bluuebunny/crossref_metadata_2025 huggingface dataset:
Case Report: 21 Cases of Umbilical Hernia Repair Using a Laparoscopic Cephalic Approach Plus a Posterior Sheath and Extraperitoneal Approach
Authors: Zhang Kunjie , Qin Mingfang , Ding Guoqian
Published: September 2021

Purpose: In this study, a novel surgical technique was developed for umbilical hernias, in which a laparoscopic cephalic approach plus a posterior sheath and an extraperitoneal approach was employed. The aim of this study was to determine the results of this new technique.

Methods: From 2019 to 2020, 21 patients (81.8% men) with an umbilical hernia underwent a laparoscopic cephalic approach plus a posterior sheath and extraperitoneal approach, performed by two surgeons specializing in abdominal wall surgery, in two academic hospitals. Intraoperative and postoperative complications, operation time, blood loss, and hernia recurrence were assessed.

Results: Twenty-one cases of umbilical hernia were successfully completed. The size of the hernia ring was 1.5–3 cm2, with an average of 2.39 ± 0.47 cm2. The operation time was 120–240 min (average, 177.3 ± 42.15 min), and the blood loss volume was 30–40 ml (average, 33.73 ± 3.55 ml). The mean follow-up period was 6 months, and there were no short-term complications and no cases of recurrence.

Conclusion: A laparoscopic cephalic approach plus a posterior sheath and extraperitoneal approach is a safe alternative for the repair of an umbilical hernia. The intraoperative complication rate was low.
The Value of Magnetic Resonance Imaging Histograms in the Preoperative Differential Diagnosis of Endometrial Stromal Sarcoma and Degenerative Hysteromyoma
Authors: Zhang Xiao-Nan , Bai Man , Ma Ke-Ran , Zhang Yong , Song Cheng-Ru , Zhang Zan-Xia , Cheng Jing-Liang
Published: September 2021

Objective: The present study aimed to explore the application value of magnetic resonance imaging (MRI) histograms with multiple sequences in the preoperative differential diagnosis of endometrial stromal sarcoma (ESS) and degenerative hysteromyoma (DH).

Methods: The clinical and preoperative MRI data of 20 patients with pathologically confirmed ESS and 24 patients with pathologically confirmed DH were retrospectively analyzed, forming the two study groups. Mazda software was used to select the MRI layer with the largest tumor diameter in T2WI, the apparent diffusion coefficient (ADC), and enhanced T1WI (T1CE) images. The region of interest (ROI) was outlined for gray-scale histogram analysis. Nine parameters—the mean, variance, kurtosis, skewness, 1st percentile, 10th percentile, 50th percentile, 90th percentile, and 99th percentile—were obtained for intergroup analysis, and the receiver operating curves (ROCs) were plotted to analyze the differential diagnostic efficacy for each parameter.

Results: In the T2WI histogram, the differences between the two groups in seven of the parameters (mean, skewness, 1st percentile, 10th percentile, 50th percentile, 90th percentile, and 99th percentile) were statistically significant (P < 0.05). In the ADC histogram, the differences between the two groups in three of the parameters (skewness, 10th percentile, and 50th percentile) were statistically significant (P < 0.05). In the T1CE histogram, no significant differences were found between the two groups in any of the parameters (all P > 0.05). Of the nine parameters, the 50th percentile was found to have the best diagnostic efficacy. In the T2WI histogram, ROC curve analysis of the 50th percentile yielded the best area under the ROC curve (AUC; 0.742), sensitivity of 70%, and specificity of 83.3%. In the ADC histogram, ROC curve analysis of the 50th percentile yielded the best area under the ROC curve (AUC; 0.783), sensitivity of 81%, and specificity of 76.9%.

Conclusion: The parameters of the mean, 10th percentile and 50th percentile in the T2WI histogram have good diagnostic efficacy, providing new methods and ideas for clinical diagnosis.
Tubectomy of Pregnant and Non-pregnant Female Balinese Macaques (Macaca Fascicularis) With Post-operative Monitoring
Authors: Deleuze Stefan , Brotcorne Fany , Polet Roland , Soma Gede , Rigaux Goulven , Giraud Gwennan , Cloutier Fanny , Poncin Pascal , Wandia Nengah , Huynen Marie-Claude
Published: September 2021

Worldwide, primates, and humans increasingly share habitats and often enter in conflict when primates thrive in human-dominated environments, calling for special management measures. Reproductive control is increasingly used to manage population growth but very few monitoring data are available. Therefore, the efficiency and implications of such programs require a careful examination. In the context of a contraception program in wild female long-tailed macaques in Ubud, Bali, conducted over four successive campaigns between 2017 and 2019, including 140 females (i.e., 41.9% of the reproductive females of the population in 2019), modifications of an endoscopic tubectomy procedure, a permanent sterilization method, clinical evaluation of this method, and the post-operative monitoring results of the neutered females after release are described. This surgical approach was applicable for pregnant females: 28.6% of the treated females were pregnant at the time of the surgery. The procedure used a single lateral port to reach and cauterize both oviducts in non-pregnant as well as in early to mid-term pregnant females. Pregnant females nearer to term required a second lateral port to access both oviducts masked by the size of the gravid uterus. Moreover, bipolar thermocauterization was utilized successfully without resection to realize the tubectomy. The average duration of the laparoscopic surgery was 14 min for non-pregnant females and 22 min for pregnant females. Animals were released 3 h 22 min in average following their capture. This short holding time, recommended for free-ranging primates, was made possible by the minimal invasiveness of the sterilization approach. A laparoscopic post-operative evaluation conducted on two patients during the following campaign confirmed that the oviducts were definitely disrupted and no longer patent. Moreover, no new pregnancies in sterilized females were recorded during the 3-year observation period. The survival rate of the treated females 6 months after sterilization was high (96.3%) with no major post-operative complications clinically recorded. Among females that were pregnant during surgery, 81.1% were confirmed to experience term delivery. This study demonstrates the safety and efficiency of endoscopic tubectomy, even for pregnant females, as a mean of wild macaques' population control.
Fatal Calf Pneumonia Outbreaks in Italian Dairy Herds Involving Mycoplasma bovis and Other Agents of BRD Complex
Authors: Fanelli Angela , Cirilli Margie , Lucente Maria Stella , Zarea Aya Attia Koraney , Buonavoglia Domenico , Tempesta Maria , Greco Grazia
Published: September 2021

