bluuebunny/crossref_metadata_2025 huggingface dataset:
Intestinal Permeability and Dysbiosis in Female Patients with Recurrent Cystitis: A Pilot Study
Authors: Graziani Cristina , Laterza Lucrezia , Talocco Claudia , Pizzoferrato Marco , Di Simone Nicoletta , D’Ippolito Silvia , Ricci Caterina , Gervasoni Jacopo , Persichilli Silvia , Del Chierico Federica , Marzano Valeria , Mortera Stefano Levi , Primiano Aniello , Poscia Andrea , Ponziani Francesca Romana , Putignani Lorenza , Urbani Andrea , Petito Valentina , Di Vincenzo Federica , Masi Letizia , Lopetuso Loris Riccardo , Cammarota Giovanni , Romualdi Daniela , Lanzone Antonio , Gasbarrini Antonio , Scaldaferri Franco
Published: June 2022

Recurrent cystitis (RC) is a common disease, especially in females. Anatomical, behavioral and genetic predisposing factors are associated with the ascending retrograde route, which often causes bladder infections. RC seems to be mainly caused by agents derived from the intestinal microbiota, and most frequently by Escherichia coli. Intestinal contiguity contributes to the etiopathogenesis of RC and an alteration in intestinal permeability could have a major role in RC. The aim of this pilot study is to assess gut microbiome dysbiosis and intestinal permeability in female patients with RC. Patients with RC (n = 16) were enrolled and compared with healthy female subjects (n = 15) and patients with chronic gastrointestinal (GI) disorders (n = 238). We calculated the Acute Cystitis Symptom Score/Urinary Tract Infection Symptom Assessment (ACSS/UTISA) and Gastrointestinal Symptom Rating Scale (GSRS) scores and evaluated intestinal permeability and the fecal microbiome in the first two cohorts. Patients with RC showed an increased prevalence of gastrointestinal symptoms compared with healthy controls. Of the patients with RC, 88% showed an increased intestinal permeability with reduced biodiversity of gut microbiota compared to healthy controls, and 68% of the RC patients had a final diagnosis of gastrointestinal disease. Similarly, GI patients reported a higher incidence of urinary symptoms with a diagnosis of RC in 20%. Gut barrier impairment seems to play a major role in the pathogenesis of RC. Further studies are necessary to elucidate the role of microbiota and intestinal permeability in urinary tract infections.
Regulation of Key Immune-Related Genes in the Heart Following Burn Injury
Authors: Wen Jake J. , Mobli Keyan , Radhakrishnan Geetha L. , Radhakrishnan Ravi S.
Published: June 2022

Immune cascade is one of major factors leading to cardiac dysfunction after burn injury. TLRs are a class of pattern-recognition receptors (PRRs) that initiate the innate immune response by sensing conserved molecular patterns for early immune recognition of a pathogen. The Rat Toll-Like Receptor (TLR) Signaling Pathway RT² Profiler PCR Array profiles the expression of 84 genes central to TLR-mediated signal transduction and innate immunity, and is a validated tool for identifying differentially expressed genes (DEGs). We employed the PCR array to identify burn-induced cardiac TLR-signaling-related DEGs. A total of 38 up-regulated DEGs and 19 down-regulated DEGs were identified. Network analysis determined that all DEGS had 10 clusters, while up-regulated DEGs had 6 clusters and down-regulated DEGs had 5 clusters. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that DEGs were involved in TLR signaling, the RIG-I-Like receptor signaling pathway, the IL-17 signaling pathway, and the NFkB signaling pathway. Function analysis indicated that DEGs were associated with Toll-like receptor 2 binding, Lipopeptide binding, Toll-like receptor binding, and NAD(P)+ nucleosidase activity. The validation of 18 up-regulated DEGs (≥10-fold change) and 6 down-regulated DEGs (≤5-fold change) demonstrated that the PCR array is a trusted method for identifying DEGs. The analysis of validated DEG-derived protein–protein interaction networks will guide our future investigations. In summary, this study not only identified the TLR-signaling-pathway-related DEGs after burn injury, but also confirmed that the burn-induced cardiac cytokine cascade plays an important role in burn-induced heart dysfunction. The results will provide the novel therapeutic targets to protect the heart after burn injury.
Role of ABCA1 in Cardiovascular Disease
Authors: Wang Jing , Xiao Qianqian , Wang Luyun , Wang Yan , Wang Daowen , Ding Hu
Published: June 2022

Cholesterol homeostasis plays a significant role in cardiovascular disease. Previous studies have indicated that ATP-binding cassette transporter A1 (ABCA1) is one of the most important proteins that maintains cholesterol homeostasis. ABCA1 mediates nascent high-density lipoprotein biogenesis. Upon binding with apolipoprotein A-I, ABCA1 facilitates the efflux of excess intracellular cholesterol and phospholipids and controls the rate-limiting step of reverse cholesterol transport. In addition, ABCA1 interacts with the apolipoprotein receptor and suppresses inflammation through a series of signaling pathways. Thus, ABCA1 may prevent cardiovascular disease by inhibiting inflammation and maintaining lipid homeostasis. Several studies have indicated that post-transcriptional modifications play a critical role in the regulation of ABCA1 transportation and plasma membrane localization, which affects its biological function. Meanwhile, carriers of the loss-of-function ABCA1 gene are often accompanied by decreased expression of ABCA1 and an increased risk of cardiovascular diseases. We summarized the ABCA1 transcription regulation mechanism, mutations, post-translational modifications, and their roles in the development of dyslipidemia, atherosclerosis, ischemia/reperfusion, myocardial infarction, and coronary heart disease.
Multifactorial Analysis of Endodontic Microsurgery Using Finite Element Models
Authors: Richert Raphael , Farges Jean-Christophe , Maurin Jean-Christophe , Molimard Jérôme , Boisse Philippe , Ducret Maxime
Published: June 2022

