bluuebunny/crossref_metadata_2025 huggingface dataset:
Bootstrapped Holt Method with Autoregressive Coefficients Based on Harmony Search Algorithm
Authors: Bas Eren , Egrioglu Erol , Yolcu Ufuk
Published: November 2021

Exponential smoothing methods are one of the classical time series forecasting methods. It is well known that exponential smoothing methods are powerful forecasting methods. In these methods, exponential smoothing parameters are fixed on time, and they should be estimated with efficient optimization algorithms. According to the time series component, a suitable exponential smoothing method should be preferred. The Holt method can produce successful forecasting results for time series that have a trend. In this study, the Holt method is modified by using time-varying smoothing parameters instead of fixed on time. Smoothing parameters are obtained for each observation from first-order autoregressive models. The parameters of the autoregressive models are estimated by using a harmony search algorithm, and the forecasts are obtained with a subsampling bootstrap approach. The main contribution of the paper is to consider the time-varying smoothing parameters with autoregressive equations and use the bootstrap method in an exponential smoothing method. The real-world time series are used to show the forecasting performance of the proposed method.
Kiwifruit (Actinidia spp.) Dietary Consumption for Constipation: A Systematic Review and Meta-Analysis
Authors: Antonelli Michele , Donelli Davide
Published: November 2021

The aim of this systematic review is to understand if kiwifruit dietary consumption can effectively improve constipation and intestinal function. PubMed, EMBASE, and Cochrane Library were systematically searched for relevant studies from inception up to September 2021. After database search, nine clinical studies were considered eligible for inclusion. Most trials were characterised by a limited number of study participants (median: 20, min: 11, max: 79) and had a cross-over design. On average, study participants ate from two to four kiwifruits a day for a period varying from three days to four weeks. Included trials almost exclusively involved young or middle-aged adults with a high female-to-male ratio, whereas direct evidence for elderly people (>65 years old) is scant. Moderate quality evidence indicated that kiwifruit dietary consumption can improve complete bowel movements per week and decrease stool consistency in both healthy subjects and patients with constipation due to irritable bowel syndrome, probably owing to the fruit fibre and water content. Kiwifruit dietary consumption can also have beneficial effects beyond intestinal motility, such as a mild anti-inflammatory and antioxidant effect on the gut barrier, due to a combined activity of all its nutrients (enzymes, vitamins, minerals). When only patients affected by constipation were considered, kiwifruit consumption was likely associated with a short-term significant increase in defecation frequency but not always with significant changes in stool consistency. These results were also supported by studies characterised by the highest methodological quality and confirmed by the meta-analysis about the effects of kiwifruit-based interventions on defecation frequency (g = 0.576; 95% CI: (0.174; 0.978); p = 0.012). Further investigations on the topic are recommended to strengthen the consistency of current evidence with larger trials.
Differences by School Location in Summer and School Monthly Weight Change: Findings from a Nationally Representative Sample
Authors: Hunt Ethan T. , Armstrong Bridget , Turner-McGrievy Brie M. , Beets Michael W. , Weaver Robert G.
Published: November 2021

Objectives: To examine changes in accelerations of Body Mass Index (BMI), age-and-sex specific body mass index (zBMI), and 95th percentile of BMI (%BMIp95) during the summer months and school year by school location designation (i.e., urban, suburban, exurban). This study utilized the Early Childhood Longitudinal Study Kindergarten Class of 2010–2011. Methods: Of the 18,174 children in the ECLS-K:2011 dataset, I restricted participants to those with at least two consecutive measures that occurred August/September or April/May. Mixed-effect regression analyses estimated differences in monthly change in BMI, zBMI, and %BMIp95 between the summer and school year while accounting for the ECLS-K complex sampling design. Models also examined differences in the magnitude of BMI, zBMI, and %BMIp95 change between the summer and school year by school location. Post-hoc Benjamini–Hochberg (BH) procedure set at 10% false discovery was incorporated to account for multiple comparisons. Results: A total of 1549 children (48% female, 42% White) had at least two consecutive measures that occurred in August/September or April/May. Among all locale classifications (i.e., urban, suburban, and exurban), children from high-income households comprised the largest proportions for each group (31%, 39%, and 37%), respectively. Among urban and suburban locations, Hispanic children comprised the largest proportions for both groups (43% and 44%), respectively. Among exurban locale classifications, White children comprised the largest proportion of children (60%). Children from suburban and exurban schools experienced significantly less accelerations in monthly zBMI gain when compared to their urban counterparts −0.038 (95CI = −0.071, −0.004) and −0.045 (95CI = −0.083, −0.007), respectively. Children from exurban schools experienced significantly less acceleration in monthly %BMIp95 during the summer months when compared to the school year −0.004 (95CI = −0.007, 0.000). Conclusions: This is one of the first studies to examine summer weight gain by school location. Summer appears to impact children more negatively from urban schools when compared to their suburban and exurban counterparts.
Nursing Students’ Subjective Happiness: A Social Network Analysis
Authors: Kim Eun-Joo , Lim Ji-Young , Kim Geun-Myun , Kim Seong-Kwang
Published: November 2021

