bluuebunny/crossref_metadata_2025 huggingface dataset:
Lived values and modal choices in Sweden: an approximation to potential losses from the low-carbon transition in the transport sector
Authors: Strambo Claudia , Xylia Maria , Gong Jindan
Published: November 2024

To meet the goals set in the Paris Agreement, the transport sector requires transformative changes, not only in terms of prevailing technologies but also with regards to reducing the need for fossil-based transport and increasing the share of public and active transport modes. Policies to enable this transformation are likely to have differentiated impacts on quality of life across society and have the potential to reproduce or deepen existing inequalities. When identifying potential losers from the low-carbon transition, it is important to consider a diverse set of loss categories, including social support networks and attachments a person has to particular people, material things, places and traditions. A key assumption here is that individuals’ perceptions of loss derive from their lived values, i.e., what they consider important in their life. Through a mixed-method approach consisting of a literature review and a survey, this study explores modal choices for realizing activities central to quality of life in Sweden, with a particular focus on societal groups at disadvantage in the transport transition. This article provides new insights on potential losses associated with the low-carbon transition in the transport sector and their distribution across society and reflects on the implications for transitional assistance policy.
Septum of the penis – dissection, anatomical description and functional relevance
Authors: Filipoiu Florin-Mihail , Ion Radu-Tudor , Filipoiu Zoran-Florin , Tulin Adrian-Daniel , Enciu Octavian , Enyedi Mihaly
Published: November 2024

Background

The septum of the penis or the pectiniform septum (from Latina pecten) is a connective structure that separates the two corpora cavernosa of the penis. It is formed through the joining of the circular fibers of the tunica albuginea, which envelops the corpora cavernosa. The septum neither completely separates, nor entirely joins the two corpora cavernosa.

Results

We dissected the penile septum in 10 formalized bodies. The dissections were carried out using magnifying lenses, emphasizing the connective structures. We studied the structure of the septum using transverse and sagittal dissection planes. We identified the penile septum as a structure consisting of clusters of tendinous cords incompletely separating the two cavernous structures. The septum completely separates the two corpora cavernosa in its posterior segment. As we progress forward, the septum starts resembling the tendinous cords that attach to the papillary muscles of the heart. These cords are differentiated from the internal layer of the albuginea of each corpus cavernosum. We evaluated the opportunity of considering the anterior and posterior intercavernous ligaments as septal structures.

Conclusion

In this type of construction, the septum maintains both the hemodynamic and mechanical coherence of the cavernous structures and allows penile movement more efficiently than a continuous septal structure. The septum enables the lengthening of the penis and simultaneous filling with blood of both its corpora cavernosa through the transseptal vascular anastomosis. This allows for penile deformation during erection to be avoided. Our study also provides a description of the way the corpora cavernosa attach to the bulbus of corpus spongiosum.
Cytomegalovirus-associated pancytopenia in a four-month-old infant: a case report
Authors: Gyawali Jeevan , Pudasainee-Kapri Sangita , Agrawal Sumit , Khatri Dhan Bahadur , Adhikari Sugat , Dhunagana Prem Prasad
Published: November 2024

Cytomegalovirus (CMV) is a beta-herpes virus causing common infections, often asymptomatic in healthy individuals. However, it poses increased risks to immunocompromised individuals and can cause congenital infections, leading to severe disabilities. CMV infection can cause significant hematological abnormality. A four-month-old female infant was admitted for decreased feeding for two days. She was severely pale, without hepatosplenomegaly. In initial laboratory investigations hemoglobin, platelet count, and white blood cells were decreased. The patient was transfused with whole blood and referred to a tertiary care center. Further workup, including bone marrow biopsy, showed hypocellular marrow. The Urine CMV Polymerase Chain Reaction (PCR) test returned highly positive with a viral load of 1,700,000 copies/mL. This patient was diagnosed with CMV-associated bone marrow suppression, and she was prescribed valganciclovir at a dosage of 16 mg/kg/dose every 12 h for 6 months. She had shown significant hematologic parameter improvement during subsequent follow-up. Pancytopenia in infancy should include a differential diagnosis for CMV infection. The early recognition and correct infection management, including antiviral therapy and symptomatic treatment, yield a better prognosis.
HIV Social-network intervention more effective in older populations in Kenya
Authors: Zoughbie Daniel E. , Huddleston Dillon , Watson Kathleen , Ding Eric L.
Published: November 2024

US President’s Emergency Plan for HIV/AIDS has been credited with saving 25 million lives in sub-Sahara Africa and, as such, constitutes a preeminent US foreign policy achievement of the twenty-first century. However, the implementation of effective HIV/AIDS pharmacological interventions remains a challenge in rural Kenyan communities. Of particular importance are patient retention and care engagement and their interaction with age disparities that are sensitive to different socioeconomic contexts, as well as time-in-treatment. For the first time, we perform an intermediation and triple interaction intent-to-treat secondary analysis on a social network-based randomized controlled trial. We hypothesize that the temporal interactions of critical demographic features with a treatment/control indicator variable may significantly explain patient retention and that these results are intermediated by social network phenomena. We find that not only does extended time-in-treatment significantly improve primary outcomes, but the threefold interaction along with age and treatment itself is sufficiently flexible to fit the data remarkably well without unnecessary elaboration, an effect that is mediated via internalized stigma. This strongly suggests that patient retention varies by age group. Rather than deploying one-size-fits-all solutions, foreign and public policymakers should invest in research that considers how interventions might be optimized for different ages.

