bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Proteomic Analysis of Developmental Changes in P-Type Cytoplasmic Male Sterile and Maintainer Anthers in Wheat
Authors: Zhang Yamin , Song Qilu , Zhang Lili , Li Zheng , Wang Chengshe , Zhang Gaisheng
Published: February 2021

Cytoplasmic male sterility (CMS) plays an important role in the application of heterosis in wheat (Triticum aestivum L.). However, the molecular mechanism underlying CMS remains unknown. This study provides a comprehensive morphological and proteomic analysis of the anthers of a P-type CMS wheat line (P) and its maintainer line, Yanshi 9 hao (Y). Cytological observations indicated that the P-type CMS line shows binucleate microspore abortion. In this line, the tapetum degraded early, leading to anther cuticle defects, which could not provide the nutrition needed for microspore development in a timely manner, thus preventing the development of the microspore to the normal binucleate stage. Proteomic analysis revealed novel proteins involved in P-type CMS. Up to 2576 differentially expressed proteins (DEPs) were quantified in all anthers, and these proteins were significantly enriched in oxidative phosphorylation, glycolysis/gluconeogenesis, citrate cycle (TCA cycle), starch and sucrose metabolism, phenylpropanoid biosynthesis, and pyruvate metabolism pathways. These proteins may comprise a network that regulates male sterility in wheat. Based on the function analysis of DEPs involved in the complex network, we concluded that the P-type CMS line may be due to cellular dysfunction caused by disturbed carbohydrate metabolism, inadequate energy supply, and disturbed protein synthesis. These results provide insights into the molecular mechanism underlying male sterility and serve as a valuable resource for researchers in plant biology, in general, and plant sexual reproduction, in particular.
Exosomal Cargo May Hold the Key to Improving Reproductive Outcomes in Dairy Cows
Authors: Turner Natalie , Abeysinghe Pevindu , Sadowski Pawel , Mitchell Murray D.
Published: February 2021

The reproductive status of dairy cows remains a challenge for dairy farmers worldwide, with impaired fertility linked to a significant reduction in herd profitability, due in part to impaired immunity, increased metabolic pressure, and longer postpartum anestrous interval (PPAI). Exosomes are nanovesicles released from a variety of cell types and end up in circulation, and carry proteins, bioactive peptides, lipids, and nucleic acids specific to the place of origin. As such, their role in health and disease has been investigated in humans and animals. This review discusses research into exosomes in the context of reproduction in dairy herds and introduces recent advances in mass-spectrometry (MS) based proteomics that have a potential to advance quantitative profiling of exosomal protein cargo in a search for early biomarkers of cattle fertility.
Genome-Wide Characterization, Evolution, and Expression Profile Analysis of GATA Transcription Factors in Brachypodium distachyon
Authors: Peng Weiye , Li Wei , Song Na , Tang Zejun , Liu Jing , Wang Yunsheng , Pan Sujun , Dai Liangying , Wang Bing
Published: February 2021

The GATA proteins, functioning as transcription factors (TFs), are involved in multiple plant physiological and biochemical processes. In this study, 28 GATA TFs of Brachypodium distachyon (BdGATA) were systematically characterized via whole-genome analysis. BdGATA genes unevenly distribute on five chromosomes of B. distachyon and undergo purifying selection during the evolution process. The putative cis-acting regulatory elements and gene interaction network of BdGATA were found to be associated with hormones and defense responses. Noticeably, the expression profiles measured by quantitative real-time PCR indicated that BdGATA genes were sensitive to methyl jasmonate (MeJA) and salicylic acid (SA) treatment, and 10 of them responded to invasion of the fungal pathogen Magnaporthe oryzae, which causes rice blast disease. Genome-wide characterization, evolution, and expression profile analysis of BdGATA genes can open new avenues for uncovering the functions of the GATA genes family in plants and further improve the knowledge of cellular signaling in plant defense.
Increased Histone Acetylation and Decreased Expression of Specific Histone Deacetylases in Ultraviolet-Irradiated and Intrinsically Aged Human Skin In Vivo
Authors: Lee Yuri , Shin Mi Hee , Kim Min-Kyoung , Kim Yeon Kyung , Shin Hye Sun , Lee Dong Hun , Chung Jin Ho
Published: February 2021

Histone deacetylases (HDACs) are conserved enzymes that remove acetyl groups from lysine side chains in histones and other proteins and play a crucial role in epigenetic regulation. Previously, we showed that histone acetylation is implicated in ultraviolet (UV)-induced inflammation and matrix impairment. To elucidate the histone acetylation status and specific HDACs involved in skin aging, we examined the changes in histone acetylation, global HDAC activity, and the expression of HDACs and sirtuins (SIRTs) in intrinsically aged and photoaged human skin as well as in UV-irradiated human skin in vivo. Following acute UV irradiation, the acetylated histone H3 (AcH3) level was increased, but HDAC activity and the expression levels of HDAC4, HDAC11, and SIRT4 were significantly decreased. In intrinsically aged skin, AcH3 levels were increased, but HDAC activity and the expression levels of HDAC4, HDAC5, HDAC10, HDAC11, SIRT6, and SIRT7 were significantly decreased. However, histone acetylation and HDAC expression in photoaged skin were not significantly different from those in intrinsically aged skin. Collectively, HDAC4 and HDAC11 were decreased in both UV-irradiated and intrinsically aged skin, suggesting that they may play a universal role in increased histone acetylation associated with skin aging.
Human Costars Family Protein ABRACL Modulates Actin Dynamics and Cell Migration and Associates with Tumorigenic Growth
Authors: Hsiao Bo-Yuan , Chen Chia-Hsin , Chi Ho-Yi , Yen Pei-Ru , Yu Ying-Zhen , Lin Chia-Hsin , Pang Te-Ling , Lin Wei-Chi , Li Min-Lun , Yeh Yi-Chen , Chou Teh-Ying , Chen Mei-Yu
Published: February 2021

