bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Wide Identification of Heat Shock Transcription Factor Family and Key Members Response Analysis to Heat Stress in Loquat
Authors: Deng Chaojun , Chen Yongping , Wei Weilin , Chen Xiuping , Jiang Jimou
Published: November 2024

Eriobotrya japonica (loquat) is an evergreen fruit tree of the apple tribe in Rosaceae with high edible and medicinal value. The yield and quality of loquat fruits are significantly influenced by environmental stress, particularly heat stress during fruit ripening. In this study, thirty EjHSFs were identified in the loquat genome. Twenty-nine EjHSFs were unevenly distributed across sixteen chromosomes, except Chr-6. A synteny analysis revealed that twenty-six EjHSF genes had undergone duplication events. Twenty-nine EjHSF genes were found to be in sync with HSF in apples while also diverging with other Rosaceae species. A phylogenetic analysis revealed that EjHSFs could be divided into three categories, including eighteen EjHSF-A, ten EjHSF-B, and two EjHSF-C. Twenty-nine members of the EjHSF family showed high homology to those of Malus domestica and Gillenia trifoliate. A promoter analysis retrieved thirty-three CAREs that were functionally relevant and connected to the expression of HSFs. Among these, the promoters of twenty-three EjHSF genes possessed at least one STRE element that could be activated by heat shock. Eleven of these EjHSFs were highly expressed in loquat tissues, namely EjHSF-B4a, EjHSF-A4a, EjHSF-A4d, and EjHSF-C1a in roots; EjHSF-B4b in roots and inflorescence; EjHSF-C1b in stems and roots; EjHSF-A2a in three tissues; EjHSF-A8b in four tissues; and EjHSF-A4c, EjHSF-B1a, and EjHSF-B2b in six tissues. Moreover, fifteen EjHSFs were differentially expressed (eleven upregulated and four downregulated) in fruits under heat stress treatment in the color-changing period. Among them, EjHSF-A2a and EjHSF-A2b upregulated transcriptional abundance over 300 times after heat treatment; EjHSF-B2b consistently displayed an increasing trend over time; and EjHSF-B1a was significantly downregulated. Hence, these results suggest that EjHSFs take part in loquat tissue development and may be involved in the fruit’s heat stress response. These findings enhance the understanding of EjHSFs’ role within loquats and the heat stress response of its fruit and provide target genes for heat stress improvement.
Novel Sulfamethoxazole Organotin Complexes: Synthesis, Characterization, and Hydrogen Storage Application
Authors: Ahmed Dina S. , Emad Noor , Kadhom Mohammed , Yousif Emad , Al-Mashhadani Mohammed
Published: November 2024

This study presents the synthesis and characterization of novel sulfamethoxazole organotin complexes and evaluates their potential for hydrogen storage applications. The synthesized complexes were characterized using various techniques, such as Nuclear Magnetic Resonance and Fourier Transform Infrared spectroscopy to determine their constructional and physicochemical properties. Field Emission Scanning Electron Microscopy was applied to analyze the surface morphology, and the Brunauer–Emmett–Teller method was utilized to measure the surface area. High-pressure adsorption experiments demonstrated the remarkable hydrogen storage capabilities of these complexes, with the highest hydrogen uptake of 29.1 cm3/g observed at 323 K. The results suggest that the prepared sulfamethoxazole organotin complexes have the potential to be candidates for gas separation and storage applications.
Delineations for Police Patrolling on Street Network Segments with p-Median Location Models
Authors: Lee Changho , Kim Hyun , Chun Yongwan , Griffith Daniel A.
Published: November 2024

Police patrolling intends to enhance traffic safety by mitigating the risks associated with vehicle crashes and accidents. From a view of operations, patrolling requires an effective distribution of resources and often involves area delineations for this distribution purpose. Given constraints such as budget and human resources for traffic safety, delineating geographic areas optimally for police patrol areas is an important agenda item. This paper considers two p-median location models using segments on a street network as observational units on which traffic issues such as vehicle crashes occur. It also uses two weight sets to construct an enhanced delineation of police patrol areas in the City of Plano, Texas. The first model for the standard p-median formulation gives attention to the cumulative number of motor vehicle crashes from 2011 to 2021 on the major transportation networks in Plano. The second model, an extension of this first p-median one, uses balancing constraints to achieve balanced spatial coverage across patrol areas. These two models are also solved with network kernel density count estimates (NKDCE) instead of crash counts. These smoothed densities on a network enable consideration of uncertainty affiliated with this aggregation. The analysis results of this paper suggest that the p-median models provide effective specifications, including their capability to define patrol areas that encompass the entire study region while minimizing distance costs. The inclusion of balancing constraints ensures a more equitable distribution of workloads among patrol areas, improving overall efficiency. Additionally, the model with NKDCE results in an improved workload balance among delineated areas for police patrolling activities, thus supporting more informed spatial decision-making processes for public safety.
Monitoring and Treatment of Paroxysmal Nocturnal Hemoglobinuria in Patients with Aplastic Anemia in Asia: An Expert Consensus
Authors: Wong Raymond Siu Ming , Ho Jang Jun , Wong Lily Lee Lee , Kim Jin Seok , Rojnuckarin Ponlapat , Goh Yeow-Tee , Ueda Yasutaka , Chou Wen-Chien , Lee Jong Wook , Kanakura Yuzuru , Chiou Tzeon-Jye
Published: November 2024

