bluuebunny/crossref_metadata_2025 huggingface dataset:
Neuroendocrine Regulation of Seasonal Reproduction
Authors: Tolla Elisabetta , Pérez Jonathan H. , Dunn Ian C. , Meddle Simone L. , Stevenson Tyler J.
Published: July 2019

Neuroendocrine mechanisms control the seasonal reproduction in birds and mammals. Seasonal reproduction is ubiquitous across vertebrate and invertebrate species, and its timing is extremely crucial in order to maximize offspring survival. The hypothalamus is the key brain region that integrates environmental cues. An endogenous circannual timer with oscillations that approximate one year is also localized in the hypothalamus. Successful timing of reproduction involves the combination of endogenous internal timers that are entrained by local environmental cues. Photoperiod, or the annual change in day length, is the primary cue most temperate animals use to predict future environmental conditions. Birds are able to detect light through photoreceptors located in the medio-basal hypothalamus. These photoreceptors are localized in neuroendocrine regions and regulate the key reproductive neuropeptide gonadotropin-releasing hormone (GnRH). In mammals, retinal photoreceptors transduce light information the suprachiasmatic nucleus in the hypothalamus, which then modulates the nocturnal duration of melatonin. Melatonin in mammals is crucial, as it regulates the neuroendocrine release of GnRH and downstream transitions across seasonal reproductive states. The tanycyte cells lining the third ventricle (3rdV) of the hypothalamus are the critical node for the integration of internal (i.e., circannual timing) and external (e.g., photoperiod) information necessary for the regulation of seasonal reproduction.
Teacher Quality in Singapore
Authors: Chong Sylvia , Gopinathan Saravanan
Published: July 2019

Establishing and maintaining teacher quality in Singapore is a process-oriented strategy that requires good policies at the macro level and effective processes at the implementation level. High teacher quality requires rigorous entry requirements, effective evidence-based preparation, and continuous professional development and support at the school level for teacher professionalism. Further adequate compensation and incentives to upskill or reskill are essential. These policies and practices are especially important in this era of challenging pedagogic reform, evolving views of learning and new roles for teachers as learning designers. Teacher policies and practices contribute to the high standing of teachers in Singapore and the consistent high performance of Singapore students in international assessments.
Early Childhood Teacher Education in Global Perspective
Authors: Saracho Olivia N.
Published: July 2019

Teacher educators assume that the teacher education programs in their own countries provide a comprehensive scope of possible selections. Nevertheless, how teacher education is planned and implemented differs in each country. They have different practices in both early childhood education and teacher preparation programs, even though American early childhood education theories and practices have guided them. In addition, countries differ in their early childhood education teacher qualifications. Teacher education programs have been attempting to prepare global-minded early childhood teachers who can function in other countries. Teachers who are prepared with global perspectives are able to help students succeed in the interconnected world where they encounter challenges throughout their lives.

The globalization of early childhood education and the preparation of teachers in the United States and other countries appear ultimately to be achieving importance, respectability, acknowledgment, and wisdom. Several countries have engaged in transforming early childhood teachers through educational reform, which calls upon countries to expand and improve early childhood care and education. Educational reform has intermittently been a main topic of discourse and seldom an emphasis of commitment in countries around the world. Frequently these reform attempts have emphasized the necessity to advance children’s knowledge, abilities, and views to help them become good citizens and productive adults. Whereas developments may not be equivalent from country to country, the movement is continuous. It is encouraging to see countries functioning to advance programs to prepare teachers of young children and to cope with the demanding difficulties and concerns of early childhood education and the preparation of early childhood teachers.
Global Orientations, Local Challenges, and Promises in Initial Teacher Education
Authors: Orland-Barak Lily , Lavrenteva Evgenia
Published: July 2019

The global move toward advanced strategic, constructivist, and sociocultural orientations to student teacher learning is reflected in the stated vision, mission, and curricula of local teacher education contexts worldwide. Six major themes in teacher education programs worldwide are integral to this vision: the establishment of school–community–university partnerships; bringing more of school practice focused on pupil learning into the preparation of future teachers; a shift from a focus on teaching and curriculum to a focus on learning and learners; the inclusion of activities that promote reflective practice and the development of the teacher-as-researcher; the design of academic and school spaces for fostering teacher learning that attends to social justice and inclusion; and the preparation of teacher educators and the provision of mentoring frameworks to support student teacher learning. Among the challenges shared across contexts is the need to strengthen partnerships in education, structure stable mentoring frameworks, adopt a more focused approach to student teacher placement, and better articulate expectations for student teaching. Notwithstanding these challenges, promising directions include the establishment of more meaningful links between universities, schools, and communities; developing programs that deal with authentic teacher preparation through injury- and-research-informed clinical practice, and providing mentoring models that involve different community stakeholders.
Machine Learning in Policy Evaluation: New Tools for Causal Inference
Authors: Kreif Noémi , DiazOrdaz Karla
Published: July 2019

