bluuebunny/crossref_metadata_2025 huggingface dataset:
Clonal structure and the specificity of vaccine-induced T cell response to SARS-CoV-2 Spike protein
Authors: Sheetikov Saveliy A. , Khmelevskaya Alexandra A. , Zornikova Ksenia V. , Zvyagin Ivan V. , Shomuradova Alina S. , Serdyuk Yana V. , Shakirova Naina T. , Peshkova Iuliia O. , Titov Aleksei , Romaniuk Dmitrii S. , Shagina Irina A. , Chudakov Dmitry M. , Kiryukhin Dmitry O. , Shcherbakova Olga V. , Khamaganova Ekaterina G. , Dzutseva Vitalina , Afanasiev Andrei , Bogolyubova Apollinariya V. , Efimov Grigory A.
Published: April 2024

Adenovirus vaccines, particularly the COVID-19 Ad5-nCoV adenovirus vaccine, have emerged as promising tools in the fight against infectious diseases. In this study, we investigated the structure of the T cell response to the Spike protein of the SARS-CoV-2 virus used in the COVID-19 Ad5-nCoV adenoviral vaccine in a phase 3 clinical trial (NCT04540419). In 69 participants, we collected peripheral blood samples at four time points after vaccination or placebo injection. Sequencing of T cell receptor repertoires from Spike-stimulated T cell cultures at day 14 from 17 vaccinated revealed a more diverse CD4+ T cell repertoire compared to CD8+. Nevertheless, CD8+ clonotypes accounted for more than half of the Spike-specific repertoire. Our longitudinal analysis showed a peak T cell response at day 14, followed by a decline until month 6. Remarkably, multiple T cell clonotypes persisted for at least 6 months after vaccination, as demonstrated by ex vivo stimulation. Examination of CDR3 regions revealed homologous sequences in both CD4+ and CD8+ clonotypes, with major CD8+ clonotypes sharing high similarity with annotated sequences specific for the NYNYLYRLF peptide, suggesting potential immunodominance. In conclusion, our study demonstrates the immunogenicity of the Ad5-nCoV adenoviral vaccine and highlights its ability to induce robust and durable T cell responses. These findings provide valuable insight into the efficacy of the vaccine against COVID-19 and provide critical information for ongoing efforts to control infectious diseases.
An undrained dynamic strain-pore pressure model for deep-water soft clays from the South China Sea
Authors: Jiao Houbin , Guo Xingsen , Fan Ning , Wu Hao , Nian Tingkai
Published: April 2024

With the increasing use of oceans for engineering purposes, such as the installation of suction anchors and pipelines, the stability of seabed structures has become a pivotal concern and is intricately linked to the characteristics of seabed soils. This study focuses specifically on deep-sea soft clay, a predominant seabed soil type distinguished by its high water content, thixotropy, and low permeability. These clays are vulnerable to destabilization and damage when disturbed, thereby posing threats to seabed installations. While the existing literature extensively examines the cyclic behavior of clay, considering factors such as the pore pressure response and strain and deformation characteristics, there is a notable gap in research addressing the behavior of deep-sea soft clay under comprehensive stress levels and prolonged cyclic loading. In this study, cyclic shear tests of the natural marine clay of the South China Sea were conducted, and the cyclic stress ratio (CSR), overpressure consolidation ratio (OCR), consolidation ratio (Kc), and loading frequency were varied. It was found that the CSR, OCR, and Kc significantly impact the cumulative dynamic strain in deep-sea soft clay during undrained cyclic dynamic tests. Higher CSR values lead to increased dynamic strain and structural failure risk. Subsequently, a dynamic strain-dynamic pore pressure development model was proposed. This model effectively captures the cumulative plastic deformation and dynamic pore pressure development, showing correlations with the CSR, OCR, and Kc, thus providing insights into the deformation and pore pressure trends in deep-sea clay under high cyclic dynamic loading conditions. This research not only furnishes essential background information but also addresses a critical gap in understanding the behavior of deep-sea soft clay under cyclic loading, thereby enhancing the safety and stability of seabed structures.
Cardiac autonomic dysfunction and structural remodeling: the potential mechanism to mediate the relationship between obstructive sleep apnea and cardiac arrhythmias
Authors: Chen Hao , Zhang Qingfeng , Hao Yueying , Zhang Jingyi , He Yang , Hu Ke
Published: April 2024

Background

Cardiac arrhythmias are very common in patients with obstructive sleep apnea (OSA), especially atrial fibrillation (AF) and nonsustained ventricular tachycardia (NVST). Cardiac autonomic dysfunction and structural remodeling caused by OSA provide the milieu for cardiac arrhythmia development. This study aimed to determine whether OSA is associated with various cardiac arrhythmias and investigate potential pathophysiologic pathways between them.

Methods

The analysis covered 600 patients with clinical suspicion of OSA hospitalized in Renmin Hospital of Wuhan University between January 2020 and May 2023. After undergoing sleep apnea monitor, all subjects received laboratory tests, Holter electrocardiography, and Echocardiography.

Results

Compared with those without OSA and adjusting for potential confounders, subjects with moderate OSA had three times the odds of AF (odds ratio [OR] 3.055; 95% confidence interval [CI], 1.002–9.316; p = 0.048). Subjects with severe OSA had three times the odds of AF (OR 3.881; 95% CI, 1.306–11.534; p = 0.015) and NSVT (OR 3.690; 95% CI, 0.809–16.036; p = 0.046). There were significant linear trends for the association between OSA severity with AF and NVST (p < 0.05). And this association was mediated by cardiac structural changes including left atrial diameter, left ventricular diastolic diameter, right atrial diameter and right ventricular diameter. In addition, the ratio of low-frequency and high-frequency individually mediated the association between severe OSA and NVST.

