bluuebunny/crossref_metadata_2025 huggingface dataset:
Trend Analysis of Average Frequency Using Toothbrushing per Day in South Korea: An Observational Study of the 2010 to 2018 KNHANES Data
Authors: Kim Yu-Rin , Kang Hyun-Kyung
Published: March 2021

The aim of this study was to examine the trend of average frequency of toothbrushing per day according to the sociodemographic characteristics using the 5th, 6th, and 7th Korean National Health and Nutrition Examination Survey (KNHANES) data for 9 years; from 2010 to 2018. It intends to understand the state of toothbrushing practices in South Korea, and to provide basic data for promoting toothbrushing practices. Data from the 5th, 6th, and 7th KNHANESs conducted by the Korean Disease Control and Prevention Agency each year was analyzed using IBM SPSS version 26.0 (IBM Co., Armonk, NY, USA). Tableau version 2020.1 was used for the graphs and geographic information system (GIS). The significance level of α was set at 0.05 for testing. In all analyses, the complex sampling analysis method with stratification variables, cluster variables, and weights was applied, and the complex sample linear regression analysis. The average frequency of toothbrushing per day was higher in women for all the years; these women belonged to the age group under 65 and the employed group. The average frequency of toothbrushing per day was lower in the married, elementary school graduate, part-time job, and low-income groups for all years. The demographic and social factors affecting the number of toothbrushing practices per day were gender, education level, income level, and work type. After analyzing the average frequency of toothbrushing per day in each of the cities and provinces, Gangwon-do and Jeonbuk showed the highest increase in the frequency of toothbrushing in 2018 compared to 2010, whereas Incheon showed the lowest increase. From the above results, the average frequency of toothbrushing per day was lower in males, those with a low education level, the unemployed, and those in the rural area for a period of 9 years. Therefore, in-depth oral health promotion projects and national benefit policies should be considered for effective toothbrushing education by identifying individuals who do not brush their teeth.
Determinants of Disability in Minority Populations in Spain: A Nationwide Study
Authors: Casillas-Clot Javier , Pereyra-Zamora Pamela , Nolasco Andreu
Published: March 2021

Some population groups could be especially vulnerable to the effects of population ageing. The Global Activity Limitation Indicator (GALI) has been proposed as a measure of disability, but it has not been used in minority groups. The aim of this study is to estimate the prevalence of disability using the GALI and to analyse its determinants in immigrant and Roma populations. Data from the Spanish National Health Survey 2017 and the National Health Survey of the Roma Population 2014 were used, including adults aged 50 and above. Prevalence of disability was estimated, and odds ratios were calculated using logistic regression models to assess the association between disability and demographic, socioeconomic, and health variables. The prevalence of disability was estimated at 39.4%, 30.6%, and 58.7% in the native, immigrant, and Roma populations, respectively. Gender was a common determinant for the native and Roma populations. On the other hand, among immigrants, the risk of disability increased over the time residing in Spain. There were significant interactions with age and gender in the native population. Disability has different determinants in the three population groups. Public health measures to protect the Roma population and immigrants’ health should be considered.
Genotype to Phenotype: CRISPR Gene Editing Reveals Genetic Compensation as a Mechanism for Phenotypic Disjunction of Morphants and Mutants
Authors: Salanga Cristy M. , Salanga Matthew C.
Published: March 2021

Forward genetic screens have shown the consequences of deleterious mutations; however, they are best suited for model organisms with fast reproductive rates and large broods. Furthermore, investigators must faithfully identify changes in phenotype, even if subtle, to realize the full benefit of the screen. Reverse genetic approaches also probe genotype to phenotype relationships, except that the genetic targets are predefined. Until recently, reverse genetic approaches relied on non-genomic gene silencing or the relatively inefficient, homology-dependent gene targeting for loss-of-function generation. Fortunately, the flexibility and simplicity of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system has revolutionized reverse genetics, allowing for the precise mutagenesis of virtually any gene in any organism at will. The successful integration of insertions/deletions (INDELs) and nonsense mutations that would, at face value, produce the expected loss-of-function phenotype, have been shown to have little to no effect, even if other methods of gene silencing demonstrate robust loss-of-function consequences. The disjunction between outcomes has raised important questions about our understanding of genotype to phenotype and highlights the capacity for compensation in the central dogma. This review describes recent studies in which genomic compensation appears to be at play, discusses the possible compensation mechanisms, and considers elements important for robust gene loss-of-function studies.
Recent Advances in the Detection of Antibiotic and Multi-Drug Resistant Salmonella: An Update
Authors: Wu Siying , Hulme John P.
Published: March 2021

Antibiotic and multi-drug resistant (MDR) Salmonella poses a significant threat to public health due to its ability to colonize animals (cold and warm-blooded) and contaminate freshwater supplies. Monitoring antibiotic resistant Salmonella is traditionally costly, involving the application of phenotypic and genotypic tests over several days. However, with the introduction of cheaper semi-automated devices in the last decade, strain detection and identification times have significantly fallen. This, in turn, has led to efficiently regulated food production systems and further reductions in food safety hazards. This review highlights current and emerging technologies used in the detection of antibiotic resistant and MDR Salmonella.
Jasmonic Acid Pathway in Plants 2.0
Authors: Gomi Kenji
Published: March 2021

