bluuebunny/crossref_metadata_2025 huggingface dataset:
Correlations Between pH Values of Oral Fluid and Dental Caries Epidemiologic Indicators in Children Aged within 6-12 Years
Authors: Pop Andreea Simona , Campian Radu Septimiu , Jiman Paula , Ionescu Ecaterina , Milicescu Stefan , Teodorescu Elina , Pacurar Mariana , Bechir Edwin Sever , Mola Farah Curt , Tarmure Viorica
Published: February 2020

The aim of this study was to evaluate the correlations between the pH values of oral fluid and the epidemiologic indicators of dental caries in children aged between 6-12 years. Clinical examinations were performed on 1517 children and the analysis was made in groups of 6-8 years old (n=701) [male (n=353), female (n=348)], and 9-12 years old (n=786) [male (n=426), female (n=360)]. The research included the record the pH values of the oral fluid and clinical examinations for evaluation of the state of oral health, in conformity to the codes of the OMS carious affection level. For the determination and the record of pH values of the oral fluid we used GC Saliva pH Indicator kit. The study followed defs, deft, dmfs, dmft epidemiologic indices of dental caries. The determined values of oral fluid pH were between 5.0 and 6.4. The obtained values of indices in all studied groups and subgroups demonstrated the significant statistical difference between at least two values (p [ 0.001). 72% of the studied group presented with at least a dental surface affected in deciduous teeth, while only 40% of the children presented a carious process on the permanent teeth. The occlusal surface was the most frequently affected by dental caries, followed by the proximal surfaces (mesial and distal) and last being smooth surface caries. The study demonstrated the correlation between acidic values of oral fluid pH and the dental caries in the study group.
Chemical Redistribution and Microstructural Evolution of ODS Fe-Cr Powders During Mechanical Alloying
Authors: Stanciulescu Madalina , Abrudeanu Marioara , Ducu Catalin , Plaiasu Adriana Gabriela
Published: February 2020

The oxide dispersion strengthened ferritic steel powders with chemical composition of Fe-14Cr-3W-0.3Y2O3 were mechanically alloyed from elemental powders in a planetary ball mill. Microstructural and chemical changes at different milling times were investigated by electron microscopy (SEM-EDS) and X-ray diffraction analysis (XRD). It was observed that morphology and structure of powders have experienced many stages during milling, and a quantitative mechanism was proposed. The initiation and evolution of the alloy formation started somewhere around 32 h of mechanical alloying (MA). According to microscopy and XRD analysis, in the first MA stages, milling chiefly has resulted in severe plastic deformation and grain refinement of powders, while in the later stages, alloying was progressed. It seems that 32 hours of milling are necessary to initiate the alloying process of Fe with Cr, but 78 h are not sufficient for completely dissolving W into �-Fe matrix retarding the Fe-Cr-W solid solution formation.
Study of LiFePO4 Electrode Morphology for Li-Ion Battery Performance
Authors: Buga Mihaela , Rizoiu Alexandru , Bubulinca Constantin , Badea Silviu , Balan Mihai , Ciocan Alexandru , Chitu Alin
Published: February 2020

The paper focuses on the development of lithium-ion battery cathode based on lithium iron phosphate (LiFePO4). Li-ion battery cathodes were manufactured using the new Battery R&D Production Line from ROM-EST Centre, the first and only facility in Romania, capable of fabricating the industry standard 18650 lithium-ion cells, customized pouch cells and CR2032 cells. The cathode configuration contains acetylene black (AB), LiFePO4, polyvinylidene fluoride (PVdF) as binder and N-Methyl-2-pyrrolidone (NMP) as solvent. X-ray diffraction measurements and SEM-EDS analysis were conducted to obtain structural and morphological information for the as-prepared electrodes.
The Effect of Some Natural Cytotoxic Peptides on Tumor Cells
Authors: Diaconescu Bogdan Mihail , Jitaru Daniela , Dragos Mihaiela Loredana , Badescu Magda , Stefanache Teodor , Ciocoiu Manuela , Mocanu Madalina , Badescu Codruta
Published: February 2020

Many studies have highlighted the anti-tumor properties of some natural peptides known to have antimicrobial virtues. In this study, we evaluated the tumoricidal potential of dermaseptin, defensin, cecropin A and B on tumor cell lines: M14K (human mesothelioma). The viability study was performed using PE V Annexin and 7-AAD (7-amino-actinomycin D) (BD Pharmingen). This test was used to detect and measure apoptosis by flow cytometry technique. The experimental results of our study revealed that the cytotoxic effects of the four peptides depend on their concentration. In this in vitro experimental study, we found that the cytotoxic effect of the four cytotoxic peptides used depended on their concentration in the tumor cell culture medium, being significant at concentrations of 120mM and maintained at concentrations of 60�M. At 30�M concentrations these tumoricidal effects were insignificant. Of all the studied peptides, dermaseptin has the most powerful effect and the weakest effect b - defensin - 1.
Correlations Between Neuroendocrine Disorders and Biochemical Brain Metabolites Alterations in Antidepressant Treatment
Authors: Ageu Luminita , Talpos Cristina , Kanalas Ghizela , Crisan Simina , Zamfir Carmen Lacramioara , Poroch Vladimir , Anghel Mirela
Published: February 2020

