bluuebunny/crossref_metadata_2025 huggingface dataset:
Establishment of Optogenetic Modulation of cAMP for Analyzing Growth, Biofilm Formation, and Virulence Pathways of Bacteria Using a Light-Gated Cyclase
Authors: Kaushik Manish Singh , Pati Swaroop Ranjan , Soni Shivanika , Mishra Ayushi , Sushmita Kumari , Kateriya Suneel
Published: August 2020

In bacteria, cyclic adenosine monophosphate (cAMP) signaling plays an essential regulatory role whose modulation via optogenetic tools would provide researchers an immense opportunity to control biological processes simply by illumination. The cAMP signaling in bacteria is a complex network of regulatory pathways, which utilizes distinct proteomic resources under different nutrient environments. We established an optogenetic modulation of cAMP and studied important cellular process of growth, biofilm formation, and virulence in the model bacterium E. coli using a light-gated adenylate cyclase (LgAC) from Beggiatoa sp. Blue light-induced activation of LgAC elevated the cAMP level in a blue light-dependent manner in E. coli. Quantitative proteomics revealed a decrease in the level of certain proteins governing growth (PTS, Adk, AckA, GlnA, and EFP), biofilm formation (IhfA, flagellin, YajQ, YeaG, and HlfC), and virulence (ClpP, YebC, KatE, BtuE, and Zur) in E. coli cells expressing LgAC upon blue light illumination. This optogenetic modulation of cAMP would be useful for deciphering cAMP-associated host–pathogen signaling of bacterial systems. Proteome knowledge established by this research work would also be useful for the scientific community while adapting LgAC-based optogenetic modulation for studying other relevant cAMP-driven bacterial physiology (e.g., energy metabolism). The systematic utilization of the established method and more extensively designed experiments regarding bacterial growth, biofilm, survival, and virulence might provide a road map for the identification of new targets for developing novel antibacterial drugs.
Electrochemical Ion Pumping Device for Blue Energy Recovery: Mixing Entropy Battery
Authors: Galleguillos Felipe , Cáceres Luis , Maxwell Lindley , Soliz Álvaro
Published: August 2020

In the process of finding new forms of energy extraction or recovery, the use of various natural systems as potential clean and renewable energy sources has been examined. Blue energy is an interesting energy alternative based on chemical energy that is spontaneously released when mixing water solutions with different salt concentrations. This occurs naturally in the discharge of rivers into ocean basins on such a scale that it justifies efforts for detailed research. This article collects the most relevant information from the latest publications on the topic, focusing on the use of the mixing entropy battery (MEB) as an electrochemical ion pumping device and the different technological means that have been developed for the conditions of this process. In addition, it describes various practices and advances achieved by various researchers in the optimization of this device, in relation to the most important redox reactions and the cathode and anodic materials used for the recovery of blue energy or salinity gradient energy.
Numerical Investigation of Methodologies for Cavitation Suppression Inside Globe Valves
Authors: Li Jun-ye , Gao Zhi-xin , Wu Hui , Jin Zhi-jiang
Published: August 2020

Cavitation inside globe valves, which is a common phenomenon if there is a high-pressure drop, is numerically investigated in this study. Firstly, the cavitation phenomenon in globe valves with a different number of cages is compared. When there is no valve cage, cavitation mainly appears at the valve seat, the bottom of the valve core, and the downstream pipelines. By installing a valve cage, cavitation bubbles can be restricted around the valve cage protecting the valve body from being damaged. Secondly, the effects of the outlet pressure, the working temperature, and the installation angle of two valve cages in a two-cage globe valve are studied to find out the best method to suppress cavitation, and cavitation number is utilized to evaluate cavitation intensity. Results show that cavitation intensity inside globe valves can be reduced by increasing the valve outlet pressure, decreasing the working temperature, or increasing the installation angle. Results suggest that increasing the outlet pressure is the most efficient way to suppress cavitation intensity in a globe valve, and the working temperature has a minimal effect on cavitation intensity.
Rolling Bearings Fault Diagnosis Based on Improved Complete Ensemble Empirical Mode Decomposition with Adaptive Noise, Nonlinear Entropy, and Ensemble SVM
Authors: Li Rui , Ran Chao , Zhang Bin , Han Leng , Feng Song
Published: August 2020

Rolling bearings are fundamental elements that play a crucial role in the functioning of rotating machines; thus, fault diagnosis of rolling bearings is of great significance to reduce catastrophic failures and heavy economic loss. However, the vibration signals of rolling bearings are often nonlinear and nonstationary, resulting in difficulty for feature extraction and fault recognition. In this paper, a hybrid method for multiple fault diagnosis of rolling bearings is presented. The bearing vibration signals are decomposed with the improved complete ensemble empirical mode decomposition with adaptive noise (ICEEMDAN) to denoise and extract nonlinear entropy features. The nonlinear entropy features are further processed to select the more discriminative fault features and to reduce feature dimension. Then a multi-class intelligent recognition model based on ensemble support vector machine (ESVM) is constructed to diagnose different bearing fault modes as well as fault severities. The effectiveness of the proposed method is assessed via experimental case studies of rolling bearings under multiple operational conditions (i.e., speeds and loads). The results show that our method gives better diagnosis results as compared to some existing approaches.
Sampling Rate of Polar Organic Chemical Integrative Sampler (POCIS): Influence Factors and Calibration Methods
Authors: Wang Liyang , Liu Ruixia , Liu Xiaoling , Gao Hongjie
Published: August 2020

