bluuebunny/crossref_metadata_2025 huggingface dataset:
Co-chaperone involvement in knob biogenesis implicates host-derived chaperones in malaria virulence
Authors: Diehl Mathias , Weber Sebastian , Cyrklaff Marek , Sanchez Cecilia P. , Beretta Carlo A. , Roling Lena , Simon Caroline S. , Guizetti Julien , Mayer Matthias P. , Przyborski Jude M.
Published: March 2021

The pathology associated with malaria infection is largely due to the ability of infected human erythrocytes to adhere to a number of receptors on endothelial cells within tissues and organs. This phenomenon is driven by the export of parasite-encoded proteins to the host cell, the exact function of many of which is still unknown. Here we inactivate the function of one of these exported proteins, PFA66, a member of the J-domain protein family. Although parasites lacking this protein were still able to grow in cell culture, we observed severe defects in normal host cell modification, including aberrant morphology of surface knobs, disrupted presentation of the cytoadherence molecule PfEMP1, and a total lack of cytoadherence, despite the presence of the knob associated protein KAHRP. Complementation assays demonstrate that an intact J-domain is required for recovery to a wild-type phenotype and suggest that PFA66 functions in concert with a HSP70 to carry out host cell modification. Strikingly, this HSP70 is likely to be of host origin.

Taken together, our data reveal a role for PFA66 in host cell modification, implicate human HSP70 as also being essential in this process, and uncover a KAHRP-independent mechanism for correct knob biogenesis. Our observations open up exciting new avenues for the development of new anti-malarials.
Biofoundry-assisted expression and characterisation of plant proteins
Authors: Dudley Quentin M. , Cai Yao-Min , Kallam Kalyani , Debreyne Hubert , Lopez Jose A. Carrasco , Patron Nicola J.
Published: March 2021

Many goals in synthetic biology, including the elucidation and refactoring of biosynthetic pathways and the engineering of regulatory circuits and networks, require knowledge of protein function. In plants, the prevalence of large gene families means it can be particularly challenging to link specific functions to individual proteins. However, protein characterisation has remained a technical bottleneck, often requiring significant effort to optimise expression and purification protocols. To leverage the ability of biofoundries to accelerate design-built-test-learn cycles, we present a workflow for automated DNA assembly and cell-free expression of plant proteins that accelerates optimisation and enables rapid progression to characterisation. First, we developed a phytobrick-compatible Golden Gate DNA assembly toolbox containing plasmid acceptors for cell-free expression usingE. colior wheat germ lysates as well as a set of N- and C-terminal tag parts for detection, purification, and improved expression/folding. We next optimised automated assembly of miniaturised cell-free reactions using an acoustic liquid handling platform and then compared tag configurations to identify those that increase expression. We additionally developed a luciferase-based system for rapid quantification that requires a minimal 11 aa tag and demonstrate facile removal of tags following synthesis. Finally, we show that several functional characterisation experiments can be performed with cell-free protein synthesis reactions without the need for protein purification. Together, the combination of automated assembly of DNA parts and cell-free expression reactions should significantly increase the throughput of experiments to test and understand plant protein function and enable the direct reuse of DNA parts in downstream plant engineering workflows.
In silicological modelling to uncover cooperative interactions in cancer
Authors: Selvaggio Gianluca , Chaouiya Claudine , Janody Florence
Published: March 2021

The multistep development of cancer involves the cooperation between multiple molecular lesions, as well as complex interactions between cancer cells and the surrounding tumour microenvironment. The search for these synergistic interactions using experimental models made tremendous contributions to our understanding of oncogenesis. Yet, these approaches remain labour intensive and challenging. To tackle such a hurdle, an integrative, multidisciplinary effort is required. In this article, we highlight the use of logical computational models combined to experimental validations as an effective approach to identify cooperative mechanisms and therapeutic strategies in the context of cancer biology.In silicomodels overcome limitations of reductionist approaches by capturing tumour complexity, and by generating powerful testable hypotheses. We review representative examples of logical models reported in the literature and their validation. We then provide further analyses of our logical model of Epithelium to Mesenchymal Transition (EMT), searching for additional cooperative interactions involving inputs from the tumour microenvironment and gain of function mutations in NOTCH.
Facilitated Dissociation of Nucleoid Associated Proteins from DNA in the Bacterial Confinement
Authors: Koşar Zafer , Attar A. Göktuĝ , Erbaş Aykut
Published: March 2021

