bluuebunny/crossref_metadata_2025 huggingface dataset:
Motion events in Swedish and French: a Holistic Spatial Semantics analysis
Authors: Vesnina Nataliia
Published: February 2024

The present study investigates cross-linguistic differences in the description of motion events using Holistic Spatial Semantics (HSS) as a theoretical framework. In this study, six short video stimuli featuring various motion situations were used to elicit narratives from 35 speakers of French and 29 speakers of Swedish. The proportions of semantic category Path linguistically expressed did not vary significantly between the groups. However, the French narratives had significantly less Manner and Direction expression. Furthermore, they included significantly more unbounded non-translocative events compared to the Swedish narratives. Partly, this was due to the tendency within the Swedish group to construe situations as bounded events that were clearly shown as unbounded in the stimuli. There was also a tendency within the French group to distribute the information about the same situation over two clauses. These findings lend further support to the relevance of distinguishing between the Path and Direction categories, as well as other key features of the HSS framework, such as the distinction between non-verbal motion situations and their linguistic construals.
Reading America, Reading Rodriguez: Exploring American Literature at an English Prison Book Group
Authors: METCALF JOSEPHINE , SKINNER LAURA
Published: February 2024

This article details a cutting-edge Knowledge Exchange initiative which advanced the ongoing partnership between the University of Hull and HMP Hull, and stemmed from the annual BAAS conference, held in Hull in April 2022. The purpose of the article is to explore the value of critiquing US culture in a nonacademic setting and the extent to which a prison reading group presents a productive opportunity for so doing. Our research analyses the reception of a number of texts discussed in an American-themed book club hosted in HMP Hull, with a particular focus upon the responses of prison learners to the literary works of gang-member-turned-best-selling-author Luis J. Rodriguez.
Transformation of a plasma boundary curvature into electrical impulses moving along a plasma surface
Authors: Gradov O.M.
Published: February 2024

The self-consistent propagation of electrical impulses and of the accompanying distortions of the electron surface in the framework of a cold plasma model with a sharp boundary has been described with help of a derived system of two equations. The method of ‘shallow water theory’ has been applied for the case of bounded plasma and deriving an equation with which to link the spatial and temporal structures and evolution of the boundary curvature and the surface charge. Under certain conditions, such perturbations can propagate along the boundary without changing their shape for a long distance. An approximate analytical solution has been found, and numerical calculations have been performed. Mutual connections between basic parameters of the considered perturbations (velocity components, electrostatic field, etc.) have been presented.
Sex differences in growth and neurocognitive development in infancy and early childhood
Authors: Moore Sophie E.
Published: February 2024

Undernutrition in early life remains a significant public health challenge affecting millions of infants and young children globally. Children who are wasted, stunted or underweight are at increased risk of morbidity and mortality. Undernutrition at critical periods also impacts on aspects of neurodevelopment, with longer-term consequences to educational performance and mental health outcomes. Despite consistent evidence highlighting an increased risk of neonatal and infant mortality among boys, a common assumption held across many disciplines is that girls are more vulnerable with respect to early-life exposures. In relation to undernutrition, however, recent evidence indicates the reverse, and in contexts of food insecurity, boys are at increased risk of undernutrition in early life compared to girls, with sex-specific risks for neurodevelopmental deficits. These effects appear independent of social factors that may favour boys, such as gender disparities in infant feeding practices and health-seeking behaviours. The observed vulnerability among boys may therefore be underpinned by biological processes such as differential energy requirements during periods of rapid growth. As boys have greater needs for growth and maintenance, then, in times of nutritional hardship, these needs may not be met resulting in risk of undernutrition and subsequent health consequences. In view of this emerging evidence, a greater understanding of the mechanisms behind this vulnerability among boys is needed and policy considerations to protect boys should be considered. This review will explore sex differences in risk of undernutrition and consider these in the context of existing programmes and policies.
Employee Experiences Providing Nutritional Care during the COVID-19 Pandemic
Authors: Alford Heather , Hunter Paulette , Cammer Allison
Published: February 2024

Nutritional care is a critical, yet often overlooked component of quality care in long-term care (LTC) that is linked to culture, socialization, and residents’ psychological and physiological well-being. Given that several COVID-19 infection control protocols affected nutritional care, this study aimed to understand employees’ experiences of these changes. Seven semi-structured interviews were conducted with Saskatchewan healthcare employees from several disciplines, all of whom had a role in supporting nutritional care in LTC. The resulting interview transcripts were analyzed using reflexive thematic analysis. Three main themes characterized the interviewees’ reflections: regression to an institutional mealtime environment, unrealistic expectations, and concern for residents. Given the centrality of nutritional care to quality of life, strategies tailored to support staff in providing relationship-centered nutritional care must be further articulated to maintain standards of care for LTC residents in future outbreaks and epidemics.
Percutaneous closure of patent ductus arteriosus versus surgical treatment in low-birth-weight preterms: a systematic review and meta-analysis
Authors: Melchior Camila Dos Santos , Neves Gabriel Rezende , de Oliveira Bruna Letícia , Toguchi Ana Carolina , Lopes Jesana Costa , Pavione Marcos Alves , Enríquez Shirley Katherine Tinajero
Published: February 2024

Introduction:

The optimal management of a patent ductus arteriosus in a population of preterm infants is controversial. Traditionally, when the patent ductus arteriosus does not close either with conservative treatment or in response to pharmacological therapy, the only option is surgical closure. However, transcatheter occlusion might provide a therapeutic alternative.

