bluuebunny/crossref_metadata_2025 huggingface dataset:
QUANDO UM PAPEL AMASSADO ATRAVESSA A SALA DE AULA: EXPERIENCIANDO A SEXUALIDADE COM ALUNOS/AS DO ENSINO FUNDAMENTAL
Authors: Amorim Graziele Corrêa , Hernek Heloísa Raimunda
Published: October 2018

A instituição escolar compõe-se de uma multiplicidade de modos de pensar, agir, sentir e arquitetar o viver. Passar, pois, a habitá-la nos propicia o convívio com um emaranhado de temáticas que emergem inesperadamente. Assim, dentre as problemáticas que podem surgir, apresentamos o interesse de discutir, com o cotidiano escolar, as sexualidades dos/das estudantes. Desse modo, o ponto de partida para essa discussão são as narrativas produzidas e experienciadas por uma professora iniciante, com seus alunos de uma turma de terceiro ano do ensino fundamental.
INTRODUÇÃO DO DEBATE SOBRE GÊNERO E DIVERSIDADE NO IFCE CAMPUS CRATO: OS PRIMEIROS PASSOS
Authors: Pereira Lorena Kelly Alves , Fernandes Francisete Pereira
Published: October 2018

Este artigo tem por objetivo relatar os primeiros passos do surgimento do debate sobre gênero e diversidade no Instituto Federal de Educação, Ciência e Tecnologia do Ceará – IFCE campus Crato a partir da construção coletiva de um evento que, posteriormente, passou a fazer parte da política de atuação da escola em relação a esses temas. Trata-se do evento chamado Encontro sobre Diversidade.
Interseccionalidade, gênero, sexualidade e raça: os desafios e as potencialidades na invenção de outros currículos
Authors: OLIVEIRA DANILO ARAUJO DE , FERRARI ANDERSON
Published: October 2018

O conceito de interseccionalidade é tomado como aposta na problematização do sujeito na sua relação com o saber e currículo. Para isso, é a escola e o investimento na sala de aula que é o foco da nossa análise. Quais os desafios e as potencialidades de uma aula de história pensando o corpo negro? Essa é a questão que organizou a preparação de uma aula de história de um professor negro e gay interessado em colocar sob suspeita a hegemonia branca e heterossexual na constituição histórica do brasileiro.Palavras-chave: Interseccionalidade; Gênero; Sexualidade; Raça.
A aceitação da terapia homeopática em hospitais da Universidade Federal do Rio de Janeiro
Authors: Iozzi Giovanna Menezes , Da Silva Rosanna Iozzi , Homsani Fortune Homsani , Oliveira Adriana Passos , Capella Marcia Alves Marques , Holandino Carla
Published: October 2018

Nos últimos dez anos, tem-se verificado o aumento do interesse pela terapia homeopática devido a sua inclusão no SUS, oficializada na "Política Nacional de Práticas Integrativas e Complementares” (PNPIC). A Universidade Federal do Rio de Janeiro (UFRJ) possui dois hospitais universitários de grande importância no Sistema Único de Saúde (SUS): o Hospital Universitário Clementino Fraga Filho (HUCFF) e o Instituto de Puericultura e Pediatria Martagão Gesteira (IPPMG). O presente estudo avaliou, ao todo, a opinião de 142 médicos, sendo 110 do HUCFF e 32 do IPPMG, sobre a homeopatia, através de um questionário específico. Após coleta e tratamento estatístico dos dados, os resultados indicaram que apenas 14 médicos já tinham indicado e/ou utilizado a terapia homeopática anteriormente, apesar da inexistência de ambulatório de homeopatia em ambos os hospitais avaliados. A análise dos questionários evidenciou, também, que a maioria dos médicos entrevistados, além de desconhecerem a terapia homeopática, acham irrelevante que o serviço de homeopatia seja oferecido nesses hospitais. Dessa forma, verificamos a necessidade de inclusão da homeopatia na graduação de medicina da UFRJ com a ampliação do currículo dos médicos da UFRJ, como já é feito na UNIRIO e na UFF, e de mapear a percepção dos profissionais da saúde acerca da homeopatia, fornecendo dados que favoreceram a implantação da homeopatia na Atenção Básica à Saúde, como previsto na PNPIC.
Avaliação da produção de drogas vegetais, derivados vegetais e fitoterápicos do laboratório de manipulação farmacêutica para fornecimento ao Programa de Fitoterapia e Homeopatia da Secretaria Municipal da Saúde de Ribeirão Preto – SP
Authors: Borella Júlio Cézar , Teixeira Juliana Coelho Lopes
Published: October 2018

O Programa de Fitoterapia e Homeopatia da Secretaria da Saúde de Ribeirão Preto foi um dos primeiros a atuar no estado de São Paulo, a partir de 1992. A demanda de produtos a base de plantas medicinais para este programa foi suprida pelo trabalho do Laboratório de Manipulação Farmacêutica. A partir dos dados relativos à produção deste setor, nos anos de atuação do programa (1994 até 2017), foi possível usar os resultados como parâmetro avaliativo da atuação deste programa no município de Ribeirão Preto - SP. Observou-se a existência de fases na atuação do programa, sendo a primeira (1994 a 2002) que se caracterizou pela diversidade de preparações (21 tipos) e um clínico com direito a prescrição. Nesta fase houve a produção de 4.318 unidades medicamentosas, com maior demanda para Calendula officinalis pomada 10% (737), Maytenus ilicifolia folhas (424), Passiflora alata tintura 20% (321) e Allium sativum cápsulas 500mg (314). Outra fase (2003 a 2015) teve como características a drástica diminuição da diversidade de preparações [2 tipos - Calendula officinalis pomada 10% (29.779) e Stryphnodendron adstringens creme a 10% (1.532)] e o aumento do número de unidades produzidas (31.311 unidades), pela possibilidade de indicação de uso ter sido ampliada para integrantes de equipes multiprofissionais da rede de saúde do município. Nos anos de 2016 e 2017, o Laboratório de Manipulação Farmacêutica passou por séria crise produtiva que estava associada à crise econômica e política, com consequente restrição na produção de itens para uso no programa.
Prescrição fitoterápica por nutricionistas: percepção e adequação à prática
Authors: Luna Siqueira Ana Bolena , Martins René Duarte
Published: October 2018