We approach the theme of modern investigation and treatment strategies, based on biochemical, clinical-biological, metabolic, pharmacogenetic, neuro-imagistic, and neuroendocrine integrative correlations in the management of depressive disorders. Our main objective was to investigate: the biochemical brain metabolites [N-acetyl-aspartate (NAA), gamma-aminobutyric acid (GABA), aspartate (Asp), creatine (CR), glutamine (Gln), glicerophosphocholine (GPC), phosphocholine (PC), phosphocreatine (PCr), taurine (Tau), N-methyl-D-aspartate (N-MDA), serine, glycine, choline (Cho)]; the neuroimagistic and neurobiological markers and the metabolic abnormalities in correlation with the molecular pharmacogenetic testing in children and adolescents treated with antidepressant medication. Our research was conducted between 2009-2016 on 90 children and adolescents with depressive disorders -45 children-G1, who benefited of pharmacogenetic testing tailored pharmacotherapy, and 45 without pharmacogenetic testing-G2. The patients were also evaluated by MR spectroscopy at baseline and after pharmacotherapy. The efficacy of the chosen therapy in correlation with the pharmacogenetic testing was evaluated by the mean change in the CDRS (Child Depression Rating Scale) total scores, in the CGI-S/I (Clinical Global Impression Severity/Improvement), CGAS (Clinical Global Assessment of Functioning) and by the change of the relevant neurobiological markers and MR spectroscopy biochemical brain metabolites. Our results showed statistically significant differences in the clinical scores between the studied groups. Our research could represent a proof that the biochemical brain metabolites registered in depressive disorders modified values in the MR spectroscopy and the administration of antidepressants could determine metabolic and neuroendocrine abnormalities (changed lipid profiles, high insulin and plasma glucose levels, weight gain, obesity), especially when chosen without prior pharmacogenetic testing.
Long Term Efficacy of the Treatment with Olanzapine Pamoate, Risperidone and Aripiprazole Monohydrate
Authors: Fodor Ana Aliana , Pascu Alina Mihaela , Poroch Vladimir , Ifteni Petru Iulian , Burtea Victoria , Toma Sebastian , Baracan Adrian , Gavris Claudia , Teodorescu Andreea
Published: February 2020

The use of long-acting injectable antipsychotics (LAIs) is considered to be an important treatment option, especially in early stages of schizophrenia. The aim of this study was to evaluate the efficacy, safety and sustained remission in schizophrenia patients treated with three of the available LAIs substances: olanzapine pamoate, risperidone microspheres and aripiprazole monohydrate. A retrospective chart review study evaluating the efficacy of LAIs compared to oral antispychotics during a five years period was performed. Of the 102 patients included in the study, 52 (50.9%) continued LAIs: olanzapine pamoate (n = 20, 38.4%), risperidone microsphere (n = 22, 42.3%), aripiprazole monohydrate (n = 10, 19.3%). In the LAIs group the number of relapses was smaller than in the oral antipsychotics group (12 vs. 23, P [ 0.05) as well as the number of admissions (15 vs. 30, P [ 0.05). In conclusion, relapse in schizophrenia is strongly related to nonadherence. LAIs prescription overall was underutilized despite their efficacy. Future randomized studies are needed to evaluate the long term efficacy of LAIs compared to oral treatment.
Biochemical Effects of Collagen-Cultured Mesenchymal Stem Cells on Endothelial Progenitor Cells In Vitro
Authors: Cazan Ionut , Susanu Sidonia , Boisteanu Otilia , Tatarciuc Diana , Costan Victor Vlad , Costuleanu Marcel
Published: February 2020

We tempted to explore the biochemical effects, represented by apoptosis, triggered by conditioned medium, obtained as a result of culturing mesenchymal stem cells for 24 h, on endothelial progenitor cells. The mesenchymal stem cells were developed on collagen fibers in the presence of thapsigargin, concanavalin A, leptin and ghrelin. Meanwhile, were used the following inhibitors: AG490, a JAK2 inhibitor; PD98059, a MEK/ERK inhibitor; and LY294002, a PI3K/Akt signaling inhibitor. When we analyzed the results, we observed that the conditioned medium, obtained from mesenchymal stem cells cultivation, applied in a 10% concentration for 24 H , induced apoptosis of endothelial progenitor cells in different degrees: 10 �M leptin] ghrelin]]concanavalin A @ apelin. Furthermore, LY294002 and AG490-conditioned medium of mesenchymal stem cells reduced the apoptotic effects induced by leptin on developed endothelial progenitor cells. The very first conclusion is that the inhibition of PI3K/Akt signaling pathway is blocking the apoptotic effects of leptin stimulation of mesenchymal stem cells population. The inhibition of JAK/STAT pathways seems to be less effective.
Cardiovascular Comorbidities, Inflammation and Serum Albumin Levels in a Group of Hemodialysis Patients
Authors: Balcangiu Stroescu Andra Elena , Tanasescu Maria Daniela , Diaconescu Alexandru , Raducu Laura , Constantin Alexandra Maria , Balan Daniela Gabriela , Tarmure Viorica , Ionescu Dorin
Published: February 2020

Cardiovascular comorbidities have a high rate of prevalence in the chornically hemodialysed patient. The link between these two affections is due to several factors which have a major role in the onset of cardiovascular diseases, such as hypervolemia, nutritional disorders, anemia, hyperuricemia, bone mineral disorders, hyperlipidemia, atherosclerosis. There is also a tight connection between chronic inflammation and cardiovascular diseases in the chronically hemodialysed patient.
Postmenopausal Calcium and Vitamin D Supplements Controversy: Do They Increase Cardiovascular Risk?
Authors: Balanescu Serban , Bojinca Violeta Claudia , Iancu Adrian , Bojinca Mihai , Balanescu Andra
Published: February 2020