While machine learning (ML) methods have received a lot of attention in recent years, these methods are primarily for prediction. Empirical researchers conducting policy evaluations are, on the other hand, preoccupied with causal problems, trying to answer counterfactual questions: what would have happened in the absence of a policy? Because these counterfactuals can never be directly observed (described as the “fundamental problem of causal inference”) prediction tools from the ML literature cannot be readily used for causal inference. In the last decade, major innovations have taken place incorporating supervised ML tools into estimators for causal parameters such as the average treatment effect (ATE). This holds the promise of attenuating model misspecification issues, and increasing of transparency in model selection. One particularly mature strand of the literature include approaches that incorporate supervised ML approaches in the estimation of the ATE of a binary treatment, under the unconfoundedness and positivity assumptions (also known as exchangeability and overlap assumptions).

This article begins by reviewing popular supervised machine learning algorithms, including trees-based methods and the lasso, as well as ensembles, with a focus on the Super Learner. Then, some specific uses of machine learning for treatment effect estimation are introduced and illustrated, namely (1) to create balance among treated and control groups, (2) to estimate so-called nuisance models (e.g., the propensity score, or conditional expectations of the outcome) in semi-parametric estimators that target causal parameters (e.g., targeted maximum likelihood estimation or the double ML estimator), and (3) the use of machine learning for variable selection in situations with a high number of covariates.

Since there is no universal best estimator, whether parametric or data-adaptive, it is best practice to incorporate a semi-automated approach than can select the models best supported by the observed data, thus attenuating the reliance on subjective choices.
Microstructure of Foreign Exchange Markets
Authors: Evans Martin D. D. , Rime Dagfinn
Published: July 2019

An overview of research on the microstructure of foreign exchange (FX) markets is presented. We begin by summarizing the institutional features of FX trading and describe how they have evolved since the 1980s. We then explain how these features are represented in microstructure models of FX trading. Next, we describe the links between microstructure and traditional macro exchange-rate models and summarize how these links have been explored in recent empirical research. Finally, we provide a microstructure perspective on two recent areas of interest in exchange-rate economics: the behavior of returns on currency portfolios, and questions of competition and regulation.
Boom-Bust Capital Flow Cycles
Authors: Kaminsky Graciela Laura
Published: July 2019

This article examines the new trends in research on capital flows fueled by the 2007–2009 Global Crisis. Previous studies on capital flows focused on current account imbalances and net capital flows. The Global Crisis changed that. The onset of this crisis was preceded by a dramatic increase in gross financial flows while net capital flows remained mostly subdued. The attention in academia zoomed in on gross inflows and outflows with special attention to cross-border banking flows before the crisis erupted and the shift towards corporate bond issuance in its aftermath. The boom and bust in capital flows around the Global Crisis also stimulated a new area of research: capturing the “global factor.” This research adopts two different approaches. The traditional literature on the push–pull factors, which before the crisis was mostly focused on monetary policy in the financial center as the “push factor,” started to explore what other factors contribute to the co-movement of capital flows as well as to amplify the role of monetary policy in the financial center on capital flows. This new research focuses on global banks’ leverage, risk appetite, and global uncertainty. Since the “global factor” is not known, a second branch of the literature has captured this factor indirectly using dynamic common factors extracted from actual capital flows or movements in asset prices.
Chemical and Biological Weapons Policy
Authors: Faith Thomas I.
Published: July 2019

Chemical and biological weapons represent two distinct types of munitions that share some common policy implications. While chemical weapons and biological weapons are different in terms of their development, manufacture, use, and the methods necessary to defend against them, they are commonly united in matters of policy as “weapons of mass destruction,” along with nuclear and radiological weapons. Both chemical and biological weapons have the potential to cause mass casualties, require some technical expertise to produce, and can be employed effectively by both nation states and non-state actors. U.S. policies in the early 20th century were informed by preexisting taboos against poison weapons and the American Expeditionary Forces’ experiences during World War I. The United States promoted restrictions in the use of chemical and biological weapons through World War II, but increased research and development work at the outset of the Cold War. In response to domestic and international pressures during the Vietnam War, the United States drastically curtailed its chemical and biological weapons programs and began supporting international arms control efforts such as the Biological and Toxin Weapons Convention and the Chemical Weapons Convention. U.S. chemical and biological weapons policies significantly influence U.S. policies in the Middle East and the fight against terrorism.
Agriculture and Food Aid in US Policymaking during the Cold War
Authors: Ahlberg Kristin L.
Published: July 2019

In the 20th century, US policymakers often attempted to solve domestic agricultural oversupply problems by extending food aid to foreign recipients. In some instances, the United States donated food in times of natural disasters. In other instances, the United States offered commodities to induce foreign governments to support US foreign policy aims or to spur agricultural modernization. These efforts coalesced during the 1950s with the enactment of Public Law 480, commonly known as the Food for Peace program, which provided for a formal, bureaucratic mechanism for the disbursement of commodities. Throughout the second half of the 20th century, successive presidential administrations continued to deploy commodities in advance of their often disparate foreign policy objectives.
Henry Kissinger and US Foreign Relations
Authors: Schwartz Thomas Alan
Published: July 2019

