bluuebunny/crossref_metadata_2025 huggingface dataset:
Mesothelial Cells Exhibit Characteristics of Perivascular Cells in an In Vitro Angiogenesis Assay
Authors: Koukorava Chrysa , Ward Kelly , Ahmed Katie , Almaghrabi Shrouq , Dauleh Sumaya , Pereira Sofia M. , Taylor Arthur , Haddrick Malcolm , Cross Michael J. , Wilm Bettina
Published: October 2023

Mesothelial cells have been shown to have remarkable plasticity towards mesenchymal cell types during development and in disease situations. Here, we have characterized the potential of mesothelial cells to undergo changes toward perivascular cells using an in vitro angiogenesis assay. We demonstrate that GFP-labeled mesothelial cells (GFP-MCs) aligned closely and specifically with endothelial networks formed when human dermal microvascular endothelial cells (HDMECs) were cultured in the presence of VEGF-A165 on normal human dermal fibroblasts (NHDFs) for a 7-day period. The co-culture with GFP-MCs had a positive effect on branch point formation indicating that the cells supported endothelial tube formation. We interrogated the molecular response of the GFP-MCs to the angiogenic co-culture by qRT-PCR and found that the pericyte marker Ng2 was upregulated when the cells were co-cultured with HDMECs on NHDFs, indicating a change towards a perivascular phenotype. When GFP-MCs were cultured on the NHDF feeder layer, they upregulated the epithelial–mesenchymal transition marker Zeb1 and lost their circularity while increasing their size, indicating a change to a more migratory cell type. We analyzed the pericyte-like behavior of the GFP-MCs in a 3D cardiac microtissue (spheroid) with cardiomyocytes, cardiac fibroblasts and cardiac endothelial cells where the mesothelial cells showed alignment with the endothelial cells. These results indicate that mesothelial cells have the potential to adopt a perivascular phenotype and associate with endothelial cells to potentially support angiogenesis.
Sleep Disordered Breathing in Children with Neuromuscular Disease
Authors: Chidambaram Ambika G. , Jhawar Sanjay , McDonald Craig M. , Nandalike Kiran
Published: October 2023

Sleep disordered breathing (SDB) in children with neuromuscular disease (NMD) is more prevalent compared to the general population, and often manifests as sleep-related hypoventilation, sleep-related hypoxemia, obstructive sleep apnea, central sleep apnea, and/or disordered control of breathing. Other sleep problems include, sleep fragmentation, abnormal sleep architecture, and nocturnal seizures in certain neuromuscular diseases. The manifestation of sleep disordered breathing in children depends on the extent, type, and progression of neuromuscular weakness, and in some instances, may be the first sign of a neuromuscular weakness leading to diagnosis of an NMD. In-lab diagnostic polysomnography (PSG) remains the gold standard for the diagnosis of sleep disordered breathing in children, but poses several challenges, including access to many children with neuromuscular disease who are non-ambulatory. If SDB is untreated, it can result in significant morbidity and mortality. Hence, we aimed to perform a comprehensive review of the literature of SDB in children with NMD. This review includes pathophysiological changes during sleep, clinical evaluation, diagnosis, challenges in interpreting PSG data using American Academy of Sleep (AASM) diagnostic criteria, management of SDB, and suggests areas for future research.
Effects of Probiotics in Adults with Gastroenteritis: A Systematic Review and Meta-Analysis of Clinical Trials
Authors: Mitra Amal K. , Asala Adetoun F. , Malone Shelia , Mridha Malay Kanti
Published: October 2023

Probiotics have been widely used in gastroenteritis due to acute and chronic illnesses. However, evidence supporting the effectiveness of probiotics in different health conditions is inconclusive and conflicting. The aim of this study was to review the existing literature on the effects of probiotics on gastroenteritis among adults. Only original articles on clinical trials that demonstrated the effects of probiotics in adults with gastroenteritis were used for this analysis. Multiple databases, such as PubMed, Google Scholar, MEDLINE and Scopus databases, were searched for the data. The study followed standard procedures for data extraction using a PRISMA flow chart. A quality appraisal of the selected studies was conducted using CADIMA. Finally, a meta-analysis was performed. Thirty-five articles met the selection criteria; of them, probiotics were found effective in the treatment and/or prevention of chronic inflammatory bowel disease (IBD), including ulcerative colitis and Crohn’s disease in 17 (49%), and the treatment of pouchitis in 4 (11.4%), antibiotic-induced diarrhea in 3 (8.6%), Helicobacter pylori infection in 2 (5.7%) and diverticulitis in 1 (2.9%), while the remaining 7 (20%) were ineffective, and 1 study’s results were inconclusive. The meta-analysis did not demonstrate any significant protective effects of probiotics. Having a τ2 value of zero and I2 of 6%, the studies were homogeneous and had minimum variances. Further studies are suggested to evaluate the beneficial effects of probiotics in IBDs and other chronic bowel diseases.
THANet: Transferring Human Pose Estimation to Animal Pose Estimation
Authors: Liao Jincheng , Xu Jianzhong , Shen Yunhang , Lin Shaohui
Published: October 2023

