bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Tenascin C in Cardiac Reverse Remodeling Following Banding–Debanding of the Ascending Aorta
Authors: Perera-Gonzalez Mireia , Kiss Attila , Kaiser Philipp , Holzweber Michael , Nagel Felix , Watzinger Simon , Acar Eylem , Szabo Petra Lujza , Gonçalves Inês Fonseca , Weber Lukas , Pilz Patrick Michael , Budinsky Lubos , Helbich Thomas , Podesser Bruno Karl
Published: February 2021

Background: Tenascin-C (TN-C) plays a maladaptive role in left ventricular (LV) hypertrophy following pressure overload. However, the role of TN-C in LV regression following mechanical unloading is unknown. Methods: LV hypertrophy was induced by transverse aortic constriction for 10 weeks followed by debanding for 2 weeks in wild type (Wt) and TN-C knockout (TN-C KO) mice. Cardiac function was assessed by serial magnetic resonance imaging. The expression of fibrotic markers and drivers (angiotensin-converting enzyme-1, ACE-1) was determined in LV tissue as well as human cardiac fibroblasts (HCFs) after TN-C treatment. Results: Chronic pressure overload resulted in a significant decline in cardiac function associated with LV dilation as well as upregulation of TN-C, collagen 1 (Col 1), and ACE-1 in Wt as compared to TN-C KO mice. Reverse remodeling in Wt mice partially improved cardiac function and fibrotic marker expression; however, TN-C protein expression remained unchanged. In HCF, TN-C strongly induced the upregulation of ACE 1 and Col 1. Conclusions: Pressure overload, when lasting long enough to induce HF, has less potential for reverse remodeling in mice. This may be due to significant upregulation of TN-C expression, which stimulates ACE 1, Col 1, and alpha-smooth muscle actin (α-SMA) upregulation in fibroblasts. Consequently, addressing TN-C in LV hypertrophy might open a new window for future therapeutics.
Extensive Histopathological Characterization of Inflamed Bowel in the Dextran Sulfate Sodium Mouse Model with Emphasis on Clinically Relevant Biomarkers and Targets for Drug Development
Authors: Bonfiglio Rita , Galli Filippo , Varani Michela , Scimeca Manuel , Borri Filippo , Fazi Sara , Cicconi Rosella , Mattei Maurizio , Campagna Giuseppe , Schönberger Tanja , Raymond Ernest , Wunder Andreas , Signore Alberto , Bonanno Elena
Published: February 2021

This study aims to develop a reliable and reproducible inflammatory bowel disease (IBD) murine model based on a careful spatial–temporal histological characterization. Secondary aims included extensive preclinical studies focused on the in situ expression of clinically relevant biomarkers and targets involved in IBD. C57BL/6 female mice were used to establish the IBD model. Colitis was induced by the oral administration of 2% Dextran Sulfate Sodium (DSS) for 5 days, followed by 2, 4 or 9 days of water. Histological analysis was performed by sectioning the whole colon into rings of 5 mm each. Immunohistochemical analyses were performed for molecular targets of interest for monitoring disease activity, treatment response and predicting outcome. Data reported here allowed us to develop an original scoring method useful as a tool for the histological assessment of preclinical models of DSS-induced IBD. Immunohistochemical data showed a significant increase in TNF-α, α4β7, VEGFRII, GR-1, CD25, CD3 and IL-12p40 expression in DSS mice if compared to controls. No difference was observed for IL-17, IL-23R, IL-36R or F480. Knowledge of the spatial–temporal pattern distribution of the pathological lesions of a well-characterized disease model lays the foundation for the study of the tissue expression of meaningful predictive biomarkers, thereby improving translational success rates of preclinical studies for a personalized management of IBD patients.
The Role of Butyrylcholinesterase and Iron in the Regulation of Cholinergic Network and Cognitive Dysfunction in Alzheimer’s Disease Pathogenesis
Authors: Jasiecki Jacek , Targońska Monika , Wasąg Bartosz
Published: February 2021

Alzheimer’s disease (AD), the most common form of dementia in elderly individuals, is marked by progressive neuron loss. Despite more than 100 years of research on AD, there is still no treatment to cure or prevent the disease. High levels of amyloid-β (Aβ) plaques and neurofibrillary tangles (NFTs) in the brain are neuropathological hallmarks of AD. However, based on postmortem analyses, up to 44% of individuals have been shown to have high Aβ deposits with no clinical signs, due to having a “cognitive reserve”. The biochemical mechanism explaining the prevention of cognitive impairment in the presence of Aβ plaques is still unknown. It seems that in addition to protein aggregation, neuroinflammatory changes associated with aging are present in AD brains that are correlated with a higher level of brain iron and oxidative stress. It has been shown that iron accumulates around amyloid plaques in AD mouse models and postmortem brain tissues of AD patients. Iron is required for essential brain functions, including oxidative metabolism, myelination, and neurotransmitter synthesis. However, an imbalance in brain iron homeostasis caused by aging underlies many neurodegenerative diseases. It has been proposed that high iron levels trigger an avalanche of events that push the progress of the disease, accelerating cognitive decline. Patients with increased amyloid plaques and iron are highly likely to develop dementia. Our observations indicate that the butyrylcholinesterase (BChE) level seems to be iron-dependent, and reports show that BChE produced by reactive astrocytes can make cognitive functions worse by accelerating the decay of acetylcholine in aging brains. Why, even when there is a genetic risk, do symptoms of the disease appear after many years? Here, we discuss the relationship between genetic factors, age-dependent iron tissue accumulation, and inflammation, focusing on AD.
Accumulation of the Auxin Precursor Indole-3-Acetamide Curtails Growth through the Repression of Ribosome-Biogenesis and Development-Related Transcriptional Networks
Authors: Sánchez-Parra Beatriz , Pérez-Alonso Marta-Marina , Ortiz-García Paloma , Moya-Cuevas José , Hentrich Mathias , Pollmann Stephan
Published: February 2021

