bluuebunny/crossref_metadata_2025 huggingface dataset:
PSV-15 Effects of Supplementation of Protease to Different Nutrient Density of Diets on Growth Performance, Nutrient Digestibility, Blood Profile, Gas Emission, Fecal Microflora in Growing-Finishing Pig
Authors: Park Sehyun , Oh Hanjin , Kim Yongju , An Jaewoo , Chang Seyeon , Go Youngbin , Cho Hyunah , Song Dongcheol , Jeon Kyeong Ho , Hong Seok Man , Yun Won , Cho Jinho
Published: October 2023

This study was conducted to investigate the effects of protease supplementation and different nutrient density of diets on growth performance, nutrient digestibility, blood profiles, gas emissions, and fecal microflora in growing-finishing pigs. Crossbred growing pigs (n = 180; [Landrace × Yorkshire] x Duroc) with an initial body weight (BW; 18.74 ± 3.46 kg) were used for 15 weeks. Pigs were randomly assigned to 6 dietary treatments with 6 replicates of 3 pigs per pen in a 3 ⅹ 2 factorial through the following arrangement: Three groups of protease (1, Basal diets; 2, Protease A: 125 mg/kg protease derived from Streptomyces spp; 3, Protease B: 100 mg/kg protease derived from Bacillus licheniformis) at two different nutrient density diets (1; Basal requirement 2; 0.94-0.98% greater than requirement in dietary protein and 50 kcal/kg in energy). Supplementation of protease significantly increased (P < 0.05) BW at weeks 4 and 6. Also, supplementation of protease increased (P < 0.05) ADG at grower periods and weeks 13 to 15. In addition, improvement (P < 0.05) of ADG was observed in pigs fed a high nutrient density diet (HN) compared with basal nutrient density diet (BN) at grower periods and weeks 13 to 15. In apparent total track digestibility (ATTD) of crude protein (CP), there was increased (P < 0.05) in supplementation of protease at weeks 6 and 13. The HN showed increased (P < .0001) ATTD of CP at 6 weeks. BN increased (P < 0.01) ATTD of CP at week 13. Also, there was interaction (P < 0.05) between nutrient density of diets and supplementation of protease. In ATTD of gross energy, supplementation of protease showed increased (P < 0.05) at week 13. The supplementation of protease in diets decreased (P < 0.05) the concentration of blood urea nitrogen (BUN) at week 13. The supplementation of protease in diets decreased (P < 0.05) the emission of NH3 at weeks 6 and 13. Also, H2S was decreased (P < 0.05) due to supplementation of Protease A in diets. At weeks 6 to 9, average daily feed intake showed a tendency to increase (P < 0.10) when the BN diets were fed. Also, during the entire period, feed conversion ratio tended to increase (P < 0.10) when the BN diets were fed. In conclusion, supplementation of protease reduces NH3 in feces and BUN in whole blood by increasing the digestibility of CP and improved growth performance. Also, diets with high nutrient density improved growth performance and nutrient digestibility in grower periods.
PSV-18 Effects of Replacing Lactose with Novel Carbohydrate Sources on Nursery Pig Growth Performance
Authors: Royall Rafe Q , Woodworth Jason C , Tokach Mike D , DeRouchey Joel M , Gebhardt Jordan T , Goodband Robert D , Mertz Keith , Patience John
Published: October 2023

This experiment was conducted to determine the effects of replacing lactose in Phase 1 and 2 nursery diets with 1 of 2 novel carbohydrate (CHO) products (CHO-D and CHO-L; Cargill Starches, Sweeteners, & Texturizers, Blair, Nebraska) on growth performance and fecal dry matter. A total of 360 barrows (DNA 200×400; initially 6.0 ± 0.04 kg) were used in a 42-d growth trial. Pigs were weaned at approximately 21 d of age, randomly allotted to pens in 1 of 2 body weight (BW) blocks based on initial BW (initially 5.4 and 6.6 kg), and then allotted to 1 of 6 dietary treatments in completely randomized design, with 5 pigs/pen and 12 replications across 2 barns. Dietary treatments were corn-soybean meal- based with 5 to 7.5% DDGS and included: 1) negative control (NC; containing 0.08 and 0.04% lactose, phase 1 and 2, respectively); 2) positive control (PC; containing 10 and 5% lactose, phase 1 and 2, respectively); 3) 50% of lactose replaced with the dry novel CHO (50% CHO-D; containing 5 and 2.5% lactose, phase 1 and 2, respectively); 4) 100% of lactose replaced with CHO-D (100% CHO-D; containing 0.09 and 0.05% lactose, phase 1 and 2 respectively); 5) 50% of lactose replaced with the liquid novel CHO (50% CHO-L; containing 5 and 2.5% lactose, phase 1 and 2, respectively); or 6) 100% of lactose replaced with CHO-L (100% CHO-L; containing 0.09 and 0.05% lactose, phase 1 and 2 respectively). Novel CHO sources (CHO-D or CHO-L) replaced whey powder on a carbohydrate basis in Phase 1 and 2 diets. Treatment diets were formulated in two phases fed from d 0 to 10 and d 10 to 24, respectively, with a common phase 3 diet fed until d 42. There was a tendency for an effect of CHO source (P = 0.058) on G:F during the treatment period, in which pigs fed the NC diet had the highest numeric G:F and pigs fed the 100% CHO-L diet had the least numeric G:F (Table 1). Overall, there was no observed effect of CHO source (P > 0.100) on ADG, ADFI, or fecal dry matter. In summary, feeding either of the novel CHO sources did not significantly affect growth performance or fecal dry matter during the nursery period compared with those pigs fed a traditional lactose source or a diet that did not contain any lactose. Based on the results herein, pigs fed diets containing either novel CHO product had equivalent performance to those on the PC treatment, but we were unable to detect incremental value as the PC treatment did not significantly differ from the NC treatment.
PSV-16 The Effects of Bodyweight-Basis Iron Injection to Newborn Piglets on Pre-And Post-Weaning Growth Performance and Hematological Parameters
Authors: Rank Ana N , Farrey Isaac W , Lebal Orianna N , Jang Young Dal
Published: October 2023

