bluuebunny/crossref_metadata_2025 huggingface dataset:
Dectin-1/IL-15 Pathway Affords Protection against Extrapulmonary Aspergillus fumigatus Infection by Regulating Natural Killer Cell Survival
Authors: Yoshikawa Fábio S.Y. , Wakatsuki Maki , Yoshida Kosuke , Yabe Rikio , Torigoe Shota , Yamasaki Sho , Barber Glen N. , Saijo Shinobu
Published: January 2023

Aspergillus fumigatus is a ubiquitous, yet potentially pathogenic, mold. The immune system employs innate receptors, such as dectin-1, to recognize fungal pathogens, but the immunological networks that afford protection are poorly explored. Here, we investigated the role of dectin-1 in anti-A. fumigatus response in an experimental model of acute invasive aspergillosis. Mice lacking dectin-1 presented enhanced signs of inflammation, with increased production of inflammatory cytokines and neutrophil infiltration, quickly succumbing to the infection. Curiously, resistance did not require T/B lymphocytes or IL-17. Instead, the main effector function of dectin-1 was the preservation of the NK cell population in the kidneys by the provision of the cytokine IL-15. While the depletion of NK cells impaired host defense in wild-type mice, IL-15 administration restored antifungal responses in dectin-1-deficient mice. Our results uncover a new effector mechanism for dectin-1 in anti-Aspergillus defense, adding an alternative approach to understand the pathophysiology of this infection.
The Aryl Hydrocarbon Receptor Ligand 6-Formylindolo(3,2-b)carbazole Promotes Estrogen Receptor Alpha and c-Fos Protein Degradation and Inhibits MCF-7 Cell Proliferation and Migration
Authors: Cano-Sánchez José , Murillo-González Fátima E. , de Jesús-Aguilar Jannet , Cabañas-Cortés María Asunción , Tirado-Garibay Ana Carolina , Elizondo Guillermo
Published: January 2023

Introduction: Worldwide, breast cancer is the most common cancer in women and is the main cause of death among all neoplasia in this group. Luminal A breast cancer represents approximately 70% of all breast cancers and is treated with hormone therapies targeting estrogen receptor alpha (ERα). Unfortunately, patients develop drug resistance leading to recurrence of neoplasia due to estrogen-independent ERα reactivation. Therefore, it is crucial to identify new molecular targets downstream ERα signaling pathway that allows the implementation of better treatments to improve the outcome of breast cancer patients. Overexpression of c-Fos, an ERα gene target, has been associated with increased cell motility, malignancy, metastasis, and invasion while its neutralization results in decreased breast cancer tumorigenesis. The aryl hydrocarbon receptor (AHR) ligands halogenated and polycyclic aromatic hydrocarbons, highly toxic compounds, down regulate c-Fos and ERα levels. The present study aimed to evaluate whether 6-formylindolo(3,2-b)carbazole (FICZ), a no toxic AHR agonist, modifies c-Fos levels in MCF-7 mammary carcinoma cells as well as to determine its effects on cell proliferation and migration. In addition, the possible mechanism through which FICZ mediates c-Fos levels in MCF-7 cells was investigated. Methods: Initially, the effect of FICZ on c-Fos mRNA and protein levels in MCF-7 cells, untreated or treated with estradiol, was evaluated by qPCR and Western blot. 2,3,7,8-Tetrachloro-dibenzo-p-dioxin, an AHR prototype agonist, was used as a positive control. Next, we examined the effect of FICZ on MCF-7 cell proliferation and migration by cell counting, MTT, 3H-thymidine incorporation, and scratch-wound assays. Finally, the involvement of proteasome 26S on ERα and c-Fos protein degradation was investigated by the use of MG132 and Western blot. Results: The data show that FICZ treatment downregulates c-Fos mRNA and protein levels, most likely by promoting ERα proteasome degradation, blocking MCF-7 cell proliferation and migration. The results also demonstrate that liganded ERα was required for FICZ-mediated ERα degradation. Conclusions: Activation of AHR results in a decreased MCF-7 cell proliferation and migration by ERα and c-Fos down regulation. Targeting AHR might be a promising therapy for breast cancer treatment, particularly when estrogen-independent ERα reactivation presents.
Effects of a Sound Intervention on Physical and Emotional Well-Being in Patients with Cancer: A Prospective Randomized Trial
Authors: Hohneck Anna , Meissner Rosa , Reyser Christina , Heinemann Lara , Christians Kathrin , Merx Kirsten , Weingärtner Simone , Mavratzas Athanasios , Schulte Nadine , Burkholder Iris , Hofmann Wolf-Karsten , Hofheinz Ralf-Dieter
Published: January 2023