Regulation of cellular actin dynamics is pivotal in driving cell motility. During cancer development, cells migrate to invade and spread; therefore, dysregulation of actin regulators is often associated with cancer progression. Here we report the role of ABRACL, a human homolog of the Dictyostelium actin regulator Costars, in migration and tumorigenic growth of cancer cells. We found a correlation between ABRACL expression and the migratory ability of cancer cells. Cell staining revealed the colocalization of ABRACL and F-actin signals at the leading edge of migrating cells. Analysis of the relative F-/G-actin contents in cells lacking or overexpressing ABRACL suggested that ABRACL promotes cellular actin distribution to the polymerized fraction. Physical interaction between ABRACL and cofilin was supported by immunofluorescence staining and proximity ligation. Additionally, ABRACL hindered cofilin-simulated pyrene F-actin fluorescence decay in vitro, indicating a functional interplay. Lastly, analysis on a colorectal cancer cohort demonstrated that high ABRACL expression was associated with distant metastasis, and further exploration showed that depletion of ABRACL expression in colon cancer cells resulted in reduced cell proliferation and tumorigenic growth. Together, results suggest that ABRACL modulates actin dynamics through its interaction with cofilin and thereby regulates cancer cell migration and participates in cancer pathogenesis.
Copper Dependent Modulation of α-Synuclein Phosphorylation in Differentiated SHSY5Y Neuroblastoma Cells
Authors: Greco Marco , Spinelli Chiara Carmela , De Riccardis Lidia , Buccolieri Alessandro , Di Giulio Simona , Musarò Debora , Pagano Claudia , Manno Daniela , Maffia Michele
Published: February 2021

Copper (Cu) dyshomeostasis plays a pivotal role in several neuropathologies, such as Parkinson’s disease (PD). Metal accumulation in the central nervous system (CNS) could result in loss-of-function of proteins involved in Cu metabolism and redox cycling, generating reactive oxygen species (ROS). Moreover, neurodegenerative disorders imply the presence of an excess of misfolded proteins known to lead to neuronal damage. In PD, Cu accumulates in the brain, binds α-synuclein, and initiates its aggregation. We assessed the correlation between neuronal differentiation, Cu homeostasis regulation, and α-synuclein phosphorylation. At this purpose, we used differentiated SHSY5Y neuroblastoma cells to reproduce some of the characteristics of the dopaminergic neurons. Here, we reported that differentiated cells expressed a significantly higher amount of a copper transporter protein 1 (CTR1), increasing the copper uptake. Cells also showed a significantly more phosphorylated form of α-synuclein, further increased by copper treatment, without modifications in α-synuclein levels. This effect depended on the upregulation of the polo-like kinase 2 (PLK2), whereas the levels of the relative protein phosphatase 2A (PP2A) remained unvaried. No changes in the oxidative state of the cells were identified. The Cu dependent alteration of α-synuclein phosphorylation pattern might potentially offer new opportunities for clinical intervention.
Immune-Mediated Aggravation of the Campylobacter concisus-Induced Epithelial Barrier Dysfunction
Authors: Nattramilarasu Praveen Kumar , Lobo de Sá Fábia Daniela , Schulzke Jörg-Dieter , Bücker Roland
Published: February 2021

Campylobacter concisus is a human-pathogenic bacterium of the gastrointestinal tract. This study aimed at the contribution of the mucosal immune system in the context of intestinal epithelial barrier dysfunction induced by C. concisus. As an experimental leaky gut model, we used in vitro co-cultures of colonic epithelial cell monolayers (HT-29/B6-GR/MR) with M1-macrophage-like THP-1 cells on the basal side. Forty-eight hours after C. concisus infection, the decrease in the transepithelial electrical resistance in cell monolayers was more pronounced in co-culture condition and 22 ± 2% (p < 0.001) higher than the monoculture condition without THP-1 cells. Concomitantly, we observed a reduction in the expression of the tight junction proteins occludin and tricellulin. We also detected a profound increase in 4 kDa FITC-dextran permeability in C. concisus-infected cell monolayers only in co-culture conditions. This is explained by loss of tricellulin from tricellular tight junctions (tTJs) after C. concisus infection. As an underlying mechanism, we observed an inflammatory response after C. concisus infection through pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) released from THP-1 cells in the co-culture condition. In conclusion, the activation of subepithelial immune cells exacerbates colonic epithelial barrier dysfunction by C. concisus through tricellulin disruption in tTJs, leading to increased antigen permeability (leaky gut concept).
Exosomes Secreted from Amniotic Membrane Contribute to Its Anti-Fibrotic Activity
Authors: Mao Yong , Jacob Vimal , Singal Amit , Lei Shunyao , Park Min Sung , Lima Mariana R.N. , Li Chaoyang , Dhall Sandeep , Sathyamoorthy Malathi , Kohn Joachim
Published: February 2021