Conclusion

This study demonstrated that severe OSA was independently associated with AF and NSVT, and this association was mediated by autonomic nervous system changes and cardiac structural remodeling.
Declaration of Helsinki: ethical norm in pursuit of common global goals
Authors: Kurihara Chieko , Kerpel-Fronius Sandor , Becker Sander , Chan Anthony , Nagaty Yasmin , Naseem Shehla , Schenk Johanna , Matsuyama Kotone , Baroutsou Varvara
Published: April 2024

The World Medical Association’s Declaration of Helsinki is in the process of being revised. The following amendments are recommended to be incorporated in pursuit of the common goal of promoting health for all. 1. Data-driven research that facilitates broad informed consent and dynamic consent, assuring participant’s rights, and the sharing of individual participant data (IPD) and research results to promote open science and generate social value. 2. Risk minimisation in a placebo-controlled study and post-trial access to the best-proven interventions for all who need them. 3. A future-oriented research framework for co-creation with all the relevant stakeholders.
A red seaweed Kappaphycus alvarezii-based biostimulant (AgroGain®) improves the growth of Zea mays and impacts agricultural sustainability by beneficially priming rhizosphere soil microbial community
Authors: Nivetha Nagarajan , Shukla Pushp Sheel , Nori Sri Sailaja , Kumar Sawan , Suryanarayan Shrikumar
Published: April 2024

The overuse of chemical-based agricultural inputs has led to the degradation of soil with associated adverse effects on soil attributes and microbial population. This scenario leads to poor soil health and is reportedly on the rise globally. Additionally, chemical fertilizers pose serious risks to the ecosystem and human health. In this study, foliar sprays of biostimulant (AgroGain/LBS6) prepared from the cultivated, tropical red seaweed Kappaphycus alvarezii increased the phenotypic growth of Zea mays in terms of greater leaf area, total plant height, and shoot fresh and dry weights. In addition, LBS6 improved the accumulation of chlorophyll a and b, total carotenoids, total soluble sugars, amino acids, flavonoids, and phenolics in the treated plants. LBS6 applications also improved the total bacterial and fungal count in rhizospheric soil. The V3-V4 region of 16S rRNA gene from the soil metagenome was analyzed to study the abundance of bacterial communities which were increased in the rhizosphere of LBS6-treated plants. Treatments were found to enrich beneficial soil bacteria, i.e., Proteobacteria, especially the classes Alphaproteobacteria, Cyanobacteria, Firmicutes, Actinobacteriota, Verrucomicrobiota, Chloroflexi, and Acidobacteriota and several other phyla related to plant growth promotion. A metagenomic study of those soil samples from LBS6-sprayed plants was correlated with functional potential of soil microbiota. Enrichment of metabolisms such as nitrogen, sulfur, phosphorous, plant defense, amino acid, co-factors, and vitamins was observed in soils grown with LBS6-sprayed plants. These results were further confirmed by a significant increase in the activity of soil enzymes such as urease, acid phosphatase, FDAse, dehydrogenase, catalase, and biological index of fertility in the rhizosphere of LBS6-treated corn plant. These findings conclude that the foliar application of LBS6 on Z. mays improves and recruits beneficial microbes and alters soil ecology in a sustainable manner.
Effects of dietary energy levels on microorganisms and short-chain fatty acids of rumen and tight junction proteins in Honghe Yellow cattle
Authors: Yu Ye , Zi Yujie , Fu Runqi , Fu Binlong , Li Chenghuan , Lv Yaqi , Li Zhe , Wang Huayu , Leng Jing
Published: April 2024

This study was conducted to investigate the effects of dietary energy levels on microorganisms and short-chain fatty acids (SCFAs) of rumen and the expression of tight junction proteins in Honghe Yellow cattle. A total of fifteen male Honghe Yellow cattle were randomly divided into three treatments (five replicates per treatment), consisting of formulated energy concentrations of 5.90 MJ/kg (high-energy diet, group H), 5.60 MJ/kg (medium-energy diet, group M) and 5.30 MJ/kg (low-energy diet, group L). The results showed that compared with group H, the expression of Claudin-1 in rumen epithelium of groups M and L was increased, but the expression of ZO-1 was decreased (p < 0.05). Moreover, compared with group H, group M down-regulated the expression of Occludin and Claudin-1 in the brain (p < 0.05). For rumen bacteria, the dominant phyla included Bacteroidetes and Firmicutes, the abundance of Actinobacteriota in groups M and L was significantly increased compared with group H (p < 0.05). At the genus level, the relative abundance of Corynebacterium, Eubacterium_nodatum_group and Neisseraceae in groups M and L was significantly decreased compared with group H (p < 0.05). For rumen fungi, the dominant phyla included Basidiomycota, Ascomycota and Neocariastigomycota, the relative abundance of Ascomycetes was significantly higher than that of groups M and L compared with group H (p < 0.05). At the genus level, the relative abundance of Neocelimastigaceae and Myceliophthora in groups M and L was significantly reduced compared with group H (p < 0.05). Furthermore, the expression of Claudin-1 in rumen epithelium was significantly positively correlated with Actinobacteriota, Corynebacterium and Neisseriaceae. The expression of ZO-1 in the spinal cord was significantly positively correlated with Myceliophthora. The expression of Occludin in brain was positively correlated with valerate content (p < 0.05). In summary, dietary energy levels affected the rumen microbiota of Honghe Yellow cattle. The expression of Claudin-1 in rumen epithelium and the total SCFAs concentration were increased with decreasing dietary energy levels, but the expression of Claudin-1 in brain and ZO-1 in the spinal cord were reduced with decreasing dietary energy levels. Meanwhile, the rumen microbiota and SCFAs were significantly correlated with the expression of TJP.
Health benefits of bluefin tuna consumption: (Thunnus thynnus) as a case study
Authors: Chamorro F. , Cassani L. , Garcia-Oliveira P. , Barral-Martinez M. , Jorge A. O. S. , Pereira A. G. , Otero Paz , Fraga-Corral M. , P. P. Oliveira M. Beatriz , Prieto M. A.
Published: April 2024

