bluuebunny/crossref_metadata_2025 huggingface dataset:
222-Nanometer Far-UVC Exposure Results in DNA Damage and Transcriptional Changes to Mammalian Cells
Authors: Ong Qunxiang , Wee Winson , Dela Cruz Joshua , Teo Jin Wah Ronnie , Han Weiping
Published: August 2022

Ultraviolet (UV) germicidal tools have recently gained attention as a disinfection strategy against the COVID-19 pandemic, but the safety profile arising from their exposure has been controversial and impeded larger-scale implementation. We compare the emerging 222-nanometer far UVC and 277-nanometer UVC LED disinfection modules with the traditional UVC mercury lamp emitting at 254 nm to understand their effects on human retinal cell line ARPE-19 and HEK-A keratinocytes. Cells illuminated with 222-nanometer far UVC survived, while those treated with 254-nanometer and 277-nanometer wavelengths underwent apoptosis via the JNK/ATF2 pathway. However, cells exposed to 222-nanometer far UVC presented the highest degree of DNA damage as evidenced by yH2AX staining. Globally, these cells displayed transcriptional changes in cell-cycle and senescence pathways. Thus, the introduction of 222-nanometer far UVC lamps for disinfection purposes should be carefully considered and designed with the inherent dangers involved.
Concomitant Inhibition of IRE1α/XBP1 Axis of UPR and PARP: A Promising Therapeutic Approach against c-Myc and Gammaherpesvirus-Driven B-Cell Lymphomas
Authors: Benedetti Rossella , Arena Andrea , Romeo Maria Anele , Gilardini Montani Maria Saveria , Gonnella Roberta , Santarelli Roberta , Trivedi Pankaj , Cirone Mara
Published: August 2022

It is emerging that targeting the adaptive functions of Unfolded Protein Response (UPR) may represent a promising anti-cancer therapeutic approach. This is particularly relevant for B-cell lymphomas, characterized by a high level of constitutive stress due to high c-Myc expression. In this study, we found that IRE1α/XBP1 axis inhibition exerted a stronger cytotoxic effect compared to the inhibition of the other two UPR sensors, namely PERK and ATF6, in Burkitt lymphoma (BL) cells, in correlation with c-Myc downregulation. Interestingly, such an effect was more evident in Epstein-Barr virus (EBV)-negative BL cells or those cells expressing type I latency compared to type III latency BL cells. The other interesting finding of this study was that the inhibition of IRE1α/XBP1 downregulated BRCA-1 and RAD51 and potentiated the cytotoxicity of PARP inhibitor AZD2661 against BL cells and also against Primary Effusion Lymphoma (PEL), another aggressive B-cell lymphoma driven by c-Myc and associated with gammaherpesvirus infection. These results suggest that combining the inhibition of UPR sensors, particularly IRE1α/XBP1 axis, and molecules involved in DDR, such as PARP, could offer a new therapeutic opportunity for treating aggressive B-cell lymphomas such as BL and PEL.
Functional Crosstalk between PCSK9 Internalization and Pro-Inflammatory Activation in Human Macrophages: Role of Reactive Oxygen Species Release
Authors: Jaén Rafael I. , Povo-Retana Adrián , Rosales-Mendoza César , Capillas-Herrero Patricia , Sánchez-García Sergio , Martín-Sanz Paloma , Mojena Marina , Prieto Patricia , Boscá Lisardo
Published: August 2022

Atherosclerosis is a cardiovascular disease caused mainly by dyslipidemia and is characterized by the formation of an atheroma plaque and chronic inflammation. Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a protease that induces the degradation of the LDL receptor (LDLR), which contributes to increased levels of LDL cholesterol and the progress of atherosclerosis. Given that macrophages are relevant components of the lipidic and inflammatory environment of atherosclerosis, we studied the effects of PCSK9 treatment on human macrophages. Our data show that human macrophages do not express PCSK9 but rapidly incorporate the circulating protein through the LDLR and also activate the pro-inflammatory TLR4 pathway. Both LDLR and TLR4 are internalized after incubation of macrophages with exogenous PCSK9. PCSK9 uptake increases the production of reactive oxygen species and reduces the expression of genes involved in lipid metabolism and cholesterol efflux, while enhancing the production of pro-inflammatory cytokines through a TLR4-dependent mechanism. Under these conditions, the viability of macrophages is compromised, leading to increased cell death. These results provide novel insights into the role of PCSK9 in the crosstalk of lipids and cholesterol metabolism through the LDLR and on the pro-inflammatory activation of macrophages through TLR4 signaling. These pathways are relevant in the outcome of atherosclerosis and highlight the relevance of PCSK9 as a therapeutic target for the treatment of cardiovascular diseases.
Carrageenan-Based Compounds as Wound Healing Materials
Authors: Neamtu Bogdan , Barbu Andreea , Negrea Mihai Octavian , Berghea-Neamțu Cristian Ștefan , Popescu Dragoș , Zăhan Marius , Mireșan Vioara
Published: August 2022

