bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Campus Tobacco-Free Policy on Tobacco and Electronic Nicotine Delivery Systems Initiation and Cessation among Students, Faculty, and Staff
Authors: Garcia Gabriel Macasiray , Mapaye Joy Chavez , Lopez Valeria Delgado , Roxbury Erin , Tabatabai-Yazdi Neelou
Published: January 2021

Objective: This study examines the effects of having a tobacco free policy in three University of Alaska campuses two years postimplementation among students, faculty, and staff. It also identifies factors associated with tobacco cessation and ENDS use.

Methods: Self-administered online survey was conducted in spring 2017 and 2018.

Results: Most of the current tobacco users began using tobacco prior to policy implementation, while most current ENDS users in 2018 started using ENDS when the policy had been in place. Students and those who are younger are more likely to quit tobacco during policy implementation; and males, students, and those who are younger and current tobacco users are more likely to use ENDS.

Conclusions: Study findings suggest that campus tobacco-free policy can promote tobacco cessation and help prevent tobacco initiation. However, such a policy does not necessarily affect ENDS use. Specific groups need additional interventions to assist in tobacco and ENDS cessation.
Genomic Instability and Cellular Senescence: Lessons From the Budding Yeast
Authors: Lee Jee Whu , Ong Eugene Boon Beng
Published: January 2021

Aging is a complex biological process that occurs in all living organisms. Aging is initiated by the gradual accumulation of biomolecular damage in cells leading to the loss of cellular function and ultimately death. Cellular senescence is one such pathway that leads to aging. The accumulation of nucleic acid damage and genetic alterations that activate permanent cell-cycle arrest triggers the process of senescence. Cellular senescence can result from telomere erosion and ribosomal DNA instability. In this review, we summarize the molecular mechanisms of telomere length homeostasis and ribosomal DNA stability, and describe how these mechanisms are linked to cellular senescence and longevity through lessons learned from budding yeast.
Niche Modulation of IGF-1R Signaling: Its Role in Stem Cell Pluripotency, Cancer Reprogramming, and Therapeutic Applications
Authors: Chen Pei-Chin , Kuo Yung-Che , Chuong Cheng-Ming , Huang Yen-Hua
Published: January 2021

Stem cells work with their niches harmoniously during development. This concept has been extended to cancer pathology for cancer stem cells (CSCs) or cancer reprogramming. IGF-1R, a classical survival signaling, has been shown to regulate stem cell pluripotency, CSCs, or cancer reprogramming. The mechanism underlying such cell fate determination is unclear. We propose the determination is due to different niches in embryo development and tumor malignancy which modulate the consequences of IGF-1R signaling. Here we highlight the modulations of these niche parameters (hypoxia, inflammation, extracellular matrix), and the targeted stem cells (embryonic stem cells, germline stem cells, and mesenchymal stem cells) and CSCs, with relevance to cancer reprogramming. We organize known interaction between IGF-1R signaling and distinct niches in the double-sided cell fate with emerging trends highlighted. Based on these new insights, we propose that, through targeting IGF-1R signaling modulation, stem cell therapy and cancer stemness treatment can be further explored.
Recent Progress in Quantitatively Monitoring Vesicular Neurotransmitter Release and Storage With Micro/Nanoelectrodes
Authors: Liu Yuying , Du Jinchang , Wang Mengying , Zhang Jing , Liu Chunlan , Li Xianchan
Published: January 2021

Exocytosis is one of the essential steps for chemical signal transmission between neurons. In this process, vesicles dock and fuse with the plasma membrane and release the stored neurotransmitters through fusion pores into the extracellular space, and all of these steps are governed with various molecules, such as proteins, ions, and even lipids. Quantitatively monitoring vesicular neurotransmitter release in exocytosis and initial neurotransmitter storage in individual vesicles is significant for the study of chemical signal transmission of the central nervous system (CNS) and neurological diseases. Electrochemistry with micro/nanoelectrodes exhibits great spatial–temporal resolution and high sensitivity. It can be used to examine the exocytotic kinetics from the aspect of neurotransmitters and quantify the neurotransmitter storage in individual vesicles. In this review, we first introduce the recent advances of single-cell amperometry (SCA) and the nanoscale interface between two immiscible electrolyte solutions (nanoITIES), which can monitor the quantity and release the kinetics of electrochemically and non-electrochemically active neurotransmitters, respectively. Then, the development and application of the vesicle impact electrochemical cytometry (VIEC) and intracellular vesicle impact electrochemical cytometry (IVIEC) and their combination with other advanced techniques can further explain the mechanism of neurotransmitter storage in vesicles before exocytosis. It has been proved that these electrochemical techniques have great potential in the field of neuroscience.
Information Structure and Scope Interactions: Disjunction Wide Scope Induced by Focus
Authors: Larralde Cécile , Konradt Alina , Szendrői Kriszta Eszter
Published: January 2021

