bluuebunny/crossref_metadata_2025 huggingface dataset:
Improved estimates for the linear Molodensky problem
Authors: Sansò Fernando , Betti Barbara
Published: May 2024

The paper deals with the linearized Molodensky problem, when data are supposed to be square integrable on the telluroidS, proving that a solution exists, is unique and is stable in a space of harmonic functions with square integrable gradient onS. A similar theorem has already been proved by Sansò and Venuti (J Geod 82:909–916, 2008). Yet the result basically requires thatSshould have an inclination of less than$$60^\circ $$60∘with respect to the vertical, or better to the radial direction. This constraint could result in a severe regularization for the telluroid specially in mountainous areas. The paper revises the result in an effort to improve the above estimates, essentially showing that the inclination ofScould go up to$$75^\circ $$75∘. At the same time, the proof is made precise mathematically and hopefully more readable in the geodetic community.
Extracellular vesicles from Aggregatibacter actinomycetemcomitans exhibit potential antitumorigenic effects in oral cancer: a comparative in vitro study
Authors: Metsäniitty Marjut , Hasnat Shrabon , Öhman Carina , Salo Tuula , Eklund Kari K. , Oscarsson Jan , Salem Abdelhakim
Published: May 2024

Aggregatibacter actinomycetemcomitans is an opportunistic Gram-negative periodontopathogen strongly associated with periodontitis and infective endocarditis. Recent evidence suggests that periodontopathogens can influence the initiation and progression of oral squamous cell carcinoma (OSCC). Herein we aimed to investigate the effect of A. actinomycetemcomitans-derived extracellular vesicles (EVs) on OSCC cell behavior compared with EVs from periodontopathogens known to associate with carcinogenesis. EVs were isolated from: A. actinomycetemcomitans and its mutant strains lacking the cytolethal distending toxin (CDT) or lipopolysaccharide (LPS) O-antigen; Porphyromonas gingivalis; Fusobacterium nucleatum; and Parvimonas micra. The effect of EVs on primary and metastatic OSCC cells was assessed using cell proliferation, apoptosis, migration, invasion, and tubulogenesis assays. A. actinomycetemcomitans-derived EVs reduced the metastatic cancer cell proliferation, invasion, tubulogenesis, and increased apoptosis, mostly in CDT- and LPS O-antigen-dependent manner. EVs from F. nucleatum impaired the metastatic cancer cell proliferation and induced the apoptosis rates in all OSCC cell lines. EVs enhanced cancer cell migration regardless of bacterial species. In sum, this is the first study demonstrating the influence of A. actinomycetemcomitans-derived EVs on oral cancer in comparison with other periodontopathogens. Our findings revealed a potential antitumorigenic effect of these EVs on metastatic OSCC cells, which warrants further in vivo investigations.
Adaptive hybrid high-order method for guaranteed lower eigenvalue bounds
Authors: Carstensen Carsten , Gräßle Benedikt , Tran Ngoc Tien
Published: May 2024

The higher-order guaranteed lower eigenvalue bounds of the Laplacian in the recent work by Carstensen et al. (Numer Math 149(2):273–304, 2021) require a parameter $$C_{\text {st},1}$$

C

st
,
1

that is found not robust as the polynomial degree p increases. This is related to the $$H^1$$

H
1

stability bound of the $$L^{2}$$

L
2

projection onto polynomials of degree at most p and its growth $$C_{\textrm{st, 1}}\propto (p+1)^{1/2}$$

C

st, 1

∝

(
p
+
1
)

1
/
2

as $$p \rightarrow \infty $$

p
→
∞

. A similar estimate for the Galerkin projection holds with a p-robust constant $$C_{\text {st},2}$$

C

st
,
2

and $$C_{\text {st},2} \le 2$$

C

st
,
2

≤
2

for right-isosceles triangles. This paper utilizes the new inequality with the constant $$C_{\text {st},2}$$

C

st
,
2

to design a modified hybrid high-order eigensolver that directly computes guaranteed lower eigenvalue bounds under the idealized hypothesis of exact solve of the generalized algebraic eigenvalue problem and a mild explicit condition on the maximal mesh-size in the simplicial mesh. A key advance is a p-robust parameter selection. The analysis of the new method with a different fine-tuned volume stabilization allows for a priori quasi-best approximation and improved $$L^{2}$$

L
2

error estimates as well as a stabilization-free reliable and efficient a posteriori error control. The associated adaptive mesh-refining algorithm performs superior in computer benchmarks with striking numerical evidence for optimal higher empirical convergence rates.
Towards controlled and simple design of non-enzymatic amperometric sensor for glycerol determination in yeast fermentation medium
Authors: Zolotukhina E. V. , Butyrskaya E. V. , Fink-Straube C. , Koch M. , Silina Y. E.
Published: May 2024

Glycerol is a widely used signaling bioanalyte in biotechnology. Glycerol can serve as a substrate or product of many metabolic processes in cells. Therefore, quantification of glycerol in fermentation samples with inexpensive, reliable, and rapid sensing systems is of great importance. In this work, an amperometric assay based on one-step designed electroplated functional Pd layers with controlled design was proposed for a rapid and selective measurement of glycerol in yeast fermentation medium. A novel assay utilizing electroplated Pd-sensing layers allows the quantification of glycerol in yeast fermentation medium in the presence of interfering species with RSD below 3% and recoveries ranged from 99 to 103%. The assay requires minimal sample preparation, viz. adjusting of sample pH to 12. The time taken to complete the electrochemical analysis was 3 min. Remarkably, during investigations, it was revealed that sensitivity and selectivity of glycerol determination on Pd sensors were significantly affected by its adsorption and did not depend on the surface structure of sensing layers. This study is expected to contribute to both fundamental and practical research fields related to a preliminary choice of functional sensing layers for specific biotechnology and life science applications in the future.

