bluuebunny/crossref_metadata_2025 huggingface dataset:
Data from MACdb: A Curated Knowledgebase for Metabolic Associations across Human Cancers
Authors: Sun Yanling , Zheng Xinchang , Wang Guoliang , Wang Yibo , Chen Xiaoning , Sun Jiani , Xiong Zhuang , Zhang Sisi , Wang Tianyi , Fan Zhuojing , Bu Congfan , Bao Yiming , Zhao Wenming
Published: April 2023

Abstract
Cancer is one of the leading causes of human death. As metabolomics techniques become more and more widely used in cancer research, metabolites are increasingly recognized as crucial factors in both cancer diagnosis and treatment. In this study, we developed MACdb (https://ngdc.cncb.ac.cn/macdb), a curated knowledgebase to recruit the metabolic associations between metabolites and cancers. Unlike conventional data-driven resources, MACdb integrates cancer-metabolic knowledge from extensive publications, providing high quality metabolite associations and tools to support multiple research purposes. In the current implementation, MACdb has integrated 40,710 cancer-metabolite associations, covering 267 traits from 17 categories of cancers with high incidence or mortality, based entirely on manual curation from 1,127 studies reported in 462 publications (screened from 5,153 research papers). MACdb offers intuitive browsing functions to explore associations at multi-dimensions (metabolite, trait, study, and publication), and constructs knowledge graph to provide overall landscape among cancer, trait, and metabolite. Furthermore, NameToCid (map metabolite name to PubChem Cid) and Enrichment tools are developed to help users enrich the association of metabolites with various cancer types and traits.
Implication:
MACdb paves an informative and practical way to evaluate cancer-metabolite associations and has a great potential to help researchers identify key predictive metabolic markers in cancers.
Data from Tumor-Derived CCL5 Recruits Cancer-Associated Fibroblasts and Promotes Tumor Cell Proliferation in Esophageal Squamous Cell Carcinoma
Authors: Dunbar Karen J. , Karakasheva Tatiana A. , Tang Qiaosi , Efe Gizem , Lin Eric W. , Harris Michael , Sahu Varun , Sachdeva Uma M. , Hu Jianhua , Klein-Szanto Andres J. , Henick Brian , Diehl J. Alan , Nakagawa Hiroshi , Rustgi Anil K.
Published: April 2023

Abstract
Cancer-associated fibroblasts (CAF) can promote tumor growth, metastasis, and therapeutic resistance in esophageal squamous cell carcinoma (ESCC), but the mechanisms of action remain elusive. Our objective was to identify secreted factor(s) that mediate the communication between CAFs and ESCC tumor cells with the aim of identifying potential druggable targets. Through unbiased cytokine arrays, we have identified CC motif chemokine ligand 5 (CCL5) as a secreted factor that is increased upon co-culture of ESCC cells and CAFs, which we replicated in esophageal adenocarcinoma (EAC) with CAFs. Loss of tumor-cell-derived CCL5 reduces ESCC cell proliferation in vitro and in vivo and we propose this is mediated, in part, by a reduction in ERK1/2 signaling. Loss of tumor-derived CCL5 reduces the percentage of CAFs recruited to xenograft tumors in vivo. CCL5 is a ligand for the CC motif receptor 5 (CCR5), for which a clinically approved inhibitor exists, namely Maraviroc. Maraviroc treatment reduced tumor volume, CAF recruitment, and ERK1/2 signaling in vivo, thus, mimicking the effects observed with genetic loss of CCL5. High CCL5 or CCR5 expression is associated with worse prognosis in low-grade esophageal carcinomas.
Implications:
These data highlight the role of CCL5 in tumorigenesis and the therapeutic potential of targeting the CCL5–CCR5 axis in ESCC.
Supplementary Figure S5 from Mitochondrial Metabolism Drives Low-density Lipoprotein-induced Breast Cancer Cell Migration
Authors: Nóbrega-Pereira Sandrina , Santos Francisco , Oliveira Santos Miguel , Serafim Teresa L. , Lopes Ana Patrícia , Coutinho Diogo , Carvalho Filipa S. , Domingues Rosário M. , Domingues Pedro , Bernardes de Jesus Bruno , Morais Vanessa A. , Dias Sérgio
Published: April 2023

LDL-induced migratory behavior of TNBC cells relies on fatty acid transport into the mitochondria. Related to Fig. 5
Supplementary Figure S4 from Mitochondrial Metabolism Drives Low-density Lipoprotein-induced Breast Cancer Cell Migration
Authors: Nóbrega-Pereira Sandrina , Santos Francisco , Oliveira Santos Miguel , Serafim Teresa L. , Lopes Ana Patrícia , Coutinho Diogo , Carvalho Filipa S. , Domingues Rosário M. , Domingues Pedro , Bernardes de Jesus Bruno , Morais Vanessa A. , Dias Sérgio
Published: April 2023

Lipid exposure induces metabolic and bioenergetic dependencies in breast cancer cells. Related to Fig. 4
Supplementary Table 2 from Dissecting the origin of heterogeneity in uterine and ovarian carcinosarcomas
Authors: Sertier Anne-Sophie , Ferrari Anthony , Pommier Roxane M. , Treilleux Isabelle , Boyault Sandrine , Devouassoux-Shisheboran Mojgan , Kielbassa Janice , Thomas Emilie , Tonon Laurie , Le Texier Vincent , Charreton Amandine , Morel Anne-Pierre , Floquet Anne , Joly Florence , Berton-Rigaud Dominique , Ferron Gwenaël , Arnould Laurent , Croce Sabrina , Bataillon Guillaume , Saintigny Pierre , Mery-Lamarche Eliane , Sagan Christine , Senaratne Aruni P. , Gut Ivo G. , Calvo Fabien , Viari Alain , Ouzounova Maria , Ray-Coquard Isabelle , Puisieux Alain
Published: April 2023

