bluuebunny/crossref_metadata_2025 huggingface dataset:
Expression and Purification of SARS-CoV-2 Main Protease (Nonstructural Protein 5, NSP5) v1
Authors: Varela Kaitlyn
Published: October 2023

SARS-CoV-2 is the virus that caused the global COVID-19 pandemic in 2020-2023. This protocol describes how to express and purify the main protease of SARS-CoV-2 (nonstructural protein 5, NSP5) with a C-terminal hexahistidine tag. SARS-CoV-2 main protease is the enzyme (protein) consisting of 306 amino acid residues, responsible for the release of other important proteins in the virus' life cycle (i.e. NSPs 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16), including RNA polymerase (NSP12), which copies the virus' genome in the host. The inhibition of SARS-CoV-2 main protease activity is a strategy to kill the virus. The purified protein in this protocol was used to biochemically characterize SARS-CoV-2 main protease and identify a small molecule inhibitor (i.e. arteannuin B) in a previously published manuscript from our research lab: Varela, K.; Arman, H.D.; Berger, M.S.; Sponsel, V.M.; Lin, C.-H.A.; Yoshimoto, F.K. Inhibition of Cysteine Proteases via Thiol Michael Addition Explains the Anti-SARS-CoV-2 and Bioactive Properties of Arteannuin B. J. Nat. Prod. 2023, 86, 1654-1666. The purified protein was determined to be active through an assay using a model peptide substrate (4-fluoro-FLQS sequence). The peptide product (4-fluoro-FL) was detected using liquid chromatography mass spectrometry. The protease activity was inhibited with arteannuin B, a natural product isolated from the plant, Artemisia annua. Arteannuin B blocked the activity of the enzyme by forming a covalent bond with the active site cysteine residue of SARS-CoV-2 main protease (cysteine-145). Covalent bond formation was determined by mass spectrometry.
Verificationguidelines for the DDNS method for poliovirus direct detection v1
Authors: Akello Joyce
Published: October 2023

This document describes a pragmatic approach to conduct in-house verification of the DDNS method for detection of polioviruses. A laboratory should conduct an in-house verification on the entire DDNS method to confirm that the required performance characteristics can be met within the settings. This can prove that the laboratory can adequately perform the method as intended achieving the DDNS performance requirements for the sample matrix (stool) to which the DDNS method is applied for. Verification of the DDNSmethod will be assessed based on the following parameters: accuracy and sensitivity To verify the DDNS method performance, the laboratory should ensure that the lab personnel are adequately trained, competent,and qualified to perform the DDNS method. It is the responsibility of each laboratory to ensure that staff have attended DDNS training, biosafety risk assessment is performed, and adequate protective equipment is available to staff. Note: We recommend working closely with members of the Polio Sequencing Consortium (PSC) before and during the verification of the DDNS method to ensure that the laboratory has all the required components and competency to perform the full protocol.
Estimation of uncertainty in calculations of apparent iron solubility in seawater v2
Authors: Zhu Kechen
Published: October 2023

The apparent iron (Fe) solubility (SFe(III)app) was calculated via an ion paring-organic matter (NICA-Donnan) model at ambient pH, temperature and dissolved organic carbon (DOC). It suggests vertical distributions of dissolved Fe (DFe) were likely a function of SFe(III)appwith changes driven by pH, temperature and DOC, in addition to non-equilibrium processes such as scavenging and redox cycling. It is necessary to constrain the uncertainty in the calculations that result from uncertainties in model parameters, since few sets of model parameters describing the acid-base properties of marine dissolved organic matter (DOM). Here we proposed an efficient methodology by ORCHESTRA-PEST++ to conduct both uncertainty and sensitivity analysis.
A novel laboratory method to simulate climatic stress with successful application to experiments with medically relevant ticks v2
Authors: Hyo Kim Sang
Published: October 2023

This protocol details a novel method to isolate individual ticks and manipulate their environment. We successfully used this method to investigate how humidity affects survival and host-seeking (questing) behavior of three species of ticks: the lone star tick (Amblyomma americanum), American dog tick (Dermacentor variabilis), and black-legged tick (Ixodes scapularis). We placed 72 adult females of each species into individual plastic tubes and separated them into three experimental relative humidity (RH) treatments representing distinct climates: 32% RH, 58% RH, and 84% RH. For 30 days we assessed the survival and questing behavior of each tick. The last step in this version contains a supplemental video with extra context and tips, as part of the protocols.io Spotlight series, featuring conversations with protocol authors.
Image processing and 3D reconstruction v1
Authors: Chen Minghao
Published: October 2023

Image processing and 3D reconstruction
Self-Checking Combinational Devices Synthesis Based on the Boolean Signal Correction Method Using Bose—Lin Codes
Authors:   Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Efanov D. V. ,   Russian University of Transport, Moscow,,   Tashkent State Transport University, Tashkent, Uzbekistan,   Scientific Research and Design Institute "Transport and Construction Safety" LLC, St. Petersburg
Published: October 2023

