bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of high intensity interval and moderate continuous training on plasma ratios of ProBNP1-108/BNP1-32 and NT-pro-BNP1-76/BNP1-32 after coronary artery bypass grafting surgery
Authors: Zare Karizak Sara , Kashef Majid , Gaeini Abbas Ali , Nejatian Mostafa
Published: March 2023

Background: ProBNP1-108/BNP1-32, and NT-pro-BNP1-76/BNP1-32 ratios are significant indices for predicting complications after coronary artery bypass grafting (CABG) surgery. However, the effect of aerobic training types on these biomarkers has not been fully understood. So, the current study aimed to determine the impact of aerobic interval and continuous training programs on plasma ratios of ProBNP1-108/BNP1-32 and NT-pro-BNP1-76/BNP1-32 after coronary artery bypass grafting surgery.

Method: 36 patients were selected purposive (27 men and 9 women with mean of age 60.32 ± 5.81 years, height 164.64 ± 9.25 cm, weight 73.86 ± 14.23 kg, fat 32.30 ± 4.28, SBP 142.67 ± 6.49, DBP 84.5 ± 5.16 mmHg in seated position at rest situation and functional capacity of 7.08 ± 2.49 METs) and then divided randomly into three groups: control (C) group (without training program) moderate continuous training (MCT) and high intensity interval training (HIIT) (exercise training program was performed 3 days/week for 8 weeks) with intensities 65%–80% and 80%–95% of reserve heart rate in order. Blood samples were taken 48 h before the first session and 48 h after the last training session to measure the plasma levels of ProBNP1–108, corin enzyme, BNP1-32, and NT-pro-BNP1-76 using the enzyme-linked immunosorbent assay (ELISA) technique. Wilcoxin and kruskal wallis tests were used for analyzing data.

Results: The plasma corin enzyme was increased, and the ratios of proBNP1-108/BNP1-32 and NT-pro-BNP1-76/BNP1-32 were reduced in both training groups in compared with control group (p = 0.004, p = 0000, p = 0.016, p = 0.003, p = 0.009, and p = 0.016) when there was no significant difference was found between training groups (p = 0.074, p = 450, and p = 0.295).

Conclusion: Both high intensity interval training and moderate continuous training in compared with inactivity have positive effects on ratios of ProBNP1-108/BNP1-32, NT-pro-BNP1-76/BNP1-32 and could be effective to promote the health of coronary arteries and prevention of HF in post-CABG patients.
Developmental dynamic transcriptome and systematic analysis reveal the major genes underlying isoflavone accumulation in soybean
Authors: Chen Heng , Liu Changkai , Li Yansheng , Wang Xue , Pan Xiangwen , Wang Feifei , Zhang Qiuying
Published: March 2023

Introduction

Soy isoflavone, a class of polyphenolic compounds exclusively occurred in legumes, is an important bioactive compound for both plants and human beings. The outline of isoflavones biosynthesis pathway has been drawn up basically in the previous research. However, research on the subject has been mostly restricted to investigate the static regulation of isoflavone content in soybean, rather than characterize its dynamic variation and modulation network in developing seeds.

Methods

In this study, by using eight recombinant inbred lines (RIL), the contents of six isoflavone components in the different stages of developing soybean seeds were determined to characterize the dynamic variation of isoflavones, and the isoflavones accumulation pattern at physiological level was investigated. Meanwhile, we integrated and analyzed the whole genome expression profile of four lines and 42 meta-transcriptome data, based on the multiple algorithms.

Results

This study: 1) obtained 4 molecular modules strongly correlated with isoflavone accumulation; 2) identified 28 novel major genes that could affect the accumulation of isoflavones in developing seeds free from the limitation of environments; 3) discussed the dynamic molecular patterns regulating isoflavones accumulation in developing seed; 4) expanded the isoflavone biosynthesis pathway.

Discussion

The results not only promote the understandings on the biosynthesis and regulation of isoflavones at physiological and molecular level, but also facilitate to breed elite soybean cultivars with high isoflavone contents.
Root traits in response to frequent fires: Implications for belowground carbon dynamics in fire-prone savannas
Authors: Zhou Yong
Published: March 2023

Predicting how belowground carbon storage reflects changes in aboveground vegetation biomass is an unresolved challenge in most ecosystems. This is especially true for fire-prone savannas, where frequent fires shape the fraction of carbon allocated to root traits for post-fire vegetation recovery. Here I review evidence on how root traits may respond to frequent fires and propose to leverage root traits to infer belowground carbon dynamics in fire-prone savannas. Evidently, we still lack an understanding of trade-offs in root acquisitive vs. conservative traits in response to frequent fires, nor have we determined which root traits are functionally important to mediate belowground carbon dynamics in a frequently burned environment. Focusing research efforts along these topics should improve our understanding of savanna carbon cycling under future changes in fire regimes.
Age effects of Moso bamboo on leaf isoprene emission characteristics
Authors: Song Yandong , Peng Chunju , Wu Qinjiao , Tao Shijie , Mei Tingting , Sun Zhihong , Zuo Zhaojiang , Pan Chunyu , Zhou Yufeng , Zhou Guomo
Published: March 2023

