bluuebunny/crossref_metadata_2025 huggingface dataset:
Automatic MTM-Transcription in Virtual Reality Using the Digital Twin of a Workplace
Authors: Andreopoulos Emmanouil , Gorobets Valentina , Kunz Andreas
Published: October 2022

Despite the high level of automation in industrial production, manual operations still play an important role and contribute significantly to the overall production costs. For the evaluation of these manual processes the ``Methods-Time Measurement'' (MTM) is widely used. This method is applied to real workplaces or mock-ups thereof, while also Virtual Reality (VR) can be used for the representation of such workplaces. However, the evaluation of the workers' performed actions is still done manually, which is a time-consuming and error-prone process. This paper introduces an approach to automatically detect full-body actions of users in VR and consequently derive the appropriate MTM values, without knowledge of a pre-existing workplan. The detection algorithm that was developed is explained in detail and its performance is analyzed through a user study with 30 participants.
EPO regulates neuronal differentiation of adult human neural-crest derived stem cells in a sex-specific manner
Authors: Niemann Tarek , Greiner Johannes F.W. , Kaltschmidt Christian , Kaltschmidt Barbara
Published: October 2022

Background: Sexual differences in the biology of human stem cells are increasingly recognized to influence their proliferation, differentiation and maturation. Especially in neurodegenerative diseases such as Alzheimers disease (AD), Parkinson's disease (PD) or ischemic stroke, sex is a key player for disease progression and recovery of damaged tissue. Recently, the glycoprotein hormone erythropoietin (EPO) has been implicated as a regulator of neuronal differentiation and maturation in female rats.
Methods: In this study, we used adult human neural crest-derived stem cells (NCSCs) as a model system for exploring potential sex specific effects of EPO on human neuronal differentiation. We started with expression validation of the specific EPO receptor (EPOR) by performing PCR analysis in the NCSCs. Next, EPO mediated activation of nuclear factor-κB (NF-κB) via Immunocytochemistry (ICC) was performed, followed by investigating the sex-specific effects of EPO on neuronal differentiation by determining morphological changes in axonal growth and neurite formation accompanied by ICC.
Results: Undifferentiated male and female NCSCs showed a ubiquitous expression of the EPO receptor (EPOR) and EPO treatment resulted in a profound nuclear translocation of NF-kB RELA, a key regulator of neuroprotection and a downstream target of EPO-signaling. Interestingly, nuclear translocation of RELB and c-REL was not induced upon EPO-treatment. Extending the view on the role of sex during human neuronal differentiation, we demonstrate elevated protein amounts of synaptophysin as well as significantly increased axon lengths of female NCSCs-derived neurons upon EPO-treatment compared to their male counterparts.
Conclusion: Our present findings therefore show for the first time an EPO-driven sexual dimorphism in neuronal differentiation of human stem cells and emphasize sex-specific variability as a crucial parameter in stem cell biology and for treating neurodegenerative diseases.
Identification of major quantitative trait loci and candidate genes for seed weight in soybean
Authors: Xu Mengge , Kong Keke , Miao Long , He Jianbo , Liu Tengfei , Zhang Kai , Yue Xiuli , Jin Ting , Gai Junyi , Li Yan
Published: October 2022

Seed weight is an important target of soybean breeding. However, the genes underlying the major quantitative trait loci (QTL) controlling seed weight remain largely unknown. In this study, a soybean population of 300 recombinant inbred lines (RILs) derived from a cross between PI595843 (PI) and WH was used to map the QTL and identify candidate genes for seed weight. The RIL population was genotyped through whole genome resequencing, and phenotyped for 100-seed weight under five environments. A total of 38 QTL were detected, and four major QTL, each explained at least 10% of the variation in 100-seed weight, were identified. Six candidate genes within these four major QTL regions were identified by analyses of their tissue expression patterns, gene annotations, and differential gene expression levels in soybean seeds during four developmental stages between two parental lines. Further sequence variation analyses revealed a C to T substitution in the first exon of the Glyma.19G143300, resulting in an amino acid change between PI and WH, and thus leading to a different predicted kinase domain, which might affect its protein function. Glyma.19G143300 is highly expressed in soybean seeds and encodes a leucine-rich repeat receptor-like protein kinase (LRR-RLK). Its predicted protein has typical domains of LRR-RLK family, and phylogenetic analyses reveled its similarity with the known LRR-RLK protein XIAO (LOC_Os04g48760), which is involved in controlling seed size. The major QTL and candidate genes identified in this study provide useful information for molecular breeding of new soybean cultivars with desirable seed weight.
KLF2 regulates neural differentiation of dental pulp-derived stem cells by modulating autophagy and mitophagy
Authors: Das Hiranmoy , Prateeksha Prateeksha , Naidu Prathyusha , Das Manjusri , Barthels Derek
Published: October 2022

