bluuebunny/crossref_metadata_2025 huggingface dataset:
Cautery Disbudding Iron Application Time and Brain Injury in Goat Kids: A Pilot Study
Authors: Hempstead Melissa N. , Shearer Jan K. , Sutherland Mhairi A. , Fowler Jennifer L. , Smith Joseph S. , Smith Jodi D. , Lindquist Taylor M. , Plummer Paul J.
Published: January 2021

Cautery disbudding is a painful procedure performed on goat kids to prevent horn growth that may result in brain injury. Thermal damage to the cerebral cortex of the brain and subsequent neurologic disease is a primary concern. Cautery iron application time may affect transmission of heat to the brain; however, research in this area is scarce. Therefore, the objective of this pilot study was to evaluate the effect of iron application time on brain injury of goat kids. A total of six buck and doe kids <9 days of age were obtained from a commercial dairy and transported to an Iowa State University research facility. Kids received a different randomly assigned application time (5, 10, 15, or 20s) on each horn bud. Kids were disbudded using an electric cautery iron (under isoflurane general anesthesia). After a 5-day observation period, the kids were euthanized, and magnetic resonance (MR) images were acquired to evaluate brain injury. Additionally, four of the six kids were presented for gross examination and two kids were selected for histopathologic examination. From the MR images, white matter edema was observed subjacent to four treated areas, representing application times of 5 s (one horn bud), 15 s (one horn bud), and 20 s (two horn buds). With the exception of the horn bud that received 5 s, which had white matter edema restricted to a single gyrus, the remaining three groups had a branching region of edema. No bone abnormalities were identified on any kids. Gross evidence of discoloration and hemorrhage on the cerebral hemispheres was observed on two horn buds that received 20 s, two horn buds that received 15 s, and one horn bud that received 10 s. Microscopic lesions consisting of leptomeningeal and cerebrocortical necrosis were observed in sections of brain from all groups. Lesions were most severe with 20 s. In conclusion, all application times used in this study resulted in some level of brain injury; however, using 15 s or more resulted in more severe and consistent brain injury. These results indicate that extended iron application time may increase the risk of brain injury in cautery disbudded kids.
Effects of Thermal Conditioning on Changes in Hepatic and Muscular Tissue Associated With Reduced Heat Production and Body Temperature in Young Chickens
Authors: Ouchi Yoshimitsu , Chowdhury Vishwajit S. , Cockrem John F. , Bungo Takashi
Published: January 2021

Effects of increased summer temperatures on poultry production are becoming more pronounced due to global warming, so it is important to consider approaches that might reduce heat stress in chickens. Thermal conditioning in chickens in the neonatal period can improve thermotolerance and reduce body temperature increases when birds are exposed to high ambient temperature later in life. The objective of this study was to investigate physiological and molecular changes associated with heat production and hence body temperature regulation under high ambient temperatures in thermally conditioned chicks. Three-day-old broiler chicks (Chunky) were thermally conditioned by exposure to a high ambient temperature (40°C) for 12 h while control chicks were kept at 30°C. Four days after the treatment, both groups were exposed to 40°C for 15 or 90 min. The increase in rectal temperature during 90 min of exposure to a high ambient temperature was less in thermally conditioned than control chicks. At 15-min of re-exposure treatment, gene expression for uncoupling protein and carnitine palmitoyletransferase 1, key molecules in thermogenesis and fatty acid oxidation, were significantly higher in pectoral muscle of control chicks but not conditioned chicks. Hepatic argininosuccinate synthase (ASS) decreased and hepatic argininosuccinate lyase (ASL) increased after reexposure to a high temperature. The concentrations of hepatic arginosuccinic acid, and ASS and ASL expression, were upregulated in conditioned chicks compared with the control chicks, indicating activity of the urea cycle could be enhanced to trap more energy to reduce heat production in conditioned chicks. These results suggest thermal conditioning can reduce the increase in heat production in muscles of chickens that occurs in high ambient temperatures to promote sensible heat loss. Conditioning may also promote energy trapping process in the liver by altering the heat production system, resulting in an alleviation of the excessive rise of body temperature.
Reducing Antimicrobial Use by Implementing Evidence-Based, Management-Related Prevention Strategies in Dairy Cows in Switzerland
Authors: Gerber Manuela , Dürr Salome , Bodmer Michèle
Published: January 2021

The aim of this study was to reduce antimicrobial use (AMU) on dairy farms that implemented evidence-based management strategies. The study not only examined whether these strategies led to a reduction in AMU in practice, but also examined the influence of the level of their implementation on AMU. For data analysis, practice software recordings of the farm veterinarians were used. The AMU data of 50 farms with prevention strategies applied (intervention group, IG) over 3 years (2017–2019) and of 74 farms without prevention strategies (control group, CG) over 2 years (2018–2019) were analyzed. Project participation was supported with 500 Swiss francs (~545 USD) per farmer per year. The AMU was compared between the IG and CG using the treatment incidence. In December 2017/January 2018, the farmers of the IG had chosen at least one of the proposed 17 prevention strategies from one of three sectors, i.e., udder health, uterine health and/or replacement calf health. The prevention strategies, were developed in a standard operating procedure protocol and were discussed in detail with the farmers before the implementation. Forty-eight farms chose at least one udder strategy, 10 farms at least one uterine strategy and 37 farms at least one calf strategy. By choosing an udder health strategy or a uterine health strategy, the corresponding systemically administered AMU could be significantly reduced (p < 0.04) in the IG compared with the CG. In addition, udder strategies that were well-implemented led to a significant reduction (p = 0.05) of intramammary “highest priority critically important antimicrobials (HPCIA)” (quinolones, cephalosporins 3rd and higher generation, macrolides and ketolides, glycopeptides, and polymyxins). The level of implementation was significantly lower in 2019 compared to 2018 (p < 0.05, Fisher's exact test). No significant reduction in AMU could be achieved for the calf sector. A reduction of AMU in dairy farms is possible by implementing evidence-based management-related prevention strategies. The level of implementation has only an influence on the consumption of HPCIA. The reduction of AMU in practice by means of evidence-based measures requires supportive human resources instead of financial support, because financial support for farmers seems not to motivate them sufficiently.
Dynamic Characterization of a High-Altitude Balloon during a Flight Campaign for the Detection of ISM Radio Background in the Stratosphere
Authors: Gemignani Matteo , Marcuccio Salvo
Published: January 2021

