bluuebunny/crossref_metadata_2025 huggingface dataset:
An alternative Semi-Autotropic Hydroponics (SAH) substrate for cassava rapid propagation: A first study case
Authors: Binzunga Mamy Makumbu , Kokou Kintche , Mouritala Sikirou , Najimu Adetoro , Ibnou Dieng , Angelique Kajibwami , Mignouna Jacob , Nyende Aggrey Bernard
Published: December 2024

The expansion of Semi-Autotrophic Hydroponics technology to address the issue of multiplying and disseminating virus-free planting materials for vegetatively propagated crops is challenged by the utilization of imported substrate, namely, KlasmannTS3. In this study, we evaluated the growth parameters and cutting production of cassava genotypes during three subsequent plantlet production cycles using three single substrates, namely, KlasmannTS3 (K), vermiculite (V), and local peat (P), and three blended substrates. The blended substrates were a combination of 25% K and 75% P (K25P75), a combination of V and P at respective rates of 25% and 75% (V25P75), and respective rates of 10% and 90% (V10P90). All cuttings obtained in one plantlet production cycle were transplanted into the next. The multiplication rate of cutting from cycle 1 to 2 (R1) and cycle 2 to 3 (R2) was calculated as the ratios of the number of cuttings per the number of plantlets in each cycle. K and K25P75 led to similar R1 and R2, except with the genotype IBA961089A, where K25P75 led to a higher R1. Local peat and V solely showed similar cutting multiplication rates, and were lower than V25P75 and V10P90. Substrates with a higher cutting production also led to a higher plantlet height, leaf, and internode number. V and its combinations with local peat led to the densest plantlet root system. The performance of the substrates contrasted among the genotypes, but IBA961089A mostly outperformed the two other genotypes. We concluded that up to 75% of K and, to a lesser extent 75% of V, can be substituted by P without compromising cutting production. V and P should be combined instead of being used separately.
Change in sediment transport regime of the Keelung River in Taiwan induced by the operation of Yuanshantze flood diversion tunnel
Authors: Lee Tsung-Yu , Teng Tse-Yang , Lee Jun-Yi , Pan Yen-Wei , Chen Ming , Chiu Chi-Cheng
Published: December 2024

The impact of flood diversion channels on river sediment transport has been rarely reported. This study uses the Yuanshantze flood diversion tunnel (YFDT), which was commissioned in July 2005 in Taiwan, as an example. This study calculates the sediment transport in the Keelung River from 1997 to 2018 by using seasonal rating curves, in the form of aQb. Changes in rating curve coefficients are also analyzed to understand the impact of YFDT on sediment transport regime. The results show that after the construction of YFDT, the annual sediment transport dropped from 0.59 ± 0.47 [Mt y-1] to 0.17 ± 0.09 [Mt y-1], leading to dampened inter- and intra-annual variation. Before flood diversion, the Keelung River requires ~1% cumulative time to export 50% cumulated sediment loads, but it takes ~4.5% cumulative time after flood diversion. Exponent b decreased from 1.23±0.18 to 1.15±0.13, and log a decreased from 0.71±0.15 to 0.51±0.11, suggesting that the Keelung River is akin to a different river in terms of sediment transport regime. While the design of the diversion tunnel mainly considered its impact on flow, its impact on sediment transport is far greater than its impact on flow and should not be overlooked. Whether this new normality will affect the downstream river continuum requires continuous attention.
Sub-toxic cisplatin concentrations induce extensive chromosomal, nuclear and nucleolar abnormalities associated with high malignancy before acquired resistance develops: Implications for clinical caution
Authors: Delinassios John G. , Hoffman Robert M. , Koumakis George , Palitskaris Dimitrios , Poulatsidou Kyriaki-Nefelli , Delinasios George J.
Published: December 2024

Aim

This study investigates the impact of sub-toxic cisplatin levels on nuclear and nucleolar abnormalities and chromosome instability in HeLa cells since our current knowledge of cisplatin effects on these parameters is based on studies with high concentrations of cisplatin.

Materials and methods

HeLa cells were exposed to gradually increasing sub-toxic doses of cisplatin (0.01 to 0.2 μg/ml). Cells treated with 0.1 and 0.2 μg/ml, termed HeLaC0.1 and HeLaC0.2, were not cisplatin-resistant, only exhibiting a slightly reduced viability, and were termed “cisplatin-sensitized cells.” Giemsa and silver staining were used to detect nuclear and nucleolar abnormalities and chromosomal alterations.

Results

Notable abnormalities were observed in HeLaC0.1 and HeLaC0.2 cells after treatment with sub-toxic concentrations of cisplatin: nuclei showed abnormal shapes, blebs, micronuclei, fragmentation, pulverization, and multinucleation; nucleoli exhibited irregular shapes and increased numbers; anaphase cells showed more nucleolar organizing regions. Abnormal chromosome segregation, heightened aneuploidy (81–140 chromosomes), polyploidy, double minutes, dicentrics, chromatid exchanges, chromatid separations, pulverization, and chromosome markers were prominently noted. These abnormalities were intensified in cells pre-sensitized to 0.02 or 0.08 μg/ml cisplatin for seven days, then exposed to 0.03 or 0.1 μg/ml cisplatin for 24 hours, and finally cultured in cisplatin-free medium for 24 hours before chromosome analysis.