An organizing concurrent error-detection (CED) circuit new method for automation and computer technology combinational device is considered. The method based on the signal's Boolean correction method. The author proposed to use separable codes in the CED circuit synthesis when converting values from all operational outputs of the diagnosing object. The proposed approach allows to consider many possible transformations for each input combination. In addition, it will allow to choose the transformations to provide the conditions for building a completely self-checking device. In comparison with the methods previously discussed in the literature, the proposed approach makes it possible to synthesize a large number of control logic devices in the CED circuit and choose the most appropriate option. For example, with the technical implementation complexity lowest indicators. An algorithm for synthesizing the control logic block is given on the example of using a separable Bose — Lin code with module M = 4 by the signals Boolean correction method when organizing an CED circuit. The proposed method advantages and disadvantages for the CED circuit synthesis are described.
Approach to Generating Data for Analytical Reporting in the Enterprise Management System
Authors:   Academician M. F. Reshetnev Information Satellite Systems, Kartamyshev A. S. , Chernysh B. A. ,   Academician M. F. Reshetnev Information Satellite Systems
Published: October 2023

Analytical reporting tools allow to turn disparate data accumulated in the information system into structured information described in terms of the relevant subject area. This information makes it possible to timely and adequately respond to changes, make informed management decisions for business leaders, analysts, economists, etc. The article describes the composition of business analysis systems, the components of which are built using OLAP technology. Varieties of OLAP solutions are considered. A method for constructing multidimensional cubes is proposed, developed and implemented by the authors in an automated system for managing financial and economic tasks. The scheme of storage, methodology and algorithm of data processing are described on the example of the "Purchases" subsystem. Examples of real analytical problems solved during the operation of the system at an industrial enterprise are given. A comparative analysis of data sampling time measurements based on structures and algorithms that provide the described approach to data generation, with a sample from unprepared relational tables for real problems from examples, was carried out. The texts of the corresponding SQL queries are given. The measurement results are summarized in tables and visualized in the form of a diagram. The evaluation of the results obtained is carried out, the problems of using unprepared data are described. The article also discusses the features of the analytical mechanisms of commercial accounting systems on the example of the 1C: Enterprise 8.3 platform (registers of accumulation, information, accounting, calculations). The effectiveness of the considered mechanisms for the formation of analytical reporting was assessed in comparison with the proposed approach to data structuring.
Martin Heidegger: reflexes of the picture of the world (be continued)
Authors: Kostetckii Victor V.
Published: October 2023

The article focuses on the philosophical analysis of the concept of time. Thanks to M. Haidegger, time is interpreted outside the attitude to movement and three forms of time (present, past, future). The authors position boils down to the fact that time scales being, thereby representing different paintings of the world and different ontologies. In terms of their ontological status, the concepts of time and knowledge intersect. As for the traditional ratios of time and movement, this ratio is of a particular nature, significant in the everyday scaling of time.
Features of Adaptation of Indigenous Students of the Russian North, Siberia and the Far East to the College Educational Environment
Authors: Baeva I.A. , Miklyaeva A.V. , Pezhemskaya J.S. , Khoroshikh V.V.
Published: October 2023

Objective. Empirical study of the criteria for assessing the adaptation of students – representatives of the indigenous and minorities of the North, Siberia and Far East to the educational environment of colleges and the study of the features of their adaptation.
Background. The relevance of studying the features of adaptation of students – representatives of the indigenous and minorities of the North, Siberia and the Far East of the Russian Federation to the educational environment of colleges is due to the high importance of secondary vocational education for the preservation and development of the culture of these peoples.
Study design. Factor analysis made it possible to identify indicators of students' adaptation to the educational environment of the college (adaptation criteria). To clarify the features of the adaptability of students-representatives of the SIM and Far East and the role of environmental factors mediating adaptation, correlation, variance analysis, as well as analysis of the significance of differences were used.
Participants. Students-representatives of the of the indigenous and minorities of the North, Siberia and the Far East of the Russian Federation – 304 people (179 girls, 125 boys). Students of the comparison group – 416 people (195 girls, 221 boys).
Measurements. Russian-language version of the Scale of Sociocultural Adaptation J. Wilson', K. Ward', et al. The author's scale of self-assessment of the degree of satisfaction with the main aspects of life. Author's questionnaire for assessing the degree of adaptation to an educational organization.
Results. Three groups of indicators of students' adaptability to the educational environment of the college are identified: academic adaptation (23% of the variance), sociocultural adaptation (13%) and psychological adaptation (13%). Indigenous and minority students and comparison groups do not differ in terms of socio-cultural and psychological adaptation, while indicators of academic adaptation of students – representatives of the indigenous and minorities of the North, Siberia and the Far East of the Russian Federation are higher than in the comparison group in the conditions of the homogeneity of the socio-cultural environment of the college and lower in the conditions of its heterogeneity.
Conclusions. The indicators for assessing the adaptability of students are independent of each other and allow assessing the features of adaptation to an educational organization, educational activities and group; features of adaptation to a new socio-cultural environment, as well as the degree of emotional well-being of the individual.
Psychodiagnostics of Value Orientations of Adolescents: the Results of Standardization of the Methodology "Hierarchy of Life Values" by G.V. Rezapkina
Authors: Ulyanina O.A. , Radchikova N.P. , Borisenko E.V. , Khayrova Z.R.
Published: October 2023

