequencing of oocytes and arrested embryos containing these variants suggested a greater number of differentially expressed transcripts in germinal vesicle (GV) oocytes than in 1-cell embryos. Vital genes for energy metabolism and cell cycle procession were widely and markedly downregulated, while DNA repair-related genes were significantly upregulated in both GV oocytes and 1-cell embryos of patients. These findings highlight a critical role of TRIP13 in meiosis and mitosis, as well as expand the genetic and phenotypic spectra of TR1P13 variants with respect to female infertility, especially in relation to ZCF.
From Western Asia to the Mediterranean Basin: Diversification of the Widespread Euphorbia nicaeensis Alliance (Euphorbiaceae)
Authors: Stojilkovič Valentina , Záveská Eliška , Frajman Božo
Published: June 2022

The Mediterranean Basin is an important biodiversity hotspot and one of the richest areas in the world in terms of plant diversity. Its flora parallels in several aspects that of the Eurasian steppes and the adjacent Irano-Turanian floristic region. The Euphorbia nicaeensis alliance spans this immense area from the western Mediterranean to Central Asia. Using an array of complementary methods, ranging from phylogenomic and phylogenetic data through relative genome size (RGS) estimation to morphometry, we explored relationships and biogeographic connections among taxa of this group. We identified the main evolutionary lineages, which mostly correspond to described taxa. However, despite the use of highly resolving Restriction Site Associated DNA (RAD) sequencing data, relationships among the main lineages remain ambiguous. This is likely due to hybridisation, lineage sorting triggered by rapid range expansion, and polyploidisation. The phylogenomic data identified cryptic diversity in the Mediterranean, which is also correlated with RGS and, partly, also, morphological divergence, rendering the description of a new species necessary. Biogeographic analyses suggest that Western Asia is the source area for the colonisation of the Mediterranean by this plant group and highlight the important contribution of the Irano-Turanian region to the high diversity in the Mediterranean Basin. The diversification of the E. nicaeensis alliance in the Mediterranean was triggered by vicariance in isolated Pleistocene refugia, morphological adaptation to divergent ecological conditions, and, to a lesser extent, by polyploidisation.
Whole-Genome DNA Methylation Analysis in Brassica rapa subsp. perviridis in Response to Albugo candida Infection
Authors: Tirnaz Soodeh , Miyaji Naomi , Takuno Shohei , Bayer Philipp E. , Shimizu Motoki , Akter Mst. Arjina , Edwards David , Batley Jacqueline , Fujimoto Ryo
Published: June 2022

DNA methylation is an epigenetic mark associated with several mechanisms in plants including immunity mechanisms. However, little is known about the regulatory role of DNA methylation in the resistance response of Brassica species against fungal diseases. White rust, caused by the fungus Albugo candida, is one of the most widespread and destructive diseases of all the cultivated Brassica species, particularly Brassica rapa L. and Brassica juncea (L.) Czern and Coss. Here, we investigate whole-genome DNA methylation modifications of B. rapa subsp. perviridis in response to white rust. As a result, 233 and 275 differentially methylated regions (DMRs) in the susceptible cultivar “Misugi” and the resistant cultivar “Nanane” were identified, respectively. In both cultivars, more than half of the DMRs were associated with genes (DMR-genes). Gene expression analysis showed that 13 of these genes were also differentially expressed between control and infected samples. Gene ontology enrichment analysis of DMR genes revealed their involvement in various biological processes including defense mechanisms. DMRs were unevenly distributed around genes in susceptible and resistant cultivars. In “Misugi,” DMRs tended to be located within genes, while in “Nanane,” DMRs tended to be located up and downstream of the genes. However, CG DMRs were predominantly located within genes in both cultivars. Transposable elements also showed association with all three sequence contexts of DMRs but predominantly with CHG and CHH DMRs in both cultivars. Our findings indicate the occurrence of DNA methylation modifications in B. rapa in response to white rust infection and suggest a potential regulatory role of DNA methylation modification in defense mechanisms which could be exploited to improve disease resistance.
Understanding the Nature of Hybrid Sterility and Divergence of Asian Cultivated Rice
Authors: Zhang Yu , Wang Jie , Pu Qiuhong , Yang Ying , Lv Yonggang , Zhou Jiawu , Li Jing , Deng Xianneng , Wang Min , Tao Dayun
Published: June 2022