Methods:

We searched PubMed, Embase, and Cochrane databases for non-randomised and randomised controlled trials that compared transcatheter percutaneous closure of patent ductus arteriosus with surgical ligation in low-birth-weight preterm infants (<2,500 g). A random-effects model was used for outcomes with high heterogeneity.

Results:

We included twelve studies comprising 4,668 low-birth-weight preterm infants, of whom 966 (20.7%) were in the transcatheter percutaneous closure group, and 3,702 (79.3%) patients were included in the surgical group. All-cause mortality (OR 0.28; 95% confidence interval 0.18–0.423; p < 0.00001; I2 = 0%) and haemodynamic instability (OR 0.10; 95% confidence interval 0.05–0.21; p < 0.001; I2 = 14%) were significantly lower in the transcatheter percutaneous closure group. There was no significant difference between transcatheter and surgical patent ductus arteriosus closure for the outcomes of bronchopulmonary dysplasia (0.93; 95% confidence interval 0.46–1.87; p = 0.83; I2 = 0%) and major complications (OR 0.76; 95% confidence interval 0.34–1.69; p = 0.51; I2 = 43%).

Conclusion:

These findings suggest that transcatheter patent ductus arteriosus closure in preterm infants under 2,500 g is a safe and effective alternative to surgical treatment. There was a substantial reduction in all-cause mortality and haemodynamic instability with transcatheter intervention compared to surgical closure.
The relationship between peritraumatic distress, mental health symptoms, and functioning impairment in healthcare workers during the COVID-19 emergency
Authors: Carmassi Claudia , Bertelloni Carlo Antonio , Dell’Oste Valerio , Pedrinelli Virginia , Fantasia Sara , Atti Anna-Rita , Goracci Arianna , Nanni Maria Giulia , Massimetti Enrico , Dell’Osso Lilliana , Bui Eric
Published: February 2024

Objective

Healthcare workers (HCWs) were considered a population at risk for developing psychiatric symptoms during the COVID-19 pandemic, such as anxiety, depression, and post-traumatic stress disorder (PTSD). Peritraumatic distress is associated with post-traumatic psychopathological symptoms; however, little is known about how it may affect functioning. The study aimed at evaluating the level of peritraumatic distress in a sample of HCWs during the first wave of the COVID-19 pandemic and at examining the relationship between peritraumatic distress, mental health symptoms, and functioning impairment.

Methods

A sample of 554 frontline HCWs were consecutively enrolled in major university hospitals and community services in Italy. The PDI, IES-R, PHQ-9, and GAD-7 were used to assess peritraumatic distress, symptoms of PTSD, depression, and anxiety, respectively, and the WSAS to investigate functioning impairment. PDI scores were higher among females, community services, physicians, and nurses. Furthermore, the PDI correlated significantly with the GAD-7, PHQ-9, IES-R, and WSAS.

Results

In a mediation analysis, the direct effect of PDI on WSAS and the indirect effects through the PHQ-9 and IES-R were statistically significant (P < .001).

Conclusion

Peritraumatic distress reported by HCWs was associated with symptoms of PTSD, depression, and anxiety, but the association with reduced functioning may be only partially mediated through symptoms of depression and PTSD.
Astrobiocentrism: reflections on challenges in the transition to a vision of life and humanity in space
Authors: Chon-Torres Octavio Alfonso , Chela-Flores Julian , Dunér David , Persson Erik , Milligan Tony , Martínez-Frías Jesús , Losch Andreas , Pryor Adam , Murga-Moreno César Andreé
Published: February 2024

Astrobiocentrism is a vision that places us in a scenario of confirmation of life in the universe, either as a second genesis or as an expansion of humanity in space. It manages to raise consistent arguments in relation to questions such as what would happen to knowledge if life were confirmed in the universe, how would this change the way we understand our place in the cosmos? Astrobiocentrism raises a series of reflections in the context of confirmed discovery, and it develops concepts that work directly with what would happen after irrefutable evidence has been obtained that we are not alone in space. Unlike biocentrism or ecocentrism, the astrobiocentric view is not limited to the Earth-centric perspective, and for it incorporates a multi-, inter- and transdisciplinary understanding. Therefore, the aim of this paper is to make a reflection on the astrobiocentric issues related to the challenges and problems of the discovery of life in the universe and the expansion of mankind into space. Here we explore some aspects of the transition from biogeocentrism to astrobiocentrism, astrobiosemiotics,homo mensura, moral community, planetary sustainability and astrotheology.
Translation and linguistic validation of the Sheffield Profile for Assessment and Referral for Care (SPARC) to Colombian Spanish
Authors: Moreno Socorro , Mendieta Cindy V. , de Vries Esther , Ahmedzai Sam H. , Rivera Karen , Cortes-Mora Camilo , Calvache Jose A.
Published: February 2024

Objectives

We aimed to translate and linguistically and cross-culturally validate Sheffield Profile for Assessment and Referral for Care (SPARC) in Spanish for Colombia (SPARC-Sp).

Methods

The linguistic validation of SPARC followed a standard methodology. We conducted focus groups to assess the comprehensibility and feasibility. The acceptability was assessed using a survey study with potential users.

Results

The comprehensibility assessment showed that additional adjustments to those made during the translation-back-translation process were required to apply SPARC-Sp in rural and low-schooled populations. It also identified the need for alternative administration mechanisms for illiterate people. The acceptability survey showed that potential users found SPARC-Sp as not only acceptable but also highly desirable. However, they desired to expand the number of items in all domains.