Paroxysmal nocturnal hemoglobinuria (PNH) clones can be identified in a significant proportion of patients with aplastic anemia (AA). Screening for PNH clones at the time of an AA diagnosis is recommended by national and international guidelines. In this report, an expert panel of physicians discusses current best practices and provides recommendations for managing PNH in patients with AA in the Asia–Pacific region. Plasma/serum lactate dehydrogenase (LDH) levels and reticulocyte count should be measured with every blood test. PNH clone size should be monitored regularly by flow cytometry, with on-demand testing in the event of a rise in LDH level ± reticulocyte count or development of symptoms such as thrombosis. Monitoring for PNH clones can guide the choice of initial AA treatment, although flow cytometry has resource implications which may present a challenge in some Asia–Pacific countries. The treatment of patients with both PNH and AA depends on which condition predominates; following PNH treatment guidelines if hemolysis is the main symptom and AA treatment guidelines if bone marrow failure is severe (regardless of whether hemolysis is mild or moderate). The expert panel’s recommendations on the monitoring and treatment of PNH in patients with AA are practical for healthcare systems in the Asia–Pacific region.
FgGET3, an ATPase of the GET Pathway, Is Important for the Development and Virulence of Fusarium graminearum
Authors: Liu Caihong , Lei Lu , Zhu Jing , Chen Lirun , Peng Shijing , Zhang Mi , Zhang Ziyi , Tang Jie , Chen Qing , Kong Li , Zheng Youliang , Ladera-Carmona Maria , Kogel Karl-Heinz , Wei Yuming , Qi Pengfei
Published: November 2024

GET3 is an ATPase protein that plays a pivotal role in the guided entry of the tail-anchored (GET) pathway. The protein facilitates the targeting and inserting of tail-anchored (TA) proteins into the endoplasmic reticulum (ER) by interacting with a receptor protein complex on the ER. The role of GET3 in various biological processes has been established in yeast, plants, and mammals but not in filamentous fungi. Fusarium graminearum is the major causal agent of Fusarium head blight (FHB), posing a threat to the yield and quality of wheat. In this study, we found that FgGET3 exhibits a high degree of sequence and structural conservation with its homologs across a wide range of organisms. Ectopic expression of FgGET3 in yeast restored the growth defects of the Saccharomyces cerevisiae ScGET3 knock-out mutant. Furthermore, FgGET3 was found to dimerize and localize to the cytoplasm, similar to its homologs in other species. Deletion of FgGET3 in F. graminearum results in decreased fungal growth, fragmented vacuoles, altered abiotic stress responses, reduced conidia production, delayed conidial germination, weakened virulence on wheat spikes and reduced DON production. Collectively, these findings underscore the critical role of FgGET3 in regulating diverse cellular and biological functions essential for the growth and virulence of F. graminearum.
p53 and the E3 Ubiquitin Ligase MDM2 in Glaucomatous Lamina Cribrosa Cells
Authors: McElhinney Kealan , Irnaten Mustapha , O’Callaghan Jeffrey , O’Brien Colm
Published: November 2024

Lamina cribrosa (LC) cells play an integral role in extracellular matrix remodeling and fibrosis in human glaucoma. LC cells bear similarities to myofibroblasts that adopt an apoptotic-resistant, proliferative phenotype, a process linked to dysregulation of tumor suppressor-gene p53 pathways, including ubiquitin-proteasomal degradation via murine-double-minute-2 (MDM2). Here, we investigate p53 and MDM2 in glaucomatous LC cells. Primary human LC cells were isolated from glaucomatous donor eyes (GLC) and age-matched normal controls (NLC) (n = 3 donors/group). LC cells were cultured under standard conditions ± 48-h treatment with p53-MDM2-interaction inhibitor RG-7112. Markers of p53-MDM2, fibrosis, and apoptosis were analyzed by real-time polymerase chain reaction (qRT-PCR), western blotting, and immunofluorescence. Cellular proliferation and viability were assessed using colorimetric methyl-thiazolyl-tetrazolium salt assays (MTS/MTT). In GLC versus NLC cells, protein expression of p53 was significantly decreased (p < 0.05), MDM2 was significantly increased, and immunofluorescence showed reduced p53 and increased MDM2 expression in GLC nuclei. RG-7112 treatment significantly increased p53 and significantly decreased MDM2 gene and protein expression. GLC cells had significantly increased protein expression of αSMA, significantly decreased caspase-3 protein expression, and significantly increased proliferation after 96 h. RG-7112 treatment significantly decreased COL1A1 and αSMA, significantly increased BAX and caspase-3 gene expression, and significantly decreased proliferation in GLC cells. MTT-assay showed equivocal cellular viability in NLC/GLC cells with/without RG-7112 treatment. Our data suggests that proliferation and the ubiquitin-proteasomal pathway are dysregulated in GLC cells, with MDM2-led p53 protein degradation negatively impacting its protective role.
Acid Sphingomyelinase Activation and ROS Generation Potentiate Antiproliferative Effects of Mitomycin in HCC
Authors: Buitkamp Sirkka , Schwalm Stephanie , Jakobi Katja , Ferreiros Nerea , Wünsche Christin , Zeuzem Stefan , Gulbins Erich , Sarrazin Christoph , Pfeilschifter Josef , Grammatikos Georgios
Published: November 2024

