bluuebunny/crossref_metadata_2025 huggingface dataset:
Geologic Characteristics and Age of Beryllium Mineralization in the Jiulong Area, the Southeast Edge of the Western Kunlun–Songpan–Ganzi Rare Metal Metallogenic Belt
Authors: Hu Junliang , Zhou Jiayun , Tan Hongqi , Ni Zhiyao , Zhu Zhimin , Niu Teng , Liu Yingdong
Published: February 2025

Rare metals such as lithium and beryllium are strategic mineral resources that play a highly significant role in the national aerospace, defense, and new energy industries. The western Kunlun–Songpan–Ganzi metallogenic belt is an important rare metal metallogenic belt in China that mainly consists of granite–pegmatite-type lithium–beryllium deposits with uncommon beryllium-only deposits. In the Jiulong area on the southeastern edge of this metallogenic belt, several deposits, including the Daqianggou lithium–beryllium, Luomo beryllium, Baitai beryllium, and Shangjigong beryllium deposits, have been identified. Unlike the northern areas of Jiajika, Ke’eryin, Zawulong, and the western regions of Dahongliutan and Bailongshan, this area contains beryllium-only deposits. In this paper, we examine representative beryllium deposits in the Jiulong area, including detailed petrographic observations and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) U-Pb isotope dating of cassiterite and columbite–tantalite, to define the metallogenic age and summarize the spatiotemporal characteristics of the beryllium mineralization in this area. The research results show that the Daqianggou lithium–beryllium deposit is dominated by spodumene and beryl mineralization, while the Luomo and Baitai beryllium deposits primarily feature beryl mineralization. The dating results indicate that the U-Pb ages of the cassiterite and columbite–tantalite in the Daqianggou lithium–beryllium deposit are 157.3 ± 1.7 Ma and 164.1 ± 0.8 Ma, respectively. For the Luomo beryllium deposit, the U-Pb ages of the cassiterite and columbite–tantalite are 156.1 ± 1.5 Ma and 163.3 ± 0.8 Ma, respectively. For the Baitai beryllium deposit, the U-Pb age of the columbite–tantalite is 188.8 ± 1.1 Ma. Therefore, the Jiulong area experienced two pegmatite-type rare metal metallogenic events: a beryllium–niobium–tantalum–molybdenum event at 197~189 Ma and a lithium–beryllium–niobium–tantalum–rubidium event at 164~156 Ma. Based on the reported metallogenic ages, we suggest that the western Kunlun–Songpan–Ganzi rare metal metallogenic belt experienced three rare metal metallogenic events at 210~200 Ma, 200~180 Ma, and 170~150 Ma. Regarding exploration directions, early Yanshanian beryllium mineralization predominates in the Jiulong area along the southeastern edge of the belt, and deep exploration of the early Yanshanian rare metal mineralization within this belt should be strengthened to facilitate new breakthroughs.
The Compactified D-Brane Cylinder Amplitude and T Duality
Authors: Lu Jian-Xin
Published: February 2025

In this paper, we address how to implement T-duality to the closed string tree cylinder amplitude between a Dp brane and a Dp′ brane with p−p′=2n. To achieve this, we compute the closed string tree cylinder amplitude, for the first time, between these two D-branes with common longitudinal and transverse circle compactifications. We then show explicitly how to perform a T-duality for this amplitude along either a longitudinal or a transverse compactified direction to both branes. At the decompactification limit, we show that either the compactified cylinder amplitude or the T dual compactified cylinder gives the known non-compactified one as expected.
Antiprotozoal Aminosteroids from Pachysandra terminalis
Authors: Schäfer Lizanne , Cal Monica , Kaiser Marcel , Mäser Pascal , Schmidt Thomas J.
Published: February 2025

Trypanosoma brucei rhodesiense (Tbr) and Plasmodium falciparum (Pf) are protozoan parasites that cause severe diseases, namely, Human African Trypanosomiasis (HAT) and Malaria. Due to limited treatment options, there is an urgent need for new antiprotozoal drugs. Pachysandra terminalis (P. terminalis), a plant belonging to the family Buxaceae, is known as a rich source of aminosteroid alkaloids, and a previous study of our working group already showed that the alkaloid-enriched fraction of P. terminalis aerial parts showed promising activity against protozoan parasites. In the present study, the alkaloid-enriched fraction obtained from a 75% ethanol extract of aerial parts was separated to isolate a chemically diverse array of Pachysandra alkaloids for assessment of their antiprotozoal activity and later structure–activity studies. This work yielded a new megastigmane alkaloid (1), 7 new aminosteroids (2, 7, 16, 17, 18, 19, 20), along with 10 known aminosteroids (3–5, 8, 10–15) and 2 artifacts (6, 9) that were formed during the isolation process. The structures were elucidated by UHPLC/+ESI-QqTOF-MS/MS, as well as extensive 1- and 2D-NMR measurements. The extract and its fractions, as well as the isolated compounds, were tested in vitro against Tbr and Pf, as well as cytotoxicity against mammalian cells (L6 cell line). The activity (IC50 values) of the isolated alkaloids ranged between 0.11 and 26 µM (Tbr) and 0.39 and 80 µM (Pf). 3α,4α-diapachysanaximine A (7) showed the highest activity against Tbr (IC50 = 0.11 µM) with a selectivity index (SI) of 133 and was also quite active against Pf with IC50 = 0.63 µM (SI = 23). This compound is, therefore, a promising new antiprotozoal target for further investigations.
RETRACTED: Abdel-Shafi et al. Isolation and Characterization of Antibacterial Conglutinins from Lupine Seeds. Molecules 2023, 28, 35
Authors: Abdel-Shafi Seham , El-Nemr Mona , Enan Gamal , Osman Ali , Sitohy Basel , Sitohy Mahmoud
Published: February 2025