Aim: Cancer remains a disease with a significant impact on morbidity and mortality but also on quality of life. This prospective randomized pilot study investigated the effects of a sound intervention on physical and emotional well-being in outpatients with cancer. Methods: Two self-applied sound interventions were used for this purpose, either active “music playing” with a body monochord or passive sound intervention with headphones to listen to a given music compilation. Interventions were carried out over a period of 4 weeks for at least 15 min in the evening before bedtime. The following self-assessment questionnaires were completed both at baseline and after 4 weeks to evaluate the response: the Pittsburgh Sleep Quality Index (PSQI), the European Organization for Research and Treatment of Cancer (EORTC) Quality of Life Questionnaire (QLQ-C30), Visual Analogue Scale (VAS) for pain and fatigue, and the Fear of Progression (FoP) questionnaire. Primary endpoint of this exploratory trial was to describe the rate of patients with improvement in at least one dimension without worsening of any other. Results: 73 patients (29 male, 44 female) were included in the study and randomized to either active (n = 34, 47%) or passive sound intervention (n = 39, 53%). Median age was 52.0 years (range 21–79). Fourteen patients (19%) stated that they were musically active. The sound intervention was carried out on a median of 26 days (range 5–28). A higher percentage of patients in the passive group reached the primary endpoint: n = 15 (39%) versus n = 9 (27%). Response differences in favour of the passive group were seen with the VAS fatigue and with QLQ-30 questionnaires. Overall, an improvement in QLQ-30 questionnaire was seen in 12 patients (31%) in the passive group versus 3 patients (9%). Moreover, sound intervention significantly improved social functioning and shortness of breath in the passive group according to QLQ-C30. Significant improvements were also noticed in the passive group in terms of affective reactions as a domain of the FoP questionnaire. No effects on pain or sleep quality could be observed. Conclusion: A 4-week self-administered sound intervention was feasible in outpatients suffering from cancer. Using a panel of 5 questionnaires, passive sound interventions appeared to be more likely to positively influence patient-reported outcomes. In particular, a positive impact was documented in social functioning and fatigue.
Triosephosphate Isomerase Deficiency: E105D Mutation in Unrelated Patients and Review of the Literature
Authors: Selamioğlu Arzu , Karaca Meryem , Balcı Mehmet Cihan , Körbeyli Hüseyin Kutay , Durmuş Aslı , Yıldız Edibe Pembegül , Karaman Serap , Gökçay Gülden Fatma
Published: January 2023

Introduction: Chronic haemolytic anaemia, increased susceptibility to infections, cardiomyopathy, neurodegeneration, and death in early childhood are the clinical findings of triosephosphate isomerase (TPI) deficiency, which is an ultra-rare disorder. The clinical and laboratory findings and the outcomes of 2 patients with TPI deficiency are reported, with a review of cases reported in the literature. Case Presentation: Two unrelated patients with haemolytic anaemia and neurologic findings who were diagnosed as having TPI deficiency are presented. Neonatal onset of initial symptoms was observed in both patients, and the age at diagnosis was around 2 years. The patients had increased susceptibility to infections and respiratory failure, but cardiac symptoms were not remarkable. Screening for inborn errors of metabolism revealed a previously unreported metabolic alteration determined using tandem mass spectrometry in acylcarnitine analysis, causing elevated propionyl carnitine levels in both patients. The patients had p.E105D (c.315G>C) homozygous mutations in the TPI1 gene. Although severely disabled, both patients are alive at the ages of 7 and 9 years. Discussion: For better management, it is important to investigate the genetic aetiology in patients with haemolytic anaemia with or without neurologic symptoms who do not have a definitive diagnosis. The differential diagnosis of elevated propionyl carnitine levels using tandem mass spectrometry screening should also include TPI deficiency.
Brief Report: Protective or Risk Factor? Aftermath Effects of Perceived Social Support on Embitterment during the COVID-19 Pandemic
Authors: Koroma Dennis , Znoj Hansjörg , Soravia Leila M.
Published: January 2023

There are indications for a raise in embitterment during the COVID-19 pandemic. As embitterment is related to felt social exclusion, pessimism, and a proneness toward COVID-19-related conspiracy beliefs, embitterment may be a key factor to consider in the current pandemic. However, perceived social support (PSS), which is associated with hope during the COVID-19 pandemic could serve as an important resilience factor for bitterness. We therefore investigated the effects of PSS on embitterment in aftermath of the second wave of the COVID-19 pandemic. 52 individuals participated in our follow-up online study in October 2020 (T1) and May 2021 (T2). Embitterment (measured with the Berne Embitterment Inventory) and PSS were repeatedly assessed at T1 and T2. A double moderation analysis was conducted, using the PROCESS macro to examine the effects of embitterment, PSS, and age on embitterment 6 months later. The results suggest that the prediction of embitterment at T2 by embitterment at T1 was altered by changes in PSS. Here, in particular participants with high embitterment at T1 and a gain in PSS had higher embitterment scores at T2, whereas participants with low embitterment were found to have lower embitterment scores. This interaction effect was further influenced by the age of the participants, where the effects were more pronounced among middle-aged participants than younger participants. As embittered individuals seem to grow in bitterness with a gain in PSS, support is found for the notion that embittered individuals seeking allies supporting their mindset.
Accuracy of FDG-PET/CT for Response Evaluation of Muscle-Invasive Bladder Cancer following Neoadjuvant or Induction Chemotherapy
Authors: Fitoussi Olivier , Roche Jean Baptiste , Riviere Julien , Wallerand Hervé , Poulain Jean Eude , Gordien Pierre , Galland Sigolène , Henriques Bénédicte , Dupin Camille , Vincent Muriel , Kuratle Thomas , Saffarini Mo , Ramos-Pascual Sonia
Published: January 2023