The major auxin, indole-3-acetic acid (IAA), is associated with a plethora of growth and developmental processes including embryo development, expansion growth, cambial activity, and the induction of lateral root growth. Accumulation of the auxin precursor indole-3-acetamide (IAM) induces stress related processes by stimulating abscisic acid (ABA) biosynthesis. How IAM signaling is controlled is, at present, unclear. Here, we characterize the ami1rooty double mutant, that we initially generated to study the metabolic and phenotypic consequences of a simultaneous genetic blockade of the indole glucosinolate and IAM pathways in Arabidopsisthaliana. Our mass spectrometric analyses of the mutant revealed that the combination of the two mutations is not sufficient to fully prevent the conversion of IAM to IAA. The detected strong accumulation of IAM was, however, recognized to substantially impair seed development. We further show by genome-wide expression studies that the double mutant is broadly affected in its translational capacity, and that a small number of plant growth regulating transcriptional circuits are repressed by the high IAM content in the seed. In accordance with the previously described growth reduction in response to elevated IAM levels, our data support the hypothesis that IAM is a growth repressing counterpart to IAA.
Assessment of Epinephrine and Norepinephrine in Gastric Carcinoma
Authors: Mehedințeanu Alina Maria , Sfredel Veronica , Stovicek Puiu Olivian , Schenker Michael , Târtea Georgică Costinel , Istrătoaie Octavian , Ciurea Ana-Maria , Vere Cristin Constantin
Published: February 2021

The aim of our study was to assess the sympathetic nervous system’s involvement in the evolution of gastric carcinoma in patients by analyzing the mediators of this system (epinephrine and norepinephrine), as well as by analyzing the histological expression of the norepinephrine transporter (NET). We conducted an observational study including 91 patients diagnosed with gastric carcinoma and an additional 200 patients without cancer between November 2017 and October 2018. We set the primary endpoint as mortality from any cause in the first two years after enrolment in the study. The patients were monitored by a 24-h Holter electrocardiogram (ECG) to assess sympathetic or parasympathetic predominance. Blood was also collected from the patients to measure plasma free metanephrine (Meta) and normetanephrine (N-Meta), and tumor histological samples were collected for the analysis of NET expression. All of this was performed prior to the application of any antineoplastic therapy. Each patient was monitored for two years. We found higher heart rates in patients with gastric carcinoma than those without cancer. Regarding Meta and N-Meta, elevated levels were recorded in the patients with gastric carcinoma, correlating with the degree of tumor differentiation and other negative prognostic factors such as tumor invasion, lymph node metastasis, and distant metastases. Elevated Meta and N-Meta was also associated with a poor survival rate. All these data suggest that the predominance of the sympathetic nervous system’s activity predicts increased gastric carcinoma severity.
Dysregulated Provision of Oxidisable Substrates to the Mitochondria in ME/CFS Lymphoblasts
Authors: Missailidis Daniel , Sanislav Oana , Allan Claire Y. , Smith Paige K. , Annesley Sarah J. , Fisher Paul R.
Published: February 2021

Although understanding of the biomedical basis of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is growing, the underlying pathological mechanisms remain uncertain. We recently reported a reduction in the proportion of basal oxygen consumption due to ATP synthesis by Complex V in ME/CFS patient-derived lymphoblast cell lines, suggesting mitochondrial respiratory inefficiency. This was accompanied by elevated respiratory capacity, elevated mammalian target of rapamycin complex 1 (mTORC1) signaling activity and elevated expression of enzymes involved in the TCA cycle, fatty acid β-oxidation and mitochondrial transport. These and other observations led us to hypothesise the dysregulation of pathways providing the mitochondria with oxidisable substrates. In our current study, we aimed to revisit this hypothesis by applying a combination of whole-cell transcriptomics, proteomics and energy stress signaling activity measures using subsets of up to 34 ME/CFS and 31 healthy control lymphoblast cell lines from our growing library. While levels of glycolytic enzymes were unchanged in accordance with our previous observations of unaltered glycolytic rates, the whole-cell proteomes of ME/CFS lymphoblasts contained elevated levels of enzymes involved in the TCA cycle (p = 1.03 × 10−4), the pentose phosphate pathway (p = 0.034, G6PD p = 5.5 × 10−4), mitochondrial fatty acid β-oxidation (p = 9.2 × 10−3), and degradation of amino acids including glutamine/glutamate (GLS p = 0.034, GLUD1 p = 0.048, GOT2 p = 0.026), branched-chain amino acids (BCKDHA p = 0.028, BCKDHB p = 0.031) and essential amino acids (FAH p = 0.036, GCDH p = 0.006). The activity of the major cellular energy stress sensor, AMPK, was elevated but the increase did not reach statistical significance. The results suggest that ME/CFS metabolism is dysregulated such that alternatives to glycolysis are more heavily utilised than in controls to provide the mitochondria with oxidisable substrates.
Effect of Oxaliplatin, Olaparib and LY294002 in Combination on Triple-Negative Breast Cancer Cells
Authors: Andreidesz Kitti , Koszegi Balazs , Kovacs Dominika , Bagone Vantus Viola , Gallyas Ferenc , Kovacs Krisztina
Published: February 2021

