bluuebunny/crossref_metadata_2025 huggingface dataset:
Thermal Stress and Resilience of Corals in a Climate-Changing World
Authors: Carballo-Bolaños Rodrigo , Soto Derek , Chen Chaolun Allen
Published: December 2019

Coral reef ecosystems are under the direct threat of increasing atmospheric greenhouse gases, which increase seawater temperatures in the oceans and lead to bleaching events. Global bleaching events are becoming more frequent and stronger, and understanding how corals can tolerate and survive high-temperature stress should be accorded paramount priority. Here, we review evidence of the different mechanisms that corals employ to mitigate thermal stress, which include association with thermally tolerant endosymbionts, acclimatisation, and adaptation processes. These differences highlight the physiological diversity and complexity of symbiotic organisms, such as scleractinian corals, where each species (coral host and microbial endosymbionts) responds differently to thermal stress. We conclude by offering some insights into the future of coral reefs and examining the strategies scientists are leveraging to ensure the survival of this valuable ecosystem. Without a reduction in greenhouse gas emissions and a divergence from our societal dependence on fossil fuels, natural mechanisms possessed by corals might be insufficient towards ensuring the ecological functioning of coral reef ecosystems.
Organizational and Formational Structures of Networks in the Mental Lexicon: A State-Of-The-Art through Systematic Review
Authors: McCarthy Luke , Miralpeix Imma
Published: December 2019

This state-of-the-art presents a systematic exploration on the use of network patterns in global research efforts to understand, organize and represent the mental lexicon. Results have shown an increase over recent years in the usage of complex, small-world and scale-free network patterns within the literature. With the increasing complexity of network patterns, we see more potential in the inter-disciplinary exploration of the mental lexicon through universal and mathematically-describable, behavioral patterns in small-world and scale-free networks. A systematic review of 36 items of methodologically-selected literature serve as a means to explore how the greater literary body understands network structures within the mental lexicon. Network-based approaches are discriminated between three contrasting varieties. These include: ‘simple networks’, characterized by arbitrarily organized graph patterns of metaphorical importance; ‘connectionist networks’, a broad category of networks which explore the structural features of a system through the analysis of emergent properties; and lastly ‘complex networks’, distinguished as small-world, scale-free networks which follow a strict and mathematically-describable structure in agreement with the Barabási–Albert model. Each network approach is explored in terms of their discernible differences which relate to their parameters and affect their implications. A final evaluation of observed patterns within the selected literature is offered, as well as an elaboration on the sense of trajectory beheld in the research in order to offer insight and orientation for future research.
A Comprehensive Review on Bio-Nanomaterials for Medical Implants and Feasibility Studies on Fabrication of Such Implants by Additive Manufacturing Technique
Authors: Velu Rajkumar , Calais Theo , Jayakumar Arunkumar , Raspall Felix
Published: December 2019

Nanomaterials have allowed significant breakthroughs in bio-engineering and medical fields. In the present paper a holistic assessment on diverse biocompatible nanocomposites are studied. Their compatibility with advanced fabrication methods such as additive manufacturing for the design of functional medical implants is also critically reviewed. The significance of nanocomposites and processing techniques is also envisaged comprehensively in regard with the needs and futures of implantable medical device industries.
Control of Eu Oxidation State in Y2O3−xSx:Eu Thin-Film Phosphors Prepared by Atomic Layer Deposition: A Structural and Photoluminescence Study
Authors: Rosa José , Deuermeier Jonas , Soininen Pekka J. , Bosund Markus , Zhu Zhen , Fortunato Elvira , Martins Rodrigo , Sugiyama Mutsumi , Merdes Saoussen
Published: December 2019

Structural and photoluminescence studies were carried out on Eu-doped Y2O3−xSx thin films grown by atomic layer deposition at 300 °C. (CH3Cp)3Y, H2O, and H2S were used as yttrium, oxygen, and sulfur precursors, respectively, while Eu(thd)3 was used as the europium precursor. The Eu oxidation state was controlled during the growth process by following the Eu(thd)3 pulse with either a H2S or O3 pulse. The Eu(thd)3/O3 pulse sequence led to photoluminescence emission above 550 nm, whereas the Eu(thd)3/H2S pulse sequence resulted in emission below 500 nm.
Random Voids Generation and Effect of Thermal Shock Load on Mechanical Reliability of Light-Emitting Diode Flip Chip Solder Joints
Authors: Fan Jiajie , Wu Jie , Jiang Changzhen , Zhang Hao , Ibrahim Mesfin , Deng Liang
Published: December 2019

To make the light-emitting diode (LED) more compact and effective, the flip chip solder joint is recommended in LED chip-scale packaging (CSP) with critical functions in mechanical support, heat dissipation, and electrical conductivity. However, the generation of voids always challenges the mechanical strength, thermal stability, and reliability of solder joints. This paper models the 3D random voids generation in the LED flip chip Sn96.5–Ag3.0–Cu0.5 (SAC305) solder joint, and investigates the effect of thermal shock load on its mechanical reliability with both simulations and experiments referring to the JEDEC thermal shock test standard (JESD22-A106B). The results reveal the following: (1) the void rate of the solder joint increases after thermal shock ageing, and its shear strength exponentially degrades; (2) the first principal stress of the solder joint is not obviously increased, however, if the through-hole voids emerged in the corner of solder joints, it will dramatically increase; (3) modelling of the fatigue failure of solder joint with randomly distributed voids utilizes the approximate model to estimate the lifetime, and the experimental results confirm that the absolute prediction error can be controlled around 2.84%.
SiC-IrSi3 for High Oxidation Resistance
Authors: Camarano Antonio , Giuranno Donatella , Narciso Javier
Published: December 2019