Sphingolipids play a major role in the regulation of hepatocellular apoptosis and proliferation. We have previously identified sphingolipid metabolites as biomarkers of chronic liver disease and hepatocellular carcinoma. Human hepatocellular carcinoma cell lines were transfected with a plasmid vector encoding for acid sphingomyelinase. Overexpressing cells were subsequently treated with mitomycin and cell proliferation, acid sphingomyelinase activity, sphingolipid concentrations, and generation of reactive oxygen species were assessed. The stimulation of acid sphingomyelinase-overexpressing cell lines with mitomycin showed a significant activation of the enzyme (p < 0.001) followed by an accumulation of various ceramide species (p < 0.001) and reactive oxygen radicals (p < 0.001) as compared to control transfected cells. Consequently, a significant reduction in cell proliferation was observed in acid sphingomyelinase-overexpressing cells (p < 0.05) which could be diminished by the simultaneous application of antioxidant agents. Moreover, the application of mitomycin induced significant alterations in mRNA expression levels of ceramidases and sphingosine kinases (p < 0.05). Our data suggest that the overexpression of the acid sphingomyelinase in human hepatoma cell lines enhances the in vitro antiproliferative potential of mitomycin via accumulation of ceramide and reactive oxygen species. The selective activation of acid sphingomyelinase might offer a novel therapeutic approach in the treatment of hepatocellular carcinoma.
CAGn Polymorphic Locus of Androgen Receptor (AR) Gene in Russian Infertile and Fertile Men
Authors: Chernykh Vyacheslav , Solovova Olga , Sorokina Tatyana , Shtaut Maria , Sedova Anna , Bliznetz Elena , Ismagilova Olga , Beskorovainaya Tatiana , Shchagina Olga , Polyakov Aleksandr
Published: November 2024

The androgen receptor (AR) is critical for mediating the effects of androgens. The polymorphic CAGn locus in exon 1 of the AR gene is associated with several diseases, including spinal and bulbar muscular atrophy (SBMA), prostate cancer, and male infertility. This study evaluated the CAGn locus in 9000 infertile Russian men and 286 fertile men (control group). The CAGn locus was analyzed using the amplified fragment length polymorphism method. In the infertile cohort, the number of CAG repeats ranged from 6 to 46, with a unimodal distribution. The number of CAG repeats in infertile and fertile men was 22.15 ± 0.93 and 22.02 ± 1.36, respectively. In infertile men, variants with 16 to 29 repeats were present in 97% of the alleles. A complete mutation (≥42 CAG repeats) was found in three patients, while three others had 39-41 repeats. The incidence of SBMA was 1:3000 infertile men. Significant differences (p < 0.05) were observed between infertile and fertile men in alleles with 21, 24 and 25 repeats. This study revealed certain differences in the CAGn polymorphic locus of the AR gene in Russian infertile and fertile men and determined the frequency of SBMA in infertile patients.
Investigating p.Ala1035Val in NPC1: New Cellular Models for Niemann–Pick Type C Disease
Authors: David Hugo , Monfregola Jlenia , Ribeiro Isaura , Cardoso Maria Teresa , Sandiares Ana Catarina , Moreira Luciana , Coutinho Maria Francisca , Quelhas Dulce , Ballabio Andrea , Alves Sandra , Encarnação Marisa
Published: November 2024

Niemann–Pick type C (NPC) is a lysosomal storage disorder (LSD) caused by pathogenic variants in either the NPC1 or NPC2 genes, which encode proteins involved in the lysosomal export of unesterified cholesterol. In patients of Western European descent, the p.Ile1061Thr variant in NPC1 is especially prevalent. However, mounting evidence has positioned p.Ala1035Val as the most common variant in Portugal and the second most prevalent variant worldwide. By analyzing 10 Portuguese NPC patients homozygous for p.Ala1035Val, we found an SNP in cis on position 858 (p.Ile858Val), which we hypothesize could have a disease-modifying effect. To address this query, we created variant-specific in vitro models of NPC by stably transducing NPC1−/− ARPE-19 cells with constructs encoding different fluorescently-tagged variants of NPC1, which we used, alongside patient-derived skin fibroblasts, to investigate lysosomal positioning and the trafficking routes elicited by p.Ile1061Thr and p.Ala1035Val (with and without the p.Ile858Val SNP in cis). Our results corroborate the previously described decrease in p.Ile1061Thr-NPC1 trafficking to the lysosome and suggest a similar, if not worse, scenario for the p.Ala1035Val variant, especially when in cis with p.Ile858Val. This is the first reported functional study addressing the impact of the p.Ala1035Val variant at the cellular level, paving the way for novel therapeutic options.
Integrating Physiology, Transcriptome, and Metabolome Analyses Reveals the Drought Response in Two Quinoa Cultivars with Contrasting Drought Tolerance
Authors: Wang Yang , Wu Yang , Bao Qinghan , Shi Huimin , Zhang Yongping
Published: November 2024