Consumers are increasingly interested in food products with high nutritional value and health benefits. For instance, fish consumption is linked with diverse positive health benefits and the prevention of certain widespread disorders, such as obesity, metabolic syndrome, or cardiovascular diseases. These benefits have been attributed to its excellent nutritional value (large amounts of high-quality fatty acids, proteins, vitamins, and minerals) and bioactive compounds, while being relatively low-caloric. Atlantic bluefin tuna (Thunnus tynnus) is one of the most consumed species worldwide, motivated by its good nutritional and organoleptic characteristics. Recently, some organizations have proposed limitations on its consumption due to the presence of contaminants, mainly heavy metals such as mercury. However, several studies have reported that most specimens hold lower levels of contaminants than the established limits and that their richness in selenium effectively limits the contaminants’ bioaccessibility in the human body. Considering this situation, this study aims to provide baseline data about the nutritional composition and the latest evidence regarding the beneficial effects of Atlantic bluefin tuna consumption. A review of the risk-benefit ratio was also conducted to evaluate the safety of its consumption, considering the current suggested limitations to this species’ consumption.
Long-term surgical outcomes of bile duct tumor thrombus versus portal vein tumor thrombus for hepatocellular carcinoma: a propensity score matching analysis
Authors: Li Yi-Nan , Wei Shao-Ming , Fu Yang-Kai , Zeng Zhen-Xin , Huang Li-Ming , Lv Jia-Hui , Chen Wei-Zhao , Wei Yong-Gang , Zhang Zhi-Bo , Zhou Jian-Yin , Wu Jia-Yi , Yan Mao-Lin
Published: April 2024

Background

Portal vein tumor thrombus (PVTT) seriously affects the prognosis of hepatocellular carcinoma (HCC). However, whether bile duct tumor thrombus (BDTT) significantly affects the prognosis of HCC as much as PVTT remains unclear. We aimed to compare the long-term surgical outcomes of HCC with macroscopic PVTT (macro-PVTT) and macroscopic BDTT (macro-BDTT).

Methods

The data of HCC patients with macro-BDTT or macro-PVTT who underwent hemihepatectomy were retrospectively reviewed. A propensity score matching (PSM) analysis was performed to reduce the baseline imbalance. The recurrence-free survival (RFS) and overall survival (OS) rates were compared between the cohorts.

Results

Before PSM, the PVTT group had worse RFS and OS rates than the BDTT group (P = 0.043 and P = 0.008, respectively). Multivariate analyses identified PVTT (hazard ratio [HR] = 1.835, P = 0.016) and large HCC (HR = 1.553, P = 0.039) as independent risk factors for poor OS and RFS, respectively. After PSM, the PVTT group had worse RFS and OS rates than the BDTT group (P = 0.037 and P = 0.004, respectively). The 3- and 5-year OS rates were significantly higher in the BDTT group (59.5% and 52.1%, respectively) than in the PVTT group (33.3% and 20.2%, respectively).

Conclusion

Aggressive hemihepatectomy provides an acceptable prognosis for HCC patients with macro-BDTT. Furthermore, the long-term surgical outcomes of HCC patients with macro-BDTT were significantly better than those of HCC patients with macro-PVTT.
A new custom-made bivalve brace for pectus carinatum in children and adolescents: preliminary promising experience of 140 patients from a tertiary center
Authors: Frediani Simone , Zarfati Angelo , Pardi Valerio , Aloi Ivan , Bertocchini Arianna , Accinni Antonella , Beati Federico , Pasanisi Massimiliano , Inserra Alessandro
Published: April 2024

Introduction

International research suggests that poor patient compliance is the main cause of tutor failures in the context of potential novel orthopedic bivalve braces for conservative treatment of pectus carinatum. Our entire experimental study is based on the hypothesis that a rigid bivalve brace that patients can accept could solve the main problem associated with the conservative approach—poor compliance. The hypothesis was to reduce the thickness and weight of the classic bivalve brace to ensure concealment and make it sustainable enough to be worn several hours a day without compromising its therapeutic efficacy.

Materials and method

The research was conducted from January 2020 to December 2022 to ensure follow-up of all participants for at least 6 months. In 36 months, 140 patients with pectus carinatum were assessed and conservatively treated with the studied guardian to analyze the therapeutic efficacy of the bivalve brace and patient compliance. From the initial visit, the parents and patient were informed that this is a 2-year therapeutic course during which the bivalve brace should be worn at least 23 h a day (with 1 h of abstinence per day for routine personal hygiene practices). Compliance is the key to therapy success, and the duration of treatment depends on patient adherence.

