bluuebunny/crossref_metadata_2025 huggingface dataset:
HIF1α-dependent glycolysis promotes macrophage functional activities in protecting against bacterial and fungal infection
Authors: Li Chunxiao , Wang Yu , Li Yan , Yu Qing , Jin Xi , Wang Xiao , Jia Anna , Hu Ying , Han Linian , Wang Jian , Yang Hui , Yan Dapeng , Bi Yujing , Liu Guangwei
Published: February 2018

Macrophages are important innate immune defense system cells in the fight against bacterial and fungal pathogenic infections. They exhibit significant plasticity, particularly with their ability to undergo functional differentiation. Additionally, HIF1α is critically involved in the functional differentiation of macrophages during inflammation. However, the role of macrophage HIF1α in protecting against different pathogenic infections remains unclear. In this study, we investigated and compared the roles of HIF1α in different macrophage functional effects of bacterial and fungal infectionsin vitroandin vivo. We found that bacterial and fungal infections produced similar effects on macrophage functional differentiation. HIF1α deficiency inhibited pro-inflammatory macrophage functional activities when cells were stimulated with LPS or curdlanin vitroor when mice were infected withL. monocytogenesorC. albicans in vivo, thus decreasing pro-inflammatory TNFα and IL-6 secretion associated with pathogenic microorganism survival. Alteration of glycolytic pathway activation was required for the functional differentiation of pro-inflammatory macrophages in protecting against bacterial and fungal infections. Thus, the HIF1α-dependent glycolytic pathway is essential for pro-inflammatory macrophage functional differentiation in protecting against bacterial and fungal infections.
Protective humoral and CD4+ T cellular immune responses of Staphylococcus aureus vaccine MntC in a murine peritonitis model
Authors: Yu Wei , Yao Di , Yu Simiao , Wang Xintong , Li Xiaoting , Wang Mengyao , Liu Shuo , Feng Zhenyue , Chen Xiaoting , Li Wanyu , Wang Lizi , Liu Wei , Ma Jinzhu , Yu Liquan , Tong Chunyu , Song Baifen , Cui Yudong
Published: February 2018

Staphylococcus aureus can cause different types of diseases from mild skin infections to life-threatening sepsis worldwide. Owing to the emergence and transmission of multidrug-resistant strains, developing an impactful immunotherapy especially vaccine control approach against S. aureus infections is increasingly encouraged and supported. S. aureus manganese transport protein C (MntC), which is a highly-conserved cell surface protein, can elicit protective immunity against S. aureus and Staphylococcus epidermidis. In this study, we evaluated the humoral immune response and CD4+ T cell-mediated immune responses in a mouse peritonitis model. The results showed that MntC-specific antibodies conferred an essential protection for mice to reduce invasion of S. aureus, which was corroborated via the opsonophagocytic killing assay and passive immunization experiment in mice, and moreover MntC-induced Th17 played a remarkable part in preventing S. aureus infection since the MntC-induced protective immunity decreased after neutralization of IL-17 by antibody in vivo and the Th17 adoptive transferred-mice could partly resist S. aureus challenge. In conclusion, we considered that the MntC-specific antibodies and MntC-specific Th17 cells play cooperative roles in the prevention of S. aureus infection.
Strength of tremor patches along deep transition zone of a megathrust
Authors: Kano Masayuki , Kato Aitaro , Ando Ryosuke , Obara Kazushige
Published: February 2018

Deep low frequency tremors are indicators of slow slip transients in the brittle-ductile transition zone along subducting plates. Investigation of comprehensive tremor activities is therefore an important issue for understanding the seismic/aseismic characteristics in transition zones. Here, we focus on the radiated energy from tremors to reveal the along-strike heterogeneity in the strength of tremor patches. Based on a tremor catalog that more accurately evaluates radiated energy, we examine the spatio-temporal activity of tremors accompanied by slow slip events (SSEs) in western Shikoku, southwestern Japan. The new finding of this study is that the energy radiated from tremors is positively correlated with the speed of tremor migration front and the slip rate along the plate interface during a SSE. This can be qualitatively explained by a stress diffusion model, which consists of along-strike heterogeneities in the effective strength of tremor patches embedded in a ductile shear zone. This effective strength heterogeneity is supported by a lateral variation in the stress drop of a SSE; it is consistent with the fluid pressure distribution along the plate boundary fault and the tidal sensitivity of tremors. Accurate evaluation of tremor activities, especially the radiated energy, can be used to infer the spatial distribution of the strength of tremor patches in transition zones worldwide.
Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
Authors: Sheets Timothy P. , Park Ki-Eun , Park Chi-Hun , Swift Steven M. , Powell Anne , Donovan David M. , Telugu Bhanu P.
Published: February 2018

The domestic pig is an attractive model for biomedical research because of similarities in anatomy and physiology to humans. However, key gaps remain in our understanding of the role of developmental genes in pig, limiting its full potential. In this publication, the role of NEUROGENIN 3 (NGN3), a transcription factor involved in endocrine pancreas development has been investigated by CRISPR/Cas9 gene ablation. Precomplexed Cas9 ribonucleoproteins targeting NGN3 were injected into in vivo derived porcine embryos, and transferred into surrogate females. On day 60 of pregnancy, nine fetuses were collected for genotypic and phenotypic analysis. One of the piglets was identified as an in-frame biallelic knockout (Δ2/Δ2), which showed a loss of putative NGN3-downstream target genes: NEUROD1 and PAX4, as well as insulin, glucagon, somatostatin and pancreatic polypeptide-Y. Fibroblasts from this fetus were used in somatic cell nuclear transfer to generate clonal animals to qualify the effect of mutation on embryonic lethality. Three live piglets were born, received colostrum and suckled normally, but experienced extreme weight loss over a 24 to 36-hour period requiring humane euthanasia. Expression of pancreatic endocrine hormones: insulin, glucagon, and somatostatin were lost. The data support a critical role of NGN3 in porcine endocrine pancreas development.
Morphological evidence of telocytes in human synovium
Authors: Rosa Irene , Marini Mirca , Guasti Daniele , Ibba-Manneschi Lidia , Manetti Mirko
Published: February 2018