Significance of results

Beyond the semantic and conceptual validity attained through the back-translation process, actual cultural validity could be acquired thanks to the comprehensibility tests. Although extending the instrument is something potential users would like to do, it would make it less feasible to utilize the SPARC-Sp in clinical settings. Nonetheless, the instrument might benefit from the inclusion of a domain that evaluates challenges encountered when accessing the health-care system. For communities lacking literacy, alternate administration methods must also be considered.
A broadband circularly polarized antenna for millimeter-wave applications
Authors: Xie Yue , Zhao Jianping , Sun Yurong , Xu Juan
Published: February 2024

This paper presents a broadband circularly polarized (CP) antenna array for millimeter-wave applications, and the antenna array has the advantages of wide impedance bandwidth (IBW), novel CP design, and low profile. The antenna unit consists of a two-layer substrate and two pairs of magnetoelectric dipoles. Stepped microstrip lines coupled by rectangular slits form a feeder network for easy integration. The axial ratio bandwidth (ARBW) is extended because a pair of parasitic patches is loaded and an elliptical perturbation is added. The simulation results show that the antenna has an ARBW of 18.6% (26.4–31.9 GHz) and an IBW of 45.5% (20.6–32.7 GHz), with a gain greater than 7.11 dBic in the IBW. To improve the gain of the antenna, a 2 × 2 antenna array is designed, fabricated, and measured. The measured results show that the array has an ARBW of 16.6% (26.42–31.21 GHz), an IBW of 41.6% (22.28–33.97 GHz), a peak gain of 13.89 dBic in the IBW, the cross-polarization levels in the xoz-plane and yoz-plane are above 20 dB, and a radiation efficiency greater than 89%.
Delayed Onset of Indian Summer Monsoon in Response to CO2 Removal
Authors: Zhang Suqin , Qu Xia , Huang Gang , Hu Peng , Zhou Shijie , Wu Liang
Published: February 2024

Understanding the response of the Indian summer monsoon (ISM) onset to CO2 forcing is of utmost importance for rain‐fed agriculture and water management. In this study, we utilized an idealized symmetric CO2 removal scenario from the sixth phase of the Coupled Model Intercomparison Project to analyze the reversibility of monsoon onset. The results show that ISM onset is reversible but exhibits strong asymmetry: it undergoes minimal changes during the ramp‐up phase, but experiences rapid postponement as the CO2 begins to decline; Eventually, it is delayed more than 1 week when the CO2 concentration is restored to the initial level. To investigate the possible underlying mechanisms, we decomposed the climate response to CO2 forcing into the fast and slow processes. Notably, it is the enhanced slow response, which is driven by long‐term sea surface temperature (SST) changes, that dominates the asymmetric response of ISM onset. This slow response delays the ISM onset by strengthening near‐surface poleward land‐sea moist static energy contrast, thereby weakening the lower‐tropospheric monsoonal circulation. Based on the atmospheric component model simulations, we found that both the uniform SST change and patterned SST changes in the slow response contribute to the delay of ISM onset, but the latter plays a dominant role. Our results emphasize the importance of thoroughly assessing regional hydrological cycle features when designing the CO2 removal pathways.
On the Importance of a Geostationary View for Tropical Cloud Feedback
Authors: Lee Yoon‐Kyoung , Choi Yong‐Sang , Hwang Jiwon , Hu Xiaoming , Yang Song
Published: February 2024

This study shows that geostationary satellites are critical to estimate the accurate cloud feedback strength over the tropical western Pacific (TWP). Cloud feedback strength was calculated by the simultaneous relation between cloud cover and sea surface temperature (SST) over the TWP [120°E–170°E, 20°S–20°N]. During 2011–2018, the cloud cover was obtained by geostationary earth orbit satellite (GEO) and low‐level earth orbit satellite (LEO) (AGEO, ALEO), and the NOAA's all‐sky SST (To) was weighted with the clear‐sky fraction observed by GEO and LEO (TwGEO; TwLEO). The linear regression coefficients between clouds and SST are very different: −7.93%K−1 (AGEO/TwGEO), −6.94%K−1 (ALEO/TwGEO), −1.35%K−1 (AGEO/TwLEO), −0.69%K−1 (ALEO/TwLEO), −0.02 %K−1 (AGEO/To), and −0.50 %K−1 (ALEO/To). Among these, only the TwGEO values provided a valid cloud feedback signal. This is because GEO's field of view is large enough to simultaneously capture cloud cover over the entire TWP.
Active Shortening Simultaneous to Normal Faulting Based on GNSS, Geophysical, and Geological Data: The Seismogenic Ventas de Zafarraya Fault (Betic Cordillera, Southern Spain)
Authors: Madarieta‐Txurruka Asier , González‐Castillo Lourdes , Peláez José A. , Galindo‐Zaldívar Jesús , Borque María J. , Lacy María C. , Ruiz‐Armenteros Antonio M. , Henares Jesús , Ruano Patricia , Sánchez‐Alzola Alberto , Avilés Manuel , Rodríguez‐Caderot Gracia , Martínez‐Moreno Francisco José , Tendero‐Salmerón Víctor , Vinardell‐Peña Raquel , Gil Antonio J.
Published: February 2024