Transcription machinery depends on the temporal formation of protein-DNA complexes. Recent experiments demonstrated that lifetime of the complex can also affect transcription. In parallel,in vitrosingle-molecule studies showed that nucleoid-associated proteins (NAPs) leave the DNA rapidly as the bulk concentration of the protein increases via facilitated dissociation (FD). Never-theless, whether such concentration-dependent mechanism is functional in a bacterial cell, in which NAP levels and the 3D chromosomal structure are often coupled, is not cleara priori. Here, by using extensive coarse-grained molecular simulations, we model the unbinding of specific and nonspecific dimeric NAPs from a high-molecular-weight circular DNA molecule in a cylindrical structure mimicking the cellular confinement of a bacterial chromosome. Our simulations show that physiologically relevant peak protein levels (tens of micromolar) lead to highly compact chromosomal structures. This compaction results in rapid off rates (shorter DNA-residence times) but only for specifically DNA-binding NAPs such as the factor for inversion stimulation (Fis). Contrarily, for nonspecific NAPs, the off rates decrease as the protein levels increase, suggesting an inverse FD pattern. The simulations with restrained chromosome models reveal that this inverse response is due to DNA-segmental fluctuations, and that chromosomal compaction is in favor of faster protein dissociation. Overall, our results indicate that cellular-concentration level of a structural DNA-binding protein can be highly intermingled with its DNA-residence time.
Intergenerational Effects of Early Life Starvation on Life-History, Consumption, and Transcriptome of a Holometabolous Insect
Authors: Paul Sarah Catherine , Singh Pragya , Dennis Alice B. , Müller Caroline
Published: March 2021

Intergenerational effects, also known as parental effects in which the offspring phenotype is influenced by the parental phenotype, can occur in response to factors that occur not only in early but also in late parental life. However, little is known about how these parental life stage-specific environments interact with each other and with the offspring environment to influence offspring phenotype, particularly in organisms that realize distinct niches across ontogeny. We examined the effects of parental larval starvation and adult reproductive environment on offspring traits under matching or mismatching offspring larval starvation conditions using the holometabolous, haplo-diploid insectAthalia rosae(turnip sawfly). We show that the parental larval starvation treatment had trait-dependent intergenerational effects on both life-history and consumption traits of offspring larvae, partly in interaction with offspring conditions and sex, while there was no significant effect of parental adult reproductive environment. In addition, while offspring larval starvation led to numerous gene- and pathway-level expression differences, parental larval starvation impacted fewer genes and only the ribosomal pathway. Our findings reveal that parental starvation evokes complex intergenerational effects on offspring life-history traits, consumption patterns as well as gene expression, although the effects are less pronounced than those of offspring starvation.
Effects of long-term high-temperature stress on reproductive growth and seed development in development inBrassica napus
Authors: Mácová Kateřina , Prabhullachandran Unnikannan , Spyroglou Ioannis , Štefková Marie , Pěnčík Aleš , Endlová Lenka , Novák Ondřej , Robert Hélène S.
Published: March 2021

Brassica napusis the second most important oilseed crop worldwide. Increasing average temperatures and extreme weather have a severe impact on rapeseed yield. We determined the response of three cultivars to different temperature regimes (21/18 °C, 28/18 °C and 34/18 °C), focusing on the plant appearance, seed yield, seed quality, seed viability, and embryo development. Our microscopic observations identified that embryo development is affected by high temperatures. We noticed an acceleration of its development, in addition to pattern defects. Reduced fertilization rate, increased abortion rate, and preharvest sprouting would be responsible for the low seed yield at the high-temperature regime. Hormone profiling indicates that reduced auxin levels in young seeds may cause the observed embryo pattern defects. Moreover, reduced seed dormancy may result from low ABA and IAA levels in mature seeds. Glucosinolates and oil composition measurements suggest reduced seed quality. These identified cues help understand seed thermomorphogenesis and pave the way to the development of thermoresilient rapeseed plants.

Highlight

Brassica napusflowering plants’ growth at high temperatures accelerates embryo development, causing a high seed abortion rate and reduced seed quality. Temperature-reduced ABA levels cause premature seed sprouting.
Spatial Epigenome Sequencing at Tissue Scale and Cellular Level
Authors: Deng Yanxiang , Zhang Di , Liu Yang , Su Graham , Enninful Archibald , Bai Zhiliang , Fan Rong
Published: March 2021

Spatial biology is emerging as a new frontier of biomedical research in development and disease, but currently limited to transcriptome and a panel of proteins. Here we present spatial epigenome profiling for three histone modifications (H3K27me3, H3K4me3, H3K27ac) via next-generation sequencing by combining in-tissue CUT&Tag chemistry and microfluidic deterministic barcoding. Spatial chromatin states in mouse embryos or olfactory bulbs revealed tissue type-specific epigenetic regulations, in concordance with ENCODE reference data, but providing spatially resolved genome-wide profiles at tissue scale. Using fluorescence imaging to identify the tissue pixels (20μm) each containing one nucleus allowed us to extract single-cell epigenomes in situ. Spatial chromatin state profiling in tissue may enable unprecedented opportunities to study epigenetic regulation, cell function and fate decision in normal physiology and pathogenesis.
Loss of TCF-1 regulates production of noncanonical Tregs in a prion-like manner
Authors: Harris Rebecca , Mammadli Mahinbanu , Karimi Mobin
Published: March 2021

Regulatory T cells (Tregs) are suppressive immune cells used for a variety of clinical and therapeutic applications. Canonical Tregs express CD4, FOXP3, and CD25, which are considered definitive markers of Treg status when used together. However, a subset of noncanonical Tregs expressing only CD4 and FOXP3 have recently been described in some infection contexts. The transcriptional regulation of these cells is still unclear. We found that loss of TCF-1 in all T cells in mice leads to expansion of these cells in multiple tissues in a cell-intrinsic fashion. This effect was not due to aberrant expression of FOXP3, as other functional Treg markers were also expressed. In addition, presence of TCF-1-deficient cells in a chimeric mouse induced increased production of noncanonical Tregs from WT donor cells. Therefore, targeting of TCF-1 may remove suppression on this Treg lineage, increasing the yield of these cells for use in the clinic.

