bluuebunny/crossref_metadata_2025 huggingface dataset:
The effects of weight loss and improved metabolic health status on the risk of non-alcoholic fatty liver disease—results from a prospective cohort in China
Authors: Huang Xin , Ouyang Wenbin , Hu Yang , Tang Bei , He Yongmei , Wu Hao , Yang Pingting , Yin Lu , Liu Qingqi , Chen Kui , Deng Jing , Li Xiaohui , Li Ying
Published: November 2023

Background

The impact of weight loss and/or improved metabolic status on the risk of non-alcoholic fatty liver disease (NAFLD) has yet to be determined.

Methods

A total of 35,322 participants without NAFLD were followed. NAFLD risk was compared between consistently metabolically healthy non-obese (MHNO) and non-MHNO who lost weight to become non-obese and/or improved their metabolic health, using Cox proportional hazards and logistic regression models.

Results

Following 148,186 person-years, 8,409 participants had onset NAFLD, with an incidence rate of 56.75 (95% CI: 55.57, 57.94) per 1,000 person-years. Metabolically healthy obese (MHO), metabolically unhealthy obese (MUO), and metabolically unhealthy non-obese (MUNO) at baseline were associated with increased NAFLD risk, with hazard ratios of 4.48 (95%CI:4.24, 4.73), 8.85 (95%CI:7.95, 9.84), and 10.70 (95%CI:9.73, 11.78). Weight loss and/or metabolic status improvements could significantly reduce NAFLD risk by 79.46 to 41.46%. Specifically, after weight loss from MHO to MHNO, the reduction in NAFLD risk [OR decreased from 12.01 (95%CI:9.40, 15.35) to 4.14 (95%CI:3.08, 5.57)] was greater than that of the MUNO subgroup whose metabolic status improved to MHNO [OR decreased from 5.53 (95%CI:5.15, 5.94) to 2.71 (95%CI:2.50, 3.93)]. In the MUO subgroup, the group with the greatest risk reduction of NAFLD was the weight and metabolic state both improvement group [MUO to MHNO, OR decreased from 22.74 (95%CI:17.61, 29.37) to 4.67 (95%CI:3.05, 7.16)], followed by the weight loss only group [MUO to MUNO, OR decreased to 6.83 (95%CI:4.87, 9.57)], and finally the group with the least and insignificant risk reduction was the metabolic state improvement group [MUO to MHO, OR decreased to 13.38 (95%CI:9.17,19.53)]. NAFLD risk was negatively correlated with the duration of improvement (p < 0.001).

Conclusion

Individuals with non-MHNO were more likely to develop NAFLD than those with consistent MHNO, but metabolic improvements and weight loss can alleviate the risk. Their NAFLD risk was negatively correlated with improvement duration. However, it remained higher than in individuals with consistent MHNO at an average follow-up of 4.2 years.
Heterogeneity matching and IDH prediction in adult-type diffuse gliomas: a DKI-based habitat analysis
Authors: Liu Yanhao , Wang Peng , Wang Shaoyu , Zhang Huapeng , Song Yang , Yan Xu , Gao Yang
Published: November 2023

Objective

To explain adult-type diffuse gliomas heterogeneity through diffusion kurtosis imaging-based habitat characteristics and develop and validate a comprehensive model for predicting isocitrate dehydrogenase (IDH) status.

Materials and methods

In this prospective secondary analysis, 103 participants (mean age, 52 years; range, 21-77; 54 [52%] male) pathologically diagnosed with adult-type diffuse gliomas were enrolled between June 2018 and February 2022. The Otsu method was used to generate habitat maps with mean diffusivity (MD) and mean kurtosis (MK) for a total of 4 subhabitats containing 16 habitat features. Habitat heatmaps were created based on the Pearson correlation coefficient. The Habitat imAging aNd clinicraD INtegrated prEdiction SyStem (HANDINESS) was created by combining clinical features, conventional MRI morphological features, and habitat image features. ROC, calibration curve, and decision curve analyses were used to select the optimal model after 32 pipelines for model training and validation.

Results

In the restricted diffusion and high-density subhabitat, MK was highly correlated with MD (R2 = 0.999), volume (0.608) and percentage of volume (0.663), and this region had the highest MK value (P<.001). The unrestricted diffusion and low-density subhabitat had the highest MD value (P<.001). When MK was less than the Otsu threshold, there was still a difference between restricted diffusion and low-density and unrestricted diffusion and low-density subhabitats (P<.01). The HANDINESS enabled more accurate prediction of the IDH status in the training (AUC=0.951 [0.902-0.987]) and internal validation cohorts (0.938 [0.881-0.949]). AUC values for single-modality models and independent factors ranged from 0.593 to 0.916. Calibration and decision curve analyses showed that the HANDINESS demonstrated a high level of clinical applicability and predictive consistency.

Conclusion

Diffusion kurtosis imaging-based habitat analysis provides additional important information on microscopic tumor spatial heterogeneity. The HANDINESS has higher diagnostic performance and robustness than single-modality models.
Identifying modifiable factors associated with COVID-19 vaccine hesitancy and acceptance among cancer patients in Jordan
Authors: AlMasri Rama , Al Masri Mahmoud , Darwish Rula , Ammar Khawla , Safi Yasmin
Published: November 2023

Introduction

Vaccines stand amongst the most effective medical interventions for the management of infectious diseases, and are pivotal tools for public health. The acceptance of vaccines is heavily influenced by perceptions of efficacy, safety and other modifiable factors.

