bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Hemodynamic Visualization in Cerebral Arteries: Can Magnetic Resonance Imaging Replace Computational Fluid Dynamics?
Authors: Ngo Minh Tri , Lee Ui Yun , Ha Hojin , Jin Ning , Chung Gyung Ho , Kwak Yeong Gon , Jung Jinmu , Kwak Hyo Sung
Published: March 2021

A multimodality approach was applied using four-dimensional flow magnetic resonance imaging (4D flow MRI), time-of-flight magnetic resonance angiography (TOF-MRA) signal intensity gradient (SIG), and computational fluid dynamics (CFD) to investigate the 3D blood flow characteristics and wall shear stress (WSS) of the cerebral arteries. TOF-MRA and 4D flow MRI were performed on the major cerebral arteries in 16 healthy volunteers (mean age 34.7 ± 7.6 years). The flow rate measured with 4D flow MRI in the internal carotid artery, middle cerebral artery, and anterior cerebral artery were 3.8, 2.5, and 1.2 mL/s, respectively. The 3D blood flow pattern obtained through CFD and 4D flow MRI on the cerebral arteries showed reasonable consensus. CFD delivered much greater resolution than 4D flow MRI. TOF-MRA SIG and CFD WSS of the major cerebral arteries showed reasonable consensus with the locations where the WSS was relatively high. However, the visualizations were very different between TOF-MRA SIG and CFD WSS at the internal carotid artery bifurcations, the anterior cerebral arteries, and the anterior communicating arteries. 4D flow MRI, TOF-MRA SIG, and CFD are complementary methods that can provide additional insight into the hemodynamics of the human cerebral artery.
Initial Experience of the Levodopa–Entacapone–Carbidopa Intestinal Gel in Clinical Practice
Authors: Öthman Mezin , Widman Erik , Nygren Ingela , Nyholm Dag
Published: March 2021

Patients in fluctuating stages of Parkinson’s disease (PD) require device-aided treatments. Continuous infusion of levodopa–carbidopa intestinal gel (LCIG) is a well-proven option in clinical practice. We now report the first clinical experience of levodopa–entacapone–carbidopa intestinal gel (LECIG) therapy. An observational study of the first patients to start LECIG in our clinic was performed. Twenty-four patients (11 females, 13 males) were included. The median age was 71.5 years, and the median duration since PD diagnosis was 15.5 years. The median treatment duration was 305 days. Median doses were: 6.0 mL as morning dose, 2.5 mL/h as infusion rate, and 1.0 mL as extra dose. Half of the patients were switched directly from LCIG. These patients express improvements in the size and weight of the pump. Furthermore, most of them considered the new pump to be improved regarding user-friendliness. Six patients discontinued LECIG, three due to diarrhea, one due to hallucinations and two deceased (one cardiac arrest and one COVID-19). LECIG has shown to be possible to use in patients with PD, efficacy and safety as expected. Patients are generally happy with the size and usability of the pump, but some technical improvements of the software are warranted, as well as larger, prospective studies.
An Approach for Stego-Insider Detection Based on a Hybrid NoSQL Database
Authors: Kotenko Igor , Krasov Andrey , Ushakov Igor , Izrailov Konstantin
Published: March 2021

One of the reasons for the implementation of information security threats in organizations is the insider activity of its employees. There is a big challenge to detect stego-insiders-employees who create stego-channels to secretly receive malicious information and transfer confidential information across the organization’s perimeter. Especially presently, with great popularity of wireless sensor networks (WSNs) and Internet of Things (IoT) devices, there is a big variety of information that could be gathered and processed by stego-insiders. Consequently, the problem arises of identifying such intruders and their transmission channels. The paper proposes an approach to solving this problem. The paper provides a review of the related works in terms of insider models and methods of their identification, including techniques for handling insider attacks in WSN, as well methods of embedding and detection of stego-embeddings. This allows singling out the basic features of stego-insiders, which could be determined by their behavior in the network. In the interests of storing these attributes of user behavior, as well as storing such attributes from large-scale WSN, a hybrid NoSQL database is created based on graph and document-oriented approaches. The algorithms for determining each of the features using the NoSQL database are specified. The general scheme of stego-insider detection is also provided. To confirm the efficiency of the approach, an experiment was carried out on a real network. During the experiment, a database of user behavior was collected. Then, user behavior features were retrieved from the database using special SQL queries. The analysis of the results of SQL queries is carried out, and their applicability for determining the attribute is justified. Weak points of the approach and ways to improve them are indicated.
Understanding Urban Land Growth through a Social-Spatial Perspective
Authors: Zhang Liqin , Han Ruibo , Cao Huhua
Published: March 2021

To understand the urbanization process, it is essential to detect urban spatial growth and to study relations with social development. In this study, we take Wuhan as a case to examine urban land growth patterns and how social factors relate to the urban land evolution between 1990, 2000, and 2010. We first classify land cover using Landsat images and examine the urban growth patterns during various stages based on landscape metrics regarding the area, density, and shape. Afterwards, principal component analysis and census data are used to extract key social factors. Thirdly, we apply geographically weighted regression (GWR) to depict the link between urban land metrics and social factors. The results indicate that the urban land coalescence and diffusion simultaneously exist, for which redevelopment, infilling, and edge expansion dominate the city center, and diffusion dominates the peripheral areas. The social factors have global regression relationships with urban land areas while local spatial non-stationarity presents in the relationships with the urban land patch shape irregularities. Industrial upgrading, educational levelling up, and population aging show significant with local heterogeneities in the relationships. The simulation of the relationship provides a social-spatial perspective to understand urban land growth. The authors conclude that sustainable urban management should consider the coexistence of different urban spatial growth models and underline social transitions when examining the urban growth process. This works for cities in rapidly urbanizing countries or regions.
Beat Detection Recruits the Visual Cortex in Early Blind Subjects
Authors: Araneda Rodrigo , Silva Moura Sandra , Dricot Laurence , De Volder Anne G.
Published: March 2021