Introduction: The aim of the study was to confirm the diagnostic accuracy of a second FDG-PET/CT following neoadjuvant or induction chemotherapy (NAIC) prior to radical cystectomy for patients with localized muscle-invasive bladder cancer (MIBC). Methods: Retrospective review of 62 consecutive patients with MIBC, that had a first FDG-PET/CT between April 2016 and September 2021. Patients then underwent NAIC, followed by a second FDG-PET/CT and radical cystectomy. Patients with no hypermetabolism in the bladder and lymph nodes on the second FDG-PET/CT were considered metabolic complete responders, while patients with no evidence of residual disease on histopathology were considered pathologic complete responders. The accuracy of the second FDG-PET/CT to distinguish complete responders from patients with residual disease was calculated, with histopathology as gold standard. Results: Of 62 patients, 1 was lost to follow-up, 5 died before radical cystectomy, 5 had delay >2 months between the second FDG-PET/CT and radical cystectomy, and 6 did not undergo radical cystectomy and instead underwent alternative treatment. The study cohort comprised 45 patients, 39 males and 6 females, with an age of 66 ± 6 years. In comparison to histopathology, FDG-PET/CT provided (i) sensitivity of 95% and specificity of 42%, for the overall disease; (ii) sensitivity of 100% and specificity of 36%, for the primary tumor only; and (iii) sensitivity of 97% and specificity of 30%, for the lymph nodes only. Conclusion: FDG-PET/CT has over 95% sensitivity for distinguishing complete responders from patients with residual disease. Thus, FDG-PET/CT can be used for early response evaluation following NAIC to identify patients that did not completely respond to chemotherapy and may require alternative treatment pathways.
Nasal Septal Perforation Secondary to Graft-Versus-Host Disease
Authors: Hoover Will P. , Wilson Caleb P. , Hasnie Sukaina , McKinney Kibwei A.
Published: January 2023

Nasal septal perforation is a full-thickness defect of the nasal septum. There are many described etiologies of nasal septal perforation, including trauma, infectious, neoplastic, iatrogenic, and autoimmune. Graft-versus-host disease (GVHD) is a common and potentially life-threatening complication that can occur after an allogenic transplant. GVHD can result in the development of autoantibodies that lead to granulomatous inflammation with necrotizing vasculitis, causing perforation of the nasal septum. In this report, we describe a patient with nasal septal perforation secondary to GVHD and hope to provide novel insights into the association of GVHD and nasal septal perforation.
Ungewöhnliche akute Hepatitisfälle bei Kindern 2022 – Evidenz für eine immunvermittelte Erkrankung – ein Fall von Long-COVID?
Authors: Bengsch Bertram
Published: January 2023

Objectives: Severe acute respiratory syndrome coronavirus 2, the novel coronavirus responsible for coronavirus disease (COVID-19), has been a major cause of morbidity and mortality worldwide. Gastrointestinal and hepatic manifestations during acute disease have been reported extensively in the literature. Post-COVID-19 cholangiopathy has been increasingly reported in adults. In children, data are sparse. Our aim was to describe pediatric patients who recovered from COVID-19 and later presented with liver injury. Methods: This is a retrospective case series study of pediatric patients with post-COVID-19 liver manifestations. We collected data on demographics, medical history, clinical presentation, laboratory results, imaging, histology, treatment, and outcome. Results: We report 5 pediatric patients who recovered from COVID-19 and later presented with liver injury. Two types of clinical presentation were distinguishable. Two infants aged 3 and 5 months, previously healthy, presented with acute liver failure that rapidly progressed to liver transplantation. Their liver explant showed massive necrosis with cholangiolar proliferation and lymphocytic infiltrate. Three children, 2 aged 8 years and 1 aged 13 years, presented with hepatitis with cholestasis. Two children had a liver biopsy significant for lymphocytic portal and parenchyma inflammation, along with bile duct proliferations. All 3 were started on steroid treatment; liver enzymes improved, and they were weaned successfully from treatment. For all 5 patients, extensive etiology workup for infectious and metabolic etiologies was negative. Conclusions: We report 2 distinct patterns of potentially long COVID-19 liver manifestations in children with common clinical, radiological, and histopathological characteristics after a thorough workup excluded other known etiologies.
Long Non-Coding RNA BCAR4 Promotes Oxaliplatin Resistance in Colorectal Cancer by Modulating miR-484-3p/RAB5C Expression
Authors: Li Xianting , Chen Xiaojiao , Fu Chendong , Xie Ming , Ouyang Shurui
Published: January 2023

