bluuebunny/crossref_metadata_2025 huggingface dataset:
Decentralized control for interconnected semi-markovian jump systems with partially accessible transition rates: a dynamic memory event-triggered mechanism
Authors: Tan Yushun , Cheng Xiaoming , Li Xinrui , Bai Jie , Li Jinliang
Published: May 2023

This paper investigates the issue of decentralized control for interconnected semi-Markovian systems with partially accessible transition rates (TRs). Firstly, a dynamic system model with a memory event-triggered mechanism (METM) is designed, which can effectively improve the fault tolerance of the event-triggering mechanism by employing the historical trigger data. Then a state feedback control model with dynamic METM is constructed, in which the semi-Markovian parameters with completely unknown and partially known transition probabilities are considered. Some sufficient conditions that insure the stochastic stability of the interconnected semi-Markovian systems can be obtained by utilizing the Lyapunov function and suitable model transformations method. Meanwhile, the parameters and the controller gain matrices of dynamic METM are also solved simultaneously by applying the linear matrix inequalities (LMIs). Finally, a simulation example is given to verify the effectiveness of the proposed method.
Protective Effect of Amaranthus cruentus Seed Oil on the UVA Radiation-Induced Apoptosis in Human Skin Fibroblasts
Authors: Wolosik Katarzyna , Chalecka Magda , Palka Jerzy , Surazynski Arkadiusz
Published: May 2023

UVA radiation inducing oxidative stress is harmful for the skin cells. The most sensitive are dermal fibroblasts. Exposure of the cells to prolonged UVA radiation induces apoptosis. Although, skin cells have a number of defense mechanisms that protect them against UV-induced oxidative stress, they are insufficient during long-lasting UV exposure. Therefore, there is a need for effective skin protection compounds with cytoprotective and antioxidant properties. One of their sources is Amaranthus cruentus L. seed oil, rich in unsaturated fatty acids, squalene, vitamin E derivatives, and phytosterols. The aim of this study was to evaluate whether Amaranthus cruentus seed oil evokes protective effect on the apoptosis stimulated by UVA radiation in human skin fibroblasts. UVA radiation at an applied dose of 10 J/cm2 caused a significant reduction in the survival of human skin fibroblasts and directed them into apoptosis pathway. Increased expression of p53, caspase 3, and caspase 9 and PARP proteins in UVA-treated fibroblasts suggests the intrinsic mechanism of apoptosis. The application of Amaranthus cruentus seed oil at 0.1% and 0.15% concentrations to UVA-treated cells decreased the expression of these proteins, which was accompanied by increased cell survival. Similarly, the UVA-dependent decrease in expression of p-Akt and mTOR proteins was restored, under the effect of studied oil. The molecular mechanism of this phenomenon is related to the stimulation of antioxidant processes through activation of Nrf2. This suggests that Amaranthus cruentus seed oil stimulate the antioxidant system in fibroblast cells and prevent the effects of UVA-induced oxidative stress and may find application in pharmacy and cosmetology as a sun- protective substance.
Phylogeny Analysis and Functional Characterizatiion of CYP76AHs from Salvia
Authors: Zhao Zhenyu , Yang Dongfeng , Guo Juan , Liu Xiuyu , Ma Ying , Huang Luqi
Published: May 2023

Salvia is a large genus in traditional Chinese medicine with hundreds of species. Tanshinones are a highly representative class of exclusive compounds found in the Salvia genus that exhibit significant biological activity. Tanshinone components have been identified in 16 species of the Salvia genus. The CYP76AH subfamily (P450) is crucial for the synthesis of tanshinone due to its catalytic generation of polyhydroxy structures. 420CYP76AH genes were obtained, clear clustering relationships. In this study 15 CYP76AH genes from 10 Salvia species were cloned and studied from the perspectives of evolution and catalytic efficiency. Structure-function relationship revealed several conserved residues which might be related to the function of CYP76AHs. Amino acids (97I, 116V, and 290T) are conserved in CYP76AH1 group, while amino acids (117G, 208S, 237Y, and 479F) are CYP76AH3 group protein sequence characteristics with unique enzyme function.This research holds significant implications for the classification of the Salvia genus and provide efficient catalytic elements for the synthetic biology production of tanshinones.Keywords: Salvia; CYP76AH; P450; biosynthesis; functional gene
An Improved Design of the MultiCal on-site Calibration Device for Industrial Robots
Authors: Wan Ziwei , Zhou Chunlin , Lin Zhaohui , Yan Huapeng , Tang Weixi , Wang Zhen , Wu Jun
Published: May 2023

MultiCal is an affordable, high-precision measuring device designed for the on-site calibration of industrial robots. Its design features a long measuring rod with a spherical tip that is attached to the robot. By restricting the rod’s tip to multiple fixed points under different rod orientations, the relative positions of these points are accurately measured beforehand. A common issue with MultiCal is the gravity deformation of the long measuring rod, which introduces measurement errors into the system. This problem becomes especially serious when calibrating large robots, as the length of the measuring rod needs to be increased to enable the robot to move in a sufficient space. To address this issue, we propose two improvements in this paper. Firstly, we suggest the use of a new design of the measuring rod that is lightweight yet has high rigidity. Secondly, we propose a deformation compensation algorithm. Experimental results have shown that the new measuring rod improves calibration accuracy by 20% to 39%, while by using the deformation compensation algorithm, the accuracy increases by 6% to 16%. In the best configuration, the calibration accuracy is similar to that of a measuring arm with a laser scanner, producing an average positioning error of 0.274 mm and a maximum positioning error of 0.838 mm. The improved design is cost-affordable, robust, and has sufficient accuracy, making MultiCal a more reliable tool for industrial robot calibration.
Towards quantum gravity
Authors: Kruglov Sergey
Published: May 2023