O aumento na utilização de plantas medicinais, através da fitoterapia tem crescido na população ampliando a prescrição por profissionais da área de saúde. Após a autorização do Conselho Federal de Nutrição, através da resolução número 402/2007, os nutricionistas agregaram os fitoterápicos ao tratamento dietoterápico de forma complementar. O objetivo deste foi caracterizar a prescrição fitoterápica por nutricionistas e seus sentimentos frente à esta habilitação. Adotou-se um desenho qualitativo, utilizando roteiro de entrevistas semiestruturado, aplicado aos nutricionistas atuantes na capital de Pernambuco, através da técnica “bola de neve” e amostragem por critério de saturação da informação. A análise de conteúdo dos depoimentos foi feita a partir do modelo de categorias temáticas de Bardin. Verificou-se que boa parte dos entrevistados concluiu a graduação de Nutrição há pelo menos 11 anos, metade não realizou curso de aperfeiçoamento em fitoterapia, mas a maioria realiza prescrição para os casos de obesidade, como moderador de apetite, ansiedade entre outros. Os motivos para prescreverem ou não, vão desde a falta de conhecimento, passando pela ausência do tema na graduação até a credibilidade do produto, envolvendo sentimentos de segurança e outras inquietações. Conclui-se que alguns integrantes do grupo estudado não estão preparados para prescrever com segurança. Os dados sugerem uma possível confusão quanto à prescrição e indicação, conforme resoluções nº 402/2007 e nº 525/2013, e pouca nitidez quanto à adequada prescrição na prática. Tornam-se importantes mais estudos sobre a prescrição de fitoterápicos pelos nutricionistas, pois investigará, após o conhecimento e aprovação de resoluções mais recentes, a prática, adequação e percepção desses profissionais. Palavras chave/constructos: Fitoterapia. Plantas medicinais. Habilidade. Pesquisa qualitativa
O vitalismo das práticas integrativas e complementares e o conceito de campo da ciência moderna
Authors: Abreu Isa Paula Hamouche
Published: October 2018

As Práticas Integrativas e Complementares (PICs) abrangem procedimentos de sistemas médicos complexos (que possuem sua própria racionalidade médica) e recursos terapêuticos, integrativos e vitalistas. São indicadas como terapêutica complementar ou principal em diversas condições clínicas e nosológicas. Entretanto, estas práticas terapêuticas permanecem marginalizadas perante a racionalidade científica vigente, por estarem fundamentadas em paradigmas pouco ortodoxos, que desafiam o pensamento cartesiano-mecanicista ainda predominante. As PICs, de um modo geral, estão embasadas em uma concepção holística e vitalista, compreendendo o ser humano como uma unidade físico-energética/psicofísica, mental e espiritual, apresentando, portanto, uma dimensão material (corpo) e uma dimensão imaterial, sutil, que não pode ser apreendida pelos cinco sentidos. Por sua vez, a influência do pensamento cartesiano-mecanicista no campo das ciências da saúde resultou no chamado modelo biomédico, que se caracteriza pela dicotomia corpo-mente, pelo enfoque na doença e não na pessoa doente, pelo emprego exclusivo do método analítico, ou seja, da parte sem consideração da totalidade e do contexto, em síntese, pelo reducionismo e fragmentação em detrimento de uma visão sistêmica e integral. Todavia, os avanços da ciência moderna, particularmente nos campos da física e da psicologia, provocaram um rompimento com as ideias tradicionais a respeito do universo, da matéria e do ser humano. Este artigo tem por objetivo demonstrar que muitos dos avanços e teorias científicas da modernidade podem auxiliar na compreensão das PICs, enfocando particularmente as conexões da teoria vitalista com o eletromagnetismo e a teoria de campo.
Corrente interferencial e terapia manual reduzem sinais e sintomas de dor lombar crônica
Authors: Araújo Antonio Carlos Pereira de , Felber Daniel Tassinari , Yamada Eloá Ferreira , Teixeira Lilian Pinto , Da Silva Morgana Duarte
Published: October 2018

Objetivo: verificar o efeito da corrente interferencial associada a técnicas manuais sobre a dor, a funcionalidade, a qualidade de vida e aspectos depressivos em indivíduos com dor lombar crônica. Metodologia: este estudo caracteriza-se como pesquisa experimental, através de uma observação sistemática de um único grupo experimental. Quatorze indivíduos com dor lombar crônica foram submetidos a um protocolo composto por 10 sessões de eletroterapia utilizando corrente interferencial e terapia manual. Para a coleta de dados foi utilizada uma ficha de avaliação fisioterapêutica constituída por anamnese, inspeção, escala visual de dor (EVA), teste de Shober, questionário SF-36, questionário de Oswestry e questionário de Depressão de Beck. Os dados foram analisados por teste t com teste de Wilcoxon, com nível de significância p
XXIV Semana Acadêmica do Curso de Medicina da Universidade Federal do Rio Grande: “vivenciando novas perspectivas, abrindo novos horizontes”
Authors: Medicina Faculdade de
Published: October 2018

