bluuebunny/crossref_metadata_2025 huggingface dataset:
PEDAGOJİK FORMASYON SERTİFİKA PROGRAMINA KATILAN ÖĞRENCİLERİN ÖĞRETMENLİK MESLEĞİNE YÖNELİK TUTUMLARA İLİŞKİN MAKALELERİN İNCELENMESİ
Authors: TOR Dürdane , SERT Esra
Published: February 2022

Bu çalışmada, Türkiye’de pedagojik formasyon sertifikası eğitimi alan öğrencilerin öğretmenlik mesleğine ilişkin tutumlarına yönelik gerçekleştirilen çalışmaları incelemek ve sistematik bir derlemesini yapmak amaçlanmıştır. Çalışmanın örneklemini Temmuz 2010 – Haziran 2020 yılları arasında yayımlanmış, Ulakbim veri tabanında araştırmacılar tarafından belirlenmiş ölçütler aracılığıyla taranılan ve tam metinlerine ulaşılabilen 35 makale oluşturmuştur. Veriler içerik analizi ile işlenmiştir. Elde edilen bulgular grafikler ve tablolar halinde sunulmuş ve 7 başlık altında toplanmıştır. Bunlar sırasıyla; çalışmaların yayımlandığı yıllar, konular, örneklemler, araştırma desenleri, kullanılan veri toplama araçları, analiz yöntemleri ve araştırma sonuçlarıdır. Sonuç olarak farklı üniversitelerde okuyan formasyon öğrencileriyle gerçekleştirilmelerine rağmen, özellikle veri toplama yöntemi ve analizi açısından birbirini tekrarlamış çalışmaların ortaya çıkmış olduğu söylenebilir. Ayrıca formasyon öğrencileriyle yapılan araştırmaların sonuçları, eğitim fakültesi öğrencileriyle yapılan araştırma sonuçları ile çoğunlukla örtüşmektedir. Bu nedenle daha derinlemesine inceleme yapma ve bilgi elde etme için, özellikle araştırma yöntem ve tekniği açısından, çalışmaların farklılaştırılması gerekmektedir.
FACULTY MEMBERS' DIGITAL FOOTPRINT EXPERIENCES AND DIGITAL FOOTPRINT AWARENESS
Authors: KARABATAK Songül , ALANOĞLU Müslim
Published: February 2022

This study aimed to reveal the relationships between faculty members' gender and Internet usage time (daily usage time and years of use), their use of online environments, their digital footprint experiences, and digital footprint awareness. For this purpose, the relational research model was adopted in the study. The sample of the study consists of 398 faculty members working at a state university in Turkey. Descriptive and relational analyzes were made for the analysis of the data. Mean, percentage, and frequency analysis were used in descriptive analysis. For the relational analysis, the association rule, one of the data mining methods, was used. At the end of the study, the faculty members' digital footprint awareness was high, and their negative digital environment experiences were low. It was observed that the digital footprint experiences and awareness of female faculty members were significantly higher than that of male faculty members. It was found that faculty members mostly use online chat tools, social networks, e-mail services, and least blogs, learning management systems, and Wiki. Faculty members who indicated that digital media posts reflect their real thoughts stated that they do not regret these posts, and after checking their writing, they are sharing them in digital media. Finally, researchers were suggested to examine the change in digital footprint awareness according to regional and cultural differences.
Challenges Experienced by Healthcare Workers on Maternal Tetanus Toxoid Vaccination in Kilimanjaro Region Tanzania
Authors: Laizer Sweetness Naftal , Hemed Asia , Cyril Perry M. , Lintu Zuhura , Biswaro Sanjura Mandela , Mmbaga Blandina Theophil , Msoka Agnes Cyril
Published: February 2022

Background: Healthcare Workers (HCWs) are the first part of call for healthcare enquirers and have a key role in providing appropriate information and evidence-based healthcare recommendations to pregnant and postnatal mothers in order to ensure that they are well informed regarding maternal vaccinations. The World Health Organization (WHO) recommends that five doses are needed in order to acquire lifelong immunity against tetanus. A pregnant mother is considered to be more than 80% protected once she receives at least two doses of the vaccine. This study aimed to identify challenges experienced by the healthcare workers on maternal Tetanus Toxoid (TT) vaccination in Kilimanjaro region, Tanzania. Method: A qualitative approach comprising of In-Depth Interviews (IDIs) and focus group discussion (FGD) was conducted with 10 healthcare workers selected from 10 health facilities in remote villages of Hai district. The healthcare workers were selected purposively based on their availability and convenience; thematic analysis guided the data process. Results: Majority of the Healthcare workers were female, enrolled nurses with college education; their age ranged between 26-59 years with experience of work between 1 to 31 years. Main themes were extracted from the analysis namely: unreliable storage of vaccines, shortage of vaccines, shortage of healthcare workers, behavior of the healthcare workers and spousal influence on maternal vaccinations. Conclusion: The shortage and unreliable storage of TT vaccine has led to mothers missing their required doses, the spousal support and good mother-provider relationship is essential to ensure that the pregnant and post-natal mothers receive all the required doses. The regional and district health officials should ensure constant supply of TT vaccine with the recommended standards of vaccine storage facilities.
Case Report: Successful Treatment of COVID-19 ARDS with Transcutaneous Vagus Nerve Stimulation
Authors: DO Patrick Nemechek , Evlogiev Ivan
Published: February 2022