Henry Kissinger was the most famous and most controversial American diplomat of the second half of the 20th century. Escaping Nazi persecution in the 1930s, serving in the American Army of occupation in Germany after 1945, and then pursuing a successful academic career at Harvard University, Kissinger had already achieved national prominence as a foreign policy analyst and defense intellectual when he was appointed national security adviser by President Richard Nixon in January 1969. Kissinger quickly became the president’s closest adviser on foreign affairs and worked with Nixon to change American foreign policy in response to domestic upheaval caused by the Vietnam War in the late 1960s and early 1970s. Nixon and Kissinger’s initiatives, primarily détente with the Soviet Union, the opening to the People’s Republic of China, and ending American involvement in the Vietnam War, received strong domestic support and helped to bring about Nixon’s re-election landslide in 1972. In the wake of the Watergate scandal, Nixon appointed Kissinger secretary of state in August 1973. As Nixon’s capacity to govern deteriorated, Kissinger assumed all-but presidential powers, even putting American forces on alert during the Yom Kippur war and then engaging in “shuttle diplomacy” in the Middle East, achieving the first-ever agreements between Israel and Egypt and Israel and Syria. Kissinger retained a dominating influence over foreign affairs during the presidency of Gerald Ford, even as he became a lightning rod for critics on both the left and right of the political spectrum. Although out of public office after 1977, Kissinger remained in the public eye as a foreign policy commentator, wrote three volumes of memoirs as well as other substantial books on diplomacy, and created a successful international business-consulting firm. His only governmental positions were as chair of the Commission on Central America in 1983–1984 and a brief moment on the 9/11 Commission in 2002.
Theravāda Buddhism
Authors: Bretfeld Sven
Published: July 2019

Theravāda Buddhism is a neologism denoting a variety of historically connected religious traditions. Today these traditions are predominantly spread in Sri Lanka, Myanmar, Thailand, Laos, Cambodia, and parts of Vietnam. More recent communities in other parts of the world (e. g., Europe, the United States, India, Nepal, Australia) are directly or indirectly connected to the traditions of these countries by migration or adaptation processes. These local varieties do not constitute a homogeneous entity. Nevertheless, the use of a common denominator is justified by their interconnected religious histories and a common stock of liturgical, ritual, exegetical, and narrative traditions.

Prior to the 20th century theravāda (or theriya, theravaṃsa) was understood as a nikāya, an institutionalized monastic lineage primarily defined by a specific system of ritual and legal regulations for monks and nuns (the vinaya). This specific lineage became increasingly associated with Sri Lanka during the first millennium ce, while its Indian origins became obscure. Like many other nikāyas, the Theravādins were not uniform in their doctrinal, ethical, and liturgical orientations. The dynamics of local acculturation and active participation in the translocal networks of the ancient “Buddhist World” produced multiple religious expressions and attitudes within the Theravāda monastic lineage through the centuries. Doctrinally, these could range from an exclusive Pāli-based Śrāvakayāna (“Hīnayāna”) approach to the promotion of so-called Mahāyāna and Vajrayāna texts and practices. A series of royally enforced monastic reforms in the 11th and 12th centuries in Sri Lanka strengthened a certain “conservative” camp of the saṅgha and enforced an exclusivist vision of a Theravāda “orthodoxy,” purified from what this group deemed to be inauthentic adulterations of the Buddha’s teaching. This impulse yielded a powerful—though not unchallenged—impact on the further development of Buddhist monasticism in Sri Lanka and, somewhat later, in Southeast Asia. Ultimately they shaped much of the modern ideas about the characteristics of Theravāda Buddhism.

During the first half of the 20th century the nikāya name Theravāda was detached from its technical monastic meaning and became reinterpreted as a type of Buddhism, idealizing the doctrinal content of the canonical scriptures of this lineage—the so-called Pāli Canon—as a binding belief system for all “Theravāda Buddhists,” monastic or lay. In anachronistic extension of this typology, the compound “Theravāda Buddhism” was further understood as the most ancient, or even “original,” form of the Buddhist “creed.”

The adjective form of Theravāda is Theravādin, which can also be used as a noun to denote a follower of Theravāda.
The History of the São Paulo Biennales
Authors: Gil Thiago
Published: July 2019

The first Bienal de São Paulo occurred in 1951 as an event organized by the Museum of Modern Art of São Paulo. At that moment, the principal objectives of the exhibition were to win a place for São Paulo city in the international artistic circuit and present Brazilian modern art to the world. Due to the artistic direction of intellectuals such as Lourival Gomes Machado, Sérgio Milliet, and Mário Pedrosa, the São Paulo Biennales played a central role in the process of the institutionalization of modern art in Brazil, whether through the organization of special exhibitions dedicated to historical vanguards or expanding the museum’s collection through acquisition prizes. Since 1957, the exhibition has occupied the Ciccillo Matarazzo Pavilion, one of the iconic modernist buildings designed by Oscar Niemeyer for Ibirapuera Park.

