bluuebunny/crossref_metadata_2025 huggingface dataset:
Feasibility and Safety of Perioperative Chemotherapy With Fluorouracil Plus Leucovorin, Oxaliplatin, and Docetaxel for Locally Advanced Gastric Cancer Patients in China
Authors: Sah Birendra Kumar , Xu Wei , Zhang Benyan , Zhang Huan , Yuan Fei , Li Jian , Liu Wentao , Yan Chao , Li Chen , Yan Min , Zhu Zhenggang
Published: January 2021

Background

Neoadjuvant fluorouracil plus leucovorin, oxaliplatin, and docetaxel (FLOT) has shown significant benefits for gastric cancer patients. However, it has not been well accepted in Asian countries. We conducted a prospective study on the safety and feasibility of the FLOT regimen in Chinese patients.

Methods

Patients with adenocarcinoma of the stomach or esophagogastric junction received four cycles of neoadjuvant chemotherapy (NAC) and four cycles of adjuvant chemotherapy (AC) with the FLOT regimen. The completion status of chemotherapy, adverse events, postoperative morbidities, and pathological tumor regression were analyzed. The 2-year overall survival (OS) and relapse-free survival are presented.

Results

Altogether, 10 patients were enrolled, and all patients completed four cycles of neoadjuvant chemotherapy. There were no severe hematological adverse events (grade 3 or above), except for a case of grade 3 anemia. All 10 patients underwent radical gastrectomy. Nine patients had R0 resection, and three patients had complete or subtotal pathological tumor regression. Nine patients completed four cycles of adjuvant chemotherapy, but only one patient completed the full dose of adjuvant chemotherapy. The dose of adjuvant chemotherapy was reduced by 25% or less in the other patients. The median follow-up time was 23.13 months, eight patients achieved the overall survival endpoint, and seven patients had relapse-free survival for this period. Two patients died of disease progression.

Conclusions

Our study demonstrates that the neoadjuvant FLOT regimen is safe and effective for Chinese patients. Dose adjustment is necessary for adjuvant chemotherapy. The pathological regression and survival rates need reevaluation in a larger cohort. The trial is registered with ClinicalTrials.gov (number NCT03646591).
Fully Automatic Sliding Motion Compensated and Simultaneous 4D-CBCT via Bilateral Filtering
Authors: Dang Jun , You Tao , Sun Wenzheng , Xiao Hanguan , Li Longhao , Chen Xiaopin , Dai Chunhua , Li Ying , Song Yanbo , Zhang Tao , Chen Deyu
Published: January 2021

Purpose

To incorporate the bilateral filtering into the Deformable Vector Field (DVF) based 4D-CBCT reconstruction for realizing a fully automatic sliding motion compensated 4D-CBCT.

Materials and Methods

Initially, a motion compensated simultaneous algebraic reconstruction technique (mSART) is used to generate a high quality reference phase (e.g. 0% phase) by using all phase projections together with the initial 4D-DVFs. The initial 4D-DVF were generated via Demons registration between 0% phase and each other phase image. The 4D-DVF will then kept updating by matching the forward projection of the deformed high quality 0% phase with the measured projection of the target phase. The loss function during this optimization contains an projection intensity difference matching criterion plus a DVF smoothing constrain term. We introduce a bilateral filtering kernel into the DVF constrain term to estimate the sliding motion automatically. The bilateral filtering kernel contains three sub-kernels: 1) an spatial domain Guassian kernel; 2) an image intensity domain Guassian kernel; and 3) a DVF domain Guassian kernel. By choosing suitable kernel variances, the sliding motion can be extracted. A non-linear conjugate gradient optimizer was used. We validated the algorithm on a non-uniform rotational B-spline based cardiac-torso (NCAT) phantom and four anonymous patient data. For quantification, we used: 1) the Root-Mean-Square-Error (RMSE) together with the Maximum-Error (MaxE); 2) the Dice coefficient of the extracted lung contour from the final reconstructed images and 3) the relative reconstruction error (RE) to evaluate the algorithm's performance.

Results

For NCAT phantom, the motion trajectory's RMSE/MaxE are 0.796/1.02 mm for bilateral filtering reconstruction; and 2.704/4.08 mm for original reconstruction. For patient pilot study, the 4D-Dice coefficient obtained with bilateral filtering are consistently higher than that without bilateral filtering. Meantime several image content such as the rib position, the heart edge definition, the fibrous structures all has been better corrected with bilateral filtering.

Conclusion

We developed a bilateral filtering based fully automatic sliding motion compensated 4D-CBCT scheme. Both digital phantom and initial patient pilot studies confirmed the improved motion estimation and image reconstruction ability. It can be used as a 4D-CBCT image guidance tool for lung SBRT treatment.
Digital Simulation Improves, Maintains, and Helps Transfer Health-Care Providers' Neonatal Resuscitation Knowledge
Authors: Ghoman Simran K. , Cutumisu Maria , Schmölzer Georg M.
Published: January 2021

Purpose:To safely care for their newborn patients, health-care professionals (HCP) must undergo frequent training to improve and maintain neonatal resuscitation knowledge and skills. However, the current approach to neonatal resuscitation simulation training is time and resource-intensive, and often inaccessible. Digital neonatal resuscitation simulation may present a convenient alternative for more frequent training.

