bluuebunny/crossref_metadata_2025 huggingface dataset:
Microbial Pigments: Major Groups and Industrial Applications
Authors: Barreto João Vitor de Oliveira , Casanova Livia Marques , Junior Athayde Neves , Reis-Mansur Maria Cristina Pinheiro Pereira , Vermelho Alane Beatriz
Published: December 2023

Microbial pigments have many structures and functions with excellent characteristics, such as being biodegradable, non-toxic, and ecologically friendly, constituting an important source of pigments. Industrial production presents a bottleneck in production cost that restricts large-scale commercialization. However, microbial pigments are progressively gaining popularity because of their health advantages. The development of metabolic engineering and cost reduction of the bioprocess using industry by-products opened possibilities for cost and quality improvements in all production phases. We are thus addressing several points related to microbial pigments, including the major classes and structures found, the advantages of use, the biotechnological applications in different industrial sectors, their characteristics, and their impacts on the environment and society.
Geographic and Ecological Diversity of Green Sulfur Bacteria in Hot Spring Mat Communities
Authors: Bedard Donna L. , Van Slyke Greta , Nübel Ulrich , Bateson Mary M. , Brumfield Sue , An Yong Jun , Becraft Eric D. , Wood Jason M. , Thiel Vera , Ward David M.
Published: December 2023

Three strains of thermophilic green sulfur bacteria (GSB) are known; all are from microbial mats in hot springs in Rotorua, New Zealand (NZ) and belong to the species Chlorobaculum tepidum. Here, we describe diverse populations of GSB inhabiting Travel Lodge Spring (TLS) (NZ) and hot springs ranging from 36.1 °C to 51.1 °C in the Republic of the Philippines (PHL) and Yellowstone National Park (YNP), Wyoming, USA. Using targeted amplification and restriction fragment length polymorphism analysis, GSB 16S rRNA sequences were detected in mats in TLS, one PHL site, and three regions of YNP. GSB enrichments from YNP and PHL mats contained small, green, nonmotile rods possessing chlorosomes, chlorobactene, and bacteriochlorophyll c. Partial 16S rRNA gene sequences from YNP, NZ, and PHL mats and enrichments from YNP and PHL samples formed distinct phylogenetic clades, suggesting geographic isolation, and were associated with samples differing in temperature and pH, suggesting adaptations to these parameters. Sequences from enrichments and corresponding mats formed clades that were sometimes distinct, increasing the diversity detected. Sequence differences, monophyly, distribution patterns, and evolutionary simulation modeling support our discovery of at least four new putative moderately thermophilic Chlorobaculum species that grew rapidly at 40 °C to 44 °C.
Mine Productivity Upper Bounds and Truck Dispatch Rules
Authors: Lisboa Adriano Chaves , Castro Felipe Luz Barbosa , de Venâncio Pedro Vinícius Almeida Borges
Published: December 2023

This paper proposes an upper bound for mine productivity (useful for long-term planning) and also a simple truck dispatch rule (useful for short-term operations) that demonstrates how tight the upper bound can be using a simulation. It also proposes a greedy search to approximate the productivity upper bound, which is faster and often exact. Uncertainty is added to the simulation in order to verify how the productivity responds to it. Typically, the productivity’s upper bound is less tight close to its saturation point as a function of the number of trucks, where adding more trucks only increases queues. Furthermore, more uncertainty in the model typically leads to a less tight upper bound. The results conducted using real data from an open pit mine in Brazil show that the gap between the productivity upper bound and the productivity realization using the proposed dispatch rule for a homogeneous fleet can be less than 2%, but it can be as large as 12% near the productivity saturation point without uncertainty. Even though this gap seems to become arbitrarily small as the number of trucks and the simulation horizon increase, the productivity upper bound is never violated, which validates it as an upper bound and induces optimality for the dispatch rule.
Mesoporous-Layered Double Oxide/MCM-41 Composite with Enhanced Catalytic Performance for Cyclopentanone Aldol Condensation
Authors: Yang Jinfan , Shang Ning , Wang Jiachen , Liu Huimin
Published: December 2023

Layered double oxides are widely employed in catalyzing the aldol condensation for producing biofuels, but its selectivity and stability need to be further improved. Herein, a novel MCM-41-supported Mg–Al-layered double oxide (LDO/MCM-41) was prepared via the in situ integration of a sol–gel process and coprecipitation, followed by calcination. This composite was first employed to catalyze the self-condensation of cyclopentanone for producing high-density cycloalkane precursors. LDO/MCM-41 possessed large specific surface area, uniform pore size distribution, abundant medium basic sites and Bronsted acid sites. Compared with the bulk LDO, LDO/MCM-41 exhibited a higher selectivity for C10 and C15 oxygenates at 150 °C (93.4% vs. 84.6%). The selectivity for C15 was especially enhanced on LDO/MCM-41, which was three times greater than that on LDO. The stability test showed that naked LDO with stronger basic strength had a rapid initial activity, while it suffered an obvious deactivation due to its poor carbon balance. LDO/MCM-41 with lower basic strength had an enhanced stability even with a lower initial activity. Under the optimum conditions (50% LDO loading, 170 °C, 7 h), the cyclopentanone conversion on LDO/MCM-41 reached 77.8%, with a 60% yield of C10 and 15.2% yield of C15.
Effects of Different Flotation Agents on the Nucleation and Growth of Potassium Chloride
Authors: Wang Guangle , Bian Xiao , Shang Zeren , Dong Weibing , Zhang Yi , Wu Songgu
Published: December 2023