Trial registration Clinical Trial Number. NCT02474992 (note: the main trial report was published here https://doi.org/10.1371/journal.pone.0255945.) Date of submission: June 6, 2015.
Association of lifetime lactation and characteristics of menopause: a longitudinal cohort study
Authors: Scime Natalie V. , Huang Beili , Brockway Meredith Merilee , Brown Hilary K. , Brennand Erin A.
Published: November 2024

Background

Lactation has many established benefits for women’s long-term health; however, its influence on menopause is less clear. This study investigated the association between lifetime duration of lactation and the timing and type of menopause in midlife women.

Methods

We analyzed survey data on 19,783 parous women aged 40 to 65 years at enrollment in the Alberta’s Tomorrow Project (2000–2022), a prospective community-based cohort study in Alberta, Canada. Duration of lifetime lactation across all births was categorized as: <1 month (reference group; 19.8% of women), 1–3 months (12.1%), 4–6 months (11.7%), 7–12 months (18.8%), and ≥ 13 months (37.7%). Women were classified as premenopause, natural menopause (age at 1 year after the final menstrual period), surgical menopause (age at bilateral oophorectomy), or indeterminate menopause (age at premenopausal hysterectomy with ovarian preservation). Flexible parametric survival analysis and multinomial logistic regression were used to analyze menopause timing and type, respectively, according to lactation status and controlling for birth year, education, parity, hormonal contraceptive use, and smoking.

Results

In a dose-response manner, longer lactation was associated with reduced risk of natural menopause before age 50 (for ≥ 13 months of lactation, adjusted hazard ratio at age 45: 0.68, 95% CI 0.59–0.78), surgical menopause before age 55 (age 45: 0.56, 0.50–0.63), and indeterminate menopause before age 50 (age 45: 0.75, 0.69–0.82). Longer lactation was associated with lower odds of surgical (adjusted odds ratio 0.54, 95% CI 0.45–0.66) and indeterminate menopause (0.63, 0.55–0.73), compared to natural menopause.

Conclusions

Optimizing the timing of natural menopause and reducing risks of early surgical and indeterminate menopause may be novel maternal benefits of breastfeeding.
(Dis)connected by design? Using participatory citizen science to uncover environmental determinants of social connectedness for youth in under-resourced neighbourhoods
Authors: Sones Meridith , Holden Meg , Kestens Yan , King Abby C. , Rennie Mimi , Winters Meghan
Published: November 2024

Background

Social isolation and loneliness are a growing public health concern. Inadequacies in neighbourhood social infrastructure can undermine social connectedness, particularly for youth, who are dependent on their local environments yet often marginalized from public spaces and city planning. Integrating citizen science with participatory action research, the Youth.hood study set out to explore how neighbourhood built environments help or hinder social connectedness from the understudied perspective of youth in under-resourced and racialized communities.

Methods

Youth (n = 42) from three neighbourhoods in Vancouver, Canada were recruited to: (1) Assess environmental assets and barriers to connectedness in their neighbourhoods using a digital photovoice app; (2) Analyze and prioritize their collective data into themes; and (3) Design and advocate for environmental improvements through a participatory workshop and forum with residents, city planners, and elected officials. Data on participant characteristics and neighbourhood perceptions were collected via an online survey and analyzed descriptively. Participatory analysis was conducted with youth using methods from thematic analysis, photovoice, and design thinking.

Results

Youth captured 227 environmental features impacting their connectedness. The most frequently reported assets were parks and nature (n = 39, 17%), including formal and informal green spaces, and food outlets (n = 25, 11%). Top barriers included poor neighbourhood aesthetics (n = 14, 6%) and inadequate streets and sidewalks (n = 14, 6%). Thematic analysis with youth underscored four themes: (1) Connecting through mobility: The fun and functionality of getting around without a car; (2) The power of aesthetics: Mediating connections to people and place; (3) Retreating to connect: Seeking out social and restorative spaces for all; and (4) Under-resourced, not under-valued: Uncovering assets for sociocultural connection. Youth described their local environments as affording (or denying) opportunities for physical, emotional, and cultural connection at both an individual and community level.

Conclusion

Our findings extend evidence on key environmental determinants of social connectedness for youth, while highlighting the potential of community design to support multiple dimensions of healthy social development. Additionally, this work demonstrates the resilience and agency of youth in under-resourced settings, and underscores the importance of honouring assets, co-production, and intergenerational planning when working to advance healthy, connected, and youthful cities.
What are the priorities of consumers and carers regarding measurement for evaluation in mental healthcare? Results from a Q-methodology study
Authors: O’Loughlin Rachel , Lambert Caroline , Olsen Gemma , Thwaites Kate , Saltmarsh Keir , Anderson Julie , Devlin Nancy , Hiscock Harriet , Dalziel Kim
Published: November 2024

Background

The purpose of this study was to identify and describe common views of people with lived experience of mental health challenges – consumers and carers, families and supporters – of what they consider the most important measures to include in health economic evaluations which assess the incremental value of competing options in mental health care.