Background: The present study aimed to classify the relative contributions of four biomechanical factors—the root-end filling material, the apical preparation, the root resection length, and the bone height—on the root stresses of the resected premolar. Methods: A design of experiments approach based on a defined subset of factor combinations was conducted to calculate the influence of each factor and their interactions. Sixteen finite element models were created and analyzed using the von Mises stress criterion. The robustness of the design of experiments was evaluated with nine supplementary models. Results: The current study showed that the factors preparation and bone height had a high influence on root stresses. However, it also revealed that nearly half of the biomechanical impact was missed without considering interactions between factors, particularly between resection and preparation. Conclusions: Design of experiments appears to be a valuable strategy to classify the contributions of biomechanical factors related to endodontics. Imagining all possible interactions and their clinical impact is difficult and can require relying on one’s own experience. This study proposed a statistical method to quantify the mechanical risk when planning apicoectomy. A perspective could be to integrate the equation defined herein in future software to support decision-making.
Two Genetic Mechanisms in Two Siblings with Intellectual Disability, Autism Spectrum Disorder, and Psychosis
Authors: Huang Yu-Shu , Fang Ting-Hsuan , Kung Belle , Chen Chia-Hsiang
Published: June 2022

Intellectual disability (ID) and autism spectrum disorder (ASD) are complex neurodevelopmental disorders with high heritability. To search for the genetic deficits in two siblings affected with ID and ASD in a family, we first performed a genome-wide copy number variation (CNV) analysis using chromosomal microarray analysis (CMA). We found a 3.7 Mb microdeletion at 22q13.3 in the younger sister. This de novo microdeletion resulted in the haploinsufficiency of SHANK3 and several nearby genes involved in neurodevelopment disorders. Hence, she was diagnosed with Phelan–McDermid syndrome (PMS, OMIM#606232). We further performed whole-genome sequencing (WGS) analysis in this family. We did not detect pathogenic mutations with significant impacts on the phenotypes of the elder brother. Instead, we identified several rare, likely pathogenic variants in seven genes implicated in neurodevelopmental disorders: KLHL17, TDO2, TRRAP, EIF3F, ATP10A, DICER1, and CDH15. These variants were transmitted from his unaffected parents, indicating these variants have only moderate clinical effects. We propose that these variants worked together and led to the clinical phenotypes in the elder brother. We also suggest that the combination of multiple genes with moderate effects is part of the genetic mechanism of neurodevelopmental disorders.
Surgical Treatment for Empyema Thoracis: Prognostic Role of Preoperative Transthoracic Echocardiography and Serum Calcium
Authors: Chu Pei-Yi , Wu Yu-Cheng , Lin Ya-Ling , Chang Hung , Lee Shih-Chun , Huang Tsai-Wang , Tsai Yuan-Ming
Published: June 2022

Background: Empyema is a major cause of mortality and hospitalization. Symptoms include difficulty breathing and chest pain. Calcium plays an essential role in the physiology of the cardiovascular system. However, there is little evidence on the role of echocardiography and the serum calcium levels of patients undergoing video-assisted thoracoscopic surgery (VATS) for empyema. This study aimed to investigate the risk factors for postoperative mortality in patients with empyema who required surgery. Methods: This single-institution retrospective study compared the outcomes of VATS for thoracic empyema (in terms of survival and mortality) in 122 patients enrolled between July 2015 and June 2019. Results: This study examined patients with thoracic empyema. The majority of the patients were males (100/122, 81.9%). The in-hospital/30-day mortality rate was 10.6% (13 patients). The calcium levels were 7.82 ± 1.17 mg/dL in the survival group and 6.88 ± 1.88 mg/dL in the mortality group (p = 0.032). In the mortality group, the utilization of echocardiography and serum calcium levels independently contributed to the risk prediction more than clinical variables. Patients in our cohort exhibited elevated pulmonary artery systolic pressure (PASP) and hypocalcemia, which were associated with increased postoperative mortality. Conclusion: Elevated PASP and calcium levels at the low end of the normal range demonstrated significant prognostic value in predicting mortality in patients with thoracic empyema who required surgical intervention. Recognizing this potential is critical in order to obtain better outcomes.
HR-pQCT for the Evaluation of Muscle Quality and Intramuscular Fat Infiltration in Ageing Skeletal Muscle
Authors: Chow Simon Kwoon-Ho , van Mourik Marloes , Hung Vivian Wing-Yin , Zhang Ning , Li Michelle Meng-Chen , Wong Ronald Man-Yeung , Leung Kwok-Sui , Cheung Wing-Hoi
Published: June 2022