Following publication, concerns were brought to the attention of the Editorial Office regarding image inconsistencies within this study [...]
Determination of the Enantiomerization Barrier of Midazolam in Aqueous Conditions by Electronic Circular Dichroism and Dynamic Enantioselective HPLC/UHPLC
Authors: Mammone Francesca Romana , Sadutto Daniele , Antoniella Eleonora , Pierini Marco , Cirilli Roberto
Published: February 2025

Midazolam is a benzodiazepine that is utilized for the induction of anesthesia and the facilitation of procedural sedation. Despite the absence of stereogenic centers, the non-planar seven-membered ring devoid of reflection symmetry elements confers planar stereogenicity to the molecule. Due to the rapid conformational inversion of the Rp and Sp enantiomers, which occurs via a simple ring flip, high-performance liquid chromatography (HPLC) enantiomeric separation is restricted to sub-room temperature conditions. In this study, the energy barriers for the racemization of midazolam at five distinct temperatures and in acetonitrile/water mixtures were determined by monitoring the decay of the circular dichroism signal at a specific wavelength over time. The kinetic and thermodynamic data obtained were compared with those determined by dynamic enantioselective high-performance liquid chromatography using the Chiralpak IG-3 chiral stationary phase, which contains the amylose tris(3-chloro-5-methylphenylcarbamate) as the selector. The temperature-dependent dynamic HPLC of midazolam was carried out at the same temperatures and with the same aqueous mixtures used in parallel kinetic off-column experiments. To simulate dynamic chromatographic profiles, a lab-made computer program based on a stochastic model was utilized. The results indicated that the moderate influence of the stationary phase resulted in a slight increase in the activation barriers, which was more pronounced as the time spent in the column increased. This phenomenon was found to be mitigated when switching from a 250 mm × 4.6 mm, 3 µm, Chiralpak IG-3 column to a 50 mm × 4.6 mm, 1.6 µm, Chiralpak IG-U UHPLC column. The outcomes obtained under UHPLC conditions were found to be more closely aligned with those obtained through the ECD technique, with a discrepancy of only 0.1 kcal/mol or less, indicating a high degree of concordance between the two methods.
Chiral Recognition Mechanism of Benzyltetrahydroisoquinoline Alkaloids: Cyclodextrin-Mediated Capillary Electrophoresis, Chiral HPLC, and NMR Spectroscopy Study
Authors: Várnagy Erzsébet , Tóth Gergő , Hosztafi Sándor , Dobó Máté , Fejős Ida , Béni Szabolcs
Published: February 2025

The tetrahydroisoquinoline skeleton is a pharmacologically significant core structure containing chiral centers, making enantiomeric separation crucial due to the potentially distinct biological effects of each enantiomer. In this study, laudanosine (N-methyl-tetrahydropapaverine) and its three derivatives (6′-bromo-laudanosine, norlaudanosine, and N-propyl-norlaudanosine) were synthesized and used as model compounds to investigate chiral recognition mechanisms. Screening over twenty cyclodextrins (CyDs) as chiral selectors in capillary electrophoresis (CE), we found anionic CyDs to be the most effective, with sulfated-γ-CyD (S-γ-CyD) achieving a maximum Rs of 10.5 for laudanosine. Notably, octakis-(6-deoxy-6-(2-carboxyethyl)-thio)-γ-CyD (sugammadex, SGX), heptakis-(2,3-O-diacetyl-6-O-sulfo)-β-CD (HDAS), heptakis-(2,3-O-dimethyl-6-O-sulfo)-β-CD (HDMS), and octakis-(2,3-O-dimethyl-6-O-sulfo)-γ-CD (ODMS) provided excellent enantioseparation for all four analytes. Following HPLC screening on CyD-based and polysaccharide-based chiral stationary phases, semi-preparative HPLC methods using amylose and cellulose-based columns were optimized to isolate enantiomers. The purity of the isolated enantiomers was evaluated by HPLC, and their configurations were confirmed via circular dichroism spectroscopy. The isolated enantiomers allowed us to explore enantiomer migration order reversals in CE and enantiomer elution order reversal in HPLC. Further 1H and 2D ROESY NMR experiments provided atomic-level insights into enantioselective complex formation, confirming enantiomer differentiation by SGX and elucidating the inclusion complex structure, where the ring C immersion into the CyD cavity is prevalent.
Perceptions of Parents and Children About Videogame Use
Authors: Franzò Michela , Olivieri Gaia Maria , Salerni Anna , Iosa Marco
Published: February 2025