Methods

Participants (n = 111) were people living in the state of Victoria, Australia, who identified as consumers of mental healthcare (n = 38); carers, family members and/or supporters (n = 43); or both (n = 30). Factor analysis based on Q-Methodology was used to identify clusters of people who hold similar viewpoints. Common viewpoints were described in terms of the characteristics of the group, and a qualitative interpretation was conducted on the basis of distinguishing statements and quotes provided in participants’ own words.

Results

We identified four common views: (1) safety before all else, prioritizing physical, sexual and psychological safety; (2) hope and partnership in processes of care; (3) physical and emotional health and wellbeing; and (4) care access, continuity and partnership with families. Although different priorities were identified for each viewpoint, key priority areas that were common to all views were having an environment in the health service that fosters respect and dignity, and that consumers feel heard and listened to. In sub-group and qualitative analyses, differences were observed regarding the likelihood of consumers and carers holding each of the views, as well as by age group.

Conclusions

While some differences were noted between the views of consumers and carers and different age groups, there was also common ground regarding what outcomes are of most importance to measure. Including these measures in evaluation frameworks would provide a way of focussing mental healthcare decisions on the aspects of mental healthcare that are of most value to consumers and carers, thereby addressing an important shortcoming of current approaches to decision-making in mental healthcare.
Glucagon-like peptide-1 receptor agonists (GLP-1RAs) as treatment for nicotine cessation in psychiatric populations: a systematic review
Authors: Lee Serene , Li Maggie , Le Gia Han , Teopiz Kayla M. , Vinberg Maj , Ho Roger , Au Hezekiah C. T. , Wong Sabrina , Valentino Kyle , Kwan Angela T. H. , Rosenblat Joshua D. , McIntyre Roger S.
Published: November 2024

Background

Nicotine use and nicotine use disorder (NUD) are the leading causes of preventable death in the United States. Persons with mental disorders  (e.g., bipolar disorder) are differentially susceptible to nicotine use. Glucagon-like peptide-1 receptor agonists (GLP-1RAs) are indicated for type 2 diabetes mellitus (T2DM) and obesity and show preliminary evidence of efficacy in addiction-related behaviours. Herein, we synthesize extant preclinical and clinical evidence evaluating the effect of GLP-1RAs on neurobiological systems and behaviours salient to nicotine consumption and cessation.

Methods

Online databases (MedLine, Embase, AMED, PsychINFO, JBI EBP Database, PubMed, Web of Science, Google Scholar) were searched from inception to May 21, 2024. Relevant studies were also extracted from the reference lists of the obtained articles. All articles were screened against inclusion and exclusion criteria.

Results

Administration of GLP-1RAs reduced nicotine self-administration and nicotine-seeking behaviour in animal models that, in some cases, is sustained beyond exposure to the agent. GLP-1RAs also mitigated post-nicotine cessation weight gain, craving, withdrawal, and hyperphagia. The preceding effects are attributable to modulation of reward-related brain regions (e.g., mesolimbic dopamine system), resulting in nicotine aversion. GLP-1RAs were also efficacious as adjunctive therapies [e.g., in combination with nicotine replacement therapies (NRTs)].

Conclusion

The multi-effect characteristics in NUD paradigms provide a compelling rationale for large, adequately powered, long-term, randomized controlled trials of GLP-1RAs in the treatment and prevention of NUD. The replicated effect on mitigating post-nicotine cessation weight gain is a differentiating feature of GLP-1RAs from extant proven therapies for NUD.
Advancing hyperspectral imaging techniques for root systems: a new pipeline for macro- and microscale image acquisition and classification
Authors: Faehn Corine , Konert Grzegorz , Keinänen Markku , Karppinen Katja , Krause Kirsten
Published: November 2024

Background

Understanding the environmental impacts on root growth and root health is essential for effective agricultural and environmental management. Hyperspectral imaging (HSI) technology provides a non-destructive method for detailed analysis and monitoring of plant tissues and organ development, but unfortunately examples for its application to root systems and the root-soil interface are very scarce. There is also a notable lack of standardized guidelines for image acquisition and data analysis pipelines.

Methods

This study investigated HSI techniques for analyzing rhizobox-grown root systems across various imaging configurations, from the macro- to micro-scale, using the imec VNIR SNAPSCAN camera. Focusing on three graminoid species with different root architectures allowed us to evaluate the influence of key image acquisition parameters and data processing techniques on the differentiation of root, soil, and root-soil interface/rhizosheath spectral signatures. We compared two image classification methods, Spectral Angle Mapper (SAM) and K-Means clustering, and two machine learning approaches, Random Forest (RF) and Support Vector Machine (SVM), to assess their efficiency in automating root system image classification.

