bluuebunny/crossref_metadata_2025 huggingface dataset:
Control Chart T2Qv for Statistical Control of Multivariate Processes with Qualitative Variables
Authors: Rojas-Preciado Wilson , Rojas-Campuzano Mauricio , Galindo-Villardón Purificación , Ruiz-Barzola Omar
Published: June 2023

The scientific literature is abundant regarding control charts in multivariate environments for numerical and mixed data; however, there are few publications for qualitative data. Qualitative variables provide valuable information on processes in various industrial, productive, technological, and health contexts. Social processes are no exception. There are multiple nominal and ordinal categorical variables used in economics, psychology, law, sociology, and education, whose analysis adds value to decision-making; therefore, their representation in control charts would be useful. When there are many variables, there is a risk of redundant or excessive information, so the application of multivariate methods for dimension reduction to retain a few latent variables, i.e., a recombination of the original and synthesizing of most of the information, is viable. In this context, the T2Qv control chart is presented as a multivariate statistical process control technique that performs an analysis of qualitative data through Multiple Correspondence Analysis (MCA), and the Hotelling T2 chart. The interpretation of out-of-control points is carried out by comparing MCA charts and analyzing the χ2 distance between the categories of the concatenated table and those that represent out-of-control points. Sensitivity analysis determined that the T2Qv control chart performs well when working with high dimensions. To test the methodology, an analysis was performed with simulated data and with a real case applied to the graduate follow-up process in the context of higher education. To facilitate the dissemination and application of the proposal, a reproducible computational package was developed in R, called T2Qv, and is available on the Comprehensive R Archive Network (CRAN).
Exponential Stability of a Class of Neutral Inertial Neural Networks with Multi-Proportional Delays and Leakage Delays
Authors: Wang Chao , Song Yinfang , Zhang Fengjiao , Zhao Yuxiao
Published: June 2023

This paper investigates the exponential stability of a class of neutral inertial neural networks with multi-proportional delays and leakage delays. By utilizing the Lyapunov stability theory, the approach of parametric variation, and the differential inequality technique, some criteria are acquired that can guarantee that all solutions of the addressed system converge exponentially to the equilibrium point. In particular, the neutral term, multi-proportional delays, and leakage delays are incorporated simultaneously, resulting in a more general model, and the findings are novel and refine the previous works. Finally, one example is provided to indicate that the dynamic behavior is consistent with the theoretical analysis.
Use of High-Resolution Ultrasound in Characterizing the Surface Topography of a Breast Implant
Authors: Kim Yang-Hee , Park Dong-Wook , Song Keun-Yeong , Lim Hyung-Guhn , Jeong Jeong-Pil , Kim Jae-Hong
Published: June 2023

Background and Objectives: With the emergence of breast implant-associated anaplastic large cell lymphoma (BIA-ALCL), it has become necessary to identify the implant shell type patients have received. Therefore, an immediate, reliable method for identifying a breast implant shell type is essential. Evidence-based research and applying a real-world technique that identifies the surface topographic information of the inserted breast implants, without surgery, has become of paramount importance for breast implant physicians. Methods and Materials: A review of the medical records of 1901 patients who received 3802 breast implants and subsequently received an ultrasound-assisted examination was performed. All patients received not only a breast cancer examination but also a high-resolution ultrasonography (HRUS) assisted examination of the device at a single center between 31 August 2017 and 31 December 2022. Results: Most patients had breast implants within 10 years (77.7%) of the examination. Of the 3802 implants screened, 2034 (53.5%) were identified with macro-textured shell topography in ultrasonography. A macrotextured shell type implant was used in 53.5% of cases and a smooth type in 42.7% of cases. Seventy-three (1.9%) breast implant shell types could not be identified due to ruptures. However, 250 breast implant shell types could be identified despite rupture cases (6.5%). Conclusions: HRUS was found to be a useful and reliable image modality for identifying various surface shell types of breast implants. The shell type information would be helpful to patients who lack information about their breast implants and are concerned about BIA-ALCL.
The Smallpox Vaccine in Latin America: A New Approach (1801–1804)
Authors: Pérez Pérez Antonio , Vallejo José Ramón
Published: June 2023

The Royal Philanthropic Vaccine Expedition is considered in the history of medicine as the first international health expedition aimed at the global elimination of a contagious disease: smallpox. However, the initiatives carried out in this way before the arrival of the Balmis Expedition, by surgeons from the Spanish Navy, are less well known. Thus, the main objective of this research work is to offer an overview of the different anti-variolic vaccination initiatives prior to the campaign financed by the Spanish crown from these health facilities. Using the heuristic and hermeneutic method, our article is based on primary sources contrasted with specialised literature. The results obtained are presented in a narrative style from each of the surgeons identified as decisive in the implementation of the vaccine, thus providing a divergent and unpublished historiographic approach. As the facts described show, before the arrival of Balmis the vaccine substance was introduced in those countries thanks to the initiative of various surgeons: in Puerto Rico by Francisco Oller; in Cartagena and Santa Marta in Colombia by Ángel Hidalgo; in Venezuela by Alonso Ruiz; in Cuba by Tomás Romay and Bernardo de Cózar; in the Viceroyalty of New Granada (Colombia) by Lorenzo Vergés; in Guatemala by Miguel José Monzón and José María Ledesma; in the Viceroyalty of New Spain by Alejandro García Arboleya and Antonio Serrano; in Peru by Pedro Belomo; in Río de la Plata by Cristóbal Martín de Montúfar; in the Chilean region of Coquimbo by José María Gómez; and in the Philippines by Cristóbal Regidor. Finally, it should be noted that these surgeons and the approach presented are part of a historiography based on the personal actions of professionals trained, for the most part, at the Medical–Surgical School of Cadiz.
Evaluating the Residual Life of Long-Term Equipment Made of Structural and Heat-Resistant Steel by Using the Structural–Mechanical Criterion
Authors: Ababkov Nikolay , Smirnov Alexandr , Danilov Vladimir
Published: June 2023

