bluuebunny/crossref_metadata_2025 huggingface dataset:
On the Recovery and Fatigue Life Extension of Stainless Steel 316 Metals by Means of Recovery Heat Treatment
Authors: Haghshenas Ali , Khonsari M. M.
Published: September 2020

In this paper, we propose a methodology for enhancing the fatigue life of SS316 by performing intermittent recovery heat-treatment (RHT) in the Argon environment at different temperatures. To this end, fully-reversed fatigue bending tests are conducted on the heat-treated SS316 specimens. Damping values are obtained using the impact excitation technique to assess the damage remaining in the material after each RHT and the corresponding fatigue life. Damping is also used to distinguish the three stages of the fatigue phenomenon and the onset of crack initiation. The results show that by performing intermittent RHTs, the density of dislocation is decreased substantially and fatigue life is improved. Examination of the damping results also reveals that the material becomes more brittle after the RHT due to the decrease in the density of dislocations. The fatigue life of the specimens is governed by these two phenomena.
Effect of Four Manufacturing Techniques (Casting, Laser Directed Energy Deposition, Milling and Selective Laser Melting) on Microstructural, Mechanical and Electrochemical Properties of Co-Cr Dental Alloys, Before and After PFM Firing Process
Authors: Barro Óscar , Arias-González Felipe , Lusquiños Fernando , Comesaña Rafael , del Val Jesús , Riveiro Antonio , Badaoui Aida , Gómez-Baño Félix , Pou Juan
Published: September 2020

The objective of this study was to compare four manufacturing processes of Co-Cr dental alloys: casting (CAST), computer aided design/computer aided manufacturing (CAD/CAM) milling (MILL), selective laser melting (SLM), and laser directed energy deposition (LDED). Comparison included microstructural, mechanical, and electrochemical analyses. Half of the samples obtained were heat treated to simulate the porcelain fused to metal (PFM) firing process, and the metal real state in an oral environment. Co-Cr dental alloys manufactured via casting, LDED, milling, and SLM techniques presented evident differences in their mechanical properties. However, their electrochemical performances were similar, with high resistance to corrosion in artificial saliva, in both aerated and deaerated media (corrosion rate under 4 microns per year). LDED and milling materials showed the highest modulus of toughness, and gave improved results in comparison with CAST and SLM techniques (p < 0.05). The LDED process could be implemented in the manufacturing of the restorative dental industry, with a high overall performance, competing directly with the best quality techniques, and reducing their disadvantages.
Effect of Disk Laser Beam Offset on the Microstructure and Mechanical Properties of Copper—AISI 304 Stainless Steel Dissimilar Metals Joints
Authors: Sahul Miroslav , Tomčíková Ema , Sahul Martin , Pašák Matej , Ludrovcová Barbora , Hodúlová Erika
Published: September 2020

Deoxidized oxygen free copper C12200, 1 mm in thickness, was welded to 1-mm thick AISI 304 stainless steel with disk laser. The butt-welded joints were produced with different welding parameters. Full factorial design of experiment (DoE) approach consisting of three factors and two levels was utilized. Laser powers used for welding were 1.3 and 1.9 kW and welding speeds of 20 and 30 mm/s. Two beam offsets were tested, namely, 100 μm toward copper side and 200 μm toward AISI 304 steel. It was found that beam offset possesses the largest influence on the welded joints’ tensile strength. Tensile strengths attained values more than 3.7 times higher in comparison to the AISI 304 steel beam offset. When lower laser power was used, the higher tensile strength was attained for copper sheet offset. Higher microhardness was observed when laser beam was offset to AISI 304 steel side. The average microhardness of the weld metal was higher than that of the weaker base material, copper sheet. Energy dispersive X-ray spectroscopy (EDS) analysis confirmed the heterogeneity in elemental composition across the welded joint interface, being lower when laser beam was offset to AISI 304 steel side. On the other hand, the copper content dropped to the average composition of weld metal at the distance of about 140 μm from copper-weld metal interface.
Mineral Inventory of the Algares 30-Level Adit, Aljustrel Mine, Iberian Pyrite Belt, Portugal
Authors: Silva Teresa P. , Matos João X. , De Oliveira Daniel , Veiga João P. , Morais Igor , Gonçalves Pedro , Albardeiro Luís
Published: September 2020

Mining activity in Algares (Aljustrel Mine, Portuguese sector of the Iberian Pyrite Belt, IPB) stems prior to Roman times. As the orebody is vertical and relatively thin, mining was carried out mainly along underground adits (galleries). Nowadays, the deposit is considered exhausted and the area is being rehabilitated for a different use. The Algares +30 level adit intersects two volcanic units of the IPB Volcano-Sedimentary Complex. The massive sulphide and related stockwork zone are hosted by the Mine Tuff volcanic unit and are exposed in the walls of the gallery, showing intense hydrothermal alteration. Along the mine adit, the geological sequence is affected by strong oxidation and supergene alteration, giving rise to the formation of secondary minerals through the oxidation of the sulphides. The most common minerals found were melanterite (FeSO4·7H2O) and chalcanthite (CuSO4·5H2O), forming essentially massive or crystalline aggregates, ranging from greenish to bluish colours. Melanterite from the walls revealed to be Cu-rich by opposition to that from stalactites/stalagmites formed below the old ore storage silo revealing the low-copper-grade ores exploited underground. The mineralogy of the efflorescent salts was used to ascertain the processes involved in their formation, and moreover, the inventory of minerals is presented, as well as their principal characteristics.
Provenance Analysis of the Paleogene Strata in the Northern Qaidam Basin, China: Evidences from Sediment Distribution, Heavy Mineral Assemblages and Detrital Zircon U‒Pb Geochronology
Authors: Yin Jianguo , Zhang Shuai , Wu Zhixiong
Published: September 2020

