bluuebunny/crossref_metadata_2025 huggingface dataset:
Utilizing Nearest-Neighbor Clustering for Addressing Imbalanced Datasets in Bioengineering
Authors: Huang Chih-Ming , Lin Chun-Hung , Hung Chuan-Sheng , Zeng Wun-Hui , Zheng You-Cheng , Tsai Chih-Min
Published: March 2024

Imbalance classification is common in scenarios like fault diagnosis, intrusion detection, and medical diagnosis, where obtaining abnormal data is difficult. This article addresses a one-class problem, implementing and refining the One-Class Nearest-Neighbor (OCNN) algorithm. The original inter-quartile range mechanism is replaced with the K-means with outlier removal (KMOR) algorithm for efficient outlier identification in the target class. Parameters are optimized by treating these outliers as non-target-class samples. A new algorithm, the Location-based Nearest-Neighbor (LBNN) algorithm, clusters one-class training data using KMOR and calculates the farthest distance and percentile for each test data point to determine if it belongs to the target class. Experiments cover parameter studies, validation on eight standard imbalanced datasets from KEEL, and three applications on real medical imbalanced datasets. Results show superior performance in precision, recall, and G-means compared to traditional classification models, making it effective for handling imbalanced data challenges.
Stock Assessment of the Commercial Small Pelagic Fishes in the Beibu Gulf, the South China Sea, 2006–2020
Authors: Hong Xiaofan , Zhang Kui , Li Jiajun , Xu Youwei , Sun Mingshuai , Xu Shannan , Cai Yancong , Qiu Yongsong , Chen Zuozhi
Published: March 2024

Long-term variations in population structure, growth, mortality, exploitation rate, and recruitment pattern of two major commercial small pelagic fishes (CSPFs) (Decapterus maruadsi and Trachurus japonicus) are reported based on bottom trawl survey data collected during 2006–2020 in the Beibu Gulf, South China Sea. All individuals collected during each sampling quarter over a period of 15 years were subjected to laboratory-based analysis. In this study, the stock of D. maruadsi and T. japonicus inhabiting the Beibu Gulf was assessed using length-based methods (bootstrapped electronic length frequency analysis (ELEFAN)) to complete stock assessment in different fishery management periods (the division of fisheries management periods was based on China’s input and output in the South China Sea offshore fisheries over 15 years, specifically divided into period I (2006–2010), period II (2011–2015), and period III (2016–2020)). The results showed that the mean body length, dominant body size, and estimated asymptotic length of two CSPFs decreased, whereas their growth coefficient decreased, indicating miniaturization and slower growth, respectively. Estimated exploitation rates and catching body length for two CSPFs indicated that both stocks in the Beibu Gulf were overexploited in period I and moderately exploited after 2011. These stocks were taking a good turn in status in period III, with the exploitation rate much lower than the initial period and reversing the downward trend in catching body length. Furthermore, the variations in the spawning season of the two CSPF stocks and their barely satisfactory expected yield indicated the complexity of the current fishery management in the Beibu Gulf. These results suggest that management measures to reduce fishing pressure may have a positive influence on the biological characteristics of those CSPFs in the Beibu Gulf; however, the stock structure already affected by overfishing will be a huge challenge for the conservation and restoration of fisheries resources in the future. Given that the current stocks of D. maruadsi and T. japonicus in the Beibu Gulf still have low first-capture body length (Lc) and high fishing mortality (F) (compared to F0.1), we identify a need to refine population structure by controlling fishing efforts and increasing catchable size, and more consideration should be given to the local fishery resource status in fisheries management.
Allocation of Nutrients and Leaf Turnover Rate in Poplar under Ambient and Enriched Ozone Exposure and Soil Nutrient Manipulation
Authors: Paoletti Elena , Pagano Mario , Zhang Lu , Badea Ovidiu , Hoshika Yasutomo
Published: March 2024

An excess of ozone (O3) is currently stressing plant ecosystems and may negatively affect the nutrient use of plants. Plants may modify leaf turnover rates and nutrient allocation at the organ level to counteract O3 damage. We investigated leaf turnover rate and allocation of primary (C, N, P, K) and secondary macronutrients (Ca, S, Mg) under various O3 treatments (ambient concentration, AA, with a daily hourly average of 35 ppb; 1.5 × AA; 2.0 × AA) and fertilization levels (N: 0 and 80 kg N ha−1 y−1; P: 0 and 80 kg N ha−1 y−1) in an O3-sensitive poplar clone (Oxford: Populus maximowiczii Henry × P. berolinensis Dippel) in a Free-Air Controlled Exposure (FACE) experiment. The results indicated that both fertilization and O3 had a significant impact on the nutrient content. Specifically, fertilization and O3 increased foliar C and N contents (+5.8% and +34.2%, respectively) and root Ca and Mg contents (+46.3% and +70.2%, respectively). Plants are known to increase the content of certain elements to mitigate the damage caused by high levels of O3. The leaf turnover rate was accelerated as a result of increased O3 exposure, indicating that O3 plays a main role in influencing this physiological parameter. A PCA result showed that O3 fumigation affected the overall allocation of primary and secondary elements depending on the organ (leaves, stems, roots). As a conclusion, such different patterns of element allocation in plant leaves in response to elevated O3 levels can have significant ecological implications.
First Insights into Body Localization of an Osmoregulation-Related Cotransporter in Estuarine Annelids
Authors: Mucciolo Serena , Desiderato Andrea , Mastrodonato Maria , Lana Paulo , Arruda Freire Carolina , Prodocimo Viviane
Published: March 2024