List of differentially expressed genes (paired analysis of 5 selected tumors).
Supplementary Table 1 from Dissecting the origin of heterogeneity in uterine and ovarian carcinosarcomas
Authors: Sertier Anne-Sophie , Ferrari Anthony , Pommier Roxane M. , Treilleux Isabelle , Boyault Sandrine , Devouassoux-Shisheboran Mojgan , Kielbassa Janice , Thomas Emilie , Tonon Laurie , Le Texier Vincent , Charreton Amandine , Morel Anne-Pierre , Floquet Anne , Joly Florence , Berton-Rigaud Dominique , Ferron Gwenaël , Arnould Laurent , Croce Sabrina , Bataillon Guillaume , Saintigny Pierre , Mery-Lamarche Eliane , Sagan Christine , Senaratne Aruni P. , Gut Ivo G. , Calvo Fabien , Viari Alain , Ouzounova Maria , Ray-Coquard Isabelle , Puisieux Alain
Published: April 2023

Curated list of chromatin remodeling genes.
Figure S9 from Dissecting the origin of heterogeneity in uterine and ovarian carcinosarcomas
Authors: Sertier Anne-Sophie , Ferrari Anthony , Pommier Roxane M. , Treilleux Isabelle , Boyault Sandrine , Devouassoux-Shisheboran Mojgan , Kielbassa Janice , Thomas Emilie , Tonon Laurie , Le Texier Vincent , Charreton Amandine , Morel Anne-Pierre , Floquet Anne , Joly Florence , Berton-Rigaud Dominique , Ferron Gwenaël , Arnould Laurent , Croce Sabrina , Bataillon Guillaume , Saintigny Pierre , Mery-Lamarche Eliane , Sagan Christine , Senaratne Aruni P. , Gut Ivo G. , Calvo Fabien , Viari Alain , Ouzounova Maria , Ray-Coquard Isabelle , Puisieux Alain
Published: April 2023

Clustering of somatic copy number alterations profiles.
Figure S6 from Dissecting the origin of heterogeneity in uterine and ovarian carcinosarcomas
Authors: Sertier Anne-Sophie , Ferrari Anthony , Pommier Roxane M. , Treilleux Isabelle , Boyault Sandrine , Devouassoux-Shisheboran Mojgan , Kielbassa Janice , Thomas Emilie , Tonon Laurie , Le Texier Vincent , Charreton Amandine , Morel Anne-Pierre , Floquet Anne , Joly Florence , Berton-Rigaud Dominique , Ferron Gwenaël , Arnould Laurent , Croce Sabrina , Bataillon Guillaume , Saintigny Pierre , Mery-Lamarche Eliane , Sagan Christine , Senaratne Aruni P. , Gut Ivo G. , Calvo Fabien , Viari Alain , Ouzounova Maria , Ray-Coquard Isabelle , Puisieux Alain
Published: April 2023

Alteration of genes of TP53, PI3K and cell cycle pathways in uterine and ovarian CS.
Figure S3 from Dissecting the origin of heterogeneity in uterine and ovarian carcinosarcomas
Authors: Sertier Anne-Sophie , Ferrari Anthony , Pommier Roxane M. , Treilleux Isabelle , Boyault Sandrine , Devouassoux-Shisheboran Mojgan , Kielbassa Janice , Thomas Emilie , Tonon Laurie , Le Texier Vincent , Charreton Amandine , Morel Anne-Pierre , Floquet Anne , Joly Florence , Berton-Rigaud Dominique , Ferron Gwenaël , Arnould Laurent , Croce Sabrina , Bataillon Guillaume , Saintigny Pierre , Mery-Lamarche Eliane , Sagan Christine , Senaratne Aruni P. , Gut Ivo G. , Calvo Fabien , Viari Alain , Ouzounova Maria , Ray-Coquard Isabelle , Puisieux Alain
Published: April 2023

Clustering of CS mutational signatures with COSMIC signatures.
Figure S2 from Dissecting the origin of heterogeneity in uterine and ovarian carcinosarcomas
Authors: Sertier Anne-Sophie , Ferrari Anthony , Pommier Roxane M. , Treilleux Isabelle , Boyault Sandrine , Devouassoux-Shisheboran Mojgan , Kielbassa Janice , Thomas Emilie , Tonon Laurie , Le Texier Vincent , Charreton Amandine , Morel Anne-Pierre , Floquet Anne , Joly Florence , Berton-Rigaud Dominique , Ferron Gwenaël , Arnould Laurent , Croce Sabrina , Bataillon Guillaume , Saintigny Pierre , Mery-Lamarche Eliane , Sagan Christine , Senaratne Aruni P. , Gut Ivo G. , Calvo Fabien , Viari Alain , Ouzounova Maria , Ray-Coquard Isabelle , Puisieux Alain
Published: April 2023

Heterogeneity of genomic rearrangements in carcinosarcoma.
Figure S13 from Dissecting the origin of heterogeneity in uterine and ovarian carcinosarcomas
Authors: Sertier Anne-Sophie , Ferrari Anthony , Pommier Roxane M. , Treilleux Isabelle , Boyault Sandrine , Devouassoux-Shisheboran Mojgan , Kielbassa Janice , Thomas Emilie , Tonon Laurie , Le Texier Vincent , Charreton Amandine , Morel Anne-Pierre , Floquet Anne , Joly Florence , Berton-Rigaud Dominique , Ferron Gwenaël , Arnould Laurent , Croce Sabrina , Bataillon Guillaume , Saintigny Pierre , Mery-Lamarche Eliane , Sagan Christine , Senaratne Aruni P. , Gut Ivo G. , Calvo Fabien , Viari Alain , Ouzounova Maria , Ray-Coquard Isabelle , Puisieux Alain
Published: April 2023