We analyze different approaches to quantum gravity. It is stressed that nonperturbative methods to quantise gravity and the usage of diffeomorphism-invariant variables are very important. We pay attention on the Wheeler--DeWitt equation in the framework of canonical quantum gravity. The Wheeler--DeWitt equation is presented in the first order formalism with the hope that this form can solve some problems such as singularities and the ordering. Also, there is a problem of defining the time.
Molecular Profile of Canine Hemangiosarcoma and Potential Novel Therapeutic Targets
Authors: Pimentel Pedro Antônio Bronhara , Giuliano Antonio , Bęczkowski Paweł M. , Horta Rodrigo Dos Santos
Published: May 2023

Canine hemangiosarcoma (HSA) is a relatively common neoplasia, occurring mainly in the skin, spleen, liver and right atrium. Despite the numerous studies investigating the treatment of canine HSA, no significant improvement in survival has been achieved in the last 20 years. Advancements in genetic and molecular profiling presented molecular similarities of canine HSA with human angiosarcoma. It could therefore serve as a valuable model for investigating new and more effective treatments in people and dogs. The most common genetic abnormalities in canine HSA have been found in the phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) and neuroblastoma RAS viral oncogene homolog (NRAS) pathways. Mutations are also found in tumor protein p53 (TP53), phosphatase and tensin homolog (PTEN) and cyclin dependent kinase inhibitor 2A (CDKN2A). Known abnormal protein expression could be exploited to trial new target treatments that could be beneficial for both canine and human patients. Despite the high expression of vascular endothelial growth factor (VEGF) and its receptor (VEGFR), no correlation with overall survival time has ever been found. In this review, we explore the most recent developments in molecular profiling in canine HSA and discuss their possible applications in the prognosis and treatment of this fatal disease.
Evolution Game Analysis of Chemical Risk Supervision Based on Different Modes: Special Rectification and Normal Regulation
Authors: Wang Xunqing , Huang Xinpeng , Zhou Hang , Zhang Nan , Sun Xiaoyu
Published: May 2023

Chemical safety is related to public health, safety and environmental concerns, and the dangerous chemicals safety problem is becoming the one that people commonly pay attention to. Strengthening chemical safety supervision not only involves safe production, but also is an important link to maintain social safety. Most related studies focus on chemical safety under normal regulation, this paper addresses the perspective of ‘special rectification’ and ‘normal regulation’, and establishes an evolutionary game model between chemical enterprises and government supervision departments under different supervision modes. Based on the evolutionary game theory, this paper studies the evolution process of the two game players' strategy choices, and compares and analyzes the evolution, stability and equilibrium between the chemical safety and the behavior strategies of government regulatory departments. The model is effectively analyzed based on numerical simulation, and the results show that: Under the ‘special rectification’ mode, the strategic choice of chemical enterprises engaging in safety without investment depends on the difference between the benefits and costs of safety without investment; In the ‘normal regulation’ mode, the choice of its safety non-investment strategy depends on the difference between the cost of engaging in safety investment and the cost of safety non-investment; Increasing the government's punishment will encourage chemical enterprises to take safety investment behavior under the two supervision modes. Increasing the punishment has a significant impact on the safety investment behavior of chemical enterprises under the ‘normal regulation’ mode, but it has no significant impact on the behavior of chemical enterprises under the ‘special rectification’ mode. At the same time, increasing the punishment will inhibit the strict supervision behavior of the government. The research conclusion provides important decision support for government departments to effectively supervise the safety production of chemical enterprises.
Research on the Impact of Customer Predictive Ability on Sustainable Innovation in Customized Enterprises
Authors: Shen Huayan , Bi Kexin , Ou Zhiyong , Gao Yu
Published: May 2023

With the disruption of digital technologies, customers have emerged as co-producers in order to reduce costs and enhance productivity. Customer-driven business logic recognizes customer capabilities and sustainable innovation as key factors in the performance of customized manufacturing enterprises. The potential relationship between customers and producers has become a new area of inquiry. Existing research rarely delves into the impact of customer predictive ability on the process of customized production activities, particularly in relation to sustainable innovation, which remains inadequately characterized. Ships serve as a typical representation of customized enterprises. This study explores the underlying drivers of sustainable innovation through customer demand orientation by examining 20-year historical patterns in the global shipping and shipbuilding markets, considering the environmental dependency and temporal correlation characteristics of the shipbuilding market (producers) and the shipping market (consumers). By employing time series and panel data in a machine learning algorithm, specifically the random forest model, the study reveals a strong and statistically significant correlation between new ship deliveries and the Baltic Dry Index (BDI), with larger value ships having a more pronounced impact on the consumer market. The correlation analysis confirms that these two variables, in combination, can comprehensively reflect customer predictive ability and serve as crucial decision criteria for customer investment in new ship production. Furthermore, based on principal component analysis of customer predictive ability and ship innovation levels, as well as Granger causality tests, the study demonstrates that customer predictive ability is a Granger cause of sustainable innovation in customized production. Customer predictive ability influences sustainable innovation in customized enterprises to varying degrees. This research provides valuable insights for shipbuilding companies in terms of engaging in sustainable innovation in international markets and understanding the value of international market customers.
In-vivo Biomechanics and Corneal Structure Alterations Induced by Acoustic Waves
Authors: Ávila Francisco J. , Marcellán Maria Concepción , Remón Laura
Published: May 2023