Purpose

This cross-sectional study sought to identify and examine the modifiable factors that can help address COVID-19 vaccine hesitancy and acceptance among cancer patients.

Methods

The study was conducted between February and April 2021 using an online survey questionnaire comprising of four domains. The survey was administered to cancer patients in Jordan.

Results

Among the 1,029 cancer patients who completed the online questionnaire (response rate= 73%), 58% (n=597) expressed willingness (intent) to take the vaccine. Notably, 72.5% (n=433) of those intending to take the vaccine were currently undergoing treatment. Knowledge and awareness played a significant role, with 54.3% considering them essential for vaccine acceptance. Fear of infection significantly influenced vaccine acceptance (p<0.001), with 66.8% expressing concern about potential infections. Peer encouragement was also a crucial factor, as 82.4% regarded it as an important driver for influencing vaccine acceptance (p<0.001).

Conclusion

Peer encouragement, awareness, and fear emerged as the primary modifiable factors associated with greater vaccine acceptance by patients with active malignancies. Study results suggest that providing personalized and tailored information about vaccinations, focusing on safety and potential interactions with cancer and its treatment, are potentially excellent strategies for improving vaccine acceptance among cancer patients.
Case Report: Gastrointestinal neuroendocrine carcinoma with SMARCA4 deficiency: a clinicopathological report of two rare cases
Authors: Zhou Ping , Fu Yiyun , Wang Weiya
Published: November 2023

Background

Gastrointestinal neuroendocrine carcinoma (GI NEC) is a rare but highly malignant neoplasm with an aggressive clinical course. SMARCA4 is one of the subunits of the SWI/SNF chromatin remodeling complex. SMARCA4 deficiency can occur rarely in subsets of NECs. Reports of the clinicopathological features of GI NECs with SMARCA4 deficiency are limited.

Methods

In this study, we retrospectively reported two rare cases of GI NEC with SMARCA4 deficiency and described the clinicopathological, radiographic and histopathological features.

Results

Case 1 was a 43-year-old male with a stage cT3NxM1, IV tumor. Case 2 was a 64-year-old female with a stage cT4aN1M0, IIIA tumor. Both tumors presented as ulcerated masses with infiltration. Pathological examination indicated a solid architecture with poorly differentiated morphology, and complete loss of SMARCA4 (BRG1) was found. Immunohistochemical staining showed positivity for Syn, CgA and CD56. The Ki-67 index was 90% and 70%, respectively. None of the cases had mismatch repair (MMR) deficiency. Case 1 received treatment with chemotherapy and anti-PD-1 immunotherapy. He did not respond to treatment, and died 9 months later. Case 2 received neoadjuvant chemotherapy before surgical treatment, and the tumor showed TRG3 in response to neoadjuvant chemotherapy, chemotherapy and anti-PD-1 immunotherapy were continued after surgical resection. There was no evidence of disease for 10 months.

Conclusions

GI NEC with SMARCA4 deficiency is a rare entity of gastric NEC. SMARCA4 may be a promising targetable and prognostic biomarker. BRG1 immunohistochemical staining could be performed for GI NECs. Further studies with a larger cohort will be needed.
Integrating treatment cost reduction strategies and biomarker research to reduce costs and personalize expensive treatments: an example of a self-funding trial in non-small cell lung cancer
Authors: Buma Alessandra I. G. , Piet Berber , ter Heine Rob , van den Heuvel Michel M.
Published: November 2023

Personalization of treatment offers the opportunity to treat patients more effectively based on their dominant disease-specific features. The increasing number and types of treatment, and the high costs associated with these treatments, however, demand new approaches that improve patient selection while reducing treatment-associated costs to ensure sustainable healthcare. The DEDICATION-1 trial has been designed to investigate the non-inferiority of lower dosing regimens when compared to standard of care dosing regimens as a potential effective treatment cost reduction strategy to reduce costs of treatment with expensive immune checkpoint inhibitors in non-small cell lung cancer. If non-inferiority is confirmed, lower dosing regimens could be implemented for all therapeutic indications of pembrolizumab. The cost savings obtained within the trial are partly reinvested in biomarker research to improve the personalization of pembrolizumab treatment. The implementation of these biomarkers will potentially lead to additional cost savings by preventing ineffective pembrolizumab exposure, thereby further reducing the financial pressure on healthcare systems. The concepts discussed within this perspective can be applied both to other anticancer agents, as well as to treatments prescribed outside the oncology field.
Pulse shaping, high average power narrow linewidth Er, Yb co-doped fiber amplifier
Authors: Wang Yunpeng , Li Yang , Zhang Dongming , Xiong Chen , Li Pingxue
Published: November 2023

