bluuebunny/crossref_metadata_2025 huggingface dataset:
Combustion Performance of Various Polylactic Acid Plastics with Different Porous Structures Constructed by 3D Printing
Authors: Yuan Chao , Wang Yachao
Published: November 2023

Polylactic acid (PLA) has intrigued widespread attention as a biodegradable and environmentally friendly polymer, and recent research has revealed that the use of porous PLA in heat sinks for thermal management materials offers promising development potential. However, the heat transfer performance is closely related to its structure theoretically, whether it is virgin, and how the pore structure affects its heat transfer. Therefore, a novel approach is proposed to address this issue by preparing porous PLA through 3D printing at low complexity and cost, the combustion performance is employed to evaluate the heat transfer indirectly, and the higher burning speed represents higher efficient heat transfer. A new framework is developed to investigate combustion performance and three series of PLA with different pore structures in pore shape, size, and interval are studied by combining experimental tests, respectively. It demonstrates that adjusting the pore structure of PLA significantly alters its combustion performance, evidenced by significant variations in flame growth index, which are 83% better for the 2 mm holes than the largest holes and 71% better for the 2 mm interval than for the sparsest pore structure. Generally, it provides some experimental basis for designing porous thermal management materials; the various pore structures generate different combustion performances, corresponding to various heat transfer.
Updated Land Use and Land Cover Information Improves Biomass Burning Emission Estimates
Authors: Mataveli Guilherme , Pereira Gabriel , Sanchez Alber , de Oliveira Gabriel , Jones Matthew W. , Freitas Saulo R. , Aragão Luiz E. O. C.
Published: November 2023

Biomass burning (BB) emissions negatively impact the biosphere and human lives. Orbital remote sensing and modelling are used to estimate BB emissions on regional to global scales, but these estimates are subject to errors related to the parameters, data, and methods available. For example, emission factors (mass emitted by species during BB per mass of dry matter burned) are based on land use and land cover (LULC) classifications that vary considerably across products. In this work, we evaluate how BB emissions vary in the PREP-CHEM-SRC emission estimator tool (version 1.8.3) when it is run with original LULC data from MDC12Q1 (collection 5.1) and newer LULC data from MapBiomas (collection 6.0). We compare the results using both datasets in the Brazilian Amazon and Cerrado biomes during the 2002–2020 time series. A major reallocation of emissions occurs within Brazil when using the MapBiomas product, with emissions decreasing by 788 Gg (−1.91% year−1) in the Amazon and emissions increasing by 371 Gg (2.44% year−1) in the Cerrado. The differences identified are mostly associated with the better capture of the deforestation process in the Amazon and forest formations in Northern Cerrado with the MapBiomas product, as emissions in forest-related LULCs decreased by 5260 Gg in the Amazon biome and increased by 1676 Gg in the Cerrado biome. This is an important improvement to PREP-CHEM-SRC, which could be considered the tool to build South America’s official BB emission inventory and to provide a basis for setting emission reduction targets and assessing the effectiveness of mitigation strategies.
The Physical and Structural Effects of 1-MCP on Four Different Apple Cultivars during Storage
Authors: Ting Valentina J. L. , Silcock Pat , Biasioli Franco , Bremer Phil
Published: November 2023

The impact of the ethylene inhibitor, 1-methylcyclopropene (1-MCP), on four apple cultivars (Braeburn, Fuji, Jazz and Golden Delicious) over 150 days of storage at 2 °C was assessed. Proton transfer reaction quadrupole mass spectrometry (PTR-QUAD-MS) was used to monitor changes in VOC composition, while texture analysis and X-ray microcomputer tomography (µ-CT) scanning were used to study microstructural changes. The application of 1-MCP on apples reduced VOC emissions, concurrently maintaining a firmer texture compared to the untreated apples at each time point. The µ-CT scanning revealed how changes in specific morphological characteristics such as anisotropy, connectivity and porosity, size and shape, as well as the interconnectivity of intracellular spaces (IS) influenced texture even when porosity was similar. Additionally, this study showed that the porosity and connectivity of IS were associated with VOC emission and increased simultaneously. This study highlights how the morphological parameters of an apple can help explain their ripening process during long-term storage and how their microstructure can influence the release of VOCs.
Latest Advances in Active Materials for Food Packaging and Their Application
Authors: Jiang Yongli , Zhang Yifeng , Deng Yun
Published: November 2023

Food packaging plays a pivotal role in preserving the quality and safety of food products while extending their shelf life [...]
Marijuana: Forensics of Abuse, Medical Uses, Controversy, and AI
Authors: Brown Olen R.
Published: November 2023

Motor vehicle accidents are a significant consequence of marijuana abuse. Limitations of its roadside detection and the forensic problems with establishing impairment require innovations that are scientifically achievable. Marijuana abuse currently is at an all-time high in the United States and its physiological effects make it a popular recreational drug that is reported to be a leading cause of preventable morbidity and mortality among the youth in the 18 most affluent nations. The medical benefits of drugs derived from marijuana complicate its forensic regulation. In an extensive 2017 report by The American Academy of Sciences, the evidence for the medical benefits of delta-9 tetrahydrocannabinol (derived from marijuana) were stated to be conclusive or substantial for the treatment of chronic pain in adults, as an antiemetic for treatment of chemotherapy-induced nausea and vomiting, and for improving patient-reported multiple sclerosis spasticity symptoms. The benefits from treatment for many other medical conditions were inconclusive or absent. The evidence is clear that safely driving a motor vehicle is significantly impaired while under the influence of marijuana. However, there is no roadside forensic test that reliably detects impairment, and there is an urgent need for such to protect the public while insuring the legal rights of users. There is strong societal pressure to relax marijuana’s forensic regulation, including removing it from Schedule I of the Controlled Substances Act. Artificial intelligence (AI) should be implemented with appropriate human control to resolve controversies.
On the Possible Asymmetry in Gamma Rays from Andromeda Due to Inverse Compton Scattering of Star Light on Electrons from Dark Matter Annihilation
Authors: Belotsky Konstantin , Solovyov Maxim
Published: November 2023

