bluuebunny/crossref_metadata_2025 huggingface dataset:
Oclacitinib and Myxoma Virus Therapy in Dogs with High-Grade Soft Tissue Sarcoma
Authors: Ashton Laura V. , Weishaar Kristen M. , Séguin Bernard , MacNeill Amy L.
Published: August 2023

Human rhabdomyosarcomas are rarely cured by surgical resection alone. This is also true for high-grade soft tissue sarcomas in dogs. Dogs with spontaneous sarcoma are good models for clinical responses to new cancer therapies. Strategic combinations of immunotherapy and oncolytic virotherapy (OV) could improve treatment responses in canine and human cancer patients. To develop an appropriate combination of immunotherapy and OV for dogs with soft tissue sarcoma (STS), canine cancer cells were inoculated with myxoma viruses (MYXVs) and gene transcripts were quantified. Next, the cytokine concentrations in the canine cancer cells were altered to evaluate their effect on MYXV replication. These studies indicated that, as in murine and human cells, type I interferons (IFN) play an important role in limiting MYXV replication in canine cancer cells. To reduce type I IFN production during OV, oclacitinib (a JAK1 inhibitor) was administered twice daily to dogs for 14 days starting ~7 days prior to surgery. STS tumors were excised, and MYXV deleted for serp2 (MYXV∆SERP2) was administered at the surgical site at two time points post-operatively to treat any remaining microscopic tumor cells. Tumor regrowth in dogs treated with OV was decreased relative to historical controls. However, regrowth was not further inhibited in patients given combination therapy.
Intracellular cAMP Signaling Pathway via Gs Protein-Coupled Receptor Activation in Rat Primary Cultured Trigeminal Ganglion Cells
Authors: Kunioku Yuki , Kimura Maki , Ouchi Takehito , Fukuda Kenichi , Shibukawa Yoshiyuki
Published: August 2023

G protein-coupled receptors in trigeminal ganglion (TG) neurons are often associated with sensory mechanisms, including nociception. We have previously reported the expression of P2Y12 receptors, which are Gi protein-coupled receptors, in TG cells. Activating P2Y12 receptors decreased the intracellular free Ca2+ concentration ([Ca2+]i). This indicated that intracellular adenosine 3′,5′-cyclic monophosphate (cAMP) levels can mediate Ca2+ signaling in TG cells. Here, we report more extensive-expression patterns of Gs protein-coupled receptors in primary cultured TG neurons isolated from 7-day-old newborn Wistar rats and further examine the roles of these receptors in cAMP signaling using the BacMam sensor in these neurons. To identify TG neurons, we also measured [Ca2+]i using fura-2 in TG cells and measured intracellular cAMP levels. TG neurons were positive for Gαs protein-coupled receptors, beta-2 adrenergic (β2), calcitonin gene-related peptide (CGRP), adenosine A2A (A2A), dopamine 1 (D1), prostaglandin I2 (IP), and 5-hydroxytriptamine 4 (5-HT4) receptor. Application of forskolin (FSK), an activator of adenylyl cyclase, transiently increased intracellular cAMP levels in TG neurons. The application of a phosphodiesterase inhibitor augmented the FSK-elicited intracellular cAMP level increase. These increases were significantly suppressed by the application of SQ22536, an adenylyl cyclase inhibitor, in TG neurons. Application of agonists for β2, CGRP, A2A, D1-like, IP, and 5-HT4 receptors increased intracellular cAMP levels. These increases were SQ22536-sensitive. These results suggested that TG neurons express β2, CGRP, A2A, D1, IP, and 5-HT4 receptors, and the activations of these Gαs protein-coupled receptors increase intracellular cAMP levels by activating adenylyl cyclase.
Development and Validation of a High-Performance Liquid Chromatography with Tandem Mass Spectrometry (HPLC-MS/MS) Method for Quantification of Major Molnupiravir Metabolite (β-D-N4-hydroxycytidine) in Human Plasma
Authors: Komarov Timofey , Karnakova Polina , Archakova Olga , Shchelgacheva Dana , Bagaeva Natalia , Popova Mariia , Karpova Polina , Zaslavskaya Kira , Bely Petr , Shohin Igor
Published: August 2023

Molnupiravir is an antiviral drug against viral RNA polymerase activity approved by the FDA for the treatment of COVID-19, which is metabolized to β-D-N4-hydroxycytidine (NHC) in human blood plasma. A novel method was developed and validated for quantifying NHC in human plasma within the analytical range of 10–10,000 ng/mL using high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) to support pharmacokinetics studies. For sample preparation, the method of protein precipitation by acetonitrile was used, with promethazine as an internal standard. Chromatographic separation was carried out on a Shim-pack GWS C18 (150 mm × 4.6 mm, 5 μm) column in a gradient elution mode. A 0.1% formic acid solution in water with 0.08% ammonia solution (eluent A, v/v) and 0.1% formic acid solution in methanol with 0.08% ammonia solution mixed with acetonitrile in a 4:1 ratio (eluent B, v/v) were used as a mobile phase. Electrospray ionization (ESI) was used as an ionization source. The developed method was validated in accordance with the Eurasian Economic Union (EAEU) rules, based on the European Medicines Agency (EMA) and Food and Drug Administration (FDA) guidelines for the following parameters and used within the analytical part of the clinical study of molnupiravir drugs: selectivity, suitability of standard sample, matrix effect, calibration curve, accuracy, precision, recovery, lower limit of quantification (LLOQ), carryover, and stability.
Cardiovascular Consequences of Acute Kidney Injury: Treatment Options
Authors: Voicehovska Julija G. , Trumpika Dace , Voicehovskis Vladimirs V. , Bormane Eva , Bušmane Inara , Grigane Anda , Moreino Eva , Lejnieks Aivars
Published: August 2023