Graphical Abstract
Community Assembly Processes of Deadwood Mycobiome in a Tropical Forest Revealed by Long-Read Third-Generation Sequencing
Authors: Purahong Witoon , Ji Li , Wu Yu-Ting
Published: May 2024

Despite the importance of wood-inhabiting fungi on nutrient cycling and ecosystem functions, their ecology, especially related to their community assembly, is still highly unexplored. In this study, we analyzed the wood-inhabiting fungal richness, community composition, and phylogenetics using PacBio sequencing. Opposite to what has been expected that deterministic processes especially environmental filtering through wood-physicochemical properties controls the community assembly of wood-inhabiting fungal communities, here we showed that both deterministic and stochastic processes can highly contribute to the community assembly processes of wood-inhabiting fungi in this tropical forest. We demonstrated that the dynamics of stochastic and deterministic processes varied with wood decomposition stages. The initial stage was mainly governed by a deterministic process (homogenous selection), whereas the early and later decomposition stages were governed by the stochastic processes (ecological drift). Deterministic processes were highly contributed by wood physicochemical properties (especially macronutrients and hemicellulose) rather than soil physicochemical factors. We elucidated that fine-scale fungal-fungal interactions, especially the network topology, modularity, and keystone taxa of wood-inhabiting fungal communities, strongly differed in an initial and decomposing deadwood. This current study contributes to a better understanding of the ecological processes of wood-inhabiting fungi in tropical regions where the knowledge of wood-inhabiting fungi is highly limited.
Heat stress-induced NO enhanced perylenequinone biosynthesis of Shiraia sp. via calcium signaling pathway
Authors: Bao Zhuanying , Chen Yunni , Zhang Zhibin , Yang Huilin , Yan Riming , Zhu Du
Published: May 2024

Perylenequinones (PQs) are natural photosensitizing compounds used as photodynamic therapy, and heat stress (HS) is the main limiting factor of mycelial growth and secondary metabolism of fungi. This study aimed to unravel the impact of HS-induced Ca2+ and the calcium signaling pathway on PQ biosynthesis of Shiraia sp. Slf14(w). Meanwhile, the intricate interplay between HS-induced NO and Ca2+ and the calcium signaling pathway was investigated. The outcomes disclosed that Ca2+ and the calcium signaling pathway activated by HS could effectively enhance the production of PQs in Shiraia sp. Slf14(w). Further investigations elucidated the specific mechanism through which NO signaling molecules induced by HS act upon the Ca2+/CaM (calmodulin) signaling pathway, thus propelling PQ biosynthesis in Shiraia sp. Slf14(w). This was substantiated by decoding the downstream positioning of the CaM/CaN (calcineurin) pathway in relation to NO through comprehensive analyses encompassing transcript levels, enzyme assays, and the introduction of chemical agents. Concurrently, the engagement of Ca2+ and the calcium signaling pathway in heat shock signaling was also evidenced. The implications of our study underscore the pivotal role of HS-induced Ca2+ and the calcium signaling pathway, which not only participate in heat shock signal transduction but also play an instrumental role in promoting PQ biosynthesis. Consequently, our study not only enriches our comprehension of the mechanisms driving HS signaling transduction in fungi but also offers novel insights into the PQ synthesis paradigm within Shiraia sp. Slf14(w).

Key points

• The calcium signaling pathway was proposed to participate in PQ biosynthesis under HS.

• HS-induced NO was revealed to act upon the calcium signaling pathway for the first time.
Dental plaque microbiota sequence counts for microbial profiling and resistance genes detection
Authors: Veschetti Laura , Paiella Salvatore , Carelli Maria , Zotti Francesca , Secchettin Erica , Malleo Giuseppe , Signoretto Caterina , Zulianello Giorgia , Nocini Riccardo , Crovetto Anna , Salvia Roberto , Bassi Claudio , Malerba Giovanni
Published: May 2024

Shotgun metagenomics sequencing experiments are finding a wide range of applications. Nonetheless, there are still limited guidelines regarding the number of sequences needed to acquire meaningful information for taxonomic profiling and antimicrobial resistance gene (ARG) identification. In this study, we explored this issue in the context of oral microbiota by sequencing with a very high number of sequences (~ 100 million), four human plaque samples, and one microbial community standard and by evaluating the performance of microbial identification and ARGs detection through a downsampling procedure. When investigating the impact of a decreasing number of sequences on quantitative taxonomic profiling in the microbial community standard datasets, we found some discrepancies in the identified microbial species and their abundances when compared to the expected ones. Such differences were consistent throughout downsampling, suggesting their link to taxonomic profiling methods limitations. Overall, results showed that the number of sequences has a great impact on metagenomic samples at the qualitative (i.e., presence/absence) level in terms of loss of information, especially in experiments having less than 40 million reads, whereas abundance estimation was minimally affected, with only slight variations observed in low-abundance species. The presence of ARGs was also assessed: a total of 133 ARGs were identified. Notably, 23% of them inconsistently resulted as present or absent across downsampling datasets of the same sample. Moreover, over half of ARGs were lost in datasets having less than 20 million reads. This study highlights the importance of carefully considering sequencing aspects and suggests some guidelines for designing shotgun metagenomics experiments with the final goal of maximizing oral microbiome analyses. Our findings suggest varying optimized sequence numbers according to different study aims: 40 million for microbiota profiling, 50 million for low-abundance species detection, and 20 million for ARG identification.

