bluuebunny/crossref_metadata_2025 huggingface dataset:
Through Reducing ROS Production, IL-10 Suppresses Caspase-1-Dependent IL-1β Maturation, thereby Preventing Chronic Neuroinflammation and Neurodegeneration
Authors: Gao Yun , Tu Dezhen , Yang Ru , Chu Chun-Hsien , Hong Jau-Shyong , Gao Hui-Ming
Published: January 2020

Chronic neuroinflammation contributes to the pathogenesis of Parkinson’s disease (PD). However, cellular and molecular mechanisms by which chronic neuroinflammation is formed and maintained remain elusive. This study aimed to explore detailed mechanisms by which anti-inflammatory cytokine interleukin-10 (IL-10) prevented chronic neuroinflammation and neurodegeneration. At 24 h after an intranigral injection of lipopolysaccharide (LPS), levels of NLRP3, pro-caspase-1, pro-IL-1β, active caspase-1, and mature IL-1β in the midbrain were much higher in IL-10−/− mice than wildtype mice. Mechanistically, IL-10−/− microglia produced more intracellular reactive oxygen species (iROS) and showed more profound activation of NADPH oxidase (NOX2) than wildtype microglia. Meanwhile, suppression of NOX2-derived iROS production blocked LPS-elicited caspase-1 activation and IL-1β maturation in IL-10−/− microglia in vitro and in vivo. One month after intranigral LPS injection, IL-10−/− mice revealed more profound microglial activation and dopaminergic neurodegeneration in the substantia nigra than wildtype mice. Importantly, such PD-like pathological changes were prevented by IL-1β neutralization. Collectively, IL-10 inhibited LPS-elicited production of NOX2-derived iROS thereby suppressing synthesis of NLRP3, pro-caspase-1 and pro-IL-1β and their activation and cleavage. By this mechanism, IL-10 prevented chronic neuroinflammation and neurodegeneration. This study suggested boosting anti-inflammatory effects of IL-10 and suppressing NLRP3 inflammasome activation could be beneficial for PD treatment.
Epimers of Vitamin D: A Review
Authors: Al-Zohily Bashar , Al-Menhali Asma , Gariballa Salah , Haq Afrozul , Shah Iltaf
Published: January 2020

In this review, we discuss the sources, formation, metabolism, function, biological activity, and potency of C3-epimers (epimers of vitamin D). We also determine the role of epimerase in vitamin D-binding protein (DBP) and vitamin D receptors (VDR) according to different subcellular localizations. The importance of C3 epimerization and the metabolic pathway of vitamin D at the hydroxyl group have recently been recognized. Here, the hydroxyl group at the C3 position is orientated differently from the alpha to beta orientation in space. However, the details of this epimerization pathway are not yet clearly understood. Even the gene encoding for the enzyme involved in epimerization has not yet been identified. Many published research articles have illustrated the biological activity of C3 epimeric metabolites using an in vitro model, but the studies on in vivo models are substantially inadequate. The metabolic stability of 3-epi-1α,25(OH)2D3 has been demonstrated to be higher than its primary metabolites. 3-epi-1 alpha, 25 dihydroxyvitamin D3 (3-epi-1α,25(OH)2D3) is thought to have fewer calcemic effects than non-epimeric forms of vitamin D. Some researchers have observed a larger proportion of total vitamin D as C3-epimers in infants than in adults. Insufficient levels of vitamin D were found in mothers and their newborns when the epimers were not included in the measurement of vitamin D. Oral supplementation of vitamin D has also been found to potentially cause increased production of epimers in mice but not humans. Moreover, routine vitamin D blood tests for healthy adults will not be significantly affected by epimeric interference using LC–MS/MS assays. Recent genetic models also show that the genetic determinants and the potential factors of C3-epimers differ from those of non-C3-epimers.Most commercial immunoassays techniques can lead to inaccurate vitamin D results due to epimeric interference, especially in infants and pregnant women. It is also known that the LC–MS/MS technique can chromatographically separate epimeric and isobaric interference and detect vitamin D metabolites sensitively and accurately. Unfortunately, many labs around the world do not take into account the interference caused by epimers. In this review, various methods and techniques for the analysis of C3-epimers are also discussed. The authors believe that C3-epimers may have an important role to play in clinical research, and further research is warranted.
Tumor-Stroma Crosstalk Enhances REG3A Expressions that Drive the Progression of Hepatocellular Carcinoma
Authors: Cho Yuri , Park Min Ji , Kim Koeun , Park Jae-Young , Kim Jihye , Kim Wonjin , Yoon Jung-Hwan
Published: January 2020

Abstract: Background: Crosstalk between tumors and their microenvironment plays a crucial role in the progression of hepatocellular carcinoma (HCC). However, there is little existing information about the key signaling molecule that modulates tumor-stroma crosstalk. Methods: Complementary DNA (cDNA) microarray analysis was performed to identify the key molecule in tumor-stroma crosstalk. Subcutaneous xenograft in vivo murine model, immunoblotting, immunofluorescence, and real-time polymerase chain reaction using HCC cells and tissues were performed. Results: The key molecule, regenerating gene protein-3A (REG3A), was most significantly enhanced when coculturing HCC cells and activated human hepatic stellate cells (HSCs) (+8.2 log) compared with monoculturing HCC cells using cDNA microarray analysis. Downregulation of REG3A using small interfering RNA significantly decreased the proliferation of HSC-cocultured HCC cells in vitro and in vivo, and enhanced deoxycholic acid-induced HCC cell apoptosis. Crosstalk-induced REG3A upregulation was modulated by platelet-derived growth factor ββ (PDGF-ββ) in p42/44-dependent manner. REG3A mRNA levels in human HCC tissues were upregulated 1.8-fold compared with non-tumor tissues and positively correlated with PDGF-ββ levels. Conclusions: REG3A/p42/44 pathway/PDGF-ββ signaling plays a significant role in hepatocarcinogenesis via tumor-stroma crosstalk. Targeting REG3A is a potential novel therapeutic target for the management of HCCs by inhibiting crosstalk between HCC cells and HSCs.
A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress
Authors: Omidbakhshfard Mohammad Amin , Sujeeth Neerakkal , Gupta Saurabh , Omranian Nooshin , Guinan Kieran J. , Brotman Yariv , Nikoloski Zoran , Fernie Alisdair R. , Mueller-Roeber Bernd , Gechev Tsanko S.
Published: January 2020