Calcium and vitamin D are prescribed in women with osteopenia-osteoporosis to prevent fractures. These bone events increase morbidity and mortality justifying therapy to prevent bone loss. These elderly patients also have cardiovascular risk factors, atherosclerosis and vascular calcifications. Multiple trials demonstrated a relationship between the latter, coronary events and cardiovascular mortality. In these patients, ectopic tissue mineralization may worsen cardiovascular outcome. Calcium intake correlates neither with serum calcium, vascular calcification nor cardiovascular events. However serum calcium-phosphate product is related to vascular calcification and outcome. The main cause for vascular calcification is systemic inflammation and local atherosclerotic process with specific interactions between macrophages and smooth muscle cells. This review examines calcium intake, serum calcium concentration, systemic and vascular inflammation, the mechanisms of vascular calcification and their impact on cardiovascular outcome.
Biochemical Modifications Study of Cerebral Metabolites by Spectroscopy in Epilepsy Treatment
Authors: Nussbaum Laura Alexandra , Hogea Lavinia Maria , Folescu Roxana , Grigoras Mirela Loredana , Zamfir Carmen Lacramioara , Boanca Mihaela , Erdelean Dragos , Rosca Elena Cecilia Ildiko , Nussbaum Liliana , Simu Mihaela Adriana , Lupu Viorel
Published: February 2020

Through this research, our main focus was: to investigate the biochemical brain metabolites (NAA-N-acetylaspartate, GABA-Gama-Aminobutyric Acid, Asp-Aspartate, CR-Creatine, Gln-Glutamine, GPC-Glicerophosphocholine, PC-Phosphocholine, PCr-Phosphocreatine, Tau-Taurine, N-MDA-N-Metyl-D-Aspartate, Serine, Glicine, Cho-Choline); the neuroimagistic, the brain biochemical and metabolic abnormalities in children and adolescents with epilepsy before and after treatment; to review the main antiepileptic medication administered to these patients; and to make some correlations with the results obtained through Magnetic Resonance (MR) Spectroscopy, for further proper early detection and intervention in children and adolescents with epilepsy. Our research was performed between 2010-2017, involving 45 children and adolescents with epilepsy. Also, the patients were evaluated through MR Spectroscopy at baseline and after pharmacotherapy. Through the MR Spectroscopy, we investigated key aspects of the brain function and metabolism. The antiepileptic medication was chosen according to the guides and the type of epileptic seizures. The efficacy of the chosen therapy was evaluated through the change of the relevant MR spectroscopy biochemical brain metabolites. Our results, showed statistically significant modified values and concentrations of the biochemical cerebral metabolites. Our research was a proof, which the evaluation of the biochemical brain metabolites through MR Spectroscopy is of high clinical utility in prevention, early detection and intervention in epilepsy in children and adolescents.
Comparative Study of Heat Treatment Effects Performed with Solar Energy and Electric Furnace on EN 1.4848 Stainless Steel Alloyed with Co, W, Cu and Mo
Authors: Milosan Ioan , Flamant Gilles , Voiculescu Ionelia , Geanta Victor , Munteanu Daniel , Bedo Tibor , Pop Mihai Alin , Semenescu Augustin , Crisan Aurel , Cristea Daniel , Giacomelli Ioan , Stoicanescu Maria , Gabor Camelia , Sarbu Flavius Aurelian , Ghiuta Ioana
Published: February 2020

This paper presents a comparative study of the microstructure characteristics resulted from heat treatments performed with solar energy and with electric resistance furnace of EN 1.4848 steel alloyed with Co-W-Cu-Mo. In order to increase the hardness characteristics, mechanical strength and fatigue, this steel was previously alloyed with 6.15 wt% Co, 1.8 wt% W, 0.3 wt% Cu and 0.2 wt% Mo. The alloying with Co and W aimed at increasing the hardness, while Cu was added to improve the tensile strength and Mo to increase the fatigue strength. The thermal treatment of EN 1.4848 austenitic stainless steel alloyed with Co-W-Cu-Mo consisted in solid solution quenching in liquid, after heating the samples at 1050�C, maintaining the plateau temperature for about 10 min and subsequently cooling in water or oil. The purpose of this treatment was to dissolve the compounds possibly formed during the production of steel, if any, and to form supersaturated solid solutions, stable at low temperatures and in corrosive environments. The microstructural aspects, microhardness, and Differential Scanning Calorimetry (DSC) results were highlighted, in order to emphasize the solid phase transformations, on both heat treatment variants. The microstructure consists of high-alloy austenite, supersaturated with carbon, with small proportions of delta ferrite with interdendritic precipitations and various intermetallic compounds, very stable and without showing phase transformations up to negative temperatures (- 75 C). Comparing the solar quenched samples to the electric-quenched one, regarding to the differential scanning calorimetry (DSC) analysis, showed that independently of the applied cooling process (in water or oil) the phase transformation temperature is lower for the solar-quenched samples compared to the electric-quenched ones.
Heavy Metals (Cr, Cd, Cu, Pb and Zn) Uptake by Cirsium arvense and Agropyron repens
Authors: Alda Simion , Nita Simona , Nita Lucian Dumitru , Rada Maria , Bordean Despina Maria , Alda Liana Maria
Published: February 2020

The purpose of this study was to monitor the heavy metal soil contamination and also to accomplish a comparative study on Cirsium arvense and Agropyron repens heavy metals uptake. The samples were collected from seven different sites situated at various distances from the polluted area Tarnaveni, Romania. Near the chemical wastes spontaneous vegetation is predominant. In this vegetation formed on fallow land, Agropyron repens (monocotyledonate perennial weed) and Cirsium arvense (dicotyledonate perennial weed) are dominant species. The metal (Cr, Cd, Cu, Pb and Zn) concentrations were determined by flame atomic absorption spectrophotometry. The data were statistically analyzed using Main Component Analysis and Generalized Linear Model. Based on our research, we can conclude that Cirsium arvense is accumulating more chromium compared to Agropyron repens and is recommended to be used as an indicator for chromium contamination. The present study highlights that animals consuming spontaneous vegetation grown in this area ingest significant amounts of chromium and zinc.
Experimental and Modelling of Aqueous Radioactive Waste Treatment by Ultrafiltration
Authors: Zicman Laura Ruxandra , Neacsu Elena , Dragolici Felicia Nicoleta , Ciobanu Catalin , Dogaru Gheorghe , Parvulescu Oana Cristina , Dobre Tanase
Published: February 2020