Using provenance analysis to build an accurate source-to-sink relationship is the key to infer mountain building scenarios around the Qaidam Basin, and also important to understanding the uplift and expansion of the Tibetan Plateau. However, some conflicting provenance inferences are caused by different interpretations for the prevalent existence of the late Paleozoic to early Mesozoic age group in detrital zircon U‒Pb age spectra of the Paleogene strata at the northern Qaidam Basin, and these need to be resolved. In this article, an integrated study of sediment distribution, heavy mineral assemblages, and detrital zircon U‒Pb geochronology is carried out to analyze provenance of the Paleogene strata at the northern Qaidam Basin. The decreasing trends of the net sand to gross thickness ratios and conglomerate percentages away from the Qilian Mountains and Altyn Tagh range to basin interior clearly support they are the provenance areas. Sedimentation of materials from the Altyn Tagh range is spatially confined to a small area in front of the mountains. A large sandy body with a uniform distribution of detrital zircon ages (containing a lot of the late Paleozoic to early Mesozoic zircon ages) and heavy mineral assemblages in the Xiaganchaigou Formation is supplied by the Qilian Mountains.
Engineered Multilayer Microcapsules Based on Polysaccharides Nanomaterials
Authors: Lombardo Salvatore , Villares Ana
Published: September 2020

The preparation of microcapsules composed by natural materials have received great attention, as they represent promising systems for the fabrication of micro-containers for controlled loading and release of active compounds, and for other applications. Using polysaccharides as the main materials is receiving increasing interest, as they constitute the main components of the plant cell wall, which represent an ideal platform to mimic for creating biocompatible systems with specific responsive properties. Several researchers have recently described methods for the preparation of microcapsules with various sizes and properties using cell wall polysaccharide nanomaterials. Researchers have focused mostly in using cellulose nanomaterials as structural components in a bio-mimetic approach, as cellulose constitutes the main structural component of the plant cell wall. In this review, we describe the microcapsules systems presented in the literature, focusing on the works where polysaccharide nanomaterials were used as the main structural components. We present the methods and the principles behind the preparation of these systems, and the interactions involved in stabilizing the structures. We show the specific and stimuli-responsive properties of the reported microcapsules, and we describe how these characteristics can be exploited for specific applications.
Protective Effects of Eicosapentaenoic Acid Plus Hydroxytyrosol Supplementation Against White Adipose Tissue Abnormalities in Mice Fed a High-Fat Diet
Authors: Illesca Paola , Valenzuela Rodrigo , Espinosa Alejandra , Echeverría Francisca , Soto-Alarcon Sandra , Campos Cristian , Rodriguez Alicia , Vargas Romina , Magrone Thea , Videla Luis A.
Published: September 2020

Objective: Obesity induced by high-fat diet (HFD) elicits white adipose tissue dysfunction. In this study, we have hypothesized that the metabolic modulator eicosapentaenoic acid (EPA) combined with the antioxidant hydroxytyrosol (HT) attenuates HFD-induced white adipose tissue (WAT) alterations. Methods: C57BL/6J mice were administered with a HFD (60% fat, 20% protein, 20% carbohydrates) or control diet (CD; 10% fat, 20% protein, 70% carbohydrates), with or without EPA (50 mg/kg/day), HT (5 mg/kg/day), or both for 12 weeks. Determinations in WAT include morphological parameters, EPA and docosahexaenoic acid content in phospholipids (gas chromatography), lipogenesis, oxidative stress (OS) and inflammation markers, and gene expression and activities of transcription factors, such as sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-gamma (PPAR-γ), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (p65 subunit) and nuclear factor erythroid 2-related factor 2 (Nrf2) (quantitative polymerase chain reaction and enzyme linked immunosorbent assay). Results: HFD led to WAT hypertrophy in relation to PPAR-γ downregulation. WAT metabolic dysfunction was characterized by upregulation of lipogenic SREBP-1c system, mitochondrial energy metabolism depression, loss of the antioxidant Nrf2 signaling with OS enhancement, n-3 long-chain polyunsaturated fatty acids depletion and activation of the pro-inflammatory NF-κB system. EPA and HT co-supplementation diminished HFD-dependent effects additively, reaching values close or similar to controls. Conclusion: Data presented strengthen the importance of combined protocols such as EPA plus HT to attenuate metabolic-inflammatory states triggered by obesity.
Indole Derivative Interacts with Estrogen Receptor Beta and Inhibits Human Ovarian Cancer Cell Growth
Authors: Verardi Laura , Fiori Jessica , Andrisano Vincenza , Locatelli Alessandra , Morigi Rita , Naldi Marina , Bertucci Carlo , Strocchi Elena , Boga Carla , Micheletti Gabriele , Calonghi Natalia
Published: September 2020

