bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of an IGF2BP2-Targeted Peptide for Near-Infrared Imaging of Esophageal Squamous Cell Carcinoma
Authors: Shu Wenbin , Xiao Yitai , Wang Lizhu , Liang Mingzhu , Li Zhihong , Wu Xiangwen , Cao Qingdong
Published: November 2022

Esophageal squamous cell carcinoma (ESCC) is one of the most lethal malignancies globally. Peptide-based tumor-targeted imaging is critical for ESCC imaging. In this study, we aim to identify a peptide-targeting IGF2BP2 that specifically binds to human ESCC for near-infrared imaging of esophageal cancer. Applying phage display techniques, we identified a peptide target for IGF2BP2 which was confirmed to be highly expressed in ESCC cell lines or tumor tissue and may serve as an imaging target for ESCC. We conjugated the peptide to the NIRF group, Cy5, and further evaluated the targeting efficacy of the probe at a cellular level and in animal tumor models. The Cy5 conjugated peptide (P12-Cy5) showed a high binding affinity to human ESCC cells in vitro. In vivo, optical imaging also validated the tumor-targeting ability of P12-Cy5 in KYSE-30-bearing subcutaneous ESCC tumor models. Furthermore, the results of biodistribution showed a significantly higher fluorescence intensity in tumors compared to scrambled peptide, which is consistent with in vivo observations. In summary, an IGF2BP2-targeted peptide was successfully identified. In vitro and in vivo experiments confirmed that P12-Cy5 has high affinity, specificity and tumor-targeting properties. Thus, P12-Cy5 is a prospective NIR probe for the imaging of ESCC.
New Non-Fullerene Acceptor with Extended Conjugation of Cyclopenta [2,1-b:3,4-b’] Dithiophene for Organic Solar Cells
Authors: Sun Cheng , Lee Sanseong , Choi Changeun , Jeong Soyeong , Oh Juhui , Kim Ju-Hyeon , Kim Jaeyoung , Baek Ho Eon , Kang Hongkyu , Jang Soo-Young , Choi Hyun Ho , Lee Kwanghee , Kim Yun-Hi
Published: November 2022

Herein, we design and characterize 9-heterocyclic ring non-fullerene acceptors (NFAs) with the extended backbone of indacenodithiophene by cyclopenta [2,1-b:3,4-b’] dithiophene (CPDT). The planar conjugated CPDT donor enhances absorption by reducing vibronic transition and charge transport. Developed NFAs with different end groups shows maximum absorption at approximately 790–850 nm in film. Because of the electronegative nature of the end-group, the corresponding acceptors showed deeper LUMO energy levels and red-shifted ultraviolet absorption. We investigate the crystallinity, film morphology, surface energy, and electronic as well as photovoltaic performance. The organic photovoltaic cells using novel NFAs with the halogen end groups fluorine or chlorine demonstrate better charge collection and faster exciton dissociation than photovoltaic cells using NFAs with methyl or lacking a substituent. Photovoltaic devices constructed from m-Me-ITIC with various end groups deliver power conversion efficiencies of 3.6–11.8%.
Amorphous Co-Mo-B Film: A High-Active Electrocatalyst for Hydrogen Generation in Alkaline Seawater
Authors: Fang Xiaodong , Wang Xiangguo , Ouyang Ling , Zhang Longcheng , Sun Shengjun , Liang Yimei , Luo Yongsong , Zheng Dongdong , Kang Tairan , Liu Qian , Huo Feng , Sun Xuping
Published: November 2022

The development of efficient electrochemical seawater splitting catalysts for large-scale hydrogen production is of great importance. In this work, we report an amorphous Co-Mo-B film on Ni foam (Co-Mo-B/NF) via a facile one-step electrodeposition process. Such amorphous Co-Mo-B/NF possesses superior activity with a small overpotential of 199 mV at 100 mA cm−2 for a hydrogen evolution reaction in alkaline seawater. Notably, Co-Mo-B/NF also maintains excellent stability for at least 24 h under alkaline seawater electrolysis.
Assessment of Cucurbita spp. Peel Extracts as Potential Sources of Active Substances for Skin Care and Dermatology
Authors: Gaweł-Bęben Katarzyna , Czech Karolina , Strzępek-Gomółka Marcelina , Czop Marcin , Szczepanik Monika , Lichtarska Anna , Kukula-Koch Wirginia
Published: November 2022

By-products of cultivated plants are one of the major environmental concerns worldwide. Due to the high concentration of bioactive chemicals, such waste may be considered hazardous due to the interference with the plant growth, deterioration of the drinking water quality or toxic effects on sensitive marine organisms. Moreover, plant-derived by-products, with proper handling, may represent a low-cost source of bioactive compounds potentially important for pharmaceutical and cosmetics industries. The aim of the study was to evaluate the phytochemical composition, antioxidant activity, the influence of tyrosinase activity, in vitro sun protecting factor and cytotoxicity of 15 extracts from peels of five cultivars of Cucurbita maxima and C. moschata. The extracts were prepared using “green solvents” (water, 50% propylene glycol, and 20% ethanol) and ultrasound-assisted extraction. The performed analysis showed that the peel extracts from various cultivars differ significantly in respect to the phytochemical content and activity. The type of solvent also had a significant impact on the extract’s composition and bioactivity. Aqueous peel extracts contained the highest amounts of flavonoids, showed the greatest antioxidant potential and the most significant in vitro SPF values. In vitro studies showed that the analyzed peel extracts are not cytotoxic for human keratinocytes up to the concentration of 1000 µg/mL and thus might be considered as non-irritant for the skin. The study confirms the potential application of peel extracts from Cucurbita spp. cultivars in cosmetic products.
Influence of Group-IVA Doping on Electronic and Optical Properties of ZnS Monolayer: A First-Principles Study
Authors: Liu Bin , Su Wan-Sheng , Wu Bi-Ru
Published: November 2022