Method:Fifty neonatal HCPs participated in the study (44 female; 27 nurses, 3 nurse practitioners, 14 respiratory therapists, 6 doctors). This study was conducted at a tertiary perinatal center in Edmonton, Canada from April–August 2019, with 2-month (June–October 2019) and 5-month (September 2019–January 2020) follow-up. Neonatal HCPs were recruited by volunteer sampling to complete a demographic survey, pre-test (baseline knowledge), two digital simulation scenarios (intervention), and post-test (knowledge acquisition). Two months later, participants repeated the post-test (knowledge retention). Five months after the initial intervention, participants completed a post-test using a table-top simulation (knowledge transfer). Longitudinal analyses were used to compare participants' performance over time.

Results:Overall the proportion of correct performance increased: 21/50 (42%) passed the pre-test, 39/50 (78%) the post-test, 30/43 (70%) the 2-month post-test, and 32/40 (80%) the 5-month post-test. GLMM and GEE analyses revealed that performance on all post-tests was significantly better than the performance on the pre-test. Therefore, training with the RETAIN digital simulation effectively improves, maintains, and transfers HCPs' neonatal resuscitation knowledge.

Conclusions:Digital simulation improved, maintained, and helped transfer HCPs' neonatal resuscitation knowledge over time. Digital simulation presents a promising approach for frequent neonatal resuscitation training, particularly for distance-learning applications.
Grindelia squarrosa Extract and Grindelic Acid Modulate Pro-inflammatory Functions of Respiratory Epithelium and Human Macrophages
Authors: Gierlikowska Barbara , Filipek Agnieszka , Gierlikowski Wojciech , Kania Dominika , Stefańska Joanna , Demkow Urszula , Kiss Anna K.
Published: January 2021

Aim of the study: Both nasal and bronchial epithelial cells have evolved sophisticated mechanisms involved in cellular response to bacterial infection. Recognition of pathogens by TLR receptors activate the NF-κB transcription factor, and lead to production of wide spectrum of cytokines (TNF-α, IL-1β, IL-6 and IL-8). Released by epithelium proinflammatory cytokines intensify migration of macrophages to damaged tissues and modulate their pro-inflammatory functions. Based on traditional use of G. squarrosa aerial parts we hypothesized that successful treatment of cold-related diseases may arise from modulation of the pro-inflammatory functions of respiratory epithelium and human monocytes/macrophages. The biological activity of G. squarrosa extract and grindelic acid were compared with clarithromycin and budesonide used as positive controls.

Methods: The expression of surface receptors (TLR-4, IL-10) and expression of adhesive molecules (ICAM-1, VCAM-1, E-selectin) was analyzed with flow cytometry. The macrophage attachment to the epithelial cells was assessed fluorimetrically. The p65 NF-κB concentration and cytokine production was measured spectrophotometrically using enzyme-linked immunosorbent assay. Antibacterial activity was examined by the standard disc-diffusion method and serial dilution method according to CLSI guidelines.

Results:G. squarrosa extract and grindelic acid had no antimicrobial effect. However, we noticed significant modulation of pro-inflammatory functions of LPS-stimulated nasal and bronchial epithelium. G. squarrosa extract treatment resulted in decrease of TLR-4 expression and p65 NF-κB concentration and inhibition of cytokines synthesis (IL-8, TNF-α, IL-1β and IL-6) in both cellular models. Additionally, G. squarrosa extract slightly modulated ICAM-1 expression affecting on attachment of macrophages to epithelium. Only G. squarrosa extract was able to stimulate the anti-inflammatory functions of macrophages by inducing TGF-β release and IL-10 receptor surface expression. Grindelic acid, identified as a dominant compound in the plant extract, modulated pro-inflammatory functions of epithelium and macrophages slightly.

Conclusion: The obtained results support traditional use of Grindelia squarrosa preparations for a treatment cold-associated diseases symptoms. In our opinion, the observed biological effect of extract may be a consequence of synergistic effect of all compounds present in the extract.
Dynamical Analysis of a Mathematical Model of COVID-19 Spreading on Networks
Authors: Li Wang , Fu Xinjie , Sun Yongzheng , Liu Maoxing
Published: January 2021

In this article, an SEAIRS model of COVID-19 epidemic on networks is established and analyzed. Following the method of the next-generation matrix, we derive the basic reproduction number R0, and it shows that the asymptomatic infector plays an important role in disease spreading. We analytically show that the disease-free equilibrium E0 is asymptotically stable if R0≤1; moreover, the effects of various quarantine strategies are investigated and compared by numerical simulations. The results obtained are informative for us to further understand the asymptomatic infector in COVID-19 propagation and get some effective strategies to control the disease.
Age- and Sex-Differences in Cardiac Characteristics Determined by Echocardiography in Masters Athletes
Authors: Wooten Savannah V. , Moestl Stefan , Chilibeck Phil , Alvero Cruz José Ramón , Mittag Uwe , Tank Jens , Tanaka Hirofumi , Rittweger Jörn , Hoffmann Fabian
Published: January 2021

Background

Cardiac function and morphology are known to differ between men and women. Sex differences seen with echocardiography have not been studied systematically in masters athletes.

Purpose

To evaluate sex differences in cardiac structure, function and left ventricular (LV) systolic global longitudinal strain among masters athletes.

