bluuebunny/crossref_metadata_2025 huggingface dataset:
Management of take-all disease caused by Gaeumannomyces graminis var. tritici in wheat through Bacillus subtilis strains
Authors: Zhao Gangyi , Sun Tianjie , Zhang Zina , Zhang Jingjing , Bian Yinbo , Hou Chunyan , Zhang Dongdong , Han Shengfang , Wang Dongmei
Published: February 2023

Wheat (Triticum aestivum) is the second largest grain crop worldwide, and one of the three major grain crops produced in China. Take-all disease, caused by Gaeumannomyces graminis var. tritici (Ggt) infection, is a widespread and devastating soil-borne disease that harms wheat production. At present, the prevention and control of wheat take-all depend largely on the application of chemical pesticides. Chemical pesticides, however, not only lead to increased drug resistance of pathogens but also leave significant residues in the soil, causing serious environmental pollution. In this study, we investigated the application of Bacillus subtilis to achieve take-all disease control in wheat while reducing pesticide application. Antagonistic bacteria were screened by plate test, species identification of strains was performed by Gram staining and sequencing of 16s rDNA, secondary metabolite activity of strains was detected by clear circle method, strain compatibility and effect of compounding on Ggt were detected by plate, and the application prospects of specific strains were analyzed by greenhouse and field experiments. We found that five B. subtilis strains, JY122, JY214, ZY133, NW03, Z-14, had significant antagonistic effects against Ggt, and could secrete antimicrobial proteins including amylase, protease, and cellulase. Furthermore, Z-14 and JY214 cultures have also been shown to change the morphology of Ggt mycelium. These results also showed that Z-14, JY214, and their combination can control take-all disease in wheat at a reduced level of pesticide use. In summary, we screened two Bacillus spp. strains, Z-14 and JY214, that could act as antagonists that contribute to the biological control of wheat take-all disease. These findings provide resources and ideas for controlling crop diseases in an environmentally friendly manner.
Effects of inhaled cannabis high in Δ9-THC or CBD on the aging brain: A translational MRI and behavioral study
Authors: Sadaka Aymen H. , Canuel Justin , Febo Marcelo , Johnson Clare T. , Bradshaw Heather B. , Ortiz Richard , Ciumo Federica , Kulkarni Praveen , Gitcho Michael A. , Ferris Craig F.
Published: February 2023

With the recent legalization of inhaled cannabis for medicinal and recreational use, the elderly represents one of the newest, rapidly growing cohorts of cannabis users. To understand the neurobiological effects of cannabis on the aging brain, 19–20 months old mice were divided into three groups exposed to vaporized cannabis containing ~10% Δ9-THC, ~10% CBD, or placebo for 30 min each day. Voxel based morphometry, diffusion weighted imaging, and resting state functional connectivity data were gathered after 28 days of exposure and following a two-week washout period. Tail-flick, open field, and novel object preference tests were conducted to explore analgesic, anxiolytic, and cognitive effects of cannabis, respectively. Vaporized cannabis high in Δ9-THC and CBD achieved blood levels reported in human users. Mice showed antinociceptive effects to chronic Δ9-THC without tolerance while the anxiolytic and cognitive effects of Δ9-THC waned with treatment. CBD had no effect on any of the behavioral measures. Voxel based morphometry showed a decrease in midbrain dopaminergic volume to chronic Δ9-THC followed but an increase after a two-week washout. Fractional anisotropy values were reduced in the same area by chronic Δ9-THC, suggesting a reduction in gray matter volume. Cannabis high in CBD but not THC increased network strength and efficiency, an effect that persisted after washout. These data would indicate chronic use of inhaled cannabis high in Δ9-THC can be an effective analgesic but not for treatment of anxiety or cognitive decline. The dopaminergic midbrain system was sensitive to chronic Δ9-THC but not CBD showing robust plasticity in volume and water diffusivity prior to and following drug cessation an effect possibly related to the abuse liability of Δ9-THC. Chronic inhaled CBD resulted in enhanced global network connectivity that persisted after drug cessation. The behavioral consequences of this sustained change in brain connectivity remain to be determined.
The relationship between the interictal epileptiform discharge source connectivity and cortical structural couplings in temporal lobe epilepsy
Authors: Li Zhensheng , Jiang Che , Gao Quwen , Xiang Wei , Qi Zijuan , Peng Kairun , Lin Jian , Wang Wei , Deng Bingmei , Wang Weimin
Published: February 2023

Objective

The objective of this study was to explore the relation between interictal epileptiform discharge (IED) source connectivity and cortical structural couplings (SCs) in temporal lobe epilepsy (TLE).

Methods

High-resolution 3D-MRI and 32-sensor EEG data from 59 patients with TLE were collected. Principal component analysis was performed on the morphological data on MRI to obtain the cortical SCs. IEDs were labeled from EEG data and averaged. The standard low-resolution electromagnetic tomography analysis was performed to locate the source of the average IEDs. Phase-locked value was used to evaluate the IED source connectivity. Finally, correlation analysis was used to compare the IED source connectivity and the cortical SCs.

Results

The features of the cortical morphology in left and right TLE were similar across four cortical SCs, which could be mainly described as the default mode network, limbic regions, connections bilateral medial temporal, and connections through the ipsilateral insula. The IED source connectivity at the regions of interest was negatively correlated with the corresponding cortical SCs.

