bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Compost Extract Addition to Different Types of Fertilizers on Quality at Harvest and Shelf Life of Spinach
Authors: Giménez Almudena , Gómez Perla A. , Bustamante María Ángeles , Pérez-Murcia María Dolores , Martínez-Sabater Encarnación , Ros Margarita , Pascual José A. , Egea-Gilabert Catalina , Fernández Juan A.
Published: March 2021

Spinach is rich in minerals, vitamins, phytochemicals and bioactive compounds with health-beneficial effects; however, this plant also tends to accumulate oxalates and nitrates in their leaves. Apart from genotype, nutrition is the pre-harvest factor that mostly affects quality attributes at harvest. Particularly, the application of compost extracts (CE) may induce resistance against soil-borne diseases and favour secondary metabolism, increasing antioxidant capacity. The objective of this study was to evaluate the effects of different types of fertilization with or without the addition of CE, on harvest quality and shelf life of minimally processed spinach (Spinacia oleracea, var. Shrike RZ) stored during 12 days at 4 °C. A compost extract (CE) was prepared by mixing a compost from agri-food wastes (vine pruning, leek waste and olive mill waste) with deionized water. CE foliar applications were done from days 28 and 56 after sowing. The treatments applied were: Control; Control + CE; NPK (inorganic NPK fertilizer 15-15-15); NPK + CE; DMPP (ENTEC Nitrofoska® plus the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP)) and DMPP + CE. After harvest, spinach leaves were minimally processed and packaged to generate a passive modified atmosphere. Nitrate content in the control treatment was reduced by the addition of CE, although in the rest of the treatments, CE addition did not produce any effect. For nitrite contents, the lowest value was obtained for the Control + CE. Moreover, the oxalate content was the lowest for the control treatment with a decreasing trend throughout the storage. The treatment Control + CE also showed the highest initial total phenolic contents, with very similar values at the end of shelf life to those observed at harvest for all the treatments. The highest differences in color as regards the initial values were detected for DMPP. Microbial loads increased for all the treatments without differences between them. The atmosphere reached at the end of the cold storage was the same for all the cases, with CO2 and O2 around 10 kPa for each one of them. After 12 days at 4 °C, all the treatments were above the limit of usability, with the spinach leaves acceptable for consumption. The results found in this study indicate that the addition of CE might be convenient for obtaining spinach rich in bioactive compounds and with low concentrations of antinutritional factors, without affecting the microbial load of the final product.
Comparative Performance Evaluation of Commercial Packing Materials for Malodorants Abatement in Biofiltration
Authors: Lebrero Raquel , Rodríguez Elisa , Collantes María , De Juan Carlos , Norden Geir , Rosenbom Kim , Muñoz Raúl
Published: March 2021

Packing materials used in biofiltration of gaseous pollutants represent a key design parameter, as a proper selection might not only determine the adequate performance of the system but also its cost-effectiveness. This study systematically assessed and compared the performance of a conventional plastic carrier with that of two novel clay-based materials from SAINT GOBAIN for the abatement of a model odorous stream composed of H2S, methylmercaptan and toluene. The packing materials were tested in biotrickling filters, biofilters and a two-phase biotrickling filter. SAINT GOBAIN materials exhibited a higher adsorption potential under abiotic conditions, a higher buffer capacity and a superior performance compared to conventional plastic rings when implemented in biotrickling filters operating at gas residence times as low as 7.5 s. Among the materials tested in biofilters, Filtralite Air AC supported almost complete H2S and toluene removals at a gas residence time of 20 s, while successfully eliminating methylmercaptan at values of ~80%. Interestingly, under most of the conditions tested, clay-based materials also showed comparable pressure drop values than those of plastic rings.
An Improved Hilbert–Huang Transform for Vibration-Based Damage Detection of Utility Timber Poles
Authors: Das Ipshita , Arif Mohammad Taufiqul , Oo Aman Maung Than , Subhani Mahbube
Published: March 2021

In this study, vibration based non-destructive testing (NDT) technique is adopted for assessing the condition of in-service timber pole. Timber is a natural material, and hence the captured broadband signal (induced from impact using modal hammer) is greatly affected by the uncertainty on wood properties, structure, and environment. Therefore, advanced signal processing technique is essential in order to extract features associated with the health condition of timber poles. In this study, Hilbert–Huang Transform (HHT) and Wavelet Packet Transform (WPT) are implemented to conduct time-frequency analysis on the acquired signal related to three in-service poles and three unserviceable poles. Firstly, mother wavelet is selected for WPT using maximum energy to Shannon entropy ratio. Then, the raw signal is divided into different frequency bands using WPT, followed by reconstructing the signal using wavelet coefficients in the dominant frequency bands. The reconstructed signal is then further decomposed into mono-component signals by Empirical Mode Decomposition (EMD), known as Intrinsic Mode Function (IMF). Dominant IMFs are selected using correlation coefficient method and instantaneous frequencies of those dominant IMFs are generated using HHT. Finally, the anomalies in the instantaneous frequency plots are efficiently utilised to determine vital features related to pole condition. The results of the study showed that HHT with WPT as pre-processor has a great potential for the condition assessment of utility timber poles.
The Effect of Different Levels of Sodium Nitrate on the Physicochemical Parameters and Nutritional Value of Traditionally Produced Fermented Loins
Authors: Karwowska Małgorzata , Stadnik Joanna , Wójciak Karolina
Published: March 2021

