bluuebunny/crossref_metadata_2025 huggingface dataset:
Context association in pyramidal neurons through local synaptic plasticity in apical dendrites
Authors: Baronig Maximilian , Legenstein Robert
Published: January 2024

The unique characteristics of neocortical pyramidal neurons are thought to be crucial for many aspects of information processing and learning in the brain. Experimental data suggests that their segregation into two distinct compartments, the basal dendrites close to the soma and the apical dendrites branching out from the thick apical dendritic tuft, plays an essential role in cortical organization. A recent hypothesis states that layer 5 pyramidal cells associate top-down contextual information arriving at their apical tuft with features of the sensory input that predominantly arrives at their basal dendrites. It has however remained unclear whether such context association could be established by synaptic plasticity processes. In this work, we formalize the objective of such context association learning through a mathematical loss function and derive a plasticity rule for apical synapses that optimizes this loss. The resulting plasticity rule utilizes information that is available either locally at the synapse, through branch-local NMDA spikes, or through global Ca2+events, both of which have been observed experimentally in layer 5 pyramidal cells. We show in computer simulations that the plasticity rule enables pyramidal cells to associate top-down contextual input patterns with high somatic activity. Furthermore, it enables networks of pyramidal neuron models to perform context-dependent tasks and enables continual learning by allocating new dendritic branches to novel contexts.
EEG-based high-performance depression state recognition
Authors: Wang Zhuozheng , Hu Chenyang , Liu Wei , Zhou Xiaofan , Zhao Xixi
Published: January 2024

Depression is a global disease that is harmful to people. Traditional identification methods based on various scales are not objective and accurate enough. Electroencephalogram (EEG) contains abundant physiological information, which makes it a new research direction to identify depression state. However, most EEG-based algorithms only extract the original EEG features and ignore the complex spatiotemporal information interactions, which will reduce performance. Thus, a more accurate and objective method for depression identification is urgently needed. In this work, we propose a novel depression identification model: W-GCN-GRU. In our proposed method, we censored six sensitive features based on Spearman’s rank correlation coefficient and assigned different weight coefficients to each sensitive feature by AUC for the weighted fusion of sensitive features. In particular, we use the GCN and GRU cascade networks based on weighted sensitive features as depression recognition models. For the GCN, we creatively took the brain function network based on the correlation coefficient matrix as the adjacency matrix input and the weighted fused sensitive features were used as the node feature matrix input. Our proposed model performed well on our self-collected dataset and the MODMA datasets with a accuracy of 94.72%, outperforming other methods. Our findings showed that feature dimensionality reduction, weighted fusion, and EEG spatial information all had great effects on depression recognition.
Can pluripotent/multipotent stem cells reverse Parkinson’s disease progression?
Authors: Wu Yongkang , Meng Xiangtian , Cheng Wai-Yin , Yan Zhichao , Li Keqin , Wang Jian , Jiang Tianfang , Zhou Fei , Wong Ka-Hing , Zhong Chunlong , Dong Yi , Gao Shane
Published: January 2024

Parkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by continuous and selective degeneration or death of dopamine neurons in the midbrain, leading to dysfunction of the nigrostriatal neural circuits. Current clinical treatments for PD include drug treatment and surgery, which provide short-term relief of symptoms but are associated with many side effects and cannot reverse the progression of PD. Pluripotent/multipotent stem cells possess a self-renewal capacity and the potential to differentiate into dopaminergic neurons. Transplantation of pluripotent/multipotent stem cells or dopaminergic neurons derived from these cells is a promising strategy for the complete repair of damaged neural circuits in PD. This article reviews and summarizes the current preclinical/clinical treatments for PD, their efficacies, and the advantages/disadvantages of various stem cells, including pluripotent and multipotent stem cells, to provide a detailed overview of how these cells can be applied in the treatment of PD, as well as the challenges and bottlenecks that need to be overcome in future translational studies.
The domestic chick as an animal model of autism spectrum disorder: building adaptive social perceptions through prenatally formed predispositions
Authors: Matsushima Toshiya , Izumi Takeshi , Vallortigara Giorgio
Published: January 2024

Equipped with an early social predisposition immediately post-birth, humans typically form associations with mothers and other family members through exposure learning, canalized by a prenatally formed predisposition of visual preference to biological motion, face configuration, and other cues of animacy. If impaired, reduced preferences can lead to social interaction impairments such as autism spectrum disorder (ASD) via misguided canalization. Despite being taxonomically distant, domestic chicks could also follow a homologous developmental trajectory toward adaptive socialization through imprinting, which is guided via predisposed preferences similar to those of humans, thereby suggesting that chicks are a valid animal model of ASD. In addition to the phenotypic similarities in predisposition with human newborns, accumulating evidence on the responsible molecular mechanisms suggests the construct validity of the chick model. Considering the recent progress in the evo-devo studies in vertebrates, we reviewed the advantages and limitations of the chick model of developmental mental diseases in humans.
Fecal supernatants from dogs with idiopathic epilepsy activate enteric neurons
Authors: Elfers Kristin , Watanangura Antja , Hoffmann Pascal , Suchodolski Jan S. , Khattab Mohammad R. , Pilla Rachel , Meller Sebastian , Volk Holger A. , Mazzuoli-Weber Gemma
Published: January 2024

Introduction

Alterations in the composition and function of the gut microbiome have been reported in idiopathic epilepsy (IE), however, interactions of gut microbes with the enteric nervous system (ENS) in this context require further study. This pilot study examined how gastrointestinal microbiota (GIM), their metabolites, and nutrients contained in intestinal contents communicate with the ENS.

