bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Characterization of Lipoptena fortisetosa from Environmental Samples Collected in North-Eastern Poland
Authors: Gałęcki Remigiusz , Xuan Xuenan , Bakuła Tadeusz , Jaroszewski Jerzy
Published: April 2021

Recent years have witnessed an increase in the population of Lipoptenafortisetosa in Central Europe. The genetic profile of this ectoparasite has not been studied in Poland to date. The aim of the present study was to confirm the presence of L.fortisetosa in north-eastern Poland and to characterize the examined population with the use of molecular methods. Deer keds were collected between June and July 2019 in six natural, mixed forests. A fragment of the rRNA 16S gene was used as a marker to identify L.fortisetosa by polymerase chain reaction (PCR). DNA samples were sequenced in the last step. Six new locations of L. fortisetosa were confirmed. No significant differences were observed in the sex ratios of L. cervi and L. fortisetosa (L. cervi p-value = 0.74; L. fortisetosa p-value = 0.65). Significant differences were noted between the total size of L. cervi and L. fortisetosa populations (p-value < 0.001). The similarity to GenBank sequences ranged from 95.56% to 100%. The obtained nucleotide sequences were very closely related to L. fortisetosa sequences from Lithuania, the Czech Republic, and Japan. Molecular analyses revealed considerable genetic diversity, which could indicate that various ectoparasite lineages have spread throughout Europe.
How Tourist Preference and Satisfaction Can Contribute to Improved Welfare Standards at Elephant Tourism Venues in Thailand
Authors: Flower Emily K. , Burns Georgette Leah , Jones Darryl N.
Published: April 2021

Consumer satisfaction and preference can be integral in influencing and solidifying change in user-driven industries such as tourism. High satisfaction rates are imperative to the continual success of a venue as satisfaction determines the likelihood of repeat business and positive recommendations to friends, family and online review forums. Tourist preference for ecocentric tourism venues, over anthropocentric ones, appears to be increasing in elephant tourism venues (ETVs) in Thailand. To explore this, we visited twelve ETVs in Chiang Mai, Thailand, and compared the preferences and satisfaction of tourists who visited riding and non-riding venues toward the use of captive elephants in an entertainment setting. We found that tourists visited riding and non-riding ETVs for similar reasons, primarily due to recommendations from friends and reviews, and because the venue had a good reputation. Tourist preference for higher welfare standards was observed at venues where participants directly observed poor treatment of the elephants. Tourist satisfaction may be impacted by higher elephant welfare standards; therefore, tourists have the ability to influence the elephant tourism industry by demanding better living conditions for elephants and only financially supporting ETVs with higher welfare standards.
Comparison of Mesh and Barbed Suture for Laparoscopic Nephrosplenic Space Ablation in Horses
Authors: Gialletti Rodolfo , Nannarone Sara , Gandini Marco , Cerullo Anna , Bertoletti Alice , Scilimati Nicola , Giusto Gessica
Published: April 2021

Nephrosplenic space (NSS) ablation has been demonstrated to be an effective technique for prevention of left dorsal displacement of the large colon and multiple laparoscopic techniques, among which ablation with mesh or with a barbed suture, have been proposed. Our objective was to compare two laparoscopic techniques for closure of the NSS in twenty-eight horses diagnosed with nephrosplenic entrapment. Medical records of horses that had laparoscopic NSS ablation in two referral centers between 2017–2019 were retrieved. Duration of surgery, complications, and short- and long-term follow-up information were collected and compared. Costs were also calculated and compared. All horses met the inclusion criteria: 9 had NSS ablation with a mesh implant (group M), 19 with barbed suture material (group B). One horse in group B had recurrent colic after discharge. At control laparoscopy after 5 months, the NSS resulted in still not being ablated because of a failure of the suture. In group M, three horses had recurrent colic. One was successfully treated medically, one died of unknown causes and the third required a second laparoscopic suturing at 3–6 months because of failure of the mesh implant. The mean time of surgery and costs were lower in group B compared to group M. The barbed suture technique was faster, more cost-effective and had a lower complication rate than the mesh implant.
Effect of Marine Algae Supplementation on Somatic Cell Count, Prevalence of Udder Pathogens, and Fatty Acid Profile of Dairy Goats’ Milk
Authors: Pajor Ferenc , Egerszegi István , Szűcs Ágnes , Póti Péter , Bodnár Ákos
Published: April 2021

The aim of this study was to evaluate the effect of the Schizochytrium limacinum marine algae on the milk composition and fatty acid profile, somatic cell count, and prevalence of pathogen bacteria in the raw milk of multiparous Alpine goats. Twenty-eight dairy goats were randomly allocated to two groups: control group (C)—fed with 1500 g alfalfa hay and 600 g concentrate; experimental group (MA)—received the same forages and concentrate supplemented with 10 g/head/day marine algae. The goats were housed indoors, while the experiment lasted five weeks, and the milk samples were taken every week. Marine algae feeding had no negative effect on milk composition. The marine algae inclusion significantly decreased the milk somatic cell count and the presence of udder pathogens in the MA group. Mean somatic cell count and presence of udder pathogens were 5.73 log cells/mL and 31%, respectively, in the C group, while these values were 5.34 log cells/mL and 10%, respectively, in the MA group. The marine algae supplementation significantly increased DHA and rumenic acid concentration in the milk of the MA group (0.32 and 0.99 g/100 g of fatty acids, respectively) compared to the C group (0.04 and 0.65 g/100 g of fatty acids, respectively). It can be concluded that a diet supplemented with marine algae significantly improves the udder health of goats and the concentrations of health-promoting fatty acids in milk.
Microvascularization and Expression of Fibroblast Growth Factor and Vascular Endothelial Growth Factor and Their Receptors in the Mare Oviduct
Authors: Pinto-Bravo Pedro , Rebordão Maria Rosa , Amaral Ana , Fernandes Carina , Galvão António , Silva Elisabete , Pessa-Santos Pedro , Alexandre-Pires Graça , Roberto da Costa Rosário P. , Skarzynski Dariusz J. , Ferreira-Dias Graça
Published: April 2021

