bluuebunny/crossref_metadata_2025 huggingface dataset:
Modeling Flexural and Compressive Strengths Behaviour of Cement-Grouted Sands Modified with Water Reducer Polymer
Authors: Mahmood Wael , Mohammed Ahmed Salih , Asteris Panagiotis G. , Kurda Rawaz , Armaghani Danial Jahed
Published: January 2022

By using the American Society for Testing and Materials and British Standards standards, the impact of various grading of sand (Five types of sand) on the compressive strength (CS) of the cement grout (CG) treated with water reducer polymer is investigated. The properties of CG treated with polymer up to 0.16 % of cement weight were investigated and quantified in both fresh and hardened states. The water to cement ratio (w/c) was reduced by 21.9% to 54.1%, and the CG flow time was retained between 18 and 23 s. The highest compression strength was achieved at seven and 28 days for the cement-grouted sand using the coarser-graded sand than finer-graded sand at low w/c ranged between 0.50 and 0.53. The highest compression strength was obtained at high w/c for the cement grout mixed with the fine-grained sands compared to coarse-grained sands. Adding water reducer polymer enhances the compressive strength (σpc) and cylindrical compressive strength (σcc) by 113% to 577% and 53% to 459%, depending on mix proportion and curing period. An amorphous gel fills the porous places between the cement particles were formed when the CG was treated with water reducer polymer, which reduces voids, increases porosity, and increases the cement’s dry density; as a result, the CS of the CG increases significantly. To evaluate the CS of CG with different grain sizes, w/c, percentage of polymer, and curing age, linear and nonlinear techniques were used. according to the bs standard, the CS of the CG produced was 71% higher than that of the identical mix produced according to the ASTM standard. Compared to the other sands, the cement grout produced with finer sand grading had the maximum flexural strength at all testing ages.
Skeleton Motion Recognition Based on Multi-Scale Deep Spatio-Temporal Features
Authors: Hu Kai , Ding Yiwu , Jin Junlan , Weng Liguo , Xia Min
Published: January 2022

In the task of human motion recognition, the overall action span is changeable, and there may be an inclusion relationship between action semantics. This paper proposes a novel multi-scale time sampling module and a deep spatiotemporal feature extraction module, which strengthens the receptive field of the feature map and strengthens the extraction of spatiotemporal-related feature information via the network. We study and compare the performance of three existing multi-channel fusion methods to improve the recognition accuracy of the network on the open skeleton recognition dataset. In this paper, several groups of comparative experiments are carried out on two public datasets. The experimental results show that compared with the classical 2s-AGCN algorithm, the accuracy of the algorithm proposed in this paper shows an improvement of 1% on the Kinetics dataset and 0.4% and 1% on the two evaluating indicators of the NTU-RGB+D dataset, respectively.
Glass as a Fine Art Medium: Brief History and the Role of Adriano Berengo as a Fine Art Glass Impresario
Authors: Bialek Goshka
Published: January 2022

This article explores the role of glass as a medium in the fine arts rather than as a craft form. It includes a short history of glass as an art medium, the development of glass technologies and their application in the field of fine art. It reflects the distinctiveness of glass as a sculptural medium due to its optical properties and transparency; glass’s inherent characteristics create the unique possibility of using the space both outside and inside a solid object. This article, furthermore, demonstrates the importance of specific individuals in bringing glass as a fine art medium to the fore, in particular Adriano Berengo. Berengo proves exceptional in promoting glass in the field of fine arts and has been particularly effective in encouraging well-known artists to experiment with it as a medium. The article discusses the impact of his efforts to establish cooperation with great names from all over the world, from Ai Weiwei to Tony Cragg and from Jaume Plensa to César, who have passed through Adriano Berengo’s studio.
Assessment of 13 Gridded Precipitation Datasets for Hydrological Modeling in a Mountainous Basin
Authors: Hafizi Hamed , Sorman Ali Arda
Published: January 2022

Precipitation measurement with high spatial and temporal resolution over highly elevated and complex terrain in the eastern part of Turkey is an essential task to manage the water structures in an optimum manner. The objective of this study is to evaluate the consistency and hydrologic utility of 13 Gridded Precipitation Datasets (GPDs) (CPCv1, MSWEPv2.8, ERA5, CHIRPSv2.0, CHIRPv2.0, IMERGHHFv06, IMERGHHEv06, IMERGHHLv06, TMPA-3B42v7, TMPA-3B42RTv7, PERSIANN-CDR, PERSIANN-CCS, and PERSIANN) over a mountainous test basin (Karasu) at a daily time step. The Kling-Gupta Efficiency (KGE), including its three components (correlation, bias, and variability ratio), and the Nash-Sutcliffe Efficiency (NSE) are used for GPD evaluation. Moreover, the Hanssen-Kuiper (HK) score is considered to evaluate the detectability strength of selected GPDs for different precipitation events. Precipitation frequencies are evaluated considering the Probability Density Function (PDF). Daily precipitation data from 23 meteorological stations are provided as a reference for the period of 2015–2019. The TUW model is used for hydrological simulations regarding observed discharge located at the outlet of the basin. The model is calibrated in two ways, with observed precipitation only and by each GPD individually. Overall, CPCv1 shows the highest performance (median KGE; 0.46) over time and space. MSWEPv2.8 and CHIRPSv2.0 deliver the best performance among multi-source merging datasets, followed by CHIRPv2.0, whereas IMERGHHFv06, PERSIANN-CDR, and TMPA-3B42v7 show poor performance. IMERGHHLv06 is able to present the best performance (median KGE; 0.17) compared to other satellite-based GPDs (PERSIANN-CCS, PERSIANN, IMERGHHEv06, and TMPA-3B42RTv7). ERA5 performs well both in spatial and temporal validation compared to satellite-based GPDs, though it shows low performance in producing a streamflow simulation. Overall, all gridded precipitation datasets show better performance in generating streamflow when the model is calibrated by each GPD separately.
Differential Expression and Localization of EHBP1L1 during the First Wave of Rat Spermatogenesis Suggest Its Involvement in Acrosome Biogenesis
Authors: Venditti Massimo , Minucci Sergio
Published: January 2022

