bluuebunny/crossref_metadata_2025 huggingface dataset:
Decoding diets: insights on ultra-processed food consumption among Lebanese adults from the updated LEBANese natiONal food consumption survey (LEBANON-FCS)
Authors: Khattar Maroun , Tzenios Nikolaos , Antar Esraa , Malli Dana ,   The Adults-Lebanon-FCS Group, Hoteit Maha
Published: December 2024

Introduction

Ultra-processed foods are evident to play a role in the development of nutrition-related non-communicable diseases (NR-NCDs). There’s a scarcity of data in the Eastern Mediterranean Region (EMR) regarding ultra-processed food consumption, which highlights the need for such data in this region that is witnessing a nutrition transition. This study was conducted to assess the dietary pattern of Lebanese adults according to different degrees of food processing.

Methods

A cross-sectional study involving a nationally representative sample (n = 444) of Lebanese adults (18–64 years) was conducted over the course of 5 months. A validated food frequency questionnaire and two non-consecutive 24-h recalls were used to collect the dietary intake. Sociodemographic and medical characteristics were collected using a validated questionnaire. Anthropometric measurements were taken by trained dietitians. Food items were classified according to the NOVA food classification system, and the percent contribution of every type of processing to the energy intake was calculated.

Results

Ultra-processed foods contributed to the highest percent of energy intake in the sample (46.7%), followed by unprocessed and minimally processed foods (39.6%). Being male, and younger increased the odds of higher ultra-processed food intake; being employed and food secure increased the odds of a higher NOVA-UPF score. Compared with participants in Beirut, participants residing in North Lebanon and Akkar had higher odds of having a high UPF intake and lower odds of having a high NOVA-UPF score. The three most contributing food items to energy intake were ultra-processed (breads and breakfast cereals, fast foods, sweets and desserts). Compared to the unprocessed and minimally processed food diet fraction, the ultra-processed food diet fraction was significantly higher in sodium and thiamin and lower in proteins, fiber, and essential vitamins and minerals such as vitamins A, D, B12, folate, iron, calcium, and magnesium.

Conclusion

UPF intake was the main contributor to TEI, and the intake was considerably higher among those who reported having renal disease, younger adults, and males. In comparison to the minimally processed diet fraction, the UPF diet fraction was found to have considerably greater levels of sodium and thiamin and lower levels of proteins, fiber, and essential vitamins and minerals. The study findings call for public health policies and interventions to encourage the consumption of minimally processed foods and decrease the consumption of ultra-processed foods, especially sweets and sweetened beverages, which are especially problematic.
Rare giant epithelioid inflammatory myofibroblastic sarcoma of the abdominal cavity in a child: a case report and review of the literature
Authors: Li Jinzhou , Su Haixing , Zhang Sheng , Chen Xianyun , Hou Chongzhi , Cheng Tao
Published: December 2024

Epithelioid inflammatory myofibroblastic sarcoma (EIMS) is a distinct subtype of inflammatory myofibroblastoma tumor (IMT) that is recognized as a rare malignant tumor characterized by anaplastic lymphoma kinase (ALK) positivity, significant aggressiveness, treatment challenges, and a poor prognosis. We report on the case of an 8-year-old boy presenting with abdominal pain and vomiting. Computed tomography (CT) of the abdomen revealed a large tumor, and the pathology results following a biopsy confirmed the diagnosis of EIMS. The patient underwent radical tumor resection, and genetic testing identified the presence of the RANBP2–ALK fusion. To our knowledge, this represents the largest pediatric case of abdominal EIMS documented in the literature. Currently, there is no standard therapy for EIMS; however, existing studies advocate for the use of ALK tyrosine kinase inhibitors (TKIs) in its treatment. This case was reported to be in remission following treatment with crizotinib, thereby contributing to the understanding of the specific pathology of EIMS and facilitating accurate diagnosis and targeted therapy.
Case report: CAR-T therapy demonstrated safety and efficacy in relapsed/refractory diffuse large B-cell lymphoma patients complicated with hepatitis B-related cirrhosis
Authors: Kong Danqing , Ping Nana , Zhu Qian , Zhang Xiao , Li Junhong , Zou Rui , Wu Depei , Jin Zhengming , Qu Changju
Published: December 2024

Chimeric antigen receptor T-cell (CAR-T) therapy has demonstrated both efficacy and safety in relapsed/refractory diffuse large B-cell lymphoma (DLBCL) patients infected with hepatitis B virus (HBV). However, its applicability in individuals with liver cirrhosis remains largely unexplored due to the potential for unpredictable complications. Here, we report three cases (P1, P2, and P3) of relapsed/refractory DLBCL with HBV-related cirrhosis treated with CAR-T cell infusion. P1 and P2 received CAR-T cell infusion following a conditioning regimen of fludarabine and cyclophosphamide (FC) for lymphodepletion, while P3 received the SEAM (semustine, etoposide, cytarabine, and melphalan) regimen and autologous stem cell transplantation bridging CAR-T cell infusion. P1 and P2 achieved rapid complete remission (CR), whereas P3 initially exhibited stable disease a month after CAR-T infusion and subsequently achieved CR after local radiation salvage therapy and lenalidomide maintenance. With a median follow-up of 42 months after CAR-T, the progression-free survival rate was 100%. Notably, during follow-up, these patients experienced complications associated with cirrhosis, including endoscopic variceal bleeding, HBV reactivation, or the diagnosis of hepatic malignancy. Our findings suggest that CAR-T therapy is applicable and effective for the treatment of DLBCL patients with HBV-related cirrhosis, albeit necessitating monitoring for potential hepatic complications.
The role of CYP-sEH derived lipid mediators in regulating mitochondrial biology and cellular senescence: implications for the aging heart
Authors: Yousef Ala , Fang Liye , Heidari Mobina , Kranrod Joshua , Seubert John M.
Published: December 2024

