bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of Distributed Acoustic Sensing in Geophysics Exploration: Comparative Review of Single-Mode and Multi-Mode Fiber Optic Cables
Authors: Rafi Muhammad , Mohd Noh Khairul Arifin , Abdul Latiff Abdul Halim , Otchere Daniel Asante , Tackie-Otoo Bennet Nii , Putra Ahmad Dedi , Riyadi Zaky Ahmad , Asfha Dejen Teklu
Published: June 2024

The advent of fiber optic technology in geophysics exploration has grown in its use in the exploration, production, and monitoring of subsurface environments, revolutionizing the way data are gathered and interpreted critically to speed up decision-making and reduce expense and time. Distributed Acoustic Sensing (DAS) has been increasingly utilized to build relationships in complex geophysics environments by utilizing continuous measurement along fiber optic cables with high spatial resolution and a frequency response of up to 10 KHz. DAS, as fiber optic technology examining backscattered light from a laser emitted inside the fiber and measuring strain changes, enables the performance of subsurface imaging in terms of real-time monitoring for Vertical Seismic Profiling (VSP), reservoir monitoring, and microseismic event detection. This review examines the most widely used fiber optic cables employed for DAS acquisition, namely Single-Mode Fiber (SMF) and Multi-Mode Fiber (MMF), with the different deployments and scopes of data used in geophysics exploration. Over the years, SMF has emerged as a preferred type of fiber optic cable utilized for DAS acquisition and, in most applications examined in this review, outperformed MMF. On the other side, MMF has proven to be preferable when used to measure distributed temperature. Finally, the fiber optic cable deployment technique and acquisition parameters constitute a pivotal preliminary step in DAS data preprocessing, offering a pathway to improve imaging resolution based on DAS measurement as a future scope of work.
Recent Innovations Brought about by Weight-Bearing CT Imaging in the Foot and Ankle: A Systematic Review of the Literature
Authors: Lintz François , de Cesar Netto Cesar , Belvedere Claudio , Leardini Alberto , Bernasconi Alessio ,   on behalf of the International Weight-Bearing CT Society
Published: June 2024

The decade from 2010–2020 has seen the development of cone beam weight-bearing CT (WBCT) as a major innovation in the foot and ankle realm, becoming an important modality for bone and joint imaging. The ability to provide three-dimensional images of the naturally loaded skeleton has enabled several subsequent innovations to arise with aims to hasten image processing and to extend the clinical applications of WBCT. The objective of this work was to identify, categorize and explain those emerging techniques. We performed a structured review of the literature according to PRISMA standards, finally including 50 studies. We subsequently proposed a classification of these techniques. Segmentation and distance mapping were identified as key features. We conclude that although WBCT has already been adopted in a number of clinical communities with an immediate improvement in patient workflows, adoption of advanced techniques is yet to come. However, that relies mostly not on the technology itself, but on improvements in AI software allowing practitioners to quickly process images in daily practice and enabling the clinicians to obtain an accurate three-dimensional evaluation of the segment considered. Standardization will be paramount to amass large amounts of comparable data, which will fuel further innovations in a potentially virtuous circle.
Exact Mathematical Solution for Maximum Transient Offtracking Calculation of a Single-Unit Vehicle Negotiating Circular Curves
Authors: Ilić Vladan , Lukić Miloš , Gavran Dejan , Fric Sanja , Trpčevski Filip , Vranjevac Stefan , Milovanović Nikola
Published: June 2024

The low-speed turning maneuverability of vehicles is closely related to a well-known offtracking phenomenon which occurs when the rear wheels of a turning vehicle deviate towards the inside of a horizontal curve. Although numerous mathematical models and computer programs for vehicle swept path analysis have been developed in the past, only a few of them can calculate the maximum transient offtracking of a turning vehicle, yet with limited accuracy. The authors were motivated by this fact to find a new mathematical solution for maximum transient offtracking calculation of a single-unit vehicle when negotiating circular curves. In the first stage, a transcendental equation defining vehicle maximum transient offtracking position is derived and numerically solved by Python 3.10.12. In the second stage, the polynomial regression model predicting accurate numerical solutions of the transcendental equation with the desired level of accuracy was developed and tested. The new calculation method is simple enough to simply take the vehicle datum length, circular curve radii, and turn angle, while instantly producing the maximum transient offtracking value, without the need to draw any of the vehicle movement trajectories.
Monogenity and Power Integral Bases: Recent Developments
Authors: Gaál István
Published: June 2024

Monogenity is a classical area of algebraic number theory that continues to be actively researched. This paper collects the results obtained over the past few years in this area. Several of the listed results were presented at a series of online conferences titled “Monogenity and Power Integral Bases”. We also give a collection of the most important methods used in several of these papers. A list of open problems for further research is also given.
Comparative Analysis of Exact Methods for Testing Equivalence of Prevalences in Bilateral and Unilateral Combined Data with and without Assumptions of Correlation
Authors: Liang Shuyi , Ma Changxing
Published: June 2024

