bluuebunny/crossref_metadata_2025 huggingface dataset:
Pupils’ didactical Mileu in an inclusive discourse in basic triangle geometry
Authors: Ali Clement Ayarebilla , Asemani Emmanuel , Tangkur Michael
Published: September 2024

The concept of inclusivity is the practice of placing pupils with disabilities in normal classrooms. However, the idea of the triangle continues to pose fundamental challenges. This study sought to explore didactical mediation on the learning of the triangle beyond the classroom. The research design used mixed methods sequential exploratory to help identify the pupil’s interpretation of the triangle. The researchers first conducted a semi-structured interview on their experiences in learning the triangle both in school and at home. The responses were transcribed and coded in themes. The themes that emerged were properties, types of angles, and applications to everyday life. The researchers then went further to test the pupils’ knowledge on the same themes. The findings showed that didactical mediation with the cultural artefacts, signs, and language largely explained the emergence of the four main properties and must be targeted for the theory, policy, and practice of inclusive education. The prototype testing also confirmed that even though pupils with hearing impairment obtained higher scores, both groups obtained higher mean scores on the post-test than did the pre-test. This indicated that the learning achievement was significantly higher in the post-test than in the pre-test due to the didactical mediation. It was recommended that the triangle’s learning and teaching should not be limited to academic exercises but to real-life situations. Also, mathematics concepts that are not visually friendly be explored to uncover the most suitable methods and techniques to make inclusive mathematics learning a reality for all groups of disabilities.
Implementation of a hybrid mathematics module to minimize students’ learning obstacles when interpreting fractions
Authors: Isnawan Muhamad Galang , Belbase Shashidhar , Yanuarto Wanda Nugroho
Published: September 2024

During panic-gogy, learning problems increased, including problems in learning mathematics. Researchers considered parents as participants and used these parents’ considerations as a basis for preparing learning designs. The study aimed to look for alternative solutions to minimize obstacles to students’ mathematics learning during panic-gogy. The research design of this study was didactical design research. The participants were 56 students (13–18 years) and 71 parents (35–50 years) of students at one junior high school in Indonesia. The researcher used several instruments, such as a fraction meaning test, a semi-structured interview, a hybrid mathematical module, and a documentation study. NVivo-12 assisted in the thematic qualitative analysis of the data. The results showed that students had experienced obstacles in interpreting fractions because of the teacher’s limited understanding of fractions. Parents preferred face-to-face learning through online learning platforms. The hybrid mathematics module facilitated various meanings of fractions by integrating problems as situations in constructing these meanings and minimizing students’ learning barriers during panic-gogy.
Extracellular vesicles for cancer therapy: potential, progress, and clinical challenges
Authors: Ren Lili , Zhang Dingmei , Pang Long , Liu Shiyu
Published: September 2024

Extracellular vesicles (EVs) play an important role in normal life activities and disease treatment. In recent years, there have been abundant relevant studies focusing on EVs for cancer therapy and showing good performance on tumor inhibition. To enhance the effectiveness of EVs, EV analogs have been developed. This review summarizes the classification, origin, production, purification, modification, drug loading and cancer treatment applications of EVs and their analogs. Also, the characteristics of technologies involved are analyzed, which provides the basis for the development and application of biogenic vesicle-based drug delivery platform for cancer therapy. Meanwhile, challenges in translating these vesicles into clinic, such as limited sources, lack of production standards, and insufficient targeting and effectiveness are discussed. With ongoing exploration and clinical studies, EV-based drugs will make great contributions to cancer therapy.
The C57BL/6N mouse substrain is a viable model of elastase-induced abdominal aortic aneurysm
Authors: Wei Panpan , Li Kexin , Li Meng , Liu Haole , Xia Congcong , Chen Yajie , Zhao Sihai , Li Yankui
Published: September 2024

Aim

Compared with the C57BL/6N substrain, the C57BL/6J substrain is more susceptible to the angiotensin II (Ang II)-induced development of dissected abdominal aortic aneurysms (AAAs). The aim of this study was to elucidate whether the widely used C57BL/6N mouse substrain is as susceptible as the C57BL/6J mouse substrain to porcine pancreatic elastase (PPE) infusion-induced experimental nondissected AAA development.

Methods

Experimental nondissected AAAs were induced in C57BL/6J and C57BL/6N mice via transient aortic luminal infusion of PPE. On Day 0 (baseline) and Day 14 after PPE infusion, the abdominal aortic diameter was directly measured. Aortic aneurysmal segment samples were collected, and histopathological analysis was performed.

Results

On Day 14 after PPE infusion, aortic diameters were significantly increased in both mouse substrains (from approximately 0.51 to 1.24 mm in C57BL/6J mice and from 0.51 to 1.18 mm in C57BL/6N mice). The increase in diameter of all the mice exceeded 50% and met the criteria for AAA model establishment (143% and 135% in C57BL/6J mice and C57BL/6N mice, respectively). PPE infusion also induced obvious local aortic wall macrophage and T-cell infiltration, elastin degradation, smooth muscle cell depletion and high metallopeptidase (MMP)-2 and MMP-9 expression levels in C57BL/6N mice, but these differences were not significant compared with those in C57BL/6J mice. However, PPE infusion led to the recruitment of more B cells and the sprouting of more neovessels at the aneurysmal lesion site in C57BL/6J mice than in C57BL/6N mice.