This study evaluated the effect of sodium nitrate reduction on the following parameters: thiobarbituric acid reactive substances, the color parameters (CIE L* a* b*), total heme pigments, heme iron content and nutritional value related to N-nitrosamines content as well as nitrate and nitrite residues of traditionally produced fermented loins. Raw loins (m. longissimus thoracis et lumborum) and fermented products with different levels of nitrate added (0, 50, 100, 150 mg kg−1) were tested during six months of vacuum storage. The reduction of nitrate did not lead to statistically significant changes in total pigment content as well as heme iron content in fermented loins at the end of processing and during storage. Water activity did not differ statistically significantly between the formulations. Fermented loins at the end of processing revealed residual sodium nitrite levels of <10 mg kg−1, while the amount of nitrate residue depended on the level added during production, obtaining the highest value of 19.0 mg kg−1 for the sample with the highest nitrate addition. The level of nitrosamines was <5 µg kg−1 in all samples, which proves their chemical safety. In conclusion, the use of nitrate reduced to 50 mg kg−1 in fermented loins allows to obtain a product with properties similar to the product with 150 mg kg−1 of nitrate, especially in terms of its physicochemical properties and lipid oxidation.
Intra-System Reliability Assessment of 2-Dimensional Shear Wave Elastography
Authors: Edwards Christopher , Cavanagh Erika , Kumar Sailesh , Clifton Vicki , Fontanarosa Davide
Published: March 2021

The availability of 2-Dimensional Shear Wave Elastography (2D-SWE) technology on modern medical ultrasound systems is becoming increasingly common. The technology is now being used to investigate a range of soft tissues and related pathological conditions. This work investigated the reliability of a single commercial 2D-SWE system using a tissue-mimicking elastography phantom to understand the major causes of intra-system variability. Sources of shear wave velocity (SWV) measurement variability relates to imaging depth, target stiffness, sampling technique and the operator. Higher SWV measurement variability was evident with increasing depth and stiffness of the phantom targets. The influence of the operator was minimal, and variations in sampling technique had little impact on the SWV.
Gene Delivery Technologies with Applications in Microalgal Genetic Engineering
Authors: Gutiérrez Sergio , Lauersen Kyle J.
Published: March 2021

Microalgae and cyanobacteria are photosynthetic microbes that can be grown with the simple inputs of water, carbon dioxide, (sun)light, and trace elements. Their engineering holds the promise of tailored bio-molecule production using sustainable, environmentally friendly waste carbon inputs. Although algal engineering examples are beginning to show maturity, severe limitations remain in the transformation of multigene expression cassettes into model species and DNA delivery into non-model hosts. This review highlights common and emerging DNA delivery methods used for other organisms that may find future applications in algal engineering.
PER2 Circadian Oscillation Sensitizes Esophageal Cancer Cells to Chemotherapy
Authors: Redondo Juan Alfonso , Bibes Romain , Vercauteren Drubbel Alizée , Dassy Benjamin , Bisteau Xavier , Maury Eleonore , Beck Benjamin
Published: March 2021

Esophageal squamous cell carcinoma (eSCC) accounts for more than 85% cases of esophageal cancer worldwide and the 5-year survival rate associated with metastatic eSCC is poor. This low survival rate is the consequence of a complex mechanism of resistance to therapy and tumor relapse. To effectively reduce the mortality rate of this disease, we need to better understand the molecular mechanisms underlying the development of resistance to therapy and translate that knowledge into novel approaches for cancer treatment. The circadian clock orchestrates several physiological processes through the establishment and synchronization of circadian rhythms. Since cancer cells need to fuel rapid proliferation and increased metabolic demands, the escape from circadian rhythm is relevant in tumorigenesis. Although clock related genes may be globally repressed in human eSCC samples, PER2 expression still oscillates in some human eSCC cell lines. However, the consequences of this circadian rhythm are still unclear. In the present study, we confirm that PER2 oscillations still occur in human cancer cells in vitro in spite of a deregulated circadian clock gene expression. Profiling of eSCC cells by RNAseq reveals that when PER2 expression is low, several transcripts related to apoptosis are upregulated. Consistently, treating eSCC cells with cisplatin when PER2 expression is low enhances DNA damage and leads to a higher apoptosis rate. Interestingly, this process is conserved in a mouse model of chemically-induced eSCC ex vivo. These results therefore suggest that response to therapy might be enhanced in esophageal cancers using chronotherapy.
Maintaining Inducibility of Dermal Follicle Cells on Silk Fibroin/Sodium Alginate Scaffold for Enhanced Hair Follicle Regeneration
Authors: Dong Kuo , Wang Xinyu , Shen Ying , Wang Yiyu , Li Binbin , Cai Cuiling , Shen Linyi , Guo Yajin
Published: March 2021