The use of acoustic and magnetic methods of non-destructive testing to detect zones of stable localization of deformation in order to assess and predict the performance of long-term equipment is of scientific and practical interest at present. A structural–mechanical criterion was developed that reflects the relationships between the structural and substructural states, internal stress fields and stable localization of deformations with the characteristics of non-destructive tests in the metal of long-term equipment made of structural 0.2 C steel and heat-resistant 0.12C-1Cr-1Mo-1V steel. The values of the structural–mechanical criteria Ks.-m for structural 0.2 C steel and for heat-resistant 0.12C-1Cr-1Mo-1V steel, corresponding to the moment of stable localization of deformation, are established. At the same time, it is recommended to replace the checked equipment nodes due to the exhaustion of the resource. The proposed and justified approach to assessing and predicting the performance and residual life of long-term power equipment, based on the identified relationships between the structural and substructural states, internal stress fields and stable localization of deformations with the characteristics of non-destructive tests and the calculation of the structural–mechanical criterion, was applied at a number of power plants in the Kemerovo region—Kuzbass. A methodology was developed for evaluating the residual life, based on the identification and use of relationships between structural and substructural states, internal stress fields and stable localization of deformations with the characteristics of non-destructive tests and the calculation of a structural–mechanical criterion.
Emerging SiC Applications beyond Power Electronic Devices
Authors: La Via Francesco , Alquier Daniel , Giannazzo Filippo , Kimoto Tsunenobu , Neudeck Philip , Ou Haiyan , Roncaglia Alberto , Saddow Stephen E. , Tudisco Salvatore
Published: June 2023

In recent years, several new applications of SiC (both 4H and 3C polytypes) have been proposed in different papers. In this review, several of these emerging applications have been reported to show the development status, the main problems to be solved and the outlooks for these new devices. The use of SiC for high temperature applications in space, high temperature CMOS, high radiation hard detectors, new optical devices, high frequency MEMS, new devices with integrated 2D materials and biosensors have been extensively reviewed in this paper. The development of these new applications, at least for the 4H-SiC ones, has been favored by the strong improvement in SiC technology and in the material quality and price, due to the increasing market for power devices. However, at the same time, these new applications need the development of new processes and the improvement of material properties (high temperature packages, channel mobility and threshold voltage instability improvement, thick epitaxial layers, low defects, long carrier lifetime, low epitaxial doping). Instead, in the case of 3C-SiC applications, several new projects have developed material processes to obtain more performing MEMS, photonics and biomedical devices. Despite the good performance of these devices and the potential market, the further development of the material and of the specific processes and the lack of several SiC foundries for these applications are limiting further development in these fields.
Multi-Omics Analysis Demonstrates the Critical Role of Non-Ethanolic Components of Alcoholic Beverages in the Host Microbiome and Metabolome: A Human- and Animal-Based Study
Authors: Sarkar Priyanka , Kandimalla Raghuram , Bhattacharya Anupam , Wahengbam Romi , Dehingia Madhusmita , Kalita Mohan Chandra , Talukdar Narayan Chandra , Talukdar Rupjyoti , Khan Mojibur R.
Published: June 2023

It is known that alcoholic beverages alter the human gut microbiome. This study focused on the potential impact of non-ethanolic ingredients in whisky on the gut bacteriome. A pilot study was carried out on 15 whisky drinkers, 5 rice beer drinkers, and 9 non-drinkers to determine the effect of alcoholic beverages on the host microbiome and metabolome. Additionally, a mouse model was used to assess the differential impact of three whisky brands (each with an equal ethanol concentration). The results indicate that the non-ethanolic components have an impact on the gut microbiome, as well as on the metabolites in blood and feces. The amount of Prevotella copri, a typical core Indian gut bacterium, decreased in both the human and mouse groups of whisky type 1, but an increase in abundance of Helicobacteriaceae (p = 0.01) was noticed in both groups. Additionally, the alcohol-treated cohorts had lower levels of short-chain fatty acids (SCFAs), specifically butyric acid, and higher amounts of lipids and stress marker IL1-ß than the untreated groups (p = 0.04–0.01). Furthermore, two compounds, ethanal/acetaldehyde (found in all the whisky samples) and arabitol (unique to whisky type 1), were tested in the mice. Similar to the human subjects, the whisky type 1 treated mouse cohort and the arabitol-treated group showed decreased levels of Prevotella copri (p = 0.01) in their gut. The results showed that non-ethanolic compounds have a significant impact on host gut bacterial diversity and metabolite composition, which has a further vital impact on host health. Our work further emphasizes the need to study the impact of non-ethanolic ingredients of alcoholic beverages on host health.
A Microbial Co-Culturing System for Producing Cellulose-Hyaluronic Acid Composites
Authors: Brugnoli Marcello , Mazzini Ilaria , La China Salvatore , De Vero Luciana , Gullo Maria
Published: June 2023