As a passive sampling device, the polar organic chemical integrative sampler (POCIS) has the characteristics of simple operation, safety, and reliability for assessing the occurrence and risk of persistent and emerging trace organic pollutants. The POCIS, allowing for the determination of time-weighted average (TWA) concentration of polar organic chemicals, exhibits good application prospects in aquatic environments. Before deploying the device in water, the sampling rate (Rs), which is a key parameter for characterizing pollutant enrichment, should be determined and calibrated accurately. However, the Rs values strongly depend on experimental hydrodynamic conditions. This paper provides an overview of the current situation of the POCIS for environmental monitoring of organic pollutants in an aquatic system. The principle and theory of the POCIS are outlined. In particular, the effect factors such as the ambient conditions, pollutant properties, and device features on the Rs are analyzed in detail from aspects of impact dependence and mechanisms. The calibration methods of the Rs under laboratory and in situ conditions are summarized. This review offers supplementary information on comprehensive understanding of mechanism and application of the POCIS. Nevertheless, the Rs were impacted by a combined effect of solute–sorbent–membrane–solution, and the influence extent of each variable was still unclear. On this basis, the ongoing challenges are proposed for the future application of the POCIS in the actual environment, for instance, the need for this device to be improved in terms of quantitative methods for more accurate measurement of the Rs.
Tunable Plasmon-Induced Transparency through Bright Mode Resonator in a Metal–Graphene Terahertz Metamaterial
Authors: Wang Guanqi , Zhang Xianbin , Wei Xuyan
Published: August 2020

The combination of graphene and metamaterials is the ideal route to achieve active control of the electromagnetic wave in the terahertz (THz) regime. Here, the tunable plasmon-induced transparency (PIT) metamaterial, integrating metal resonators with tunable graphene, is numerically investigated at THz frequencies. By varying the Fermi energy of graphene, the reconfigurable coupling condition is actively modulated and continuous manipulation of the metamaterial resonance intensity is achieved. In this device structure, monolayer graphene operates as a tunable conductive film which yields actively controlled PIT behavior and the accompanied group delay. This device concept provides theoretical guidance to design compact terahertz modulation devices.
Influences of Global and Local Features on Eye-Movement Patterns in Visual-Similarity Perception of Synthesized Texture Images
Authors: Guo Xiaoying , Li Liang , Asano Akira , Muraki Asano Chie
Published: August 2020

Global and local features are essential for visual-similarity texture perception. Therefore, understanding how people allocate their visual attention when viewing textures with global or local similarity is important. In this work, we investigate the influences of global and local features of a texture on eye-movement patterns and analyze the relationship between eye-movement patterns and visual-similarity selection. First, we synthesized textures by separately controlling global and local textural features through the primitive, grain, and point configuration (PGPC) texture model, a mathematical morphology-based texture model. Second, we conducted an experiment to acquire eye-movement data where participants identified the texture that was highly similar to the standard texture. Experiment data were obtained through an eye-tracker from 60 participants. The collected eye-tracking data were analyzed in terms of three metrics, including total fixation duration in each region of interest (ROI), fixation-point variance in each ROI, and fixation-transfer counts between different ROIs. Analysis results indicated the following. (1) The global and local features of a texture influenced eye-movement patterns. In particular, the texture image that was globally similar to the standard texture contained dispersed fixation points. By contrast, the texture image that was locally similar to the standard texture contained concentrated fixation points. The domination of global and local features influenced the viewers’ similarity choice. (2) The final visual-similarity selection was related to the fixation-transfer count between different ROIs, but not to the fixation time in each ROI. This research also extends the applicability of the mathematical morphology-based texture model to human visual perception.
Contrasting Electron and Hole Transfer Dynamics from CH(NH2)2PbI3 Perovskite Quantum Dots to Charge Transport Layers
Authors: Lou Zhigang , Liang Shuyan , Yuan Jiabei , Ji Kang , Yuan Jianyu , Zhao Hongchang , Xia Hong , Ni Gang , Sheng Chuanxiang , Ma Wanli , Chen Liangyao , Zhao Haibin
Published: August 2020

In this work, the ultrafast transient absorption spectroscopy (TAs) was utilized to first investigate the charge transfer from the emerging FAPbI3 (FA = CH(NH2)2) perovskite quantum dots (PQDs) to charge transport layers. Specifically, we compared the TAs in pure FAPbI3 PQDs, PQDs grown with both electron and hole transfer layers (ETL and HTL), and PQDs with only ETL or HTL. The TA signals induced by photoexcited electrons decay much faster in PQDs samples with the ETL (~20 ps) compared to the pure FAPbI3 PQDs (>1 ns). These results reveal that electrons can effectively transport between coupled PQDs and transfer to the ETL (TiO2) at a time scale of 20 ps, much faster than the bimolecular charge recombination inside the PQDs (>1 ns), and the electron transfer efficiency is estimated to be close to 100%. In contrast, the temporal evolution of the TA signals in the PQDs with and without HTL exhibit negligible change, and no substantive hole transfer to the HTL (poly[bis(4-phenyl)(2,4,6-trimethylphenyl)amine], PTAA) occurs within 1 ns. The much slower hole transfer implies the further potential of increasing the overall photo-carrier conversion efficiency through enhancing the hole diffusion length and fine-tuning the coupling between the HTL and PQDs.
Interplay between Cell-Surface Receptors and Extracellular Matrix in Skin
Authors: Kleiser Svenja , Nyström Alexander
Published: August 2020