In this paper we investigate the scopal reading of disjunctions in French negative sentences with pre-schoolers. We posit that the French disjunctor “ou” does not fit the traditional disjunction PPI/non-PPI dichotomy according to which a wide scope is taken by a PPI disjunction and a narrow scope when the disjunction is not a PPI. We hypothesized that focus could be a succesful scopal manipulator. Using Truth Value Judgment Tasks (TVJT), we tested French pre-schoolers' scopal reading of negated disjunctions in a neutral prosody condition and with prosodic focus on the disjunctor in a between subject design. We found that as predicted, prosodic focus often enduced participants to adopt a disjunction wide scope reading whereas a disjunction narrow scope reading was favored in the neutral prosody condition. This confirmed our hypothesis that focus can manipulate disjunction scope paramaters. It also shows that, when the disjunction is focalised, children have access to the disjunction wide scope reading earlier than previously thought. Finally, we can conclude that the distinction between PPI-disjunctor vs. non-PPI disjunctor languages needs to be more fine-grained.
Magnetic Properties of Plant Ashes and Their Influence on Magnetic Signatures of Fire in Soils
Authors: Till Jessica L. , Moskowitz Bruce , Poulton Simon W.
Published: January 2021

Fires are an integral part of many terrestrial ecosystems and have a strong impact on soil properties. While reports of topsoil magnetic enhancement after fires vary widely, recent evidence suggests that plant ashes provide the most significant source of magnetic enhancement after burning. To investigate the magnetic properties of burnt plant material, samples of individual plant species from Iceland and Germany were cleaned and combusted at various temperatures prior to rock magnetic and geochemical characterization. Mass-normalized saturation magnetization values for burnt plant residues increase with the extent of burning in nearly all samples. However, when normalized to the loss on ignition, fewer than half of ash and charcoal samples display magnetic enhancement relative to intact plant material. Thus, while magnetic mineral concentrations generally increase, changes in the total amount of magnetic material are much more variable. Elemental analyses of Icelandic samples reveal that both total plant Fe and saturation magnetization are strongly correlated with Ti and Al, indicating that most of the Fe-bearing magnetic phases originate from inorganic material such as soil and atmospheric dust. Electron microscopy confirmed that inorganic particulate matter remains on most plant surfaces after cleaning. Plants with more textured leaf surfaces retain more dust, and ash from these samples tend to exhibit higher saturation magnetization and metal concentrations. Magnetic properties of plant ash therefore result from the thermal transformation of Fe in both organic compounds and inorganic particulate matter, which become concentrated on a mass basis when organic matter is combusted. These results indicate that the soil magnetic response to burning will vary among sites and regions as a function of 1) fire intensity, 2) the local composition of dust and soil particles on leaf surfaces, and 3) vegetation type and consequent differences in leaf morphologies.
Precise Genome Editing in miRNA Target Site via Gene Targeting and Subsequent Single-Strand-Annealing-Mediated Excision of the Marker Gene in Plants
Authors: Ohtsuki Namie , Kizawa Keiko , Mori Akiko , Nishizawa-Yokoi Ayako , Komatsuda Takao , Yoshida Hitoshi , Hayakawa Katsuyuki , Toki Seiichi , Saika Hiroaki
Published: January 2021

Gene targeting (GT) enables precise genome modification—e.g., the introduction of base substitutions—using donor DNA as a template. Combined with clean excision of the selection marker used to select GT cells, GT is expected to become a standard, generally applicable, base editing system. Previously, we demonstrated marker excision via a piggyBac transposon from GT-modified loci in rice. However, piggyBac-mediated marker excision has the limitation that it recognizes only the sequence TTAA. Recently, we proposed a novel and universal precise genome editing system consisting of GT with subsequent single-strand annealing (SSA)-mediated marker excision, which has, in principle, no limitation of target sequences. In this study, we introduced base substitutions into the microRNA miR172 target site of the OsCly1 gene—an ortholog of the barley Cleistogamy1 gene involved in cleistogamous flowering. To ensure efficient SSA, the GT vector harbors 1.2-kb overlapped sequences at both ends of a selection marker. The frequency of positive–negative selection-mediated GT using the vector with overlapped sequences was comparable with that achieved using vectors for piggyBac-mediated marker excision without overlapped sequences, with the frequency of SSA-mediated marker excision calculated as ~40% in the T0 generation. This frequency is thought to be adequate to produce marker-free cells, although it is lower than that achieved with piggyBac-mediated marker excision, which approaches 100%. To date, introduction of precise substitutions in discontinuous multiple bases of a targeted gene using base editors and the prime editing system based on CRISPR/Cas9 has been quite difficult. Here, using GT and our SSA-mediated marker excision system, we succeeded in the precise base substitution not only of single bases but also of artificial discontinuous multiple bases in the miR172 target site of the OsCly1 gene. Precise base substitution of miRNA target sites in target genes using this precise genome editing system will be a powerful tool in the production of valuable crops with improved traits.
Dissecting the Invasion-Associated Long Non-coding RNAs Using Single-Cell RNA-Seq Data of Glioblastoma
Authors: Pang Bo , Quan Fei , Ping Yanyan , Hu Jing , Lan Yujia , Pang Lin
Published: January 2021

