bluuebunny/crossref_metadata_2025 huggingface dataset:
MODIFIKASI ELEKTRODA PASTA KARBON (EPK) MENGGUNAKAN GRAFENA OKSIDA (GO) UNTUK PENENTUAN BUTIL HIDROKSI ANISOL SECARA VOLTAMMETRI
Authors: Fadillah Ganjar , Putri Elsa Ninda Karlinda , Febrianastutib Syahna
Published: October 2019

Butil hidroksi anisol (BHA) merupakan senyawa aditif yang banyak ditemukan dalam sediaan bahan pangan dan memberikan efek karsinogenik pada jumlah yang besar. Mengingat pentingnya analisis senyawa tersebut dan regulasi yang cukup ketat maka pada penelitian ini telah dikembangkan metode analisis berbasis elektrometri yaitu modifikasi elektroda pasta karbon (EPK) dengan grafena oksida (GO) untuk meningkatkan sensitivitas pengukuran. GO dipreparasi menggunakan metode Hammer yang dimodifikasi dan dikarakterisasi menggunakan x-ray diffraction (XRD) dan fourier transform infra red (FTIR). Hasil pengukuran analit menggunakan differential pulse voltammetry (DPV) menunjukan bahwa adanya modifikasi tersebut dapat meningkatkan respon arus pengukuran yang dihasilkan dari 11,70 μA menjadi 31,21 μA dalam larutan BHA 0,5 mM dengan elektrolit pendukung PBS 0,1 M pH 7. Hasil uji hipotesis  menunjukkan bahwa adanya nilai beda yang signifikan antara EPK/GO dengan EPK tanpa modifikasi terhadap respon arus pengukuran analit BHA.
Studi Teoritis Senyawa Turunan Kalkon Hidroksi Sebagai Sensor Kimia Berbagai Anion
Authors: Perdana Fitra , Wijaya Karna , Armunanto Ria
Published: October 2019

AbstrakStudi secara teoritis telah dilakukan untuk mempelajari interaksi senyawa turunan kalkon hidroksi (CH) sebagai sensor kimia dengan anion F-, Cl-, Br-, CN-, CH3COO-, dan NO3-. Penelitian ini bertujuan untuk memodelkan struktur molekul senyawa turunan kalkon hidroksi (CH) dan sifat sensornya serta model interaksinya dengan anion dengan metode DFT. Hasil optimasi geometri menunjukkan terjadinya pemanjangan ikatan O-H sebesar 0,63-0,85 Å dengan adanya anion F-, CN-, and CH3COO-. Hal ini mengindikasikan terjadinya deprotonasi sensor kalkon hidroksi (CH) oleh anion. Sedangkan interaksi sensor kalkon hidroksi (CH) dengan anion Cl-, Br-, dan NO3- hanya berupa ikatan hidrogen dengan pemanjangan ikatan O-H sebesar 0,08-0,46 Å. Sensor kalkon hidroksi (CH) yang mengalami deprotonasi memiliki energi interaksi yang lebih besar yaitu -209,37 – -424,06 kJ/mol dibandingkan sensor yang hanya membentuk ikatan hidrogen yaitu -98,60 – -125,59 kJ/mol. Sensor yang mengalami deprotonasi mengakibatkan turunnya selisih energi HOMO-LUMO dari -3,94 – -4,06 eV menjadi -2,62 – -2,75 eV.Kata kunci: sensor kimia, kalkon hidroksi, anion, DFT  AbstractTheoretical study had been carried out to investigate interaction between hydroxy chalcone derivatives (CH) as chemosensors with F-, Cl-, Br-, CN-, CH3COO-, and NO3- anions. This study was aimed to design molecular structures of chemosensor hydroxy chalcone (CH) properties for the anions and their interaction models with DFT method. The result of geometry optimization showed 0,63-0,85 Å O-H bond elongation of the hydroxy chalcone was occured by F-, CN-, and CH3COO-. It was indicated deprotonation of sensor hydroxy chalcone (CH) by anions. Where as the interaction of the hydroxy chalcone (CH) with Cl-, Br-, and NO3- just formed an hydrogen bond with 0,08-0,46 Å O-H bond elongation. Deprotonized sensor hydroxy chalcone (CH) had more energy change of interaction -209,37– -424,06 kJ/mol than sensor which just formed the hydrogen bond -98,60 – -125,59 kJ/mol. Deprotonized sensor caused reduction of the difference of HOMO-LUMO energy from - 3,94 – -4,06 eV to -2,62 – -2,75 eV.Keywords: chemosensor, hydroxy chalcone, anion, DFT
Initial Characterization of Alkaline Protease from Pseudomonas sp Isolated from Chicken Feces (POSTER)
Authors: Nuzulah Yuyun Firdausi , Suharti Suharti
Published: October 2019

Abstract Proteases are ubiquitous enzymes and it can be found in all living organisms. Extracellular proteases have high economic value, where the enzymes can be applied to various industrial sectors. In order to find an alternative source of protease, a search for microbe producing extracellular protease has been done using a selective medium. An extracellular producing bacterium that is identified as Pseudomonas sp has been isolated from chicken feces, that is Gallus gallus bankiva, collected from residential areas at Lowokwaru, Malang, Indonesia. The analysis shows that the bacterium produces extracellular protease with optimum activity after incubation of 66 hours at 35oC. Initial characterization of the extracellular protease shows that the extracellular protease produced by Pseudomonas sp has an optimum activity at pH 12 and an optimum temperature at 39oC. At these conditions, the crude extract enzyme has a specific activity of 0,522 U/mg. The high pH resistance allows its application to variety of industrial processes that require high pH operation. Therefore further studies need to be done to get better understanding of enzyme properties.  Keywords: Alkaline protease, Chicken feces, Gallus gallus bankiva, Pseudomonas sp
Precipitation Solvents for RNA Extraction of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, and Ultrapure H2O
Authors: Putri Rizqidhana Juliana , Sucipto Teguh Hari , Setyawati Harsasi , Churrotin Siti , Amarullah Ilham Harlan , Wardhani Puspa , Aryati Aryati , Soegijanto Soegeng
Published: October 2019