The cornea is the optical window to the brain. Its optical and structural properties are responsible for optical transparency and vision. The shape, elasticity, rigidity or stiffness are given by its biomechanical properties, whose stability results in ocular integrity and intraocular pressure dynamics. Here, we report in vivo observation of structural changes and biomechanical alterations of the human cornea induced by acoustic wave pressure within the frequency range of 50-350 Hz and 90 dB of sound pressure level. Central corneal thickness (CCT) and eccentricity (e2) were measured using Scheimpflug imaging and biomechanical properties [corneal hysteresis (CH) and intraocular pressure (IOP] were assessed with air-puff tonometry in 6 young healthy subjects. At the specific 150 Hz acoustic frequency, the variation in CCT and e2 were of 0.058 and 7.33 µm, respectively. Biomechanical alterations were also observed in both IOP (decrease of 3.60 mmHg) and CH that showed an increase of 0.40 mmHg.
Strategi Bersaing Mewujudkan Madrasah Unggul (Studi di Mts Negeri 2 Ponorogo)
Authors: Kusuma Intan Widya , S Maryam Yusuf
Published: May 2023

Peranan kepala sekolah atau madrasah menjadi tokoh penting dalam setiap hal yang berhubungan dengan suksesnya pendidikan di lembaganya. Mewujudkan inovasi strategis didukung dengan pemikiran terbuka pada perubahan apapun, artinya sebagai pemimpin yang visioner harus mampu menemukan cara baru di luar kebiasaan umum. Dalam bidang apapun, orang yang sudah terbiasa melakukan sesuatu mengikuti kebiasaan umum, sudah pasti cara yang digunakan adalah cara biasa, sehingga bisa jadi mati layu sebelum berkembang. Latar belakang adanya program yang ada di MTsN 2 Ponorogo berdasarkan UU No. 22 Tahun 1999 tentang Pemerintahan Daerah dan diterapkannya manajemen berbasis sekolah (MBS) dalam kebijakan dan reformasi pendidikan, dengan ini menjadikan perubahan paradigma pendidikan dari sentralistik ke desentralisasi, dari pola penyelenggaraan pendidikan yang bersifat birokratis, hirarkis, menuju demokratis. Mengacu pada aturan tersebut, maka MTsN 2 Ponorogo mengupayakan berbagai perubahan pendidikan tradisional menuju pendidikan internasional. Penelitian ini bertujuan untuk mengidentifikasi program-program unggulan sebagai perwujudan madrasah yang kompetitif. Penelitian ini menggunakan pendekatan kualitatif dan desain penelitian studi kasus. Adapun hasil penelitian menunjukkan keberhasilan MTsN 2 Ponorogo dalam membina bakat dan minat siswa menjadikannya sebagai madrasah unggul yang diperhitungkan masyarakat, dilihat dari: a) Pencapaian skor akreditasi lembaga dengan predikat A; b) Perbaikan kualitas sikap siswa melalui kegiatan ma’had dan pembiasaan perilaku baik sehari-hari; c) Meningkatnya prestasi siswa, secara akademik maupun non akademik, selain itu kualitas lulusannya dapat diperhitungkan; d) Pengadaan program unggulan; dan e) Inovasi kurikulum
Integrasi Program Adiwiyata Dengan Dimensi Manajerial Sekolah Di SMPN 1 Lembeyan Magetan
Authors: Sulastri  , AB. Musyafa’ Fathoni
Published: May 2023

Abstrak

Penelitian ini dilatarbelakangi oleh tingginya sikap kurang peduli lingkungan di kalangan masyarakat terutama kaum remaja. Dalam hal ini peran sekolah sangat dibutuhkan untuk membentuk karakter peduli lingkungan siswa, untuk menyikapi hal tersebut sekolah mengimplementasikan program adiwiyata. Namun dalam menerapkan program tersebut sekolah harus mampu mengintegrasikan program adiwiyata dengan seluruh aspek manajerial sekolah, agar semua dapat terlaksana dengan lancar. Penelitian ini termasuk jenis penelitian lapangan dengan pendekatan kualitatif. Teknik pengumpulan data melalui metode wawancara, observasi serta dokumentasi. Analisis data yang digunakan meliputi pengumpulan data, penyajian data, reduksi data, dan penarikan kesimpulan. Tujuan penelitian ini untuk menjelaskan latar belakang sekolah menerapkan adiwiyata, dan menguraikan implikasi adiwiyata terhadap aspek manajerial sekolah, serta mengetahui cara sekolah dalam menyinkronisasikan program adiwiyata dengan aspek manajerial sekolah. Temuan yang diperoleh, dalam melaksanakan program adiwiyata dilatarbelakangi oleh adanya instruksi dari dinas pendidikan, maka dari itu adiwiyata di SMPN 1 Lembeyan bisa dikatakan sebagai bentuk kebijakan top down. Penerapan program adiwiyata berimplikasi pada dimensi manajerial sekolah yang bersifat penambahan, perubahan dan pengurangan. Upaya yang dilakukan sekolah dalam menyinkronisasi program adiwiyata dengan aspek manajerial sekolah dilakukan dengan beberapa bentuk di antaranya adalah dengan melakukan akulturasi, penempatan, identifikasi dan interaksi.
Manajemen Pembelajaran Tahfizul Qur’an Di MTsN 3 Ponorogo Era Pandemi Covid-19
Authors: Sholikin Muhammad sholikin , Dhinuk Puspita Kirana
Published: May 2023