The expression of the Na+-K+-2Cl− cotransporter (NKCC), widely associated with cell volume regulation, has never been directly demonstrated in annelids. Its putative presence was firstly recovered in silico, and then using immunofluorescence, its signal was retrieved for the first time in different tissues of four species of estuarine annelids from southern Brazil that are regularly subjected to salinity fluctuations. We tested two euryhaline species (wide salinity tolerance), the nereidids Alitta yarae and Laeonereis acuta (habitat salinity: ~10–28 psu), and two stenohaline species (restricted salinity tolerance), the nephtyid Nephtys fluviatilis (habitat salinity: ~6–10 psu), and the melinnid Isolda pulchella (habitat salinity: ~28–35 psu). All four species showed specific immunofluorescent labelling for NKCC-like expression. However, the expression of an NKCC-like protein was not homogeneous among them. The free-living/burrowers (both euryhaline nereidids and the stenohaline nephtyid) displayed a widespread signal for an NKCC-like protein along their bodies, in contrast to the stenohaline sedentary melinnid, in which the signal was restricted to the branchiae and the internal tissues of the body. The results are compatible with NKCC involvement in cell volume, especially in annelids that face wide variations in salinity in their habitats.
(Pentamethylcyclopentadienyl)chloridoiridium(III) Complex Bearing Bidentate Ph2PCH2CH2SPh-κP,κS Ligand
Authors: Ludwig Gerd , Ranđelović Ivan , Dimić Dušan , Komazec Teodora , Maksimović-Ivanić Danijela , Mijatović Sanja , Rüffer Tobias , Kaluđerović Goran N.
Published: March 2024

The (pentamethylcyclopentadienyl)chloridoiridium(III) complex bearing a κP,κS-bonded Ph2PCH2CH2SPh ligand ([Ir(η5-C5Me5)Cl(Ph2P(CH2)2SPh-κP,κS)]PF6, (1)] was synthesized and characterized. Multinuclear (1H, 13C and 31P) NMR spectroscopy was employed for the determination of the structure. Moreover, SC-XRD confirmed the proposed structure belongs to the “piano stool” type. The Hirshfeld surface analysis outlined the most important intermolecular interactions in the structure. The crystallographic structure was optimized at the B3LYP-D3BJ/6-311++G(d,p)(H,C,P,S,Cl)/LanL2DZ(Ir) level of theory. The applicability of this level was verified through a comparison of experimental and theoretical bond lengths and angles, and 1H and 13C NMR chemical shifts. The Natural Bond Orbital theory was used to identify and quantify the intramolecular stabilization interactions, especially those between donor atoms and Ir(III) ions. Complex 1 was tested on antitumor activity against five human tumor cell lines: MCF-7 breast adenocarcinoma, SW480 colon adenocarcinoma, 518A2 melanoma, 8505C human thyroid carcinoma and A253 submandibular carcinoma. Complex 1 showed superior antitumor activity against cisplatin-resistant MCF-7, SW480 and 8505C cell lines. The mechanism of tumoricidal action on 8505C cells indicates the involvement of caspase-induced apoptosis, accompanied by a considerable reduction in ROS/RNS and proliferation potential of treated cells.
Changes in Internal Structure and Dynamics upon Binding Stabilise the Nematode Anticoagulant NAPc2
Authors: Woodward Elaine , Duggan Brendan M.
Published: March 2024

Abnormal blood coagulation is a major health problem and natural anticoagulants from blood-feeding organisms have been investigated as novel therapeutics. NAPc2, a potent nematode-derived inhibitor of coagulation, has an unusual mode of action that requires coagulation factor Xa but does not inhibit it. Molecular dynamics simulations of NAPc2 and factor Xa were generated to better understand NAPc2. The simulations suggest that parts of NAPc2 become more rigid upon binding factor Xa and reveal that two highly conserved residues form an internal salt bridge that stabilises the bound conformation. Clotting time assays with mutants confirmed the utility of the salt bridge and suggested that it is a conserved mechanism for stabilising the bound conformation of secondary structure-poor protease inhibitors.
The Role of BDNF and TrkB in the Central Control of Energy and Glucose Balance: An Update
Authors: Harvey Theresa , Rios Maribel
Published: March 2024

The global rise in obesity and related health issues, such as type 2 diabetes and cardiovascular disease, is alarming. Gaining a deeper insight into the central neural pathways and mechanisms that regulate energy and glucose homeostasis is crucial for developing effective interventions to combat this debilitating condition. A significant body of evidence from studies in humans and rodents indicates that brain-derived neurotrophic factor (BDNF) signaling plays a key role in regulating feeding, energy expenditure, and glycemic control. BDNF is a highly conserved neurotrophin that signals via the tropomyosin-related kinase B (TrkB) receptor to facilitate neuronal survival, differentiation, and synaptic plasticity and function. Recent studies have shed light on the mechanisms through which BDNF influences energy and glucose balance. This review will cover our current understanding of the brain regions, neural circuits, and cellular and molecular mechanisms underlying the metabolic actions of BDNF and TrkB.
Diabetic Cardiomyopathy—From Basics through Diagnosis to Treatment
Authors: Radzioch Ewa , Dąbek Bartłomiej , Balcerczyk-Lis Marta , Frąk Weronika , Fularski Piotr , Młynarska Ewelina , Rysz Jacek , Franczyk Beata
Published: March 2024