The flotation agent is an important collector in the production of potassium chloride and is brought into the crystallization stage with the reflux of the mother liquor. Octadecylamine Hydrochloride (ODA), 1-Dodecylamine Hydrochloride (DAH) and Sodium 1-dodecanesulfonate (SDS) were selected to study their effect on the nucleation of potassium chloride. Focused Beam Reflectance Measurement was used to collect the nucleation-induced periods of KCl in the presence of flotation agents at different supersaturations. Then, empirical equations, classical nucleation theory and growth mechanism equations were employed for data analysis. It was found that the presence of flotation agents increased the nucleation sequence m, and m(ODA) > m(SDS) > m(DAH) > m(H2O). In addition, the interfacial energy data obtained using classical nucleation theory suggest that the flotation agents used in our paper promoted the homogeneous nucleation of KCl (reduced from 5.3934 mJ·m−2 to 5.1434 mJ·m−2) and inhibited the heterogeneous nucleation of KCl (increased from 2.8054 mJ·m−2 to 3.6004 mJ·m−2). This investigation also revealed that the growth of potassium chloride was consistent with the 2D nucleation-mediated growth mechanism, and the addition of flotation agent did not change the growth mechanism of potassium chloride. Finally, the particle size distribution results were exactly consistent with the order of nucleation order m. The study of nucleation kinetics and growth mechanisms of different flotation agents on potassium chloride can provide guidance for optimizing the production process of potassium chloride and developing new flotation agents.
Enhancing c-Si Solar Cell Efficiency in the UV Region: Photophysical Insights into the Use of Eu3+ Complexes for Down-Shifting Layer Applications
Authors: Vargas Fabian , Nelson Ronald , Espinoza Dario , Brito Ivan , Sánchez-Muñoz Laura , Alemany Pere , Ortiz Sergio , Ferrada Pablo , Mestra Alifhers , Llanos Jaime
Published: December 2023

[Eu(3DPIQC)3] (where DPIQC = 3-(diphenyl phosphoryl)-1-isoquinolinecarboxylate), a luminescent europium complex with antenna ligands, has been carefully embedded within a polyvinyl butyral (PVB) matrix and the resulting material was used to prepare films used as luminescent down-shifting layers (LDSLs) for crystalline Si-based solar cells. The films were characterized using photoluminescence spectroscopy, atomic force spectroscopy (AFM), UV-Vis spectroscopy, and fluorescence microscopy. The AFM analysis shows films with low surface roughness, while fluorescence microscopy revealed that the Eu complex embedded in PVB assumed a spheroidal configuration, a morphology especially beneficial for optical applications. The so-obtained LDSLs were utilized as energy converters in c-Si solar cells to enhance the utilization of high-energy photons, thereby improving their overall efficiency. The determination of photovoltaic parameters carried out before and after the deposition of the LDSLs on the c-Si cells confirms a positive effect on the efficiency of the cell. The Jsc increases from 121.6 mA/cm2 to 124.9 mA/cm2, and the open circuit voltage (Voc) is found to be unrelated to the complex concentration in the films. The fill factor (FF) remains constant with the Eu concentration. The EQE curves indicate an enhancement in the performance of the photovoltaic cells within the UV region of the spectrum for all coated devices. Electrochemical impedance spectroscopy (EIS) was also carried out in order to analyze the effect of the Eu complex in the charge transfer process of the devices.
Oxidation Treatments Using Hydrogen Peroxide to Convert Paper-Grade Eucalyptus Kraft Pulp into Dissolving-Grade Pulp
Authors: Vera-Loor Andrea , Rigou Panagiota , Mortha Gérard , Marlin Nathalie
Published: December 2023

Converting paper-grade bleached Kraft pulp into dissolving pulp using eco-friendly chemicals on-site at the mill is a challenge for the pulp industry. In this study, two oxidation systems are evaluated: the first one is based on the use of hydrogen peroxide at various levels of alkalinity; the second one investigates the use of sodium periodate followed by hydrogen peroxide to convert aldehydes into carboxyls and enhance their hemicelluloses removal. Our results have shown that when using only peroxide, the removal of hemicelluloses was not sufficient to improve the pulp’s dissolving ability. Conversely, the periodate–peroxide system proved to be more efficient. Results regarding the pulp purity, solubility, degradation (pulp viscosity and cellulose molecular mass distribution), brightness, and its potential applications were discussed.
Antifungal and Phytotoxic Activities of Isolated Compounds from Helietta parvifolia Stems
Authors: Ribeiro Victor Pena , Bajsa-Hirschel Joanna , Tamang Prabin , Meepagala Kumudini , Duke Stephen O.
Published: December 2023