A XXIV Semana Acadêmica do Curso de Medicina da Universidade Federal do Rio Grande – FURG (SAMED), organizada pelo Diretório Acadêmico de Medicina Francisco Martins Bastos (DAFB), ocorreu nos dias 18, 19 e 20 de abril de 2018 e teve como público-alvo os graduandos do curso de Medicina da FURG. O evento ocorre bianualmente e em 2018 teve como Coordenadora Geral a Professora Marilice Magroski Gomes da Costa, Coordenadora Acadêmica, a Professora Vera Regina Mendonça Signorini, e Coordenador Científico, o Professor Samuel de Carvalho Dumith – todos docentes da Faculdade de Medicina da FURG.
PL-002 AGTR1 polymorphism is associated with elite endurance athletes: A functional study
Authors: Yang Xiaolin , Hu Yang , Li Yanchun , Mei Tao , Gong Lijing
Published: October 2018

Objective To explore the association between the polymorphism of angiotensin II receptor type 1 gene(AGTR1) and elite endurance athlete performance and the mechanism of how the polymorphism works.

Methods (1) Polymorphism of AGTR1 rs5182 between 122 elite Chinese endurance athletes and 222 controls were analyzed by MALDI-TOF-MS. (2) Aerobic capacity of 79 elite Chinese endurance athletes such as VC/FEV1/MVV/ VO2AT/ HRAT/ VAT/ VO2max/ HRmax/ VVO2max were measured and association between rs5182 polymorphism and the performance was analyzed. (3) PcDNA3.1-AGTR1 -T and pcDNA3.1-AGTR1-C plasmid were build and the plasmids was transfected into mammalian 293T cells. mRNA levels were detected after 48 hours. Statistical analysis was performed using SPSS software version 15.0. Values of P < 0.05 were considered statistically significant. Continuous data were expressed as mean ± SD, while categorical data were expressed as frequencies. Genotype distribution and allele frequencies between athletes and control subjects were compared using χ2 tests. Aerobic performance data was analyzed with One-Way ANOVA if it conformed to normal distribution and homogeneity of variance otherwise Non-parametric test of independent sample was used.

Results (1) Genotype frequencies of AGTR1 rs5182 are significant differences between the athletes and control subjects (p = 0.040), the Word-Class athletes and control subjects (p = 0.018), 5km athletes and control subjects (p =0.015), 10km athletes and control subjects (p = 0.026), male athletes and male controls(p=0.045). (2) Association is found between Genotype distribution and MV(L/min) though others not (Genotype: MV; CC: 122.514±6.767; CT:117.187±17.961; TT:119.688±20.226, p=0.047). (3) Transiently transfectedpcDNA3.1-AGTR1-T and pcDNA3.1-AGTR1-C plasmids into 293T cells successfully. The differences of mRNA levels between the groups were not significant (p = 0.991).

Conclusions  AGTR1 gene rs5182 could be a candidate genetic mark of selection elite endurance athletes in Han Population from Northern China, but this polymorphism does not affect AT1R protein function through changing its mRNA level.
PL - 023 Effects of Oleanolic Acid on Omega-3 and 6 Fatty Acids Metabolism in Rats fed with High Fructose Diet
Authors: Fuku Sandile
Published: October 2018

Objective The present study investigated the effect of OA on metabolism of omega 3 and 6 together with genes associated with lipid metabolism in high fructose diet fed Sprague Dawley rats. 

Methods Real Time PCR qPCR was conducted to evaluate gene expressions levels.

Gas Chromatography Mass Spectrometry (GS-MS): Lipid metabolism metabolites were assesed.

Measurement of gene expression using qPCR analysis: Expressions levels of glucose transport and lipid metabolism genes were assessed. 

Measurements of inflammatory markers concentration in Blood plasma:TNF-α and adiponectin concentrations were determined.

Results It was found that high fructose diet decreased gene ex pression levels of NRF-1 and GLUT-4 genes, however administration of OA increased expression levels of these genes.  Furthermore, adiponectin concentration was lowered in HFD group however this was reversed when HFD was accompanied by OA administration.  This increase in concentration of the anti-inflammatory molecule followed the increase in adiponectin receptor, AdipoR1 gene expression in skeletal muscle tissue. The results on lipid metabolism metabolites were consistent the observed molecular events, the anti-inflammatory signaling molecules (EPA and DHA) were five times higher in HF+OA than they were in HFD. High FAS,ACC-2 gene expression levels in HFD group, low 2 expression levels these genes together with increased CPT-1 in OA treated groups coincided with increased levels of arachidonic acid and linoleic acid levels in HFD, threefold increase compared to the control, and decreased levels in OA treated rats. 

Conclusions  Supplementation of diet with oleanolic acid produces DHA and EPA, thus modulating anti-inflammatory response and modulating effects of exercise in reversing diabetes.  We provided evidence to effect that diet has a therapeutic potential in managing metabolic syndrome related diseases.
Effects of HIITand MICT for 10 weeks on myocardial AMPK and PGC-1α in rats
Authors: Su Hao , Jiang Zhongye , Cao Guohuan , Wen Tianhao , Shao Jia , Li Liang
Published: October 2018