Improving nursing students’ subjective happiness is germane for efficiency in the nursing profession. This study examined the subjective happiness of nursing students by applying social network analysis (SNA) and developing a strategy to improve the subjective happiness of nursing. The study adopted a cross sectional survey to measure subjective happiness and social network of 222 nursing students. The results revealed that the centralization index, which is a measure of intragroup interactions from the perspective of an entire network, was higher in the senior year compared with the junior year. Additionally, the indegree, outdegree, and centrality of the social network of students with a high level of subjective happiness were all found to be high. This result suggests that subjective happiness is not just an individual’s psychological perception, but can also be expressed more deeply depending on the subject’s social relationships. Based on the study’s results, to strengthen self-efficacy and resilience, it is necessary to utilize strategies that activate group dynamics, such as team activities, to improve subjective happiness. The findings can serve as basic data for future research focused on improving nursing students’ subjective happiness by consolidating team-learning social networks through a standardized program approach within a curriculum or extracurricular programs.
Educational Needs of School Nurses Regarding the Evidence-Based Management of Sickle Cell Disease
Authors: Shook Lisa M , Mosley Cami , Farrell Christina Bennett , Connelly Ann , Jones Cheryl L
Published: November 2021

Sickle cell disease (SCD) is a rare blood disorder that can have life-threatening complications. This presents a challenge for school nurses who may have had limited experience managing complications in the school setting. This study assessed the experience, self-reported knowledge, confidence and ability of school nurses in managing SCD in the school-setting and identified continuing educational needs and preferences. This study used a qualitative, descriptive approach. A survey was previously administered to over 400 school nurses who worked in K-12 schools in Ohio. Those participants who reported experience with managing SCD were invited to participate in a focus group or semi-structured interview. Data were interpreted using thematic analysis strategy. Four overarching themes emerged from the data: (1) perceived lack of support and resources, (2) self-reported lack of knowledge about SCD, (3) importance of partnerships with parents, and (4) need for continuing education and networking with other school nurses. Easily accessible, educational interventions, along with peer networking, can be designed to improve school nurse knowledge and confidence levels in managing SCD. These types of on-demand interventions are important as many school nurses reported infrequent exposure to students with SCD.
Assessing Ecological Vulnerability under Climate Change and Anthropogenic Influence in the Yangtze River Estuarine Island-Chongming Island, China
Authors: Peng Wanting , Wang Duoduo , Cai Yongli
Published: November 2021

Understanding and assessing ecological vulnerability for estuarine islands are important for maintaining estuarine island ecosystem services and its sustainable development. However, due to its complex fresh water–sea–land interaction mechanism and multiple stressors from both climate change and anthropogenic influence, a comprehensive evaluation of ecological vulnerability for estuarine islands has been limited. Therefore, taking the typical estuary island of Chongming Island as an example, we developed a comprehensive evaluation system of ecological vulnerability for an estuarine island ecosystem based on the pressure-state-response (PSR) conceptual model, and explored the spatial and temporal distribution of ecological vulnerability in 2005 and 2015. The results indicated that the main pressures of Chongming Island from saltwater intrusion intensity and land use intensity were mainly distributed in northern coastal areas and eastern areas of wetland; the ecological vulnerability index (EV) of Chongming Island showed a slight decrease from 2005 to 2015; and three categories of towns based on ecological vulnerability assessment for an eco-island planning and environmental management were identified. Our study provides an effective evaluation system of ecological vulnerability for estuarine islands, which could be helpful for planners and decision makers in improving eco-island planning and environmental management.
Relationships between Molecular Structure of Carbohydrates and Their Dynamic Hydration Shells Revealed by Terahertz Time-Domain Spectroscopy
Authors: Penkov Nikita V.
Published: November 2021

Despite more than a century of research on the hydration of biomolecules, the hydration of carbohydrates is insufficiently studied. An approach to studying dynamic hydration shells of carbohydrates in aqueous solutions based on terahertz time-domain spectroscopy assay is developed in the current work. Monosaccharides (glucose, galactose, galacturonic acid) and polysaccharides (dextran, amylopectin, polygalacturonic acid) solutions were studied. The contribution of the dissolved carbohydrates was subtracted from the measured dielectric permittivities of aqueous solutions based on the corresponding effective medium models. The obtained dielectric permittivities of the water phase were used to calculate the parameters describing intermolecular relaxation and oscillatory processes in water. It is established that all of the analyzed carbohydrates lead to the increase of the binding degree of water. Hydration shells of monosaccharides are characterized by elevated numbers of hydrogen bonds and their mean energies compared to undisturbed water, as well as by elevated numbers and the lifetime of free water molecules. The axial orientation of the OH(4) group of sugar facilitates a wider distribution of hydrogen bond energies in hydration shells compared to equatorial orientation. The presence of the carboxylic group affects water structure significantly. The hydration of polysaccharides is less apparent than that of monosaccharides, and it depends on the type of glycosidic bonds.
Effect of Central Administration of Brain-Derived Neurotrophic Factor (BDNF) on Behavior and Brain Monoamine Metabolism in New Recombinant Mouse Lines Differing by 5-HT1A Receptor Functionality
Authors: Bazovkina Darya , Naumenko Vladimir , Bazhenova Ekaterina , Kondaurova Elena
Published: November 2021