Summary sentence

Loss of TCF-1 causes expansion of CD25- FOXP3+ noncanonical Tregs, and TCF-1-deficient T cells induce increased production of CD25- Tregs from WT cells.
DNA methylation aging and transcriptomic studies in horses
Authors: Horvath Steve , Haghani Amin , Peng Sichong , Hales Erin N. , Zoller Joseph A. , Raj Ken , Larison Brenda , Petersen Jessica L. , Bellone Rebecca R. , Finno Carrie J.
Published: March 2021

Human DNA methylation profiles have been used successfully to develop highly accurate biomarkers of aging (“epigenetic clocks”). Here, we describe epigenetic clocks for horses, based on methylation profiles of CpGs with flanking DNA sequences that are highly conserved between multiple mammalian species. Methylation levels of these CpGs were measured using a custom-designed Infinium array (HorvathMammalMethylChip40). We generated 336 DNA methylation profiles from 42 different horse tissues and body parts, which we used to develop five epigenetic clocks for horses: a multi-tissue clock, a blood clock, a liver clock and two dual-species clocks that apply to both horses and humans. Epigenetic age measured by these clocks show that while castration affects the basal methylation levels of individual cytosines, it does not exert a significant impact on the epigenetic aging rate of the horse. We observed that most age-related CpGs are adjacent to developmental genes. Consistently, these CpGs reside in bivalent chromatin domains and polycomb repressive targets, which are elements that control expression of developmental genes. The availability of an RNA expression atlas of these tissues allowed us to correlate CpG methylation, their corresponding contextual chromatin features and gene expression. This analysis revealed that while increased methylation of CpGs in enhancers is likely to repress gene expression, methylation of CpGs in bivalent chromatin domains on the other hand is likely to stimulate expression of the corresponding downstream loci, which are often developmental genes. This supports the notion that aging may be accompanied by increased expression of developmental genes. It is expected that the epigenetic clocks will be useful for identifying and validating anti-aging interventions for horses.
Evaluation of critical data processing steps for reliable prediction of gene co-expression from large collections of RNA-seq data
Authors: Vandenbon Alexis
Published: March 2021

Motivation

Gene co-expression analysis is an attractive tool for leveraging enormous amounts of public RNA-seq datasets for the prediction of gene functions and regulatory mechanisms. However, the optimal data processing steps for the accurate prediction of gene co-expression from such large datasets remain unclear. Especially the importance of batch effect correction is understudied.

Results

We processed RNA-seq data of 68 human and 76 mouse cell types and tissues using 50 different workflows into 7,200 genome-wide gene co-expression networks. We then conducted a systematic analysis of the factors that result in high-quality co-expression predictions, focusing on normalization, batch effect correction, and measure of correlation. We confirmed the key importance of high sample counts for high-quality predictions. However, choosing a suitable normalization approach and applying batch effect correction can further improve the quality of co-expression estimates, equivalent to a >80% and >40% increase in samples. In larger datasets, batch effect removal was equivalent to a more than doubling of the sample size. Finally, Pearson correlation appears more suitable than Spearman correlation, except for smaller datasets.

Conclusion

A key point for accurate prediction of gene co-expression is the collection of many samples. However, paying attention to data normalization, batch effects, and the measure of correlation can significantly improve the quality of co-expression estimates.
Delayed Start of the Respiratory Syncytial Virus Epidemic at the End of the 20/21 Northern Hemisphere Winter Season, Lyon, France
Authors: Casalegno Jean-sebastien , Javouhey Etienne , Ploin Dominique , Valette Martine , Fanget Remi , Targe Sandrine Couray , Myar-Dury Anne-Florence , Doret-Dion Muriel , Massoud Mona , Vanhems Phillipe , Claris Olivier , Butin Marine , Ader Florence , Bin Sylvie , Gaymard Alexandre , Lina Bruno , Morfin Florence ,   VRS study group in Lyon, Gillet Yves
Published: March 2021

The implementation of Non Pharmaceutical Interventions (NPI), triggered by the emergence of covid-19, decrease the RSV circulation. Data, from our ongoing surveillance; show a late introduction of RSV at the end of December and a 4 month delayed epidemic start without significant change in our NPI policy. This data indicates that RSV still have the potential to give a late season outbreak in northern hemisphere. RSV surveillance should be reinforced and RSV Pharmaceutical Interventions maintained for at risk neonate
Reconstitution of Kinesin-1 Activation
Authors: Chiba Kyoko , Ori-McKenney Kassandra M. , Niwa Shinsuke , McKenney Richard J.
Published: March 2021