Transplantation of stem cells for treating neurodegenerative disorders is a promising future therapeutic approach. However, the molecular mechanism underlying the neuronal differentiation of mesenchymal stem cells remains inadequately explored. Therefore, the current study aims to define the regulatory role of KLF2 (Kruppel-like factor 2) during the neural differentiation (ND) of dental pulp-derived stem cells (DPSC). Herein, we showed that the expression level of KLF2, autophagy and mitophagy-associated markers were significantly elevated during ND of DPSC. We next validated our results using the chemical-mediated loss- and gain-of-function approaches. We found that the KLF2 inhibitor, GGPP (geranylgeranyl pyrophosphate) significantly reduces the ND of DPSC. Inversely, KLF2 overexpression was accomplished by using the KLF2 inducer, GGTI-298 (geranylgeranyl transferase inhibitor-298) which accelerated the molecular phenomenon of DPSC’s commitment towards ND, indicating the crucial function of KLF2 in neurogenesis. Moreover, we found that the KLF2 positively regulated autophagy, mitophagy, and the Wnt5a signaling pathway during neurogenesis. Furthermore, we measured the oxygen consumption rate (OCR), and the extracellular acidification rate (ECAR) during ND in the presence of a KLF2 inducer or KLF2 inhibitor using the Xeflux analyzer. We found that most of the ECAR and OCR parameters were significantly increased during ND and inhibition of KLF2 marginally reversed them towards DPSC’s cellular bioenergetics. However, KLF2 overexpression shifted the cellular energy metabolism towards quiescent. Cumulatively, our findings provide the first evidence that the KLF2 critically regulates the neurogenesis of DPSC by inducing autophagy and mitophagy.
In-Situ Quality Assessment and Control in Additive Manufacturing Using Laser Surface Profilometer and Deep Learning Techniques
Authors: Akhavan Javid , Lyu Jiaqi , Manoochehri Souran
Published: October 2022

Additive Manufacturing (AM) has become one of the most popular manufacturing techniques in various fields. Their layer-by-layer printing process allows easier fabrication of complex geometries. However, the quality and accuracy of fabricated artifacts in these techniques have low repeatability. In the era of Industry 4.0 by using emerging sensory and data processing capabilities such as Laser Surface Profilometer (LSP) and Deep Learning (DL), it is possible to improve the repeatability and quality of AM processes. This work presents an in-situ quality assessment and improvement using LSP for data acquisition and DL for data processing and decision making. The utilized LSP module generates a point cloud dataset containing information about the top surface geometry and quality. Once the point cloud data is pre-processed, an improved deep Hybrid Convolutional Auto-Encoder decoder (HCAE) model is used to perform the artifact's quality measurement and statistical representation. The HCAE model's statistical representation is comprised of 9*9 segments, each including four channels with the segment's probability to contain one of four labels, 1) Under-printed region, 2) Normally printed region, 3) Over-printed region, 4) Empty region. This data structure plays a significant role in determining the commands needed to optimize the fabrication process. The implemented HCAE model's accuracy and repeatability were measured by a multi-label multi-output metric developed in this study. The assessments made by HCAE are then used to perform an in-situ process adjustment by manipulating the future layer's fabrication through the G-code modification. By adjusting the machine's print speed and feedrate, the control algorithm exploits the subsequent layer deposition, segment by segment. The algorithm is then tested with two settings that can cause defective parts: severe under-extrusion and over-extrusion conditions. Both test artifacts' quality advanced significantly and converged to an acceptable state by four iterations.
Age- and sex-related trends in body composition among Beijing adults aged 20–60 years: a cross-sectional study
Authors: Zhou Huayi , Gang Xie , laiyuan Luo , He Hui
Published: October 2022

Background:
Obesity is the most serious global epidemic and body composition is the main indicator to evaluate obesity. This study aimed to investigate the changing trends of body composition by age and gender in Beijing adults aged 20–60 years and explore the distribution of obesity rates in different age groups of both sexes under different evaluation criteria.
Methods:
A total of 24,948 adults aged 20–60 years in Beijing, including 10,225 males and 14,192 females, were included, divided into four age groups (20–29, 30–39, 40–49, and ≥50 years) with each decade of age as an age group. Body composition indicators (BMI, fat mass, BF%, muscle mass, visceral fat area, and WHR) were measured in all subjects.
Results:
BMI and total fat mass peaked in males aged 40–49 years (BMI = 25.75 kg/m2, total fat mass = 17.70 kg). Female BMI, fat mass and BF% all increased significantly with age (p < 0.01). Total muscle peaked in males aged 30–39 years and decreased significantly thereafter (p < 0.0001). Visceral fat area and WHR increased significantly with age in both sexes (p < 0.0001). Age was significantly positively correlated with BMI, BF%, fat mass, WHR, and visceral fat area in both sexes (p < 0.0001), and age was negatively correlated with muscle mass in males (standard β = -0.14, p < 0.0001) while positive in female (standard β = 0.05, p < 0.0001). Under the BMI criterion, the obesity rate peaked at 27.33% in males at the age of 20-29 years. Under the BF% criterion, the obesity rate peaked at 17.41% in males at the age of 30-39 years, and increased in females with age. The central obesity rate of both sexes increased with age under the criteria of WHR and visceral fat area.
Conclusion:
The results of this study reveal that age- and sex-related patterns of body composition and obesity change among Beijing adults aged 20–60 years may differ across age groups and that such patterns of change should be considered when developing public health strategies.
Motion stability analysis of cage of rolling bearing under the variable-speed condition
Authors: Tu Wenbing , Liang Jie , Yu Wennian , Shi Zhifeng , Liu Chenlu
Published: October 2022

