bluuebunny/crossref_metadata_2025 huggingface dataset:
Therapeutic Angiogenesis by a “Dynamic Duo”: Simultaneous Expression of HGF and VEGF165 by Novel Bicistronic Plasmid Restores Blood Flow in Ischemic Skeletal Muscle
Authors: Slobodkina Ekaterina , Boldyreva Maria , Karagyaur Maxim , Eremichev Roman , Alexandrushkina Natalia , Balabanyan Vadim , Akopyan Zhanna , Parfyonova Yelena , Tkachuk Vsevolod , Makarevich Pavel
Published: December 2020

Therapeutic angiogenesis is a promising strategy for relief of ischemic conditions, and gene delivery was used to stimulate blood vessels’ formation and growth. We have previously shown that intramuscular injection of a mixture containing plasmids encoding vascular endothelial growth factor (VEGF)165 and hepatocyte growth factor (HGF) leads to restoration of blood flow in mouse ischemic limb, and efficacy of combined delivery was superior to each plasmid administered alone. In this work, we evaluated different approaches for co-expression of HGF and VEGF165 genes in a panel of candidate plasmid DNAs (pDNAs) with internal ribosome entry sites (IRESs), a bidirectional promoter or two independent promoters for each gene of interest. Studies in HEK293T culture showed that all plasmids provided synthesis of HGF and VEGF165 proteins and stimulated capillary formation by human umbilical vein endothelial cells (HUVEC), indicating the biological potency of expressed factors. Tests in skeletal muscle explants showed a dramatic difference and most plasmids failed to express HGF and VEGF165 in a significant quantity. However, a bicistronic plasmid with two independent promoters (cytomegalovirus (CMV) for HGF and chicken b-actin (CAG) for VEGF165) provided expression of both grow factors in skeletal muscle at an equimolar ratio. Efficacy tests of bicistronic plasmid were performed in a mouse model of hind limb ischemia. Intramuscular administration of plasmid induced significant restoration of perfusion compared to an empty vector and saline. These findings were supported by increased CD31+ capillary density in animals that received pHGF/VEGF. Overall, our study reports a first-in-class candidate gene therapy drug to deliver two pivotal angiogenic growth factors (HGF and VEGF165) with properties that provide basis for future development of treatment for an unmet medical need—peripheral artery disease and associated limb ischemia.
The Phospholipid Research Center: Current Research in Phospholipids and Their Use in Drug Delivery
Authors: Drescher Simon , van Hoogevest Peter
Published: December 2020

This review summarizes the research on phospholipids and their use for drug delivery related to the Phospholipid Research Center Heidelberg (PRC). The focus is on projects that have been approved by the PRC since 2017 and are currently still ongoing or have recently been completed. The different projects cover all facets of phospholipid research, from basic to applied research, including the use of phospholipids in different administration forms such as liposomes, mixed micelles, emulsions, and extrudates, up to industrial application-oriented research. These projects also include all routes of administration, namely parenteral, oral, and topical. With this review we would like to highlight possible future research directions, including a short introduction into the world of phospholipids.
Tight Junction Modulating Bioprobes for Drug Delivery System to the Brain: A Review
Authors: Tachibana Keisuke , Iwashita Yumi , Wakayama Erika , Nishino Itsuki , Nishikaji Taiki , Kondoh Masuo
Published: December 2020

The blood-brain barrier (BBB), which is composed of endothelial cells, pericytes, astrocytes, and neurons, separates the brain extracellular fluid from the circulating blood, and maintains the homeostasis of the central nervous system (CNS). The BBB endothelial cells have well-developed tight junctions (TJs) and express specific polarized transport systems to tightly control the paracellular movements of solutes, ions, and water. There are two types of TJs: bicellular TJs (bTJs), which is a structure at the contact of two cells, and tricellular TJs (tTJs), which is a structure at the contact of three cells. Claudin-5 and angulin-1 are important components of bTJs and tTJs in the brain, respectively. Here, we review TJ-modulating bioprobes that enable drug delivery to the brain across the BBB, focusing on claudin-5 and angulin-1.
Combining Chemistry and Engineering for Hepatocellular Carcinoma: Nano-Scale and Smaller Therapies
Authors: Stolley Danielle L. , Crouch Anna Colleen , Özkan Aliçan , Seeley Erin H. , Whitley Elizabeth M. , Rylander Marissa Nichole , Cressman Erik N. K.
Published: December 2020

Primary liver cancer, or hepatocellular carcinoma (HCC), is a major worldwide cause of death from carcinoma. Most patients are not candidates for surgery and medical therapies, including new immunotherapies, have not shown major improvements since the modest benefit seen with the introduction of sorafenib over a decade ago. Locoregional therapies for intermediate stage disease are not curative but provide some benefit. However, upon close scrutiny, there is still residual disease in most cases. We review the current status for treatment of intermediate stage disease, summarize the literature on correlative histopathology, and discuss emerging methods at micro-, nano-, and pico-scales to improve therapy. These include transarterial hyperthermia methods and thermoembolization, along with microfluidics model systems and new applications of mass spectrometry imaging for label-free analysis of pharmacokinetics and pharmacodynamics.
Expression of a Hyperthermophilic Cellobiohydrolase in Transgenic Nicotiana tabacum by Protein Storage Vacuole Targeting
Authors: Benedetti Manuel , Vecchi Valeria , Guardini Zeno , Dall’Osto Luca , Bassi Roberto
Published: December 2020

