bluuebunny/crossref_metadata_2025 huggingface dataset:
Blockade of TNF-α/TNFR2 signalling suppresses colorectal cancer and enhances the efficacy of anti-PD1 immunotherapy by decreasing CCR8+T regulatory cells
Authors: Guo Yixian , Xie Feng , Liu Xu , Ke Shouyu , Chen Jieqiong , Zhao Yi , Li Ning , Wang Zeyu , Yi Gang , Shen Yanying , Li Dan , Zhu Chunchao , Zhang Zizhen , Zhao Gang , Lu Hong , Li Bin , Zhao Wenyi
Published: November 2023

The enrichment of regulatory T cells (Tregs) in the tumour microenvironment (TME) has been recognized as one of the major factors in the initiation and development of resistance to immune checkpoint inhibitors. C–C motif chemokine receptor 8 (CCR8), a marker of activated suppressive Tregs, has a significant impact on the functions of Tregs in the TME. However, the regulatory mechanism of CCR8 in Tregs remains unclear. Here, we revealed that a high level of TNF-α in the colorectal cancer (CRC) microenvironment upregulated CCR8 expression in Tregs via the TNFR2/NF-κB signalling pathway and the FOXP3 transcription factor. Furthermore, in both anti-programmed cell death protein 1 (anti-PD1)-responsive and anti-PD1-unresponsive tumour models, PD1 blockade induced CCR8+ Treg infiltration. In both models, Tnfr2 depletion or TNFR2 blockade suppressed tumour progression by reducing CCR8+ Treg infiltration and thus augmented the efficacy of anti-PD1 therapy. Finally, we identified that TNFR2+CCR8+ Tregs but not total Tregs were positively correlated with adverse prognosis in patients with CRC and gastric cancer. Our work reveals the regulatory mechanisms of CCR8 in Tregs and identifies TNFR2 as a promising target for immunotherapy.
Effects of transfluthrin-treated jute and cotton clothing against resistant and susceptible Aedes aegypti (Diptera: Culicidae) in a semifield system
Authors: Ahebwa Alex , Hii Jeffrey , Neoh Kok-Boon , Leepasert Theerachart , Chareonviriyaphap Theeraphap
Published: November 2023

Volatile pyrethroids exert a range of both lethal and behavioral effects on mosquitoes through the passive release of insecticides into the atmosphere. We investigated the protective efficacy (PE) of transfluthrin-treated jute (TI-jute) and cotton (TI-cotton) fabrics, worn at the back of a protective black vest, against laboratory-reared pyrethroid susceptible and resistant strains of Aedes aegypti (L.) in a semifield system (SFS). Each fabric (1,029 cm2) was treated with 1.79 mg/cm2 of transfluthrin as the intervention. Human landing collections were conducted by 2 collectors seated in designated treatment and control compartments of the SFS. The trials were conducted for 41 days, with 16 days partitioned into morning and evening phases. Furthermore, we examined blood feeding behavior and fecundity of the surviving mosquitoes post-exposure. Results showed that in the morning, the PE of TI-jute (49.4%) was higher than that of TI-cotton (36.8%). TI-jute demonstrated a lower PE of 9.6% against the transfluthrin-resistant strain. Remarkably, a significantly higher number of eggs were laid by the transfluthrin-resistant mosquitoes that survived the intervention (36.5 eggs/female) compared to the control group (11.8 eggs/female). These findings suggest that TI-jute can help protect against bites and alter the life traits of Ae. aegypti. The study highlights that the timing of the intervention during the day affected the efficacy of TI-jute and TI-cotton, while sublethal exposure to transfluthrin stimulated egg production in the resistant strain. These are critical challenges that warrant attention in vector control strategies. Investigating this phenomenon in mosquito reproduction necessitates future research at a molecular level.
The apple transcription factor MdZF-HD11 regulates fruit softening by promoting Mdβ-GAL18 expression
Authors: Wang Miaomiao , Wu Yao , Zhan Wenduo , Wang Hao , Chen Ming , Li Tongxin , Bai Tuanhui , Jiao Jian , Song Chunhui , Song Shangwei , Feng Jiancan , Zheng Xianbo
Published: November 2023

Fruit ripening and the associated softening are major determinants of fruit quality and post-harvest shelf life. Although the mechanisms underlying fruit softening have been intensively studied, there are limited reports on the regulation of fruit softening in apples (Malus domestica). Here, we identified a zinc finger homeodomain transcription factor MdZF-HD11that trans-activates the promoter of Mdβ-GAL18, which encodes a pectin-degradation enzyme associated with cell wall metabolism. Both MdZF-HD11 and Mdβ-GAL18 genes were up-regulated by exogenous ethylene treatment and repressed by 1-methylcyclopropene treatment. Further experiments revealed that MdZF-HD11 binds directly to the Mdβ-GAL18 promoter and up-regulates its transcription. Moreover, using transgenic apple fruit calli, we found that overexpression of Mdβ-GAL18 or MdZF-HD11 significantly enhanced β-galactosidase activity, and overexpression of MdZF-HD11 induced the expression of Mdβ-GAL18. We also discovered that transient overexpression of Mdβ-GAL18 or MdZF-HD11 in ‘Golden Delicious’ apple significantly increased the release of ethylene, reduced fruit firmness, promoted the transformation of skin color from green to yellow, and accelerated ripening and softening of the fruit. Finally, the overexpression of MdZF-HD11 in tomato also promoted fruit softening. Collectively, these results indicate that ethylene-induced MdZF-HD11 interacts with Mdβ-GAL18 to promote the post-harvest softening of apple.
VR as a metaleptic possible world of global citizenship embodiment: a cognitive stylistic approach
Authors: Fawzy Rania Magdi
Published: November 2023