The objective of this study was to evaluate the effects of iron injection to newborn pigs based on birth weight on pre- and post-weaning growth performance and hematological variables. At d 1-3 of age (d 0 of experiment), a total of 24 piglets (initial body weight: 1.55 ± 0.11 kg) from 4 litters were allotted into 3 treatments within litter based on body weight and sex as follows: 1) Fe100: 100 mg iron at d 0 of experiment, 2) Fe200: 200 mg Fe at d 0 of experiment, and 3) FeBW: 125 mg Fe per kg body weight at d 0 of experiment. The iron-dextran was injected intramuscularly at the assigned levels based on treatments. The pigs were housed by litter in the suckling and nursery periods with a common corn-soybean meal-based diet during the entire nursery period. Body weight, hemoglobin concentrations and hematocrit were measured at d 0, 10, 17, 24 (weaning), 31 and 38 after the iron injection. There was no difference in pre- and post-weaning growth performance (10.91, 11.53, and 10.90 kg body weight at d 38 of experiment for the Fe100, Fe200, and FeBW treatments, respectively). Compared with the control treatment, the hemoglobin concentrations were greater in the Fe200 and FeBW treatments (P < 0.06) at d 17, 24, and 31 (12.43, 13.99, and 14.65 g/dL for the Fe100, Fe200, and FeBW treatments, respectively) of experiment with greater values in the FeBW treatment than the Fe200 treatment (P = 0.08; tendency) at d 31 of experiment. Hematocrit levels were greater in the Fe200 and FeBW treatments than the control treatment at d 10 (P = 0.09) and 31 of experiment (P < 0.05; 30.97, 35.57, and 37.07% for the Fe100, Fe200, and FeBW, respectively) and tended to be greater (P < 0.10) in the FeBW treatment than the control treatment at d 17 (31.71, 33.81, and 38.44% for the Fe100, Fe200, and FeBW, respectively) and 24 of experiment. However, there were no differences in hemoglobin concentrations and hematocrit at d 38 of experiment. In conclusion, there was no beneficial effect of iron injection based on body weight in pre- and post-weaning growth performance whereas it had a potential to slightly increase hemoglobin concentrations and hematocrit when the iron was injected based on body weight of newborn piglets.
PSVI-13 Nutritional Value of Corn Sourced from US, Brazil, and Argentina on Growth Performance, Energy Digestibility, and Amino Acid Digestibility in Pigs
Authors: Sullivan Caitlyn R , Jansen Jordan K , Hong Jinsu , Palencia Jorge Perez , Levesque Crystal L , Thaler Robert C
Published: October 2023

This study was conducted to identify the nutritional value of corn from US, Brazil (BRZ), and Argentina (ARG). A total of 96 nursery pigs [initial body weight (BW) = 8.79 kg] were used in the feeding trial for 25 days and fed one of three diets with sole corn source (3 treatments, 8 replicates, 4 pigs/pen) in a randomized complete block design. The BW and feed consumption of pigs were measured every week to calculate the average daily gain (ADG) and gain to feed ratio (G:F). Twelve barrows (average BW = 23.7 ± 2.6 kg) housed in metabolic crates were fed one of three experimental diets with corn as a sole energy source to determine the energy value of the corn. The pigs had 7 d of adaption, followed by 4 d of fecal and urine collection with a total collection method for two periods (3 treatments, 8 replicates, 1 pig/crate). Ten ileal-cannulated pigs (average BW = 20 kg) were fed one of four diets, such as corn starch-based diet with different corn sources, and a nitrogen-free diet supplemented with titanium dioxide, in a 4 x 4 Latin Square design (4 treatments, 10 replicates, 1 pig/crate) to determine the ileal digestibility of amino acids by using an indicator method. The ileal-cannulated pigs had 5 days of adaptation, followed by 2 days of ileal digesta collection. Pigs were fed the diets at three times of maintenance energy requirement according to NRC (2012) in two equal meals daily. Pigs fed the US corn diet showed greater (P < 0.05) ADG and G:F for d 0 to 7 than those of pigs fed ARG diet by 13% and 16%, respectively, and tended to have greater (P < 0.10) ADG and G:F for d 0 to 7 than those of pigs fed BRZ corn diet. Pigs fed the US corn diet had greater (P < 0.05) G:F for the overall period (d 0 to 25) than that of pigs fed the BRZ diet by 4% and had a tendency of greater (P < 0.10) G:F for the overall period than that of pigs fed the ARG diet. Dietary corn sources have no significant influence on the standardized ileal digestibility of amino acids and crude protein in pigs. The metabolizable energy values for US, ARG, and BRZ were 3,297 kcal/kg, 3,297 kcal/kg, and 3,240 kcal/kg, respectively. The net energy values for US, ARG, and BRZ were 2,644 kcal/kg, 2,636 kcal/kg, and 2,592 kcal/kg, respectively. However, dietary corn sources had no significant differences in metabolizable energy and net energy values. In conclusion, dietary US corn improved the growth performance of nursery pigs through the improved energy values in pigs compared with the Brazil and Argentina corns.
PSVI-20 Trends in Mycotoxin Contamination of the 2022 United States Corn Crop
Authors: Zheng Lan , Gott Paige N , Hofstetter-Schähs Ursula , Levy April W
Published: October 2023

