bluuebunny/crossref_metadata_2025 huggingface dataset:
Chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances
Authors: Ke Li , Chen Xiasang , Dai Pingli , Liu Yong-Jun
Published: April 2023

The use of agricultural neonicotinoid insecticides has sub-lethal chronic effects on bees that are more prevalent than acute toxicity. Among these insecticides, thiacloprid, a commonly used compound with low toxicity, has attracted significant attention due to its potential impact on the olfactory and learning abilities of honeybees. The effect of sub-lethal larval exposure to thiacloprid on the antennal activity of adult honeybees (Apis mellifera L.) is not yet fully understood. To address this knowledge gap, laboratory-based experiments were conducted in which honeybee larvae were administered thiacloprid (0.5 mg/L and 1.0 mg/L). Using electroantennography (EAG), the impacts of thiacloprid exposure on the antennal selectivity to common floral volatiles were evaluated. Additionally, the effects of sub-lethal exposure on odor-related learning and memory were also assessed. The results of this study reveal, for the first time, that sub-lethal larval exposure to thiacloprid decreased honeybee antenna EAG responses to floral scents, leading to increased olfactory selectivity in the high-dose (1.0 mg/L) group compared to the control group (0 mg/L vs. 1.0 mg/L: p = 0.042). The results also suggest that thiacloprid negatively affected odor-associated paired learning acquisition, as well as medium-term (1 h) (0 mg/L vs. 1.0 mg/L: p = 0.019) and long-term memory (24 h) (0 mg/L vs. 1.0 mg/L: p = 0.037) in adult honeybees. EAG amplitudes were dramatically reduced following R-linalool paired olfactory training (0 mg/L vs. 1.0 mg/L: p = 0.001; 0 mg/L vs. 0.5 mg/L: p = 0.027), while antennal activities only differed significantly in the control between paired and unpaired groups. Our results indicated that exposure to sub-lethal concentrations of thiacloprid may affect olfactory perception and learning and memory behaviors in honeybees. These findings have important implications for the safe use of agrochemicals in the environment.
Comparative transcriptome and metabolite survey reveal key pathways involved in the control of the chilling injury disorder superficial scald in two apple cultivars, ‘Granny Smith’ and ‘Ladina’
Authors: Vittani Lorenzo , Populin Francesca , Stuerz Stefan , Buehlmann Andreas , Khomenko Iuliia , Biasioli Franco , Bühlmann-Schütz Simone , Vrhovsek Urska , Masuero Domenico , Zanella Angelo , Busatto Nicola , Costa Fabrizio
Published: April 2023

The low temperature normally applied to prevent fruit decay during the storage of apples, can also triggers the onset of a chilling injury disorder known as superficial scald. In this work, the etiology of this disorder and the mechanism of action of two preventing strategies, such as the application of 1-MCP (1-methylcyclopropene) and storage at low oxygen concentration in ‘Granny Smith’ and ‘Ladina’ apple cultivars were investigated. The metabolite assessment highlighted a reorganization of specific metabolites, in particular flavan-3-ols and unsaturated fatty acids, while the genome-wide transcriptomic analysis grouped the DEGs into four functional clusters. The KEGG pathway and GO enrichment analysis, together with the gene-metabolite interactome, showed that the treatment with 1-MCP prevented the development of superficial scald by actively promoting the production of unsaturated fatty acids, especially in ‘Granny Smith’. ‘Ladina’, more susceptible to superficial scald and less responsive to the preventing strategies, was instead characterized by a higher accumulation of very long chain fatty acids. Storage at low oxygen concentration stimulated a higher accumulation of ethanol and acetaldehyde together with the expression of genes involved in anaerobic respiration, such as malate, alcohol dehydrogenase and pyruvate decarboxylase in both cultivars. Low oxygen concentration, likewise 1-MCP, through a direct control on ethylene prevented the onset of superficial scald repressing the expression of PPO, a gene encoding for the polyphenol oxidase enzyme responsible of the oxidation of chlorogenic acid. Moreover, in ‘Granny Smith’ apple, the expression of three members of the VII subgroups of ERF genes, encoding for elements coordinating the acclimation process to hypoxia in plants was observed. The global RNA-Seq pattern also elucidated a specific transcriptomic signature between the two cultivars, disclosing the effect of the different genetic background in the control of this disorder.
Application of a novel strong promoter from Chinese fir (Cunninghamia lanceolate) in the CRISPR/Cas mediated genome editing of its protoplasts and transgenesis of rice and poplar
Authors: Ye Shanwen , Ding Wensha , Bai Weiyuan , Lu Jiaao , Zhou Linying , Ma Xiangqing , Zhu Qiang
Published: April 2023