A new cell type named telocyte (i.e. cell with distinctive prolongations called telopodes) has recently been identified in the stroma of various organs in humans. However, no study has yet reported the existence of telocytes in the synovial membrane of diarthrodial joints. This work was therefore undertaken to search for telocytes in the normal human synovium using transmission electron microscopy, immunohistochemistry and immunofluorescence. Ultrastructural analyses demonstrated the presence of numerous spindle-shaped telocytes in the whole synovial sublining layer. Synovial telocytes exhibited very long and thin moniliform telopodes and were particularly concentrated at the boundary between the lining and sublining layers and around blood vessels. Light microscopy confirmed the presence of CD34-positive telocytes in the aforementioned locations. Moreover, synovial telocytes coexpressed CD34 and platelet-derived growth factor receptor α. Double immunostaining further allowed to unequivocally differentiate synovial telocytes (CD34-positive/CD31-negative) from vascular endothelial cells (CD34-positive/CD31-positive). The in vitro examination of fibroblast-like synoviocyte primary cultures revealed the coexistence of different cell types, including CD34-positive telocytes projecting typical moniliform telopodes. In conclusion, our work provides the first evidence that telocytes do exist in the human synovium and lays the groundwork for future studies on synovial telocytes in a variety of degenerative and destructive joint diseases.
Development of a numerical model to predict physiological strain of firefighter in fire hazard
Authors: Su Yun , Yang Jie , Song Guowen , Li Rui , Xiang Chunhui , Li Jun
Published: February 2018

This paper aims to develop a numerical model to predict heat stress of firefighter under low-level thermal radiation. The model integrated a modified multi-layer clothing model with a human thermoregulation model. We took the coupled radiative and conductive heat transfer in the clothing, the size-dependent heat transfer in the air gaps, and the controlling active and controlled passive thermal regulation in human body into consideration. The predicted core temperature and mean skin temperature from the model showed a good agreement with the experimental results. Parametric study was conducted and the result demonstrated that the radiative intensity had a significant influence on the physiological heat strain. The existence of air gap showed positive effect on the physiological heat strain when air gap size is small. However, when the size of air gap exceeds 6 mm, a different trend was observed due to the occurrence of natural convection. Additionally, the time length for the existence of the physiological heat strain was greater than the existence of the skin burn under various heat exposures. The findings obtained in this study provide a better understanding of the physiological strain of firefighter and shed light on textile material engineering for achieving higher protective performance.
A multifunctional nanoplatform for cancer chemo-photothermal synergistic therapy and overcoming multidrug resistance
Authors: Peng Yunmei , Nie Junpeng , Cheng Wei , Liu Gan , Zhu Dunwan , Zhang Linhua , Liang Chaoyu , Mei Lin , Huang Laiqiang , Zeng Xiaowei
Published: February 2018

A multifunctional nanoplatform could overcome multidrug resistance and showed cancer chemo-photothermal synergistic therapy with the near-infrared irradiation.
Assembly and activation of supported cobalt nanocrystal catalysts for the Fischer–Tropsch synthesis
Authors: van Deelen T. W. , Su H. , Sommerdijk N. A. J. M. , de Jong K. P.
Published: February 2018

Low-temperature oxidation of cobalt nanocrystals is the preferred treatment to obtain the most uniformly distributed and active Fischer–Tropsch synthesis catalyst.
SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range
Authors: Ermakova Y. G. , Pak V. V. , Bogdanova Y. A. , Kotlobay A. A. , Yampolsky I. V. , Shokhina A. G. , Panova A. S. , Marygin R. A. , Staroverov D. B. , Bilan D. S. , Sies H. , Belousov V. V.
Published: February 2018

We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pKa, which responds to pH changes in different cellular compartments.
A diarsagermylene and a diarsastannylene stabilised by arene⋯Ge/Sn interactions
Authors: Izod Keith , Evans Peter , Waddell Paul G.
Published: February 2018

Unusual arene⋯Ge/Sn interactions stabilise the rare diarsatetrylenes 8Ge/Sn by up to 181 kJ mol−1.
Bulk and surface properties of metal carbides: implications for catalysis
Authors: Quesne Matthew G. , Roldan Alberto , de Leeuw Nora H. , Catlow C. Richard A.
Published: February 2018

We present a comprehensive study of the bulk and surface properties of transition metal carbides with rock salt structures and discuss their formation energies, electronic structure and potential catalytic activity.
Fingerprints of electronic, spin and structural dynamics from resonant inelastic soft X-ray scattering in transient photo-chemical species
Authors: Norell Jesper , Jay Raphael M. , Hantschmann Markus , Eckert Sebastian , Guo Meiyuan , Gaffney Kelly J. , Wernet Philippe , Lundberg Marcus , Föhlisch Alexander , Odelius Michael
Published: February 2018

Inversion-symmetry separation of electronic state manifolds in RIXS enables identification of transient species in photo-chemical dynamics.
Efficient structural elucidation of microhydrated biomolecules through the interrogation of hydrogen bond networks
Authors: Kim Yeonjoon , Kim Jaewook , Baek Kyung Yup , Kim Woo Youn
Published: February 2018

Pattern analysis of H-bond networks through a graph-theoretic method is very effective in determining the global minima of microhydrated biomolecules.
In situ valence modification of Pd/NiO nano-catalysts in supercritical water towards toluene oxidation
Authors: Meng Qingjie , Liu Jiajia , Weng Xiaole , Sun Pengfei , Darr Jawwad A. , Wu Zhongbiao
Published: February 2018

Pd0 is more active than PdOx in toluene oxidation owing to its capability of activating gaseous oxygen at low temperatures and returning to the original state even with excess O2.
Stability and mobility of supported Nin (n = 1–10) clusters on ZrO2(111) and YSZ(111) surfaces: a density functional theory study
Authors: Cadi-Essadek Abdelaziz , Roldan Alberto , de Leeuw Nora H.
Published: February 2018

