bluuebunny/crossref_metadata_2025 huggingface dataset:
An Automatic Recognition Method for Fish Species and Length Using an Underwater Stereo Vision System
Authors: Deng Yuxuan , Tan Hequn , Tong Minghang , Zhou Dianzhuo , Li Yuxiang , Zhu Ming
Published: November 2022

Developing new methods to detect biomass information on freshwater fish in farm conditions enables the creation of decision bases for precision feeding. In this study, an approach based on Keypoints R-CNN is presented to identify species and measure length automatically using an underwater stereo vision system. To enhance the model’s robustness, stochastic enhancement is performed on image datasets. For further promotion of the features extraction capability of the backbone network, an attention module is integrated into the ResNeXt50 network. Concurrently, the feature pyramid network (FPN) is replaced by an improved path aggregation network (I-PANet) to achieve a greater fusion of effective feature maps. Compared to the original model, the mAP of the improved one in object and key point detection tasks increases by 4.55% and 2.38%, respectively, with a small increase in the number of model parameters. In addition, a new algorithm is introduced for matching the detection results of neural networks. On the foundation of the above contents, coordinates of head and tail points in stereo images as well as fish species can be obtained rapidly and accurately. A 3D reconstruction of the fish head and tail points is performed utilizing the calibration parameters and projection matrix of the stereo camera. The estimated length of the fish is acquired by calculating the Euclidean distance between two points. Finally, the precision of the proposed approach proved to be acceptable for five kinds of common freshwater fish. The accuracy of species identification exceeds 94%, and the relative errors of length measurement are less than 10%. In summary, this method can be utilized to help aquaculture farmers efficiently collect real-time information about fish length.
Causes of Mortality and Loss of Lumpfish Cyclopterus lumpus
Authors: Reynolds Patrick , Imsland Albert Kjartan Dagbjartarson , Boissonnot Lauris
Published: November 2022

Data from research and commercial use of lumpfish were collected from the research facilities of Gifas (Inndyr, Northern Norway, 67.0° N, 14.0° E). The data were sourced from 12 main lumpfish groups subdivided into 66 subgroups (N = 160,729) delivered to Gifas between 2013 and 2020 and used in cleaner-fish research in (a) land-based facilities, (b) small-scale, or (c) large-scale sea pens. The data were standardised and organised into three main headings. Firstly, background information included transfer time, point of origin, mean starting weight and population size on arrival. Other information included to which site the lumpfish were transferred, volume of cage/tank, whether in the presence of salmon or not, stocking density, days at each site and water quality parameters. Causes of mortality were recorded, when possible, for each group, along with calculated mortality rates, patterns, and analytical information, along with imaging where available. Results show that causes of mortality varied within and between research sites. For lumpfish in hatcheries as well as for those deployed at small-scale sea pens, the primary cause of mortality was identified as pathogenic, while for lumpfish deployed at large-scale sea pens, transporting, grading and mechanical delousing were the primary causes of mortality. The results indicate that more research is required to clarify best practices both in commercial hatcheries and salmon cages and further understanding on lumpfish biological requirements and stress physiology is necessary to develop better methods that safeguard lumpfish welfare and meet their needs.
Transcriptome Analysis of Chinese Cabbage Provides Insights into the Basis of Understanding the Lignin Affected by Low Temperature
Authors: Dai Yun , Wang Shaoxing , Huang Wenyue , Li Ze , Zhang Shifan , Zhang Hui , Li Guoliang , Fang Zhiyuan , Sun Rifei , Li Fei , Zhang Shujiang
Published: November 2022

Chinese cabbage, which is a cold season crop, can still be damaged at an overly low temperature. It is crucial to study the mechanism of the resistance to low temperature of Chinese cabbage. In this study, the Chinese cabbage ‘XBJ’ was used as the material, and nine different low temperatures and control samples were treated. Using RNA-seq and lignin content determination, we analyzed 27 samples, and the stained sections of them were observed. A total of 8845 genes were screened for the WGCNA analysis, yielding 17 modules. The GO and KEGG analyses of the modules was highly associated with a low-temperature treatment. The pathways such as ‘starch and sucrose metabolism’ and ‘plant hormone signal transduction’ were enriched in modules related to low temperature. Interestingly, L-15DAT-associated MEcoral2 was found to have 14 genes related to the ‘lignin biosynthetic process’ in the GO annotation. The combination of the determination of the lignin content and the treatment of the stained sections showed that the lignin content of the low-temperatures samples were indeed higher than that of the control. We further explored the expression changes of the lignin synthesis pathway and various genes and found that low temperature affects the expression changes of most genes in the lignin synthesis pathway, leading to the speculation that the lignin changes at low temperature are a defense mechanism against low temperatures. The 29 BrCOMT gene sequence derived from the RNA-seq was non-conserved, and eight BrCOMT genes were differentially expressed. This study provides a new insight into how lignin is affected by low temperature.
Single Nucleotide Polymorphism and mRNA Expression of LTF in Oral Squamous Cell Carcinoma
Authors: Gołąbek Karolina , Rączka Grzegorz , Gaździcka Jadwiga , Miśkiewicz-Orczyk Katarzyna , Zięba Natalia , Krakowczyk Łukasz , Hudy Dorota , Asman Marek , Misiołek Maciej , Strzelczyk Joanna Katarzyna
Published: November 2022

