bluuebunny/crossref_metadata_2025 huggingface dataset:
Survey of National Health Service (NHS) orthodontic practitioners in Wales, UK. Part 1: working patterns 2021–2022
Authors: Lewis Benjamin RK , Spicer Olivia J , McLaughlin William S , Moons Kirstie
Published: January 2024

Objective:

To ascertain the working patterns of the NHS orthodontic workforce in Wales and any possible future changes.

Design:

Descriptive cross-sectional survey.

Participants:

NHS orthodontic practitioners in Wales.

Methods:

An anonymised email distributed an electronic two-part survey of the Welsh NHS orthodontic workforce. The survey consisted of three sections: (1) demographic information; (2) respondents’ working pattern (part 1); and (3) perceptions of professional satisfaction (part 2).

Results:

Part 1 of the survey yielded a 70.5% response rate (n = 79); 65.8% of the respondents were women. Of the respondents, 45.6% (n = 36) worked full time (F/T), 39.2% (n = 31) worked less than F/T and 15.2% (n = 12) worked more than F/T. Of the male respondents, 81.5% (n = 22) worked 10 sessions or more compared to 50% (n = 26) of women. The respondents undertook 508.5 orthodontic clinical sessions per week within Wales; of these sessions, 87.6% (n = 445.5) delivered NHS orthodontic care. Of the respondents, 8.4% (n = 7) were planning to increase their orthodontic clinical time within the next 2 years, 24.1% (n = 19) were planning to decrease it and 20.3% (n = 16) were unsure. One-quarter of respondents indicated that they were planning to stop clinical orthodontic activity within the next 5 years, including 53.3% (n = 8) of DwSIs, 37% (n = 10) of primary care specialists and 13.3% (n = 2) of consultants. The pandemic was an influencing factor for 80% of these clinicians.

Conclusions:

Part 1 of the survey suggested that the majority of the orthodontic workforce was female, were working full time or more, and spent most sessions delivering NHS care. One-quarter of respondents were planning to cease undertaking orthodontic activity within the next 5 years.
Healthcare Workers’ Perceptions of Work-Related Stress and Burnout: Strategies and Barriers for Self-Care
Authors: Kober Michelle , Chang Yu-Ping
Published: January 2024

Background: There is little research providing critical understanding of how healthcare professionals perceive and manage work-related stress. This study aims to understand healthcare workers’ perspectives regarding work-related stress and burnout, strategies and barriers for self-care, and organizational support for self-care and resiliency. Methods: A qualitative descriptive approach was used. Individual, semi-structured interviews were conducted with healthcare workers from 5 organizations in New York State. Data was analyzed using Braun and Clarke’s Thematic Analysis. Results: Interviews were conducted with 27 healthcare workers from various disciplines. Findings indicated that healthcare workers experienced high levels of stress and burnout, which negatively impacts their mental health, quality of work, relationships with coworkers, and patient care. Many participants expressed an interest in self-care activities, and there were apparent differences among respondents regarding perceived organizational support. Five themes were identified, including: staff shortages, coworker conflict and interactions, strategies to mitigate stress, impacts of work-related stress, and managing stress and burnout in the workplace. Conclusion: Workplace stress affects patient care, but workers feel that there is limited support from leadership. It is critical that healthcare organizations and governments prioritize providing support along with other resources to healthcare workers to address burnout and assist with mental health concerns.
Prospective single-center study on the reliability of ipsilateral cerebral oximetry using near-infrared spectroscopy as a predictor for selective shunting during carotid endarterectomy
Authors: Alhaizaey Abdullah , Yousif Mohamed , Azazy Ahmed , Saber Abdelgawad , Safan Mohamed , Elgamal Galal A , Almalki Yahya , Alhazmi Barrag
Published: January 2024

Objective

Many techniques are available for the intraoperative assessment of brain perfusion during carotid endarterectomy, such as carotid stump pressure, near-infrared spectroscopy, somatosensory evoked potentials, transcranial Doppler, electroencephalography, and clinical assessment. The decision for selective carotid shunt insertion is dependent on clinical deterioration or the detection of cerebral hypoperfusion after cross-clamping of the internal carotid artery. Monitoring cerebral oximetry using near-infrared spectroscopy is a noninvasive technique for cerebral oxygen saturation measurement, reflecting changes in cerebral blood flow during carotid endarterectomy. The aim of this study was to evaluate the reliability of near-infrared spectroscopy as a predictor of selective shunting during carotid endarterectomy.

Methods

In total, 47 conventional carotid endarterectomy surgeries were performed at our hospital between March 2016 and December 2021. All surgeries were performed under a regional cervical block supplemented with local infiltration anesthesia. All patients were monitored by cerebral oximetry using bilateral near-infrared spectroscopy probes and clinical assessment through communication with the patient (numerical, visual, and verbal) to indicate a selective shunt. Near-infrared spectroscopy values were recorded before and after internal carotid cross-clamping and after declamping. Any decrease in ipsilateral cerebral oximetry-near-infrared spectroscopy values equal to or more than 20% from the pre-clamping baseline reading associated with deterioration in neurological status (hemiparesis, aphasia, or deterioration in level of consciousness) after internal carotid artery cross-clamping was considered an indication for intraluminal carotid shunting

Results

After internal carotid artery cross-clamping, 5 of 47 patients (10.6%) developed a significant drop in cerebral oxygen saturation associated with obvious clinical assessment deterioration in verbal communication and weakness in contralateral arm power. A Pruitt-Inahara carotid shunt was subsequently inserted, and 42 patients remained stable throughout surgery. The average decline in ipsilateral near-infrared spectroscopy values was 23.8% in patients with clinical deterioration. The average decline was 8.6% in patients who remained stable.