Objective: The improvement of cardiorespiratory fitness (CRF) is known as an effective strategy for prevention cardiovascular risk. Myocardial aerobic oxidation which control by the signal way of adenosine monophosphate -activated protein kinase (AMPK)- peroxisome proliferators γ activated receptor coativator-1-α (PGC-1α)  is the key for CRF. Previous studies only discuss the effect of the Moderate-Intensity Continuous Training (MICT) and High Intensive Interval Training (HIIT) on the signal way of AMPK- PGC-1α in skeletal muscle but not in the myocardium. The aim of this study was to compare the effects of 10 weeks HIIT and MICT on the expression of AMPK and PGC-1α in the myocardium of wistar male rats.
Methods: Wistar male rats (n=30) aged 6 weeks were randomly divided into HIIT or MICT or control (CON) group. The training groups ran on a treadmill 5 days/week for 10 weeks. HIIT group ran six times 3 minutes (0° slope) 90% of Vmax separated by 3 minutes 50% of Vmax and MICT group ran for 50min (0° slope) at 60–70% of maximal speed (Vmax). The expression of AMPK and PGC-1α were assessed by Western Blotting.
Results: After 10 weeks training, HIIT and MICT both increased the AMPK and PGC-1α expression compared with the CON group. Compared with the MICT group, the expression of AMPK and PGC-1α were significantly higher than the HIIT group (p<0.05). AMPK in MICT group were significant increased 1.16 times, and in HIIT group were significant increased 1.28 times to CON (P<0.05). PGC-1α level of HIIT was significant increased to 1.32 times to CON and also significant increased to 1.15 times to Group M (P<0.05); PGC-1α level of MICT was significant increased to 1.15 times to CON.
Conclusion：HIIT seems to improve myocardial AMPK and PGC-1α more efficiently than MICT in rats after 10 weeks training.
OR-024 Changes of mitochondrial autophagy - related genes and autophagosome after skeletal muscle blunt trauma
Authors: Dong Guijun , Bi Jun , Li Kefeng Li , Dai Lulu , Zong Wenhao
Published: October 2018

Objective Objective: To study the changes of mitochondrial autophagy-related genes and autophagosome after skeletal muscle blunt trauma, to reveal the changes of mitochondrial adaptive repair process after skeletal muscle blunt trauma, and to elucidate the mechanism of blunt trauma repair process.

Methods Methods: Sixty - four male Wistar rats were randomly divided into control group and blunt trauma group三 (divided into 12h group, 2d group, 5d group, 7d group, 10d group, 15d group and 30d group) according to the time of extraction. The expression of HIF-1α, AMPKα2, BNIP3 and NIX protein in skeletal muscle hypoxia and autophagy-related factors were measured by Western-Blot. QRT-PCR was performed to analyze the expression levels of HIF-1α, AMPKα2, BNIP3 and NIX. The ultrastructure and autophagic formation at different time points were observed by transmission electron microscopy (TEM).

Results Results: The expression of HIF-1α and AMPKα2 protein reached the peak at 12h and 2d, and the expression of HIF-1α was significantly higher than that of the control group (P <0.05). The expression of AMPKα2 was significantly higher at 5 days after injury (P <0.05), and reached the normal level at 10 days. BNIP3 began to decline after 5 days, but still higher than normal at 10 days after treatment. NIX expression peak appeared at 12h and 2d after injury, with high-express to 7d. The expression of HIF-1α and AMPKα2 mRNA was significantly higher than that of the control group (P <0.01), but decreased until 5d (P <0.05), then decreased to normal level. The mRNA expression of BNIP3 and NIX was basically the same as their protein performance. A number of autophagosomes were observed at 12 h after injury, and the number of autophagosomes increased gradually at 2-7 d. After 10 days, the number of autophagosomes decreased compared with that of 12 h-7 d after blunt. And after 15 days, the number of autophagosites decreased gradually.

Conclusions Conclusion: The changes of early stage metabolic regulator AMPKα2 and hypoxia-sensitive factor HIF-1αafter skeletal muscle blunt trauma indicated that  an energy crisis occurred in the skeletal muscle after injury, and the hypoxic environment was formed. The mitochondrial autophagy, the expression of BNIP3 and NIX showed that mitochondrial autophagy was activated and hypoxia induced mitochondrial autophagy at early skeletal muscle contusion peroid. Hypoxia-induced mitochondrial autophagy could remove the damaged mitochondria, maintain mitochondrial quality and provide raw materials for new mitochondria generation, facilitate the rapid recovery of damaged skeletal muscle, which may be a compensatory mechanism of the body response to injury.
OR-047 Mechanical stretch activates glycometabolism-related enzyme through estrogen in C2C12 myoblasts
Authors: Feng Yu , Shi RengFei , Lv JianQiang
Published: October 2018

Objective Exercise is involved with some Metabolic diseases, moderate exercise may improve glycometabolism and type 2 diabetes mellitus in menopausal female. Previous study showed that exercise increased the level of muscular estrogen in Ovariectomized rats, improved muscle mass and glycometabolism, it provided a reference to relief type 2 diabetes mellitus symptom. Until now, the effect of estrogen induced by exercise on muscular glycometabolism is not clear, the present study was designed to explore the effect of estrogen induced by mechanical stretch on glycometabolism in mouse C2C12 myoblasts.

Methods The mouse C2C12 myoblasts in vitro were plated at BioFlex Culture Plate, and assigned randomly to the control group(C), stretch group(S), SA group. SA group was cultured in growth medium with 400μg/ml anastrozole(aromatase inhibitor), other groups were cultured in GM with DMSO for 36h, and then S, SA groups were stretched by Flexcell FX-5000TM system (magnitude 15%, frequency 1Hz, duration 6hours), Cellular proteins were extracted after 24h of stretch, ELISA assay was used to detect estradiol levels, we detected the expression of HK, PI3K, the ratio of p-AKT and AKT, GLUT4 protein level by Western blotting.