Bringing together narrative elements, virtual affordances, and participants’ embodied interactions, virtual reality (VR) movies instantiate new narrative techniques by offering an immersive experience. This study examines virtual narrative beyond mere interactional engagement and extends the phenomenon to include worlding, metaleptic embodiment, and instantiated possible selves. It aims at exploring VR narrative as idiosyncratic cognitive processes, with a special focus on the notions of empathy and emotional involvement as significant elements contributing to this peculiar interactional and cognitive experience. A cognitive stylistic approach is adopted to explain the functional ability of VR technology in transporting participants to alternate worlds and in making them experience a kind of self-transformation. The immersively metaleptic discourse of Baba Yaga is examined as engaging participants in a quest of how to act as morally and socially empathetic and responsible citizens—global citizens. Baba Yaga narrative deploys the narrative discourses of flashbacks, facework, doubly deictic ‘you’, performatives, and imperatives along with material processes to situate participants in a virtual space of actions and doings and hence encourage them to configure their desired self(ves) across different immersive interactions. The global citizen is embodied in the interactive narrative of Baba Yaga, through invoking various storyworld possible selves (SPSs): the feeling self, the responsible self, and the moral self, which encompasses climate activist self and interculturally aware self who manages to get rid of its own cultural biases as the narrative proceeds. Embodied in these selves, participants transform the virtual world into possible worlds of their own passion, agency, choices, hopes, and desires.
How does the presence of bar affects the fueling of supermassive black holes? An IllustrisTNG100 perspective
Authors: Kataria Sandeep Kumar , Vivek M
Published: November 2023

We conducted a statistical study of black hole masses of barred and unbarred galaxies in the IllustrisTNG100 cosmological magnetohydrodynamical simulations. This work aims to understand the role of the bars in the growth of central supermassive black hole mass and its implications on AGN fueling. Our sample consists of 1191 barred galaxies and 2738 unbarred galaxies in the IllustrisTNG100 simulations. To have an unbiased study, we perform our analysis with an equal number of barred and unbarred galaxies by using various controlled parameters like total galaxy mass, stellar mass, gas mass, dark matter halo mass, etc. Except for the stellar mass controlling, we find that the median of the black hole mass distribution for barred galaxies is higher than that of the unbarred ones, indicating that stellar mass is a key parameter influencing the black hole growth. The higher mean accretion rate of the black holes in barred galaxies, averaged since the bar forming epoch (z ∼ 2), explains the higher mean black hole masses in barred galaxies. Further, we also test that these results are unaffected by other environmental processes like minor/major merger histories and neighbouring gas density of black hole. Although the relationship between stellar mass, bar formation, and black hole growth is complex, with various mechanisms involved, our analysis suggests that bars can play a crucial role in feeding black holes, particularly in galaxies with massive stellar discs.
Intensity and velocity oscillations in a flaring active region
Authors: Millar David C L , Fletcher Lyndsay , Joshi Jayant
Published: November 2023

Chromospheric oscillations can give us insight into the physical environment in the solar atmosphere, both in quiet Sun and flaring conditions. Many authors have reported increases in the prevalence of 3-minute oscillations which are thought to be excited by events which impact the chromosphere such as flares. In this study, we utilized the Ca ii 8542 Å line to study the oscillatory behaviour of the chromosphere in an active region which underwent two B-class flares. We analysed oscillations in both intensity and velocity, and found different behaviours in both. Intensity oscillations were most prevalent over the umbrae of sunspots and magnetic pores in the active region, and the extent of the area which contained significant oscillations was found to decrease when comparing times after the flares to before. By measuring the evolution of the magnetic field, we found that this could be because the areas surrounding the umbrae were becoming more ‘penumbral’ with an increase to the magnetic field inclination. Velocity oscillations were found across the active region both before and after the flares but were observed clearly in areas which were brightened by the second flare. By comparing to EUV imaging, it was seen that strong chromospheric velocity oscillations with 3–4-minute periods occurred at the same time and location as a flare loop cooling 30 min after the second flare peak. This could be evidence of disturbances in the loop exciting a response from the chromosphere at its acoustic cut-off frequency.
Search for the wide-orbit massive companion of XO-7b in the follow-up radial-velocity and transit-timing data: no significant clues
Authors: Garai Z , Pribulla T , Komžík R
Published: November 2023