The identification and characterization of new proteins involved in spermatogenesis is fundamental, considering that good-quality gametes are basic in ensuring proper reproduction. Here, we further analyzed the temporal and spatial localization during the first spermatogenic wave of rat testis of EHBP1L1, which is involved in vesicular trafficking due to the CH and bMERB domains, which bind to actin and Rab8/10, respectively. Western blot and immunofluorescence analyses showed that EHBP1L1 protein expression started at 21 days post-partum (dpp) concomitantly with the appearance of primary spermatocytes (I SPC). In subsequent stages, EHBP1L1 specifically localized together with actin in the perinuclear cytoplasm close to the acrosomal and Golgian regions of spermatids (SPT) during the different phases of acrosome biogenesis (AB). Moreover, it was completely absent in elongated SPT and in mature spermatozoa, suggesting that its role was completed in previous stages. The combined data, also supported by our previous report demonstrating that EHBP1L1 mRNA was expressed by primary (I) and secondary (II) SPC, lead us to hypothesize its specific role during AB. Although these results are suggestive, further studies are needed to better clarify the underlying molecular mechanisms of AB, with the aim to use EHBP1L1 as a potential new marker for spermatogenesis.
Oral Microbiome, Oral Health and Systemic Health: A Multidirectional Link
Authors: Varoni Elena Maria , Rimondini Lia
Published: January 2022

The oral cavity can be regarded as the mirror of systemic health, since many systemic diseases may have manifestations in the oral cavity, as in the case, among oral, potentially malignant disorders, of lupus erythematosus oral lichenoid lesions, and, vice-versa, oral diseases may affect systemic health, impairing patient’s nutrition and wellbeing, reducing the quality of life and increasing stress and anxiety [...]
Elevated Plasma Apurinic/Apyrimidinic Endonuclease 1/Redox Effector Factor-1 Levels in Refractory Kawasaki Disease
Authors: Lee Yu-Ran , Bae Eun Young , Kil Hong Ryang , Jeon Byeong-Hwa , Kim Geena
Published: January 2022

Kawasaki disease (KD) refers to systemic vasculitis of medium-sized vessels accompanied by fever. The multifunctional protein apurinic/apyrimidinic endonuclease-1/redox factor-1 (APE1/Ref-1) is a new biomarker for vascular inflammation. Here, we investigated the association between APE1/Ref-1 and KD. Three groups, including 32 patients with KD (KD group), 33 patients with fever (Fever group), and 19 healthy individuals (Healthy group), were prospectively analyzed. APE1/Ref-1 levels were measured, and the clinical characteristics of KD were evaluated. The mean age of all patients was 2.7 ± 1.8 years, but the Healthy group participants were older than the other participants. Fever duration was longer in the KD group than in the fever group. APE1/Ref-1 levels were significantly higher in the KD group (p = 0.004) than in the other two groups, but there was no difference between the healthy and fever groups. APE1/Ref-1 levels did not differ according to fever duration or coronary arterial lesion but were higher in refractory KD cases than in non-refractory cases. APE1/Ref-1 levels were significantly higher during the acute phase of KD. We propose that APE1/Ref-1 could be a beneficial biological marker for the diagnosis and prognosis of KD, especially in refractory KD.
Burnout, Depression, and Anxiety Levels among Healthcare Workers Serving Children with Autism Spectrum Disorder
Authors: Alenezi Shuliweeh , Almadani Ahmad , Al Tuwariqi Maram , Alzahrani Fahad , Alshabri Meshari , Khoja Mohammed , Al Dakheel Khalid , Alghalayini Khalil , Alkadi Norah , Aljebreen Shahad , Alzahrani Razan
Published: January 2022

Burnout in healthcare workers (HCWs) is defined as a state of emotional, physical, and mental exhaustion that results from unmanaged, excessive, and long-term workplace stressors. This study aims to assess the prevalence of burnout and the levels of anxiety and depression among HCWs who primarily work with children who have autism spectrum disorder (ASD). A quantitative cross-sectional survey was conducted utilizing the Arabic version of the Maslach Burnout Inventory (MBI), Areas of Worklife Survey (AWS), Patient Health Questionnaire for Generalized Anxiety Disorder (GAD-7), and Patient Health Questionnaire for Depression (PHQ-9). Among the 381 participants working in autism centers, the majority were young Saudi females (326) working full-time as specialists in the private sector with less than five years of experience. The HCWs’ overall mean scores on the three Maslach Burnout Inventory (MBI) subscales: emotional exhaustion (EE), depersonalization (DP), and personal accomplishment (PA) were 62%, 23.7%, and 76.5%, respectively. A total of 51.4% of HCWs reported moderate to high anxiety levels on GAD-7, and 47.8% showed moderate to very high levels of depression on PHQ-9. The mean perceived EE converged significantly but negatively on their overall mean perceived satisfaction with AWS (p-value < 0.001), demonstrating that greater emotional fatigue predicts less satisfaction with their work. The PA scores correlated significantly and positively with their overall mean satisfaction with their AWS score (p-value < 0.001). Considering sociodemographic variables, HCWs aged between 20–29 years have significantly lower mean PA scores than HCWs aged thirty and older (p = 0.007). Also, male HCWs perceived significantly higher work-related DP than females. More research is required to determine the nature of variables that contribute to burnout, depression, and anxiety in HCWs helping children with ASD.
Race, Gender, and the U.S. Presidency: A Comparison of Implicit and Explicit Biases in the Electorate
Authors: Calvert Gemma Anne , Evans Geoffrey , Pathak Abhishek
Published: January 2022