Mycoplasma bovis is increasingly recognized worldwide as an important cause of disease with major welfare and production impairments on cattle rearing. Although it was detected in veal calves and beef cattle, little is known on the infection impact and on its temporal morbidity pattern in Italian dairy herds. Thus, this study aimed to investigate the involvement of M. bovis on fatal calf pneumonia outbreaks that occurred during 2009–2019 in 64 Italian dairy farms. Furthermore, a deeper diagnostic workup of concurrent infection with other viral and bacterial respiratory pathogens was assessed. Out of the investigated fatal pneumonia cases, M. bovis was frequently detected (animal prevalence, 16.16%; 95%CI, 11.82–21.33; herd prevalence, 26.56; 95%CI, 16.29–39.08) either as the single agent of the disease in more than half of the positive samples (20/37) or in concurrent infections with Histophilus somni (9/37, 24.3%), Mannheimia haemolytica (6/37, 16.621%), Trueperella pyogenes (1/37, 2.70%), Pasteurella multocida (1/37, 2.70%), bovine respiratory syncytial virus (5/37, 13.51%), and bovine viral diarrhea virus (2/37, 5.55%). Based on time-series analysis, M. bovis was recorded in the area since 2009 with outbreaks displaying a clear morbidity seasonal pattern with peaks in April (43.21%) and in September (13.51%). This might be due to the stressing conditions during spring and late summer periods. Results of this study highlight that M. bovis infection warrants consideration, and control measures are needed given its involvement in lethal pneumonia outbreaks in dairy herds from an extended area.
Interaction Between PEDV and Its Hosts: A Closer Look at the ORF3 Accessory Protein
Authors: Jantraphakorn Yuparat , Viriyakitkosol Ratchanont , Jongkaewwattana Anan , Kaewborisuth Challika
Published: September 2021

Porcine epidemic diarrhea virus (PEDV) is a causative agent of a highly contagious enteric disease in swine of all ages, leading to severe economic losses for the swine industry in many countries. One of the most effective approaches in controlling PEDV infection is vaccination. The ORF3 accessory protein has been proposed as a crucial viral virulence factor in a natural host. However, due to the lack of an extensive comparative study of ORF3, exactly how the ORF3 takes part in virus replication and pathogenesis as well as its role in host-virus interaction is unclear. In this review, we aim to discuss the current knowledge of ORF3 concerning its dispensability for viral replication in vitro, ability to modulate host responses, contribution to virus pathogenicity, and research gaps among ORF3 functional studies. These will be beneficial for further studies to a better understanding of PEDV biology and PEDV vaccine development.
La metodología del aprendizaje basado en problemas en el logro de competencias en el curso de base de datos en la educación universitaria
Authors: García Díaz Bertila Liduvina , Oscanoa León Raúl Bernabé , Rivera Fritas Floresmilo
Published: September 2021

La investigación aplicada en el curso de Bases de Datos en la Escuela de Sistemas de una Universidad Estatal, usando la metodología del Aprendizaje Basado en Problemas, tuvo el objetivo de lograr competencias en el mencionado curso. Se buscó enfrentar problemas reales de la carrera. El tipo de estudio es Básico, cuantitativo, el diseño es cuasi- experimental, formado por 45 alumnos, divididos en un grupo de control y otro experimental. Como instrumento de medida se utilizó el test, que examinó solución de casos, y que fueron evaluados con rúbricas. Se evaluó las dimensiones de las competencias a lo largo del curso. Si analizamos los resultados de estas dimensiones: Diseño del Modelo Entidad Relación, Diseño de Bases de Datos y Diseño de Bases de Datos a partir de la Normalización los resultados son alentadores en el Post-test, ya que se evidencia un promedio de 27 en el grupo de control, que significa un promedio moderado en el límite inferior frente a un promedio de 44 en el grupo experimental, que significa un promedio sobresaliente. Se evidencia que hay un efecto significativo positivo del Aprendizaje Basado en Problemas en el logro de competencias en el curso de Base de Datos. (sig=0.000 < 0.05 rechaza la hipótesis nula)
Evaluación de la autoeficacia de emprendimiento y las capacidades de innovación en emprendedores del servicio turístico
Authors: Carranza Quimi Wendy Diana , Carranza Quimi Evelyn Janina , Manosalvas Vaca Luis Oswaldo
Published: September 2021