Methods

Fecal supernatants (FS) from healthy dogs and dogs with IE, including drug-naïve, phenobarbital (PB) responsive, and PB non-responsive dogs, were applied to cultured myenteric neurons to test their activation using voltage-sensitive dye neuroimaging. Additionally, the concentrations of short-chain fatty acids (SCFAs) in the FS were quantified.

Results

Our findings indicate that FS from all examined groups elicited neuronal activation. Notably, FS from PB non-responsive dogs with IE induced action potential discharge in a higher proportion of enteric neurons compared to healthy controls, which exhibited the lowest burst frequency overall. Furthermore, the highest burst frequency in enteric neurons was observed upon exposure to FS from drug-naïve dogs with IE. This frequency was significantly higher compared to that observed in PB non-responsive dogs with IE and showed a tendency to surpass that of healthy controls.

Discussion

Although observed disparities in SCFA concentrations across the various FS samples might be associated with the induced neuronal activity, a direct correlation remains elusive at this point. The obtained results hint at an involvement of the ENS in canine IE and set the basis for future studies.
Primary gastrinoma of the gallbladder: a case report and review of the literature
Authors: Liu Yao-Ge , Jiang Shi-Tao , Zhou Yang , Zhang Jun-Wei , Sang Xin-Ting , Zhang Lei , Lu Xin , Xu Yi-Yao
Published: January 2024

Background

Primary gallbladder gastrinoma is an exceptionally uncommon tumor and is a rare form of neuroendocrine neoplasm. Until now, no cases of primary gallbladder gastrinoma and rare cases of primary gastrinoma from the biliary system have been reported.

Case presentation

We report a case of a 50-year-old woman with watery diarrhea who intermittently received proton pump inhibitors (PPIs) as treatment. A serum gastrin level of 711 pg/ml was recorded after the withdrawal of PPI over 1 week. Enhanced computed tomography (CT) imaging and octreotide imaging uncovered a solitary tumor at the hepatic hilar region. During the laparoscopic surgery, it was determined that the tumor had its origin in the wall of the gallbladder neck, prompting the implementation of a laparoscopic cholecystectomy. Histological analysis revealed a primary neuroendocrine tumor from the neck of the gallbladder. The patient’s symptoms disappeared after the surgery with a follow-up of 6 months.

Conclusions

This case confirmed that primary gallbladder gastrinoma represents a distinct nosological entity. Immunohistochemical analysis plays a pivotal role in the diagnostic process. Given the limited understanding of primary gallbladder gastrinoma, our objective is to offer novel insights into this rare disease by delivering distinctive information and highlighting the therapeutic significance of surgical intervention.
Adenovirus and Mycoplasma pneumoniae co-infection as a risk factor for severe community-acquired pneumonia in children
Authors: Chen Qihong , Lin Lihua , Zhang Ning , Yang Yungang
Published: January 2024

Background

To investigate the pathogenic characteristics and risk factors of pediatric severe community-acquired pneumonia (CAP).

Methods

We retrospectively analyzed the clinical data of hospitalized children with severe CAP from April 2014 to June 2019 in China. Data of age, sex and pathogenic results were collected: bacterial and fungal cultures, respiratory viruses from sputum or bronchoalveolar lavage fluid (BALF), serum Mycoplasma pneumoniae (MP)-IgM and Chlamydia Pneumoniae-IgM, and BALF or blood (1-3)-β-D-glucan/galactomannan test.

Results

A total of 679 children with severe CAP were included in the analysis. The number of cases infected with MP was higher in males than in females. There were significant differences between the ≤1-year and >1-year groups in terms of pathogen. The top three bacteria cultured were Haemophilus influenzae (57/679, 8.4%), Streptococcus pneumoniae (50/679, 7.4%), and Pseudomonas aeruginosa (25/679, 3.7%). The top three viruses detected were adenovirus (AdV, 124/679, 18.3%), respiratory syncytial virus (24/679, 3.5%), and parainfluenza virus (21/679, 3.1%). AdV and MP were the leading pathogens, detected in 18.3% and 32.6% cases, respectively. MP infection increased the risk of AdV infection (OR 3.77, p < 0.0001). MP infection was a risk factor for severe AdV-infected pneumonia, while sex, age, bacteria, Chlamydia Pneumoniae, fungal, and AdV infections were risk factors for severe MP-infected pneumonia.

Conclusions

AdV and MP were dominant pathogens in children with severe CAP. AdV and MP infection predisposed each other to develop severe illness. AdV-MP co-infection may lead to severe pneumonia.
Tolerability, safety, and pharmacokinetics of a single intravenous administration of a novel recombinant humanized anti-interleukin-6 receptor monoclonal antibody in healthy Chinese volunteers
Authors: Jiang Xin , Lin Pingping , Sun Feifei , Xu Yi , Tao Ye , Shi Ping , Liu Yanping , Li Xin , Liu Shuqin , Gao Xiaomeng , Wang Chenjing , Cao Yu
Published: January 2024

Aim: VDJ001 is a novel recombinant humanized monoclonal antibody against the anti-interleukin-6 receptor. As an analog of tocilizumab, it exhibited improved affinity and in vitro activity. Based on preclinical studies, a first-in-human clinical study was conducted to evaluate the safety, tolerability, and pharmacokinetics of VDJ001.