Recent U.S. elections have witnessed the Democrats nominating both black and female presidential candidates, as well as a black and female vice president. The increasing diversity of the U.S. political elite heightens the importance of understanding the psychological factors influencing voter support for, or opposition to, candidates of different races and genders. In this study, we investigated the relative strength of the implicit biases for and against hypothetical presidential candidates that varied by gender and race, using an evaluative priming paradigm on a broadly representative sample of U.S. citizens (n = 1076). Our main research question is: Do measures of implicit racial and gender biases predict political attitudes and voting better than measures of explicit prejudice? We find that measures of implicit bias are less strongly associated with political attitudes and voting than are explicit measures of sexist attitudes and modern racism. Moreover, once demographic characteristics and explicit prejudice are controlled statistically, measures of implicit bias provide little incremental predictive validity. Overall, explicit prejudice has a far stronger association with political preferences than does implicit bias.
Unknown Suns: László Hudec, Antonin Raymond and the Rising of a Modern Architecture for Eastern Asia
Authors: Anghel Anamaria Andreea , Cabeza-Lainez Joseph , Xu Yingying
Published: January 2022

The purpose of this article is to disclose the strenuous efforts of László Hudec in China and Antonin Raymond in Japan and India to create a modern architectural stance by heralding an incipient space syntax. At the turn of the 19th century, for dynastic, political and economic reasons, Eastern Asia had very little modern architecture. It is a surprising fact that, out of happenstance, two European architects, Antonin Raymond and László Hudec, had to intervene to remedy this situation, to the point of becoming 20th century icons in Japan and China. Their fruitful careers spanned over thirty years and included locations like Tamil Nadu and the Philippines. The oriental territories were not an easy ground for the bold architectural achievements that they produced. Despite faraway strangeness and uncountable personal losses, in revolutions and wars, which eventually forced them both to leave for the United States of America and never to return, they were successful in the manner of establishing a broad avenue for modern Asian architecture which is still recognizable today thanks to their systematic approach. However, theirs is an endangered heritage and the intention of this article is to offer a just remembrance of the way in which such actions could be performed, how they predated by many years a syntactic approach to architectural composition and why their legacy should be preserved.
Hepatocellular Carcinoma Is a Natural Target for Adeno-Associated Virus (AAV) 2 Vectors
Authors: Meumann Nadja , Schmithals Christian , Elenschneider Leroy , Hansen Tanja , Balakrishnan Asha , Hu Qingluan , Hook Sebastian , Schmitz Jessica , Bräsen Jan Hinrich , Franke Ann-Christin , Olarewaju Olaniyi , Brandenberger Christina , Talbot Steven R. , Fangmann Josef , Hacker Ulrich T. , Odenthal Margarete , Ott Michael , Piiper Albrecht , Büning Hildegard
Published: January 2022

Although therapeutic options are gradually improving, the overall prognosis for patients with hepatocellular carcinoma (HCC) is still poor. Gene therapy-based strategies are developed to complement the therapeutic armamentarium, both in early and late-stage disease. For efficient delivery of transgenes with antitumor activity, vectors demonstrating preferred tumor tropism are required. Here, we report on the natural tropism of adeno-associated virus (AAV) serotype 2 vectors for HCC. When applied intravenously in transgenic HCC mouse models, similar amounts of vectors were detected in the liver and liver tumor tissue. In contrast, transduction efficiency, as indicated by the level of transgene product, was moderate in the liver but was elevated up to 19-fold in mouse tumor tissue. Preferred transduction of HCC compared to hepatocytes was confirmed in precision-cut liver slices from human patient samples. Our mechanistic studies revealed that this preference is due to the improved intracellular processing of AAV2 vectors in HCC, resulting, for example, in nearly 4-fold more AAV vector episomes that serve as templates for gene transcription. Given this background, AAV2 vectors ought to be considered to strengthen current—or develop novel—strategies for treating HCC.
Multimodal Management of Grade 1 and 2 Pancreatic Neuroendocrine Tumors
Authors: Marchese Ugo , Gaillard Martin , Pellat Anna , Tzedakis Stylianos , Abou Ali Einas , Dohan Anthony , Barat Maxime , Soyer Philippe , Fuks David , Coriat Romain
Published: January 2022

