bluuebunny/crossref_metadata_2025 huggingface dataset:
Mixed Endometrial Epithelial Carcinoma: Epidemiology, Treatment and Survival Rates—A 10-Year Retrospective Cohort Study from a Single Institution
Authors: Pappa Christina , Le Thanh Valentina , Smyth Sarah Louise , Zouridis Andreas , Kashif Ammara , Sadeghi Negin , Sattar Alisha , Damato Stephen , Abdalla Mostafa , Laganà Antonio Simone , Ferrari Federico , Kehoe Sean , Addley Susan , Soleymani majd Hooman
Published: October 2023

Mixed endometrial carcinoma (MEEC) refers to rare endometrial tumours that are composed of two or more distinct histotypes, at least one of which is serous or clear cell. The aim of this study was to evaluate the epidemiology, treatment outcomes and survival rates of patients with mixed endometrial carcinoma. The medical records of 34 patients diagnosed with MEEC between March 2010 and January 2020 were reviewed retrospectively. Clinicopathological variables and treatment strategies were assessed, and overall survival and disease-free survival rates were evaluated. The histology of endometrioid and serous component was found in 26 (76.5%) patients, followed by serous and clear-cell components (5/34, 14.5%) and mixed endometrioid serous and clear-cell components (3/34, 8.8%). The median age at diagnosis was 70 years (range 52–84), and the median follow-up time was 55 months. The 5-year disease-free survival and the 5-year overall survival were 50.4% and 52.4%, respectively. Advanced disease stage was identified as an independent predictor of inferior disease-free (<0.003) and overall survival (p < 0.001). Except for stage, none of the traditional prognostic factors was associated with disease recurrence or death from disease. MEECs represent rare high-risk endometrial carcinomas with significant diagnostic and treatment challenges. Undoubtedly, the implementation of a molecular analysis can offer further diagnostic and management insights.
Atomic Insights into the Structural Properties and Displacement Cascades in Ytterbium Titanate Pyrochlore (Yb2Ti2O7) and High-Entropy Pyrochlores
Authors: Azeem M. Mustafa , Wang Qingyu
Published: October 2023

Pyrochlore oxides (A2B2O7) are potential nuclear waste substrate materials due to their superior radiation resistance properties. We performed molecular dynamics simulations to study the structural properties and displacement cascades in ytterbium titanate pyrochlore (Yb2Ti2O7) and high-entropy alloys (HEPy), e.g., YbYTiZrO7, YbGdTiZrO7, and Yb0.5Y0.5Eu0.5Gd0.5TiZrO7. We computed lattice constants (LC) (ao) and threshold displacement energy (Ed). Furthermore, the calculation for ao and ionic radius (rionic) were performed by substituting a combination of cations at the A and B sites of the original pyrochlore structure. Our simulation results have demonstrated that the lattice constant is proportional to the ionic radius, i.e., ao α rionic. Moreover, the effect of displacement cascades of recoils of energies 1 keV, 2 keV, 5 keV, and 10 keV in different crystallographic directions ([100], [110], [111]) was studied. The number of defects is found to be proportional to the energy of incident primary knock-on atoms (PKA). Additionally, the Ed of pyrochlore exhibits anisotropy. We also observed that HEPy has a larger Ed as compared with Yb2Ti2O7. This establishes that Yb2Ti2O7 has characteristics of lower radiation damage resistance than HEPy. Our displacement cascade simulation result proposes that HEPy alloys have more tendency for trapping defects. This work will provide atomic insights into developing substrate materials for nuclear waste applications.
Sr and Nd Isotopes in Mineral Fractions of Ferromanganese Crusts from the Northernmost Pacific
Authors: Mikhailik Pavel , Vishnevskaya Irina , Yi Liang , Soloshenko Natalia , Pellinen Vadim
Published: October 2023

A study of the isotopic compositions of neodymium and strontium in four mineral fractions of the hydrogenous ferromanganese crusts of the northernmost Pacific has been carried out for the first time. The relationship of the 87Sr/86Sr ratio and εNd value of the residual fraction in heterochronous layers of the ferromanganese crusts have shown that the sources of detrital matter are from rock weathering by the Yukon River, the Kuskokwim River, the Anadyr River and the rivers of Kamchatka. The amount of aluminosilicate impurity does not affect the isotopic compositions of the chemogenic (loosely bound, Mn oxides or manganese, hydrous Fe oxides or ferrous) fractions. The decreased εNd value in the ferrous fraction seems to be related to the presence of rare earth element (REE) phosphate complexes in seawater. The increase in those complexes is a consequence of melt waters entering the ocean during warming periods. Our data indicate that the carbonate REE complexes are not the dominant form of the REEs in the northernmost Pacific, which leads to the fact that the neodymium isotopic compositions of the manganese and ferrous fractions of hydrogenous ferromanganese crusts are different. A decrease in εNd value in the ferrous fraction may be a marker of a local increase in the surface water bioproductivity. Our data also show that the increase of the REE in the ferrous fraction relative to the manganese fraction does not reflect the participation of hydrothermal matter in the formation of mixed hydrogenous–hydrothermal crusts but is a consequence of an increase in the REE phosphate complexes.
Role of Shape and Kinematics in the Hydrodynamics of a Fish-like Oscillating Hydrofoil
Authors: Gupta Siddharth , Sharma Atul , Agrawal Amit , Thompson Mark C. , Hourigan Kerry
Published: October 2023