Ultrafiltration of untreated and pretreated aqueous radioactive wastes was conducted using a spiral-wound polysulphonamide membrane. The influence of process factors on its performances was experimental studied and predicted. Permeate volumetric flux and permeate total suspended solids (TSS) were measured at different values of feed flow rate (7 and 10 m3/h), operating pressure (0.1-0.4 MPa), and feed TSS (15 and 60 mg/L). Permeate flux (42-200 L/(m2�h)) increased with feed flow rate and operating pressure as well as it decreased with an increase in feed TSS, whereas permeate TSS (0.1-33.2 mg/L) exhibited an opposite trend. A 23 factorial plan was used to establish correlations between dependent and independent variables of ultrafiltration process.
Preliminary Studies on Citotoxicity and Genotoxicity Assessment of the PMMA-TiO2 Nanocompozites for Stereolithographic Complete Dentures Manufacturing
Authors: Totu Eugenia Eftimie , Cristache Corina Marilena , Isildak Ibrahim , Yildirim Ridvan , Burlibasa Mihai , Nigde Mustafa , Burlibasa Liliana
Published: February 2020

The aim of the present study was to characterize and preliminary assess the cytotoxicity and genotoxicity of different PMMA-TiO2 nanocomposites suitable to be used for two-parts stereolithographic complete denture manufacturing. In order to improve mechanical and antibacterial characteristics of the materials for 3D printing dentures, two matrix materials consisting in commercially available solutions of poly(methyl methacrylate) (PMMA), referred as dent-PMMA respectively base-PMMA, have been doped with different amount of TiO2 nanoparticles: 0.4, 0.6, 1.0, and 4.0% by weight. The obtained nanocomposites were structural characterized and assessed for antibacterial activity, cytotoxicity and genotoxicity. The newly obtained nanocomposites presented a good inhibitory action against the considered bacterial species-Staphylococcus aureus, good biocompatibility under 4.0% by weight. The results of the preliminary studies on cytotoxicity and genotoxicity assessment recommends the use of 0.4% TiO2 dent-PMMA and base-PMMA for CAD/CAM additive manufacturing of two-parts complete dentures/overdentures.
The Use of Pulse Wave Velocity in Predicting Pre-Eclampsia in High-Risk Women
Authors: Ivan Mihaela Viviana , Petre Izabella , Vlaicu Brigitha , Apostol Adrian , Tesloianu Dan , Munteanu Mircea , Costachescu Radmila , Moleriu Lavinia Cristina , Fulger Lazar
Published: February 2020

In this study, we evaluated the diagnostic utility of pulls wave velocity (PWV) alone or in combination with other diagnostic markers in predicting pre-eclampsia (PE) on high-risk women. Pregnant women at high risk for PE were recruited between 32 and 36 weeks of gestation period. This study is the first in Romanian and shows that PWV may be a potentially promising predictor of early-onset PE in women at high risk for PE.
All these patients were treated and were instructed according to the ESC/ESH guidelines regarding hypertensive patients. Statistical analysis was performed using the Chi-square, a Mann-Whitney test, Pearson correlation, and linear regression tests.After we ran several test the main conclusions are that there is a positive correlation between the para-clinics data (creatinine, Esbach proteinuria) and the pulls wave velocity for aorta (PWVao). The risk for preeclampsia is increasing if the body mass index (BMI) of the patients is higher than the average value (BMI]26) or if the patient is a smoker.
Densities, Viscosities and Excess Properties of Binary Mixtures of 2-Methoxy-2-methylpropane with iso-Propanol
Authors: Budeanu Maria Magdalena , Dumitrescu Vasile , Radu Simona , Camenita Alexandru
Published: February 2020

Densities (r) and viscosities (h) of the binary systems 2-Methoxy-2-methylpropane with iso-propanol were measured at temperatures (288.15, 293.15, 298.15, 303.15 and 308.15) K and atmospheric pressure, over the whole composition range. The density and viscosity of the solutions were correlated with the temperature with a linear equation, respectively with Guzman�s equation. The excess values of molar volume (VE), viscosity (hE) and molar Gibbs energy (DG*E) were calculated from experimental measurements. The excess functions of the binary systems were fitted to Redlich-Kister equation. Viscosity results were fitted to the equations of Grunberg-Nissan, Heric-Brewer, Jouyban-Acree and McAllister.
A Ten-year Time Laps, Regarding Drug Consumption in the Western Part of Romania
Authors: Horhat Razvan Mihai , Vlaicu Brigitha , Bagiu Radu , Putnoky Salomeia , Bagiu Iulia , Horhat Delia Ioana , Szuhanek Camelia , Sinescu Cosmin , Negrutiu Meda Lavinia , Nica Luminita
Published: February 2020

The purpose of the research was to see the time-trends (2005/2006-2015/2016) of types and patterns of drug usage between the young adults from medical school in TimisCounty. The study is a comparison of two cross-sectional cohorts of student from Timis County, Romania (2005/2006: 365 females and 248 males; 2005/2016: 336 females and 123 males). In both investigations the same questionnairewas used. Through the investigation we measured the types and patterns of drugs used in relation with gender and cohort effect frequency. For the 2005/2006 evaluation 15% of boys and 4.9% of girls have tried at least one kind of drug. In the reevaluation study 2015/2016 the consumption of at least one kind of drug has risen 34.4% boys and 14.4% girls, which means meaning that the number of consumers has risen by 3 times in between investigations. The intervention to minimize the usage of any sort of drugs in a specific university environment or in the general population has to be done in very careful way in concordance with the specific patterns of usage and types of drugs.
Renal Dysfunction - a Possible Marker of Severity of Heart Failure
Authors: Zaharie Mirela , Carstea Doina , Streba Costin Teodor , Mitrut Paul , Glodeanu Adina Dorina , Carstea Andrei Puiu , Zaharie Sorin Ioan , Dascalu Ionela Teodora , Tuculina Mihaela Jana , Bunget Adina , Bataiosu Marilena , Ionovici Nina
Published: February 2020

