bluuebunny/crossref_metadata_2025 huggingface dataset:
Abstract 77: Pilot Outcomes Of A Multi-component Support Intervention For Caregivers Of Persons With Heart Failure
Authors: Abshire Saylor Martha , Pavlovic Noelle , DeGroot Lyndsay , Peeler Anna , Nelson Katie , Perrin Nancy , Gilotra Nisha A , Wolff Jennifer , Davidson Patricia , Szanton Sarah L
Published: May 2022

Background:
Caregivers of persons with heart failure must manage high levels of patient health care utilization, treatment complexity and often unpredictable stressors associated with intermittent symptom exacerbations and mortality. Interventions have often focused on the needs of the person with HF, not the caregiver. Therefore, we developed an intervention using human-centered design to provide caregiver-targeted support for this population.

Objective:
Pilot test the feasibility and gauge initial effect size of the
Caregiver Support
intervention to improve quality of life (mental and physical), caregiver burden, and self-efficacy among family caregivers from baseline to 16 weeks.

Methods:
The intervention includes five individualized, nurse-led sessions over 10 weeks conducted remotely (due to COVID-19). Intervention components focus on 1) nature of caregiving, 2) life purpose, 3) co-development of an action plan to address caregiver goals to reduce caregiver burden and improve caregiver well-being, 4) exploration of social and community resources to support unmet needs, and 5) building a sustainability plan for addressing future caregiver needs.

We tested our approach in a randomized waitlist control pilot trial (N=35) from August 2020 through March 2022. We calculated enrollment and retention rates, described acceptability, and computed intervention effect sizes from baseline to 16 weeks.

Results:
35 out of 101 (35%) eligible caregivers enrolled and were majority female (93.3%), White (60%) and spousal caregivers (63.3%). Average age was 59.4 ± 16.6 years. Overall retention was 69%. All intervention participants completed the five core components, reporting high levels of satisfaction and acceptability of activities. Between-group effect sizes (n=21) at 16 weeks suggest improvement in the mental health component of quality of life, caregiver burden, and self-efficacy (effect sizes 0.88, 0.31, and 0.63, respectively).

Conclusion:
Caregivers found
Caregiver Support
acceptable and study methods were feasible, despite challenges to engaging during the COVID-19 pandemic. Findings provide foundational evidence that this person-centered behavioral intervention can contribute to enhanced caregiver outcomes.
Abstract 80: Subtypes Of Fatigue In Heart Failure In The Atherosclerosis Risk In Communities Study
Authors: Pavlovic Noelle , Abshire Saylor Martha , Ndumele Chiadi E , Dennison Himmelfarb Cheryl R , Lee Christopher S , Szanton Sarah , Leoutsakos Jeannie-Marie
Published: May 2022

Background:
Heart failure (HF) affects ~6 million people in the US and is associated with marked morbidity and adverse clinical outcomes. Among HF patients, fatigue is common and distressing symptoms that significantly impacts patient quality of life and function. Current literature primarily examines fatigue as a single and generalized symptom. Fatigue is hypothesized, however, to manifest as multiple types, with both general and exertional components. Unique subtypes of fatigue in HF may require differential assessment and treatment to improve patient-reported and clinical outcomes.

Objective:
To identify unique fatigue subtypes in persons with prevalent HF in the Atherosclerosis Risk in Communities (ARIC) study.

Methods:
We performed a cross-sectional analysis of 1,114 participants at Visit 5 (2011-13) of the ARIC study with prevalent HF. We used the Modified Medical Research Council Breathlessness scale obtained at Visit 5 to assess exertional fatigue, and the PROMIS fatigue scale obtained at the 1
st
semi-annual follow up after Visit 5 (2012-2014) to assess general fatigue. We used latent class analysis to identify unique subtypes of fatigue based on patterns of responses to each of the scales.

Results:
Participants were 54% female and 38% Black with a mean age of 77 ± 5.5. We identified four latent classes of fatigue symptoms: 1) low/no fatigue, 2) high exertional & moderate general fatigue, 3) high general & low exertional fatigue, and 4) high general & exertional fatigue. The low/no fatigue class was the largest with an estimated 35.2% of the sample. The high exertional & moderate general class and the high general & low exertional class had similar estimated prevalences (23.5% and 23.8%, respectively). The high general & high exertional class was the smallest with an estimated 17.4% prevalence.

Conclusions:
We identified unique subtypes of fatigue in HF patients that have not been previously described in the literature. Future studies should examine the relationship between these latent classes of fatigue type and important patient-reported and clinical HF outcomes. Understanding these fatigue types and their relationships to outcomes may enhance our understanding of the symptom experience and inform prognostication in patients with HF.
Emerging Viral Infections and the Potential Impact on Hypertension, Cardiovascular Disease, and Kidney Disease
Authors: Savedchuk Solomiia , Raslan Rasha , Nystrom Sarah , Sparks Matthew A.
Published: May 2022