Glioblastoma (GBM) is characterized by rapid and lethal infiltration of brain tissue, which is the primary cause of treatment failure and deaths for GBM. Therefore, understanding the molecular mechanisms of tumor cell invasion is crucial for the treatment of GBM. In this study, we dissected the single-cell RNA-seq data of 3345 cells from four patients and identified dysregulated genes including long non-coding RNAs (lncRNAs), which were involved in the development and progression of GBM. Based on co-expression network analysis, we identified a module (M1) that significantly overlapped with the largest number of dysregulated genes and was confirmed to be associated with GBM invasion by integrating EMT signature, experiment-validated invasive marker and pseudotime trajectory analysis. Further, we denoted invasion-associated lncRNAs which showed significant correlations with M1 and revealed their gradually increased expression levels along the tumor cell invasion trajectory, such as VIM-AS1, WWTR1-AS1, and NEAT1. We also observed the contribution of higher expression of these lncRNAs to poorer survival of GBM patients. These results were mostly recaptured in another validation data of 7930 single cells from 28 GBM patients. Our findings identified lncRNAs that played critical roles in regulating or controlling cell invasion and migration of GBM and provided new insights into the molecular mechanisms underlying GBM invasion as well as potential targets for the treatment of GBM.
A Decision-Making Tool for Planning O&M Activities of Offshore Wind Farms Using Simulated Actual Decision Drivers
Authors: Tomaselli Pietro D. , Dixen Martin , Bolaños Sanchez Rodolfo , Sørensen Jacob Tornfeldt
Published: January 2021

Safe and cost-efficient planning Operation&Maintenance (O&M) activities for the turbines of Offshore Wind Farms is crucial for the offshore wind industry. The execution of the planned tasks depends on the workability at sea. Workability assessments aim to find time periods, called weather windows, during which the personnel can execute the job at hand safely. Traditionally, weather windows analyses are based on thresholds applied on relevant metocean conditions in the area of interest, commonly wave height, wave period and wind speed. In this way, tasks are planned in windows during which the forecast metocean conditions do not exceed the defined thresholds. This paper presents a numerical tool that provides weather windows based on more direct measures of workability, that is seasickness on board during the trip to the turbines and bow motions, which endanger crew transfers from vessel to turbine. When assessing weather windows, such parameters better describe the actual decision drivers in a real operational setting than mere metocean thresholds, which are, in practical cases, discretionally judged by the O&M operator upon experience. Therefore, the reliability of workability predictions can increase, leading to financial gains for the wind industry and safer environment for O&M operators. The paper shows an application of the tool, where a full O&M scenario is simulated. The scenario comprises the transit from the port to the offshore site, the work carried out on the turbine and the transit back to the port. In particular, the application highlights the key capability of the tool of calculating vessel motions, which are elaborated to produce weather windows. With its low computational time-demand, the tool aims to support the decision-making processes that produce short- and long-term O&M plans.
Antagonistic Control of Genetic Circuit Performance for Rapid Analysis of Targeted Enzyme Activity in Living Cells
Authors: Kwon Kil Koang , Kim Haseong , Yeom Soo-Jin , Rha Eugene , Lee Jinju , Lee Hyewon , Lee Dae-Hee , Lee Seung-Goo
Published: January 2021

Genetic circuits have been developed for quantitative measurement of enzyme activity, metabolic engineering of strain development, and dynamic regulation of microbial cells. A genetic circuit consists of several bio-elements, including enzymes and regulatory cassettes, that can generate the desired output signal, which is then used as a precise criterion for enzyme screening and engineering. Antagonists and inhibitors are small molecules with inhibitory effects on regulators and enzymes, respectively. In this study, an antagonist and an inhibitor were applied to a genetic circuit for a dynamic detection range. We developed a genetic circuit relying on regulators and enzymes, allowing for straightforward control of its output signal without additional genetic modification. We used para-nitrophenol and alanine as an antagonist of DmpR and inhibitor of tyrosine phenol-lyase, respectively. We show that the antagonist resets the detection range of the genetic circuit similarly to a resistor in an electrical logic circuit. These biological resistors in genetic circuits can be used as a rapid and precise controller of variable outputs with minimal circuit configuration.
Myelin Oligodendrocyte Glycoprotein (MOG) Antibody Positive Patients in a Multi-Ethnic Canadian Cohort
Authors: Cross Helen , Sabiq Farahna , Ackermans Nathalie , Mattar Andrew , Au Shelly , Woodhall Mark , Sun Bo , Devonshire Virginia , Carruthers Robert , Sayao Ana Luiza , Bhan Virender , Schabas Alice , Chan Jillian , Fritzler Marvin , Waters Patrick , Traboulsee Anthony
Published: January 2021

Introduction: Myelin oligodendrocyte glycoprotein (MOG) antibody-associated disease is a recently described central nervous system (CNS) inflammatory disorder with phenotypic overlap with Neuromyelitis Optica Spectrum Disorder (NMOSD). NMOSD seronegative patients, and those with limited forms of the disorder, become suspects for MOG antibody-associated disease. We describe a multi-ethnic population with MOG antibody seropositivity from the University of British Columbia MS/NMO clinic.

Methods: AQP4-antibody seronegative patients presenting 2005–2016 with CNS inflammatory disease suspicious for NMOSD, as well as 20 MS controls, were retrospectively tested for MOG-IgG1 antibodies by live cell-based assay at Oxford Autoimmune Neurology Diagnostic Laboratory (UK) and by a commercial fixed cell-based assay at MitogenDx (Calgary, Canada). Additional MOG seropositive cases were identified through routine clinical interaction (2016–2018) using one of these laboratories. Clinical data was reviewed retrospectively.