The plant hormone jasmonic acid (JA) and its derivative, an amino acid conjugate of JA (jasmonoyl isoleucine: JA-Ile), are signaling compounds involved in the regulation of cellular defense and development in plants [...]
Pathophysiology of Atherosclerotic Plaque Development-Contemporary Experience and New Directions in Research
Authors: Kowara Michal , Cudnoch-Jedrzejewska Agnieszka
Published: March 2021

Atherosclerotic plaque is the pathophysiological basis of important and life-threatening diseases such as myocardial infarction. Although key aspects of the process of atherosclerotic plaque development and progression such as local inflammation, LDL oxidation, macrophage activation, and necrotic core formation have already been discovered, many molecular mechanisms affecting this process are still to be revealed. This minireview aims to describe the current directions in research on atherogenesis and to summarize selected studies published in recent years—in particular, studies on novel cellular pathways, epigenetic regulations, the influence of hemodynamic parameters, as well as tissue and microorganism (microbiome) influence on atherosclerotic plaque development. Finally, some new and interesting ideas are proposed (immune cellular heterogeneity, non-coding RNAs, and immunometabolism) which will hopefully bring new discoveries in this area of investigation.
Percutaneous Transhepatic Electrohydraulic Lithotripsy for the Treatment of Difficult Bile Stones
Authors: Ierardi Anna Maria , Rodà Giovanni Maria , Di Meglio Letizia , Pellegrino Giuseppe , Cantù Paolo , Dondossola Daniele , Rossi Giorgio , Carrafiello Gianpaolo
Published: March 2021

Objectives: To evaluate the efficacy and safety of percutaneous transhepatic lithotripsy (PTL) using an electro-hydraulic (EH) system for difficult bile stones. Methods: We retrospectively evaluated two patients with recurrent cholangitis, jaundice and fever for the presence of difficult bile stones, inaccessible by an endoscopic approach, treated with PTL. Both procedures were conducted using the same protocol, with two different accesses. The treatments were performed using a 10 Fr flexible choledoscopy SpyGlass DSTM for visualization and an EH system for lithotripsy. Results: Technical success, clinical success and complications were evaluated. The two procedures were successfully concluded in both patients without any residual stones in the biliary tree. For both patients, a short follow-up period of six months was available, during which they remained asymptomatic. Neither major nor minor complications were registered. Conclusion: PTL was determined to be an effective and safe technique. This procedure allows a direct visualization of the stone, reducing fluoroscopy time and permitting a less invasive and less traumatic method for the percutaneous management of difficult bile stones. Advances in knowledge: The direct visualization, the high quality of the digital view, the adequate length of the device and the less traumatic approach of EH systems represent advantages compared with other available technologies.
Recent Progress in Oculopharyngeal Muscular Dystrophy
Authors: Yamashita Satoshi
Published: March 2021

Oculopharyngeal muscular dystrophy (OPMD) is a late-onset intractable myopathy, characterized by slowly progressive ptosis, dysphagia, and proximal limb weakness. It is caused by the abnormal expansion of the alanine-encoding (GCN)n trinucleotide repeat in the exon 1 of the polyadenosine (poly[A]) binding protein nuclear 1 gene (11–18 repeats in OPMD instead of the normal 10 repeats). As the disease progresses, the patients gradually develop a feeling of suffocation, regurgitation of food, and aspiration pneumonia, although the initial symptoms and the progression patterns vary among the patients. Autologous myoblast transplantation may provide therapeutic benefits by reducing swallowing problems in these patients. Therefore, it is important to assemble information on such patients for the introduction of effective treatments in nonendemic areas. Herein, we present a concise review of recent progress in clinical and pathological studies of OPMD and introduce an idea for setting up a nation-wide OPMD disease registry in Japan. Since it is important to understand patients’ unmet medical needs, realize therapeutically targetable symptoms, and identify indices of therapeutic efficacy, our attempt to establish a unique patient registry of OPMD will be a helpful tool to address these urgent issues.
Genetics of Acromegaly and Gigantism
Authors: Bogusławska Anna , Korbonits Márta
Published: March 2021

Growth hormone (GH)-secreting pituitary tumours represent the most genetically determined pituitary tumour type. This is true both for germline and somatic mutations. Germline mutations occur in several known genes (AIP, PRKAR1A, GPR101, GNAS, MEN1, CDKN1B, SDHx, MAX) as well as familial cases with currently unknown genes, while somatic mutations in GNAS are present in up to 40% of tumours. If the disease starts before the fusion of the epiphysis, then accelerated growth and increased final height, or gigantism, can develop, where a genetic background can be identified in half of the cases. Hereditary GH-secreting pituitary adenoma (PA) can manifest as isolated tumours, familial isolated pituitary adenoma (FIPA) including cases with AIP mutations or GPR101 duplications (X-linked acrogigantism, XLAG) or can be a part of systemic diseases like multiple endocrine neoplasia type 1 or type 4, McCune–Albright syndrome, Carney complex or phaeochromocytoma/paraganglioma-pituitary adenoma association. Family history and a search for associated syndromic manifestations can help to draw attention to genetic causes; many of these are now tested as part of gene panels. Identifying genetic mutations allows appropriate screening of associated comorbidities as well as finding affected family members before the clinical manifestation of the disease. This review focuses on germline and somatic mutations predisposing to acromegaly and gigantism.
Hypoparathyroidism in Pregnancy and Lactation: Current Approach to Diagnosis and Management
Authors: Ali Dalal S. , Dandurand Karel , Khan Aliya A.
Published: March 2021