Conclusion

The C57BL/6N mouse substrain is suitable for establishing a model of AAA via elastase infusion.
The influence of post-processing software on quantitative results in 4D flow cardiovascular magnetic resonance examinations
Authors: Trauzeddel Ralf F. , Müller Maximilian , Demir Aylin , Wiesemann Stephanie , Daud Elias , Schmitter Sebastian , Viezzer Darian , Hadler Thomas , Schulz-Menger Jeanette
Published: September 2024

Background

Several commercially available software packages exist for the analysis of three-dimensional cine phase-contrast cardiovascular magnetic resonance (CMR) with three-directional velocity encoding (four-dimensional (4D) flow CMR). Only sparse data are available on the impact of these different software solutions on quantitative results. We compared two different commercially available and widely used software packages and their impact on the forward flow volume (FFV), peak velocity (PV), and maximum wall shear stress (WSS) per plane.

Materials and methods

4D flow CMR datasets acquired by 3 Tesla magnetic resonance imaging of 10 healthy volunteers, 13 aortic stenosis patients, and 7 aortic valve replacement patients were retrospectively analyzed for FFV, PV, and WSS using two software packages in six analysis planes along the thoracic aorta. Absolute (AD) and relative differences (RD), intraclass correlation coefficients (ICC), Bland–Altman analysis, and Spearman's correlation analysis were calculated.

Results

For the FFV and PV in healthy volunteers, there was good to excellent agreement between both software packages [FFV: ICC = 0.93–0.97, AD: 0.1 ± 5.4 ml (−2.3 ± 2.4 ml), RD: −0.3 ± 8% (−5.7 ± 6.0%); PV: ICC = 0.81–0.99, AD: −0.02 ± 0.02 ml (−0.1 ± 0.1 ml), RD: −1.6 ± 2.1% (−9.3 ± 6.1%)]. In patients, the FFV showed good to excellent agreement [ICC: 0.75–0.91, AD: −1.8 ± 6.5 ml (−8.3 ± 9.9 ml), RD: −2.2 ± 9.2% (−13.8 ± 17.4%)]. In the ascending aorta, PV showed only poor to moderate agreement in patients (plane 2 ICC: 0.33, plane 3 ICC: 0.72), whereas the rest of the thoracic aorta revealed good to excellent agreement [ICC: 0.95–0.98, AD: −0.03 ± 0.07 (−0.1 ± 0.1 m/s), RD: −3.5 ± 7.9% (−7.8 ± 9.9%)]. WSS analysis showed no to poor agreement between both software packages. Global correlation analyses revealed good to very good correlation between FFV and PV and only poor correlation for WSS.

Conclusions

There was good to very good agreement for the FFV and PV except for the ascending aorta in patients when comparing PV and no agreement for WSS. Standardization is therefore necessary.
Automated pipeline for denoising, missing data processing, and feature extraction for signals acquired via wearable devices in multiple sclerosis and amyotrophic lateral sclerosis applications
Authors: Cossu Luca , Cappon Giacomo , Facchinetti Andrea
Published: September 2024

Introduction

The incorporation of health-related sensors in wearable devices has increased their use as essential monitoring tools for a wide range of clinical applications. However, the signals obtained from these devices often present challenges such as artifacts, spikes, high-frequency noise, and data gaps, which impede their direct exploitation. Additionally, clinically relevant features are not always readily available. This problem is particularly critical within the H2020 BRAINTEASER project, funded by the European Community, which aims at developing models for the progression of Multiple Sclerosis (MS) and Amyotrophic Lateral Sclerosis (ALS) using data from wearable devices.

Methods

The objective of this study is to present the automated pipeline developed to process signals and extract features from the Garmin Vivoactive 4 smartwatch, which has been chosen as the primary wearable device in the BRAINTEASER project. The proposed pipeline includes a signal processing step, which applies retiming, gap-filling, and denoising algorithms to enhance the quality of the data. The feature extraction step, on the other hand, utilizes clinical partners' knowledge and feedback to select the most relevant variables for analysis.

Results

The performance and effectiveness of the proposed automated pipeline have been evaluated through pivotal beta testing sessions, which demonstrated the ability of the pipeline to improve the data quality and extract features from the data. Further clinical validation of the extracted features will be performed in the upcoming steps of the BRAINTEASER project.

Discussion

Developed in Python, this pipeline can be used by researchers for automated signal processing and feature extraction from wearable devices. It can also be easily adapted or modified to suit the specific requirements of different scenarios.
A physics-based ensemble machine-learning approach to identifying a relationship between lightning indices and binary lightning hazard
Authors: Thomas Andrew M. , Noble Stephen
Published: September 2024

To convert lightning indices generated by numerical weather prediction experiments into binary lightning hazard, a machine-learning tool was developed. This tool, consisting of parallel multilayer perceptron classifiers, was trained on an ensemble of planetary boundary layer schemes and microphysics parameterizations that generated four different lightning indices over 1 week. In a subsequent week, the multi-physics ensemble was applied and the machine-learning tool was used to evaluate the accuracy. Unintuitively, the machine-learning tool performed better on the testing dataset than the training dataset. Much of the error may be attributed to mischaracterizing the convection. The combination of the machine learning model and simulations could not differentiate between cloud-to-cloud lightning and cloud-to-ground lightning, despite being trained on cloud-to-ground lightning. It was found that the simulation most representative of the local operational model was the most accurate simulation tested.
To each their own: sociodemographic disparities in student mental health
Authors: De Groot Kristel , Wieman Sander M. , Van Strien Jan W. , Lindemann Oliver
Published: September 2024

