bluuebunny/crossref_metadata_2025 huggingface dataset:
Modelling of River Flow Using Particle Swarm Optimized Cascade-Forward Neural Networks: A Case Study of Kelantan River in Malaysia
Authors: Hayder Gasim , Solihin Mahmud Iwan , Mustafa Hauwa Mohammed
Published: December 2020

Water resources management in Malaysia has become a crucial issue of concern due to its role in the economic and social development of the country. Kelantan river (Sungai Kelantan) basin is one of the essential catchments as it has a history of flood events. Numerous studies have been conducted in river basin modelling for the prediction of flow and mitigation of flooding events as well as water resource management. This paper presents river flow modelling based on meteorological and weather data in the Sungai Kelantan region using a cascade-forward neural network trained with particle swarm optimization algorithm (CFNNPSO). The result is compared with those trained with the Levenberg–Marquardt (LM) and Bayesian Regularization (BR) algorithm. The outcome of this study indicates that there is a strong correlation between river flow and some meteorological and weather variables (weighted rainfall, average evaporation and temperatures). The correlation scores (R) obtained between the target variable (river flow) and the predictor variables were 0.739, −0.544, and −0.662 for weighted rainfall, evaporation, and temperature, respectively. Additionally, the developed nonlinear multivariable regression model using CFNNPSO produced acceptable prediction accuracy during model testing with the regression coefficient (R2), root mean square error (RMSE), and mean of percentage error (MPE) of 0.88, 191.1 cms and 0.09%, respectively. The reliable result and predictive performance of the model is useful for decision makers during water resource planning and river management. The constructed modelling procedure can be adopted for future applications.
A Novel Real-Time Virtual 3D Object Composition Method for 360° Video
Authors: Lee Jaehyun , Ha Sungjae , Gentet Philippe , Hwang Leehwan , Kwon Soonchul , Lee Seunghyun
Published: December 2020

As highly immersive virtual reality (VR) content, 360° video allows users to observe all viewpoints within the desired direction from the position where the video is recorded. In 360° video content, virtual objects are inserted into recorded real scenes to provide a higher sense of immersion. These techniques are called 3D composition. For a realistic 3D composition in a 360° video, it is important to obtain the internal (focal length) and external (position and rotation) parameters from a 360° camera. Traditional methods estimate the trajectory of a camera by extracting the feature point from the recorded video. However, incorrect results may occur owing to stitching errors from a 360° camera attached to several high-resolution cameras for the stitching process, and a large amount of time is spent on feature tracking owing to the high-resolution of the video. We propose a new method for pre-visualization and 3D composition that overcomes the limitations of existing methods. This system achieves real-time position tracking of the attached camera using a ZED camera and a stereo-vision sensor, and real-time stabilization using a Kalman filter. The proposed system shows high time efficiency and accurate 3D composition.
Improving Tolerance Control on Modular Construction Project with 3D Laser Scanning and BIM: A Case Study of Removable Floodwall Project
Authors: Li Huimin , Zhang Chengyi , Song Siyuan , Demirkesen Sevilay , Chang Ruidong
Published: December 2020

Quality control is essential to a successful modular construction project and should be enhanced throughout the project from design to construction and installation. The current methods for analyzing the assembly quality of a removable floodwall heavily rely on manual inspection and contact-type measurements, which are time-consuming and costly. This study presents a systematic and practical approach to improve quality control of the prefabricated modular construction projects by integrating building information modeling (BIM) with three-dimensional (3D) laser scanning technology. The study starts with a thorough literature review of current quality control methods in modular construction. Firstly, the critical quality control procedure for the modular construction structure and components should be identified. Secondly, the dimensions of the structure and components in a BIM model is considered as quality tolerance control benchmarking. Thirdly, the point cloud data is captured with 3D laser scanning, which is used to create the as-built model for the constructed structure. Fourthly, data analysis and field validation are carried out by matching the point cloud data with the as-built model and the BIM model. Finally, the study employs the data of a removable floodwall project to validate the level of technical feasibility and accuracy of the presented methods. This method improved the efficiency and accuracy of modular construction quality control. It established a preliminary foundation for using BIM and laser scanning to conduct quality control in removable floodwall installation. The results indicated that the proposed integration of BIM and 3D laser scanning has great potential to improve the quality control of a modular construction project.
Hardware in the Loop Platform for Testing Photovoltaic System Control
Authors: Samano-Ortega Víctor , Padilla-Medina Alfredo , Bravo-Sanchez Micael , Rodriguez-Segura Elías , Jimenez-Garibay Alonso , Martinez-Nolasco Juan
Published: December 2020

The hardware in the loop (HIL) technique allows you to reproduce the behavior of a dynamic system or part of it in real time. This quality makes HIL a useful tool in the controller validation process and is widely used in multiple areas including photovoltaic systems (PVSs). This study presents the development of an HIL system to emulate the behavior of a PVS that includes a photovoltaic panel (PVP) and a DC-DC boost converter connected in series. The emulator was embedded into an NI-myRIO development board that operates with an integration time of 10 µs and reproduces the behavior of the real system with a mean percent error of 2.0478%, compared to simulation results. The implemented emulator is proposed as a platform for the validation of control systems. With it, the experimental stage is carried out on two controllers connected to the PVS without having the real system and allowing to emulate different operating conditions. The first controller is based on the Hill Climbing algorithm for the maximum power point tracking (MPPT), the second is a proportional integral (PI) controller for voltage control. Both controllers generate settling times of less than 3 s; the MPPT controller generates variations in the output in steady state inherent to the algorithm used. For both cases, the comparison of the experimental results with those obtained through software simulation show that the platform fulfills its usefulness when evaluating control systems.
Characterisation of Biological Materials at THz Frequencies by Attenuated Total Reflection: Lard
Authors: Vilagosh Zoltan , Lajevardipour Alireza , Appadoo Dominique , Ng Soon Hock , Juodkazis Saulius , Wood Andrew W.
Published: December 2020