Autoinhibition is an important regulatory mechanism for cytoskeletal motor proteins. Kinesin-1 (kinesin hereafter), the ubiquitous plus-end directed microtubule motor, is thought to be controlled by a complicated autoinihibition mechanism, but the molecular details remain unclear. Conformational changes mediated by intramolecular interactions between the C-terminal tail and N-terminal motor domains of the kinesin heavy chain (KHC) are proposed to be one facet of motor regulation. The dimeric KHC also binds two copies of the kinesin light chains (KLCs), which have been implicated in both autoinhibition and cargo-dependent activation of the tetrameric motor complex, although the precise mechanisms remain opaque. Using in vitro reconstitution, we show that the KLC strongly inhibits the kinesin-microtubule interaction via an independent mechanism from the tail-motor interaction within KHC. Kinesin cargo-adaptor proteins that bind KLC activated processive movement of the kinesin tetramer but the landing rate of these activated complexes remained low. The addition of MAP7, which specifically binds to the KHC, strongly enhanced activated motor motility by dramatically increasing the landing rate and processivity of the activated kinesin motors. Our results support a model whereby the activity of the kinesin tetramer is regulated by independent tail- and KLC-based inhibition mechanisms, and that cargo-adaptor binding to the KLC directly releases both of these inhibitions. However, we find that a third component, a non-motor MAP is required for robust activity of the activated motor. Thus, human kinesin activity is regulated by a two-factor mechanism comprised of intramolecular allosteric regulation, as well as intermolecular kinesin-adaptor and kinesin-MAP interactions.
Gene-Gene Interaction Detection with Deep Learning
Authors: Cui Tianyu , El Mekkaoui Khaoula , Reinvall Jaakko , Havulinna Aki S. , Marttinen Pekka , Kaski Samuel
Published: March 2021

We do not know the extent to which genetic interactions affect the observed phenotype in diseases, because the current interaction detection approaches are limited: they only consider interactions between the top SNPs of each gene, and only simple forms of interaction. We introduce methods for increasing the statistical power of interaction detection by taking into account all SNPs andcomplex interactionsbetween them, beyond only the currently considered multiplicative relationships. In brief, the relation between SNPs and a phenotype is captured by a gene interaction neural network (NN), and the interactions are quantified by the Shapley score between hidden nodes, which are gene representations that optimally combine information from all SNPs in the gene. Additionally, we design a new permutation procedure tailored for NNs to assess the significance of interactions. The new approach outperformed existing alternatives on simulated datasets, and in a cholesterol study on the UK Biobank it detected six interactions which replicated on an independent FINRISK dataset, four of them novel findings.
TP53loss initiates chromosomal instability in high-grade serous ovarian cancer
Authors: Bronder Daniel , Wangsa Darawalee , Zong Dali , Meyer Thomas J. , Wardenaar René , Minshall Paul , Tighe Anthony , Hirsch Daniela , Heselmeyer-Haddad Kerstin , Nelson Louisa , Spierings Diana , McGrail Joanne C. , Cam Maggie , Nussenzweig André , Foijer Floris , Ried Thomas , Taylor Stephen S.
Published: March 2021

High-grade serous ovarian cancer (HGSOC) originates in the fallopian tube epithelium and is characterized by ubiquitousTP53mutation and extensive chromosomal instability (CIN). While the direct causes of CIN are errors during DNA replication and/or chromosome segregation, mutations in genes encoding DNA replication and mitotic factors are rare in HGSOC. Thus, the drivers of CIN remain undefined. We therefore asked whether the oncogenic lesions that are frequently observed in HGSOC are capable of driving CIN via indirect mechanisms. To address this question, we genetically manipulated non-transformedhTERT-immortalized human fallopian tube epithelial cells to model homologous recombination deficiency (HRD) and oncogenic signalling in HGSOC. Using CRISPR/Cas9-mediated gene editing, we sequentially mutagenized the tumour suppressorsTP53andBRCA1, followed by overexpression of theMYConcogene. Single-cell shallow-depth whole-genome sequencing revealed that loss of p53 function was sufficient to lead to the emergence of heterogenous karyotypes harbouring whole chromosome and chromosome arm aneuploidies, a phenomenon exacerbated by subsequent loss of BRCA1 function. In addition, whole-genome doubling events were observed in independent p53/BRCA1-deficient subclones. Global transcriptomics showed thatTP53mutation was also sufficient to deregulate gene expression modules involved in cell cycle commitment, DNA replication, G2/M checkpoint control and mitotic spindle function, suggesting that p53-deficiency induces cell cycle distortions that could precipitate CIN. Again, loss of BRCA1 function and MYC overexpression exacerbated these patterns of transcriptional deregulation. Thus, our observations support a model whereby the initial loss of the key tumour suppressorTP53is sufficient to deregulate gene expression networks governing multiple cell cycle controls, and that this in turn is sufficient to drive CIN in pre-malignant fallopian tube epithelial cells.