Using functional magnetic resonance imaging, here we monitored the brain activity in 12 early blind subjects and 12 blindfolded control subjects, matched for age, gender and musical experience, during a beat detection task. Subjects were required to discriminate regular (“beat”) from irregular (“no beat”) rhythmic sequences composed of sounds or vibrotactile stimulations. In both sensory modalities, the brain activity differences between the two groups involved heteromodal brain regions including parietal and frontal cortical areas and occipital brain areas, that were recruited in the early blind group only. Accordingly, early blindness induced brain plasticity changes in the cerebral pathways involved in rhythm perception, with a participation of the visually deprived occipital brain areas whatever the sensory modality for input. We conclude that the visually deprived cortex switches its input modality from vision to audition and vibrotactile sense to perform this temporal processing task, supporting the concept of a metamodal, multisensory organization of this cortex.
Exceptional Mechanical Properties and Heat Resistance of Photocurable Bismaleimide Ink for 3D Printing
Authors: Hua Wenqiang , Lin Qilang , Qu Bo , Zheng Yanyu , Liu Xiaoying , Li Wenjie , Zhao Xiaojing , Chen Shaoyun , Zhuo Dongxian
Published: March 2021

Photosensitive resins used in three-dimensional (3D) printing are characterized by high forming precision and fast processing speed; however, they often possess poor mechanical properties and heat resistance. In this study, we report a photocurable bismaleimide ink with excellent comprehensive performance for stereolithography (SLA) 3D printing. First, the main chain of bismaleimide with an amino group (BDM) was synthesized, and then, the glycidyl methacrylate was grafted to the amino group to obtain the bismaleimide oligomer with an unsaturated double bond. The oligomers were combined with reaction diluents and photo-initiators to form photocurable inks that can be used for SLA 3D printing. The viscosity and curing behavior of the inks were studied, and the mechanical properties and heat resistance were tested. The tensile strength of 3D-printed samples based on BDM inks could reach 72.6 MPa (166% of that of commercial inks), glass transition temperature could reach 155 °C (205% of that of commercial inks), and energy storage modulus was 3625 MPa at 35 °C (327% of that of commercial inks). The maximum values of T-5%, T-50%, and Tmax of the 3D samples printed by BDM inks reached 351.5, 449.6, and 451.9 °C, respectively. These photocured BDM inks can be used to produce complex structural components and models with excellent mechanical and thermal properties, such as car parts, building models, and pipes.
Erratum: Guillermo M., et al. Environmental Impact of Nanoparticles’ Application as an Emerging Technology: A Review. Materials 2021, 14, 166
Authors: Martínez Guillermo , Merinero Manuel , Pérez-Aranda María , Pérez-Soriano Eva María , Ortiz Tamara , Villamor Eduardo , Begines Belén , Alcudia Ana
Published: March 2021

The authors wish to make the following corrections to this paper [...]
Research on High-Value Utilization of Carbon Derived from Tobacco Waste in Supercapacitors
Authors: Huang Zhenrui , Qin Caiyun , Wang Jun , Cao Lin , Ma Zhuwen , Yuan Qinghua , Lin Zhidan , Zhang Peng
Published: March 2021

Large quantities of tobacco stalks residues are generated and discarded as crop waste or combusted directly every year. Thus, we need to find an appropriate way to dispose of this type of waste and recycle it. The conversion of biomass waste into electrode materials for supercapacitors is entirely in line with the concept of sustainability and green. In this paper, tobacco-stalk-based, porous activated carbon (TC) was successfully synthesized by high-temperature and high-pressure hydrothermal pre-carbonization and KOH activation. The synthesized TC had a high pore volume and a large surface area of 1875.5 m2 g−1, in which there were many mesopores and interconnected micro-/macropores. The electrochemical test demonstrated that TC-1 could reach a high specific capacitance of up to 356.4 F g−1 at a current density of 0.5 A g−1, which was carried in 6M KOH. Additionally, a symmetrical supercapacitor device was fabricated by using TC-1 as the electrode, which delivered a high energy density up to 10.4 Wh kg−1 at a power density of 300 W kg−1, and excellent long-term cycling stability (92.8% of the initial capacitance retention rate after 5000 cycles). Therefore, TC-1 is considered to be a promising candidate for high-performance supercapacitor electrode materials and is a good choice for converting tobacco biomass waste into a resource.
Synthesis of Co–Fe 1D Nanocone Array Electrodes Using Aluminum Oxide Template
Authors: Skibińska Katarzyna , Kołczyk-Siedlecka Karolina , Kutyła Dawid , Gajewska Marta , Żabiński Piotr
Published: March 2021