XO-7b is a hot Jupiter transiting a V = 10.52 mag G0V-type star. The planetary system is interesting because the linear slope in the discovery radial-velocity (RV) data indicated a wide-orbit massive companion. In 2020 we started an RV campaign for the system with the main scientific goal to follow-up this linear slope, and to put constraints on the orbital period of the companion. Furthermore, we aimed at refining the system parameters and we wanted to probe transit time variations (TTVs) of XO-7b in order to search for long-term dynamical signs of the companion of XO-7b in the observed-minus-calculated (O-C) data of mid-transit times. Apart from the discovery RVs, we obtained and analysed 20 follow-up RV observations and TESS photometric data. The previously observed significant linear RV slope was not confirmed with the follow-up RV data, where we detected only a marginal linear slope with the opposite trend. If the announced companion really exists, the most convincing explanation is that both RV data sets were collected near its quadrature position. Based on the RVs we estimated the minimum orbital period, which is Porb, min, 3 ≳ 7900 ± 1660 d, and the ‘minimum’ minimum mass of the companion, which is (M3sin i)min = 16.7 ± 3.5 MJup. We did not find significant evidence of the companion of XO-7b in the O-C data set of mid-transit times. We can again conclude that if the announced companion really exists, this is in agreement with previous results that distant companions of exoplanets are only known by RV solutions.
Immediate drop of urine osmolality upon tolvaptan initiation predicts impact on renal prognosis in patients with ADPKD
Authors: Akihisa Taro , Kataoka Hiroshi , Makabe Shiho , Manabe Shun , Yoshida Rie , Ushio Yusuke , Sato Masayo , Yajima Aiji , Hanafusa Norio , Tsuchiya Ken , Nitta Kosaku , Hoshino Junichi , Mochizuki Toshio
Published: November 2023

Background

Tolvaptan, a vasopressin V2 receptor antagonist, is used for treating autosomal dominant polycystic kidney disease (ADPKD). We focused on changes in urinary osmolality (U-Osm) after tolvaptan initiation to determine whether they were associated with the therapeutic response to tolvaptan.

Methods

This was a single-centre, prospective, observational cohort study. Seventy-two patients with ADPKD who received tolvaptan were recruited. We analysed the relationship between changes in U-Osm and annual estimated glomerular filtration rate (eGFR) in terms of renal prognostic value using univariable and multivariable linear regression analyses.

Results

The mean value of U-Osm immediately before tolvaptan initiation was 351.8 ± 142.2 mOsm/kg H2O, which decreased to 97.6 ± 23.8 mOsm/kg H2O in the evening. The decrease in U-Osm was maintained in the outpatient clinic 1 month later. However, the 1-month values of U-Osm showed higher variability (160.2 ± 83.8 mOsm/kg H2O) than did those in the first evening of tolvaptan administration. Multivariate analysis revealed that the baseline eGFR, baseline urinary protein and U-Osm change in the evening of the day of admission (initial U-Osm drop) were significantly correlated with the subsequent annual change in eGFR.

Conclusions

U-Osm can be measured easily and rapidly, and U-Osm change within a short time after tolvaptan initiation may be a useful index for the renal prognosis in actual clinical practice.
Longitudinal drug synergy assessment using convolutional neural network image-decoding of glioblastoma single-spheroid cultures
Authors: Giczewska Anna , Pastuszak Krzysztof , Houweling Megan , Abdul Kulsoom U , Faaij Noa , Wedekind Laurine , Noske David , Wurdinger Thomas , Supernat Anna , Westerman Bart A
Published: November 2023

Background

In recent years, drug combinations have become increasingly popular to improve therapeutic outcomes in various diseases, including difficult to cure cancers such as the brain cancer glioblastoma. Assessing the interaction between drugs over time is critical for predicting drug combination effectiveness and minimizing the risk of therapy resistance. However, as viability readouts of drug combination experiments are commonly performed as an endpoint where cells are lysed, longitudinal drug-interaction monitoring is currently only possible through combined endpoint assays.

Methods

We provide a method for massive parallel monitoring of drug interactions for 16 drug combinations in 3 glioblastoma models over a time frame of 18 days. In our assay, viabilities of single neurospheres are to be estimated based on image information taken at different time points. Neurosphere images taken on the final day (day 18) were matched to the respective viability measured by CellTiter-Glo 3D on the same day. This allowed to use of machine learning to decode image information to viability values on day 18 as well as for the earlier time points (on days 8, 11, and 15).

Results

Our study shows that neurosphere images allow us to predict cell viability from extrapolated viabilities. This enables to assess of the drug interactions in a time window of 18 days. Our results show a clear and persistent synergistic interaction for several drug combinations over time.

Conclusions

Our method facilitates longitudinal drug-interaction assessment, providing new insights into the temporal-dynamic effects of drug combinations in 3D neurospheres which can help to identify more effective therapies against glioblastoma.
Optoelectronic tuning of plasmon resonances via optically modulated hot electrons
Authors: Yao Jiacheng , Wang Cheng , Zhang Chi , Ma Song , Zhou Li , Wang Ti , Wang Ququan , Xu Hongxing , Ding Tao
Published: November 2023

Fast optical modulation of nanoplasmonics is fundamental for on-chip integration of all-optical devices. Although various strategies have been proposed for dynamic modulation of surface plasmons, critical issues of device compatibility and extremely low efficiency in the visible spectrum hamper the application of optoplasmonic nanochips. Here we establish an optoplasmonic system based on Au@Cu2–xS hybrid core–shell nanoparticles. The optical excitation of hot electrons and their charge transfer to the semiconductor coating (Cu2–xS) lead to lowered electron density of Au, which results in the red shift of the localized surface plasmon resonance. The hot electrons can also transport through the Cu2–xS layer to the metal substrate, which increases the conductance of the nanogap. As such, the coupled gap plasmon blue-shifts with a magnitude of up to ∼15 nm, depending on the excitation power and the thickness of the coatings, which agrees with numerical simulations. All of this optoelectronic tuning process is highly reversible, controllable and fast with a modulated laser beam, which is highly compatible and sufficiently useful for on-chip integration of nanophotonic devices.
Digital neuromorphic technology: current and future prospects
Authors: Furber Steve
Published: November 2023