In clinical studies focusing on paired body parts, diseases can manifest on either both sides (bilateral) or just one side (unilateral) of the organs. Consequently, the data in these studies may consist of records from both bilateral and unilateral cases. There are two different methods of analyzing the data. One of the methods is assuming that the pair of measurements from the same subject are independent, while the other considers the correlation between paired organs. In terms of the homogeneity test of proportions, asymptotic methods have been proposed given the moderate size of data. This article extends the existing work by proposing exact methods to deal with the scenarios when the sample size is small and asymptotic methods perform poorly. The impact of the correlation assumption is also explored. Among the proposed methods, calculating p-values by replacing unknown parameters with estimated values while accounting for the correlation is recommended based on its satisfactory type I error controls and statistical powers. The proposed methods are applied to three real examples for illustration.
Ethnobotany of Zmeyovski pelin: A Traditional Artemisia-Flavored Wine from Bulgaria
Authors: Boneva Vanya , Ivanova Teodora , Grozeva Neli , Slavcheva Mariya , Dimitrova Dessislava
Published: June 2024

Flavoring wine with Artemisia species, together with other aromatic herbs and/or fruits, is a practice that has evolved in different parts of Bulgaria. The aromatized wine-based beverage, called pelin, is named after the Bulgarian common name for the genus Artemisia. In this paper, we present Zmeyovski pelin, the history of which dates back to the early 20th century. We employed semi-structured interviews with 15 pelin producers from the Zmeyovo village with an age span from 44 to 83 years. The relative frequency of citation for each stated taxon was recorded. We explored the changes in the plant knowledge of the pelin makers and discussed the drivers for it. From a modern perspective, pelin can be considered a functional beverage since the aromatic plants and fruits adds a variety of biologically active substances to it, thus enhancing its health benefits. We regard the consumers’ inquiry about the benefits of the added plants as a driver for mitigating the plant awareness disparity among the local community.
Poly(2-Hydroxyethyl Methacrylate) Hydrogel-Based Microneedles for Bioactive Release
Authors: Sharma Manoj B. , Abdelmohsen Hend A. M. , Kap Özlem , Kilic Volkan , Horzum Nesrin , Cheneler David , Hardy John G.
Published: June 2024

Microneedle arrays are minimally invasive devices that have been extensively investigated for the transdermal/intradermal delivery of drugs/bioactives. Here, we demonstrate the release of bioactive molecules (estradiol, melatonin and meropenem) from poly(2-hydroxyethyl methacrylate), pHEMA, hydrogel-based microneedle patches in vitro. The pHEMA hydrogel microneedles had mechanical properties that were sufficiently robust to penetrate soft tissues (exemplified here by phantom tissues). The bioactive release from the pHEMA hydrogel-based microneedles was fitted to various models (e.g., zero order, first order, second order). Such pHEMA microneedles have potential application in the transdermal delivery of bioactives (exemplified here by estradiol, melatonin and meropenem) for the treatment of various conditions.
Evaluation of Various Inoculation Methods on the Effect of Beauveria bassiana on the Plant Growth of Kiwi and on Halyomorpha halys Infestation: A Two-Year Field Study
Authors: Papantzikos Vasileios , Mantzoukas Spiridon , Eliopoulos Panagiotis A. , Servis Dimitrios , Bitivanos Stergios , Patakioutas George
Published: June 2024

In this study, the bioinsecticidal action of a commercial formulation with Beauveria bassiana was evaluated on the new sucking pest in Greece: Halyomorpha halys, of the kiwifruit. Additionally, the biostimulant potential of the same formulation was studied on kiwi growth. The application was performed in three different ways in a commercial field of kiwi crop A. deliciosa “Hayward” field in Arta, Greece: (i) trunk spray, (ii) root injection, and (iii) trunk inoculation. During the 2 years seasons of the experiment, weekly measurements of the H. halys population were determined. The insect is sucking plants nutrients; therefore, the total chlorophyll content in the leaves of the treatments was recorded weekly. In addition, the percentage of infested kiwifruits was estimated at the end of the experiment. Moreover, to study the biostimulant potential of the formulation, growth measurements on stems and leaves were performed during the experiment. Finally, at the kiwi harvest point, the fruit biomass, dimensions, and weight were obtained, and the leaves’ proline content was evaluated. The results encourage us to further study this EPF formulation as the bioinsecticidal effect was noted by the reduction in H. halys population, and biostimulant action was perceived by the higher plant biomass.
Insulin Resistance/Hyperinsulinemia as an Independent Risk Factor That Has Been Overlooked for Too Long
Authors: Fazio Serafino , Affuso Flora , Cesaro Arturo , Tibullo Loredana , Fazio Valeria , Calabrò Paolo
Published: June 2024

Unfortunately, cardiovascular diseases and cancers are still the leading causes of death in developed and developing countries despite the considerable progress made in the prevention and treatment of diseases. Maybe we missed something? Insulin resistance (IR) with associated hyperinsulinemia (Hypein) is a silent pandemic whose prevalence is continually growing in developed and developing countries, now exceeding 51% of the general population. IR/Hypein, despite the vast scientific literature supporting its adverse action on the development of type 2 diabetes, cardiovascular alterations, tumors, neurological disorders, and cellular senescence, is not yet considered an independent risk factor and, therefore, is not screened in the general population and adequately treated. There are now numerous substances, drugs, and natural substances that, in association with the correction of a wrong lifestyle, can help to reduce IR/Hypein. We are convinced that the time has come to implement a prevention plan against this critical risk factor. Therefore, this manuscript aims to highlight IR/Hypein as an independent risk factor for type 2 diabetes, cardiovascular diseases, cancers, cellular senescence, and neuropsychiatric disorders, supporting our conviction with the available scientific literature on the topic.
Protective Effect of Hyperprolactinemia on Oxidative Stress in Patients with Psychotic Disorder on Atypical Antipsychotics Risperidone and Paliperidone: A Cross-Sectional Study
Authors: Stojkovic Milena , Jovanovic Mirjana , Jakovljevic Vladimir , Zivkovic Vladimir , Djordjevic Natasa , Kocovic Aleksandar , Nikolic Marina , Stojanovic Aleksandra , Minic Natasa , Ignjatovic Vesna , Vukomanovic Vladimir , Nastic Danijela , Zdravkovic Natasa , Radmanovic Olivera , Djordjic Milan , Babic Sasa , Radmanovic Branimir
Published: June 2024