Experiments were carried out on recombinant B6.CBA-D13Mit76C (B6-M76C) and B6.CBA-D13Mit76B (B6-M76B) mouse lines created by transferring a 102.73–118.83 Mbp fragment of chromosome 13, containing the 5-HT1A receptor gene, from CBA or C57BL/6 strains to a C57BL/6 genetic background, correspondingly. We have recently shown different levels of 5-HT1A receptor functionality in these mouse lines. The administration of BDNF (300 ng/mouse, i.c.v.) increased the levels of exploratory activity and intermale aggression only in B6-M76B mice, without affecting depressive-like behavior in both lines. In B6-M76B mice the behavioral alterations were accompanied by a decrease in the 5-HT2A receptor functional activity and the augmentation of levels of serotonin and its main metabolite, 5-HIAA (5-hydroxyindoleacetic acid), in the midbrain. Moreover, the levels of dopamine and its main metabolites, HVA (homovanillic acid) and DOPAC (3,4-dihydroxyphenylacetic acid), were also elevated in the striatum of B6-M76B mice after BDNF treatment. In B6-M76C mice, central BDNF administration led only to a reduction in the functional activity of the 5-HT1A receptor and a rise in DOPAC levels in the midbrain. The obtained data suggest the importance of the 102.73–118.83 Mbp fragment of mouse chromosome 13, which contains the 5-HT1A receptor gene, for BDNF-induced alterations in behavior and the brain monoamine system.
Vulnerability Assessment of Maize Yield Affected by Precipitation Fluctuations: A Northeastern United States Case Study
Authors: Su Peng , Li Shiqi , Wang Jing’ai , Liu Fenggui
Published: November 2021

Crop yields are threatened by global climate change. Maize has high water requirements, and precipitation fluctuations can impact its yield. In this study, we used the Environmental Policy Integrated Climate (EPIC) model to simulate maize yields in eight northeastern U.S. states. We used precipitation fluctuations and the coefficient of variation (CV) of yield as indicators to construct a vulnerability curve for the CV of yield and precipitation fluctuations. We then evaluated the vulnerability of maize yields under precipitation fluctuations in the region. We obtained the following results: (1) the fitted vulnerability curves were classified into three categories (positive slope, negative slope, and insignificant fit), of which the first category accounted for about 92.7%, indicating that the CV of maize yield was positively correlated with precipitation fluctuations in most parts of the study area; and (2) the CV of maize yield under 11 precipitation fluctuation scenarios was mapped to express the CV at the spatial level, and the maize yield in Connecticut and Maryland proved to be the most sensitive to precipitation fluctuations. This study provided a theoretical and experimental basis for the prevention of maize yield risk under fluctuating precipitation conditions.
Improved Non-Piezoelectric Electric Properties Based on La Modulated Ferroelectric-Ergodic Relaxor Transition in (Bi0.5Na0.5)TiO3-Ba(Ti, Zr)O3 Ceramics
Authors: Zhang Xingru , Xiao Yinan , Du Beining , Li Yueming , Wu Yuandong , Sheng Liyuan , Tan Wenchang
Published: November 2021

The characteristic transition from ferroelectric (FE) to ergodic relaxor (ER) state in (Bi0.5Na0.5)TiO3 (BNT) based lead-free ceramics provides an efficient approach to bring a highly ordered phase back to a disordered one. It would be rational to utilize this transition to improve relevant non-piezoelectric properties based on domain decomposition. In this work, different La contents were introduced to 0.93(Bi0.5Na0.5)TiO3-0.07Ba(Ti0.945Zr0.055)O3 ceramics (BNT-BZT-xLa) to induce evolution of ergodic degree. The results reveal that with increasing La content, both the FE-ER transition temperature TF-R and depolarization temperature Td shift towards room temperature, implying a deepened ergodic degree. By modulation of ergodic degree, thermally stimulated depolarization current experiment shows a higher current density peak, and corresponding pyroelectric coefficient increases from 2.46 to 2.81 μC/(cm2∙°C) at Td. For refrigeration, the indirect measurement demonstrates the ΔT maximum increases from 1.1 K to 1.4 K, indicating an enhanced electrocaloric effect. Moreover, the optimized energy storage effect is observed after La doping. With appearance of “slimmer” P-E loops, both calculated recoverable energy storage density Wrec and storage efficiency η increase to 0.23 J/cm3 and 22.8%, respectively. These results denote La doping conduces to the improvement of non-piezoelectric properties of BNT-based ceramics in a certain range. Therefore, La doping should be an adopted modification strategy for lead-free ceramics used in areas like refrigerator and pulse capacitors.
Efficacy of Reciprocating Instruments in the Removal of Bioceramic and Epoxy Resin-Based Sealers: Micro-CT Analysis
Authors: Rajda Marko , Miletić Ivana , Baršić Gorana , Krmek Silvana Jukić , Šnjarić Damir , Baraba Anja
Published: November 2021