Conclusion

HeLa cells subjected to increasing concentrations of sub-toxic cisplatin exhibited large-scale, multiple-type abnormalities in nuclei, nucleoli, chromosomes, and chromosomal numbers, indicating genetic/chromosomal instability associated with high malignancy, before the development of cisplatin resistance. These results suggest that low doses of cisplatin administration in the clinical setting may promote malignancy and caution should be used with this type of treatment.
Influences of maternal reflective functioning on adolescents’ psychosocial adjustment: The mediating role of adolescent’s reflective functioning
Authors: Park MinKyoung , Song Hyunjoo
Published: December 2024

This study examines the relationship between maternal reflective functioning and adolescents’ reflective functioning and psychosocial adjustment. In Study 1, The Parental Reflective Functioning Questionnaire for Adolescents (PRFQ-A) and Reflective Functioning Questionnaire for Youth (RFQ-Y), multidimensional scales used to assess reflective functioning in parents and adolescents, respectively, were validated in groups of Korean adolescents and mothers. In the results, the three factors were extracted (non-mentalizing, certainty, interest/curiosity) that were similar to those from the original version of the scale. However, the items comprising the interest/curiosity factor of the Korean validated RFQ-Y differed from the original scale. Both the reliability and validity of the scale were confirmed. Study 2 examined the mediating effects of adolescent reflective function on the relationship between parental reflective functioning and adolescent psychosocial adjustment. The structural equation modeling showed a good fit for all three models (adolescents’ uncertainty/confusion, certainty, and interest/curiosity). However, some mediating effects of adolescents’ reflective function were not confirmed. In conclusion, the results indicate that maternal reflective function influences the adolescents’ psychosocial function through the mediating factor of adolescent reflective function, Unexpectedly, maternal interest/curiosity did not have a significant effect on adolescents’ reflective function and psychosocial adjustment. Based on the results, altering Korean mothers’ parenting attitudes may have a positive effect on adolescents’ psychosocial adjustment. This work highlights that further research is needed to understand parent-child relationships in terms of reflective functioning, taking into account the influence of cultural context.
Analyzing the impact of socioeconomic indicators on gender inequality in Sri Lanka: A machine learning-based approach
Authors: Kularathne Sherin , Perera Amanda , Rathnayake Namal , Rathnayake Upaka , Hoshino Yukinobu
Published: December 2024

This study conducts a comprehensive analysis of gender inequality in Sri Lanka, focusing on the relationship between key socioeconomic factors and the Gender Inequality Index (GII) from 1990 to 2022. By applying machine learning techniques, including Decision Trees and Ensemble methods, the study investigates the influence of economic indicators such as GDP per capita, government expenditure, government revenue, and unemployment rates on gender disparities. The analysis reveals that higher GDP and government revenues are associated with reduced gender inequality, while greater unemployment rates exacerbate disparities. Explainable AI techniques (SHAP) further highlight the critical role of government policies and economic development in shaping gender equality. These findings offer specific insights for policymakers to design targeted interventions aimed at reducing gender gaps in Sri Lanka, particularly by prioritizing economic growth and inclusive public spending.
Extending the Environmental influences on Child Health Outcomes (ECHO) Cohort through 2030: Rationale and study protocol
Authors: Blackwell Courtney K. , Cella David , Adair Linda , Cordero José F. , Das Suman R. , Elliott Amy J. , Hipwell Alison E. , Jacobson Lisa P. , Neiderhiser Jenae M. , Stanford Joseph B. , Wright Rosalind J. , Gershon Richard ,   on behalf of the ECHO Cohort Consortium
Published: December 2024

Early life environmental exposures, even those experienced before conception, can shape health and disease trajectories across the lifespan. Optimizing the detection of the constellation of exposure effects on a broad range of child health outcomes across development requires considerable sample size, transdisciplinary expertise, and developmentally sensitive and dimensional measurement. To address this, the National Institutes of Health (NIH) Environmental influences on Child Health Outcomes (ECHO) Cohort Study is an observational longitudinal pediatric cohort study. In the first phase from 2016–2023, the ECHO Program built a robust platform for investigating prenatal and early life environmental exposures on child health outcomes. Now, the ECHO Program is extending longitudinal follow-up of existing ECHO participants <21 years of age and recruiting and following new pregnant participants <20 weeks gestation and their offspring through 2030. Participants will be enrolled at 72 Cohort Study Sites across all 50 US states, the District of Columbia, and Puerto Rico. Exposure assessments span the biological, chemical/physical, lifestyle, and social environment; child health outcomes focus on five broad domains: pre-, peri-, postnatal; airways; obesity; neurodevelopment; and positive health, or one’s physical, mental, and social well-being. Data and biospecimens will be collected annually through August 2030, with an expected total sample size of 60,000 children and their caregivers. The ECHO Cohort Study represents the largest national longitudinal study of children’s health in the US. Here, we describe the ECHO Cohort “Cycle 2” observational study arm and the ECHO Cohort Protocol version 3.0 (ECP v3.0), which delineates the data elements, measures, and biospecimens that all ECHO Cycle 2 Cohort Study Sites will collect and analyze.
LSTM-based estimation of lithium-ion battery SOH using data characteristics and spatio-temporal attention
Authors: Xu Gengchen , Xu Jingyun , Zhu Yifan
Published: December 2024