Ovarian cancer remains the leading cause of mortality among gynecological tumors. Estrogen receptor beta (ERβ) expression has been suggested to act as a tumor suppressor in epithelial ovarian cancer by reducing both tumor growth and metastasis. ERβ expression abnormalities represent a critical step in the development and progression of ovarian cancer: for these reasons, its re-expression by genetic engineering, as well as the use of targeted ERβ therapies, still constitute an important therapeutic approach. 3-{[2-chloro-1-(4-chlorobenzyl)-5-methoxy-6-methyl-1H-indol-3-yl]methylene}-5-hydroxy-6-methyl-1,3-dihydro-2H-indol-2-one, referred to here as compound 3, has been shown to have cytostatic as well cytotoxic effects on various hormone-dependent cancer cell lines. However, the mechanism of its anti-carcinogenic activity is not well understood. Here, we offer a possible explanation of such an effect in the human ovarian cancer cell line IGROV1. Chromatin binding protein assay and liquid chromatography mass spectrometry were exploited to localize and quantify compound 3 in cells. Molecular docking was used to prove compound 3 binding to ERβ. Mass spectrometry-based approaches were used to analyze histone post-translational modifications. Finally, gene expression analyses revealed a set of genes regulated by the ERβ/3 complex, namely CCND1, MYC, CDKN2A, and ESR2, providing possible molecular mechanisms that underline the observed antiproliferative effects.
Optimization of Plant Production by Seed Treatment in Two Wild Subspecies of Narcissus pseudonarcissus Rich in Alkaloids
Authors: Herranz Raquel , Copete Miguel A. , Herranz José M. , Copete Elena , Ferrandis Pablo
Published: September 2020

The daffodil Narcissus pseudonarcissus L. contains alkaloids of pharmaceutical interest. Wild daffodil populations have diverse genetic backgrounds and various genetic traits of possible importance. Developing protocols for plant production from seeds may ensure the availability of a large reservoir of individuals as well as being important for species with bulbs that are difficult to acquire. The closely related Narcissus pseudonarcissus subsp. munozii-garmendiae and subsp. nevadensis were investigated in this study because the alkaloids isolated from both are of high pharmacological interest. At the dispersal time, the seeds of both were dormant with underdeveloped embryos, i.e., morphophysiological dormancy (MPD). Experiments were conducted outdoors and under controlled laboratory conditions. Embryo growth and the percentages of radicle and seedling emergence were calculated under different temperature–light stratifications. In N. munozii-garmendiae, embryo growth occurred during warm stratification (28/14 °C or 25/10 °C) and the radicle then emerged when the temperature decreased, but the shoot was dormant. In N. nevadensis, the seeds germinated when cold stratified (5 °C) and then incubated at cool temperatures. Thus, N. munozii-garmendiae and N. nevadensis exhibit different levels of MPD, i.e., deep simple epicotyl and intermediate complex, respectively. Plant production protocols from seeds were established for both taxa in this study.
Bioactive Compounds and Health-Promoting Properties of Pear (Pyrus communis L.) Fruits
Authors: Kolniak-Ostek Joanna , Kłopotowska Dagmara , Rutkowski Krzysztof P. , Skorupińska Anna , Kruczyńska Dorota E.
Published: September 2020

Consuming food that is rich in antioxidants reduces the risk of developing chronic diseases and oxidative stress. Fruits and vegetables are an excellent source of substances with antioxidant and pro-health properties. Such raw materials, characterized by a high content of polyphenolic compounds and antioxidant capacity, include pear fruits. In this study, the concentrations of bioactive compounds, as well as the antioxidant, anti-inflammatory, and antiproliferative activity in fruits of five selected pear cultivars were determined and compared. LC–MS and UPLC–PDA methods were used to determine the polyphenolic, carotenoid, chlorophyll, and triterpenoid profiles and content, and the antioxidant activity was analyzed using DPPH and ferric-reducing ability of plasma (FRAP) tests. The anti-inflammatory activity was determined against COX-1 and COX-2 enzymes. The cytotoxic activity of the test compounds was assessed against six tumor cell lines. The results showed that the major group of phenolic compounds in all cultivars was phenolic acids. In the group of chromoplastic pigments, chlorophyllide a and 9-cis-β-carotene were the major compounds, while in the triterpene group, ursolic acid was dominant. The antioxidant potential correlated with the content of polyphenols and carotenoids, and was the strongest for the ‘Radana’ cultivar. The highest antiproliferative activity in all varieties was established for bladder cancer.
Synthesis of Natural-Like Snow by Ultrasonic Nebulization of Water: Morphology and Raman Characterization
Authors: Maggiore Ettore , Tommasini Matteo , Ossi Paolo M.
Published: September 2020

The current devices used to produce massive amounts of snow (i.e., snow machines) can be improved with concern to both the energy efficiency and the quality of snow. Here we investigate an alternative snow production method based on the ultrasonic nebulization of water and its subsequent condensation on the cold surfaces of a refrigerator. Inspection of the snow samples with a stereo optical microscope shows both dendritic and granular snow morphologies. The characterization of the samples by Raman spectroscopy shows a behavior consistent with that of a natural, low-density snow. Our results indicate that ultrasonic nebulization of water is an effective strategy for producing natural-like snow at the laboratory scale.
Benzophenone and Benzoylphloroglucinol Derivatives from Hypericum sampsonii with Anti-Inflammatory Mechanism of Otogirinin A
Authors: Huang Chun-Yi , Chang Tzu-Cheng , Wu Yu-Jing , Chen Yun , Chen Jih-Jung
Published: September 2020