SiC is a material with excellent mechanical and thermal properties but with a high production cost. Obtaining SiC by reactive infiltration is an attractive method with a much lower cost than the traditional sintering process. However, the reactive infiltration process presents a serious problem, which is the high residual silicon content, which decreases its applicability. The replacement of silicon with silicides is a widely used alternative. The present investigation shows the good mechanical properties of the SiC-IrSi3 composite material obtained by reactive infiltration of SiC-C preforms with Ir–Si alloys. The thermomechanical analysis shows a high compatibility of silicide with SiC. The presence of the silicide shows a substantial improvement against the oxidation of the SiC-Si composites.
Preference Based Portfolio for Private Investors: Discrete Choice Analysis Approach
Authors: Kuzmanovic Marija , Makajic-Nikolic Dragana , Nikolic Nebojsa
Published: December 2019

Behavioral finance literature shows that in addition to Markowitz’s rate of return and risk, private investors consider various other stock features. This paper discusses the problem of determining investors’ preferences for portfolio selection criteria, as well as the problem of optimal portfolio determination from the investors’ point of view. The study primarily focuses on private investors who are interested in one-time investments rather than stock trading. We use a discrete choice analysis and hierarchical Bayes method to measure individual investors’ preferences, and a logit model to determine individual shares of preferences. We treat the share of preferences as the share of certain stocks in an optimal portfolio. The proposed methodology is illustrated by the example of companies whose stocks are traded on the Belgrade Stock Exchange. We measure respondents’ preferences for companies, preferences for return rates, riskiness of stocks, and dividend rates. The results of comparing the performance of the resulting portfolio with the efficient frontier obtained using Markowitz’s portfolio theory indicate its high efficiency, thus validating the proposed approach.
Efficient Methods to Calculate Partial Sphere Surface Areas for a Higher Resolution Finite Volume Method for Diffusion-Reaction Systems in Biological Modeling
Authors: Bowers Abigail , Bunn Jared , Kim Myles
Published: December 2019

Computational models for multicellular biological systems, in both in vitro and in vivo environments, require solving systems of differential equations to incorporate molecular transport and their reactions such as release, uptake, or decay. Examples can be found from drugs, growth nutrients, and signaling factors. The systems of differential equations frequently fall into the category of the diffusion-reaction system due to the nature of the spatial and temporal change. Due to the complexity of equations and complexity of the modeled systems, an analytical solution for the systems of the differential equations is not possible. Therefore, numerical calculation schemes are required and have been used for multicellular biological systems such as bacterial population dynamics or cancer cell dynamics. Finite volume methods in conjunction with agent-based models have been popular choices to simulate such reaction-diffusion systems. In such implementations, the reaction occurs within each finite volume and finite volumes interact with one another following the law of diffusion. The characteristic of the reaction can be determined by the agents in the finite volume. In the case of cancer cell growth dynamics, it is observed that cell behavior can be different by a matter of a few cell size distances because of the chemical gradient. Therefore, in the modeling of such systems, the spatial resolution must be comparable to the cell size. Such spatial resolution poses an extra challenge in the development and execution of the computational model due to the agents sitting over multiple finite volumes. In this article, a few computational methods for cell surface-based reaction for the finite volume method will be introduced and tested for their performance in terms of accuracy and computation speed.
Diverse Applications of Marine Macroalgae
Authors: Leandro Adriana , Pereira Leonel , Gonçalves Ana M. M.
Published: December 2019

The aim of this paper is to review the multiplicity of the current uses of marine macroalgae. Seaweeds are already used in many products and for different purposes, from food products to medicine. They are a natural resource that can provide a number of compounds with beneficial bioactivities like antioxidant, anti-inflammatory, anti-aging effects, among others. Despite studies directed in prospecting for their properties and the commodities already marketed, they could, surely, be even more researched and sustainably explored.
New Polyketides from the Marine-Derived Fungus Letendraea Sp. 5XNZ4-2
Authors: Xu Yan , Huang Ruibao , Liu Hongwei , Yan Tingting , Ding Wanjing , Jiang Yongjun , Wang Pinmei , Zheng Daoqiong , Xu Jinzhong
Published: December 2019

Marine-derived fungi have been reported to have great potential to produce structurally unique metabolites. Our investigation on secondary metabolites from marine-derived fungi resulted in the isolation of seven new polyketides (phomopsiketones D–G (1–4) and letendronols A–C (5–7)) as well as one known xylarinol (8) in the cultural broth of Letendraea sp. Their structures and absolute configurations were elucidated using a set of spectroscopic and chemical methods, including HRESIMS, NMR, single-crystal X-ray diffraction, ECD calculation, and a modified version of Mosher’s method. Compound 2 showed weak inhibition against nitric oxide production in lipopolysaccaride-activated macrophages with an IC50 value of 86 μM.
Patients with Parkinson’s Disease and Myasthenia Gravis—A Report of Three New Cases and Review of the Literature
Authors: Odajiu Irina , Davidescu Eugenia Irene , Mitu Cristina , Popescu Bogdan Ovidiu
Published: December 2019