Sounding balloons, available at very low cost from commercial vendors and operable with minimal training, have an excellent potential as testing platforms in the near-space environment. The work reported here was motivated by the need to perform an experimental assessment of the radio frequency (RF) background present in the ISM (Industrial, Scientific and Medical) bands, namely 868 MHz (Ultra High Frequency—UHF) and 2.4 GHz (S-Band), simulating the operational environment of a Low Earth Orbit (LEO) constellation forInternet of Things (IoT) telecommunications. To this end, five balloons were flown with a dedicated RF payload. Along with the radio measurements, the flights provided a convenient opportunity to collect data on the dynamic behavior of the payload gondola. We report on the system design and the operational phase of the mission, and discuss the data collected throughout the successful flight campaign. As a result, a preliminary understanding of the gondola dynamics has been gained, including both linear accelerations and attitude oscillations. It is also concluded that the two ISM bands considered are actually suitable for IoT ground-to-LEO links.
Genome-Wide Association Study Using a Multiparent Advanced Generation Intercross (MAGIC) Population Identified QTLs and Candidate Genes to Predict Shoot and Grain Zinc Contents in Rice
Authors: Liu Shilei , Zou Wenli , Lu Xiang , Bian Jianmin , He Haohua , Chen Jingguang , Ye Guoyou
Published: January 2021

Zinc (Zn) is an essential trace element for the growth and development of both humans and plants. Increasing the accumulation of Zn in rice grains is important for the world’s nutrition and health. In this study, we used a multiparent advanced generation intercross (MAGIC) population constructed using four parental lines and genotyped using a 55 K rice SNP array to identify QTLs related to Zn2+ concentrations in shoots at the seedling stage and grains at the mature stage. Five QTLs were detected as being associated with shoot Zn2+ concentration at the seedling stage, which explained 3.7–5.7% of the phenotypic variation. Six QTLs were detected as associated with grain Zn2+ concentration at the mature stage, which explained 5.5–8.9% of the phenotypic variation. Among the QTLs, qSZn2-1/qGZn2 and qSZn3/qGZn3 were identified as being associated with both the shoot and grain contents. Based on gene annotation and literature information, 16 candidate genes were chosen in the regions of qSZn1, qSZn2-1/qGZn2, qSZn3/qGZn3, qGZn7, and qGZn8. Analysis of candidate genes through qRT-PCR, complementation assay using the yeast Zn-uptake-deficient double-mutant ZHY3, and sequencing of the four parental lines suggested that LOC_Os02g06010 may play an important role in Zn2+ accumulation in indica rice.
Phytochemical and Functional Characterization of Phenolic Compounds from Cowpea (Vigna unguiculata (L.) Walp.) Obtained by Green Extraction Technologies
Authors: Avanza M. Victoria , Álvarez-Rivera Gerardo , Cifuentes Alejandro , Mendiola José A. , Ibáñez Elena
Published: January 2021

This work presents a green, downstream process, from extraction to phytochemical characterization and bioactivity testing, to obtain and evaluate the functional properties of phenolic compounds from cowpea (Vigna unguiculata (L.) Walp.) seeds and pods. Phenolic-rich extracts were obtained by pressurized liquid extraction (PLE). The main factors affecting the extraction conditions (temperature and solvent) were optimized in order to attain extracts with the highest extraction yield, antioxidant capacity, and total phenolic content. The optimal extraction conditions were 1:1 ethanol:water at 170 °C with one extraction cycle for seeds and three extraction cycles for pods. Phenolic compounds of optimal extract were analyzed by UHPLC-q-TOF-MS/MS (quadrupole-time of flight tandem MS). The obtained PLE-extracts exhibited higher phenolic content and antioxidant activity compared to conventional extraction procedures. The in vitro anti-neurodegenerative potential of extracts was measured through Acetylcholinesterase (AChE) inhibition assay. The results revealed the higher bioactivity observed in cowpea pod samples compared to seed extracts, which might be related to higher levels of quercetin and quercetin glycosides, kaempferol diglucoside, and other tetrahydroxylated flavones and flavonols identified in these samples. These results also provide an added-value benefit to the cultivation of this legume, considering the high potential of cowpea phenolic extracts as nutraceutical and functional ingredients in food formulations.
Overexpression of Transcription Factor GmTGA15 Enhances Drought Tolerance in Transgenic Soybean Hairy Roots and Arabidopsis Plants
Authors: Chen Zhanyu , Fang Xiaokun , Yuan Xueshun , Zhang Yingying , Li Huiying , Zhou Ying , Cui Xiyan
Published: January 2021