Cellular senescence is a condition characterized by stable, irreversible cell cycle arrest linked to the aging process. The accumulation of senescent cells in the cardiac muscle can contribute to various cardiovascular diseases (CVD). Telomere shortening, epigenetic modifications, DNA damage, mitochondrial dysfunction, and oxidative stress are known contributors to the onset of cellular senescence in the heart. The link between mitochondrial processes and cellular senescence contributed to the age-related decline in cardiac function. These include changes in mitochondrial functions and behaviours that arise from various factors, including impaired dynamics, dysregulated biogenesis, mitophagy, mitochondrial DNA (mtDNA), reduced respiratory capacity, and mitochondrial structural changes. Thus, regulation of mitochondrial biology has a role in cellular senescence and cardiac function in aging hearts. Targeting senescent cells may provide a novel therapeutic approach for treating and preventing CVD associated with aging. CYP epoxygenases metabolize N-3 and N-6 polyunsaturated fatty acids (PUFA) into epoxylipids that are readily hydrolyzed to diol products by soluble epoxide hydrolase (sEH). Increasing epoxylipids levels or inhibition of sEH has demonstrated protective effects in the aging heart. Evidence suggests they may play a role in cellular senescence by regulating mitochondria, thus reducing adverse effects of aging in the heart. In this review, we discuss how mitochondria induce cellular senescence and how epoxylipids affect the senescence process in the aged heart.
Shikonin suppresses proliferation of osteosarcoma cells by inducing ferroptosis through promoting Nrf2 ubiquitination and inhibiting the xCT/GPX4 regulatory axis
Authors: Huang Rui , Chu Dawei , Shi Jiandang , Xu Ruiqing , Wang Kun
Published: December 2024

Osteosarcoma (OS) is a prevalent primary malignant bone tumor which lacks effective therapeutic interventions. Ferroptosis is a new form of programmed cell death characterized by iron-dependent accumulation of lethal lipid oxidation, which provides a potential alternative intervene for the OS treatment. Shikonin is the major bioactive component extracted from the roots of lithospermum erythrorhizon which is also known as “Zicao” in traditional Chinese medicine, has been proved to have exhibits remarkable anti-tumor properties in several cancers. However, whether ferroptosis participated in the shikonin mediated anti-OS activity still remains to be clarified. Herein, we provide evidence that shikonin possesses the capability to induce the ferroptosis, and elucidate the underlying mechanisms in the treatment of OS. In the present study, it was found that shikonin significantly suppressed OS cells proliferation and blocked the cell cycle progression in vitro. Subsequent results revealed that shikonin could trigger ferroptosis in OS cells by promoting the Fe2+ accumulation, reactive oxygen species and lipid peroxidation formation, malondialdehyde production and mitochondrial damage. Further study showed that the effects of OS cell proliferation and death caused by shikonin could be successfully reversed by ferroptosis inhibitor ferrostatin-1, indicating that ferroptosis participated in the shikonin mediated anti-OS activity. Mechanistically, shikonin physically interacted with Nrf2, a critical regulator of ferroptosis, and influenced Nrf2 stability via inducing ubiquitin degradation, which suppressed the expression of Nrf2 downstream targets xCT and GPX4, and led to stimulating ferroptosis. Collectively, our findings indicated that shikonin induced OS cells ferroptosis through Nrf2/xCT/GPX4 regulatory axis, which might shed light on exploiting shikonin as a promising candidate for the future OS therapy.
Mitigating surface vortex formation in pump sump intakes through anti-vortex devices: a comprehensive CFD study
Authors: Alaboodi Abdulaziz S. , Hussain Zahid , Abbas Saqlain , Rehman Masood ur , Zulfiqar Asim
Published: December 2024

The formation of surface vortices in axial pump sumps presents a significant challenge to pump performance, primarily due to the risk of impeller cavitation. As such, effective mitigation strategies are imperative. In this study, the strength of surface vortices was successfully reduced to a safe operational level by employing two distinct types of anti-vortex devices (AVDs): triangular side fins type (AVDSF) and ring type (AVDR). Computational fluid dynamics (CFD) techniques were employed to reveal a substantial decrease in surface vorticity from 38 s-1 in the absence of AVDs to approximately 8 s-1 with either AVDSFs or AVDRs, underscoring their remarkable efficacy. Further, the helicity, a measure of vortex twisting, was reduced from about 0.4 m2s-2 to below 0.1 m2s-2 with the introduction of either side fins or ring-type AVDs. Detailed analyses of velocity streamlines contours elucidated that the suppression of surface vortices could be attributed to the disruption of vortex swirl motion induced by the implementation of AVDs. These findings provide crucial insights into the mechanisms underlying surface vortex suppression, thus paving the way for enhanced pump performance and reliability in axial pump’s suction sump applications. The implementation of AVDs is expected to prevent cavitation, air ingress, and vortex-induced vibrations, resulting in more reliable and efficient pump operation in industrial settings.
Nernst–Planck–Gaussian finite element modelling of Ca2+ electrodiffusion in amphibian striated muscle transverse tubule–sarcoplasmic reticular triadic junctional domains
Authors: Rodríguez Marco D. , Morris Joshua A. , Bardsley Oliver J. , Matthews Hugh R. , Huang Christopher L.-H.
Published: December 2024

Introduction

Intracellular Ca2+ signalling regulates membrane permeabilities, enzyme activity, and gene transcription amongst other functions. Large transmembrane Ca2+ electrochemical gradients and low diffusibility between cell compartments potentially generate short-lived, localised, high-[Ca2+] microdomains. The highest concentration domains likely form between closely apposed membranes, as at amphibian skeletal muscle transverse tubule–sarcoplasmic reticular (T-SR, triad) junctions.