Animal pose estimation (APE) boosts the understanding of animal behaviors. Recent vision-based APE has attracted extensive attention due to the advantages of contactless and sensorless applications. One of the main challenges in APE is the lack of high-quality keypoint annotations for different animal species since manually annotating the animal keypoints is very expensive and time-consuming. Existing works alleviate this problem by synthesizing APE data and generating pseudo-labels for unlabeled animal images. However, feature representations learned from synthetic images could not be directly transferred to real-world scenarios, and the generated pseudo-labels are usually noisy, which limits the model’s performance. To address the above challenge, we propose a novel cross-domain vision transformer for APE to Transfer Human pose estimation to Animal pose estimation, termed THANet, as humans share skeleton similarities with some animals. Inspired by the success of ViTPose in HPE, we design a unified vision transformer encoder to extract universal features for both animals and humans followed by two task-specific decoders. We further introduce a simple but effective cross-domain discriminator to bridge the domain gaps between the human pose and the animal pose. We evaluated the proposed THANet on the AP-10K and Animal-Pose benchmarks, and the extensive experiments show that our method achieves a promising performance. Specifically, the proposed vision transformer and cross-domain method significantly improve the model’s accuracy and generalization ability for APE.
Characteristics of Biogas Production Activity and Microbial Community during Sub-Moderate Temperature Anaerobic Digestion of Wastewater
Authors: Wu Jingwei , Zhang Huan , Zhao Ye , Yuan Xufeng , Cui Zongjun
Published: October 2023

Sub-moderate temperature (ranging from low to moderate temperature) anaerobic digestion (AD) could balance fermentation efficiency and energy input. We investigated biogas production and the microbial community in wastewater AD at sub-moderate (15 °C, 20 °C, and 25 °C) and moderate (35 °C; control group) temperatures with the organic loading rate (OLR) incrementally increased over 200 days. The impact of temperature on biogas production was found to be minimal at a low OLR but became more significant at a high OLR. Notably, a temperature threshold ranging from 15 °C to 20 °C exerted a strong inhibitory effect on biogas production and disrupted the microbial community. And, SMT-AD is deemed by this study to be the optimal application strategy of wastewater with low temperature and low OLR. Bacterial richness was positively and linearly related to temperature. There is a relevance between methane production and archaeal diversity under the influence of temperature and OLR. Temperature and OLR shaped the ecological function of predominant bacteria. Anaerolineales, Thermotogales, and Lactobacillales were strongly influenced by temperature. Synergistales had a synergistic relationship with Desulfovibrionales. Clostridiales was responsible for acetate and butyrate production and closely related to Lactobacillales. Acetoclastic Methanosaetaceae was the predominant methanogen. Methanogens could survive and maintain their population even though methanogenesis was limited under high OLRs and low temperatures.
Effect of Spirulina in Bread Formulated with Wheat Flours of Different Alveograph Strength
Authors: Hernández-López Israel , Alamprese Cristina , Cappa Carola , Prieto-Santiago Virginia , Abadias Maribel , Aguiló-Aguayo Ingrid
Published: October 2023

Consumers within the EU are increasingly asking for natural and healthier food products, which are additive-free and environmentally friendly. The aim of this study was to assess the effects of Spirulina (Arthrospira sp.) in bread formulated with four wheat flours with different alveograph strengths. The flours used were Manitoba Flour (00/251), Ground-force wholemeal (Whole/126), Standard Bakery Flour (0/W105), and Organic Bakery Flour (2/W66). Powdered Spirulina biomass was used as a new ingredient with a high nutritional profile and bioactive compounds; incorporation was made at two levels: 1.5% and 2.5% of the flour amount. The same bread recipe was used for all formulations, but for the 1.5 and 2.5% variations, 6 g and 10 g of Spirulina were added, respectively. Antioxidant capacity increased with increasing microalgal biomass. The visual and taste attributes of the breads with microalgae underwent noticeable changes compared to their counterparts without microalgae. Biomass addition significantly (p < 0.05) affected bread weight and volume, and different trends were found based on the type of wheat flour. Spirulina-containing breads showed a greener coloration while the microalgae concentration was augmented. The moisture and texture were slightly affected by the addition of the biomass at both levels. The 2.5% concentration samples were well accepted in most cases by consumers, emphasizing the salty flavor as a pleasant feature. No significant sensory differences were observed between samples, and the acceptability index was always higher than 72%. The results show that Spirulina could be an environmentally friendly ingredient for the reformulation of nutritionally enhanced bread with a good texture that is well-accepted by consumers.
Three Dimensional Natures of Massive Star Envelopes
Authors: Jiang Yan-Fei
Published: October 2023

In this paper, we review our current understanding of the outer envelope structures of massive stars based on three-dimensional (3D) radiation hydrodynamic simulations. We briefly summarize the fundamental issues in constructing hydrostatic one-dimensional (1D) stellar evolution models when stellar luminosity approaches the Eddington value. Radiation hydrodynamic simulations in 3D covering the mass range from 13M⊙ to 80M⊙ always find a dynamic envelope structure with the time-averaged radial profiles matching 1D models with an adjusted mixing-length parameter when convection is subsonic. Supersonic turbulence and episodic mass loss are generally found in 3D models when stellar luminosity is super-Eddington locally due to the opacity peaks and convection being inefficient. Turbulent pressure plays an important role in supporting the outer envelope, which makes the photosphere more extended than predictions from 1D models. Massive star lightcurves are always found to vary with a characteristic timescale consistent with the thermal time scale at the location of the iron opacity peak. The amplitude of the variability as well as the power spectrum can explain the commonly observed stochastic low-frequency variability of mass stars observed by TESS over a wide range of parameters in an HR diagram. The 3D simulations can also explain the ubiquitous macro-turbulence that is needed for spectroscopic fitting in massive stars. Implications of 3D simulations for improving 1D stellar evolution models are also discussed.
A Delphi Survey Study to Formulate Statements on the Treatability of Inherited Metabolic Disorders to Decide on Eligibility for Newborn Screening
Authors: Veldman Abigail , Kiewiet M. B. Gea , Westra Dineke , Bosch Annet M. , Brands Marion M. G. , de Coo René I. F. M. , Derks Terry G. J. , Fuchs Sabine A. , van den Hout Johanna. M. P. , Huidekoper Hidde H. , Kluijtmans Leo A. J. , Koop Klaas , Lubout Charlotte M. A. , Mulder Margaretha F. , Panis Bianca , Rubio-Gozalbo M. Estela , de Sain-van der Velden Monique G. , Schaefers Jaqueline , Schreuder Andrea B. , Visser Gepke , Wevers Ron A. , Wijburg Frits A. , Heiner-Fokkema M. Rebecca , van Spronsen Francjan J.
Published: October 2023