Several studies indicate the impact of antipsychotics like risperidone and paliperidone on oxidative stress parameters, yet data remain inconsistent. We investigated the link between these medications, hyperprolactinemia (HPRL), and oxidative stress. This study was conducted at the Psychiatry Clinic, University Clinical Center, Kragujevac, between November 2022 and August 2023. Inclusion criteria comprised diagnosed psychotic disorders from the ICD-10-based F20-F29 spectrum and clinical stability on risperidone/paliperidone for ≥12 weeks with no recent dose adjustments. Exclusion criteria included pregnancy, breastfeeding, relevant medical conditions, or co-therapy with prolactin-secreting drugs. Data encompassed drug choice, administration method, therapy duration, and daily dose. Prolactin (PRL) levels, oxidative stress parameters (TBARS, H2O2, O2−, NO2−), and antioxidant system (CAT, GSH, SOD) were assessed. Of 155 subjects, women exhibited significantly higher PRL levels (p < 0.001) and symptomatic HPRL (p < 0.001). Drug choice and regimen significantly influenced TBARS (p < 0.001), NO2− (p < 0.001), O2− (p = 0.002), CAT (p = 0.04), and GSH (p < 0.001) levels. NO2− levels were affected by drug dose (p = 0.038). TBARS (p < 0.001), O2− (p < 0.001), and SOD (p = 0.022) inversely correlated with PRL levels, suggesting PRL’s protective role against oxidative stress. The female sex association with higher PRL levels implies additional factors influencing PRL’s antioxidant role. Antipsychotic choice and dosage impact PRL and oxidative stress markers, necessitating further exploration.
Contrasting Disease Progression, Microglia Reactivity, Tolerance, and Resistance to Toxoplasma gondii Infection in Two Mouse Strains
Authors: Diniz Daniel G. , de Oliveira Jhonnathan H. P. , Guerreiro Luma C. F. , de Menezes Gabriel C. , de Assis Alexa C. L. , Duarte Tainá Q. , dos Santos Izabelly B. D. , Maciel Flávia D. , Soares Gabrielly L. da S. , Araújo Sanderson C. , Franco Felipe T. de C. , do Carmo Ediclei L. , Morais Rafaela dos A. B. , de Lima Camila M. , Brites Dora , Anthony Daniel C. , Diniz José A. P. , Diniz Cristovam W. P.
Published: June 2024

Our study investigated the innate immune response to Toxoplasma gondii infection by assessing microglial phenotypic changes and sickness behavior as inflammatory response markers post-ocular tachyzoite instillation. Disease progression in Swiss albino mice was compared with the previously documented outcomes in BALB/c mice using an identical ocular route and parasite burden (2 × 105 tachyzoites), with saline as the control. Contrary to expectations, the Swiss albino mice displayed rapid, lethal disease progression, marked by pronounced sickness behaviors and mortality within 11–12 days post-infection, while the survivors exhibited no apparent signs of infection. Comparative analysis revealed the T. gondii-infected BALB/c mice exhibited reduced avoidance of feline odors, while the infected Swiss albino mice showed enhanced avoidance responses. There was an important increase in microglial cells in the dentate gyrus molecular layer of the infected Swiss albino mice compared to the BALB/c mice and their respective controls. Hierarchical cluster and discriminant analyses identified three microglial morphological clusters, differentially affected by T. gondii infection across strains. The BALB/c mice exhibited increased microglial branching and complexity, while the Swiss albino mice showed reduced shrunken microglial arbors, diminishing their morphological complexity. These findings highlight strain-specific differences in disease progression and inflammatory regulation, indicating lineage-specific mechanisms in inflammatory responses, tolerance, and resistance. Understanding these elements is critical in devising control measures for toxoplasmosis.
Ischemia-Modified Albumin, Lactate, and Combination for Predicting Mortality in Patients with Septic Shock in the Emergency Department
Authors: Jin Bo-Yeong , Lee Sukyo , Kim Woosik , Park Jong-Hak , Cho Hanjin , Moon Sungwoo , Ahn Sejoong
Published: June 2024

Ischemia-modified albumin (IMA) is produced during ischemia and reactive oxygen species production. This study aimed to evaluate the association between IMA and mortality in a larger population and the prognostic value of the combination of IMA and lactate for predicting mortality in septic shock patients in the emergency department. This retrospective observational study included adult septic shock patients between October 2019 and December 2021. A multivariable Cox proportional hazards model was performed. IMA was significantly higher in the non-surviving group than in the surviving group (89.1 ± 7.2 vs. 83.8 ± 6.2 U/mL, p < 0.001). IMA was independently associated with 28-day mortality after adjustments (adjusted hazard ratio [aHR]: 1.075, 95% confidence interval [CI]: 1.016–1.138, p = 0.012). The area under the ROC curve (AUROC) of IMA was 0.712 (95% CI: 0.648–0.775, p < 0.001) and was comparable to that of lactate. The AUROC of the combination of IMA and lactate was 0.838 (95% CI: 0.786–0.889, p < 0.001). The group with both high lactate and high IMA levels showed an extremely high risk of mortality than other groups (86.1%; aHR 8.956, 95% CI 4.071–19.70, p < 0.001). The elevation of IMA was associated with mortality in septic shock patients. The combination of IMA and lactate can be a helpful tool for early risk stratification of septic shock patients.
Identification and Validation of JAM-A as a Novel Prognostic and Immune Factor in Human Tumors
Authors: Ren Tianyi , Zheng You , Liu Feichang , Liu Chenyu , Zhang Bo , Ren He , Gao Xinyue , Wei Yuexian , Sun Qiang , Huang Hongyan
Published: June 2024

Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R), is a transmembrane glycoprotein that is involved in various biological processes, including cancer initiation and progression. However, the functional characteristics and significance of JAM-A in pan-cancer remain unexplored. In this study, we used multiple databases to gain a comprehensive understanding of JAM-A in human cancers. JAM-A was widely expressed in various tissues, mainly located on the microtubules and cell junctions. Aberrant expression of JAM-A was detected in multiple cancers at both mRNA and protein levels, which can be correlated with poorer prognosis and may be attributed to genetic alterations and down-regulated DNA methylation. JAM-A expression was also associated with immune infiltration and may affect immunotherapy responses in several cancers. Functional enrichment analysis indicated that JAM-A participated in tight junction and cancer-related pathways. In vitro experiments verified that JAM-A knockdown suppressed the proliferation and migration abilities of breast cancer cells and liver cancer cells. Overall, our study suggests that JAM-A is a pan-cancer regulator and a potential biomarker for predicting prognosis and immune-therapeutic responses for different tumors.
Differential Impact of Tumor Endothelial Angiopoietin-2 and Podoplanin in Lymphatic Endothelial Cells on HCC Outcomes with Tyrosine Kinase Inhibitor Treatment According to Sex
Authors: Lasagni Simone , Critelli Rosina Maria , Milosa Fabiola , Saltini Dario , Schepis Filippo , Romanzi Adriana , Dituri Francesco , Serino Grazia , Di Marco Lorenza , Pivetti Alessandra , Scianò Filippo , Giannelli Gianluigi , Villa Erica
Published: June 2024

Hepatocellular carcinoma (HCC) is the second leading cause of cancer death worldwide. Curative treatments are available to a minority of patients, as HCC is often diagnosed at an advanced stage. For patients with unresectable and multifocal HCC, tyrosine kinase inhibitor drugs (TKIs) are the only potential treatment option. Despite extensive research, predictors of response to these therapies remain elusive. This study aimed to analyze the biological and histopathological characteristics of HCC patients treated with TKIs, focusing on angiogenesis and lymphangiogenesis. Immunohistochemistry quantified the expression of angiopoietin-2 (Ang2), lymphatic endothelial cells (LEC) podoplanin, and C-type Lectin Domain Family 2 (CLEC-2), key factors in neoangiogenesis and lymphangiogenesis. An increased expression of endothelial Ang2 and LEC podoplanin predicted a lower risk of metastasis. Female patients had significantly longer overall survival and survival on TKIs, associated with higher tumor expression of endothelial Ang2 and LEC podoplanin. Moreover, LEC podoplanin expression and a longer time on TKIs were independently correlated with improved survival on TKI therapy at Cox regression analysis. These findings suggest that endothelial Ang2 and LEC podoplanin could be potential biomarkers for predicting treatment outcomes and guiding therapeutic strategies in HCC patients treated with TKIs.
3D Spongin Scaffolds as Templates for Electro-Assisted Deposition of Selected Iron Oxides
Authors: Nowacki Krzysztof , Kubiak Anita , Nowicki Marek , Tsurkan Dmitry , Ehrlich Hermann , Jesionowski Teofil
Published: June 2024

The skeletons of marine sponges are ancient biocomposite structures in which mineral phases are formed on 3D organic matrices. In addition to calcium- and silicate-containing biominerals, iron ions play an active role in skeleton formation in some species of bath sponges in the marine environment, which is a result of the biocorrosion of the metal structures on which these sponges settle. The interaction between iron ions and biopolymer spongin has motivated the development of selected extreme biomimetics approaches with the aim of creating new functional composites to use in environmental remediation and as adsorbents for heavy metals. In this study, for the first time, microporous 3D spongin scaffolds isolated from the cultivated marine bath sponge Hippospongia communis were used for electro-assisted deposition of iron oxides such as goethite [α-FeO(OH)] and lepidocrocite [γ-FeO(OH)]. The obtained iron oxide phases were characterized with the use of scanning electron microscopy, FTIR, and X-ray diffraction. In addition, mechanisms of electro-assisted deposition of iron oxides on the surface of spongin, as a sustainable biomaterial, are proposed and discussed.
Path Planning of Unmanned Aerial Vehicles Based on an Improved Bio-Inspired Tuna Swarm Optimization Algorithm
Authors: Wang Qinyong , Xu Minghai , Hu Zhongyi
Published: June 2024