Amniotic membranes (AM) have anti-fibrotic activity. Exosomes (nano-sized vesicles) function as conduits for intercellular transfer and contain all the necessary components to induce the resolution of fibrosis. In this study, we tested the hypothesis that the anti-fibrotic activity of AM is mediated by exosomes. AM-derived exosomes or amniotic stromal cell-derived exosomes were isolated and characterized. Anti-fibrotic activity of exosomes was evaluated using human hepatic stellate cells (LX-2), an in vitro model of fibrosis. Exosomes isolated from AM tissue-conditioned media had an average size of 75 nm. Exosomes significantly inhibited the proliferation of TGFβ1-activated LX-2 but had no effect on the proliferation of non-activated LX-2 cells. Exosomes also reduced the migration of LX-2 in a scratch wound assay. Furthermore, exosomes reduced the gene expression of pro-fibrotic markers such as COL1A1, ACTA, and TGFβ1 in LX-2 cells. Interestingly, exosomes isolated from AM tissue under hypoxic conditions seemed to show a stronger anti-fibrotic activity than exosomes isolated from tissue under normoxic conditions. Exosomes released by in vitro cultured AM stromal cells were smaller in size compared with tissue exosomes and also showed anti-fibrotic activity on LX-2 cells. In conclusion, AM-tissue-released exosomes contribute to the anti-fibrotic activity of AM. This is the first report of isolation, characterization, and functional evaluation of exosomes derived from amniotic tissues with the direct comparison between tissue-derived exosomes and cultured cell-derived exosomes.
Long-Read Sequencing Improves the Detection of Structural Variations Impacting Complex Non-Coding Elements of the Genome
Authors: Begum Ghausia , Albanna Ammar , Bankapur Asma , Nassir Nasna , Tambi Richa , Berdiev Bakhrom K. , Akter Hosneara , Karuvantevida Noushad , Kellam Barbara , Alhashmi Deena , Sung Wilson W. L. , Thiruvahindrapuram Bhooma , Alsheikh-Ali Alawi , Scherer Stephen W. , Uddin Mohammed
Published: February 2021

The advent of long-read sequencing offers a new assessment method of detecting genomic structural variation (SV) in numerous rare genetic diseases. For autism spectrum disorders (ASD) cases where pathogenic variants fail to be found in the protein-coding genic regions along chromosomes, we proposed a scalable workflow to characterize the risk factor of SVs impacting non-coding elements of the genome. We applied whole-genome sequencing on an Emirati family having three children with ASD using long and short-read sequencing technology. A series of analytical pipelines were established to identify a set of SVs with high sensitivity and specificity. At 15-fold coverage, we observed that long-read sequencing technology (987 variants) detected a significantly higher number of SVs when compared to variants detected using short-read technology (509 variants) (p-value < 1.1020 × 10−57). Further comparison showed 97.9% of long-read sequencing variants were spanning within the 1–100 kb size range (p-value < 9.080 × 10−67) and impacting over 5000 genes. Moreover, long-read variants detected 604 non-coding RNAs (p-value < 9.02 × 10−9), comprising 58% microRNA, 31.9% lncRNA, and 9.1% snoRNA. Even at low coverage, long-read sequencing has shown to be a reliable technology in detecting SVs impacting complex elements of the genome.
The Lymphatic System in Zebrafish Heart Development, Regeneration and Disease Modeling
Authors: Feng Xidi , Travisano Stanislao , Pearson Caroline A. , Lien Ching-Ling , Harrison Michael R. M.
Published: February 2021

Heart disease remains the single largest cause of death in developed countries, and novel therapeutic interventions are desperately needed to alleviate this growing burden. The cardiac lymphatic system is the long-overlooked counterpart of the coronary blood vasculature, but its important roles in homeostasis and disease are becoming increasingly apparent. Recently, the cardiac lymphatic vasculature in zebrafish has been described and its role in supporting the potent regenerative response of zebrafish heart tissue investigated. In this review, we discuss these findings in the wider context of lymphatic development, evolution and the promise of this system to open new therapeutic avenues to treat myocardial infarction and other cardiopathologies.
The Efficacy and Safety of Dual-Frequency Ultrasound for Improving Skin Hydration and Erythema in Patients with Rosacea and Acne
Authors: Kim Young Jae , Moon Ik Jun , Lee Hae Woong , Won Chong Hyun , Chang Sung Eun , Lee Mi Woo , Choi Jee Ho , Lee Woo Jin
Published: February 2021

Inflammatory skin diseases, such as rosacea and acne, are major causes of facial erythema and accompanying skin barrier dysfunction. Several methods to restore the impaired skin barrier and improve facial erythema, such as medication, radiofrequency, laser, and ultrasound therapy were attempted. This study evaluated the efficacy and safety of dual-frequency ultrasound with impulse mode, for improving skin hydration and erythema in Asian subjects with rosacea and acne. Twenty-six subjects with facial erythema received an ultrasound treatment once per week, for 4 weeks, over both cheeks. The erythema index and transepidermal water loss (TEWL) were measured at each visit. Clinicians assessed the erythema improvement and patients evaluated their satisfaction level. The average decrease in TEWL and erythema index at 6 weeks was 5.37 ± 13.22 g·h−1·m−2 (p = 0.020) and 39.73 ± 44.21 (p = 0.010), respectively. The clinician’s erythema assessment and the subject satisfaction questionnaire score significantly improved at final follow-up (p < 0.001; p = 0.003, respectively). No serious adverse effects were observed during the treatment and follow-up periods. The dual-frequency ultrasound with impulse mode appears to be effective and safe for improving skin hydration and erythema in patients with rosacea and acne.
Immunological Characteristics of Non-Intensive Care Hospitalized COVID-19 Patients: A Preliminary Report
Authors: Corrao Salvatore , Gervasi Francesco , Di Bernardo Francesca , Natoli Giuseppe , Raspanti Massimo , Catalano Nicola , Argano Christiano
Published: February 2021