The following review is focused on carrageenan, a heteroglycan-based substance that is a very significant wound healing biomaterial. Every biomaterial has advantages and weaknesses of its own, but these drawbacks are typically outweighed by combining the material in various ways with other substances. Carrageenans’ key benefits include their water solubility, which enables them to keep the wound and periwound damp and absorb the wound exudate. They have low cytotoxicity, antimicrobial and antioxidant qualities, do not stick to the wound bed, and hence do not cause pain when removed from the wounded region. When combined with other materials, they can aid in hemostasis. This review emphasizes the advantages of using carrageenan for wound healing, including the use of several mixes that improve its properties.
Efficacy of Spinetoram for the Control of Bean Weevil, Acanthoscelides obtectus (Say.) (Coleoptera: Chrysomelidae) on Different Surfaces
Authors: Sağlam Özgür , Çelik Ahmet , Işıkber Ali Arda , Bozkurt Hüseyin , Sakka Maria K. , Athanassiou Christos G.
Published: August 2022

In this study, the contact toxicity of spinetoram on three different surfaces, concrete, ceramic floor tile and laminate flooring, against Acanthocelides obtectus (Say.) (Coleoptera: Chrysomelidae) was evaluated in laboratory bioassays. Different concentrations were evaluated ranging from 0.0025 to 0.05 mg AI/cm2, against adults of A. obtectus. Adult mortality was measured after 1-, 3-, 5- and 7-day exposure. After 1-day exposure, the mortality was low on all surfaces, ranging from 0 to 27.2%. After 5- and 7-day exposure, spinetoram at concentrations of 0.01 mg/cm2 and above achieved 100% or close mortality on concrete and laminate flooring surface, whereas low concentrations (0.0025, 0.005 and 0.0075 mg AI/cm2) resulted in significantly lower mortality levels, ranging from 1.6 to 30.8%, than high concentrations. In the case of ceramic floor tile surface, spinetoram treatments at all tested concentrations did not result in 100% mortality. Significant differences were recorded among the surfaces, depending on concentrations and exposure intervals. After 3-, 5- and 7-day exposure, mortality levels on ceramic floor tile surface were generally higher at low concentrations than those on the concrete and laminate flooring surfaces, whereas those on concrete and laminate flooring surfaces were significantly higher at high concentrations than ceramic floor tile surface. These results indicate that spinetoram at 0.025 and 0.05 mg AI/cm2 achieve satisfactory control at relatively short exposures on common types of surfaces and thus can be used as an effective insecticide against A. obtectus.
Effects of Adult Feeding Treatments on Longevity, Fecundity, Flight Ability, and Energy Metabolism Enzymes of Grapholita molesta Moths
Authors: Su Sha , Zhang Xiaohe , Jian Chengzhi , Huang Baojian , Peng Xiong , Vreysen Marc J. B. , Chen Maohua
Published: August 2022

The oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae) is one of the most severe fruit tree pests in China, causing huge economic losses to fruit production. So far, there are few detailed reports on the rearing protocols of G. molesta. In this study, we compared the longevity, 10-d fecundity, flight ability, and the activity of major energy metabolism enzymes in the flight muscles of G. molesta under three feeding regimes (supplement with sterile water, supplement with 10% honey solution and starvation) of the adult moths. The results showed that the longevity, 10-d fecundity, and flight parameters (cumulative flight distance and time, maximum flight distance and duration, and the average flight speed) of adult moths when supplemented with sterile water or honey solution were significantly higher than those of moths that were starved. There were no significant differences in the 10-d fecundity, flight parameters, and the activity of major energy metabolism enzymes of flight muscles between moths that were supplemented with sterile water or 10% honey solution. The flight muscles of G. molesta mainly used carbohydrates as an energy source when sterile water and honey solution were supplemented, and the moth mainly used lipids as an energy source under starvation. Considering the cost and potential for diet contamination during mass-rearing, supplying sterile water is considered a cost effective option for food substitution of adult G. molesta.
Angiotensin II Receptor Blocker Irbesartan Enhanced SIRT1 longevity Signaling Replaces the Mitochondrial Biogenetic Survival Pathway to Attenuate Hypertension-Induced Heart Apoptosis
Authors: Pai Pei-Ying , Wong James K. S. , Cui Zhen-Yang , Lin Yi-Yuan , Lee Shin-Da
Published: August 2022

Background: The present study investigated whether angiotensin II type 1 receptor blocker irbesartan (ARB) and partial agonist of PPAR-γ prevents heart apoptosis by suppressing cardiac Fas/FasL-mediated to mitochondria-mediated apoptosis in the hearts of hypertensive rat model. Methods: Cardiac function using echocardiography, H&E staining, TUNEL assay, and Western blotting were measured in the excised hearts from three groups, i.e., an untreated hypertensive group (SHR), an ARB-treated hypertensive group (50 mg/kg/day, S.C., SHR-ARB), and untreated normotensive Wistar-Kyoto rats (WKY). Results: Fas Ligand, Fas death receptors, FADD, active caspase-8, active caspase-3 (Fas/FasL-mediated apoptotic pathway), as well as Bax, cytochrome c, active caspase-9 and -3 (mitochondria-mediated apoptotic pathway), IGF-II, and p-JNK were decreased in SHR-ARB group when compared with the SHR group. SIRT1, PGC-1α, Bcl2, and Bcl-xL (SIRT1/PGC-1α pro-survival pathway) were increased in the SHR-ARB group when compared with the SHR group. Conclusions: Our findings suggested that the ARB might prevent cardiac Fas/FasL-mediated to mitochondria-mediated apoptosis pathway in the hypertensive model associated with IGF-II, p-JNK deactivation, and SIRT1/PGC-1α pro-survival pathway upregulation. ARB prevents hypertension-enhanced cardiac apoptosis via enhancing SIRT1 longevity signaling and enhances the mitochondrial biogenetic survival pathway.
Metronomic Chemotherapy for Metastatic Breast Cancer Treatment: Clinical and Preclinical Data between Lights and Shadows
Authors: Cazzaniga Marina Elena , Capici Serena , Cordani Nicoletta , Cogliati Viola , Pepe Francesca Fulvia , Riva Francesca , Cerrito Maria Grazia
Published: August 2022