Plant expression of microbial Cell Wall Degrading Enzymes (CWDEs) is a valuable strategy to produce industrial enzymes at affordable cost. Unfortunately, the constitutive expression of CWDEs may affect plant fitness to variable extents, including developmental alterations, sterility and even lethality. In order to explore novel strategies for expressing CWDEs in crops, the cellobiohydrolase CBM3GH5, from the hyperthermophilic bacterium Caldicellulosiruptor saccharolyticus, was constitutively expressed in N. tabacum by targeting the enzyme both to the apoplast and to the protein storage vacuole. The apoplast targeting failed to isolate plants expressing the recombinant enzyme despite a large number of transformants being screened. On the opposite side, the targeting of the cellobiohydrolase to the protein storage vacuole led to several transgenic lines expressing CBM3GH5, with an enzyme yield of up to 0.08 mg g DW−1 (1.67 Units g DW−1) in the mature leaf tissue. The analysis of CBM3GH5 activity revealed that the enzyme accumulated in different plant organs in a developmental-dependent manner, with the highest abundance in mature leaves and roots, followed by seeds, stems and leaf ribs. Notably, both leaves and stems from transgenic plants were characterized by an improved temperature-dependent saccharification profile.
Effects of Arthrospira platensis Extract on Physiology and Berry Traits in Vitis vinifera
Authors: Salvi Linda , Niccolai Alberto , Cataldo Eleonora , Sbraci Sofia , Paoli Francesca , Storchi Paolo , Rodolfi Liliana , Tredici Mario R. , Mattii Giovan Battista
Published: December 2020

Several advantages on physiology, productivity, and grape quality have been reported for grapevine treated with seaweed extracts, but little is known about the importance of cyanobacterial-based biostimulants in viticulture. The purpose of this pioneering work was to analyze the broad-spectrum effects of the Arthrospiraplatensis F&M-C256 extract on Vitis vinifera L. cv. Pinot Nero grown in pots in optimal conditions and under water stress. To evaluate the effects, major physiological parameters of the plants and the quali-quantitative parameters of grape were analyzed. According to the results obtained in this study, ameliorating effects in leaf gas exchanges induced by A. platensis F&M-C256 treatments were detected in both irrigation regimes. Above all, A. platensis F&M-C256 allowed keeping stomata open without negative consequences in water potential in treated vines under water-stress conditions. In terms of berry traits, A. platensis F&M-C256-treated vines presented higher berry weight in comparison with untreated vines in both water regimes and improved berry composition in treated vines subjected to drought. The results of the present study demonstrated an A. platensis-dependent physiological response in case of abiotic stress, which prominently affects grape traits at harvest.
Seed Priming with Endophytic Bacillus subtilis Modulates Physiological Responses of Two Different Triticum aestivum L. Cultivars under Drought Stress
Authors: Lastochkina Oksana , Garshina Darya , Ivanov Sergey , Yuldashev Ruslan , Khafizova Regina , Allagulova Chulpan , Fedorova Kristina , Avalbaev Azamat , Maslennikova Dilara , Bosacchi Massimo
Published: December 2020

The protective effects against drought stress of the endophytic bacterium Bacillus subtilis 10-4 were measured by studying the priming response in two wheat (Triticum aestivum L.)—Ekada70 (E70) and Salavat Yulaev (SY)—lines, tolerant and susceptible to drought, respectively. B. subtilis 10-4 improved germination and growth parameters under normal conditions in both cultivars with the most pronounced effect observed in cv. E70. Under drought conditions, B. subtilis 10-4 significantly ameliorated the negative impact of stress on germination and growth of cv. E70, but had no protective effect on cv. SY. B. subtilis 10-4 induced an increase in the levels of photosynthetic chlorophyll (Chl) a, Chl b, and carotenoids (Car) in the leaves of cv. E70, both under normal and drought conditions. In cv. SY plants, bacterial inoculation decreased the contents of Chl a, Chl b, and Car under normal conditions, but pigment content were almost recovered under drought stress. B. subtilis 10-4 increased water holding capacity (WHC) of cv. E70 (but did not affect this parameter in cv. SY) and prevented the stress-induced decline in WHC in both cultivars. Notably, B. subtilis 10-4 increased endogenous salicylic acid (SA) concentration in both cultivars, especially in cv. E70. Moreover, B. subtilis 10-4 reduced drought-induced endogenous SA accumulation, which was correlated with the influence of endophyte on growth, indicating a possible involvement of endogenous SA in the implementation of B. subtilis-mediated effects in both cultivars. Overall, B. subtilis 10-4 inoculation was found to increase drought tolerance in seedlings of both cultivars, as evidenced by decreased lipid peroxidation, proline content, and electrolyte leakage from tissues of wheat seedlings primed with B. subtilis 10-4 under drought conditions.
The Effects of Postharvest Treatments and Sunlight Exposure on the Reproductive Capability and Viability of Phyllosticta citricarpa in Citrus Black Spot Fruit Lesions
Authors: Moyo Providence , Fourie Paul H. , Masikane Siyethemba L. , de Oliveira Fialho Régis , Mamba Lindokuhle C. , du Plooy Wilma , Hattingh Vaughan
Published: December 2020