Results Compared with the control group, a higher estradiol level was detected in stretch group(P<0.05), and the protein expression of HK, PI3K, the ratio of p-AKT and AKT, GLUT4 is higher(P<0.05) after stretching. The estradiol level and protein expression is lower in SAF group as compare to the stretch group(P<0.05). while there was no significant difference in estradiol level and protein expression between SF group and SAF group(P>0.05).

Conclusions Estrogen induced by mechanical stretch can improve glycometabolism-related enzyme and protein expression of mouse C2C12 myoblasts.
PO-046 Gene expression profiling of peripheral blood mononuclear cells in young male trampoline athletes
Authors: Gao Li , Yang Yong Jie , Li En Qi , Mao Jia Ning
Published: October 2018

Objective Evidence indicates that physical activity influence bone health. However, the molecular mechanisms mediating the beneficial adaptations to exercise are not well understood. The purpose of this study was to examine the differentially expressed genes in PBMC between athletes and healthy controls, and to analyze the important functional genes and signal pathways that cause increased bone mineral density in athletes, in order to further reveal the molecular mechanisms of exercise promoting bone health.

Methods Five professional trampoline athletes and five age-matched untrained college students participated in this study. Used the human expression Microarray V4.0 expression profiling chip to detect differentially expressed genes in the two groups, and performed KEGG Pathway analysis and application of STRING database to construct protein interaction Network; Real-Time PCR technology was used to verify the expression of some differential genes. 

Results Compared with healthy controls, there were significant improvement in lumbar spine bone mineral density, and 236 up-regulated as well as 265 down-regulated in serum samples of athletes. The differentially expressed genes involved 28 signal pathways, such as cell adhesion molecules. Protein interaction network showed that MYC was at the core node position. Real-time PCR results showed that the expression levels of CD40 and ITGα6 genes in the athletes were up-regulated compared with the healthy controls, the detection results were consistent with that of the gene chip.

Conclusions The findings highlight that long-term high-intensity trampoline training could induce transcriptional changes in PBMC of the athletes. These data suggest that gene expression fingerprints can serve as a powerful research tool to design novel strategies for monitoring exercise. The findings of the study also provide support for the notion that PBMC could be used as a substitute to study exercise training that affects bone health.
PO-079 Effect of Aerobic Exercise on the expression of VEGF in hippocampus and the Spatial Learning and Memorizing Abilities in CUMS-induced depressive Rats
Authors: Jin Yu , Yuan Qiongjia
Published: October 2018

Objective Through the 4 weeks aerobic exercise intervention in rats with CUMS (chronic unpredictable mild stress) to build depression model of rats, and to explore the effects of aerobic exercise intervention on hippocampal VEGF expression and spatial learning and memorizing ability in depressive model rats.

Methods 30 adult 3-month-old Sprague-Dawley rats weighing 300 ± 20 g were used. After adaptive feeding for 1 week, they were randomly divided into 3 groups: control group (C), the model group (M), and exercise group (E). M and E groups were subjected to CUMS stimulation for 4 weeks and/or aerobic exercise for 4 weeks according to different modeling procedures. After exercise or CUMS, the behavioral index was tested by sucrose preference test (SPT) and Morris water maze (MWZ). The 5-HT expression of whole brain was detected by ELISA. And Real-time PCR, Western Blotting, HE staining and immunofluorescence method test the expression of VEGF and morphological structure to change in hippocampus.

Results Compared with the C group, the sucrose intake and the percentage of syrup preference were significantly decreased in the CUMS-induced depression rats, the 5-HT depression in the whole brain was significantly decreased, the hippocampal neurons were disorderly arranged and the number was less, and the hippocampal VEGF gene and protein were significantly decreased. While aerobic exercise can significantly improve the depression-like behavior, learning and memorizing ability of rats with depression, increase the expression of 5-HT in whole brain, and the hippocampal neurons are arranged neatly, clearly and in large quantities, and increase the gene and protein expression of VEGF in hippocampus.

Conclusions Four weeks aerobic exercise intervention can significantly up-regulate the expression of VEGF in hippocampus and improve learning and memorizing ability and depressive symptoms. It was suggested that the increased expression of VEGF in the hippocampus may be one of the neurobiological mechanisms in depression and spatial learning and memorizing ability.
PO-083 Tracking Research of Body Function Monitoring on Mountain Cyclists during Plain-Plateau-Lower Plateau Training
Authors: Guo Ziyuan
Published: October 2018

Objective To explore the functional changes of mountain bike athletes in the course of continuous training of six weeks plain, plateau and lower plateau, and to provide reference basis for coaches to choose plateau training, arrange plateau training and downhill time in a targeted way.

Methods The continuous tracking and monitoring of the 6 weeks plain, 6 weeks plateau and 6 weeks lower plateau training of the 8 Anhui mountain cycling team athletes were carried out, and some functional indicators(WBC, RBC, HCT, HB, BUN, CK, T, C, etc.) were analyzed.

Results (1) Six weeks of plain training, serum BUN, CK showed a significant increase in the 5th and 6th weeks(P<0.01), serum T reached the lowest level in the 3rd week(P<0.05), and reached a higher value in the 5th and 6th weeks. (2) Six weeks of plateau training, HB and HCT have been maintained at a high value, BUN has an upward trend, CK and C have a downward trend, and T has a gradual upward trend, with no significant differences(P>0.05). (3) Six weeks of training in the lower plateau, HB and HCT began to show a gradual downward trend from the first week, starting from the fourth week, BUN has a downward trend, CK has increased significantly, serum T has decreased, and the fourth week reaches The lowest value, the fifth week began to rebound.