Oral squamous cell carcinoma (OSCC) is one of the most prevalent types of cancers worldwide. LTF arrests the G1 to S phase transition of the cell cycle. This study is the first that has aimed to determine the possible association between the LTF polymorphisms (rs2073495, rs1126478, rs34827868, rs1042073, rs4637321, rs2239692 and rs10865941), the mRNA LTF expression, the risk of OSCC and the influence on the TNM staging and histological grading. This study was composed of 176 Polish patients, including 88 subjects diagnosed with OSCC and 88 healthy individuals. QuantStudio Design and Analysis Software v1.5.1 was used for the single nucleotide polymorphism (SNP) analysis and mRNA LTF expression. The G/G genotype of rs2073495 and the G/G genotype of rs4637321 were linked, with an increased risk of OSCC. There were no significant influences between the TNM staging and the histological grading and the LTF genotype. We found no statistically significant dissimilarities in the expression level of LTF genes in the tumour and margin specimens. No association was found between the gene expression levels, the other parameters or LTF polymorphisms in the tumour and margin samples. In conclusion, rs2073495 and rs4637321 polymorphisms may affect the risk of OSCC. These results should be validated on larger and different cohorts to better comprehend the role of the LTF gene in OSCC.
Implementation of Public Funded Genome Sequencing in Evaluation of Fetal Structural Anomalies
Authors: So Po Lam , Hui Annie Shuk Yi , Ma Teresa Wei Ling , Shu Wendy , Hui Amelia Pui Wah , Kong Choi Wah , Lo Tsz Kin , Kan Amanda Nim Chi , Kan Elaine Yee Ling , Chong Shuk Ching , Chung Brian Hon Yin , Luk Ho Ming , Choy Kwong Wai , Kan Anita Sik Yau , Leung Wing Cheong
Published: November 2022

With the advancements in prenatal diagnostics, genome sequencing is now incorporated into clinical use to maximize the diagnostic yield following uninformative conventional tests (karyotype and chromosomal microarray analysis). Hong Kong started publicly funded prenatal genomic sequencing as a sequential test in the investigation of fetal structural anomalies in April 2021. The objective of the study was to evaluate the clinical performance and usefulness of this new service over one year. We established a web-based multidisciplinary team to facilitate case selection among the expert members. We retrospectively analyzed the fetal phenotypes, test results, turnaround time and clinical impact in the first 15 whole exome sequencing and 14 whole genome sequencing. Overall, the molecular diagnostic rate was 37.9% (11/29). De novo autosomal dominant disorders accounted for 72.7% (8/11), inherited autosomal recessive disorders for 18.2% (2/11), and inherited X-linked disorders for 9.1% (1/11). The median turnaround time for ongoing pregnancy was 19.5 days (range, 13–31 days). Our study showed an overall clinical impact of 55.2% (16/29), which influenced reproductive decision-making in four cases, guided perinatal management in two cases and helped future family planning in ten cases. In conclusion, our findings support the important role of genome sequencing services in the prenatal diagnosis of fetal structural anomalies in a population setting. It is important to adopt a multidisciplinary team approach to support the comprehensive genetic service.
Exploratory Analysis of the Sasang Constitution by Combining Network Analysis and Information Entropy
Authors: Lee Won-Yung , Kim Sang Hyuk , Lee Siwoo , Kim Young Woo , Kim Ji-Hwan
Published: November 2022

Sasang constitutional medicine is a unique concept in Korean medicine that can provide valuable insights into personalized healthcare and disease treatment. In this study, we combined network analysis and information entropy to systematically investigate the related information of Sasang constitutional (SC) types. A feature network was constructed using SC type and clinical information. The SC type-associated features and feature classes were identified using statistical analysis and entropy ranking. The patient network was constructed based on SC-type-associated features. We found that the feature network was closely connected within the features of the same classes and between several feature class pairs, including the symptom class. Most of the separation values between the feature classes, including the symptom class, were negative. In addition, we found 42 clinical features related to the SC type, and two important classes -personality and cold/heat- that increase the entropy ranking of the SC type. In the patient network, we found sparsely connected modules between SC types and a positive separation value between the Taeeumin–Soeumin and Taeeumin–Soyangin pairs. Our data-driven approach provides a deeper understanding of modernized forms of SC types and suggests that SC type is a practically useful concept for stratified healthcare and personalized medicine.
A Case of Oral Health Management for a Patient with Extensive Ulceration of the Oral Mucosa Due to Herpes Zoster
Authors: Matsuda Yuhei , Mizuno Ruriko , Miyajima Saki , Arakawa Shinichi , Kabasawa Yuji
Published: November 2022

Oral ulcers caused by herpes zoster virus infection are commonly encountered in daily clinical practice. However, in rare cases, sepsis or viremia can occur with serious outcomes; hence, these must be managed effectively. Here, we report a case of a patient with extensive ulceration caused by varicella zoster virus infection. Antiviral treatment was started early, and oral health management was started simultaneously, with oral hygiene instructions for pain control. As a result, the patient was able to resume oral food intake and was discharged from the hospital within a week. This case suggested that oral health management comprising supportive care, with the assistance of dentists and dental hygienists, as well as antiviral therapy, are important in the treatment of oral ulcers associated with herpes zoster virus infection.
Image and Speech Recognition Technology in the Development of an Elderly Care Robot: Practical Issues Review and Improvement Strategies
Authors: Fahn Chin-Shyurng , Chen Szu-Chieh , Wu Po-Yuan , Chu Tsung-Lan , Li Cheng-Hung , Hsu Deng-Quan , Wang Hsiu-Hung , Tsai Hsiu-Min
Published: November 2022