Neurodegenerative diseases such as Parkinson’s disease (PD)have increasing incidence, due to lifespan expansion. The association between PD and Myasthenia Gravis (MG) is uncommon, and so far, since 1987, 26 cases have been reported. We report here a series of three new cases, two men and one woman with this peculiar combination of conditions, identified in the Neurology Department of Colentina Clinical Hospital. In this article, the pathogenesis of MG in patients with PD is discussed, along with a literature review regarding the co-occurrence of these two neurological diseases.
Acupuncture for the Relief of Chronic Pain: A Synthesis of Systematic Reviews
Authors: Paley Carole A. , Johnson Mark I.
Published: December 2019

Background and Objectives: It is estimated that 28 million people in the UK live with chronic pain. A biopsychosocial approach to chronic pain is recommended which combines pharmacological interventions with behavioural and non-pharmacological treatments. Acupuncture represents one of a number of non-pharmacological interventions for pain. In the current climate of difficult commissioning decisions and constantly changing national guidance, the quest for strong supporting evidence has never been more important. Although hundreds of systematic reviews (SRs) and meta-analyses have been conducted, most have been inconclusive, and this has created uncertainty in clinical policy and practice. There is a need to bring all the evidence together for different pain conditions. The aim of this review is to synthesise SRs of RCTs evaluating the clinical efficacy of acupuncture to alleviate chronic pain and to consider the quality and adequacy of the evidence, including RCT design. Materials and Methods: Electronic databases were searched for English language SRs and meta-analyses on acupuncture for chronic pain. The SRs were scrutinised for methodology, risk of bias and judgement of efficacy. Results: A total of 177 reviews of acupuncture from 1989 to 2019 met our eligibility criteria. The majority of SRs found that RCTs of acupuncture had methodological shortcomings, including inadequate statistical power with a high risk of bias. Heterogeneity between RCTs was such that meta-analysis was often inappropriate. Conclusions: The large quantity of RCTs on acupuncture for chronic pain contained within systematic reviews provide evidence that is conflicting and inconclusive, due in part to recurring methodological shortcomings of RCTs. We suggest that an enriched enrolment with randomised withdrawal design may overcome some of these methodological shortcomings. It is essential that the quality of evidence is improved so that healthcare providers and commissioners can make informed choices on the interventions which can legitimately be provided to patients living with chronic pain.
Factors Affecting Psychological Stress in Healthcare Workers with and without Chronic Pain: A Cross-Sectional Study Using Multiple Regression Analysis
Authors: Sakamoto Yuta , Oka Takeru , Amari Takashi , Shimo Satoshi
Published: December 2019

The authors did not realize the error made in the front matter in the proofreading phase [...]
Modelling of Fracture Toughness of X80 Pipeline Steels in DTB Transition Region Involving the Effect of Temperature and Crack Growth
Authors: Xu Jie , Song Wei , Cheng Wenfeng , Chu Lingyu , Gao Hanlin , Li Pengpeng , Berto Filippo
Published: December 2019

This work presents an investigation of the effects of temperature and crack growth on cleavage fracture toughness for weld thermal simulated X80 pipeline steels in the ductile-to-brittle transition (DBT) regime. A great bulk of fracture toughness (crack tip opening displacement—CTOD) tests and numerical simulations are carried out by deep-cracked single-edge-notched bending (SENB) and shallow-cracked single-edge-notched tension (SENT) specimens at various temperatures (−90 °C, −60 °C, −30 °C, and 0 °C). Three-dimensional (3D) finite element (FE) models of tested specimens have been employed to obtain computational data. The results show that temperature exerts only a slight effect on the material hardening behavior, which indicates the crack tip constraint (as denoted by Q-parameter) is less dependent on the temperature. The measured CTOD-values give considerable scatter but confirm well-established trends of increasing toughness with increasing temperature and reducing constraint. Crack growth and 3D effect exhibited significant influences on CTOD-CMOD relations at higher temperatures, −30 °C and 0 °C for the SENT specimen.
Texture Hardening Observed in Mg–Zn–Nd Alloy Processed by Equal-Channel Angular Pressing (ECAP)
Authors: Stráská Jitka , Minárik Peter , Šašek Stanislav , Veselý Jozef , Bohlen Jan , Král Robert , Kubásek Jiří
Published: December 2019

The addition of Nd significantly improves the mechanical properties of magnesium alloys. However, only limited amounts of Nd or other rare earth (RE) elements should be used due to their high price. In this study, a low-alloyed Mg–1% Zn–1% Nd (ZN11) alloy was designed and processed by hot extrusion and subsequent equal-channel angular pressing (ECAP) in order to achieve a very fine-grained condition with enhanced strength. The microstructure, texture, and mechanical properties were thoroughly studied. The microstructure after 8 passes through ECAP was homogeneous and characterized by an average grain size of 1.5 µm. A large number of tiny secondary phase precipitates were identified as ordered Guinier–Preston (GP) zones. Detailed analysis of the Schmid factors revealed the effect of the texture on deformation mechanisms. ECAP processing more than doubled the achieved yield compression strength (YCS) of the ZN11 alloy. Significant strengthening by ECAP is caused by grain refinement and the formation of ordered Guinier–Preston zones and particles of a secondary γ-phase.
Generation of a Collision Cross Section Library for Multi-Dimensional Plant Metabolomics Using UHPLC-Trapped Ion Mobility-MS/MS
Authors: Schroeder Mark , Meyer Sven W. , Heyman Heino M. , Barsch Aiko , Sumner Lloyd W.
Published: December 2019