The fluctuation of cage rotating speed will lead to the strong instability of the cage movement of the rolling bearing, which will seriously affect the operation performance and service life of the bearing. In this paper, a nonlinear dynamic model of cylindrical roller bearing NJ306 is established considering the dynamic contact relationship between the roller, the ring, and the cage. The variation of cage motion characteristics and its internal mechanism under the uniform, acceleration, deceleration, and harmonic speed fluctuation are discussed respectively, and the effects of angular acceleration, angular deceleration, amplitude, and period of fluctuation on cage stability are analyzed. The results show that the motion stability of the cage is poor under the variable-speed condition, and it weakens first and then increases under the acceleration condition. Different from the acceleration condition, the motion stability of the cage decreases gradually with the increase of time during deceleration and increases at the end of deceleration. Moreover, the increase of angular acceleration and deceleration increases the variation range of cage motion stability. Under the condition of harmonic speed fluctuation, the stability of the cage increases and decreases periodically with time. The increase of fluctuation amplitude will enhance the motion stability of the cage under the condition of simple harmonic speed fluctuation, and the fluctuation period is the opposite. The results of this paper can provide a theoretical basis for the design and failure analysis of cage.
The potential novel immune-related prognostic factors for acute myeloid leukemia
Authors: Li Boya , Li Tong , Zheng Xuewei , Zu Xiangyang , Yin Qinan , Qin Ling
Published: October 2022

Background The pathological progression in acute myeloid leukemia (AML) was significantly affected by the immune microenvironment of bone marrow, where the immune-related genes (IRGs) and immune cells are involved in the prognosis of the disease. Studying immune-related components provide new ideas for treatment. Methods The transcriptome data and clinical information of 151 TCGA-LAML and 337 GTEx-whole-blood cohorts were downloaded from the UCSC Xena database. The IRGs were obtained from ImmPort database. Differentially expressed IRGs (DEIGs) were obtained from differentially expressed genes (DEGs). A prognostic model was constructed by COX regression analysis and verified by Kaplan-Meier (K-M) and receiver operating characteristic (ROC) curves in the training and validation cohorts (GEO37642 cohort). The relationship between gene expression level and prognosis was analyzed by K-M method. The independent prognostic factors were screened by univariate and multivariate Cox regression analysis. Relative immune cell composition of AML and healthy samples was calculated using the CIBERSORT algorithm. Results Enrichment analysis revealed that the immune cells and immune-related biology functions participated in AML progression. A prognostic model containing eight genes was constructed. In the training and validation cohorts, the survival rate of the low-risk group was significantly higher than that of the high-risk group. The area under the curve (AUC) values of ROC curves were ≥ 0.7. Among the model, high expression of CANX (P = 0.012), CLEC11A (P = 0.016), and TRH (P = 1.256E-04) was associated with a higher survival rate, while high expression of IL3RA (P = 0.038), KIR2DS4 (P = 0.016), APOBEC3G (P = 1.426E-04), and CKLF (P = 0.015) was associated with lower survival rate. The CANX expression level may affect the sensitivity of some drugs. Age, karyotype, and risk score are independent prognostic factors for AML. The differential level of 17 immune cells was observed between the AML and healthy samples. The levels of Macrophages M1, T cells follicular helper, and T cells CD8 were positively correlated with survival rate. Discussions The prognostic model may be helpful in predicting patient outcomes and 7 IRGs and 3 immune cells may be potential biomarkers and immunotherapy targets for AML in the future.
Philosophers reinforce economists' support for UN climate targets, but disagree on why
Authors: Nesje Frikk , Drupp Moritz , Freeman Mark , Groom Ben
Published: October 2022

The estimated value to society from climate change mitigation is highly sensitive to the long-term social discount rate (SDR). While it is not clear that they possess any special expertise on the ethics of intergenerational welfare, governmental discounting guidance has almost exclusively been influenced by economists. Our study, by contrast, reports the views of a disciplinary group of experts who are most trained in ethical matters; philosophers. We show that, as a group, these experts offer strong support for a real SDR of 2%; a value that is also predominantly backed by economists. We find multidisciplinary support for climate policy paths in line with the UN climate targets when views on discounting determinants are applied within a recent update of the DICE Integrated Assessment Model. However, this apparent agreement hides important differences in views on how the ethics of intergenerational welfare can be better incorporated into climate policy evaluation.
Patients’ and volunteer coaches’ experiences with an informal social network intervention in forensic psychiatric care: a qualitative analysis
Authors: Swinkels L. T. A. , de Koning M. B. , Pol T. M. van der , Dekker J. J. M. , Harmsel J. F. ter , Popma A.
Published: October 2022