Methods

This cross-sectional study comprises of 163 masters athletes (M= 109, 60 ± 12 years;F= 55, 57 ± 12 years, range 36–91 years) who participated at the 23rd World Masters Athletics Championship held in Málaga, Spain. All athletes underwent state-of-the-art echocardiography including cardiac function, morphology, strain and hemodynamic assessment.

Results

Left ventricular mass was higher in male than in female athletes (174 ± 44 vs. 141 ± 36g,p< 0.01) due to greater end-diastolic intraventricular septal, LV posterior wall and LV basal diameter. However, LV mass index did not differ between the groups. End-diastolic LV volume and right ventricular area, both indexed to body-surface-area, were greater in men than in women (52.8 ± 11.0 vs. 46.1 ± 8.5 ml/m2,p< 0.01, 9.5 ± 2.4 vs. 8.1 ± 1.7 cm2/m2,p< 0.01). In contrast, women had higher LV systolic global longitudinal strain (-20.2 ± 2.6 vs. -18.8 ± 2.6%,p< 0.01) and LV outflow tract flow velocity (75.1 ± 11.1 vs. 71.2 ± 11.1 cm/s,p= 0.04). Systolic and diastolic blood pressure, LV ejection fraction, and stroke volume index were not different between sexes.

Conclusion

Cardiac sex differences are present even among masters athletes. Lifelong exercise training does not appear to exasperate morphological difference to a point of cardiac risk or dysfunction in both male and female athletes.
Cytosolic Free N-Glycans Are Retro-Transported Into the Endoplasmic Reticulum in Plant Cells
Authors: Katsube Makoto , Ebara Natsuki , Maeda Megumi , Kimura Yoshinobu
Published: January 2021

During endoplasmic reticulum (ER)-associated degradation, free N-glycans (FNGs) are produced from misfolded nascent glycoproteins via the combination of the cytosolic peptide N-glycanase (cPNGase) and endo-β-N-acetylglucosaminidase (ENGase) in the plant cytosol. The resulting high-mannose type (HMT)-FNGs, which carry one GlcNAc residue at the reducing end (GN1-FNGs), are ubiquitously found in developing plant cells. In a previous study, we found that HMT-FNGs assisted in protein folding and inhibited β-amyloid fibril formation, suggesting a possible biofunction of FNGs involved in the protein folding system. However, whether these HMT-FNGs occur in the ER, an organelle involved in protein folding, remained unclear. On the contrary, we also reported the presence of plant complex type (PCT)-GN1-FNGs, which carry the Lewisa epitope at the non-reducing end, indicating that these FNGs had been fully processed in the Golgi apparatus. Since plant ENGase was active toward HMT-N-glycans but not PCT-N-glycans that carry β1-2xylosyl and/or α1-3 fucosyl residue(s), these PCT-GN1-FNGs did not appear to be produced from fully processed glycoproteins that harbored PCT-N-glycans via ENGase activity. Interestingly, PCT-GN1-FNGs were found in the extracellular space, suggesting that HMT-GN1-FNGs formed in the cytosol might be transported back to the ER and processed in the Golgi apparatus through the protein secretion pathway. As the first step in elucidating the production mechanism of PCT-GN1-FNGs, we analyzed the structures of free oligosaccharides in plant microsomes and proved that HMT-FNGs (Man9-7GlcNAc1 and Man9-8GlcNAc2) could be found in microsomes, which almost consist of the ER compartments.
Etiology of Cyclocarya paliurus Anthracnose in Jiangsu Province, China
Authors: Zheng Xiang-rong , Zhang Mao-jiao , Shang Xu-lan , Fang Sheng-zuo , Chen Feng-mao
Published: January 2021

Cyclocarya paliurus is an extremely valuable and multifunctional tree species whose leaves have traditionally been used in used in medicine or as a medicinal tea in China. In recent years, anthracnose has been frequently observed on young leaves of C. paliurus in several nurseries located in Jiangsu Province, resulting in great yield and quality losses. To date, no information is available about the prevalence of C. paliurus anthracnose in China. The main purpose of the present study was to characterize the etiology of C. paliurus anthracnose. Phylogenetic analysis of the eight-loci concatenated dataset revealed that all 44 single-spore Colletotrichum isolates belonged to three species in the Colletotrichum gloeosporioides species complex, namely, Colletotrichum aenigma, Colletotrichum fructicola, and C. gloeosporioides sensu stricto. Phenotypic features, including the colony appearance and the morphology of conidia, appressoria, and ascospores, were consistent with the phylogenetic grouping. Virulence tests validated that the three Colletotrichum species could cause typical symptoms of anthracnose on C. paliurus leaves, similar to those observed in the field. The optimum mycelial growth temperature ranged from 25 to 30°C for all representative isolates, while C. gloeosporioides s. s. isolates exhibited greater tolerance to high temperature (40°C). Fungicide sensitivity assays indicated that all three Colletotrichum species were sensitive to tetramycin, which may be a potential alternative for the management of C. paliurus anthracnose. To our knowledge, this study provides the first report of C. aenigma, C. fructicola, and C. gloeosporioides s. s. causing C. paliurus anthracnose in China as well as in the world.
Self-Efficacy Between Previous and Current Mathematics Performance of Undergraduate Students: An Instrumental Variable Approach to Exposing a Causal Relationship
Authors: Zakariya Yusuf F.
Published: January 2021

Purpose

Self-efficacy has been argued theoretically and shown empirically to be an essential construct for students’ improved learning outcomes. However, there is a dearth of studies on its causal effects on performance in mathematics among university students. Meanwhile, it will be erroneous to assume that results from other fields of studies generalize to mathematics learning due to the task-specificity of the construct. As such, attempts are made in the present study to provide evidence for a causal relationship between self-efficacy and performance with a focus on engineering students following a mathematics course at a Norwegian university.