As the world’s population is aging and there is a shortage of sufficient caring manpower, the development of intelligent care robots is a feasible solution. At present, plenty of care robots have been developed, but humanized care robots that can suitably respond to the individual behaviors of elderly people, such as pose, expression, gaze, and speech are generally lacking. To achieve the interaction, the main objectives of this study are: (1) conducting a literature review and analyzing the status quo on the following four core tasks of image and speech recognition technology: human pose recognition, human facial expression recognition, eye gazing recognition, and Chinese speech recognition; (2) proposing improvement strategies for these tasks based on the results of the literature review. The results of the study on these improvement strategies will provide the basis for using human facial expression robots in elderly care.
Neuroimaging in Tick Paralysis: Looking Outside the Box
Authors: Sarwar Zereen , Osborne Aaron Fletcher , Shah Chetan , Rathore Mobeen H.
Published: November 2022

Tick paralysis is a rare but potentially deadly form of muscle paralysis caused by a neurotoxin transmitted through the saliva of gravid, engorged female ticks of various species. Often, there is an initial misdiagnosis or delay in diagnosis due to the rarity of the diagnosis, the obscure location of the tick, and the disease’s clinical similarity to Guillain–Barre syndrome. We report the case of a 4-year-old girl with tick paralysis in whom the tick was not found until 2 days after hospital admission. Upon the review of the imaging, it was discovered that the tick was visible on the MRI of the brain that had been reported as normal.
Type II Heterojunction Formed between {010} or {012} Facets Dominated Bismuth Vanadium Oxide and Carbon Nitride to Enhance the Photocatalytic Degradation of Tetracycline
Authors: Zhang Xiaojing , Xie Xianglun , Li Jianan , Han Dongfang , Ma Yingming , Fan Yingying , Han Dongxue , Niu Li
Published: November 2022

Both type II and Z schemes can explain the charge transfer behavior of the heterojunction structure well, but the type of heterojunction structure formed between bismuth vanadium oxide and carbon nitride still has not been clarified. Herein, we rationally prepared bismuth vanadium oxide with {010} and {012} facets predominantly and carbon nitride as a decoration to construct a core-shell structure with bismuth vanadium oxide wrapped in carbon nitride to ensure the same photocatalytic reaction interface. Through energy band establishment and radical species investigation, both {010} and {012} facets dominated bismuth vanadium oxide/carbon nitride composites exhibit the type II heterojunction structures rather than the Z-scheme heterojunctions. Furthermore, to investigate the effect of type II heterojunction, the photocatalytic tetracycline degradations were performed, finding that {010} facets dominated bismuth vanadium oxide/carbon nitride composite demonstrated the higher degradation efficiency than that of {012} facets, due to the higher conduction band energy. Additionally, through the free radical trapping experiments and intermediate detection of degradation products, the superoxide radical was proven to be the main active radical to decompose the tetracycline molecules. Therein, the tetracycline molecules were degraded to water and carbon dioxide by dihydroxylation-demethylation-ring opening reactions. This work investigates the effect of crystal planes on heterojunction types through two different exposed crystal planes of bismuth vanadate oxide, which can provide some basic research and theoretical support for the progressive and controlled synthesis of photocatalysts with heterojunction structures.
Costs and Factors Associated with Hospitalizations Due to Severe Influenza in Catalonia (2017–2020)
Authors: Soler-Font Mercè , Aznar-Lou Ignacio , Basile Luca , Soldevila Núria , Godoy Pere , Martínez Ana , Serrano-Blanco Antoni , Domínguez Angela ,   The Surveillance of Hospitalized Cases of Severe Influenza in Catalonia Working Group
Published: November 2022

This study aimed to estimate the cost and factors associated with severe hospitalized patients due to influenza in unvaccinated and vaccinated cases. The study had a cross-sectional design and included three influenza seasons in 16 sentinel hospitals in Catalonia, Spain. Data were collected from a surveillance system of influenza and other acute respiratory infections. Generalized linear models (GLM) were used to analyze mean costs stratified by comorbidities and pregnancy. Multivariate logistic models were used to analyze bacterial coinfection, multi-organ failure, acute respiratory distress syndrome, death and ICU admission by season and by vaccination status. Costs of ICU, hospitalization and total mean costs were analyzed using GLM, by season and by vaccination status. All models were adjusted for age and sex. A total of 2742 hospitalized cases were included in the analyses. Cases were mostly aged ≥ 60 years (70.17%), with recommended vaccination (86.14%) and unvaccinated (68.05%). The ICU admission level was statistically significant higher in unvaccinated compared to vaccinated cases. Costs of cases with more than or equal to two comorbidities (Diff = EUR − 1881.32), diabetes (Diff = EUR − 1953.21), chronic kidney disease (Diff = EUR − 2260.88), chronic cardiovascular disease (Diff = EUR − 1964.86), chronic liver disease (Diff = EUR − 3595.60), hospitalization (EUR 9419.42 vs. EUR 9055.45), and total mean costs (EUR 11,540.04 vs. 10,221.34) were statistically significant higher in unvaccinated compared to vaccinated patients. The influenza vaccine reduces the costs of hospitalization. There is a need to focus strategies in recommended vaccination groups.
Exposure to House Dust Mite Allergen and Endotoxin in Early Life and Sensitization and Allergic Rhinitis: The JECS
Authors: Kojima Reiji , Shinohara Ryoji , Kushima Megumi , Horiuchi Sayaka , Otawa Sanae , Miyake Kunio , Yokomichi Hiroshi , Akiyama Yuka , Ooka Tadao , Yamagata Zentaro ,   the Japan Environment and Children’s Study Group
Published: November 2022