Research has demonstrated high rates of mental health problems in university students, and even higher rates in students belonging to historically marginalised populations. However, research on disparities in student mental health has almost exclusively focused on internalising problems (like symptoms of depression and anxiety), overlooking other ways in which individuals experience and express negative emotion. To address this limitation, the present study employed the Brief Problem Monitor to examine three types of mental health problems—internalising, externalising, and attentional problems—across six sociodemographic characteristics: gender (male vs. female), sexual orientation (heterosexual vs. non-heterosexual), ethnicity (Dutch without vs. with migration background), internationality (domestic vs. international), disability (no disabilities vs. disabilities), and parental education (continuing- vs. first-generation). These sociodemographic variables were included in regression models simultaneously, thereby controlling for the effects of the others. Across a sample of 2,256 students, internalising problems were significantly higher in students who were female, non-heterosexual, international, and in those with disabilities. Higher externalising problems were found in female students, students with disabilities, those with a migration background, and first-generation students. Finally, attentional problems were higher in non-heterosexual students and students with disabilities. These findings support the premise that different groups of students experience different types of mental health concerns, emphasising the importance of measuring mental health multidimensionally. As an example, had the present study only examined internalising problems, we would not have found mental health disparities for students with a migration background and first-generation students, which has clear implications for outreach and resources offered to them.
Promoting student engagement: insights from Iceland, Lithuania, and Norway
Authors: Petronienė Saulė , Juzelėnienė Saulutė , Tautkevičienė Gintarė , Eyjólfsdóttir Emma Björg , Stebbins Randi Whitney , Dovidonytė Rasa
Published: September 2024

Introduction

The complex phenomenon of engagement in the research draws upon the sociocultural theories and emphasizes the three interrelated dimensions: behavioral, emotional/affective, and cognitive. Student engagement in academic life is a key factor for student wellbeing, making it essential to address the issues related to student engagement to be able to foster overall academic success. The research has shown that the COVID-19 pandemic impacted the academic environment including students' performance and their social and private lives.

Methodology

In order to help young people prepare to become students, an analysis of student needs and experiences was carried out at three universities: the University of Stavanger (Norway), Kaunas University of Technology (Lithuania), and the University of Iceland (Iceland). Focus group discussions were used to collect data about the students' experience entering university and what challenges first-year students faced before and during the COVID-19 pandemic. A total of 17 first-year (6 males and 11 females) who started their studies during COVID-19 lockdown and 16 second-year students (9 males and 7 females) who started theirs studies before COVID-19 participated in the focus groups discussions. The collected data consists of students' responses during the focus group discussions. The qualitative thematic content analysis was used to identify emergent themes and categories.

Results

The results of the research indicate that students face several challenges when entering university: adapting to the academic environment and the student role, information overload, social relationships, communication, and self-esteem issues. Students who started their studies during the pandemic indicated different experiences and challenges in adapting to university life.

Discussion

This research faced several constraints, including the limited sample size, the focus on only three universities in Northern Europe, and the reliance on self-reported data from the focus group interviews, which may not fully capture the diversity of student experiences. Despite these limitations, the research provides valuable insights into the impact of the COVID-19 pandemic on student engagement and the unique challenges faced by new students during this period. The results of this research confirm the findings of previous research claiming that the impacts of the COVID-19 pandemic vary depending on the environment. Students need a variety of targeted and /or intensive support services to address their increased social, emotional, and academic needs.
Challenges in molecular diagnosis of multiple endocrine neoplasia
Authors: Romanet Pauline , Charnay Théo , Sahakian Nicolas , Cuny Thomas , Castinetti Frédéric , Barlier Anne
Published: September 2024

Multiple endocrine neoplasia (MEN) is a group of rare genetic diseases characterized by the occurrence of multiple tumors of the endocrine system in the same patient. The first MEN described was MEN1, followed by MEN2A, and MEN2B. The identification of the genes responsible for these syndromes led to the introduction of family genetic screening programs. More than twenty years later, not all cases of MENs have been resolved from a genetic point of view, and new clinicogenetic entities have been described. In this review, we will discuss the strategies and difficulties of genetic screening for classic and newly described MENs in a clinical setting, from limitations in sequencing, to problems in classifying variants, to the identification of new candidate genes. In the era of genomic medicine, characterization of new candidate genes and their specific tumor risk is essential for inclusion of patients in personalized medicine programs as well as to permit accurate genetic counseling to be proposed for families.
Modeling potential benefits of fragmented marsh terrace restoration in Terrebonne bay, Louisiana: sediment processes interacting with vegetation and potential submerged aquatic vegetation habitat
Authors: Jung Hoonshin , Moss Leland , Carruthers Tim J. B. , Di Leonardo Diana R. , DeMarco Kristin , Whalen Marie , Brasher Michael , Dijkstra Jasper
Published: September 2024