Results

The exceptional effectiveness of the experimental brace was confirmed by both the questionnaire from the patients (with an average satisfaction rate of 8.9/10) and an assessment of the therapy's results by a properly selected medical committee (with a VAS scale satisfaction of 7.2/10 for symmetric forms and 7.1/10 for asymmetric ones).

Conclusion

In conclusion, the analyzed data confirmed the research hypotheses. First, none of the 140 patients had cardiovascular diseases directly related to their condition, confirming that pectus carinatum is a pathology of a purely aesthetic nature. Second, a cheap, lightweight, and easily obscured brace significantly improved patient compliance. Along with this, the social relevance of the aesthetic aspect today may be an important factor in motivating the study cohort to adhere to therapy. In the past, esthetics and appearance were less relevant at the social level, which may have contributed to the high abandonment and reduced compliance rates of the many studies in the literature.
Drug-drug interactions with oral anticoagulants: information consistency assessment of three commonly used online drug interactions databases in Switzerland
Authors: Coumau Claire , Gaspar Frederic , Terrier Jean , Schulthess-Lisibach Angela , Lutters Monika , Le Pogam Marie-Annick , Csajka Chantal
Published: April 2024

Background: Toxicity or treatment failure related to drug-drug interactions (DDIs) are known to significantly affect morbidity and hospitalization rates. Despite the availability of numerous databases for DDIs identification and management, their information often differs. Oral anticoagulants are deemed at risk of DDIs and a leading cause of adverse drug events, most of which being preventable. Although many databases include DDIs involving anticoagulants, none are specialized in them.

Aim and method: This study aims to compare the DDIs information content of four direct oral anticoagulants and two vitamin K antagonists in three major DDI databases used in Switzerland: Lexi-Interact, Pharmavista, and MediQ. It evaluates the consistency of DDIs information in terms of differences in severity rating systems, mechanism of interaction, extraction and documentation processes and transparency.

Results: This study revealed 2’496 DDIs for the six anticoagulants, with discrepant risk classifications. Only 13.2% of DDIs were common to all three databases. Overall concordance in risk classification (high, moderate, and low risk) was slight (Fleiss’ kappa = 0.131), while high-risk DDIs demonstrated a fair agreement (Fleiss’ kappa = 0.398). The nature and the mechanism of the DDIs were more consistent across databases. Qualitative assessments highlighted differences in the documentation process and transparency, and similarities for availability of risk classification and references.

Discussion: This study highlights the discrepancies between three commonly used DDI databases and the inconsistency in how terminology is standardised and incorporated when classifying these DDIs. It also highlights the need for the creation of specialised tools for anticoagulant-related interactions.
The involvement of soluble epoxide hydrolase in the development of cardiovascular diseases through epoxyeicosatrienoic acids
Authors: Jiang Shan , Han Siyi , Wang Dao Wen
Published: April 2024

Arachidonic acid (AA) has three main metabolic pathways: the cycloxygenases (COXs) pathway, the lipoxygenases (LOXs) pathway, and the cytochrome P450s (CYPs) pathway. AA produces epoxyeicosatrienoic acids (EETs) through the CYPs pathway. EETs are very unstable in vivo and can be degraded in seconds to minutes. EETs have multiple degradation pathways, but are mainly degraded in the presence of soluble epoxide hydrolase (sEH). sEH is an enzyme of bifunctional nature, and current research focuses on the activity of its C-terminal epoxide hydrolase (sEH-H), which hydrolyzes the EETs to the corresponding inactive or low activity diol. Previous studies have reported that EETs have cardiovascular protective effects, and the activity of sEH-H plays a role by degrading EETs and inhibiting their protective effects. The activity of sEH-H plays a different role in different cells, such as inhibiting endothelial cell proliferation and migration, but promoting vascular smooth muscle cell proliferation and migration. Therefore, it is of interest whether the activity of sEH-H is involved in the initiation and progression of cardiovascular diseases by affecting the function of different cells through EETs.
Cinnamaldehyde attenuates streptozocin-induced diabetic osteoporosis in a rat model by modulating netrin-1/DCC-UNC5B signal transduction
Authors: Ji Songjie , Zhao Bingjia , Gao Yuan , Xie Jun , Han Huijun , Wu Qunli , Yang Dan
Published: April 2024

Background: Cinnamaldehyde (CMD) is a major functional component of Cinnamomum verum and has shown treatment effects against diverse bone diseases. This study aimed to assess the anti-diabetic osteoporosis (DOP) potential of diabetes mellitus (DM) and to explore the underlying mechanism driving the activity of CMD.

Methods: A DOP model was induced via an intraperitoneal injection of streptozocin (STZ) into Sprague–Dawley rats, and then two different doses of CMD were administered to the rats. The effects of CMD on the strength, remodeling activity, and histological structure of the bones were assessed. Changes in the netrin-1 related pathways also were detected to elucidate the mechanism of the anti-DOP activity by CMD.