The outbreak of coronavirus disease 2019 (COVID-19) is posing a threat to global health. This disease has different clinical manifestations and different outcomes. The immune response to the novel 2019 coronavirus is complex and involves both innate and adaptive immunity. In this context, cell-mediated immunity plays a vital role in effective immunity against SARS-CoV-2. Significant differences have been observed when comparing severe and non-severe patients. Since these immunological characteristics have not been fully elucidated, we aimed to use cluster analysis to investigate the immune cell patterns in patients with COVID-19 who required hospitalization but not intensive care. We identified four clusters of different immunological patterns, the worst being characterized by total lymphocytes, T helper lymphocytes CD4+ (CD4+), T cytotoxic lymphocytes CD8+ (CD8+) and natural killer (NK) cells below the normal range, together with natural killer lymphocyte granzyme < 50% (NK granzyme+) and antibody-secreting plasma cells (ASCs) equal to 0 with fatal outcomes. In the worst group, 50% of patients died in the intensive care unit. Moreover, a negative trend was found among four groups regarding total lymphocytes, CD4+, CD8+ and B lymphocytes (p < 0.001, p < 0.005, p < 0.000, p < 0.044, respectively). This detailed analysis of immune changes may have prognostic value. It may provide a new perspective for identifying subsets of COVID-19 patients and selecting novel prospective treatment strategies. Notwithstanding these results, this is a preliminary report with a small sample size, and our data may not be generalizable. Further cohort studies with larger samples are necessary to quantify the prognostic value’s weight, according to immunological changes in COVID-19 patients, for predicting prognoses and realizing improvements in clinical conditions.
Validity of Clinical Symptoms Score to Discriminate Patients with COVID-19 from Common Cold Out-Patients in General Practitioner Clinics in Japan
Authors: Sonoda Shiro , Kuramochi Jin , Matsuyama Yusuke , Miyazaki Yasunari , Fujiwara Takeo
Published: February 2021

Objective: Coronavirus disease 2019 (COVID-19) has spread worldwide, including Japan. However, little is known about the clinical symptoms which discriminate between COVID-19 and non-COVID-19 among outpatients in general practitioner clinics, which is important for efficient case detection. The aim of this study was to investigate the clinical symptoms to discriminate between COVID-19 and non-COVID-19 cases among outpatients in general practitioner clinics during the second wave of the COVID-19 pandemic in Japan in August 2020. Methods: The records of 360 patients who visited a clinic with suspicion of infectious disease and underwent COVID-19 PCR test between 1 and 14 August 2020 were used. The patients filled out a questionnaire on possible clinical symptoms and transmission routes. Multivariate logistic regression was used to investigate the association between clinical symptoms and COVID-19 status. Results: COVID-19-positive patients were 17 (4.7%). Multiple logistic regression analyses showed that anosmia (odds ratio (OR), 25.94 95% confidence interval (CI), 7.15–94.14; p < 0.001), headache (OR, 3.31 95% confidence interval (CI), 0.98–11.20; p = 0.054), sputum production (OR, 3.32 CI, 1.01–10.90; p = 0.048) and history of visiting an izakaya or bar (OR, 4.23 CI, 0.99–18.03; p = 0.051) were marginally significantly associated withbeing COVID-19 positive. This model showed moderate predictive power (area under receiver operating characteristic curve = 0.870 CI, 0.761 to 0.971). Conclusions: We found that anosmia, headache, sputum production, history of visiting an izakaya or bar were associated with COVID-19, which can be used to detect patients with COVID-19 in out-patient clinics in Japan. The findings of this study need to be verified in other clinics and hospitals in Japan and other countries with universal healthcare coverages.
Oxidative Stress Reduction by Midazolam Premedication during Oocyte Retrieval Procedure: Pilot Study
Authors: Pešić Maja , Kličan-Jaić Katarina , Vučić Marinko , Kuna Krunoslav , Košec Andro , Čipak Gašparović Ana
Published: February 2021

Infertility is one of the major medical problems nowadays. Couples who opt for In Vitro Fertilization (IVF) face a great deal of stress which certainly affects the outcome of the procedure. Therefore, we aimed to reduce the stress during the oocyte retrieval procedure by applying midazolam. Total oxidant (TOC) and antioxidant (TAC) capacities of serum, as well as glutathione (GSH) content and catalase activity, were measured in both control and midazolam groups. Follicular fluid was also tested for oxidant capacity and IL1β. Results implied that the midazolam group increased TAC at the end of the procedure. At the same time, the control group decreased GSH at the beginning of the procedure, and both groups decreased catalase activity at the end of the procedure. The results imply that stress during the procedure affects oxidative and antioxidative parameters of the patients, but did not affect the frequency of the pregnancy at the end of this pilot study. Yet, the results imply that oxidative and antioxidative mechanisms during IVF should be investigated in detail as they could affect the outcome of IVF.
Eccentric Resistance Training in Youth: A Survey of Perceptions and Current Practices by Strength and Conditioning Coaches
Authors: Drury Benjamin , Clarke Hannah , Moran Jason , Fernandes John F. T. , Henry Greg , Behm David G.
Published: February 2021