Background: Oxaliplatin-based chemotherapy resistance is a major cause of recurrence in patients with colorectal cancer (CRC). Increasing evidence indicates that lncRNA BCAR4 is involved in the occurrence and development of various cancers. However, the effect of BCAR4 on CRC chemotherapy resistance remains unclear. Methods: Real-time quantitative PCR and Western blotting were used to detect the expression levels of gene and protein, respectively. The role of BCAR4 in drug resistance was evaluated by cell viability and apoptosis experiments. Luciferase reporter assay and Western blot analysis confirmed the relationship between BCAR4, miR-483-3p, and RAB5C. Results: Luciferase reporter assay and Western blotting analysis confirmed the relationship among BCAR4, miR-483-3p, and RAB5C. The results showed that the expression levels of BCAR4 and RAB5C were increased in CRC tumor tissue. The expression levels of BCAR4 were increased in patients with chemotherapy resistance. Functional analysis showed that knockdown of BCAR4 reduced the expression levels of proteins related to stemness, decreased the activity of cells, and promoted apoptosis of CRC cells, while overexpression of RAB5C reversed these effects. Moreover, the results showed that BCAR4 promoted oxaliplatin resistance by inhibiting cell apoptosis. Mechanistically, BCAR4 sponged miR-483-3p and promoted the expression of RAB5C. Knockdown of BCAR4 reduced tumor size and enhanced cell sensitivity to oxaliplatin in vivo. Conclusion: The results suggested that BCAR4/miR-483-3p/RAB5C axis has the potential to be explored as a novel therapeutic target for CRC treatment.
Profile of milk consumers and determinants of A2A2 milk consumption in the Tocantins State
Authors: Oliveira Rodolfo Olinto Rotoli Garcia , Souza Ana Beatriz Bezerra , Folhas Raryanne Dias , Silva Marcelo Corrêa da , Sobreira Itallo Romero Marques , Pereira Mariane Santos , Ferraz José Bento Sterman , Carvalho Minos Esperandio de , Nepomuceno Leandro Lopes , Santos Helcileia Dias , Garcia José Américo Soares , Ferreira Jorge Luís
Published: January 2023

The dairy industry diversifies products with organoleptic, dietary, functional, and nutritional properties according to consumer demands and welfare. This study aimed to examine the profile of consumers, data on milk consumption, knowledge, and familiarity with A2A2 milk in the Tocantins State, Brazil. A qualitative survey was carried out through a questionnaire applied to 389 people, distributed in 34 municipalities. Regarding the consumption of dairy products, 21.31% of participants described having some type of discomfort when consuming them. Among participants, 46.27% consumed one to two liters, 28.28% two to four liters, and 25.45% more than four liters per week. Milk was the main dairy product consumed, according to 59.13% of participants. Of all respondents, 76.10% pay little or no attention to the information on the label, versus 23.90% who did not. Regarding A2A2 milk, 40.36% possessed some knowledge about it, while 59.64% did not. 42.93% and 30.59% of participants stated they would be willing to pay over 10% or 50% more, respectively, whereas 24.94% would not pay any additional amount on the price of A2A2 milk. Willingness to pay a low additional amount was related to consumers who heard of, but knew little about this product, whereas the likelihood of paying higher additional amounts was associated with respondents who heard and know about the product.
The Circadian Biology of Heart Failure
Authors: El Jamal Nadim , Lordan Ronan , Teegarden Sarah L. , Grosser Tilo , FitzGerald Garret
Published: January 2023

Driven by autonomous molecular clocks that are synchronized by a master pacemaker in the suprachiasmatic nucleus, cardiac physiology fluctuates in diurnal rhythms that can be partly or entirely circadian. Cardiac contractility, metabolism, and electrophysiology, all have diurnal rhythms, as does the neurohumoral control of cardiac and kidney function. In this review, we discuss the evidence that circadian biology regulates cardiac function, how molecular clocks may relate to the pathogenesis of heart failure, and how chronotherapeutics might be applied in heart failure. Disrupting molecular clocks can lead to heart failure in animal models, and the myocardial response to injury seems to be conditioned by the time of day. Human studies are consistent with these findings, and they implicate the clock and circadian rhythms in the pathogenesis of heart failure. Certain circadian rhythms are maintained in patients with heart failure, a factor that can guide optimal timing of therapy. Pharmacologic and nonpharmacologic manipulation of circadian rhythms and molecular clocks show promise in the prevention and treatment of heart failure.
Using Model Cloud Information to Reassign Low-Level Atmospheric Motion Vectors in the ECMWF Assimilation System
Authors: Lean Katie , Bormann Niels
Published: January 2023

This paper investigates the use of model cloud information in the assimilation of low-level atmospheric motion vectors (AMVs) in the ECMWF global data assimilation system, with the aim to characterize and address issues encountered in the assimilation of these observations. An analysis of background departure statistics (comparison of observations with the model background) shows that AMVs placed above the model cloud show larger deviations from the model background relative to those placed unrealistically close to the surface. Reassigning the pressure of AMVs diagnosed above the model cloud layer to either the model cloud top, cloud base, or average pressure leads to improvements in root-mean-square vector difference (RMSVD) and speed bias against the background wind fields. In assimilation experiments, reassigning AMVs placed above the model cloud to the model cloud top, cloud base, or average pressure results overall in a positive impact on subsequent forecasts. The reassignment to an average model cloud pressure performs best in this respect, and this approach has been implemented in the operational ECMWF system in October 2021.
Comparing the Contributions of Temperature and Salinity Changes to the AMOC Decline at 26.5°N
Authors: Lu Wenbo , Zhou Chun , Zhao Wei , Zhang Cunjie , Geng Tao , Xiao Xin
Published: January 2023

