bluuebunny/crossref_metadata_2025 huggingface dataset:
Association Between Interpersonal Violence With Inadequate Nutritional Status Among Brazilian Adolescents
Authors: Marques Emanuele Souza , Hasselmann Maria Helena , de Barros Vianna Gabriela Vasconcellos , de Paula Mendonça Eliane , Azeredo Catarina Machado
Published: March 2021

Research focusing on the relationship between interpersonal violence and nutritional status in adolescence is scarce and has distinct results. The objective of this study is to investigate the association of family physical and sexual violence with inadequate nutritional status in Brazilian adolescents. We used data from the 2015 Brazilian National Survey of School Health. This study includes 11.850 students, older than 13 years, attending from sixth to ninth grade of elementary school and from the 1st to the 3rd year of high school. The exposures were family physical violence and rape. The outcome was nutritional status, assessed through body mass index. The association between exposures and outcome were investigated using a multinomial logistic regression model. These analyses were adjusted for demographic, socioeconomic, and family variables. The prevalence of family physical violence victimization was approximately 14% among adolescents for both sexes. The prevalence of rape was 4.6% and 5.7% among male and female adolescents, respectively. Family physical violence was not associated with being underweight, overweight, or obese, in either crude or adjusted models for both sexes. Sexual violence was inversely associated with being underweight only for male adolescents (OR: 0.21, CI 95%: 0.06–0.75). In female adolescents, sexual violence was associated with overweight/obesity (OR: 1.64, CI 95%:1.15–2.33). In this study, rape, but not family physical violence victimization, was associated with nutritional status in adolescents of both sexes. Nonetheless, this association was different between boys and girls. Rape was inversely associated with being underweight in male adolescents, whereas, in female adolescents, it was associated with excess body weight.
Preparation and physical characterization of cobalt iron oxide magnetic nanoparticles loaded polyvinyl alcohol
Authors: Abu-Abdeen M , Saber O , Mousa E
Published: March 2021

A solvent thermal method which depends on a thermal process under critical temperature and pressure was used to prepare cobalt iron oxide magnetic nanoparticles with a molar ratio 2. The prepared particles were in the form of nanoparticles with diameter ranging from 5 to 10 nm and with amorphous structure. Magnetic hysteresis behavior with saturation magnetization 36.31 emu/g and coercivity 4 Oe were observed for the nanoparticles. Polyvinyl alcohol was loaded with different concentrations of cobalt iron oxide nanoparticles using casting technique. Hysteresis loops for the polymer films were observed and both the saturation magnetization and coercivity were increased from 0.36 to 16.03 emu/g and 115 to 293 Oe for samples containing 5 and 20 wt% of nanoparticles, respectively. The elastic modulus of films was increased from 2.7 to 4.9 GPa for unloaded and loaded samples with 20 wt%, respectively. The storage modulus of the polymer films was found to obey the percolation behavior.
Countering Distrust in Illicit Online Networks: The Dispute Resolution Strategies of Cybercriminals
Authors: Dupont Benoît , Lusthaus Jonathan
Published: March 2021

The core of this article is a detailed investigation of the dispute resolution system contained within Darkode, an elite cybercriminal forum. Extracting the dedicated disputes section from within the marketplace, where users can report bad behavior and register complaints, we carry out content analysis on these threads. This involves both descriptive statistics across the data set and qualitative analysis of particular posts of interest, leading to a number of new insights. First, the overall level of disputes is quite high, even though members are vetted for entry in the first instance. Second, the lower ranked members of the marketplace are the most highly represented category for both the plaintiffs and defendants. Third, vendors are accused of malfeasance far more often than buyers, and their “crimes” are most commonly either not providing the product/service or providing a poor one. Fourth, the monetary size of the disputes is surprisingly small. Finally, only 23.1% of disputes reach a clear outcome.
Understanding the concept of compassion from the perspectives of nurses
Authors: Ortega-Galán Ángela María , Pérez-García Esteban , Brito-Pons Gonzalo , Ramos-Pichardo Juan Diego , Carmona-Rega María Inés , Ruiz-Fernández María Dolores
Published: March 2021

Background:

The high level of satisfaction of users of a health service is largely due to the fact that they receive excellent care from healthcare professionals. Compassionate care is an essential component of excellent care. But what do nurses understand compassion to be?

Research objectives:

To analyse the concept of compassion from the perspective of nurses in the Andalusian Public Health System, Spain.

Research design:

This is a qualitative study following the grounded theory model. Four focus groups and 25 in-depth interviews were conducted.

Participants and research context:

A total of 68 nursing professionals working in the Andalusian Public Health System (Spain) participated. Theoretical sampling was used, with participants being recruited using the snowball technique.

Ethical considerations:

This research was approved by the Research Ethics Committee of the Centro-Almería Health District (CEICA 27/9/17).

Findings:

From the analysis of the data, four themes emerged that helped to understand the concept of compassion according to nurses: ‘Negative perception of the term compassion’, ‘Compassion and empathy as synonyms’, ‘Beyond empathy’, and ‘Effects of having a compassionate attitude’.

Discussion:

Nurses perceive the concept of compassion differently to each other and even contradictorily. This concept is imbued with cultural elements, which adds confusion to understanding it, and is even perceived as something negative similar to pity.