Intraspecific hybrid sterility is a common form of postzygotic reproductive isolation in Asian cultivated rice, which is also the major obstacle to utilize the strong heterosis in the rice breeding program. Here, we review recent progress in classification and hybrid sterility in Asian cultivated rice. A genome-wide analysis of numerous wild relatives of rice and Asian cultivated rice has provided insights into the origin and differentiation of Asian cultivated rice, and divided Asian cultivated rice into five subgroups. More than 40 conserved and specific loci were identified to be responsible for the hybrid sterility between subgroup crosses by genetic mapping, which also contributed to the divergence of Asian cultivated rice. Most of the studies are focused on the sterile barriers between indica and japonica crosses, ignoring hybrid sterility among other subgroups, leading to neither a systematical understanding of the nature of hybrid sterility and subgroup divergence, nor effectively utilizing strong heterosis between the subgroups in Asian cultivated rice. Future studies will aim at identifying and characterizing genes for hybrid sterility and segregation distortion, comparing and understanding the molecular mechanism of hybrid sterility, and drawing a blueprint for intraspecific hybrid sterility loci derived from cross combinations among the five subgroups. These studies would provide scientific and accurate guidelines to overcome the intraspecific hybrid sterility according to the parent subgroup type identification, allowing the utilization of heterosis among subgroups, also helping us unlock the mysterious relationship between hybrid sterility and Asian cultivated rice divergence.
Relevance and Regulation of Alternative Splicing in Plant Heat Stress Response: Current Understanding and Future Directions
Authors: Rosenkranz Remus R. E. , Ullrich Sarah , Löchli Karin , Simm Stefan , Fragkostefanakis Sotirios
Published: June 2022

Alternative splicing (AS) is a major mechanism for gene expression in eukaryotes, increasing proteome diversity but also regulating transcriptome abundance. High temperatures have a strong impact on the splicing profile of many genes and therefore AS is considered as an integral part of heat stress response. While many studies have established a detailed description of the diversity of the RNAome under heat stress in different plant species and stress regimes, little is known on the underlying mechanisms that control this temperature-sensitive process. AS is mainly regulated by the activity of splicing regulators. Changes in the abundance of these proteins through transcription and AS, post-translational modifications and interactions with exonic and intronic cis-elements and core elements of the spliceosomes modulate the outcome of pre-mRNA splicing. As a major part of pre-mRNAs are spliced co-transcriptionally, the chromatin environment along with the RNA polymerase II elongation play a major role in the regulation of pre-mRNA splicing under heat stress conditions. Despite its importance, our understanding on the regulation of heat stress sensitive AS in plants is scarce. In this review, we summarize the current status of knowledge on the regulation of AS in plants under heat stress conditions. We discuss possible implications of different pathways based on results from non-plant systems to provide a perspective for researchers who aim to elucidate the molecular basis of AS under high temperatures.
Using a Hybrid Mapping Population to Identify Genomic Regions of Pyrenophora teres Associated With Virulence
Authors: Dahanayaka Buddhika A. , Snyman Lislé , Vaghefi Niloofar , Martin Anke
Published: June 2022

Net blotches caused by Pyrenophora teres are important foliar fungal diseases of barley and result in significant yield losses of up to 40%. The two types of net blotch, net-form net blotch and spot-form net blotch, are caused by P. teres f. teres (Ptt) and P. teres f. maculata (Ptm), respectively. This study is the first to use a cross between Ptt and Ptm to identify quantitative trait loci (QTL) associated with virulence and leaf symptoms. A genetic map consisting of 1,965 Diversity Arrays Technology (DArT) markers was constructed using 351 progenies of the Ptt/Ptm cross. Eight barley cultivars showing differential reactions to the parental isolates were used to phenotype the hybrid progeny isolates. Five QTL associated with virulence and four QTL associated with leaf symptoms were identified across five linkage groups. Phenotypic variation explained by these QTL ranged from 6 to 16%. Further phenotyping of selected progeny isolates on 12 more barley cultivars revealed that three progeny isolates are moderately to highly virulent across these cultivars. The results of this study suggest that accumulation of QTL in hybrid isolates can result in enhanced virulence.
Soil Respiration of Paddy Soils Were Stimulated by Semiconductor Minerals
Authors: Bai Yinping , Nan Ling , Wang Qing , Wang Weiqi , Hai Jiangbo , Yu Xiaoya , Cao Qin , Huang Jing , Zhang Rongping , Han Yunwei , Yang Min , Yang Gang
Published: June 2022