At 26.5°N in the North Atlantic, a continuous transbasin observational array has been established since 2004 to detect the strength of the Atlantic meridional overturning circulation. The observational record shows that the subtropical Atlantic meridional overturning circulation has weakened by 2.5 ± 1.5 Sv (as mean ± 95% interval; 1 Sv ≡ 106 m3 s−1) since 2008 compared to the initial 4-yr average. Strengthening of the upper southward geostrophic transport (with a 2.6 ± 1.6 Sv southward increase) derived from thermal wind dominates this Atlantic meridional overturning circulation decline. We decompose the geostrophic transport into its temperature and salinity components to compare their contributions to the transport variability. The contributions of temperature and salinity components to the southward geostrophic transport strengthening are 1.0 ± 2.5 and 1.6 ± 1.3 Sv, respectively. The variation of salinity component is significant at the 95% confidence level, while the temperature component’s variation is not. This result highlights the vital role that salinity plays in the subtropical Atlantic meridional overturning circulation variability, which has been overlooked in previous studies. We further analyze the geostrophic transport variations and their temperature and salinity components arising from different water masses, which shows that a warming signal in Labrador Sea Water and a freshening signal in Nordic Sea Water are two prominent sources of the geostrophic transport increase. Comparison of the temperature and salinity records of the 26.5°N array with the upstream records from repeated hydrographic sections across the Labrador Sea suggests that these thermohaline signals may be exported from the subpolar Atlantic via the deep western boundary current.
Scripps Argo Trajectory-Based Velocity Product: Global Estimates of Absolute Velocity Derived from Core, Biogeochemical, and Deep Argo Float Trajectories at Parking Depth
Authors: Zilberman N. V. , Scanderbeg M. , Gray A. R. , Oke P. R.
Published: January 2023

Global estimates of absolute velocities can be derived from Argo float trajectories during drift at parking depth. A new velocity dataset developed and maintained at Scripps Institution of Oceanography is presented based on all Core, Biogeochemical, and Deep Argo float trajectories collected between 2001 and 2020. Discrepancies between velocity estimates from the Scripps dataset and other existing products including YoMaHa and ANDRO are associated with quality control criteria, as well as selected parking depth and cycle time. In the Scripps product, over 1.3 million velocity estimates are used to reconstruct a time-mean velocity field for the 800–1200 dbar layer at 1° horizontal resolution. This dataset provides a benchmark to evaluate the veracity of the BRAN2020 reanalysis in representing the observed variability of absolute velocities and offers a compelling opportunity for improved characterization and representation in forecast and reanalysis systems.

Significance Statement

The aim of this study is to provide observation-based estimates of the large-scale, subsurface ocean circulation. We exploit the drift of autonomous profiling floats to carefully isolate the inferred circulation at the parking depth, and combine observations from over 11 000 floats, sampling between 2001 and 2020, to deliver a new dataset with unprecedented accuracy. The new estimates of subsurface currents are suitable for assessing global models, reanalyses, and forecasts, and for constraining ocean circulation in data-assimilating models.
The History of Dictatorship: Custom, Authority, and Power in Precolonial and Colonial Uganda
Authors: Sseremba Yahya
Published: January 2023

Intervening in the enduring debate on the origins of the African state, this article examines the processes of producing custom in the Ugandan societies of precolonial Bunyoro and colonial Toro to trace the development of despotism. The participatory nature of generating customary truth in Bunyoro before European domination reflects the diffusion of power in a manner that hindered absolute rule. On the contrary, in colonial Toro, the inclusive mechanisms for making custom gave way to customary law produced by the colonial government and its native chiefs. This monopoly to determine customary law disguised as custom constituted the heart of the despotism of Toro Native Authority. Derivatively, the Rwenzururu resistance against Toro domination equally assumed a despotic character because it organised itself along the logic of the authority it confronted. The study interrogates the resurgent literature that associates the contemporary African state with precolonial history.
Southeastern Surgical Congress Presidential Address: “Finding Your Own Unique Place Within the House of Surgery”
Authors: Richmond Bryan K.
Published: January 2023

The term the “House of Surgery” is often used in conversation, but the origin of the phrase is not well known to most. In the following article, the origin of the phrase is explained, as is its relevance to the maintenance of a strong surgical community in today’s world. In addition, examples are provided as to how each surgeon can find their own unique role in the House of Surgery, with the goal being to increase understanding and collaboration tween surgeons in all types of practice settings
Evaluation of the new Abbott®Alinity assay for the measurement of thyroglobulin antibodies in patients with differentiated thyroid carcinoma
Authors: Garcia Cano Ana M , Rosillo Coronado Marta , Alcázar García Laura , Gómez Lozano Ana , Carrasco Sayalero Angela , Rodriguez Torres Argeme
Published: January 2023

Background

Measurement of antithyroglobulin antibodies (TgAb) is important in patients with differentiated thyroid carcinoma (DTC) with total thyroidectomy. These patients are monitored based on serum thyroglobulin (Tg) levels. TgAb is known to interfere with the measurement of Tg by immunoassay. This study evaluates a new methodology for the measurement of TgAb Alinity Abbott® and the concordance with other methods (first and second generation Advia Centaur Systems Siemens® and Phadia 250 Thermofisher®).

Methods

The technical characteristics of Alinity TgAb measurement methodology were analysed, with imprecision and repeatable studies. In order to assess concordance, a minimum of 69 and a maximum of 76 samples from patients with DTC and total thyroidectomy were processed in parallel by several TgAb measurement methodologies. Agreement rates were determined using kappa statistics. The correlation between the four methods was examined pairwise using McNemar test analysis.

Results

The coefficients of variation (standard deviation as a percentage of the mean % CV) for the Alinity Abbott kit reagent TgAb were within 10% included the functional sensitivity. On the other hand, the concordance analysis with the kappa index concluded substantial agreement. The McNemar test showed a significant difference between Alinity versus Centaur second generation (difference 8.33%, CI 95% 0.68–8.33, p = .0313).