The central Betic Cordillera, southern Spain, is affected by an uplift related to the NNW–SSE Eurasia‐Nubia convergence and shallow ENE–WSW orthogonal extension accommodated by the extensional system of the Granada Basin. The combination of geophysical, geodetic, and geological data reveals that the southwestern boundary of this extensional system is a seismically active compressional front extending from the W to the SW of the Granada Basin. The near‐field Global Navigation Satellite System data determine NNE–SSW shortening of up to 2 mm/yr of the compressional front in the Zafarraya Polje. In this setting, the normal Ventas de Zafarraya Fault developed as a result of the bending‐moment extension of the Sierra de Alhama antiform and was last reactivated during the 1884 Andalusian earthquake (Mw 6.5). The uplift in the central Betic Cordillera together with the subsidence in the Western Alborán Basin may facilitate a westward to southwestward gravitational collapse through the extensional detachment of the Granada Basin. The heterogeneous crust of the Betic Cordillera would generate the compressional front, which is divided into two sectors: thrusting to the west, and folding associated with buttressing to the south. Our results evidence that basal detachments, linking extensional fault activity with compressional fronts, may determine the activity of local surface structures and the geological hazard in densely populated regions.
Structural insights into histone exchange by human SRCAP complex
Authors: Yu Jiali , Sui Fengrui , Gu Feng , Li Wanjun , Yu Zishuo , Wang Qianmin , He Shuang , Wang Li , Xu Yanhui
Published: February 2024

Histone variant H2A.Z is found at promoters and regulates transcription. The ATP-dependent chromatin remodeler SRCAP complex (SRCAP-C) promotes the replacement of canonical histone H2A–H2B dimer with H2A.Z–H2B dimer. Here, we determined structures of human SRCAP-C bound to H2A-containing nucleosome at near-atomic resolution. The SRCAP subunit integrates a 6-subunit actin-related protein (ARP) module and an ATPase-containing motor module. The ATPase-associated ARP module encircles half of the nucleosome along the DNA and may restrain net DNA translocation, a unique feature of SRCAP-C. The motor module adopts distinct nucleosome binding modes in the apo (nucleotide-free), ADP-bound, and ADP-BeFx-bound states, suggesting that ATPase-driven movement destabilizes H2A–H2B by unwrapping the entry DNA and pulls H2A–H2B out of nucleosome through the ZNHIT1 subunit. Structure-guided chromatin immunoprecipitation sequencing analysis confirmed the requirement of H2A-contacting ZNHIT1 in maintaining H2A.Z occupancy on the genome. Our study provides structural insights into the mechanism of H2A-H2A.Z exchange mediated by SRCAP-C.
Enhanced antifouling and separation capabilities of polydopamine@Ce-MOF functionalized PES ultrafiltration membrane
Authors: Kadadou Dana , Arumugham Thanigaivelan , Tizani Lina , Hasan Shadi W.
Published: February 2024

The need for antifouling membranes is substantial due to fouling’s negative impact on the effectiveness and durability of ultrafiltration membranes. In recent times, Metal-Organic Frameworks (MOFs) have emerged as effective additives for crafting ultrafiltration (UF) membranes. However, to harness their benefits and mitigate their drawbacks, a well-considered approach is imperative. In this work, polyethersulfone (PES) UF membranes were tailored with polydopamine (PDA)-modified cerium (Ce)-MOF (PDA@Ce-MOF) to achieve satisfactory antifouling properties and to remove biological macromolecules such as bovine serum albumin (BSA) and humic acid (HA) from wastewater. The non-solvent induced phase separation (NIPS) method was used to simultaneously fabricate the membrane and modify it with different PDA@Ce-MOF concentrations ranging from 0.05 to 0.50 wt.%. Results showed significant improvements on the membrane’s morphology, hydrophilicity, porosity and pore size at 0.10 wt.% loading of PDA@Ce-MOF. The synergistic effect of the PDA and Ce-MOF on the membrane improved the pure water flux (337 L m−2 h−1) and filtration performance in the filtration of Bovine serum albumin (BSA) and humic acid (HA) reporting 145 L m−2 h−1 and 98% rejection, and 164 L m−2 h−1 and 88% rejection; respectively. Further, water-attracting functional moieties of PDA@Ce-MOF hindered the deposition of BSA protein on the membrane surface, resulting in an excellent flux recovery ratio (FRR) of ~87%, alongside mitigated irreversible fouling.
Longitudinal changes in brain metabolites following pediatric concussion
Authors: La Parker L. , Walker Robyn , Bell Tiffany K. , Craig William , Doan Quynh , Beauchamp Miriam H. , Zemek Roger , Yeates Keith Owen , Harris Ashley D. ,   Pediatric Emergency Research Canada A-CAP study team, Yeates Keith Owen , Beauchamp Miriam H. , Bjornson Bruce H. , Gravel Jocelyn , Mikrogianakis Angelo , Goodyear Bradley , Abdeen Nishard , Beaulieu Christian , Dehaes Mathieu , Deschenes Sylvain , Harris Ashley D. , Lebel Catherine , Lamont Ryan , Williamson Tyler , Barlow Karen Maria , Bernier Francois , Brooks Brian L. , Emery Carolyn , Freedman Stephen B. , Kowalski Kristina , Mrklas Kelly , Tomfohr-Madsen Lianne , Schneider Kathryn J.
Published: February 2024

Concussion is commonly characterized by a cascade of neurometabolic changes following injury. Magnetic Resonance Spectroscopy (MRS) can be used to quantify neurometabolites non-invasively. Longitudinal changes in neurometabolites have rarely been studied in pediatric concussion, and fewer studies consider symptoms. This study examines longitudinal changes of neurometabolites in pediatric concussion and associations between neurometabolites and symptom burden. Participants who presented with concussion or orthopedic injury (OI, comparison group) were recruited. The first timepoint for MRS data collection was at a mean of 12 days post-injury (n = 545). Participants were then randomized to 3 (n = 243) or 6 (n = 215) months for MRS follow-up. Parents completed symptom questionnaires to quantify somatic and cognitive symptoms at multiple timepoints following injury. There were no significant changes in neurometabolites over time in the concussion group and neurometabolite trajectories did not differ between asymptomatic concussion, symptomatic concussion, and OI groups. Cross-sectionally, Choline was significantly lower in those with persistent somatic symptoms compared to OI controls at 3 months post-injury. Lower Choline was also significantly associated with higher somatic symptoms. Although overall neurometabolites do not change over time, choline differences that appear at 3 months and is related to somatic symptoms.
Temporal variations in the gut microbial diversity in response to high-fat diet and exercise
Authors: Imdad Saba , So Byunghun , Jang Junho , Park Jinhan , Lee Sam-Jun , Kim Jin-Hee , Kang Chounghun
Published: February 2024