Dengue is a disease caused by a virus from the family Flaviviradae, carried by a female mosquito of Aedes aegypti species. Dengue fever is widespread in the tropic areas. It caused by rainfall, temperature and unplanned urbanization. According to the ministry of health , almost all provinces in Indonesia are endemic areas of dengue fever. In 2014, up to mid-December Dengue Hemorrhagic Fever (DHF) patients in 34 provinces in Indonesia are 71,668 people and 641. This figure is lower than the previous year, 2013 with 112,511 people and 871 deaths . This disease consists of four types of serotypes, namely DENV-1, DENV-2, DENV-3, and DENV-4. This disease can be identified using a variety of methods, one of the method is Reverse Transcription - Polymerase Chain Reaction (RT-PCR) method. This study aims to determine the ability of Dimethylformamide (DMF), Ethylenediamintetraacetic Acid (EDTA), and Ultrapure H2O as the substitute of  Ethanol for precipitation in RNA extraction process. The sample used in this research obtained from Surabaya. RNA extraction itself can be done by using a special kit for RNA extraction. In Reverse Transcription - Polymerase Chain Reaction method, first RNA is extracted and then transcribed back (Reverse Transcription) which then form cDNA that later will be amplified by using PCR method. In this study used specific primers for dengue virus type 3 (DENV-3). The results of this study show that DMF, EDTA, and Ultrapure H2O can be used as the substitute of Ethanol for precipitation on RNA extraction. The result is evidenced by the formation of viral DNA bands on gel electrophoresis results.
AKTIVITAS SITOTOKSIK EKSTRAK SELADA MERAH (Lactuca sativa var. Crispa) PADA BERBAGAI PELARUT EKSTRAKSI
Authors: Rohmah Jamilatur , Rini Chylen Setiyo , Wulandari Fitria Eka
Published: October 2019

Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder dan aktivitas sitotoksik dengan nilai LC50dari ekstrak selada merah (Lactuca sativa var.Crispa) pada berbagai pelarut. Sampel selada merah yang digunakan didapatkan dari desa Turirejo kecamatan Lawang-Malang. Simplisia selada merah di ekstraksi dengan metode maserasi menggunakan variasi pelarut yaitu pelarut etanol, metanol, etil asetat, dan n-heksana selama 24 jam. Hasil maserasi dipekatkan dengan alat rotary vacuum evaporator. Kemudian dilakukan uji skrining fitokimia serta KLT untuk mengetahui kandungan metabolit sekunder dalam sampel. Pengujian aktivitas sitotoksik dilakukan dengan metode BSLT (Brine Shrimp Lethality Test). Hasil ekstraksi didapatkan ekstrak kental etanol, metanol, etil asetat, dan n-heksana secara berturut-turut sebesar 17,30 g; 12,32 g; 25,61 g; dan 3,76 g. Dan hasil dari uji fitokimia menunjukkan ekstrak etanol selada merah mengandung flavonoid, tanin, saponin, triterpenoid, steroid dan alkaloid. Sedangkan ekstrak metanol selada merah mengandung alkaloid, saponin, dan tanin. Pada ekstrak etil asetat selada merah mengandung alkalid, saponin, steroid, dan tanin. Dan pada ekstrak n-heksana selada merah mengandung alkaloid, steroid, triterpenoid, dan tanin. Hasil aktivitas sitotoksik ekstrak metanol memiliki bioaktivitas tertinggi terhadap Artemia salina Leach. dibandingkan dengan ekstrak etanol, etil asetat, dan n-heksana. Nilai LC50ekstrak etanol, metanol, etil asetat, dan n-heksana secara berturut-turut adalah 322,288 ppm; 68,632 ppm; 1934,287 ppm; dan 170,115 ppm.Kata kunci: Selada merah (Lactuca sativa var. Crispa), sitotoksik, Artemia salina Leach, BSLT.
SINTESIS, KARAKTERISASI DAN UJI AKTIVITAS SENYAWA KOMPLEKS Zn(II)-KATEKIN SEBAGAI INHIBITOR ENZIM LIPASE
Authors: Putri Antyka Lutfiana , Sumarsih Sri , Setyawati Harsasi
Published: October 2019

ABSTRACT The study aims to synthesis, characterization and activity testing of Zn(II)-catechin as lipase inhibitor activity. The complex compound from synthesis result is characterized by its maximum wavelength, functional groups, metals and ligands bonds, and it’s melting point. This complex compound tested of Zn(II)-catechin as lipase inhibitor activity to p-NPP (para-nitrofenilpalmitat) substrate. The result shows that Zn(II)-catechin could be synthesized from Zn(II) ligans and metals with ratio ligands and metals mol is 1:1, that has maximum wavelength 454 nm, which contain Zn-O bond at 354,90 and 478,35 cm-1, and melting point of Zn(II)-catechin >250 oC. Zn(II)-catechin can inhibition 60,63% from concentration 50 µg/mL with mixed inhibition Keyword : Zn(II)-catechin, lipase, mixed inhibition ABSTRAK Penelitian ini bertujuan untuk melakukan sintesis, karakterisasi dan uji aktivitas senyawa kompleks Zn(II)-katekin terhadap aktivitas inhibitor enzim lipase. Senyawa kompleks hasil sintesis dikarakterisasi panjang gelombang maksimumnya, gugus fungsi serta ikatan logam dan ligannya, serta titik lelehnya. Senyawa kompleks diuji aktivitas inhibitor enzim lipase terhadap substrat p-NPP (paranitrofenilpalmitat). Hasil penelitian menunjukkan bahwa senyawa kompleks Zn(II)-katekin dapat disintesis dari ligan katekin dan logam Zn(II) dengan perbandingan mol ligan logam 1:1, mempunyai panjang gelombang maksimum 454 nm, mengandung ikatan Zn-O pada 354,90 dan 478,35 cm-1 dan titik leleh senyawa kompleks >250 oC. Senyawa kompleks Zn(II)-katekin pada konsentrasi 50µg/mL mempunyai daya inhinbisi sebesar 60,63% terhadap enzim lipase dengan jenis inhibisi campuran. Kata kunci : Zn(II)-katekin, lipase, inhibitor campuran
UJI SITOTOKSIK TERHADAP SINTESIS DAN KARAKTERISASI MAGNETIK NANOPARTIKEL CuFe2O4 YANG DILINGKUPI BOVINE SERUM ALBUMIN (BSA)
Authors: Usreg Handjani Sri Usreg , Husein Albari , Fahmi, Ph.D Mochamad Zakki
Published: October 2019