Objective of the study is to check the psychometric properties and validity of the questionnaire “Hierarchy of Life Values” (G.V. Rezapkina), which allows to determine the preferences of eight basic values (Health, Welfare, Creativity, Family, Career, Service, Glory, Recreation) for adolescents aged 10-18.
Background. At the moment, the values of a teenager are formed in a situation of social instability and value-normative uncertainty, so the task of adolescents’ values study is of great importance, imposing special requirements on the choice of research tools.
Study design. The survey was conducted online using Google forms. The questionaries “Life Values Hierarchy” (G.V. Rezapkina), “Purpose-In-Life Orientations” (D.A. Leontiev) and “Questionnaire for the study of emotional attitude to learning” (A.M. Prihozhan) were used.
Participants. The study included 1303 adolescents aged 10 to 18 years (mean age = 13,7 ± 1,7 years, median = 13 years); of these, 42% were female, 32% were male, and 26% did not indicate their sex. Measurements. Cronbach's alpha and MacDonald's omega, exploratory and confirmatory factor analyzes were used to test the internal consistency of the questionnaire; Pearson's correlation coefficient was used to check external validity; three-way ANOVA with Duncan's posterior test was used to determine gender and age differences; and multidimensional scaling was used to determine the space of life values.
Results. The results showed a fairly good internal consistency of all scales (Cronbach's α = 0,75-0,84), while exploratory and confirmatory factor analyzes confirm the 8-factor structure of the questionnaire. External validity is confirmed by relationships with purpose-in-life orientations, motivation and emotional attitude to learning. Gender and age differences are small. Girls value creativity and glory more than boys. As adolescents age, the value of a career and material security increases, while the value of service decreases. Conclusions. The questionnaire “Life Values Hierarchy” (G.V. Rezapkina) is simple, effective, reliable and valid and can be used both for research and applied purposes for adolescents aged 10-18.
State of cancer care in Russia: Age and cancer. Features of localization structure, quality of recording, and survival of middle-aged patients with CNS tumors (a clinical-population study). Part 3.
Authors: Merabishvili Vakhtang M. , Shakhzadova Anna O. , Perelygin Vladimir V.
Published: October 2023

According to the World Health Organization (WHO) standard, the middle age refers to the population between 45 and 59 years old. In the previous two studies, we examined the specific characteristics of the detailed localization structure of morbidity, mortality, and survival of children, adolescents, and young adults. This analysis of these characteristics for individuals belonging to the middle age group is conducted for the first time. The population belonging to this age group is most actively involved in public life, has a stable financial status, and often finds themselves at the peak of their professional and personal maturity. The concept of "middle age" encompasses three components. According to the WHO standards, the first component represents the total population aged 45 to 49 years. In the previous two studies, we examined the characteristics of the detailed localization structure of morbidity, mortality, and survival of children, adolescents, and young adults. The analysis of these characteristics for individuals belonging to the middle age group is conducted for the first time. The population that falls within this age group is most actively involved in public life, has a stable socioeconomic status, and often reaches the pinnacle of their professional and personal maturity. The concept of "middle age" encompasses three components. According to the standards of the World Health Organization (WHO), the first component represents the aggregate population aged 45 to 49 years.
Multi-omics Characterization of the Loss of Transcriptomic Modulators SOX10 and BAP1 in Cutaneous and Uveal Melanomas
Authors:   , Purwin Timothy J. , Aplin Andrew E , Sacan Ahmet
Published: October 2023