Dark matter is a popular candidate to a new source of primary-charged particles, especially positrons in cosmic rays, which are proposed to account for observable anomalies. While this hypothesis of decaying or annihilating DM is mostly applied for our Galaxy, it could possibly lead to some interesting phenomena when applied for the other ones. In this work, we look into the hypothetical asymmetry in gamma radiation from the upper and lower hemisphere of the dark matter halo of the Andromeda galaxy due to inverse Compton scattering of starlight on the DM-produced electrons and positrons. While our 2D toy model raises expectations for the possible effect, a more complex approach gives negligible effect for the dark halo case, but shows some prospects for a dark disk model.
Uncoupling Protein 3 Promotes the Myogenic Differentiation of Type IIb Myotubes in C2C12 Cells
Authors: You Ziwei , Wang Jieyu , Li Faliang , Hei Wei , Li Meng , Guo Xiaohong , Gao Pengfei , Cao Guoqing , Cai Chunbo , Li Bugao
Published: November 2023

Uncoupling protein 3 (Ucp3) is an important transporter within mitochondria and is mainly expressed in skeletal muscle, brown adipose tissue and the myocardium. However, the effects of Ucp3 on myogenic differentiation are still unclear. This study evaluated the effects of Ucp3 on myogenic differentiation, myofiber type and energy metabolism in C2C12 cells. Gain- and loss-of-function studies revealed that Ucp3 could increase the number of myotubes and promote the myogenic differentiation of C2C12 cells. Furthermore, Ucp3 promoted the expression of the type IIb myofiber marker gene myosin heavy chain 4 (Myh4) and decreased the expression of the type I myofiber marker gene myosin heavy chain 7 (Myh7). In addition, energy metabolism related to the expression of PPARG coactivator 1 alpha (Pgc1-α), ATP synthase, H+ transportation, mitochondrial F1 complex, alpha subunit 1 (Atp5a1), lactate dehydrogenase A (Ldha) and lactate dehydrogenase B (Ldhb) increased with Ucp3 overexpression. Ucp3 could promote the myogenic differentiation of type IIb myotubes and accelerate energy metabolism in C2C12 cells. This study can provide the theoretical basis for understanding the role of Ucp3 in energy metabolism.
Penaeid Shrimp Chromosome Studies Entering the Post-Genomic Era
Authors: Zhang Xiaojun , Xiang Jianhai , Yuan Jianbo , Li Fuhua
Published: November 2023

Chromosome studies provide the foundation for comprehending inheritance, variation, systematics, and evolution. Penaeid shrimps are a group of crustaceans with great economic importance. Basic cytogenetic information obtained from these shrimps can be used to study their genome structure, chromosome relationships, chromosome variation, polyploidy manipulation, and breeding. The study of shrimp chromosomes experienced significant growth in the 1990s and has been closely linked to the progress of genome research since the application of next-generation sequencing technology. To date, the genome sequences of five penaeid shrimp species have been published. The availability of these genomes has ushered the study of shrimp chromosomes into the post-genomic era. Currently, research on shrimp cytogenetics not only involves chromosome counting and karyotyping, but also extends to investigating submicroscopic changes; exploring genome structure and regulation during various cell divisions; and contributing to the understanding of mechanisms related to growth, sexual control, stress resistance, and genome evolution. In this article, we provide an overview of the progress made in chromosome research on penaeid shrimp. We emphasize the mutual promotion between studies on chromosome structure and genome research and highlight the impact of chromosome-level assembly on studies of genome structure and function. Additionally, we summarize the emerging trends in post-genomic-era shrimp chromosome research.
The Quality of Telenursing—Israeli Nursing Staff’s Perceptions
Authors: Grinberg Keren , Sela Yael
Published: November 2023

Background: The outbreak of the COVID-19 pandemic has increased telemedicine and telenursing services worldwide, developed this innovative treatment’s potential, and emphasized its importance. The constraints imposed by the pandemic breached regulatory, psychological, and organizational obstructions among both patients and caretakers. Community and hospital nursing services were forced to deal with a new reality, to provide remote care solutions for bedridden chronic patients, as the need for this grew exponentially. Despite the increase of telemedicine in recent years, so far no research in Israel has investigated the nursing staff’s perceptions of the quality of the care provided through telenursing. Objective: To assess nurses’ perceptions of the quality of the care provided through telenursing compared to face-to-face nursing. Method: A quantitative cross-sectional study among 227 male and female nurses in Israel. The questionnaire included demographic questions, and the five measures of quality of care: concern and empathy for the patient, professional treatment, response to treatment, educated use of resources, and patients’ sense of security. Results: Significant differences were found between nurses’ perceptions of telenursing and face-to-face nursing. The quality of face-to-face nursing was perceived as more positive than that of telenursing, in general, as were the individual measures of care quality such as providing professional treatment, response to treatment, and patients’ sense of security. Conclusions: Although telemedicine has increased significantly in recent years, nursing staff still perceived the quality of care and follow-up of face-to-face nursing as more positive. It is important to continue to monitor nurses’ perceptions and attitudes towards the strengths of these two treatment methods, not only in crisis situations, but among wider populations, and to investigate the factors that could influence these perceptions.
Relationship between Depression and Anxiety during Pregnancy, Delivery-Related Outcomes, and Healthcare Utilization in Michigan Medicaid, 2012–2021
Authors: Zivin Kara , Zhang Xiaosong , Tilea Anca , Clark Sarah J. , Hall Stephanie V.
Published: November 2023