Materials and methods

Finite element computational analysis characterised the formation and steady state and kinetic properties of the Ca2+ microdomains using established empirical physiological and anatomical values. It progressively incorporated Fick diffusion and Nernst–Planck electrodiffusion gradients, K+, Cl−, and Donnan protein, and calmodulin (CaM)-mediated Ca2+ buffering. It solved for temporal–spatial patterns of free and buffered Ca2+, Gaussian charge differences, and membrane potential changes, following Ca2+ release into the T-SR junction.

Results

Computational runs using established low and high Ca2+ diffusibility (DCa2+) limits both showed that voltages arising from intracytosolic total [Ca2+] gradients and the counterions little affected microdomain formation, although elevated DCa2+ reduced attained [Ca2+] and facilitated its kinetics. Contrastingly, adopting known cytosolic CaM concentrations and CaM-Ca2+ affinities markedly increased steady-state free ([Ca2+]free) and total ([Ca2+]), albeit slowing microdomain formation, all to extents reduced by high DCa2+. However, both low and high DCa2+ yielded predictions of similar, physiologically effective, [Ca2+-CaM]. This Ca2+ trapping by the relatively immobile CaM particularly increased [Ca2+] at the junction centre. [Ca2+]free, [Ca2+-CaM], [Ca2+], and microdomain kinetics all depended on both CaM-Ca2+ affinity and DCa2+. These changes accompanied only small Gaussian (∼6 mV) and surface charge (∼1 mV) effects on tubular transmembrane potential at either DCa2+.

Conclusion

These physical predictions of T-SR Ca2+ microdomain formation and properties are compatible with the microdomain roles in Ca2+ and Ca2+-CaM-mediated signalling but limited the effects on tubular transmembrane potentials. CaM emerges as a potential major regulator of both the kinetics and the extent of microdomain formation. These possible cellular Ca2+ signalling roles are discussed in relation to possible feedback modulation processes sensitive to the μM domain but not nM bulk cytosolic, [Ca2+]free, and [Ca2+-CaM], including ryanodine receptor-mediated SR Ca2+ release; Na+, K+, and Cl− channel-mediated membrane excitation and stabilisation; and Na+/Ca2+ exchange transport.
A motor imagery classification model based on hybrid brain-computer interface and multitask learning of electroencephalographic and electromyographic deep features
Authors: Cao Yingyu , Gao Shaowei , Yu Huixian , Zhao Zhenxi , Zang Dawei , Wang Chun
Published: December 2024

Objective

Extracting deep features from participants’ bioelectric signals and constructing models are key research directions in motor imagery (MI) classification tasks. In this study, we constructed a multimodal multitask hybrid brain-computer interface net (2M-hBCINet) based on deep features of electroencephalogram (EEG) and electromyography (EMG) to effectively accomplish motor imagery classification tasks.

Methods

The model first used a variational autoencoder (VAE) network for unsupervised learning of EEG and EMG signals to extract their deep features, and subsequently applied the channel attention mechanism (CAM) to select these deep features and highlight the advantageous features and minimize the disadvantageous ones. Moreover, in this study, multitask learning (MTL) was applied to train the 2M-hBCINet model, incorporating the primary task that is the MI classification task, and auxiliary tasks including EEG reconstruction task, EMG reconstruction task, and a feature metric learning task, each with distinct loss functions to enhance the performance of each task. Finally, we designed module ablation experiments, multitask learning comparison experiments, multi-frequency band comparison experiments, and muscle fatigue experiments. Using leave-one-out cross-validation(LOOCV), the accuracy and effectiveness of each module of the 2M-hBCINet model were validated using the self-made MI-EEMG dataset and the public datasets WAY-EEG-GAL and ESEMIT.

Results

The results indicated that compared to comparative models, the 2M-hBCINet model demonstrated good performance and achieved the best results across different frequency bands and under muscle fatigue conditions.

Conclusion

The 2M-hBCINet model constructed based on EMG and EEG data innovatively in this study demonstrated excellent performance and strong generalization in the MI classification task. As an excellent end-to-end model, 2M-hBCINet can be generalized to be used in EEG-related fields such as anomaly detection and emotion analysis.
Physiological traits, gene expression responses, and proteomics of rice varieties varying in heat stress tolerance at the flowering stage
Authors: Guo Hui , Tao Wei , Gao Huiyong , Chen Lei , Zhong Xiaoyuan , Tang Maoyan , Gao Guoqing , Liang Tianfeng , Zhang Xiaoli
Published: December 2024

Introduction/Background

Global warming greatly limits the productivity of rice. Rice plants are highly sensitive to heat stress at the flowering stage. The selection of heat-tolerant varieties is considered the most effective approach for ensuring global food security in the coming decades.