Identifying alterations to the transcriptomic networks in cancers is crucial for studying their mechanisms and exploiting their weaknesses. In cutaneous melanoma (CM), the deadliest form of skin cancer, and uveal melanoma (UM), the most common type of intraocular cancer, loss of specific transcriptomic modulators significantly impacts the regulation of these networks and is associated with disease progression. In CM, recent research into transcriptomics at the cellular level has revealed that cell state plasticity plays a major role in therapeutic resistance. Loss of SOX10 transcription factor activity has been linked to drug tolerance and an invasive phenotype. Its involvement with immunotherapy resistance is currently unknown. Identifying gene targets and determining its activity in transcriptomic data are important for studying its role in disease progression. In UM, the median overall survival time for a patient with metastatic disease is between six months and a year. Loss-of-function mutations in BAP1, an epigenetic regulator that deubiquitinates monoubiquitinated Histone H2A, occur in 45% of primary-site tumors. Despite this, 80% of metastatic tumors present genomic loss of BAP1. The goals of this thesis are to characterize a transcriptome profile due to SOX10 loss in CM and to identify transcriptomic targets linked to disease progression due to BAP1 loss in UM. By integrating multiple datasets, layers of 'omics data, and gene-dependent results with patient-derived data, we identified genes core to the loss of SOX10 in CM and BAP1 in UM. We developed a robust SOX10 loss regulatory gene signature by integrating SOX10 ChIP-seq with time-course knockdown ATAC-seq and CRISPR knockout RNA-seq data. We validate the gene signature using multiple SOX10 knockdown, cell line panel, and drug-resistant gene expression datasets. We determine if SOX10 activity is lower in immune checkpoint inhibitor-resistant tumors using patient-derived single-cell RNA-seq data. We use similar methods to reveal BAP1 dependent cell migration and invasion pathway genes in UM. Comparisons between BAP1 mutant and wild-type patient tumors, cell line panels, re-expression, and knockdown datasets reveal consistent changes in gene expression. Patient-derived single-cell RNA-seq data show a selective expression of these genes in malignant cells, and reverse-phase protein array data confirm these differences at the protein level. Genes and mechanisms central to the loss of SOX10 and BAP1 activities identified in this study will enable further investigation into disease progression and discovery of targets for therapeutic intervention in CM and UM.
Impact of Enhanced External Counter-pulsation Therapy on Glycaemic Control in People With Prediabetes and Type 2 Diabetes Mellitus: A Systematic Review and Meta-analysis
Authors: Nagendra Lakshmi ,   Department of Endocrinology, JSS Academy of Higher Education and Research, Mysore, India, Dutta Deep ,   Department of Endocrinology, Center for Endocrinology Diabetes Arthritis & Rheumatism (CEDAR) Superspeciality Healthcare, Dwarka, New Delhi, India, Sharma Meha ,   Department of Rheumatology, Center for Endocrinology Diabetes Arthritis & Rheumatism (CEDAR) Superspeciality Healthcare, Dwarka, New Delhi, India;, Bg Harish ,   Department of Anaesthesiology, JSS Academy of Higher Education and Research, Mysore, India
Published: October 2023

Background: Enhanced external counter-pulsation (EECP) therapy is approved for refractory angina in coronary artery disease (CAD). EECP is being explored as a treatment modality in type 2 diabetes mellitus (T2DM). Methods: The Embase, Web of Science, Cochrane Library, MEDLINE (PubMed), ClinicaltTrials. gov, CNKI database, Clinical Trials Registry-India (CTRI), and Google Scholar databases were searched for randomized controlled trials (RCTs) involving patients receiving EECP therapy in the intervention arm. The primary outcome was the changes in glycated haemoglobin (HbA1c). The secondary outcomes were the changes in blood glucose parameters, inflammatory markers and any adverse events. Results: Data from 3 RCTs involving 71 people with T2DM/prediabetes was analysed to find out the impact of EECP therapy compared with placebo. As compared with placebo, patients receiving EECP had significantly lower HbA1C immediately after completion of therapy (mean difference [MD] -0.70%, 95% confidence interval (CI) -0.95. -0.45;p<0.00001), at 2–4 weeks post completion of therapy (MD -1.04%, 95%CI -1.32. -0.77; p<0.00001) and 7–12 weeks after therapy completion (MD -0.98%, 95% CI -1.22, -0.74; p<0.00001). EECP therapy was well tolerated without any increased side effects (risk ratio 2.36, 95% CI 0.11–52.41; p=0.59. Conclusion: EECP therapy is effective in blood glucose and pressure lowering over at least 7–12 weeks of therapy completion. Blood glucose and pressure should be monitored with suitable modulation of drug doses to prevent hypoglycaemia and hypotension in patients with angina undergoing EECP therapy. The PROSPERO registration number is CRD42023434533
Pharmacologic Mydriasis and Cycloplegia: A Review of Novel Delivery Devices
Authors: Jordan Jalin A ,   Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA, Oatts Julius T ,   Department of Ophthalmology, University of California, San Francisco, CA, USA
Published: October 2023

Pharmacologic mydriasis, or pupillary dilation, is achieved using various agents, and is an important component of a comprehensive eye examination. Of the medications available to achieve mydriasis, the most favourable are those with a rapid onset and a low risk of causing side effects. The current standard of care is to administer multiple drops of different medications to achieve mydriasis. However, a number of innovations in the field are addressing this shortcoming. Fixed-combination eyedrop therapy has been suggested as a more efficient method, with the potential to cause fewer side effects. Additionally, novel drug delivery devices have been devised to further improve efficiency and decrease waste by delivering smaller volume drops with greater precision. Two devices of current interest are the Nanodropper (Nanodropper, Inc., Rochester, MN, USA) and Optejet® (MydCombi™; Eyenovia, Inc., New York, NY, USA). Recent studies of these devices have shown their ability to achieve similar levels of mydriasis as conventional medications without additional side effects. Furthermore, the Optejet has shown in two phase III trials that fixed-combination therapy is able to achieve greater pupil dilation compared with the current standard. This review provides an overview of pharmacologic dilation and reviews these emerging technologies.
The Excessive Carbon Footprint of Inhalers Used in Airway Disease and its Remedies
Authors: Désy Laurence ,   Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, QC, Canada, Lachapelle Philippe ,   Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, QC, Canada, Couillard Simon ,   Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, QC, Canada
Published: October 2023