We report two individuals who successfully recovered from COVID-19-induced ARDS after being treated with transcutaneous auricular vagus nerve stimulation (taVNS). In the first case, taVNS was started after eleven hospital days of continual deterioration despite mechanical ventilation along with supportive therapy of steroids, antibiotics, and immunoglobulins. On the twelfth day of hospitalization, taVNS was begun four times daily, resulting in sudden improvement in oxygenation. There was a concomitant reduction of c-reactive protein from 109.6 μg/mL to 10 μg/mL. After nine days of taVNS therapy, the patient's improved respiratory function improved enough that she was able to be extubated. No additional treatments had been added or changed during this time. After twenty-seven days of hospitalization, the patient was discharged to home without supplemental oxygen. The second case involves taVNS therapy in the home setting. After nineteen days of progressive deterioration, the patient's oxygen saturation declined to 90% on room air before taVNS treatment was instituted. After tens days of taVNS treatment three times daily, his oxygen saturation had climbed to 97%, and by day 25 of VNS therapy, the patient felt he was fully recovered. taVNS is capable of improving regulation of the inflammatory response in other clinical conditions such as autoimmune disorders in humans and septic shock in animal models. We suggest that transcutaneous auricular vagus nerve stimulation is potent enough to improve the clinical course of COVID-19 associated ARDS because of its potential to minimize the clinical deterioration associated with the immunological surge known as the cytokine storm. taVNS is a safe and potentially efficacious treatment for COVID-19 ARDS.
Epidemiological, Clinical, Therapeutic and Evolutionary Aspects of Corneal Abscesses at the Teaching Hospital of Bouaké (Côte d’Ivoire)
Authors: Y Ouattara , Z Diabaté , LE Godé , KFH Koffi , Y Tembely , ORL Babayeju , L Sékongo , PEFK Bilé , GF Diomandé , IA Diomandé
Published: February 2022

Introduction: The purpose of this study was to describe the epidemiological, clinical, therapeutic and evolutionary aspects of corneal abscesses treated in the ophthalmology department at the Teaching Hospital of Bouaké, in order to contribute to an improvement in their management. Materials and methods: A longitudinal retrospective study based on the analysis of medical records of patients with corneal abscesses who received follow-up in the department. from 1 January 2012 to 31 December 2016 and 21 cases of unilateral abscesses were collected from a total of 18,585 cases. Results: The frequency of corneal abscesses was 0.11%. The average age of patients was 27.71 years (1-year and 73-year extremes) with a male predominance (1.1 sex ratio). Housewives and farmers accounted for 57.14%. Patients were admitted more than 7 days after the onset of signs in 33.33% of cases. At admission, the visual acuity of the affected eye was less than 1/20 in 16 patients (76.19%) and among them, 6 had no light perception. The abscess was localized to the left eye in 11 patients (52.38%). Its largest diameter was greater than 2 millimetres in 14 patients (66.66%), its seat was in the visual axis in 10 patients (47.61%) and in 12 patients (57.14%), its depth exceeded two-thirds of the corneal thickness. Corneal perforation was observed in 6 cases (28.57%) and severity factors were found in 19 patients (90.47%). No microbiological examination was performed and treatment was based on probabilistic antibiotic therapy. The cure was achieved but at the cost of anatomical sequelae whose functional consequences were visual acuity less than 1/20 in 76.19% of cases. Conclusion: corneal abscesses are infrequent but very serious with poor prognosis in our conditions. This prognosis can be improved by early and adequate management, including microbiology-guided antibiotic therapy.
Critical Study of Phenomenology through Publication about Islamic Education
Authors: Elihami Elihami
Published: February 2022

This study aims to describe the phenomenology of the level of critical thinking skills and student publication achievements, and to determine the level of the relationship between critical thinking skills and student publication achievements on students of the Muhammadiyah Se-Ajatappareng College. Data were obtained through interview instruments and documentation analysis from 12 students who were determined as samples from representatives of Muhammadiyah Se-Ajatappareng College students with purposive sampling technique. The results showed that there was an accumulation of critical thinking skills with good categories that were assessed from the results of papers that had been published locally and were correlated by students, while the achievements of student publications were shown in very low categories with search through Google scholar. The relationship between critical thinking skills and publication achievement is shown by conclusions, that there is no significant relationship between critical thinking skills and the achievement of student publications on students at the Muhammadiyah Se-Ajatappareng College. Judging from the phenomenology study shows that the tradition of publication for students is not yet a written culture but is still in the category of oral traditions / speakers.
THE ELEMENTARY STUDENTS’ CONCEPT OF ISLAMIC EDUCATION: THE IMPACT OF THE FULL DAY SCHOOL
Authors: Rahamma Tawany , Elihami Elihami , Dangnga Muhammad Siri , Ali Sitti Nurhayati
Published: February 2022

The purpose of this study is to teach and learning in the Elementary School as a Elementary School involved in education and teaching is to use full day school system to provide feedback to students through Islamic Education. The qualitative-based research methodology is characteristic evaluation involving non-participant observation, interview, and documentation. This study aims to find out how the implementation of full day school affects the level of involvement of student participation. The survey items selected for this study are authorized and the field research of activity. In the aspect of student involvement, it is generally reported to have a positive attitude involved in education and teaching is to use full day school system. Participating in full day school using the full day school is a strong positive experience for students from the Elementary School. The student's vision of the benefits of full day school learning through Islamic education was very good. The students as prospective teachers in the sample as a whole tend to assess program of the government the use of student response systems in improving learning
PERAN FIQIH DAN PRINSIP IBADAH DALAM ISLAM
Authors: Nur Indah Arnilah
Published: February 2022