We demonstrate a high repetition rate, high average power narrow-linewidth fiber amplifier at 1.55 μm eye-safe waveband by using a very-large-mode-area active fiber doping Er and Yb, having a 60-μm-diameter core and a 600-μm-diameter inner cladding. A maximum average power reaches 16 W at a 10 kHz repetition rate for 600 ns pulses in the stimulated Brillouin scattering (SBS) free operation, which calculates to a 1.6 mJ pulse energy and 2.7 kW peak power. In addition, the peak power rises to 4.9 kW with 148 ns pulses at the onset of SBS. The signal peak is located at 1,551.15 nm with a 36 dB signal-to-noise ratio (SNR). In order to eliminate the pulse steepening, the pulse shaping technology using a triangular-like wave with a slower rising edge is applied on the input pulses. The half-width of the frequency-intensity spectrum from high-average-power fiber amplifier is about 1.02 MHz, approaching the Fourier transform limit.
Perceived marginalization and mental health of young adults with migration backgrounds in South Korea: exploring moderating and mediating mechanisms
Authors: Jang Sojung , Ryu Jiwon , Yon Kyu Jin , Kim Paul Youngbin , Kim Min Sun
Published: November 2023

This study explored the mediating effect of career maturity moderated by intimacy with parents and immigration backgrounds (native- or foreign-born young adults) on the relationship between perceived marginalization and the mental health of young adults with migration backgrounds (having mixed parentage of one Korean and one non-Korean immigrant parent) in South Korea. We collected data from 300 adults aged 25–34 with migration backgrounds (204 born in Korea and 96 born abroad) through the Gallup Research Institute of Korea and conducted a moderated-moderated mediation analysis using Model 21 of PROCESS Macro in SPSS. The analysis showed that career maturity moderated by intimacy with parents and migration backgrounds mediated the relationship between perceived marginalization and mental health. However, the results were only significant for participants who were born abroad and immigrated to Korea, and not for those who were born in Korea. These findings suggest that while greater perceived marginalization leads to lower career maturity and negatively impacts the mental health of foreign-born young adults, higher levels of intimacy with parents can buffer these negative effects.
The psychology of romantic relationships: motivations and mate preferences
Authors: Tartakovsky Eugene
Published: November 2023

Introduction

This study investigates motivations to engage in romantic relationships. We examine the structure of romantic motivations and their connections with personal values and mate preferences.

Method

The study was conducted in Israel among young men and women looking for a romantic partner (n = 1,121, 40% male, age 18–30).

Results

Data analysis demonstrated that basic romantic motivations form a circumplex that may be partitioned into four higher-order romantic motivations: love and care, family and children, status and resources, and sex and adventure. The romantic motivations formed a meaningful pattern of connections with higher-order values, thus confirming that context-specific motivations are derived from general motivational goals expressed in values. Personal value preferences and romantic motivations predicted the sought-after partner characteristics over and above sociodemographic variables. Values were indirectly (through romantic motivations) and directly connected to mate preferences.

Discussion

The study advances our understanding of romantic relationships among young people and opens new directions for research and counseling.
Overcoming treatment gaps in the management of depression with non-pharmacological adjunctive strategies
Authors: Purebl György , Schnitzspahn Katharina , Zsák Éva
Published: November 2023

There is considerable evidence that simple, cost-effective, non-pharmaceutical strategies can be readily implemented to improve outcomes in the treatment of depression. It is estimated that 4.4% of the world’s population suffers from depression. Despite being a major public health concern and the availability of both pharmacological and non-pharmacological treatments, many depressed people remain undiagnosed and receive no or inappropriate treatment. Several possible underlying factor of treatment gap can be identified in relation to pharmacotherapy and psychotherapy of depression, including side effects, partial remission, treatment-resistant depression and the limited availability of psychotherapy. In addition to developing new therapeutic options, much more could be done to optimise the use of existing therapies, including combining available drug treatments with quick, simple and cost-effective non-pharmacological methods: low-intensity psychological interventions, online self-help tools and lifestyle medicine. In addition to increasing the effectiveness of treatments, prevention is equally important: awareness programs to further reduce the treatment gap, and community dissemination of the life skills that help maintain positive mental health.
Influence of different caregiving styles on fundamental movement skills among children
Authors: Hu Jiahui , Zhang Shudan , Ye Weibing , Zhu Yuanye , Zhou Huiling , Lu Lihua , Chen Qian , Korivi Mallikarjuna
Published: November 2023

Purpose

This study investigated the influence of parenting and grandparenting caregiving styles on fundamental motor skills (FMS) of preschool children.

Method

A total of 1,326 preschool children (698 boys, 628 girls) aged 4–6 years were recruited from the kindergartens of Jinhua City, China. Locomotor skills (LM), ball skills (BS), and total fundamental movement skills (TS) of children were assessed by the Test of Gross Motor Development-3rd edition (TGMD-3).

Results

There were 978 children in parenting and 348 children in grandparenting caregiving styles. The LM, BS and TS scores of children were considerably (p < 0.001) increased with age (irrespective of sex or caregiving style). For the sex comparisons, BS scores of boys were significantly higher than girls (p < 0.001), while LM and TS scores were not different between boys and girls. For the caregiving style comparison, parenting is superior to grandparenting in developing of children's FMS. Parenting boys of 4-, 5-, and 6-years old showed better BS compared to age-matched parenting girls, whereas boys of 5-years old in grandparenting only showed better BS compared to same-age grandparenting girls (p < 0.05). Furthermore, parenting boys of 6-years reported higher LM (p < 0.01), BS (p < 0.001), and TS (p < 0.001) scores compared to grandparenting boys, but girls' FMS at all ages were not significantly different between the caregiving styles.