High-fat diet-induced obesity is a pandemic caused by an inactive lifestyle and increased consumption of Western diets and is a major risk factor for diabetes and cardiovascular diseases. In contrast, exercise can positively influence gut microbial diversity and is linked to a decreased inflammatory state. To understand the gut microbial variations associated with exercise and high-fat diet over time, we conducted a longitudinal study to examine the effect of covariates on gut microbial diversity and composition. Young mice were divided into four groups: Chow-diet (CHD), high-fat diet (HFD), high-fat diet + exercise (HFX), and exercise only (EXE) and underwent experimental intervention for 12 weeks. Fecal samples at week 0 and 12 were collected for DNA extraction, followed by 16S library preparation and sequencing. Data were analyzed using QIIME 2, R and MicrobiomeAnalyst. The Bacteroidetes-to-Firmicutes ratio decreased fivefold in the HFD and HFX groups compared to that in the CHD and EXE groups and increased in the EXE group over time. Alpha diversity was significantly increased in the EXE group longitudinally (p < 0.02), whereas diversity (Shannon, Faith’s PD, and Fisher) and richness (ACE) was significantly reduced in the HFD (p < 0.005) and HFX (p < 0.03) groups over time. Beta diversity, based on the Jaccard, Bray–Curtis, and unweighted UniFrac distance metrics, was significant among the groups. Prevotella, Paraprevotella, Candidatus arthromitus, Lactobacillus salivarius, L. reuteri, Roseburia, Bacteroides uniformis, Sutterella, and Corynebacterium were differentially abundant in the chow-diet groups (CHD and EXE). Exercise significantly reduced the proportion of taxa characteristic of a high-fat diet, including Butyricimonas, Ruminococcus gnavus, and Mucispirillum schaedleri. Diet, age, and exercise significantly contributed to explaining the bacterial community structure and diversity in the gut microbiota. Modulating the gut microbiota and maintaining its stability can lead to targeted microbiome therapies to manage chronic and recurrent diseases and infections.
A novel anoikis-related gene signature identifies LYPD1 as a novel therapy target for bladder cancer
Authors: Song Zhen , Gui Shikai , Xiao Shuaiyun , Rao Xuepeng , Cong Na , Deng Huanhuan , Yu Zhaojun , Zeng Tao
Published: February 2024

Bladder cancer (BLCA) is a malignant tumor associated with unfavorable outcomes. Studies suggest that anoikis plays a crucial role in tumor progression and cancer cell metastasis. However, its specific role in bladder cancer remains poorly understood. Our objective was to identify anoikis-related genes (ARGs) and subsequently construct a risk model to assess their potential for predicting the prognosis of bladder cancer.The transcriptome data and clinical data of BLCA patients were sourced from The Cancer Genome Atlas and GEO database. We then performed the differential expression analysis to screen differentially expressed ARGs. Subsequently, we conducted non-negative matrix factorization (NMF) clustering analysis to establish molecular subtypes based on the differentially expressed ARGs. The CIBERSORT algorithm was used to estimate the quantification of different cell infiltration in BLCA tumor microenviroment. A prognostic risk model containing 7 ARGs was established using Lasso-Cox regression analysis. The nomogram was built for predicting the survival probability of BLCA patients. To determine the drug sensitivity of each sample from the high- and low-risk groups, the R package “pRRophetic” was performed. Finally, the role of LYPD1 was explored in BLCA cell lines.We identified 90 differential expression ARGs and NMF clustering categorizated the BLCA patientss into two distinct groups (cluster A and B). Patients in cluster A had a better prognosis than those in cluster B. Then, we established a ARGs risk model including CALR, FASN, FOSL1, JUN, LYPD1, MST1R, and SATB1, which was validated in the train and test set. The results suggested overall survival rate was much higher in low risk group than high risk group. The cox regression analysis, ROC curve analysis, and nomogram collectively demonstrated that the risk model served as an independent prognostic factor. The high risk group had a higher level TME scores compared to the low risk group. Furthermore, LYPD1 was low expression in BLCA cells and overexpression of LYPD1 inhibits the prolifearation, migration and invasion.In the current study, we have identified differential expression ARGs and constructed a risk model with the promise for guiding prognostic predictions and provided a therapeutic target for patients with BLCA.
Cluster-based histopathology phenotype representation learning by self-supervised multi-class-token hierarchical ViT
Authors: Ye Jiarong , Kalra Shivam , Miri Mohammad Saleh
Published: February 2024