The oviduct presents the ideal conditions for fertilization and early embryonic development. In this study, (i) vascularization pattern; (ii) microvascular density; (iii) transcripts of angiogenic factors (FGF1, FGF2, VEGF) and their receptors—FGFR1, FGFR2, KDR, respectively, and (iv) the relative protein abundance of those receptors were assessed in cyclic mares’ oviducts. The oviductal artery, arterioles and their ramifications, viewed by means of vascular injection-corrosion, differed in the infundibulum, ampulla and isthmus. The isthmus, immunostained with CD31, presented the largest vascular area and the highest number of vascular structures in the follicular phase. Transcripts (qPCR) and relative protein abundance (Western blot) of angiogenic factors fibroblast growth factor 1 (FGF1) and 2 (FGF2) and vascular endothelial growth factor (VEGF), and their respective receptors (FGFR1, FGFR2, VEGFR2 = KDR), were present in all oviduct portions throughout the estrous cycle. Upregulation of the transcripts of angiogenic receptors FGF1 and FGFR1 in the ampulla and isthmus and of FGF2 and KDR in the isthmus were noted. Furthermore, in the isthmus, the relative protein abundance of FGFR1 and KDR was the highest. This study shows that the equine oviduct presents differences in microvascular density in its three portions. The angiogenic factors VEGF, FGF1, FGF2 and their respective receptors are expressed in all studied regions of the mare oviduct, in agreement with microvascular patterns.
Determination of Colistin in Contents Derived from Gastrointestinal Tract of Feeding Treated Piglet and Broiler
Authors: Peng Chun , Zuo Sanling , Qiu Yinsheng , Fu Shulin , Peng Lijuan
Published: April 2021

Colistin is considered as the last-resort treatment for multiantibiotic-resistant Gram-negative bacterial infections in humans. However, the oral administration of colistin to livestock and poultry results in the introduction of large amounts of colistin to the surrounding environment via urine and feces, potentially inducing the prevalence of colistin-resistant bacteria and the impact on the ecological environment. We established a quantitative mass spectrometry (MS) based method to measure colistin in contents recovered from the gastrointestinal segments of piglets and broilers, as well as colistin in feces from the animals. The mean recoveries of colistin from different matrices were between 73.2% and 103.9%. The quantitation limit values for different matrices ranged from 0.37 to 1.85 ng/g. In colistin-treated swine samples, the highest concentration of colistin was detected in feces samples at a level of 1248.3 ng/g. However, the highest concentration of colistin in broiler samples was around 4882.9 ng/g, which was found in the contents derived from broilers’ ceca. The employment of the proposed method to assess colistin in animals’ gastrointestinal tracts might help to understand the colistin absorption in animals’ guts and the potential impact of colistin on the emergence of resistant bacteria in animals’ gut flora and the ecological environment.
Mitochondrial Dysfunction Is a Common Denominator Linking Skeletal Muscle Wasting Due to Disease, Aging, and Prolonged Inactivity
Authors: Hyatt Hayden W. , Powers Scott K.
Published: April 2021

Skeletal muscle is the most abundant tissue in the body and is required for numerous vital functions, including breathing and locomotion. Notably, deterioration of skeletal muscle mass is also highly correlated to mortality in patients suffering from chronic diseases (e.g., cancer). Numerous conditions can promote skeletal muscle wasting, including several chronic diseases, cancer chemotherapy, aging, and prolonged inactivity. Although the mechanisms responsible for this loss of muscle mass is multifactorial, mitochondrial dysfunction is predicted to be a major contributor to muscle wasting in various conditions. This systematic review will highlight the biochemical pathways that have been shown to link mitochondrial dysfunction to skeletal muscle wasting. Importantly, we will discuss the experimental evidence that connects mitochondrial dysfunction to muscle wasting in specific diseases (i.e., cancer and sepsis), aging, cancer chemotherapy, and prolonged muscle inactivity (e.g., limb immobilization). Finally, in hopes of stimulating future research, we conclude with a discussion of important future directions for research in the field of muscle wasting.
Analysis of La4Ni3O10±δ-BaCe0.9Y0.1O3-δ Composite Cathodes for Proton Ceramic Fuel Cells
Authors: Loureiro Francisco J. A. , Ramasamy Devaraj , Graça Vanessa C. D. , Holz Laura I. V. , Mikhalev Sergey M. , Fagg Duncan P.
Published: April 2021