Novel constitutive promoters are essential for plant biotechnology. Although in angiosperms, a number of promoters were applied in monocots or dicots genetic engineering, only a few promoters were used in gymnosperm. Here we identified two strong promoters (Cula11 and Cula08) from Chinese fir (C. lanceolate) by screening the transcriptomic data and preliminary promoter activity assays in tobacco. By using the newly established Chinese fir protoplast transient expression technology that enables in vivo molecular biology studies in its homologous system, we compared the activities of Cula11 and Cula08 with that of the commonly used promoters in genetic engineering of monocots or dicots, such as CaM35S, CmYLCV, and ZmUbi, and our results revealed that Cula11 and Cula08 promoters have stronger activities in Chinese fir protoplasts. Furthermore, the vector containing Cas gene driven by Cula11 promoter and sgRNA driven by the newly isolated CulaU6b polyIII promoters were introduced into Chinese fir protoplasts, and CRISPR/Cas mediated gene knock-out event was successfully achieved. More importantly, compared with the commonly used promoters in the genetic engineering in angiosperms, Cula11 promoter has much stronger activity than CaM35S promoter in transgenic poplar, and ZmUbi promoter in transgenic rice, respectively, indicating its potential application in poplar and rice genetic engineering. Overall, the novel putative constitutive gene promoters reported here will have great potential application in gymnosperm and angiosperm biotechnology, and the transient gene expression system established here will serve as a useful tool for the molecular and genetic analyses of Chinese fir genes.
Alexithymia and sensory processing sensitivity account for unique variance in the prediction of emotional contagion and empathy
Authors: McQuarrie Amanda M. , Smith Stephen D. , Jakobson Lorna S.
Published: April 2023

Introduction

Empathy—the ability to identify and share another person’s emotional state—is an important socio-emotional process arising, in part, from emotional contagion. In the current study, we assessed unique variance in emotional contagion and other empathy-related constructs accounted for by two personality traits, alexithymia and sensory processing sensitivity (SPS), when controlling for childhood emotional abuse and current depressed mood.

Methods

A sample of 305 adults (Mage = 20.1 years) watched brief film clips chosen to induce various emotional states. After each film, the participants rated how strongly they experienced each of nine different emotions. They then completed self-report measures of alexithymia, SPS, empathy-related constructs, childhood emotional abuse, and current mood.

Results

Those scoring high (vs. low) on SPS reported stronger primary emotions and a larger range of emotions when watching the films and were more apt to believe that their emotions matched those of the individuals featured in the films. They also scored higher on both self-oriented processes (such as the tendency to feel personal distress in tense situations) and other-oriented processes (such as perspective taking and empathic concern) related to empathy. Individuals scoring high (vs. low) on alexithymia reported feeling a larger range of emotions while watching the films but scored lower on other-oriented processes related to empathy. After controlling for SPS and alexithymia, current depressed mood predicted experiencing less varied reactions to mixed valence films that elicited strong feelings of embarrassment/humiliation, and less amusement when watching positive films. Childhood emotional abuse did not emerge as a predictor of emotional contagion or empathy.

Discussion

We propose that the strong and nuanced feelings elicited in those scoring high on SPS by observing others support their personal view that they are highly empathic. In contrast, by failing to closely examine their own mixed reactions to others, individuals with alexithymia may find it difficult to connect with, understand, and respond to others’ feelings.
Clinician perception of pathological narcissism in females: a vignette-based study
Authors: Green Ava , MacLean Rory , Charles Kathy
Published: April 2023

The DSM-5 reports that up to 75% of those diagnosed with Narcissistic Personality Disorder (NPD) are males, which denotes that narcissism is a clinical phenomenon that operates differently in men and women. Vulnerable narcissism, which tends to be more prevalent in females and is currently under-appreciated in the DSM-5, may be diagnosed as other “vulnerable” disorders (e.g., Borderline Personality Disorder; BPD). The current study investigated gender differences in clinicians’ perceptions of narcissistic pathology. Adopting an online vignette-based study, clinicians (N = 108; 79 females) read clinical case vignettes of hypothetical patients and provided diagnostic ratings of existing personality disorders. Clinicians’ diagnostic ratings of NPD were concurrent with the vignette containing grandiose narcissism symptoms, irrespective of patient gender. However, when presented with a vulnerable narcissism vignette, clinicians were significantly more likely to attribute a BPD diagnosis in female patients, compared to male patients. Clinicians with a psychodynamic approach and more experience in practice were also more likely to label vulnerable narcissism symptoms as NPD, compared to those with a CBT approach and less experience in practice. The clinical implications of these results support the shift toward assessing personality dysfunction based on dimensional trait domains.
The varying social dynamics in orally transmitted and notated vs. improvised musical performance
Authors: Hadar Tamar , Rabinowitch Tal-Chen
Published: April 2023

Musical performance can be viewed as an intricate form of social behavior. Accordingly, the rich diversity of existing musical styles and traditions may reflect distinct modes of social interaction. To gain a better understanding of the relations between musical style and social dynamics, we have formulated a framework for dissecting different genres of musical performance according to key social criteria. In particular, we contemplate on the continuum ranging from strictly orally transmitted and notated to fully improvised music, and its relation to general compliance with social norms and structure, borrowing key concepts from tight-loose theory, a powerful paradigm for studying societal behaviors and tendencies. We apply this approach to analyze four distinct prominent musical genres, providing a detailed mapping between musical style and social dynamics. This work highlights important factors that link between musical performance and social interaction, and will enable future experimental unraveling of social aspects of musical performance as expressed by different musical styles and practices.
An experimental study using a sexual strategies explanation to reduce homophobia toward gay men among lay people and healthcare professionals in China
Authors: Gao Qianhui , Antfolk Jan , Santtila Pekka
Published: April 2023