Significance

The cortical SCs were confirmed to be negatively related to IED source connectivity in patients with TLE as detected with MRI and EEG coregistered data. These findings suggest the important role of intervening IEDs in treating TLE.
Involvement of the GABAergic system in PTSD and its therapeutic significance
Authors: Huang Junhui , Xu Fei , Yang Liping , Tuolihong Lina , Wang Xiaoyu , Du Zibo , Zhang Yiqi , Yin Xuanlin , Li Yingjun , Lu Kangrong , Wang Wanshan
Published: February 2023

The neurobiological mechanism of post-traumatic stress disorder (PTSD) is poorly understood. The inhibition of GABA neurons, especially in the amygdala, is crucial for the precise regulation of the consolidation, expression, and extinction of fear conditioning. The GABAergic system is involved in the pathophysiological process of PTSD, with several studies demonstrating that the function of the GABAergic system decreases in PTSD patients. This paper reviews the preclinical and clinical studies, neuroimaging techniques, and pharmacological studies of the GABAergic system in PTSD and summarizes the role of the GABAergic system in PTSD. Understanding the role of the GABAergic system in PTSD and searching for new drug targets will be helpful in the treatment of PTSD.
Characterization of the structural, physicochemical, and functional properties of soluble dietary fibers obtained from the peanut shell using different extraction methods
Authors: Wang Lei , Fan Rui , Yan Yanhua , Yang Shuo , Wang Xuesong , Zheng Baiqin
Published: February 2023

Objective

To propose a possible solution for a peanut by-product, peanut shell (PS), this study evaluated the effects of different methods, including enzymatic extraction (E-SDF), microwave extraction (M-SDF), and pulsed electric field extraction (PEF-SDF), on the characterization of soluble dietary fibers (SDFs) from PS.

Methods

We determined the physicochemical properties, including water- and oil-holding capacities (WHC and OHC), emulsifying properties, rheological properties, functional properties, including pancreatic lipase activity inhibition (PRAI), glucose and cholesterol adsorption capacities (GAC and CAC), and the structural properties of SDFs.

Results

The results showed that PEF-SDF possessed the highest WHC, OHC, and emulsifying properties. M-SDF and PEF-SDF appeared to have more complex and porous structures, and they showed small molecular weights. Notably, PEF-SDF showed the strongest capacities in CAC, GAC, and PRAI.

Conclusions

The results indicate that PEF-SDF is a potential SDF preparation method for a promising dietary fiber (DF) source, PS.
Impact of nutritional diet therapy on premenstrual syndrome
Authors: Siminiuc Rodica , Ţurcanu Dinu
Published: February 2023

Premenstrual syndrome (PMS) is one of the most common disorders faced by women of reproductive age. More than 200 symptoms of varying severity associated with PMS have been identified. Because of the broad spectrum of action of PMS and its impact on quality of life, symptom relief is the main challenge of treating PMS and premenstrual dysphoric disorder (PMDD). The review aims to analyze and identify the potential impact of dietary and nutritional therapies on PMS and, respectively, for its better management. The study was conducted by accessing Internet databases such as PubMed, ScienceDirect, and Scopus and using relevant keywords such as PMS, symptoms, dietary patterns (DPs), macro and micronutrients, and supplements. The results showed that diet is an essential modulating factor in reducing and managing PMS symptoms. But research on the actual effect of foods and nutrients on PMS is sparse, sporadic, and studied with insufficient scientific rigor. No correlations were identified between the consumption of macronutrients and PMS: protein, fat, carbohydrates, and fiber, but the effectiveness of micronutrients, especially calcium, magnesium, vitamin D, B vitamins, and herbal supplements, was demonstrated. Researchers remain unanimous that the evidence is insufficient and limited to support their use as an effective treatment. Nevertheless, the results could contribute to providing quality information to help women and girls make evidence-based decisions regarding premenstrual health and the adoption of dietary and nutritional therapies.
Construction of nano slow-release systems for antibacterial active substances and its applications: A comprehensive review
Authors: Cao Jiayong , Gao Mingkun , Wang Jian , Liu Yuan , Zhang Xuan , Ping Yi , Liu Jia , Chen Ge , Xu Donghui , Huang Xiaodong , Liu Guangyang
Published: February 2023

At present, nano-carrier materials with antibacterial activity are of great significance. Due to the widespread resistance of many pathogenic microorganisms, it has seriously threatened human health. The natural antimicrobial substances extracted from fruits and vegetables can significantly improve their stability combined with nano-carrier materials. The resistance of pathogenic microorganisms will be substantially reduced, greatly enhancing the effect of active antimicrobial substances. Nanotechnology has excellent research prospects in the food industry, antibacterial preservation, food additives, food packaging, and other fields. This paper introduces nano-carrier materials and preparation techniques for loading and encapsulating active antibacterial substances in detail by constructing a nano-release system for active antibacterial substances. The antibacterial effect can be achieved by protecting them from adverse external conditions and destroying the membrane of pathogenic microorganisms. The mechanism of the slow release of the bacteriostatic active substance is also described. The mechanism of carrier loading and release is mainly through non-covalent forces between the bacteriostatic active substance and the carrier material, such as hydrogen bonding, π-π stacking, van der Waals forces, electrostatic interactions, etc., as well as the loading and adsorption of the bacteriostatic active substance by the chemical assembly. Finally, its wide application in food and medicine is introduced. It is hoped to provide a theoretical basis and technical support for the efficient utilization and product development of bacteriostatic active substances.
Combination of ultrafast dynamic contrast-enhanced MRI-based radiomics and artificial neural network in assessing BI-RADS 4 breast lesions: Potential to avoid unnecessary biopsies
Authors: Lyu Yidong , Chen Yan , Meng Lingsong , Guo Jinxia , Zhan Xiangyu , Chen Zhuo , Yan Wenjun , Zhang Yuyan , Zhao Xin , Zhang Yanwu
Published: February 2023

Objectives

To investigate whether combining radiomics extracted from ultrafast dynamic contrast-enhanced MRI (DCE-MRI) with an artificial neural network enables differentiation of MR BI-RADS 4 breast lesions and thereby avoids false-positive biopsies.

