bluuebunny/crossref_metadata_2025 huggingface dataset:
ESTUDO COMPARATIVO DO CONTROLE DA QUALIDADE DE COMPRIMIDOS DE NIMESULIDA COMPLEXADA E NÃO COMPLEXADA COM Β-CICLODEXTRINA
Authors: Jesus Mariana de Oliveira , Moraes Vitória Alves de , Lima Bruno dos Santos , Menezes Paula dos Passos
Published: December 2020

O controle da qualidade de sólidos por meio de ensaios físico-químicos apresenta grande importância na garantia da qualidade de medicamentos. Diante disto, este estudo teve como objetivo comparar a qualidade de comprimidos de nimesulida com β-ciclodextrina (Maxsulid®) e sem β-ciclodextrina (Nisulid®) como forma de investigar se os testes vigentes se aplicam às formas farmacêuticas que contenham complexos de inclusão com ciclodextrinas em sua composição. Para tal, foram realizados ensaios físico-químicos descritos na Farmacopeia Brasileira 6ª edição para comprimidos de nimesulida. Frente ao exposto, ambos os medicamentos obtiveram resultados satisfatórios na maioria dos testes de caracterização físico-química. Os testes de doseamento e dissolução foram realizados por meio de determinações quantitativas utilizando a Cromatografia Líquida de Alta Eficiência. Os resultados do doseamento mostraram que a metodologia aplicada não está adequada para extrair o fármaco do complexo de inclusão e assim doseá-lo de maneira eficiente. No teste de dissolução, os dois medicamentos foram aprovados de acordo com os critérios estabelecidos na Farmacopeia Brasileira. Porém, Maxsulid® foi o único a obter sua totalidade dissolvida em 120 minutos. Dessa forma, fica clara a importância da implementação de protocolos analíticos adequados às novas tecnologias aplicadas na produção de medicamentos.
PROBLEMAS RELACIONADOS AOS MEDICAMENTOS EM PACIENTES ATENDIDOS NO PRONTO SOCORRO DE UM HOSPITAL UNIVERSITÁRIO
Authors: Da Cruz Paulimira Fernanda , Paiva Amanda de Cássia , Júnior Miguel Simião Pereira , Muniz Jaqueline Jóice
Published: December 2020

Problemas relacionados aos medicamentos (PRM) são causados por falhas que afetam a efetividade e segurança dos medicamentos e representam um importante desafio para os sistemas de saúde. Este estudo teve por objetivo identificar e avaliar casos de PRM no Pronto Socorro (PS) do Hospital das Clínicas Samuel Libânio (HCSL). Trata-se de um estudo descritivo e transversal, realizado por meio de entrevistas a pacientes que procuraram atendimento no PS do HCSL. A identificação e classificação dos PRM foram baseadas no Segundo Consenso de Granada. Dos 100 pacientes entrevistados, houve alta prevalência de PRM no grupo de pacientes acima de 60 anos (46%), porém não houve diferença significativa entre sexo feminino (24%) e masculino (22%). Grande parte dos entrevistados teve pouca adesão ao tratamento (68%) e nível de escolaridade baixo, sem ensino fundamental (62%). Entre os avaliados, todos apresentaram algum tipo de PRM, sendo os mais encontrados: inefetividade quantitativa (78%) e problema de saúde não tratado (16%). Os casos de PRM atendidos no PS do HCSL são comuns em pacientes com doenças crônicas, com idade acima de 60 anos, devido a não adesão ao tratamento. Esses resultados demonstram a importância da implantação do seguimento farmacoterapêutico pautado na educação e atenção primária à saúde.
Protein molecular defect detection method based on a neural network algorithm
Authors: Zheng Meiqing , Kahrizi Somayeh
Published: December 2020

Proteins, as the largest macromolecules in the body, are among the most important components of the body and play very vital and important roles. These substances are made up of a series of amino acid chains that, depending on the type of protein, the number of these amino acids can reach several thousand. Proteins function differently depending on the type and location of their presence, including enzymatic activity to catalyze the process, identify microbes and cancer cells, transport substances such as respiratory gases, and signalize. In the biochemical experiment, the problem of optimizing the detection of protein molecular defects, because of the randomness of the information, parameters, selection and setting, limits the detection accuracy of protein molecular defects. Based on the characteristics of fast learning speed and a robust network of neural network algorithm, a protein molecular defect detection method based on a neural network algorithm was proposed. Firstly, the protein secondary structure was predicted by the method of protein secondary structure prediction based on the generalized regression neural network to obtain the protein structural features; secondly, the protein defective molecular sequence classification model based on the neural network was used to classify the protein defective molecular sequence to achieve the protein molecular defect detection. The results showed that the detection accuracy of the proposed method was very high, which meets the needs of protein molecular defect detection, and has some application advantages compared with similar detection methods.
Quercetin may repair the impaired oxidant-antioxidant balance in 1, 2, 3, 4-tetrachlorodibenzo-p-dioxin induced cardiac injury in male rats
Authors: Nanakali Nadir Mustafa
Published: December 2020

