bluuebunny/crossref_metadata_2025 huggingface dataset:
Typing complex meningococcal vaccines to understand diversity and population structure of key vaccine antigens
Authors: Rodrigues Charlene M. C. , Chan Hannah , Vipond Caroline , Jolley Keith , Harrison Odile B. , Wheeler Jun , Whiting Gail , Feavers Ian M. , Maiden Martin C. J.
Published: November 2018

Background:Protein-conjugate capsular polysaccharide vaccines can potentially control invasive meningococcal disease (IMD) caused by five (A, C, W, X, Y) of the six IMD-associated serogroups.  Concerns raised by immunological similarity of the serogroup B capsule, to human neural cell carbohydrates, has meant that ‘serogroup B substitute’ vaccines target more variable subcapsular protein antigens.  A successful approach using outer membrane vesicles (OMVs) as major vaccine components had limited strain coverage. In 4CMenB (Bexsero®), recombinant proteins have been added to ameliorate this problem. 

Methods:Here, scalable, portable, genomic techniques were used to investigate the Bexsero®OMV protein diversity in meningococcal populations. Shotgun proteomics identified 461 proteins in the OMV, defining a complex proteome. Amino acid sequences for the 24 proteins most likely to be involved in cross-protective immune responses were catalogued within thePubMLST.org/neisseriadatabase using a novel OMV peptide Typing (OMVT) scheme.

Results:Among these proteins there was variation in the extent of diversity and association with meningococcal lineages, identified as clonal complexes (ccs), ranging from the most conserved peptides (FbpA, NEISp0578, and putative periplasmic protein, NEISp1063) to the most diverse (TbpA, NEISp1690).  There were 1752 unique OMVTs identified amongst 2492/3506 isolates examined by whole-genome sequencing (WGS). These OMVTs were grouped into clusters (sharing ≥18 identical OMVT peptides), with 45.3% of isolates assigned to one of 27 OMVT clusters. OMVTs and OMVT clusters were strongly associated with cc, genogroup, and Bexsero®antigen variants, demonstrating that combinations of OMV proteins exist in discrete, non-overlapping combinations associated with genogroup and Bexsero®Antigen Sequence Type. This highly structured population of IMD-associated meningococci is consistent with strain structure models invoking host immune selection.

Conclusions:The OMVT scheme facilitates region-specific WGS investigation of meningococcal diversity and is an open-access, portable tool with applications for vaccine development, especially in the choice of antigen combinations, assessment and implementation.
Designing and Implementing the Technology of the «Inverted Lesson» in Higher School
Authors: Прохорова М. , Prohorova M. , Ваганова О. , Vaganova O.
Published: November 2018

The article describes the results of experimental work on applying the technology of the "inverted lesson" in higher education. The reasons and prerequisites for using the technology of the "inverted lesson" in the domestic vocational education are described, such as an increase in the proportion of independent work in educational standards and the development of the capacities of the electronic educational environment of higher education institutions. The design procedure and the content of didactic and educational-methodical materials necessary for the implementation of this technology in the educational process are described. Presented are the results of experimental work using the technology of "inverted learning" in the educational process in the direction of training 44.03.02 "Vocational training". The results of the comparison of the performance of the control and experimental groups during the semester and the results of the control measure are presented, the time for performing the tasks of the audit work is compared, conclusions are drawn about the high potential of the "inverted lesson" technology as a way of organizing the independent work of students with a wide use of the electronic educational medium of the university. The obtained results are confirmed by the data of the survey of the students of the experimental group who highly appreciated the effectiveness of the "inverted lesson" technology.
Dynamics of Approaches to Positioning the Image of the Mayor of Moscow from 2016 to 2018
Authors: Моисеева О. , Moiseeva O. , Кожемякина Т. , Kozhemyakina T.
Published: November 2018

The article analyzes the dynamics of approaches to positioning the image of the mayor of Moscow for the period from 2016 to 2018. The data obtained in the framework of final qualifying studies in 2016 and 2018 are compared in the direction of preparation "Advertising and public relations". The change of positioning of the image of the mayor of Moscow from the image of “manager, builder” in 2016 to a more socially oriented image of a “human-faced” policy during the election campaign in 2018 is considered.
You Can't Have It All: The Experience of Academic Hospitalists During Pregnancy, Parental Leave, and Return to Work
Authors: Gottenborg Emily , Maw Anna , Ngov Li‐Kheng , Burden Marisha , Ponomaryova Anastasiya , Jones Christine D.
Published: November 2018

BACKGROUND

The United States lags behind most other countries regarding the support for working mothers and parental leave. Data are limited to describe the experience of female hospital medicine physicians during pregnancy, parental leave, and their return to work in academic hospital medicine.

METHODS

We conducted a qualitative descriptive study including interviews with 10 female academic hospitalists chosen from institutions across the country that are represented in Society of Hospital Medicine (SHM) Committees. Interview guides were based on the following domains: experience in pregnancy, parental leave, and return to work. Interviews were recorded, transcribed verbatim, and analyzed using a general inductive approach to theme analysis using the ATLAS.ti software (Scientific Software Development GmbH, Berlin, Germany).