We have used spin polarized density functional theory (DFT) to evaluate the geometrical resilience of Ni clusters on ZrO2(111) and YSZ(111).
Conversion of glucose into 5-hydroxymethylfurfural catalyzed by acid–base bifunctional heteropolyacid-based ionic hybrids
Authors: Zhao Pingping , Zhang Yunyun , Wang Yong , Cui Hongyou , Song Feng , Sun Xiuyu , Zhang Lipeng
Published: February 2018

Acid–base bifunctional ionic hybrids were employed as catalysts for glucose dehydration to HMF via a unique sequential hydrogen transfer route.
Direct transformation of bijels into bicontinuous composite electrolytes using a pre-mix containing lithium salt
Authors: Cai Dongyu , Richter Felix H. , Thijssen Job H. J. , Bruce Peter G. , Clegg Paul S.
Published: February 2018

This work demonstrates a controllable strategy for designing bicontinuous composite electrolytes.
An alternative route to single ion conductivity using multi-ionic salts
Authors: Chereddy Sumanth , Chinnam Parameswara Rao , Chatare Vijay , diLuzio Stephen Patrick , Gobet Mallory P. , Greenbaum Steven G. , Wunder Stephanie L.
Published: February 2018

Polyoligomeric silsesquioxanes with eight (LiNSO2CF3) groups can be dissolved at very high loadings into tetraglyme, forming solvent-in-salt electrolytes, and stable colloids with increasing amount of tetraglyme. Li+ions can migrate by diffusive or coordinated hopping motions. HightLi+and conductivities are obtained.
Spontaneous disproportionation of lithium biphenyl in solution: a combined experimental and theoretical study
Authors: Bondarchuk Sergey V. , Carrera Manuel , de la Viuda Mónica , Guijarro Albert
Published: February 2018

Spontaneous disproportionation of lithium biphenyl radical anion into a lithium biphenyl dianion plus neutral biphenyl, according to 2LiBiph ⇄ Li2Biph + Biph, has been evidenced experimentally by UV-vis spectroscopy and backed up theoretically using TDDFT methods.
Metal ion complexes of nucleoside phosphorothioates reflecting the ambivalent properties of lead(ii)
Authors: Sigel Astrid , Operschall Bert P. , Sigel Roland K. O. , Sigel Helmut
Published: February 2018

The lead(ii)-lone pair leads to ambivalency: hemidirected (distorted, non-spherical) coordination spheres result from electronegative O-coordination and holodirected (symmetric, spherical) ones from less electronegative S-coordination.
Facile synthesis of pyrite (FeS2/C) nanoparticles as an electrode material for non-aqueous hybrid electrochemical capacitors
Authors: Pham Duong Tung , Baboo Joseph Paul , Song Jinju , Kim Sungjin , Jo Jeonggeun , Mathew Vinod , Alfaruqi Muhammad Hilmy , Sambandam Balaji , Kim Jaekook
Published: February 2018

FeS2/C electrode of a non-aqueous hybrid electrochemical capacitor (NHEC FeS2/C||AC) demonstrated competitive energy/power densities and stable long-term cycling.
Holey nickel hydroxide nanosheets for wearable solid-state fiber-supercapacitors
Authors: Shi Peipei , Chen Rong , Li Li , An Jianing , Hua Li , Zhou Jinyuan , Liu Bin , Chen Peng , Huang Wei , Sun Gengzhi
Published: February 2018

Ultrathin and holey nickel hydroxide nanosheets are synthesized and embedded in carbon nanotube fiber for high performance wearable supercapacitors.
Application of ordered nanoparticle self-assemblies in surface-enhanced spectroscopy
Authors: Chen Jiaming , Guo Longhua , Qiu Bin , Lin Zhenyu , Wang Tie
Published: February 2018

In this review, we focused on the mechanisms of ordered nanoparticle assemblies and the strategies and applications of ordered assemblies for surface-enhanced spectroscopy.
Electron-transporting foldable alternating copolymers of perylenediimide and flexible macromolecular chains
Authors: Sakurai Tsuneaki , Orito Naomi , Nagano Shusaku , Kato Kenichi , Takata Masaki , Seki Shu
Published: February 2018

Folding behaviours in solution, solid-state structure, and electron mobility are clarified for newly-synthesized alternating copolymers of perylenediimide and flexible chains.
Preparation of magnesium silicate/carbon composite for adsorption of rhodamine B
Authors: Sun Zhiwei , Duan Xinhui , Srinivasakannan C. , Liang Jinsheng
Published: February 2018

The surface area and pore structure, electrostatic interaction and functional groups are the main adsorption mechanisms.
The study of non-Newtonian nanofluid with hall and ion slip effects on peristaltically induced motion in a non-uniform channel
Authors: Abdelsalam Sara I. , Bhatti M. M.
Published: February 2018

In this study, we considered the unsteady peristaltic motion of a non-Newtonian nanofluid under the influence of a magnetic field and Hall currents.
Electrolytic extraction of dysprosium and thermodynamic evaluation of Cu–Dy intermetallic compound in eutectic LiCl–KCl
Authors: Han Wei , Li Zhuyao , Li Mei , Gao Yinyi , Yang Xiaoguang , Zhang Milin , Sun Yang
Published: February 2018

The electrochemical reduction of dysprosium(iii) was studied on W and Cu electrodes in eutectic LiCl–KCl by transient electrochemical methods.
High-throughput identification of G protein-coupled receptor modulators through affinity mass spectrometry screening
Authors: Qin Shanshan , Meng Mengmeng , Yang Dehua , Bai Wenwen , Lu Yan , Peng Yao , Song Gaojie , Wu Yiran , Zhou Qingtong , Zhao Suwen , Huang Xiping , McCorvy John D. , Cai Xiaoqing , Dai Antao , Roth Bryan L. , Hanson Michael A. , Liu Zhi-Jie , Wang Ming-Wei , Stevens Raymond C. , Shui Wenqing
Published: February 2018