Background: Hypoparathyroidism is an uncommon endocrine disorder. During pregnancy, multiple changes occur in the calcium-regulating hormones, which may affect the requirements of calcium and active vitamin D during pregnancy in patients with hypoparathyroidism. Close monitoring of serum calcium during pregnancy and lactation is ideal in order to optimize maternal and fetal outcomes. In this review, we describe calcium homeostasis during pregnancy in euparathyroid individuals and also review the diagnosis and management of hypoparathyroidism during pregnancy and lactation. Methods: We searched the MEDLINE, CINAHL, EMBASE, and Google scholar databases from 1 January 1990 to 31 December 2020. Case reports, case series, book chapters, and clinical guidelines were included in this review. Conclusions: During pregnancy, rises in 1,25-dihydroxyvitamin D (1,25-(OH)2-D3) and PTH-related peptide result in suppression of PTH and enhanced calcium absorption from the bowel. In individuals with hypoparathyroidism, the requirements for calcium and active vitamin D may decrease. Close monitoring of serum calcium is advised in women with hypoparathyroidism with adjustment of the doses of calcium and active vitamin D to ensure that serum calcium is maintained in the low-normal to mid-normal reference range. Hyper- and hypocalcemia should be avoided in order to reduce the maternal and fetal complications of hypoparathyroidism during pregnancy and lactation. Standard of care therapy consisting of elemental calcium, active vitamin D, and vitamin D is safe during pregnancy.
SOX11, SOX10 and MITF Gene Interaction: A Possible Diagnostic Tool in Malignant Melanoma
Authors: Beleaua Marius-Alexandru , Jung Ioan , Braicu Cornelia , Milutin Doina , Gurzu Simona
Published: March 2021

Malignant melanoma (MM) is a highly heterogenic tumor whose histological diagnosis might be difficult. This study aimed to investigate the diagnostic and prognostic utility of the conventional pan-melanoma cocktail members (HMB-45, melan-A and tyrosinase), in conjunction with SOX10 and SOX11 immunohistochemical (IHC) expression. In 105 consecutive cases of MMs and 44 of naevi, the IHC examination was performed using the five-abovementioned markers, along with microphthalmia transcription factor (MITF), S100, and Ki67. Correlation with the clinicopathological factors and a long-term follow-up was also done. Survival analysis was performed with Kaplan–Meier curves and compared with TCGA public datasets. None of the 44 naevi expressed SOX11, but its positivity was seen in 52 MMs (49.52%), being directly correlated with lymphovascular invasion, the Ki67 index, and SOX10 expression. HMB-45, SOX10, and tyrosinase, but not melan-A, proved to differentiate the naevi from MMs successfully, with high specificity. Triple MITF/SOX10/SOX11 co-expression was seen in 9 out of 15 negative conventional pan-melanoma-cocktail cases. The independent prognostic value was proved for the conventional pan-melanoma cocktail (triple positivity for HMB-45, melan-A, and tyrosinase) and, independently for HMB-45 and tyrosinase, but not for melan-A, SOX10, or SOX11. As consequence, to differentiate MMs from benign naevi, melan-A should be substituted by SOX10 in the conventional cocktail. Although the conventional pan-melanoma cocktail, along with S100 can be used for the identification of melanocytic origin of tumor cells and predicting prognosis of MMs, the conventional-adapted cocktail (triple positivity for HMB-45, SOX10, and tyrosinase) has a slightly higher diagnostic specificity. SOX11 can be added to identify the aggressive MMs with risk for lymphatic dissemination and the presence of circulating tumor cells.
On (k,p)-Fibonacci Numbers
Authors: Bednarz Natalia
Published: March 2021

In this paper, we introduce and study a new two-parameters generalization of the Fibonacci numbers, which generalizes Fibonacci numbers, Pell numbers, and Narayana numbers, simultaneously. We prove some identities which generalize well-known relations for Fibonacci numbers, Pell numbers and their generalizations. A matrix representation for generalized Fibonacci numbers is given, too.
A Generalized Weighted Monte Carlo Calibration Method for Derivative Pricing
Authors: Gudmundsson Hilmar , Vyncke David
Published: March 2021

The weighted Monte Carlo method is an elegant technique to calibrate asset pricing models to market prices. Unfortunately, the accuracy can drop quite quickly for out-of-sample options as one moves away from the strike range and maturity range of the benchmark options. To improve the accuracy, we propose a generalized version of the weighted Monte Carlo calibration method with two distinguishing features. First, we use a probability distortion scheme to produce a non-uniform prior distribution for the simulated paths. Second, we assign multiple weights per path to fit with the different maturities present in the set of benchmark options. Our tests on S&P500 options data show that the new calibration method proposed here produces a significantly better out-of-sample fit than the original method for two commonly used asset pricing models.
Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice
Authors: Guo Chih-Hung , Hsia Simon , Chung Chieh-Han , Lin Yi-Chun , Shih Min-Yi , Chen Pei-Chung , Hsu Guoo-Shyng W. , Fan Ciou-Ting , Peng Chia-Lin
Published: March 2021