Method

The adopted research design in the present study is a survey type in which collected data from first-year university students are analyzed using structural equation modeling with weighted least square mean and variance adjusted (WLSMV) estimator. Data were generated using mainly questionnaires, a test of prior mathematics knowledge, and the students’ final examination scores in the course. The causal effect of self-efficacy was discerned from disturbance effects on performance by using an innovative instrumental variable approach to structural equation modeling.

Results

The findings confirmed a significant direct effect of the prior mathematics knowledge test (β = 0.52, SE = 0.01, p < 0.001) on self-efficacy, a significant direct effect (β = 0.43, SE = 0.19, p = 0.02) of self-efficacy on performance, and a substantial mediating effect (β = 0.22, SE = 0.10, p = 0.03) of self-efficacy between a prior mathematics knowledge test and performance. Prior mathematics knowledge and self-efficacy explained 30% variance of the performance. These findings are interpreted to be substantial evidence for the causal effect of self-efficacy on students’ performance in an introductory mathematics course.

Conclusion

The findings of the present study provide empirically supports for designing self-efficacy interventions as proxies to improve students’ performance in university mathematics. Further, the findings of the present study confirm some postulates of Bandura’s agentic social cognitive theory.
Shared Leadership and Team Effectiveness: An Investigation of Whether and When in Engineering Design Teams
Authors: Wu Qiong , Cormican Kathryn
Published: January 2021

Shared leadership is lauded to be a performance-enhancing approach with applications in many management domains. It is conceptualized as a dynamic team process as it evolves over time. However, it is surprising to find that there are no studies that have examined its temporally relevant boundary conditions for the effectiveness of the team. Contributing to an advanced understanding of the mechanism of shared leadership in engineering design teams, this research aims to investigate whether shared leadership is positively related to team effectiveness and when shared leadership is more likely to be effective. Using a field sample of 119 individuals in 26 engineering design teams from China and the technique of social network analysis, we found that, consistent with cognate studies, shared leadership is positively related to team effectiveness when measured in terms of team task performance and team viability. Moreover, by integrating the project life cycle as a moderator, this study is among the first to investigate the temporal factors, for the effectiveness of shared leadership. The result shows that the stage of the project life cycle moderates the positive shared leadership-team effectiveness relationship, such that this association is stronger at the early phase than at the later phase of the project. Overall, these findings offer insightful thoughts to scholars in the field of shared leadership and bring practical suggestions for project managers in business who seek to implement best practice in organizations toward high team effectiveness.
Sensory Stimulation of Oxytocin Release Is Associated With Stress Management and Maternal Care
Authors: Takahashi Toku
Published: January 2021

It has been shown that various types of stress initiate different physiological and neuroendocrine disorders. Oxytocin (OT) is mainly produced in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) of the hypothalamus. Hypothalamic OT has antistress effects and attenuates the hypothalamic–pituitary–adrenal (HPA) axis. One mechanism behind the antistress effects of OT is mediated through the inhibition from GABAA receptors on corticotropin-releasing factor (CRF) expression at the PVN. Various manual therapies such as acupuncture, transcutaneous electrical nerve stimulation (TENS), and massage initiate the stimulation of somatosensory neurons of the body. It is well-known that TENS simulates OT expression, while it inhibits CRF expression at the PVN following chronic stress loading in rodents. Upregulation of OT expression at the hypothalamus is activated by the somatosensory stimulation, which is mediated via the spinothalamic pathway (the connection between the spinal cord and hypothalamus). Thus, somatosensory stimulation is beneficial in treating stress-associated symptoms. Hypothalamic OT is associated with the social behaviors, including maternal care and affiliation. Childhood neglect and/or child abuse are severely responsible for deleterious long-term effects on the cognitive/social activity and behavioral development. At parturition, a profound amount of OT is released into the systemic circulation in response to vaginal and cervical stimulation caused by the body of fetus, which induces the onset of maternal behavior. Peridural anesthesia effectively impairs the sensitivity to vaginal and cervical stimulation at parturition. OT levels in cerebrospinal fluid is significantly reduced following peridural anesthesia. The vaginal delivery mothers had significantly more OT pulses than the caesarian section (CS) mothers. Due to low levels of endogenous OT, maternal behavior could be interrupted by epidural anesthesia and CS at parturition because of the reduction of the usual sensory input from the genitalia.
Emotivity in the Voice: Prosodic, Lexical, and Cultural Appraisal of Complaining Speech
Authors: Mauchand Maël , Pell Marc D.
Published: January 2021