Methods: This is a single-center, randomized, double-blinded, placebo-controlled phase I dose-escalation study conducted in healthy Chinese volunteers. Four cohorts were designed with dosages ranging from 1 to 8 mg/kg. There were equal numbers of female and male volunteers in each cohort. Enrolled subjects randomly received a single intravenous administration of VDJ001 or placebo (VDJ001: placebo = 4:1 in both female and male volunteers). Three sentinel volunteers in the 1 mg/kg cohort were first administered, and the treatment of the other seven volunteers was carried out after a safety assessment on D15. The following cohort was conducted only when the safety profile was evaluated as acceptable on D29 of the previous cohort. Samples for pharmacokinetics (PK), pharmacodynamics (PD), and immunogenicity were collected at specified time points and analyzed through validated methods. Adverse events and the results of the examination and laboratory were analyzed to assess the safety profile.

Results: All cohorts were carried out according to the protocol. With the escalation of dosage, Cmax increased linearly, and AUC0-t and AUC0-∞ increased in a non-linear manner, while clearance decreased and t1/2 prolonged. Six volunteers who received VDJ001 tested ADA-positive, among whom one participant tested Nab-positive on D57. One volunteer in the placebo group tested ADA-positive but Nab-negative. CRP concentrations were not found to be correlated with the dosage. Both IL-6 and sIL-6R concentrations increased after the administration of VDJ001. All adverse events were mild to moderate in severity. No serious adverse events were reported in this study. No unexpected or clinically significant safety issues were found.

Conclusion: The safety and tolerability of VDJ001 are acceptable with a single intravenous dosage of 1∼8 mg/kg. Further clinical trials are warranted.
Pulmonary nitric oxide in astronauts before and during long-term spaceflight
Authors: Karlsson Lars L. , Gustafsson Lars E. , Linnarsson Dag
Published: January 2024

Introduction: During exploratory space flights astronauts risk exposure to toxic planetary dust. Exhaled nitric oxide partial pressure (PENO) is a simple method to monitor lung health by detecting airway inflammation after dust inhalation. The turnover of NO in the lungs is dependent on several factors which will be altered during planetary exploration such as gravity (G) and gas density. To investigate the impacts of these factors on normal PENO, we took measurements before and during a stay at the International Space Station, at both normal and reduced atmospheric pressures. We expected stable PENO levels during the preflight and inflight periods, with lower values inflight. With reduced pressure we expected no net changes of PENO.

Material and methods: Ten astronauts were studied during the pre-flight (1 G) and inflight (µG) periods at normal pressure [1.0 ata (atmospheres absolute)], with six of them also monitored at reduced (0.7 ata) pressure and gas density. The average observation period was from 191 days before launch until 105 days after launch. PENO was measured together with estimates of alveolar NO and the airway contribution to the exhaled NO flux.

Results: The levels of PENO at 50 mL/s (PENO50) were not stable during the preflight and inflight periods respectively but decreased with time (p = 0.0284) at a rate of 0.55 (0.24) [mean (SD)] mPa per 180 days throughout the observation period, so that there was a significant difference (p < 0.01, N = 10) between gravity conditions. Thus, PENO50 averaged 2.28 (0.70) mPa at 1 G and 1.65 (0.51) mPa during µG (−27%). Reduced atmospheric pressure had no net impact on PENO50 but increased the airway contribution to exhaled NO.

Discussion: The time courses of PENO50 suggest an initial airway inflammation, which gradually subsided. Our previous hypothesis of an increased uptake of NO to the blood by means of an expanded gas-blood interface in µG leading to decreased PENO50 is neither supported nor contradicted by the present findings. Baseline PENO50 values for lung health monitoring in astronauts should be obtained not only on ground but also during the relevant gravity conditions and before the possibility of inhaling toxic planetary dust.
Sense of agency in joint action: a critical review of we-agency
Authors: Le Besnerais Alexis , Moore James W. , Berberian Bruno , Grynszpan Ouriel
Published: January 2024

The sense of agency refers to the experience of control over voluntary actions and their effects. There is growing interest in the notion of we-agency, whereby individual sense of agency is supplanted by a collective agentic experience. The existence of this unique agentic state would have profound implications for human responsibility, and, as such, warrants further scrutiny. In this paper, we review the concept of we-agency and examine whether evidence supports it. We argue that this concept entails multiplying hypothetical agentic states associated with joint action, thus ending up with an entangled phenomenology that appears somewhat speculative when weighted against the available evidence. In light of this, we suggest that the concept of we-agency should be abandoned in favor of a more parsimonious framework for the sense of agency in joint action.
Trust in leadership and perceptions of justice in fostering employee commitment
Authors: González-Cánovas Alejandro , Trillo Alejandra , Bretones Francisco D. , Fernández-Millán Juan M.
Published: January 2024