Heart failure (HF) and renal dysfunction are frequent associated in the same patient. The purpose of our study was to assess the prevalence of renal dysfunction and the clinical status in admitted patients for decompensated HF. Material and Methods. 397 patients succesively hospitalized for decompensated HF, NYHA III or IV functional class, with left ventricular ejection fraction (LVEF) � 45% were included in the study. Renal dysfunction was defined by glomerular filtration rate (GFR) [ 60 mL/min/1.73 m 2. The mean GFR in patients with HF was 63.89 � 21.5 mL/min/1.73 m2 .The prevalence of renal dysfunction was 49.6%. Patients with GFR [ 60 mL/min/1.73m2, compared with those with preserved renal function were significantly more frequent older (75.37 � 6.84 vs. 71.33 � 8.08 years; p [0.001), females (53,8% vs. 43.5%; p = 0.04), had a significantly higher prevalence of diabetes mellitus (50.2% vs. 28.5%; p [0.001), atrial fibrillation (53.8% vs 46.2%, p = 0.04) and anemia (47.7% vs. 29.5% ; p [0.001). Also, patients with renal dysfunction had more severe HF than those without renal dysfunction (NYHA class IV: 65% vs 45%, p [0.001, clinical congestion: 78.2% vs 68%, p = 0.02, LVEF [35%: 47.21% vs � 35%, p [0.001). Renal dysfunction can be considered an additional marker of severe cardiac dysfunction along with NYHA IV class and low LVEF. The presence of both renal dysfunction and anemia could represent prognostic markers in HF patients with reduced LVEF.
The Assessment of the Effectiveness of Remineralization and Laser Therapy in Cervical Dentine Hypersensitivity
Authors: Ghiorghe Cristina Angela , Dimbu Emilia , Tirca Tiberiu , Andrian Sorin , Topoliceanu Claudiu , Iovan Gianina , Salceanu Mihaela
Published: February 2020

The aim of study was to compare the clinical effectiveness of 810nm laser therapy, remineralising therapy with MI PASTE PLUS� (GC), and toothbrush with Sensodyne� Repair&Protect (GSK) in cervical dentine hypersensitivity. The study was performed on 60 subjects (34 females, 24 males), with at least one tooth affected by cervical dentine hypersensitivity. The patients were randomly divided in three study groups (20 subjects for each group), related to the type of desensitizing therapy: 810nm laser therapy, remineralising therapy with gel MI PASTE PLUS�, toothbrushing with Sensodyne� Repair&Protect). The assessment of the therapy effectiveness was performed by analysis of Schiff Cold Air Sensitivity Scale, with scores directly related to the level of dentine hypersensitivity. For laser group the scores decreased from 2.25 at baseline, at 1.35 at 3 days, and 0.2 at 7 days, with significant statistical differences at 3 days, and 7 days comparing with baseline. For MI PASTE PLUS� (GC) study group the scores decreased from 2.20 at baseline, at 1.60 at 3 days, and 1.30 at 7 days, with significant statistical differences at 3 days, and 7 days comparing with baseline. For Sensodyne� Repair&Protect (GSK) study group the scores decreased from 2.15 at baseline, at 1.90 at 3 days, and 1.45 at 7 days, with significant statistical differences at 3 days, and 7 days comparing with baseline. Low level laser therapy showed better reduction in cervical hypersensitivity, followed by the therapy using MI PASTE PLUS� (GC) gel, and by the toothbrush with Sensodyne� Repair&Protect (GSK) toothpaste.
Chemical Properties of Human Amniotic Membrane for Potential Opthalmological Use
Authors: Costea Claudia Florida , Sadiye Scripcariu Ioana , Dragomir Raluca , Dimitriu Cristina , Turliuc Mihaela Dana , Dumitrescu Gabriela Florenta , Dumitrescu Nicoleta , Vornicu Valeriana , Sava Anca , Cucu Andrei , Turliuc Serban , Carauleanu Alexandru
Published: February 2020

Human amniotic membrane (hAM) allows the exchange of water and organic and inorganic substances between the amniotic fluid, the fetus, and the maternal circulation. Our paper is aimed at determining the chemical composition of the amniotic membrane and at comparing it with the chemical structure of the tears, in order to identify possible uses of the amniotic membrane in eye surgery. In order to determine the chemical composition of hAM we sampled 7 pieces of fresh amniotic membrane, which were processed to create cell homogenate. The chemical tests run on the amniotic membrane specimens in our research revealed the following values: mean glucose concentration = 3mg/100mL and mean total protein concentration = 0.07g/100mL; the electrolyte concentration was: Na+ =152 mEq/L; K+=5.74 mEq/L; Cl- =131.6 mEq/L, and pH=7.2, whereas the total antioxidant capacity (TAC) = 1.1�0.1 mmol/L. These values are similar to those determined by other authors for the amniotic membrane, which resemble those reported for the chemical composition of tear and aqueous humor. To conclude with, the human amniotic membrane is a useful biological material in ophthalmological transplant.
Electrochemical Synthesis of Nanostructured Oxide Layers on Threaded Surfaces of Medical Implants
Authors: Russu Octav Marius , Strnad Gabriela , Jakab-Farkas Laszlo , Cazacu Razvan , Feier Andrei , Gergely Istvan , Trambitas Cristian , Petrovan Cecilia
Published: February 2020