Abiotic stresses cause oxidative damage in plants. Here, we demonstrate that foliar application of an extract from the seaweed Ascophyllum nodosum, SuperFifty (SF), largely prevents paraquat (PQ)-induced oxidative stress in Arabidopsis thaliana. While PQ-stressed plants develop necrotic lesions, plants pre-treated with SF (i.e., primed plants) were unaffected by PQ. Transcriptome analysis revealed induction of reactive oxygen species (ROS) marker genes, genes involved in ROS-induced programmed cell death, and autophagy-related genes after PQ treatment. These changes did not occur in PQ-stressed plants primed with SF. In contrast, upregulation of several carbohydrate metabolism genes, growth, and hormone signaling as well as antioxidant-related genes were specific to SF-primed plants. Metabolomic analyses revealed accumulation of the stress-protective metabolite maltose and the tricarboxylic acid cycle intermediates fumarate and malate in SF-primed plants. Lipidome analysis indicated that those lipids associated with oxidative stress-induced cell death and chloroplast degradation, such as triacylglycerols (TAGs), declined upon SF priming. Our study demonstrated that SF confers tolerance to PQ-induced oxidative stress in A. thaliana, an effect achieved by modulating a range of processes at the transcriptomic, metabolic, and lipid levels.
Impaired Glycolysis Promotes Alcohol Exposure-Induced Apoptosis in HEI-OC1 Cells via Inhibition of EGFR Signaling
Authors: Kang Hyunsook , Choi Seong Jun , Park Kye Hoon , Lee Chi-Kyou , Moon Jong-Seok
Published: January 2020

Glucose metabolism is an important metabolic pathway in the auditory system. Chronic alcohol exposure can cause metabolic dysfunction in auditory cells during hearing loss. While alcohol exposure has been linked to hearing loss, the mechanism by which impaired glycolysis promotes cytotoxicity and cell death in auditory cells remains unclear. Here, we show that the inhibition of epidermal growth factor receptor (EGFR)-induced glycolysis is a critical mechanism for alcohol exposure-induced apoptosis in HEI-OC1 cells. The cytotoxicity via apoptosis was significantly increased by alcohol exposure in HEI-OC1 cells. The glycolytic activity and the levels of hexokinase 1 (HK1) were significantly suppressed by alcohol exposure in HEI-OC1 cells. Mechanistic studies showed that the levels of EGFR and AKT phosphorylation were reduced by alcohol exposure in HEI-OC1 cells. Notably, HK1 expression and glycolytic activity was suppressed by EGFR inhibition in HEI-OC1 cells. These results suggest that impaired glycolysis promotes alcohol exposure-induced apoptosis in HEI-OC1 cells via the inhibition of EGFR signaling.
Blood Co-Circulating Extracellular microRNAs and Immune Cell Subsets Associate with Type 1 Diabetes Severity
Authors: Garavelli Silvia , Bruzzaniti Sara , Tagliabue Elena , Prattichizzo Francesco , Di Silvestre Dario , Perna Francesco , La Sala Lucia , Ceriello Antonio , Mozzillo Enza , Fattorusso Valentina , Mauri Pierluigi , Puca Annibale A. , Franzese Adriana , Matarese Giuseppe , Galgani Mario , de Candia Paola
Published: January 2020

Immune cell subsets and microRNAs have been independently proposed as type 1 diabetes (T1D) diagnostic and/or prognostic biomarkers. Here, we aimed to analyze the relationships between peripheral blood circulating immune cell subsets, plasmatic microRNAs, and T1D. Blood samples were obtained from both children with T1D at diagnosis and age-sex matched healthy controls. Then, immunophenotype assessed by flow cytometry was coupled with the quantification of 60 plasmatic microRNAs by quantitative RT-PCR. The associations between immune cell frequency, plasmatic microRNAs, and the parameters of pancreatic loss, glycemic control, and diabetic ketoacidosis were assessed by logistic regression models and correlation analyses. We found that the increase in specific plasmatic microRNAs was associated with T1D disease onset (let-7c-5p, let-7d-5p, let-7f-5p, let-7i-5p, miR-146a-5p, miR-423-3p, and miR-423-5p), serum C-peptide concentration (miR-142-5p and miR-29c-3p), glycated hemoglobin (miR-26a-5p and miR-223-3p) and the presence of ketoacidosis (miR-29c-3p) more strongly than the evaluated immune cell subset frequency. Some of these plasmatic microRNAs were shown to positively correlate with numbers of blood circulating B lymphocytes (miR-142-5p) and CD4+CD45RO+ (miR-146a-5p and miR-223-3p) and CD4+CD25+ cells (miR-423-3p and miR-223-3p) in children with T1D but not in healthy controls, suggesting a disease-specific microRNA association with immune dysregulation in T1D. In conclusion, our results suggest that, while blood co-circulating extracellular microRNAs and immune cell subsets may be biologically linked, microRNAs may better provide powerful information about T1D onset and severity.
Genome-Wide Mapping of Quantitative Trait Loci Conferring All-Stage and High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat Landrace PI 181410
Authors: Liu Yan , Qie Yanmin , Li Xing , Wang Meinan , Chen Xianming
Published: January 2020