Three new compounds, 4-geranyloxy-2-hydroxy-6-isoprenyloxybenzophenone (1), hypericumone A (2) and hypericumone B (3), were obtained from the aerial parts of Hypericum sampsonii, along with six known compounds (4–9). The structures of these compounds were determined through spectroscopic and MS analyses. Hypericumone A (2), sampsonione J (8) and otogirinin A (9) exhibited potent inhibition (IC50 values ≤ 40.32 μM) against lipopolysaccharide (LPS)-induced nitric oxide (NO) generation. Otogirinin A (9) possessed the highest inhibitory effect on NO production with IC50 value of 32.87 ± 1.60 μM. The well-known proinflammatory cytokine, tumor necrosis factor-alpha (TNF-α) was also inhibited by otogirinin A (9). Western blot results demonstrated that otogirinin A (9) downregulated the high expression of inducible nitric oxide synthase (iNOS). Further investigations on the mechanism showed that otogirinin A (9) blocked the phosphorylation of MAPK/JNK and IκBα, whereas it showed no effect on the phosphorylation of MAPKs/ERK and p38. In addition, otogirinin A (9) stimulated anti-inflammatory M2 phenotype by elevating the expression of arginase 1 and Krüppel-like factor 4 (KLF4). The above results suggested that otogirinin A (9) could be considered as potential compound for further development of NO production-targeted anti-inflammatory agent.
Synthesis of Vertical Carbon Nanotube Interconnect Structures Using CMOS-Compatible Catalysts
Authors: Ma Zichao , Zhou Shaolin , Zhou Changjian , Xiao Ying , Li Suwen , Chan Mansun
Published: September 2020

Synthesis of the vertically aligned carbon nanotubes (CNTs) using complementary metal-oxide-semiconductor (CMOS)-compatible methods is essential to integrate the CNT contact and interconnect to nanoscale devices and ultra-dense integrated nanoelectronics. However, the synthesis of high-density CNT array at low-temperature remains a challenging task. The advances in the low-temperature synthesis of high-density vertical CNT structures using CMOS-compatible methods are reviewed. Primarily, recent works on theoretical simulations and experimental characterizations of CNT growth emphasized the critical roles of catalyst design in reducing synthesis temperature and increasing CNT density. In particular, the approach of using multilayer catalyst film to generate the alloyed catalyst nanoparticle was found competent to improve the active catalyst nanoparticle formation and reduce the CNT growth temperature. With the multilayer catalyst, CNT arrays were directly grown on metals, oxides, and 2D materials. Moreover, the relations among the catalyst film thickness, CNT diameter, and wall number were surveyed, which provided potential strategies to control the tube density and the wall density of synthesized CNT array.
Formation of Free-Standing Inverse Opals with Gradient Pores
Authors: Hung Pei-Sung , Liao Chen-Hong , Huang Bo-Han , Chung Wei-An , Chang Shou-Yi , Wu Pu-Wei
Published: September 2020

We demonstrate the fabrication of free-standing inverse opals with gradient pores via a combination of electrophoresis and electroplating techniques. Our processing scheme starts with the preparation of multilayer colloidal crystals by conducting sequential electrophoresis with polystyrene (PS) microspheres in different sizes (300, 600, and 1000 nm). The critical factors affecting the stacking of individual colloidal crystals are discussed and relevant electrophoresis parameters are identified so the larger PS microspheres are assembled successively atop of smaller ones in an orderly manner. In total, we construct multilayer colloidal crystals with vertical stacking of microspheres in 300/600, 300/1000, and 300/600/1000 nm sequences. The inverse opals with gradient pores are produced by galvanostatic plating of Ni, followed by the selective removal of colloidal template. Images from scanning electron microscopy exhibit ideal multilayer close-packed structures with well-defined boundaries among different layers. Results from porometer analysis reveal the size of bottlenecks consistent with those of interconnected pore channels from inverse opals of smallest PS microspheres. Mechanical properties determined by nanoindentation tests indicate significant improvements for multilayer inverse opals as compared to those of conventional single-layer inverse opals.
Spectroscopic Evidence of Energy Transfer in BODIPY-Incorporated Nano-Porphyrinic Metal-Organic Frameworks
Authors: Seo Changwon , Kim Miyeon , Lee Jubok , Lee Chang Yeon , Kim Jeongyong
Published: September 2020

Metal–organic frameworks (MOFs) represent a class of solid-state hybrid compounds consisting of multitopic organic struts and metal-based nodes that are interconnected by coordination bonds, and they are ideal for light harvesting due to their highly ordered structure. These structures can be constructed with chromophore organic ligands structures for the purpose of efficient light harvesting. Here, we prepared porphyrin-based nano-scaled MOFs (nPCN-222) with BODIPY and I2BODIPY photosensitizers by incorporating BODIPY/I2BODIPY into nPCN-222 (nPCN-BDP/nPCN-I2BDP) and demonstrated resonance energy transfer from the donor (BODIPY/I2BODIPY) to the acceptor (nPCN-222) resulting in greatly enhanced fluorescence of nPCN-222, as visually manifested by time-resolved and space-resolved fluorescence imaging of the nano-scaled MOFs.
Characterization and Electrochemical Behaviour of Nanoscale Hydrotalcite-Like Compounds toward the Reduction of Nitrate
Authors: Li Liang , Yang Junhao , Yun Yafeng , Hu Shouxun , Huang Yuanxing
Published: September 2020