In this study, a co-culture system combining bacterial cellulose (BC) producers and hyaluronic acid (HA) producers was developed for four different combinations. AAB of the genus Komagataeibacter sp. and LAB of the Lactocaseibacillus genus were used to produce BC and HA, respectively. Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate changes in BC-HA composites chemical and morphological structure. Water absorption, uptake, and antibacterial properties were also tested. Outcomes highlighted a higher bacterial cellulose yield and the incorporation of hyaluronic acid into the composite. The presence of hyaluronic acid increased fiber dimension—nearly doubled for some combinations—which led to a decreased crystallinity of the composites. Different results were observed based on the BC producer and HA producer combination. However, water holding capacity (WHC) in all the samples improved with the presence of HA, while water uptake worsened. A thymol-enriched BC-HA composite showed high antibacterial activity against Escherichia coli DSM 30083T and Staphylococcus aureus DSM 20231T. Results could contribute to opening new applications in the cosmetics or pharmaceutical fields.
Antioxidant, Anti-Inflammatory, and Anti-Cancer Properties of Amygdalin Extracted from Three Cassava Varieties Cultivated in Benin
Authors: Lehmane Halfane , Kohonou Arnaud N. , Tchogou Atchadé Pascal , Ba Radiate , Dah-Nouvlessounon Durand , Didagbé Oscar , Sina Haziz , Senou Maximin , Adjanohoun Adolphe , Baba-Moussa Lamine
Published: June 2023

Given that cancer is a disease that is rampant in the world and especially in Africa, where the population has enormous difficulty treating it, plants are a safer and less expensive alternative. Cassava is a plant species valued in Benin because of its numerous medicinal and nutritional virtues. This study evaluated the biological activities of amygdalin from the organs of three cassava varieties most commonly produced in Benin (BEN, RB, and MJ). HPLC analysis was used to quantify amygdalin in cassava organs and derivatives. Phytochemical screening was performed to determine secondary metabolite groups. DPPH and FRAP methods were used to assess antioxidant activity. Cytotoxicity of the extracts was tested on Artemia salina larvae. The anti-inflammatory activity was evaluated in vivo in an albino mouse paw edema model induced by 5% formalin. The anticancer activity was evaluated in vivo on Wistar rats rendered cancerous by 1,2-dimethylhydrazine (DMH) using 5-fluorouracil as a reference molecule. The results showed that the organs of all three-cassava varieties contained glycosides, flavonoids, saponosides, steroids, tannins, coumarins, and cyanogenic derivatives. Young stems and fresh cassava leaves had the highest amygdalin concentrations, with 11,142.99 µg 10 g−1 and 9251.14 µg 10 g−1, respectively. The Agbeli derivative was more concentrated in amygdalin, with a content of 401.56 µg 10 g−1 than the other derivatives. The antioxidant activity results showed that the amygdalin extracts were DPPH radical scavengers with IC50 values ranging from 0.18 mg mL−1 to 2.35 mg mL−1. The cytotoxicity test showed no toxicity of the extracts toward shrimp larvae. Administration of amygdalin extracts from the leaves of BEN and MJ varieties prevents inflammatory edema. The percentages of edema inhibition varied between 21.77% and 27.89%. These values are similar (p > 0.05) to those of acetylsalicylic acid (25.20%). Amygdalin extract of the BEN variety significantly (p < 0.0001) reduces edema. Both BEN extracts inhibited cancer induction with DMH. In preventive and curative treatments, rats fed with amygdalin extracts showed low anti-cancer activity under the effect of DMH and a significant difference in biochemical results. Thus, the organs of all three cassava varieties studied have secondary metabolites and good antioxidant activity. The leaves contain high levels of amygdalin and can be used as anti-inflammatory and anticancer agents.
Antioxidant Activities of Essential Oils and Their Major Components in Scavenging Free Radicals, Inhibiting Lipid Oxidation and Reducing Cellular Oxidative Stress
Authors: Chen Xiaohua , Shang Shufeng , Yan Fei , Jiang Hai , Zhao Guanjie , Tian Shan , Chen Rui , Chen Dejing , Dang Yafeng
Published: June 2023

Antioxidant activities of five essential oils (cinnamon, thyme, clove, lavender and peppermint oils) and their major components (eugenol, thymol, linalool, and menthol) were evaluated on scavenging DPPH (2,2-diphenyl-1 picrylhydrazyl) free radicals, inhibiting polyunsaturated fatty acid oxidation in fish oil emulsion (FOE), and reducing oxidative stress in human red blood cells (RBC). The essential oils from cinnamon, thyme, clove and their main components, eugenol and thymol, exhibited the highest antioxidant activity in the FOE and RBC systems. It was found that the antioxidant activity of essential oils was positively correlated to the content of eugenol and thymol, while lavender and peppermint oils and their main components, linalool and menthol, had very low antioxidant activity. Compared with scavenging DPPH free radical activity, the antioxidant activity in FOE and RBC systems could better reflect the actual antioxidant potential of essential oil in preventing lipid oxidation and reducing oxidative stress in biological system.
Structural and Spectroscopic Study of New Copper(II) and Zinc(II) Complexes of Coumarin Oxyacetate Ligands and Determination of Their Antimicrobial Activity
Authors: Mujahid Muhammad , Trendafilova Natasha , Rosair Georgina , Kavanagh Kevin , Walsh Maureen , Creaven Bernadette S. , Georgieva Ivelina
Published: June 2023

