bluuebunny/crossref_metadata_2025 huggingface dataset:
Multicenter Evaluation of Circulating Plasma MicroRNA Extraction Technologies for the Development of Clinically Feasible Reverse Transcription Quantitative PCR and Next-Generation Sequencing Analytical Work Flows
Authors: Kloten Vera , Neumann Martin H D , Di Pasquale Francesca , Sprenger-Haussels Markus , Shaffer Jonathan M , Schlumpberger Martin , Herdean Andrei , Betsou Fay , Ammerlaan Wim , af Hällström Taija , Serkkola Elina , Forsman Tarja , Lianidou Evi , Sjöback Robert , Kubista Mikael , Bender Sebastian , Lampignano Rita , Krahn Thomas , Schlange Thomas ,   for the CANCER-ID consortium
Published: June 2019

BACKGROUND

In human body fluids, microRNA (miRNA) can be found as circulating cell-free miRNA (cfmiRNA), as well as secreted into extracellular vesicles (EVmiRNA). miRNAs are being intensively evaluated as minimally invasive liquid biopsy biomarkers in patients with cancer. The growing interest in developing clinical assays for circulating miRNA necessitates careful consideration of confounding effects of preanalytical and analytical parameters.

METHODS

By using reverse transcription quantitative real-time PCR and next-generation sequencing (NGS), we compared extraction efficiencies of 5 different protocols for cfmiRNA and 2 protocols for EVmiRNA isolation in a multicentric manner. The efficiency of the different extraction methods was evaluated by measuring exogenously spiked cel-miR-39 and 6 targeted miRNAs in plasma from 20 healthy individuals.

RESULTS

There were significant differences between the tested methods. Although column-based extraction methods were highly effective for the isolation of endogenous miRNA, phenol extraction combined with column-based miRNA purification and ultracentrifugation resulted in lower quality and quantity of isolated miRNA. Among all extraction methods, the ubiquitously expressed miR-16 was represented with high abundance when compared with other targeted miRNAs. In addition, the use of miR-16 as an endogenous control for normalization of quantification cycle values resulted in a decreased variability of column-based cfmiRNA extraction methods. Cluster analysis of normalized NGS counts clearly indicated a method-dependent bias.

CONCLUSIONS

The choice of plasma miRNA extraction methods affects the selection of potential miRNA marker candidates and mechanistic interpretation of results, which should be done with caution, particularly across studies using different protocols.
Effects of rations containing formaldehyde-protected soybean meal on meat production in Kacang goats
Authors: Adiwinarti Retno , Budisatria I. Gede Suparta , Kustantinah K. , Rusman R. , Indarto Edwin
Published: June 2019

Aim: This study aimed to investigate effects of rations containing formaldehyde-protected soybean meal on meat production in Kacang goats. Materials and Methods: Fourteen yearling Kacang bucks, weighing 15.8-19.8 kg, were arranged in a completely randomized design. The treatments included a control (PSBM0): 100% untreated SBM; PSBM50: 50% untreated SBM + 50% formaldehyde-protected SBM; and PSBM100: 100% formaldehyde-protected SBM. Results: The goats disliked the protected SBM. Therefore, differences in their intakes were reflected in their average daily gain (ADG). The ADG and slaughtered weight of the control group were the highest, while those of the PSBM100 and PSBM50 groups were similar. The carcass weights and meat production of the control group were higher than those of the PSBM50 group, but the retained protein to the meat conversion ratio of the PSBM50 group was lower than that of the control. The carcass percentages were similar between the treatments. Conclusion: The retained protein to meat conversion ratio of Kacang goats fed with 50% formaldehyde-protected SBM showed the lowest value, indicating that these rations efficiently produced meat in the carcass.
Взаимоотношения церковных и светских властей в Сибири XVII столетия в трудах Н.Н. Оглоблина
Authors: Силаева Ирина Александровна
Published: June 2019

Известный российский историк и архивист конца XIX — начала XX в. Н.Н. Оглоблин по ряду документов архива Сибирского приказа в статье «Дело о самовольном приезде в Москву тобольского архиепископа Симеона, в 1661 г.» и третьей части фундаментального труда «Обозрение столбцов и книг Сибирского приказа (1592-1768 гг.)» проанализировал обстоятельства многочисленных конфликтов между несколькими представителями воеводской и архиерейской властей в Азиатской России XVII столетия. Особенно острыми такие конфликты были при архиепископе Симеоне и управлявшими в то время Тобольским разрядом стольником князем А.И. Буйносовым-Ростовским и боярином князем И.А. Хилковым.

Причинами вражды Н.Н. Оглоблин считал злоупотребления воевод, прежде всего их действия, направленные на подчинение духовной власти светской.

Как выяснил ученый, архиепископ Симеон самовольно, не получив на то обязательного разрешения царя Алексея Михайловича, посетил Москву, чтобы разоблачить произвол тобольских воевод, прежде всего боярина князя И.А. Хилкова. Святитель, получив всего одну аудиенцию у государя, добился лишь удовлетворения просьб относительно материального обеспечения Софийского дома и возврата крестьян на принадлежавшие ему земли.