Citrus black spot (CBS) is caused by Phyllosticta citricarpa, which is classified as a quarantine organism in certain countries whose concerns are that CBS-infected fruit may be a pathway for introduction of the pathogen. This study evaluated the reproductive capability and viability of P. citricarpa under simulated conditions in which the whole fruit, peel segments, or citrus pulp with CBS lesions were discarded. Naturally infected ‘Midknight’ Valencia orange and ‘Eureka’ lemon fruit, either treated using standard postharvest sanitation, fungicide, and wax coating treatments or untreated, were placed into cold storage for 5 weeks (oranges at 4 °C and lemons at 7 °C). Thereafter, treated and untreated fruit were incubated for a further 2 weeks at conditions conducive for CBS symptom expression and formation of pycnidia. The ability of pycnidia to secrete viable pycnidiospores after whole fruit and peel segments or peel pieces from citrus pulp were exposed to sunlight at warm temperatures (±28 °C) and ±75% relative humidity levels was then investigated. The combination of postharvest treatments and cold storage effectively controlled CBS latent infections (>83.6% control) and pycnidium formation (<1.4% of lesions formed pycnidia), and the wax coating completely inhibited pycnidiospore release in fruit and peel segments. Pycnidiospores were secreted only from lesions on untreated fruit and peel segments and at low levels (4.3–8.6%) from peel pieces from pulped treated fruit. However, spore release rapidly declined when exposed to sunlight conditions (1.4% and 0% after 2 and 3 days, respectively). The generally poor reproductive ability and viability of CBS fruit lesions on harvested fruit, particularly when exposed to sunlight conditions, supports the conclusion that citrus fruit without leaves is not an epidemiologically significant pathway for the entry, establishment, and spread of P. citricarpa.
Chloroplast Distribution in the Stems of 23 Eucalypt Species
Authors: Burrows Geoffrey E. , Connor Celia
Published: December 2020

Small diameter branchlets and smooth barked stems and branches of most woody plants have chloroplasts. While the stems of several eucalypt species have been shown to photosynthesise, the distribution of chloroplasts has not been investigated in detail. The distribution of chloroplasts in branchlets (23 species) and larger diameter stems and branches with smooth bark (14 species) was investigated in a wide range of eucalypts (species of Angophora, Corymbia and Eucalyptus) using fresh hand sections and a combination of bright field and fluorescence microscopy. All species had abundant stem chloroplasts. In both small and large diameter stems, the greatest concentration of chloroplasts was in a narrow band (usually 100–300 μm thick) immediately beneath the epidermis or phellem. Deeper chloroplasts were present but at a lower density due to abundant fibres and sclereids. In general, chloroplasts were found at greater depths in small diameter stems, often being present in the secondary xylem rays and the pith. The cells of the chlorenchyma band were small, rounded and densely packed, and unlike leaf mesophyll. A high density of chloroplasts was found just beneath the phellem of large diameter stems. These trees gave no external indication that green tissues were present just below the phellem. In these species, a thick phellem was not present to protect the inner living bark. Along with the chlorenchyma, the outer bark also had a high density of fibres and sclereids. These sclerenchyma cells probably disrupted a greater abundance and a more organised arrangement of the cells containing chloroplasts. This shows a possible trade-off between photosynthesis and the typical bark functions of protection and mechanical strength.
Polyethylene Glycol 35 (PEG35) Modulates Exosomal Uptake and Function
Authors: Ferrero-Andrés Ana , Closa Daniel , Roselló-Catafau Joan , Folch-Puy Emma
Published: December 2020

Polyethylene glycols (PEGs) are neutral polymers widely used in biomedical applications due to its hydrophilicity and biocompatibility. Exosomes are small vesicles secreted by nearly all cell types and play an important role in normal and pathological conditions. The purpose of this study was to evaluate the role of a 35-kDa molecular weight PEG (PEG35) on the modulation of exosome-mediated inflammation. Human macrophage-like cells THP-1, epithelial BICR-18, and CAPAN-2 cells were exposed to PEG35 prior to incubation with exosomes of different cellular origins. Exosome internalization was evaluated by confocal microscopy and flow cytometry. In another set of experiments, macrophages were treated with increasing concentrations of PEG35 prior to exposure with the appropriate stimuli: lipopolysaccharide, BICR-18-derived exosomes, or exosomes from acute pancreatitis-induced rats. Nuclear Factor Kappa B (NFκB) and Signal transducer and activator of transcription 3 (STAT3) activation and the expression levels of pro-inflammatory Interleukin 1β (IL1β) were determined. PEG35 administration significantly enhanced the internalization of exosomes in both macrophages and epithelial cells. Further, PEG35 ameliorated the inflammatory response induced by acute pancreatitis-derived exosomes by reducing the expression of IL1β and p65 nuclear translocation. Our results revealed that PEG35 promotes the cellular uptake of exosomes and modulates the pro-inflammatory effect of acute pancreatitis-derived vesicles through inhibition of NFκB, thus emphasizing the potential value of PEG35 as an anti-inflammatory agent for biomedical purposes.
A Highly Efficient Ag Nanoparticle-Immobilized Alginate-g-Polyacrylonitrile Hybrid Photocatalyst for the Degradation of Nitrophenols
Authors: Hasan Imran , Shekhar Charu , Alharbi Walaa , Abu Khanjer Maymonah , Khan Rais Ahmad , Alsalme Ali
Published: December 2020