Element doping is a universal way to improve the electronic and optical properties of two-dimensional (2D) materials. Here, we investigate the influence of group−ⅣA element (C, Si, Ge, Sn, and Pb) doping on the electronic and optical properties of the ZnS monolayer with a tetragonal phase by using first-principles calculations. The results indicate that the doping atoms tend to form tetrahedral structures with neighboring S atoms. In these doped models, the formation energies are all negative, indicating that the formation processes of the doped models will release energy. The formation energy is smallest for C−doped ZnS and gradually increases with the metallicity of the doping element. The doped ZnS monolayer retains a direct band gap, with this band gap changing little in other element doping cases. Moreover, intermediate states are observed that are induced by the sp3 hybridization from the doping atoms and S atoms. Such intermediate states expand the optical absorption range into the visible spectrum. Our findings provide an in-depth understanding of the electronic and optical properties of the ZnS monolayer and the associated doping structures, which is helpful for application in optoelectronic devices.
The Mechanism of the Photostability Enhancement of Thin-Film Transistors Based on Solution-Processed Oxide Semiconductors Doped with Tetravalent Lanthanides
Authors: Lan Linfeng , Ding Chunchun , He Penghui , Su Huimin , Huang Bo , Xu Jintao , Zhang Shuguang , Peng Junbiao
Published: November 2022

The applications of thin-film transistors (TFTs) based on oxide semiconductors are limited due to instability under negative bias illumination stress (NBIS). Here, we report TFTs based on solution-processed In2O3 semiconductors doped with Pr4+ or Tb4+, which can effectively improve the NBIS stability. The differences between the Pr4+-doped In2O3 (Pr:In2O3) and Tb4+-doped In2O3 (Tb:In2O3) are investigated in detail. The undoped In2O3 TFTs with different annealing temperatures exhibit poor NBIS stability with serious turn-on voltage shift (ΔVon). After doping with Pr4+/Tb4+, the TFTs show greatly improved NBIS stability. As the annealing temperature increases, the Pr:In2O3 TFTs have poorer NBIS stability (ΔVon are −3.2, −4.8, and −4.8 V for annealing temperature of 300, 350, and 400 °C, respectively), while the Tb:In2O3 TFTs have better NBIS stability (ΔVon are −3.6, −3.6, and −1.2 V for annealing temperature of 300, 350, and 400 ℃, respectively). Further studies reveal that the improvement of the NBIS stability of the Pr4+/Tb4+:In2O3 TFTs is attributed to the absorption of the illuminated light by the Pr/Tb4fn—O2p6 to Pr/Tb 4fn+1—O2p5 charge transfer (CT) transition and downconversion of the light to nonradiative transition with a relatively short relaxation time compared to the ionization process of the oxygen vacancies. The higher NBIS stability of Tb:In2O3 TFTs compared to Pr:In2O3 TFTs is ascribed to the smaller ion radius of Tb4+ and the lower energy level of Tb 4f7 with a isotropic half-full configuration compared to that of Pr 4f1, which would make it easier for the Tb4+ to absorb the visible light than the Pr4+.
Electrospun Poly(acrylic acid-co-4-styrene sulfonate) as Potential Drug-Eluting Scaffolds for Targeted Chemotherapeutic Delivery Systems on Gastric (AGS) and Breast (MDA-Mb-231) Cancer Cell Lines
Authors: Neira-Carrillo Andrónico , Zárate Ignacio A. , Nieto Eddie , Butto-Miranda Nicole , Lobos-González Lorena , Del Campo-Smith Matias , Palacio Daniel A. , Urbano Bruno F.
Published: November 2022

Potential drug-eluting scaffolds of electrospun poly(acrylic acid-co-styrene sulfonate) P(AA-co-SS) in clonogenic assays using tumorigenic gastric and ovarian cancer cells were tested in vitro. Electrospun polymer nanofiber (EPnF) meshes of PAA and PSSNa homo- and P(AA-co-SS) copolymer composed of 30:70, 50:50, 70:30 acrylic acid (AA) and sodium 4-styrene sulfonate (SSNa) units were performed by electrospinning (ES). The synthesis, structural and morphological characterization of all EPnF meshes were analyzed by optical and electron microscopy (SEM-EDS), infrared spectroscopy (FTIR), contact angle, and X-ray diffraction (XRD) measurements. This study shows that different ratio of AA and SSNa of monomers in P(AA-co-SS) EPnF play a crucial role in clonogenic in vitro assays. We found that 50:50 P(AA-co-SS) EPnF mesh loaded with antineoplastic drugs can be an excellent suppressor of growth-independent anchored capacities in vitro assays and a good subcutaneous drug delivery system for chemotherapeutic medication in vivo model for surgical resection procedures in cancer research.
Accurate and Comprehensive Spectrum Characterization for Cavity-Enhanced Electro-Optic Comb Generators
Authors: Yang Ruitao , Wu Jinxuan , Yang Hongxing , Fu Haijin , Yu Liang , Xing Xu , Dong Yisi , Hu Pengcheng , Tan Jiubin
Published: November 2022