Conclusions

Monitoring ipsilateral cerebral oximetry using near-infrared spectroscopy is an easy and reliable method for indicating selective shunting during carotid endarterectomy. A 20% decrease in ipsilateral brain tissue oximetry after internal carotid artery cross-clamping provides a reliable cut-off value for selective intraluminal carotid shunting during carotid endarterectomy.
Measuring uterine cavity volume with sonohysterography: A new objective method
Authors: Elsayed Mortada , Amer Mohamed I , Gamal Rania , Haggag Aly , Mashaly Ahmed R , Absi Tamer El , Elsayed Walaa
Published: January 2024

Introduction:

The uterine cavity is a potential space with limited methods for evaluating its volume, limiting the evaluation of interventions’ effectiveness in various uterine conditions. This study aims to objectively measure the uterine cavity volume using sonohysterography coupled with a Foley catheter to provide a normative model of age and parity-related uterine cavity volume.

Methods:

The research included 35 women (group 1) with a total abdominal hysterectomy and 150 women (group 2) who underwent sonohysterography for various gynecologic indications. Saline infusion sonography was administered to all patients. The most common shape obtained after the saline infusion was taken to measure the uterine cavity’s dimensions and volume. The uterine cavity volumes, as measured by sonohysterography, and the volumes of saline injected after the hysterectomy were compared.

Results:

A significant association exists between uterine cavity volumes measured by sonohysterography and true volumes measured immediately after hysterectomy ( p = 0.001). The association between uterine cavity volume measured by sonohysterography and using only a Foley catheter balloon was statistically insignificant ( p = 0.13). A statistically significant positive association was observed between the uterine cavity volume and the patient’s age and parity ( p ⩽ 0.05).

Conclusion:

Measuring the uterine cavity volume using a paediatric Foley catheter balloon coupled with sonohysterography offers an objective approach to measuring a normal (without gross pathologies) uterus volume. This technique would improve the diagnostic accuracy and the management of women with distinct uterine cavity morphologies.
Gender-based differential management of acute low back pain in the emergency department: A survey based on a clinical vignette
Authors: Schilter Léa V , Le Boudec Joana AE , Hugli Olivier , Locatelli Isabella , Staeger Phillippe , Della Santa Vincent , Frochaux Vincent , Rutschmann Olivier , Bieler Sandra , Ribordy Vincent , Fournier Yvan , Decosterd Dumeng , Clair Carole
Published: January 2024

Background:

Women may receive suboptimal pain management compared with men, and this disparity might be related to gender stereotypes.

Objectives:

To assess the influence of patient gender on the management of acute low back pain.

Design:

We assessed pain management by 231 physicians using an online clinical vignette describing a consultation for acute low back pain in a female or male patient. The vignette was followed by a questionnaire that assessed physicians’ management decisions and their gender stereotypes.

Methods:

We created an online clinical vignette presenting a patient with acute low back pain and assessed the influence of a patient’s gender on pain management. We investigated gender-related stereotyping regarding pain care by emergency physicians using the Gender Role Expectation of Pain questionnaire.

Results:

Both male and female physicians tended to consider that a typical man was more sensitive to pain, had less pain endurance, and was more willing to report pain than a typical woman. These stereotypes did not translate into significant differences in pain management between men and women. However, women tended to be referred less often for imaging examinations than men and were also prescribed lower doses of ibuprofen and opioids. The physician’s gender had a modest influence on management decisions, female physicians being more likely to prescribe ancillary examinations.

Conclusion:

We observed gender stereotypes among physicians. Our findings support the hypothesis that social characteristics attributed to men and women influence pain management. Prospective clinical studies are needed to provide a deeper understanding of gender stereotypes and their impact on clinical management.
Common maternal health problems and their correlates in early post-partum mothers
Authors: Ng Christine , Szücs Anna , Goh Lay Hoon
Published: January 2024

Background:

Mothers experience a wide range of maternal health problems after childbirth, which in turn, affect their well-being and ability to care for their newborn. These problems may be influenced by factors such as mode of delivery or socio-economic status.

Objective:

This study aims to investigate the prevalence of common maternal health problems and their correlates in a public primary healthcare institution in Singapore.

Design:

This was a cross-sectional study, based on a self-administered questionnaire.

Methods:

A total of 373 mothers (mean age 31.9 years) who accompanied their infants for their 4- to 8-week development assessments at a public primary care clinic in Singapore completed a self-administered questionnaire from June 2021 to December 2021. The questionnaire assessed demographic factors, mode of delivery, number of children, number of individuals providing significant help, and the frequency of common physical and mental maternal health problems using a 5-point Likert-type scale.