The penetration depth of an evanescent wave in Attenuated Total Reflection (ATR) is dependent on the wavelength of the radiation utilised. At THz frequencies, the penetration depth into biological tissues is in the order of 0.1 to 0.5 mm; rendered pig lard was used as a model sample in this study. A method for the direct measurement of the evanescent wave penetration depth is presented which allows for the estimation of the dispersion of the complex refractive index by using the reflection of the evanescent wave from varying sample depths. The method employs frustrated total internal reflection, and has been demonstrated by using the THz/Far-IR beamline at the Australian synchrotron, and modelled using finite difference time domain (FDTD) simulations.
A Model for Assessing the Quantitative Effects of Heterogeneous Affinity in Malaria Transmission along with Ivermectin Mass Administration
Authors: Sequeira João , Louçã Jorge , Mendes António M. , Lind Pedro G.
Published: December 2020

Using an agent-based model of malaria, we present numerical evidence that in communities of individuals having an affinity varying within a broad range of values, disease transmission may increase up to 300%. Moreover, our findings provide new insight into how to combine different strategies for the prevention of malaria transmission. In particular, we uncover a relationship between the level of heterogeneity and the level of conventional and unconventional anti-malarial drug administration (ivermectin and gametocidal agents), which, when taken together, will define a control parameter, tuning between disease persistence and elimination. Finally, we also provide evidence that the entomological inoculation rate, as well as the product between parasite and sporozoite rates are both good indicators of malaria incidence in the presence of heterogeneity in disease transmission and may configure a possible improvement in that setting, upon classical standard measures such as the basic reproductive number.
Changes in the Kinematic and Kinetic Characteristics of Lunge Footwork during the Fatiguing Process
Authors: Du Yanyan , Fan Yubo
Published: December 2020

Fatigue is a major injury risk factor. The aim of this study was to investigate the effects of fatigue on lunging during the fatiguing process. The lower extremity joint kinematics and kinetics of fifteen male collegiate badminton players were simultaneously recorded by optical motion-capture and force plate systems during lunging. In addition to statistical analyses of discrete variables, one-dimensional statistical parametric mapping (SPM (1D)) was used to analyze the waveform data. The hypotheses were that the biomechanics of lunging maneuvers would change during the fatiguing process, and the fatigue effects would differ in different periods (I–V) of the stance phase and in different joints. Results showed that the initial contact angles, peak angles, moments, power, and time needed to reach the peak angles at the hip, knee, and ankle in the sagittal plane all decreased post-fatigue. A continuous decreasing tendency was reflected in the moments and power of hip and, in particular, knee joints (mostly p < 0.001). Period IV showed a significant fatigue response. In conclusion, both discrete and waveform data illustrated the effects of fatigue, however, the results of SPM (1D) analysis showed both the key period and body segments affected by the fatigue response.
Prediction of Mechanical Performance of Acetylated MDF at Different Humid Conditions
Authors: Ahmed Sheikh Ali , Adamopoulos Stergios , Li Junqiu , Kovacikova Janka
Published: December 2020

Change of relative humidity (RH) in surrounding environment can greatly affect the physical and mechanical properties of wood-based panels. Commercially produced acetylated medium density fiberboard (MDF), Medite Tricoya®, was used in this study to predict strength and stiffness under varying humid conditions by separating samples in parallel (//) and perpendicular (⊥) to the sanding directions. Thickness swelling, static moduli of elasticity (MOEstat) and rupture (MORstat), and internal bond (IB) strength were measured at three different humid conditions, i.e., dry (35% RH), standard (65% RH) and wet (85% RH). Internal bond (IB) strength was also measured after accelerated aging test. A resonance method was used to determine dynamic modulus of elasticity (MOEdyn) at the aforementioned humid conditions. Linear regression and finite element (FE) analyses were used to predict the MDF’s static bending behavior. Results showed that dimensional stability, MOEstat, MORstat and IB strength decreased significantly with an increase in RH. No reduction of IB strength was observed after 426 h of accelerated aging test. A multiple regression model was established using MOEdyn and RH values to predict MOEstat and MORstat. In both directions (// and ⊥), highly significant relationships were observed. The predicted and the measured values of MOEstat and MORstat were satisfactorily related to each other, which indicated that the developed model can be effectively used for evaluating the strength and stiffness of Medite Tricoya® MDF samples at any humid condition. Percent errors of two different simulation techniques (standard and extended FE method) showed highly efficient way of simulating the MDF structures with low fidelity.
Multi-Element Determination of Toxic and Nutrient Elements by ICP-AES after Dispersive Solid-Phase Extraction with Modified Graphene Oxide
Authors: Manousi Natalia , Deliyanni Eleni , Zachariadis George
Published: December 2020