Large quantities of semiconductor minerals on soil surfaces have a sensitive photoelectric response. These semiconductor minerals generate photo-electrons and photo-hole pairs that can stimulate soil oxidation–reduction reactions when exposed to sunlight. We speculated that the photocatalysis of semiconductor minerals would affect soil carbon cycles. As the main component of the carbon cycle, soil respiration from paddy soil is often ignored. Five rice cropping areas in China were chosen for soil sampling. Semiconductor minerals were measured, and three main semiconductor minerals including hematile, rutile, and manganosite were identified in the paddy soils. The identified semiconductor minerals consisted of iron, manganese, and titanium oxides. Content of Fe2O3, TiO2, and MnO in the sampled soil was between 4.21–14%, 0.91–2.72%, and 0.02–0.22%, respectively. Most abundant semiconductor mineral was found in the DBDJ rice cropping area in Jilin province, with the highest content of Fe2O3 of 14%. Soils from the five main rice cropping areas were also identified as having strong photoelectric response characteristics. The highest photoelectric response was found in the DBDJ rice cropping area in Jilin province with a maximum photocurrent density of 0.48 μA/cm2. Soil respiration was monitored under both dark and light (3,000 lux light density) conditions. Soil respiration rates in the five regions were (from highest to lowest): DBDJ > XNDJ > XBDJ > HZSJ > HNSJ. Soil respiration was positively correlated with semiconductor mineral content, and soil respiration was higher under the light treatment than the dark treatment in every rice cropping area. This result suggested that soil respiration was stimulated by semiconductor mineral photocatalysis. This analysis provided indirect evidence of the effect semiconductor mineral photocatalysis has on the carbon cycle within paddy soils, while exploring carbon conversion mechanisms that could provide a new perspective on the soil carbon cycle.
Can Organizational Identification Weaken the Negative Effects of Customer Bullying?—Testing the Moderating Effect of Organizational Identification
Authors: Huang Haili , Yu Shengxian , Peng Pin
Published: June 2022

Customer bullying is a common phenomenon, causing short-term emotional distress or having long-term psychological impact on frontline employees of service enterprises, yielding either direct or indirect losses to service enterprises. While existing research has focused on the emotional and psychological impact of customer bullying on employees, little attention has been directed at the impact of customer bullying on negative employee behavior and internal mechanisms. In view of this, this paper draws on conservation of resources theory and discusses how and when customer bullying can lead to unethical behaviors. Furthermore, the mediating role of job insecurity and the moderating effect of organizational identification are analyzed. In study 1, 181 valid questionnaire data were collected at two time points, and regression data analysis was used to explore the effect of customer bullying on unethical behaviors through job insecurity. In study 2, 212 employees were recruited to investigate the moderating effect of organizational identification between customer bullying and unethical behaviors through a scenario experimental study. The results reveal that customer bullying is positively related to employees’ job insecurity and unethical behaviors. Job insecurity partially mediates the positive relationship between customer bullying and unethical behaviors. Further, the regression analysis results indicate that the direct effect of customer bullying on unethical behaviors is moderated by organizational identification. This study provides theoretical guidance for entrepreneurs to reduce both employee job insecurity and unethical behavior.
Reverse-Coded Items Do Not Work in Spanish: Data From Four Samples Using Established Measures
Authors: Venta Amanda , Bailey Cassandra A. , Walker Jesse , Mercado Alfonso , Colunga-Rodriguez Cecilia , Ángel-González Mario , Dávalos-Picazo Gabriel
Published: June 2022