The carbon footprint of human activities is a conversation topic worldwide. Many fields, including healthcare, have attempted to reduce their carbon footprint. For example, in Canada, 4.6% of greenhouse gases are manufactured by healthcare institutions. When it comes to the accountable parties in healthcare, the current treatments for asthma and chronic obstructive pulmonary disease are the main contributors. These highly prevalent diseases affect 11% and 10% of the Western populations, respectively. This editorial aims to discuss the impact of pressurized metered dose inhalers on the environment, alternatives and changes to current diagnostic and therapeutic practices, public awareness, potential changes to regulations and paths forward.
Desarrollo y simulación del subsistema de operación automático para trenes
Authors: Bilbao Moreno Daniel , Elorza Iker , Irigoyen Eloy
Published: October 2023

Con objeto de mejorar la calidad del servicio, aumentar la eficiencia y seguridad de la operación, y reducir los costes operativos y ambientales en los actuales sistemas de transporte ferroviario, este trabajo presenta el desarrollo del subsistema de operación automático para trenes (ATO, Automatic Train Operation). En particular, se enfoca en la capa mesoscópica, la cual hace referencia al planificador encargado de generar las señales de control a proporcionar a la capa microscópica, asociada al módulo motor. Mencionar que, aunque este desarrollo no aporta una novedad significativa, es un paso fundamental para futuras contribuciones innovadoras en esta línea de investigación. Por ello, se ha modelado el tren a partir de la dinámica longitudinal que lo caracteriza y se ha implementado un algoritmo inicial, encargado de planificar y regular la velocidad del tren hasta cumplir con las especificaciones impuestas por la capa macroscópica. La solución propuesta ha sido validada mediante simulaciones que incorporan diversas perturbaciones en la vía. El análisis de los resultados obtenidos permiten corroborar que el desarrollo cumple con el objetivo principal de manera satisfactoria, dando paso a la futura mejora del algoritmo mediante la implementación de técnicas de control inteligentes.
Unveiling the potential of barrier membranes in implant dentistry: A comprehensive review
Authors: Aeran Himanshu , Kumar Varun , Seth Jyotsna , Aeran Manas
Published: October 2023

Barrier membranes have emerged as crucial tools in guided tissue regeneration (GTR) and guided bone regeneration (GBR) procedures, specially transforming the landscape of implant dentistry. An overview of the key characteristics, types, and clinical implications of barrier membranes in implant dentistry has been described. These membranes create a controlled environment that facilitates tissue and bone regeneration around dental implants, enhancing treatment outcomes. The article delves into the ideal attributes of barrier membranes, including biocompatibility, mechanical stability, selective permeability, and more. It also explores various forms of barrier membranes, such as expanded polytetrafluoroethylene (ePTFE), collagen, and pericardium membranes, highlighting their unique advantages and considerations. The clinical applications, drawbacks, and future directions of these membranes are discussed, shedding light on their role in managing complex cases. The evolving landscape of advanced biomaterials, bioactive coatings, patient-specific approaches, and smart membranes points toward an exciting future for barrier membranes in implant dentistry, promising further advancements in guided regeneration techniques.
Parental education and attitude of young adults towards their oral health - A cross-sectional study
Authors: Krishnan Parvathy , Bhat Sreevidya
Published: October 2023

Socioeconomic factors are linked with the prevalence and burden of oral diseases, particularly in socially marginalized groups and developing countries. The Kerala health model shows the relevance of education and its direct link to attitudes towards health. We studied the relevance of the educational level of parents on the oral health attitude of 17–25-year-olds. The objective was to examine the relationship between parents’ education and oral health attitude, frequency of dental visits, self-rated oral health (SROH), refrainment from dental health care, awareness of follow-up treatment, and effect of female literacy on offspring’s oral health attitudes.

College students in Kerala, who were 17-25 year-old were administered questionnaires either web-based via Google forms or paper-and-pencil-based, among a randomly selected sample of college students. The questionnaire sought information on parents' educational levels, SROH, oral care services and awareness regarding oral health. Univariate and bivariate analysis with Chi-squared test was done using SPSS.

About 939 students participated in the survey. Among them, 60.7% were females and 39.3% were males and 84.5% were undergraduate and 15.5% were postgraduate students.

Frequency of visiting a dentist, SROH, visit to a dentist on recall, and necessary for follow-up and dental visit were associated with father’s and mother’s level of education. Level of education of parents was associated with off-spring’s perception of oral health, and dental visit factors.
Knowledge, awareness and attitude of parents on pulp therapy of primary teeth: A questionnaire study
Authors: Mahabaleshwara Pooja , Hegde K Sundeep , Sargod Sharan S , Rao HT Ajay
Published: October 2023

: Many of the parents are not aware that primary dentition should be maintained in the dental arch in a healthy state for the overall health of the child. They believe that the treatment of primary teeth is not necessary as they are replaced by permanent teeth.

: This study aimed to evaluate the knowledge, awareness and attitude of parents towards primary tooth pulp therapy.

: Pretested questionnaire which included the demographics like age, gender and education level with further investigative questions on the need for pulp therapy in primary teeth, knowledge about pulp treatment, importance of pulp treatment, reason of dental visit, restoration of carious tooth and preference of pulp therapy was offered to the participants. Data was collected and compiled using Excel software. Descriptive statistical analysis was performed to obtain the results.