The extracellular matrix (ECM) is important for maintaining cell phenotype and promoting cell proliferation and differentiation. In order to better solve the problem of skin appendage regeneration, a combination of mechanical/enzymatic digestion methods was used to self-extract dermal papilla cells (DPCs), which were seeded on silk fibroin/sodium alginate scaffolds as seed cells to evaluate the possibility of skin regeneration/regeneration of accessory organs. Scanning electron microscopy (SEM) graphs showed that the interconnected pores inside the scaffold had a pore diameter in the range of 153–311 μm and a porosity of 41–82%. Immunofluorescence (IF) staining and cell morphological staining proved that the extracted cells were DPCs. The results of a Cell Counting Kit-8 (CCK-8) and Calcein-AM/PI live-dead cell staining showed that the DPCs grew well in the composite scaffold extract. Normal cell morphology and characteristics of aggregation growth were maintained during the 3-day culture, which showed that the silk fibroin/sodium alginate (SF/SA) composite scaffold had good cell-compatibility. Hematoxylin-eosin (H&E) staining of tissue sections further proved that the cells adhered closely and aggregated to the pore wall of the scaffold, and retained the ability to induce differentiation of hair follicles. All these results indicate that, compared with a pure scaffold, the composite scaffold promotes the adhesion and growth of DPCs. We transplanted the SF/SA scaffolds into the back wounds of SD rats, and evaluated the damage model constructed in vivo. The results showed that the scaffold inoculated with DPCs could accelerate the repair of the skin and promote the regeneration of the hair follicle structure.
Structure and Location of Protein Sites Binding Self-Associated Congo Red Molecules with Intercalated Drugs as Compact Ligands—Theoretical Studies
Authors: Magdalena Ptak-Kaczor , Klaudia Kwiecińska , Jacek Korchowiec , Katarzyna Chłopaś , Mateusz Banach , Irena Roterman , Anna Jagusiak
Published: March 2021

In the search for new carriers capable of transporting toxic drugs to a target, particular attention has been devoted to supramolecular systems with a ribbon-like micellar structure of which Congo red is an example. A special promise of the possible use of such systems for directing drugs to a target emerges from their particular affinity to immune complexes and as an independent property, binding many organic compounds including drugs by intercalation. Serum albumin also appeared able to bind micellar particles of such systems. It may protect them against dilution in transport. The mathematical tool, which relies on analysis of the distribution of polarity and hydrophobicity in protein molecules (fuzzy oil drop model), has been used to find the location of binding area in albumin as well as anchorage site for Congo red in heated IgG light chain used as a model presenting immunoglobulin-like structures. Results confirm the suggested formerly binding site of Congo red in V domain of IgG light chain and indicated the cleft between pseudo-symmetric domains of albumin as the area of attachment for the dye.
The Roles of Protein Structure, Taxon Sampling, and Model Complexity in Phylogenomics: A Case Study Focused on Early Animal Divergences
Authors: Pandey Akanksha , Braun Edward L.
Published: March 2021

Despite the long history of using protein sequences to infer the tree of life, the potential for different parts of protein structures to retain historical signal remains unclear. We propose that it might be possible to improve analyses of phylogenomic datasets by incorporating information about protein structure. We test this idea using the position of the root of Metazoa (animals) as a model system. We examined the distribution of “strongly decisive” sites (alignment positions that support a specific tree topology) in a dataset comprising >1500 proteins and almost 100 taxa. The proportion of each class of strongly decisive sites in different structural environments was very sensitive to the model used to analyze the data when a limited number of taxa were used but they were stable when taxa were added. As long as enough taxa were analyzed, sites in all structural environments supported the same topology regardless of whether standard tree searches or decisive sites were used to select the optimal tree. However, the use of decisive sites revealed a difference between the support for minority topologies for sites in different structural environments: buried sites and sites in sheet and coil environments exhibited equal support for the minority topologies, whereas solvent-exposed and helix sites had unequal numbers of sites, supporting the minority topologies. This suggests that the relatively slowly evolving buried, sheet, and coil sites are giving an accurate picture of the true species tree and the amount of conflict among gene trees. Taken as a whole, this study indicates that phylogenetic analyses using sites in different structural environments can yield different topologies for the deepest branches in the animal tree of life and that analyzing larger numbers of taxa eliminates this conflict. More broadly, our results highlight the desirability of incorporating information about protein structure into phylogenomic analyses.
The Building as a Home: Housing Cooperatives in Barcelona
Authors: Avilla-Royo Raül , Jacoby Sam , Bilbao Ibon
Published: March 2021

The recent growth of cooperative housing in Spain questions existing design standards and regulations as well as cultural norms of ownership, management and current housing typologies. This paper analyzes the design opportunities and challenges emerging from this. It studies the transformative capacity of housing cooperatives and how the realization of new social, spatial and economic demands is restricted by regulatory and administrative frameworks that limit collective ownership and use. Based on a case study analysis of recent projects in Barcelona, the paper discusses how regulations condition housing design, but also why changing ideas of ownership, household and dwelling structures require a review of how regulations are formulated and implemented. It examines this in the context of designing with housing cooperatives and their ethos defined by engagement in and responsibility for all decision-making processes and self-management. In cooperative housing, architecture is a process, not a product, one that extends beyond the completion of a building. This gives credibility to the claim of cooperative housing not just as a grassroots response to housing failures, but also as a political project of democratization and social transformation.
Amyloid Precursor-like Protein 2 Expression Increases during Pancreatic Cancer Development and Shortens the Survival of a Spontaneous Mouse Model of Pancreatic Cancer
Authors: Poelaert Brittany J. , Knoche Shelby M. , Larson Alaina C. , Pandey Poomy , Seshacharyulu Parthasarathy , Khan Nuzhat , Maurer H. Carlo , Olive Kenneth P. , Sheinin Yuri , Ahmad Rizwan , Singh Amar B. , Batra Surinder K. , Rachagani Satyanarayana , Solheim Joyce C.
Published: March 2021