Diabetic cardiomyopathy (DCM) is the development of myocardial dysfunction in patients with diabetes despite the absence of comorbidities such as hypertension, atherosclerosis or valvular defect. The cardiovascular complications of poorly controlled diabetes are very well illustrated by the U.K. Prospective Diabetes Study (UKPDS), which showed a clear association between increasing levels of glycated hemoglobin and the development of heart failure (HF). The incidence of HF in patients with diabetes is projected to increase significantly, which is why its proper diagnosis and treatment is so important. Providing appropriate therapy focusing on antidiabetic and hypolipemic treatment with the consideration of pharmacotherapy for heart failure reduces the risk of CMD and reduces the incidence of cardiovascular complications. Health-promoting changes made by patients such as a low-carbohydrate diet, regular exercise and weight reduction also appear to be important in achieving appropriate outcomes. New hope for the development of therapies for DCM is offered by novel methods using stem cells and miRNA, which, however, require more thorough research to confirm their efficacy.
Elevated NET, Calprotectin, and Neopterin Levels Discriminate between Disease Activity in COVID-19, as Evidenced by Need for Hospitalization among Patients in Northern Italy
Authors: Hetland Geir , Fagerhol Magne Kristoffer , Mirlashari Mohammad Reza , Nissen-Meyer Lise Sofie Haug , Croci Stefania , Lonati Paola Adele , Bonacini Martina , Salvarani Carlo , Marvisi Chiara , Bodio Caterina , Muratore Francesco , Borghi Maria Orietta , Meroni Pier Luigi
Published: March 2024

Coronavirus disease 2019 (COVID-19) displays clinical heterogeneity, but little information is available for patients with mild or very early disease. We aimed to characterize biomarkers that are useful for discriminating the hospitalization risk in a COVID-19 cohort from Northern Italy during the first pandemic wave. We enrolled and followed for four weeks 76 symptomatic SARS-CoV-2 positive patients and age/sex-matched healthy controls. Patients with mild disease were discharged (n.42), and the remaining patients were hospitalized (n.34). Blood was collected before any anti-inflammatory/immunosuppressive therapy and assessed for soluble C5b-9/C5a, H3-neutrophil extracellular traps (NETs), calprotectin, and DNase plasma levels via ELISA and a panel of proinflammatory cytokines via ELLA. Calprotectin and NET levels discriminate between hospitalized and non-hospitalized patients, while DNase negatively correlates with NET levels; there are positive correlations between calprotectin and both NET and neopterin levels. Neopterin levels increase in patients at the beginning of the disease and do so more in hospitalized than non-hospitalized patients. C5a and sC5b-9, and other acute phase proteins, correlate with neopterin, calprotectin, and DNase. Both NET and neopterin levels negatively correlate with platelet count. We show that calprotectin, NETs, and neopterin are important proinflammatory parameters potentially useful for discriminating between COVID-19 patients at risk of hospitalization.
Vitamin D and Cardiovascular Diseases: From Physiology to Pathophysiology and Outcomes
Authors: Nardin Matteo , Verdoia Monica , Nardin Simone , Cao Davide , Chiarito Mauro , Kedhi Elvin , Galasso Gennaro , Condorelli Gianluigi , De Luca Giuseppe
Published: March 2024

Vitamin D is rightly recognized as an essential key factor in the regulation of calcium and phosphate homeostasis, affecting primary adequate bone mineralization. In the last decades, a more complex and wider role of vitamin D has been postulated and demonstrated. Cardiovascular diseases have been found to be strongly related to vitamin D levels, especially to its deficiency. Pre-clinical studies have suggested a direct role of vitamin D in the regulation of several pathophysiological pathways, such as endothelial dysfunction and platelet aggregation; moreover, observational data have confirmed the relationship with different conditions, including coronary artery disease, heart failure, and hypertension. Despite the significant evidence available so far, most clinical trials have failed to prove any positive impact of vitamin D supplements on cardiovascular outcomes. This discrepancy indicates the need for further information and knowledge about vitamin D metabolism and its effect on the cardiovascular system, in order to identify those patients who would benefit from vitamin D supplementation.
Immunolocalization of Matrix Metalloproteinases 2 and 9 and Their Inhibitors in the Hearts of Rats Treated with Immunosuppressive Drugs—An Artificial Intelligence-Based Digital Analysis
Authors: Wilk Aleksandra , Król Małgorzata , Kiełbowski Kajetan , Bakinowska Estera , Szumilas Kamila , Surówka Anna , Kędzierska-Kapuza Karolina
Published: March 2024