The association between endotoxin and allergic rhinitis (AR) is not conclusive. The aim of this study was to determine the association between endotoxin and house dust mite (HDM) allergens in dust, and HDM sensitization and AR among Japanese infants. This study included 4188 participants in the Sub-Cohort Study of the Japan Environment and Children’s Study. Dust was collected from children’s mattresses at age 18 months and endotoxin and HDM allergen levels were measured. A logistic regression model was used to analyze the association between endotoxin or HDM allergen and the sensitization to HDM (specific-IgE) at age 2 and AR at age 3. The median (interquartile range) endotoxin level was 375.1(186.9–826.5) EU/m2 and the Der 1 (Der p 1 + Der f 1) level was 51.2 (14.8–168.6) ng/m2. There were significant positive associations between endotoxin and HDM sensitization (Der f 1, adjusted odds ratio [aOR] quartile [Q] 4 vs. Q1, 1.44, 95% CI, 1.04–2.00; Der p 1, aOR Q4 vs. Q1, 1.56, 95% CI, 1.12–2.16). There were also significant positive associations between Der 1 exposure and HDM sensitization (Der f 1, aOR Q3 vs. Q1, 1.75, 95% CI, 1.26–2.44; aOR Q4 vs. Q1, 2.98, 95% CI, 2.15–4.13; Der p 1, aOR Q3 vs. Q1, 1.91, 95% CI, 1.37–2.66; aOR Q4 vs. Q1, 2.91, 95% CI, 2.09–4.05). There were, however, no associations between endotoxin or Der 1 and AR. In the population residing mostly in non-farming settings, both endotoxin and HDM allergens in dust were associated with an increased risk of HDM allergen sensitization, but not with AR.
Preschool Children’s Physical Activity and Community Environment: A Cross-Sectional Study of Two Cities in China
Authors: Wang Yu , He Gang , Ma Kaiyue , Li Dongsheng , Wang Chao
Published: November 2022

Research on the relationship between preschool children’s physical activity (PA) and community environment is limited and inconclusive, yet understanding this relationship is important to acquire sufficient information to guide the development of intervention programs. This study aims to objectively measure preschool children’s PA and examine associations between PA and the community environments. A total of 471 preschool children aged 3–6 years old were recruited from eight kindergartens in Beijing and Zhengzhou. PA was measured by accelerometers. Light PA (LPA), moderate PA (MPA), and vigorous PA (VPA) were computed on the basis of cutoff points developed for preschool children. Moderate-to-vigorous PA (MVPA), and total PA (TPA) were obtained by calculation. Children’s active transportation modes were indicated by the frequency of active trips (FAT) reported by parents. The community environment was collected by parental scales. Multivariate linear regression was used to analyze the associations between PA and the community environment. In total, 304 preschool children (mean age 5.07 ± 0.94 years, 50.66% boys) were included in the final analysis. Children spent an average of 77.58 ± 18.78 min/day in MVPA and 173.26 ± 30.38 min/day in TPA. Linear regression showed that ‘parental perception of appropriate walking distance’ was associated with nearly half of the indicators of the children’s PA. ‘Public activity facilities near the community’ was associated with FAT for overall children (B = 0.099, 95% CI = 0.014, 0.183). ‘Community transportation environment’ was associated with overall children’s average day LPA (B = 4.034, 95% CI = 0.012, 8.056), weekend LPA (B = 8.278, 95% CI = 1.900, 14.657), MPA (B = 4.485, 95% CI = 0.613, 8.357), TPA (B = 14.777, 95% CI = 2.130, 27.424), and FAT for girls (B = −0.223, 95% CI = −0.443, −0.003). Furthermore, ‘community personal safety’ was associated with boys’ weekday VPA (B = −3.012, 95% CI = −5.946, 0.079). Parental perception of appropriate walking distances, improvement of PA facilities, community personal safety, and the community transportation environment all contribute to the prevention of preschool children’s PA patterns deterioration.
Net Primary Productivity Variations Associated with Climate Change and Human Activities in Nanjing Metropolitan Area of China
Authors: Chen Shulin , Yang Li , Liu Xiaotong , Zhu Zhenghao
Published: November 2022