Conclusion:

Nurses confuse the concepts of empathy and compassion as if they were synonymous. Before considering training in compassion for healthcare professionals, it is essential to clarify the concept of compassion through educational interventions.
Dens Invaginatus in Patients With Cleft Lip and Palate: A Case Series
Authors: Ickow Ilana M. , Zinn Steven , Stacy J. Matthew , Martin Brian , Losee Joseph E. , D’Alesio Alene , Soxman Jane , Schuster Lindsay A.
Published: March 2021

Dens invaginatus is a rare developmental anomaly characterized by an infolding of the enamel organ within the crown or root of a tooth, and it is an example of a dental anomaly that has a higher incidence in patients with CL/P. If undiagnosed, dens invaginatus can lead to severe, acute pain and pulpal necrosis since it can permit direct entry of bacteria into the dental pulp. Treatment of dens invaginatus includes prophylactic sealant or composite restoration, endodontic therapy if pulpal involvement has already occurred, or extraction if aberrant tooth morphology precludes endodontic therapy. Few studies report on the incidence of dens invaginatus in patients with CL/P. The purpose of this article is to describe 4 cases of dens invaginatus in patients with CL/P which were encountered in a cleft-craniofacial orthodontic clinic. Each case describes dens invaginatus in a maxillary lateral incisor, and treatments ranged from sealant application to endodontic therapy to extraction. These cases highlight the importance of awareness of this dental anomaly among cleft team providers to facilitate early diagnosis in patients with CL/P.
Examining Classism and Critical Consciousness Within Psychology of Working Theory
Authors: Kim Taewon , Allan Blake A.
Published: March 2021

Building from psychology of working theory, this study tested how critical consciousness, composed of perceived inequality, egalitarianism, and critical action, moderate the relations between contextual barriers (i.e., economic constraints and classism) and psychological variables (i.e., work volition and career adaptability) with a sample of 403 employees in the United States. Findings suggested that people who had high egalitarianism had a stronger negative relation between economic constraints and work volition. Results also revealed that people who had low egalitarianism had a negative relation between classism and career adaptability. Regarding critical action, people who had low or moderate levels of critical action had a stronger negative relation between economic constraints and work volition. Moreover, people who had low or moderate levels of critical action had a stronger negative relation between classism and career adaptability. Findings encourage practitioners and employers to consider egalitarianism and critical action as potential targets in vocational interventions.
How Gentle Must Violence Against Women Be in Order to Not Be Violent? Rethinking the Word “Violence” in Obstetric Settings
Authors: Lappeman Maura , Swartz Leslie
Published: March 2021

With the growing concern of human rights in health, the word “violence” is being used to describe apparent disrespectful treatment received by women by either health care practitioners or health care systems. As the definition of violence in health care settings broadens, questions arise over the impact of the term in describing objective reality. Specifically, does use of the term “violence” inadvertently disempower the women that it is meant to empower? This article explores the changing use of the term “violence” and specifically draws on evidence in a South African labor ward, where phenomena such as silence and limited social support have cultural underpinnings that contradict labels of structural or obstetric violence. As global research on obstetric violence in disadvantaged communities grows, choice of terminology will become more important in filtering results into medical policy and practice.
No (sociological) excuses for not going green: How do environmental activists make sense of social inequalities and relate to the working class?
Authors: Malier Hadrien
Published: March 2021

Some environmental activists occasionally use the argument that poverty is ‘no excuse’ for not going green and denounce discourses putting forward social conditions as unduly exculpatory. Employing participant observation among middle-class activists mobilising to diffuse environmental lifestyles in socially diverse suburbs near Paris (France), the article explores their relation to the working class and examines the consequences of their endeavours on local class relations. It describes the tension between their goal of mainstreaming environmental reflexivity and the stubborn existence of material inequalities and constraints. While their efforts are configured by a moral economy of environmental responsibility which assigns an undifferentiated moral obligation to consume sustainably to all individuals, they make sense of social differences by drawing on culturalist representations of poverty and folk social theories. These sense-making practices enhance rather than alleviate attributions of blame against working-class people and contribute to reinforcing the activists’ dominant symbolic position.
Understanding digital drug markets through the geography of postal drug deliveries in Scotland
Authors: Matthews Ben , Collier Ben , McVie Susan , Dibben Chris
Published: March 2021

An increase in the use of postal services to deliver drugs purchased online raises concerns about widening access to drugs markets, especially in remote and rural areas that were previously protected by geographical boundaries. Yet little is known about the geographical patterning of drugs delivered through the post. Using a novel law enforcement dataset containing details of illegal drug packages intercepted by UK Border Force en route to Scotland, we examine the geographical destination of drugs purchased online and explore the area-based characteristics associated with higher rates of delivery. This article provides previously unavailable insights into the spatial patterning of digital drugs markets at sub-national level. We use descriptive statistics, Bayesian hierarchical regression models, and spatial autocorrelation to describe the relationship between area-based characteristics and expected rate of illegal drug consignments identified across Scotland. The majority of intercepted drug packages were destined for urban centres, but there was a higher than expected delivery rate to some of Scotland’s remote and rural locations. Increased rates of drug delivery within Scottish neighbourhoods was independently associated with higher levels of crime and deprivation, with Internet connectivity and with access to services, but not with higher rates of drug-related hospitalization. Analysis of spatial clustering showed that drug delivery to the most remote and rural locations was still associated with good access to services because the packages were typically delivered to addresses in larger settlements within remote locations. Overall, postal drugs delivery reflects both relatively high use in more urban, more deprived areas but also seems to open up more remote regions to drug markets, albeit with usage concentrated in larger settlements within otherwise remote areas.
The Impact of Peer-Mentoring on the Academic Success of Underrepresented College Students
Authors: Venegas-Muggli Juan I. , Barrientos Carolina , Álvarez Fernando
Published: March 2021