Complete clonal evolution of P01 tumor.
Figure S12 from Dissecting the origin of heterogeneity in uterine and ovarian carcinosarcomas
Authors: Sertier Anne-Sophie , Ferrari Anthony , Pommier Roxane M. , Treilleux Isabelle , Boyault Sandrine , Devouassoux-Shisheboran Mojgan , Kielbassa Janice , Thomas Emilie , Tonon Laurie , Le Texier Vincent , Charreton Amandine , Morel Anne-Pierre , Floquet Anne , Joly Florence , Berton-Rigaud Dominique , Ferron Gwenaël , Arnould Laurent , Croce Sabrina , Bataillon Guillaume , Saintigny Pierre , Mery-Lamarche Eliane , Sagan Christine , Senaratne Aruni P. , Gut Ivo G. , Calvo Fabien , Viari Alain , Ouzounova Maria , Ray-Coquard Isabelle , Puisieux Alain
Published: April 2023

Complete clonal evolution of P02 tumor.
Figure S11 from Dissecting the origin of heterogeneity in uterine and ovarian carcinosarcomas
Authors: Sertier Anne-Sophie , Ferrari Anthony , Pommier Roxane M. , Treilleux Isabelle , Boyault Sandrine , Devouassoux-Shisheboran Mojgan , Kielbassa Janice , Thomas Emilie , Tonon Laurie , Le Texier Vincent , Charreton Amandine , Morel Anne-Pierre , Floquet Anne , Joly Florence , Berton-Rigaud Dominique , Ferron Gwenaël , Arnould Laurent , Croce Sabrina , Bataillon Guillaume , Saintigny Pierre , Mery-Lamarche Eliane , Sagan Christine , Senaratne Aruni P. , Gut Ivo G. , Calvo Fabien , Viari Alain , Ouzounova Maria , Ray-Coquard Isabelle , Puisieux Alain
Published: April 2023

Differential methylation analysis (C vs S).
Figure S1 from Dissecting the origin of heterogeneity in uterine and ovarian carcinosarcomas
Authors: Sertier Anne-Sophie , Ferrari Anthony , Pommier Roxane M. , Treilleux Isabelle , Boyault Sandrine , Devouassoux-Shisheboran Mojgan , Kielbassa Janice , Thomas Emilie , Tonon Laurie , Le Texier Vincent , Charreton Amandine , Morel Anne-Pierre , Floquet Anne , Joly Florence , Berton-Rigaud Dominique , Ferron Gwenaël , Arnould Laurent , Croce Sabrina , Bataillon Guillaume , Saintigny Pierre , Mery-Lamarche Eliane , Sagan Christine , Senaratne Aruni P. , Gut Ivo G. , Calvo Fabien , Viari Alain , Ouzounova Maria , Ray-Coquard Isabelle , Puisieux Alain
Published: April 2023

Collection and selection of tumor samples.
Data from Mitochondrial Metabolism Drives Low-density Lipoprotein-induced Breast Cancer Cell Migration
Authors: Nóbrega-Pereira Sandrina , Santos Francisco , Oliveira Santos Miguel , Serafim Teresa L. , Lopes Ana Patrícia , Coutinho Diogo , Carvalho Filipa S. , Domingues Rosário M. , Domingues Pedro , Bernardes de Jesus Bruno , Morais Vanessa A. , Dias Sérgio
Published: April 2023

Most cancer-related deaths are due to metastases. Systemic factors, such as lipid-enriched environments [as low-density lipoprotein (LDL)-cholesterol], favor breast cancer, including triple-negative breast cancer (TNBC) metastasis formation. Mitochondria metabolism impacts TNBC invasive behavior but its involvement in a lipid-enriched setting is undisclosed. Here we show that LDL increases lipid droplets, induces CD36 and augments TNBC cells migration and invasion in vivo and in vitro. LDL induces higher mitochondrial mass and network spread in migrating cells, in an actin remodeling-dependent manner, and transcriptomic and energetic analyses revealed that LDL renders TNBC cells dependent on fatty acids (FA) usage for mitochondrial respiration. Indeed, engagement on FA transport into the mitochondria is required for LDL-induced migration and mitochondrial remodeling. Mechanistically, LDL treatment leads to mitochondrial long-chain fatty acid accumulation and increased reactive oxygen species (ROS) production. Importantly, CD36 or ROS blockade abolished LDL-induced cell migration and mitochondria metabolic adaptations. Our data suggest that LDL induces TNBC cells migration by reprogramming mitochondrial metabolism, revealing a new vulnerability in metastatic breast cancer.
Significance:
LDL induces breast cancer cell migration that relies on CD36 for mitochondrial metabolism and network remodeling, providing an antimetastatic metabolic strategy.
Terrestrial vertebrate and butterfly diversity of Garbhanga Landscape, Assam, India
Authors: Mahananda Pranjal , Jelil Shah Nawaz , Bohra Sanath Chandra , Mahanta Nilutpal , Saikia Rohini Ballave , Purkayastha Jayaditya
Published: April 2023

A study was conducted on the current status of terrestrial vertebrates and butterflies in Garbhanga Reserve Forest. Data were compiled from field surveys and secondary sources like existing literature, and citizen science databases.  We found that Garbhanga is represented by 254 species of butterflies (6 families), 29 species of amphibians (7 families), 64 species of reptiles (12 families), 307 species of birds (68 families) and 31 species of mammals (19 families). In Garbhanga, amongst the studied group, two species are ‘Critically Endangered’ (CR), six species are ‘Endangered’ (EN), 14 species are ‘Vulnerable’ (VU), eight species are ‘Near Threatened’ (NT), eight species are ‘Data Deficient’ (DD), 411 species are ‘Least Concern’ (LC), and 236 species are not assessed (denoted by blank space). This shows a huge gap in research and status evaluation of understudied species. This in turn also provides further research opportunities on the lesser known species.
Workplace Discrimination and Risk of Hypertension: Findings From a Prospective Cohort Study in the United States
Authors: Li Jian , Matthews Timothy A. , Clausen Thomas , Rugulies Reiner
Published: April 2023

Background

Mounting evidence has demonstrated a role of psychosocial stressors such as discrimination in hypertension and cardiovascular diseases. The objective of this study was to provide the first instance of research evidence examining prospective associations of workplace discrimination with onset of hypertension.