In 1962, the exhibition was separated from the museum, following the creation that year of the Fundação Bienal de São Paulo, a private nonprofit institution, which since then has been responsible for the organization of the Biennales. During the following decades the history of the Biennales has been a constant effort to survive numerous crises, maintaining a contemporary identity for the exhibition through experimentation with different organizational structures. The exhibition followed the model of the Venice Biennale, based on the geopolitical logic of national representation until 2006, when the Fundação Bienal decided to implement the current organizational system in which an appointed general curator is entirely responsible for the choice of artists for the exhibition. The capacity to reinvent itself from time to time, to adapt to changes in artistic practices and the global artistic scene, is what still makes the São Paulo Biennale the oldest and most important contemporary international art exhibition in Latin America.
The New Philology and the New History of Religion in New Spain
Authors: Christensen Mark
Published: July 2019

The New Philology and its emphasis on the use of indigenous-language sources for ethnohistorical insights contributes greatly to the study of religion in New Spain. Previous studies primarily employed Spanish-language accounts and reports to understand evangelization efforts. Although providing important insights, histories based solely on Spanish sources are limited in their contributions. The New Philology, however, provides an additional point of view from which to study religion. Indigenous-language texts in Nahuatl (Aztec), Yucatec Maya, Mixtec, Zapotec, and other languages contain a wealth of information on how natives responded, negotiated, resisted, and participated in the spread of Catholicism. The contributions of the New Philology to the study of religion in New Spain, although many, are particularly evident in its re-evaluation of the spiritual conquest; the natives’ role in evangelization; the diversity of religious beliefs, practices, and experiences throughout the colonial period; and through its critical study of the legend surrounding the Virgin of Guadalupe.
National History Museum, Chapultepec Castle
Authors: Smithers Salvador Rueda
Published: July 2019

As a monument and museum, Chapultepec Castle is today an emblem for Mexicans. It signified a double synthesis of memory: the building tells the history of the old Military College and the residence of Mexican rulers before the building became a museum. Its interior spaces, the objects of its collection, and the 20th-century murals recount and construct the shared history of Mexicans over the last 500 years.
Phonetics of Consonants
Authors: Fuchs Susanne , Birkholz Peter
Published: July 2019

Consonants are a major class of sounds occurring in all human languages. Typologically, consonant inventories are richer than vowel inventories. Consonants have been classified according to four basic features. Airstream mechanism is one of these features and describes the direction of airflow in or out of the oral cavity. The outgoing airflow is further separated according to its origin, that is, air coming from the lungs (pulmonic) or the oral cavity (non-pulmonic). Consonants are also grouped according to their phonological voicing contrast, which can be manifested phonetically by the presence or absence of vocal fold oscillations during the oral closure/constriction phase and by the duration from an oral closure release to the onset of voicing. Place of articulation is the third feature and refers to the location at which a consonantal constriction or closure is produced in the vocal tract. Finally, manner of articulation reflects different timing and coordinated actions of the articulators closely tied to aerodynamic properties.
Quantitative Methods in Morphology: Corpora and Other “Big Data” Approaches
Authors: Marelli Marco
Published: July 2019

Corpora are an all-important resource in linguistics, as they constitute the primary source for large-scale examples of language usage. This has been even more evident in recent years, with the increasing availability of texts in digital format leading more and more corpus linguistics toward a “big data” approach. As a consequence, the quantitative methods adopted in the field are becoming more sophisticated and various.

When it comes to morphology, corpora represent a primary source of evidence to describe morpheme usage, and in particular how often a particular morphological pattern is attested in a given language. There is hence a tight relation between corpus linguistics and the study of morphology and the lexicon. This relation, however, can be considered bi-directional. On the one hand, corpora are used as a source of evidence to develop metrics and train computational models of morphology: by means of corpus data it is possible to quantitatively characterize morphological notions such as productivity, and corpus data are fed to computational models to capture morphological phenomena at different levels of description. On the other hand, morphology has also been applied as an organization principle to corpora. Annotations of linguistic data often adopt morphological notions as guidelines. The resulting information, either obtained from human annotators or relying on automatic systems, makes corpora easier to analyze and more convenient to use in a number of applications.
ACTINN: automated identification of cell types in single cell RNA sequencing
Authors: Ma Feiyang , Pellegrini Matteo
Published: July 2019

Motivation

Cell type identification is one of the major goals in single cell RNA sequencing (scRNA-seq). Current methods for assigning cell types typically involve the use of unsupervised clustering, the identification of signature genes in each cluster, followed by a manual lookup of these genes in the literature and databases to assign cell types. However, there are several limitations associated with these approaches, such as unwanted sources of variation that influence clustering and a lack of canonical markers for certain cell types. Here, we present ACTINN (Automated Cell Type Identification using Neural Networks), which employs a neural network with three hidden layers, trains on datasets with predefined cell types and predicts cell types for other datasets based on the trained parameters.