This study evaluates the impact of the peer-mentoring program implemented by a Chilean higher education institution on underrepresented students’ academic success. Specifically, it assesses whether freshmen who enrolled in 2018 and took part in this initiative performed better than students with similar characteristics who did not. A quantitative quasi-experimental design was applied, using the Propensity Score Matching method. The results show that students who took part in this peer-mentoring program got better average grades and had better retention rates and attendance levels than those who did not. Strategies for developing successful mentoring initiatives for college students are discussed, with a special emphasis on their potential relevance to underrepresented students.
Electromagnetic shielding performance of copper and silver-plated hybrid yarn based multilayer fabrics in C & X band frequency range
Authors: Pandey Dharmendra Nath , Basu Arindam , Kumar Pramod
Published: March 2021

In the present study, a strategic designing of multilayer shield was planned to enhance the multiple reflection phenomenon to achieve maximum absorption properties in microwave frequency (C & X band) range. Multi-layer EMR shields were developed using pure cotton fabric and conductive woven fabrics, incorporated with copper- based & silver-plated hybrid yarn. First of all, single layer fabrics were produced in five variants, nomenclature as L1A (pure cotton) L1B, L1C (copper-based hybrid yarn), LS1B and LS1C (silver plated hybrid yarn). These five variants were used to prepare four sets of double & triple layer fabric. In both double and triple layer composition, L1A fabric (pure cotton) was used as top layer followed by B and C series fabrics, containing copper and silver-plated hybrid yarn. The EMSE performance in C and X band frequency range of single layer, double layer and triple layers in terms of scattering parameters S11(reflectance) & S21 (transmittance) in vertical and horizontal wave polarization was studied. It was found that number of layers, layer composition, orientation of metallic yarn, frequency and EM wave polarization have significant influence on overall electromagnetic shielding effectiveness.
Thalamus and Seizures—Here We Come Again…
Authors: Szaflarski Jerzy P.
Published: March 2021

[Box: see text]
Cafecitos Supporting Nurses in the Time of COVID-19: A Commentary From the NAHN-Westchester and NAHN–New York Chapters
Authors: Zavala Mirian , Crespo-Fierro Michele , Ortiz Caroline , Montoya Marisol , Rojas Patricia , Gonzalez Sylvia Collado , Gruber Debbie Ilarraza
Published: March 2021

Nurses have been called superheroes during this pandemic because of our compassion for our patients, but we need compassion, too. Through this state of emergency, quarantine, and isolation, the Cafecitos in the virtual world let us navigate this shared experience together.
Effects of Caffeine Supplementation on Physical Performance of Soccer Players: Systematic Review and Meta-Analysis
Authors: Ferreira Raphael Einsfeld Simões , Pacheco Rafael Leite , de Oliveira Cruz Latorraca Carolina , Riera Rachel , Eid Ricardo Guilherme , Martimbianco Ana Luiza Cabrera
Published: March 2021

Context:

Caffeine is 1 of the most popular supplements consumed by athletes, and the evidence for improving soccer performance remains limited.

Objective:

To investigate and update the effects (benefits and harms) of caffeine to improve performance on soccer players.

Data Sources:

Electronic search in Medline (via PubMed), CENTRAL, Embase, SPORTDiscus, and LILACS, from inception to March 28, 2020.

Study Selection:

Randomized clinical trials (RCTs) assessing the effects of caffeine on the performance of soccer players.

Study Design:

Systematic review with meta-analysis.

Level of Evidence:

Level 1.

Data Extraction:

Data extraction was conducted independently by 2 authors using a piloted form. We assessed methodological quality (Cochrane risk-of-bias [RoB] table) and the certainty of the evidence (GRADE [Grading of Recommendations Assessment, Development and Evaluation] approach).

Results:

Sixteen RCTs were included. Overall methodological quality was classified as unclear to low risk of bias. When assessing aerobic endurance, meta-analyses did not demonstrate the differences between caffeine and placebo (mean difference [MD], 44.9 m; 95% confidence interval [CI], −77.7 to 167.6). Similarly, no difference was observed during time to fatigue test (MD, 169.8 seconds; 95% CI, −71.8 to 411.6). Considering anaerobic power, meta-analyses also did not find differences for vertical jump (MD, 1.01 cm; 95% CI, −0.68 to 2.69) and repeated sprint tests (MD, −0.02 seconds; 95% CI, −0.09 to 0.04), as well as reaction time agility test (MD, 0.02 seconds; 95% CI, −0.01 to 0.04) and rating of perceived exertion (MD, 0.16 points; 95% CI, −0.55 to 0.87). Regarding safety, a few minor adverse events were reported. Based on the GRADE approach, the certainty of this evidence was classified as very low to low.