As the primary power source for electric vehicles, the accurate estimation of the State of Health (SOH) of lithium-ion batteries is crucial for ensuring the reliable operation of the power system. Long Short-Term Memory (LSTM), a special type of recurrent neural network, achieves sequence information estimation through a gating mechanism. However, traditional LSTM-based SOH estimation methods do not account for the fact that the degradation sequence of battery SOH exhibits trend-like nonlinearity and significant dynamic variations between samples. Therefore, this paper proposes an LSTM-based lithium-ion SOH estimation method incorporating data characteristics and spatio-temporal attention. First, considering the trend-like nonlinearity of the degradation sequence, which is initially gradual and then rapid, input features are filtered and divided into trend and non-trend features. Then, to address the significant dynamic variations between samples, especially for capacity regeneration,a spatio-temporal attention mechanism is designed to extract spatio-temporal features from multidimensional non-trend features. Subsequently, an LSTM model is built with trend features, spatio-temporal features, and actual capacity as inputs to estimate capacity. Finally, the model is trained and tested on different datasets. Experimental results demonstrate that the proposed method outperforms traditional methods in terms of SOH estimation accuracy and robustness.
Effect of curing temperature freeze–thaw failure mechanism and damage model of equal-strength air-entrained concrete
Authors: Liu YongHe , Yang Bo , Guo Aojun
Published: December 2024

The Belt and Road strategy has significantly advanced the scale of infrastructure construction in the Qinghai–Tibet Plateau permafrost area. Consequently, this demands higher requirements on the strength and frost resistance of concrete (FRC) cured under low-temperature and negative-temperature conditions. Accordingly, in this study, tests on the mechanical properties and FRC were conducted under standard curing, 5 °C curing, and −3 °C curing conditions. The pore structure characteristics of concrete subjected to freeze–thaw (F–T) damage (FTD) under different curing methods were analyzed using nuclear magnetic resonance. The study results show that when the air content is constant, the compressive strength of concrete (CSC) tends to decrease with the curing temperature. Moreover, the occurrence of an age lag phenomenon is evident. The compressive strength of concrete cured under standard curing for 28-d was comparable to that achieved by concrete cured at 5 °C curing for 56-d and at −3 °C curing for 84-d. Under the same curing conditions, the CSC decreases with increasing air content. Observations revealed that with the air content in the concrete set at 0.08%, the material’s compressive strength was at its minimum. As the number of F–T cycles increases, the concrete transverse relaxation time (T2) curve shifts to the right, and the proportion of both harmful and multi-harmful pores increases. Based on the same CSC under different curing methods, the FRC under 5 °C curing and −3 °C curing conditions is considerably lower than that under standard curing conditions. Moreover, the FRC exhibits an increasing and then a decreasing trend with increasing air content. Concrete exhibits the best frost resistance when the air content is 3.6%. It was established that an optimal range exists for air content in concrete. If the air content is too low, there is only a slight improvement in the FRC. Conversely, if the air content was excessively high, it leads to a significant decrease in frost resistance. Further, this study establishes an FTD model for concrete under 5 °C curing and −3 °C curing conditions considering the compressive strength factors of concrete under standard curing conditions for 28-d. This study is anticipated to be used as reference for determining the FRC cured under different temperatures.
Oscillatory autophagy induction is enabled by an updated AMPK-ULK1 regulatory wiring
Authors: Kapuy Orsolya , Holczer Marianna , Csabai Luca , Korcsmáros Tamás
Published: December 2024

Autophagy-dependent survival relies on a crucial oscillatory response during cellular stress. Although oscillatory behaviour is typically associated with processes like the cell cycle or circadian rhythm, emerging experimental and theoretical evidence suggests that such periodic dynamics may explain conflicting experimental results in autophagy research. In this study, we demonstrate that oscillatory behaviour in the regulation of the non-selective, stress-induced macroautophagy arises from a series of interlinked negative and positive feedback loops within the mTORC1-AMPK-ULK1 regulatory triangle. While many of these interactions have been known for decades, recent discoveries have revealed how mTORC1, AMPK, and ULK1 are truly interconnected. Although these new findings initially appeared contradictory to established models, additional experiments and our systems biology analysis clarify the updated regulatory structure. Through computational modelling of the autophagy oscillatory response, we show how this regulatory network governs autophagy induction. Our results not only reconcile previous conflicting experimental observations but also offer insights for refining autophagy regulation and advancing understanding of its mechanisms of action.
Examining the relationship between language development, executive function, and screen time: A systematic review
Authors: Bal Mazhar , Kara Aydemir Ayşe Gül , Tepetaş Cengiz Gülüzar Şule , Altındağ Ahmet
Published: December 2024