Methods and Results

Data were from MIDUS (Midlife in the United States), a prospective cohort study of adults in the United States. Baseline data were collected in 2004 to 2006, with an average 8‐year follow‐up period. Workers with self‐reported hypertension at baseline were excluded, yielding a sample size of 1246 participants for the main analysis. Workplace discrimination was assessed using a validated 6‐item instrument. During follow‐up with 9923.17 person‐years, 319 workers reported onset of hypertension, and incidence rates of hypertension were 25.90, 30.84, and 39.33 per 1000 person‐years among participants with low, intermediate, and high levels of workplace discrimination, respectively. Cox proportional hazards regression analyses demonstrated that workers who experienced high exposure to workplace discrimination, compared with workers with low exposure, had a higher hazard of hypertension (adjusted hazard ratio, 1.54 [95% CI, 1.11–2.13]). Sensitivity analysis excluding more baseline hypertension cases based on additional information on blood pressure plus antihypertensive medication use (N=975) showed slightly stronger associations. A trend analysis showed an exposure‐response association.

Conclusions

Workplace discrimination was prospectively associated with elevated risk of hypertension among US workers. The adverse impacts of discrimination on cardiovascular disease have major implications for workers' health and indicate a need for government and employer policy interventions addressing discrimination.
‘The Christic Models’
Authors: Kaunda Chammah J.
Published: April 2023

The task of this article is to interrogate and reconstruct Fabien Eboussi Boulaga’s theory of the Christic model as a pragmatic response to negative messianisms among African Pentecostals. Taking the hint from the two-natures messianic metaphoricity, the article argues that the messianic structure of Christ as an eternal myth is infinitely culturally demythologized into different historical and cultural contexts. It concludes by demonstrating that the two-natures messianicity captures a dialectic connection between divine infinity and human finitude. A pragmatic reconceptualization of the two-natures messianicity serves as a structuring principle or functional messianic model in the search for a balance of power and accountability in the struggle against menacing dominant hegemonies. It is critical because African Pentecostalism functions with the power-over as a structuring principle which makes congregants vulnerable to abuse of spiritual power and objectification, sexualization, and exploitation of a mystified mass.
The Year 2004: Historiographical Issues Concerning Islam in The Netherlands
Authors: Berger Maurits
Published: April 2023

This article aims to take a new approach to the study of Islam in Europe today by adopting a historiographical method with particular emphasis on the concept of temporality. The case-study will be The Netherlands, where it will be shown that a convergence of events in the year 2004 has contributed to the shaping of the national experience of ‘Islam’. It will be argued that these events, and the manner in which they took place, have created a national traumatic experience that has fossilised the way the Dutch have talked and thought about Islam since then.
The Time Has Arrived for Post-Post-Secularism
Authors: Shook John R.
Published: April 2023

Political secularism secures the disestablishment of religion by government. Religions sense an undemocratic power grab, and hence demand the disestablishment of secularism. The obvious options for political religion are few, to either promote populist majoritarianism, and/or control one or another branch of government. The options for political secularism in an age of secularization are no less momentous. Berlinerblau’s book Secularism: The Basics should be widely read as a clarion call for post-post-secularism, where political secularism has been all along. Post-secular academics and pro-religion intellectuals are getting called to account for their erosion of republican principles and their tacit or open endorsement of religious populism. The restoration of political secularism would counteract rising tides of conservative populism threatening constitutional democracy and human rights.
Secularism in Relation to Secularity and Secularization
Authors: Kasselstrand Isabella
Published: April 2023

My article offers commentary about Jacques Berlinerblau's new book Secularism: The Basics.
Commentary on Secularism: The Basics
Authors: De Nutte Niels
Published: April 2023

My article offers commentary about Jacques Berlinerblau's new book Secularism: The Basics.
Recognition of Non-Islamic Justice in the Works of Two Classical Islamic Political Thinkers: al-Ṭurṭūshī and Ibn Khaldūn
Authors: Ghossein Mohamad
Published: April 2023

The present paper examines the place of non-Islamic justice in two medieval Islamic political texts, Abū Bakr al-Ṭurṭūshī’s (d. 1126) Lamp of Kings (Sirāj al-Mulūk) and ʿAbd al-Raḥmān Ibn Khaldūn’s (d. 1406) Prolegomena (Muqaddima). By attempting to derive the principles of political justice through reason, and by repudiating claims that normative political values are solely derived from revelation, both thinkers commit to thoroughgoing recognition of non-Islamic forms of justice. As they formulate a pessimistic account of human nature, both contend that the principal objective of political justice is deterrence of innate human lawlessness. And since humans are deemed feeble and corruptible, they may settle for practical political solutions, even if outside the tradition of scripture and revelation. In the final analysis, this rationalist account is juxtaposed to the political aims of certain strands of modern traditionalism, where the role of reason is greatly minimized, if not completely subordinated to revelation. For fundamentalist revivalists, the categories of Islamic and non-Islamic societies intimate a striking opposition between just and unjust societies. While al-Ṭurṭūshī and Ibn Khaldūn also maintain a qualitative difference between Islamic and non-Islamic polities, their conclusions are markedly different from those of the modern traditionalists. The notion of justice they advance, which rests on a pessimistic thesis, results in a more intent recognition of non-Islamic political institutions and laws, irrespective of their creedal origins.
miR-140 Aggravates Chronic Obstructive Pulmonary Disease by Inducing Oxidative Stress in Diaphragm Cells by Targeting Nuclear Factor Erythroid 2-Related Factor 2
Authors: Ji Chaohui , Pan Huibin , Zhang Jie , Zhao Yiming , Fu Kai , Zhu Xiaofei
Published: April 2023