Fish oil (FO) and selenium (Se) possess antiangiogenic potential in malignant tumors. This study aimed to determine whether combination of FO and Se enhanced treatment efficacy of low-dose antiangiogenic agent Avastin (bevacizumab) in a dose-dependent manner and targeted multiple signaling pathways in triple-negative breast cancer (TNBC)-bearing mice. Randomized into five groups, mice received treatment with either physiological saline (control), Avastin alone, or Avastin in combination with low, medium, and high doses of FO/Se. The target signaling molecules for anticancer were determined either by measuring protein or mRNA expression. Avastin-treated mice receiving FO/Se showed lower tumor growth and metastasis than did mice treated with Avastin alone. Combination-treated mice exhibited lower expressions in multiple proangiogenic (growth) factors and their membrane receptors, and altered cytoplasmic signaling molecules (PI3K-PTEN-AKT-TSC-mTOR-p70S6K-4EBP1, Ras-Raf-MEK-ERK, c-Src-JAK2-STAT3-TMEPAI-Smad, LKB1-AMPK, and GSK3β/β-catenin). Dose-dependent inhibition of down-stream targets including epithelial-to-mesenchymal transition transcription factors, nuclear cyclin and cyclin-dependent kinases, cancer stem cell markers, heat shock protein (HSP-90), hypoxia-inducible factors (HIF-1α/-2α), matrix metalloprotease (MMP-9), and increased apoptosis were observed. These results suggest that combination treatment with FO and Se increases the therapeutic efficacy of Avastin against TNBC in a dose-dependent manner through multiple signaling pathways in membrane, cytoplasmic, and nucleic targets.
Practical Considerations of Wastewater–Seawater Integrated Reverse Osmosis: Design Constraint by Boron Removal
Authors: Lee Chulmin , Kang Yesol , Kim Dong-Ho , Kim In S.
Published: March 2021

The wastewater–seawater (WW-SW) integrated reverse osmosis (RO) process has gained much attention in and out of academia due to its energy saving capability, economic benefits, and sustainability. The other advantage of this process is to reduce boron concentration in the RO permeate that can exclude the post-treatment process. However, there are multiple design constraints regarding boron removal that restrict process design in the WW-SW integrated system. In this study, uncertainties in design factors of the WW-SW integrated system in consideration of boron removal have been explored. In comprehensive consideration of the blending ratio of between WW and SW, regulatory water quality standard, specific energy consumption (SEC), specific water cost, and RO recovery rate, a range of 15,000~20,000 mg/L feed turned out to be the most appropriate. Furthermore, boron rejection tests with SWRO (seawater reverse osmosis) and BWRO (brackish water reverse osmosis) membranes under actual WW-SW integration found a critical reduction in boron rejection at less than 20 bar of operating pressure. These findings emphasize the importance of caution in the use of BWRO membranes in the WW-SW integrated RO system.
A First-Principles Study of Hydrogen Desorption from High Entropy Alloy TiZrVMoNb Hydride Surface
Authors: Zhang Jinjing , Hu Jutao , Xiao Haiyan , Shen Huahai , Xie Lei , Sun Guangai , Zu Xiaotao
Published: March 2021

The desorption behaviors of hydrogen from high entropy alloy TiZrVMoNb hydride surface have been investigated using the density functional theory. The (110) surface has been determined to be the most preferable surface for hydrogen desorption from TiZrVMoNb hydride. Due to the high lattice distortion and heterogeneous chemical environment in HEA hydride, hydrogen desorption from the HEA hydride surface is found to be complex. A comparison of molecular and atomic hydrogen desorption reveals that hydrogen prefers to desorb in atomic states from TiZrVMoNb hydride (110) surface rather than molecular states during the hydrogen desorption process. To combine as H2 molecules, the hydrogen atoms need to overcome attractive interaction from TiZrVMoNb hydride (110) surface. These results suggest that the hydrogen desorption on TiZrVMoNb hydride (110) surface is a chemical process. The presented results provide fundamental insights into the underlying mechanism for hydrogen desorption from HEA hydride surface and may open up more possibilities for designing HEAs with excellent hydrogen desorption ability.
Changes in Invasive Pneumococcal Disease Caused by Streptococcus pneumoniae Serotype 1 following Introduction of PCV10 and PCV13: Findings from the PSERENADE Project
Authors: Bennett Julia , Hetrich Marissa , Garcia Quesada Maria , Sinkevitch Jenna , Deloria Knoll Maria , Feikin Daniel , Zeger Scott , Kagucia Eunice , Cohen Adam , Ampofo Krow , Brandileone Maria-Cristina , Bruden Dana , Camilli Romina , Castilla Jesús , Chan Guanhao , Cook Heather , Cornick Jennifer , Dagan Ron , Dalby Tine , Danis Kostas , de Miguel Sara , De Wals Philippe , Desmet Stefanie , Georgakopoulou Theano , Gilkison Charlotte , Grgic-Vitek Marta , Hammitt Laura , Hilty Markus , Ho Pak-Leung , Jayasinghe Sanjay , Kellner James , Kleynhans Jackie , Knol Mirjam , Kozakova Jana , Kristinsson Karl , Ladhani Shamez , MacDonald Laura , Mackenzie Grant , Mad’arová Lucia , McGeer Allison , Mereckiene Jolita , Morfeldt Eva , Mungun Tuya , Muñoz-Almagro Carmen , Nuorti J. , Paragi Metka , Pilishvili Tamara , Puentes Rodrigo , Saha Samir , Sahu Khan Aalisha , Savrasova Larisa , Scott J. , Skoczyńska Anna , Suga Shigeru , van der Linden Mark , Verani Jennifer , von Gottberg Anne , Winje Brita , Yildirim Inci , Zerouali Khalid , Hayford Kyla ,   the PSERENADE Team
Published: March 2021