Background: Eccentric resistance training (ERT) in youth is advocated for aiding performance and injury risk. However, research investigating the applied practices of ERT in youth is in its infancy. In this study, we surveyed the perceptions and practices of practitioners utilizing ERT in youth to provide an understanding of its current application in practice. Methods: Sixty-four strength and conditioning coaches completed an online survey reporting their current use of ERT in youth using both open and closed questions. Results: Coaches deemed the inclusion of ERT important in youth with its inclusion based upon factors such as maturation status, training age and strength levels. Coaches also displayed an awareness of the physiological responses to eccentric exercise in youth compared to adults. ERT was primarily used for injury prevention, with the majority of coaches using body-weight and tempo exercises. Furthermore, utilizing eccentric hamstrings exercises was reported as highly important. The frequency of ERT tended to increase in older age groups and coaches mainly prescribed self-selected rest intervals. Finally, the need for further research into the training guidelines of ERT in youth was highlighted, in which coaches require more information on how maturation influences training adaptations and the fatigue–recovery responses. Conclusion: Coaches emphasized the importance of including ERT for both performance and injury prevention factors in youth although further research is required to generate practical guidelines for coaches in order to support its inclusion within practice.
Reanalysis of the Sydney Harbor RiverCat Ferry
Authors: Doctors Lawrence J.
Published: February 2021

In this paper, we revisit the hydrodynamics supporting the design and development of the RiverCat class of catamaran ferries operating in Sydney Harbor since 1991. More advanced software is used here. This software accounts for the hydrodynamics of the transom demisterns that experience partial or full ventilation, depending on the vessel speed. This ventilation gives rise to the hydrostatic drag, which adds to the total drag of the vessel. The presence of the transom also creates a hollow in the water. This hollow causes an effective hydrodynamic lengthening of the vessel, which leads to a reduction in the wave resistance. Hence, a detailed analysis is required in order to optimize the size of the transom. It is demonstrated that the drag of the vessel and the wave generation can be predicted with good accuracy. Finally, the software is also used to optimize the vessel further by means of affine transformations of the hull geometry.
Altimeter Observations of Tropical Cyclone-generated Sea States: Spatial Analysis and Operational Hindcast Evaluation
Authors: Collins Clarence , Hesser Tyler , Rogowski Peter , Merrifield Sophia
Published: February 2021

Tropical cyclones (TC) are some of the most intense weather systems on Earth and are responsible for generating hazardous waves on the sea surface that dominate the extreme wave climate in several regions, including the Gulf of Mexico and the U.S. East Coast. Modeling these waves is crucial for engineering applications, yet it is notoriously difficult, due to TC’s compact structure and rapid evolution in space and time relative to other weather systems. To better understand the wave structure under TCs, we use satellite altimeter data paired with TC tracks. We parse the data by TC intensity and forward translation velocity, finding evidence of extended fetch. We use the altimeter data to evaluate operational hindcasts, including the US Army Corps of Engineer’s Wave Information Study, National Oceanic and Atmospheric Administration’s National Centers for Environmental Prediction Production Hindcast, and the Institut français de recherche pour l’exploitation de la mer (Ifremer) hindcast. The Ifremer hindcast (1990–2016) is examined in detail. Near the eye in the TC-centered reference frame, we find a pattern of model underestimation in the left sector and overestimation in the right sector except near the eye where wave height remains underestimated. This pattern holds, albeit modulated, across various intensities, forward translation velocities, and radii of maximum winds; the exceptions being the fastest translating storms where the error pattern shows a trend towards overestimation in all sectors. The error patterns for intense and compact TCs exhibited more severe underestimation, which dominated the region near the TC eye.
Dynamic Evacuation Planning on Cruise Ships Based on an Improved Ant Colony System (IACS)
Authors: Liu Linfan , Zhang Huajun , Xie Jupeng , Zhao Qin
Published: February 2021

The emergency evacuation route planning of cruise ships directly affects the safety of all crew members and passengers during emergencies. Research on the planning of emergency evacuation routes for cruise ships is a frontier subject of maritime safety. This study proposes an improved ant colony system (IACS) to solve the evacuation route planning of crowds on cruise ships. The IACS, which is different from common single-path ant colony system (ACS) evacuation algorithms, is used to solve the multipath planning problem of crowd evacuation from cruise ships by considering crowd density and speed in the model. An increasing flow method is introduced into the IACS to improve the efficiency of the proposed algorithm. Numerical experiments show that this method meets the requirements of evacuation analysis guidelines for new and existing passenger ships (MSC.1/Circ.1533)and can effectively and efficiently plan the emergency evacuation path for cruise ship crowd.
Fracture Load of Metal, Zirconia and Polyetheretherketone Posterior CAD-CAM Milled Fixed Partial Denture Frameworks
Authors: Rodríguez Verónica , Tobar Celia , López-Suárez Carlos , Peláez Jesús , Suárez María J.
Published: February 2021