Developing a clinical AI model necessitates a significant amount of highly curated and carefully annotated dataset by multiple medical experts, which results in increased development time and costs. Self-supervised learning (SSL) is a method that enables AI models to leverage unlabelled data to acquire domain-specific background knowledge that can enhance their performance on various downstream tasks. In this work, we introduce CypherViT, a cluster-based histo-pathology phenotype representation learning by self-supervised multi-class-token hierarchical Vision Transformer (ViT). CypherViT is a novel backbone that can be integrated into a SSL pipeline, accommodating both coarse and fine-grained feature learning for histopathological images via a hierarchical feature agglomerative attention module with multiple classification (cls) tokens in ViT. Our qualitative analysis showcases that our approach successfully learns semantically meaningful regions of interest that align with morphological phenotypes. To validate the model, we utilize the DINO self-supervised learning (SSL) framework to train CypherViT on a substantial dataset of unlabeled breast cancer histopathological images. This trained model proves to be a generalizable and robust feature extractor for colorectal cancer images. Notably, our model demonstrates promising performance in patch-level tissue phenotyping tasks across four public datasets. The results from our quantitative experiments highlight significant advantages over existing state-of-the-art SSL models and traditional transfer learning methods, such as those relying on ImageNet pre-training.
Mogrol stimulates G-protein-coupled bile acid receptor 1 (GPBAR1/TGR5) and insulin secretion from pancreatic β-cells and alleviates hyperglycemia in mice
Authors: Tanaka Chisato , Harada Naoki , Teraoka Yoshiaki , Urushizaki Hiroki , Shinmori Yoh , Onishi Teruaki , Yotsumoto Yusuke , Ito Yuta , Kitakaze Tomoya , Inui Takashi , Murata Yuji , Inui Hiroshi , Yamaji Ryoichi
Published: February 2024

Target identification is a crucial step in elucidating the mechanisms by which functional food components exert their functions. Here, we identified the G-protein-coupled bile acid receptor 1 (GPBAR1, also known as TGR5) as a target of the triterpenoid mogrol, a class of aglycone mogroside derivative from Siraitia grosvenorii. Mogrol, but not mogrosides, activated cAMP-response element-mediated transcription in a TGR5-dependent manner. Additionally, mogrol selectively activated TGR5 but not the other bile acid-responsive receptors (i.e., farnesoid X receptor, vitamin D receptor, or muscarinic acetylcholine receptor M3). Several amino acids in TGR5 (L71A2.60, W75AECL1, Q77AECL1, R80AECL1, Y89A3.29, F161AECL2, L166A5.39, Y240A6.51, S247A6.58, Y251A6.62, L262A7.35, and L266A7.39) were found to be important for mogrol-induced activation. Mogrol activated insulin secretion under low-glucose conditions in INS-1 pancreatic β-cells, which can be inhibited by a TGR5 inhibitor. Similar effects of mogrol on insulin secretion were observed in the isolated mouse islets. Mogrol administration partially but significantly alleviated hyperglycemia in KKAy diabetic mice by increasing the insulin levels without affecting the β-cell mass or pancreatic insulin content. These results suggest that mogrol stimulates insulin secretion and alleviates hyperglycemia by acting as a TGR5 agonist.
Assessment of adverse events among healthcare workers following the Janssen COVID-19 vaccine in Tigray, Ethiopia
Authors: Abera Bisrat Tesfay , Teka Hale , Berhe Ephrem , Gebru Marta Abrha , Zenebe Dawit , Abraha Hiluf Ebuy , Hailu Abraha
Published: February 2024

Apart from the inequality in vaccination, war zones and areas where communication is disrupted are affected by myths and misconceptions about COVID-19 vaccines, heightening vaccine hesitancy. Local data on adverse events of the vaccines and their mildness can increase confidence and acceptance of the vaccines in the respective population. In areas of conflict and communication blackouts, the perception of the vaccines by health workers is of paramount importance as public health recommendations may not reach the public. Therefore, the scientific evaluation of adverse events following COVID-19 vaccination in such areas is invaluable. This cross-sectional, facility-based study was conducted using a structured, interviewer-administered questionnaire to assess the adverse events experienced by healthcare workers who received the Janssen COVID-19 vaccine. The sample was divided proportionally to the number of vaccinated healthcare workers for the different healthcare professions, and participants were then randomly selected from each profession. Prior to data collection, a pilot test was conducted with 5% of the sample size outside the selected hospital. The study was conducted using a structured questionnaire completed by an interviewer to assess adverse events in 442 healthcare workers who had received the Janssen COVID-19 vaccine between July 11 and 25, 2022. The study period was from August 15 to September 15, 2022. A significant number of healthcare workers [366 (83.3%); 95% CI 79.5%, 86.5%] experienced at least one adverse event. Nearly 90% of participants reported that the adverse events were mild to moderate. Pain at the injection site [307 (69.5%); 95% CI 65.0%, 73.6%] and headache [247 (55.9%); 95% CI 51.2%, 60.4%] were the most common local and systemic adverse events, respectively. Two HCWs experienced anaphylactic reaction. Younger age was significantly associated with the occurrence of adverse events. We deciphered that the adverse events reported by the study participants were not different from the typically occurring vaccine-related adverse reactions, and therefore concluded that post-vaccination reactions in healthcare workers were minor. Although vaccination in Tigray is currently stalled due to the siege, responsible stakeholders should develop a mechanism to track population-wide adverse events once the vaccines start to rollout.
The Ykt6–Snap29–Syx13 SNARE complex promotes crinophagy via secretory granule fusion with Lamp1 carrier vesicles
Authors: Szenci Győző , Glatz Gábor , Takáts Szabolcs , Juhász Gábor
Published: February 2024