Soon after haemodialysis was introduced into clinical practice, a high risk of cardiac death was noted in end-stage renal disease. However, only in the last decade has it become clear that any renal injury, acute or chronic, is associated with high overall and cardiovascular lethality. The need for early recognition of kidney damage in cardiovascular pathology to assess risk and develop tactics for patient management contributed to the emergence of the concept of the “cardiorenal syndrome” (CRS). CRS is a pathophysiological disorder of the heart and kidneys in which acute or chronic dysfunction of one of these organs leads to acute or chronic dysfunction of the other. The beneficial effect of ultrafiltration as a component of renal replacement therapy (RRT) is due to the elimination of hyperhydration, which ultimately affects the improvement in cardiac contractile function. This review considers the theoretical background, current status of CRS, and future potential of RRT, focusing on the benefits of ultrafiltration as a therapeutic option.
P300 Event-Related Potential Predicts Cognitive Dysfunction in Patients with Vestibular Disorders
Authors: Ma Xiaobao , Shen Jiali , Sun Jin , Wang Lu , Wang Wei , He Kuan , Chen Xiangping , Zhang Qin , Jin Yulian , Gao Dekun , Duan Maoli , Yang Jun , Chen Jianyong , He Jingchun
Published: August 2023

Objective: Our aim was to determine the correlation between cognitive impairment and P300 event-related potential (ERP) in older adults with vertigo and imbalance, which further provides a reference for clinical diagnosis and patients’ rehabilitation. Methods: A total of 79 older adult patients with vertigo and imbalance in our outpatient department from January 2022 to December 2022 were selected and divided into the mild group (n = 20), moderate group (n = 39), and severe group (n = 20) according to the Dizziness Handicap Inventory (DHI). The auditory P300 component of event-related potentials (ERPs), Generalized Anxiety Disorder Questionnaire-7 (GAD-7), Patient Health Questionnaire-9 (PHQ-9), and Mini-Mental State Examination (MMSE) were used to evaluate depression, anxiety, and cognitive function in these patients, respectively. Results: The P300 latencies of the different severity groups were 292 ± 10 ms, 301 ± 8 ms, and 328 ± 5 ms, respectively, and the differences were statistically significant (p = 0.010). The P300 amplitudes of the different severity groups were 14.4 ± 2.6 μV, 3.9 ± 0.8 μV, and 5.1 ± 1.4 μV, respectively, and the differences were also statistically significant (p = 0.004). There was no statistically significant difference in the DHI evaluation or VAS visual simulation scoring between the two groups (p = 0.625, and 0.878, respectively). Compared with the short-course group, the long-course group showed prolonged P300 latency and decreased amplitude, higher scores in PHQ-9 and GAD-7, and lower scores in MMSE, and all the differences were statistically significant (p = 0.013, 0.021, 0.006, 0.004, and 0.018, respectively). Conclusion: Older patients with more severe symptoms of vertigo and imbalance are at higher risk of developing abnormal cognitive function. The P300 can be used as an objective neurophysiological test for the assessment of cognitive function relevant to elderly patients with vertigo and imbalance.
The Effects of Non-Pharmacological Interventions in Fibromyalgia: A Systematic Review and Metanalysis of Predominants Outcomes
Authors: Hong-Baik Isabel , Úbeda-D’Ocasar Edurne , Cimadevilla-Fernández-Pola Eduardo , Jiménez-Díaz-Benito Victor , Hervás-Pérez Juan Pablo
Published: August 2023

(1) Fibromyalgia (FM) is a chronic musculoskeletal condition with multiple symptoms primarily affecting women. An imbalance in cytokine levels has been observed, suggesting a chronic low-grade inflammation. The main aim of the meta-analysis was to examine the effect of multimodal rehabilitation on cytokine levels and other predominant variables in patients with FM. Furthermore, to examine which non-pharmacological tools have been used to investigate the effects that these can have on cytokines in FM patients. (2) Methods: Searches were conducted in PubMed, Scopus, Web of Science, Cochrane, and ScienceDirect databases. This systematic review and metanalysis followed the PRISMA statement protocol. The methodological quality of the studies was assessed using the PEDro scale, the risk of bias followed the Cochrane Manual 5.0.1, and the GRADE system was used for rating the certainty of evidence. (3) Results: Of 318 studies found, eight were finally selected, with a sample size of 320 women with a mean age of 57 ± 20. The proinflammatory cytokines IL-1β, IL-6, IL-8 and TNF-α were the most studied. Resistance exercise, aquatic exercise, dynamic contractions, cycling, treadmill, and infrared therapy were the main non-pharmacological tools used. (4) Conclusions: The systematic review with meta-analysis found evidence of elevated cytokine levels in patients with FM, suggesting low chronic inflammation and a possible contribution to central sensitization and chronic pain. However, the effects of physiotherapeutic interventions on cytokine levels are variable, highlighting the importance of considering different factors and the need for further research.
Automatic Detection and Classification of Epileptic Seizures from EEG Data: Finding Optimal Acquisition Settings and Testing Interpretable Machine Learning Approach
Authors: Statsenko Yauhen , Babushkin Vladimir , Talako Tatsiana , Kurbatova Tetiana , Smetanina Darya , Simiyu Gillian Lylian , Habuza Tetiana , Ismail Fatima , Almansoori Taleb M. , Gorkom Klaus N.-V. , Szólics Miklós , Hassan Ali , Ljubisavljevic Milos
Published: August 2023