Herein, we report PAN-g-Alg@Ag-based nanocatalysts synthesis via in situ oxidative free-radical polymerization of acrylonitrile (AN) using Alg@Ag nanoparticles (Alg@Ag NPs). Various analytical techniques, including FTIR, XRD, SEM, TEM, UV–Vis, and DSC, were employed to determine bonding interactions and chemical characteristics of the nanocatalyst. The optimized response surface methodology coupled central composite design (RSM–CCD) reaction conditions were a 35-min irradiation time in a 70-mg L−1 2,4-dinitrophenol (DNP) solution at pH of 4.68. Here, DNP degradation was 99.46% at a desirability of 1.00. The pseudo-first-order rate constant (K1) values were 0.047, 0.050, 0.054, 0.056, 0.059, and 0.064 min−1 with associated half-life (t1/2) values of 14.74, 13.86, 12.84, 12.38, 11.74, 10.82, and 10.04 min that corresponded to DNP concentrations of 10, 20, 30, 40, 50, 60, and 70 mg L−1, respectively, in the presence of PAN-g-Alg@Ag (0.03 g). The results indicate that the reaction followed the pseudo-first-order kinetic model with an R2 value of 0.99. The combined absorption properties of PAN and Alg@Ag NPs on copolymerization on the surface contributed more charge density to surface plasmon resonance (SPR) in a way to degrade more and more molecules of DNP together with preventing the recombination of electron and hole pairs within the photocatalytic process.
Crystallization and Thermal Behaviors of Poly(ethylene terephthalate)/Bisphenols Complexes through Melt Post-Polycondensation
Authors: Chen Shichang , Xie Shangdong , Guang Shanshan , Bao Jianna , Zhang Xianming , Chen Wenxing
Published: December 2020

Three kinds of modified poly(ethylene terephthalate) (PET) were prepared by solution blending combined with melt post-polycondensation, using 4,4′-thiodiphenol (TDP), 4,4′-oxydiphenol (ODP) and hydroquinone (HQ) as the bisphenols, respectively. The effects of TDP, ODP and HQ on melt post-polycondensation process and crystallization kinetics, melting behaviors, crystallinity and thermal stability of PET/bisphenols complexes were investigated in detail. Excellent chain growth of PET could be achieved by addition of 1 wt% bisphenols, but intrinsic viscosity of modified PET decreased with further bisphenols content. Intermolecular hydrogen bonding between carbonyl groups of PET and hydroxyl groups of bisphenols were verified by Fourier transform infrared spectroscopy. Compare to pure PET, both the crystallization rate and melting temperatures of PET/bisphenols complexes were reduced obviously, suggesting an impeded crystallization and reduced lamellar thickness. Moreover, the structural difference between TDP, ODP and HQ played an important role on crystallization kinetics. It was proposed that the crystallization rate of TDP modified PET was reduced significantly due to the larger amount of rigid benzene ring and larger polarity than that of PET with ODP or HQ. X-ray diffraction results showed that the crystalline structure of PET did not change from the incorporation of bisphenols, but crystallinity of PET decreased with increasing bisphenols content. Thermal stability of modified PET declined slightly, which was hardly affected by the molecular structure of bisphenols.
Effects of Bromination-Dehydrobromination on the Microstructure of Isotropic Pitch Precursors for Carbon Fibers
Authors: Liang Dingcheng , Liu Deqian , Yang Shuai , Lu Changyu , Xie Qiang , Liu Jinchang
Published: December 2020

In this work, isotropic pitch precursors are synthesized by the bromination-debromination method with ethylene bottom oil (EO) as the raw material and bromine as the initiator for pitch formation and condensation reactions. The aggregation structure, molecular weight distribution, and molecular structure of isotropic pitch precursors are characterized by thermal mechanical analyzer (TMA), MALDI TOF-MS, and 13C NMR, respectively, for revealing the mechanism of synthesis of isotropic pitch precursors. The results show that at low bromine concentrations, polycyclic aromatic hydrocarbons (PAHs) were mainly ordered in cross-linked structures by bromination-debromination through substitution reactions of side chains. The condensed reactivity can be improved by the effect of bromine, meaning that condensation reaction was aggravated by the method of bromination-dehydrobromination. In the presence of excess bromine, the cross-linked stereo structure of PAHs changed to the planar structure of condensed PAHs, which was not conducive to the subsequent spinning and preparation of carbon fibers.
Chitosan–Hydroxyapatite Composite Layers Generated in Radio Frequency Magnetron Sputtering Discharge: From Plasma to Structural and Morphological Analysis of Layers
Authors: Dreghici Dragana Biliana , Butoi Bogdan , Predoi Daniela , Iconaru Simona Liliana , Stoican Ovidiu , Groza Andreea
Published: December 2020