Results: Retrospective testing identified 21 MOG seropositives (14 by live assay only, 3 by fixed assay only and 4 by both) representing 14% of the “NMOSD suspects” cohort. One multiple sclerosis (MS) control serum was MOG seropositive. Twenty additional MOG positive cases were identified prospectively. Of 42 patients (27 female), median disease onset age was 29 years (range 3–62; 9 pediatric cases), 20 (47%) were non-Caucasian, and 3 (7%) had comorbid autoimmune disease. Most common onset phenotypes were optic neuritis (23, 55%; 8 bilateral) and myelitis (9, 21%; 6 longitudinally extensive) Three of the patients in our cohort experienced cortical encephalitis; two presented with seizures. Onset was moderate-severe in 64%, but 74% had good response to initial steroid therapy. Cumulative relapse probability for the MOG positive group at 1 year was 0.428 and at 4 years was 0.628. Most had abnormal brain imaging, including cortical encephalitis and poorly demarcated subcortical and infratentorial lesions. Few “classic MS” lesions were seen. Optic nerve lesions (frequently bilateral) were long and predominantly anterior, but 5 extended to the chiasm. Spinal cord lesions were long and short, with involvement of multiple spinal regions simultaneously, including the conus medullaris.

Conclusions: Our MOG seropositive patients display phenotypes similar to previous descriptions, including cortical lesions with seizures and conus medullaris involvement. Many patients relapsed, predominantly in a different CNS location from onset. Serologic data from two different cell-based antibody assays highlight the discrepancies between live and fixed testing for MOG antibodies.
Edible Crickets (Orthoptera) Around the World: Distribution, Nutritional Value, and Other Benefits—A Review
Authors: Magara Henlay J. O. , Niassy Saliou , Ayieko Monica A. , Mukundamago Mukundi , Egonyu James P. , Tanga Chrysantus M. , Kimathi Emily K. , Ongere Jackton O. , Fiaboe Komi K. M. , Hugel Sylvain , Orinda Mary A. , Roos Nanna , Ekesi Sunday
Published: January 2021

Edible crickets are among the praised insects that are gaining recognition as human food and livestock feed with a potential of contributing to food security and reduction of malnutrition. Globally, the sustainable use of crickets as food or feed is undermined by lack of information on the number of the edible crickets, the country where they are consumed, and the developmental stages consumed. Furthermore, lack of data on their nutritional content and the potential risks to potential consumers limits their consumption or inclusion into other food sources. We reviewed published literature on edible cricket species, countries where they are consumed, and the stage at which they are consumed. We further reviewed information on their nutritional content, the safety of cricket consumption, and the sensory qualities of the edible crickets. We also looked at other benefits derived from the crickets, which include ethnomedicine, livestock feed, pest management strategies, contribution to economic development, and livelihood improvement, particularly in terms of use as food preservatives and use within music, sports, and cultural entomology. Lastly, we reviewed information on the farming of edible crickets. In this review, we report over 60 cricket species that are consumed in 49 countries globally. Nutritionally, crickets are reported to be rich in proteins, ranging from 55 to 73%, and lipids, which range from 4.30 to 33.44% of dry matter. The reported amount of polyunsaturated fatty acids (PUFA) is 58% of the total fatty acids. Edible crickets contain an appreciable amount of macro- and micro-mineral elements such as calcium, potassium, magnesium, phosphorus, sodium, iron, zinc, manganese, and copper. Also, the crickets are rich in the required amount of vitamins such as B group vitamins and vitamins A, C, D, E, and K. Overall, the cricket species examined in this review are safe to be consumed, and they display high proximate content that can replace plant and livestock products. The crickets play valuable roles in contributing to the economies of many countries and livelihoods, and they have medicinal and social benefits. This review is expected to promote greater recognition of crickets as a source of food, feed, and other benefits in the world and encourage up-scaling by farming them for sustainable utilization.
An Updated Review of the Diagnostic Methods in Delayed Drug Hypersensitivity
Authors: Copaescu Ana , Gibson Andrew , Li Yueran , Trubiano Jason A. , Phillips Elizabeth J.
Published: January 2021

Delayed drug hypersensitivity reactions are clinically diverse reactions that vary from isolated benign skin conditions that remit quickly with no or symptomatic treatment, drug discontinuation or even continued drug treatment, to the other extreme of severe cutaneous adverse reactions (SCARs) that are associated with presumed life-long memory T-cell responses, significant acute and long-term morbidity and mortality. Diagnostic “in clinic” approaches to delayed hypersensitivity reactions have included patch testing (PT), delayed intradermal testing (IDT) and drug challenges for milder reactions. Patch and IDT are, in general, performed no sooner than 4–6 weeks after resolution of the acute reaction at the maximum non-irritating concentrations. Functional in vitro and ex vivo assays have largely remained the province of research laboratories and include lymphocyte transformation test (LTT) and cytokine release enzyme linked ImmunoSpot (ELISpot) assay, an emerging diagnostic tool which uses cytokine release, typically IFN-γ, after the patient’s peripheral blood mononuclear cells are stimulated with the suspected drug(s). Genetic markers such as human leukocyte antigen have shown recent promise for both pre-prescription screening as well as pre-emptive and diagnostic testing strategies.
Contamination of Aflatoxins Induces Severe Hepatotoxicity Through Multiple Mechanisms
Authors: Hua Zhenglai , Liu Rui , Chen Youwen , Liu Guangzhi , Li Chenxi , Song Yurong , Cao Zhiwen , Li Wen , Li Weifeng , Lu Cheng , Liu Yuanyan
Published: January 2021