This study aims to investigate the gap in perceptions of parents and children on the use of videogames in childhood. Methods: A survey was conducted with 75 pairs formed by a son or daughter and one parent. The data collected contradict the prejudice that playing video games reduces study time and leads to lower grades at school (R < 0.13). Our results support the idea that playing together fosters bonding and facilitates conversation. The impact of videogames on mood showed the most substantial differences in perception, with parents mainly reporting negative mood changes, while children reported similar frequencies of negative, neutral, and positive ones. In relation to the educational and informative potential of videogames, children had slightly more positive opinions than their parents (p < 0.001). Finally, more than half of the participants potentially agreed with the possibility of using videogames as academic tools. In conclusion, there is a gap between parents’ and children’s perceptions about videogaming, especially concerning their effects on children’s mood. Playing together and developing deeper knowledge about videogames could enhance positive effects on children’s development as well as their relationships with peers, parents, and at school.
Fluorescence Quenching of Graphene Quantum Dots from Orange Peel for Methyl Orange Detection
Authors: Li Weitao , Liu Yang , Pang Xinglong , Huang Yuanhao , Dong Zeyun , Niu Qian , Xiong Yuping , Li Shang , Li Shuai , Wang Lei , Guo Huazhang , Cui Saisai , Hu Shenpeng , Li Yuenan , Cha Tiantian , Wang Liang
Published: February 2025

Methyl orange (MO) is an organic synthetic dye widely used in laboratory and industrial applications. In laboratory settings, it serves as an acid–base indicator due to its distinct color change in both acidic and alkaline environments. Industrially, it is primarily utilized in the textile industry for its ultraviolet (UV) absorption properties. However, the discharge and leakage of methyl orange into the environment can cause severe ecological damage and pose potential carcinogenic and teratogenic risks to human health. Therefore, detecting and quantifying the concentration of methyl orange in various matrices is crucial. This study reports the synthesis of graphene quantum dots (GQDs) from orange peel as a precursor, using ethanol and dimethylformamide (DMF) as solvents. Cyan (c-GQDs) and yellow (y-GQDs) graphene quantum dots were synthesized through a bottom-up hydrothermal method. The difference in color is attributed to the redshift caused by the varying ratio of pyridine nitrogen to pyrrole nitrogen. These GQDs exhibited notable optical properties, with c-GQDs emitting cyan fluorescence and y-GQDs emitting yellow fluorescence under UV light. To investigate fluorescence quenching effects, nine commonly used dyes were tested, and all were found to quench the fluorescence of y-GQDs, with methyl orange having the most significant effect. The fluorescence quenching of orange peel-derived GQDs in the presence of methyl orange is attributed to poor dispersion in DMF solution. Additionally, the GQDs possess high specific surface area, abundant surface functional groups, and excellent electronic conductivity, which contribute to their effective fluorescence quenching performance. The average thickness of y-GQDs (the vertical dimension from the substrate upwards) was 3.51 nm, confirming their graphene-like structure. They emitted yellow fluorescence within the wavelength range of 450–530 nm. Notably, a significant linear correlation was found between the concentration of methyl orange and the fluorescence intensity of y-GQDs (regression coefficient = 0.9954), indicating the potential of GQDs as effective sensing materials for organic pollutant detection.
Polymethoxyflavones and Bone Metabolism
Authors: Hirata Michiko , Tominari Tsukasa , Matsumoto Chiho , Kasuga Urara , Ikeda Keisuke , Miyaura Chisato , Grundler Florian M. W. , Inada Masaki
Published: February 2025

Phytochemicals, such as flavonoids, are bioactive compounds produced by plants, including citrus fruits, that exhibit antioxidant effects on mammalian cells and tissues. Polymethoxyflavones (PMFs) are a family of flavonoids found in the pulp and peel of citrus fruits, and have been reported to have potent antioxidant activity implicated in the prevention of human diseases. Several studies have shown that PMFs have a protective effect on bone resorption in mouse models of diseases, including osteoporosis, rheumatoid arthritis, and periodontal disease. PMFs significantly suppressed the differentiation of osteoclasts (bone resorptive cells) through indirect and direct mechanisms. The indirect effect of PMFs is the suppression of inflammatory mediator production, such as prostaglandin E2 (PGE2), and the reduction of osteoclastic inducers, such as the receptor activator of NF-κB ligand (RANKL), in osteoblasts (bone-forming cells). The direct effect of PMF suppresses osteoclast differentiation and function by inhibiting the NF-κB signaling pathway. In silico molecular docking studies indicated that PMFs target the ATP-binding pocket of IKKβ and inhibit the NF-κB signaling pathway. These findings suggest that PMFs protect against bone destruction by interfering with the NF-κB pathway in osteoblasts and osteoclasts. In this review, we summarize the latest findings regarding the effects of PMFs on various bone resorption-related diseases in mouse models.
Acute Cardiovascular Effects of Turkish Coffee Assessed by VO2 Test: A Randomized Crossover Trial
Authors: Elsahoryi Nour A. , Ibrahim Mohammed O. , Alhaj Omar A. , Hammouh Fadwa
Published: February 2025

