bluuebunny/crossref_metadata_2025 huggingface dataset:
Improving the signal-to-noise ratio of computational ghost imaging of a reflective object with a rough surface by Hadamard modulated light field
Authors: Lu Mingshu , Zou Xuanpengfan , Zhou Liyu , Nan Suqin , Jiang Teng , Huang Xianwei , Tan Wei , Bai Yanfeng , Fu Xiquan
Published: December 2021

We propose an optimization scheme to improve the reconstruction quality of computational ghost imaging (GI) of a reflective target with a rough surface by using the Hadamard modulation light field (HCGI). By comparison with computational GI with a traditional Gaussian light field (GCGI), the signal-to-noise ratio of GCGI is quite bad, and it is difficult to distinguish the imaging signal from the background when the surface roughness of the object is higher, while a ghost image with better quality can be obtained by HCGI. The difference is explained by comparing the distribution of the correlation coefficient. Additionally, it is found that HCGI has better noise robustness in comparison with GI with other random coded patterns.
Designing an ultra-thin and wideband low-frequency absorber based on lumped resistance
Authors: Du Zhiqiang , Liang Jiangang , Cai Tong , Wang Guangming , Deng Taowu , Wu Borui
Published: December 2021

Traditional absorbers are mostly limited by their large size and high profile, which renders them unfavorable for practical devices. To solve this problem, we design and test an ultra-thin metamaterial absorber (UTMA). The top layer of the metamaterial absorber is designed as a patterned combination of split ring and metal strips, so that its resonant frequency point is in the target low frequency. Meanwhile, ohmic loss is enhanced by loading lumped resistance in the gap of the meta-surface to improve the absorb efficiency (> 90%) and to expand the working bandwidth (1.24–3.14 GHz). Moreover, the total thickness of the absorber is 9 mm (0.037λwith respect to the lowest operating frequency). The working mechanism of UTMA is analyzed based on the equivalent media theory, surface current and electric field energy distribution. The experimental results are in good agreement with the simulation, which verifies the feasibility of the design. In this work, the metamaterial absorber is designed to meet the target requirements from three performance indexes: low frequency, ultra-thin, and wideband, leading to the prospect of broad applications in the military and civil fields.
Ultra-compact, efficient and high-polarization-extinction-ratio polarization beam splitters based on photonic anisotropic metamaterials
Authors: Zhang Jingjing , Shi Xiaodong , Zhang Zhaojian , Guo Kai , Yang Junbo
Published: December 2021

Anisotropic metameterials (AM) provide a new avenue for a next-generation silicon platform to design ultra-compact, densely integrated optical components, thus functional devices based on AM are drawing increasing attention recently. Here, we propose a novel efficient polarization beam splitter (PBS) with high polarization extinction ratio based on AM. An ultra-compact coupling region of 2.5 × 14 µm2 is achieved by tailoring the AM structures, which can efficiently suppress the TE mode coupling, and enhance the TM mode coupling in the directional couplers simultaneously. The insertion loss is simulated to be as low as <0.2 dB within a bandwidth of 70 nm for both modes, and the polarization extinction ratio is as high as 46 dB and 33 dB for TE and TM modes, respectively. We also experimentally demonstrate the proposed PBS, with low insertion loss of 1 dB , high extinction ratio of >20 dB and wide operational bandwidth of >80 nm.
Direct femtosecond laser inscription of Bragg gratings in Ho3+/Pr3+ co-doped AlF3-based glass fibers for a 2.86 µm laser
Authors: Xu Niannian , Yang Zhiyong , Zhang Jiquan , Lv Nian , Liu Mo , Wang Ruoning , Li Zhenrui , Jia Shijie , Brambilla Gilberto , Wang Shunbin , Wang Pengfei
Published: December 2021

In this Letter, we report the fabrication of fiber Bragg gratings (FBGs) in home-made Ho3+/Pr3+ co-doped single-cladding fluoroaluminate (AlF3) glass fibers and its application in watt-level lasing at the mid-infrared (MIR) wavelength of 2.86 µm. The FBGs were inscribed using an 800 nm femtosecond (fs) laser direct-writing technique. The FBG properties were investigated for different pulse energies, inscription speeds, grating orders, and transversal lengths. A second-order FBG with a high reflectivity of 99% was obtained at one end of a 16.5-cm-long gain fiber. Under 1150 nm laser pumping, this fiber yielded a power exceeding 1 W at 2863.9 nm with an overall laser efficiency of 17.7%. The fiber laser showed a FWHM bandwidth of 0.46 nm and long-term spectral stability.
Evaluation of PGP 9.5, NGFR, TGFβ1, FGFR1, MMP-2, AT2R2, SHH, and TUNEL in Primary Obstructive Megaureter Tissue
Authors: Junga Anna , Siņicins Ivo , Pētersons Aigars , Pilmane Māra
Published: December 2021