Methods

This retrospective study consecutively included patients with MR BI-RADS 4 lesions. The ultrafast imaging was performed using Differential sub-sampling with cartesian ordering (DISCO) technique and the tenth and fifteenth postcontrast DISCO images (DISCO-10 and DISCO-15) were selected for further analysis. An experienced radiologist used freely available software (FAE) to perform radiomics extraction. After principal component analysis (PCA), a multilayer perceptron artificial neural network (ANN) to distinguish between malignant and benign lesions was developed and tested using a random allocation approach. ROC analysis was performed to evaluate the diagnostic performance.

Results

173 patients (mean age 43.1 years, range 18–69 years) with 182 lesions (95 benign, 87 malignant) were included. Three types of independent principal components were obtained from the radiomics based on DISCO-10, DISCO-15, and their combination, respectively. In the testing dataset, ANN models showed excellent diagnostic performance with AUC values of 0.915-0.956. Applying the high-sensitivity cutoffs identified in the training dataset demonstrated the potential to reduce the number of unnecessary biopsies by 63.33%-83.33% at the price of one false-negative diagnosis within the testing dataset.

Conclusions

The ultrafast DCE-MRI radiomics-based machine learning model could classify MR BI-RADS category 4 lesions into benign or malignant, highlighting its potential for future application as a new tool for clinical diagnosis.
Spatially resolved transcriptomics revealed local invasion-related genes in colorectal cancer
Authors: Liu Hong-Tao , Chen Si-Yuan , Peng Ling-Long , Zhong Li , Zhou Li , Liao Si-Qi , Chen Zhi-Ji , Wang Qing-Liang , He Song , Zhou Zhi-Hang
Published: February 2023

Objective

Local invasion is the first step of metastasis, the main cause of colorectal cancer (CRC)-related death. Recent studies have revealed extensive intertumoral and intratumoral heterogeneity. Here, we focused on revealing local invasion-related genes in CRC.

Methods

We used spatial transcriptomic techniques to study the process of local invasion in four CRC tissues. First, we compared the pre-cancerous, cancer center, and invasive margin in one section (S115) and used pseudo-time analysis to reveal the differentiation trajectories from cancer center to invasive margin. Next, we performed immunohistochemical staining for RPL5, STC1, AKR1B1, CD47, and HLA-A on CRC samples. Moreover, we knocked down AKR1B1 in CRC cell lines and performed CCK-8, wound healing, and transwell assays to assess cell proliferation, migration, and invasion.

Results

We demonstrated that 13 genes were overexpressed in invasive clusters, among which the expression of CSTB and TM4SF1 was correlated with poor PFS in CRC patients. The ribosome pathway was increased, while the antigen processing and presentation pathway was decreased along CRC progression. RPL5 was upregulated, while HLA-A was downregulated along cancer invasion in CRC samples. Pseudo-time analysis revealed that STC1, AKR1B1, SIRPA, C4orf3, EDNRA, CES1, PRRX1, EMP1, PPIB, PLTP, SULF2, and EGFL6 were unpregulated along the trajectories. Immunohistochemic3al staining showed the expression of STC1, AKR1B1, and CD47 was increased along cancer invasion in CRC samples. Knockdown of AKR1B1 inhibited CRC cells’ proliferation, migration, and invasion.

Conclusions

We revealed the spatial heterogeneity within CRC tissues and uncovered some novel genes that were associated with CRC invasion.
Unexpected breast cancer mimicking benign lesions on ultrasound-guided vacuum-assisted excision biopsy: A retrospective cross-sectional study over a 20-year period
Authors: Zhou Wu , Luo Honghao , Zhao Haina , Peng Yulan
Published: February 2023

Objectives

This study investigated the occurrence rate of unexpected breast cancer (UEBC) mimicking benign lesions [Breast Imaging Reporting and Data System (BI-RADS) category 3 or 4a] using ultrasound-guided vacuum-assisted excision biopsy (US-VAEB), and explored the factors responsible for late diagnosis of T2 stage UEBC.

Materials and methods

We collected clinicopathologic data and preoperative US imaging features within 3 months before US-VAEB of patients who were diagnosed with UEBC from January 2002 to September 2022. The UEBC were divided into T1 and T2 stageUEBC. The US imaging features as well as clinical and pathological information of T1 and T2 stage UEBC were compared to explore the factors responsible for late diagnosis of T2 stage UEBC.

Results

Breast cancer was diagnosed in 91 of 19 306 patients who underwent US-VAEB. We excluded eight patients with breast cancer assigned to BI-RADS 4b category by preoperative US, and two for whom US imaging records were unavailable. Finally, we enrolled 81 patients. The occurrence rate of UEBC after US-VAEB was 0.42%(81/19296). Of the 81 cases of UEBC, 22 were at T2 stage. The ratio of T2 stage UEBC was 27.2%. The differences in risk factor of breast cancer and routine breast US screening between T1 and T2 stage UEBC were significant[96.6% (57/59) vs 81.8% (18/22), 44.1% (26/59) vs 13.6% (3/22), respectively, P<0.05).