Results:

The five most common maternal health problems were fatigue (77.7%), lower back pain (59.3%), Caesarean wound pain (54.3%), upper back pain (53.0%) and vaginal pain (41.2%). The prevalence of depression and anxiety was 22.0% and 11.3%, respectively. With respect to the symptoms’ correlates, pain on passing urine was more frequent after assisted vaginal deliveries than all other forms of deliveries (all pairwise p < 0.01), and pain on passing motion was more frequent in vaginal deliveries than in Caesarean deliveries (all pairwise p < 0.05). Mothers having a larger number of children more frequently experienced headaches (β = 0.17, SE = 0.05, p = 0.002) and less frequently experienced breastfeeding difficulties (β = –0.28, SE = 0.08, p < 0.001).

Conclusion:

Mothers experienced a high prevalence of maternal health problems in multiple domains during the first 8 weeks after childbirth. Mode of delivery and number of children were associated with increased prevalence of certain problems. Mothers’ physical and mental well-being should be investigated early after delivery and addressed with adequate treatments and resources.
Motivation Science Can Improve Diversity, Equity, and Inclusion (DEI) Trainings
Authors: Legate Nicole , Weinstein Netta
Published: January 2024

Recent reviews of efforts to reduce prejudice and increase diversity, equity, and inclusion (DEI) in the workplace have converged on the conclusion that prejudice is resistant to change and that merely raising awareness of the problem is not enough. There is growing recognition that DEI efforts may fall short because they do not effectively motivate attitudinal and behavioral change, especially the type of change that translates to reducing disparities. Lasting change requires sustained effort and commitment, yet insights from motivation science about how to inspire this are missing from the scientific and practitioner literatures on DEI trainings. Herein, we leverage evidence from two complementary approaches to motivating change and reducing defensiveness: self-determination theory, a metatheory of human motivation, and motivational interviewing, a clinical approach for behavior change, to tackle the question of how to improve DEI efforts. We distill these insights for researchers, teachers, practitioners, and leaders wanting to apply motivational principles to their own DEI work. We highlight challenges of using this approach and recommend training takes place alongside larger structural and organizational changes. We conclude that motivation is a necessary (but insufficient) ingredient for effective DEI efforts that can energize personal commitment to DEI.
The predictability of court-adjudicated compensation for pain and suffering damages within the criminal proceedings and the role of victim labels: A case study on victims of sexual crime in the Netherlands
Authors: Augusteijn Freya , Pemberton Antony , CJH Bijleveld Catrien , Bosma Alice K
Published: January 2024

Compensation for pain and suffering damages has been a focal point within legal academic debate. The difficulty of placing a monetary value on such damages and the lack of well-defined guidelines have been claimed to result in widely varying compensatory awards, making them random and unpredictable. Variability may arise from victim labels depicting who is deserving, and who is not. In this study, regression analysis is employed to assess whether compensation can be predicted, and what role victim labels play in the judicial decision-making. The empirical strategy is informed by notion of the ideal victim. Results show that compensation for pain and suffering damages can be predicted, and that by including victim labels the prediction significantly improves. Although they are not the predominant predictors, victim labels of blamelessness play a role in awarded compensation. The main driver in judges’ decision is the claim put forward by the victim.
Binge-watching and mental illness versus comfort TV and mental health in WandaVision
Authors: Moore Christopher L , Comerford Chris , Vettoretto Ren
Published: January 2024

WandaVision launched the Disney+ subscription video-on-demand (SVOD) platform by blending the sitcom and the superhero genres in a nostalgia-inducing fusion of Marvel comics, cinema and television. The series represents the canonisation of Marvel media into a single cohesive narrative ‘multiverse’, yet the story focuses on the personal experience of the character, Wanda, and her struggle with loss, grief, and Post-Traumatic Stress Disorder (PTSD). In this article, we explore how WandaVision presents a unique examination of the tension between the role of screen media as comfort TV and the pervasive fears over the obsessive escapism of binge-watching. The article also assesses the potential risks of excessive media consumption through the framing of American sitcoms and superhero families from the 1950s and onward.
The role of aberrant DNA methylation in cancer initiation and clinical impacts
Authors: Geissler Franziska , Nesic Ksenija , Kondrashova Olga , Dobrovic Alexander , Swisher Elizabeth M. , Scott Clare L. , J. Wakefield Matthew
Published: January 2024

Epigenetic alterations, including aberrant DNA methylation, are now recognized as bone fide hallmarks of cancer, which can contribute to cancer initiation, progression, therapy responses and therapy resistance. Methylation of gene promoters can have a range of impacts on cancer risk, clinical stratification and therapeutic outcomes. We provide several important examples of genes, which can be silenced or activated by promoter methylation and highlight their clinical implications. These include the mismatch DNA repair genes MLH1 and MSH2, homologous recombination DNA repair genes BRCA1 and RAD51C, the TERT oncogene and genes within the P15/P16/RB1/E2F tumour suppressor axis. We also discuss how these methylation changes might occur in the first place – whether in the context of the CpG island methylator phenotype or constitutional DNA methylation. The choice of assay used to measure methylation can have a significant impact on interpretation of methylation states, and some examples where this can influence clinical decision-making are presented. Aberrant DNA methylation patterns in circulating tumour DNA (ctDNA) are also showing great promise in the context of non-invasive cancer detection and monitoring using liquid biopsies; however, caution must be taken in interpreting these results in cases where constitutional methylation may be present. Thus, this review aims to provide researchers and clinicians with a comprehensive summary of this broad, but important subject, illustrating the potentials and pitfalls of assessing aberrant DNA methylation in cancer.
Same Words, Different Meanings—Same Courts, Different Leanings: How the Supreme Court’s Latest Religious Accommodation Holding Changes the Law and Affects Employers
Authors: Lakhani Tashlin , Sherwyn David , Wagner Paul
Published: January 2024