Primary obstructive megaureter (POM) morphogenesis is not fully known. The aim of the study was to evaluate the appearance of different factors that might take part in the pathogenesis of POM. Megaureter tissues of 14 children were stained with hematoxylin and eosin as well as with immunohistochemistry for protein gene product 9.5, nerve growth factor receptor, transforming growth factor beta 1 (TGFβ1), fibroblast growth factor receptor 1 (FGFR1), matrix metalloproteinase 2 (MMP-2), angiotensin 2 receptor type 2, and sonic hedgehog (SHH) protein. Apoptosis was detected by terminal dUTP nick-end labeling reaction. POM tissues revealed transitional epithelium with scattered vacuolization, submucosa with inflammatory cells, and focally vacuolized and chaotically organized muscle layers. Apoptosis, appearance of MMP-2, FGFR1, and SHH prevailed, but TGFβ1 positive cell number was lower in patients. Correlation between MMP-2 in epithelium and endothelium, FGFR1 and MMP-2 in epithelium, and TGFβ1 in epithelium and connective tissue in patients was detected. POM morphopathogenesis involves an apoptotic cell death of epithelium and smooth muscle as well as tissue degradation in epithelium and connective tissue of the ureter wall. The decrease of tissue growth through diminished TGFβ1 expression and stimulation of FGFR1 and MMP-2 suggests a disbalance of tissue remodelation in the megaureter wall:
Preparing to move: Setting initial conditions to simplify interactions with complex objects
Authors: Nayeem Rashida , Bazzi Salah , Sadeghi Mohsen , Hogan Neville , Sternad Dagmar
Published: December 2021

Humans dexterously interact with a variety of objects, including those with complex internal dynamics. Even in the simple action of carrying a cup of coffee, the hand not only applies a force to the cup, but also indirectly to the liquid, which elicits complex reaction forces back on the hand. Due to underactuation and nonlinearity, the object’s dynamic response to an action sensitively depends on its initial state and can display unpredictable, even chaotic behavior. With the overarching hypothesis that subjects strive for predictable object-hand interactions, this study examined how subjects explored and prepared the dynamics of an object for subsequent execution of the target task. We specifically hypothesized that subjects find initial conditions that shorten the transients prior to reaching a stable and predictable steady state. Reaching a predictable steady state is desirable as it may reduce the need for online error corrections and facilitate feed forward control. Alternative hypotheses were that subjects seek to reduce effort, increase smoothness, and reduce risk of failure. Motivated by the task of ‘carrying a cup of coffee’, a simplified cup-and-ball model was implemented in a virtual environment. Human subjects interacted with this virtual object via a robotic manipulandum that provided force feedback. Subjects were encouraged to first explore and prepare the cup-and-ball before initiating a rhythmic movement at a specified frequency between two targets without losing the ball. Consistent with the hypotheses, subjects increased the predictability of interaction forces between hand and object and converged to a set of initial conditions followed by significantly decreased transients. The three alternative hypotheses were not supported. Surprisingly, the subjects’ strategy was more effortful and less smooth, unlike the observed behavior in simple reaching movements. Inverse dynamics of the cup-and-ball system and forward simulations with an impedance controller successfully described subjects’ behavior. The initial conditions chosen by the subjects in the experiment matched those that produced the most predictable interactions in simulation. These results present first support for the hypothesis that humans prepare the object to minimize transients and increase stability and, overall, the predictability of hand-object interactions.
Case-area targeted preventive interventions to interrupt cholera transmission: Current implementation practices and lessons learned
Authors: Sikder Mustafa , Altare Chiara , Doocy Shannon , Trowbridge Daniella , Kaur Gurpreet , Kaushal Natasha , Lyles Emily , Lantagne Daniele , Azman Andrew S. , Spiegel Paul
Published: December 2021

Background

Cholera is a major cause of mortality and morbidity in low-resource and humanitarian settings. It is transmitted by fecal-oral route, and the infection risk is higher to those living in and near cholera cases. Rapid identification of cholera cases and implementation of measures to prevent subsequent transmission around cases may be an efficient strategy to reduce the size and scale of cholera outbreaks.

Methodology/Principle findings

We investigated implementation of cholera case-area targeted interventions (CATIs) using systematic reviews and case studies. We identified 11 peer-reviewed and eight grey literature articles documenting CATIs and completed 30 key informant interviews in case studies in Democratic Republic of Congo, Haiti, Yemen, and Zimbabwe. We documented 15 outbreaks in 12 countries where CATIs were used. The team composition and the interventions varied, with water, sanitation, and hygiene interventions implemented more commonly than those of health. Alert systems triggering interventions were diverse ranging from suspected cholera cases to culture confirmed cases. Selection of high-risk households around the case household was inconsistent and ranged from only one case to approximately 100 surrounding households with different methods of selecting them. Coordination among actors and integration between sectors were consistently reported as challenging. Delays in sharing case information impeded rapid implementation of this approach, while evaluation of the effectiveness of interventions varied.