Background: Immunosuppressive agents represent a broad group of drugs, such as calcineurin inhibitors, mTOR inhibitors, and glucocorticosteroids, among others. These drugs are widely used in a number of conditions, but lifelong therapy is crucial in the case of organ recipients to prevent rejection. To further increase the safety and efficacy of these agents, their off-target mechanisms of action, as well as processes underlying the pathogenesis of adverse effects, need to be thoroughly investigated. The aim of this study was to examine the impact of various combinations of cyclosporine/tacrolimus/mycophenolate with rapamycin and steroids (CRG, TRG, MRG), on the morphology and morphometry of rats’ cardiomyocytes, together with the presence of cardiac collagen and the immunoexpression of MMPs and TIMPs. Methods: Twenty-four rats were divided into four groups receiving different immunosuppressive regiments. After six months of treatment, the hearts were collected and analyzed. Results: Cardiomyocytes from the CRG cohorts demonstrated the most pronounced morphological alterations. In addition, chronic immunosuppression reduced the width and length of cardiac cells. However, immunosuppressive therapy did not alter the presence of cardiac collagen fibers. Nevertheless, we observed significant alterations regarding MMP/TIMP homeostasis. Conclusions: Chronic immunosuppression seems to disturb the MMP/TIMP balance in aspects of immunolocalization in the hearts of rats. Further studies are required to analyze other mechanisms and pathways affected by the use of immunosuppressants.
Cellular and Molecular Mechanisms of Heterotopic Ossification in Fibrodysplasia Ossificans Progressiva
Authors: Mejias Rivera Loreilys , Shore Eileen M. , Mourkioti Foteini
Published: April 2024

Fibrodysplasia ossificans progressiva (FOP) is a debilitating genetic disorder characterized by recurrent episodes of heterotopic ossification (HO) formation in muscles, tendons, and ligaments. FOP is caused by a missense mutation in the ACVR1 gene (activin A receptor type I), an important signaling receptor involved in endochondral ossification. The ACVR1R206H mutation induces increased downstream canonical SMAD-signaling and drives tissue-resident progenitor cells with osteogenic potential to participate in endochondral HO formation. In this article, we review aberrant ACVR1R206H signaling and the cells that give rise to HO in FOP. FOP mouse models and lineage tracing analyses have been used to provide strong evidence for tissue-resident mesenchymal cells as cellular contributors to HO. We assess how the underlying mutation in FOP disrupts muscle-specific dynamics during homeostasis and repair, with a focus on muscle-resident mesenchymal cells known as fibro-adipogenic progenitors (FAPs). Accumulating research points to FAPs as a prominent HO progenitor population, with ACVR1R206H FAPs not only aberrantly differentiating into chondro-osteogenic lineages but creating a permissive environment for bone formation at the expense of muscle regeneration. We will further discuss the emerging role of ACVR1R206H FAPs in muscle regeneration and therapeutic targeting of these cells to reduce HO formation in FOP.
Bioinspired and Multifunctional Tribological Materials for Sliding, Erosive, Machining, and Energy-Absorbing Conditions: A Review
Authors: Kumar Rahul , Rezapourian Mansoureh , Rahmani Ramin , Maurya Himanshu S. , Kamboj Nikhil , Hussainova Irina
Published: March 2024

Friction, wear, and the consequent energy dissipation pose significant challenges in systems with moving components, spanning various domains, including nanoelectromechanical systems (NEMS/MEMS) and bio-MEMS (microrobots), hip prostheses (biomaterials), offshore wind and hydro turbines, space vehicles, solar mirrors for photovoltaics, triboelectric generators, etc. Nature-inspired bionic surfaces offer valuable examples of effective texturing strategies, encompassing various geometric and topological approaches tailored to mitigate frictional effects and related functionalities in various scenarios. By employing biomimetic surface modifications, for example, roughness tailoring, multifunctionality of the system can be generated to efficiently reduce friction and wear, enhance load-bearing capacity, improve self-adaptiveness in different environments, improve chemical interactions, facilitate biological interactions, etc. However, the full potential of bioinspired texturing remains untapped due to the limited mechanistic understanding of functional aspects in tribological/biotribological settings. The current review extends to surface engineering and provides a comprehensive and critical assessment of bioinspired texturing that exhibits sustainable synergy between tribology and biology. The successful evolving examples from nature for surface/tribological solutions that can efficiently solve complex tribological problems in both dry and lubricated contact situations are comprehensively discussed. The review encompasses four major wear conditions: sliding, solid-particle erosion, machining or cutting, and impact (energy absorbing). Furthermore, it explores how topographies and their design parameters can provide tailored responses (multifunctionality) under specified tribological conditions. Additionally, an interdisciplinary perspective on the future potential of bioinspired materials and structures with enhanced wear resistance is presented.
Design and Evaluation of a Bilateral Semi-Rigid Exoskeleton to Assist Hip Motion
Authors: Mohammadzadeh Gonabadi Arash , Antonellis Prokopios , Dzewaltowski Alex C. , Myers Sara A. , Pipinos Iraklis I. , Malcolm Philippe
Published: March 2024

This study focused on designing and evaluating a bilateral semi-rigid hip exoskeleton. The exoskeleton assisted the hip joint, capitalizing on its proximity to the body’s center of mass. Unlike its rigid counterparts, the semi-rigid design permitted greater freedom of movement. A temporal force-tracking controller allowed us to prescribe torque profiles during walking. We ensured high accuracy by tuning control parameters and series elasticity. The evaluation involved experiments with ten participants across ten force profile conditions with different end-timings and peak magnitudes. Our findings revealed a trend of greater reductions in metabolic cost with assistance provided at later timings in stride and at greater magnitudes. Compared to walking with the exoskeleton powered off, the largest reduction in metabolic cost was 9.1%. This was achieved when providing assistance using an end-timing at 44.6% of the stride cycle and a peak magnitude of 0.11 Nm kg−1. None of the tested conditions reduced the metabolic cost compared to walking without the exoskeleton, highlighting the necessity for further enhancements, such as a lighter and more form-fitting design. The optimal end-timing aligns with findings from other soft hip exosuit devices, indicating a comparable interaction with this prototype to that observed in entirely soft exosuit prototypes.
Enzyme Immobilization by Inkjet Printing on Reagentless Biosensors for Electrochemical Phosphate Detection
Authors: Zhang Dongxing , Bai Yang , Niu Haoran , Chen Lingyun , Xiao Junfeng , Guo Qiuquan , Jia Peipei
Published: March 2024