A novel graphene-oxide-derived material was synthesized after modification of graphene oxide with sodium hydroxide and used for the dispersive solid-phase extraction (d-SPE) of different elements (Pb, Cd, Ba, Zn, Cu and Ni) prior to their determination by inductively coupled plasma atomic emission spectrometry (ICP-AES). The prepared nanomaterial was characterized by X-ray diffraction (XRD), nitrogen adsorption, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) spectroscopy. Full factorial design and Derringer’s type desirability function were used for the optimization of the d-SPE procedure. Pareto charts illustrated the effects of each of the examined factors and their interactions on the determination of the elements. Under the optimum conditions, detection limits (LODs) for the elements ranged between 0.01 and 0.21 μg g−1, intra-day repeatability (n = 5) was lower than 1.9% and inter-day repeatability (n = 5 × 3) was lower than 4.7%. Relative recovery values ranged between 88.1 and 117.8%. The method was validated and successfully applied for the determination of trace elements in poultry, pork and beef samples from the local market. The proposed method is simple, rapid, sensitive and the novel sorbent can be used at least ten times.
NIRS Estimation of Drought Stress on Chemical Quality Constituents of Taro (Colocasia esculenta L.) and Sweet Potato (Ipomoea batatas L.) Flours
Authors: Gouveia Carla S. S. , Lebot Vincent , Pinheiro de Carvalho Miguel
Published: December 2020

Taro (Colocasia esculenta (L.) Schott) and sweet potato (Ipomoea batatas (L.) Lam.) are important food crops worldwide, whose productivity is threatened by climatic constraints, namely drought. Data calibration, validation, and model development of high-precision near-infrared spectroscopy (NIRS) involving multivariate analyses are needed for the fast prediction of the quality of tubers and shoots impacted by drought stress. The main objective of this study was to generate accurate NIRS models for quality assessment of taro and sweet potato accessions (acc.) subjected to water scarcity conditions. Seven taro and eight sweet potato acc. from diverse geographical origins were evaluated for nitrogen (N), protein (Pt), starch (St), total mineral (M), calcium oxalate (CaOx), carbon isotope discrimination (Δ13C), and nitrogen isotopic composition (δ15N). Models were developed separately for both crops underground and aboveground organs. N, Pt, St, and M models could be used as quality control constituents, with a determination coefficient of prediction (r2pred) between 0.856 and 0.995. δ13C, δ15N, and CaOx, with r2pred between 0.178 and 0.788, could be used as an informative germplasm screening tool. The approach used in the present study demonstrates NIRS’s potential for further research on crop quality under drought.
Comparison of Cone Beam Computed Tomography and Digital Radiography in Detecting Separated Endodontic Files and Strip Perforation
Authors: Abdinian Mehrdad , Moshkforoush Saba , Hemati Hamidreza , Soltani Parisa , Moshkforoushan Mansoure , Spagnuolo Gianrico
Published: December 2020

The separation of endodontic files and strip perforation are among procedural intraoperative complications which may ultimately lead to the failure of root canal treatment. The aim of the present study was to compare the diagnostic potential of cone beam computed tomography (CBCT) and digital periapical radiographs in detecting separated rotary files and strip perforation in filled canals. Fifty human mandibular molars were selected for this study. The teeth were randomly divided into two groups based on endodontic errors (i.e., file separation and strip perforation). In each group, 25 of 50 mesial canals were randomly chosen for simulating the errors, while the other 25 canals were considered as the control group. In group one, a simulation of the separation of rotary files was performed using ProTaper F2 files. Strip perforation of the root canals in group two was achieved by number 2 and 3 Gates Glidden drills in the coronal third of the root canals. Digital periapical radiographs in two different horizontal angles and high-resolution CBCT scans were obtained from the teeth mounted on a dry human mandible with simulated soft tissue covering. Three experienced observers who were unaware of the study groups evaluated the digital periapical and CBCT image sets in two separate readings. Intraobserver and interobserver agreements, as well as accuracy, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV), were calculated and compared. Intraobserver and interobserver agreements ranged from poor to excellent and poor to good, respectively. The accuracy, sensitivity, specificity, PPV, and NPV for digital radiography in detecting separated files were 0.950, 0.813, 0.957, 0.929, and 0.880, respectively. The same values for CBCT were 0.747, 0.667, 0.900, 0.833, and 0.783, respectively. For the diagnosis of strip perforation, these values were 0.855, 0.800, 0.909, 0.889, and 0.833 for periapical radiography and 0.955, 1.000, 0.920, 0.926, and 1.000 for CBCT. In conclusion, CBCT was superior for diagnosing strip perforation of the filled root canals, while digital periapical radiographs performed better in the detection of separated rotary files.
Study on Mechanical and Viscoelastic Properties of Asphalt Mixture Modified by Diatomite and Crumb Rubber Particles
Authors: Liang Chunyu , Zhang Hao , Gu Zhengwei , Xu Xin , Hao Jinxin
Published: December 2020