Conclusions/Significance

CATIs appear effective in reducing cholera outbreaks, but there is limited and context specific evidence of their effectiveness in reducing the incidence of cholera cases and lack of guidance for their consistent implementation. We propose to 1) use uniform cholera case definitions considering a local capacity to trigger alert; 2) evaluate the effectiveness of individual or sets of interventions to interrupt cholera, and establish a set of evidence-based interventions; 3) establish criteria to select high-risk households; and 4) improve coordination and data sharing amongst actors and facilitate integration among sectors to strengthen CATI approaches in cholera outbreaks.
Evaluation of multi-assay algorithms for identifying individuals with recent HIV infection: HPTN 071 (PopART)
Authors: Grant-McAuley Wendy , Klock Ethan , Laeyendecker Oliver , Piwowar-Manning Estelle , Wilson Ethan , Clarke William , Breaud Autumn , Moore Ayana , Ayles Helen , Kosloff Barry , Shanaube Kwame , Bock Peter , Mandla Nomtha , van Deventer Anneen , Fidler Sarah , Donnell Deborah , Hayes Richard , Eshleman Susan H. ,   for the HPTN 071 (PopART) Study Team
Published: December 2021

Background

Assays and multi-assay algorithms (MAAs) have been developed for population-level cross-sectional HIV incidence estimation. These algorithms use a combination of serologic and/or non-serologic biomarkers to assess the duration of infection. We evaluated the performance of four MAAs for individual-level recency assessments.

Methods

Samples were obtained from 220 seroconverters (infected <1 year) and 4,396 non-seroconverters (infected >1 year) enrolled in an HIV prevention trial (HPTN 071 [PopART]); 28.6% of the seroconverters and 73.4% of the non-seroconverters had HIV viral loads ≤400 copies/mL. Samples were tested with two laboratory-based assays (LAg-Avidity, JHU BioRad-Avidity) and a point-of-care assay (rapid LAg). The four MAAs included different combinations of these assays and HIV viral load. Seroconverters on antiretroviral treatment (ART) were identified using a qualitative multi-drug assay.

Results

The MAAs identified between 54 and 100 (25% to 46%) of the seroconverters as recently-infected. The false recent rate of the MAAs for infections >2 years duration ranged from 0.2%-1.3%. The MAAs classified different overlapping groups of individuals as recent vs. non-recent. Only 32 (15%) of the 220 seroconverters were classified as recent by all four MAAs. Viral suppression impacted the performance of the two LAg-based assays. LAg-Avidity assay values were also lower for seroconverters who were virally suppressed on ART compared to those with natural viral suppression.

Conclusions

The four MAAs evaluated varied in sensitivity and specificity for identifying persons infected <1 year as recently infected and classified different groups of seroconverters as recently infected. Sensitivity was low for all four MAAs. These performance issues should be considered if these methods are used for individual-level recency assessments.
COVID-19 affected the food behavior of different age groups in Chinese households
Authors: Chen Ting , Wang Chong , Cui Zhenling , Liu Xiaojie , Jiang Jun , Yin Jun , Feng Huajun , Dou Zhengxia
Published: December 2021

The COVID-19 pandemic brought profound changes to all corners of society and affected people in every aspect of their lives. This survey-based study investigated how household food related matters such as food sourcing and consumption behaviors of 2,126 Chinese consumers in different age groups changed approximately two months into the COVID-19 quarantine. A new food sourcing mechanism, community-based online group grocery-ordering (CoGGO), was widely adopted by households, particularly among the youngest group studied (18–24 years of age). The same group showed a higher confidence in the food supply system during the quarantine and a greater propensity for weight gain while staying-at-home. The more mature age group (≥35 years of age) showed heightened vigilance and awareness, with fewer grocery-shopping trips, a higher tendency for purchasing extra food, and less tendency to waste food. Survey findings of the new food-sourcing mechanism, attitudes to food, and changes in behavior among different age groups provide valuable insights to guide policies and management interventions to address matters pertaining to food supply and distribution, food access and household food security, and food waste reduction.
KRSA: An R package and R Shiny web application for an end-to-end upstream kinase analysis of kinome array data
Authors: DePasquale Erica A. K. , Alganem Khaled , Bentea Eduard , Nawreen Nawshaba , McGuire Jennifer L. , Tomar Tushar , Naji Faris , Hilhorst Riet , Meller Jaroslaw , McCullumsmith Robert E.
Published: December 2021

Phosphorylation by serine-threonine and tyrosine kinases is critical for determining protein function. Array-based platforms for measuring reporter peptide signal levels allow for differential phosphorylation analysis between conditions for distinct active kinases. Peptide array technologies like the PamStation12 from PamGene allow for generating high-throughput, multi-dimensional, and complex functional proteomics data. As the adoption rate of such technologies increases, there is an imperative need for software tools that streamline the process of analyzing such data. We present Kinome Random Sampling Analyzer (KRSA), an R package and R Shiny web-application for analyzing kinome array data to help users better understand the patterns of functional proteomics in complex biological systems. KRSA is an All-In-One tool that reads, formats, fits models, analyzes, and visualizes PamStation12 kinome data. While the underlying algorithm has been experimentally validated in previous publications, we demonstrate KRSA workflow on dorsolateral prefrontal cortex (DLPFC) in male (n = 3) and female (n = 3) subjects to identify differential phosphorylation signatures and upstream kinase activity. Kinase activity differences between males and females were compared to a previously published kinome dataset (11 female and 7 male subjects) which showed similar global phosphorylation signals patterns.
Flooding and ecological restoration promote wetland microbial communities and soil functions on former cranberry farmland
Authors: Rubin Rachel L. , Ballantine Kate A. , Hegberg Arden , Andras Jason P.
Published: December 2021