Self organized nanostructured oxide layers were developed on threaded surfaces of medical implants made of Ti6Al4V alloy. The synthesis was done by electrochemical anodization in phosphate/fluoride based electrolyte. By anodization in an aqueous solution of 9.34 wt.% H3PO4 and 0.4 wt.% HF the threaded surfaces were covered by continuous, self ordered nanoporous oxide layers. Scanning electron microscopy (SEM) was used to evaluate the morphology of the nanostructured layers. The diameters of the nanopores depends on anodization potential, by using U = 24 V the openings had an average diameter of 40 nm, while using U = 30V the average nanopores diameter was of 63 nm. The current density was ~10 A/m2 in the steady state of potentiodynamic stage of anodization, and below 45 A/m2 in potentiostatic stage.
Analysis of Internal and Marginal fit of Metal-ceramic Crowns During Processing, Using Conventional and Digitized Technologies
Authors: Savencu Cristina Elena , Porojan Sorin , Porojan Liliana
Published: February 2020

Copings accuracy is an important factor for long-term clinical success of ceramic fused to metal dental restorations. The purpose of the study was to investigate marginal and internal fit of metal-ceramic crowns during processing, using conventional and digitized technologies. Metal copings were produced by Digital light Processing (DLP), invested and casted. Ceramic veneers were overpressed. Internal and marginal fit was measured using silicon replica technique for patterns, casted frameworks and final ceramic fused to metal crowns. Best adaptation was found in resin-pattern group, with small increasing of gap after casting and after ceramic pressing.
Aesthetic and Functional Oral Rehabilitation with Occlussal Trays, Ultrasonotherapy and Tolperison Hydrochloride Treatment in Patients with Dysfunctional Syndrome of Stomatognathic System and Muscular Affectation
Authors: Checherita Laura Elisabeta , Lupu Iulian Costin , Stamatin Ovidiu , Manuc Daniela
Published: February 2020

Our study, a clinic experiment type, was developed on a sample of 77 patients, aged between 60 and 74 years, taken into study and investigated from an electromyographic point of view, which presented the affliction of muscular activity as a result of decreasing or increasing of muscular tonus, or as a result of muscular contraction alterations. We have applied: occlusal trays (28 days) in all of the patients, aerosols therapy in 28 (36.4%) patients and tolperison hydrochloride (50 mg x 3/day, 28 days) in 27 (35.06%) patients. Also, we have applied occlusal trays and tray to be protective with the composite restoration of patients. The effect of augmentation on the local metabolic processes, the analgesia of painful zones and muscular relaxation have positive effects on long and short term. After applications with aerosols, we recorded amelioration in all patients: the absence of pain and the substantial amelioration of pain perception. Conclusions. Physical kinetic therapeutic methods aim at muscular relaxation, the toning up or the decrease of muscular tonus, improvement in mandible functionality, improvement in functionality at the level of temporomandibular joints and improvement in local circulation. The tolperison hydrochloride treatment was used as symptomatic treatment for muscular relaxation with positive results.
Researches Regarding the Improvment of Different Dental Contemporary Composite Materials Structure
Authors: Gradinaru Irina , Ignat Leonard , Doroftei Florica , Ignat Maurusa Elena , Antohe Magda Ecaterina
Published: February 2020

The purpose of this study was to test the structural improving that may be acquired by adding hydroxyapatite microparticles bearing or not silver nanoparticles to three different commercial products commonly used in dental practice. The practical part of this study consisted in the preparation of a common protocol of nine samples resulting from the creation of cavities and their filling similarly to the solving of different clinical situations of carious processes, extracted teeth belonging to both the anterior and the posterior area, with different architectures of the loss of dental substance. Before use, both HA and HA/AgNP have been subjected to calcination in an oven at 6000C for three hours in order to eliminate the residual organic matter. The structural changes have been studied on three types of composites known in the medical practice under the tradenames of Ceram X Duo, Swiss Tec and Herculite XRV Ultra. The structural changes materialized at the level of dentinal component are clearly visible in the SEM images recorded for each starting commercial material. Thus, the addition of HA with high polydispersity (within the range of tens of nanometers and several microns) was effective on lowering the surface rugosity and apparent macro-porosity, while enhancing the phase mixing and component cohesion, but only in the case of Ceram X Duo and Swiss Tec materials. For Herculite XRV Ultra these effects seems to be limited or even reversed, phenomenon that could be explained by the fact that this material already contains nano-fillers, and their concentration tends to saturation.
Chemical Composition and Biological Activity of Birch Bark Extracts on Human and Murine Healthy/Melanoma Cell Lines
Authors: Preda Marius , Cheveresan Adelina , Pinzaru Iulia , Dobrescu Amadeus , Pavel Ioana Zinuca , Vlaia Vicentiu , Sisu Eugen
Published: February 2020

Betulapendula R. presents an increased interest due to its chemical composition, rich in pentacyclictriterpenes, which are recognized for their remarkable biological activityespecially in the pathologies associated with the cutaneous organ. The present research work was purported to assess the biological activity of two type of birch bark extracts, namely total hydro alcoholic extract (BBE1) and betulin purified extract (BBE2) in terms ofviability ontwo healthy cell lines ( human keratinocytes-HaCat and newborn mice epidermis - JB6Cl41-5a) and two melanoma cell lines (human-A375 and murine-B164A5). Both extracts did not exhibited any toxicity on healthy cells at concentrationsd � 25 �g/mL. BBE1 showed greater efficacy than BBE2 on both melanoma cell lines.
TLC, GC-MS, HPLC Analyses and Testing the Antibacterial Effect of Tragopogon pratensis and Vaccinium myrtillus
Authors: Rotaru Luciana Teodora , Istratoaie Octavian , Udrescu Lucretia , Varut Renata Maria , Nicolaescu Oana , Patruna Stefania , Cimpoesu Diana , Corlade Mihaela , Mormoe Mihaela , Florescu Cristina , Nica Silvia , Fortofoiu Maria , Fortofoiu Mircea Catalin
Published: February 2020