Cavity-enhanced electro-optic comb generators (CEEOCGs) can provide optical frequency combs with excellent stability and configurability. The existing methods for CEEOCGs spectrum characterization, however, are based on approximations and have suffered from either iterative calculations or limited applicable conditions. In this paper, we show a spectrum characterization method by accumulating the optical electrical field with respect to the count of the round-trip propagation inside of CEEOCGs. The identity transformation and complete analysis of the intracavity phase delay were conducted to eliminate approximations and be applicable to arbitrary conditions, respectively. The calculation efficiency was improved by the noniterative matrix operations. Setting the maximum propagation count as 1000, the spectrum of the center ±300 comb modes can be characterized with merely the truncation error of floating-point numbers within 1.2 s. More importantly, the effects of all CEEOCG parameters were comprehensively characterized for the first time. Accordingly, not only the exact working condition of CEEOCG can be identified for further optimization, but also the power of each comb mode can be predicted accurately and efficiently for applications in optical communications and waveform synthesis.
Diverse Pathways of Engineered Nanoparticle-Induced NLRP3 Inflammasome Activation
Authors: Liao Xin , Liu Yudong , Zheng Jiarong , Zhao Xinyuan , Cui Li , Hu Shen , Xia Tian , Si Shanshan
Published: November 2022

With the rapid development of engineered nanomaterials (ENMs) in biomedical applications, their biocompatibility and cytotoxicity need to be evaluated properly. Recently, it has been demonstrated that inflammasome activation may be a vital contributing factor for the development of biological responses induced by ENMs. Among the inflammasome family, NLRP3 inflammasome has received the most attention because it directly interacts with ENMs to cause the inflammatory effects. However, the pathways that link ENMs to NLRP3 inflammasome have not been thoroughly summarized. Thus, we reviewed recent findings on the role of major ENMs properties in modulating NLRP3 inflammasome activation, both in vitro and in vivo, to provide a better understanding of the underlying mechanisms. In addition, the interactions between ENMs and NLRP3 inflammasome activation are summarized, which may advance our understanding of safer designs of nanomaterials and ENM-induced adverse health effects.
Fe3O4-Nanoparticle-Doped Epoxy Resin as a Detachable Adhesive by Electromagnetic Heating for GFRP Single-Lap Joints
Authors: Sánchez-Romate Xoan , del Bosque Antonio , Crespo Anabel , Alonso Rafael , Sánchez María , Ureña Alejandro
Published: November 2022

An adhesive based on a Fe3O4-nanoparticle (MNP)-doped epoxy resin was proposed for the development of detachable adhesive joints with GFRP substrates. The analysis of cryofractures showed that the increasing MNP content promotes a higher presence of larger aggregates and a lower sedimentation of nanoparticles due to the higher viscosity of the mixture. In this regard, the inclusion of expandable microspheres (MS) induces a more uniform dispersion of MNPs, reducing their sedimentation. The capability of the proposed adhesives for electromagnetic (EM) heating was also evaluated, with increases in temperature of around 100 °C at 750 A, enough to reach the Tg of the polymer required to facilitate the adhesive detachment, which is around 80 °C. Finally, the lap shear strength (LSS) of 14 and 20 wt.% MNP samples was evaluated in a single-lap shear joint with simultaneous EM heating. The LSS values were reduced by 60–80% at 750 A, thus promoting successful adhesive joint detachment under EM heating.
The Role of Catechins in Regulating Diabetes: An Update Review
Authors: Wen Lianghua , Wu Dan , Tan Xindong , Zhong Meiqi , Xing Jiabao , Li Wei , Li Dan , Cao Fanrong
Published: November 2022

Catechins are key functional components in tea and have many health benefits, including relieving diabetes. Glucose is necessary for maintaining life. However, when the glucose in the serum exceeds the threshold, it will lead to hyperglycemia. Hyperglycemia is mainly caused by insufficient insulin secretion or insulin resistance. Persistent hyperglycemia can cause various disorders, including retinopathy, nephropathy, neurodegenerative diseases, cardiovascular disease, and diabetes. In this paper, we summarize the research on the underlying mechanisms of catechins in regulating diabetes and elaborate on the mechanisms of catechins in alleviating hyperglycemia by improving insulin resistance, alleviating oxidative stress, regulating mitochondrial function, alleviating endoplasmic reticulum stress, producing anti-inflammatory effects, reducing blood sugar source, and regulating intestinal function. This review will provide scientific direction for future research on catechin alleviating diabetes.
Exploration of the Gut–Brain Axis through Metabolomics Identifies Serum Propionic Acid Associated with Higher Cognitive Decline in Older Persons
Authors: Neuffer Jeanne , González-Domínguez Raúl , Lefèvre-Arbogast Sophie , Low Dorrain Y. , Driollet Bénédicte , Helmer Catherine , Du Preez Andrea , de Lucia Chiara , Ruigrok Silvie R. , Altendorfer Barbara , Aigner Ludwig , Lucassen Paul J. , Korosi Aniko , Thuret Sandrine , Manach Claudine , Pallàs Mercè , Urpi-Sardà Mireia , Sánchez-Pla Alex , Andres-Lacueva Cristina , Samieri Cécilia
Published: November 2022