Emotive speech is a social act in which a speaker displays emotional signals with a specific intention; in the case of third-party complaints, this intention is to elicit empathy in the listener. The present study assessed how the emotivity of complaints was perceived in various conditions. Participants listened to short statements describing painful or neutral situations, spoken with a complaining or neutral prosody, and evaluated how complaining the speaker sounded. In addition to manipulating features of the message, social-affiliative factors which could influence complaint perception were varied by adopting a cross-cultural design: participants were either Québécois (French Canadian) or French and listened to utterances expressed by both cultural groups. The presence of a complaining tone of voice had the largest effect on participant evaluations, while the nature of statements had a significant, but smaller influence. Marginal effects of culture on explicit evaluation of complaints were found. A multiple mediation analysis suggested that mean fundamental frequency was the main prosodic signal that participants relied on to detect complaints, though most of the prosody effect could not be linearly explained by acoustic parameters. These results highlight a tacit agreement between speaker and listener: what characterizes a complaint is how it is said (i.e., the tone of voice), more than what it is about or who produces it. More generally, the study emphasizes the central importance of prosody in expressive speech acts such as complaints, which are designed to strengthen social bonds and supportive responses in interactive behavior. This intentional and interpersonal aspect in the communication of emotions needs to be further considered in research on affect and communication.
A German Version of the Staff Attitude to Coercion Scale. Development and Empirical Validation
Authors: Efkemann Simone A. , Scholten Matthé , Bottlender Ronald , Juckel Georg , Gather Jakov
Published: January 2021

Background:Individual staff factors, such as personality traits and attitudes, are increasingly seen as an important factor in the reduction of coercion in mental health services. At the same time, only a few validated instruments exist to measure those factors and examine their influence on the use of coercion.

Aim:The present study aimed to develop and validate a German version of the Staff Attitude to Coercion Scale (SACS).

Methods:The original English version of the SACS published was translated into German. Subsequently, it was empirically validated on a sample ofN= 209 mental health professionals by conducting an exploratory factor analysis.

Results:The three-factor structure in the original version of the SACS, consisting of critical, pragmatic and positive attitudes toward the use of coercion, could not be replicated. Instead, the German version revealed one factor ranging from rejecting to approving the use of coercion.

Conclusion:The SACS is one of the first instruments created to assess staff attitudes toward coercion in a validated way. The version of the instrument developed in this study allows for a validated assessment of those attitudes in German. Our results highlight the ethical importance of using validated measurements in studies on the role of staff factors in the reduction of coercion.
Associations of Substance Use Behaviors With Suicidal Ideation and Suicide Attempts Among US and Chinese Adolescents
Authors: Guo Lan , Wang Wanxin , Du Xueying , Guo Yangfeng , Li Wenyan , Zhao Meijun , Wu Ruipeng , Lu Ciyong
Published: January 2021

Background: Adolescence has been described as a period of increased health risk-taking behaviors. Given the variety of cultural contexts, healthcare systems, and public health policies in different regions, the present study aimed to determine whether there are similar or different associations of substance use behaviors with suicidal ideation and suicide attempts among US and Chinese adolescents.

Methods: This study included a total of 14,765 US adolescents from the 2017 National Youth Risk Behavior Surveillance System (YRBSS) and 24,345 Chinese adolescents from the 2017 School-based Chinese Adolescents Health Survey (SCAHS).

Results: The proportions of suicidal ideation and suicide attempts were 17.4 and 5.7% among US adolescents, which were higher than those among Chinese adolescents (suicidal ideation: 13.7% and suicide attempts: 2.7%). Among Chinese adolescents, the most common substance use behavior was “alcohol use (55.4%),” followed by “cigarette use (11.6%).” Among US adolescents, the most popular substance was alcohol (ever used: 55.9%), followed by marijuana (ever used: 34.6%). Moreover, alcohol use was significantly related to suicidal ideation/suicide attempts only in Chinese adolescents [suicidal ideation: Adjusted odds ratio (AOR) = 1.88, 95% CI = 1.71~2.06; suicide attempts: AOR = 2.12, 95% CI = 1.71~2.63], and marijuana use was associated with suicidal ideation and suicide attempts only in the US adolescent group (suicidal ideation: AOR = 1.23, 95% CI = 1.06~1.44; suicide attempts: AOR = 1.51, 95% CI = 1.21~1.87). Moreover, although the associations of prescription pain medication use with suicide attempts were significant in both Chinese and US adolescent groups, the adjusted associations were stronger in Chinese adolescents than in US adolescents (Chinese adolescents: AOR = 3.97, 95% CI = 2.76~5.72; US adolescents: AOR = 1.76, 95% CI = 1.43~2.16; P < 0.05).

Conclusions: The associations of alcohol use with suicidal ideation and suicide attempts were only significant in Chinese adolescents. Marijuana use was associated with suicidal ideation and suicide attempts only in the US adolescent group. Although the associations of prescription pain medication use with suicide attempts were significant in both Chinese and US adolescent groups, the adjusted associations were significantly stronger for Chinese adolescents. These findings might be related to the differences in cultural contexts, healthcare systems, and public health policies in the two different countries.
Multi-Morbidity in the Older Person: An Examination of Polypharmacy and Socioeconomic Status
Authors: Nwadiugwu Martin C.
Published: January 2021