AbstrakNanopartikel, dalam beberapa tahun ini mendapatkan perhatian lebih dari peneliti dan ilmuan dunia, hal ini terlihat banyaknya jurnal maupun penelitian terutama dalam bidang kesehatan. Salah satu contoh aplikasi dalam bidang biomedis sebagai agen pengontras yaitu Magnetic Resonance Imaging (MRI). Nanopartikel magnetik CuFe2O4 telah berhasil disintesis yang berasal dari bahan Fe(acac)3 dan Cu(acac)2 dengan penambahan Bovine Serum Albumin sebagai nanocarrier. Sintesis dilakukan dengan mencampurkan bahan Fe(acac)3 dan Cu(acac)2, kemudian mereaksikannya secara solvothermal sehingga membentuk CuFe2O4. Proses selanjutnya mencampurkan larutan CuFe2O4 dan larutan BSA dalam air kemudian diultrasonikasi. Produk CuFe2O4-BSA selanjutnya dikarakterisasi dan diuji ketahanan terhadap perubahan pH dan penambahan garam. Hasil uji menunjukkan bahwa CuFe2O4-BSA dapat mempertahankan kestabilan, terbukti pada pH 3-12 selama 24 jam dan pada konsentrasi garam 0,5 M masih tetap stabil. Proses sintesis dapat dikatakan berhasil dilakukan karena hasil XRD menunjukkan terdapatnya senyawa CuFe2O4 sesuai database JCPDF 34-0425, dimana menunjukkan peak pada posisi pada 34,718º, 35,861º, dan 62,156º. Hasil karakterisasi menggunakan XRD menunjukkan bahwa peak produk muncul pada posisi 35.3896º dan 62.6159º. Hasil karakterisasi menggunakan DLS menunjukkan bahwa produk CuFe2O4-BSA memiliki rata-rata diameter partikel sebesar 153,2 nm. Hasil karakterisasi MSB menunjukkan bahwa CuFe2O4 memiliki momen magnet yang tinggi yaitu sebesar 1,57BM. Uji Sitotoksik CCK-8 menunjukkan nanopartikel magnetik CuFe2O4-BSA, secara keseluruhan memiliki sifat yang tidak toksik terhadap sel HeLa dibuktikan dengan nilai derajat viabilitas terendah memiliki nilai 82%. Katakunci: Nanopartikel Magnetik, CuFe2O4, Bovine Serum Albumin,Sitotoksik
ANALISIS CEMARAN LOGAM BERAT TEMBAGA (Cu) PADA AMDK DAN AMIU DI DAERAH PANYILEUKAN DENGAN MENGGUNAKAN SSA
Authors: Andriansyah Ivan , Yuliantini Anne , Yunita Avita Rischa
Published: October 2019

Air mineral merupakan salah satu kebutuhan manusia yang paling penting. Kebutuhan air minum dapat dipenuhi dari AMDK. Ada syarat yang harus dipenuhi oleh AMDK untuk menjamin kualitas air minum yang layak dikonsumsi. Salah satu syarat AMDK adalah tidak mengandung logam berat tembaga (Cu) yang lebih dari 0,5 bpj. Tembaga termasuk logam yang dibutuhkan oleh tubuh dalam jumlah sedikit tetapi dalam jumlah yang berlebihan akan bersifat toksik. Oleh karena itu, diperlukan analisis kadar Cu pada AMDK khususnya di daerah Panyileukan guna menjamin kualitas AMDK. Penelitian ini terdiri dari 2 tahapan, yaitu validasi, penetapan kadar sampel menggunakan SSA pada panjang gelombang 324,75 nm dan statistika. Dari hasil validasi, didapatkan persamaan kurva kalibrasi y = 0,1537x + 0,0134 dengan nilai koefisien korelasi (r) sebesar 0,998, batas deteksi dan kuantisasi berturut-turut sebesar  0,03 dan 0,12 bpj, persen perolehan kembali 0,5 – 1,7% dengan nilai SBR kurang dari 2 %. Hasil pengukuran kadar Cu pada AMDK berkisar antara 0,00100 – 0,00192 bpj. Dari data AMDK dapat dikatakan bahwa kadar Cu tidak melebihi ambang batas yang ditetapkan oleh SNI 01-3553-2006 yaitu 0,5bpj.
UJI PENGIKATAN KOLESTEROL OLEH EKSTRAK METANOL BEKATUL BERAS KETAN HITAM SECARA IN VITRO
Authors: Kurnia Dewi , Lisniawati Nenden Ayu , Dinata Deden Indra
Published: October 2019