Rapid economic development has changed land use and population density, which in turn affects the stability and carbon sequestration capacity of regional ecosystems. Net primary productivity (NPP) can reflect the carbon sequestration capacity of ecosystems and is affected by both climate change and human activities. Therefore, quantifying the relative contributions of climate change and human activities on NPP can help us understand the impact of climate change and human activities on the carbon sequestration capacity of ecosystems. At present, researchers have paid more attention to the impact of climate change and land use change on NPP. However, few studies have analyzed the response of the NPP to gross domestic product (GDP) and population density variations on a pixel scale. Therefore, this paper analyzes the impact of climate change and human activities to NPP on a pixel scale in the Nanjing metropolitan area. During the period 2000–2019, the annual mean NPP was 494.89 g C·m−2·year−1, and the NPP in the south of the Nanjing metropolitan area was higher than that in the north. The NPP was higher in the forest, followed by unused land, grassland, and cropland. In the past 20 years, the annual mean NPP showed a significant upward trend, with a growth rate of 3.78 g C·m−2·year−1. The increase in temperature and precipitation has led to an increasing trend of regional NPP, and the impact of precipitation on NPP was more significant than that of temperature. The transformation of land use from low-NPP type to high-NPP type also led to an increase in NPP. Land use change from high-NPP type to low-NPP type was the main cause of regional NPP decline. Residual analysis was used to analyze the impact of human activities on NPP. Over the last 20 years, the NPP affected by human activities (NPPhum) showed a high spatial pattern in the south and a low spatial pattern in the north, and the annual mean NPPhum also showed a fluctuating upward trend, with a growth rate of 2.00 g C·m−2·year−1. The NPPhum was influenced by both GDP and population density, and the impact of population density on NPP was greater than that of GDP.
Interprofessional Identity in Health and Social Care: Analysis and Synthesis of the Assumptions and Conceptions in the Literature
Authors: Cantaert Gabriël Rafaël , Pype Peter , Valcke Martin , Lauwerier Emelien
Published: November 2022

Interprofessional identity (IPI) development is considered essential in reducing incongruency and improving interprofessional collaboration. However, noticeable differences in conceptualizations are being put forward in the literature, hindering interpretation of research findings and translation into practice. Therefore, a Concept Analysis and Critical Interpretative Synthesis of empirical research articles were conducted to explore the assumptions and conceptions of IPI. Independent literature screening by two researchers led to the inclusion and extraction of 39 out of 1334 articles. Through critical analysis, higher order themes were constructed and translated to a synthesizing argument and a conceptual framework depicting what constitutes IPI (attributes), the boundary conditions (antecedents) and the outcomes (consequences) of its development. The attributes refer to both IPI’s structural properties and the core beliefs indicative of an interprofessional orientation. The antecedents inform us on the importance of IPI-fitting constructivist learning environments and intergroup leadership in enabling its development. This development may lead to several consequences with regard to professional wellbeing, team effectiveness and the quintuple aim. Given the educational orientation of this study, ways for facilitating and assessing the development of IPI among learners across the professional continuum have been proposed, although empirical research is needed to further validate links and mediating and moderating variables.
Relationship between Attention to Body Shape, Social Physique Anxiety, and Personal Characteristics of Brazilians: A Structural Equation Model
Authors: Silva Wanderson Roberto da , Teixeira Patrícia Angélica , Marôco João , Ferreira Eric Batista , Teodoro Micaela Aparecida , Campos Juliana Alvares Duarte Bonini
Published: November 2022

People can develop eating disorders due to excessive body image concerns. The primary objective of this study was to examine the relationship between attention to body shape, social physique anxiety, and personal characteristics in a sample of Brazilians. The secondary objective was to evaluate the correlation of the constructs with the participants’ body composition. First, 1795 individuals (70% female; Mage = 25.5 ± 6.6 years) completed the Attention to Body Shape Scale, the Social Physique Anxiety Scale, and a sociodemographic questionnaire. Then, 286 participants (58% female; Mage = 25.3 ± 5.7 years) underwent a bioimpedance exam to identify body composition. Structural equation modeling was used to estimate the relationship between the variables. The greater the attention to body shape, the greater the expectations of negative physical evaluation and the less comfort with physical presentation. Younger age, female gender, consumption of supplements/substances for body change, restrictive diets, physical inactivity, poor self-assessment of food quality, and overweight/obesity were related to negative body concerns. An expectation of negative physical evaluation was positively correlated with body fat and negatively with muscle mass. Comfort with physical presentation was negatively correlated with fat and positively with muscle. These results can support preventive strategies aimed at reducing eating disorders resulting from body image concerns.
Global Research Trends on Smart Homes for Older Adults: Bibliometric and Scientometric Analyses
Authors: Hong Yi-Kyung , Wang Ze-Yu , Cho Ji Young
Published: November 2022

A growing aging population across the world signifies the importance of smart homes equipped with appropriate technology for the safety and health of older adults. Well-designed smart homes can increase the desire of older adults’ aging-in-place and bring economic benefits to the country by reducing budgets for care providers. To obtain a structural overview and provide significant insights into the characteristics of smart homes for older adults, this study conducted bibliometric and scientometric analyses. We used the Web of Science Core Collection database, searching for keywords “smart home*”, “home automation”, or “domotics” with terms related to older adults, resulting in a total of 1408 documents. VOSviewer software was used to map and visualize the documents. The results showed that research on smart homes for older adults began appearing from 1997 and increased steadily, peaking from 2015. The main research areas were technical engineering fields, such as computer science and engineering, telecommunications with minimal research in humanities, social sciences, and design, indicating the necessity to expand research toward a human-centered perspective, age-friendly technology, and convergence study.
Assessment of Glucose Lowering Medications’ Effectiveness for Cardiovascular Clinical Risk Management of Real-World Patients with Type 2 Diabetes: Targeted Maximum Likelihood Estimation under Model Misspecification and Missing Outcomes
Authors: Sciannameo Veronica , Fadini Gian Paolo , Bottigliengo Daniele , Avogaro Angelo , Baldi Ileana , Gregori Dario , Berchialla Paola
Published: November 2022