The aim of this study was to investigate the load to fracture and fracture pattern of prosthetic frameworks for tooth-supported fixed partial dentures (FPDs) fabricated with different subtractive computer-aided design and computer-aided manufacturing (CAD-CAM) materials. Materials and Methods: Thirty standardized specimens with two abutments were fabricated to receive three-unit posterior FDP frameworks with an intermediate pontic. Specimens were randomly divided into three groups (n = 10 each) according to the material: group 1 (MM)—milled metal; group 2 (L)—zirconia; and group 3 (P)—Polyetheretherketone (PEEK). The specimens were thermo-cycled and subjected to a three-point bending test until fracture using a universal testing machine (cross-head speed: 1 mm/min). Axial compressive loads were applied at the central fossa of the pontics. Data analysis was made using one-way analysis of variance, Tamhane post hoc test, and Weibull statistics (α = 0.05). Results: Significant differences were observed among the groups for the fracture load (p < 0.0001). MM frameworks showed the highest fracture load values. The PEEK group registered higher fracture load values than zirconia samples. The Weibull statistics corroborated these results. The fracture pattern was different among the groups. Conclusions: Milled metal provided the highest fracture load values, followed by PEEK, and zirconia. However, all tested groups demonstrated clinically acceptable fracture load values higher than 1000 N. PEEK might be considered a promising alternative for posterior FPDs.
Concrete Examination of 100-Year-Old Bridge Structure above the Kłodnica River Flowing through the Agglomeration of Upper Silesia in Gliwice: A Case Study
Authors: Słomka-Słupik Barbara , Podwórny Jacek , Grynkiewicz-Bylina Beata , Salamak Marek , Bartoszek Bibianna , Drzyzga Wiktoria , Maksara Marcel
Published: February 2021

The article analyzes the composition of concrete taken from various elements from a 100-year-old bridge in South Poland, so as to analyze its technical condition. The main methods applied during experimental work were: Designation of pH, free chloride content, salinity, XRD and SEM examinations, as well as metals determination using inductively coupled plasma mass spectrometry (ICP­MS), high-performance liquid chromatography (HPLC)-ICP-MS, and cold-vapor atomic absorption spectroscopy (CV-AAS). The concrete of the bridge was strongly carbonated and decalcified with an extremely high content of chlorides. The pH of the concrete was in a range from 10.5 to 12.0. Acid soluble components were between 9.9% and 17.6%. Typical sulfate corrosion phases of concrete were not detected. Friedels’ salt was found only at the extremity of an arch. The crown block was corroded to the greatest extent. Various heavy metals were absorbed into the concrete, likely from previous centuries, when environmental protection policy was poor. The applied research methodology can be used on bridges exposed to specific external influences. The acquired knowledge can be useful in the management processes of the bridge infrastructure. It can help in making decisions about decommissioning or extending the life cycle of the bridge. This work should also sensitize researchers and decision-makers to the context of “bridge safety”.
An Improved Nordhaus–Gaddum-Type Theorem for 2-Rainbow Independent Domination Number
Authors: Zhu Enqiang
Published: February 2021

For a graph G, its k-rainbow independent domination number, written as γrik(G), is defined as the cardinality of a minimum set consisting of k vertex-disjoint independent sets V1,V2,…,Vk such that every vertex in V0=V(G)\(∪i=1kVi) has a neighbor in Vi for all i∈{1,2,…,k}. This domination invariant was proposed by Kraner Šumenjak, Rall and Tepeh (in Applied Mathematics and Computation 333(15), 2018: 353–361), which aims to compute the independent domination number of G□Kk (the generalized prism) via studying the problem of integer labeling on G. They proved a Nordhaus–Gaddum-type theorem: 5≤γri2(G)+γri2(G¯)≤n+3 for any n-order graph G with n≥3, in which G¯ denotes the complement of G. This work improves their result and shows that if G≇C5, then 5≤γri2(G)+γri2(G¯)≤n+2.
Robust Sensorless Model-Predictive Torque Flux Control for High-Performance Induction Motor Drives
Authors: Aziz Ahmed G. Mahmoud A. , Rez Hegazy , Diab Ahmed A. Zaki
Published: February 2021

This paper introduces a novel sensorless model-predictive torque-flux control (MPTFC) for two-level inverter-fed induction motor (IM) drives to overcome the high torque ripples issue, which is evidently presented in model-predictive torque control (MPTC). The suggested control approach will be based on a novel modification for the adaptive full-order-observer (AFOO). Moreover, the motor is modeled considering core losses and a compensation term of core loss applied to the suggested observer. In order to mitigate the machine losses, particularly at low speed and light load operations, the loss minimization criterion (LMC) is suggested. A comprehensive comparative analysis between the performance of IM drive under conventional MPTC, and those of the proposed MPTFC approaches (without and with consideration of the LMC) has been carried out to confirm the efficiency of the proposed MPTFC drive. Based on MATLAB® and Simulink® from MathWorks® (2018a, Natick, MA 01760-2098 USA) simulation results, the suggested sensorless system can operate at very low speeds and has the better dynamic and steady-state performance. Moreover, a comparison in detail of MPTC and the proposed MPTFC techniques regarding torque, current, and fluxes ripples is performed. The stability of the modified adaptive closed-loop observer for speed, flux and parameters estimation methodology is proven for a wide range of speeds via Lyapunov’s theorem.
DNA-Guided Assembly for Fibril Proteins
Authors: Amărioarei Alexandru , Spencer Frankie , Barad Gefry , Gheorghe Ana-Maria , Iţcuş Corina , Tuşa Iris , Prelipcean Ana-Maria , Păun Andrei , Păun Mihaela , Rodriguez-Paton Alfonso , Trandafir Romică , Czeizler Eugen
Published: February 2021