Metronomic chemotherapy (mCHT), defined as continuous administration of low-dose chemotherapeutic agents with no or short regular treatment-free intervals, was first introduced to the clinic in international guidelines in 2017, and, since then, has become one of the available strategies for the treatment of advanced breast cancer (ABC). Despite recent successes, many unsolved practical and theoretical issues remain to be addressed. The present review aims to identify the “lights and shadows” of mCHT in preclinical and clinical settings. In the preclinical setting, several findings indicate that one of the most noticeable effects of mCHT is on the tumor microenvironment, which, over the last twenty years, has been demonstrated to be pivotal in supporting tumor cell survival and proliferation. On the other hand, the direct effects on tumor cells have been less well-defined. In addition, critical items to be addressed are the lack of definition of an optimal biological dose (OBD), the method of administration of metronomic schedules, and the recognition and validation of predictive biomarkers. In the clinical context—where mCHT has mainly been used in a metastatic setting—low toxicity is the most well-recognised light of mCHT, whereas the type of study design, the absence of randomised trials and uncertainty in terms of doses and drugs remain among the shadows. In conclusion, growing evidence indicates that mCHT is a suitable treatment option for selected metastatic breast cancer (MBC) patients. Moreover, given its multimodal mechanisms of action, its addition to immunological and targeted therapies might represent a promising new approach to the treatment of MBC. More preclinical data are needed in this regard, which can only be obtained through support for translational research as the key link between basic science and patient care.
Frailty Assessed with FRAIL Scale and G8 Questionnaire Predicts Severe Postoperative Complications in Patients Receiving Major Head and Neck Surgery
Authors: Kunz Viktor , Wichmann Gunnar , Wald Theresa , Pirlich Markus , Zebralla Veit , Dietz Andreas , Wiegand Susanne
Published: August 2022

Introduction: Frailty represents a complex geriatric syndrome associated with elevated rates of postoperative complications as shown for several malignant entities, including head and neck cancer. A specific screening instrument to assess frailty in head and neck patients does not exist. Both the FRAIL Scale and the G8 questionnaire are well-established and easy to use as screening tools. The present study’s aim was to assess the potential of frailty screening to predict postoperative complications in head and neck patients prior to surgery. Patients and methods: We recorded demographic data, pre-existing medical conditions and clinical characteristics in a prospective cohort of 104 head and neck cancer patients undergoing major head and neck surgery and assessed frailty prospectively on the day of admission utilizing the G8 questionnaire and the FRAIL Scale. We analyzed the link between occurrence of postoperative complications up to the twenty-first postoperative day and age, frailty and other covariates using χ2 tests and receiver operating characteristic (ROC) curves. Results: There was no significant correlation between patients’ pre-existing medical conditions and postoperative complications. Whereas chronological age alone did not predict the occurrence of postoperative complications, frailty posed the highest risk for complications. Frailty according to either the G8 questionnaire or the FRAIL Scale predicted occurrence of complications with an area under the curve (AUC) of 0.64 (p = 0.018) and 0.62 (p = 0.039) and severe complications with an AUC of 0.72 (p = 0.014) and 0.69 (p=0.031), respectively. Neither frailty score correlated with age or with each other. Conclusion: Prospective screening using the FRAIL Scale or the G8 questionnaire reliably detected frailty in our sample group. Frailty is linked to increased risk of postoperative complications. The correct prediction of severe postoperative complications as shown identifies vulnerable cases and triggers awareness of potential complications. Anticipating risk allows for a more comprehensive view of the patient and triggers decision making towards risk adjustment, and therefore a selective view of alternative treatment modalities.
Custom Bone Regeneration (CBR): An Alternative Method of Bone Augmentation—A Case Series Study
Authors: De Santis Daniele , Umberto Luciano , Dario Donadello , Paolo Faccioni , Zarantonello Morris , Alberti Cristian , Verlato Giuseppe , Gelpi Federico
Published: August 2022

We performed this clinical study in order to evaluate the reliability of the Guided Bone Regeneration (GBR) surgical technique through the use of customized CAD CAM titanium meshes (Yxoss CBR® Reoss) in order to show an alternative method of bone augmentation. Materials and methods: Nine patients presenting 10 bone defects were referred to solve oral dysfunction due to edentulous atrophic ridges. Guided bone regeneration was performed with titanium meshes combined with autogenous bone grafting and heterologous bovine bone mineral grafting, and exclusively a “poncho technique” soft tissue approach for all the cases. After a mean 9 months of graft healing (range 6–12 months), titanium meshes were removed, and implant surgery was subsequently performed. The results we obtained were positive in terms of volumetric increases in height, length and thickness of the atrophic ridges without biological complications detectable before implant surgery. Results: Out of nine, one site met titanium mesh exposure: however, in all 10 sites a three-dimensional volumetric bone implementation was obtained. The statistical results were estimated by uploading and superimposing cbct scans before and after CBR surgery for each patient, so it was possible evaluate the maximum linear vertical and horizontal bone gain through dedicated Cad Cam software (Exocad GmbH®). The average horizontal gain was 6.37 ± 2.17 mm (range 2.78–9.12 mm) and vertical gain was 5.95 ± 2.06 mm (range 2.68–9.02 mm). A total of 18 implants were placed into the grafted sites with a 100% survival rate (clearly they are relative percentages to be compared to the short time elapsed). Conclusions: The results we obtained in this study suggest that this CBR procedure (Yxoss® by Reoss) is reliable and safe for bone regeneration to allow implant-prosthetic restoration in horizontal, vertical and combined bone defects. The soft tissue management is diriment: all the cases were managed with a “poncho” flap approach to decrease exposure complication.
Diet and Stone Disease in 2022
Authors: Dai Jessica C. , Pearle Margaret S.
Published: August 2022