Key points

• Forty million sequences are a cost-efficient solution for microbiota profiling

• Fifty million sequences allow low-abundance species detection

• Twenty million sequences are recommended for ARG identification

Graphical Abstract
Bone morphology and physical characteristics of the pro-cyclist hip joint
Authors: Akiho Shunsuke , Hashida Ryuki , Tagawa Yoshihiko , Maeyama Akira , Kinoshita Koichi , Kanazawa Kazuki , Matsuse Hiroo , Hara Masafumi , Yamamoto Takuaki
Published: May 2024

Purpose

This study aimed to investigate the radiographic findings for the hip joint and hip range of motion in professional cyclists, and to determine their bone morphology and physical characteristics. The effects of physical characteristics on athletic performance were examined in terms of metabolic efficiency using simulation analysis.

Methods

We performed a case–control research study on 22 hips in 11 male professional cyclists (average age 28.5, height 1.73 m, weight 77.6 kg). Thirty hips in 15 healthy male volunteers were selected as controls. As radiographic evaluations, acetabular dysplasia was assessed on standardized radiographs. During physical evaluations, the hip range of motion was examined. We used simulation analysis to investigate the metabolic efficiency in the different cycling forms.

Results

The radiographic evaluations showed a significant difference in the incidence of acetabular dysplasia (p = 0.01): 59% (13/22 hips) in the pro-cyclist group versus 10% (3/30 hips) in the control group. The physical evaluations revealed significant differences in the hip internal rotation angle (p = 0.01), with greater ranges of internal rotation in the pro-cyclist group versus the control group. The simulation analyses showed that metabolism was reduced in the cycling form with hip internal rotation, especially in the lower extremities.

Conclusions

Pro-cyclists showed a high frequency of acetabular dysplasia and superior hip internal rotation. According to the cycling model analyses, hip internal rotation allowed pedaling with reduced metabolic power.
Predicting clinically significant prostate cancer following suspicious mpMRI: analyses from a high-volume center
Authors: Jahnen Matthias , Hausler Tanja , Meissner Valentin H. , Ankerst Donna P. , Kattan Michael W. , Sauter Andreas , Gschwend Juergen E. , Herkommer Kathleen
Published: May 2024

Purpose

mpMRI is routinely used to stratify the risk of clinically significant prostate cancer (csPCa) in men with elevated PSA values before biopsy. This study aimed to calculate a multivariable risk model incorporating standard risk factors and mpMRI findings for predicting csPCa on subsequent prostate biopsy.

Methods

Data from 677 patients undergoing mpMRI ultrasound fusion biopsy of the prostate at the TUM University Hospital tertiary urological center between 2019 and 2023 were analyzed. Patient age at biopsy (67 (median); 33–88 (range) (years)), PSA (7.2; 0.3–439 (ng/ml)), prostate volume (45; 10–300 (ml)), PSA density (0.15; 0.01–8.4), PI-RADS (V.2.0 protocol) score of index lesion (92.2% ≥3), prior negative biopsy (12.9%), suspicious digital rectal examination (31.2%), biopsy cores taken (12; 2–22), and pathological biopsy outcome were analyzed with multivariable logistic regression for independent associations with the detection of csPCa defined as ISUP ≥ 3 (n = 212 (35.2%)) and ISUP ≥ 2 (n = 459 (67.8%) performed on 603 patients with complete information.

Results

Older age (OR: 1.64 for a 10-year increase; p < 0.001), higher PSA density (OR: 1.60 for a doubling; p < 0.001), higher PI-RADS score of the index lesion (OR: 2.35 for an increase of 1; p < 0.001), and a prior negative biopsy (OR: 0.43; p = 0.01) were associated with csPCa.

Conclusion

mpMRI findings are the dominant predictor for csPCa on follow-up prostate biopsy. However, PSA density, age, and prior negative biopsy history are independent predictors. They must be considered when discussing the individual risk for csPCa following suspicious mpMRI and may help facilitate the further diagnostical approach.
Value of perilesional biopsies in multiparametric magnetic resonance imaging-targeted biopsy and systematic biopsy in detection of prostate cancer: results of a prospective, non-randomized, surgeon-blinded study
Authors: Duwe Gregor , Schmitteckert Melanie , Haack Maximilian , Sparwasser Peter , Dotzauer Robert , Thomas Anita , Tsaur Igor , Brandt Maximilian Peter , Kurosch Martin , Mager Rene , Haferkamp Axel , Boehm Katharina , Höfner Thomas
Published: May 2024

Purpose

The goal of this study is to address if detection rates of clinically significant prostate cancer (csPCa) can be increased by additional perilesional biopsies (PB) in magnetic resonance (MR)/ultrasound fusion prostate biopsy in biopsy-naïve men.