Plant extracts with antimicrobial potential are an important research directive in the current medical world, aiming to isolate active components in order to develop new chemotherapeutic agents that can be used in the treatment of various infectious disease. In the present study we determined the content of polyphenol carboxylic acids and flavonoids for two vegetal extracts, by means of TLC, GC-MS and HPLC techniques. Antimicrobial activity was determined on five bacterial species by performing agar diffusion method, using discs impregnated with standard antimicrobial substances and tested plant extracts. Our results showed that the alcoholic extracts Tragoponis pratensis folium and Myrtilli fructus contain polyphenolic compounds and had antimicrobial effect on a wide range of microorganisms (gram positive and negative).
Analysis of Atmospheric Pollutants in Bucharest in Correlation with Meteorological Parameters
Authors: Rusanescu Marin , Rusanescu Carmen Otilia , Paraschiv Gigel
Published: February 2020

In this paper we analyze the correlation between meteorological parameters (wind speed and direction, relative humidity, air temperature) and atmospheric pollutants in Bucharest during the cold period 26.02.2018-02.03.2018, which was based on the monitoring of the concentrations of nitrogen oxides, NO2, O3 and SO2 sulfur dioxide within 24 h and the occurrence of exceedances above the prescribed limit. It was found based on the results obtained that the wind direction influences not only the concentrations of pollutants but also the correlation between the pollutants. Traffic pollutants were at the highest concentration when the wind speed was low. We have found that the highest average concentration for NO2, NOx, NO, O3 occurred at 90% indicative humidity for vertical mixing of strong pollutants. Sulfur dioxide did not record exceeding over the limit standard in the analyzed period.
The Reactor Size Influence on the Optimal Operating Setpoint Choice for a Fixed-Bed Multi-Tubular Catalytic Reactor
Authors: Muscalu Constantin , Maria Gheorghe , Dinculescu Daniel
Published: February 2020

Optimal operation of chemical reactors of high thermal sensitivity is a central engineering problem of very high current interest. One elegant alternative to choose the optimal setpoint when at least two contrary (opposite) objectives are considered is based on the so-called Pareto-optimal front technique. This paper exemplifies how to generate Pareto optimal operating policies when reactor productivity and safety objectives (expressed in probabilistic terms) are simultaneously considered in the presence of technological constraints, uncertainty in safety boundaries, and random fluctuations in control variables. Beside the operating control variables, one important design variable is the reactor pipe diameter because it is directly related to the reaction heat removal. This paper exemplifies the influence of this design variable on the setpoint choice when applying the Pareto-optimal front method with computing the runaway-boundaries by using the generalized sensitivity criterion of Morbidelli and Varma (MV-criterion). An example is provided for an industrial fixed-bed tubular reactor used for the catalytic oxidation of benzene to maleic anhydride (MA) in vapour phase.
The Impact of Residual Bowel After Extended Bowel Resection on Bacterial Overgrowth and Bacterial Translocation
Authors: Osakwe Henry , Nicolescu Cristian , Nicolescu Laura , Hoinoiu Bogdan , Mederle Ovidiu , Mussuto Elisa , Popoiu Calin , Boia Eugen
Published: February 2020

The intestinal mucosa is a major barrier in preventing bacteria invading the body but under certain circumstances mucosal gut barrier can be breached leading to the migration of bacteria to distant organs (mesenteric lymph nodes, spleen and liver). This process is termed bacterial translocation. The abnormal changes in gut ecosystem due to bowel resection led to bacterial overgrowth demonstrated postoperatively in male wistar rats duodenal and stool culture. We proved that after an extended bowel resection there is a direct relation between the number of the intestinal bacteria, the abnormal mucosal structural and functional changes and bacterial translocation to distant organs. A total of 22 male wistar rats divided in 4 groups were studied, short bowel syndrome was simulated in 16 by extended bowel resection (60%, 70% and 75%) while 6 rats had no surgery. Bacterial toxins induced local inflammation which altered neuromuscular tissue function, deteriorated further the gut barrier and increased bacterial overgrowth and bacterial translocation demonstrated by rat�s intestinal biopsy results. The sequence of events leading to translocation remained unclear. The correlation between the percentage of the duodenal aspirate Enterococcus and cecal stool bacteria in rats was R= 0.81, while the correlation between duodenal aspirate Enterococcus and cecal stool bacteria of rats without ileocecal valve resection compared to rats with ileocecal valve resection was R= 0.57 being statistically relevant. Simulated short bowel syndrome by extended intestinal resection caused bacteria overgrowth in residual rat�s bowel and this subsequently led to bacteria translocation to distant organs.
Monitoring the Anthropogenic Toxicity of Spontaneous Flora in Neamt County through Studies of the Honey Bee Chemical Characteristics
Authors: Bodescu Dan , Ungureanu George , Moraru Radu Adrian , Sandu Ioan Gabriel , Bejinariu Costica
Published: February 2020