Myosteatosis is the infiltration of fat in skeletal muscle during the onset of sarcopenia. The quantification of intramuscular adipose tissue (IMAT) can be a feasible imaging modality for the clinical assessment of myosteatosis, important for the early identification of sarcopenia patients and timely intervention decisions. There is currently no standardized method or consensus for such an application. The aim of this study was to develop a method for the detection and analysis of IMAT in clinical HR-pQCT images of the distal tibia to evaluate skeletal muscle during the ageing process, validated with animal and clinical experimentation. A pre-clinical model of ovariectomized (OVX) rats with known intramuscular fat infiltration was used, where gastrocnemii were scanned by micro-computed tomography (micro-CT) at an 8.4 μm isotropic voxel size, and the images were analyzed using our modified IMAT analysis protocol. IMAT, muscle density (MD), and muscle volume (MV) were compared with SHAM controls validated with Oil-red-O (ORO) staining. Furthermore, the segmentation and IMAT evaluation method was applied to 30 human subjects at ages from 18 to 81 (mean = 47.3 ± 19.2). Muscle-related parameters were analyzed with functional outcomes. In the animal model, the micro-CT adipose tissue-related parameter of IMAT% segmented at −600 HU to 100 HU was shown to strongly associate with the ORO-positively stained area (r = 0.898, p = 0.002). For the human subjects, at an adjusted threshold of −600 to −20 HU, moderate positive correlations were found between MV and MD (r = 0.642, p < 0.001), and between MV and IMAT volume (r = 0.618, p < 0.01). Moderate negative correlations were detected between MD and IMAT% (r = −0.640, p < 0.001). Strong and moderate associations were found between age and MD (r = −0.763, p < 0.01), and age and IMAT (r = 0.559, p < 0.01). There was also a strong correlation between IMAT% and chair rise time (r = 0.671, p < 0.01). The proposed HR-pQCT evaluation protocol for intramuscular adipose-tissue produced MD and IMAT results that were associated with age and physical performance measures, and were of good predictive value for the progression of myosteatosis or sarcopenia. The protocol was also validated on animal skeletal muscle samples that showed a good representation of histological lipid content with positive correlations, further supporting the clinical application for the rapid evaluation of muscle quality and objective quantification of skeletal muscle at the peripheral for sarcopenia assessment.
Personalized Diagnosis and Therapy for Multiple Sclerosis
Authors: Ramo-Tello Cristina
Published: June 2022

This Special Issue, entitled “Personalized Diagnosis and Therapy for Multiple Sclerosis” encompasses eight publications that we consider relevant, because their reading will help the clinician working regularly with people who suffer from multiple sclerosis (MS) [...]
Correction: Ejaz et al. The Use of Soil Conditioners to Ensure a Sustainable Wheat Yield under Water Deficit Conditions by Enhancing the Physiological and Antioxidant Potentials. Land 2022, 11, 368
Authors: Ejaz Muhammad Kashif , Aurangzaib Muhammad , Iqbal Rashid , Shahzaman Muhammad , Habib-ur-Rahman Muhammad , El-Sharnouby Mohamed , Datta Rahul , Alzuaibr Fahad M. , Sakran Mohamed I. , Ogbaga Chukwuma C. , Sabagh Ayman EL
Published: June 2022

In the published publication [...]
The Forage Plantation Program between Desertification Mitigation and Livestock Feeding: An Economic Analysis
Authors: Belhouadjeb Fathi Abdellatif , Boumakhleb Abdallah , Toaiba Abdelhalim , Doghbage Abdelghafour , Habib Benbader , Boukerker Hassen , Murgueitio Enrique , Soufan Walid , Almadani Mohamad Isam , Daoudi Belkacem , Khadoumi Amar
Published: June 2022

To combat desertification and land degradation in arid and semi-arid zones, the government has adopted an approach of rehabilitation of pasturelands through forage plantations. This program was launched at the beginning of the 1990s and, to date, there has been no global or national study on the sustainability of the program, particularly its economic profitability. Our work’s principal objective was to perform an economic analysis of the forage plantation program across the different periods since the creation of the program until the year 2020, focusing on the economic evaluation parameters of the projects, such as mean annual net income, net present value, annuity of the net present value, profitability index and payback period, based on the data collected from the official institutions. The results showed that the forage plantation program significantly contributes to sustainable development in steppe areas.
Hydrological Cycle Performance at a Permeable Pavement Site and a Raingarden Site in a Subtropical Region
Authors: Chen Chi-Feng , Lin Jhe-Wei , Lin Jen-Yang
Published: June 2022