Background
Improving supportive social networks in forensic psychiatric patients is deemed important due to the protective effects of such networks on both mental health problems and criminal recidivism. Informal interventions targeted at social network enhancement by volunteers in the community showed positive effects in various patient and offender populations. However, these interventions have not specifically been studied in forensic psychiatric populations. Therefore, forensic psychiatric outpatients’ and volunteer coaches’ experiences with an informal social network intervention were explored in this study.
Methods
This qualitative study was based on semi-structured interviews that were conducted alongside an RCT. Forensic outpatients randomized to the additive informal social network intervention arm of the RCT, and volunteer coaches, were interviewed 12 months after baseline assessment. Interviews were audio-recorded and transcribed verbatim. Reflexive thematic analysis was used to identify and report patterns in the data.
Results
We included 22 patients and 14 coaches in the study. The analysis of interviews revealed five main themes reflecting patients’ and coaches’ experiences: 1) dealing with patient receptivity, 2) developing social bonds, 3) receiving social support, 4) achieving meaningful change, and 5) using a personalized approach. Patient receptivity, including willingness, attitudes, and timing, was a common reported barrier affecting patients’ engagement in the intervention. Both patients’ and coaches’ experiences confirmed that the intervention can be meaningful in developing new social bonds between them, in which patients received social support. Despite, experiences of meaningful and sustainable changes in patients’ social situations were not clearly demonstrated. Coaches’ experiences revealed broadened worldviews and an enhanced sense of fulfillment and purpose. Finally, a personalized, relationship-oriented rather than goal-oriented approach was feasible and preferable.
Conclusions
This qualitative study showed positive experiences of both forensic psychiatric outpatients and volunteer coaches with an informal social network intervention in addition to forensic psychiatric care. Notwithstanding the limitations, the study suggests that additive informal social network interventions provide an opportunity for forensic outpatients to experience new positive social interactions with individuals in the community, which can initiate personal development. Barriers and facilitators to engagement are discussed to improve further development and implementation of the intervention.
Trial registration:
This study is registered at the Netherlands Trial Register (NTR7163, date of registration: 16/04/2018).
A meta-analysis of randomized controlled clinical trials of acupotomy in the treatment of rheumatoid arthritis
Authors: Guo Jingyu , Wang Kefei
Published: October 2022

Objective
To evaluate the efficacy of acupotomy in the treatment of rheumatoid arthritis by meta-analysis.
Methods
Randomized controlled trials of acupotomy in the treatment of rheumatoid arthritis were searched by computer in PubMed, Embase, Cochrane Library, China National Knowledge Infrastructure, Wanfang, VIP and China Biomedical Literature Service System. The retrieval time was up to April 2, 2022. Literature screening and data extraction were performed independently by two researchers. We evaluate the risk of bias in the included studies and stata16 software was used for Meta-analysis.
Results
A total of 13 randomized controlled trials involving 1,103 subjects were included. The clinical efficacy of acupotomy combined therapy for rheumatoid arthritis was improved compared with the control group. Lysholm’s knee function score was improved. The visual analog scale (VAS) was improved. Disease activity score (DAS28) was decreased in 28 joints. Decreased morning stiffness time; Decreased C-reactive protein (CRP), erythrocyte sedimentation rate (ESR) and rheumatoid factor (RF).
Conclusion
The stability of the results of this study is satisfactory, which proves that acupotomy has good efficacy in the treatment of rheumatoid arthritis.
R2R3 MYB Transcription Factor, AhMYB94 Plays a Crucial Role in Stress adaptation of a Salt Susceptible Groundnut Cultivar-K6
Authors: Nulu Jagadeesh Kumar , M Anthony Johnson A , Venkatesh Boya , Jayamma N , Reddy B Manohara , Pandurangaiah M , sudhakar chinta
Published: October 2022

R2R3 MYB transcription factors are involved in plant growth, plant development and abiotic and biotic stress responses. Several MYB genes are involved in salt tolerance in several plant species. Genotypic variation in the expression of a stress responsive regulatory gene,MYB94was observedin groundnut under drought and salinity stress conditions. In this study, a stress-responsive transcription factorAhMYB94was over-expressed in salt-susceptible groundnut cultivar K-6. Relative gene expression analysis revealed thatAhMYB94was significantly higher in groundnut cultivar K9 than in cultivar K6 due to salt treatments. Overexpression ofAhMYB94improved the salt tolerance of groundnut cultivar K6 as evidenced from better growth status, fresh and dry biomass of overexpression lines than wild type (WT) under salt stress conditions. Further, less reactive oxygen species (ROS) accumulated inAhMYB94overexpressing lines when exposed to salt stress, as estimated by diaminobenzidine (DAB) or nitroblue tetrazolium (NBT) histochemical staining. Furthermore, overexpression ofAhMYB94in groundnut confers significantly increased tolerance ability of plants to oxidative damage under salt stress, mainly by increasing the osmolyte content, the activities of antioxidative enzymes such as SOD, and APX, reducing malondialdehyde (MDA) content, and upregulation of the expression of stress-related genes. When exposed to salt stress, theAhMYB94transgenic lines demonstrated a higher Na+and K+content and a lower Na+/K+ratio than the wild type (WT). These results demonstrated thatAhMYB94plays a positive role in enhancing salt tolerance and could be a candidate for improved tolerance of plants to salt stress.
Effect of retained embryos on pregnancy outcomes of in vitro fertilization: a matched retrospective cohort study
Authors: Zhang Huixia , Li Fei , Jin Haixia , Song WenYan , Su Yingchun , Li Gang
Published: October 2022