Conclusion

UEBC was rarely detected by US-VAEB. Most cases of T2 stage UEBC were diagnosed late because of the absence of routine US screening and risk factors for breast cancer. Stricter clinical management regulations for breast lesions and performing regular US screening may be helpful to reduce T2 stage UEBC.
The surge of RSV and other respiratory viruses among children during the second COVID-19 pandemic winter season
Authors: Riepl Angela , Straßmayr Lena , Voitl Peter , Ehlmaier Paulina , Voitl Julian J. M. , Langer Klara , Kuzio Ulrike , Mühl-Riegler Alexandra , Mühl Bernhard , Diesner-Treiber Susanne C.
Published: February 2023

Background

The non-pharmaceutical measures in the first Covid-19 winter season significantly impacted respiratory pathogens such as RSV, influenza, or metapneumovirus, which cause respiratory infections, especially in infants and young children. This longitudinal prospective study aimed to determine how less strict measures affect the pathogen profile in the second winter season.

Methods

From September 2021 till the end of March 2022, 678 children (0–36 months) admitted to Vienna's largest pediatric center with an acute respiratory infection were enrolled in this study. The researchers performed nasal swabs and tested them by multiplex PCR for 23 respiratory pathogens, chronicled clinical features and treatment, and analyzed the effect of lockdown on the pathogen prevalence.

Results

The 815 smears of 678 children revealed the most common pathogens to be rhino-/enterovirus (38.5%), RSV (26.7%), and metapneumovirus (7.2%). The lockdown interrupted the early RSV onset in September [RR 0.367, CI (0.184–0.767), p = 0.003], while no effects on the other pathogens were found. Metapneumovirus started circulating in January. Influenza was only sporadically detected. The hospitalization rate was significantly higher than last season due to RSV [OR 4.089, 95%CI (1.414–11.827), p-adj = 0.05].

Conclusion

With more flexible non-pharmaceutical measures, children aged 0–36 months started presenting again with viral pathogens, such as RSV and metapneumovirus. RSV, associated with a high hospitalization rate, had a very early onset with an abrupt interruption due to the only lockdown.
Linear plasma device GyM for plasma-material interaction studies
Authors: Uccello Andrea , Bin William , Bruschi Alessandro , Causa Federica , Cremona Anna , De Angeli Marco , Farina Daniela , Gatto Giuseppe , Gervasini Gabriele , Ghezzi Francesco , Gittini Giuseppe , Granucci Gustavo , Grosso Giovanni , Laguardia Laura , Lontano Maurizio , Mellera Vittoria , Minelli Daniele , Nardone Antonio , Pedroni Matteo , Ripamonti Federico , Rispoli Natale , Vassallo Espedito , Ricci Daria
Published: February 2023

GyM is a linear plasma device operating at Istituto per la Scienza e Tecnologia dei Plasmi, Consiglio Nazionale delle Ricerche, Milan, with the original aim of studying basic plasma physics, such as turbulent processes. Since 2014, GyM experimental program has been mainly focused on the issue of plasma-material interaction (PMI) for magnetic confinement nuclear fusion applications. GyM consists of a stainless steel vacuum chamber (radius and length of 0.125 m and 2.11 m), a pumping system, a gas injection system, 10 magnetic field coils and two magnetron sources at 2.45 GHz, capable of delivering a total microwave power up to 4.5 kW. Highly reproducible steady-state plasmas of different gas species, at a maximum working pressure of ∼10−1 Pa, can be obtained by electron cyclotron resonance heating in the resonance layer at 87.5 mT. Plasmas of GyM have electron and ion temperature ≤15 eV and ∼0.1 eV, respectively. The electron density is in the range of 1015–1017 m−3 and the ion flux is ≤5 × 1020 ions⋅m−2s−1. Main plasma diagnostics of GyM comprise Langmuir probes, an optical emission spectrometer, a mass spectrometer and a fast camera system equipped with an image intensifier unit. For the purpose of investigating the topic of PMI, GyM is provided with two sample exposure systems. Both are biasable at a negative bias voltage down to −400 V, to tune the energy of the impinging ions. One of them is also equipped with a heating lamp and can reach and sustain a temperature of 990 K for several hours, thus allowing to study the role of sample temperature during the plasma-material interaction. This contribution presents the layout of GyM, the diagnostics, the sample exposure systems and the typical plasma parameters. A brief overview of the main PMI activities carried out so far and a description of future machine upgrades are also given.
Defect-induced magnetism in TiO2: An example of quasi 2D magnetic order with perpendicular anisotropy
Authors: Stiller Markus , Esquinazi Pablo D.
Published: February 2023

Magnetic order at room temperature induced by atomic lattice defects, like vacancies, interstitials, or their pairs, has been observed in a large number of different non-magnetic hosts, such as pure graphite, oxides, and silicon-based materials. High Curie temperatures and time-independent magnetic response at room temperature indicate the extraordinary robustness of this new phenomenon in solid-state magnetism. In this work, we review experimental and theoretical results of pure TiO2 (anatase), whose magnetic order can be triggered by low-energy ion irradiation. In particular, we discuss the systematic observation of an ultrathin magnetic layer with perpendicular magnetic anisotropy at the surface of this oxide.
Older adults’ refusal speech act in cognitive assessment: A multimodal pragmatic perspective
Authors: Huang Lihe , Qu Huiyu , Zhou Deyu
Published: February 2023