Isoprene is a highly reactive volatile organic compound that significantly affects atmospheric oxidant capacity, regional air quality, and climate change. Moso bamboo (Phyllostachys edulis), a species widely distributed in tropical and subtropical regions, particularly in China, is a strong isoprene emitter with great potential for carbon sequestration. Carbon sequestration is negatively correlated with culm age; however, the effect of this correlation on isoprene emissions remains unknown. In this study, we investigated the photosynthetic and isoprene emission characteristics of Moso bamboo at different culm ages. The results showed that the age effect on isoprene emission was different from that on photosynthesis; the net photosynthesis rate (Pn) was the highest in young, followed by mature, and then old bamboo, whereas the isoprene emission rate (Iso) was the highest in young, followed by old, and then mature bamboo. Moreover, the percentage of carbon loss as isoprene emission (C-loss) during photosynthesis of old bamboo was 35% higher than that of mature bamboo under standard conditions (leaf temperature: 30°C; light intensity: 1000 µmol m-2 s-1). Therefore, we strongly recommend considering the culm age when establishing an isoprene emission model of Moso bamboo. Additionally, because the Iso and C-loss of old bamboo were higher than those of mature bamboo, we suggest that attention should be paid to the management of bamboo age structure and timely felling of aged bamboo to reduce environmental risk.
The original polyethylene microplastics inhibit the growth of sweet potatoes and increase the safety risk of cadmium
Authors: Shi Liang , Chen Zanming , Hou Yanan , Li Jianmin , Shen Zhenguo , Chen Yahua
Published: March 2023

Microplastics (MPs) and heavy metals (HMs) co-exist in sweet potato fields of China. As the main component of agricultural field mulch and one of the most polluting and harmful HMs, the effects of polyethylene microplastics (PE MPs) and cadmium (Cd) on sweet potato and soil environment are remains unclear. Here, pot and hydroponic experiments are used to explore the effects of original and weathered PE MPs on growth and Cd uptake of sweet potatoes. The results of pot experiments reveal that compared with the control (0%), 5% of weathered PE MPs can significantly increase soil electrical conductivity (EC); both 5% of the original PE MPs and weathered PE MPs can significantly reduce the concentration of Olsen phosphorus (P) and Olsen potassium (K) in soil, inhibit plant growth, but significantly increase Cd accumulation and glutathione (GSH) level in tissues of sweet potatoes, and also induce membrane lipid peroxidation. In addition, compared to 5% weathered PE MPs, 5% original PE MPs significantly reduce soil EC, growth and peroxidase level of sweet potatoes, but significantly increase Cd concentration in leaves and stems. The results of hydroponic experiment show that original PE MPs significantly increase the P, K, and Cd adsorption compared with weathered PE MPs, and Cd increases the original PE MPs accumulation in the root cortex but decrease PE MPs accumulation in shoots. To sum up, our study investigates the differences and reasons of the effects of original and weathered PE MPs on growth and Cd absorption of sweet potatoes.
Recommendations for cyberbullying prevention and intervention: A Western Canadian perspective from key stakeholders
Authors: Hendry Brittany P. , Hellsten Laurie-ann Michelle , McIntyre Laureen J. , Smith Brenan R. R.
Published: March 2023

Introduction

Cyberbullying, or repeatedly communicating antagonistic messages using digital or electronic media meant to deal out harm or discomfort to others, has been considered more pervasive and impactful than traditional bullying since perpetrators can remain anonymous online, are not bound by time or place. In addition, cyberbullied youth are reluctant to involve others such as an adult or confront the perpetrator adults. Therefore, the primary purpose of this study was to capture a holistic understanding of potential youth cyberbullying prevention and intervention strategies (i.e., inhibiting forces that may reduce cyberbullying) from key stakeholders with professional knowledge about cyberbullying (i.e., educational administration, psychological counseling, technology and bullying education consultation, policing, research, and social support services).

Methods

Twenty (n = 20) participants were recruited using purposive and snowball sampling techniques from both urban and rural school districts in one Western Canadian province to participate in either in a semi-structured individual interview (n = 16) or a scheduled focus group (n = 4) to achieve depth and understanding of cyberbullying issues. The I3 Model, a process-oriented metatheory of aggression with the potential to explain how cyberbullying behaviors continue to occur, was used as a frame to analyze the qualitatively gathered data using six phases of reflexive thematic analysis.

Results

Participants identified educational efforts related to awareness of cyberbullying and consequences of perpetration, digital citizenship programming for students and social skills training, providing remediation to youth who are in online conflict with one another, and parental engagement with the technology used by their youth as key factors in mitigating instances of cyberbullying.

Discussion

This study furthers research on cyberbullying prevention and intervention in schools by illuminating experiences from under researched and unique stakeholders in the field. These key findings and suggestions for future research are further discussed.
Gender differences in behavioral inhibitory control under evoked acute stress: An event-related potential study
Authors: Di Siyu , Ma Chao , Wu Xiaoguang , Lei Liang
Published: March 2023

Purpose

This study investigated gender differences in behavioral inhibitory control among college students under acute stress state by using event-related potential technique.