Marsh terraces, constructed as a restoration and protection strategy, consist of a series of earthen berms in open water areas of the coastal wetland landscape and are being implemented across the Louisiana coast. To assess the efficacy of the marsh terraces as a nature-based solution, a small-scale, high-resolution hydrodynamic model was developed based on field sampling of vegetation and physical parameters (water level, waves, sediment, turbidity, and terrace elevation). This study tested common marsh terrace designs (e.g., chevron, linear, box, T-shape, etc.), ultimately selecting a preferred design based on the evaluation of factors such as vegetation, water depth, and sediment type on terrace stability and sediment retention under calm and storm conditions. The model results revealed that the 100 m box and the chevron designs exhibited greatest terrace stability and sediment trapping, particularly when installed perpendicular to prevailing wind and waves. The preferred terrace design was the box design due to its higher modeled resilience to wind and waves from multiple directions. Vegetation presence enhanced terrace resistance to erosion, with variations depending on vegetation type. Higher vegetation biomass, especially during the summer, contributed to the greatest stability of terraces. Greater water depth between terraces led to increased sediment retention, and terraces predominantly composed of organic-rich mud demonstrated greater stability than those with higher proportions of sand. Overall, vegetation had the greatest impact on sediment retention in the terrace field compared to water depth and sediment type. However, the potential habitat for submerged aquatic vegetation (SAV) was more influenced by water depth (i.e., 0.1 m < depth <1 m) than shear stress (<0.5 Pa). Even under storm conditions, shear stress rarely determined potential habitat for SAV, as shear stress remained relatively low within the terrace field. Potential SAV habitat was most abundant in shallow areas and increased where sediment stability was lowest (i.e., no vegetation and sand), primarily due to eroded sediment increasing the shallow area. While this model was developed using field data specific to Louisiana marshes, it can be adapted as a tool for terrace restoration project design and planning in most coastal wetlands.
An evaluation of fin ray microchemistry to describe movement of White Sturgeon in the Kootenai River basin: insights and limitations
Authors: Ghere Courtnie L. , Quist Michael C. , Hardy Ryan S. , Willmes Malte , Lewis Levi S. , Wilson Sean M. , Smith Troy W.
Published: September 2024

Introduction

White Sturgeon Acipenser transmontanus in the Kootenai River basin is listed as endangered in the United States and Canada. Declines have been mainly attributed to poor recruitment exacerbated by the environmental effects of Libby Dam in Montana. Reduced primary production downstream of Libby Dam has been identified as one factor limiting growth of White Sturgeon, thereby limiting natural reproduction through delayed sexual maturity. However, estimating changes in growth over time without knowledge of fish location (i.e., Kootenai River vs. Kootenay Lake) is difficult. The objective of this project was to use microchemistry analysis to describe the movement of White Sturgeon within the Kootenai River basin.

Methods

Water samples (n = 27) and White Sturgeon fin rays (n = 162) collected in the Kootenai River basin were measured for strontium isotope (87Sr:86Sr) ratios using laser ablation multicollector inductively coupled plasma mass spectrometry. All water samples and a subset of fin rays (n = 8) were also analyzed for trace elements (e.g., Sr, Ba, Mg). Fin ray annuli were measured and tested using knowledge of known age; known locations from physical capture events were assigned to age at capture.

Results

Strontium isotope analysis was unable to detect differences in the Kootenai River and Kootenay Lake using water or fin ray samples. The Kootenai River and Kootenay Lake were distinguishable using trace element data from water samples, but not fin rays. The discrepancy with trace elements appears to be associated with the physiology of fin ray growth.

Discussion

Although this study did not meet its original objective of describing the movement of White Sturgeon in the Kootenai River basin from fin ray microchemistry, our results provide insight into the potential influence of physiology on microchemistry analysis. In particular, fin ray microchemistry of slow-growing fishes may be possible in freshwater systems with further investigation into the physiological processes associated with growth and the incorporation of elements into calcified structures.
Pathological response following neoadjuvant immunotherapy and imaging characteristics in dMMR/MSI-H locally advanced colorectal cancer
Authors: Deng Zijian , Luo Yajun , Chen Xiaoli , Pan Tao , Rui Yuanyi , Hu Hai , Yan Jin , Zhang Ke , Luo Cheng , Song Bo
Published: September 2024

Background

In recent years, there has been significant research interest in immunotherapy for colorectal cancer (CRC). Specifically, immunotherapy has emerged as the primary treatment for patients with mismatch repair gene defects (dMMR) or microsatellite highly unstable (MSI-H) who have colorectal cancer. Yet, there is currently no data to support the practicality and safety of neoadjuvant immunotherapy for colorectal cancer with dMMR or MSI-H. Therefore, a study was conducted to identify the postoperative pathology, safety profile, and imaging features of patients with dMMR or MSI-H CRC following neoadjuvant immunotherapy.

Methods

The retrospective study was carried out on patients with locally advanced or metastatic CRC who received immunotherapy at Sichuan Cancer Hospital, with approval from the hospital’s ethics committee. The study aimed to assess the short-term effectiveness of immunotherapy by focusing on pathological complete response (pCR) as the primary outcome, while also considering secondary endpoints such as objective response rate, disease-free survival, and safety profile.