Porous anodic alumina oxide (AAO) obtained via two-step anodization is a material commonly used as a template for fabricating 1D nanostructures. In this work, copper and cobalt-iron 1D nanocones were obtained by an electrodeposition method using AAO templates. The templates were produced using two-step anodization in H2C2O4. The Co–Fe nanostructures are characterized by homogeneous pore distribution. The electrocatalytic activity of the produced nanomaterials was determined in 1 M NaOH using the linear sweep voltammetry (LSV) and chronopotentiometry (CP) methods. These materials can be used as catalysts in the water-splitting reaction. The sample’s active surface area was calculated and compared with bulk materials.
Growth of Semi-Polar (101¯3) AlN Film on M-Plane Sapphire with High-Temperature Nitridation by HVPE
Authors: Li Xu , Zhao Jianyun , Liu Ting , Lu Yong , Zhang Jicai
Published: March 2021

Aluminum nitride (AlN) films were grown on the m-plane sapphire by high-temperature hydride vapor phase epitaxy (HVPE). The effect of high-temperature nitridation on the quality of AlN film was studied. The high-temperature nitridation is favorable for the formation of semi-polar single (101¯3) orientation AlN film, the quality of which shows strong dependence on the nitridation temperature. The full width at half maximum of X-ray diffraction for (101¯3) AlN film was only 0.343° at the optimum nitridation temperature of 1300 °C. It is found that the nano-holes were formed on the surface of substrates by the decomposition of sapphire in the process of high-temperature nitridation, which is closely related to the quality improvement of AlN. At the critical nitridation temperature of 1300 °C, the average size of the nano-holes is about 70 nm, which is in favor of promoting the rapid coalescence of AlN micro-grains in the early stages. However, the size of nano-holes will be enlarged with the further increase of nitridation temperature, which begins to play a negative role in the coalescence of AlN grains. As a result, the grain size will be increased and extended to the epilayer, leading to the deterioration of the AlN film.
Performance Prediction of a Turbodrill Based on the Properties of the Drilling Fluid
Authors: Zhang Delong , Wang Yu , Sha Junjie , He Yuguang
Published: March 2021

High-temperature geothermal well resource exploration faces high-temperature and high-pressure environments at the bottom of the hole. The all-metal turbodrill has the advantages of high-temperature resistance and corrosion resistance and has good application prospects. Multistage hydraulic components, consisting of stators and rotors, are the key to the turbodrill. The purpose of this paper is to provide a basis for designing turbodrill blades with high-density drilling fluid under high-temperature conditions. Based on the basic equation of pseudo-fluid two-phase flow and the modified Bernoulli equation, a mathematical model for the coupling of two-phase viscous fluid flow with the turbodrill blade is established. A single-stage blade performance prediction model is proposed and extended to multi-stage blades. A Computational Fluid Dynamics (CFD) model of a 100-stage turbodrill blade channel is established, and the multi-stage blade simulation results for different fluid properties are given. The analysis confirms the influence of fluid viscosity and fluid density on the output performance of the turbodrill. The research results show that compared with the condition of clear water, the high-viscosity and high-density conditions (viscosity 16 mPa∙s, density 1.4 g/cm3) will increase the braking torque of the turbodrill by 24.2%, the peak power by 19.8%, and the pressure drop by 52.1%. The results will be beneficial to the modification of the geometry model of the blade and guide the on-site application of the turbodrill to improve drilling efficiency.
Aluminium Parts Casting Scheduling Based on Simulated Annealing
Authors: Jiménez-Martín Antonio , Mateos Alfonso , Hernández Josefa Z.
Published: March 2021

This paper focuses on the last stage of the aluminium production process in the context of Industry 4.0: schedule optimization in the casting process. Casting is one of the oldest manufacturing processes in which a liquid material is usually poured into a mold that contains a hollow cavity of the desired shape and then allowed to solidify. This is a complex scheduling problem in which several constraints, such as different maintenance processes, maximum stocks, machine breakdowns, work shifts, or the maximum number of mold changes per day, come into play. Four objective functions have to be taken into account simultaneously. We have to minimize both the unmet demand at the end of the schedule, and the delays in the injection process with regard to daily demands. Production costs, including the cost of electricity consumption in the injection process and gas consumption associated with melting furnaces, should be minimized. Finally, the total number of mold changes throughout the schedule must also be reduced to a minimum. The simulated annealing (SA) metaheuristic has been adapted to solve this complex optimization process and parameterized for application to a wide variety of aluminium making processes. SA efficiently solves the problem and provides an optimal solution in about three minutes.
Weighted Homology of Bi-Structures over Certain Discrete Valuation Rings
Authors: Bura Andrei , He Qijun , Reidys Christian
Published: March 2021

An RNA bi-structure is a pair of RNA secondary structures that are considered as arc-diagrams. We present a novel weighted homology theory for RNA bi-structures, which was obtained through the intersections of loops. The weighted homology of the intersection complex X features a new boundary operator and is formulated over a discrete valuation ring, R. We establish basic properties of the weighted complex and show how to deform it in order to eliminate any 3-simplices. We connect the simplicial homology, Hi(X), and weighted homology, Hi,R(X), in two ways: first, via chain maps, and second, via the relative homology. We compute H0,R(X) by means of a recursive contraction procedure on a weighted spanning tree and H1,R(X) via an inflation map, by which the simplicial homology of the 1-skeleton allows us to determine the weighted homology H1,R(X). The homology module H2,R(X) is naturally obtained from H2(X) via chain maps. Furthermore, we show that all weighted homology modules Hi,R(X) are trivial for i>2. The invariant factors of our structure theorems, as well as the weighted Whitehead moves facilitating the removal of filled tetrahedra, are given a combinatorial interpretation. The weighted homology of bi-structures augments the simplicial counterpart by introducing novel torsion submodules and preserving the free submodules that appear in the simplicial homology.
Metaheuristic to Optimize Computational Convergence in Convection-Diffusion and Driven-Cavity Problems
Authors: Enríquez-Urbano Juana , Cruz-Chávez Marco Antonio , Rivera-López Rafael , Cruz-Rosales Martín H. , Labrada-Nueva Yainier , Eraña-Díaz Marta Lilia
Published: March 2021