The gut microbiome is involved in nutrient metabolism and produces metabolites that, via the gut–brain axis, signal to the brain and influence cognition. Human studies have so far had limited success in identifying early metabolic alterations linked to cognitive aging, likely due to limitations in metabolite coverage or follow-ups. Older persons from the Three-City population-based cohort who had not been diagnosed with dementia at the time of blood sampling were included, and repeated measures of cognition over 12 subsequent years were collected. Using a targeted metabolomics platform, we identified 72 circulating gut-derived metabolites in a case–control study on cognitive decline, nested within the cohort (discovery n = 418; validation n = 420). Higher serum levels of propionic acid, a short-chain fatty acid, were associated with increased odds of cognitive decline (OR for 1 SD = 1.40 (95% CI 1.11, 1.75) for discovery and 1.26 (1.02, 1.55) for validation). Additional analyses suggested mediation by hypercholesterolemia and diabetes. Propionic acid strongly correlated with blood glucose (r = 0.79) and with intakes of meat and cheese (r > 0.15), but not fiber (r = 0.04), suggesting a minor role of prebiotic foods per se, but a possible link to processed foods, in which propionic acid is a common preservative. The adverse impact of propionic acid on metabolism and cognition deserves further investigation.
A Town-Level Comprehensive Intervention Study to Reduce Salt Intake in China: Cluster Randomized Controlled Trial
Authors: Liu Min , Xu Jianwei , Li Yuan , He Feng J , Zhang Puhong , Song Jing , Gao Yifu , Yan Shichun , Yan Wei , Jin Donghui , Chang Xiaoyu , Xu Zhihua , Bai Yamin , Ji Ning , Wu Jing
Published: November 2022

We determined whether a town-level comprehensive intervention program could lower the salt intake of a population. The parallel, cluster randomized controlled trial was carried out between October 2018 and January 2020 in 48 towns from 12 counties across 6 provinces in China. All participants were asked to complete the 24 h urine collections, anthropometric measurements and questionnaires at the baseline and one-year post-intervention survey. A total of 2693 participants aged 18 to 75 years were recruited at the baseline. A total of 1347 individuals in 24 towns were allocated to the intervention group and the others were allocated to the control group. Valid information from 2335 respondents was collected in the follow-up survey. The 24-h urinary sodium excretion was 189.7 mmol/24 h for the intervention group and 196.1 mmol/24 h for the control group at baseline. At a one-year follow-up, the mean effect of salt intake did not show a significant change (p = 0.31) in the intervention group compared to the control group. However, the mean result of potassium excretion in the intervention group increased by 2.18 mmol/24 h (85.03 mg/24 h) (p = 0.004) and systolic blood pressure decreased by 2.95 mmHg (p < 0.001). The salt-related knowledge and attitude toward salt reduction improved significantly in the intervention group (p < 0.05). A longer period of intervention and follow-up assessment might be needed to evaluate the long-term effectiveness of the program on salt reduction.
Association of Gut Microbiota Composition in Pregnant Women Colonized with Group B Streptococcus with Maternal Blood Routine and Neonatal Blood-Gas Analysis
Authors: Wang Zhixia , Pu Wenyuan , Liu Qi , Zhu Meifeng , Chen Qinlei , Xu Yingchun , Zhou Chunxiang
Published: November 2022

Group B Streptococcus (GBS) colonizes the vaginal and rectal mucosa in a substantial proportion of healthy women, and GBS is a risk factor for GBS-associated adverse birth outcomes, such as bacterial infection, in neonates. Whether changes in the gut microbiota of GBS-infected pregnant women are associated with maternal complete blood cell count (CBC) and neonatal blood-gas analysis is unknown. To explore the relationship between the intestinal microecological composition of pregnant women and maternal blood routine and neonatal blood-gas analysis, we collected intestinal microecology samples of 26 pregnant women in clinic. They were divided into a positive group(GBS positive,GBS +) and a negative group (GBS negative, GBS-), with 12 in the positive group and 14 in the negative group. 16S rRNA gene sequencing was used to examine the gut microbiota profile from a fecal sample of pregnant women. CBC was carried out in enrolled pregnant women and umbilical arterial blood-gas analysis (UABGA)was conducted for analysis of intestinal microbiota composition, maternal blood routine and neonatal blood gas. Our results showed significant differences in the total number of organisms and microbial diversity of intestinal microbiota between healthy pregnant women and GBS-positive pregnant women. Particularly, abundances of Lentisphaerae, Chlorobi, Parcubacteria, Chloroflexi, Gemmatimonadetes, Acidobacteria, Fusobacteria and Fibrobacteres were only detected in participants with GBS colonization. Blood-gas analysis revealed that neonates born to mothers with GBS colonization had significantly higher fractions of carboxyhemoglobin (FCOHb) and lower methemoglobin (FMetHb), and abundances of OTU80, OTU122, OTU518 and OTU375 were associated with blood-gas indicators, such as carboxyhemoglobin, methemoglobin, PCO2, PH and ABE. Interestingly, there were significant correlations between OTU levels and inflammatory indexes in pregnant women with GBS infection. Together, this study revealed for the first time that altered gut microbiota compositions are related to the inflammatory state in GBS-positive pregnant women and neonatal blood-gas indicators. GBS colonization may lead to significant changes in the gut microbiome, which might be involved in the pathogenesis of the maternal inflammatory state and neonatal blood gas abnormalities.
Effects of Caffeine, a DNA Damage Response Inhibitor, on Papillomavirus Genome Replication
Authors: Kanginakudru Sriramana , Gilson Timra , Jose Leny , Androphy Elliot J.
Published: November 2022