La investigación analiza el comportamiento emprendedor e innovador de los prestadores de servicios del sector turístico, utilizando como estudio de caso el Cantón Baños de Agua Santa, ubicado en la Provincia de Tungurahua. Se plantearon objetivos que van direccionados a diagnosticar, caracterizar y determinar la situación actual de los emprendedores y su influencia en el entorno que se desempeñan, de la misma manera, identificar si existe una relación entre la autoeficacia emprendedora y las capacidades de innovación. La metodología utilizada tiene un enfoque cuantitativo mediante el uso de instrumentos de medición validados y confiables para evaluar la autoeficacia emprendedora y las capacidades de innovación; el diseño de la investigación fue no experimental, con un horizonte transversal, de campo y documental con un análisis descriptivo y correlacional. Los resultados indicaron un perfil demográfico masculino predominante, con una edad joven adulta, y educación máxima alcanzada de bachillerato, la mayoría de estos emprendimientos pertenecen a servicios de alimentos y bebidas, seguido de alojamiento turístico, en donde no se identifica influencia recibida por familiares,  también se demostró que los emprendedores no tienen un nivel adecuado de autoeficacia emprendedora y capacidad innovadora lo que limita el desarrollo de la actividad de emprendimiento, sin embargo, las relaciones desarrolladas por las redes con los miembros de la comunidad y la intención de innovar es fuerte al igual que el afrontar cambios inesperados del entorno. Existe una correlación positiva y significativa entre la autoeficacia emprendedora y las capacidades de innovación.
Village Environment, Capital Endowment, and Farmers’ Participation in E-Commerce Sales Behavior: A Demand Observable Bivariate Probit Model Approach
Authors: Li Xiaojing , Sarkar Apurbo , Xia Xianli , Memon Waqar Hussain
Published: September 2021

With the recent developments in widespread internet usage and digital technology, an ultimate worldwide transformation in information and communications technology has occurred. Especially, how people engage in the virtual market for buying and selling goods has changed dramatically, which flourished the playground of electronic commerce (EC). Interestingly, it has become crucial to create an ample opportunity for farmers to utilize a more comprehensive market range for selling their products. However, farmers participating in e-commerce sales platforms may be interrupted by various internal and external factors. Therefore, the study’s primary goal is to evaluate the impacts of various external and internal factors on shaping farmers’ behavior in participating in e-commerce sales platforms. The study utilized a demand observable bivariate Probit model to analyze the village environment and capital endowment effects to craft the findings. The study utilized micro-survey data from 686 households in the leading kiwifruit-producing area as the empirical setup. The findings illustrated that the village environment is the main factor that restricts farmers’ e-commerce sales behavior, among which the infrastructure and policy environments have a significant contribution to farmers’ e-commerce sales intention and behavior. However, the effect of capital endowment on farmers’ e-commerce selling behavior has been found as significant. The village environment significantly affects both large- and small-scale farmers, and the capital endowment has a higher binding effect on small-scale farmers. Therefore, the paper suggests that improving the village environment for e-commerce development and laying the foundation for e-commerce development should be fostered. A differentiated incentive mechanism to improve the capital endowment of farmers should be constructed. A well-structured capital endowment triggering small farmers to capture the benefits of e-commerce sales should be imposed. The government should extend the support of the agricultural demonstration zone to facilitate practical training among the smallholder farmers. The formal and informal risk-sharing and financial institutions should prioritize building infrastructure to support farmers’ short- and long-term investments. Farmers should realize the importance of e-commerce for integrating the agricultural value chain.
Salix alba Clone Wilting Response to Heat Stress
Authors: Celma Santa , Vendina Viktorija , Lazdina Dagnija
Published: September 2021

The selection of most suitable propagation material, as well as the adjustment of existing seedling management practices, are gaining increasing importance to ensure the best outcomes under the long-term setting of climate change. One of the factors to consider is a predicted increase in the frequency and duration of high-temperature periods. Since heat often coincides with drought, these factors are typically assessed together, yet heat stress on its own has received less attention. In this study, we examined the effect of supra-optimal ambient temperature on nine Salix alba clone cuttings wilting under greenhouse conditions and sufficient moisture levels. Most plants are especially vulnerable to extreme conditions in early stages of development. Response to heat varied between clones but, overall, shoots that were taller than 350 mm were more sensitive to heat stress. The pruning of excess lateral shoots did not show significant improvement in heat tolerance. In total, 96% of the wilted cuttings proceeded to produce lateral shoots after the cessation of heat stress. It should be studied further if similar patterns are evident in field conditions and if earlier outplanting has a positive effect on cutting vitality.
High Serum Levels of Toxin A Correlate with Disease Severity in Patients with Clostridioides difficile Infection
Authors: Granata Guido , Mariotti Davide , Ascenzi Paolo , Petrosillo Nicola , di Masi Alessandra
Published: September 2021