Viruses are ubiquitous in the environment and continue to have a profound impact on human health and disease. The COVID-19 pandemic has highlighted this with impressive morbidity and mortality affecting the world’s population. Importantly, the link between viruses and hypertension, cardiovascular disease, and kidney disease has resulted in a renewed focus and attention on this potential relationship. The virus responsible for COVID-19, SARS-CoV-2, has a direct link to one of the major enzymatic regulatory systems connected to blood pressure control and hypertension pathogenesis, the renin-angiotensin system. This is because the entry point for SARS-CoV-2 is the ACE2 (angiotensin-converting enzyme 2) protein. ACE2 is one of the main enzymes responsible for dampening the primary effector peptide Ang II (angiotensin II), metabolizing it to Ang-(1-7). A myriad of clinical questions has since emerged and are covered in this review. Several other viruses have been linked to hypertension, cardiovascular disease, and kidney health. Importantly, patients with high-risk apolipoprotein L1 (
APOL1
) alleles are at risk for developing the kidney lesion of collapsing glomerulopathy after viral infection. This review will highlight several emerging viruses and their potential unique tropisms for the kidney and cardiovascular system. We focus on SARS-CoV-2 as this body of literature in regards to cardiovascular disease has advanced significantly since the COVID-19 pandemic.
Thalictrum fusiforme (Ranunculaceae), described from southeastern Xizang (Tibet), China, is merged with T. rostellatum, a Sino-Himalayan species
Authors: ZENG YOU-PAI , YUAN QIONG , YANG QIN-ER
Published: May 2022

Based on critical observations on herbarium specimens (including type material) and living plants in the wild, we demonstrate that Thalictrum fusiforme, described from Zayu county in southeastern Xizang (Tibet), China, is conspecific with T. rostellatum, a Sino-Himalayan species widely distributed in Bhutan, China (southwestern and southeastern Xizang, northwestern Yunnan), northern India, and Nepal. We therefore reduce T. fusiforme to the synonymy of T. rostellatum herein. The geographical distribution of T. rostellatum in China is also clarified through the correction of some misidentifications, and lectotypification is proposed for T. rostellatum.
Observations on Pluteaceae in Vietnam. 3. One new species of Volvariella
Authors: MALYSHEVA EKATERINA , POPOV EUGENE
Published: May 2022

One new species of Volvariella (V. orientalis) is described based on materials collected from Vietnam. The species is characterized by large, stout basidiocarps with pilose-squamulose pileus, saccate volva, basidiospores 7.5–8.5 × 5.0–6.0 µm, and mucronate cheilocystidia. The macro- and microscopic descriptions with discussion on similar taxa are given, and their taxonomic position is supported by phylogenetic analyses based on nrITS and nrLSU regions.
Coastal Trapped Waves: Normal Modes, Evolution Equations, and Topographic Generation
Authors: Kelly Samuel M. , Ogbuka Sebastine
Published: May 2022

Coastal trapped waves (CTWs) transport energy along coastlines and drive coastal currents and upwelling. CTW modes are nonorthogonal when frequency is treated as the eigenvalue, preventing the separation of modal energy fluxes and quantification of longshore topographic scattering. Here, CTW modes are shown to be orthogonal with respect to energy flux (but not energy) when the longshore wavenumber is the eigenvalue. The modal evolution equation is a simple harmonic oscillator forced by longshore bathymetric variability, where downstream distance is treated like time. The energy equation includes an expression for modal topographic scattering. The eigenvalue problem is carefully discretized to produce numerically orthogonal modes, allowing CTW amplitudes, energy fluxes, and generation to be precisely quantified in numerical simulations. First, a spatially uniform K1 longshore velocity is applied to a continental slope with a Gaussian bump in the coastline. Mode-1 CTW generation increases quadratically with the amplitude of the bump and is maximum when the bump’s length of coastline matches the natural wavelength of the CTW mode, as predicted by theory. Next, a realistic K1 barotropic tide is applied to the Oregon coast. The forcing generates mode-1 and mode-2 CTWs with energy fluxes of 6 and 2 MW, respectively, which are much smaller than the 80 MW of M2 internal-tide generation in this region. CTWs also produce 1-cm sea surface displacements along the coast, potentially complicating the interpretation of future satellite altimetry. Prospects and challenges for quantifying the global geography of CTWs are discussed.
The Sensitivity of Downstream Ridge Building Forecasts to Upstream Warm Conveyor Belt Forecast Uncertainty Using MPAS
Authors: Berman Jeremy D. , Torn Ryan D.
Published: May 2022

One potential way to improve the skill of medium-range weather forecasts is to improve the evolution of Rossby waves, which largely modulate extratropical weather. Recent research has hypothesized that the predictability of downstream Rossby waves may be limited by forecast uncertainty linked to upstream diabatic processes such as latent heat release within the warm conveyor belt (WCB) of extratropical cyclones. This hypothesis is evaluated using Model for Prediction Across Scales (MPAS) ensemble forecasts for two events characterized by highly amplified flow over the North Atlantic associated with cyclogenesis. The source of variability in ridge forecasts is diagnosed using the ensemble-sensitivity technique and a potential vorticity (PV) tendency budget, which quantifies the contribution from individual physical processes toward subsequent ridge amplification. Before the onset of ridge amplitude differences for both events, ensemble forecasts with a more amplified ridge are associated with greater negative PV advection by the irrotational wind. The importance of PV advection by the irrotational wind suggests that PV changes are modulated by diabatic heating, which is confirmed by the sensitivity of ridge amplitude to earlier diabatic heating and lower-tropospheric moisture within an upstream WCB. After the onset of ridge amplitude differences, PV advection by the nondivergent wind becomes the primary driver of downstream forecast differences. Initial condition perturbations within the sensitive areas of the WCB confirm that increasing the initial lower-tropospheric moisture results in a more amplified ridge. This suggests that more accurate initial conditions near the WCB could lead to better downstream forecasts.
CEO Compensation and Cronyism in Emerging Economies: Evidence from India
Authors:   and, Jangra Abhishek , Gopalaswamy Arun Kumar
Published: May 2022