Soybean (Glycine max) is one of the important oil crops worldwide. In recent years, environmental stresses such as drought and soil salinization have severely deteriorated soybean yield and quality. We investigated the overexpression of the transcription factor GmTGA15 in response to drought stress in transgenic soybean hairy roots and Arabidopsis plants. The results of quantitative real time polymerase chain reaction (qRT-PCR) analyses showed that GmTGA15 was greatly induced by salt, PEG6000, salicylic acid (SA), gibberellic acid (GA), abscisic acid (ABA), and methyl jasmonate (MeJA) in soybean. In response to drought stress, the contents of both chlorophyll and proline were significantly increased, while the content of malondialdehyde (MDA) was significantly decreased in the soybean hairy roots with the overexpression of GmTGA15 in comparison to wild type (WT). Under the simulated drought conditions, the transgenic Arabidopsis plants showed significantly longer roots and lower mortality than that of the wild type. These results suggest that GmTGA15 promotes tolerance to drought stress in both soybean and Arabidopsis plants. This study provides the scientific evidence for further functional analysis of soybean TGA transcription factors in drought stress and the breeding of drought-resistance crops.
Fibre and Seed Productivity of Industrial Hemp (Cannabis sativa L.) Varieties under Mediterranean Conditions
Authors: Tsaliki Eleni , Kalivas Apostolos , Jankauskiene Zofija , Irakli Maria , Cook Catherine , Grigoriadis Ioannis , Panoras Ioannis , Vasilakoglou Ioannis , Dhima Kitsios
Published: January 2021

Farmers’ interest in renewable raw materials such as hemp (Cannabis sativa L.) fibres has recently increased, but hemp productivity is strongly affected by genotype and environment conditions. A 3-year field experiment was conducted under Mediterranean environment in northern Greece to evaluate the productivity (regarding fibres and seeds) of six monoecious hemp varieties. The vars. Futura 75 and Bialobrzeskie provided the greatest (p < 0.01) fibre productivity (4.57 and 4.27 t ha−1, respectively), which were 77.1% and 65.5%, respectively, greater than that of the least productive var. Fedora 17. However, the vars. Santhica 27, Tygra and Bialobrzeskie provided the highest (p < 0.05) seed yield (2.7, 2.9 and 2.6 t ha−1, respectively), which were 28.6%, 38.1% and 23.8%, respectively, greater than that of the least productive var. Futura 75. Hemp fibre yield was strongly positively correlated with total biomass (R2 = 0.8612) and stem biomass yield (R2 = 0.9742), while it was inversely correlated with fibre strength (R2 = 0.424). Hemp seed yield was not correlated with the hemp plant density, height, total biomass or stem biomass yield. The six hemp genotypes evaluated in the study had Δ9-tetrahydrocannabinol (THC) content lower than 0.2% satisfying the European legislation requirements for industrial hemp varieties. The results of the study indicated that, under Mediterranean conditions (northern Greece), the var. Bialobrzeskie showed high productivity, as averaged across years, for both fibres and seeds. This result is very helpful for farmers which should prefer hemp varieties of dual-purpose production (stems and inflorescences or stems and seeds) adapted best to their local environment.
Conservation and Use of Latin American Maize Diversity: Pillar of Nutrition Security and Cultural Heritage of Humanity
Authors: Guzzon Filippo , Arandia Rios Luis Walquer , Caviedes Cepeda Galo Mario , Céspedes Polo Marcia , Chavez Cabrera Alexander , Muriel Figueroa Jesús , Medina Hoyos Alicia Elizabeth , Jara Calvo Teófilo Wladimir , Molnar Terence L. , Narro León Luis Alberto , Narro León Teodoro Patricio , Mejía Kerguelén Sergio Luis , Ospina Rojas José Gabriel , Vázquez Gricelda , Preciado-Ortiz Ricardo Ernesto , Zambrano José Luis , Palacios Rojas Natalia , Pixley Kevin V.
Published: January 2021

Latin America is the center of domestication and diversity of maize, the second most cultivated crop worldwide. In this region, maize landraces are fundamental for food security, livelihoods, and culture. Nevertheless, genetic erosion (i.e., the loss of genetic diversity and variation in a crop) threatens the continued cultivation and in situ conservation of landrace diversity that is crucial to climate change adaptation and diverse uses of maize. We provide an overview of maize diversity in Latin America before discussing factors associated with persistence of large in situ maize diversity, causes for maize landrace abandonment by farmers, and strategies to enhance the cultivation of landraces. Among other factors, maize diversity is linked with: (1) small-holder farming, (2) the production of traditional food products, (3) traditional cropping systems, (4) cultivation in marginal areas, and (5) retention of control over the production system by the farmers. On the other hand, genetic erosion is associated with substitution of landraces with hybrid varieties or cash crops, and partial (off-farm labor) or complete migration to urban areas. Continued cultivation, and therefore on-farm conservation of genetic diversity held in maize landraces, can be encouraged by creating or strengthening market opportunities that make the cultivation of landraces and open pollinated varieties (OPVs) more profitable for farmers, supporting breeding programs that prioritize improvement of landraces and their special traits, and increasing the access to quality germplasm of landraces and landrace-derived OPVs.
Surface Defect Inspection in Images Using Statistical Patches Fusion and Deeply Learned Features
Authors: Eugene Chian Yan Tao , Tian Jing
Published: January 2021