Methods

Acute stress was evoked in 41 college students (22 males and 19 females) using the Trier Social Stress paradigm, and the neutral state was matched using out-of-speech reading, with subjects completing a two-choice Oddball task in each of the two states. In combination with the ERP technique, the area under the stress curve, reaction time, number of errors, and the difference waves between the two stimulus conditions in the frontal-central region N2 wave amplitude and the parietal-central region P3 wave amplitude were compared between the two groups of subjects in the stressful and neutral state.

Results

The results revealed that the area under the stress curve was larger under the stress condition compared to the neutral condition, and the area under the stress curve was larger in females than in males. Behavioral results showed no statistically significant differences in reaction time and number of errors between the two genders in the acute stress condition. The ERP results showed that the wave amplitudes of N2 and P3 decreased significantly in both genders in the acute stress state. The decrease in N2 amplitude was greater in females during the transition from neutral to stressful condition, while the difference in P3 amplitude was not statistically significant in both genders.

Conclusion

The findings suggest that evoked acute stress can promote behavioral inhibitory control in both genders and that females are more sensitive to acute stress state.
The impact of an organizational-level mindfulness-based intervention on workplace social capital and psychological safety: A qualitative content analysis
Authors: Bonde Emilie Hasager , Mikkelsen Eva Gemzøe , Fjorback Lone Overby , Juul Lise
Published: March 2023

Background

Through the past decades, the mental health of the European population has been continuously declining. Social relations in various spheres of life, including workplace settings, have been shown to impact mental health. Mindfulness-based stress reduction (MBSR) has been found effective in enhancing well-being, and reducing perceived stress, and symptoms of depression and anxiety. Research into mindfulness-based interventions (MBIs) in workplace settings has shown that these interventions may positively affect workplace outcomes, such as interpersonal relations. However, research regarding the organizational impacts of MBIs is still nascent. The objective of this study was to investigate how an organizational-level mindfulness-based intervention (MBI) including a workplace-adapted MBSR programme may impact workplace social capital and psychological safety.

Methods

Four small and medium-sized private companies were included in this study, representing 368 employees and managers. The intervention consisted of three steps: 1. Mandatory participation in introductory sessions on mental health and mindfulness, 2. Voluntary participation in a 10-week workplace-adapted MBSR programme, and 3. A workshop for selected employee representatives and managers on further implementation of mindfulness. Data was collected using pre and post-intervention focus group interviews. In total, 27 interviews including 76 respondents were conducted. Verbatim transcription was performed. Data was analyzed using deductive content analysis with theoretical frameworks for social capital and psychological safety.

Results

The analysis resulted in three main categories: 1. Social capital (1.1. bonding social capital, 1.2. bridging social capital, 1.3. linking social capital), 2. Psychological safety, and 3. Emergent theme: The role of lockdown on the perceived organizational impact of a workplace MBI. The greatest impact was found relating to the bridging social capital, i.e., social capital between departments, and psychological safety among colleagues at the same level of employment.

Conclusion

The results indicate that company participation in this organizational-level MBI including a workplace-adapted MBSR programme may positively impact social relations at work, especially the bridging social capital and psychological safety between colleagues at the same level of employment. These results may have been influenced by lockdowns due to the COVID-19 pandemic.
Improving middle school students’ geometry problem solving ability through hands-on experience: An fNIRS study
Authors: Shi Licheng , Dong Linwei , Zhao Weikun , Tan Dingliang
Published: March 2023

Hands-on learning is proposed as a prerequisite for mathematics learning in kindergarten and primary school. However, it remains unclear that whether hands-on experience aids understanding of geometry knowledge for middle school students. We also know little about the neural basis underlying the value of hands-on experience in math education. In this study, 40 right-handed Chinese students (20 boys and 20 girls) with different academic levels were selected from 126 seventh-grade students in the same school, who learnt “Axisymmetric of an Isosceles Triangle” in different learning style (hands-on operation vs. video observation). Half of them operated the concrete manipulatives while the other half watched the instructional videos. The learning-test paradigm and functional near-infrared spectroscopy (fNIRS) technique were used to compare the differences in geometry reasoning involved in solving well-structured problems and ill-structured problems. Behavioral results showed that hands-on experience promoted students’ performances of geometry problem-solving. Students with lower academic level were more dependent on hands-on experience than those with higher academic level. The fNIRS results showed that meaningful hands-on experience with concrete manipulatives related to learning contents increased reactivation of the somatosensory association cortex during subsequent reasoning, which helped to improve the problem-solving performance. Hands-on experience also reduced students’ cognitive load during the well-structured problem-solving process. These findings contribute to better understand the value of hands-on experience in geometry learning and the implications for future mathematics classroom practices.
Development of cannabis use disorder in medical cannabis users: A 9-month follow-up of a randomized clinical trial testing effects of medical cannabis card ownership
Authors: Cooke Megan E. , Potter Kevin W. , Jashinski Julia , Pascale Michael , Schuster Randi M. , Tervo-Clemmens Brenden , Hoeppner Bettina B. , Pachas Gladys N. , Evins A. Eden , Gilman Jodi M.
Published: March 2023

Background

Evidence for long-term effectiveness of commercial cannabis products used to treat medical symptoms is inconsistent, despite increasingly widespread use.