Pancreatic neuroendocrine tumors (p-NETs) are rare tumors with a recent growing incidence. In the 2017 WHO classification, p-NETs are classified into well-differentiated (i.e., p-NETs grade 1 to 3) and poorly differentiated neuroendocrine carcinomas (i.e., p-NECs). P-NETs G1 and G2 are often non-functioning tumors, of which the prognosis depends on the metastatic status. In the localized setting, p-NETs should be surgically managed, as no benefit for adjuvant chemotherapy has been demonstrated. Parenchymal sparing resection, including both duodenum and pancreas, are safe procedures in selected patients with reduced endocrine and exocrine long-term dysfunction. When the p-NET is benign or borderline malignant, this surgical option is associated with low rates of severe postoperative morbidity and in-hospital mortality. This narrative review offers comments, tips, and tricks from reviewing the available literature on these different options in order to clarify their indications. We also sum up the overall current data on p-NETs G1 and G2 management.
Applications of Extracellular Vesicles in Triple-Negative Breast Cancer
Authors: St-Denis-Bissonnette Frederic , Khoury Rachil , Mediratta Karan , El-Sahli Sara , Wang Lisheng , Lavoie Jessie R.
Published: January 2022

Triple-negative breast cancer (TNBC) is the most aggressive and refractory subtype of breast cancer, often occurring in younger patients with poor clinical prognosis. Given the current lack of specific targets for effective intervention, the development of better treatment strategies remains an unmet medical need. Over the last decade, the field of extracellular vesicles (EVs) has grown tremendously, offering immense potential for clinical diagnosis/prognosis and therapeutic applications. While TNBC-EVs have been shown to play an important role in tumorigenesis, chemoresistance and metastasis, they could be repurposed as potential biomarkers for TNBC diagnosis and prognosis. Furthermore, EVs from various cell types can be utilized as nanoscale drug delivery systems (NDDS) for TNBC treatment. Remarkably, EVs generated from specific immune cell subsets have been shown to delay solid tumour growth and reduce tumour burden, suggesting a new immunotherapy approach for TNBC. Intrinsically, EVs can cross the blood–brain barrier (BBB), which holds great potential to treat the brain metastases diagnosed in one third of TNBC patients that remains a substantial clinical challenge. In this review, we present the most recent applications of EVs in TNBC as diagnostic/prognostic biomarkers, nanoscale drug delivery systems and immunotherapeutic agents, as well as discuss the associated challenges and future directions of EVs in cancer immunotherapy.
Laser Interstitial Thermal Therapy for Posterior Fossa Lesions: A Systematic Review and Analysis of Multi-Institutional Outcomes
Authors: Sabahi Mohammadmahdi , Bordes Stephen J. , Najera Edinson , Mohammadi Alireza M. , Barnett Gene H. , Adada Badih , Borghei-Razavi Hamid
Published: January 2022

Background: Laser interstitial thermal therapy (LITT) has emerged as a treatment option for deep-seated primary and metastatic brain lesions; however, hardly any data exist regarding LITT for lesions of the posterior fossa. Methods: A quantitative systematic review was performed. Article selection was performed by searching MEDLINE (using PubMed), Scopus, and Cochrane electronic bibliographic databases. Inclusion criteria were studies assessing LITT on posterior fossa tumors. Results: 16 studies comprising 150 patients (76.1% female) with a mean age of 56.47 years between 2014 and 2021 were systematically reviewed for treatment outcomes and efficacy. Morbidity and mortality data could be extracted for 131 of the 150 patients. Death attributed to treatment failure, disease progression, recurrence, or postoperative complications occurred in 6.87% (9/131) of the pooled sample. Procedure-related complications, usually including new neurologic deficits, occurred in approximately 14.5% (19/131) of the pooled sample. Neurologic deficits improved with time in most cases, and 78.6% (103/131) of the pooled sample experienced no complications and progression-free survival at the time of last follow-up. Conclusions: LITT for lesions of the posterior fossa continues to show promising data. Future clinical cohort studies are required to further direct treatment recommendations.
Horizons in Asymmetric Organocatalysis: En Route to the Sustainability and New Applications
Authors: Ardevines Sandra , Marqués-López Eugenia , Herrera Raquel P.
Published: January 2022

Nowadays, the development of new enantioselective processes is highly relevant in chemistry due to the relevance of chiral compounds in biomedicine (mainly drugs) and in other fields, such as agrochemistry, animal feed, and flavorings. Among them, organocatalytic methods have become an efficient and sustainable alternative since List and MacMillan pioneering contributions were published in 2000. These works established the term asymmetric organocatalysis to label this area of research, which has grown exponentially over the last two decades. Since then, the scientific community has attended to the discovery of a plethora of organic reactions and transformations carried out with excellent results in terms of both reactivity and enantioselectivity. Looking back to earlier times, we can find in the literature a few examples where small organic molecules and some natural products could act as effective catalysts. However, with the birth of this type of catalysis, new chemical architectures based on amines, thioureas, squaramides, cinchona alkaloids, quaternary ammonium salts, carbenes, guanidines and phosphoric acids, among many others, have been developed. These organocatalysts have provided a broad range of activation modes that allow privileged interactions between catalysts and substrates for the preparation of compounds with high added value in an enantioselective way. Here, we briefly cover the history of this chemistry, from our point of view, including our beginnings, how the field has evolved during these years of research, and the road ahead.
Cr(III) Complexes Bearing a β-Ketoimine Ligand for Olefin Polymerization: Are There Differences between Coordinative and Covalent Bonding?
Authors: Amodio Alessia , Zanchin Giorgia , De Stefano Fabio , Piovano Alessandro , Palucci Benedetta , Guiotto Virginia , Di Girolamo Rocco , Leone Giuseppe , Groppo Elena
Published: January 2022