Conclusion

Parenting caregiving style is positively associated with proper development of FMS among children. Girl children with poor FMS in grandparenting may need a special care or intervention programs to promote their FMS.
Insights from China: understanding the impact of community resilience and government trust in psychological resilience and anxiety during COVID-19
Authors: Hu Yue , Huang Yuxin , Zhang Hua , Fang Min , Chen Guobang
Published: November 2023

Background

COVID-19 has the potential to greatly impact an individual mental well-being. However, an individual’s psychological resilience, combined with support from their community and government disaster relief efforts can aid individuals in confronting crises with a positive mindset. The purpose of this study is to investigate how individuals, across three dimensions of individual resilience perception, community resilience perception, and government trust perception, mitigate individual anxiety during COVID-19.

Methods

This study employed an online survey method that was not restricted by geographical location. Data collection took place from January 2022 to June 2022, and the valid questionnaires covered all 31 provinces, autonomous regions, and municipalities in China. The assessment of community resilience was conducted employing the Conjoint Community Resilience Assessment Measure-10 (CCRAM-10). Structural Equation Modeling (SEM) was also used to examine the relationship between community resilience, government trust, individual psychological resilience, and anxiety.

Results

The SEM results reveal that individual psychological resilience is significantly negatively correlated with anxiety (b = −0.099, p < 0.001), while there is a significant positive correlation between community resilience perception (b = 0.403, p < 0.001) and government trust (b = 0.364, p < 0.001) with individual psychological resilience. Furthermore, government trust perception enhances psychological resilience, consequently reducing anxiety (b = −0.036, p < 0.001). The results also revealed that women and increasing age had a mitigating effect on individual anxiety during COVID-19.

Conclusion

Individual’s mental state is influenced on multiple dimensions during COVID-19. Not only can individual psychological resilience better cope with anxiety, but support at the community and government dimensions has a significant impact on individual psychology. These resources can enhance the resilience of both individuals and communities, helping them better cope with stress and difficulties.
Hazardous, harmful, and dependent alcohol use in healthcare professionals: a systematic review and meta-analysis
Authors: Halsall Lauren , Irizar Patricia , Burton Sam , Waring Sara , Giles Susan , Goodwin Laura , Jones Andrew
Published: November 2023

Background

Healthcare professionals work in high-pressured and demanding environments, which has been linked to the use of alcohol as a coping strategy. This international review aimed (i) to determine the pooled prevalence of hazardous, harmful, dependent, and frequent binge drinking in healthcare professionals, and (ii) to explore factors associated with variation in these outcomes.

Methods

Scopus, MEDLINE, and PsycINFO were searched from 2003 to 17th November 2022, for studies reporting a prevalence estimate for any outcome among healthcare professionals. Random-effects meta-analyses determined pooled prevalence estimates. Sub-group analyses were conducted, stratifying the meta-analyses by pandemic period vs pre-pandemic period. Meta-regressions explored factors that were associated with variation in the outcomes. PROSPERO (CRD42020173119).

Results

After screening 9,108 records, 64 studies were identified as eligible. The pooled prevalence was 19.98% [95% Confidence Intervals [CI]: 16.05–24.23%] for hazardous alcohol use (K = 52), 3.17% [95% CI: 0.95–6.58%] for harmful drinking (K = 8), 14.59% [95% CI: 7.16–25.05%] for dependent drinking (K = 7), and 17.71% [95% CI: 8.34–29.63%] for frequent binge drinking (K = 11). The prevalence of hazardous drinking was greater during the pandemic (28.19%) compared with pre-pandemic estimates (17.95%), though this was not statistically significant (p = 0.049). Studies including all hospital staff (32.04%) showed higher prevalence estimates for hazardous drinking compared with studies of doctors (16.78%) and nurses (27.02%).

Conclusion

Approximately one fifth of healthcare professionals drink to hazardous levels, with higher prevalence estimates observed during the COVID-19 pandemic. It may be that healthcare professionals used alcohol to cope with the additional trauma and stressors. Further research is needed to investigate whether this is sustained in the post-pandemic period.
Assessment of indoor air quality in health clubs: insights into (ultra)fine and coarse particles and gaseous pollutants
Authors: Peixoto Cátia , Pereira Maria do Carmo , Morais Simone , Slezakova Klara
Published: November 2023

Introduction

Exercising on regular basis provides countless health benefits. To ensure the health, well-being and performance of athletes, optimal indoor air quality, regular maintenance and ventilation in sport facilities are essential.

Methods

This study assessed the levels of particulate, down to the ultrafine range (PM10, PM2.5, and particle number concentration in size range of 20–1,000 nm, i.e., – PNC20-1000 nm), gaseous pollutants (total volatile organic compounds – TVOCs, CO2, and O3) and comfort parameters (temperature – T, relative humidity – RH) in different functional spaces of health clubs (n = 8), under specific occupancy and ventilation restrictions.

