bluuebunny/crossref_metadata_2025 huggingface dataset:
Response and Regulatory Network Analysis of Roots and Stems to Abiotic Stress in Populus trichocarpa
Authors: Tao Ran , Liu Yaqiu , Jing Weipeng
Published: August 2022

Abiotic stress is one of the environmental conditions that effects plant survival and growth. As a vital model plant and papermaking raw material, it is very important to identify the differentially expressed genes of Populus trichocarpa Torr. & A.Gray ex Hook under abiotic stress in order to cultivate stress-tolerant plants. We analyzed the whole transcription spectrum and potential differentially expressed genes of 54 groups of roots and stem-xylem sequencing sample data under cold, drought, heat and salt stress for different durations. Gene Ontology (GO) enrichment analysis showed that molecular function played a stronger role in stems’ response to abiotic stress, and genes in roots could barely respond to both cold stress and heat stress. Degree and betweenness centrality were used to identify transcription factors. It was considered that intermediate centrality is more suitable to determine whether the transcription factor is a hub gene. DNA binding was the biggest enrichment, while transcription factors responded to the abiotic stress. The multipronged approach identified in the roots and stems provides a genetic basis for resistance and more targeted genetic improvement of Populus trichocarpa. The comparison of two centralities can more effectively analyze the importance of complex gene network nodes in plants under corresponding abiotic stresses.
Widely Targeted Metabolomics Profiling Reveals the Effect of Powdery Mildew on Wine Grape Varieties with Different Levels of Tolerance to the Disease
Authors: Yu Huan , Li Hongyan , Wei Rongfu , Cheng Guo , Zhou Yongmei , Liu Jinbiao , Xie Taili , Guo Rongrong , Zhou Sihong
Published: August 2022

Powdery mildew is an economic threat for viticulture because it not only affects grape yield, but also causes a series of impacts on the qualities of fruit and wine, especially the flavors and various metabolites. Different grape varieties may have different levels of powdery mildew resistance/tolerance and their components of their metabolome are also various. In this study, two wine grape varieties, Guipu No.6 (GP6) and Marselan (Mar) with different levels of powdery mildew tolerance, were used to compare the quality differences in metabolism level by using the widely targeted metabolomics method. The results show that GP6 has a better powdery mildew leaf tolerance than Mar. A total of 774 metabolites were detected by using a UPLC-QQQ-MS-based metabolomics approach, and 57 differential metabolites were identified as key metabolites that were accumulated after infection with powdery mildew in GP6 and Mar, including phenolic acids, flavonoids, terpenoids, stilbenes, lipids, nucleotides and derivatives, lignans and coumarins, and quinones. This finding indicates that the defense mechanisms of grape fruit are mainly associated with phenylpropane-flavonoid metabolism. Specifically, stilbenes had greater variations after powdery mildew infection in GP6; while in Mar, the variations of flavonoids, especially kaempferol-3-O-glucuronide and luteolin-7-O-glucuronide, were more remarkable. The above results demonstrate that stilbenes may play a more important role than flavonoids in resisting powdery mildew infection in GP6’s fruits, and the drastic variations of these phenolic compounds in different wine grapes after powdery mildew infection might also lead to quality difference in the flavors. This study can provide new insights into the understanding of the cause of powdery mildew tolerance in different grape varieties and the effects on the quality of wine grapes infected with the disease exerted by metabolism level.
Improving the Specific Activity and Thermostability of Psychrophilic Xylosidase AX543 by Comparative Mutagenesis
Authors: Pan Kungang , Liu Zhongqi , Zhang Zhengjie , Jin Shanzheng , Yu Zhao , Liu Tianhui , Zhang Tongcun , Zhao Junqi , Li Zhongyuan
Published: August 2022

Improving the specific activity and thermostability of psychrophilic xylosidase is important for improving its enzymatic performance and promoting its industrial application. Herein, a psychrophilic xylosidase AX543 exhibited activity in the temperature range between 0 and 35 °C, with optimum activity at 20 °C, which is lower than that of other reported psychrophilic xylosidases. The thermostability, specific activity, and catalytic efficiency of the site-directed variants G110S, Q201R, and L2 were significantly enhanced, without affecting the optimal reaction temperature. Comparative protein structural analysis and molecular dynamics simulation indicated that these improvements might be the result of the increased hydrogen bonds interaction and improved structural rigidity. Furthermore, homologous module substitution with four segments demonstrated that the psychrophilic characteristics of AX543 are the results of the whole protein structure, and the C-terminal segment A4 appears to be more essential in determining psychrophilic characteristics, exhibiting potentiality to produce more psychrophilic xylosidases. This study provides valuable structural information on psychrophilic xylosidases and also offers attractive modification strategies to modify catalytic activity, thermostability, and optimal reaction temperature.
Trends of Medical Service Utilization for Tinnitus: Analysis Using 2010–2018 Health Insurance Review and Assessment Service National Patient Sample Data
Authors: Min Taewoon , Yeo Jiyoon , Lee Ye-Seul , Kim Song-Yi , Lee Donghyo , Ha In-Hyuk
Published: August 2022