The Supreme Court dropped several of its most anxiously awaited and controversial cases during the last week of June 2023. While two of the cases, 303 Creative LLC v. Elenis and Students for Fair Admissions., Inc., v. President & Fellows of Harvard College, received most of the press, a third case will likely be the most consequential of the three for the hospitality industry. In Groff v. DeJoy, Postmaster General, the Supreme Court, in a 9-0 decision, rejected a 25 year+ interpretation of a Supreme Court case defining employers’ obligations to accommodate religion. Because the Court released its Groff decision during the same week as the release of both 303 Creative and the Harvard cases, Groff was lost in the shuffle. However, we argue that Groff’s imprecise but radical change of what constitutes an undue hardship for religious accommodations under Title VII of the Civil Rights Act of 1964 will create confusion, may cause dissention, and will add to an already difficult labor market in hospitality and other industries. To support our proposition, this article examines (a) the development of religious accommodation law before 1977, (b) the 1977 Supreme Court case that the Groff Court rejected, (c) the subsequent precedent of that 1977 case, (d) the passing and development of the Americans with Disabilities Act, and then, (e) the effect of Groff.
Exploring Duoethnography in ELT Research Ecosystems: Accessibility, Misuse, and Further Horizons
Authors: Lowe Robert J. , Jones Marc
Published: January 2024

Duoethnography is an approach to qualitative research in which two researchers engage in dialogue to understand how a particular phenomenon has manifested differently in their lives, and in so doing, aim to come to a new understanding of that phenomenon. In this paper, two applied linguists from different research backgrounds, and with different priorities, engage in a metaduoethnography to explore what they each find of value of the method for their separate research programs. Through dialogue, they uncover what first drew each of them to duoethnography, and go on to both praise and problematize the accessibility of the method. Following this, they use the concept of the “research ecosystem” to investigate the role duoethnography plays, or may play, in their separate and evolving research programs, particularly focusing on the concepts of reflexivity and praxis. It is hoped that this exploratory dialogue may resonate with other researchers, and assist them in evaluating the benefits of duoethnography for their work.
Changes in Sports Participation, Specialization, and Burnout From 7th to 12th Grade: Final Results From a 6-Year Longitudinal Study
Authors: Valenzuela-Moss Jacquelyn , Sini Milo , Wren Tishya A.L. , Edison Bianca R.
Published: January 2024

Background:

Previous studies show alarming rates of burnout and dropout from sports participation and physical activity in the youth population. Early sports specialization may increase the risk of injury, burnout, and eventual dropout from sports.

Hypothesis:

Sports participation will decrease, specialization will increase, and burnout will increase from junior high to high school.

Study Design:

Prospective longitudinal study.

Level of Evidence:

Level 2b.

Methods:

The graduating class of 2023 was surveyed longitudinally in the fall of each school year from 7th to 12th grade (n = 35-77 from middle to high school based on new enrollment participants and attrition). Survey questions asked about sports participation, specialization, and burnout. Responses were analyzed by grade level using mixed effects linear and logistic regression accounting for repeated measures.

Results:

The number of days per week in which students engaged in at least 60 minutes of strenuous exercise progressively decreased from 7th through 12th grade from 4.0 to 2.3 days per week ( P < 0.01). The percentage of students participating in sports also decreased from 7th to 12th grade from 82% to 39% ( P < 0.01). More students stopped participating in a sport (38% vs 22% to 29%, P = 0.04), quit a sport to focus on a single sport (60% vs 46% to 49%, P = 0.01), or reported that 1 sport was most important to them (71% vs 54% to 67%, P = 0.06) in 9th grade. Burnout in sports did not differ significantly by grade level (19% to 23%, P > 0.99). In contrast, burnout in school was significantly more common in high school (54% to 69%) compared with middle school (36%, P < 0.01). Burnout in school was higher in female students compared with male students (68% vs 38%, P < 0.01).

Conclusion:

Organized sports participation and general physical activity decreased from middle to high school, with the entry into high school at 9th grade being a time at which a significant number of students dropped other sports to specialize in a single sport or stopped participating. Burnout in sports did not appear to increase with age; however, there was an increase in school-related burnout as students transitioned from middle to high school.