Quinoa (Chenopodium quinoa Willd.) is an annual broadleaf plant belonging to the Amaranthaceae family. It is a nutritious food crop and is considered to be drought-tolerant, but drought is still one of the most important abiotic stress factors limiting its yield. Quinoa responses to drought are related to drought intensity and genotype. This study used two different drought-responsive quinoa cultivars, LL1 (drought-tolerant) and ZK1 (drought-sensitive), to reveal the important mechanisms of drought response in quinoa by combining physiological, transcriptomic, and metabolomic analyses. The physiological analysis indicated that Chla/Chlb might be important for drought tolerance in quinoa. A total of 1756 and 764 differentially expressed genes (DEGs) were identified in LL1 and ZK1, respectively. GO (Gene Ontology) enrichment analysis identified 52 common GO terms, but response to abscisic acid (GO:0009737) and response to osmotic stress (GO:0006970) were only enriched in LL1. KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis revealed that glycerophospholipid metabolism (ko00564) and cysteine and methionine metabolism (ko00270) ranked at the top of the list in both cultivars. A total of 1844 metabolites were identified by metabolomic analysis. “Lipids and lipid-like” molecules had the highest proportions. The DEMs in LL1 and ZK1 were mainly categorized 6 and 4 Human Metabolome Database (HMDB) superclasses, respectively. KEGG analysis revealed that the ‘α-linolenic acid metabolism’ was enriched in both LL1 and ZK1. Joint KEGG analysis also revealed that the ‘α-linolenic acid metabolism’ pathway was enriched by both the DEGs and DEMs of LL1. There were 17 DEGs and 8 DEMs enriched in this pathway, and methyl jasmonate (MeJA) may play an important role in the drought response of quinoa. This study will provide information for the identification of drought resistance in quinoa, research on the molecular mechanism of drought resistance, and genetic breeding for drought resistance in quinoa.
Association Between Zinc Status and Insulin Resistance/Sensitivity Check Indexes in Gestational Diabetes Mellitus
Authors: Genova Mariana P. , Ivanova Irena , Naseva Emilia , Atanasova Bisera
Published: November 2024

Gestational diabetes mellitus (GDM) is considered the most common metabolic disorder of the pregnancy period. It is characterized by pancreatic beta-cell dysfunction in the setting of chronic insulin resistance. Zinc is a nutrient involved in numerous metabolic processes and shows a relationship with glycometabolic disorders and GDM. The latest data have demonstrated the association of zinc with insulin sensitivity and resistance. The exact role of zinc in the connection with indexes of insulin resistance and insulin sensitivity is still not fully clarified. The aim of the study is to analyze the newly calculated indexes Glu/Zn, Ins/Zn, and HOMA-IR/Zn as surrogate markers to explore the correlation between serum zinc status and some indexes of insulin sensitivity and insulin resistance. The possible role of these indexes as markers of insulin resistance in pregnant women was analyzed too. An ROC analysis demonstrated that HOMA-IR/Zn with AUC 0.989, p < 0.001 (95% CI 0.967–1.000) and Ins/Zn with AUC 0.947, p < 0.001 (95% CI 0.889–1.000) in the GDM group, and only HOMA-IR/Zn index with AUC 0.953, p < 0.001 (95% CI 0.877–1.000) in healthy pregnant women, have good power as markers of insulin resistance in both groups. We speculate that these new ratios could be suitable for the assessment of pregnant women at high risk of insulin resistance development and, probably, for the evaluation of the specific pathophysiologic characteristics of women with GDM.
Oxidative Stress and Placental Pathogenesis: A Contemporary Overview of Potential Biomarkers and Emerging Therapeutics
Authors: Vornic Ioana , Buciu Victor , Furau Cristian George , Gaje Pusa Nela , Ceausu Raluca Amalia , Dumitru Cristina-Stefania , Barb Alina Cristina , Novacescu Dorin , Cumpanas Alin Adrian , Latcu Silviu Constantin , Cut Talida Georgiana , Zara Flavia
Published: November 2024

Oxidative stress (OS) plays a crucial role in placental pathogenesis and pregnancy-related complications. This review explores OS’s impact on placental development and function, focusing on novel biomarkers for the early detection of at-risk pregnancies and emerging therapeutic strategies. We analyzed recent research on OS in placental pathophysiology, examining its sources, mechanisms, and effects. While trophoblast invasion under low-oxygen conditions and hypoxia-induced OS regulate physiological placental development, excessive OS can lead to complications like miscarriage, preeclampsia, and intrauterine growth restriction. Promising OS biomarkers, including malondialdehyde, 8-isoprostane, and the sFlt-1/PlGF ratio, show potential for the early detection of pregnancy complications. Therapeutic strategies targeting OS, such as mitochondria-targeted antioxidants, Nrf2 activators, and gasotransmitter therapies, demonstrate encouraging preclinical results. However, clinical translation remains challenging. Future research should focus on validating these biomarkers in large-scale studies and developing personalized therapies to modulate placental OS. Emerging approaches like extracellular vesicle-based therapies and nanomedicine warrant further investigation for both diagnostic and therapeutic applications in pregnancy-related complications. Integrating OS biomarkers with other molecular and cellular markers offers improved potential for the early identification of at-risk pregnancies.
Systematic Review on Additive Friction Stir Deposition: Materials, Processes, Monitoring and Modelling
Authors: Yasa Evren , Poyraz Ozgur , Molyneux Anthony , Sharman Adrian , Bilgin Guney Mert , Hughes James
Published: November 2024