Mycotoxins are harmful secondary fungal metabolites detrimental to animal health, even at reduced quantities. Focus on clinical signs such as decreased feed intake and vomiting overlook impacts of mycotoxicosis, including increased disease incidence and severity, immune modulation, inflammation, and gastrointestinal disorders. This study was conducted to compare mycotoxin contamination of initial samples of the 2022 corn crop with previous year trends. Samples were analyzed utilizing the liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for six major mycotoxin groups: aflatoxins (Afla), type A trichothecenes (A-Trich), type B trichothecenes (B-Trich), fumonisins (FUM), zearalenone (ZEN), and ochratoxin A (OTA). The 2022 crop year data were compared with the prior four harvest years using GLIMMIX procedure of SAS with harvest year as fixed effect and sample as the experimental unit. A limited number of samples is available thus far (n = 44) as harvest is still progressing, therefore the risk profile of this crop year is likely to change as the sample pool expands. Contamination levels in corn for the tested toxin groups have remained consistent over the survey period. To date, 97% of the samples evaluated contained at least one mycotoxin, compared with 90% that was observed in 2021. The percentage of Afla contaminated samples increased (P < 0.05) in 2022 corn compared with the 2020 and 2021 harvest years (42 vs. 5 and 7%). The percentage of FUM contaminated samples in 2022 corn is greater (P < 0.05) compared with 2020 and 2021 (88 vs. 64 and 64%). The preliminary results of the 2022 US corn crop survey indicate mycotoxin occurrence and contamination levels are similar or greater (Afla and FUM) to the prior crop year and will continue to challenge animal health and performance as these ingredients are fed out.
PSVI-12 Nutritional Value of Corn Sourced from U.S. and Ukraine on Growth Performance, Energy Digestibility, and Amino Acid Digestibility in Pigs
Authors: Sullivan Caitlyn R , Hong Jinsu , Levesque Crystal L , Thaler Robert C
Published: October 2023

This study was conducted to investigate the nutritional value of corn sourced from the US and Ukraine (UKR) in the swine diet. A total of 64 nursery pigs [initial body weight (BW) = 8.24 ± 0.73 kg] were used in the feeding trial for 28 days and fed one of two diets with sole corn source from US and Ukraine (2 treatments, 8 replicates, 4 pigs/pen) in a randomized complete block design. Pig BW and feed consumption were measured weekly basis to calculate the average daily gain and gain to feed ratio. Seven ileal-cannulated pigs (average BW = 20.3 ± 2.4 kg) were housed in metabolic crates for 3 periods, and the pigs were fed one of three experimental diets (3 treatments, 7 replicates, 1 pig/crate) such as corn starch-based with corn as a sole protein source to determine the ileal digestibility of amino acids by an indicator method. Each period had 5 days of adaptation, followed by 2 days of ileal digesta collection. Eight ileal-cannulated pigs (average BW = 20.0 ± 3.7 kg) were housed in metabolic crates, and the pigs were fed one of two diets with corn as a sole energy source. The pigs had 5 days of adaption, followed by 4 days of fecal and urine collection by using a total collection method for two periods (2 treatments, 8 replicates, 1 pig/crate). Pigs were fed the diets at three times of maintenance energy requirement according to NRC (2012) in two equal meals daily. In the feeding trial, dietary US or UKR corn did not affect the BW, average daily gain, average daily feed intake, and gain to feed ratio in nursery pigs. The standardized ileal digestibility (SID) of Arg for UKR corn was greater (P < 0.05) than US corn, and the SID of His, Phe, Val, Ala, Asp, Cys, and Glu for UKR corn tended to be greater (P < 0.10) than those for US corn. The analyzed gross energy values for US and UKR corns were 3,843 kcal/kg and 3,937 kcal/kg, respectively. The metabolizable energy for UKR corn was greater (P < 0.05) than US corn (3,406 vs. 3,229 kcal/kg). The net energy value for UKR tended to be greater (P < 0.10) than US corn (2,777 vs. 2714 kcal/kg). In conclusion, supplementation of US corn in the nursery diet had no difference in the growth performance of the pigs compared with the UKR corn, whereas the SID value of arginine and metabolizable energy value for US corn were less than those for UKR corn.
PSVI-7 Digestible Energy and Metabolizable Energy Concentrations of Corn, Oilseed Meals, and Fibrous Ingredients for Growing Pig
Authors: Son Jeonghyeon , Kim Beob G
Published: October 2023