To optimize the properties of asphalt mixtures and make full use of waste rubber tires, diatomite and crumb rubber particles were applied to reinforce the asphalt mixtures in this study. The rutting tests, the three-point bending tests, the freeze-thaw splitting tests, and the uniaxial compression creep tests were performed to analyze the effects of asphalt types and aggregate gradation on the pavement properties of diatomite and crumb rubber particles reinforced asphalt mixtures (DRPAM). Subsequently, the creep and relaxation characteristics of DRPAM were analyzed by the Burgers model, the modified Burgers model, the second-order extensive Maxwell model, and the Scott–Blair model. The results show that rubber particles and diatomite can reinforce the high temperature, low temperature, and viscoelastic properties of asphalt mixtures, although the improvement effect is weaker than styrene-butadiene-styrene (SBS). Consequently, it is concluded that rubber particle and diatomite compound modified asphalt mixture with suspension dense gradation and SBS binder will have better performance.
Rainwater Chemistry Reveals Air Pollution in a Karst Forest: Temporal Variations, Source Apportionment, and Implications for the Forest
Authors: Zeng Jie , Han Guilin
Published: December 2020

Temporal rainwater chemistry was used to reveal air pollution in the Maolan National Karst Forest Park (MNKFP), which is representative of the typical karst forest region of southwest China (SW China). The rainwater ions’ sources, variations, trends, and potential environmental effects were investigated from 2007 to 2010 and from 2013 to 2014. Based on the analysis of the temporal ionic concentrations of rainwater in the MNKFP, significant variations of ions were observed, including in NH4+ (9.7~266.6 μeq L−1) and SO42− (14.5~1396.4 μeq L−1), which were mainly controlled by variations in the source and rainfall amount; a decreased trend of rainwater pH was also observed. Accordingly, NH4+, Ca2+, SO42−, and Cl− were regarded as the most dominant ions. Typical ionic ratios and positive matrix factorization (PMF) model-based source apportionment suggested that anthropogenic inputs (coal combustion, industrial, traffic, and agricultural emissions) contributed 51% of F−, 93% of NO3−, 62% of SO42−, and 87% of NH4+, while the natural sources (crustal dust and sea salt) were the main sources of Cl− (74%), Na+ (82%), K+ (79%), Mg2+ (94%), and Ca2+ (93%). In combination with the reducing neutralization trend of temporal rainwater observed in the MNKFP and the potential effect of rainwater ion deposition on karst forests, more detailed monitoring of the rainfall-related deposition process is required for a better understanding of its potential environmental effects on the Earth’s surface.
A Study of the Effects of Anthropogenic Gaseous Emissions on the Microphysical Properties of Landfalling Typhoon Nida (2016) over China
Authors: Deng Lin , Gao Wenhua , Duan Yihong , Wu Chong
Published: December 2020

Using the Weather Research and Forecasting model with chemistry module (WRF-Chem), Typhoon Nida (2016) was simulated to investigate the effects of anthropogenic gaseous emissions on the vortex system. Based on the Multi-resolution Emission Inventory for China (MEIC), three certain experiments were conducted: one with base-level emission intensity (CTRL), one with one-tenth the emission of SO2 (SO2_C), and one with one-tenth the emission of NH3 (NH3_C). Results show that the simulations reasonably reproduced the typhoon’s track and intensity, which were slightly sensitive to the anthropogenic gaseous emissions. When the typhoon was located over the ocean, a prolonged duration of raindrop growth and more precipitation occurred in CTRL run. The strongest updraft in CTRL is attributed to the maximum latent heating through water vapor condensation. During the landfalling period, larger (smaller) differential reflectivities in the main-core of the vortex were produced in NH3_C (SO2_C) run. Such opposite changes of raindrop size distributions may lead to stronger (weaker) rainfall intensity, and the ice-related microphysical processes and the relative humidity in low troposphere were two possible influential factors. Moreover, additional ten-member ensemble results in which white noise perturbations were added to the potential temperature field, indicated that the uncertainty of thermodynamic field in the current numerical model should not be ignored when exploring the impacts of aerosol on the microphysics and TC precipitation.
Extended Atomic Structure Calculations for W11+ and W13+
Authors: Singh Narendra , Aggarwal Sunny , Mohan Man
Published: December 2020

We report an extensive and elaborate theoretical study of atomic properties for Pm-like and Eu-like Tungsten using Flexible Atomic Code (FAC). Excitation energies for 304 and 500 fine structure levels are presented respectively, for W11+ and W13+. Properties of the 4f-core-excited states are evaluated. Different sets of configurations are used and the discrepancies in identifications of the ground level are discussed. We evaluate transition wavelength, transition probability, oscillator strength, and collisional excitation cross section for various transitions. Comparisons are made between our calculated values and previously available results, and good agreement has been achieved. We have predicted some new energy levels and transition data where no other experimental or theoretical results are available. The present set of results should be useful in line identification and interpretation of spectra as well as in modelling of fusion plasmas.
Anaerobic Digestion in the 21st Century
Authors: Nikolausz Marcell , Kretzschmar Jörg
Published: December 2020

Despite being a mature biotechnological process, anaerobic digestion is still attracting considerable research attention, mainly due to its versatility both in substrate and product spectra, as well as being a perfect test system for the microbial ecology of anaerobes [...]
Comparative Analysis of Cell–Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models
Authors: Kutova Olga , Sencha Ludmila , Pospelov Anton , Dobrynina Olga , Brilkina Anna , Cherkasova Elena , Balalaeva Irina
Published: December 2020