Emerging solid-state additive manufacturing (AM) technologies have recently garnered significant interest because they can prevent the defects that other metal AM processes may have due to sintering or melting. Additive friction stir deposition (AFSD), also known as MELD, is a solid-state AM technology that utilises bar feedstocks as the input material and frictional–deformational heat as the energy source. AFSD offers high deposition rates and is a promising technique for achieving defect-free material properties like wrought aluminium, magnesium, steel, and titanium alloys. While it offers benefits in terms of productivity and material properties, its low technology readiness level prevents widespread adoption. Academics and engineers are conducting research across various subfields to better understand the process parameters, material properties, process monitoring, and modelling of the AFSD technology. Yet, it is also crucial to compile and compare the research findings from past studies on this new technology to gain a comprehensive understanding and pinpoint future research paths. This paper aims to present a comprehensive review of AFSD focusing on process parameters, material properties, monitoring, and modelling. In addition to examining data from existing studies, this paper identifies areas where research is lacking and suggests paths for future research efforts.
Reduced Salivary Gustin and Statherin in Long-COVID Cohort with Impaired Bitter Taste
Authors: Chowdary Harika , Riley Naomi , Patel Parul , Gossweiler Ana G. , Running Cordelia A. , Srinivasan Mythily
Published: November 2024

Background/Objectives: Taste dysfunction is a frequent symptom of acute coronavirus disease (COVID)-19 caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). While the majority of those affected reported recovery over time, emerging data suggest that 20–25% of individuals experience persistent taste dysfunction, constituting a common symptom of long COVID. Gustation is mediated by continuously renewing taste bud cells. A balance between the counteracting processes of cell generation and cell death maintains the homeostatic turnover. Sonic hedgehog (SHH) is a morphogenic protein that promotes taste cell proliferation and differentiation. Enzymatic proteins such as gustin modulate the environment around the taste receptors and influence taste perception. Hence, we hypothesized that increased taste cell turnover and reduced taste-related salivary proteins contribute to the taste dysfunction in long COVID. Methods: Unstimulated whole saliva (UWS) was collected from individuals with long COVID experiencing taste dysfunction after obtaining informed consent. The normal control included archived saliva samples catalogued prior to 2019. Taste perception was objectively determined by the waterless empirical taste test. The SHH, gustin, and inflammatory cytokines in UWS were determined with ELISA. The expressions of epithelial and taste-cell-specific markers in cellular saliva were assessed by immunoflurorescence. Results: Impaired bitter taste was the most common dysfunction in the long-COVID cohort. Salivary gustin was significantly lower in those with long COVID and correlated with lower bitter taste score. Cellular saliva showed keratin-10- and small-proline-rich protein-positive epithelial cells as well as SHH-, occluding- and KCNQ1-positive taste cells. Conclusions: Salivary gustin could be a marker for impaired bitter taste in long COVID.
The Influence of the Amount of Technological Waste on the Performance Properties of Fibrous Polymer Composites
Authors: Dobránsky Jozef , Gombár Miroslav , Fejko Patrik
Published: November 2024

The objective of the experimental analysis was to assess the impact of the reuse of technological waste (recyclate) on the selected performance properties of the fibrous polymer composite used to produce components for the automotive industry by injection molding technology. Polyphthalamide (PPA), which belongs to a group of high-tech polymers, was chosen as the analyzed material. In accordance with the set goals, the rheological, mechanical, and structural properties of the material were evaluated using ANOVA analysis in the experimental part of the work, depending on the mass ratio of the recycled material added to the virgin material. The influence of the proportion of recycled material on the lifetime of moldings by the method of their exposure at an elevated temperature for a defined time was also assessed. During the research, it was found that at a concentration of up to 40 wt. % of recyclate, its mechanical properties do not change significantly. At a concentration of 50 wt. %, there is a rapid decrease in mechanical properties. In the long term, it can also be said that the addition of recyclate significantly affects the service life of the components. No significant changes in morphology were observed during the analysis of structural properties.
Laser-Induced Decomposition and Mechanical Degradation of Carbon Fiber-Reinforced Polymer Subjected to a High-Energy Laser with Continuous Wave Power up to 120 kW
Authors: Schäffer Sebastian , Reich Stefan , Heunoske Dominic , Lueck Martin , Wolfrum Johannes , Osterholz Jens
Published: November 2024

Carbon fiber-reinforced polymer (CFRP), noted for its outstanding properties including high specific strength and superior fatigue resistance, is increasingly employed in aerospace and other demanding applications. This study investigates the interactions between CFRP composites and high-energy lasers (HEL), with continuous wave laser powers reaching up to 120 kW. A novel automated sample exchange system, operated by a robotic arm, minimizes human exposure while enabling a sequence of targeted laser tests. High-speed imaging captures the rapid expansion of a plume consisting of hot gases and dust particles during the experiment. The research significantly advances empirical models by systematically examining the relationship between laser power, perforation times, and ablation rates. It demonstrates scalable predictions for the effects of high-energy laser radiation. A detailed examination of the damaged samples, both visually and via micro-focused computed X-ray tomography, offers insights into heat distribution and ablation dynamics, highlighting the anisotropic thermal properties of CFRP. Compression after impact (CAI) tests further assess the residual strength of the irradiated samples, enhancing the understanding of CFRP’s structural integrity post-irradiation. Collectively, these tests improve the knowledge of the thermal and mechanical behavior of CFRP under extreme irradiation conditions. The findings not only contribute to predictive modeling of CFRP’s response to laser irradiation but enhance the scalability of these models to higher laser powers, providing robust tools for predicting material behavior in high-performance settings.
Finite Element Modelling of Circular Concrete-Filled Steel Tubular Columns Under Quasi-Static Axial Compression Loading
Authors: Almasabha Ghassan , Ramadan Mohammad
Published: November 2024