In the present two-dimensional numerical study, we investigate the roles of geometrical parameters of a hydrofoil (shape/curvature of the leading and trailing edges and thickness) and kinematic parameters (phase difference between heave and pitch (ϕ)) on the propulsive performance of different-shaped hydrofoils oscillating at maximum angles of attack up to αmax=30∘. The study was carried out at a fixed non-dimensional maximum heave to chord ratio h∘/C=0.75, Strouhal number St=0.25, and Reynolds number Re=5000. Our findings reveal that hydrofoil performance and stability improve with leading and trailing edge curvatures but decline as thickness increases. By analyzing the near-wake structure, we establish that even minimal flow separation increases power consumption while moderate flow separation enhances thrust. Over the range of different-shaped hydrofoils at different αmax and ϕ, maximum propulsion efficiency occurs for those parameters for which there is a small degree of flow separation but with no roll-up of a separating vortex. In comparison, maximum thrust generation occurs when there is a moderately strong flow separation but without induction of a significant amount of fluid around the trailing edge. These insights offer valuable knowledge for understanding fish propulsion efficiency and have applications in designing autonomous underwater vehicles (AUVs) and micro-air vehicles (MAVs).
Investigating on the Pavement Performance of Multi-Source Solid Wastes by Cement and Fly Ash
Authors: Shan Long , Li Hongbo , Zhao Jing , Zhang Xuanshuo , Kang Xinrui , Gao Xing , Zhou Zhiyao
Published: October 2023

In order to advance the utilization rate of multi-source solid wastes in the Ningxia region of China, 16 groups of pavement base mixtures were designed with cement and fly ash (FA) as binders, steel slag (SS), silicon manganese slag (SMS), and recycled crushed stone (RCS) as composite aggregates. The evolution laws of mechanical and frost resistance properties of the mixture were investigated by unconfined compressive strength (UCS), indirect tensile strength (ITS), freeze–thaw (FT), and ultrasonic detection tests. Then, the strength formation mechanisms were revealed by microscopic characterization technology. The mathematical models between UCS-ITS, UCS-ultrasonic amplitude, FT cycles-UCS damage, and frost resistance coefficient-relative dynamic elastic modulus Er were established. The results show that cement content and curing age exhibited a positive effect on the mechanical strength and frost resistance of the mixture. When the replacement rate of SS was 60%, the mechanical strength and frost resistance were preferable. The R2 of the strength relationship models constructed was greater than 0.9, indicating high fitting accuracy. With the extension of the curing age, the cementitious products such as C-S-H (hydrated calcium silicate) and AFt (ettringite) developed entirely, and they were interlocked and cemented with each other, resulting in the micro-morphology developed from the three-dimensional network structure to the dense system. The macroscopic behavior incarnated that the mechanical strength and frost resistance of the mixture were significantly enhanced.
Evaluating the Biomechanical Integrity of Various Constructs Utilized for First Metatarsophalangeal Joint Arthrodesis: A Systematic Review
Authors: Balu Abhinav R. , Baumann Anthony N. , Tsang Terence , Talaski Grayson M. , Anastasio Albert T. , Walley Kempland C. , Adams Samuel B.
Published: October 2023

The first metatarsophalangeal (MTP) joint is a frequently loaded joint, handling loads up to 90% of bodyweight. First MTP arthrodesis is a frequently performed procedure designed to improve pain in patients with degenerative MTP joint disease. There are a wide variety of fixation constructs for this procedure without consensus on the most effective method. The purpose of this study was to compare the biomechanical integrity of various constructs utilized for first MTP arthrodesis. A systematic review of the literature was conducted in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. PubMed, CINAHL, MEDLINE, and Web of Science databases were searched from inception to 18 June 2023. Articles discussing the biomechanics of first MTP arthrodesis constructs were included. A total of 168 articles were retrieved. A total of 20 articles involving 446 cadaveric and synthetic bone constructs were included in the final review. Of the six articles comparing dorsal plating with compression screws to crossed interfragmentary screws, five found that dorsal plating had significantly higher stiffness. All three studies assessing shape-memory staples found them to be significantly less stable than crossed screws or dorsal plates alone. Both studies evaluating fully threaded screws found them to be stronger than crossed cancellous screws. Wedge resections have been shown to be 10 times stronger than standard planar or conical excision. Dorsal plating with compression screws is the gold standard for MTP arthrodesis. However, more research into newer methods such as fully threaded screws and wedge resections with an increased focus on translation to clinical outcomes is needed.
Evaluation of Various Topological Indices of Flabellum Graphs
Authors: Shi Xiaolong , Kosari Saeed , Ahmad Uzma , Hameed Saira , Akhter Sadia
Published: October 2023