Streptococcus pneumoniae serotype 1 (ST1) was an important cause of invasive pneumococcal disease (IPD) globally before the introduction of pneumococcal conjugate vaccines (PCVs) containing ST1 antigen. The Pneumococcal Serotype Replacement and Distribution Estimation (PSERENADE) project gathered ST1 IPD surveillance data from sites globally and aimed to estimate PCV10/13 impact on ST1 IPD incidence. We estimated ST1 IPD incidence rate ratios (IRRs) comparing the pre-PCV10/13 period to each post-PCV10/13 year by site using a Bayesian multi-level, mixed-effects Poisson regression and all-site IRRs using a linear mixed-effects regression (N = 45 sites). Following PCV10/13 introduction, the incidence rate (IR) of ST1 IPD declined among all ages. After six years of PCV10/13 use, the all-site IRR was 0.05 (95% credibility interval 0.04–0.06) for all ages, 0.05 (0.04–0.05) for <5 years of age, 0.08 (0.06–0.09) for 5–17 years, 0.06 (0.05–0.08) for 18–49 years, 0.06 (0.05–0.07) for 50–64 years, and 0.05 (0.04–0.06) for ≥65 years. PCV10/13 use in infant immunization programs was followed by a 95% reduction in ST1 IPD in all ages after approximately 6 years. Limited data availability from the highest ST1 disease burden countries using a 3 + 0 schedule constrains generalizability and data from these settings are needed.
Configuration of Gut Microbiota Structure and Potential Functionality in Two Teleosts under the Influence of Dietary Insect Meals
Authors: Panteli Nikolas , Mastoraki Maria , Lazarina Maria , Chatzifotis Stavros , Mente Eleni , Kormas Konstantinos Ar. , Antonopoulou Efthimia
Published: March 2021

Insect meals are considered promising, eco-friendly, alternative ingredients for aquafeed. Considering the dietary influence on establishment of functioning gut microbiota, the effect of the insect meal diets on the microbial ecology should be addressed. The present study assessed diet- and species-specific shifts in gut resident bacterial communities of juvenile reared Dicentrarchus labrax and Sparus aurata in response to three experimental diets with insect meals from three insects (Hermetia illucens, Tenebrio molitor, Musca domestica), using high-throughput Illumina sequencing of the V3–V4 region of the 16S rRNA gene. The dominant phyla were Firmicutes, Proteobacteria and Actinobacteria in all dietary treatments. Anaerococcus sp., Cutibacterium sp. and Pseudomonas sp. in D. labrax, and Staphylococcus sp., Hafnia sp. and Aeromonas sp. in S. aurata were the most enriched shared species, following insect-meal inclusion. Network analysis of the dietary treatments highlighted diet-induced changes in the microbial community assemblies and revealed unique and shared microbe-to-microbe interactions. PICRUSt-predicted Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were significantly differentiated, including genes associated with metabolic pathways. The present findings strengthen the importance of diet in microbiota configuration and underline that different insects as fish feed ingredients elicit species-specific differential responses of structural and functional dynamics in gut microbial communities.
Phylogeny, Global Biogeography and Pleomorphism of Zanclospora
Authors: Réblová Martina , Kolařík Miroslav , Nekvindová Jana , Miller Andrew N. , Hernández-Restrepo Margarita
Published: March 2021

Zanclospora (Chaetosphaeriaceae) is a neglected, phialidic dematiaceous hyphomycete with striking phenotypic heterogeneity among its species. Little is known about its global biogeography due to its extreme scarcity and lack of records verified by molecular data. Phylogenetic analyses of six nuclear loci, supported by phenotypic data, revealed Zanclospora as highly polyphyletic, with species distributed among three distantly related lineages in Sordariomycetes. Zanclospora is a pleomorphic genus with multiple anamorphic stages, of which phaeostalagmus-like and stanjehughesia-like are newly discovered. The associated teleomorphs were previously classified in Chaetosphaeria. The generic concept is emended, and 17 species are accepted, 12 of which have been verified with DNA sequence data. Zanclospora thrives on decaying plant matter, but it also occurs in soil or as root endophytes. Its global diversity is inferred from metabarcoding data and published records based on field observations. Phylogenies of the environmental ITS1 and ITS2 sequences derived from soil, dead wood and root samples revealed seven and 15 phylotypes. The field records verified by DNA data indicate two main diversity centres in Australasia and Caribbean/Central America. In addition, environmental ITS data have shown that Southeast Asia represents a third hotspot of Zanclospora diversity. Our data confirm that Zanclospora is a rare genus.
Preparation and Characterization of Graphene from Refined Benzene Extracted from Low-Rank Coal: Based on the CVD Technology
Authors: Wu Dun , Wang Meichen , Zeng Jiawei , Yao Jinyuan , Jia Cheng , Zhang Hui , Li Jiangtao
Published: March 2021