Conclusion

The new methodology for the measurement of TgAb meets the imprecision standards while presenting an adequate concordance agreement with other methodologies available in laboratories. It is important to define the functional sensitivity when reporting results so that they are as reliable as possible.
Developing and validating a highly sensitive platelet clump detection model for the Sysmex haematology analyser
Authors: Fang Kui , Chen Xiling , Dong Zheqing , Xu Peng
Published: January 2023

Background

Mainstream haematology analysers (HAs) are reported to have low detection sensitivity for platelet clumps. In this study, a deep learning (DL) algorithm, convolutional neural network (CNN), was implemented to detect platelet clumps.

Methods

Adenosine diphosphate (ADP) was used to induce platelet aggregation to mimic platelet clumps detected (PCD) samples. Six types of leukocyte scattergrams were collected from the Sysmex XN-10. Then, multiple CNNs were trained and validated by scattergrams in a fivefold cross-validation (CV) method. Finally, the CNN model with the best CV accuracy was tested with practical routine work samples.

Results

A total of 386 samples (190 PCD and 196 negative samples) and 4253 samples (150 PCD and 4103 negative samples) were eligible for CNN training and practical test, respectively. The CNN with the highest CV accuracy was trained by using scattergrams of side scatter (SSC) vs. forward scatter (FSC) from the white count and nucleated red blood cells (WNR) channel, whose mean area under the curve (AUC), accuracy, specificity and sensitivity were 0.968, 0.940, 0.937 and 0.942, respectively, in the CV. In the practical test, the AUC, accuracy, specificity and sensitivity of the CNN were 0.916, 0.961, 0.860 and 0.965, respectively. The dispersed spots presenting around the leucocytes in the WNR channel may be a sign of platelet clumping.

Conclusions

This study demonstrates that the CNN algorithms can identify platelet clumps based on optical information from dedicated leukocyte channels and has a higher ability to detect platelet clumps than the XN-10 device’s internal algorithm under practical circumstances.
Algorithmic autobiographies and fictions: A digital method
Authors: Bishop Sophie , Kant Tanya
Published: January 2023

In this article, we outline an original, creative method for capturing the multifaceted ways in which digital technologies shape social life. We outline a framework for engaging participants in creative writing and drawing techniques to support ‘meeting and greeting’ their ‘algorithmic selves’. Algorithmic selves offer datafied reflections of individuals’ social media use, represented through platform approximated advertising categories. These categories include identities, such as ‘female’ or ‘male’, and marketing interests as ‘dog lovers’, ‘first time buyers’ or ‘feminists’. Our method builds on Les Back’s calls for ‘a more artful form of sociology’ that is able to think with technology. By using algorithmic selves to mobilise creative enquiry in this way, we argue that researchers can better discern how technology users make sense of their data, the ways in which identity can be co-constructed by social media platforms, and how our interactions with technology ultimately shape social lives in meaningful and highly affective ways. Our method offers a craft-based framework for understanding imaginations, associations and connections with data profiling, and making these understandings available for participant reflection and researcher analysis. This method can also support research participants in taking creative ownership and building agency around their interactions with social media platforms.
Work, boredom and rhythm in the time of COVID-19
Authors: Nash Louise , Lyon Dawn
Published: January 2023

This article uses Henri Lefebvre’s Rhythmanalysis as a foundational text for researching boredom, and offers a critical analysis of UK-based media commentaries about boredom and homeworking written during 2020 and 2021. We situate the discussion within the rhythmic rupture caused by the COVID-19 pandemic and foreground rhythm as a lens for understanding reported experiences and reflections on boredom and work. For non-essential workers, lockdown offered an opportunity to reconfigure working lives away from the constraints of commutes and everyday work settings, yet our findings highlight the narrative representation and experience of a particular type of boredom and inertia known as acedia. The analysis discusses the presence of acedia and absence of rhythm across three themes: acedia and being stuck in time and space; embodiment, movement and rhythm; and the relationship between the present and the future. We conclude by considering what the experience of boredom might mean for how we reconceptualise our post-pandemic working lives.
Frugal indigenous vessel loop made from a surgical glove
Authors: Agarwal Pawan , Shiva Bharath , Sharma Dhananjaya
Published: January 2023

We report a simple cost effective alternative to commercially available vessel loops made from sterilized surgical gloves for retraction of vital structures during vascular trauma, micro-vascular, micro-neural surgery.
Intravenous immunoglobulin in the management of neonatal sepsis: A randomised controlled trial
Authors: Rizvi Mohammad Qaim , Singh Mukesh Vir , Mishra Nandita , Shrivastava Anubha , Maurya Manisha , Siddiqui Shahid Akhtar
Published: January 2023

Sepsis is a leading cause of neonatal mortality and morbidity in low and middle-income countries. We designed a double-blinded randomised controlled trial in a neonatal intensive care unit (NICU) of a tertiary care teaching hospital to determine the role of intravenous immunoglobulin (IVIG) in decreasing hospital stay. Eighty neonates with clinical features of sepsis were enrolled in the study and placebo groups to receive 500 mg/kg of IVIG for three consecutive days or a placebo. The primary outcome measure was duration of hospital stay in days. The babies in both groups were comparable in terms of birth weight, gestation and sex distribution. There was no significant difference in duration of hospital stay (days) in the study and placebo groups. We found that treatment with IVIG did not shorten the duration of hospital stay in our setting.
Intergenerational Model of Financial Satisfaction and Parent-Child Financial Relationship
Authors: Zupančič Maja , Poredoš Mojca , Lep Žan
Published: January 2023