По данным историка, хотя тобольские архиепископы, особенно Симеон, обращались к государю с челобитными, чтобы доказать свою правоту в спорах с воеводами и ограничить своеволие разрядных администраторов, зачастую жалобы владык оставались безрезультатными из-за отсутствия у них покровителей в Москве, а также политики верховной власти, все более, главным образом с середины XVII в., пытавшейся расширить прерогативы своих наместников в Сибири.
Entrevista com Mario Tanomaru Filho
Authors: Tanomaru Filho Mario , Alcalde Murilo Priori
Published: June 2019

» Graduado em Odontologia pela USP, Faculdade de Odontologia de Bauru (1987).
» Mestre em Endodontia pela USP, Faculdade de Odontologia de Bauru (1992).
» Professor Assistente da UNESP, Faculdade de Odontologia de Araraquara, Disciplina de Endodontia, desde 1992.
» Doutor em Endodontia pela UNESP, Faculdade de Odontologia de Araraquara (1996).
» Livre-Docente em Endodontia pela UNESP, Faculdade de Odontologia de Araraquara (2001).
» Professor Associado da UNESP, Faculdade de Odontologia de Araraquara, Disciplina de Endodontia, desde 2001.
» Professor Titular em Endodontia pela UNESP, Faculdade de Odontologia de Araraquara, Disciplina de Endodontia, desde 2010.
» Coordenador do Programa de Pós-Graduação em Odontologia da UNESP, Faculdade de Odontologia de Araraquara (2010-2012).
» Coordenador do Curso de Especialização em Endodontia da UNESP, Faculdade de Odontologia de Araraquara, desde 2016.
» Bolsista de Produtividade em Pesquisa CNPq (PQ 1C).
Efeito de diferentes protocolos de irrigação final na remoção da smear layer
Authors: Lima Carolina Oliveira de , Carvalho Fernanda Leal Fonseca de , Fidel Sandra Rivera , Campos Celso Neiva , Marion Jefferson Jose de Carvalho , Fidel Rivail Antonio Sergio , Prado Maíra do
Published: June 2019

Objetivo: esse estudo avaliou o efeito de protocolos de irrigação final, associados ao uso de EDTA e QMix, na remoção de smear layer. Métodos: foram utilizados 40 dentes humanos com canal único. Os canais foram instrumentados com sistema ProTaper Universal SX-F3. As substâncias químicas utilizadas durante o preparo foram hipoclorito de sódio a 5,25% (NaOCl) ou clorexidina a 2% (CHX) gel. Terminado o preparo, os dentes foram divididos em oito grupos, de acordo com os irrigantes: G1) NaOCl 5,25% + EDTA 17% + NaOCl 5,25%; G2) NaOCl 5,25% + EDTA 17% + água destilada (AD); G3) NaOCl 5,25% + QMix + NaOCl 5,25%; G4) NaOCl 5,25% + QMix + AD; G5) CHX 2% gel + EDTA 17% + CHX 2% solução; G6) CHX 2% gel + EDTA 17% + AD; G7) CHX 2% gel + QMix + CHX 2% solução e G8) CHX 2% gel + QMix + AD. As amostras foram avaliadas por microscopia eletrônica de varredura ambiental, antes e após os protocolos de irrigação final. As imagens foram classificadas por escores, em relação à presença de smear layer, e os dados foram analisados pelos testes de Kruskal-Wallis e de Mann-Whitney. Resultados: o G1 mostrou os melhores resultados em relação à remoção de smear layer (p < 0,05). A CHX gel 2% mostrou menor formação de smear layer, quando comparada ao NaOCl, após o preparo biomecânico; e o NaOCl apresentou melhor limpeza como irrigante final (p < 0,05). Conclusões: o NaOCl como irrigante final, combinado ao EDTA, mostrou melhor capacidade de remoção da smear layer.
Estudo in vitro da morfologia dos canais radiculares do primeiro pré-molar inferior, utilizando tomografia computadorizada de feixe cônico
Authors: Aguiar Francisco Jose Nunes , Lopes Matheus Araujo Brito Santos , Pierote Josue Junior Araujo , Delboni Maraisa Greggio
Published: June 2019

Introdução: o tratamento endodôntico objetiva limpar e modelar o sistema de canais radiculares. O preparo químico-mecânico e a obturação tridimensional (3D) do sistema de canais radiculares são baseados no conhecimento da anatomia dentária interna. As radiografias convencionais e periapicais digitais apresentam limitações na definição da imagem, devido à anatomia 3D do elemento a ser tratado, que é compactada em uma imagem bidimensional. A tomografia computadorizada de feixe cônico (TCFC) é, atualmente, um interessante instrumento para análises anatômicas, devido à precisão volumétrica e ausência de sobreposição de estruturas. Métodos: foram analisados, in vitro, sessenta e quatro (n = 64) primeiros pré-molares inferiores humanos extraídos por indicação terapêutica. Em seguida, foram feitos protótipos com gengiva artificial de silicone, posicionando-se os elementos de forma semelhante a arcadas, para serem geradas as imagens de TCFC e analisadas por um endodontista experiente. O número de canais por raiz foi quantificado em cada elemento dentário e suas configurações anatômicas foram descritas seguindo a classificação de Vertucci. Resultados: foram encontrados 59% de canais Tipo I (n = 38); 0% do Tipo II (n = 0); 9% do Tipo III (n = 6); 6% do Tipo IV (n = 4); 16% do Tipo V (n = 10); 3% do Tipo VI (n = 2); 5% do Tipo VII (n = 3); 0% do Tipo VIII (n = 0) e 2% do Tipo C-shaped. Conclusão: as imagens de TCFC revelam em 3D a anatomia dentária interna, permitindo o planejamento da terapia endodôntica e um mapeamento detalhado da morfologia interna. O conhecimento dessa microanatomia viabiliza uma maior previsibilidade na realização do tratamento, assim como no seu prognóstico, e possibilita melhorar a descontaminação de áreas anatômicas complexas.
Effectiveness of force dynamic explanations of English causative verbs and the role of imagery
Authors: Mueller Charles M. , Tsushima Yasuhiro
Published: June 2019