There has been increased focus on clinically managing multi-morbidity in the older population, but it can be challenging to find appropriate paradigm that addresses the socio-economic burden and risk for polypharmacy. The Commission on Social Determinants of Health (CSDH) has examined the need for institutional change and the parallel need to address the social causes of poor health. This study explored three potential interventions namely, meaningful information from electronic health records (EHR), social prescribing, and redistributive welfare policies from a person-centered perspective using the CARE (connecting, assessing, responding, and empowering) approach. Economic instruments that immediately redistribute state welfare and reduce income disparity such as direct taxation and conditional cash transfers could be adopted to enable older people with long-term conditions have access to healthcare services. Decreased socioeconomic inequality and unorthodox prescriptive interventions that reduce polypharmacy could mitigate barriers to effectively manage the complexities of multi-morbidity.
AI Data-Driven Personalisation and Disability Inclusion
Authors: Wald Mike
Published: January 2021

This study aims to help people working in the field of AI understand some of the unique issues regarding disabled people and examines the relationship between the terms “Personalisation” and “Classification” with regard to disability inclusion. Classification using big data struggles to cope with the individual uniqueness of disabled people, and whereas developers tend to design for the majority so ignoring outliers, designing for edge cases would be a more inclusive approach. Other issues that are discussed in the study include personalising mobile technology accessibility settings with interoperable profiles to allow ubiquitous accessibility; the ethics of using genetic data-driven personalisation to ensure babies are not born with disabilities; the importance of including disabled people in decisions to help understand AI implications; the relationship between localisation and personalisation as assistive technologies need localising in terms of language as well as culture; the ways in which AI could be used to create personalised symbols for people who find it difficult to communicate in speech or writing; and whether blind or visually impaired person will be permitted to “drive” an autonomous car. This study concludes by suggesting that the relationship between the terms “Personalisation” and “Classification” with regards to AI and disability inclusion is a very unique one because of the heterogeneity in contrast to the other protected characteristics and so needs unique solutions.
Coupled Biotic-Abiotic Processes Control Biogeochemical Cycling of Dissolved Organic Matter in the Columbia River Hyporheic Zone
Authors: Fudyma Jane D. , Chu Rosalie K. , Graf Grachet Nathalia , Stegen James C. , Tfaily Malak M.
Published: January 2021

A critical component of assessing the impacts of climate change on watershed ecosystems involves understanding the role that dissolved organic matter (DOM) plays in driving whole ecosystem metabolism. The hyporheic zone—a biogeochemical control point where ground water and river water mix—is characterized by high DOM turnover and microbial activity and is responsible for a large fraction of lotic respiration. Yet, the dynamic nature of this ecotone provides a challenging but important environment to parse out different DOM influences on watershed function and net carbon and nutrient fluxes. We used high-resolution Fourier-transform ion cyclotron resonance mass spectrometry to provide a detailed molecular characterization of DOM and its transformation pathways in the Columbia river watershed. Samples were collected from ground water (adjacent unconfined aquifer underlying the Hanford 300 Area), Columbia river water, and its hyporheic zone. The hyporheic zone was sampled at five locations to capture spatial heterogeneity within the hyporheic zone. Our results revealed that abiotic transformation pathways (e.g., carboxylation), potentially driven by abiotic factors such as sunlight, in both the ground water and river water are likely influencing DOM availability to the hyporheic zone, which could then be coupled with biotic processes for enhanced microbial activity. The ground water profile revealed high rates of N and S transformations via abiotic reactions. The river profile showed enhanced abiotic photodegradation of lignin-like molecules that subsequently entered the hyporheic zone as low molecular weight, more degraded compounds. While the compounds in river water were in part bio-unavailable, some were further shown to increase rates of microbial respiration. Together, river water and ground water enhance microbial activity within the hyporheic zone, regardless of river stage, as shown by elevated putative amino-acid transformations and the abundance of amino-sugar and protein-like compounds. This enhanced microbial activity is further dependent on the composition of ground water and river water inputs. Our results further suggest that abiotic controls on DOM should be incorporated into predictive modeling for understanding watershed dynamics, especially as climate variability and land use could affect light exposure and changes to ground water essential elements, both shown to impact the Columbia river hyporheic zone.
Multi-Scape Interventions to Match Spatial Scales of Demand and Supply of Ecosystem Services
Authors: Laca Emilio A.
Published: January 2021

The original focus on supply of ecosystem services is shifting toward matching supply and demand. This new focus underlines the need to consider not only the amount of ecosystem services but also their spatial and temporal distributions relative to demand. Ecosystem functions and services have characteristic or salient scales that are defined by the scales at which the producing organisms or communities exist and function. Provision of ecosystem services (ES) and functions can be managed optimally by controlling the spatio-temporal distribution of landscape and community components. A simple model represents distributions of ES as kernels centered at the location of interventions such as grassland restoration or establishment of nesting habitat for pollinators. Distribution kernels allow non-habitat patches to receive ecosystem services from species they cannot support. Simulations for three contrasting ES producing organisms (bumblebees, Northern Harriers, and oak trees) show the effects of interacting distribution of interventions and demand for ES. More ES demand is met when the intervention is spread out in the landscape and demand is evenly distributed, particularly when the kernel radius is much larger than the minimum intervention required for the ES producing unit to be established. Because different functions have different reaches and saturation points, the level of ES demand met at any point in space can be modulated by controlling the spatial distribution of landscape components created by interventions. Different ES can be promoted by the same type and quantity of intervention by controlling the continuum of scales in the distribution of interventions. This work provides a conceptual and quantitative basis to consider the spatial design of interventions to match ES supply and demand.
A Scoping Review of the Evidence for the Medicinal Use of Natural Honey in Animals
Authors: Vogt Nadine A. , Vriezen Ellen , Nwosu Andrea , Sargeant Jan M.
Published: January 2021