This study investigates the cronyism evidence in Indian listed firms and examines if excessive CEO and directors compensation reflect pay reciprocity impacting firm performance. Further the impact of CEO’s ties with the controlling owner on excess compensation, pay reciprocity and cronyism is also examined. OLS fixed effects regression on NSE 500 firms during 2002–2020 has been used for investigating the evidence of cronyism and the impact of CEOs’ ties with the controlling owner on cronyism. Based on the analysis it is observed that CEO compensation is strongly linked to CEO characteristics and corporate governance in addition to economic determinants. Consistent with reciprocity norms, we report a positive relationship between excess CEO compensation and excess director compensation, especially in firms where the CEO has ties to the controlling owner. However, pay reciprocity does benefit shareholders by improving subsequent firm value. Our findings indicate that excess CEO compensation increases subsequent firm value, especially in firms where the CEO has ties to the controlling owner.
Gurzhiite, Al(UO2)(SO4)2F⋅10H2O, a new uranyl sulfate mineral with a chain structure from the Bykogorskoe deposit, Northern Caucasus, Russia
Authors: Kasatkin Anatoly V. , Plášil Jakub , Chukanov Nikita V. , Škoda Radek , Nestola Fabrizio , Agakhanov Atali A. , Belakovskiy Dmitry I.
Published: May 2022

Gurzhiite, ideally Al(UO2)(SO4)2F⋅10H2O, is a new uranyl sulfate mineral from the Bykogorskoe U deposit, Northern Caucasus, Russia. It occurs as fine-grained aggregates forming veinlets up to 50 cm long in cracks of the brecciated rock. Gurzhiite aggregates are composed of small bladed crystals up to 0.1 mm across. Associated minerals include khademite and quartz. Gurzhiite is pale yellow in crystals, lemon yellow in aggregates, transparent with a vitreous lustre and a white streak. It is brittle and has an irregular fracture. Cleavage is good on {001}. The new mineral exhibits a bright yellow–green fluorescence under both longwave and shortwave UV radiation. Mohs hardness is ~2. Dmeas = 2.52(3) g/cm3 and Dcalc = 2.605 g/cm3. The mineral is biaxial (–) with α = 1.528(3), β = 1.538(2), γ = 1.544(3) (589 nm); 2Vmeas= 80(10)° and 2Vcalc = 75.1°. The empirical formula calculated on the basis of 21(O + F) atoms per formula unit (apfu) is Al0.92Zn0.05Fe3+0.03Na0.03U0.95S2.00O9.85F0.99⋅10.16H2O. Gurzhiite is triclinic, with space group P$\bar{1}$, a = 7.193(2), b = 11.760(2), c = 11.792(2) Å, α = 67.20(3), β = 107.76(3), γ = 89.99(3)°, V = 867.7(4) Å3 and Z = 2. The five strongest lines of the powder X-ray diffraction pattern [d, Å (I, %)(hkl)] are: 10.24(100)(001); 5.40(14)($\bar{ 1}\bar{1}$1); 5.11(54)(002); 3.405(11)($\bar{2}$11); and 3.065(11)($\bar{1}\bar{1}$3). The crystal structure of gurzhiite is based upon uranyl sulfate chains of the same type as in bobcookite and svornostite. Between the chains are two types of Al-octahedra – Al1(H2O)6 and Al2F2(H2O)4. The entire structure stability is maintained by a complex network of H bonds. The new mineral honours Russian mineralogist and crystallographer Dr. Vladislav V. Gurzhiy in recognition for his contributions to uranium mineralogy and crystallography.
Marker-free genome editing in the edible mushroom, Pleurotus ostreatus, using transient expression of genes required for CRISPR/Cas9 and for selection
Authors: Koshi Daishiro , Ueshima Hiroki , Kawauchi Moriyuki , Nakazawa Takehito , Sakamoto Masahiro , Hirata Mana , Izumitsu Kosuke , Sumita Takuya , Irie Toshikazu , Honda Yoichi
Published: May 2022

In a previous study, we reported a transient transformation system using repeated screening for hygromycin B (Hyg) resistance in the basidiomycete Ceriporiopsis subvermispora. In the present study, by combining this technique with CRISPR/Cas9, we demonstrated successful marker-free genome editing in Pleurotus ostreatus, which is one of the most economically important cultivated mushrooms as well as a model white-rot fungus. At first, transformant selection mediated by the transient expression of marker genes was demonstrated using a plasmid harboring the Hyg resistance gene (hph) in P. ostreatus. Then, genome editing of fcy1, which confers 5-fluorocytosine (5-FC) resistance to the host cell, was performed by the transient expression of Cas9, gRNA, and hph and strains with 5-FC resistance and Hyg sensitivity were isolated. Additionally, genome editing of fcy1 in these strains was confirmed by Sanger sequencing. To our knowledge, this is the first report of marker-free genome editing through the transient expression of Cas9, gRNA, and hph in agaricomycetes, which opens the door for repeated genome editing in these fungi.
New anti-tumor strategy based on acid-triggered self-destructive and near-infrared laser light responses of nano-biocatalysts integrating starvation–chemo–photothermal therapies
Authors: Liu Kanglin , Yan Shaoying , Liu Zhengshu , Wang Dan , Yang Qing , Jiang Xinyun , Chen Liuxian , Tang Hua
Published: May 2022

