bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of a SYBR Green qPCR Intralaboratory Validation for the Quantification of Escherichia coli O157:H7
Authors: Yepes-Pérez María , Carrero-Contreras Karent , Vásquez-Araque Neil A. , Mora Martínez Amanda Lucía , Correa-Londoño Guillermo A. , Leotta Gerardo
Published: August 2024

Escherichia coli serotype O157:H7 is a diarrheal agent and a significant cause of hemorrhagic colitis and the development of hemolytic uremic syndrome (HUS), mainly in infants. Early detection of contaminated food and water using reliable and fast tests is one of the strategies to prevent infections from E. coli O157:H7. Methods: Four quantitative polymerase chain reaction protocols (SYBR Green qPCR) were developed and validated to determine the presence of the bacteria according to its rfbE, stx1, and stx2 genes. Results: The results of the efficiencies were between 80% and 97% with a high linearity (R2 0.99). The cut-off limits for each primer sequence were 3.1667 × 10−2 ng µL−1 for two sequences of the serogroup O157 (primers rfbE and O157), 1.7228 × 10−3 ng µL−1 for stx1, and 3.5185 × 10−3 ng µL−1 for stx2. The inclusivity and the exclusivity of each gene, as well as the analytical precision and the positive and negative predictive value, were 100%. A contaminated meat matrix was evaluated, detecting up to 4 CFU g−1. Conclusions: SYBR Green qPCR protocols could be implemented to trace the presence of E. coli O157 in a routine analysis of ground beef or as an easy, rapid, sensitive, and specific diagnostic test while still considering microbiological tests to validate any inconclusive results.
Solving Complex Optimisation Problems by Machine Learning
Authors: Prestwich Steven
Published: August 2024

Most optimisation research focuses on relatively simple cases: one decision maker, one objective, and possibly a set of constraints. However, real-world optimisation problems often come with complications: they might be multi-objective, multi-agent, multi-stage or multi-level, and they might have uncertainty, partial knowledge or nonlinear objectives. Each has led to research areas with dedicated solution methods. However, when new hybrid problems are encountered, there is typically no solver available. We define a broad class of discrete optimisation problem called an influence program, and describe a lightweight algorithm based on multi-agent multi-objective reinforcement learning with sampling. We show that it can be used to solve problems from a wide range of literatures: constraint programming, Bayesian networks, stochastic programming, influence diagrams (standard, limited memory and multi-objective), and game theory (multi-level programming, Bayesian games and level-k reasoning). We expect it to be useful for the rapid prototyping of solution methods for new hybrid problems.
“Modern and Contemporary Art: Topical Abstraction in Contemporary Sculpture” Special Issue Introduction
Authors: Speaks Elyse , Richmond Susan
Published: August 2024

The essays gathered in this Special Issue of Arts concern artists working in the United States and Europe since the 1960s who have leveraged sculptural abstraction to address topical issues without ceding to the classical framework of figuration [...]
Research of Air Purification Using Ion Energy Effect on Particulate Matter Agglomeration
Authors: Čereška Audrius , Tetsmann Ina , Bareikis Regimantas , Jasevičius Raimondas
Published: August 2024

The article presents a special stand featuring ion generators designed and manufactured for experimental research. This study investigates the use of ion energy for air purification, specifically focusing on its effect on PM (0.3–10 μm) agglomeration. By introducing charged ions into the air, particulate matter can be charged, resulting in increased particle sizes through agglomeration, and subsequently improving the filtration efficiency. Experiments were conducted in a controlled chamber to evaluate the performance of an ion-based air purification system. The effects of various parameters, such as ion concentration, airflow rate, and particle size distribution, on PM agglomeration were studied. The results demonstrated that the ion-based air purification system could effectively enhance PM agglomeration, particularly for ultrafine particles. The study also revealed that the efficiency of PM agglomeration was influenced by the ion concentration and airflow rate, with an optimal range identified to maximize the system’s performance. Furthermore, the potential real-world application of this technology was discussed, considering factors such as energy consumption, maintenance requirements, and compatibility with existing air purification systems. This study contributes to the development of innovative air purification solutions and offers valuable insights into the potential of ion energy for improving indoor air quality and safeguarding human health.
Molecular Pathways and Potential Therapeutic Targets of Refractory Asthma
Authors: Ishmael Leah , Casale Thomas , Cardet Juan Carlos
Published: August 2024