Conclusions (1)Plain training can gradually improve the athlete's ability to adapt to the training load and the level of function; (2)Plain training is easier to increase load strength than plateau training, and plateau training is easier to increase load than plain training. (3)Plain training and plateau training can all improve the athletes 'HB, HCT, and T levels, but plateau training is more advanced than plain training; (4)HB, HCT, and T can be maintained at a higher level within 1 week of the Lower Plateau. From the second week, HB, HCT, and T show a gradual downward trend. In the third week, HB drops to the lowest value, and in the fourth week, T reaches the lowest value.

Proposal: (1)mountain bike athletes can consider arranging 5-6 weeks of plain training before going to the plateau; (2)During plateau training, it is easy to cause BUN to rise. Coaches must plan the active recovery time of athletes; (3)Plateau training can improve the athletes 'functional level, but the time to participate in the competition in the lower plateau must be controlled within one week.
PO-002 Relationship between Air Pollution and College Students' Stamina
Authors: Chen Hongmiao , Feng Jiacheng
Published: October 2018

Objective To find out whether the air quality in the long growing areas of adolescents affects their cardiorespiratory function through experiments.

Methods Through the classified statistics of the habitation of the students at H university，and the related statistics of the physical fitness test scores in the first and last year of college (men's 1000 meters run, the women's 800-meter run and vital capacity) and the average index of air pollution.

Results 1.There are positive correlation between pollution index of key cities all over the country and vital capacity of freshman in the region, but after four years living under a common environment, correlation of female lung capacity level and regional air pollution index disappears, while the correlation enhances of boys. 2. Senior students’ vital capacity results are improved; 3.There is no obvious relation between the endurance performance of freshman and senior and regional pollution index. 

Conclusions 1 Air pollution and lung capacity are positively correlated, more exercise can weaken the effect of atmospheric pollution on lung. 2 After four years of urban life where the atmospheric pollution index is low, lung function can get some improvement. 3 Superimposed effects of smoking and air pollution is more severe to the lungs, last longer and less reversible .
PO-003 Effect of C2C12 Myotubes Function after Electrical Stimulation at Different Time
Authors: Zhao Jun , Dong Heling
Published: October 2018

Objective To study the effect of different time of electrical stimulation on C2C12 myotubes function, and further explore its molecular mechanism.

Methods An electrical stimulation was given 7 days after C2C12 myotubes differentiation, of which intensity was 30ms, 3Hz, and the stimulation time was 60mins, 120mins, and 180mins, respectively. A total of four experimental groups, including Con (control group), E60 (60 mins group), E120and E180. Microscope was used to observe the muscular myotubes form; Kits were to detect MDA and ROS; Western blot was used to detect the expression of autophagy proteins and mechanism proteins, including PGC1, p-ULK, SIRT1 and SIRT3.

Results Compared with the control group, MDA, ROS, SIRT3 increased significantly in E60 (p<0.05), p-ULK and PGC1 increased significantly (p<0.01), SIRT1 decreased significantly (p<0.05). In E120, MDA、ROS、SIRT3 and PGC1 increased significantly (p<0.01), SOD decreased significantly (p<0.05). In E180, MDA and ROS increased significantly (p<0.01), SOD decreased significantly (p<0.01).

Conclusions Moderate electrical stimulation can significantly activate oxidative stress, and further promote SIRT3, PGC1 and p-ULK expression, while excessive stimulation has the opposite effects.
PO-010 Effects of kickboxing on body composition of adolescents with obesity
Authors: Chu Ziwen
Published: October 2018

Objective In order to find a scientific and effective way to lose weight, the effect of kickboxing exercise was studied by the method of experiment. Through research, it is found that kickboxing can effectively change the body shape and body composition of the human body and achieve a better weight loss effect. This study further enriches and improves the theory of exercise and fitness, which can provide scientific guidance for obese children and adolescents to lose weight and provide a good reference for obesity of other age groups and special populations. 

Methods In this paper, 20 students of 12~14 years old in the summer class of Wuhan diet training camp were selected as the subjects. Through the test of height and weight, the 20 subjects of drug induced obesity or non secondary obesity were selected by BMI as the main basis, including 8 girls and 12 boys. kickboxing training time is 8 weeks, 3 times a week, each class 60 minutes，from warming up, basically, relax three parts. In order to study the effects of kickboxing on body composition of obese people, skin fold thickness, girth and body fat were monitored and tested. 

Results After 8 weeks of kickboxing exercise, there was a significant difference in the thickness of the upper arm skin fold of the boys P < 0.05, and the thickness of the skin fold of the lower scapula was P < 0.01, but the changes in the abdomen were not obvious. While the skin thickness of the upper arm and the abdominal skin fold were P < 0.05, and there was no significant difference in the skin fold thickness of the scapula. Male hip circumference P < 0.05 had a very significant change, chest circumference, waist circumference and waist hip ratio P > 0.05 did not change significantly. The waist circumference and waist hip ratio were significantly changed, but there was no significant change in chest circumference and hip circumference. After exercise, the changes in fat content, muscle weight and BMI were the most significant. Girls' weight, fat content, body fat percentage and BMI were the most significant changes, followed by a slight change in muscle weight.

Conclusions From the qualitative point of view, it can be concluded that the practice of kickboxing exercises can effectively develop flexibility, balance, coordination and so on. It has a significant effect on improving human posture and sports ability. From a quantitative point of view, aerobics can also reduce fat content, increase muscle weight, make the percentage of BMI, body fat change significantly, and have a significant impact on maintaining muscle content and muscle / weight ratio and laying a solid foundation for future exercise. It can be concluded that kickboxing is a suitable exercise for obese people to lose weight.
PO-025 The application of serum creatinine in training monitoring
Authors: Xu Chi
Published: October 2018

Objective Study the role of serum creatinine in physiological function assessment of weightlifters combined with other indexes, in order to evaluate the effect of phased training and arrange training plan scientifically.