Objective

To prospectively evaluate the effects of using cannabis on self-reported symptoms of pain, insomnia, anxiety, depression, and cannabis use disorder (CUD) after 12 months of use.

Methods

This observational cohort study describes outcomes over 9 months following a 12-week randomized, waitlist-controlled trial (RCT: NCT03224468) in which adults (N = 163) who wished to use cannabis to alleviate insomnia, pain, depression, or anxiety symptoms were randomly assigned to obtain a medical marijuana card immediately (immediate card acquisition group) or to delay obtaining a card for 12 weeks delay (delayed card acquisition group). During the 9-month post-randomization period, all participants could use cannabis as they wished and choose their cannabis products, doses, and frequency of use. Insomnia, pain, depression, anxiety, and CUD symptoms were assessed over the 9-month post-randomization period.

Results

After 12 months of using cannabis for medical symptoms, 11.7% of all participants (n = 19), and 17.1% of those using cannabis daily or near-daily (n = 6) developed CUD. Frequency of cannabis use was positively correlated with pain severity and number of CUD symptoms, but not significantly associated with severity of self-reported insomnia, depression, or anxiety symptoms. Depression scores improved throughout the 9 months in all participants, regardless of cannabis use frequency.

Conclusions

Frequency of cannabis use was not associated with improved pain, anxiety, or depression symptoms but was associated with new-onset cannabis use disorder in a significant minority of participants. Daily or near-daily cannabis use appears to have little benefit for these symptoms after 12 months of use.
Sex chromosome and gonadal hormone contributions to binge-like and aversion-resistant ethanol drinking behaviors in Four Core Genotypes mice
Authors: Sneddon Elizabeth A. , Masters Brianna M. , Ream Kiara D. , Fennell Kaila A. , DeMedio Jenelle N. , Cash Miranda M. , Hollingsworth Brynn P. , Pandrangi Sai , Thach Chloe M. , Shi Haifei , Radke Anna K.
Published: March 2023

Introduction

While substantial research has focused on the contribution of sex hormones to driving elevated levels of alcohol drinking in female rodents, fewer studies have investigated how genetic influences may underlie sex differences in this behavior.

Methods

We used the Four Core Genotypes (FCG) mouse model to explore the contribution of sex chromosome complement (XX/XY) and gonad type [ovaries (Sry–)/testes (Sry+)] to ethanol (EtOH) consumption and quinine-resistant drinking across two voluntary self-administration tasks: limited access consumption in the home cage and an operant response task.

Results

For limited access drinking in the dark, XY/Sry + (vs. XX/Sry +) mice consumed more 15% EtOH across sessions while preference for 15% EtOH vs. water was higher in XY vs. XX mice regardless of gonad type. XY chromosomes promoted quinine-resistant drinking in mice with ovaries (Sry–) and the estrous cycle did not affect the results. In the operant response task, responding for EtOH was concentration dependent in all genotypes except XX/Sry + mice, which maintained consistent response levels across all concentrations (5–20%) of EtOH. When increasing concentrations of quinine (100–500 μM) were added to the solution, FCG mice were insensitive to quinine-punished EtOH responding, regardless of sex chromosome complement. Sry + mice were further found to be insensitive to quinine when presented in water. Importantly, these effects were not influenced by sensitivity to EtOH’s sedative effect, as no differences were observed in the time to lose the righting reflex or the time to regain the righting reflex between genotypes. Additionally, no differences in EtOH concentration in the blood were observed between any of the genotypes once the righting reflex was regained.

Discussion

These results provide evidence that sex chromosome complement regulates EtOH consumption, preference, and aversion resistance and add to a growing body of literature suggesting that chromosomal sex may be an important contributor to alcohol drinking behaviors. Examination of sex-specific genetic differences may uncover promising new therapeutic targets for high-risk drinking.
Working in corona-designated departments in a fortified underground hospital: Concerns about corona and predictors of job burnout
Authors: Nashashibi Lauren , Khouri Marlyn , Meretyk Irit , Livni Tom , Cohen Noga , Fruchter Eyal
Published: March 2023

Background

In August 2020 during Israel’s second COVID-19 wave Rambam Medical Center opened the Sammy Ofer Fortified Underground Emergency Hospital. This was declared a regional Corona center in the north of Israel, receiving the most severe Corona patients from the region. Alongside the advanced inpatient capacity and technology within the underground facility, there was a severe shortage of trained medical and paramedical staff, as well as harsh working conditions. The current study examined the implications and effects of working in an underground facility on healthcare workers, focusing on emotion regulation tendencies and profession as predictors of job burnout.

Methods

Seventy-six healthcare workers, who had worked in the underground hospital for a minimum continuous period of 2 weeks during the peak of the COVID-19 pandemic, and a control group of 40 healthcare workers from northern Israel were asked to fill out an online survey administered via Qualtrics (total sample 116). The survey comprised six questionnaires: a demographic survey questionnaire; a COVID-19 concerns questionnaire; a psychological distress questionnaire (DASS, Depression Anxiety Stress Scale); trait worry (PSWQ; Penn State Worry Questionnaire); emotion regulation (ERQ, Emotion Regulation Questionnaire), and burnout (SMBM, Shirom - Melamed Burnout Measure).