The study examined an intergenerational model of financial socialization and its outcomes that connects parents’ and their children’s self-perceived financial learning outcomes to satisfaction with financial management and parent-child financial relationships. The conceptual model was based on models of financial socialization processes contributing to healthy financial development of emerging adult students (Shim et al., 2010; Sirsch et al., 2020), but extended the links across two generations within the same family. Considering perspectives of both generations in a single model, it was tested in a sample of 482 pairs of Slovenian first-year university students and one of their parents. Structural equation modeling revealed that parental healthy financial learning outcomes (knowledge, behavioral control, behavior) shaped their children’s positive financial development (financial learning outcomes and satisfaction) and promoted the parents’ satisfaction with financial management. In turn, both the students’ and the parents’ financial management satisfaction positively predicted a joint measure of satisfaction with parent-child financial relationship. Similar links of financial learning outcomes to satisfaction with financial management and parent-child financial relationships were observed for both generations, even though parents and their children were financially socialized under different socioeconomic conditions.
Parents’ vocabulary instruction with preschoolers during shared book reading
Authors: Olszewski Arnold , Hood Rachel Lynell
Published: January 2023

Dialogic reading during shared book reading between adults and children is an effective way to promote vocabulary acquisition. However, there is limited research on what strategies parents are spontaneously using during book reading sessions, which are important to understand for optimizing parent training in dialogic reading. The current study investigates what strategies parents are currently using to teach vocabulary to their children and what parts of speech and tier of vocabulary they choose to discuss. The current study recruited low-income parents and their preschool-age (42–65 months), typically developing children from Head Start classrooms in the United States. Twenty-seven adults filmed themselves at home reading Giraffes Can't Dance by Giles Andreae and Guy Parker-Rees to their child, given the instruction to read as they normally would. The videos were coded looking at a dialogic reading framework: CROWD and PEER. Child behaviors and the vocabulary discussed were included in the coding. Results show that although there is a large amount of variability between parents in their use of strategies during shared book reading, in general, parents are using very few dialogic reading strategies to explicitly teach vocabulary. However, parents’ use of dialogic reading strategies was significantly correlated with child extratextual talk. When they did provide instruction, parents discussed mostly tier 1 nouns and verbs, rather than tier 2 words which are necessary for academic success. These results reinforce the need for parent training on interactive reading strategies.
Spontaneous vasomotion propagates along pial arterioles in the awake mouse brain like stimulus-evoked vascular reactivity
Authors: Munting Leon P , Bonnar Orla , Kozberg Mariel G , Auger Corinne A , Hirschler Lydiane , Hou Steven S , Greenberg Steven M , Bacskai Brian J , van Veluw Susanne J
Published: January 2023

Sensory stimulation evokes a local, vasodilation-mediated blood flow increase to the activated brain region, which is referred to as functional hyperemia. Spontaneous vasomotion is a change in arteriolar diameter that occurs without sensory stimulation, at low frequency (∼0.1 Hz). These vessel diameter changes are a driving force for perivascular soluble waste clearance, the failure of which has been implicated in neurodegenerative disease. Stimulus-evoked vascular reactivity is known to propagate along penetrating arterioles to pial arterioles, but it is unclear whether spontaneous vasomotion propagates similarly. We therefore imaged both stimulus-evoked and spontaneous changes in pial arteriole diameter in awake, head-fixed mice with 2-photon microscopy. By cross-correlating different regions of interest (ROIs) along the length of imaged arterioles, we assessed vasomotion propagation. We found that both during rest and during visual stimulation, one-third of the arterioles showed significant propagation (i.e., a wave), with a median (interquartile range) wave speed of 405 (323) µm/s at rest and 345 (177) µm/s during stimulation. In a second group of mice, with GCaMP expression in their vascular smooth muscle cells, we also found spontaneous propagation of calcium signaling along pial arterioles. In summary, we demonstrate that spontaneous vasomotion propagates along pial arterioles like stimulus-evoked vascular reactivity.
Xanthine oxidase mediates chronic stress-induced cerebrovascular dysfunction and cognitive impairment
Authors: Burrage Emily N , Coblentz Tyler , Prabhu Saina S , Childers Ryan , Bryner Randy W , Lewis Sarah E , DeVallance Evan , Kelley Eric E , Chantler Paul D
Published: January 2023

Xanthine oxidase (XO) mediates vascular function. Chronic stress impairs cerebrovascular function and increases the risk of stroke and cognitive decline. Our study determined the role of XO on stress-induced cerebrovascular dysfunction and cognitive decline. We measured middle cerebral artery (MCA) function, free radical formation, and working memory in 6-month-old C57BL/6 mice who underwent 8 weeks of control conditions or unpredictable chronic mild stress (UCMS) with or without febuxostat (50 mg/L), a XO inhibitor. UCMS mice had an impaired MCA dilation to acetylcholine vs. controls (p < 0.0001), and increased total free radical formation, XOR protein levels, and hydrogen peroxide production in the liver compared to controls. UCMS increased hydrogen peroxide production in the brain and cerebrovasculature compared to controls. Working memory, using the y-maze test, was impaired (p < 0.05) in UCMS mice compared to control mice. However, blocking XO using febuxostat prevented the UCMS-induced impaired MCA response, while free radical production and hydrogen peroxide levels were similar to controls in the liver and brain of UCMS mice treated with febuxostat. Further, UCMS + Feb mice did not have a significant reduction in working memory. These data suggest that the cerebrovascular dysfunction associated with chronic stress may be driven by XO, which leads to a reduction in working memory.
Experiences of Sentencing and the Pains of Punishment: Prisoners’ Perspectives
Authors: Atkins Deirdre , Maguire Niamh , Cleere Geraldine
Published: January 2023