Triple-negative breast cancer (TNBC) has a poor prognosis as the therapy has several limitations, most importantly, treatment resistance. In this study we examined the different responses of triple-negative breast cancer line MDA-MB-231 and hormone receptor-positive breast cancer line MCF7 to a combined treatment including olaparib, a poly-(ADP ribose) polymerase (PARP) inhibitor, oxaliplatin, a third-generation platinum compound and LY294002, an Akt pathway inhibitor. We applied the drugs in a single, therapeutically relevant concentration individually and in all possible combinations, and we assessed the viability, type of cell death, reactive oxygen species production, cell-cycle phases, colony formation and invasive growth. In agreement with the literature, the MDA-MB-231 cells were more treatment resistant than the MCF7 cells. However, and in contrast with the findings of others, we detected no synergistic effect between olaparib and oxaliplatin, and we found that the Akt pathway inhibitor augmented the cytostatic properties of the platinum compound and/or prevented the cytoprotective effects of PARP inhibition. Our results suggest that, at therapeutically relevant concentrations, the cytotoxicity of the platinum compound dominated over that of the PARP inhibitor and the PI3K inhibitor, even though a regression-based model could have indicated an overall synergy at lower and/or higher concentrations.
Maternal Melatonin Deficiency Leads to Endocrine Pathologies in Children in Early Ontogenesis
Authors: Ivanov Dmitry O. , Evsyukova Inna I. , Mironova Ekaterina S. , Polyakova Victoria O. , Kvetnoy Igor M. , Nasyrov Ruslan A.
Published: February 2021

The review summarizes the results of experimental and clinical studies aimed at elucidating the causes and pathophysiological mechanisms of the development of endocrine pathology in children. The modern data on the role of epigenetic influences in the early ontogenesis of unfavorable factors that violate the patterns of the formation of regulatory mechanisms during periods of critical development of fetal organs and systems and contribute to the delayed development of pathological conditions are considered. The mechanisms of the participation of melatonin in the regulation of metabolic processes and the key role of maternal melatonin in the formation of the circadian system of regulation in the fetus and in the protection of the genetic program of its morphofunctional development during pregnancy complications are presented. Melatonin, by controlling DNA methylation and histone modification, prevents changes in gene expression that are directly related to the programming of endocrine pathology in offspring. Deficiency and absence of the circadian rhythm of maternal melatonin underlies violations of the genetic program for the development of hormonal and metabolic regulatory mechanisms of the functional systems of the child, which determines the programming and implementation of endocrine pathology in early ontogenesis, contributing to its development in later life. The significance of this factor in the pathophysiological mechanisms of endocrine disorders determines a new approach to risk assessment and timely prevention of offspring diseases even at the stage of family planning.
Heightened Crescentic Glomerulonephritis in Immune Challenged 129sv Mice Is TGF-β/Smad3 Dependent
Authors: Du Yong , Xie Chun , Ravikumar Sneha , Orme Jacob , Li Li , Zhou Xin J , Mohan Chandra
Published: February 2021

The 129sv mouse strain is particularly sensitive to experimental immune-mediated nephritis. Previous studies have indicated that transforming growth factor-β (TGF-β) plays a critical role in both immune modulation and tissue fibrogenesis in various diseases and that its biological activities are exerted via the SMAD family. In this study, we aimed to determine whether TGF-β/SMAD signaling is essential for the development of immune-mediated nephritis in 129sv mice. Relative to C57BL/6J control mice with anti-glomeruli basement membrane (GBM) nephritis, 129sv mice with anti-GBM nephritis exhibited increased renal collagen deposition. Additionally, higher mRNA levels of pro-collagen and collagen IV, higher serum levels of active and total TGF-β1, and increased TGF-β1, TGF-βIIR, and phosphorylated SMAD expression were detected in these mice. Deletion of Smad3 in 129sv mice ameliorated anti-GBM induced nephritis, including crescentic glomerulonephritis. Collectively, these findings indicate that the heightened experimental nephritis and fibrotic disease in the 129sv strain of mice are regulated by SMAD3, which could be a potential therapeutic target for immune-mediated nephritis.
Prognostic Role of Tumor Budding in Breast Cancer Patients Receiving Neo-Adjuvant Therapy
Authors: Mozarowski Paul , Rasaiah Bhubendra , Reed Melissa , Lewis Alexis , Walde Natalie , Voutsadakis Ioannis A.
Published: February 2021