The identification of natural and environmentally friendly pesticides is a key area of interest for the agrochemical industry, with many potentially active compounds being sourced from numerous plant species. In this study, we report the bioassay-guided isolation and identification of phytotoxic and antifungal compounds from the ethyl acetate extract of Helietta parvifolia stems. We identified eight compounds, consisting of two coumarins and six alkaloids. Among these, a new alkaloid, 2-hydroxy-3,6,7-trimethoxyquinoline-4-carbaldehyde (6), was elucidated, along with seven known compounds. The phytotoxicity of purified compounds was evaluated, and chalepin (4) was active against Agrostis stolonifera at 1 mM with 50% inhibition of seed germination and it reduced Lemna pausicotata (duckweed) growth by 50% (IC50) at 168 μM. Additionally, we evaluated the antifungal activity against the fungal plant pathogen Colletotrichum fragariae using a thin-layer chromatography bioautography assay, which revealed that three isolated furoquinoline alkaloids (flindersiamine (3), kokusagenine (7), and maculine (8)) among the isolated compounds had the strongest inhibitory effects on the growth of C. fragariae at all tested concentrations. Our results indicate that these active natural compounds, i.e., (3), (4), (7), and (8), could be scaffolds for the production of more active pesticides with better physicochemical properties.
Application of Sonotriboluminescence to Determine Arene Molecules in Hydrocarbons
Authors: Tukhbatullin Adis A. , Panova Nadezhda A. , Galimov Dim I. , Gareev Bulat M. , Tukhbatullina Alina A. , Vasilyuk Kristina S. , Sharipov Glyus L.
Published: December 2023

The sonotriboluminescence of suspensions of terbium(III) and europium(III) sulfates in decane without and in the presence of benzene, toluene and p-xylene was studied. The choice of crystals of these lanthanides is due to the fact that they have intense luminescence during mechanical action, and also do not dissolve in hydrocarbon solvents. During ultrasonic exposure to suspensions in pure alkanes, bands of Ln3+ ions and N2 in the UV region are recorded in the luminescence spectrum. When aromatic hydrocarbon molecules are added, bands of benzene, toluene and p-xylene molecules, coinciding with their photoluminescence spectra, are recorded in the sonotriboluminescence spectra in the UV region. The high sensitivity of the luminescence of suspensions to arene additives made it possible to obtain the dependence of the characteristic fluorescence of arene molecules in the sonotriboluminescence spectra on their concentration in suspensions. The limits of detection of benzene, toluene and p-xylene in the composition of this suspension were established. The lower limits of detection from the sonotriboluminescence spectra for xylene, toluene and benzene are 0.1, 3 and 50 ppmv, respectively. Fluorescence bands of these molecules were also recorded in the sonotriboluminescence spectra of suspensions in commercial dodecane and heptane with additives of commercial gasoline (up to 1%). The results obtained can be used for luminescent detection of aromatic compounds in saturated hydrocarbons.
Mechanism and Progress of Natural Products in the Treatment of NAFLD-Related Fibrosis
Authors: Li Jin-Zhong , Chen Ning , Ma Nan , Li Min-Ran
Published: December 2023

Nonalcoholic fatty liver disease (NAFLD) has emerged as the most prevalent chronic liver disorder worldwide, with liver fibrosis (LF) serving as a pivotal juncture in NAFLD progression. Natural products have demonstrated substantial antifibrotic properties, ushering in novel avenues for NAFLD treatment. This study provides a comprehensive review of the potential of natural products as antifibrotic agents, including flavonoids, polyphenol compounds, and terpenoids, with specific emphasis on the role of Baicalin in NAFLD-associated fibrosis. Mechanistically, these natural products have exhibited the capacity to target a multitude of signaling pathways, including Hedgehog, Wnt/β-catenin, TGF-β1, and NF-κB. Moreover, they can augment the activities of antioxidant enzymes, inhibit pro-fibrotic factors, and diminish fibrosis markers. In conclusion, this review underscores the considerable potential of natural products in addressing NAFLD-related liver fibrosis through multifaceted mechanisms. Nonetheless, it underscores the imperative need for further clinical investigation to authenticate their effectiveness, offering invaluable insights for future therapeutic advancements in this domain.
Sensitive Immunochromatographic Determination of Salmonella typhimurium in Food Products Using Au@Pt Nanozyme
Authors: Hendrickson Olga D. , Byzova Nadezhda A. , Safenkova Irina V. , Panferov Vasily G. , Dzantiev Boris B. , Zherdev Anatoly V.
Published: December 2023

In this study, we developed a sensitive immunochromatographic analysis (ICA) of the Salmonella typhimurium bacterial pathogen contaminating food products and causing foodborne illness. The ICA of S. typhimurium was performed using Au@Pt nanozyme as a label ensuring both colorimetric detection and catalytic amplification of the analytical signal due to nanozyme peroxidase-mimic properties. The enhanced ICA enabled the detection of S. typhimurium cells with the visual limit of detection (LOD) of 2 × 102 CFU/mL, which outperformed the LOD in the ICA with traditional gold nanoparticles by two orders of magnitude. The assay duration was 15 min. The specificity of the developed assay was tested using cells from various Salmonella species as well as other foodborne pathogens; it was shown that the test system detected only S. typhimurium. The applicability of ICA for the determination of Salmonella in food was confirmed in several samples of milk with different fat content, as well as chicken meat. For these real samples, simple pretreatment procedures were proposed. Recoveries of Salmonella in foodstuffs were from 74.8 to 94.5%. Due to rapidity and sensitivity, the proposed test system is a promising tool for the point-of-care control of the Salmonella contamination of different food products on the whole farm-to-table chain.
Perspectives of Hospitalized Mental Health Care Users Concerning the Involvement of Family Members in Their Care: A Qualitative Study
Authors: Shimange Mihloti E. , Shilubane Hilda N.
Published: December 2023