Background

Inherent limitations of single cancer therapy are overcome by multi-therapy modality, which integrates characteristics of each therapeutic modality and material chemistry. The multi-modal method has the potential for becoming one of the next generation options for cancer treatments. Photothermal therapy (PTT) is an efficient, non-invasive treatment method that can be used on various cancer types. We propose an acid-triggered self-destructing nano-biocatalyst integrated starvation/chemical/photothermal triple therapy that is based on design principles and biomedical applications of GOx cancer treatment methods.

Methods

Scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potentials were used to analyze the physical as well as chemical properties of MoS2@DOX/GOx@MnO2 (M@D/G@M). Further, Fourier transform infra-red (FTIR), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) were used to assess the compositions of the nanocatalysts. The biological effects of M@D/G@M on cells were studied in vitro by inverted fluorescence microscopy, confocal laser scanning microscopy (CLSM), flow cytometry, CCK-8 test, and hemolysis test. Treatment effects of the nanocatalysts were evaluated in MHCC-97H tumor BALB/c mice, whose body weights, tumor local temperature, tumor volumes, and tumor histological changes were evaluated.

Results

There was a high DOX encapsulation efficiency of M@D/G@M (90.233%). The photothermal conversion efficiency (η) of M@D/G@M is 25.2%, and its oxygen production within 5 min reached 27.5 mg L−1. Cell internalization analysis showed that within 4 h, M@D/G@M was almost completely absorbed by HepG2 cells. Further, the highest red fluorescence and apoptosis effects of dead cells (59.07% apoptosis) as well as the lowest tumor volume index of mice (0.2862%) were observed in the M@D/G@M + pH6.0 + NIR treatment group.

Conclusions

Our findings inform the development and applications of multi-modal methods in tumor therapy.
Characterization of qnrB-carrying plasmids from ESBL- and non-ESBL-producing Escherichia coli
Authors: Juraschek Katharina , Malekzadah Janina , Malorny Burkhard , Käsbohrer Annemarie , Schwarz Stefan , Meemken Diana , Hammerl Jens Andre
Published: May 2022

Background

Escherichia coli carrying clinically important antimicrobial resistances [i.e., against extended-spectrum-beta-lactamases (ESBL)] are of high concern for human health and are increasingly detected worldwide. Worryingly, they are often identified as multidrug-resistant (MDR) isolates, frequently including resistances against quinolones/fluoroquinolones.

Results

Here, the occurrence and genetic basis of the fluoroquinolone resistance enhancing determinant qnrB in ESBL-/non-ESBL-producing E. coli was investigated. Overall, 33 qnrB-carrying isolates out of the annual German antimicrobial resistance (AMR) monitoring on commensal E. coli (incl. ESBL-/AmpC-producing E. coli) recovered from food and livestock between 2013 and 2018 were analysed in detail. Whole-genome sequencing, bioinformatics analyses and transferability evaluation was conducted to characterise the prevailing qnrB-associated plasmids. Furthermore, predominant qnrB-carrying plasmid-types were subjected to in silico genome reconstruction analysis. In general, the qnrB-carrying E. coli were found to be highly heterogenic in their multilocus sequence types (STs) and their phenotypic resistance profiles. Most of them appeared to be MDR and exhibited resistances against up to ten antimicrobials of different classes. With respect to qnrB-carrying plasmids, we found qnrB19 located on small Col440I plasmids to be most widespread among ESBL-producing E. coli from German livestock and food. This Col440I plasmid-type was found to be highly conserved by exhibiting qnrB19, a pspF operon and different genes of unassigned function. Furthermore, we detected plasmids of the incompatibility groups IncN and IncH as carriers of qnrB. All qnrB-carrying plasmids also exhibited virulence factors and various insertion sequences (IS). The majority of the qnrB-carrying plasmids were determined to be self-transmissible, indicating their possible contribution to the spread of resistances against (fluoro)quinolones and other antimicrobials.

Conclusion

In this study, a diversity of different plasmid types carrying qnrB alone or in combination with other resistance determinants (i.e., beta-lactamase genes) were found. The spread of these plasmids, especially those carrying antimicrobial resistance genes against highest priority critically important antimicrobial agents, is highly unfavourable and can pose a threat for public health. Therefore, the dissemination pathways and evolution of these plasmids need to be further monitored.
Comparative transcriptome and metabolome analyses reveal the methanol dissimilation pathway of Pichia pastoris
Authors: Yu Yi-fan , Yang Jiashuo , Zhao Fengguang , Lin Ying , Han Shuangyan
Published: May 2022

Background

Pichia pastoris (Komagataella phaffii) is a model organism widely used for the recombinant expression of eukaryotic proteins, and it can metabolize methanol as its sole carbon and energy source. Methanol is oxidized to formaldehyde by alcohol oxidase (AOX). In the dissimilation pathway, formaldehyde is oxidized to CO2 by formaldehyde dehydrogenase (FLD), S-hydroxymethyl glutathione hydrolase (FGH) and formate dehydrogenase (FDH).