Digital approaches to brain-inspired computing have advanced apace over recent years - where is the state-of-the-art and what does the future hold?
Rapid growth in a large Cambrian apex predator
Authors: Wu Yu , Pates Stephen , Pauly Daniel , Zhang Xingliang , Fu Dongjing
Published: November 2023

Despite the importance of ontogenetic data on early diverging euarthropods to our understanding of the ecology and evolution of past life, the data are distinctly lacking, as reconstructing life histories of fossil animals is often challenging. Here we report the growth trajectory of frontal appendages of the apex predator Amplectobelua symbrachiata, one of the most common radiodont arthropods from the early Cambrian Chengjiang biota (c. 520 Ma) of China. Analysis of 432 specimens (9.1–137.1 mm length; 1.3–25.6 mm height) reveals that appendages grew isometrically, with an estimated maximum size of the whole animal of c. 90 cm. Individuals grew rapidly compared to extant arthropods, as assessed using the electronic length-frequency analysis (ELEFAN) approach. Therefore, we show that the Cambrian apex predator A. symbrachiata was an extremely fast-growing arthropod, with an unusual life history strategy that formed as part of the escalatory ‘arms race’ that shaped the Cambrian explosion over 500 Ma.
Introducing Quin: The Design and Development of a Prototype Chatbot to Support Smoking Cessation
Authors: Bendotti Hollie , Ireland David , Lawler Sheleigh , Oates David , Gartner Coral , Marshall Henry M
Published: November 2023

Introduction

Chatbots emulate human-like interactions and may usefully provide on-demand access to tailored smoking cessation support. We have developed a prototype smartphone application-based smoking cessation chatbot, named Quin, grounded in real-world, evidence-, and theory-based smoking cessation counseling sessions.

Methods

Conversation topics and interactions in Quitline counseling sessions (N = 30; 18 h) were characterized using thematic, content, and proponent analyses of transcripts. Quin was created by programming this content using a chatbot framework which interacts with users via speech to text. Reiterative changes and additions were made to the conversation structure and dialogue following regular consultation with a multidisciplinary team from relevant fields, and from evidence-based resources.

Results

Chatbot conversations were encoded into initial and scheduled follow-up “appointments.” Collection of demographic information, and smoking and quit history, informed tailored discussion about pharmacotherapy preferences, behavioral strategies, and social and professional support to form a quit plan. Follow-up appointments were programmed to check in on user progress, review elements of the quit plan, answer questions, and solve issues. Quin was programmed to include teachable moments and educational content to enhance health literacy and informed decision-making. Personal agency is encouraged through exploration and self-reflection of users’ personal behaviors, experiences, preferences, and ideas.

Conclusions

Quin’s successful development represents a movement toward improving access to personalized smoking cessation support. Qualitative foundations of Quin provide greater insight into the smoking cessation counseling relationship and enhances the conversational ability of the technology. The prototype chatbot will be refined through beta-testing with end users and stakeholders prior to evaluation in a clinical trial.

Implications

Our novel study provides transparent description of the translation of qualitative evidence of real-world smoking cessation counseling sessions into the design and development of a prototype smoking cessation chatbot. The successful iterative development of Quin not only embodies the science and art of health promotion, but also a step forward in expanding the reach of tailored, evidence based, in-pocket support for people who want to quit smoking.
Racial Polarization in Attitudes towards the Criminal Legal System
Authors: Lee Karen Hanhee , Gutierrez Carmen , Pettit Becky
Published: November 2023

Existing research often views attitudes toward the U.S. criminal legal system as reflections of punitive sentiment, overlooking racial differences in how people respond to questions related to crime and punishment. Using over four decades of nationally representative survey data from the General Social Survey, we employ latent class analysis to examine racial variation in attitudes about the U.S. criminal legal system across time. We find that among White Americans, support for increased spending to combat crime corresponds with support for harsher courts and the death penalty. In contrast, many Black Americans support increased spending on crime but oppose harsher courts and the death penalty, indicating simultaneous concern about crime and a more punitive criminal legal system. Although aggregate trends in punitiveness change similarly across race and time, we show that while preferences for punitive policies remain high among White Americans, the proportion of Black Americans who are simultaneously concerned about crime and a punitive criminal legal system rose from 14 percent in 1994 to 56 percent in 2018. These results highlight the salience of race in shaping how people evaluate the criminal legal system and draw attention to racial polarization in views on punishment and justice.
Household Debt and Social Reproduction in Everyday Life: Women’s Experiences of Caring, Agency, and Risk
Authors: Kılınçarslan Pelin
Published: November 2023