This systematic review study examines the relationship between language development, executive function, and screen time in early childhood. The early childhood period is a crucial phase for the development of the brain, during which fundamental language and executive function skills undergo rapid evolution. This review synthesizes findings from 14 peer-reviewed studies that focused on language development, executive function, and screen time together to provide a comprehensive understanding of their relationship. The findings of current study were categorized under four themes: screen content and adherence to guidelines, parent-child interaction and the family context, passive and active screen time, and attention issues. The findings suggest that interactive and educational screen content may positively influence language development and executive functions when aligned with recommended screen time guidelines. In contrast, excessive passive screen time, such as watching television, has been associated with negative impacts on cognitive and social skills, particularly affecting attention, memory, and emotional regulation. The prevalence of attention problems is found to be higher in individuals who engage in high levels of screen time. This highlights the necessity for balanced consumption of screen media. The review emphasizes the pivotal role of parent-child interaction, where high-quality engagement and verbal scaffolding during screen time can mitigate adverse effects. Furthermore, socioeconomic and cultural factors also play a significant role. Higher socioeconomic status (SES) families are better able to manage screen time and leverage educational content to support development. These findings have the potential to inform the actions of parents, educators, and policymakers. Adherence to recommended screen time guidelines can mitigate the potential negative impact on executive functions and language skills. Furthermore, the importance of limiting passive screen time and ensuring a balance between screen use and real-world interactions and play opportunities is also highlighted.
Unraveling the relationship between stress exposure and childhood anxiety: Considering accumulation, impact, and type in the first five years of life
Authors: Valdes Viviane , Craighead Linda W. , Nelson Charles A. , Bosquet Enlow Michelle
Published: December 2024

Exposure to stressful events is linked to anxiety symptoms in children, although research examining this association in the first five years of life is limited. We sought to examine the role of various aspects of family stressful experiences such as the total accumulation, impact, and type (measured longitudinally in the first five years of life) on child anxiety symptoms at age 5 years. A community sample of children and their parents (N = 399) enrolled in a longitudinal study of emotion processing were assessed when the children were infants and at ages 2 years, 3 years, and 5 years. Parents completed the Revised Life Events Questionnaire (all visits) to assess family exposures to stressful life events and the Child Behavior Checklist (5 years) to assess child emotional and behavioral symptoms. Analyses showed that total stressful events accumulated by 4 years were significantly associated with child anxiety symptoms at 5 years (r = 0.118, p = 0.045). Total stressful events accumulated at earlier time points (by 1 year, 2 years, and 3 years of age) were not significantly associated with child anxiety symptoms at 5 years. Events reported as being impactful by families appeared to be more sensitive than total events, with accumulated impactful events as early as 1 year being significantly associated with child anxiety symptoms at 5 years (r = 0.112, p = 0.042). When considering types of stressors, cumulative exposure from the prenatal period to 5 years to financial stressors (β = 0.12, p = .035) was most saliently and significantly associated with child anxiety symptoms at 5 years (after adjusting for other categories of stress such as health, interpersonal, and logistical stressors). Together, these findings suggest that stressful life events accumulated in early life, particularly those rated as impactful for the family and those related to finances, are associated with child anxiety symptoms at 5 years.
Evaluating and refining undersea cable path planning algorithms: A comparative study
Authors: Wang Tianjiao , Wang Zengfu , Moran Bill , Wang Xinyu , Zukerman Moshe
Published: December 2024

This paper compares three automated path-planning algorithms based on publicly available data. The algorithms include a Dijkstra-based algorithm (DBA) that improves on the straightforward application of Dijkstra’s algorithm, which restricts the path only to the grid edges. We present a fair and comprehensive comparison method for evaluating multiple algorithms—DBA, the Fast Marching Method (FMM), and a great circle-based method. To evaluate the performance of automated path-planning methods, we compare them based on two main criteria: (1) the total weighted cost which is a combined measure of various costs and risks of the cable path according to their weights, and (2) the algorithm’s runtime. FMM achieves a proven minimal weighted cost cable path solution given the data. This is not the case for the other two alternatives. On the other hand, DBA may have a runtime advantage over FMM. The paper discusses the sensitivity of DBA and FMM to diagonal configurations and to variation in the triangulation of the manifold, finding that DBA is more significantly affected by these factors than FMM. Furthermore, we explore how cable direction metrics can influence the performance of these methods. Through this comparative analysis, we aim to provide insights into the efficiency and effectiveness of these methods in practical scenarios and provide a useful reference for the industry in choosing the best approach for automatic cable path planning software.
Effect of concentric exercise-induced fatigue on proprioception, motor control and performance of the upper limb in handball players
Authors: Hadjisavvas Stelios , Efstathiou Michalis A. , Themistocleous Irene-Chrysovalanto , Daskalaki Katerina , Malliou Paraskevi , Giannaki Christoforos D. , Lewis Jeremy , Stefanakis Manos
Published: December 2024

Background

The last phases of a competitive game are when shoulder injuries most commonly happen, and fatigue is thought to be a major contributing factor, perhaps because of reduced proprioception and motor control. The purpose of this study was to investigate the effect of concentric fatigue on proprioception, motor control, and performance of the upper limb in handball players.