Microbial communities are early responders to wetland degradation, and instrumental players in the reversal of this degradation. However, our understanding of soil microbial community structure and function throughout wetland development remains incomplete. We conducted a survey across cranberry farms, young retired farms, old retired farms, flooded former farms, ecologically restored former farms, and natural reference wetlands with no history of cranberry farming. We investigated the relationship between the microbial community and soil characteristics that restoration intends to maximize, such as soil organic matter, cation exchange capacity and denitrification potential. Among the five treatments considered, flooded and restored sites had the highest prokaryote and microeukaryote community similarity to natural wetlands. In contrast, young retired sites had similar communities to farms, and old retired sites failed to develop wetland microbial communities or functions. Canonical analysis of principal coordinates revealed that soil variables, in particular potassium base saturation, sodium, and denitrification potential, explained 45% of the variation in prokaryote communities and 44% of the variation in microeukaryote communities, segregating soil samples into two clouds in ordination space: farm, old retired and young retired sites on one side and restored, flooded, and natural sites on the other. Heat trees revealed possible prokaryotic (Gemmatimonadetes) and microeukaryotic (Rhizaria) indicators of wetland development, along with a drop in the dominance ofNucletmyceain restored sites, a class that includes suspected mycorrhizal symbionts of the cranberry crop. Flooded sites showed the strongest evidence of wetland development, with triple the soil organic matter accumulation, double the cation exchange capacity, and seventy times the denitrification potential compared to farms. However, given that flooding does not promote any of the watershed or habitat benefits as ecological restoration, we suggest that flooding can be used to stimulate beneficial microbial communities and soil functions during the restoration waiting period, or when restoration is not an option.
Using mathematical models to evaluate germination rate and seedlings length of chickpea seed (Cicer arietinum L.) to osmotic stress at cardinal temperatures
Authors: Shah Sikandar , Ullah Sami , Ali Sajjad , Khan Ajmal , Ali Muhammad , Hassan Said
Published: December 2021

Cicer arietinum is the 3rd most important cool season legume crop growing in vast arid and semi-arid regions of the world. A lab experiment was designed using hydrothermal time model (HTT) to investigate the chickpea seed germination (SG) behavior, cardinal temperatures and germination responses across fluctuating temperatures (Ts) and water potentials (Ψs). Seeds of chickpea var. NIFA 1995 were germinated at six constant Ts (7, 14, 21, 28, 35 and 42°C) each having the following five water potentials: 0, -0.2, -0.4–0.6 and -0.8 MPa. Germination percentage (G%) decreased significantly at (*P ≤ 0.05) from 86.7% at 28°C in -0.2 MPa to 10% in -0.2 MPa at 7°C. The germination rate (GR = 1/t50) against different T percentiles exhibited that linear increase was observed in the GR pattern above and below the To. Based on the confidence intervals of the model coefficients and (R2: 0.96), the average cardinal temperatures were 4.7, 23 and 44.2°C for the base (Tb), optimal (To) and ceiling (Tc) temperatures respectively. θT1 value was observed maximum at 28°C in -0.2 MPa and decreases with decreasing Ψ (-0.8 MPa). In comparison with control, the θT2 value was also highest in -0.2 MPa at 28°C. The thermal time (TT) concept is well fitted to germination fraction data in distilled water with an R2 value increasing 0.972. The hydro time constant (θH) increased with an increase in T to To and then decreased when T>To. The ѱb(50) irregularly varied with increasing T, σΨb was also recorded lowest (0.166 MPa) at 28°C and highest (0.457 MPa) at 7°C. Based on the statistical analysis, cardinal temperatures, hydrothermal time constant (θHTT) and germination findings the HTT gives an insight into the interactive effect of T and Ψ on seed germination time courses under varying environmental conditions.
Investigation on spatial variability and influencing factors of drinking water iodine in Xinjiang, China
Authors: Yang Zhen , Wang Chenchen , Nie Yanwu , Sun Yahong , Tian Maozai , Ma Yuhua , Zhang Yuxia , Yuan Yimu , Zhang Liping
Published: December 2021

Background and objectives

Xinjiang is one of the areas in China with extremely severe iodine deficiency. The health of Xinjiang residents has been endangered for a long time. In order to provide reasonable suggestions for scientific iodine supplementation and improve the health and living standards of the people in Xinjiang, it is necessary to understand the spatial distribution of iodine content in drinking water and explore the influencing factors of spatial heterogeneity of water iodine content distribution.

Methods

The data of iodine in drinking water arrived from the annual water iodine survey in Xinjiang in 2017. The distribution of iodine content in drinking water in Xinjiang is described from three perspectives: sampling points, districts/counties, and townships/streets. ArcGIS was used for spatial auto-correlation analysis, mapping the distribution of iodine content in drinking water and visualizing the distribution of Geographically Weighted Regression (GWR) model parameter. Kriging method is used to predict the iodine content in water at non-sampling points. GWR software was used to build GWR model in order to find the factors affecting the distribution of iodine content in drinking water.

Results

There are 3293 sampling points in Xinjiang. The iodine content of drinking water ranges from 0 to 128 μg/L, the median is 4.15 μg/L. The iodine content in 78.6% of total sampling points are less than 10 μg/L, and only that in the 3.4% are more than 40 μg/L. Among 1054 towns’ water samples in Xinjiang, 88.9% of the samples’ water iodine content is less than 10 μg/L. Among the 94 studied areas, the median iodine content in drinking water in 87 areas was less than 10 μg/L, those values in 7 areas were between 10–40 μg/L, and the distribution of water iodine content in Xinjiang shows clustered. The GWR model established had found that the effects of soil type and precipitation on the distribution of iodine content in drinking water were statistically significant.