High-throughput identification of GPCR modulators through affinity MS screening.
Direct evidence of charge separation in a metal–organic framework: efficient and selective photocatalytic oxidative coupling of amines via charge and energy transfer
Authors: Xu Caiyun , Liu Hang , Li Dandan , Su Ji-Hu , Jiang Hai-Long
Published: February 2018

For the first time, the photoexcited charge separation in a metal–organic framework has been evidenced with clear ESR signals, based on efficient and selective photocatalytic oxidative coupling of amines.
DNA-templated copper nanoparticles for voltammetric analysis of endonuclease activity
Authors: Chen Xifeng , Yang Dawei , Tang Yuguo , Miao Peng
Published: February 2018

A voltammetric sensor for the detection of endonuclease activity is constructed based on DNA-templated copper nanoparticles.
Raman spectroscopic library of medieval pigments collected with five different wavelengths for investigation of illuminated manuscripts
Authors: Marucci G. , Beeby A. , Parker A. W. , Nicholson C. E.
Published: February 2018

Stepwise identification of medieval pigments by Raman spectra data and excitation source.
Thermo-responsive recoverable polymeric inhibitors for the resolution of racemic amino acids
Authors: Ye Xichong , Zhang Jie , Cui Jiaxi , Wan Xinhua
Published: February 2018

Thermoresponsive polymeric inhibitors efficiently mediate the stereoselective crystallization of asparagine monohydrate and can be recollected by simply elevating the temperature.
Stereoselective synthesis of α-methyl and α-alkyl ketones from esters and alkenesviacyclopropanol intermediates
Authors: Barysevich Maryia V. , Kazlova Volha V. , Kukel Aliaksandr G. , Liubina Aliaksandra I. , Hurski Alaksiej L. , Zhabinskii Vladimir N. , Khripach Vladimir A.
Published: February 2018

Diastereoselective Kulinkovich hydroxycyclopropanation of alkenes with esters and a protocol for the isomerization of chiral cyclopropanols to α-methyl ketones have been developed.
Spin coupling interactions in CC or B–B-cored porphyrin-mimetic graphene patch nitroxide diradicals
Authors: Song Meiyu , Song Xinyu , Bu Yuxiang
Published: February 2018

DFT calculations prove the intriguing spin coupling characteristics in CC or B–B-cored porphyrin-mimetic graphene patch nitroxide diradicals.
Insights into the interfacial strengthening mechanisms of calcium-silicate-hydrate/polymer nanocomposites
Authors: Zhou Yang , Hou Dongshuai , Geng Guoqing , Feng Pan , Yu Jiao , Jiang Jinyang
Published: February 2018

The interfacial connections between the organic and inorganic phases contribute to higher ductility nanocomposites.
Charge carrier mobility of disordered organic semiconductors with correlated energetic and spatial disorder
Authors: Kaiser Waldemar , Albes Tim , Gagliardi Alessio
Published: February 2018

Kinetic Monte Carlo study of the impact of spatial and energetic disorder on charge mobility, current distribution and transport energy in organic semiconductors.
Valorization of 2,5-furandicarboxylic acid. Diels–Alder reactions with benzyne
Authors: Serum Eric M. , Selvakumar Sermadurai , Zimmermann Nicolas , Sibi Mukund P.
Published: February 2018

Biomass-derived 2,5-furandicarboxylic acid was valorized by conversion to 1,4-naphthalenedicarboxylic acid via benzyne-cycloaddition and reductive aromatization in 66% overall yield (four steps).
Investigation on the physicochemical properties of Ce0.8Eu0.1M0.1O2−δ (M = Zr, Hf, La, and Sm) solid solutions towards soot combustion
Authors: Vinodkumar T. , Mukherjee Deboshree , Subrahmanyam Ch. , Reddy Benjaram M.
Published: February 2018

The physicochemical properties of Ce–Eu-oxides were greatly improved by the introduction of trivalent or tetravalent metal cations into their lattice.
Fabrication of sub-nanometer pores on graphene membrane for ion selective transport
Authors: Qi Han , Li Zhongwu , Tao Yi , Zhao Weiwei , Lin Kabin , Ni Zhenhua , Jin Chuanhong , Zhang Yan , Bi Kedong , Chen Yunfei
Published: February 2018

Oxygen plasma-treated graphene membranes can selectively transport protons, Na+ and Cl− ions, whereas argon plasma-treated graphene membranes cannot.
Synthetic polyprenol-pyrophosphate linked oligosaccharides are efficient substrates for mycobacterial galactan biosynthetic enzymes
Authors: Xue Xiaochao , Zheng Ruixiang Blake , Koizumi Akihiko , Han Ling , Klassen John S. , Lowary Todd L.
Published: February 2018

Synthetic glycosyl polyprenol phosphates are substrates for enzymes required for cell wall assembly in mycobacteria, including the organism that causes tuberculosis.
Facile fabrication of polyurethane-based graphene foam/lead zirconate titanate/polydimethylsiloxane composites with good damping performance
Authors: Zhang Chunmei , Chen Yujie , Li Hua , Xue Wenchao , Tian Ran , Dugnani Roberto , Liu Hezhou
Published: February 2018

Using three-dimensional graphene foam as the conductive phase, the temperature range where tan δ > 0.3 for a piezo-damping PGPP composite is broadened to −70 to 100 °C.
Theoretical study of the oxidation reactions of sulfurous acid/sulfite with ozone to produce sulfuric acid/sulfate with atmospheric implications
Authors: Sheng Fang , Jingjing Liu , Yu Chen , Fu-Ming Tao , Xuemei Duan , Jing-yao Liu
Published: February 2018

Herein, theoretical studies were performed on the atmospheric oxidation of sulfurous acid (H2SO3) and sulfite ions (HSO3−) by ozone (O3) to produce sulfuric acid and hydrosulfate ions.
Remarkable adsorptive removal of nitrogen-containing compounds from hydrotreated fuel by molecularly imprinted poly-2-(1H-imidazol-2-yl)-4-phenol nanofibers
Authors: Abdul-quadir M. S. , Ferg E. E. , Tshentu Z. R. , Ogunlaja A. S.
Published: February 2018