Graph theory serves as an engaging arena for the investigation of proof methods within the field of discrete mathematics, and its findings find practical utility in numerous scientific domains. Chemical graph theory is a specialized branch of mathematics that uses graphs to represent and analyze the structure and properties of chemical compounds. Topological indices are mathematical properties of graphs that play a crucial role in chemistry. They provide a unique way to connect the structural characteristics of chemical compounds to their corresponding molecular graphs. The flabellum graph Fn(k,j) is obtained with the help of k≥2 duplicates of the cycle graph Cn with a common vertex (known as, central vertex). Then, in j of these duplicates, additional edges are added, joining the central vertex to all non-adjacent vertices. In this article, we compute different degree-based topological indices for flabellum graphs, including some well known indices, such as the Randić index, the atom bond connectivity index, the geometric–arithmetic index, and the Zagreb indices. This research provides an in-depth examination of these specific indices within the context of flabellum graphs. Moreover, the behavior of these indices is shown graphically, in terms of the parameters j,k, and n. Additionally, we have extended the concept of the first Zagreb index, to address the issue of cybercrime. This application enables us to identify criminals who exhibit higher levels of activity and engagement in multiple criminal activities when compared to their counterparts. Furthermore, we conducted a comprehensive comparative analysis of the first Zagreb index against the closeness centrality measure. This analysis sheds light on the effectiveness and relevance of the topological index in the context of cybercrime detection and network analysis.
Enhancing Machine Translation Quality Estimation via Fine-Grained Error Analysis and Large Language Model
Authors: Jung Dahyun , Park Chanjun , Eo Sugyeong , Lim Heuiseok
Published: October 2023

Fine-grained error span detection is a sub-task within quality estimation that aims to identify and assess the spans and severity of errors present in translated sentences. In prior quality estimation, the focus has predominantly been on evaluating translations at the sentence and word levels. However, such an approach fails to recognize the severity of specific segments within translated sentences. To the best of our knowledge, this is the first study that concentrates on enhancing models for this fine-grained error span detection task in machine translation. This study introduces a framework that sequentially performs sentence-level error detection, word-level error span extraction, and severity assessment. We present a detailed analysis for each of the methodologies we propose, substantiating the effectiveness of our system, focusing on two language pairs: English-to-German and Chinese-to-English. Our results suggest that task granularity enhances performance and that a prompt-based fine-tuning approach can offer optimal performance in the classification tasks. Furthermore, we demonstrate that employing a large language model to edit the fine-tuned model’s output constitutes a top strategy for achieving robust quality estimation performance.
Orthodontic Aligners: Current Perspectives for the Modern Orthodontic Office
Authors: Kau Chung How , Soh Jen , Christou Teti , Mangal Akanksha
Published: October 2023

Orthodontic aligners are changing the practice of orthodontics. This system of orthodontic appliances is becoming the mainstay appliance of choice for orthodontic offices in many countries. Patient preferences and lifestyle needs have made this appliance the primary choice when seeking care. In the early days, appliances lacked the efficiency and effectiveness of traditional bracket-wire systems, but modern systems are now able to handle a more comprehensive orthodontic caseload. Current systems provide newer biomechanical strategies and artificial intelligence-driven tooth movements for better outcomes. These improvements now mean that an orthodontist can be better prepared to manage a larger number of orthodontic malocclusions. This paper aims to discuss some of the evolution of orthodontic aligners and to describe to orthodontists the fundamentals of aligner therapy. In addition, it will provide an evidence-based outcome to the existing treatment outcomes in the current literature.
Facile Synthesis of Microsphere-like Co0.85Se Structures on Nickel Foam for a Highly Efficient Hydrogen Evolution Reaction
Authors: Rajesh John Anthuvan , Kim Jae-Young , Kang Soon-Hyung , Ahn Kwang-Soon
Published: October 2023

Microsphere-shaped cobalt selenide (Co0.85Se) structures were efficiently synthesized via a two-step hydrothermal process. Initially, cobalt hydroxide fluoride (Co(OH)F) microcrystals were prepared using a hydrothermal method. Subsequently, Co0.85Se microsphere-like structures were obtained through selenization. Compared to Co(OH)F, the microsphere-like Co0.85Se structure exhibited outstanding catalytic activity for the hydrogen evolution reaction (HER) in a 1.0 M KOH solution. Electrocatalytic experiments demonstrated an exceptional HER performance by the Co0.85Se microspheres, characterized by a low overpotential of 148 mV and a Tafel slope of 55.7 mV dec−1. Furthermore, the Co0.85Se electrocatalyst displayed remarkable long-term stability, maintaining its activity for over 24 h. This remarkable performance is attributed to the excellent electrical conductivity of selenides and the highly electroactive sites present in the Co0.85Se structure compared to Co(OH)F, emphasizing its promise for advanced electrocatalytic applications.
Detection of Viable but Non-Culturable (VBNC)-Campylobacter in the Environment of Broiler Farms: Innovative Insights Delivered by Propidium Monoazide (PMA)-v-qPCR Analysis
Authors: Reichelt Benjamin , Szott Vanessa , Stingl Kerstin , Roesler Uwe , Friese Anika
Published: October 2023

Campylobacteriosis cases in humans are of global concern, with high prevalence rates in the poultry reservoir considered the most important source of infection. Research findings show Campylobacters’ ability to enter a viable but non-culturable (VBNC) state, remaining “viable” but unable to grow on culture media. We explored the persistence of VBNC states in specific environments, particularly at broiler farms, as this state may lead to an underestimation of the present Campylobacter prevalence. For VBNC detection, a propidium monoazide PMA-dye viability qPCR (v-qPCR) was used in combination with cultivation methods. We examined samples collected from broiler farm barns and their surroundings, as well as chicken manure from experimental pens. In addition, the tenacity of culturable and VBNC-Campylobacter was studied in vitro in soil and water. In a total of three visits, Campylobacter was not detected either culturally or by v-qPCR (no Campylobacter DNA) in the environment of the broiler farms. In four visits, however, VBNC-Campylobacter were detected both inside and outside the barns. The overall prevalence in environmental samples was 15.9% for VBNC-Campylobacter, 62.2% for Campylobacter DNA, and 1.2% for culturable C. jejuni. In the experimental pens, no cultivable C. jejuni was detected in chicken manure after 24 h. Strikingly, “VBNC-Campylobacter” persisted even after 72 h. “VBNC-Campylobacter” were confirmed in barn surroundings and naturally contaminated chicken manure. Laboratory studies revealed that VBNC-Campylobacter can remain intact in soil for up to 28 days and in water for at least 63 days, depending on environmental conditions.
Phloem-Localized Insect-Transmitted Bacteria Associated with Plant Diseases
Authors: Bertaccini Assunta
Published: October 2023