Results

Twenty patients with dMMR/MSI-H CRC who underwent neoadjuvant immunotherapy as part of the treatment were enrolled between May 2019 and February 2024 at Sichuan Cancer Hospital. Out of these patients, eight patients received PD-1 blockade monotherapy as neoadjuvant treatment, while 12 were administered a combined therapy of anti-CTLA-4 and anti-PD-1. 12 patients received Nivolumab plus Ipilimumab regimen and 8 patients received PD-1 blockades (2 patients were Pembrolizumab, 2 patients were Sintilimab, 4 patients were Tislelizumab) monotherapy. Additionally, 19 patients underwent surgery after immunotherapy and of these, 15 (75.0%) achieved complete pathological response (pCR), 8 (66.7%) achieved the same on Nivolumab plus Ipilimumab immunotherapy while 7 (87.5%) achieved on PD-1 antibody monotherapy. The overall response rate (ORR) was 75%, with 45.0% of patients experiencing grade I/II immunotherapy-related adverse events. The most frequent adverse event observed was increased ALT i.e. 20%. Notably, no postoperative complications were observed.

Conclusion

Based on the findings, neoadjuvant immunotherapy for colorectal cancer may be both safe and effective in clinical practice. Furthermore, the study suggested that dual immunotherapy could potentially increase the immunotherapy cycle and contribute to a superior pCR rate. However, the conclusion emphasized the need for further prospective clinical trials to validate these results.
Detecting local variations across metazoan communities in back-reef depressions of Reunion Island (Mascarene Archipelago) through environmental DNA survey
Authors: Cowart Dominique A. , Chevrier Thomas , Nieblas Anne-Elise , Chanut Jérémie , Bernard Serge , Bonhommeau Sylvain
Published: September 2024

The back-reef depressions, or lagoons, of Reunion Island (western Indian Ocean) host a high abundance of organisms living amongst the coral reefs and are critical sites for artisanal fishing, tourism, and shoreline stability for the island. Over time, increasing degradation of Reunionese reefs has been observed due to overexploitation, beach erosion and eutrophication. Efforts to mitigate the impact of these pressures on aquatic organisms include biodiversity surveys primarily performed through visual censuses that can be logistically complex and may unintentionally overlook organisms. Surveys integrating environmental DNA (eDNA) collections have provided rapid biodiversity assessments, while helping to circumvent some limitations of visual surveys. The present study describes the results of an exploratory eDNA survey, which aims to characterize metazoan communities of four Reunionese lagoons located along the west coast of the island. As eDNA surveys first require deliberate study design and optimization for each new context, we sought to establish a modernized workflow implementing specialized equipment to collect and preserve samples to facilitate future studies in these lagoons. During the austral summer of 2023, samples were pumped directly from surface and bottom depths at each site through self-preserving filters which were then processed for DNA metabarcoding using regions of the 12S ribosomal RNA (12S), small ribosomal subunit 18S (18S) and Cytochrome Oxidase I (COI) genes. The survey detected high species richness that varied by site, and in a single collection period, recovered the presence of 60 teleost families and numerous invertebrate taxa, including members of the coral faunal community that are less studied in Reunion. Distinct biological communities were observed at each site, and within a single lagoon, suggesting that these differences are due to site-specific factors (e.g., environmental variables, geographic distance, etc.). Although continued protocol optimization is needed, the present findings demonstrate the successful application of an eDNA-based survey for biodiversity assessment within Reunionese lagoons.
Structure features of Streptococcus pneumoniae FabG and virtual screening of allosteric inhibitors
Authors: Xu Kaimin , Zhong Jianliang , Li Jing , Cao Yulu , Wei Lai
Published: September 2024

Streptococcus pneumoniae, a gram-positive bacterium, is responsible for diverse infections globally, and its antibiotic resistance presents significant challenges to medical advancements. It is imperative to employ various strategies to identify antibiotics. 3-oxoacyl-[acyl-carrier-protein] reductase (FabG) is a key component in the type II fatty acid synthase (FAS II) system, which is a developing target for new anti-streptococcal drugs. We first demonstrated the function of SpFabG in vivo and in vitro and the 2 Å SpFabG structure was elucidated using X-ray diffraction technique. It was observed that the NADPH binding promotes the transformation from tetramers to dimers in solution, suggesting dimers but not tetramer may be the active conformation. By comparing the structures of FabG homologues, we have identified the conserved tetramerization site and further confirmed the mechanism that the tetramerization site mutation leads to a loss of function and destabilization through mutagenesis experiments. Starting from 533,600 compounds, we proceeded with a sequential workflow involving pharmacophore-based virtual screening, molecular docking, and binding energy calculations. Combining all the structural analysis, we identified L1, L2 and L5 as a promising candidate for SpFabG inhibitor, based on the most stable binding mode in comparison to other evaluated inhibitors.
Neuroplastic changes induced by long-term Pingju training: insights from dynamic brain activity and connectivity
Authors: Zhao Fangshi , Song Linlin , Chen Yule , Wang Shaoying , Wang Xiaoyi , Zhai Ying , Xu Jinglei , Zhang Zhihui , Lei Minghuan , Cai Wenjie , An Qi , Zhu Dan , Li Fengtan , Wang Chunyang , Liu Feng
Published: September 2024

Background

Traditional Chinese opera, such as Pingju, requires actors to master sophisticated performance skills and cultural knowledge, potentially influencing brain function. This study aimed to explore the effects of long-term opera training on the dynamic amplitude of low-frequency fluctuation (dALFF) and dynamic functional connectivity (dFC).