Enzyme-based biosensors commonly utilize the drop-casting method for their surface modification. However, the drawbacks of this technique, such as low reproducibility, coffee ring effects, and challenges in mass production, hinder its application. To overcome these limitations, we propose a novel surface functionalization strategy of enzyme crosslinking via inkjet printing for reagentless enzyme-based biosensors. This method includes printing three functional layers onto a screen-printed electrode: the enzyme layer, crosslinking layer, and protective layer. Nanomaterials and substrates are preloaded together during our inkjet printing. Inkjet-printed electrodes feature a uniform enzyme deposition, ensuring high reproducibility and superior electrochemical performance compared to traditional drop-casted ones. The resultant biosensors display high sensitivity, as well as a broad linear response in the physiological range of the serum phosphate. This enzyme crosslinking method has the potential to extend into various enzyme-based biosensors through altering functional layer components.
Novel Pseudomonas Species Prevent the Growth of the Phytopathogenic Fungus Aspergillus flavus
Authors: Rabiço Franciene , Borelli Tiago Cabral , Alnoch Robson Carlos , Polizeli Maria de Lourdes Teixeira de Moraes , da Silva Ricardo R. , Silva-Rocha Rafael , Guazzaroni María-Eugenia
Published: March 2024

In response to the escalating demand for sustainable agricultural methodologies, the utilization of microbial volatile organic compounds (VOCs) as antagonists against phytopathogens has emerged as a viable eco-friendly alternative. Microbial volatiles exhibit rapid diffusion rates, facilitating prompt chemical interactions. Moreover, microorganisms possess the capacity to emit volatiles constitutively, as well as in response to biological interactions and environmental stimuli. In addition to volatile compounds, these bacteria demonstrate the ability to produce soluble metabolites with antifungal properties, such as APE Vf, pyoverdin, and fragin. In this study, we identified two Pseudomonas strains (BJa3 and MCal1) capable of inhibiting the in vitro mycelial growth of the phytopathogenic fungus Aspergillus flavus, which serves as the causal agent of diseases in sugarcane and maize. Utilizing GC/MS analysis, we detected 47 distinct VOCs which were produced by these bacterial strains. Notably, certain volatile compounds, including 1-heptoxydecane and tridecan-2-one, emerged as primary candidates for inhibiting fungal growth. These compounds belong to essential chemical classes previously documented for their antifungal activity, while others represent novel molecules. Furthermore, examination via confocal microscopy unveiled significant morphological alterations, particularly in the cell wall, of mycelia exposed to VOCs emitted by both Pseudomonas species. These findings underscore the potential of the identified BJa3 and MCal1 Pseudomonas strains as promising agents for fungal biocontrol in agricultural crops.
Concordance between Wada, Transcranial Magnetic Stimulation, and Magnetoencephalography for Determining Hemispheric Dominance for Language: A Retrospective Study
Authors: Noorizadeh Negar , Rezaie Roozbeh , Varner Jackie A. , Wheless James W. , Fulton Stephen P. , Mudigoudar Basanagoud D. , Nevill Leigh , Holder Christen M. , Narayana Shalini
Published: March 2024

Determination of language hemispheric dominance (HD) in patients undergoing evaluation for epilepsy surgery has traditionally relied on the sodium amobarbital (Wada) test. The emergence of non-invasive methods for determining language laterality has increasingly shown to be a viable alternative. In this study, we assessed the efficacy of transcranial magnetic stimulation (TMS) and magnetoencephalography (MEG), compared to the Wada test, in determining language HD in a sample of 12 patients. TMS-induced speech errors were classified as speech arrest, semantic, or performance errors, and the HD was based on the total number of errors in each hemisphere with equal weighting of all errors (classic) and with a higher weighting of speech arrests and semantic errors (weighted). Using MEG, HD for language was based on the spatial extent of long-latency activity sources localized to receptive language regions. Based on the classic and weighted language laterality index (LI) in 12 patients, TMS was concordant with the Wada in 58.33% and 66.67% of patients, respectively. In eight patients, MEG language mapping was deemed conclusive, with a concordance rate of 75% with the Wada test. Our results indicate that TMS and MEG have moderate and strong agreement, respectively, with the Wada test, suggesting they could be used as non-invasive substitutes.
Selective Alteration of the Left Arcuate Fasciculus in Two Patients Affected by Creatine Transporter Deficiency
Authors: Balestrino Maurizio , Adriano Enrico , Alì Paolo Alessandro , Pardini Matteo
Published: March 2024