Molecularly imprinted poly-2-(1H-imidazol-2-yl)-4-phenol nanofibers fabricated via electrospinning displayed excellent selectivity adsorption capacities nitrogen containing compounds (N-compounds) in hydro-treated fuels.
Investigation of the exceptional charge performance of the 0.93Li4−xMn2O5–0.07Li2O composite cathode for Li-ion batteries
Authors: Freire M. , Diaz-Lopez M. , Bordet P. , Colin C. V. , Lebedev O. I. , Kosova N. V. , Jordy C. , Chateigner D. , Chuvilin A. L. , Maignan A. , Pralong V.
Published: February 2018

Herein, we report a detailed study on the high-energy density nanostructured Li4−xMn2O5–Li2O composite with a high discharge capacity of 355 mA h g−1, constituting the highest value reported to date for a lithium–manganese oxide electrode.
Na3V2(PO4)2F3@C dispersed within carbon nanotube frameworks as a high tap density cathode for high-performance sodium-ion batteries
Authors: Shen Chao , Long Hai , Wang Gencheng , Lu Wei , Shao Le , Xie Keyu
Published: February 2018

Micro-spherical Na3V2(PO4)2F3@C/CNTs with a high tap density of 1.2 g cm−3have been developed and display excellent performances for sodium-ion batteries.
Phosphorylation of BACH1 switches its function from transcription factor to mitotic chromosome regulator and promotes its interaction with HMMR
Authors: Li Jie , Shima Hiroki , Nishizawa Hironari , Ikeda Masatoshi , Brydun Andrey , Matsumoto Mitsuyo , Kato Hiroki , Saiki Yuriko , Liu Liang , Watanabe-Matsui Miki , Iemura Kenji , Tanaka Kozo , Shiraki Takuma , Igarashi Kazuhiko
Published: February 2018

The transcription repressor BACH1 performs mutually independent dual roles in transcription regulation and chromosome alignment during mitosis by supporting polar ejection force of mitotic spindle. We now found that the mitotic spindles became oblique relative to the adhesion surface following endogenous BACH1 depletion in HeLa cells. This spindle orientation rearrangement was rescued by re-expression of BACH1 depending on its interactions with HMMR and CRM1, both of which are required for the positioning of mitotic spindle, but independently of its DNA-binding activity. A mass spectrometry analysis of BACH1 complexes in interphase and M phase revealed that BACH1 lost during mitosis interactions with proteins involved in chromatin and gene expression but retained interactions with HMMR and its known partners including CHICA. By analyzing BACH1 modification using stable isotope labeling with amino acids in cell culture, mitosis-specific phosphorylations of BACH1 were observed, and mutations of these residues abolished the activity of BACH1 to restore mitotic spindle orientation in knockdown cells and to interact with HMMR. Detailed histological analysis of Bach1-deficient mice revealed lengthening of the epithelial fold structures of the intestine. These observations suggest that BACH1 performs stabilization of mitotic spindle orientation together with HMMR and CRM1 in mitosis, and that the cell cycle-specific phosphorylation switches the transcriptional and mitotic functions of BACH1.
Inositol pyrophosphate synthesis by diphosphoinositol pentakisphosphate kinase-1 is regulated by phosphatidylinositol(4,5)bisphosphate
Authors: Nair Vasudha S. , Gu Chunfang , Janoshazi Agnes K. , Jessen Henning J. , Wang Huanchen , Shears Stephen B.
Published: February 2018

The 5-diphosphoinositol pentakisphosphate (5-InsP7) and bisdiphosphoinositol tetrakisphosphate (InsP8) are “energetic” inositol pyrophosphate signaling molecules that regulate bioenergetic homeostasis. Inositol pyrophosphate levels are regulated by diphosphoinositol pentakisphosphate kinases (PPIP5Ks); these are large modular proteins that host a kinase domain (which phosphorylates 5-InsP7 to InsP8), a phosphatase domain that catalyzes the reverse reaction, and a polyphosphoinositide-binding domain (PBD). Here, we describe new interactions between these three domains in the context of full-length human PPIP5K1. We determine that InsP7 kinase activity is dominant when PPIP5K1 is expressed in intact cells; in contrast, we found that InsP8 phosphatase activity prevails when the enzyme is isolated from its cellular environment. We approach a reconciliation of this disparity by showing that cellular InsP8 phosphatase activity is inhibited by C8-PtdIns(4,5)P2 (IC50 ~40 μM). We recapitulate this phosphatase inhibition with natural PtdIns(4,5)P2 that was incorporated into large unilamellar vesicles. Additionally, PtdIns(4,5)P2 increases net InsP7 kinase activity 5-fold. We demonstrate that PtdIns(4,5)P2 is not itself a phosphatase substrate; its inhibition of InsP8 phosphatase activity results from an unusual, functional overlap between the phosphatase domain and the PBD. Finally, we discuss the significance of PtdIns(4,5)P2 as a novel regulator of PPIP5K1, in relation to compartmentalization of InsP7/InsP8 signaling in vivo.
Phase‐orthogonality CDSK: a reliable and effective chaotic communication scheme
Authors: Duan Jun‐Yi , Yang Hua
Published: February 2018

To overcome the drawback of bit‐error rate (BER) performance decrement caused by intrasignal interference (ISI) in correlation‐delay‐shift‐keying (CDSK) and its enhanced schemes, in this brief, a reliable and effective chaotic communication scheme: phase‐orthogonality CDSK is proposed. In the proposed scheme, quadrature sinusoidal wavelets are alternately selected to modulate the reference signal. This operation makes the reference signal strictly orthogonal to the information‐bearing signal and the ISI component is eliminated during the demodulation. The BER performance is analytically studied and the simulations are performed in additive white Gaussian noise channel and Rayleigh multipath fading channels. Theoretical analysis and simulations show that, since there is no ISI component and better bandwidth efficiency, the proposed BER performance is much better than differential‐chaos‐shift‐keying (DCSK) and CDSK. With the length of multiframe increasing, the proposed BER performance gradually becomes better, and can even outperform reference‐adaptive CDSK and improved DCSK.
Planning the coordination of overcurrent relays for distribution systems considering network reconfiguration and load restoration
Authors: Sharma Ankita , Kiran Deep , Panigrahi Bijaya K.
Published: February 2018