Conclusions

The iodine content of drinking water in Xinjiang is generally low, but there are also some areas which their drinking water has high iodine content. Soil type and precipitation are the factors affecting the distribution of drinking water iodine content, and are statistically significant (P<0.05).
Translating best practice into real practice: Methods, results and lessons from a project to translate an English sexual health survey into four Asian languages
Authors: Vujcich Daniel , Roberts Meagan , Gu Zhihong , Kao Shih-Chi , Lobo Roanna , Mao Limin , Oudih Enaam , Phoo Nang Nge Nge , Wong Horas , Reid Alison
Published: December 2021

Background

Migrants are underrepresented in population health surveys. Offering translated survey instruments has been shown to increase migrant representation. While ‘team translation’ represents current best practice, there are relatively few published examples describing how it has been implemented. The purpose of this paper is to document the process, results and lessons from a project to translate an English-language sexual health and blood-borne virus survey into Khmer, Karen, Vietnamese and Traditional Chinese.

Methods

The approach to translation was based on the TRAPD (Translation, Review, Adjudication, Pretesting, and Documentation) model. The English-language survey was sent to two accredited, independent translators. At least one bilingual person was chosen to review and compare the translations and preferred translations were selected through consensus. Agreed translations were pretested with small samples of individuals fluent in the survey language and further revisions made.

Results

Of the 51 survey questions, only nine resulted in identical independent translations in at least one language. Material differences between the translations related to: (1) the translation of technical terms and medical terminology (e.g. HIV); (2) variations in dialect; and (3) differences in cultural understandings of survey concepts (e.g. committed relationships).

Conclusion

Survey translation is time-consuming and costly and, as a result, deviations from TRAPD ‘best practice’ occurred. It is not possible to determine whether closer adherence to TRAPD ‘best practice’ would have improved the quality of the resulting translations. However, our study does demonstrate that even adaptations of the TRAPD method can identify issues that may not have been apparent had non-team-based or single-round translation approaches been adopted. Given the dearth of clear empirical evidence about the most accurate and feasible method of undertaking translations, we encourage future researchers to follow our example of making translation data publicly available to enhance transparency and enable critical appraisal.
The economic impact of rostering junior doctors to triage to assist nursing staff in the early part of the patient journey through the emergency department
Authors: Brain David , Johnson David , Hocking Julia , Chang Angela T.
Published: December 2021

Objective

This study aims to determine whether redeploying junior doctors to assist at triage represents good value for money and a good use of finite staffing resources.

Methods

We undertook a cost-minimisation analysis to produce new evidence, from an economic perspective, about the costs associated with reallocating junior doctors in the emergency department. We built a decision-analytic model, using a mix of prospectively collected data, routinely collected administrative databases and hospital costings to furnish the model. To measure the impact of uncertainty on the model’s inputs and outputs, probabilistic sensitivity analysis was undertaken, using Monte Carlo simulation.

Results

The mean costs for usual care were $27,035 (95% CI $27,016 to $27,054), while the mean costs for the new model of care were $25,474, (95% CI $25,453 to $25,494). As a result, the mean difference was -$1,561 (95% CI -$1,533 to -$1,588), with the new model of care being a less costly approach to managing staffing allocations, in comparison to the usual approach.

Conclusion

Our study shows that redeploying a junior doctor from the fast-track area of the department to assist at triage provides a modest reduction in cost. Our findings give decision-makers who seek to maximise benefit from their finite budget, support to reallocate personnel within the ED.
Innovations, contestations and fragilities of the health system response to COVID-19 in the Gauteng Province of South Africa
Authors: Rispel Laetitia C. , Marshall Carol , Matiwane Busisiwe , Tenza Immaculate Sabelile
Published: December 2021

Background

Gauteng province, with 26.3% of South Africa’s population, is the commercial and industrial powerhouse of the country. During the first epidemic wave in 2020, Gauteng accounted for 32.0% of South Africa’s reported COVID-19 cases.

Aim

The aim of this study was to describe the health system response to the COVID-19 pandemic during the first epidemic wave in Gauteng province and to explore the perspectives of key informants on the provincial response.

Material and methods

Using an adapted Pandemic Emergency Response Conceptual Framework, this was a qualitative case study design consisting of 36 key informant interviews and a document analysis. We used thematic analysis to identify themes and sub-themes from the qualitative data.

Results

Our case study found that Gauteng developed an innovative, multi-sectoral and comprehensive provincial COVID-19 response that aimed to address the dual challenge of saving lives and the economy. However, the interviews revealed multiple perspectives, experiences, contestations and contradictions in the pandemic response. The COVID-19 pandemic exposed and amplified the fragilities of existing systems, reflected in the corruption on personal protective equipment, poor data quality and inappropriate decisions on self-standing field hospitals. Rooted in a chronic under-investment and insufficient focus on the health workforce, the response failed to take into account or deal with their fears, and to incorporate strategies for psychosocial support, and safe working environments. The single-minded focus on COVID-19 exacerbated these fragilities, resulting in a de facto health system lockdown and reported collateral damage. The key informants identified missed opportunities to invest in primary health care, partner with communities and to include the private health sector in the pandemic response.