Cloistridioides difficile (CD) represents a major public healthcare-associated infection causing significant morbidity and mortality. The pathogenic effects of CD are mainly caused by the release of two exotoxins into the intestine: toxin A (TcdA) and toxin B (TcdB). CD infection (CDI) can also cause toxemia, explaining the systemic complications of life-threatening cases. Currently, there is a lack of sensitive assays to detect exotoxins circulating in the blood. Here, we report a new semi-quantitative diagnostic method to measure CD toxins serum levels. The dot-blot assay was modified to separately detect TcdA and TcdB in human serum with a limit of detection at the pg/mL levels. TcdA and TcdB concentrations in the plasma of 35 CDI patients were measured at the time of CDI diagnosis and at the fourth and tenth day after CDI diagnosis and initiation of anti-CDI treatment. TcdA and TcdB levels were compared to those determined in nine healthy blood donors. Toxemia was detected in the plasma of 33 out of the 35 CDI cases. We also assessed the relationship between TcdA serum levels and CDI severity, reporting that at the time of CDI diagnosis the proportion of severe CDI cases with a TcdA serum level > 60 pg/µL was higher than in mild CDI cases (29.4% versus 66.6%, p = 0.04). In conclusion, data reported here demonstrate for the first time that toxemia is much more frequent than expected in CDI patients, and specifically that high serum levels of TcdA correlate with disease severity in patients with CDI.
Antimicrobial Peptides and Their Applications in Biomedical Sector
Authors: Sultana Afreen , Luo Hongrong , Ramakrishna Seeram
Published: September 2021

In a report by WHO (2014), it was stated that antimicrobial resistance is an arising challenge that needs to be resolved. This resistance is a critical issue in terms of disease or infection treatment and is usually caused due to mutation, gene transfer, long-term usage or inadequate use of antimicrobials, survival of microbes after consumption of antimicrobials, and the presence of antimicrobials in agricultural feeds. One of the solutions to this problem is antimicrobial peptides (AMPs), which are ubiquitously present in the environment. These peptides are of concern due to their special mode of action against a wide spectrum of infections and health-related problems. The biomedical field has the highest need of AMPs as it possesses prominent desirable activity against HIV-1, skin cancer, breast cancer, in Behcet’s disease treatment, as well as in reducing the release of inflammatory cells such as TNFα, IL-8, and IL-1β, enhancing the production of anti-inflammatory cytokines such as IL-10 and GM-CSF, and in wound healing properties. This review has highlighted all the major functions and applications of AMPs in the biomedical field and concludes the future potential of AMPs.
Comparison of Pharmaceutical Characteristics between Brand-Name Meropenem and Its Generics
Authors: Yang Ping , Fujimura Shigeru , Du Yawei , Zhang Bei , Yang Li , Kawamura Masato , Zhang Zhenhua , Zhai Suodi
Published: September 2021

This study aimed to provide comparative information of pharmaceutical properties, including particle morphology and distribution uniformity, solubility, presence of residual solvent and insoluble particles, and antimicrobial activities, between brand-name meropenem (Mepem®, BNM) and its six generic products (GPs A-F) marketed in China. Particles of GP-A and -C in dry powder had similar diameters of BNM, while other GPs were larger. Only BNM and GP-A were completely dissolved within 100 s in the lab condition. No insoluble particles >25 μm in diameter were detected in BNM and GP-E. Regarding stability of GPs solutions evaluated by concentration of open-ring metabolites at 6 h and 8 h, BNM showed the lowest open-ringed metabolite concentrates. Residual solvent of acetone detected in one GP showed the maximum value, while ethanol and ethyl acetate were detected both in product E and product F. The concordance rates (%) of minimum inhibitory concentration (MIC) of each generic compared to BNM were 89.5, 85, 87.5, 88, 88.5, and 86.5, respectively, although no significant difference was reached in MIC. Pharmaceutical characteristic differences between the BNM and GPs identified in this study could provide insights into understanding the deviations in the drug manufacturing processes of generic drugs.
The Influence of Oxidative Stress on Thyroid Diseases
Authors: Kochman Joanna , Jakubczyk Karolina , Bargiel Piotr , Janda-Milczarek Katarzyna
Published: September 2021

Thyroid diseases, including neoplasms, autoimmune diseases and thyroid dysfunctions, are becoming a serious social problem with rapidly increasing prevalence. The latter is increasingly linked to oxidative stress. There are many methods for determining the biomarkers of oxidative stress, making it possible to evaluate the oxidative profile in patients with thyroid diseases compared to the healthy population. This opens up a new perspective for investigating the role of elevated parameters of oxidative stress and damage in people with thyroid diseases, especially of neoplastic nature. An imbalance between oxidants and antioxidants is observed at different stages and in different types of thyroid diseases. The organ, which is part of the endocrine system, uses free radicals (reactive oxygen species, ROS) to produce hormones. Thyroid cells release enzymes that catalyse ROS generation; therefore, a key role is played by the internal defence system and non-enzymatic antioxidants that counteract excess ROS not utilised to produce thyroid hormones, acting as a buffer to neutralise free radicals and ensure whole-body homeostasis. An excess of free radicals causes structural cell damage, undermining genomic stability. Looking at the negative effects of ROS accumulation, oxidative stress appears to be implicated in both the initiation and progression of carcinogenesis. The aim of this review is to investigate the oxidation background of thyroid diseases and to summarise the links between redox imbalance and thyroid dysfunction and disease.
The Effects of a 4-Week Combined Aerobic and Resistance Training and Volleyball Training on Fitness Variables and Body Composition on STEAM Students
Authors: Pacholek Martin , Zemková Erika , Arnolds Keith , Šagát Peter
Published: September 2021