The utility of metabolomics is well documented; however, its full scientific promise has not yet been realized due to multiple technical challenges. These grand challenges include accurate chemical identification of all observable metabolites and the limiting depth-of-coverage of current metabolomics methods. Here, we report a combinatorial solution to aid in both grand challenges using UHPLC-trapped ion mobility spectrometry coupled to tandem mass spectrometry (UHPLC-TIMS-TOF-MS). TIMS offers additional depth-of-coverage through increased peak capacities realized with the multi-dimensional UHPLC-TIMS separations. Metabolite identification confidence is simultaneously enhanced by incorporating orthogonal collision cross section (CCS) data matching. To facilitate metabolite identifications, we created a CCS library of 146 plant natural products. This library was generated using TIMS with N2 drift gas to record the TIMSCCSN2 of plant natural products with a high degree of reproducibility; i.e., average RSD = 0.10%. The robustness of TIMSCCSN2 data matching was tested using authentic standards spiked into complex plant extracts, and the precision of CCS measurements were determined to be independent of matrix affects. The utility of the UHPLC-TIMS-TOF-MS/MS in metabolomics was then demonstrated using extracts from the model legume Medicago truncatula and metabolites were confidently identified based on retention time, accurate mass, molecular formula, and CCS.
Design and Fabrication of Dual-Scale Broadband Antireflective Structures on Metal Surfaces by Using Nanosecond and Femtosecond Lasers
Authors: Lou Rui , Zhang Guodong , Li Guangying , Li Xuelong , Liu Qing , Cheng Guanghua
Published: December 2019

Antireflective surfaces, with their great potential applications, have attracted tremendous attention and have been the subject of extensive research in recent years. However, due to the significant optical impedance mismatch between a metal surface and free space, it is still a challenging issue to realize ultralow reflectance on a metal surface. To address this issue, we propose a two-step strategy for constructing antireflective structures on a Ti-6Al-4V (TC4) surface using nanosecond and femtosecond pulsed lasers in combination. By controlling the parameters of the nanosecond laser, microgrooves are first scratched on the TC4 surface to reduce the interface reflection. Then, the femtosecond laser is focused onto the sample surface with orthogonal scanning to induce deep air holes and nanoscale structures, which effectively enhances the broadband absorption. The antireflection mechanism of the dual-scale structures is discussed regarding morphological characterization and hemispherical reflectance measurements. Finally, the modified sample surface covered with micro-nano hybrid structures is characterized by an average reflectance of 3.1% over the wavelengths ranging from 250 nm to 2250 nm.
Modeling and Analysis of the Two-Dimensional Axisymmetric Acoustofluidic Fields in the Probe-Type and Substrate-Type Ultrasonic Micro/Nano Manipulation Systems
Authors: Liu Pengzhan , Tang Qiang , Su Songfei , Hu Jie , Yu Yang
Published: December 2019

The probe-type and substrate-type ultrasonic micro/nano manipulation systems have proven to be two kinds of powerful tools for manipulating micro/nanoscale materials. Numerical simulations of acoustofluidic fields in these two kinds of systems can not only be used to explain and analyze the physical mechanisms of experimental phenomena, but also provide guidelines for optimization of device parameters and working conditions. However, in-depth quantitative study and analysis of acoustofluidic fields in the two ultrasonic micro/nano manipulation systems have scarcely been reported. In this paper, based on the finite element method (FEM), we numerically investigated the two-dimensional (2D) axisymmetric acoustofluidic fields in the probe-type and substrate-type ultrasonic micro/nano manipulation systems by the perturbation method (PM) and Reynolds stress method (RSM), respectively. Through comparing the simulation results computed by the two methods and the experimental verifications, the feasibility and reasonability of the two methods in simulating the acoustofluidic fields in these two ultrasonic micro/nano manipulation systems have been validated. Moreover, the effects of device parameters and working conditions on the acoustofluidic fields are clarified by the simulation results and qualitatively verified by the experiments.
Nanoantenna Structure with Mid-Infrared Plasmonic Niobium-Doped Titanium Oxide
Authors: Ngo Hai Dang , Chen Kai , Handegård Ørjan S. , Doan Anh Tung , Ngo Thien Duc , Dao Thang Duy , Ikeda Naoki , Ohi Akihiko , Nabatame Toshihide , Nagao Tadaaki
Published: December 2019

Among conductive oxide materials, niobium doped titanium dioxide has recently emerged as a stimulating and promising contestant for numerous applications. With carrier concentration tunability, high thermal stability, mechanical and environmental robustness, this is a material-of-choice for infrared plasmonics, which can substitute indium tin oxide (ITO). In this report, to illustrate great advantages of this material, we describe successful fabrication and characterization of niobium doped titanium oxide nanoantenna arrays aiming at surface-enhanced infrared absorption spectroscopy. The niobium doped titanium oxide film was deposited with co-sputtering method. Then the nanopatterned arrays were prepared by electron beam lithography combined with plasma etching and oxygen plasma ashing processes. The relative transmittance of the nanostrip and nanodisk antenna arrays was evaluated with Fourier transform infrared spectroscopy. Polarization dependence of surface plasmon resonances on incident light was examined confirming good agreements with calculations. Simulated spectra also present red-shift as length, width or diameter of the nanostructures increase, as predicted by classical antenna theory.
Arced Multi-Nozzle Electrospinning Spinneret for High-Throughput Production of Nanofibers
Authors: Jiang Jiaxin , Zheng Gaofeng , Wang Xiang , Li Wenwang , Kang Guoyi , Chen Huatan , Guo Shumin , Liu Juan
Published: December 2019