The objective of this study was to evaluate the efficacy of reciprocating instruments in the removal of bioceramic and epoxy resin-based sealers using micro-CT analysis. Root canals of 40 extracted human teeth were instrumented with a size R25 Reciproc instrument. Specimens were randomly divided into two groups (n = 20) according to the root canal filling material. In the first group, root canals were obturated with AH Plus sealer and Reciproc R25 gutta-percha. In the second group, a combination of bioceramic gutta-percha (TotalFill BC) and bioceramic sealer (TotalFill BC) was used. After one week, the retreatment of all specimens was performed using Reciproc instruments. To analyze the differences in the filling remnants, specimens were scanned in a micro-CT device after obturation and after the retreatment procedure. Statistical analysis was performed using the Mann–Whitney U test (p < 0.05). A combination of bioceramic sealer and bioceramic gutta-percha was more effectively removed from canals using a reciprocating instrument, with a filling remnants volume of 4.01 ± 3.13 mm3, in comparison to the combination of epoxy resin-based sealer and gutta-percha (6.96 ± 2.70 mm3) (p < 0.05). A reciprocating instrument was more effective in removing bioceramic sealers than epoxy resin-based sealers, although none of the root canal filling materials were completely removed from the root canals.
Faience Waste for the Production of Wall Products
Authors: Petropavlovskii Kirill , Novichenkova Tatiana , Petropavlovskaya Victoria , Sulman Mikhail , Fediuk Roman , Amran Mugahed
Published: November 2021

Increasing the efficiency of using gypsum binders can be carried out by using not natural gypsum raw materials, but calcium sulfate-containing waste from various industries (phosphogypsum, borogypsum, citrogypsum, etc.). As the main source material in the work, we used gypsum-containing waste from a faience factory in the form of waste molds for casting dishes, souvenirs and plumbing fixtures. It has been established that the optimal binding system is formed by mixing powders of dihydrate technogenic gypsum from a coarse and fine earthenware factory with average particle diameters of 3.473 microns and 3.065 microns in a percentage ratio of 30:70, respectively. Using a computer software developed by the authors, which makes it possible to simulate the microstructure of a raw mixture taking into account the contact interaction of particles and calculate the average coordination number, models of binary packing of particles were constructed at various ratios of their diameters. Studies of the strength of composites obtained on the basis of bidisperse systems have shown the presence of an extremum in the region of mixtures containing 30% coarse powder. With optimal packing, a large number of phase contacts are formed due to the regulation of the grain composition of the bidisperse system. It was revealed that a brick based on the waste of two-water gypsum from earthenware production has 2.5–5 times better characteristics of compressive strength than traditional building wall products based on natural gypsum. At the same time, the strength immediately after molding is more than 3 times higher than that of traditional gypsum products. Even higher indicators are achieved when adding microcalcite in addition to the waste of earthenware production, in this case, the compressive strength is 3–6 times higher, and the strength immediately after molding is almost 3 times higher than that of traditional gypsum products.
Pharmacological Treatment for Acute Traumatic Musculoskeletal Pain in Athletes
Authors: de Sire Alessandro , Marotta Nicola , Lippi Lorenzo , Scaturro Dalila , Farì Giacomo , Liccardi Alfonso , Moggio Lucrezia , Letizia Mauro Giulia , Ammendolia Antonio , Invernizzi Marco
Published: November 2021

Pain management is a crucial issue for athletes who train and compete at the highest performance levels. There are still evidence gaps for the use of analgesics for sports injuries despite the growing interest in training and competition settings. However, high-quality research is needed to determine the most appropriate and optimal timing and formulations in non-steroidal anti-inflammatory drug and opioid management, particularly given the strictness of anti-doping regulations. Indeed, the role of pharmacological therapy in reducing acute traumatic pain in athletes should still be addressed to minimize the timing of return to sport. Therefore, the aim of this comprehensive review was to summarize the current evidence about pain management in the setting of acute injury in elite athletes, providing the most informed strategy for pain relief and performance recovery.
Reaching the Challenging Diagnosis of Complicated Liver Hydatid Disease: A Single Institution’s Experience from an Endemic Area
Authors: Christodoulidis Gregory , Samara Athina A. , Diamantis Alexandros , Floros Theodoros , Sgantzou Ioanna-Konstantina , Karakantas Kostas-Sotirios , Zotos Prokopis-Andreas , Koutras Antonios , Janho Michel B. , Tepetes Konstantinos
Published: November 2021

Background and Objectives: Hydatid disease (HD) remains a significant public health issue causing morbidity and mortality in many Mediterranean countries. Material and Methods: The present cohort study included 50 consecutive patients with liver hydatid disease who underwent surgery in a tertiary University Hospital. A total of 18 patients (36%) had a case of complicated HD, including simple communication of the cyst with the biliary tree (6 cases), rupture of the cyst into the biliary tree (6 cases), presence of a bronco-biliary fistula (2 cases), rupture of the cyst in the peritoneal cavity (2 cases), and rupture of the cyst and formation of a hepatic abscess (2 cases). Endoscopic retrograde cholangiopancreatography (ERCP) was pre-operatively performed on six patients. Results: The main clinical symptom presented was right upper quadrant pain in 16 patients (88%), which was associated with high fever (>39 °C) in 14 patients (78%). C-reactive protein (CRP) was the primary indicator of a complicated HD (p = 0.003); however, it was only elevated in 67% of cases. CRP was a more sensitive indicator of a rupture in the biliary tree cyst (p = 0.02). Computer tomography (CT) detected more cases (44%) of a complicated HD than ultrasonography (US) (25%); however, the difference was not statistically significant. Conclusions: For prevention and control of HD, a high suspicion of the disease leading to early referral to specialized centers, mainly in endemic areas, is required. Prior to surgical or percutaneous intervention, a combination of imaging and laboratory findings are essential in diagnosing a complicated case and avoiding unnecessary interventions.
Structural Diversity of Ubiquitin E3 Ligase
Authors: Toma-Fukai Sachiko , Shimizu Toshiyuki
Published: November 2021