Diet plays a central role in the development and prevention of nephrolithiasis. Although pharmacologic treatment may be required for some patients who are resistant to dietary measures alone, dietary modification may be sufficient to modulate stone risk for many patients. While there is no single specialized diet for stone prevention, several dietary principles and recommendations for stone prevention are supported by practice guidelines, including adequate fluid intake, modest calcium intake, low dietary sodium, and limited animal protein. In this review, we summarized the evidence supporting these dietary recommendations and reviewed the current literature regarding specific dietary components and comprehensive diets for stone prevention.
Ferric Carboxymaltose and Erythropoiesis-Stimulating Agent Treatment Reduces the Rate of Blood Transfusion in Refractory Anemia
Authors: Gidaro Antonio , Delitala Alessandro Palmerio , Berzuini Alessandra , Soloski Mark J. , Manca Pietro , Castro Dante , Salvi Emanuele , Manetti Roberto , Lambertenghi Deliliers Giorgio , Castelli Roberto
Published: August 2022

Background: Erythropoiesis-stimulating agents (ESAs) are used to treat refractory anemia (RA). Guidelines suggest iron supplementation for unresponsive patients, regardless of iron deficiency. The primary aim of this study was to evaluate the effect of iron supplementation with ferric carboxymaltose (FCM) on the reduction of red blood cell transfusion (RBCT) rate in transfusion-dependent RA patients. Methods: This was a prospective quasi-randomized study, wherein patients were randomly assigned into three groups: (A) ESAs alone, (B) ferric gluconate (FG) and ESAs, and (C) FCM and ESAs. Hemoglobin and ferritin levels, as well as the number of RBCTs at 4 and 28 weeks were compared. Economic evaluation was also performed. Results: A total of 113 RA patients were enrolled. In total, 43 were treated with intravenous FG and ESAs, 38 with FCM and ESAs, and 32 with ESAs alone. At both follow-ups, erythropoietic response was increased in those receiving iron as compared with those with ESAs alone (p = 0.001), regardless of the type of iron. At one month, ferritin levels were higher in the FCM and ESA groups (p = 0.001). RBCTs were lower in both iron groups. The less costly treatment strategy was FCM, followed by FG, and lastly ESAs. Conclusions: Addition of iron to ESAs in RA reduced RBCT requirement and improved hemoglobin values.
Controversies and Clinical Applications of Non-Invasive Transspinal Magnetic Stimulation: A Critical Review and Exploratory Trial in Hereditary Spastic Paraplegia
Authors: Carra Rafael Bernhart , Silva Guilherme Diogo , Paraguay Isabela Bruzzi Bezerra , Diniz de Lima Fabricio , Menezes Janaina Reis , Pineda Aruane Mello , Nunes Glaucia Aline , Simões Juliana da Silva , França Marcondes Cavalcante , Cury Rubens Gisbert
Published: August 2022

Magnetic stimulation is a safe, non-invasive diagnostic tool and promising treatment strategy for neurological and psychiatric disorders. Although most studies address transcranial magnetic stimulation, transspinal magnetic stimulation (TsMS) has received recent attention since trials involving invasive spinal cord stimulation showed encouraging results for pain, spasticity, and Parkinson’s disease. While the effects of TsMS on spinal roots is well understood, its mechanism of action on the spinal cord is still controversial. Despite unclear mechanisms of action, clinical benefits of TsMS have been reported, including improvements in scales of spasticity, hyperreflexia, and bladder and bowel symptoms, and even supraspinal gait disorders such as freezing and camptocormia. In the present study, a critical review on the application of TsMS in neurology was conducted, along with an exploratory trial involving TsMS in three patients with hereditary spastic paraplegia. The goal was to understand the mechanism of action of TsMS through H-reflex measurement at the unstimulated lumbosacral level. Although limited by studies with a small sample size and a low to moderate effect size, TsMS is safe and tolerable and presents consistent clinical and neurophysiological benefits that support its use in clinical practice.
Circulating Osteogenic Progenitor Cells Enhanced with Teriparatide or Denosumab Treatment
Authors: Giner Mercè , Vázquez-Gámez María Angeles , Miranda María José , Bocio-Nuñez Jesús , Olmo-Montes Francisco Jesús , Rico Miguel Angel , Colmenero Miguel Angel , Montoya-García María-José
Published: August 2022