This paper explores how older adults with different cognitive abilities perform the refusal speech act in the cognitive assessment in the setting of memory clinics. The refusal speech act and its corresponding illocutionary force produced by nine Chinese older adults in the Montreal Cognitive Assessment-Basic was annotated and analyzed from a multimodal perspective. Overall, regardless of the older adults’ cognitive ability, the most common discursive device to refuse is the demonstration of their inability to carry out or continue the cognitive task. Individuals with lower cognitive ability were found to perform the refusal illocutionary force (hereafter RIF) with higher frequency and degree. Additionally, under the pragmatic compensation mechanism, which is influenced by cognitive ability, multiple expression devices (including prosodic features and non-verbal acts) interact dynamically and synergistically to help older adults carry out the refusal behavior and to unfold older adults’ intentional state and emotion as well. The findings indicate that both the degree and the frequency of performing the refusal speech act in the cognitive assessment are related to the cognitive ability of older adults.
Letting go or giving up? The influence of self-transcendence meaning of life on goal adjustment in high action crisis
Authors: Hu Xinyi , Zhang Heyi , Geng Meifang
Published: February 2023

When individuals pursuing personal goals encounter setbacks and failures, they often fall into a conflict between disengaging from and striving toward the goal, defined as an action crisis. The present study investigated the influence and mechanism of self-transcendence meaning of life (STML) on goal disengagement and reengagement during a high versus a low action crisis. Study 1 included situations with different action crises. In Study 1, participants with high STML exhibited significantly higher goal disengagement and reengagement during high action crisis compared with low action crisis. Study 2 replicated the findings in Study 1 using participants’ personal goals by questionnaires, and further exhibited that action crisis had negative effect on self-efficacy for participants with low STML, and this process subsequently reduced goal adjustment. Interestingly, no mediation effect of self-efficacy was found between action crisis and goal adjustment among participants with high STML. Findings from the present study suggest that releasing obsessions and adopting a dialectical relationship between success and failure may help individuals in high action-crisis situations, and self-efficacy may provide flexibility and autonomy.
Live stream marketing and consumers’ purchase intention: An IT affordance perspective using the S-O-R paradigm
Authors: Zhang Lu , Chen Min , Zamil Ahmad M. A.
Published: February 2023

Recent years have seen a shift in the online retail industry toward a greater emphasis on live marketing. The growth of social media commerce on the internet demonstrates the popularity of Livestream shopping. Although there has been a rise in interest in studying live streaming, a comprehensive model describing why consumers are willing to consistently employ this novel sales format has yet to be developed. Hence, the present study develops a model to determine the factors influencing consumers’ live-stream shopping intention by employing the affordance lens and S-O-R model. The online data was collected through the Wenjuanxing website from the users of live-streaming platforms such as Taobao.com, Mogujie.com, and JD.com. Results showed that (N = 434): trust can be enhanced through visibility, metavoicing, trading affordance, guidance shopping and interactivity that consequently affects consumer purchase intention. In addition, this study highlights the theoretical and managerial implications for social commerce.
Intelligent ground vibration prediction in surface mines using an efficient soft computing method based on field data
Authors: Keshtegar Behrooz , Piri Jamshid , Asnida Abdullah Rini , Hasanipanah Mahdi , Muayad Sabri Sabri Mohanad , Nguyen Le Binh
Published: February 2023

Ground vibration induced by blasting operations is considered one of the most common environmental effects of mining projects. A strong ground vibration can destroy buildings and structures, hence its prediction and minimization are of high importance. The aim of this study is to estimate the ground vibration through a hybrid soft computing (SC) method, called RSM-SVR, which comprises two main regression techniques: the response surface model (RSM) and support vector regression (SVR). The RSM-SVR model applies an RSM in the first calibrating process and an SVR in the second calibrating process to improve the accuracy of the ground vibration predictions. The predicted results of an RSM, which are obtained using the input data of problems, are used as the input dataset for the regression process of an SVR. The effectiveness and agreement of the RSM-SVR model were compared to those of an SVR optimized with the particle swarm optimization (PSO) and genetic algorithm (GA), RSM, and multivariate linear regression (MLR) based on several statistical factors. The findings confirmed that the RSM-SVR model was considerably superior to other models in terms of accuracy. The amounts of coefficient of determination (R2) were 0.896, 0.807, 0.782, 0.752, 0.711, and 0.664 obtained from the RSM-SVR, PSO-SVR, GA-SVR, MLR, SVR, and RSM models, respectively.
An overview of Iran's actions in response to the COVID-19 pandemic and in building health system resilience
Authors: Gouya Mohammad-Mehdi , Seif-Farahi Katayoun , Hemmati Payman
Published: February 2023