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat in the world. Genetic resistance is the best strategy for control of the disease. Spring wheat landrace PI 181410 has shown high level resistance to stripe rust. The present study characterized the landrace to have both race-specific all-stage resistance and nonrace-specific high-temperature adult-plant (HTAP) resistance. To map quantitative trait loci (QTL) for the resistance in PI 181410, it was crossed with Avocet S (AvS), from which a recombinant inbred line population was developed. The F5–F8 populations were consecutively phenotyped for stripe rust response in multiple field environments under natural Pst infection, and the F7 population was phenotyped in seedlings at low temperature and in adult-plant stage with selected Pst races in the greenhouse. The F7 population was genotyped using the 90K wheat SNP chip. Three QTL, QYrPI181410.wgp-4AS, QYrPI181410.wgp-4BL, and QYrPI181410.wgp-5BL.1, from PI 181410 for all-stage resistance, were mapped on chromosome arms 4AS, 4BL, and 5BL, respectively. Four QTL, QYrPI181410.wgp-1BL, QYrPI181410.wgp-4BL, QYrPI181410.wgp-5AS, and QYrPI181410.wgp-5BL.2, were identified from PI 181410 for HTAP resistance and mapped to 1BL, 4BL, 5AS, and 5BL, respectively. Two QTL with minor effects on stripe rust response were identified from AvS and mapped to 2BS and 2BL. Four of the QTL from PI 181410 and one from AvS were potentially new. As the 4BL QTL was most effective and likely a new gene for stripe rust resistance, three kompetitive allele specific PCR (KASP) markers were developed for incorporating this gene into new wheat cultivars.
Current and Alternative Therapies for Nasal Mucosa Injury: A Review
Authors: Selvarajah Jegadevswari , Saim Aminuddin Bin , Bt Hj Idrus Ruszymah , Lokanathan Yogeswaran
Published: January 2020

Nasal mucosa injury can be caused by trauma, radiotherapy, chronic infection such as sinusitis, and post sinus surgery. The rate of healing and its treatment are important in the recovery of patients especially in post sinus surgery, which introduces new injuries. In this review, the current knowledge in terms of the mechanism underlying nasal wound healing was initially discussed. The currently available treatment options for enhancement of wound healing following sinus surgery were discussed and these had included intravenous antibiotics or steroids, various nasal sprays, and nasal packing. In addition, emerging alternative therapies in nasal mucosa wound healing such as herbal medicine and the advancement of regenerative medicine therapies such as stem cells and their byproducts were also discussed. Despite the various available treatment options for wound healing in nasal mucosa, rigorous strong evidence of their efficacy is gravely warranted in order to recommend them as part of the treatment modality.
Progress of Induced Pluripotent Stem Cell Technologies to Understand Genetic Epilepsy
Authors: Sterlini Bruno , Fruscione Floriana , Baldassari Simona , Benfenati Fabio , Zara Federico , Corradi Anna
Published: January 2020

The study of the pathomechanisms by which gene mutations lead to neurological diseases has benefit from several cellular and animal models. Recently, induced Pluripotent Stem Cell (iPSC) technologies have made possible the access to human neurons to study nervous system disease-related mechanisms, and are at the forefront of the research into neurological diseases. In this review, we will focalize upon genetic epilepsy, and summarize the most recent studies in which iPSC-based technologies were used to gain insight on the molecular bases of epilepsies. Moreover, we discuss the latest advancements in epilepsy cell modeling. At the two dimensional (2D) level, single-cell models of iPSC-derived neurons lead to a mature neuronal phenotype, and now allow a reliable investigation of synaptic transmission and plasticity. In addition, functional characterization of cerebral organoids enlightens neuronal network dynamics in a three-dimensional (3D) structure. Finally, we discuss the use of iPSCs as the cutting-edge technology for cell therapy in epilepsy.
Idebenone Protects against Acute Murine Colitis via Antioxidant and Anti-Inflammatory Mechanisms
Authors: Shastri Sonia , Shinde Tanvi , Sohal Sukhwinder Singh , Gueven Nuri , Eri Rajaraman
Published: January 2020

Oxidative stress is a key player of the inflammatory cascade responsible for the initiation of ulcerative colitis (UC). Although the short chain quinone idebenone is considered a potent antioxidant and a mitochondrial electron donor, emerging evidence suggests that idebenone also displays anti-inflammatory activity. This study evaluated the impact of idebenone in the widely used dextran sodium sulphate (DSS)-induced mouse model of acute colitis. Acute colitis was induced in C57BL/6J mice via continuous exposure to 2.5% DSS over 7 days. Idebenone was co-administered orally at a dose of 200 mg/kg body weight. Idebenone significantly prevented body weight loss and improved the disease activity index (DAI), colon length, and histopathological score. Consistent with its reported antioxidant function, idebenone significantly reduced the colonic levels of malondialdehyde (MDA) and nitric oxide (NO), and increased the expression of the redox factor NAD(P)H (nicotinamide adenine dinucleotide phosphate) dehydrogenase quinone-1 (NQO-1) in DSS-exposed mice. Immunohistochemistry revealed a significantly increased expression of tight junction proteins, which protect and maintain paracellular intestinal permeability. In support of an anti-inflammatory activity, idebenone significantly attenuated the elevated levels of pro-inflammatory cytokines in colon tissue. These results suggest that idebenone could represent a promising therapeutic strategy to interfere with disease pathology in UC by simultaneously inducing antioxidative and anti-inflammatory pathways.
Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions
Authors: Zhong Zhuoheng , Furuya Takashi , Ueno Kimitaka , Yamaguchi Hisateru , Hitachi Keisuke , Tsuchida Kunihiro , Tani Masahiko , Tian Jingkui , Komatsu Setsuko
Published: January 2020