Clinical Relevance:

Our results highlight the need for continued research into sports participation, specialization, and burnout in adolescents. In addition, health professionals and the community (parents, coaches, teachers) supporting youth athletes may consider positive interventions during the periods of transition from middle to high school to include facilitating different tracks of sports participation (including a developmental or recreational model of play) to reduce dropout from sport and supportive measures to lessen burnout from sports and from school.
Subchondral Bone Alignment in Osteochondral Allograft Transplants for Large Oval Defects of the Medial Femoral Condyle: Comparison of Lateral versus Medial Femoral Condyle Donors
Authors: Taylor Kelly M.R. , Locke Conor S. , Mologne Timothy S. , Bugbee William D. , Grant John A.
Published: January 2024

Objective

Supply-demand mismatch of medial femoral condyle (MFC) osteochondral allografts (OCAs) remains a rate-limiting factor in the treatment of osteochondral defects of the femoral condyle. Surface contour mapping was used to determine whether a contralateral lateral femoral condyle (LFC) versus ipsilateral MFC OCA differs in the alignment of donor:native subchondral bone for large osteochondral defects of the MFC.

Design

Thirty fresh-frozen human femoral condyles were matched by tibial width into 10 groups of 3 condyles (MFC recipient, MFC donor, and LFC donor) each for 3 cartilage surgeons (90 condyles). The recipient MFC was imaged using nano-computed tomography scan. Donor oval grafts were harvested from each matched condyle and transplanted into a 17 mm × 36 mm defect created in the recipient condyle. Following the first transplant, the recipient condyle was imaged and superimposed on the native condyle nano-CT scan. The donor plug was removed and the process repeated for the other donor. Surface height deviation and circumferential step-off height deviation were compared between native and donor subchondral bone surfaces for each transplant.

Results

There was no statistically significant difference in mean subchondral bone surface deviation (LFC = 0.87 mm, MFC = 0.76 mm, P = 0.07) nor circumferential step-off height (LFC = 0.93 mm, MFC = 0.85 mm, P = 0.09) between the LFC and MFC plugs. There were no significant differences in outcomes between surgeons.

Conclusions

There were no significant differences in subchondral bone circumferential step-off or surface deviation between ipsilateral MFC and contralateral LFC oval-shaped OCAs for 17 mm × 36 mm defects of the MFC.
Meconium peritonitis in multiple intestinal atresia with combined immune deficiency caused by a TTC7A mutation: A case report
Authors: Chen Jiongfei , Chen Meiwen , Lin Ya , Li Xiangjuan
Published: January 2024

Multiple intestinal atresia with combined immune deficiency is a severe autosomal recessive disorder caused by the tetratricopeptide repeat domain 7A ( TTC7A) gene deficiency, which is characterized by extensive intestinal defects with immune deficiency. This report describes a fetus with TTC7A deficiency who developed meconium peritonitis in utero. Evidence suggests that patients with TTC7A deficiency present with intestinal defects as early as in utero. In this case, intestinal abnormalities were considered during the prenatal examination at week 28, and chromosome and genetic tests were performed. The results indicated that the fetus had a TTC7A complex heterozygous mutation. The male infant underwent surgical treatment after birth and developed severe infection and sepsis, which confirmed the presence of multiple intestinal atresia with combined immune deficiency. Our case suggests an association between meconium peritonitis and the TTC7A gene deficiency, indicating the possibility of severe intestinal defects and immune deficiencies after birth and guiding subsequent fetal treatment choices.
What is public trust in national electronic health record systems? A scoping review of qualitative research studies from 1995 to 2021
Authors: Papadopoulos Kimon , von Wyl Viktor , Gille Felix
Published: January 2024

Objective

Public trust in national electronic health record systems is essential for the successful implementation within a healthcare system. Research investigating public trust in electronic health records is limited, leading to a lack of conceptual clarity. In response, the objective of this study is to gain a clearer understanding on the conceptualizations of public trust in electronic health records, which can support the implementation of national electronic health record systems.

Methods

Guided by the PRISMA-ScR checklist, a scoping review of 27 qualitative studies on public trust in electronic health records found between January 2022 and June 2022 was conducted using an inclusive search method. In an iterative process, conceptual themes were derived describing the promoters and outcomes of public trust in electronic health records.

Results

Five major conceptual themes with 15 sub-themes were present across the literature. Comprehension, autonomy, and data protection promote public trust in electronic health record; while personal and system benefits are the outcomes once public trust in electronic health records exists. Additional findings highlight the pivotal role of healthcare actors for the public trust building process.

Conclusions

The results underscore comprehension, autonomy, and data protection as important themes that help ascertain and solidify public trust in electronic health records. As well, health system actors have the capacity to promote or hinder national electronic health record implementation, depending on their actions and how the public perceives those actions. The findings can assist researchers, policymakers, and other health system actors in attaining a better understanding of the intricacies of public trust in electronic health records.
After a Decade of Decline, the US Undocumented Population Increased by 650,000 in 2022
Authors: Warren Robert
Published: January 2024