Circulating osteogenic precursor (COP) cells are peripheral blood cells with a capacity for osteogenesis. The objective of our study was to ascertain the percentage of COPs as an early biomarker of osteoporosis and the effect of these cells in response to Denosumab (DmAb) (anti-resorptive) or to Teriparatide (TPDP) (anabolic) as very effective drugs in the treatment of the illness. A first study was conducted on healthy volunteers, with three age ranges, to determine the percentage of COPs and relate it to their anthropometric and biochemical characteristics, followed by a second longitudinal study on patients with osteoporosis, whereby one group of patients was treated with TPTD and another with DmAb. All were analyzed by cytometry for COP percentage in blood, bone turnover markers, and bone mass. Our findings show that COPs are influenced by age and become more prolific in the stages of growth and skeletal maturation. A higher percentage of COPs is found in osteoporotic disease, which could constitute a predictive marker thereof. We also show how treatment with TPTD or DmAb mobilizes circulating osteogenic precursors in the blood. Significant increases in % COPs were observed after 12 months of treatment with Dmb (21.9%) and TPTD (17%). These results can be related to an increase in osteogenesis and, consequently, a better and more efficient repair of bone tissue.
Three-Dimensional Bioprinting for Cartilage Tissue Engineering: Insights into Naturally-Derived Bioinks from Land and Marine Sources
Authors: Szychlinska Marta Anna , Bucchieri Fabio , Fucarino Alberto , Ronca Alfredo , D’Amora Ugo
Published: August 2022

In regenerative medicine and tissue engineering, the possibility to: (I) customize the shape and size of scaffolds, (II) develop highly mimicked tissues with a precise digital control, (III) manufacture complex structures and (IV) reduce the wastes related to the production process, are the main advantages of additive manufacturing technologies such as three-dimensional (3D) bioprinting. Specifically, this technique, which uses suitable hydrogel-based bioinks, enriched with cells and/or growth factors, has received significant consideration, especially in cartilage tissue engineering (CTE). In this field of interest, it may allow mimicking the complex native zonal hyaline cartilage organization by further enhancing its biological cues. However, there are still some limitations that need to be overcome before 3D bioprinting may be globally used for scaffolds’ development and their clinical translation. One of them is represented by the poor availability of appropriate, biocompatible and eco-friendly biomaterials, which should present a series of specific requirements to be used and transformed into a proper bioink for CTE. In this scenario, considering that, nowadays, the environmental decline is of the highest concerns worldwide, exploring naturally-derived hydrogels has attracted outstanding attention throughout the scientific community. For this reason, a comprehensive review of the naturally-derived hydrogels, commonly employed as bioinks in CTE, was carried out. In particular, the current state of art regarding eco-friendly and natural bioinks’ development for CTE was explored. Overall, this paper gives an overview of 3D bioprinting for CTE to guide future research towards the development of more reliable, customized, eco-friendly and innovative strategies for this field of interest.
Laboratory Fracture Resilience of Hybrid Abutments Used in Oral Rehabilitation: A Systematic Review
Authors: Favasuli Luca , Mascarenhas Paulo S. , Mauricio Paulo
Published: August 2022

When implants are required in prosthodontics treatment, one of the most important decisions is the choice of the final crown and the type of connection to the implant through the abutment. Hybrid abutments are becoming a primary choice. They are projected and produced with materials whose properties guarantee the required mechanical features (including resistance) and take advantage of the hybrid abutment crown retention between screw and cement. However, a review of the mechanical resistance of the different abutment types and associated materials is still lacking. This review aimed to study the in vitro mechanical efficiency of the hybrid abutments used in oral rehabilitation. Methods: A systematic review was conducted using the PubMed, B-on, and Google Scholar databases according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Results: 75 articles were identified from all databases, and 33 were selected after abstract screening. Thus, 21 studies were included in the review after full-text reading. Among the materials used for crowns, lithium disilicate was, aesthetically, the primary choice for its aesthetic and moderate strength. On the other hand, zirconia showed the best fracture resistance. Regarding the different kinds of abutments, there is still some lack of knowledge about the best design. Conclusions: Within the limitations of this systematic review, we can conclude that hybrid pillars are an excellent choice for oral rehabilitation through implants, showing improved resistance when including materials such as zirconia and lithium disilicate.
Impacts of Warming and Acidification on Coral Calcification Linked to Photosymbiont Loss and Deregulation of Calcifying Fluid pH
Authors: Cameron Louise P. , Reymond Claire E. , Bijma Jelle , Büscher Janina V. , De Beer Dirk , Guillermic Maxence , Eagle Robert A. , Gunnell John , Müller-Lundin Fiona , Schmidt-Grieb Gertraud M. , Westfield Isaac , Westphal Hildegard , Ries Justin B.
Published: August 2022

Corals are globally important calcifiers that exhibit complex responses to anthropogenic warming and acidification. Although coral calcification is supported by high seawater pH, photosynthesis by the algal symbionts of zooxanthellate corals can be promoted by elevated pCO2. To investigate the mechanisms underlying corals’ complex responses to global change, three species of tropical zooxanthellate corals (Stylophora pistillata, Pocillopora damicornis, and Seriatopora hystrix) and one species of asymbiotic cold-water coral (Desmophyllum pertusum, syn. Lophelia pertusa) were cultured under a range of ocean acidification and warming scenarios. Under control temperatures, all tropical species exhibited increased calcification rates in response to increasing pCO2. However, the tropical species’ response to increasing pCO2 flattened when they lost symbionts (i.e., bleached) under the high-temperature treatments—suggesting that the loss of symbionts neutralized the benefit of increased pCO2 on calcification rate. Notably, the cold-water species that lacks symbionts exhibited a negative calcification response to increasing pCO2, although this negative response was partially ameliorated under elevated temperature. All four species elevated their calcifying fluid pH relative to seawater pH under all pCO2 treatments, and the magnitude of this offset (Δ[H+]) increased with increasing pCO2. Furthermore, calcifying fluid pH decreased along with symbiont abundance under thermal stress for the one species in which calcifying fluid pH was measured under both temperature treatments. This observation suggests a mechanistic link between photosymbiont loss (‘bleaching’) and impairment of zooxanthellate corals’ ability to elevate calcifying fluid pH in support of calcification under heat stress. This study supports the assertion that thermally induced loss of photosymbionts impairs tropical zooxanthellate corals’ ability to cope with CO2-induced ocean acidification.
A Multidisciplinary Approach for A Better Knowledge of the Benthic Habitat and Community Distribution in the Central and Western English Channel
Authors: Dauvin Jean-Claude , Pezy Jean-Philippe , Poizot Emmanuel , Lozach Sophie , Trentesaux Alain
Published: August 2022