In a period of uncertainty, trust in leadership and perceptions of fairness have emerged as pivotal factors for fostering employee identification and affective commitment. Drawing from authentic leadership theory, this leadership style is identified as a crucial antecedent of affective commitment, examining the mediating role of distributive justice and the moderating role of interactional justice. A quantitative approach was employed, utilizing data from 302 questionnaires completed by Spanish retail workers. For data analysis, SPSS v.25 was used to generate descriptive statistics, while partial least squares structural equation modeling was applied to test the proposed hypotheses. Our findings revealed that authentic leadership is positively associated with the development of affective commitment, with distributive justice acting as a mediating factor between the two. Furthermore, interactional justice negatively moderates the relationship between distributive justice and affective commitment. Contrary to initial expectations, the second moderation, between authentic leadership and affective commitment, was not found to be significant. The research concludes by discussing the practical implications of the results.
Distribution and potential risk factors of bisphenol a in serum and urine among Chinese from 2004 to 2019
Authors: Zhang Wenjing , Li Yanting , Wang Tao , Zhang Xinglin , Zhang Jianzhong , Ji Xiaoya , Lu Lin
Published: January 2024

Background

Bisphenol A (BPA) is an oil-derived, large-market volume chemical with endocrine disrupting properties and reproductive toxicity. Moreover, BPA is frequently used in food contact materials, has been extensively researched recently, and widespread exposure in the general population has been reported worldwide. However, national information on BPA levels in general Chinese people is lacking.

Methods

This study collected and analyzed 145 (104 in urine and 41 in serum) research articles published between 2004 and 2021 to reflect the BPA internal exposure levels in Chinese populations. The Monte Carlo simulation method is employed to analyze and estimate the data in order to rectify the deviation caused by a skewed distribution.

Results

Data on BPA concentrations in urine and serum were collected from 2006 to 2019 and 2004 to 2019, respectively. Urinary BPA concentrations did not vary significantly until 2017, with the highest concentration occurring from 2018 to 2019 (2.90 ng/mL). The serum BPA concentration decreased to the nadir of 1.07 ng/mL in 2011 and gradually increased to 2.54 ng/mL. Nationally, 18 provinces were studied, with Guangdong (3.50 ng/mL), Zhejiang (2.57 ng/mL), and Fujian (2.15 ng/mL) having the highest urine BPA levels. Serum BPA was investigated in 15 provinces; Jiangsu (9.14 ng/mL) and Shandong (5.80 ng/mL) were relatively high. The results also indicated that males’ urine and serum BPA levels were higher than females, while the BPA levels in children were also higher than in adults (p < 0.001). Furthermore, the volume of garbage disposal (r = 0.39, p < 0.05), household sewage (r = 0.34, p < 0.05), and waste incineration content (r = 0.35, p < 0.05) exhibited a strong positive connection with urine BPA levels in Chinese individuals.

Conclusion

Despite using a data consolidation approach, our study found that the Chinese population was exposed to significant amounts of BPA, and males having a higher level than females. Besides, the levels of BPA exposure are influenced by the volume of garbage disposal, household sewage, and waste incineration content.
Assessing the impact of COVID-19 on epidemiological changes of severe pediatric respiratory syncytial virus infections in Malaysia
Authors: Chan Chee Mun , Wahab Asrul Abdul , Ali Adli
Published: January 2024

Introduction

Respiratory syncytial virus (RSV) is one of the leading causes of hospitalization and mortality among children with respiratory tract infections. The non-pharmaceutical preventive measures against severe acute respiratory syndrome coronavirus (COVID-19) may have reduced the transmission of RSV, altering its tropical epidemiological seasonality. Thus, this study represents the first attempt to evaluate changes in RSV epidemiology in the context of COVID-19 pandemic in Malaysia.

Methods

Conducted at a tertiary hospital in Kuala Lumpur, Malaysia, this retrospective study analyzed collated data of children aged <12 years who were admitted for severe respiratory infections from 2017 to 2022. Time series models were used to predict the differences between actual and forecasted RSV cases, while logistic regression assessed the statistical association between RSV and COVID-19.

Results

Among the 4,084 children analyzed, we reported a significant inverse relationship between RSV and COVID-19 infections during the pandemic (2020–2021) (p < 0.05). In 2020, the RSV positivity rate sharply declined to 8.3 and 5.9%, respectively, in the two prominent seasons. Time series analysis showed a tremendous decrease in cases compared to the expected values, with reductions of 98.3% in the first season and 95.7% in the second season. However, following the lifting of the restriction order in 2022, RSV infections rose sharply with a positivity rate of 36.3%, higher than pre-COVID-19 pandemic levels.

Conclusion

This study provides evidence of increasing RSV cases post-COVID-19 pandemic, due to immunity debt. Hence, the healthcare system must be prepared to address future RSV outbreaks with the appropriate implementation of prophylaxis and public health measures.
The Importance of Sea Ice to Antarctic Ice Shelves
Authors: Miles Bertie W. J. , Arthur Jennifer F. , Gomez-Fell Rodrigo
Published: January 2024

Ice shelves are important because they act as a dam that regulates the amount of land ice that flows into the ocean. This means if Antarctica’s ice shelves were to break up, more ice would be melted into the ocean and global sea level would rise at a much faster rate. Because Antarctic ice shelves float in the ocean, they are not only sensitive to warming air temperatures but also to changes in the Southern Ocean. One such important factor is how ice shelves interact with sea ice. When sea ice attaches to the front of ice shelves, it can act as a glue, preventing ice-shelf break-up and promoting ice-shelf growth. In the open ocean, sea ice can also help protect ice shelves from damaging ocean waves formed during large storms. Sea ice will play an important role in determining the fate of Antarctic ice shelves in the coming decades.
Who Can Use Your Cells Or Tissues For Scientific Research?
Authors: Beers Jessica L. , Geffert Raeanne M. , Latham Bethany D. , Jackson Klarissa D.
Published: January 2024