The study evaluates the effects of a 4-week program of combined resistance and aerobic training and volleyball training on physical fitness in young sedentary adults. Twenty-eight males (age 20.5 ± 1.5 years; body mass 87.2 ± 28.5 kg; height 173 ± 8.1 cm; BMI 28.9 ± 8.4 kg/m2) were divided into two groups. While experimental group 1 (COM) underwent a fitness program consisting of a combination of strength and aerobic exercises, experimental group 2 (VOL) performed intermittent exercises in volleyball (four times per week for 50 min). The results showed that both training groups significantly improved in all fitness tests except the beep test, while only the COM group achieved a significant change in the number of repetitions (p = 0.041). Between-group analyzes revealed a greater change achieved by the COM group in standing long jump lengths than VOL (12.3% vs. 4.3%, p = 0.011). There were no other between-group significant differences in 20m Multistage Fitness Test (8.1% and 4.4%,), sit-ups (20.9% and 21.0%), flexibility (24.5% and 23.3%) and shuttle run 5 × 10 m (11.95 and 9.52%) or in anthropometric parameters (BMI, Fat %, Muscle Mass %, Visceral Fat %). These findings indicate that combined resistance and aerobic training are more effective in improving the explosive power of lower limbs and aerobic endurance than playing an intermittent-type sport such as volleyball. This program can be effectively applied to university students with a predominantly sedentary lifestyle.
Exploiting the Combination of Displacement and Chemical Plating for a Tailored Electroless Deposition of Palladium Films on Copper
Authors: Fabbri Lorenzo , Giurlani Walter , Biffoli Fabio , Bellini Marco , Miller Hamish , Fontanesi Claudio , Vizza Francesco , Innocenti Massimo
Published: September 2021

Various formulations for electroless deposition, to obtain continuous nanometre-sized and micrometre-sized films of palladium on copper, were compared. We deposited ultrathin films using displacement plating formulations. We obtained continuous films with an equivalent thickness between 6 and 22 nm, measured by exploiting the K-ratio method with SEM-EDS of Pd layers. The Pd films obtained in this step of the work represent a cost-effective catalytic substrate. As a second step, we selected chemical plating as the procedure to obtain palladium films with a thickness in the micrometre range. An ammonia-based Pd chemical plating bath represent one of the most effective chemical plating formulations. To prevent copper substrates from being damaged by ammonia, displacement plating with palladium was also applied as a pre-treatment to make the use of these plating baths a viable way to obtain thicker palladium coatings. Palladium films showing good adherence, compact morphology, and a thickness over 1.5 μm were obtained, proving that the combination of two different electroless techniques was the key to develop a sustainable procedure for micrometre-sized palladium coatings, which could substitute electroplating of Pd in galvanic industry for decorative applications.
Aerial Robotic Solution for Detailed Inspection of Viaducts
Authors: Caballero Rafael , Parra Jesús , Trujillo Miguel Ángel , Pérez-Grau Francisco J. , Viguria Antidio , Ollero Aníbal
Published: September 2021

The inspection of public infrastructure, such as viaducts and bridges, is crucial for their proper maintenance given the heavy use of many of them. Current inspection techniques are very costly and manual, requiring highly qualified personnel and involving many risks. This article presents a novel solution for the detailed inspection of viaducts using aerial robotic platforms. The system provides a highly automated visual inspection platform that does not rely on GPS and could even fly underneath the infrastructure. Unlike commercially available solutions, our system automatically references the inspection to a global coordinate system usable throughout the lifespan of the infrastructure. In addition, the system includes another aerial platform with a robotic arm to make contact inspections of detected defects, thus providing information that cannot be obtained only with images. Both aerial robotic platforms feature flexibility in the choice of camera or contact measurement sensors as the situation requires. The system was validated by performing inspection flights on real viaducts.
Random Forests Highlight the Combined Effect of Environmental Heavy Metals Exposure and Genetic Damages for Cardiovascular Diseases
Authors: Monaco Alfonso , Lacalamita Antonio , Amoroso Nicola , D’Orta Armando , Del Buono Andrea , di Tuoro Francesco , Tangaro Sabina , Galeandro Aldo Innocente , Bellotti Roberto
Published: September 2021

Heavy metals are a dangerous source of pollution due to their toxicity, permanence in the environment and chemical nature. It is well known that long-term exposure to heavy metals is related to several chronic degenerative diseases (cardiovascular diseases, neoplasms, neurodegenerative syndromes, etc.). In this work, we propose a machine learning framework to evaluate the severity of cardiovascular diseases (CVD) from Human scalp hair analysis (HSHA) tests and genetic analysis and identify a small group of these clinical features mostly associated with the CVD risk. Using a private dataset provided by the DD Clinic foundation in Caserta, Italy, we cross-validated the classification performance of a Random Forests model with 90 subjects affected by CVD. The proposed model reached an AUC of 0.78 ± 0.01 on a three class classification problem. The robustness of the predictions was assessed by comparison with different cross-validation schemes and two state-of-the-art classifiers, such as Artificial Neural Network and General Linear Model. Thus, is the first work that studies, through a machine learning approach, the tight link between CVD severity, heavy metal concentrations and SNPs. Then, the selected features appear highly correlated with the CVD phenotype, and they could represent targets for future CVD therapies.
Development of a Coordinate Measuring Machine—Based Inspection Planning System for Industry 4.0
Authors: Stojadinovic Slavenko M. , Majstorovic Vidosav D. , Gąska Adam , Sładek Jerzy , Durakbasa Numan M.
Published: September 2021