The current study examines the effectiveness of a CL-based force dynamic (FD) lesson relative to a more conventional approach that seeks to convey the target semantics through corresponding L1 forms. Exp. 1 (N=67) examined Japanese EFL students’ acquisition of the English verbsforce, get, have, help, let, make, andprevent, comparing the effectiveness of force dynamic explanations with dynamic diagrams and a translation-based approach. Both groups showed significant learning on both the posttest and delayed posttest, but the FD group’s greater gains over the conventional instruction on both the posttest and delayed posttest did not reach significance. Exp. 2 (N=97) replicated most of the Exp. 1 conditions but with a slightly altered instructional format to ensure participant’s focus on force dynamic relationships. The FD group’s greater gains on a posttest given three weeks following instruction fell short of statistical significance. Exp. 3 (N=54) compared the effectiveness of a FD lesson using dynamic images with a lesson without such images and found no significant differences between the groups. The three experiments demonstrate that CL-based instruction on force dynamics provides a viable alternative to conventional instruction in which target semantics are conveyed through translation.
Public television and anti-immigrant sentiments in Europe. A multilevel analysis of patterns in television consumption
Authors: Jacobs Laura , Hooghe Marc
Published: June 2019

Mass media have been accused of cultivating anti-immigrant sentiments in Western societies. Most studies on this topic, however, have not made a distinction between the types of television program (information vs. entertainment) or television station (public vs. commercial). Adopting a comparative approach, we use data from the six waves of the European Social Survey (ESS, 2002–2012, n = 162,987) to assess the relationship between individual and aggregate level patterns of television consumption and anti-immigrant sentiments in European societies. Individual television viewing time is positively associated with anti-immigrant sentiments, while frequent exposure to news and information programs is associated with lower levels of anti-immigrant sentiments. At the aggregate level we observe a positive effect of the total viewing time in society on anti-immigrant sentiments. In the conclusion we offer some suggestions on how this effect could be explained.
Labeling for linearization
Authors: Takita Kensuke
Published: June 2019

The primary goal of the present paper is to argue for the hypothesis that labeling is required for linearization, which is calledLabeling for Linearization(LfL). To achieve this goal, it is first argued that labels are not necessary for semantic interpretation. It is then proposed that labels are necessary for linearization at the PF-interface in that they serve as a device to encode structural asymmetries that are employed to determine precedence relations, which are asymmetric as well. It is also shown thatLfLcan remove several problems of the original labeling framework. Building on the idea that Spell-Out applies to the whole phase but not its subpart, it is illustrated that theLfL-based analysis can solve the problem concerning the variable ways of applying Spell-Out, which arises in the standard phase theory. Extending theLfL-based framework to Japanese, a novel analysis of particle-stranding ellipsis is also proposed. Incorporating some insights of recent approaches that particle-stranding ellipsis arises through a PF-deletion process, it is shown that the proposed analysis based onLfLoffers a theoretically more suitable characterization of the PF-deletion process. In this way, the present article contributes to not only sharpening the core theoretical notions regarding structure building and linearization in terms of labeling but also deepening our understanding of the structure of Japanese.
Effects of pre-ozonation and chemical coagulation on the removal of turbidity, color, TOC, and chlorophyll a from drinking water
Authors: Masoomi Bahman , Jaafarzadeh Neamatollah , Tabatabaie Tayebeh , Kouhgardi Esmaeil , Jorfi Sahand
Published: June 2019

Background: Ozone can be used as a single technology or in combination with other processes to improve the coagulation- flocculation or biodegradability in order to remove pollutants in natural water treatment. Methods: In this study, the effects of pre-ozonation with coagulant substances on the quality parameters of drinking water were investigated using humic acid, kaolin, clay, and green algae in a pilot scale. This study was conducted under laboratory conditions (at both acidic and alkaline pH in different dosages of ozone and coagulant at ozone contact time with simulated water sample (5-20 minutes) in different scenarios). Results: The highest removal efficiency of parameters in the state of pre-ozonation alone and preozonation with a coagulant was observed at contact time of 20 minutes, ozone dosage of 5 g/h, coagulant dosage of 25 mg/L, at alkaline pH along with a decrease in temperature. So that, the average removal rate of turbidity, total organic carbon (TOC), color, and chlorophyll a in contact time of 20 minutes was 76.9%, 52.8%, 66.6%, and 85%, respectively. However, compared to ozonation under similar conditions, the reduction in turbidity, TOC, color, and chlorophyll a was 36.13%, 24.4%, 32.13%, and 79.6%, respectively. Also, it was revealed that pre-ozonation with coagulant could effectively improve the removal of parameters. Conclusion: However, since pre-ozonation can be effectively used to improve the coagulation efficacy in the drinking water treatment, the pre-ozonation combined with coagulation is proposed as an alternative to conventional coagulation to improve the process of drinking water treatment plant.
C-reactive protein (CRP) promotes malignant properties in pancreatic neuroendocrine neoplasms
Authors: Schimmack Simon , Yang Yongchao , Felix Klaus , Herbst Markus , Li Yixiong , Schenk Miriam , Bergmann Frank , Hackert Thilo , Strobel Oliver
Published: June 2019

Objective

Elevated pre-operative C-reactive protein (CRP) serum values have been reported to be associated with poor overall survival for patients with pancreatic neuroendocrine neoplasms (pNEN). The aim of this study was to identify mechanisms linking CRP to poor prognosis in pNEN.