This article is part of the Research Topic ‘Health Systems Recovery in the Context of COVID-19 and Protracted Conflict'. The considerable human, social, and economic impacts of COVID-19 have demonstrated a global lack of health system resilience, highlighting gaps in health system capacities due to fragmented approaches to health system financing, planning, and implementation. One of the key actions for ensuring equitable essential health services in all countries in normal situations as well as emergencies is through strengthening the primary healthcare (PHC) system. In the context of the unfolding pandemic, the Iranian Ministry of Health and Medical Education (MoHME) undertook a variety of strategic actions to ensure the sustainability of health services during the current health emergency and to promote health system resilience against future shocks. Right after the Alma-Ata declaration in 1978, MoHME pursued the PHC philosophy incorporating the principles within the WHO health system framework and its six building blocks. In response to the evolving pandemic, MoHME put in place several interventions to ensure the maintenance of essential health services in addition to the provision of response. Some interventions were new, informed by global experience with COVID-19, while others leveraged existing strengths within the existing health system. Those were taking a whole-of-government approach; leveraging the PHC capacity; supporting the workforce; strengthening preparedness and response; improving access to medicines, vaccines, and health products; and leveraging the health information system into the pandemic response. Health system strengthening that promotes resilience is imperative for governments as health systems are fundamental to sustainable socioeconomic development. In recognition of this, the WHO Eastern Mediterranean Regional Office (EMRO) has recently outlined regional priorities for advancing universal health coverage (UHC) and ensuring health security. Iran's approach both prior to and during the pandemic is strongly aligned with those regional priorities, which are “primary health care-oriented models; enhancing health workforce; promoting equity; enabling environment for research; improving access to countermeasures; and fostering health system resilience.”
Impact of COVID-19 pandemic on relapse of individuals with severe mental illness and their caregiver's burden
Authors: Nooraeen Sara , Bazargan-Hejazi Shahrzad , Naserbakht Morteza , Vahidi Camelia , Shojaerad Farideh , Mousavi Seyedeh Sahar , Malakouti Seyed Kazem
Published: February 2023

Background

The implementation of quarantine and social distancing measures to control the COVID-19 pandemic led to restrictions at the community level and most of in-person psychiatric services were discontinued. This situation could affect the psychopathology of the patients and the burden of their caregivers. The aim of this study was to investigate the effects of COVID-19 pandemic on people with severe mental illnesses (SMIs) and their caregivers' burden.

Method

The study sample consisted of 86 patients with severe mental illness and 86 caregivers. The mental status, relapse rate, and rehospitalization rate of the patients and the general health status and burden of caregivers were investigated in three waves, including before and 3 and 6 months after the COVID-19 pandemic.

Results

The relapse rate of the patients was 14%, 33.7%, and 43% (p = 0.000) and the rehospitalization rate was 4.7%, 7%, and 10.5% in waves 0, 1, and 2, respectively (p = 0.000). Most of the psychopathological scales increased in three waves (p = 0.000). The caregivers' burden and health condition worsened during the nine months of the study as well (p = 0.000).

Conclusion

The COVID-19 pandemic led to the exacerbation of symptoms and increased the relapse rate in people with SMIs. It also worsened the caregivers' condition. People with severe mental illnesses (SMIs) and their caregivers are one of the most vulnerable groups on which the COVID-19 pandemic had a marked negative effect.
Comparison of copper concentration between non-alcoholic fatty liver disease patients and normal individuals: A meta-analysis
Authors: Chen Yanfang , Wu Chutian , Li Guanhong , Wang Wen , Tang Shaohui
Published: February 2023

Non-alcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver disease worldwide. Copper metabolism plays an important role in the pathogenesis of NAFLD. However, the relationship between serum/hepatic copper concentration and NAFLD is still debated. A literature search was performed using electronic databases to find publications up to September 2022, where the relationship between serum/hepatic copper or ceruloplasmin concentration and NAFLD was evaluated. Finally, 6 articles with 9 unique outcomes involving 2,607 NAFLD patients and 1,441 non-NAFLD normal individuals were included. The pooled results showed that hepatic copper concentration was significantly decreased in NAFLD patients (SMD = −0.98, 95% CI = [−1.21; −0.74], p < 0.0001), and the sensitivity analysis also confirmed this. Nevertheless, serum copper (SMD = −0.02, 95% CI = [−0.32; 0.28], p = 0.88) and ceruloplasmin (SMD = −0.03, 95% CI = [−0.69; 0.63], p = 0.93) were not associated with NAFLD. This meta-analysis revealed that low hepatic copper concentration was found in NAFLD patients and serum copper and ceruloplasmin were not associated with NAFLD. Larger cohort studies and related trials are needed to further validate the result of this meta-analysis in the future.
Inclusive community playgrounds benefit typically developing children: An objective analysis of physical activity
Authors: Stevens Wilshaw R. , Borchard Justine M. , Sleeper Paige , Dempsey Dana , Jeans Kelly A. , Jo Chan-Hee , Tulchin-Francis Kirsten
Published: February 2023

Purpose

Limited research is available on the physical activity levels of children while playing on an inclusive playground, specifically designed to accommodate children with physical disabilities. The aims of this study were to objectively measure ambulatory activity and heart rate (HR) of children during unstructured play on an inclusive community playground.

Methods

Typically developing children at least 4 years of age were recruited to play freely upon entering the playground. Participants wore a StepWatch4 Activity Monitor and a Polar V800 Sport Watch. Ambulatory measures included total steps, percentage of recommended steps, total ambulatory time (TAT), bout intensity levels/duration periods. Time spent in HR zones and moderate-to-vigorous physical activity (MVPA) was determined.

Results

95 children (48 males; Avg. age: 7 ± 2 years.) were included in this study. Children played for 31.8 ± 14.7 min., were ambulatory for 25.9 ± 12.0 min., took 1826 ± 824 steps, and accumulated 17 ± 8% of the recommended daily step count. Ambulatory bout intensity was predominantly lower intensity and bout durations varied in length. 99% of the play time was spent at a moderate HR or higher. Significant correlations were found between ambulatory and HR measures (ρ range from 0.23 to 0.99, p < 0.05), and 7–10 yo children spent a significantly higher percentage of TAT at higher intensity ambulation (p < 0.05).