Epidemiological studies have revealed that caffeinated coffee imparts a reduced risk of oropharyngeal cancer, of which human papillomavirus (HPV) is one of the causative agents. Caffeine is a known inhibitor of the DNA damage response (DDR) pathway. We sought to test the effects of caffeine on the early replication of the HPV31 virus. It has been reported that the inhibition of several factors necessary for the DDR during the differentiation-dependent stage of HPV block genome amplification, while the HPV genome maintenance replication was unaffected. We first studied the effects of caffeine in the earliest stages of viral infection. Using pseudo-virions (PsV) expressing an m-Cherry reporter gene and quasi-virions (QsV) containing HPV31 genomes to mediate the infection, we found no evidence that caffeine impeded the viral entry; however, the infected cells displayed a reduced HPV copy number. In contrast, caffeine exposure increased the copy number of HPV31 episomes in the transient transfection assays and in the CIN612E cells that stably maintain viral episomes. There was a concomitant increase in the steady state levels of the HPV31 E1 and E2 transcripts, along with increased E2 loading at the viral origin of replication (ori). These results suggest that the caffeine-mediated inhibition of the DDR reduces viral genome replication in the early stage of infection, in contrast to the maintenance stage, in which the inhibition of the DDR may lead to an increase in viral amplicon replication.
Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae)
Authors: Vicente Roberto Dezan , Madeira Fernanda Fernandez , Borsatto Kelly Cristine , Garcia Ariane Cristina Caris , Cristal Daniel Cesaretto , Delgado Luiza Maria Grzyb , Bittinelli Isadora de Freitas , De Mello Denis Vinicius , Dos Reis Yago Visinho , Ravazi Amanda , Galvão Cleber , De Azeredo-Oliveira Maria Tercília Vilela , Da Rosa João Aristeu , De Oliveira Jader , Alevi Kaio Cesar Chaboli
Published: November 2022

Under laboratory conditions, Triatoma rosai and T. sordida are able to cross and produce hybrids. In the face of climate and environmental changes, the study of hybrids of triatomines has evolutionary and epidemiological implications. Therefore, we performed morphological, cytological and molecular studies and characterized the feeding and defecation pattern of hybrids from crosses between T. sordida and T. rosai. The morphological characterization of the female genitalia of the hybrids showed that characteristics of both parental species segregated in the hybrids. Cytogenetic analyzes of hybrids showed regular metaphases. According to molecular studies, the mitochondrial marker Cytochrome B (CytB) related the hybrids with T. sordida and the nuclear marker Internal Transcribed Spacer 1 (ITS-1) related the hybrids with T. rosai. Both parents and hybrids defecated during the blood meal. Thus, the hybrids resulting from the cross between T. sordida and T. rosai presented segregation of phenotypic characters of both parental species, 100% homeology between homeologous chromosomes, phylogenetic relationship with T sordida and with T. rosai (with CytB and ITS-1, respectively), and, finally, feeding and defecation patterns similar to the parents.
Potential Hybridization of Fasciola hepatica and F. gigantica in Africa—A Scoping Review
Authors: Nukeri Sophy , Malatji Mokgadi Pulane , Sengupta Mita Eva , Vennervald Birgitte Jyding , Stensgaard Anna-Sofie , Chaisi Mamohale , Mukaratirwa Samson
Published: November 2022

The occurrence of Fasciola gigantica and F. hepatica in Africa is well documented; however, unlike in Asia, there is a paucity of information on the existence of hybrids or parthenogenetic species on the continent. Nonetheless, these hybrid species may have beneficial characteristics, such as increased host range and pathogenicity. This study provides evidence of the potential existence of Fasciola hybrids in Africa. A literature search of articles published between 1980 and 2022 was conducted in PubMed, Google Scholar, and Science Direct using a combination of search terms and Boolean operators. Fasciola species were documented in 26 African countries with F. hepatica being restricted to 12 countries, whilst F. gigantica occurred in 24 countries, identified based on morphological features of adult Fasciola specimens or eggs and molecular techniques. The co-occurrence of both species was reported in 11 countries. However, the occurrence of potential Fasciola hybrids was only confirmed in Egypt and Chad but is suspected in South Africa and Zimbabwe. These were identified based on liver fluke morphometrics, assessment of the sperms in the seminal vesicle, and molecular techniques. The occurrence of intermediate host snails Galba truncatula and Radix natalensis was reported in Ethiopia, Egypt, South Africa, Tanzania, and Uganda, where F. hepatica and F. gigantica co-occurrences were reported. The invasive Pseudosuccinea columella snails naturally infected with F. gigantica were documented in South Africa and Egypt. In Zimbabwe, P. columella was infected with a presumed parthenogenetic Fasciola. This suggests that the invasive species might also be contributing to the overlapping distributions of the two Fasciola species since it can transmit both species. Notwithstanding the limited studies in Africa, the potential existence of Fasciola hybrids in Africa is real and might mimic scenarios in Asia, where parthenogenetic Fasciola exist in most Asian countries. In South Africa, aspermic F. hepatica and Fasciola sp. have been reported already, and Fasciola hybrids have been reported? in Chad and Egypt. Thus, the authors recommend future surveys using molecular markers recommended to identify Fasciola spp. and their snail intermediate hosts to demarcate areas of overlapping distribution where Fasciola hybrids and/or parthenogenetic Fasciola may occur. Further studies should also be conducted to determine the presence and role of P. columella in the transmission of Fasciola spp. in these geographical overlaps to help prevent parasite spillbacks.
The Paradoxical Effects of Serum Amyloid-P Component on Disseminated Candidiasis
Authors: Klotz Stephen A. , Lipke Peter N.
Published: November 2022