(1) Background: In hereditary creatine transporter deficiency (CTD), there is an absence of creatine in the brain and neurological symptoms are present, including severe language impairment. However, the pathological changes caused by creatine deficiency that generate neuropsychological symptoms have been poorly studied. (2) Aims: To investigate if the language impairment in CTD is underpinned by possible pathological changes. (3) Methods: We used MRI tractography to investigate the trophism of the left arcuate fasciculus, a white matter bundle connecting Wernicke’s and Broca’s language areas that is specifically relevant for language establishment and maintenance, in two patients (28 and 18 y.o.). (4) Results: The T1 and T2 MRI imaging results were unremarkable, but the left arcuate fasciculus showed a marked decrease in mean fractional anisotropy (FA) compared to healthy controls. In contrast, the FA values in the corticospinal tract were similar to those of healthy controls. Although white matter atrophy has been reported in CTD, this is the first report to show a selective abnormality of the language-relevant arcuate fasciculus, suggesting a possible region-specific impact of creatine deficiency.
Impact of Benzodiazepines and Illness Duration on Obsessive–Compulsive Disorder during COVID-19 in Italy: Exploring Symptoms’ Evolutionary Benefits
Authors: D’Urso Giordano , Magliacano Alfonso , Manzo Marco , Pomes Mattia Vittorio , Iuliano Carla , Iasevoli Felice , Dell’Osso Bernardo , de Bartolomeis Andrea
Published: March 2024

Obsessive–compulsive disorder (OCD) is believed to follow a waxing and waning course, often according to environmental stressors. During the COVID-19 pandemic, pre-existing OCD symptoms were reported to increase and to change from checking to washing behaviors, while new-onset symptoms were predominantly of the hoarding type. In the present study, we followed the evolution of OCD symptoms, anxiety, depression, and insights of illness in forty-six OCD patients throughout the pandemic. Clinical measures were collected at four different time points before and during the COVID-19 pandemic in Italy. Within-subject comparisons were used to compare clinical scale scores across time, and correlations were examined between patients’ baseline characteristics and changes in clinical scores. We found that all clinical measures increased during the first Italian lockdown with respect to the pre-pandemic values. Anxiety decreased during the temporary elimination of restriction provisions, whereas the severity of OCD symptoms and insight returned to pre-pandemic values during the second mandatory lockdown. These results were observed only in two sub-groups of patients: those taking benzodiazepines and those with shorter illness duration. Our findings suggest the need for additional clinical attention to these specific sub-groups of OCD patients in case of particularly distressing circumstances while pointing to a possible adaptive role of their OCD symptoms when the environment requires a higher care of hygiene and an extraordinary supply of essential resources.
Use of Prescribed and Non-Prescribed Treatments for Cluster Headache in a Swedish Cohort
Authors: Smedfors Gabriella , Jennysdotter Olofsgård Felicia , Steinberg Anna , Waldenlind Elisabet , Ran Caroline , Belin Andrea Carmine
Published: March 2024

Background: Cluster headache (CH) is a debilitating condition, but current therapies leave CH patients in pain. The extent of this problem in Sweden is unknown. Methods: An anonymized questionnaire was sent to 479 Swedish CH patients to investigate patterns and perceived effects of treatments. Results: Three hundred fourteen answers were analyzed. The population was representative regarding age of onset and sex. Less than half (46%) were satisfied with their abortive treatments, 19% terminated functioning abortive treatments due to side effects. Additionally, 17% of chronic CH patients had not tried the first-line preventive drug verapamil. A small subset had tried illicit substances to treat their CH (0–8% depending on substance). Notably, psilocybin was reported effective as an abortive treatment by 100% (n = 8), and with some level of effect as a preventive treatment by 92% (n = 12). For verapamil, some level of preventive effect was reported among 68% (n = 85). Conclusions: Our descriptive data illustrate that many Swedish CH patients are undertreated, lack functional therapies, and experience side effects. Further studies are warranted to search for new treatment strategies as well as a revision of current treatment guidelines with the aim of reducing patient disease burden to the greatest extent possible.
Investigating Dyslexia through Diffusion Tensor Imaging across Ages: A Systematic Review
Authors: Martins Bruce , Baba Mariana Yumi , Dimateo Elisa Monteiro , Costa Leticia Fruchi , Camara Aila Silveira , Lukasova Katerina , Nucci Mariana Penteado
Published: March 2024

Dyslexia is a neurodevelopmental disorder that presents a deficit in accuracy and/or fluency while reading or spelling that is not expected given the level of cognitive functioning. Research indicates brain structural changes mainly in the left hemisphere, comprising arcuate fasciculus (AF) and corona radiata (CR). The purpose of this systematic review is to better understand the possible methods for analyzing Diffusion Tensor Imaging (DTI) data while accounting for the characteristics of dyslexia in the last decade of the literature. Among 124 articles screened from PubMed and Scopus, 49 met inclusion criteria, focusing on dyslexia without neurological or psychiatric comorbidities. Article selection involved paired evaluation, with a third reviewer resolving discrepancies. The selected articles were analyzed using two topics: (1) a demographic and cognitive assessment of the sample and (2) DTI acquisition and analysis. Predominantly, studies centered on English-speaking children with reading difficulties, with preserved non-verbal intelligence, attention, and memory, and deficits in reading tests, rapid automatic naming, and phonological awareness. Structural differences were found mainly in the left AF in all ages and in the bilateral superior longitudinal fasciculus for readers-children and adults. A better understanding of structural brain changes of dyslexia and neuroadaptations can be a guide for future interventions.
Sense of Agency and Skills Learning in Virtual-Mediated Environment: A Systematic Review
Authors: Cesari Valentina , D’Aversa Sveva , Piarulli Andrea , Melfi Franca , Gemignani Angelo , Menicucci Danilo
Published: March 2024