In the Drosophila larval salivary gland, developmentally programmed fusions between lysosomes and secretory granules (SGs) and their subsequent acidification promote the maturation of SGs that are secreted shortly before puparium formation. Subsequently, ongoing fusions between non-secreted SGs and lysosomes give rise to degradative crinosomes, where the superfluous secretory material is degraded. Lysosomal fusions control both the quality and quantity of SGs, however, its molecular mechanism is incompletely characterized. Here we identify the R-SNARE Ykt6 as a novel regulator of crinosome formation, but not the acidification of maturing SGs. We show that Ykt6 localizes to Lamp1+ carrier vesicles, and forms a SNARE complex with Syntaxin 13 and Snap29 to mediate fusion with SGs. These Lamp1 carriers represent a distinct vesicle population that are functionally different from canonical Arl8+, Cathepsin L+ lysosomes, which also fuse with maturing SGs but are controlled by another SNARE complex composed of Syntaxin 13, Snap29 and Vamp7. Ykt6- and Vamp7-mediated vesicle fusions also determine the fate of SGs, as loss of either of these SNAREs prevents crinosomes from acquiring endosomal PI3P. Our results highlight that fusion events between SGs and different lysosome-related vesicle populations are critical for fine regulation of the maturation and crinophagic degradation of SGs.
Terbium-149 production: a focus on yield and quality improvement towards preclinical application
Authors: Favaretto C. , Grundler P. V. , Talip Z. , Köster U. , Johnston K. , Busslinger S. D. , Sprung P. , Hillhouse C. C. , Eichler R. , Schibli R. , Müller C. , van der Meulen N. P.
Published: February 2024

Terbium-149 (T1/2 = 4.1 h, Eα = 3.98 MeV (16.7%), 28 µm range in tissue) is a radionuclide with potential for targeted alpha therapy. Due to the negligible emission of α-emitting daughter nuclides, toxicity to healthy tissue may be reduced in comparison with other α-particle emitters. In this study, terbium-149 was produced via 1.4 GeV proton irradiation of a tantalum target at the CERN-ISOLDE facility. The spallation products were mass separated and implanted on zinc-coated foils and, later, radiochemically processed. Terbium-149 was separated from the co-produced isobaric radioisotopes and the zinc coating from the implantation foil, using cation-exchange and extraction chromatographic techniques, respectively. At the end of separation, up to 260 MBq terbium-149 were obtained with > 99% radionuclidic purity. Radiolabeling experiments were performed with DOTATATE, achieving 50 MBq/nmol apparent molar activity with radiochemical purity > 99%. The chemical purity was determined by inductively coupled plasma–mass spectrometry measurements, which showed lead, copper, iron and zinc only at ppb level. The radiolabeling of the somatostatin analogue DOTATATE with [149Tb]TbCl3 and the subsequent in vivo PET/CT scans conducted in xenografted mice, showing good tumor uptake, further demonstrated product quality and its ability to be used in a preclinical setting.
Neural correlates of transfer of learning in motor coordination tasks: role of inhibitory and excitatory neurometabolites
Authors: Rasooli Amirhossein , Chalavi Sima , Li Hong , Seer Caroline , Adab Hamed Zivari , Mantini Dante , Sunaert Stefan , Mikkelsen Mark , Edden Richard A. E. , Swinnen Stephan P.
Published: February 2024

We aimed to investigate transfer of learning, whereby previously acquired skills impact new task learning. While it has been debated whether such transfer may yield positive, negative, or no effects on performance, very little is known about the underlying neural mechanisms, especially concerning the role of inhibitory (GABA) and excitatory (Glu) (measured as Glu + glutamine (Glx)) neurometabolites, as measured by magnetic resonance spectroscopy (MRS). Participants practiced a bimanual coordination task across four days. The Experimental group trained a task variant with the right hand moving faster than the left (Task A) for three days and then switched to the opposite variant (Task B) on Day4. The control group trained Task B across four days. MRS data were collected before, during, and after task performance on Day4 in the somatosensory (S1) and visual (MT/V5) cortex. Results showed that both groups improved performance consistently across three days. On Day4, the Experimental group experienced performance decline due to negative task transfer while the control group continuously improved. GABA and Glx concentrations obtained during task performance showed no significant group-level changes. However, individual Glx levels during task performance correlated with better (less negative) transfer performance. These findings provide a first window into the neurochemical mechanisms underlying task transfer.
The effect of macroscopic herd inputs on individual investment behaviour
Authors: Nielsen Kristian Roed , Kaiser Micha , Glückstad Fumiko Kano
Published: February 2024

Decisions are rarely made in isolation and the role of others’ decisions in guiding our own has been observed in a diversity of contexts. This influence is often argued to result from an information cascade, where decisions in a sequential setting are influenced by the early decisions of others. However, the degree to which individuals modify behaviour through the integration of social information (i.e., other people’s decisions) varies considerably. While significant literature has been dedicated to understanding individual determinants for this variation, we propose that we should not ignore the aggregate characteristics of the herd itself. Specifically, we examine whether the scale and longevity of the herd itself at the time when an individual decides, defined as macroscopic herd inputs, influence whether individuals integrate social information. By employing data from a social trading platform, we find that macroscopic herd inputs exert a strong influence on individual investment decisions, showing that the influence of others’ behaviour on our own is in part dependent on the nature of the herd itself.
A common trajectory of gut microbiome development during the first month in healthy neonates with limited inter-individual environmental variations
Authors: Wang Xing-An , Li Ju-Pi , Lee Maw-Sheng , Yang Shun-Fa , Chang Yi-Sheng , Chen Ling , Li Chang-Wei , Chao Yu-Hua
Published: February 2024