The value of families and professionals in mental health care is well understood. Patient perspectives appear to have gotten less attention to date. This study investigated the perspectives of hospitalized mental health care users on the involvement of family members in their care using a qualitative phenomenological design. The participants with lived experience of family members being involved in their care were chosen using non-probability, purposive sampling. Individual interviews were carried out with the assistance of a voice recorder and observation notes. Because of data saturation, only fifteen people were interviewed. The interviews were transcribed verbatim and analyzed using Colaizzi’s method. It started with reading and reviewing the transcript to extract key statements about the phenomenon. The meaning was then determined by carefully studying the primary significant statements and phrases. The established meanings were then organized into themes and subthemes. The three themes that emerged from the data analysis are as follows: a wide variation in patients’ perspectives when family members remind them of their medicine, unpredictable visitation by family members, and a lack of support from family members. There were also the following five sub-themes: not visiting mental health care users in the hospital causes uncertainty about their future, mental health care users were concerned by fewer visits from relatives, friends were perceived as a contributory factor to no or limited visitation by family members, disappointment by a lack of financial support, and perceived lack of emotional support from family members. There is a need for family members to visit mental health care users to remove uncertainty about their future. Healthcare providers should listen to mental health care users to identify the challenges they are faced with, and hospital policies tailored to enhancing the involvement of family members should be formulated.
Ocular Manifestations of Human Immunodeficiency Virus Infection in the Combination Antiretroviral Therapy Era
Authors: Yang Mingming , Kamoi Koju , Zong Yuan , Zhang Jing , Zou Yaru , Ohno-Matsui Kyoko
Published: December 2023

Since the introduction of combination antiretroviral therapy (cART) in Japan in 2008, the spectrum of ocular manifestations in patients with human immunodeficiency virus (HIV) has changed. This study, conducted at Tokyo Medical and Dental University Hospital between January 2012 and August 2023, aimed to understand the epidemiology and clinical features of ocular manifestations in patients with HIV during the cART era. Of the 218 patients diagnosed with HIV, 23 (10.55%) exhibited ocular manifestations; all were male, aged 32–73. The most prevalent ocular complication was uveitis (60.67%). Notably, the prevalence of uveitis in this cART era has surged compared to earlier Japanese studies. Our data also suggest a potential direct link between uveitis and HIV, particularly in patients who have not yet undergone cART. However, cytomegalovirus retinitis, another prevalent ocular disease in our study, appeared more strongly associated with patients who commenced cART. Neither ocular condition was significantly correlated with CD4+ T-cell count. Importantly, our observed ocular manifestation prevalence (10.55%) was lower than that in previous studies, emphasizing the potential influence of cART and national healthcare support. These findings provide unique insights into the evolution of ocular manifestations in patients with HIV in Japan amidst cART availability.
Why the SAFE—S Strategy for Trachoma? Are Musca sorbens or Scatophaga stercoraria Really the Culprit?—A Brief Historical Review from an Italian Point of View
Authors: Gallenga Carla Enrica , Maritati Martina , Del Boccio Marco , D’Aloisio Rossella , Conti Pio , Mura Marco , Contini Carlo , Gallenga Pier Enrico
Published: December 2023

The biological history of Chlamydia trachomatis is intertwined with the evolution of the man. Infecting Elemental Bodies (EBs), having penetrated mucosal epithelial cells, wrap themselves in a cloak (ĸλαμις) of glycogen that ensures their obligatory intracellular survival and protects this differentiation into Reticulate Bodies (RBs) that feed on cellular ATP. Multiple chemokines and cytokines are involved under the direction of IL-6 in the florid phase and IL-17A in the scar phase. The WHO has successfully identified the SAFE strategy against trachoma (Surgery, Antibiotics, Facial cleansing, Environment) as the blueprint to eliminate the disease by 2020. Recently, interest has been increasingly focused on changing sexual attitudes in different areas of the world, leaving Musca sorbens, Scatophaga stercoraria, and stepsisters fairly blameless, but extolling the role of Chlamydia trachomatis in apparently “sterile” chronic prostatitis or conjunctivitis or, less frequently, in oropharyngitis and proctitis. The addition of an S (SAFE-S) standing for “sexual behavior” was then proposed to also attract the interest and attention not only of Ophthalmologists and Obstetricians/Gynecologists, Urologists/Andrologists, and the School Authorities for information on the prevention of sexually transmitted diseases, but also of Social Physicians and Pediatricians. This means that sexually transmitted infections should be screened in asymptomatic patients with risky sexual behavior or sexual contact with people diagnosed with a transmitted infection.
Demographic Characteristics of Unvaccinated Asymptomatic and Symptomatic SARS-CoV-2 Cases in Barwon South West, Victoria, Australia
Authors: Tobin-Salzman Shinae C. , Cooper Darcie , McNamara Bridgette J. , Athan Eugene , Bennett Catherine M.
Published: December 2023