Low-impact development (LID) structures are widely used to mitigate urbanization impacts on hydrology. The performances of such structures are strongly affected by field conditions, such as the ratio of LID area to drainage area and rainfall properties, such as rainfall intensity. In this study, onsite continuous monitoring was performed at a permeable pavement site and a raingarden site in Taipei, Taiwan, to determine their water retention and groundwater recharge potential under subtropical weather. In addition, the verified Storm Water Management Model (SWMM) was used to illustrate the annual performance on the hydrological cycle. Based on one year of monitoring, data on 41 and 24 rainfall events were obtained at the permeable pavement and raingarden sites, respectively. The ratio of the permeable pavement area to the total drainage area was 36.0%, and this ratio was 15.9% for the raingarden. The results showed that the average runoff reduction rate was 14.7% at the permeable pavement site, and 98.3% of the rainfall was retained in the raingarden and an underground storage tank. The validated model showed that the permeable pavement site experienced 45.3% outflow, 31.6% evaporation, and 23.1% infiltration annually. For the raingarden with an underground storage tank, 91.4% of the annual rainfall infiltrated and was stored, with only 4.1% outflow. According to the observed rainfall event performance and the simulated annual performance, the permeable pavement and raingarden performed well in subtropical regions. Pavement that was approximately 1/3 permeable in a drainage area increased infiltration by approximately 20%, and a raingarden with a sufficient underground storage tank preserved over 90% of the rainfall.
The Digitization of Seniors: Analyzing the Multiple Confluence of Social and Spatial Divides
Authors: Arroyo-Menéndez Millán , Gutiérrez-Láiz Noelia , Criado-Quesada Blanca
Published: June 2022

The lower digitization among seniors must be understood in the context of the coming together of multiple digital divides. In addition to the obvious generation divide (age is one of the factors most determining digital uses), others also have an influence, such as a lower education or income level, which is characteristic of this group and also strongly correlated with lower use of new technologies. We also find gender differences in the digital uses of seniors (more pronounced than in the population as a whole) and a significant geospatial inequality in several variables. The latter is important due to both the rapid aging of the rural population, greater than that seen in the urban population, and the fact that the geographical areas with a lower income level, where the aging population tends to be concentrated to a greater extent, are also the areas where digitization reaches the least, in terms of both infrastructures and uses. This article addresses the multiconfluence of the aforementioned “digital divides in older people” (or “seniors”), trying to determine the effects and degree of importance of each, identify the main groups at risk of digital exclusion, and to characterize the technological uses of seniors and their main segments. To do this, we have used the microdata from the “Survey on Equipment and Use of Information and Communication Technologies in homes”, produced by the Spanish Statistical Office (INE) for the year 2020.
Suilyin Disrupts the Blood–Brain Barrier by Activating Group III Secretory Phospholipase A2
Authors: Sui Yutong , Chen Ying , Lv Qingyu , Zheng Yuling , Kong Decong , Jiang Hua , Huang Wenhua , Ren Yuhao , Liu Peng , Jiang Yongqiang
Published: June 2022

Serious diseases caused by Streptococcus suis serotype 2 (S. suis 2) include septicaemia and meningitis, which are associated with high morbidity and mortality. Proliferation in the blood can result in a breach of the blood–brain barrier (BBB) and provide entry into the cerebrospinal fluid (CSF), where bacteria cause inflammation of the meningeal membranes resulting in meningitis. The molecular mechanisms of how this pathogen crosses the BBB remain unclear. Suilysin (SLY) has been identified as an important secreted virulence factor of S. suis 2 and may play a vital role in provoking meningitis. In this investigation, we demonstrate that SLY can increase the paracellular permeability of BBB, both in vivo and in vitro, via the activation of group III secretory phospholipase A2 (PLA2G3). Our results indicate that at lower, sublytic concentrations, the toxin can stimulate cerebral microvascular endothelial cells to release TNF-α, thereby inducing high level expressions of PLA2G3. Abnormal elevations of PLA2G3 might further injure tissues through direct cytolytic effectors or other responses.
Effect of Malignancy on Semen Parameters
Authors: Shrem Guy , Azani Liat , Feferkorn Ido , Listovsky Tamar , Hussaini Sofia , Farber Benjamin , Dahan Michael H. , Salmon-Divon Mali
Published: June 2022

Purpose: We aimed to examine how various types of cancer, classified histologically, affect semen quality. Methods: The study group included 313 patients who were diagnosed with cancer and reached for a sperm cryopreservation before a gonadotoxic treatment (PG-Tx group). Their semen parameters were compared to those of two control groups: (a) individuals who attended a fertility investigation and were found to be above the limit of the lower reference value of the WHO 2010 manual (ARL group), and (b) fertile men, whose semen parameters were obtained from the dataset of the WHO 2020 manual. Results: Semen quality was significantly poorer in the PG-Tx group than in the ARL group. Differences included a 65.6% decrease in concentration, a 12.1% decrease in volume, a 72.7% decrease in total count, and a 33.0%, 22.2%, and 24.7% decrease in total motility, rapid motility, and progressive motility, respectively. Linear regression models comparing the PG-Tx and ARL groups revealed that the maximum reduction in total motility and concentration was in men with germ-cell tumors, whereas the minimum reduction was in hematological tumors. Similarly, all sperm quality parameters were significantly lower in the PG-Tx group than in the fertile-men group (p < 0.0001). Conclusions: While the effect of malignancy on semen parameters is debatable, we found that all examined types of cancer significantly impaired sperm quality parameters. Although the median of most semen parameters of patients with cancer were still in the normal WHO range, their fifth percentile, represents men with a delayed time to pregnancy.
A Closer Look at the Contact Conditions of a Block-on-Flat Wear Experiment
Authors: Rudnytskyj André , Larsson Roland , Gachot Carsten
Published: June 2022