Introduction

Homophobic discrimination and stigmatization, especially from healthcare professionals, are important stressors for gay men. Homophobia may be partly rooted in seeing some gay men having casual sex and many sexual partners as a signal of mental problems. Sexual Strategies Theory (SST) suggests that such sexual behavior is a result of different sexual strategies men and women tend to adopt and is unrelated to sexual orientationper se. This study aimed to investigate (1) the effectiveness of providing an SST explanation for gay men’s sexual behavior in reducing homophobia among both lay persons and healthcare professionals; (2) differences in homophobia between healthcare professionals and lay people and also between medical and non-medical professionals.

Methods

The main analyses included 492 heterosexual participants recruited online via Chinese social media and sample services in 2021. Of these, 227 were healthcare professionals (128 medical, 99 non-medical) and 265 were lay people. The participants were randomly assigned into an experimental group given the SST explanation (n= 126), an active control group given a Minority Stress (MS) explanation (n= 184), and a control group (n= 182). After the manipulation, homophobia, knowledge about homosexuality, professional homophobic attitude, gay affirmative practice, and contact with gay men were assessed.

Results

The results of factor analysis suggested dividing homophobia into Oppressing Homophobia (Oppressing HP) describing believing that gay men should have fewer rights and Pathologizing Homophobia (Pathologizing HP) describing believing that the sexual behavior of gay men is a signal of mental problem. Importantly, the SST explanation reduced Pathologizing HP while the MS explanation reduced Oppressing HP. Healthcare professionals reported more Oppressing HP than lay people, and medical professionals conducted less gay affirmative practice than non-medical professionals.

Conclusion

An SST explanation can potentially reduce some aspects of homophobia among both healthcare professionals and lay people. Also, worryingly, Chinese healthcare professionals, especially medical professionals, reported more homophobia than lay individuals.
Investigating the effects of autonomy-supportive parenting practices on Italian young adolescent students’ motivation to defend victims of bullying: findings on the mediating roles of reactance, depression, anxiety, and stress
Authors: Iotti Nathaniel Oliver , Menin Damiano , Longobardi Claudio , Jungert Tomas
Published: April 2023

This study investigated the effect of autonomy-supportive parenting practices on young adolescents’ self-reported motivation to defend victims of bullying, and the possible mediating effects of factors such as reactance, anxiety, depression, and stress.

Methods

Data were collected from 578 Italian public school students ages 10–14 (Mage= 11.8 years, 52% boys), who completed a survey in their classroom. The survey included self-report measures of parental orientation, motivation to defend victims of bullying, reactance, anxiety, depression, and stress.

Results

We found that autonomy-supportive parenting had a positive effect on autonomous motivation to defend, and that this effect was weakly mediated by reactance. Moreover, autonomy-supportive parenting had a negative effect on extrinsic motivation to defend, which was partially mediated by reactance. Reactance had a positive direct effect on extrinsic motivation to defend, but results also showed that anxiety, depression, and stress did not mediate the effect of autonomy-supportive parenting on motivation to defend. Additionally, autonomy-supportive parenting appeared to play a protective role, being associated with lower levels of reactance, anxiety, depression, and stress. Finally, gender differences were found in our sample, with extrinsic motivation to defend being more prevalent in boys, and autonomous motivation to defend being more prevalent in girls. Girls also reported higher levels of anxiety, depression, and stress, compared to boys.

Conclusion

Our findings show that autonomy-supportive parenting practices play a significant role in fostering young adolescents’ motivation to defend victims of bullying, and that they are also linked with lower feelings of reactance, anxiety, depression, and stress. We argue that interventions aimed at contrasting bullying and cyberbullying among youths should seek to involve parents more and promote the adoption of more autonomy-supportive parenting practices, due to their consistently proven beneficial effects.
Teachers’ contributions to the school climate and using empathy at work: implications from qualitative research in two European countries
Authors: Martinsone Baiba , Žydžiūnaite Vilma
Published: April 2023

This research aimed to reveal the factors that, based on their experiences, teachers consider important in their contribution to a positive school climate and in their relationship with students when it comes to using empathy. Teachers from two European countries—Latvia (n = 83) and Lithuania (n = 69)—participated in the research. Data were collected through focus group-based interviews and analyzed using latent qualitative content analysis. The results revealed the teachers’ shared understanding of the importance of communication and professional development. However, only Lithuanian teachers emphasized strengthening institutional values and other more collectivistic aspects; the answers of Latvian teachers were specific and suggested more of an individualistic attitude, like allocating personal responsibility to use appropriate instructional strategies. This study draws attention to contextual and cultural factors of teachers’ work and actualizes their educational needs.
Patterns of risk—Using machine learning and structural neuroimaging to identify pedophilic offenders
Authors: Popovic David , Wertz Maximilian , Geisler Carolin , Kaufmann Joern , Lähteenvuo Markku , Lieslehto Johannes , Witzel Joachim , Bogerts Bernhard , Walter Martin , Falkai Peter , Koutsouleris Nikolaos , Schiltz Kolja
Published: April 2023

Background

Child sexual abuse (CSA) has become a focal point for lawmakers, law enforcement, and mental health professionals. With high prevalence rates around the world and far-reaching, often chronic, individual, and societal implications, CSA and its leading risk factor, pedophilia, have been well investigated. This has led to a wide range of clinical tools and actuarial instruments for diagnosis and risk assessment regarding CSA. However, the neurobiological underpinnings of pedosexual behavior, specifically regarding hands-on pedophilic offenders (PO), remain elusive. Such biomarkers for PO individuals could potentially improve the early detection of high-risk PO individuals and enhance efforts to prevent future CSA.