Penelitian ini merupakan penelitian kepustakaan dan bersifat deskriptif analitik. Fokus penelitian diarahkan untuk mengkaji Fiqih Ibadah dan Prinsip Ibadah dalam Islam. Penelitian ini menyimpulkan bahwa;Islam menegakkan ibadah atas beberapa sendi yang dapat membersihkan jiwa dan tetap memelihara inti sari ibadah dan setiap ibadah memiliki hikmah. Fiqih Ibadah adalah pemahaman terhadap yang berkaitan dengan peribadahan manusia kepada Allah SWT. ditujukan sebagai rasa bentuk ketundukan dan harapan untuk mecapai ridla Allah SWT.
MUHAMMADIYAH TAJDID MOVEMENT IN INDUSTRIAL ERA 4.0
Authors: Sari Nur Indah , Elihami Elihami
Published: February 2022

The challenges faced by Muhammadiyah in the Industrial 4.0 era were strengthening economic, Islamic, cultural and political integration, and various other elements even though the continuity between several components was not optimal. Without Muhammadiyah's renewal efforts in the Industrial 4.0 era which included a strategy of renewing the education movement that it has been engaged in and various other movements, recognizing contemporary methods and approaches to Islamic and Islamic studies and other variants, being inclusive towards the development of experience and knowledge of the younger generation, open dialogue between culture and religion at the grassroots, and so on.
TEACHER'S EFFORTS TO IMPLEMENT ONLINE LEARNING ACTIVITIES IN TRAINING THE INDEPENDENCE OF CHILDREN IN CHILDREN IN THE ERA OF VIRUS COVID-19
Authors: Elihami Elihami , Syafitra Noer
Published: February 2022

Research on teachers' efforts to carry out online learning activities in training the independence of early childhood in TKI PGRI Standard Corawali in the covid -19 era, of course, there are many problems including: Inhibiting factors, over-protective and over-parent factors in educating children when learning online conducted by the teacher. Environmental factors often have an adverse effect on children because they hold more gadgets. Internal factors of children who have abnormalities from birth. Media factor. television and games have a profound effect on children, especially in negative matters. Children prefer to imitate movie scenes rather than imitate what teachers and parents teach. Supporting factors, curriculum in accordance with the needs and development of children, children do not feel burdened by learning. All learning is related to perminan so that children are always cheerful and happy. The number of teachers is balanced so it is easy to pay attention and control their students. Complete facilities and infrastructures become one of the supporting children in learning, children easily choose play equipment according to their talents and interests assigned online.
Response of Muslim Prayer to COVID-19 in Indonesia: The Role of Muhammadiyah Mosque in Enrekang, South Sulawesi
Authors: M Dyah Mutmainna Muinuddin , Elihami Elihami
Published: February 2022

Abstract

This study discusses how the prayer in the mosque when the world is in a precarious situation caused by the Covid-19 virus originating from the city of Wuhan, China. Several UN countries including Indonesia have been infected by the virus. Indonesia itself has done various ways of handling related to this virus, one of which is to conduct PSBB or large-scale social restrictions that have been implemented in various major cities throughout Indonesia. The Indonesian government has also announced not to perform worship activities at the mosque or in other words pray at their respective homes. The results of this study indicate the role of Muhammadiyah Mosque in dealing with the problem of congregational prayers in the midst of covid-19. The Muhammadiyah Mosque made a decision to assist the government in breaking the chain of the spread covid-19 outbreak by not using or closing the mosque to pray in congregation.
Analysis of the need for development of e-module based on interactive media learning in the era of the pandemic
Authors: Setiawan Dwi Candra , Ni’mah Fauziatun , Karolina Wiwin
Published: February 2022

The COVID-19 pandemic has had a big impact on almost all sectors, including the education sector. What felt from this outbreak is the learning process from offline to online. The change in the learning process requires educators to be creative in developing learning resources, especially online learning resources to facilitate the learning. The purpose of this study was to obtain initial data as a requirement for the development of interactive media-based e-modules. The research that will be conducting is descriptive qualitative. The population used is students of SMP/MTs in Malang, with a sample of 30 students of class IX in MTs. Sunan Ampel Poncokusumo, 30 Class IX students at SMPI Nurul Hikmah Assalafiyah Poncokusumo, 15 SMP Sunan Kalijogo Jabung Class IX students at and 15 Class IX students at SMP Plus Fityani Pujon. The instruments used are observation sheets, questionnaires and interview sheets. Data collection techniques were carried out using observation, distributing questionnaires and interviews. Data were analyzed using descriptive analysis. The results of the needs analysis test show that online-based teaching materials are needed to support the learning process in the era of the covid-19 pandemic, where 96.67% of students need an online-based teaching material and 100% of the results of teacher interviews need to develop online-based teaching materials. Based on the results of the research and discussion, it can be concluded that there is a need for the development of interactive media-based e-modules as a learning solution in the pandemic era. The results of this need analysis test can be used as basic data for the development of interactive media-based e-modules.
‘Circles of Empathy’: Edinburgh International Festivals Diary 2021
Authors: Relich Mario
Published: February 2022

A personal review of the 2021 Edinburgh International Festivals, which made limited but welcome returns to ‘in-person’ events in the wake of the COVID pandemic.
Our Plural Gàidhealtachd: An Editorial Rejoinder
Authors: Oliver James , MacKinnon Iain
Published: February 2022