Results

Independent-samples t-tests revealed no significant differences in psychological distress or burnout between Rambam Underground hospital workers and the control group. Conversely, COVID-19 concern scores were significantly different in the two groups, the Rambam hospital workers showing less concern (M = 2.9, SD = 0.73) than the control group (M = 3.47, SD = 0.76) [t(114) = −3.974, p < 0.001]. Hierarchical linear regression analysis identified the significant predictors of burnout among healthcare workers. Participants’ profession (physician), psychological distress (total DASS score), and a personality trait of worry were statistically significant predictors for job burnout (p = 0.028, p < 0.001, p = 0.023, respectively). Concerns about COVID-19 marginally predicted job burnout (p = 0.09). Group (underground vs. control) and emotion regulation tendencies did not predict burnout.

Conclusion

The two groups showed no significant differences in psychological distress nor in burnout. Being a physician, having an intrinsic trait of being overly worried and experiencing psychological distress were significant predictors for job burnout among healthcare workers, regardless of work environment (underground vs. control).
Experiences of coercion amongst involuntary mental health care users in KwaZulu-Natal, South Africa
Authors: Shozi Zinhle , Saloojee Shamima , Mashaphu Sibongile
Published: March 2023

Background

Involuntary admission is a common practice globally. Previous international studies reported that patients experienced high levels of coercion, threats and a range of negative emotions. Little is known about the patients’ experience in South Africa. The aim of this study was to describe the patient’s experiences of involuntary admission at two psychiatric hospitals in KwaZulu-Natal.

Methods

A cross-sectional descriptive quantitative study of patients admitted involuntarily was conducted. Demographic information was extracted from clinical records and interviews were conducted with consenting participants at discharge. The MacArthur Perceived Coercion Scale, the MacArthur Negative Pressures Scale, and the MacArthur Procedural Justice Scale, of the MacArthur Admission Experience Survey (short form) were utilized to describe participants’ experiences.

Results

This study comprised 131 participants. The response rate was 95.6%. Most participants (n = 96; 73%) experienced high levels of coercion and threats (n = 110; 84%) on admission. About half (n = 61; 46.6%) reported that they felt unheard. Participants reported feeling sad (n = 68; 52%), angry (n = 54; 41.2%), and confused (n = 56; 42.7%). There was a significant association between good insight and a feeling of relief (p = 0.001), and between poor insight and feelings of anger (p = 0.041).

Conclusion

The findings of this study confirm that most patients who were admitted involuntarily experienced high levels of coercion, threats, and exclusion from the decision-making process. Patient involvement and control of the decision-making process must be facilitated to improve clinical and overall health outcomes. The need for involuntary admission must justify the means.
Cost-effectiveness analysis of vaccination against COVID-19 in China
Authors: Zhou Huixuan , Ding Ningxin , Han Xueyan , Zhang Hanyue , Liu Zeting , Jia Xiao , Yu Jingjing , Zhang Wei
Published: March 2023

Introduction

Since September 2020, Chinese populations aged > 3 years have been encouraged to receive a two-dose inoculation with vaccines against coronavirus disease 2019 (COVID-19). This study aims to evaluate the cost-effectiveness of the current vaccination strategy amongst the general population in mainland China from a societal perspective.

Methods

In this study, we construct a decision tree with Markov models to compare the economic and health consequences of the current vaccination strategy versus a no-vaccination scenario, over a time horizon of one year and an annual discount rate of 5%. Transition probabilities, health utilities, healthcare costs, and productivity losses are estimated from literature. Outcome measures include infection rates, death rates, quality-adjusted life years (QALYs), and costs. The incremental cost-effectiveness ratio (ICER) is then calculated to evaluate the cost-effectiveness of the current vaccination strategy, and both one-way deterministic sensitivity analysis and probabilistic sensitivity analysis (PSA) are applied to assess the impact of uncertainties on results.

Results

Our simulation indicates that compared with a no-vaccination scenario, vaccination amongst the general population in mainland China would reduce the infection rate from 100% to 45.3% and decrease the death rate from 6.8% to 3.1%. Consequently, the strategy will lead to a saving of 37,664.77 CNY (US$5,256.70) and a gain of 0.50 QALYs per person per year on average (lifetime QALY and productivity loss due to immature death are included). The cost-saving for each QALY gain is 74,895.69 CNY (US$10,452.85). Result of the PSA indicates that vaccination is the dominating strategy with a probability of 97.9%, and the strategy is cost-effective with a probability of 98.5% when the willingness-to-pay (WTP) is 72,000 CNY (US$10,048.71) per QALY.