Results

The transcriptome and metabolome of P. pastoris were determined under methanol cultivation when its dissimilation pathway cut off. Firstly, Δfld and Δfgh were significantly different compared to the wild type (GS115), with a 60.98% and 23.66% reduction in biomass, respectively. The differential metabolites between GS115 and Δfld were mainly enriched in ABC transporters, amino acid biosynthesis, and protein digestion and absorption. Secondly, comparative transcriptome between knockout and wild type strains showed that oxidative phosphorylation, glycolysis and the TCA cycle were downregulated, while alcohol metabolism, proteasomes, autophagy and peroxisomes were upregulated. Interestingly, the down-regulation of the oxidative phosphorylation pathway was positively correlated with the gene order of dissimilation pathway knockdown. In addition, there were significant differences in amino acid metabolism and glutathione redox cycling that raised our concerns about formaldehyde sorption in cells.

Conclusions

This is the first time that integrity of dissimilation pathway analysis based on transcriptomics and metabolomics was carried out in Pichia pastoris. The blockage of dissimilation pathway significantly down-regulates the level of oxidative phosphorylation and weakens the methanol assimilation pathway to the point where deficiencies in energy supply and carbon fixation result in inefficient biomass accumulation and genetic replication. In addition, transcriptional upregulation of the proteasome and autophagy may be a stress response to resolve formaldehyde-induced DNA–protein crosslinking.
The knowledge, attitude and practice of community people on dengue fever in Central Nepal: a cross-sectional study
Authors: Phuyal Parbati , Kramer Isabelle Marie , Kuch Ulrich , Magdeburg Axel , Groneberg David A , Lamichhane Dhimal Mandira , Montag Doreen , Harapan Harapan , Wouters Edwin , Jha Anjani Kumar , Dhimal Meghnath , Müller Ruth
Published: May 2022

Background

Since 2006, Nepal has experienced frequent Dengue fever (DF) outbreaks. Up to now, there have been no knowledge, attitude and practice (KAP) studies carried out on DF in Nepal that have included qualitative in-depth and quantitative data. Thus, we aimed to explore and compare the KAP of people residing in the lowland (< 1500 m) and highland (> 1500 m) areas of Nepal.

Methods

A cross-sectional mixed-method study was conducted in six districts of central Nepal in September–October 2018 including both quantitative (660 household surveys) and qualitative data (12 focus group discussions and 27 in-depth interviews). The KAP assessment was executed using a scoring system and defined as high or low based on 80% cut-off point. Logistic regression was used to investigate the associated factors, in quantitative analysis. The deductive followed by inductive approach was adopted to identify the themes in the qualitative data.

Results

The study revealed that both the awareness about DF and prevention measures were low. Among the surveyed participants, 40.6% had previously heard about DF with a significantly higher number in the lowland areas. Similarly, IDI and FGD participants from the lowland areas were aware about DF, and it’s associated symptoms, hence they were adopting better preventive practices against DF. The findings of both the qualitative and quantitative data indicate that people residing in the lowland areas had better knowledge on DF compared to people in highland areas. All IDI participants perceived a higher chance of increasing future dengue outbreaks due to increasing temperature and the mobility of infected people from endemic to non-endemic areas. The most quoted sources of information were the television (71.8%) and radio (51.5%). Overall, only 2.3% of the HHS participants obtained high knowledge scores, 74.1% obtained high attitude scores and 21.2% obtained high preventive practice scores on DF. Among the socio-demographic variables, the area of residence, educational level, age, monthly income, SES and occupation were independent predictors of knowledge level, while the education level of the participants was an independent predictor of the attitude level.

Conclusions

Our study found a very low level of knowledge and insufficient preventive practices. This highlights an urgent need for extensive dengue prevention programs in both highland and lowland communities of Nepal.
Comparison of different treatment strategies in the management of endogenic caesarean scar pregnancy: a multicentre retrospective study
Authors: Qu Wenjie , Li Hua , Zhang Teng , Zhang Yuan , Ban Yanli , Li Ningfeng , Jiang Jingyan , Xie Juan , Shi Wentian , Hao Yiping , Li Ruowen , Liu Wei , Cui Baoxia
Published: May 2022

Background

The aim of this study was to evaluate the effectiveness and safety of different treatment strategies for endogenic caesarean scar pregnancy (CSP) patients.

Methods

According to Vial’s standard, we defined endogenic-type CSP as (1) the gestational sac growing towards the uterine cavity and (2) a greater than 0.3 cm thickness of myometrial tissue at the caesarean scar. A total of 447 endogenic CSP patients out of 527 patients from 4 medical centres in China were enrolled in this study. A total of 120 patients were treated with methotrexate (MTX) followed by surgery, 106 received ultrasound-guided curettage directly and 221 received curettage combined with hysteroscopy. The clinical information and clinical outcomes of these patients were reviewed. Successful treatment was defined as (1) no additional treatment needed, (2) no retained mass of conception and (3) serum β subunit of human chorionic gonadotropin (β-hCG) level returning to a normal level within 4 weeks. The success rate was analysed based on these factors.