This paper presents a modified finite element analysis (FEA) model for predicting the axial compression strength of large-diameter concrete-filled steel tubular (CFST) stub columns, addressing the gap in research that has often focused on smaller diameters. The size effect, which significantly impacts the structural performance of large-diameter CFST columns, is a key focus of this study. The goal is to validate the accuracy and reliability of the modified FEA model by comparing its predictions with experimental data from the literature, specifically examining ultimate axial load capacity, failure modes, and deformed shapes. In addition to validating the model, this study includes a comprehensive parametric analysis that explores how critical geometric parameters such as the diameter-to-thickness (D/t) ratio and length-to-diameter (L/D) ratio affect the axial compressive behavior of CFST stub columns. By systematically varying these parameters, the research provides valuable insights into the load-bearing capacity, deformation characteristics, and failure mechanisms of CFST columns. Furthermore, the material properties of the steel tube—particularly its yield strength—and the compressive strength of the concrete core are investigated to optimize the design and safety performance of these columns. The results indicate that the FEA model shows excellent agreement with experimental results, accurately predicting the axial load-strain response. It was observed that as the diameter of the steel tube increases, the peak stress, peak strain, strength index, and ductility index tend to decrease, underscoring the size effect. Conversely, an increase in the yield strength and thickness of the steel tube enhances the ultimate strength of the CFST columns. These findings demonstrate the reliability of the modified FEA model in predicting the behavior of large-diameter CFST columns, offering a useful tool for optimizing designs and improving safety margins in structural applications.
Changes in Exercise Performance in Patients During a 6-Week Inpatient Psychiatric Rehabilitation Program and Associated Effects on Depressive Symptoms
Authors: Birnbaumer Philipp , Traunmüller Claudia , Natmessnig Christian , Senft Birgit , Jaritz Caroline , Hochfellner Sigurd , Schwerdtfeger Andreas , Hofmann Peter
Published: November 2024

Background/Objectives: The impact of exercise on affective disorders has been demonstrated in various studies. However, almost no data are available on performance effects. Therefore, this study investigated exercise performance related to the severity of depression symptoms in a pre–post within-subjects design in a 6-week standard inpatient psychiatric rehabilitation program. Methods: A total of 53 individuals (20 female; mean age, 40.98 ± 11.33) with a primary diagnosis of depression performed a cardiopulmonary exercise test (CPX) to obtain maximal oxygen uptake (VO2max), maximal power output (Pmax), and the first and second ventilatory threshold (VT1, VT2) at the start and the end of the rehabilitation. Degree of depression was assessed by Becks Depression Inventory (BDI) and the Brief Symptom Inventory test (BSI). Overall activity was measured by accelerometer step-counts. Results: Mean total step-count per day during rehabilitation was high (12,586 ± 2819 steps/day). Patients’ BDI and BSI at entry were 21.6 ± 8.83 and 65.1 ± 6.8, respectively, and decreased significantly (p < 0.001) following rehabilitation, to 10.1 ± 9.5 and 54.5 ± 11.3, respectively. Pmax and VO2max increased significantly (p < 0.001) from entry values (182.6 ± 58.7 W, 29.74 ± 5.92 mL·kg−1·min−1) following rehabilitation: by 11.91 ± 12.09 W and 1.35 ± 2.78 mL·kg−1·min−1, respectively. VT1 and VT2 showed a similar behavior. An increase in physical performance could predict improvement in BDI (R2 = 0.104, F(1,48) = 5.582, p = 0.022) but not in BSI. Conclusions: The program was associated with improved mental health status in all patients and increased performance in the majority of patients, although increases were small. Since improvements in exercise performance may be positively related to depression symptoms and comorbidities, it is recommended to individualize and tailor exercise programs, which could yield larger effects.
Minimum Normalized Cycling Cadence to Increase Post-Cycling Gait Velocity
Authors: Lama Nitu , Keating Christopher J. , Donahue Paul T. , Oliveira Nuno , Thorsen Tanner A.
Published: November 2024

Background: Previous research has shown that increasing cycling cadence can result in improved post-cycling gait velocity. However, the specific threshold of cycling cadence required to bring about clinically meaningful changes in gait velocity remains unknown. This study aimed to determine the minimum increment in cycling cadence that would lead to a significant improvement in post-cycling gait velocity. Methods: A total of 42 young adults participated in our study and were randomly assigned to one of three groups: TEN, TWENTY, and THIRTY. Each group was assigned to cycle at a cadence at the corresponding percentage higher than the participant’s self-selected gait cadence. Each participant engaged in a 15-min cycling session at their respective assigned cycling cadence. Before and after cycling, participants completed a 10-meter walk test while spatiotemporal parameters of gait, ground reaction forces, lower extremity kinematics, and kinetics were recorded. Results: One-way ANOVA revealed no statistically significant changes in spatiotemporal, ground reaction force, kinematics, and kinetics variables pre- and post-cycling. However, there were both statistically significant (F(2,41) = 3.794, p = 0.031, η2 = 0.604) and clinically meaningful changes (0.07 m/s) in post-cycling gait velocity in the THIRTY group only. Conclusions: This suggests that a cycling cadence of 30% or higher is the minimum requirement to produce a clinically significant improvement in gait velocity.
Major Choices: Students’ Personal Intelligence, Considerations When Choosing a Major, and Academic Success
Authors: Sylaska Kateryna , Mayer John D.
Published: November 2024