To evaluate associations between depression and/or anxiety disorders during pregnancy (DAP), delivery-related outcomes, and healthcare utilization among individuals with Michigan Medicaid-funded deliveries. We conducted a retrospective delivery-level analysis comparing delivery-related outcomes and healthcare utilization among individuals with and without DAP between January 2012 and September 2021. We used generalized estimating equation models assessing cesarean and preterm delivery; 30-day readmission after delivery; severe maternal morbidity within 42 days of delivery; and ambulatory, inpatient, emergency department or observation (ED), psychotherapy, or substance use disorders (SUD) visits during pregnancy. We adjusted models for age, race/ethnicity, urbanicity, federal poverty level, and obstetric comorbidities. Among 170,002 Michigan Medicaid enrollees with 218,890 deliveries, 29,665 (13.6%) had diagnoses of DAP. Compared to those without DAP, individuals with DAP were more often White, rural dwelling, had lower income, and had more comorbidities. In adjusted models, deliveries with DAP had higher odds of cesarean and preterm delivery OR = 1.02, 95% CI: [1.00, 1.05] and OR = 1.15, 95% CI: [1.11, 1.19] respectively), readmission within 30 days postpartum (OR = 1.14, 95% CI: [1.07, 1.22]), SMM within 42 days (OR = 1.27, 95% CI: [1.18, 1.38]), and utilization compared to those without DAP diagnoses (ambulatory: OR = 7.75, 95% CI: [6.75, 8.88], inpatient: OR = 1.13, 95% CI: [1.11, 1.15], ED: OR = 1.86, 95% CI: [1.80, 1.92], psychotherapy: OR = 172.8, 95% CI: [160.10, 186.58], and SUD: OR = 5.6, 95% CI: [5.37, 5.85]). Among delivering individuals in Michigan Medicaid, DAP had significant associations with adverse delivery-related outcomes and greater healthcare use. Early detection and intervention to address mental illness during pregnancy may help mitigate burdens of these complex yet treatable disorders.
Towards a Physiological Modeling of Sweet Cherry Blossom
Authors: Chmielewski Frank-M. , Götz Klaus-Peter
Published: November 2023

For several years, there has been a need in phenological modeling to better account for physiological processes during the winter dormancy of woody plants, which is here addressed to the sweet cherry cultivar ‘Summit’ (Prunus avium L.). This study compares three sequential phenology models (M1–M3) for the beginning of ‘Summit’ blossom in the experimental sweet cherry orchard in Berlin-Dahlem (Germany) between 2011/12–2019/20 (model development) and 2020/21–2022/23 (model validation). M1 represents an inverse modeling approach where the chilling and forcing requirements of ‘Summit’ were optimized solely from observed flowering data. In contrast, M2 and M3 are more physiologically based as they already incorporate biological knowledge, so that the model parameters were calculated directly within the specified developmental phases. Here, M2 is a two-phase model that considers experimental data for the date of endodormancy release (t1) of nine years (2011/12–2019/20) to calculate the chilling and forcing requirements. Finally, M3 is a newly developed three-phase model that additionally includes the onset of ontogenetic development (t1*) and the abscisic acid (ABA) content of ‘Summit’ flower buds during the ecodormancy phase (t1 − t1*). The results indicate that the inclusion of ABA-related heat weighting during ecodormancy significantly improves the performance of M3 compared to M1 and M2. While M1 gives satisfactory results in terms of fit and validation, it is considered physiologically unacceptable as it greatly overestimates the chilling requirement of ‘Summit’ by ignoring the ecodormancy phase. M2 accumulates too much heat during ecodormancy as it does not include control by the bud ABA content. The results highlight the need for parameters such as t1, t1*, and the bud ABA content for the physiological modeling of ‘Summit’ blossom. To the best of our knowledge, this is the first study to provide a pathway towards a physiologically based modeling approach.
Germline CSF3R Variant in Chronic Myelomonocytic Leukemia: Linking Genetic Predisposition to Uncommon Hemorrhagic Symptoms
Authors: Bochicchio Maria Teresa , Micucci Giorgia , Asioli Silvia , Ghetti Martina , Simonetti Giorgia , Lucchesi Alessandro
Published: November 2023

Chronic myelomonocytic leukemia (CMML) is a hematological neoplasm characterized by monocytosis, splenomegaly, thrombocytopenia, and anemia. Moreover, it is associated with SRSF2 mutations and, rarely, with CSF3R variants. We present the case of an 84-year-old patient with persistent anemia and monocytosis. Due to the presence of dysmorphic granulocytes, monocyte atypia, and myeloid precursors in the peripheral blood cells, the patient was subjected to a bone marrow examination. The diagnosis was consistent with CMML type 2. The Hemocoagulative test showed an increase in fibrinolysis markers. Next-generation targeted sequencing showed TET2 and SRSF2 mutations, along with an unexpected CSF3R germline missense variant, rarely encountered in CMML. The patient started Azacitidine treatment and achieved normal hemostatic process values. In conclusion, we identified a heterozygous germline mutation that, together with TET2 and SRSF2 variants, was responsible for the hemorrhagic manifestation.
Responsiveness of Candidate Genes on CoPv01CDRK/PhgPv01CDRK Loci in Common Bean Challenged by Anthracnose and Angular Leaf Spot Pathogens
Authors: Lovatto Maike , Gonçalves-Vidigal Maria Celeste , Vaz Bisneta Mariana , Calvi Alexandre Catto , Mazucheli Josmar , Vidigal Filho Pedro Soares , Miranda Eduardo Gomes Rosa , Melotto Maeli
Published: November 2023