Methods

This prospective, non-randomized, surgeon-blinded study was conducted between February 2020 and July 2022. Patients were included with PSA levels < 20 ng/ml and ≥ one PI-RADS lesion (grades 3–5) per prostate lobe. Prostate biopsy was performed by two urologists. The first performed the MR-fusion biopsy with 3–5 targeted biopsies (TB) and 6 PB in a standardized pattern. The second performed the systematic (12-fold) biopsy (SB) without knowledge of the MR images. Primary outcome of this study is absence or presence of csPCa (≥ ISUP grade 2) comparing TB, PB and SB, using McNemar test.

Results

Analyses were performed for each PI-RADS lesion (n = 218). There was a statistically significant difference in csPC detection rate of TB + SB between PI-RADS 3, 4 and 5 lesions (18.0% vs. 42.5% vs. 82.6%, p < 0.001) and TB + PB (19.7% vs. 29.1% vs. 78.3%). Comparing only maximum ISUP grade per lesion, even SB plus TB plus PB did not detect more csPCa compared to SB plus TB (41.3% vs. 39.9%, p > 0.05).

Conclusion

We present prospective study data investigating the role of perilesional biopsy in detection of prostate cancer. We detected no statistically significant difference in the detection of csPCa by the addition of PB. Therefore, we recommend continuing 12-fold bilateral SB in addition to TB.
Downscaling the ocean response to the Madden–Julian Oscillation in the Northwest Atlantic and adjacent shelf seas
Authors: Renkl Christoph , Oliver Eric C. J. , Thompson Keith R.
Published: May 2024

Subseasonal-to-seasonal (S2S) prediction is a global effort to forecast the state of the atmosphere and ocean with lead times between two weeks and a season. This study explores the feasibility of S2S prediction of the ocean using a variety of tools including statistical analysis, a statistical-dynamical mixed layer model, and a regional, high-resolution ocean circulation model based on physical principles. Ocean predictability on S2S timescales is analyzed by compositing winter sea surface temperature (SST) anomalies in the North Atlantic with respect to the state of the Madden–Julian Oscillation (MJO). It is found that statistically significant, large-scale SST changes, particularly along the eastern seaboard of North America, can be related to the MJO. This signal is shown to be driven by anomalous air–sea heat fluxes caused by atmospheric perturbations in response to the MJO. The high-resolution model of the Gulf of Maine and Scotian Shelf is used to downscale the mean ocean response to the MJO. The model is able to capture the observed relationship between the MJO and SST in the northwest Atlantic. It is also shown that the anomalous atmospheric circulation in response to the MJO leads to anomalous upwelling on the Scotian Shelf. Overall, this study demonstrates that it is feasible, and of value, to use regional ocean models for S2S prediction.
Total hysterectomy versus radical hysterectomy in neuroendocrine cervical cancer: a SEER-database analysis
Authors: Zeng Feitianzhi , Guo Peng , Xia Meng , He Mian
Published: May 2024

Purpose

We conducted this study to evaluate the efficacy of total hysterectomy versus radical hysterectomy in the treatment of neuroendocrine cervical cancer (NECC).

Methods

Eligible NECC patients were identified from the Surveillance, Epidemiology and End Results (SEER) database. Demographic characteristics, clinical treatment and survival of the patients were collected. The overall survival (OS) and cancer-specific survival (CSS) were estimated by Kaplan–Meier analysis with log-rank test.

Results

A total of 286 patients were included, with 104 patients undergoing total hysterectomy and 182 patients undergoing radical hysterectomy. The 5-year OS were 50.8% in the total hysterectomy group and 47.5% in the radical hysterectomy group (p = 0.450); and the corresponding 5-year CSS were 51.6% and 49.1% (p = 0.494), respectively. Along with surgery, radiotherapy was given to 49.0% of patients in the total hysterectomy group and 50.5% in the radical hysterectomy group; and chemotherapy was administered to 77.9% of patients in the total hysterectomy group and 85.7% in the radical hysterectomy group. Unexpectedly, in patients who received adjuvant radiotherapy with or without chemotherapy, the OS was superior in the total hysterectomy group compared with the radical hysterectomy group (p = 0.034). While in patients who received chemotherapy alone and those who received neither radiotherapy nor chemotherapy, the OS still remained comparable between the total hysterectomy and radical hysterectomy group.

Conclusion

Compared with radical hysterectomy, total hysterectomy was not associated with compromised survival prognosis in patients with NECC. Total hysterectomy has the potential to be a surgical alternative in the multimodal management of NECC.
A thermodynamically consistent phase transformation model for multiphase alloys: application to Ti$$_6$$Al$$_4$$V in laser powder bed fusion processes
Authors: Noll Isabelle , Bartel Thorsten , Menzel Andreas
Published: May 2024