Menghafal Al-Qur’an bukanlah hal yang sederhana dan tidak bisa dilakukan kebanyakan orang tanpa meluangkan waktu khusus. MTsN 3 Ponorogo merupakan lembaga pendidikan yang menerapkan pembelajaran Tahfizul Qur’an. Penelitian ini bertujuan untuk menjelaskan: (1) Perencanaan Pembelajaran tahfiz di MTsN 3 Ponorogo. (2) Pengorganisasian Pembelajaran tahfiz di MTsN 3 Ponorogo (3) Pelaksanaan Pembelajaran tahfiz di MTsN 3 Ponorogo. (4) Evaluasi Pembelajaran tahfiz di MTsN 3 Ponorogo. Penelitian ini merupakan penelitian lapangan yang menggunakan pendekatan deskriptif kualitatif dengan jenis penelitian studi kasus. Pengumpulan data penelitian ini dilakukan melalui observasi, wawancara mendalam, dan dokumentasi. Berdasarkan proses pengumpulan data dan analisis data penelitian ini menghasilkan temuan bahwa dalam Perencanaan Manajemen Pembelajaran Tahfizul Qur’an di MTsN 3 Ponorogo yakni komponen Standar Kompetensi atau Kompetensi Inti belum dicatumkan, pengorganisasian pembelajaran Tahfizul Qur’an dalam memberikan fasilitas kelengkapan pembelajaran, kedudukan guru untuk menentukan dan mendesain dalam pembelajaran sesuai dengan Konsekuensi dari Surat Keputusan Kepala Madrasah, pelaksanaan pembelajaran Tahfiẓul Qur’an dilakukan secara daring dengan memanfaatkan akses internet, menggunakan media handphone melalui pesan group whatsApp, Hasil evaluasi program pembelajaran Tahfiẓ Al-Qur’an terkait Perencanaan, organisasi, pelaksanaan dan evaluasi ada yang terlaksana dengan baik ada juga yang belum dan akan melakukan perbaikan dan penyempurnaan. Dan Pengunaan internet melalui pesan group whatsApp di MTsN 3 Ponorogo juga memiliki dampak positif dan negatif bagi guru maupun siswa.
إبستمولوجيا نظرية تضافر القرائن لتمام حسان
Authors: Imron Rosidi  , Zahrul Fata
Published: May 2023

الـملخص

إن وجود النظرية النقدية المستوحاة من كارل ماركس موجه في الواقع لاستعادة القيم الإنسانية والكرامة ككل. لذلك، فهو أكثر تحررًا. كملاحظة حاسمة، على الرغم من وجود نظرية نقدية في روح ماركس، إلا أن هذا لا يعني أن يتبنى نفسه وأن يكون عقائديًا. وبسبب ذلك، تُعرف هذه النظرية النقدية عمومًا باسم الماركسية الجديدة. ومع ذلك، في الواقع، كانت هذه النظرية النقدية مقتبسة من أساس الماركسية وروحها، أي تنظيم البشر المتحررين من قيود خلقهم. يهدف هذا المقال إلى تقديم النظرية النقدية لمدرسة فرانكفورت وتأثيرها على فكر اللغوي العربي تمام حسان لفهم الأنساب العلمية والمعرفية من خلال إحدى نظرياته، وهي "تضافر القرائن". هذا البحث عبارة عن دراسة مكتبية باستخدام تحليل الخطاب والنص بطريقة نظرية التأريض. ومن ثمّ استخدم نموذج ترميز شتراوس وكوربين بطريقة تحليل المحتوى من فيليب ما يرنج. ومصادر البيانات الأولية لـهذه الدراسة هي كتب تمام حسن. وتشير نتائج هذه الدراسة إلى وجود تأثير للنظرية النقدية لمدرسة فرانكفورت على فكر تمام حسن في نظرية تضافر القرائن بمؤشرات التفكير التاريخي والكلية، ونقد المقولات العلمية، ولا يفصل بين النظرية والتطبيق. وفي الوقت نفسه، فإن النقاط الحاسمة لمدرسة فرانكفورت الفكرية الواردة في تفكير تمام حسن هي التحرر (emancipatory) والتنوير (aufklarung) والـهيمنة المضادة في ظاهرة المجتمع الاجتماعي في محاولة لتوفير الوعي.
Implementation of a New Proprietary Vortex Fluid Sucker Rod Pump System to Improve Production by Enhancing Flow Dynamics
Authors: Coyes Corbin , Williams Benny , Jensen Camille , Conner Mike , Link Bradley , Saponja Jeff , Quinn Jordy
Published: May 2023

Conventional ball valve systems and insert-guided cages compromise performance due to gas interference, solids accumulation, and ball vibration that shortens the life and efficiency of conventional traveling and standing valve cages. The analysis of the flow profile around a common ball valve resulted in the design of a new proprietary pump system that maximizes fluid flow, creating a vortex profile that extends service life while increasing production.

The proprietary vortex fluid pump system was compared against conventional inserts during in-house testing and in a laboratory flow loop. Minimum to maximum flow rates were digitally measured to calculate the pressure drop at each flow rate with and without injecting gas. The transparent flow loop tubing allowed a visual qualitative assessment of fluid flow.