Chitosan–hydroxyapatite composite layers were deposited on Si substrates in radio frequency magnetron sputtering discharges. The plasma parameters calculated from the current–voltage radio frequency-compensated Langmuir probe characteristics indicate a huge difference between the electron temperature in the plasma and at the sample holder. These findings aid in the understanding of the coagulation pattern of hydroxyapatite–chitosan macromolecules on the substrate surface. An increase in the sizes of the spherical-shape grain-like structures formed on the coating surface with the plasma electron number density was observed. The link between the chemical composition of the chitosan–hydroxyapatite composite film and the species sputtered from the target or produced by excitation/ionization mechanisms in the plasma was determined on the basis of X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and residual gas mass spectrometry analysis.
Hindi Adaptation of Centrality of Religiosity Scale
Authors: Dua Devakshi , Scheiblich Herbert , Padhy Susanta Kumar , Grover Sandeep
Published: December 2020

Although religiosity is part and parcel of life of most Indians, no standardized scale is available in local language which can make findings comparable with other countries’. This study aims to present the adaptations required in the Centrality of Religiosity Scale (CRS, CRSi-20) for the Indian population. Additionally, the study aimed to compare the religiosity as assessed by using CRS among healthy subjects and those with first-episode depression. CRS was translated to Hindi by following the methodology suggested by the World Health Organization. During the process of translation, the scale was adapted to suit to the sociocultural milieu of India. The adapted Hindi version of the scale was used in 80 healthy subjects and 80 patients with first-episode depression. During the process of translation, 14 out of 20 items required adaptations to suit the religious practices in India. The adaptation primarily involved elaboration on certain aspects of religious services and practices, keeping in mind the polytheistic religious beliefs in India. When the adapted Hindi version of CRS was used in both the study groups, there was no significant difference between the two groups, in terms of CRS total scores (t = 1.12; p = 0.26). In terms of various domains of CRS, a significantly higher score was observed in the depression group for the ideology domain (t = 2.02; p = 0.04 *), whereas the healthy group had a significantly higher score for the domain of public practice (t = 2.90; p = 0.004 **). Use of CRS in the Indian context requires some adaptations to suit the religious practices. There are minor differences in the religiosity of patients with depression and healthy subjects.
A Fuzzy Guidance System for Rendezvous and Pursuit of Moving Targets
Authors: Alibani Michael , Innocenti Mario , Pollini Lorenzo
Published: December 2020

This article presents the development of a fuzzy guidance system (FGS) for unmanned aerial vehicles capable of pursuing and performing rendezvous with static and mobile targets. The system is designed to allow the vehicle to approach a maneuvering target from a desired direction of arrival and to terminate the rendezvous at a constant distance from the target. In order to perform a rendezvous with a maneuvering target, the desired direction of arrival is adjusted over time to always approach the target from behind, so that the aircraft and target velocity vectors become aligned. The proposed guidance system assumes the presence of an autopilot and uses a set of Takagi–Sugeno fuzzy controllers to generate the orientation and speed references for the velocity and heading control loops, given the relative position and velocity between the aircraft and the target. The FGS treats the target as a mobile waypoint in a 4-D space (position in 2-dimensions, desired crossing heading and speed) and guides the aircraft on suitable trajectories towards the target. Only when the vehicle is close enough to the rendezvous point, the guidance law is complemented with an additional linear controller to manage the terminal formation keeping phase. The capabilities of the proposed rendezvous-FGS are verified in simulation on both maneuvering and non-maneuvering targets. Finally, experimental results using a multi-rotor aerial system are presented for both fixed and accelerating targets.
A Comprehensive Forest Biomass Dataset for the USA Allows Customized Validation of Remotely Sensed Biomass Estimates
Authors: Menlove James , Healey Sean P.
Published: December 2020

There are several new and imminent space-based sensors intended to support mapping of forest structure and biomass. These instruments, along with advancing cloud-based mapping platforms, will soon contribute to a proliferation of biomass maps. One means of differentiating the quality of different maps and estimation strategies will be comparison of results against independent field-based estimates at various scales. The Forest Inventory and Analysis Program of the US Forest Service (FIA) maintains a designed sample of uniformly measured field plots across the conterminous United States. This paper reports production of a map of statistical estimates of mean biomass, created at approximately the finest scale (64,000-hectare hexagons) allowed by FIA’s sample density. This map may be useful for assessing the accuracy of future remotely sensed biomass estimates. Equally important, fine-scale mapping of FIA estimates highlights several ways in which field- and remote sensing-based methods must be aligned to ensure comparability. For example, the biomass in standing dead trees, which may or may not be included in biomass estimates, represents a source of potential discrepancy that FIA shows to be particularly important in the Western US. Likewise, alternative allometric equations (which link measurable tree dimensions such as diameter to difficult-to-measure variables like biomass) strongly impact biomass estimates in ways that can vary over short distances. Potential mismatch in the conditions counted as forests also varies greatly over space. Field-to-map comparisons will ideally minimize these sources of uncertainty by adopting common allometry, carbon pools, and forest definitions. Our national hexagon-level benchmark estimates, provided in Supplementary Files, therefore addresses multiple pools and allometric approaches independently, while providing explicit forest area and uncertainty information. This range of information is intended to allow scientists to minimize potential discrepancies in support of unambiguous validation.
Analysis of the Possibilities of Using Different Resolution Digital Elevation Models in the Study of Microrelief on the Example of Terrain Passability
Authors: Dawid Wojciech , Pokonieczny Krzysztof
Published: December 2020