The Sine–Levy tuna swarm optimization (SLTSO) algorithm is a novel method based on the sine strategy and Levy flight guidance. It is presented as a solution to the shortcomings of the tuna swarm optimization (TSO) algorithm, which include its tendency to reach local optima and limited capacity to search worldwide. This algorithm updates locations using the Levy flight technique and greedy approach and generates initial solutions using an elite reverse learning process. Additionally, it offers an individual location optimization method called golden sine, which enhances the algorithm’s capacity to explore widely and steer clear of local optima. To plan UAV flight paths safely and effectively in complex obstacle environments, the SLTSO algorithm considers constraints such as geographic and airspace obstacles, along with performance metrics like flight environment, flight space, flight distance, angle, altitude, and threat levels. The effectiveness of the algorithm is verified by simulation and the creation of a path planning model. Experimental results show that the SLTSO algorithm displays faster convergence rates, better optimization precision, shorter and smoother paths, and concomitant reduction in energy usage. A drone can now map its route far more effectively thanks to these improvements. Consequently, the proposed SLTSO algorithm demonstrates both efficacy and superiority in UAV route planning applications.
Dentistry and Cranio Facial District: The Role of Biomimetics
Authors: Minervini Giuseppe
Published: June 2024

Biomimetics has emerged as a pivotal field, bridging fundamental research and practical applications [...]
Neural Effects of One’s Own Voice on Self-Talk for Emotion Regulation
Authors: Jo Hye-jeong , Park Chanmi , Lee Eunyoung , Lee Jee Hang , Kim Jinwoo , Han Sujin , Kim Joohan , Kim Eun Joo , Kim Eosu , Kim Jae-Jin
Published: June 2024

One’s own voice undergoes unique processing that distinguishes it from others’ voices, and thus listening to it may have a special neural basis for self-talk as an emotion regulation strategy. This study aimed to elucidate how neural effects of one’s own voice differ from those of others’ voices on the implementation of emotion regulation strategies. Twenty-one healthy adults were scanned using fMRI while listening to sentences synthesized in their own or others’ voices for self-affirmation and cognitive defusion, which were based on mental commitments to strengthen one’s positive aspects and imagining metaphoric actions to shake off negative aspects, respectively. The interaction effect between voice identity and strategy was observed in the superior temporal sulcus, middle temporal gyrus, and parahippocampal cortex, and activity in these regions showed that the uniqueness of one’s own voice is reflected more strongly for cognitive defusion than for self-affirmation. This interaction was also seen in the precuneus, suggesting intertwining of self-referential processing and episodic memory retrieval in self-affirmation with one’s own voice. These results imply that unique effects of one’s own voice may be expressed differently due to the degree of engagement of neural sharpening-related regions and self-referential networks depending on the type of emotion regulation.
New Perspectives of Deep Brain Stimulation Indications for Parkinson’s Disease: A Critical Review
Authors: Barbosa Renata Montes Garcia , Soares Miriam Carvalho , Portela Denise Maria Meneses Cury , Guimarães Thiago Gonçalves , Cury Rubens Gisbert
Published: June 2024

Deep Brain Stimulation (DBS) is an effective treatment option for patients with dopaminergic complications of Parkinson’s disease (PD) and drug-refractory PD tremor. However, DBS and its indications can be challenging, and they are not often debated in the medical community. Through a critical narrative review, the objective of this paper is to improve the comprehension of DBS indications and help to solve the puzzle that this process can be. Proper patient selection is the first step for a good surgical outcome. In this review, then, relevant considerations are discussed, involving PD genes, PD phenotypes, indications of early stages, non-motor symptoms, neuroimaging predictors, comorbidities, and age. Individualized approaches are encouraged, including clinical and radiological factors. Social support during the whole follow-up and expectations alignment are necessary through this process and are also debated.
Investigating Whether a Combination of Electro-Encephalography and Gene Expression Profiling Can Predict the Risk of Chronic Pain: A Protocol for an Observational Prospective Cohort Study
Authors: Sannes Ann-Christin , Ghani Usman , Niazi Imran Khan , Moberget Torgeir , Jonassen Rune , Haavik Heidi , Gjerstad Johannes
Published: June 2024

Despite most episodes of low back pain (LBP) being short-lasting, some transition into persistent long-lasting problems. Hence, the need for a deeper understanding of the physiological mechanisms of this is pertinent. Therefore, the aims of the present study are (1) to map pain-induced changes in brain activity and blood gene expression associated with persistent LBP, and (2) to explore whether these brain and gene expression signatures show promise as predictive biomarkers for the development of persistent LBP. The participants will be allocated into three different pain groups (no pain, mild short-lasting, or moderate long-term). One in-person visit, where two blood samples will be collected and sent for RNA sequencing, along with resting 64-channel electro-encephalography measurements before, during, and after a cold pressor test, will be conducted. Thereafter, follow-up questionnaires will be distributed at 2 weeks, 3 months, and 6 months. Recruitment will start during the second quarter of 2024, with expected completion by the last quarter of 2024. The results are expected to provide insight into the relationship between central nervous system activity, gene expression profiles, and LBP. If successful, this study has the potential to provide physiological indicators that are sensitive to the transition from mild, short-term LBP to more problematic, long-term LBP.
Fast Cable-Force Measurement for Large-Span Cable-Stayed Bridges Based on the Alignment Recognition Method and Smartphone-Captured Video
Authors: Lu Hongxia , Fu Wenwei , Chen Yi , Li Bingchun , Li Yang
Published: June 2024