Improving soybean growth and tolerance under environmental stress is crucial for sustainable development. Millimeter waves are a radio-frequency band with a wavelength range of 1–10 mm that has dynamic effects on organisms. To investigate the potential effects of millimeter-waves irradiation on soybean seedlings, morphological and proteomic analyses were performed. Millimeter-waves irradiation improved the growth of roots/hypocotyl and the tolerance of soybean to flooding stress. Proteomic analysis indicated that the irradiated soybean seedlings recovered under oxidative stress during growth, whereas proteins related to glycolysis and ascorbate/glutathione metabolism were not affected. Immunoblot analysis confirmed the promotive effect of millimeter waves to glycolysis- and redox-related pathways under flooding conditions. Sugar metabolism was suppressed under flooding in unirradiated soybean seedlings, whereas it was activated in the irradiated ones, especially trehalose synthesis. These results suggest that millimeter-waves irradiation on soybean seeds promotes the recovery of soybean seedlings under oxidative stress, which positively regulates soybean growth through the regulation of glycolysis and redox related pathways.
Combined Targeting of Mutant p53 and Jumonji Family Histone Demethylase Augments Therapeutic Efficacy of Radiation in H3K27M DIPG
Authors: Nikolaev Anatoly , Fiveash John B. , Yang Eddy S.
Published: January 2020

Diffuse intrinsic pontine glioma (DIPG) is an aggressive pediatric brainstem tumor with a 5-year survival of <1%. Up to 80% of the DIPG tumors contain a specific K27M mutation in one of the two genes encoding histone H3 (H3K27M). Furthermore, p53 mutations found in >70–80% of H3K27M DIPG, and mutant p53 status is associated with a decreased response to radiation treatment and worse overall prognosis. Recent evidence indicates that H3K27M mutation disrupts tri-methylation at H3K27 leading to aberrant gene expression. Jumonji family histone demethylases collaborates with H3K27 mutation in DIPG by erasing H3K27 trimethylation and thus contributing to derepression of genes involved in tumorigenesis. Since the first line of treatment for pediatric DIPG is fractionated radiation, we investigated the effects of Jumonji demethylase inhibition with GSK-J4, and mutant p53 targeting/oxidative stress induction with APR-246, on radio-sensitization of human H3K27M DIPG cells. Both APR-246 and GSK-J4 displayed growth inhibitory effects as single agents in H3K27M DIPG cells. Furthermore, both of these agents elicited mild radiosensitizing effects in human DIPG cells (sensitizer enhancement ratios (SERs) of 1.12 and 1.35, respectively; p < 0.05). Strikingly, a combination of APR-246 and GSK-J4 displayed a significant enhancement of radiosensitization, with SER of 1.50 (p < 0.05) at sub-micro-molar concentrations of the drugs (0.5 μM). The molecular mechanism of the observed radiosensitization appears to involve DNA damage repair deficiency triggered by APR-246/GSK-J4, leading to the induction of apoptotic cell death. Thus, a therapeutic approach of combined targeting of mutant p53, oxidative stress induction, and Jumonji demethylase inhibition with radiation in DIPG warrants further investigation.
Quercetin in Animal Models of Alzheimer’s Disease: A Systematic Review of Preclinical Studies
Authors: Zhang Xiao-Wen , Chen Jia-Yue , Ouyang Defang , Lu Jia-Hong
Published: January 2020

Alzheimer’s disease (AD) is the leading cause of dementia worldwide. It involves progressive impairment of cognitive function. A growing number of neuroprotective compounds have been identified with potential anti-AD properties through in vitro and in vivo models of AD. Quercetin, a natural flavonoid contained in a wide range of plant species, is repeatedly reported to exert neuroprotective effects in experimental animal AD models. However, a systematic analysis of methodological rigor and the comparison between different studies is still lacking. A systematic review uses a methodical approach to minimize the bias in each independent study, providing a less biased, comprehensive understanding of research findings and an objective judgement of the strength of evidence and the reliability of conclusions. In this review, we identified 14 studies describing the therapeutic efficacy of quercetin on animal AD models by electronic and manual retrieval. Some of the results of the studies included were meta-analyzed by forest plot, and the methodological quality of each preclinical trial was assessed with SYRCLE’s risk of bias tool. Our results demonstrated the consistent neuroprotective effects of quercetin on different AD models, and the pharmacological mechanisms of quercetin on AD models are summarized. This information eliminated the bias of each individual study, providing guidance for future tests and supporting evidence for further implementation of quercetin into clinical trials. However, the limitations of some studies, such as the absence of sample size calculations and low method quality, should also be noted.
Comparing Web Accessibility Evaluation Tools and Evaluating the Accessibility of Webpages: Proposed Frameworks
Authors: Alsaeedi Abdullah
Published: January 2020