Background: Isolated tumor cells or small clusters of tumor cells observed in the vicinity of the main tumor mass in pathology sections, termed tumor budding, are common in cancers and have been associated with prognosis in some settings. This study examined the clinical associations and treatment efficacy implications of tumor budding in breast cancer patients receiving neo-adjuvant therapy. Methods: Breast cancer patients that received neo-adjuvant therapy before definitive surgical treatment in a single cancer center over a 7-year period were included, and their records were reviewed. Data extracted included patient demographics, tumor characteristics and pathologic response to treatment at surgery. The initial breast cancer biopsy before any therapy was reviewed by two pathologists, and a hot spot area was evaluated for tumor budding (defined as 1 to 5 cancer cells observed detached from the main tumor mass). Results: Seventy-five patients who received neo-adjuvant therapy (73 received chemotherapy and 2 received hormonal therapy) were included. Tumor budding was observed in two-thirds of the patients. There were no significant differences in patient (age and menopause status) and tumor (stage, histology and molecular sub-type equivalent) characteristics between the group that had tumor budding and the group that did not have tumor budding in the pre-treatment biopsy. Likewise, no statistically significant differences were observed in the frequency of complete or partial responses between the two groups. Conclusion: In this cohort of breast cancer patients receiving neo-adjuvant therapy, tumor budding was frequent, but it was not associated with tumor characteristics or pathologic responses to treatment. The value of tumor budding as a prognostic factor in the neo-adjuvant setting within the general breast cancer population could not be confirmed, but such a value in specific sub-groups deserves further investigation, given the pathophysiologic rationale and data from other settings.
Risk of Insulin Resistance and Metabolic Syndrome in Women with Hyperandrogenemia: A Comparison between PCOS Phenotypes and Beyond
Authors: Borzan Valentin , Lerchbaum Elisabeth , Missbrenner Cornelia , Heijboer Annemieke C. , Goschnik Michaela , Trummer Christian , Theiler-Schwetz Verena , Haudum Christoph , Gumpold Roswitha , Schweighofer Natascha , Obermayer-Pietsch Barbara
Published: February 2021

Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in premenopausal women, with a wide spectrum of possible phenotypes, symptoms and sequelae according to the current clinical definition. However, there are women who do not fulfill at least two out of the three commonly used “Rotterdam criteria” and their risk of developing type 2 diabetes or obesity later in life is not defined. Therefore, we addressed this important gap by conducting a retrospective analysis based on 750 women with and without PCOS. We compared four different PCOS phenotypes according to the Rotterdam criteria with women who exhibit only one Rotterdam criterion and with healthy controls. Hormone and metabolic differences were assessed by analysis of variance (ANOVA) as well as logistic regression analysis. We found that hyperandrogenic women have per se a higher risk of developing insulin resistance compared to phenotypes without hyperandrogenism and healthy controls. In addition, hyperandrogenemia is associated with developing insulin resistance also in women with no other Rotterdam criterion. Our study encourages further diagnostic and therapeutic approaches for PCOS phenotypes in order to account for varying risks of developing metabolic diseases. Finally, women with hyperandrogenism as the only symptom should also be screened for insulin resistance to avoid later metabolic risks.
Clinical Follow-Up in Orofacial Clefts—Why Multidisciplinary Care Is the Key
Authors: Lethaus Bernd , Grau Elisabeth , Kloss-Brandstätter Anita , Brauer Luise , Zimmerer Rüdiger , Bartella Alexander K. , Hahnel Sebastian , Sander Anna K.
Published: February 2021

(1) Background: Although most clinicians involved in the treatment of cleft patients agree upon the major importance of interdisciplinary cooperation and many protocols and concepts have been discussed in the literature, there is little evidence of the relevance of continuous interdisciplinary care. We aimed to objectify the type and number of therapeutic decisions resulting from an annual multidisciplinary follow-up. (2) Methods: We retrospectively analyzed the data of all 1126 patients followed up in the weekly consultation hours for cleft patients at university clinics in Leipzig for the years 2005–2020. We assessed the clinical data of every patient and specifically evaluated the treatment decisions taken at different points in time by the participating experts of different specialties. (3) Results: In total, 3470 consultations were included in the evaluation, and in 70% of those, a therapeutic recommendation was given. Each specialty showed certain time frames with intense treatment demand, which partially overlapped. Nearly all therapy recommendations were statistically attached to a certain age (p < 0.001). (4) Conclusions: There is an exceptionally high need for the interdisciplinary assessment of patients with cleft formation. Some developmental phases are of particular importance with regard to regular follow-up and initiation of different treatment protocols. The therapy and checkup of cleft patients should be concentrated in specialized centers.
Healthcare Professionals’ Perspectives on the Cross-Sectoral Treatment Pathway for Women with Gestational Diabetes during and after Pregnancy—A Qualitative Study
Authors: Timm Anne , Nielsen Karoline Kragelund , Christensen Ulla , Maindal Helle Terkildsen
Published: February 2021