Aflatoxins (AFs) are commonly contaminating mycotoxins in foods and medicinal materials. Since they were first discovered to cause “turkey X” disease in the United Kingdom in the early 1960s, the extreme toxicity of AFs in the human liver received serious attention. The liver is the major target organ where AFs are metabolized and converted into extremely toxic forms to engender hepatotoxicity. AFs influence mitochondrial respiratory function and destroy normal mitochondrial structure. AFs initiate damage to mitochondria and subsequent oxidative stress. AFs block cellular survival pathways, such as autophagy that eliminates impaired cellular structures and the antioxidant system that copes with oxidative stress, which may underlie their high toxicities. AFs induce cell death via intrinsic and extrinsic apoptosis pathways and influence the cell cycle and growth via microribonucleic acids (miRNAs). Furthermore, AFs induce the hepatic local inflammatory microenvironment to exacerbate hepatotoxicity via upregulation of NF-κB signaling pathway and inflammasome assembly in the presence of Kupffer cells (liver innate immunocytes). This review addresses the mechanisms of AFs-induced hepatotoxicity from various aspects and provides background knowledge to better understand AFs-related hepatoxic diseases.
Mapping and Validation of Stem Rust Resistance Loci in Spring Wheat Line CI 14275
Authors: Kosgey Zennah C. , Edae Erena A. , Dill-Macky Ruth , Jin Yue , Bulbula Worku Denbel , Gemechu Ashenafi , Macharia Godwin , Bhavani Sridhar , Randhawa Mandeep S. , Rouse Matthew N.
Published: January 2021

Stem rust caused by Puccinia graminis f. sp. tritici (Pgt) remains a constraint to wheat production in East Africa. In this study, we characterized the genetics of stem rust resistance, identified QTLs, and described markers associated with stem rust resistance in the spring wheat line CI 14275. The 113 recombinant inbred lines, together with their parents, were evaluated at the seedling stage against Pgt races TTKSK, TRTTF, TPMKC, TTTTF, and RTQQC. Screening for resistance to Pgt races in the field was undertaken in Kenya, Ethiopia, and the United States in 2016, 2017, and 2018. One gene conferred seedling resistance to race TTTTF, likely Sr7a. Three QTL were identified that conferred field resistance. QTL QSr.cdl-2BS.2, that conferred resistance in Kenya and Ethiopia, was validated, and the marker Excalibur_c7963_1722 was shown to have potential to select for this QTL in marker-assisted selection. The QTL QSr.cdl-3B.2 is likely Sr12, and QSr.cdl-6A appears to be a new QTL. This is the first study to both detect and validate an adult plant stem rust resistance QTL on chromosome arm 2BS. The combination of field QTL QSr.cdl-2BS.2, QSr.cdl-3B.2, and QSr.cdl-6A has the potential to be used in wheat breeding to improve stem rust resistance of wheat varieties.
Are There Any Cognitive Benefits of Computer-Based Foreign Language Training for Healthy Elderly People? – A Mini-Review
Authors: Klimova Blanka
Published: January 2021

The purpose of this mini-review is to investigate if there are any cognitive benefits of computer-based foreign language training for healthy older individuals aged 55+ years. The author conducted a literature search of peer-reviewed English written research articles found in Pub Med, Web of Science and Scopus. The findings of this mini-review reveal that the research on the cognitive benefits of computer-based foreign language training for healthy older individuals is small-scale. The limited research findings of only three relevant studies indicate that these computer-based foreign language training programs may bring cognitive benefits for healthy elderly people, especially as far as the enhancement of their cognitive functions such as working memory are concerned. In addition, the authors of these studies suggest that foreign language learning is a useful activity for healthy older adults since it has the benefits of being meaningful (an advantage over other cognitive training approaches) and provides the chance for acquiring important skills that can benefit other aspects of life, such as travel or communication. In conclusion, the author of this mini-review also provides several implications for practice and future research.
How to Help Young Children Ask Better Questions?
Authors: Ruggeri Azzurra , Walker Caren M. , Lombrozo Tania , Gopnik Alison
Published: January 2021

In this paper, we investigate the informativeness of 4- to 6-year-old (N = 125) children’s questions using a combined qualitative and quantitative approach. Children were presented with a hierarchical version of the 20-questions game, in which they were given an array of objects that could be organized into three category levels based on shared features. We then tested whether it is possible to scaffold children’s question-asking abilities without extensive training. In particular, we supported children’s categorization performance by providing the object-related features needed to ask effective constraint-seeking questions. We found that with both age and scaffolding children asked more effective questions, targeting higher category levels and therefore reaching the solution with fewer questions. We discuss the practical and theoretical implications of these results.
Compulsive Internet Pornography Use and Mental Health: A Cross-Sectional Study in a Sample of University Students in the United States
Authors: Camilleri Christina , Perry Justin T. , Sammut Stephen
Published: January 2021

Background

The sustained rise in negative mental health reports among university students is a source of continued global concern, and investigation continues into potential contributors to this rise. This includes the increased prevalence of risky sexual behaviors. Related is the increased prevalence of pornography use. Our study sought to explore the potential relationship between compulsive use of pornography and mental health in university students.

