bluuebunny/crossref_metadata_2025 huggingface dataset:
BRD9 Expression, Alteration, Survival And Pathway Analysis In 11 Independent Prostate Cancer Cohorts
Authors: Barma Nafisa , Stone Timothy C , Carmona Echeverria Lina Maria , Heavey Susan
Published: September 2021

Background and aims: Despite recent advances in advanced prostate cancer treatments, there are no clinically useful biomarkers or treatments for men with such cancers. Targeted therapies have shown promise, but there remain fewer actionable targets in prostate cancer than in other cancers. This work aims to characterize BRD9, currently understudied in prostate cancer, and investigate its co-expression with other genes to assess its potential as a biomarker and therapeutic target in human prostate cancer. Materials and methods: Omics data from a total of 2053 prostate cancer patients across 11 independent datasets were accessed via Cancertool and cBioPortal. mRNA expression and co-expression, mutations, amplifications, and deletions were assessed with respect to key clinical parameters including survival, Gleason grade, stage, progression and treatment. Network and pathway analysis was carried out using Genemania, and heatmaps were constructed using Morpheus. Results: BRD9 is overexpressed in prostate cancer patients, especially those with metastatic disease. BRD9 expression did not differ in patients treated with second generation antiandrogens versus those who were not. BRD9 is co-expressed with many genes in the SWI/SNF and BET complexes, as well as those in common signaling pathways in prostate cancer. Summary and conclusions: BRD9 has potential as a diagnostic and prognostic biomarker in prostate cancer. BRD9 also shows promise as a therapeutic target, particularly in advanced prostate cancer, and as a co-target alongside other genes in the SWI/SNF and BET complexes, and those in common prostate cancer signalling pathways. These promising results highlight the need for wider experimental inhibition and co-targeted inhibition of BRD9 in vitro and in vivo, to build on the limited inhibition data available.
Eating Disorder Symptoms and Body Image in Adolescents from Public Schools in Brazil
Authors: Dias Roges Ghidini , Rech Ricardo Rodrigo , Halpern Ricardo
Published: September 2021

There is growing recognition of the adverse effects of body image dissatisfaction (BID) and eating disorder (ED) symptoms on adolescent health. The aim of this study was to estimate the prevalence of ED symptoms, BID, and their relationship in adolescents from public schools in Southern Brazil. A total of 782 schoolchildren (male: n=420, female: n=362); age: 15 ± 0,4 years) answered a self-administrated questionnaire to identify sociodemographic data. Children´s Figure Rating Scale was adopted to identify body image and Eating Attitudes Test (EAT-26) was applied to investigate ED symptoms. Inferential statistics and hierarchical model-controlled logistic regression were used for association between variables. Most of the schoolchildren reported being satisfied with their bodies. However, we observed a higher prevalence of dissatisfaction among girls for being overweight and thinness among boys. Female students and students from schools located in the central area of the city showed higher chances of developing ED symptoms, and the absence of symptoms of ED appeared to act as a protective factor against BID in schoolchildren. Results of this study show the need to reflect on these factors that influence the development of ED and non-acceptance of their own body in a population concerned with their physical appearance.
Devletlerarası İlişkilerde Kimlik: Mursi Dönemi Türkiye-Mısır İlişkileri
Authors: ÇELİKCAN Harun , CAVLAK Hakan
Published: September 2021

Türkiye ve Mısır ortak bir tarihsel geçmişe sahip olmalarına rağmen, ilişkileri kuruluşlarından itibaren rekabet ve krizlerin gölgesinde seyretmiştir. Uzun yıllar mesafeli sürdürülen ilişkiler, Arap Baharı sürecinde Mısır’da Muhammed Mursi ile Müslüman Kardeşlerin iktidar olması ile hızla değişmeye başlamış ve ikili ilişkilerin altın çağı olarak kabul edilebilecek bir döneme girilmiştir.

Amaç: Makalenin amacı, Muhammed Mursi İktidarı döneminde Mısır ile Türkiye arasında değişen ilişkileri iki devletin o dönem sahip olduğu kimlikleri üzerinden açıklayarak kimliğin devletlerarası ilişkileri inşa edici rolünü ortaya koymaktır.

Tasarım/Yöntem: Makale tasarımı, sosyal inşacı yaklaşımın varsayımları ışığında ve devlet kimliklerinin dinamik ve değişken bir yapıda olduğu göz önüne alınarak bu dönemde iki devlet kimliğinin ne şekilde değiştiği ve değişen kimlikle beraber ilişkilerin nasıl yeni bir boyut kazandığı soruları üzerine inşa edilmiştir.

Sınırlılıklar: Türkiye-Mısır ilişkilerini incelerken makalenin odağı kimlik olmuştur, ilişkileri etkileyen diğer unsurlara araştırmanın kapsamını sınırlamak ve tasarımına sadık kalmak için bilinçli olarak değinilmemiştir.

Bulgular: Makalede, iki hükümetin birbirlerine yönelik algılarını ve politikalarını anlamak için iki tarafın kimlikleri ve kimliklerinin oluşum süreci incelenmiştir. Söz konusu dönemde Mısır hükümetini oluşturan Müslüman Kardeşler ile Türkiye hükümetini oluşturan Adalet ve Kalkınma Partisi’nin başkanının ve kurucularının önemli bir kısmının etkilendiği Milli Görüş hareketinin fikirsel düzlemde birbirlerine benzedikleri görülmüştür.