We investigated 328 SARS-CoV-2 cases in Barwon South West, Victoria, Australia, in the 2020 pre-vaccination period, comparing infections with symptoms to those that remained asymptomatic. De-identified self-reported data on case characteristics and symptom progression from three sequential questionnaires were examined. Multivariable logistic regression was used to model associations between demographic profiles and symptoms. Asymptomatic infections were more than three times as likely to be seen in ethnic minority groups than the Caucasian population after adjusting for gender and age [OR 3.2, 95% CI 1.5–6.7, p < 0.01] and were more common among cases of Asian background [OR 2.8, 95%CI 1.2–6.4]. Asymptomatic infections were also more common in youth and younger adults, but cases were approximately seven times more likely to be in seniors (≥65 years) compared with those 24 years of age or younger after adjusting for sex and ethnicity [OR 6.9, 95% CI 1.3–35.8]. The overrepresentation of ethnic minority groups among asymptomatic infections is suggestive of genetic haplotype variability by ethnic group, conferring greater cross-protection from other coronaviruses in the initial phase of the COVID-19 pandemic. Replication of this analysis in the post-vaccination era and reassessment of symptom expression according to ethnicity in a community with established vaccine and infection-induced immunity would determine whether this is a sustained association or one confined to the early stages of a pandemic in an immunologically naive population. These findings may, in part, reflect differences in testing patterns by ethnicity and true differences in disease expression, both of which are important to understand in order to inform transmission prevention strategies and tailored risk messaging according to ethnic background.
Surgical Management of Traumatic Meniscus Injuries
Authors: Popper Hannah R. , Fliegel Brian E. , Elliott Dawn M. , Su Alvin W.
Published: December 2023

The menisci increase the contact area of load bearing in the knee and thus disperse the mechanical stress via their circumferential tensile fibers. Traumatic meniscus injuries cause mechanical symptoms in the knee, and are more prevalent amongst younger, more active patients, compared to degenerative tears amongst the elderly population. Traumatic meniscus tears typically result from the load-and-shear mechanism in the knee joint. The treatment depends on the size, location, and pattern of the tear. For non-repairable tears, partial or total meniscal resection decreases its tensile stress and increases joint contact stress, thus potentiating the risk of arthritis. A longitudinal vertical tear pattern at the peripheral third red-red zone leads to higher healing potential after repair. The postoperative rehabilitation protocols after repair range from immediate weight-bearing with no range of motion restrictions to non-weight bearing and delayed mobilization for weeks. Pediatric and adolescent patients may require special considerations due to their activity levels, or distinct pathologies such as a discoid meniscus. Further biomechanical and biologic evidence is needed to guide surgical management, postoperative rehabilitation protocols, and future technology applications for traumatic meniscus injuries.
Generation of a Flat Optical Frequency Comb via a Cascaded Dual-Parallel Mach–Zehnder Modulator and Phase Modulator without Using the Fundamental Tone
Authors: Zhang Shiyu , Wang Zixiong , Zuo Xunhe , Ma Chuang , Jiang Yang , Yu Jinlong
Published: December 2023

Under the conventional scheme to generate an optical frequency comb (OFC) using an electro-optic modulator (EOM), the frequency interval of the OFC is determined via the frequency of the fundamental tone of the radio frequency (RF) driving signals. In this work, we use two harmonics without the fundamental tone to drive two EOMs, where the frequency interval of the generated flat OFC is the frequency of the fundamental tone. The orders of the two harmonics are coprime. Specifically, one harmonic drives the first branch of the dual-parallel Mach–Zehnder modulator (DPMZM) only, and the other harmonic drives the phase modulator (PM). The flatness of the OFC is achieved by adjusting the amplitude and phase of the RF driving harmonics as well as the bias of the EOM. Both a simulation and an experiment were carried out to verify the effectiveness of the proposed scheme. When the second harmonic drives the DPMZM and the third harmonic drives the PM, an 11-comb line OFC is generated, where the flatness of the OFC was 0.63 dB and 0.65 dB under the simulation and experiment, respectively. When the third harmonic drives the DPMZM and the second harmonic drives the PM, a 13-comb line OFC is generated, where the flatness of the OFC was 0.62 dB and 0.95 dB under the simulation and experiment, respectively. We also investigate the performance of the generated OFC when one harmonic drives two branches of the DPMZM and the other harmonic drives the PM. The comparison of the OFCs’ performance demonstrates the effectiveness of the proposed scheme.
Delayed Leaf Senescence Improves Radiation Use Efficiency and Explains Yield Advantage of Large Panicle-Type Hybrid Rice
Authors: Deng Jun , Sheng Tian , Zhong Xuefen , Ye Jiayu , Wang Chunhu , Huang Liying , Tian Xiaohai , Liu Ke , Zhang Yunbo
Published: December 2023

Super hybrid rice with predominantly large panicle types has achieved remarkable success in enhancing crop yield. However, when compared with multi-panicle-type varieties, the yield stability of large panicle-type varieties remains a challenge, and limited information is available on the comparative advantages of multi-panicle types. Consequently, a two-year experiment was conducted to evaluate the grain yield, biomass production, leaf area index (LAI), and radiation use efficiency (RUE) of large panicle-type hybrid rice (Y-liangyou 900, YLY900) and multi-panicle-type hybrid rice (C-liangyouhuazhan, CLYHZ) under three nitrogen (N) treatments (0, 180, 270 kg N ha−1). The effects of increased N fertilization were more pronounced in the large panicle-type varieties. YLY900 outperformed CLYHZ in terms of average yield (6% higher), and its yield advantage was attributed to higher spikelets per panicle (28%). Due to YLY900’s RUE being 9% higher than CLYHZ, it results in a 12% greater accumulation of dry matter than CLYHZ. Furthermore, YLY900 exhibited significant improvements of 16%, 4%, and 14% in specific leaf weight, effective leaf area ratio, and LAI at 20 days after the heading stage (20DAH), respectively, compared with CLYHZ. YLY900 also demonstrated a stronger correlation between rice yield and intercepted photosynthetically active radiation (IPAR) compared with CLYHZ, with R2 values of 0.80 and 0.66, respectively. These findings highlight the superior performance of YLY900, resulting from higher light interception percentage (IP) and IPAR values, which consequently led to enhanced RUE and grain yield. Our research reveals that delayed leaf senescence by increasing LAI at the post-heading stage for large panicle-type hybrid rice, thereby contributing to greater RUE, led to higher biomass production and grain yield.
Susceptibility Evaluation to Fire Blight and Genome-Wide Associations within a Collection of Asturian Apple Accessions
Authors: García-Fernández Belén , Dolcet-Sanjuan Ramon , Micheletti Diego , Antón-Díaz María José , Solsona Cristina , Fernández Mercedes , Abad Xavier , Dapena Enrique
Published: December 2023