Tumor resistance to therapy is associated with the 3D organization and peculiarities of the tumor microenvironment, of which intercellular adhesion is a key participant. In this work, the abundance of contact proteins was compared in SKOV-3 and SKOV-3.ip human ovarian adenocarcinoma cell lines, cultivated in monolayers, tumor spheroids and collagen hydrogels. Three-dimensional models were characterized by extremely low expression of basic molecules of adherens junctions E-cadherin and demonstrated a simultaneous decrease in desmosomal protein desmoglein-2, gap junction protein connexin-43 and tight junction proteins occludin and ZO-1. The reduction in the level of contact proteins was most pronounced in collagen hydrogel, accompanied by significantly increased resistance to treatment with doxorubicin and targeted anticancer toxin DARPin-LoPE. Thus, we suggest that 3D models of ovarian cancer, especially matrix-based models, tend to recapitulate tumor microenvironment and treatment responsiveness to a greater extent than monolayer culture, so they can be used as a highly relevant platform for drug efficiency evaluation.
Cutaneous Manifestations in Confirmed COVID-19 Patients: A Systematic Review
Authors: Conforti Claudio , Dianzani Caterina , Agozzino Marina , Giuffrida Roberta , Marangi Giovanni Francesco , di Meo Nicola , Morariu Silviu-Horia , Persichetti Paolo , Segreto Francesco , Zalaudek Iris , Neagu Nicoleta
Published: December 2020

There have been increasing reports of skin manifestations in COVID-19 patients. We conducted a systematic review and included manuscripts describing patients with positive RT-PCR coronavirus testing from nasopharyngeal swabs who also developed cutaneous manifestations. A total of 655 patients were selected, with different types of skin rashes: Erythematous maculopapular (n = 250), vascular (n = 146), vesicular (n = 99), urticarial (n = 98), erythema multiforme/generalized pustular figurate erythema/Stevens-Johnson syndrome (n = 22), ocular/periocular (n = 14), polymorphic pattern (n = 9), generalized pruritus (n = 8), Kawasaki disease (n = 5), atypical erythema nodosum (n = 3), and atypical Sweet syndrome (n = 1). Chilblain-like lesions were more frequent in the younger population and were linked to a milder disease course, while fixed livedo racemosa and retiform purpura appeared in older patients and seemed to predict a more severe prognosis. For vesicular rashes, PCR determined the presence of herpesviruses in the vesicle fluid, which raised the possibility of herpesvirus co-infections. The erythema-multiforme-like pattern, generalized pustular figurate erythema and Stevens-Johnson syndrome were most frequently linked to hydroxychloroquine intake. A positive PCR determination of SARS-COV-2 from conjunctival swabs suggest that eye discharge can also be contagious. These cutaneous manifestations may aid in identifying otherwise asymptomatic COVID-19 carriers in some cases or predict a more severe evolution in others.
Efficacy and Mode of Action of Mesenchymal Stem Cells in Non-Ischemic Dilated Cardiomyopathy: A Systematic Review
Authors: Hoeeg Cecilie , Frljak Sabina , Qayyum Abbas Ali , Vrtovec Bojan , Kastrup Jens , Ekblond Annette , Follin Bjarke
Published: December 2020

Non-ischemic dilated cardiomyopathy (NIDCM) constitutes one of the most common causes to non-ischemic heart failure. Despite treatment, the disease often progresses, causing severe morbidity and mortality, making novel treatment strategies necessary. Due to the regenerative actions of mesenchymal stem cells (MSCs), they have been proposed as a treatment for NIDCM. This systematic review aims to evaluate efficacy and mode of action (MoA) of MSC-based therapies in NIDCM. A systematic literature search was conducted in Medline (Pubmed) and Embase. A total of 27 studies were included (3 clinical trials and 24 preclinical studies). MSCs from different tissues and routes of delivery were reported, with bone marrow-derived MSCs and direct intramyocardial injections being the most frequent. All included clinical trials and 22 preclinical trials reported an improvement in cardiac function following MSC treatment. Furthermore, preclinical studies demonstrated alterations in tissue structure, gene, and protein expression patterns, primarily related to fibrosis and angiogenesis. Consequently, MSC treatment can improve cardiac function in NIDCM patients. The MoA underlying this effect involves anti-fibrosis, angiogenesis, immunomodulation, and anti-apoptosis, though these processes seem to be interdependent. These encouraging results calls for larger confirmatory clinical studies, as well as preclinical studies utilizing unbiased investigation of the potential MoA.
Exploring the Multifaceted Therapeutic Potential of Withaferin A and Its Derivatives
Authors: Behl Tapan , Sharma Aditi , Sharma Lalit , Sehgal Aayush , Zengin Gokhan , Brata Roxana , Fratila Ovidiu , Bungau Simona
Published: December 2020