Specific wear rates of tribosystems always rely on the data obtained from wear experiments. Nonetheless, the events taking place during an experiment may often lead to wide variations and low repeatability of the results. In this work, the authors attempt to take a closer look into the dynamic contact conditions of a dry linearly reciprocating block-on-flat wear experiment. The finite element method and Archard’s wear model are used through COMSOL Multiphysics® 5.2a and LiveLink™ for MATLAB® software to model the wear and study the influence of different conditions of the block surface and alignment of the sample. Changes of the geometry of the block and the contact pressure are quantified for several back and forth motions, using an extrapolation scheme in the wear modelling methodology. The tracking of such changes allow a dynamic overview of how the block contact area and the contact pressure distribution change throughout time. The results show how the assumption of a constant contact area and use of a nominal contact pressure in calculating the wear rate in such experiments can be inappropriate, especially in the presence of roughness and misalignments of the block.
Enhanced Energy Storage Performance of PVDF-Based Composites Using BN@PDA Sheets and Titania Nanosheets
Authors: Zhu Congcong , Yin Jinghua , Feng Yu , Li Jialong , Li Yanpeng , Zhao He , Yue Dong , Liu Xiaoxu
Published: June 2022

With the rapid development of modern electrical and electronic applications, the demand for high-performance film capacitors is becoming increasingly urgent. The energy density of a capacitor is dependent on permittivity and breakdown strength. However, the development of polymer-based composites with both high permittivity (εr) and breakdown strength (Eb) remains a huge challenge. In this work, a strategy of doping synergistic dual-fillers with complementary functionalities into polymer is demonstrated, by which high εr and Eb are obtained simultaneously. Small-sized titania nanosheets (STNSs) with high εr and high-insulating boron nitride sheets coated with polydopamine on the surface (BN@PDA) were introduced into poly(vinylidene fluoride) (PVDF) to prepare a ternary composite. Remarkably, a PVDF-based composite with 1 wt% BN@PDA and 0.5 wt% STNSs (1 wt% PVDF/BN@PDA−STNSs) shows an excellent energy storage performance, including a high εr of ~13.9 at 1 Hz, a superior Eb of ~440 kV/mm, and a high discharged energy density Ue of ~12.1 J/cm3. Moreover, the simulation results confirm that BN@PDA sheets improve breakdown strength and STNSs boost polarization, which is consistent with the experimental results. This contribution provides a new design paradigm for energy storage dielectrics.
Applying the Random Forest Method to Improve Burner Efficiency
Authors: Kovalnogov Vladislav , Fedorov Ruslan , Klyachkin Vladimir , Generalov Dmitry , Kuvayskova Yulia , Busygin Sergey
Published: June 2022

Fuel power plants are one of the main sources of pollutant emissions, so special attention should be paid to improving the efficiency of the fuel combustion process. The mathematical modeling of processes in the combustion chamber makes it possible to reliably predict and find the best dynamic characteristics of the operation of a power plant, in order to quantify the emission of harmful substances, as well as the environmental and technical and economic efficiency of various regime control actions and measures, and the use of new types of composite fuels. The main purpose of this article is to illustrate how machine learning methods can play an important role in modeling and predicting the performance and control of the combustion process. The paper proposes a mathematical model of an unsteady turbulent combustion process, presents a model of a combustion chamber with a combined burner, and performs a numerical study using the STAR-CCM+ multidisciplinary platform. The influence of various input indicators on the efficiency of burner devices, which is evaluated by several parameters at the output, is investigated. In this case, three possible states of the burners are assumed: optimal, satisfactory and unsatisfactory.
On Some Model Theoretic Properties of Totally Bounded Ultrametric Spaces
Authors: Sági Gábor , Al-Sabti Karrar
Published: June 2022

Continuing investigations initiated by the first author, we associate relational structures for metric spaces and investigate their model theoretic properties. In this paper, we consider ultrametric spaces. Among others, we show that any elementary substructure of the relational structure associated with a totally bounded ultrametric space X is dense in X. Further, we provide an explicit upper bound for a splitting chain of atomic types in ultrametric spaces of a finite spectrum. For ultrametric spaces, these results improve previous ones of the present authors and may have further practical applications in designing similarity detecting algorithms.
Global Prescribed-Time Stabilization of High-Order Nonlinear Systems with Asymmetric Actuator Dead-Zone
Authors: Guo Xin , Yao Hejun , Gao Fangzheng
Published: June 2022

This paper is concerned with the global prescribed-time stabilization problem for a class of uncertain high-order nonlinear systems (HONSs) with an asymmetric actuator dead-zone. Firstly, a new state-scaling transformation (SST) is developed for high-order nonlinear systems to change the original prescribed-time stabilization into the finite-time stabilization of the transformed one. The defects of the conventional one introduced in Song et al. (2017), which is unable to ensure the closed-loop stability behind a prespecified convergence time and a closed-loop system, which is only driven to the neighborhood of destination, is successfully overcome by introducing a switching mechanism in our proposed SST. Then, by using the adding a power integrator (API) technique, a state feedback controller is explicitly constructed to achieve the requirements of the closed-loop prescribed time convergence. Lastly, a liquid-level system is utilized to validate the theoretical results.
WAVECNV: A New Approach for Detecting Copy Number Variation by Wavelet Clustering
Authors: Guo Yang , Wang Shuzhen , Haque A. K. Alvi , Yuan Xiguo
Published: June 2022