Anthracnose (ANT) and angular leaf spot (ALS) are significant diseases in common bean, leading to considerable yield losses under specific environmental conditions. The California Dark Red Kidney (CDRK) bean cultivar is known for its resistance to multiple races of both pathogens. Previous studies have identified the CoPv01CDRK/PhgPv01CDRK resistance loci on chromosome Pv01. Here, we evaluated the expression levels of ten candidate genes near the CoPv01CDRK/PhgPv01CDRK loci and plant defense genes using quantitative real-time PCR in CDRK cultivar inoculated with races 73 of Colletotrichum lindemuthianum and 63-39 of Pseudocercospora griseola. Gene expression analysis revealed that the Phvul.001G246300 gene exhibited the most elevated levels, showing remarkable 7.8-fold and 8.5-fold increases for ANT and ALS, respectively. The Phvul.001G246300 gene encodes an abscisic acid (ABA) receptor with pyrabactin resistance, PYR1-like (PYL) protein, which plays a central role in the crosstalk between ABA and jasmonic acid responses. Interestingly, our results also showed that the other defense genes were initially activated. These findings provide critical insights into the molecular mechanisms underlying plant defense against these diseases and could contribute to the development of more effective disease management strategies in the future.
Pre-Emptive Use of Rituximab in Epstein–Barr Virus Reactivation: Incidence, Predictive Factors, Monitoring, and Outcomes
Authors: Papalexandri Apostolia , Gavriilaki Eleni , Vardi Anna , Kotsiou Nikolaos , Demosthenous Christos , Constantinou Natassa , Touloumenidou Tasoula , Zerva Panagiota , Kika Fotini , Iskas Michalis , Batsis Ioannis , Mallouri Despina , Yannaki Evangelia , Anagnostopoulos Achilles , Sakellari Ioanna
Published: November 2023

Post-transplant lymphoproliferative disease (PTLD) is a fatal complication of hematopoietic cell transplantation (HCT) associated with the Epstein–Barr virus (EBV). Multiple factors such as transplant type, graft-versus-host disease (GVHD), human leukocyte antigens (HLA) mismatch, patient age, and T-lymphocyte-depleting treatments increase the risk of PTLD. EBV reactivation in hematopoietic cell transplant recipients is monitored through periodic quantitative polymerase chain reaction (Q-PCR) tests. However, substantial uncertainty persists regarding the clinically significant EBV levels for these patients. Guidelines recommend initiating EBV monitoring no later than four weeks post-HCT and conducting it weekly. Pre-emptive therapies, such as the reduction of immunosuppressive therapy and the administration of rituximab to treat EBV viral loads are also suggested. In this study, we investigated the occurrence of EBV-PTLD in 546 HCT recipients, focusing on the clinical manifestations and risk factors associated with the disease. We managed to identify 67,150 viral genomic copies/mL as the cutoff point for predicting PTLD, with 80% sensitivity and specificity. Among our cohort, only 1% of the patients presented PTLD. Anti-thymocyte globulin (ATG) and GVHD were independently associated with lower survival rates and higher treatment-related mortality. According to our findings, prophylactic measures including regular monitoring, pre-emptive therapy, and supportive treatment against infections can be effective in preventing EBV-related complications. This study also recommends conducting EBV monitoring at regular intervals, initiating pre-emptive therapy when viral load increases, and identifying factors that increase the risk of PTLD. Our study stresses the importance of frequent and careful follow-ups of post-transplant complications and early intervention in order to improve survival rates and reduce mortality.
Skeletal Morphogenesis and Anomalies in Gilthead Seabream: A Comprehensive Review
Authors: Mhalhel Kamel , Levanti Maria , Abbate Francesco , Laurà Rosaria , Guerrera Maria Cristina , Aragona Marialuisa , Porcino Caterina , Pansera Lidia , Sicari Mirea , Cometa Marzio , Briglia Marilena , Germanà Antonino , Montalbano Giuseppe
Published: November 2023

The gilthead seabream, one of the most important species in Mediterranean aquaculture, with an increasing status of exploitation in terms of production volume and aquafarming technologies, has become an important research topic over the years. The accumulation of knowledge from several studies conducted during recent decades on their functional and biological characteristics has significantly improved their aquacultural aspects, namely their reproductive success, survival, and growth. Despite the remarkable progress in the aquaculture industry, hatchery conditions are still far from ideal, resulting in frequent abnormalities at the beginning of intensive culture, entailing significant economic losses. Those deformities are induced during the embryonic and post-embryonic periods of life, and their development is still poorly understood. In the present review, we created a comprehensive synthesis that covers the various aspects of skeletal morphogenesis and anomalies in the gilthead seabream, highlighting the genetic, environmental, and nutritional factors contributing to bone deformities and emphasized the potential of the gilthead seabream as a model organism for understanding bone morphogenesis in both aquaculture and translational biological research. This review article addresses the existing lack in the literature regarding gilthead seabream bone deformities, as there are currently no comprehensive reviews on this subject.
Effect of a Flavonoid Combination of Apigenin and Epigallocatechin-3-Gallate on Alleviating Intestinal Inflammation in Experimental Colitis Models
Authors: Li Mingrui , Weigmann Benno
Published: November 2023