Özgünlük/Değer: Türkiye-Mısır ilişkilerinin, Adalet ve Kalkınma Partisi ve Mursi döneminde iyileşmesinde ve siyasi kimliklerinin oluşumunda her iki hükümetin üyelerinin benzer bir ideolojik geçmişten gelmelerinin önemli bir faktör olduğu tespit edilmiştir. Sahip oldukları ortak kimliğin hükümetleri aracılığı ile devletleri etkilediği ve bunun sayesinde de kolaylıkla ortak bir dil geliştirebildikleri ve çıkarlarını ortaklaştırabildikleri iddia edilmiştir. Diğer bir deyişle, ortak veya benzer kimliğe sahip hükümetlerin, devletlerarası ilişkileri geliştirdiği öne sürülmüştür.
Gunshot Fracture of the Femoral Neck: Internal Fixation and Immediate Proximal Femoral Valgus Osteotomy
Authors: Georgiadis Gregory M. , Andrews Kyle A. , Redfern Roberta E.
Published: September 2021

Case:

We report on a 22-year-old patient with a comminuted femoral neck fracture because of a low-velocity gunshot wound. Treatment consisted of reduction and internal fixation with a fixed angled blade plate and immediate valgus osteotomy. He went on to uneventful healing and an excellent clinical result.

Conclusions:

A comminuted gunshot femoral neck fracture in a young patient is a rare and potentially devastating injury. If stable, and there are no other associated emergent injuries, reduction, internal fixation, and immediate valgus osteotomy should be considered.
Grain size and transport biases in an Ediacaran detrital zircon record
Authors: Cantine Marjorie D. , Setera Jacob B. , Vantongeren Jill A. , Mwinde Chiza , Bergmann Kristin D.
Published: September 2021

Detrital-zircon records of provenance are used to reconstruct paleogeography, sediment sources, and tectonic configuration. Recognition of biases in detrital-zircon records that result from grain-size-dependent processes adds new complexity and caution to the interpretation of these records. We begin by investigating possible size-dependent biases that may affect interpretation of detrital-zircon provenance records in an idealized sedimentary system. Our modeling results show that settling and selective entrainment can differentially affect detrital-zircon spectra if an initial size variation between source zircon populations exists. We then consider a case study: a detrital-zircon record from Ediacaran to Terreneuvian strata of Death Valley, USA, with a focus on the Rainstorm Member of the Johnnie Formation. The detrital-zircon record of the Rainstorm Member shows that despite its unusual, heavy-mineral-rich character, the provenance of the unit is like other units in the succession. Size and density measurements of the grains of the deposit suggest that its enriched heavy-mineral suite is best explained through concentration by selective entrainment and winnowing. The relationship between detrital-zircon grain size and age for samples from the Johnnie Formation are consistent with grain-size influence on the interpretation of provenance, especially for large Grenville-age (1.0–1.2 Ga) zircons. Grain size can exert significant bias on a provenance interpretation and must be accounted for in provenance studies.
Micro-credentials: A learner value framework
Authors: Oliver Beverley
Published: September 2021

Much has been made of micro-credentials, and the ‘craze’ (Ralston, 2021) and ‘hype’ (Roy & Clark, 2019) they generate. One of the barriers to their success is that a definition of micro-credentials has not been widely agreed (Kato, Galán-Muros, & Weko, 2020). However, to succeed, micro-credentials need not just a definition, but a way to ensure they are valued, and bring value to key stakeholders: particularly learners, employers and providers. To this end, this provocation proposes a micro-credential value framework that sets out their key benefits and costs for learners.
Introduction to the 'Beyond Japanese Studies' Special Issue
Authors:   University of New England, McClelland Gwyn
Published: September 2021

Introduction to New Voices in Japanese Studies, Volume 13 [Special Issue] Beyond Japanese Studies: Challenges, Opportunities and COVID-19
Investigation of the Study and Application of Sepsis Guidelines by Physicians in Tertiary Hospitals in China
Authors: Han Silu , Cui Lijian , Qu Yifan , Tian Tian , Wei Bing , Wang Junyu , Yang Jun
Published: September 2021

Background: To investigate the current status and problems of sepsis guidelines in clinical application among doctors in tertiary hospitals in China and to provide evidence-based case for the formulation, implementation, and training on sepsis diagnosis and treatment.Methods: Physicians from tertiary hospitals, mainly in emergency and critical care departments were enrolled into the survey. Participants completed a questionnaire on sepsis score, diagnostic indicators, fluid resuscitation, antibiotics choice, access to knowledge and application training, and the application of sepsis guidelines in clinical diagnosis and treatment. Results: Of 661 questionnaires that were distributed, 625 were returned. A total of 432 (69.12%) respondents had junior and intermediate professional titles and 193 (30.88%) had senior titles. Among them, 291 (46.56%) were self-taught, 148 (23.68%) were attending lectures, 153 (24.48%) were assigned ward rounds by senior doctors, 9 (30.88%) were taught in other ways, and 24 (3.84%) had no training. To learn about sepsis guidelines, 36.64% of respondents preferred attending lectures, 34.72% chose to study by themselves, and 27.04% preferred listening to senior doctors. Regarding the best way to learn about sepsis guidelines, 42.08% preferred to read by themselves, 41.76% preferred attending lectures, and 14.72% preferred listening to senior doctors. For formulation and implementation of sepsis diagnosis and treatment strategy, >90% of the respondents thought that they needed to rapidly give patients large volumes of fluid replacement but were not satisfied, while 89.6% doctors agreed that rehydration should follow the principle of “use with vasoactive drugs, not too much”. Of the respondents, 61.92% preferred empirical drugs in the use of sepsis antibiotics, 34.56% preferred high-level antibiotics with a wide coverage strategy, 2.4% would choose following instructions by senior doctors, and 0.16% did not know how to choose. Over 70% of the doctors surveyed thought that self-study guide, attending lectures, and senior doctors’ ward rounds were the most important factors affecting drug use, while 47.68% said that the information found on the internet was an important way of guiding drug use.Conclusion: Although the prevalence of sepsis guidelines is high among doctors in tertiary Chinese hospitals, homogeneity of clinical application is poor and greatly affects diagnosis and treatment. Thus, standardized training for sepsis diagnosis and treatment is needed.
Analysis on Impact of Acid Hydrolysis on the Hydrogen Bonding Network in Cellulose to Obtain Microcrystalline Cellulose: A Statistical Approach
Authors: Muthusamy Vishnu Prabha , Krishnakumar Vaideki
Published: September 2021