Our research proposes to explore the function of miR-140 in the development of COPD. A COPD model was established in rats, and bronchial epithelial cells (BECs) were extracted in the present study, followed by transfection with mimic NC+inhibitor NC, miR-144 mimic, and miR-144 inhibitor. Significantly higher apoptotic rate; downregulated Bcl-2; upregulated Bax and caspase-3; promoted production of IL-6, IL-1β, and MCP-1; higher ROS and MDA levels; lower SOD activity; and inactivated Nrf2 signaling were observed in miR-140 mimic-treated BECs, with opposite results observed in miR-140 inhibitor-treated BECs. The binding site between miR-140 and the 3′UTR region of Nrf2 was predicted and verified using the dual-luciferase gene reporter assay. COPD rats were administered with NC (agomir-NC and antagomir-NC), agomir-140, and antagomir-140. Compared to sham, dramatically lower pulmonary function, higher wet/dry value, severe pathological changes in lung tissues, higher release of inflammatory factors, enhanced apoptosis, higher ROS and MDA levels, lower SOD activity, and inactivated Nrf2 signaling were observed in the model and NC groups, which were greatly aggravated by agomir-140 and significantly reversed by antagomir-140. Collectively, our data suggest that miR-140 aggravates COPD by inducing OS in diaphragm cells by targeting Nrf2.
Construction of a New Protein–Protein Interaction and Molecular Biomarkers Networks in Alzheimer’s Disease Patients by Bioinformatics Screening
Authors: Chen Yanzhong , Zhou Huixuan , binYin Wen , Ren Hong
Published: April 2023

This study aimed to construct the molecular biomarkers of autophagy and endoplasmic reticulum stress (ERS), as well as their corresponding protein interaction network in Alzheimer’s disease (AD) patients with different levels of physical activity (PA) by bioinformatics methods.
The expression profiles of the genes were downloaded from Gene Expression Omnibus (GEO). Differentially expressed genes (DEGs) between AD samples with low, moderate and high levels of PA were studied. The autophagy and ERS-related genes (AERSRGs) were extracted from GeneCards and MsigDB databases.
The functional enrichment analysis was conducted to determine the function of DEGs. To explore the proteins, miRNAs and transcription factors (TF) interacting with DEGs, the protein–protein network, mRNA-miRNA network and mRNA-TF network were built using Cytoscape software. Then the
receiver operating characteristic (ROC) analysis were conducted to verify the diagnostic performance of hub genes. A total of 533 AERSRGs were identified in Group H and 150 AERSRGs were screened in Group M. Functional enrichment analysis suggested genes of AD play vital roles in some biological
process (e.g., cell cycle phase transition, mitochondrion organization, proteasomal protein catabolic process). KEGG enrichment analysis suggested that sarcopenia involves the pathways (e.g., GABA, P2Y receptors, serotonin release cycle). A total of 5 hub genes were screened in Group H and
9 were identified in Group M. ROC analysis suggested that several hub genes exhibited a relatively high sensitivity and specificity in both groups of AD. The hub genes screened in this study are closely correlated with autophagy and ERS in AD and can differentiate AD with different levels
of PA. SRC, MAPK3 and MAP2K1 exhibit relatively high sensitivity and specificity in diagnosis in Group H; MCM2, CDC42, HNRNPM, ASF1A, NCBP2, SNRNP70 and MCM6 exhibit relatively high sensitivity and specificity in diagnosis in Group M.
Serum-Derived Exosomes of Patients with Depression with Increased miR-96-5p Inhibit Neuronal Differentiation by Targeting RAC1
Authors: Wu Shengwei , Chen Zewei , Xuan Zhengzheng , Zhou Yufang , Zou Chubing , Wang Youtian , Ye Junrong , Zhao Pinge , Xiao Aixiang , Yu Lin , Guo Jianxiong
Published: April 2023

Exosomes are associated with neuronal differentiation in mental disorders, such as depression. This study explored the effects of exosomes on neuronal differentiation and their underlying molecular mechanisms. We isolated exosomes from the sera of patients with depression. These characteristics
were authenticated by TEM, NTA and western blotting. A differentiated cell model was established using all-trans retinoic acid (RA) to treat SH-SY5Y cells. Exosomes from depressed serum co-cultured with miR-96-5p mimic-transfected RA-treated cells, and PMA was used to activate RAC1. Neuronal
differentiation indices were analyzed by microscopy, quantitative PCR, immunofluorescence assay, and western blotting. The results illustrated that exosomes inhibited RA-induced differentiation, downregulated SYP, upregulated nestin, and decreased SYP-positive cell number. miR-96-5p was elevated
in serum-released exosomes from depressed patients, which impeded RA-induced neuronal differentiation. RAC1 is an miR-96-5p target. Activation of RAC1 partly counteracted the effects on neuronal differentiation induced by enhanced miR-96-5p levels. Additionally, decreasing miR-96-5p attenuated
depression-like behaviors and promoted hippocampal neuron differentiation induced by CUMS. Summarily, serum-derived exosomes from patients with depression suppress neuronal differentiation via the miR-96-5p/RAC1 axis. Moreover, decreased miR-96-5p levels suppresses CUMS-induced depression.
These consequences suggest that regulating exosomes secretion and exosomal miR-96-5p expression will be a new approach for therapy of depression.
Federalism in Russia After 1991
Authors: Srivastava Rachit
Published: April 2023