The Wilson and Jungner (W&J) and Andermann criteria are meant to help select diseases eligible for population-based screening. With the introduction of next-generation sequencing (NGS) methods for newborn screening (NBS), more inherited metabolic diseases (IMDs) can technically be included, and a revision of the criteria was attempted. This study aimed to formulate statements and investigate whether those statements could elaborate on the criterion of treatability for IMDs to decide on eligibility for NBS. An online Delphi study was started among a panel of Dutch IMD experts (EPs). EPs evaluated, amended, and approved statements on treatability that were subsequently applied to 10 IMDs. After two rounds of Delphi, consensus was reached on 10 statements. Application of these statements selected 5 out of 10 IMDs proposed for this study as eligible for NBS, including 3 IMDs in the current Dutch NBS. The statement: ‘The expected benefit/burden ratio of early treatment is positive and results in a significant health outcome’ contributed most to decision-making. Our Delphi study resulted in 10 statements that can help to decide on eligibility for inclusion in NBS based on treatability, also showing that other criteria could be handled in a comparable way. Validation of the statements is required before these can be applied as guidance to authorities.
A Machine Learning-Based Multiple Imputation Method for the Health and Aging Brain Study–Health Disparities
Authors: Zhang Fan , Petersen Melissa , Johnson Leigh , Hall James , Palmer Raymond F. , O’Bryant Sid E. ,   on behalf of the Health and Aging Brain Study (HABS–HD) Study Team
Published: October 2023

The Health and Aging Brain Study–Health Disparities (HABS–HD) project seeks to understand the biological, social, and environmental factors that impact brain aging among diverse communities. A common issue for HABS–HD is missing data. It is impossible to achieve accurate machine learning (ML) if data contain missing values. Therefore, developing a new imputation methodology has become an urgent task for HABS–HD. The three missing data assumptions, (1) missing completely at random (MCAR), (2) missing at random (MAR), and (3) missing not at random (MNAR), necessitate distinct imputation approaches for each mechanism of missingness. Several popular imputation methods, including listwise deletion, min, mean, predictive mean matching (PMM), classification and regression trees (CART), and missForest, may result in biased outcomes and reduced statistical power when applied to downstream analyses such as testing hypotheses related to clinical variables or utilizing machine learning to predict AD or MCI. Moreover, these commonly used imputation techniques can produce unreliable estimates of missing values if they do not account for the missingness mechanisms or if there is an inconsistency between the imputation method and the missing data mechanism in HABS–HD. Therefore, we proposed a three-step workflow to handle missing data in HABS–HD: (1) missing data evaluation, (2) imputation, and (3) imputation evaluation. First, we explored the missingness in HABS–HD. Then, we developed a machine learning-based multiple imputation method (MLMI) for imputing missing values. We built four ML-based imputation models (support vector machine (SVM), random forest (RF), extreme gradient boosting (XGB), and lasso and elastic-net regularized generalized linear model (GLMNET)) and adapted the four ML-based models to multiple imputations using the simple averaging method. Lastly, we evaluated and compared MLMI with other common methods. Our results showed that the three-step workflow worked well for handling missing values in HABS–HD and the ML-based multiple imputation method outperformed other common methods in terms of prediction performance and change in distribution and correlation. The choice of missing handling methodology has a significant impact on the accompanying statistical analyses of HABS–HD. The conceptual three-step workflow and the ML-based multiple imputation method perform well for our Alzheimer’s disease models. They can also be applied to other disease data analyses.
Rare Case Report: Left Atrial Sarcoma Obstructing the Left Ventricular Inflow
Authors: Kaemmerer Ann-Sophie , Suleiman Mathieu N. , Agaimy Abbas , Harig Frank , Weyand Michael , Tandler René
Published: October 2023

Malignant cardiac tumors of the heart are extremely rare and may present tremendous diagnostic and therapeutic challenges. These tumors are able to infiltrate the heart and metastasize systemically. Early detection is often elusive as the clinical presentation is highly variable, posing significant diagnostic and therapeutic difficulties. Despite a multidisciplinary approach, the prognosis for patients with malignant cardiac tumors remains guarded. Early diagnosis and a multidisciplinary approach involving cardiac surgeons, oncologists and critical care specialists are crucial in the management of this disease. Further research is needed to better understand the pathomechanisms of tumor-related complications and to develop effective treatment strategies to improve patient outcomes. The rare case of a 78-year-old woman with left atrial tumor requiring emergency surgery for acutely developing mitral valve obstruction is presented. Pathology confirmed an undifferentiated pleomorphic sarcoma. This patient tragically did not survive, highlighting the difficulties of managing such a rare and deceptive heart disease.
Identify Optimization Type of Rural Settlements Based on “Production–Living–Ecological” Functions and Vitality: A Case Study of a Town in Northern China
Authors: Zou Yafeng , Yi Chengfeng , Rao Yufei , Luo Feng , Lv Changhe , Wu Pinqi
Published: October 2023