Current advances in computational modelling and simulation have led to the inclusion of computer scientists as partners in the process of engineering of new nanomaterials and nanodevices. This trend is now, more than ever, visible in the field of deoxyribonucleic acid (DNA)-based nanotechnology, as DNA’s intrinsic principle of self-assembly has been proven to be highly algorithmic and programmable. As a raw material, DNA is a rather unremarkable fabric. However, as a way to achieve patterns, dynamic behavior, or nano-shape reconstruction, DNA has been proven to be one of the most functional nanomaterials. It would thus be of great potential to pair up DNA’s highly functional assembly characteristics with the mechanic properties of other well-known bio-nanomaterials, such as graphene, cellulos, or fibroin. In the current study, we perform projections regarding the structural properties of a fibril mesh (or filter) for which assembly would be guided by the controlled aggregation of DNA scaffold subunits. The formation of such a 2D fibril mesh structure is ensured by the mechanistic assembly properties borrowed from the DNA assembly apparatus. For generating inexpensive pre-experimental assessments regarding the efficiency of various assembly strategies, we introduced in this study a computational model for the simulation of fibril mesh assembly dynamical systems. Our approach was based on providing solutions towards two main circumstances. First, we created a functional computational model that is restrictive enough to be able to numerically simulate the controlled aggregation of up to 1000s of elementary fibril elements yet rich enough to provide actionable insides on the structural characteristics for the generated assembly. Second, we used the provided numerical model in order to generate projections regarding effective ways of manipulating one of the the key structural properties of such generated filters, namely the average size of the openings (gaps) within these meshes, also known as the filter’s aperture. This work is a continuation of Amarioarei et al., 2018, where a preliminary version of this research was discussed.
Inflammatory Undifferentiated Pleomorphic Sarcoma Mimicking Bacteremia in an Elderly Patient: A Case Report
Authors: Hashimoto Kazuhiko , Nishimura Shunji , Ito Tomohiko , Oka Naohiro , Akagi Masao
Published: February 2021

Undifferentiated pleomorphic sarcoma (UPS) is major type of soft tissue sarcomas. UPS presenting with inflammation is rare, and its pathophysiology remains unclear. Herein, we report a rare case of UPS with prolonged fever. A 91-year-old female complaining of high fever was referred to our hospital because of a high C-reactive protein (CRP) level of 12.51 mg/dL. She had been experiencing intermittent fevers for approximately 10 years. The fever of unknown origin worsened with time and went into remission with repeated antimicrobial therapy. She also had a mass on her central lower back over the sacral region for 6 years, which showed a gradual increase in size. The blood tests showed that the leukocyte count and neutrophils were 6.51 × 103 /µL and 70.3%, respectively. She had a 10 × 10 cm mass on her buttock that showed 2-[fluorine-18] fluoro-2-deoxy-d-glucose (FDG) accumulation on FDG-positron emission tomography-computed tomography examination (standardized uptake value-max value: 5.4). A blood culture examination was performed to rule out bacteremia, however, no bacteria were identified. We then performed a needle biopsy and confirmed the diagnosis of UPS; subsequently, the patient underwent a wide-margin resection. A few days after the surgery, her CRP, leukocyte, and neutrophil levels decreased to 0.305 mg/dL, 2.83 × 103/uL, and 50.1%, respectively. This case demonstrated that UPS with inflammation should be treated surgically as soon as possible after ruling out other sources of infection to achieve a favorable prognosis.
Improvement of Fermentation Quality in the Fermented Total Mixed Ration with Oat Silage
Authors: Yang Hong , Wang Bing , Zhang Qing , Cheng Hui , Yu Zhu
Published: February 2021

The use of the fermented total mixed ration (FTMR) is a promising approach for the preservation of homogeneous feed, but changes during fermentation and links with the bacterial community of FTMR are not fully understood. This study investigated the effects of adding oat silage (OS) to the fermented total mixed ration (FTMR) in terms of fermentation, chemical composition, and the bacterial community. The fermentation quality of FTMR with 22% OS was greatly improved, as demonstrated by decreases in the butyric acid concentration, a lower lactic acid/acetic acid ratio, a larger population of lactic acid bacteria (LAB), and quicker spoilage yeast death. Further examination of the effects of various ensiling days on nutritive values showed stable crude protein and nonprotein nitrogen (NPN) contents. The concentrations of acetic acid, propionic acid, and ammonia–nitrogen (NH3–N) were increased following all FTMR treatments after 15 d, while the concentration of water-soluble carbohydrates (WSC) was decreased. More heterofermentative LAB, such as Lentilactobacillus buchneri, Lentilactobacillus brevis, and Companilactobacillus versmoldensis were found after adding 11% and 22% OS. Moreover, the addition of 22% OS caused a marked increase in both bacterial richness and diversity, dominated by the Lactobacillus genus complex. Among species of the Lactobacillus genus complex, the occurrence of Loigolactobacillus coryniformis was positively correlated with lactic acid, NPN, and NH3–N concentrations, suggesting its potential role in altering the fermentation profiles.
New Insights into the Co-Occurrences of Glycoside Hydrolase Genes among Prokaryotic Genomes through Network Analysis
Authors: Geng Alei , Jin Meng , Li Nana , Zhu Daochen , Xie Rongrong , Wang Qianqian , Lin Huaxing , Sun Jianzhong
Published: February 2021