The post-translational modification of proteins regulates many biological processes. Their dysfunction relates to diseases. Ubiquitination is one of the post-translational modifications that target lysine residue and regulate many cellular processes. Three enzymes are required for achieving the ubiquitination reaction: ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), and ubiquitin ligase (E3). E3s play a pivotal role in selecting substrates. Many structural studies have been conducted to reveal the molecular mechanism of the ubiquitination reaction. Recently, the structure of PCAF_N, a newly categorized E3 ligase, was reported. We present a review of the recent progress toward the structural understanding of E3 ligases.
How Next-Generation Sequencing Is Changing Clinical Neurogenetics
Authors: Orsucci Daniele
Published: November 2021

Until recently, most general neurologists were not interested in neurogenetics [...]
Alcohol and the Brain
Authors: Nutt David , Hayes Alexandra , Fonville Leon , Zafar Rayyan , Palmer Emily O.C. , Paterson Louise , Lingford-Hughes Anne
Published: November 2021

Alcohol works on the brain to produce its desired effects, e.g., sociability and intoxication, and hence the brain is an important organ for exploring subsequent harms. These come in many different forms such as the consequences of damage during intoxication, e.g., from falls and fights, damage from withdrawal, damage from the toxicity of alcohol and its metabolites and altered brain structure and function with implications for behavioral processes such as craving and addiction. On top of that are peripheral factors that compound brain damage such as poor diet, vitamin deficiencies leading to Wernicke-Korsakoff syndrome. Prenatal alcohol exposure can also have a profound impact on brain development and lead to irremediable changes of fetal alcohol syndrome. This chapter briefly reviews aspects of these with a particular focus on recent brain imaging results. Cardiovascular effects of alcohol that lead to brain pathology are not covered as they are dealt with elsewhere in the volume.
Influence of Nutrition, Food and Diet-Related Interventions in the Workplace: A Meta-Analysis with Meta-Regression
Authors: Melián-Fleitas Liliana , Franco-Pérez Álvaro , Caballero Pablo , Sanz-Lorente María , Wanden-Berghe Carmina , Sanz-Valero Javier
Published: November 2021

Objective: To review the scientific literature on the influence of verified nutrition, food and diet interventions on occupational health. Method: This study involved a critical analysis of articles retrieved from MEDLINE (via PubMed), Embase, Cochrane Library, PsycINFO, Scopus, Web of Science, Latin American and Caribbean Health Sciences Literature (LILACS) and Medicina en Español (MEDES) using the descriptors “Diet, Food, and Nutrition” and “Occupational Health” and applying the filters “Clinical Trial”, “Humans” and “Adult: 19+ years”; the search was conducted on 29 May 2021. Results: A total of 401 references were retrieved from the bibliographic databases, with an additional 16 identified through a secondary search; among the studies retrieved, 34 clinical trials were selected after applying the inclusion and exclusion criteria. The interventions were grouped into seven categories: (1) dietary interventions associated with exercise or educational programs; (2) individual environmental interventions or other educational actions; (3) educational interventions oriented toward lifestyle, dietetics, physical activity and stress management; (4) economic incentives; (5) multicomponent interventions (combination of mindfulness, e-coaching and the addition of fruits and vegetables); or dietary interventions (facilitating greater food supply in cafeterias); or interventions focused on physical exercise. Conclusions: Given that most people spend a large part of their time in the workplace and, therefore, eat at least one of their daily meals there, well-planned interventions—preferably including several strategies—have been demonstrated, in general, as useful for combating overweight and obesity. From the meta-regression study, it was observed that the interventions give better results in people who presented high Body Mass Index (BMI) values (obesity). In contrast, intervention 2 (interventions related to workplace environment) would not give the expected results (it would increase the BMI).
Effects of Early Weight Gain Velocity, Diet Quality, and Snack Food Access on Toddler Weight Status at 1.5 Years: Follow-Up of a Randomized Controlled Infant Formula Trial
Authors: Mennella Julie A. , Smethers Alissa D. , Decker Jessica E. , Delahanty Michelle T. , Stallings Virginia A. , Trabulsi Jillian C.
Published: November 2021

This study followed children who participated in a feeding trial in which the type of randomized infant formula fed from 2 weeks significantly affected weight gain velocity during the first 4 months and weight-for-length Z (WLZ) scores up to 11.5 months. We focused on measures of anthropometry, dietary intakes, and parenting related to the provision of snack foods that were collected at the end of the trial (1 year) and the 1.5 years follow-up visit. We not only describe what toddlers are eating, but we also determined the independent and/or interactive effects of randomized formula group, early weight gain velocity, the nutrient content of the post-formula diet, and maternal snack food practices, on toddlers’ weight status. Diet quality underwent drastic changes during this 6-month period. As infant formula disappeared from the diet, fruit and 100% fruit juice intake increased slightly, while intake of “What We Eat in America” food categories sweetened beverages and snacks and sweets more than doubled. Added sugars accounted for 5% of energy needs at 1 year and 9% at 1.5 years. Generalized linear mixed models revealed that, independent of the randomized formula group, greater velocities of weight gain during early infancy and lower access to snacks as toddlers predicted higher WLZ and a greater proportion of toddlers with overweight at 1.5 years. Energy and added sugar intake had no significant effects. These findings add to the growing body of evidence that unhealthy dietary habits are formed even before formula weaning and that, along with improving early diet, transient rapid weight gain and parental feeding practices are modifiable determinants that may reduce risks for obesity.
Fasting Interventions for Stress, Anxiety and Depressive Symptoms: A Systematic Review and Meta-Analysis
Authors: Berthelot Elisa , Etchecopar-Etchart Damien , Thellier Dimitri , Lancon Christophe , Boyer Laurent , Fond Guillaume
Published: November 2021