Rural settlements are developing in the direction of functional diversification, driven by rapid urbanization, but also leading to a decline in their vitality as a result of the rapid concentration of rural population in cities. Therefore, this study proposed a theoretical framework to refine the optimization approach for rural settlements from the perspective of “production–living–ecological” functions (PLEF) and vitality. Taking a town in the farming–pastoral ecotone in northern China as a case, we evaluated the level of the PLEF of rural settlements. After exploring the functional requirements of villagers, we revealed the vitality of rural settlements based on social network analysis. The Tapio decoupling model was used to identify the optimization type of rural settlements considering the PLEF and vitality. The results showed that the PLEF of rural settlements was higher in areas with flat terrain, convenient transportation, and rich economies. Rural settlements closer to the central town were stronger in vitality. The PLEF of rural settlements was generally correlated with vitality, which means that rural settlements with a higher level of PLEF also had a stronger vitality. Rural settlements were classified into five types: suburban integration, characteristics protection, agglomeration and upgrading, general survival, relocation, and merger, according to the characteristics of a combination of PLEF and vitality. This study contributes to a deeper comprehension of the functional and structural characteristics of rural settlements and will be beneficial in guiding rural spatial reconstruction.
Molecular Design Concept for Enhancement Charge Carrier Mobility in OFETs: A Review
Authors: Zhou Yang , Zhang Keke , Chen Zhaoyang , Zhang Haichang
Published: October 2023

In the last two decades, organic field-effect transistors (OFETs) have garnered increasing attention from the scientific and industrial communities. The performance of OFETs can be evaluated based on three factors: the charge transport mobility (μ), threshold voltage (Vth), and current on/off ratio (Ion/off). To enhance μ, numerous studies have concentrated on optimizing charge transport within the semiconductor layer. These efforts include: (i) extending π-conjugation, enhancing molecular planarity, and optimizing donor–acceptor structures to improve charge transport within individual molecules; and (ii) promoting strong aggregation, achieving well-ordered structures, and reducing molecular distances to enhance charge transport between molecules. In order to obtain a high charge transport mobility, the charge injection from the electrodes into the semiconductor layer is also important. Since a suitable frontier molecular orbitals’ level could align with the work function of the electrodes, in turn forming an Ohmic contact at the interface. OFETs are classified into p-type (hole transport), n-type (electron transport), and ambipolar-type (both hole and electron transport) based on their charge transport characteristics. As of now, the majority of reported conjugated materials are of the p-type semiconductor category, with research on n-type or ambipolar conjugated materials lagging significantly behind. This review introduces the molecular design concept for enhancing charge carrier mobility, addressing both within the semiconductor layer and charge injection aspects. Additionally, the process of designing or converting the semiconductor type is summarized. Lastly, this review discusses potential trends in evolution and challenges and provides an outlook; the ultimate objective is to outline a theoretical framework for designing high-performance organic semiconductors that can advance the development of OFET applications.
The F/DR-D-10 Algorithm: A Novel Heuristic Strategy to Solve the Minimum Span Frequency Assignment Problem Embedded in Mobile Applications
Authors: Páez-Rueda Carlos-Iván , Fajardo Arturo , Pérez Manuel , Yamhure German , Perilla Gabriel
Published: October 2023

Wireless communication supports various real-world applications, such as aeronautical navigation, satellite and TV broadcasting, wireless LANs, and mobile communications. The inherent characteristics of the electromagnetic spectrum impose constraints on telecommunication channels and their frequency bandwidths within mobile networks. A persistent challenge in these applications is providing high-demand services to mobile users, where frequency assignment problems, also known as channel assignment problems, assume significance. Researchers have developed several modeling approaches to address different facets of this problem, including the management of interfering radio signals, the assessment of available frequencies, and optimization criteria. In this paper, we present improved algorithms for solving the Minimum Span Frequency Assignment Problem in mobile communication systems using the greedy optimization approach known as F/DR. We solved and evaluated twenty well-known benchmark cases to assess the efficacy of our algorithms. Our findings consistently demonstrate that the modified algorithms outperform the F/DR approach with comparable computational complexity. The proposed algorithm notably achieves the following benchmarks: The modified algorithms consistently produce at least one local optimum better than the traditional algorithm in all benchmark tests. In 95% of the benchmarks evaluated, the probability of discovering a local optimum value (calculated by the modified algorithm) that is better than or equal to the one found by the conventional algorithm exceeds 50%.
Event-Triggered Time-Varying Formation Tracking Control for Multi-Agent Systems with a Switching-Directed Topology
Authors: Chen Xiaoya , Chen Huiying
Published: October 2023