Asthma is a chronic inflammatory lung disease. Refractory asthma poses a significant challenge in management due to its resistance to standard therapies. Key molecular pathways of refractory asthma include T2 inflammation mediated by Th2 and ILC2 cells, eosinophils, and cytokines including IL-4, IL-5, and IL-13. Additionally, non-T2 mechanisms involving neutrophils, macrophages, IL-1, IL-6, and IL-17 mediate a corticosteroid resistant phenotype. Mediators including alarmins (IL-25, IL-33, TSLP) and OX40L have overlap between T2 and non-T2 inflammation and may signify unique pathways of asthma inflammation. Therapies that target these pathways and mediators have proven to be effective in reducing exacerbations and improving lung function in subsets of severe asthma patients. However, there are patients with severe asthma who do not respond to approved therapies. Small molecule inhibitors, such as JAK-inhibitors, and monoclonal antibodies targeting mast cells, IL-1, IL-6, IL-33, TNFα, and OX40L are under investigation for their potential to modulate inflammation involved in refractory asthma. Understanding refractory asthma heterogeneity and identifying mediators involved are essential in developing therapeutic interventions for patients unresponsive to currently approved biologics. Further investigation is needed to develop personalized treatments based on these molecular insights to potentially offer more effective treatments for this complex disease.
Using Citizen Science and Field Surveys to Document the Introduction, Establishment, and Rapid Spread of the Bare-Eyed Pigeon, Patagioenas corensis, on the Island of Saint-Martin, West Indies
Authors: Cambrone Christopher , Levesque Anthony , Cézilly Frank
Published: August 2024

Early reporting of the introduction and establishment of exotic species is of paramount importance for successful management. Here, we report the introduction and rapid spread of the Bare-eyed Pigeon, Patagioenas corensis, on the binational island of Saint-Martin, the West Indies. This range-restricted species naturally occurs in arid coastal areas of Columbia and Venezuela and nearby islands. Its introduction on Saint-Martin represents an expansion of about 1000 km beyond its established native range. Using observations recorded in eBird and results from a recent field survey, we show that since its introduction, most probably between late 2012 and early 2013, the species has expanded fast in Saint-Martin and has recently broadened its habitat to include anthropized, built areas. The expansion of Bare-eyed Pigeon on Saint-Martin and the neighboring Leeward Islands, possibly facilitated by climate change in the future, could be a threat to both native columbid species and other bird species through competition for resources. We therefore recommend that local authorities and stakeholders rapidly eradicate the species or at least prevent its further spread on Saint-Martin, possibly though listing it as a game species, while it is still possible to do so.
CPT2 Deficiency Modeled in Zebrafish: Abnormal Neural Development, Electrical Activity, Behavior, and Schizophrenia-Related Gene Expression
Authors: Baker Carly E. , Marta Aaron G. , Zimmerman Nathan D. , Korade Zeljka , Mathy Nicholas W. , Wilton Delaney , Simeone Timothy , Kochvar Andrew , Kramer Kenneth L. , Stessman Holly A. F. , Shibata Annemarie
Published: August 2024

Carnitine palmitoyltransferase 2 (CPT2) is an inner mitochondrial membrane protein of the carnitine shuttle and is involved in the beta-oxidation of long chain fatty acids. Beta-oxidation provides an alternative pathway of energy production during early development and starvation. CPT2 deficiency is a genetic disorder that we recently showed can be associated with schizophrenia. We hypothesize that CPT2 deficiency during early brain development causes transcriptional, structural, and functional abnormalities that may contribute to a CNS environment that is susceptible to the emergence of schizophrenia. To investigate the effect of CPT2 deficiency on early vertebrate development and brain function, CPT2 was knocked down in a zebrafish model system. CPT2 knockdown resulted in abnormal lipid utilization and deposition, reduction in body size, and abnormal brain development. Axonal projections, neurotransmitter synthesis, electrical hyperactivity, and swimming behavior were disrupted in CPT2 knockdown zebrafish. RT-qPCR analyses showed significant increases in the expression of schizophrenia-associated genes in CPT2 knockdown compared to control zebrafish. Taken together, these data demonstrate that zebrafish are a useful model for studying the importance of beta-oxidation for early vertebrate development and brain function. This study also presents novel findings linking CPT2 deficiency to the regulation of schizophrenia and neurodegenerative disease-associated genes.
Endoplasmic Reticulum Stress-Mediated Cell Death in Renal Fibrosis
Authors: Guo Shangze , Tong Yinghao , Li Ting , Yang Kexin , Gao Wei , Peng Fujun , Zou Xiangyu
Published: August 2024