Deep learning (DL) is emerging as a successful technique for automatic detection and differentiation of spontaneous seizures that may otherwise be missed or misclassified. Herein, we propose a system architecture based on top-performing DL models for binary and multigroup classifications with the non-overlapping window technique, which we tested on the TUSZ dataset. The system accurately detects seizure episodes (87.7% Sn, 91.16% Sp) and carefully distinguishes eight seizure types (95–100% Acc). An increase in EEG sampling rate from 50 to 250 Hz boosted model performance: the precision of seizure detection rose by 5%, and seizure differentiation by 7%. A low sampling rate is a reasonable solution for training reliable models with EEG data. Decreasing the number of EEG electrodes from 21 to 8 did not affect seizure detection but worsened seizure differentiation significantly: 98.24 ± 0.17 vs. 85.14 ± 3.14% recall. In detecting epileptic episodes, all electrodes provided equally informative input, but in seizure differentiation, their informative value varied. We improved model explainability with interpretable ML. Activation maximization highlighted the presence of EEG patterns specific to eight seizure types. Cortical projection of epileptic sources depicted differences between generalized and focal seizures. Interpretable ML techniques confirmed that our system recognizes biologically meaningful features as indicators of epileptic activity in EEG.
Different Methods for Assessing Tooth Colour—In Vitro Study
Authors: Dias Susana , Dias Joana , Pereira Ruben , Silveira João , Mata António , Marques Duarte
Published: August 2023

Colour assessment using digital methods can yield varying results, and it is important for clinicians to recognize the potential variability intra and inter-device. This study aimed to compare the L*a*b* values of VITA Classical (VC) and VITA Toothguide 3D-MASTER (VM) guides using two methods, SpectroShade (SS) and eLAB. Thirty-four measurements per tab were performed by a single operator across three batches of each guide. Intraclass correlation coefficients (ICC) between batches were calculated. Values <0.5, 0.5–0.75, 0.75–0.9, and >0.90 were classified as poor, moderate, good, and excellent reliability, respectively. Results were reported as mean and standard deviation of the L*a*b* values and respective colour differences (ΔE00) for each tab and method. Statistical analyses were performed with an independent t-test, α = 0.05. ICC values between batches were excellent for all L*a*b*, except for a* component in eLAB. There were statistically significant differences between methods in most L*a*b* values. The intra-device mean ΔE00 was 0.5 ± 0.6 for VC, 0.5 ± 0.8 for VM in SS, 1.1 ± 0.8 for VC, 1.1 ± 0.9 for VM in eLAB. The mean ΔE00 inter-device was 4.9 ± 1.7 for VC, 5.0 ± 1.7 for VM. Both methods demonstrated good internal consistency, with high ICC values and low intra-device colour differences, but exhibited high variability between methods, higher for a* the component.
Molecularly Targeted Fluorescent Sensors for Visualizing and Tracking Cellular Senescence
Authors: He Zhirong , Xu Kun , Li Yongming , Gao Han , Miao Tingting , Zhao Rui , Huang Yanyan
Published: August 2023

Specific identification and monitoring of senescent cells are essential for the in-depth understanding and regulation of senescence-related life processes and diseases. Fluorescent sensors providing real-time and in situ information with spatiotemporal resolution are unparalleled tools and have contributed greatly to this field. This review focuses on the recent progress in fluorescent sensors for molecularly targeted imaging and real-time tracking of cellular senescence. The molecular design, sensing mechanisms, and biological activities of the sensors are discussed. The sensors are categorized by the types of markers and targeting ligands. Accordingly, their molecular recognition and fluorescent performance towards senescence biomarkers are summarized. Finally, the perspective and challenges in this field are discussed, which are expected to assist future design of next-generation sensors for monitoring cellular senescence.
Early Measures of TBI Severity Poorly Predict Later Individual Impairment in a Rat Fluid Percussion Model
Authors: Hetzer Shelby M. , Casagrande Andrew , Qu’d Dima , Dobrozsi Nicholas , Bohnert Judy , Biguma Victor , Evanson Nathan K. , McGuire Jennifer L.
Published: August 2023

Background: Multiple measures of injury severity are suggested as common data elements in preclinical traumatic brain injury (TBI) research. The robustness of these measures in characterizing injury severity is unclear. In particular, it is not known how reliably they predict individual outcomes after experimental TBI. Methods: We assessed several commonly used measures of initial injury severity for their ability to predict chronic cognitive outcomes in a rat lateral fluid percussion (LFPI) model of TBI. At the time of injury, we assessed reflex righting time, neurologic severity scores, and 24 h weight loss. Sixty days after LFPI, we evaluated working memory using a spontaneous alternation T-maze task. Results: We found that righting time and weight loss had no correlation to chronic T-maze performance, while neurologic severity score correlated weakly. Discussion: Taken together, our results indicate that commonly used early measures of injury severity do not robustly predict longer-term outcomes. This finding parallels the uncertainty in predicting individual outcomes in TBI clinical populations.
Age of Bilingual Onset Shapes the Dynamics of Functional Connectivity and Laterality in the Resting-State
Authors: Sheng Yucen , Yang Songyu , Rao Juan , Zhang Qin , Li Jialong , Wang Dianjian , Zheng Weihao
Published: August 2023

