A proteomics informed by transcriptomics insight into the proteome of Ornithodoros erraticus adult tick saliva
Authors: Pérez-Sánchez Ricardo , Carnero-Morán Angel , Luz Valero M. , Oleaga Ana 
Published: January 2022

Background

The argasid tickOrnithodoros erraticusis the main vector of tick-borne human relapsing fever (TBRF) and African swine fever (ASF) in the Mediterranean Basin. The prevention and control of these diseases would greatly benefit from the elimination ofO. erraticuspopulations, and anti-tick vaccines are envisaged as an effective and sustainable alternative to chemical acaricide usage for tick control.Ornithodoros erraticussaliva contains bioactive proteins that play essential functions in tick feeding and host defence modulation, which may contribute to host infection by tick-borne pathogens. Hence, these proteins could be candidate antigen targets for the development of vaccines aimed at the control and prevention ofO. erraticusinfestations and the diseases this tick transmits. The objective of the present work was to obtain and characterise the proteome of the saliva ofO. erraticusadult ticks as a means to identify and select novel salivary antigen targets.

Methods

A proteomics informed by transcriptomics (PIT) approach was applied to analyse samples of female and male saliva separately using the previously obtainedO. erraticussialotranscriptome as a reference database and two different mass spectrometry techniques, namely liquid chromatography–tandem mass spectrometry (LC–MS/MS) in data-dependent acquisition mode and sequential window acquisition of all theoretical fragment ion spectra MS (SWATH-MS).

Results

Up to 264 and 263 proteins were identified by LC–MS/MS in the saliva ofO. erraticusfemale and male ticks, respectively, totalling 387 non-redundant proteins. Of these, 224 were further quantified by SWATH-MS in the saliva of both male and female ticks. Quantified proteins were classified into 23 functional categories and their abundance compared between sexes. Heme/iron-binding proteins, protease inhibitors, proteases, lipocalins and immune-related proteins were the categories most abundantly expressed in females, while glycolytic enzymes, protease inhibitors and lipocalins were the most abundantly expressed in males. Ninety-seven proteins were differentially expressed between the sexes, of which 37 and 60 were overexpressed in females and males, respectively.

Conclusions

The PIT approach demonstrated its usefulness for proteomics studies ofO. erraticus, a non-model organism without genomic sequences available, allowing the publication of the first comprehensive proteome of the saliva ofO. erraticusreported to date. These findings confirm important quantitative differences between sexes in theO. erraticussaliva proteome, unveil novel salivary proteins and functions at the tick–host feeding interface and improve our understanding of the physiology of feeding inO. erraticusticks. The integration ofO. erraticussialoproteomic and sialotranscriptomic data will drive a more rational selection of salivary candidates as antigen targets for the development of vaccines aimed at the control ofO. erraticusinfestations and the diseases it transmits.

Graphical Abstract