Conclusion:

We found no significant improvement in soccer-related performance with caffeine compared with placebo or no intervention. However, caffeine appears to be safe.
Multiparametric magnetic resonance imaging and positron emission tomography findings in neurodegenerative diseases: Current status and future directions
Authors: Soni Neetu , Ora Manish , Bathla Girish , Nagaraj Chandana , Boles Ponto Laura L , Graham Michael M , Saini Jitender , Menda Yusuf
Published: March 2021

Neurodegenerative diseases (NDDs) are characterized by progressive neuronal loss, leading to dementia and movement disorders. NDDs broadly include Alzheimer’s disease, frontotemporal lobar degeneration, parkinsonian syndromes, and prion diseases. There is an ever-increasing prevalence of mild cognitive impairment and dementia, with an accompanying immense economic impact, prompting efforts aimed at early identification and effective interventions. Neuroimaging is an essential tool for the early diagnosis of NDDs in both clinical and research settings. Structural, functional, and metabolic imaging modalities, including magnetic resonance imaging (MRI) and positron emission tomography (PET), are widely available. They show encouraging results for diagnosis, monitoring, and treatment response evaluation. The current review focuses on the complementary role of various imaging modalities in relation to NDDs, the qualitative and quantitative utility of newer MRI techniques, novel radiopharmaceuticals, and integrated PET/MRI in the setting of NDDs.
Embrace the Complex Dynamics of Twinning!
Authors: Cadée Franka M. , Nieuwenhuijze Marianne J. , Lagro-Janssen Antoine L. M. , de Vries Raymond
Published: March 2021

The professional growth of midwives, essential for optimizing midwifery leadership globally, can be enhanced through twinning collaborations. How twinning promotes growth is unclear. This case study explores how professional growth is affected by cultural differences between twins. We used a longitudinal qualitative design including data from open-ended questionnaires and focus groups. These data were analyzed using a content analysis. Our findings show that cultural differences were capable of both hindering and facilitating professional growth. Within the complex dynamics of twinning, professional growth was facilitated by twins’ preparedness to bridge cultural differences. Common goals positively influenced this process. Friction was more likely, and professional growth was hindered, when midwives were unprepared to bridge cultural differences. To optimize professional growth through twinning, we recommend a clear focus on common goals and consideration of the interaction between the length of a project and the extent of the cultural differences between twins.
Managerial Cognitive Bias, Business Transformation, and Firm Performance: Evidence From China
Authors: Wang Delu , Wang Yadong , Yang Jingyuan , Huang Ziyang , Cui Rong
Published: March 2021

Business transformation has become an important way of sustainable development of enterprises. However, the transformation performance is not ideal. Using data from Chinese listed companies from 2001 to 2016, this article employs the Heckman selection model to explore the impact of managerial cognitive bias on transformation strategy and firm performance. The results demonstrate that managerial cognitive bias plays an important role in inducing business transformation. The higher the degree of managerial overconfidence and overoptimism, the more the enterprise will tend to implement business transformation. An important contribution of the article is that it reveals the significant difference between overconfidence and overoptimism: The more overconfident the managers are, the more likely they are to adopt both internal cultivation and mergers and acquisitions (M&A) to realize firm business transformation, whereas the more overoptimistic the managers are, the more likely they are to adopt M&A rather than internal cultivation to realize firm business transformation. Furthermore, business transformation conducted by overconfident managers helps improve firm financial performance and market value, while transformation conducted by overoptimistic managers helps reduce both.
The Gantry Crane Technique: A Novel Technique for Treating Severe Thoracic Spinal Stenosis and Myelopathy Caused by Ossification of the Ligamentum Flavum and Preliminary Clinical Results
Authors: Zhu Jian , Luo Xi , Sun Kaiqiang , Sun Jingchuan , Wang Yuan , Xu Ximing , Shi Jiangang
Published: March 2021

Study Design:

Retrospective single-arm study.

Objective:

To propose a novel technique named the gantry crane technique for treating severe thoracic spinal stenosis and myelopathy caused by thoracic ossification of the ligamentum flavum (TOLF) and investigate its clinical results.

Methods:

From June 2017 to January 2019, 18 patients presenting with severe spinal stenosis and myelopathy caused by TOLF were included in our study. All patients were treated with gantry crane technique, pre-operative JOA score, as well as 3 days-, 3 months-, 6 months-, 12 months-, 24 months after operation, and Hirabayashi recovery rate were reported. Pre- and post-operative image were utilized for the assessment of post-operative effect. Peri-operative complications were recorded to assess the safety of the gantry crane technique.

Results:

The JOA score increased from 10.56 ± 3.76 preoperatively to 12.94 ± 3.33, 13.56 ± 3.48, 13.94 ± 3.32, 14.17 ± 3.70 and 14.06 ± 3.54 in 3 days, 3 months, 6 months, 12 months and 24 months after surgery, respectively. The post-operative JOA scores were improved with statistical significance at the level of P < 0.05. The recovery rate was (39.09 ± 33.85) %, (51.35 ± 42.60) %, (55.79 ± 36.10) %, (64.98 ± 29.24) % and (60.98 ± 35.96) % for 3 days, 3 months, 6 months, 12 months and 24 months after surgery, respectively. There were 2 cases of SSI (surgical site infection), 1 case of NI (neurovascular injury) and 1 case of cerebrospinal fluid (CSF) leakage.