Copy number variation (CNV) detection based on second-generation sequencing technology is the basis of much gene research, but the read depth is affected by mapping errors, repeated reads, and GC bias. The existing methods have low sensitivity to variation regions with a short length and small variation range. Therefore, it is necessary to improve the sensitivity of algorithms to short-variation fragments. This study proposes a new CNV-detection method named WAVECNV to solve this issue. The algorithm uses wavelet clustering to process the read depth and determine the normal cluster and abnormal cluster according to the size of the cluster. Then, according to the distance between genome bins and normal clusters, the outlier of each genome bin is evaluated. Finally, a statistical model is established, and the p-value test is used for calling CNVs. Through this method, the information of the short variation region is retained. WAVECNV was tested and compared with peer methods in terms of simulated data and real cancer-sequencing data. The results show that the sensitivity of WAVECNV is better than the existing methods. It also has high precision in data with low purity and coverage. In real data experiments, WAVECNV can detect more cancer genes than existing methods. Therefore, this method can be regarded as a conventional method in the field of genomic mutation analysis of cancer samples.
Dissolution-Driven Convection in a Porous Medium Due to Vertical Axis of Rotation and Magnetic Field
Authors: Reddy Gundlapally , Koteswararao Nilam , Ravi Ragoju , Paidipati Kiran , Chesneau Christophe
Published: June 2022

This article aims to study the effect of the vertical rotation and magnetic field on the dissolution-driven convection in a saturated porous layer with a first-order chemical reaction. The system’s physical parameters depend on the Vadasz number, the Hartmann number, the Taylor number, and the Damkohler number. We analyze them in an in-depth manner. On the other hand, based on an artificial neural network (ANN) technique, the Levenberg–Marquardt backpropagation algorithm is adopted to predict the distribution of the critical Rayleigh number and for the linear stability analysis. The simulated critical Rayleigh numbers obtained by the numerical study and the predicted critical Rayleigh numbers by the ANN are compared and are in good agreement. The system becomes more stable by increasing the Damkohler and Taylor numbers.
Advanced High-Strength Steels for Automotive Applications: Arc and Laser Welding Process, Properties, and Challenges
Authors: Perka Ashok Kumar , John Merbin , Kuruveri Udaya Bhat , Menezes Pradeep L.
Published: June 2022

In recent years, the demand for advanced high-strength steel (AHSS) has increased to improve the durability and service life of steel structures. The development of these steels involves innovative processing technologies and steel alloy design concepts. Joining these steels is predominantly conducted by following fusion welding techniques, such as gas metal arc welding, tungsten inert gas welding, and laser welding. These fusion welding techniques often lead to a loss of mechanical properties due to the weld thermal cycles in the heat-affected zone (HAZ) and the deposited filler wire chemistry. This review paper elucidates the current studies on the state-of-the-art of weldability on AHSS, with ultimate strength levels above 800 MPa. The effects of alloy designs on the HAZ softening, microstructure evolution, and the mechanical properties of the weld joints corresponding to different welding techniques and filler wire chemistry are discussed. More specifically, the fusion welding techniques used for the welding of AHSS were summarized. This review article gives an insight into the issues while selecting a particular fusion welding technique for the welding of AHSS.
Solvent Extraction of Metal Ions from Synthetic Copper Leaching Solution Using R4NCy
Authors: Sepúlveda Rossana , Toro Norman , Hernández Pía , Navarro Patricio , Vargas Cristian , Gálvez Edelmira , Castillo Jonathan
Published: June 2022

Recent works suggest that the use of ionic liquids in the copper solvent extraction industry is feasible. However, the reports did not use real solutions (or synthetic solutions with various elements). This fact remains poorly established, and the interaction efficiencies are still under study. The objective of this research is to explore the extraction and stripping of the four major elements present in a copper industrial pregnant leach solution (Cu(II), Fe(III), Mn(II), and Zn(II)) using the methyltrioctyl/decylammonium bis(2,4,4-trimethylpentyl)phosphinate (R4NCy) ionic liquid as an extractant. The work conditions studied in extraction were ionic liquid concentration, initial pH, and O/A ratio, and in stripping were H2SO4 concentration and O/A ratio. The test was carried out at room temperature and ambient pressure. High efficiency and selectivity (99.82% and 113,755 over Cu(II), respectively) were observed for Fe(III) extraction over the other elements. Moreover, after the extraction test, significant difficulty in stripping Fe(III) loaded in the ionic liquid was observed (28.7% at 0.5 M of H2SO4). Finally, the present study demonstrates that the R4NCy ionic liquid is not suitable for copper extraction because it has a higher selectivity for Fe(III) and Zn(II).
Influence of Basicity and Calcium-Containing Substances on the Consolidation Mechanism of Fluxed Iron Ore Pellets
Authors: Liu Kuo , Chen Feng , Guo Yufeng , Liu Yajing , Wang Shuai , Yang Lingzhi
Published: June 2022