The potential for suboptimal psychometric performance of reverse-coded items may be particularly pronounced when scales are translated and administered in Spanish with these problems exacerbated in youth respondents. This is a significant concern, given the rapid rise in Hispanic-American and Spanish-speaking individuals in the US and their rightful, growing representation in psychological research and clinical care. The aim of this study was to examine the psychometric performance of reverse-coded items across four Spanish-speaking samples spanning developmental stages including youth, college students, and parents (N = 1,084; Adolescents n = 107; M = 19.79; SD = 2.09; 41.1% female; Caregivers n = 58; M = 40.79; SD = 7.94; 60.3% female; Spanish-speaking adults in the US n = 157; M = 33.4; SD = 9.5; 68.8% female; and College students living in Latin America n = 783; M = 21.04; SD = 3.13; 69.2% female) and four scales (Big Five Inventory; Strengths and Difficulties Questionnaire; Difficulties in Emotion Regulation Scale; Beck Hopelessness Scale); we expected reverse-coded items would demonstrate inadequate item–total correlations and their inclusion would compromise scale internal consistency. Hypotheses were supported with evidence of poor psychometric performance for at least two reverse-coded items on each instrument, such that un-reversing the items improved their item–total correlations. Across every instrument, alpha was either improved by excluding reverse-coded items or by including them in an un-reversed fashion and, overall, there was a moderate, negative effect of reverse-coded items on scale alphas. In growing consensus with previous authors, we recommend that reverse-coded items not be included in Spanish scales.
Psychometric Properties of ADHD Rating Scale—5 for Children and Adolescents in Sudan—School Version
Authors: Alhossein Abdulkarim , Abaoud Abdulrahman Abdullah , Becker David , Aldabas Rashed , Bakhiet Salaheldin Farah , Al Jaffal Mohammed , Alsufyani Manar , Abdu Elrahim Nagda Mohamed , Alzrayer Nouf
Published: June 2022

The ADHD Rating Scale—5 for Children and Adolescents, School Version, has been adopted and validated to be used in assessing ADHD among school children within Western contexts. However, there are few assessment tools in use for identifying ADHD characteristics in children in Sudan. Therefore, this study aimed to investigate the psychometric properties of this rating scale in the context of Sudan. To accomplish this, data were collected on a sample of 3,742 school-aged children and adolescents as reported by their teachers. Psychometric properties can be classified as very good, with very high reliability (>0.90), and high construct validity tested by exploratory and confirmatory factor analysis. Thus, the ADHD Rating Scale—5 for Children and Adolescents, School Version, is valid, reliable, and suitable to use for assessing ADHD symptoms among children and adolescents in the Sudanese context.
Choosing Sides: Impact of Prismatic Adaptation on the Lateralization of the Attentional System
Authors: Clarke Stephanie , Farron Nicolas , Crottaz-Herbette Sonia
Published: June 2022

Seminal studies revealed differences between the effect of adaptation to left- vs. right-deviating prisms (L-PA, R-PA) in normal subjects. Whereas L-PA leads to neglect-like shift in attention, demonstrated in numerous visuo-spatial and cognitive tasks, R-PA has only minor effects in specific aspects of a few tasks. The paucity of R-PA effects in normal subjects contrasts with the striking alleviation of neglect symptoms in patients with right hemispheric lesions. Current evidence from activation studies in normal subjects highlights the contribution of regions involved in visuo-motor control during prism exposure and a reorganization of spatial representations within the ventral attentional network (VAN) after the adaptation. The latter depends on the orientation of prisms used. R-PA leads to enhancement of the ipsilateral visual and auditory space within the left inferior parietal lobule (IPL), switching thus the dominance of VAN from the right to the left hemisphere. L-PA leads to enhancement of the ipsilateral space in right IPL, emphasizing thus the right hemispheric dominance of VAN. Similar reshaping has been demonstrated in patients. We propose here a model, which offers a parsimonious explanation of the effect of L-PA and R-PA both in normal subjects and in patients with hemispheric lesions. The model posits that prismatic adaptation induces instability in the synaptic organization of the visuo-motor system, which spreads to the VAN. The effect is lateralized, depending on the side of prism deviation. Successful pointing with prisms implies reaching into the space contralateral, and not ipsilateral, to the direction of prism deviation. Thus, in the hemisphere contralateral to prism deviation, reach-related neural activity decreases, leading to instability of the synaptic organization, which induces a reshuffling of spatial representations in IPL. Although reshuffled spatial representations in IPL may be functionally relevant, they are most likely less efficient than regular representations and may thus cause partial dysfunction. The former explains, e.g., the alleviation of neglect symptoms after R-PA in patients with right hemispheric lesions, the latter the occurrence of neglect-like symptoms in normal subjects after L-PA. Thus, opting for R- vs. L-PA means choosing the side of major IPL reshuffling, which leads to its partial dysfunction in normal subjects and to recruitment of alternative or enhanced spatial representations in patients with hemispheric lesions.
Morbidities Worsening Index to Sleep in the Older Adults During COVID-19: Potential Moderators
Authors: Almondes Katie Moraes de , Castro Eleni de Araujo Sales , Paiva Teresa
Published: June 2022