Result

Among 447 patients, no significant difference was observed in baseline characteristics between groups except for foetal heartbeat. The success rate was significantly different (p<0.001) among the three groups. The highest success rate of 95.9% was noted in the hysteroscopy group, and the lowest success rate of 84.0% was noted in the curettage group. In addition, the MTX group reported the longest hospital stay and highest expenses, but the curettage group showed the shortest and lowest expenses, respectively. Nevertheless, no difference in blood loss was observed between the groups.

Conclusion

The combination of curettage and hysteroscopy represents the most effective strategy. Pretreatment with MTX did not result in better clinical outcomes. Ultrasound-guided curettage directly should not be considered a first-line treatment choice for endogenic CSP patients.
Factors that contribute to disparities in time to acute leukemia diagnosis in young people: an in depth qualitative interview study
Authors: Ding Lucky , Szymczak Julia E. , Evans Erica , Canepa Emma , Martin Ashley E. , Contractor Farah , Aplenc Richard , Joseph Galen , Winestone Lena E.
Published: May 2022

Background

Racial and ethnic disparities in outcomes for Black and Hispanic children with acute leukemia have been well documented, however little is known about the determinants of diagnostic delays in pediatric leukemia in the United States. The primary objective of this study is to identify factors contributing to delays preceding a pediatric leukemia diagnosis.

Methods

This qualitative study utilized in-depth semi-structured interviews. Parents and/or patients within two years of receiving a new acute leukemia diagnosis were asked to reflect upon their family’s experiences preceding the patient’s diagnosis. Subjects were purposively sampled for maximum variation in race, ethnicity, income, and language. Interviews were analyzed using inductive theory-building and the constant comparative method to understand the process of diagnosis. Chart review was conducted to complement qualitative data.

Results

Thirty-two interviews were conducted with a diverse population of English and Spanish speaking participants from two tertiary care pediatric cancer centers. Parents reported feeling frustrated when their intuition conflicted with providers’ management decisions. Many felt laboratory testing was not performed soon enough. Additional contributors to delays included misattribution of vague symptoms to more common diagnoses, difficulties in obtaining appointments, and financial disincentives to seek urgent or emergent care. Reports of difficulty obtaining timely appointments and financial concerns were disproportionately raised among low-income Black and Hispanic participants. Comparatively, parents with prior healthcare experiences felt better able to navigate the system and advocate for additional testing at symptom onset.

Conclusions

While there are disease-related factors contributing to delays in diagnosis, it is important to recognize there are multiple non-disease-related factors that also contribute to delays. Evidence-based approaches to reduce outcome disparities in pediatric cancer likely need to start in the primary care setting where timeliness of diagnosis can be addressed.
Mutation analysis reveals novel and known mutations in SAG gene in first two Egyptian families with Oguchi disease
Authors: Tawfik Caroline Atef , Elbagoury Nagham Maher , Khater Noha Ibrahim , Essawi Mona Lotfi
Published: May 2022

Background

Oguchi disease is a rare type of congenital stationary night blindness associated with an abnormal fundus appearance. It is inherited in an autosomal recessive manner where two types exist according to the gene affected; type 1 associated with S-antigen (SAG) gene mutations and type 2 associated with rhodopsin kinase (GRK1) gene mutations.

Purpose

The aim of this work was to describe the clinical and genetic findings of the first two reported families of Oguchi disease in Egypt and African region.

Methods

Four members of two consanguineous Egyptian families with history of night blindness since childhood underwent complete ophthalmological examination, standard automated static perimetry, fundus color photography, fundus autofluorescence (FAF), fundus fluorescein angiography (FFA) in light-adapted state and spectral-domain optical coherence tomography (SD-OCT) of both the macula and the optic nerve head as well as central corneal thickness with repeated fundus photography following prolonged dark adaptation. Mutation screening of 7 coding exons of GRK1 gene and 15 coding exons of SAG gene as well as some flanking regions were performed using Sanger sequencing technique. The variants were tested for pathogenicity using different in silico functional analysis tools.

Results

The clinical examination and investigations confirmed Oguchi disease phenotype. One patient showed p.R193* (c.577C > T) which is a previously reported SAG gene mutation in a homozygous form. The other three patients from a different family showed (c.649–1 G > C), a novel canonical splice site SAG gene mutation in a homozygous form.

Conclusion

The identification of the novel canonical splice site SAG gene variant in three members of the same family with clinically confirmed Oguchi disease reinforces its pathogenicity. A fourth patient from another family carried a previously reported mutation in the same gene. SAG gene variants may be the underlying genetic cause for Oguchi disease in Egypt. Our findings have expanded the spectrum of Oguchi disease-associated mutations in SAG gene and may serve as a basis for genetic diagnosis for Oguchi disease.
The association between dietary intake and cardiometabolic risk factors among obese adolescents in Indonesia
Authors: Murni Indah K. , Sulistyoningrum Dian C. , Susilowati Rina , Julia Madarina , Dickinson Kacie M.
Published: May 2022

Background and objective

Poor diets, characterized by excess fat, sugar and sodium intakes, are considered to be one of the most important modifiable risk factors for cardiovascular disease. Diet patterns and intakes during adolescence may persist into adulthood and impact on risk for chronic disease later in life. We aimed to evaluate the dietary intake of obese adolescents and its relationship to cardiometabolic health including lipid status and glycemic control.