In the United States, pancreatic cancer is a major cause of cancer-related deaths. Although substantial efforts have been made to understand pancreatic cancer biology and improve therapeutic efficacy, patients still face a bleak chance of survival. A greater understanding of pancreatic cancer development and the identification of novel treatment targets are desperately needed. Our analysis of gene expression data from patient samples showed an increase in amyloid precursor-like protein 2 (APLP2) expression within primary tumor epithelium relative to pancreatic intraepithelial neoplasia (PanIN) epithelial cells. Augmented expression of APLP2 in primary tumors compared to adjacent stroma was also observed. Genetically engineered mouse models of spontaneous pancreatic ductal adenocarcinoma were used to investigate APLP2′s role in cancer development. We found that APLP2 expression intensifies significantly during pancreatic cancer initiation and progression in the LSL-KrasG12D/+; LSL-Trp53R172H/+; Pdx-1-Cre (KPC) mouse model, as shown by immunohistochemistry analysis. In studies utilizing pancreas-specific heterozygous and homozygous knockout of APLP2 in the KPC mouse model background, we observed significantly prolonged survival and reduced metastatic progression of pancreatic cancer. These results demonstrate the importance of APLP2 in pancreatic cancer initiation and metastasis and indicate that APLP2 should be considered a potential therapeutic target for this disease.
Mechanisms of Immune Evasion in Acute Lymphoblastic Leukemia
Authors: Pastorczak Agata , Domka Krzysztof , Fidyt Klaudyna , Poprzeczko Martyna , Firczuk Malgorzata
Published: March 2021

Acute lymphoblastic leukemia (ALL) results from a clonal expansion of abnormal lymphoid progenitors of B cell (BCP-ALL) or T cell (T-ALL) origin that invade bone marrow, peripheral blood, and extramedullary sites. Leukemic cells, apart from their oncogene-driven ability to proliferate and avoid differentiation, also change the phenotype and function of innate and adaptive immune cells, leading to escape from the immune surveillance. In this review, we provide an overview of the genetic heterogeneity and treatment of BCP- and T-ALL. We outline the interactions of leukemic cells in the bone marrow microenvironment, mainly with mesenchymal stem cells and immune cells. We describe the mechanisms by which ALL cells escape from immune recognition and elimination by the immune system. We focus on the alterations in ALL cells, such as overexpression of ligands for various inhibitory receptors, including anti-phagocytic receptors on macrophages, NK cell inhibitory receptors, as well as T cell immune checkpoints. In addition, we describe how developing leukemia shapes the bone marrow microenvironment and alters the function of immune cells. Finally, we emphasize that an immunosuppressive microenvironment can reduce the efficacy of chemo- and immunotherapy and provide examples of preclinical studies showing strategies for improving ALL treatment by targeting these immunosuppressive interactions.
Remembering Prof. Dr. Peter Grunwald (1941–2020)
Authors: Topakas Evangelos
Published: March 2021

On 28 December 2020, Prof. Dr. Peter Grunwald died after a short, serious illness. [...]
Total Oxidation of Methane on Oxide and Mixed Oxide Ceria-Containing Catalysts
Authors: Stoian Marius , Rogé Vincent , Lazar Liliana , Maurer Thomas , Védrine Jacques C. , Marcu Ioan-Cezar , Fechete Ioana
Published: March 2021

Methane, discovered in 1766 by Alessandro Volta, is an attractive energy source because of its high heat of combustion per mole of carbon dioxide. However, methane is the most abundant hydrocarbon in the atmosphere and is an important greenhouse gas, with a 21-fold greater relative radiative effectiveness than CO2 on a per-molecule basis. To avoid or limit the formation of pollutants that are dangerous for both human health and the atmospheric environment, the catalytic combustion of methane appears to be one of the most promising alternatives to thermal combustion. Total oxidation of methane, which is environmentally friendly at much lower temperatures, is believed to be an efficient and economically feasible way to eliminate pollutants. This work presents a literature review, a statu quo, on catalytic methane oxidation on transition metal oxide-modified ceria catalysts (MOx/CeO2). Methane was used for this study since it is of great interest as a model compound for understanding the mechanisms of oxidation and catalytic combustion on metal oxides. The objective was to evaluate the conceptual ideas of oxygen vacancy formation through doping to increase the catalytic activity for methane oxidation over CeO2. Oxygen vacancies were created through the formation of solid solutions, and their catalytic activities were compared to the catalytic activity of an undoped CeO2 sample. The reaction conditions, the type of catalysts, the morphology and crystallographic facets exposing the role of oxygen vacancies, the deactivation mechanism, the stability of the catalysts, the reaction mechanism and kinetic characteristics are summarized.
Effect of Multispecies Microbial Consortia on Microbially Influenced Corrosion of Carbon Steel
Authors: Phan Hoang C. , Blackall Linda L. , Wade Scott A.
Published: March 2021