In the preamble of the response articles to the recent special issue of Scottish Affairs on Scotland's Gàidhealtachd Futures the authors make some negative assertions about the editorial position. This short and positive essay revisits and reiterates the key themes of ‘plurality’ and ‘futures’ that are the focus of the special issue's contribution to important discussions and emerging priorities for Scotland's Gàidhealtachd Futures.
Including the Threatened First-language Gaelic Vernacular Community in Gaelic Promotion and Protection: A Rebuttal to McLeod et al.
Authors: Ó Giollagáin Conchúr , Caimbeul Iain , Ó Curnáin Brian , Camshron Gòrdan , Moireach Pàdruig
Published: February 2022

This article serves as a rebuttal to McLeod et al’s criticism of Ó Giollagáin and Caimbeul’s ‘Moving Beyond Asocial Minority Language Policy’ article in this journal, while also offering an analysis of McLeod et al’s disapproving viewpoint of the conclusions and recommendations in Ó Giollagáin et al’s The Gaelic Crisis in the Vernacular Community (2020). This rebuttal argues for the inclusion of the threatened first-language Gaelic vernacular community in Gaelic policy, as well as the integration of the protection of Gaelic communities in official Gaelic promotion – minority language promotion with language community protection. McLeod et al’s contribution to the debate on the Gaelic vernacular crisis is essentially a collective effort to reinforce the relegation of the Gaelic crisis within official language promotion in Scotland and to promote a reinvigorated status quo.
How to Conference
Authors: Turner Kimberly , Boyes Christina , Bennion Elizabeth , Newman James
Published: February 2022

This chapter provides advice regarding strategies and approaches to attending academic conferences. The chapter addresses networking, presenting your research, which conferences to attend and why. The chapter provides specific advice from the perspective of recently finished graduate students and seasoned professors. “This manuscript is part of Strategies for Navigating Graduate School and Beyond, a forthcoming volume for those interested in pursuing graduate education in political science (Fall 2022 publication).”
BAHAYA DAN DAMPAK PENYALAHGUNAAN NAPZA DI KALANGAN PELAJAR SMAN 9 BEKASI
Authors: Al Adawiah Rabiah
Published: February 2022

Angka penyalahgunaan NAPZA/Narkotika di Indonesia mencapai 3.419.188 orang, dan 27% pengguna NAPZA tersebut dari kalangan pelajar. Besarnya angka ini menjadi masalah serius terutama bagi generasi muda. Penyalahgunaan NAPZA dapat membahayakan dan memberikan dampak yang serius terhadap fisik, psikis, bahkan dampak sosial bagi penggunanya. Dengan demikian menjadi sangat penting bagi semua pihak baik sekolah maupun orang tua untuk terus memberikan pengarahan yang baik mengenai bahaya penyalahgunaan NAPZA/Narkotika bagi masa depan pelajar.

Secara umum, tujuan dari kegiatan pengabdian kepada masyarakat berupa penyuluhan hukum penyalahgunaan Napza ini, yaitu: 1) Pihak sekolah turut mencegah terjadinya penyalahgunaan Napza di kalangan pelajar; 2) Kalangan pelajar dapat mengerti dan memahami bahaya dan dampak penyalahgunaan Napza dan aspek hukumnya. Metode kegiatan dilaksanakan dalam bentuk webinar melalui platform/aplikasi zoom antara narasumber dengan para peserta pelajar.
PENDIRIAN KOPERASI SIMPAN PINJAM PERUMAHAN REXVIN BOULEVARD BATAM
Authors: Mardika Nanda harry
Published: February 2022

Permasalahan keuangan sering kali menjadi permasalahan utama pada masyarakat. Kebutuhan hidup yang cukup tinggi dan kurangnya  modal usaha menjadi sub faktor dari permasalahan tersebut. Hal ini juga sangat dirasakan oleh warga rexvin boulevard, kelurahan tembesi, kecamatan sagulung, kota batam. Untuk memenuhi kebutuhan pembiayaan usaha dan penyimpanan, masyarakat membutuhkan suatu lembaga keuangan yang bisa menjadi solusi untuk memperbaiki dan mengembangkan kondisi perekonomian masyarakat yang belum stabil yang disebabkan berbagai macam beban pengeluaran harian dan bulanan yang ditanggung, modal usaha yang terbatas serta pengeluaran tak terduga. Berdasarkan hal-hal tersebut, pengabdi menginisiasi rencana pendirian koperasi simpan pinjam bersama masyarakat setempat di perumahan Rexvin Boulevard. Pengabdian ini di aplikasikan dengan memaparkan dan menjelaskan bagaimana membentuk koperasi simpan pinjam, mengelola koperasi dan mengembangkan sumberdaya manusia di perumahan rexvin boulevard, tembesi, sagulung – kota batam.
Pembinaan Pengendalian Manajemen Keuangan Dengan Aplikasi ABSS Accounting Pada UKM Bank Sampah Mandiri
Authors: Effendi Syahril , Tukino Tukino , Maulana Algifanri
Published: February 2022