The purpose of this study was to evaluate the prophylactic effects of quercetin (QUE) against oxidative damages and histological changes induced by 1, 2, 3, 4-tetrachlorodibenzo-p-dioxin (TCDD) in rat heart. For this purpose, 20 adult male Wistar rats were divided into four groups as fellow control group, TCDD group (10µg/kg BW/day by gavage daily for 60 consecutive days), QUE group (30 mg/kg BW/day) and TCDD + QUEgroup. The oxidative biomarkers of heart tissue and the levels of cTnI and lipid profile in serum were measured. Our results showed that the cTnI serum level and the heart lipid peroxidation significantly increased and the heart level of antioxidant profile significantly decreased in the TCDD group compared to control and QUEgroups. While pretreatment with QUEcould significantly improved these factors in serum and heart tissue in animals that consumed TCDD. It can be concluded that QUEat doses of 30 mg/kg/day could alleviate heart oxidative damage and histological changes induced by TCDD.
Potential risk assessment of miR-143 gene polymorphisms rs41291957 and rs353292 in colorectal cancer
Authors: Ahmed Mehvish Naseer , Mansoor Qaisar , Baig Ruqia Mehmood , Tahir Tehreem , Abbasi Samina Asghar
Published: December 2020

Colorectal cancer is a life-threatening and therapeutically challenging disease. Increasingly it is being deciphered that genetic and epigenetic mutations play a central role in cancer onset and progression. Excitingly, discovery of non-coding RNAs is considered to be a milestone in molecular oncology and emerging evidence is deepening our understanding about pivotal role of miRNAs in carcinogenesis. miR-143 has been experimentally verified to play an instrumental role as tumor suppressor. Recent studies suggest that single nucleotide polymorphisms rs41291957 and rs353292 in miR-143 may associate with the progression and or development of colorectal cancer. In present study 400 Pakistani subjects participated including 200 colorectal cancer patients and 200 age and gender matched healthy individuals. Blood samples and clinical information of the confirmed patients was collected from cancer diagnosis and treatment hospitals in Pakistan. The polymorphisms rs41291957 and rs353292 were genotyped in patients and controls by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and results were validated by Sanger sequencing. The association of the SNPs within the study group was analyzed by Ï‡² test with p value < 0.05 as significant. Odds ratio was calculated with 95% confidence interval.Genetic predisposition to cancer was observed in presence of characteristic rs45291957 polymorphism. Ï‡² test results show strongly significant association mi-RNA rs45291957 SNP with colorectal cancer p value 0.0111 (<0.05) along with the statistically significant correlation tested by odds ratio with 95% confidence interval. However, no significant correlation (p value 0.6683) could be found for the association of rs353292 with colorectal cancer in Pakistani population. The present study for the first time gave evidence of miR-143 rs41291957 involvement in colorectal cancer patients of Pakistani population. This target can be a useful molecular tool for the prognosis and treatment targets for colorectal cancer in Pakistani population. rs353292 genetic association can be explored for different cancers in Pakistan to completely rule out its role in cancer.
Raddeanin A suppresses lung cancer cell proliferation via induction of apoptosis and increased production of ROS
Authors: Jin Weiguo , Lu Shichun , Wang Xiaolin , Shu Yusheng , Shi Hongcan
Published: December 2020

At present, in vitro cell experiments have confirmed that RaddeaninA can effectively inhibit the proliferation of some tumor cells, but the effect of RaddeaninA on lung cancer cells has not been observed. Therefore, this study explored its effect on lung cancer cells and its mechanism of action. Human lung cancer cell lines were treated with serum-free medium and varied concentrations of Raddeanin A. Cell proliferation and apoptosis were determined using MTT, and flow cytometric assays, respectively. The intracellular level of ROS was determined using DCFH-DA assay. Protein and mRNA expressions of bax, bcl-2 and cyt c were measured using Western blotting and qRT-PCR. RaddeaninA treatment can promote PC-9 cell apoptosis in a time and dose-dependent manner (p<0.05). Treatment of PC-9 cells with Raddeanin significantly and dose-dependently increased the activities of caspase-9 and caspase-3 (p<0.05), and led to significant and dose-dependent increases in ROS levels (p<0.05). Treatment of PC-9 cells with Raddeanin A led to significant and dose-dependent decreases in mitochondrial membrane potential (p<0.05). It significantly and dose-dependently upregulated bax mRNA and protein expressions, but down-regulated bcl-2 mRNA and protein expressions significantly and dose-dependently (p<0.05). On the other hand, Raddeanin significantly and dose-dependently down-regulated cytoplasmic bax protein expression, while upregulating cyt c expression (p<0.05). Similarly, bax protein expression was significantly and dose-dependently upregulated in mitochondria, but the corresponding cyt c expression was significantly and dose-dependently down-regulated (p<0.05). Raddeanin A is a potential and effective lung cancer chemotherapy drug, which can induce lung cancer cell apoptosis and inhibit proliferation.
miR-196a targeting LRIG3 promotes the proliferation and migration of cervical cancer cells
Authors: Qiu Yunfen , Han Qing , Lu Hong , Shi Chunming
Published: December 2020