Conclusion

Compared with a no-vaccination scenario, vaccination among the general population in mainland China is the dominating strategy from a societal perspective. The conclusion is considered robust in the sensitivity analyses.
Impact of inactivated vaccines on decrease of viral RNA levels in individuals with the SARS-CoV-2 Omicron (BA.2) variant: A retrospective cohort study in Shanghai, China
Authors: Yang Peng , Dang Bianli , Kang Wen , Li Xiaofeng , Wang Tianping , Li Ruijuan , Peng Meijuan , Liu Yushen , Wang Linxu , Cheng Yan , Yu Suhuai , Wei Min , Gao Han , Kang Wenzhen , Shang Lei
Published: March 2023

Background

SARS-CoV-2 Omicron (BA.2) has stronger infectivity and more vaccine breakthrough capability than previous variants. Few studies have examined the impact of inactivated vaccines on the decrease of viral RNA levels in individuals with the Omicron variant, based on individuals' continuous daily cycle threshold (Ct) values and associated medical information from the infection to hospital discharge on a large population.

Methods

We extracted 39,811 individuals from 174,371 Omicron-infected individuals according to data inclusion and exclusion criteria. We performed the survival data analysis and Generalized Estimating Equation to calculate the adjusted relative risk (aRR) to assess the effect of inactivated vaccines on the decrease of viral RNA levels.

Results

Negative conversion was achieved in 54.7 and 94.3% of all infected individuals after one and 2 weeks, respectively. aRRs were shown weak effects on turning negative associated with vaccinations in asymptomatic infections and a little effect in mild diseases. Vaccinations had a protective effect on persistent positivity over 2 and 3 weeks. aRRs, attributed to full and booster vaccinations, were both around 0.7 and had no statistical significance in asymptomatic infections, but were both around 0.6 with statistical significance in mild diseases, respectively. Trends of viral RNA levels among vaccination groups were not significant in asymptomatic infections, but were significant between unvaccinated group and three vaccination groups in mild diseases.

Conclusion

Inactivated vaccines accelerate the decrease of viral RNA levels in asymptomatic and mild Omicron-infected individuals. Vaccinated individuals have lower viral RNA levels, faster negative conversion, and fewer persisting positive proportions than unvaccinated individuals. The effects are more evident and significant in mild diseases than in asymptomatic infections.
A New Hope? Mediterranean Algae Mats Are Thriving With Life
Authors: Rossbach Felix I. , Casoli Edoardo , Beck Milan , Wild Christian
Published: March 2023

Our oceans are full of life and home to many different species. High species diversity often concentrates in specific areas called “biodiversity hotspots” (e.g., coral reefs). These hotspots develop with the help of a few key engineering species (e.g., corals). In the Mediterranean Sea, well-known biodiversity hotspots are seagrass meadows. Macroalgae beds represent another typical habitat but usually do not provide the same diversity as seagrass meadows. High biodiversity is essential for an ecosystem’s stability and our lives: healthy coastal ecosystems provide food and shelter for fish species and stabilize the seafloor. We investigated a relatively unknown type of red macroalgae and were surprised to find it thriving with marine organisms such as sea stars, anemones, and tube worms. With the latter being an example of an extraordinary group of marine animals, we would like to take this example and show you what we learned about this new hotspot for diversity.
Operational solar flare forecasting via video-based deep learning
Authors: Guastavino Sabrina , Marchetti Francesco , Benvenuto Federico , Campi Cristina , Piana Michele
Published: March 2023

Operational flare forecasting aims at providing predictions that can be used to make decisions, typically on a daily scale, about the space weather impacts of flare occurrence. This study shows that video-based deep learning can be used for operational purposes when the training and validation sets used for network optimization are generated while accounting for the periodicity of the solar cycle. Specifically, this article describes an algorithm that can be applied to build up sets of active regions that are balanced according to the flare class rates associated to a specific cycle phase. These sets are used to train and validate a long-term recurrent convolutional network made of a combination of a convolutional neural network and a long short-term memory network. The reliability of this approach is assessed in the case of two prediction windows containing the solar storms of March 2015, June 2015, and September 2017.
Changes in membrane fatty acids of a halo-psychrophile exposed to magnesium perchlorate and low temperatures: Implications for Mars habitability
Authors: García-Descalzo Laura , Lezcano María Ángeles , Carrizo Daniel , Fairén Alberto G.
Published: March 2023