Given the increasing prevalence of tinnitus and expenditure related to its treatment, it is important to identify the efficacy of different treatment methods used for its diagnosis and treatment. To this end, this study analyzed the trends of medical service utilization for tinnitus in adult patients from 2010 to 2018 based on a national sample of medical claims data from the Health Insurance Review and Assessment Service National Patient Samples database. A total of 94,323 patients with tinnitus were identified in Korea between 2010 and 2018. The results confirmed that the number of patients, claim numbers, and expenditures steadily increased during the nine-year period. Blood circulation agents were the most commonly used drug therapy; however, the frequency of their use gradually decreased, whereas that of tinnitus and vertigo medicines gradually increased. Total and average expenditure per patient nearly doubled in this period. The study showed that medication trends are changing from blood circulation agents to tinnitus or vertigo medicines. The findings of this study may be helpful for clinicians and researchers in the study, treatment, and management of tinnitus.
Vascular Implications of COVID-19: Role of Radiological Imaging, Artificial Intelligence, and Tissue Characterization: A Special Report
Authors: Khanna Narendra N. , Maindarkar Mahesh , Puvvula Anudeep , Paul Sudip , Bhagawati Mrinalini , Ahluwalia Puneet , Ruzsa Zoltan , Sharma Aditya , Munjral Smiksha , Kolluri Raghu , Krishnan Padukone R. , Singh Inder M. , Laird John R. , Fatemi Mostafa , Alizad Azra , Dhanjil Surinder K. , Saba Luca , Balestrieri Antonella , Faa Gavino , Paraskevas Kosmas I. , Misra Durga Prasanna , Agarwal Vikas , Sharma Aman , Teji Jagjit , Al-Maini Mustafa , Nicolaides Andrew , Rathore Vijay , Naidu Subbaram , Liblik Kiera , Johri Amer M. , Turk Monika , Sobel David W. , Pareek Gyan , Miner Martin , Viskovic Klaudija , Tsoulfas George , Protogerou Athanasios D. , Mavrogeni Sophie , Kitas George D. , Fouda Mostafa M. , Kalra Manudeep K. , Suri Jasjit S.
Published: August 2022

The SARS-CoV-2 virus has caused a pandemic, infecting nearly 80 million people worldwide, with mortality exceeding six million. The average survival span is just 14 days from the time the symptoms become aggressive. The present study delineates the deep-driven vascular damage in the pulmonary, renal, coronary, and carotid vessels due to SARS-CoV-2. This special report addresses an important gap in the literature in understanding (i) the pathophysiology of vascular damage and the role of medical imaging in the visualization of the damage caused by SARS-CoV-2, and (ii) further understanding the severity of COVID-19 using artificial intelligence (AI)-based tissue characterization (TC). PRISMA was used to select 296 studies for AI-based TC. Radiological imaging techniques such as magnetic resonance imaging (MRI), computed tomography (CT), and ultrasound were selected for imaging of the vasculature infected by COVID-19. Four kinds of hypotheses are presented for showing the vascular damage in radiological images due to COVID-19. Three kinds of AI models, namely, machine learning, deep learning, and transfer learning, are used for TC. Further, the study presents recommendations for improving AI-based architectures for vascular studies. We conclude that the process of vascular damage due to COVID-19 has similarities across vessel types, even though it results in multi-organ dysfunction. Although the mortality rate is ~2% of those infected, the long-term effect of COVID-19 needs monitoring to avoid deaths. AI seems to be penetrating the health care industry at warp speed, and we expect to see an emerging role in patient care, reduce the mortality and morbidity rate.
3D Approaches in Complex CHD: Where Are We? Funny Printing and Beautiful Images, or a Useful Tool?
Authors: Spanaki Adriani , Kabir Saleha , Stephenson Natasha , van Poppel Milou P. M. , Benetti Valentina , Simpson John
Published: August 2022

Echocardiography, CT and MRI have a crucial role in the management of congenital heart disease (CHD) patients. All of these modalities can be presented in a 2D or a 3D rendered format. The aim of this paper is to review the key advantages and potential limitations, as well as the future challenges of a 3D approach in each imaging modality. The focus of this review is on anatomic rather than functional assessment. Conventional 2D echocardiography presents limitations when imaging complex lesions, whereas 3D imaging depicts the anatomy in all dimensions. CT and MRI can visualise extracardiac vasculature and guide complex biventricular repair. Three-dimensional printed models can be used in depicting complex intracardiac relationships and defining the surgical strategy in specific lesions. Extended reality imaging retained dynamic cardiac motion holds great potential for planning surgical and catheter procedures. Overall, the use of 3D imaging has resulted in a better understanding of anatomy, with a direct impact on the surgical and catheter approach, particularly in more complex cases.
Waste Heat Source Profiles for Marine Application of Organic Rankine Cycle
Authors: Ng Chunwee , Tam Ivan C. K. , Wetenhall Ben
Published: August 2022

The maritime industry will continue to see increasing regulatory requirements to reduce carbon emissions from ships’ operations. Improving the energy efficiency of ships with waste heat recovery systems based on the organic Rankine cycle (ORC) is an attractive way to meet these tightening requirements. The operational profile of a ship has a huge influence on the feasibility of installing ORC onboard as it affects the waste heat source profile from the diesel engines. However, to date, scant attention has been paid to examining the effects that the operational profile has on the marine application of ORC as it is both difficult and expensive to obtain. The present paper aims to describe a methodology that can overcome this problem by developing a generic ship speed profile that defines the ship’s operational profile. This speed profile works together with a fit-for-purpose diesel engine waste heat model to derive a waste heat source profile that is used as the input to a thermoeconomic analysis that can justify the installation of ORC. The proposed methodology allows for an objective comparison of the feasibility of ORC subjected to variations in the operational profile. Furthermore, the optimum ORC design can be identified to meet payback time expectations of different shipowners.
The Sorting Nexin Genes ChSNX4 and ChSNX41 Are Required for Reproductive Development, Stress Adaption and Virulence in Cochliobolus heterostrophus
Authors: Yu Huilin , Jia Wantong , Li Zhongxiang , Gao Chaofeng , Pan Hongyu , Zhang Xianghui
Published: August 2022