Methods

Twenty professional well-trained Pingju actors and twenty demographically matched untrained subjects were recruited. Resting-state functional magnetic resonance imaging (fMRI) data were collected to assess dALFF differences in spontaneous regional brain activity between the actors and untrained participants. Brain regions with altered dALFF were selected as the seeds for the subsequent dFC analysis. Statistical comparisons examined differences between groups, while correlation analyses explored the relationships between dALFF and dFC, as well as the associations between these neural measures and the duration of Pingju training.

Results

Compared with untrained subjects, professional Pingju actors exhibited significantly lower dALFF in the right lingual gyrus. Additionally, actors showed increased dFC between the right lingual gyrus and the bilateral cerebellum, as well as between the right lingual gyrus and the bilateral midbrain/red nucleus/thalamus, compared with untrained subjects. Furthermore, a negative correlation was found between the dALFF in the right lingual gyrus and its dFC, and a significant association was found between dFC in the bilateral midbrain/red nucleus/thalamus and the duration of Pingju training.

Conclusion

Long-term engagement in Pingju training induces neuroplastic changes, reflected in altered dALFF and dFC. These findings provide evidence for the interaction between artistic training and brain function, highlighting the need for further research into the impact of professional training on cognitive functions.
Case report: A rare case of neurocytoma of the Vth cranial nerve
Authors: Gui Yongping , Zhou Fanghua , Li Bin , Wu Bin , Huang Xingen , Zeng Zhaomu , Mei Shuhong
Published: September 2024

We report a case of neurocytoma originating from cranial nerve V. A 53-year-old female patient presented with a 20-day history of right frontotemporal facial paresthesia and pain. Magnetic resonance imaging (MRI) showed a 2.5-cm × 1.4-cm “dumbbell” enhancing lesion located in the cisternal segment of cranial nerve V with extension into Meckel’s cave, and the signal characteristics were suggestive of trigeminal neurinoma. The lesion was resected through a subtemporal middle cranial fossa approach. Intraoperative findings revealed that the tumor originated from the cisternal segment of cranial nerve V and extended into Meckel’s cave through the trigeminal foramen. No dural attachment was found. The tumor was debulked using sharp dissection and bipolar cautery under the microscope. Extraventricular neurocytomas (EVNs) are extremely rare tumors of the central nervous system. To date, only two cases of neurocytomas arising from cranial nerve VIII have been described. This paper summarizes the clinicopathological features of a case of neurocytoma originating from the cisternal segment of cranial nerve V with extension into Meckel’s cave and expounds the relevant diagnoses and treatments, which may provide a practical clinical basis and experience for the diagnosis and treatment of EVN in the future.
Evaluating pretreatment serum CA-125 levels as prognostic biomarkers in endometrial cancer: a comprehensive meta-analysis
Authors: Yu Zhong , Sun Yue , Guo Cuishan
Published: September 2024

Background

In recent years, the incidence of endometrial cancer (EC) has been rising. This meta-analysis aims to clarify the prognostic significance of serum CA-125 levels in EC.

Methods

Articles up to March 1, 2024, were systematically searched in EMBASE, Cochrane Library, PubMed, and Web of Science. This analysis pooled hazard ratios (HR) and 95% confidence intervals (CI) from qualifying studies to evaluate the association of CA-125 levels with overall survival (OS), progression-free survival (PFS), disease-free/relapse-free survival (DFS/RFS), and disease-specific survival (DSS).

Results

25 studies involving 7,716 patients were included. The analysis revealed that elevated CA-125 levels correlate with poorer OS (HR = 1.848, 95% CI: 1.571-2.175, p < 0.001). This association persisted across various study regions and sample sizes, and was notably strong in subgroups with a CA-125 cut-off value of less than 35 (HR = 2.07, 95% CI: 1.13-3.80, p = 0.019) and equal to 35 (HR = 2.04, 95% CI: 1.49-2.79, p < 0.001), and among type II pathology patients (HR = 1.72, 95% CI: 1.07-2.77, p = 0.025). Similarly, high CA-125 levels were linked to reduced PFS, particularly in subgroups with a CA-125 cut-off value less than 35 (HR = 1.87, 95% CI: 1.15-3.04, p = 0.012) and equal to 35 (HR = 4.94, 95% CI: 2.56-9.54, p < 0.001), and in endometrioid endometrial cancer patients (HR = 2.28, 95% CI: 1.18-4.40, p = 0.014). Elevated CA-125 levels were also indicative of worse DFS/RFS (HR = 2.17, 95% CI: 1.444-3.262, p < 0.001) and DSS (HR = 2.854; 95% CI: 1.970-4.133, p < 0.001).

Conclusion

Serum CA-125 levels before treatment was highly associated with prognosis of EC patients.
A novel functionally graded bilayer membrane with excellent barrier function and in vivo osteogenesis promotion for guided bone regeneration
Authors: Li Junxuan , Ding Jiaxin , Zhou Tao , Li Bolun , Wang Jingjing , Wang Hanchi , Fu Li
Published: September 2024

Introduction

Guided bone regeneration (GBR) technology has been widely used as a reliable method to address alveolar bone defects. To improve the clinical effects of GBR approach, there have been attempts to develop barrier membranes with enhanced regenerative properties. However, modifying the material and structure of GBR membranes to integrate physicochemical properties and biological activity remains challenging. The aim of this study was to develop a novel functionally graded bilayer membrane (FGBM) with a gradient structure and composition, and to evaluate its osteogenesis promotion effect for GBR.