In recent years, studies have found that miR-RNA plays a role in cell proliferation, differentiation, apoptosis and metabolism. Among them, miR-196a is closely related to cervical cancer. Therefore, this experiment investigates the effect of mir-196a expression on cervical cancer cells and related mechanisms. The expression level of miR-196a in the cervical cancer cell line was assayed with the RT-PCR method, and liposome transfection was used to investigate its up-regulation or down-regulation in cervical cancer cells. The CCK-8 method and flow cytometry were used to measure cervical cancer cell proliferation and apoptosis, while the Transwell assay was used to determine cell migration and invasion of each transfection group. Bioinformatics was used to predict the target gene of miR-196a, which was verified using dual luciferase report experiment and Western blot, and miR-196a was further transfected with si-LRIG3 to detect its reversal effect on miR-196a regulation. Inhibition of the expression of miR-196a significantly reduced the proliferation, migration and invasion of cervical cancer cells, and promoted their apoptosis. Results from dual luciferase assay showed that miR-196a and LRIG3 had direct targeting effects. Cell proliferation, migration and invasion were enhanced by a reduction in the expression level of LRIG3 protein after miR-196a inhibitor cells were transfected with si-LRIG3. The expression of miR-196a is up-regulated in cervical cancer, and it promotes the growth of cervical cancer by its targeting effect on LRIG3 expression, resulting in enhancement of the proliferation, migration and invasion ability of cervical cancer cells, and inhibition of apoptosis.
miR-409-3p inhibits the proliferation and migration of human ovarian cancer cells by targeting Rab10
Authors: Li Yanyi , Chen Li , Zhang Beibei , Ohno Yuko , Hu Hua
Published: December 2020

Ovarian cancer is a leading cause of gynecological cancer-related mortality. It has been reported that miR-409-3p is involved in the proliferation and migration of cancer cells. However, the role of miR-409-3p in ovarian cancer has not been well studied. The present study aimed to investigate the functional role of miR-409-3p in the pathogenesis of ovarian cancer, and its potential mechanism. It was found that the expression levels of miR-409-3p in 6 ovarian cancer tissues were upregulated. Through proliferation, migration and colony formation assays, it was revealed that overexpression of miR-409-3p inhibited the proliferation and migration of ovarian cancer cells. It was predicted from bioinformatics assays that the complementary binding sites were within miR-409-3p and Rab10. It was also demonstrated that the downregulation of the expression of Rab10 reversed the miR-409-3p downregulation-induced abnormal proliferation of ovarian cancer cells. These results suggest that miR-409-3p expression can be used as a predictive marker for the prognosis of ovarian cancer. Thus, the miR-409-3p/Rab10 axis may be a novel therapeutic target for ovarian cancer.
Lymphocyte cell population as a potential hematological index for early diagnosis of COVID-19
Authors: Zhu Yihua , Cao XingJian , Lu Yonghui , Xu Dongsheng , Lu Renfei , Li Xinling
Published: December 2020

The pandemic diseases caused by SARS-CoV-2 are now threatening human health and survival. Early diagnosis and isolation of mild or asymptomatic COVID-19 patients is important to control the spread of SARS-CoV-2. In this study, we investigate the potential clinical utility of lymphocyte CPD for early diagnosis of COVID-19. To investigate the potential of lymphocyte cell population data (lymphocyte CPD) for use in early diagnosis of coronavirus disease 2019 (COVID-19). Lymphocyte CPD of healthy control (n = 51), common cold patients (n = 49) and mild COVID-19 patients (n = 126) were generated using hematology analyzer. The parameters were subjected to sensitivity and specificity analysis to determine their suitability as biomarkers for early diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Normality analysis showed that lymphocyte CPD followed a normal distribution. There were no significant differences in white blood cells (WBC) and lymphocyte (LY#) counts as well as the neutrophil-to-lymphocyte ratio (NLR) among the groups (p > 0.05). Lymphocyte volume standard deviation (LV-SD), lymphocyte conductivity standard deviation (LC-SD) and lymphocyte light scatter standard deviation (LS-SD) were significantly higher in the COVID-19 group than in common cold and control groups (p < 0.05). The corresponding mean lymphocyte light scattering  (MLS) was significantly reduced in the COVID-19 group, relative to the common cold group, but was significantly increased, when compared with the control group (p < 0.05). Moreover, there was no significant difference in mean lymphocyte volume (MLV) between the COVID-19 group and the common cold or control group (p > 0.05), but it was significantly higher in the common cold group than in the control group (p < 0.05). At a cutoff value â‰¥ 16.38, LS-SD was more sensitive and specific than other lymphocyte CPD parameters. At a cutoff value â‰¥ 11.89, LC-SD achieved 84.4 % sensitivity, 87.5 % specificity, and an area under the curve (AUC) of 0.888. However, at a cutoff value â‰¥ 15.95, LS-SD reached 81.3 % sensitivity, 75 % specificity and an AUC of 0.876. These results suggest that lymphocyte CPD parameters have great diagnostic potential for SARS-CoV-2 infection and can be used for early diagnosis of the disease.
Multi-omics analysis profile oral tumor module clusters to reveal the potential pathogenic mechanism
Authors: Liu Jun , Zhao Chaoyue , Yang Song , Dong Chen
Published: December 2020