Defect detection in images is a challenging task due to the existence of tiny and noisy patterns on surface images. To tackle this challenge, a defect detection approach is proposed in this paper using statistical data fusion. First, the proposed approach breaks a large image that contains multiple separate defects into smaller overlapping patches to detect the existence of defects in each patch, using the conventional convolutional neural network approach. Then, a statistical data fusion approach is proposed to maintain the spatial coherence of cracks in the image and aggregate the information extracted from overlapping patches to enhance the overall performance and robustness of the system. The proposed approach is evaluated using three benchmark datasets to demonstrate its superior performance in terms of both individual patch inspection and the whole image inspection.
Random Regression Analysis of Calving Interval of Japanese Black Cows
Authors: Ogawa Shinichiro , Satoh Masahiro
Published: January 2021

We estimated genetic parameters for the calving interval of Japanese Black cows using a random regression model and a repeatability model. We analyzed 92,019 calving interval records of 36,178 cows. Pedigree data covered 390,263 individuals. Age of cow at previous calving for each record ranged from 18 to 120 months. We used up to the second-order Legendre polynomials based on age at previous calving as sub-models for random regression analysis, and assumed a constant error variance across ages. Estimated heritability was 0.12 to 0.20 with the random regression model and 0.17 with the repeatability model. With the random regression model, the estimated genetic correlation between ages was ≥0.87, and those between 24 and 36 months, 24 and 84 months, and 36 and 84 months were 0.99, 0.95, and 0.97, respectively. Spearman’s rank correlation between breeding values of 36,178 cows with their own records estimated by the random regression model with those estimated using the repeatability model was ≥0.97, and the rank correlation was ≥0.94 for 314 sires of these cows. These results support the validity of fitting a repeatability model to the records of the calving interval of Japanese Black cows for evaluation of breeding values.
Sperm Cryopreservation in American Flamingo (Phoenicopterus Ruber): Influence of Cryoprotectants and Seminal Plasma Removal
Authors: Millán de la Blanca María Gemma , Martínez-Nevado Eva , Castaño Cristina , García Juncal , Bernal Berenice , Toledano-Díaz Adolfo , Esteso Milagros Cristina , Bóveda Paula , Martínez-Fresneda Lucía , López-Sebastián Antonio , Santiago-Moreno Julián
Published: January 2021

The American flamingo is a useful model for the development of successful semen cryopreservation procedures to be applied to threatened related species from the family Phoenicopteridae, and to permit genetic material banking. Current study sought to develop effective sperm cryopreservation protocols through examining the influences of two permeating cryoprotectants and the seminal plasma removal. During two consecutive years (April), semen samples were collected and frozen from American flamingos. In the first year, the effect of two permeating cryoprotectants, DMA (dimethylacetamide) (6%) or Me2SO (dimethylsulphoxide) (8%), on frozen–thawed sperm variables were compared in 21 males. No differences were seen between DMA and Me2SO for sperm motility, sperm viability, and DNA fragmentation after thawing. In the second year, the role of seminal plasma on sperm cryoresistance was investigated in 31 flamingos. Sperm samples were cryopreserved with and without seminal plasma, using Me2SO (8%) as a cryoprotectant. The results showed that samples with seminal plasma had higher values than samples without seminal plasma for the following sperm variables: Straight line velocity (22.40 µm/s vs. 16.64 µm/s), wobble (75.83% vs. 69.40%), (p < 0.05), linearity (62.73% vs. 52.01%) and straightness (82.38% vs. 73.79%) (p < 0.01); but acrosome integrity was lower (55.56% vs. 66.88%) (p < 0.05). The cryoresistance ratio (CR) was greater in samples frozen with seminal plasma than without seminal plasma for CR-progressive motility (138.72 vs. 54.59), CR-curvilinear velocity (105.98 vs. 89.32), CR-straight line velocity (152.77 vs. 112.58), CR-average path velocity (122.48 vs. 98.12), CR-wobble (111.75 vs. 102.04) (p < 0.05), CR-linearity (139.41 vs. 113.18), and CR-straightness (124.02 vs. 109.97) (p < 0.01). This research demonstrated that there were not differences between Me2SO and DMA to successful freezing sperm of flamingos; seminal plasma removal did not provide a benefit for sperm cryopreservation.
Resource Selection by Wild and Ranched White-Tailed Deer (Odocoileus virginianus) during the Epizootic Hemorrhagic Disease Virus (EHDV) Transmission Season in Florida
Authors: Dinh Emily T.N. , Orange Jeremy P. , Peters Rebecca M. , Wisely Samantha M. , Blackburn Jason K.
Published: January 2021