In the last three decades, an increasing number of plant diseases associated with the presence of phloem-localized insect-transmitted bacteria have been observed around the world, causing serious economic losses [...]
Synthesis, Anticancer Activity, and In Silico Studies of 5-(3-Bromophenyl)-N-aryl-4H-1,2,4-triazol-3-amine Analogs
Authors: Ahsan Mohamed Jawed , Gautam Krishna , Ali Amena , Ali Abuzer , Altamimi Abdulmalik Saleh Alfawaz , Salahuddin  , Alossaimi Manal A. , Lakshmi S. V. V. N. S. M. , Ahsan Md. Faiyaz
Published: October 2023

In the current study, we described the synthesis of ten new 5-(3-Bromophenyl)-N-aryl-4H-1,2,4-triazol-3-amine analogs (4a–j), as well as their characterization, anticancer activity, molecular docking studies, ADME, and toxicity prediction. The title compounds (4a–j) were prepared in three steps, starting from substituted anilines in a satisfactory yield, followed by their characterization via spectroscopic techniques. The National Cancer Institute (NCI US) protocol was followed to test the compounds’ (4a–j) anticancer activity against nine panels of 58 cancer cell lines at a concentration of 10−5 M, and growth percent (GP) as well as percent growth inhibition (PGI) were calculated. Some of the compounds demonstrated significant anticancer activity against a few cancer cell lines. The CNS cancer cell line SNB-75, which showed a PGI of 41.25 percent, was discovered to be the most sensitive cancer cell line to the tested compound 4e. The mean GP of compound 4i was found to be the most promising among the series of compounds. The five cancer cell lines that were found to be the most susceptible to compound 4i were SNB-75, UO-31, CCRF-CEM, EKVX, and OVCAR-5; these five cell lines showed PGIs of 38.94, 30.14, 26.92, 26.61, and 23.12 percent, respectively, at 10−5 M. The inhibition of tubulin is one of the primary molecular targets of many anticancer agents; hence, the compounds (4a–j) were further subjected to molecular docking studies looking at the tubulin–combretastatin A-4 binding site (PDB ID: 5LYJ) of tubulin. The binding affinities were found to be efficient, ranging from −6.502 to −8.341 kcal/mol, with two major electrostatic interactions observed: H-bond and halogen bond. Ligand 4i had a binding affinity of −8.149 kcal/mol with the tubulin–combretastatin A-4 binding site and displayed a H-bond interaction with the residue Asn258. The ADME and toxicity prediction studies for each compound were carried out using SwissADME and ProTox-II software. None of the compounds’ ADME predictions showed that they violated Lipinski’s rule of five. All of the compounds were also predicted to have LD50 values between 440 and 500 mg/kg, putting them all in class IV toxicity, according to the toxicity prediction. The current discovery could potentially open up the opportunity for further developments in cancer.
Insights into the Sensing Mechanism of a Metal-Oxide Solid Solution via Operando Diffuse Reflectance Infrared Fourier Transform Spectroscopy
Authors: Spagnoli Elena , Valt Matteo , Gaiardo Andrea , Fabbri Barbara , Guidi Vincenzo
Published: October 2023

Recently, the influence of Nb addition in the oxide solid solution of Sn and Ti was investigated with regard to the morphological, structural and electrical properties for the production of chemoresistive gas sensors. (Sn,Ti,Nb)xO2-based sensors showed promising features for ethanol monitoring in commercial or industrial settings characterized by frequent variation in relative humidity. Indeed, the three-metal solid solution highlighted a higher response level vs. ethanol than the most widely used SnO2 and a remarkably low effect of relative humidity on the film resistance. Nevertheless, lack of knowledge still persists on the mechanisms of gas reaction occurring at the surface of these nanostructures. In this work, operando Diffuse Reflectance Infrared Fourier Transform spectroscopy was used on SnO2- and on (Sn,Ti,Nb)xO2-based sensors to combine the investigations on the transduction function, i.e., the read-out of the device activity, with the investigations on the receptor function, i.e., compositional characterization of the active sensing element in real time and under operating conditions. The sensors performance was explained by probing the interaction of H2O and ethanol molecules with the material surface sites. This information is fundamental for fine-tuning of material characteristics for any specific gas sensing applications.
Unraveling the Connection: Visceral Adipose Tissue and Vitamin D Levels in Obesity
Authors: Cominacini Mattia , Fumaneri Alessia , Ballerini Linda , Braggio Michele , Valenti Maria Teresa , Dalle Carbonare Luca
Published: October 2023