The endoplasmic reticulum (ER) is indispensable for maintaining normal life activities. Dysregulation of the ER function results in the accumulation of harmful proteins and lipids and the disruption of intracellular signaling pathways, leading to cellular dysfunction and eventual death. Protein misfolding within the ER disrupts its delicate balance, resulting in the accumulation of misfolded or unfolded proteins, a condition known as endoplasmic reticulum stress (ERS). Renal fibrosis, characterized by the aberrant proliferation of fibrotic tissue in the renal interstitium, stands as a grave consequence of numerous kidney disorders, precipitating a gradual decline in renal function. Renal fibrosis is a serious complication of many kidney conditions and is characterized by the overgrowth of fibrotic tissue in the glomerular and tubular interstitium, leading to the progressive failure of renal function. Studies have shown that, during the onset and progression of kidney disease, ERS causes various problems in the kidneys, a process that can lead to kidney fibrosis. This article elucidates the underlying intracellular signaling pathways modulated by ERS, delineating its role in triggering diverse forms of cell death. Additionally, it comprehensively explores a spectrum of potential pharmacological agents and molecular interventions aimed at mitigating ERS, thereby charting novel research avenues and therapeutic advancements in the management of renal fibrosis.
Comprehensive Analysis of Kisspeptin Signaling: Effects on Cellular Dynamics in Cervical Cancer
Authors: Rodríguez-Sarmiento Deisy Yurley , Rondón-Villarreal Paola , Scarpelli-Pereira Pedro Henrique , Bouvier Michel
Published: August 2024

Kisspeptin, a key neuropeptide derived from the KISS1R gene, is renowned for its critical role in regulating the hypothalamic–pituitary–gonadal axis and reproductive hormone secretion. Beyond its primary function in reproductive biology, emerging research has illuminated its influence in various cancers, mediating significant effects through its interaction with the G protein-coupled receptor, kisspeptin receptor. This interaction has been implicated in modulating cellular processes such as proliferation and metastasis, making it a potential target for therapeutic intervention. Our study initially screened ten kisspeptin-10 analogs through cytotoxic effects of kisspeptin-10 (KP10) and its analogs in several cancer types, including cervical, prostate, breast, and gastric cancers, with a particular focus on cervical cancer, where the most profound effects were observed. Further exploration using kinase array assays revealed that these analogs specifically alter key kinases involved in cancer progression. Migration assays demonstrated a substantial decrease in cell motility, and Bioluminescence Resonance Energy Transfer assays confirmed these analogs’ strong interactions with the kisspeptin receptor. Overall, our results indicate that these KP10 analogs not only hinder cervical cancer cell proliferation but also curtail migration through targeted modulation of kinase signaling, suggesting their potential as therapeutic agents in managing cervical cancer progression. This comprehensive approach underscores the therapeutic promise of exploiting kisspeptin signaling in cancer treatment strategies.
Plasma microRNA Environment Linked to Tissue Factor Pathway and Cancer-Associated Thrombosis: Prognostic Significance in Ovarian Cancer
Authors: Tavares Valéria , Savva-Bordalo Joana , Rei Mariana , Liz-Pimenta Joana , Assis Joana , Pereira Deolinda , Medeiros Rui
Published: August 2024

Ovarian cancer (OC) is a leading cause of death among gynaecological malignancies. The haemostatic system, which controls blood flow and prevents clotting disorders, paradoxically drives OC progression while increasing the risk of venous thromboembolism (VTE). MicroRNAs (miRNAs) have emerged as crucial in understanding VTE pathogenesis. Exploring the connection between cancer and thrombosis through these RNAs could lead to novel biomarkers of cancer-associated thrombosis (CAT) and OC, as well as potential therapeutic targets for tumour management. Thus, this study examined the impact of eight plasma miRNAs targeting the tissue factor (TF) coagulation pathway—miR-18a-5p, -19a-3p, -20a-5p, -23a-3p, -27a-3p, -103a-3p, -126-5p and -616-3p—in 55 OC patients. Briefly, VTE occurrence post-OC diagnosis was linked to shorter disease progression time (log-rank test, p = 0.024) and poorer overall survival (OS) (log-rank test, p < 0.001). High pre-chemotherapy levels of miR-20a-5p (targeting coagulation factor 3 (F3) and tissue factor pathway inhibitor 2 (TFPI2)) and miR-616-3p (targeting TFPI2) predicted VTE after OC diagnosis (χ2, p < 0.05). Regarding patients’ prognosis regardless of VTE, miR-20a-5p independently predicted OC progression (adjusted hazard ratio (aHR) = 6.13, p = 0.005), while miR-616-3p significantly impacted patients’ survival (aHR = 3.72, p = 0.020). Further investigation is warranted for their translation into clinical practice.
The Physiological Impact of Melatonin, Its Effect on the Course of Diseases and Their Therapy and the Effect of Magnetic Fields on Melatonin Secretion—Potential Common Pathways of Influence
Authors: Woldańska-Okońska Marta , Koszela Kamil
Published: August 2024