Results and Discussion

In all HCs human occupancy resulted in elevated particles (up to 2–3 times than those previously reported), considering mass concentrations (PM10: 1.9–988.5 μg/m3 PM2.5: 1.6–479.3 μg/m3) and number (PNC 1.23 × 103 – 9.14 × 104 #/cm3). Coarse and fine PM indicated a common origin (rs = 0.888–0.909), while PNC showed low–moderate associations with particle mass (rs = 0.264–0.629). In addition, up to twice-higher PM and PNC were detected in cardiofitness & bodybuilding (C&B) areas as these spaces were the most frequented, reinforcing the impacts of occupational activities. In all HCs, TVOCs (0.01–39.67 mg/m3) highly exceeded the existent protection thresholds (1.6–8.9 times) due to the frequent use of cleaning products and disinfectants (2–28 times higher than in previous works). On contrary to PM and PNC, TVOCs were higher (1.1–4.2 times) in studios than in C&B areas, due to the limited ventilations combined with the smaller room areas/volumes. The occupancy restrictions also led to reduced CO2 (122–6,914 mg/m3) than previously observed, with the lowest values in HCs with natural airing. Finally, the specific recommendations for RH and T in sport facilities were largely unmet thus emphasizing the need of proper ventilation procedures in these spaces.
The function of mating plugs in the spider Neriene emphana: mating strategy or sperm protection?
Authors: Tian Shuang , Jiang He , Zhan Yongjia , Wu Qingqing , Kuntner Matjaž , Tu Lihong
Published: November 2023

Introduction

It is generally thought that mating plugs, where present, impede or reduce the possibilities of female subsequent mating. Behavioral studies on numerous spiders, where mating plugs are common, have generally supported this function. However, mating plugs in spiders could plausibly serve other functions as well. Namely, the structure of entelegyne spermathecae—the morphology of most spiders—could require a mechanism that would prevent sperm from leakage, desiccation, and backflow. Although the form and function of mating plugs in several spider species imply their potential adaptation for sperm protection, this function has never been empirically tested.

Methods

Here, we test whether mating plugs in the sheet-web spider Neriene emphana serve as a sperm protective device by investigating its genital morphology, its copulation process, and the precise formation of its amorphous mating plugs.

Results

This species constructs secretion plugs through male-female cooperation. Additionally, we found sperm plugs to be formed as a side product of sperm transfer, as well as an intermediate type of secretion plugs. These plug materials are transferred in different mating stages as documented by variations in the rhythm of male palpal application during copulation. We showed that complete copulations always resulted in formation of secretion plugs at spermathecal entrances via laborious deposition of male materials.

Discussion

While our findings do not reject that secretion plugs in N. emphana prevent females from subsequent mating, we suggest that they must have evolved to provide sperm protection.
Unusual localized gingival redness: a case report
Authors: Kumar Swati , Sujir Nanditha , Saha Anwesha , Ahmed Junaid , Bhushan Prem
Published: November 2023

Inflammation of the gingiva is one of the most common and routine findings in dental practice. These routine appearances of inflammatory gingivae can show peculiarity when associated with an underlying systemic condition or because of reactive, benign, or malignant pathologies. This case highlights minute clinical signs of the gingiva that deviate from the routine presentation and warrant further investigations. A 63-year-old woman presented with a chief complaint of severe pain in relation to the lower front teeth region for 1 month. Intraoral examination revealed a gingival lesion on the labial aspect of 41, 42, and 43, and an intraoral periapical radiograph showed mild bone loss. The lesion persisted despite oral prophylaxis, and a biopsy was advised. The final diagnosis was stage 1 gingival squamous cell carcinoma (GSCC). It is important to note that the non-descript presentation of GSCC in early stages often mimics benign traumatic or inflammatory lesions of the gingiva. Peculiar clinical features of GSCC of note include the lack of traditionally associated risk factors and localized red or ulcerative lesions with increased bleeding tendencies that do not respond to routine periodontal treatment within 2 weeks.
The impact of COVID-19 pandemic on pet behavior and human-animal interaction: a longitudinal survey-based study in the United States
Authors: Weng Hsin-Yi , Ogata Niwako
Published: November 2023

Introduction

Although multiple studies have explored behavioral changes in pets during the strict lockdown of the COVID-19 pandemic between May and July 2020, this longitudinal study uniquely investigated the phases beyond strict lockdown. The primary objective of this research was to evaluate the pandemic’s impact on pet behavior.

Methods

To achieve this goal, we conducted an online survey, completed by pet owners residing in the United States between June 2020 (including retrospective data for February and April 2020) and December 2021. The study encompassed four distinct pandemic phases: Pre-pandemic (data collected retrospectively), Strict lockdown, Prolonged lockdown, and Re-opening.

Results and discussion

The data collected from surveys completed by 3,278 pet owners across one to six time points revealed declining trends in all investigated behavior problems of both dogs and cats. Concurrently, human-animal interaction activities also showed variations across different COVID-19 phases. The association between human-animal interactions and the occurrence of behavior problems underscored the importance of not only exercise quantity but also adherence to consistent schedules, particularly for dogs, in mitigating behavior problems. Interestingly, among cats, sleeping outside the bedroom was associated with a reduced occurrence of aggression, anxiety, fear, and physiological behavior changes. However, sleep location displayed limited or no association with behavior problems in dogs. In summary, this study highlights the importance of tailoring interventions to the unique needs of each species through human-animal interactions to mitigate the occurrence of behavioral problems and enhance human-animal relationships.
The state of coursework on horses in human services at universities and colleges in the United States: a scoping review
Authors: Connolly Katherine , Ekholm Fry Nina
Published: November 2023