Methods

Our sample consisted of university students (N = 1031; 34% male, 66% female) from Franciscan University of Steubenville, Steubenville, Ohio. An anonymous survey was sent to all students at the university over the age of 18. The survey was comprised of the following: (1) demographic questions, (2) questions on pornography use and perception, (3) a modified version of the Compulsive Internet Use Scale (mCIUS) assessing various factors associated with compulsive internet pornography use, (4) questions assessing emotional and sexual states relative to pornography use (EmSS), and (5) the 21-question version of the Depression, Anxiety and Stress Scale (DASS-21).

Results

Our results indicate that 56.6% of those surveyed reported lifetime pornography use, with a significantly higher proportion of males than females reporting such use. The majority of students reported accessing pornography through internet-related technologies. Additionally, 17.0, 20.4, and 13.5% of students reported severe or extremely severe levels of depression, anxiety and stress, respectively, with compulsive pornography use significantly affecting all three mental health parameters in both sexes. Exploratory Factor Analysis identified three factors suggesting emotional coping, dependence and preoccupation for the mCIUS items and three factors reflecting interoceptive, impotent, and extrinsic characteristics for the EmSS items. Regression analysis indicated that various demographics, items pertaining to reduced control and social impairment, and other variables pertaining to pornography use predicted mental health outcomes. Faith, morals and personal motivation were the primary variables reported to help reduce pornography use.

Conclusion

Our analyses indicate a significant relationship between mental health and pornography use, including behaviors reflecting behavioral addictions, highlighting the necessity for a better understanding and consideration of the potential contribution of internet pornography to negative mental health among university students.
A Rasch Analysis of the Irrational Procrastination Scale (IPS)
Authors: Shaw Amy , Zhang Jennifer J.
Published: January 2021

The present study analyses the psychometric properties of the irrational procrastination scale (IPS; Steel, 2002, 2010) in a sample of United States college students using the Rasch modeling approach. Results showed that the IPS items had a high level of reliability, good content validity, structural validity, and substantive validity, and no differential item functioning (DIF) effects in terms of gender. The IPS was found to be unidimensional, supporting the originally proposed theoretical structure by Steel (2002, 2010). Finally, psychometric implications derived from the results and study limitations are discussed; recommendations for future investigations are also offered.
Impact of N-Acetylcysteine on the Gut Microbiota in the Piglets Infected With Porcine Epidemic Diarrhea Virus
Authors: Wu Tao , Lyu Yang , Li Xueni , Wu Mengjun , Yu Kui , Li Siyuan , Ji Changzheng , Zhang Qian , Zhang Yanyan , Zhao Di , Yi Dan , Hou Yongqing
Published: January 2021

This study was to investigate the impact of N-acetylcysteine (NAC) on the gut microbiota in the healthy piglets and the piglets infected with porcine epidemic diarrhea virus (PEDV). Forty seven-day-old piglets were allocated into four groups: control group, NAC group (supplemented with 50 mg/kg body weight NAC), PEDV group (inoculated with 104.5 TCID50 PEDV), and PEDV+NAC group (PEDV infection + NAC supplementation). The intestinal content was collected for DNA extraction and Illumina sequencing. The PEDV-infected piglets displayed distinct bacterial communities compared to the healthy piglets. PEDV infection decreased the abundance of Shigella and increased the abundance of Lactobacillus, Odoribacter, Anaerovibrio, Helicobacter, unclassified Lachnospiraceae, and Sutterella; affected several functions associated with metabolism, barrier, and immune. NAC supplementation decreased the abundance of unclassified Rikenellaceae and increased the abundance of Lactobacillus, Streptococcus, and Enterococcus in the healthy piglets, decreased the abundance of Oscillospira and Prevotella and increased the abundance of Lactobacillus in the PEDV-infected piglets; altered multiple functions involving in amino acid metabolism, cell signaling, cellular community, disease-related pathways, endocrine, and excretory system. In conclusion, PEDV infection caused severe dysbiosis of gut microbiome, whereas NAC supplementation played a positive role in regulating the gut microbiome during PEDV infection. Therefore, substances that can regulate gut microbiota could be ideal candidates to prevent or treat PEDV infection.
Understanding the Adoption of Innovations in Agriculture: A Review of Selected Conceptual Models
Authors: Montes de Oca Munguia Oscar , Pannell David J. , Llewellyn Rick
Published: January 2021