The results from many cardiovascular (CV) outcome trials suggest that glucose lowering medications (GLMs) are effective for the CV clinical risk management of type 2 diabetes (T2D) patients. The aim of this study is to compare the effectiveness of two GLMs (SGLT2i and GLP-1RA) for the CV clinical risk management of T2D patients in a real-world setting, by simultaneously reducing glycated hemoglobin, body weight, and systolic blood pressure. Data from the real-world Italian multicenter retrospective study Dapagliflozin Real World evideNce in Type 2 Diabetes (DARWINT 2D) are analyzed. Different statistical approaches are compared to deal with the real-world-associated issues, which can arise from model misspecification, nonrandomized treatment assignment, and a high percentage of missingness in the outcome, and can potentially bias the marginal treatment effect (MTE) estimate and thus have an influence on the clinical risk management of patients. We compare the logistic regression (LR), propensity score (PS)-based methods, and the targeted maximum likelihood estimator (TMLE), which allows for the use of machine learning (ML) models. Furthermore, a simulation study is performed, resembling the structure of the conditional dependencies among the main variables in DARWIN-T2D. LR and PS methods do not underline any difference in the effectiveness regarding the attainment of combined CV risk factor goals between the two treatments. TMLE suggests instead that dapagliflozin is significantly more effective than GLP-1RA for the CV risk management of T2D patients. The results from the simulation study suggest that TMLE has the lowest bias and SE for the estimate of the MTE.
When COVID-19 Is Not All: Femicide Conducted by a Murderer with a Narcissistic Personality “Masked” by a Brief Psychotic Disorder, with a Mini-Review
Authors: Morena Donato , Di Fazio Nicola , La Russa Raffaele , Delogu Giuseppe , Frati Paola , Fineschi Vittorio , Ferracuti Stefano
Published: November 2022

Several cases of COVID-19-related mental disorders have emerged during the pandemic. In a case of femicide that occurred in Italy during the first phase of the pandemic, coinciding with a national lockdown, a discrepancy arose among forensic psychiatry experts, particularly toward the diagnosis of Brief Psychotic Disorder (BPD) related to COVID-19. We aimed to discuss the evaluation of the case through an integration of information and a literature review on comparable reported cases. An analysis of the diagnosis of brief acute psychosis was then performed, as well as a mini-review on cases of COVID-19-related psychosis. Results showed that psychotic symptomatology was characterized by polythematic delusions that always involved a SARS-CoV-2 infection. To a lesser extent, the delusions were accompanied by hallucinations, bizarre cognitive and associative alterations, insomnia, hyporexia, dysphoria, and suicidal behavior. No particularly violent acts with related injury or death of the victim were described. Finally, we could hypothesize that our case was better represented by a diagnosis of personality with predominantly narcissistic and partly psychopathic traits. The present case highlighted the importance, in the context of forensic psychiatry, of integrating assessments with the crime perpetrators, namely through accurate clinical interviews, neuropsychological tests, diachronic observations, and comparison with similar cases present in the literature. Such an integrated approach allows precise evaluation and reduces the odds of errors in a field, such as forensic psychiatry, where a diagnostic decision can be decisive in the judgment of criminal responsibility. Moreover, discerning forensics from health cases represents an important issue in risk management.
May Sulfonamide Inhibitors of Carbonic Anhydrases from Mammaliicoccus sciuri Prevent Antimicrobial Resistance Due to Gene Transfer to Other Harmful Staphylococci?
Authors: De Luca Viviana , Giovannuzzi Simone , Supuran Claudiu T. , Capasso Clemente
Published: November 2022

Mammaliicoccus sciuri, previously known as Staphylococcus sciuri, is a Gram-positive bacterium involved in gene transfer phenomena that confer resistance to multiple antibiotics. These plasmid-encoded genes can be easily transferred to other pathogenic staphylococci. Because antibiotic resistance is rising, inhibiting M. sciuri proliferation may be a credible strategy for restricting antimicrobial resistance gene transfer to other pathogenic bacteria. Recently, it has been shown that blocking bacterial carbonic anhydrases (CAs, EC 4.2.1.1), metalloenzymes sustaining bacterial metabolic activities, can reduce pathogen survival and fitness. Here, the recombinant M. sciuri γ-CA (MscCAγ) has been cloned and purified, utilizing the DNA recombinant technology. Its kinetic properties for the CO2 hydration reaction, as well as the sulfonamide inhibition profile, were investigated and compared with those reported earlier for MscCAβ (previously described as SauBCA) and the two off-target human CA isoforms (hCA I and hCA II). The recombinant MscCAγ showed significant hydratase activity. Moreover, the MscCAγ sulfonamide inhibitory profile was different from that of MscCAβ, implying that a varied amino acid set typifies the catalytic pocket of the two enzymes. These differences provide additional evidence for the possibility of developing novel CA class-specific inhibitors.
The Role of Cluster C19MC in Pre-Eclampsia Development
Authors: Jaszczuk Ilona , Winkler Izabela , Koczkodaj Dorota , Skrzypczak Maciej , Filip Agata
Published: November 2022