Methods

Based on previous screening and QTL localization results, we selected tolerant varieties (Huang Huazhan, HZ) and susceptible varieties (Yang Dao6, YD) of rice and studied their physiological characteristics, gene expression responses, and proteomic differences of their anthers under heat stress. The differentially expressed proteins (DEPs) were validated by real-time PCR.

Results

The activities of the antioxidant enzymes CAT, SOD, POD, and APX were 8.36%, 9.56%, 20.61%, and 25.34% higher in HZ than in YD under heat stress, respectively. Similarly, the content of proline and soluble sugar was 8.32% and 14.47% higher in HZ than in YD, respectively. The content of MDA and H2O2 was 8.11% and 39.5% lower in HZ than in YD, respectively. The ratio of endogenous GA3/ABA in HZ was 10.65, which was significantly higher than that of YD (3.84). In addition, we validated the candidate genes LOC_Os08g07010 and LOC_Os08g07440 that our team located in 2021, and the result showed that the expression of these two heat-tolerant genes in the anthers was significantly higher in HH than in YH. DEPs involved in the response to heat stress were identified by TMT proteomics, five upregulated and three downregulated differential expression proteins in HH. DEPs were verified by RT-qPCR.

Discussion

These results provide new insights into the physiological characteristics, dominant DEPs, and gene expression responses in both rice varieties under heat stress. Our results indicate that the antioxidant and osmoregulatory capacities, the ratio of endogenous GA3 and ABA, these DEPs are mainly involved in the pathways of phenylpropanoid biosynthesis, ubiquitin-mediated proteolysis, carbohydrate metabolism, thiamine metabolism, protein processing in the endoplasmic reticulum, and folding, sorting, and degradation were upregulated to a greater degree in HZ than in YD. Additional studies were performed to clarify the roles of these proteins in response to heat stress.
Satisfying basic psychological needs through a recreational sports programme for people with intellectual disability: human growth and adapted sport in focus
Authors: Crespo-Eguílaz Nerea , Gambra Leyre , Varela Apolinar , Fraguela-Vale Raúl
Published: December 2024

Introduction

Self-determination theory (SDT) highlights the importance of satisfying people’s basic psychological needs (BPN) (autonomy, competence and relatedness) in order to ensure their personal growth and wellbeing. In this regard, sport and physical activity (PA) have been shown to offer significant health benefits, particularly in the case of people with intellectual disability (ID), among whom the benefits are even more noticeable owing to their low levels of PA, sedentary lifestyle, limited opportunities to exercise, and consequently lower quality of life. The aim of this study is to assess the impact of the Más Que Tenis (“More Than Just Tennis”) inclusive recreational sports programme on the satisfaction of BPN among athletes with ID, taking into account factors such as age, gender and type of activity.

Methods

The sample for the study comprised 50 athletes (68% male) with ID (IQ: X = 54.33; sd = 13.43), aged 17–54 years. Data were collected using the Spanish version of the Basic Psychological Needs in Exercise Scale (BPNES), Kaufman Brief Intelligence Test (K-BIT), Satisfaction With Life Scale (SWLS), multidimensional AF5 self-concept scale, Vineland-3 Scale, and MABC-2 observation checklist.

Results

High levels of satisfaction of all three BPN, with autonomy scoring lowest of the three. Gender was found to be a significant predictor, with men scoring higher in all BNP. Participants reported positive perceptions in relation to physical self-concept and satisfaction with life. In terms of adaptive behaviour, deficits were detected in relation to communication and daily living skills, but not in relation to interpersonal skills. With respect to motor behaviour, satisfaction of BPN was found to correlate more with dynamic environments than with static ones. The findings indicate the effectiveness of the Más Que Tenis programme in satisfying the BPN of people with ID. Participants reported improved physical skills, competence, social integration and interpersonal relations, though also insufficient autonomy in relation to choosing what exercises to do.

Conclusion

Satisfaction of BPN through the programme was found to correlate positively with athletes’ satisfaction with life and adaptive development, leading to improved personal and neuropsychological wellbeing.
Using cerebrospinal fluid biomarkers to diagnose Alzheimer’s disease: an Australian perspective
Authors: Eratne Dhamidhu , Collins Steven , Nestor Peter J. , Pond Dimity , Velakoulis Dennis , Yates Mark , Masters Colin L.
Published: December 2024

Cerebrospinal fluid (CSF) biomarkers are currently the only clinically validated biofluid diagnostic test for Alzheimer’s Disease (AD) available in Australia. Testing of CSF biomarkers via lumbar puncture (LP), including quantification of amyloid-β peptide, total tau protein, and phosphorylated tau, can give insight into underlying pathophysiological changes and provide greater certainty in confirming or excluding the presence of Alzheimer’s disease changes compared to standard clinical and radiological assessments. Despite CSF analysis being a safe and cost-effective diagnostic method, the use of CSF biomarkers in the evaluation of potential AD remains limited in Australian clinical practice due to a variety of factors, including regional access challenges, concerns over the perceived invasiveness of LP and a lack of confidence among clinicians in interpreting the results. The advent of disease-modifying therapies as a potential new treatment strategy to reduce the rate of progression in people with AD will drive the demand for early diagnosis of AD. This perspective argues for broader adoption of CSF biomarker testing by providing evidence-based, clinically informed expert guidance on when and why to consider CSF biomarker testing.
Effective green cover and equipment–infrastructure attributes of public green spaces in a Mexican metropolitan area
Authors: Ramos-Palacios Carlos Renato , Banda-Escalante Miriam Edith , Barba-Romo Cecilia Fernanda , Cisneros-Vidales Alicia Anahí , Rodríguez-Herrera Jorge Guillermo
Published: December 2024