Beras ketan  hitam merupakan varietas lokal yang mengandung pigmen paling baik, dibandingkan dengan beras putih atau beras warna lain. Warna hitam pada beras ketan hitam berasal dari lapisan bekatul dan bagian bulir berasnya yang mengandung pigmen antosianin berwarna ungu. Bekatul beras ketan hitam mengandung serat serealia yang baik bagi organ pencernaan. Pada penelitian ini dilakukan pengujian pengikatan kolesterol oleh ekstrak metanol bekatul beras ketan hitam. Ekstraksi dilakukan dengan cara maserasi menggunakan metanol 70% dalam kondisi asam (penambahan HCl 1%). Uji pengikatan kolesterol oleh ekstrak metanol bekatul beras ketan hitam dilakukan secara in vitro dengan metode pembentukan kompleks kolesterol dengan FeCl3. Serapan kolesterol bebas diukur menggunakan spektrofotometer UV-Vis pada serapan maksimum panjang gelombang 526 nm. Randemen ekstrak yang diperoleh adalah sebesar 17,53%. Identifikasi ekstrak menggunakan KLT dengan eluen BAA (4,5:1:4,5) menunjukan adanya senyawa golongan antosianin.  Hasil uji pengikatan kolesterol  menunjukkan bahwa ekstrak metanol bekatul beras ketan hitam dengan konsentrasi 20 ppm dapat menurunkan kolesterol sebesar 47,46%. Peningkatan konsentrasi ekstrak menunjukan semakin besar pula penurunan kadar kolesterol bebas.Kata kunci: bekatul beras ketan hitam, pengikatan kolesterol, in vitro, spetkrofotometer UV-Vis
How popular is Islamic finance in the USA? Findings from Google Trends
Authors: Aldarabseh Wesal M.
Published: October 2019

Islamic finance is a growing industry with global distribution in all continents including Europe and America. The aim of the current study was to examine how popular is Islamic finance in the USA during the period 2014-2019 using Google Trends. In addition, the interest in Islamic finance across different US states was also investigated. Using “Islamic finance” and “Islamic bank” as search terms in Goggle Trends, the trend curve showed decreases in search volumes, suggesting a decline in the popularity of Islamic finance in the USA with years. Search volumes were detected in seven out of 50 states, suggesting low interest in Islamic finance in the majority of US states. The order of the popularity in the seven states was: Virginia > New York > New Jersey > Illinois > Texas > California > Pennsylvania > Georgia > Florida > Massachusetts. Longitudinal survey studies are needed to confirm the present findings.
The components of intangible capital: An exploration of Lebanese companies
Authors: Bakhit Wael , Ziadeh Marwa , El Abiad Zouhour
Published: October 2019

The contemporary economy has changed dramatically and moved from the industrial age to the information age, pushing businesses to embark into a predominantly intangible economy. Thus, and in addition to solid and liquid value, there is a gaseous value which is the intangible capital. This exploratory study of 14 Lebanese companies analyzed with the “Tropes” software focuses on the constituent elements of intangible capital, its place within these companies and its contribution to the creation of value. This work concludes a first proposal for the classification of the components of intangible capital.
Pricing Policy and Its Impact on Company’s Profitability, Case of Kurdistan Region of Iraq\ Sulaimani
Authors: Rashid Chnar abdullah
Published: October 2019

This research project is mainly focused on analyzing pricing policy and its impact on company’s profitability through collecting receipts from 3 different places in Kurdistan Region of Iraq/ Sulaimani to evaluate their pricing policy and its impact on generating profits and increasing sales on the one hand, and interviewing some accounting, and economy’s experts on the other. A hypothesis has been developed in which pricing policy affects profits and sales. The results indicated that pricing policy has an impact on profits and sales. And further information has been provided in the research.
EXCHANGE RATE AND PERFORMANCE OF THE RESIDENTIAL PROPERTY MARKET IN KENYA
Authors: Njoroge Charles Gitiya , Muturi Willy , Oluoch Oluoch
Published: October 2019

The purpose of the study was to establish the effect of exchange rate on the performance of the residential property market in Kenya. The study used secondary data that was accumulated using secondary data collection sheet from first quarter of 2005 to fourth quarter of 2018. The study conducted several test statistics and diagnostic tests in order to achieve the most optimal solution. Vector error correction model and auto-regressive distributed lag model were employed to test the hypothesis in the short run and long run respectively. The results found out that exchange rate had a negative effect on performance of residential properties in Kenya in both the short run and positive effect in long run. The study has narrowed down the research gap brought about by the conflicting emprical, theoretical and conceptual literature with regard to the effect of exchange rate on performance of residential property market in Kenya.

Key Words: Exchange Rate, Performance, Residential Property
RELATED FACTORS OF RESPONSE TIME IN HANDLING HEAD INJURY IN EMERGENCY UNIT OF PROF.DR.H.M.ANWAR MAKKATUTU BANTAENG GENERAL HOSPITAL
Authors: Mudatsir Satrial , Sangkala Moh. Syafar , Setyawati Andina
Published: October 2019

Introduction: The mechanisms of nurse response time in handling head injury patients has an important role on the safety and viability of patients in reference to the rules of “safety and time saving is life saving”. The purpose of the study: This study aims to identify factors that related to the response time in handling head injury patient in emergency department (ED) and to determine the most related factor  to the response time. Method: This study was an observation design and using cross sectional approach. A total sample of 32 emergency department nurses participated for this study, in references to the inclision and exclusion criteria. The data were analyzed by using chi-square, Fisher's extract test and logistic regression. Result: This study found that there were statistical significance between respon time and  level of education (p = 0.006), duration of working (p = 0.005), medical emergency training (p = 0.001), the emergency department facilities (p = 0.008) and the level of the patient acute condition (p = 0.006). Among the factors, it was found that facilities most related factor to the respon time (OR = 6.945). Conclusion and suggestion: It is concluded that there is a relationship between respon time and education level, work duration of nurses, medical emergency training, Emergency facilities and the patient acute condition  in handling head injury patients and facilities are the factors that most related to the response time handling head injury. It is suggested to the hospital to complete the emergency room facilities because the facilities affects the response time handling head injury patients.
EFFECT OF INGESTING TRAINING TOWARDS DYSPHAGIA IN STROKE’ PATIENTS IN HAJI HOSPITAL AND MAKASSAR CITY HOSPITAL
Authors: Afrida Afrida
Published: October 2019