Vitamin D deficiency and insufficiency are widespread on a global scale, with multiple factors playing a role in their development, such as limited exposure to sunlight, inadequate dietary consumption, as well as obesity and abdominal fat accumulation. Abdominal obesity, assessed with waist circumference (WC), is associated with metabolic syndrome and has been linked to low vitamin D levels. This study aimed to investigate the relationship between visceral adipose tissue (VAT) and vitamin D levels, particularly examining the potential threshold for vitamin D storage and sequestration using adipose tissue. The study was conducted between 2020 and 2022 with 58 patients from an internal medicine outpatient department. Patients with certain medical conditions and those taking medications affecting bone metabolism were excluded. Blood samples were collected at baseline and after 6 months of monthly cholecalciferol supplementation. Ultrasonography was used to evaluate adipose tissue measurements, including subcutaneous adipose tissue thickness, VAT, preperitoneal adipose tissue (PPAT), and prerenal adipose tissue (PRAT). Anthropometric measures such as the waist-to-hip ratio and waist-to-height ratio were also assessed. The results showed that all subjects had significant hypovitaminosis D at baseline. After 6 months of supplementation, the mean increase in vitamin D levels was 9.6 ng/mL, with 55.2% of subjects becoming deficient. The study revealed a significant correlation between follow-up vitamin D levels and waist circumference, hip circumference, and VAT. VAT exhibited a strong correlation not only with vitamin D levels but also with waist circumference. When analyzing gender differences, males showed a higher weight and waist-to-hip ratio, while females had higher body adiposity indexes and subcutaneous adipose tissue measurements. In conclusion, this study highlights the relationship between VAT and vitamin D levels, emphasizing the potential role of adipose tissue in vitamin D availability. Waist circumference was identified as a surrogate measure for VAT evaluation. Furthermore, the study showed variations in vitamin D response to supplementation between genders, with a higher percentage of males reaching normal vitamin D levels. Predictive factors for vitamin D levels differed between genders, with waist circumference being a significant predictor in males and body adiposity index in females.
The Acute Effect of Hydroxychloroquine Sulfate on Hunger, the Plasma Concentration of Orexigenic Peptides and Hedonic Food Intake: A Pilot Study
Authors: Ruilova Sosoranga Emily , Verbeure Wout , Geysen Hannelore , Thijs Theo , Matthys Christophe , Depoortere Inge , Tack Jan
Published: October 2023

The direct infusion of bitter solutions in the gastrointestinal tract can reduce the secretion of orexigenic hormones and influence appetite and food intake. We aimed to explore whether oral ingestion of the bitter tastant hydroxychloroquine sulfate can exert similar effects. Ten lean adult women were included in this double-blind, randomized, two-visit, crossover study. After an overnight fast, each volunteer received film-coated tablets containing 400 mg of hydroxychloroquine sulfate (Plaquenil®) or placebo. Plasma-ghrelin, -motilin, -insulin and blood-glucose concentrations were determined every 10 min before and 30 min after feeding; appetite was scored every 10 min. Hunger scores were investigated with a special interest 50–60 min after the ingestion of hydroxychloroquine sulfate, right before a rewarding chocolate milkshake was offered to drink ad libitum. Compared with the placebo, hydroxychloroquine sulfate tended to reduce hunger at the time of interest (p = 0.10). No effect was found upon subsequent milkshake intake. Motilin plasma concentrations were unaltered, but acyl-ghrelin plasma concentrations decreased after the ingestion of hydroxychloroquine sulfate (t = 40–50; p < 0.05). These data suggest that the oral intake of hydroxychloroquine sulfate tablets reduces subjective hunger via a ghrelin-dependent mechanism but does not affect motilin release, hedonic food intake or insulin levels in healthy women.
Plant-Based Diets and Risk of Hospitalization with Respiratory Infection: Results from the Atherosclerosis Risk in Communities (ARIC) Study
Authors: Kendrick Karla N. , Kim Hyunju , Rebholz Casey M. , Selvin Elizabeth , Steffen Lyn M. , Juraschek Stephen P.
Published: October 2023

The benefits of plant-based diets may depend on the type of plant. To determine the associations of healthy and unhealthy plant-based diet types on risk of hospitalization with respiratory infections or any infection, we used dietary intake data reported in a food frequency questionnaire from the Atherosclerosis Risk in Communities Study to calculate a plant-based diet index (PDI), a healthy PDI (HPDI), and an unhealthy PDI (UPDI). Cox regression was used to calculate hazard ratios for the associations of the three plant-based diet indices with the risk of hospitalization with respiratory infections and any infection-related hospitalization. Comparing the highest to lowest quintiles, HPDI was associated with a lower risk of hospitalization with respiratory infections (HR 0.86, 95% CI: 0.75, 0.99), and a lower risk of hospitalization with any infections (HR 0.87, 95% CI: 0.78, 0.97). The PDI was associated with a lower risk of hospitalization with any infections (HR 0.86, 95% CI: 0.76, 0.96). Significant associations were not observed with the UPDI. Adults with a high PDI and HPDI had a lower risk of hospitalization with any infections, whereas adults with a high HPDI had lower risk of hospitalizations with respiratory infections.
Matrigel Tunes H9 Stem Cell-Derived Human Cerebral Organoid Development
Authors: Estridge R. Chris , O’Neill Jennifer E. , Keung Albert J.
Published: October 2023