Inflammatory bowel disease (IBD) is an autoimmune disease that leads to severe bowel symptoms and complications. Currently, there is no effective treatment, and the exact cause of IBD remains unclear. In the last decades, numerous studies have confirmed that flavonoids can have a positive impact on the treatment of IBD. Therefore, this study investigated the protective effect of a flavonoid combination of apigenin and epigallocatechin-3-gallate (EGCG) on IBD. In vitro studies in which Caco-2 cell monolayers were incubated with different concentrations of flavonoids found that the flavonoid-treated group exhibited increased transepithelial electrical resistance (TEER) at high concentrations, indicating a protective effect on the barrier function of the intestinal epithelium. In vivo studies showed that flavonoids significantly attenuated inflammatory levels in both chronic and acute hapten-mediated experimental colitis models in a time- and dose-dependent manner. In addition, the activity of myeloperoxidase (MPO) and the level of proinflammatory cytokines in the colon tissue were significantly reduced. Interestingly, the levels of anti-inflammatory cytokines were also dramatically increased. Finally, flavonoids were found to positively modulate the composition of the gut microbiota in the colon. Therefore, a combination of flavonoids could be a promising therapeutic agent for the future adjunctive treatment of IBD.
Animal Models for the Study of Gaucher Disease
Authors: Cabasso Or , Kuppuramalingam Aparna , Lelieveld Lindsey , Van der Lienden Martijn , Boot Rolf , Aerts Johannes M. , Horowitz Mia
Published: November 2023

In Gaucher disease (GD), a relatively common sphingolipidosis, the mutant lysosomal enzyme acid β-glucocerebrosidase (GCase), encoded by the GBA1 gene, fails to properly hydrolyze the sphingolipid glucosylceramide (GlcCer) in lysosomes, particularly of tissue macrophages. As a result, GlcCer accumulates, which, to a certain extent, is converted to its deacylated form, glucosylsphingosine (GlcSph), by lysosomal acid ceramidase. The inability of mutant GCase to degrade GlcSph further promotes its accumulation. The amount of mutant GCase in lysosomes depends on the amount of mutant ER enzyme that shuttles to them. In the case of many mutant GCase forms, the enzyme is largely misfolded in the ER. Only a fraction correctly folds and is subsequently trafficked to the lysosomes, while the rest of the misfolded mutant GCase protein undergoes ER-associated degradation (ERAD). The retention of misfolded mutant GCase in the ER induces ER stress, which evokes a stress response known as the unfolded protein response (UPR). GD is remarkably heterogeneous in clinical manifestation, including the variant without CNS involvement (type 1), and acute and subacute neuronopathic variants (types 2 and 3). The present review discusses animal models developed to study the molecular and cellular mechanisms underlying GD.
Exposure to Chlorpyrifos Alters Proliferation, Differentiation and Fatty Acid Uptake in 3T3-L1 Cells
Authors: Czajka Magdalena , Sawicki Krzysztof , Matysiak-Kucharek Magdalena , Kruszewski Marcin , Kurzepa Jacek , Wojtyła-Buciora Paulina , Kapka-Skrzypczak Lucyna
Published: November 2023

Organophosphorus pesticides (OPs) are important factors in the etiology of many diseases, including obesity and type 2 diabetes mellitus. The aim of this study was to investigate the effect of a representative of OPs, chlorpyrifos (CPF), on viability, proliferation, differentiation, and fatty acid uptake in 3T3-L1 cells. The effect of CPF exposure on preadipocyte proliferation was examined by the MTT, NR, and BrdU assays. The impact of CPF exposure on the differentiation of preadipocytes into mature adipocytes was evaluated by Oil Red O staining and RT-qPCR. The effect of CPF on free fatty acid uptake in adipocytes was assessed with the fluorescent dye BODIPY. Our experiments demonstrated that exposure to CPF decreased the viability of 3T3-L1 cells; however, it was increased when the cells were exposed to low concentrations of the pesticide. Exposure to CPF inhibited the proliferation and differentiation of 3T3-L1 preadipocytes. CPF exposure resulted in decreased lipid accumulation, accompanied by down-regulation of the two key transcription factors in adipogenesis: C/EBPα and PPARγ. Exposure to CPF increased basal free fatty acid uptake in fully differentiated adipocytes but decreased this uptake when CPF was added during the differentiation process. Increased free fatty acid accumulation in fully differentiated adipocytes may suggest that CPF leads to adipocyte hypertrophy, one of the mechanisms leading to obesity, particularly in adults. It can therefore be concluded that CPF may disturb the activity of preadipocytes and adipocytes, although the role of this pesticide in the development of obesity requires further research.
Circulating Extracellular Vesicle-Derived microRNAs as Novel Diagnostic and Prognostic Biomarkers for Non-Viral-Related Hepatocellular Carcinoma
Authors: Boonkaew Bootsakorn , Satthawiwat Nantawat , Pinjaroen Nutcha , Chuaypen Natthaya , Tangkijvanich Pisit
Published: November 2023