Methods and study design

This was a cross-sectional study of obese children aged 15 to < 18 years in Yogyakarta, Indonesia. All children had a medical history performed including a physical examination and fasting blood sample. Dietary intake was assessed using a semi-quantitative recall food frequency questionnaire. Multivariable linear regression model was performed to determine the relationship between dietary intakes and cardiovascular disease risks and to adjust for potential confounders.

Results

Of 179 adolescents, 101 (57.4%) were male and median age was 16.4 (15.0–17.9) years. The majority of adolescents (98%) had inadequate intake of fibre and exceeded intakes of total fat (65%) and total sugar (36%). There was statistically significant correlation found in the multivariable linear regression analysis between fibre intake and HDL cholesterol after adjusting for potential confounders (β = 0.165; p = 0.033).

Conclusions

This study demonstrates that there is a high proportion of obese Indonesian adolescents with poor dietary intakes. There was relationship observed between intake of nutrients of concern (fibre) and cardiometabolic risk factor among this sample of obese adolescents. Future research should examine overall dietary patterns in more detail among this population to elucidate the role of poor diet intakes in development of cardiovascular disease risk factors in young people transitioning into adulthood.
Danger appraisal and pathogen-avoidance mechanisms in stigma towards severe mental illness: the mediating role of affective responses
Authors: Chamorro Coneo Ana , Aristizabal Diazgranados Edith , Hoyos de los Rios Olga , Aguilar Santander Daniela
Published: May 2022

Background

Stereotypes of dangerousness are common predictors of stigmatising attitudes towards Severe Mental Illness (SMI). However less is known about pathogen avoidance mechanisms underlying stigma towards SMI, specially in samples of non-industrialised societies of Latin America and the Caribbean. The primary aim of this study was to examine pathogen-disgust sensitivity and danger appraisal mechanisms in responses of stigma towards SMI.

Methods

Cross-sectional design with convenience sampling. Using an online survey, volunteers at the Universidad del Norte in Colombia (N = 271) provided their sociodemographic data and completed the Three-Domain Disgust Scale (TDDS). Participants were randomised to different descriptions of someone with SMI that varied in terms of aggressiveness (with and without danger) and causes of the SMI. Then, following the attribution questionnaire (AQ-27), respondents reported affective and discriminatory responses to the person in the description.

Results

Increased disgust sensitivity to pathogen stimuli resulted in stronger reports of anger (β = .14; p = .03), and fear (β = 0.27; p < 0.001). The relationship between disgust sensitivity and discriminatory responses was indirectly mediated by fear towards SMI (Bootstrapped CI =—.04,—.009). Dangerousness attributions in the description of SMI predicted stronger feelings of anger (β = .23; p = 0.001) and fear (β = .40; p < .001), as well increased support for coercion-segregation of SMI (β = .34; p = 0.04), but less intentions to help (β = -.26; p = 0.003). The relationship between dangerousness and support for coercion was mediated by fear (Bootstrapped CI = .72, 1.37) and anger (Bootstrapped CI = .06, .44), whereas pity (Bootstrapped CI = .03, .38) and fear (Bootstrapped CI = -1.39, -.69) mediated responses of support for coercion-segregation of SMI. Attributions about causes and personal responsibility were not significantly linked to stigma towards SMI (p > 0.05).

Conclusions

Findings suggested that pathogen avoidance and danger appraisal systems interplay in the generation of discriminatory behaviour towards SMI. Anti-stigma programs and policy makers would benefit from introducing strategies that challenge stereotypes of dangerousness and unpredictability by promoting positive contact with people with SMI.
Experiences of risk in Australian hotel quarantine: a qualitative study
Authors: Haire Bridget , Gilbert Gwendolyn L. , Kaldor John M. , Hendrickx David , Dawson Angus , Williams Jane H.
Published: May 2022

Background

In response to the threat of COVID-19 infection, Australia mandated a 14 day quarantine period in a designated facility for all travellers returning from overseas from late March 2020. These facilities were usually hotels, or hotel-like serviced apartments, and also included a repurposed former mining village in the Northern Territory. This paper aimed to investigate the experiences of risk of people quarantined in designated supervised facilities in Australia, which has not been systematically explored before.

Methods

In this qualitative study semi-structured interviews were conducted with 58 participants quarantined between March 2020 and January 2021. Participants were returned Australian citizens and residents who were required to undergo mandatory supervised quarantine for COVID-19. Interviews were conducted using video teleconferencing (via Zoom), transcribed and coded, then analysed thematically.

Results

While participants generally supported the concept of quarantine to protect the Australian public, they were critical of elements of it where they felt exposed to risk (COVID-related or not). They also described instances where infection control within the system seemed inadequate. For some, particularly those quarantined with small children, they reported that the facilities were inadequate or inappropriate for health and wellbeing. Using thematic analysis, three major themes were identified that related to problems in the existing system: perception of being subjected to high risk through lax standards of COVID protection in the quarantine process; risks to the community identified in quarantine; and risk in non-hotel managed quarantine facilities.