Results

We trained the neural network on a mouse cell type atlas (Tabula Muris Atlas) and a human immune cell dataset, and used it to predict cell types for mouse leukocytes, human PBMCs and human T cell sub types. The results showed that our neural network is fast and accurate, and should therefore be a useful tool to complement existing scRNA-seq pipelines.

Availability and implementation

The codes and datasets are available at https://figshare.com/articles/ACTINN/8967116. Tutorial is available at https://github.com/mafeiyang/ACTINN. All codes are implemented in python.

Supplementary information

Supplementary data are available at Bioinformatics online.
Kidney Mentoring and Assessment Program for Students: a guide for engaging medical students in nephrology
Authors: Bayliss George P , Cobb Jason , Decker Brian , Hellman Richard , Vasavada Nina , Mackelaite Lina , Shadur Craig , Ilori Titilayo , Ibrahim Tod , Leight Katlyn , Hsiao Li-Li , Molitoris Bruce A , Okusa Mark D , Parker Mark G ,   ASN Workforce Committee
Published: July 2019

Background

The American Society of Nephrology’s (ASN) Workforce Committee created a unique program called the Kidney Mentoring and Awareness Program for Students to engage medical students in the fight against kidney diseases and interest them in careers in nephrology.

Methods

The program provided a framework and 2 years of funding to three medical schools to organize and carry out health screenings in underserved areas of their communities as well as a structure for student mentoring by the practicing nephrologists.

Results

The Workforce Committee identified three medical schools (Emory University, Atlanta, GA; Indiana University, Indianapolis, IN and University of Louisville, Louisville, KY) and engaged faculty at each school to serve as advisors. The ASN committed funding to the groups for 2 years, after which the groups became self-sufficient. Three nephrologists participated in each chapter, building on existing relationships with community groups to identify sites and carry out kidney screening events.

Conclusions

We report here the experience of those chapters and a blueprint for other schools interested in setting up a similarly structured program to interest students in nephrology while working with community groups to spread awareness of the major underlying causes of kidney disease.
The security implications of fisheries
Authors: DeSombre Elizabeth R.
Published: July 2019

Although frequently ignored in discussion of ocean security, fisheries have had central security implications throughout history. This article re-centres fisheries issues as both a cause and effect of security conflicts, and examines the implications of this re-framing for addressing this intersection. Underlying the security concerns that arise related to fisheries is depletion of global fish stocks. When stocks are overfished or not well managed, fishing vessels move to other areas, where they are more likely to come into conflict with each other and to threaten vulnerable stocks that some populations rely on, and states will claim or defend more ocean territory. These issues are explored here with four sets of security crises that can be best understood by examining the underlying or contributing aspect of fishery depletion: conflicts over sovereignty of small maritime islands, the rise of Somali maritime piracy, the ‘fish wars’ between otherwise friendly states in the middle and latter parts of the twentieth century, and the human insecurity represented by slavery-like conditions aboard some fishing vessels. Understanding the security implications of fisheries grants new reason and new approaches—ideally multi-jurisdictional, transnational and focused on capacity-building—to better protect fisheries and prevent security threats.
Black Soldier Fly Larvae Rearrange under Compression
Authors: Shishkov Olga , Trebuchon Joshua , Yunker Peter J , Franklin Scott , Hu David L
Published: July 2019

Thousands of black soldier larvae hatch simultaneously from eggs laid within rotting vegetation or animal carcasses. Over the next few weeks, they grow while compressed by both their surroundings and each other. When compressed, these larvae rearrange to reduce the forces upon them. How quickly can larvae rearrange, and what final state do they choose? In this experimental study, we use a universal testing machine to conduct creep tests on larvae, squeezing them to set volume fractions and measuring the time course of their reaction force. Live larvae come to equilibrium at a rate 10 times faster than dead larvae, indicating that their small movements can rearrange them faster than just settling. The relaxation of dead larvae is well described by stretched exponentials, which also characterize hierarchical self-avoiding materials such as polymers or balls of crumpled aluminum foil. The equilibrium pressures of live larvae are comparable to those of dead larvae, suggesting that such pressures are dictated by the physics of their bodies rather than their behavior. Live larvae perform fluctuations to actively maintain this equilibrium pressure. This ability to survive large pressures might have applications in the larvae-rearing industry, where both live and dead larvae are packed in containers for shipping.
Authentic Science with Dissemination Increases Self-Efficacy of Middle School Students
Authors: Broder E Dale , Guilbert Katie E , Tinghitella Robin M , Murphy Shannon M , Ghalambor Cameron K , Angeloni Lisa M
Published: July 2019