In this article, we discuss issues concerning the development of detailed passability maps, which are used in the crisis management process and for military purposes. The paper presents the authorial methodology of the automatic generation of these maps with the use of high-resolution digital elevation models (DEMs) acquired from airborne laser scanning (light detection and ranging (LIDAR)) and photogrammetric data obtained from unmanned aerial vehicle (UAV) measurements. The aim of the article is to conduct a detailed comparison of these models in the context of their usage in passability map development. The proposed algorithm of map generation was tested comprehensively in terms of the source of the used spatial data, the resolution, and the types of vehicles moving in terrain. Tests were conducted on areas with a diversified landform, with typical forms of relief that hinder vehicle movement (bluffs and streams). Due to the huge amount of data to be processed, the comprehensive analysis of the possibilities of using DEMs in different configurations of pixel size was executed. This allowed for decreasing the resolution of the model while maintaining the appropriate accuracy properties of the resulting passability map. The obtained results showed insignificant disparities between both sources of used DEMs and demonstrated that using the model with the 2.5 m pixel size did not significantly degrade the accuracy of the passability maps, which has a huge impact on their generation time.
Does Sentinel-1A Backscatter Capture the Spatial Variability in Canopy Gaps of Tropical Agroforests? A Proof-of-Concept in Cocoa Landscapes in Cameroon
Authors: Numbisi Frederick N. , Van Coillie Frieke
Published: December 2020

A reliable estimation and monitoring of tree canopy cover or shade distribution is essential for a sustainable cocoa production via agroforestry systems. Remote sensing (RS) data offer great potential in retrieving and monitoring vegetation status at landscape scales. However, parallel advancements in image processing and analysis are required to appropriately use such data for different targeted applications. This study assessed the potential of Sentinel-1A (S-1A) C-band synthetic aperture radar (SAR) backscatter in estimating canopy cover variability in cocoa agroforestry landscapes. We investigated two landscapes, in Center and South Cameroon, which differ in predominant vegetation: forest-savannah transition and forest landscape, respectively. We estimated canopy cover using in-situ digital hemispherical photographs (DHPs) measures of gap fraction, verified the relationship with SAR backscatter intensity and assessed predictions based on three machine learning approaches: multivariate bootstrap regression, neural networks regression, and random forest regression. Our results showed that about 30% of the variance in canopy gap fraction in the cocoa production landscapes was shared by the used SAR backscatter parameters: a combination of S-1A backscatter intensity, backscatter coefficients, difference, cross ratios, and normalized ratios. Based on the model predictions, the VV (co-polarization) backscatter showed high importance in estimating canopy gap fraction; the VH (cross-polarized) backscatter was less sensitive to the estimated canopy gap. We observed that a combination of different backscatter variables was more reliable at predicting the canopy gap variability in the considered type of vegetation in this study—agroforests. Semi-variogram analysis of canopy gap fraction at the landscape scale revealed higher spatial clustering of canopy gap, based on spatial correlation, at a distance range of 18.95 m in the vegetation transition landscape, compared to a 51.12 m spatial correlation range in the forest landscape. We provide new insight on the spatial variability of canopy gaps in the cocoa landscapes which may be essential for predicting impacts of changing and extreme (drought) weather conditions on farm management and productivity. Our results contribute a proof-of-concept in using current and future SAR images to support management tools or strategies on tree inventorying and decisions regarding incentives for shade tree retention and planting in cocoa landscapes.
New Textural Indicators for Assessing Above-Ground Cotton Biomass Extracted from Optical Imagery Obtained via Unmanned Aerial Vehicle
Authors: Chen Pengfei , Wang Fangyong
Published: December 2020

Although textural information can be used to estimate vegetation biomass, its use for estimating crop biomass is rare, and previous methods lacked a mechanistic explanation for the relationship to biomass. The objective of the present study was to develop mechanistic textural indices for estimating cotton biomass and solving saturation problems at medium and high biomass levels. A nitrogen (N) fertilization experiment was established, and unmanned aerial vehicle optical images and field measured biomass data were obtained during critical cotton growth stages. Based on these data, two textural indices, namely the normalized difference texture index combining contrast and the inverse difference moment of the green band (NBTI (CON, IDM)g) and normalized difference texture index combining entropy and the inverse difference moment of the green band (NBTI (ENT, IDM)g), were proposed by analyzing the mechanism of texture parameters for biomass prediction and the law of texture parameters changing with biomass. These indices were compared with spectral indices commonly used for biomass estimation using independent validation data, such as the normalized difference vegetation index (NDVI). The results showed that the proposed textural indices performed better than the spectral indices with no saturation problems occurring. The combination of spectral and textural indices using a stepwise regression method performed better for biomass estimation than using only spectral or textural indices. This method has considerable potential for improving the accuracy of biomass estimations for the subsequent delineation of precise cotton management zones.
The Forest Line Mapper: A Semi-Automated Tool for Mapping Linear Disturbances in Forests
Authors: Queiroz Gustavo Lopes , McDermid Gregory J. , Rahman Mir Mustafizur , Linke Julia
Published: December 2020