Titan aluminium alloys belong to the group of $$\alpha $$
α
–$$\beta $$
β
-alloys, which are used for many applications in industry due to their advantageous mechanical properties, e.g. for laser powder bed fusion (PBF-LB) processes. However, the composition of the crystal structure and the respective magnitude of the solid fraction highly influences the material properties of titan aluminium alloys. Specifically, the thermal history, i.e. the cooling rate, determines the phase composition and microstructure for example during heat treatment and PBF-LB processes. For that reason, the present work introduces a phase transformation framework based, amongst others, on energy densities and thermodynamically consistent evolution equations, which is able to capture the different material compositions resulting from cooling and heating rates. The evolution of the underlying phases is governed by a specifically designed dissipation function, the coefficients of which are determined by a parameter identification process based on available continuous cooling temperature (CCT) diagrams. In order to calibrate the model and its preparation for further applications such as the simulation of additive manufacturing processes, these CCT diagrams are computationally reconstructed. In contrast to empirical formulations, the developed thermodynamically consistent and physically sound model can straightforwardly be extended to further phase fractions and different materials. With this formulation, it is possible to predict not only the microstructure evolution during processes with high temperature gradients, as occurring in e.g. PBF-LB processes, but also the evolving strains during and at the end of the process.
Evaluation of optical coherence tomography angiography metrics in children and adolescents with type 1 diabetes: 4-year longitudinal study
Authors: Qin Xinran , Xiao Ying , Cui Lipu , Chen Shuli , An Qingyu , Yuan Tianyi , Wu Yiwei , Lin Qiurong , Yang Chenhao , Zou Haidong
Published: May 2024

Purpose

To evaluate longitudinal changes in optical coherence tomography angiography (OCTA) metrics in children and adolescents with type 1 diabetes (T1D).

Methods

This prospective observational cohort study included thirty-two eyes from thirty T1D children with no history of diabetic retinopathy (DR) who were followed up for 4 years. Participants underwent OCTA examinations at baseline and during follow-up. Quantitative OCTA metrics were measured using a customized MATLAB algorithm. Generalized mixed-effect models were used to determine their relationship with DR development. Systemic parameters and OCTA metrics were screened using least absolute shrinkage and selection operator to identify predictors for visual function.

Results

Over the 4-year period, seven of the included eyes developed DR, and most OCTA metrics decreased with diabetes duration. Higher peripapillary and parafoveal nasal quadrant vessel area density (VAD) in the superficial capillary plexus (SCP) and vessel skeleton density (VSD) in both the SCP and the deep capillary plexus (DCP) were associated with a lower risk of DR in T1D. Parafoveal DCP VSD and VAD in the temporal and inferior quadrants were anticorrelated with changes in best corrected visual acuity.

Conclusions

OCTA metrics dynamically change over the duration of diabetes and can be used as biomarkers to improve the risk evaluation of DR development and visual function in T1D children and adolescents.
Investigation of the fungiform papillae number in children with tooth number anomalies
Authors: Alp Belgin , Kalaoglu Elif Ece , Mentes Ali
Published: May 2024

Objective

This cross-sectional study investigated the association between fungiform papillae (FP) numbers and tooth number anomalies in children, considering variables related to hypodontia and hyperdontia. The aim was to explore this association while adjusting for age and sex differences.

Materials and methods

A total of 144 children (aged 8–10) were categorized into hypodontia (n = 48), hyperdontia (n = 48), and control groups (n = 48). Clinical and radiographic diagnoses were used to classify tooth number anomalies. Hypodontia was categorized by number and location, while hyperdontia was categorized by number, shape, and location. FP were assessed using the Denver Papillae Protocol. Data analyses were performed using NCSS software, with p < 0.05 considered statistically significant.

Results

The hypodontia group (22.5 ± 8.4) exhibited significantly lower FP than the control group (30.4 ± 9.2) and the hyperdontia group (27.9 ± 7.8) (p < 0.0005, p = 0.003, respectively). No significant difference existed between the hyperdontia and control groups. FP numbers in hypodontia subgroups showed no significant differences based on teeth agenesis numbers or locations. Similarly, hyperdontia subgroup analyses revealed no significant differences in FP numbers based on supernumerary teeth shapes (supplemental, conical, tuberculoid, paramolar) or the numbers of supernumerary teeth.

Conclusions

The lower FP numbers in children with hypodontia suggested an association between teeth and FP number. However, the non-significant difference in FP numbers with hyperdontia underscored the complexity of tooth development, warranting further investigations.

Clinical relevance

Children with hypodontia may exhibit distinct FP numbers compared to those without tooth number anomalies.
Transforming labour around food? the experience of community supported agriculture in Italy
Authors: Rossi Adanella , Piccoli Alessandra , Feola Giuseppe
Published: May 2024

This study examines the strategies developed by Italian Community Supported Agriculture (CSA) initiatives to de-commodify, de-instrumentalise and de-monetise labour in their attempt to prefigure alternatives to dominant capitalist agri-food systems. To do so we answer the following research questions: What type of strategies do these CSA initiatives employ to that end? What insights can be gained as regards the potential and barriers to fuller and more accomplished post-capitalist labour management within CSA initiatives? Data were collected through a participatory action research project involving 16 Italian CSAs, participant observation in meetings of CSA initiatives and the Italian CSA Network, and official documents collection. Across the three crucial moments considered in this study, we observe substantial difficulties in realising post-capitalist labour management as the initiatives are constrained by external requirements (e.g., legislation) and also internally by members’ weakness in implementing the CSA model due to their remaining integrated in the capitalist system. These findings point to the importance for CSA initiatives of deepening the collective process of deliberate deconstruction of valuation logics and predefined roles, as well as legal frameworks to find ways to enable the realisation of post-capitalist labour management. In this regard, we also suggest that the Italian CSA Network could play a more active role in addressing some of the internal and external factors hindering the consolidation of CSA initiatives and the manifestation of their transformative role.
Synthesis and active manipulation of magnetic liquid beads
Authors: Yadav Ajeet Singh , Galogahi Fariba Malekpour , Vashi Aditya , Tran Du Tuan , Kijanka Gregor S. , Cha Haotian , Sreejith Kamalalayam Rajan , Nguyen Nam-Trung
Published: May 2024