Older adults were considered a vulnerable group for the COVID-19 infection and its consequences, including problems with sleep.

Aim

To evaluate the prevalence of sleep disorders in older adults, to describe their sleep patterns, as well as to analyse if there were any changes in comparison with the period pre-pandemic.

Materials and Methods

Online survey used for data collection received answers from 914 elderly age range 65–90 years, from April to August 2020. Results: 71% of the sample reported a pre-existent sleep disorder, and some of them worsened during the pandemic, especially Insomnia in women and Obstructive Sleep Apnea in men. No difference in sleep duration before and during the pandemic was found, although there was a worsening of some aspects related to sleep, such as sleep quality, sleep efficiency, awakening quality, sleep latency and nocturnal awakenings, especially in the female gender. Educational level influenced sleep latency, indicating higher sleep latency among those with primary education when compared with the ones with Ph.D.

Conclusion

The pandemic had influenced sleep patterns among the elderly, as well as worsening of pre-existent sleep disorders. Female gender and low educational level were considered risk factors for sleep alterations, and high educational level, on its turn, appeared to be a protective factor.
Perception, Beliefs, and Causal Attribution of Autism Early Signs in Ecuadorian General Population
Authors: Buffle Paulina , Gentaz Edouard , Vivanti Giacomo
Published: June 2022

The identification and diagnosis of children with autism currently rely on behavioral presentation and developmental history. Cultural norms and other socio-demographic factors can influence what is expected or non-expected behaviors in a developing child. Perceptions, beliefs, and causal attribution of early signs can influence families’ help-search behaviors. Lack of recognition of autism’s first manifestations can critically delay the age of diagnosis, the provision of informed guidance to families, and the implementation of adapted interventions during the critical period of early development. Furthermore, a lack of understanding of early signs as the manifestations of a developmental condition may increase stigma and non-conventional explanations. Still, cultural and socio-demographic factors are largely understudied, particularly in low-and middle-income settings. Based on the hypothesis that non-specialists such as family members and friends are one of the first sources of referral in Latin American contexts, we aimed to study the general population’s perceptions and the explanatory causes of autism’s early signs. One-hundred-and-eighty-three Ecuadorian adults responded to a questionnaire developed for this study, describing sixteen ASD-related behaviors. Results indicated that, with the exemption of language impairment and self-injurious behaviors, a substantial proportion of participants did not endorse many behaviors as “concerning and requiring professional attention.” Also, language impairment was the only behavior identified as related to a developmental disorder. Additionally, most participants attributed the majority of behaviors listed in the questionnaire to causes unrelated to ASD, such as child personality. We discuss the impact of those findings in clinical practice and on awareness programs.
A Study on the Relationship Between Consumer Motivations and Subjective Well-Being: A Latent Profile Analysis
Authors: Xiao Jun , Gong Yanping , Li Jian , Tang Xiuyuan , Javeed Sohail Ahmad , Deng Yuling
Published: June 2022