Skin consists of the epidermis and dermis, which are connected by a specialized basement membrane—the epidermal basement membrane. Both the epidermal basement membrane and the underlying interstitial extracellular matrix (ECM) created by dermal fibroblasts contain distinct network-forming macromolecules. These matrices play various roles in order to maintain skin homeostasis and integrity. Within this complex interplay of cells and matrices, cell surface receptors play essential roles not only for inside-out and outside-in signaling, but also for establishing mechanical and biochemical properties of skin. Already minor modulations of this multifactorial cross-talk can lead to severe and systemic diseases. In this review, major epidermal and dermal cell surface receptors will be addressed with respect to their interactions with matrix components as well as their roles in fibrotic, inflammatory or tumorigenic skin diseases.
Bioelectric Sensors: On the Road for the 4.0 Diagnostics and Biomedtech Revolution
Authors: Kintzios Spyridon
Published: August 2020

Bioelectric sensors lie, by definition, on the interface between biological elements and electronic circuits, irrespective of scale, manufacturing method, and working principle [...]
Percutaneous Discectomy Followed by CESI Might Improve Neurological Disorder of Drop Foot Patients Due to Chronic LDH
Authors: Budisulistyo Trianggoro , Atmaja Firmansyah
Published: August 2020

(1) Introduction: Epiconus and conus medullary syndromes that consisted of drop foot, pain, numbness, bladder or bowel dysfunction are serious problems might be caused by lumbar disc(s) herniation (LDH) compression. (2) Objective: To evaluate percutaneous discectomy effectivity for decompressing LDH lesions. (3) Case Report: Three patients suffered from drop feet, numbness, and bowel and bladder problems due to LDH compression. Patient #1 is a male (35 years old, basal metabolism index (BMI) = 23.9), point 1 on manual muscle test (MMT), with protrusion on L3 to S1 discs; Patient #2 is a female (62 years old, BMI = 22.4), point 3 on MMT, with protrusion on L2-4 and L5-S1 discs; Patient #3 is a female (43 years old, BMI = 26.6), point 4 on MMT, with extrusion on T12-L1 and L1-2 and L3-4 protruded discs. Six months follow-up showed of stand and walkability improvement with Patient #1 and #2. Patient #3 showed improvement in bowel and bladder problems within 10 weeks, without suffering of postoperative pain syndromes. (4) Discussion: Patient #1 and #2 showed better outcomes than Patient #3 who affected epiconus and cauda equina syndromes. Triamcinolone and lidocaine have analgesic and anti-inflammatory properties for improving intraepidural circulation adjacent to the lesion sites. (5) Conclusion: Drop foot caused by mechanical compression of LDH ought to be treated immediately. Lateral or posterolateral compression has better outcomes associated with anatomical structures. Discectomy through transforaminal approach that is followed by caudal epidural steroid injection (CESI) under fluoroscopic guidance is a safer and minimally invasive treatment with promising outcomes.
Altered Organelle Calcium Transport in Ovarian Physiology and Cancer
Authors: Caravia Laura , Staicu Cristina Elena , Radu Beatrice Mihaela , Condrat Carmen Elena , Crețoiu Dragoș , Bacalbașa Nicolae , Suciu Nicolae , Crețoiu Sanda Maria , Voinea Silviu Cristian
Published: August 2020

Calcium levels have a huge impact on the physiology of the female reproductive system, in particular, of the ovaries. Cytosolic calcium levels are influenced by regulatory proteins (i.e., ion channels and pumps) localized in the plasmalemma and/or in the endomembranes of membrane-bound organelles. Imbalances between plasma membrane and organelle-based mechanisms for calcium regulation in different ovarian cell subtypes are contributing to ovarian pathologies, including ovarian cancer. In this review, we focused our attention on altered calcium transport and its role as a contributor to tumor progression in ovarian cancer. The most important proteins described as contributing to ovarian cancer progression are inositol trisphosphate receptors, ryanodine receptors, transient receptor potential channels, calcium ATPases, hormone receptors, G-protein-coupled receptors, and/or mitochondrial calcium uniporters. The involvement of mitochondrial and/or endoplasmic reticulum calcium imbalance in the development of resistance to chemotherapeutic drugs in ovarian cancer is also discussed, since Ca2+ channels and/or pumps are nowadays regarded as potential therapeutic targets and are even correlated with prognosis.
PCR-Free Detection of Long Non-Coding HOTAIR RNA in Ovarian Cancer Cell Lines and Plasma Samples
Authors: Soda Narshone , Umer Muhammad , Kashaninejad Navid , Kasetsirikul Surasak , Kline Richard , Salomon Carlos , Nguyen Nam-Trung , Shiddiky Muhammad J. A.
Published: August 2020