Methods

The malignant properties of pNENs were investigated using the human pNEN cell-lines BON1 and QGP1 exposed to CRP or IL-6. Analyses were performed by ELISA, Western blot, flow cytometry and immunocytochemistry as well as invasion and proliferation assays. To compare cytokine profiles and CRP levels, 76 serum samples of pNEN patients were analyzed using Luminex technology. In parallel, the expression of CRP and growth signaling pathway proteins was assessed on cell lines and paraffin-embedded primary pNEN.

Results

In BON1 and QGP1 cells, inflammation (exposure to IL-6) significantly upregulated CRP expression and secretion as well as migratory properties. CRP stimulation of BON1 cells increased IL-6 secretion and invasion. This was accompanied by activation/phosphorylation of the ERK, AKT and/or STAT3 pathways. Although known CRP receptors – CD16, CD32 and CD64 – were not detected on BON1 cells, CRP uptake of pNEN cells was shown after CRP exposure. In patients, increased pre-operative CRP levels (≥5 mg/L) were associated with significantly higher serum levels of IL-6 and G-CSF, as well as with an increased CRP expression and ERK/AKT/STAT3 phosphorylation in pNEN tissue.

Conclusion

The malignant properties of pNEN cells can be stimulated by CRP and IL-6 promoting ERK/AKT/STAT pathways activation as well as invasion, thus linking systemic inflammation and poor prognosis.
The neuropeptide 26RFa in the human gut and pancreas: potential involvement in glucose homeostasis
Authors: Prévost Gaëtan , Picot Marie , Le Solliec Marie-Anne , Arabo Arnaud , Berrahmoune Hind , El Mehdi Mouna , Cherifi Saloua , Benani Alexandre , Nédélec Emmanuelle , Gobet Françoise , Brunel Valéry , Leprince Jérôme , Lefebvre Hervé , Anouar Youssef , Chartrel Nicolas
Published: June 2019

Objective

Recent studies performed in mice revealed that the neuropeptide 26RFa regulates glucose homeostasis by acting as an incretin and by increasing insulin sensitivity. However, in humans, an association between 26RFa and the regulation of glucose homeostasis is poorly documented. In this study, we have thus investigated in detail the distribution of 26RFa and its receptor, GPR103, in the gut and the pancreas, and determined the response of this peptidergic system to an oral glucose challenge in obese patients.

Design and methods

Distribution of 26RFa and GPR103 was examined by immunohistochemistry using gut and pancreas tissue sections. Circulating 26RFa was determined using a specific radioimmunoassay in plasma samples collected during an oral glucose tolerance test.

Results

26RFa and GPR103 are present all along the gut but are more abundant in the stomach and duodenum. In the stomach, the peptide and its receptor are highly expressed in the gastric glands, whereas in the duodenum, ileum and colon they are present in the enterocytes and the goblet cells. In the pancreatic islets, the 26RFa/GPR103 system is mostly present in the β cells. During an oral glucose tolerance test, plasma 26RFa profile is different between obese patients and healthy volunteers, and we found strong positive correlations between 26RFa blood levels and the BMI, and with various parameters of insulin secretion and insulin resistance.

Conclusion

The present data suggest an involvement of the 26RFa/GPR103 peptidergic system in the control of human glucose homeostasis.
Cyproterone acetate or spironolactone in lowering testosterone concentrations for transgender individuals receiving oestradiol therapy
Authors: Angus Lachlan , Leemaqz Shalem , Ooi Olivia , Cundill Pauline , Silberstein Nicholas , Locke Peter , Zajac Jeffrey D , Cheung Ada S
Published: June 2019

Background

Oestradiol with or without an anti-androgen (cyproterone acetate or spironolactone) is commonly prescribed in transfeminine individuals who have not had orchidectomy; however, there is no evidence to guide optimal treatment choice.

Objective

We aimed to compare add-on cyproterone acetate versus spironolactone in lowering endogenous testosterone concentrations in transfeminine individuals.

Design

Retrospective cross-sectional study.

Methods

We analysed 114 transfeminine individuals who had been on oestradiol therapy for >6 months in two gender clinics in Melbourne, Australia. Total testosterone concentrations were compared between three groups; oestradiol alone (n = 21), oestradiol plus cyproterone acetate (n = 21) and oestradiol plus spironolactone (n = 38). Secondary outcomes included serum oestradiol concentration, oestradiol valerate dose, blood pressure, serum potassium, urea and creatinine.

Results

Median age was 27.0 years (22.5–45.1) and median duration of hormone therapy was 1.5 years (0.9–2.6), which was not different between groups. On univariate analysis, the cyproterone group had significantly lower total testosterone concentrations (0.8 nmol/L (0.6–1.20)) compared with the spironolactone group (2.0 nmol/L (0.9–9.4), P = 0.037) and oestradiol alone group (10.5 nmol/L (4.9–17.2), P < 0.001), which remained significant (P = 0.005) after adjustments for oestradiol concentration, dose and age. Serum urea was higher in the spironolactone group compared with the cyproterone group. No differences were observed in total daily oestradiol dose, blood pressure, serum oestradiol, potassium or creatinine.