The accurate measurement of cable force plays an important role in the structural form, structural behavior, and safety evaluation of large-span cable-stayed bridges. A fast cable-force estimation method is proposed based on the alignment recognition method and smartphone-captured video. This method can realize real-time, non-contact, and non-destructive force measurements. Videos of bridge cables were collected using a portable smartphone, and an alignment recognition method was employed to obtain the lateral displacement along the cable, followed by numerical differentiation of the lateral displacement of the cable to acquire the acceleration time history. Based on the fundamental dynamic equation of beam elements, a unified explicit formula for cable-force calculation considering service characteristics was established in the frequency method. Finally, the method was applied to the cable-force measurement during the operation and maintenance stage of the Gengcun Dou Bridge. The measured cable forces were compared with the values obtained from the on-site monitoring system. The results show that the proposed method can accurately identify the acceleration time history at different positions of the cable, and the corresponding frequency components are essentially consistent. The average relative deviation of the measured cable forces from the reference method is within 3%. The proposed cable-force measurement method is simple as regards equipment, convenient for operation, and high in efficiency, providing a new method for the measurement of cable forces in large-span cable-stayed bridges, which can offer reliable measured cable-force data for structural analysis during bridge operation and the maintenance stage.
Genomic Deletion of PFKFB3 Decreases In Vivo Tumorigenesis
Authors: Imbert-Fernandez Yoannis , Chang Simone M. , Lanceta Lilibeth , Sanders Nicole M. , Chesney Jason , Clem Brian F. , Telang Sucheta
Published: June 2024

Rapidly proliferative processes in mammalian tissues including tumorigenesis and embryogenesis rely on the glycolytic pathway for energy and biosynthetic precursors. The enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 (PFKFB3) plays an important regulatory role in glycolysis by activating the key rate-limiting glycolytic enzyme, 6-phosphofructo-1-kinase (PFK-1). We have previously determined that decreased PFKFB3 expression reduced glycolysis and growth in transformed cells in vitro and suppressed xenograft growth in vivo. In earlier studies, we created a constitutive knockout mouse to interrogate the function of PFKFB3 in vivo but failed to generate homozygous offspring due to the requirement for PFKFB3 for embryogenesis. We have now developed a novel transgenic mouse model that exhibits inducible homozygous pan-tissue Pfkfb3 gene deletion (Pfkfb3fl/fl). We have induced Pfkfb3 genomic deletion in these mice and found that it effectively decreased PFKFB3 expression and activity. To evaluate the functional consequences of Pfkfb3 deletion in vivo, we crossed Cre-bearing Pfkfb3fl/fl mice with oncogene-driven tumor models and found that Pfkfb3 deletion markedly decreased their glucose uptake and growth. In summary, our studies reveal a critical regulatory function for PFKFB3 in glycolysis and tumorigenesis in vivo and characterize an effective and powerful model for further investigation of its role in multiple biological processes.
Surgical and Oncologic Outcome following Sacrectomy for Primary Malignant Bone Tumors and Locally Recurrent Rectal Cancer
Authors: Weidlich Anne , Schaser Klaus-Dieter , Weitz Jürgen , Kirchberg Johanna , Fritzmann Johannes , Reeps Christian , Schwabe Philipp , Melcher Ingo , Disch Alexander , Dragu Adrian , Winkler Doreen , Mehnert Elisabeth , Fritzsche Hagen
Published: June 2024

Introduction: Bone sarcoma or direct pelvic carcinoma invasion of the sacrum represent indications for partial or total sacrectomy. The aim was to describe the oncosurgical management and complication profile and to analyze our own outcome results following sacrectomy. Methods: In a retrospective analysis, 27 patients (n = 8/10/9 sarcoma/chordoma/locally recurrent rectal cancer (LRRC)) were included. There was total sacrectomy in 9 (incl. combined L5 en bloc spondylectomy in 2), partial in 10 and hemisacrectomy in 8 patients. In 12 patients, resection was navigation-assisted. For reconstruction, an omentoplasty, VRAM-flap or spinopelvic fixation was performed in 20, 10 and 13 patients, respectively. Results: With a median follow-up (FU) of 15 months, the FU rate was 93%. R0-resection was seen in 81.5% (no significant difference using navigation), and 81.5% of patients suffered from one or more minor-to-moderate complications (especially wound-healing disorders/infection). The median overall survival was 70 months. Local recurrence occurred in 20%, while 44% developed metastases and five patients died of disease. Conclusions: Resection of sacral tumors is challenging and associated with a high complication profile. Interdisciplinary cooperation with visceral/vascular and plastic surgery is essential. In chordoma patients, systemic tumor control is favorable compared to LRRC and sarcomas. Navigation offers gain in intraoperative orientation, even if there currently seems to be no oncological benefit. Complete surgical resection offers long-term survival to patients undergoing sacrectomy for a variety of complex diseases.
Prognostic Role of CA-125 Elimination Rate Constant (KELIM) in Patients with Advanced Epithelial Ovarian Cancer Who Received PARP Inhibitors
Authors: Kim Ji Hyun , Kim Eun Taeg , Kim Se Ik , Park Eun Young , Park Min Young , Park Sang-Yoon , Lim Myong Cheol
Published: June 2024

Background: This multicenter retrospective study aimed to investigate the prognostic value of the CA-125 elimination rate constant K (KELIM) in EOC patients who received platinum-based chemotherapy followed by PARP inhibitors, in either upfront or interval treatment settings. Methods: Between July 2019 and November 2022, we identified stage III–IV EOC patients who underwent primary or interval cytoreductive surgery and received olaparib or niraparib. Individual KELIM values were assessed based on validated kinetics and classified into favorable and unfavorable cohorts. Results: In a study of 252 patients undergoing frontline maintenance therapy with olaparib or niraparib, favorable KELIM (≥1) scores were associated with a higher PFS benefit in the primary cytoreductive surgery (PCS) cohort (hazard ratio (HR) for disease progression or death 3.51, 95% confidence interval (CI); 1.37–8.97, p = 0.009). Additionally, within the interval cytoreductive surgery (ICS) cohort, a favorable KELIM score (≥1) significantly increased the likelihood of achieving complete resection following cytoreductive surgery, with 59.4% in the favorable KELIM group compared to 37.8% in those with unfavorable KELIM. Conclusions: A favorable KELIM score was associated with improved PFS in patients with advanced EOC undergoing PCS. Furthermore, in the ICS cohort, a favorable KELIM score increased the probability of complete cytoreduction.
Method for B Cell Receptor Enrichment in Malignant B Cells
Authors: Bhattacharyya Puja , Christopherson Richard I. , Skarratt Kristen K. , Fuller Stephen J.
Published: June 2024