We report the fabrication and characterisation of magnetic liquid beads with a solid magnetic shell and liquid core using microfluidic techniques. The liquid beads consist of a fluorinated oil core and a polymer shell with magnetite particles. The beads are generated in a flow-focusing polydimethylsiloxane (PDMS) device and cured by photo polymerisation. We investigated the response of the liquid beads to an external magnetic field by characterising their motion towards a permanent magnet. Magnetic sorting of liquid beads in a channel was achieved with 90% efficiency. The results show that the liquid beads can be controlled magnetically and have potential applications in digital microfluidics including nucleic acid amplification, drug delivery, cell culture, sensing, and tissue engineering. The present paper also discusses the magnetophoretic behaviour of the liquid bead by varying its mass and magnetite concentration in the shell. We also demonstrated the two-dimensional self-assembly of magnetic liquid beads for potential use in digital polymerase chain reaction and digital loop mediated isothermal amplification.

Graphical abstract
A novel approach detection for IIoT attacks via artificial intelligence
Authors: Karacayılmaz Gökçe , Artuner Harun
Published: May 2024

The Industrial Internet of Things (IIoT) is a paradigm that enables the integration of cyber-physical systems in critical infrastructures, such as power grids, water distribution networks, and transportation systems. IIoT devices, such as sensors, actuators, and controllers, can provide various benefits, such as performance optimization, efficiency improvement, and remote management. However, these devices also pose new security risks and challenges, as they can be targeted by malicious actors to disrupt the normal operation of the infrastructures they are connected to or to cause physical damage or harm. Therefore, it is essential to develop effective and intelligent solutions to detect and prevent attacks on IIoT devices and to ensure the security and resilience of critical infrastructures. In this paper, we present a comprehensive analysis of the types and impacts of attacks on IIoT devices based on a literature review and a data analysis of real-world incidents. We classify the attacks into four categories: denial-of-service, data manipulation, device hijacking, and physical tampering. We also discuss the potential consequences of these attacks on the safety, reliability, and availability of critical infrastructures. We then propose an expert system that can detect and prevent attacks on IIoT devices using artificial intelligence techniques, such as rule-based reasoning, anomaly detection, and reinforcement learning. We describe the architecture and implementation of our system, which consists of three main components: a data collector, a data analyzer, and a data actuator. We also present a table that summarizes the main features and capabilities of our system compared to existing solutions. We evaluate the performance and effectiveness of our system on a testbed consisting of programmable logic controllers (PLCs) and IIoT protocols, such as Modbus and MQTT. We simulate various attacks on IIoT devices and measure the accuracy, latency, and overhead of our system. Our results show that our system can successfully detect and mitigate different types of attacks on IIoT devices with high accuracy and low latency and overhead. We also demonstrate that our system can enhance the security and resilience of critical infrastructures by preventing or minimizing the impacts of attacks on IIoT devices.
Ethics in AI through the practitioner’s view: a grounded theory literature review
Authors: Pant Aastha , Hoda Rashina , Tantithamthavorn Chakkrit , Turhan Burak
Published: May 2024

The term ethics is widely used, explored, and debated in the context of developing Artificial Intelligence (AI) based software systems. In recent years, numerous incidents have raised the profile of ethical issues in AI development and led to public concerns about the proliferation of AI technology in our everyday lives. But what do we know about the views and experiences of those who develop these systems – the AI practitioners? We conducted a grounded theory literature review (GTLR) of 38 primary empirical studies that included AI practitioners’ views on ethics in AI and analysed them to derive five categories: practitioner awareness, perception, need, challenge, and approach. These are underpinned by multiple codes and concepts that we explain with evidence from the included studies. We present a taxonomy of ethics in AI from practitioners’ viewpoints to assist AI practitioners in identifying and understanding the different aspects of AI ethics. The taxonomy provides a landscape view of the key aspects that concern AI practitioners when it comes to ethics in AI. We also share an agenda for future research studies and recommendations for practitioners, managers, and organisations to help in their efforts to better consider and implement ethics in AI.
On the stability of norms and norm-following propensity: a cross-cultural panel study with adolescents
Authors: Kimbrough Erik O. , Krupka Erin L. , Kumar Rajnish , Murray Jennifer M. , Ramalingam Abhijit , Sánchez-Franco Sharon , Sarmiento Olga L. , Kee Frank , Hunter Ruth F.
Published: May 2024

Norm-based accounts of social behavior in economics typically reflect tradeoffs between maximization of own consumption utility and conformity to social norms. Theories of norm-following tend to assume that there exists a single, stable, commonly known injunctive social norm for a given choice setting and that each person has a stable propensity to follow social norms. We collect panel data on 1468 participants aged 11–15 years in Belfast, Northern Ireland and Bogotá, Colombia in which we measure norms for the dictator game and norm-following propensity twice at 10 weeks apart. We test these basic assumptions and find that norm-following propensity is stable, on average, but reported norms show evidence of change. We find that individual-level variation in reported norms between people and within people across time has interpretable structure using a series of latent transition analyses (LTA) which extend latent class models to a panel setting. The best fitting model includes five latent classes corresponding to five sets of normative beliefs that can be interpreted in terms of what respondents view as “appropriate” (e.g. equality vs. generosity) and how they view deviations (e.g. deontological vs. consequentialist). We also show that a major predictor of changing latent classes over time comes from dissimilarity to others in one’s network. Our application of LTA demonstrates how researchers can engage with heterogeneity in normative perceptions by identifying latent classes of beliefs and deepening understanding of the extent to which norms are shared, stable, and can be predicted to change. Finally, we contribute to the nascent experimental literature on the economic behavior of children and adolescents.
A Simplified Method for Determining Fire Resistance of RC Columns Using Fire-Resistance-Column-Curves Approach
Authors: Mahmoud Khaled Ahmed
Published: May 2024