Sorting nexins are a conserved protein family involved in many cellular processes in fungi, and the function of sorting nexin Snx4 (Atg24) and Snx41 (Atg20) in Cochliobolus heterostrophus was not clear. The ΔChsnx4 and ΔChsnx41 mutants were generated by a PCR-based marker method to determine the roles of Snx4 and Snx41 in reproductive development, stress adaption, and virulence in C. heterostrophus. Compared with the wild-type strain, the ΔChsnx4 and ΔChsnx41 mutants exhibited obvious changes in vegetative growth and in morphology of conidia. In addition, the conidiation, appressorium formation, and virulence of snx4 and snx41 mutants were dramatically reduced. Moreover, ΔChsnx4 and ΔChsnx41 mutants were more sensitive to oxidative stress (menadione and H2O2), cell wall integrity stress (Congo red and calcofluor white), fungicides, and isothiocyanates. All the phenotypes mentioned above were restored in complemented strains. In addition, ChSnx4 and ChSnx41 were proven to interact with each other through yeast two-hybrid. Taken together, these findings indicated that ChSNX4 and ChSNX41 were important for fungal growth, asexual development, stress adaption, and virulence in C. heterostrophus.
Synthesis of 4-(tert-Butyldimethylsilyl)-4,5,6,7-tetrahydro-[1,2,3]triazolo[1,5-a]pyridine
Authors: Kroņkalne Rasma , Beļaunieks Rūdolfs , Turks Māris
Published: August 2022

SN2 rection between 4-(tert-butyldimethylsilyl)hex-5-yn-1-yl 4-methylbenzenesulfonate and NaN3 in DMF at 80 °C provided (6-azidohex-1-yn-3-yl)(tert-butyl)dimethylsilane intermediate, which underwent in situ intramolecular thermal Huisgen azide–alkyne cycloaddition reaction. This one-pot process gave 4-(tert-butyldimethylsilyl)-4,5,6,7-tetrahydro-[1,2,3]triazolo[1,5-a]pyridine in 78% yield.
Specific Features of Operation of Distributed Generation Facilities Based on Gas Reciprocating Units in Internal Power Systems of Industrial Entities
Authors: Ilyushin Pavel , Filippov Sergey , Kulikov Aleksandr , Suslov Konstantin , Karamov Dmitriy
Published: August 2022

The creation of a decentralized low-carbon energy infrastructure is the main trend in the development of the electric power industry in many countries. Distributed generation facilities (DGs) based on gas reciprocating units (GRUs) are often built by industrial entities for the efficient utilization of secondary energy resources in order to minimize the environmental impact. Modern GRUs have some advantages, but they have design features that should be factored in when connecting them to the internal power systems of industrial entities. Incorrect consideration of possible operating conditions of GRU in their design can lead to their damage, excessive shutdowns, and disruptions in power supply to essential power consumers with significant damage and losses from undersupply of their products. Excessive shutdowns of GRUs are often caused by a non-selective choice of settings for relay protection devices or by load surges that exceed the allowable ones. With high availability factors, GRUs are disconnected five to eight times more often compared to large gas turbine and steam turbine power units. The large total power consumed by electric motors, as part of the load of an industrial entity, determines the nature and parameters of electromechanical transient processes during emergency disturbances. The presented analysis of issues facing real DG facilities relies on the acts of investigation into the causes of accidents. Calculations have shown that the action of the “Load Agreement Module” in the GRU excitation controller can provoke the occurrence of a voltage avalanche in the internal power system with a complete shutdown of the load. The paper presents recommendations on the choice of control algorithms and voltage settings for the GRU excitation controller. Technical solutions are given to prevent damage and excessive shutdowns of GRU in various operating conditions of the system, and to help ensure a reliable power supply to power consumers. The change in approaches to the design of DG facilities is substantiated in the light of their significant differences from other electric power facilities.
Deep Learning for Vessel Trajectory Prediction Using Clustered AIS Data
Authors: Yang Cheng-Hong , Lin Guan-Cheng , Wu Chih-Hsien , Liu Yen-Hsien , Wang Yi-Chuan , Chen Kuo-Chang
Published: August 2022

Accurate vessel track prediction is key for maritime traffic control and management. Accurate prediction results can enable collision avoidance, in addition to being suitable for planning routes in advance, shortening the sailing distance, and improving navigation efficiency. Vessel track prediction using automatic identification system (AIS) data has attracted extensive attention in the maritime traffic community. In this study, a combining density-based spatial clustering of applications with noise (DBSCAN)-based long short-term memory (LSTM) model (denoted as DLSTM) was developed for vessel prediction. DBSCAN was used to cluster vessel tracks, and LSTM was then used for training and prediction. The performance of the DLSTM model was compared with that of support vector regression, recurrent neural network, and conventional LSTM models. The results revealed that the proposed DLSTM model outperformed these models by approximately 2–8%. The proposed model is able to provide a better prediction performance of vessel tracks, which can subsequently improve the efficiency and safety of maritime traffic control.
Chemical and Physical Ionic Liquids in CO2 Capture System Using Membrane Vacuum Regeneration
Authors: Vadillo José Manuel , Díaz-Sainz Guillermo , Gómez-Coma Lucía , Garea Aurora , Irabien Angel
Published: August 2022