Conclusions:

This study highlights a safe and effective technique, the gantry crane technique, for treating severe thoracic spinal stenosis and myelopathy caused by TOLF.
Outcomes of Plantaris Tendon Augmentation in Acute Achilles Tendon Rupture Repair
Authors: Rogero Ryan G. , Corr Daniel , Fisher Andrew , O’Neil Joseph T. , Fuchs Daniel J. , Raikin Steven M.
Published: March 2021

Category:

Trauma; Ankle; Hindfoot; Sports

Introduction/Purpose:

Given the lack of consensus regarding the optimal surgical treatment for acute Achilles tendon ruptures, identifying techniques to maximize functional outcomes is crucial. Augmentation of Achilles tendon repair with the plantaris tendon is an established surgical technique that is thought to improve outcomes by reinforcing the repair site as well as decreasing soft tissue adhesions, though no studies have specifically looked at outcomes of its use. The purpose of this study is to compare the outcomes of acute Achilles tendon repairs managed with and without plantaris tendon augmentation.

Methods:

A retrospective review of patients undergoing open repair of acute Achilles tendon ruptures with a single fellowship- trained foot & ankle surgeon from 2010-2016 was performed. Patients <18 years of age, those whose procedures were >21 days from the date of injury, or whose repair was augmented with a flexor hallucis longus (FHL) tendon transfer or V-Y advancement were excluded. Operative report review determined if repair included plantaris augmentation. Augmentation involved weaving the tendon through the Achilles above and below the rupture site creating a box type biologic augmentation. Patients were contacted at >=2 years postoperatively to complete the Foot & Ankle Ability Measure (FAAM)-Activities of Daily Living (ADL) and -Sports subscales, visual analog scale (VAS) for pain, as well as surveyed regarding their satisfaction with the surgical outcome using a 5- point Likert scale. Mann-Whitney U, Chi-squared and Fisher’s exact tests were performed to compare patient variables and postoperative outcomes.

Results:

One hundred twenty-eight patients undergoing repair of an acute Achilles tendon rupture were included, including 59 (46.1%) with plantaris tendon augmentation and 69 (53.9%) without. The two patient cohorts did not differ in any preoperative patient variables (p>0.0892). Patients with and without plantaris augmentation did not experience any significant difference in the rate of postoperative complications (p=1.000), including a 0% re-rupture rate in each group. Additionally, at an average follow-up of 57.1 months, the groups did not differ in FAAM-ADL score (p=0.7116), FAAM-Sports score (p=0.4024), the Single Assessment Numeric Evaluation (SANE) as part of the FAAM-Sports subscale (p=0.2572), VAS pain (p=0.1885), or outcome satisfaction (p=0.7317).

Conclusion:

Our study demonstrates that plantaris tendon augmentation does not improve patient functional outcomes following acute Achilles tendon repair, but also is not associated with increased complication rates. Further studies, including either higher-level prospective, randomized clinical studies or biomechanical evaluation of the technique, are indicated to justify this adjunctive procedure. [Table: see text]
First-in-human phase 1 trial of induced regulatory T cells for graft-versus-host disease prophylaxis in HLA-matched siblings
Authors: MacMillan Margaret L. , Hippen Keli L. , McKenna David H. , Kadidlo Diane , Sumstad Darin , DeFor Todd E. , Brunstein Claudio G. , Holtan Shernan G. , Miller Jeffrey S. , Warlick Erica D. , Weisdorf Daniel J. , Wagner John E. , Blazar Bruce R.
Published: March 2021

Human CD4+25− T cells cultured in interleukin 2 (IL-2), rapamycin, and transforming growth factor β (TGFβ) along with anti-CD3 monoclonal antibody–loaded artificial antigen-presenting cells generate FoxP3+ induced regulatory T cells (iTregs) with potent suppressive function. We performed a phase 1, single-center, dose-escalation study to determine the safety profile of iTregs in adults with high-risk malignancy treated with reduced-intensity conditioning and mobilized peripheral blood stem cells (PBSCs) from HLA-identical sibling donors. Sixteen patients were enrolled and 14 were treated (2 productions failed to meet desired doses). One patient each received 3.0 × 106/kg, 3.0 × 107/kg, and 3.0 × 108/kg iTregs with corresponding T-conventional-to-iTreg ratios of 86:1, 8:1, and 1:2. After 3 patients received 3.0 × 108/kg in the presence of cyclosporine (CSA) and mycophenolate mofetil (MMF) with no dose-limiting toxicities, subsequent patients were to receive iTregs in the presence of sirolimus/MMF that favors Foxp3 stability based on preclinical modeling. However, 2 of 2 developed grade 3 acute graft-versus-host disease (GVHD), resulting in suspension of the sirolimus/MMF. An additional 7 patients received 3.0 × 108/kg iTregs with CSA/MMF. In the 14 patients treated with iTregs and CSA/MMF, there were no severe infusional toxicities with all achieving neutrophil recovery (median, day 13). Of 10 patients who received 3.0 × 108/kg iTregs and CSA/MMF, 7 had no aGVHD, 2 had grade 2, and 1 had grade 3. Circulating Foxp3+ iTregs were detectable through day 14. In summary, iTregs in the context of CSA/MMF can be delivered safely at doses as high as 3 × 108/kg. This trial was registered at www.clinicaltrials.gov as #NCT01634217.
Gene expression profile correlates with molecular and clinical features in patients with myelofibrosis
Authors: Rontauroli Sebastiano , Castellano Sara , Guglielmelli Paola , Zini Roberta , Bianchi Elisa , Genovese Elena , Carretta Chiara , Parenti Sandra , Fantini Sebastian , Mallia Selene , Tavernari Lara , Sartini Stefano , Mirabile Margherita , Mannarelli Carmela , Gesullo Francesca , Pacilli Annalisa , Pietra Daniela , Rumi Elisa , Salmoiraghi Silvia , Mora Barbara , Villani Laura , Grilli Andrea , Rosti Vittorio , Barosi Giovanni , Passamonti Francesco , Rambaldi Alessandro , Malcovati Luca , Cazzola Mario , Bicciato Silvio , Tagliafico Enrico , Vannucchi Alessandro M. , Manfredini Rossella
Published: March 2021