Aim

To use machine learning and MRI data to identify PO individuals.

Methods

From a single-center male cohort of 14 PO individuals and 15 matched healthy control (HC) individuals, we acquired diffusion tensor imaging data (anisotropy, diffusivity, and fiber tracking) in literature-based regions of interest (prefrontal cortex, anterior cingulate cortex, amygdala, and corpus callosum). We trained a linear support vector machine to discriminate between PO and HC individuals using these WM microstructure data.Post hoc, we investigated the PO model decision scores with respect to sociodemographic (age, education, and IQ) and forensic characteristics (psychopathy, sexual deviance, and future risk of sexual violence) in the PO subpopulation. We assessed model specificity in an external cohort of 53 HC individuals.

Results

The classifier discriminated PO from HC individuals with a balanced accuracy of 75.5% (sensitivity = 64.3%, specificity = 86.7%,P5000= 0.018) and an out-of-sample specificity to correctly identify HC individuals of 94.3%. The predictive brain pattern contained bilateral fractional anisotropy in the anterior cingulate cortex, diffusivity in the left amygdala, and structural prefrontal cortex-amygdala connectivity in both hemispheres. This brain pattern was associated with the number of previous child victims, the current stance on sexuality, and the professionally assessed risk of future sexual violent reoffending.

Conclusion

Aberrant white matter microstructure in the prefronto-temporo-limbic circuit could be a potential neurobiological correlate for PO individuals at high-risk of reoffending with CSA. Although preliminary and exploratory at this point, our findings highlight the general potential of MRI-based biomarkers and particularly WM microstructure patterns for future CSA risk assessment and preventive efforts.
Causal effects of opioids on postpartum depression: a bidirectional, two-sample Mendelian randomization study
Authors: Jiang Yage , Wei Donglei , Xie Yubo
Published: April 2023

Background

Postpartum depression is the most common psychiatric disorder in pregnant women during the postpartum period and requires early detection and treatment. Previous studies have found that opioids use affects depression and anxiety disorders. Although it has long been suspected that opioids may contribute to the development of postpartum depression, observational studies are susceptible to confounding factors and reverse causality, making it difficult to determine the direction of these associations.

Methods

To examine the causal associations between opioids and non-opioid analgesics with postpartum depression, we utilized large-scale genome-wide association study (GWAS) genetic pooled data from two major databases: opioids, salicylate analgesic, non-steroidal anti-inflammatory drugs (NSAIDs), and aniline analgesics GWAS data from the United Kingdom Biobank database. GWAS data for postpartum depression were obtained from the FinnGen database. The causal analysis methods used random-effects inverse variance weighting (IVW), and complementary sensitivity analyses using weighted median, MR-Egger method, and MR-PRESSO test.

Results

In the IVW analysis, Mendelian randomization (MR) analysis showed that opioids increased the risk of postpartum depression (OR, 1.169; 95% CI, 1.050–1.303; p = 0.005). Bidirectional analysis showed a significant causal relationship between genetically predicted postpartum depression and increased risk of opioids and non-opioid analgesics use (opioids OR, 1.118; 95% CI, 1.039–1.203; p = 0.002; NSAIDs OR, 1.071; 95% CI, 1.022–1.121; p = 0.004; salicylates OR, 1.085; 95% CI, 1.026–1.146; p = 0.004; and anilides OR, 1.064; 95% CI, 1.018–1.112; p = 0.006). There was no significant heterogeneity or any significant horizontal pleiotropy bias in the sensitivity analysis.

Conclusion

Our study suggests a potential causal relationship between opioids use and the risk of postpartum depression. Additionally, postpartum depression is associated with an increased risk of opioids and non-opioid analgesics use. These findings may provide new insights into prevention and intervention strategies for opioids abuse and postpartum depression.
Agomelatine in the treatment of anhedonia, somatic symptoms, and sexual dysfunction in major depressive disorder
Authors: Huang Juan , Xie Xiao-Meng , Lyu Nan , Fu Bing-Bing , Zhao Qian , Zhang Ling , Wang Gang
Published: April 2023

Objective

This study evaluated the treatment outcomes of agomelatine on anhedonic state, anxiety/somatic symptoms, and sexual function in Chinese patients with major depressive disorder (MDD).