Carbon Capture Utilization and Storage technologies are essential mitigation options to reach net-zero CO2 emissions. However, this challenge requires the development of sustainable and economic separation technologies. This work presents a novel CO2 capture technology strategy based on non-dispersive CO2 absorption and membrane vacuum regeneration (MVR) technology, and employs two imidazolium ionic liquids (ILs), [emim][Ac] and [emim][MS], with different behavior to absorb CO2. Continuous absorption–desorption experiments were carried out using polypropylene hollow fiber membrane contactors. The results show the highest desorption behavior in the case of [emim][Ac], with a MVR performance efficiency of 92% at 313 K and vacuum pressure of 0.04 bar. On the other hand, the IL [emim][MS] reached an efficiency of 83% under the same conditions. The MVR technology could increase the overall CO2 capture performance by up to 61% for [emim][Ac] and 21% for [emim][MS], which represents an increase of 26% and 9%, respectively. Moreover, adding 30%vol. demonstrates that the process was only favorable by using the physical IL. The results presented here indicate the interest in membrane vacuum regeneration technology based on chemical ILs, but further techno-economic evaluation is needed to ensure the competitiveness of this novel CO2 desorption approach for large-scale application.
Host–Gut Microbiome Metabolic Interactions in PFAS-Impacted Freshwater Turtles (Emydura macquarii macquarii)
Authors: Beale David J. , Nguyen Thao V. , Shah Rohan M. , Bissett Andrew , Nahar Akhikun , Smith Matthew , Gonzalez-Astudillo Viviana , Braun Christoph , Baddiley Brenda , Vardy Suzanne
Published: August 2022

Per-and polyfluoroalkyl substances (PFAS) are a growing concern for humans, wildlife, and more broadly, ecosystem health. Previously, we characterised the microbial and biochemical impact of elevated PFAS on the gut microbiome of freshwater turtles (Emydura macquarii macquarii) within a contaminated catchment in Queensland, Australia. However, the understanding of PFAS impacts on this species and other aquatic organisms is still very limited, especially at the host–gut microbiome molecular interaction level. To this end, the present study aimed to apply these leading-edge omics technologies within an integrated framework that provides biological insight into the host turtle–turtle gut microbiome interactions of PFAS-impacted wild-caught freshwater turtles. For this purpose, faecal samples from PFAS-impacted turtles (n = 5) and suitable PFAS-free reference turtles (n = 5) were collected and analysed. Data from 16S rRNA gene amplicon sequencing and metabolomic profiling of the turtle faeces were integrated using MetOrigin to assign host, microbiome, and co-metabolism activities. Significant variation in microbial composition was observed between the two turtle groups. The PFAS-impacted turtles showed a higher relative abundance of Firmicutes and a lower relative abundance of Bacteroidota than the reference turtles. The faecal metabolome showed several metabolites and pathways significantly affected by PFAS exposure. Turtles exposed to PFAS displayed altered amino acid and butanoate metabolisms, as well as altered purine and pyrimidine metabolism. It is predicted from this study that PFAS-impacted both the metabolism of the host turtle and its gut microbiota which in turn has the potential to influence the host’s physiology and health.
Cesium Lead Iodide Perovskites: Optically Active Crystal Phase Stability to Surface Engineering
Authors: Wang Yixi , Zhao Hairong , Piotrowski Marek , Han Xiao , Ge Zhongsheng , Dong Lizhuang , Wang Chengjie , Pinisetty Sowjanya Krishna , Balguri Praveen Kumar , Bandela Anil Kumar , Thumu Udayabhaskararao
Published: August 2022

Among perovskites, the research on cesium lead iodides (CsPbI3) has attracted a large research community, owing to their all-inorganic nature and promising solar cell performance. Typically, the CsPbI3 solar cell devices are prepared at various heterojunctions, and working at fluctuating temperatures raises questions on the material stability-related performance of such devices. The fundamental studies reveal that their poor stability is due to a lower side deviation from Goldschmidt’s tolerance factor, causing weak chemical interactions within the crystal lattice. In the case of organic–inorganic hybrid perovskites, where their stability is related to the inherent chemical nature of the organic cations, which cannot be manipulated to improve the stability drastically whereas the stability of CsPbI3 is related to surface and lattice engineering. Thus, the challenges posed by CsPbI3 could be overcome by engineering the surface and inside the CsPbI3 crystal lattice. A few solutions have been proposed, including controlled crystal sizes, surface modifications, and lattice engineering. Various research groups have been working on these aspects and had accumulated a rich understanding of these materials. In this review, at first, we survey the fundamental aspects of CsPbI3 polymorphs structure, highlighting the superiority of CsPbI3 over other halide systems, stability, the factors (temperature, polarity, and size influence) leading to their phase transformations, and electronic band structure along with the important property of the defect tolerance nature. Fortunately, the factors stabilizing the most effective phases are achieved through a size reduction and the efficient surface passivation on the delicate CsPbI3 nanocrystal surfaces. In the following section, we have provided the up-to-date surface passivating methods to suppress the non-radiative process for near-unity photoluminescence quantum yield, while maintaining their optically active phases, especially through molecular links (ligands, polymers, zwitterions, polymers) and inorganic halides. We have also provided recent advances to the efficient synthetic protocols for optically active CsPbI3 NC phases to use readily for solar cell applications. The nanocrystal purification techniques are challenging and had a significant effect on the device performances. In part, we summarized the CsPbI3-related solar cell device performances with respect to the device fabrication methods. At the end, we provide a brief outlook on the view of surface and lattice engineering in CsPbI3 NCs for advancing the enhanced stability which is crucial for superior optical and light applications.
Silicon-on-Insulator Optical Waveguide Pressure Sensor Based on Mach-Zehnder Interferometer
Authors: Li Chen , Zhang Chi , Yang Lijun , Guo Fangtong
Published: August 2022