Gestational diabetes mellitus (GDM) increases the risk of adverse outcomes during and after pregnancy, including a long-term risk of type 2 diabetes. Women with GDM are treated by numerous healthcare professionals during pregnancy and describe a lack of preventive care after pregnancy. We aim to investigate healthcare professionals’ perspectives on the cross-sectoral treatment pathway for women with GDM—during and after pregnancy. A qualitative study was conducted using systematic text condensation. Nine healthcare professionals (two general practitioners, four midwives, two obstetricians and one diabetes nurse) were interviewed and eight health visitors participated in two focus group discussions., Three major themes emerged: (1) “professional identities”, which were identified across healthcare professionals and shaped care practices; (2) ”unclear guidelines on type 2 diabetes prevention after GDM”, which contributed to uncertainty about tasks and responsibilities during and after pregnancy; and (3) “cross-sectoral collaboration”, which relied heavily on knowledge transfers between hospitals, general practice and the local municipality. The findings implicate that clear, transparent guidelines for all sectors should be prioritized to strengthen cross-sectoral care to women with GDM during and after pregnancy. As a result, strong cross-sectoral care throughout the GDM care pathway may improve maternal health by supporting healthy behaviors, facilitate weight loss and reduce the risk of subsequent GDM and early onset diabetes.
Medio-Lateral and Flexion-Extension Gap Imbalances in Mechanically Aligned Total Knee Arthroplasty Using Measured Resection Technique in Korean Patients: 3D Simulation
Authors: Cho Byung Woo , Nam Ji Hoon , Koh Yong Gon , Park Kwan Kyu , Kang Kyoung Tak
Published: February 2021

Background: It is well known that the measured resection (MR) technique in mechanically aligned (MA) total knee arthroplasty (TKA) generates significant gap imbalances, but little is known about whether this applies to the knees of Asian patients. The aim of this study was to evaluate the medio-lateral and flexion-extension gap imbalances and to find the most optimal posterior femoral condyle resection method for operating on the knees of Asian patients. Methods: In total, 738 magnetic resonance imaging (MRI) scans of consecutive patients who underwent TKA were obtained. Four posterior femoral condylar resection methods were used: alignment by the surgical transepicondylar axis (TEA), Whiteside’s line (WSL), 3° external rotation to the posterior condylar axis (PCA), and flexion-extension axis (FEA). Results: For the medial compartments, there were significant differences between the flexion and extension gaps in the varus knee group in all four methods, but there were no differences between the flexion and extension gaps in the valgus knee group. For the lateral compartment, all the methods showed significant differences except for WSL of the valgus knee group and FEA of the varus knee group. Conclusions: In Asian patients, the use of the MA MR technique inevitably leads to medio-lateral or flexion-extension imbalances. Therefore, surgeons should consider which methods can minimize imbalances and choose the best method within the technically possible range.
The Effect of Ventilation with Individualized Positive End-Expiratory Pressure on Postoperative Atelectasis in Patients Undergoing Robot-Assisted Radical Prostatectomy: A Randomized Controlled Trial
Authors: Yoon Hyun-Kyu , Kim Bo Rim , Yoon Susie , Jeong Young Hyun , Ku Ja Hyeon , Kim Won Ho
Published: February 2021

For patients undergoing robot-assisted radical prostatectomy, the pneumoperitoneum with a steep Trendelenburg position could worsen intraoperative respiratory mechanics and result in postoperative atelectasis. We investigated the effects of individualized positive end-expiratory pressure (PEEP) on postoperative atelectasis, evaluated using lung ultrasonography. Sixty patients undergoing robot-assisted radical prostatectomy were randomly allocated into two groups. Individualized groups (n = 30) received individualized PEEP determined by a decremental PEEP trial using 20 to 7 cm H2O, aiming at maximizing respiratory compliance, whereas standardized groups (n = 30) received a standardized PEEP of 7 cm H2O during the pneumoperitoneum. Ultrasound examination was performed on 12 sections of thorax, and the lung ultrasound score was measured as 0–3 by considering the number of B lines and the degree of subpleural consolidation. The primary outcome was the difference between the lung ultrasound scores measured before anesthesia induction and just after extubation in the operating room. An increase in the difference means the development of atelectasis. The optimal PEEP in the individualized group was determined as the median (interquartile range) 14 (12–18) cm H2O. Compared with the standardized group, the difference in the lung ultrasound scores was significantly smaller in the individualized group (−0.5 ± 2.7 vs. 6.0 ± 2.9, mean difference −6.53, 95% confidence interval (−8.00 to −5.07), p < 0.001), which means that individualized PEEP was effective to reduce atelectasis. The lung ultrasound score measured after surgery was significantly lower in the individualized group than the standardized group (8.1 ± 5.7 vs. 12.2 ± 4.2, mean difference −4.13, 95% confidence interval (−6.74 to −1.53), p = 0.002). However, the arterial partial pressure of the oxygen/fraction of inspired oxygen levels during the surgery showed no significant time-group interaction between the two groups in repeated-measures analysis of variance (p = 0.145). The incidence of a composite of postoperative respiratory complications was comparable between the two groups. Individualized PEEP determined by maximal respiratory compliance during the pneumoperitoneum and steep Trendelenburg position significantly reduced postoperative atelectasis, as evaluated using lung ultrasonography. However, the clinical significance of this finding should be evaluated by a larger clinical trial.
The Accuracy of Emergency Physicians’ Suspicions of Active Pulmonary Tuberculosis
Authors: Chen Shiang-Jin , Lin Chun-Yu , Huang Tzu-Ling , Hsu Ying-Chi , Liu Kuan-Ting
Published: February 2021