About 80% of the seabed of the English Channel (EC) is covered by coarse sediment, from coarse sand to pebbles. Quantitative data on the benthic macrofauna in these types of sediment remains are rare due to the difficulty of using grab corers in such hard substrates. The deepest central part of the EC (45–101 m depth) was prospected during two VIDEOCHARM surveys in June 2010 and June 2011 to increase knowledge of such sublittoral coarse sediment benthic habitats. Sampling focussed on a longitudinal transect in the deepest part of the EC (13 boxes), extending from the western approach to the Greenwich meridian. Both indirect (side scan sonar, Remote Operated Vehicule) and direct (grab sampling with benthos determination, and grain-size analyses) approaches were used and combined, permitting description of the benthic habitats and communities using seven methods. Five benthic EUNIS habitats (European Nature Information System) were reported: MC3215, MD3211, MC4, MC3212 and MC4215, of which two extended main habitats (MC3211 and M23212) corresponded to an eastern/western gradient from sandy gravel to sandy gravel and pebbles sediment. Three other spatially discrete habitats were associated with poor coarse sand and gravel habitats as well as sandy gravel and pebbles with the presence of the brittle star Ophiothrix fragilis. Taxonomic richness of both extended habitats was on the same order of magnitude as the coarse sand habitat reported elsewhere in the EC, whilst the abundances were among the lowest in deeper areas with low nutrient input and low primary production. The epifauna appeared relatively homogenous in this type of sediment at the scale of the sampling area and was not determined to assign a EUNIS habitat/class. ROV footage illustrated the presence of large epifauna and provided valuable information to ground truth in other sampling methods such as side scan sonar mosaic. Grab photos showing surface sediment was relevant to determine the sediment type, whilst granulometric analyses gave additional information on fine particles content (typically very low).
Isolation and Identification of Luminescent Bacteria in Deep Sea Marine Organisms from Sicilian Waters (Mediterranean Sea)
Authors: Calogero Rosario , Rizzo Carmen , Arcadi Erika , Stipa Maria Giulia , Consoli Pierpaolo , Romeo Teresa , Battaglia Pietro
Published: August 2022

Luminescent bacteria are a fascinating component of marine microbial communities, often related to the light emissions in deep sea marine organisms. They are mainly affiliated with specific phylogenetic groups, such as Photobacterium, Vibrio, and Photorhabdus, and are sometimes involved in symbiotic relationships. However, the luminescence of some marine organisms remains a poorly understood process, and it is not always certain whether their luminescence is attributable to associated luminescent bacteria. In this study, for the first time, luminescent bacteria were isolated from two deep sea organisms, namely, the cephalopod Neorossia caroli and the teleost Chlorophthalmus agassizi. The isolation was carried out on glycerol-supplemented medium, and the search for the luxAB gene was performed on all isolates as a complementary tool to the culture-dependent techniques to detect bioluminescence by molecular approach. The optimum of salinity, temperature, and pH was evaluated by physiological tests for all isolates. The production of extracellular polymeric substances was also preliminarily screened. A total of 24 luminescent isolates were obtained, with an abundance from C. agassizi specimens. All the isolates were taxonomically characterized and were related to different species of Photobacterium, with the exception of Vibrio sp. CLD11 that was from C. agassizi. The luxAB gene was detected in about the 90% of the analysed strains.
Prediction of Emission Characteristics of Generator Engine with Selective Catalytic Reduction Using Artificial Intelligence
Authors: Park Min-Ho , Lee Chang-Min , Nyongesa Antony John , Jang Hee-Joo , Choi Jae-Hyuk , Hur Jae-Jung , Lee Won-Ju
Published: August 2022