Tackling antimicrobial resistance is of increasing concern in a post-pandemic world where overuse of antibiotics has increased the threat of another pandemic caused by antimicrobial-resistant pathogens. Derivatives of coumarins, a naturally occurring bioactive compound, and its metal complexes have proven therapeutic potential as antimicrobial agents and in this study a series of copper(II) and zinc(II) complexes of coumarin oxyacetate ligands were synthesised and characterised by spectroscopic techniques (IR, 1H, 13C NMR, UV-Vis) and by X-ray crystallography for two of the zinc complexes. The experimental spectroscopic data were then interpreted on the basis of molecular structure modelling and subsequent spectra simulation using the density functional theory method to identify the coordination mode in solution for the metal ions in the complexes. Interestingly, the solid-state coordination environment of the zinc complexes is in good agreement with the simulated solution state, which has not been the case in our previous studies of these ligands when coordinated to silver(I). Previous studies had indicated excellent antimicrobial activity for Ag(I) analogues of these ligands and related copper and zinc complexes of coumarin-derived ligands, but in this study none of the complexes displayed antimicrobial activity against the clinically relevant methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Candida albicans.
Spectroscopic Constants and Anharmonic Vibrational Frequencies of C(O)OC, c-C2O2 and Their Silicon-Containing Analogues
Authors: Harwick Olivia A. , Fortenberry Ryan C.
Published: June 2023

Comets are likely to contain various carbon oxide molecules potentially including C(O)OC and c-C2O2 on their surfaces and comae, as well as their silicon-substituted analogues possibly playing a role in the formation of interstellar dust grains. In this work, high-level quantum chemical data are provided to support such potential future astrophysical detection through the generation of predicted rovibrational data. Laboratory-based chemistry would also benefit from such aforementioned computational benchmarking considering these molecules’ historic computational and experimental elusiveness. Coupled-cluster singles, doubles, and perturbative triples, the F12b formalism, and the cc-pCVTZ-F12 basis set garner the rapid, yet highly trusted F12-TcCR level of theory leveraged presently. This current work points to all four molecules’ strong IR activity, coupled with large intensities, thus suggesting the potential for JWST detection. Although Si(O)OSi possesses a permanent dipole moment significantly larger than those of the other molecules of present interest, the significant abundance of the potential precursor carbon monoxide suggests that the dicarbon dioxide molecules may yet be observable in the microwave region of the electromagnetic spectrum. Thus, this present work details the likely existence and detectability of these four cyclic molecules, providing updated implications compared to previous work performed both experimentally and computationally.
1,3-Butadiynamides the Ethynylogous Ynamides: Synthesis, Properties and Applications in Heterocyclic Chemistry
Authors: Lenko Illia , Alayrac Carole , Bożek Igor , Witulski Bernhard
Published: June 2023

1,3-butadiynamides—the ethynylogous variants of ynamides—receive considerable attention as precursors of complex molecular scaffolds for organic and heterocyclic chemistry. The synthetic potential of these C4-building blocks reveals itself in sophisticated transition-metal catalyzed annulation reactions and in metal-free or silver-mediated HDDA (Hexa-dehydro-Diels–Alder) cycloadditions. 1,3-Butadiynamides also gain significance as optoelectronic materials and in less explored views on their unique helical twisted frontier molecular orbitals (Hel-FMOs). The present account summarizes different methodologies for the synthesis of 1,3-butadiynamides followed by the description of their molecular structure and electronic properties. Finally, the surprisingly rich chemistry of 1,3-butadiynamides as versatile C4-building blocks in heterocyclic chemistry is reviewed by compiling their exciting reactivity, specificity and opportunities for organic synthesis. Besides chemical transformations and use in synthesis, a focus is set on the mechanistic understanding of the chemistry of 1,3-butadiynamides—suggesting that 1,3-butadiynamides are not just simple alkynes. These ethynylogous variants of ynamides have their own molecular character and chemical reactivity and reflect a new class of remarkably useful compounds.
Novel Mn4+-Activated K2Nb1−xMoxF7 (0 ≤ x ≤ 0.15) Solid Solution Red Phosphors with Superior Moisture Resistance and Good Thermal Stability
Authors: Gao Yuhan , Feng Lei , Wang Linglin , Zheng Jun , Ren Feiyao , Liu Siyu , Ning Zhanglei , Zhou Ting , Wu Xiaochun , Lai Xin , Gao Daojiang
Published: June 2023