With the growth of e-services in the past two decades, the concept of web accessibility has been given attention to ensure that every individual can benefit from these services without any barriers. Web accessibility is considered one of the main factors that should be taken into consideration while developing webpages. Web Content Accessibility Guidelines 2.0 (WCAG 2.0) have been developed to guide web developers to ensure that web contents are accessible for all users, especially disabled users. Many automatic tools have been developed to check the compliance of websites with accessibility guidelines such as WCAG 2.0 and to help web developers and content creators with designing webpages without barriers for disabled people. Despite the popularity of accessibility evaluation tools in practice, there is no systematic way to compare the performance of web accessibility evaluators. This paper first presents two novel frameworks. The first one is proposed to compare the performance of web accessibility evaluation tools in detecting web accessibility issues based on WCAG 2.0. The second framework is utilized to evaluate webpages in meeting these guidelines. Six homepages of Saudi universities were chosen as case studies to substantiate the concept of the proposed frameworks. Furthermore, two popular web accessibility evaluators, Wave and SiteImprove, are selected to compare their performance. The outcomes of studies conducted using the first proposed framework showed that SiteImprove outperformed WAVE. According to the outcomes of the studies conducted, we can conclude that web administrators would benefit from the first framework in selecting an appropriate tool based on its performance to evaluate their websites based on accessibility criteria and guidelines. Moreover, the findings of the studies conducted using the second proposed framework showed that the homepage of Taibah University is more accessible than the homepages of other Saudi universities. Based on the findings of this study, the second framework can be used by web administrators and developers to measure the accessibility of their websites. This paper also discusses the most common accessibility issues reported by WAVE and SiteImprove.
Jejunal Insulin Signalling Is Increased in Morbidly Obese Subjects with High Insulin Resistance and Is Regulated by Insulin and Leptin
Authors: Gutierrez-Repiso Carolina , Ho-Plagaro Ailec , Santiago-Fernandez Concepción , Garcia-Serrano Sara , Rodríguez-Pacheco Francisca , Valdes Sergio , Garrido-Sanchez Lourdes , Rodríguez-Díaz Cristina , López-Gómez Carlos , Moreno-Ruiz Francisco J. , Alcain-Martinez Guillermo , Gautier-Stein Amandine , Mithieux Gilles , Garcia-Fuentes Eduardo
Published: January 2020

Little is known about the jejunal insulin signalling pathways in insulin resistance/diabetes states and their possible regulation by insulin/leptin. We study in jejunum the relation between insulin signalling and insulin resistance in morbidly obese subjects with low (MO-low-IR) or with high insulin resistance (MO-high-IR), and with type 2 diabetes treated with metformin (MO-metf-T2DM), and the effect of insulin/leptin on intestinal epithelial cells (IEC). Insulin receptor substrate-1 (IRS1) and the catalytic p110β subunit (p110β) of phosphatidylinositol 3-kinase (PI3K) were higher in MO-high-IR than in MO-low-IR. The regulatory p85α subunit of PI3K (p85α)/p110β ratio was lower in MO-high-IR and MO-metf-T2DM than in MO-low-IR. Akt-phosphorylation in Ser473 was reduced in MO-high-IR compared with MO-low-IR. IRS1 and p110-β were associated with insulin and leptin levels. The improvement of body mass index (BMI) and HOMA-IR (homeostasis model assessment of insulin resistance index) after bariatric surgery was associated with a higher IRS1 and a lower p85α/p110β ratio. IEC (intestinal epithelial cells) incubation with a high glucose + insulin dose produced an increase of p85α and p110β. High dose of leptin produced an increase of IRS1, p85α and p110β. In conclusion, despite the existence of insulin resistance, the jejunal expression of genes involved in insulin signalling was increased in MO-high-IR. Their expressions were regulated mainly by leptin. IRS1 and p85α/p110β ratio was associated with the evolution of insulin resistance after bariatric surgery.
Efficacy of Different Chemotherapy Regimens in Patients with Locally Advanced Synchronous Esophageal and Head/Neck Squamous Cell Carcinoma Receiving Curative Concurrent Chemoradiotherapy
Authors: Chen Yen-Hao , Lu Hung-I , Chien Chih-Yen , Lo Chien-Ming , Wang Yu-Ming , Chou Shang-Yu , Li Shau-Hsuan
Published: January 2020

Esophageal squamous cell carcinoma (ESCC) and head/neck squamous cell carcinoma (HNSCC) are very common cancers worldwide, and there is higher incidence of synchronous ESCC/NSCC in Taiwan. The aim of the current study was to investigate the efficacy of different chemotherapy regimens in patients with locally advanced synchronous ESCC/HNSCC who received curative concurrent chemoradiotheapy (CCRT). A total of 75 patients were identified and assigned to one of two groups: 45 patients receiving cisplatin/5-fluorouracil (5-FU) regime in one group and 30 patients receiving a weekly cisplatin regime in the other. Overall survival (OS) was calculated from the date of diagnosis of the ESCC or HNSCC to the date of death from any cause or the most recent follow-up. Kaplan–Meier curves and log-rank tests were used to estimate OS and differences between the two groups, respectively. There was no significant difference in the analysis of OS between the cisplatin/5-FU and the weekly cisplatin groups. However, patients that interrupted their CCRT were found to have worse OS compared to those without interruptions (5.4 months versus 18.8 months, p = 0.002). In subgroup analysis, patients without interruptions of CCRT had a better OS than those with interruptions in the cisplatin/5-FU group (13.0 months versus 5.4 months, p = 0.041) as well as in the weekly cisplatin group (21.4 months versus 5.0 months, p = 0.017). Interruption of CCRT was the only independently poor prognostic factor of OS in the univariate and multivariate (hazard ratio 0.18, p < 0.001) analyses. Most interruption of CCRT resulted from adverse events (AEs) or serious AEs. Although there was no significant difference in the incidence of AEs between these two groups, lower incidence of adverse events was mentioned in the weekly cisplatin group. Our study suggests that interruption of CCRT is an independently poor prognostic factor of OS, and that completion of CCRT without interruption is more important than the choice of chemotherapeutic regimen for patients with synchronous ESCC/HNSCC.
The Predictive Value of Low Muscle Mass as Measured on CT Scans for Postoperative Complications and Mortality in Gastric Cancer Patients: A Systematic Review and Meta-Analysis
Authors: Borggreve Alicia S. , den Boer Robin B. , van Boxel Gijs I. , de Jong Pim A. , Veldhuis Wouter B. , Steenhagen Elles , van Hillegersberg Richard , Ruurda Jelle P.
Published: January 2020