At present, there are few methods to measure optical pressure using MEMS. However, due to its high precision and fast response, a ridge waveguide pressure sensor based on a Mach–Zehnder interferometer is designed in this paper. Through the design and optimization of each component of the structure, the sensitivity of the pressure sensor was 2.2 × 10−3 W/kPa and the linearity was 5.9 × 10−3. The sensor had a good performance and small volume, which can be used in the field of light pressure measurement and other fields that required the measurement small pressures, such as the biomedicine field.
Evaluation of Various Lactic Acid Bacteria and Generic E. coli as Potential Nonpathogenic Surrogates for In-Plant Validation of Biltong Dried Beef Processing
Authors: Karolenko Caitlin E. , Wilkinson Jade , Muriana Peter M.
Published: August 2022

Validation studies conducted within a food processing facility using surrogate organisms could better represent the manufacturing process than controlled laboratory studies with pathogenic bacteria on precision equipment in a BSL-2 lab. The objectives of this project were to examine potential surrogate bacteria during biltong processing, conduct biltong surrogate validation lethality studies, and measure critical factors and intrinsic parameters during processing. Beef pieces (1.9 cm × 5.1 cm × 7.6 cm) were inoculated with four-strain mixtures of Carnobacterium divergens/C. gallinarum, Pediococcus acidilactici/P. pentosaceous, and Biotype 1 E. coli ATCC BAA (-1427, -1428, -1429, and -1430), as well as a two-strain mixture of Latilactobacillus sakei and other commercially available individual bacterial cultures (P. acidilactici Saga200/Kerry Foods; Enterococcus faecium 201224-016/Vivolac Cultures). Inoculated beef was vacuum-tumbled in marinade and dried in a humidity-controlled oven for 8–10 days (24.9 °C; 55% relative humidity). Microbial enumeration of surviving surrogate bacteria and evaluation of intrinsic factors (water activity, pH, and salt concentration) were performed post inoculation, post marination, and after 2, 4, 6, 8, and 10 days of drying. Trials were performed in duplicate replication with triplicate samples per sampling time and analyzed by one-way RM-ANOVA. Trials conducted with E. faecium, Pediococcus spp., and L. sakei never demonstrated more than 2 log reduction during the biltong process. However, Carnobacterium achieved a >5 log (5.85 log) reduction over a drying period of 8 days and aligned with the reductions observed in previous trials with pathogenic bacteria (Salmonella, E. coli O157:H7, L. monocytogenes, and S. aureus) in biltong validation studies. Studies comparing resuspended freeze-dried or frozen cells vs. freshly grown cells for beef inoculation showed no significant differences during biltong processing. Carnobacterium spp. would be an effective nonpathogenic in-plant surrogate to monitor microbial safety that mimics the response of pathogenic bacteria to validate biltong processing within a manufacturer’s own facility.
Perfect Photon Indistinguishability from a Set of Dissipative Quantum Emitters
Authors: Guimbao Joaquin , Sanchis Lorenzo , Weituschat Lukas M. , Llorens Jose M. , Postigo Pablo A.
Published: August 2022

Single photon sources (SPS) based on semiconductor quantum dot (QD) platforms are restricted to low temperature (T) operation due to the presence of strong dephasing processes. Although the integration of QD in optical cavities provides an enhancement of its emission properties, the technical requirements for maintaining high indistinguishability (I) at high T are still beyond the state of the art. Recently, new theoretical approaches have shown promising results by implementing two-dipole-coupled-emitter systems. Here, we propose a platform based on an optimized five-dipole-coupled-emitter system coupled to a cavity which enables perfect I at high T. Within our scheme the realization of perfect I single photon emission with dissipative QDs is possible using well established photonic platforms. For the optimization procedure we have developed a novel machine-learning approach which provides a significant computational-time reduction for high demanding optimization algorithms. Our strategy opens up interesting possibilities for the optimization of different photonic structures for quantum information applications, such as the reduction of quantum decoherence in clusters of coupled two-level quantum systems.
Sugar Content and Warning Criteria Evaluation for Popular Sugar-Sweetened Beverages in Taipei, Taiwan
Authors: Yen Chieh , Huang Ya-Li , Chung Mei , Chen Yi-Chun
Published: August 2022