In the face of excessive urban growth, urban green spaces face the challenge of efficiently providing ecosystem and environmental services benefits. While public green spaces (PGS) stand out for their different environmental and social benefits, their efficiency depends on the vegetated cover, which can be evaluated in relation to the area, type of polygon, and degree of equipment. In this study, the effective green cover (EGC) assessed from a geographic information system, and the level of equipment–infrastructure were evaluated in different green spaces in the metropolitan area of San Luis Potosí, Mexico. The PGS categories included park with hydrological potential, urban park, linear park, neighborhood park, local garden, residual green space, and sports area. In our results, the urban park and the park with hydrological potential indicated 69.5 and 79.5% of EGC, respectively, and a value of 0.3 of Normalized Difference Vegetation Index (NDVI). Specifically, only in urban park, the land surface temperature (LST) decreased with the increase in the NDVI. The total green coverage of PGS was 6.7 m2 and the EGC was 5.8 m2, which is largely due to the large-sized parks. Furthermore, the provision of parks is insufficient compared to other metropolitan areas on national and international scales. In the spaces with the highest score of equipment, the outstanding elements were urban furniture, children’s playgrounds, and exercise areas, which varied according to the type of green space, indicating a differentiated social use. This study suggests that EGC can be a parameter to assess the green proportion of PGS in cities. Integrating this variable in PGS planning and design can enhance ecosystem services provision in metropolitan areas.
What do policymakers need to know about harassment in the metaverse?
Authors: McIntosh Verity , Allen Catherine
Published: December 2024

As immersive technologies and spatial computing paradigms move into the mainstream, public and political interest in the metaverse is growing. In some respects, the metaverse offers an exciting view of the future, one in which a global community can meaningfully connect regardless of where they are in the world. In contrast, however, early instances of “proto-metaverse” spaces have been plagued by reports of harassment and abuse. Policymakers around the world are now considering the role that governments might play in the regulation and governance of metaverse spaces, seeking to secure protections for citizens, and criminal accountability for offenders in this fast-evolving space. This paper introduces some of the key issues for governments engaging with this topic, including the suitability of existing legislative frameworks, and consideration of a new category of harm that seeks to recognise the distinctive impact of “conduct” abuses in metaverse environments.
Assessment of myocardial function in Retrievers with dilated cardiomyopathy using 2D speckle tracking echocardiography: a pilot study
Authors: Hamabe Lina , Shimada Kazumi , Hirose Miki , Hasegawa Mizuki , Takeuchi Aki , Yoshida Tomohiko , Azakami Daigo , Mandour Ahmed S. , Tanaka Ryou
Published: December 2024

Early diagnosis of canine dilated cardiomyopathy (DCM) is complicated by the presence of a prolonged asymptomatic phase, for which a comprehensive evaluation of myocardial function is essential. This pilot study was conducted to evaluate the myocardial function in dogs with DCM using two-dimensional speckle tracking echocardiography (2D-STE). Nine client-owned Retrievers with DCM and twelve client-owned clinically normal Retrievers were comparatively evaluated using standard echocardiography and 2D-STE. Dogs with DCM were characterized by significant dilation of the left ventricle (LV), thinning of the LV wall, and myocardial hypokinesis when compared to clinically normal dogs. The global strain analysis showed a significant reduction of strain in both radial and circumferential directions, and the regional strain analysis revealed a greater degree of myocardial dysfunction at the LV free wall in the circumferential direction in dogs with DCM. The regional strain analysis also demonstrated a difference in the pattern of contraction between dogs with DCM and clinically normal dogs. The results of this study illustrate the ability of 2D-STE to evaluate both global and regional myocardial function in dogs with DCM and show differences between dogs with DCM and clinically normal dogs.
Calcium Hydroxide–Phosphate-Modified Fly Ash Enhances the Adsorption and Stabilization of Soil Lead and Cadmium
Authors: Cui Hongbiao , Xu Zhi , Guo Tengfei , Hu Shaojun , Xia Ruizhi , Zhang Shiwen , Wang Yin , Zhou Jun
Published: December 2024