Hydrolysis of a cellulose biomass results in breaking down the cellulose microfibrils into microcrystalline cellulose (MCC) or nanocrystalline cellulose (NCC) depending on the reaction conditions. Cellulose microfibrils are established robustly due to the synergistic interaction of van der Waals, inter- and intra-molecular hydrogen bonds and glycosidic bond between glucan moieties of cellulose polysaccharide. The hydrogen bonding network plays a crucial role in conforming cellulose chains into crystalline and amorphous region thereby determining its degree of crystallinity. The knowledge of hydrogen bonds in cellulose hence becomes indispensable to understand the crystallinity of cellulose before and after a hydrolysis reaction. However, the nature of hydrogen bonds after hydrolysis and how they contribute to the mechanical properties of resultant MCC/NCC are yet to be realized. This paper is therefore intended to discuss the degree of crystallinity of cellulose particles obtained after hydrolyzing waste cotton fibers (WCF) in two parts: part I, obtaining MCC with maximum total crystallinity index (TCI) by acid hydrolysis of WCF using Box Behnken Design; part II, comparing degree of crystallinity of MCC sample exhibiting highest TCI with that of WCF using analytical tools like X-ray Photoelectron Spectrometer, X-ray Diffractometer and Fourier Transform Infra- Red spectrometer. The physical dimension of MCC particle with maximum TCI has been verified using Field Emission Scanning Electron Microscopic images.
A Five-Immune-Related lncRNA Signature Predicts Survival of Patients With Osteosarcoma
Authors: Huang Jie , Lai Hongyi , Qin Wentao , Bo Zhandong , Tan Zhen , Wu Zhenjie
Published: September 2021

Background: Osteosarcoma (OS) is the most common primary solid malignant bone tumor, and its metastasis is a prominent cause of high mortality in patients.Methods: A risk signature was constructed based on re-annotating the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) data matrix, of the lncRNAs related to OS prognosis and immunity. From the OS transcription data, which is downloaded from the TARGET, the 1126 lncRNAs those harbour co-expressions with immunity genes were selected by Pearson correlation test and later divided into the training set (n=44) and validation set (n=41) with the caret package of R. With the training set we build the model related to Osteosarcoma prognosis by the univariate and multivariate Cox, and the Lasso regression analysis, and in combination with the clinical factors we conducted the multivariate Cox regression analysis to build the 1-year, 3-year and 5-year survival rate nomograms. Afterwards, we validated the ROC and the calibration curve of the subjects with the validation set and the whole dataset. Lastly, we performed functional enrichment analysis with the GSEA, GO and KEGG to figure out the biological functions of the prognosis genes.Results: The training set was performed in univariate and multivariate Cox regression analysis, identifying 25 lncRNAs correlated with prognosis. Eleven lncRNAs were selected by the least absolute shrinkage and selection operator (LASSO) regression for multivariate cox analysis and Kaplan-Meier (KM) survival analysis. Finally, lncRNAs (RP11-69E11.4, SNHG6, MIR210HG, RP11-750H9.5 and CTD-2341M24.1) risk signature was constructed, and the validation set and the whole dataset were used to evaluate the prediction stability and accuracy of the signature. The survival times of high- and low-risk groups were significantly different in the training set, validation set and the whole dataset. Further, function enrichment and gene set enrichment analysis revealed that the lncRNAs in the signature may affect the proliferation, migration, chemotaxis and combination of Osteosarcoma-related immune cells, and involve in every pathways of OS metabolism. Conclusion: The five lncRNAs survival risk signature could potentially predict the prognosis of OS patients, additionally, may provide novel insights for future clinical diagnosis and treatment of OS.
Accumulation and Potential Ecological Risk of Heavy Metals in the Sediments of Rivers System in Beijing-Tianjin Area
Authors: Yuan Shengguang , zhang wenqiang , Li Wenye , Li Zhenhan , Wu Minshan , Shan Baoqing
Published: September 2021

Human activities cause heavy metals to enter the water body and consequently deposit in sediment with slow flow velocity, however little studies have explored the spatial distribution and ecological risk of heavy metals in sediments. The risk, spatial distribution and toxicity of heavy metals in sediment were investigated along the North Canal in the Beijing-Tianjin area. The study revealed that the mean concentrations of heavy metals in sediments exhibited a descending order of Zn > Cr > As > Cu > Pb > Ni > Co > Cd. The average geoaccumulation index (Igeo) value of Cd was highest and ranged from 0.2 to 2.91. Moreover, the greatest contamination of Cd (the Igeo values > 2) was observed in three sampling sites around the Tianjin City. The pollution load index (PLI) of all sampling points were greater than 1, which indicated the inflow of heavy metals originated from anthropogenic sources. The risk index (RI) values of three sampling points were greater than 300, which demonstreated high potential ecological risk. With regards to the toxicity assessment of combined heavy metals, there were two probable effect concentration (PEC) quotient (Qm-PEC) values greater than 0.5 which suggested potential toxicity to certain sediment-dwelling organisms. Identification of the possible sources and factors contributing to the content and spatial distribution of heavy metals could assist in improvement of the water quality, as well as support efficient management strategies to restoration of the environment.
Hyperbaric Oxygen Therapy for the Adjunctive Treatment of Stroke Associated with Post-Cardiac Surgery Due To Native-Valve Endocarditis Infected by Cutibacterium Acnes: A Case Report
Authors: Chen Pei-Ku , Kuo Tzu-Ting , Lin Szu-Ying , Kao Jiang-Hwa , Chen Yen-Wen
Published: September 2021