Agency is central to remote actions, and it may enhance skills learning due to a partial overlap between brain structures and networks, the promotion of confidence towards a telemanipulator, and the feeling of congruence of the motor choice to the motor plan. We systematically reviewed studies aiming to verify the role of agency in improving learning. Fifteen studies were selected from MEDLINE and Scopus®. When a mismatch is introduced between observed and performed actions, the decrease in agency and learning is proportional to the intensity of the mismatch, which is due to greater interference with the motor programming. Thanks to multisensory integration, agency and learning benefit both from sensory and performance feedback and from the timing of feedback based on control at the goal level or the perceptual–motor level. This work constitutes a bedrock for professional teleoperation settings (e.g., robotic surgery), with particular reference to the role of agency in performing complex tasks with remote control.
Different but Synergistic Effects of Union and Manager Leadership on Member Job Satisfaction
Authors: Jung Heungjun , Kim Ki-Jung , Shin Inyong
Published: March 2024

Existing research has tended to overlook the diverse roles of union leadership in contributing to member attitudes. Drawing on the social information processing theory, this study examines how union leaders’ (shop stewards) service-oriented leadership relates to member job satisfaction. To clarify the mechanism underlying this relationship, this study focuses on union instrumentality as a mediator. The research also examines managers’ ethical leadership as a conditional factor in the relationship between union leaders’ service-oriented leadership and member job satisfaction through union instrumentality. To test our hypothesis, this study analyzed the results of a survey of 603 respondents from two branches of the Korean Metal Workers’ Union. The findings of this study indicate that union instrumentality is the link between service-oriented union leadership and member job satisfaction. Additionally, the strength of the mediated relationship between the aforementioned factors through union instrumentality is contingent on managerial ethical leadership. This study contributes to an integrated understanding of the way in which service-oriented union stewards and ethical managers influence member job satisfaction through their leadership.
How Is Job Insecurity Related to Workers’ Work–Family Conflict during the Pandemic? The Mediating Role of Working Excessively and Techno-Overload
Authors: Finstad Georgia Libera , Bernuzzi Chiara , Setti Ilaria , Fiabane Elena , Giorgi Gabriele , Sommovigo Valentina
Published: March 2024

The current labor market is characterized by drastic changes linked to the use of information and communication technologies (ICT) and post-COVID-19 transformations, which have decreased job security and job stability. As a result, the feeling of losing one’s job has become even more common among European workers. In this study, we aimed to investigate whether and how job insecurity would be related to work–family conflict during the pandemic. Online self-report questionnaires assessing job insecurity, working excessively, techno-overload, and work-to-family conflict were completed by 266 workers from Italy. Descriptive analyses, confirmatory factor analyses, and structural equation mediation models were conducted. Job insecurity was positively associated with work-to-family conflict, both directly and indirectly, as mediated by techno-overload and a tendency to work excessively. This study advances the literature, as it is the first to identify techno-overload and working excessively as parallel psychological mechanisms linking job insecurity to work–family conflict among Italian workers during the pandemic. Workers could benefit from technological workload monitoring programs, techno effectiveness, and time management training programs. Companies could also consider implementing family-friendly and digital disconnection practices.
Electroencephalography (EEG) Evidence for the Psychological Processes of Humor Generation: A Comparison Perspective on Humor and Creativity
Authors: Sun Cuicui , Zhou Zhijin
Published: March 2024

(1) Background: Humor stands out as the most dynamic and innovative aspect of human intelligence. Drawing on the cognitive parallels between humor and creativity, this study explored the EEG alpha frequency band activity patterns during humor generation by comparing the process of generating humorous and creative ideas. (2) Methods: Thirty-six participants were randomly assigned to either the humor generation group or the creative generation group, and the dependent variable was the neural oscillation in both low-frequency and high-frequency alpha during the early, middle, and late stages of both humor and creative generation. (3) Results: In the early stages, both humor and creative generation exhibited significantly higher power in low-frequency alpha and high-frequency alpha in the temporal region compared to the middle and late stages. In the middle and late stages, the low-frequency alpha oscillation in the frontal region for humor generation was significantly higher than that for creative generation. (4) Conclusions: Humor and creative generation share similar neural activation patterns in the early stages, involving the activation and retrieval of long-term memory information based on contextual cues. The differences between the two primarily manifest in the middle and late stages, where the selection of humorous ideas requires inhibiting not only irrelevant or ordinary ideas, akin to creative generation but also novel yet non-humorous ideas. This study sheds light on the neurocognitive mechanisms of humor generation and provides insights into the cognitive parallels and distinctions between humor generation and creative generation.
Evaluation of Eco-Friendly Consolidating Treatments in Pugliese Tuff (Gravina Calcarenite) Used in Italian Heritage Buildings
Authors: Huesca-Tortosa Jose Antonio , Spairani-Berrio Yolanda , Coviello Cristiano Giuseppe , Sabbà Maria Francesca , Rizzo Fabio , Foti Dora
Published: March 2024