Introduction: Stroke is a loss of brain function caused by the cessation of blood supply to the brain. One of the most common clinical disorders of stroke is ingesting or dysphagia. This study aims to identify the effect of ingesting exercise to stroke patients with dysphagia in the General Hospital of Haji General Hospital of Makassar City. Method: The design of this study was pre experimental with One Group Pre-Post Test Design. Samples in this study were all stroke patients with dysphagia with Convenience Sampling technique, obtained 20 respondents. Rehabilitation of ingesting exercise is done three times a day at breakfast, lunch, and dinner for seven days in a row. Evaluation of respondents' ingesting status was done on the first day before exercise and last day after practicing exercise. Result: Bivariate results showed that there was an influence between stroke patients with dysphagia and ingesting exercises (p <0.001). Conclusion: It is further recommended that nurses perform structured ingesting exercises as self-care nursing intervention in dealing with stroke patients who have dysphagia.
EMOTIONAL QUESTION OF STUDENTS IN UNDERGRADUATE COURSE OF SCHOOL OF NURSING - HASANUDDIN UNIVERSITY
Authors: Hendra Desy Ana , Hapsah Hapsah , Malasari Silvia , Syam Yuliana , Andriani Andriani
Published: October 2019

Introduction: Lower level of caring in Indonesian nurses related to emotional intelligence level of nursing, and  assertion to nurses for always being professional in any circumstances and this condition relatated to emotional intelligence which is formed when undergoing nursing education along emotional intelligence phenomena  in nurses period of education and period of profession education  between regular students and non-regular students. Method: the design of this study is descriptive research with crossectional approach. Sample selection technique using disproportionate stratified random sampling, with the number of respondent about 128 respondents. Primary data was obtained through a TEIQUE questionnaire in the form of question about Emotional Itelligence and the ability of recognizing emotion, managing emotion, self motivation, empathy, social skill Result: This study shows that, 55 % respondents have a high emotional intelligence and higer in term of recognizing emotion ability (80.5 %), managing emotion ability (56.3 %), self motivation ability (92.2 %), empathy ability (59.4 %), and social skill ability (63.3 %). Respondent characteristics who have higer in emotional intelligence were from adults (56.4 %), woman (56.4 %), unreguler students (96.4 %), widow/widowed marriage status (69.2 %), student who do not entered any organization (76.1 %), and students in seventh grade (69.4 %).Conclusion: : the result showed that more than half respondents have higer emotional intelligence (managing emotion ability, self motivation ability, empathy ability, social skill ability ) so that give us information about description of Emotional Intelligence in Undergraduate Hasanuddin University Nursing Students.
NURSES’ PERCEPTIONS AND EXPECTATIONS ON THE IMPLEMENTATION OF CAREER LADDER IN PUBLIC HOSPITALS IN MAKASSAR
Authors: Pasang Meyke Tiku , Kadar Kusrini , Natzir Rosdiana
Published: October 2019

Introduction: Nurses as professionals must provide services in accordance with the competence and authority possessed either individually or as part of a hospital team. Thus, there is a need to manage the resources of nursing  such as the professional career development system for nurses. The study aimed to identify the perception and expetation of the nurses on the implementation of career ladder in two hospitals in Makassar city. Method: This research used quantitative method with the cross sectional design. There were 105 nurses from Hasanuddin University Hospital and 212 nurses from Dr. Wahidin Sudirohusodo Hospital involved as samples in this study. The samples were chosen using the disproportionate stratified random sampling technique. The data were collected through questionnaires to find out the nurse perception including some open ended  question to explore the nurses expectation from this career ladder program.  The data were  analyzed using descriptive statistic and mann-whitney to seee the differences perception between the groups. Result: The result revealed although that mean of perception about the implementation of career ladder in two hospitals are considered good (m=3.70 dan m=3.94), there was differences of nurses perception in both hospitals (p=0.00). Conclusions: Meanwhile, the result of open-ended questions about nurses' expectation about the implementation of the career ladder emphasized that there should  be a schedule for training for their professional improvement. Evaluation for further assessment for higher levels of career ladder also considered important for the nurses in both hospitals. Furthermore, the rewards or incentives for the nurses received shold be appropriate according to the career levels. In conclusion, the implementation of career ladder program in both hospitals have been considered good, however, the hospitals need to support the nurses and pay attention to factors that could influence the nurses satisfaction of the program.
DESCRIPTION OF ELDERLY MENTAL STATUS IN WORK AREA PUSKESMAS PAMPANG MAKASSAR
Authors: Wulandari Ayu , Syam Yuliana , Puspitha Arnis
Published: October 2019

Introduction:mental status of elderly is an emotional or cognitive state in the elderly is shown by the behavior of the stand. Mental health problems are very important for the elderly and often occurs in the elderly. The incidence of mental disorders in the elderly status is very high. paired mental status in elderly pose a variety of problems that can affect physical health, psychological, social and quality of life of the elderly so that the necessary nursing services for the early detection of mental status disorders that occur among the elderly. Method: The study design quantitative descriptive survey method. Samples numbered 97 people were selected using cluster random sampling. Primary data was collected by questionnaire the Mini Mental Status Examination (MMSE). Result:T he study of 97 respondents showed that 55.7% had normal mental status, impaired mental status light there are 29.9% and 14.4% of elderly with impaired mental status severe. Based propporsi elderly who suffer from mental status of the most widely experienced by women (52.4%), greater in very old age (100%), greater in elderly unmarried (100%), greater in elderly are not in school ( 70.6%), greater in elderly with a history of self-employed work (44.7%), and greater in the elderly with a history of rheumatic disease (61.1%). Conclusions: Mental status of elderly in Puskesmas Kota Makassar Pampang mostly normal. Therefore, elderly mental health need to be detected early to avoid the impact of mental disorders in the elderly.
THE CORRELATION OF TELEVISION SHOW AND BULLYING BEHAVIOR IN HIGH SCHOOL OF MAHAPUTRA IN MAKASSAR
Authors: Suryani Suryani , Andriani Andriani , Arafat Rosyidah
Published: October 2019