Layered Ruddlesden-Popper (RP) lanthanide nickelates, Lnn+1NinO3n+1 (Ln = La, Pr, and Nd; n = 1, 2, and 3) have generated great interest as potential cathodes for proton conducting fuel cells (PCFCs). The high-order phase (n = 3) is especially intriguing, as it possesses the property of a high and metallic-type electronic conductivity that persists to low temperatures. To provide the additional requirement of high ionic conductivity, a composite electrode is here suggested, formed by a combination of La4Ni3O10±δ with the proton conducting phase BaCe0.9Y0.1O3-δ (40 vol%). Electrochemical impedance spectroscopy (EIS) is used to analyse this composite electrode in both wet (pH2O ~ 10−2 atm) and low humidity (pH2O ~ 10−5 atm) conditions in an O2 atmosphere (400–550 °C). An extended analysis that first tests the stability of the impedance data through Kramers-Kronig and Bayesian Hilbert transform relations is outlined, that is subsequently complemented with the distribution function of relaxation times (DFRTs) methodology. In a final step, correction of the impedance data against the short-circuiting contribution from the electrolyte substrate is also performed. This work offers a detailed assessment of the La4Ni3O10±δ-BaCe0.9Y0.1O3-δ composite cathode, while providing a robust analysis methodology for other researchers working on the development of electrodes for PCFCs.
Search Patterns Based on Trajectories Extracted from the Response of Second-Order Systems
Authors: Cuevas Erik , Becerra Héctor , Escobar Héctor , Luque-Chang Alberto , Pérez Marco , Eid Heba F. , Jiménez Mario
Published: April 2021

Recently, several new metaheuristic schemes have been introduced in the literature. Although all these approaches consider very different phenomena as metaphors, the search patterns used to explore the search space are very similar. On the other hand, second-order systems are models that present different temporal behaviors depending on the value of their parameters. Such temporal behaviors can be conceived as search patterns with multiple behaviors and simple configurations. In this paper, a set of new search patterns are introduced to explore the search space efficiently. They emulate the response of a second-order system. The proposed set of search patterns have been integrated as a complete search strategy, called Second-Order Algorithm (SOA), to obtain the global solution of complex optimization problems. To analyze the performance of the proposed scheme, it has been compared in a set of representative optimization problems, including multimodal, unimodal, and hybrid benchmark formulations. Numerical results demonstrate that the proposed SOA method exhibits remarkable performance in terms of accuracy and high convergence rates.
Constructed Technosols: A Strategy toward a Circular Economy
Authors: Fabbri Debora , Pizzol Romeo , Calza Paola , Malandrino Mery , Gaggero Elisa , Padoan Elio , Ajmone-Marsan Franco
Published: April 2021

Soil is a non-renewable natural resource. However, the current rates of soil usage and degradation have led to a loss of soil for agriculture, habitats, biodiversity, and to ecosystems problems. Urban and former industrial areas suffer particularly of these problems, and compensation measures to restore environmental quality include the renaturation of dismissed areas, de-sealing of surfaces, or the building of green infrastructures. In this framework, the development of methodologies for the creation of soils designed to mimic natural soil and suitable for vegetation growth, known as constructed soils or technosols, are here reviewed. The possible design choices and the starting materials have been described, using a circular economy approach, i.e., preferring non-contaminated wastes to non-renewable resources. Technosols appear to be a good solution to the problems of land degradation and urban green if using recycled wastes or by-products, as they can be an alternative to the remediation of contaminated sites and to importing fertile agricultural soil. Nevertheless, waste use requires analysis to ensure the salubrity of the starting materials. Moreover, materials produced on site or nearby minimize the cost and the environmental impact of transport, thus the involvement of local stakeholders in the urban land management must be encouraged.
Biomechanical Analysis of Sagittal Plane Pin Placement Configurations for Pediatric Supracondylar Humerus Fractures
Authors: Pothong Witit , Phinyo Phichayut , Sirirungruangsarn Yuddhasert , Nabudda Kriengkrai , Wongba Nattamon , Sarntipiphat Chatchawarl , Pruksakorn Dumnoensun
Published: April 2021

Anterior to posterior (AP) pinning is the recommended sagittal pin configuration in divergent lateral entry coronal pinning of pediatrics supracondylar fractures. However, there was still a lack of evidence regarding alternative sagittal pins configurations. We aimed to compare the construct stiffness of alternative sagittal pin configurations by using synthetic bone models. Sixty synthetic pediatric humeri were osteotomized to create a supracondylar fracture. After the fracture reduction, all specimens were fixed in the coronal plane with divergent lateral entry pin configurations in four different patterns in the sagittal plane: AP, crossed, divergent and parallel sagittal pin configuration. Each configuration was tested with five loading patterns. The AP sagittal pin had significantly lower construct stiffness than the divergent (p = 0.003) and the parallel sagittal pin configuration (p = 0.005) in external rotation loading tests. The divergent sagittal pin had the highest construct stiffness in extension, valgus, and external rotation loads, but the parallel sagittal pin had lower construct stiffness under extension load than the divergent and crossed sagittal pin configurations. The divergent sagittal pin configuration provides greater construct stiffness than other sagittal pin configurations due to the maximal pin spreading distance at the fracture site and the pin angle lock mechanism.
Addendum: Fang, Q.; Maldague, X. A Method of Defect Depth Estimation for Simulated Infrared Thermography Data with Deep Learning. Appl. Sci. 2020, 10, 6819
Authors: Fang Qiang , Maldague Xavier.
Published: April 2021

The authors wish to make the following corrections to this paper [...]
Directions of Changes in the Content of Selected Macro- and Micronutrients of Kale, Rutabaga, Green and Purple Cauliflower Due to Hydrothermal Treatment
Authors: Kapusta-Duch Joanna , Florkiewicz Adam , Leszczyńska Teresa , Borczak Barbara
Published: April 2021