This work evaluates the effectiveness of various consolidating treatments applied to Pugliese tuff (Gravina Calcarenite). This type of stone has been used in numerous historic buildings in the Puglia area (southeast of Italy), which presents durability problems due to high porosity, low cohesion between clasts, and low mechanical resistance. Eco-friendly treatments that generate CaCO3 have been selected, specifically bioconsolidant KBYO biological and lime water, which a priori are capable of consolidating without occluding the pores or reducing them excessively, thereby creating compounds similar to those contained in the stone and being respectful of the environment. Nano-sized treatments have also been tested, including nanosilica and nanolime, to compare results with eco-friendly treatments. The bioconsolidating treatment has been applied in two different ways, the usual way consisting of two applications a day for 7 days, as well as a double treatment that is applied in two batches of 7 days with a rest of 7 days between applications. Double treatment has shown a great improvement in consolidation compared to the usual 7-day application; this treatment has obtained the best results in both mechanical and petrophysical properties. This study not only demonstrates the effectiveness of the bioconsolidant but also expands eco-friendly conservation strategies to improve the preservation of historical structures built in calcarenite.
Flow Pattern and Escape Hazards of People from Flood Intrusion into the Staircase of Underground Spaces with Multiple Rest Platforms
Authors: Lin Zhiyu , Hu Shengbin , Lin Hang
Published: March 2024

While urban underground space is being built and developed at a high speed, urban flooding is also occurring gradually and frequently. Urban water, in many disasters, has intruded into underground spaces, such as subway stations, often leading to serious casualties, in which it is crucial for people to be able to escape from the staircases. In order to enable and guide the escape of people in underground floods, a staircase model with multiple rest platforms, applicable to common entrance and exit staircase forms, was constructed. The realizable k-ε turbulence model, coupled with a volume of fluid (VOF) method, was used to simulate and analyze the flow patterns when floods of various heights intrude into the structure. The effects of rest platform settings on the ejection phenomena and flow velocity changes in flood flows were summarized. The change rule of flood flow velocity on the stairs under different flood heights and stair heights was summarized, and a linear relationship between the peak flood flow velocity and the location of the peak flow velocity point on each flight of stairs was derived. Combined with the formula of the critical conditions for people to escape upwards in the flood, the proposed escape conditions for staircases with multiple rest platforms were proposed, which provide a basis for guiding the evacuation of people in times of disaster.
Environmental and Economic Assessment of Energy Renovation in Buildings, a Case Study in Greece
Authors: Dragonetti Lorna , Papadaki Dimitra , Assimakopoulos Margarita-Niki , Ferrante Annarita , Iannantuono Marco
Published: March 2024

The environmental and economic evaluation of energy renovation in buildings plays a crucial role in achieving sustainability goals and the decarbonization of the built environment. This paper presents a case study of a student house in Athens, Greece, to assess the environmental and economic impacts of energy renovation and seismic reinforcement with a steel exoskeleton. This study utilizes a comprehensive approach that combines life cycle assessment (LCA) and life cycle costing (LCC) methodologies using One Click LCA. The LCA assesses the environmental impacts associated with energy consumption and greenhouse gas emissions, while the LCC evaluates the economic aspects, both analyses being conducted for a lifespan of 25 years from now. The results provide an evaluation of what would happen in terms of greenhouse emissions and costs in two scenarios: with and without interventions. ProGETonE strategy results in an environmental impact with a GWP of 26.78 kgCO2eq/m2y with a reduction of 30% of the pre-renovation state. Economically, the actualized energy use costs for 25 years are 50% less in the post-renovation state, but the high construction costs make the strategy seem inconvenient. In this context, it is important to consider the non-economic benefits of seismic reinforcement, such as enhanced safety and the potential lives saved, which are critical in high seismic zones. These advantages complement the strategy’s environmental and energy use impacts, underscoring the holistic value of integrated seismic and energy retrofitting approaches like ProGETonE. The study underscores the importance of LCA and LCC analyses when evaluating the feasibility of renovation projects and of an evidence-based decision-making process for policymakers, building owners, and stakeholders for energy-efficient retrofitting.
Factors Impacting Women Gaining Leadership Roles in the Jordanian Construction Sector: Architects and Civil Engineers
Authors: Alshdiefat Ala’a Saleh , Sharif Ahlam Ammar , Abu Ghunmi Noor-Alhuda Mohammad , Lee Angela , Rana Muhammad Qasim
Published: March 2024

The persistent underrepresentation of women in leadership positions within the construction industry remains a global concern. In Jordan, despite comprising 60.45% and 22.4% of the total workforce of architects and civil engineers, respectively, women’s contribution to management roles is significantly low. Therefore, there is an urgent necessity to examine the factors hindering women’s advancement in the construction sector and their ability to attain leadership positions. This research aims to provide an overview of the current situation in Jordan, focusing specifically on the architectural and civil engineering professions. It presents findings from a desktop study, a survey questionnaire, and focus groups. The Severity Index (SI) formula is utilised to identify critical barriers in the Jordanian context, derived from both the literature review and questionnaire responses. Additionally, the Interpretive Structural Modelling (ISM) technique is employed to establish a hierarchy of critical barriers and analyse their interrelationships. The study reveals that the obstacles impeding women from assuming leadership roles in the Jordanian construction sector primarily stem from 20 critical barriers categorised across 11 levels in ISM. Notably, the lack of childcare programmes is identified as a fundamental barrier at the lower level, while informal networks formed by men emerge as the highest-rated barrier at level 11. Addressing and mitigating these challenges is crucial to facilitating women’s progression into leadership positions within the sector and is anticipated to contribute significantly to addressing the growing complexity of modern construction projects.