Models can provide a structured way to think about adoption and provide a method to investigate the impacts of different factors in the adoption process. With at least 70 years of research in the adoption of agricultural innovations, there has been a proliferation of adoption models, both conceptual and numerical. This diversity has resulted in a lack of convergence in the way adoption is defined, explained, and measured, causing agricultural extension and policy to rely on a body of literature that is often not able to offer clear recommendations on the variables or mechanisms that can be used to design interventions. We conducted a review of conceptual models to clarify the concepts and approaches used in the practice of modeling adoption in agriculture. We described general adoption conceptual models originating from sociology, psychology, economics, and marketing and reviewed examples of models specifically defined for the study of adoption in agriculture. We also broadly assessed the ability of conceptual models to support building numerical models. Our review covered a range of modeling approaches for diffusion and individual adoption, illustrating different perspectives used in the literature. We found that key elements that should be used in adoption models for agriculture include: a way to assess the performance of the proposed new technology (e.g., relative advantage, both economic and non-economic) in relation to the existing technology or practice in place, the process of learning about this advantage, the interaction between individual decision-making and external influences, and characteristics of potential adopters affecting their attitudes towards the technology. We also detected inconsistencies in how different elements are treated in different conceptual models, particularly behavioral elements such as attitudes, motivations, intentions, and external influences. In terms of modeling, the main implication of these inconsistencies is the difficulty to generate quantitative evidence to support these models since multiple interpretations make it difficult to achieve consistency in the definition of observable, measurable variables that can be used to quantify cause-effect relationships. Suggestions for further research in the field include: questioning whether the adoption of all technologies and practices can be represented by the same adoption or learning process, exploring the dynamics in the relationship between adopters and technology before and after adoption, and questioning the basic assumptions behind the process of individual decision-making models and the role of collective decision-making. Findings from this review can be considered by adoption researchers and modelers in their work to assist policy and extension efforts to improve the uptake of future beneficial agricultural innovations.
Exposure and Health Impacts Related to Outdoor and Indoor Air Pollutants
Authors: Khwaja Haider A.
Published: January 2021

The five papers included in this Special Issue represent a diverse selection of contributions [...]
Perlecan in the Natural and Cell Therapy Repair of Human Adult Articular Cartilage: Can Modifications in This Proteoglycan Be a Novel Therapeutic Approach?
Authors: Garcia John , McCarthy Helen S. , Kuiper Jan Herman , Melrose James , Roberts Sally
Published: January 2021

Articular cartilage is considered to have limited regenerative capacity, which has led to the search for therapies to limit or halt the progression of its destruction. Perlecan, a multifunctional heparan sulphate (HS) proteoglycan, promotes embryonic cartilage development and stabilises the mature tissue. We investigated the immunolocalisation of perlecan and collagen between donor-matched biopsies of human articular cartilage defects (n = 10 × 2) that were repaired either naturally or using autologous cell therapy, and with age-matched normal cartilage. We explored how the removal of HS from perlecan affects human chondrocytes in vitro. Immunohistochemistry showed both a pericellular and diffuse matrix staining pattern for perlecan in both natural and cell therapy repaired cartilage, which related to whether the morphology of the newly formed tissue was hyaline cartilage or fibrocartilage. Immunostaining for perlecan was significantly greater in both these repair tissues compared to normal age-matched controls. The immunolocalisation of collagens type III and VI was also dependent on tissue morphology. Heparanase treatment of chondrocytes in vitro resulted in significantly increased proliferation, while the expression of key chondrogenic surface and genetic markers was unaffected. Perlecan was more prominent in chondrocyte clusters than in individual cells after heparanase treatment. Heparanase treatment could be a means of increasing chondrocyte responsiveness to cartilage injury and perhaps to improve repair of defects.
Functional Assembly of Caenorhabditis elegans Cytochrome b-2 (Cecytb-2) into Phospholipid Bilayer Nanodisc with Enhanced Iron Reductase Activity
Authors: Abosharaf Hamed A. , Sakamoto Yuki , Radwan Aliaa M. , Yuzu Keisuke , Fujimura Mika , Diab Thoria , Mohamed Tarek M. , Chatani Eri , Kimura Tetsunari , Tsubaki Motonari
Published: January 2021

Among seven homologs of cytochrome b561 in a model organism C. elegans, Cecytb-2 was confirmed to be expressed in digestive organs and was considered as a homolog of human Dcytb functioning as a ferric reductase. Cecytb-2 protein was expressed in Pichia pastoris cells, purified, and reconstituted into a phospholipid bilayer nanodisc. The reconstituted Cecytb-2 in nanodisc environments was extremely stable and more reducible with ascorbate than in a detergent-micelle state. We confirmed the ferric reductase activity of Cecytb-2 by analyzing the oxidation of ferrous heme upon addition of ferric substrate under anaerobic conditions, where clear and saturable dependencies on the substrate concentrations following the Michaelis–Menten equation were observed. Further, we confirmed that the ferric substrate was converted to a ferrous state by using a nitroso-PSAP assay. Importantly, we observed that the ferric reductase activity of Cecytb-2 became enhanced in the phospholipid bilayer nanodisc.
The Moral Foundations of Child Health and Social Policies: A Critical Interpretive Synthesis
Authors: Denburg Avram E. , Giacomini Mita , Ungar Wendy J. , Abelson Julia
Published: January 2021