Berdasarkan hasil wawancara permasalahan Perusahaan-perusahaan kecil atau menengah yang tidak menggunakan software akuntansi untuk mencatat pembukuannya. Cara tradisional pencatatan keuangan secara manual masih sering digunakan karena kurangnya sumber daya manusia yang memiliki keahlian atau skill dibidang akuntansi serta memakan biaya untuk pembelian peralatan komputer dan software pada sistem komputerisasi pada badan-badan usaha kecil tersebut. UKM perlu dilatih membuat pembukuan, sehingga nantinya bisa memisahkan bagian modal yang digunakan untuk operasional usaha dan bagian uang yang dapat digunakan untuk keperluan sehari-hari. Metode yang digunakan dalam pembinaan UKM Bank Sampah Mandiri di Sagulung - Kota Batam Kota Batam yang akan diberikan yaitu metode survei, metode ceramah, metode diskusi dan metode latihan. Keberlanjutan dari hasil kegiatan pembinaan yaitu diharapkan UKM Bank Sampah Mandiri di Sagulung - Kota Batam mampu memahami pencatatan serta tujuan dalam catatan laporan keuangan, mampu menganalisis dan membuat laporan keuangan secara mandiri sesuai dengan siklus akuntansi setiap periodenya, mampu menyusun laporan keuangan dengan bantuan program ABSS Accounting, dapat meningkatkan kinerja keuangan UKM Bank Sampah Mandiri di Sagulung - Kota Batam.
Pelatihan Peningkatan Ekonomi Masyarakat Desa Galang Baru Melalui Eco Wisata Bahari
Authors: Raymond Raymond , Putri Anggia Dasa , Siregar Dian Lestrari
Published: February 2022

Pulau Batam memiliki wilayah pesisir yang memiliki keindahan dan potensi besar dalam pengembangan pariwisata. Namun, perkembangan pariwisata di kota Batam memunculkan permasalahan baru yaitu pembangunan pariwisata tidak lagi memperhatikan kondisi lingkungan yang selaras dengan pembangunan ekonomi. Padahal sebagai tujuan destinasi wisata kota Batam adalah mengedepankan potensi alamnya yang indah berupa pantai dan keindahan laut. Salah satu dari banyak wilayah di Kota Batam yang memiliki potensi dalam pengembangan wisata bahari adalah wilayah pesisir Desa Galang Baru yang mempunyai potensi dan daya Tarik wisata khususnya wisata di bidang kemaritiman, seperti wisata pantai di tanjung cakang, Pulau sembur, Pulau korek, terumbu karang di pulau sembur dan pulau nguan, Hutan bakau di Tanjung Caking dan Pulau Nguan, serta makanan laut (seafood) di Galang Baru, Pulau Korek, Tanjung Cakang. Namun dibalik potensi wisata bahari di galang baru ternyata tidak dimanfaatkan secara optimal, untuk itu fokus program pengembangan desa mitra kali ini adalah pada empat aspek seperti pengembangan destinasi wisata, pengembangan Pemasaran pariwisata, pembentukan Industri pariwisata, serta pembentukan Kelembagaan pariwisata. hasil dari program ini yaitu memberikan pengetahuan bagi masyarakat galang baru tentang eco Wisata bahari unggulan
BSImp: Imputing Partially Observed Methylation Patterns for Evaluating Methylation Heterogeneity
Authors: Chang Ya-Ting Sabrina , Yen Ming-Ren , Chen Pao-Yang
Published: February 2022

DNA methylation is one of the most studied epigenetic modifications that has applications ranging from transcriptional regulation to aging, and can be assessed by bisulfite sequencing (BS-seq) or enzymatic methyl sequencing (EM-seq) at single base-pair resolution. The permutations of methylation statuses given by aligned reads reflect the methylation patterns of individual cells. These patterns at specific genomic locations are sought to be indicative of cellular heterogeneity within a cellular population, which are predictive of developments and diseases; therefore, methylation heterogeneity has potentials in early detection of these changes. Computational methods have been developed to assess methylation heterogeneity using methylation patterns formed by four consecutive CpGs, but the nature of shotgun sequencing often give partially observed patterns, which makes very limited data available for downstream analysis. While many programs are developed to impute genome-wide methylation levels, currently there is only one method developed for recovering partially observed methylation patterns; however, the program needs lots of data to train and cannot be used directly; therefore, we developed a probabilistic-based imputation method that uses information from neighbouring sites to recover partially observed methylation patterns speedily. It is demonstrated to allow for the evaluation of methylation heterogeneity at 15% more regions genome-wide with high accuracy for data with moderate depth. To make it more user-friendly we also provide a computational pipeline for genome-screening, which can be used in both evaluating methylation levels and profiling methylation patterns genomewide for all cytosine contexts, which is the first of its kind. Our method allows for accurate estimation of methylation levels and makes evaluating methylation heterogeneity available for much more data with reasonable coverage, which has important implications in using methylation heterogeneity for monitoring changes within the cellular populations that were impossible to detect for the assessment of development and diseases.
Strontium Ion Removal From Artificial Seawater Using a Combination of Adsorption With Biochar and Precipitation by Blowing CO2 Nanobubble With Neutralization
Authors: Guo Yixuan , Hong Nhung Nguyen Thi , Dai Xiang , He Chunlin , Wang Youbin , Wei Yuezhou , Fujita Toyohisa
Published: February 2022