: A total of 78 parents participated in the study. Out of which, 69 were female and 9 were men. Based on the education level, parents who studied below 10 standard felt that dental treatment was not needed for primary teeth and 62.5% of them felt that pulp therapy of primary teeth is not needed. On the other hand, parents who completed postgraduation knew that primary teeth are important and dental treatment is required for primary teeth. There is significant lack of awareness among parents regarding treatment of primary teeth.

This present study has strived to gather vital information on parents’ oral health awareness and attitudes towards their child’s decayed teeth. While some of the responses were valid, it was clear that parents are still unaware of some important aspects of their child's oral health.
Rehabilitation of the patient with ceramic laminate veneers having midline diastema and rotated maxillary central incisors associated with highly attached maxillary labial frenum- A case report
Authors: Raipure Pravin , Pawar Ravindra , Kharsan Vishwas
Published: October 2023

IPS Emax is a lithium disilicate glass ceramic that has excellent translucency, longetivity, durability and strength for all restorations. It is a unique system that fulfills all of your patient demands for minimally invasive dentistry. It is a choice for single anterior/posterior crowns, as it truly mimics the light refraction and natural translucency necessary for outstanding esthetic appearance. Maxillary midline diastema with associated rotation of maxillary incisors presents a very challenging situation for dentist to meet the esthetic requirements. The presence of highly attached labial frenum in such patients adds to the complexity of the outcome of the treatment. Labial frenectomy done with the Laser presents a very quick and early satisfactory healing with no postoperative patient discomfort. Presenting a one such case report of rehabilitation of maxillary midline diastema with the IPS Emax ceramic laminate veneers.
Antibiotic resistance and susceptibility pattern of different microorganisms against nadifloxacin
Authors: Gala Monil Yogesh Neena , Muchhala Snehal , Bhagat Seema , Sanghavi Arti , Rathod Rahul , Kotak Bhavesh , Khadapkar Rashmi
Published: October 2023

Skin and soft tissue infections (SSTIs) are common and can have serious implications.Nadifloxacin’s broad-spectrum antibiotic activity may potentially provide therapeutic benefits for skininfections. Also, it offers a viable alternative therapy for topical agent resistance.

This study investigates the antimicrobial susceptibility of eight micro-organisms to four important topical antibiotics: Mupirocin, Clindamycin, Fusidic acid, and Nadifloxacin.

Antibiotic susceptibility and minimum inhibitory concentration (MIC) were determined using Kirby-Bauer disk diffusion, Epsilometer test (E-Test), and Micro-broth dilution methods. Mueller Hinton and Brucella blood agar served as growth media. HiComb strips from HiMedia were used, and QC strains were tested. Kirby-Bauer assessed Zone of Inhibition; HiComb determined MIC via gradient; Micro-broth dilution gauged growth in antibiotic-diluted broth.

The disk diffusion method revealed varying resistance percentages for antibiotics. Clindamycin had the highest resistance (62%) followed by fusidic acid (47%), Nadifloxacin (15%), and mupirocin (5%). Among gram-positive isolates, Nadifloxacin and mupirocin had 100% sensitivity, while Fusidic acid showed moderate resistance (19%) and clindamycin highest resistance (42%). Among gram-negative isolates, clindamycin and fusidic acid had 100% resistance, while Nadifloxacin (42%) and mupirocin (15%) showed comparatively low resistance. Among the 57 species isolates, including 49 isolates of and 8 isolates of the antibiotic susceptibility testing revealed a MIC value <4 μg/ml of Nadifloxacin, with a high level of sensitivity across all isolates.

Nadifloxacin’s superior efficacy in the study can be attributed to its mechanism of action, targeting bacterial DNA gyrase and topoisomerase IV, making it suitable for bacterial infections, particularly those involving the skin and soft tissuesOut of four antibiotics tested, Nadifloxacin was found to be effective against both gram-positive and gram-negative strains of bacteria.
Proyectización y Dimensiones Homológicas
Authors: Bustamante Juan Carlos , Huard François , Smith David
Published: October 2023

Estudiamos propiedades homológicas del álgebra de endomorfismos EndΛ(P∞)op, donde Λ es un álgebra de artin de dimensión global infinita, y P∞ es la suma directa de los representantes de la clases de isomorfismo de los Λ-módulos proyectivos indescomponibles tales que P/rP tiene dimensión proyectiva infinita.
Health-related Quality of life in Patients on Hemodialysis Compared to Peritoneal Dialysis: A cross-sectional Study
Authors: Namdar Azam , Naghizadeh Mohammad Mehdi , Namdar Fatemeh , Alizadeh Samiyeh , Montazeri Ali
Published: October 2023