Nowadays, Mn4+-activated fluoride red phosphors with excellent luminescence properties have triggered tremendous attentions for enhancing the performance of white light-emitting diodes (WLEDs). Nonetheless, the poor moisture resistance of these phosphors impedes their commercialization. Herein, we proposed the dual strategies of “solid solution design” and “charge compensation” to design K2Nb1−xMoxF7 novel fluoride solid solution system, and synthesized the Mn4+-activated K2Nb1−xMoxF7 (0 ≤ x ≤ 0.15, x represents the mol % of Mo6+ in the initial solution) red phosphors via co-precipitation method. The doping of Mo6+ not only significantly improve the moisture resistance of the K2NbF7: Mn4+ phosphor without any passivation and surface coating, but also effectively enhance the luminescence properties and thermal stability. In particular, the obtained K2Nb1−xMoxF7: Mn4+ (x = 0.05) phosphor possesses the quantum yield of 47.22% and retains 69.95% of its initial emission intensity at 353 K. Notably, the normalized intensity of the red emission peak (627 nm) for the K2Nb1−xMoxF7: Mn4+ (x = 0.05) phosphor is 86.37% of its initial intensity after immersion for 1440 min, prominently higher than that of the K2NbF7: Mn4+ phosphor. Moreover, a high-performance WLED with high CRI of 88 and low CCT of 3979 K is fabricated by combining blue chip (InGaN), yellow phosphor (Y3Al5O12: Ce3+) and the K2Nb1−xMoxF7: Mn4+ (x = 0.05) red phosphor. Our findings convincingly demonstrate that the K2Nb1−xMoxF7: Mn4+ phosphors have a good practical application in WLEDs.
Titanium-Mediated Reduction of Carboxamides to Amines with Borane–Ammonia
Authors: Ramachandran P. Veeraraghavan , Alawaed Abdulkhaliq A. , Singh Aman
Published: June 2023

In this study, the successful titanium tetrachloride-catalyzed reduction of aldehydes, ketones, carboxylic acids, and nitriles with borane–ammonia was extended to the reduction (deoxygenation) of a variety of aromatic and aliphatic pri-, sec- and tert-carboxamides, by changing the stoichiometry of the catalyst and reductant. The corresponding amines were isolated in good to excellent yields, following a simple acid–base workup.
Controllable Synthesis and Charge Density Wave Phase Transitions of Two-Dimensional 1T-TaS2 Crystals
Authors: Pan Xiaoguang , Yang Tianwen , Bai Hangxin , Peng Jiangbo , Li Lujie , Jing Fangli , Qiu Hailong , Liu Hongjun , Hu Zhanggui
Published: June 2023

1T-TaS2 has attracted much attention recently due to its abundant charge density wave phases. In this work, high-quality two-dimensional 1T-TaS2 crystals were successfully synthesized by a chemical vapor deposition method with controllable layer numbers, confirmed by the structural characterization. Based on the as-grown samples, their thickness-dependency nearly commensurate charge density wave/commensurate charge density wave phase transitions was revealed by the combination of the temperature-dependent resistance measurements and Raman spectra. The phase transition temperature increased with increasing thickness, but no apparent phase transition was found on the 2~3 nm thick crystals from temperature-dependent Raman spectra. The transition hysteresis loops due to temperature-dependent resistance changes of 1T-TaS2 can be used for memory devices and oscillators, making 1T-TaS2 a promising material for various electronic applications.
Dextran-Coated Iron Oxide Nanoparticles Loaded with 5-Fluorouracil for Drug-Delivery Applications
Authors: Predoi Daniela , Balas Mihaela , Badea Madalina Andreea , Ciobanu Steluta Carmen , Buton Nicolas , Dinischiotu Anca
Published: June 2023

This study aims to design and test different formulations composed of dextran-coated iron oxide nanoparticles (IONPs) loaded with 5-Fluorouracil (5-FU) with varying nanoparticle:drug ratios on colorectal cancer cells. The stable suspension of IONPs s was synthesized by the adapted co-precipitation method. The stable suspension of IONPs was mixed with a solution of dextran and 5-FU solubilized in a saline solution. The final suspensions with optimized ratios of IONP:5-FU in the final suspension were 0.5:1, 1:1, and 1.5:1. The information on the morphology and size distribution of the IONPs suspension and IONP loads with 5-FU was obtained using scanning electron microscopy (SEM). The presence of 5-FU and dextran on the surface of the IONPs was highlighted by energy-dispersive X-ray spectroscopy (EDS) studies. The determination of the surface charge of the nanoparticles in the final suspensions of IONP:5-FU was achieved by measuring the zeta potential (ζ). The hydrodynamic diameter of the resulting suspensions of IONP:5-FU was determined by dynamic light scattering (DLS). A cytocompatibility analysis was performed using Caco-2 (human epithelial colorectal adenocarcinoma) cells. In this research, our goal was to find a relationship between the formulation ratio of nanoparticles and drug, and the cellular response after exposure, as a strategy to increase the efficacy of this drug-delivery system. The nanoparticle uptake and antitumor activity, including modulation of oxidative stress, apoptosis, and proliferation biomarkers, were analyzed. The present study showed that the nanoformulation with the ratio IONP:5-FU 1.5:1 had the highest anti-tumor efficiency. Moreover, decreased MCM-2 expression in Caco-2 cells exposed to dextran-coated iron oxide nanoparticles loaded with 5-FU was demonstrated for the first time.
Stressful Life Events, Unhealthy Eating Behaviors and Obesity among Chinese Government Employees: A Follow-Up Study
Authors: Qiu Dan , He Jun , Li Yilu , Ouyang Feiyun , Xiao Shuiyuan
Published: June 2023