During laboratory testing, conventional inserts measured high ball vibration with excessive pressure drop. The proprietary vortex fluid pump system had no ball vibration, with a significant pressure drop decrease, and gas remained entrained as it cycled through a vortex flow. The results from laboratory testing showed an average 40–46% pressure drop decrease compared to conventional inserts.

Laboratory data were confirmed in numerous field applications as well as four case studies from four different fields for four separate operators. The vortex fluid pump system showed greater pump efficiencies and pump longevity. After installation of the system, cumulative results were combined to show an average 46% production increase over 485 wells in North America in 1 year.

The proprietary vortex fluid pump system decreases erratic velocity profile and reduces vibration in the valve system resulting in improved efficiency and reliability of sucker rod pumps. The design optimizes flow dynamics enabling the ball to remain stationary, allowing smaller and lighter balls and increasing the cross-sectional flow area in the most restrictive pump section. The design reduces solids accumulation, lessens cage wear, improves pump efficiency, and increases production. The vortex fluid pump system replaces all conventional valve systems.
pH Responsiveness of Microwave Polymer-Type Modified Magnetic Nanoparticles for Synergistic Emulsion-Breaking Effect on Thick Oil
Authors: Sun Nana , Shen Lisha , Sun Huina , Hu Jianbo
Published: May 2023

In this study, a polymeric magnetic emulsion-breaking compound of ferric tetroxide and cationic polyacrylamide (Fe3O4@CPAM) was designed and synthesized, and its functional groups, chemical bonding, magnetic properties, and thermal stability were characterized. Finally, the pH-responsive behavior of the microwave-modified magnetic nanoparticles (MNPs) in terms of their demulsification effect on thick oil-in-water emulsions was investigated using the morphology and distribution of oil droplets, zeta potential, and contact angle of the MNPs. The results showed that with increasing pH, the water separation of the emulsion initially decreased and then increased, and the best emulsion-breaking effect was achieved at pH 3. The highest water partition of the emulsion was 64.39% at a concentration of 175 mg/L for Fe3O4 MNPs alone before modification. Under the same conditions, the water separation rate of the emulsion for Fe3O4@CPAM was 78.95%, indicating that, compared with the conventional demulsification method, microwaves can promote oil-water separation. The highest water separation rate of MNPs before microwave modification was 85% at pH 3, whereas the water separation rate of MNPs after microwave modification reached 94.70% under the same conditions. This proves that microwaves and modified MNPs have a synergistic emulsion-breaking effect, mainly because the combination of microwaves and polymer modification enhances the hydrophobicity and neutralizes the charge of the MNPs, thus improving the emulsion-breaking efficiency.
Data Assimilation of Production and Multiple 4D Seismic Acquisitions in a Deepwater Field Using Ensemble Smoother with Multiple Data Assimilation
Authors: Rossi Rosa Daiane , Schiozer Denis José , Davolio Alessandra
Published: May 2023

In recent years, time-lapse (4D) seismic (4DS) data have been widely used for reservoir monitoring to provide relevant information on dynamic changes occurring during production. In complex reservoirs, multiple seismic monitor surveys are usually available. Updating reservoir models with these data can be very beneficial to improve the field’s management. In the quantitative integration of 4DS data into the data assimilation (DA) process, it is crucial to define how to deal with more than one seismic monitor. In this work, we continue a series of investigations about seismic DA procedures and expand on them by analyzing ways to assimilate more than one seismic monitor. More specifically, we evaluate different ways of using production data and two monitor surveys (M3 and M5) to calibrate the dynamic models of a real Brazilian reservoir using the ensemble smoother with multiple data assimilation (ES-MDA) method. We performed the following experiments: (1) sequential assimilation of M3 and M5 with parts of well history divided according to the seismic acquisition dates; (2) assimilation of M3 with the entire well history and subsequent assimilation of M5; (3) assimilation of well and M3 data; and (4) assimilation of well and M5 data. For comparison purposes, we also assimilated only well data. From the results, we observed that well and 4DS data misfits were reduced when assimilating both monitors, compared to the cases where only a single monitor (any of them) was used with production data. This conclusion is also true in the comparison with results obtained when only assimilating well data. This indicates that both seismic monitors are important data to be quantitatively considered in DA. In this particular field, using a previous DA run to solely assimilate the newly available monitor (Case 2) delivered better models and long-term forecasts. Therefore, this would be our recommendation. This study highlights the importance of several 4DS acquisitions for reservoir monitoring and management and shows the challenges of their application in seismic DA for better life cycle field applications.
HeLa quality control sample preparation for MS-based proteomics
Authors: Webel Henry
Published: May 2023

Cell lysis and digestion protocol of HeLa cell line for quality control measurments of mass spectrometry-based proteomics measurments.
A technique for securing digital audio files based on rotation and XOR operations
Authors: Joshi Anand Ballabh , Gaffar Abdul
Published: May 2023

In this paper, we propose a WORD-oriented technique for encrypting (and decrypting) digital audio files based on rotation and XOR operations. The key-concepts of the designed encryption algorithm are the RX (Rotation-XOR) operations, i.e., the plain audio samples are first left-rotated by the sum-of-digits of the previous audio samples, and then XOR-ed with the previous audio samples. The designed encryption algorithm encodes a digital audio file into a random (noise) like audio file, from human visual as well as statistical points of view. Several encryption and decryption evaluation metrics, such as spectrogram, adjacent sample correlation coefficient, number of sample change rate, peak signal-to-noise ratio, etc., are applied on several digital audio files of varying sizes in order to empirically assess the performance and efficiency of the proposed technique. The results of these metrics validate the robustness of the designed technique.
Physiological and differential gene expression reveals a trade‐off between antioxidant capacity and salt tolerance in halophytes Urochondra setulosa and Dichanthium annulatum
Authors: MANN ANITA , Kumar Naresh , Lata Charu , Kumar Arvind , Meena B. L. , Kumar Ashwani
Published: May 2023