Methods

By combining the phase inversion method and electrospinning method, functionally graded bilayer membranes (FGBM) with gradient structure and composition of poly(lactic-co-glycolic acid) (PLGA), nano-hydroxyapatite (nHA), and gelatin were fabricated in this study. The physicochemical and biological properties of the prepared FGBM, including structural and morphological characterization, mechanical properties, in vitro biodegradation, cell behaviors, and in vivo osteogenic bioactivity, were comprehensively evaluated.

Results

The findings demonstrated the successful fabrication of PLGA/nHA/gelatin FGBM with an asymmetric structure, exhibiting enhanced hydrophilic, mechanical, and degradation properties. The incorporation of gelatin not only improved the biological integration, but also enhanced the binding affinity between electrospun fiber layer and phase inversion layer. The FGBM with a 30% nHA mass fraction and a PLGA/gelatin mass ratio of 1:1 exhibited excellent barrier function and osteogenic bioactivities in vitro and in vivo.

Discussion

This work demonstrated the potential of PLGA/nHA/gelatin FGBM in bone regeneration and provided valuable insight for the development of barrier membrane.
The calcium transient coupled to the L-type calcium current attenuates cardiac alternans
Authors: Warren Mark , Poelzing Steven
Published: September 2024

Cardiac action potential (AP) alternans have been linked to the development of arrhythmia. AP alternans may be driven by AP instabilities, Ca2+ transient (CaT) instabilities, or both. The mechanisms underlying CaT driven AP alternans is well-supported experimentally, but the ionic mechanism underlying alternans driven by AP instabilities remain incompletely understood. Here we used the Ca2+ buffer BAPTA to remove the CaT and generate a model of AP alternans driven primarily by AP instabilities. In isolated rabbit ventricle myocytes, AP alternans induced by rapid pacing were either critically damped and persisted over time, overdamped and ceased over seconds, or underdamped progressing to 2:1 capture. Control cells predominantly exhibited critically damped alternans. In contrast, removing CaT with BAPTA destabilized alternans formation in a concentration dependent manner. Importantly, alternans were easier to induce in CaT free cells as evidenced by a higher alternans threshold relative to control cells. While the L-type Ca2+ channel agonist Bay K 8644 had a minor effect on alternans formation in myocytes with conserved CaT, combining the agonist with BAPTA markedly promoted the formation of underdamped alternans and increased the alternans threshold more than four-fold as compared to controls. Our data support a mechanistic model in which AP alternans are a primary self-sustained event in which the CaT serves as a dampening cue that curbs alternans development, likely via a canonical negative feedback process involving Ca2+ induced inhibition of L-type Ca2+ current.
A dual-branch and dual attention transformer and CNN hybrid network for ultrasound image segmentation
Authors: Zhang Chong , Wang Lingtong , Wei Guohui , Kong Zhiyong , Qiu Min
Published: September 2024

Introduction

Ultrasound imaging has become a crucial tool in medical diagnostics, offering real-time visualization of internal organs and tissues. However, challenges such as low contrast, high noise levels, and variability in image quality hinder accurate interpretation. To enhance the diagnostic accuracy and support treatment decisions, precise segmentation of organs and lesions in ultrasound image is essential. Recently, several deep learning methods, including convolutional neural networks (CNNs) and Transformers, have reached significant milestones in medical image segmentation. Nonetheless, there remains a pressing need for methods capable of seamlessly integrating global context with local fine-grained information, particularly in addressing the unique challenges posed by ultrasound images.

Methods

In this paper, to address these issues, we propose DDTransUNet, a hybrid network combining Transformer and CNN, with a dual-branch encoder and dual attention mechanism for ultrasound image segmentation. DDTransUNet adopts a Swin Transformer branch and a CNN branch to extract global context and local fine-grained information. The dual attention comprising Global Spatial Attention (GSA) and Global Channel Attention (GCA) modules to capture long-range visual dependencies. A novel Cross Attention Fusion (CAF) module effectively fuses feature maps from both branches using cross-attention.

Results

Experiments on three ultrasound image datasets demonstrate that DDTransUNet outperforms previous methods. In the TN3K dataset, DDTransUNet achieves IoU, Dice, HD95 and ACC metrics of 73.82%, 82.31%, 16.98 mm, and 96.94%, respectively. In the BUS-BRA dataset, DDTransUNet achieves 80.75%, 88.23%, 8.12 mm, and 98.00%. In the CAMUS dataset, DDTransUNet achieves 82.51%, 90.33%, 2.82 mm, and 96.87%.