Withaferin A (WA), a manifold studied, C28-steroidal lactone withanolide found in Withania somnifera. Given its unique beneficial effects, it has gathered attention in the era of modern science. Cancer, being considered a “hopeless case and the leading cause of death worldwide, and the available conventional therapies have many lacunae in the form of side effects. The poly pharmaceutical natural compound, WA treatment, displayed attenuation of various cancer hallmarks by altering oxidative stress, promoting apoptosis, and autophagy, inhibiting cell proliferation, reducing angiogenesis, and metastasis progression. The cellular proteins associated with antitumor pathways were also discussed. WA structural modifications attack multiple signal transduction pathways and enhance the therapeutic outcomes in various diseases. Moreover, it has shown validated pharmacological effects against multiple neurodegenerative diseases by inhibiting acetylcholesterinases and butyrylcholinesterases enzyme activity, antidiabetic activity by upregulating adiponectin and preventing the phosphorylation of peroxisome proliferator-activated receptors (PPARγ), cardioprotective activity by AMP-activated protein kinase (AMPK) activation and suppressing mitochondrial apoptosis. The current review is an extensive survey of various WA associated disease targets, its pharmacokinetics, synergistic combination, modifications, and biological activities.
Kahweol Ameliorates Cisplatin-Induced Acute Kidney Injury through Pleiotropic Effects in Mice
Authors: Kim Jung-Yeon , Jo Jungmin , Leem Jaechan , Park Kwan-Kyu
Published: December 2020

Cisplatin is an effective chemotherapeutic agent, but its clinical use is frequently limited by its nephrotoxicity. The pathogenesis of cisplatin-induced acute kidney injury (AKI) remains incompletely understood, but oxidative stress, tubular cell death, and inflammation are considered important contributors to cisplatin-induced renal injury. Kahweol is a natural diterpene extracted from coffee beans and has been shown to possess anti-oxidative and anti-inflammatory properties. However, its role in cisplatin-induced nephrotoxicity remains undetermined. Therefore, we investigated whether kahweol exerts a protective effect against cisplatin-induced renal injury. Additionally, its mechanisms were also examined. Administration of kahweol attenuated renal dysfunction and histopathological damage together with inhibition of oxidative stress in cisplatin-injected mice. Increased expression of nicotinamide adenine dinucleotide phosphate oxidase 4 and decreased expression of manganese superoxide dismutase and catalase after cisplatin treatment were significantly reversed by kahweol. Moreover, kahweol inhibited cisplatin-induced apoptosis and necroptosis in the kidneys. Finally, kahweol reduced inflammatory cytokine production and immune cell accumulation together with suppression of nuclear factor kappa-B pathway and downregulation of vascular adhesion molecules. Together, these results suggest that kahweol ameliorates cisplatin-induced renal injury via its pleiotropic effects and might be a potential preventive option against cisplatin-induced nephrotoxicity.
Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes, in Contrast to Adipose Tissue-Derived Stromal Cells, Efficiently Improve Heart Function in Murine Model of Myocardial Infarction
Authors: Stępniewski Jacek , Tomczyk Mateusz , Andrysiak Kalina , Kraszewska Izabela , Martyniak Alicja , Langrzyk Agnieszka , Kulik Klaudia , Wiśniewska Ewa , Jeż Mateusz , Florczyk-Soluch Urszula , Polak Katarzyna , Podkalicka Paulina , Kachamakova-Trojanowska Neli , Józkowicz Alicja , Jaźwa-Kusior Agnieszka , Dulak Józef
Published: December 2020

Cell therapies are extensively tested to restore heart function after myocardial infarction (MI). Survival of any cell type after intracardiac administration, however, may be limited due to unfavorable conditions of damaged tissue. Therefore, the aim of this study was to evaluate the therapeutic effect of adipose-derived stromal cells (ADSCs) and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) overexpressing either the proangiogenic SDF-1α or anti-inflammatory heme oxygenase-1 (HO-1) in a murine model of MI. ADSCs and hiPSCs were transduced with lentiviral vectors encoding luciferase (Luc), GFP and either HO-1 or SDF-1α. hiPSCs were then differentiated to hiPSC-CMs using small molecules modulating the WNT pathway. Genetically modified ADSCs were firstly administered via intracardiac injection after MI induction in Nude mice. Next, ADSCs-Luc-GFP and genetically modified hiPSC-CMs were injected into the hearts of the more receptive NOD/SCID strain to compare the therapeutic effect of both cell types. Ultrasonography, performed on days 7, 14, 28 and 42, revealed a significant decrease of left ventricular ejection fraction (LVEF) in all MI-induced groups. No improvement of LVEF was observed in ADSC-treated Nude and NOD/SCID mice. In contrast, administration of hiPSC-CMs resulted in a substantial increase of LVEF, occurring between 28 and 42 days after MI, and decreased fibrosis, regardless of genetic modification. Importantly, bioluminescence analysis, as well as immunofluorescent staining, confirmed the presence of hiPSC-CMs in murine tissue. Interestingly, the luminescence signal was strongest in hearts treated with hiPSC-CMs overexpressing HO-1. Performed experiments demonstrate that hiPSC-CMs, unlike ADSCs, are effective in improving heart function after MI. Additionally, long-term evaluation of heart function seems to be crucial for proper assessment of the effect of cell administration.
Lectin Protein as a Promising Component to Functionalize Micelles, Liposomes and Lipid NPs against Coronavirus
Authors: Alavi Mehran , Asare-Addo Kofi , Nokhodchi Ali
Published: December 2020