Oral tumors are malignant cancers caused by abnormal proliferation or pathological changes of soft or hard tissues in the oral cavity. Serious cases may pose a threat to life. However, its precancerous lesions remain unclear. This study is based on a comprehensive strategy to explore a multi-factor-driven oral cancer barrier module, which is an attempt to describe the pathogenesis of the disease and potential regulatory drugs from a global perspective. Functional disease modules were identified by constructing a protein-specific interaction network in patients' oral tissues. Then, comprehensive pathogenesis was explored through combination with analysis of functional and signaling pathway enrichment, prediction of key regulatory factors. It was found that these specifically expressed proteins and their interactions often play a pivotal part in oral tumors. This is reflected in the results of functional and pathway enrichment of modulating genes, which show that they are mainly involved in various immune responses, inflammatory reactions, oral plaque, and oral ulcer-related regulatory processes. This may represent the potential pathogenesis of oral tumors. On the predictive analysis of regulators, a series of ncRNAs (including miR-590, CRNDE and miR-340) and transcription factors (including E2F1, MYC and TP53) were identified that have potential important regulatory effects on oral tumors. These key regulators may manipulate a crucial part of the module sub-network and then work together to mediate the occurrence of oral tumors. On the comprehensive Multi-omics module analysis, the specific proteins and their interactions in patients' oral tissues were identified, while the prominent pivotal regulators were involved in the different pathogenic functions of oral tumors.
Development of poly ADP-ribose polymerase-1 inhibitor with anti-cervical carcinoma activity
Authors: Zhang Tong-Mei , Wang Wen
Published: December 2020

This research aimed to discover and identify new poly ADP-ribose polymerase-1 (PARP) inhibitors with potent anti-cervical carcinoma activity, and then explore their potential biological roles on cervical carcinoma cell. For this purpose, we identified a new PARP inhibitor from a high-throughput virtual screening method and found that the compound strongly inhibited cervical carcinoma HeLa cell. Cell proliferation was evaluated by an MTT assay, and the cell apoptosis was assessed by flow cytometry. Results showed that PARP1 is a poly ADP-ribose catalyzing enzyme in eukaryotic cells, which is activated during DNA damage and repair, and plays an important role in DNA repair and cell apoptosis. Herein we report the first discovery of a new PARP inhibitor from a high-throughput virtual screening method, then the compound was measured its anti-cervical carcinoma activity by using an MTT assay, which suggested that the compound strongly inhibited HeLa cell proliferation, the IC50 value is 0.65 µM. In addition, the compound induced HeLa cell apoptosis in a dose-response manner. All these data suggested that the compound is a promising lead compound, which deserves further investigation. It is concluded that the compound discover herein is a promising PARP-1 inhibitor with potent anti-cervical carcinoma activity, which deserves further investigation.
The effect of sevoflurane on the spatial recall ability and expression of apolipoprotein E and Î² amyloid in the hippocampus in rats
Authors: Jiang Wanwei , Wang Qinghui , Yajing Liao Yajing Liao , Sun Yue , Yang Rui
Published: December 2020

This study aimed to observe the recent spatial recall ability and the changes of expression of hippocampal apolipoprotein E (ApoE) and amyloid Î² protein (AÎ²) in adult rats after inhaling sevoflurane anesthetic drugs, and to analyze the mechanism of action. For this purpose, a total of 54 adult SD clean-grade rats were selected in this study and were randomly divided into the sevoflurane anesthesia group, carrier gas group and control group, 18 rats in each group. The rats in the carrier gas group were inhaled with 1 L/min of oxygen O2+1 L/min air mixed carrier gas for 2 h, and the rats in the sevoflurane anesthesia group were given 3.2%sevoflurane for 2 hours based on the carrier gas group, the control rats were naturally reared. Before the model was copied, the Morris water maze experiment was performed before the material was taken. Some rat brain tissues were extracted on the first day (T1), the third day (T3), and the seventh day (T7) after model replication. The immunohistochemistry was used to measure the mean optical density (MOD) value of APOE and AÎ² in hippocampal CA1, CA3 and DG regions. The indicators above at different time points of each group were compared and analyzed. Results showed that the number of crossing the original platform at each time point, the residence time of the original platform quadrant, the number of entering the original platform quadrant, and the percentage of the original platform quadrant residence time in the sevoflurane anesthesia group and the carrier gas group were compared, and there were no significant differences between two groups (P>0.05). Compared with the carrier gas group, the MOD values of APOE in the hippocampus at T1 and T3 time points in the sevoflurane anesthesia group were decreased (P<0.05), the MOD values of AÎ² in the hippocampus at the T7 time point were increased (P<0.05). It concluded that Inhalation of 3.2%sevoflurane has no obvious damage to the recent spatial recall ability of adult rats. Within 7 days after inhalation of 3.2% sevoflurane, it can inhibit hippocampus AÎ² deposition through down-regulating APOE expression level. The critical time point for hippocampal AÎ² increasing was 7 days after anesthesia.
Scaffolding in second language teaching. The teaching unit “The poem ‘Der Herbst steht auf der Leiter’ by Peter Hacks“
Authors: Schulz-Andrzejewska Joanna
Published: December 2020

This article presents a lesson plan for German taught as a second language in Middle School. The plan shows the creative integration of a poem by Peter Hacks into the lesson. The artful and partly three-dimensional work improves the understanding of the text and benefits the student’s feeling for the language. The students are guided to the underlying objective through different tasks. In the end, they memorize the poem and present it in front of the class.
Antropolingwistyka: przegląd problematyki
Authors: Zaniewski Jan , Nizhneva Natallia
Published: December 2020

Anthropolinguistics is one the most recent theoretical and practical perspectives developed within the science of linguistics. As such, it focus on the central question of the evolution of human thinking and its reflection in language. It is proposed that on the basis of evolutionary processes, it is possible to put forth a view according to which any further development of humanity depends on the presence and steady development of specialist lexicon. It secures the advancement of humanity in science, industry and culture.
Wschodniosłowiańskie i polskie pieśni tradycyjne jako uniwersalny nośnik kultury
Authors: Braszak Piotr
Published: December 2020