Sugar intake may increase the risk of obesity, cardiovascular disease, diabetes, and dental caries. In Taiwan, people frequently consume sugar-sweetened beverages (SSBs). This study explored the energy and sugar content of Taiwanese SSBs and evaluated them using the Chilean warning label system (>70 kcal/100 mL and >5 g sugar/100 mL) and the World Health Organization (WHO) sugar guideline (≤25 g sugar). A total of 341 SSBs with volumes ≤600 mL were analyzed. No significant differences were observed in sugar per serving among different types of SSBs, but a great variation in portion size (i.e., package size for individual consumption) was noted. The energy and sugar ratios per serving were lower in soft drinks and coffee and tea containing >1 serving than in those containing only one serving. The calorie and sugar ratios per portion were higher in all types of SSBs containing >1 serving per portion than in those containing exactly one serving. Approximately 70.0% of Taiwanese SSBs were classified as high sugar according to the Chilean criteria, and 41.6% of SSBs exceeded the WHO guideline. Moreover, 40.8% of SSBs that were not considered as high sugar according to the Chilean criteria contained >25 g sugar per portion. For individual consumption, it is more clear that nutrition labeling is based on portion rather than serving. Evaluating SSBs on sugar/portion rather than sugar/100 mL will help consumers make better choices.
In Vitro and In Silico Potential Inhibitory Effects of New Biflavonoids from Ochna rhizomatosa on HIV-1 Integrase and Plasmodium falciparum
Authors: Messi Angélique Nicolas , Bonnet Susan Lucia , Owona Brice Ayissi , Wilhelm Anke , Kamto Eutrophe Le Doux , Ndongo Joseph Thierry , Siwe-Noundou Xavier , Poka Madan , Demana Patrick H. , Krause Rui W. M. , Ngo Mbing Joséphine , Pegnyemb Dieudonné Emmanuel , Bochet Christian G.
Published: August 2022

The aim of this study was to identify bioactive secondary metabolites from Ochna rhizomatosa with potential inhibitory effects against HIV and Plasmodium falciparum. A phytochemical study of O. rhizomatosa root barks resulted in the identification of three new biflavonoids (1–3), along with four known ones (4–7). Compound 7 (Gerontoisoflavone A) was a single flavonoid present in the rootbark of the plant and was used as a reference. Compound 1 (IC50 = 0.047 µM) was the only one with a noteworthy inhibitory effect against HIV-1 integrase in vitro. Chicoric acid (IC50 = 0.006 µM), a pure competitive inhibitor of HIV-1 integrase, was used as control. Compound 2 exhibited the highest antiplasmodial activity (IC50 = 4.60 µM) against the chloroquine-sensitive strain of Plasmodium falciparum NF54. Computational molecular docking revealed that compounds 1 and 2 had the highest binding score (−121.8 and −131.88 Kcal/mol, respectively) in comparison to chicoric acid and Dolutegravir (−116 and −100 Kcal/mol, respectively), towards integrase receptor (PDB:3LPT). As far as Plasmodium-6 cysteine s48/45 domain inhibition is concerned, compounds 1 and 2 showed the highest binding scores in comparison to chloroquine, urging the analysis of these compounds in vivo for disease treatment. These results confirm the potential inhibitory effect of compounds 1 and 2 for HIV and malaria treatment. Therefore, our future investigation to find inhibitors of these receptors in vivo could be an effective strategy for developing new drugs.
Activity of Apo-Lactoferrin on Pathogenic Protozoa
Authors: Reyes-López Magda , Ramírez-Rico Gerardo , Serrano-Luna Jesús , de la Garza Mireya
Published: August 2022

Parasites and other eventually pathogenic organisms require the ability to adapt to different environmental conditions inside the host to assure survival. Some host proteins have evolved as defense constituents, such as lactoferrin (Lf), which is part of the innate immune system. Lf in its iron-free form (apo-Lf) and its peptides obtained by cleavage with pepsin are microbicides. Parasites confront Lf in mucosae and blood. In this work, the activity of Lf against pathogenic and opportunistic parasites such as Cryptosporidium spp., Eimeria spp., Entamoeba histolytica, Giardia duodenalis, Leishmania spp., Trypanosoma spp., Plasmodium spp., Babesia spp., Toxoplasma gondii, Trichomonas spp., and the free-living but opportunistic pathogens Naegleria fowleri and Acanthamoeba castellani were reviewed. The major effects of Lf could be the inhibition produced by sequestering the iron needed for their survival and the production of oxygen-free radicals to more complicated mechanisms, such as the activation of macrophages to phagocytes with the posterior death of those parasites. Due to the great interest in Lf in the fight against pathogens, it is necessary to understand the exact mechanisms used by this protein to affect their virulence factors and to kill them.
Perfect Solar Absorber with Extremely Low Infrared Emissivity
Authors: Wang Zhaolong , Wei Yinbao , Liu Zhen , Duan Guihui , Yang Dongsheng , Cheng Ping
Published: August 2022

We propose a multi-materials solar absorber consisting of regularly arranged cross resonators made of Ni, and nanocylinders made of Ti on their four corners. The finite difference time domain (FDTD) method is used to study the absorption performance of solar energy, the inherent absorption mechanisms and influencing factors of the absorber submerged in water. The proposed absorber achieves a nearly perfect absorption in the wavelength range of 300–2400 nm with an average absorptance higher than 98%, indicating that most of solar energy will be harvested. In addition, the high absorptance in the solar spectrum originates from the plasmon resonances of cross resonators and the magnetic polariton from the interaction between cross nanostructures and multi-material nanocylinders. Moreover, our absorbers are insensitive to the polarization of light due to the symmetry of the structures, but sensitive to the incident angle of the light. Most significantly, our absorbers can effectively prevent heat loss via radiation heat transfer by lowering their emissivity in the infrared region. The present study demonstrates a new design strategy for perfect solar absorbers consist of simple nanostructures made from multi-materials, which promise potential applications of solar energy harvesting and solar steam generation for desalination and wastewater treatment.
Influence of Step Casings on the Cavitation Characteristics of Inducers
Authors: Han Huan , Geng Da , Xiang Le , Xu Kaifu , Ren Zibo , Liu Shuhong , Zuo Zhigang
Published: August 2022