The objective of the present work was to determine digestible energy (DE) and metabolizable energy (ME) concentrations of corn, oilseed meals, and fibrous ingredients fed to growing pig. Twelve barrows with an initial body weight of 34.3 kg (SD = 2.9) were individually housed in metabolism crates. A 12 × 8 incomplete Latin square design with 12 animals, 12 experimental diets, and 8 periods was employed. A basal diet contained 76.7% corn, 19.0% soybean meal (SBM), and 2% soybean oil as the sole source of energy. Ten experimental diets were prepared by replacing 30% of the basal diet with SBM, rapeseed meal, rice bran, corn gluten feed, corn distillers dried grain with solubles (DDGS), wheat bran, sugar beet pulp, copra meal, soybean hulls, or palm kernel expellers. Additionally, a corn diet contained 97.7% of corn as a sole energy source. Each period consisted of a 5-day adaptation period and a 5-day collection period, and the marker-to-marker procedure was used for the quantitative collection of feces and urine. The gross energy concentrations in the experimental diets were close to the calculated values based on the ingredient gross energy values. The DE and ME concentrations in corn (3,911 and 3,866 kcal/kg DM) and SBM (4,004 and 3,780 kcal/kg DM) were greatest (P < 0.05) among the 11 test ingredients followed by DDGS (3,531 and 3,336 kcal/kg DM), rice bran (3,242 and 3,172 kcal/kg DM), soybean hulls (3,081 and 3,103 kcal/kg DM), palm kernel expellers (3,112 and 3,058 kcal/kg DM). The DE and ME concentrations in corn gluten feed (2,624 and 2,460 kcal/kg DM) were less (P < 0.05) than those in all other test ingredients. In conclusion, corn and SBM had the greatest energy values among the corn, oilseed meals, and fibrous ingredients, whereas corn gluten feed had the lowest energy values.
PSVI-8 Effects of Dietary Supplementation of Pediococcus Pentosaceus Strains Isolated from White Kimchi in Weaned Piglet Challenged with Escherichia Coli and Salmonella Typhimurium
Authors: Song Dongcheol , Oh Hanjin , Kim Yongju , An Jaewoo , Chang Seyeon , Go Youngbin , Cho Hyunah , Park Sehyun , Jeon Kyungho , Cho Jinho
Published: October 2023

Escherichia coli and Salmonella typhimurium infections in pigs are major source associated with enteric disease such as post-weaning diarrhea. Lactic acid bacteria, one of the most essential probiotics, can colonize the digestive tract and increase nutritional digestion and maintain the stability of the intestinal flora. The aim of this study was to investigate the effects of Pediococcus pentosaceus in weaned piglets challenged with pathogen bacteria. In exp.1, 90 weaned piglets with initial body weights of 8.53 ± 0. 34 kg were assigned to 15 treatments for 2 weeks. The experiments were conducted two trials in a 2 × 5 factorial arrangement of treatments consisting of two levels of challenge (challenge and non-challenge) with E.coli and Salmonella typhimurium, respectively and five levels of probiotics (Control, Lactobacillus plantarum, Pediococcus pentosaceus SMFM2016-WK1, Pediococcus acidilactici K, Lactobacillus reuteri PF30). In exp.2 a total of 30 weaned pigs (initial body weight of 9.84 ± 0. 85kg) were used in 4 weeks experiment. Pigs were allocated to 5 groups in a randomized complete way with 2 pens per group and 3 pigs per pen. Supplementation of Lactobacillus plantarum and Pediococcus pentosaceus SMFM2016-WK1 improved (P < 0.05) growth performance, intestinal pathogen bacteria counts compared with control group in exp.1. Also, Lactobacillus plantarum and Pediococcus pentosaceus SMFM2016-WK1 improved (P < 0.05) fecal noxious odor, diarrhea incidence and digestibility of crude protein and gross energy compared with control group in exp.2. In conclusion, supplementation of 38W strains isolated from white kimchi can act as probiotics by inhibiting E.coli and Salmonella typhimurium.
PSIV-9 Salmate Dha+ Fish Oil Supplement Fed to Boars and Impact on Sperm Production and Spermatogenesis
Authors: Parrish John J , Gall Tom , Wilson Mark , Parsley Mike , Ballard Malcolm
Published: October 2023

Previous studies have found omega-3 fatty acid supplementation increased sperm production in boars, but the mechanism has not been demonstrated. The current study was undertaken to determine if the increase in sperm production was due to a reduction in germ cell apoptosis during spermatogenesis. Mature Duroc boars (438 to 686 days of age) were fed either a control diet of corn, soybean meal, 5% fiber, plus vitamins and minerals per NRC requirements or the control diet plus a proprietary blend of fish oil, Salmate DHA+ supplemented diet with 1.83 gm DHA (Docosahexaenoic Acid, c22:6 omega3) from 26 gm Salmate DHA+ (The Ballard Group, Inc. and Feedworks USA, LTD.) ∙animal-1∙day-1 added to the control diet. The control diet was fed to all boars (n = 10) for 62 days and then Salmate DHA+ was added to the diet of 5 of the boars while the other 5 continued the control diet for another 62 days. At the end of the experiment all boars were slaughtered, the testes collected, slices removed, fixed with Karnovsky’s fixative, post-fixed with 70% ethanol, embedded with acrylic resin, 2-micron sections taken, and sections stained with Toluidine Blue. During the control and Salmate DHA+ feeding, boars were on routine semen collection. The boar stud provided the date of collection, total sperm collected, and age of individual boars. To confirm the impact on sperm production, semen data was evaluated for all boars on the first 62 days of the control diet (before period) and then for control and Salmate DHA+ boars from day 22 to 62 after Salmate DHA+ feeding started (after period). A total of 107 ejaculates were evaluated in the study. Total sperm per ejaculate (X 109) of the control boars was 105.7 ± 7.3 vs. 110.9 ± 10.4 in the before vs. after period (P >0.05). The total sperm per ejaculate (X 109) in the Salmate DHA+ boarswas 103.4 ± 5 vs. 122.8 ± 7 in the before vs. after period (P < 0.05). To examine apoptosis via histology, the number of round spermatids (RS) and Sertoli cells (SC) were counted in 10 stage 1 seminiferous tubules for each boar with adjustments made for cell nucleoli or nuclei size and thickness of the histology section. The ratio of RS/SC or Sertoli Cell Index was then determined to estimate how many RS survived apoptosis in spermatogenesis. The Sertoli Cell index was 9.46 ± 0.84 vs. 15.03 ± 0.97, respectively, for Control and Salmate DHA+ boars (P < 0.01). The number of RS/SC increased 1.59-fold due to Salmate DHA+ treatment and may explain the increased sperm per ejaculate after Salmate DHA+treatment. The exact mechanism of how apoptosis was reduced however awaits further research. Work was supported by The Ballard Group, Inc and Feedworks USA, LTD.
PSV-10 Supplementing Amino Acid Complexed or Inorganic Trace Minerals to Grazing Cattle Following a Receiving Phase Dietary Trace Mineral Comparison
Authors: Cheek Robin A , Kegley Elizabeth B , Russel Jason R , Horton Lucas , Depenbusch Brandon , Reynolds Jana L , Shoulders Benjamin P , Midkiff Kirsten A , Powell Jeremy G
Published: October 2023