Epizootic hemorrhagic disease virus (EHDV) causes serious disease in wild and privately ranched white-tailed deer (Odocoileusvirginianus) in the United States. In Florida, there is high EHDV prevalence, yet no treatments. There are few management strategies for the disease due to limited knowledge of virus–vector–host interactions. We conducted a telemetry study on white-tailed deer to examine resource use by wild and ranched animals in the Florida panhandle during the 2016 transmission risk period. We built generalized linear mixed models (GLMMs) to estimate resource selection and map habitat preferences for wild and ranched deer in the study area to reveal how second-order selection may relate to higher disease prevalence in ranched deer. Wild deer preferred areas closer to tertiary roads and supplementary food sources but farther from permanent water. Ranched deer selected bottomland mixed forest and areas closer to tertiary roads, supplementary food sources, and permanent water. Ranched deer behaviors may increase the likelihood of EHDV vector encounters, as these deer selected preferred habitats of several putative vector species, which may increase vector blood meal success and viral transmission risk. Disparate resource selection behaviors may be a factor in observed differential EHDV exposure risk between ranched and wild white-tailed deer in Florida.
Novel Antioxidant, Deethylated Ethoxyquin, Protects against Carbon Tetrachloride Induced Hepatotoxicity in Rats by Inhibiting NLRP3 Inflammasome Activation and Apoptosis
Authors: Iskusnykh Igor Y. , Kryl’skii Evgenii D. , Brazhnikova Darya A. , Popova Tatyana N. , Shikhaliev Khidmet S. , Shulgin Konstantin K. , Matasova Larisa V. , Popov Sergey S. , Zhaglin Dmitry A. , Zakharova Anastasia A. , Popova Nelli R. , Fattakhov Nikolai
Published: January 2021

Inflammation and an increase in antioxidant responses mediated by oxidative stress play an important role in the pathogenesis of acute liver injury (ALI). We utilized in silico prediction of biological activity spectra for substances (PASS) analysis to estimate the potential biological activity profile of deethylated ethoxyquin (DEQ) and hypothesized that DEQ exhibits antioxidant and anti-inflammatory effects in a rat model of carbon tetrachloride (CCl4)-induced ALI. Our results demonstrate that DEQ improved liver function which was indicated by the reduction of histopathological liver changes. Treatment with DEQ reduced CCl4-induced elevation of gene expression, and the activity of antioxidant enzymes (AEs), as well as the expression of transcription factors Nfe2l2 and Nfkb2. Furthermore, DEQ treatment inhibited apoptosis, downregulated gene expression of pro-inflammatory cytokines (Tnf and Il6), cyclooxygenase 2 (Ptgs2), decreased glutathione (GSH) level and myeloperoxidase (MPO) activity in rats with ALI. Notably, DEQ treatment led to an inhibition of CCl4-induced NLRP3-inflammasome activation which was indicated by the reduced protein expression of IL-1β, caspase-1, and NLRP3 in the liver. Our data suggest that DEQ has a hepatoprotective effect mediated by redox-homeostasis regulation, NLRP3 inflammasome, and apoptosis inhibition, which makes that compound a promising candidate for future clinical studies.
Modulation of Autophagy in Cancer Cells by Dietary Polyphenols
Authors: Musial Claudia , Siedlecka-Kroplewska Kamila , Kmiec Zbigniew , Gorska-Ponikowska Magdalena
Published: January 2021

The role of autophagy is to degrade damaged or unnecessary cellular structures. Both in vivo and in vitro studies suggest a dual role of autophagy in cancer—it may promote the development of neoplasms, but it may also play a tumor protective function. The mechanism of autophagy depends on the genetic context, tumor stage and type, tumor microenvironment, or clinical therapy used. Autophagy also plays an important role in cell death as well as in the induction of chemoresistance of cancer cells. The following review describes the extensive autophagic cell death in relation to dietary polyphenols and cancer disease. The review documents increasing use of polyphenolic compounds in cancer prevention, or as agents supporting oncological treatment. Polyphenols are organic chemicals that exhibit antioxidant, anti-inflammatory, anti-angiogenic, and immunomodulating properties, and can also initiate the process of apoptosis. In addition, polyphenols reduce oxidative stress and protect against reactive oxygen species. This review presents in vitro and in vivo studies in animal models with the use of polyphenolic compounds such as epigallocatechin-3-gallate (EGCG), oleuropein, punicalgin, apigenin, resveratrol, pterostilbene, or curcumin and their importance in the modulation of autophagy-induced death of cancer cells.
The Multifaced Actions of Curcumin in Pregnancy Outcome
Authors: Tossetta Giovanni , Fantone Sonia , Giannubilo Stefano Raffaele , Marzioni Daniela
Published: January 2021

Curcumin, also known as diferuloylmethane, is the main polyphenolic substance present in the rhizomes of Curcuma longa L. This plant showed many beneficial effects and has been used since ancient times for both food and pharmaceutical purposes. Due to its pleiotropic functions, curcumin consumption in the human diet has become very common thanks also to the fact that this natural compound is considered quite safe as it does not have serious side effects. Its functions as an anti-inflammatory, anti-oxidant, neuroprotective, immunomodulatory, anti-toxicant, anti-apoptotic, and anti-diabetic compound are already known and widely demonstrated. There are numerous studies concerning its effects on various human pathologies including cancer, diabetes and arthritis while the studies on curcumin during pregnancy have been performed only in animal models. Data concerning the role of curcumin as anti-inflammatory compound suggest a possible use of curcumin in managing pregnancy complications such as Preeclampsia (PE), Gestational Diabetes Mellitus (GDM), Fetal Growth Restriction (FGR), PreTerm Birth (PTB), and exposure to toxic agents and pathogens. The aim of this review is to present data to support the possible use of curcumin in clinical trials on human gestation complications.
Research on Personalized Recommendation Methods for Online Video Learning Resources
Authors: Chen Xiaojuan , Deng Huiwen
Published: January 2021