β-ketoimines are extensively applied for the synthesis of organometallic complexes intended as (pre)catalysts for a variety of chemical transformations. We were interested in the synthesis of two Cr complexes bearing a simple bidentate β-ketoimine (L), with different ligand binding modes, as well as their application as a precatalyst in the polymerization of olefins. Complex 1 (L2CrCl3) was obtained by direct reaction of L with CrCl3(THF)3, while, for the synthesis of complex 2 (LCrCl2), the ligand was first deprotonated with nBuLi, giving the β-ketoiminato ligand L─Li+, and then reacted with CrCl3(THF)3. Characterization of the complexes proved that the Cr(III) ion is coordinatively bonded to L in 1, while it is covalently bonded to L in 2. The complexes were then used as precatalysts for the polymerization of ethylene and various cyclic olefins. Upon activation with methylaluminoxane, both the complexes exhibited poor activity in the polymerization of ethylene, whilst they exhibit good productivity in the polymerization of cyclic olefins, affording semicrystalline oligomers, without a significant difference between 1 and 2. To gain more insight, we investigated the reaction of the complexes with the Al-cocatalyst by IR and UV-Vis spectroscopies. The results proved that, in case of 1, the Al-activator deprotonates the ligand, bringing to the formation of an active species analogous to that of 2.
Implication of Adult Hippocampal Neurogenesis in Alzheimer’s Disease and Potential Therapeutic Approaches
Authors: Essa Hesham , Peyton Lee , Hasan Whidul , León Brandon Emanuel , Choi Doo-Sup
Published: January 2022

Alzheimer’s disease is the most common neurodegenerative disease, affecting more than 6 million US citizens and representing the most prevalent cause for dementia. Neurogenesis has been repeatedly reported to be impaired in AD mouse models, but the reason for this impairment remains unclear. Several key factors play a crucial role in AD including Aβ accumulation, intracellular neurofibrillary tangles accumulation, and neuronal loss (specifically in the dentate gyrus of the hippocampus). Neurofibrillary tangles have been long associated with the neuronal loss in the dentate gyrus. Of note, Aβ accumulation plays an important role in the impairment of neurogenesis, but recent studies started to shed a light on the role of APP gene expression on the neurogenesis process. In this review, we will discuss the recent approaches to neurogenesis in Alzheimer disease and update the development of therapeutic methods.
Glucose Regulates m6A Methylation of RNA in Pancreatic Islets
Authors: Bornaque Florine , Delannoy Clément Philippe , Courty Emilie , Rabhi Nabil , Carney Charlène , Rolland Laure , Moreno Maeva , Gromada Xavier , Bourouh Cyril , Petit Pauline , Durand Emmanuelle , Pattou François , Kerr-Conte Julie , Froguel Philippe , Bonnefond Amélie , Oger Frédérik , Annicotte Jean-Sébastien
Published: January 2022

Type 2 diabetes is characterized by chronic hyperglycemia associated with impaired insulin action and secretion. Although the heritability of type 2 diabetes is high, the environment, including blood components, could play a major role in the development of the disease. Amongst environmental effects, epitranscriptomic modifications have been recently shown to affect gene expression and glucose homeostasis. The epitranscriptome is characterized by reversible chemical changes in RNA, with one of the most prevalent being the m6A methylation of RNA. Since pancreatic β cells fine tune glucose levels and play a major role in type 2 diabetes physiopathology, we hypothesized that the environment, through variations in blood glucose or blood free fatty acid concentrations, could induce changes in m6A methylation of RNAs in pancreatic β cells. Here we observe a significant decrease in m6A methylation upon high glucose concentration, both in mice and human islets, associated with altered expression levels of m6A demethylases. In addition, the use of siRNA and/or specific inhibitors against selected m6A enzymes demonstrate that these enzymes modulate the expression of genes involved in pancreatic β-cell identity and glucose-stimulated insulin secretion. Our data suggest that environmental variations, such as glucose, control m6A methylation in pancreatic β cells, playing a key role in the control of gene expression and pancreatic β-cell functions. Our results highlight novel causes and new mechanisms potentially involved in type 2 diabetes physiopathology and may contribute to a better understanding of the etiology of this disease.
STAT3 Is the Master Regulator for the Forming of 3D Spheroids of 3T3-L1 Preadipocytes
Authors: Ohguro Hiroshi , Ida Yosuke , Hikage Fumihito , Umetsu Araya , Ichioka Hanae , Watanabe Megumi , Furuhashi Masato
Published: January 2022