Little is still known about macro- and micronutrients in processed selected Brassica vegetables such as purple and green cauliflower, rutabaga and kale. This study evaluates the influence of different processing conditions (blanching and boiling) on the stability of selected macro- and micronutrients in the aforementioned vegetables. Results indicated that blanching and boiling affect the mineral content of Brassica vegetables. Of the examined Brassica vegetables, the largest losses were found for potassium and iron (on average by 39.72%).
Susceptibility of Staphylococcus epidermidis to Argon Cold Plasma Jet by Oxygen Admixture
Authors: Mohamed Abdel-Aleam H. , Basher Abdulrahman H. , Almarashi Jamal Q. M. , Ouf Salama A.
Published: April 2021

Cold atmospheric pressure sterilization is one of the nominated and efficient techniques to prevent the spread of diseases. Reactive species such as O and OH and other radicals play a major role in the mechanism of plasma sterilization. Therefore, in this work, oxygen was mixed with different parentage from (0.2 to 1.2%) to argon to enhance the generation of the reactive species and increase the argon atmospheric pressure plasma sterilization efficacy. The emission spectra from the jet increase the radicle line intensities by increasing the percentage admixture of O2 with the argon gas to reach a maximum power at 0.8; then, it gradually decreases with a higher O2 percentage. The OH band intensity decreases with increasing the admixture of O2. The jet with different O2 percentages was tested against Gram-positive S. epidermidis, which is the causal agent of nosocomial infections. The maximum reduction in colony-forming units (CFU) was observed at 0.2% O2. No bacterial growth was observed at the later concentration applied for 8 min and the same case was detected at 0.4% O2 applied to 16 min.
Multiscale Spatiotemporal Analysis of Extreme Events in the Gomati River Basin, India
Authors: Kalyan AVS , Ghose Dillip Kumar , Thalagapu Rahul , Guntu Ravi Kumar , Agarwal Ankit , Kurths Jürgen , Rathinasamy Maheswaran
Published: April 2021

Accelerating climate change is causing considerable changes in extreme events, leading to immense socioeconomic loss of life and property. In this study, we investigate the characteristics of extreme climate events at a regional scale to -understand these events’ propagation in the near future. We have considered sixteen extreme climate indices defined by the World Meteorological Organization’s Expert Team on Climate Change Detection and Indices from a long-term dataset (1951–2018) of 53 locations in Gomati River Basin, North India. We computed the present and future spatial variation of theses indices using the Sen’s slope estimator and Hurst exponent analysis. The periodicities and non-stationary features were estimated using the continuous wavelet transform. Bivariate copulas were fitted to estimate the joint probabilities and return periods for certain combinations of indices. The study results show different variation in the patterns of the extreme climate indices: D95P, R95TOT, RX5D, and RX showed negative trends for all stations over the basin. The number of dry days (DD) showed positive trends over the basin at 36 stations out of those 17 stations are statistically significant. A sustainable decreasing trend is observed for D95P at all stations, indicating a reduction in precipitation in the future. DD exhibits a sustainable decreasing trend at almost all the stations over the basin barring a few exceptions highlight that the basin is turning drier. The wavelet power spectrum for D95P showed significant power distributed across the 2–16-year bands, and the two-year period was dominant in the global power spectrum around 1970–1990. One interesting finding is that a dominant two-year period in D95P has changed to the four years after 1984 and remains in the past two decades. The joint return period’s resulting values are more significant than values resulting from univariate analysis (R95TOT with 44% and RTWD of 1450 mm). The difference in values highlights that ignoring the mutual dependence can lead to an underestimation of extremes.
Color as a New Proxy Technique for the Identification of Road Dust Samples Contaminated with Potentially Toxic Elements: The Case of Mérida, Yucatán, México
Authors: Aguilar Yameli , Bautista Francisco , Quintana Patricia , Aguilar Daniel , Trejo-Tzab Rudy , Goguitchaichvili Avto , Chan-Te Roberto
Published: April 2021

The design of proxy techniques is an innovative tool to monitor the potentially toxic elements of pollution in road dust. This study evaluated the use of road dust color as a proxy methodology to identify samples contaminated with presumably contaminating elements. FRX determined the concentrations of Fe, Ti, Rb, Sr, Y, Cu, Zn, and Pb in eighty-five road dust samples. The appliance of the RGB system and the Munsell color cards identified five color groups of road dust samples. The discriminant analysis validated these groups by colorimetric indices and presumably contaminating elements. The “very dark gray” color of road dust contains the highest concentrations of Pb, Cu, Zn, and Y. The redness and saturation rates showed high correlations with presumably contaminating elements in “dark gray” and “very dark gray” color samples. The color of road dust, as a proxy technique, allows identifying samples contaminated with presumably contaminating elements.
Air Quality and Sustainable Development of Urban Agglomerations in the Mediterranean Area: Science, Technology and Policies
Authors: Murena Fabio , Salizzoni Pietro
Published: April 2021

Air quality in urban areas is one of the main challenges of sustainable development [...]
Towards the Dependence on Parameters for the Solution of the Thermostatted Kinetic Framework
Authors: Carbonaro Bruno , Menale Marco
Published: April 2021