Conclusions

The cyproterone group achieved serum total testosterone concentrations in the female reference range. As spironolactone may cause feminisation without inhibition of steroidogenesis, it is unclear which anti-androgen is more effective at feminisation. Further prospective studies are required.
Educación para el amor: una necesidad impostergable
Authors: Steele Zúñiga Gerardo A.
Published: June 2019

Este artículo reconoce los esfuerzos hechos en Costa Rica, concernientes a la Educación Sexual. Señala, también, el bajo nivel de logro en este campo, debido a la falta de coordinación entre las instituciones y las personas preocupadas por la Educación Sexual. Y lo más, importante, propone trabajar fuertemente en Educación para el Amor, enfatizando en los principios éticos y filosóficos del Amor, como los fundamentos de la educación de la sexualidad y el sexo.
Sexualidade e autonomia das mulheres no México: o estado laico em questão
Authors: Amuchástegui Ana , Cruz Guadalupe , Aldaz Evelyn , Mejía María Consuelo
Published: June 2019

No México, o entrelaçamento entre política, religião e igualdade de gênero está mais forte do que nunca. Prova disso é que a disponibilização da pílula do dia seguinte pelos serviços federais de Saúde Pública em 2004 e a descriminalização do aborto em 2008, na cidade do México, tiveram como resposta uma campanha massiva para recriminalizar o aborto. Este artigo examina as práticas e os discursos de diversos atores, dos quais três elementos se destacam: a autonomia das mulheres em matéria de sexualidade e de reprodução, que é objeto de um intenso debate público; a reforma do sistema político que obriga a Igreja Católica a se sujeitar às regras da democracia; e a dimensão laica do Estado, que dá lugar a uma luta implacável, reconfigurando as fronteiras políticas e ideológicas em vigor.
Gamification in Management: a systematic review and research directions
Authors: Wanick Vanissa , Bui Hong
Published: June 2019

This paper conducts a contemporary and inclusive review of initial applications of gamification to various management fields, such as finance, corporate governance, risk management, human resource management, etc. It defines and contextualizes gamification within the field of management. This allows to understand the main elements of a general gamified system and its main applications. Via a systematic review of 203 studies addressing management contexts and gamification, it develops a holistic framework for the analysis of gamification in management areas. The study concludes by setting out a list of questions to direct future research in order to increase the impacts of this innovative and strategic research area within businesses and organizations.
Capacity Building on Heat Balance in Electrolysis of Aluminium
Authors: Petersen Sobah Abbas , Oliveira Manuel , Hestetun Kristin , Müller Gunn Iren , Wasbø Stein O. , Koritzinsky Victoria , Wasl Anne Lise
Published: June 2019

Designing games for learning in the workplace is challenging in many ways. Within the process industry, the aluminium production cells are harsh environments that are difficult to instrument and pose domain related challenges, which make effective training difficult to achieve. In particular, cognitive challenges arise due to the slow nature of the physical process that disconnects the operators' actions and causality. The complex chemical process demands a high level of understanding of the parameters and their complex interdependencies. Additional challenges are related to representing an appropriate level of detail and precision of the workplace, including integration with existing tools. This paper describes the digital simulation game designed and developed for the operators in aluminium plants to master the cognitive skills required for Heat Balance in the aluminium production cells. A co-design process was adopted from the initial inception of the idea, development and evaluation. The paper will discuss and present the co-creation artefacts used, namely a paper prototype, hybrid prototype of the game along with the output from iterative development cycles. This work is conducted within the research and innovation project, Accelerated Learning for Technology Transfer, with the Norwegian company Hydro.
Introduction to the special issue on “Gamification of Industrial Systems”
Authors: Garbaya Samir , Lim Theodore
Published: June 2019

This special issue marks important milestones for the Serious Gaming Society (SGS) and its journal the International Journal of Serious Games. First, this special issue is dedicated to the gamification of industrial systems in the context of the widespread adoption of serious games in many fields such as education, medicine, military and industry. The potential for training by increasing motivation for engaged interaction and the adaptation of behavior has been proven. In addition, new emerging needs for gamification are identified in industry where important benefits could be generated by reducing the product cost and time-to-market. Moreover, in the era of industry 4.0, which combines production methods with the state-of-the art information and communication technologies, serious games could have the potential to promote innovation and increase the resources and the production efficiency. However, despite the significant advances that have been made in game-based approaches to non-game contexts, its potential transition into industrial settings have not been realized. This is mainly due to challenging issues such as the lack of methodologies for modeling industrial scenarios in digital contents representing real industrial systems with high fidelity, operational processes must be fully digitalized to ease the integration of gamification concepts, the adaptation of work environment conditions so that the use of gaming on mobile devices can take place without the interruption of industrial activities.
Why Choose Lifeasible As Organic Contaminants Testing Service Provider v1
Authors: Miller Ilsa
Published: June 2019

Lifeasible, a superior in the field of scientific services, is committed to helping customers accelerate researches in the life science field, solve complex problems and challenges in science analysis, promote medical diagnostic development, and improve laboratory efficiency and productivity. With a professional testing team, advanced techniques and excellent service, Lifeasible does a good job in organic contaminants testings.
Scarless introduction of point mutations in mammalian cells with S. pyogenes Cas9 v1
Authors: Tejura Malvika , Floor Stephen
Published: June 2019