Extracellular vesicle-derived microRNAs (EV-miRNAs) are promising circulating biomarkers for chronic liver disease. In this study, we explored the potential significance of plasma EV-miRNAs in non-hepatitis B-, non-hepatitis C-related HCC (NBNC-HCC). We compared, using the NanoString method, plasma EV-miRNA profiles between NBNC-HCC and control groups including patients with non-alcoholic fatty liver disease (NAFLD) and healthy controls. The differentially expressed EV-miRNAs were validated in another set of plasma samples by qRT-PCR. A total of 66 significantly differentially expressed EV-miRNAs between the HCC and the control groups were identified in the discovery set. In the validation cohort, including plasma samples of 70 NBNC-HCC patients, 70 NAFLD patients, and 35 healthy controls, 5 plasma EV-miRNAs were significantly elevated in HCC, which included miR-19-3p, miR-16-5p, miR-223-3p, miR-30d-5p, and miR-451a. These miRNAs were found to participate in several cancer-related signaling pathways based on bioinformatic analysis. Among them, EV-miR-19-3p exhibited the best diagnostic performance and displayed a high sensitivity for detecting alpha-fetoprotein-negative HCC and early-stage HCC. In multivariate analysis, a high EV-miR-19-3p level was demonstrated as an independently unfavorable predictor of overall survival in patients with NBNC-HCC. In conclusion, our data have indicated, for the first time, that EV-miR-19-3p could serve as a novel circulating biomarker for the diagnosis and prognosis of NBNC-HCC.
Effect of Flavonoids on MCP-1 Expression in Human Coronary Artery Endothelial Cells and Impact on MCP-1-Dependent Migration of Human Monocytes
Authors: Brüser Lea , Teichmann Elisa , Hinz Burkhard
Published: November 2023

The monocyte chemoattractant protein-1 (MCP-1), also known as chemokine (CC motif) ligand 2 (CCL2), is involved in the formation, progression, and destabilization of atheromatous plaques. Flavonoids, found in fruits and vegetables, have been associated with various health-promoting properties, including antioxidant, anti-inflammatory, and cardioprotective effects. In the present study, the flavonoids quercetin, kaempferol, and luteolin, but not cannflavin A, were shown to substantially inhibit interleukin (IL)-1β-induced MCP-1 mRNA and protein expression in human coronary artery endothelial cells (HCAEC). At the functional level, conditioned medium (CM) from IL-1β-stimulated HCAEC caused an increase in the migration of THP-1 monocytes compared with CM from unstimulated HCAEC. However, this induction was suppressed when IL-1β-treated HCAEC were coincubated with quercetin, kaempferol, or luteolin. The functional importance of MCP-1 in IL-1β-induced monocyte migration was supported by experiments showing that neutralization of MCP-1 in the CM of IL-1β-treated HCAEC led to a significant inhibition of migration. In addition, a concentration-dependent induction of monocyte migration in the presence of recombinant MCP-1 was demonstrated. Collectively, the flavonoids quercetin, kaempferol, and luteolin were found to exert potential antiatherogenic effects in HCAEC, challenging further studies with these compounds.
HCV and HCC Tango—Deciphering the Intricate Dance of Disease: A Review Article
Authors: Milosevic Ivana , Todorovic Nevena , Filipovic Ana , Simic Jelena , Markovic Marko , Stevanovic Olja , Malinic Jovan , Katanic Natasa , Mitrovic Nikola , Nikolic Natasa
Published: November 2023

Hepatitis C virus (HCV) is a major cause of hepatocellular carcinoma (HCC) accounting for around one-third of all HCC cases. Prolonged inflammation in chronic hepatitis C (CHC), maintained through a variety of pro- and anti-inflammatory mediators, is one of the aspects of carcinogenesis, followed by mitochondrial dysfunction and oxidative stress. Immune response dysfunction including the innate and adaptive immunity also plays a role in the development, as well as in the recurrence of HCC after treatment. Some of the tumor suppressor genes inhibited by the HCV proteins are p53, p73, and retinoblastoma 1. Mutations in the telomerase reverse transcriptase promoter and the oncogene catenin beta 1 are two more important carcinogenic signaling pathways in HCC associated with HCV. Furthermore, in HCV-related HCC, numerous tumor suppressor and seven oncogenic genes are dysregulated by epigenetic changes. Epigenetic regulation of gene expression is considered as a lasting “epigenetic memory”, suggesting that HCV-induced changes persist and are associated with liver carcinogenesis even after cure. Epigenetic changes and immune response dysfunction are recognized targets for potential therapy of HCC.
Genome-Wide Identification of PAP1 Direct Targets in Regulating Seed Anthocyanin Biosynthesis in Arabidopsis
Authors: Guo Yuan , Li Dong , Liu Tiantian , Li Yuxin , Liu Jiajia , He Mingyuan , Cui Xiaohui , Liu Zijin , Chen Mingxun
Published: November 2023

Anthocyanins are widespread water-soluble pigments in the plant kingdom. Anthocyanin accumulation is activated by the MYB-bHLH-WD40 (MBW) protein complex. In Arabidopsis, the R2R3-MYB transcription factor PAP1 activates anthocyanin biosynthesis. While prior research primarily focused on seedlings, seeds received limited attention. This study explores PAP1’s genome-wide target genes in anthocyanin biosynthesis in seeds. Our findings confirm that PAP1 is a positive regulator of anthocyanin biosynthesis in Arabidopsis seeds. PAP1 significantly increased anthocyanin content in developing and mature seeds in Arabidopsis. Transcriptome analysis at 12 days after pollination reveals the upregulation of numerous genes involved in anthocyanin accumulation in 35S:PAP1 developing seeds. Chromatin immunoprecipitation and dual luciferase reporter assays demonstrate PAP1’s direct promotion of ten key genes and indirect upregulation of TT8, TTG1, and eight key genes during seed maturation, thus enhancing seed anthocyanin accumulation. These findings enhance our understanding of PAP1’s novel role in regulating anthocyanin accumulation in Arabidopsis seeds.
Osa-miR11117 Targets OsPAO4 to Regulate Rice Immunity against the Blast Fungus Magnaporthe oryzae
Authors: Gao Shang , Hou Yan , Huang Qiwei , Wu Pengzhi , Han Zhikai , Wei Danhong , Xie Huabin , Gu Fengwei , Chen Chun , Wang Jiafeng
Published: November 2023