Ethnically and gender diverse groups are more efficient, creative, and productive than homogeneous groups, yet women and minorities are underrepresented in the science, technology, engineering, and mathematics (STEM) workforce. One contributor is unequal access to high-quality STEM education based on socioeconomic status and race, which we may be able to address through inquiry-based out-of-school time programs. Here we describe a 6-month after-school program that allows an underrepresented community of middle school students to conduct original scientific research that they present at a conference each year. Through qualitative assessments and interviews, we found a trend for increased interest in STEM careers and self-efficacy in participants. Self-efficacy, or belief in one’s ability to succeed, predicts performance and persistence in STEM. Both self-efficacy and interest in STEM careers increased after students presented their research at a conference, highlighting the unexplored importance of dissemination for shaping self-efficacy in K-12 students. Small after-school programs like ours can be easily accomplished as broader impacts by scientists, and well-designed programs have the potential to positively affect change by increasing access and participation in STEM for diverse students.
Tissue residue depletion and estimation of extralabel meat withdrawal intervals for tulathromycin in calves after pneumatic dart administration
Authors: Lin Zhoumeng , He Chunla , Magstadt Drew R , Cooper Vickie L , Kleinhenz Michael D , Smith Joseph S , Gorden Patrick J , Wulf Larry W , Coetzee Johann F
Published: July 2019

The objectives of this study were to evaluate the injection site pathology and determine tissue residue depletion of tulathromycin in calves following pneumatic dart administration and to calculate the associated extralabel withdrawal interval (WDI). Castrated male Holstein calves were injected with ~2.6 mg/kg tulathromycin via pneumatic dart administration. At 1 (n = 2), 6, 12, 18, and 24 d after drug injection (n = 3/time point), calves were euthanized, and muscle, liver, kidney, fat, and injection site samples were harvested and analyzed for tulathromycin concentrations using a LC-MS/MS method. Gross pathology and histopathology evaluations on the injection site samples were also performed. Pneumatic dart administration of tulathromycin caused severe localized lesions of hemorrhage and edema on days 1 and 6, as well as severe pathological reactions in the subcutaneous muscle on days 1, 6, and 12. Slight to moderate reactions were still observed in the majority of the skin or subcutaneous/muscle samples on day 24. Measured tulathromycin concentrations were converted to calculate the concentrations of the marker residue CP-60,300 by dividing a conversion factor of 1.4. The data were used to calculate extralabel WDIs based on the guidelines from U.S. Food and Drug Administration (FDA) and the European Medicines Agency (EMA). The results showed that tulathromycin concentrations were the highest in the liver (4,877.84 ± 65.33 µg/kg), kidney (5,819.52 ± 1,087.00 µg/kg), muscle (1,717.04 ± 140.35 µg/kg), injection site (51,884.05 ± 7,529.34 µg/kg), and fat (161.69 ± 36.48 µg/kg) at 6, 1, 1, 1, and 1 d, respectively, after treatment. Tulathromycin concentrations remained above the limit of quantification of 5 µg/kg in all tissues at 24 d. The calculated WDIs based on kidney data were 26 d using EMA method, 36 d using FDA method based on CP-60,300 data, and 45 d using FDA method based on tulathromycin data. These results suggest that pneumatic dart administration of tulathromycin causes injection site reactions in calves and an extended WDI is needed. One limitation of this study was the small sample size of 3 that did not meet FDA guideline requirement. Therefore, the calculated WDIs should be considered as preliminary and additional studies that use a larger number of animals and directly measure the concentrations of the marker residue CP-60,300 are needed to make a more conclusive recommendation on the extralabel WDI.
Deux Collections De Sentences Coptes Inédites : Paroles D’Instruction D’Athanase ET Paroles Anonymes
Authors: Crégheur Eric
Published: July 2019

This essay presents an edition and translation of two hitherto unedited Coptic collections of sayings preserved in the Royal Ontario Museum (Toronto, Canada), Words of Instruction attributed to Athanasius of Alexandria and a group of anonymous Sayings, once wrongly attributed to Evagrius. After a description of the manuscript (characteristics, provenance, context of its acquisition) and of its contents (texts, language, previous research), I present in greater detail the two previously unedited collections of sayings found in it (characteristics of the sayings, original language, milieu of composition, attribution, dating, etc.). Finally, I offer brief comments on their place among similar collections. The edition and French translation of the two collections follow, each accompanied by notes.
Contracting on Credit Ratings: Adding Value to Public Information
Authors: Parlour Christine A , Rajan Uday
Published: July 2019