Microbially influenced corrosion (MIC) is responsible for significant damage to major marine infrastructure worldwide. While the microbes responsible for MIC typically exist in the environment in a synergistic combination of different species, the vast majority of laboratory-based MIC experiments are performed with single microbial pure cultures. In this work, marine grade steel was exposed to a single sulfate reducing bacterium (SRB, Desulfovibrio desulfuricans) and various combinations of bacteria (both pure cultures and mixed communities), and the steel corrosion studied. Differences in the microbial biofilm composition and succession, steel weight loss and pitting attack were observed for the various test configurations studied. The sulfate reduction phenotype was successfully shown in half-strength marine broth for both single and mixed communities. The highest corrosion according to steel weight loss and pitting, was recorded in the tests with D. desulfuricans alone when incubated in a nominally aerobic environment. The multispecies microbial consortia yielded lower general corrosion rates compared to D. desulfuricans or for the uninoculated control.
Habitat and Landform Types Drive the Distribution of Carabid Beetles at High Altitudes
Authors: Gobbi Mauro , Armanini Marco , Boscolo Teresa , Chirichella Roberta , Lencioni Valeria , Ornaghi Simone , Mustoni Andrea
Published: March 2021

The high altitude mountain slopes of the Dolomites (Italian Alps) are characterized by great habitat and landform heterogeneities. In this paper, we investigated the effect of Nature 2000 habitat and landform types in driving the high altitude ground beetle (Carabidae) distribution in the Western Dolomites (Brenta group, Italy). We studied the carabid assemblages collected in 55 sampling points distributed in four Nature 2000 habitat types and four landform types located between 1860 and 2890 m above sea level (a.s.l.). Twenty-two species, half of them Alpine endemics, were sampled. Species richness and taxonomic distinctness did not show any significant difference among habitat types; conversely, these differences became significant when the landform type was considered. Total activity density and the frequency of brachypterous, endemic and predatory species showed significant differences between both habitat and landform types. Indicator species analysis identified twelve species linked to a specific habitat type and thirteen species linked to a specific landform type. Canonical correspondence analysis showed that altitude and vegetation cover drove the species distribution in each habitat and landform type while the aspect had a weak effect. Our results highlight the need for a geomorphological characterization of the sampling points when high altitude ground-dwelling arthropods are investigated.
A Data-Mining Approach for Wind Turbine Fault Detection Based on SCADA Data Analysis Using Artificial Neural Networks
Authors: Santolamazza Annalisa , Dadi Daniele , Introna Vito
Published: March 2021

Wind energy has shown significant growth in terms of installed power in the last decade. However, one of the most critical problems for a wind farm is represented by Operation and Maintenance (O&M) costs, which can represent 20–30% of the total costs related to power generation. Various monitoring methodologies targeted to the identification of faults, such as vibration analysis or analysis of oils, are often used. However, they have the main disadvantage of involving additional costs as they usually entail the installation of other sensors to provide real-time control of the system. In this paper, we propose a methodology based on machine learning techniques using data from SCADA systems (Supervisory Control and Data Acquisition). Since these systems are generally already implemented on most wind turbines, they provide a large amount of data without requiring extra sensors. In particular, we developed models using Artificial Neural Networks (ANN) to characterize the behavior of some of the main components of the wind turbine, such as gearbox and generator, and predict operating anomalies. The proposed method is tested on real wind turbines in Italy to verify its effectiveness and applicability, and it was demonstrated to be able to provide significant help for the maintenance of a wind farm.
Refrigeration of COVID-19 Vaccines: Ideal Storage Characteristics, Energy Efficiency and Environmental Impacts of Various Vaccine Options
Authors: Santos Alexandre F. , Gaspar Pedro D. , de Souza Heraldo J. L.
Published: March 2021

This article considers the ideal storage conditions for multiple vaccine brands, such as Pfizer, Moderna, CoronaVac, Oxford–AstraZeneca, Janssen COVID-19 and Sputnik V. Refrigerant fluid options for each storage condition, thermal load to cool each type of vaccine and environmental impacts of refrigerants are compared. An energy simulation using the EUED (energy usage effectiveness design) index was developed. The Oxford–AstraZeneca, Janssen COVID-19 and CoronaVac vaccines show 9.34-times higher energy efficiency than Pfizer. In addition, a TEWI (total equivalent warming impact) simulation was developed that prioritizes direct environmental impacts and indirect in refrigeration. From this analysis, it is concluded that the cold storage of Oxford–AstraZeneca, Janssen COVID-19 and CoronaVac vaccines in Brazil generates 35-times less environmental impact than the Pfizer vaccine.
Power Line Communications for Automotive High Voltage Battery Systems: Channel Modeling and Coexistence Study with Battery Monitoring
Authors: Landinger Thomas F. , Schwarzberger Guenter , Hofer Guenter , Rose Matthias , Jossen Andreas
Published: March 2021