An increasing number of universities and colleges in the United States are offering coursework on adaptive/therapeutic riding and the incorporation of horses in human service areas such as psychotherapy, education, occupational therapy, physical therapy, and speech-language pathology. The first study to identify coursework in these areas was published in 2018. In order to track development over time, we conducted a replication study to determine the prevalence of coursework on horses in human services at higher education institutions. Information gathered for the 2021–2022 academic year included the institution name, geographic location, number of courses and their focus, academic department offering the course, and level of study. We identified 122 courses provided by 48 higher education institutions in 29 states in the following areas: adaptive/therapeutic riding (N = 82, 67.2%), mental health (N = 19, 15.6%), education/learning (N = 2, 1.6%), and equine movement in physical therapy, occupational therapy, and speech-language pathology (hippotherapy) (N = 1, 0.8%). Survey or overview courses (N = 18, 14.8%) were also identified. These courses were offered both at the undergraduate (N = 114, 93.4%) and the graduate level (N = 8, 6.6%) by a total of 48 departments that either focused on animals, such as equine science, animal science, and agriculture (N = 27, 54%) or focused on humans, such as health science or liberal arts (N = 23, 46%). The results inform a discussion on changes over time as well as current challenges and opportunities for academic programs offering coursework about horses in human services.
Case report: Squamous cell carcinoma of the prostate-a clinicopathological and genomic sequencing-based investigation
Authors: Zhang Caixin , Jia Yong , Kong Qingnuan
Published: November 2023

Squamous differentiation of prostate cancer, which accounts for less than 1% of all cases, is typically associated with androgen deprivation treatment (ADT) or radiotherapy. This entity is aggressive and exhibits poor prognosis due to limited response to traditional treatment. However, the underlying molecular mechanisms and etiology are not fully understood. Previous findings suggest that squamous cell differentiation may potentially arise from prostate adenocarcinoma (AC), but further validation is required to confirm this hypothesis. This paper presents a case of advanced prostate cancer with a combined histologic pattern, including keratinizing SCC and AC. The study utilized whole-exome sequencing (WES) data to analyze both subtypes and identified a significant overlap in driver gene mutations between them. This suggests that the two components shared a common origin of clones. These findings emphasize the importance of personalized clinical management for prostate SCC, and specific molecular findings can help optimize treatment strategies.
Concentration of Heavy Metals in Pollen and Bees Osmia bicornis L. in Three Different Habitats in the Łowicz District in Central Poland
Authors: Zajdel Barbara , Migdał Paweł , Murawska Agnieszka , Jojczyk Agata , Berbeć Ewelina , Kucharska Kornelia , Gąbka Jakub
Published: November 2023

The aim of our research was to compare the levels of pollution in different habitats based on the concentrations of heavy metals found in pollen and the organisms of Osmia bicornis L. bees in three habitats: orchards, berry plantations, and urban habitats (near by power plant, landfill, residential areas, and heavy vehicle traffic). The concentration of Ag, Cd, Cu, Fe, Mn, Ni, Pb and Zn were determined. Samples were quantified using flame atomic absorption spectrophotometry (AAS), with evaluations being carried out three times for each sample. Bee nests were also analyzed concerning reproduction and the presence of parasites. There were no significant differences in the content of heavy metals Ag, Cd, Cu, Fe, Mn, Ni, Pb and Zn in pollen samples between the three habitat types. Bee samples differed only in their Zn content, which was significantly higher in orchards (2.67 mg/kg) than urban habitats (0.80 mg/kg) and berry plantation (0.94 mg/kg). Habitat type had no effect on most bee reproductive parameters (percentage of occupied nest tubes, population growth). Our results show that bees pollinating crops in, for example orchards or berry plantations are exposed to heavy metals to a similar extent to those urban areas.
Design of an Electronically Controlled Fertilization System for an Air-Assisted Side-Deep Fertilization Machine
Authors: Zhu Qingzhen , Zhu Zhihao , Zhang Hengyuan , Gao Yuanyuan , Chen Liping
Published: November 2023

The traditional air-assisted side-deep fertilization device has some problems, such as inaccurate control system parameters and poor precision in variable fertilization. It seriously affects the application and popularization of the device. Aiming at the above problems, this paper wanted to realize the precise fertilizer discharge control of an air-assisted side-deep fertilization device. This paper designs an electronically controlled fertilization system based on a PID controller from the past. The system model was constructed in MATLAB, and the mathematical model and transfer function model of a stepper motor, the mathematical model of fertilizer discharge, and the stepper motor rotational speed were established too. In order to improve the accuracy of precise fertilizer discharge control system parameters, the system parameters were optimized based on the particle swarm optimization algorithm and the control system tuner toolbox. We had established a validation test platform to test the performance of a precise fertilizer discharge control system. In the actual experiment, the maximum stability coefficient of variation was 0.91% at the target fertilizer discharge mass level of 350 g/min, and the maximum error of fertilizer discharge was 4.14% at 550 g/min of the target fertilizer discharge mass level. By analyzing the test results of the precise fertilizer discharge control system, the new precise fertilizer discharge control system had good fertilizer discharge stability and could also meet the technical specification for quality evaluation of fertilization machinery (NY/T 1003-2006). This research can improve the fertilizer discharge accuracy of the air-assisted side-deep fertilization control system.
Postharvest Biological Control of Guatemalan Potato Moth, Tecia solanivora, by Trichogramma euproctidis and Blattisocius tarsalis
Authors: Gavara Jorge , Paris Mihaela , Hernández-Suárez Estrella , Cabello Tomás , Gallego Juan Ramón , Piedra-Buena Ana
Published: November 2023