The objective was to investigate the effects of inorganic or amino acid-complexed sources of trace minerals (zinc and copper) on performance of beef heifers during a grazing period following a receiving trial. Crossbred beef heifer calves (n = 287, initial body weight = 231 ± 0.49 kg) were used in a 42-day receiving trial. Any cattle that failed to gain at least 0.45 kg/d, and(or) received 3 doses of antibiotic therapy during the receiving trial were removed from the study. Remaining cattle were kept within their respective pen and treatment and were randomly retained for the grazing phase. If there were not enough eligible calves/pen, calves on the same treatment that had been randomly removed from other pens were added to achieve equal pen counts. Cattle (n = 204; initial body weight = 262 ± 1.44 kg) grazed at 2 different locations in Arkansas; in Fayetteville, cattle (12 pens; 6 pens/treatment) grazed 6-acre stockpiled mixed grass pastures (n = 8 calves/pasture); in Batesville, cattle (12 pens; 6 pens/treatment) grazed 5-acre stockpiled novel-endophyte fescue pastures (n = 9 calves/pasture). Treatments consisted of supplemental zinc (540 mg/d) and copper (90 mg/d) from complexed (Availa, Zinpro Corp. Eden Prairie, MN) or inorganic sources (sulfates). Cattle grazed for at least 114 days until reaching a weight goal of 341 kg. Cattle had ad libitum access to water and were offered bermudagrass hay when forage was limited. Body weights were measured on 28-day intervals. A subset of calves (3/pen) were liver biopsied at the end of receiving and grazing phases to compare liver mineral concentrations. Following grazing, calves were removed from treatments and sent to a Kansas feedlot (n = 198) where they were commingled and fed in a single pen for 141 days. Morbidity and mortality data were recorded, and after slaughter, carcass data were collected. Statistical analyses were performed using MIXED and GLIMMIX procedures of SAS 9.4 with location as a random effect and pen within location specified as subject. There was no treatment (P = 0.14) or treatment × day interaction (P = 0.92) for body weights during the grazing phase. Overall average daily gain was not different between treatments (P = 0.94; 0.69, SE = 0.04). Treatment did not affect liver mineral concentrations (P ≥ 0.17). There were no differences in feedlot morbidity, mortality, or carcass characteristics (P ≥ 0.28). In conclusion, although complexed sources of trace minerals (zinc, copper, manganese, and cobalt) improved body weight gain and decreased morbidity treatments during the receiving phase, there were no significant differences from grazing to slaughter when supplementing amino acid complexed versus inorganic mineral sources of zinc and copper during the grazing period.
PSVI-5 Using Red Clover Extract to Mitigate Tall Fescue Toxicosis
Authors: Thomas Ivan R , Coffey Ken , Nieman Christine C , Grote Ally J , Alvey Sarah W
Published: October 2023

Tall fescue toxicosis is caused by ergot alkaloids produced by the fungus Neotyphodium coenophialum that resides within the majority of the tall fescue plants in the Southeastern and lower Midwestern US. Tall fescue (Festuca arundinacea) toxicosis is estimated to cost livestock producers over $1 billion dollars annually through reduced growth and reproductive performance. Improvements in performance by cattle grazing toxic tall fescue can have substantial economic advantages for livestock producers throughout the tall fescue region. Numerous methods to mitigate tall fescue toxicosis have been tried over the past 60+ years, but a practical solution to this problem has not been discovered to date. Forages used to dilute fescue include clovers which will not compete on much of the marginal sites that tall fescue predominates. As mentioned above, clovers have been used to dilute toxic fescue and have shown to be a good mitigation strategy if the clovers persist. Red clover extract (RCE) has shown promise in the mitigation of symptoms related to tall fescue toxicosis, but effective dosages have not yet been determined to date. Dorper ewe and wether lambs (n = 30, 34 ± 0.97 kg initial BW) were weighed and assigned randomly to one of five diets in a 2-period study. Diets consisted of a positive control of non-toxic tall fescue seed with no RCE (CONT), a negative control of toxic tall fescue seed with no RCE (E+0) and toxic tall fescue seed with RCE supplemented at 0.33 (E+33), 0.67 (E+67), and 1 (E+100) percent of the diet. Fescue seed was mixed with liquid molasses to ensure consumption, and seed, DDGS and RCE was offered at approximately 0800 and 1600 h, and bermudagrass hay was offered for ad libitum consumption. Lambs were housed in individual 1 × 1.5 m pens in an enclosed building where temperature was maintained at 27° C (80° F). The animal had an ad libitum access to water. Each period consisted of a 14-day adaptation period followed by 7 d of total fecal and urine collection. Diets were offered for ad libitum consumption during the first 12 days of each period and then held constant during the remaining 9 days. Dry matter (DM) and organic matter (OM) intake was greater (P < 0.05) from CONT than all other treatments and E+33 was greater (P < 0.05) than E+10 and E+67. Digestibility of DM and OM were greater (P < 0.05) from CONT than all other treatments. By contrast, the diets did not influence (P > 0.05) nitrogen balance, urinary nitrogen losses, fecal nitrogen losses and nitrogen retained. Overall, results demonstrated that RCE inclusion into the diet of toxic tall fescue did not significantly change growth and performance parameters in sheep.
PSIV-3 Biofumigation to Reduce Swine Parasite Contamination on Organic Pastures
Authors: Li Yuzhi , Major Sara , Carr Rick , Hernandex Alexander
Published: October 2023