Pre-eclampsia is a placenta-related complication occurring in 2–10% of all pregnancies. miRNAs are a group of non-coding RNAs regulating gene expression. There is evidence that C19MC miRNAs are involved in the development of the placenta. Deregulation of chromosome 19 microRNA cluster (C19MC) miRNAs expression leads to impaired cell differentiation, abnormal trophoblast invasion and pathological angiogenesis, which can lead to the development of pre-eclampsia. Information was obtained through a review of articles available in PubMed Medline. Articles on the role of the C19MC miRNA in the development of pre-eclampsia published in 2009–2022 were analyzed. This review article summarizes the current data on the role of the C19MC miRNA in the development of pre-eclampsia. They indicate a significant increase in the expression of most C19MC miRNAs in placental tissue and a high level of circulating fractions in serum and plasma, both in the first and/or third trimester in women with PE. Only for miR-525-5p, low levels of plasma expression were noted in the first trimester, and in the placenta in the third trimester. The search for molecular factors indicating the development of pre-eclampsia before the onset of clinical symptoms seems to be a promising diagnostic route. Identifying women at risk of developing pre-eclampsia at the pre-symptomatic stage would avoid serious complications in both mothers and fetuses. We believe that miRNAs belonging to cluster C19MC could be promising biomarkers of pre-eclampsia development.
Chronic Methylmercury Intoxication Induces Systemic Inflammation, Behavioral, and Hippocampal Amino Acid Changes in C57BL6J Adult Mice
Authors: Nascimento Tyciane S. , Pinto Daniel V. , Dias Ronaldo P. , Raposo Ramon S. , Nunes Paulo Iury G. , Roque Cássia R. , Santos Flávia A. , Andrade Geanne M. , Viana José Lucas , Fostier Anne H. , Sussulini Alessandra , Alvarez-Leite Jacqueline I. , Fontes-Ribeiro Carlos , Malva João O. , Oriá Reinaldo B.
Published: November 2022

Methylmercury (MeHg) is highly toxic to the human brain. Although much is known about MeHg neurotoxic effects, less is known about how chronic MeHg affects hippocampal amino acids and other neurochemical markers in adult mice. In this study, we evaluated the MeHg effects on systemic lipids and inflammation, hippocampal oxidative stress, amino acid levels, neuroinflammation, and behavior in adult male mice. Challenged mice received MeHg in drinking water (2 mg/L) for 30 days. We assessed weight gain, total plasma cholesterol (TC), triglycerides (TG), endotoxin, and TNF levels. Hippocampal myeloperoxidase (MPO), malondialdehyde (MDA), acetylcholinesterase (AChE), amino acid levels, and cytokine transcripts were evaluated. Mice underwent open field, object recognition, Y, and Barnes maze tests. MeHg-intoxicated mice had higher weight gain and increased the TG and TC plasma levels. Elevated circulating TNF and LPS confirmed systemic inflammation. Higher levels of MPO and MDA and a reduction in IL-4 transcripts were found in the hippocampus. MeHg-intoxication led to increased GABA and glycine, reduced hippocampal taurine levels, delayed acquisition in the Barnes maze, and poor locomotor activity. No significant changes were found in AChE activity and object recognition. Altogether, our findings highlight chronic MeHg-induced effects that may have long-term mental health consequences in prolonged exposed human populations.
Carvacrol Inhibits Expression of Transient Receptor Potential Melastatin 7 Channels and Alleviates Zinc Neurotoxicity Induced by Traumatic Brain Injury
Authors: Lee Minwoo , Lee Song Hee , Choi Seunghyuk , Choi Bo Young , Suh Sang Won
Published: November 2022

Carvacrol is a monoterpenoid phenol produced by aromatic plants such as oregano. Although the exact mechanism by which carvacrol acts has not yet been established, it appears to inhibit transient receptor potential melastatin 7 (TRPM7), which modulates the homeostasis of metal ions such as zinc and calcium. Several studies have demonstrated that carvacrol has protective effects against zinc neurotoxicity after ischemia and epilepsy. However, to date, no studies have investigated the effect of carvacrol on traumatic brain injury (TBI)-induced zinc neurotoxicity. In the present study, we investigated the therapeutic potential of carvacrol for the prevention of zinc-induced neuronal death after TBI. Rats were subjected to a controlled cortical impact, and carvacrol was injected at a dose of 50 mg/kg. Histological analysis was performed at 12 h, 24 h, and 7 days after TBI. We found that carvacrol reduced TBI-induced TRPM7 over-expression and free zinc accumulation. As a result, subsequent oxidative stress, dendritic damage, and neuronal degeneration were decreased. Moreover, carvacrol not only reduced microglial activation and delayed neuronal death but also improved neurological outcomes after TBI. Taken together, these findings suggest that carvacrol administration may have therapeutic potential after TBI by preventing neuronal death through the inhibition of TRPM7 expression and alleviation of zinc neurotoxicity.
The Correlation of Ten Immune Checkpoint Gene Expressions and Their Association with Gastric Cancer Development
Authors: Mansorunov Danzan , Apanovich Natalya , Kipkeeva Fatimat , Nikulin Maxim , Malikhova Olga , Stilidi Ivan , Karpukhin Alexander
Published: November 2022