Fly ash (FA) is characterized by its porous structure and richness in silicon and aluminum oxides; thus, it can be used as an adsorbent for heavy metals. In order to enhance the absorption efficiency and stabilization effect, we prepared a new fly ash (FAKCa) using calcium hydroxide (Ca(OH)2) and phosphate (KH2PO4) through a simple one-step low-temperature alkali dissolution method and investigated its adsorption performance for lead and cadmium in water solutions and the stabilization effects of lead and cadmium in soils under flooding condition. Results showed that the Langmuir model best fit the adsorption behavior of lead and cadmium, and the maximal adsorption capabilities of lead (128 mg/g) and cadmium (39.1 mg/g) for FAKCa were increased by 236% and 14.5% compared with the unmodified FA, respectively. The adsorption of lead and cadmium by FAKCa was better fitted to the second-order kinetic model. The enhancement of adsorption capacities for lead and cadmium may be partly due to the specific surface area of FAKCa, which was increased by 94.0% compared to unmodified FA. FTIR, XRD, and XPS analysis showed that the Si-O and Al-O functional groups, carbonate, and hydroxide precipitation were facilitated by the adsorption of lead and cadmium. Thus, ion exchange, surface complexation, and formation of metal hydroxide and carbonate precipitation were the main adsorption mechanisms for lead and cadmium by FAKCa. In addition, the application of 0.1–0.6% FAKCa increased soil pH by 0.19–0.67 units and decreased the CaCl2-extractable lead by 12.3–86.5% compared to FA. Meanwhile, FAKCa was more effective in transforming lead and cadmium from exchangeable to stable fractions. This study shows that calcium hydroxide–phosphate-modified fly ash could effectively increase the adsorption and stabilization of lead and cadmium and, thus, has great potential for large-scale applications in contaminated soil.
FoxO1 Mediated by H3K27me3 Inhibits Porcine Follicular Development by Regulating the Transcription of CYP1A1
Authors: Zhou Zhi , Lv Yuanyuan , Li Liying , Yuan Xiaolong , Zhou Xiaofeng , Li Jiaqi
Published: December 2024

It is well known that the function of granulosa cells (GCs) is closely related to follicular development, and FoxO1 and histone methylation have been implicated in follicular development. However, the specific mechanisms by which FoxO1 and histone methylation regulate follicular development are still largely unknown. To explore the specific mechanism of FoxO1 in regulating follicular development, in this study, we showed that the expression of FoxO1 in immature ovaries and small follicles was significantly higher than in mature ovaries and large follicles of sows, respectively. FoxO1 was found to inhibit the secretion of testosterone and proliferation of porcine GCs and promote the secretion of progesterone and apoptosis of porcine GCs. Furthermore, H3K27me3, as a transcriptional inhibitor, can inhibit the transcription of FoxO1. FoxO1 could promote the transcription of CYP1A1, and CYP1A1 was found to inhibit the proliferation and facilitate the ferroptosis of porcine GCs. Collectively, our results revealed that the H3K27me3-FoxO1-CYP1A1 pathway might participate in follicular development, and these findings could provide potential targets for improving follicular development in sows.
Simultaneous Effects of Single-Nucleotide Polymorphisms on the Estimated Breeding Value of Milk, Fat, and Protein Yield of Holstein Friesian Cows in Hungary
Authors: Bognár László , Kőrösi Zsolt Jenő , Bene Szabolcs Albin , Szabó Ferenc , Anton István , Zsolnai Attila
Published: December 2024

This study aimed to find SNPs that have an effect on the estimated breeding values (EBVs) of milk (MY), fat (FY), and protein yield (PY) of Holstein Friesian cows in Hungary. Holstein Friesian cows (n = 2963) were genotyped on a Eurogenomics (EuroG_MDv4) chip. The EBVs for MY, FY, and PY were obtained from the Association of Hungarian Holstein Breeders (AHHB). The loci associated with the EBVs were identified via three approaches: the calculation of genetic distance of the SNPs (Fst_marker), linear regression, and haplotype association tests. Nine SNPs were significantly associated with MY, FY, and PY located on BTA 2, 5, 28, and X. Among the nine SNPs identified, BTB-00219372 on BTA 5 had a positive β coefficient for MY and a negative β coefficient for FY and PY. In addition, BovineHD3000027615 on BTA X had a positive β coefficient for both MY and PY, as well as a negative β coefficient for FY. The identified SNPs were located near several genes that remain unstudied in cattle, which are potential targets for closer scrutiny in relation to milk properties. The markers associated with two or three EBVs could be used in selection with high efficiency to accelerate genetic development and help AHHB experts achieve their breeding. Most marker effects point in the same direction on EBVs; however, we found that BTB-00219372 and BovineHD3000027615 could be used with caution to increase one EBV while decreasing the other EBV or EBVs.
Estimation of Sow Backfat Thickness Based on Machine Vision
Authors: Jian Yue , Pu Shihua , Zhu Jiaming , Zhang Jianlong , Xing Wenwen
Published: December 2024

Controlling the backfat thickness of sows within an appropriate range during different production stages helps to increase the number of pigs weaned per sow per year and ultimately enhances the economic benefit to the pig farm. To obtain the backfat thickness of sows automatically, a backfat thickness estimation method based on machine vision is proposed. First, the backfat thickness values and 3D images of the buttocks of 154 Landrace–Yorkshire crossbred sows were obtained using a veterinary ultrasound backfat meter and Azure Kinect DK camera. After preprocessing the 3D images utilizing Python 3.9.16 software, 10 external morphological parameters reflecting the area, width, height, and contour radius of the sow’s buttocks were extracted. The relationships between backfat thickness and external morphological parameters were analyzed in a randomly selected group of 100 sows. A significant positive correlation was observed between backfat thickness and buttock morphological parameters, with the Pearson coefficient for the fitted ellipse area achieving values up to 0.90. A backfat thickness estimation model was developed based on selected buttock feature parameters. The model’s generalization performance was evaluated using 54 additional sows that were not involved in the model development. The coefficient of determination (R2) between the estimated and actual backfat thicknesses was 0.8923, with a mean absolute error (MAE) of 1.23 mm and a mean absolute percentage error (MAPE) of 5.73%. These metrics indicate that the model can meet production requirements, and the proposed technique offers improved estimation accuracy compared to existing methods. Ultimately, a backfat thickness automatic estimation system was developed using LabVIEW 2023 Q1 (64-bit) software. This research helps to address the cumbersome process of measuring sow backfat thickness and promotes the automation of sow farms.
Factors Influencing Contrast Enhancement in Abdominal Computed Tomography Angiography in the Dog: A Systematic Review
Authors: Perfetti Simone , Guglielmini Carlo , Linta Nikolina , Diana Alessia
Published: December 2024