Parasite infection is a common problem in organic pig production. Pastures grazed by infected pigs can become sources of parasite transmission because parasite eggs can survive in soil for several years. Biofumigation is a method used by farmers to reduce plant pathogens and nematodes in soil by incorporating plant residues into the soil that rapidly release toxic isothiocyanates. This study explored whether grazing crops with biofumigation properties affects parasite fecal egg counts of pigs managed organically, and subsequent parasite eggs in organic pasture soil after biofumigation. A study was conducted using 66 pigs (BW = 72.6 kg ± 21.2 SD) that were raised organically in two blocks. Within each block, pigs were housed in four pens (8 to 9 pigs/pen) and provided feed ad libitum in a hoop barn. Each pen had access to a pasture (0.5 acres). Each pasture was subjected to one of four management treatments: control pastures (Ladino clover) or biofumigation pastures (Rapeseed), each with or without incorporating plant residues. Each pasture was split into four paddocks (9.1m × 10.7m) and pigs were given access to each paddock for one week. Fecal samples (n = 198) were collected from each pig before, two and four weeks after grazing. Triple soil samples (n = 480) were collected from each paddock before grazing, immediately after pigs were removed from pasture paddocks, and weekly thereafter for three weeks. Fecal and soil samples were analyzed for Ascaris suum, Trichuris suis, and Oesphagostomum spp. eggs. Data were analyzed using a mixed model with pen or paddock as the experimental unit. Fecal egg counts of the three parasite species did not differ between pigs that grazed on pastures with different management treatments. All pigs had drecreased Ascaris suum (P < 0.01) and Trichuris suis (P < 0.01) fecal egg counts after four weeks of grazing compared with before grazing. Both clover and rapeseed pastures without incorporating plant residue had decreased Ascaris suum egg counts in the soil compared with incorporating plant residue (both P < 0.05). Rapeseed pastures without incorporating plant residue had reduced (P < 0.05) Trichuris suis egg counts in the soil compared with clover pastures but did not differ from rapeseed pasture with incorporated plant residue. Across pasture management treatments, Ascaris suum and Trichuris suis eggs in the soil were reduced (P < 0.05) two and three weeks after pigs were removed from pastures compared with before, immediately after, and one week after pigs were removed. These results suggest that grazing pastures crops with biofumigation properties did not affect fecal egg counts of pigs. While the number of parasite eggs decreased overtime in pasture soil, biofumigation may not be an effective way to reduce swine parasite eggs in organic pastures.
PSIV-5 The Utilization of Alltech’s Seed, Feed, Weed Piglet Program as an Alternative to Zinc Oxide on the Growth, Feed Efficiency and Mortality of Newly Weaned Pigs
Authors: Rooney Hazel B , Roquet Josep , Santos Manuel , Taylor-Pickard Jules A
Published: October 2023

Achieving a healthy gut with a suitable microbial population in place will maximize the health and lifetime performance of piglets and, concurrently, profitability. Natural nutritional strategies that promote gut health and development in young pigs without zinc oxide (ZnO) are gaining considerable interest. Alltech’s Seed, Feed, Weed (SFW) program is designed to promote favorable microbial communities in the gut, optimize gut structures, and support the immune system; all of which will positively impact the growth and health of young piglets. The objective of this study was to investigate the effect of the SFW piglet program as an alternative to ZnO supplementation on the growth, feed efficiency and mortality of newly weaned pigs. Newly weaned pigs were allocated to one of two dietary treatments in 3 experiments. All experiments were carried out on commercial pig units in Spain. Dietary treatments were a basal pre-starter and starter diet containing 3,000 ppm ZnO (CONTROL), and a SFW piglet pre-starter and starter pack containing 0 ppm ZnO (SFW). In Exp. 1, pigs (n = 431) were weaned at 28 days old and fed the experimental pre-starter diets for 9 days. In Exp. 2, pigs (n = 295) were weaned at 28 days old and fed the experimental pre-starter and starter diets for 35 days. In Exp. 3, pigs (n = 311) were weaned at 28 days old and fed the experimental pre-starter and starter diets for 21 days. Pig performance was recorded. In experiment 1, SFW-fed pigs had similar average daily gain (ADG; 142 g/d vs 148 g/d) and average daily feed intake (ADFI; 149 g/d vs. 167 g/d) to CONTROL fed pigs. Feed efficiency (FE) was 1.05 and 1.13 for SFW and CONTROL piglets, respectively, resulting in SFW fed piglets being heavier at the end of the treatment period (7.65 kg vs. 7.45 kg). In experiment 2, overall, ADG (308 g/d vs. 316 g/d) and ADFI (423 g/d vs. 444 g/d) were similar between SFW and CONTROL fed piglets, respectively. Th SFW piglets had greater FE than CONTROL pigs (1.37 vs. 1.41). In experiment 3, there was no effect of treatment on ADG (356 g/d vs. 355 g/d) and ADFI (410 g/d vs. 420 g/d) or piglet body weight at the end of the study (12.8 kg vs. 12.7 kg). Feed efficiency was improved (1.21 vs. 1.24) and piglet mortality was reduced (3.4 % vs. 6.9 %) in SFW fed piglets compared with CONTROL fed piglets. In conclusion, the SFW program may prove to be a suitable alternative to ZnO by positively influencing the gut health of piglets and ensuring that piglet performance and health is maintained in the absence of ZnO.
Narrative verbal forms in North-Eastern Neo-Aramaic dialects
Authors: Khan Geoffrey
Published: October 2023