Human cerebral organoids are readily generated from human embryonic stem cells and human induced pluripotent stem cells and are useful in studying human neurodevelopment. Recent work with human cerebral organoids have explored the creation of different brain regions and the impacts of soluble and mechanical cues. Matrigel is a gelatinous, heterogenous mixture of extracellular matrix proteins, morphogens, and growth factors secreted by Engelbreth-Holm-Swarm mouse sarcoma cells. It is a core component of almost all cerebral organoid protocols, generally supporting neuroepithelial budding and tissue polarization; yet, its roles and effects beyond its general requirement in organoid protocols are not well understood, and its mode of delivery is variable, including the embedding of organoids within it or its delivery in soluble form. Given its widespread usage, we asked how H9 stem cell-derived hCO development and composition are affected by Matrigel dosage and delivery method. We found Matrigel exposure influences organoid size, morphology, and cell type composition. We also showed that greater amounts of Matrigel promote an increase in the number of choroid plexus (ChP) cells, and this increase is regulated by the BMP4 pathway. These results illuminate the effects of Matrigel on human cerebral organoid development and the importance of delivery mode and amount on organoid phenotype and composition.
Anti-Inflammatory Activity of N′-(3-(1H-indol-3-yl)benzylidene)-2-cyanoacetohydrazide Derivative via sGC-NO/Cytokine Pathway
Authors: da Silva Pablo Rayff , Apolinário Nadjaele de Melo , Silva Simone Ângela Soares da , Araruna Maria Elaine Cristina , Costa Thássia Borges , e Silva Yvnni M. S. de Medeiros , da Silva Teresinha Gonçalves , de Moura Ricardo Olímpio , dos Santos Vanda Lucia
Published: October 2023

The N-acylhydrazone function has been reported as a pharmacophore group of molecules with diverse pharmacological activities, including anti-inflammatory effects. Therefore, this study was designed to evaluate the anti-inflammatory potential of the compound N′-(3-(1H-indol-3-yl)benzylidene)-2-cyanoacetohydrazide (JR19) in vivo. The study started with the carrageenan-induced peritonitis model, followed by an investigation of leukocyte migration using the subcutaneous air pouch test and an assessment of the antinociceptive profile using formalin-induced pain. A preliminary molecular docking study focusing on the crystallographic structures of NFκB, iNOS, and sGC was performed to determine the likely mechanism of action. The computational study revealed satisfactory interaction energies with the selected targets, and the same peritonitis model was used to validate the involvement of the nitric oxide pathway and cytokine expression in the peritoneal exudate of mice pretreated with L-NAME or methylene blue. In the peritonitis assay, JR19 (10 and 20 mg/kg) reduced leukocyte migration by 59% and 52%, respectively, compared to the vehicle group, with the 10 mg/kg dose used in subsequent assays. In the subcutaneous air pouch assay, the reduction in cell migration was 66%, and the response to intraplantar formalin was reduced by 39%, particularly during the inflammatory phase, suggesting that the compound lacks central analgesic activity. In addition, a reversal of the anti-inflammatory effect was observed in mice pretreated with L-NAME or methylene blue, indicating the involvement of iNOS and sGC in the anti-inflammatory response of JR19. The compound effectively and significantly decreased the levels of IL-6, TNF-α, IL-17, and IFN-γ, and this effect was reversed in animals pretreated with L-NAME, supporting a NO-dependent anti-inflammatory effect. In contrast, pretreatment with methylene blue only reversed the reduction in TNF-α levels. Therefore, these results demonstrate the pharmacological potential of the novel N-acylhydrazone derivative, which acts through the nitric oxide pathway and cytokine signaling, making it a strong candidate as an anti-inflammatory and immunomodulatory agent.
Fibrinolytic Enzyme from Green Microalgae: A New Potential Drug for Thrombolytic Therapy?
Authors: Moura Yanara Alessandra Santana , De Souza Ariadne Tennyle Vieira , Da Costa e Silva Páblo Eugênio , Da Silva Marllyn Marques , Porto Ana Lúcia Figueiredo , Bezerra Raquel Pedrosa
Published: October 2023

Thrombosis is characterized by the pathological formation of fibrin clots within a blood vessel, leading to the obstruction of blood flow. Fibrinolytic enzymes from microorganisms have been shown to be more efficient and safer in dissolving clots. Then, this study aimed to evaluate the cell growth and fibrinolytic enzyme production of Tetradesmus obliquus under different cultivation conditions. T. obliquus grew under autotrophic and mixotrophic conditions using different concentrations of corn steep liquor (0.25 ≤ CSL ≤ 4.00%). The cells were concentrated and lysed via two different methods (sonication or homogenization) to trigger the release of the enzyme. It was precipitated via acetone or ammonium sulfate additions and purified using ion exchange chromatography. The highest biomass productivity (Px = 130 ± 12.8 mg∙L−1day−1), specific growth rate (µmax = 0.17 ± 0.00 day−1), and fibrinolytic activity (391 ± 40.0 U∙mg−1) was achieved on a mixotrophic cultivation at a 0.25% CSL concentration. The results showed that the homogenizing method had better performance in the release of enzyme, and the precipitation with acetone obtained the highest fibrinolytic activity (567 ± 49.3 U∙mg−1). The purified enzyme showed a specific activity of 1221 ± 31 U∙mg−1 and a molecular mass of 97 kDa. So, the fibrinolytic enzyme from T. obliquus had higher activity when compared to the other fibrinolytic enzymes, being a potential source for the development of therapeutic agents in thrombosis treatment. Additional studies are needed to investigate the biochemical properties and biological profile of this enzyme.
Durable Polyacrylic/Siloxane-Silica Coating for the Protection of Cast AlSi7Mg0.3 Alloy against Corrosion in Chloride Solution
Authors: Rodič Peter , Kapun Barbara , Milošev Ingrid
Published: October 2023