While enjoying the convenience of nuclear energy development, the environmental contamination by radionuclide leakage is of significant concern. Because of its cost-effectiveness and environmental friendliness, biochar has attracted a lot of attention in the field of radioactive water treatment. Herein, a novel teak peel modified biochar (labeled as PMBN3) was prepared and applied to remove strontium from artificial seawater. The characterisation of the prepared PMBN3 showed it contains numerous oxygen-containing functional groups (i.e. carboxyl and hydroxyl groups), laminar morphology, mesoporous structure, large specific surface area. PMBN3 exhibited great advantages in Sr(II) adsorption, such as rapid adsorption kinetics (<1 h for equilibrium) and superior reusability. The adsorption of strontium by biochar is consistent with pseudo-second order and internal diffusion kinetic models. Among the four types of adsorption isotherms, the Freundlich isotherm showed the best fit with R2 > 0.98. The calculated thermodynamic parameters indicate that strontium adsorption on biochar occurs exothermically and spontaneously. Furthermore, for efficient removal of Sr(II), CO2 nanobubbles were blown into artificial seawater to precipitate the interfering metal ions, and followed by the adsorption of PMBN3 towards residual metal ions with the removal rate of Sr(II) over 99.7%. Finally, mechanistic studies have shown that the strontium adsorption process by PMBN3 is a multiple adsorption mechanism consisting of ion exchange between H+ (from -OH and -COOH) and Sr(II), and weak intermolecular forces between Sr(II) and the PMBN3 adsorbent. This study creatively combines chemisorption and nanobubble precipitation for strontium removal, which provides great reference value and guidance for environmental remediation.
Impact of Construction Delay-Controlling Parameters on Project Schedule: DEMATEL-System Dynamics Modeling Approach
Authors: Ajayi Babatunde Oluwaseun , Chinda Thanwadee
Published: February 2022

Project delay has been a phenomenon contending with the construction sector opposing time, cost, and quality. This study develops a model, which is a combination of two mathematical decision-making methods DEMATEL and system dynamics modeling, to investigate the impact of the factors on the entire project schedule. The simulation results reveal the importance of minimizing design error to reduce the project delay at the beginning (or preconstruction stage) of the project. Rework, design changes, and change orders must also be closely monitored during the construction to ensure work productivity and on-time project completion. The results also reveal that experienced designers and the use of updated design technology help reduce design errors, thus enhancing work performance in the long term. As shown in the simulation results, the actual project completion time and magnitude of delay steadily reduce with the project series. This explains the fact that the more the consistency and effectiveness in handling the delay factors as similar projects are performed in turns, the better the project performance in terms of time. Therefore, careful consideration of the delay factors reduces the time lag and delay magnitude in construction projects. Other improvements, such as effective supervision during construction which is a function of experienced supervisors, good project management, and cooperation among key stakeholders, including owners, consultants, and contractors, also assist in reducing the construction delay in the long term.
Exosomes Immunity Strategy: A Novel Approach for Ameliorating Intervertebral Disc Degeneration
Authors: Li Weihang , Zhang Shilei , Wang Dong , Zhang Huan , Shi Quan , Zhang Yuyuan , Wang Mo , Ding Ziyi , Xu Songjie , Gao Bo , Yan Ming
Published: February 2022

Low back pain (LBP), which is one of the most severe medical and social problems globally, has affected nearly 80% of the population worldwide, and intervertebral disc degeneration (IDD) is a common musculoskeletal disorder that happens to be the primary trigger of LBP. The pathology of IDD is based on the impaired homeostasis of catabolism and anabolism in the extracellular matrix (ECM), uncontrolled activation of immunologic cascades, dysfunction, and loss of nucleus pulposus (NP) cells in addition to dynamic cellular and biochemical alterations in the microenvironment of intervertebral disc (IVD). Currently, the main therapeutic approach regarding IDD is surgical intervention, but it could not considerably cure IDD. Exosomes, extracellular vesicles with a diameter of 30–150 nm, are secreted by various kinds of cell types like stem cells, tumor cells, immune cells, and endothelial cells; the lipid bilayer of the exosomes protects them from ribonuclease degradation and helps improve their biological efficiency in recipient cells. Increasing lines of evidence have reported the promising applications of exosomes in immunological diseases, and regarded exosomes as a potential therapeutic source for IDD. This review focuses on clarifying novel therapies based on exosomes derived from different cell sources and the essential roles of exosomes in regulating IDD, especially the immunologic strategy.
A Ferroptosis-Related Gene Prognostic Index Associated With Biochemical Recurrence and Radiation Resistance for Patients With Prostate Cancer Undergoing Radical Radiotherapy
Authors: Feng Dechao , Shi Xu , Xiong Qiao , Zhang Facai , Li Dengxiong , Wei Wuran , Yang Lu
Published: February 2022

Background: Ferroptosis is a new type of programmed cell death which has been reported to be involved in the development of various cancers. In this study, we attempted to explore the possible links between ferroptosis and prostate cancer (PCa), and a novel ferroptosis-related gene prognostic index (FGPI) was constructed to predict biochemical recurrence (BCR) and radiation resistance for PCa patients undergoing radical radiotherapy (RRT). Moreover, the tumor immune microenvironment (TME) of PCa was analyzed.

Methods: We merged four GEO datasets by removing batch effects. All analyses were conducted with R version 3.6.3 and its suitable packages. Cytoscape 3.8.2 was used to establish a network of transcriptional factor and competing endogenous RNA.