Method

In total, 93 adult patients with MDD were enrolled, and 68 of them were included in a prospective, open-label, multicenter clinical study. All patients received agomelatine monotherapy during a 9-week treatment phase. The effectiveness of the treatment was reflected by the improvement of anhedonia and somatic symptoms based on the 17-item Hamilton Depression Rating Scale (HAMD-17). In addition, the Arizona Sexual Dysfunction Scale (ASEX), Sheehan Disability Scale (SDS), and Short Form of Quality-of-Life Enjoyment and Satisfaction Questionnaire (Q-LES-Q-SF) were administered to all participants at baseline and at the 3-, 6-, and 9-week follow-ups.

Results

After 9 weeks of treatment with agomelatine, the response and remission rates were 73.5% and 39.7%, respectively. Somatic symptoms significantly improved at week 9 (p < 0.001), and significant effects were also observed on the HAMD anhedonia items (p < 0.001). The patients exhibited lower levels of disease severity (the SDS score dropped from 15.52 ± 4.7 to 7.09 ± 5.62 at week 9; the ASEX score dropped from 21.89 ± 4.06 to 16.19 ± 4.79, p < 0.001) and higher levels of QOL (the Q-LES-Q-SF score dropped from 41.02 ± 5.99 to 50.49 ± 8.57, p < 0.001) during the follow-up. Furthermore, treatment with agomelatine improved depressive symptoms without causing serious adverse events.

Conclusion

These analyses indicate that agomelatine is a treatment option for improving anhedonic status, anxiety/somatic symptoms, and sexual dysfunction in MDD patients.
Operational definition of precipitated opioid withdrawal
Authors: Dunn Kelly E. , Bird H. Elizabeth , Bergeria Cecilia L. , Ware Orrin D. , Strain Eric C. , Huhn Andrew S.
Published: April 2023

Background

Opioid withdrawal can be expressed as both a spontaneous and precipitated syndrome. Although spontaneous withdrawal is well-characterized, there is no operational definition of precipitated opioid withdrawal.

Methods

People (N = 106) with opioid use disorder maintained on morphine received 0.4 mg intramuscular naloxone and completed self-report (Subjective Opiate Withdrawal Scale, SOWS), visual analog scale (VAS), Bad Effects and Sick, and observer ratings (Clinical Opiate Withdrawal Scale, COWS). Time to peak severity and minimal clinically important difference (MCID) in withdrawal severity were calculated. Principal component analysis (PCA) during peak severity were conducted and analyzed with repeated measures analyses of variance (ANOVA).

Results

Within 60 min, 89% of people reported peak SOWS ratings and 90% of people had peak COWS scores as made by raters. Self-reported signs of eyes tearing, yawning, nose running, perspiring, hot flashes, and observed changes in pupil diameter and rhinorrhea/lacrimation were uniquely associated with precipitated withdrawal. VAS ratings of Bad Effect and Sick served as statistically significant severity categories (0, 1–40, 41–80, and 81–100) for MCID evaluations and revealed participants' identification with an increase of 10 [SOWS; 15% maximum percent effect (MPE)] and 6 (COWS; 12% MPE) points as meaningful shifts in withdrawal severity indicative of precipitated withdrawal.

Conclusion

Data suggested that a change of 10 (15% MPE) and 6 (12% MPE) points on the SOWS and COWS, respectively, that occurred within 60 min of antagonist administration was identified by participants as a clinically meaningful increase in symptom severity. These data provide a method to begin examining precipitated opioid withdrawal.
Epidemiology and serotype distribution of Streptococcus pneumoniae carriage among influenza-like illness cases in metropolitan Vientiane, Lao PDR: a community-based cohort study
Authors: Sanchez Picot Valentina , Keovichith Inthalaphone , Paboriboune Phimpha , Flaissier Bruno , Saadatian-Elahi Mitra , Rudge James W.
Published: April 2023

Background

Data on the epidemiology of Streptococcus pneumoniae among influenza-like illness (ILI) cases, particularly in low- and middle-income countries are scarce. This study assessed the prevalence, risk factors and serotype distribution of S. pneumoniae carriage among ILI cases in metropolitan Vientiane, Lao People's Democratic Republic. The 13-valent pneumococcal conjugate vaccine (PCV13) was introduced among infants in October 2013.

Methods

Active ILI surveillance was conducted through weekly phone calls in an open community-based cohort study (April 2015–February 2019), involving 5,690 participants from 1,142 randomly selected households. Participants reporting ILI symptoms provided a nasopharyngeal swab and answered a questionnaire. S. pneumoniae and serotype pneumococcal-positive samples were screened by Multiplex PCR assays. Chi-squared tests and generalized linear mixed models were used to test for variables associated with pneumococcal positivity.

Results

Among 1,621 ILI episodes, 269 (16.6%) tested positive for nasopharyngeal pneumococcal carriage, with the highest prevalence (55.4%) in children under 5 years. Pneumococcal carriage was significantly associated with concurrent detection of Hemophilus influenzae (adjusted odds ratio [aOR]: 6.93; 95% CI: 2.10–22.9) and exposure to household cigarette smoke (aOR: 1.65; 95% CI: 1.07–2.54). PCV13 serotypes accounted for 37.8% of all pneumococcal isolates. Detection of PCV13 serotypes among ILI cases aged under 5 years declined significantly between 2015/16 and 2018/19.