Informed consent is the process of obtaining permission from human participants to use their cells and tissues or otherwise include them in research studies. With informed consent, scientists can use human cells or tissues in experiments to learn more about the human body and to test new medicines. This article describes how these tissues are obtained, and the ethical concerns regarding the use of human tissues in research. The story of Henrietta Lacks and her immortal HeLa cell line is discussed, to demonstrate the importance of informed consent and to showcase Henrietta’s valuable contributions to research and modern medicine.
Percutaneous tibial nerve stimulation versus sacral nerve stimulation for the treatment of faecal incontinence
Authors: O’Connor Alexander , Reynolds Elizabeth , Molyneux Clare , Vasant Dipesh H. , Sharma Abhiram , Faulkner Gemma , McLaughlin John , Kiff Edward , Telford Karen
Published: January 2024

Introduction

Faecal incontinence (FI) is a common condition with a significant impact on quality of life (QoL). Neuromodulation treatments delivered by members of the multidisciplinary team including sacral nerve stimulation (SNS) and percutaneous tibial nerve stimulation (PTNS) are options for FI refractory to conservative management. The aim of this study was to assess whether a successful treatment with one neuromodulation modality corresponds with success in the other.

Methods

A retrospective review of a prospectively managed neuromodulation database identified 15 patients who had undergone both PTNS and SNS. The definition of success of each treatment was a >50% improvement in any of The St. Mark's Incontinence Score, Manchester Health Questionnaire, or weekly faecal urgency or FI episodes.

Results

Complete data from 12 patients was available for assessment and PTNS was delivered as the first treatment in nine patients. Overall, seven patients (58%) had successful PTNS treatment, with 10 (83%) having a successful SNS trials. Of the seven patients who had successful PTNS treatment, six patients (85.4%) went on to have success with SNS. Of the five patients who failed PTNS, four (80%) went on to have SNS success. Five (71%) of those who had positive PTNS outcomes had permanent SNS implantation as their final treatment decision.

Conclusion

This study suggests that there is no clear relationship between successful PTNS treatment and an SNS trial period which may be explained by differing mechanisms of action or the potential placebo effect of PTNS. Further work is required to investigate any association in larger studies to inform clinical practice.
Case report: Diagnosis and autogenous vaccine treatment of herpesvirus in a green turtle (Chelonia mydas) in Santa Marta, Colombia
Authors: Castro Lyda R. , Villalba-Viscaíno Vivian , Oviedo Ángel , Zambrano Edgar , Dávila Angela , Naranjo Gualberto , Oro-Genes Blanca De , Combatt Anthony , Prieto-Rodríguez Julieth , Ortiz Arnaldo , Villamizar Natalia
Published: January 2024

This study reports the first case of fibropapillomatosis (FP) in the green turtle Chelonia mydas that has been successfully diagnosed and treated in Colombia. Worldwide, FP has reached epizootic proportions as it has been reported in marine turtles of tropical and subtropical waters, and in severe cases, it reduces the probability of survival. Treatment has been elusive as multiple surgical excisions are needed due to tumor recurrence. In this case, one green turtle with multiple tumors was diagnosed by histopathology and molecular detection of the chelonid herpesvirus 5 (ChHV5) by means of amplification and sequencing of the DNA polymerase (DNApol) gene. Two separate treatments that consisted of autogenous vaccines and surgical excisions were applied; the first one had a partial success as one out of the tumors treated reappeared after 3 months post-treatment. Treatment 2 consisted of an autogenous vaccine enriched with adjuvants and applied at increasing doses, after which, the tumor significatively decreased in size and was surgically removed. At the end of the 6 months follow-up period, no tumor recurrence was observed, and the turtle was in apparent optimal health conditions. These findings, although limited, suggest a possible treatment that might help to contain this epizootic problem.
Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds
Authors: Gomez-Buendia Alberto , Alvarez Julio , Bezos Javier , Mourelo Jorge , Amado Javier , Saez Jose Luis , de Juan Lucia , Romero Beatriz
Published: January 2024

Non-tuberculous mycobacteria (NTM) are considered a relevant cause of non-specific reactions to the most widely applied bovine tuberculosis (bTB) test, the intradermal tuberculin test. In order to establish which NTM species might act as a potential source of such diagnostic interference, a collection of 373 isolates obtained from skin test positive cows from 359 officially tuberculosis-free (OTF) herds, culled in the framework of the bTB eradication campaign in Spain, were identified at the species level through PCR and Sanger sequencing of the 16S rDNA, hsp65 and rpoB genes. Of the 308 isolates for which a reliable identification was achieved, 32 different mycobacterial species were identified, with certain species being most represented: among M. avium complex members (n = 142, 46.1%), M. avium subsp. hominissuis (98; 69.0%) was the most abundant followed by M. avium subsp. avium (33, 23.2%), and M. intracellulare (7, 4.9%). Among non-MAC members (n = 166, 53.9%), M. nonchromogenicum (85; 27.6%) and M. bourgelatii (11; 5.6%) were the predominant species. In addition, mixed results were obtained in 53 isolates presenting up to 30 different genotypes, which could be indicative of new mycobacterial species. Our results represent a first step toward characterizing the diversity of NTM species that could interfere with official diagnostic tests for bTB eradication in Spain.
Program Code Generation with Generative AIs
Authors: Idrisov Baskhad , Schlippe Tim
Published: January 2024