Honey has a history of medicinal use that predates written records. In recent decades, there has been renewed interest in the use of honey in human medicine, particularly for the treatment of burns and other wounds. Several recent systematic reviews in the human literature have demonstrated the efficacy of honey in the treatment of a number of conditions, including burns, wounds and oral mucositis. The goal of this scoping review was to describe the nature and extent of the current body of evidence addressing the medicinal use of natural honey and/or its derivatives in animals. Although the focus of this review was the veterinary literature, all animal species except insects and humans were eligible, including animals used for biomedical research. Electronic databases searched were MEDLINE, CAB Abstracts, AGRICOLA, Web of Science Core Collection, and Web of Science SciELO Citation Index. A total of 397 articles reporting 436 primary research studies were included in this review. The majority of the articles were biomedical research articles (n = 350); fewer veterinary research articles were identified (n = 47). Apart from one systematic review, all biomedical studies were challenge trials. Most veterinary studies were case reports/series (n = 23), followed by challenge trials (n = 18) and controlled trials (n = 8). The animal species examined within veterinary articles consisted primarily of dogs, horses, cats and cattle, whereas the majority of biomedical research articles examined rats and mice. Wound healing was the most common indication examined; other indications examined included the prevention or treatment of gastric ulcers, bacterial and parasitic infections, toxic exposures, metabolic conditions (e.g., diabetes) and neoplasia. The majority of interventions consisted of non-medical grade honey (n = 412/436), followed by medical-grade honey (n = 29/436) and derivatives of natural honey (n = 9/436). With much of the current veterinary literature consisting of case reports and case series, high-quality primary veterinary research in the form of controlled trials or challenge trials is needed to advance this field, as well as to provide sound data for evidence-based assessments of the efficacy of honey in clinical veterinary practise.
Advanced Construction of the Dynamic Matrix in Numerically Efficient Fuzzy MPC Algorithms
Authors: Marusak Piotr M.
Published: January 2021

A method for the advanced construction of the dynamic matrix for Model Predictive Control (MPC) algorithms with linearization is proposed in the paper. It extends numerically efficient fuzzy algorithms utilizing skillful linearization. The algorithms combine the control performance offered by the MPC algorithms with nonlinear optimization (NMPC algorithms) with the numerical efficiency of the MPC algorithms based on linear models in which the optimization problem is a standard, easy-to-solve, quadratic programming problem with linear constraints. In the researched algorithms, the free response obtained using a nonlinear process model and the future trajectory of the control signals is used to construct an advanced dynamic matrix utilizing the easy-to-obtain fuzzy model. This leads to obtaining very good prediction and control quality very close to those offered by NMPC algorithms. The proposed approach is tested in the control system of a nonlinear chemical control plant—a CSTR reactor with the van de Vusse reaction.
Shoot Girdling Improves Rooting Performance of Kalamata Olive Cuttings by Upregulating Carbohydrates, Polyamines and Phenolic Compounds
Authors: Denaxa Nikoleta-Kleio , Vemmos Stavros N. , Roussos Peter A.
Published: January 2021

Girdling (a ring of bark approximately 5 mm wide) was applied on olive mother plants to investigate its effect on the rooting ability of cuttings. Treatment was applied in autumn and in spring. The cuttings were then immersed for 5 s into 2000 mg L−1 indole-3-butyric acid (IBA) in a 45% v/v ethanolic solution. Thirty days after girdling, cuttings were taken from girdled and control shoots from the part just above the girdle zone (basal), as well as from the part right above (middle). The base of the cuttings was collected, and the concentration of polyamines, phenolic compounds and soluble carbohydrates was determined. Girdling enhanced the rooting performance of the cuttings, while autumn proved to be a better season for rooting compared to spring. Girdling positively affected the concentration of all the measured parameters. The main characteristics of autumn cuttings were the high levels of glucose, mannitol, free and total polyamines, hydroxytyrosol, tyrosol, verbascoside, oleuropein, quercetin and luteolin. The cuttings taken from the middle part of the girdled shoot exhibited high sucrose, glucose, mannitol, free polyamines, hydroxytyrosol, luteolin-7-glucoside, total phenols and flavanol concentrations. Nonetheless, further research is needed in order to draw conclusions on the overall efficiency of girdling on inducing rooting of olive cuttings.
Recommendations for Standardizing Thorax PET–CT in Non-Human Primates by Recent Experience from Macaque Studies
Authors: Stammes Marieke A. , Bakker Jaco , Vervenne Richard A. W. , Zijlmans Dian G. M. , van Geest Leo , Vierboom Michel P. M. , Langermans Jan A. M. , Verreck Frank A. W.
Published: January 2021