SUMMARY STATEMENT

High-grade serous ovarian cancer is defined byTP53mutation and chromosomal instability, the cause of which remains poorly understood. We developed a novel model system that implicates cell cycle deregulation upon p53-loss as cause of CIN.
Markov State Models of Proton- and Gate-Dependent Activation in a Pentameric Ligand-Gated Ion Channel
Authors: Bergh Cathrine , Heusser Stephanie A. , Howard Rebecca J. , Lindahl Erik
Published: March 2021

Ligand-gated ion channels conduct currents in response to chemical stimuli, mediating electrochemical signaling in neurons and other excitable cells. For many channels the mechanistic details of gating remain unclear, partly due to limited structural data and simulation timescales. Here, we used enhanced sampling to simulate the pH-gated channel GLIC, and construct Markov state models (MSMs) of gating transitions. Consistent with new functional recordings reported here in oocytes, our analysis revealed differential effects of protonation and mutation on free-energy wells. Clustering of closed-versus open-like states enabled estimation of open probabilities and transition rates in each condition, while higher-order clustering affirmed conformational trends in gating. Furthermore, our models uncovered state- and protonation-dependent symmetrization among subunits. This demonstrates the applicability of MSMs to map energetic and conformational transitions between ion-channel functional states, and how they correctly reproduce shifts upon activation or mutation, with implications for modeling neuronal function and developing state-selective drugs.
Examination of the contribution of Nav1.7 to axonal propagation in nociceptors
Authors: Goodwin George , McMurray Sheridan , Stevens Edward B , Denk Franziska , McMahon Stephen B
Published: March 2021

Nav1.7 is a promising drug target for the treatment of pain because individuals with Nav1.7 loss-of-function mutations are insensitive to pain and do not have other serious neurological deficits. However, current peripherally restricted Nav1.7 inhibitors have not performed well in clinical pain trials, which may reflect a lack of understanding of the function of Nav1.7 in the transmission of nociceptive information. Although numerous studies have reported that Nav1.7 has a moderate role in peripheral transduction, the precise contribution of Nav1.7 to axonal propagation in nociceptors is not clearly defined, particularly for afferents innervating deep structures.

In this study, we examined the contribution of Nav1.7 to axonal propagation in nociceptors utilising sodium channel blockers inin vivoelectrophysiological and calcium imaging recordings from L4 in the mouse. Using the sodium channel blocker TTX (1-10μM) to inhibit Nav1.7 and other TTX-S sodium channels along the sciatic nerve, we first showed that around 2/3rdsof nociceptive neurons innervating the skin, but a lower proportion innervating the muscle (45%), are blocked by TTX. In contrast, nearly all large-sized A-fibre cutaneous afferents (95-100%) were blocked by axonal TTX. Characterisation of TTX resistant cutaneous nociceptors revealed that many were polymodal (57%) and capsaicin sensitive (57%).

Next, we examined the role of Nav1.7 in axonal propagation in nociceptive neurons by applying the selective channel blocker PF-05198007 (300nM-1μM) to the sciatic nerve between stimulating and recording sites. 100-300nM PF-05198007 blocked propagation in 63% of C-fibre sensory neurons, whereas similar concentrations did not affect propagation in rapidly conducting A-fibre neurons. We conclude that Nav1.7 has an essential contribution to axonal propagation in only around 2/3rdsof nociceptive C-fibre neurons, and a lower proportion (≤45%) of nociceptive neurons innervating muscle.
Systemic application of the TRPV4 antagonist GSK2193874 induces tail vasodilation in a mouse model of thermoregulation
Authors: O’Brien Fiona , Staunton Caroline A , Barrett-Jolley Richard
Published: March 2021

In humans the skin is a primary thermoregulatory organ, with vasodilation leading to rapid body cooling, whereas in Rodentia the tail performs an analogous function. Many thermodetection mechanisms are likely to be involved including transient receptor potential vanilloid-type 4 (TRPV4), a widely distributed ion channel with both mechanical and thermosensitive properties. Previous studies have shown that TRPV4 can act as a vasodilator by local action in blood vessels, and in this study, we investigated whether TRPV4 activity effectsmus muscularistail vascular tone and thermoregulation. We measured tail blood flow by pressure plethysmography in lightly sedatedmus muscularis(CD1 strain) at a range of ambient temperatures, with and without intraperitoneal administration of the blood brain barrier crossing TRPV4 antagonist GSK2193874. We also measured heart rate and blood pressure with and without GSK2193874. As expected for a thermoregulatory organ, we found that tail blood flow increased with temperature. However, unexpectedly we found that the TRPV4 antagonist GSK2193874 increased tail blood flow at all temperatures, and we observed changes in heart rate variability. Since TRPV4 activation stimulates the relaxation of peripheral resistance arteries (vasodilation) that would increase tail blood flow, these data suggest that increases in tail blood flow resulting from the TRPV4 antagonist may arise from a site other than the blood vessels themselves, perhaps in central cardiovascular control centres such as the hypothalamus.
Green information quality and green brand evaluation: the moderating effects of eco-label credibility and consumer knowledge
Authors: Kumar Prashant , Polonsky Michael , Dwivedi Yogesh K. , Kar Arpan
Published: March 2021

Purpose

This study aims to examine the effects of three green information quality dimensions – persuasiveness, completeness and credibility – on green brand evaluation and whether this is mediated by green brand credibility. It also examines the moderating effects of eco-label credibility and consumer knowledge on green information quality dimensions and green brand credibility relationships.