Background. Fasting interventions have shown effectiveness in alleviating stress, anxiety and depressive symptoms. However, no quantitative analysis has been carried out thus far. The objective was to determine the effectiveness of fasting interventions on stress, anxiety and depression and if these interventions were associated with increased or decreased fatigue/energy. Methods. Overall, 11 studies and 1436 participants were included in the quantitative analyses. Results. After limiting analyses to randomized controlled trials with low risk of bias, we found that fasting groups had lower anxiety (b = −0.508, p = 0.038), depression levels (b= −0.281, p = 0.012) and body mass index compared to controls without increased fatigue. There was no publication bias and no heterogeneity for these results. These interventions were safe, even in patients with type 2 diabetes. Conclusions. These results should be taken with a caveat. These results are preliminary and encouraging and fasting appears to be a safe intervention. Data are not sufficient to recommend one fasting intervention more than the others. No study was carried out in psychiatric populations and further trials should be carried out in these populations that may be good candidates for fasting interventions.
Molecular Detection of Echinococcus granulosus Sensu Stricto in Environmental Dog Faecal Samples from the Magallanes Region, Patagonia, Chile
Authors: Alvarez Juan Francisco , Ruiz Raúl , Ríos Juan , Alvarez Rojas Cristian A.
Published: November 2021

Echinococcus granulosus is endemic in the Magallanes region and other areas of Chile. After a successful control programme implemented between 1979 and 2004, dogs’ prevalence decreased from 70% to 0.5%. Since the end of the programme, no prevalence study of canine echinococcosis has been performed in this region. Dog faecal samples were collected from epidemiological units for DNA isolation and polymerase chain reaction (PCR) to detect E. granulosus. In addition, dog owners were required to answer a questionnaire for a risk factor analysis. We collected 1069 environmental dog faecal samples from 267 urban, 241 periurban, and 61 rural epidemiological units. E. granulosus was found in 11/61 (18%) and 1/241 (0.4%) epidemiological units from rural and periurban areas, respectively. The Ultima Esperanza province showed the highest prevalence with eight out of 16 units with faeces positive to E. granulosus showing a main spatial cluster of canine echinococcosis. None of the risk factors investigated showed a statistical significance with positive units. This study shows the active transmission of the parasite in the Magallanes region, especially in the Ultima Esperanza province, with a possible re-emergence of the parasite. Further studies focusing on the incidence in humans are required together with the reestablishment of the compulsory deworming of dogs.
The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a Streptococcus mutans Cultivated Environment
Authors: Kung Jung-Chang , Wang Wei-Hsun , Chiang Yu-Ching , Yang-Wang Yuan-Ting , Wang Yueh-Ching , Chen Wen-Cheng , Shih Chi-Jen
Published: November 2021

The aim of this study was to evaluate the remineralization and antibacterial effect of silver-containing mesoporous bioactive glass (MBG-Ag) sealing combined with Er:YAG laser irradiation on human demineralized dentin specimens in a Streptococcus mutans cultivated environment. A total of 48 human dentin specimens were randomly divided into four groups. The characteristics of MBG-Ag and the occlusion efficiency of the dentinal tubules were analyzed using X-ray diffraction patterns, Fourier-transform infrared spectroscopy, scanning electron microscope images and energy dispersive X-ray spectroscopy. Moreover, the antibacterial activity against Streptococcus mutans was evaluated by colony formation assay. The results showed that the dentin specimens with Er:YAG laser irradiation can form a melted occlusion with a size of 3–4 µm. MBG-Ag promoted the deposition of numerous crystal particles on the dentinal surface, reaching the deepest penetration depth of 70 μm. The results suggested that both MBG-Ag and laser have the ability to enhance the remineralization and precipitation of hydroxyapatite crystals. While the results showed that MBG-Ag sealing combined with the thermomechanical subablation mode of Er:YAG laser irradiation-induced dense crystalline deposition, reaching a penetration depth of more than 300 µm, silver nanoparticles without good absorption of the Er:YAG laser resulted in a heterogeneous radiated surface. Er:YAG laser irradiation with a low energy and pulse rate cannot completely inhibit the growth of S. mutans, but MBG-Ag sealing reached the bactericidal concentration. It was concluded that the simultaneous application of MBG-Ag sealing and Er:YAG laser treatment can prevent the drawbacks of their independent uses, resulting in a superior form of treatment for dentin hypersensitivity.
Advances on Greener Asymmetric Synthesis of Antiviral Drugs via Organocatalysis
Authors: da Silva Everton , Vidal Hérika , Corrêa Arlene
Published: November 2021