As electric vehicles are gaining increasing worldwide interest, advances in driving range and safety become critical. Modern automotive battery management systems (BMS) compete with challenging performance and safety requirements and need to monitor a large amount of battery parameters. In this paper, we propose power line communications (PLC) for high voltage (HV) traction batteries to reduce the BMS wiring effort. By modeling a small-scale battery pack for frequencies up to 300 MHz, we predict the PLC channel transfer characteristics and validate the results using a PLC hardware demonstrator employing a narrowband single-carrier modulation. The results demonstrate that battery PLC is a demanding task due to low access impedances and cell coupling effects, yet transfer characteristics can be improved by optimal impedance matching. PLC for HV BMS not only saves weight and cost, but also improves flexibility in BMS design. PLC enables single-cell monitoring techniques such as online electrochemical impedance spectroscopy (EIS) without additional wiring. Online EIS can be used for in-situ state and temperature estimation saving extra sensors. This work unveils possible coexistence issues between PLC and battery monitoring. In particular, we demonstrate that certain PLC data or packet rates have to be avoided not to interfere with EIS measurements.
The Impact of Smart Prepaid Metering on Non-Technical Losses in Ghana
Authors: Otchere-Appiah Gideon , Takahashi Shingo , Yeboah Mavis Serwaa , Yoshida Yuichiro
Published: March 2021

The high incidence of electricity theft, meter tampering, meter bypassing, reading errors, and defective and aged meters, among others, increases utility losses, especially non-technical losses (NTL). A utility in Ghana piloted a non-technical loss reduction program in 2019 to replace postpaid meters with anti-tamper, anti-fraud, and anti-theft smart prepaid meters. By using customer-level residential billing panel data from 2018 to 2019 obtained from the utility, we assess the effectiveness of this program using the difference-in-differences fixed-effect approach. On average, the results indicated that the reported amount of customers’ monthly electricity consumption increases by 13.2% when any tampered postpaid meter is replaced with a smart prepaid meter, indicating the NTLs by customers. We further employed quantile difference-in-differences regression and observed that reported energy consumption has increased for all households except those at the lower quantile (25th quantile). We conclude that smart prepaid metering could be a remedy to reduce NTLs for the electricity distribution sector in areas where electricity theft is rampant.
Application of Fractional-Order Calculus to Improve the Mathematical Model of a Two-Mass System with a Long Shaft
Authors: Lozynskyy Andriy , Chaban Andriy , Perzyński Tomasz , Szafraniec Andrzej , Kasha Lidiia
Published: March 2021

Based on the general theory of fractional order derivatives and integrals, application of the Caputo–Fabrizio operator is analyzed to improve a mathematical model of a two-mass system with a long shaft and concentrated parameters. Thus, the real transmission of complex electric drives, which consist of long shafts with a sufficient degree of adequacy, is presented as a two-mass system. Such a system is described by ordinary fractional order differential equations. In addition, it is well known that an elastic mechanical wave, propagating along a drive transmission with a long stiff shaft, creates a retardation effect on distribution of the time–space angular velocity, the rotation angle of the shaft, and its elastic moment. The approach proposed in the current work helps to take in account the moving elastic wave along the shaft of electric drive mechanism. On this basis, it is demonstrated that the use of the fractional order integrator in the model for the elastic moment enables it to reproduce real transient processes in the joint coordinates of the system. It also provides an accuracy equivalent to the model with distributed parameters. The distance between the traditional model and the model in which the fractional integral is used for the elastic moment modelling in a two-mass system, with a long shaft, is analyzed.
Comparison of Topsoil Organic Carbon Stocks on Slopes under Soil-Protecting Forests in Relation to the Adjacent Agricultural Slopes
Authors: Wiśniewski Paweł , Märker Michael
Published: March 2021

Soil erosion is one of the major processes degrading the natural environment but also agricultural production areas. Soil erosion may lead to soil organic carbon (SOC) loss, especially from sloping agricultural terrain units. The use of phytomelioration in environmental management, particularly long-term, permanent forest vegetation, is widely recognized as a possible measure for soil erosion protection and mitigation of climate change through carbon sequestration. The aim of this study was to compare of the topsoil organic carbon stocks on the slopes under soil-protecting forests in relation to the adjacent agricultural slopes. The research was conducted in the young glacial landscape of North-Central Poland. The study indicated the significant role of forest management on the increase of soil organic matter content and SOC stock. The results show that land use and slope gradients are important factors controlling soil organic carbon pools in topsoil in young glacial areas. This topic is extremely important particularly as the effects of climate change become more and more visible, and society faces new challenges in preventing these changes.
Spatial Variability of Throughfall in a Larch (Larix gmelinii) Forest in Great Kingan Mountain, Northeastern China
Authors: Sheng Houcai , Cai Tijiu
Published: March 2021

Larix gmelinii forest is one of the dominant forest types in boreal forest and plays a unique eco-hydrological role in the terrestrial ecosystem. However, the throughfall variability in boreal forest ecosystems, which plays a crucial role in regulating hydrology, remains unclear. Here, we investigated the spatial variability and temporal stability of throughfall within a Larix gmelinii forest in the full leaf stage in Great Kingan Mountain, Northeast China, and the effects of rainfall properties and canopy structure on throughfall variability were systematically evaluated. The results indicate that throughfall represented 81.26% of the gross rainfall in the forest. The throughfall CV (coefficient of variation of throughfall) had a significant and negative correlation with the rainfall amount, rainfall intensity, rainfall duration, and distance from the nearest trunk, whereas it increased with increasing canopy thickness and LAI (leaf area index). The correlation analysis suggested that the throughfall variability was mainly affected by the rainfall amount (R2 = 0.7714) and canopy thickness (R2 = 0.7087). The temporal stability analysis indicated that the spatial distribution of the throughfall was temporally stable. Our findings will facilitate a better understanding of the spatiotemporal heterogeneity of throughfall and help the accurate assessment of throughfall and soil water within boreal forests.
Measuring a Fire. The Story of the January 2019 Fire Told from Measurements at the Warra Supersite, Tasmania
Authors: Wardlaw Tim
Published: March 2021