The stable and continuous ejection of multiple jets with high densities is the key to the application of electrospinning technology. An arced multi-nozzle spinneret was designed to increase the production efficiency of electrospinning. The distribution of the electrical field was simulated to optimize the nozzles’ distribution of the spinneret. When the nozzles were arranged in an arc array, a relatively uniform electrical field could be obtained, which was beneficial for the weakening of electrical interference among the nozzles. Under the optimized electrical field, multiple jets from each nozzle could be ejected in a stable and continuous way. With the increase of the applied voltage, the electrical stretching force became larger, and there were fewer bonding structures. The average diameter of the electrospun nanofibers decreased with the increase of the applied voltage. When the distance between the inner nozzle and the collector increased, the charged jets suffered a larger stretching effect, resulting in the decrease of the average diameter of the electrospun nanofibers. The electrospinning current increased with the applied voltage and decreased with the distance between the inner nozzle and the collector, which is an important aspect for the monitoring of electrospinning jets. This work provides an effective way to promote the production efficiency of electrospun nanofibrous membranes.
VelA and LaeA are Key Regulators of Epichloë festucae Transcriptomic Response during Symbiosis with Perennial Ryegrass
Authors: Rahnama Mostafa , Maclean Paul , Fleetwood Damien J. , Johnson Richard D.
Published: December 2019

VelA (or VeA) is a key global regulator in fungal secondary metabolism and development which we previously showed is required during the symbiotic interaction of Epichloë festucae with perennial ryegrass. In this study, comparative transcriptomic analyses of ∆velA mutant compared to wild-type E. festucae, under three different conditions (in culture, infected seedlings, and infected mature plants), were performed to investigate the impact of VelA on E. festucae transcriptome. These comparative transcriptomic studies showed that VelA regulates the expression of genes encoding proteins involved in membrane transport, fungal cell wall biosynthesis, host cell wall degradation, and secondary metabolism, along with a number of small secreted proteins and a large number of proteins with no predictable functions. In addition, these results were compared with previous transcriptomic experiments that studied the impact of LaeA, another key global regulator of secondary metabolism and development that we have shown is important for E. festucae–perennial ryegrass interaction. The results showed that although VelA and LaeA regulate a subset of E. festucae genes in a similar manner, they also regulated many other genes independently of each other suggesting specialised roles.
Hafnia alvei HA4597 Strain Reduces Food Intake and Body Weight Gain and Improves Body Composition, Glucose, and Lipid Metabolism in a Mouse Model of Hyperphagic Obesity
Authors: Lucas Nicolas , Legrand Romain , Deroissart Camille , Dominique Manon , Azhar Saïda , Le Solliec Marie-Anne , Léon Fatima , do Rego Jean-Claude , Déchelotte Pierre , Fetissov Sergueï O. , Lambert Grégory
Published: December 2019

Use of new generation probiotics may become an integral part of the prevention and treatment strategies of obesity. The aim of the present study was to test the efficacy of a potential probiotic strain of lactic bacteria Hafnia alvei (H. alvei) HA4597™, in a mouse model of obesity characterized by both hyperphagia and diet-induced adiposity. For this purpose, 10-week-old high-fat-diet (HFD)-fed hyperphagic ob/ob male mice received a daily treatment with 1.4 × 1010 CFU of H. alvei for 38 days. Effects of H. alvei were compared to those of a lipase inhibitor orlistat (80 mg/kg daily) and a vehicle (NaCl 0.9%) in HFD-fed ob/ob mice. A control untreated group of ob/ob mice received the standard diet throughout the experiment. The vehicle-treated HFD group displayed increased food intake, worsening of adiposity, and glycemia. Treatment with H. alvei was accompanied by decreased body weight and fat-mass gain along with reduced food intake to the level of the standard-diet-fed mice. At the end of the experiment, the group treated with H. alvei showed a decrease of glycemia, plasma total cholesterol, and alanine aminotransferase. The orlistat-treated mice showed a lower rate of body weight gain but were hyperphagic and hyperglycemic. These results demonstrate the beneficial anti-obesity and metabolic effects of H. alvei HA4597™ in mice with obesity resulting from hyperphagia and diet-induced adiposity.
Prevalence of Non-B HIV-1 Subtypes in North Italy and Analysis of Transmission Clusters Based on Sequence Data Analysis
Authors: Lorenzin Giovanni , Gargiulo Franco , Caruso Arnaldo , Caccuri Francesca , Focà Emanuele , Celotti Anna , Quiros-Roldan Eugenia , Izzo Ilaria , Castelli Francesco , De Francesco Maria A.
Published: December 2019

HIV-1 diversity is increasing in European countries due to immigration flows, as well as travels and human mobility, leading to the circulation of both new viral subtypes and new recombinant forms, with important implications for public health. We analyzed 710 HIV-1 sequences comprising protease and reverse-transcriptase (PR/RT) coding regions, sampled from 2011 to 2017, from naive patients in Spedali Civili Hospital, Brescia, Italy. Subtyping was performed by using a combination of different tools; the phylogenetic analysis with a structured coalescence model and Makarov Chain Monte Carlo was used on the datasets, to determine clusters and evolution. We detected 304 (43%) patients infected with HIV-1 non-B variants, of which only 293 sequences were available, with four pure subtypes and five recombinant forms; subtype F1 (17%) and CRF02_AG (51.1%) were most common. Twenty-five transmission clusters were identified, three of which included >10 patients, belonging to subtype CRF02_AG and subtype F. Most cases of alleged transmission were between heterosexual couples. Probably due to strong migratory flows, we have identified different subtypes with particular patterns of recombination or, as in the case of the subtype G (18/293, 6.1%), to a complete lack of relationship between the sequenced strains, revealing that they are all singletons. Continued HIV molecular surveillance is most important to analyze the dynamics of the boost of transmission clusters in order to implement public health interventions aimed at controlling the HIV epidemic.
Evolutionary Engineering of an Iron-Resistant Saccharomyces cerevisiae Mutant and Its Physiological and Molecular Characterization
Authors: Balaban Berrak Gülçin , Yılmaz Ülkü , Alkım Ceren , Topaloğlu Alican , Kısakesen Halil İbrahim , Holyavkin Can , Çakar Zeynep Petek
Published: December 2019