Myelofibrosis (MF) belongs to the family of classic Philadelphia-negative myeloproliferative neoplasms (MPNs). It can be primary myelofibrosis (PMF) or secondary myelofibrosis (SMF) evolving from polycythemia vera (PV) or essential thrombocythemia (ET). Despite the differences, PMF and SMF patients are currently managed in the same way, and prediction of survival is based on the same clinical and genetic features. In the last few years, interest has grown concerning the ability of gene expression profiles (GEPs) to provide valuable prognostic information. Here, we studied the GEPs of granulocytes from 114 patients with MF, using a microarray platform to identify correlations with patient characteristics and outcomes. Cox regression analysis led to the identification of 201 survival-related transcripts characterizing patients who are at high risk for death. High-risk patients identified by this gene signature displayed an inferior overall survival and leukemia-free survival, together with clinical and molecular detrimental features included in contemporary prognostic models, such as the presence of high molecular risk mutations. The high-risk group was enriched in post-PV and post-ET MF and JAK2V617F homozygous patients, whereas pre-PMF was more frequent in the low-risk group. These results demonstrate that GEPs in MF patients correlate with their molecular and clinical features, particularly their survival, and represent the proof of concept that GEPs might provide complementary prognostic information to be applied in clinical decision making.
SDG711 Is Involved in Rice Seed Development through Regulation of Starch Metabolism Gene Expression in Coordination with Other Histone Modifications
Authors: Liu Xiaoyun , Luo Junling , Li Tiantian , Yang Huilan , Wang Ping , Su Lufang , Zheng Yu , Bao Chun , Zhou Chao
Published: March 2021

SDG711 is a histone H3K27me2/3 transmethylase in rice, a homolog of CLF in Arabidopsis, and plays key roles in regulating flowering time and panicle development. In this work, we investigated the role of SDG711 in rice seed development. Overexpression and downregulation of SDG711 lead to a decrease and increase in the expression level of genes related to starch accumulation, resulting in smaller seeds or even seed abortion. ChIP assay showed that SDG711-mediated H3K27me3 changed significantly in genes related to endosperm development, and SDG711 can directly bind to the gene body region of several starch synthesis genes and amylase genes. In addition, H3K4me3 and H3K9ac modifications also cooperate with H3K27me3 to regulate the development of the endosperm. Our results suggest that the crosstalk between SDG711-mediated H3K27me3 and H3K4me3, and H3K9ac are involved in starch accumulation to control normal seed development.
Fate of recycled tyre granulate used on artificial turf
Authors: Verschoor Anja J. , van Gelderen Alex , Hofstra Ulbert
Published: March 2021

The production of granulates as infill for artificial turf is able to process 21% of the end-of-life tyres in Europe, approximately 600 million kg per year. In doing so it avoids an annual CO2 emission comparable with the amount that could be absorbed by around 30 km2 of forest. However, dispersal of rubber infill to the environment is perceived as a problem. An amount of 3000–5000 kg granulate per field per year is currently used as underpinning for a European proposal to ban rubber infill as part of the intended restriction on intentionally added microplastics in 2021. By reviewing grey research reports, we found out that the dispersal rates are based on the false assumption that the annual granulate demand for refilling is necessary because of granulate losses to the environment. However, it has been ignored that part of the refill is needed because the infill layer settles and becomes more dense (compaction) and that part of the lost infill is collected and reused on the fields. In combination with unawareness and improper piling of snow in the past, these are the causes of the high estimates of infill dispersal per year. This paper shows the current state-of-knowledge about ELT granulate dispersal and shows that approximately 600–1200 kg refill is required annually to compensate for compaction and for some infill waste on pavements and in drainage sinks. Recommended mitigation measures are containment through optimized field and drainage construction, suitable maintenance equipment and practices and good-housekeeping rules for players and groundkeepers and handling end-of-life pitches. If these recommendations are implemented, the emission of ELT granulates to the environment can be reduced to virtually zero.
Central neck lymph node metastasis in oral squamous cell carcinoma at the floor of mouth
Authors: Zhang Songtao , Zhang Runfang , Wang Chao , Gong Wenbo , Xue Miaomiao , Liu Lina , Zhang Yuanyuan
Published: March 2021

Abstracts

Background

Our goal was to analyze the incidence of level VI metastasis in previously untreated oral squamous cell carcinoma (SCC) patients and their clinicopathological and prognostic characteristics.