Background: The underlying mechanisms of the relationship between stressful life events and obesity among Chinese workers are unclear. Objective: This study aimed to understand the processes and mechanisms involved in stressful life events, unhealthy eating behavior, and obesity among Chinese workers. Methods: From January 2018 to December 2019, a total of 15,921 government employees were included at baseline and they were followed-up until May 2021. Stressful life events were assessed using the Life Events Scale, and unhealthy eating behavior was assessed using four items. BMI was calculated as weight (kg) divided by height (m2) using physically measured data. Results: Overeating at each mealtime (OR = 2.21, 95%CI: 1.78–2.71) at baseline led to reports of higher risk of obesity at follow up. Eating before going to bed at night sometimes (OR = 1.51, 95%CI: 1.31–1.73) or often (OR = 3.04, 95%CI: 2.28–4.05) at baseline led to reports of higher risk of obesity at follow-up. Eating out sometimes (OR = 1.74, 95%CI: 1.47–2.07) or often (OR = 1.59, 95%CI: 1.07–2.36) at baseline led to reports of higher risk of obesity at follow-up. Stressful life events were not directly associated with obesity, but unhealthy eating behaviors, including overeating at each mealtime (β = 0.010, 95%CI: 0.007–0.014; β = 0.002, 95%CI: 0.001–0.004, respectively) and irregular meal timing (β = −0.011, 95%CI: −0.015–−0.008; β = −0.004, 95%CI: −0.006–−0.001, respectively), significantly mediated the associations between stressful life events at baseline and obesity at both baseline and follow-up. Conclusions: Unhealthy eating behaviors mediated the relationship between stressful life events and obesity. Interventions should be provided to workers who have experienced stressful life events and unhealthy eating behaviors.
ASF Vaccine Candidate ASFV-G-∆I177L Does Not Exhibit Residual Virulence in Long-Term Clinical Studies
Authors: Borca Manuel V. , Ramirez-Medina Elizabeth , Silva Ediane , Rai Ayushi , Espinoza Nallely , Velazquez-Salinas Lauro , Gladue Douglas P.
Published: June 2023

African swine fever (ASF) is an important disease in swine currently producing a pandemic affecting pig production worldwide. Except in Vietnam, where two vaccines were recently approved for controlled use in the field, no vaccine is commercially available for disease control. Up to now, the most effective vaccines developed are based on the use of live-attenuated viruses. Most of these promising vaccine candidates were developed by deleting virus genes involved in the process of viral pathogenesis and disease production. Therefore, these vaccine candidates were developed via the genomic modification of parental virus field strains, producing recombinant viruses and reducing or eliminating their residual virulence. In this scenario, it is critical to confirm the absence of any residual virulence in the vaccine candidate. This report describes the assessment of the presence of residual virulence in the ASFV vaccine candidate ASFV-G-∆I177L in clinical studies conducted under high virus loads and long-term observation periods. The results demonstrated that domestic pigs intramuscularly inoculated with 106 HAD50 of ASFV-G-∆I177L did not show the presence of any clinical sign associated with ASF when observed daily either 90 or 180 days after vaccination. In addition, necropsies conducted at the end of the experiment confirmed the absence of macroscopic internal lesions associated with the disease. These results corroborate the safety of using ASFV-G-∆I177L as a vaccine candidate.
Study of Oncolytic Virus Preservation and Formulation
Authors: Pan Lina , Liu Xiyu , Fan Dianfa , Qian Zhangbo , Sun Xinjun , Wu Pan , Zhong Liping
Published: June 2023

In recent years, oncolytic viruses (OVs) have emerged as an effective means of treating cancer. OVs have multiple oncotherapeutic functions including specifically infecting and lysing tumor cells, initiating immune cell death, attacking and destroying tumor angiogenesis and triggering a broad bystander effect. Oncolytic viruses have been used in clinical trials and clinical treatment as drugs for cancer therapy, and as a result, oncolytic viruses are required to have long-term storage stability for clinical use. In the clinical application of oncolytic viruses, formulation design plays a decisive role in the stability of the virus. Therefore, this paper reviews the degradation factors and their degradation mechanisms (pH, thermal stress, freeze–thaw damage, surface adsorption, oxidation, etc.) faced by oncolytic viruses during storage, and it discusses how to rationally add excipients for the degradation mechanisms to achieve the purpose of maintaining the long-term stability of oncolytic viral activity. Finally, the formulation strategies for the long-term formulation stability of oncolytic viruses are discussed in terms of buffers, permeation agents, cryoprotectants, surfactants, free radical scavengers, and bulking agent based on virus degradation mechanisms.
Chitosan-Based Thermogelling System for Nose-to-Brain Donepezil Delivery: Optimising Formulation Properties and Nasal Deposition Profile
Authors: Perkušić Mirna , Nižić Nodilo Laura , Ugrina Ivo , Špoljarić Drago , Jakobušić Brala Cvijeta , Pepić Ivan , Lovrić Jasmina , Safundžić Kučuk Maša , Trenkel Marie , Scherließ Regina , Zadravec Dijana , Kalogjera Livije , Hafner Anita
Published: June 2023