Despite the possibilities of routine clinical measures and assays on readily accessible bio-samples, it is not always essential in animals to investigate the dynamics of disease longitudinally. In this regard, minimally invasive imaging methods provide powerful tools in preclinical research. They can contribute to the ethical principle of gathering as much relevant information per animal as possible. Besides, with an obvious parallel to clinical diagnostic practice, such imaging platforms are potent and valuable instruments leading to a more refined use of animals from a welfare perspective. Non-human primates comprise highly relevant species for preclinical research to enhance our understanding of disease mechanisms and/or the development of improved prophylactic or therapeutic regimen for various human diseases. In this paper, we describe parameters that critically affect the quality of integrated positron emission tomography and computed tomography (PET–CT) in non-human primates. Lessons learned are exemplified by results from imaging experimental infectious respiratory disease in macaques; specifically tuberculosis, influenza, and SARS-CoV-2 infection. We focus on the thorax and use of 18F-fluorodeoxyglucose as a PET tracer. Recommendations are provided to guide various stages of PET–CT-supported research in non-human primates, from animal selection, scan preparation, and operation, to processing and analysis of imaging data.
Breathing Pattern Analysis in Cattle Using Infrared Thermography and Computer Vision
Authors: Kim Sueun , Hidaka Yuichi
Published: January 2021

Breathing patterns can be considered a vital sign providing health information. Infrared thermography is used to evaluate breathing patterns because it is non-invasive. Our study used not only sequence temperature data but also RGB images to gain breathing patterns in cattle. Mask R-CNN was used to detect the ROI (region of interest, nose) in the cattle RGB images. Mask segmentation from the ROI detection was applied to the corresponding temperature data. Finally, to visualize the breathing pattern, we calculated the temperature values in the ROI by averaging all temperature values in the ROI. The results in this study show 76% accuracy with Mask R-CNN in detecting cattle noses. With respect to the temperature calculation methods, the averaging method showed the most appropriate breathing pattern compared to other methods (maximum temperature in the ROI and integrating all temperature values in the ROI). Finally, we compared the breathing pattern from the averaging method and that from the thermal image observation and found them to be highly correlated (R2 = 0.91). This method is not labor-intensive, can handle big data, and is accurate. In addition, we expect that the characteristics of the method might enable the analysis of temperature data from various angles.
Influence of Milk Thistle (Silybum marianum) Seed Cakes on Biochemical Values of Equine Plasma Subjected to Physical Exertion
Authors: Dockalova Hana , Zeman Ladislav , Horky Pavel
Published: January 2021

Veterinarians can recommend milk thistle for the treatment of equine liver disease and laminitis. Milk thistle seed cakes were fed in the range of normal feed doses in this study. The milk thistle seed cakes were fed (twice a day) to the experimental group of the horses (n = 5) and biochemical blood markers (TP, Albumin, ALT (alanine transaminase), AST (aspartate transaminase), ALP (alkaline phosphatase), GGT (gamma-glutamyltransferase), Bilirubin, Cholesterol, HDL (high-density lipoprotein), LDL (low-density lipoprotein), TAG (triacylglycerol), BHB (beta-hydroxybutyric acid), NEFA (non-esterified fatty acids), creatine kinase, creatinine, Urea, GSH-Px (glutathione peroxidase), TAS (total antioxidant status), lactate, glucose, cortisol, Ca, Pi) were monitored. The control group of horses (n = 5), bred and trained in the same conditions, was used for comparison. The control group received the entire feed dose as accepted by the horses in the experimental group before the beginning of the experiment. The aim was to find out whether the preparation of milk thistle seed cakes could have positive effects on the health of the horses. All ten horses received one feeding form before the beginning of the experimental monitoring. All horses were exposed to heavy physical exercise (regular combined driving training) after 56 days of milk thistle seed cakes feeding (up to 400 g/day). Three blood samples were taken (before physical exercise; about 15 min and 60 min after physical exercise). Significant differences (p < 0.05) were detected in the values of AST, NEFA, cortisol and Pi in the experimental group. The exercise effect was detected in the values of albumin, lactate, cortisol, NEFA, and calcium. Our results suggest that the feeding of milk thistle seed cakes could have a positive effect on the health of the horses.
Cover Crop Management on the Southern High Plains: Impacts on Crop Productivity and Soil Water Depletion
Authors: Baxter Lisa L. , West Charles P. , Brown C. Philip , Green Paul E.
Published: January 2021

The imminent depletion of the Ogallala Aquifer demands innovative cropping alternatives. Even though the benefits of cover crops are well recognized, adoption has been slow in the Southern High Plains (SHP) of the United States because of concerns that cover crops withdraw soil water to the detriment of the summer crops. This small plot experiment tested the interacting effects—production, soil water depletion of the cover crops, and subsequent teff [Eragrostis tef (Zucc.) Trotter] summer hay crops—of irrigation and tillage management with five cover crop types to identify low-risk cover crop practices in the drought-prone SHP. Dryland rye (Secale cereale L.) produced modest forage biomass (>1000 kg ha−1), even in a dry year, but it was found that light irrigation should be used to ensure adequate forage supply (>1200 kg ha−1) if winter grazing is desired. No-till management and timely termination of the winter cover crops were crucial to reducing the negative impact of winter crops on summer teff production. The results indicated no detriment to soil water content that was attributable to planting no-till cover crops compared with the conventional practice of winter fallow. Therefore, producers could take advantage of the soil-conserving attributes of high-quality winter forage cover crops without experiencing significant soil water depletion.