The outbreak of a novel strain coronavirus as the causative agent of COVID-19 pneumonia, first identified in Wuhan, China in December 2019, has resulted in considerable focus on virulence abilities of coronavirus. Lectins are natural proteins with the ability to bind specific carbohydrates related to various microorganisms, including viruses, bacteria, fungi and parasites. Lectins have the ability to agglutinate and neutralize these pathogeneses. The delivery of the encapsulated antiviral agents or vaccines across the cell membrane can be possible by functionalized micellar and liposomal formulations. In this mini-review, recent advances and challenges related to important lectins with inhibition activities against coronaviruses are presented to obtain a novel viewpoint of microformulations or nanoformulations by micellar and liposomal cell-binding carriers.
A Review of Accelerated Long-Term Forgetting in Epilepsy
Authors: Mameniškienė Rūta , Puteikis Kristijonas , Jasionis Arminas , Jatužis Dalius
Published: December 2020

Accelerated long-term forgetting (ALF) is a memory disorder that manifests by a distinct pattern of normal memory for up to an hour after learning, but an increased rate of forgetting during the subsequent hours and days. The topic of ALF has gained much attention in group studies with epilepsy patients and the phenomenon has been shown to have contradictory associations with seizures, epileptiform activity, imaging data, sleep, and antiepileptic medication. The aim of this review was to explore how clinical and imaging data could help determine the topographic and physiological substrate of ALF, and what is the possible use of this information in the clinical setting. We have reviewed 51 group studies in English to provide a synthesis of the existing findings concerning ALF in epilepsy. Analysis of recently reported data among patients with temporal lobe epilepsy, transient epileptic amnesia, and generalized and extratemporal epilepsies provided further indication that ALF is likely a disorder of late memory consolidation. The spatial substrate of ALF might be located along the parts of the hippocampal–neocortical network and novel studies reveal the increasingly possible importance of damage in extrahippocampal sites. Further research is needed to explore the mechanisms of cellular impairment in ALF and to develop effective methods of care for patients with the disorder.
Tau and DNA Damage in Neurodegeneration
Authors: Colnaghi Luca , Rondelli Diego , Muzi-Falconi Marco , Sertic Sarah
Published: December 2020

Neurodegenerative disorders are a family of incurable conditions. Among them, Alzheimer’s disease and tauopathies are the most common. Pathological features of these two disorders are synaptic loss, neuronal cell death and increased DNA damage. A key pathological protein for the onset and progression of the conditions is the protein tau, a microtubule-binding protein highly expressed in neurons and encoded by the MAPT (microtubule-associated protein tau) gene. Tau is predominantly a cytosolic protein that interacts with numerous other proteins and molecules. Recent findings, however, have highlighted new and unexpected roles for tau in the nucleus of neuronal cells. This review summarizes the functions of tau in the metabolism of DNA, describing them in the context of the disorders.
The Challenging Heterogeneity of Autism: Editorial for Brain Sciences Special Issue “Advances in Autism Research”
Authors: Narzisi Antonio
Published: December 2020

My personal experience as Guest Editor of the Special Issue (SI) entitled “Advances in Autism Research” began with a nice correspondence with Andrew Meltzoff, from the University of Washington, Seattle (WA, USA), which, in hindsight, I consider as a good omen for the success of this Special Issue: “Dear Antonio… [...]
Takt Planning in Apartment Building Renovation Projects
Authors: Keskiniva Kimmo , Saari Arto , Junnonen Juha-Matti
Published: December 2020

Takt production has been of great interest in construction during the last few years. In this research, a case study was carried out to demonstrate how the scheduling of an apartment building renovation project that utilizes takt production can be done. Furthermore, the study defines what clarifications should be made into the existing takt production models in the context of apartment building renovation projects, and it also explains why. Based on the study, adhering to a uniform production rate is challenging in apartment renovation projects. Therefore, a total of five clarifications to existing takt production methods are suggested. (1) Production with short takt requires a highly detailed definition of tasks in order to avoid ambiguousness. (2) Some tasks carried out in takt areas may have to be excluded from takt production. (3) The sensitivity of a created takt schedule should be evaluated, and buffers should be added accordingly. (4) Emphasis must be put on coordinating takted and non-takted tasks. (5) Takt plan modification during production requires effective procedures, since there is little time to react.
CFD Visualization in a Virtual Reality Environment Using Building Information Modeling Tools
Authors: Yan Jiayi , Kensek Karen , Konis Kyle , Noble Douglas
Published: December 2020

Scientific visualization has been an essential process in the engineering field, enabling the tracking of large-scale simulation data and providing intuitive and comprehendible graphs and models that display useful data. For computational fluid dynamics (CFD) data, the need for scientific visualization is even more important given the complicated spatial data structure and large quantities of data points characteristic of CFD data. To better take advantage of CFD results for buildings, the potential use of virtual reality (VR) techniques cannot be overlooked in the development of building projects. However, the workflow required to bring CFD simulation results to VR has not been streamlined. Building information modeling (BIM) as a lifecycle tool for buildings includes as much information as possible for further applications. To this end, this study brings CFD visualization to VR using BIM tools and reports the evaluation and analysis of the results.
Cancer Testis Antigens and Immunotherapy: Expression of PRAME Is Associated with Prognosis in Soft Tissue Sarcoma
Authors: Albertsmeier Markus , Altendorf-Hofmann Annelore , Lindner Lars H. , Issels Rolf D. , Kampmann Eric , Dürr Hans-Roland , Schubert-Fritschle Gabriele , Angele Martin K. , Kirchner Thomas , Jungbluth Achim A. , Knösel Thomas
Published: December 2020