Introduction: Bullying is an experience  that occurs when the helplessness  persecuted  by the others behaviour and afraid of the repected bad behaviour and ill do it is repeated. One of causes bullying behavior is a television show, this can occur because television show that is presented is very diverse, so that school children is free to watch their favorite television show. This study aimed to determine the relationship between television show with bullying behavior in student of class XI and XII in SMA Maha Putra Makassar. Method: This study using  cross  sectional  method.  The  total  study  populations   130  and  samples  are  98 respondents with purposive sampling technique. Data analyzed were using the Chi-Square.  Result: In this study showed that there is no relationship  between television  show with bullying  behavior  in  student  of  class  XI  and  XII  in  Maha  Putra  Senior  High  School Makassar. Conclusions: Level bullying dominant is low level with the  bullying  often  is  verbal  bullying,  and  program  often  that  watched  is  soap  opera. Therefore, they are expected to do more introduce of bullying to students.
REPLACEMENT PERIPHERAL INTRAVENOUS CATHETERS WITH CLINICAL INDICATION FOR PATIENT CONVENIENCE, COST EFFICIENCY AND REDUCING NURSES WORKLOAD
Authors: Nurmawan Narmawan , Djafar Ida , Hakim Hasnani , Syafril Harmiati , Syahrul Syahrul , Ningrat Syahrul
Published: October 2019

Introduction: PIVCs (Peripheral Intravenous Catheters) have been widely used for clinical medical care which is having risk for phlebitis occurence. IV catheter replacement is performed when there is clinical indication such us phlebitis. The consequence of this action is patient convinence, nurse workload and patient care cost efficiency. Methode: A longitudinal study with experimental results of evidence based practice on replacement of PIVSc with clinical indication  as an effort to improve patient convinience, cost efficiency and reduce nurse workload in 41 patients. Result: 41 patients attached PIVCs, 100% wanted IV catheter replacement if there was any clinical indication. 68% of patients said very uncomfortable if IV catheter routinely replaced. 16 nurse (56.25%) said there is no need to replace IV catheter regularly. 43.75% of nurses felt IV catheters rountine replacement increased the working time and 50% of the nurses felt that nurses workload was increased. Conclusions: IV catheter replacement can be performed if there is cinical indication because it can improve patient convenience, increase cost efficiency and reduce nurse workload.
NURSES’ EXPERIENCE OF DEALING WITH PATIENTS’ AGGRESSIVE BEHAVIOUR IN PSYCHIATRIC EMERGENCY WARD OF HOSPITAL IN SOUTH SULAWESI
Authors: Hasnah Hasnah , Hapsah Hapsah , Malasari Silvia , Saleh Ariyanti , Harisa Akbar
Published: October 2019

Introduction: The high level of risk emergency psychiatry nurse becomes the target of aggressive behavior in patients both physically and psychologically, providing a special experience for nurses. Positive and negative impacts felt more likely a psychological effect on the nurse. Objective: Identifying the experiences of nurses dealing with patient of aggressive behavior in Emergency Psychiatric of Rumah Sakit Khusus Daerah Provinsi Sulawesi Selatan. Methods: The phenomenological approach to decision informants using purposive sampling with the informant as much as 9 informants who have experienced aggressive behavior from patients. Results: There were 7 themes obtained by the feelings of nurses with aggressive behavior patients, nurses experienced different kinds of aggressive behavior,  nurses identified about the causes of the patient's aggressive behavior, nurse found some signs of the patient while being aggressive, nurses perceived impact of agresive behavior after getting the incident of it, the way nurses treated patients aggressive behavior, and nurses found obstacle to overcome aggressive behavior's patient. The positive experience of nurses in dealing with patient of aggressive behavior was nurse know the proper ways, causes and signs of the patient while being aggressive so the nurse was able to anticipate the action will be carried out. On the other hand the negative experience felt by the nurse due to the impacts of the aggressive behavior patients. Conclusions and:  The experience felt by nurses in dealing with patients in Psychiatric Emergency Unit of Rumah Sakit Khusus Daerah Provinsi Sulawesi Selatan there were positive and negative. Therefore, we need a way to reduce aggressive behavior and provide management training for nurses in dealing with aggressive behavior in order to keep patients safely.
EFFECT OF 30 MINUTES WALKING ON BLOOD PRESSURE OF ELDERLY IN PACCERAKKANG DISTRICT OF MAKASSAR
Authors: Rezky Andi Nurfadilah , Nur Nurmiyanti , Samad Rismawati , Puspitha Arnis
Published: October 2019

Background: Sports activity affects a person's fitness level. Sports activities in the form of aerobic exercise, resistance or resistance training and flexibility exercises can improve your fitness. The exercise was done with medium intensity 3 times per week and 30 minutes duration. The importance of one's body fitness should get more attention. Especially in improving the quality of physical condition capabilities such as cardiovascular endurance, muscle strength and endurance, body shape. In this case a person can perform maximum exercise on the physical condition. This study aims to find out how the effect of walking 30 minutes to decrease blood pressure in elderly in working area of Health Center of Pacer Balik Kota Makassar. Method: This research is Quasy Experiment with Pre and Post Test Without Control design. The sample of research is 30 respondents. The research instrument is a tool to check uric acid levels and blood pressure measuring devices. Result: Blood pressure of respondent before walking done have mean value 3,40 mmHg and walking done have mean value 2,47 mmHg, Based on hypothesis test which have been done, hence obtained conclusion that walking 30 min influence significantly to pressure drop blood. Based on the calculation that has been done, obtained p-value for the pair of pre-posttest variable for blood pressure is 0.000 while the significance level α = 0.05. Conclusion and Suggestion: Based on hypothesis testing that has been done, hence obtained conclusion that walk 30 minutes influence significantly to decrease blood pressure. By knowing the influence of elderly gymnastics on the decrease of blood pressure in elderly with hypertension, nurses are expected to give and suggest non-pharmacological intervention and walk 3 times a week with 30 minutes duration in minimizing risk of complication of hypertension.
The Relations between Birth Weight and The Growth of Children under Five in Puskesmas Goarie Subdistrict Marioriwawo Regency Soppeng
Authors: Erika Kadek Ayu , Mulhaeriah Mulhaeriah , Amir Nurul Arifah
Published: October 2019