Design/methodology/approach

Using a structured questionnaire on environmentally-friendly electrical goods/electronics, cosmetic and apparel product advertisements, involving an elaboration task, this study collected usable data from 1,282 Indian consumers across 50 cities. It also undertook an assessment for three different product groups using structural equation modelling to examine proposed hypotheses and assessed moderated mediation using the Hays process model.

Findings

The study indicates that: green brand credibility mediates the effects of green information quality dimensions on green brand evaluation; consumer knowledge moderates the effects of persuasiveness and completeness on green brand credibility and eco-label credibility moderates the effects of persuasiveness and credibility on green brand credibility.

Research limitations/implications

In green information processing, this study supports the relevance of the elaboration likelihood model and the mediation effect of green brand credibility. It also presents evidence that credible eco-labels enhance green information processing. While the results are broadly consistent across the three product categories, the results may only generalizable to the environmentally-aware urban populations.

Practical implications

Help brand managers to design advertisements that add brand credibility in environmentally-aware urban markets.

Originality/value

It helps to define green information quality and the interacting effects of eco-label credibility and consumer knowledge in green information processing.
Two-phase model for mixed convection and flow enhancement of a nanofluid in an inclined channel patterned with heated slip stripes
Authors: Dutta Subhasree , Bhattacharyya Somnath , Pop Ioan
Published: March 2021

Purpose

The purpose of this study is to analyze the heat transfer and flow enhancement of an Al2O3-water nanofluid filling an inclined channel whose lower wall is embedded with periodically placed discrete hydrophobic heat sources. Formation of a thin depletion layer of low viscosity over each hydrophobic heated patch leads to the velocity slip and temperature jump condition at the interface of the hydrophobic patch.

Design/methodology/approach

The mixed convection of the nanofluid is analysed based on the two-phase non-homogeneous model. The governing equations are solved numerically through a control volume approach. A periodic boundary condition is adopted along the longitudinal direction of the modulated channel. A velocity slip and temperature jump condition are imposed along with the hydrophobic heated stripes. The paper has validated the present non-homogeneous model with existing experimental and numerical results for particular cases. The impact of temperature jump condition and slip velocity on the flow and thermal field of the nanofluid in mixed convection is analysed for a wide range of governing parameters, namely, Reynolds number (50 ≤ Re ≤ 150), Grashof number (

103≤Gr≤5×104), nanoparticle bulk volume fraction (

0.01≤φb≤0.05), nanoparticle diameter (

30≤dp≤60) and the angle of inclination (

−60°≤σ≤60°).

Findings

The presence of the thin depletion layer above the heated stripes reduces the heat transfer and augments the volume flow rate. Consideration of the nanofluid as a coolant enhances the rate of heat transfer, as well as the entropy generation and friction factor compared to the clear fluid. However, the rate of increment in heat transfer suppresses by a significant margin of the loss due to enhanced entropy generation and friction factor. Heat transfer performance of the channel diminishes as the channel inclination angle with the horizontal is increased. The paper has also compared the non-homogeneous model with the corresponding homogeneous model. In the non-homogeneous formulation, the nanoparticle distribution is directly affected by the slip conditions by virtue of the no-normal flux of nanoparticles on the slip planes. For this, the slip stripes augment the impact of nanoparticle volume fraction compared to the no-slip case.

Originality/value

This paper finds that the periodically arranged hydrophobic heat sources on the lower wall of the channel create a significant augmentation in the volume flow rate, which may be crucial to augment the transport process in mini- or micro-channels. This type of configuration has not been addressed in the existing literature.
Single-rating, multi-rating 360° performance management and organizational outcomes: evidence from the UAE
Authors: Behery Mohamed
Published: March 2021

Purpose

This study is an academic attempt to bridge the gap between Western theories and the under-researched non-western contexts by studying the characteristics of traditional and modern performance management systems (PMSs) in the United Arab Emirates (UAE). Drawing on the expectancy theory, this study aims to discuss the significant causal relationship between the implementation of single-rating, multi-rating 360° performance management (PM) and organizational outcomes such as trust, commitment, satisfaction and intention to leave.

Design/methodology/approach

Using the self-reported measures and survey method, data were collected from 439 employees from different organizations across the UAE. Explanatory factor analysis, simple linear regression and multi-group were used to test the proposed conceptual model and examine the mediation and moderation impact.

Findings

The study explored the best-practices attributes of the traditional single-rating, multi-rating 360° PM within a non-western context. This study also provides empirical evidence on the significant role of uncertainty avoidance and power distance orientation as a mediator between the relationship between these PMSs and trust, commitment, job satisfaction and intention to leave. Finally, this paper examined the effect of many demographic variables (such as gender, age, industry type […]) on the relationship between the independent variables and the dependent variables.