Conclusions

Typically developing children can achieve moderate or higher intensity exercise and HR on an inclusive playground. Both typically developing children and those with disabilities, would benefit from a setting where they can interact and participate in parallel play with their peers.
Disappearance and survival of fermented beverages in the biosphere reserve Tehuacán-Cuicatlán, Mexico: The cases of Tolonche and Lapo
Authors: Ojeda-Linares César Iván , Vallejo Mariana , Casas Alejandro
Published: February 2023

Traditional fermented products are disappearing from the local foodscapes due to social pressures and ecological changes that affect their production; it is therefore crucial to document local knowledge, which is crucial to maintain and recover local biocultural heritage and to contribute to food security. This study aims to document and foster the production of local traditional beverages by registering recipes of fermented beverages in the Tehuacán-Cuicatlán biosphere reserve in central Mexico, a region recognized for its great biocultural diversity. We conducted a search of peer-reviewed literature. Additionally, we included ethnographic research and participatory methods to engage residents in different steps of the production process. We identified five main fermented beverages in the research area, the most common beverages are those produced by agave species which include, mescal, pulque and an almost extinct beverage known as lapo which involves sugar cane as main substrate. We also identified a fermented beverage produced with several cacti fruits known as nochoctli and a traditional a fermented beverage produced with fruits of Schinus molle known as tolonche. We highlight the production of lapo and tolonche since these involved the incorporation of foreign substrates into the region after the Spaniard conquest and to their restricted distribution and almost extinction. The beverages tolonche and lapo are nowadays almost lost and only a few producers still prepare them to follow modified versions of the original recipe. Lapo and tolonche were once important in the research area but almost became extinct until local people started to recently recover them. Traditional fermented beverages in Mexico play an important role in cultural identity and contribute to the local diet; nevertheless, several fermented beverages have not been recorded and have even become extinct. This work is an effort to promote and conserve traditional fermented beverages as valuable biocultural heritage by empowering people to make decisions about the use of locally available resources, which is crucial in times when food systems are highly vulnerable.
Study of the effectiveness of glucosamine and chondroitin sulfate, marine based fatty acid compounds (PCSO-524 and EAB-277), and carprofen for the treatment of dogs with hip osteoarthritis: A prospective, block-randomized, double-blinded, placebo-controlled clinical trial
Authors: Kampa Naruepon , Kaenkangploo Duangdaun , Jitpean Supranee , Srithunyarat Thanikul , Seesupa Suvaluk , Hoisang Somphong , Yongvanit Karn , Kamlangchai Phanthit , Tuchpramuk Pongsatorn , Lascelles B. Duncan X.
Published: February 2023

Introduction

Glucosamine hydrochloride and chondroitin sulfate are commonly used in dogs with OA, but evidence around efficacy is mixed. This study evaluated the effectiveness of glucosamine and chondroitin sulfate, marine based fatty acid compounds (PCSO-524 and EAB-277), and carprofen for the alleviation of canine hip OA pain. This was a prospective, block-randomized, double-blinded, placebo-controlled clinical trial.

Methods

Seventy-five owned pet dogs with hip OA were assigned randomly into five treatment groups: PCSO-524, Glucosamine and chondroitin sulfate, EAB-277, carprofen, and Placebo (sunflower oil). Peak vertical force (PVF) and subjective orthopedic assessment scores (OAS) were evaluated before treatment (week 0), and at weeks 2, 4, and 6 during treatment.

Results

At week 2, the carprofen group showed a significant increase in PVF (3.14 ± 5.33; mean ± SD). After 4 weeks, the increases in PVF of the PCSO-524 (3.90 ± 3.52), EAB-277 (4.17 ± 4.94), and carprofen (3.08 ± 5.87) groups were significant, and significantly greater than placebo (0.08 ± 1.90) and glucosamine (−0.05 ± 6.34) groups. After 6 weeks, the change of PVF in the PCSO-524 (4.14 ± 4.65), EAB-277 (4.45 ± 4.23), and carprofen (4.21 ± 6.52) groups were significant and significantly higher than the placebo group (−0.33 ± 3.65). The change in PVF in the glucosamine group (1.08 ± 5.49) lay between the placebo group and the other treatment groups. The OAS did not show any significant change in any group.

Discussion

PCSO-524 and EAB-277, but not glucosamine/chondroitin, resulted in significant improvements in PVF from baseline after 4 weeks, and 6 weeks, and to a similar degree to that seen with carprofen.
Non-Intersecting Diverging Runways Separation under Emergency Avoidance Situation
Authors: Li Changcheng , Hu Minghua , Zhao Zheng , Hu Yuxin , Peng Ying
Published: February 2023