The products of honey bees can be used as indicators and monitors of a variety of environmental pollutants because of the bees� ability to collect materials that reflect their immediate environmental conditions. Human activities produce more waste and administrate the pesticides, the amounts and toxicity of which often exceed the environment�s homeostatic capacity to cleanse itself, and this is constantly transforming due to intensive agricultural practices necessary to increase food production as human population grows. The main sources for contamination of honey with heavy metals are represented by placing hives near urban areas with heavy car traffic, or industrialized areas and the use on the entire circuit of production, objects or containers made of materials unsuitable (unacceptable). For that reason regular monitoring of the environment is so important. Honey bees, thanks to their morphological features, and also bee products are regarded as good indicators of environmental pollution by toxic substances, be these heavy metals, radioactive elements or persistent organic pollutants such as pesticides. Consequently, it is important to estimate the environmental fate and Eco toxicological effects of these different xenobiotic. Honey bees (Apis mellifera L.) have been used as biological indicators of Plant Protection Products (PPPs) in two intensely cultivated in areas of Neamt County, Romania. This area is representative for the pre-mountain and mountain zone of Romania. The stratified sample has been face-to-face interviewed in 2016 regarding the data from the year 2015. The total consumption specific for the honey production was about 628 MJ hive-1, and the energy output reached 235 MJ hive-1, determining an energy productivity of 0.030 kg MJ-1 and an energy use efficiency of 0.37. Specific energy amounted 33.3MJ kg-1 due to the inefficiency of traveling during the apiaries movements and the inappropriate correlation between the apiaries size and the zonal melliferous potential. In this paper available literature data and information on the morphological features of the honey bee, the utilization of the honey bee and its products as indicators of environmental pollution, and a historical outline of some of the legislation relating to beekeeping have been critically compared and discussed.
Release of Polyphenols from Liposomes Loaded with Echinacea purpurea
Authors: Pavaloiu Ramona Daniela , At Fawzia Sha , Bubueanu Corina , Neagu Georgeta , Albulescu Adrian , Hlevca Cristina , Nechifor Gheorghe
Published: February 2020

The aim of this study was to develop a delivery system for polyphenols from an extract of Echinacea purpurea leaves, based on liposomes. Liposomes loaded with Echinacea purpurea were prepared and characterized in terms of entrapment efficiency, size, polydispersity index, stability and release behavior. Results showed good entrapment efficiency, small sizes, low polydispersity index and good stability over 90 days at 4oC. Also, the liposomal formulations presented reduced burst release and slow release of polyphenols compared with free extract. Therefore, liposomes offer a great potential in the development of drug delivery systems for polyphenols.
Functional Tannins in Grape Pomace Flours of Feteasca Neagra and Italian Riesling
Authors: Muncaciu Maria Laura , Zamora Marin Fernando , Pop Nastasia , Babes Anca Cristina , Bunea Claudiu Ioan , Balea Stefania Silvia , Calugar Anamaria
Published: February 2020

The aim of this study was to analyze the content of functional tannins in grape pomace skin, seed, and mixed flour, from Feteasca Neagra and Italian Riesling varieties. Results showed that all compounds were present in the highest concentration in grape pomace seed flours and the lowest in skin flours. The concentration of total tannins and total flavan-3-ol monomers in all flour samples ranged from 37.34 to 72.28 mg epicatechin/g dry weight and 0.57 to 3.13 mg/g dry weight, respectively. Among monomers, (+)-catechin was found in the highest concentration, except in Italian Riesling seed flours, where (-)-epicatechin was predominant. The mDP of proanthocyanidins in seed flour (7.15 to 8.07) is lower than that of skin flours (10.83 to 18.43) while the opposite was observed for %G. As a result, all Feteasca Neagra and Italian Riesling grape pomace flours, especially seed flour, could be used as sources of functional tannins in alimentary or pharmaceutical industry.
Evaluation of Biochemical and Clinical Parameters of Hypertension with Type 2 Diabetes Mellitus
Authors: Nicola Patricia , Ardeleanu Elena , Gadau Carmen , Dorobantu Maria , Darabont Roxana , Tilea Ioan , Varga Andreea , Folescu Roxana , Zamfir Alexandra Simona , Boanca Mihaela , Strat Liliana , Baaj Teim , Gurgus Daniela
Published: February 2020

The aim of this study was to assess the basic biochemical and clinical characteristics of patients with hypertension and type 2 diabetes mellitus (T2DM), office blood pressure (BP) and 24-h BP profile, their risk factors and associated comorbidities. Compared with non-diabetics, hypertensive patients with T2DM were older, with a longer duration of hypertension (5.9 vs. 4.7 years), greater office blood pressure and ambulatory BP values, increased incidence of multiple risk factors, target organ damage and cardiovascular disease. Biochemical data in hypertension with T2DM revealed significantly high levels of LDL cholesterol, triglycerides, creatinine, micro- and macro-albuminuria and a reduced estimated glomerular filtration rate. The presence of diabetes was associated with obesity, represented by a BMI ]30 kg/m2 (OR 2.08 [95% CI 1.26-3.45], p = 0.004), abdominal obesity (OR 1.85 [95% CI 1.11-3.04], p = 0.016), high LDL cholesterol (OR 2.02 [95% CI 1.22-3.35], p = 0.006) and high triglycerides (OR 1.86 [95% CI 1.11-3.11], p = 0.017).
Performance of MFI Zeolite Catalysts in n-heptane Conversion Reaction Test
Authors: Asaftei Iuliean Vasile , Lungu Neculai Catalin , Sandu Ioan Gabriel , Spac Adrian Florin , Ignat Maria
Published: February 2020

The aromatization of n-heptane, one of the main components of light naphtha, has been investigated over HZSM-5 (Si/Al = 33.9) and Zn-HZSM-5: Zn1-HZSM-5 (0.86% Zn); Zn2-HZSM-5 (1.35% Zn) and Zn3-HZSM-5 (2.89% Zn) catalysts prepared by the ion exchange with 10M aqueous solution of Zn(NO3)2. The catalytic activity measurement was performed by the chromatographic pulse method in the temperature range of 673 - 823K. The presence of zinc in Zn-HZSM-5 catalysts provided a significant increase in total aromatics selectivity due to the improvement of dehydrogenation activity by Zn incorporation. The Zn species facilitated the aromatization by converting paraffin�s into corresponding olefins which, in turn, were the aromatics precursors. Therefore, both acidity and zinc incorporation played important roles in the aromatization of n-heptane. Over 773 K the activity and selectivity of the Zn-HZSM-5 catalysts decrease very probably due to the loss of zinc ions.