Industry 4.0 represents a new paradigm which creates new requirements in the area of manufacturing and manufacturing metrology such as to reduce the cost of product, flexibility, mass customization, quality of product, high level of digitalization, optimization, etc., all of which contribute to smart manufacturing and smart metrology systems. This paper presents a developed inspection planning system based on CMM as support of the smart metrology within Industry 4.0 or manufacturing metrology 4.0 (MM4.0). The system is based on the application of three AI techniques such as engineering ontology (EO), GA and ants colony optimization (ACO). The developed system consists of: the ontological knowledge base; the mathematical model for generating strategy of initial MP; the model of analysis and optimization of workpiece setups and probe configuration; the path simulation model in MatLab, PTC Creo and STEP-NC Machine software, and the model of optimization MP by applying ACO. The advantage of the model is its suitability for monitoring of the measurement process and digitalization of the measurement process planning, simulation carried out and measurement verification based on CMM, reduction of the preparatory measurement time as early as in the inspection planning phase and minimizing human involvement or human errors through intelligent planning, which directly influences increased production efficiency, competitiveness, and productivity of enterprises. The measuring experiment was performed using a machined prismatic workpiece (PW).
Leaching Behavior of Cesium, Strontium, Cobalt, and Europium from Immobilized Cement Matrix
Authors: Goo Ja-Young , Kim Bong-Ju , Kang Myunggoo , Jeong Jongtae , Jo Ho Young , Kwon Jang-Soon
Published: September 2021

Numerous low-level and intermediate-level radioactive wastes are generated from the decommissioning processes of nuclear power plants; these wastes are immobilized to prevent the release of radionuclides under disposal conditions. In this study, we investigated the leaching behavior of Cs, Sr, Co, and Eu, which are common in immobilized decommissioning wastes. Ordinary Portland cement (OPC) was used as an immobilization agent. During the test, leaching of the nuclides occurred in the order of Cs, Sr, Co, and Eu and decreased over time. The results showed that 41.4% of the total Cs leached over 90 days, although the other elements leached in quantities less than 1.5%. CaCO3 was precipitated by the release of cementitious materials, indicating carbonation of the leachate. The leachability indexes in all cases exceeded the acceptable criteria (>6). The results of the present study suggest that OPC can be effectively used as a binding material to immobilize nuclides (Cs, Sr, Co, and Eu) contained in decommissioning wastes.
End-to-End Deep Reinforcement Learning for Image-Based UAV Autonomous Control
Authors: Zhao Jiang , Sun Jiaming , Cai Zhihao , Wang Longhong , Wang Yingxun
Published: September 2021

To achieve the perception-based autonomous control of UAVs, schemes with onboard sensing and computing are popular in state-of-the-art work, which often consist of several separated modules with respective complicated algorithms. Most methods depend on handcrafted designs and prior models with little capacity for adaptation and generalization. Inspired by the research on deep reinforcement learning, this paper proposes a new end-to-end autonomous control method to simplify the separate modules in the traditional control pipeline into a single neural network. An image-based reinforcement learning framework is established, depending on the design of the network architecture and the reward function. Training is performed with model-free algorithms developed according to the specific mission, and the control policy network can map the input image directly to the continuous actuator control command. A simulation environment for the scenario of UAV landing was built. In addition, the results under different typical cases, including both the small and large initial lateral or heading angle offsets, show that the proposed end-to-end method is feasible for perception-based autonomous control.
Diversity and Co-Occurrence Patterns of Fungal and Bacterial Communities from Alkaline Sediments and Water of Julong High-Altitude Hot Springs at Tianchi Volcano, Northeast China
Authors: Wang Xiao , Pecoraro Lorenzo
Published: September 2021

The Julong high-altitude volcanic hot springs in northeast China are of undeniable interest for microbiological studies due to their unique, extreme environmental conditions. The objective of this study was to provide a comprehensive analysis of the unexplored fungal and bacterial community composition, structure and networks in sediments and water from the Julong hot springs using a combination of culture-based methods and metabarcoding. A total of 65 fungal and 21 bacterial strains were isolated. Fungal genera Trichoderma and Cladosporium were dominant in sediments, while the most abundant fungi in hot spring water were Aspergillus and Alternaria. Bacterial communities in sediments and water were dominated by the genera Chryseobacterium and Pseudomonas, respectively. Metabarcoding analysis revealed significant differences in the microorganism communities from the two hot springs. Results suggested a strong influence of pH on the analyzed microbial diversity, at least when the environmental conditions became clearly alkaline. Our analyses indicated that mutualistic interactions may play an essential role in shaping stable microbial networks in the studied hot springs. The much more complicated bacterial than fungal networks described in our study may suggest that the more flexible trophic strategies of bacteria are beneficial for their survival and fitness under extreme conditions.
Pioglitazone Reverses Markers of Islet Beta-Cell De-Differentiation in db/db Mice While Modulating Expression of Genes Controlling Inflammation and Browning in White Adipose Tissue from Insulin-Resistant Mice and Humans
Authors: Collier J. Jason , Batdorf Heidi M. , Merrifield Kaelan L. , Martin Thomas M. , White Ursula , Ravussin Eric , Burk David H. , Cooley Chris R. , Karlstad Michael D. , Burke Susan J.
Published: September 2021