Consumer motivation plays an important role in their purchase decisions and well-being. Previous studies have investigated the relationship between certain consumer motivations and well-being separately. We aim to employ latent profile analysis (LPA) to explore subgroups of participants who display similar patterns of consumer multiple motivations and to examine differences in subjective well-being across these subtypes. The final group of (N = 1,023, ages 18–58) completed the Consumer Motivation scale online, assessing seven dimensions of consumer motivation. Results of LPA identified four subpopulations of participants: the enthusiastic group has high expectations in all aspects; the balanced group values each aspect of the product but has lower expectations; the rational group emphasizes aspects such as value for money, comfort, and quality; the apathetic group has no strong motivation for consumption, and they are relatively concerned with the dimensions of security, social acceptance, and stimulation. Consumers’ subjective well-being (life satisfaction, positive affect, and negative affect) differed significantly across the four profiles. Specifically, scores for positive affect and life satisfaction were highest in the enthusiastic group, medium in the balanced group, and lowest in the rational and apathetic groups. Scores for negative affect were significantly higher in the enthusiastic and apathetic groups than in the rational group. These findings enable marketers to develop customized marketing strategies for different motivation profiles and contribute to helping consumers with varying motivation profiles to consciously pay attention to their well-being.
Social Touch, Social Isolation, and Loneliness in Borderline Personality Disorder During the COVID-19 Pandemic
Authors: Schulze Anna , Biermann Miriam , Atanasova Konstantina , Unterseher Franziska , Winkler Louisa , Bohus Martin , Lis Stefanie
Published: June 2022

Background

Interpersonal impairments in borderline personality disorder (BPD) are characterised by a lack in the sense of belonging and the fear of being excluded. One feature of interactions that can promote a sense of social belonging is interpersonal touch. While some studies suggest that individuals with BPD experience social touch as less pleasurable than healthy individuals (HCs), there are no studies that investigated whether this difference is associated with feeling less socially connected. This question is particularly important during the COVID-19 pandemic, since one central behavioural recommendation is “social distancing”. An increase in loneliness has been discussed as a consequence and it has been suggested that individuals with BPD may be particularly burdened. However, the primary goal of “social distancing” is not preventing social contacts, but physical proximity. In our study we investigated the interplay between feeling close to others, contact frequency and the appraisal of social touch in BPD. We were additionally interested in whether these factors contribute to the burden through “physical distancing”.

Methods

We assessed subjective and objective social isolation, the need, importance, and liking of social touch, as well as the burden through “physical distancing” policies in 130 women (61 BPD and 69 HCs).

Results

Participants of the BPD group reported higher loneliness, less social contacts and a lower need for, importance and liking of social touch compared to HCs. Larger social networks, higher frequency of in-person contacts and higher liking and importance of social touch were associated with lower levels of loneliness. Both groups did not differ regarding their burden through “physical distancing”. A higher need for and lower importance of social touch predicted a higher burden through “physical distancing”.

Conclusions

A positive appraisal of social touch was associated with less loneliness, independently of an individual's objective social isolation. In BPD, impairments of this fundamental facet of social interaction might hamper forming and strengthening of social bonds and contribute to the patients' interpersonal dysfunction. Changing the attitude towards social touch and in consequence its liking and importance in social interaction might provide one avenue to improve the sense of social connectedness in these patients.
Effects of Persistent Binge Drinking on Brain Structure in Emerging Adults: A Longitudinal Study
Authors: Pérez-García Jose Manuel , Cadaveira Fernando , Canales-Rodríguez Erick J. , Suárez-Suárez Samuel , Rodríguez Holguín Socorro , Corral Montserrat , Blanco-Ramos Javier , Doallo Sonia
Published: June 2022

Previous cross-sectional research has largely associated binge drinking (BD) with changes in volume and thickness during adolescence and early adulthood. Nevertheless, the long-term alcohol-related effects on gray matter features in youths who had maintained a BD pattern over time have not yet been sufficiently explored. The present study aimed to assess group differences both cross-sectionally and longitudinally [using symmetric percent change (SPC)] on several structural measures (i.e., thickness, surface area, volume). For this purpose, magnetic resonance imaging was recorded twice within a 2-year interval; at baseline (18–19 years) and a follow-up (20–21 years). The sample included 44 university students who were classified as 16 stable binge drinkers (8 females) and 28 stable controls (13 females). Whole-brain analysis showed larger insular surface area in binge drinkers relative to controls at follow-up (cluster-wise p = 0.045). On the other hand, region of interest (ROI) analyses on thickness also revealed a group by sex interaction at follow-up (p = 0.005), indicating that BD males had smaller right rostral middle frontal gyrus thickness than both control males (p = 0.011) and BD females (p = 0.029). Similarly, ROI-based analysis on longitudinal data showed a group by sex interaction in the right nucleus accumbens (p = 0.009) which revealed a decreased volume across time in BD males than in control males (p = 0.007). Overall, continued BD pattern during emerging adulthood appears to lead to gray matter abnormalities in regions intimately involved in reward processing, emotional regulation and executive functions. Notably, some anomalies varied significantly depending on sex, suggesting a sex-specific impact of BD on typical neurodevelopment processes.
Interpreting Deep Machine Learning for Streamflow Modeling Across Glacial, Nival, and Pluvial Regimes in Southwestern Canada
Authors: Anderson Sam , Radić Valentina
Published: June 2022