A student’s choice of major is influenced by their parents and peers, as well as by the quality of the college department that offers the major and by broader cultural and economic issues. The student’s own personality, including their ability to reason about themselves and their interests, also contributes to the choice and its outcomes. In a preliminary study, we developed a Choice of Major Scale that depicts key aspects of students’ consideration of their major. Then, across three studies (Ns = 304, 466, and 492), we examined the students’ personal intelligence, defined as their capacity to reason about their own and other people’s personalities, as well as a Choice of Major Scale, and the relation of those variables to important academic outcomes. The results depicted a pattern that the students who better understood personality and were more engaged in choosing a major, compared to others, considered more majors initially, chose a major more quickly, and exhibited better academic outcomes subsequently.
An Experimental Investigation into the Enhancement of Surface Quality of Inconel 718 Through Axial Ultrasonic Vibration-Assisted Grinding in Dry and MQL Environments
Authors: Das Sreethul , Chinnaiyan Pandivelan , Jayaseelan Joel , Paulchamy Jeyapandiarajan , Batako Andre , Pazhani Ashwath
Published: November 2024

Ultrasonic vibration-assisted grinding (UVAG) has proven to be beneficial for grinding difficult-to-machine materials. This work attempts to enhance the grinding performance of Inconel 718 through a comprehensive study of UVAG characteristics. Grinding experiments were performed in both dry and Minimum Quantity Lubrication (MQL) environments, and assessment of the grinding forces, specific energy, residual stress, and surface topography was done. A substantial reduction of both surface roughness and grinding force components was observed in UVAG compared to conventional grinding (CG). Utilizing UVAG with MQL at the maximum vibration amplitude led to a 64% reduction in tangential grinding force and a 51% decrease in roughness parameter, Ra, when compared to CG conducted in a dry environment. The high-frequency indentations of the abrasives in UVAG generated compressive residual stresses on the ground surface. Surface parameters pointed to uniform texture and SEM images showed widening of abrasive grain tracks on the workpiece surface during UVAG. The utilization of UVAG under MQL produced a synergistic impact and resulted in the lowest grinding forces, specific energy, and optimal surface quality among all the grinding conditions investigated. Overall analysis of the results indicated that the axial configuration of the vibration set-up is favorable for UVAG, and the high-frequency periodic separation-cutting characteristic of the process improves lubricating efficiency and grinding performance.
Geometric and Mechanical Properties of Ti6Al4V Skeletal Gyroid Structures Produced by Laser Powder Bed Fusion for Biomedical Implants
Authors: Hou Cong , Goris Max , Rosseel Dries , Vrancken Bey , Denis Kathleen
Published: November 2024

Skeletal gyroid structures possess promising applications in biomedical implants, owing to their smooth and continuously curved surfaces, open porosity, and customisable mechanical properties. This study simulated the geometric properties of Ti6Al4V skeletal gyroid structures, with relative densities ranging from 1.83% to 98.17%. The deformation behaviour of these structures was investigated through a combination of uniaxial compression tests and simulations, within a relative density range of 13.33% to 50% (simulation) and 15.19% to 41.69% (experimental tests). The results established explicit analytical correlations of pore size and strut diameter with the definition parameters of the structures, enabling precise control of these dimensions. Moreover, normalised Young’s modulus (ranging from 1.05% to 20.77% in simulations and 1.65% to 15.53% in tests) and normalised yield stress (ranging from 1.75% to 17.39% in simulations and 2.09% to 13.95% in tests) were found to be power correlated with relative density. These correlations facilitate the design of gyroid structures with low stiffness to mitigate the stress-shielding effect. The presence of macroscopic 45° fractures in the gyroid structures confirmed that the primary failure mechanism is induced by shear loads. The observed progressive failure and plateau region proved the bending-dominant behaviour and highlighted their excellent deformability. Additionally, the anisotropy of gyroid structures was confirmed through variations in stress and strain concentrations, deformation behaviour, and Young’s modulus under different loading directions.
Numerical Modeling on Ocean-Bottom Seismograph P-Wave Receiver Function to Analyze Influences of Seawater and Sedimentary Layers
Authors: Gong Wenfei , Hu Hao , Ruan Aiguo , Niu Xiongwei , Wang Wei , Tang Yong
Published: November 2024

It is challenging to apply the receiver function method to teleseisms recorded by ocean-bottom seismographs (OBSs) due to a specific working environment that differs from land stations. Teleseismic incident waveforms reaching the area beneath stations are affected by multiple reflections generated by seawater and sediments and noise resulting from currents. Furthermore, inadequate coupling between OBSs and the seabed basement and the poor fidelity of OBSs reduce the signal-to-noise ratio (SNR) of seismograms, leading to the poor quality of extracted receiver functions or even the wrong deconvolution results. For instance, the poor results cause strong ambiguities regarding the Moho depth. This study uses numerical modeling to analyze the influences of multiple reflections generated by seawater and sediments on H-kappa stacking and the neighborhood algorithm. Numerical modeling shows that seawater multiple reflections are mixed with the coda waves of the direct P-wave and slightly impact the extracted receiver functions and can thus be ignored in subsequent inversion processing. However, synthetic seismograms have strong responses to the sediments. Compared to the waveforms of horizontal and vertical components, the sedimentary responses are too strong to identify the converted waves clearly. The extracted receiver functions correspond to the above influences, resulting in divergent results of H-kappa stacking (i.e., the Moho depth and crustal average VP/VS ratio are unstable and have great uncertainties). Fortunately, waveform inversion approaches (e.g., the neighborhood algorithm) are available and valid for obtaining the S-wave velocity structure of the crust–upper mantle beneath the station, with sediments varying in thickness and velocity.
An Integrated Approach to Riverbed Morphodynamic Modeling Using Remote Sensing Data
Authors: Bozzano Matteo , Varni Francesco , De Martino Monica , Quarati Alfonso , Tambroni Nicoletta , Federici Bianca
Published: November 2024