Iron plays an essential role in all organisms and is involved in the structure of many biomolecules. It also regulates the Fenton reaction where highly reactive hydroxyl radicals occur. Iron is also important for microbial biodiversity, health and nutrition. Excessive iron levels can cause oxidative damage in cells. Saccharomyces cerevisiae evolved mechanisms to regulate its iron levels. To study the iron stress resistance in S. cerevisiae, evolutionary engineering was employed. The evolved iron stress-resistant mutant “M8FE” was analysed physiologically, transcriptomically and by whole genome re-sequencing. M8FE showed cross-resistance to other transition metals: cobalt, chromium and nickel and seemed to cope with the iron stress by both avoidance and sequestration strategies. PHO84, encoding the high-affinity phosphate transporter, was the most down-regulated gene in the mutant, and may be crucial in iron-resistance. M8FE had upregulated many oxidative stress response, reserve carbohydrate metabolism and mitophagy genes, while ribosome biogenesis genes were downregulated. As a possible result of the induced oxidative stress response genes, lower intracellular oxidation levels were observed. M8FE also had high trehalose and glycerol production levels. Genome re-sequencing analyses revealed several mutations associated with diverse cellular and metabolic processes, like cell division, phosphate-mediated signalling, cell wall integrity and multidrug transporters.
Isolation and Characterization of Genes Responsible for Naphthalene Degradation from Thermophilic Naphthalene Degrader, Geobacillus sp. JF8
Authors: Miyazawa Daisuke , Thanh Le Thi Ha , Tani Akio , Shintani Masaki , Loc Nguyen Hoang , Hatta Takashi , Kimbara Kazuhide
Published: December 2019

Geobacillus sp. JF8 is a thermophilic biphenyl and naphthalene degrader. To identify the naphthalene degradation genes, cis-naphthalene dihydrodiol dehydrogenase was purified from naphthalene-grown cells, and its N-terminal amino acid sequence was determined. Using a DNA probe encoding the N-terminal region of the dehydrogenase, a 10-kb DNA fragment was isolated. Upstream of nahB, a gene for dehydrogenase, there were two open reading frames which were designated as nahAc and nahAd, respectively. The products of nahAc and nahAd were predicted to be alpha and beta subunit of ring-hydroxylating dioxygenases, respectively. Phylogenetic analysis of amino acid sequences of NahB indicated that it did not belong to the cis-dihydrodiol dehydrogenase group that includes those of classical naphthalene degradation pathways. Downstream of nahB, four open reading frames were found, and their products were predicted as meta-cleavage product hydrolase, monooxygenase, dehydrogenase, and gentisate 1,2-dioxygenase, respectively. A reverse transcriptase-PCR analysis showed that transcription of nahAcAd was induced by naphthalene. These findings indicate that we successfully identified genes involved in the upper pathway of naphthalene degradation from a thermophilic bacterium.
By-Products of Camu-Camu [Myrciaria dubia (Kunth) McVaugh] as Promising Sources of Bioactive High Added-Value Food Ingredients: Functionalization of Yogurts
Authors: Conceição Natália , Albuquerque Bianca R. , Pereira Carla , Corrêa Rúbia C. G. , Lopes Camila B. , Calhelha Ricardo C. , Alves Maria José , Barros Lillian , C. F. R. Ferreira Isabel
Published: December 2019

Camu-camu (Myrciaria dubia (Kunth) McVaugh) is a fruit economically relevant to the Amazon region, mostly consumed in the form of processed pulp. Our aim was to perform an unprecedented comparative study on the chemical composition and bioactivities of the camu-camu pulp and industrial bio-residues (peel and seed), and then the most promising fruit part was further explored as a functionalized ingredient in yogurt. A total of twenty-three phenolic compounds were identified, with myricetin-O-pentoside and cyanindin-3-O-glucoside being the main compounds in peels, followed by p-coumaroyl hexoside in the pulp, and ellagic acid in the seeds. The peel displayed the richest phenolic profile among samples, as well as the most significant antibacterial (MICs = 0.625–10 mg/mL) and anti-proliferative (GI50 = 180 µg/mL against HeLa cells) activities. For this reason, it was selected to be introduced in a food system (yogurt). Taken together, our results suggest the possibility of using the camu-camu peel as a source of food additives.
Molecular Mechanism Underlying Hypoxic Preconditioning-Promoted Mitochondrial Translocation of DJ-1 in Hypoxia/Reoxygenation H9c2 Cells
Authors: Deng Yi-Zhang , Xiao Lin , Zhao Le , Qiu Le-Jia , Ma Zhao-Xia , Xu Xing-Wang , Liu Hao-Yue , Zhou Ting-Ting , Wang Xue-Ying , Tang Lei , Chen He-Ping
Published: December 2019