Results: We established the FGPI based on ACSL3 and EPAS1. We observed that FGPI was an independent risk factor of BCR for PCa patients (HR: 3.03; 95% CI: 1.68–5.48), consistent with the result of internal validation (HR: 3.44; 95% CI: 1.68–7.05). Furthermore, FGPI showed high ability to identify radiation resistance (AUC: 0.963; 95% CI: 0.882–1.00). LncRNA PART1 was significantly associated with BCR and might modulate the mRNA expression of EPAS1 and ACSL3 through interactions with 60 miRNAs. Gene set enrichment analysis indicated that FGPI was enriched in epithelial–mesenchymal transition, allograft rejection, TGF beta signaling pathway, and ECM receptor interaction. Immune checkpoint and m6A analyses showed that PD-L2, CD96, and METTL14 were differentially expressed between BCR and no BCR groups, among which CD96 was significantly associated with BCR-free survival (HR: 1.79; 95% CI: 1.06–3.03). We observed that cancer-related fibroblasts (CAFs), macrophages, stromal score, immune score, estimate score, and tumor purity were differentially expressed between BCR and no BCR groups and closely related to BCR-free survival (HRs were 2.17, 1.79, 2.20, 1.93, 1.92, and 0.52 for cancer-related fibroblasts, macrophages, stromal score, immune score, estimate score, and tumor purity, respectively). Moreover, cancer-related fibroblasts (coefficient: 0.20), stromal score (coefficient: 0.14), immune score (coefficient: 0.14), estimate score (coefficient: 0.15), and tumor purity (coefficient: −0.15) were significantly related to FGPI, among which higher positive correlation between cancer-related fibroblasts and FGPI was observed.

Conclusion: We found that FGPI based on ACSL3 and EPAS1 might be used to predict BCR and radiation resistance for PCa patients. CD96 and PD-L2 might be a possible target for drug action. Besides, we highlighted the importance of immune evasion in the process of BCR.
Links Between N6-Methyladenosine and Tumor Microenvironments in Colorectal Cancer
Authors: Zhang Yundi , Zhang Ke , Gong Haoming , Li Qin , Man Lajie , Jin Qingchang , Zhang Lin , Li Song
Published: February 2022

N6-methyladenosine (m6A) is a critical epigenetic modification for tumor malignancies, but its role in regulating the tumor microenvironments (TMEs) has not been fully studied. By integrating multiple data sets and multi-omics data, we comprehensively evaluated the m6A “writers,” “erasers,” and “readers” in colorectal cancer and their association with TME characteristics. The m6A regulator genes showed specific patterns in co-mutation, copy number variation, and expression. Based on the transcriptomic data of the m6A regulators and their correlated genes, two types of subtyping systems, m6AregCluster and m6AsigCluster, were developed. The clusters were distinct in pathways (metabolism/inflammation/extracellular matrix and interaction), immune phenotypes (immune-excluded/immune-inflamed/immune-suppressive), TME cell composition (lack immune and stromal cells/activated immune cells/stromal and immune-suppressive cells), stroma activities, and survival outcomes. We also established an m6Ascore associated with molecular subgroups, microsatellite instability, DNA repair status, mutation burdens, and survival and predicted immunotherapy outcomes. In conclusion, our work revealed a close association between m6A modification and TME formation. Evaluating m6A in cancer has helped us comprehend the TME status, and targeting m6A in tumor cells might help modulate the TME and improve tumor therapy and immunotherapy.
Histone 3 Methyltransferases Alter Melanoma Initiation and Progression Through Discrete Mechanisms
Authors: DiNapoli Sara E. , Martinez-McFaline Raúl , Shen Hao , Doane Ashley S. , Perez Alexendar R. , Verma Akanksha , Simon Amanda , Nelson Isabel , Balgobin Courtney A. , Bourque Caitlin T. , Yao Jun , Raman Renuka , Béguelin Wendy , Zippin Jonathan H. , Elemento Olivier , Melnick Ari M. , Houvras Yariv
Published: February 2022

Perturbations to the epigenome are known drivers of tumorigenesis. In melanoma, alterations in histone methyltransferases that catalyze methylation at histone 3 lysine 9 and histone 3 lysine 27—two sites of critical post-translational modification—have been reported. To study the function of these methyltransferases in melanoma, we engineered melanocytes to express histone 3 lysine-to-methionine mutations at lysine 9 and lysine 27, which are known to inhibit the activity of histone methyltransferases, in a zebrafish melanoma model. Using this system, we found that loss of histone 3 lysine 9 methylation dramatically suppressed melanoma formation and that inhibition of histone 3 lysine 9 methyltransferases in human melanoma cells increased innate immune response signatures. In contrast, loss of histone 3 lysine 27 methylation significantly accelerated melanoma formation. We identified FOXD1 as a top target of PRC2 that is silenced in melanocytes and found that aberrant overexpression of FOXD1 accelerated melanoma onset. Collectively, these data demonstrate how histone 3 lysine-to-methionine mutations can be used to uncover critical roles for methyltransferases.
Ferroptosis-Related IncRNAs Are Prognostic Biomarker of Overall Survival in Pancreatic Cancer Patients
Authors: Chen Dongjie , Gao Wenzhe , Zang Longjun , Zhang Xianlin , Li Zheng , Zhu Hongwei , Yu Xiao
Published: February 2022