Results

Our study demonstrated that training a RF model using SAM classifications, coupled with wavelength reduction using the second derivative spectra with Savitzky-Golay (SG) smoothing, provided reliable classification between root, soil, and the root-soil interface, achieving 88–91% accuracy across all configurations and scales. Although the root-soil interface was not clearly resolved, it helped to improve the distinction between root and soil classes. This approach effectively highlighted spectral differences resulting from the different configurations, image acquisition settings, and among the three species. Utilizing this classification method can facilitate the monitoring of root biomass and future work investigating root adaptations to harsh environmental conditions.

Conclusions

Our study addressed the key challenges in HSI acquisition and data processing for root system analysis and lays the groundwork for further exploration of VNIR HSI application across various scales of root system studies. This work provides a full data analysis pipeline that can be utilized as an online Python-based tool for the semi-automated analysis of root-soil HSI data.
Parental obesity, health determinants, and cardiometabolic risk according to sleep duration in schoolchildren: analysis through structural equations
Authors: Brand Caroline , Batista Lemes Vanilson , Paula Sehn Ana , Agostinis-Sobrinho Cesar , Henriquez-Maquehue Fernanda , Jofré-Saldía Emilio , Ibacache-Saavedra Paulina , Farias-Valenzuela Claudio , Villa-González Emilio , Priscila Reuter Cézane
Published: November 2024

Background

the independent association between parental obesity, sleep and lifestyle habits with cardiometabolic risk factors in children and adolescents has been widely explored in the literature. Our study represents a novel approach to comprehensively investigate a complex model encompassing various aspects associated with cardiometabolic risk in youth. Thus, the aim of the present study was to verify the relationship between parental obesity and cardiometabolic risk in children and adolescents, considering the mediator role of health indicators according to sleep time.

Methods

This cross-sectional study was conducted on 3,973 children and adolescents aged 6 to 17 years attending public and private schools in a city located in Southern Brazil. Sleep duration, lifestyle, physical activity, natural food consumption, and parental obesity were evaluated through self-reported questionnaires. Physical fitness was evaluated according to the protocols of Projeto Esporte Brasil. The body fat percentage was evaluated through the measures of tricipital and subscapular folds, and the body mass index was calculated. The clustered metabolic risk score (cMetS) considered the summing z-scores of waist circumference, systolic blood pressure, triglycerides, total cholesterol/high-density lipoprotein cholesterol ratio, and fasting glucose, divided by five. A structural equation model was applied for statistical analysis. Results: The relationship between parental obesity and cardiometabolic risk was observed in children and adolescents with adequate sleep, being significant (p < 0.05) mediated by physical activity, natural food, physical fitness, and lifestyle. This was also observed when parental education was included in the inadequate sleep cluster model. Conclusion: Present findings underscore the importance of sufficient sleep duration as a critical factor in understanding the complex interplay between parental obesity and cardiometabolic risk in children and adolescents.
Comparative performance analysis of different microfilaria testing methods for Dirofilaria immitis in canine blood
Authors: Smith Rachel C. , Tomlinson Trey D. , Bowles Joy V. , Starkey Lindsay A.
Published: November 2024

Background

Microfilaria (MF) testing is an essential part of canine heartworm diagnostics, and it is recommended by the American Heartworm Society that a MF test be performed in tandem with antigen testing on every dog, every year, regardless of prevention status or history. There are a variety of methods that can be used to detect MF in canine whole blood; however, these methods widely vary in their sensitivities as well as practical factors, including time investment and cost. Additionally, some MF tests offer the advantage of being quantitative or allowing for morphological or molecular species identification, while other tests should only be used qualitatively.

Methods

The purpose of this study is to evaluate the quantitative and qualitative performance of MF tests, including the 20 μL count, wet mount, 9 μL and 40 μL hematocrit tubes, thin smear, thick smear, modified Knott test (MKT), and conventional polymerase chain reaction (PCR).

Results

Qualitatively, there was little difference in the performance of the 20 μL count, wet mount, MKT, and PCR. The MKT and PCR are the optimal MF tests, as these perform most reliably for detecting positives even when the MF per milliliter is relatively low, and in most cases, these two methods also allow for species-level confirmation of the identity. However, PCR tends to be a very costly test, and both PCR and MKT require a greater degree of expertise and time investment to perform than other tests. Even the lowest performance tests, including the thin smear and hematocrit tube methods, can reliably detect MF at very high burdens; although, caution should be advised when using low reliability methods, since there is a greater likelihood of failing to identify MF-positive dogs.

Conclusions

Microfilaria (MF) testing is an essential part of heartworm diagnosis and screening in dogs, and test selection should balance practical factors such as cost and time investment with the patient’s risk of infection based on prevention status and history, clinical signs, and antigen testing results. This approach to MF testing will help minimize cost while avoiding failure to detect MF in infected dogs, especially when MF burden is low.