This work presents an optimization proposal to better the computational convergence time in convection-diffusion and driven-cavity problems by applying a simulated annealing (SA) metaheuristic, obtaining optimal values in relaxation factors (RF) that optimize the problem convergence during its numerical execution. These relaxation factors are tested in numerical models to accelerate their computational convergence in a shorter time. The experimental results show that the relaxation factors obtained by the SA algorithm improve the computational time of the problem convergence regardless of user experience in the initial low-quality RF proposal.
A Unified Analytical Approach to Fixed and Moving Boundary Problems for the Heat Equation
Authors: Rodrigo Marianito R. , Thamwattana Ngamta
Published: March 2021

Fixed and moving boundary problems for the one-dimensional heat equation are considered. A unified approach to solving such problems is proposed by embedding a given initial-boundary value problem into an appropriate initial value problem on the real line with arbitrary but given functions, whose solution is known. These arbitrary functions are determined by imposing that the solution of the initial value problem satisfies the given boundary conditions. Exact analytical solutions of some moving boundary problems that have not been previously obtained are provided. Moreover, examples of fixed boundary problems over semi-infinite and bounded intervals are given, thus providing an alternative approach to the usual methods of solution.
Modeling and Estimating Volatility of Day-Ahead Electricity Prices
Authors: Tashpulatov Sherzod N.
Published: March 2021

We model day-ahead electricity prices of the UK power market using skew generalized error distribution. This distribution allows us to take into account the features of asymmetry, heavy tails, and a peak higher than in normal or Student’s t distributions. The adequacy of the estimated volatility model is verified using various tests and criteria. A correctly specified volatility model can be used for analyzing the impact of reforms or other events. We find that, after the start of the COVID-19 pandemic, price level and volatility increased.
Mathematics Curriculum Reform and Its Implementation in Textbooks: Early Addition and Subtraction in Realistic Mathematics Education
Authors: Van Zanten Marc , Van den Heuvel-Panhuizen Marja
Published: March 2021

Since the late 1960s, a reform in mathematics education, which is currently known under the name Realistic Mathematics Education (RME), has been taking place in the Netherlands. Characteristic for this approach to mathematics education is that mathematics is not seen as ready-made knowledge but as an activity of the learner. Although much has been written about the big ideas and intentions of RME, and multiple RME-oriented textbooks have been published, up to now the development of this approach to mathematics education has not been thoroughly investigated. In the research reported in this article, we traced how RME has evolved over the years. The focus in our study was on early addition and subtraction in primary school. For this, we studied RME core curriculum documents and analyzed RME-oriented textbooks that have been published between the onset of RME and the present. We found that the big ideas and teaching principles of RME were clearly reflected in the learning facilitators for learning early addition and subtraction and were steadily present in curriculum documents over the years, although some were made concrete in further detail. Furthermore, we found all RME learning facilitators also to be present in all RME-oriented textbooks, though in some cases in other ways than originally intended. Our research shows the complexity of a curriculum reform process and its implementation in textbooks.
Antimicrobial Activity of Selected Medicinal Plants from a Sub-Saharan African Country against Bacterial Pathogens from Post-Operative Wound Infections
Authors: Owusu Enid , Ahorlu Martin Mensah , Afutu Emmanuel , Akumwena Amos , Asare George Awuku
Published: March 2021

Background: Globally, the application of medicinal plants in the management of acute and chronic wounds can be considered a common occurrence in most traditional medicine practices. In view of this, many plants in the tropical and subtropical regions have been screened for their wound-healing activities. Consequently, plants having antimicrobial activity against multidrug-resistant (MD-R) pathogens can be considered great assets. Therefore, this study evaluated ethanolic and aqueous extracts of five medicinal plants (Psidium guajava, Myrianthus arboreus, Alchornea cordifolia, Momordica charantia, and Justicia flava) for their antimicrobial activities against MD-R bacterial pathogens isolated from post-operative wounds; Methods: This involved the aqueous and ethanolic extraction of the selected medicinal plants. Preliminary phytochemical constituents of the plants were examined. The agar well diffusion method was then used to determine the antibacterial activity of the leaves against reference strains (Escherichia coli ATCC 25922, Salmonella typhi ATCC 19430, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923, and a Methicillin-Resistant Staphylococcus aureus strain) as well as the MDR clinical isolates (E. coli, P. aeruginosa, S. aureus and CoNS) from the wounds; Results: The preliminary phytochemical analysis of the leaves showed the presence of saponins, phenolics, and reducing sugars in almost all the plants tested. All plant extracts were observed to have some antimicrobial activity against at least one reference strain. For the clinical MDR isolates, A. cordifolia from this study showed highest inhibition to growth of all bacteria used. Activity of J. flava against S. aureus was highest as compared to that of E.coli and P. aeruginosa. Similar observation was made for M. arboreus, P. guajava and M. charantia where the highest activity was observed against S. aureus; Conclusion: This study has mainly shown that P. guajava, M. arboreus, A. cordifolia, M. charantia, and J. flava exhibits antimicrobial activities against MD-R bacterial pathogens isolated from post-operative wounds. Also, these plants has bioactive phytochemical compounds with potential medicinal values for the treatment of numerous infections. Therefore, these plants may be helpful in the management of acute and chronic wounds, especially in traditional medicine practices.
The Effect of the Oleophobicity Deterioration of a Membrane Surface on Its Rejection Capacity: A Computational Fluid Dynamics Study
Authors: Salama Amgad , Alyan Adel , El Amin Mohamed , Sun Shuyu , Zhang Tao , Zoubeik Mohamed
Published: March 2021