Results: CMD had no significant effect on the body weight or blood glucose level of the model rats. However, the data showed that CMD improved the bone strength and bone remodeling activity as well as attenuating the bone structure destruction in the DOP rats in a dose-dependent manner. The expression of netrin-1, DCC, UNC5B, RANKL, and OPG was suppressed, while the expression of TGF-β1, cathepsin K, TRAP, and RANK was induced by the STZ injection. CMD administration restored the expression of all of these indicators at both the mRNA and protein levels, indicating that the osteoclast activity was inhibited by CMD.

Conclusion: The current study demonstrated that CMD effectively attenuated bone impairments associated with DM in a STZ-induced DOP rat model, and the anti-DOP effects of CMD were associated with the modulation of netrin-1/DCC/UNC5B signal transduction.
Traditional Chinese medicine in osteoporosis: from pathogenesis to potential activity
Authors: Cao Gang , Hu ShaoQi , Ning Yan , Dou Xinyue , Ding Chuan , Wang Lu , Wang Zeping , Sang Xianan , Yang Qiao , Shi Jiangnan , Hao Min , Han Xin
Published: April 2024

Osteoporosis characterized by decreased bone density and mass, is a systemic bone disease with the destruction of microstructure and increase in fragility. Osteoporosis is attributed to multiple causes, including aging, inflammation, diabetes mellitus, and other factors induced by the adverse effects of medications. Without treatment, osteoporosis will further progress and bring great trouble to human life. Due to the various causes, the treatment of osteoporosis is mainly aimed at improving bone metabolism, inhibiting bone resorption, and promoting bone formation. Although the currently approved drugs can reduce the risk of fragility fractures in individuals, a single drug has limitations in terms of safety and effectiveness. By contrast, traditional Chinese medicine (TCM), a characteristic discipline in China, including syndrome differentiation, Chinese medicine prescription, and active ingredients, shows unique advantages in the treatment of osteoporosis and has received attention all over the world. Therefore, this review summarized the pathogenic factors, pathogenesis, therapy limitations, and advantages of TCM, aiming at providing new ideas for the prevention and treatment of OP.
Rhizobium symbiosis improves amino acid and secondary metabolite biosynthesis of tungsten-stressed soybean (Glycine max)
Authors: Preiner Julian , Steccari Irene , Oburger Eva , Wienkoop Stefanie
Published: April 2024

The industrially important transition metal tungsten (W) shares certain chemical properties with the essential plant micronutrient molybdenum and inhibits the activity of molybdoenzymes such as nitrate reductase, impacting plant growth. Furthermore, tungsten appears to interfere with metabolic processes on a much wider scale and to trigger common heavy metal stress response mechanisms. We have previously found evidence that the tungsten stress response of soybeans (Glycine max) grown with symbiotically associated N2-fixing rhizobia (Bradyrhizobium japonicum) differs from that observed in nitrogen-fertilized soy plants. This study aimed to investigate how association with symbiotic rhizobia affects the primary and secondary metabolite profiles of tungsten-stressed soybean and whether changes in metabolite composition enhance the plant’s resilience to tungsten. This comprehensive metabolomic and proteomic study presents further evidence that the tungsten-stress response of soybean plants is shaped by associated rhizobia. Symbiotically grown plants (N fix) were able to significantly increase the synthesis of an array of protective compounds such as phenols, polyamines, gluconic acid, and amino acids such as proline. This resulted in a higher antioxidant capacity, reduced root-to-shoot translocation of tungsten, and, potentially, also enhanced resilience of N fix plants compared to non-symbiotic counterparts (N fed). Taken together, our study revealed a symbiosis-specific metabolic readjustment in tungsten-stressed soybean plants and contributed to a deeper understanding of the mechanisms involved in the rhizobium-induced systemic resistance in response to heavy metals.
A new species, Aster yaoshanensis (Asteracae, Astereae), from Guangxi (China), based on morphology and molecular phylogenetic data
Authors: Zheng Xinyi , Qin Kun , Li Tingyu , Qu Tianmeng , Luo Junjia , Zhang Guojin , Li Bo , Li Pan , Fu Zhixi
Published: April 2024

Aster yaoshanensis sp. nov., a new species of the family Asteraceae is here described and illustrated. The species is presently known only from rock crevices of mountain valleys in Dayaoshan National Nature Reserve, Guangxi autonomous region, China. Phylogenetic analyses based on ITS sequences and complete plastome data have shown that this new species is a member of genus Aster with high support. Morphologically, it mostly resembles A. jishouensis, but it can be easily distinguished from the latter by bract indumentum (glabrous except margin ciliate vs. villous especially on veins abaxially, glabrous adaxially) and color (green vs. purple), shorter corolla (3.2–3.5 mm vs. 4.5–5.3 mm), bract stalk (obvious, ca.1.2 mm vs. sessile), and different distribution (Guangxi vs. Hunan). The detailed description, distribution map, and photos are provided. This study further elucidates the species identification, phylogeny and characteristic evolution of Aster.
Understanding South Korean women workers’ career transition experiences: using the career decision tree model
Authors: Kim Namhee , Kim Kyung Nam , Baek Pyounggu
Published: April 2024

Introduction

Relatively little research has explored non-Western women workers and their career transitions within their unique cultural contexts. Thus, more context-sensitive approaches to women’s career trajectories are needed.

Methods

Based on Bian and Wang’s Career Decision Tree Model (2019) as a conceptual framework, the reasons for South Korean women workers’ career transitions and influencing factors were explored using a qualitative approach with in-depth interviews with 35 South Korean women workers at various career stages.