Background
Among abiotic stresses, soil salinity is one of the major global constraints to growth and productivity in most of the crop plants, limiting current and future agricultural sustainability. One of the strategies to dissect the salinity tolerance phenomenon can be the study of plants growing naturally in saline environments and halophytes can serve as another model plants for salt tolerance studies.
Methods and Results
Here, we studied two un-explored halophytes, moderately salt tolerant, Dichanthium annulatum and extremely salt tolerant, Urochondra setulosa for investigating the contributory role of antioxidative system, the first line of defence, in salinity tolerance mechanism at salinity levels of ECe ~ 30, 40 and 50 dSm− 1(~ 300,400,500 mM NaCl). H2O2 content, SOD and ascorbate peroxidase activities were higher in U. setulosa at all saline treatments whereas MDA content and catalase activity was high in D. annulatum although the specific enzyme activities of ROS system increased with increasing levels of salinity in both the halophytes. This differential physiological expression was in parallel with the transcriptomic data generated through High throughput sequencing on Illumina platform depicting 276 and 66 differentially expressed genes coding for various components of ROS system like antioxidant activity, cell redox and glutathione metabolism in response to salinity in U. setulosa and D. annulatum respectively. In D. annulatum, H2O2 is detoxified by increased activities of SOD, APX and catalase where as in halophyte U. setulosa, peroxidase takes over catalase to remove H2O2 along with DHAR and MDHAR which significantly correlates with the differentially expressed transcripts.
Conclusions
The salinity responsive gene expression for ROS enzymes and antioxidants clearly differentiate between these two halophytes supporting the detoxification of H2O2 and survival at different salinity levels. This study provides reference information on the key genes responsible for salt tolerance which can be used for related plant species for genetic improvement.
Advancing Understanding on Greenspace and Mental Health in Young People
Authors: Ryan Sophia C , Sugg Margaret M , Runkle Jennifer D , Thapa Bhuwan
Published: May 2023

Poor mental health outcomes among young people, including anxiety, depression, and suicide, have increased substantially in recent years. Given this concerning rise, more research into low-cost mental health interventions is needed. Research suggests that greenspace may be protective of mental health. This study aims to further understanding of the greenspace-mental health association among young people in five distinct urbanities (i.e., urban, suburban, micropolitan, small towns, rural/isolated). We apply publicly available greenspace datasets, which were used to derive greenspace quantity, quality, and accessibility metrics. Emergency department visits for young people (< 24 years) for the following mental health disorders were examined: anxiety, depression, mood disorders, mental and behavioral disorders, and substance use disorders. Generalized linear models investigated the association between greenspace and community-level drivers of mental health burden in North Carolina. Mood disorders saw the highest increase in prevalence in urban communities with low greenspace quantity (PRR: 1.19, CI: 1.16–1.21), anxiety disorders were associated with the greatest increase in rural/isolated communities with poor greenspace quality (PRR: 1.61, CI: 1.43–1.82), and both substance use disorders (PRR: 2.38, CI: 2.19–2.58) and depression (PRR: 2.09, CI: 1.72–2.53) were associated with the greatest increase in prevalence in rural/isolated communities with poor greenspace accessibility. Greenspace quantity interventions may be most effective in urban and suburban areas, greenspace quality interventions may be most beneficial in small towns and rural/isolated communities, and greenspace accessibility interventions may be most useful in urban, micropolitan and rural/isolated communities. Our analysis provides community-specific findings to guide targeted greenspace-mental health interventions.
Comparison of 0.01% and 0.05% Atropine Eye Drops in Childhood Myopia Progression
Authors: Akdemir Sibel Coşkun
Published: May 2023

Background: Comparison of 0.01% and 0.05% atropine eye drops with every other day treatment for slowing down myopia progression in children.
Methods: Children aged 5-16 years with myopia of -1.00-10.00 D and astigmatism less than -2.50 D and had myopic progression of at least 1.00D in the past year were included in the retrospective study. 0.01% and 0.05% atropine eye drops were applied every other day. At each visit, all patients underwent complete ophthalmic examination including cycloplegic refraction, axial length (AL), and photopic-mesopic pupil sizes. Spherical equivalent(SE), AL and photopic-mesopic pupil sizes were evaluated.
Results: Totally 92 eyes of 46 patients were included in the study ( 22 0.01% atropin, 24 0.05% atropin). At the end of the 12-months follow- up period, tbe mean SE change was -0.41±0.28 D and -0,19±0.22 D in the 0.01% and %0.05 atropine groups, respectively (p<0.001). AL change was 0.19±0.16mm and 0.16±0.24mm in the 0.01% and 0.05% atropine groups, respectively (p=0.52). Side effects such as photophobia and blurred near vision were not observed in the 0.01% atropine group, but in the 0.05% atropine group, photophobia ( 3 patients) and blurred near vision ( 8 patients) were observed.
Conclusions: 0.01% and 0.05% atropine eye drops significantly slow down the progression of myopia with every other day use. 0.01% atropine is better tolerated than 0.05% atropine.
Recurring EPHB1 mutations in human cancers alter receptor signalling and compartmentalisation of colorectal cancer cells
Authors: Kundu Snehangshu , Nunes Luis , Adler Jeremy , Mathot Lucy , Stoimenov Ivaylo , Sjöblom Tobias
Published: May 2023