Methods

Forty-six right-handed handball players (all males, age 26.1 ± 5.54 years) were included in this test-retest laboratory experiment. Proprioception was assessed using joint reposition sense (JRS), threshold to detection of passive movement (TTDPM), muscle onset latency (MOL), motor control using Y balance test upper quarter (YBT-UQ) and performance using the athletic shoulder test (ASH) before and immediately after fatigue intervention. The fatigue protocol consisted of concentric, maximal effort, isokinetic contractions at 900/sec with sets of 30 repetitions of the shoulder external and internal rotator muscles. Fatigue was determined by a 40% decline in the peak torque over three consecutive contractions despite reinforcing feedback and encouragement.

Results

A significant increase in absolute angular error (AAE) was observed in all target angles in both external rotation (ER) and internal rotation (IR) directions (p < 0.01). In addition, there was a significant increase in TTDPM in internal rotation after fatigue intervention (p = 0.020. Variable changes were found in YBT-UQ and ASH tests. Specifically, statistically significant differences were found in anteromedial (AM) (p = 0.041), superolateral (SL) reach directions (p = 0.005), composite score (p = 0.009) in the right hand and inferolateral (IL) reach direction in the left hand (p = 0.020) in the YBT-UQ. In addition, there was a significant reduction in isometric strength (ASH test) in the I position of the right hand (p = 0.010) and all positions of the left hand (p<0.05). Furthermore, there was an increase in MOL scores after fatigue but the increase was not significant (p > 0.05).

Conclusions

Concentric fatigue of the rotator cuff muscles induces notable deficits in joint position awareness, kinesthesia, motor control, and performance of the upper extremity in elite male handball players. Although fatigue reduces reflex reaction time the effect is only marginal.
‘once they see blood then the mood for sex is spoiled’ A qualitative exploration of female sex worker’s male client views of menstruation, sex during menses and the menstrual disc
Authors: Osire Edyth , Young Sophie , Awiti Enid , Akinyi Cynthia , Otieno Fredrick , Phillips-Howard Penelope A. , Mehta Supriya D. , Mason Linda
Published: December 2024

To continue working during menses, female sex workers (FSW) may use unhygienic absorbents to hide their menstrual status. The menstrual disc may provide a solution. Little is known about men’s knowledge and views, specifically around sex during menstruation with FSW, a population who are particularly vulnerable to violence which may be heightened during menses. To identify constructs for successful and safe menstrual disc implementation we sought to identify knowledge and attitudes towards menstruation among male clients of FSW, including views on FSW menstrual disc use during intercourse. We conducted six focus group discussions comprising a total of 51 male clients of FSW, in Kisumu, Kenya, exploring their perceptions of menses and sex during menses. In preparation for future implementation of a menstrual disc intervention, we introduced the disc to participants with an information session, answering their questions and gathering their opinions on it. Thematic analysis found most clients had limited or inaccurate knowledge about menstruation, viewing blood and the menstruator, as dirty or unclean. Sources of knowledge included school, female relatives/partners, community or church. Those reporting formal education described the biological processes more accurately, while church education lent towards stigmatized perceptions. Most participants expressed negative views towards sex during menses (i.e., unpleasurable, forbidden or risky), usually stemming from misconceptions, and reported seeking FSW services because their wife / girlfriend was menstruating. Many felt deceived if a FSW was menstruating, were often aware of materials placed vaginally to absorb blood, but generally continued with the service. No clients had prior knowledge of the menstrual disc but accepted it as a safe, hygienic, and cost-effective alternative, with some voicing interest to purchase for their wife / girlfriend. We conclude there is a need to provide accurate information on menstruation to boys and men including in school curricula and faith teaching to address lack of knowledge and negativity. These findings suggest potential for adoption of menstrual discs by FSW with minimal adverse client reaction, and highlight possible partner support for women considering adopting a menstrual disc.
Association between serum endocan levels and organ failure in hospitalized patients with cirrhosis
Authors: Wejnaruemarn Salisa , Suksawatamnuay Sirinporn , Vanichanan Jakapat , Komolmit Piyawat , Treeprasertsuk Sombat , Thanapirom Kessarin
Published: December 2024

Background & aims

Acute-on-chronic liver failure is a syndrome characterized by organ failure and high short-term mortality. The lack of reliable biomarkers for the early detection of acute-on-chronic liver failure is a significant challenge. Endothelial dysfunction plays a key role in the development of organ failure. Serum endocan is a potential new biomarker for endothelial dysfunction. Therefore, this study aimed to assess the association between endocan and organ failure and 28-day mortality in patients with cirrhosis.

Methods

Hospitalized patients with cirrhosis with and without organ failure were prospectively enrolled according to the criteria of the European Association for the Study of Liver-Chronic Liver Failure consortium. The comparative performances of serum endocan, procalcitonin, and interleukin-6 for diagnosing organ failure and predicting mortality were studied.

Results

The study included 116 hospitalized patients with cirrhosis, 55 of whom had organ failure on admission. Patients with organ failure had significantly higher endocan, procalcitonin, and interleukin-6 levels than those without it. At a cut-off value of 15.8 ng/mL, endocan showed a sensitivity of 63.6% and specificity of 67.2% for the diagnosis of organ failure, with an area under the receiver operating characteristic curve of 0.65, which is comparable to procalcitonin and interleukin-6. Multivariate analysis identified serum endocan, creatinine, and total bilirubin as independent factors for organ failure in hospitalized patients with cirrhosis. Patients who died within 28 days had significantly higher baseline biomarker levels than those who survived. Liver failure, hospital-acquired infection, mechanical ventilator use, and interleukin-6 ≥37 pg/mL were independent predictors of 28-day mortality.