Rotating cavitation (RC) in inducers mainly degrades performance with unstable radial force. In this paper, the influence of step casings on cavitation performance and the instabilities of inducers were numerically investigated. Firstly, a numerical scheme was validated by means of a comparison with experimental measurements taken for an original inducer (Model O). We then performed simulations with five different step casing models (Models A–E), in which the starting locations and enlargement sizes of the step casings were altered. The interaction between tip leakage flow and main flow in the inter-blade passages was found to highly affected by cavity structure and blade loading. Considering both cavitation performance and radial force, our results suggest that Model E is an alternative improvement. In short, this study provides a standard workflow for the effective suppression of rotating cavitation in inducers by the simple adoption of the step casing configuration presented here in engineering practice.
Minimizing Foaming and Bulking in Activated Sludge with Bacteriophage Treatment: A Review of Mathematical Modeling
Authors: Vesga-Baron Alejandra , Chamy Rolando , Vande Wouwer Alain
Published: August 2022

The interest in the ability of phages to control bacterial populations has extended from medical applications into the fields of agriculture, aquaculture, and the food industry. In particular, several authors have proposed using bacteriophages as an alternative method to control foaming and bulking in wastewater treatment. This strategy has shown successful results at the laboratory scale. However, this technology is still in development, and there are several challenges to overcome before bacteriophages can be widely used to control foaming and bulking in pilot or larger-scale treatment plants. Several models of the infection mechanisms in individual bacteria–phage pairs have been reported, i.e., for controlled systems with only one bacterium species in the presence of one phage species. However, activated sludge treatment systems largely differ from this situation, which opens a large horizon for future research. Mathematical models will play a key role in this development process, and this review offers an overview of the proposed models: their applications, potential, and challenges. A particular focus is placed on the model properties, such as parameter identifiability and states’ observability, which are essential for process prediction, monitoring, or dynamic optimization.
Integrated Process for Producing Glycolic Acid from Carbon Dioxide Capture Coupling Green Hydrogen
Authors: Wang Dongliang , Li Jingwei , Meng Wenliang , Wang Jian , Wang Ke , Zhou Huairong , Yang Yong , Fan Zongliang , Fan Xueying
Published: August 2022

A novel process path is proposed to produce glycolic acid (GA) from CO2 as the feedstock, including CO2 capture, power-to-hydrogen, CO2 hydrogenation to methanol, methanol oxidation to formaldehyde, and formaldehyde carbonylation units. The bottlenecks are discussed from the perspectives of carbon utilization, CO2 emissions, total site energy integration, and techno-economic analysis. The carbon utilization ratio of the process is 82.5%, and the CO2 capture unit has the largest percentage of discharge in carbon utilization. Among the indirect emissions of each unit, the CO2 hydrogenation to methanol has the largest proportion of indirect carbon emissions, followed by the formaldehyde carbonylation to glycolic acid and the CO2 capture. After total site energy integration, the utility consumption is 1102.89 MW for cold utility, 409.67 MW for heat utility, and 45.98 MW for power. The CO2 hydrogenation to methanol makes the largest contribution to utility consumption due to the multi-stage compression of raw hydrogen and the distillation of crude methanol. The unit production cost is 834.75 $/t-GA; CO2 hydrogenation to methanol accounts for the largest proportion, at 70.8% of the total production cost. The total production cost of the unit depends on the price of hydrogen due to the currently high renewable energy cost. This study focuses on the capture and conversion of CO2 emitted from coal-fired power plants, which provides a path to a feasible low-carbon and clean use of CO2 resources.
Special Issue on “Frontiers in Connecting Steady-State and Dynamic Approaches for Modelling Cell Metabolic Behavior”
Authors: Peres Sabine , Jolicoeur Mario
Published: August 2022

Understanding the behaviour of cell metabolism is the crucial key in bioprocess development and optimization, as well as in the development of efficient therapies [...]
Reptile Search Algorithm (RSA)-Based Selective Harmonic Elimination Technique in Packed E-Cell (PEC-9) Inverter
Authors: Khan Rashid , Sabir Bushra , Sarwar Adil , Liu Hwa-Dong , Lin Chang-Hua
Published: August 2022