Our paper compares the correctness, efficiency, and maintainability of human-generated and AI-generated program code. For that, we analyzed the computational resources of AI- and human-generated program code using metrics such as time and space complexity as well as runtime and memory usage. Additionally, we evaluated the maintainability using metrics such as lines of code, cyclomatic complexity, Halstead complexity and maintainability index. For our experiments, we had generative AIs produce program code in Java, Python, and C++ that solves problems defined on the competition coding website leetcode.com. We selected six LeetCode problems of varying difficulty, resulting in 18 program codes generated by each generative AI. GitHub Copilot, powered by Codex (GPT-3.0), performed best, solving 9 of the 18 problems (50.0%), whereas CodeWhisperer did not solve a single problem. BingAI Chat (GPT-4.0) generated correct program code for seven problems (38.9%), ChatGPT (GPT-3.5) and Code Llama (Llama 2) for four problems (22.2%) and StarCoder and InstructCodeT5+ for only one problem (5.6%). Surprisingly, although ChatGPT generated only four correct program codes, it was the only generative AI capable of providing a correct solution to a coding problem of difficulty level hard. In summary, 26 AI-generated codes (20.6%) solve the respective problem. For 11 AI-generated incorrect codes (8.7%), only minimal modifications to the program code are necessary to solve the problem, which results in time savings between 8.9% and even 71.3% in comparison to programming the program code from scratch.
A Multidisciplinary Optimization Framework for Ecodesign of Reusable Microsatellite Launchers
Authors: Musso Girolamo , Figueiras Iara , Goubel Héléna , Gonçalves Afonso , Costa Ana Laura , Ferreira Bruna , Azeitona Lara , Barata Simão , Souza Alain , Afonso Frederico , Ribeiro Inês , Lau Fernando
Published: January 2024

The commercial space launch sector is currently undergoing a significant shift, with increasing competition and demand for launch services, as well as growing concerns about the environmental impact of rocket launches. To address these challenges, within the New Space Portugal project scope, a multidisciplinary framework for designing and optimizing new launch vehicles is proposed. Creating a more resilient and responsible space industry can be achieved by combining technological innovation and environmental sustainability, as emphasized by the framework. The main scope of the framework was to couple all the disciplines relevant to the space vehicle design in a modular way. Significant emphasis was placed on the infusion of ecodesign principles, including Life Cycle Assessment (LCA) considerations. Optimization techniques were employed to enhance the design and help designers conduct trade-off studies. In general, this multidisciplinary framework aims to provide a comprehensive approach to designing next-generation launch vehicles that meet the demands of a rapidly changing market while also minimizing their environmental impact. A methodology that leverages the strengths of both genetic and gradient-based algorithms is employed for optimizations with the objectives of maximizing the apogee altitude and minimizing the Global Warming Potential (GWP). Despite only being tested at the moment for sounding rockets, the framework has demonstrated promising results. It has illuminated the potential of this approach, leading to the identification of three optimal designs: one for maximizing the apogee, another for minimizing GWP, and a compromise design that strikes a balance between the two objectives. The outcomes yielded a maximum apogee of 6.41 km, a minimum GWP of 9.06 kg CO2eq, and a balanced compromise design featuring an apogee of 5.75 km and a GWP of 25.64 kg CO2eq.
Long-Term Network Structure Evolution Investigation for Sustainability Improvement: An Empirical Analysis on Global Top Full-Service Carriers
Authors: Yang Wendong , Jiang Yun , Chi Yulin , Xu Zhengjia , Wei Wenbin
Published: January 2024

The continuous and strategic planning of full-service carriers plays a prominent role in transferring and adapting them into resilient full-service carrier network structures. The exploration of full-service carrier network structures using the latest long-term empirical data facilitates enhancing cognitive capabilities in aspects of identifying network development tendencies, readjusting network structures, and supporting determinations of strategic business routes. Aiming at providing sustainable transport network solutions with historical long-term network structure analysis, this paper researches the global top 10 full-service carriers’ air transport networks from 2007 to 2022, applied using social network analysis (SNA). The static metrics from local to path-based perspectives are adopted to explore the global network evolution trend, along with competitiveness characteristics over critical airports. The cascading failure model is applied as a key indicator to analyze the dynamic robustness capability for the network. The similarity changing feature among the selected networks over the past years from 2007 to 2022 is measured using the autocorrelation function (ACF). The results indicate that, from 2011 to 2019, the majority of full-service carrier networks belong to the network types of closed, structural symmetry and two-way transitivity. The critical airports in North America present superiority in terms of network efficiency over those in Europe, Asia, and Oceania. The 10 full-service carriers’ air transport networks all show the trend of being more destruction-resistant. During the COVID-19 pandemic period, the merger with other airlines and the signing of a joint venture agreement led to higher temporal variability in the network structure.
Evaluating the Anesthetic and Physiologic Effects of Intramuscular and Intravenous Alfaxalone in Eastern Mud Turtles (Kinosternon subrubrum)
Authors: Zec Stephanie , Mitchell Mark A. , Rockwell Kelly , Lindemann Dana
Published: January 2024