Multidetector-row computed tomographic angiography (angio-CT) aims to achieve optimal opacification of the vascular compartment of interest. The distribution and quality of vascular opacification are influenced by patient-related factors, contrast medium (CM)-related factors, and scanner-related factors. This systematic review evaluates these factors and their effects on contrast enhancement. A comprehensive literature search was made in February 2024 across four online bibliographic databases (Web of Science, PubMed, Scopus, and CAB Abstract) in adherence with the PRISMA 2020 guidelines. After screening the 5990 unique published articles initially identified, 20 full-text original studies met the inclusion criteria for the final review. The amount of abdominal adipose tissue was found to significantly affect enhancement, which suggests the possibility of reducing the CM dose to minimize adverse effects or toxicity. The injection rate of the CM, rather than the injection duration, was identified as the most critical factor, with important clinical implications. For scanners with slower acquisition speeds or longer scan durations, maintaining a fixed CM injection duration may optimize vascular phase acquisition. In contrast, faster scanners benefit from bolus tracking, which allows for improved differentiation between vascular phases. Additionally, administering a saline flush post-CM injection enhances arterial opacification while reducing the necessary CM dose. This systematic review highlights essential factors influencing contrast enhancement in angio-CT for dogs and provides a foundation for future research aimed at optimizing imaging protocols in veterinary medicine.
Stable Solutions of a Class of Degenerate Elliptic Equations
Authors: Lang Yin , Liu Hairong
Published: December 2024

This paper deals with the second-order semi-linear degenerate elliptic equation yuyy+buy+Δxu+|u|α−1u=0,(x,y)∈Rn×(0,∞), where n≥1,α>1. We establish a Liouville theorem of stable solution of the degenerate equation mentioned above by using the energy method. The classification results for stable solutions belonging to C2 can be thought of as an analogue of the recent results of Farina for the Lane–Emden equation.
Learning the Ageing Behaviour of Lithium-Ion Batteries Using Electric Vehicle Fleet Analysis
Authors: Lehmann Thomas , Berendes Erik , Kratzing Richard , Sethia Gautam
Published: December 2024

This article presents the results of the Febal project, where the aim was to parametrize a stress-factor-based ageing model for Lithium-ion batteries using operation data of an electric fleet. Contrary to state-of-the-art methods, this approach does not rely on laboratory ageing tests only. Instead, a novel physics-informed learning procedure is used to combine the accuracy and flexibility of data-driven approaches with the extrapolation properties of physical models. The ageing model is parameterized in a two-stage process. In order to cover data ranges not present in operation, a laboratory ageing test campaign is used as a baseline. In the second stage, transfer learning is used to adjust a subset of the model parameters to fit data of different cells. This approach is not only applied to laboratory measurements but also validated by a series of capacity checkup tests performed with a fleet of electric vehicles. Results show the improved state-of-health (SOH) prediction of the proposed model parameterization method.
Neural Precursor Cell-Expressed Developmentally Downregulated Protein 4 (NEDD4)-Mediated Ubiquitination of Glutathione Peroxidase 4 (GPX4): A Key Pathway in High-Glucose-Induced Ferroptosis in Corpus Cavernosum Smooth Muscle Cells
Authors: Xu Wenchao , Hu Peng , Wang Jiaxin , Jiang Hongyang , Wang Tao , Liu Jihong , Li Hao
Published: December 2024

Pharmacological treatment of diabetes mellitus-induced erectile dysfunction (DMED) has become increasingly challenging due to the limited efficacy of phosphodiesterase type 5 inhibitors (PDE5i). As the global prevalence of DM continues, there is a critical need for novel therapeutic strategies to address DMED. In our previous studies, we found that Glutathione peroxidase 4 (GPX4), a ferroptosis inhibitor, can ameliorate DMED in diabetic rats. However, the specific role of GPX4 in corpus cavernosum smooth muscle cells (CCSMCs) and its regulatory mechanisms remain unclear. In this study, we established primary cultures of CCSMCs and systematically analyzed the role of GPX4 under high-glucose conditions. To further elucidate the upstream regulatory pathways of GPX4, we employed immunoprecipitation coupled with mass spectrometry (IP-MS) to identify potential interacting proteins. Additionally, co-immunoprecipitation (Co-IP) and cycloheximide (CHX) chase assays were conducted to explore the regulatory dynamics and post-translational stability of GPX4. Under high-glucose conditions, the expression of GPX4 in CCSMCs is significantly downregulated, leading to an increase in intracellular oxidative stress and heightened levels of ferroptosis, accompanied by dysfunction in smooth muscle cell relaxation. Furthermore, the CHX chase assay revealed that high glucose accelerates GPX4 protein degradation via the ubiquitin–proteasome pathway. Subsequent IP-MS identified NEDD4, an E3 ubiquitin ligase, as a potential interacting partner of GPX4. Further validation demonstrated that NEDD4 modulates the ubiquitination process of GPX4, thereby influencing its stability and expression. In conclusion, we identified NEDD4 as a key regulator of GPX4 stability through ubiquitin-mediated proteasomal degradation. These findings suggest potential therapeutic strategies targeting the NEDD4-GPX4 axis to alleviate DMED pathology.
Flexible Electrochemical Biosensor Using Nanostructure-Modified Polymer Electrode for Detection of Viral Nucleic Acids
Authors: Han Jiyu , Lee Yejin , Lee Jin-Ho , Yoon Jinho
Published: December 2024