Long non-coding RNA HOX transcript antisense intergenic RNA (HOTAIR) is one of the promising biomarkers that has widely been used in determining the stages of many cancers, including ovarian cancer. In cancer diagnostics, the two key analytical challenges for detecting long non-coding RNA biomarkers are i) the low concentration levels (nM to fM range) in which they are found and ii) the analytical method where broad dynamic range is required (four to six orders of magnitude) due to the large variation in expression levels for different HOTAIR RNAs. To meet these challenges, we report on a biosensing platform for the visual (colorimetric) estimation and subsequent electrochemical quantification of ovarian-cancer-specific HOTAIR using a screen-printed gold electrode (SPE-Au). Our assay utilizes a two-step strategy that involves (i) magnetic isolation and purification of target HOTAIR sequences and (ii) subsequent detection of isolated sequences using a sandwich hybridization coupled with horseradish peroxidase (HRP)-catalyzed reaction of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. The assay achieved a detection limit of 1.0 fM HOTAIR in spiked buffer samples with excellent reproducibility (% RSD ≤ 5%, for n = 3). It was successfully applied to detect HOTAIR in cancer cell lines and a panel of plasma samples derived from patients with ovarian cancer. The analytical performance of the method was validated with standard RT-qPCR. We believe that the proof of concept assay reported here may find potential use in routine clinical settings for the screening of cancer-related lncRNAs.
Predicting Peritoneal Dissemination of Gastric Cancer in the Era of Precision Medicine: Molecular Characterization and Biomarkers
Authors: Chen Yanyan , Zhou Quan , Wang Haiyong , Zhuo Wei , Ding Yongfeng , Lu Jun , Wu Guanghao , Xu Nong , Teng Lisong
Published: August 2020

Gastric cancer (GC) is a leading cause of worldwide cancer-related death. Being a highly heterogeneous disease, the current treatment of GC has been suboptimal due to the lack of subtype-dependent therapies. Peritoneal dissemination (PD) is a common pattern of GC metastasis associated with poor prognosis. Therefore, it is urgently necessary to identify patients at high risk of PD. PD is found to be associated with Lauren diffuse type GC. Molecular profiling of GC, especially diffuse type GC, has been utilized to identify molecular alterations and has given rise to various molecular classifications, shedding light on the underlying mechanism of PD and enabling identification of patients at higher PD risk. In addition, a series of diagnositc and prognostic biomarkers of PD from serum, peritoneal lavages and primary GCs have been reported. This comprehensive review summarizes findings on the multi-omic characteristics of diffuse type GC, the clinical significance of updating molecular classifications of GC in association with PD risk and research advances in PD-associated biomarkers.
The Multifunctionality of CD36 in Diabetes Mellitus and Its Complications—Update in Pathogenesis, Treatment and Monitoring
Authors: Puchałowicz Kamila , Rać Monika Ewa
Published: August 2020

CD36 is a multiligand receptor contributing to glucose and lipid metabolism, immune response, inflammation, thrombosis, and fibrosis. A wide range of tissue expression includes cells sensitive to metabolic abnormalities associated with metabolic syndrome and diabetes mellitus (DM), such as monocytes and macrophages, epithelial cells, adipocytes, hepatocytes, skeletal and cardiac myocytes, pancreatic β-cells, kidney glomeruli and tubules cells, pericytes and pigment epithelium cells of the retina, and Schwann cells. These features make CD36 an important component of the pathogenesis of DM and its complications, but also a promising target in the treatment of these disorders. The detrimental effects of CD36 signaling are mediated by the uptake of fatty acids and modified lipoproteins, deposition of lipids and their lipotoxicity, alterations in insulin response and the utilization of energy substrates, oxidative stress, inflammation, apoptosis, and fibrosis leading to the progressive, often irreversible organ dysfunction. This review summarizes the extensive knowledge of the contribution of CD36 to DM and its complications, including nephropathy, retinopathy, peripheral neuropathy, and cardiomyopathy.
Cancer Stem Cells, Quo Vadis? The Notch Signaling Pathway in Tumor Initiation and Progression
Authors: Meisel Christian T. , Porcheri Cristina , Mitsiadis Thimios A.
Published: August 2020

The Notch signaling pathway regulates cell proliferation, cytodifferentiation and cell fate decisions in both embryonic and adult life. Several aspects of stem cell maintenance are dependent from the functionality and fine tuning of the Notch pathway. In cancer, Notch is specifically involved in preserving self-renewal and amplification of cancer stem cells, supporting the formation, spread and recurrence of the tumor. As the function of Notch signaling is context dependent, we here provide an overview of its activity in a variety of tumors, focusing mostly on its role in the maintenance of the undifferentiated subset of cancer cells. Finally, we analyze the potential of molecules of the Notch pathway as diagnostic and therapeutic tools against the various cancers.
CO2 Capture in A Bubble-Column Scrubber Using MEA/CaCl2/H2O Solution—Absorption and Precipitation
Authors: Chen Pao Chi , Zhuo Shiun Huang
Published: August 2020