Forest land-use planning and restoration requires effective tools for mapping and attributing linear disturbances such as roads, trails, and asset corridors over large areas. Most existing linear-feature databases are generated by heads-up digitizing. While suitable for cartographic purposes, these datasets often lack the fine spatial details and multiple attributes required for more demanding analytical applications. To address this need, we developed the Forest Line Mapper (FLM), a semi-automated software tool for mapping and attributing linear features using LiDAR-derived canopy height models. Accuracy assessments conducted in the boreal forest of Alberta, Canada showed that the FLM reliably predicts both the center line (polyline) and footprint (extent polygons) of a variety of linear-feature types including roads, pipelines, seismic lines, and power lines. Our analysis showed that FLM outputs were consistently more accurate than publicly available datasets produced by human photo-interpreters, and that the tool can be reliably deployed across large application areas. In addition to accurately delineating linear features, the FLM generates a variety of spatial attributes associated with line geometry and vegetation characteristics from input canopy height data. Our statistical evaluation indicates that spatial attributes generated by the FLM may be useful for studying and classifying linear features based on disturbance type and ground conditions. The FLM is open-source and freely available and is aimed to assist researchers and land managers working in forested environments everywhere.
Spatiotemporal Analysis of Hydrological Variations and Their Impacts on Vegetation in Semiarid Areas from Multiple Satellite Data
Authors: Zhu Yonghua , Luo Pingping , Zhang Sheng , Sun Biao
Published: December 2020

Understanding the spatiotemporal characteristics of hydrological components and their impacts on vegetation are critical for comprehending hydrological, climatological, and ecological processes under environmental change and solving future water management challenges. Innovative methods need to be developed in semiarid areas to analyze the special hydrological factors in the water resource systems of these areas. Gravity Recovery and Climate Experiment (GRACE) and Global Land Data Assimilation System (GLDAS) were applied with the normalized difference vegetation index (NDVI) data in this paper to analyze spatiotemporal changes of hydrological factors in the Xiliaohe River Basin (XRB). The results showed that precipitation (P), evapotranspiration (ET) and temperature (T) had similar seasonal change patterns at rates of 0.05 cm/yr., 0.01 cm/yr. and −0.05 °C/yr., respectively. Total water storage change (TWSC) was consistent with the change trend of soil moisture change (SMC) and showed a fluctuating trend. Groundwater change (GWC) showed a decreasing trend at a rate of −0.43 cm/yr. P and ET had a greater impact on GLDAS data (R = 0.634, P < 0.05 and R = 0.686, P < 0.01, respectively) than on other factors. GWC was more sensitive to changes in T (R = 0.570, P < 0.05). Furthermore, a lag period of 0 to 1 months was observed for the effects of P and ET on TWSC and GLDAS. NDVI showed an upward trend at a rate of 0.001 yr−1 between 2002 and 2014. A spatial distribution of NDVI was heterogeneous in the study area. ET, GLDAS and GWC in growing season limited vegetation growth and were more important than other factors in XRB. The results may contribute to an understanding of the relationships between the hydrological cycle and climate change and provide scientific support for local environmental management.
Increased Low Degree Spherical Harmonic Influences on Polar Ice Sheet Mass Change Derived from GRACE Mission
Authors: Su Xiaoli , Guo Junyi , Shum C. K. , Luo Zhicai , Zhang Yu
Published: December 2020

Replacing estimates of C20 from the Gravity Recovery and Climate Experiment (GRACE) monthly gravity field solutions by those from satellite laser ranging (SLR) data and including degree one terms has become a standard procedure for proper science applications in the satellite gravimetry community. Here, we assess the impact of degree one terms, SLR-based C20 and C30 estimates on GRACE-derived polar ice sheet mass variations. We report that degree one terms recommended for GRACE Release 06 (RL06) data have an impact of 2.5 times more than those for GRACE RL05 data on the mass trend estimates over the Greenland and the Antarctic ice sheets. The latest recommended C20 solutions in GRACE Technical Note 14 (TN14) affect the mass trend estimates of ice sheets in absolute value by more than 50%, as compared to those in TN11 and TN07. The SLR-based C30 replacement has some impact on the Antarctic ice sheet mass variations, mainly depending on the length of the study period. This study emphasizes that reliable solutions of low degree spherical harmonics are crucial for accurately deriving ice sheet mass balance from satellite gravimetry.
Combining Segmentation Network and Nonsubsampled Contourlet Transform for Automatic Marine Raft Aquaculture Area Extraction from Sentinel-1 Images
Authors: Zhang Yi , Wang Chengyi , Ji Yuan , Chen Jingbo , Deng Yupeng , Chen Jing , Jie Yongshi
Published: December 2020