In this research, nano Cu/Al–HTLCs, Co/Al–HTLCs and Cu/Co/Al–HTLCs were synthesized, characterized, and tested in electrolytic reduction nitrate. Experimental results showed that Cu/Al–HTLCs were less stable than Co/Al–HTLCs due to the Jahn–Teller effect. However, electrocatalytic activity of copper was superior to that of cobalt; thus, Cu/Co/Al–HTLCs were selected based on their stable crystalline structure and electrochemical activity. The optimized Cu2CoAl–HTLC was highly active in nitrate reduction, with two peaks for nitrate and nitrite reduction, respectively. Ammonia, nitrite and N-containing gases were found to be the final products of constant potential electrolysis at −0.54 and −0.74 V.
Clinical Effects of Dietary Supplementation of Lutein with High Bio-Accessibility on Macular Pigment Optical Density and Contrast Sensitivity: A Randomized Double-Blind Placebo-Controlled Parallel-Group Comparison Trial
Authors: Machida Naomichi , Kosehira Marie , Kitaichi Nobuyoshi
Published: September 2020

Improvements in macular pigment optical density (MPOD) and contrast sensitivity after administration of 12 mg lutein alone and the timing at which efficacy is observed remain unknown. Therefore, lutein (12 mg), a crystalline formulation, was used in this study, considering its bioaccessibility. This study aimed to determine the effects of lutein administration for 16 weeks on MPOD, contrast sensitivity, and glare sensitivity, and changes in serum lutein levels were determined. The study subjects comprised 59 healthy male and female adults aged 20–69 years. The study diet included a placebo (placebo group) or a diet supplemented with 12 mg of lutein (lutein group). Each study diet was continuously administered for 16 weeks. At weeks 8 and 16, MPOD, contrast sensitivity, glare sensitivity, and serum lutein levels were evaluated. Compared with the placebo group, the lutein group showed significantly improved MPOD, contrast sensitivity, and glare sensitivity at week 16 and significantly increased serum lutein levels at weeks 8 and 16. Continuous administration of lutein for 16 weeks, considering its bioaccessibility, increased MPOD; it made the outlines of visible objects clearer and was effective in inhibiting decreases in visual function caused by glare from light.
How Bank Vole-PUUV Interactions Influence the Eco-Evolutionary Processes Driving Nephropathia Epidemica Epidemiology—An Experimental and Genomic Approach
Authors: Madrières Sarah , Tatard Caroline , Murri Séverine , Vulin Johann , Galan Maxime , Piry Sylvain , Pulido Coralie , Loiseau Anne , Artige Emmanuelle , Benoit Laure , Leménager Nicolas , Lakhdar Latifa , Charbonnel Nathalie , Marianneau Philippe , Castel Guillaume
Published: September 2020

In Europe, Puumala virus (PUUV) is responsible for nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS). Despite the presence of its reservoir, the bank vole, on most of French territory, the geographic distribution of NE cases is heterogeneous and NE endemic and non-endemic areas have been reported. In this study we analyzed whether bank vole-PUUV interactions could partly shape these epidemiological differences. We performed crossed-experimental infections using wild bank voles from French endemic (Ardennes) and non-endemic (Loiret) areas and two French PUUV strains isolated from these areas. The serological response and dynamics of PUUV infection were compared between the four cross-infection combinations. Due to logistical constraints, this study was based on a small number of animals. Based on this experimental design, we saw a stronger serological response and presence of PUUV in excretory organs (bladder) in bank voles infected with the PUUV endemic strain. Moreover, the within-host viral diversity in excretory organs seemed to be higher than in other non-excretory organs for the NE endemic cross-infection but not for the NE non-endemic cross-infection. Despite the small number of rodents included, our results showed that genetically different PUUV strains and in a lesser extent their interaction with sympatric bank voles, could affect virus replication and diversity. This could impact PUUV excretion/transmission between rodents and to humans and in turn at least partly shape NE epidemiology in France.
Species Detection within the Echinococcus granulosus sensu lato Complex by Novel Probe-Based Real-Time PCRs
Authors: Maksimov Pavlo , Bergmann Hannes , Wassermann Marion , Romig Thomas , Gottstein Bruno , Casulli Adriano , Conraths Franz J.
Published: September 2020

Infections with eggs of Echinococcus granulosus sensu lato (s.l.) can cause cystic echinococcosis in intermediate host animals and humans. Upon ingestion of viable eggs, oncospheres hatch from the eggs and subsequently develop into fluid-filled larval cysts, most frequently in the liver or the lungs. The slowly growing cysts progressively interfere with organ function. The risk of infection is determined by the host range of the parasite, its pathogenicity and other epidemiologically relevant parameters, which differ significantly among the five species within the E. granulosus s.l. complex. It is therefore essential to diagnose the correct species within E. granulosus s.l. to help understand specific disease epidemiology and to facilitate effective implementation of control measures. For this purpose, simple, fast and cost-effective typing techniques are needed. We developed quantitative real-time polymerase chain reactions (qPCRs) to target polymorphic regions in the mitochondrial genome of E. granulosus s.l. In a single-step typing approach, we distinguished E. granulosus s.l. members in four epidemiologically relevant subgroups. These were E. granulosus sensu stricto, E. equinus, E. ortleppi and the E. canadensis cluster. The technique also allowed identification and differentiation of these species from other Echinococcus or Taenia taxa for samples isolated from cysts or faeces.
Identification of Genes Involved in Antifungal Activity of Burkholderia seminalis Against Rhizoctonia solani Using Tn5 Transposon Mutation Method
Authors: Zhang Muchen , Wang Xiaoxuan , Ahmed Temoor , Liu Mengju , Wu Zhifeng , Luo Jinyan , Tian Ye , Jiang Hubiao , Wang Yanli , Sun Guochang , Li Bin
Published: September 2020