Bilingualism is known to enhance cognitive function and flexibility of the brain. However, it is not clear how bilingual experience affects the time-varying functional network and whether these changes depend on the age of bilingual onset. This study intended to investigate the bilingual-related dynamic functional connectivity (dFC) based on the resting-state functional magnetic resonance images, including 23 early bilinguals (EBs), 30 late bilinguals (LBs), and 31 English monolinguals. The analysis identified two dFC states, and LBs showed more transitions between these states than monolinguals. Moreover, more frequent left–right switches were found in functional laterality in prefrontal, lateral temporal, lateral occipital, and inferior parietal cortices in EBs compared with LB and monolingual cohorts, and the laterality changes in the anterior superior temporal cortex were negatively correlated with L2 proficiency. These findings highlight how the age of L2 acquisition affects cortico-cortical dFC pattern and provide insight into the neural mechanisms of bilingualism.
Do Illegitimate Tasks Lead to Work Withdrawal Behavior among Generation Z Employees in China? The Role of Perceived Insider Status and Overqualification
Authors: Fan Pengxiang , Zhang Hao , Yang Songlin , Yu Zixuan , Guo Ming
Published: August 2023

Generation Z employees in the workplace cause a management challenge that enterprises have recently faced. The unique characteristics of Generation Z employees necessitate an urgent update to the knowledge of organizational management. However, few studies of the literature focus on the workplace behaviors of Generation Z. This study proposes that illegitimate tasks may lead to work withdrawal behavior among Generation Z employees. Based on the equity theory model, this study constructed a moderated mediation model to explore the impact of illegitimate tasks on the work withdrawal behavior of Generation Z employees, as well as the mediating role of perceived insider status and the moderating role of perceived overqualification. The analysis of survey data from 283 Generation Z employees in China at two time points found that illegitimate tasks are positively correlated with work withdrawal behavior. At the same time, the mediating role of perceived insider status was successfully confirmed. The results also showed that perceived overqualification strengthened the effect of illegitimate tasks on work withdrawal behavior and the mediating effect of perceived insider status. This study offers new insights into the management and development of Generation Z employees and the sustainable evolution of workplace relationships from both theoretical and practical perspectives.
The Geo-Distribution and Spatial Characteristics of Tulou Dwellings in Chaozhou, Guangdong, China
Authors: Chen Dan , Su Jiaying , Ye Jianping
Published: August 2023

Tulou dwellings in Southeast China have captivated global interest due to their distinctive appearance, sustainable construction materials and technologies, and their defensive and collective housing functions. Despite several being recognized as World Cultural Heritage sites, the vast majority of tulou buildings are undergoing irreversible decline and destruction, necessitating a comprehensive and systematic study. Taking 83 tulou buildings in Raoping County, Chaozhou City, Guangdong Province as the research object, this study reconstructs the historical scenes and systematically reveals the emergence, popularity, and consolidation process of tulou dwellings as integrated defensive and residential buildings for ordinary people by conducting a comprehensive analysis of historical documents and local chronicles. Based on an extensive field investigation, the study systematically analyzes the geospatial distribution and the spatial characteristics of Raoping tulou and its residential unit. The results demonstrate the adaptability and flexibility of tulou dwellings, showcasing their developmental process and revealing the inclusive nature of these traditional residences, as well as the initiative of those who reside within them. The research findings contribute to a more dynamic, comprehensive, and authentic understanding of tulou and Chinese traditional residences, providing valuable references for the preservation and sustainable development of tulou architectural heritage.
Exposure of Elderly People to Indoor Air Pollutants in Wanxia Nursing Home
Authors: Zong Hua , Tian Lei , Cao Zhimeng , Luo Minjie
Published: August 2023

The elderly residing in nursing homes are typically more advanced in age, have more health issues, and spend more time indoors than other elderly people. This study explored the indoor air quality in the Wanxia nursing home, the largest private nursing home in Chengdu, China, based on long-term measurement data. Air pollutant sensors measured the level of air pollution in the Wanxia nursing home from March 2021 to February 2022. This study obtained several important results: (1) The indoor air quality index (IAQI) of the Wanxia nursing home was at a low pollution level in spring, summer, and autumn, and at a moderate pollution level in winter. PM concentration played the most important role in determining indoor air quality; (2) During winter, the 24 h mean indoor concentrations of PM2.5 and PM10 were close to or even exceeded the standard limits. During winter and summer nights, indoor CO2 levels were very close to or greater than 1000 ppm. During spring and summer nights, the indoor TVOC concentrations exceeded the limit (0.45 mg/m3); (3) Apart from HCHO concentrations in autumn, the levels of other indoor air pollutants were significantly influenced by their outdoor levels. In addition, the seasonal indoor/outdoor (I/O) ratios of CO2 and TVOCs exceeded 1; and (4) Indoor CO2 levels were closely related to indoor temperature (Ta) and relative humidity (RH) in each season. PM10 concentration correlated with Ta and RH in summer, while PM2.5 concentration did not correlate with Ta and RH in winter. The indoor TVOC level positively correlated with RH. Lastly, the indoor HCHO level was minimally influenced by changes in Ta and RH. Due to the above results, this study proposes targeted strategies for improving indoor air quality in nursing homes.
Source Identification and Characterization of Indoor Particulate Matter in Potala Palace Museum
Authors: Zhang Ge , Li Wenqing , Cheng Qian , Zhou Zhipeng , Wang Qiaochu , Peng Zhiyuan
Published: August 2023