This study used the solvent monoethylamine (MEA)/CaCl2/H2O to investigate CO2 absorption and CaCO3 crystallization in a bubble column scrubber. The variables explored were pH, gas flow rate, gas concentration, the liquid flow rate of the solution to absorb CO2, and CaCO3 crystallization. Under a continuous mode, the solution of CaCl2 was fed continuously, and the pH dropped after CO2 absorption. To maintain the set pH value, there was an automatic input of the MEA solvent into the bubble column. In addition to maintaining the pH, the solution could also absorb CO2 and produce CaCO3 crystals, which served two purposes. The results showed that there were mainly vaterite crystals. At different pH values, the lower the pH, the higher the precipitation rate of vaterite (Fp), and vice versa. However, under different gas flow rates, the Fp decreased as the pH value increased. Additionally, the process variables also affected the absorption rate (RA) and the overall mass-transfer coefficient (KGa) generally increased with increasing pH, gas concentration, and gas flow rate. However, it slowed down under operating conditions at high pH and high gas flow rate. Finally, correlation equations for RA, KGa, and Fp were also obtained and discussed in the study.
Ethical Data Collection and Recognizing the Impact of Semi-Structured Interviews on Research Respondents
Authors: Husband Gary
Published: August 2020

This article explores the complex relationship between researcher and respondent through shared experience and interaction in the interview processes. Ethical considerations related to the balance of power and potential for change in respondents’ professional actions and decisions post-interview are discussed whilst problematizing the concept of truly informed consent. Informed by critical incident analysis, the article draws on the researcher’s experience of undertaking a qualitative-based study founded in the principles of phenomenological hermeneutics. Concluded in 2016, the research investigates the impact of pedagogical training programs on respondents’ teaching practice and engagement with professional learning. Respondents, experienced lecturers working in the adult education sectors in Scotland and Wales, contacted the researcher to share their post-interview experiences. The research was not designed to elicit change in respondents, nor influence professional choice or practice. However, each communication received attributed participating in the research as the source for renewed interest and engagement in professional learning. Although research interviews becoming an enriching experience for respondents is a recorded phenomenon the ascribed effects were profound, potentially life-changing, and not fully anticipated. Ethical considerations for researchers designing and undertaking interview-based research are considered alongside the potential for engagement in research interviews as a catalyst for professional learning in practice.
Site Classification of Eucalyptus urophylla × Eucalyptus grandis Plantations in China
Authors: Lu Haifei , Xu Jianmin , Li Guangyou , Liu Wangshu
Published: August 2020

Background and Objectives: It is important to match species needs with site conditions for sustainable forestry. In Eucalyptus urophylla × Eucalyptus grandis plantations in southern Yunnan, China, species-site mismatches have led to inappropriate expansion and management, which has degraded forests and decreased efficiency in plantation production. Further research is needed to understand the relationship between tree growth and site productivity. We empirically explored site features and classified site types within these plantations in southern Yunnan. Our objective was to develop a theoretical basis for improving site selection for afforestation, and to establish intensive management in that region. Materials and Methods: 130 standard plots were set up in 1−15-year-old eucalyptus plantations in Pu’er and Lincang. We used quantification theory to examine the relationship between dominant tree growth traits and site factors. Hierarchical cluster analysis and canonical correlation analysis were applied to classify sites and evaluate the growth potential of E. urophylla × E. grandis plantations, respectively. Results: The multiple correlation coefficient between eight site factors (altitude, slope, slope position, aspect, soil depth, texture, bulk density, and litter thickness) and the quantitative growth of the dominant tree was 0.834 (p < 0.05). Slope position, altitude, and soil depth were the main factors contributing to the variation in stand growth. Plantation growth was best on lower slopes at relatively low altitude, where thick and weathered red soil layers existed. Conversely, the poorest plantations were located on upper slopes at higher altitude, with a thin semi-weathered purple soil layer. The soil factors total nitrogen (N) and potassium (K), trace boron (B), copper (Cu), and zinc (Zn) content, available phosphorous (P), and organic matter content in the soil influenced plantation growth. Conclusions. The addition of N, P, and K fertilizer as well as trace elements such as B, Cu, and Zn can promote the productivity of these plantations.
Dry Country, Wet City: A World-Ecological Reading of Drought in Thea Astley’s Drylands
Authors: Cahillane Ashley
Published: August 2020

Using a postcolonial and world-ecological framework, this article analyses the representation of water as an energy source in Thea Astley’s last and most critically acclaimed novel Drylands (1999). As environmental historians have argued, the colonial, and later capitalist, settlement of Australia, particularly the arid interior, was dependent on securing freshwater sources—a historical process that showed little regard for ecological impact or water justice until recent times. Drylands’ engagement with this history will be considered in relation to Michael Cathcart’s concept of ‘water dreaming’ (2010): the way in which water became reimagined after colonization to signify the prospect of economic growth and the consolidation of settler belonging. Drylands self-consciously incorporates predominant modes of ‘water dreaming’ into its narrative, yet resists reducing water to a passive resource. This happens on the level of both content and form: while its theme of drought-induced migration is critical of the past, present, and future social and ecological effects of the reckless extraction of freshwater, its nonlinear plot and hybrid form as a montage of short stories work to undermine the dominant anthropocentric colonial narratives that underline technocratic water cultivation.
Antibiotic Resistance of Legionella pneumophila in Clinical and Water Isolates—A Systematic Review
Authors: Pappa Olga , Chochlakis Dimosthenis , Sandalakis Vassilios , Dioli Chrysa , Psaroulaki Anna , Mavridou Athena
Published: August 2020