The interpretation of deep learning (DL) hydrological models is a key challenge in data-driven modeling of streamflow, as the DL models are often seen as “black box” models despite often outperforming process-based models in streamflow prediction. Here we explore the interpretability of a convolutional long short-term memory network (CNN-LSTM) previously trained to successfully predict streamflow at 226 stream gauge stations across southwestern Canada. To this end, we develop a set of sensitivity experiments to characterize how the CNN-LSTM model learns to map spatiotemporal fields of temperature and precipitation to streamflow across three streamflow regimes (glacial, nival, and pluvial) in the region, and we uncover key spatiotemporal patterns of model learning. The results reveal that the model has learned basic physically-consistent principles behind runoff generation for each streamflow regime, without being given any information other than temperature, precipitation, and streamflow data. In particular, during periods of dynamic streamflow, the model is more sensitive to perturbations within/nearby the basin where streamflow is being modeled, than to perturbations far away from the basins. The sensitivity of modeled streamflow to the magnitude and timing of the perturbations, as well as the sensitivity of day-to-day increases in streamflow to daily weather anomalies, are found to be specific for each streamflow regime. For example, during summer months in the glacial regime, modeled daily streamflow is increasingly generated by warm daily temperature anomalies in basins with a larger fraction of glacier coverage. This model's learning of “glacier runoff” contributions to streamflow, without any explicit information given about glacier coverage, is enabled by a set of cell states that learned to strongly map temperature to streamflow only in glacierized basins in summer. Our results demonstrate that the model's decision making, when mapping temperature and precipitation to streamflow, is consistent with a basic physical understanding of the system.
A Novel BRD Family PROTAC Inhibitor dBET1 Exerts Great Anti-Cancer Effects by Targeting c-MYC in Acute Myeloid Leukemia Cells
Authors: Zhang Kunlong , Gao Li , Wang Jianwei , Chu Xinran , Zhang Zimu , Zhang Yongping , Fang Fang , Tao Yanfang , Li Xiaolu , Tian Yuanyuan , Li Zhiheng , Sang Xu , Ma Li , Lu Lihui , Chen Yanling , Yu Juanjuan , Zhuo Ran , Wu Shuiyan , Pan Jian , Hu Shaoyan
Published: June 2022

Acute myeloid leukemia (AML) represents an aggressive hematopoietic malignancy with a prognosis inferior to that of other leukemias. Recent targeted therapies offer new opportunities to achieve better treatment outcomes. However, due to the complex heterogeneity of AML, its prognosis remains dismal. In this study, we first identified the correlation between high expression of BRD4 and overall survival of patients with AML. Targeted degradation of BRD2, BRD3, and BRD4 proteins by dBET1, a proteolysis-targeting chimera (PROTAC) against the bromodomain and extra-terminal domain (BET) family members, showed cytotoxic effects on Kasumi (AML1-ETO), NB4 (PML-RARa), THP-1 (MLL-AF9), and MV4-11 (MLL-AF4) AML cell lines representing different molecular subtypes of AML. Furthermore, we determined that dBET1 treatment arrested cell cycling and enhanced apoptosis and c-MYC was identified as the downstream target. Collectively, our results indicated that dBET1 had broad anti-cancer effects on AML cell lines with different molecular lesions and provided more benefits to patients with AML.
Foot Drop Stimulation via Piezoelectric Energy Harvester
Authors: Soozandeh Parham , Poudel Ganga , Sarkari Morteza , Behdinan Kamran
Published: June 2022