Background
Stroke is an uncommon but significant complication of cardiac surgery. Cutibacterium (previously known as Propionibacterium) rarely causes infective endocarditis (IE), accounting for approximately 0.3% of all IE cases. Hyperbaric oxygen therapy (HBOT), involving the administration of 100% oxygen at a pressure of > 1.4 atmospheres absolute, increases the partial pressure of oxygen; therefore, it is recommended as an adjunctive treatment for stroke and some infections. However, there are no data supporting HBOT as an adjunctive therapeutic option for ischemic stroke related to post-cardiac surgery due to IE caused by Cutibacterium acnes.
Case presentation:
This study reports the case of an 68-year-old male patient who underwent cardiac surgery for native-valve IE caused by C. acnes. He underwent HBOT on postoperative day 11 for the treatment of ischemic stroke. The patient received 20 sessions of HBOT along with 6-week antimicrobial treatment with ceftriaxone and rehabilitation programs.
Conclusions
We have summarized the patient’s successful recovery and our clinical experience regarding the use of HBOT in our clinical setting. HBOT is an effective adjunctive therapeutic option for ischemic stroke related to post-cardiac surgery due to IE caused by C. acnes.
A Clinical Trial of Cognitive Behavior Therapy for Psychiatric Comorbidity and Quality of Life with Cancer Patients during Chemotherapy (CPdC)
Authors: Abbas Qasir , Arooj Nimra , Baig Khawer Bilal , Khan Muhammad Umer , Khalid Muhammad , Shahzadi Mafia
Published: September 2021

BackgroundCancer is a worldwide common illness, it evokes psychological distress at different stages, during chemotherapy patient perceives a variety of psychiatric symptoms due to various medication side-effects and psychological distress. Studies have shown a significant impact of CBT in the management of psychiatric symptoms during chemotherapy. This study aims to investigate the effectiveness of CBT for depression, anxiety, stress, death anxiety, satisfaction with life, and self-esteem among cancer patients during chemotherapy (CPdC).MethodsPlace and duration of the study: Department of Applied Psychology, Government College University Faisalabad in collaboration with Department of Oncology, Allied Hospital Faisalabad from November 20, 2020 and July 31, 2021. A total of 90 cancer patients were enrolled. 70 out of 90 met the eligibility criteria and 60 participants fulfilled all requirements. Participants were randomly allocated to four different groups. The pre-assessment screening was started along with the first trial of chemotherapy. The CBT-based treatment plan was formulated and one session per week was given to each patient for 3 to 4months. Participants’ age range was 18–65 years (M ± SD = 47.51 ± 12.36. Demographic form, DASS, DAS, SWLS, and RSES measures were used. Descriptive, t-test, and repeated measure ANOVA statistics were used to investigate the findings.ResultsResults indicated significant mean difference on the variable of depression, anxiety and stress across four conditions (i.e. F (2, 56) = 39.55, p < .000, η2 = .679; F(2,56) = 73.32, p < .000, η2 = .797; F(2,56) = 119.77, p < .000, η2 = .865 respectively). On death anxiety significant difference across four conditions was found (F (2,56) = 22.71, p < .000, η2 = .549) with large effect size. Furthermore, findings indicated significant mean difference on the variable of satisfaction with life and self-esteem across four conditions was found (F(2,56) = 22.05, p < .000, η2 = .542; F(2,56) = 36.19, p < .000, η2 = .660) with large effect size.ConclusionIt is concluded that CBT played a very effective role to reduce depression, anxiety, and stress-related psychiatric symptoms. CBT reduces the level of death anxiety and improving the quality of life and level of self-esteem among CPdC.Trial Registration: The study trial was registered in the Thai Clinical Trial Registry-TCTR (TCTR20201113002).
A deformed model for N-type four-level atom and a single mode field system in the presence of the Kerr medium
Authors: Abdel-Wahab N.H. , Abdel-Khalek S. , Khalil E.M. , Alshehri Nawal
Published: September 2021

The aim of this paper is to study the interaction between a single mode field and four-level atom in N - configuration under nonlinear medium effect. The non-resonance case and the deformation forms in the coupling interaction between the field and the atom are included. The wave function of the proposed system is obtained when the atom is prepared initially in its excited state while the field is prepared in a coherent state. The effect of the deformation and nonlinear medium on the temporal behavior of collapse-revival, field entropy and geometric phase of the system are examined. The results show that the presence of the intensity of the coupling interaction and the non-linear medium have an important influence on the properties of these phenomena.
Ankle-Brachial Index: A Simple and Inexpensive Screening Test for Coronary Artery Disease (ABI Goes Beyond the Foot) Running Title: ABI for Coronary Artery Disease Screening
Authors: Vafa Reza Golchin , Zamiri Bardia , Rahmani Mohammadhossein , Parizi Mohammad javad Mehdizadeh , Heydarzadeh Reza , Ahmadi Amin , Montaseri Mohammad , Kojuri Javad
Published: September 2021