This study presented a novel corrosion protective coating based on polyacrylic/siloxane-silica (PEHA-SS) deposited on lightweight cast aluminium alloy AlSi7Mg0.3. The synthesis of PEHA-SS comprises organic monomer 2-ethylhexyl acrylate and organically modified silane 3-(trimethoxysilyl)propyl methacrylate as well as an inorganic silane, tetraethyl orthosilicate. The steps during the synthesis process were monitored using real-time infrared spectroscopy. The coating deposited onto the AlSi7Mg0.3 surface was characterised using various techniques, including infrared spectroscopy, 3D contact profilometry, and scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy. The corrosion resistance of the coated alloy in sodium chloride solutions was evaluated using electrochemical impedance spectroscopy. The accelerated testing of the uncoated and coated sample was performed using the Machu test. This novel, nine micrometres thick PEHA-SS coating achieved durable corrosion (barrier) protection for the AlSi7Mg0.3 alloy in 0.1 M NaCl during the first four months of immersion or under accelerated corrosion conditions in a Machu chamber containing NaCl, acetic acid, and hydrogen peroxide at 37 °C.
Religion, Social Resources, and International Migrants’ Volunteer Participation: Evidence from Yiwu City, China
Authors: Xu Tao , Zheng Kai
Published: October 2023

A large body of research has explored various factors influencing volunteer participation, particularly revealing the association between religious beliefs and volunteer engagement. However, there was relatively limited research on volunteer participation among international immigrants in China, a newly emerging immigrant country. Further investigation was needed to identify the factors influencing the volunteer engagement of international immigrants in China and the underlying mechanisms. This study analyzed data from international immigrants in Yiwu, China and found that their volunteer behavior was significantly influenced by their religious beliefs. Additionally, social resources were closely related to their volunteer participation. Furthermore, although perceived discrimination among international immigrants in China was not directly associated with volunteer participation, it promoted their religious participation and frequency, consequently encouraging their volunteer engagement. Further analysis also revealed two mechanisms through which religion impacted volunteer participation. On the one hand, religious beliefs influenced volunteer participation through the mechanism of religious indoctrination. On the other hand, though perceived discrimination among international immigrants did not directly influence volunteer participation, it did encourage them to engage in religious meetings to establish social networks for support, thereby enriching their social resources and subsequently promoting volunteer engagement. We discussed the implications of our findings for encouraging volunteer participation and for promoting the social integration of international immigrants.
Instantaneous Kinematics and Free-from-Singularity Workspace of 3-XXRRU Parallel Manipulators
Authors: Simas Henrique , Di Gregorio Raffaele , Simoni Roberto
Published: October 2023

3-XXRRU parallel manipulators (PMs) constitute a family of six-degrees-of-freedom (DOF) PMs with three limbs of type XXRRU, where R and U stand for revolute pair and universal joint, respectively, and XX indicates any actuated two-DOF mechanism that moves the axis of the first R-pair. The members of this family share the fact that they all become particular 3-RRU structures when the actuators are locked. By exploiting this feature, the present paper proposes a general approach, which holds for all the members of this family, to analyze the instantaneous kinematics, workspace, and kinetostatic performances of any 3-XXRRU PM. The results of this study include the identification of singularity conditions without reference to a specific actuation system, the proposal of two specific dimensionless performance indices ranging from 0 to 1, the determination of the optimal actuation system, and the demonstration that 3-XXRRU PMs, when appropriately sized and actuated, possess a broad singularity-free workspace that is also fully isotropic. These findings hold significance in the context of the dimensional synthesis and control of 3-XXRRU PMs. Moreover, when combined with the closed-form solutions for their positional analysis, as demonstrated in a previous publication by the same authors, 3-XXRRU PMs emerge as intriguing alternatives to other six-DOF PMs. The efficacy of the proposed approach is further illustrated through a case study.
Contributions of Climate Variability and Anthropogenic Activities to Confined Groundwater Storage in Hengshui, North China Plain
Authors: Bai Lin , Li Zhenhong , Bürgmann Roland , Zhao Yong , Jiang Liming , Cao Guoliang , Zhao Chaoying , Zhang Qin , Peng Jianbing
Published: October 2023

Groundwater storage (GWS) in confined aquifer systems is often influenced by climate variability and anthropogenic activities, and it is vital to quantify their contributions for the purpose of groundwater management and surface water allocation plans. In this study, we characterize the spatiotemporal evolution of the GWS in confined aquifer systems across Hengshui, North China Plain, and investigate its relationships with changing climate conditions and human activities through the integration of InSAR-derived surface displacements with hydraulic head observations and precipitation data, during 2004–2010 and 2016–2020. Our results indicate that the GWS in confined aquifer systems decreased markedly by 4.59 ± 0.35 km3 with an accelerating trend during the study period. The GWS variations show a strong correlation with precipitation during irrigation periods (March to July), and hence, the climate and anthropogenic-driven GWS variations can be separated from each other with a linear model. We find that the GWS depletion caused by climate variability and anthropogenic activities were −0.31 ± 0.10 km3 and −4.28 ± 0.40 km3, respectively, during the study period. The mean contribution of anthropogenic activities to the GWS variations was −71.9%, implying that the GWS variations in confined aquifer systems were primarily anthropogenic driven. It is also found that the well observations alone poorly characterize the spatiotemporal evolution of the GWS due to their limited spatial density, and the integrated InSAR/well approach appears to be promising for overcoming this challenge.
Regional-to-Local Point-Voxel Transformer for Large-Scale Indoor 3D Point Cloud Semantic Segmentation
Authors: Li Shuai , Li Hongjun
Published: October 2023