Serum amyloid P component (SAP) may play an important role in human fungal diseases. SAP binds to functional amyloid on the fungal surface and masks fungi from host immune processes, skewing the macrophage population from the pro-inflammatory M1 to the quiescent M2 type. We assessed the role of SAP in a murine model of disseminated candidiasis. Mice were injected with human SAP subcutaneously (SQ) followed by intravenous injection of Candida albicans. Male, BALBcJ mice were administered 2 mg human SAP or the homologous human pro-inflammatory pentraxin CRP, SQ on day −1 followed by 1 mg on days 0 thru 4; yeast cells were administered intravenously on day 0. Mice not receiving a pentraxin were morbid on day 1, surviving 4–7 days. Mice administered SAP survived longer than mice receiving yeast cells alone (p < 0.022), although all mice died. Mice given CRP died faster than mice receiving yeast cells alone (p < 0.017). Miridesap is a molecule that avidly binds SAP, following which the complex is broken down by the liver. Miridesap administered in the drinking water removed SAP from the serum and yeast cells and significantly prolonged the life of mice (p < 0.020). Some were “cured” of candidiasis. SAP administered early in the septic process provided short-lived benefit to mice, probably by blunting cytokine secretion associated with disseminated candidiasis. The most important finding was that removal of SAP with miridesap led to prolonged survival by removing SAP and preventing its dampening effects on the host immune response.
Insight into Potential Biomedical Application of Mesoporous Materials
Authors: Spiridon Irene Alexandra , Căruntu Irina Draga , Spiridon Iuliana , Brăescu Radu
Published: November 2022

The physicochemical properties of many drugs have a decisive impact on their bioavailability, as well as the pharmacokinetic efficiency in various disease therapeutics. That is why mesoporous materials have attracted a special interest in the drug delivery field, facilitating the loading of drugs into their pores due to their high surface area and porosity. The interfacial interactions established with drug molecules represent the driving force for efficient drug loading and controlled release kinetics. Moreover, these materials offer an optimal design for implantable local-delivery devices or for improving the accuracy of imaging techniques in clinical diagnosis. Their use is validated by improvements in therapeutic outcome and prevention of side effects. This review discusses the role of mesoporous materials in different biomedical applications.
Target Prediction of 5,10,15,20-Tetrakis(4′-Sulfonatophenyl)-Porphyrin Using Molecular Docking
Authors: Udrea Ana-Maria , Dinache Andra , Staicu Angela , Avram Speranta
Published: November 2022

Photodynamic therapy has the potential to be a new and effective cancer treatment. Even if in vitro and in vivo research show promise, the molecular mechanism remains unclear. In this study, molecular docking simulations predict the binding affinity of the 5,10,15,20-tetrakis(4′-sulfonatophenyl)-porphyrin tetraammonium photosensitizer on several potential targets in photodynamic treatment. Our results indicate that this photosensitizer binds to several receptor targets, including B-cell lymphoma 2 (BCL-2) and other related proteins BCL-xL, MCL-1, or A1. The binding affinity of the porphyrin derivative with human serum albumin was determined using UV–vis absorption spectroscopy and predicted using molecular docking. We conclude that the studied porphyrin photosensitizer binds to human serum albumin and may inhibit the cancer cell line through its interactions with HIS and MET AA residues from BCL-2, MCL-1, and β-catenin receptors or through its low estimated free energy of binding when interacting with A1 and BCL-B receptors.
Spatially Separated Heterodyne Grating Interferometer for In-Plane and Out-of-Plane Displacement Measurements
Authors: Chang Di , Yin Ziqi , Sun Yunke , Hu Pengcheng , Tan Jiubin , Fan Zhigang
Published: November 2022

Grating interferometers that measure in-plane and out-of-plane displacements are not only effective two-degree-of-freedom (DOF) sensors, but are also basic units of six-DOF measurement systems. Besides resolution and accuracy, periodic nonlinear errors, misalignment tolerance, and size of reading heads are more crucial than ever. In this work, a spatially separated heterodyne grating interferometer that measures in- and out-of-plane displacements is proposed. A prototype with 3 mm diameter beams with a size of 69 mm × 51 mm × 41 mm was built and tested. The experiment results show that the 30 s stability is 2.5 nm; the periodic nonlinear errors of the two measuring directions are less than the resolutions (0.25 nm for in-plane motions and 0.15 nm for out-of-plane motions). Double-diffracted configuration ensures that the misalignment tolerances are three axes larger than ±2 mrad.
Broadband Signal Digitization Based on Low-Speed Non-Uniform Photonic Sampling
Authors: Lyu Weiqiang , Li Zhengkai , Zhang Lingjie , Tian Huan , Fu Zhenwei , Zhang Zhiyao , Sun Bao , Zhang Yali , Zhang Shangjian , Li Heping , Liu Yong
Published: November 2022

A new non-uniform photonic sampling (NPS) strategy and its special signal reconstruction algorithm are proposed to achieve digital acquisition of broadband periodic signals at a low sampling rate. Compared with the existing schemes, the NPS strategy can largely reduce the sampling number to acquire identical signal information as that obtained by using its equivalent high-speed uniform photonic sampling, which is beneficial for reducing the sampling time and the data volume of the NPS-based analog-to-digital converter (ADC). In addition, the calculation time of the proposed algorithm is millions of times lower than that of the digital alias-free signal processing (DASP) algorithm used before, which benefits from the fast Fourier transform calculation of a one-dimensional data array instead of a two-dimensional data array calculation in the DASP algorithm. A simulation is performed to validate the feasibility of the proposed scheme. In the simulation, a single-channel NPS-based ADC with an average sampling rate of 1 GSa/s is demonstrated by using the proposed NPS strategy and signal reconstruction algorithm. The results indicate the reconstructed signal information for a single-tone microwave signal at 9.9 GHz and a linear frequency modulation signal in the frequency range of 1 GHz to 9 GHz are identical to those obtained by using its equivalent high-speed uniform photonic sampling-based ADC with a sampling rate of 20 GSa/s.
Effect of Dual-Pulse Temporal Shaping on Ultraviolet Nanosecond Laser Damage of Fused Silica Surface in High Fluence Regime
Authors: Liu Wenfeng , Sun Mingying , Guo Yajing , Shi Yiqun , Xu Yingming , Jiao Zhaoyang , Cui Zijian , Zhu Jianqiang
Published: November 2022