Rhizoctonia solani is the causative agent of rice sheath blight disease. In a previous study, we found that the growth of R. solani was inhibited by Burkholderia seminalis strain R456. Therefore, the present study was conducted to identify the genes involved in the antifungal activity of B. seminalis strain R456 by using a Tn5 transposon mutation method. Firstly, we constructed a random insertion transposon library of 997 mutants, out of which 11 mutants showed the defective antifungal activity against R. solani. Furthermore, the 10 antagonism-related genes were successfully identified based on analysis of the Tn5 transposon insertion site. Indeed, this result indicated that three mutants were inserted on an indigenous plasmid in which the same insertion site was observed in two mutants. In addition, the remaining eight mutants were inserted on different genes encoding glycosyl transferase, histone H1, nonribosomal peptide synthetase, methyltransferase, MnmG, sulfate export transporter, catalase/peroxidase HPI and CysD, respectively. Compared to the wild type, the 11 mutants showed a differential effect in bacteriological characteristics such as cell growth, biofilm formation and response to H2O2 stress, revealing the complexity of action mode of these antagonism-related genes. However, a significant reduction of cell motility was observed in the 11 mutants compared to the wild type. Therefore, it can be inferred that the antifungal mechanism of the 10 above-mentioned genes may be, at least partially, due to the weakness of cell motility. Overall, the result of this study will be helpful for us to understand the biocontrol mechanism of this bacterium.
Zoonotic and Public Health Implications of Campylobacter Species and Squamates (Lizards, Snakes and Amphisbaenians)
Authors: Masila Nicodemus M. , Ross Kirstin E. , Gardner Michael G. , Whiley Harriet
Published: September 2020

Campylobacter spp. is one of the most widespread infectious diseases of veterinary and public health significance. Globally, the incidence of campylobacteriosis has increased over the last decade in both developing and developed countries. Squamates (lizards, snakes and amphisbaenians) are a potential reservoir and source of transmission of campylobacteriosis to humans. This systematic review examined studies from the last 20 years that have reported squamate-associated human campylobacteriosis. It was found that C. fetus subsp. testudinum and C. fetus subsp. fetus were the most common species responsible for human campylobacteriosis from a squamate host. The common squamate hosts identified included bearded dragons (Pogona vitticeps), green iguana (Iguana iguana), western beaked gecko (Rhynchoedura ornate) and blotched blue-tongued skink (Tiliqua nigrolutea). People with underlying chronic illnesses, the immunocompromised and the elderly were identified as the most vulnerable population. Exposure to pet squamates, wild animals, consumption of reptilian cuisines and cross contamination with untreated water were risk factors associated with Campylobacter infections. Proper hand hygiene practices, responsible pet ownership, ‘One Health’ education and awareness on zoonotic diseases will help reduce the public health risks arising from Campylobacter exposure through squamates. Continued surveillance using molecular diagnostic methods will also enhance detection and response to squamate-linked campylobacteriosis.
Clinical and Laboratory Features of Three Rare Chinese V210I gCJD Patients
Authors: Xiao Kang , Zhou Wei , Gao Li-Ping , Wu Yue-Zhang , Wang Yuan , Chen Cao , Gao Chen , Shi Qi , Dong Xiao-Ping
Published: September 2020

Genetic human prion diseases are a group of inherited encephalopathies directly associated with different mutations in PrP-encoding gene PRNP, including more than 50 different mutations worldwide. Some genotypes of mutations show ethno-correlation, and among them, genetic Creutzfeldt–Jacob disease (gCJD) with V210I mutation is frequent in European countries but rare in East Asia. Here, we comparatively analyzed the clinical and laboratory features of three Chinese patients with V210I mutant identified via the Chinese National CJD Surveillance System (CNS-CJD) in 2019. Two cases were Han Chinese and one was Hui Chinese, without blood kinship. The onset ages of three cases were 69, 64, and 59 years old, respectively. The clinical features of V210I gCJD were similar to sporadic CJD (sCJD), displaying typical clinical symptoms and signs, except that Case 3 did not show myoclonic movement. All three cases displayed sCJD-associated abnormalities on MRI and positive CSF 14-3-3, while two cases recorded typical EEG abnormalities. Only one case was positive in CSF real-time quaking-induced conversion (RT-QuIC). Appearances of mutism in three cases were relatively fast, with the intervals of 30 to 50 days after onset. Family history was not reported in all three cases. Those V210I gCJD cases are rare in China, and probably the first three in East Asia.
Essential Oils of Zingiber Species from Vietnam: Chemical Compositions and Biological Activities
Authors: Huong Le Thi , Chung Nguyen Thanh , Huong Trinh Thi , Sam Ly Ngoc , Hung Nguyen Huy , Ogunwande Isiaka Ajani , Dai Do Ngoc , Linh Le Duy , Setzer William N.
Published: September 2020

Mosquito-borne diseases are a large problem in Vietnam as elsewhere. Due to environmental concerns regarding the use of synthetic insecticides as well as developing insecticidal resistance, there is a need for environmentally-benign alternative mosquito control agents. In addition, resistance of pathogenic microorganisms to antibiotics is an increasing problem. As part of a program to identify essential oils as alternative larvicidal and antimicrobial agents, the leaf, stem, and rhizome essential oils of several Zingiber species, obtained from wild-growing specimens in northern Vietnam, were acquired by hydrodistillation and investigated using gas chromatography. The mosquito larvicidal activities of the essential oils were assessed against Culex quinquefasciatus, Aedes albopictus, and Ae. aegypti, and for antibacterial activity against a selection of Gram-positive and Gram-negative bacteria, and for activity against Candida albicans. Zingiber essential oils rich in α-pinene and β-pinene showed the best larvicidal activity. Zingiber nudicarpum rhizome essential oil showed excellent antibacterial activity against Enterococcus faecalis, Staphylococcus aureus, and Bacillus cereus, with minimum inhibitory concentrations (MIC) of 2, 8, and 1 μg/mL, respectively. However, the major components, α-pinene and β-pinene, cannot explain the antibacterial activities obtained.
Achene Morphology and Anatomy of Clematis L. (Ranunculaceae) in Korea and Its Taxonomic Implications
Authors: Ghimire Balkrishna , Park Beom Kyun , Son Dong Chan , Oh Seung-Hwan
Published: September 2020