Eurocode-2 (EC2) empirical equation for fire resistance of RC columns is very sensitive to the value of column capacity at normal temperature conditions $$N_{Rd}$$

N

Rd

. Techniques to determine $$N_{Rd}$$

N

Rd

accurately for eccentric slender columns are difficult and computationally demanding; thus, adopting simplifications leads to unsatisfactory results in many column cases. Another shortcoming of EC2 equation is that it does not include an explicit term regarding the effect of load eccentricity on fire resistance. In this paper, a simplified method, as an attempt to overcome EC2 method defects, is developed to determine the fire resistance of RC columns using fire-resistance-column-curves. A rational numerical model is used to analyze various series of RC columns with different geometric, material, and loading properties at elevated temperatures. The results of the numerical study are utilized to construct different fire-resistance-column-curves from which simplified design equations are developed to predict the fire resistance of fixed- and pinned-end RC columns. The validity of the method is established with the aid of experimental data and it was found that in most cases, there is good agreement between assessed and test columns. It was also found that the proposed equations provide sufficiently safe predictions when appropriate material safety factors are adopted. The applicability of the proposed method to fire resistance design of RC columns is illustrated through numerical examples.
Adolescent Residential Mobility and Life Satisfaction in Emerging Adulthood
Authors: Henkens Juul H. D. , Kalmijn Matthijs , de Valk Helga A. G.
Published: May 2024

Moving in adolescence could be a stressful experience, potentially disturbing development into adulthood. This study investigated the relationship between residential mobility in adolescence and life satisfaction in young adulthood, focusing on moving timing, frequency, and distance, using (retrospective) longitudinal German survey data (N = 2998, mean age = 20.18, 45.8% boy, 6.8% first-generation immigrant, 31.0% children of immigrants). Moving twice or more in adolescence was linked to lower life satisfaction in young adulthood, even after controlling for family background characteristics. Moving distance was unrelated to life satisfaction. Exploratory analyses showed that particularly for children of immigrants, frequent moving was related to lower life satisfaction. Since frequent moving in adolescence was more common in disadvantaged families, frequent moving could reinforce social inequalities into young adulthood.
Principal Component Analysis for Distributions Observed by Samples in Bayes Spaces
Authors: Pavlů Ivana , Machalová Jitka , Tolosana-Delgado Raimon , Hron Karel , Bachmann Kai , van den Boogaart Karl Gerald
Published: May 2024

Distributional data have recently become increasingly important for understanding processes in the geosciences, thanks to the establishment of cost-efficient analytical instruments capable of measuring properties over large numbers of particles, grains or crystals in a sample. Functional data analysis allows the direct application of multivariate methods, such as principal component analysis, to such distributions. However, these are often observed in the form of samples, and thus incur a sampling error. This additional sampling error changes the properties of the multivariate variance and thus the number of relevant principal components and their direction. The result of the principal component analysis becomes an artifact of the sampling error and can negatively affect the subsequent data analysis. This work presents a way of estimating this sampling error and how to confront it in the context of principal component analysis, where the principal components are obtained as a linear combination of elements of a newly constructed orthogonal spline basis. The effect of the sampling error and the effectiveness of the correction is demonstrated with a series of simulations. It is shown how the interpretability and reproducibility of the principal components improve and become independent of the selection of the basis. The proposed method is then applied on a dataset of grain size distributions in a geometallurgical dataset from Thaba mine in the Bushveld complex.
Gatekeeping should be conserved in the open science era
Authors: Desmond Hugh
Published: May 2024

The elimination of gatekeepers for scientific publication has been represented as a means to promote the core moral values of open science, including democratic decision-making and inclusiveness. I argue that this framing ignores the reality that gatekeeping is a way of structuring prestige hierarchies, and that without gatekeeping, some other structuring would be needed: the flattening of prestige hierarchies is not possible given scientists’ need to navigate information overload. I consider two potential restructurings of prestige hierarchies, one based on citation count and the other on search algorithm rank. These are shown to simply reintroduce status biases and hierarchies in ways that either do not further the open science ideals of democracy and inclusiveness, or else involve some de facto gatekeeping. Gatekeeper elimination should not be thought of as an intrinsic part of the open science movement. In fact, insofar as gatekeeping is guided by professional ideals of impartiality and diligence, it can be thought of as an ally of open science values.
Intraoperative haemoadsorption for antithrombotic drug removal during cardiac surgery: initial report of the international safe and timely antithrombotic removal (STAR) registry
Authors: Schmoeckel Michael , Thielmann Matthias , Hassan Kambiz , Geidel Stephan , Schmitto Jan , Meyer Anna L. , Vitanova Keti , Liebold Andreas , Marczin Nandor , Bernardi Martin H. , Tandler Rene , Lindstedt Sandra , Matejic-Spasic Marijana , Wendt Daniel , Deliargyris Efthymios N. , Storey Robert F.
Published: May 2024