I examine various types of verbal forms used in narratives of the North-Eastern Neo-Aramaic (NENA) dialects. They have in common the feature of dependence on the preceding discourse. These dependent narrative forms can be classified into ‘underspecified forms’ and ‘subordinate forms’. The underspecified forms include habitual forms and perfect forms, both of which lack the full specification of an event in their prototypical grammatical meaning. The specification of the event depends on the narrative context. The subordinate narrative forms have developed by a process of insubordination whereby their use has been extended from syntactically subordinate clauses to main clauses that are semantically dependent on the preceding discourse. One factor that may have motivated this insubordination process in the case of the narrative subjunctive qaṭəl form is the conservative continuity of the morphological shape of the narrative qāṭēl form, which was a feature of earlier Aramaic narrative style.
A Note on the Safaitic Calendar
Authors: Al-Jallad Ahmad
Published: October 2023

This short contribution presents three new Safaitic texts that shed further light on the division of the year among the pre-Islamic nomads of the Syro-Arabian Ḥarrah. The inscriptions demonstrate that the zodiac sign names could be used as the names of months and open a new avenue of discussion regarding the meanings of the terms rʾy and ksʾ.
Responsive and Adaptive Designs in Repeated Cross-National Surveys: A Simulation Study
Authors: Einarsson Hafsteinn , Cernat Alexandru , Shlomo Natalie
Published: October 2023

Cross-national surveys run the risk of differential survey errors, where data collected vary in quality from country to country. Responsive and adaptive survey designs (RASDs) have been proposed as a way to reduce survey errors, by leveraging auxiliary variables to inform fieldwork efforts, but have rarely been considered in the context of cross-national surveys. Using data from the European Social Survey, we simulate fieldwork in a repeated cross-national survey using RASD where fieldwork efforts are ended early for selected units in the final stage of data collection. Demographic variables, paradata (interviewer observations), and contact data are used to inform fieldwork efforts. Eight combinations of response propensity models and selection mechanisms are evaluated in terms of sample composition (as measured by the coefficient of variation of response propensities), response rates, number of contact attempts saved, and effects on estimates of target variables in the survey. We find that sample balance can be improved in many country-round combinations. Response rates can be increased marginally and targeting high propensity respondents could lead to significant cost savings associated with making fewer contact attempts. Estimates of target variables are not changed by the case prioritizations used in the simulations, indicating that they do not impact nonresponse bias. We conclude that RASDs should be considered in cross-national surveys, but that more work is needed to identify suitable covariates to inform fieldwork efforts.
Increased DNA methylation of the splicing regulator SR45 suppresses seed abortion in litchi
Authors: Xie Hanhan , Yin Wenya , Zheng Yedan , Zhang Yanshan , Qin Hongming , Huang Zhiqiang , Zhao Minglei , Li Jianguo
Published: October 2023

The gene regulatory networks that govern seed development are complex, yet very little is known about the genes and processes that are controlled by DNA methylation. Here, we performed single-base resolution DNA methylome analysis and found that CHH methylation increased significantly throughout seed development in litchi. Based on the association analysis of differentially methylated regions and weighted gene co-expression network analysis (WGCNA), 46 genes were identified as essential DNA methylation-regulated candidate genes involved in litchi seed development, including LcSR45, a homolog of the serine/arginine-rich (SR) splicing regulator SR45. LcSR45 is predominately expressed in the funicle, embryo, and seed integument, and displayed increased CHH methylation in the promoter during seed development. Notably, silencing of LcSR45 in a seed-aborted litchi cultivar significantly improved normal seed development, whereas the ectopic expression of LcSR45 in Arabidopsis caused seed abortion. Furthermore, LcSR45-dependent alternative splicing events were found to regulate genes involved in seed development. Together, our findings demonstrate that LcSR45 is hypermethylated, and plays a detrimental role in litchi seed development, indicating a global increase in DNA methylation at this stage.
Leadership Styles Experienced During Military Service Predict Later Anhedonic Depressive Symptoms and Self-Efficacy in Veterans With Alcohol Use Disorder
Authors: Durazzo Timothy C , Humphreys Keith , LaRocca Michael A
Published: October 2023

Introduction

Lifetime and past-year alcohol use disorder (AUD) prevalence is significantly higher in US Armed Services Veterans than in non-veterans across adulthood. This study examined the associations of perceived transformational leadership styles (TLS) experienced during military service and anhedonic depression and self-efficacy related to confidence to abstain or reduce alcohol consumption in Veterans seeking treatment for AUD. The ramifications of perceived leadership styles on multiple aspects of follower psychiatric functioning, including depressive and PTSD symptomatology, during and after military service, may be substantial and enduring. Higher anhedonic depression and lower abstinence self-efficacy are related to increased risk of relapse after treatment. We predicted Veterans, in treatment for AUD, who reported higher perceived levels of transformational leadership during military service, demonstrate lower anhedonic depressive symptoms and higher alcohol abstinence self-efficacy.