Conclusion

Gauteng province should build on the innovations of the multi-sectoral response to the COVID-19 pandemic, while addressing the contested areas and health system fragilities.
Characteristics of opioid prescribing to outpatients with chronic liver diseases: A call for action
Authors: Agbalajobi Olufunso M. , Gmelin Theresa , Moon Andrew M. , Alexandre Wheytnie , Zhang Grace , Gellad Walid F. , Jonassaint Naudia , Rogal Shari S.
Published: December 2021

Background

Chronic liver disease (CLD) is among the strongest risk factors for adverse prescription opioid-related events. Yet, the current prevalence and factors associated with high-risk opioid prescribing in patients with chronic liver disease (CLD) remain unclear, making it challenging to address opioid safety in this population. Therefore, we aimed to characterize opioid prescribing patterns among patients with CLD.

Methods

This retrospective cohort study included patients with CLD identified at a single medical center and followed for one year from 10/1/2015-9/30/2016. Multivariable, multinomial regression was used identify the patient characteristics, including demographics, medical conditions, and liver-related factors, that were associated with opioid prescriptions and high-risk prescriptions (≥90mg morphine equivalents per day [MME/day] or co-prescribed with benzodiazepines).

Results

Nearly half (47%) of 12,425 patients with CLD were prescribed opioids over a one-year period, with 17% of these receiving high-risk prescriptions. The baseline factors significantly associated with high-risk opioid prescriptions included female gender (adjusted incident rate ratio, AIRR = 1.32, 95% CI = 1.14–1.53), Medicaid insurance (AIRR = 1.68, 95% CI = 1.36–2.06), cirrhosis (AIRR = 1.22, 95% CI = 1.04–1.43) and baseline chronic pain (AIRR = 3.40, 95% CI = 2.94–4.01), depression (AIRR = 1.93, 95% CI = 1.60–2.32), anxiety (AIRR = 1.84, 95% CI = 1.53–2.22), substance use disorder (AIRR = 2.16, 95% CI = 1.67–2.79), and Charlson comorbidity score (AIRR = 1.27, 95% CI = 1.22–1.32). Non-alcoholic fatty liver disease was associated with decreased high-risk opioid prescriptions (AIRR = 0.56, 95% CI = 0.47–0.66).

Conclusion

Opioid medications continue to be prescribed to nearly half of patients with CLD, despite efforts to curtail opioid prescribing due to known adverse events in this population.
The mitochondrial copper chaperone COX11 has an additional role in cellular redox homeostasis
Authors: Radin Ivan , Kost Luise , Gey Uta , Steinebrunner Iris , Rödel Gerhard
Published: December 2021

Mitochondria are sites of cellular respiration, which is accompanied by the generation of dangerous reactive oxygen species (ROS). Cells have multiple mechanisms to mitigate the dangers of ROS. Here we investigate the involvement of the COX complex assembly chaperone COX11 (cytochrome c oxidase 11) in cellular redox homeostasis, using homologs from the flowering plant Arabidopsis thaliana (AtCOX11) and yeast Saccharomyces cerevisiae (ScCOX11). We found that AtCOX11 is upregulated in Arabidopsis seedlings in response to various oxidative stresses, suggesting a defensive role. In line with this, the overexpression of either AtCOX11 or ScCOX11 reduced ROS levels in yeast cells exposed to the oxidative stressor paraquat. Under normal growth conditions, both Arabidopsis and yeast COX11 overexpressing cells had the same ROS levels as the corresponding WT. In contrast, the COX11 knock-down and knock-out in Arabidopsis and yeast, respectively, significantly reduced ROS levels. In yeast cells, the ScCOX11 appears to be functionally redundant with superoxide dismutase 1 (ScSOD1), a superoxide detoxifying enzyme. The ΔSccox11ΔScsod1 mutants had dramatically reduced growth on paraquat, compared with the WT or single mutants. This growth retardation does not seem to be linked to the status of the COX complex and cellular respiration. Overexpression of putatively soluble COX11 variants substantially improved the resistance of yeast cells to the ROS inducer menadione. This shows that COX11 proteins can provide antioxidative protection likely independently from their COX assembly function. The conserved Cys219 (in AtCOX11) and Cys208 (in ScCOX11) are important for this function. Altogether, these results suggest that COX11 homologs, in addition to participating in COX complex assembly, have a distinct and evolutionary conserved role in protecting cells during heightened oxidative stress.
Oncologists’ reflections on patient rights and access to compassionate use drugs: A qualitative interview study from an academic cancer center
Authors: Stout Jeremiah , Smith Cambray , Buckner Jan , Adjei Alex A. , Wentworth Mark , Tilburt Jon C. , Master Zubin
Published: December 2021