Discussion

These results indicate that our method can provide valuable diagnostic assistance to clinical practitioners.
The benefit of rhythm-based interventions for individuals with autism spectrum disorder: a systematic review and meta-analysis with random controlled trials
Authors: Ding Xiaofen , Wu Jinlong , Li Dong , Liu Zexi
Published: September 2024

Objective

Individuals with autism spectrum disorder (ASD) exhibit impaired behavior synchronization, which is associated with social deficits. Numerous studies have demonstrated that rhythm-based interventions can effectively mitigate social deficits by promoting behavioral synchronization in individuals with ASD. Therefore, a review of the current literature is warranted in this field. The objectives of this review were to explore the effects of rhythm-based interventions on overall social skills and to study the differences in the effects of rhythm-based interventions on specific social skills.

Method

The databases PubMed, Web of Science, Scopus, and Psycinfo were systematically explored until March 2024. A total of eleven research studies, encompassing 408 participants diagnosed with ASD, were incorporated into the meta-analysis. Effect sizes (Hedges’ g) were computed for each comparison and amalgamated using random-effects models to evaluate the social skills of individuals with ASD. The methodological quality of each study was evaluated using the Physiotherapy Evidence Database scale(PEDro).

Results

Overall, some valuable observations were made. Rhythm-based interventions had a medium effect on the overall social skills for ASD (Hedges’s=0.681; 95%CI[0.075 to 1.286], P  < 0.05). Regarding domain-specific social skills, rhythm-based interventions had a large effect on social interaction (g = 1.299,95% CI [0.508 to 2.091]), a small effect on communication (g = 0.383, 95% CI [0.033 to 0.733], P  < 0.05), and a large effect on emotion (g = 1.752, 95% CI [0.294 to 3.210], P  < 0.05). However, we found a favorable but non-significant effect (g = 0.125, 95% CI [-0.039 to 0.289], P  >  0.05) of rhythm-based interventions on empathy. All study qualities were high (score≥6) using the Physiotherapy Evidence Database (PEDro) scale assessment.

Conclusion

This result indicates the importance of rhythm in the clinical rehabilitation of individuals with ASD. We suggest adding appropriate rhythmic elements to clinical interventions, particularly for individuals with ASD who are less socially competent.
Shared decision-making in post-coercion debriefing interventions in psychiatry – a scoping review
Authors: Froelich Katharina , Schürmann Jan , Huber Christian G. , Trachsel Manuel
Published: September 2024

Introduction

Debriefing is recommended after any coercive measure in psychiatry, but there are no wellestablished standards, and ist effectiveness remains unclear. Incorporating shared decision-making (SDM) into post-coercion debriefing interventions has potentially beneficial effects.

Methods

This scoping review provides an overview of the general characteristics of such interventions and the extent to which SDM elements are already used in such interventions.

Results

A total of 2562 references were identified in the scholarly databases Embase, PubMed, Web of Science, and PsycINFO. In addition, 14 articles were identified through manual searches of reference lists. 42 full-text articles were screened for eligibility, 13 articles met the eligibility criteria and were further analyzed.

Discussion

No intervention tool was found that clearly included all SDM elements. However, three elements of SDM were present at least partially in all interventions: definition and explanation of the health care problem, the clarification of the patient's values and preferences, and a decision or explicit deferral of the decision. Further research is needed to systematically examine the implementation and clinical effectiveness of post-coercion debriefing interventions, particularly regarding the inclusion of shared decision-making elements.
Soft tap water urgently needed for reducing risks of kidney stones at the rural villages in Yangxin, a poverty-alleviated county in central China
Authors: Zhao Jiaxin , Wang Mingyao , Jiang Tan , Wang Fangsi , Shi Xinyue , Zhang Yun , Xu Kun
Published: September 2024

Introduction

Clean drinking water is a necessity for maintaining public health and livelihoods. Hard water containing excessive calcium and magnesium threatens urinary health with elevated risks of kidney stones at rural villages with tap water shortages. After entitled poverty alleviation in 2019, residents of Yangxin county of Hubei Province in central China unexpected suffered shortage of tap water and additional cost of water softening. However, the impact of lack of tap water on risks of kidney stones was not yet quantified for residents at rural villages in Yangxin.

Methods

We conducted a cross-sectional analysis by testing 216 water samples from 114 families at four villages and surveying urinary health and living conditions of each householder.

Results

Lab tests showed that mean total hardness of well and tap water at each village was above 355 mg/L CaCO3, except tap water from the village with government support for industrial development. Mean softening depth was 309.3 mg/L at the families paying for filters or bottle water, and mean annual cost of water was 1,915 CNY (1 CNY = 0.14 USD) equivalent to 5.8% of mean annual household income. Mean tap water availability was only 34%, and the prevalence of kidney stones among the four villages was 33.9%, 25.0% higher than the rate of kidney stones in Hubei Province. About 60% of the families showed strong willingness to accept annual government compensation of 3,324 CNY for clean water supply, whereas only 2.6% of the families wanted to pay government for water treatment. Using logistic and random forest regression models, we identify factors associated with the prevalence of kidney stones as gender (males have 3.78 times the risks of females), age (2.42 times greater risks if 10 years older), body mass index (higher risks if overweight or obese), total hardness of drinking water (2.59 times for a 100 mg/L increase in total hardness), and tap water availability (2.42 times higher among those without tap water supply compared to those with constant supply).

Discussion

Old and obese males are more likely to be diagnosed with kidney stones, but tap water shortages and high total hardness of drinking water increase the risks for all residents. To improve urinary health and meet support needs of residents, tap water supply of soft water is urgently needed at the rural villages in Yangxin.