Objectives
To explore the incidence of retained embryos (REs) in embryo transfer (ET) cycles and its effects on pregnancy outcomes in women undergoing in vitro fertilization (IVF).
Methods
This was a matched retrospective cohort study involving 29,160 ET cycles conducted from March 2016 and February 2021, in which ET cycles without RE were matched to the RE group at a 2:1 ratio. Clinical pregnancy, implantation, miscarriage, and live birth rate were compared between RE and without-RE group.
Results
Our study showed that the overall incidence of REs was 0.33% (95/29,160). The RE rate was significantly associated with the operator who expelled the embryos from the catheter (P < 0.001), suggesting the operator’s technique is a crucial factor affecting the embryo retention. A total of 95 repeat ET cycles due to RE were included in the study group, and 190 ET cycles without RE were matched to the study group (1:2). There were no significant differences between the RE group and matched group in terms of the implantation rate (35.6% vs. 38.0%, P = 0.608), clinical pregnancy rate (47.4% vs. 54.7%, P = 0.240), biochemical pregnancy rate (5.3% vs. 4.7%, P = 0.846), miscarriage rate (11.1% vs. 9.6%, P = 0.781), ectopic pregnancy rate (2.2% vs. 1.9%, P = 1.000), or live birth rate (41.1% vs. 48.9%, P = 0.208).
Conclusions
Our findings demonstrated that immediate retransfer of REs does not significantly affect IVF outcomes, which may provide counselling information for patients when REs are discovered and ET is reattempted. Attention to detail and frequent assessment of operator’s technique may facilitate avoidance of embryo retention.
Prenatal evaluation of chromosomal abnormalities and copy number variations in fetuses with single umbilical artery
Authors: Han Shiyao , Feng Jianwei , Meng Xinyue
Published: October 2022

Background A normal umbilical cord has a single umbilical vein and two umbilical arteries. Single umbilical artery (SUA) is one of the most common umbilical anomaly detected by prenatal ultrasonography. The objective of this study was to evaluate the usefulness of copy number variation sequencing (CNV-seq) and standard karyotyping in fetuses with single umbilical artery (SUA) and to investigate the genetic etiology of prenatal SUA. Methods Data from pregnancies referred for invasive testing and copy number variation sequencing (CNV-seq) due to sonographic diagnosis of fetal with SUA from 2013 to 2022 were obtained retrospectively from the computerized database. The rates of chromosome aberrations and abnormal CNV-seq findings for isolated SUA, SUA accompanied with soft markers and ultrasound malformations were calculated. Results Of the 474 fetuses with SUA that underwent karyotyping, chromosomal abnormalities were detected in fetuses, with a chromosomal abnormality rate of 10.3% (49/474). The use of CNV-seq provides a 10.2% (18/177) incremental yield of detecting pathogenic CNVs in fetuses with SUA and normal karyotype. our study showed that the risk of pathogenic chromosomal abnormalities and copy number variations were increased in the SUA combined malformation or soft markers group compared to that in the isolated SUA group.Meanwhile, fetuses with isolated SUA had an additional 3.4% (6/177) of pathogenic CNVs on top of chromosome aneuploidies. Conclusion CNV-seq could aid in the risk assessment and genetic counseling in fetuses with isolated SUA, Integrating CNV analysis and karyotyping for prenatal diagnosis of SUA in prenatal diagnosis can provide more accurate genetic proof for prenatal counseling and prediction of fetal outcomes.
3D structure of the Campi Flegrei caldera central sector reconstructed through short-period magnetotelluric imaging
Authors: Troiano Antonio , Giuseppe Maria Giulia Di , Isaia Roberto
Published: October 2022

The Campi Flegrei caldera experienced an unrest phase dating to 2005, which primary expression is the impressive ground uplift, accompanied by increasing degassing and seismic activities. Such last two phenomena developed mainly in the caldera central sector, including the Solfatara – Pisciarelli complex. However, the inner structure of such an area is still not defined, and this originates a poor understanding of the ongoing unrest. This paper describes the results of a new magnetotelluric survey performed in the Campi Flegrei caldera central sector. Through the inversion of data collected in 47 independent soundings, a 3D model of the electrical resistivity has been retrieved, which evidenced a partition of the investigated structure. The Agnano – Astroni area seems to be associated with a liquid-dominated geothermal reservoir, whereas the Solfatara – Pisciarelli area seems to be characterized by a single mixed liquid and gasses -dominated geothermal reservoir, which supplies the main caldera fumaroles. The proposed reconstruction of the geometrical characteristics of the hydrothermal system and the primary fluid rising pathways gives substantial clues about the significance of the detected structures in the evolution of the caldera unrest.
A Prototype For Quantum Database In Hybrid Quantum: A Complete Ecosystem
Authors: CHAKRABORTY SAYANTAN
Published: October 2022

This study proposes a prototypeAnd a possibility to converge both this quantum database and classical database. This study mostly identifies the gap between this classical database and quantum database and it proposes a hypothesis and prototype which can be implemented in future products. It is a way that can be used in future industrial product development on hybrid quantum computers. The existing concept used to consider oracle as a black box this study opens up the possibility for the quantum industry to develop the QASAM module so that we can develop a fully quantum database instead of using a classical database as BlackBox.As the Toffoli gate is basically an effective NAND gate it is possible to run any algorithm theoretically in quantum computers. So we will propose a logical design for memory management for the quantum database, security enhancement model, Quantum Recovery Manager & automatic storage management model and more for the quantum database which will ensure the quantum advantages.In this study, we will also explain the Quantum Vector Database as well as the possibility of improvement in duality quantum computing.It opens up a new scope, possibilities and research area in a new approach for quantum database and duality quantum computing.
Busy periods for queues alternating between two modes
Authors: Kleiner Igor , Frostig Esther , Perry David
Published: October 2022