Conclusion

Serum endocan is associated with organ failure and is an independent risk factor of organ failure in hospitalized patients with cirrhosis.
A new view of spaces and their properties in the sense of non-Newtonian measure
Authors: Darweesh Amer , Almalki Adel , Al-Khaled Kamel , Ayyash Alaa
Published: December 2024

This study presents a novel approach to metric spaces through the lens of geometric calculus, redefining traditional structures with new operations and properties derived from non-Newtonian measures. Specifically, we develop and prove geometric versions of the Hölder and Minkowski inequalities, which provide foundational support for applying these spaces in analysis. Additionally, we establish key relationships between geometric and classical metric spaces, examining concepts such as openness, closedness, and separability within this geometric framework. By exploring topological characteristics and separability conditions in geometric metric spaces, this work enhances the understanding of metric spaces’ structural properties, offering potential applications in fields that require flexible metric adaptations, such as data science, physics, and computational geometry. This framework’s adaptability makes it relevant for scenarios where non-Euclidean or high-dimensional spaces are needed, allowing for versatile applications and extending classical metric concepts into broader analytical contexts.
The impact of wearing compression hosiery and the use of assistive products for donning and doffing: A descriptive qualitative study into user experiences
Authors: Hagedoren—Meuwissen Edith , Roentgen Uta , Zwakhalen Sandra , van der Heide Loek , van Rijn Marie Josee , Daniëls Ramon
Published: December 2024

This qualitative study aimed to describe users’ experiences and needs related to wearing, donning, and doffing compression hosiery, and the provision process of compression hosiery and associated assistive products for donning and doffing. Adults who have been advised to wear compression hosiery participated in semi-structured interviews. Existing frameworks about the provision process and acceptance of assistive technology guided the topic list. The interviews were analyzed using directed content analysis. After 19 interviews, data saturation was reached. Three main themes were revealed: (1) becoming and being a compression hosiery wearer (or not), (2) wearing compression hosiery, and (3) donning and doffing compression hosiery. In cases of acute conditions, the participants reported feeling overwhelmed when they were first prescribed compression hosiery. In contrast, those with long-term complaints felt relieved. Participants considered advantages and disadvantages and then decided whether to wear compression hosiery. Despite mentioned beneficial effects from wearing compression hosiery, participants had to cope with a range of discomforts, including pinching, straining, sagging, and heat. Additionally, participants had difficulties with the appearance, and often tried to hide the compression hosiery. They mentioned problems with donning and doffing, which can result in dependency of home or informal care, which stopped some participants from wearing. In general, participants were not aware of the full range of assistive products for donning and doffing, but were interested in them. In conclusion, wearing compression hosiery has a large impact on a person’s life because of its lack of comfort, unattractive appearance, and possible loss of independence through the need of donning and doffing support. These are expressed reasons for non-adherence, in addition to a lack of understanding of the importance of wearing and the consequences of not wearing compression hosiery. Easy-to-find independent information and more attention to donning and doffing during the fitting appointment of compression hosiery are recommended.
How does habit strength affect reactions to warning labels? evidence from Chinese consumers’ choices of carbonated beverages
Authors: Ding Yulian , Yang Yue , Sun Yangyang , Chen Kevin , Hu Lianfeng
Published: December 2024

Effectively regulating the excessive consumption of sugar-sweetened beverages (SSBs) has been an important task for public health authorities around the world. The rapid increase in SSB consumption in China necessitates robust regulations. This study employed a choice experiment to simulate the market scenario in which a text warning label was presented on SSBs. We draw a sample of 1200 participants from five major cities across China to analyze the warning label’s effectiveness in reducing urban consumers’ purchases of SSBs, with a particular emphasis on how an individual’s habit strength affects their reaction to the warning label. Our analysis unveiled varying levels of habit strength among respondents: 35.33% showed weak habits, 56.25% displayed moderate habits, and 8.42% demonstrated strong habits. The random parameters logit model results showed a significant overall decrease in SSB purchases when a warning label was present, indicated by a significant negative coefficient associated with the label. However, when examining the impact of warning labels across different habit strengths, a significant effect was observed only in the moderate habit group. Furthermore, within this group, the warning label was more effective for those with correct health knowledge and who were not overweight or obese.
Longer-term consequences of increased body checking in women at risk for eating disorders–a naturalistic experimental online study
Authors: Geiger Gina , Opladen Vanessa , Vivell Maj-Britt , Vocks Silja , Hartmann Andrea S.
Published: December 2024