Background: Coronary artery disease (CAD) is the leading cause of death in the world. To prevent CAD we need to assess the risk of future CAD. Systems for risk assessment has its own limitations. In this study we assessed ankle-brachial index (ABI) as a screening tool for CAD.Method: Between 2019 and 2020, we conducted a large cross-sectional population-based study of 4207 new patients referred to cardiovascular Clinic. The patients underwent selective coronary angiography via radial artery approach by an expert interventional cardiologist. ABI was calculated for all patients. We compared ABI with the results of coronary angiography to determine the specificity and sensitivity of ABI as a screening tool for CAD. Results: Abnormal ABI was significantly more frequent in patients with proven severe CAD (893, 54.8%) than in patients with proven mild CAD (33, 4.7%) or no CAD (94, 5.3%). The specificity of ABI was 95.3%, and its sensitivity was 54.8%. ABI was associated with risk factors such as smoking, male gender, hypertension, diabetes mellitus and dyslipidemia. There was no relationship between abnormal inter-arm systolic pressure difference and severity of CAD, but it showed a significant association with abnormal ABI.Conclusion: ABI can be used as a screening test to rule out CAD with 95.3% specificity. We need to consider risk factors other than ABI to increase screening sensitivity. A multidimensional scoring system should consider risk factors and other noninvasive tests in addition to ABI to develop and ideal screening system for CA. (Clinical trial registration number NCT04667832)
Coronaviruses with a SARS-CoV-2-like receptor-binding domain allowing ACE2-mediated entry into human cells isolated from bats of Indochinese peninsula
Authors: Temmam Sarah , Vongphayloth Khamsing , Salazar Eduard Baquero , Munier Sandie , Bonomi Max , Régnault Béatrice , Douangboubpha Bounsavane , Karami Yasaman , Chretien Delphine , Sanamxay Daosavanh , Xayaphet Vilakhan , Paphaphanh Phetphoumin , Lacoste Vincent , Somlor Somphavanh , Lakeomany Khaithong , Phommavanh Nothasin , Pérot Philippe , Donati Flora , Bigot Thomas , Nilges Michael , Rey Félix , Werf Sylvie van der , Brey Paul , Eloit Marc
Published: September 2021

The animal reservoir of SARS-CoV-2 is unknown despite reports of various SARS-CoV-2-related viruses in Asian Rhinolophus bats, including the closest virus from R. affinis, RaTG13. Several studies have suggested the involvement of pangolin coronaviruses in SARS-CoV-2 emergence. SARS-CoV-2 presents a mosaic genome, to which different progenitors contribute. The spike sequence determines the binding affinity and accessibility of its receptor-binding domain (RBD) to the cellular angiotensin-converting enzyme 2 (ACE2) receptor and is responsible for host range. SARS-CoV-2 progenitor bat viruses genetically close to SARS-CoV-2 and able to enter human cells through a human ACE2 pathway have not yet been identified, though they would be key in understanding the origin of the epidemics. Here we show that such viruses indeed circulate in cave bats living in the limestone karstic terrain in North Laos, within the Indochinese peninsula. We found that the RBDs of these viruses differ from that of SARS-CoV-2 by only one or two residues, bind as efficiently to the hACE2 protein as the SARS-CoV-2 Wuhan strain isolated in early human cases, and mediate hACE2-dependent entry into human cells, which is inhibited by antibodies neutralizing SARS-CoV-2. None of these bat viruses harbors a furin cleavage site in the spike. Our findings therefore indicate that bat-borne SARS-CoV-2-like viruses potentially infectious for humans circulate in Rhinolophus spp. in the Indochinese peninsula.
SENP3 Promotes Bladder Cancer Proliferation and EMT Transformation by Affecting the Expression of PYCR1 via DeSUMOylation of STAT3
Authors: Li Zhuo , Liu Jian , Fu Huifeng , Li Yuanwei , Lu Qaing , Song Wei , Zeng Mingqiang
Published: September 2021

Abnormal activation of signal transducer and activator of transcription 3 (STAT3) has been found in various types of human cancers, including bladder cancer (BC). In our study, we examined the regulation of STAT3 post-translational modifications (PTMs) and found that SENP3 is high in bladder cancer. SENP3 and STAT3 were highly expressed in BC tissues when compared with tissue adjacent to carcinoma. SENP3 induced STAT3 protein level and p-STAT3 translocating into nuclear through deSUMOylation of STAT3. Further, nuclear STAT3, as a transcriptional activity factor, promoted pyrroline-5-carboxylate reductase 1 PYCR1 gene and protein level by interacting with the promoter of (PYCR1). Next, we found that knockdown of PYCR1 inhibited Epithelial to mesenchymal transition of bladder cancer, and simultaneously mitigated the carcinogenic effects of STAT3. In vitro, STAT3 knockdown in bladder cancer cells inhibited cell proliferation, migration, and invasion. In contrast, SENP3 overexpression reversed these effects. In all, results lend novel insights into the regulation of STAT3, which has key roles in bladder cancer progression.
Effect of Dihydropyridine Calcium-Channel Blocker on Adverse Aortic Events After Thoracic Endovascular Aortic Repair for Type B Aortic Dissection
Authors: Yang Fan , Chen Lyufan , Liu Jitao , Luo Songyuan , He Caiyun , Ma Wentao , Xie Enmin , Yang Xinyue , Zeng Hongke , Geng Qingshan , Luo Jianfang
Published: September 2021