A complex system is a system involving particles whose pairwise interactions cannot be composed in the same way as in classical Mechanics, i.e., the result of interaction of each particle with all the remaining ones cannot be expressed as a sum of its interactions with each of them (we cannot even know the functional dependence of the total interaction on the single interactions). Moreover, in view of the wide range of its applications to biologic, social, and economic problems, the variables describing the state of the system (i.e., the states of all of its particles) are not always (only) the usual mechanical variables (position and velocity), but (also) many additional variables describing e.g., health, wealth, social condition, social rôle ⋯, and so on. Thus, in order to achieve a mathematical description of the problems of everyday’s life of any human society, either at a microscopic or at a macroscpoic scale, a new mathematical theory (or, more precisely, a scheme of mathematical models), called KTAP, has been devised, which provides an equation which is a generalized version of the Boltzmann equation, to describe in terms of probability distributions the evolution of a non-mechanical complex system. In connection with applications, the classical problems about existence, uniqueness, continuous dependence, and stability of its solutions turn out to be particularly relevant. As far as we are aware, however, the problem of continuous dependence and stability of solutions with respect to perturbations of the parameters expressing the interaction rates of particles and the transition probability densities (see Section The Basic Equations has not been tackled yet). Accordingly, the present paper aims to give some initial results concerning these two basic problems. In particular, Theorem 2 reveals to be stable with respect to small perturbations of parameters, and, as far as instability of solutions with respect to perturbations of parameters is concerned, Theorem 3 shows that solutions are unstable with respect to “large” perturbations of interaction rates; these hints are illustrated by numerical simulations that point out how much solutions corresponding to different values of parameters stay away from each other as t→+∞.
Early Detection of Failing Automotive Batteries Using Gas Sensors
Authors: Essl Christiane , Seifert Lauritz , Rabe Michael , Fuchs Anton
Published: April 2021

Safety for automotive lithium-ion battery (LIB) applications is of crucial importance, especially for electric vehicle applications using batteries with high capacity and high energy density. In case of a defect inside or outside the cell, serious safety risks are possible including extensive heat generation, toxic and flammable gas generation, and consequently fire and explosion. New regulations (GB 38031-2020) require a warning for passengers at least five minutes before serious incidents. This regulation can hardly be fulfilled with state-of-the-art battery monitoring. In this study, gases produced during battery failure before and during a thermal runaway (TR) are investigated in detail and the use of different gas sensors as early detectors of battery incidents is tested and proposed. The response of several commercially available gas sensors is tested in four battery failure cases: unwanted electrolysis of voltage carrying parts, electrolyte vapor, first venting of the cell and the TR. The experiments show that battery failure detection with gas sensors is possible but depends highly on the failure case. The chosen gas sensor can detect H2 produced by unwanted electrolysis and electrolyte vapor and gases produced by degassing of state-of-the-art LIBs. The results may contribute significantly to failure detection and improvement of battery safety.
Use of PRP, PRF and CGF in Periodontal Regeneration and Facial Rejuvenation—A Narrative Review
Authors: Mijiritsky Eitan , Assaf Haya Drora , Peleg Oren , Shacham Maayan , Cerroni Loredana , Mangani Luca
Published: April 2021

Growth factors (GFs) play a vital role in cell proliferation, migration, differentiation and angiogenesis. Autologous platelet concentrates (APCs) which contain high levels of GFs make them especially suitable for periodontal regeneration and facial rejuvenation. The main generations of APCs presented are platelet-rich plasma (PRP), platelet-rich fibrin (PRF) and concentrated growth factor (CGF) techniques. The purpose of this review is to provide the clinician with an overview of APCs’ evolution over the past decade in order to give reliable and useful information to be used in clinical work. This review summarizes the most interesting and novel articles published between 1997 and 2020. Electronic and manual searches were conducted in the following databases: Pubmed, Scopus, Cochrane Library and Embase. The following keywords were used: growth factors, VEGF, TGF-b1, PRP, PRF, CGF and periodontal regeneration and/or facial rejuvenation. A total of 73 articles were finally included. The review then addresses the uses of the three different techniques in the two disciplines, as well as the advantages and limitations of each technique. Overall, PRP is mainly used in cases of hard and soft tissue procedures, while PRF is used in gingival recession and the treatment of furcation and intrabony defects; CGF is mainly used in bone regeneration.
Raman Microspectroscopic Analysis of Selenium Bioaccumulation by Green Alga Chlorella vulgaris
Authors: Kizovský Martin , Pilát Zdeněk , Mylenko Mykola , Hrouzek Pavel , Kuta Jan , Skoupý Radim , Krzyžánek Vladislav , Hrubanová Kamila , Adamczyk Olga , Ježek Jan , Bernatová Silvie , Klementová Tereza , Gjevik Alžběta , Šiler Martin , Samek Ota , Zemánek Pavel
Published: April 2021

Selenium (Se) is an element with many commercial applications as well as an essential micronutrient. Dietary Se has antioxidant properties and it is known to play a role in cancer prevention. However, the general population often suffers from Se deficiency. Green algae, such as Chlorella vulgaris, cultivated in Se-enriched environment may be used as a food supplement to provide adequate levels of Se. We used Raman microspectroscopy (RS) for fast, reliable, and non-destructive measurement of Se concentration in living algal cells. We employed inductively coupled plasma-mass spectrometry as a reference method to RS and we found a substantial correlation between the Raman signal intensity at 252 cm−1 and total Se concentration in the studied cells. We used RS to assess the uptake of Se by living and inactivated algae and demonstrated the necessity of active cellular transport for Se accumulation. Additionally, we observed the intracellular Se being transformed into an insoluble elemental form, which we further supported by the energy-dispersive X-ray spectroscopy imaging.
Genetically Encoded Fluorescent Indicators for Imaging Brain Chemistry
Authors: Bi Xiaoke , Beck Connor , Gong Yiyang
Published: April 2021