Graphical Abstract
Mesenchymal stem cells: a novel therapeutic approach for feline inflammatory bowel disease
Authors: Xie Qiyun , Gong Saisai , Cao Jintao , Li Aoyun , Kulyar Md. F. , Wang Bingyun , Li Jiakui
Published: November 2024

Background

Inflammatory bowel disease (IBD) poses a significant and growing global health challenge, affecting both humans and domestic cats. Research on feline IBD has not kept pace with its widespread prevalence in human populations. This study aimed to develop a model of feline IBD by incorporating dextran sulfate sodium (DSS) to evaluate the therapeutic potential of MSCs and to elucidate the mechanisms that enhance their action.

Methods

We conducted a comprehensive clinical assessment, including magnetic resonance imaging (MRI), endoscopy, and histopathological examination. Additionally, alterations in intestinal microbiota were characterized by 16 S rDNA sequencing, and the influence of MSCs on IBD-related gene expression was investigated through transcriptome analysis.

Results

According to our findings, MSC treatment significantly mitigated DSS-induced clinical manifestations, reduced inflammatory cell infiltration, decreased the production of inflammatory mediators, and promoted mucosal repair. Regarding the intestinal microbiota, MSC intervention effectively corrected the DSS-induced dysbiosis, increasing the presence of beneficial bacteria and suppressing the proliferation of harmful bacteria. Transcriptome analysis revealed the ability of MSCs to modulate various inflammatory and immune-related signaling pathways, including cytokine-cytokine receptor interactions, TLR signaling pathways, and NF-κB pathways.

Conclusion

The collective findings indicate that MSCs exert multifaceted therapeutic effects on IBD, including the regulation of intestinal microbiota balance, suppression of inflammatory responses, enhancement of intestinal barrier repair, and modulation of immune responses. These insights provide a solid scientific foundation for employing MSCs as an innovative therapeutic strategy for IBD and pave the way for future clinical explorations.
Sex differences in the human brain related to visual motion perception
Authors: Liu Dong-Yu , Li Ming , Yu Juan , Gao Yuan , Zhang Xiaotong , Hu Dewen , Northoff Georg , Song Xue Mei , Zhu Junming
Published: November 2024

Background

Previous studies have found that the temporal duration required for males to perceive visual motion direction is significantly shorter than that for females. However, the neural correlates of such shortened duration perception remain yet unclear. Given that motion perception is primarily associated with the neural activity of the middle temporal visual complex (MT+), we here test the novel hypothesis that the neural mechanism of these behavioral sex differences is mainly related to the MT+ region.

Methods

We utilized ultra-high field (UHF) MRI to investigate sex differences in the MT+ brain region. A total of 95 subjects (48 females) participated in two separate studies. Cohort 1, consisting of 33 subjects (16 females), completed task-fMRI (drafting grating stimuli) experiment. Cohort 2, comprising 62 subjects (32 females), engaged in a psychophysical experiment measuring motion perception along different temporal thresholds as well as conducting structural and functional MRI scanning of MT+.

Results

Our findings show pronounced sex differences in major brain parameters within the left MT+ (but not the right MT+, i.e., laterality). In particular, males demonstrate (i) larger gray matter volume (GMV) and higher brain’s spontaneous activity at the fastest infra-slow frequency band in the left MT+; and (ii) stronger functional connectivity between the left MT+ and the left centromedial amygdala (CM). Meanwhile, both female and male participants exhibited comparable correlations between motion perception ability and the multimodal imaging indexes of the MT+ region, i.e., larger GMV, higher brain’s spontaneous activity, and faster motion discrimination.

Conclusions

Our findings reveal sex differences of imaging indicators of structure and function in the MT+ region, which also relate to the temporal threshold of motion discrimination. Overall, these results show how behavioral sex differences in visual motion perception are generated, and advocate considering sex as a crucial biological variable in both human brain and behavioral research.
The introduction of a highly virulent PRRSV strain in pig farms is associated with a change in the pattern of influenza A virus infection in nurseries
Authors: Domingo-Carreño Ivan , Serena Maria Soledad , Martín-Valls Gerard Eduard , Clilverd Hepzibar , Aguirre Laia , Cortey Martí , Mateu Enric
Published: November 2024

The present study aimed to determine the dynamics of influenza A virus (IAV) infection in two endemically infected farms (F1 and F2), where a longitudinal follow-up of piglets was performed from birth to 8–12 weeks of age. During the study, a highly virulent isolate of porcine reproductive and respiratory syndrome virus (PRRSV) was introduced on both farms. This allowed us to examine the impact of such introduction on the patterns of infection, disease, and the antibody response of pigs to IAV infection. The introduction of the new PRRSV strain coincided with a change in the dynamics of IAV infection on both farms. In F1, the cumulative incidence of IAV increased from 20% before the outbreak to 67.5%, together with the existence of animals that tested positive for IAV (RT‒qPCR) in nasal swabs for two or more consecutive samples. In F2, the cumulative incidence of IAV increased from 50% before the PRRSV outbreak to 70%, and the proportion of prolonged IAV shedders increased sharply. Additionally, some animals were infected with the same IAV twice during the observation period. In contrast to previous reports, our study revealed that prolonged shedding was not related to the titres of maternally derived antibodies at the time of infection but was significantly (p < 0.05) related to PRRSV infection status. Notably, both before and after the PRRSV outbreak, a high proportion of IAV-infected piglets did not seroconvert, which was significantly (p < 0.05) related to the hemagglutination inhibition titres against IAV when infected.
Potassium channels in depression: emerging roles and potential targets
Authors: Zhang Jiahao , Zhu Yao , Zhang Meng , Yan Jinglan , Zheng Yuanjia , Yao Lin , Li Ziwei , Shao Zihan , Chen Yongjun
Published: November 2024