Marine raft aquaculture (MFA) plays an important role in the marine economy and ecosystem. With the characteristics of covering a large area and being sparsely distributed in sea area, MFA monitoring suffers from the low efficiency of field survey and poor data of optical satellite imagery. Synthetic aperture radar (SAR) satellite imagery is currently considered to be an effective data source, while the state-of-the-art methods require manual parameter tuning under the guidance of professional experience. To preclude the limitation, this paper proposes a segmentation network combined with nonsubsampled contourlet transform (NSCT) to extract MFA areas using Sentinel-1 images. The proposed method is highlighted by several improvements based on the feature analysis of MFA. First, the NSCT was applied to enhance the contour and orientation features. Second, multiscale and asymmetric convolutions were introduced to fit the multisize and strip-like features more effectively. Third, both channel and spatial attention modules were adopted in the network architecture to overcome the problems of boundary fuzziness and area incompleteness. Experiments showed that the method can effectively extract marine raft culture areas. Although further research is needed to overcome the problem of interference caused by excessive waves, this paper provides a promising approach for periodical monitoring MFA in a large area with high efficiency and acceptable accuracy.
Unmanned Aerial Systems-Aided Post-Flood Peak Discharge Estimation in Ephemeral Streams
Authors: Andreadakis Emmanouil , Diakakis Michalis , Vassilakis Emmanuel , Deligiannakis Georgios , Antoniadis Antonis , Andriopoulos Petros , Spyrou Nafsika I. , Nikolopoulos Efthymios I.
Published: December 2020

The spatial and temporal scale of flash flood occurrence provides limited opportunities for observations and measurements using conventional monitoring networks, turning the focus to event-based, post-disaster studies. Post-flood surveys exploit field evidence to make indirect discharge estimations, aiming to improve our understanding of hydrological response dynamics under extreme meteorological forcing. However, discharge estimations are associated with demanding fieldwork aiming to record in small timeframes delicate data and data prone-to-be-lost and achieve the desired accuracy in measurements to minimize various uncertainties of the process. In this work, we explore the potential of unmanned aerial systems (UAS) technology, in combination with the Structure for Motion (SfM) and optical granulometry techniques in peak discharge estimations. We compare the results of the UAS-aided discharge estimations to estimates derived from differential Global Navigation Satellite System (d-GNSS) surveys and hydrologic modelling. The application in the catchment of the Soures torrent in Greece, after a catastrophic flood, shows that the UAS-aided method determined peak discharge with accuracy, providing very similar values compared to the ones estimated by the established traditional approach. The technique proved to be particularly effective, providing flexibility in terms of resources and timing, although there are certain limitations to its applicability, related mostly to the optical granulometry as well as the condition of the channel. The application highlighted important advantages and certain weaknesses of these emerging tools in indirect discharge estimations, which we discuss in detail.
Dynamic Divide Migration as a Response to Asymmetric Uplift: An Example from the Zhongtiao Shan, North China
Authors: Su Qi , Wang Xianyan , Lu Huayu , Xie Hong
Published: December 2020

Previous numerical–analytical approaches have suggested that the main range divide prefers to migrate towards the high uplift flank in the asymmetric tectonic uplift pattern. However, natural examples recording these processes and further verifying the numerical simulations results, are still lacking. In this study, the landscape features, and the probable drainage evolution history of the Zhongtiao Shan, a roughly west-east trending, half-horst block on the southernmost tip of the Shanxi Graben System, were investigated through the geomorphic analyses (i.e., slope and steepness distributions, and the Gilbert and χ metrics). The topographic slope and steepness results indicate that the Zhongtiao Shan, controlled by the north Zhongtiao Shan normal fault, experiences asymmetric uplift and erosion patterns, with higher uplift and erosion on the north range. In addition, the Gilbert and χ metrics suggest that the western part of the main divide is currently stable, while the eastern divide is moving southward. According to the drainage divide stability criteria, we suggest that the uplift and erosion, on the fault side, balance each other well on the western part of the range, while on the eastern part, the uplift is outpaced by the erosion. In addition, a dynamic divide migration model in the asymmetric uplift condition is proposed, indicating that the interaction between uplift and erosion controls the migration and/or stability of the main divide. Deducing through this dynamic model, we suggested that the eastern segment of the north Zhongtiaoshan Fault must have experienced higher activities in the geological history, and the western fault may remain its activity along with the mountain relief generation. This gives a case that specific information on asymmetric neotectonic history and landscape evolution in an orogenic mountain can be uncovered by the proposed dynamic model.
Design and Implementation of a Video/Voice Process System for Recognizing Vehicle Parts Based on Artificial Intelligence
Authors: Kim Kapyol , Jeong Incheol , Cho Jinsoo
Published: December 2020

With the recent development of artificial intelligence along with information and communications infrastructure, a new paradigm of online services is being developed. Whereas in the past a service system could only exchange information of the service provider at the request of the user, information can now be provided by automatically analyzing a particular need, even without a direct user request. This also holds for online platforms of used-vehicle sales. In the past, consumers needed to inconveniently determine and classify the quality of information through static data provided by service and information providers. As a result, this service field has been harmful to consumers owing to such problems as false sales, fraud, and exaggerated advertising. Despite significant efforts of platform providers, there are limited human resources for censoring the vast amounts of data uploaded by sellers. Therefore, in this study, an algorithm called YOLOv3+MSSIM Type 2 for automatically censoring the data of used-vehicle sales on an online platform was developed. To this end, an artificial intelligence system that can automatically analyze an object in a vehicle video uploaded by a seller, and an artificial intelligence system that can filter the vehicle-specific terms and profanity from the seller’s video presentation, were also developed. As a result of evaluating the developed system, the average execution speed of the proposed YOLOv3+MSSIM Type 2 algorithm was 78.6 ms faster than that of the pure YOLOv3 algorithm, and the average frame rate per second was improved by 40.22 fps. In addition, the average GPU utilization rate was improved by 23.05%, proving the efficiency.