Tecia solanivora (Povolný 1973), is a quarantine pest in Europe. Identified in Guatemala in the 1970s, it spread throughout Central and South America, reaching the Canary Islands in 1999 and mainland Spain in 2015. The pest has caused prohibitive economic losses both in the field and in storage, where losses can reach 100%. In the absence of approved chemical treatments, the use of an egg predatory mite, Blattisocius tarsalis (Berlese 1918), and the egg parasitoid, Trichogramma euproctidis (Girault 1911), is being studied for use in storage. Previous laboratory studies have confirmed their potential for use in non-refrigerated stores, typically between 15 and 20 °C. In the present work, we compared the efficacy of both natural enemies under semi-storage conditions. We observed that while both T. euproctidis and B. tarsalis reduced the T. solanivora populations (with an efficacy of 82.95 ± 7.32% and 49.06 ± 2.69%, respectively) and the number of mites per tuber, only B. tarsalis resulted in undamaged tubers (65%). For this reason, the mite was selected and tested in storage conditions, obtaining promising results in the protection of infested tubers, suggesting potential for further investigation, adaptation and standardization of its use in real conditions.
Inflammatory Status and Chronic Kidney Disease in Cats: Old and New Inflammatory Markers—A Pilot Prospective Study
Authors: Uva Annamaria , Cavalera Maria Alfonsa , Gusatoaia Oana , Donghia Rossella , Gernone Floriana , Silvestrino Marco , Zatelli Andrea
Published: November 2023

This prospective study aimed to evaluate inflammatory status in cats affected by chronic kidney disease (CKD) at IRIS stages 2–4, using serum amyloid A (SAA) and the erythrocyte sedimentation rate (ESR) as inflammatory markers. Thirty-two cats with CKD and ten clinically healthy cats (i.e., control group) were enrolled. The recording of signalment data, complete physical examinations, and abdominal ultrasonography were performed for each animal. Additionally, ESR levels, complete blood count, clinical chemistry (including SAA determination), serum protein electrophoresis, and complete urinalysis were executed. This study’s results showed that mean ESR and SAA concentrations in cats with CKD were statistically higher compared to those of the control group (p = 0.0005 and p = 0.007, respectively). The SAA concentration was significantly increased at IRIS stages 2, 3, and 4 compared to the control group. Meanwhile, the ESR was significantly higher in cats at IRIS stages 3 and 4 (p = 0.0003 and p = 0.0007, respectively), but not at IRIS stage 2, compared to the control group. These results provide evidence that feline CKD is associated with a systemic inflammatory status. Moreover, the rise in ESR appears to be more linked to advanced stages of the disease and could, therefore, correlate with the uremic condition.
Nutrient and Rumen Fermentation Studies of Indian Pasture Legumes for Sustainable Animal Feed Utilisation in Semiarid Areas
Authors: Singh Sultan , Singh Tejveer , Koli Pushpendra , Anele Uchenna Y. , Bhadoria Brijesh K. , Choudhary Mukesh , Ren Yonglin
Published: November 2023

This study evaluated 5 annual and 11 perennial Indian pasture legumes species for their nutritive value, dry matter and mineral contents and in vitro fermentation parameters. Legume species differed significantly (p < 0.05) in various nutritional aspects such as organic matter, crude protein (CP), ether extract, fibres and protein fractions. Perennial Clitoria ternateaa had higher (p < 0.05) buffer soluble protein (477), while neutral detergent soluble protein was highest in annually grown Lablab purpureus (420 g/kg CP). Atylosia scarabaeoides (AS) had higher levels of nonstructural carbohydrates (NSCs) (392 g/kg dry matter (DM)) than structural carbohydrates (SC) (367 g/kg DM). Its rapidly degradable fraction (51.7 g/kg (total carbohydrate) tCHO) was lower (p < 0.05) than other fractions of carbohydrates. Total digestible nutrients, digestible energy and metabolisable energy varied, with Desmodium virgatus (DV) having higher values and Stylosanthas seabrana (SSe) having the lowest. Predicted dry matter intake, digestible dry matter and relative feed value also showed significant differences (p < 0.05). Annual grasses such as Dolichos biflorus, Macroptilium atropurpureum, Rhynchosia minima (RM) were found to be better balanced with micro minerals. In vitro dry matter degradability, partition factor, short-chain fatty acids and microbial protein production of legumes varied significantly (p < 0.05). Gas and CH4 production (mL/g and mL/g (digestible DM) DDM) also varied, with Clitoria ternatea-blue having the highest gas production and C. ternatea -white (CT-w) and AS having lower CH4 production. Methane in total gas was low for DV, RM and CT-w (8.99%, 9.72% and 9.51%). Loss of DE and ME as CH4 varied (p < 0.05) among the legumes. Each legume offers unique benefits, potentially allowing for tailored combinations of annual and perennial legumes to optimize rumen feed efficiency.
Genetically Related Avian Influenza H7N9 Viruses Exhibit Different Pathogenicity in Mice
Authors: Wang Xiaoquan , Zheng Huafen , Gao Ruyi , Ren Leyao , Jin Mingxia , Ji Zhuxing , Wang Xin , Lu Xiaolong , Yang Wenhao , Gu Min , Liu Xiaowen , Hu Shunlin , Liu Kaituo , Liu Xiufan
Published: November 2023