Originality/value

This study extends research on PM theories and models. Another important aspect of this study is that its model has been tested on the UAE’s data, an underrepresented geographic region in the management literature. Given all the PM’s positive characteristics, the way that this feedback is viewed and interpreted by employees may be moderated according to the employee’s management level, age, gender and many other demographics.
A novel MADM approach to the ground-handling agent selection problem in B2B markets
Authors: Bakır Mahmut , Özdemir Emircan , Akan Şahap
Published: March 2021

Purpose

Ground-handling services are important for effective aircraft operations in the air transportation system. Airlines often outsource these services to ground-handling agents through business-to-business (B2B) marketing decisions. Therefore, this paper aims to address the problem of ground-handling agent selection in the airline industry.

Design/methodology/approach

A real-world case study was carried out to demonstrate the applicability of the integrated best worst method and fuzzy multi-attribute ideal real comparative analysis (F-MAIRCA) approach to solve ground-handling agent selection problems under uncertainty and imprecision. A two-stage sensitivity analysis was also conducted to ensure the credibility and validity of the application.

Findings

In the weighting stage, “Quality” was determined as the most important criterion in terms of supplier performance. With regard to the performance of the ground-handling agents, A2 was found as the optimal supplier in terms of both credibility and validity.

Practical implications

This study enumerated several criteria that ground-handling agents must meet in order to effectively supply services for the airlines. In addition, this study provides a novel framework from which managers can gain additional benefits from their businesses. Finally, it is concluded that this approach will help airline managers quantitatively in choosing the most appropriate ground-handling agent.

Originality/value

The contributions of this study to the existing literature are twofold. First, we propose a novel multiple attribute decision-making approach to address the problem of supplier selection for airlines under uncertainty and imprecision. Second, the selection of ground-handling agents from the B2B perspective is addressed for the first time in literature.
Perceptions of undergraduate students on the effect of cooperative learning on academic achievement
Authors: Keramati Mohammad Reza , Gillies Robyn Margaret
Published: March 2021

Purpose

The purpose of this study is to examine the effect of embedding cooperative learning (CL) into the Primary and Secondary Education Course (PSEC) on the academic achievement of undergraduate university students. The study also sought to gauge the perceptions of these students application of CL.

Design/methodology/approach

This was a mixed methods sequential explanatory design involving 136 undergraduate university students who participated in a 16 week semester intervention involving the implementation of CL into their PSEC. Achievement data were collected from all students pre- and post-intervention to determine if there were significant differences between the experimental and control conditions. Forty-four participants from the experimental condition were also interviewed post-intervention on their experiences of CL.

Findings

The results showed that there was a significant difference between the academic achievement of students in the experimental and control groups in favor of students in the experimental group (p < 0.001). The perceptions of participants in the experimental condition indicated that CL not only created an empathetic, safe and pleasant learning environment and strengthened students' individual and communicative skills, but it also helped to develop an understanding of quality learning.

Research limitations/implications

The results of this study have the potential to influence university instructors by demonstrating how CL provides opportunities to not only improve student learning but also their attitude to learning.

Originality/value

This study, while demonstrating the positive effect of CL on students' academic achievement also revealed the potential this approach to teaching and learning has for embedding it in other university courses.
Is it “just” planning? Exploring the integration of social justice education in an elementary language arts methods course thematic unit
Authors: Allee-Herndon Karyn A. , Kaczmarczyk Annemarie B. , Buchanan Rebecca
Published: March 2021

Purpose

The purpose of this paper is to examine undergraduate elementary education teacher candidates’ abilities to successfully integrate social justice teaching into their interdisciplinary ELA and social studies thematic units. The projects were analyzed to determine the extent to which, if any, social justice education has been addressed.

Design/methodology/approach

This study used purposive sampling of two sections of an elementary writing methods course. Students were grouped into Professional Learning Communities (PLCs) to design an integrated thematic English Language Arts (ELA), Social Studies and Social Justice unit. At the conclusion of their project, components of their units were analyzed using the Social Justice Continuum of Teacher Development.

Findings

Overall, the results indicate that candidates were likely to plan for inclusive practices in their instructional units. There was significant attention across units to inclusive materials and content, but there was very little attention to critical or transformative practices in planning. This likely indicates candidates’ awareness about the need for diverse content but tells us little about their ability to critically analyze the power structures themselves that contribute to the need for inclusive practices.

Originality/value

Before classroom teachers can be expected to engage in critical conversations in their own classrooms, the experiences they have within their preparation programs need to be considered. These findings indicate more explicit work must be done to support candidates in their ability to critically analyze hegemonic power structures and to engage their students in learning experiences that move beyond using diverse resources into teaching advocacy strategies to students.
Social network analysis and data visualization of MIS international collaboration in Taiwan
Authors: Chuang Yung-Ting , Chen Yi-Hsi
Published: March 2021

Purpose

The purpose of this paper is to apply social network analysis (SNA) to study faculty research productivity, to identify key leaders, to study publication keywords and research areas and to visualize international collaboration patterns and analyze collaboration research fields from all Management Information System (MIS) departments in Taiwan from 1982 to 2015.