This article examines the federalization process and the spread of federalism in Russia after 1991. The Russian federal system has undergone several changes since the collapse of the Soviet Union. It arose as a result of several contracts signed between the federal government and the subjects. The federal structure of Russia is asymmetric and characterized by conflicts due to the system of division of power between the federal government and the subjects. The creation of an institutional mechanism was done to facilitate the application of the federal principles effectively. The President’s full control over political affairs, foreign policy and the economy can harm the successful development of the federal state in Russia.
Modeling the temperatures of traveler and ring in ring spinning
Authors: Wu Xi , Li Wenbin , Hurren Christopher , Wang Xungai
Published: April 2023

Ring spinning is the dominant system of manufacturing high-quality yarns in the textile industry. Spinning parameters affect the spinning tension distribution and consequently the temperatures of the ring/traveler system. Excessive traveler heat will limit the spindle speed and the efficiency of yarn production. This study modeled the effect of the traveler weight, yarn count, twist level and spindle speed on the temperatures of the traveler and ring during ring spinning. The finite element method was used to model the temperatures of the ring and the traveler for different spinning parameters. The experimental ring temperature and maximum traveler temperature from the validated model were recorded based on the experimental design. The results show that spindle speed, traveler weight and yarn count had a statistically significant effect on the temperatures of the traveler and the ring, with the spindle speed having the strongest effect. The twist level of yarn, within the range examined, had no statistically significant influence on the traveler and ring temperatures. For a given spindle speed, the traveler weight and yarn count have a significant effect on the maximum temperature of the traveler, and the temperature change is more pronounced at a higher spindle speed.
A comparative study for the main properties of silica and carbon black Filled bagasse-styrene butadiene rubber composites
Authors: Abdallah Khalaf Eyad Sayed
Published: April 2023

A comparative study has been done to investigate the effect of carbon black (CB) and fumed silica (Si) on the physico-mechanical and thermal properties of Bagasse/styrene-butadiene rubber (B-SBR) composites. Based on the incorporation of 25 phr from natural ground bagasse powder (GBP) as a supplement reinforcing filler in styrene butadiene rubber (SBR) vulcanizates, two identical sets of formulations have been prepared using different concentrations (20, 40, 60 and 80 phr) of carbon black and silica as the main reinforcing fillers in SBR composites. The ground bagasse powder (GBP) employed in this work, has a selective grain size distribution ranging from about 20–180 μm. As well, 2.5 phr of maleic anhydride (MA) was incorporated to improve the interfacial adhesion between SBR and bagasse. The distinguishing effect of different fillers on the rheological properties was clearly established. Tensile strength, elongation at break, modulus at 100% elongation (M 100), hardness (Shore A), abrasion loss, degree of swelling, as well as, thermal gravimetric analysis (TGA) of the rubber vulcanizates were studied. The prepared samples were morphologically analysed by scanning electron microscopy (SEM). The tensile strength and M 100% values of carbon black-filled compounds were obviously higher than those of the silica filled ones. In addition, hardness, wear and swelling properties were improved more and more by increasing the filler content. Whereas, the Si filled B/SBR showed a decreasing trend in elongation at break values but with an evidently higher plateau than that of CB filled ones. Overall, the CB filled vulcanizates recorded dominant mechanical properties compared to the Si filled vucanizates. On the other hand, both fillers offered a noticeable improvement in thermal stability, but with a preference favored for the silica filled compounds. Furthermore, the findings for SEM were found to be in agreement with the observed mechanical properties. This study summarizes a detailed discussion of the emerging green technologies for tyre production and depicted comprehensive data from the available literature.
Protection of melatonin treatment and combination with traditional antibiotics against septic myocardial injury
Authors: Di Wencheng , Jin Zhenxiao , Lei Wangrui , Liu Qiong , Yang Wenwen , Zhang Shaofei , Lu Chenxi , Xu Xiaoling , Yang Yang , Zhao Huadong
Published: April 2023

Background

Heart failure is a common complication of sepsis with a high mortality rate. It has been reported that melatonin can attenuate septic injury due to various properties. On the basis of previous reports, this study will further explore the effects and mechanisms of melatonin pretreatment, posttreatment, and combination with antibiotics in the treatment of sepsis and septic myocardial injury.

Methods and results

Our results showed that melatonin pretreatment showed an obvious protective effect on sepsis and septic myocardial injury, which was related to the attenuation of inflammation and oxidative stress, the improvement of mitochondrial function, the regulation of endoplasmic reticulum stress (ERS), and the activation of the AMPK signaling pathway. In particular, AMPK serves as a key effector for melatonin-initiated myocardial benefits. In addition, melatonin posttreatment also had a certain degree of protection, while its effect was not as remarkable as that of pretreatment. The combination of melatonin and classical antibiotics had a slight but limited effect. RNA-seq detection clarified the cardioprotective mechanism of melatonin.

Conclusion

Altogether, this study provides a theoretical basis for the application strategy and combination of melatonin in septic myocardial injury.
moBRCA-net: a breast cancer subtype classification framework based on multi-omics attention neural networks
Authors: Choi Joung Min , Chae Heejoon
Published: April 2023

Background

Breast cancer is a highly heterogeneous disease that comprises multiple biological components. Owing its diversity, patients have different prognostic outcomes; hence, early diagnosis and accurate subtype prediction are critical for treatment. Standardized breast cancer subtyping systems, mainly based on single-omics datasets, have been developed to ensure proper treatment in a systematic manner. Recently, multi-omics data integration has attracted attention to provide a comprehensive view of patients but poses a challenge due to the high dimensionality. In recent years, deep learning-based approaches have been proposed, but they still present several limitations.