This study aims to determine the sources of indoor particulate matter at the Potala Palace Museum in Tibet, China, and evaluate the potential hazards of these pollutants for cultural relics. Long-term monitoring of indoor and outdoor suspended particulate matter concentrations was conducted, and sediment particle samples were collected. The chemical composition of the deposited particles was determined using X-ray fluorescence analysis (XRF). The outdoor suspended particulate concentration was much lower than that indoors; the indoor PM1-10 concentration was much higher than that outdoors and was less affected by outdoor sources. The sources of indoor deposited particles in the high-plateau museum can be classified into four categories: soil dust brought in by tourists from the outdoors, incense ash, pollution from human activities, and ores. Based on data analysis and discussion, proper ventilation can dilute indoor suspended particulate matter, and the installation of air conditioning systems can control temperature and humidity at 20 °C and about 45–60%, respectively, and reduce the fluctuation value, so as to promote particle deposition and better protect the museum’s cultural relics.
Pre-Clinical Models to Study Human Prostate Cancer
Authors: Thomsen Martin K. , Busk Morten
Published: August 2023

Prostate cancer is a common cancer among men and typically progresses slowly for several decades before becoming aggressive and spreading to other organs, leaving few treatment options. While large animals have been studied, the dog’s prostate is anatomically similar to humans and has been used to study spontaneous prostate cancer. However, most research currently focuses on the mouse as a model organism due to the ability to genetically modify their prostatic tissues for molecular analysis. One milestone in this research was the identification of the prostate-specific promoter Probasin, which allowed for the prostate-specific expression of transgenes. This has led to the generation of mice with aggressive prostatic tumors through overexpression of the SV40 oncogene. The Probasin promoter is also used to drive Cre expression and has allowed researchers to generate prostate-specific loss-of-function studies. Another landmark moment in the process of modeling prostate cancer in mice was the orthoptic delivery of viral particles. This technology allows the selective overexpression of oncogenes from lentivirus or the use of CRISPR to generate complex loss-of-function studies. These genetically modified models are complemented by classical xenografts of human prostate tumor cells in immune-deficient mice. Overall, pre-clinical models have provided a portfolio of model systems to study and address complex mechanisms in prostate cancer for improved treatment options. This review will focus on the advances in each technique.
Recent Advances in Wearable Sensors for the Monitoring of Sweat: A Comprehensive Tendency Summary
Authors: Xing Zhe , Hui Jianan , Lin Bo , Wu Zhenhua , Mao Hongju
Published: August 2023

Sweat, as a biofluid that is easy to extract and contains a variety of biomarkers, can provide various types of physiological information for health monitoring. In recent years, research on wearable sensors for sweat sensing has been emerging continuously. Wearable sweat sensing will probably become an alternative method to traditional chemical analysis. This is due to its advantages of portability, non-invasiveness, comfort, and continuous monitoring. Since the inception of this research field, wearable sweat sensors have achieved significant development in terms of materials, structures, systems, and application directions. Research interests are gradually evolving from single biomarker detection to the pursuit of multi-channel, multi-modal system-level architecture. The analysis of physiological signals has also developed from single signal characterization to omics analysis using multiple physiological information sources. Based on the changes mentioned above, this paper mainly introduces the latest researches of wearable sweat sensors from the aspects of strategy, architecture, material, system, data processing, etc., and tries to summarize the trends of sweat sensors. Finally, this paper analyzes the challenges faced by the sensing platform and possible methods for optimization.
Synthesis of Photoluminescent Carbon Dots Using Hibiscus Tea Waste and Heteroatom Doping for Multi-Metal Ion Sensing: Applications in Cell imaging and Environmental Samples
Authors: Mohandoss Sonaimuthu , Ahmad Naushad , Velu Kuppu , Khan Mohammad , Palanisamy Subramanian , You SangGuan , Lee Yong
Published: August 2023

Novel photoluminescent carbon dots (CDs) were synthesized through a facile hydrothermal method using Hibiscus tea extract as a natural carbon source and boric acid as a boron source. The optical and physicochemical properties of the as-synthesized nitrogen- and boron-doped CDs (NB-CDs) were characterized using UV–Visible (UV–Vis), photoluminescence (PL) spectroscopy, Fourier-transform infrared (FTIR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and transmission electron microscopy (TEM). The as-synthesized NB-CDs showed spherical morphology of approximately 6.2 ± 0.5 nm with quantum yield (9.2%), high aqueous solubility, strong photo-stability, and excitation-dependent PL behavior. The obtained NB-CDs exhibited high stability over a wide pH range and high ionic strength. Additionally, NB-CDs exhibited PL enhancement response with excellent sensitivity toward multi-metal ions, including Ag+, Cd2+, and Cr3+ ions, with very low detection limits of 44.5, 164.4, and 54.6 nM, respectively, with a wide concentration range of 0–10 μM. Upon testing the cytotoxicity of the NB-CDs at a concentration of 20 μg/mL for 24 h, we found no obvious inhibition of cell viability. Therefore, the proposed sensor method can be successfully applied to detect Ag+, Cd2+, and Cr3+ ions in cell imaging as well as in real water environmental samples.
Ultrasonographic Evaluation of Gastric Content and Volume in Pediatric Patients Undergoing Elective Surgery: A Prospective Observational Study
Authors: Demirel Asiye , Özgünay Şeyda Efsun , Eminoğlu Şermin , Balkaya Ayşe Neslihan , Onur Tuğba , Kılıçarslan Nermin , Gamlı Mehmet
Published: August 2023