Design/methodology/approach

The authors first retrieved results encompassing about 1,766 MIS professors and their publication records between 1982 and 2015 from the Ministry of Science and Technology of Taiwan (MOST) website. Next, the authors merged these publication records with the records obtained from the Web of Science, Google Scholar, IEEE Xplore, ScienceDirect, Airiti Library and Springer Link databases. The authors further applied six network centrality equations, leadership index, exponential weighted moving average (EWMA), contribution value and k-means clustering algorithms to analyze the collaboration patterns, research productivity and publication patterns. Finally, the authors applied D3.js to visualize the faculty members' international collaborations from all MIS departments in Taiwan.

Findings

The authors have first identified important scholars or leaders in the network. The authors also see that most MIS scholars in Taiwan tend to publish their papers in the journals such as Decision Support Systems and Information and Management. The authors have further figured out the significant scholars who have actively collaborated with academics in other countries. Furthermore, the authors have recognized the universities that have frequent collaboration with other international universities. The United States, China, Canada and the United Kingdom are the countries that have the highest numbers of collaborations with Taiwanese academics. Lastly, the keywords model, system and algorithm were the most common terms used in recent years.

Originality/value

This study applied SNA to visualize international research collaboration patterns and has revealed some salient characteristics of international cooperation trends and patterns, leadership networks and influences and research productivity for faculty in Information Management departments in Taiwan from 1982 to 2015. In addition, the authors have discovered the most common keywords used in recent years.
Reliability, uncertainty and the management of error: New perspectives in the COVID‐19 era
Authors: Schulman Paul R.
Published: March 2021

This essay argues for the importance of error as an organizing concept in the management of hazardous technical systems to high levels of reliability and safety. The concept of “error” has been essential to the development of high reliability organizations (HROs). As practiced in HROs, error management has also been an important strategy for the management of uncertainty. “Uncertainty” has been conceived by some analysts as a condition that can convey little or no reliable information about its own boundary conditions or its specific threat to the operation of complex systems. The argument here is that uncertainty is differentiated and specified in HROs and provides important information in relation to error. Uncertainty does not, in the special context of HROs, end the possibility and practice of reliable management. In fact, error in HROs can be a starting point for the further analysis of ways in which uncertainty itself can be managed reliably. But the argument offered here does not mean that uncertainty does not challenge reliability in other settings. The COVID‐19 pandemic is offered as an example of how uncertainties may invalidate even the application of "reliability" as a performance standard in certain domains of management and policy.
A plasmid toolset for CRISPR‐mediated genome editing and CRISPRi gene regulation in Escherichia coli
Authors: Jervis Adrian J. , Hanko Erik K.R. , Dunstan Mark S. , Robinson Christopher J. , Takano Eriko , Scrutton Nigel S.
Published: March 2021

CRISPR technologies have become standard laboratory tools for genetic manipulations across all kingdoms of life. Despite their origins in bacteria, the development of CRISPR tools for engineering bacteria has been slower than for eukaryotes; nevertheless, their function and application for genome engineering and gene regulation via CRISPR interference (CRISPRi) has been demonstrated in various bacteria, and adoption has become more widespread. Here, we provide simple plasmid‐based systems for genome editing (gene knockouts/knock‐ins, and genome integration of large DNA fragments) and CRISPRi in E. coli using a CRISPR‐Cas12a system. The described genome engineering protocols allow markerless deletion or genome integration in just seven working days with high efficiency (> 80% and 50%, respectively), and the CRISPRi protocols allow robust transcriptional repression of target genes (> 90%) with a single cloning step. The presented minimized plasmids and their associated design and experimental protocols provide efficient and effective CRISPR‐Cas12 genome editing, genome integration and CRISPRi implementation. These simple‐to‐use systems and protocols will allow the easy adoption of CRISPR technology by any laboratory.
Comparative anatomic and morphometric examination of the interosseous muscle, sesamoid ligaments and flexor tendons of the fetlock in South American camelids
Authors: Schraml Susanne , Barrios Santos William Arthur , Mülling Christoph , Bässler Claus , Hagen Jenny
Published: March 2021

This study presents a comparative morphological and morphometrical examination of the interosseous muscle (IOM), digital flexor tendons and associated structures of all four species of South American Camelids (SACs): Lama (Lama glama), alpaca (Vicugna pacos), guanaco (Lama guanicoe) and vicuna (Vicugna vicugna). Our findings show that the lamoid interosseous muscle and digital flexor tendons are very similar between species and differences, which have been reported previously, are possibly due to diverging examiner interpretation. Lumbricalis muscles were mostly found as single muscles in this study. The definition of the terminal and dorsal branches of the interosseous muscle is critically revised. The analysis of morphometric data indicates that the size of the individual has a substantial influence on the cross‐sectional area (CSA) of the examined structures. Metacarpal/metatarsal bone width is a main predictor of CSA, while the factor species only shows significance in specific sample points. For lama and alpaca, no differences in CSA in relation to species as factor were found. The IOM shows the largest CSAs of the three examined structures in all species, which implies an important load‐bearing function. Considerable individual variation remains unexplained by the mentioned factors. This lowers the prospect of CSA reaching diagnostic importance in SAC fetlock pathology.