PRIMARY OUTCOME

Women in hospital medicine experience the following six common challenges in their experience as new parents, each of which has the potential to impact their career trajectory, wellness, and are associated with areas for institutional improvement: (1) access to paid parental leave, (2) physical challenges, (3) breastfeeding, (4) career opportunities, (5) colleague responses, and (6) empathy in patient care.
MENINAS E CRIMINALIDADE: UM ESTUDO DE CASO DO CENTRO DE REEDUCAÇÃO SOCIAL SÃO JERÔNIMO (CRSSJ)
Authors: Barreto Ana Flávia Arruda Lanna
Published: November 2018

Este artigo trata-se de um estudo de caso acerca da criminalidade de adolescentes que cumpriam medidas socioeducativas de internação no Centro de Reeducação Social São Jerônimo (CRSSJ), em Belo Horizonte, Minas Gerais. É descrito a história da unidade, sua estrutura, seus principais objetivos, rotina de atividades e funções do corpo técnico e administrativo. Além disso, são analisadas as dificuldades enfrentadas na unidade socioeducativa, conceitos de violência, principais valores das adolescentes.  O estudo sobre a realidade observada nos possibilitou conhecer, mesmo que parcialmente, o universo moral e social das adolescentes em conflito com a lei, seus medos, expectativas e modos de vida que refletem a violência por elas praticada e vivenciada. Percebe-se, a partir da análise de dados qualitativos e quantitativos recolhidos em pesquisa empírica, que as adolescentes são submetidas ao uso indiscriminado da violência, a ausência de mecanismos de sociabilidade e identidade social, a inexistência de valores humanísticos norteadores da formação humana. Essas condições têm levado a ocorrência de ações arbitrárias e criminosas capazes de romper com o humano e valorizar a violência como instrumento de poder, dominação e exclusão.
A POLÍTICA DE INCLUSÃO DOS CATADORES DE RESÍDUOS SÓLIDOS: UM ESTUDO NA CIDADE DE FORTALEZA.
Authors: Moreira Gomes Ana Virginia , Aragão Neto Francisco de Assis
Published: November 2018

O objetivo do presente artigo é examinar se políticas de inclusão dos catadores de resíduos em sistemas de coleta e destinação final ambientalmente adequada de materiais é acompanhada de direitos que garantam a melhoria de suas condições de trabalho. Trata-se de uma pesquisa de campo, de natureza qualitativa, levada a efeito por meio de entrevistas com catadores em dois empreendimentos solidários do município de Fortaleza. As visitas realizadas indicam que, além das condições de trabalho degradantes, os trabalhadores organizados não auferem o salário mínimo mensal. A inclusão dos catadores nos sistemas de gerenciamento de resíduos por normas ambientais não lhes assegura nenhum direito trabalhistas. Essa política continua a possibilitar a utilização de um trabalhador vulnerável para realizar uma atividade de competência municipal. O estudo conclui que a melhoria das condições de trabalho dos catadores em um patamar de dignidade e segurança ocorrerá somente quando os sujeitos que se beneficiam da atividade desses trabalhadores se responsabilizem por essa melhoria.
A constitucionalização e a refundação da Teoria do Direito: novos dilemas
Authors: Heinen Luana Renostro , Mangili Laurindo Marcel
Published: November 2018

O formalismo predominou na Teoria do Direito até o final do século XIX. Com a constitucionalização do Direito vivenciada após a Segunda Guerra Mundial ocorreram mudanças significativas na Teoria do Direito, pois o direito se tornou cada vez mais dúctil, a distinção entre princípios e regras adquiriu centralidade. A proposta do artigo é descrever essas mudanças e problematizar os novos dilemas que se apresentam à Teoria do Direito após a constitucionalização: o subjetivismo na decisão judicial e a permeabilidade excessiva do Direito diante da ampliação da complexidade social. Discute-se, ainda, como a Teoria do Direito tem buscado lidar com esses novos desafios – o subjetivismo da decisão tem sido enfrentado por meio de modelos hermenêuticos e modelos de constrangimentos institucionais, já diante da permeabilidade do direito, teóricos como Niklas Luhmann afirmam a necessidade da manutenção de sua autonomia.
A JUDICIALIZAÇÃO DOS TRATAMENTOS CONTRA A DROGADIÇÃO NO ESTADO DO ESPÍRITO SANTO: UMA ANÁLISE ECONÔMICA SOBRE O DISPÊNDIO DOS RECURSOS UTILIZADOS
Authors: Asensi Felipe Dutra , Lopes Luciano Motta Nunes , Monnerat Diego Machado
Published: November 2018

O trabalho objetiva empreender uma análise econômica sobre os gastos que a judicialização dos tratamentos contra a toxicodependência causam nas despesas do Fundo Estadual de Saúde do Espírito Santo (FES). Trata-se de pesquisa documental, exploratória, retrospectiva e descritiva com abordagem quali-quantitativa. Foram utilizados resultados dos estudos sobre os fenômenos da judicialização direta e indireta da saúde, fornecidos pelo Portal da Transparência do Governo do Estado do Espírito Santo e as informações diretamente prestadas pelo FES. Segundo constatou-se em pesquisa realizada pela 1ª Vara da Infância e da Juventude da Comarca de Cachoeiro de Itapemirim/ES, os tratamentos contra a drogadição responderam pela maior parte dos pedidos demandados judicialmente no ano de 2013. Em nível estadual, observou-se que a compra de leitos para tratamentos de dependentes químicos e transtornos mentais, realizadas por força de determinação judicial, responderam por 49,36% do total de pagamentos dessa rubrica no exercício de 2015. Concluiu-se que os problemas relacionados ao uso e abuso de drogas constituem grave problema de saúde pública, ocasionando profundos impactos sociais e econômicos, o que sugere medidas urgentes por parte dos gestores públicos no sentido de se elaborar e implementar políticas de saúde mental eficazes e especificamente direcionadas ao tratamento contra a toxicodependência.
PRIVACIDADE VERSUS SEGURANÇA PÚBLICA: O ACESSO AO CONTEÚDO DE CELULAR PELA AUTORIDADE POLICIAL
Authors: Remedio José Antonio , Silva Bruno Henrique Salviano
Published: November 2018