Background: Turkish coffee (TC), a traditional unfiltered coffee preparation method, contains unique bioactive compounds due to its specific brewing process. While TC’s cultural significance is well-documented, its acute physiological and psychological effects remain understudied. Objectives: This randomized, controlled crossover trial investigated the acute effects of a standardized TC dose (3 mg caffeine/kg body weight) on metabolic and psychological parameters in healthy female university students (n = 52, age: 20.25 ± 1.20 years). Methods: TC was prepared with a 1:1 ratio of medium and dark roasted Arabica beans. The chemical analysis showed the caffeine content to be 2.8 ± 0.3 mg/mL and the chlorogenic acid content to be 1.9 ± 0.2 mg/ml. Participants were randomized to receive either TC or water control, with a washout period of 8 weeks between treatments. Cardiovascular parameters, metabolic markers, and validated visual analog scales (VAS) were assessed at baseline, 60-, 90-, and 120-min post-consumption. Results: Heart rate showed significant time-dependent reductions in both groups (control: p < 0.05; TC: p-value < 0.01 at 60, 90, and 120 min vs. baseline). Heart rate dropped significantly in the Turkish coffee group, from 78.0 ± 10.2 bpm at baseline to 71.5 ± 9.5 bpm after 90 min (p-value = 0.002). Sleep scores also declined, from 4.38 ± 2.91 at baseline to 1.88 ± 1.45 after 120 min (p < 0.05), indicating a stimulating effect of caffeine. TC consumption significantly affected appetite sensations (p < 0.05) and sleep scores (F = 3.174, p-value = 0.029), with the TC group showing progressive reductions in sleep scores from baseline (4.38 ± 2.91) to 60 min (2.58 ± 2.04), and further decreases at 90 and 120 min. Conclusions: These findings suggest that TC exerts significant acute effects on cardiovascular function and psychophysiological parameters in healthy young females, potentially due to its unique phytochemical profile and preparation method.
Unraveling the Osteogenic Activity and Molecular Mechanism of an Antioxidant Collagen Peptide in MC3T3-E1 Cells
Authors: Wang Yali , Wang Yue , Zhuang Xiaoyan , Zhang Yonghui , Fang Baishan , Fu Yousi
Published: February 2025

Background: Osteoporosis has become an inevitable health issue with global aging, and the current drug treatments often have adverse side effects, highlighting the need for safer and more effective therapies. Collagen-derived peptides are promising alternatives due to their favorable safety profile and biological activity. This study aimed to investigate the osteogenic and anti-apoptotic properties of collagen peptide UU1 (GASGPMGPR) in addition to its antioxidant activity. Methods: The effects of UU1 were evaluated in MC3T3-E1 cells by assessing osteogenic markers, including alkaline phosphatase (ALP), Cyclin D1, runt-related transcription factor 2 (Runx2), and Akt/β-catenin signaling. Western blot analysis quantified collagen I, osteocalcin, and phosphorylated Akt levels. Anti-apoptotic effects were measured via p-Akt levels and the Bax/Bcl-2 ratio. Computational molecular docking was performed to explore the molecular mechanism of UU1 via its interaction with epidermal growth factor receptor (EGFR) and collagen-binding integrin. Results: UU1 treatment promoted cell differentiation, with elevated ALP, Cyclin D1, Runx2, and Akt/β-catenin signaling. Notably, at 0.025 mg/mL, UU1 upregulated the levels of collagen I, osteocalcin, and phosphorylated Akt by 2.14, 3.37, and 1.95 times, respectively, compared to the control. Additionally, UU1 exhibited anti-apoptotic effects, indicated by increased p-Akt levels and a reduced Bax/Bcl-2 ratio. Molecular docking analysis suggested that UU1 could assist the dimerization of EGFR, facilitating downstream signaling transductions and activating collagen-binding integrin. Conclusions: These findings highlight UU1 as a multifunctional peptide with antioxidant, osteogenic, and anti-apoptotic properties, positioning it as a promising candidate for anti-osteoporosis applications in the food and pharmaceutical industries.
Human Milk Oligosaccharides in Breast Milk at Two Weeks of Age in Relation to Neurodevelopment in 2-Year-Old Children Born Extremely Preterm: An Explorative Trial
Authors: Wejryd Erik , Freiholtz Jern Erik , Marchini Giovanna , Åden Ulrika , Landberg Eva , Abrahamsson Thomas
Published: February 2025

Background: Preventing neurodevelopmental impairment after extremely preterm birth remains challenging. While breast milk feeding is linked to better neurodevelopment, the underlying mechanisms are unclear. This study explored the association between individual human milk oligosaccharides (HMO) and neurodevelopment at two years of age in extremely preterm children. Methods: Milk samples from mothers of 76 extremely preterm infants collected at two weeks after birth were analyzed for 15 dominant HMOs. Register data from examination and Bayley-III neurodevelopmental assessment at two years’ corrected age was retrieved and categorized into levels of impairment. An exploratory analysis examined associations between the HMO composition and neurodevelopment. Results: Bioinformatic volcano plots revealed associations between specific HMOs and outcomes: 3FL with less neurodevelopmental impairment, LSTb with higher Bayley-III cognitive scores, and LSTa with worse neurodevelopmental impairment outcomes. Spearman correlations indicated LSTa was linked to more neurodevelopmental impairment (p = 0.018), lower language (p = 0.009), and motor (p = 0.02) scores, whereas 3FL correlated with less neurodevelopmental impairment (p = 0.02). Dichotomized analysis showed LSTa was associated with more neurodevelopmental impairment and lower language scores (p < 0.05), 3FL with milder neurodevelopmental impairment (p < 0.05), and LSTb with better cognitive (p < 0.01) and language (p < 0.05) scores. No significant associations were found for HMO diversity, total sialic acid content, or secretor/Lewis patterns. Conclusions: In this explorative hypothesis-generating study, certain HMOs appeared to be associated with both potentially beneficial and adverse neurodevelopmental outcomes in extremely preterm infants. However, these findings should be interpreted with caution, as they do not constitute evidence but rather serve as a preliminary foundation for future hypothesis-driven research.
Interplay Between Diet, Branched-Chain Amino Acids, and Myokines in Children: Vegetarian Versus Traditional Eating Habits
Authors: Ambroszkiewicz Jadwiga , Chełchowska Magdalena , Mazur Joanna , Rowicka Grażyna , Klemarczyk Witold , Strucińska Małgorzata , Gajewska Joanna
Published: February 2025