Background: Allusions to the uniqueness and value of childhood abound in academic, lay, and policy discourse. However, little clarity exists on the values that guide child health and social policy-making. We review extant academic literature on the normative dimensions of child health and social policy to provide foundations for the development of child-focused public policies. Methods: We conducted a critical interpretive synthesis of academic literature on the normative dimensions of child health and social policy-making. We employed a social constructivist lens to interpret emergent themes. Political theory on the social construction of target populations served as a bridge between sociologies of childhood and public policy analysis. Results: Our database searches returned 14,658 unique articles; full text review yielded 72 relevant articles. Purposive sampling of relevant literature complemented our electronic searches, adding 51 original articles, for a total of 123 articles. Our analysis of the literature reveals three central themes: potential, rights, and risk. These themes retain relevance in diverse policy domains. A core set of foundational concepts also cuts across disciplines: well-being, participation, and best interests of the child inform debate on the moral and legal dimensions of a gamut of child social policies. Finally, a meta-theme of embedding encompasses the pervasive issue of a child’s place, in the family and in society, which is at the heart of much social theory and applied analysis on children and childhood. Conclusions: Foundational understanding of the moral language and dominant policy frames applied to children can enrich analyses of social policies for children. Most societies paint children as potent, vulnerable, entitled, and embedded. It is the admixture of these elements in particular policy spheres, across distinct places and times, that often determines the form of a given policy and societal reactions to it. Subsequent work in this area will need to detail the degree and impact of variance in the values mix attached to children across sociocultural contexts and investigate tensions between what are and what ought to be the values that guide social policy development for children.
Electrical Circuits RC, LC, and RLC under Generalized Type Non-Local Singular Fractional Operator
Authors: Acay Bahar , Inc Mustafa
Published: January 2021

The current study is of interest when performing a useful extension of a crucial physical problem through a non-local singular fractional operator. We provide solutions that include three arbitrary parameters α, ρ, and γ for the Resistance-Capacitance (RC), Inductance-Capacitance (LC), and Resistance-Inductance-Capacitance (RLC) electric circuits utilizing a generalized type fractional operator in the sense of Caputo, called non-local M-derivative. Additionally, to keep the dimensionality of the physical parameter in the proposed model, we use an auxiliary parameter. Owing to the fact that all solutions depend on three parameters unlike the other solutions containing one or two parameters in the literature, the solutions obtained in this study have more general results. On the other hand, in order to observe the advantages of the non-local M-derivative, a comprehensive comparison is carried out in the light of experimental data. We make this comparison for the RC circuit between the non-local M-derivative and Caputo derivative. It is clearly shown on graphs that the fractional M-derivative behaves closer to the experimental data thanks to the added parameters α, ρ, and γ.
A Survey of Dentists’ Perception of Chair-Side CAD/CAM Technology
Authors: Nassani Mohammad Zakaria , Ibraheem Shukran , Shamsy Enass , Darwish Mahmoud , Faden Asmaa , Kujan Omar
Published: January 2021

The application of computer-aided design and computer-aided manufacturing (CAD/CAM) technology in dentistry has rapidly expanded. This survey aimed to investigate attitudes and current practice of dentists in Riyadh, Saudi Arabia regarding chair-side CAD/CAM technology. An online questionnaire was prepared and sent to a convenience sample of dentists in Riyadh. Questions related to practice aspects of chair-side CAD/CAM system, attitudes and training were presented. A total of 114 questionnaires were completed (77.6% response rate). Study population comprised dentists of a wide range of clinical experience who are working in the various regions of Riyadh city, Saudi Arabia. Of the participants, 27.2% indicated the presence of a chair-side CAD/CAM system at their current workplace and 57% used the chair-side CAD/CAM in the fabrication of dental crowns. The vast majority of participants (81%) believe that the overall quality of chair-side CAD/CAM restorations is at least as good as those fabricated by a lab technician or much better. Most responding dentists considered the chair-side CAD/CAM system as important in terms of time saving, boosting the number of visiting patients and income improvement. The willingness to dedicate the time and effort to learn the chair-side CAD/CAM technology was apparent amid a high proportion of dentists (75.4%). The findings of this pilot survey reflect broad satisfaction and positive attitude among the surveyed dentists towards use and outcome of chair-side CAD/CAM technology in the dental clinical practice. It seems that the CAD/CAM technology has infiltrated into the workflow of Saudi dental practices with speculations of growing implementation among the wider sector of dental practitioners in the future.
Quantitative Assessment of Impact and Sensitivity of Imaging Spectroscopy for Monitoring of Ageing of Archival Documents
Authors: Padoan Roberto , Klein Marvin E. , Groves Roger M. , de Bruin Gerrit , Steemers Ted A. G. , Strlič Matija
Published: January 2021

Ageing of historical documents often results in changes in the optical properties of the constituent materials. Imaging spectroscopy (IS) can be a valuable tool for monitoring of such changes, if the method fulfils two important conditions. Firstly, compared to natural ageing, the accumulated light dose from repeated measurements of the monitored document must not induce any significant degradation. Secondly, the monitoring instrumentation and procedures should be sensitive enough to detect changes in the materials before they become visible. We present experimental methods to evaluate the suitability of IS instrumentation for monitoring purposes. In the first set of experiments, the impact of repeated monitoring measurements was determined using a set of Blue Wool Standard materials. In the second set of experiments, the capability of the instrument to detect spectral changes was tested using ISO standard materials and several documents representative of European archive collections. It is concluded that the tested hyperspectral instrument is suitable for monitoring of the colour change of documents during display. The described experimental approach can be recommended to test the suitability of other imaging spectroscopy instruments for monitoring applications.