A pesquisa tem por objeto analisar a relação existente entre o direito à privacidade e o direito à segurança pública, com ênfase à apreensão e ao acesso aos dados de aparelho celular pela autoridade policial, no exercício da persecução penal, sem autorização judicial. A matéria é atual, importante e controvertida, inclusive porque o Supremo Tribunal Federal, na Repercussão Geral no Recurso Extraordinário com Agravo 1.042.075-RJ, em fase de julgamento, reconheceu em 2017 a repercussão geral em relação ao tema referente à perícia realizada pela autoridade policial em aparelho celular encontrado no local do crime, com acesso à agenda telefônica e ao registro de chamadas, sem autorização judicial. O método utilizado é o indutivo, com base na legislação, doutrina e jurisprudência. Tem-se, em conclusão, que o acesso aos dados de aparelho celular apreendido pela autoridade policial no exercício da persecução penal, inclusive no caso de prisão em flagrante do infrator, está condicionado à prévia autorização judicial, embora em casos urgentes ou excepcionais seja admissível o acesso sem anterior autorização, caso em que deverá ser informada a autoridade judicial logo que possível para o exercício do controle do ato, legitimando-se constitucionalmente a restrição exercida em relação ao direito fundamental à privacidade.
PODER POLÍTICO, INSTITUIÇÕES E PLURALISMO JURÍDICO NO MEDIEVO PORTUGUÊS
Authors: Tangerino Davi De Paiva Costa , Olive Henrique
Published: November 2018

O presente trabalho tem como objetivo debater conflitos institucionais em meio a uma experiência histórica de pluralismo jurídico, buscando apresentar um exemplo de disputa por poder político institucional cujo instrumento principal é a expansão jurisdicional. Para isto, aprofunda-se o estudo da clássica historiografia portuguesa sob o crivo dos preceitos metodológicos da historiografia contemporânea, somado a um escrutínio da bibliografia moderna, que nas últimas décadas retomou o debate quanto às relações entre instituições locais e centrais no medievo português, bem como quanto ao exercício de suas jurisdições. Com base nos elementos apresentados, é possível concluir pela importância dos exemplos históricos nos debates sobre instituições políticas e suas interrelações, bem como pelo papel fundamental do Direito enquanto instrumento de expansão e legitimação de poder desde a formação de nossa cultura política.
NOVO PARADIGMA DAS DECISÕES JUDICIAIS NA SOCIEDADE PÓS-MODERNA
Authors: Viana Janile Lima , Sampaio Cunha Danilo Fontenele
Published: November 2018

Analisa as novas relações sociais à luz da Modernidade e da Pós-Modernidade, bem como do positivismo jurídico e pós-positivismo, que, desde o século XX, influenciam a Ciência Jurídica, principalmente no que concerne à interpretação do Direito. É notável a influência, na contemporaneidade, do modelo pós-positivista para justificar as decisões judiciais na busca do justo, utilizando-se, em regra, para a solução de casos mais complexos, conceitos abstratos e princípios implícitos à revelia da lei e da Constituição, desaguando, não raro, no chamado ativismo judicial. Será tratado, primeiramente, sobre a Modernidade e Pós-Modernidade e sua influência no Direito. Em seguida, examinam-se as características dos paradigmas do positivismo e pós-positivismo. E, posteriormente, busca-se compreender o novo paradigma adotado nas decisões judiciais na chamada Pós-Modernidade com crítica ao ativismo judicial. Portanto, é defendida neste experimento acadêmico a ideia de que não houve uma superação do positivismo jurídico pelo pós-positivismo, mas sim um aperfeiçoamento, o qual requer, por parte dos intérpretes, fundamentações consistentes, respeitando as balizas do Estado Democrático de Direito.
Formas antediluvianas da ética em Aristóteles: moral e justiça na Antiguidade Clássica sob uma perspectiva marxista
Authors: Biondi Pablo
Published: November 2018

O artigo concebe a ética como uma forma social profundamente conectada com o capitalismo, tal como o direito, de acordo com a perspectiva de Evgeni Pachukanis. Tal abordagem leva à percepção de que o pensamento moral de Aristóteles não pode ser propriamente considerado como “ético”, pois está baseado na desigualdade da escravidão, e não na igualdade capitalista do sujeito jurídico e ético na modernidade.
Potential and safety tests of egg drop syndrome candidate vaccine from Medan isolate, Indonesia
Authors: Kencana Gusti Ayu Yuniati , Suartha Nyoman , Kardena I Made , Dewi Gusti Ayu Mayani Kristina , Nurhandayani Arini , Syamsidar  , Agustina Kadek Karang
Published: November 2018

Aim: The study was aimed to prepare and examine the potential and safety concerns of egg drop syndrome (EDS) vaccine candidate seed. The potential and safety trials of EDS Medan isolate vaccine need to be done before commercial scale of EDS vaccines are made.

Materials and Methods: The safety test of EDS candidate vaccine was tested on 4-week-old specified pathogen-free chickens in an experimentally isolated enclosure.

Results: The result of the safety test obtained 27.3 hemagglutination inhibition (HI) unit of geometric mean titer antibody post-vaccination. However, the potency test of the EDS candidate vaccine was conducted on 17-week-old laying hens. Test results of the EDS potency vaccine in layer obtained antibody titer increased in every week of blood taking with average titer of antibody: Before vaccinated was 22.9 HI unit, 1 week after vaccination was 23.7 HI unit, 2 weeks post-vaccination was 25 HI unit, and 3 weeks after vaccination was 27.3 HI units. In contrast, decreasing trend was observed in control group (unvaccinated chicken).