Methods Taking weightlifters as the main research object, the sports features of Cr, the correlation between Cr and body composition, Cr and physical ability of the athletes were studied, and the exercise load of the athletes were monitored with other physiological and biochemical indexes. 

Results There was a significant difference in Cr value between anaerobic metabolism (ATP-CP and glycolysis) and aerobic metabolism (p < 0.01); Cr was highly correlated with lean body weight, skeletal muscle content and BMI (R2 was 0.562, 0.574, 0.871 respectively); Cr was basically consistent with the change trend of hemoglobin, testosterone, creatinine kinase and other physiological and biochemical indicators in terms of training monitoring; Cr was highly correlated with maximum anaerobic work,average anaerobic work and maximum anaerobic work/body weight (R2 was 0.625, 0.707, 0.660 respectively).

Conclusions In the sports dominated by phosphoric acid, Cr can be used to assess athletes muscle mass and non-invasive measuring level of creatine phosphate in the body. The change of Cr concentration in human body is closely related to athletic train.It can be used to control the quantity and intensity of exercise more scientifically combined with other indexes. At the same time,it can monitor the function of the kidneys in the training process. When the Cr is abnormally higher than the personal threshold,it is necessary to pay attention to the physical function of the athletes, such as diet, nutrient, weight loss control etc. Moreover,excessive fatigue should be adjusted in time to avoid overincrease of Cr which may cause kidney damage.
PO-126 Inhibition of HIF-1α Alleviates Exaggerated Pressor Response Induced by Static Muscle Contraction in Rats with Peripheral Arterial Disease
Authors: Li Jianhua , Xing Jihong
Published: October 2018

Objective Hypoxia-inducible factor 1α (HIF-1α) is a transcription factor mediating adaptive responses to hypoxia and ischemia. A prior work showed that HIF-1α is increased in sensory nerves of rats with femoral artery occlusion. The present study was to examine if the reflex response of blood pressure induced by muscle contraction was altered after injection of HIF-1α inhibitor BAY 87-2243 (BAY87) into the arterial blood supply of the ischemic hindlimb muscles. 

Methods A rat model of femoral artery ligation was used to study peripheral artery disease. Western blot analysis was employed to examine the protein levels of HIF-1α in the dorsal root ganglion (DRG) tissues. The exercise pressor reflex was evoked by static muscle contraction. 

Results HIF-1α was increased in the DRG of occluded limbs (optical density: 0.89±0.13 in control vs. 1.5±0.05 in occlusion; P < 0.05, n=6 in each group). Arterial injection of BAY87 (0.2 mg/kg) inhibited expression of HIF-1α in the DRG of occluded limbs three hours following its injection (optical density: 1.02±0.09 in occluded limbs with BAY87 vs. 1.06±0.1 in control limbs; P > 0.05, n=5 in each group). In addition, muscle contraction evoked a greater increase in blood pressure in occluded rats. BAY87 attenuated the enhanced pressor response in occluded rats to a greater degree than in control rats.

Conclusions Inhibition of HIF-1α alleviates exaggeration of the exercise pressor reflex in rats under ischemic circumstances of the hindlimbs induced by femoral artery occlusion.
PO-129 Effect of endurance training on liver NK cells in mice
Authors: Wang Zhe , Jiang Ning , Wang Xinghao , Li Jing
Published: October 2018

Objective NK cell (natural killer cell) is a large granular lymphocyte distinct from a group of T and B lymphocytes. At present, the research shows that NK cells can specifically identify target cells and release killing media and then play a killing effect. It is confirmed that the expression of IL-15 is closely related to the differentiation and maturation of NK cells. Furthermore, skeletal muscle is an endocrine tissue and plays a key role in regulating the whole-body metabolic health by synthesizing and releasing humoral factors called myokines, such as IL-15. Whether the IL-15 induced by exercise training can promote the maturation of NK cells remain unsolved. This study aimed to explore the effects of moderate endurance training on NK cells and relative mechanism.

Methods Twenty male C57BL/6J mice were randomly divided into 2 groups: control group (YC) and exercise group (YE). YC animals were fed normally for 12 weeks, YE animals were trained for 12 weeks on moderate intensity treadmill (12 m/min).Then the samples were isolated and RT-PCR was used to detect IL-15 and Nkg2d genes in the liver, Western blotting was used to detect the killer factor IFN-γ released by NK cells. Flow cytometry was used to detect NK1.1 cell markers in primary liver cells .

Results 1)Compared with the YC group, the expression level of IL-15 and Nkg2d gene in the liver tissue of YE mice increased significantly (P < 0.05,P < 0.01); 2) Compared with the YC group, the expression of IFN-γ protein in the liver tissue of the YE mice increased significantly (P < 0.05); 3) Compared with two group. The proportion of NK cells in liver cells of group YE increased significantly (P < 0.05).

Conclusions Moderate intensity endurance training can enhance the content and killing ability of NK cells through induced IL-15 in the liver.
PO-135 Application of Wireless Heart rate system in Men's Freestyle
Authors: Zhao Fan , Liu Yong , Zhou Yi
Published: October 2018

Objective Using heart rate wireless system to continuously track the morning pulse of male freestyle wrestlers after different training loads. The basic value of individual morning pulse and the interval of variation of individual morning pulse after class were established for the elite players.

Methods The paper is based on 6 elite male freestyle wrestlers, we continuously test morning pulse after different training load and recovery period，then fill in fatigue questionnaire.We use Spss statistical software to carry out statistical treatment of the experimental data, and analysis the descriptive, difference, correlation and reliability of data.