Methods

Oral SCC patients with level VI metastasis were retrospectively enrolled, and their demographic and pathologic features as well as their survival data were descriptively analyzed.

Results

A total of 13 cases from 1875 patients were included, all patients had SCC at the floor of mouth (SCCFOM). Eight (61.5%) patients had a pT4 tumor, and all patients had a pathological N3 neck with multiple positive lymph nodes. Adverse pathologic features were present in 100% of the patients. The size of the metastatic foci in level VI ranged from 2.6 cm to 4.5 cm with a mean value of 3.2 cm, and 5 patients showed a soft tissue deposit with no lymph node component. Recurrence occurred in all patients, and 11 patients died of uncontrolled cancer within 5 years after surgery.

Conclusion

Level VI metastasis in primary oral SCCFOM is rare, and its prognosis is poor.
Bmi-1 alleviates adventitial fibroblast senescence by eliminating ROS in pulmonary hypertension
Authors: Li Kai , Li Yan , Yu Youjia , Ding Jingjing , Huang Huijie , Chu Chunyan , Hu Li , Yu Yanfang , Cao Yue , Xu Peng , Fulton David , Chen Feng
Published: March 2021

Objectives

Pulmonary hypertension (PH) is a life-threatening progressive disease with high mortality in the elderly. However, the pathogenesis of PH has not been fully understood and there is no effective therapy to reverse the disease process. This study aims to determine whether cellular senescence is involved in the development of PH.

Methods

The rat PH model was established by intraperitoneal injection of monocrotaline and evaluated by pulmonary arteriole wall thickness and right ventricular hypertrophy index. Human lung fibroblasts (HLFs) were treated with CoCl2 or hypoxia to induce cellular senescence in vitro. SA-β-gal staining and the changes of senescent markers were used to examine cellular senescence. The molecular mechanism of cellular senescence was further explored by detecting reactive oxygen species (ROS) levels and culturing cells with a conditioned medium.

Results

We revealed the cellular senescence of pulmonary adventitial fibroblasts in vivo in the rat PH model. The expression of Bmi-1, an important regulator of senescence, was decreased in the lungs of PH rats and localized in adventitial fibroblasts. The in vitro experiments showed that p16 expression was increased while Bmi-1 expression was decreased after CoCl2 treatment in HLFs. Mechanistically, Bmi-1 could alleviate CoCl2-induced HLFs senescence by eliminating ROS which further promoted the proliferation of pulmonary artery smooth muscle cells by paracrine mode of action of HLFs.

Conclusion

Bmi-1 alleviates the cellular senescence of pulmonary fibroblasts in PH, which expands the pathogenesis of PH and provides a theoretical basis for targeting senescent cells in the treatment of PH.
Neuropsychiatric, cognitive and sexual impairment in mastocytosis patients
Authors: Jendoubi Fatma , Severino-Freire Maella , Negretto Mathilde , Arbus Christophe , Paul Carle , Bulai Livideanu Cristina
Published: March 2021

Background

Mastocytosis is a rare disease characterised by the accumulation and/or proliferation of abnormal mast cells (MCs) in one or several organs. It may present with a number of different symptoms that involve various organ systems. The current study aims to assess the prevalence of MC mediator-related symptoms in a cohort of mastocytosis patients with a specific focus on neurological, psychiatric, cognitive and sexual symptoms. We also assessed the impact of the disease on patients’ professional lives. Patients were administered a validated multidimensional questionnaire to collect information on patients’ perception of the severity of their symptoms. From the questionnaires we extracted the neurological, cognitive, psychiatric and sexual symptoms and the impact of the disease on patients’ professional lives as well as their grading. The affective status was assessed using the 17-item version of the Hamilton Depression Rating Scale.

Results

We included 139 patients. Mastocytosis was classified as systemic in 113 patients and cutaneous in 26 patients. The prevalence of MC mediator-related systemic symptoms was as follows: cutaneous (71%), gastro-intestinal (48%), cardio-vascular (36%), musculoskeletal (26.6%), fatigue (24%), urinary (14.4%) and respiratory (10%). Headaches and vertigo were noted in respectively 55% and 32% of patients. Irritability, episodes of memory loss and difficulty concentrating were reported in 54%, 52% and 40% of cases, respectively. Sexual impairment was noted in 24% of patients. No associations were found between neuropsychiatric/cognitive impairment and age, gender, diagnostic delay, disease form, the presence of cutaneous lesions, the level of serum and bone marrow tryptase and the presence of KIT mutation in bone marrow and/or skin. Depression was noted in 49% of patients. One in four patients reported a negative impact of the disease on their professional lives.

Conclusion

This current study provides some insights regarding symptoms related to mastocytosis and their impact on patients’ professional lives.
Microdialysis for chronic exertional compartment syndrome: a pilot study
Authors: Lohrer Heinz , Klein Jochen , Nauck Tanja , Schönberg Tobias
Published: March 2021

Background

Diagnosing chronic exertional compartment syndrome (CECS) is still a challenge. An increase in intramuscular pressure during and following exercise is accepted as the diagnostic standard. However, neither the methods used nor the interpretation of the obtained results are sufficiently standardized.