The intricate regulatory process governing rice immunity against the blast fungus Magnaporthe oryzae remains a central focus in plant–pathogen interactions. In this study, we investigated the important role of Osa-miR11117, an intergenic microRNA, in regulating rice defense mechanisms. Stem-loop qRT-PCR analysis showed that Osa-miR11117 is responsive to M. oryzae infection, and overexpression of Osa-miR11117 compromises blast resistance. Green fluorescent protein (GFP)-based reporter assay indicated OsPAO4 is one direct target of Osa-miR11117. Furthermore, qRT-PCR analysis showed that OsPAO4 reacts to M. oryzae infection and polyamine (PA) treatment. In addition, OsPAO4 regulates rice resistance to M. oryzae through the regulation of PA accumulation and the expression of the ethylene (ETH) signaling genes. Taken together, these results suggest that Osa-miR11117 is targeting OsPAO4 to regulate blast resistance by adjusting PA metabolism and ETH signaling pathways.
The Modification of the Illumina® CovidSeq™ Workflow for RSV Genomic Surveillance: The Genetic Variability of RSV during the 2022–2023 Season in Northwest Spain
Authors: Davina-Nunez Carlos , Perez-Castro Sonia , Cabrera-Alvargonzalez Jorge Julio , Montano-Barrientos Jhon , Godoy-Diz Montse , Regueiro Benito
Published: November 2023

There is growing interest in the molecular surveillance of the Respiratory Syncytial Virus and the monitorization of emerging mutations that could impair the efficacy of antiviral prophylaxis and treatments. A simple, scalable protocol for viral nucleic acid enrichment could improve the surveillance of RSV. We developed a protocol for RSV-A and B amplification based on the Illumina CovidSeq workflow using an RSV primer panel. A total of 135 viral genomes were sequenced from nasopharyngeal samples through the optimization steps of this panel, while an additional 15 samples were used to test the final version. Full coverage of the G gene and over 95% of the coverage of the F gene, the target of the available RSV antivirals or monoclonal antibodies, were obtained. The F:K68N mutation, associated with decreased nirsevimab activity, was detected in our facility. Additionally, phylogenetic analysis showed several sublineages in the 2022–2023 influenza season in Europe. Our protocol allows for a simple and scalable simultaneous amplification of the RSV-A and B whole genome, increasing the yield of RSV sequencing and reducing costs. Its application would allow the world to be ready for the detection of arising mutations in relation to the widespread use of nirsevimab for RSV prevention.
Generation of a Novel SORT1×HER2 Bispecific Antibody–Drug Conjugate Targeting HER2-Low-Expression Tumor
Authors: Zhuang Weiliang , Zhang Wei , Wang Lei , Xie Liping , Feng Jun , Zhang Baohong , Hu Youjia
Published: November 2023

Human epidermal growth factor receptor 2 (HER2) is considered an ideal antibody–drug conjugate (ADC) target because the gene is overexpressed in many tumors compared to normal tissues. Multiple anti-HER2 ADCs conjugated with different toxic payloads bring benefits to patients with high HER2 expression. However, HER2-targeted ADC technology needs further optimization to improve its effect for the treatment of patients with low HER2 expression. We hypothesized that bispecific antibody–drug conjugate (bsADC) targeting HER2 and Sortilin-1 (SORT1) would overcome this limitation. SORT1 is a suitable target for pairing with HER2 to generate a bispecific antibody (BsAb) since the gene is co-expressed with HER2 in tumors and possesses rapid internalization. We developed a BsAb (bsSORT1×HER2) that exhibited strong binding and internalization activity on HER2-low-expression tumor cells and facilitated higher HER2 degradation. The bsSORT1×HER2 was further conjugated with DXd to generate a bsADC (bsSORT1×HER2-DXd) that showed strong cytotoxicity on HER2-low-expression tumor cells and antitumor efficacy in an MDA-MB-231 xenograft mice model. These results demonstrated that employment of a SORT1×HER2-targeted bsADC may be promising to improve the antitumor efficacy of HER2-targeted ADC for the treatment of tumors with low HER2 expression.
The VEST External Support for Saphenous Vein Grafts in Coronary Surgery: A Review of Randomized Clinical Trials
Authors: Soletti Giovanni Jr. , Dell’Aquila Michele , Harik Lamia , Cancelli Gianmarco , Alzghari Talal , Perezgrovas-Olaria Roberto , Dimagli Arnaldo , An Kevin R. , Leith Jordan , Rossi Camilla Sofia , Barile Christopher F. , Demetres Michelle , Lau Christopher , Girardi Leonard N. , Gaudino Mario
Published: November 2023

Saphenous vein grafts (SVGs) are the most frequently used conduits in coronary artery bypass grafting (CABG), but their higher rate of occlusion compared to arterial conduits remains a concern. Previous studies have shown that SVG failure is mainly driven by intimal hyperplasia, an adaptative response to higher pressures of the arterial circulation. The VESTTM device (Vascular Graft Solutions, Tel Aviv, Israel), an external support designed to mitigate intimal hyperplasia in SVGs, has been tested in few clinical trials (RCTs). Herein, we descriptively evaluated the randomized evidence on the VEST device.
Lesion Detection in Optical Coherence Tomography with Transformer-Enhanced Detector
Authors: Ahmed Hanya , Zhang Qianni , Wong Ferranti , Donnan Robert , Alomainy Akram
Published: November 2023