Potassium ion channels play a fundamental role in regulating cell membrane repolarization, modulating the frequency and shape of action potentials, and maintaining the resting membrane potential. A growing number of studies have indicated that dysfunction in potassium channels associates with the pathogenesis and treatment of depression. However, the involvement of potassium channels in the onset and treatment of depression has not been thoroughly summarized. In this review, we performed a comprehensive analysis of the association between multiple potassium channels and their roles in depression, and compiles the SNP loci of potassium channels associated with depression, as well as antidepressant drugs that target these channels. We discussed the pivotal role of potassium channels in the treatment of depression, provide valuable insights into new therapeutic targets for antidepressant treatment and critical clues to future drug discovery.
Israeli dentists’ knowledge, attitudes, and practices regarding smoking cessation care
Authors: Vishnevsky Tamar , Aperman-Itzhak Tal , Tayeb Itzhak , Bar-Zeev Yael
Published: November 2024

Background

The 5A’s model for brief smoking cessation care (SCC) is recommended for dentists to reduce the negative impacts of smoking on oral health. This study investigates Israeli dentists' adherence to the 5A’s guidelines and explores factors influencing their knowledge, attitudes, and practices.

Methods

An online cross-sectional survey was conducted among Israeli dentists during June–August 2020. The questionnaire included sociodemographic, smoking, and professional characteristics; knowledge (10 true/false statements); attitudes regarding SCC [based on the Theoretical Domains Framework (TDF)] using 13 statements (1–5 Likert scale), for a composite mean attitude score; and 5A’s performance (1–5 Likert scale, never to always). Two primary outcomes were analysed: (1) performing all of the 5A’s ‘often or always’; and (2) performing ‘always’ the first two steps (“Ask” and “Advise”). Multivariable logistic regression explored the association between the various characteristics and the primary outcomes.

Results

Overall, n = 410 responded. Mean knowledge score was 2.58 (SD = 1.51). Mean attitude score was 2.65 (SD = 0.60). Performance of all 5A’s was low with 14.1% (n = 57) reporting completing all 5A’s ‘often or always’, while 34.1% (n = 139) reported ‘always’ performing ‘Ask’ and ‘Advise’. Specialists had better odds of ‘often or always’ performing the 5A’s (adjusted OR = 2.01, p = .022) and ‘always’ performing ‘Ask and Advise’ (adjusted OR = 1.71, p = .022).

Conclusions

This study highlights the insufficient performance of SCC among Israeli dentists, revealing gaps in knowledge and attitudes related to SCC. Various measures, such as training, automatic referral systems, and integrating SCC as quality measures, may improve SCC provision among Israeli dentists.
Involvement of family caregivers in dementia care research: a scoping review protocol
Authors: Jagoda Franziska Anushi , Hirt Julian , Mueller Claudia , Halek Margareta
Published: November 2024

Background

Family caregivers of people with dementia are a distinct group due to the particularly stressful and time-intensive care situation at home. Despite these challenges, involving them in research is crucial to better understand and address their specific needs. However, little evidence exists regarding a tailored approach for researchers for this group considering their situation at home.

Methods

A scoping review will be conducted following the Joanna Briggs Institute methodological guidance, including the databases MEDLINE (PubMed), CINAHL, Scopus (Elsevier), and PsycINFO (EBSCO). The review will include family caregivers of people with dementia, regardless of age, gender, or ethnicity, who have been actively involved in research throughout the research process. Moreover, sources of evidence from any country in both English and German are eligible for inclusion. Sources will be screened by two independent reviewers. Results will be extracted using a tailored charting tool and presented in the final report according to the research questions and objectives.

Discussion

Developing a tailored approach to involve family caregivers of people with dementia in research and development has profound importance to both the scientific community and the target group itself.

Systematic review registration

Open Science Framework https://doi.org/10.17605/OSF.IO/PMZYV.
Understanding soil and ecosystem respiration in a dune-meadow cascade ecosystem
Authors: Kang Xueer , Liu Tingxi , Hao Lina , Duan Limin , Wu Rong , Tong Xin , Bao Yongzhi , Wang Yixuan , Gong Yu , Cao Wenmei
Published: November 2024