Background/Objectives: The quality and composition of dietary proteins are crucial during growth, particularly in children who follow vegetarian diets. Branched-chain amino acids (BCAAs: leucine, isoleucine, and valine) and lysine play essential roles in muscle growth, repair, and metabolism and are involved in the regulation of muscle-derived proteins known as myokines. This study aimed to compare the dietary intake and circulating levels of BCAAs, lysine, and myokines—follistatin-like protein 1 (FSTL-1), myostatin, and myonectin—between vegetarian and omnivorous prepubertal children and to explore the impact of diet on muscle metabolism. Methods: Sixty-four healthy Caucasian children aged 4–9 years (forty-two vegetarians and twenty-two omnivores) were assessed for dietary intake using the Dieta 5® (extended version Dieta 5.0) software. Circulating BCAAs and lysine were measured using high-performance liquid chromatography, while myokine concentrations were determined using enzyme-linked immunosorbent assays. Results: Vegetarian children showed significantly lower intakes of total protein, animal protein, BCAAs, and lysine than omnivores. Correspondingly, the circulating levels of isoleucine, valine, lysine, and albumin were significantly reduced in vegetarians. Among myokines, serum myostatin and myonectin levels were comparable between the groups, but vegetarians had significantly lower median FSTL-1 levels 7.7 (6.5–9.4) ng/mL than omnivores 9.7 (7.5–13.9) ng/mL (p = 0.012). In the entire group of children, positive correlations were observed between dietary total and animal protein intake and circulating valine and lysine levels. Dietary animal protein intake was also positively associated with the serum levels of all myokines, whereas plant protein intake was negatively correlated with myonectin concentration. Conclusions: In conclusion, vegetarian diets in prepubertal children are associated with reduced dietary protein quality and lower circulating BCAAs, lysine, and FSTL-1 levels, which may impact muscle metabolism. Optimizing vegetarian diets using high-quality plant proteins with proper essential amino acids could mitigate their deficiencies and support muscle development during critical growth periods.
Clinical Evaluation of Ganoderma lucidum Spore Oil for Triglyceride Reduction: A Randomized, Double-Blind, Crossover Study
Authors: Wang Xinyi , Wang Xufeng , Zhao Liang , Zhou Feng
Published: February 2025

Background: Ganoderma lucidum spore oil (GLSO) is widely recognized for its notable medicinal and nutritional properties. This study aimed to systematically evaluate the efficacy and safety of GLSO extract in individuals with dyslipidemia. Methods: In a randomized, double-blind, placebo-controlled trial, 110 participants were enrolled and randomly assigned to either the intervention group or the placebo group. A chi-square test of baseline characteristics confirmed no significant differences in age or sex distribution between the two groups. Results: After 12 weeks of intervention, the intervention group exhibited significantly lower levels of total cholesterol (CHO), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C), alongside significantly higher levels of high-density lipoprotein cholesterol (HDL-C), compared to the placebo group, with all differences reaching statistical significance. Furthermore, the relative percentage changes in lipid parameters also demonstrated significant intergroup differences. Safety analyses revealed that the intervention had no notable effects on renal function parameters, whereas hepatic function parameters showed statistically significant improvement in the intervention group. Conclusions: This study demonstrated that GLSO extract effectively improved lipid profiles and liver function, with a favorable safety and tolerability profile. These findings strongly support the potential clinical application of GLSO extract in the management of dyslipidemia.
Enhanced Antioxidant and Protective Effects of Fermented Solanum melongena L. Peel Extracts Against Ultraviolet B-Induced Skin Damage
Authors: Lee Joo Hwa , Kim Jinsick , Jo Yu Chang , Jo Yun Hoo , Jeong Yeong Hwan , Jeong Soo Ah , Lim Beong Ou , Shin Dong Wook
Published: February 2025

Background/Objectives: The skin, being the body’s outermost organ, plays a vital role in protecting against various external stimuli. Ultraviolet generates reactive oxygen species (ROS), promoting the secretion of matrix metalloproteinases (MMPs) and inducing collagen degradation. Many studies have been conducted to identify natural substances that can prevent or delay the harmful effects of UV. Methods: A wound healing assay, DCF-DA reactive oxygen species (ROS) assay, and JC-1 assay were performed to assess the effects of bio-converted eggplant peels (BEPs) on human dermal fibroblasts (HDFs). Western blot analysis was also conducted to understand the underlying mechanisms for their effects. Finally, hematoxylin–eosin staining and immunohistochemistry were also performed in animal studies. Results: Our study evaluated the antioxidant efficacy of BEPs fermented with Lactobacillus plantarum in hydrogen peroxide (H2O2)-HDFs and UVB-induced skin damage in hairless mice. We demonstrated that BEPs exhibited enhanced antioxidant properties compared to non-fermented eggplant peels (EPs). BEPs facilitated wound healing in H2O2-damaged HDFs, reduced ROS levels, and restored mitochondrial membrane potential. BEPs suppressed the phosphorylation of ERK, p38, and JNK as their underlying mechanism. We further demonstrated that dietary supplementation of BEPs also downregulated matrix metalloproteinase 1 (MMP1) expression and upregulated collagen I (COL1) in UVB-damaged hairless mice, indicating that BEPs were more effective compared to EPs. Conclusions: Our studies suggest that BEPs fermented with Lactobacillus plantarum hold significant potential as a protective agent for mitigating UVB-induced damage and promoting skin health.
Public Primary School Compliance with School Canteen Policy in Riyadh, Saudi Arabia: A Cross-Sectional Study
Authors: Alsiwat Areej , Kitsaras George , Glenny Anne-Marie , Alayadi Haya , Goodwin Michaela
Published: February 2025