Optical coherence tomography (OCT) is an emerging imaging tool in healthcare with common applications in ophthalmology for the detection of retinal diseases and in dentistry for the early detection of tooth decay. Speckle noise is ubiquitous in OCT images, which can hinder diagnosis by clinicians. In this paper, a region-based, deep learning framework for the detection of anomalies is proposed for OCT-acquired images. The core of the framework is Transformer-Enhanced Detection (TED), which includes attention gates (AGs) to ensure focus is placed on the foreground while identifying and removing noise artifacts as anomalies. TED was designed to detect the different types of anomalies commonly present in OCT images for diagnostic purposes and thus aid clinical interpretation. Extensive quantitative evaluations were performed to measure the performance of TED against current, widely known, deep learning detection algorithms. Three different datasets were tested: two dental and one CT (hosting scans of lung nodules, livers, etc.). The results showed that the approach verifiably detected tooth decay and numerous lesions across two modalities, achieving superior performance compared to several well-known algorithms. The proposed method improved the accuracy of detection by 16–22% and the Intersection over Union (IOU) by 10% for both dentistry datasets. For the CT dataset, the performance metrics were similarly improved by 9% and 20%, respectively.
Effect of Salt Mist Ageing on the Physical and Mechanical Properties of Two Ignimbrites from the Canary Islands (Spain)
Authors: Valido José A. , Cáceres José M. , Freire-Lista D. M. , Sousa Luís M. O.
Published: November 2023

The aim of this research work is to study the effect of salt mist ageing on the physico-mechanical properties of two ignimbrites from the Canary Islands (Spain). Due to their insular nature and extensive coastlines, these regions are highly susceptible to atmospheric salt aerosols, which is a significant weathering agent for building materials. The ignimbrites analysed are marketed under the names “Azul Lomo Tomás de León” and “Marrón de Abades” and are widely used as building stones. Petrographic, mineralogical and chemical properties were obtained via polarisation microscopy, X-ray diffraction and X-ray fluorescence. The samples were subjected to 60 cycles of a salt mist environment, following relevant European standards. Scanning electron microscopy images of the specimens were analysed along with physical properties, including apparent density, open porosity, water absorption, colourimetry and ultrasound propagation velocity, initially and after every 20 cycles, allowing us to assess their evolution during the ageing test. Mechanical properties were evaluated using uniaxial compressive strength and flexural strength under concentrated load tests on two groups of specimens: one unaged and the other subjected to 60 ageing cycles. With the exception of apparent density, the other properties show a decrease between 5% and 30%. However, open porosity increases and is one of the properties most affected by the ageing test.
Electrospun Nanofibers: Shaping the Future of Controlled and Responsive Drug Delivery
Authors: Wildy Michael , Lu Ping
Published: November 2023

Electrospun nanofibers for drug delivery systems (DDS) introduce a revolutionary means of administering pharmaceuticals, holding promise for both improved drug efficacy and reduced side effects. These biopolymer nanofiber membranes, distinguished by their high surface area-to-volume ratio, biocompatibility, and biodegradability, are ideally suited for pharmaceutical and biomedical applications. One of their standout attributes is the capability to offer the controlled release of the active pharmaceutical ingredient (API), allowing custom-tailored release profiles to address specific diseases and administration routes. Moreover, stimuli-responsive electrospun DDS can adapt to conditions at the drug target, enhancing the precision and selectivity of drug delivery. Such localized API delivery paves the way for superior therapeutic efficiency while diminishing the risk of side effects and systemic toxicity. Electrospun nanofibers can foster better patient compliance and enhanced clinical outcomes by amplifying the therapeutic efficiency of routinely prescribed medications. This review delves into the design principles and techniques central to achieving controlled API release using electrospun membranes. The advanced drug release mechanisms of electrospun DDS highlighted in this review illustrate their versatility and potential to improve the efficacy of medical treatments.
Magnetic Nanoparticles with Fe-N and Fe-C Cores and Carbon Shells Synthesized at High Pressures
Authors: Bagramov Rustem , Filonenko Vladimir , Zibrov Igor , Skryleva Elena , Kulnitskiy Boris , Blank Vladimir , Khabashesku Valery
Published: November 2023

Nanoparticles of iron carbides and nitrides enclosed in graphite shells were obtained at 2 ÷ 8 GPa pressures and temperatures of around 800 °C from ferrocene and ferrocene–melamine mixture. The average core–shell particle size was below 60 nm. The graphite-like shells over the iron nitride cores were built of concentric graphene layers packed in a rhombohedral shape. It was found that at a pressure of 4 GPa and temperature of 800 °C, the stability of the nanoscale phases increases in a Fe7C3 -> Fe3C -> Fe3N1+x sequence and at 8 GPa in a Fe3C -> Fe7C3 -> Fe3N1+x sequence. At pressures of 2 ÷ 8 GPa and temperatures up to 1600 °C, iron nitride Fe3N1+x is more stable than iron carbides. At 8 GPa and 1600 °C, the average particle size of iron nitride increased to 0.5 ÷ 1 μm, while simultaneously formed free carbon particles had the shape of graphite discs with a size of 1 ÷ 2 μm. Structural refinement of the iron nitride using the Rietveld method gave the best result for the space group P6322. The refined composition of the samples obtained from a mixture of ferrocene and melamine at 8 GPa/800 °C corresponded to Fe3N1.208, and at 8 GPa/1650 °C to Fe3N1.259. The iron nitride core–shell nanoparticles exhibited magnetic behavior. Specific magnetization at 7.5 kOe of pure Fe3N1.208 was estimated to be 70 emu/g. Compared to other methods, the high-pressure method allows easy synthesis of the iron nitride cores inside pure carbon shells and control of the particle size. And in general, pressure is a good tool for modifying the phase and chemical composition of the iron-containing cores.