Objectives: This study aimed to evaluate the effect of dihydropyridine calcium-channel blocker (CCB) on adverse aortic events (AAE) in patients undergoing thoracic endovascular aortic repair (TEVAR) for type B aortic dissection (TBAD). Methods: A retrospective study was conducted on patients undergoing TEVAR of TBAD from January 2010 to December 2017 in our center. Patients were divided into CCB group and non-CCB group according to the postoperative dihydropyridine CCB use. AAE including aorta-relative mortality and reintervention, were compared between these two groups. Propensity score matching analysis was performed to avoid the potential effect of known confounders.Results: Before matching, the study cohort comprised 548 patients, including 435 (79.4%) and 113 (20.6%) patients in the CCB and non-CCB groups, respectively. After matching, 101 patients in each group were eligible for the analysis. In the unmatched cohort, AAE occurred in 52 (12.0%) and 29 (25.7%) patients in the CCB and non-CCB groups, respectively (P < 0.001). In the matched cohort, AAE occurred in 8 (7.9%) and 22 (21.8%) patients in the CCB and non-CCB groups, respectively (P = 0.013). Log-rank test analysis revealed that the levels of freedom from AAE were significantly different between the 2 groups in both the unmatched and matched cohorts (P < 0.001 and P = 0.007, respectively). Multivariable analysis showed that CCB use (hazard ratio 0.50, 95% confidence interval 0.32–0.80; P = 0.003) was associated with a lower AAE rate after adjustment for other variables, and was minimally changed after the propensity score matching (HR 0.34, 95% CI 0.15–0.75; P = 0.008).Conclusions: Postoperative dihydropyridine CCB use is protective in patients undergoing TEVAR for acute and sub-acute TBAD.
Fault diagnosis method of mine hoist braking system based on deep convolution neural network
Authors: Yan Fangyuan , Li Juanli , Miao Dong , Cao Qi
Published: September 2021

A reliable braking system is an important guarantee for safe operation of mine hoist. In order to make full use of the monitoring data in the operation process of mine hoist, identify the operation status of the hoist, and further carry out fault diagnosis on it, the deep learning method was introduced into the fault diagnosis of the hoist, and a fault diagnosis method of hoist braking system based on convolution neural network has been proposed. Firstly, the working principle and fault mechanism of disc brake and its hydraulic station in hoist braking system are analyzed, and the monitoring parameters of this study are determined; then, based on massive monitoring data, the convolutional neural networks (CNN) is established, the one-dimensional signal collected by the sensor is transformed into two-dimensional image for coding, the neural network is trained by gradient descent method, and the network structure parameters are modified according to the training results. Finally, the fault diagnosis model is compared and verified by using the sample set based on the traditional back propagation neural network (BP) and CNN. The results show that the accuracy of CNN is higher than that of BP, and the accuracy rate can reach 99.375% after reducing the involvement between samples. This method can make full use of the monitoring data for diagnosis, without subjective intervention of experts, and improve the accuracy of diagnosis.
Hyperhomocysteinemia Is Associated With Lipid Profiles and Lipid Ratio in Patients With Coronary Artery Disease
Authors: Wu Dong-Feng , Liao Qin-Chen , Lu Feng , Wang Zhou , Yu Kun , Deng Jin-Long
Published: September 2021

Objective: This study aimed to investigate the correlation of Hyperhomocysteinemia (HHcy) or serum homocysteine (Hcy) levels with lipid levels and lipid ratios in individuals with coronary artery disease(CAD).Methods: A total of 1646 subjects with suspected CAD were divided into CAD or control groups. Serum Hcy, total cholesterol(TC), triglycerides(TGs), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), apolipoprotein(Apo)AI and ApoB concentrations were detected.Results: Serum TC, LDL-C and ApoB in control subjects with HHcy were lower than those in individuals with normal Hcy, and serum HDL-C and ApoAI in CAD subjects with HHcy were lower than those in individuals with normal Hcy(P < 0.05). The correlation analysis showed that serum TGs, LDL-C, ApoAI and HDL-C were correlated with Hcy(P <0.05). There are different HHcy trends affecting the ratios of TC/HDL-C and LDL/HDL-C between the CAD and controls(Pinteraction for TC/HDL-C=0.025; Pinteraction for LDL/HDL-C=0.033). CAD patients with HHcy had a higher ratio of TC/HDL-C(P=0.022) and LDL/HDL-C(P=0.045) than those with normal Hcy, but in the controls, the subjects with HHcy exhibited a trend toward a decreased ratio of TC/HDL-C(P=0.481) and LDL/HDL-C(P=0.303).Conclusion: HHcy was related to the atherogenic lipid profile in patients with CAD. The lipid ratio is more suitable for assessing the effect of HHcy on CAD.
Reduced Regime of Glucocorticoids and Cyclophosphamide for Mild ANCA-Associated Renal Vasculitis May Delay Deterioration of Renal Function
Authors: Li Ziyang , Bao Jiwen , Yao Huanzhen , Wang Qin , Zhang Minfang , Gu Leyi , Ni Zhaohui , Wang Ling
Published: September 2021

ObjectivesTo compare the efficacy and safety of initial reduced-dose glucocorticoids combined with reduced-frequency cyclophosphamide and to determine risk predictors of end-stage renal disease in ANCA-associated vasculitis（AAV）patients with renal involvement which BVAS was less than 20.MethodsThis is a single-center retrospective cohort study that involved 58 patients who were newly diagnosed with ANCA-associated renal vasculitis. The efficacy and safety of reduced-frequency cyclophosphamide combined with initial reduced-dose glucocorticoids were compared using chi-square test. The cumulative probability to ESRD were estimated using the Kaplan–Meier method and compared using the log rank test. Potential variates were examined using multivariate Cox proportional hazard models to determine the risk predictors of end-stage renal disease.ResultsA total of 35 patients in the standard-dose glucocorticoids group and 23 in the reduced-dose glucocorticoids group were included. The average age of the included patients was 62.45±12.70 years, and the baseline serum creatinine was 251.35[155.53, 445] μmol/L. Nine patients (15.52%) developed ESRD within 24 months (7 Standard vs. 2 Reduced, P=0.035). Multivariate Cox regression model analysis proved that baseline serum creatinine (HR: 0.007, 95%CI: 2.48-39.48, P=0.014), infection rate within first 3 months (HR: 2.28, 95% CI: 2.14 45.27, P=0.003), persistent hematuria for more than 6 months (HR: 1.723, 95%CI: 0.043-0.738, P=0.017) were risk predictors of end-stage renal disease in ANCA-associated renal vasculitis.ConclusionThe regime of initial reduced-dose glucocorticoids combined with reduced-frequency cyclophosphamide is not inferior to the standard regimen in AAV patients with renal involvement which BVAS score was less than 20，and meantime the incidence of infection was significantly lowered. Patients had infection within first 3 months were at higher risk in development of ESRD. To reduce the incidence of infection and thus delay deterioration of renal function, the reduced-dose regime may be more appropriate than standard-dose regimen and can be used as an option in mild patients.
A semi-analytical elastic stress solution for circular rock stratum based on the theory of curved beam
Authors: BU wankui , XU Hui
Published: September 2021