Obesity, insulin resistance, and type 2 diabetes contribute to increased morbidity and mortality in humans. The db/db mouse is an important mouse model that displays many key features of the human disease. Herein, we used the drug pioglitazone, a thiazolidinedione with insulin-sensitizing properties, to investigate blood glucose levels, indicators of islet β-cell health and maturity, and gene expression in adipose tissue. Oral administration of pioglitazone lowered blood glucose levels in db/db mice with a corresponding increase in respiratory quotient, which indicates improved whole-body carbohydrate utilization. In addition, white adipose tissue from db/db mice and from humans treated with pioglitazone showed increased expression of glycerol kinase. Both db/db mice and humans given pioglitazone displayed increased expression of UCP-1, a marker typically associated with brown adipose tissue. Moreover, pancreatic β-cells from db/db mice treated with pioglitazone had greater expression of insulin and Nkx6.1 as well as reduced abundance of the de-differentiation marker Aldh1a3. Collectively, these findings indicate that four weeks of pioglitazone therapy improved overall metabolic health in db/db mice. Our data are consistent with published reports of human subjects administered pioglitazone and with analysis of human adipose tissue taken from subjects treated with pioglitazone. In conclusion, the current study provides evidence that pioglitazone restores key markers of metabolic health and also showcases the utility of the db/db mouse to understand mechanisms associated with human metabolic disease and interventions that provide therapeutic benefit.
Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging
Authors: Zhang Jia , Gao Xinwei , Wang Luwei , Guo Yong , Zhu Yinru , Yang Zhigang , Yan Wei , Qu Junle
Published: September 2021

Stimulated emission depletion (STED) microscopy is a typical laser-scanning super-resolution imaging technology, the emergence of which has opened a new research window for studying the dynamic processes of live biological samples on a nanometer scale. According to the characteristics of STED, a high depletion power is required to obtain a high resolution. However, a high laser power can induce severe phototoxicity and photobleaching, which limits the applications for live cell imaging, especially in two-color STED super-resolution imaging. Therefore, we developed a low-power two-color STED super-resolution microscope with a single supercontinuum white-light laser. Using this system, we achieved low-power two-color super-resolution imaging based on digital enhancement technology. Lateral resolutions of 109 and 78 nm were obtained for mitochondria and microtubules in live cells, respectively, with 0.8 mW depletion power. These results highlight the great potential of the novel digitally enhanced two-color STED microscopy for long-term dynamic imaging of live cells.
Influence of Pressure on Product Composition and Hydrogen Consumption in Hydrotreating of Gas Oil and Rapeseed Oil Blends over a NiMo Catalyst
Authors: Blažek Josef , Toullis Daria , Straka Petr , Staš Martin , Šimáček Pavel
Published: September 2021

This study describes the co-hydrotreating of mixtures of rapeseed oil (0–20 wt%) with a petroleum feedstock consisting of 90 wt% of straight run gas oil and 10 wt% of light cycle oil. The hydrotreating was carried out in a laboratory flow reactor using a sulfided NiMo/Al2O3 catalyst at a temperature of 345 °C, the pressure of 4.0 and 8.0 MPa, a weight hourly space velocity of 1.0 h−1 and hydrogen to feedstock ratio of 230 m3∙m−3. All the liquid products met the EU diesel fuel specifications for the sulfur content (<10 mg∙kg−1). The content of aromatics in the products was very low due to the high hydrogenation activity of the catalyst and the total conversion of the rapeseed oil into saturated hydrocarbons. The addition of a depressant did not affect the cold filter plugging point of the products. The larger content of n-C17 than n-C18 alkanes suggested that the hydrodecarboxylation and hydrodecarbonylation reactions were preferred over the hydrodeoxygenation of the rapeseed oil. The hydrogen consumption increased with increasing pressure and the hydrogen consumption for the rapeseed oil conversion was higher when compared to the hydrotreating of the petroleum feedstock.
Remarkable Increase of Fluorescence Quantum Efficiency by Cyano Substitution on an ESIPT Molecule 2-(2-Hydroxyphenyl)benzothiazole: A Highly Photoluminescent Liquid Crystal Dopant
Authors: Sakurai Tsuneaki , Kobayashi Masaya , Yoshida Hiroyuki , Shimizu Masaki
Published: September 2021

Fluorescent molecules with excited-state intramolecular proton transfer (ESIPT) character allow the efficient solid-state luminescence with large Stokes shift that is important for various applications, such as organic electronics, photonics, and bio-imaging fields. However, the lower fluorescence quantum yields (ΦFL) in the solution or viscous media, due to their structural relaxations in the excited state to reach the S0/S1 conical intersection, shackle further applications of ESIPT-active luminophores. Here we report that the introduction of a cyano group (-CN) into the phenyl group of 2-(2-hydroxyphenyl)benzothiazole (HBT), a representative ESIPT compound, remarkably increase its fluorescence quantum yield (ΦFL) from 0.01 (without -CN) to 0.49 (with -CN) in CH2Cl2, without disturbing its high ΦFL (=0.52) in the solid state. The large increase of the solution-state ΦFL of the cyano-substituted HBT (CN-HBT) is remarkable, comparing with our previously reported ΦFL values of 0.05 (with 4-pentylphenyl), 0.07 (with 1-hexynyl), and 0.15 (with 4-pentylphenylethynyl). Of interest, the newly-synthesized compound, CN-HBT, is miscible in a conventional room-temperature nematic liquid crystal (LC), 4-pentyl-4′-cyano biphenyl (5CB), up to 1 wt% (~1 mol%), and exhibits a large ΦFL of 0.57 in the viscous LC medium. A similar ΦFL value of ΦFL = 0.53 was also recorded in another room-temperature LC, trans-4-(4-pentylcyclohexyl)benzonitrile (PCH5), with a doping ratio of 0.5 wt% (~0.5 mol%). These 5CB/CN-HBT and PCH5/CN-HBT mixtures serve as light-emitting room-temperature LCs, and show anisotropic fluorescence with the dichroic ratio of 3.1 upon polarized excitation, as well as electric field response of luminescence intensity changes.
Comparative Evaluation of Standard E TB-Feron ELISA and QuantiFERON-TB Gold Plus Assays in Patients with Tuberculosis and Healthcare Workers
Authors: Yoo In Young , Lee Jaewoong , Choi Ae Ran , Jun Yoon Hee , Lee Hwa Young , Kang Ji Young , Park Yeon-Joon
Published: September 2021