This article explores the ways in which everyday life produces gendered links between debt and social reproduction in contradictory ways. Based on interviews with women from indebted households in Athens and Istanbul, it argues that debt and socially reproductive work come to rely on one another, with gendered implications for caring, expanded agency, and embodied risks. Indebtedness demands forms of socially reproductive labor that women practice through caring for the debt and the indebted family. While this expands women’s agency, it also reinforces their experiences of distress and social isolation. This dual outcome reveals gendered contradictions emerging through the interdependency of debt and social reproduction. While the management of debt relies on socially reproductive labor through which women exercise greater agency, it creates embodied risks that threaten their own social reproduction, which also relies on debt.
Sstr2 Defines the Cone Differentiation-Competent Late-Stage Retinal Progenitor Cells in the Developing Mouse Retina
Authors: Bai Yihan , He Han , Ren Bangqi , Ren Jiayun , Zou Ting , Chen Xi , Liu Yong
Published: November 2023

Cone cell death is a characteristic shared by various retinal degenerative disorders, such as cone-rod dystrophy, Stargardt disease, achromatopsia, and retinitis pigmentosa. This leads to conditions like color blindness and permanently impaired visual acuity. Stem cell therapy focused on photoreceptor replacement holds promise for addressing these conditions. However, identifying surface markers that aid in enriching retinal progenitor cells (RPCs) capable of differentiating into cones remains a complex task. In this study, we employed single-cell RNA sequencing to scrutinize the transcriptome of developing retinas in C57BL/6J mice. This revealed the distinctive expression of somatostatin receptor 2 (Sstr2), a surface protein, in late-stage RPCs exhibiting the potential for photoreceptor differentiation. In vivo lineage tracing experiments verified that Sstr2+ cells within the late embryonic retina gave rise to cones, amacrine and horizontal cells during the developmental process. Furthermore, Sstr2+ cells that were isolated from the late embryonic mouse retina displayed RPC markers and exhibited the capability to differentiate into cones in vitro. Upon subretinal transplantation into both wild-type and retinal degeneration 10 (rd10) mice, Sstr2+ cells survived and expressed cone-specific markers. This study underscores the ability of Sstr2 to enrich late-stage RPCs primed for cone differentiation to a large extent. It proposes the utility of Sstr2 as a biomarker for RPCs capable of generating cones for transplantation purposes.
Ability of seedlings to survive heat and drought portends future demographic challenges for five southwestern US conifers
Authors: Crockett Joseph L , Hurteau Matthew D
Published: November 2023

Climate change and disturbance are altering forests and the rates and locations of tree regeneration. In semi-arid forests of the southwestern USA, limitations imposed by hot and dry conditions are likely to influence seedling survival. We examined how the survival of 1-year seedlings of five southwestern US conifer species whose southwestern distributions range from warmer and drier woodlands and forests (Pinus edulis Engelm., Pinus ponderosa Douglas ex C. Lawson) to cooler and wetter subalpine forests (Pseudotsuga menziesii (Mirb.) Franco, Abies concolor (Gord. & Glend.) Lindl. Ex Hildebr. and Picea engelmannii Parry ex Engelm.) changed in response to low moisture availability, high temperatures and high vapor pressure deficit in incubators. We used a Bayesian framework to construct discrete-time proportional hazard models that explained 55–75% of the species-specific survival variability. We applied these to the recent climate (1980–2019) of the southwestern USA as well as 1980–2099 CMIP5 climate projections with the RCP8.5 emissions pathway. We found that the more mesic species (i.e., P. menziesii, A. concolor and P. engelmannii) were more susceptible to the effects of hot and dry periods. However, their existing ranges are not projected to experience the conditions we tested as early in the 21st century as the more xeric P. edulis and P. ponderosa, leading to lower percentages of their existing ranges predicted to experience seedling-killing conditions. By late-century, extensive areas of each species southwestern range could experience climate conditions that increase the likelihood of seedling mortality. These results demonstrate that empirically derived physiological limitations can be used to inform where species composition or vegetation type change are likely to occur in the southwestern USA.
Contrasting water-use strategies revealed by species-specific transpiration dynamics in the Caatinga dry forest
Authors: Wright Cynthia L , West Jason B , de Lima André L A , Souza Eduardo S , Medeiros Maria , Wilcox Bradford P
Published: November 2023

In forest ecosystems, transpiration (T) patterns are important for quantifying water and carbon fluxes and are major factors in predicting ecosystem change. Seasonal changes in rainfall and soil water content can alter the sensitivity of sap flux density to daily variations in vapor pressure deficit (VPD). This sensitivity is species-specific and is thought to be related to hydraulic strategies. The aim of this work is to better understand how the sap flux density of species with low versus high wood density differ in their sensitivity to VPD and soil water content and how potentially opposing water-use strategies influence T dynamics, and ultimately, correlations to evapotranspiration (ET). We use hysteresis area analysis to quantify the sensitivity of species-specific sap flux density to changes in the VPD, breakpoint-based models to determine the soil water content threshold instigating a T response and multiscalar wavelet coherency to correlate T to ET. We found that low wood density Commiphora leptophloeos (Mart.) Gillett had a more dynamic T pattern, a greater sensitivity to VPD at high soil water content, required a higher soil water content threshold for this sensitivity to be apparent, and had a significant coherency correlation with ET at daily to monthly timescales. This behavior is consistent with a drought avoidance strategy. High wood density Cenostigma pyramidale  (Tul.)  E. Gagnon & G. P. Lewis, conversely, had a more stable T pattern, responded to VPD across a range of soil water content, tolerated a lower soil water content threshold to T, and had a significant coherency correlation with ET at weekly timescales. This behavior is consistent with a drought-tolerant strategy. We build on previous research to show that these species have contrasting water-use strategies that should be considered in large-scale modeling efforts.
Integrative taxonomy reveals a new gall midge genus and species (Diptera: Cecidomyiidae) developing in the flower buds of Pongamia pinnata (Fabaceae) in Japan
Authors: Elsayed Ayman Khamis
Published: November 2023