Semantic segmentation of large-scale indoor 3D point cloud scenes is crucial for scene understanding but faces challenges in effectively modeling long-range dependencies and multi-scale features. In this paper, we present RegionPVT, a novel Regional-to-Local Point-Voxel Transformer that synergistically integrates voxel-based regional self-attention and window-based point-voxel self-attention for concurrent coarse-grained and fine-grained feature learning. The voxel-based regional branch focuses on capturing regional context and facilitating inter-window communication. The window-based point-voxel branch concentrates on local feature learning while integrating voxel-level information within each window. This unique design enables the model to jointly extract local details and regional structures efficiently and provides an effective and efficient solution for multi-scale feature fusion and a comprehensive understanding of 3D point clouds. Extensive experiments on S3DIS and ScanNet v2 datasets demonstrate that our RegionPVT achieves competitive or superior performance compared with state-of-the-art approaches, attaining mIoUs of 71.0% and 73.9% respectively, with significantly lower memory footprint.
3-D Millimeter Wave Fast Imaging Technique Based on 2-D SISO/MIMO Array
Authors: Lin Bo , Yuan Yubing , Ji Yicai , Li Chao , Liu Xiaojun , Fang Guangyou
Published: October 2023

In this article, a novel three-dimensional (3-D) imaging method based on the range decomposing algorithm (RDA) is proposed for millimeter wave imaging. We combined it with binomial theory and we derive the theoretical formulation of RDA applied to single-input–single-output (SISO)/multiple-input–multiple-output (MIMO) array; meanwhile, its computational complexity and computational error are analyzed. Compared to the classical Fourier algorithm, such as the range migration algorithm (RMA) and the phase shift migration (PSM), the proposed algorithm can replace the time-consuming interpolation and accumulation operations with reasonable approximations and transformations offering a more efficient approach, while maintaining the image quality. In addition, a method based on RDA which is applicable to the transformation between MIMO and SISO, is proposed to further enhance the processing efficiency. Proof-of-principle simulation using echo data collected from a large number of antennas, verifies that the proposed algorithm has higher efficiency. In order to better verify the feasibility of the proposed algorithm, a scanning prototype located in the millimeter wave band is designed. The experimental results of different targets demonstrate that the proposed algorithm achieves significantly higher reconstruction efficiency when compared to the traditional algorithms.
Using the Commercial GNSS RO Spire Data in the Neutral Atmosphere for Climate and Weather Prediction Studies
Authors: Ho Shu-peng , Zhou Xinjia , Shao Xi , Chen Yong , Jing Xin , Miller William
Published: October 2023

Recently, the NOAA has included GNSS (Global Navigation Satellite System) Radio Occultation (RO) data as one of the crucial long-term observables for weather and climate applications. To include more GNSS RO data in its numerical weather prediction systems, the NOAA Commercial Weather Data Pilot program (CWDP) started to explore the commercial RO data available on the market. After two rounds of pilot studies, the CWDP decided to award the first Indefinite Delivery Indefinite Quantity (IDIQ) contract to GeoOptics and Spire Incs. in 2020. This study examines the quality of Spire RO data products for weather and climate applications. Spire RO data collected from commercial CubeSats are carefully compared with data from Formosa Satellite Mission 7–Constellation Observing System for Meteorology, Ionosphere, and Climate-2 (COSMIC-2), the fifth-generation European Centre for Medium-Range Weather Forecasts (ECMWF) atmospheric reanalysis (ERA5), and high-quality radiosonde data. The results demonstrate that, despite their generally lower Signal-Noise-Ratio (SNR), Spire RO data show a pattern of lowest penetration height similar to that of COSMIC-2. The Spire and COSMIC-2 penetration heights are between 0.6 and 0.8 km altitude over tropical oceans. Although using different GNSS RO receivers, the precision of Spire STRATOS receivers is of the same quality as those of the COSMIC-2 TriG (Global Positioning System—GPS, GALILEO, and GLObal NAvigation Satellite System—GLONASS) RO Receiver System (TGRS) receivers. Furthermore, the Spire and COSMIC-2 retrieval accuracies are quite comparable. We validate the Spire temperature and water vapor profiles by comparing them with collocated radiosonde observation (RAOB) data. Generally, over the height region between 8 km and 16.5 km, the Spire temperature profiles match those from RS41 RAOB very well, with temperature biases of <0.02 K. Over the height range from 17.8 to 26.4 km, the temperature differences are ~−0.034 K, with RS41 RAOB being warmer. We also estimate the error covariance matrix for Spire, COSMIC-2, and KOMPSAT-5. The results show that the COSMIC-2 estimated error covariance values are slightly more significant than those from Spire over the oceans at the mid-latitudes (45°N–30°N and 30°S–45°S), which may be owing to COSMIC-2 SNR being relatively lower at those latitudinal zones.