In the presented article, the author considers a rarely discussed subject of traditional song lyrics perceived as culture transmitters. Even though they are very universal and comprehensive sources in terms of cultural transmission, academics use them in scientific discourse incommensurately less frequently than other textual forms or art works from the epoch. The author analyzes traditional folk songs as texts which unfold before the reader. Their crucial value and broad possibility to understand different and interfusing realms of human activity associated with Polish and East Slavic culture are presented.
Design of Cryogenic Storage Tanks for Industrial Applications
Authors: Marsh Herbert W.
Published: December 2020

This is a discussion of the facets of cryogenic storage tank design directed toward those who have only limited experience in the field. Design considerations as to cost, suitability of materials for the temperatures and pressures involved, configuration of inner vessels and jackets, support systems, and types of insulation, evacuated and nonevacuated, for both shop-built and fielderected vessels are discussed in brief. The potential requirements for cryogenic storage for industrial applications are listed. Military applications for both ground and air-borne use are excluded as these involve unusual design conditions foreign to the usual industrial installation.
NousRire : étude de cas d’une entreprise québécoise repensant la marque responsable traditionnelle
Authors: Girard Audrey , Deschênes Jonathan
Published: December 2020

Fondée en 2015, NousRire est une petite entreprise à but lucratif québécoise qui a pour mission de « rendre accessibles des aliments biologiques non périssables d’excellente qualité, tout en permettant de faire des économies et en créant un impact positif sur la Terre et sur ses habitants »1. L’entreprise offre aux clients de passer leurs commandes en ligne et de venir les récupérer dans des lieux de cueillette précis durant des périodes appelées « journées d’emballage ». Ces aliments sont disponibles en vrac pour minimiser la production de déchets. Plus de 500 bénévoles oeuvrent durant ces périodes afin de distribuer la nourriture dans les contenants des clients, qui emballent eux-mêmes leur commande. NousRire est constituée de 18 « cellules » autogérées ancrées dans diverses régions du Québec, dont l’Estrie, l’Abitibi, les Laurentides et la région de Montréal. L’entreprise fonctionne grâce à l’implication de 2000 bénévoles et d’environ 20 employés.
La consommation favorise-t-elle la mondialisation et ses conséquences?
Authors: Lemay Daniel
Published: December 2020

La mondialisation est un sujet polarisant. Elle a toujours eu ses défenseurs et ses détracteurs. Bien que nous parlions de mondialisation depuis plusieurs décennies, elle n’a jamais été autant d’actualité que depuis ces derniers mois. Puisque la pandémie de la COVID-19 l’a ramenée de façon soudaine à l’avant-scène des questionnements éthiques et politiques, le présent article a pour objectif de présenter deux ouvrages portant sur la consommation responsable et sur les échanges marchands, soit celui de l’économiste Dani Rodrick et celui des chercheurs en marketing Myriam Ertz, Damien Hallegatte et Julien Bousquet. L’article vise tout particulièrement à mettre au jour leurs racines similaires, soit la conscientisation de plus en plus grande des enjeux globaux de consommation et de leurs répercussions. Selon ces deux ouvrages, même si la mondialisation n’a pas concrétisé toutes les promesses attendues, ses retombées pourraient être plus équitables pour l’ensemble des parties prenantes des différents systèmes impliqués dans les échanges marchands.
Peripheral Myeloid Cell EP2 Activation Contributes to the Deleterious Consequences of Status Epilepticus
Authors: Varvel Nicholas H. , Espinosa-Garcia Claudia , Hunter-Chang Sarah , Chen Di , Biegel Ariel , Hsieh Allison , Blackmer-Raynolds Lisa , Ganesh Thota , Dingledine Raymond
Published: December 2020

A multidimensional inflammatory response ensues after status epilepticus (SE), driven partly by cyclooxygenase-2-mediated activation of prostaglandin EP2 receptors. The inflammatory response is typified by astrocytosis, microgliosis, erosion of the blood-brain barrier (BBB), formation of inflammatory cytokines, and brain infiltration of blood-borne monocytes. Our previous studies have shown that inhibition of monocyte brain invasion or systemic administration of an EP2 receptor antagonist relieves multiple deleterious consequences of SE. Here we identify those effects of EP2 antagonism that are reproduced by conditional ablation of EP2 receptors in immune myeloid cells and show that systemic EP2 antagonism blocks monocyte brain entry in male mice. The induction of hippocampal IL-6 after pilocarpine SE was nearly abolished in EP2 conditional KO mice. Serum albumin levels in the cortex, a measure of BBB breakdown, were significantly higher after SE in EP2-sufficient mice but not in EP2 conditional KOs. EP2 deficiency in innate immune cells accelerated the recovery from sickness behaviors following SE. Surprisingly, neurodegeneration was not alleviated in myeloid conditional KOs. Systemic EP2 antagonism prevented monocyte brain infiltration and provided broader rescue of SE-induced effects than myeloid EP2 ablation, including neuroprotection and broader suppression of inflammatory mediators. Reporter expression indicated that the cellular target of CD11b-driven Cre was circulating myeloid cells but, unexpectedly, not microglia. These findings indicate that activation of EP2 receptors on immune myeloid cells drives substantial deficits in behavior and disrupts the BBB after SE. The benefits of systemic EP2 antagonism can be attributed, in part, to blocking brain recruitment of blood-borne monocytes.