B cells are central to the adaptive immune response and provide long-lasting immunity after infection. B cell activation is mediated by the surface membrane-bound B cell receptor (BCR) following recognition of a specific antigen. The BCR has been challenging to analyse using mass spectrometry (MS) due to the difficulty of isolating and enriching this membrane-bound protein complex. There are approximately 120,000 BCRs on the B cell surface; however, depending on the B cell activation state, there may be hundreds-of-millions to billions of proteins in a B cell. Consequently, advanced proteomic techniques such as MS workflows that use purified proteins to yield structural and protein-interaction information have not been published for the BCR complex. This paper describes a method for enriching the BCR complex that is MS-compatible. The method involves a Protein G pull down on agarose beads using an intermediary antibody to each of the BCR complex subcomponents (CD79a, CD79b, and membrane immunoglobulin). The enrichment process is shown to pull down the entire BCR complex and has the advantage of being readily compatible with further proteomic study including MS analysis. Using intermediary antibodies has the potential to enrich all isotypes of the BCR, unlike previous methods described in the literature that use protein G-coated beads to directly pull down the membrane IgG (mIgG) but cannot be used for other mIg isotypes.
Uniportal Video-Assisted Thoracoscopic Segmentectomy for Early-Stage Non-Small Cell Lung Cancer: Overview, Indications, and Techniques
Authors: Watanabe Takuya , Tanahashi Masayuki , Suzuki Eriko , Yoshii Naoko , Kohama Takuya , Iguchi Kensuke , Endo Takumi
Published: June 2024

Twenty years have passed since uniportal video-assisted thoracoscopic surgery (VATS) was first reported. Several reports have already proven the minimal invasiveness of uniportal VATS. In addition, two large clinical trials recently demonstrated the benefits of segmentectomy for small peripheral early-stage non-small cell lung cancer. Uniportal VATS segmentectomy is considered the most beneficial minimally invasive surgery for patients with early-stage lung cancer. However, a high level of skill and experience are required to achieve this goal. Only a few reports have discussed specific techniques, particularly for complex segmentectomies. In this Special Issue, we reviewed previous reports on uniportal VATS segmentectomy regarding the indications, instrument selection, marking of the tumor location, methods of intersegmental plane identification, and lymph node dissection, including our own techniques with video content.
Ni-Ag Catalysts for Hydrogen Production through Dry Reforming of Methane: Characterization and Performance Evaluation
Authors: Henni Hayat , Benrabaa Rafik , Roussel Pascal , Löfberg Axel
Published: June 2024

To investigate the influence of Ag and the loading of Ni species, Ni-Ag type catalysts were synthesized with varying Ni/Ag ratios (1, 1.5 and 2) using the coprecipitation method. The catalysts were extensively characterized using various techniques such as TG-DSC-SM, XRD, ICP, BET, SEM-EDX and TPR and subsequently tested in the CH4/CO2 reaction without any pretreatment. Regardless of the ratio employed, a phase mixture containing NiO and Ag was observed after calcination under air between 600 °C and 1200 °C. SEM analysis confirmed the presence of a close interface between Ag and NiO. The specific surface area was found to be significantly higher for the catalyst with lower Ni content (R = 1). TPR analysis demonstrated that the inclusion of Ag facilitated the reduction of Ni at lower temperatures. XRD analyses of the spent catalyst confirmed catalyst reduction during the reaction. Among the samples, a catalyst with Ni/Ag = 1 exhibited superior catalytic activity without any pretreatment under a reduction atmosphere, in which case the conversions of methane and CO2 at 650 °C amounted to 38 and 45 mol%, respectively, with H2/CO = 0.7 and 71 mol% of H2. The presence of Ag species enhances the stability of the Ni catalyst and improves catalytic performance in the dry reforming of methane.
Hydroprocessing of Gasoline on Modified Alumina Catalysts
Authors: Tuktin Balga , Saidilda Galymzhan , Nurzhanova Saule , Ongarbayev Yerdos
Published: June 2024