Conclusion: Serologically, the seed vaccine of EDS virus isolates from Medan was able to produce protective antibody titers starting in the 2nd and 3rd weeks post-vaccination.
Prevalencia de lactancia materna en menores de 6 meses en el departamento del Huila, Colombia
Authors: Javela Perez Leonel , Javela Rugeles Julian David , Vallejo Cuellar Lisimaco , Rios Gallardo Angela Magnolia , Reyes Gaspar Pedro Leon , Suaza-Calderón Edilberto , Trujillo Bahamon Claudia Leonor , Alarcón Jose Domingo
Published: November 2018

Objetivos: conocer la prevalencia de lactancia materna (exclusiva, predominante y con alimentación complementaria) en bebés <6 meses en el Huila y subregiones, así como factores relacionados.Métodos: estudio con diseño correlacional, descriptivo-transversal, en madres de bebés <6 meses realizado entre octubre-diciembre de 2015. Del total de bebés <6 meses del Huila (N=755), se realizó una encuesta a madres de 335 bebés, incluyendo los 37 municipios, el árearural y urbana. Se realizó muestreo no probabilístico por conveniencia utilizando información del Instituto Colombiano de Bienestar Familiar, centros asistenciales en salud y búsqueda aleatorizada simple para ubicar a las madres.Resultados: en <6 meses se encontró prevalencia de lactancia materna del 95.8 %; lactancia materna exclusiva obtuvo 58.5 %; predominante, 17.6 %; y alimentación complementaria a lactancia materna 19.7%. Categorizando por grupos etarios (meses de 0-1, 2-3 y 4-5), la prevalencia de la lactancia materna exclusiva fue 74.8, 56.4 y 31.3 %, respectivamente. Al sumar la lactancia materna exclusiva con la predominante (lactancia materna completa), las prevalencias fueron 85.3, 72.2 y 63.8 %, respectivamente.Conclusiones: se logró conocer por primera vez para el departamento del Huila las prevalencias en lactancia materna. La prevalencia de lactancia materna, lactancia materna exclusiva, predominante y con alimentación complementaria, tanto en total de <6 meses, categorizando por subgrupos etarios, es mayor que la media referida para Colombia en su Encuesta Nacional de Situación Nutricional (Ensin-2010). Se logró establecer indicadores para subregiones del Huila y caracterización de factores relacionados con lactancia materna.
Halal Meat Supply Chain Traceability based on HACCP, Blockchain and Internet of Things
Authors: Rejeb Abderahman
Published: November 2018

Recently, Halal food has drawn remarkable attention of many consumers around the world. Besides to being unsafe, Halal food such as meat can encounter several issues throughout its supply chain and logistics. At any time, Halal integrity is not guaranteed and risks of becoming non-Halal is the major concern of all parties along the supply chain. To respond to Muslim consumers’ trust concerns in Halal food, many traceability systems were proposed in previous studies based on emerging technologies and  recommended to be incorporated into Halal food supply chains. Nevertheless, all of these systems are centralized, opaque and not enough transparent. To mitigate these problems, blockchain technology is introduced as a ground-breaking innovation with greater decentralization, visibility and transparency. This paper makes a major contribution in suggesting Halal meat supply chain traceability system for real-time food tracing based on embedding Islamic dietary law into HACCP, blockchain and Internet of Things.
Histories of Futures Past: Dystopian Fiction and the Historical Impulse
Authors: Heise-von der Lippe Anya
Published: November 2018

This article traces the historical impulse in two intertextually connected dystopian texts – George Orwell’s1984(1949) and Margaret Atwood’sThe Handmaid’s Tale(1985) – by reading the two novels in the context of the construction of historical narrative after the proclaimed ‘end of history’ in the twentieth century. It considers their representation of history within the framework of literary criticism of the historical novel (György Lukács), critical dystopias (Tom Moylan), and memory as an active, mediated engagement with the past (Astrid Erll and Ann Rigney). It looks, more specifically, at how the texts contrast personal experience and the meta-narrative contemplation of memory with institutionalized versions of history on different diegetic levels by juxtaposing the narrators’/focalizers’ view of history with that presented in the framework of pseudo-historical appendices that accompany and significantly modify the interpretations of both narratives.
Air-Conditioning Comes to the Nation's Capital, 1928–60
Authors: Siry Joseph M.
Published: November 2018

The classical architecture of Washington, D.C., has received much historical attention, but little attention has been paid to the mechanical systems that made these buildings habitable in the capital city's notoriously hot and humid summer climate. In Air-Conditioning Comes to the Nation's Capital, 1928–60, Joseph M. Siry describes how comfort air-conditioning first appeared in the U.S. Capitol building, in the chambers of the House of Representatives and the Senate, in 1928–29. From the 1930s on, air-conditioning systems were extended to the whole of the Capitol, the Senate and House office buildings, the Federal Triangle, and beyond. All of these projects involved the integration of equipment for heating, ventilating, and cooling into the spatial and structural forms of these mostly classical buildings. Air-conditioning had major implications for the annual operations of Congress and the efficiency of federal workplaces. This account advances the historiography of mechanical systems in modern architecture.
Kualitas Sumur Gali di Dusun Wahakaim
Authors: Souisa Gracia Victoria , Y. Janwarin Lea Mediatrix
Published: November 2018

Abstrak

Seluruh kepala keluarga Dusun Wahakaim, Desa Aketernate, memenuhi kebutuhan air bersih dengan mengandalkan sumur gali yang secara fisik tidak memenuhi syarat kualitas air bersih. Penelitian ini bertujuan, mengetahui gambaran kualitas air sumur gali ditinjau dari parameter fisik, kimia dan mikrobiologi sebelum dan sesudah penyaringan sederhana. Desain penelitian yang digunakan adalah survey deskriptif berbasis laboratorium, dengan total sampel sebanyak 12 sampel air. Pemeriksaan kualitas air dilakukan di Laboratorium Kesehatan, Provinsi Maluku, data hasil laboratorium secara deskriptif dibandingkan dengan Permenkes RI Nomor 416/Menkes/Per/IX/1990. Hasil penelitian menunjukan, untuk sampel air yang belum disaring, sebanyak 3 sumur gali (37,5%) tidak memenuhi syarat TDS dan rasa, sebanyak 7 sumur gali (87,5%) tidak memenuhi syarat mangan dan klorida, dan 100% tidak memenuhi syarat Total coli, sedangkan sebanyak 4 sumur gali (50%) tidak memenuhi syarat E.coli dan untuk sampel air yang telah disaring, sebanyak 2 sampel air (50%) tidak memenuhi syarat TDS, sedangkan parameter mikrobiologi sebanyak 3 sampel air (75%) tidak memenuhi syarat Total coli.  Efektifitas penyaringan sederhana optimal pada parameter kekeruhan, TSS  dan mangan.