The circular rock stratum are inevitably encountered in underground mining engineering, which causes waste of resources and dynamic instability. Based on the elastic theory of curved beam and finite-difference computation, a displacement function is presented in polar coordinates for solving two partial differential equations with the boundary conditions in mixed type in elastic, isotropic and homogeneous rock. A semi-analytical elastic stress solution for circular rock stratum is obtained according to the governing equation and stress components in terms of displacement function. The variations of stress distribution with different influencing factors are analyzed, which may lead to a better understanding of the stability of circular rock stratum after coal extractions. Finally, this semi-analytical elastic stress solution is applied to the fold structure in No.2502 mining area and points out the dangerous sites during coal extractions, which provides the basis of construction and safety in coal mine engineering.
Characterization of The Physicochemical Properties, Antioxidant Activity, And Antiproliferative Activity of Natural Melanin From S. Reiliana
Authors: Lu Ming , Xie Mengxi , Fu Xin , Yu Miao , Shi Taiyuan
Published: September 2021

This study aimed to characterize the physicochemical properties and stability of L-25 melanin extracted from Sporisorium reilianum (S. reiliana). The results showed that the maximum absorption wavelength of melanin was 215 nm. Reducing agents, heat, light, microwaving, oxidants, and common food additives did not affect the melanin. Additionally, it has a good metal stability except Mn2+. The IR spectra revealed the presence of O-H, N-H, C=O, and C=C bonds as well as carboxyl, alcohol hydroxyl, and phenolic hydroxyl groups and a pyran ring. L-25 melanin could be defined as DL-hydroxy phenylalanine (DOPA)-melanin. The antioxidant and antiproliferative were also measured. The melanin has a specific stability and high antioxidant activity, including a strong DPPH free radical scavenging ability, and protected damaged HepG2 cells by reducing reactive oxygen species, malondialdehyde, and lactate dehydrogenase content. In conclusion, S. reilianum represents a novel source of melanin, that could be applied to health food or food additives. Our results show that melanin from S. reilianum is a natural pigment with good stability that has a great prospect of development and application, providing a theoretical basis and methods for its further processing and development as a functional food.
Glecaprevir/ Pibrentasvir in the Treatment of Chronic Hepatitis C Patients – A Real-World Nationwide HCV Registry Program (TACR) in Taiwan
Authors: Huang Chung-Feng , Kuo Hsing-Tao , Chang Te-Sheng , Lo Ching-Chu , Hung Chao-Hung , Huang Chien-Wei , Chong Lee-Won , Cheng Pin-Nan , Yeh Ming-Lun , Peng Cheng-Yuan , Cheng Chien-Yu , Huang Jee-Fu , Bair Ming-Jong , Lin Chih-Lang , Yang Chi-Chieh , Wang Szu-Jen , Hsieh Tsai-Yuan , Lee Tzong-Hsi , Lee Pei-Lun , Wu Wen-Chih , Lin Chih-Lin , Su Wei-Wen , Yang Sheng-Shun , Wang Chia-Chi , Hu Jui-Ting , Mo Lein-Ray , Chen Chun-Ting , Huang Yi-Hsiang , Chang Chun-Chao , Huang Chia-Sheng , Chen Guei-Ying , Kao Chien-Neng , Tai Chi-Ming , Liu Chun-Jen , Lee Mei-Hsuan , Tsai Pei-Chien , Dai Chia-Yen , Kao Jia-Horng , Lin Han-Chieh , Chuang Wang-Long , Chen Chi-Yi , Tseng Kuo-Chih , Yu Ming-Lung
Published: September 2021

Background/AimsThe study evaluated the real-world treatment outcomes of Glecaprevir/pibrentasvir (GLE/PIB) including effectiveness, safety and healthcare resource utilization based on a nation-wide registry in Taiwan.MethodsThe Taiwan HCV Registry (TACR) is a nation-wide platform organized and supervised by the Taiwan Association for the Study of the Liver. Data were analyzed for patients treated with GLE/PIB, including 3,144 patients who had treatment outcome available. The primary endpoint was sustained virological response (SVR12, undetectable HCV RNA throughout 12 weeks of end-of-treatment). ResultsThe overall SVR12 rate was 98.9% (3110/3144), with 98.8%, 99.4% and 100% in patients receiving 8 weeks, 12 weeks, and 16 weeks of GLE/PIB respectively. The SVR12 rate in the treatment-naïve cirrhotic patients receiving 8 weeks of GLE/PIB was 98.2% (108/110). The most common AEs were fatigue (7.5%), pruritus (6.7%) and dizziness (1.5%). The mean number of outpatient visits during the GLE/PIB was 5.94 visits for patients treated with 8 weeks, significantly different from the patients treated with 12 weeks of GLE/PIB (6.90 visits). ConclusionsThe results support the effectiveness and safety of GLE/PIB treatment in real-world clinical practice, and provide further evidence that the shorter, 8-week GLE/PIB regimen is effective and cost-saving.
Risk Factor Analysis of Intravesical Recurrence After Laparoscopic Nephroureterectomy for Upper Tract Urothelial Carcinoma
Authors: Yanagi Masato , Hamasaki Tsutomu , Akatsuka JunJun , Endo Yuki , Takeda Hayato , Kondo Yukihiro
Published: September 2021