Background & Objective: Health-related quality of life (HRQoL) in patients with chronic renal diseases is an important factor for physicians to select a dialysis modality. The present study aimed to compare the HRQoL in hemodialysis (HD) and peritoneal dialysis (PD) patients.
Materials & Methods: In this cross-sectional study, a sample of HD patients and a sample of age- and sex-matched PD patients with at least six months dialysis were recruited. Then, the quality of life was assessed using the Short Form Health survey (SF-36). Demographic and disease-related information was also recorded. Then, data were analyzed using the SPSS software (ver. 21).
Results: Totally, 172 HD patients and 86 age- and sex-matched PD controls were entered into the study. Overall the PD patients had more dialysis per week (P < 0.001). Comparing quality of life between two groups showed significant differences in physical functioning, rolephysical, role-emotional and physical component summary observed (P < 0.05). In addition, role physical in HD patients and bodily pain in PD were the worst and best dimensions of quality of life, respectively.
Conclusion: The findings suggest that patients undergoing peritoneal dialysis had a better quality of life in most measures. It seems that we need to educate and encourage patients to choose peritoneal dialysis in order to enjoy a better quality of life.
The Correlation between Clumping Factor A Gene Expression in Biofilm Formation and Antibiotic Resistance Among Staphylococcus aureus Isolated from Urine Samples of Imam Khomeini Hospital, Tehran
Authors: Ghasem Azizi Leila , Ghasem Azizi Arash , Hemati Behzad , Ebadi Monier
Published: October 2023

Background & Objective: Staphylococcus aureus causes problems in hospitals and it has emerged as a serious agent acquired from the environment in recent years. One of the capabilities of S. aureus is the formation of biofilms, in which bacteria can exchange antibioticresistance genes among themselves and increase the virulence of other strains of this species (S. aureus). A surface protein attached to the cell wall in S. aureus clumping factor A is a virulence factor in various staphylococcal infections.
Materials & Methods: In this study, after the Urea Analysis (UA) test, the urea culture test was applied to the blood agar and Baird-Parker Agar culture media from the infectious urine samples in Imam Hospital, Tehran, to identify S. aureus isolates. Finally, a molecular method was used for the confirmation of identified isolates. The microliter plate method was performed to determine the biofilm formation ability. The disk diffusion method was also used for profiling the antibiotic resistance of the isolates.
Results: In the results of this study, 45 out of 160 urinary clinical samples were positive for S. aureus, among which 42 isolates expressed the clfA gene. Moreover, 39 isolates had the ability to form biofilms in vitro. Among these 42 isolates, the highest (88%) and the lowest (16%) rates of antibiotic resistance were observed against penicillin and cefoxitin, respectively. Data analysis with SPSS software and chi-square indicated a significant relationship between gene expression and biofilm production with antibiotic resistance (P < 0.05).
Conclusion: The resistance of S. aureus bacteria is increasing strongly due to the repeated use of antibiotics such as beta-lactams, especially in respiratory infections and pharyngitis. Moreover, biofilm formation and virulence factors, such as clfA and clfB, cause concerns to the World Health Organization for treatment, especially for people with sepsis or toxemia.
Comparison of manual lymphatic drainage massage and negative pressure massage therapy efficacy in lymphedema patients: a randomized controlled study
Authors: ERSOY Sedef , KESİKTAŞ Nur , ŞİRİN Büşra , BUĞDAYCI Nazlı Derya , KİBAR Halime , PAKER Nurdan
Published: October 2023

Objectives: Lower extremity lymphedema due to secondary causes is a lifelong complication that can be encountered. Its treatment is essential, because it has significant impact on quality of life and daily living activities related to lower extremity involvement. This research aims to compare the effects of Manual Lymphatic Drainage Massage (MLD) and Negative Pressure Massage Therapy (NPMT) treatments in order to provide maximum benefit to patients.
Methods: This prospective, randomized study included 30 patients with lower extremity lymphedema due to secondary causes. Patients, randomized using computer software, were divided into two groups. The first group (n =15) received 45 minutes, 15 sessions of MLD, while the second group (n =15) received 45 minutes of 15 sessions of NPMT using the LymphaTouch device. Compression bandaging was applied to both groups and self-drainage training was given to all patients. The circumference of the extremity at 6 reference points were measured and their pain and discomfort assessed by the Visual Analogue Scale (VAS) were recorded before and after treatment. Changes within the groups and between the groups were compared using the SPSS statistical program.
Results: Statistically significant improvement was observed in all parameters in both treatment groups. The decrease in VAS pain and VAS discomfort scores (p < 0.05 and p < 0.01; respectively), circumference measurement of the extremity (p < 0.01) was statistically greater in the NPMT group compared to the MLD group.
Conclusions: In conclusion, NPMT appears to be a beneficial non-invasive treatment method for reducing extremity volumes and decreasing subjective pain and discomfort in lymphedema patients.
Online Eye Movement Desensitization and Reprocessing Therapy for Chronic Pain: A Pilot Controlled Trial
Authors: Rosser Benjamin A. , Agostinis Alessio , Bond Jonathan
Published: October 2023