Fire blight, caused by Erwinia amylovora, is one of the most devastating apple diseases. The selection of cultivars of low susceptibility and the study of the genetic mechanisms of the disease play important roles in fire blight management. The susceptibility level to fire blight was evaluated in 102 accessions originating from Asturias, a cider-producing region located in the north of Spain with a wide apple germplasm. Evaluations took place under quarantine conditions using artificial inoculations of grafted plants. The results revealed wide variation in susceptibility responses and low-susceptible cultivars were identified. In addition, 91 cultivars were genotyped using the Affymetrix Axiom® Apple 480 K SNP array to conduct genome-wide association studies (GWAS). A statistically significant signal was detected on chromosome 10 using the multi-locus mixed model (MLMM). Two genes were identified as major putative candidate genes: a TIR-NBS-LRR class disease protein and a protein containing a development and cell death (DCD) domain. The outcomes of this study provide a promising source of information, particularly in the context of cider apples, and set a starting point for future genetic and breeding approaches.
OsLEA1b Modulates Starch Biosynthesis at High Temperatures in Rice
Authors: Li Gang , Cao Ruijie , Ma Liuyang , Jiao Guiai , Chen Pengfei , Dong Nannan , Li Xinwei , Duan Yingqing , Li Xiaoxue , Zhu Mingdong , Shao Gaoneng , Sheng Zhonghua , Hu Shikai , Tang Shaoqing , Wei Xiangjin , Yu Yinghong , Hu Peisong
Published: December 2023

High temperatures accelerate the accumulation of storage material in seeds, often leading to defects in grain filling. However, the mechanisms regulating grain filling at high temperatures remain unknown. Here, we want to explore the quality factors influenced by the environment and have identified a LATE EMBROYGENESIS ABUNDANT gene, OsLEA1b, a heat-stress-responsive gene in rice grain filling. OsLEA1b is highly expressed in the endosperm, and its coding protein localizes to the nucleus and cytoplasm. Knock-out mutants of OsLEA1b had abnormal compound starch granules in endosperm cells and chalky endosperm with significantly decreased grain weight and grain number per panicle. The oslea1b mutants exhibited a lower proportion of short starch chains with degrees of polymerization values from 6 to 13 and a higher proportion of chains with degrees from 14 to 48, as well as significantly lower contents of starch, protein, and lipid compared to the wild type. The difference was exacerbated under high temperature conditions. Moreover, OsLEA1b was induced by drought stress. The survival rate of oslea1b mutants decreased significantly under drought stress treatment, with significant increase in ROS levels. These results indicate that OsLEA1b regulates starch biosynthesis and influences rice grain quality, especially under high temperatures. This provides a valuable resource for genetic improvement in rice grain quality.
Cambial Age Influences PCD Gene Expression during Xylem Development and Heartwood Formation
Authors: Moshchenskaya Yulia L. , Galibina Natalia A. , Tarelkina Tatiana V. , Nikerova Ksenia M. , Korzhenevsky Maksim A. , Semenova Ludmila I.
Published: December 2023

Heartwood formation is an important ontogenetic stage in Scots pine (Pinus sylvestris L.). The amount of heartwood determines the proportion of functionally active sapwood in the total trunk biomass as well as the quality of wood. The key criterion for heartwood formation is the death of xylem ray parenchyma cells. Previously, models that described the patterns of heartwood formation, depending on the cambial age, were derived from Scots pine trees of different ages. The cambial age is the number of annual xylem layers at the core sampling site at a certain trunk height. We studied the features of the occurrence of programmed cell death (PCD) processes during the xylem differentiation and heartwood formation of 80-year-old Scots pine trees, depending on the cambial age, under the lingonberry pine forest conditions in the transition area of the northern taiga subzone and tundra. We have shown that the distance from the cambial zone to the heartwood boundary does not change significantly with stem height. As the cambial age increases, the lifespan of the formed xylem ray parenchyma cells increases and the activity of PCD genes decreases during the formation of both (1) xylem (in the outer layers of sapwood) and (2) heartwood (in the inner layers of sapwood and transition zone). We hypothesized that the decisive factor in the PCD initiation during heartwood formation is the distance of the xylem ray parenchyma cells from the cambial zone. The younger cambium forms wider annual increments, and therefore the xylem ray parenchyma cells in these parts of the trunk reach the distance from the cambial zone earlier, which is necessary for PCD initiation.
The Search for Species Flocks in Marine Benthic Homoeocladia spp. (Diatomeae: Bacillariales). I. Variations on Three Themes, Seventeen New Species
Authors: Lobban Christopher S. , Sison Britney , Ashworth Matt P.
Published: December 2023