SIGNIFICANCE STATEMENTUnabated seizures reduce quality of life, promote the development of epilepsy, and can be fatal. We previously identified activation of prostaglandin EP2 receptors as a driver of undesirable consequences of seizures. However, the relevant EP2-expressing cell types remain unclear. Here we identify peripheral innate immune cells as a driver of the EP2-related negative consequences of seizures. Removal of EP2 from peripheral immune cells was beneficial, abolishing production of a key inflammatory cytokine, accelerating weight regain, and limiting behavioral deficits. These findings provide evidence that EP2 engagement on peripheral immune and brain endothelia contributes to the deleterious effects of SE, and will assist in the development of beneficial therapies to enhance quality of life in individuals who suffer prolonged seizures.
Affirmation of the Social Approach in the Case of Relocation of Community in Ciliwung Riverbank
Authors: Maring Prudensius , Prakosawati Elisabeth Endang
Published: December 2020

Urban development projects often lead to social contraction between the technical-physical approach and the socio-cultural approach. To explain the problem, this paper inspires the concepts of urban anthropology and socio-cultural development. The study was conducted in 2016-2017 in community located in Ciliwung riverbank, Jakarta. The methods used include in-depth interviews, participatory observations, and document analysis. The results of the study show that although river normalization projects and relocation projects have been equipped with a policy framework and technical framework that accommodates the interests of the community, the implementation of the social approach has not been implemented consistently. This condition raises doubts, rejections, and resistance from the community to maintain the social, economic and environmental resources that have been ruled on the riverbanks. This research confirms the importance of affirming socio-cultural approaches in urban development programs and projects that are carried out consistently by fulfilling the prerequisites for freedom in time, financing, and management resources.
Presurgical somatostatin receptor ligand treatment does not affect tumor consistency in GH-secreting pituitary macroadenomas
Authors: Araujo-Castro Marta , Pian Héctor , Ruz-Caracuel Ignacio , Acitores Cancela Alberto , Pascual-Corrales Eider , Rodríguez Berrocal Víctor
Published: December 2020

Purpose

To evaluate whether presurgical treatment using long-acting somatostatin receptor ligands (SRL) may change pituitary tumor consistency and improve surgical outcome in GH-secreting pituitary macroadenomas.

Methods

Retrospective study of 40 patients with GH-secreting pituitary macroadenomas operated for the first time by endoscopic transsphenoidal approach. Tumor consistency was evaluated intraoperatively and then correlated with histopathological fibrosis parameters and surgical outcomes. Surgical remission was reported based on the 2010 criteria.

Results

The mean tumor size of GH-secreting macroadenomas was of 16.9 ± 8.2 mm and 25 were invasive pituitary adenomas (PAs). Presurgical treatment with long-acting SRL was performed in 17 patients (11 lanreotide, 6 octreotide). The cure rate was higher in those patients pre-treated with monthly doses ≥30 mg of octreotide or ≥90 mg of lanreotide than in those treated with lower doses or untreated (8/11 (72.7%) vs 11/29 (37.9%), P = 0.049). However, although the proportion of soft tumors increased as higher doses of SRL were considered in the pre-treated group, no statistical significance was reached, even when the highest approved monthly doses were used (6/6 (100%) vs 23/34 (67.7%), P = 0.102). Moreover, we found that the remission rate was similar between fibrous and soft tumors (P = 0.873) and also of surgical complications (P = 0.859), despite of the higher prevalence of Knosp >2 (P = 0.035) and very large PA (P = 0.025) in fibrous tumors than in soft tumors.

Conclusions

Although presurgical treatment with high doses of SRL was associated with a 2.2-fold greater chance of surgical remission, this benefit was not related with changes in tumor consistency induced by the presurgical treatment.
Parathyroid hormone may play a role in the pathophysiology of primary hypertension
Authors: Oinonen Lasse , Tikkakoski Antti , Koskela Jenni , Eräranta Arttu , Kähönen Mika , Niemelä Onni , Mustonen Jukka , Pörsti Ilkka
Published: December 2020

Parathyroid hormone has been related with the risk of hypertension, but the matter remains controversial. We examined the association of parathyroid hormone with central blood pressure and its determinants in 622 normotensive or never-treated hypertensive subjects aged 19–72 years without diabetes, cardiovascular or renal disease, or cardiovascular medications. The methods were whole-body impedance cardiography and analyses of pulse wave and heart rate variability. Cardiovascular function was examined in sex-specific tertiles of plasma parathyroid hormone (mean concentrations 3.0, 4.3 and 6.5 pmol/L, respectively) during head-up tilt. Explanatory factors for haemodynamics were further investigated using linear regression analyses. Mean age was 45.0 (s.d. 11.7) years, BMI 26.8 (4.4) kg/m2, seated office blood pressure 141/90 (21/12) mmHg, and 309 subjects (49.7%) were male. Only five participants had elevated plasma parathyroid hormone and calcium concentrations. Highest tertile of parathyroid hormone presented with higher supine and upright aortic diastolic blood pressure (P < 0.01) and augmentation index (P < 0.01), and higher upright systemic vascular resistance (P < 0.05) than the lowest tertile. The tertiles did not present with differences in pulse wave velocity, cardiac output, or measures of heart rate variability. In linear regression analyses, parathyroid hormone was an independent explanatory factor for aortic systolic (P = 0.005) and diastolic (P = 0.002) blood pressure, augmentation index (P = 0.002), and systemic vascular resistance (P = 0.031). To conclude, parathyroid hormone was directly related to central blood pressure, wave reflection, and systemic vascular resistance in subjects without cardiovascular comorbidities and medications. Thus, parathyroid hormone may play a role in the pathophysiology of primary hypertension.
Treatment of malignancy-associated hypercalcemia with cinacalcet: a paradigm shift
Authors: O’Callaghan Sondra , Yau Hanford
Published: December 2020