Genetically encoded fluorescent indicators, combined with optical imaging, enable the detection of physiologically or behaviorally relevant neural activity with high spatiotemporal resolution. Recent developments in protein engineering and screening strategies have improved the dynamic range, kinetics, and spectral properties of genetically encoded fluorescence indicators of brain chemistry. Such indicators have detected neurotransmitter and calcium dynamics with high signal-to-noise ratio at multiple temporal and spatial scales in vitro and in vivo. This review summarizes the current trends in these genetically encoded fluorescent indicators of neurotransmitters and calcium, focusing on their key metrics and in vivo applications.
Detection of Sub-Nanomolar Concentration of Trypsin by Thickness-Shear Mode Acoustic Biosensor and Spectrophotometry
Authors: Piovarci Ivan , Melikishvili Sopio , Tatarko Marek , Hianik Tibor , Thompson Michael
Published: April 2021

The determination of protease activity is very important for disease diagnosis, drug development, and quality and safety assurance for dairy products. Therefore, the development of low-cost and sensitive methods for assessing protease activity is crucial. We report two approaches for monitoring protease activity: in a volume and at surface, via colorimetric and acoustic wave-based biosensors operated in the thickness-shear mode (TSM), respectively. The TSM sensor was based on a β-casein substrate immobilized on a piezoelectric quartz crystal transducer. After an enzymatic reaction with trypsin, it cleaved the surface-bound β-casein, which increased the resonant frequency of the crystal. The limit of detection (LOD) was 0.48 ± 0.08 nM. A label-free colorimetric assay for trypsin detection has also been performed using β-casein and 6-mercaptohexanol (MCH) functionalized gold nanoparticles (AuNPs/MCH-β-casein). Due to the trypsin cleavage of β-casein, the gold nanoparticles lost shelter, and MCH increased the attractive force between the modified AuNPs. Consequently, AuNPs aggregated, and the red shift of the absorption spectra was observed. Spectrophotometric assay enabled an LOD of 0.42 ± 0.03 nM. The Michaelis–Menten constant, KM, for reverse enzyme reaction has also been estimated by both methods. This value for the colorimetric assay (0.56 ± 0.10 nM) is lower in comparison with those for the TSM sensor (0.92 ± 0.44 nM). This is likely due to the better access of the trypsin to the β-casein substrate at the surface of AuNPs in comparison with those at the TSM transducer.
A Carbon-Based Antifouling Nano-Biosensing Interface for Label-Free POCT of HbA1c
Authors: Li Zhenhua , Li Jianyong , Dou Yanzhi , Wang Lihua , Song Shiping
Published: April 2021

Electrochemical biosensing relies on electron transport on electrode surfaces. However, electrode inactivation and biofouling caused by a complex biological sample severely decrease the efficiency of electron transfer and the specificity of biosensing. Here, we designed a three-dimensional antifouling nano-biosensing interface to improve the efficiency of electron transfer by a layer of bovine serum albumin (BSA) and multi-walled carbon nanotubes (MWCNTs) cross-linked with glutaraldehyde (GA). The electrochemical properties of the BSA/MWCNTs/GA layer were investigated using both cyclic voltammetry and electrochemical impedance to demonstrate its high-efficiency antifouling nano-biosensing interface. The BSA/MWCNTs/GA layer kept 92% of the original signal in 1% BSA and 88% of that in unprocessed human serum after a 1-month exposure, respectively. Importantly, we functionalized the BSA/MWCNTs/GA layer with HbA1c antibody (anti-HbA1c) and 3-aminophenylboronic acid (APBA) for sensitive detection of glycated hemoglobin A (HbA1c). The label-free direct electrocatalytic oxidation of HbA1c was investigated by cyclic voltammetry (CV). The linear dynamic range of 2 to 15% of blood glycated hemoglobin A (HbA1c) in non-glycated hemoglobin (HbAo) was determined. The detection limit was 0.4%. This high degree of differentiation would facilitate a label-free POCT detection of HbA1c.
Detection of Influenza Virus Using a SOI-Nanoribbon Chip, Based on an N-Type Field-Effect Transistor
Authors: Malsagova Kristina A. , Pleshakova Tatyana O. , Kozlov Andrey F. , Galiullin Rafael A. , Popov Vladimir P. , Tikhonenko Fedor V. , Glukhov Alexander V. , Ziborov Vadim S. , Shumov Ivan D. , Petrov Oleg F. , Generalov Vladimir M. , Cheremiskina Anastasia A. , Durumanov Alexander G. , Agafonov Alexander P. , Gavrilova Elena V. , Maksyutov Rinat A. , Safatov Alexander S. , Nikitaev Valentin G. , Pronichev Alexander N. , Konev Vladimir A. , Archakov Alexander I. , Ivanov Yuri D.
Published: April 2021

The detection of influenza A virions with a nanoribbon detector (NR detector) has been demonstrated. Chips for the detector have been fabricated based on silicon-on-insulator nanoribbon structures (SOI nanoribbon chip), using a complementary metal-oxide-semiconductor (CMOS)-compatible technology—by means of gas-phase etching and standard optical photolithography. The surface of the SOI nanoribbon chip contains a matrix of 10 nanoribbon (NR) sensor elements. SOI nanoribbon chips of n-type conductance have been used for this study. For biospecific detection of target particles, antibodies against influenza virus have been covalently immobilized onto NRs. Influenza A virus detection was performed by real-time registration of the source-drain current through the NRs. The detection of the target viral particles was carried out in buffer solutions at the target particles concentration within the range from 107 to 103 viral particles per milliliter (VP/mL). The lowest detectable concentration of the target viral particles was 6 × 10−16 M (corresponding to 104 VP/mL). The use of solutions containing ~109 to 1010 VP/mL resulted in saturation of the sensor surface with the target virions. In the saturation mode, detection was impossible.
Migraine during COVID-19: Data from Second Wave Pandemic in an Italian Cohort
Authors: Gentile Eleonora , Delussi Marianna , Abagnale Chiara , Caponnetto Valeria , De Cesaris Francesco , Frattale Ilaria , Guaschino Elena , Marcinnò Andrea , Ornello Raffaele , Pistoia Francesca , Putortì Alessia , Candida Giusy , Roveta Fausto , Lupi Chiara , Coppola Gianluca , Prudenzano Addolorata Maria Pia , Rainero Innocenzo , Sances Grazia , Roca Maria Elena , Trojano Maria , Pierelli Francesco , Geppetti Pierangelo , Sacco Simona , de Tommaso Marina
Published: April 2021