This study investigates the problem of time-varying formation tracking (TVFT) control involving event-triggered and switching topological mechanisms. Specifically, TVFT is evaluated with a consensus analysis and deduced via the use of linear matrix inequality techniques combined with Lyapunov stability theory. This strategy obtains sufficient conditions for system stability and the feedback and coupling gains. In addition, the TVFT compensational signals are presented in two cases to enhance the algorithm’s applicability. Given that ideal multi-agent systems (MASs) should be highly flexible and resilient, we propose a co-design algorithm that strikes a balance between the need for a lower communication frequency and a reduction in the state disagreements of agents. Finally, the effectiveness of the theoretical analysis is demonstrated through 3D figures and comparison tables, from which it can be concluded that the communication frequency of the MAS was clearly reduced on the basis of ensuring consensus performance via applying the algorithm proposed in this paper.
Fatigue Crack Propagation on Uniaxial Loading of Biaxially Predeformed Pearlitic Rail Steel
Authors: Gren Daniel , Ahlström Johan
Published: October 2023

Rolling contact fatigue loading causes the surface material in the top of the rail to severely deform. The microstructure aligns along the shear direction and the mechanical behavior in terms of plastic flow becomes anisotropic. Cracks are initiated in the severely deformed surface region and propagates along the direction of microstructure flow lines. However, the effect of large shear deformation on fatigue crack growth is not yet well understood. In this study, uniaxial fatigue crack propagation of undeformed and biaxially predeformed pearlitic rail steel R260 has been investigated. The material was predeformed to different deformation states using combined axial compression and shear deformation, corresponding to material found at different depths in the severely deformed surface layer of rails. Fatigue crack propagation rate was dependent on the material state and influenced by both work hardening and microstructural anisotropy. It was found that predeformed material exhibited lower crack growth rates than undeformed material under this loading condition. The influence of predeformation on the crack growth direction was limited.
Diffusion Welding of Surface Treated Alloy 800H
Authors: Hwang Jong-Bae , Sa Injin , Kim Eung-Seon , Lee Dong-Hyun
Published: October 2023

Diffusion welding of heat-resistant alloys has attracted interest in the manufacturing of components with complex configurations. Controlling secondary precipitates along the interface is necessary to enhance the quality of the diffusion welding. Surface treatment to increase the solubility product (Ksp) of Ti-rich carbide is proposed to accomplish such an enhancement. The reduction of secondary precipitates along the interface induced grain boundary migration across the interface. The chemical compositions at/near the interface satisfied the material specifications. The mechanical properties of the diffusion weldment were similar to those of Alloy 800H that underwent the same thermo-mechanical processes in the range of 25–700 °C. At 25 °C, the tensile strength was 553 MPa, which satisfied the minimum specified tensile strength described in ASTM B: 409-22. The location of failure was random in the gauge section, and dimples, the evidence of the macroscopic plastic deformation, were observed on the fracture surface.
Bacterial Co-Infection in Patients with Coronavirus: A Rapid Review to Support COVID-19 Antimicrobial Prescription
Authors: Celaya Corella María Fernanda , Rodeles Nieblas Jorge Omer , Rechy Iruretagoyena Donato Antonio , Hernández Acevedo Gerson Ney
Published: October 2023

The goal of this study was to determine the most common microorganisms present in COVID-19 patients with co-infections at the General Hospital of Mexicali. Bacterial co-infections have been reported in two previous global pandemics caused by viruses. In a retrospective observational study, we obtained information from 1979 patients. All had symptoms of respiratory disease, and we performed real-time Polymerase Chain Reaction tests on nasopharyngeal swab samples. Of the 1979 patients, 316 were negative; so, they were excluded. One thousand and sixty-three patients had positive results for COVID-19; one hundred and seventy-two (10.34%) had respiratory co-infections. These data were corroborated by positive growth results in culture media and identified using the MALDI-TOF MS System. Vitek 2® Compact, an automated identification system, determined the antimicrobial susceptibility testing results. We analyzed and determined the microorganisms in co-infected patients. Different microorganisms were found, including bacteria and fungi. The most prevalent of all the organisms was Acinetobacter baumannii, which was present in 64 patients (37.2%). We recommend improving the diagnostic and surveillance protocols for possible cases of co-infections in patients with COVID-19. Unlike co-infections in pandemic influenza, the spectrum of microorganisms that cause COVID-19 is too broad and varied to recommend empiric antibiotic therapy.
Epidemiology and Resistance Phenotypes of Carbapenem-Resistant Klebsiella pneumoniae in Corfu General Hospital (2019–2022): A Comprehensive Time Series Analysis of Resistance Gene Dynamics
Authors: Sorovou Glykeria , Schinas Georgios , Pasxali Aggeliki , Tzoukmani Angeliki , Tryfinopoulou Kyriaki , Gogos Charalambos , Dimopoulos George , Akinosoglou Karolina
Published: October 2023