Conclusions

Community-based surveillance of S. pneumoniae among ILI cases complement surveillance at healthcare facilities to provide a more complete picture of pneumococcal carriage. Our findings contribute also to the growing body of evidence on the effects of PCV13 introduction on circulating serotypes and their potential replacement.
HPV-related oropharyngeal cancer early detection in gay and bisexual men is an “orphan” practice: A qualitative analysis among healthcare providers
Authors: Zoschke I. Niles , Bennis Sarah L. , Wilkerson J. Michael , Stull Cynthia L. , Nyitray Alan G. , Khariwala Samir S. , Nichols C. Mark , Rosser B. R. Simon , Flash Charlene , Ross Michael W.
Published: April 2023

Introduction

Among US men, oropharyngeal cancer (cancer of the back of the mouth and throat) is the 8th most common cancer. If detected early, human papillomavirus (HPV)-16-associated oropharyngeal cancer has a high 5-year survival rate. Risk factors such as high numbers of oral sex partners, disparities in smoking and drinking, and low rates of HPV vaccination may put gay and bisexual men at even higher risk for oropharyngeal cancer.

Methods

We recruited 21 healthcare providers in Minneapolis-St. Paul, Minnesota and Houston, Texas to participate in semi-structured interviews. Nurses, physician assistants, dental hygienists, and dentists were asked about their clinical experiences serving gay and bisexual men and opinions on potential interventions for the early detection of oropharyngeal cancer.

Results

Providers typically did not tailor health screenings and examinations for gay and bisexual men. Participants lacked confidence in their ability to effectively implement routine screening for oropharyngeal cancer. The extent to which oropharyngeal cancer screening was incorporated into clinical practice varied by specialty, and practices necessary to detect it were scattered across clinical environments. HIV- and LGBTQ-focused healthcare providers were more aware of HPV-associated oropharyngeal cancer in gay and bisexual men, and appeared readier to act and lead on this issue.

Discussion

Further studies should (1) evaluate protocols for oropharyngeal cancer detection; (2) identify and assess the acceptability of screening in the community; and (3) study how to best close gaps in health services for gay and bisexual men which might contribute to low early detection rates of oropharyngeal cancer.
Study related factors associated with study engagement and student burnout among German university students
Authors: Olson Nils , Oberhoffer-Fritz Renate , Reiner Barbara , Schulz Thorsten
Published: April 2023

Introduction

Student burnout has become a health concern in higher education systems. Its prevalence rates are high due to specific demands in this life situation. It leads not only to increased academic dropout rates but is also associated with negative health outcomes both physically and mentally. Its counterpart is study engagement, which is a positive, fulfilling, study-related attitude characterized by energy, dedication, and absorption. There has not been a systematical approach covering the demands directly posed by the academic environment itself. Additionally, academic subject fields apart from medicine and nursing sciences have been mostly neglected in regards to this research field. The aim of the study is therefore to identify contributing factors for both burnout and engagement within the academic environment in a sample of different subject fields at a German university.

Methods

In a cross-sectional study, a sample of 3,451 students of all academic subjects at a technical university in Germany has been analyzed using an online survey. Sociodemographic data, study engagement, student burnout, study satisfaction, academic workload, number of semesters and occupational liabilities have been analyzed. Binary logistic regression was used to determine the associations of burnout symptoms and study engagement.

Results

Almost a third of the students showed frequent burnout symptoms, while 42.5% showed a high degree of study engagement with no differences in gender. Age was identified as a risk factor for frequent signs of cynicism (OR = 1.073). Study satisfaction (OR between 0.459 and 0.702), semester progression (OR = 0.959) and working moderately (OR between 0.605 and 0.637) was associated with fewer symptoms in different burnout-dimensions. Study satisfaction is positively associated with study engagement (OR = 2.676). Academic workload is positively related to both burnout (OR between 1.014 and 1.021) and study engagement (OR = 1.014).

Discussion

A substantial number of students show frequent symptoms of burnout and the majority is not highly engaged. The included factors contribute to the model to various degrees and show that university-bound factors play a major role. Fostering a supportive environment is key for study engagement, health and well-being. The inclusion of further, individual factors should be a future concern in order to find and promote strategies for a healthy education system.
Monitoring of indoor bioaerosol for the detection of SARS-CoV-2 in different hospital settings
Authors: Tedeschini Emma , Pasqualini Stefania , Emiliani Carla , Marini Ettore , Valecchi Alessandro , Laoreti Chiara , Ministrini Stefano , Camilloni Barbara , Castronari Roberto , Patoia Lucio , Merante Francesco , Baglioni Stefano , De Robertis Edoardo , Pirro Matteo , Mencacci Antonella , Pasqualini Leonella
Published: April 2023

Background

Spore Trap is an environmental detection technology, already used in the field of allergology to monitor the presence and composition of potentially inspirable airborne micronic bioparticulate. This device is potentially suitable for environmental monitoring of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in hospital, as well as in other high-risk closed environments. The aim of the present study is to investigate the accuracy of the Spore Trap system in detecting SARS-CoV-2 in indoor bioaerosol of hospital rooms.