The design and implementation of a piezoelectric energy-harvesting system, aimed at stimulating the Tibialis anterior muscle to aid patients struggling with a foot drop disability, are investigated. A physical prototype designed to be installed inside a shoe sole, consisting of an energy-harvesting unit along with a power-management circuit and a functional electrical-stimulation circuit, is fabricated. The piezoelectric energy harvester (PEH) incorporated six layers of Polyvinylidene-Fluoride sheets to achieve a mean-charge generation of 65.25 μC/step and a peak power of 10.76 mW/step. A peak voltage of +80.0 V generation was achieved during a stomping motion. The electrical systems store, convert, and deploy 60 mA electric pulses at the desired frequencies to the target muscle. The finalized prototype is best-suited to prolong the duration of the charged batteries whilst in use. In a practical sense, it should be used alongside external-power sources to recharge the batteries installed in a foot drop stimulation device. The PEH in its current state is fully capable of solely powering blood pressure sensors, glucose meters, or activity trackers.
Adaptive Synergetic Motion Control for Wearable Knee-Assistive System: A Rehabilitation of Disabled Patients
Authors: Mahdi Shaymaa M. , Yousif Noor Q. , Oglah Ahmed A. , Sadiq Musaab E. , Humaidi Amjad J. , Azar Ahmad Taher
Published: June 2022

In this study, synergetic-based adaptive control design is developed for trajectory tracking control of joint position in knee-rehabilitation system. This system is often utilized for rehabilitation of patients with lower-limb disabilities. However, this knee-assistive system is subject to uncertainties when applied to different persons undertaking exercises. This is due to the different masses and inertias of different persons. In order to cope with these uncertainties, an adaptive scheme has been proposed. In this study, an adaptive synergetic control scheme is established, and control laws are developed to ensure stable knee exoskeleton system subjected to uncertainties in parameters. Based on Lyapunov stability analysis, the developed adaptive synergetic laws are used to estimate the potential uncertainties in the coefficients of the knee-assistive system. These developed control laws guarantee the stability of the knee rehabilitation system controlled by the adaptive synergetic controller. In this study, particle swarm optimization (PSO) algorithm is introduced to tune the design parameters of adaptive and non-adaptive synergetic controllers, in order to optimize their tracking performances by minimizing an error-cost function. Numerical simulations are conducted to show the effectiveness of the proposed synergetic controllers for tracking control of the exoskeleton knee system. The results show that compared to classical synergetic controllers, the adaptive synergetic controller can guarantee the boundedness of the estimated parameters and hence avoid drifting, which in turn ensures the stability of the controlled system in the presence of parameter uncertainties.
Potato Zero-Tillage and Mulching Is Promising in Achieving Agronomic Gain in Asia
Authors: Ramírez David A. , Silva-Díaz Cecilia , Ninanya Johan , Carbajal Mariella , Rinza Javier , Kakraliya Suresh K. , Gatto Marcel , Kreuze Jan
Published: June 2022

Rice-based systems have recently been recognized as the most critical plant source of C emissions worldwide. Globally, rice production is highest in Asia. Actions to introduce sustainable intensification practices into existing rice lands or diversifying with lower C-emitting crops such as potatoes will be crucial to mitigate climate change. The objective of this study is to analyze the effect of potato cultivation under zero/minimum-tillage and/or organic mulching (with emphasis on rice-straw) (PZTM) on key performance indicators that are crucial to achieving agronomic gains in Asia. Forty-nine studies were selected and systematically reviewed to address the study objective. Studies reveal a consensus of increase in yield, profitability, nutrient-use efficiency, and water productivity, promoted by the significant soil moisture conservation in PZTM. There is inconsistent evidence that zero-tillage benefits weed control, but its effectiveness is enhanced by mulching. Even if soil organic matter is increased (+13–33%) and zero-tillage is the main factor driving the reduction in C footprint, no values of kg CO2 eqha−1 have been reported in PZTM to date. Only a small fraction (∼2%) of the rice-cultivated areas (RCA) is intensified with potato cultivation. That way, scaling-up PZTM among rice farmers has large potential (∼24% RCA) to increase the sustainable intensification of rice-based systems in Asia.