It is not easy to find learning materials of interest quickly in the vast amount of online learning materials. The purpose of this study is to find students’ interests according to their learning behaviors in the network and to recommend related video learning materials. For the students who do not leave an evaluation record in the learning platform, the association rule algorithm in data mining is used to find out the videos that students are interested in and recommend them. For the students who have evaluation records in the platform, we use the collaborative filtering algorithm based on items in machine learning, and use the Pearson correlation coefficient method to find highly similar video materials, and then recommend the learning materials they are interested in. The two methods are used in different situations, and all students in the learning platform can get recommendation. Through the application, our methods can reduce the data search time, improve the stickiness of the platform, solve the problem of information overload, and meet the personalized needs of the learners.
Educational Warehouse: Modular, Private and Secure Cloudable Architecture System for Educational Data Storage, Analysis and Access
Authors: Amo Daniel , Gómez Pablo , Hernández-Ibáñez Luis , Fonseca David
Published: January 2021

Data in the educational context are becoming increasingly important in decision-making and teaching-learning processes. Similar to the industrial context, educational institutions are adopting data-processing technologies at all levels. To achieve representative results, the processes of extraction, transformation and uploading of educational data should be ubiquitous because, without useful data, either internal or external, it is difficult to perform a proper analysis and to obtain unbiased educational results. It should be noted that the source and type of data are heterogeneous and that the analytical processes can be so diverse that it opens up a practical problem of management and access to the data generated. At the same time, ensuring the privacy, identity, confidentiality and security of students and their data is a “sine qua non” condition for complying with the legal issues involved while achieving the required ethical premises. This work proposes a modular and scalable data system architecture that solves the complexity of data management and access. On the one hand, it allows educational institutions to collect any data generated in both the teaching-learning and management processes. On the other hand, it will enable external access to this data under appropriate privacy and security conditions.
An Immersive Virtual Reality Game for Predicting Risk Taking through the Use of Implicit Measures
Authors: de-Juan-Ripoll Carla , Llanes-Jurado José , Giglioli Irene Alice Chicchi , Marín-Morales Javier , Alcañiz Mariano
Published: January 2021

Risk taking (RT) measurement constitutes a challenge for researchers and practitioners and has been addressed from different perspectives. Personality traits and temperamental aspects such as sensation seeking and impulsivity influence the individual’s approach to RT, prompting risk-seeking or risk-aversion behaviors. Virtual reality has emerged as a suitable tool for RT measurement, since it enables the exposure of a person to realistic risks, allowing embodied interactions, the application of stealth assessment techniques and physiological real-time measurement. In this article, we present the assessment on decision making in risk environments (AEMIN) tool, as an enhanced version of the spheres and shield maze task, a previous tool developed by the authors. The main aim of this article is to study whether it is possible is to discriminate participants with high versus low scores in the measures of personality, sensation seeking and impulsivity, through their behaviors and physiological responses during playing AEMIN. Applying machine learning methods to the dataset we explored: (a) if through these data it is possible to discriminate between the two populations in each variable; and (b) which parameters better discriminate between the two populations in each variable. The results support the use of AEMIN as an ecological assessment tool to measure RT, since it brings to light behaviors that allow to classify the subjects into high/low risk-related psychological constructs. Regarding physiological measures, galvanic skin response seems to be less salient in prediction models.
Development of Inula graveolens (L.) Plant Extract Electrospun/Polycaprolactone Nanofibers: A Novel Material for Biomedical Application
Authors: Al-Kaabi Wasan J. , Albukhaty Salim , Al-Fartosy Adnan J. M. , Al-Karagoly Hassan Kh. , Al-Musawi Sharafaldin , Sulaiman Ghassan M. , Dewir Yaser H. , Alwahibi Mona S. , Soliman Dina A.
Published: January 2021

Recently, there has been a growing interest in research on nanofibrous scaffolds developed by electrospinning bioactive plant extracts. In this study, the extract material obtained from the medicinal plant Inula graveolens (L.) was loaded on polycaprolactone (PCL) electrospun polymeric nanofibers. The combined mixture was prepared by 5% of I. graveolens at 8% (PCL) concentration and electrospun under optimal conditions. The chemical analysis, morphology, and crystallization of polymeric nanofibers were carried out by (FT-IR) spectrometer, scanning electron microscopy (SEM), and XRD diffraction. Hydrophilicity was determined by a contact angle experiment. The strength was characterized, and the toxicity of scaffolds on the cell line of fibroblasts was finally investigated. The efficiency of nanofibers to enhance the proliferation of fibroblasts was evaluated in vitro using the optimal I. graveolens/PCL solutions. The results show that I. graveolens/PCL polymeric scaffolds exhibited dispersion in homogeneous nanofibers around 72 ± 963 nm in the ratio 70/30 (V:V), with no toxicity for cells, meaning that they can be used for biomedical applications.
Analysis and Experimental Verification of Mechanical Errors in Nine-Link Type Double-Toggle Mold/Die Clamping Mechanisms
Authors: Chang Wen-Tung , Lee Wei-I , Hsu Kuan-Lun
Published: January 2021