The application of fluxed pellets in iron making industry has attracted considerable attention because of the better metallurgical properties than acid pellets and environmental friendliness compared to sinters. However, fluxed pellets with different binary basicity (CaO/SiO2) exhibited significant differences in phase composition, microstructure and consolidation mechanism. These differences mainly stemmed from the influence of calcium-containing substances in fluxed pellets. Herein, the theoretical investigation discovered the calcium-containing substances from fluxed pellets, including calcium iron silicate, calcium silicate and complex calcium ferrite (SFCA), which determined the properties of fluxed pellets. Microstructure analysis revealed that the calcium-containing substances filled between hematite particles were used as a binding phase to assist in pellets’ consolidation. Furthermore, the calcium-containing binding phase formed in the low-basicity (0.4–1.0) pellets was mainly composed of the calcium iron silicate glassy phase, while the binding phase of the high-basicity (1.0–1.2) pellets was dominated by SFCA belonging to SiO2-Fe2O3-CaO-Al2O3 multivariate system. In comparison, SFCA exhibited better crystallinity and reducibility than calcium iron silicate. Within the roasting temperature range of 1200–1250 °C, the increase of basicity contributed to the fluxed pellets obtaining better strength. To sum up, fluxed pellets with SFCA as the main calcium-containing binding phase can be obtained by increasing the basicity above 1.0–1.2, which was imperative for further improving the physical and metallurgical properties of fluxed pellets.
Micro/Nano Periodic Surface Structures and Performance of Stainless Steel Machined Using Femtosecond Lasers
Authors: Xu Xiaofeng , Cheng Laifei , Zhao Xiaojiao , Wang Jing , Chen Xinyi
Published: June 2022

The machining of micro/nano periodic surface structures using a femtosecond laser has been an academic frontier and hotspot in recent years. With an ultrahigh laser fluence and an ultrashort pulse duration, femtosecond laser machining shows unique advantages in material processing. It can process almost any material and can greatly improve the processing accuracy with a minimum machining size and heat-affected zone. Meanwhile, it can fabricate a variety of micro/nano periodic surface structures and then change a material’s surface performance dramatically, such as the material’s wetting performance, corrosive properties, friction properties, and optical properties, demonstrating great application potential in defense, medical, high-end manufacturing, and many other fields. In recent years, the research is gradually deepening from the basic theory to optimization design, intelligent control, and application technology. Nowadays, while focusing on metal structure materials, especially on stainless steel, research institutions in the field of micro and nano manufacturing have conducted systematic and in-depth experimental research using different experimental environments and laser-processing parameters. They have prepared various surface structures with different morphologies and periods with sound performance, and are one step closer to many civilian engineering applications. This paper reviews the study of micro/nano periodic surface structures and the performance of stainless steel machined using a femtosecond laser, obtains the general evolution law of surface structure and performance with the femtosecond laser parameters, points out several key technical challenges for future study, and provides a useful reference for the engineering research and application of femtosecond laser micro/nano processing technology.
A Comprehensive Review of Micro UAV Charging Techniques
Authors: Mohsan Syed Agha Hassnain , Othman Nawaf Qasem Hamood , Khan Muhammad Asghar , Amjad Hussain , Żywiołek Justyna
Published: June 2022

The groundbreaking Unmanned Aerial Vehicles (UAVs) technology has gained significant attention from both academia and industrial experts due to several applications, such as military missions, power lines inspection, precision agriculture, remote sensing, delivery services, traffic monitoring and many more. UAVs are expected to become a mainstream delivery element by 2040 to address the ever-increasing demand for delivery services. Similarly, UAV-assisted monitoring approaches will automate the inspection process, lowering mission costs, increasing access to remote locations and saving time and energy. Despite the fact that unmanned aerial vehicles (UAVs) are gaining popularity in both military and civilian applications, they have a number of limitations and critical problems that must be addressed in order for missions to be effective. One of the most difficult and time-consuming tasks is charging UAVs. UAVs’ mission length and travel distance are constrained by their low battery endurance. There is a need to study multi-UAV charging systems to overcome battery capacity limitations, allowing UAVs to be used for a variety of services while saving time and human resources. Wired and Wireless Power Transfer (WPT) systems have emerged as viable options to successfully solve this difficulty. In the past, several research surveys have focused on crucial aspects of wireless UAV charging. In this review, we have also examined the most emerging charging techniques for UAVs such as laser power transfer (LPT), distributed laser charging (DLC), simultaneous wireless information and power transfer (SWIPT) and simultaneous light wave information and power transfer (SLIPT). The classification and types of UAVs, as well as various battery charging methods, are all discussed in this paper. We’ve also addressed a number of difficulties and solutions for safe operation. In the final section, we have briefly discussed future research directions.
Rational Design of Microfluidic Glaucoma Stent
Authors: Graf Thomas , Kancerevycius Gitanas , Jonušauskas Linas , Eberle Patric
Published: June 2022