This protocol outlines the steps to edit single point mutations into cells in a scarless manner. Uses single-stranded DNA repair templates and the S. pyogenes CRISPR/Cas9 system. Using a short single-stranded DNA repair template is advantageous because it is easy to order synthetically and it cannot be randomly integrated into other double-strand breaks in the cell. Furthermore, this protocol is "scarless" - that is, nothing is changed to the genome other than the desired changes. However, it may be easier to edit genes using a selectable marker, especially when knocking out a gene. This protocol is ideal when trying to make genetic changes with minimal perturbations to the gene locus and surroundings. Note that repair using a single-stranded short repair template (SSTR) uses a different DNA repair pathway than homology-directed repair from long DNA repair templates. Citations: image 1: synthego.com- verify your guide design tool
WATER POLO GAME: A COMPARATIVE STUDY OF MEN'S NATIONAL TEAM BETWEEN INDONESIA AND SINGAPORE IN THE 28TH SEA GAMES 2015
Authors: Mulyania Jajang Dede , Mulyana Boyke , Subarjah Herman
Published: June 2019

Penelitian ini bertujuan untuk mengetahui statistik pertandingan dan aktivitas para pemain dari tim nasional polo air Indonesia dibandingkan terhadap tim nasional polo air Singapura pada Sea Games ke-28 tahun 2015. Data penelitian dikumpulkan dengan menggunakan instrumen yang diadopsi dari studi Escalante dan Platanou yang meneliti mengenai statistik pertandingan dan aktivitas gerak pemain polo air. Hasil penelitian menunjukkan bahwa tim nasional polo air Singapura memiliki jumlah gol lebih banyak dan memiliki jumlah aspek pelanggaran yang lebih rendah dibandingkan dengan tim Indonesia. Selain itu, timnas Indonesia lebih banyak bertahan dan tidak melakukan penyerangan ketika bertemu lawan. Sebaliknya, pada tim Singapura tim pertahanan tidak banyak melakukan kontak fisik ketika bertemu dengan lawan.
PENDIDIKAN NILAI MELALUI DIALEKTIKA ETIK DALAM KONSEP PSIKOANALISIS FREUD (PADA PEMBELAJARAN BAHASA ASING)
Authors: Juami Ratna
Published: June 2019

Penelitian ini mengkaji pendidikan nilai melalui pembelajaran bahasa asing (Bahasa Jerman) pada kelas X di SMA PGII I Bandung sebagai proyek Sekolah Mitra di pulau Jawa. Rekonstruksi nilai dilihat dalam konsep psikoanalisis Freud. Dialektika ethics memperlihatkan signifikansi koheren terhadap konstruksi asumsi peserta didik mengenai nilai dalam proses pembelajaran. Menggunakan pendekatan kualitatif deskriptif yang diperoleh dari wawancara, kuisioner, dan pengamatan partisipan pada kelas X.1, X.3 dan kelas Intensif serta 3 orang alumni SMA PGII I Bandung, penelitian ini melihat dialektika ethics dalam merekonstruksi nilai, dan mengkajinya dalam konsep psikoanalisis Freud. Penelitian ini menemukan bahwa kedisiplinan dan konsistensi guru dalam proses pembelajaran menumbuhkan tanggung jawab peserta didik sebagai manifestasi ethics. Guru berhasil menciptakan nilai etik (norma) kelas, yang secara perlahan dan tanpa disadari peserta didik membentuk sensor moral (superego). Implementasi Pendidikan Nilai melalui pembelajaran bahasa asing terepresentasikan melalui konsep psikoanalisis Freud dalam ranah sensor moral (superego) peserta didik. Kedisiplinan seorang guru mampu merefleksikan tanggung jawab peserta didik yang mengimplementasikan pendidikan nilai dalam proses kegiatan pembelajaran bahasa asing (bahasa Jerman).
PRINCIPLES OF SENSE FORMATION IN THE PHILOSOPHICAL FAIRY TALE "PICTOR'S METAMORPHOSES" BY HERMANN HESSE
Authors:   Taras Shevchenko National University of Kyiv, Kyiv, Ukraine, Megela Ivan
Published: June 2019

Deep analysis of the fairy tale is presented as a periphrasis of mystery of life, the work is traced back to its inception, symbolic value of images, magical and alchemical transformation of physical matter is depicted. The article considers the fundamental hermetic principle "As above, so below; As within, so without" that suggests that human consciousness creates reality, Eastern worldview is combined with the principle of active imagination by C. G. Jung as a way to develop self-understanding through the work with symbols and the use of a dynamic language of allegory and parable. The article examines creative principle of synchronicity performed as a metaphor and as the one that arranges events not only by "non-physical" (non-causal) mechanisms but on the basis of their sense. The meanings of psychoanalytic symbols are identified: the carbuncle crystal, the biblical serpent, the "tree of life", the fairy-tale bird.
CONCEPT INTERPRETATION FIELD AS A CONDUCTOR BETWEEN CONCEPT UNIVERSALITY AND ITS LINGVOCULTURAL SPECIFICITY
Authors: Popova Natalia ,   Taras Shevchenko National University of Kyiv, Kyiv, Ukraine
Published: June 2019

The article is devoted to the study of the mechanisms of universal concepts transformation into linguocultural mental units, a significant place among which belongs to the concept interpretive field which is defined as a series of cognitive characteristics interpreting the concept image and its notional content during its personal or collective consciousness practical comprehension. Changes within the prototype core and the conceptual field, arising due to the expansion of concept interpretation field during its historical development, have been analyzed in the research. Due to the semantic-cognitive and comparative analysis, changes within the interpretive field of the Spanish concepts CABALLERO, BANDERA and MACHO have been determined. It was clarified that the expansion of the interpretation field is accompanied by the modification of concept notional, associative and axiological components under the influence of social, historical, psychological and emotional peculiarities of the Spaniards. These modifications are reflected in the evaluative, conceptual, paremiological, utilitarian and regulatory zones of the concept interpretative field. It is established that the acquisition of the linguistic-cultural specificity by a universal concept occurs during its transition from the philosophical, religious or state-ideological type of consciousness to the everyday-life one, where the concept is rethought by the Spaniards in accordance with their world view and life realities. The boundaries of the concept interpretation field are expanded by its intellectual reflection under the influence of the people’s historical memory and revealed in literary works, music, painting and cinematography.
Cement Quality Control By Using Modern Radiation Methods Of Chemical Analysis In The Process Of Its Production
Authors: Kuchirka Yu. , Volodarsky E. ,   National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”
Published: June 2019