Current sedation protocols for chelonians can pose a challenge to clinicians because of prolonged induction and recovery times, difficulties in gaining venous access, and natural species variation. This study evaluated the sedative and physiologic effects of intramuscular (IM) and intravenous (IV) alfaxalone in six wild-caught adult eastern mud turtles (Kinosternon subrubrum). The turtles received alfaxalone 10 mg/kg IM and IV in a randomized cross-over design. A 10-day washout period occurred between trials. Baseline parameters (heart rate, respiratory rate, temperature, and reflexes) were assessed prior to injection and every 5 min post-injection until recovery. Three venous blood gas samples were also collected and analyzed over the course of each trial (baseline, induction, and recovery). Intravenous alfaxalone resulted in a significantly faster induction (p = 0.016; median: 1.5 min, 25–75%: 1–7.5, minimum–maximum: 1–21) and a shorter total sedation time (p = 0.041; median: 52 min, 25–75%: 34.5–62.5, minimum–maximum: 33–87) when compared with IM alfaxalone (induction, median: 20 min, 25–75%: 15–22.5, minimum–maximum: 15–25; total, median: 70 min, 25–75%: 65–82.5, minimum–maximum: 65–90). Blood gas and physiologic parameters were not significantly different between groups; however, the pH (p = 0.009) and glucose (p = 0.0001) significantly increased, and partial pressure of carbon dioxide (p = 0.024) significantly decreased over time. This study demonstrated that alfaxalone 10 mg/kg IV or IM can be used to provide safe and effective sedation in eastern mud turtles.
Local and Systemic Inflammation in Finnish Dairy Cows with Digital Dermatitis
Authors: Pirkkalainen Hertta , Riihimäki Aino , Lienemann Taru , Anttila Marjukka , Kujala-Wirth Minna , Rajala-Schultz Päivi , Simojoki Heli , Soveri Timo , Orro Toomas
Published: January 2024

Digital dermatitis is a disease of the digital skin and causes lameness and welfare problems in dairy cattle. This study assessed the local and systemic inflammatory responses of cows with different digital dermatitis lesions and compared macroscopical and histological findings. Cow feet (n = 104) were evaluated macroscopically and skin biopsies histologically. Serum samples were analyzed for acute phase proteins (serum amyloid A and haptoglobin) and pro-inflammatory cytokines (interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha). Cows with macroscopically graded active lesions (p = 0.028) and non-active lesions (p = 0.008) had higher interleukin-1 beta levels in their serum compared to healthy cows. Interleukin-1 beta serum concentrations were also higher (p = 0.042) when comparing lesions with necrosis to lesions without necrosis. There was no difference when other cytokine or acute phase protein concentrations in healthy cows were compared to those in cows with different digital dermatitis lesions. A novel histopathological grading was developed based on the chronicity of the lesions and presence of necrosis and ulceration. The presence and number of spirochetes were graded separately. In the most severe chronic lesions, there was marked epidermal hyperplasia and hyperkeratosis with necrosis, deep ulceration, and suppurative inflammation. Spirochetes were found only in samples from necrotic lesions. This study established that digital dermatitis activates proinflammatory cytokines. However, this did not initiate the release of acute phase proteins from the liver. A histopathological grading that takes into account the age and severity of the lesions and presence of spirochetes was developed to better understand the progression of the disease. It is proposed that necrosis of the skin is a result of ischemic necrosis following reduced blood flow in the dermal papillae due to pressure and shear stress caused by thickened epidermis, and that the spirochetes are secondary invaders following tissue necrosis.
Single-Cell RNA Sequencing Reveals Differences in Chromatin Remodeling and Energy Metabolism among In Vivo-Developed, In Vitro-Fertilized, and Parthenogenetically Activated Embryos from the Oocyte to 8-Cell Stages in Pigs
Authors: Fan Jianlin , Liu Chang , Zhao Yunjing , Xu Qianqian , Yin Zhi , Liu Zhonghua , Mu Yanshuang
Published: January 2024