Objective: To investigate factors associated with recognition and delayed isolation of pulmonary tuberculosis (PTB). Background: Precise identification of PTB in the emergency department (ED) remains challenging. Methods: Retrospectively reviewed PTB suspects admitted via the ED were divided into three groups based on the acid-fast bacilli culture report and whether they were isolated initially in the ED or general ward. Factors related to recognition and delayed isolation were statistically compared. Results: Only 24.94% (100/401) of PTB suspects were truly active PTB and 33.77% (51/151) of active PTB were unrecognized in the ED. Weight loss (p = 0.022), absence of dyspnea (p = 0.021), and left upper lobe field (p = 0.024) lesions on chest radiographs were related to truly active PTB. Malignancy (p = 0.015), chronic kidney disease (p = 0.047), absence of a history of PTB (p = 0.013), and lack of right upper lung (p ≤ 0.001) and left upper lung (p = 0.020) lesions were associated with PTB being missed in the ED. Conclusions: Weight loss, absence of dyspnea, and left upper lobe field lesions on chest radiographs were related to truly active PTB. Malignancy, chronic kidney disease, absence of a history of PTB, and absence of right and/or left upper lung lesions on chest radiography were associated with isolation delay.
The Relationship between Theory of Mind and Intelligence: A Formative g Approach
Authors: Navarro Ester , Goring Sara Anne , Conway Andrew R. A.
Published: February 2021

Theory of Mind (ToM) is the ability understand that other people’s mental states may be different from one’s own. Psychometric models have shown that individual differences in ToM can largely be attributed to general intelligence (g) (Coyle et al. 2018). Most psychometric models specify g as a reflective latent variable, which is interpreted as a general ability that plays a causal role in a broad range of cognitive tasks, including ToM tasks. However, an alternative approach is to specify g as a formative latent variable, that is, an overall index of cognitive ability that does not represent a psychological attribute (Kovacs and Conway 2016). Here we consider a formative g approach to the relationship between ToM and intelligence. First, we conducted an SEM with reflective g to test the hypothesis that ToM is largely accounted for by a general ability. Next, we conducted a model with formative g to determine whether the relationship between ToM and intelligence is influenced by domain-specific tasks. Finally, we conducted a redundancy analysis to examine the contribution of each g variable. Results suggest that the relationship between ToM and intelligence in this study was influenced by language-based tasks, rather than solely a general ability.
A Method to Detect Anomalies in Complex Socio-Technical Operations Using Structural Similarity
Authors: Danial Syed Nasir , Smith Doug , Veitch Brian
Published: February 2021

Traditional techniques for accident investigation have hindsight biases. Specifically, they isolate the process of the accident event and trace backward from the event to determine the factors leading to the accident. Nonetheless, the importance of the contributing factors towards a successful operation is not considered in conventional accident modeling. The Safety-II approach promotes an examination of successful operations as well as failures. The rationale is that there is an opportunity to learn from successful operations, in addition to failure, and there is an opportunity to further differentiate failure processes from successful operations. The functional resonance analysis method (FRAM) has the capacity to monitor the functionality and performance of a complex socio-technical system. The method can model many possible ways a system could function, then captures the specifics of the functionality of individual operational events in functional signatures. However, the method does not support quantitative analysis of the functional signatures, which may demonstrate similarities as well as differences among each other. This paper proposes a method to detect anomalies in operations using functional signatures. The present work proposes how FRAM data models can be converted to graphs and how such graphs can be used to estimate anomalies in the data. The proposed approach is applied to human performance data obtained from ice-management tasks performed by a cohort of cadets and experienced seafarers in a ship simulator. The results show that functional differences can be captured by the proposed approach even though the differences were undetected by usual statistical measures.
The Response of the Water Surface Layer to Internal Turbulence and Surface Forcing
Authors: Barzegar Mohammad , Bogucki Darek , Haus Brian K. , Shao Mingming
Published: February 2021

We have carried out an experimental study of the turbulence kinetic energy dissipation rate (ϵ), temperature dissipation rate (χ), and turbulent heat flux (THF) within the water surface layer in the presence of non-breaking wave, surface convection, and horizontal heat and eddy fluxes that play a prominent role in the front. We noted that the non-breaking wave dominates ϵ values within the surface layer. While analyzing the vertical ϵ variability, the presence of a wave-affected layer from the water surface to a depth of z≈1.25λw is observed, where λw is the wavelength. ϵ associated with non-breaking waves ranged to 4.9×10−6–7×10−6 m2/s3 for the wavelength range of 0.038 m < λw < 0.098 m categorized as the gravity and gravity-capillary wave regimes. ϵ values increase for longer λw and non-breaking wave ϵ values represent their significant contribution to the ocean energy budget and dynamic of surface layer considering that the non-breaking wave covers the large fraction of ocean surface. We also found that the surface mean square slope (MSS) and wave generated ϵ have the same order of magnitude, i.e., MSS ∼ϵ. Besides, we have documented that the small-scale temperature fluctuation change (i.e., χ) is consistent with the large-scale temperature gradient change (i.e., d/dz). The value of the THF is approximately constant within the surface layer. It represents that the measured THF near the water surface can be considered a surface water THF, challenging to measure directly.
Evolution Trend Research of Global Ocean Power Generation Based on a 45-Year Scientometric Analysis
Authors: Chen Lisu , Li Wei , Li Jie , Fu Qiang , Wang Tianzhen
Published: February 2021