Donepezil nasal delivery strategies are being continuously investigated for advancing therapy in Alzheimer’s disease. The aim of this study was to develop a chitosan-based, donepezil-loaded thermogelling formulation tailored to meet all the requirements for efficient nose-to-brain delivery. A statistical design of the experiments was implemented for the optimisation of the formulation and/or administration parameters, with regard to formulation viscosity, gelling and spray properties, as well as its targeted nasal deposition within the 3D-printed nasal cavity model. The optimised formulation was further characterised in terms of stability, in vitro release, in vitro biocompatibility and permeability (using Calu-3 cells), ex vivo mucoadhesion (using porcine nasal mucosa), and in vivo irritability (using slug mucosal irritation assay). The applied research design resulted in the development of a sprayable donepezil delivery platform characterised by instant gelation at 34 °C and olfactory deposition reaching a remarkably high 71.8% of the applied dose. The optimised formulation showed prolonged drug release (t1/2 about 90 min), mucoadhesive behaviour, and reversible permeation enhancement, with a 20-fold increase in adhesion and a 1.5-fold increase in the apparent permeability coefficient in relation to the corresponding donepezil solution. The slug mucosal irritation assay demonstrated an acceptable irritability profile, indicating its potential for safe nasal delivery. It can be concluded that the developed thermogelling formulation showed great promise as an efficient donepezil brain-targeted delivery system. Furthermore, the formulation is worth investigating in vivo for final feasibility confirmation.
Pharmacogenetic Sex-Specific Effects of Methotrexate Response in Patients with Rheumatoid Arthritis
Authors: Ceballos Francisco C. , Chamizo-Carmona Eugenio , Mata-Martín Carmen , Carrasco-Cubero Carmen , Aznar-Sánchez Juan J. , Veroz-González Raúl , Rojas-Herrera Sara , Dorado Pedro , LLerena Adrián
Published: June 2023

Methotrexate (MTX) is a commonly used drug for the treatment of rheumatoid arthritis (RA), but its effectiveness can vary greatly among patients. Pharmacogenetics, the study of how genetic variations can affect drug response, has the potential to improve the personalized treatment of RA by identifying genetic markers that can predict a patient’s response to MTX. However, the field of MTX pharmacogenetics is still in its early stages and there is a lack of consistency among studies. This study aimed to identify genetic markers associated with MTX efficacy and toxicity in a large sample of RA patients, and to investigate the role of clinical covariates and sex-specific effects. Our results have identified an association of ITPA rs1127354 and ABCB1 rs1045642 with response to MTX, polymorphisms of FPGS rs1544105, GGH rs1800909, and MTHFR genes with disease remission, GGH rs1800909 and MTHFR rs1801131 polymorphisms with all adverse events, and ADA rs244076 and MTHFR rs1801131 and rs1801133, However, clinical covariates were more important factors to consider when building predictive models. These findings highlight the potential of pharmacogenetics to improve personalized treatment of RA, but also emphasize the need for further research to fully understand the complex mechanisms involved.
Anatomical Targeting of Anticancer Drugs to Solid Tumors Using Specific Administration Routes: Review
Authors: Saito Akira , Kitayama Joji , Nagai Ryozo , Aizawa Kenichi
Published: June 2023

Despite remarkable recent progress in developing anti-cancer agents, outcomes of patients with solid tumors remain unsatisfactory. In general, anti-cancer drugs are systemically administered through peripheral veins and delivered throughout the body. The major problem with systemic chemotherapy is insufficient uptake of intravenous (IV) drugs by targeted tumor tissue. Although dose escalation and treatment intensification have been attempted in order to increase regional concentrations of anti-tumor drugs, these approaches have produced only marginal benefits in terms of patient outcomes, while often damaging healthy organs. To overcome this problem, local administration of anti-cancer agents can yield markedly higher drug concentrations in tumor tissue with less systemic toxicity. This strategy is most commonly used for liver and brain tumors, as well as pleural or peritoneal malignancies. Although the concept is theoretically reasonable, survival benefits are still limited. This review summarizes clinical results and problems and discusses future directions of regional cancer therapy with local administration of chemotherapeutants.
Pueraria montana Population Structure and Genetic Diversity Based on Chloroplast Genome Data
Authors: Sun Jiahui , Wang Yiheng , Qiao Ping , Zhang Lei , Li Enze , Dong Wenpan , Zhao Yuping , Huang Luqi
Published: June 2023

Despite having a generally conserved structure, chloroplast genome data have been helpful for plant population genetics and evolution research. To mine Pueraria montana chloroplast genome variation architecture and phylogeny, we investigated the chloroplast variation architecture of 104 P. montana accessions from across China. P. montana’s chloroplast genome showed high diversity levels, with 1674 variations, including 1118 single nucleotide polymorphisms and 556 indels. The intergenic spacers, psbZ-trnS and ccsA-ndhD, are the two mutation hotspot regions in the P. montana chloroplast genome. Phylogenetic analysis based on the chloroplast genome dataset supported four P. montana clades. P. montana variations were conserved among and within clades, which showed high gene flow levels. Most P. montana clades were estimated to have diverged at 3.82–5.17 million years ago. Moreover, the East Asian summer monsoon and South Asian summer monsoon may have accelerated population divergence. Our results show that chloroplast genome sequences were highly variable and can be used as molecular markers to assess genetic variation and relationships in P. montana.
Anisotropy and Mechanical Properties of Nanoclay Filled, Medium-Density Rigid Polyurethane Foams Produced in a Sealed Mold, from Renewable Resources
Authors: Beverte Ilze , Cabulis Ugis , Andersons Janis , Kirpluks Mikelis , Skruls Vilis , Cabulis Peteris
Published: June 2023

Medium-density rigid polyurethane (PU) foams are often produced in sealed molds; therefore, the processes inside the mold and structure of the produced foam blocks need to be understood. The structural and mechanical anisotropy is shown to be the third variable along with (1) concentration of the nanoclay filler and (2) density, to determine the mechanical properties of the filled PU foam composites produced in a sealed mold. The varying anisotropy of the specimens hinders the accurate evaluation of the filling effect. The methodology for the estimation of the anisotropy characteristics of specimens from different locations within the nanoclay filled PU foam blocks is elaborated. A criterion, based on analysis of Poisson’s ratios, is formulated for the selection of specimens with similar anisotropy characteristics. The shear and bulk moduli are estimated theoretically, dependent on the filler’s concentration, using the experimentally determined constants.
The Everything-Is-a-Quantum-Wave Interpretation of Quantum Physics
Authors: Vedral Vlatko
Published: June 2023