Palliation of symptoms related to malignancy-associated hypercalcemia (MAH) is essential and clinically meaningful for patients, given the continued poor prognosis, with high morbidity and mortality associated with this disease process. Historically, agents have been temporizing, having no impact on patient morbidity nor survival. We suggest that cinacalcet can be an efficacious agent to be taken orally, reducing patients’ time in the hospital/clinic settings. It is well-tolerated and maintains serum calcium levels in the normal range, while targeted cancer treatments can be employed. This has a direct, major impact on morbidity. Maintaining eucalcemia can increase quality of life, while allowing targeted therapies time to improve survival. Given that our case (and others) showed calcium reduction in MAH, there is promising evidence that cinacalcet can be more widely employed in this setting. Future consideration should be given to studies addressing the efficacy of cinacalcet in calcium normalization, improvement of quality of life, and impact on survival in patients with MAH. Though the exact mechanism of action for cinacalcet’s reduction in calcium in this setting is not currently known, we can still afford patients the possible benefit from it.
Association of metabolic syndrome components with circulating levels of cytokine clusters in young women
Authors: Tan Xingrong , Hu Wenjing , Yang Shan , Dai Han , Xu Shangcheng , Yang Gangyi , Li Ling , Tang Shiguo , Wang Yi
Published: December 2020

Background

The purpose of this study was to investigate the relationship between circulating zinc α 2-glycoprotein (ZAG), irisin, betatrophin and adiponectin concentrations and metabolic syndrome (MetS) components and to analyze the effects of blood glucose and insulin on these cytokine concentrations in vivo.

Methods

A total of 196 young women, including 78 healthy women and 118 women with MetS components, were recruited for this cross-sectional study. An oral glucose tolerance test and euglycemic-hyperinsulinemic clamp (EHC) were performed in healthy subjects and women with MetS components. An ELISA kit was used to measure serum ZAG, irisin, betatrophin, and adiponectin levels, and their relationship with the MetS components was analyzed.

Results

In women with MetS components, circulating irisin and betatrophin levels were significantly higher than those in the healthy women ((207 (150–248) vs 178 (147–228); P < 0.05) for irisin; (0.51 (0.38–0.63) vs 0.38 (0.23–0.52); P < 0.001) for betatrophin), but circulating ZAG and adiponectin levels were significantly lower (39.8 (26.4–50.4) vs (46.7 (40.6–63.0); P < 0.001) for ZAG; (36.5 (22.0–47.6) vs 41.2 (35.7–54.7); P < 0.01) for adiponectin). FBG, WC, and triglyceride were significantly correlated with the circulating levels of these four cytokines (P < 0.001 or <0.05). All four cytokines were associated with MetS and its components. In response to increasing insulin levels, circulating ZAG concentrations were markedly increased in both healthy subjects and women with MetS components during the EHC. However, serum irisin, betatrophin, and adiponectin levels in both healthy subjects and women with MetS components were significantly reduced compared with baseline.

Conclusion

Serum ZAG, irisin, betatrophin and adiponectin were associated with MetS and might be biomarkers for screening MetS components.
FREQUENCY CHARACTERISTICS OF BRUSHLESS MAGNETOELECTRIC MOTORS OF RETURN-ROTARY MOTION
Authors:   Institute of Electrodynamics of the National Academy of Sciences of Ukraine, pr. Peremohy, 56, Kyiv, 03057, Ukraine,, Akinin K.P. , Kireyev V.G. ,   Institute of Electrodynamics of the National Academy of Sciences of Ukraine, pr. Peremohy, 56, Kyiv, 03057, Ukraine,, Petukhov I.S. ,   Institute of Electrodynamics of the National Academy of Sciences of Ukraine, pr. Peremohy, 56, Kyiv, 03057, Ukraine,, Filomenko A.A. ,   Institute of Electrodynamics of the National Academy of Sciences of Ukraine, pr. Peremohy, 56, Kyiv, 03057, Ukraine,, Mihailik E.M. ,   Institute of Electrodynamics of the National Academy of Sciences of Ukraine, pr. Peremohy, 56, Kyiv, 03057, Ukraine,
Published: December 2020

n this paper, the frequency characteristics of a special brushless magnetoelectric motor of return-rotary motion with sinusoidal and rectangular forms of the carrier signal are investigated. The method of generating a feedback signal on the amplitude of the rotor shaft oscillations angle has been improved by fixing the value of the signal at the moment of reaching its amplitude. The method of calculating the control system of the oscillations angle amplitude is investigated based on the frequency characteristics of the open-loop system by setting the phase stability margin. Examples of the calculation of transient processes of regulation of the oscillations angle amplitude and the effective value of the stator current when starting the motor and changing the mechanical load are given. References 9, figures 6, tables 2.
CONTACTLESS CAPACITIVE SENSOR OF THE SYSTEM FOR MONITORING THE PARAMETERS OF THE BEATING OF THE POWERFUL ELECTRICAL MACHINES SHAFTS
Authors:   Institute of Electrodynamics of the National Academy of Sciences of Ukraine, pr. Peremohy, 56, Kyiv, 03057, Ukraine,, Bereznychenko V.O. , Zaitsev I.O. ,   Institute of Electrodynamics of the National Academy of Sciences of Ukraine, pr. Peremohy, 56, Kyiv, 03057, Ukraine,
Published: December 2020