Results

In this study, we describe moBRCA-net, an interpretable deep learning-based breast cancer subtype classification framework that uses multi-omics datasets. Three omics datasets comprising gene expression, DNA methylation and microRNA expression data were integrated while considering the biological relationships among them, and a self-attention module was applied to each omics dataset to capture the relative importance of each feature. The features were then transformed to new representations considering the respective learned importance, allowing moBRCA-net to predict the subtype.

Conclusions

Experimental results confirmed that moBRCA-net has a significantly enhanced performance compared with other methods, and the effectiveness of multi-omics integration and omics-level attention were identified. moBRCA-net is publicly available at https://github.com/cbi-bioinfo/moBRCA-net.
Carbon and nitrogen metabolism affects kentucky bluegrass rhizome expansion
Authors: Ran Fu , Yuan Yajuan , Bai Xiaoming , Li Changning , Li Juanxia , Chen Hui
Published: April 2023

Background

Rhizome is vital for carbon and nitrogen metabolism of the whole plant. However, the effect of carbon and nitrogen in the rhizome on rhizome expansion remains unclear.

Results

Three wild Kentucky bluegrass (Poa pratensis L.) germplasms with different rhizome expansion capacity (strong expansion capacity, ‘YZ’; medium expansion capacity, ‘WY’; and weak expansion capacity, ‘AD’) were planted in the field and the rhizomes number, tiller number, rhizome dry weight, physiological indicators and enzyme activity associated carbon and nitrogen metabolisms were measured. Liquid chromatography coupled to mass spectrometry (LC-MS) was utilized to analyze the metabolomic of the rhizomes. The results showed that the rhizome and tiller numbers of the YZ were 3.26 and 2.69-fold of that of the AD, respectively. The aboveground dry weight of the YZ was the greatest among all three germplasms. Contents of soluble sugar, starch, sucrose, NO3−-N, and free amino acid were significantly higher in rhizomes of the YZ than those of the WY and AD (P < 0.05). The activities of glutamine synthetase (GS), glutamate dehydrogenase (GDH) and sucrose phosphate synthase (SPS) of the YZ were the highest among all three germplasm, with values of 17.73 A·g− 1 h− 1, 5.96 µmol·g− 1 min− 1, and 11.35 mg·g− 1 h− 1, respectively. Metabolomics analyses revealed that a total of 28 differentially expressed metabolites (DEMs) were up-regulated, and 25 DEMs were down-regulated in both comparison groups (AD vs. YZ group and WY vs. YZ group). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis demonstrated that metabolites related to histidine metabolism, tyrosine metabolism, tryptophan metabolism, and phenylalanine metabolism were associated with rhizomes carbon and nitrogen metabolism.

Conclusions

Overall, the results suggest that soluble sugar, starch, sucrose, NO3−-N, and free amino acid in rhizome are important to and promote rhizome expansion in Kentucky bluegrass, while tryptamine, 3-methylhistidine, 3-indoleacetonitrile, indole, and histamine may be key metabolites in promoting carbon and nitrogen metabolism of rhizome.
Residual risk of mother-to-child transmission of HBV despite timely Hepatitis B vaccination: a major challenge to eliminate hepatitis B infection in Cambodia
Authors: E Bunthen , Ko Ko , Kim Rattana , Nagashima Shintaro , Ouoba Serge , Hussain Md Razeen Ashraf , Sato Tomoki , Chuon Channarena , Abe Kanon , Sugiyama Aya , Takahashi Kazuaki , Akita Tomoyuki , Tung Rathavy , Ork Vichit , Hossain Md. Shafiqul , Saphonn Vonthanak , Tanaka Junko
Published: April 2023

Background

In countries with intermediate or high hepatitis B virus (HBV) endemicity, mother-to-child transmission (MTCT) represents the main route of chronic HBV infection. There is a paucity of information on HBV MTCT in Cambodia. This study aimed to investigate the prevalence of HBV infection among pregnant women and its MTCT rate in Siem Reap, Cambodia.

Methods

This longitudinal study included two parts, study-1 to screen HBsAg among pregnant women and study-2 to follow up babies of all HBsAg-positive and one-fourth of HBsAg-negative mothers at their delivery and six-month post-partum. Serum or dried blood spot (DBS) samples were collected to examine HBV sero-markers by chemiluminescent enzyme immunoassay (CLEIA), and molecular analyses were performed on HBsAg-positive samples. Structured questionnaires and medical records were used to examine the risk factors for HBV infection. MTCT rate was calculated by HBsAg positivity of 6-month-old babies born to HBsAg-positive mothers and ascertained by the homology of HBV genomes in mother–child pair at 6-month-old.

Results

A total of 1,565 pregnant women were screened, and HBsAg prevalence was 4.28% (67/1565). HBeAg positivity was 41.8% and was significantly associated with high viral load (p < 0.0001). Excluding subjects who dropped out due to restrictions during COVID-19, one out of 35 babies born to HBsAg-positive mothers tested positive for HBsAg at 6 months of age, despite receiving timely HepB birth dose and HBIG, followed by 3 doses of HepB vaccine. Hence the MTCT rate was 2.86%. The mother of the infected baby was positive for HBeAg and had a high HBV viral load (1.2 × 109 copies/mL). HBV genome analysis showed 100% homology between the mother and the child.