In this work, the effects of the deteriorating affinity-related properties of membranes due to leaching and erosion on their rejection capacity were studied via computational fluid dynamics (CFD). The function of affinity-enhancing agents is to modify the wettability state of the surface of a membrane for dispersed droplets. The wettability conditions can be identified by the contact angle a droplet makes with the surface of the membrane upon pinning. For the filtration of fluid emulsions, it is generally required that the surface of the membrane is nonwetting for the dispersed droplets such that the interfaces that are formed at the pore openings provide the membrane with a criterion for the rejection of dispersals. Since materials that make up the membrane do not necessarily possess the required affinity, it is customary to change it by adding affinity-enhancing agents to the base material forming the membrane. The bonding and stability of these materials can be compromised during the lifespan of a membrane due to leaching and erosion (in crossflow filtration), leading to a deterioration of the rejection capacity of the membrane. In order to investigate how a decrease in the contact angle can lead to the permeation of droplets that would otherwise get rejected, a CFD study was conducted. In the CFD study, a droplet was released in a crossflow field that involved a pore opening and the contact angle was considered to decrease with time as a consequence of the leaching of affinity-enhancing agents. The CFD analysis revealed that the decrease in the contact angle resulted in the droplet spreading over the surface more. Furthermore, the interface that was formed at the entrance of the pore opening flattened as the contact angle decreased, leading the interface to advance more inside the pore. The droplet continued to pass over the pore opening until the contact angle reached a certain value, at which point, the droplet became pinned at the pore opening.
gcProfileMakeR: An R Package for Automatic Classification of Constitutive and Non-Constitutive Metabolites
Authors: Perez-Sanz Fernando , Ruiz-Hernández Victoria , Terry Marta I. , Arce-Gallego Sara , Weiss Julia , Navarro Pedro J. , Egea-Cortines Marcos
Published: March 2021

Metabolomes comprise constitutive and non-constitutive metabolites produced due to physiological, genetic or environmental effects. However, finding constitutive metabolites and non-constitutive metabolites in large datasets is technically challenging. We developed gcProfileMakeR, an R package using standard Excel output files from an Agilent Chemstation GC-MS for automatic data analysis using CAS numbers. gcProfileMakeR has two filters for data preprocessing removing contaminants and low-quality peaks. The first function NormalizeWithinFiles, samples assigning retention times to CAS. The second function NormalizeBetweenFiles, reaches a consensus between files where compounds in close retention times are grouped together. The third function getGroups, establishes what is considered as Constitutive Profile, Non-constitutive by Frequency i.e., not present in all samples and Non-constitutive by Quality. Results can be plotted with the plotGroup function. We used it to analyse floral scent emissions in four snapdragon genotypes. These included a wild type, Deficiens nicotianoides and compacta affecting floral identity and RNAi:AmLHY targeting a circadian clock gene. We identified differences in scent constitutive and non-constitutive profiles as well as in timing of emission. gcProfileMakeR is a very useful tool to define constitutive and non-constitutive scent profiles. It also allows to analyse genotypes and circadian datasets to identify differing metabolites.
Trichoderma Strains and Metabolites Selectively Increase the Production of Volatile Organic Compounds (VOCs) in Olive Trees
Authors: Dini Irene , Marra Roberta , Cavallo Pierpaolo , Pironti Angela , Sepe Immacolata , Troisi Jacopo , Scala Giovanni , Lombari Pasquale , Vinale Francesco
Published: March 2021

Plants emit volatile organic compounds (VOCs) that induce metabolomic, transcriptomic, and behavioral reactions in receiver organisms, including insect pollinators and herbivores. VOCs’ composition and concentration may influence plant-insect or plant-plant interactions and affect soil microbes that may interfere in plant-plant communication. Many Trichoderma fungi act as biocontrol agents of phytopathogens and plant growth promoters. Moreover, they can stimulate plant defense mechanisms against insect pests. This study evaluated VOCs’ emission by olive trees (Olea europaea L.) when selected Trichoderma fungi or metabolites were used as soil treatments. Trichoderma harzianum strains M10, T22, and TH1, T. asperellum strain KV906, T. virens strain GV41, and their secondary metabolites harzianic acid (HA), and 6-pentyl-α-pyrone (6PP) were applied to olive trees. Charcoal cartridges were employed to adsorb olive VOCs, and gas chromatography mass spectrometry (GC-MS) analysis allowed their identification and quantification. A total of 45 volatile compounds were detected, and among these, twenty-five represented environmental pollutants and nineteen compounds were related to olive plant emission. Trichoderma strains and metabolites differentially enhanced VOCs production, affecting three biosynthetic pathways: methylerythritol 1-phosphate (MEP), lipid-signaling, and shikimate pathways. Multivariate analysis models showed a characteristic fingerprint of each plant-fungus/metabolite relationship, reflecting a different emission of VOCs by the treated plants. Specifically, strain M10 and the metabolites 6PP and HA enhanced the monoterpene syntheses by controlling the MEP pathway. Strains GV41, KV906, and the metabolite HA stimulated the hydrocarbon aldehyde formation (nonanal) by regulating the lipid-signaling pathway. Finally, Trichoderma strains GV41, M10, T22, TH1, and the metabolites HA and 6PP improve aromatic syntheses at different steps of the shikimate pathway.
Relation between Mood and the Host-Microbiome Co-Metabolite 3-Indoxylsulfate: Results from the Observational Prospective NutriNet-Santé Study
Authors: Philippe Catherine , Szabo de Edelenyi Fabien , Naudon Laurent , Druesne-Pecollo Nathalie , Hercberg Serge , Kesse-Guyot Emmanuelle , Latino-Martel Paule , Galan Pilar , Rabot Sylvie
Published: March 2021