Clematis, a widely distributed genus in Ranunculaceae, is one of the most difficult groups of taxa in the family from a taxonomic point of view. A comprehensive study on achene morphology and the anatomy of 19 taxa of Clematis from Korea was carried out using scanning electron and light microscopy to evaluate the taxonomic significance of achene characters. Clematis achenes are elliptical, obovate or fusiform in shape, light yellow or brown to black in color and completely or sparsely covered with hairs. The permanent style is elongated and plumose in all the studied taxa except C. brachyura. We found that the size, indument, permanent style, surface sculpture, shape in cross-section, and nature and thickness of the exocarp, and endocarp were valuable achene features for species delimitation and may contribute to the unraveling of the taxonomic problems in the genus Clematis. One-way analysis of variance (ANOVA) indicated that the quantitative achene variables among the species were highly significant (p < 0.001). Principal component analyses based on seven quantitative characters and UPGMA (unweighted pair-group method with arithmetic mean) analysis based on seven quantitative and 18 qualitative characters also signify the utility of achene features for taxonomic discriminations of the Clematis taxa within the genus. Similar to other morphological characters in the genus Clematis, achene morphological and anatomical characters with the limited taxonomic value alone cannot be expected to resolve the infrageneric relationships but certain achene features combined with other morphological features could be useful as an alternative means of determining the infrageneric relationships within the genus.
Disposal of Personal Protective Equipment during the COVID-19 Pandemic Is a Challenge for Waste Collection Companies and Society: A Case Study in Poland
Authors: Nowakowski Piotr , Kuśnierz Sandra , Sosna Patrycja , Mauer Jakub , Maj Dawid
Published: September 2020

One of the social measures applied during the COVID-19 pandemic has been the use of personal protective equipment (PPE)—face masks and gloves. As a result, this waste category has expanded enormously. This study investigates waste management issues from multiple perspectives, including local governments, waste collection companies, and individual citizens in Poland using a telephone survey for institutions and an online questionnaire for individuals. The results of this study show that approximately 80% of local governments in the Silesian region have applied special measures for handling and collection of waste PPE. Only 13% of waste collection companies have applied special collection schedules for the waste generated at quarantine collection points due to the high costs of changing collection schedules, providing additional vehicles, and paying for more labor. The information campaigns focusing on new methods of PPE waste collection have been difficult to introduce on a large scale, and citizens need better information regarding how to handle and dispose of waste PPE. Results indicated the most helpful method in supporting waste PPE collection would be automatic PPE dispensers with waste PPE collection options and waste bags of a designated color. The respondents identified waste PPE pollution of the environment as an issue and the necessity for proper recovery of this waste stream.
Robotic Systems of Systems Based on a Decentralized Service-Oriented Architecture
Authors: Siefke Lennart , Sommer Volker , Wudka Björn , Thomas Carsten
Published: September 2020

Multi-robot systems are often static and pre-configured during the design time of their software. Emerging cooperation between unknown robots is still rare and limited. Such cooperation might be basic like sharing sensor data or complex like conjoined motion planning and acting. Robots should be able to detect other robots and their abilities during runtime. When cooperation seems to be possible and beneficial, it should be initiated autonomously. A centralized cloud control shall be avoided. Using software patterns belonging to service-oriented architectures, the robots are able to discover other robots and their abilities during runtime. These abilities are implemented as services and described by their interfaces. Composition of services can be done easily and flexibly. The software patterns originally belonging to cloud computing could be successfully adopted to decentralized multi-robot systems. The developed concept allows autonomous systems to cooperate flexibly and to compose multi-robot systems during runtime.
The Effects of Long-Term Child–Robot Interaction on the Attention and the Engagement of Children with Autism
Authors: van Otterdijk Maria , de Korte Manon , van den Berk-Smeekens Iris , Hendrix Jorien , van Dongen-Boomsma Martine , den Boer Jenny , Buitelaar Jan , Lourens Tino , Glennon Jeffrey , Staal Wouter , Barakova Emilia
Published: September 2020

Using a social robot has been proven to have multiple benefits for the training of children with Autism Spectrum Disorder (ASD). However, there is no clarity on the impact of the interaction quality between a child with ASD and a robot on the effectiveness of the therapy. Previous research showed that the use of a robot in Pivotal Response Treatment (PRT) could be an effective treatment component in diminishing ASD-related symptoms. Further analyzing the data from a randomized controlled trial of PRT treatment, we looked at the long-term effects of child–robot game interactions to see whether the interaction quality changes over time. The attention and the engagement of six children were measured through the observation of non-verbal behavior at three different stages in the treatment that took 20 sessions per child. The gaze and arm/hand behavior of the participants towards the robot, the game, and other present humans were observed. The analysis showed no significant decrease in the attention and the engagement of the children towards the robot and the game. However, the attention and engagement toward the parents of the children increased. We conclude that the main result of sustained attention and engagement with the robot is due to the personalization of the games to meet the specific needs of this user group. These specific needs are met through inclusion of variability to the level of development and personal choice of each participating child. We see the additional finding of increased attention towards the parents as especially positive since the children are expected to improve in human–human interaction as a result of this treatment.
New Uses for the Personal Glucose Meter: Detection of Nucleic Acid Biomarkers for Prostate Cancer Screening
Authors: Abardía-Serrano Clara , Miranda-Castro Rebeca , de-los-Santos-Álvarez Noemí , Lobo-Castañón María Jesús
Published: September 2020