Introduction:The birth weight is one of health assessment and prediction of children under five in the future. Growth assessment can uses Denver II screening test to detect earlier the growth whether normal or it risks to delay in growing to know the relations between birth growth and children under five growth in Puskesmas Goarie Subdistrict Marioriwawo Regency Soppeng. Method:This research is quantitative research that uses predictive correlation design. The sample in this research is taken by using purposive sampling technique. The sample estimation is 90 children under five. Result:Most of toddlers in this research have the normal weight (74.4%). The majority of growth aspects of toddler are in the normal category, including hard motor (84,5%), soft motor (87,8%), language and speech (73,3%), social personal (73,3 %) also interpretation at the end of toddler growth are in the normal category (66,7%). There is a significant relation between the weight of birth and the growth of toddler p=0,0001 smaller than α (0,05). Conclusion:The result of this research in Community Health Centre of Goarie Subdistrict Marioriwawo Regency Soppeng, showed that the majority of toddler born with normal birth weight and in the normal category, and there is a relation between the weight of birth with the growth of toddler. Health workers are expected to be screened to assess the growth of toddler and also parents are expected to care for childs’ growth.
A MEASURING INSTRUMENT TO MEASURE NURSES’ KNOWLEDGE AND SKILLS IN IMPLEMENTING OF OF EVIDENCE-BASED PRACTICE: A LITERATURE REVIEW
Authors: HIDAYAT WAHYU , Syahrul Syahrul
Published: October 2019

Introduction: There are many types of instruments that are used as tools in measuring nurses' knowledge and skills related to EBP implementation. The purpose the study: This study aims to investigate the appropriate instruments used in measuring the knowledge and skills of nurses in the application of evidence-based practice. Method: Searching for resources using Pubmed and Willey databases then using Cross Sectional Critical Appraisal Skills Program (CASP) as a tool to extract the publications obtained. Result: 11 publications were analyzed, there were only 2 publications that used the same instrument. But that does not mean that the instrument used by other researchers is invalid. Conclusion and suggestion: This literature review cannot explain which instrument is better to use in measuring nurse knowledge and skills. The selection of the instruments to be used needs to pay attention to the suitability of the contents of the instrument with the organization's organizational culture so that the measurement results made can provide valid data.
Alteração do uso e ocupação do solo em Governador Celso Ramos/SC entre 1986 e 2019
Authors: Tramonte Flávia , Farina Karina
Published: October 2019

A colonização exerce uma tendência ao tipo de uso e ocupação do solo nas regiões costeiras. A falha na gestão destes locais influencia na degradação ambiental e diminuição na capacidade de suporte e regeneração natural dos ecossistemas. O objetivo geral da presente pesquisa é identificar as alterações do uso e ocupação do solo do município de Governador Celso Ramos/SC entre os anos de 1986 e 2019. Para analisar o uso e ocupação, utilizou-se imagens do satélite Landsat 5 e 8.. As imagens foram processadas utilizando o software Qgis 3.8 e complemento SCP. Identificou-se um aumento nas áreas urbanizadas e úmidas, enquanto houve diminuição das áreas com vegetação rasteira. Áreas de floresta aumentaram ao longo de três décadas. A inclusão da classe “Samambaiaarame” e o fato de esta espécie de samambaia estar presente em 2,2% do território em 2019 indica relação com a diminuição de áreas de vegetação rasteira, que incluem agricultura e pastos, por razão de a espécie ser bioindicadora de regeneração ambiental.
Análise da relação entre temperatura da superfície e NDVI nos anos 2015 e 2019 do município de Balneário Piçarras/SC
Authors: Correa da Silva Dayana Karina
Published: October 2019

O planejamento urbano é um instrumento contemplado no Estatuto das Cidades. Os municípios da zona costeira, com o aumento da população sofre com os impactos do uso do solo de forma desordenada. O presente artigo tem o objetivo de avaliar a cobertura vegetal do município de Balneário Piçarras/SC comparando com os dados de temperatura de superfície terrestre, verificando algumas hipóteses apontadas pelo senso comum. Para este artigo foram utilizadas imagens doLANDSAT 8, nas bandas 4 e 5 para cálculo do NVDI e banda 10 para mensurar a temperatura da superfície terrestre. Como resultados, foram confeccionados 4 mapas a partir das imagens de duas datas distintas (01.01.2015 e 28.01.2019) a fim de demonstrar a evolução do município em relação a estes parâmetros.
Avaliação Qualitativa da Expansão Urbana na Ilha de Santa Catarina
Authors: Cavalcanti da Rocha Eunesio , De Oliveira Roseli
Published: October 2019

O crescimento urbano, sempre foi um dos grandes desafios para gestão das cidades; a falta de um planejamento adequado e de políticas públicas é fator determinante para o aumento de conflitos, com os diversos atores sociais. A ocupação do solo urbano de forma inadequada traz problemas como: precária mobilidade urbana, grandes impactos ambientais, aglomerações inadequadas como moradias, entre outros. O objetivo deste trabalho é analisar a expansão urbana na RegiãoInsular de Florianópolis, sua configuração atual e os problemas advindos desta expansão, utilizando imagens de satélites, LANDSAT-5 e 8, de 1986 e 2018 adquiridas através do site Earth Explorer, e utilizando o programa QGIS, como ferramenta de geoprocessamento.
Avaliação da relação entre focos de Aedes aegypti com a distribuição espacial da temperatura no municipio de Itajaí/SC, por geoprocessamento
Authors: Matiola Cleusa
Published: October 2019

A dengue é uma doença viral que vem colocando em risco parte da população do mundo. O vírus é transmitido pelo Aedes aegypti, também transmissor do zika vírus e da febre chikungunya. O número de A. aegypti é significativo ao longo dos anos. As variações climáticas contribuem para o aumento do vetor. O objetivo deste estudo é avaliar a relação dos focos de A. aegypti com a temperatura, no município de Itajaí/SC, utilizando geoprocessamento, para um diagnóstico queleve em consideração as caraterísticas locais e sua influência no desenvolvimento do vetor. Para isto, foi realizado modelagem estatística dos focos de A. aegypti, georreferenciamento dos endereços dos focos e tratamento de imagens termais, banda 10 Landsat 8. Na análise espacial das imagens termais, verificou-se que a áreas que apresentaram temperaturas mais elevadas, também foram as áreas aonde se localizaram os focos do mosquito.
Apresentação, nasce a revista Metodologias e Aprendizado
Authors: Werneck Ribeiro Eduardo Augusto
Published: October 2019