Executive Summary

This report describes estimates of the undocumented population residing in the United States in 2022 compiled by the Center for Migration Studies of New York (CMS). The estimates are based on data collected in the American Community Survey (ACS) conducted by the US Census Bureau ( Ruggles et al. 2023 ). The report finds that the undocumented population grew from 10.3 million in 2021 to 10.9 million in 2022, an increase of 650,000. The increase reverses more than a decade of gradual decline. The undocumented populations from 10 countries increased by a total of 525,000: Mexico, the Dominican Republic, and India; El Salvador, Guatemala, and Honduras in Central America; and Brazil, Colombia, Ecuador, and Venezuela in South America. The undocumented population in Florida increased by about 125,000 in 2022, Texas increased by 60,000, New York by 50,000, and Maryland by 45,000. The report explains why undocumented population growth is much less than the number of apprehensions by DHS. Finally, the Appendix provides a detailed description of the CMS methodology.
Resource Utilization and Costs Associated with Approaches to Identify Infants with Early-Onset Sepsis
Authors: Guan Grace , Joshi Neha S. , Frymoyer Adam , Achepohl Grace D. , Dang Rebecca , Taylor N. Kenji , Salomon Joshua A. , Goldhaber-Fiebert Jeremy D. , Owens Douglas K.
Published: January 2024

Objective. To compare resource utilization and costs associated with 3 alternative screening approaches to identify early-onset sepsis (EOS) in infants born at ≥35 wk of gestational age, as recommended by the American Academy of Pediatrics (AAP) in 2018. Study Design. Decision tree–based cost analysis of the 3 AAP-recommended approaches: 1) categorical risk assessment (categorization by chorioamnionitis exposure status), 2) neonatal sepsis calculator (a multivariate prediction model based on perinatal risk factors), and 3) enhanced clinical observation (assessment based on serial clinical examinations). We evaluated resource utilization and direct costs (2022 US dollars) to the health system. Results. Categorical risk assessment led to the greatest neonatal intensive care unit usage (210 d per 1,000 live births) and antibiotic exposure (6.8%) compared with the neonatal sepsis calculator (112 d per 1,000 live births and 3.6%) and enhanced clinical observation (99 d per 1,000 live births and 3.1%). While the per-live birth hospital costs of the 3 approaches were similar—categorical risk assessment cost $1,360, the neonatal sepsis calculator cost $1,317, and enhanced clinical observation cost $1,310—the cost of infants receiving intervention under categorical risk assessment was approximately twice that of the other 2 strategies. Results were robust to variations in data parameters. Conclusion. The neonatal sepsis calculator and enhanced clinical observation approaches may be preferred to categorical risk assessment as they reduce the number of infants receiving intervention and thus antibiotic exposure and associated costs. All 3 approaches have similar costs over all live births, and prior literature has indicated similar health outcomes. Inclusion of downstream effects of antibiotic exposure in the neonatal period should be evaluated within a cost-effectiveness analysis.

Highlights

Of the 3 approaches recommended by the American Academy of Pediatrics in 2018 to identify early-onset sepsis in infants born at ≥35 weeks, the categorical risk assessment approach leads to about twice as many infants receiving evaluation to rule out early-onset sepsis compared with the neonatal sepsis calculator and enhanced clinical observation approaches. While the hospital costs of the 3 approaches were similar over the entire population of live births, the neonatal sepsis calculator and enhanced clinical observation approaches reduce antibiotic exposure, neonatal intensive care unit admission, and hospital costs associated with interventions as part of the screening approach compared with the categorical risk assessment approach.
A Delphi Study to Strengthen Research-Methods Training in Undergraduate Psychology Programs
Authors: Thibault Robert T. , Bailey-Rodriguez Deborah , Bartlett James E. , Blazey Paul , Green Robin J. , Pownall Madeleine , Munafò Marcus R.
Published: January 2024

Psychology programs often emphasize inferential statistical tests over a solid understanding of data and research design. This imbalance may leave graduates underequipped to effectively interpret research and employ data to answer questions. We conducted a two-round modified Delphi to identify the research-methods skills that the UK psychology community deems essential for undergraduates to learn. Participants included 103 research-methods instructors, academics, students, and nonacademic psychologists. Of 78 items included in the consensus process, 34 reached consensus. We coupled these results with a qualitative analysis of 707 open-ended text responses to develop nine recommendations for organizations that accredit undergraduate psychology programs—such as the British Psychological Society. We recommend that accreditation standards emphasize (1) data skills, (2) research design, (3) descriptive statistics, (4) critical analysis, (5) qualitative methods, and (6) both parameter estimation and significance testing; as well as (7) give precedence to foundational skills, (8) promote transferable skills, and (9) create space in curricula to enable these recommendations. Our data and findings can inform modernized accreditation standards to include clearly defined, assessable, and widely encouraged skills that foster a competent graduate body for the contemporary world.
Total variation denoising-based method of identifying the states of single molecules in break junction data
Authors: Komoto Yuki , Ryu Jiho , Taniguchi Masateru
Published: January 2024