Although runway separation, based on the probability of collision, has been studied for decades, the mathematical methods proposed by the majority of studies cannot handle complex situations, such as the operation of non-intersecting diverging runways at an airport with multiple runways. By applying a combination method of computer simulation and collision probability calculation, the arrival and departure window (ADW) separation for non-intersecting diverging runways of a multi-runway airport was studied under the emergency avoidance (EA) situation. Combining the example of runways 01L/19R and 11L of Beijing Daxing Airport, the ADW separation settings for the airport’s northward and southward operations were determined to meet the target level of safety. Moreover, the effects of range-type parameters on the ADW separation were quantified. When the EA maximum speed limit and EA minimum climb rate were 200 kt (102.9 m/s) and 10%, respectively, the results were such that no ADW separation was required for northward operation, and the ADW separation was from 3.2 km to 7.1 km for southward operation. Furthermore, the results showed that the proposed method could more accurately describe the nominal trajectories of aircraft and improve the precision of collision probability calculation. Meanwhile, the sensitivity analysis method for range-type parameters could help airports and air traffic control facilities to set reasonable constraints to improve theoretical runway capacity, while satisfying practical feasibility.
Antibiotic Prescription Patterns for Acute Respiratory Infections in Rural Primary Healthcare Settings in Guangdong, China: Analysis of 162,742 Outpatient Prescriptions
Authors: Wang Jiong , Li Feifeng , Chen Zhixu , Guo Yingyi , Liu Ningjing , Liu Baomo , Xiao Shunian , Yao Likang , Li Jiahui , Zhuo Chuyue , He Nanhao , Zou Guanyang , Zhuo Chao
Published: February 2023

Overuse and inappropriate use of antibiotics are important contributors to bacterial antimicrobial resistance (AMR), especially in ambulatory primary healthcare (PHC) settings in low- and middle-income countries. This study aimed to investigate antibiotic prescription patterns among patients with acute respiratory infections (ARIs) in rural PHC facilities in the Guangdong Province, China. A total of 444,979 outpatient prescriptions were extracted from the electronic medical record system of 35 township health centers (THCs) and 2 community health centers (CHCs) between November 2017 and October 2018. We used the chi-square test to analyze the antibiotic prescription patterns and binary logistic regression to explore patient-related factors associated with antibiotic prescriptions. Of the 162,742 ARI prescriptions, 85.57% (n = 139,259) included at least one antibiotic. Among the 139,259 prescriptions with antibiotics, 37.82% (n = 52,666) included two or more antibiotics, 55.29% (n = 76,993) included parenteral antibiotics, and 56.62% (n = 78,852) included Watch group antibiotics. The binary logistic regression indicated that (1) female patients were slightly less likely to be prescribed antibiotics than males (adjusted odds ratio (OR) = 0.954, 95% confidence interval [CI] [0.928–0.981]; p = 0.001); and (2) compared to patients aged ≤5 years, those who were 6–15 years old (adjusted OR = 1.907, 95% CI [1.840–1.978]; p < 0.001), 16–60 years old (adjusted OR = 1.849, 95% CI [1.785–1.916]; p < 0.001), and >60 years old (adjusted OR = 1.915, 95% CI [1.810–2.026]; p < 0.001) were more likely to be prescribed antibiotics. The overuse and irrational use of antibiotics in PHC settings remain major healthcare challenges in rural Guangdong. Thus, it is imperative to implement targeted antimicrobial stewardship (AMS) policies to address this problem.
Protective Effects of Dipterocarpus tuberculatus in Blue Light-Induced Macular Degeneration in A2E-Laden ARPE19 Cells and Retina of Balb/c Mice
Authors: Lee Su Jin , Roh Yu Jeong , Kim Ji Eun , Jin You Jeong , Song Hee Jin , Seol Ayun , Park So Hae , Douangdeuane Bounleuane , Souliya Onevilay , Choi Sun Il , Hwang Dae Youn
Published: February 2023

Natural products with significant antioxidant activity have been receiving attention as one of the treatment strategies to prevent age-related macular degeneration (AMD). Reactive oxygen intermediates (ROI) including oxo-N-retinylidene-N-retinylethanolamine (oxo-A2E) and singlet oxygen-induced damage, are believed to be one of the major causes of the development of AMD. To investigate the therapeutic effects of methanol extracts of Dipterocarpus tuberculatus Roxb. (MED) against blue light (BL)-caused macular degeneration, alterations in the antioxidant activity, apoptosis pathway, neovascularization, inflammatory response, and retinal degeneration were analyzed in A2E-laden ARPE19 cells and Balb/c mice after exposure of BL. Seven bioactive components, including 2α-hydroxyursolic acid, ε-viniferin, asiatic acid, bergenin, ellagic acid, gallic acid and oleanolic acid, were detected in MED. MED exhibited high DPPH and ABTS free radical scavenging activity. BL-induced increases in intracellular reactive oxygen species (ROS) production and nitric oxide (NO) concentration were suppressed by MED treatment. A significant recovery of antioxidant capacity by an increase in superoxide dismutase enzyme (SOD) activity, SOD expression levels, and nuclear factor erythroid 2–related factor 2 (NRF2) expression were detected as results of MED treatment effects. The activation of the apoptosis pathway, the expression of neovascular proteins, cyclooxygenase-2 (COX-2)-induced inducible nitric oxide synthase (iNOS) mediated pathway, inflammasome activation, and expression of inflammatory cytokines was remarkably inhibited in the MED treated group compared to the Vehicle-treated group in the AMD cell model. Furthermore, MED displayed protective effects in BL-induced retinal degeneration through improvement in the thickness of the whole retina, outer nuclear layer (ONL), inner nuclear layer (INL), and photoreceptor layer (PL) in Balb/c mice. Taken together, these results indicate that MED exhibits protective effects in BL-induced retinal degeneration and has the potential in the future to be developed as a treatment option for dry AMD with atrophy of retinal pigment epithelial (RPE) cells.