 

Abstract

All of the family heads of Wahakaim, Aketernate Village, met the need for clean water by relying on dug wells that did not physically meet the requirements of clean water quality. This study aims to find out the description of the water quality of dug wells in terms of physical, chemical and microbiological parameters before and after simple filtering. The research design used was a laboratory-based descriptive survey, with a total sample of 12 water samples. Water quality checks were carried out in the Health Laboratory, Maluku Province, descriptive laboratory data compared to Permenkes RI Number 416 / Menkes / Per / IX / 1990. The results showed that for the water samples that had not been filtered, 3 dug wells (37.5%) did not meet the TDS and taste requirements, as many as 7 dug wells (87.5%) did not meet the requirements of manganese and chloride, and 100% did not meet Total coli requirements, while as many as 4 wells dug (50%) did not meet E.coli requirements and for water samples that were filtered, 2 water samples (50%) did not meet the TDS requirements, while microbiological parameters were 3 water samples (75% ) does not meet the requirements of Total coli. Simple optimal filtering effectiveness on turbidity parameters, TSS and manganese.
Kebiasaan Sarapan pada Mahasiswa Aktif
Authors: Putra Alman , Syafira Dhea Nur , Maulyda Salma , Cahyati Widya Hary
Published: November 2018

Abstrak

Di Provinsi Jawa Tengah terdapat status gizi kurang sebanyak 7,5 %, status gizi normal sebanyak 53,1 %, status gizi gemuk sebanyak 28,1, dan status gizi obes sebanyak 11,2 %. Asupan salah satu faktor yang mempengaruhi status gizi. Asupan salah satunya dapat dipenuhi dengan sarapan. Dalam penelitian ini menggunakan sampel Mahasiswa Semester 1-7 Program Studi Kesehatan Masyarakat Universitas Ngudi Waluyo yang berjumlah 77 orang. Jenis penelitian yang digunakan adalah penelitian yang bersifat deskriptif kualitatif dengan desain penelitian cross sectional. Dari hasil penelitian, dapat diketahui bahwa secara keseluruhan Mahasiswa Pogram Studi Kesehatan Masyarakat memiliki status gizi normal sebanyak 57 responden (74 %). Kebiasaan sarapan terdapat lebih banyak frekuensi sarapan responden 43 (56 %) jarang melakukan sarapan. Lebih banyak 64 responden (83 %) melakukan sarapan sebelum pukul 09.00. Sedangkan responden melakukan sarapan setiap pagi sebanyak 38 (49 %) hampir sama dengan responden melakukan sarapan hanya saat sebelum melakukan aktivitas sebanyak 39 (51 %).

 

Abstract

In Central Java Province, there are less than 7.5% nutrition status, 53.1% normal nutrition status, 28.1 obese nutrition status, and obesity nutrition status of 11.2%. Intake of one of the factors that affect nutritional status. One intake can be filled with breakfast. In this study used a sample of Students Semester 1-7 Ngudi Waluyo Community Health Studies Program which amounted to 77 people. The type of research used is descriptive qualitative research with cross sectional research design. From the results of the research, it can be seen that overall Student Pogram Studies Public Health has normal nutritional status as much as 57 respondents (74%). Breakfast habits are more frequent breakfast 43 respondents (56%) rarely do breakfast. More 64 respondents (83%) did breakfast before 09.00. While respondents do breakfast every morning as much as 38 (49%) almost the same as the respondent to do breakfast just before the activity as much as 39 (51%).
Artocarpus altilis Extracts as Liquid Electric Mosquito Repellent Against Aedes aegypti
Authors: Ernawati Ernawati , Cahyati Widya Hary
Published: November 2018

ABSTRACT

Aedes aegypti is the main vector of dengue virus carriers. One alternative to deal with these problem is by using insecticides derived from the leaves of breadfruit (Artocarous altilis) with the method of liquid electric. The research was purely experimental, with a posttest-only control group design with six variations of extract concentration of 9%, 13%, 20%, 30%, 44%, and 66% with 3 repetitions. The result of Kruskal Wallis test showed that there was a difference of the average number of Aedes aegypti mosquito mortality on various concentration of breadfruit leaf extract, that indicated by significance or probability value of 0.008 <0.05. The deadliest concentration of breadfruit leaf was 66% concentration with mosquito mortality of 83.3% from all mosquito test. The probit analysis obtained LC50 at concentration of 17.850% and LC90 at concentration of 98.348%.

 

Keywords: Aedes aegypti, Breadfruit Leaf Extract, Liquid Electric

 

ABSTRAK

Aedes aegypti merupakan nyamuk yang menjadi vektor utama pembawa virus dengue. Salah satu alternatif lain untuk menangani permasalahan tersebut yaitu dengan menggunakan insektisida yang berasal dari tumbuhan atau  nabati, yaitu daun sukun (Artocarous altilis) dengan metode elektrik cair. Jenis penelitian ini adalah eksperimen murni, dengan rancangan posttest only control group design dengan enam variasi konsentrasi ekstrak sebesar 9%, 13%, 20%, 30%, 44%, dan 66% dengan masing-masing 3 kali pengulangan. Hasil uji Kruskal Wallis menunjukkan bahwa ada perbedaan rata-rata jumlah kematian nyamuk Aedes aegypti pada berbagai konsentrasi ekstrak daun sukun, ditunjukkan dengan nilai signifikansi atau probabilitas adalah 0,008 < 0,05. Konsentrasi ekstrak daun sukun yang paling banyak mematikan nyamuk yaitu konsentrasi 66% dengan kematian nyamuk 83,3 % dari keseluruhan nyamuk. Uji analisis probit didapatkan LC50 pada konsentrasi 17.850% dan LC90 pada konsentrasi 98.348%.