The current systematic review investigates the antibiotic susceptibility pattern of Legionella pneumophila isolates from the 1980s to the present day, deriving data from clinical and/or water samples from studies carried out all over the world. Eighty-nine papers meeting the inclusion criteria, i.e., “Legionella pneumophila” and “resistance to antibiotics”, were evaluated according to pre-defined validity criteria. Sixty articles referred to clinical isolates, and 18 articles reported water-related L. pneumophila isolates, while 11 articles included both clinical and water isolates. Several methods have been proposed as suitable for the determination of MICs, such as the E-test, broth and agar dilution, and disk diffusion methods, in vivo and in vitro, using various media. The E-test method proposed by the European Society of Clinical Microbiology and Infectious Diseases (EUCAST) seems to be the second most frequently used method overall, but it is the preferred method in the most recent publications (2000–2019) for the interpretation criteria. Erythromycin has been proved to be the preference for resistance testing over the years. However, in the last 19 years, the antibiotics ciprofloxacin (CIP), erythromycin (ERM), levofloxacin (LEV) and azithromycin (AZM) were the ones that saw an increase in their use. A decrease in the sensitivity to antibiotics was identified in approximately half of the reviewed articles.
Index for the Consistent Measurement of Spatial Heterogeneity for Large-Scale Land Cover Datasets
Authors: Yu Jing , Peng Shu , Zhang Weiwei , Kang Shun
Published: August 2020

Recognizing land cover heterogeneity is essential for the assessment of spatial patterns to guide conservation planning. One of the top research priorities is the quantification of land cover heterogeneity using effective landscape metrics. However, due to the diversity of land cover types and their varied distribution, a consistent, larger-scale, and standardized framework for heterogeneity information extraction from this complex perspective is still lacking. Consequently, we developed a new Land Cover Complexity Index (LCCI), which is based on information-theory. The LCCI contains two foundational aspects of heterogeneity, composition and configuration, thereby capturing more comprehensive information on land cover patterns than any single metric approach. In this study, we compare the performance of the LCCI with that of other landscape metrics at two different scales, and the results show that our newly developed indicator more accurately characterizes and distinguishes different land cover patterns. LCCI provides an alternative way to measure the spatial variation of land cover distribution. Classification maps of land cover heterogeneity generated using the LCCI provide valuable insights and implications for regional conservation planning. Thus, the LCCI is shown to be a consistent indicator for the quantification of land cover heterogeneity that functions in an adaptive way by simultaneously considering both composition and configuration.
Indomethacin Increases Quercetin Affinity for Human Serum Albumin: A Combined Experimental and Computational Study and Its Broader Implications
Authors: Rimac Hrvoje , Tandarić Tana , Vianello Robert , Bojić Mirza
Published: August 2020

Human serum albumin (HSA) is the most abundant carrier protein in the human body. Competition for the same binding site between different ligands can lead to an increased active concentration or a faster elimination of one or both ligands. Indomethacin and quercetin both bind to the binding site located in the IIA subdomain. To determine the nature of the HSA-indomethacin-quercetin interactions, spectrofluorometric, docking, molecular dynamics studies, and quantum chemical calculations were performed. The results show that the indomethacin and quercetin binding sites do not overlap. Moreover, the presence of quercetin does not influence the binding constant and position of indomethacin in the pocket. However, binding of quercetin is much more favorable in the presence of indomethacin, with its position and interactions with HSA significantly changed. These results provide a new insight into drug-drug interactions, which can be important in situations when displacement from HSA or other proteins is undesirable or even desirable. This principle could also be used to deliberately prolong or shorten the xenobiotics’ half-life in the body, depending on the desired outcomes.
Aptamers against Immunoglobulins: Design, Selection and Bioanalytical Applications
Authors: Bognár Zsófia , Gyurcsányi Róbert E.
Published: August 2020

Nucleic acid aptamers show clear promise as diagnostic reagents, as highly specific strands were reported against a large variety of biomarkers. They have appealing benefits in terms of reproducible generation by chemical synthesis, controlled modification with labels and functionalities providing versatile means for detection and oriented immobilization, as along with high biochemical and temperature resistance. Aptamers against immunoglobulin targets—IgA, IgM, IgG and IgE—have a clear niche for diagnostic applications, therefore numerous aptamers have been selected and used in combination with a variety of detection techniques. The aim of this review is to overview and evaluate aptamers selected for the recognition of antibodies, in terms of their design, analytical properties and diagnostic applications. Aptamer candidates showed convincing performance among others to identify stress and upper respiratory tract infection through SIgA detection, for cancer cell recognition using membrane bound IgM, to detect and treat hemolytic transfusion reactions, autoimmune diseases with IgG and detection of IgE for allergy diseases. However, in general, their use still lags significantly behind what their claimed benefits and the plethora of application opportunities would forecast.
Synthesis, Anticonvulsant and Antinociceptive Activity of New Hybrid Compounds: Derivatives of 3-(3-Methylthiophen-2-yl)-pyrrolidine-2,5-dione
Authors: Góra Małgorzata , Czopek Anna , Rapacz Anna , Dziubina Anna , Głuch-Lutwin Monika , Mordyl Barbara , Obniska Jolanta
Published: August 2020