A personal glucose meter (PGM)-based method for quantitative detection of a urinary nucleic acid biomarker in prostate cancer screening, the so-called PCA3, is reported herein. A sandwich-type genoassay is conducted on magnetic beads to collect the target from the sample by specific hybridization, making the assay appropriate for PCA3 detection in biological fluids. The success of the method hinges on the use of alkaline phosphatase (ALP) to link the amount of nucleic acid biomarker to the generation of glucose. In particular, specifically attached ALP molecules hydrolyze D-glucose-1-phosphate into D-glucose, thus enabling the amplification of the recorded signal on the personal glucose meter. The developed genoassay exhibits good sensitivity (3.3 ± 0.2 mg glucose dL−1 pM−1) for PCA3, with a dynamic range of 5 to 100 pM and a quantification limit of 5 pM. Likewise, it facilitates point-of-care testing of nucleic acid biomarkers by using off-the-shelf PGM instead of complex instrumentation involved in traditional laboratory-based tests.
Research on Detection Mechanism of Weld Defects of Carbon Steel Plate Based on Orthogonal Axial Eddy Current Probe
Authors: Huang Linnan , Liao Chunhui , Song Xiaochun , Chen Tao , Zhang Xu , Deng Zhiyang
Published: September 2020

The uneven surface of the weld seam makes eddy current testing more susceptible to the lift-off effect of the probe. Therefore, the defect of carbon steel plate welds has always been a difficult problem in eddy current testing. This study aimed to design a new type of eddy current orthogonal axial probe and establish the finite element simulation model of the probe. The effect of the probe structure, coil turns, and coil size on the detection sensitivity was simulated. Further, a designed orthogonal axial probe was used to conduct a systematic experiment on the weld of carbon steel specimens, and the 0.2 mm width and 1 mm depth of weld defects of carbon steel plates were effectively detected. The experimental results showed that the new orthogonal axial eddy current probe effectively suppressed the unevenness effect of the weld surface on the lift-off effect during the detection process.
A Robot Hybrid Hierarchical Network for Sensing Environmental Variables of a Smart Grid
Authors: Liu Jiayang , Wu Gongping , Fan Fei , Li Yuxin
Published: September 2020

With the rapid development of the social economy, high-voltage transmission lines as power supply infrastructure are increasing, subsequently presenting a new challenge to the effective monitoring of transmission lines. The dynamic sensor network integrated with robots can effectively solve the elastic monitoring of transmission lines, but the problems of real-time performance, energy consumption and economy of the network need to be solved. To solve this problem, a dynamic network deployment method based on the hybrid hierarchical network (HHN) is proposed to realize a low-cost, energy-saving and real-time dynamic sensing system for overhead high-voltage transmission lines. Through case analysis and simulation, combined with the vague set multi-attribute decision-making method (MADM) with scheme preference, the network deployment and optimization results under multi-parameter constraints are obtained.
Grating-Corner-Cube-Based Roll Angle Sensor
Authors: Zhou Siyu , Le Vunam , Mi Qinggai , Wu Guanhao
Published: September 2020

This paper presents a specifically designed grating-corner-cube sensor for precise roll angle measurements. Owing to the diffraction characteristics of the transmission grating and reflection characteristics of the corner cube, two spatially separated parallel beams are naturally constructed. Through differential detection of the positions of two parallel beams, we experimentally demonstrate the possibility of a precise roll angle measurement at a high refresh rate. A performance evaluation of the proposed technique indicates a stability of 0.46 arcsec over 5 min. Compared with a commercial autocollimator over a range of 500 arcsec, the residuals are maintained within ±2 arcsec with a standard deviation of 1.37 arcsec. Furthermore, a resolution of 0.8 arcsec can be achieved using the proposed method. The developed compact roll angle sensor has potential applications in academic and industrial fields.
Method for Translation and Rotation Decoupling of Test Mass in Full-Maglev Vertical Superconducting Gravity Instruments
Authors: Wang Lulu , Chen Daiyong , Liu Xikai , Chen Liang , Liu Xiangdong
Published: September 2020

For full-maglev vertical superconducting gravity instruments, displacement control in the non-sensitive axis is a key technique to suppress cross-coupling noise in a dynamic environment. Motion decoupling of the test mass is crucial for the control design. In practice, when levitated, the test mass is always in tilt, and unknown parameters will be introduced to the scale factors of displacement detection, which makes motion decoupling work extremely difficult. This paper proposes a method for decoupling the translation and rotation of the test mass in the non-sensitive axis for full-maglev vertical superconducting gravity instruments. In the method, superconducting circuits at low temperature and adjustable gain amplifiers at room temperature are combined to measure the difference between the scale factors caused by the tilt of the test mass. With the measured difference of the scale factors, the translation and rotation are decoupled according to the theoretical model. This method was verified with a test of a home-made full-maglev vertical superconducting accelerometer in which the translation and rotation were decoupled.