Background/Objectives: The Ministry of Health and Education in Saudi Arabia established school canteen guidelines that have been mandatory since 2014. Only one previous study has investigated the compliance of public high schools with these guidelines. The aim of this study is to explore public primary school compliance with the school canteen policy guidelines set by the Saudi Ministry of Health and Education. Methods: A cross-sectional study with a total of 80 public primary schools randomly selected from five regions in Riyadh, Saudi Arabia, was conducted. School canteens were explored using a school canteen checklist provided by the Ministry of Education. The checklist contains a list of items grouped into healthy and unhealthy foods. A final assessment for alignment with the checklist was classified as either poor, average, or good. Canteen staff were provided with a structured questionnaire on the day of the school visit to gather their opinions and feedback regarding the canteen. Results: A total of 70% of schools showed an average rating for alignment with the Ministry of Health and Education guidelines; however, most of the items available in canteens were unhealthy. The canteen staff recommended adding healthier options like milk, fruit, and vegetables to school canteens. Furthermore, canteen staff reported issues in regard to expensive foods and a lack of choice in healthy food options. Conclusions: This study has shown that public primary schools in Riyadh, Kingdom of Saudi Arabia (KSA), have an average rating alignment with the Ministry of Health and Education’s school food policy guidelines. This study highlights the need for improvement in the Saudi Ministry of Education’s checklist, particularly regarding the availability of healthy foods, including milk, fruits, vegetables, and other nutritious items.
Association Between Aflatoxin Exposure and Haemoglobin, Zinc, and Vitamin A, C, and E Levels/Status: A Systematic Review
Authors: Mshanga Naelijwa , Moore Sally , Kassim Neema , Martin Haikael D. , Auma Carolyn I. , Gong Yun Yun
Published: February 2025

Background: Aflatoxin, produced by Aspergillus flavus and Aspergillus parasiticus fungi, contaminates a broad range of crops such as maize, nuts, and cotton. Aflatoxin exposure causes growth failure, immune suppression, and liver cancer. While several systematic reviews have assessed the link between aflatoxin exposure and growth development in humans, there is a lack of reviews on the associations between aflatoxin exposure and micronutrient levels/status. This review addresses that gap by compiling studies on the association between aflatoxin exposure and micronutrient levels/status in humans. Methods: A comprehensive search of the SCOPUS, PUBMED, EMBASE, and Web of Science databases was conducted, focusing on studies published between 2003 and 2023. Only English-language studies using urine, blood, serum, or plasma biomarkers were included to assess the exposure and outcomes. The risk of bias in these studies was evaluated using the Academy of Nutrition and Dietetics Quality Criteria for human studies. Results: Ten observational studies were included in the systematic review, which collectively reported the association between aflatoxin exposure and haemoglobin, zinc, and vitamin A, E, and C levels. This review suggests that aflatoxin exposure is associated with micronutrient deficiencies, such as anaemia (low haemoglobin levels (<11 g/dL)) in pregnant women and vitamin A deficiency in adults and children. Conclusions: This review highlights the link between aflatoxin exposure and micronutrient deficiencies, emphasizing the need for aflatoxin mitigation within micronutrient interventions. Future studies should focus on longitudinal and interventional research to establish causal relationships and assess the effectiveness of mitigation strategies. Additionally, further research is needed to explore the interaction between aflatoxin exposure and other potential confounding factors such as dietary patterns, socioeconomic status, and genetic predisposition.
Consumption of the Food Groups with the Revised Benefits in the New WIC Food Package: A Scoping Review
Authors: Zhang Qi , Patel Priyanka T. , Neupane Bidusha , Lowery Caitlin M. , Alkhalifah Futun , Mahdavi Faezeh , Sarino Esther May
Published: February 2025