We consider the role of credit ratings when contracts between investors and portfolio managers are incomplete. In our model, a credit rating and a price on a risky bond both provide verifiable signals about a non-contractible state. We allow the investor to both impose ex ante restrictions on the manager’s action and provide outcome-based compensation. The optimal contract is a prohibitive one when the rating and price indicate a high likelihood of a low state, and relies on wages when the low state is less likely. We provide some observable implications of our contracting approach to ratings. (JEL G24, D86)
One Central Bank to Rule Them All*
Authors: Brusa Francesca , Savor Pavel , Wilson Mungo
Published: July 2019

While global stock markets enjoy high returns on days surrounding Federal Open Market Committee (FOMC) meetings, there is no comparable result for other central banks either internationally or, more surprisingly, domestically. Neither announcement surprises nor currency moves drive these findings, which hold even for stocks with a domestic focus. The difference in announcement premia is not explained by economy size, exposure to multinationals, or policy activism. We conclude that the Fed exerts a unique impact on global equities. Consistent with this hypothesis, uncertainty drops across global markets following FOMC announcements but not those of other central banks. Furthermore, the Fed is generally the leader among central banks in setting monetary policy.
A MULTICENTRE SURVEY OF LOCAL DIAGNOSTIC REFERENCE LEVELS AND ACHIEVABLE DOSE FOR CORONARY ANGIOGRAPHY AND PERCUTANEOUS TRANSLUMINAL CORONARY INTERVENTION PROCEDURES IN KOREA
Authors: Kim Jung Su , Lee Bong-Ki , Ryu Dong Ryeol , Chun Kwang Jin , Choi Hyun-Hee , Roh Younghoon , Kwon Soon-mu , Cho Byung-Ryul
Published: July 2019

Interventional cardiology procedures can involve relatively high radiation doses compared to general radiography. During coronary angiography (CAG) and percutaneous transluminal coronary intervention (PCI), the same area is exposed to radiation for a long period. In this study, radiation exposure data of 1071 examinations in Korean hospitals were collected, and the achievable dose (AD) and diagnostic reference levels (DRLs) in actual medical practice for two types of interventional cardiology procedures in Korea were established. In CAG, 75th percentile DRLs and AD of the total kerma-area product were 47.0 and 33.1 Gy·cm 2, respectively. In PCI, those values were 171.3 and 102.6 Gy·cm2, respectively. This is the first study to introduce the DRLs for cardiovascular interventional procedures in Korea. These results will help optimise the interventional cardiology procedures for Korean cardiac centres.
Energy efficient synaptic plasticity
Authors: Li Ho Ling , van Rossum Mark C. W.
Published: July 2019

Many aspects of the brain’s design can be understood as the result of evolutionary drive towards efficient use of metabolic energy. In addition to the energetic costs of neural computation and transmission, experimental evidence indicates that synaptic plasticity is metabolically demanding as well. As synaptic plasticity is crucial for learning, we examine how these metabolic costs enter in learning. We find that when synaptic plasticity rules are naively implemented, training neural networks requires extremely large amounts of energy when storing many patterns. We propose that this is avoided by precisely balancing labile forms of synaptic plasticity with more stable forms. This algorithm, termed synaptic caching, boosts energy efficiency manifold. Our results yield a novel interpretation of the multiple forms of neural synaptic plasticity observed experimentally, including synaptic tagging and capture phenomena. Furthermore our results are relevant for energy efficient neuromorphic designs.
Quantitative analysis ofin vivomethionine oxidation of the human proteome
Authors: Bettinger John Q. , Welle Kevin A. , Hryhorenko Jennifer R. , Ghaemmaghami Sina
Published: July 2019

The oxidation of methionine is an important posttranslational modification of proteins with numerous roles in physiology and pathology. However, the quantitative analysis of methionine oxidation on a proteome-wide scale has been hampered by technical limitations. Methionine is readily oxidizedin vitroduring sample preparation and analysis. In addition, there is a lack of enrichment protocols for peptides that contain an oxidized methionine residue; making the accurate quantification of methionine oxidation difficult to achieve on a global scale. Herein, we report a methodology to circumvent these issues by isotopically labeling unoxidized methionines with18O labeled hydrogen peroxide and quantifying the relative ratios of18O and16O oxidized methionines. We validate our methodology using artificially oxidized proteomes made to mimic varying degrees of methionine oxidation. Using this method, we identify and quantify a number of novel sites ofin vivomethionine oxidation in an unstressed human cell line.
Structural control for the coordinated assembly into functional pathogenic type-3 secretion systems
Authors: Goessweiner-Mohr Nikolaus , Kotov Vadim , Brunner Matthias J. , Mayr Julia , Wald Jiri , Kuhlen Lucas , Miletic Sean , Vesper Oliver , Lugmayr Wolfgang , Wagner Samuel , DiMaio Frank , Lea Susan , Marlovits Thomas C.
Published: July 2019