We present the effect of dual-pulse temporal shaping on the ultraviolet nanosecond laser damage characteristics of a fused silica exit surface in a high fluence regime. The pre- and post-pulse have the opposite effects on the damage behavior at a pulse delay of 20 ns. The pre-pulse irradiation significantly increases the main-pulse threshold, making it much higher than that of the single-pulse threshold, while the post-pulse has little effect on the main-pulse threshold. For near-threshold damage sites, the pre-pulse reduces the average damage size and depth, making them smaller than those of the single-pulse, while the post-pulse drastically increases the average size and depth, making them much larger than those of the single-pulse. The average size of the damage site is monotonously increased from 43.6 µm to 127.9 µm with increasing post-pulse energy. For the pre-pulse with a shape factor of 0.61, the damage threshold of the main pulse increases with increasing delay and nearly stabilizes after 10 ns. The underlying mechanism of the temporal-shaping effect on laser damage is discussed based on the applied precursor modification to absorption enhancement, which could provide insights for studying ultraviolet laser damage of fused silica optics.
Endophytes from Halotolerant Plants Aimed to Overcome Salinity and Draught
Authors: Chebotar Vladimir K. , Chizhevskaya Elena P. , Baganova Maria E. , Keleinikova Oksana V. , Yuzikhin Oleg S. , Zaplatkin Alexander N. , Khonina Olesya V. , Kostitsin Roman D. , Lapenko Nina G.
Published: November 2022

The aim of our research was to study the endosphere of four halophytic plants: Salicornia europaea L., Salsola australis (R.Br.), Bassia sedoides (Pall.) and Kochia prostrata (L.) Schrad. from arid and saline areas of the Stavropol Territory, Russia. In total, 28 endophyte strains were isolated from the roots and stems of these halophytic plants. Most of the isolates (23 out of 28) were identified as Bacillus sp. while others belonged to the genera Oceanobacillus, Paenibacillus, Pantoea, Alcaligenes and Myroides. Three strains of Bacillus sp. (Se5R, Se1-1R, and Se1-3S), isolated from the S. europaea were capable of growth at 55 °C and in 10% of NaCl. Strains Se1-4S, Kp20-2S, and Bs11-2S Bacillus sp. (isolated from the S. australis, K. prostrata and B. sedoides, respectively) demonstrated strong plant growth promoting activity: 85–265% over control lettuce plants and a high degree of growth suppression (59.1–81.2%) of pathogenic fungi Fusarium oxysporum, Bipolaris sorokiniana and Rhizoctonia solani. Selected strains can be promising candidates for the development of bioinoculants to facilitate salt soil phytoremediation and be beneficial for mitigating the salt stress to the plants growing in salt-affected habitats.
Exogenous Proline Improves Salt Tolerance of Alfalfa through Modulation of Antioxidant Capacity, Ion Homeostasis, and Proline Metabolism
Authors: Guo Shuaiqi , Ma Xuxia , Cai Wenqi , Wang Yuan , Gao Xueqin , Fu Bingzhe , Li Shuxia
Published: November 2022

Alfalfa (Medicago sativa L.) is an important forage crop, and its productivity is severely affected by salt stress. Although proline is a compatible osmolyte that plays an important role in regulating plant abiotic stress resistance, the basic mechanism of proline requires further clarification regarding the effect of proline in mitigating the harmful effects of salinity. Here, we investigate the protective effects and regulatory mechanisms of proline on salt tolerance of alfalfa. The results show that exogenous proline obviously promotes seed germination and seedling growth of salt-stressed alfalfa. Salt stress results in stunted plant growth, while proline application alleviates this phenomenon by increasing photosynthetic capacity and antioxidant enzyme activities and decreasing cell membrane damage and reactive oxygen species (ROS) accumulation. Plants with proline treatment maintain a better K+/Na+ ratio by reducing Na+ accumulation and increasing K+ content under salt stress. Additionally, proline induces the expression of genes related to antioxidant biosynthesis (Cu/Zn-SOD and APX) and ion homeostasis (SOS1, HKT1, and NHX1) under salt stress conditions. Proline metabolism is mainly regulated by ornithine-δ-aminotransferase (OAT) and proline dehydrogenase (ProDH) activities and their transcription levels, with the proline-treated plants displaying an increase in proline content under salt stress. In addition, OAT activity in the ornithine (Orn) pathway rather than Δ1-pyrroline-5-carboxylate synthetase (P5CS) activity in the glutamate (Glu) pathway is strongly increased under salt stress, made evident by the sharp increase in the expression level of the OAT gene compared to P5CS1 and P5CS2. Our study provides new insight into how exogenous proline improves salt tolerance in plants and that it might be used as a significant practical strategy for cultivating salt-tolerant alfalfa.
Rheological Behaviors and Damage Mechanism of Asphalt Binder under the Erosion of Dynamic Pore Water Pressure Environment
Authors: Wang Wentao , Zhao Kang , Xie Tingting , Liu Huifang , Zhao Guanyi , Wang Linbing
Published: November 2022