Background: One of the major concerns of patients with upper tract urothelial carcinoma (UTUC) treated with nephroureterectomy is intravesical recurrence (IVR). The purpose of the present study was to investigate the predictive risk factors for IVR after laparoscopic nephroureterectomy (LNU) for UTUC.Methods: Clinicopathological and surgical information were collected from the medical records of 73 patients treated with LNU for non-metastatic UTUC, without a history of or concomitant bladder cancer. The association between IVR after LNU and clinicopathological and surgery-related factors, including preoperative urine cytology and pneumoperitoneum time, was analyzed using Cox proportional hazards regression models and the Kaplan–Meier method with log-rank test.Results: During the median follow-up time of 39.1 months, 18 (24.7%) patients had subsequent IVR after LNU. The 3- and 5-year IVR-free survival rates were 76.5% and 74.3%, respectively. In the multivariate Cox regression analysis, positive preoperative urine cytology (hazard ratio [HR]: 3.55; 95% confidence interval [CI]: 1.326–11.327; p=0.011) and prolonged pneumoperitoneum time of ≥ 210 min (HR: 3.40; 95% CI: 1.271–10.692; p=0.014) were independent prognostic factors for IVR-free survival. In patients with positive urine cytology, the Kaplan–Meier method with log-rank test revealed that the 3-year and 5-years IVR free survival rates were 46.3% and 39.7%, respectively, in patients with a prolonged pneumoperitoneum time of ≥ 210 min, which was significantly lower than that in their counterparts (76% and 76%, respectively, p=0.041).Conclusions: In UTUC patients with positive urine cytology, the occurrence of IVR is highly probable when the pneumoperitoneum time of LNU is prolonged (≥ 210 min). Strict follow-up after LNU is highly recommended for these patients.
Financial Burden and Health-seeking Behaviors Related to Chronic Diseases Under the National Health Insurance Scheme in Bolikhamxay Province, Lao PDR: a Cross-sectional Study
Authors: Ito Tomoo , Miyoshi Chiaki , Kounnavong Sengchanh
Published: September 2021

Background: Financial protection is a key dimension of universal health coverage. In 2016, Lao PDR implemented the National Health Insurance system covering the entire population of certain provinces. This cross-sectional study investigated households’ health-seeking behavior and their financial burden with chronic patients, post coverage. Method: The study was conducted in Bolikhamxay province from January 15 to February 13, 2019. In total, 487 households, selected via stratified random sampling, were surveyed, and questionnaire-based interviews conducted. Health care service utilization and financial burden were examined.Results: Totally, 370 households had at least one member with some type of self-reported health problem within the last 3 months prior to the interview, while 170 had at least one member with a chronic condition. More than 75% of the households accessed a health facility when a member experienced health problems. We observed that the prevalence of catastrophic health expenditure (health expenditure/income between 20% and 40%) was 25.1% (threshold of 20%) and 16.2% (threshold of 40%). Through logistic regression, we found that the major factors determining financial catastrophes owing to health problems were household members with chronic illness, hospitalization, household poverty status, family size (both 20% and 40% thresholds), visiting a private facility (20% threshold), and distance from provincial top referral hospital (40% threshold).Conclusions: The National Health Insurance system has positively impacted households’ access to health facilities. However, catastrophic health expenditure remains high, especially among chronic patients. Facilities under National Health Insurance should be strengthened to provide more services, including care for chronic conditions.
Optimizing Hybrid Metaheuristic Algorithm with Cluster Head to Improve Performance Metrics on the IoT
Authors: alazzam Malik bader , Alassery Fawaz
Published: September 2021

The Internet of Things (IoT) has subsequently been applied to a variety of sectors, including smart grids, farming, weather prediction, power generation, wastewater treatment, and so on. So if the Internet of Things has enormous promise in a wide range of applications, there still are certain areas where it may be improved. Designers had focused our present research on reducing the energy consumption of devices in IoT networks, which will result in a longer network lifetime. The far more suitable Cluster Head (CH) throughout the IoT system is determined in this study to optimize energy consumption. Whale Optimization Algorithm (WOA) with Evolutionary Algorithm (EA) is indeed a mixed meta-heuristic algorithm used during the suggested study. Various quantifiable metrics, including the variety of adult nodes, workload, temperatures, remaining energy, and a target value, were utilized IoT network groups. The suggested method then is contrasted to several cutting-edge optimization techniques, including the Artificial Bee Colony method, Neural Network, Adapted Gravity Simulated annealing. The findings show that the suggested hybrid method outperforms conventional methods.
Identification and characterization of a novel potyvirus infecting Paris yunnanensis
Authors: Lan Pingxiu , He Peng , Cao Mengji , Zhou Guohua , Chenrong Li , Tan Guanlin , Chen Xiaojiao , Yang Jie , Wei Taiyun , Li Fan
Published: September 2021

The complete genomic sequence of a novel potyvirus from Paris yunnanensis was determined by high-throughput sequencing then confirmed by Sanger sequencing. Its genomic RNA consists 9600 nucleotides (nt) excluding the 3’-terminal poly (A) tail, containing a typical large open reading frame (ORF) of potyviruses and encoding a putative polyprotein of 3098 amino acids (aa). Pairwise comparison analysis showed the virus shares sequence identity with other members of Potyvirus was 53.0–57.8% at genome sequence level, and 39.3–51.2% at polyprotein sequence level. Phylogenetic analysis indicated that the virus was clustered as a single clade within the genus Potyvirus both using nt and aa level. These results suggest that the virus should be considered as a distinct species within the genus Potyvirus, and it was tentatively named as “Paris mottle virus” (PaMoV).