Although sentencing is often described as a human process, the subjective experiences of those subject to sentencing are seldom discussed or highlighted as an important source of guidance for how sentencing might be made more fair, consistent, or proportionate. Tyler’s work on the links between experiences of procedural justice and perceptions of legitimacy in the criminal justice system show that how people are treated during sentencing and/or when serving their sentence matters in that it impacts their long-term compliance with the law. However, we suggest here that it may not only be long-term compliance that is impacted; subjective experiences of imprisonment, in terms of the pains of imprisonment, may also be exacerbated for those whose experiences of the sentencing process are predominantly negative. This article draws on 37 in-depth interviews with Irish prisoners that explored their subjective experiences of their own sentencing in court and how this related to their subjective experiences of their prison sentences. Those who felt they had received unreasonably harsh or unfair sentences, or who felt they were effectively excluded from the sentencing process, were more likely to experience specific pains and increased salience of punishment. The article concludes by arguing that these findings have a role to play in educating sentencers about how their treatment of convicted persons during sentencing can have meaningful, long-term consequences on the subjective experiences of those serving prison sentences.
Ankle Pain After Medial Opening-Wedge High Tibial Osteotomy in Patients With Knee Osteoarthritis and Concurrent Ankle Osteoarthritis
Authors: Kim Man Soo , Kim Jae Jung , Kang Ki Ho , Ihm Joon Soo , In Yong
Published: January 2023

Background:

Medial opening-wedge high tibial osteotomy (MOWHTO) in patients with varus knee osteoarthritis (OA) causes changes to ankle and hindfoot alignment. However, the compensatory ability of the ankle and hindfoot varies according to the severity of ankle OA.

Purpose:

To investigate whether the changes in ankle symptoms and ankle and hindfoot alignments differ after MOWHTO according to the severity of preoperative ankle OA.

Study design:

Case-control study; Level of evidence, 3.

Methods:

The data of 130 patients who were followed for ≥4 years were reviewed. Patients were classified into 2 groups according to their severity of ankle OA: group 1, modified Kellgren-Lawrence grade 0 and 1; group 2, grade ≥2. Four radiographic parameters were examined to evaluate ankle alignment: tibial plafond inclination, talar tilt, talar inclination, and tibial surface angle. The hindfoot alignment was evaluated using the varus-valgus angle (VVA) of the calcaneus. A visual analog scale (VAS) was used to evaluate ankle pain. The patient-reported outcome measure of the knee joint was the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score.

Results:

There were 110 patients in group 1 and 20 patients in group 2. In group 2, the change in talar inclination after MOWHTO was significantly greater and the changes in tibial plafond inclination, talar tilt, and VVA were significantly smaller compared with in group 1 (all P < .05). Ankle pain VAS scores were more severe in group 2 than in group 1 pre- and postoperatively (all P < .05), and group 2 reported that ankle pain worsened postoperatively ( P < .05). In both groups, knee WOMAC scores improved, and there were no differences between groups pre- or postoperatively (all P > .05). A multivariate regression analysis demonstrated that a small VVA change (odds ratio, 0.775; P = .027) and severe OA grade of the ankle joint preoperatively (Kellgren-Lawrence grades 2-4 vs 0 and 1; odds ratio, 4.241 [ P = .046]) predicted increased ankle pain VAS scores after MOWHTO.

Conclusion:

Although the patient-reported outcome measures for the knee joint improved irrespective of the presence of ankle OA, ankle pain worsened after MOWHTO in patients with ankle OA. Inadequate compensatory change in hindfoot alignment increased ankle pain in these patients.
Experimental and numerical investigation of core splicing configurations in an advanced composite sandwich structural system
Authors: Aqel Rawan , Severson Patrick , Elhajjar Rani
Published: January 2023

In this study, an investigation is presented on the effects of the honeycomb core splice gaps on the performance of advanced composite sandwich structures consisting of aluminum honeycomb core and carbon-fiber/epoxy facesheets. The experimental and numerical investigation explores the effects of core thickness, facesheet thickness, splice gap width, and the presence of core splice adhesive on the structural response of the composite sandwich structure. The specimens fabricated with the splices exposed to primarily bending and shear to understand further how the core splice interacts with the overall structural response under such loading conditions. In addition, 3D finite element models investigated the failure modes in the specimens by examining the strain fields for core-spliced sections of the sandwich structure under the experimentally observed loading conditions. Various failure modes are observed in the experiments ranging from core shear failure, facesheet debonding, or fracture depending on the location of the core splice, core, and the facesheet properties. The results illustrate the importance of proper detailing and analysis of core splices in composite structures made from sandwich designs.