Risk assessment is relevant to predict outcomes in patients with gastric cancer. This systematic review aimed to investigate the predictive value of low muscle mass for postoperative complications in gastric cancer patients. A systematic literature search was performed to identify all articles reporting on muscle mass as measured on computed tomography (CT) scans in patients with gastric cancer. After full text screening, 15 articles reporting on 4887 patients were included. Meta-analysis demonstrated that patients with low muscle mass had significantly higher odds of postoperative complications (odds ratio (OR): 2.09, 95% confidence interval (CI): 1.55–2.83) and severe postoperative complications (Clavien–Dindo grade ≥III, OR: 1.73, 95% CI: 1.14–2.63). Moreover, patients with low muscle mass had a significantly higher overall mortality (hazard ratio (HR): 1.81, 95% CI: 1.52–2.14) and disease-specific mortality (HR: 1.58, 95% CI: 1.36–1.84). In conclusion, assessment of muscle mass on CT scans is a potential relevant clinical tool for risk prediction in gastric cancer patients. Considering the heterogeneity in definitions applied for low muscle mass on CT scans in the included studies, a universal cutoff value of CT-based low muscle mass is required for more reliable conclusions.
Does Beta-Trace Protein (BTP) Outperform Cystatin C as a Diagnostic Marker of Acute Kidney Injury Complicating the Early Phase of Acute Pancreatitis?
Authors: Wajda Justyna , Dumnicka Paulina , Sporek Mateusz , Maziarz Barbara , Kolber Witold , Ząbek-Adamska Anna , Ceranowicz Piotr , Kuźniewski Marek , Kuśnierz-Cabala Beata
Published: January 2020

Acute pancreatitis (AP) belongs to the commonest acute gastrointestinal conditions requiring hospitalization. Acute kidney injury (AKI) often complicates moderately severe and severe AP, leading to increased mortality. Among the laboratory markers proposed for early diagnosis of AKI, few have been studied in AP, including cystatin C and neutrophil gelatinase-associated lipocalin (NGAL). Beta-trace protein (BTP), a low-molecular-weight glycoprotein proposed as an early marker of decreased glomerular filtration, has never been studied in AP. We investigated the diagnostic usefulness of serum BTP for early diagnosis of AKI complicating AP in comparison to previously studied markers. BTP was measured in serum samples collected over the first three days of hospital stay from 73 adult patients admitted within 24 h of mild to severe AP. Thirteen patients (18%) developed AKI in the early phase of AP. Serum BTP was higher in patients who developed AKI, starting from the first day of hospitalization. Strong correlations were observed between BTP and serum cystatin C but not serum or urine NGAL. On admission, BTP positively correlated with endothelial dysfunction. The diagnostic usefulness of BTP for AKI was similar to cystatin C and lower than NGAL. Increased BTP is an early predictor of AKI complicating AP. However, it does not outperform cystatin C or NGAL.
The Effectiveness of Psychological Treatments in Women with Breast Cancer: A Systematic Review and Meta-Analysis
Authors: Guarino Angela , Polini Cristina , Forte Giuseppe , Favieri Francesca , Boncompagni Ilaria , Casagrande Maria
Published: January 2020

Breast cancer is the most prevalent oncological disease among women, and it represents the second oncological cause of death. Many studies have considered the quality of life in people with breast cancer because this condition has high comorbidity with mental distress, anxiety, affective disorders and depression. Psychological interventions can reduce the stressful consequences of both the diagnosis and the medical treatments of breast cancer. However, different methods (e.g., group or individual therapy) and focus (e.g., improving personal skills or increasing emotional well-being) do not help to identify which type of psychological therapy can be more effective in improving quality of life in patients with breast cancer. This study was aimed to systematically review and compare, by means of a meta-analysis, the efficacy of cognitive behavioural, supportive-expressive or psycho-educational treatments in women with breast cancer, focusing on anxiety, depression, mood and quality of life as outcomes. The PRISMA statement was adopted. MEDLINE, PsycINFO, PUBMED and PsycArticles databases were used, and reference lists were examined for additional publications. In the selection of the articles were included studies considering women between 18 and 65 years who were diagnosed with breast cancer at any stage and under any treatment, and who underwent psychological group interventions. At the end of the systematic review, 45 studies met all inclusion criteria and were analysed in the meta-analysis. The overall effect size was medium, especially considering cognitive behavioural therapy and psycho-educational treatments. However, the studies are characterised by high methodological heterogeneity. Despite some limitations, this review and meta-analysis partially confirm the efficiency of cognitive-behavioural and psycho-educational therapies in the improvement of well-being in women with breast cancer.
Ultimate Image Singularities in Oblate Spheroidal Coordinates with Applications in Hydrodynamics
Authors: Chatjigeorgiou Ioannis K. , Loukogeorgaki Eva , Anastasiou Eirini , Mantadakis Nikos
Published: January 2020