River inlets, deltas, and estuaries represent delicate ecosystems highly susceptible to climate change impacts. While significant progress has been made in understanding the morphodynamics of these environments in recent decades, the development of models still requires thorough testing and data integration. In this context, remote sensing emerges as a potent tool, providing crucial data and the ability to monitor temporal changes. In this paper, an integrated approach combining remote sensing and morphodynamic modeling is proposed to assess river systems comprehensively. By utilizing multispectral or RGB optical imagery from satellites or UAVs for river classification and remotely derived bathymetry, echo sounder data for ground truth, and photogrammetric modeling of emerged areas, we outline a procedure to create an integrated and continuous digital terrain model (DTM) of a riverbed, paying particular attention to the wet–dry interface. This method enables us to identify the river centerline, its width, and its slope variations. Additionally, by applying a linear morphodynamic model that considers the spatial variability of river morphology commonly found in estuarine environments, it is possible to predict the wavelength and migration rate of sediment bars. This approach has been successfully applied to recreate the DTM and monitor the morphodynamics of the seaward reach of the Roya River (Italy).
Short-Term Flow Velocity Stress on the Behavioral, Physiological, and Biochemical Responses of the Large Yellow Croaker (Larimichthys crocea)
Authors: Qian Zhenjia , Xu Jincheng , Liu Huang , Zhang Chenglin , Cui Mingchao
Published: November 2024

Flow velocity is a key environmental factor that affects the behavioral strategies and physiological homeostasis of fish. To study the effects of flow velocity on the behavioral changes and blood physiology of the yellow croaker, the behavioral patterns of yellow croakers in response to flow velocity stress were obtained by analyzing changes in tail wagging frequency and amplitude. Differences in blood glucose, lactate, and cortisol were compared to determine their appropriate flow rate ranges. The juvenile stage of the large yellow croaker is crucial, as environmental changes can affect the physiology of fish. Therefore, juvenile yellow croakers were selected as the experimental subjects for this study. Twenty-four healthy and uniformly sized large yellow croakers with body weights of (90.26 ± 9.91) g and body lengths of (19.91 ± 0.69) cm were randomly assigned to one control group and three experimental groups, with five fish in each group. The experimental group was set with three flow rates, namely 1 bl/s (20 cm/s), 2 bl/s (40 cm/s), and 3 bl/s (60 cm/s), with a flow rate stress duration of 1 h. The results showed that: (1) Under different flow velocities, the fish exhibited different tail wagging patterns. At low flow velocities, their tail fins exhibited a “C”-shaped swing, while at high flow velocities, their bodies exhibited an “S”-shaped swing. (2) Oscillation frequency and amplitude both increased with increasing flow velocity. At a flow velocity of 2 bl/s, the oscillation frequency significantly increased. When the flow velocity reached 3 bl/s, the oscillation amplitude significantly increased (p < 0.05). (3) Blood physiology showed significant changes with increased flow rate, and blood glucose content continuously decreased with increased flow rate, significantly decreasing at a flow rate of 2 bl/s (p < 0.05). Lactic acid and cortisol both increased with increasing flow rate, and significantly increased at a flow rate of 3 bl/s (p < 0.05). In summary, under high-flow velocity stress, significant changes occurred in the behavior and physiology of large yellow croakers, which were consistent with physiological changes in the blood. A flow rate higher than 2 bl/s can lead to intense swimming behavior, decreased blood sugar concentration, and increased lactate accumulation and stress levels. Therefore, the short-term tolerance of yellow croakers is 2 bl/s, and a flow rate of 1 bl/s is more suitable.
Time Domain Design of a Marine Target Tracking System Accounting for Environmental Disturbances
Authors: Fruzzetti Camilla , Buzzurro Cristiana , Donnarumma Silvia , Martelli Michele
Published: November 2024

Environmental disturbances represent significant challenges to the performance and accuracy of autonomous systems, especially in marine environments, where their impact varies based on disturbance severity and the employed guidance law. This paper comprehensively investigates a marine target tracking system using time-domain simulations incorporating realistic environmental disturbances. Three guidance laws and four key performance indicators are analysed to evaluate system performance under disturbed and ideal conditions. A robust and systematic evaluation pipeline is developed and applied to a case study featuring a scaled tugboat model. This approach provides a reliable method to assess tracking accuracy and robustness in adverse conditions. The results are selected from a wide range of possibilities to show the effect of the disturbances on the selected target tracking motion control scenario with two manoeuvres and two environmental conditions. The results are measured through the selected key performance indicators, and several phases are identified for each manoeuvre to extend the analysis not only to the global KPI values but also to the partial values of defined phases. They reveal the quantitative effects of environmental disturbances, exposing different system behaviours and trends. These findings demonstrate the effectiveness of the proposed pipeline in quantifying tracking system performance, delivering useful understandings of the system under environmental disturbances. The broader implications of this study are substantial, offering enhanced predictive accuracy for the performance of the analysed systems, particularly in the context of target tracking. Furthermore, introducing numerical key performance indicators facilitates a more rigorous comparison of different system characteristics, enabling informed decisions in designing and optimising autonomous operations in challenging environments.