Avian influenza viruses can cross species barriers and adapt to mammals. The H7N9 subtype AIV that emerged in China in 2013 caused 1568 human infections, with a mortality rate of nearly 40%. We conducted a retrospective analysis of H7N9 viruses that were isolated in live poultry markets in 2013. We found that two avian-origin H7N9 isolates, A/chicken/Eastern China/JTC4/2013 and A/chicken/Eastern China/JTC11/2013, have a similar genetic background but exhibit different pathogenicity in mice. Whole-genome alignment of the two H7N9 viruses was carried out, and only six amino acid differences mapped in five genes, including the well-known virulence molecular marker PB2-E627K. Our retrospective analysis highlighted the importance of monitoring the adaptive mutations in avian influenza viruses with zoonotic potential.
Leveraging Accelerometer Data for Lameness Detection in Dairy Cows: A Longitudinal Study of Six Farms in Germany
Authors: Lavrova Anastasia I. , Choucair Alexander , Palmini Andrea , Stock Kathrin F. , Kammer Martin , Querengässer Friederike , Doherr Marcus G. , Müller Kerstin E. , Belik Vitaly
Published: November 2023

Lameness in dairy cows poses a significant challenge to improving animal well-being and optimizing economic efficiency in the dairy industry. To address this, employing automated animal surveillance for early lameness detection and prevention through activity sensors proves to be a promising strategy. In this study, we analyzed activity (accelerometer) data and additional cow-individual and farm-related data from a longitudinal study involving 4860 Holstein dairy cows on six farms in Germany during 2015–2016. We designed and investigated various statistical models and chose a logistic regression model with mixed effects capable of detecting lameness with a sensitivity of 77%. Our results demonstrate the potential of automated animal surveillance and hold the promise of significantly improving lameness detection approaches in dairy livestock.
Management and Prognosis of Acute Post-Cataract Surgery Endophthalmitis: A 10-Year Retrospective Analysis in Eastern China
Authors: Zhang Xiuwen , Chen Zhi , Li Xiaoxia , Zhou Zimei , Boost Maureen , Huang Taomin , Zhou Xingtao
Published: November 2023

Acute post-cataract surgery endophthalmitis (APSE) is a serious vision-threatening complication of cataract surgery. Analysis of the management and prognosis in cases of APSE may provide better guidance for future treatment. Fifty-six patients (56 eyes) diagnosed with APSE between 2013 and 2022 were retrospectively reviewed. The incidence of APSE rate was 0.020% (95% CI: 0.011–0.029%). Intraocular cultures were positive in 18 (32.1%) cases, with 21 organisms isolated. Coagulase-negative staphylococci was the predominant isolate (12/21; 57.1%). The time from surgery to the onset of endophthalmitis was 7 days (interquartile range: 3–16) in patients with good best-corrected visual acuity (BCVA) (≥20/70) and 3 days (interquartile range: 1–8) in those with poor BCVA (<20/70). Multivariate linear regression analysis revealed that initial BCVA (logMAR) (p < 0.001), time from onset to initial intravitreal antibiotics (IVAs) (p < 0.001), and positive culture of highly virulent pathogens (p = 0.018) displayed significantly positive associations with the final BCVA (logMAR). Adjunctive use of intravitreal corticosteroids and systemic antibiotics were unrelated to a favorable final BCVA. In conclusion, the severity of the visual condition at baseline, as well as delayed treatment, are risk factors for poor visual outcomes in APSE.
The Effectiveness of Pelvic Floor Muscle Exercise with Biofeedback in Women with Urinary Incontinence: A Systematic Review
Authors: Matsi Aikaterini Evangelia , Billis Evdokia , Lampropoulou Sofia , Xergia Sofia A. , Tsekoura Maria , Fousekis Konstantinos
Published: November 2023

Urinary incontinence affects approximately 200 million people worldwide. The objective of this study was to investigate the effect of pelvic floor muscle (PFM) training with biofeedback (BF) in women with urinary incontinence in comparison to PFM training alone. The primary outcome was PFM strength with secondary outcomes being the severity of incontinence, other PFM parameters, quality of life (QoL), social life, satisfaction and adherence to treatment. Randomized controlled trials (RCTs) published from 2005 to 2023 in PubMed, MEDLINE, Scopus and Google Scholar were searched and evaluated with the PEDro scale. Nine moderate and two high methodological quality RCTs were selected. There was a statistically significant improvement in all parameters. In five studies, muscle strength and the severity of incontinence were statistically better in the BF group compared to the non-BF group. In most studies, no differences were found for QoL and social life between the groups. For adherence to treatment, the results were ambiguous. Τhe remaining variables (endurance, precontraction, function, adherence and satisfaction) had few studies to support the results. There was moderate level evidence that the group utilizing PFM exercises with BF showed significant improvements in the management of urinary incontinence. Concerning muscle strength and the severity of incontinence, findings were inconclusive, as only in some studies the results were statistically better in the BF group compared to the non-BF group.