This study exploits the Touvia Miloh oblate spheroid theorem with a special focus on hydrodynamical applications. The theorem provides explicit relations that express the oblate spheroidal harmonics, given in terms of the fundamental solutions of the Laplace equation. Here, the theorem is employed to transform the underlying Green’s function into the relevant coordinate system and, consequently, to formulate the diffraction potential. The case considered refers to the axisymmetric placement of the spheroid, namely, symmetrical axis perpendicular to the free surface. The mathematical formulations have been implemented numerically providing exceptionally accurate computations, which manifests the consistency and robustness of the relevant formulas.
Efficient Nonlinear Hydrodynamic Models for Wave Energy Converter Design—A Scoping Study
Authors: Davidson Josh , Costello Ronan
Published: January 2020

This review focuses on the most suitable form of hydrodynamic modeling for the next generation wave energy converter (WEC) design tools. To design and optimize a WEC, it is estimated that several million hours of operation must be simulated, perhaps one million hours of WEC simulation per year of the R&D program. This level of coverage is possible with linear potential flow (LPF) models, but the fidelity of the physics included is not adequate. Conversely, while Reynolds averaged Navier–Stokes (RANS) type computational fluid dynamics (CFD) solvers provide a high fidelity representation of the physics, the increased computational burden of these models renders the required amount of simulations infeasible. To scope the fast, high fidelity options, the present literature review aims to focus on what CFD theories exist intermediate to LPF and RANS as well as other modeling options that are computationally fast while retaining higher fidelity than LPF.
A Re-Evaluation of the Relationship between Morphology and Pathogenicity in Candida Species
Authors: Kadosh David , Mundodi Vasanthakrishna
Published: January 2020

Many pathogenic Candida species possess the ability to undergo a reversible morphological transition from yeast to filamentous cells. In Candida albicans, the most frequently isolated human fungal pathogen, multiple lines of evidence strongly suggest that this transition is associated with virulence and pathogenicity. While it has generally been assumed that non-albicans Candida species (NACS) are less pathogenic than C. albicans, in part, because they do not filament as well, definitive evidence is lacking. Interestingly, however, a recent study suggests that filamentation of NACS is associated with reduced, rather than increased, pathogenicity. These findings, in turn, challenge conventional views and suggest that there are fundamental evolutionary differences in the morphology–pathogenicity relationship in C. albicans vs. NACS. The findings also raise many new and intriguing questions and open new avenues for future research, which are discussed.
A Study on Fatigue Crack Propagation for Friction Stir Welded Plate of 7N01 Al-Zn-Mg Alloy by EBSD
Authors: Liu Wenyu , Wu Dongting , Duan Shuwei , Wang Tao , Zou Yong
Published: January 2020

EBSD (electron backscattered diffraction) was used to study the fatigue crack propagation mechanism in a friction stir welding joint of a 15 mm-thick 7N01 aluminum alloy plate. Crack tips with detailed features were clearly characterized by EBSD images. The plastic zone caused by crack was small in the stir zone. Due to the fine grain strengthening in the stir zone, there were several lattice distortion regions which were observed in the BC (band contrast) map but disappeared in the SEI (secondary electron image). In the stir zone, fatigue crack tends to awake and grow along grain boundaries, and propagate with little deformation of the grains. When the crack tries to grow across a boundary, the deformation of the plastic zone at the crack tip shows little correlation to the cyclic loading direction. However, the plastic zone in base metal, the rolled plate, is large and continuous, and no obvious lattice distortion region was found. According to Schmidt factor, the base metal near crack is fully deformed, lots of low angle boundaries parallel to the cyclic force can be observed. The base metal showed a better ability for fatigue crack propagation resistance.
A Fenton-Like Nanocatalyst Based on Easily Separated Magnetic Nanorings for Oxidation and Degradation of Dye Pollutant
Authors: Li Xiaonan , Li Jinghua , Shi Weilu , Bao Jianfeng , Yang Xianyuan
Published: January 2020

In this study, uniform Fe3O4 magnetic nanorings (Fe3O4-MNRs) were prepared through a simple hydrothermal method. The morphology, magnetic properties, and structure of the product were characterized by transmission electron microscope (TEM), scanning electron microscope (SEM), high resolution transmission electron microscopy (HRTEM), vibrating sample magnetometer (VSM), X-ray powder diffraction (XRD), and X-ray photoelectron spectroscopy (XPS), respectively. The Fe3O4-MNRs were used as Fenton-like catalysts in the presence of hydrogen peroxide (H2O2) and showed excellent Fenton-catalytic activity for degradation of organic dyes such as Methylene blue (MB), Rhodamine B (RhB), and Bromophenol blue (BPB). Furthermore, the obtained Fe3O4-MNRs could be recycled after used for several times and still remained in a relative high activity and could rapidly be separated from the reaction medium using a magnet without considerable loss. All results reveal that Fe3O4-MNRs have potential for the treatment of dyes pollutants.
Element-Doped Functional Carbon-Based Materials
Authors: Morales-Torres Sergio , Pérez-Cadenas Agustín F. , Carrasco-Marín Francisco
Published: January 2020

Carbon materials are one of the most fascinating materials because of their unique properties and potential use in several applications. They can be obtained from agricultural waste, organic polymers, or by using advanced synthesizing technologies. The carbon family is very wide, it includes classical activated carbons to more advanced types like carbon gels, graphene, and so on. The surface chemistry of these materials is one of the most interesting aspects to be studied. The incorporation of different types of chemical functionalities and/or heteroatoms such as O, N, B, S, or P on the carbon surface enables the modification of the acidic–basic character, hydrophilicity–hydrophobicity, and the electron properties of these materials, which in turn determines the final application. This book collects original research articles focused on the synthesis, properties, and applications of heteroatom-doped functional carbon materials.
Continuous Production of Pure Titanium with Ultrafine to Nanocrystalline Microstructure
Authors: Mertová Kateřina , Palán Jan , Duchek Michal , Studecký Tomáš , Džugan Jan , Poláková Ivana
Published: January 2020