Gut microbiota metabolizes tryptophan into indole, which can influence brain and behavior. Indeed, some oxidized derivatives of indole, formed in the liver, have neuroactive properties, and indole overproduction by the gut microbiota induces an anxio-depressive phenotype in rodents. The aim of this study was to investigate in humans whether there was a relationship between recurrent depressive symptoms and indole production by the gut microbiota. A case-control study was conducted in 45–65-year-old women, who were participants in the observational prospective NutriNet-Santé Study. Cases were defined as having two Center for Epidemiological Studies-Depression Scales (CES-D) scores ≥ 23 at a two-year interval (recurrent depressive symptoms, n = 87). Each case was matched with two controls (two CES-D <23; n = 174). Urinary excretion of 3-indoxylsulfate, the major final metabolite of indole, was used as a biomarker of indole production by the gut microbiota. Conditional logistic regression models for paired data showed a positive association between urinary 3-indoxylsulfate concentrations, grouped in tertiles, and recurrent depressive symptoms (odds ratio = 2.46, p for trend = 0.0264 in the final model adjusted for confounding factors). This association suggested that indole production by the gut microbiota may play a role in the onset of mood disorders in humans.
Sedimentary Ancient DNA (sedaDNA) Reveals Fungal Diversity and Environmental Drivers of Community Changes throughout the Holocene in the Present Boreal Lake Lielais Svētiņu (Eastern Latvia)
Authors: Talas Liisi , Stivrins Normunds , Veski Siim , Tedersoo Leho , Kisand Veljo
Published: March 2021

Fungi are ecologically important in several ecosystem processes, yet their community composition, ecophysiological roles, and responses to changing environmental factors in historical sediments are rarely studied. Here we explored ancient fungal DNA from lake Lielais Svētiņu sediment throughout the Holocene (10.5 kyr) using the ITS metabarcoding approach. Our data revealed diverse fungal taxa and smooth community changes during most of the Holocene with rapid changes occurring in the last few millennia. More precisely, plankton parasitic fungi became more diverse from the Late Holocene (2–4 kyr) which could be related to a shift towards a cooler climate. The Latest Holocene (~2 kyr) showed a distinct increase in the richness of plankton parasites, mycorrhizal, and plant pathogenic fungi which can be associated with an increased transfer rate of plant material into the lake and blooms of planktonic organisms influenced by increased, yet moderate, human impact. Thus, major community shifts in plankton parasites and mycorrhizal fungi could be utilized as potential paleo-variables that accompany host-substrate dynamics. Our work demonstrates that fungal aDNA with predicted ecophysiology and host specificity can be employed to reconstruct both aquatic and surrounding terrestrial ecosystems and to estimate the influence of environmental change.
Transcriptome Analysis on the Mechanism of Ethylicin Inhibiting Pseudomonas syringae pv. actinidiae on Kiwifruit
Authors: Liu Tao , Ren Xiaoli , Cao Guangyun , Zhou Xia , Jin Linhong
Published: March 2021

Bacterial canker disease caused by Pseudomonas syringae pv. actinidiae (Psa) is a devastating disease of kiwifruit, which is severely limiting the development of the kiwifruit industry. Ethylicin is a broad-spectrum plant biomimetic fungicide. However, its application in the control of kiwifruit bacterial canker is rarely reported, and the mechanism of ethylicin on Psa remains unknown. In this study, we investigated the effect of ethylicin on Psa in vitro and in vivo and found that ethylicin can inhibit the growth of Psa and prevent the cankering in the plant stem. Mechanism investigation indicated that ethylicin acted by limiting the movement of Psa, destroying the cell membrane of Psa, and inhibiting the formation of Psa biofilm. In addition, it was also found through transcriptomics research that ethylicin can up-regulate the expression of genes related to protein export and biofilm formation–Pseudomonas aeruginosa and down-regulate the expression of genes related to flagellar assembly in Psa. This study concluded that ethylicin can effectively inhibit Psa growth, and it could help to gain a better understanding of the mechanisms of ethylicin inhibiting Psa and provide practical data for the application of ethylicin as a highly potent agent for controlling the bacterial canker disease of kiwifruit.
New Insights into the Virulence Traits and Antibiotic Resistance of Enterococci Isolated from Diverse Probiotic Products
Authors: Deng Fengru , Chen Yunsheng , Zhou Xiaoyu , Xiao Huiying , Sun Tianyu , Deng Yiqun , Wen Jikai
Published: March 2021