Background: Ephrin (EPH) receptors have been implicated in tumorigenesis and metastasis, but the functional understanding of mutations observed in human cancers is limited. We previously demonstrated reduced cell compartmentalisation for somatic EPHB1 mutations found in metastatic colorectal cancer cases. We therefore integrated pan-cancer and pan-EPH mutational data to prioritise recurrent EPHB1mutations for functional studies to understand their contribution to cancer development and metastasis.
Methods: Here, 79,151 somatic mutations in 9,898 samples of 33 different tumour types were analysed with a bioinformatic pipeline to find 3D-mutated cluster pairs and recurring hotspot mutations in EPHreceptors. From these, 15 recurring EPHB1mutations were stably expressed in colorectal cancer followed by 3D confocal microscopy based in vitro compartmentalisation assays and phospho-proteome analysis using the Sciomics platform.
Results: Whereas the ligand-binding domain mutations C61Y, R90C, and R170W, the fibronectin domain mutation R351L, and the kinase domain mutation D762N displayed reduced to strongly compromised cell compartmentalisation, the kinase domain mutations R743W and G821R enhanced this phenotype. While mutants with reduced compartmentalisation also had reduced ligand induced receptor phosphorylation, the enhanced compartmentalisation was not linked to receptor phosphorylation level. Phosphoproteome mapping pinpointed the PI3K pathway and PIK3C2B phosphorylation in cells harbouring mutants with reduced compartmentalisation. Furthermore, the 3D-protein structure-based bioinformatics analysis showed comparatively more robustness by identifying 63% (5 out of 8 selected 3D-anlysed mutants) vs the 43% (3 out of 7 selected 2D-analysed mutants) EPHB1mutants with compartmentalisation phenotypes.
Conclusions: This is the first integrative study of pan-cancer EPH receptor mutations followed by in vitro validation, a robust way to identify cancer-causing mutations, and demonstrated the utility for 3D-protein structure-based mutation analysis in characterization of putative cancer genes.
Exploring the relationship between education, living environment, and anxiety/depression among stable patients: insights from the COPD-AD China Registry study
Authors: Zhao Xuanna , Long Bingyu , Huang Bangxiao , Zhong Xiaomei , Chen Xiaoer , Shen Xiaoping , Huang Qiu , Zhang Wenchao , Wu Jiayuan , Chen Min , Li Dongming , Wu Bin , Lv Dong , Huang Dan , Wu Dong
Published: May 2023

Background
The relationship between education and living environment is complex, and both factors are related to mental health. However, few studies have examined the independent and combined effects of education and living environment on mental health among patients with chronic obstructive pulmonary disease (COPD).
Methods
We analyzed data for 1064 patients with COPD enrolled in the COPD-AD China Registry study from 2017 to 2021. Binary logistic regression was used to analyze the independent and combined effects of education and living environment on patients’ mental health. We also evaluated additive interactions with the relative excess risk ratio (RERI), attribution percentage (AP), and synergy index (SI).
Results
Our primary analysis revealed the incidence of anxiety and depression was associated with educational level and living environment. After adjustment for potential confounding factors, binary logistic regression analyses showed that low education level and urban living environment were independently associated with higher risks for anxiety (odds ratio [OR]: 1.56, 95% confidence interval [CI] 1.06–2.29 and OR:2.15, 95% CI 1.51–2.05) and depression (OR:1.62, 95% CI 1.17–2.27 and OR: 2.01, 95% CI 1.46–2.75) among patients with COPD. Our secondary analysis showed that the combination of low education level and living in urban areas was associated with the highest and significantly increased risk for anxiety (OR: 7.90, 95% CI 3.83–16.29, P < .001) or depression (OR: 11.79, 95% CI 5.77–24.10, P < .001) among these patients. Furthermore, we observed strong synergistic additive interactions between low education level and living in urban areas for anxiety (SI: 11.57, 95% CI 1.41–95.27; RERI: 6.31, 95% CI 1.60–11.01; AP: 0.8, 95% CI 0.66–0.94) and depression (SI: 31.31, 95% CI 1.59–617.04; RERI: 10.44, 95% CI 2.66–18.23; AP: 0.89, 95% CI 0.8–0.97).
Conclusions
Our study suggests that mental health among patients with COPD is influenced by their education and living environment. Patients who have low education levels and live in urban areas may experience more anxiety and depression.
Trial registration
The clinical trial registration code was obtained from the ClinicalTrials.gov (date of registration: 14/06/2017, registration number: NCT03187236).
Osteoporosis and associated risk factors in patients with severe hemophilia A: a case- control study from China
Authors: Wu Dongxiao , Shen ShaoNing
Published: May 2023