Results and Discussion

Their main motive of career transitions was difficulty maintaining their physical and mental health, which stemmed from their demanding work life. A typical issue, the burden of child rearing and family responsibilities, was also reported, but it was not the primary reason for their career transitions. Instead, the women workers often mentioned these responsibilities along with other reasons. Other reasons were unresolved career interests and expectations associated with their lack of career goals and preparation prior to joining the labor market. These factors led to significant changes in women’s values and priorities along their career path, which finally triggered a decision to make a career transition. South Korean socio-cultural characteristics embedded in the South Korean women’s personal and organizational lives provide insights on how to interpret the findings. Although on the surface some of our findings appeared to confirm previous studies on women’s career transitions in Western-based literature, noteworthy differences were discovered when delving deeper into women’s career transitions in the South Korean context.
Child eating behavior predicts body mass index after 1 year: results from the Swiss Preschooler’s Health Study (SPLASHY)
Authors: Mihov Yoan , Meyer Andrea H. , Kakebeeke Tanja H. , Stülb Kerstin , Arhab Amar , Zysset Annina E. , Leeger-Aschmann Claudia S. , Schmutz Einat A. , Kriemler Susi , Jenni Oskar G. , Puder Jardena J. , Messerli-Bürgy Nadine , Munsch Simone
Published: April 2024

Child obesity is a growing global issue. Preventing early development of overweight and obesity requires identifying reliable risk factors for high body mass index (BMI) in children. Child eating behavior might be an important and malleable risk factor that can be reliably assessed with the parent-report Child Eating Behavior Questionnaire (CEBQ). Using a hierarchical dataset (children nested within child care centers) from a representative cohort of Swiss preschool children, we tested whether eating behavior, assessed with a 7-factor solution of the CEBQ, and BMI at baseline predicted the outcome BMI after 1 year, controlling for socioeconomic status (n = 555; 47% female; mean age = 3.9 years, range: 2.2–6.6; mean BMI = 16 kg/m2, range: 11.2–23; mean age- and sex-corrected z-transformed BMI, zBMI = 0.4, range −4 to +4.7). The statistical model explained 65.2% of zBMI at follow-up. Baseline zBMI was a strong positive predictor, uniquely explaining 48.8% of outcome variance. A linear combination of all CEBQ scales, taken together, explained 10.7% of outcome variance. Due to their intercorrelations, uniquely explained variance by any individual scale was of negligible clinical relevance. Only food responsiveness was a significant predictor, when accounting for all other predictors and covariates in the model, and uniquely explained only 0.4% of outcome variance. Altogether, our results confirm, extend, and refine previous research on eating behavior and zBMI in preschool children, by adjusting for covariates, accounting for intercorrelations between predictors, partitioning explained outcome variance, and providing standardized beta estimates. Our findings show the importance of carefully examining the contribution of predictors in multiple regression models for clinically relevant outcomes.
Mocking enactments: a case study of multimodal stance-stacking
Authors: de Vries Clarissa , Andries Fien , Meissl Katharina
Published: April 2024

Although research into multimodal stance-taking has gained momentum over the past years, the multimodal construction of so-called stacked stances has not yet received systematic attention in the literature. Mocking enactments are a prime example of such complex social actions as they are layered both interactionally and stance-related, and they rely significantly on the use of bodily visual resources, depicting rather than describing events and stances. Using Du Bois’ Stance Triangle as a framework, this study investigates mocking enactments as a case study to unravel the multimodal aspects of layered stance expressions. Drawing on three data sets—music instruction in Dutch, German, and English, spontaneous face-to-face interactions among friends in Dutch, and narrations on past events in Flemish Sign Language (VGT)—this study provides a qualitative exploration of mocking enactments across different communicative settings, languages, and modalities. The study achieves three main objectives: (1) illuminating how enactments are used for mocking, (2) identifying the layers of stance-taking at play, and (3) examining the multimodal construction of mocking enactments. Our analysis reveals various different uses of enactments for mocking. Aside from enacting the target of the mockery, participants can include other characters and viewpoints, highlighting the breadth of the phenomenon under scrutiny. Second, we uncover the layered construction of stance on all axes of the Stance Triangle (evaluation, positioning, and alignment). Third, we find that mocking enactments are embedded in highly evaluative contexts, indexed by the use of bodily visual resources. Interestingly, not all mocking enactments include a multimodally exaggerated depiction, but instead, some merely allude to an absurd hypothetical scenario. Our findings contribute to the growing body of literature on multimodal stance-taking, by showing how a nuanced interpretation of the Stance Triangle can offer a useful framework for analyzing layered stance acts.
Eco-guilt and eco-shame in everyday life: an exploratory study of the experiences, triggers, and reactions
Authors: Nielsen Rikke Sigmer , Gamborg Christian , Lund Thomas Bøker
Published: April 2024