Functional injectisomes of the type-3 secretion system assemble into highly defined and stoichiometric bacterial molecular machines essential for infecting human and other eukaryotic cells. However, the mechanism that governs the regulated step-wise assembly process from the nucleation-phase, to ring-assembly, and the filamentous phase into a membrane embedded needle complex is unclear. We here report that the formation of a megadalton-sized needle complexes fromSalmonella entericaserovar Typhimurium (SPI-1,Salmonellapathogenicity island-1) with proper stoichiometries is highly structurally controlled competing against the self-assembly propensity of injectisome components, leading to a highly unusual structurally-pleiotropic phenotype. The structure of the entire needle complex from pathogenic injectisomes was solved by cryo electron microscopy, focused refinements (2.5-4 Å) and co-variation analysis revealing an overall asymmetric arrangement containing cyclic, helical, and asymmetric sub-structures. The centrally located export apparatus assembles into a conical, pseudo-helical structure and provides a structural template that guides the formation of a 24-mer cyclic, surrounding ring, which then serves as a docking interface comprising three different conformations for sixteen N-terminal InvG subunits of the outer secretin ring. Unexpectedly, the secretin ring excludes the 16thprotein chain at the C-terminal outer ring, resulting in a pleiotropic 16/15-mer ring and consequently to an overall 24:16/15 basal body structure. Finally, we report how the transition from the pseudo-helical export apparatus into the helical filament is structurally resolved to generate the protein secretion channel, which provides the structural basis to restrict access of unfolded effector substrates. These results highlight the diverse molecular signatures required for a highly coordinated assembly process and provide the molecular basis for understanding triggering and transport of unfolded proteins through injectisomes.
O-linked mucin-type glycosylation regulates the transcriptional programme downstream of EGFR in breast cancer
Authors: Tajadura-Ortega Virginia , Gambardella Gennaro , Skinner Alexandra , Schjoldager Katrine Ter-Borch Gram , Beatson Richard , Graham Rosalind , Achkova Daniela , Taylor-Papadimitriou Joyce , Ciccarelli Francesca D. , Burchell Joy M.
Published: July 2019

Aberrant mucin type O-linked glycosylation is a common occurrence in cancer. This type of O-linked glycosylation is not limited to mucins but can occur on many cell surface glycoproteins where only a small number of sites may be present. Upon EGF ligation, EGFR induces a signaling cascade but can also translocate to the nucleus where it can directly regulate gene transcription. Here we show that upon EGF binding, human breast cancer cells carrying different O-linked glycans respond by transcribing different gene expression signatures. This is not a result of changes in signal transduction but due to the differential nuclear translocation of EGFR in the two glyco-phenotypes. This is regulated by the formation of an EGFR/galectin-3/MUC1/β-catenin complex at the cell surface that is present in cells carrying short core-1-based O-glycans characteristic of tumour cells but absent in core-2-carrying cells.
Corvus: a framework for interfacing scientific software for spectroscopic and materials science applications
Authors: Story S. M. , Vila F. D. , Kas J. J. , Raniga K. B. , Pemmaraju C. D. , Rehr J. J.
Published: July 2019

Corvus, a Python-based package designed for managing workflows of physical simulations that utilize multiple scientific software packages, is presented. Corvus can be run as an executable script with an input file and automatically generated or custom workflows, or interactively, in order to build custom workflows with a set of Corvus-specific tools. Several prototypical examples are presented that link density functional, vibrational and X-ray spectroscopy software packages and are of interest to the synchrotron community. These examples highlight the simplification of complex spectroscopy calculations that were previously limited to expert users, and demonstrate the flexibility of the Corvus infrastructure to tackle more general problems in other research areas.
An asymptotic analysis for detailed mathematical modeling of counter-flow non-premixed multi-zone laminar flames fueled by lycopodium particles
Authors: Bidabadi Mehdi , Sadeghi Sadegh , Panahifar Pedram , Toghraie Davood , Rahbari Alireza
Published: July 2019

Purpose

This study aims to present a basic mathematical model for investigating the structure of counter-flow non-premixed laminar flames propagating through uniformly-distributed organic fuel particles considering preheat, drying, vaporization, reaction and oxidizer zones.

Design/methodology/approach

Lycopodium particles and air are taken as biofuel and oxidizer, respectively. Dimensionalized and non-dimensionalized forms of mass and energy conservation equations are derived for each zone taking into account proper boundary and jump conditions. Subsequently, to solve the governing equations, an asymptotic method is used. For validation purpose, results achieved from the present analysis are compared with reliable data reported in the literature under certain conditions.

Findings

With regard to the comparisons, although different complex non-homogeneous differential equations are solved in this paper, acceptable agreements are observed. Finally, the impacts of significant parameters including fuel and oxidizer Lewis numbers, equivalence ratio, mass particle concentration, fuel and oxidizer mass fractions and lycopodium initial temperature on the flame temperature, flame front position and flow strain rate are elaborately explained.

Originality/value

An asymptotic method for mathematical modeling of counter-flow non-premixed multi-zone laminar flames propagating through lycopodium particles.