To elucidate the currently unknown mechanisms responsible for the diverse biological aspects between two-dimensional (2D) and three-dimensional (3D) cultured 3T3-L1 preadipocytes, RNA-sequencing analyses were performed. During a 7-day culture period, 2D- and 3D-cultured 3T3-L1 cells were subjected to lipid staining by BODIPY, qPCR for adipogenesis related genes, including peroxisome proliferator-activated receptor γ (Pparγ), CCAAT/enhancer-binding protein alpha (Cebpa), Ap2 (fatty acid-binding protein 4; Fabp4), leptin, and AdipoQ (adiponectin), and RNA-sequencing analysis. Differentially expressed genes (DEGs) were detected by next-generation RNA sequencing (RNA-seq) and validated by a quantitative reverse transcription–polymerase chain reaction (qRT–PCR). Bioinformatic analyses were performed on DEGs using a Gene Ontology (GO) enrichment analysis and an Ingenuity Pathway Analysis (IPA). Significant spontaneous adipogenesis was observed in 3D 3T3-L1 spheroids, but not in 2D-cultured cells. The mRNA expression of Pparγ, Cebpa, and Ap2 among the five genes tested were significantly higher in 3D spheroids than in 2D-cultured cells, thus providing support for this conclusion. RNA analysis demonstrated that a total of 826 upregulated and 725 downregulated genes were identified as DEGs. GO enrichment analysis and IPA found 50 possible upstream regulators, and among these, 6 regulators—transforming growth factor β1 (TGFβ1), signal transducer and activator of transcription 3 (STAT3), interleukin 6 (IL6), angiotensinogen (AGT), FOS, and MYC—were, in fact, significantly upregulated. Further analyses of these regulators by causal networks of the top 14 predicted diseases and functions networks (IPA network score indicated more than 30), suggesting that STAT3 was the most critical upstream regulator. The findings presented herein suggest that STAT3 has a critical role in regulating the unique biological properties of 3D spheroids that are produced from 3T3-L1 preadipocytes.
Rhenium Perrhenate (188ReO4) Induced Apoptosis and Reduced Cancerous Phenotype in Liver Cancer Cells
Authors: Asadian Samieh , Piryaei Abbas , Gheibi Nematollah , Aziz Kalantari Bagher , Reza Davarpanah Mohamad , Azad Mehdi , Kapustina Valentina , Alikhani Mehdi , Moghbeli Nejad Sahar , Keshavarz Alikhani Hani , Mohamadi Morteza , Shpichka Anastasia , Timashev Peter , Hassan Moustapha , Vosough Massoud
Published: January 2022

Recurrence in hepatocellular carcinoma (HCC) after conventional treatments is a crucial challenge. Despite the promising progress in advanced targeted therapies, HCC is the fourth leading cause of cancer death worldwide. Radionuclide therapy can potentially be a practical targeted approach to address this concern. Rhenium-188 (188Re) is a β-emitting radionuclide used in the clinic to induce apoptosis and inhibit cell proliferation. Although adherent cell cultures are efficient and reliable, appropriate cell-cell and cell-extracellular matrix (ECM) contact is still lacking. Thus, we herein aimed to assess 188Re as a potential therapeutic component for HCC in 2D and 3D models. The death rate in treated Huh7 and HepG2 lines was significantly higher than in untreated control groups using viability assay. After treatment with 188ReO4, Annexin/PI data indicated considerable apoptosis induction in HepG2 cells after 48 h but not Huh7 cells. Quantitative RT-PCR and western blotting data also showed increased apoptosis in response to 188ReO4 treatment. In Huh7 cells, exposure to an effective dose of 188ReO4 led to cell cycle arrest in the G2 phase. Moreover, colony formation assay confirmed post-exposure growth suppression in Huh7 and HepG2 cells. Then, the immunostaining displayed proliferation inhibition in the 188ReO4-treated cells on 3D scaffolds of liver ECM. The PI3-AKT signaling pathway was activated in 3D culture but not in 2D culture. In nude mice, Huh7 cells treated with an effective dose of 188ReO4 lost their tumor formation ability compared to the control group. These findings suggest that 188ReO4 can be a potential new therapeutic agent against HCC through induction of apoptosis and cell cycle arrest and inhibition of tumor formation. This approach can be effectively combined with antibodies and peptides for more selective and personalized therapy.
Emerging Role of cAMP/AMPK Signaling
Authors: Aslam Muhammad , Ladilov Yury
Published: January 2022

The 5′-Adenosine monophosphate (AMP)-activated protein kinase (AMPK) is a natural energy sensor in mammalian cells that plays a key role in cellular and systemic energy homeostasis. At the cellular level, AMPK supports numerous processes required for energy and redox homeostasis, including mitochondrial biogenesis, autophagy, and glucose and lipid metabolism. Thus, understanding the pathways regulating AMPK activity is crucial for developing strategies to treat metabolic disorders. Mounting evidence suggests the presence of a link between cyclic AMP (cAMP) and AMPK signaling. cAMP signaling is known to be activated in circumstances of physiological and metabolic stress due to the release of stress hormones, such as adrenaline and glucagon, which is followed by activation of membrane-bound adenylyl cyclase and elevation of cellular cAMP. Because the majority of physiological stresses are associated with elevated energy consumption, it is not surprising that activation of cAMP signaling may promote AMPK activity. Aside from the physiological role of the cAMP/AMPK axis, numerous reports have suggested its role in several pathologies, including inflammation, ischemia, diabetes, obesity, and aging. Furthermore, novel reports have provided more mechanistic insight into the regulation of the cAMP/AMPK axis. In particular, the role of distinct cAMP microdomains generated by soluble adenylyl cyclase in regulating basal and induced AMPK activity has recently been demonstrated. In the present review, we discuss current advances in the understanding of the regulation of the cAMP/AMPK axis and its role in cellular homeostasis and explore some translational aspects.
Recent Advances in Chemical Sensors for Soil Analysis: A Review
Authors: Nadporozhskaya Marina , Kovsh Ninel , Paolesse Roberto , Lvova Larisa
Published: January 2022