We study the busy period of a single server queueing systemoperating in two alternating modes - working and vacation. In the two modes the systems run as an MX/G/1 queue with disasters, but with different parameters. The vacation mode starts once the number of customers drops to zero. It is terminated randomly (when it is not empty) with a transition to the working mode. At such a transition moment all the customers are transferred to the working mode; the service of the customer being served is lost and it starts from scratch in the working mode. Every busy period starts with a batch arrival into an empty system and terminates at the first time that the number of customers drops to zero. The working and the vacation periods are analyzed too. Finally, we apply the results to obtain the probability generating functions of the number of customers in the working, as well as in the vacation periods.
A DFT/TDDFT investigation on chromone derivatives: fluorescence and electronic properties
Authors: Huang Pei , Lu Jiufu , Jin Lingxia , Liu Ernu , Li Li
Published: October 2022

The development of quick and precise detection technologies for active compounds in vivo is critical for disease prevention, diagnosis, and pathological investigation. The fluorescence signal of the fluorophore usually defines the probe's sensitivity to the chemical being examined. Many natural compounds containing flavone and isoflavone scaffolds exhibit a certain amount fluorescence, albeit with poor fluorescence quantum yields. Therefore, we used density functional theory (DFT) and time-dependent density functional theory (TDDFT) calculations to investigate the fluorescence characteristics of chromium-derived fluorophores in more depth. Different substituents are introduced at different positions of the chromone. As weak electron donor groups, alkyl and aromatic groups were discovered to have varying quantum yields on the fluorophore scaffold, and longer alkyl chains are favorable to enhance fluorescence quantum yield. In comparison to the amino group, substituted amino group can avoid group rotation, and the introduction of cyclic amines such as pyrrolidine and heterocyclic amines can improve optical characteristics. The electron-donating methoxy group at position 6 helps to increase the fluorescence quantum yield.
Clinical significance of reactive thrombocytosis in the course of acute pancreatitis
Authors: Chiba Nobutaka , Sugita Atsunori , Mizuochi Minori , Sato Jun , Saito Takeshi , Sakurai Atsushi , Kinoshita Kosaku
Published: October 2022

Background
Reactive thrombocytosis occurs secondary to systemic infections, inflammatory, and other conditions. The relationship between thrombocytosis and acute pancreatitis (AP) in inflammatory diseases is uncertain. This study aimed to evaluate the clinical significance of thrombocytosis in AP patients during hospitalization.
Methods
Subjects within 48 hours of AP onset were consecutively enrolled over 6 years. Platelet counts of ≥ 450,000/µL were defined as thrombocytosis, < 100,000/µL as thrombocytopenia, and other counts as normal. We compared clinical characteristics, including the rate of Severe AP (SAP) assessed by the Japanese Severity Score; blood markers, including hematologic and inflammatory factors and pancreatic enzymes during hospitalization; and pancreatic complications and outcomes in the three groups.
Results
A total of 108 patients were enrolled. Although, SAP was more common in patients with thrombocytosis and thrombocytopenia (87.9% and 100%, respectively), the differences in lymphocytes and C-reactive protein, lactase dehydrogenase, or antithrombin levels, which are factors of the systemic inflammatory response, and mean platelet volume, an indicator of platelet activation were observed among patients with thrombocytosis and thrombocytopenia during hospitalization. Regarding pancreatic complications and outcomes, patients with thrombocytosis and thrombocytopenia had higher acute necrotic collection (ANC), pancreatic necrosis, intestinal paralysis, respiratory dysfunction, and pancreatic-related infection levels than those in patients with normal platelet levels. The relationship between pancreatic complications and thrombocytosis was assessed by multivariate logistic regression; the odds ratios for the development of ANC, pancreatic necrosis and pancreatic-related infections were 7.360, 3.735 and 9.815, respectively.
Conclusions
Thrombocytosis during hospitalization for AP suggested the development of local pancreatic complications and pancreatic-related infections.
TRIM17-mediated ubiquitination and degradation of RBM38 promotes cisplatin resistance in non-small cell lung cancer
Authors: Zhong Tian , Zhang Jing , Liu Xingren , Li Hongmin
Published: October 2022

Cisplatin (CDDP)-based chemotherapy is commonly used to treat advanced non-small cell lung cancer (NSCLC). However, the efficacy is limited by the development of drug resistance. Tripartite motif (TRIM) proteins typically have E3 ubiquitin ligase activities and modulate protein stability. In the present study, we screened for chemosensitivity-regulating TRIM proteins using CDDP-resistant NSCLC cell lines. We show that TRIM17 is upregulated in CDDP-resistant NSCLC cells and tumors compared to CDDP-sensitive counterparts. NSCLC patients with high TRIM17 expression in tumors have shorter progression-free survival than those with low TRIM17 expression after CDDP chemotherapy. Knockdown of TRIM17 increases the sensitivity of NSCLC cells to CDDP both in vitro and in vivo. In contrast, overexpression of TRIM17 promotes CDDP resistance in NSCLC cells. TRIM17-mediated CDDP resistance is associated with attenuation of reactive oxygen species (ROS) production and DNA damage. Mechanistically, TRIM17 interacts with RBM38 and promotes K48-linked ubiquitination and degradation of RBM38. TRIM17-induced CDDP resistance is remarkably reversed by RBM38. Additionally, RBM38 enhances CDDP-induced production of ROS. In conclusion, TRIM17 upregulation drives CDDP resistance in NSCLC largely by promoting RBM38 ubiquitination and degradation. Targeting TRIM17 may represent a promising strategy for improving CDDP-based chemotherapy in NSCLC.
Effects of temperature and light on the stability of the blast effector complex APikL2A/sHMA25 as determined by molecular dynamic analyses
Authors: Zhao Ling , Zhang Ting , Luo Yanjie , Li Lin , Cheng Ruhong , Shi Zhigang , Wang Genping , Ren Tiancong
Published: October 2022