Non-stand-replacing wildfires are the most common natural disturbance in the tall eucalypt forests of Tasmania, yet little is known about the conditions under which these fires burn and the effects they have on the forest. A dry lightning storm in January 2019 initiated the Riveaux Road fire. This fire burnt nearly 64,000 ha of land, including tall eucalypt forests at the Warra Supersite. At the Supersite, the passage of the fire was recorded by a suite of instruments measuring weather conditions and fluxes (carbon, water and energy), while a network of permanent plots measured vegetation change. Weather conditions in the lead-up and during the passage of the fire through the Supersite were mild—a moderate forest fire danger index. The passage of the fire through the Supersite caused a short peak in air temperature coinciding with a sharp rise in CO2 emissions. Fine fuels and ground vegetation were consumed but the low intensity fire only scorched the understorey trees, which subsequently died and left the Eucalyptus obliqua canopy largely intact. In the aftermath of the fire, there was prolific seedling regeneration, a sustained reduction in leaf area index, and the forest switched from being a carbon sink before the fire to becoming a carbon source during the first post-fire growing season.
Acceptance of a Protein Concentrate from Alfalfa (Medicago sativa) by Yellow Perch (Perca flavescens) Fed a Formulated Diet
Authors: Coburn Jessica , Wells M. Scott , Phelps Nicholas B. D. , Gaylord T. Gibson , Samac Deborah A.
Published: March 2021

The majority of plant proteins used in aquatic feeds are derived from seed meals, which may contain antinutritional factors. Protein concentrates from plant foliage have received less attention in fish feeding trials. Alfalfa protein concentrate (APC) is derived from fresh alfalfa foliage that contains approximately 52% protein and is low in fiber. A feeding trial was done to assess growth and feed efficiency responses of yellow perch (Perca flavescens) fed a formulated diet with 180 g/kg APC replacing all fishmeal compared to a control isonitrogenous diet with fishmeal. Yellow perch accepted the APC diet but gained weight at a lower specific growth rate (−0.07% per day) and had an elevated feed conversion ratio (+0.32 g feed/g growth) than fish on the control diet containing fishmeal. There was no impact on survivorship or condition nor differences in fillet yield or composition in fish on the diet with APC compared to the control fishmeal diet. These findings indicate that although replacing fishmeal with APC in a perch diet resulted in slower growth rates, the APC was accepted and has promise as a sustainable protein in aquatic feeds.
Influence of Different Prebiotics on Viability of Lactobacillus casei, Lactobacillus plantarum and Lactobacillus rhamnosus Encapsulated in Alginate Microcapsules
Authors: Luca Liliana , Oroian Mircea
Published: March 2021

As the production and maintenance of a sufficient number of microencapsulated probiotics is still a test for the food industry, the present study addressed the testing of three prebiotics: chicory inulin, soluble potato starch, oligofructose and a control carbon source, namely glucose, as a component part of the encapsulation matrix. Using the extrusion encapsulation technique, it was possible to obtain microcapsules whose matrix composition and dimensions correspond to the requirements of the food industry. The microcapsules obtained showed significantly different physicochemical properties, with different survival rates during processing, storage and in simulated gastrointestinal conditions. The encapsulation efficiency was very high in relation to the dimensions of the microcapsules and the technique used (between 87.00–88.19%). The microcapsules obtained offered a very good viability (between 8.30 ± 0.00–9.00 ± 0, 02 log10 cfu/g) during the 30 days of storage at 2–8 degrees and also in the simulated gastrointestinal conditions (between 7.98–8.22 log10 cfu/g). After 30 days, the lowest viability was registered in the microcapsules with glucose 6.78 ± 0.15 log10 cfu/g. It was found that after 4 h of action of gastrointestinal juices on the microcapsules stored for 30 days, cell viability falls within the limits recommended by the Food and Agriculture Organization of the United Nations (FAO) (106–107 CFU/mL or g of food. This study demonstrated that using prebiotic encapsulation matrix increases cell viability and protection and that the extrusion encapsulation method can be used in the production of probiotic microcapsules for the food industry.
A Possible Quasi-Periodic Oscillation in the X-ray Emission of 3C 120
Authors: Agarwal Aditi , Rani Priyanka , Prince Raj , Stalin C. S. , Anupama G. C. , Agrawal Vipul
Published: March 2021