Since 1973, studies have explored ocean power generation from different perspectives. However, in the past 45 years, few studies have attempted to comprehensively review the existing studies on ocean power generation using wave energy, tidal current energy, ocean thermal energy, salinity gradient energy, bio-mass energy, and gas hydrates. In this study, we collected 5262 studies published from 1973 to 2018 for scientometric visualization analysis and drew a knowledge map of the ocean power generation field. The results show that the most important contributions to the research of ocean power generation mainly came from the United States, China, Britain, Italy, Spain, Japan, Norway, Germany, France, and Denmark. Ocean power generation research is mainly divided into two stages. From 1973 to 2007, there were relatively few studies and no obvious hot topics. From 2008 to 2018, the knowledge fields mainly focused on ocean biomass power generation, the exploitation of natural gas hydrates, the utilization of wave energy and tidal energy, the research and optimization of energy generators, the storage and management of ocean energy, and numerical simulations of marine climates. In addition, the joint utilization of wind energy and wave energy is also a current research topic of interest, including joint assessment of the two energy potentials, the research and development of equipment, and numerical simulations of joint power generation projects.
Comparison of the Economic Performances of Three Sulphur Oxides Emissions Abatement Solutions for a Very Large Crude Carrier (VLCC)
Authors: Fan Hongjun , Tu Huan , Enshaei Hossein , Xu Xiangyang , Wei Ying
Published: February 2021

Ship-source air pollutants, especially sulphur oxides (SOx), have a major impact on human health, the marine environment and the natural resources. Therefore, control of SOx emissions has become a main concern in the maritime industry. The International Maritime Organization (IMO) has set a global limit on sulphur content of 0.50% m/m (mass by mass) in marine fuels which has entered into effect on 1 January 2020.To comply with the sulphur limits, ship owners are facing the need to select suitable abatement solutions. The choice of a suitable solution is a compromise among many issues, but the economic performance offers the basis for which ones are attractive to ship owners. Currently, there are three technologically feasible SOx abatement solutions that could be used by ships, namely, liquified natural gas (LNG) as a fuel (Solution A), scrubbers (Solution B) and low-sulphur fuel oil (LSFO) (Solution C). To compare the economic performances of the mentioned three solutions for a newbuilding very large crude carrier (VLCC), this paper proposes a voyage expenses-based method (VEM). It was found that, within the initial target payback period of 6 years, Solution A and C are more expensive than Solution B, while Solution C is more competitive than Solution A. Five scenarios of target payback years were assumed to compare the trends of the three proposed solutions. The results show that Solution B maintains its comparative advantage. As the assumed target payback years becomes longer, the economy of Solution A gradually improves and the economics of Solution B and C gradually decline. A comparison between Solution A and C shows 6.5 years is a turning point. The advantage of Solution A is prominent after this payback period. In addition, the performance of a certain solution in terms of adaptability to the IMO greenhouse gas (GHG) emissions regulations is also a factor that ship owner need to consider when making decisions. In conclusion, when the IMO air pollutant regulations and GHG regulations are considered simultaneously, the advantages of using LNG are obvious.
Comparison of Plant Metabolites in Root Exudates of Lolium perenne Infected with Different Strains of the Fungal Endophyte Epichloë festucae var. lolii
Authors: Patchett Aurora , Newman Jonathan A.
Published: February 2021

Lolium perenne infected with the fungal endophyte Epichloë festucae var. lolii have specific, endophyte strain-dependent, chemical phenotypes in their above-ground tissues. Differences in these chemical phenotypes have been largely associated with classes of fungal-derived alkaloids which protect the plant against many insect pests. However, the use of new methodologies, such as various omic techniques, has demonstrated that many other chemical changes occur in both primary and secondary metabolites. Few studies have investigated changes in plant metabolites exiting the plant in the form of root exudates. As root exudates play an essential role in the acquisition of nutrients, microbial associations, and defense in the below-ground environment, it is of interest to understand how plant root exudate chemistry is influenced by the presence of strains of a fungal endophyte. In this study, we tested the influence of four strains of E. festucae var. lolii (E+ (also known as Lp19), AR1, AR37, NEA2), and uninfected controls (E−), on L. perenne growth and the composition of root exudate metabolites. Root exudates present in the hydroponic water were assessed by untargeted metabolomics using Accurate-Mass Quadrupole Time-of-Flight (Q–TOF) liquid chromatography–mass spectrometry (LC–MS). The NEA2 endophyte strain resulted in the greatest plant biomass and the lowest endophyte concentration. We found 84 metabolites that were differentially expressed in at least one of the endophyte treatments compared to E− plants. Two compounds were strongly associated with one endophyte treatment, one in AR37 (m/z 135.0546 RT 1.17), and one in E+ (m/z 517.1987 RT 9.26). These results provide evidence for important changes in L. perenne physiology in the presence of different fungal endophyte strains. Further research should aim to connect changes in root exudate chemical composition with soil ecosystem processes.
Transcriptome Profiles of Sporisorium reilianum during the Early Infection of Resistant and Susceptible Maize Isogenic Lines
Authors: Zhang Boqi , Zhang Nan , Zhang Qianqian , Xu Qianya , Zhong Tao , Zhang Kaiyue , Xu Mingliang
Published: February 2021