The hydroprocessing of gasoline on modified alumina catalysts makes it possible to obtain high-octane products. The implementation and development of the process have largely become possible due to the development of modified alumina catalysts that do not contain noble metals and exhibit special catalytic properties. This article discusses topical issues of petrochemistry, namely the creation of catalysts with improved characteristics for the production of high-octane gasoline with low sulfur content. New catalytic systems based on alumina and other carriers modified with transition metals, lanthanum and phosphorus were synthesized. By physico-chemical methods of analysis TPD of ammonia, TEM and XRD, we studied the acid–base and structural characteristics of the developed catalysts. The activity of the developed catalysts in the studied process of hydrotreating gasoline fractions depends on the structure and condition of the active centers. The process of hydrotreating straight-run gasoline in the presence of synthesized catalysts was carried out on a laboratory flow unit. It was shown that, during the hydrotreating of straight-run gasoline on the NiO-MoO3-La-P-HZSM-HY-Al2O3 catalyst, the octane number in the final product increased to 88.6, and the sulfur content decreased from 0.0088 to 0.001%. It was found that the minimum sulfur content in the gasoline hydrotreating product of 0.0005% was achieved on the catalyst CoO-WO3-La-P-HZSM-HY-Al2O3, which is significantly lower than for other studied catalytic systems. The obtained results of the sulfur content in the hydrotreating products fully comply with the Euro-5 standard. Thus, the efficiency of hydrotreating the gasoline fractions studied in this work was mainly determined by the nature of the carriers and modifiers used for the synthesis of catalysts and the technological parameters of the process. The synthesized catalysts showed high activity and selectivity, resulting in high-octane gasoline with a low sulfur content that meets international quality standards.
Comprehensive Review of Uterine Leiomyosarcoma: Pathogenesis, Diagnosis, Prognosis, and Targeted Therapy
Authors: Yang Qiwei , Madueke-Laveaux Obianuju Sandra , Cun Han , Wlodarczyk Marta , Garcia Natalia , Carvalho Katia Candido , Al-Hendy Ayman
Published: June 2024

Uterine leiomyosarcoma (uLMS) is the most common subtype of uterine sarcomas. They have a poor prognosis with high rates of recurrence and metastasis. The five-year survival for uLMS patients is between 25 and 76%, with survival rates approaching 10–15% for patients with metastatic disease at the initial diagnosis. Accumulating evidence suggests that several biological pathways are involved in uLMS pathogenesis. Notably, drugs that block abnormal functions of these pathways remarkably improve survival in uLMS patients. However, due to chemotherapy resistance, there remains a need for novel drugs that can target these pathways effectively. In this review article, we provide an overview of the recent progress in ascertaining the biological functions and regulatory mechanisms in uLMS from the perspective of aberrant biological pathways, including DNA repair, immune checkpoint blockade, protein kinase and intracellular signaling pathways, and the hedgehog pathway. We review the emerging role of epigenetics and epitranscriptome in the pathogenesis of uLMS. In addition, we discuss serum markers, artificial intelligence (AI) combined with machine learning, shear wave elastography, current management and medical treatment options, and ongoing clinical trials for patients with uLMS. Comprehensive, integrated, and deeper insights into the pathobiology and underlying molecular mechanisms of uLMS will help develop novel strategies to treat patients with this aggressive tumor.
MLIP and Its Potential Influence on Key Oncogenic Pathways
Authors: Hamwi Mahmoud N. , Elsayed Engy , Dabash Hanan , Abuawad Amani , Aweer Noor A. , Al Zeir Faissal , Pedersen Shona , Al-Mansoori Layla , Burgon Patrick G.
Published: June 2024

Muscle-enriched A-type lamin-interacting protein (MLIP) is an emerging protein involved in cellular homeostasis and stress adaptation. Eukaryotic cells regulate various cellular processes, including metabolism, DNA repair, and cell cycle progression, to maintain cellular homeostasis. Disruptions in this homeostasis can lead to diseases such as cancer, characterized by uncontrolled cell growth and division. This review aims to explore for the first time the unique role MLIP may play in cancer development and progression, given its interactions with the PI3K/Akt/mTOR pathway, p53, MAPK9, and FOXO transcription factors, all critical regulators of cellular homeostasis and tumor suppression. We discuss the current understanding of MLIP’s involvement in pro-survival pathways and its potential implications in cancer cells’ metabolic remodeling and dysregulated homeostasis. Additionally, we examine the potential of MLIP as a novel therapeutic target for cancer treatment. This review aims to shed light on MLIP’s potential impact on cancer biology and contribute to developing innovative therapeutic strategies.
Indigenous Strategies for Managing Bush Encroachment in Rural Areas of South Africa
Authors: Baloyi Tshidi Mokgatsane , Maphanga Thabang , Madonsela Benett Siyabonga , Mongwe Qolani Golden , Malakane Karabo Concelia , Grangxabe Xolisiwe Sinalo , Gqomfa Babalwa
Published: June 2024

The integration of indigenous knowledge systems into the discussion of bush encroachment management is of paramount importance. Indigenous knowledge and formal monitoring may be mutually beneficial, and using both approaches can improve natural resource management. Savannah rangeland landscapes hold deep cultural and spiritual significance for indigenous communities, and their perceptions can provide valuable insights into creating more effective, community-driven conservation initiatives. This study was aimed at filling the existing knowledge and research gap on bush encroachment control by focusing on the integration of indigenous knowledge systems. To achieve this, the current research included three distinct non-probability sampling strategies: (1) Purposive, (2) Snowball, (3) Convenience sampling methods. The results showed that 90.3% of the participants indicated that the main encroaching species of concern was sickle bush (Dichrostachys cinerea) and it is therefore perceived as a problem in the rangeland. The majority of farmer respondents indicated that they cut the encroacher plant down, uproot all root systems, then burn the remaining roots. This is reported to be a more effective way of managing sickle bush as an encroacher plant. Both genders generally believe in the efficacy of these systems, with variations in levels of agreement. However, a gender disparity emerges in opinions on incorporating communal-based approaches, emphasizing the need to consider gender perspectives in environmental management initiatives. Therefore, considering this, the study concludes that a holistic approach, integrating both formal and informal knowledge systems, may be crucial for sustainable and effective management strategies. Given that, recognizing the diversity of perspectives within the community, particularly regarding gender differences, is essential for developing inclusive and community-driven environmental management initiatives.