Exploration of the diversity in the diatom genus Homoeocladia across Micronesia revealed several clusters of undescribed species based on variations around several characters. Using ultrastructural data from scanning electron microscopy, we describe seventeen new species in three of these morphological groups. (1) A group with external thickenings includes eight new species with costae and/or bordered areolae on valve face and/or conopea and/or peri-raphe zone, and one with similar areolae but no ornamentation; this group includes the previously described H. jordanii. (2) Large, linear species, resembling H. asteropeae and H. tarangensis; we describe three new species close to the latter. (3) A sinuous-areolae group includes five new species with areola openings shaped like “~”, “s”, or “z” on the valve and/or girdle bands, or both, and leads to reconsideration of the diagnosis of Homoeocladia schefterae and the recognition that the globally widespread species in this complex is H. coacervata sp. nov. The three groups are based solely on morphology and no genetic relationships are implied within or between the groups, other than having the characteristics of the recently redefined genus Homoeocladia. However, the high diversity of species in Homoeocladia suggests the genus is a good candidate to test for species flocks in this region and in at least one other comparable location, incorporating DNA sampling through either culturing or metabarcoding.
Biochemical and Functional Characterization by Site-Directed Mutagenesis of a Phospholipase A2 from Scorpio maurus Venom
Authors: Krayem Najeh , Alonazi Mona , Khemakhem Bassem , Horchani Habib , Cherif Slim , Karray Aida , Ben Bacha Abir
Published: December 2023

The study of amino acid interactions in the active site of scorpion venom phospholipases A2 could help to gain insights into the structure–function relationship and the biological activities of the enzyme. In the secreted phospholipase A2 of Scorpio maurus venom glands, Glutamate 63 and Tyrosine 122 amino acids play critical roles in the catalytic mechanism through interactions with residues around the calcium-binding loop. We constructed mutants at these positions by overexpression in Escherichia coli cells. After refolding and purification of recombinant enzymes, we studied their kinetic properties using pH-stat and monolayer techniques. The mutant Glutamate 63–Aspartate (E63D) exhibited a reduced activity, while the second mutant Tyrosine 122–Arginine (Y122R) retained some activity with a 14-fold reduction in catalytic efficiency. However, both mutants remained stable in pH values ranging from 2 to 12 whereas the double mutant D63–R122 was catalytically inactive. Comparative analysis of wild-type and mutant 3-D models showed various modifications of the hydrogen-binding network linking residues Glutamate 63 and Tyrosine 122. These modifications of interactions could explain the reduction in enzymatic activity. The kinetic behavior on phosphatidylcholine and phosphatidylethanolamine monolayers of three mutants was evaluated using a baro-stat system to assess the potential association between the hydrolysis of erythrocyte membrane phospholipids and the enzyme’s capability to penetrate phospholipid monolayers at high surface pressure. Mutants’ kinetic behaviors were similar to the wild-type form with slightly modified specific activities at high surface pressure. All mutants were more active on phosphatidylethanolamine than phosphatidylcholine films at high surface pressure. This study provided new information to further elucidate structure–function relationships of scorpion venom-secreted phospholipases A2 and the design of novel potent drug molecules.
Pore Fluid Movability in Fractured Shale Oil Reservoir Based on Nuclear Magnetic Resonance
Authors: Liu Yishan , Chen Zhewei , Ji Dongqi , Peng Yingfeng , Hou Yanan , Lei Zhengdong
Published: December 2023

Gulong shale oil is found in a typical continental shale oil reservoir, which is different from marine shale oil reservoirs. The Gulong shale oil reservoir is a pure shale-type oil reservoir with abundantly developed nanoscale pores, making it extremely difficult to unlock fluids. Pressure drive does not easily achieve fluid unlock conditions; thus, it is necessary to utilize imbibition to unlock nanoscale pore fluids. In this study, experiments were conducted on oil displacement by high-speed centrifugal pressure and imbibition under different conditions, respectively, and simulations were used to evaluate the effects of pressure differential drive and imbibition efficiency on the utilization of crude oil following fracturing. Combined with the mixed wettability of the reservoir, the imbibition efficiency was analyzed, and the imbibition efficiency at different soaking stages was evaluated. When the fracturing pressure was higher than the matrix pore pressure, the imbibition efficiency was the most obvious, which was 27.9%. Spontaneous imbibition depending solely on capillary force had poor efficiency, at 16.8%. When the fracturing pressure was lower than the matrix pore pressure, the imbibition efficiency was the lowest, at only 1.3%. It is proposed that strengthening fracture pressure and promoting pressurized imbibition are the keys to improving shale oil development.
Design of Adhesive Bonded Joints
Authors: Campilho Raul D. S. G.
Published: December 2023

Adhesive bonded joints have become vital to modern engineering, offering advantages such as weight reduction, enhanced fatigue performance, and improved stress distribution [...]
The Impact of Agrivoltaic Systems on Tomato Crop: A Case Study in Southern Italy
Authors: Mohammedi Serine , Dragonetti Giovanna , Admane Naouel , Fouial Abdelouahid
Published: December 2023