The Ryukyu Archipelago of Japan is a biodiversity hotspot with a remarkable diversity of endemic plants and animals. Although recent biodiversity surveys have highlighted that gall midges (Diptera: Cecidomyiidae) are the most species-rich taxon of insects worldwide, only 43 species are known presently in the Ryukyus. In this study, an undescribed gall midge developing in the flower buds of Pongamia pinnata (Fabaceae) was found on Ishigaki Island, southern Ryukyus, Okinawa Prefecture, Japan. Morphologically, the gall midge belongs to the supertribe Cecidomyiidi (Cecidomyiidae: Cecidomyiinae), but it does not fit the definition of any of the tribes and genera classified of Cecidomyiidi. A molecular phylogenetic analysis including the main tribes of Cecidomyiidi was conducted and reinforced the results of morphological examination. Therefore, Ishigakidiplosis karamaeElsayed,gen. nov. et sp.nov. was introduced for what is no doubt a previously unnamed species. This new genus sheds light on the unique gall midges of the Ryukyus and highlights the importance of conducting further surveys to elucidate their diversity and evolutionary relationships to other known gall midges.
Magnetically Controlled Growing Rods Graduation: Lessons From a Single-center Series of 48 Patients
Authors: Gurel Ron , Elbaz Etay , Sigal Amit , Gigi Roy , Otremski Hila , Lebel David E. , Ovadia Dror
Published: November 2023

Background:

Magnetically controlled growing rod (MCGR) treatment for early-onset scoliosis (EOS) has become a widely utilized method of deformity control, but long-term follow-up reports are sparse. We evaluated the outcomes of a large group of consecutive pediatric patients diagnosed with EOS who were treated by MCGR to the endpoint of definitive spinal fusion in a single center. We hypothesized that lessons learned from treating a large volume of MCGR patients would lower the complication rate in comparison to what was previously reported by other studies.

Methods:

The records of 48 EOS patients with varied etiologies who were treated by MCGR between 2012 and 2022 and reached the end of treatment were analyzed retrospectively for baseline and final radiographic measurements and surgery-related complications.

Results:

The mean percent of improvement in the major coronal deformity was 51.7±25.0% and the mean T1 to T12 total growth was 52.9±21.9 mm. The total complication rate was 22.9% of which 12.5% required a single unplanned surgery, 6.3% required multiple unplanned surgeries, and 4% did not require any unplanned surgery.

Conclusion:

MCGR treatment provides adequate control of EOS, enabling satisfactory growth of the thoracic spine. The complication rate in a single large volume center is moderate.

Level of Evidence:

IV.
Manipulation Under Anesthesia is Safe and Effective for Management of Early Postoperative Knee Arthrofibrosis in Adolescent Patients
Authors: Marquez-Lara Alejandro , Padget William , Wall Eric J. , Parikh Shital N.
Published: November 2023

Background:

Management of postoperative knee arthrofibrosis can be challenging and the preferred time for intervention remains controversial. The purpose of this study is to evaluate the safety and efficacy of early (<3 mo postoperatively) manipulation under anesthesia (MUA) for the treatment of knee arthrofibrosis in adolescent patients. We hypothesized that early MUA could restore normal knee motion with a low complication rate and without the need for more invasive intervention.

Methods:

In a retrospective review, 57 patients who underwent MUA for postoperative knee arthrofibrosis were identified. The time between the index surgery and MUA as well as changes in range of motion (ROM) before and after MUA were analyzed. Descriptive statistics with median and interquartile range were used to analyze this non-parametric study cohort. Repeated measures ANOVA was performed to assess improvement in ROM over time. A P value <0.05 denoted statistical significance.

Results:

The median age of the cohort at time of MUA was 14.5 years [interquartile range (IQR) 12.9 to 17.6)]. 54.4% were male. Median time to MUA was 64 days (IQR 52 to 79) after index surgery. ROM before MUA was 90.0 degrees (IQR 75 to 100), which improved to 130 degrees (120 to 135) after MUA. At final median follow-up of 8.9 months (IQR 5.1 to 16.1), mean ROM was 133 degrees (130 to 140). There were no iatrogenic fractures or physeal separations associated with MUA. 12.3% (n=7/57) failed MUA either due to the need for subsequent repeat MUA (n=2), need for lysis of adhesions (n=3) or need for surgery after MUA (n=2). Those who failed early MUA and required subsequent procedures had ROM >120 degrees at final follow-up.

Conclusions:

Postoperative knee arthrofibrosis can be safely and effectively treated with early (<3 mo postoperative) MUA. There were no iatrogenic fractures or physeal separations during MUA. Patients who had recurrence of motion deficits after early MUA and required further intervention, regained satisfactory knee motion at final follow-up. Although further research is warranted to better characterize risk factors for knee arthrofibrosis in adolescent patients, early recognition and MUA is a safe and effective treatment for arthrofibrosis to help patients regain full ROM without invasive intervention.