Body checking is a common behavior in both the general population and individuals with body image disturbances. Cognitive-behavioral theories postulate that body checking reduces negative emotions in the short term, but over time contributes to the development and maintenance of eating disorder pathology. So far, few experimental studies have assessed these longer-term consequences, mostly under laboratory conditions, yielding inconsistent findings, and without considering individual vulnerability and specific personality traits. In a naturalistic experimental cross-over design, women with low (n = 76) vs. high (n = 103) body concern completed an online survey on trait characteristics (e.g., intolerance of uncertainty). After a two-day baseline to assess the daily amount of habitual body checking, participants underwent two three-day experimental conditions in randomized order, in which they were asked to exhibit typical vs. threefold increased body checking. Before and after conditions, participants completed state measures of eating disorder symptoms, body dissatisfaction, affect, and general pathology online. In women with high body concern, body image-related symptoms (i.e., drive for thinness, body dissatisfaction) and negative affect worsened in the increased body checking condition, whereas in the typical body checking condition, positive affect increased and no negative impact emerged. Conversely, women with low body concern remained unaffected, except for higher drive for thinness following the increased condition. Bulimic and depressive symptoms did not change in either group. The inclusion of intolerance of uncertainty from an exploratory perspective generally did not impact the results. Our findings regarding the high-risk group underscore the potential etiological relevance of body checking for body image disturbances and eating disorders. For individuals at risk and those already affected by eating disorders, it seems important to address individual body checking as early as possible within psychoeducation to prevent a presumably harmful increase in this behavior. Personality factors influencing vulnerability to body checking need to be further examined.
Nomogram model for predicting secondary infection in critically ill patients with heatstroke: A pilot study from China
Authors: Lin Guodong , Peng Hailun , Yin Bingling , Xu Chongxiao , Zhao Yueli , Liu Anwei , Guo Haiyang , Pan Zhiguo
Published: December 2024

Objective

In this retrospective analysis, we explored the clinical characteristics and risk factors of secondary infections in patients with severe heatstroke with the aim to gain epidemiological insights and identify risk factors for secondary infections.

Method

The study included 129 patients with severe heatstroke admitted to the General Hospital of the Southern Theater Command of the PLA between January 1, 2011, and December 31, 2021. Patients were divided into an infection group (n = 24) and a non-infection group (n = 105) based on infection occurrence within 48 h of intensive care unit (ICU) admission. Clinical indicators, infection indicators, and clinical outcomes within 24 h of ICU admission were collected and compared between the groups. Independent risk factors for infection in patients with severe heatstroke were analyzed using univariate and multivariate analyses. A nomogram model was constructed, evaluated, and validated.

Result

Among the 129 patients with heatstroke, 24 developed secondary infections. Infections occurred between days 3 and 10 post-ICU admission, primarily affecting the lungs. Multivariate analysis identified vasopressor use, serum creatinine level, and gastrointestinal dysfunction at admission as independent risk factors, while elevated lymphocyte count (odds ratio [OR] = 0.167; 95% confidence interval [CI] 0.049–0.572; P = 0.004) was protective against severe heatstroke. Infected patients required longer durations of mechanical ventilation (OR = 2.764; 95% CI, 1.735–4.405; P = 0.044) and total hospital stay than those in the non-infection group. The nomogram model demonstrated clinical feasibility.

Conclusion

Increased lymphocyte count is an independent protective factor against infections in patients with severe heatstroke. Vasopressor use, gastrointestinal dysfunction, and elevated serum creatinine levels are independent risk factors. These indicators can aid clinicians in assessing infection risk in patients with severe heatstroke.
Butyric acid alleviates LPS-induced intestinal mucosal barrier damage by inhibiting the RhoA/ROCK2/MLCK signaling pathway in Caco2 cells
Authors: Liu Luqiong , Chen Tong , Xie Zhenrong , Zhang Yongjin , He Chenglu , Huang Yongkun
Published: December 2024

Butyric acid (BA) can potentially enhance the function of the intestinal barrier. However, the mechanisms by which BA protects the intestinal mucosal barrier remain to be elucidated. Given that the Ras homolog gene family, member A (RhoA)/Rho-associated kinase 2 (ROCK2)/Myosin light chain kinase (MLCK) signaling pathway is crucial for maintaining the permeability of the intestinal epithelium, we further investigated whether BA exerts a protective effect on epithelial barrier function by inhibiting this pathway in LPS-induced Caco2 cells. First, we aimed to identify the optimal treatment time and concentration for BA and Lipopolysaccharide (LPS) through a CCK-8 assay. We subsequently measured Trans-epithelial electrical resistance (TEER), FITC-Dextran 4 kDa (FD-4) flux, and the mRNA expression of ZO-1, Occludin, RhoA, ROCK2, and MLCK, along their protein expression levels, and average fluorescence intensity following immunofluorescence staining. We then applied the ROCK2 inhibitor Y-27632 and reevaluated the TEER, FD-4 flux, and mRNA, and protein expression of ZO-1, Occludin, RhoA, ROCK2, and MLCK, as well as their distribution in Caco2 cells. The optimal treatment conditions were determined to be 0.2 mmol/L BA and 5 μg/mL LPS for 24 hours. Compared with LPS treatment alone, BA significantly mitigated the reduction in the TEER, decreased FD-4 flux permeability, increased the mRNA expression of ZO-1 and Occludin, and normalized the distribution of ZO-1 and Occludin in Caco2 cells. Furthermore, BA inhibited the expression of RhoA, ROCK2, and MLCK, and normalized their localization within Caco2 cells. Following treatment with Y-27632, the epithelial barrier function, along with the mRNA and protein expression and distribution of ZO-1 and Occludin were further normalized upon inhibition of the pathway. These findings contribute to a deeper understanding of the potential mechanisms through which BA attenuates LPS-induced impairment of the intestinal epithelial barrier.
Alternative splicing expands the antiviral IFITM repertoire in Chinese rufous horseshoe bats
Authors: Mak Nelly S. C. , Liu Jingyan , Zhang Dan , Taylor Jordan , Li Xiaomeng , Rahman Kazi , Chen Feiyu , Datta Siddhartha A. K. , Lai Kin Kui , Shi Zhengli , Temperton Nigel , Irving Aaron T. , Compton Alex A. , Sloan Richard D.
Published: December 2024