An essential part of modern quality management system in cement production is state-of-the-art radiation measurement technologies based on methods of neutron activation, X-ray fluorescence and X-ray diffraction chemical analysis of substance. The high speed and accuracy of measuring the characteristics of raw materials and finished products can be achieved by their complex application, thereby ensuring an improving in the level of automation of cement production and the quality of cement in general. The main stages of the portlandcement production process by dry method are considered, including the keypoints of quality control of their implementation by applying mentioned radiation methods of chemical analysis of raw materials and completed product. The metrological problems of their practical implementation in continuous cement production are analyzed, in particular problems of uncertainty assessment, static and dynamic calibration and increase of accuracy of measuring systems that implement neutron activation analysis methods. Shown the directions of their improvement by the use of alternative neutron sources, methods of Monte Carlo N-Particle Transport Code for neutron activation analysis physical processes simulation and machine learning for the efficient processing of spectral characteristics of investigated substances.
Direct Observations of Surface‐Wave Eigenfunctions at the Homestake 3D Array
Authors: Meyers Patrick , Bowden Daniel C. , Prestegard Tanner , Tsai Victor C. , Mandic Vuk , Pavlis Gary , Caton Ross
Published: June 2019

Despite the theory for both Rayleigh and Love waves being well accepted and the theoretical predictions accurately matching observations, the direct observation of their quantifiable decay with depth has never been measured in the Earth’s crust. In this work, we present observations of the quantifiable decay with depth of surface‐wave eigenfunctions. This is done by making direct observations of both Rayleigh‐wave and Love‐wave eigenfunction amplitudes over a range of depths using data collected at the 3D Homestake array for a suite of nearby mine blasts. Observations of amplitudes over a range of frequencies from 0.4 to 1.2 Hz are consistent with theoretical eigenfunction predictions. They show a clear exponential decay of amplitudes with increasing depth and a reversal in sign of the radial‐component Rayleigh‐wave eigenfunction at large depths, as predicted for fundamental‐mode Rayleigh waves. Minor discrepancies between the observed eigenfunctions and those predicted using estimates of the local velocity structure suggest that the observed eigenfunctions could be used to improve the velocity model. Our results confirm that both Rayleigh and Love waves have the depth dependence that they have long been assumed to have. This is an important direct validation of a classic theoretical result in geophysics and provides new observational evidence that classical seismological surface‐wave theory can be used to accurately infer properties of Earth structure and earthquake sources.
Apresentação
Authors: Curi Maria Zilda Ferreira , Martins Leda Maria , Walty Ivete Lara Camargos , Ribeiro Gustavo Silveira
Published: June 2019

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A comida paraense em Belém do Grão Pará de Dalcídio Jurandir
Authors: Dalla Bona Fabiano
Published: June 2019

Resumo: Em Belém do Grão Pará, quarto romance do escritor marajoara Dalcídio Jurandir, grande parte da tradicional gastronomia paraense é descrita com especial atenção. Das cozinhas dos barões da borracha às comidas de rua, pode-se assistir a um desfile de iguarias que atiçam o paladar, a visão, o tato e a audição, entre exotismo e tradição.
Palavras-chave: Dalcídio Jurandir; Belém do Grão Pará; Gastronomia Paraense.
Iguarias literárias do Brasil segundo Piers Armstrong
Authors: Orlando José Antônio
Published: June 2019

Entrevista com Piers Armstrong, professor da Universidade da Califórnia e pesquisador da literatura e da cultura do Brasil. Ele visitou a UFMG em novembro de 2018 e proferiu uma conferência sobre Guimarães Rosa e o Modernimo Europeu. Na entrevista, Piers Armstrong apresenta sua trajetória de estudos e pesquisas sobre autores como Guimarães Rosa e Jorge Amado e sobre a recepção do olhar estrangeiro diante das iguarias literárias e dos vários sabores brasileiros.
Imaginação, memórias e sabores em Baú de ossos
Authors: Gabriel Maria Alice Ribeiro , Santos Luciane Alves
Published: June 2019

Resumo: Patrimônio material e imaterial, a alimentação integra os domínios da literatura e da memória, individual e coletiva. Partindo desse princípio, nosso objetivo neste estudo é apresentar a questão da alimentação como tema específico nas memórias do escritor e memorialista brasileiro Pedro da Silva Nava. Nossa proposta é estabelecer uma abordagem multidisciplinar do tema da alimentação na obra Baú de ossos (1972), com base nos estudos de Gilberto Freyre, Luís da Câmara Cascudo e Séverine Mathieu. A análise assinala que o tema se inscreve no quadro geral das reflexões de Nava sobre a importância da imaginação e da memória na composição do texto literário memorialístico.
Palavras-chave: Pedro Nava; memórias; alimentação.
 