Level of Evidence:

Therapeutic Study – Level IV.
Cabozantinib inhibits HBV-RNA transcription by decreasing STAT3 binding to the enhancer region of cccDNA
Authors: Funato Kazuyoshi , Miyake Nozomi , Sekiba Kazuma , Miyakawa Yu , Seimiya Takahiro , Shibata Chikako , Kishikawa Takahiro , Otsuka Motoyuki
Published: November 2023

Background:

Precision medicine and customized therapeutics based on the features of each patient are important for maximizing therapeutic effects. Because most cases of HCC occur in the damaged liver through various etiologies, such as hepatitis virus infection, steatohepatitis, and autoimmune hepatitis, there should be a rationale for the choice of therapeutic options based on these etiologies. Although cabozantinib, an oral multikinase inhibitor, has demonstrated clinical effectiveness in advanced HCC, subgroup analyses showed a lower HR for death in HBV-related HCC. This study aimed to determine the therapeutic effects of cabozantinib in HBV-related HCC.

Methods:

Using HBV infection models and gene knockout cells, we determined the crucial signaling axis responsible for the effects of cabozantinib on HBV. A chromatin immunoprecipitation assay was performed to determine the interaction between the signaling molecules and HBV DNA. Agonists and inhibitors were used for confirmation.

Results:

Cabozantinib inhibited HBV replication through the HGF-mesenchymal-epithelial transition factor–signal transducer and activator of transcription 3 (MET-STAT3) signaling axis. The importance of STAT3 in viral replication has been confirmed using gene-edited STAT3 knockout cells. The chromatin immunoprecipitation assay revealed that the binding levels of phosphorylated STAT3 to enhancer region 1 of HBV covalently closed circular DNA were significantly increased by HGF stimulation.

Conclusions:

Cabozantinib has favorable therapeutic effects on HBV-related HCC because it inhibits HCC not only directly but also indirectly by means of inhibitory effects on HBV.
Left atrial mechanics evaluated by two-dimensional strain analysis: alterations in essential hypertension
Authors: Stefani Luke D. , Trivedi Siddharth J. , Ferkh Aaisha , Emerson Peter , Marschner Simone , Gan Gary , Altman Mikhail , Thomas Liza
Published: November 2023

Background:

Hypertension is a cardiovascular risk factor that predisposes to cardiac structural alterations namely increased left ventricular (LV) wall thickness, reduced LV compliance and diastolic dysfunction, with consequent left atrial (LA) dilation and functional impairment. In this article, we evaluated differences in left atrial structure and function using two-dimensional speckle tracking echocardiography in patients with hypertension compared with controls.

Methods:

This was a retrospective cross-sectional study of 208 hypertensive patients and 157 controls who underwent a comprehensive transthoracic echocardiogram. Patients with hypertension were stratified by the presence of left ventricular hypertrophy (LVH).

Results:

Non-LVH hypertension patients had lower left atrial reservoir strain (LASRES) (34.78 ± 29.78 vs. 29.78 ± 6.08; P = 0.022) and conduit strain (LASCD) (19.66 ± 7.29 vs. 14.23 ± 4.59; P = 0.014) vs. controls despite similar left atrial volumes (LAV). Left atrial contractile strain (LASCT) was not significantly different between non-LVH hypertension patients and controls (15.12 ± 3.77 vs. 15.56 ± 3.79; P = 0.601). Left atrial mechanical dispersion was significantly higher in the LVH group compared with the non-LVH hypertension group (42.26 ± 13.01 vs. 50.06 ± 14.95; P = 0.009). In multivariate regression analysis, LVH correlated with left atrial mechanical dispersion (P = 0.016). An age–hypertension interaction independently correlated with LASCT (P < 0.001).

Conclusion:

Hypertension results in functional left atrial changes even before development of LV hypertrophy and structural left atrial changes with increased left atrial volume. We demonstrate both a likely hypertension-associated left atrial myopathy that prevents age-related compensatory increase in left atrial contractile function, and impact of LVH in hypertension on left atrial dyssynchrony.
Metal ions as effectual tools for cancer with traditional Chinese medicine
Authors: Wang Lei , Xie Yingqiu , Myrzagali Sandugash , Pu Weiling , Liu Erwei
Published: November 2023

Malignant tumor has become a major threat affecting human health, and is one of the main causes of human death. Recent studies have shown that many traditional Chinese medicines (TCM) have good anti-tumor activity, which may improve the therapeutic effect of routine treatment and quality of life with lower toxicity. However, the efficacy of TCM alone for the treatment of tumors is limited. Metal ions are essential substances for maintaining normal physiological activities. This article summarized the multiple mechanisms in which metal ions are involved in the prevention and treatment of tumors in TCM.
Effects of Walking Combined With Resistance Band Exercises on Alleviating Cancer-Related Fatigue
Authors: Liu An-I , Lee Yun-Hsiang , Lu Chi-Yu , Huda Nurul , Huang Tsai-Wei
Published: November 2023

Background

Cancer-related fatigue (CRF) is a common symptom, and exercise has shown potential in alleviating CRF. However, there is a need for diverse exercise options tailored to individual patient needs.