In this paper, I would like to outline what I think is the most natural interpretation of quantum mechanics. By natural, I simply mean that it requires the least amount of excess baggage and that it is universal in the sense that it can be consistently applied to all the observed phenomena, including the universe as a whole. I call it the “Everything is a Quantum Wave” Interpretation (EQWI) because I think this is a more appropriate name than the Many Worlds Interpretation (MWI). The paper explains why this is so.
Diaconia and Identity: Agency of the Marginalised
Authors: Klaasen John Stephanus
Published: June 2023

The question under investigation relates to the agency of the marginalised as a model for taking responsibility for care and progress. This complex phenomenon of existence outside of the centre will be critically evaluated. Two notions of imago Dei—Wentzel’s image of God and Kelsey’s images of Christ—indicate the debates around the positioning of the marginalised. These debates are becoming an emerging field of interest within the development and diaconia fields of research. A growing interest is in the escalation and unprecedented poverty that some parts of the world are experiencing. The division amongst and between nations is growing rapidly, never seen in modern history. The interest of this contribution lies specifically within the intersections of identity and agency. I seek to explore Christian anthropology within and concerning the rest of God’s creation. Questions such as the absolute doctrinal formulation of what it means to be created in the image and likeness of God, how the doctrine relates to contemporary challenges such as the destructiveness of the created order by human beings, the oppressive and exploitative political and economic systems; and the continuation of the doctrine with situations of poverty will be considered. Using a post-colonial approach to Christian anthropology, I will argue that the marginalised as part of the creation of God has the call and gifts to take responsibility, the right and the dignity for care and development. Such an approach redefines marginalisation as a space of liberation. The positioning of the marginalised in relation to the centre and the agency of the poor will be correlated with Radford Ruether’s dialectical approach to what it means to be the church. Radford Ruether’s approach forms the basis for Dietrich’s view of the positioning of the marginalized within the diaconal perspective.
“We Are the Homeowners”: Sacred Textuality and the Social Structure of Jewish Religious Nationalism in Israel and the West Bank
Authors: Stern Nehemia
Published: June 2023

This article offers an anthropological look at sacred textuality by exploring the social and theological structure of Jewish religious nationalism in Israel and the West Bank. It argues that the study of sacred texts serves as a medium through which Jewish religious Zionists articulate what it means to return to an ancestral homeland. It demonstrates how the study of these sacred texts is implicated in the cultivation of two different structural modes through which religious Zionists relate to ideas of homecoming. On one side, homecoming rests in a revolutionary force of intellectual insight; on the other, it is expressed through the mystical and mysterious force of prophecy. In a broader sense, this article critiques the reticence of anthropologists to engage seriously with the broader theological ideas that are expressed through a textual medium that can be so much a part of the everyday experiences of individuals living in text-based societies.
Through Agnostic Eyes: Representations of Hinduism in the Cinema of Satyajit Ray
Authors: Sengoopta Chandak
Published: June 2023

Examining the filmmaker Satyajit Ray’s engagements with religious questions with reference to his films Devi (The Goddess), Mahapurush (The Holy Man), Ashani Sanket (Distant Thunder), Sadgati (Deliverance) and Ganashatru (A Public Enemy), this essay assesses the influence of Ray’s Brahmo inheritance, his personal atheism/agnosticism and his cultural fascination with Hinduism in his representations of women’s status and caste discrimination. It concludes that although Ray’s approach to Hinduism was far from one-dimensional or sectarian, its negative social consequences were emphasized more in his work than any positive role it might play in society and culture.
Use of ICEsat-2 and Sentinel-2 Open Data for the Derivation of Bathymetry in Shallow Waters: Case Studies in Sardinia and in the Venice Lagoon
Authors: Bernardis Massimo , Nardini Roberto , Apicella Lorenza , Demarte Maurizio , Guideri Matteo , Federici Bianca , Quarati Alfonso , De Martino Monica
Published: June 2023

Despite the high accuracy of conventional acoustic hydrographic systems, measurement of the seabed along coastal belts is still a complex problem due to the limitations arising from shallow water. In addition to traditional echo sounders, airborne LiDAR also suffers from high application costs, low efficiency, and limited coverage. On the other hand, remote sensing offers a practical alternative for the extraction of depth information, providing fast, reproducible, low-cost mapping over large areas to optimize and minimize fieldwork. Satellite-derived bathymetry (SDB) techniques have proven to be a promising alternative to supply shallow-water bathymetry data. However, this methodology is still limited since it usually requires in situ observations as control points for multispectral imagery calibration and bathymetric validation. In this context, this paper illustrates the potential for bathymetric derivation conducted entirely from open satellite data, without relying on in situ data collected using traditional methods. The SDB was performed using multispectral images from Sentinel-2 and bathymetric data collected by NASA’s ICESat-2 on two areas of relevant interest. To assess outcomes’ reliability, bathymetries extracted from ICESat-2 and derived from Sentinel-2 were compared with the updated and reliable data from the BathyDataBase of the Italian Hydrographic Institute.