Arid and semi-arid regions, which account for more than 30% of the Earth's land area, increasingly dominate the spatiotemporal trends in global carbon fluxes. The Horqin Sandy Land is a typical semi-arid fragile ecosystem in northern China. Understanding the components of the carbon budget in ecosystems under conditions of extreme soil moisture limitations provides a foundation for comprehending the carbon balance in semi-arid ecosystems. The seasonal and diurnal variations in soil respiration (Rs) in semi-mobile dune (SD) and meadow wetland (MW) ecosystems of the Horqin Sandy Land were examined, and the sources of CO2 emissions from Rs were identified using stable carbon isotopes. The responses of Rs and ecosystem respiration (Reco) to environmental temperature, moisture and leaf area index (LAI) were revealed. The results showed that on a seasonal scale, in SD with soil moisture content (Ms) below field capacity (FC), Ms had a greater influence on Rs than soil temperature (Ts) during the growing season. Changes in the LAI during the middle and late growth period affected Rs by altering root carbon supply. In MW, the most favorable Ms for Rs was near FC. The increase in LAI before mowing could effectively promote root and soil microbial respiration, and the decomposition of litter driven by Ts was the main form of Rs at this time. After mowing, root respiration and soil microbial respiration were the main processes contributing to CO2 emissions. On a daily scale, relative humidity (RH) dominated the Rs variation under dry conditions, whereas in other conditions, the Rs was adequately explained by temperature in SD and MW. The overall Reco was larger than Rs, but occasionally Rs was greater than Reco. The effects of temperature, moisture and LAI on Reco and Rs varied with growing season. Adding factors, such as ecosystem type, vegetation growth, water, and heat, to the carbon cycle model can improve predictions of carbon emissions, and aid in further management decisions in arid and semi-arid areas.
Seed market dynamics and diffusion of new wheat varieties in Bihar, India: a supply-side perspective
Authors: Kala-Satheesh Hari Krishnan , Kuriyedath Drisya , Jaleel Jesna , Rahman E. P. Nihal , Sathyan Archana Raghavan , Khed Vijayalaxmi D. , Cariappa A. G. Adeeth , Krishna Vijesh V.
Published: November 2024

An examination of the dynamics of seed markets in Bihar, India, reveals a paradox—despite an influx of wheat varieties bred by public and private sectors and the proliferation of seed market networks in rural villages, older wheat varieties remain prevalent—necessitating a thorough investigation of the seed distribution system. Unlike most empirical studies that examine the adoption of new and improved crop varieties from a farmer's perspective, our study shifts the focus to the seed supply side. We analyse data collected from 200 private seed dealers who cater to the needs of over 163,000 farmers spread across 10 districts in Bihar. We use descriptive statistics alongside dealer-level and varietal-level regression models to examine the relationship between seed sales and varietal age. Findings indicate that the number of varieties available with a dealer (varietal richness) is positively associated with the number of seed buyers (dealer’s reach) and the total quantity of seeds sold. Private varieties are in demand despite their higher prices. Dealer-level models showed that varietal age affects neither the reach nor the sales, allowing older public-sector varieties to coexist with more recent private-sector ones. However, the varietal-level regression models show that dealers rank the new varieties higher as the ones being sold more. To explore the potential of private seed markets to reduce the proliferation of old wheat varieties that are more susceptible to evolving biotic and abiotic stress factors, we recommend strengthening the varietal registration and seed certification processes, implementing better seed traceability systems, and fostering public–private partnerships in variety development and seed dissemination. Investing in market experiments to incentivize seed dealers to engage in quality assurance can help refine strategies and ensure efficient and inclusive dissemination of promising wheat varieties.
Dietary supplementation with citrus peel extract in transition period improves rumen microbial composition and ameliorates energy metabolism and lactation performance of dairy cows
Authors: Ju Lingxue , Shao Qi , Fang Zhiyuan , Trevisi Erminio , Chen Meng , Song Yuxiang , Gao Wenwen , Lei Lin , Li Xinwei , Liu Guowen , Du Xiliang
Published: November 2024

Background

During the transition period, excessive negative energy balance (NEB) lead to metabolic disorders and reduced milk yield. Rumen microbes are responsible for resolving plant material and producing volatile fatty acids (VFA), which are the primary energy source for cows. In this study, we aimed to investigate the effect of citrus peel extract (CPE) supplementation on rumen microbiota composition, energy metabolism and milk performance of peripartum dairy cows.

Methods

Dairy cows were fed either a basal diet (CON group) or the same basal diet supplemented with CPE via intragastric administration (4 g/d, CPE group) for 6 weeks (3 weeks before and 3 weeks after calving; n = 15 per group). Samples of serum, milk, rumen fluid, adipose tissue, and liver were collected to assess the effects of CPE on rumen microbiota composition, rumen fermentation parameters, milk performance, and energy metabolic status of dairy cows.

Results

CPE supplementation led to an increase in milk yield, milk protein and lactose contents, and serum glucose levels, while reduced serum concentrations of non-esterified fatty acid, β-hydroxybutyric acid, insulin, aspartate aminotransferase, alanine aminotransferase, and haptoglobin during the first month of lactation. CPE supplementation also increased the content of ruminal VFA. Compared to the CON group, the abundance of Prevotellaceae, Methanobacteriaceae, Bacteroidales_RF16_group, and Selenomonadaceae was found increased, while the abundance of Oscillospiraceae, F082, Ruminococcaceae, Christensenellaceae, Muribaculaceae UCG-011, Saccharimonadaceae, Hungateiclostridiaceae, and Spirochaetaceae in the CPE group was found decreased. In adipose tissue, CPE supplementation decreased lipolysis, and inflammatory response, while increased insulin sensitivity. In the liver, CPE supplementation decreased lipid accumulation, increased insulin sensitivity, and upregulated expression of genes involved in gluconeogenesis.