Glycoside hydrolase (GH) represents a crucial category of enzymes for carbohydrate utilization in most organisms. A series of glycoside hydrolase families (GHFs) have been classified, with relevant information deposited in the CAZy database. Statistical analysis indicated that most GHFs (134 out of 154) were prone to exist in bacteria rather than archaea, in terms of both occurrence frequencies and average gene numbers. Co-occurrence analysis suggested the existence of strong or moderate-strong correlations among 63 GHFs. A combination of network analysis by Gephi and functional classification among these GHFs demonstrated the presence of 12 functional categories (from group A to L), with which the corresponding microbial collections were subsequently labeled, respectively. Interestingly, a progressive enrichment of particular GHFs was found among several types of microbes, and type-L as well as type-E microbes were deemed as functional intensified species which formed during the microbial evolution process toward efficient decomposition of lignocellulose as well as pectin, respectively. Overall, integrating network analysis and enzymatic functional classification, we were able to provide a new angle of view for GHs from known prokaryotic genomes, and thus this study is likely to guide the selection of GHs and microbes for efficient biomass utilization.
Novel Complex of PD-L1 Aptamer and Holliday Junction Enhances Antitumor Efficacy in Vivo
Authors: Li Ting , Yao Fengjiao , An Yacong , Li Xundou , Duan Jinhong , Yang Xian-Da
Published: February 2021

Blocking the PD-1/PD-L1 pathway can diminish immunosuppression and enhance anticancer immunity. PD-1/PD-L1 blockade can be realized by aptamers, which have good biocompatibility and can be synthesized in quantity economically. For in vivo applications, aptamers need to evade renal clearance and nuclease digestion. Here we investigated whether DNA nanostructures could be used to enhance the function of PD-L1 aptamers. Four PD-L1 aptamers (Apt) were built into a Holliday Junction (HJ) to form a tetravalent DNA nanostructure (Apt-HJ). The average size of Apt-HJ was 13.22 nm, which was above the threshold for renal clearance. Apt-HJ also underwent partial phosphorothioate modification and had improved nuclease resistance. Compared with the monovalent PD-L1 aptamer, the tetravalent Apt-HJ had stronger affinity to CT26 colon cancer cells. Moreover, Apt-HJ markedly boosted the antitumor efficacy in vivo vs. free PD-L1 aptamers without raising systemic toxicity. The results indicate that multiple aptamers attached to a DNA nanostructure may significantly improve the function of PD-L1 aptamers in vivo.
Suppression of Rice Cryptochrome 1b Decreases Both Melatonin and Expression of Brassinosteroid Biosynthetic Genes Resulting in Salt Tolerance
Authors: Hwang Ok Jin , Back Kyoungwhan
Published: February 2021

We investigated the relationship between the blue-light photoreceptor cryptochrome (CRY) and melatonin biosynthesis by generating RNA interference (RNAi) transgenic rice plants that suppress the cryptochrome 1b gene (CRY1b). The resulting CRY1b RNAi rice lines expressed less CRY1b mRNA, but not CRY1a or CRY2 mRNA, suggesting that the suppression is specific to CRY1b. The growth of CRY1b RNAi rice seedlings was enhanced under blue light compared to wild-type growth, providing phenotypic evidence for impaired CRY function. When these CRY1b RNAi rice plants were challenged with cadmium to induce melatonin, wild-type plants produced 100 ng/g fresh weight (FW) melatonin, whereas CRY1b RNAi lines produced 60 ng/g FW melatonin on average, indicating that melatonin biosynthesis requires the CRY photoreceptor. Due to possible feedback regulation, the expression of melatonin biosynthesis genes such as T5H, SNAT1, SNAT2, and COMT was elevated in the CRY1b RNAi lines compared to the wild-type plants. In addition, laminar angles decreased in the CRY1b RNAi lines via the suppression of brassinosteroid (BR) biosynthesis genes such as DWARF. The main cause of the BR decrease in the CRY1b RNAi lines seems to be the suppression of CRY rather than decreased melatonin because the melatonin decrease suppressed DWARF4 rather than DWARF.
Molecular Targeting of Epidermal Growth Factor Receptor (EGFR) and Vascular Endothelial Growth Factor Receptor (VEGFR)
Authors: Kaufman Nichole E. M. , Dhingra Simran , Jois Seetharama D. , Vicente Maria da Graça H.
Published: February 2021

Epidermal growth factor receptor (EGFR) and vascular endothelial growth factor receptor (VEGFR) are two extensively studied membrane-bound receptor tyrosine kinase proteins that are frequently overexpressed in many cancers. As a result, these receptor families constitute attractive targets for imaging and therapeutic applications in the detection and treatment of cancer. This review explores the dynamic structure and structure-function relationships of these two growth factor receptors and their significance as it relates to theranostics of cancer, followed by some of the common inhibition modalities frequently employed to target EGFR and VEGFR, such as tyrosine kinase inhibitors (TKIs), antibodies, nanobodies, and peptides. A summary of the recent advances in molecular imaging techniques, including positron emission tomography (PET), single-photon emission computerized tomography (SPECT), computed tomography (CT), magnetic resonance imaging (MRI), and optical imaging (OI), and in particular, near-IR fluorescence imaging using tetrapyrrolic-based fluorophores, concludes this review.