Industrial preparation of graphene has been a research hotspot in recent years. Finding an economical and practical carbon source and reducing the cost of production and instrument is significant in industrial graphene production. Coal is a common carbon source. Efficient improvement and utilization in the cleaning of coal has recently been a popular research area. In this study, we developed a set of graphene preparation methods based on Anhui Huainan’s low-rank gas coal (HNGC). Using self-built experimental equipment, benzene precursor was prepared from HNGC and used as carbon source to realize graphene growth. The quality of the graphene was characterized by a high-resolution microscope and Raman spectrometer. This study provides a new idea and method for the preparation of low-rank coal-based graphene.
La Crosse Virus Shows Strain-Specific Differences in Pathogenesis
Authors: Wilson Sarah N. , López Krisangel , Coutermash-Ott Sheryl , Auguste Dawn I. , Porier Danielle L. , Armstrong Philip M. , Andreadis Theodore G. , Eastwood Gillian , Auguste Albert J.
Published: March 2021

La Crosse virus (LACV) is the leading cause of pediatric viral encephalitis in North America, and is an important public health pathogen. Historically, studies involving LACV pathogenesis have focused on lineage I strains, but no former work has explored the pathogenesis between or within lineages. Given the absence of LACV disease in endemic regions where a robust entomological risk exists, we hypothesize that some LACV strains are attenuated and demonstrate reduced neuroinvasiveness. Herein, we compared four viral strains representing all three lineages to determine differences in neurovirulence or neuroinvasiveness using three murine models. A representative strain from lineage I was shown to be the most lethal, causing >50% mortality in each of the three mouse studies. However, other strains only presented excessive mortality (>50%) within the suckling mouse neurovirulence model. Neurovirulence was comparable among strains, but viruses differed in their neuroinvasive capacities. Our studies also showed that viruses within lineage III vary in pathogenesis with contemporaneous strains, showing reduced neuroinvasiveness compared to an ancestral strain from the same U.S. state (i.e., Connecticut). These findings demonstrate that LACV strains differ markedly in pathogenesis, and that strain selection is important for assessing vaccine and therapeutic efficacies.
A Novel Infection Protocol in Zebrafish Embryo to Assess Pseudomonas aeruginosa Virulence and Validate Efficacy of a Quorum Sensing Inhibitor In Vivo
Authors: Nogaret Pauline , El Garah Fatima , Blanc-Potard Anne-Béatrice
Published: March 2021

The opportunistic human pathogen Pseudomonas aeruginosa is responsible for a variety of acute infections and is a major cause of mortality in chronically infected cystic fibrosis patients. Due to increased resistance to antibiotics, new therapeutic strategies against P. aeruginosa are urgently needed. In this context, we aimed to develop a simple vertebrate animal model to rapidly assess in vivo drug efficacy against P. aeruginosa. Zebrafish are increasingly considered for modeling human infections caused by bacterial pathogens, which are commonly microinjected in embryos. In the present study, we established a novel protocol for zebrafish infection by P. aeruginosa based on bath immersion in 96-well plates of tail-injured embryos. The immersion method, followed by a 48-hour survey of embryo viability, was first validated to assess the virulence of P. aeruginosa wild-type PAO1 and a known attenuated mutant. We then validated its relevance for antipseudomonal drug testing by first using a clinically used antibiotic, ciprofloxacin. Secondly, we used a novel quorum sensing (QS) inhibitory molecule, N-(2-pyrimidyl)butanamide (C11), the activity of which had been validated in vitro but not previously tested in any animal model. A significant protective effect of C11 was observed on infected embryos, supporting the ability of C11 to attenuate in vivo P. aeruginosa pathogenicity. In conclusion, we present here a new and reliable method to compare the virulence of P. aeruginosa strains in vivo and to rapidly assess the efficacy of clinically relevant drugs against P. aeruginosa, including new antivirulence compounds.
Bacteriophages of Shiga Toxin-Producing Escherichia coli and Their Contribution to Pathogenicity
Authors: Rodríguez-Rubio Lorena , Haarmann Nadja , Schwidder Maike , Muniesa Maite , Schmidt Herbert
Published: March 2021

Shiga toxins (Stx) of Shiga toxin-producing Escherichia coli (STEC) are generally encoded in the genome of lambdoid bacteriophages, which spend the most time of their life cycle integrated as prophages in specific sites of the bacterial chromosome. Upon spontaneous induction or induction by chemical or physical stimuli, the stx genes are co-transcribed together with the late phase genes of the prophages. After being assembled in the cytoplasm, and after host cell lysis, mature bacteriophage particles are released into the environment, together with Stx. As members of the group of lambdoid phages, Stx phages share many genetic features with the archetypical temperate phage Lambda, but are heterogeneous in their DNA sequences due to frequent recombination events. In addition to Stx phages, the genome of pathogenic STEC bacteria may contain numerous prophages, which are either cryptic or functional. These prophages may carry foreign genes, some of them related to virulence, besides those necessary for the phage life cycle. Since the production of one or more Stx is considered the major pathogenicity factor of STEC, we aim to highlight the new insights on the contribution of Stx phages and other STEC phages to pathogenicity.
Ethnobotanical Study on Plant Used by Semi-Nomad Descendants’ Community in Ouled Dabbeb—Southern Tunisia
Authors: Karous Olfa , Ben Haj Jilani Imtinen , Ghrabi-Gammar Zeineb
Published: March 2021