Pancreatic cancer (PC) is one of the most lethal malignancies, the mortality and morbidity of which have been increasing over the past decade. Ferroptosis, a newly identified iron-dependent cell death pattern, can be induced by iron chelators and small lipophilic antioxidants. Nonetheless, the prognostic significance of ferroptosis-related lncRNAs in PC remains to be clarified. We obtained the lncRNA expression matrix and clinicopathological information of PC patients from The Cancer Genome Atlas (TCGA) and the International Cancer Genome Consortium (ICGC) datasets in the current study. Firstly, we conducted Pearson correlation analysis to delve into the ferroptosis-related lncRNAs, and univariate Cox analysis was implemented to examine the prognostic values in PC patients. Twenty-three prognostic ferroptosis-related lncRNAs were confirmed and loaded into the least absolute shrinkage and selection operator Cox (LASSO-Cox) analysis, then a ferroptosis-related lncRNA prognostic marker (Fe-LPM) was established in the TCGA dataset. Risk scores of patients were calculated and segregated PC patients into low-risk and high-risk subgroups in each dataset. The prognostic capability of Fe-LPM was also confirmed in the ICGC dataset. Gene set enrichment analysis (GSEA) revealed that several ferroptosis-related pathways were enriched in low-risk subgroups. Furthermore, we adopted a multivariate Cox regression to establish a nomogram based on risk score, age, pathological T stage and primary therapy outcome. A competing endogenous RNA (ceRNA) network was also created relied on four of the twenty-three ferroptosis-related lncRNAs. In conclusion, the eight Fe-LPM can be utilized to anticipate the overall survival (OS) of PC patients, which are meaningful to guiding clinical strategies in PC.
Experimental Investigation of a Continuous Reactor for CO2 Capture and CaCO3 Precipitation
Authors: Tiefenthaler Johannes , Mazzotti Marco
Published: February 2022

In a climate neutral world, the life cycle greenhouse gas (GHG) emissions of precipitated calcium carbonate (PCC) have to be reduced towards net-zero. Mineral carbonation processes allow to do so by replacing the carbon rich calcium source limestone by carbon free industrial mineral wastes. Various processes have been investigated in literature. They exhibit the benefit of little to no feedstock related emissions and high energy savings due to the avoidance of the CaCO3 calcination step. However, the nature of the process changes significantly, which requires a fundamental understanding of the new mechanisms controlling the process of CO2 absorption and CaCO3 precipitation. Within this work, a CO2 rich gas is contacted with a calcium rich aqueous feed in a continuous reactive crystallizor. The CO2 selectively absorbs and precipitates as either vaterite or calcite. The effect of the liquid and gas feed flow rates, of the feed stoichiometric ratio and of the residence time on key performance indicators, such as the CO2 capture efficiency the CaCO3 precipitation efficiency and the features of the final product, is studied experimentally. As expected, these feed characteristics determine the effective stoichiometric ratio of reactants in the liquid phase, ψ.  The particle size increases strongly with ψ; vaterite represents the predominant solid phase at ψ < 1 while otherwise a mix of vaterite and calcite was formed, whereas the latter one accounted for 13%–90% in mass of crystals collected. Moreover, ψ of about one exhibits the highest CO2 capture efficiency exceeding 80%.
Conceptual Process Design of an Integrated Xylitol Biorefinery With Value-Added Co-Products
Authors: Vollmer Nikolaus I. , Gernaey Krist V. , Sin Gürkan
Published: February 2022

This manuscript describes the conceptual process design of an integrated xylitol biorefinery with value-added co-products. Based on an existing three-step framework, the main product of a second-generation integrated biorefinery is chosen in the first stage. Based upon this, other decisions as the feedstock and value-added co-products are made. All relevant unit operations for the process are introduced. An initial superstructure with all potential process alternatives is composed of all introduced models. In the second step of the framework, a global sensitivity analysis is performed, firstly with coarse sampling to determine all viable flowsheet options and secondly with fine sampling to determine the most sensitive operational variables. As a result of the sensitivity analysis, most of the flowsheet options in the initial superstructure are not feasible. Based on these results, flowsheet sampling with the five most sensitive operational variables is performed to create surrogate models. In the scope of this work, three types of surrogate models are benchmarked against each other. Regarding the results of the superstructure optimization, firstly, it becomes apparent that the production of biokerosene does not contribute significantly to the net present value of the biorefinery. Furthermore, reducing the number of unit operations in the downstream processing leads to lower capital expenditures, but it lowers the product yield. Lastly, most flowsheets are economically feasible, indicated by a positive net present value. Based on this result, the most promising candidate process topology is subjected to the third step of the framework, including uncertainty in capital expenditure and operational expenses according to their estimations and uncertainties in the product prices. As a result, the net present value of the flowsheet turns negative, indicating that the high uncertainties for the expenditure and the expenses do not allow for an economically feasible operation. Lastly, the analysis of conceptually designed process flowsheets based on Monte Carlo sampling shows failure rates, with the NPV falling below the break-even point, of around 60% probability or higher. Based on these results, an economically feasible construction and operation of a xylitol biorefinery seems unlikely. Further ways to improve the metrics are elucidated.
Pangeo Forge: Crowdsourcing Analysis-Ready, Cloud Optimized Data Production
Authors: Stern Charles , Abernathey Ryan , Hamman Joseph , Wegener Rachel , Lepore Chiara , Harkins Sean , Merose Alexander
Published: February 2022

Pangeo Forge is a new community-driven platform that accelerates science by providing high-level recipe frameworks alongside cloud compute infrastructure for extracting data from provider archives, transforming it into analysis-ready, cloud-optimized (ARCO) data stores, and providing a human- and machine-readable catalog for browsing and loading. In abstracting the scientific domain logic of data recipes from cloud infrastructure concerns, Pangeo Forge aims to open a door for a broader community of scientists to participate in ARCO data production. A wholly open-source platform composed of multiple modular components, Pangeo Forge presents a foundation for the practice of reproducible, cloud-native, big-data ocean, weather, and climate science without relying on proprietary or cloud-vendor-specific tooling.