Guilt and shame are often mentioned in the context of environmental problems. Exploring how such emotions affect individuals and their behavior is crucial to the effective promotion of more pro-environmental behavior and sustainable consumption. The aim of this article is to further the understanding of eco-guilt and eco-shame by studying these emotions among participants with differing levels of environmental concern (EC). Using a phenomenologically inspired approach, we conducted 18 in-depth interviews with Danish citizens. A clear connection between EC and the experienced emotions emerged, including how these emotions were triggered and how participants reacted to them. While individuals with high EC mainly experienced eco-guilt, individuals with low EC mainly experienced eco-shame and individuals with a medium level of EC experienced both emotions. Both eco-guilt and eco-shame can increase pro-environmental behaviors under certain conditions, but their effects are complex, and eco-shame in particular, risks leading to environmentally harmful behaviors. Therefore, harnessing these emotions to promote pro-environmental behavior introduces moral as well as practical considerations. The novelty of this study is that it questions the view that certain environmental emotions are inherently adaptive or maladaptive and underscores the importance of understanding the individual and social dynamics, which can affect how eco-guilt, eco-shame and their pro-environmental effects are experienced.
“It made me feel like a shit parent”: an intersectional analysis of pandemic mothering
Authors: Thorpe Holly , Barrett Nikki , Nemani Mihi Joy , O'Leary Grace , Ahmad Nida
Published: April 2024

The COVID-19 pandemic brought to the fore the everyday and exceptional challenges for mothers. Rarely, however, did research or social commentary acknowledge the multiplicities of motherhood during this prolonged period of risk, disruption, and uncertainty. This paper draws upon interviews with 24 mothers living in Aotearoa New Zealand during the pandemic, including women who were pregnant and gave birth during lockdowns, teenage mothers, single and low-income mothers, and working mothers. The sample was intentionally diverse, including Māori, Pacific, Asian and migrant mothers. Engaging an intersectional lens on motherhood and women's health, this paper builds upon and extends feminist research on mothers' experiences during the pandemic, highlighting the many different challenges facing mothers of diverse social, cultural, and economic positionalities and during various stages of motherhood. Across the sample, we reveal the significant emotional toll on mothers, particularly with the absence of critical social, medical and health support systems during lockdown periods and sustained social restrictions. Many of the women described how the pandemic affected their feelings about motherhood, prompting new reflections on their relationships with the home, family, work, and broader society. Despite some similarities, the pandemic experiences of Māori, Pacific, migrant and single mothers were further intensified by various forms of isolation, judgement, and discrimination. In this way, the pandemic shed light on the gendering of everyday maternal life, but also the need for more intersectional culturally and gender-responsive policies that acknowledge the multi-layered complexities of mothers' lives.
A combined methodological approach to characterize pig farming and its influence on the occurrence of interactions between wild boars and domestic pigs in Corsican micro-regions
Authors: Dupon Liane , Trabucco Bastien , Muñoz Facundo , Casabianca François , Charrier François , Laval Morgane , Jori Ferran
Published: April 2024

The pig sector in Corsica is based by a wide range of farming systems, mainly characterized on traditional extensive practices, which favor contacts between domestic and wild individuals. These contacts are suspected to influence the maintenance and the transmission of shared infectious diseases between both populations. Therefore, it is important to develop methods that allow to understand and anticipate their occurrence. Modeling these interactions requires accurate data on the presence, location and use of land on pig farms and farming practices, but such data are often unavailable, incomplete or outdated. In this study, we suggest a method to collect and analyze pig farming information that combines approaches from social sciences and epidemiology and enables a spatial representation of an index of potential interaction (IPI) between wild and domestic pigs at municipality level in the Corsican territory. As a first step of the process, interviews were conducted to gather information from 103 pig farms. Then, using hierarchical clustering, we identified five different clusters of pig farming practices which were evaluated and validated by local experts using participatory tools. The five pig farming clusters with their respective estimated levels of direct and indirect interactions with wild boars were combined in a linear equation with pig density to estimate a hypothetical index of potential interaction (IPI) in 155 municipalities. Our results revealed the diversity of pig farming practices across the island of Corsica and pointed out potential hotspots of interaction. Our method proved to be an effective way to collect and update information on the presence and typology of pig farms which has the potential to update official livestock production statistics. The spatial representation of an IPI between wild boars and domestic pigs in the Corsican territory could help design regional disease management strategies and policies to improve the control of certain shared pig pathogens in pig farms from Corsica.
Molecular characterization of porcine reproductive and respiratory syndrome virus identified in 2021 from Nepal
Authors: Prajapati Meera , Aryal Manita , Li Yanmin , Zhang Zhidong , Acharya Madhav Prasad , Clive Stephanie , Frossard Jean-Pierre
Published: April 2024

Porcine reproductive and respiratory syndrome (PRRS), an important viral disease of swine caused by PRRS virus (PRRSV) was first confirmed in Nepal in 2013. Since then, the virus has spread throughout the country and has now become endemic affecting the pig production nationally. However, molecular characterization of circulating strains has not been done in Nepal yet. In the present study, serum samples were collected from outbreak areas of different districts of Nepal and samples positive for PRRSV by ELISA were sent to Animal and Plant Health Agency (APHA), United Kingdom for sequence analysis. Out of 35 samples that were sent to APHA, only one sample was found positive by PCR and subjected to sequence analysis based on ORF5, ORF7 and Nsp2. The results from the phylogenetic analysis demonstrated that the PRRSV strain belongs to PRRSV-2 and lineage 8 strain. The sequences from the Nepalese PRRSV strain revealed a high degree of similarity with the strains isolated from India, China and Vietnam, with the closest genetic relatedness to the Indian isolates from 2020 and 2018. This is the first study on molecular characterization of PRRS virus circulating in Nepal. Further studies on strains circulating in Nepal are very essential to understand the virus diversity, its spread and evolution.
Classification performance of sEMG and kinematic parameters for distinguishing between non-lame and induced lameness conditions in horses
Authors: St. George Lindsay B. , Spoormakers Tijn J. P. , Hobbs Sarah Jane , Clayton Hilary M. , Roy Serge H. , Richards Jim , Serra Bragança Filipe M.
Published: April 2024