Antimicrobial resistance is a significant global health challenge, with Klebsiella pneumoniae being one of the most common antibiotic-resistant pathogens. This study provides an in-depth analysis of the prevalence and resistance patterns of antibiotic-resistant Klebsiella pneumoniae in the General Hospital of Corfu, Greece, between 2019 and 2022, with the aim of understanding the potential impact of the COVID-19 pandemic on the epidemiology of this bacterium. Utilizing a retrospective epidemiological approach, this study analyzed 212 isolates obtained from the hospital’s Microbiology Department. These isolates were subjected to genotypic and phenotypic identification, with resistance genes (bla-KPC, bla-NDM, bla-VIM, bla-OXA-48, and mcr-1) and antibiotic resistance patterns as the primary focus. The results revealed a significant shift in resistance gene prevalence, with a notable increase in bla-KPC from 16.67% in 2021 to 58.46% in 2022, and a decrease in bla-NDM from 81.48% in 2021 to 38.46% in 2022. In terms of antibiotic resistance patterns, there was a consistent increase in resistance to amikacin and a significant decrease in resistance to ceftazidime/avibactam. These findings underscore the dynamic nature of carbapenem-resistant Klebsiella pneumoniae (CRKP) resistance and highlight the need for ongoing surveillance and adaptive therapeutic strategies in the face of evolving resistance patterns.
Diastereoselective Cascade Cyclization of Diazoimides with Alkylidene Pyrazolones for Preparation of Pyrazole-Fused Oxa-Bridged Oxazocines
Authors: Wang Kuo , Zhang Yue , Cai Lu-Yu , Song Xiu-Qing , Wang Chen , Zhang Jia-Rui , Pan Yi-Fan , Zhao Hong-Wu
Published: October 2023

Under the catalysis of Rh2(OAc)4 (10 mol%) and binapbisphosphine ligand (±)-L3 (20 mol%) in DCE at 80 °C, the cascade cyclization of diazoimides with alkylidenepyrazolones underwent stereoselectively (dr > 20:1), affording pyrazole-fused oxa-bridged oxazocines in reasonable chemical yields. The chemical structure and relative configuration of title products were firmly identified by X-ray diffraction analysis.
Cytotoxic and Antioxidant Potential of Launaea mucronata Forssk Essential Oil Growing in Northern Saudi Arabia
Authors: Mostafa Ehab M. , El-Ghorab Ahmed H. , Ghoneim Mohammed M. , Ebrahim Hasnaa Ali , Abulfaraj Moaz , Abdelgawad Mohamed A. , Farouk Amr , Musa Arafa
Published: October 2023

Essential oils are naturally occurring multicomponent combinations of isoprenoids with distinctive odors that are produced by aromatic plants from mevalonic acid. They are extensively applied in aromatherapy for the treatment of various ailments. To investigate the potential therapeutic value of the ingredients in Launaea mucronata essential oil (EO), gas chromatography–mass spectrometry (GC-MS) analysis was used for essential oil characterization. Then, 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene/linoleic acid, and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays were used to evaluate the antioxidants. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used to estimate the cytotoxicity. Following a thorough analysis of the GC-MS chromatogram, 87 components representing 97.98% of the entire EO mixture were identified. N-eicosane (10.92%), 2E,6Z-farnesol (10.74%), and 2Z,6E-farnesyl acetone (46.35%) were determined to be the major components of the oil. When the produced EO was evaluated for its antioxidant properties, it showed a strong inhibitory effect (%) of 65.34 at a concentration of 80 μg/mL. The results (g/mL) showed a positive response against the tested cell lines for HCT-116, MCF-7, and HepG2 (8.45, 10.24, and 6.78 g/mL, respectively). A high-concentration mixture of deadly components consisting of farnesol, bisabolol, eicosane, and farnesyl acetone may be responsible for this significant cytotoxic action, which was especially noticeable in the HepG2 cell line. Molecular docking occurred between farnesol and farnesyl acetone with the target residues of topoisomerases I and II, CDK4/cyclD1, and Aurora B kinases; these showed binding free energies ranging from −4.5 to −7.4 kcal/mol, thus demonstrating their antiproliferative action. In addition, farnesol and farnesyl acetone fulfilled most of the ADME and drug-likeness properties, indicating their activity.
Thermodynamic Properties of 3- and 4-Ethoxyacetanilides between 80 and 480 K
Authors: Sokolov Andrey A. , Yagofarov Mikhail I. , Balakhontsev Ilya S. , Nizamov Ilyas I. , Mukhametzyanov Timur A. , Solomonov Boris N. , Yurkshtovich Yana N. , Stepurko Elena N.
Published: October 2023

In this work, we present a comprehensive study of the thermodynamic properties of 3-and 4-ethoxyacetanilides. The heat capacities in crystalline, liquid, and supercooled liquid states from 80 to 475 K were obtained using adiabatic, differential scanning (DSC), and fast scanning (FSC) calorimetries. The fusion enthalpies at Tm were combined from DSC measurement results and the literature data. The fusion enthalpies at 298.15 K were evaluated in two independent ways: adjusted according to Kirchhoff’s law of thermochemistry, and using Hess’ law. For the latter approach, the enthalpies of the solution in DMF in crystalline and supercooled liquid states were derived. The values obtained by the two methods are consistent with each other. The standard thermodynamic functions (entropy, enthalpy, and Gibbs energy) between 80 and 470 K were calculated.
A Multifunctional Aggregation-Induced Emission Luminogen with pH-Response Detachable Connector for Lipid Droplet-Specific Imaging and Tracing
Authors: Li Yanjie , Fan Rui , Gao Pengfei , Hu Chang-Hua
Published: October 2023