Magnaporthe oryzae is the causal agent of rice blast. Understanding how abiotic stress affects the resistance of plants to this disease is useful for designing disease control strategies. Here, the effects of temperature and light (supplied under an oscillating electric field) on the effector complex comprising APikL2A from M. oryzae and sHMA25 from foxtail millet were investigated by molecular dynamic (MD) simulations using the GROMACS package. While the structure of APikL2A/sHMA25 remained relatively stable in a temperature range from 290 K (16.85°C) to 320 K (46.85°C), the dome shape of the temperature-dependent binding free energy curve indicated that there was minimum binding affinity between APikL2A and sHMA25 at 300 K – 310 K. This coincided with the optimum infectivity temperature, suggesting that decoupling of the two polypeptides may play a role in the infection process. A strong oscillating electric field destroyed the structure of APikL2A/sHMA25, although it was stable under weaker electric fields. An increase in binding free energy was discovered in these electric field-dependent simulations.
The intensity of eco-environmental constraints and green innovation efficiency
Authors: Wu Mingran
Published: October 2022

In this study, a spatial econometric model was constructed by combining the intensity of eco-environmental constraints, the efficiency of green innovation and a series of high-quality economic development indicators. The impact of environmental constraint intensity on green innovation efficiency was empirically analysed on Chinese provincial panel data, and the impact was decomposed into direct and indirect (spillover). The results showed that (1) from 2011 to 2020, the overall performance of China's green innovation efficiency was relatively fine, and the efficiency value has been greatly improved in the later stage, especially in 2020. In addition, regional green innovation performance was positively correlated with economic development. (2) The strengthening of eco-environmental constraints was not conducive to the improvement of regional green innovation efficiency, but the scale and agglomeration effect of industrial enterprises and the increase in foreign trade activities were conducive to efficiency improvement. (3) The direct effect of eco-environmental constraints and firm size on green innovation efficiency in this region was positive, while the indirect effect and total effect were negative. The direct effect of investment openness and industrial agglomeration degree was negative, while the indirect effect and total effect were positive. All effects of industrial structure were 0. Therefore, the government needs to set up reasonable methods of environmental regulation, complete and optimize the industrial chain, and formulate reasonable regional development plans.
Application of Surface-To-Roadway TEM for detecting deep aquifer in Coal Field, an example from North China
Authors: Chen Weiying , Xue Guoqiang , Yu Jincun , Lei Kangxin , Song Wanting
Published: October 2022

Transient electromagnetic methods (TEM) are effective tools for the hydrogeological investigations of coal mines. However, the traditional ground-based and underground-based TEMs typically fail to detect the deeply buried water-bearing bodies in the majority of coal mines in northern China, where the quaternary overburden is generally thick and many aquifers exist. In the present study, a surface-to-roadway TEM was proposed which utilized grounded-wire sources to transmit signals with large moments on the surface, and required receivers which had the ability to record signals with high resolution in underground mining roadways. First of all, the proposed device was introduced. Subsequently, a synthetic model was established based on the existing information of Zhuxianzhuang coal field in Anhui Province, China, along with the analysis of forward and inversion results. Finally, the acquisition and processing of field data were conducted. Both the synthetic and field results demonstrated the feasibility and superiority of the proposed method. The deeply buried aquifers were able to be better characterized by the surface-to-roadway configuration than the ground-based surveys.
Identification and genetic characterization of a new totivirus from Bursera graveolens in western Ecuador
Authors: Cornejo Juan Francisco , Alvarez Robert , Mollov Dimitre , Quito-Avila Diego
Published: October 2022

The complete genomic sequence of a previously uncharacterized virus provisionally named Bursera graveolens totivirus 1 (BgTV-1) was obtained from Bursera graveolens (Kunth) Triana & Planch., a tree known as “palo santo” in Ecuador. The BgTV-1 genome is monopartite double-stranded RNA (dsRNA) and 4,794 nucleotides (nt) long (GenBank accession number ON988291). Phylogenetic analysis of the capsid protein (CP) and RNA-dependent-RNA-polymerase (RdRp) placed BgTV-1 in a clade with other plant-associated totiviruses. Amino acid (aa) sequence comparisons of BgTV-1 putative proteins showed the highest identities to those of taro-associated totivirus L (QFS21890.1-QFS21891.1) and Panax notoginseng virus A (YP_009225664.1- YP_009225665.1) with 51.4% and 49.8%, for the CP, and 56.4% and 55.2% for the RdRp, respectively. BgTV-1 was not detected in any of the two endophytic fungi grown in BgTV-1-positive B. graveolens leaves, suggesting a plant totivirus. Based on distinct host and low aa identities between the CP of BgTV-1 and counterparts from closest relatives, the virus described in this study should be assigned as a new member of the genus Totivirus.
Astrocytic Cebpd Regulates Pentraxin 3 Expression to Promote Fibrotic Scar Formation after Spinal Cord Injury
Authors: Wang Shao-Ming , Hsu Jung-Yu C , Ko Chiung-Yuan , Wu Hsiang-En , Hsiao Yu-Wei , Wang Ju-Ming
Published: October 2022