Eco-friendliness is an important global issue, and the maritime field is no exception. Predicting the composition of exhaust gases emitted by ship engines will be of consequence in this respect. Therefore, in this study, exhaust gas data were collected from the generator engine of a real ship along with engine-related data to predict emission characteristics. This is because installing an emission gas analyzer on a ship has substantial economic burden, and, even if it is installed, the accuracy can be increased by a virtual sensor. Furthermore, data were obtained with and without operating the SCR (often mounted on ships to reduce NOx), which is a crucial facility to satisfy environment regulation. In this study, four types of datasets were created by adding cooling and electrical-related variables to the basic engine dataset to check whether it improves model performance or not; each of these datasets consisted of 15 to 26 variables as inputs. CO2 (%), NOx (ppm), and tEx (°C) were predicted from each dataset using an artificial neural network (ANN) model and a support vector machine (SVM) model with optimal hyperparameters selected by trial and error. The results confirmed that the SVM model performed better on smaller datasets, such as the one used in this study compared to the ANN model. Moreover, the dataset type, DaCE, which had both cooling and electrical-related variables added to the basic engine dataset, yielded the best overall prediction performance. When the performance of the SVM model was measured using the test data of a DaCE on both no-SCR mode and SCR mode, the RMSE (R2) of CO2 was between 0.1137% (0.8119) and 0.0912% (0.8975), the RMSE (R2) of NOx was between 17.1088 ppm (0.9643) and 13.6775 ppm (0.9776), and the RMSE (R2) of tEx was between 4.5839 °C (0.8754) and 1.5688 °C (0.9392).
Complications of Robotic Video-Assisted Thoracoscopic Surgery Compared to Open Thoracotomy for Resectable Non-Small Cell Lung Cancer
Authors: Zhang Oscar , Alzul Robert , Carelli Matheus , Melfi Franca , Tian David , Cao Christopher
Published: August 2022

(1) Background: Conventional open thoracotomy has been the accepted surgical treatment for resectable non-small cell lung cancer. However, newer, minimally invasive approaches, such as robotic surgery, have demonstrated similar safety and efficacy with potentially superior peri-operative outcomes. The present study aimed to quantitatively assess these outcomes through a meta-analysis. (2) Methods: A systematic review was performed using electronic databases to identify all of the relevant studies that compared robotic surgery with open thoracotomy for non-small cell lung cancer. Pooled data on the peri-operative outcomes were then meta-analyzed. (3) Results: Twenty-two studies involving 12,061 patients who underwent robotic lung resection and 92,411 patients who underwent open thoracotomy were included for analysis. Mortality rates and length of hospital stay were significantly lower in patients who underwent robotic resection. Compared to open thoracotomy, robotic surgery was also associated with significantly lower rates of overall complications, including atrial arrhythmia, post-operative blood transfusions, pneumonia and atelectasis. However, the operative times were significantly longer with robotic lung resection. (4) Conclusions: The present meta-analysis demonstrated superior post-operative morbidity and mortality outcomes with robotic lung resection compared to open thoracotomy for non-small cell lung cancer.
Application of the Optimal Parameter Geographic Detector Model in the Identification of Influencing Factors of Ecological Quality in Guangzhou, China
Authors: Zhang Maomao , Kafy Abdulla-Al , Ren Bing , Zhang Yanwei , Tan Shukui , Li Jianxing
Published: August 2022

The ecological environment is important for the survival and development of human beings, and objective and accurate monitoring of changes in the ecological environment has received extensive attention. Based on the normalized difference vegetation index (NDVI), wetness (WET), normalized differential build-up and bare soil index (NDBSI), and land surface temperature (LST), the principal component analysis method is used to construct a comprehensive index to evaluate the ecological environment’s quality. The R package “Relainpo” is used to estimate the relative importance and contribution rate of NDVI, WET, NDBSI, and LST to the remote sensing ecological index (RSEI). The optimal parameter geographic detector (OPGD) model is used to quantitatively analyze the influencing factors, degree of influence, and interaction of the RSEI. The results show that from 2001 to 2020, the area with a poor grade quality of the RSEI in Guangzhou decreased from 719.2413 km2 to 660.4146 km2, while the area with an excellent quality grade of the RSEI increased from 1778.8311 km2 to 1978.9390 km2. The NDVI (40%) and WET (35%) contributed significantly to the RSEI, while LST and NDBSI contributed less to the RSEI. The results of single factor analysis revealed that soil type have the greatest impact on the RSEI with a coefficient (Q) of 0.1360, followed by a temperature with a coefficient (Q) of 0.1341. The interaction effect of two factors is greater than that of a single factor on the RSEI, and the interaction effect of different factors on the RSEI is significant, but the degree of influence is not consistent. This research may provide new clues for the stabilization and improvement of ecological environmental quality.
Neoliberal Lakeside Residentialism: Real Estate Development and the Sustainable Utopia in Environmentally Fragile Areas
Authors: Hidalgo Rodrigo , Robles María Sarella , Alvarado Voltaire
Published: August 2022

This article exposes the central role played by neoliberal real estate development in the transformation of two lakeside cities in southern Chile. The concept of neoliberal lakeside residentialism addresses the ways in which commercialisation of the natural world in tourism hotspots is comprehensively reshaping the environmentally fragile Andean lake district. Specifically, we hypothesise that this green utopia is rapidly becoming a dystopia as a result of the aggregate effects of real estate development on environmental sustainability. In order to analyse these tensions, we conduct a case study in the districts of Villarrica and Pucón, both located within the Lake Villarrica watershed. There is evidence of territorial metamorphosis of the Araucanía Region as areas popular with tourists are increasingly being closed off by large-scale real estate operations. We find that this “anomalous” rate of urbanisation is indeed affecting sustainability in the territory, both environmental and social, concluding that regulation of these processes is needed in order to preserve both the natural and cultural wealth of the study area.
Tense and Aspect in a Spanish Literary Work and Its Translations
Authors: Mulder Gijs , Schoenmakers Gert-Jan , Hoenselaar Olaf , de Hoop Helen
Published: August 2022

