Electron microscopical studies of the common bile duct in reindeer
Authors: Rahko Timo , Nikander Sven 
Published: February 2013

<p>In a previous publication the authors have described some ultrastructural characteristics of granulated cells in the common bile duct of the reindeer. On the basis of the same material, electron microscopic observations on other tissue elements of bile duct wall are now reported. The surface and glandular epithelium were composed of tall columnar epithelial cells with villous structures on the luminal surfaces. The parietal cytoplasmic membranes of epithelial cells were equipped with intercellular desmosomes while intraepithelial globule leucocytes did not form any junctional complex with other cells. Apical cytoplasmic areas of superficial epithelial cells showed electron-dense small bodies possibly consisting of mucinous substances. The goblet and deep glandular cells, on the other hand, contained numerous large mucin granules with less electron-dense matrices. It appears that their secretions are more abundant than those in superficial epithelial cells which obviously are absorptive as their main function. The nuclei and other cytoplasmic organelles showed profiles similar to those in epithelial cells generally. The lumen of the bile ducts was usually empty or contained fine-granular or amorphous material. An unusual feature was the presence of parts of globule leucocytes or even almost whole cells occurring freely in ductal secretions.</p><p>Elektronimikroskooppinen tutkimus yhteisen sappik&auml;yt&auml;v&auml;n rakenteesta porolla.</p><p>Abstract in Finnish / Yhteenveto: Aikaisemmassa julkaisussa tekij&auml;t kuvasivat poron yhteisen sappik&auml;yt&auml;v&auml;n (<em>ductus hepaticus communis</em>) sein&auml;m&auml;n jyv&auml;sellisten solujen hienorakennetta. T&auml;ss&auml; artikkelissa selostetaan saman aineiston perusteella (6 tervett&auml; teurasporoa) elektronimikroskooppisia havaintoja sappik&auml;yt&auml;v&auml;sein&auml;m&auml;n muista kudosrakenteista. Sappik&auml;yt&auml;v&auml;sein&auml;m&auml;n pinta- ja rauhasepiteeli koostuu korkeista epiteelisoluista. Pinnallisia epiteelisoluja kattavat s&auml;&auml;nn&ouml;lliset mikrovillukset, ja niill&auml; on vain v&auml;h&auml;n ilmeisesti limaa sis&auml;lt&auml;vi&auml; jyv&auml;si&auml; solulimassaan. Rau-has- ja pikarisoluissa s&auml;&auml;nn&ouml;lliset mikrovillukset sen sijaan puuttuvat. Niiden sytoplasman t&auml;ytt&auml;v&auml;t runsaat li-mapalloset, joita solut muodostavat hyvin kehittyneess&auml; Golgin laitteessaan eritt&auml;en limaa sappik&auml;yt&auml;v&auml;n onte-loon. Erite n&auml;kyy hienojyv&auml;isen&auml; tai tasa-aineisena sein&auml;mi&auml; reunustavana aineena. Poikkeuksellisena havaintona voidaan pit&auml;&auml; ker&auml;ssolujen ker&auml;sten tai l&auml;hes kokonaisten ker&auml;ssolujen esiintymist&auml; sappik&auml;yt&auml;v&auml;on-teloissa mahdollisesti osoituksena solujen vaelluskyvyst&auml;. Vaikka epiteelisolut muodostavat lujia solukalvosi-doksia toisiinsa, ker&auml;ssolut eiv&auml;t kiinnittyneet muihin soluihin. Ilmeist&auml; on, ett&auml; pinnallisten epiteelisolujen toiminta on p&auml;&auml;asiassa absorptiivista, mutta rauhasepiteelisolut ovat erikoistuneet eritystoimintaan.</p><p>En elektronmikroskopisk studie av gallg&aring;ngen hos ren.</p><p>Abstract in Swedish / Sammandrag: Gallg&aring;ngarnas yt- och k&ouml;rtelepitel bestod av h&ouml;ga epitelceller bekl&auml;dda med ett regelbundet villusskikt. Intercellulara desmosomer s&aring;gs i epitelcellernas parietala cytoplasmamembraner. De intraepiteliala globulara leukocyterna saknade desmosomer eller andra bindningar med n&aring;rliggande celler. I de superficiala epitelcellernas apikala cytoplasma fanns elektrontata sm&aring; kroppar antagligen best&aring;ende av mucin. B&auml;gar- och de djupare belagna k&ouml;rtelcellerna inneholl rikligt med stora mucin granulor med ett mindre elektrontatt matrix. Det f&ouml;ref&ouml;ll som om dessa celler skulle vara sekrerande och de superficiala epitelcellerna absorberande. K&auml;rnen och andra cytoplasmatiska organeller hade egenskaper jamf&ouml;rbara med epitelceller i allm&auml;nhet. Gallg&aring;ngen var oftast tom men ibland s&aring;gs ett finkornigt amorft material i den. Som en anmarkningsvard observation ansees forekomsten av delar och t.o.m. hela globulara leukocyter i gallg&aring;ngen.</p>