The present study aimed to design and synthesize a new series of hybrid compounds with pyrrolidine-2,5-dione and thiophene rings in the structure as potential anticonvulsant and antinociceptive agents. For this purpose, we obtained a series of new compounds and evaluated their anticonvulsant activity in animal models of epilepsy (maximal electroshock (MES), psychomotor (6 Hz), and subcutaneous pentylenetetrazole (scPTZ) seizure tests). To determine the mechanism of action of the most active anticonvulsant compounds (3, 4, 6, 9), their influence on the voltage-gated sodium and calcium channels as well as GABA transporter (GAT) was assessed. The most promising compound 3-(3-methylthiophen-2-yl)-1-(3-morpholinopropyl)pyrrolidine-2,5-dione hydrochloride (4) showed higher ED50 value than those of the reference drugs: valproic acid (VPA) and ethosuximide (ETX) (62.14 mg/kg vs. 252.7 mg/kg (VPA) in the MES test, and 75.59 mg/kg vs. 130.6 mg/kg (VPA) and 221.7 mg/kg (ETX) in the 6 Hz test, respectively). Moreover, in vitro studies of compound 4 showed moderate but balanced inhibition of the neuronal voltage-sensitive sodium (site 2) and L-type calcium channels. Additionally, the antinociceptive activity of the most active compounds (3, 4, 6, 9) was also evaluated in the hot plate test and writhing tests, and their hepatotoxic properties in HepG2 cells were also investigated. To determine the possible mechanism of the analgesic effect of compounds 3, 6, and 9, the affinity for the TRPV1 receptor was investigated.
Physico-Chemical and Light-Induced Properties of Quinoline Azo-dyes Polymers
Authors: Chomicki Dariusz , Kharchenko Oksana , Skowronski Lukasz , Kowalonek Jolanta , Kozanecka-Szmigiel Anna , Szmigiel Dariusz , Smokal Vitalii , Krupka Oksana , Derkowska-Zielinska Beata
Published: August 2020

We present investigation of optical and photochromic properties as well as of surface quality of thin films of novel methacrylic polymers with 8-hydroxyquinoline azo-dyes in side-chain. Additionally, thermal stability of polymer powders was examined and their glass transition temperature was determined. Optical properties (extinction coefficient and refractive index) were determined by spectroscopic ellipsometry (SE) combined with absorbance measurements. Photoresponsive behavior was investigated by determination of photoisomerization rates under irradiation with unpolarized 365 nm light, as well as by conduction of holographic grating inscription experiment. Thin film quality was determined by atomic force microscopy (AFM) measurements. Thermal analysis was performed by thermogravimetric (TG), derivative thermogravimetric (DTG) and differential scanning calorimetry (DSC) measurements. We found that optical properties as well as photoisomerization rates of investigated polymers are dependent on the substituent in the para position of the phenyl ring. Surface relief grating inscription was successfully generated only for materials with chromophores containing dimethylamino (N(CH3)2) and methyl (CH3) substituents, but all materials exhibited birefringence grating in the bulk. Surface of most thin films was very smooth, but its quality was impaired by neutral (H) as well as carboxyl (COOH) substituent. Thermal stability of copolymers with side-chain chromophores was improved compared to pure poly(methyl methacrylate) (PMMA).
Decreased Expression of ZNF554 in Gliomas is Associated with the Activation of Tumor Pathways and Shorter Patient Survival
Authors: Balogh Andrea , Reiniger Lilla , Hetey Szabolcs , Kiraly Peter , Toth Eszter , Karaszi Katalin , Juhasz Kata , Gelencser Zsolt , Zvara Agnes , Szilagyi Andras , Puskas Laszlo G. , Matko Janos , Papp Zoltan , Kovalszky Ilona , Juhasz Csaba , Than Nandor Gabor
Published: August 2020

Zinc finger protein 554 (ZNF554), a member of the Krüppel-associated box domain zinc finger protein subfamily, is predominantly expressed in the brain and placenta in humans. Recently, we unveiled that ZNF554 regulates trophoblast invasion during placentation and its decreased expression leads to the early pathogenesis of preeclampsia. Since ZNF proteins are immensely implicated in the development of several tumors including malignant tumors of the brain, here we explored the pathological role of ZNF554 in gliomas. We examined the expression of ZNF554 at mRNA and protein levels in normal brain and gliomas, and then we searched for genome-wide transcriptomic changes in U87 glioblastoma cells transiently overexpressing ZNF554. Immunohistochemistry of brain tissues in our cohort (n = 62) and analysis of large TCGA RNA-Seq data (n = 687) of control, oligodendroglioma, and astrocytoma tissues both revealed decreased expression of ZNF554 towards higher glioma grades. Furthermore, low ZNF554 expression was associated with shorter survival of grade III and IV astrocytoma patients. Overexpression of ZNF554 in U87 cells resulted in differential expression, mostly downregulation of 899 genes. The “PI3K-Akt signaling pathway”, known to be activated during glioma development, was the most impacted among 116 dysregulated pathways. Most affected pathways were cancer-related and/or immune-related. Congruently, cell proliferation was decreased and cell cycle was arrested in ZNF554-transfected glioma cells. These data collectively suggest that ZNF554 is a potential tumor suppressor and its decreased expression may lead to the loss of oncogene suppression, activation of tumor pathways, and shorter survival of patients with malignant glioma.
Correction: Wu, Q.-L.; et al. Estimation of the Potential Infestation Area of Newly-Invaded Fall Armyworm Spodoptera Frugiperda in the Yangtze River Valley of China. Insects 2019, 10, 298
Authors: Wu Qiu-Lin , He Li-Mei , Shen Xiu-Jing , Jiang Yu-Ying , Liu Jie , Hu Gao , Wu Kong-Ming
Published: August 2020