Methods

The Spore Trap was placed in hospital rooms hosting patients with documented SARS-CoV-2 infection (n = 36) or, as a negative control, in rooms where patients with documented negativity to a Real-Time Polymerase Chain Reaction molecular test for SARS-CoV-2 were admitted (n = 10). The monitoring of the bioaerosol was carried on for 24 h. Collected samples were analyzed by real-time polymerase chain reaction.

Results

The estimated sensitivity of the Spore Trap device for detecting SARS-CoV-2 in an indoor environment is 69.4% (95% C.I. 54.3-84.4%), with a specificity of 100%.

Conclusion

The Spore Trap technology is effective in detecting airborne SARS-CoV-2 virus with excellent specificity and high sensitivity, when compared to previous reports. The SARS-CoV-2 pandemic scenario has suggested that indoor air quality control will be a priority in future public health management and will certainly need to include an environmental bio-investigation protocol.
The fate of SARS-CoV-2 viral RNA in coastal New England wastewater treatment plants
Authors: Aghababaei Mina , Colosimo Fabrizio , Malley James P. , Mouser Paula J.
Published: April 2023

Municipal sewage carries SARS-CoV-2 viruses shed in the human stool by infected individuals to wastewater treatment plants (WWTPs). It is well-established that increasing prevalence of COVID-19 in a community increases the viral load in its WWTPs. Despite the fact that wastewater treatment facilities serve a critical role in protecting downstream human and environmental health through removal or inactivation of the virus, little is known about the fate of the virus along the treatment train. To assess the efficacy of differing WWTP size and treatment processes in viral RNA removal we quantified two SARS-CoV-2 nucleocapsid (N) biomarkers (N1 and N2) in both liquid and solids phases for multiple treatment train locations from seven coastal New England WWTPs. SARS-CoV-2 biomarkers were commonly detected in the influent, primary treated, and sludge samples (returned activated sludge, waste activated sludge, and digested sludge), and not detected after secondary clarification processes or disinfection. Solid fractions had 470 to 3,700-fold higher concentrations of viral biomarkers than liquid fractions, suggesting considerably higher affinity of the virus for the solid phase. Our findings indicate that a variety of wastewater treatment designs are efficient at achieving high removal of SARS CoV-2 from effluent; however, quantifiable viral RNA was commonly detected in wastewater solids at various points in the facility. This study supports the important role municipal wastewater treatment facilities serve in reducing the discharge of SARS-CoV-2 viral fragments to the environment and highlights the need to better understand the fate of this virus in wastewater solids.
Multiple meanings of “equitable food systems”: food systems and discursive politics of change
Authors: Juskaite Greta , Haug Ruth
Published: April 2023

Calls to change or transform food systems have come to be widespread in recent years. With the recognition that current food systems are not only unsustainable but widely inequitable, these calls are increasingly articulated in terms of the need to craft “equitable food systems.” The purpose of this study is to question how “equitable food systems” are given meaning in ongoing discourses that shape the direction of food systems change. Finding the best strategies for food systems change is a subject of intense debate, making it an inherently political affair. In this study, our strategy for knowing about these politics is the analysis of discourses through which they are expressed. Through a literature review, this article identifies four discourses driving food systems change: (a) productionism, (b) redistributionism, (c) anti-capitalism, and (d) AID: donor rescue. Drawing on insights from existing literature, the study explores these discourses in relation to equity concerns. The analysis reveals that multiple meanings of “equitable food systems” exist, hinging on varied ideas about inequity, change, and the essence of human wellbeing. Materializing into practical strategies to progress food systems change, the multiplicity of meanings implies inevitable trade-offs when one is prioritized over the other. As such, through our analysis, we contend the need for more democracy when negotiating policy directions forward. All four discourses might have some merit that could become advantageous in finding contextually appropriate pathways toward more equitable food systems. However, corporate voices and perspectives tend to be louder than those of producers, workers, and consumers, reflecting and reproducing power imbalance within policy negotiations and the global society more broadly. Bridging such a power divide is thus essential to balance out food systems change discourses to allow for conductive combinations of elements from each to be created to anchor pursuits in food systems change that truly foster more equitable ways going forward.
Acetabular rim extension using a personalized titanium implant for treatment of hip dysplasia in dogs: short-term results
Authors: Kwananocha Irin , Magré Joëll , Willemsen Koen , Weinans Harrie , Sakkers Ralph J. B. , How Thijs , Verseijden Femke , Tryfonidou Marianna A. , van der Wal Bart C. H. , Meij Björn P.
Published: April 2023