Lipid droplets (LDs) targeting probes are important for investigating the biological functions of LDs. The interplay between LDs and some other organelles can help to further understand the biological functions of these organelles. However, it is still a challenge to design functional probes that can specifically target LDs and are responsive to some other organelles. Herein, a multifunctional aggregation-induced emission luminogen (AIEgen), namely the TPA-CN, was prepared by the simple aldimine condensation reaction for lipid droplet-specific imaging and tracing. TPA-CN can be sensitively responsive to the acid environment of lysosomes due to the pH-response detachable connector in TPA-CN. With the assistance of this characteristic, it can be concluded from the fluorescence imaging and co-localization analysis results that the internalization of TPA-CN and the targeting of LDs does not involve the lysosome and the lysosomal escape process. At last, the TPA-CN was successfully used for the high-sensitivity imaging of dynamic information of LDs.
Exploring the HIV-1 Rev Recognition Element (RRE)–Rev Inhibitory Capacity and Antiretroviral Action of Benfluron Analogs
Authors: Chumillas Sergi , Loharch Saurabh , Beltrán Manuela , Szewczyk Mateusz P. , Bernal Silvia , Puertas Maria C. , Martinez-Picado Javier , Alcamí José , Bedoya Luis M. , Marchán Vicente , Gallego José
Published: October 2023

Human immunodeficiency virus-type 1 (HIV-1) remains one of the leading contributors to the global burden of disease, and novel antiretroviral agents with alternative mechanisms are needed to cure this infection. Here, we describe an exploratory attempt to optimize the antiretroviral properties of benfluron, a cytostatic agent previously reported to exhibit strong anti-HIV activity likely based on inhibitory actions on virus transcription and Rev-mediated viral RNA export. After obtaining six analogs designed to modify the benzo[c]fluorenone system of the parent molecule, we examined their antiretroviral and toxicity properties together with their capacity to recognize the Rev Recognition Element (RRE) of the virus RNA and inhibit the RRE–Rev interaction. The results indicated that both the benzo[c] and cyclopentanone components of benfluron are required for strong RRE–Rev target engagement and antiretroviral activity and revealed the relative impact of these moieties on RRE affinity, RRE–Rev inhibition, antiviral action and cellular toxicity. These data provide insights into the biological properties of the benzo[c]fluorenone scaffold and contribute to facilitating the design of new anti-HIV agents based on the inhibition of Rev function.
Simultaneous Extraction of Oil and Protein from Silkworm (Bombyx mori L.) Pupae (Lueng Parroj var.) and Their In Vitro Skin Moisturization
Authors: Susirirut Pannarasi , Thitipramote Natthawut , Chaiwut Phanuphong
Published: October 2023

Oil and protein from silkworm (Bombyx mori var. Leung Pairoj) pupae, by-product from sericulture, were extracted and evaluated for their potential uses as skin biomoisturizer. The silkworm pupae (SWP) oil and protein were simultaneously extracted by using three-phase partitioning (TPP) method and determined for their physicochemical properties including fatty acid and amino acid content, respectively. The highest yields of oil and protein at 8.24 ± 0.21% and 8.41 ± 0.26% w/w, respectively were obtained from 18 h extraction. Fatty acid analysis of SWP oil was rich in linolenic acid (37.81 ± 0.34%), oleic acid (28.97 ± 0.13%), palmitic acid (21.27 ± 0.05%), stearic acid (6.60 ± 0.09%) and linoleic acid (4.73 ± 0.21%). The clear yellow SWP oil possessed saponification value of 191.51 mg/g, iodine value of 119.37 g I2/g and peroxide value of 2.00 mg equivalent O2/kg. The SWP protein composed of 17 amino acids which aspartic acid, glutamic acid, glycine and serine were the major residues. SDS-PAGE analysis revealed that the SWP protein consisted of distinct protein at around 51, 70, 175 and over 175 kDa. Cytotoxicity of the SWP oil and protein was evaluated by using MTT assay and they showed low cytotoxicity toward keratinocyte cell (HaCat cell line). The SWP oil provided moisturizing effect on pig skin comparable to olive oil, while 1% and 2% of SWP protein showed higher moisturizing efficacy than 3% hydrolyzed collagen. The study indicated that the SWP oil and protein could be potential biomoisturizers for cosmetic products.
Nurses’ Adherence to the Portuguese Standard to Prevent Catheter-Associated Urinary Tract Infections (CAUTIs): An Observational Study
Authors: Paiva-Santos Filipe , Santos-Costa Paulo , Bastos Celeste , Graveto João
Published: October 2023

Urinary tract infections are among the most prevalent types of healthcare-associated infections (HAIs) in hospitals and nursing homes, and they are primarily a result of unnecessary catheter usage and inadequate care. In Portugal, epidemiological data indicate that catheter-associated urinary tract infections (CAUTIs) remain widespread in clinical settings, resulting in increased morbidity and mortality rates among vulnerable populations. This study aimed to assess urinary catheter use in an oncology ward in Portugal and to evaluate nurses’ adherence to the government-endorsed standards for preventing CAUTIs. An observational study was conducted over a four-month period with daily assessments of nurses’ practices during urinary catheter insertion and maintenance using a government-endorsed auditing tool. Data were collected through on-site observations and nurses’ feedback. The findings revealed a urinary catheter utilization rate of 17.99%. However, there was a lack of complete adherence to government-endorsed standards among oncology nurses (0%). These results indicate that current practices lack evidence-based standardization. Therefore, there is a need to develop and implement quality improvement initiatives to enhance patient safety and experiences.
Comparative Analysis of the Effect of Cutting Piezoelectric Ceramics on Optically Oriented Compensation Capability
Authors: Li Bo , Tian Dapeng
Published: October 2023