Anesthesia-related complications, such as pulmonary aspiration of gastric contents, occur in approximately 0.02–0.1% of elective pediatric surgeries. Aspiration risk can be reliably assessed by ultrasound examination of the gastric antrum, making it an essential non-invasive bedside tool. In this prospective observational study, since most of our patients are immigrants and have communication problems, we wanted to investigate gastric contents and the occurrence of “high risk stomach” in children undergoing elective surgery for the possibility of pulmonary aspiration, even if the children and/or parents reported their last oral intake time. This risk is defined by ultrasound findings of solid content in the antrum and/or a calculated gastric volume exceeding 1.25 mL/kg. Children aged 2–18 were included in the study. Both supine and right lateral decubitus (RLD) ultrasound examinations were performed on the antrum before surgery. Using a qualitative grading scale from 0 to 2, we evaluated the gastric fluid content. The cross-sectional area (CSA) of the antrum was measured in the RLD position, aiding the calculation of the gastric fluid volume according to an established formula by Perlas. Ultrasound measurements of 97 children were evaluated. The median fasting duration was 4 h for liquids and 9 h for thick liquids and solids. Solid content was absent in all the children. Five children (5.2%) exhibited a grade 2 antrum, implying that fluid content was visible in both the supine and RLD positions. The median antral CSA in the RLD was 2.36 cm2, with a median gastric volume of 0.46 mL/kg. For patients with a grade 0 antrum, a moderate and positive correlation was observed between the antral CSA and BMI, and a strong and positive correlation was evident between the antral CSA and age, similar to a grade 1 antrum. Only a single child (1%) had a potentially elevated risk of aspiration of gastric contents. Hence, the occurrence of a “high risk stomach” was 1% (95% confidence interval: 0.1–4.7%) and is consistent with the literature. As a necessary precaution, we propose the regular use of ultrasound evaluations of gastric contents, given their non-invasive, bedside-friendly, and straightforward implementation, for identifying risks when fasting times are uncertain and for ruling out unknown risk factors in each potential patient.
Estimating the Risk of Attention Deficit Hyperactivity Disorder (ADHD) in Parents of Children with ADHD and the Association with Their Children’s Disease Severity and Adherence to Medication
Authors: Ragadran Jananheendaran , Kamal Nor Norazlin , Ismail Juriza , Ong Jun Jean , Sundaraj Charlotte
Published: August 2023

Background: Attention deficit hyperactivity disorder (ADHD) is characterised by inattentiveness, hyperactivity, and impulsivity. Up to half of the affected children have a parent with ADHD. In this study, the risk of ADHD among parents of ADHD children was estimated. The associations between parental ADHD and child ADHD severity and medication adherence were determined. Methodology: Parents of children and adolescents diagnosed with ADHD attending the University Kebangsaan Malaysia Medical Centre (UKMMC) were recruited between June to August 2022 and the administered Conners’ Adult ADHD Rating Scale (CAARS) self-report short form, Vanderbilt ADHD Parent Rating Scale (performance section), and Medication Adherence Report Scale (MARS). Results: Forty-five children with ADHD were recruited and 15 out of 45 (33%) parents were detected to have ADHD. ADHD severity was worse in children with ADHD parents for total severity (mean of 34.67 vs. 29.13, p = 0.047) and difficult behaviours at home (mean of 7.87 vs. 6.27, p = 0.036). The children’s academic performance and behavioural challenges at home and school were positively correlated with the parental ADHD scores for ‘inattention’ and ‘problems with self-care’ subscales. Conclusions: A total of 33% of ADHD children had parents with ADHD. ADHD children with ADHD parents were more likely to have behavioural problems at home and more severe ADHD. However, no statistical significance was noted with medication adherence.
Recurrent Sigmoid Volvulus in Children—Our Experience and Systematic Review of the Current Literature
Authors: Hencke Jonathan , Loff Steffan
Published: August 2023

Sigmoid volvulus (SV) occurs rarely in children. After encountering two cases of recurrent SV, we reviewed the literature to define the recurrence risk, identify outcome predictors and to give treatment recommendations. We found 256 cases reported in children (mean age 10.2 years, gender ratio (m:f) 2.3:1). Associations exist with Hirschsprung disease (HD) in 10%, neurodevelopmental disorders in 10.9% and chronic constipation in 10.2%. Common symptoms and clinical signs were abdominal pain (85%), distension (85%), tenderness (54%) and vomiting (59%). Signs of peritonitis were present in 14% and indicated a gangrenous sigmoid (X2 = 45.33; p < 0.001). A total of 183 had abdominal radiographs, and 65% showed a positive ‘coffee-bean-sign’. Contrast enemas were positive in 90%. A total of 124 patients underwent laparotomy; in 41 cases, the sigmoid was gangrenous and associated with more complications (X2 = 15.68; p < 0.001). Non-operative treatment (NOT) like endoscopic, fluoroscopic or rectal tube decompression was performed in 135 patients and successful in 79% with a 38–57% recurrence rate. A total of 73 patients subjected to elective surgery: 50 underwent sigmoid resection; 17 had surgery for HD. Clinicians should consider SV in all children with abdominal pain, distension and vomiting. Gangrene carries a higher morbidity. After successful NOT we recommend counselling about the recurrence risk and definitive surgery should be advised. HD is frequent in newborns but sometimes found in older children.
Dietary Pattern Indicators among Healthy and Unhealthy Weight Adolescents Residing in Different Contexts across the Otago Region, New Zealand
Authors: Coppell Kirsten J. , Keall Michael , Mandic Sandra
Published: August 2023