Conclusions

Our findings suggest that CPE supplementation during the peripartum period altered rumen microbiota composition and increased ruminal VFA contents, which further improved NEB and lactation performance, alleviated lipolysis and inflammatory response in adipose tissue, reduced lipid accumulation and promoted gluconeogenesis in liver. Thus, CPE might contribute to improve energy metabolism and consequently lactation performance of dairy cows during the transition period.
Spatial heterogeneity analysis on distribution patterns and underlying determinants of historical rural settlements: a case study in Taihang Baxing
Authors: Yuan Sinan , Mu Nanshuo , Bai Jinni , Zhang Han
Published: November 2024

As vital carriers of cultural heritage, rural areas are facing the threats of degradation and urbanization during rapid urbanization processes. In recent years, China has formulated and implemented a series of national policies, regulations, and heritage protection lists to safeguard and perpetuate the traditional patterns and historical features of rural heritage. However, these rural heritage listings exhibit significant macro-level distributional imbalances and generally lack consideration of regional characteristics. This study uses the Taihang Baxing (THBX) in China as a case study, initially employing historical local documents and maps as base data. It utilizes the stability in the evolution of Chinese characters to compare historical and contemporary village names, filtering out existing historical rural settlements (HRS) to construct a dataset. Further, a multi-scale geographically weighted regression (MGWR) model is applied to analysis the impacts of natural, economic, and social factors on the concentration of HRS (CHRS). The study reveals that the CHRS in the region is markedly characterized by clustering, with evident spatial heterogeneity in the impacts of various factors. Slope (SLP), total annual precipitation (TP), and potential crop yield (PCY) significantly influence the CHRS globally, while elevation (ELV), distance to ancient path (DAP) and surface runoff (DSR), gross domestic product (GDP), and road density (RD) have significant local impacts. This research offers a methodological framework for HRS data compilation and provides theoretical and methodological references for national and local levels of rural heritage protection, optimization of rural spatial patterns, prediction of rural evolution pathways, and implementation of China’s rural revitalization strategy.
Simulating urban evolution by H-GIS and H-BIM: a temporal and spatial study of Pingjiang map
Authors: Mu Pania Yanjie , Fraser Sarah E. , Peng Maolin
Published: November 2024

Historical maps provide insights into the morphology, cultural, and political shifts of ancient societies. Pingjiang Map is one of the earliest comprehensive ancient Chinese maps, depicting the heritage water city of Suzhou. This study takes it as the subject to examine the temporal and spatial reconstructions of Suzhou city after its devastation in the Song-Jin wars during the twelfth–thirteenth centuries. It uses historical geographical information system (H-GIS) and historical building information management (H-BIM) to simulate Suzhou city in the Southern Song dynasty (1127–1279) and the city’s subsequent modernization to the present. By comparing and contrasting Pingjiang Map, the Song dynasty Suzhou, and the current city in the configuration of clusters such as urban wall, canal, street, government and citadel, temple, and residential ward, we establish evolutionary links between Pingjiang Map and the city, providing insights to the mechanism underlying urban transformation. Through H-BIM, we conducted a temporal and spatial analysis of the twelfth–thirteenth centuries’ citadel of Suzhou, simulating the construction sequence of palatial artefacts. Our research argues that Pingjiang Map stele forged an imperial vision, serving both for documentation and planning. It encoded building information for the central government to manage post-war reconstruction. The research establishes the workflow of ‘SQS’—spatialization, quantitative analysis, and simulation—to investigate the evolution of imperial cities and their cartography.
Development of a new device for CO2 microdiffusion analysis of fluid samples from volcanic areas without using a fixative
Authors: Yaguchi Muga , Ohba Takeshi
Published: November 2024

We propose a new reaction device for the microdiffusion analysis of CO2 in fluid samples from volcanic areas to improve the conventional analysis procedure, which requires a fixative and good handling skills. To accomplish this, a small resin reaction cell for the sample solution and acid (initiator for CO2 liberation) was devised and placed in a commercially available air-tight glass container with a Petri dish to form a microdiffusion analysis unit. The analysis was based on the principles of the conventional manual titration method that uses Ba(OH)2 as the CO2 absorbent. Using the new device, 99.5% recovery, with a relative standard deviation of 4.5% (n = 8), was achieved in a test analysis for 0.03 mmol of CO2 liberated from a Na2CO3 solution as a simulated sample. The analytical performance of the new device was almost comparable to that of conventional units under the experimental conditions of this study. Applying this new device for analyzing fluid samples from volcanic areas will contribute to enhancing the performance by reducing the possibility of CO2 contamination and by simplifying the process by eliminating the need for preparatory cleaning for fixative application.

Graphical Abstract