Objective

To evaluate the overall effects of a combined walking and resistance band exercise intervention in relieving CRF among cancer patients through randomized controlled trials.

Methods

Comprehensive searches were conducted in multiple databases to identify relevant studies up until March 2023. Inclusion criteria required the intervention to involve walking combined with elastic band training, with a clear exercise protocol description. The primary outcome was CRF, and secondary outcomes included walking steps, distance, mood distress, and quality of life. Standardized mean differences (SMDs) with 95% confidence intervals (CIs) were calculated using random-effects models.

Results

Ten trials were included. The intervention group showed significant improvements in CRF (SMD, −0.40; 95% CI, −0.60 to −0.20), mood distress (SMD, −0.30; 95% CI, −0.53 to −0.07), and daily walking steps (SMD, 0.52; 95% CI, 0.07-0.96) compared with the control group. Although the 6-Minute Walk Test and quality of life did not show significant differences, a trend toward improvement was observed in the intervention group. Adverse events related to the intervention were infrequent.

Conclusion

A combined walking and resistance band exercise intervention can effectively alleviate CRF and improve mood distress and daily walking steps among cancer patients.

Implications for Practice

This exercise option may provide an additional strategy to manage CRF. Further research is needed to explore the optimal exercise prescription for individual patients.
Nurse Managers' Perceived Self-leadership Levels
Authors: Ma Holly
Published: November 2023

OBJECTIVE

The aim of this study was to determine the perceived levels of self-leadership among nurse managers in the United States.

BACKGROUND

Self-leadership is the ability to influence and engage oneself to be self-aware and responsible, and leverage strengths to perform. Despite benefits identified from extant literature, there are no studies on self-leadership among nurse managers in the United States.

METHODS

A cross-sectional descriptive design was used. Eighty-eight nurse managers completed a survey using the Revised Self-Leadership Questionnaire.

RESULTS

Nurse managers identified moderate levels of self-leadership; the strategies with the lowest scores were constructive thought strategies, self-reward behaviors, and visualizing performance. There was a relationship between age and the identified self-leadership levels.

CONCLUSIONS

Further studies are needed to understand self-leadership among nurse leaders in the United States and beyond. There is an opportunity to increase nurse managers' self-leadership level by focusing on interventions to increase constructive thought pattern strategies, self-reward behaviors, and visualizing successful performance.
Solitary Pulmonary Fibroleiomyomatous Hamartoma on 18F-FDG PET/CT
Authors: Tao Yi , Wang Feifei , Yan Jiao , Chen Zhengguo , He Chuandong
Published: November 2023

Solitary pulmonary fibroleiomyomatous hamartoma is a very rare benign tumor. A 57-year-old man presented with dry cough over 1 month. A CT showed an irregular nodule in the left upper lobe of the lung. A malignant tumor was suspected, and further investigation with 18F-FDG PET/CT showed the pulmonary nodule with slightly increased FDG uptake. Thoracoscopic resection was performed. Subsequent histopathological and immunohistochemical tests confirmed the diagnosis of fibroleiomyomatous hamartoma.
The Craniofacial Collaboration UK: Developmental Outcomes in 7- and 10-Year-Old Children With Metopic Synostosis
Authors: Edwards-Bailey Laura , Piggott Katie , Dalton Louise , Horton Jo , Parks Christopher , White Samuel , Wright Gillian , Kearney Anna
Published: November 2023

The Craniofacial Collaboration (CC-UK) is a shared initiative across the Psychology teams attached to 4 highly specialized craniofacial centers in the United Kingdom. The CC-UK aims to address key limitations in the existing craniofacial literature by analyzing data for homogenous samples of children with craniosynostosis. This article presents the fifth wave of CC-UK data collection, focused on 7- and 10-year olds who have undergone primary corrective surgery for metopic synostosis (MS). Data for children with sagittal synostosis and MS have previously been presented at 3 and 5 years. This paper continues to build on this with consideration to older age groups, presenting the first CC-UK analysis of cognitive assessment data using the Wechsler Abbreviated Scale of Intelligence—Second Edition. Results show that the majority of children with MS fall within the average ranges across behavioral and neurodevelopmental domains. However, several domains indicated a trend of heightened concern when compared with normative data, particularly for parent-reported outcomes, suggesting that there may be some subtle difficulties for children with MS. Consideration of how these findings compare with that of previous CC-UK analyses is explored. Further, implications for clinical practice and future research are considered, with the need for longitudinal analyses, as well as data from multiple perspectives (eg, school, parents, and self) at older age points to establish patterns over time. Through collaboration across the highly specialized craniofacial centers, the CC-UK hopes to work toward this goal moving forward.
Akinetopsia: A Systematic Review
Authors: Van Swol Joshua M. , Thompson Elizabeth B. , Joffe Jonah A. , Nguyen Shaun A. , Berman Eric L.
Published: November 2023

Selective motion blindness, also known as akinetopsia, is infrequently reported in the literature. Hence, little is known about the condition including its causes, time course, pathophysiology, and current diagnostic methods. In this investigation, we comprehensively surveyed the literature using a systematic review to identify each reported case of the condition. The purpose of this study was to provide an exhaustive catalog of every published occurrence to date to identify and discuss trends, commonalities, and differences among them. Our results revealed distinct characteristics for the various etiologies of this phenomenon in addition to a shared pathophysiologic pathway among them.