Despite its proven research applications, it remains unknown whether surface electromyography (sEMG) can be used clinically to discriminate non-lame from lame conditions in horses. This study compared the classification performance of sEMG absolute value (sEMGabs) and asymmetry (sEMGasym) parameters, alongside validated kinematic upper-body asymmetry parameters, for distinguishing non-lame from induced fore- (iFL) and hindlimb (iHL) lameness. Bilateral sEMG and 3D-kinematic data were collected from clinically non-lame horses (n = 8) during in-hand trot. iFL and iHL (2–3/5 AAEP) were induced on separate days using a modified horseshoe, with baseline data initially collected each day. sEMG signals were DC-offset removed, high-pass filtered (40 Hz), and full-wave rectified. Normalized, average rectified value (ARV) was calculated for each muscle and stride (sEMGabs), with the difference between right and left-side ARV representing sEMGasym. Asymmetry parameters (MinDiff, MaxDiff, Hip Hike) were calculated from poll, withers, and pelvis vertical displacement. Receiver-operating-characteristic (ROC) and area under the curve (AUC) analysis determined the accuracy of each parameter for distinguishing baseline from iFL or iHL. Both sEMG parameters performed better for detecting iHL (0.97 ≥ AUC ≥ 0.48) compared to iFL (0.77 ≥ AUC ≥ 0.49). sEMGabs performed better (0.97 ≥ AUC ≥ 0.49) than sEMGasym (0.76 ≥ AUC ≥ 0.48) for detecting both iFL and iHL. Like previous studies, MinDiff Poll and Pelvis asymmetry parameters (MinDiff, MaxDiff, Hip Hike) demonstrated excellent discrimination for iFL and iHL, respectively (AUC > 0.95). Findings support future development of multivariate lameness-detection approaches that combine kinematics and sEMG. This may provide a more comprehensive approach to diagnosis, treatment, and monitoring of equine lameness, by measuring the underlying functional cause(s) at a neuromuscular level.
Solar Irradiance Forecasting with Natural Language Processing of Cloud Observations and Interpretation of Results with Modified Shapley Additive Explanations
Authors: Matrenin Pavel V. , Gamaley Valeriy V. , Khalyasmaa Alexandra I. , Stepanova Alina I.
Published: April 2024

Forecasting the generation of solar power plants (SPPs) requires taking into account meteorological parameters that influence the difference between the solar irradiance at the top of the atmosphere calculated with high accuracy and the solar irradiance at the tilted plane of the solar panel on the Earth’s surface. One of the key factors is cloudiness, which can be presented not only as a percentage of the sky area covered by clouds but also many additional parameters, such as the type of clouds, the distribution of clouds across atmospheric layers, and their height. The use of machine learning algorithms to forecast the generation of solar power plants requires retrospective data over a long period and formalising the features; however, retrospective data with detailed information about cloudiness are normally recorded in the natural language format. This paper proposes an algorithm for processing such records to convert them into a binary feature vector. Experiments conducted on data from a real solar power plant showed that this algorithm increases the accuracy of short-term solar irradiance forecasts by 5–15%, depending on the quality metric used. At the same time, adding features makes the model less transparent to the user, which is a significant drawback from the point of view of explainable artificial intelligence. Therefore, the paper uses an additive explanation algorithm based on the Shapley vector to interpret the model’s output. It is shown that this approach allows the machine learning model to explain why it generates a particular forecast, which will provide a greater level of trust in intelligent information systems in the power industry.
Organizational and Individual Resources as Buffers of Work–Family Conflict Linkages to Affect: An Application of the Job Demands and Resources Health Impairment Model
Authors: Brandão Leonor , Matias Marisa
Published: April 2024

Work–family conflict is a phenomenon known to affect an individual’s well-being. However, its affective consequences are yet to be explored. In this study, we focus on understanding work–family conflict affective consequences on positive and negative affect. Our approach aims to refine the Job Demands and Resources model (JD-R model) by incorporating affect as a psychological mechanism in the health-impairment process and by exploring family-supportive organizational perceptions and psychological detachment as moderators. The final sample was composed of 195 couples, with men’s mean age around 46 years old (M = 46.85, SD = 0.34) and women’s age around 44 (M = 44.23, SD = 0.37). Men worked an average of 44.46 h per week (SD = 0.83), while women worked an average of 39.79 h per week (SD = 0.65). The majority of couples had full-time jobs (77.9% of men and 73.8% of women), worked fixed schedules (55.4% of men and 73.8% of women), were employed by others (75% of men and 82.8% of women), and worked for small companies (54.6% of men and 40% of women). Concerning education, most of the men (81.3%) and women (71.4%) attended high school or had less than 12 years of education. To test our moderation models, PROCESS version 4.1st macro for SPSS was used. Additional analyses included correlations and paired mean comparisons. Our findings indicate that work–family conflict correlates positively with negative affect and negatively with positive affect. Psychological detachment moderated the effect of work–family conflict on negative affect for women, but did not moderate the relationship with positive affect for men or women. Family-supportive organizational perceptions also did not moderate any of the proposed relationships. This study highlights how the ability to detach and separate family and professional domains is important and supports the health-impairment process of the Job Demands and Resources model through affective experiences.