Methods

In the present pilot study, the metabolic state of CECS patients was investigated using microdialysis. We hypothesized that there was no difference in intramuscular concentrations of glucose, lactate, glutamate, and glycerol before and after exercise (H10) or between patients suffering from CECS and healthy control subjects (H20). This study was designed as an explorative case-control study (level of evidence III). Twelve patients suffering from CECS of the lower leg and six matched asymptomatic control subjects underwent microdialysis in the anterior (n = 7) or deep posterior compartment (n = 11) of the leg. Following ultrasound-guided insertion of the microdialysis catheters, 10-minute fractions of the dialysates were collected first during rest and then following fatigue- or pain-induced discontinuation of exercise. Dialysates were analysed for lactate, glucose, glutamate, and glycerol concentrations 6 × 10 min before and 6 × 10 min after exercise.

Results

Exercise-induced increases in lactate, glutamate, and glycerol concentrations were detected in both CECS patients and control subjects (all p < 0.001). No differences between CECS patients and control subjects were found by comparing the intramuscular glucose, lactate, glutamate, and glycerol concentrations at rest and following exercise (all p > 0.05).

Conclusions

We found exercise-induced increases in the lactate, glutamate, and glycerol levels in skeletal muscle. However, the metabolic changes did not differentiate CECS patients from healthy subjects.

Trial registration

The registration trial number is DRKS00021589 on DRKS. ‘Retrospectively registered’. Date of registration: April 4, 2020.
Atezolizumab in combination with intrathecal chemotherapy and radiation for treatment of isolated cerebral nervous system relapse in a patient with extranodal NK/T cell lymphoma: a case report
Authors: Lipsitt Amanda E. , Hung Jaclyn Y. , Langevin Anne-Marie
Published: March 2021

Background

Extranodal NK/T cell lymphoma (ENKTL) is an aggressive form of Epstein-Barr virus (EBV)-associated non-Hodgkin’s lymphoma which historically has a poor prognosis. When relapse occurs, particularly in the cerebral nervous system (CNS), survival is rare. The immune checkpoint pathway family of proteins is highly expressed in many human tumors, especially in EBV-related malignancies. To the best of our knowledge, there are no reports of immune checkpoint inhibitors used either alone or in combination for the treatment of ENTKL CNS relapse, yet there are promising results in metastatic CNS involvement of other malignancies.

Case presentation

This is the case of a 29-year-old Hispanic male with ENKTL who was treated at first relapse with 24 doses of the programmed death-ligand 1 (PD-L1) immune checkpoint inhibitor, atezolizumab, over a 17-month period. He remained in remission for 18 months until he experienced an isolated CNS relapse and on-going evidence of chronic EBV infection. Salvage therapy was provided as a combination of triple intrathecal (TIT) chemotherapy, radiation, and atezolizumab. He continues on maintenance atezolizumab and remains alive 1-year post CNS relapse.

Conclusions

The results from this case suggest that atezolizumab should be considered as part of the treatment regimen for relapsed ENKTL. They also demonstrate the benefit of using atezolizumab in combination with TIT chemotherapy and radiation as a viable treatment option for ENKTL CNS relapse and indicate that atezolizumab is an option for long-term maintenance therapy for patients with ENKTL.
A proposed modulatory role of the endocannabinoid system on adipose tissue metabolism and appetite in periparturient dairy cows
Authors: Myers Madison N. , Zachut Maya , Tam Joseph , Contreras G. Andres
Published: March 2021

To sustain the nutrient demands of rapid fetal growth, parturition, and milk synthesis, periparturient dairy cows mobilize adipose tissue fatty acid stores through lipolysis. This process induces an inflammatory response within AT that is resolved as lactation progresses; however, excessive and protracted lipolysis compounds the risk for metabolic and inflammatory diseases. The suppression of lipolytic action and inflammation, along with amplification of adipogenesis and lipogenesis, serve as prospective therapeutic targets for improving the health of periparturient dairy cows. Generally, the activation of cannabinoid receptors by endocannabinoids enhances adipogenesis and lipogenesis, suppresses lipolysis, and increases appetite in mammals. These biological effects of activating the endocannabinoid system open the possibility of harnessing the endocannabinoid system through nutritional intervention in dairy herds as a potential tool to improve dairy cows’ health, although much is still to be revealed in this context. This review summarizes the current knowledge surrounding the components of the endocannabinoid system, elaborates on the metabolic effects of its activation, and explores the potential to modulate its activity in periparturient dairy cows.
Multivariate distributions of correlated binary variables generated by pair-copulas
Authors: Lin Huihui , Chaganty N. Rao
Published: March 2021

Correlated binary data are prevalent in a wide range of scientific disciplines, including healthcare and medicine. The generalized estimating equations (GEEs) and the multivariate probit (MP) model are two of the popular methods for analyzing such data. However, both methods have some significant drawbacks. The GEEs may not have an underlying likelihood and the MP model may fail to generate a multivariate binary distribution with specified marginals and bivariate correlations. In this paper, we study multivariate binary distributions that are based on D-vine pair-copula models as a superior alternative to these methods. We elucidate the construction of these binary distributions in two and three dimensions with numerical examples. For higher dimensions, we provide a method of constructing a multidimensional binary distribution with specified marginals and equicorrelated correlation matrix. We present a real-life data analysis to illustrate the application of our results.