Installation of branch switches and tie‐lines in the distribution system provides the flexibility of load restoration during the occurrence of a fault, i.e. N− 1 state. These switches and tie‐lines change the network topology to isolate the fault and restore the unaffected network. As a consequence, the relays may delay or fail to operate for a possible incoming fault, i.e. N− 1−1 state; therefore, this study proposes an optimal relay setting of overcurrent relays subject to N− 1 and N− 1−1 states. Laterally, the proposed model maximises the load restoration such that the network remains radial. Also, it uses a meta‐heuristic algorithm to arrive at an optimal solution. Moreover, the optimal solution is validated using GE Multilin, model‐750/760 overcurrent relay in ETAP (industrial simulation software). The efficacy of the proposed method is examined on 33‐bus and a practical 14‐bus distribution system.
Kriegers flak‐combined grid solution: coordinated cross‐border control of a meshed HVAC/HVDC offshore wind power grid
Authors: Marten Anne‐Katrin , Akmatov Vladislav , Sørensen Thomas Bentzon , Stornowski Reinhard , Westermann Dirk , Brosinsky Christoph
Published: February 2018

The Kriegers Flak combined grid solution (KF CGS) will interconnect the eastern synchronous area of Denmark and Germany by extending the existing high‐voltage alternating current (HVAC) offshore wind farm infrastructure in the Baltic Sea. In contrast to conventional point‐to‐point interconnectors, the extension creates a meshed submarine grid (MSG) which includes an interconnector and wind farm collectors to the countries using the same equipment. Denmark East as part of the Nordic system and 50 Hertz as part of the synchronous Continental European grid are asynchronous to each other, which makes a frequency transformation necessary. The interconnection will be realised by a high‐voltage direct current (HVDC) back‐to‐back (BtB) converter in voltage source converter technology located at the German end of the interconnector. The BtB and the entire HVAC MSG between Denmark and Germany will be controlled by a so‐called master controller for interconnector operation (MIO). The KF CGS will integrate offshore wind power generation, increase the security of supply and enhance integration of regionally produced renewable energy. This study describes the overall infrastructure of the KF CGS and the MIO functions. A model‐based evaluation of those shows the behaviour of the HVAC/HVDC MSG in normal operation and in the case of contingencies.
Time‐domain virtual EMI receiver model algorithm for corona‐originated electromagnetic interference of dc transmission line
Authors: Yi Yong , Chen Zhengying , Wang Liming
Published: February 2018

A time‐domain virtual electromagnetic interference (EMI) receiver model algorithm for corona‐originated EMI of dc transmission line is proposed. The presented model is based on short‐time fast Fourier transform (FFT) and digital quasi‐peak, peak and average detector algorithms together with the measured corona current. The virtual EMI receiver model algorithm is verified by comparing with measurement results of conducted interference of the three regular waveforms. The influences of computational accuracy on the conducted interference are discussed. Through utilising the laboratory‐scale corona cage to generate corona current, the 0.5 MHz excitation current is calculated by using short‐time FFT and excitation function theory. The measured and computational average value, root mean square value, quasi‐peak value and peak value of EMI receiver response to corona current sequence are obtained. It is found that computations of radio interference have a good agreement with the measured results.
Hydrophilic coating film used to drive flow in a microfluidic point‐of‐care testing (POCT) device
Authors: Liu Chong , Li Xia , Liang Chao , Zhang Kai , Chen Li , Li Jingmin
Published: February 2018

The liquid is usually driven by capillary force to flow forward in a channel network of a point‐of‐care testing device. However, it is not enough to drive the liquid flow forward that only relies on the surface tension of the chip material itself. While the liquid drops into the chip, the unstable liquid front shape may produce bubbles or the liquid flowing will even stop which will resist the signal detection. Here, a novel method, hydrophilic coating, is demonstrated. This method used an automatic device to dip the chips from the self‐prepared TiO2 sol forming the gel film on the surface of chips’ channel. Results have shown that the coating film will enhance the liquid flow performance and improve the detection precision and accuracy.
Simulation of fracture behaviour of hydrogel by discrete element method
Authors: Yang Runhuai , Gao Tianyun , Li Didi , Liang Haiyi , Xu Qingqing
Published: February 2018

The determination of fracture properties of hydrogels is of great importance while the hydrogel is used as medical scaffold or soft robotic. The simulations of the mechanical properties were used to reduce the experiment cost and improve efficiency. Here, the work presents a novel method to simulate the fracture behaviour of hydrogels based on discrete element method. Different with continuous methods, the hydrogel was characterised by number of particles. The viscoelastic model and bonding model within the particle model were applied to simulate the microscopic structure of hydrogels. By changing the critical parameters in the model, hydrogels with different mechanical properties were also simulated. Puncture compression test and uniaxial tensile test based on the model were performed. The fracture morphology, stress and strain during the tests were simulated. The results show that the model provides a novel and effective tool to simulate the fracture process of hydrogels.
CNTs modified graphitic C3N4 with enhanced visible‐light photocatalytic activity for the degradation of organic pollutants
Authors: Wu Yibing , Liao Huiwei , Li Mengqiu
Published: February 2018