DJ-1 was recently reported to be involved in the cardioprotection of hypoxic preconditioning (HPC) against hypoxia/reoxygenation (H/R)-induced oxidative stress damage, by preserving mitochondrial complex I activity and, subsequently, inhibiting mitochondrial reactive oxygen species (ROS) generation. However, the molecular mechanism by which HPC enables mitochondrial translocation of DJ-1, which has no mitochondria-targeting sequence, to preserve mitochondrial complex I, is largely unknown. In this study, co-immunoprecipitation data showed that DJ-1 was associated with glucose-regulated protein 75 (Grp75), and this association was significantly enhanced after HPC. Immunofluorescence imaging and Western blot analysis showed that HPC substantially enhanced the translocation of DJ-1 from cytosol to mitochondria in H9c2 cells subjected to H/R, which was mimicked by DJ-1 overexpression induced by pFlag-DJ-1 transfection. Importantly, knockdown of Grp75 markedly reduced the mitochondrial translocation of DJ-1 induced by HPC and pFlag-DJ-1 transfection. Moreover, HPC promoted the association of DJ-1 with mitochondrial complex I subunits ND1 and NDUFA4, improved complex I activity, and inhibited mitochondria-derived ROS production and subsequent oxidative stress damage after H/R, which was also mimicked by pFlag-DJ-1 transfection. Intriguingly, these effects of HPC and pFlag-DJ-1 transfection were also prevented by Grp75 knockdown. In conclusion, these results indicated that HPC promotes the translocation of DJ-1 from cytosol to mitochondria in a Grp75-dependent manner and Grp75 is required for DJ-1-mediated protection of HPC on H/R-induced mitochondrial complex I defect and subsequent oxidative stress damage.
Synthesis and Dissolution of Metal Oxides in Ionic Liquids and Deep Eutectic Solvents
Authors: Richter Janine , Ruck Michael
Published: December 2019

Ionic liquids (ILs) and deep eutectic solvents (DESs) have proven to be suitable solvents and reactants for low-temperature reactions. To date, several attempts were made to apply this promising class of materials to metal oxide chemistry, which, conventionally, is performed at high temperatures. This review gives an overview about the scientific approaches of the synthesis as well as the dissolution of metal oxides in ILs and DESs. A wide range of metal oxides along with numerous ILs and DESs are covered by this research. With ILs and DESs being involved, many metal oxide phases as well as different particle morphologies were obtained by means of relatively simple reactions paths. By the development of acidic task-specific ILs and DESs, even difficultly soluble metal oxides were dissolved and, hence, made accessible for downstream chemistry. Especially the role of ILs in these reactions is in the focus of discussion.
Preparation of Ionic Liquid-Coated Graphene Nanosheets/PTFE Nanocomposite for Stretchable, Flexible Conductor via a Pre-Stretch Processing
Authors: Zhang Yu , Kou Kaichang , Ji Tiezheng , Huang Zhengyong , Zhang Shuangcun , Zhang Shijie , Wu Guanglei
Published: December 2019

The various volume concentrations of ionic liquid-modified graphene nanosheets filled polytetrafluoroethylene nanocomposites (IL-GNs/PTFE) for flexible conductors were fabricated via a pre-stretch processing method after cold-press sintering. The results indicated that pre-stretching has no significant weakening in the electrical conductivity of the nanocomposites, while the Young’s modulus greatly reduced by 62.5%, which is more suitable for flexible conductors. This may be because the reduced conductivity by the destructive conductive pathway cancels out the enhanced conductivity by the increased interlamellar spacing of IL-GNs via a pre-stretch processing, and the nanocomposite exhibits a phase transition from two to three-phase (with the introduction of an air phase) during pre-stretching. It was also found that the tensile strength of the nanocomposites was enhanced by 42.9% and the elongation at break and thermal conductivity decreased slightly with the same filler content after pre-stretching. The electrical conductivity of the pre-stretched nanocomposites tended to stabilize at 5.5 × 10−2 s·m−1, when the volume content of the packings achieved a percolation threshold (1.49 vol%). Meanwhile, the electrical resistivity of the pre-stretched 3.0 vol% IL-GNs/PTFE nanocomposite was slightly reduced by 0.30%, 0.38%, and 0.87% respectively after 180° twisting, 180° bending, and 10% stretching strain for 1000 cycles.
Self-Alignment of Bottom CZTSSe by Patterning of an Al2O3 Intermediate Layer
Authors: Hong Sanghun , Kim Se-Yun , Son Dae-Ho , Kim Seung-Hyun , Kim Young-Ill , Yang Kee-Jeong , Heo Young-Woo , Kang Jin-Kyu , Kim Dae-Hwan
Published: December 2019

When CZTSSe is synthesized using a metal precursor, large voids of nonuniform size form at Mo back contact side. Herein, we demonstrate that the voids and CZTSSe in the lower part of the CZTSSe double layer can be controlled by using an Al2O3-patterned Mo substrate. The CZTSSe in the lower part self-aligns on the Mo-exposed area, while the voids self-align on the Al2O3-coated area. The origin of the self-alignment is expected to be the difference in bonding characteristics between liquid Sn and the metal or oxide surface, e.g., Al2O3. Good wettability generally forms between nonreactive liquid metals and metal surfaces due to the strong metallic bonding. By contrast, poor wettability generally forms between nonreactive liquid metals and oxide surfaces due to the weak van der Waals bonding between the liquid metal and the oxide layer. When the patterning was added, the device efficiency tended to decrease from 8.6% to 10.5%.
Ultrasensitive SERS-Based Plasmonic Sensor with Analyte Enrichment System Produced by Direct Laser Writing
Authors: Pavliuk Georgii , Pavlov Dmitrii , Mitsai Eugeny , Vitrik Oleg , Mironenko Aleksandr , Zakharenko Alexander , Kulinich Sergei A. , Juodkazis Saulius , Bratskaya Svetlana , Zhizhchenko Alexey , Kuchmizhak Aleksandr
Published: December 2019