In this paper presents the results of the definition the need to use a Kelvin guard ring to reduce the impact of external fields and non-uniformity of equipotential lines to change response characteristic of the capacitive sensor with a central high-potential electrode and a Kelvin guard ring. Measuring transducer placing in the immediate vicinity of the electrodes of the sensor, which eliminates the need to use a triaxial cable, was proposed. The sensor is designed to measure powerful generators shafts cylindrical surfaces parameters run-out. Capacitive sensor response characteristic function which depending on distance between the general plane of electrodes of the sensor and the grounded surface of a shaft is determined analytically and by computer simulation methods. The expediency of using computer modeling tools by finite element analysis methods for studying the metrological characteristics of sensors was shown. References 21, figures 4, table 1.
Properties of composite systems based on polymethylsiloxane and silica in the water environment
Authors:   Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine, Krupska T. V. , Gun'ko V. M. ,   Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine, Protsak I. S. ,   Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine, Gerashchenko I. I. ,   Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine, Golovan A. P. ,   Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine, Klymenko N. Yu. ,   Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine, Turov V. V. ,   Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine, Kartel M. T. ,   Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
Published: December 2020

The formation of a composite system based on equal amounts of hydrophobic, porous polymethylsiloxane and hydrophilic nanosilicon A-300 was studied. It is shown that during the formation of a composite system the specific surface of the material is significantly reduced, which is due to the close contact between hydrophobic and hydrophilic particles. When water is added to the composite system, in the process of homogenization under conditions of dosed mechanical loading, the effect of nanocoagulation is manifested – the formation of nanosized particles of hydrated silica inside the polymethylsiloxane matrix, recorded on TEM microphotographs. When measuring the value of the interfacial energy of PMS and PMS/A-300 composite by low-temperature 1H NMR spectroscopy, it was found that the effect of nanocoagulation is manifested in a decrease (compared to the original PMS) energy of water interaction with the surface of the composite obtained under small mechanical conditions. its growth when using high mechanical loads. In the process, the binding of water in heterogeneous systems containing PMS, pyrogenic nanosilica (A-300), water and surfactants – decamethoxine (DMT) was studied. Composite systems were created using metered mechanical loads. It is shown that when filling the interparticle gaps of PMS by the method of hydrosealing, the interphase energy of water in the interparticle gaps of hydrophobic PMS with the same hydration is twice the interfacial energy of water in hydrophilic silica A-300. This is due to the smaller linear dimensions of the interparticle gaps in PMS compared to A-300. In the composite system, A-300/PMS/DMT/H2O there are non-additive growth of binding energy of water, which is probably due to the formation, under the influence of mechanical stress in the presence of water, microheterogeneous areas consisting mainly of hydrophobic and hydrophilic components (microcoagulation). Thus, with the help of mechanical loads, you can control the adsorption properties of composite systems and create new materials with unique adsorption properties.
Fungi-resistant basalt fiber material
Authors:   National Technical University of Ukraine KPI named Igor Sikorsky, Shevchenko V. M. , Guts N. A. ,   National Technical University of Ukraine KPI named Igor Sikorsky, Shpak A. Ye. ,   National Technical University of Ukraine KPI named Igor Sikorsky, Vlasenko N. Ye. ,   National Technical University of Ukraine KPI named Igor Sikorsky, Shulzhenko O. O. ,   National Technical University of Ukraine KPI named Igor Sikorsky
Published: December 2020

Leading industrial countries display the growth of paper industry even more than other industries. The paper is and was very important for our civilization. Technical industries also require the paper. The electro-technical, radio-technical industries use widely various kinds of paper for the production of things like cables, condensers, radio-diffusers, resistors, TV-sets and so on. In the chemical industry papers are used for chromatography, electrophoresis etc. But besides of clear advantages for simple usage there are some another advances for some usage namely materials which are resistant to mold fungi and the microorganisms. It is known that the least subject to the fungi effect are things what are consisted as linen fibers among all as well as sulfite and sulfate cellulose. The artificial fibers are more resistant than natural. Obviously the problem of conservation of already printed on paper materials growths more and more every year. Now it is strange when we can’t read old books which have not being saved in new digital formats. That is why the preserve the old paper literature in worthy state is so important for us and for future generations, and the problem of obtaining so called “biocide” material is so actual. It means such material which may destroy bacteria, mold fungi, and insects. In this work we have used the clays from Zakarpatiyan oblast Horbsky field. These clays are classified as bentonite type and have the following consistence with particular size is between 0,3 and 0,25 mm. The authors studied the sorption of latexes by basalt fibers. The following latexes were investigated: divinyl methyl methacrylate (DMMA), divinyl nitrile (SKN), isoprene nitrile (L-7). A method was used to recharge the surface of cellulose fibers to obtain a flexible, durable filter material. We checked the resistance of materials to mold.