In the immunotherapy based on immune checkpoint inhibition (IC), additional ICs are being studied to increase its effectiveness. An almost unstudied feature is the possible co-expression of ICs, which can determine the therapeutic efficacy of their inhibition. For the selection of promising ICs, information on the association of their expression with cancer development may be essential. We have obtained data on the expression correlation of ADAM17, PVR, TDO2, CD274, CD276, CEACAM1, IDO1, LGALS3, LGALS9, and HHLA2 genes in gastric cancer (GC). All but one, TDO2, have other IC genes with co-expression at some stage. At the metastatic stage, the expression of the IDO1 does not correlate with any other gene. The correlations are positive, but the expressions of the CD276 and CEACAM1 genes are negatively correlated. The expression of TDO2 and LGALS3 is associated with GC metastasis. The expression of TDO2 four-fold higher in metastatic tumors than in non-metastatic tumors, but LGALS3 was two-fold lower. The differentiation is associated with IDO1. The revealed features of TDO2, with a significant increase in expression at the metastatic stage and the absence of other IC genes with correlated expression indicates that the prospect of inhibiting TDO2 in metastatic GC. IDO1 may be considered for inhibition in low-differentiated tumors.
Comparative Seeds Storage Transcriptome Analysis of Astronium fraxinifolium Schott, a Threatened Tree Species from Brazil
Authors: Pereira Neto Leonel Gonçalves , Rossini Bruno Cesar , Marino Celso Luis , Toorop Peter E. , Silva Edvaldo Aparecido Amaral
Published: November 2022

Astronium fraxinifolium Schott (Anacardiaceae), also known as a ‘gonçalo-alves’, is a tree of the American tropics, with distribution in Mexico, part of Central America, Argentina, Bolivia, Brazil and Paraguay. In Brazil it is an endangered species that occurs in the Cerrado, Caatinga and in the Amazon biomes. In support of ex situ conservation, this work aimed to study two accessions with different longevity (p50) of A. fraxinifolium collected from two different geographic regions, and to evaluate the transcriptome during aging of the seeds in order to identify genes related to seed longevity. Artificial ageing was performed at a constant temperature of 45 °C and 60% relative humidity. RNA was extracted from 100 embryonic axes exposed to control and aging conditions for 21 days. The transcriptome analysis revealed differentially expressed genes such as Late Embryogenesis Abundant (LEA) genes, genes involved in the photosystem, glycine rich protein (GRP) genes, and several transcription factors associated with embryo development and ubiquitin-conjugating enzymes. Thus, these results contribute to understanding which genes play a role in seed ageing, and may serve as a basis for future functional characterization of the seed aging process in A. fraxinifolium.
Advanced Sperm Selection Strategies as a Treatment for Infertile Couples: A Systematic Review
Authors: Ribas-Maynou Jordi , Barranco Isabel , Sorolla-Segura Maria , Llavanera Marc , Delgado-Bermúdez Ariadna , Yeste Marc
Published: November 2022

Assisted reproductive technology (ART) is an essential tool to overcome infertility, and is a worldwide disease that affects millions of couples at reproductive age. Sperm selection is a crucial step in ART treatment, as it ensures the use of the highest quality sperm for fertilization, thus increasing the chances of a positive outcome. In recent years, advanced sperm selection strategies for ART have been developed with the aim of mimicking the physiological sperm selection that occurs in the female genital tract. This systematic review sought to evaluate whether advanced sperm selection techniques could improve ART outcomes and sperm quality/functionality parameters compared to traditional sperm selection methods (swim-up or density gradients) in infertile couples. According to preferred reporting items for systematic reviews and meta-analyses (PRISMA guidelines), the inclusion and exclusion criteria were defined in a PICOS (population, intervention, comparator, outcome, study) table. A systematic search of the available literature published in MEDLINE-PubMed until December 2021 was subsequently conducted. Although 4237 articles were recorded after an initial search, only 47 studies were finally included. Most reports (30/47; 63.8%) revealed an improvement in ART outcomes after conducting advanced vs. traditional sperm selection methods. Among those that also assessed sperm quality/functionality parameters (12/47), there was a consensus (10/12; 83.3%) about the beneficial effect of advanced sperm selection methods on these variables. In conclusion, the application of advanced sperm selection methods improves ART outcomes. In spite of this, as no differences in the reproductive efficiency between advanced methods has been reported, none can be pointed out as a gold standard to be conducted routinely. Further research addressing whether the efficiency of each method relies on the etiology of infertility is warranted.
Overexpression of Ubiquitin-Conjugating Enzyme E2C Is Associated with Worsened Prognosis in Prostate Cancer
Authors: Wu Xiaobo , Long Xingbo , Ma Chenkai , Cheuk Yin Celeste , Hu Mengbo , Hu Jimeng , Jiang Haowen
Published: November 2022

To evaluate the role of ubiquitin-conjugating enzyme E2C (UBE2C) in prostate cancer (PCa) progression and prognosis, the TCGA and our PCa tissue microarray cohort were included in the study. Weighted gene co-expression network analysis (WGCNA) and non-negative matrix factorization were used to cluster patients and to screen genes that play a vital role in PCa progression (hub gene). Immunohistochemistry staining was used to evaluate the protein level of UBE2C in prostatic tissues. Through WGCNA, we found a gene co-expression module (named the purple module) that is strongly associated with the Gleason score, pathologic T stage, and biochemical recurrent status. Genes in the purple module are enriched in cell cycle and P53 signaling and help us to cluster patients into two groups with distinctive biochemical recurrent survival rates and TP53 mutation statuses. Further analysis showed UBE2C served as a hub gene in the purple module. The expression of UBE2C in PCa was significantly higher than that in paracancerous tissues and was remarkably associated with pathologic grade, Gleason score, and prognosis in PCa patients. To conclude, UBE2C is a PCa-progress-related gene and a biomarker for PCa patients. Therapy targeting UBE2C may serve as a promising treatment of PCa in the future.