This paper reports on a literary corpus study of four grammatical tenses across four European languages. The corpus consists of a selection of eight chapters from Javier Marías’s Spanish novel Así empieza lo malo ‘Thus bad begins’, and its translations to English, Dutch, and French. We annotated 1579 verb forms in the Spanish source text for tense, and, subsequently, their translations in the other languages, distinguishing between two registers within the novel, i.e., dialogue and narration. We found that the vast majority of the Spanish tenses are translated one-to-one to their counterparts in the three languages, especially in narration. In dialogue, we found several deviations, which we could partially account for within an Optimality Theoretic approach by appealing to the notion of markedness along two different typological dimensions, namely, tense (present versus past) and aspect (imperfective versus perfective).
Outcome after Prenatal Diagnosis of Trisomy 13, 18, and 21 in Fetuses with Congenital Heart Disease
Authors: Springer Stephanie , Karner Eva , Worda Christof , Grabner Maria Magdalena , Seidl-Mlczoch Elisabeth , Laccone Franco , Neesen Jürgen , Scharrer Anke , Ulm Barbara
Published: August 2022

Fetal congenital heart disease (CHD) is often associated with chromosomal abnormalities. Our primary aim was to assess stillbirth and neonatal mortality rates for pregnancies complicated by trisomies 13, 18, and 21 in the presence of CHD, from a single tertiary referral center during 2000–2020 in a retrospective cohort study. The secondary aims were to investigate maternal morbidity in these pregnancies, and to study the gestational or neonatal age when mortality occurred. Inclusion criteria were the prenatal diagnosis of at least one structural CHD, together with prenatally diagnosed fetal trisomy 13, 18, or 21. One-hundred and sixty patients with fetal trisomy 13 (14.4%), fetal trisomy 18 (28.8%), and fetal trisomy 21 (56.9%) were evaluated. In total, 98 (61.3%) families opted for the termination of pregnancy (TOP). Of the remaining 62 (38.8%) pregnancies, 16 (25.8%) resulted in intrauterine fetal death/death during delivery. Ten out of twenty-one (47.6%) infants with trisomy 13 or 18 were born alive. The livebirth rate was 87.8% (36/41) for infants with trisomy 21. Early neonatal death was observed in nine (19.6%) infants. Thirty-one (86.1%) infants with trisomy 21 survived the first year of life. These data may be helpful for counseling affected parents when the decision to terminate or continue the pregnancy should be considered.
Spinyhead Croaker Germ Cells Gene dnd Visualizes Primordial Germ Cells in Medaka
Authors: Xu Cong , Li Yu , Wen Zhengshun , Jawad Muhammad , Gui Lang , Li Mingyou
Published: August 2022

Spinyhead croaker (Collichthys lucidus) is an economically important fish suffering from population decline caused by overfishing and habitat destruction. Researches on the development of primordial germ cell (PGC) and reproduction biology were an emergency for the long-term conservation of the involved species. Dead end (dnd) gene plays an indispensable role in PGC specification, maintenance, and development. In the current study, we report the cloning and expression patterns of dnd in C. lucidus (Cldnd). RT-PCR analysis revealed that Cldnd was specifically expressed in both sexual gonads. In the ovary, Cldnd RNA was uniformly distributed in the oocytes and abundant in oogonia, and gradually decreased with oogenesis. A similar expression pattern was also detected in testis. Dual fluorescent in situ hybridization of Cldnd and Clvasa demonstrated that they almost had the same distribution except in oocytes at stage I, in which the vasa RNA aggregated into some particles. Furthermore, Cldnd 3′ UTR was sufficient to guide the Green Fluorescent Protein (GFP) specifically and stably expressed in the PGCs of medaka. These findings offer insight into that Cldnd is an evolutionarily conserved germline-specific gene and even a potential candidate for PGC manipulation in C. lucidus.
Increasing the Equilibrium Solubility of Meloxicam in Aqueous Media by Using Dimethyl Sulfoxide as a Cosolvent: Correlation, Dissolution Thermodynamics and Preferential Solvation
Authors: Tinjacá Darío A. , Martínez Fleming , Almanza Ovidio A. , Peña M. Ángeles , Jouyban Abolghasem , Acree William E.
Published: August 2022

Meloxicam is widely prescribed as an analgesic and anti-inflammatory drug in human therapeutics. Owing the very low aqueous solubility of meloxicam, this property has been studied in dimethyl sulfoxide (DMSO)-aqueous solvent systems at several temperatures from 273.15 to 313.15 K to expand the solubility database about analgesic drugs in mixed solvents. The flask shake method followed by ultraviolet-visible (UV-vis) spectrophotometry analysis were used for meloxicam solubility determinations. A number of cosolvency models, including the Jouyban–Acree model, were challenged for solubility correlation/prediction of this drug in these mixtures. The van’t Hoff and Gibbs equations were employed to calculate the apparent standard thermodynamic quantities relative to dissolution and mixing processes. The inverse Kirkwood–Buff integral method was employed for calculating the preferential solvation parameters of meloxicam by DMSO in the mixtures. Meloxicam solubility increases with increasing temperature and maximum solubilities are observed in neat DMSO at all temperatures studied. Dissolution processes were endothermic in all cases and entropy-driven in the composition interval of 0.40 ≤ x1 ≤ 1.00. A nonlinear enthalpy–entropy relationship was observed in the plot of enthalpy vs. Gibbs energy for drug transfer processes. Meloxicam is preferentially solvated by water in water-rich mixtures but preferentially solvated by DMSO in the composition interval of 0.21 < x1 < 1.00.