(1) Background: PRAME, NY-ESO-1, and SSX2 are cancer testis antigens (CTAs), which are expressed in testicular germ cells with re-expression in numerous cancer types. Their ability to elicit humoral and cellular immune responses have rendered them promising targets for cancer immunotherapy, but they have never been studied in a large and well-characterised cohort of soft tissue sarcomas (STS). (2) Methods: On a protein level, we examined PRAME, NY-ESO-1, and SSX2 expression in tumour tissues of 249 high-risk STS using immunohistochemistry. We correlated expression levels with clinicopathological parameters including tumour-infiltrating lymphocyte (TIL) counts, grading, and long-term survival. (3) Results: Expression of PRAME, NY-ESO-1, and SSX2 was observed in 25 (10%), 19 (8%), and 11 (4%) of 249 specimens with distinct patterns for histo-subtypes. Expression of PRAME was associated with shorter patient survival (p = 0.005) and higher grade (G2 vs. G3, p = 0.001), while NY-ESO-1 expression was correlated with more favourable survival (p = 0.037) and lower grade (G2 vs. G3, p = 0.029). Both PRAME and NY-ESO-1 expression were more frequent in STS with low TIL counts. In multivariate analysis, high PRAME and low SSX2 expression levels as well as metastatic disease and non-radical resections were independent predictors of shorter overall survival. (4) Conclusions: CTAs PRAME, NY-ESO-1, and SSX2 show distinct expression patterns in different STS subtypes. These results demonstrate their prognostic relevance and may guide future immunotherapeutic approaches in STS.
Evaluation of Prognosticators and Treatment-Related Side Effects in Patients Irradiated Postoperatively for Endometrial Cancer
Authors: Leu Martin , Possiel Jacqueline , Schirmer Markus A. , Hille Andrea , Rieken Stefan , Dröge Leif Hendrik
Published: December 2020

Numerous clinical trials sought to improve outcomes in endometrial cancer patients with multimodal treatment strategies. We tested the hypothesis that specific histopathological and clinical parameters are prognosticators for outcomes at our Gynecological Cancer Center. A total of 203 patients (median age, 69.5 years) was included. They were irradiated postoperatively (n = 184: Brachytherapy, n = 19: Teletherapy) between 05/2007 and 03/2020. The median follow-up was 37.2 months. As statistical methods, we used the univariable Cox proportional hazards regression, and log-rank statistics. First, we found a significant influence of grading and nodal stage on outcomes. These findings underline the recommendations of more intense treatment in these patient groups, as already reflected in current guidelines. Secondly, we found that patient age had a significant influence on survival be it due to comorbidities and/or due to too hesitant treatment regimen in the elderly. Thus, it should be aimed at particular strategies in treatment of these patients. Lastly, we found very low rates of treatment-related side effects in patients treated with brachytherapy and moderate rates of side effects in patients treated with teletherapy. Overall, our study serves as basis for further improvement of treatment strategies and for conceptualization of clinical trials.
Chimeric Antigen Receptor (CAR)-Modified Immune Effector Cell Therapy for Acute Myeloid Leukemia (AML)
Authors: Acharya Utkarsh H. , Walter Roland B.
Published: December 2020

Despite the availability of an increasing number of targeted therapeutics and wider use of allogeneic hematopoietic stem cell transplantation, many patients with acute myeloid leukemia (AML) ultimately succumb to this disease. Given their remarkable efficacy in B-acute lymphoblastic leukemia and other CD19-expressing B cell malignancies, there is hope adoptive cellular transfer, particularly chimeric antigen receptor (CAR)-modified immune effector cell (IEC) therapies, may afford a novel, potent immune-based approach for the treatment of AML that complements or replaces existing ones and improves cure rates. However, it is unclear how best to translate the success of these therapies from B cell malignancies, where use of highly potent immunotherapies is facilitated by identified target antigens with near ubiquitous expression on malignant cells and non-fatal consequences from “on-target, off-tumor cell” toxicities. Herein, we review the current status of CAR-modified IEC therapies for AML, with considerations regarding suitable, relatively leukemia-restricted target antigens, expected toxicities, and interactions of the engineered cells with a profoundly immunosuppressive tumor microenvironment that restricts their therapeutic efficacy. With these challenges in mind, we will discuss possible strategies to improve the cells’ potency as well as their therapeutic window for optimal clinical use in AML.
Advances in Targeting Cancer-Associated Genes by Designed siRNA in Prostate Cancer
Authors: Bahreyni Amirhossein , Luo Honglin
Published: December 2020

Short interfering RNAs (siRNAs) have provided novel insights into the field of cancer treatment in light of their ability to specifically target and silence cancer-associated genes. In recent years, numerous studies focus on determining genes that actively participate in tumor formation, invasion, and metastasis in order to establish new targets for cancer treatment. In spite of great advances in designing various siRNAs with diverse targets, efficient delivery of siRNAs to cancer cells is still the main challenge in siRNA-mediated cancer treatment. Recent advancements in the field of nanotechnology and nanomedicine hold great promise to meet this challenge. This review focuses on recent findings in cancer-associated genes and the application of siRNAs to successfully silence them in prostate cancer, as well as recent progress for effectual delivery of siRNAs to cancer cells.