Glaucoma is a common, irreparable eye disease associated with high intraocular pressure. One treatment option is implantation of a stent to lower the intraocular pressure. A systematic approach to develop a microchannel stent meshwork that drains aqueous humor from the anterior chamber of the eye into the subconjunctival space is presented. The stent has a large number of outlets within its mesh structure that open into the subconjunctiva. The development approach includes a flow resistance model of the stent. Local adaption of the stent’s tubular dimensions allows for adjustment of the flow resistance. In this way, an evenly distributed outflow into the subconjunctiva is achieved. We anticipate that microblebs will form at the stent outlets. Their size is crucial for drainage and control of intraocular pressure. An analytical model for bleb drainage is developed based on the porous properties of the subconjunctival tissue. Both models—the stent flow resistance model and the bleb drainage model—are verified by numerical simulation. The models and numerical simulation are used to predict intraocular pressure after surgery. They allow for a systematic and personalized design of microchannel stents. Stents designed in this way can stabilize the intraocular pressure between an upper and lower limit.
Comparison of DNA Extraction and Amplification Techniques for Use with Engorged Hard-Bodied Ticks
Authors: Reifenberger Gretchen C. , Thomas Bryce A. , Rhodes DeLacy V. L.
Published: June 2022

Tick-borne infections are a serious threat to humans, livestock, and companion animals in many parts of the world, often leading to high morbidity and mortality rates, along with decreased production values and/or costly treatments. The prevalence of the microbes responsible for these infections is typically assessed by the molecular identification of pathogens within the tick vectors. Ticks sampled from animals are often engorged with animal blood, presenting difficulties in the amplification of nucleic acids due to the inhibitory effects of mammalian blood on the enzymes used in polymerase chain reactions (PCRs). This study tested two tick preparation methods, three methods of DNA extraction, and four commercially available DNA polymerases to determine the most reliable method of extracting and amplifying DNA from engorged ticks. Our study found that the phenol–chloroform extraction method yielded the highest concentration of DNA, yet DNA extracted by this method was amplified the least successfully. Thermo Scientific’s Phusion Plus PCR Master Mix was the best at amplifying the tick 16s rRNA gene, regardless of extraction method. Finally, our study identified that using the Qiagen DNeasy Blood & Tissues kit for DNA extraction coupled with either Phusion Plus PCR Master Mix or GoTaq DNA polymerase Master Mix is the best combination for the optimized amplification of DNA extracted from engorged ticks.
Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects
Authors: De Giani Alessandra , Sandionigi Anna , Zampolli Jessica , Michelotti Angela , Tursi Francesco , Labra Massimo , Di Gennaro Patrizia
Published: June 2022

The gut microbiota is implicated in diverse interactions affecting human health. The present study reports a randomized, double-blind, placebo-controlled clinical study conducted by administering a new synbiotic formulation composed of two Lactobacillus strains (L. plantarum and L. acidophilus) and one Bifidobacterium strain (B. animalis subsp. lactis) and two types of fructans (fructo-oligosaccharides with a degree of polymerization of 3–5 and inulin-type fructans with 10 DP). The effects of this synbiotic were evaluated on healthy subjects for 28 days and the maintenance of its efficacy was evaluated at the end of a follow-up period of 28 days. The synbiotic treatment contributes to higher biodiversity of the gut microbiota, increasing the community richness with respect to the group with the prebiotics alone and the placebo group. Its positive effect is also reflected in the variation of microbial community structure favoring the beneficial short-chain fatty acids bacterial producers. The amelioration of the health status of the subjects was also established by the reduction of common infectious disease symptom incidence, the stimulation of the gut immune system showing a noteworthy variation of fecal β-defensin2 and calprotectin levels, and the modulation of the response of the respiratory tract’s immune system by salivary IgA as well as total antioxidant capacity biomarkers.
Geoenvironmental Model for Roll-Type Uranium Deposits in the Texas Gulf Coast
Authors: Walton-Day Katherine , Blake Johanna , Seal Robert , Gallegos Tanya , Dupree Jean , Becher Kent
Published: June 2022

Geoenvironmental models were formulated by the U.S. Geological Survey in the 1990s to describe potential environmental effects of extracting different types of ore deposits in different geologic and climatic regions. This paper presents a geoenvironmental model for roll-front (roll-type) uranium deposits in the Texas Coastal Plain. The model reviews descriptive and quantitative information derived from environmental studies and existing databases to depict existing conditions and potential environmental concerns associated with mining this deposit type. This geoenvironmental model describes how features of the deposits including host rock; ore and gangue mineralogy; geologic, hydrologic, and climatic settings; and mining methods (legacy open-pit and in situ recovery [ISR]) influence potential environmental effects from mining. Element concentrations in soil and water are compared to regulatory thresholds to depict ambient surface water and groundwater conditions. Although most open-pit operations in this region have been reclaimed, concerns remain about groundwater quality at three of the four former mills that supported former open-pit mines and are undergoing closure activities. The primary environmental concerns with ISR mining are (1) radon gas at active ISR operations, (2) radiation or contaminant leakage during production and transport of ISR resin or yellowcake, (3) uranium excursions into groundwater surrounding active ISR operations, and (4) contamination of groundwater after ISR mining. Although existing regulations attempt to address these concerns, some problems remain. Researchers suggest that reactive transport modeling and a better understanding of geology, stratigraphy, and geochemistry of ISR production areas could minimize excursions into surrounding aquifers and improve results of groundwater restoration.