Thanks to its geographic location between two bioclimatic belts (arid and Saharan) and the ancestral nomadic roots of its inhabitants, the sector of Ouled Dabbeb (Southern Tunisia) represents a rich source of plant biodiversity and wide ranging of ethnobotanical knowledge. This work aims to (1) explore and compile the unique diversity of floristic and ethnobotanical information on different folk use of plants in this sector and (2) provide a novel insight into the degree of knowledge transmission between the current population and their semi-nomadic forefathers. Ethnobotanical interviews and vegetation inventories were undertaken during 2014–2019. Thirty informants aged from 27 to 84 were interviewed. The ethnobotanical study revealed that the local community of Ouled Dabbeb perceived the use of 70 plant species belonging to 59 genera from 31 families for therapeutic (83%), food (49%), domestic (15%), ethnoveterinary (12%), cosmetic (5%), and ritual purposes (3%). Moreover, they were knowledgeable about the toxicity of eight taxa. Nearly 73% of reported ethnospecies were freely gathered from the wild. The most commonly used plant parts were leaves (41%) followed by flowers and inflorescence (16%). We reported the use and collection of non-renewable parts (underground storage organs and roots) for 20 ethnospecies. Interestingly, a comparison with the available literature in Tunisia and neighboring countries reveals 13 new useful plants as well as 17 plants with new uses and demonstrates an important reservoir of traditional ethnobotanical heritage that is still sustained by respondents stemming from the semi-nomadic lifestyle of their ancestors (74% of cited taxa). These data could set a basis for further phytochemical and pharmacological research and conservative approach of the most relevant plant species including endemic overused and endangered taxa.
RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges
Authors: Gebremichael Daniel Endale , Haile Zeraye Mehari , Negrini Francesca , Sabbadini Silvia , Capriotti Luca , Mezzetti Bruno , Baraldi Elena
Published: March 2021

Plant pathogenic fungi are the largest group of disease-causing agents on crop plants and represent a persistent and significant threat to agriculture worldwide. Conventional approaches based on the use of pesticides raise social concern for the impact on the environment and human health and alternative control methods are urgently needed. The rapid improvement and extensive implementation of RNA interference (RNAi) technology for various model and non-model organisms has provided the initial framework to adapt this post-transcriptional gene silencing technology for the management of fungal pathogens. Recent studies showed that the exogenous application of double-stranded RNA (dsRNA) molecules on plants targeting fungal growth and virulence-related genes provided disease attenuation of pathogens like Botrytis cinerea, Sclerotinia sclerotiorum and Fusarium graminearum in different hosts. Such results highlight that the exogenous RNAi holds great potential for RNAi-mediated plant pathogenic fungal disease control. Production of dsRNA can be possible by using either in-vitro or in-vivo synthesis. In this review, we describe exogenous RNAi involved in plant pathogenic fungi and discuss dsRNA production, formulation, and RNAi delivery methods. Potential challenges that are faced while developing a RNAi strategy for fungal pathogens, such as off-target and epigenetic effects, with their possible solutions are also discussed.
Polymeric Materials Used for Immobilisation of Bacteria for the Bioremediation of Contaminants in Water
Authors: Berillo Dmitriy , Al-Jwaid Areej , Caplin Jonathan
Published: March 2021

Bioremediation is a key process for reclaiming polluted soil and water by the use of biological agents. A commonly used approach aims to neutralise or remove harmful pollutants from contaminated areas using live microorganisms. Generally, immobilised microorganisms rather than planktonic cells have been used in bioremediation methods. Activated carbon, inorganic minerals (clays, metal oxides, zeolites), and agricultural waste products are acceptable substrates for the immobilisation of bacteria, although there are limitations with biomass loading and the issue with leaching of bacteria during the process. Various synthetic and natural polymers with different functional groups have been used successfully for the efficient immobilisation of microorganisms and cells. Promise has been shown using macroporous materials including cryogels with entrapped bacteria or cells in applications for water treatment and biotechnology. A cryogel is a macroporous polymeric gel formed at sub-zero temperatures through a process known as cryogelation. Macroporous hydrogels have been used to make scaffolds or supports for immobilising bacterial, viral, and other cells. The production of composite materials with immobilised cells possessing suitable mechanical and chemical stability, porosity, elasticity, and biocompatibility suggests that these materials are potential candidates for a range of applications within applied microbiology, biotechnology, and research. This review evaluates applications of macroporous cryogels as tools for the bioremediation of contaminants in wastewater.
Resilience-Enhancing Solution to Mitigate Risk for Sustainable Supply Chain—An Empirical Study of Elevator Manufacturing
Authors: Hsu Chih-Hung , Yu Ru-Yue , Chang An-Yuan , Chung Wen-Hong , Liu Wan-Ling
Published: March 2021

As the complexity of supply chains increases, the enhancement of resilience for mitigating sustainable disruption risks in supply chains is an important issue. Quality function deployment (QFD) has been successfully applied in many domains to solve multicriteria decision-making (MCDM) problems. However, research on developing two houses of quality to connect sustainable supply chain disruption risks, resilience capacities, and resilience-enhancing features in elevator manufacturing supply chains by using the MCDM approach is lacking. This study aims to develop a framework for exploring useful decision-making by integrating the MCDM approach and QFD. By applying the framework, supply chain resilience can be improved by identifying the major sustainable risks and the key resilience to mitigate these risks. Important managerial insights and practical implications are obtained from the framework implementation in a case study of the elevator manufacturing industry. To strengthen resilience and thus mitigate key risks, the most urgent tasks are to connect the working site and the backstage to enhance product development and design and to share real-time job information. When these features are strengthened, agility, capacity, and visibility can be improved. Finally, unexpected events lead to changes in supplier delivery dates, and factors such as typhoon and lack of critical capacities/skilled employees with the greatest impact can be alleviated. This framework will provide an effective and pragmatic approach for constructing sustainable supply chain risk resilience in the elevator manufacturing industry.
Orders of Hunger and Heaven: Neoliberalism, Christian Charity and Homelessness in Taiwan
Authors: Beck Scott R.
Published: March 2021