The biotrophic fungus Sporisorium reilianum causes destructive head smut disease in maize (Zea mays L.). To explore the pathogenicity arsenal of this fungus, we tracked its transcriptome changes during infection of the maize seedling mesocotyls of two near-isogenic lines, HZ4 and HZ4R, differing solely in the disease resistance gene ZmWAK. Parasitic growth of S. reilianum resulted in thousands of differentially expressed genes (DEGs) compared with growth in axenic culture. The protein synthesis and energy metabolism of S. reilianum were predominantly enriched with down-regulated DEGs, consistent with the arrested hyphal growth observed following colonization. Nutrition-related metabolic processes were enriched with both up- and down-regulated DEGs, which, together with activated transmembrane transport, reflected a potential transition in nutrition uptake of S. reilianum once it invaded maize. Notably, genes encoding secreted proteins of S. reilianum were mostly up-regulated during biotrophy. ZmWAK-mediated resistance to head smut disease reduced the number of DEGs of S. reilianum, particularly those related to the secretome. These observations deepen our understanding of the mechanisms underlying S. reilianum pathogenicity and ZmWAK-induced innate immunity.
The Integration of New-Type Urbanization and Rural Revitalization Strategies in China: Origin, Reality and Future Trends
Authors: Chen Mingxing , Zhou Yuan , Huang Xinrong , Ye Chao
Published: February 2021

New-type urbanization and rural revitalization have gradually become national strategies, and are an objective requirement for China to be able to enter into a new era of socialism with Chinese characteristics and also an inevitable result of the integration of new-type urbanization and rural development in the new stage. This paper reviews the classic theories and cognition of the research on urban–rural relations at home and abroad, and outlines the stage evolution characteristics of urban–rural relations in China. It is believed that urban-biased urbanization has widened the development gap between urban and rural areas since reform and opening up. Under the guidance of the two strategies of new-type urbanization and rural revitalization, urban and rural areas have transitioned from “one-way flow” to “bilateral interaction”, and from “urban bias” to “urban–rural integration”. This paper puts forward a research framework and scientific issues regarding the integration of new-type urbanization and rural revitalization from multidisciplinary perspectives. The integration of these two major strategies will contribute to a new situation of the coordinated and high-quality development of urban and rural areas in the new era.
Microalgae n-3 PUFAs Production and Use in Food and Feed Industries
Authors: Remize Marine , Brunel Yves , Silva Joana L. , Berthon Jean-Yves , Filaire Edith
Published: February 2021

N-3 polyunsaturated fatty acids (n-3 PUFAs), and especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are essential compounds for human health. They have been proven to act positively on a panel of diseases and have interesting anti-oxidative, anti-inflammatory or anti-cancer properties. For these reasons, they are receiving more and more attention in recent years, especially future food or feed development. EPA and DHA come mainly from marine sources like fish or seaweed. Unfortunately, due to global warming, these compounds are becoming scarce for humans because of overfishing and stock reduction. Although increasing in recent years, aquaculture appears insufficient to meet the increasing requirements of these healthy molecules for humans. One alternative resides in the cultivation of microalgae, the initial producers of EPA and DHA. They are also rich in biochemicals with interesting properties. After defining macro and microalgae, this review synthesizes the current knowledge on n-3 PUFAs regarding health benefits and the challenges surrounding their supply within the environmental context. Microalgae n-3 PUFA production is examined and its synthesis pathways are discussed. Finally, the use of EPA and DHA in food and feed is investigated. This work aims to define better the issues surrounding n-3 PUFA production and supply and the potential of microalgae as a sustainable source of compounds to enhance the food and feed of the future.
Effect of Ni on the Microstructure and Diffusion Behavior at the Interface of WC/High-Speed Steel Composites
Authors: Li Hongnan , Yan Ling , Meng Linghao , Li Yan , Ai Fangfang , Zhang Hongmei , Jiang Zhengyi
Published: February 2021

In this study, WC-Ni/high-speed steel composite materials for application as micro-drills were prepared by cold pressing and high-temperature vacuum sintering using a self-designed mold in the laboratory. The effect of Ni on the microstructure and diffusion behavior at the interface of the WC/high-speed steel composite was investigated. The results show that the addition of Ni promoted the diffusion of elements, and reduced defects such as micropores and microcracks at the WC/high-speed steel composite interface. It also improved the bonding strength of the WC/high-speed steel composite interface, and significantly decreased the WC hardness.
Relevance of Biofilm Models in Periodontal Research: From Static to Dynamic Systems
Authors: Sánchez María Carmen , Alonso-Español Andrea , Ribeiro-Vidal Honorato , Alonso Bettina , Herrera David , Sanz Mariano
Published: February 2021

Microbial biofilm modeling has improved in sophistication and scope, although only a limited number of standardized protocols are available. This review presents an example of a biofilm model, along with its evolution and application in studying periodontal and peri-implant diseases. In 2011, the ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) research group at the University Complutense of Madrid developed an in vitro biofilm static model using representative bacteria from the subgingival microbiota, demonstrating a pattern of bacterial colonization and maturation similar to in vivo subgingival biofilms. When the model and its methodology were standardized, the ETEP research group employed the validated in vitro biofilm model for testing in different applications. The evolution of this model is described in this manuscript, from the mere observation of biofilm growth and maturation on static models on hydroxyapatite or titanium discs, to the evaluation of the impact of dental implant surface composition and micro-structure using the dynamic biofilm model. This evolution was based on reproducing the ideal microenvironmental conditions for bacterial growth within a bioreactor and reaching the target surfaces using the fluid dynamics mimicking the salivary flow. The development of this relevant biofilm model has become a powerful tool to study the essential processes that regulate the formation and maturation of these important microbial communities, as well as their behavior when exposed to different antimicrobial compounds.