Recently, the American Thoracic Society/Infectious Diseases Society of America/Centers for Disease Control and Prevention advised against performing the interferon-γ-release assay (IGRA) test for individuals with a low risk of TB, and also recommended retesting low-risk individuals with an initial positive IGRA result. However, to evaluate both sensitivity and specificity of available tests, we compared the performance of the Standard E TB-Feron (TBF) and QuantiFERON-TB Gold Plus (QFT-Plus) assays in healthcare workers (HCWs) and tuberculosis (TB) patients. We also retrospectively investigated diabetes mellitus (DM) comorbidity among the enrolled TB patients. We prospectively collected samples from 177 HCWs and 48 TB patients. The TBF and QFT-Plus tests were performed and analyzed according to the manufacturers’ instructions. We also defined IGRA results between 0.2 and 0.7 IU/mL as ‘borderline’. The agreement rate between TBF and QFT-Plus was 92.0% (207/225) with a Cohen’s kappa value of 0.77 (95% CI, 0.68–0.87). While the majority (26/31, 83.9%) of borderline TBF results were in HCWs, the majority (14/19, 73.7%) of borderline QFT-Plus results were in TB patients. Discordant results were found in 18 samples, with TBF-positive/QFT-Plus-negative or indeterminate results in 11 HCWs and seven TB patients. After resampling from 10 HCWs (seven borderline-positive and three positive results, all <1.0), six reverted to negative. The prevalence of DM comorbidity was very high (35.4%). In summary, TBF showed substantial agreement with the QFT-Plus assay but had a higher positivity rate in both HCWs and TB patients. The negative conversion rate was high (60%) among HCWs whose initial (TB Ag-nil) result was <1.0.
Setting Individual Goals for Pupils with Profound Intellectual and Multiple Disabilities—Engaging in the Activity Area-Based Curriculum Making
Authors: Peltomäki Satu , Pirttimaa Raija , Pyhältö Kirsi , Kontu Elina K.
Published: September 2021

The activity area-based curriculum model (AACM) is used in the Finnish basic education to support pupils—most of whom have profound intellectual and multiple disabilities (PIMD)—who could benefit more from skills that advance independent life coping. Existing studies have concentrated mainly on the Individual Education Plan (IEP) goal-setting process for pupils with milder disabilities than PIMD and have consistently demonstrated significant barriers in the process. This study explores the collaborative IEP goal-setting process for pupils with the AACM. Sixty-five Finnish special education teachers using the AACM participated in the study. Semi-structured interviews were analysed with thematic network analysis. The groups involved in the IEP goal-setting process were divided into main and side mediators. The main mediators were described as significant collaborators with important knowledge of the pupil, while side mediators rarely participated in the process. The special education teachers appeared to be leading the collaboration and were positioned in the middle of the main and side mediators. Further research should focus on the implementation of parents’ collaborative positions. Furthermore, practices and methods should be developed to reinforce the positions of group home staff, special education teacher colleagues and pupils with the AACM or PIMD themselves.
Physics-Informed Neural Network for High Frequency Noise Performance in Quasi-Ballistic MOSFETs
Authors: Lee Jonghwan
Published: September 2021

A physics-informed neural network (PINN) model is presented to predict the nonlinear characteristics of high frequency (HF) noise performance in quasi-ballistic MOSFETs. The PINN model is formulated by combining the radial basis function-artificial neural networks (RBF-ANNs) with an improved noise equivalent circuit model, including all the noise sources. The RBF-ANNs are utilized to model the thermal channel noise, induced gate noise, correlation noise, as well as the shot noise, due to the gate and source-drain tunneling current through the potential barriers. By training a spatial distribution of the thermal channel noise and a Fano factor of the shot noise, underlying physical theories are naturally embedded into the PINN model as prior information. The PINN model shows good capability of predicting the noise performance at high frequencies.
Generative Adversarial Networks for Anonymized Healthcare of Lung Cancer Patients
Authors: Gonzalez-Abril Luis , Angulo Cecilio , Ortega Juan-Antonio , Lopez-Guerra José-Luis
Published: September 2021

The digital twin in health care is the dynamic digital representation of the patient’s anatomy and physiology through computational models which are continuously updated from clinical data. Furthermore, used in combination with machine learning technologies, it should help doctors in therapeutic path and in minimally invasive intervention procedures. Confidentiality of medical records is a very delicate issue, therefore some anonymization process is mandatory in order to maintain patients privacy. Moreover, data availability is very limited in some health domains like lung cancer treatment. Hence, generation of synthetic data conformed to real data would solve this issue. In this paper, the use of generative adversarial networks (GAN) for the generation of synthetic data of lung cancer patients is introduced as a tool to solve this problem in the form of anonymized synthetic patients. Generated synthetic patients are validated using both statistical methods, as well as by oncologists using the indirect mortality rate obtained for patients in different stages.