Synthesis and characterization of nanocomposites based on polylactide/silver nanoparticles, obtained by thermochemical reduction of Ag+ ions by natural or synthetic polymers
Authors:   Institute of Macromolecular Chemistry of National Academy of Sciences of Ukraine, Demchenko V. L. , Iurzhenko M. V. ,   Institute of Macromolecular Chemistry of National Academy of Sciences of Ukraine, Kobylinskyi S. M. ,   Institute of Macromolecular Chemistry of National Academy of Sciences of Ukraine, Goncharenko L. A. ,   Institute of Macromolecular Chemistry of National Academy of Sciences of Ukraine
Published: November 2021

The addition of silver ions or nanoparticles to impart antimicrobial properties to polymeric or other materials is a widely used method. However, it should be noted that the antiviral and antimicrobial effect of silver nanoparticles that come into contact with the environment, associated with their size, with a decrease in the size of nanoparticles, their effectiveness increases sharply. In the present work, we used a biodegradable polymer polylactide (PLA), which is obtained by condensation of lactic acid or ring-opening polymerization of lactide. These studies will further contribute to the development of new safe materials, in particular for food packaging, which is undoubtedly an urgent problem. The work aims is to obtain the silver-containing polymer composites based on polylactide by thermochemical reduction of Ag+ ions using natural (chitosan) and synthetic (polyethyleneimine (PEI)) polymers and to study the structure, morphology, thermomechanical and antimicrobial properties of the obtained nanocomposites. Thermochemical reduction of Ag+ ions in the bulk of polymer films, containing PLA, silver palmitate as a precursor of Ag NP and reducing agent (PEI or chitosan), has been performed by keeping them at 100–170 °C within 5 minutes. The polymeric film composites, cast from chloroform solution, were heated in an oven using precise thermal regulator VRT-3. As a result of reduction, the films get attained silver color, the Ag content in the bulk of films varied from 1 to 4 wt. %, and the thickness of the films was 110 μm. Structure, morphology, thermomechanical and antimicrobial properties of two types of nanocomposites – PLA-Ag-PEI and PLA-Ag-chitosan, formed by the thermochemical reduction of Ag+ in polymer films have been studied using wide-angle X-ray scattering (WAXS) (a DRON-4-07 diffractometer), a transmission electron microscope (TEM) (JEM-1230 JEOL, Japan), and thermomechanical analysis (a UIP-70M device). Antimicrobial activity of the obtained nanocomposites was investigated applying reference strains of opportunistic bacteria Staphylococcus aureus and Escherichia coli. It has been found that thermochemical reduction of Ag+ ions in the bulk of polymer films, when using synthetic or natural polymers (PEI or chitosan) as a reducing and stabilizing agent of silver nanoparticles occurs at 160 °C during 5 minutes. It has been found that the average size of Ag nanoparticles in the polymeric matrix is equal to ~ 7 and ~ 4 nm for PEI and chitosan, respectively. It has been shown that PLA-Ag-chitosan nanocomposites have much higher antimicrobial activity against S. aureus and E. coli strains as compared to PLA-Ag-PEI nanocomposites.