 

Kata kunci: Aedes aegypti, Ekstrak Daun Sukun, Elektrik Cair
Risk Factors of Autism Spectrum Disorder (ASD)
Authors: Hernawan Andri Dwi , Diningrum Aisyah , Jati Sri Nugroho , Nasip M.
Published: November 2018

Abstract

There were 112,000 ASD sufferers in Indonesia in 2012 and in 2015 it was estimated that there were 1 per 250 children or 134,000 sufferers. The proportion of ASD was 62.8% and in 2016 it was 1.28 out of 1000 children in 2015. This study aimed to determine the risk factors for the incidence of ASD in Pontianak City. The research method was analytic observational with a control case study design. The sample was 70 people (35 cases and 35 controls) taken by purposive sampling technique. Data analysis used Chi-Square test (α = 0,05). The results showed that the factors associated with the incidence of ASD were father's age (p = 0.03; OR = 4.00; CI = 1.250-12.804), stress history during pregnancy (p = 0.04; OR = 3.18; CI = 1.13-8.93) and insufficient months of birth (p = 0.036; OR = 4.88; CI = 1.22-19.4), while age of mother during pregnancy, passive smoker, antenatal hemorrhage and pregnancy interval were not associated with the incidence of ASD (p> 0.05). The conclusion of this study is father's age, the presence of a history of stress during pregnancy and insufficient months of birth associated with the incidence of ASD.

Keywords: Father’s age, Stress History, Autism Spectrum Disorder

 

Abstrak

Autism Spectrum Disorder (ASD) dikenal sebagai triad of impairments yaitu adanya gangguan perkembangan pada aspek komunikasi, adanya perilaku yang diulang-ulang dan gangguan dalam berinteraksi dengan lingkungan. Pada tahun 2012 dilaporkan sebanyak 112.000 penderita ASD di Indonesia, tahun 2015 diperkirakan terdapat 1 per 250 anak atau 134.000 penderita. Data dari UPTD Autis Center Kota Pontianak tahun 2015 proporsi ASD sebesar 62,8% dan pada tahun 2016 sebesar 1,28 dari 1000 anak. Penelitian ini bertujuan untuk mengetahui faktor risiko kejadian ASD di Kota Pontianak. Metode penelitian adalah observasional analitik dengan desain studi kasus kontrol.Sampel penelitian berjumlah 70 orang (35 kasus dan 35 kontrol) yang diambil dengan teknik purposive sampling. Analisis data menggunakan uji Chi-Square (α=0,05). Hasil penelitian menunjukkan faktor yang berhubungan dengan kejadian ASD adalah usia ayah (p=0,03; OR=4,00; CI=1,250-12,804), riwayat stres saat hamil (p=0,04; OR=3,18; CI=1,13-8,93) dan lahir belum cukup bulan (p=0,036; OR=4,88; CI=1,22-19,4), sedangkan usia ibu saat hamil, perokok pasif, perdarahan antenatal dan jarak kehamilan tidak berhubungan dengan kejadian ASD (p>0,05). Simpulan penelitian ini adalah faktor usia ayah, adanya riwayat stres saat hamil dan kelahiran belum cukup bulan berhubungan dengan kejadian ASD.

Keyword : Usia ayah, riwayat stress, Autism Spectrum Disorder
Ictiofauna de descarte en la pesca artesanal de camarones (Litopenaeus vannamei y L. stylirostris) en la laguna costera Mar Muerto, Golfo de Tehuantepec, México
Authors: Hernández-Roque Jonny , López-Vila Jesús M. , Romero-Berny Emilio I.
Published: November 2018

Se realizó una caracterización de la ictiofauna de descarte asociada a una pesquería artesanal no regulada de camarón en la laguna costera Mar Muerto, Golfo de Tehuantepec, México. Se realizaron muestreos de las capturas de camarón de octubre 2017 a enero 2018 con redes de copo fijas. En este estudio el descarte integró un porcentaje promedio de 64.2±15.3% de la biomasa total capturada. De este descarte, el grupo de los peces integró entre el 51 y 79.5%, estimándose una proporción general de ictiofauna/camarón de 1.89:1 kg. No se encontró una relación entre la captura de camarón y la biomasa de ictiofauna de descarte. Altos valores de diversidad se presentan en los meses de noviembre y diciembre, debido a una mayor presencia de individuos de afinidad marina. Se demostró que la pesca por copo afecta potencialmente por lo menos a 73 especies de peces, que de acuerdo a su talla media y especie (9.9±9 cm, longitud patrón), pueden corresponder a individuos juveniles y subadultos. Las familias mejor representadas en el elenco íctico fueron Carangidae, Ophichthidae y Gerreidae. Aunque se reporta una riqueza relativamente alta en el descarte, solo 4 especies fueron dominantes. Se destaca la adición de 15 nuevos registros de peces a la ictiofauna conocida en el contexto local y regional. Es necesario realizar caracterizaciones adicionales sobre fauna de acompañamiento y descarte para proponer acciones de manejo y reducción del impacto pesquero.
Renal cell carcinoma: a systematic review and meta-analysis on expression of androgen receptor
Authors: Abdi Hossein , Reza Mozaffari Hamid , Payandeh Mehrdad , Sadeghi Edris
Published: November 2018

Background: Chromosome Xq11-12 is the place that the androgen receptor (AR) sequence appears. Herein, the prevalence of this biomarker and its relation with pT stage and tumor grade was reported.