In a photoelectric tracking system, a deformable mirror can be used to adjust the transmission path of a light beam to achieve accurate measurement and tracking of the target position. The single-piezoelectric-sheet deformable mirror is a commonly used wavefront correction device with outstanding advantages, such as its simple structure, low cost, and easy preparation. However, it usually has a large cross-linking value and a weak ability to correct higher-order wavefront aberrations. To solve this problem, a novel single-piezoelectric-sheet deformable mirror driving structure is proposed in this paper, in which the coupling between the driving units is weakened by laser cutting the piezoelectric ceramics along the electrode gaps to reduce the cross-linking value of the deformable mirror. In order to verify the effectiveness of this driving structure, this paper takes the 85-unit single-piezoelectric-sheet deformable mirror as an example and simulates the influence function for different cutting depth conditions. The simulation results show that the cross-linking value between neighboring drive units decreases significantly as the piezoelectric-ceramic-laser cutting depth of the drive electrode gap increases. When the piezoelectric ceramic was laser cut to a depth of half of the overall thickness, the cross-linking value decreased by 6.8%.
Broadband Amplification in the 2.6–2.9 μm Wavelength Range in High-Purity Er3+-Doped Zinc-Tellurite Fibers Pumped by Diode Lasers
Authors: Muraviev Sergei , Dorofeev Vitaly , Kuznechikov Pavel , Sharafeev Artem , Koptev Maksim , Kim Arkady
Published: October 2023

In recent years, great progress has been made in the technology of high-purity and ultra-dry tellurite glasses, which has enabled the creation of high-purity single-mode tellurite fibers doped with rare-earth ions. This technology has made it possible to demonstrate laser generation in the range of about 2.7 μm in erbium-doped tungsten tellurite fibers. In this paper, we present an experimental study of broadband amplification in erbium-doped zinc-tellurite fibers. Zinc-tellurite glasses containing modifying components, such as Na2O, La2O3, Bi2O3, or rare-earth metal oxides, are known to have noticeably lower phonon energy than heavy metal-tellurite systems, namely, tungsten tellurite glasses, which leads to better lasing output. The on-off gain of 30- and 60-cm long zinc-tellurite fibers has been measured in a wide range of diode pump powers. It has been shown for the first time that the amplification band is essentially extended, with pump power reaching over 250 nm (2600–2850 nm) at a peak power of about 40 W for a 30-cm long fiber.
Elevated CO2 Can Worsen Fusarium Head Blight Disease Severity in Wheat but the Fhb1 QTL Provides Reliable Disease Resistance
Authors: Hay William T. , Anderson James A. , Garvin David F. , McCormick Susan P. , Busman Mark , Vaughan Martha M.
Published: October 2023

Fusarium head blight (FHB) is a destructive fungal disease of wheat that causes significant economic loss due to lower yields and the contamination of grain with fungal toxins (mycotoxins), particularly deoxynivalenol (DON). FHB disease spread and mycotoxin contamination has been shown to worsen at elevated CO2, therefore, it is important to identify climate-resilient FHB resistance. This work evaluates whether wheat with the Fhb1 quantitative trait locus (QTL), the most widely deployed FHB resistance locus in wheat breeding programs, provides reliable disease resistance at elevated CO2. Near-isogenic wheat lines (NILs) derived from either a highly FHB susceptible or a more FHB resistant genetic background, with or without the Fhb1 QTL, were grown in growth chambers at ambient (400 ppm) and elevated (1000 ppm) CO2 conditions. Wheat was inoculated with Fusarium graminearum and evaluated for FHB severity. At elevated CO2, the NILs derived from more FHB-resistant wheat had increased disease spread, greater pathogen biomass and mycotoxin contamination, and lower rates of DON detoxification; this was not observed in wheat from a FHB susceptible genetic background. The Fhb1 QTL was not associated with increased disease severity in wheat grown at elevated CO2 and provided reliable disease resistance.
Taking on the Habit: Kierkegaardian Faith as an Aristotelian Virtue
Authors: Rojas Fernanda , Bravo Nassim
Published: October 2023

In this article, we would like to argue that the notion of faith, as seen in the anthropology that Kierkegaard presents in works such as The Sickness unto Death or Postscript, among others, shows striking similarities with the Aristotelian ethics of virtue. In a more specific manner, we wish to propose that faith can be interpreted as a virtue in the Aristotelian sense since one can find the following three aspects in it: (1) faith is a state based on habit; (2) faith makes human beings good; and (3) faith makes the human being perform her characteristic activity well. In our view, these features correspond to Aristotle’s definition of virtue: “If this is so in all cases, the virtue of a human being too will be the state that makes a human being good and makes him perform his characteristic activity well”. (Nicomachean Ethics, 1106a).
A 316MP, 120FPS, High Dynamic Range CMOS Image Sensor for Next Generation Immersive Displays
Authors: Agarwal Abhinav , Hansrani Jatin , Bagwell Sam , Rytov Oleksandr , Shah Varun , Ong Kai Ling , Blerkom Daniel Van , Bergey Jonathan , Kumar Neil , Lu Tim , DaSilva Deanan , Graae Michael , Dibble David
Published: October 2023

We present a 2D-stitched, 316MP, 120FPS, high dynamic range CMOS image sensor with 92 CML output ports operating at a cumulative date rate of 515 Gbit/s. The total die size is 9.92 cm × 8.31 cm and the chip is fabricated in a 65 nm, 4 metal BSI process with an overall power consumption of 23 W. A 4.3 µm dual-gain pixel has a high and low conversion gain full well of 6600e- and 41,000e-, respectively, with a total high gain temporal noise of 1.8e- achieving a composite dynamic range of 87 dB.