Objectives: The study aims to assess the impact of the second COVID-19 pandemic wave on migraine characteristics. Methods: This is an observational cross-sectional study conducted on migraine patients previously interviewed during the first Italian pandemic outbreak. A second structured telephone interview was conducted between 20 November 2020 and 18 January 2021. We compared migraine characteristics among T0 (before pandemic), T1 (during the first pandemic phase), and T2 (during the second pandemic phase). Results: Among the 433 patients interviewed during the first pandemic phase, 304 cases were finally considered. One hundred forty-eight patients had a control visit between March 2020 and December 2020, 120 had an in-person visit, 14 by phone, the remainder used telemedicine software provided by the hospital. Frequency of headache, number of symptomatic drugs and headache intensity worsened during T2, compared to T0 and T1, especially in episodic migraine. Headache intensity increased relating to the negative emotional impact of the pandemic. Migraine management during the pandemic did not influence the clinical outcome. Conclusion: The prolongation of the pandemic seems to have a negative impact on migraine evolution. The arousal and negative psychological behavior toward the COVID-19 outbreak seem to worsen migraine.
Null Effect of Transcranial Static Magnetic Field Stimulation over the Dorsolateral Prefrontal Cortex on Behavioral Performance in a Go/NoGo Task
Authors: Watanabe Tatsunori , Kubo Nami , Chen Xiaoxiao , Yunoki Keisuke , Matsumoto Takuya , Kuwabara Takayuki , Sunagawa Toru , Date Shota , Mima Tatsuya , Kirimoto Hikari
Published: April 2021

The purpose of this pilot study was to investigate whether transcranial static magnetic field stimulation (tSMS), which can modulate cortical excitability, would influence inhibitory control function when applied over the dorsolateral prefrontal cortex (DLPFC). Young healthy adults (n = 8, mean age ± SD = 24.4 ± 4.1, six females) received the following stimulations for 30 min on different days: (1) tSMS over the left DLPFC, (2) tSMS over the right DLPFC, and (3) sham stimulation over either the left or right DLPFC. The participants performed a Go/NoGo task before, immediately after, and 10 min after the stimulation. They were instructed to extend the right wrist in response to target stimuli. We recorded the electromyogram from the right wrist extensor muscles and analyzed erroneous responses (false alarm and missed target detection) and reaction times. As a result, 50% of the participants made erroneous responses, and there were five erroneous responses in total (0.003%). A series of statistical analyses revealed that tSMS did not affect the reaction time. These preliminary findings suggest the possibility that tSMS over the DLPFC is incapable of modulating inhibitory control and/or that the cognitive load imposed in this study was insufficient to detect the effect.
Suicidal Behavior and Club Drugs in Young Adults
Authors: Martinotti Giovanni , Schiavone Stefania , Negri Attilio , Vannini Chiara , Trabace Luigia , De Berardis Domenico , Pettorruso Mauro , Sensi Stefano L. , Di Giannantonio Massimo
Published: April 2021

Psychoactive drugs play a significant role in suicidality when used for intentional overdose or, more frequently, when the intoxication leads to disinhibition and alterations in judgment, thereby making suicide more likely. In this study, we investigated suicidality prevalence among drug users and evaluated the differences in suicide ideation, taking into account the substance categories and the association of suicide ideation intensity with other psychiatric symptoms. Subjects admitted to the Can Misses Hospital’s psychiatry ward in Ibiza were recruited during summer openings of local nightclubs for four consecutive years starting in 2015. The main inclusion criterium was an intake of psychoactive substances during the previous 24 h. The Columbia Suicide Severity Rating Scale (C-SSRS) was used to assess the suicide risk. Suicidality was present in 39% of the study cohort. Suicide Ideation Intensity overall and in the previous month was higher in users of opioids and in general of psychodepressors compared to psychostimulants or psychodysleptics. Suicidality was not correlated with alterations in any of the major psychopathological scales employed to assess the psychiatric background of the study subjects. The presence of high levels of suicidality did not specifically correlate with any major symptom indicative of previous or ongoing psychopathological alterations. These findings suggest that impulsivity and loss of self-control may be determinants of the increased suicidality irrespectively of any major ongoing psychiatric background.
Natural Trienoic Acids as Anticancer Agents: First Stereoselective Synthesis, Cell Cycle Analysis, Induction of Apoptosis, Cell Signaling and Mitochondrial Targeting Studies
Authors: D’yakonov Vladimir A. , Makarov Alexey A. , Dzhemileva Lilya U. , Ramazanov Ilfir R. , Makarova Elina Kh. , Dzhemilev Usein M.
Published: April 2021