Methods: Four online sites (PubMed, Scopus, Web of Science, and Cochrane Library) have been searched up to Sep 2018 systematically. Meta-Analysis software version 2.0 (CMA 2.0) and STATA 14.0 statistical software were utilized. Publication bias did not exist.

Results: From the initial 1141 articles identified from the systematically searches. At last, nine of them remained for analysis. The meta-analysis included 1447 patients that 345 of them had AR expression. AR expression significantly correlated with low tumor grade and low tumor pT stage.

Conclusion: AR expression was 28.2%, and it had the relationship with tumor low grade and low pT stage. Additional studies required to figure out the role of it on RCC patients.
Investigating cell blood counter and abdominal radiography accuracy in diagnosis of acute suppurative appendicitis among patients under surgery
Authors: Shahmoradi Mohammad Kazem , Mahmoudvand Hormoz , Nadri Sedigheh , Niazi Massumeh , Adeli Mahin , Rouientan Abdolreza
Published: November 2018

Background: Acute appendicitis is the most common surgical emergencies; while unusual symptoms have a differential diagnosis. This study aimed to determine the diagnostic accuracy of CBC and abdominal X-ray in acute suppurative appendicitis.

Methods: This cross-sectional study was performed on 198 patients. For all patients, complete blood count and abdominal X-ray were recorded. The pathological report after surgery was the gold standard for diagnosis. Then indicators of the validity of tests CBC, X-ray and neutrophil to lymphocyte ratio, consisted of positive predictive value (PPV) and negative predictive value (NPV), sensitivity, specificity, were analyzed.

Results: Out of patients, 133 of patients were male (67.2%), and 65 (32.8%) were female with mean age of 29.13 years. Acute appendicitis confirmed in 77.8% of pathological study, and 17.7% was the normal appendix. Leukocytosis, NLR and abdominal X-ray tests, each have a sensitivity of 89.5%, 78.5% and 100%, specificity of 31.4%, 31.4% and 31.8%, PPV 85.8%, 84.2% and 53.9%, NPV 39.2%, 23.9% and 100%, respectively. There was a significant relationship between appendicitis and WBC> 10.000 and abdominal radiography findings.

Conclusion: Due to the sensitivity of performing CBC and leukocytosis and NLR is at an acceptable level, especially with a relatively high positive predictive value, could be concluded that the positivity of these tests for confirming the diagnosis in suspected cases might be helpful and can help to strengthen the clinical diagnosis. Our results in relation to the x-ray of the abdomen suggest its usefulness in the diagnosis of appendicitis.
Simulation of Rapidly-Exploring Random Trees in Membrane Computing with P-Lingua and Automatic Programming
Authors: Perez-Hurtado Ignacio , Perez-Jumenez Mario , Zhang Gexiang , Orellana-Martin David
Published: November 2018

Methods based on Rapidly-exploring Random Trees (RRTs) have been widely used in robotics to solve motion planning problems. On the other hand, in the membrane computing framework, models based on Enzymatic Numerical P systems (ENPS) have been applied to robot controllers, but today there is a lack of planning algorithms based on membrane computing for robotics. With this motivation, we provide a variant of ENPS called Random Enzymatic Numerical P systems with Proteins and Shared Memory (RENPSM) addressed to implement RRT algorithms and we illustrate it by simulating the bidirectional RRT algorithm. This paper is an extension of [21]a. The software presented in [21] was an ad-hoc simulator, i.e, a tool for simulating computations of one and only one model that has been hard-coded. The main contribution of this paper with respect to [21] is the introduction of a novel solution for membrane computing simulators based on automatic programming. First, we have extended the P-Lingua syntax –a language to define membrane computing models– to write RENPSM models. Second, we have implemented a new parser based on Flex and Bison to read RENPSM models and produce source code in C language for multicore processors with OpenMP. Finally, additional experiments are presented.
Traffic accidents in Spanish road tunnels
Authors: Llopis Serrano Guillermo
Published: November 2018

This paper shows the results of work carried out to study accidents in Spanish road tunnels. The research focuses on tunnels longer than 500 m. It covers 449 accidents in 184 tubes and 230 more accidents close to their portals, all of them with casualties, during a 10-year period. This work analyses several relevant characteristics of traffic accidents in road tunnels, such as their frequency (comparing accident rates in tunnels and on the open road), type and cause. It also estimates the correlation with accident rates involving other characteristics of tunnels, such as circulation (unidirectional as opposed to bidirectional tunnels), length, traffic, longitudinal slope and location. Among other conclusions summarised in this paper, the study shows that tunnels have an accident-reducing effect compared to open stretches of road. This effect increases with tunnel length and it has a major impact for roads with higher accident rates, such as single-carriageway roads.
Varietal and germinative characterization of Agave potatorum (Asparagaceae) seeds with different origins
Authors: Ortiz-Hernández Yolanda Donají , Gutiérrez-Hernández Germán Fernando , Corzo-Ríos Luis Jorge , García-Ramírez Elpidio , Martínez-Tomás Sabino Honorio
Published: November 2018