Reported obesity rates for adolescents in different urban and rural areas are inconsistent. We examined indicators of healthy and unhealthy dietary patterns among 1863 adolescents aged 13–18 years with a healthy or excess body weight attending 23 secondary schools in four different settlement types across the Otago region, New Zealand. An online survey included demographics and dietary behaviours. Height and weight were measured, and body mass index was calculated. New Zealand defined urban and rural settlement types were used. Home addresses determined a small area-level index of deprivation. Data were analysed using Chi-square tests and ANOVA. A logistic model was fitted to estimate adjusted odds ratios of excess weight. The proportion of adolescents with a healthy weight differed (p < 0.001) between the most (64.9%) and least (76.4%) deprived neighbourhood areas. There was only indicative evidence of differences between settlement types (p = 0.087). Sugar-sweetened beverage and fast-food consumption was more frequent in the most deprived areas (p < 0.001), and in urban versus rural settlements (p < 0.001). The most important associations with excess weight were area-level deprivation and ethnicity, but not settlement type. Prioritising socioeconomic factors irrespective of settlement type is necessary when developing interventions to improve dietary patterns and body weight status among adolescents.
The Chemosensing Role of CatSper in Mammalian Sperm: An Updated Review
Authors: Ke Sulun , Luo Tao
Published: August 2023

After sperm enter the female reproductive tract, the physicochemical and biochemical microenvironment undergoes significant changes. In particular, the large changes in various ions encountered by sperm may alter the physiology of sperm, ultimately compromising capacitation and fertilization. Thus, the rapid response to environmental variations is vital for sperm functions. For example, Calcium, the most crucial ion for sperm functions, enters into sperm via Ca2+ permeable ion channels. The cation channel of sperm (CatSper) is a sperm-specific, pH-sensitive, and Ca2+-permeable ion channel. It is responsible for the predominant Ca2+ entry in mammalian sperm and is involved in nearly every event of sperm to acquire fertilizing capability. In addition, CatSper also serves as a pivotal polymodal chemosensor in mammalian sperm by responding to multiple chemical cues. Physiological chemicals (such as progesterone, prostaglandins, β-defensins, and odorants) provoke Ca2+ entry into sperm by activating CatSper and thus triggering sperm functions. Additionally, synthetic and natural chemicals (such as medicines, endocrine disrupting chemicals, drugs of abuse, and antioxidants) affect sperm functions by regulating CatSper-dependent Ca2+ signaling. Therefore, understanding the interactions between CatSper and extracellular ligands sheds light on the mechanisms underlying male infertility and offers innovative diagnostic and treatment approaches. This underscores the importance of CatSper as a crucial regulatory target in male reproduction, linking sperm function with the extracellular environment. In conclusion, this review comprehensively summarizes the relevant studies describing the environmental factors that affect CatSper in humans and rodents.
Implementing Agile Data Workflows to Unlock Climate-Resilient Urban Planning
Authors: Vögt Verena , Harrs Jan-Albrecht , Reinhart Vanessa , Hollenbach Pia , Bühler Michael Max , Tewes Tim
Published: August 2023

Cities around the world are facing the implications of a changing climate as an increasingly pressing issue. The negative effects of climate change are already being felt today. Therefore, adaptation to these changes is a mission that every city must master. Leading practices worldwide demonstrate various urban efforts on climate change adaptation (CCA) which are already underway. Above all, the integration of climate data, remote sensing, and in situ data is key to a successful and measurable adaptation strategy. Furthermore, these data can act as a timely decision support tool for municipalities to develop an adaptation strategy, decide which actions to prioritize, and gain the necessary buy-in from local policymakers. The implementation of agile data workflows can facilitate the integration of climate data into climate-resilient urban planning. Due to local specificities, (supra)national, regional, and municipal policies and (by) laws, as well as geographic and related climatic differences worldwide, there is no single path to climate-resilient urban planning. Agile data workflows can support interdepartmental collaboration and, therefore, need to be integrated into existing management processes and government structures. Agile management, which has its origins in software development, can be a way to break down traditional management practices, such as static waterfall models and sluggish stage-gate processes, and enable an increased level of flexibility and agility required when urgent. This paper presents the findings of an empirical case study conducted in cooperation with the City of Constance in southern Germany, which is pursuing a transdisciplinary and trans-sectoral co-development approach to make management processes more agile in the context of climate change adaptation. The aim is to present a possible way of integrating climate data into CCA planning by changing the management approach and implementing a toolbox for low-threshold access to climate data. The city administration, in collaboration with the University of Applied Sciences Constance, the Climate Service Center Germany (GERICS), and the University of Stuttgart, developed a co-creative and participatory project, CoKLIMAx, with the objective of integrating climate data into administrative processes in the form of a toolbox. One key element of CoKLIMAx is the involvement of the population, the city administration, and political decision-makers through targeted communication and regular feedback loops among all involved departments and stakeholder groups. Based on the results of a survey of 72 administrative staff members and a literature review on agile management in municipalities and city administrations, recommendations on a workflow and communication structure for cross-departmental strategies for resilient urban planning in the City of Constance were developed.
Spin Coating of Silica Nanocolloids on Mica: Self-Assembly of Two-Dimensional Colloid Crystal Structures and Thin Films
Authors: Walker John , Koutsos Vasileios
Published: August 2023