The study aimed to provide a preliminary evaluation of the acceptability and effectiveness of online eye movement desensitization and reprocessing (EMDR) compared with a waitlist control (WLC). A pilot nonrandomized controlled trial was conducted. Eighteen adults experiencing chronic pain completed the study (nEMDR= 10;ncontrol= 8). The intervention group received up to 10 weekly sessions of online EMDR. The control group received treatment as usual. Participants completed baseline and post-intervention measures assessing posttraumatic stress, pain severity, interference, and catastrophizing, and depression levels. Additionally, the online EMDR group participants provided feedback on intervention acceptability and satisfaction. The online EMDR group demonstrated significant reductions in both trauma and pain-related outcomes; depression levels did not significantly change. No significant change was observed in any outcome within the control group. After the WLC also received the intervention, additional analysis results demonstrated similar effects but did not reach statistical significance, except for depression. Overall, online EMDR appeared acceptable and positively received by participants. The study provides preliminary support that online delivery of EMDR may reduce trauma- and pain-related outcomes in individuals experiencing chronic pain. Further large-scale research is warranted to substantiate these findings. Limitations and implications are discussed. REC ref: 2020/HCSREC/04
Benchmarking the Performance of State Owned Power Distribution Utilities
Authors: Vishwakarma Dr. Suresh
Published: October 2023

One of the greatest challenges faced by developing nations today is to operate and manage their power industries in efficient and economical way. This study focuses on one aspect of economic performance, namely the efficiency with which electricity is generated. A non-parametric technique, Data Envelopment Analysis (DEA) is used to compare the performance of State Owned Power Distribution Utilities (SOPDU) with those other in similar stage of development. Technical efficiency of electric utility is the critical element for its competitiveness in the Power Market and very relevant in the Indian Power sector presently. This Study is aimed to measure the efficiencies of 16 SOPDU for the year 2011-12 by applying DEA models with single input and two outputs. The result of this model shows that SOPDU are generally inefficient in expenditure frontier and there is an urgent need for intro inspection. This will help for SOPDU. The result shows that the total average of overall, technical and scale efficiencies are 32.7%, 71.6% and 51% respectively. This efficiency measurement assists the utilities by identifying their shortcomings, setting targets and trying to reach the set targets.
Diabetes na população indígena adulta brasileira: uma revisão integrativa
Authors: Benedito Júnior Cesar de Souza , Medeiros Arthur Almeida , Sá Jennyfer Soares de , Teston Elen Ferraz
Published: October 2023

Os indígenas brasileiros, em contato com a população não indígena, apresentam em transição nutricional e epidemiológica que reflete na taxa de prevalência do diabetes mellitus desses povos originários. Este estudo objetiva analisar a produção científica sobre a prevalência de diabetes mellitus tipo 2 na população indígena adulta. A revisão integrativa foi elaborada conforme o relatório de pesquisa PRISMA, considerando um recorte temporal entre os anos de 2011 e 2022 nas bases de dados da LILACS, CINAHL, Scopus, Embase e PubMed/MEDLINE. Identificaram-se 319 artigos, dos quais 14 atenderam aos critérios de inclusão. A prevalência de diabetes entre indígenas adultos variou de 3,0% a 24,9%, com média de 10,5%. Em suma, foi observada uma grande variabilidade na prevalência entre indígenas brasileiros, sendo aproximada da população em geral. Portanto, sugerem-se novos estudos com foco nesta população, para conhecer outras vertentes, como: autocuidado, diagnóstico precoce e outros fatores associados ao diabetes mellitus tipo 2.
Local Viewer Preferences for Video Reaction Content About Indonesia by Creators on YouTube
Authors: Handoko Libri Annisa Firdayasa , Rafi'ah Kurnia Khafidatur
Published: October 2023

Indonesia has about 127 million active YouTube users, equivalent to 21,42% of the platform’s worldwide market share. This is certainly an ample opportunity for YouTube video creators to reach huge viewers of Indonesians. One of the content formats often used by creators to gain the attention of local audiences is the reaction content format. By reacting to viral content, it is easier to get exposure from viewers. This study aims to determine the local viewer preferences for reaction content influenced by the type of reacted content, video attributes of reaction content, self-impression of authentic videos that are given reactions, and the creator’s characteristics and behavior. To support the research findings, 109 active students of FEB UNPAD in 2022/2023 were involved as respondents. Based on the descriptive and inferential analysis results executed by the software IBM SPSS Statistics 29.0., each independent variable positively impacts the viewer's interest in watching reaction content, proved by both t and F statistic significances.
Analysis of Vehicle Operating Cost Efficiency in IECO Trans Company Using Transportation Methods
Authors: Soneda Ahmad Surya , Lestari Yetty Dwi
Published: October 2023

This research arose because of problems with travel costs, which the company judged were not optimal, and the profits obtained by the company were also not optimal. This research was taken from a case study at the IECO Trans company. This study aims to achieve the efficiency of the IECO Trans Company using the Transportation method. The method used from the transportation method is the northwest corner, the least cost combination, and the Vogel Approximation Method (VAM). This study uses qualitative research methods. Two data are used in this study: primary, taken from interviews and company data, and secondary, taken from previous research journals, websites, government data, and books. The results of this study will show the composition of route destinations and types of vehicles at the IECO Trans company, which results in cost efficiency. As an implementation of transportation methods and cost efficiency, the IECO Trans company will get options from the composition of route destinations and vehicle types based on analyzing transportation methods with a more efficient cost.