Species-specific interferon responses are shaped by the virus-host arms race. The human interferon-induced transmembrane protein (IFITM) family consists of three antiviral IFITM genes that arose by gene duplication. These genes restrict virus entry and are key players in antiviral interferon responses. The unique IFITM repertoires in different species influence their resistance to viral infections, but the role of IFITMs in shaping the enhanced antiviral immunity of reservoir bat species is unclear. Here, we identified an IFITM gene in Chinese rufous horseshoe bat, a natural host of severe acute respiratory syndrome (SARS)-related coronaviruses, that is alternatively spliced to produce two IFITM isoforms in native cells as shown by transcriptomics. These bat IFITMs have conserved structures in vitro as demonstrated by circular dichroism spectroscopy, yet they exhibit distinct antiviral specificities against influenza A virus, Nipah virus and coronaviruses including SARS-CoV, SARS-CoV-2 and MERS-CoV. In parallel with human IFITM1-3, bat IFITM isoforms localize to distinct sites of virus entry which influences their antiviral potency. Further bioinformatic analysis of IFITM repertoires in 206 mammals reveals that alternative splicing is a recurring strategy for IFITM diversification, albeit less widely adopted than gene duplication. These findings demonstrate that alternative splicing is a key strategy for evolutionary diversification in the IFITM family. Our study also highlights an example of convergent evolution where species-specific selection pressures led to expansion of the IFITM family through multiple means, underscoring the importance of IFITM diversity as a component of innate immunity.
Mechanisms of sterilizing immunity provided by an HIV-1 neutralizing antibody against mucosal infection
Authors: Richel Elie , Cordsmeier Arne , Bauer Larissa , Fraedrich Kirsten , Vestweber Ramona , Roshani Berit , Stolte-Leeb Nicole , Ensser Armin , Stahl-Hennig Christiane , Überla Klaus
Published: December 2024

Broadly neutralizing antibodies (bnAbs) against HIV-1 have been shown to protect from systemic infection. When employing a novel challenge virus that uses HIV-1 Env for entry into target cells during the first replication cycle, but then switches to SIV Env usage, we demonstrated that bnAbs also prevented mucosal infection of the first cells. However, it remained unclear whether antibody Fc-effector functions contribute to this sterilizing immunity. Therefore, additional challenge viruses were produced that contain SIV Env and graded doses of a fusion-defective trimer of HIV-1 Env, to which the bnAb, PGT121 can bind without interfering with the SIV Env-based cell entry. After administration of either PGT121 or its mutant deficient in Fc-effector functions, rhesus macaques were intrarectally exposed to these challenge viruses and to those using either HIV-1 Env or SIV Env for entry into the first cells. Both antibodies similarly reduced infection events with the challenge virus using HIV-1 Env by a factor close to 200. Incorporating fusion-defective HIV-1 Env trimers into the particles of the challenge viruses at densities observed in primary virus isolates did not reduce SIV Env-mediated infection events. The results indicate that the sparsity of bnAb binding-sites on HIV-1 virions limits the contribution of Fc-effector functions to provide sterilizing immunity against mucosal viral infection. Hence, harnessing Fc-effector functions for sterilizing immunity against mucosal HIV-1 infection may require strategies to increase the degree of antibody opsonization.
‘Your sexual orientation based on your bias’: Queerness as a new default in online K-pop fan communities
Authors: Trzcińska Julia
Published: December 2024

Emerging literature on K-pop underscores that many international fans perceive their engagement with this cultural phenomenon as a subversive practice and a means of identity formation. Consequently, members of the LGBTQ+ community often find a sense of belonging in these fandoms that actively challenge societal norms. An analysis of K-pop content on TikTok reveals a specific trend in which queerness is frequently assumed as the default orientation for all fans, exemplified by the popular TikTok trend ‘[y]our sexual orientation based on your bias’. While primarily intended for entertainment, the volume of such videos raises questions about the complex relationship between sexual orientation and fandom affiliation. The study analysed 35 popular TikTok videos and 350 top comments using MAXQDA software to identify key themes. While K-pop fans largely embrace a spectrum view of orientation, this article also addresses concerns about queerbaiting and the insinuation that heterosexuality is incompatible with being a genuine K-pop fan. Additionally, it examines the trend’s paradoxes, such as reinforcing stereotypes while challenging others, and the superficial nature of some engagements within the discourse on K-pop fandom and sexual orientation representation.