Results Men freestyle athletes base morning pulse at 43-47 / min. After high intensity, the morning pulse increased by about 6%-11%, and the range of change was basically consistent with the change of subjective feeling and heart rate in training class. The average (X) and standard deviation (SD) of the morning pulse in high intensity training class were different from each other. Combined with the results of training diary and fatigue questionnaire, the players appeared body and psychological fatigue and complained that they felt very tired. The X-SD~X SD interval can be used as the early morning pulse range for evaluating athletes' fatigue after high intensity class. The morning pulse and SpO2 was associated with exhaustion of emotional energy, negative evaluation of exercise, decreased sense of achievement, and no significant correlation with heart rate fatigue. However, these three dimensions were significantly related to mental fatigue, r>0.934.

Conclusions After high intensity, the morning pulse increased significantly compared with the basic value of morning pulse. Combined with questionnaire survey and training diary feedback, the subjective feeling of body appeared fatigue after high intensity class, which was also consistent with the change of morning pulse. Can be based on individual morning pulse changes to learn about the high intensity class fatigue situation.The morning pulse after high intensity can reach the fatigue interval, which indicates that the training intensity can stimulate the body greatly, and the gradual recovery of the morning pulse can be regarded as the state of whether or not there is overfatigue. If maintain oneself high level all the time, need to adjust training intensity in time. If you can gradually recover close to the basic value, the large-intensity training class can be well adapted to the body.
PO-195 Effects of different exercises on cyocardial cell cycles
Authors: Xie Jinfeng
Published: October 2018

Objective With the advent of the era of great health, people's health awareness is increasing.As an economical and environment-friendly way, exercise should be viewed as a “first line” strategy for prevention and treatment of arterial aging and a vital component of a contemporary public health approach for reducing the projected increase in population Cardiovascular diseases burden.The objective of this work was to study the effect of 8-week spin training and swim training on normal cyocardial cell cycle.

Methods A total of 30 C57BL/6 mice were randomly allocated to 8-week spin training and swim training program (intervention group) or no supervised exercise (control group). Normal cyocardial cell cycle proteins were measured by Western Blotting. All the outcomes were measured after 8 weeks. Repeated measures were analyzed using mean ±SD and T tests were used to analyze the data.

Results Compared with the control group, Western Blotting shows that CDK4 of spin training significantly increase (0.7698± 0.01130 to 1.111 ±0.02027, p<0.001) and CDK4 of swim training significantly increase (0.7698 ±0.01130 to 1.258± 0.01427, p<0.001) ,following 8 weeks of exercise, CyclinD1 of spin training increase (0.8558 ± 0.007912 to 1.499 ± 0.06303, p<0.001) and CyclinD1 of swim training significantly increase (0.8558 ± 0.007912 to 2.097 ± 0.3622, p<0.05) .

Conclusions The 8-week spin training and swim training was found to be effective in increasing cyocardial cell cycles proteins in normal mice. There was significant improvement inCDK4 and CyclinD1 in intervention group compared to control group.
PO-198 Effect of aerobic exercise on antioxidation ability of mice kidneys
Authors: He Sisi , Wang Haitao
Published: October 2018

Objective To study the total antioxidant capacity of the kidney in the Aged mice Total Antioxidant Capacity(T-AOC), Malondialdehyde (MDA) and superoxide dismutase (SOD) expression level by aerobic exercise. Objective to explore the effect of aerobic exercise on antioxidation ability of mice.

Methods 30 Male C57BL/6 strain mice were randomly divided into adult group ( group CC) in the 13 months old, in the control group (Group C), 5 months of quiet feeding, the aerobic exercise group (Group E) ran for 5 months, 10 per group, and the adult group (group CC) compared with the control group (Group C), The Analysis control Group (Group C) was compared with the aerobic exercise group (Group E). After 5 months of Treadmill running in Group E, Determination of antioxidant index of each group. The statistical method is processed by SPSS20.0 statistic software, the experimental data is expressed by the mean ± standard deviation, the two mean numbers are analyzed by independent sample t test, and the difference of (p<0.05) is statistically significant.

Results T-AOC of adult Group was (0.99±0.13) U/mg Prot, control group was (0.27±0.07) u/mg prot, aerobic exercise group (0.39±0.10) U/mg prot, adult group SOD as (46.32± 1.84) U/mg, control Group (29.71±7.64) U/mg, aerobic exercise group (39.85±5.83) U/mg, adult group MDA (8.15±0.16) nmol/mg, control Group (8.83±0.26) nmol/mg, The aerobic exercise group was (6.31±1.23) nmol/mg, the adult group was SOD/MDA (5.69±0.32), the control group was (3.38±0.9) and the aerobic exercise group was (6.55±1.08). Compared with the adult group, the control group T-AOC and SOD decreased significantly, MDA significantly increased, the difference was very significant (p<0.01), sod/mda significantly decreased, SOD/MDA difference was significant (p<0.01). Compared with the control group, the aerobic exercise group T-AOC and SOD obviously increased, MDA significantly decreased, the difference was significant (p<0.01), SOD/MDA increased significantly, the difference was very significant (p<0.01).

Conclusions To sum up, with the increase of the age of mice MDA expression is increased, the 18 months old SOD expression is lower than 13 months old, indicating that the antioxidant capacity with the increase in age,  The results of this study are consistent with the results of previous scholars. The results show that aerobic exercise has some antioxidant effect, and aerobic exercise can decrease expression of kidney tissue SOD, raise the expression of kidney tissue T-AOC, increase the expressionof kidney tissue, increase the expression  of SOD/MDA, and reduce the oxidative stress of kidney to some extent,  So as to protect the role of the kidney, aerobic exercise and kidney-related mechanisms also need our further study.