Break junction (BJ) measurements provide insights into the electrical properties of diverse molecules, enabling the direct assessment of single-molecule conductances. The BJ method displays potential for use in determining the dynamics of individual molecules, single-molecule chemical reactions, and biomolecules, such as deoxyribonucleic acid and ribonucleic acid. However, conductance data obtained via single-molecule measurements may be susceptible to fluctuations due to minute structural changes within the junctions. Consequently, clearly identifying the conduction states of these molecules is challenging. This study aims to develop a method of precisely identifying conduction state traces. We propose a novel single-molecule analysis approach that employs total variation denoising (TVD) in signal processing, focusing on the integration of information technology with measured single-molecule data. We successfully applied this method to simulated conductance traces, effectively denoise the data, and elucidate multiple conduction states. The proposed method facilitates the identification of well-defined plateau lengths and supervised machine learning with enhanced accuracies. The introduced TVD-based analytical method is effective in elucidating the states within the measured single-molecule data. This approach exhibits the potential to offer novel perspectives regarding the formation of molecular junctions, conformational changes, and cleavage.
Flux sampling in genome-scale metabolic modeling of microbial communities
Authors: Gelbach Patrick E. , Cetin Handan , Finley Stacey D.
Published: January 2024

Background

Microbial communities play a crucial role in ecosystem function through metabolic interactions. Genome-scale modeling is a promising method to understand these interactions and identify strategies to optimize the community. Flux balance analysis (FBA) is most often used to predict the flux through all reactions in a genome-scale model; however, the fluxes predicted by FBA depend on a user-defined cellular objective. Flux sampling is an alternative to FBA, as it provides the range of fluxes possible within a microbial community. Furthermore, flux sampling can capture additional heterogeneity across a population, especially when cells exhibit sub-maximal growth rates.

Results

In this study, we simulate the metabolism of microbial communities and compare the metabolic characteristics found with FBA and flux sampling. With sampling, we find significant differences in the predicted metabolism, including an increase in cooperative interactions and pathway-specific changes in predicted flux.

Conclusions

Our results suggest the importance of sampling-based approaches to evaluate metabolic interactions. Furthermore, we emphasize the utility of flux sampling in quantitatively studying interactions between cells and organisms.
Predicting lncRNA-disease associations using multiple metapaths in hierarchical graph attention networks
Authors: Yao Dengju , Deng Yuexiao , Zhan Xiaojuan , Zhan Xiaorong
Published: January 2024

Background

Many biological studies have shown that lncRNAs regulate the expression of epigenetically related genes. The study of lncRNAs has helped to deepen our understanding of the pathogenesis of complex diseases at the molecular level. Due to the large number of lncRNAs and the complex and time-consuming nature of biological experiments, applying computer techniques to predict potential lncRNA-disease associations is very effective. To explore information between complex network structures, existing methods rely mainly on lncRNA and disease information. Metapaths have been applied to network models as an effective method for exploring information in heterogeneous graphs. However, existing methods are dominated by lncRNAs or disease nodes and tend to ignore the paths provided by intermediate nodes.

Methods

We propose a deep learning model based on hierarchical graphical attention networks to predict unknown lncRNA-disease associations using multiple types of metapaths to extract features. We have named this model the MMHGAN. First, the model constructs a lncRNA-disease–miRNA heterogeneous graph based on known associations and two homogeneous graphs of lncRNAs and diseases. Second, for homogeneous graphs, the features of neighboring nodes are aggregated using a multihead attention mechanism. Third, for the heterogeneous graph, metapaths of different intermediate nodes are selected to construct subgraphs, and the importance of different types of metapaths is calculated and aggregated to obtain the final embedded features. Finally, the features are reconstructed using a fully connected layer to obtain the prediction results.

Results

We used a fivefold cross-validation method and obtained an average AUC value of 96.07% and an average AUPR value of 93.23%. Additionally, ablation experiments demonstrated the role of homogeneous graphs and different intermediate node path weights. In addition, we studied lung cancer, esophageal carcinoma, and breast cancer. Among the 15 lncRNAs associated with these diseases, 15, 12, and 14 lncRNAs were validated by the lncRNA Disease Database and the Lnc2Cancer Database, respectively.

Conclusion

We compared the MMHGAN model with six existing models with better performance, and the case study demonstrated that the model was effective in predicting the correlation between potential lncRNAs and diseases.
Comparative analysis and characterization of the chloroplast genome of Krascheninnikovia ceratoides (Amarathaceae): a xerophytic semi-shrub exhibiting drought resistance and high-quality traits
Authors: Liu Yuping , Zheng Changyuan , Su Xu , Chen Jinyuan , Li Xiaoli , Sun Chenglin , Nizamani Mir Muhammad
Published: January 2024

Background

Krascheninnikovia ceratoides, a perennial halophytic semi-shrub belonging to the genus Krascheninnikovia (Amarathaceae), possesses noteworthy ecological, nutritional, and economic relevance. This species is primarily distributed across arid, semi-arid, and saline-alkaline regions of the Eurasian continent, encompassing Inner Mongolia, Xinjiang, Qinghai, Gansu, Ningxia, and Tibet.