Conclusions

There are systemic issues with infection control in hotel quarantine, which can be further undermined by individual non-compliance. Risks to safety for those in quarantine can be reduced, both in terms of infection control within hotel quarantine and, in the case of the Northern Territory facility, timely in-person medical care as needed for non-COVID conditions. Systems of infection control need ongoing review to ensure that people entering quarantine are protected from known risks of infection at every stage. Medical services in quarantine facilities should be examined to ensure timely and appropriate non-COVID medical services are available.
Development and psychometric assessment an instrument for investigating Women’s attitude toward home safety
Authors: Ahmadpour-geshlagi Rasoul , Akbarinia Golam Reza , Gillani Neda , Karimkhani Fatemeh , Alizadeh Seyed Shamseddin , Nazari Jalil
Published: May 2022

Background

Approximately half of the Iranian population are women, and they play a vital role in the home. The women’s attitude can play a critical role in the safety of homes. Best of our knowledge, there is not a valid and reliable instrument to measure their attitude toward home safety. So, the present study aimed to design a psychometrics tool to assess women’s attitudes toward home safety.

Methods

The researchers designed an instrument based on the home safety concept as the first instrument to measure housewives’ attitudes toward home safety. The developed instrument distributed among 686 women in Tabriz health centers. Content validity, confirmatory, and exploratory factor analysis were used to examine the construct validity, and Cronbach’s alpha and test-retest were employed to examine the reliability and reproducibility of the instrument.

Results

In the face validity section, the impact score of all items was determined to be above 1.5. In the content validity section, 4 items were excluded from the 39 questionnaire items due to low Content Validity Ratio (CVR). The mean CVR of all items was 0.842. By conducting exploratory factor analysis, it was found that the questionnaire has six dimensions. Three questions were removed from the study due to lack of connection with other items. Also, Cronbach’s alpha coefficient of the questionnaire is equal to 0.924, which indicates the appropriate reliability of the instrument.

Conclusions

This study aimed to develop a questionnaire to assess the safety attitudes of housewives toward home safety. It was found that the prepared tool has acceptable validity and reliability.
System-level investigation of anti-obesity effects and the potential pathways of Cordyceps militaris in ovariectomized rats
Authors: Jang Dongyeop , Lee Eunjoo , Lee Sullim , Kwon Yongsam , Kang Ki Sung , Kim Chang-Eop , Kim Daeyoung
Published: May 2022

Background

Cordycepsspecies have been used as tonics to enhance energy, stamina, and libido in traditional Asian medicine for more than 1600 years, indicating their potential for improving reproductive hormone disorders and energy metabolic diseases. AmongCordyceps,Cordyceps militarishas been reported to prevent metabolic syndromes including obesity and benefit the reproductive hormone system, suggesting thatCordyceps militariscan also regulate obesity induced by the menopause. We investigated the effectiveness ofCordyceps militarisextraction (CME) on menopausal obesity and its mechanisms.

Methods

We applied an approach combining in vivo, in vitro, and in silico methods. Ovariectomized rats were administrated CME, and their body weight, area of adipocytes, liver and uterus weight, and lipid levels were measured. Next, after the exposure of MCF-7 human breast cancer cells to CME, cell proliferation and the phosphorylation of estrogen receptor and mitogen-activated protein kinases (MAPK) were measured. Finally, network pharmacological methods were applied to predict the anti-obesity mechanisms of CME.

Results

CME prevented overweight, fat accumulation, liver hypertrophy, and lowered triglyceride levels, some of which were improved in a dose-dependent manner. In MCF-7 cell lines, CME showed not only estrogen receptor agonistic activity through an increase in cell proliferation and the phosphorylation of estrogen receptors, but also phosphorylation of extracellular-signal-regulated kinase and p38. In the network pharmacological analysis, bioactive compounds of CME such as cordycepin, adenine, and guanosine were predicted to interact with non-overlapping genes. The targeted genes were related to the insulin signaling pathway, insulin resistance, the MARK signaling pathway, the PI3K–Akt signaling pathway, and the estrogen signaling pathway.

Conclusions

These results suggest that CME has anti-obesity effects in menopause and estrogenic agonistic activity. Compounds in CME have the potential to regulate obesity-related and menopause-related pathways. This study will contribute to developing the understanding of anti-obesity effects and mechanisms ofCordyceps militaris.
The effects of internet and social media use on the work performance of physicians and nurses at workplaces in Palestine
Authors: Ahmead Muna , Hamamadeh Nida , Iram Issa abu
Published: May 2022

Background

The use of internet and social media applications in the workplace has increased dramatically with both beneficial and harmful effects. Their use also appears to affect job performance in the workplace.

Aim

To assess the beneficial and harmful effects of internet and social media application use in the workplace, and on the work performance of health professional in the major 4 referral hospitals in the cities of Hebron and Bethlehem.

Method

A cross-sectional study was conducted using self-reported questionnaires: a work-related social media questionnaire (WSMQ), and the William Anderson Work Performance Scale. The sample of the study included 409 participants (169 physicians and 240 nurses).

Results

The Pearson correlation test showed a weak positive statistically significant relationship between the WSMQ and William Anderson Work Performance Scale. The findings revealed that the overall mean score for the WSMQ was high (mean score 3.57), and the higher mean was for the beneficial WSMQ compared with the harmful WSMQ. The mean for the William Anderson Work Performance Scale was moderate performance (3.35). The higher mean was for Organizational Citizenship Behavior-Individual (OCBI), followed by In Role Behavior (IRB).

Conclusion

Palestinian healthcare professionals should be encouraged to use internet and social media applications to improve health outcomes, and provide health information to the community rather than simply using these tools for communication. Administrative staff and policy makers in Palestinian hospitals should regulate the use of internet and social media applications in hospitals by developing a clear policy on this topic for the workplace.