Conclusions

Our findings illustrate the intermediate endemicity of HBV infection among pregnant women in Siem Reap, Cambodia. Despite full HepB vaccination, a residual risk of HBV MTCT was observed. This finding supports the recently updated guidelines for the prevention of HBV MTCT in 2021, which integrated screening and antiviral prophylaxis for pregnant women at risk of HBV MTCT. Furthermore, we strongly recommend the urgent implementation of these guidelines nationwide to effectively combat HBV in Cambodia.
Influence of herd immunity on norovirus: a long-term field study of repeated viral gastroenteritis outbreaks at the same facilities
Authors: Kumazaki Makoto , Usuku Shuzo
Published: April 2023

Background

Viral acute gastroenteritis (AG) is detected worldwide annually. Outbreaks caused by viruses associated with gastroenteritis have been reported repeatedly at the same facilities in Yokohama, Japan over several years. We investigated the statuses of these repeated outbreaks to consider herd immunity at the facility level.

Methods

Between September 2007 and August 2017, 1459 AG outbreaks were reported at 1099 facilities. Stool samples were collected for virological testing, and the norovirus gene was amplified and sequenced to determine the genotype using the N-terminal region of the capsid.

Results

The outbreaks were caused by norovirus, sapovirus, rotavirus A, and rotavirus C. Norovirus was consistently predominant over the 10-year period. Of 1099 facilities, 227 reported multiple outbreaks, of which norovirus-only combinations accounted for 76.2%. More outbreaks were due to different genotype combinations than the same genotype combinations. For facilities that experienced two norovirus outbreaks, the average interval between outbreaks was longer for groups with the same combinations than for groups with different genogroup or genotype combinations, although no statistically significant differences were observed. At 44 facilities, outbreaks occurred repeatedly during the same AG season, and most exhibited combinations of different norovirus genotypes or viruses. Among 49 combinations with the same norovirus genotype at the same facilities over 10 years, the most prevalent genotypes were combinations of genogroup II genotype 4 (GII.4), followed by GII.2, GII.6, GII.3, GII.14, and GI.3. The mean interval between outbreaks was 31.2 ± 26.8 months for all combinations, and the mean intervals were longer for non-GII.4 genotype cases than for GII.4 cases, and statistically significant differences were observed (t-test, P < 0.05). Additionally, these average intervals were longer for kindergarten/nursery schools and primary schools than for nursing homes for older adults (t-test, P < 0.05).

Conclusions

Repeated AG outbreaks at the same facilities in Yokohama during the 10-year study period included mainly norovirus combinations. Herd immunity at the facility level was maintained for at least the same AG season. Norovirus genotype-specific herd immunity was maintained for an average of 31.2 months during the study period, and these intervals differed depending on genotype.
Summary of the effect of an exercise intervention on antenatal depression and the optimal program: a systematic review and Meta-analysis
Authors: Liu Lanjuan , Liu Cheng , Liu Xiaotang , Yang Yang
Published: April 2023

Objective

This study aimed to examine the effect of exercise intervention for antenatal depression using meta-analysis and to propose the best exercise intervention program.

Methods

Review Manager 5.3 was used to analyze 17 papers with 2224 subjects by setting five moderators, including type, time, frequency, period, and format of exercise intervention, and a random-effects model was used to test for overall effect, heterogeneity, and publication bias.

Results

(1) The effect size of the exercise intervention on antenatal depression was d = -0.56, which reached a good effect and was statistically significant; b (2) The effect size of the exercise type on antenatal depression was Yoga and a combination of aerobic exercise in order of intervention; (3) the single intervention duration of 10–75 min all had a good effect on antenatal depression, and 30–60 min had the best effect; (4) the intervention frequency of 3 to 5 times/week had the greatest amount of intervention effect on maternal depression; (5) exercise lasting 6–10 weeks had a good intervention effect on antepartum depression, and the amount of effect decreased gradually with the extension of time; (6) In terms of exercise format, the amount of intervention effect on maternal depression was in the order of group exercise, individual + group exercise.

Conclusions

Exercise intervention can significantly alleviate antenatal depression symptoms. The best exercise program for exercise intervention for antenatal depression is: Yoga and a combination of aerobic exercise intervention effects are more prominent, and the intervention effect of Yoga is the best. The use of group exercise 3–5 times per week for 30–60 min for 6–10 weeks was more likely to achieve the desired intervention effect of improving antenatal depression.
Intra-observer reproducibility and inter-observer agreement of Fels skeletal age assessments among male tennis players 8–16 years
Authors: Sousa-e-Silva Paulo , Coelho-e-Silva Manuel J. , Celis-Moreno Jorge M. , Costa Daniela C. , Martinho Diogo V. , Ribeiro Luís P. , Oliveira Tomas , Gonçalves-Santos João , Tavares Oscar M. , Castanheira Joaquim M. , Pereira Telmo , Conde Jorge , Cayolla Ricardo R. , Duarte-Mendes Pedro , Myburgh Gillian K. , Cumming Sean P. , Malina Robert M.
Published: April 2023

Background

Skeletal age (SA) is an estimate of biological maturity status that is commonly used in sport-related medical examinations. This study considered intra-observer reproducibility and inter-observer agreement of SA assessments among male tennis players.

Methods

SA was assessed with the Fels method in 97 male tennis players with chronological ages (CA) spanning 8.7–16.8 years. Radiographs were evaluated by two independent trained observers. Based on the difference between SA and CA, players were classified as late, average or early maturing; if a player was skeletally mature, he was noted as such as an SA is not assigned.

Results

The magnitude of intra-individual differences between repeated SA assessments were d = 0.008 year (observer A) and d = 0.001 year (observer B); the respective coefficients of variation were 1.11% and 1.75%. Inter-observer mean differences were negligible (t = 1.252, p = 0.210) and the intra-class correlation coefficient was nearly perfect (ICC = 0.995). Concordance of classifications of players by maturity status between observers was 90%.

Conclusion

Fels SA assessments were highly reproducible and showed an acceptable level of inter-observer agreement between trained examiners. Classifications of players by skeletal maturity status based on assessments of the two observers were highly concordant, though not 100%. The results highlight the importance of experienced observers in skeletal maturity assessments.