We present here the detection of a possible quasi-periodic oscillation (QPO) signal in the X-ray light curve of the active galactic nucleus 3C 120, a broad line radio galaxy at z = 0.033. The hint of a QPO at the 3σ level at 7.1 × 10−6 Hz (∼1.65 days) was detected based on the analysis of X-ray data acquired in the 3–79 keV band by the Nuclear Spectroscopic Telescope Array (NuSTAR). The data, when processed separately in the soft (3–10 keV), hard (10–79 keV) and the total (3–79 keV) bands using four different techniques, namely discrete correlation function, Lomb Scargle periodogram, structure–function, and power spectral density indicated the presence of a QPO. 3C 120 very well fits in the negative correlation in the frequency of the QPO versus the black hole mass (FQPO versus MBH) diagram known for stellar-mass and supermassive black hole sources. Considering the observed signs of QPO to represent the innermost stable orbit of the accretion disk, we found a black hole mass of 1.9×109 M⊙ for a Kerr black hole and 3.04×108 M⊙ for a Schwarzschild black hole. This deduced black hole mass from QPO measurement is a few times larger than the black hole mass obtained from reverberation mapping observations.
Joseph Naytowhow: waniskâ “Wake up!” to Wholeness through nêhiyawîhtwâwin
Authors: Naytowhow Joseph , Kephart Elise
Published: March 2021

In this article, the authors present the teachings of nêhiyaw (Cree) Emerging Elder and Knowledge Keeper Joseph Naytowhow. In a celebrated nêhiyaw (Cree) fashion, storytelling and language are used as examples of a non-linear and sometimes complicated journey back to self, culture, nature and healing. Against the background of being kidnapped, imprisoned in a religious institution, and robbed of all-things nêhiyaw (Cree), this article offers a sense of Joseph Naytowhow’s journey back to intimacy, love, and affection which aids in one’s search for emotional safety. Joseph utilizes nêhiyawîhtwâwin (Cree worldview and culture) knowledge tools such as dreaming to aid in his journey back to nêhiyawîhtwâwin (Cree culture) and nêhiyawêwin (Cree language). From a residential school internee to a leader and emerging Elder, he notes the importance of mentors in a relational approach to healing. This article provides an invitation through “the sunrise song” to “Wake up!” and create a more respectful and reciprocal world of internal wholeness.
Continence Status and Presence of Pressure Skin Injury among Special Elderly Nursing Home Residents in Japan: A Nationwide Cross-Sectional Survey
Authors: Suzuki Motofumi , Kodaira Megumi , Suyama Keiko , Murata Taro , Kume Haruki
Published: March 2021

Urinary and fecal incontinence as well as skin pressure injury are common healthcare problems in nursing homes; however, the prevalence and related risk factors were not well understood in the Japanese special elderly nursing home settings. We surveyed the prevalence of urinary, fecal and double incontinence, and skin pressure injury among the elderly living in special elderly nursing homes in Japan. A nationwide cross-sectional epidemiological survey was conducted with a total of 4881 residents. The prevalence of urinary, fecal and double incontinence was 82.9%, 68.9% and 64.9%, respectively. Skin pressure injury was found in 283 residents (283/4881, 5.8%). Age, Care-Needs level, loss of voiding desire, and fecal incontinence were significant risk factors for urinary incontinence. Residential period, Care-Needs level, loss of voiding and defecation desires, and urinary incontinence were significant risk factors for fecal incontinence. Only male sex was a significant risk factor for skin pressure injury. Our study revealed continence status and the prevalence of pressure skin injury among older adult residents who receive end-of-life care in special elderly nursing homes in Japan. Further studies should be conducted to examine whether recovery of urinary and fecal sensations improves continence status.
Close Association between Awareness of Teeth-Alignment Disorder and Systemic Disorders in Late Adolescence
Authors: Abe Masanobu , Mitani Akihisa , Yao Atsushi , Zhang Chun-Dong , Hoshi Kazuto , Yanagimoto Shintaro
Published: March 2021

Background: Oral diseases are associated with various systemic disorders. Our previous research revealed new insights into the close relationship between occlusal disorder (functional disorder) and systemic disorders (allergic rhinitis, asthma, and arrhythmia) in late adolescence. Here, we investigated whether there was an association between the awareness of teeth-alignment disorder (morphological disorder) and common systemic disorders. Subjects and Methods: We retrospectively reviewed the data of the mandatory medical questionnaire that is required for the freshman medical checkup in Japan. We collected the data of all students who completed the questionnaire between April 2017 and April 2019. The data were analyzed using the χ2 test, and a multivariate analysis was performed with a binomial logistic regression model. Results: The subjects were 8903 students aged 17–19 who had no awareness of occlusal disorder. The rate of awareness of teeth-alignment disorder was 20.43% (1819 of 8903 eligible subjects), and the aware students had significantly greater rates of gum bleeding (p < 0.001), pollinosis (n = 0.007), and atopic dermatitis (n = 0.042). The multivariate analysis revealed significant rates of gum bleeding (odds ratio (OR) 1.540, 95% confidence interval (CI): 1.386–1.711, p < 0.001), pollinosis (OR 1.197, 95% CI: 1.040–1.378, p = 0.012), and female gender (OR 1.141, 95% CI: 1.002–1.299, p = 0.046) among the students with awareness of teeth-alignment disorder. Conclusion: We identified close associations between the awareness of teeth-alignment disorder and both gum bleeding and pollinosis in a late-adolescent population. The systemic disorders that are targeted by teeth-alignment disorder were found to be different from those targeted by occlusal disorder.