Viral infections cause many severe human diseases, being responsible for remarkably high mortality rates. In this sense, both the academy and the pharmaceutical industry are continuously searching for new compounds with antiviral activity, and in addition, face the challenge of developing greener and more efficient methods to synthesize these compounds. This becomes even more important with drugs possessing stereogenic centers as highly enantioselective processes are required. In this minireview, the advances achieved to improve synthetic routes efficiency and sustainability of important commercially antiviral chiral drugs are discussed, highlighting the use of organocatalytic methods.
Cationic and Biocompatible Polymer/Lipid Nanoparticles as Immunoadjuvants
Authors: Pérez-Betancourt Yunys , Araujo Péricles Marques , Távora Bianca de Carvalho Lins Fernandes , Pereira Daniele Rodrigues , Faquim-Mauro Eliana Lima , Carmona-Ribeiro Ana Maria
Published: November 2021

Nanostructures have been of paramount importance for developing immunoadjuvants. They must be cationic and non-cytotoxic, easily assembling with usually oppositely charged antigens such as proteins, haptens or nucleic acids for use in vaccines. We obtained optimal hybrid nanoparticles (NPs) from the biocompatible polymer poly(methyl methacrylate) (PMMA) and the cationic lipid dioctadecyl dimethyl ammonium bromide (DODAB) by emulsion polymerization of methyl methacrylate (MMA) in the presence of DODAB. NPs adsorbed ovalbumin (OVA) as a model antigen and we determined their adjuvant properties. Interestingly, they elicited high double immune responses of the cellular and humoral types overcoming the poor biocompatibility of DODAB-based adjuvants of the bilayer type. The results suggested that the novel adjuvant would be possibly of use in a variety of vaccines.
Population Pharmacokinetics of Meropenem in Critically Ill Korean Patients and Effects of Extracorporeal Membrane Oxygenation
Authors: Lee Dong-Hwan , Kim Hyoung-Soo , Park Sunghoon , Kim Hwan-il , Lee Sun-Hee , Kim Yong-Kyun
Published: November 2021

Limited studies have investigated population pharmacokinetic (PK) models and optimal dosage regimens of meropenem for critically ill adult patients using the probability of target attainment, including patients receiving extracorporeal membrane oxygenation (ECMO). A population PK analysis was conducted using non-linear mixed-effect modeling. Monte Carlo simulation was used to determine for how long the free drug concentration was above the minimum inhibitory concentration (MIC) at steady state conditions in patients with various degrees of renal function. Meropenem PK in critically ill patients was described using a two-compartment model, in which glomerular filtration rate was identified as a covariate for clearance. ECMO did not affect meropenem PK. The simulation results showed that the current meropenem dosing regimen would be sufficient for attaining 40%fT>MIC for Pseudomonas aeruginosa at MIC ≤ 4 mg/L. Prolonged infusion over 3 h or a high-dosage regimen of 2 g/8 h was needed for MIC > 2 mg/L or in patients with augmented renal clearance, for a target of 100%fT>MIC or 100%fT>4XMIC. Our study suggests that clinicians should consider prolonged infusion or a high-dosage regimen of meropenem, particularly when treating critically ill patients with augmented renal clearance or those infected with pathogens with decreased in vitro susceptibility, regardless of ECMO support.
Compartmental and COMSOL Multiphysics 3D Modeling of Drug Diffusion to the Vitreous Following the Administration of a Sustained-Release Drug Delivery System
Authors: Dosmar Emily , Vuotto Gabrielle , Su Xingqi , Roberts Emily , Lannoy Abigail , Bailey Garet J. , Mieler William F. , Kang-Mieler Jennifer J.
Published: November 2021

The purpose of this study was to examine antibiotic drug transport from a hydrogel drug delivery system (DDS) using a computational model and a 3D model of the eye. Hydrogel DDSs loaded with vancomycin (VAN) were synthesized and release behavior was characterized in vitro. Four different compartmental and four COMSOL models of the eye were developed to describe transport into the vitreous originating from a DDS placed topically, in the subconjunctiva, subretinally, and intravitreally. The concentration of the simulated DDS was assumed to be the initial concentration of the hydrogel DDS. The simulation was executed over 1500 and 100 h for the compartmental and COMSOL models, respectively. Based on the MATLAB model, topical, subconjunctival, subretinal and vitreous administration took most (~500 h to least (0 h) amount of time to reach peak concentrations in the vitreous, respectively. All routes successfully achieved therapeutic levels of drug (0.007 mg/mL) in the vitreous. These models predict the relative build-up of drug in the vitreous following DDS administration in four different points of origin in the eye. Our model may eventually be used to explore the minimum loading dose of drug required in our DDS leading to reduced drug use and waste.
Photodynamic Therapy Targeting Macrophages Using IRDye700DX-Liposomes Decreases Experimental Arthritis Development
Authors: Dorst Daphne N. , Boss Marti , Rijpkema Mark , Walgreen Birgitte , Helsen Monique M. A. , Bos Desirée L. , van Bloois Louis , Storm Gerrit , Brom Maarten , Laverman Peter , van der Kraan Peter M. , Buitinga Mijke , Koenders Marije I. , Gotthardt Martin
Published: November 2021