The viability of spin-coating methods for the self-assembly of 150 nm diameter silica nanocolloids into large crystal structures on mica was investigated using different colloidal concentrations, accelerations, and rotational speeds. The samples were imaged by atomic force microscopy (AFM) in intermittent contact mode. Low colloidal concentration led to a size-dependent ordering configuration. The largest nanocolloidal particles formed crystalline close-packed structures that were surrounded by increasingly smaller nanocolloids configured into more polycrystalline or amorphous formations. This phenomenon became increasingly suppressed by increasing colloidal concentration. Two dimensional-fast Fourier transform (2D-FFT) radially averaged profiles of the topography images revealed increasing interparticle spacing with increasing rotational acceleration, from close-packed structuring at low accelerations to increasingly spaced packing at high acceleration (>800 rpm/s). This behaviour is attributed to rapid liquid shedding from the increased acceleration. Analysis with radial distribution functions quantified the extent of ordering and revealed an optimum spin speed that caused the formation of large, highly crystalline structures. This optimum spin speed is governed by the relationship between the rotational speed and the liquid film thickness that affect the uniformity of the film and the magnitude of the capillary forces generated.
Cortesognoite, CaV2Si2O7(OH)2·H2O, a New Mineral from the Molinello Manganese Mine, Graveglia Valley, Italy
Authors: Ma Chi , Carbone Cristina , Belmonte Donato
Published: August 2023

Cortesognoite (IMA 2014-029), CaV2Si2O7(OH)2·H2O, is a new vanadium silicate mineral that occurs within V-bearing lawsonite in association with vanadiocarpholite, chalcocite, quartz, minor poppiite, roscoelite, vanadomalayaite and volborthite in a silicified wood hosted in Mn-ore-bearing metacherts from the Molinello manganese mine in the Graveglia Valley, Northern Apennines, Liguria, Italy. The mean chemical composition of type cortesognoite by electron probe microanalysis is (wt%) SiO2 34.33, V2O3 31.38, CaO 15.80, Al2O3 7.69, MnO 0.14, FeO 0.09, MgO 0.06, TiO2 0.02 and H2O 10.29, totaling 99.80, giving rise to an empirical formula of (Ca0.99Mn0.01)(V1.47Al0.53Mg0.01)Si2.00O7(OH)2·H2O. The end-member formula is CaV2Si2O7(OH)2·H2O. Cortesognoite has the Cmcm lawsonite structure with a = 5.85(1) Å, b = 8.79(1) Å, c = 13.13(1) Å, V = 675(1) Å3 and Z = 4 as revealed by electron back-scatter diffraction. The calculated density using the measured composition is 3.44 g/cm3. Cortesognoite is a secondary alteration phase, formed with V-bearing lawsonite by multi-stage hydrothermal processes that occurred in the silicified fossil wood. The mineral name is in honor of Luciano Cortesogno, professor of petrography at University of Genova, Italy.
Crystal Structure and Microwave Dielectric Property of xMgO-SiO2 (x = 1~2) System for 5G Applications
Authors: Wang Yan , Li Jiajing , Zhu Haipeng , Wang Qilei , Sun Tulai , Ni Tao , Lin Yanghong , Liu Yu , Mao Minmin , Hu Ji , Liu Bing , Bafrooei Hadi , Ma Zhongyan , Ren Yingjie , Shi Feng , Taheri-Nassaj Ehsan , Wang Dawei , Song Kaixin
Published: August 2023

Mg2SiO4 and MgSiO3 ceramics with superior microwave dielectric properties are considered to be promising candidates for 5G applications. However, a slight deviation from the stoichiometric Mg/Si ratio will significantly influence their microwave dielectric properties, which will hinder their practical applications. In this work, the xMgO-SiO2 (x = 1~2) ceramics were synthesized by a solid-state reaction method. The influence of the Mg/Si ratio x on the crystalline phase, microstructure, and microwave dielectric properties was investigated through X-ray diffraction (XRD), a scanning electron microscope (SEM), and the resonant cavity method. The XRD patterns revealed the coexistence of Mg2SiO4 and MgSiO3 within the x range of 1~2, which was further demonstrated by the energy-dispersive X-ray spectra. The SEM images show a typical polycrystalline morphology of ceramics with an inhomogeneous grain size distribution. It is found that the microwave dielectric properties fluctuate at both sides of the x range while those remain relatively stable with minor changes at the intermediate components, indicating an obvious low composition dependence helpful for practical applications. Further, a demonstrator of a microstrip patch antenna for 5G applications using the 1.5MgO-SiO2 ceramic was designed and fabricated, and a return loss of −16.2 dB was demonstrated, which demonstrated the potential applications.
Morphometric Analysis of the Critically Endangered Fan Mussel (Pinna nobilis L.) in Maliakos Gulf (Central Aegean)
Authors: Tsamadias Ioannis E. , Rizou Dimitra D. , Moutopoulos Dimitrios K. , Lattos Athanasios , Giantsis Ioannis A. , Michaelidis Basile , Theodorou John A.
Published: August 2023

In this study, we present the relationships between total length, unburied length, and shell width and between total length and net weight for the critically endangered Pinna nobilis. This is the first transplantation study in which live specimens of P. nobilis have been used for estimating the length–weight relationship by deploying the unburied length. Length–length relationships were all linear for all cases (r2 > 0.900), whereas the length–weight relationship was negative allometric with the values of the exponent b ranging from 2.159 to 2.828. These relationships are important because they offer a restorative monitoring tool without damaging or sacrificing this endangered species, as total length can be computed using unburied length. By examining the relationships between different size dimensions in this re-allocated population, the present study also provided valuable insights for comparative growth studies, stock assessment models, and conservation purposes.