The U.S. Food and Drug Administration (FDA) allows patients with serious illnesses to access investigational drugs for “compassionate use” outside of clinical trials through expanded access (EA) Programs. The federal Right-to-Try Act created an additional pathway for non-trial access to experimental drugs without institutional review board or FDA approval. This removal of oversight amplifies the responsibility of physicians, but little is known about the role of practicing physicians in non-trial access to investigational drugs. We undertook semi-structured interviews to capture the experiences and opinions of 21 oncologists all with previous EA experience at a major cancer center. We found five main themes. Participants with greater EA experience reported less difficulty accessing drugs through the myriad of administrative processes and drug company reluctance to provide investigational products while newcomers reported administrative hurdles. Oncologists outlined several rationales patients offered when seeking investigational drugs, including those with stronger health literacy and a good scientific rationale versus others who remained skeptical of conventional medicine. Participants reported that most patients had realistic expectations while some had unrealistic optimism. Given the diverse reasons patients sought investigational drugs, four factors—scientific rationale, risk-benefit ratio, functional status of the patient, and patient motivation—influenced oncologists’ decisions to request compassionate use drugs. Physicians struggled with a “right-to-try” framing of patient access to experimental drugs, noting instead their own responsibility to protect patients’ best interest in the uncertain and risky process of off-protocol access. This study highlights the willingness of oncologists at a major cancer center to pursue non-trial access to experimental treatments for patients while also shedding light on the factors they use when considering such treatment. Our data reveal discrepancies between physicians’ sense of patients’ expectations and their own internal sense of professional obligation to shepherd a safe process for patients at a vulnerable point in their care.
Characterizing and mapping the spatial variability of HIV risk among adolescent girls and young women: A cross-county analysis of population-based surveys in Eswatini, Haiti, and Mozambique
Authors: Brugh Kristen N. , Lewis Quinn , Haddad Cameron , Kumaresan Jon , Essam Timothy , Li Michelle S.
Published: December 2021

Background

To stem the HIV epidemic among adolescent girls and young women (AGYW), prevention programs must target services towards those most at risk for HIV. This paper investigates approaches to estimate HIV risk and map the spatial heterogeneity of at-risk populations in three countries: Eswatini, Haiti and Mozambique.

Methods

We analyzed HIV biomarker and risk factor data from recent population-based household surveys. We characterized risk using three approaches: complementary log-log regression, latent class analysis (LCA), and presence of at least one risk factor. We calculated the proportion and 95 percent confidence intervals of HIV-negative AGYW at risk across the three methods and employed Chi-square tests to investigate associations between risk classification and HIV status. Using geolocated survey data at enumeration clusters and high-resolution satellite imagery, we applied algorithms to predict the number and proportion of at-risk AGYW at hyperlocal levels.

Results

The any-risk approach yielded the highest proportion of at-risk and HIV-negative AGYW across five-year age bands: 26%-49% in Eswatini, 52%-67% in Haiti, and 32%-84% in Mozambique. Using LCA, between 8%-16% of AGYW in Eswatini, 37%-62% in Haiti, and 56%-80% in Mozambique belonged to a high vulnerability profile. In Haiti and Mozambique, the regression-based profile yielded the lowest estimate of at-risk AGYW. In general, AGYW characterized as “at risk” across the three methods had significantly higher odds of HIV infection. Hyperlocal maps indicated high levels of spatial heterogeneity in HIV risk prevalence and population density of at-risk AGYW within countries.

Conclusion

Characterizing risk among AGYW can help HIV prevention programs better understand the differential effect of multiple risk factors, facilitate early identification of high-risk AGYW, and design tailored interventions. Hyperlocal mapping of these at-risk populations can help program planners target prevention interventions to geographic areas with populations at greatest risk for HIV to achieve maximal impact on HIV incidence reduction.
Design and development of human resource management computer system for enterprise employees
Authors: Wang Tianqi , Li Ning , Li Houran
Published: December 2021

Client-Server (C/S) application is always used in the existing Human Resource Management System (HRMS) as the system architecture, which has the problems of complex maintenance and poor compatibility; and cannot use professional database and development system, making the system development difficult and the data security low. To solve the above problems, the overall demand is analyzed, as well as feasibility and key technologies of the enterprise HRMS system. Then a HRMS is designed and developed, based on the user’s key functional requirements and related technologies, which is reasonable and easy to maintain. The system is supported by Browser-Server (B/S) structure, with the current popular Java 2 Platform Enterprise Edition (J2EE) multi-level structure as the overall architecture. The mature Microsoft SQL Server 2008 introduced by Microsoft is used as the database platform. Combined with Model View Controller (MVC) design pattern, this system can be used by users without geographical restrictions and system maintenance. In this system, performance logic and business logic are separated, which makes it convenient for the development and maintenance of the system. The system mainly includes six modules: personnel management, organizational management, recruitment management, training management, salary management and system management, which integrates enterprise information and realizes the functions of easy access and easy query of information database. Its interface is simple, easy to understand, and easy to operate, with low investment, low cost, high safety, good performance and easy maintenance, which help to improve the work efficiency and modern management level of enterprises. In the end, the operation performance of the system is tested. The results show that the throughput of the main functional modules in the system is greater than 100 times/s when dealing with the business, and the success rate of event processing is greater than 99%. The average response time of the business end is less than 0.4 s, and the average response time of the terminal side is less than 0.5 s, which all meet the standards. System CPU occupancy rate can be basically controlled below 30%, and memory usage rate is below 30%. In summary, the system designed here has the basic functions but also to ensure good performance, suitable for enterprise personnel management, organizational management, recruitment management, training management and salary management. The design and development of this system aims to provide technical support for the service quality of enterprise human resource management business, to improve the overall efficiency, promote the pace of enterprise strategic development, and enhance the market competitiveness of enterprises.
Long-term adaptation following influenza A virus host shifts results in increased within-host viral fitness due to higher replication rates, broader dissemination within the respiratory epithelium and reduced tissue damage
Authors: Amat Julien A. R. , Patton Veronica , Chauché Caroline , Goldfarb Daniel , Crispell Joanna , Gu Quan , Coburn Alice M. , Gonzalez Gaelle , Mair Daniel , Tong Lily , Martinez-Sobrido Luis , Marshall John F. , Marchesi Francesco , Murcia Pablo R.
Published: December 2021