IntroductionPeople with hemophilia risk osteoporosis more than healthy people, which may be related to specific factors.MethodsThis case-control study included 53 patients with severe hemophilia type A and 49 healthy participants. Dual-energy X-ray absorptiometry was used to determine bone mineral density (BMD). Collected Information on age, body mass index (BMI), number of deformed joints, Functional Independence Score in Hemophilia (FISH), bone turnover markers, antibodies, treatment modalities. To identify independent risk factors for osteoporosis.ResultsThe BMD of the femoral neck (0.80g/cm2vs.0.97 g/cm2), Ward’s triangle (0.62 g/cm2vs.0.83 g/cm2), tuberosity (0.63 g/cm2vs.0.80 g/cm2) and hip (0.80g/cm2vs.0.98 g/ cm2) in the case group were significantly lower than those in the control group, all of which were P < 0.001. However, there was no significant difference in the overall BMD of lumbar spine(L1-L4) (1.07g / cm2vs. 1.11g / cm2). The incidence of osteoporosis in the case group was 41.51%. BMI and FISH score were considered as independent risk factors for BMD decrease.ConclusionThe BMD of patients with severe hemophilia A is much lower than that of healthy population, and this difference is mainly reflected in the hip. The clear influencing factors were low BMI and functional independence decrease. Osteoclast was active while osteoblast activity was not enhanced synchronously, which may be the pathological mechanism of BMD decrease.
Dietary intakes of women with gestational diabetes mellitus and pregnancy outcomes: a prospective observational study
Authors: Zheng Xin , Zhang Qiaoqing , Su Weijuan , Liu Wei , Huang Caoxin , Shi Xiulin , Li Xuejun
Published: May 2023

Background
Nutritient intake of pregnant women with gestational diabetes mellitus (GDM) is important to ensure satisfactory birth outcomes. The aim of this study is to explore the dietary profiles of patients with GDM, compare the results with the Chinese dietary guidelines or Dietary Reference Intakes (DRIs) from China and investigate the relationship between maternal dietary intake and pregnancy outcomes.
Methods
A total of 221 patients with GDM in the second trimester were included in the cohort. Dietary intake data were collected using a 24-hour recall method for 3 consecutive days. Pregnancy outcomes of these participants were followed up. Univariate logistic regression and multivariate logistic regression analyses were conducted to explore the associations between dietary intake variables or general characteristics variables and adverse pregnancy outcomes.
Results
Participants with adverse pregnancy outcomes showed lower percentage of dietary energy intake from carbohydrate, iodine and Vitamin D and higher percentage of dietary energy intake from fat compared to participants without adverse pregnancy outcomes. The gestational weight gain and family history of diabetes were associated with increased risk of adverse pregnancy outcomes. Exercise, the percentage of dietary energy intake from carbohydrate, the intake of iodine and Vitamin D were associated with a decreased risk of adverse pregnancy outcomes.
Conclusion
The daily diet of pregnant women with GDM in China did not meet the dietary guidelines or DRIs. The low dietary carbohydrate ratio and intake of Vitamin D and iodine, family history of diabetes, lack of exercise, and high gestational weight gain were associated with increased risk of adverse pregnancy outcomes in pregnant women with GDM.
The effect of birth ball exercise on labor pain, delivery duration, birth comfort, and birth satisfaction: A randomized controlled study
Authors: Aslantaş Beyza Nur , Çankaya Seyhan
Published: May 2023

Purpose
This study aimed to examine the effect of birth ball exercise on labor pain, delivery duration, birth comfort, and birth satisfaction.
Methods
The study used a randomized controlled trial design. All 120 primipara pregnant women were randomly assigned to the intervention (IG) and control groups (CG). After the cervical dilatation reached at 4 cm, the pregnant women in the IG performed birth ball exercises, adhering to the birth ball guide created by the researcher. No intervention was made in the control group other than standard midwifery care practices.
Results
The labor pain level between the groups (VAS 1-when cervical dilatation was 4 cm) was similar to each other. The labor pain level (VAS 2- when cervical dilatation was 9 cm) scores of the women in the IG were significantly lower than those in the CG (p < 0.05). The time between the active phase of labor until dilatation is complete and the time until the baby's head comes out after full dilatation was found to be statistically significantly shorter in the IG compared to the CG (p < 0.05). Childbirth comfort and satisfaction mean scores between the groups were found to be statistically insignificant (p > 0.05).
Conclusion
As a result of the study, it was determined that the birth ball exercise significantly reduced labor pain and labor time. We recommend that the birth ball exercise be applied to all low-risk pregnant women because it helps fetal descent and cervical dilatation, and shortens labor pain and delivery time.
Appropriateness of Prostate Cancer Prevention and Screening Recommendations Obtained From ChatGPT-4
Authors: Zheng Yuxiao , Xu Zicheng , Yu Bin , Xu Ting , Huang Xiaochen , Zou Qing , Peng Bo
Published: May 2023

Purpose: To evaluate the appropriateness of prostate cancer (PCa) prevention and screening recommendations provided by ChatGPT-4
Materials and Methods: We presented 32 questions regarding PCa prevention and screening using ChatGPT-4 based on National Comprehensive Cancer Network (NCCN) guidelines and clinical experience. Each question was asked to ChatGPT-4 three times, and the responses were graded by three expert urologists as reviewers. We evaluated sets of three responses generated by ChatGPT-4 in two distinct contexts: a general patient-facing platform and direct patient-to-clinician messages. A board certified/eligible urologist assessed each response set as appropriate, inappropriate, or unreliable.
Results: In both contexts, ChatGPT-4 generated appropriate responses for 30 out of 32 (94%) questions, while one (3%) was considered inappropriate and one (3%) was deemed unreliable. Inappropriate response was related to a Gleason score of 5+3, while unreliable answer was linked to a Gleason score of 3+5 on prostate biopsy results.
Conclusions: ChatGPT-4 is capable of providing appropriate counseling regarding disease prevention and screening for patients with PCa.