The first Z-stereoselective method was developed for the synthesis of unsaturated acids containing a 1Z,5Z,9Z-triene moiety in 61–64% yields using the new Ti-catalyzed cross-coupling of oxygen-containing and aliphatic 1,2-dienes as the key synthetic step. It was shown for the first time that trienoic acids with non-methylene-interrupted Z-double bonds show moderate cytotoxic activities against tumor cell lines (Jurkat, K562, U937, HL60, HeLa), human embryonic kidney cells (Hek293), normal fibroblasts and human topoisomerase I (hTop1) inhibitory activity in vitro. The synthesized acids efficiently initiate apoptosis of Jurkat tumor cells, with the cell death mechanism being activated by the mitochondrial pathway. A probable mechanism of topoisomerase I inhibition was also hypothesized on the basis of in silico studies resorting to docking. The activation and inhibition of the most versatile intracellular signaling pathways (CREB, JNK, NFkB, p38, ERK1/2, Akt, p70S6K, STAT3 and STAT5 tyrosine kinases) responsible for cell proliferation and for initiation of apoptosis were studied by multiplex assay technology (Luminex xMAP).
A Systematic Review of the Use of Circulating Cell-Free DNA Dynamics to Monitor Response to Treatment in Metastatic Breast Cancer Patients
Authors: Jongbloed Elisabeth M. , Deger Teoman , Sleijfer Stefan , Martens John W. M. , Jager Agnes , Wilting Saskia M.
Published: April 2021

Monitoring treatment response in metastatic breast cancer currently consists mainly of radiological and clinical assessments. These methods have high inter-observer variation, suboptimal sensitivity to determine response to treatment and give little insight into the biological characteristics of the tumor. Assessing circulating tumor DNA (ctDNA) over time could be employed to address these limitations. Several ways to quantify and characterize ctDNA exist, based on somatic mutations, copy number variations, methylation, and global circulating cell-free DNA (cfDNA) fragment sizes and concentrations. These methods are being explored and technically validated, but to date none of these methods are applied clinically. We systematically reviewed the literature on the use of quantitative ctDNA measurements over time to monitor response to systemic therapy in patients with metastatic breast cancer. Cochrane, Embase, PubMed and Google Scholar databases were searched to find studies focusing on the use of cfDNA to longitudinally monitor treatment response in advanced breast cancer patients until October 2020. This resulted in a total of 33 studies which met the inclusion criteria. These studies were heterogeneous in (pre-)processing procedures, applied techniques and design. An association between ctDNA and treatment response was found in most of the included studies, independent of the applied assay. To implement ctDNA-based response monitoring into daily clinical practice for metastatic breast cancer patients, sample (pre-) processing procedures need to be standardized and large prospectively collected sample cohorts with well annotated clinical follow-up are required to establish its clinical validity.
Radical Resection for Locally Advanced Pancreatic Cancers in the Era of New Neoadjuvant Therapy—Arterial Resection, Arterial Divestment and Total Pancreatectomy
Authors: Inoue Yosuke , Oba Atushi , Ono Yoshihiro , Sato Takafumi , Ito Hiromichi , Takahashi Yu
Published: April 2021

Aggressive arterial resection (AR) or total pancreatectomy (TP) in surgical treatment for locally advanced pancreatic cancer (LAPC) had long been discouraged because of their high mortality rate and unsatisfactory long-term outcomes. Recently, new chemotherapy regimens such as FOLFIRINOX or Gemcitabine and nab-paclitaxel have provided more adequate patient selection and local tumor suppression, justifying aggressive local resection. In this review, we investigate the recent reports focusing on arterial resection and total pancreatectomy for LAPC and discuss the rationale of such an aggressive approach in the treatment of PC. AR for LAPCs is divided into three, according to the target vessel. The hepatic artery resection is the simplest one, and the reconstruction methods comprise end-to-end, graft or transposition, and no reconstruction. Celiac axis resection is mainly done with distal pancreatectomy, which allows collateral arterial supply to the liver via the pancreas head. Resection of the superior mesenteric artery is increasingly reported, though its rationale is still controversial. Total pancreatectomy has been re-evaluated as an effective option to balance both the local control and postoperative safety. In conclusion, more and more aggressive pancreatectomy has become justified by the principle of total neoadjuvant therapy. Further technical standardization and optimal neoadjuvant strategy are mandatory for the global dissemination of aggressive pancreatectomies.
Find the Flame: Predictive Biomarkers for Immunotherapy in Melanoma
Authors: Garutti Mattia , Bonin Serena , Buriolla Silvia , Bertoli Elisa , Pizzichetta Maria Antonietta , Zalaudek Iris , Puglisi Fabio
Published: April 2021

Immunotherapy has revolutionized the therapeutic landscape of melanoma. In particular, checkpoint inhibition has shown to increase long-term outcome, and, in some cases, it can be virtually curative. However, the absence of clinically validated predictive biomarkers is one of the major causes of unpredictable efficacy of immunotherapy. Indeed, the availability of predictive biomarkers could allow a better stratification of patients, suggesting which type of drugs should be used in a certain clinical context and guiding clinicians in escalating or de-escalating therapy. However, the difficulty in obtaining clinically useful predictive biomarkers reflects the deep complexity of tumor biology. Biomarkers can be classified as tumor-intrinsic biomarkers, microenvironment biomarkers, and systemic biomarkers. Herein we review the available literature to classify and describe predictive biomarkers for checkpoint inhibition in melanoma with the aim of helping clinicians in the decision-making process. We also performed a meta-analysis on the predictive value of PDL-1.