The mechanisms and consequences of genome evolution on viral fitness following host shifts are poorly understood. In addition, viral fitness -the ability of an organism to reproduce and survive- is multifactorial and thus difficult to quantify. Influenza A viruses (IAVs) circulate broadly among wild birds and have jumped into and become endemic in multiple mammalian hosts, including humans, pigs, dogs, seals, and horses. H3N8 equine influenza virus (EIV) is an endemic virus of horses that originated in birds and has been circulating uninterruptedly in equine populations since the early 1960s. Here, we used EIV to quantify changes in infection phenotype associated to viral fitness due to genome-wide changes acquired during long-term adaptation. We performed experimental infections of two mammalian cell lines and equine tracheal explants using the earliest H3N8 EIV isolated (A/equine/Uruguay/63 [EIV/63]), and A/equine/Ohio/2003 (EIV/2003), a monophyletic descendant of EIV/63 isolated 40 years after the emergence of H3N8 EIV. We show that EIV/2003 exhibits increased resistance to interferon, enhanced viral replication, and a more efficient cell-to-cell spread in cells and tissues. Transcriptomics analyses revealed virus-specific responses to each virus, mainly affecting host immunity and inflammation. Image analyses of infected equine respiratory explants showed that despite replicating at higher levels and spreading over larger areas of the respiratory epithelium, EIV/2003 induced milder lesions compared to EIV/63, suggesting that adaptation led to reduced tissue pathogenicity. Our results reveal previously unknown links between virus genotype and the host response to infection, providing new insights on the relationship between virus evolution and fitness.
HUMAN RESOURCES AVAILABILITY IN GLAM DEVELOPMENT AT INDONESIA INSTITUTE OF SCIENCES (LIPI)
Authors:   Indonesian Institutes Of Sciences, Kamaludin Kamaludin
Published: December 2021

The collections of galleries, libraries, archives and museums (GLAM) are still scattered in the work units at LIPI. To manage these collections, adequate human resources, both quantitative and qualitative, are needed. When this research was conducted, there was a socialization activity to collaborate the GLAM management among work units at LIPI. This study aims to determine human resources available in developing GLAM at LIPI. The research method used was qualitative research. Analysis of data in qualitative research was carried out narratively. The research results show that human resources for gallery/museum exhibition at LIPI have been managed by the Center for the Utilization and Innovation of Science and Technology (PPII)-LIPI in collaboration with third parties since 2020. There were 49 librarians (37 expert librarians and 12 skilled librarians), while the number of archivists was 141 (one hundred and forty-one) people (83 Expert Archivists and 58 Skilled Archivists). The collaboration of PPII-LIPI with third parties has carried out human resources for museum management at LIPI since 2020. To improve the quality, human resources managing GLAM at LIPI need to be continuously improved, both through formal and non-formal education. The number of librarians and archivists are sufficient, but they must improve their abilities, considering that the GLAM institution at LIPI will just be developed. Of course, this GLAM is still perceived as a new institution by librarians and archivists at LIPI. It is recommended that during the development of the GLAM, gallery and museum management can be carried out by LIPI employees who have competence in galleries and museums.
PERSPECTIVES ON KNOWLEDGE MANAGEMENT APPLICATION IN BPPT LIBRARY AS AN ORGANIZATIONAL KNOWLEDGE COLLECTION PLATFORM
Authors:   Badan Pengkajian dan Penerapan Teknologi (BPPT), Ariani Ria , Ucyari Rasty ,   Badan Pengkajian dan Penerapan Teknologi (BPPT), Rahayu Lisda ,   Badan Pengkajian dan Penerapan Teknologi (BPPT)
Published: December 2021

This study evaluates the practice of a knowledge management system through the knowledge management application used by the Agency for the Assessment and Application of Technology (BPPT). The purpose of this study is to understand and get an overview from BPPT employees related to BPPT knowledge management (KM) application in its function as an organizational knowledge collection platform. This study is hopefully beneficial to see the effectiveness of the features of KM application in supporting the work activities of BPPT employees. The method used in this research is quantitative, using a questionnaire technique based on a survey to gather the data. The questionnaire was distributed to employees who are the administrator of the KM BPPT application from each BPPT working unit. The collected data were processed and analyzed using the AIDA model. This study indicates that BPPT employees have used the BPPT KM application, and some of the features also have been used, such as chats, tasks, drives, and events. Moreover, based on the results obtained, it is known that BPPT employees trust the app and consider the BPPT KM application reliable to store and manage organizational knowledge properly.