Intraoperative antithrombotic drug removal by haemoadsorption is a novel strategy to reduce perioperative bleeding in patients on antithrombotic drugs undergoing cardiac surgery. The international STAR registry reports real-world clinical outcomes associated with this application. All patients underwent cardiac surgery before completing the recommended washout period. The haemoadsorption device was incorporated into the cardiopulmonary bypass (CPB) circuit. Patients on P2Y12 inhibitors comprised group 1, and patients on direct-acting oral anticoagulants (DOAC) group 2. Outcome measurements included bleeding events according to standardised definitions and 24-hour chest-tube-drainage (CTD). 165 patients were included from 8 institutions in Austria, Germany, Sweden, and the UK. Group 1 included 114 patients (62.9 ± 11.6years, 81% male) operated at a mean time of 33.2 h from the last P2Y12 inhibitor dose with a mean CPB duration of 117.1 ± 62.0 min. Group 2 included 51 patients (68.4 ± 9.4years, 53% male), operated at a mean time of 44.6 h after the last DOAC dose, with a CPB duration of 128.6 ± 48.4 min. In Group 1, 15 patients experienced a BARC-4 bleeding event (13%), including 3 reoperations (2.6%). The mean 24-hour CTD was 651 ± 407mL. In Group 2, 8 patients experienced a BARC-4 bleeding event (16%) including 4 reoperations (7.8%). The mean CTD was 675 ± 363mL. This initial report of the ongoing STAR registry shows that the intraoperative use of a haemoadsorption device is simple and safe, and may potentially mitigate the expected high bleeding risk of patients on antithrombotic drugs undergoing cardiac surgery before completion of the recommended washout period.

Clinical registration number: ClinicalTrials.gov identifier: NCT05077124.

Graphical Abstract
Voice Behaviours within Cooperatives. The Importance of the Relational Aspects of Leadership and Integrative Mechanisms for Loyalty
Authors: Mori Martina , Cavaliere Vincenzo
Published: May 2024

This study explores the dynamics of cooperatives, with a focus on the internal challenges associated with sustaining democratic governance and promoting active member participation. The emphasis is on individual-level participation within the workplace, an often-neglected aspect in cooperative literature. An empirical model examines how member loyalty influences constructive and destructive voice behaviours. Leader–member exchange (LMX) relationship and integrative mechanisms are the proposed mediators in this context. Analysing data from 301 members of 19 worker cooperatives in Italy revealed different mediating effects through LMX, integrative mechanisms and a combined sequence. These findings enrich the cooperative literature by highlighting the importance of loyalty and voice behaviours in regenerating cooperative principles and member participation. The study also underscores the role of interpersonal relationships in affecting operational processes and explaining members’ loyalty and participation dynamics, proposing suggestions for cooperative management upholding democratic governance rooted in trust-based relationships, organic organisational models and mutual values.
Environmental and Food Contamination by Phthalic Acid Esters (PAEs): Overview
Authors: Sokołowski Artur , Kończak Magdalena , Oleszczuk Patryk , Gao Yanzheng , Czech Bożena
Published: May 2024

Phthalic acid esters (PAEs) are dialkyl or alkyl/aryl ester derivatives of phthalic acid. PAEs are colorless, odorless, and flavorless oily liquids. PAEs are the main plasticizers used in industry and households. DEHP (di-(2-ethyl hexyl) phthalate) is the main plasticizer used in the polymer industry, whereas DMP (dimethyl phthalate) and DEP (diethyl phthalate) are used mainly as solvents or fixatives in cosmetics and personal care products. PAEs are synthetic organic compounds poorly soluble in water but soluble in organic solvents. Into the environment, they are introduced during the production, use and degradation, packaging, and transportation of plastic products. In the environment, PAEs are degraded in three ways: by hydrolysis, photodegradation, and microbial degradation, whereas the biodegradation and hydrolysis of PAEs in the environment are very slow. PAEs are pollutants of soil, water, groundwater, river water, marine water, air, sediments, vegetables, and biota. Due to the great interest in the subject of environmental pollution by PAEs and the emergence of new information in this area, it is extremely important to systematically review the current knowledge. In the presented paper, the occurrence of PAEs in different environmental matrices was reviewed. The toxicity to plants, animals, and humans was also described.
Venture capital internationalization in China and the Uppsala model
Authors: Hudson, Paul L. , Richardson James
Published: May 2024

We study the investment behavior of new domestic Chinese venture capital (VC) firms compared to foreign VC firms newly entering the Chinese market. Given the institutional and cultural differences or psychic distance between China and the home country of foreign VC’s, the Uppsala Model would predict that foreign VC’s will be more cautious initially than domestic Chinese VC’s and that the degree of caution will increase with the psychic distance. Our data comes primarily from Zero2IPO, which has a nearly exhaustive list of VC investments in China from 1996 to 2006. We also use country-of-origin, membership in GLOBE cultural clusters, and a broad measure of psychic distance based on institutional and cultural differences from Berry, Guillén, & Zhou (2010) and the Freedom Index to test this prediction. We find support for the hypothesis that psychic distance affects initial investment behavior.