The continuously rising interest in chemical sensors’ applications in environmental monitoring, for soil analysis in particular, is owed to the sufficient sensitivity and selectivity of these analytical devices, their low costs, their simple measurement setups, and the possibility to perform online and in-field analyses with them. In this review the recent advances in chemical sensors for soil analysis are summarized. The working principles of chemical sensors involved in soil analysis; their benefits and drawbacks; and select applications of both the single selective sensors and multisensor systems for assessments of main plant nutrition components, pollutants, and other important soil parameters (pH, moisture content, salinity, exhaled gases, etc.) of the past two decades with a focus on the last 5 years (from 2017 to 2021) are overviewed.
Loss of Health Promoting Bacteria in the Gastrointestinal Microbiome of PICU Infants with Bronchiolitis: A Single-Center Feasibility Study
Authors: Russell Madeleine M. , Leimanis-Laurens Mara L. , Bu Sihan , Kinney Gigi A. , Teoh Shao Thing , McKee Ruth-Anne L. , Ferguson Karen , Winters John W. , Lunt Sophia Y. , Prokop Jeremy W. , Rajasekaran Surender , Comstock Sarah S.
Published: January 2022

The feasibility of gastrointestinal (GI) microbiome work in a pediatric intensive care unit (PICU) to determine the GI microbiota composition of infants as compared to control infants from the same hospital was investigated. In a single-site observational study at an urban quaternary care children’s hospital in Western Michigan, subjects less than 6 months of age, admitted to the PICU with severe respiratory syncytial virus (RSV) bronchiolitis, were compared to similarly aged control subjects undergoing procedural sedation in the outpatient department. GI microbiome samples were collected at admission (n = 20) and 72 h (n = 19) or at time of sedation (n = 10). GI bacteria were analyzed by sequencing the V4 region of the 16S rRNA gene. Alpha and beta diversity were calculated. Mechanical ventilation was required for the majority (n = 14) of study patients, and antibiotics were given at baseline (n = 8) and 72 h (n = 9). Control subjects’ bacterial communities contained more Porphyromonas, and Prevotella (p = 0.004) than those of PICU infants. The ratio of Prevotella to Bacteroides was greater in the control than the RSV infants (mean ± SD—1.27 ± 0.85 vs. 0.61 ± 0.75: p = 0.03). Bacterial communities of PICU infants were less diverse than those of controls with a loss of potentially protective populations.
Effect of UV Radiation on Structural Damage and Tribological Properties of Mo/MoS2-Pb-PbS Composite Films
Authors: Han Cuihong , Li Guolu , Ma Guozheng , Shi Jiadong , Li Zhen , Yong Qingsong , Wang Haidou
Published: January 2022

To investigate ultraviolet (UV) radiation effects on tribological properties of Mo/MoS2-Pb-PbS film, ultraviolet (UV) radiation exposure tests were carried out for 20 h, 40 h, 60 h and 80 h by space UV radiation simulation device developed by our team, which can reach 3 UV radiation intensity. The exposure time in test was equivalent to the radiation of 100 h, 200 h, 300 h and 400 h in the space. Then, the vacuum friction test of Mo/MoS2-Pb-PbS thin film was performed under the 6 N load and 100 r/min, and friction test time of each sample was 20 min. By SEM, TEM, XPS the composition and morphology of Mo/MoS2-Pb-PbS film surface after UV radiation were analyzed. UV radiation could change the microstructure significantly and relative content of S element and MoS2 on the surface of the films decreased, and light mass loss of the films occurred. The tribological properties will also recover with the increase of sliding time, although the friction coefficient fluctuation of the film increased at the starting stage of the friction test. The damage of Mo/MoS2-Pb-PbS under UV irradiation was mainly caused by the volatilization of the enriched S element in the surface layer due to the high temperature heating of UV irradiation.
Computational Analysis of Active and Passive Flow Control for Backward Facing Step
Authors: Moulinos Iosif , Manopoulos Christos , Tsangaris Sokrates
Published: January 2022

The internal steady and unsteady flows with a frequency and amplitude are examined through a backward facing step (expansion ratio 2), for low Reynolds numbers (Re=400, Re=800), using the immersed boundary method. A lower part of the backward facing step is oscillating with the same frequency as the unsteady flow. The effect of the frequency, the amplitude, and the length of this oscillation is investigated. By suitable active control regulation, the recirculation lengths are reduced, and, for a percentage of the time period, no upper wall, negative velocity, region occurs. Moreover, substituting the prescriptively moving surface by a pressure responsive homogeneous membrane, the fluid–structure interaction is examined. We show that, by selecting proper values for the membrane parameters, such as membrane tension and applied external pressure, the upper wall flow separation bubble vanishes, while the lower one diminishes significantly in both the steady and the unsteady cases. Furthermore, for the time varying case, the length fluctuation of the lower wall reversed flow region is fairly contracted. The findings of the study have applications at the control of confined and external flows where separation occurs.
Luminescence Properties of Gd2(MoO4)3 Modified with Sm(III) and Tb(III) for Potential LED Applications
Authors: Tsvetkov Martin , Elenkova Denitsa , Milanova Maria
Published: January 2022