Graphitic carbon nitride(C3N4)/carbon nanotubes (CNTs) composite photocatalyst was synthesised by a facile and simple method. The resulting graphitic C3N4/CNTs composite was characterised by X‐ray powder diffraction, scanning electron microscopy and ultraviolet–visible diffuse reflectance spectroscopy techniques. The results showed that the CNTs modified by polyvinylpyrrolidone could provide a large amount of space and support for the surface area of graphitic C3N4 so that the redox reaction was faster. Therefore, the photocatalytic properties of graphitic C3N4/CNTs composite could be greatly improved. Meanwhile, compared to the pure graphitic C3N4, the surface‐modified graphitic C3N4/CNTs composite showed stronger photocatalytic activity in degradation of rhodamine B under visible‐light irradiation, especially the graphitic C3N4/CNTs (0.03 wt.%). In addition, the results also showed that the photocatalytic properties of the optimised nanocomposites were not decreased obviously.
Preovulatory follicle growth dynamics indicates daily spawning synchronicity in wild-caught Atlantic horse mackerel off Portugal
Authors: Mouchlianitis Foivos Alexandros , Nunes Cristina , Costa Ana Maria , Angélico Maria Manuel , Ganias Konstantinos
Published: February 2018

The Atlantic horse mackerel, Trachurus trachurus, is a commercially important species whose daily spawning dynamics are not yet fully understood. Existence of spawning synchronicity would facilitate application of the daily egg production method for estimating the spawning biomass of the southern stock of the species in the Northeast Atlantic. The intent of this work was to study preovulatory follicle (PREF) growth dynamics in wild Atlantic horse mackerel to determine the existence of daily spawning synchronicity in the population. The size and translucency of hydrated oocytes increased significantly between early morning and late afternoon; this pattern was the same for two study years. This morpho-developmental shift led to the appearance of previously indistinguishable oil drops in ovarian whole mounts which is an easily observed microscopic descriptor of PREF growth. The results clearly suggested that PREF growth was well synchronized between Atlantic horse mackerel that were capable of spawning and provided evidence for the existence of daily spawning synchronicity in the population. Apart from sampling time of day, the size of hydrated oocytes was shown to be affected by body size: a 10 cm difference in maternal length corresponded to a 0.030 mm difference in the diameter of hydrated oocytes. In contrast, the size of early germinal vesicle migration oocytes was correlated neither to sampling time nor to maternal size which suggests that the abrupt increase in PREF size only commences after the onset of the actively spawning sub-phase.
The argan oil project: going from utopia to reality in 20 years
Authors: Charrouf Zoubida , Guillaume Dominique
Published: February 2018

The “argan oil project” is nowadays considered as an economic success that harmoniously combined sustainable development, integrated research-action and socio-economic progress. Actually, it was a long battle whose main stages are presented here. The main stages of the argan oil project include a detailed chemical study of argan oil in order to certify argan oil quality and establish an official quality norm and obtain a geographic indication, pharmacological analyses to certify cosmetic argan oil safety, and finally a strong desire to develop Moroccan rural areas by implementing women’s cooperatives and easing the women’s access to education.
Longitudinal Stent Elongation of a Tapered Lesion during Percutaneous Coronary Intervention: A Case Report
Authors: Matsumoto Sen , Sakakibara Masayuki , Nishikawa Nagahiro
Published: February 2018

The therapeutic strategy for percutaneous coronary intervention (PCI) of a tapered lesion is still being developed. Herein, we describe a 67-year-old man with stable angina who underwent elective PCI of a tapered lesion. The coronary angiogram showed diffuse, severe stenosis in the tapered left anterior descending artery. A third-generation sirolimus-eluting stent (2.5 × 24-mm Ultimaster, Terumo, Tokyo, Japan) was deployed, and the distal side was selected as the reference. Postdilatation with a balloon diameter 2.0 mm larger than the stent was needed because of the tapered lesion. We performed postdilatation from the distal side to the proximal side of the stent with a noncompliant balloon (4.5 × 8-mm Powered Lacrosse2, Goodman, Aichi, Japan). The final angiogram showed that the total stent length was 6 mm longer than the original length. Therefore, physicians should be aware of longitudinal stent elongation of a tapered lesion during PCI to ensure appropriate treatment.
Die erste Bluttransfusion beim Menschen durch Jean-Baptiste Denis im Jahre 1667 aus medizinhistorischer Perspektive
Authors: Bauer Axel
Published: February 2018

Im Juni 1667 gelang dem französischen Arzt Jean-Baptiste Denis (ca. 1635 – 1704) in Paris die erste erfolgreiche Transfusion von Lammblut auf einen Menschen. Die Auswahl des Spendertieres erfolgte gemäß der antiken Theorie der Humoralpathologie, wonach die physischen und psychischen Eigenschaften des als friedfertig geltenden Lammes auf den Empfänger übergehen sollten. Denis zog insbesondere einen Analogieschluss von der menschlichen Ernährung mit tierischem Fleisch auf die Transfusion tierischen Blutes. Das gelungene Experiment führte in den folgenden Monaten zu einem heftigen Gelehrtenstreit, der vor allem in der englischen Zeitschrift „Philosophical Transactions“ der Londoner „Royal Society“ ausgetragen wurde. Hierbei spielte der aus Deutschland stammende Herausgeber Henry Oldenburg (1618 – 1677) eine führende Rolle, indem er die angebliche historische Priorität englischer Wissenschaftler bei der Bluttransfusion hervorhob. Durch ein Verbot des Pariser Kriminalgerichts endete diese erste Phase der Übertragung von Tierblut auf den Menschen jedoch bereits im April 1668.
Psychologische Optionen zur Reaktivierung und Bindung von Blutspendern
Authors: Greffin Klara , Muehlan Holger , Sümnig Ariane , Schmidt Silke , Greinacher Andreas
Published: February 2018