The multilevel inverters (MLIs) are capable of handling large quantities of power and generating high-quality output voltages. Consequently, the size of the filters is reduced, and the circuitry is simplified. As a result, they have a diverse range of uses in the industrial sector, especially in smart grids. The input voltage boosting feature is required to utilize the MLI with renewable energy. In addition, a large number of components are required to attain higher output voltage levels, which increases the cost of the circuit and weight. A variety of MLI topologies have been identified to reduce losses, device quantity, and device ratings. The selective harmonic elimination (SHE) approaches reduce distinct lower order harmonics by computing the ideal switching angles. This research presents a nine–level Packed E–Cell (PEC–9) inverter that uses selective harmonic elimination to eliminate total harmonic distortion. In order to calculate the best switching angle, the reptile search algorithm (RSA) is implemented in this paper, a nature–inspired metaheuristic algorithm inspired by the hunting behavior of the crocodile. The hunting behavior of crocodiles is implemented in two main steps: the first is encircling, which is accomplished by belly walking or high walking, and the second is hunting, which is accomplished by hunting cooperation or hunting coordination. In this technique, nonlinear transcendental equations have been solved. The simulation was run in the MATLAB R2021b software environment. The simulation results suggest that the RSA outperforms the other metaheuristic algorithms. Furthermore, the simulation result was validated on a hardware setup using DSP–TMS320F28379D in the laboratory.
Reclaiming the Value of Cotton Waste Textiles: A New Improved Method to Recycle Cotton Waste Textiles via Acid Hydrolysis
Authors: Ruuth Edvin , Sanchis-Sebastiá Miguel , Larsson Per Tomas , Teleman Anita , Jiménez-Quero Amparo , Delestig Sara , Sahlberg Viktor , Salén Patricia , Sanchez Ortiz Marjorie , Vadher Simran , Wallberg Ola
Published: August 2022

The fashion industry is becoming one of the largest emitters worldwide due to its high consumption of raw materials, its effluents, and the fact that every garment will eventually contribute to the vast amount of waste being incinerated or accumulating in landfills. Although fiber-to-fiber recycling processes are being developed, the mechanical properties of the textile fibers are typically degraded with each such recycle. Thus, tertiary recycling alternatives where textiles are depolymerized to convert them into valuable products are needed to provide end-of-life alternatives and to achieve circularity in the fashion industry. We have developed a method whereby cotton waste textiles are depolymerized to form a glucose solution, using sulfuric acid as the sole catalyst, with a high yield (>70%). The glucose solution produced in this process has a high concentration (>100 g/L), which reduces the purification cost and makes the process industrially relevant. This method can be applied regardless of the quality of the fibers and could therefore process other cellulosic fibers such as viscose. The glucose produced could subsequently be fermented into butanediol or caprolactam, precursors for the production of synthetic textile fibers, thus retaining the value of the waste textiles within the textile value chain.
Impact of Snow Cover Phenology on the Vegetation Green-Up Date on the Tibetan Plateau
Authors: Xu Jingyi , Tang Yao , Xu Jiahui , Shu Song , Yu Bailang , Wu Jianping , Huang Yan
Published: August 2022

Variations in snow cover resulting from global warming inevitably affect alpine vegetation growth on the Tibetan Plateau (TP), but our knowledge of such influences is still limited. Here, we investigated the relationship between snow cover and alpine vegetation during 2003–2020 on the TP using the satellite-derived vegetation green-up date (GUD) and metrics of snow cover phenology, namely the snow cover onset date (SCOD), snow cover end date (SCED), snow cover duration (SCD), and snowmelt onset date (SMOD). In this study, we first analyzed the spatiotemporal changes in the GUD and the snow cover phenology metrics on the TP. Pearson’s correlation, gray relation analysis, and linear regression were then employed to determine the impact of snow cover phenology on the GUD. Overall, with the SCOD, SCED, and SMOD delayed by one day, the GUD was advanced by 0.07 and 0.03 days and was postponed by 0.32 days, respectively, and a one-day extension of the SCD resulted in a 0.04-day advance in the GUD. In addition, the roles of vegetation type, topography, and climate factors (temperature and precipitation) in modulating the relationships between snow cover phenology and the GUD were evaluated. The GUD of alpine steppes was negatively correlated with the SCOD and SCED, contrary to that of the other vegetation types. The GUD of alpine steppes was also more sensitive to snow cover phenology than that of other vegetation types. The increase in elevation generally enhanced the sensitivity of the GUD to snow cover phenology. The GUD showed a stronger negative sensitivity to the SCD in warmer areas and a stronger positive sensitivity to the SMOD in wetter areas. Our findings revealed the essential impact of variation in snow cover phenology on the GUD and indicated the complex interference of environmental factors in the relationship between snow cover and vegetation growth.
Evaluation of SNPP and NOAA-20 VIIRS Datasets Using RadCalNet and Landsat 8/OLI Data
Authors: Jing Xin , Uprety Sirish , Liu Tung-Chang , Zhang Bin , Shao Xi
Published: August 2022

In this study, we used RVUS data from RadCalNet as a benchmark to verify the radiometric accuracy and stability of operational and reprocessed SNPP/VIIRS data and the accuracy of NOAA-20/VIIRS data, as well as to assess the efficiency of the SNPP/VIIRS reprocessing algorithm. In addition, to remove the uncertainty of the RVUS site itself, we used Landsat 8/OLI as another benchmark with which to validate the accuracy and stability of VIIRS data through the RUVS site. The radiometric biases of the operational and reprocessed SNPP VIIRS bands were within ±4% and ±2%, respectively, as compared with the RUVS site and OLI, except for the M10 and M11 bands. In particular, the biases of the M5 and M7 bands were reduced by ~2% in this study. NOAA-20 VIIRS, on the other hand, was consistently lower than SNPP by ~2 to ~4% for all the bands. For the equivalent bands, the drift differences between operational and reprocessed SNPP/VIIRS and OLI were no larger than 0.24%/year and 0.1%/year, respectively. The reprocessing algorithm of SNPP VIIRS efficiently improved the radiometric accuracy and stability of the SNPP/VIIRS dataset to meet its specifications.