The GRAS (generally recognized as safe) status of Enterococcus has not yet been authenticated, but enterococci, as probiotics, have been increasingly applied in human healthcare and animal husbandry, for instance as a dietary supplement, feed additive, or growth promotor. The food chain is the important route for introducing enterococci into the human gut. The pathogenicity of Enterococcus from probiotic products requires investigation. In the study, 110 commercial probiotic products used for human, animal, aquaculture, and plants were examined, among which 36 enterococci were identified, including 31 from Enterococcus faecium, 2 from E. faecalis, 2 from E. casseliflavus, and 1 from E. gallinarum. Strikingly, 28 of the 36 enterococci isolated from probiotics here did not mention the presence of Enterococcus in the labeled ingredients, and no Enterococcus isolates were found from 5 animal probiotics that were labeled with the genus. In total, 35 of the 110 products exhibited hemolysis, including 5 (10.6%) human probiotics, 14 (41.2%) animal probiotics, 8 (57.1%) aquaculture probiotics, and 8 (53.3%) plant probiotics. The detection rates of virulence factors associated with adhesion, antiphagocytosis, exoenzyme, biofilm, and other putative virulence markers (PVM) in 36 enterococci were 94.4%, 91.7%, 5.6%, 94.4% and 8.3%. Twenty-six of the 36 isolated strains exhibited biofilm formation ability, where 25 strains (69.4%) and one (2.8%) were strong and weak biofilm producers, respectively. We analyzed the resistance rates against erythromycin (97%), vancomycin and ciprofloxacin (8%), tetracycline (3%), and high-level aminoglycosides (0%), respectively. High detection rates of msrC/lsaA (86%) and aac(6′)-Ii (86%) were observed, followed by vanC (8%), tetM (3%). The Tn5801-tetM-like integrative conjugative element (ICE) was identified in E. gallinarum, exhibiting resistance to tetracycline (64 μg/mL). Seven probiotic E. faecalis and E. faecium, as active ingredients in human probiotics, shared the same STs (sequence types) and were distinct from the STs of other contaminated or mislabeled enterococci, indicating that two particular STs belonged to native probiotic isolates. These findings advocate appropriate assessments of enterococci when used in probiotics.
Editorial for Special Issue “Mineralogy of Meteorites”
Authors: Sharygin Victor V.
Published: March 2021

Over 435 mineral species have been found in different types of meteorites (updated list for 2016) [...]
Electrografting of 4-Nitrobenzenediazonium Salts on Al-7075 Alloy Surfaces—The Role of Intermetallic Particles
Authors: Su Jiangling , Calderón Gómez Juan Carlos , Grundmeier Guido , González Orive Alejandro
Published: March 2021

In this work, the electrografting of Al-7075 aluminium alloy substrates with 4-nitrobenzenediazonium salt (4-NBD) films was studied on a complex aluminium alloy surface. Prior to the electrografting reaction, the substrates were submitted to different surface treatments to modify the native aluminium oxide layer and unveil intermetallic particles (IMPs). The formation of the 4-NBD films could be correlated with the passive film state and the distribution of IMPs. The corresponding electrografting reaction was performed by cyclic voltammetry which allowed the simultaneous analysis of the redox reaction by a number of complementary surface-analytical techniques. Spatially resolved thin film analysis was performed by means of SEM-EDX, AFM, PM-IRRAS, Raman spectroscopy, XPS, and SKPFM. The collected data show that the 4-NBD film is preferentially formed either on the Al oxide layer or the IMP surface depending on the applied potential range. Potentials between −0.1 and −1.0 VAg/AgCl mostly generated nitrophenylene films on the oxide covered aluminium, while grafting between −0.1 and −0.4 VAg/AgCl favours the growth of these films on IMPs.
Adherence to a Supplemented Mediterranean Diet Drives Changes in the Gut Microbiota of HIV-1-Infected Individuals
Authors: Pastor-Ibáñez Roque , Blanco-Heredia Juan , Etcheverry Florencia , Sánchez-Palomino Sonsoles , Díez-Fuertes Francisco , Casas Rosa , Navarrete-Muñoz María Ángeles , Castro-Barquero Sara , Lucero Constanza , Fernández Irene , Leal Lorna , Benito José Miguel , Noguera-Julian Marc , Paredes Roger , Rallón Norma , Estruch Ramón , Torrents David , García Felipe
Published: March 2021

Objective: The health effects of a supplemented Mediterranean diet (SMD) with extra-virgin olive oil (EVOO) and nuts are well documented in non-HIV-infected individuals. We hypothesised that the benefits of an SMD could be mediated by changes in the gut microbiota, even in those with an altered intestinal microbiota such as people living with HIV. Design: Individuals living with HIV (n = 102) were randomised to receive an SMD with 50 g/day of EVOO and 30 g/day of walnuts (SMD group) or continue with their regular diet (control group) for 12 weeks. Methods: Adherence to the Mediterranean diet was assessed using the validated 14-item MD-Adherence-Screener (MEDAS) from the PREDIMED study. A sub-study classifying the participants according to their MEDAS scores was performed. Results: The lipid profile was improved in the SMD group vs. that in the control group (delta-total cholesterol and delta-B-lipoprotein). The immune activation (CD4+HLADR+CD38+ and CD8+HLADR+CD38+ cells) and IFN-γ-producing T-cells significantly decreased at week 12 compared to the baseline in the SMD group but not in the control group. The gut microbiota in those from the high-adherence group presented significantly high diversity and richness at the end of the intervention. Succinivibrio and Bifidobacterium abundances were influenced by the adherence to the MD and significantly correlated with Treg cells. Conclusion: The Mediterranean diet improved metabolic parameters, immune activation, Treg function, and the gut microbiota composition in HIV-1-infected individuals. Further, Mediterranean diet increased the Bifidobacterium abundances after the intervention, and it was associated to a beneficial profile.
Inhibitory Effects of Breast Milk-Derived Lactobacillus rhamnosus Probio-M9 on Colitis-Associated Carcinogenesis by Restoration of the Gut Microbiota in a Mouse Model
Authors: Xu Haiyan , Hiraishi Keizo , Kurahara Lin-Hai , Nakano-Narusawa Yuko , Li Xiaodong , Hu Yaopeng , Matsuda Yoko , Zhang Heping , Hirano Katsuya
Published: March 2021