Background: Agave potatorum reproduces mainly through seeds is widely collected for the mezcal production, and there is currently a marked decrease in their wild populations. Therefore, to contribute to its sustainable use, it is important to characterize the morphology of its seeds and to know their germinative characteristics.Research question: Do the year and the collection site have an effect on the size, color, viability and germination capacity of Agave potatorum seeds?Species studied: Agave potatorum Zucc.Study site and period of research: Seeds of Agave potatorum were collected in Oaxaca, Mexico: Miahuatlán (MIAH), in 2014; Coixtlahuaca (COIX), in 2015; and Zaachila (ZAA), in 2015 and 2016.Methods: For the seeds, we analyzed four physical descriptors, five color coordinates, and nine germination variables. We used a completely randomized design with four replications (100 seeds). An analysis of variance, a comparison of means, and a linear correlation analysis were performed.Results: We found that the ZAA16 seeds showed the highest values ( P < 0.05) for weight and size, and for the luminosity coordinate (L*, 15.2). Color coordinate values revealed a black color of the seeds, which significantly diminished in luminosity with seeds age and varied with their origin. The coordinate L* was correlated significantly with the total dry weight of seedlings and seed volume.Conclusions: Agave potatorum seeds significantly changed their physical dimensions and germination capacity according to their origin. The luminosity of the black color of seeds decreased with seed age and was correlated with seed volume and seedling total dry weight.
Characterization of the secondary metabolites in the seeds of nine native bean varieties ( Phaseolus vulgaris and P. coccineus ) from Querétaro, Mexico
Authors: Quiróz-Sodi Marcela , Mendoza-Díaz Sandra , Hernández-Sandoval Luis , Carrillo-Ángeles Israel
Published: November 2018

Background: Beans ( Phaseolus spp.) are one of the most important legumes due to their high nutritional value. The type and amount of beans’ secondary metabolites varies according to their domestication status, species, and the site where they are grown. In the sate of Querétaro, the most commonly cultivated species are P. vulgaris L. and P. coccineus L., both of which can also be found in wild and disturbed areas.Hypothesis: The aim of this project was to characterize the secondary metabolites in the seeds of the aforementioned species of Phaseolus with different domestication stages and from different geographical areas. The hypothesis is that wild beans collected in Mexican Plateau will have higher concentrations of secondary metabolites.Study site and period of research: The seeds of six samples of domesticated P. vulgaris and three of wild and weedy P. coccineus populations were collected from the Mexican Plateau and the Sierra Madre Oriental in Querétaro, between 2013 and 2016.Methods: It was an experiment with one factor and two levels (bean species) sampled randomized. The experimental unit was each sample, which was studied to obtain 17 chemical parameters, mainly secondary metabolites. From each sample 5 g were selected, minced and parameters were measured twice employing spectrophotometry and high performance liquid chromatography. Data was analyzed using a bootstrap method, discriminant analysis and by establishing simple correlations.Results: The content of secondary metabolites from cultivated P. vulgaris seeds was higher, probably due to its favorable growth environmental and domestication status. Also, beans from the Mexican Plateau had a higher content of secondary metabolites than those from the Sierra Gorda. The analysis of these metabolites allowed for the identification of bean samples with the highest gallic, ellagic, ferulic and p- coumaric acids and vainillin content.Conclusion: The studied samples had different metabolite content according to their species, domestication status and site of growth.
BAM Alignment to CP018802 for Submission to GenBank v1
Authors: P Harhay Gregory
Published: November 2018

Create BAM Alignment FIles including necessary tags that designate library info for submission into the Sequecne Read Archive. Parking the BAM file at the SRA is convenent way to provide alignments to reproduce results at Computational Steps for Analyzing Deep Single Molecule Sequencing Reads of Simple Sequence Repeats - Genomic SSRs
Computational Steps for Analyzing Deep Single Molecule Sequencing Reads of Simple Sequence Repeats - Control SSRs v3
Authors: P Harhay Gregory
Published: November 2018

Simple sequence repeats (SSR) are segments of genome sequence made up of multiple copies of a short sequence (from one to several nucleotides), repeated in series. SSRs are present in prokaryotes(Moxon 2006)and eukaryotes(Toth 2000) however, their biological roles are not fully understood. In some bacterial genomes, SSR length variation has been shown to affect phenotype(van der Woude 2004). SSR length variation occurs through slip-stranded mispairing (SSM) of DNA at a rate of up to a million-fold greater than the average bacterial mutation rate and is therefore termed hypermutable(Moxon 2006). This mode of mutation has been observed in both gram-positive(Danne 2014)and gram-negative bacteria(Inzana 2013)and shown to affect host adaptation, virulence, and pathogenicity. Here we use single-molecule real-time sequencing and a dedicated data processing pipeline to demonstrate that a laboratory culture ofHistophilussomni, prepared from a single, presumably clonal colony, consists of a population ofdistinctsequence phase and read length variant profiles at individual SSR loci. To discriminate between sequencing artifact and biological signal, we employed chemically synthesized control duplexes that we sequenced to create a dataset to tune our computational approach to account for artifacts.Using these control duplexes, we observed low levels of SSM for thePacBio polymerase used in SMRT sequencing.To the best of our knowledge, we present the first protocol for the observation of SSR length variabilityat the single DNA molecule level that accounts for sequencer SSM artifact. Our results demonstrate a method to detect and quantify SSR length heterogeneity in a mixed population of DNA molecules and show that specific SSR regions inHistophilus somniare not static structures well-represented by a single consensus sequence, but rather dynamic structures with length variation differences in multiples of complete repeat unit with characteristic SSM profiles and sequence phase. Immediate applications of this method include direct monitoring of SSR-mediated phase variable gene switching and the development of genome assemblies and annotation that more accurately reflect the sequence diversity of extant genomes. This method may be more broadly applied to study the potentially species-specific interactions of DNA and RNA polymerases and regulatory biomolecules on the one hand, with variations in SSR repeat unit sequence, repeat unit sequence phase, repeat unit length, and hypermutable total length on the other. In this protocol we validate the computaitonal approaches presented here by sequenciing chemically synthesized oligos and annealed to a form duplexes. These oligos are slightly shorter version of the AAGC SSR found in CP018802.1.