The authors wish to make the following correction to this paper [...]
Laparoscopic Outcomes after Normal Clinical and Ultrasound Findings in Young Women with Chronic Pelvic Pain: A Cross-Sectional Study
Authors: Tempest Nicola , Efstathiou Ekaterina , Petros Zena , Hapangama Dharani K.
Published: August 2020

Chronic pelvic pain (CPP) is one of the most common chronic pain problems experienced by women, with prevalence rates comparable to asthma and back pain. However, it is poorly understood and causative pathology is only seldom found. We aimed to establish prevalence of abnormal findings at diagnostic laparoscopy in young women with CPP after normal findings at clinical examination and pelvic ultrasound scan. Information was retrospectively collected on all laparoscopies undertaken on women aged 16–30 years with normal preoperative findings over a 24-month period. One-hundred-and-fifty women (mean age 25 years and BMI 24.5) were included with laparoscopic examination revealing normal anatomy in 110 (73.3%) and pathology in 40 (27.2%). Endometriosis was detected in 30 (20%); 25 (16.7%) stage 1, 2 (1.3%) stage 2, 2 (1.3%) stage 3 and 1 (0.7%) stage 4. Most laparoscopies carried out on young women with CPP and normal clinical examination and pelvic ultrasound scan showed no significant clinical stigmata of pelvic disease. Women should be fully informed of the multifactorial nature of CPP and there should be a comprehensive management pathway for these women, as proceeding with invasive laparoscopy does not provide additional benefit when investigating CPP in the context of risk, cost and effect on long-term wellbeing.
Reliability and Validity of the Dyskinesia Impairment Scale in Children and Young Adults with Inherited or Idiopathic Dystonia
Authors: Danielsson Annika , Vanmechelen Inti , Lidbeck Cecilia , Krumlinde-Sundholm Lena , Ortibus Els , Monbaliu Elegast , Tedroff Kristina
Published: August 2020

Background: The Dyskinesia Impairment Scale (DIS) is a new assessment scale for dystonia and choreoathetosis in children and youth with dyskinetic cerebral palsy. Today, the Burke–Fahn–Marsden Dystonia Rating Scale (BFM) is mostly used to assess dystonia in children with inherited dystonia. The aim of this study was to assess reliability and validity of the DIS in children and youth with inherited or idiopathic dystonia. Methods: Reliability was measured by (1) the intraclass correlation coefficients (ICCs) for inter-rater and test-retest reliability, as well as (2) standard error of measurement (SEM) and minimal detectable difference (MDD). For concurrent validity of the DIS-dystonia subscale, the BFM was administered. Results: In total, 11 males and 9 females (median age 16 years and 7 months, range 6 to 24 years) were included. For inter-rater reliability, the ICCs for the DIS total score and the dystonia and choreoathetosis subscale scores were 0.83, 0.87, and 0.71, respectively. For test-retest reliability, the ICCs for the DIS total score and the dystonia and choreoathetosis subscale scores were 0.95, 0.88, and 0.93, respectively. The SEM and MDD for the total DIS were 3.98% and 11.04%, respectively. The Spearman correlation coefficient between the dystonia subscale and the BFM was 0.88 (p < 0.01). Conclusions: Good to excellent inter-rater, test-retest reliability, and validity were found for the total DIS and the dystonia subscale. The choreoathetosis subscale showed moderate inter-rater reliability and excellent test-retest reliability. The DIS may be a promising tool to assess dystonia and choreoathetosis in children and young adults with inherited or idiopathic dystonia.
Adverse Pregnancy Outcomes after Multi-Professional Follow-Up of Women with Systemic Lupus Erythematosus: An Observational Study from a Single Centre in Sweden
Authors: Saleh Muna , Sjöwall Christopher , Strevens Helena , Jönsen Andreas , Bengtsson Anders A. , Compagno Michele
Published: August 2020

While the management of pregnant patients with systemic lupus erythematosus (SLE) has improved over the last decades, the risk of maternal, foetal, and neonatal complications is still substantial. We evaluated the occurrence of adverse pregnancy outcomes (APO) occurring in 2002–2018 among patients with SLE from the catchment area of the Department of Rheumatology in Lund, Sweden. Longitudinal clinical and laboratory data were collected and analysed. Results were stratified according to the sequence of conception. We investigated a total of 59 pregnancies in 28 patients. Prior lupus nephritis was the clinical feature that, in a multivariable regression analysis, displayed the strongest association with APO overall (OR 6.0, p = 0.02). SLE combined with antiphospholipid syndrome (APS) was associated with the risk of miscarriage (OR 3.3, p = 0.04). The positivity of multiple antiphospholipid antibodies (aPL) was associated with APO overall (OR 3.3, p = 0.05). IgG anti-cardiolipin during pregnancy resulted in a higher risk of preterm delivery (OR 6.8, p = 0.03). Hypocomplementaemia was associated with several APO, but only in the first pregnancies. We conclude that, despite the close follow-up provided, a majority of pregnancies resulted in ≥1 APO, but a few of them were severe. Our study confirms the importance of previous lupus nephritis as a main risk factor for APO in patients with SLE.