Background: On 18 April 2024, the United States Department of Agriculture (USDA) published the first food package changes to the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) in over a decade, which reduced some food benefits (juice, milk, canned fish, and infant fruits and vegetables) and offered substitutes (cash-value vouchers (CVVs) or cash-value benefits (CVBs) to redeem for fruits and vegetables, cheese, soymilk, or other dairy products). Methods: To assess the impact of the changes on the consumption and redemption of these food groups, a systematic search was conducted, identifying 23 peer-reviewed publications between 2010 and 2024. Results: They revealed significant shifts in consumption after the 2009 food package changes; e.g., a decline in 100% juice intake following reductions in juice allowances. Additionally, the review highlighted that the 2009 WIC food package revision was associated with more fruit and vegetable consumption after the increase in CVV allowance. While including milk alternatives like soy-based beverages or lactose-free milk or cheese may improve redemption rates and WIC program satisfaction, the long-term impacts of these proposed changes remain unknown. No research was identified on the consumption of canned fish. Conclusions: This review contributes to understanding the changes in redemption and consumption after the last WIC food package changes, identifies the knowledge gap about prospective impacts, and recommends that the WIC agencies implement appropriate evaluations to promote health and nutrition among vulnerable populations.
Psychobiotics Ameliorate Depression and Anxiety Status in Surgical Oncology Patients: Results from the ProDeCa Study
Authors: Tzikos Georgios , Chamalidou Eleni , Christopoulou Dimitra , Apostolopoulou Aikaterini , Gkarmiri Sofia , Pertsikapa Marianthi , Menni Alexandra-Eleftheria , Theodorou Ioannis M. , Stavrou George , Doutsini Nektaria-Dimitra , Shrewsbury Anne D. , Papavramidis Theodosios , Tsetis Joulia K. , Theodorou Helen , Konsta Anastasia , Kotzampassi Katerina
Published: February 2025

Background: Psychological disorders are prevalent in patients having undergone gastrointestinal cancer surgery, and their emotional status may further deteriorate during subsequent chemotherapy. Psychobiotics are specific probiotics that have the unique characteristics of producing neuroactive substances that are thought to act on the brain–gut axis. The aim of the present study was to evaluate the benefits of a psychobiotic formula on depression and anxiety status, as well as on perceived stress, versus a placebo in patients on a chemotherapy course following gastrointestinal surgery for cancer. Patients: The enrolled patients, allocated to the psychobiotic and placebo groups, were assessed by means of these psychometric tests: Beck’s Depression Inventory and the Hamilton Depression Rating 17-item Scale for depression; the General Anxiety Disorder-7 for anxiety; and the Perceived Stress Scale-14 Item for perceived stress at three time-points: upon allocation [T1], after one month of treatment [T2], and two months thereafter [T3]. Results: In total, 266 patients were included. One month of psychobiotic treatment improved [i] depression status by 60.4% [48 depressed patients at T1, reduced to 16 at T3]; [ii] anxiety by 57.0% [72 patients at T1, 26 at T3]; and [iii] stress by 60.4% [42 at T1, 14 at T3]. The placebo-treated patients experienced a deterioration in all parameters studied, i.e., depression increased by 62.9%, anxiety by 39.7%, and stress by 142.5%. Conclusions: Based on these findings, it can be recognized that psychobiotic treatment has great potential for every patient at risk of suffering from depression, anxiety, or stress during the course of surgery/chemotherapy for gastrointestinal cancer.
The Detrimental Impact of Ultra-Processed Foods on the Human Gut Microbiome and Gut Barrier
Authors: Rondinella Debora , Raoul Pauline Celine , Valeriani Eleonora , Venturini Irene , Cintoni Marco , Severino Andrea , Galli Francesca Sofia , Mora Vincenzina , Mele Maria Cristina , Cammarota Giovanni , Gasbarrini Antonio , Rinninella Emanuele , Ianiro Gianluca
Published: February 2025

Ultra-processed foods (UPFs) have become a widely consumed food category in modern diets. However, their impact on gut health is raising increasing concerns. This review investigates how UPFs impact the gut microbiome and gut barrier, emphasizing gut dysbiosis and increased gut permeability. UPFs, characterized by a high content of synthetic additives and emulsifiers, and low fiber content, are associated with a decrease in microbial diversity, lower levels of beneficial bacteria like Akkermansia muciniphila and Faecalibacterium prausnitzii, and an increase in pro-inflammatory microorganisms. These alterations in the microbial community contribute to persistent inflammation, which is associated with various chronic disorders including metabolic syndrome, irritable bowel syndrome, type 2 diabetes, and colorectal cancer. In addition, UPFs may alter the gut–brain axis, potentially affecting cognitive function and mental health. Dietary modifications incorporating fiber, fermented foods, and probiotics can help mitigate the effects of UPFs. Furthermore, the public needs stricter regulations for banning UPFs, along with well-defined food labels. Further studies are necessary to elucidate the mechanisms connecting UPFs to gut dysbiosis and systemic illnesses, thereby informing evidence-based dietary guidelines.
Redefining Roles—Fathers Play a Crucial Role in Shaping Children’s Healthy Eating Behaviors: Cross-Sectional Observational Study
Authors: Ng Nicholas Beng Hui , Ng Jamie Qiao Xin , Shen Liang , Shorey Shefaly
Published: February 2025

Background/Objectives: Fathers have been largely understudied in feeding research, as most studies have focused on mothers. This study aims to explore the relationship between paternal feeding practices and child eating behaviors. Methods: This study employed a cross-sectional observational design. Fathers (n = 104) completed one-off self-reported questionnaires using the Child Feeding Practices Questionnaire and the Child Eating Behaviors Questionnaire from October 2023 to February 2024. Variations in feeding practices across sociodemographic factors were explored using correlational statistics. The generalized linear model was used to identify relationships between paternal feeding practices and child eating behaviors. Results: For young children, the availability of healthy food at home was positively associated with food approach behaviors, while paternal practices of pressuring and child control were linked to increased food avoidance. Among school-aged children, using food as a reward was positively associated with food approach behaviors, while using food to regulate emotions was linked to increased food avoidance. Restriction for weight control was associated with both food approach and avoidant behaviors. In adolescents, paternal monitoring of their diet and certain socioeconomic conditions were observed to be associated with their eating behaviors. Conclusions: The findings provide valuable insights into the role of paternal feeding practices in shaping children’s eating behaviors and highlight the potential for interventions targeting modifiable paternal behaviors to support healthy eating habits.
The Role of the Planetary Diet in Managing Metabolic Syndrome and Cardiovascular Disease: A Narrative Review
Authors: Muszalska Aleksandra , Wiecanowska Julia , Michałowska Joanna , Pastusiak-Zgolińska Katarzyna Magdalena , Polok Izabela , Łompieś Kinga , Bogdański Paweł
Published: February 2025