Résumé: Patrimoine immatériel et matériel, l’alimentation integre des domaines de la littérature et de la mémoire, individuelle et collective. En partant de ce principe, notre objectif dans cette étude est de présenter la question de l’alimentation comme un thème spécifique dans le mémoires de l’écrivain et mémorialiste brésilien Pedro da Silva Nava. Notre proposition est d’établir une approche pluridisciplinaire de la thématique de l’alimentation dans l’oeuvre Baú de ossos (1972), en s’appuyant sur les écrits de Gilberto Freyre, Luís da Câmara Cascudo et Séverine Mathieu. L’analyse signale que le thème s’inscrit dans le cadre général des réflexions de Pedro Nava sur l’importance du rôle de l’imagination et de la mémoire pour la composition du texte littéraire mémorialistique.
Mots-clés: Pedro Nava; mémoires; alimentation.
Análise Fitoquímica Preliminar de Extratos Foliares de Orchidaceas (Cattleya walkeriana Gardner), (Oncidium cebolleta Sw.), (Encyclia linearifolioides Kraenzl.) e (Polystachya concreta (Jacq.) Garay & H. R. Sweet)
Authors: Menezes Filho Antonio Carlos Pereira de , Castro Carlos Frederico de Souza
Published: June 2019

As orquídeas são espécies da flora brasileira que merecem atenção quanto ao uso fitoterápico. Este estudo objetivou avaliar por meio de análises fitoquímicas preliminares, os principais grupos químicos que compõem os extratos etanólicos foliares de C. walkeriana, O. cebolleta, E. linearifolioides e P. concreta encontradas no bioma Cerrado. Foram preparados extratos etanólicos foliares e avaliados para os testes de identificação para compostos, ácidos orgânicos, açúcares redutores e não redutores, alcaloides, flavonoides, saponinas espumídicas, cumarinas, glicosídeos cardíacos, fenólicos simples, taninos, polissacarídeos, purinas, catequinas, derivados de quininas, depsídeos e depsidonas, compostos antraquinônicos e duplas olefínicas. Os resultados foram positivos para ácidos orgânicos, açúcares redutores e não redutores, alcaloides, flavonoides, saponinas, cumarinas, glicosídeos cardíacos apenas na reação de Raymond-Marthoud, fenólicos, taninos condensados, depsídeos e depsidonas e duplas olefínicas. Este trabalho apresenta dados inéditos para a composição fitoquímica preliminar das espécies de Orchidaceae avaliadas. Palavras-chave: Prospecção Fitoquímica. Orquídeas. Fenólicos. AbstractOrchids are species of Brazilian flora that deserve attention regarding phytotherapeutic use. This study aimed to evaluate through  preliminary phytochemical analyzes the main chemical groups that compose the foliar ethanolic extracts of C. walkeriana, O. cebolleta, E. linearifolioides and P. concreta found in the Cerrado biome. Foliar ethanolic extracts were prepared for the identification tests for compounds, organic acids, reducing and non-reducing sugars, alkaloids, flavonoids, foamy saponins, coumarins, cardiac glycosides, simple phenolics, tannins, polysaccharides, purines, catechins, depsides and depsidones, anthraquinone compounds and olefinic doublets. The results were positive for organic acids, reducing and non-reducing sugars, alkaloids, flavonoids, saponins, coumarins, cardiac glycosides only in the Raymond-Marthoud reaction, phenolics, condensed tannins, depsides and depsidones and olefinic doubles. This work presents unpublished data for the preliminary phytochemical composition of the Orchidaceae species evaluated. Keywords: Phytochemical Prospecting. Orchids. Phenolics.
Recursos Pesqueiros de Mariscagem: uma Revisão da Literatura Sobre Iphigenia brasiliensis
Authors: Ferreira Maíra Silva Navarro , Duarte Michelle Rezende , Silva Edson Pereira da
Published: June 2019

O bivalve estuarino Iphigenia brasiliensis (Lamarck, 1818) é um recurso de mariscagem amplamente explorado nas regiões Norte e Nordeste do Brasil. Embora a espécie apresente relevância socioeconômica para muitas populações tradicionais, poucas informações a seu respeito estão disponíveis na literatura. Este trabalho teve como objetivo recuperar estudos nos quais I. brasiliensis fosse espécie modelo ou um dado direto da pesquisa. Para tanto, foi realizada uma revisão da literatura utilizando-se de quatro bases de dados digitais. Foram recuperadas 132 publicações, as quais foram caracterizadas por uma análise de conteúdo, bem como tiveram seus dados compilados para uma descrição estatística. Das publicações recuperadas, apenas 17 corresponderam a pesquisas diretamente relacionadas à espécie, ou seja, aquelas em que I. brasiliensis foi o objeto de estudo. A maior parte dos trabalhos levantados nesta revisão referiu-se a espécie, principalmente, como uma das capturas incidentais mais abundantes durante a catação de bivalves comercialmente mais rentáveis. Palavras-chave: Bivalve Estuarino. Catação de Moluscos. Captura Incidental. AbstractIphigenia brasliensis (Lamarck, 1818) is an estuarine bivalve used as commercial and subsistence resource by the traditional communities from North and Northeast of Brazil. Although its socioeconomic relevance, the species is poorly studied and information on it is very rare in the academic literature. The main aim of this review was to recover all available information on I. brasiliensis that was scattered among many different sources. For doing that a search was carried out using four digital databases. 132 publications were recovered and characterized by a content analysis, as well as had their data compiled for a statistical description. Of the recovered publications, only 17 corresponded to research directly related to the species, that is, to those in which I. brasiliensis was the object of study. Most of the literature recovered in this review referred to the species only as one of the most abundant bycatch during the exploitation of other commercially profitable bivalves. Keywords: Estuarine Bivalve. Mollusk Gathering. Bycatch.