Macrophages play a crucial role in the initiation and progression of rheumatoid arthritis (RA). Liposomes can be used to deliver therapeutics to macrophages by exploiting their phagocytic ability. However, since macrophages serve as the immune system’s first responders, it is inadvisable to systemically deplete these cells. By loading the liposomes with the photosensitizer IRDye700DX, we have developed and tested a novel way to perform photodynamic therapy (PDT) on macrophages in inflamed joints. PEGylated liposomes were created using the film method and post-inserted with micelles containing IRDye700DX. For radiolabeling, a chelator was also incorporated. RAW 264.7 cells were incubated with liposomes with or without IRDye700DX and exposed to 689 nm light. Viability was determined using CellTiterGlo. Subsequently, biodistribution and PDT studies were performed on mice with collagen-induced arthritis (CIA). PDT using IRDye700DX-loaded liposomes efficiently induced cell death in vitro, whilst no cell death was observed using the control liposomes. Biodistribution of the two compounds in CIA mice was comparable with excellent correlation of the uptake with macroscopic and microscopic arthritis scores. Treatment with 700DX-loaded liposomes significantly delayed arthritis development. Here we have shown the proof-of-principle of performing PDT in arthritic joints using IRDye700DX-loaded liposomes, allowing locoregional treatment of arthritis.
Conductive Polymers and Their Nanocomposites as Adsorbents in Environmental Applications
Authors: Khan Mohammad Ilyas , Almesfer Mohammed Khaloufa , Elkhaleefa Abubakr , Shigidi Ihab , Shamim Mohammed Zubair , Ali Ismat H. , Rehan Mohammad
Published: November 2021

Proper treatment and disposal of industrial pollutants of all kinds are a global issue that presents significant techno-economical challenges. The presence of pollutants such as heavy metal ions (HMIs) and organic dyes (ODs) in wastewater is considered a significant problem owing to their carcinogenic and toxic nature. Additionally, industrial gaseous pollutants (GPs) are considered to be harmful to human health and may cause various environmental issues such as global warming, acid rain, smog and air pollution, etc. Conductive polymer-based nanomaterials have gained significant interest in recent years, compared with ceramics and metal-based nanomaterials. The objective of this review is to provide detailed insights into different conductive polymers (CPs) and their nanocomposites that are used as adsorbents for environmental remediation applications. The dominant types of CPs that are being used as adsorbent materials include polyaniline (PANI), polypyrrole (Ppy), and polythiophene (PTh). The various adsorption mechanisms proposed for the removal of ODs, HMIs, and other GPs by the different CPs are presented, together with their maximum adsorption capacities, experimental conditions, adsorption, and kinetic models reported.
Layer-by-Layer Encapsulation of Herbicide-Degrading Bacteria for Improved Surface Properties and Compatibility in Soils
Authors: Gal Reut , Perez-Lapid Neriya , Zvulunov Yael , Radian Adi
Published: November 2021

E. coli cells overexpressing the enzyme atrazine chlorohydrolase were coated using layer-by-layer self-assembly. The polymeric coating was designed to improve the surface properties of the cells and create positively charged, ecologically safe, bio-hybrid capsules that can efficiently degrade the herbicide atrazine in soils. The physio-chemical properties of the bacteria/polymer interface were studied as a function of the polymeric composition of the shell and its thickness. Characterization of cell viability, enzyme activity, morphology, and size of the bio-capsules was done using fluorescence spectroscopy, BET and zeta potential measurements and electron microscopy imaging. Out of several polyelectrolytes, the combination of polydiallyldimethylammonium chloride and polysodium 4-styrenesulfonate improved the surface properties and activity of the cells to the greatest extent. The resulting bio-hybrid capsules were stable, well-dispersed, with a net positive charge and a large surface area compared to the uncoated bacteria. These non-viable, bio-hybrid capsules also exhibited a kinetic advantage in comparison with uncoated cells. When added to soils, they exhibited continuous activity over a six-week period and atrazine concentrations declined by 84%. Thus, the concept of layer-by-layer coated bacteria is a promising avenue for the design of new and sustainable bioremediation and biocatalytic platforms.
Synthesis, Characterization and Structure Properties of Biobased Hybrid Copolymers Consisting of Polydiene and Polypeptide Segments
Authors: Politakos Nikolaos , Moutsios Ioannis , Manesi Gkreti-Maria , Moschovas Dimitrios , Abukaev Ainur F. , Nikitina Evgeniia A. , Kortaberria Galder , Ivanov Dimitri A. , Avgeropoulos Apostolos
Published: November 2021

Novel hybrid materials of the PB-b-P(o-Bn-L-Tyr) and PI-b-P(o-Bn-L-Tyr) type (where PB: 1,4/1,2-poly(butadiene), PI: 3,4/1,2/1,4-poly(isoprene) and P(o-Bn-L-Tyr): poly(ortho-benzyl-L-tyrosine)) were synthesized through anionic and ring-opening polymerization under high-vacuum techniques. All final materials were molecularly characterized through infrared spectroscopy (IR) and proton and carbon nuclear magnetic resonance (1H-NMR, 13C-NMR) in order to confirm the successful synthesis and the polydiene microstructure content. The stereochemical behavior of secondary structures (α-helices and β-sheets) of the polypeptide segments combined with the different polydiene microstructures was also studied. The influence of the α-helices and β-sheets, as well as the polydiene chain conformations on the thermal properties (glass transition temperatures, thermal stability, α- and β-relaxation) of the present biobased hybrid copolymers, was investigated through differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and dielectric spectroscopy (DS). The obtained morphologies in thin films for all the synthesized materials via atomic force microscopy (AFM) indicated the formation of polypeptide fibrils in the polydiene matrix.