Introduction: The planetary health diet, introduced by the EAT–Lancet Commission, aims to address global health and sustainability challenges by promoting a plant-based diet with reduced consumption of animal-sourced foods. This diet not only contributes to environmental sustainability but also offers significant health benefits, including prevention and management of abdominal obesity, carbohydrate metabolism disorders, dyslipidemia, and elevated blood pressure. These metabolic disorders are components of metabolic syndrome, a cluster of risk factors for cardiovascular disease. Objectives: This narrative review aims to gather the most recent findings on the impact of the planetary diet on individual components of metabolic syndrome and on the prevention and treatment of cardiovascular disease. Methods: The available research on the topic was identified via searches in PubMed, Scopus, and Google Scholar. Results: Abdominal obesity, a major risk factor for a range of chronic diseases, can be effectively mitigated by adhering to plant-based dietary patterns, which have been shown to reduce waist circumference and improve overall cardiometabolic health. Furthermore, the planetary diet plays a crucial role in reducing the risk of type-2 diabetes and improving glycemic control, with specific dietary components such as whole grains and fiber, demonstrating positive effects on blood glucose levels. This diet is additionally associated with favorable lipid profiles, including lower levels of LDL cholesterol and total cholesterol, which are critical in the prevention of atherosclerosis and cardiovascular diseases. Conclusions: These multiple benefits highlight that the planetary diet may be an effective strategy for managing and preventing metabolic syndrome and cardiovascular disease. However, further research is needed to confirm its long-term efficacy and applicability across diverse populations.
Gnetin C in Cancer and Other Diseases: What Do We Know So Far?
Authors: Campanelli Gisella , Levenson Anait S.
Published: February 2025

Stilbenes are a class of natural polyphenols with multiple positive pharmacologic assets such as antioxidant, anti-inflammatory and anticancer effects. While monomeric stilbenes, represented mostly by resveratrol and pterostilbene, have been studied intensely in the last two decades, oligomeric compounds, which may have better prospects of becoming potent nutraceuticals, are much less studied. The goal of this review is to compile all available literature to date on the beneficial pharmacologic effects of Gnetin C, a resveratrol dimer, in cancer and other diseases. While studies have shown the beneficial effects of Gnetin C, as a single compound or a component of melinjo seed extract, through cellular models, in vivo preclinical studies are still lacking. This is except for prostate cancer, where various animal models, including xenografts and transgenic mice, have been used to evaluate Gnetin C’s more potent anti-inflammatory and anticancer effects compared to resveratrol and its monomeric analogs. Since Gnetin C’s safety has already been demonstrated in healthy volunteers, it is now logical to evaluate its efficacy for prostate cancer chemoprevention, interception and therapy in clinical trials.
Association of Paternal BMI and Diet During Pregnancy with Offspring Birth Measures: The STEPS Study
Authors: Kearns Michelle L. , Lahdenperä Mirkka , Galante Laura , Rautava Samuli , Lagström Hanna , Reynolds Clare M.
Published: February 2025

Background/Objectives: Maternal Body Mass Index (BMI), diet quality, and their associated effects on offspring birth measures are well-established. Emerging evidence, largely from animal studies, has indicated paternal factors can influence offspring birth outcomes. However, this effect is poorly understood in humans. Our aim was to examine the association between paternal BMI and diet quality score and offspring birth measures. Methods: Participants from the STEPS (Steps to the healthy development) Study in Southwest Finland were recruited during the first trimester of pregnancy or after delivery. A total of 1586 fathers and their children were included for BMI analysis, and 208 fathers and their children were included for dietary analyses. Paternal BMI was calculated using self-reported weight and height at recruitment, and dietary behaviour was assessed using the Index of Diet Quality (IDQ) at 30 weeks’ gestation. Offspring birth weight and length z-scores were calculated using the recently published references specific to the Finnish population. Generalized linear model analyses were carried out to determine associations between paternal factors and offspring z-scores. Results: The mean paternal BMI was 26 (SD ± 3.5). Over half of the fathers were classed as having an unhealthy diet, classified as poor in adhering to nutrition recommendations including higher intakes of saturated fatty acids, and inadequate intakes of protein, saccharose, fibre, vitamins, and minerals. Paternal BMI was not significantly associated with offspring birth weight (β = 0.00 p = 0.884) or birth length (β = 0.00, p = 0.774) z-scores when adjusted for maternal and other paternal and parental factors. Paternal diet quality score was not associated with offspring birth weight (β = −0.01, p = 0.515) or birth length (β = 0.07 p = 0.291) z-scores. Conclusions: This study shows paternal BMI or diet quality at 30 weeks’ gestation does not significantly impact offspring birth measures. Given the known impact of nutrition on epigenetics, examining the potential influence of paternal factors at conception on offspring growth is of major importance and should be included in future studies.