Chronic inflammation is a risk factor for colorectal cancer, and inflammatory cytokines secreted from inflammatory cells and active oxygen facilitate tumorigenesis. Intestinal bacteria are thought to regulate tumorigenesis. The longer the breastfeeding period, the lower is the risk of inflammatory bowel disease. Here, we investigated preventive effects of the probiotic Lactobacillus rhamnosus M9 (Probio-M9) on colitis-associated tumorigenesis. An inflammatory colorectal tumor model was established using a 6-week-old male C57BL/6NCrSlc mouse, which was intraperitoneally administered with azoxymethane (AOM: 12 mg/kg body weight). On weeks 2 and 4, 2% dextran sulfate sodium (DSS) was administered to mice for 7 days through drinking water. On weeks 8 and 10, Probio-M9 (2 × 109/day) was orally administered for 7 days. Animals were sacrificed at 20 weeks after AOM administration and immunohistochemical staining and Western blotting was performed. The α-diversity of microflora (Shannon index), principal coordinate analysis, and distribution of intestinal bacterium genera and metabolic pathways were compared. The AOM/DSS group showed weight loss, diarrhea, intestinal shortening, increased number of colon tumors, proliferating tumorigenesis, increased inflammation score, fibrosis, increased CD68+, or CD163+ macrophage cells in the subserosal layer of non-tumor areas. Inflammation and tumorigenesis ameliorated after Probio-M9 treatment. Fecal microbial functions were altered by AOM/DSS treatment. Probio-M9 significantly upregulated the fecal microbial diversity and reversed fecal microbial functions. Thus, Probio-M9 could suppress tumor formation in the large intestine by regulating the intestinal environment and ameliorating inflammation, suggesting its therapeutic potential for treatment of inflammation and colitis-associated tumorigenesis.
Shikonin Derivatives from Onsoma visianii Decrease Expression of Phosphorylated STAT3 in Leukemia Cells and Exert Antitumor Activity
Authors: Todorovic Zeljko , Milovanovic Jelena , Arsenijevic Dragana , Vukovic Nenad , Vukic Milena , Arsenijevic Aleksandar , Djurdjevic Predrag , Milovanovic Marija , Arsenijevic Nebojsa
Published: March 2021

Antitumor effects of shikonins on chronic lymphocytic leukemia (CLL) and B-cell prolymphocytic leukemia (B-PLL) are mostly unexplored. The antitumor activity of shikonins, isolated from Onosma visianii Clem (Boraginaceae), in BCL1, mouse CLL cells and JVM-13, human B-PLL cells was explored in this study. The cytotoxicity of shikonin derivatives was measured by an MTT test. Cell death, proliferation, cell cycle, and expression of molecules that control these processes were analyzed by flow cytometry. Expression of STAT3-regulated genes was analyzed by real-time q-RT-PCR (Quantitative Real-Time Polymerase Chain Reaction). The antitumor effects of shikonin derivatives in vivo were analyzed, using flow cytometry, by detection of leukemia cells in the peripheral blood and spleens of mice intravenously injected with BCL1 cells. The two most potent derivatives, isobutyrylshikonin (IBS) and α-methylbutyrylshikonin (MBS), induced cell cycle disturbances and apoptosis, inhibited proliferation, and decreased expression of phospho-STAT3 and downstream-regulated molecules in BCL1 and JVM-13 cells. IBS and MBS decreased the percentage of leukemia cells in vivo. The link between the decrease in phosphorylated STAT3 by MBS and IBS and BCL1 cell death was confirmed by detection of enhanced cell death after addition of AG490, an inhibitor of Jak2 kinase. It seems that IBS and MBS, by decreasing STAT3 phosphorylation, trigger apoptosis, inhibit cell proliferation, and attenuate leukemia cell stemness.
Association of Dietary Total Antioxidant Capacity with Bone Mass and Osteoporosis Risk in Korean Women: Analysis of the Korea National Health and Nutrition Examination Survey 2008–2011
Authors: Kim Donghyun , Han Anna , Park Yongsoon
Published: March 2021

Antioxidant intake has been suggested to be associated with a reduced osteoporosis risk, but the effect of dietary total antioxidant capacity (TAC) on bone health and the risk of osteoporosis remains unclear. We aimed to assess the hypothesis that dietary TAC is positively associated with bone mass and negatively related to the risk of osteoporosis in Korean women. This cross-sectional study was performed using data from the Korea National Health and Nutrition Examination Survey. Dietary TAC was estimated using task automation and an algorithm with 24-h recall data. In total, 8230 pre- and postmenopausal women were divided into four groups according to quartiles of dietary TAC. Dietary TAC was negatively associated with the risk of osteoporosis (odds ratio, 0.73; 95% confidence interval, 0.54–0.99; p-value = 0.045) in postmenopausal women, but not in premenopausal women. Dietary TAC was positively associated with bone mineral content (BMC) and bone mineral density of the femoral neck and lumbar spine in postmenopausal women and BMC of the total femur and lumbar spine in premenopausal women. Our study suggests that dietary TAC is inversely associated with the risk of osteoporosis in postmenopausal women and positively associated with bone mass in both pre- and postmenopausal women.