In vitro-fertilized (IVF) and parthenogenetically activated (PA) embryos, key to genetic engineering, face more developmental challenges than in vivo-developed embryos (IVV). We analyzed single-cell RNA-seq data from the oocyte to eight-cell stages in IVV, IVF, and PA porcine embryos, focusing on developmental differences during early zygotic genome activation (ZGA), a vital stage for embryonic development. (1) Our findings reveal that in vitro embryos (IVF and PA) exhibit more similar developmental trajectories compared to IVV embryos, with PA embryos showing the least gene diversity at each stage. (2) Significant differences in maternal mRNA, particularly affecting mRNA splicing, energy metabolism, and chromatin remodeling, were observed. Key genes like SMARCB1 (in vivo) and SIRT1 (in vitro) played major roles, with HDAC1 (in vivo) and EZH2 (in vitro) likely central in their complexes. (3) Across different types of embryos, there was minimal overlap in gene upregulation during ZGA, with IVV embryos demonstrating more pronounced upregulation. During minor ZGA, global epigenetic modification patterns diverged and expanded further. Specifically, in IVV, genes, especially those linked to H4 acetylation and H2 ubiquitination, were more actively regulated compared to PA embryos, which showed an increase in H3 methylation. Additionally, both types displayed a distinction in DNA methylation. During major ZGA, IVV distinctively upregulated genes related to mitochondrial regulation, ATP synthesis, and oxidative phosphorylation. (4) Furthermore, disparities in mRNA degradation-related genes between in vivo and in vitro embryos were more pronounced during major ZGA. In IVV, there was significant maternal mRNA degradation. Maternal genes regulating phosphatase activity and cell junctions, highly expressed in both in vivo and in vitro embryos, were degraded in IVV in a timely manner but not in in vitro embryos. (5) Our analysis also highlighted a higher expression of many mitochondrially encoded genes in in vitro embryos, yet their nucleosome occupancy and the ATP8 expression were notably higher in IVV.
Exploring Andalusia’s Rich Heritage through Surveys: Pastoral Livestock Farming as a Tourist Attraction Resource
Authors: Ruiz Morales Francisco de Asís , Cruz Moriana Verónica , Bermúdez Rus María , Mancilla-Leytón Juan Manuel , Ureña Cámara Luis Pablo
Published: January 2024

This study aimed to analyze the interest, willingness to pay, and profile of tourists participating in specific agrotourism activities linked to extensive livestock farming in order to encourage the development of those activities that are most demanded by tourists, as well as to support the proposals for improvements to promote this kind of tourism in the region of Andalusia (Spain). For this purpose, a survey was conducted, which was organized into six sections: (i) sociodemographic data, (ii) general aspects of tourism, (iii) rural tourism, (iv) livestock farming and tourism, (v) benefits of extensive livestock farming, and (vi) tourism activities linked to livestock farming. A total of 892 responses were received, out of which 753 were analyzed. The results show that the respondents are interested or motivated by the proposed activities. The most attractive activities are those related to livestock farming, which involve some kind of workshop on the production of handicrafts. Despite tourists having low-to-medium knowledge of extensive livestock farming, they all express a highly positive perception of the associated attributes. Developing strategies to enhance the willingness to pay for agrotourism activities is crucial, with the overall experience being a key element of their success.
Characterizing the Effect of Campylobacter jejuni Challenge on Growth Performance, Cecal Microbiota, and Cecal Short-Chain Fatty Acid Concentrations in Broilers
Authors: Al Hakeem Walid G. , Cason Emily E. , Adams Daniel , Fathima Shahna , Shanmugasundaram Revathi , Lourenco Jeferson , Selvaraj Ramesh K.
Published: January 2024

This study aimed to understand the effect of C. jejuni challenge on the cecal microbiota and short-chain fatty acid (SCFA) concentration to form a better understanding of the host–pathogen interaction. Sixty broilers were randomly allocated into two treatments: control and challenge. Each treatment was replicated in six pens with five birds per pen. On day 21, birds in the challenge group were orally gavaged with 1 × 108C. jejuni/mL, while the control group was mock challenged with PBS. The C. jejuni challenge had no effect on body weight, feed intake, and feed conversion ratio compared to the control group. On day 28, the C. jejuni challenge decreased the observed features and Shannon index compared to the control group. On the species level, the C. jejuni challenge decreased (p = 0.02) the relative abundance of Sellimonas intestinalis on day 28 and increased (p = 0.04) the relative abundance of Faecalibacterium sp002160895 on day 35 compared to the control group. The C. jejuni challenge did not change the microbial function and the cecal concentrations of SCFA on days 28 and 35 compared to the control group. In conclusion, C. jejuni might alter the gut microbiota’s composition and diversity without significantly compromising broilers’ growth.
Culture-Independent Quantification of Legionella pneumophila in Evaporative Cooling Systems Using Immunomagnetic Separation Coupled with Flow Cytometry
Authors: Streich Philipp , Redwitz Johannes , Walser-Reichenbach Sandra , Herr Caroline E. W. , Elsner Martin , Seidel Michael
Published: January 2024

Legionella pneumophila are pathogenic bacteria that repeatedly occur in high concentrations in the process water of evaporative cooling systems (ECS). When released into the environment, the resulting bioaerosols can cause outbreaks with fatal consequences. The official, internationally accepted detection method for Legionella spp. in water samples is based on cultivation. However, cultivation is time-consuming and may underestimate the total count of viable L. pneumophila in ECS. Therefore, culture-independent methods are receiving attention for rapid monitoring. Cartridge-based immunomagnetic separation (IMS) coupled with flow cytometry (FCM) is an innovative, antibody-based method for the culture-independent quantification of L. pneumophila, using a panel of antibodies against serogroup (Sg) 1–15. We characterized the IMS-FCM method as a quantitative rapid test by general analytical procedures. Viable cryopreserved L. pneumophila standards were used in calibration experiments for the method. We achieved detection limits for Sg 1, Sg 4, and Sg 6 of 100, 105 and 88 viable cells per 100 mL, respectively. Furthermore, we demonstrated the practical applicability of IMS-FCM with real ECS samples and compared the performance against cultivation. Cultivation showed here no positive results, but IMS-FCM evidenced L. pneumophila in a range of 0–80,000 viable cells per 100 mL. This work demonstrates that IMS-FCM is a suitable, culture-independent, quantitative method for rapidly monitoring L. pneumophila.