Astroglial-fibrotic scars resulted from spinal cord injury affect motor and sensory function, leading to paralysis. In particular, the fibrotic scar is a main barrier that disrupts neuronal regeneration after spinal cord injury. However, the association between astrocytes and fibrotic scar formation is not yet understood. We have previously demonstrated that the transcriptional factor Cebpd contributes to astrogliosis, which promotes glial scar formation after spinal cord injury. Herein, we show that fibrotic scar formation was decreased in the epicentre region in Cebpd−/− mice after contusive spinal cord injury and astrocytic Cebpd promoted fibroblast migration through secretion of Ptx3. Furthermore, the expression of Mmp3 was increased under recombinant protein Ptx3 treatment in fibroblasts by observing microarray data, resulting in fibroblast migration. In addition, regulation of Mmp3 occurs through the NFkB signaling pathway by using an irreversible inhibitor of IκBα phosphorylation in pretreated fibroblasts. Of note, we used the synthetic peptide RI37, which blocks fibroblast migration and decreases fibroblast Mmp3 expression in IL-1b-treated astrocyte conditioned media. Collectively, our data suggest that fibroblast migration can be affected by astrocytic Cebpd through the Ptx3/NFkB/Mmp3 axis pathway and that the RI37 peptide may act as a therapeutic medicine to inhibit fibrotic scar formation after spinal cord injury.
Periodic solutions of a class of indefinite singular differential equations
Authors: Liang Zaitao , Zhang Xiuqiang
Published: October 2022

In this paper, we establish some sufficient conditions for the existence of positive periodic solutions of a more general indefinite singular differential equation. Our results are applicable to the case of a strong singularity as well as the case of a weak singularity. Recent results in the literature are generalized and improved.
2020 MSC: 34C25
Establishment of CRISPR-Cas9 system in Bifidobacteria animalis AR668
Authors: Li Jiao , Song Xin , Xiong Zhiqiang , Wang Guangqiang , Xia Yongjun , Yang Yijin , Ai Lianzhong
Published: October 2022

Bifidobacteria are representative intestinal probiotics that have extremely high application value in the food and medical fields. However, the lack of molecular biology tools limits the research on functional genes and mechanisms of bifidobacteria. The application of accurate and efficient CRISPR system to genome engineering can fill the gap in efficient genetic tools for bifidobacteria. In this study, CRISPR system of B. animalis AR668 was established, which successfully knocked out gene 0348and gene 0208. The influence of different homology arms and fragments on the knockout effect of the system was explored. In addition, the inducible plasmid curing system of bifidobacteria was innovatively established. This study contributes to the genetic modification and functional mechanism analysis of bifidobacteria.
Personalized Federated Learning with Model Interpolation among Client Clusters and its Application in Smart Home
Authors: Yang Zhikai , Liu Yaping , Zhang Shuo , Zhou Keshen
Published: October 2022

The proliferation of high-performance personal devices and the widespread deployment of machine learning (ML) applications have led to two consequences: the volume of private data from individuals or groups has exploded over the past few years; and the traditional central servers for training ML models have experienced communication and performance bottlenecks in the face of massive amounts of data. However, this reality also provides the possibility of keeping data local for ML training and fusing models on a broader scale. As a new branch of ML application, Federated Learning (FL) aims to solve the problem of multi-party joint learning on the premise of protecting personal data privacy. However, due to the heterogeneity of devices, including network connection, network bandwidth, computing resources, etc., it is unrealistic to train, update and aggregate models in all devices in parallel, while personal data is often not independent and identically distributed (Non-IID) due to multiple reasons. This reality poses a challenge to the speed and convergence of FL. In this paper, we propose the pFedCAM algorithm, which aims to improve the robustness of the FL system to device heterogeneity and Non-IID data, while achieving some degree of federation model personalization. pFedCAM is based on the idea of clustering and model interpolation by classifying heterogeneous clients and performing FedAvg algorithm in parallel, and then combining them into personalized federated global models by inter-cluster model interpolation. Experiments show that the accuracy of pFedCAM improves 10.3% on Fashion-MNIST and 11.3% on CIFAR-10 compared to the benchmark in the case of Non-IID data. In the end, we applied pFedCAM to HomeProtect, a smart home privacy protection framework we designed, and achieved good practical results in the case of flame recognition.
Fault-tolerant quantum anonymous communication protocols against collective noise
Authors: Liang Xiang-qian , Liu Bing-xin , Xu Guang-bao , Jiang Dong-huan , Liang An-hui
Published: October 2022

Considering the influence of collective-dephasing (C-D) noise and collective-rotation (C-R) noise, by utilizing controlled-NOT operation, controlled-Z operation and other unitary operations, we proposed the fault-tolerant quantum protocols to realize the quantum anonymous communication against C-D noise and C-R noise, respectively. Correctness analysis shows that the encrypted quantum states can not be affected by the collective noise. Security analysis shows that the protocols can ensure the anonymity of the sender and receiver and privacy of quantum information.