Nine-link type double-toggle mold/die clamping mechanisms are widely used in modern injection molding machines and die casting machines in order to provide sufficient mold/die clamping force for counteracting the pressure occurred inside molds/dies. In this paper, the analysis and experimental evaluation of mechanical errors in nine-link type double-toggle mold/die clamping mechanisms are presented. The kinematic error equations of the output link (i.e., the moving platen) caused by dimensional errors (or tolerances) of link members are derived analytically through the concept of tolerance sensitivity analysis. Evaluation indices based on the asymmetry of the mold/die clamping mechanism caused by mechanical errors are established. A case study is then given to demonstrate the derived analytical equations and the established evaluation indices. Subsequently, a prototype for performing the experimental evaluation is conceptually designed and was actually constructed. Experiments were conducted for evaluating the quantitative influence of mechanical errors on the operating performance of the constructed mold/die clamping mechanism. According to the experimental results, response surface modelling for benefiting the constructed mold/die clamping mechanism with better operating performance could be performed. The presented research results will be helpful in the tolerance analysis and mechanical error detection of nine-link type double-toggle mold/die clamping mechanisms.
Non-Thermal Methods for Ensuring the Microbiological Quality and Safety of Seafood
Authors: Ekonomou Sotirios I. , Boziaris Ioannis S.
Published: January 2021

A literature search and systematic review were conducted to present and discuss the most recent research studies for the past twenty years on the application of non-thermal methods for ensuring the microbiological safety and quality of fish and seafood. This review presents the principles and reveals the potential benefits of high hydrostatic pressure processing (HHP), ultrasounds (US), non-thermal atmospheric plasma (NTAP), pulsed electric fields (PEF), and electrolyzed water (EW) as alternative methods to conventional heat treatments. Some of these methods have already been adopted by the seafood industry, while others show promising results in inactivating microbial contaminants or spoilage bacteria from solid or liquid seafood products without affecting the biochemical or sensory quality. The main applications and mechanisms of action for each emerging technology are being discussed. Each of these technologies has a specific mode of microbial inactivation and a specific range of use. Thus, their knowledge is important to design a practical application plan focusing on producing safer, qualitative seafood products with added value following today’s consumers’ needs.
Surface Properties of Spray-Assisted Layer-By-Layer ElectroStatic Self-Assembly Treated Wooden Take-Off Board
Authors: Wan Yi , Hou Sijie , Guo Mengyao , Fu Yanchun
Published: January 2021

Wooden take-off board is easy to crack, deform, discolor, and decay when it is used outdoors, which not only increases maintenance costs but also reduces its service life. Multifunctional coatings with UV-resistant, water-repellent, and flame-retardant properties were successfully obtained on the surface of a wooden take-off board substrate by spray-assisted layer-by-layer self-assembly. The coatings consisted of positively-charged chitosan, Al (OH)3, and negatively-charged sodium phytate through electrostatic adsorption several times. The treated wood exhibited high UV resistance, and the color remained constant after 720 hours of ultraviolet irradiation. The wettability of the wood surface after treatment became superhydrophobic, with initial static contact angles as high as 140°. In addition, limiting oxygen index and air exposure combustion tests were used to verify that chitosan, sodium phytate, and aluminum hydroxide could synergistically confer significant fire resistance to modified wood.
Endemic Juniperus Montane Species Facing Extinction Risk under Climate Change in Southwest China: Integrative Approach for Conservation Assessment and Prioritization
Authors: Dakhil Mohammed , Halmy Marwa , Hassan Walaa , El-Keblawy Ali , Pan Kaiwen , Abdelaal Mohamed
Published: January 2021

Climate change is an important driver of biodiversity loss and extinction of endemic montane species. In China, three endemic Juniperus spp. (Juniperuspingii var. pingii, J.tibetica, and J.komarovii) are threatened and subjected to the risk of extinction. This study aimed to predict the potential distribution of these three Juniperus species under climate change and dispersal scenarios, to identify critical drivers explaining their potential distributions, to assess the extinction risk by estimating the loss percentage in their area of occupancy (AOO), and to identify priority areas for their conservation in China. We used ensemble modeling to evaluate the impact of climate change and project AOO. Our results revealed that the projected AOOs followed a similar trend in the three Juniperus species, which predicted an entire loss of their suitable habitats under both climate and dispersal scenarios. Temperature annual range and isothermality were the most critical key variables explaining the potential distribution of these three Juniperus species; they contribute by 16–56.1% and 20.4–38.3%, respectively. Accounting for the use of different thresholds provides a balanced approach for species distribution models’ applications in conservation assessment when the goal is to assess potential climatic suitability in new geographical areas. Therefore, south Sichuan and north Yunnan could be considered important priority conservation areas for in situ conservation and search for unknown populations of these three Juniperus species.
Effective Isolation for Lung Carcinoma Cells Based on Immunomagnetic Separation in a Microfluidic Channel
Authors: Vu-Dinh Hien , Feng Hui , Jen Chun-Ping
Published: January 2021

In this paper, we developed an isolation system for A549 human lung carcinoma cells as an effective factor for the early diagnosis of lung cancer. A microfluidic immunomagnetic method was used, in which the combination of immunomagnetic separation and a microfluidic system allowed for increased isolation efficiency with uncomplicated manipulation. In the microfluidic immunomagnetic strategy, A549 cells were combined with aptamer-conjugated carboxylated magnetic beads and then collected in a specified region by applying a magnetic field. The results were recorded using a fluorescence microscope, and the captured targets were then quantified. The isolation efficiency of A549 cells is up to 77.8%. This paper developed a simple working procedure, which is less time consuming, high-throughput, and trustworthy for the isolation of A549 cells. This procedure can be a useful reference method for the development of an effective diagnosis and treatment method for lung cancer in the future.
Influence of the Electrolyte Salt Concentration on DNA Detection with Graphene Transistors
Authors: Purwidyantri Agnes , Domingues Telma , Borme Jérôme , Guerreiro Joana Rafaela , Ipatov Andrey , Abreu Catarina M. , Martins Marco , Alpuim Pedro , Prado Marta
Published: January 2021