The presence of perchlorate salts in aqueous solutions bears two opposite effects on habitability. On the one hand, perchlorate salts trigger a decrease in the freezing point of the aqueous solutions, resulting in stable aqueous solutions at subzero temperatures, thereby widening the habitable conditions for potential microbial life. On the other hand, the presence of perchlorates in solution imposes a significant osmotic stress that compromises the integrity of microbial cell membranes, thereby restricting the habitable conditions in the same aqueous environment. Here we investigated the survivability and the changes in the composition of membrane fatty acids (FAs) of the bacterium Rhodococcus sp. JG-3 cells under warm (20°C), cold (4°C), and subzero temperatures (−10°C and −16°C), and in the presence (8 wt% and 16 wt%) and absence of magnesium perchlorate (Mg(ClO4)2). Bacterial cell survivability decreased with decreasing temperature and presence of magnesium perchlorate. However, Rhodococcus sp. JG-3 was able to tolerate up to 8 wt% Mg(ClO4)2 at −16°C. The presence of magnesium perchlorate in the medium decreased the concentration of total FAs, likely due to a destabilization of the molecules by the chaotropic effect of the perchlorate anion. At the maximum stress (both subzero temperatures and 16 wt% magnesium perchlorate), the composition of FAs changed, i.e., Rhodococcus sp. JG-3 cells increased the relative abundance of saturated FAs (SFAs) over the unsaturated (UFAs) or branched (BFAs). These changes in the proportion of FAs types may be a physiological response during cooling, aimed to improve lipid membrane stability. Interestingly, the composition and relative abundance of fatty acid types (i.e., SFAs, UFAs and BFAs) of Rhodococcus sp. JG-3 when simultaneously exposed to subzero temperatures and 16 wt% magnesium perchlorate was similar to that following freezing stress alone, suggesting that either both conditions triggered a similar response or that one response dominated over the other. Our findings contribute to understand the survivability and adaptation of extremophilic microorganisms under polyextreme conditions, such as those existing in the Martian subsurface today and/or in the past, which include the documented presence of magnesium perchlorate salts in ancient sediments and global cold temperatures.
Improved space weather observations and modeling for aviation radiation
Authors: Bain H. M. , Onsager T. G. , Mertens C. J. , Copeland K. , Benton E. R. , Clem J. , Mangeard P.-S. , Green J. C. , Guild T. B. , Tobiska W. K. , Shelton-Mur K. , Zheng Y. , Halford A. J. , Carlson S. , Pulkkinen A.
Published: March 2023

In recent years there has been a growing interest from the aviation community for space weather radiation forecasts tailored to the needs of the aviation industry. In 2019 several space weather centers began issuing advisories for the International Civil Aviation Organization alerting users to enhancements in the radiation environment at aviation flight levels. Due to a lack of routine observations, radiation modeling is required to specify the dose rates experienced by flight crew and passengers. While mature models exist, support for key observational inputs and further modeling advancements are needed. Observational inputs required from the ground-based neutron monitor network must be financially supported for research studies and operations to ensure real-time data is available for forecast operations and actionable end user decision making. An improved understanding of the geomagnetic field is required to reduce dose rate uncertainties in regions close to the open/closed geomagnetic field boundary, important for flights such as those between the continental US and Europe which operate in this region. Airborne radiation measurements, which are crucial for model validation and improvement, are lacking, particularly during solar energetic particle events. New measurement campaigns must be carried out to ensure progress and in situ atmospheric radiation measurements made available for real-time situational awareness. Furthermore, solar energetic particle forecasting must be improved to move aviation radiation nowcasts to forecasts in order to meet customer requirements for longer lead times for planning and mitigation.
New improved incision-tubing approach for bronchoesophageal Fistula with mediastinal abscess after esophagectomy: A case report
Authors: Cao Jianwei , Geng Mingfei , Huang Xiaoyu , Liu Changjiang , Li Hui
Published: March 2023

Bronchoesophageal fistula is a serious threat to the survival after esophagectomy for esophageal cancer. The erosion of mediastinal abscess post anastomotic leakage is the most likely directly cause. However, the bronchoesophageal fistula with gastric conduit necrosis and mediastinal abscess is refractory to either surgical or conservative treatment. In the article, a unique case of Bronchoesophageal fistula with mediastinal abscess after gastric conduit necrosis is presented. A 74-year-old female was detected the right inferior bronchus-esophageal fistula with mediastinal abscess on 15 postoperative day after esophagectomy for esophageal cancer. A successful new improved minimally invasive management was performed.
The rPRRSV-E2 strain exhibited a low level of potential risk for virulence reversion
Authors: Jiang Yifeng , Gao Fei , Li Liwei , Zhou Yanjun , Tong Wu , Yu Lingxue , Zhang Yujiao , Zhao Kuan , Zhu Haojie , Liu Changlong , Li Guoxin , Tong Guangzhi
Published: March 2023

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Classical Swine Fever Virus (CSFV) are two important pathogens, which cause serious impact on swine industry worldwide. In our previous research, rPRRSV-E2, the recombinant PRRSV expressing CSFV E2 protein, could provide sufficient protection against the lethal challenge of highly pathogenic PRRSV and CSFV, and could maintained genetically stable in vitro. Here, to evaluate the virulence reversion potential risk, rPRRSV-E2 had been continuously passaged in vivo, the stability of E2 expression and virulence of the passage viruses were analyzed. The results showed that no clinical symptoms or pathological changes could be found in the inoculated groups, and there were no significant differences of viraemia among the test groups. Sequencing and IFA analysis showed that the coding gene of exogenous CSFV E2 protein existed in the passaged viruses without any sequence mutations, deletions or insertions, and could expressed steadily. It could be concluded that the foreign CSFV E2 gene in the genome of rPRRSV-E2 could be maintained genetically stable in vivo, and rPRRSV-E2 strain had relatively low level of potential risk for virulence reversion.
Identification of surfactin as an anti-severe fever with thrombocytopenia syndrome virus multi-target compound extracted from the culture broth of marine microbes
Authors: Urata Shuzo , Takouda Jun , Watanabe Yoshihiro , Sakaguchi Miako , Sakurai Yasuteru , Inahashi Yuki , Iwatsuki Masato , Yasuda Jiro , Tanaka Yoshimasa , Takeda Kohsuke
Published: March 2023

Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne virus first identified in China in 2011 and later reported in other Asian countries. Significant efforts have been made to develop anti-SFTSV compounds; however, there are no approved vaccines or antivirals against SFTSV infections. Marine organisms provide nearly unlimited biological resources to produce therapeutic drugs for the treatment and control of disease. In this study, we aimed to identify anti-SFTSV chemical compounds from the culture broth extracts of marine microbes collected from the coasts of the Nagasaki Prefecture, Japan. Of the 80 extracts, two showed an anti-SFTSV effect. One of them, which exhibited low cell toxicity, was used for further characterization. Chemical analysis combined with the anti-SFTSV effect identified surfactin as one of the main components of the selected extract. Our study showed a proof-of-concept to identify novel antiviral compounds from marine microbes against the virus of interest. Further analysis showed that surfactin affected the integrity of the virion membrane and inhibited SFTSV infection-induced membrane fusion at low pH conditions. Furthermore, surfactin inhibits the post-entry step of viral replication in the cell, which is a novel mode of antiviral action of surfactin. These results indicate that surfactin can target multiple steps of SFTSV replication in cells.
Agricultural Use of Pesticides in Galicia (NW Spain) During the 20th Century: Inventory of Active Substances and Their Geographic Distribution
Authors: Chaves Raquel , Monterroso Carmela
Published: March 2023

Production of agricultural pesticides underwent an exponential increase during the 20th century. Many of these pesticides have since been prohibited because they are toxic and because they are bioaccumulated and persist in the environment. Detailed knowledge about any substance that is applied to land is essential to enable evaluation of potential contamination effects and the risk of latent exposure to these types of substances. However, accurate determination of the type and amounts of pesticides used throughout the history of agriculture in Spain is greatly complicated by the absence of historical records, scarce control of use and the lack of effective legislation until well into the 21st century. The aims of this study were: 1) to elaborate an inventory of the active substances of the agricultural pesticides (phytosanitary products) used in Galicia throughout the 20th century, 2) to develop a model of their geographic distribution by using a geographic information system (GIS), and 3) to contrast the geographical model obtained for selected persistent pesticides with the presence of these compounds in raw water. We consulted a large variety of sources of information and carried out a geographic analysis for the most commonly used pesticides in different crops. We inventoried a total of 176 active substances belonging to more than 50 different classes of chemicals and observed a clear geographic zoning according to the chemical class and mode of action of the substances. The presence of pesticide residues at drinking water extraction points is consistent with the geographic delimitation established in the study.
Nearest Neighbours Graph Variational AutoEncoder
Authors: Arsini Lorenzo , Caccia Barbara , Ciardiello Andrea , Giagu Stefano , Mancini Terracciano Carlo
Published: March 2023

Graphs are versatile structures for the representation of many real-world data. Deep Learning on graphs is currently able to solve a wide range of problems with excellent results. However, both the generation of graphs and the handling of large graphs still remain open challenges. This work aims to introduce techniques for generating large graphs and test the approach on a complex problem such as the calculation of dose distribution in oncological radiotherapy applications. To this end, we introduced a pooling technique (ReNN-Pool) capable of sampling nodes that are spatially uniform without computational requirements in both model training and inference. By construction, the ReNN-Pool also allows the definition of a symmetric un-pooling operation to recover the original dimensionality of the graphs. We also present a Variational AutoEncoder (VAE) for generating graphs, based on the defined pooling and un-pooling operations, which employs convolutional graph layers in both encoding and decoding phases. The performance of the model was tested on both the realistic use case of a cylindrical graph dataset for a radiotherapy application and the standard benchmark dataset sprite. Compared to other graph pooling techniques, ReNN-Pool proved to improve both performance and computational requirements.
Sustainable Sound Absorbers to Improve Acoustical Comfort in Atria: A Methodological Approach
Authors: Rubino Chiara , Liuzzi Stefania , Martellotta Francesco
Published: March 2023

Resource-efficient buildings are one of the most important challenges of the construction industry, which could be achieved by limiting the extraction of natural resources and by replacing them with industrial residues. The present work proposes innovative panels made from textile wastes as an efficient solution for making the semi-open atrium in the Polytechnic University of Bari, acoustically suitable for use as an area of study. Several measurements were carried out in the atrium under empty and occupied conditions in order to characterize the sound field of the space (i.e., the reverberation time and the sound pressure level) and the actual sound sources (i.e., the sound power level of the students occupying the space). The on-site measurement results were useful to calibrate a geometrical acoustic model implemented in CATT-Acoustic software and used to predict the effect of the proposed treatments. About 700 m2 of absorbent panels were distributed, leading to a predicted reduction in the reverberation time from 2.4 s to 1.4 s at 500 Hz, and consequently to an estimated 3 dB reduction in sound pressure level, resulting in a reduction in the background noise level due to speech. Furthermore, as a consequence of the “Lombard effect”, a decrease in the background noise level will likely reduce the sound power level of the speakers by 2 dB, with an expected overall reduction of 5 dB.