Based on an ethnographic study of a Christian charity in Taipei, Taiwan, this paper examines how the mixing of “orders of worth” (Boltanski and Thevenot) is negotiated among charity workers and homeless people in the field setting. The organization, Grace Home Church, has two official goals: (1) to glorify God; (2) to assist homeless people. This mix of sacred and secular purposes often produces tensions, with the fundamental tension being between what the charity seeks to provide (salvation) versus what the homeless commonly want to be provided (food). As an analytic tool, I utilize Boltanski and Thevenot’s framework to link emergent tensions with broader social forces, such as neoliberalism, the welfare state, and religion. I will argue that charity workers as well as homeless individuals who have accepted Christianity attempt to separate the market and inspired orders through signifying practices that maintain a symbolic order, thereby justifying a sacred mission (for the charity organization) and self-worth (for the homeless).
High Spatial and Temporal Resolution Energy Flux Mapping of Different Land Covers Using an Off-the-Shelf Unmanned Aerial System
Authors: Simpson Jake E. , Holman Fenner , Nieto Hector , Voelksch Ingo , Mauder Matthias , Klatt Janina , Fiener Peter , Kaplan Jed O.
Published: March 2021

With the development of low-cost, lightweight, integrated thermal infrared-multispectral cameras, unmanned aerial systems (UAS) have recently become a flexible complement to eddy covariance (EC) station methods for mapping surface energy fluxes of vegetated areas. These sensors facilitate the measurement of several site characteristics in one flight (e.g., radiometric temperature, vegetation indices, vegetation structure), which can be used alongside in-situ meteorology data to provide spatially-distributed estimates of energy fluxes at very high resolution. Here we test one such system (MicaSense Altum) integrated into an off-the-shelf long-range vertical take-off and landing (VTOL) unmanned aerial vehicle, and apply and evaluate our method by comparing flux estimates with EC-derived data, with specific and novel focus on heterogeneous vegetation communities at three different sites in Germany. Firstly, we present an empirical method for calibrating airborne radiometric temperature in standard units (K) using the Altum multispectral and thermal infrared instrument. Then we provide detailed methods using the two-source energy balance model (TSEB) for mapping net radiation (Rn), sensible (H), latent (LE) and ground (G) heat fluxes at <0.82 m resolution, with root mean square errors (RMSE) less than 45, 37, 39, 52 W m−2 respectively. Converting to radiometric temperature using our empirical method resulted in a 19% reduction in RMSE across all fluxes compared to the standard conversion equation provided by the manufacturer. Our results show the potential of this UAS for mapping energy fluxes at high resolution over large areas in different conditions, but also highlight the need for further surveys of different vegetation types and land uses.
ME-Net: A Deep Convolutional Neural Network for Extracting Mangrove Using Sentinel-2A Data
Authors: Guo Mingqiang , Yu Zhongyang , Xu Yongyang , Huang Ying , Li Chunfeng
Published: March 2021

Mangroves play an important role in many aspects of ecosystem services. Mangroves should be accurately extracted from remote sensing imagery to dynamically map and monitor the mangrove distribution area. However, popular mangrove extraction methods, such as the object-oriented method, still have some defects for remote sensing imagery, such as being low-intelligence, time-consuming, and laborious. A pixel classification model inspired by deep learning technology was proposed to solve these problems. Three modules in the proposed model were designed to improve the model performance. A multiscale context embedding module was designed to extract multiscale context information. Location information was restored by the global attention module, and the boundary of the feature map was optimized by the boundary fitting unit. Remote sensing imagery and mangrove distribution ground truth labels obtained through visual interpretation were applied to build the dataset. Then, the dataset was used to train deep convolutional neural network (CNN) for extracting the mangrove. Finally, comparative experiments were conducted to prove the potential for mangrove extraction. We selected the Sentinel-2A remote sensing data acquired on 13 April 2018 in Hainan Dongzhaigang National Nature Reserve in China to conduct a group of experiments. After processing, the data exhibited 2093 × 2214 pixels, and a mangrove extraction dataset was generated. The dataset was made from Sentinel-2A satellite, which includes five original bands, namely R, G, B, NIR, and SWIR-1, and six multispectral indices, namely normalization difference vegetation index (NDVI), modified normalized difference water index (MNDWI), forest discrimination index (FDI), wetland forest index (WFI), mangrove discrimination index (MDI), and the first principal component (PCA1). The dataset has a total of 6400 images. Experimental results based on datasets show that the overall accuracy of the trained mangrove extraction network reaches 97.48%. Our method benefits from CNN and achieves a more accurate intersection and union ratio than other machine learning and pixel classification methods by analysis. The designed model global attention module, multiscale context embedding, and boundary fitting unit are helpful for mangrove extraction.