In the biosensor field, the accurate detection of contagious disease has become one of the most important research topics in the post-pandemic period. However, conventional contagious viral biosensors normally require chemical modifications to introduce the probe molecules to nucleic acids such as a redox indicator, fluorescent dye, or quencher for biosensing. To avoid this complex chemical modification, in this research, mismatched DNA with an intercalated metal ion complex (MIMIC) is employed as the probe sequence. In addition, the MIMIC is fabricated on a lithography-assisted nanostructure-modified flexible polymer electrode. On this flexible electrode, as a proof-of-concept study, a human papillomavirus (HPV-16 and -18) was detected by the MIMIC with a high accuracy. The developed biosensor exhibits an ultrasensitive ability to detect HPV in viral DNA without target amplification and chemical modifications in a simple preparation manner. Moreover, it retains its nanostructures and high conductivity after bending. In conclusion, the use of the proposed biosensor suggests a novel approach to developing an ultrasensitive and flexible biosensor for the detection of important biomarkers in a simple manner that can be applied in point-of-care testing.
Exploring Simultaneous Effects of Delay Factors in Precast Concrete Installation
Authors: Jang Junyoung , Jeong Eunbeen , Cho Jongwoo , Kim Tae Wan
Published: December 2024

Delays in the installation process of precast concrete (PC) components significantly impact the project execution. However, traditional scheduling and risk assessment methods fail to consider this process complexity and uncertainty adequately. With a systematic approach, this study analyzed complex delay mechanisms in the PC installation of three component types (columns, beams, and slabs) using 1881 observations across five work steps. Specifically, this study used k-means clustering to divide the observations into groups with certain characteristics. These groups were assessed quantitatively using the delay intensity metric. Based on the assessment, this study revealed six severe delay paths for different component types, which may combine to generate severe combinations of delay factors, considering factors such as the component size, wind conditions, worker availability, and installation location. This research contributed to PC construction management by presenting a systematic analysis of delay factors and by proposing specific severe delay paths during PC installation, offering project managers a basis for schedule optimization and risk management.
Evolving Treatment Landscape of Frontline Therapy for Metastatic Urothelial Carcinoma: Current Insights and Future Perspectives
Authors: Kwon Whi-An , Lee Min-Kyung
Published: December 2024

Cisplatin-based chemotherapy has long been the standard first-line (1L) treatment for metastatic urothelial carcinoma (mUC). However, up to 50% of patients with mUC may be ineligible for cisplatin owing to comorbidities, necessitating alternative primary treatment options. Immune checkpoint inhibitors (ICIs) have emerged as a vital alternative for those unable to receive cisplatin. Nevertheless, the prognosis of advanced UC remains dire and challenges persist in optimizing 1L therapy. Recent medical advancements have redirected attention towards innovative drug combinations for the primary treatment of mUC. The combination of enfortumab vedotin (EV) and pembrolizumab has shown significantly improved overall and progression-free survival rates compared to those with chemotherapy alone. This combination can be used as a 1L treatment for patients with mUC who are cisplatin-ineligible or require alternatives to standard chemotherapy. While platinum-based chemotherapy continues to be essential for many patients, the approval of EV and pembrolizumab as 1L treatments for cisplatin-ineligible patients signifies a major breakthrough in primary cancer care. These therapies offer enhanced outcomes in terms of survival and response rates and highlight the increasing relevance of ICI-containing regimens in frontline cancer care. This review provides an exhaustive overview of the current frontline treatment landscape of mUC and explores new therapeutic strategies, with the aim of facilitating clinical decision-making and guiding therapeutic strategies in patients with mUC.
Predictive Factors of Antibody–Drug Conjugate Treatment in Metastatic Breast Cancer: A Narrative Review
Authors: Gadaleta-Caldarola Gennaro , Lanotte Laura , Santoro Anna Natalizia , Pinto Antonello , Gadaleta-Caldarola Arianna , Giacomelli Luca , Fedele Palma
Published: December 2024

Antibody–drug conjugates (ADCs) have revolutionized the treatment landscape for metastatic breast cancer, offering targeted delivery of cytotoxic agents with improved efficacy and tolerability compared to conventional chemotherapy. This narrative review explores key predictive factors influencing the efficacy of ADCs, focusing on HER2-targeted therapies, such as trastuzumab emtansine and trastuzumab deruxtecan, as well as sacituzumab govitecan for triple-negative breast cancer. HER2 expression, TROP-2 levels, hormone receptor status, and the tumor microenvironment emerge as critical biomarkers for patient selection and therapeutic outcomes. Additionally, we discuss resistance mechanisms, such as antigen loss, impaired drug internalization, and the role of circulating tumor DNA in predicting ADC response. Finally, future perspectives on the sequential use of ADCs and potential combination therapies are highlighted, along with emerging agents targeting alternative antigens like HER3 and LIV-1. Overall, identifying predictive biomarkers and overcoming resistance mechanisms are essential for optimizing the use of ADCs in metastatic breast cancer, thereby improving patient outcomes.