We report an easy-to-implement device for surface-enhanced Raman scattering (SERS)-based detection of various analytes dissolved in water droplets at trace concentrations. The device combines an analyte-enrichment system and SERS-active sensor site, both produced via inexpensive and high-performance direct femtosecond (fs)-laser printing. Fabricated on a surface of water-repellent polytetrafluoroethylene substrate as an arrangement of micropillars, the analyte-enrichment system supports evaporating water droplet in the Cassie–Baxter superhydrophobic state, thus ensuring delivery of the dissolved analyte molecules towards the hydrophilic SERS-active site. The efficient pre-concentration of the analyte onto the sensor site based on densely arranged spiky plasmonic nanotextures results in its subsequent label-free identification by means of SERS spectroscopy. Using the proposed device, we demonstrate reliable SERS-based fingerprinting of various analytes, including common organic dyes and medical drugs at ppb concentrations. The proposed device is believed to find applications in various areas, including label-free environmental monitoring, medical diagnostics, and forensics.
Associations between Dietary Patterns and Bile Acids—Results from a Cross-Sectional Study in Vegans and Omnivores
Authors: Trefflich Iris , Marschall Hanns-Ulrich , Giuseppe Romina di , Ståhlman Marcus , Michalsen Andreas , Lampen Alfonso , Abraham Klaus , Weikert Cornelia
Published: December 2019

Bile acids play an active role in fat metabolism and, in high-fat diets, elevated concentrations of fecal bile acids may be related to an increased risk of colorectal cancer. This study investigated concentrations of fecal and serum bile acids in 36 vegans and 36 omnivores. The reduced rank regression was used to identify dietary patterns associated with fecal bile acids. Dietary patterns were derived with secondary and conjugated fecal bile acids as response variables and 53 food groups as predictors. Vegans had higher fiber (p < 0.01) and lower fat (p = 0.0024) intake than omnivores. In serum, primary and glycine-conjugated bile acids were higher in vegans than in omnivores (p ≤ 0.01). All fecal bile acids were significantly lower in vegans compared to omnivores (p < 0.01). Processed meat, fried potatoes, fish, margarine, and coffee contributed most positively, whereas muesli most negatively to a dietary pattern that was directly associated with all fecal bile acids. According to the pattern, fat intake was positively and fiber intake was inversely correlated with bile acids. The findings contribute to the evidence that, in particular, animal products and fat may play a part in higher levels of fecal bile acids.
Effects of Preventive Nutrition Interventions among Adolescents on Health and Nutritional Status in Low- and Middle-Income Countries: A Systematic Review and Meta-Analysis
Authors: Salam Rehana A , Das Jai K , Ahmed Wardah , Irfan Omar , Sheikh Sana Sadiq , Bhutta Zulfiqar A
Published: December 2019

The objective of this review was to assess the impact of preventive nutrition interventions on health and nutritional status of adolescents aged 10–19 years in low- and middle-income countries (LMICs). We searched the databases until 5 February 2019 without any restrictions on publication, date, language, or publication status. A total of 10 studies (15 papers) including 10,802 participants assessing the impact of micronutrient supplementation/fortification were included in this review. We did not find any study assessing the impact of nutrition education and counseling or macronutrient supplementation among adolescents. Among primary outcomes, we are uncertain of the effect of iron supplementation with or without folic acid on anemia (daily supplementation; relative risk (RR): 1.04, 95% confidence interval (CI) 0.42, 2.57; one study; 1160 participants; low-quality evidence; weekly supplementation; RR: 1.07, 95% CI: 0.46, 2.52; one study; 1247 participants; low-quality evidence). We are also uncertain of the effect of various micronutrient supplementation/fortification on body mass index (BMI) (calcium/vitamin D supplementation; (MD: −0.01 kg/m2; 95% CI: −1.20, 1.17; two studies; 730 participants; I2 94%; very-low-quality evidence, iron supplementation with or without folic acid; MD: 0.47 kg/m2; 95% CI: −0.17, 1.11; two studies; 652 participants; I2 37%; very-low-quality evidence, zinc supplementation; MD: 0.35 kg/m2; 95% CI: −0.15, 0.85; one study; 382 participants; very-low-quality evidence) and multiple micronutrient (MMN) fortification; MD: 0.23 kg/m2, 95% CI: −0.11, 0.57; two studies; 943 participants; I2 22%; very-low-quality evidence). None of the included studies reported any other primary outcomes including morbidity or adverse effects. Among secondary outcomes, iron supplementation with or without folic acid may improve hemoglobin concentrations, and calcium/vitamin D supplementation may improve serum 25(OH)D levels, while calcium only supplementation and calcium and vitamin D supplementation may marginally improve total body bone mineral density (BMD). We are uncertain of the effect of MMN fortification on hemoglobin concentrations, calcium supplementation on total body bone mineral content (BMC), calcium + vitamin D supplementation on total body BMC, and zinc supplementation on zinc levels. There is limited evidence of micronutrient supplementation/fortification among adolescents, especially adolescent boys, on health and nutritional status in LMICs. These findings should be interpreted with caution due to the low quality and limited number of studies.