This work deals with the application of the Conform SPD (Severe Plastic Deformation) continuous extrusion process for ultrafine to nanostructured pure titanium production. The process has been derived from the Equal Channel Angular Pressing (ECAP) technique but, unlike ECAP, it offers continuous production of high-strength wire. This study describes the Conform SPD process combined with subsequent cold working (rotary swaging technique), its potential for commercial application, and the properties of high-strength wires of pure titanium. High-strength wire of titanium Grade 4 is the product. Titanium Grade 4 reaches ultimate strengths up to 1320 MPa. This value is more than twice the ultimate strength of the unprocessed material. The typical grain size upon processing ranges from 200 to 500 nm. Process development supported by FEM analysis together with detailed microstructure characterization accompanied by mechanical properties investigation is presented.
Conjugates of Copper Alginate with Arginine-Glycine-Aspartic Acid (RGD) for Potential Use in Regenerative Medicine
Authors: Fraczyk Justyna , Wasko Joanna , Walczak Malgorzata , Kaminski Zbigniew J. , Puchowicz Dorota , Kaminska Irena , Bogun Maciej , Kolasa Marcin , Stodolak-Zych Ewa , Scislowska-Czarnecka Anna , Kolesinska Beata
Published: January 2020

Current restrictions on the use of antibiotics, associated with increases in bacterial resistance, require new solutions, including materials with antibacterial properties. In this study, copper alginate fibers obtained using the classic wet method were used to make nonwovens which were modified with arginine-glycine-aspartic acid (RGD) derivatives. Stable polysaccharide-peptide conjugates formed by coupling with 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium toluene-4-sulfonate (DMT/NMM/TosO−), and materials with physically embedded RGD derivatives, were obtained. The materials were found to be characterized by very high antibacterial activity against S. aureus and K. pneumoniae. Cytotoxicity studies confirmed that the materials are not cytotoxic. Copper alginate conjugates with RGD peptides have strong potential for use in regenerative medicine, due to their biocompatibility and innate antibacterial activity.
Performance Characterization of Semi-Flexible Composite Mixture
Authors: Zhang Weiguang , Shen Shihui , Goodwin Ryan Douglas , Wang Dalin , Zhong Jingtao
Published: January 2020

Semi-flexible composite mixture (SFCM) is developed based on a unique material design concept of pouring cement mortar into the voids formed by open graded asphalt mixture. It combines the flexibility of asphalt concrete and the stiffness of Portland cement concrete and has many advantages comparing to conventional roadway paving materials. The main objective of this paper was to evaluate the engineering properties of SFCM and assess the constructability of the SFCM. A slab SFCM sample was fabricated in the laboratory to simulate the filling of cement mortar in the field. Performance testing was carried out by indirect tensile (IDT) test because it was found to be able to correlate with the field performance of asphalt mixtures at low, intermediate, and high temperatures. They were used in this study to evaluate the thermal cracking, fatigue, rutting, as well as moisture resistance of SFCM. A control hot mix asphalt (HMA) mixture was used to compare with the results of SFCM. Based on the testing results, it was found that the designed SFCM showed good filling capability of cement mortar. SFCM had higher dynamic modulus than the control HMA. It had good resistance to rutting and moisture damage. Based on fracture work, SFCM showed better resistance to thermal cracking while lower resistance to fatigue cracking.
Continuous Ultrasonic Reactors: Design, Mechanism and Application
Authors: Dong Zhengya , Delacour Claire , Mc Carogher Keiran , Udepurkar Aniket Pradip , Kuhn Simon
Published: January 2020

Ultrasonic small scale flow reactors have found increasing popularity among researchers as they serve as a very useful platform for studying and controlling ultrasound mechanisms and effects. This has led to the use of these reactors for not only research purposes, but also various applications in biological, pharmaceutical and chemical processes mostly on laboratory and, in some cases, pilot scale. This review summarizes the state of the art of ultrasonic flow reactors and provides a guideline towards their design, characterization and application. Particular examples for ultrasound enhanced multiphase processes, spanning from immiscible fluid–fluid to fluid–solid systems, are provided. To conclude, challenges such as reactor efficiency and scalability are addressed.
Effects of Si/C Ratio on the Phase Composition of Si-C-N Powders Synthesized by Carbonitriding
Authors: Zhang Cong , Qu Ling , Yuan Wenjie
Published: January 2020

Si-C-N based materials possess interesting properties such as high hardness and oxidation resistance. The compacts of silicon and cornstarch with different Si/C ratios were subjected to carbonitriding at 1350–1550 °C. Reaction products were characterized by X-ray powder diffractometer (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and transmission electron microscope (TEM). The effects of Si/C ratio on the phase composition of Si-C-N powders were investigated. The results revealed that the Si/C ratio played a crucial role on the formation of crystalline silicon carbonitride (SiCN) and the phase composition of Si-C-N powders. It was demonstrated that liquid silicon is an important medium and reaction site for the introduction of nitrogen, so the Si content in reactants has affected the N content in the product. On the other hand, carbon participates in the carbonization of Si3N4 and the formation of SiC. The contents of C-N bond and SiCN in the products are carbon content-dependent. Combining the above two aspects, the maximum yield of SiCN can be achieved with the Si/C ratio of 1:1 to 1:1.5.