Light-emitting phosphors, doped with lanthanide ions of Tb(III) and Sm(III) of the type Gd1.97−y SmyTb0.03(MoO4)3 (y = 0.01–0.11, step 0.02) and Gd1.95−xSm0.05Tbx(MoO4)3 (x = 0.01–0.09, step 0.02), were synthesized and characterized by X-ray diffraction, UV-Vis spectroscopy, scanning and transmitting electron microscopy (SEM, TEM) as well as photoluminescence spectroscopy. The effect of the doping content of Tb/Sm was followed. The unit cell parameters for Gd1.97−ySmyTb0.03(MoO4)3 and Gd1.95−xSm0.05Tbx(MoO4)3 changed with the increase in the Tb/Sm content. The microstrain values also increased, proposing an increased concentration of defects. The mean particle size was estimated to be approximately 0.6 µm. Based on a Williamson–Hall plot, the size of the crystallites was determined to be in the range of 42–60 nm for modified and pure Gd2(MoO4)3 samples, respectively. The samples excited at 406 nm exhibited characteristic emission lines of Sm (485, 555, 646 nm). The host material Gd2(MoO4)3 emission in visible light was explained by the crystal structure defects, namely, oxygen vacancies. The CIE x/y color coordinates of the phosphors were determined and the related points were located in the green-yellow/pale yellow region of the visible light. The excited state lifetimes were determined for both groups of the samples, showing values in the millisecond range and indicating the samples as promising phosphors.
Study on the Relationship between Richness and Morphological Diversity of Higher Taxa in the Darkling Beetles (Coleoptera: Tenebrionidae)
Authors: Cheng Liangxue , Tong Yijie , Zhao Yuchen , Sun Zhibin , Wang Xinpu , Ma Fangzhou , Bai Ming
Published: January 2022

Many studies have found that the correlation between species richness (SR) and morphological diversity (MD) is positive, but the correlation degree of these parameters is not always consistent due to differences in categories and various ecological factors in the living environment. Based on this, related studies have revealed the good performance of using higher taxa in biodiversity research, not only by shifting the testing group scale from local communities to worldwide datasets but also by adding different taxonomic levels, such as the genus level. However, it remains unclear whether this positive correlation can also be applied to other categories or groups. Here, we evaluated the applicability of higher taxa in the biodiversity study of darkling beetles by using 3407 species (9 subfamilies, 89 tribes, and 678 genera), based on the correlation between taxa richness and morphological diversity in the tribe/genus/species. In addition, the continuous features prevalent in the tenebrionids, pronotum and elytron, were selected, and the morphological diversity of various groups was obtained by the geometric morphometric approach to quantify the morphologic information of features. This study found that genus/species richness in subfamilies Pimelinae and Stenochiinae was positively correlated with the change trend of MD, and the correlation between the MD of elytron and taxa richness gradually decreased from the tribe-level to the genus-level to the species-level test. The results confirm the stable morphology and simple function of the elytron and the applicability of tribe level in biodiversity research.
A Deep Reinforcement Learning Strategy Combining Expert Experience Guidance for a Fruit-Picking Manipulator
Authors: Liu Yuqi , Gao Po , Zheng Change , Tian Lijing , Tian Ye
Published: January 2022

When using deep reinforcement learning algorithms for path planning of a multi-DOF fruit-picking manipulator in unstructured environments, it is much too difficult for the multi-DOF manipulator to obtain high-value samples at the beginning of training, resulting in low learning and convergence efficiency. Aiming to reduce the inefficient exploration in unstructured environments, a reinforcement learning strategy combining expert experience guidance was first proposed in this paper. The ratios of expert experience to newly generated samples and the frequency of return visits to expert experience were studied by the simulation experiments. Some conclusions were that the ratio of expert experience, which declined from 0.45 to 0.35, was more effective in improving learning efficiency of the model than the constant ratio. Compared to an expert experience ratio of 0.35, the success rate increased by 1.26%, and compared to an expert experience ratio of 0.45, the success rate increased by 20.37%. The highest success rate was achieved when the frequency of return visits was 15 in 50 episodes, an improvement of 31.77%. The results showed that the proposed method can effectively improve the model performance and enhance the learning efficiency at the beginning of training in unstructured environments. This training method has implications for the training process of reinforcement learning in other domains.
Cellulose Isolation from Tomato Pomace Pretreated by High-Pressure Homogenization
Authors: Pirozzi Annachiara , Ferrari Giovanna , Donsì Francesco
Published: January 2022

This work proposes a biorefinery approach for the utilization of agri-food residues, such as tomato pomace (TP), through combining chemical hydrolysis with high-pressure homogenization (HPH), aiming to achieve the isolation of cellulose with tailored morphological properties from underused lignocellulose feedstocks, along with the valorization of the value-added compounds contained in the biomass. Cellulose was isolated from TP using sequential chemical hydrolysis in combination with mechanical pretreatment through HPH. The chemical and structural features of cellulose isolated from TP pretreated by HPH were compared with cellulose isolated from untreated TP through light scattering for particle size distribution, optical and scanning electron microscopy, and Fourier-transform infrared spectroscopy (FT-IR) analysis. HPH pretreatment (80 MPa, 10 passes) not only promoted a slight increase in the yield of cellulose extraction (+9%) but contributed to directly obtaining defibrillated cellulose particles, characterized by smaller irregular domains containing elongated needle-like fibers. Moreover, the selected mild chemical process produced side streams rich in bioactive molecules, evaluated in terms of total phenols and reducing activity. The liquors recovered from acid hydrolysis of TP exhibited a higher biological activity than those obtained through a conventional extraction (80% v/v acetone, 25 °C, 24 h at 180 rpm).