Liquid-gated Graphene Field-Effect Transistors (GFET) are ultrasensitive bio-detection platforms carrying out the graphene’s exceptional intrinsic functionalities. Buffer and dilution factor are prevalent strategies towards the optimum performance of the GFETs. However, beyond the Debye length (λD), the role of the graphene-electrolytes’ ionic species interactions on the DNA behavior at the nanoscale interface is complicated. We studied the characteristics of the GFETs under different ionic strength, pH, and electrolyte type, e.g., phosphate buffer (PB), and phosphate buffer saline (PBS), in an automatic portable built-in system. The electrostatic gating and charge transfer phenomena were inferred from the field-effect measurements of the Dirac point position in single-layer graphene (SLG) transistors transfer curves. Results denote that λD is not the main factor governing the effective nanoscale screening environment. We observed that the longer λD was not the determining characteristic for sensitivity increment and limit of detection (LoD) as demonstrated by different types and ionic strengths of measuring buffers. In the DNA hybridization study, our findings show the role of the additional salts present in PBS, as compared to PB, in increasing graphene electron mobility, electrostatic shielding, intermolecular forces and DNA adsorption kinetics leading to an improved sensitivity.
Modulation of Motor Cortex Plasticity by Repetitive Paired-Pulse TMS at Late I-Wave Intervals Is Influenced by Intracortical Excitability
Authors: Opie George M. , Sasaki Ryoki , Hand Brodie J. , Semmler John G.
Published: January 2021

The late indirect (I)-waves recruited by transcranial magnetic stimulation (TMS) over primary motor cortex (M1) can be modulated using I-wave periodicity repetitive TMS (iTMS). The purpose of this study was to determine if the response to iTMS is influenced by different interstimulus intervals (ISIs) targeting late I-waves, and whether these responses were associated with individual variations in intracortical excitability. Seventeen young (27.2 ± 6.4 years, 12 females) healthy adults received iTMS at late I-wave intervals (4.0, 4.5, and 5.0 ms) in three separate sessions. Changes due to each intervention were examined with motor evoked potential (MEP) amplitudes and short-interval intracortical facilitation (SICF) using both posterior-anterior (PA) and anterior-posterior (AP) TMS current directions. Changes in MEP amplitude and SICF were influenced by iTMS ISI, with the greatest facilitation for ISIs at 4 and 5 ms with PA TMS, and 4 ms with AP TMS. Maximum SICF at baseline (irrespective of ISI) was associated with increased iTMS response, but only for PA stimulation. These results suggest that modifying iTMS parameters targeting late I-waves can influence M1 plasticity. They also suggest that maximum SICF may be a means by which responders to iTMS targeting the late I-waves could be identified.
A High-Dimensional Window into the Micro-Environment of Triple Negative Breast Cancer
Authors: Nederlof Iris , Horlings Hugo M. , Curtis Christina , Kok Marleen
Published: January 2021

Providing effective personalized immunotherapy for triple negative breast cancer (TNBC) patients requires a detailed understanding of the composition of the tumor microenvironment. Both the tumor cell and non-tumor components of TNBC can exhibit tremendous heterogeneity in individual patients and change over time. Delineating cellular phenotypes and spatial topographies associated with distinct immunological states and the impact of chemotherapy will be necessary to optimally time immunotherapy. The clinical successes in immunotherapy have intensified research on the tumor microenvironment, aided by a plethora of high-dimensional technologies to define cellular phenotypes. These high-dimensional technologies include, but are not limited to, single cell RNA sequencing, spatial transcriptomics, T cell repertoire analyses, advanced flow cytometry, imaging mass cytometry, and their integration. In this review, we discuss the cellular phenotypes and spatial patterns of the lymphoid-, myeloid-, and stromal cells in the TNBC microenvironment and the potential value of mapping these features onto tumor cell genotypes.
Unraveling the Role of Innate Lymphoid Cells in Acute Myeloid Leukemia
Authors: Lordo Matthew R. , Scoville Steven D. , Goel Akul , Yu Jianhua , Freud Aharon G. , Caligiuri Michael A. , Mundy-Bosse Bethany L.
Published: January 2021

Over the past 50 years, few therapeutic advances have been made in treating acute myeloid leukemia (AML), an aggressive form of blood cancer, despite vast improvements in our ability to classify the disease. Emerging evidence suggests the immune system is important in controlling AML progression and in determining prognosis. Natural killer (NK) cells are important cytotoxic effector cells of the innate lymphoid cell (ILC) family that have been shown to have potent anti-leukemic functions. Recent studies are now revealing impairment or dysregulation of other ILCs in various types of cancers, including AML, which limits the effectiveness of NK cells in controlling cancer progression. NK cell development and function are inhibited in AML patients, which results in worse clinical outcomes; however, the specific roles of other ILC populations in AML are just now beginning to be unraveled. In this review, we summarize what is known about the role of ILC populations in AML.