Asphalt binder plays an important role in the overall resistance of asphalt mixture to the moisture damage induced by a dynamic pore water pressure environment. This study evaluates the moisture sensitivity of asphalt binder from the perspective of rheological behaviors using the dynamic shear rheometer (DSR) and the bending beam rheometer (BBR) methods at high, medium, and low temperatures. The damage mechanism is further discussed quantitatively based on the Fourier transform infrared spectroscopy (FTIR) method. The results indicate that a longer conditioning duration is beneficial for asphalt binder to recover its adhesion at 60 °C in multiple stress creep recover (MSCR) tests, but the increasing pore water pressure magnitude of 60 psi held an opposite effect in this study. The asphalt binder’s fatigue life at 20 °C in linear amplitude sweep (LAS) tests decreased obviously with conditioning duration and environmental severity, but the reducing rate gradually slowed down, while the groups of 50 psi—4000 cycles and 60 psi—4000 cycles held a comparable erosion effect. Both the stiffness and relaxation moduli at −12 °C in the BBR tests exhibited an obvious decreasing trend with conditioning duration and environmental severity. The erosion effect on the asphalt binder was gradually enhanced, but it also exhibited a slightly more viscous performance. Water conditioning induced several obvious characteristic peaks in the FTIR absorbance spectra of the asphalt binder. The functional group indexes presented a trend of non-monotonic change with conditioning duration and environmental severity, which made the asphalt binder show complicated rheological behaviors, such as non-monotonic variations in performance and the abnormal improving effect induced by dynamic pore water pressure conditioning.
Graphene/Polymer Nanocomposites: Preparation, Mechanical Properties, and Application
Authors: Lee Se Jung , Yoon Seo Jeong , Jeon In-Yup
Published: November 2022

Although polymers are very important and vastly used materials, their physical properties are limited. Therefore, they are reinforced with fillers to relieve diverse restrictions and expand their application areas. The exceptional properties of graphene make it an interesting material with huge potential for application in various industries and devices. The interfacial interaction between graphene and the polymer matrix improved the uniform graphene dispersion in the polymer matrix, enhancing the general nanocomposite performance. Therefore, graphene functionalization is essential to enhance the interfacial interaction, maintain excellent properties, and obstruct graphene agglomeration. Many studies have reported that graphene/polymer nanocomposites have exceptional properties that enable diverse applications. The use of graphene/polymer nanocomposites is expected to increase sustainably and to transform from a basic to an advanced material to offer optimum solutions to industry and consumers.
Radiation Processing of Styrene-isoprene-styrene/Poly(ε-caprolactone) Blends
Authors: Lungulescu Eduard-Marius , Zaharescu Traian
Published: November 2022

The irradiation consequences on styrene-isoprene-styrene (SIS)/poly(ε-caprolactone) (PCL) blends are discussed starting from the oxidation initiation. Three characterization methods: chemiluminescence, differential scanning calorimetry and FTIR spectroscopy are applied. The differences that exist between the two components are revealed, when the oxidation rates of the inspected formulas depend on the blending proportion and the degradation conditions. The relevant activation energies characterizing the oxidation strength as well as the kinetic parameters of degradation during the accelerated damaging of blended polymers are related to the inhibition protection of PCL on the faster oxidation of SIS. The interaction between mixed components is revealed by the structural modifications simultaneously accompanied by the competition of formation and decay of radicals.
Fatigue Crack Propagation Prediction of Corroded Steel Plate Strengthened with Carbon Fiber Reinforced Polymer (CFRP) Plates
Authors: Li Anbang , Wang Lu , Xu Shanhua
Published: November 2022

The purpose of this study is to investigate the mechanism of improving fatigue performance and the estimation model of fatigue life for corroded steel plate strengthened with CFRP plates. A new two-stage fatigue crack propagation prediction model for the corroded steel plate strengthened with CFRP plates was proposed; moreover, the identification of critical rust pits and the equivalent method of initial cracks, and the calculation method of stress intensity factor (SIF) values at the crack tip were established. The accuracy of the proposed model was verified by comparing the predicted and tested fatigue life of the corroded steel plate strengthened with CFRP plates. Finally, the proposed two-stage crack propagation model was applied to carry out a parameter analysis to investigate the effect of weight loss rate, equivalent initial crack size, adhesive thickness, CFRP stiffness and CFRP prestress level on the fatigue crack propagation of the corroded steel plate strengthened with CFRP plates. Results showed that the maximum depth and the average width of the rust pits were suggested to be taken as the equivalent dimensions of the initial semi-elliptical surface crack for the fatigue crack propagation prediction of corroded steel plate strengthened with CFRP plates. Increasing the weight loss rate of the corroded steel plate, the initial crack size or the adhesive thickness would accelerate the crack growth and reduce the fatigue life, whereas increasing the stiffness or prestress level of the CFRP plate would significantly reduce the crack growth rate and increase the fatigue life. The smaller the initial crack size, the more sensitive the crack propagation life was to the variation of equivalent initial crack size. The influence of adhesive thickness on the fatigue life was limited and convergent, and the application of prestressing could significantly improve the utilization rate of CFRP materials and the fatigue strengthening effect of the corroded steel plate.
Evaluating the Injection Moulding of Plastic Parts Using In Situ Time-Resolved Small-Angle X-ray Scattering Techniques
Authors: Costa André A. , Gameiro Fábio , Potêncio Artur , Silva Daniel P. da , Carreira Pedro , Martinez Juan Carlos , Pascoal-Faria Paula , Mateus Artur , Mitchell Geoffrey R.
Published: November 2022

In this study, we describe the design and fabrication of an industrial injection moulding system that can be mounted and used on the NCD-SWEET small-angle X-ray scattering beamline at ALBA. We show how highly useful time-resolved data can be obtained using this system. We are able to evaluate the fraction of the material in the mould cavity and identify the first material to solidify and how this varies with the injection temperature. The design follows current industrial practice and provides opportunities to collect time-resolved data at several points within the mould cavity so that we can build up a 4D perspective of the morphology and its temporal development. The quantitative data obtained will prove invaluable for the optimisation of the next generation of injection moulding techniques. This preliminary work used results from the injection moulding of a general-purpose isotactic polypropylene.