Hip dysplasia (HD) is a common orthopedic problem in young dogs. To decrease the laxity of the hip joint related to HD, the surgical treatments are recommended to increase femoral head coverage. ACEtabular rim eXtension (ACE-X) using a personalized 3-dimensional printed titanium shelf implant is a new surgical treatment to increase femoral head coverage and decrease laxity of the dysplastic hip joint, however, the efficacy is less know. Client-owned dogs older than 6 months with clinical signs of coxofemoral joint subluxation and radiographic evidence of HD with no or mild osteoarthritis (OA) were included. The Norberg angle (NA), linear percentage of femoral head overlap (LFO), and percentage of femoral head coverage (PC) were investigated radiographically and with computed tomography (CT) before and after surgery. OA was graded (scores 0–3) according to the maximum osteophyte size measured on CT. In addition, joint laxity (Ortolani) test results, gait analysis, and the Helsinki chronic pain index (HCPI) questionnaire were obtained at preoperative, immediately postoperative and at 1.5- and 3-month evaluations. Acetabular rim extension was performed in 61 hips of 34 dogs; NA, LFO, and PC were significantly higher immediately postoperatively and at the 1.5- and 3-month follow-up examinations compared with preoperative values (p< 0.05). Osteophyte size gradually increased over time (p< 0.05). The OA score significantly increased between preoperatively and directly postoperatively, and between preoperatively and at 3-month follow-up (p< 0.05). The laxity test normalized in 59 out of 61 hips after surgery, and the HCPI questionnaire showed that the pain score decreased significantly at 1.5 and 3 months, postoperatively. The force plate showed no significant improvement during the 3 months follow-up. Although pain reduction by the implant was unclear in short-term results, a personalized shelf implant significantly increased femoral head coverage and eliminated subluxation of the dysplastic hip joint. Further studies are required to study the long-term efficacy of gait, chronic pain, and progression of osteoarthritis.
France’s New Lung Transplant Allocation System: Combining Equity With Proximity by Optimizing Geographic Boundaries Through the Supply/Demand Ratio
Authors: Bayer Florian , Dorent Richard , Cantrelle Christelle , Legeai Camille , Kerbaul François , Jacquelinet Christian
Published: April 2023

A new lung allocation system was introduced in France in September 2020. It aimed to reduce geographic disparities in lung allocation while maintaining proximity. In the previous two-tiered priority-based system, grafts not allocated through national high-urgency status were offered to transplant centres according to geographic criteria. Between 2013 and 2018, significant geographic disparities in transplant allocation were observed across transplant centres with a mean number of grafts offered per candidate ranging from 1.4 to 5.2. The new system redistricted the local allocation units according to supply/demand ratio, removed regional sharing and increased national sharing. The supply/demand ratio was defined as the ratio of lungs recovered within the local allocation unit to transplants performed in the centre. A driving time between the procurement and transplant centres of less than 2 h was retained for proximity. Using a brute-force algorithm, we designed new local allocation units that gave a supply/demand ratio of 0.5 for all the transplant centres. Under the new system, standard-deviation of graft offers per candidate decreased from 0.9 to 0.5 (p = 0.08) whereas the mean distance from procurement to transplant centre did not change. These preliminary results show that a supply/demand ratio-based allocation system can achieve equity while maintaining proximity.
Ising-Based Kernel Clustering
Authors: Kumagai Masahito , Komatsu Kazuhiko , Sato Masayuki , Kobayashi Hiroaki
Published: April 2023

Combinatorial clustering based on the Ising model is drawing attention as a high-quality clustering method. However, conventional Ising-based clustering methods using the Euclidean distance cannot handle irregular data. To overcome this problem, this paper proposes an Ising-based kernel clustering method. The kernel clustering method is designed based on two critical ideas. One is to perform clustering of irregular data by mapping the data onto a high-dimensional feature space by using a kernel trick. The other is the utilization of matrix–matrix calculations in the numerical libraries to accelerate preprocess for annealing. While the conventional Ising-based clustering is not designed to accept the transformed data by the kernel trick, this paper extends the availability of Ising-based clustering to process a distance matrix defined in high-dimensional data space. The proposed method can handle the Gram matrix determined by the kernel method as a high-dimensional distance matrix to handle irregular data. By comparing the proposed Ising-based kernel clustering method with the conventional Euclidean distance-based combinatorial clustering, it is clarified that the quality of the clustering results of the proposed method for irregular data is significantly better than that of the conventional method. Furthermore, the preprocess for annealing by the proposed method using numerical libraries is by a factor of up to 12.4 million × from the conventional naive python’s implementation. Comparisons between Ising-based kernel clustering and kernel K-means reveal that the proposed method has the potential to obtain higher-quality clustering results than the kernel K-means as a representative of the state-of-the-art kernel clustering methods.
Learning Pose Dynamical System for Contact Tasks under Human Interaction
Authors: Yang Shangshang , Gao Xiao , Feng Zhao , Xiao Xiaohui
Published: April 2023

Robots are expected to execute various operation tasks like a human by learning human working skills, especially for complex contact tasks. Increasing demands for human–robot interaction during task execution makes robot motion planning and control a considerable challenge, not only to reproduce demonstration motion and force in the contact space but also to resume working after interacting with a human without re-planning motion. In this article, we propose a novel framework based on a time-invariant dynamical system (DS), taking into account both human skills transfer and human–robot interaction. In the proposed framework, the human demonstration trajectory was modeled by the pose diffeomorphic DS to achieve online motion planning. Furthermore, the motion of the DS was modified by admittance control to satisfy different demands. We evaluated the method with a UR5e robot in the contact task of the composite woven layup. The experimental results show that our approach can effectively reproduce the trajectory and force learned from human demonstration, allow human–robot interaction safely during the task, and control the robot to return to work automatically after human interaction.