Materials and Methods

Veterans with AUD (n = 60; 50 ± 14 years of age) were recruited from residential treatment at the VA Palo Alto Health Care System. All procedures were approved by the VA Palo Alto Health Care System and Stanford University institutional review boards. A series of mediation analyses were completed with The Multifactor Leadership Questionnaire measures of TLS (average across leadership measures [transformational leadership average; TLS average]) as predictor and the Alcohol Abstinence Self-Efficacy Scale, Mood and Anxiety Symptom Questionnaire, anhedonic depression subscale, as dependent measures. PTSD Checklist for DSM-5 score was tested as a mediator variable.

Results

Higher reported perceived TLS average during military service was significantly related to lower anhedonic depressive symptoms. Higher TLS average was related to higher self-efficacy to resist alcohol use in contexts involving experience of physical issues and withdrawal/cravings and urges. These relationships were not mediated by PTSD symptomatology or duration of military service, age, education, time since military service, military branch, combat exposure, or current psychiatric diagnosis.

Conclusions

The significant associations of perceived TLS during military service with anhedonic depression and alcohol use self-efficacy are clinically relevant because these measures are associated with relapse risk after AUD treatment. Further study of the implications of perceived TLS during military service for AUD and other substance use disorder treatment outcome is warranted in Veterans.
Application Times, Placement Accuracy, and User Ratings of Commercially Available Manual and Battery-Powered Intraosseous Catheters in a High Bone Density Cadaveric Swine Model
Authors: Kay Victoria C , Gehrz Joseph A , Grady Derek W , Emerling Alec D , McGowan Andrew , Reilly Erin R , Bebarta Vikhyat S , Nassiri Joshua , Vinals Jorge , Schrader Andrew , Zarow Gregory J , Auten Jonathan D
Published: October 2023

Introduction

Intraosseous (IO) infusion, the pressurized injection of fluids into bone through a catheter, is a life-preserving resuscitative technique for treating trauma patients with severe hemorrhage. However, little is known regarding the application times, placement accuracy, and end-user ratings of battery-powered and manual IO access devices. This study was specifically designed to fill these knowledge gaps on six FDA-approved IO access devices.

Materials and Methods

Three experienced U.S. Navy Emergency Medicine residents each placed commercially available 15-gauge IO catheters in cadaveric swine (Sus scrofa) proximal humeri and sternums in a randomized prospective experimental design. Devices included the battery-powered EZ-IO Rapid Infuser and the manual Jamshidi IO, PerSys NIO, SAM Manual IO, Tactical Advanced Lifesaving IO Needle (TALON), and PYNG First Access for Shock and Trauma 1 (30 trials per device, 10 per user, 210 total trials). Application times, placement accuracy in medullary (zone 1) and trabecular (zone 2) bone while avoiding cortical (zone 3) bone, and eight subjective user ratings were analyzed using ANOVA and nonparametric statistics at P < .05.

Results

The EZ-IO demonstrated the fastest application times, high rates in avoiding zone 3, and the highest user ratings (P < .0001). The TALON conferred intermediate placement times, highest rates of avoiding zone 3, and second-highest user ratings. The SAM Manual IO and Jamshidi performed poorly, with mixed results for the PerSys NIO and PYNG First Access for Shock and Trauma 1.

Conclusions

The battery-powered EZ-IO performed best and remains the IO access device of choice. The present findings suggest that the TALON should be considered as a manual backup to the EZ-IO.
Changing Tunes: Narratives of Nation in South African Music-Themed Postage Stamps (1961–2015)
Authors: Gerber Melissa
Published: October 2023

Postage stamps have in recent years been positioned as token purveyors of nation, with countries disseminating carefully curated ideas of national identity to their own citizens and international audiences. This article complements existing research by using South African music-themed postage stamps to read the changing narratives of the nation, or ‘music nationalism’ in philately. The article analyses music-themed postage stamps issued in South Africa between 1961 and 2015. It confirms that the representation of music nationalism in apartheid philately aligned with Afrikaner nationalist politics, with clear divides between ‘White’ and ‘Black’ genres. Post-apartheid music-themed stamps draw attention to forgotten music histories previously overshadowed by apartheid ideology while still engaging with musical threads of the apartheid past. Most significantly, the article illuminates the other forces at play behind what many accept to be a process solely steered and controlled by the state, revealing much about South Africa’s democracy.
Local models of two-temperature accretion disc coronae – I. Structure, outflows, and energetics
Authors: Bambic Christopher J , Quataert Eliot , Kunz Matthew W
Published: October 2023

We use local stratified shearing-box simulations to elucidate the impact of two-temperature thermodynamics on the thermal structure of coronae in radiatively efficient accretion flows. Rather than treating the coronal plasma as an isothermal fluid, we use a simple, parametrized cooling function that models the collisional transfer of energy from the ions to the rapidly cooling leptons. Two-temperature models naturally form temperature inversions, with a hot, magnetically dominated corona surrounding a cold disc. Simulations with net vertical flux (NF) magnetic fields launch powerful magnetocentrifugal winds that would enhance accretion in a global system. The outflow rates are much better converged with increasing box height than analogous isothermal simulations, suggesting that the winds into two-temperature coronae may be sufficiently strong to evaporate a thin disc and form a radiatively inefficient accretion flow under some conditions. We find evidence for multiphase structure in the corona, with broad density and temperature distributions, and we propose criteria for the formation of a multiphase corona. The fraction of cooling in the surface layers of the disc is substantially larger for NF fields compared to zero net-flux configurations, with moderate NF simulations radiating ≳30 per cent of the flow’s total luminosity above two mid-plane scale heights. Our work shows that NF fields may efficiently power the coronae of luminous Seyfert galaxies and quasars, providing compelling motivation for future studies of the heating mechanisms available to NF fields and the interplay of radiation with two-temperature thermodynamics.