Apresentação e proposta da revista
Comprehensive transcriptome analysis of grafting onto Artemisia scoparia W. to affect the aphid resistance of chrysanthemum (Chrysanthemum morifolium T. )
Authors: Zhang Xue-ying , Sun Xian-zhi , Zhang Sheng , Yang Jing-hui , Liu Fang-fang , Fan Jie
Published: October 2019

Abstract
Background: Aphid ( Macrosiphoniella sanbourni ) stress drastically influences the yield and quality of chrysanthemum, and grafting has been widely used to improve tolerance to biotic and abiotic stresses. However, the effect of grafting on the resistance of chrysanthemum to aphids remains unclear. Therefore, we used the RNA-Seq platform to perform a de novo transcriptome assembly to analyze the self-rooted grafted chrysanthemum ( Chrysanthemum morifolium T. 'Hangbaiju') and the grafted Artermisia-chrysanthemum (grafted onto Artemisia scoparia W.) transcription response to aphid stress.
Results : The results showed that there were 1337 differentially expressed genes (DEGs), among which 680 were upregulated and 667 were downregulated, in the grafted Artemisia-chrysanthemum compared to the self-rooted grafted chrysanthemum. These genes were mainly involved in sucrose metabolism, the biosynthesis of secondary metabolites, the plant hormone signaling pathway and the plant-to-pathogen pathway. KEGG and GO enrichment analyses revealed the coordinated upregulation of these genes from numerous functional categories related to aphid stress responses. In addition, we determined the physiological indicators of chrysanthemum under aphid stress, and the results were consistent with the molecular sequencing results. All evidence indicated that grafting chrysanthemum onto A. scoparia W. upregulated aphid stress responses in chrysanthemum.
Conclusion: In summary, our study presents a genome-wide transcript profile of the self-rooted grafted chrysanthemum and the grafted Artemisia-chrysanthemum and provides insights into the molecular mechanisms of C. morifolium T. in response to aphid infestation. These data will contribute to further studies of aphid tolerance and the exploration of new candidate genes for chrysanthemum molecular breeding.
Key words : Chrysanthemum, Grafting, Aphid stress, Gene expression, RNA-Seq
Time to viral load suppression and its associated factors in cohort of patients taking antiretroviral treatment in East Shewa Zone, Oromiya, Ethiopia, 2018
Authors: Ali Jemal H
Published: October 2019

Background

A key goal of ART is to achieve and maintain durable viral suppression. Thus, the most important use of the viral load measurement is to monitor the effectiveness of therapy after initiation of ART. The main objective of the study was to determine the time for virological suppression and its associated factors among people living with HIV taking antiretroviral treatments in East Shewa Zone, Oromiya, Ethiopia.Methods: Patients diagnosed with Human Immunodeficiency Virus presenting to the study health centers between October 3, 2011 and March 1, 2013 were included in the study given the following criteria: age 18 years or greater, eligible to start ART. All patients with baseline viral load measurement were included in the study. Interaction between explanatory variables with the response variable was analyzed by using cross tab features of SPSS, IBM Inc. Significance group comparison was done by Kaplan Meier log-rank test. Cox proportional hazard model was used to select significant factors to the variability between groups.Result: Plasma viral load was suppressed below the detection level in 72% of individuals taking different regimen of ART. The median HIV-1 plasma viral load in the cohort was estimated to be log 5.3111 copies/ml. Survival curve difference was observed in the category of marital status (p- value 0.023) and baseline CD4 value (p- value 0.023). The estimated median time to PVL suppression was 181days (CI: 140.5-221.4) with the age group of 30-39years having minimum time to achieve suppression with 92 days (CI: 60.1-123.8) and the maximum time required to reach the level was found among the age group between 50-59 years.Conclusion: The estimated time to achieve PVL after taking ART was found to be 181 days. Factors affecting time to suppression level was marital status and baseline CD4.
Keywords: Viral load, ART, Ethiopia, Suppression, HIV
GUCY2D Gene Loss-of-Function Mutations Responsible for Leber Congenital Amaurosis 1
Authors: Xue Feng , Wei Tianying , Sun Junhui , Luo Yuqin , Huo Yanan , Yu Ping , Chen Jiao , Wei Xiaoming , Qi Ming , Ye Yinghui
Published: October 2019

Background: Leber congenital amaurosis (LCA) is a group of severe congenital neurodegenerative diseases. Variants in the guanylate cyclase 2D gene ( GUCY2D ), which encodes guanylate cyclase 1 (ROS-GC1), are associated with LCA1 and account for 6%–21% of all LCA cases.
Methods: In this study, one family with LCA1 was recruited from China. A combination of next generation sequencing and Sanger sequencing was used to screen for disease-causing mutations. Additionally, immunohistochemistry and HPLC-coupled tandem mass spectrometry (HPLC-MS/MS) were used to confirm the cellular location and catalytic activity of ROS-GC1 mutants, respectively.
Results: We found three novel mutations (c.139_139delC, c.835G>A and c.2783G>A) in the GUCY2D gene. Mutation c.139_139delC results in a truncated protein. Mutations c.835G>A and c.2783G>A exert no effects on cellular location but reduce significantly the catalytic activity of ROS-GC1.
Conclusions: Our findings highlight the clinical range of LCA. Moreover, HPLC-MS/MS was used to analyze the concentration of 3',5'-cyclic guanosine monophosphate (cGMP), suggesting that HPLC-MS/MS is an effective alternative method to evaluate the catalytic activity of wild-type and mutant ROS-GC1

Keywords: Leber congenital amaurosis 1, GUCY2D , catalytic activity, 3',5'-cyclic guanosine monophosphate, guanylate cyclase 1