Das derzeitige Verhältnis von Vollblutspendern und -empfängern
1
wird in Zukunft weiter aus dem Gleichgewicht geraten: Der demografische Wandel und die Zunahme von Spenderausschlusskriterien führen zu einer Reduktion potenzieller Vollblutspender, während der Bedarf an Blutprodukten weiterhin steigt. Aus diesem Grund wird die Rekrutierung von Neuspendern allein nicht ausreichend sein, um einen stabilen Spenderpool zu erhalten. Um die Versorgung mit Blutprodukten zukünftig trotzdem gewährleisten zu können, rücken alternative Interventionsoptionen zunehmend in den Vordergrund. Der vorliegende Artikel zeigt psychologische Optionen auf, die auf die Begrenzung des drohenden Blutkonservenmangels abzielen, und richtet dabei den Fokus auf die wichtigste Ressource
2
– den Blutspender. Dafür sollen ergänzende Reaktivierungsmaßnahmen, die der Initiierung eines erneuten Blutspendeversuchs dienen, erläutert und diskutiert werden. Anschließend wird das Konstrukt der Spenderloyalität als eine aussichtsreiche Möglichkeit der langfristigen Spenderbindung vorgestellt.
Hereditäre Hämochromatose und Blutspende: Patient oder Spender?
Authors: Deitenbeck Robert , Zeiler Thomas
Published: February 2018

Bei der hereditären Hämochromatose (HH) handelt es sich um eine genetisch bedingte Störung des Eisenstoffwechsels, die bei der Mehrzahl der Betroffenen zu einer Verminderung von Hepcidin führt, das durch Bindung an Ferroportin im Darm die Resorption von Eisen hemmt. Hieraus resultieren Eisenablagerungen in verschiedenen Organen. Die Therapie besteht in der Entfernung von in Hämoglobin gebundenem Eisen durch regelmäßigen Aderlass, weshalb Blutspenden den Krankheitsverlauf positiv beeinflussen.
Entzündungssignal RANTES / CCL5 aus dem Kieferknochen
Authors: Lechner Johann , von Baehr Volker
Published: February 2018

Hintergrund: Diese Studie behandelt die Frage, ob allgemeine und weit verbreitete zahnärztliche Eingriffe, wie Zahnextraktionen und operative Entfernung von Weisheitszähnen, zu chronischer Kieferknochenentzündung führen. Eine mangelhafte Wundheilung im Kieferknochen kann über Entzündungsmediatoren eine versteckte Ursache chronischer Erkrankungen sein.

Materialien und Methoden: Mittels Mutliplexanalyse untersuchen wir fettig-degenerativ osteonekrotisch / osteolytisch veränderten Kieferknochen (FDOK) in zwei Gruppen auf sieben Zytokine: 16 FDOK-Präparate postoperativ in Bereichen der retromolaren Weisheitszahngebiete und 19 Präparate von gesundem Kieferknochen.

Ergebnisse: Alle FDOK-Proben zeigen RANTES / CCL5 (R / C) und den Fibroblastenwachstumsfaktor (FGF)-2 als einzige stark überexprimierte Botenstoffe. Die FDOK-Proben zeigen im Mittel einen 30-fachen Überschuss von R / C und einen 20-fachen Überschuss von FGF-2 im Vergleich zu gesunden Kontrollgruppen.

Schlussfolgerungen: Da R / C in der Literatur als wichtiger Treiber von entzündlichen Erkrankungen diskutiert wird und onkogene Wirkung haben kann, verfolgen wir die Hypothese, dass FDOK infolge mangelhafter oder unvollständiger Wundheilung im Kieferknochen eine Quelle hyperaktivierter Signalwege ist. Dabei wirkt das FDOK-Areal als unentdeckter Herd einer „stillen Entzündung“. Aufgrund der R / C-Beteiligungen bei vielen Immunerkrankungen kann die chirurgische Beseitigung einer FDOK potenzielle Entwicklungen von entzündlich systemischen Erkrankungen positiv beeinflussen.
Traumatisch bedingter Kreislaufstillstand
Authors: Kreimeier Uwe , Bogner-Flatz Viktoria
Published: February 2018

Ein schweres Trauma mit Kreislaufstillstand ist eine interdisziplinäre Herausforderung. Eine verbesserte Struktur durch das TraumaNetzwerk, die Entwicklung der S3-Leitlinie für die Schwerverletztenversorgung und die optimierte Ausbildung aller Beteiligten bilden die wichtigsten aktuellen Entwicklungen.

Der Refresher fasst kompakt den aktuellen Stand der Basismaßnahmen zur Wiederbelebung, die Priorisierung der lebensrettenden Maßnahmen auf Basis der aktuellen Leitlinie zusammen und gibt einen Ausblick auf die Prognose und das mögliche Potenzial einer guten Erstversorgung.
Die Digitalisierung der Arzt-Patienten Beziehung in
Deutschland: Ein Discrete Choice Experiment zur Analyse der Patientenpräferenzen
bezüglich digitaler Gesundheitsleistungen
Authors: von Wedel Philip , Hagist Christian , Saunders Katharina
Published: February 2018

Ziel Das deutsche E-Health Gesetz soll Effizienz, Qualität und
Zugänglichkeit des deutschen Gesundheitssystems positiv beeinflussen. Diese
Studie untersucht die Präferenzen der Patienten bezüglich digitaler
Gesundheitsleistungen und vergleicht diese mit den Prioritäten des
Gesetzgebers.

Methodik Zwei Discrete Choice Experimente (DCE) wurden durchgeführt um
Präferenzen und auch die Zahlungsbereitschaft von Patienten bezüglich digitaler
Gesundheitsinformationen und Telemedizin zu erfassen.

Ergebnisse Patienten in Deutschland bevorzugen die Speicherung einer
allumfassenden Patientenakte gefolgt von Notfalldaten auf ihrer
Versichertenkarte. Einem Medikationsplan wird eine klare Absage erteilt. In
Bezug auf eine telemedizinische Betreuung ziehen Patienten die Kommunikation via
E-Mail einer Videosprechstunde oder Text-Chats vor.

Schlussfolgerung Die Ergebnisse dieser Studie lassen zum Teil erhebliche
Diskrepanzen zwischen den Prioritäten des E-Health Gesetzes und den Präferenzen
der Patienten erkennen. Dies stellt die Adaption von Patientenseite und damit
die Effektivität des Gesetzes in Frage.