The early development of the gut microbiome is governed by multiple factors and has significantly long-term effects on later-in-life health. To minimize inter-individual variations in the environment, we determined developmental trajectories of the gut microbiome in 28 healthy neonates during their stay at a postpartum center. Stool samples were collected at three time points: the first-pass meconium within 24 h of life, and at 7 and 28 days of age. Illumina sequencing of the V3–V4 region of 16S rRNA was used to investigate microbiota profiles. We found that there was a distinct microbiota structure at each time point, with a significant shift during the first week. Proteobacteria was most abundant in the first-pass meconium; Firmicutes and Actinobacteria increased with age and were substituted as the major components. Except for a short-term influence of different delivery modes on the microbiota composition, early microbiome development was not remarkably affected by gravidity, maternal intrapartum antibiotic treatment, premature rupture of membranes, or postnatal phototherapy. Hence, our data showed a similar developmental trajectory of the gut microbiome during the first month in healthy neonates when limited in environmental variations. Environmental factors external to the host were crucial in the early microbiome development.
Digital interventions to promote psychological resilience: a systematic review and meta-analysis
Authors: Schäfer Sarah K. , von Boros Lisa , Schaubruch Lea M. , Kunzler Angela M. , Lindner Saskia , Koehler Friederike , Werner Tabea , Zappalà Federico , Helmreich Isabella , Wessa Michèle , Lieb Klaus , Tüscher Oliver
Published: February 2024

Societies are exposed to major challenges at an increasing pace. This underscores the need for preventive measures such as resilience promotion that should be available in time and without access barriers. Our systematic review summarizes evidence on digital resilience interventions, which have the potential to meet these demands. We searched five databases for randomized-controlled trials in non-clinical adult populations. Primary outcomes were mental distress, positive mental health, and resilience factors. Multilevel meta-analyses were performed to compare intervention and control groups at post-intervention and follow-up assessments. We identified 101 studies comprising 20,010 participants. Meta-analyses showed small favorable effects on mental distress, SMD = –0.24, 95% CI [–0.31, –0.18], positive mental health, SMD = 0.27, 95% CI [0.13, 0.40], and resilience factors, SMD = 0.31, 95% CI [0.21, 0.41]. Among middle-aged samples, older age was associated with more beneficial effects at follow-up, and effects were smaller for active control groups. Effects were comparable to those of face-to-face interventions and underline the potential of digital resilience interventions to prepare for future challenges.
Efficient healing of diabetic wounds by MSC-EV-7A composite hydrogel via suppression of inflammation and enhancement of angiogenesis
Authors: Long Xinyi , Yuan Qian , Tian Rui , Zhang Wanting , Liu Lang , Yang Minghui , Yuan Xin , Deng Zhujie , Li Quanjiang , Sun Ronghui , Kang Yuyi , Peng Yingying , Kuang Xiubin , Zeng Lingfang , Yuan Zhengqiang
Published: February 2024

An alginate hydrogel loaded with mesenchymal stem-cell-derived extracellular vesicles (EVs) and a 7-amino-acid peptide (7A) constitutes a novel dressing for efficient healing of diabetic wounds with anti-inflammation and pro-angiogenesis mechanisms.
Insights into machine learning models from chemical physics: an energy landscapes approach (EL for ML)
Authors: Niroomand Maximilian P. , Dicks Luke , Pyzer-Knapp Edward O. , Wales David J.
Published: February 2024

In this work, we outline how methods from the energy landscapes field of theoretical chemistry can be applied to study machine learning models. Various applications are found, ranging from interpretability to improved model performance.
Identification of spectral responses of different plastic materials by means of multispectral imaging
Authors: Bragato Giovanni , Piccolo Giovanni , Sattier Gabriele , Sada Cinzia
Published: February 2024

Hyperspectral imaging as a highly innovative solution for detecting, identification and sensing of (micro)plastics, even transparent or randomly dispersed in water.
Experimental determination of the partitioning of representative organic pollutants to the air–water interface
Authors: McLay Emma M. , Abdel Nour Carole , Huang Yao Yan , Golay Zoë M. , Wong-Wah-Chung Pascal , Rossignol Stéphanie , Donaldson D. James
Published: February 2024

Glancing-angle laser-induced fluorescence is used to obtain adsorption isotherms of organic pollutants at the air–water interface.
Combined toxicity and adverse outcome pathways of common pesticides on Chlorella pyrenoidosa
Authors: Zhang Jing , Zhang Jin , Huang Xianhuai , Xie Fazhi , Dai Biya , Ma Tianyi , Zeng Jianping
Published: February 2024

Adverse outcome pathways framework of pesticides.
Biological contributions to biological ion exchange
Authors: Zimmermann Karl , Sampara Pranav , Ziels Ryan , Mohseni Madjid
Published: February 2024

DOC removal by biological ion exchange (BIEX) water filters is mostly via sulphate-based secondary IEX and less through biodegradation mechanisms, which were similar to those in BAC filters, although the two filters had very different microbial community compositions.
Advances in the fabrication of potential nanomaterials for diagnosis and effective treatment of tuberculosis
Authors: El-Shabasy Rehan M. , Zahran Moustafa , Ibrahim Ahmed H. , Maghraby Yasmin R. , Nayel Mohamed
Published: February 2024

This review sheds light on the novel nanomaterials required for rapid and clear diagnosis of tuberculosis, and compiles and discusses their limitations and future prospects.
Bimetallic PdPt alloy nanoparticle-decorated track-etched polyethylene terephthalate membranes for efficient H2 separation
Authors: Saini Nishel , Agarwal Sonalika , Awasthi Kamlendra
Published: February 2024

Bimetallic PdPt alloy nanoparticles of particle size 8 nm, which show high affinity towards H2 gas, were synthesized using a wet chemical synthesis process.