Results

We reported the comprehensive chloroplast (cp) genome of K. ceratoides, characterized by a circular conformation spanning 151,968 bp with a GC content of 36.60%. The cp genome encompassed a large single copy (LSC, 84,029 bp), a small single copy (SSC, 19,043 bp), and a pair of inverted repeats (IRs) regions (24,448 bp each). This genome harbored 128 genes and encompassed 150 simple sequence repeats (SSRs). Through comparative analyses involving cp genomes from other Cyclolobeae (Amarathaceae) taxa, we observed that the K. ceratoides cp genome exhibited high conservation, with minor divergence events in protein-coding genes (PCGs) accD, matK, ndhF, ndhK, ycf1, and ycf2. Phylogenetic reconstructions delineated K. ceratoides as the sister taxon to Atriplex, Chenopodium, Dysphania, and Suaeda, thus constituting a robust clade. Intriguingly, nucleotide substitution ratios (Ka/Ks) between K. ceratoides and Dysphania species for ycf1 and ycf2 genes surpassed 1.0, indicating the presence of positive selection pressure on these loci.

Conclusions

The findings of this study augment the genomic repository for the Amarathaceae family and furnish crucial molecular instruments for subsequent investigations into the ecological adaptation mechanisms of K. ceratoides within desert ecosystems.
Single-cell RNA-seq mapping of chicken peripheral blood leukocytes
Authors: Maxwell Matilda , Söderlund Robert , Härtle Sonja , Wattrang Eva
Published: January 2024

Background

Single-cell transcriptomics provides means to study cell populations at the level of individual cells. In leukocyte biology this approach could potentially aid the identification of subpopulations and functions without the need to develop species-specific reagents. The present study aimed to evaluate single-cell RNA-seq as a tool for identification of chicken peripheral blood leukocytes. For this purpose, purified and thrombocyte depleted leukocytes from 4 clinically healthy hens were subjected to single-cell 3′ RNA-seq. Bioinformatic analysis of data comprised unsupervised clustering of the cells, and annotation of clusters based on expression profiles. Immunofluorescence phenotyping of the cell preparations used was also performed.

Results

Computational analysis identified 31 initial cell clusters and based on expression of defined marker genes 28 cluster were identified as comprising mainly B-cells, T-cells, monocytes, thrombocytes and red blood cells. Of the remaining clusters, two were putatively identified as basophils and eosinophils, and one as proliferating cells of mixed origin. In depth analysis on gene expression profiles within and between the initial cell clusters allowed further identification of cell identity and possible functions for some of them. For example, analysis of the group of monocyte clusters revealed subclusters comprising heterophils, as well as putative monocyte subtypes. Also, novel aspects of TCRγ/δ + T-cell subpopulations could be inferred such as evidence of at least two subtypes based on e.g., different expression of transcription factors MAF, SOX13 and GATA3. Moreover, a novel subpopulation of chicken peripheral B-cells with high SOX5 expression was identified. An overall good correlation between mRNA and cell surface phenotypic cell identification was shown.

Conclusions

Taken together, we were able to identify and infer functional aspects of both previously well known as well as novel chicken leukocyte populations although some cell types. e.g., T-cell subtypes, proved more challenging to decipher. Although this methodology to some extent is limited by incomplete annotation of the chicken genome, it definitively has benefits in chicken immunology by expanding the options to distinguish identity and functions of immune cells also without access to species specific reagents.
Atopic dermatitis pediatric patients show high rates of nasal and intestinal colonization by methicillin-resistant Staphylococcus aureus and coagulase-negative staphylococci
Authors: Augusto de Oliveira Mariana Fernandes , Agne Daiane Bitencourt , Bastos Ludmila Sento Sé , Andrade de Oliveira Laura Maria , Saintive Simone , Goudouris Ekaterini Simoes , do Prado Evandro Alves , Fragoso dos Santos Henrique , da Silva Pereira Raphael , Cavalcante Fernanda Sampaio , de Carvalho Ferreira Dennis , dos Santos Kátia Regina Netto
Published: January 2024

Background

Atopic dermatitis (AD) patients have high rates of colonization by Staphylococcus aureus, which has been associated with worsening of the disease. This study characterized Staphylococcus spp isolates recovered from nares and feces of pediatric patients with AD in relation to antimicrobial susceptibility, staphylococcal cassette chromosome mec (SCCmec) type, presence of pvl genes and clonality. Besides, gut bacterial community profiles were compared with those of children without AD.

Results

All 55 AD patients evaluated had colonization by Staphylococcus spp. Fifty-three (96.4%) patients had colonization in both clinical sites, whereas one patient each was not colonize in the nares or gut. Staphylococcus aureus was identified in the nostrils and feces of 45 (81.8%) and 39 (70.9%) patients, respectively. Methicillin-resistant Staphylococcus spp. isolates were found in 70.9% of the patients, and 24 (43.6%) had methicillin-resistant S. aureus (MRSA). S. aureus (55.6%) and S. epidermidis (26.5%) were the major species found. The prevalent lineages of S. aureus were USA800/SCCmecIV (47.6%) and USA1100/SCCmecIV (21.4%), and 61.9% of the evaluated patients had the same genotype in both sites. Additionally, gut bacterial profile of AD patients exhibits greater dissimilarity from the control group than it does among varying severities of AD.

Conclusions

High rates of nasal and intestinal colonization by S. aureus and methicillin-resistant staphylococci isolates were found in AD patients. Besides, gut bacterial profiles of AD patients were distinctly different from those of the control group, emphasizing the importance of monitoring S. aureus colonization and gut microbiome composition in AD patients.