Agrivoltaics, a system combining the production of agricultural crops and solar energy on the same land area, offers a potential solution to land use competition between different sectors. However, concerns have been raised regarding the impact of shade on plant growth under Agrivoltaic Systems (AVSs). Numerous studies have explored the effects of AVSs shading on agricultural crops. However, most of these studies focused on shade-tolerant crops, leaving a gap in the understanding of how these systems affect shade-intolerant crops. To this end, this study was conducted in Bari, southern Italy, using two types of AVSs: conventional (Con) and semi-transparent (ST) panels. The objective was to assess the impacts of the different levels of shading on the tomato yield and fruit quality. Tomato cultivation occurred between May and August under various conditions: Con panels, ST panels, and Open Field. The results revealed that soil temperature decreased under both AVSs compared to in the open field conditions. However, the significant reduction in photosynthetically active radiation (PAR), up to 43% in ST and 67% in Con, led to yield reductions ranging between 28% and 58% in ST and Con, respectively. Nonetheless, AVSs demonstrated their potential to reduce irrigation water demand by over 15% in ST and more than 20% in Con. Interestingly, the AVSs reduced fruit size but improved certain fruit quality attributes, such as titratable acidity, which is closely correlated with fruit flavour. These findings highlight the challenges of cultivating shade-intolerant crops under AVSs in a Mediterranean climate, while temperate, dry conditions may offer more favourable prospects for agricultural production.
Quaternary Mammals from Central-Western Argentina in the Stratigraphic Context of Southern South America
Authors: Cerdeño Esperanza , Lucero Natalia P. , Chiesa Jorge O.
Published: December 2023

This is an updated contribution to the Quaternary geology and paleontology from central-western Argentina, focused on San Luis Province. It is mostly based on unpublished data; only some fossils had previously been briefly mentioned in broader faunal contexts. The fossil-bearing sediments correspond to eolian and alluvial environments of moderate energy, dominated by sands and sandy silts. They overlie high-energy fluvial cycles and underlie edaphic horizons. They have a wide distribution, and several radiocarbon dates allow their regional correlation. Stratigraphic sequences with the precise origin of fossils allow for the improvement of lithostratigraphic and faunal correlations with the Pampean Region (central and east Argentina; La Pampa and Buenos Aires provinces), where Pleistocene assemblages are better known, but also with central-western (Cuyo Region), northwestern, and northeastern Argentina. Faunal remains correspond to large mammals, represented by xenarthrans (Cingulata and Tardigrada), macraucheniids (Litopterna), gomphotheres (Proboscidea), and equids (Perissodactyla), a typical Pleistocene mixture of native (xenarthrans and litopterns) and immigrant mammals.
Acerola (Malpighia spp.) Waste: A Sustainable Approach to Nutraceutical, Pharmaceutical, and Energy Applications
Authors: Vega-Baudrit José Roberto , Camacho Melissa , Batista-Menezes Diego , Corrales-Ureña Yendry , Zúñiga Juan Miguel , Chacón Arturo Mora , Lecot Nicole , Henríquez Luis Castillo , Lopretti Mary
Published: December 2023

This study reviews the many uses for waste generated from acerola (Malpighia spp.) production, a tropical fruit renowned for its nutrient-rich content. Traditionally considered an environmental burden, this waste is now gaining attention for its sustainable applications in green technology. This review outlines the extraction of valuable bioactive compounds, like polyphenols, carotenoids, and pectin, that can be extracted from the acerola fruit and acerola waste, and it also delves into its potential in materials science, particularly in the creation of pharmaceutical formulations, nanomaterials, composites, biofuels, and energy applications. On the medical front, the paper highlights the promise that acerola waste holds in anti-inflammatory, antihyperglycemic, and anticancer therapies. By outlining challenges and opportunities, the review emphasizes the untapped potential of acerola waste as a resource for high-value products. These findings suggest a paradigm shift, turning what has been considered waste into a sustainable asset, thereby encouraging environmentally responsible practices within the fruit industry.
Multi-Layer Ceramic Capacitors in Lighting Equipment: Presence and Characterisation of Rare Earth Elements and Precious Metals
Authors: Sideris Konstantinos M. , Fragoulis Dimitrios , Stathopoulos Vassilis N. , Sinioros Panagiotis
Published: December 2023

The need to reduce energy consumption in buildings, the emergence of light-emitting diode (LED) lamps in lighting around 2010, their long lifetime, and the 2025 target to use only LED lamps are changing the existing composition of Category 3 waste electrical–electronic equipment (WEEE) and creating expectations for simple, high-concentration recycling streams. In this study, multi-layer ceramic capacitors (MLCCs) detached from the lighting sector’s WEEE were characterised for the presence of rare earth elements (REEs) and precious metals (PMs). Their digestion was carried out with HNO3 and aqua regia on a heating plate and characterised using inductively coupled plasma optical emission spectroscopy (ICP-OES) and scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX). The contents of REEs and PMs found in the MLCCs were 0.84 wt% and 0.60 wt%, respectively, and create an economic stored value that is essentially defined by PMs of 98.67% and by palladium (Pd) of 78.37%. The analysis showed that the content of the main elements was: neodymium (Nd) 0.366 wt%, yttrium (Y) 0.220 wt%, dysprosium (Dy) 0.131 wt%, silver (Ag) 0.467 wt%, and Pd 0.105 wt%. These results indicate the need for selective removal and separate recycling processes of MLCCs from WEEE drivers.