Melatonin is a relic, due to its millions-of-years-old presence in chemical reactions, found in evolutionarily diverse organisms. It has a multidirectional biological function. It controls diurnal rhythms, redox homeostasis, intestinal motor functions, mitochondrial biogenesis and fetal development and has antioxidant effects. It also has analgesic and therapeutic effects. The purpose of this paper is to describe the role of melatonin in vital processes occurring in interaction with the environment, with particular reference to various magnetic fields ubiquitous in the life of animate matter, especially radio frequency/extra low frequency (RF/ELF EMF) and static magnetic fields. The most important part of this article is to describe the potential effects of magnetic fields on melatonin secretion and the resulting possible health effects. Melatonin in some cases positively amplifies the electromagnetic signal, intensifying health effects, such as neurogenesis, analgesic effects or lowering blood pressure. In other cases, it is a stimulus that inhibits the processes of destruction and aggravation of lesions. Sometimes, however, in contrast to the beneficial effects of electromagnetic fields in therapy, they intensify pathogenic effects, as in multiple sclerosis by intensifying the inflammatory process.
Effects of Synthetic Toll-Like Receptor 9 Ligand Molecules on Pulpal Immunomodulatory Response and Repair after Injuries
Authors: Quispe-Salcedo Angela , Yamazaki Tomohiko , Ohshima Hayato
Published: August 2024

Synthetic oligodeoxynucleotides (ODNs) containing unmethylated cytosine–phosphate–guanine (CpG) motifs (CpG-ODNs) are ligand molecules for Toll-like receptor 9 (TLR9), which is expressed by odontoblasts in vitro and dental pulp cells. This study determined the effects of CpG-ODNs on pulpal immunomodulatory response and repair following injury. Briefly, the upper right first molars of three-week-old mice were extracted, immersed in Type A (D35) or B (K3) CpG-ODN solutions (0.1 or 0.8 mM) for 30 min, and then replanted. Pulpal healing and immunomodulatory activity were assessed by hematoxylin–eosin and AZAN staining, as well as immunohistochemistry. One week following the operation, inflammatory reactions occurred in all of the experimental groups; however, re-revascularization and newly formed hard tissue deposition were observed in the pulp chamber of all groups at week 2. A positive trend in the expression of immune cell markers was observed toward the CpG-ODN groups at 0.1 mM. Our data suggest that synthetic CpG-ODN solutions at low concentrations may evoke a long-lasting macrophage–TLR9-mediated pro-inflammatory, rather than anti-inflammatory, response in the dental pulp to modulate the repair process and hard tissue formation. Further studies are needed to determine the effects of current immunomodulatory agents in vitro and in vivo and develop treatment strategies for dental tissue regeneration.
Molecular Mimicry between Toxoplasma gondii B-Cell Epitopes and Neurodevelopmental Proteins: An Immunoinformatic Approach
Authors: Meza-Sosa Karla F. , Valle-Garcia David , González-Conchillos Hugo , Blanco-Ayala Tonali , Salazar Alelí , Flores Itamar , Gómez-Manzo Saúl , González Esquivel Dinora Fabiola , Pérez de la Cruz Gonzalo , Pineda Benjamín , Pérez de la Cruz Verónica
Published: August 2024

Epidemiological studies and meta-analyses have shown a strong association between high seroprevalence of Toxoplasma gondii (T. gondii) and schizophrenia. Schizophrenic patients showed higher levels of anti-Toxoplasma immunoglobulins M and G (IgM and IgG) when compared to healthy controls. Previously, in a rat model, we demonstrated that the progeny of mothers immunized with T. gondii lysates before gestation had behavioral and social impairments during adulthood. Therefore, we suggested that T. gondii infection can trigger autoreactivity by molecularly mimicking host brain proteins. Here, we aimed to identify the occurrence of antigenic mimicry between T. gondii epitopes and host brain proteins. Using a bioinformatic approach, we predicted T. gondii RH-88 B cell epitopes and compared them to human cell-surface proteins involved in brain development and differentiation (BrainS). Five different algorithms for B-cell-epitope prediction were used and compared, resulting in 8584 T. gondii epitopes. We then compared T. gondii predicted epitopes to BrainS proteins by local sequence alignments using BLASTP. T. gondii immunogenic epitopes significantly overlapped with 42 BrainS proteins. Among these overlapping proteins essential for brain development and differentiation, we identified HSP90 and NOTCH receptors as the proteins most likely to be targeted by the maternally generated pathogenic antibodies due to their topological overlap at the extracellular region of their sequence. This analysis highlights the relevance of pregestational clinical surveillance and screening for potential pathogenic anti-T. gondii antibodies. It also identifies potential targets for the design of vaccines that could prevent behavioral and cognitive impairments associated with pre-gestational T. gondii exposure.
Bioactive Peptides in Dairy Milk: Highlighting the Role of Melatonin
Authors: Andrani Melania , Dall’Olio Eleonora , De Rensis Fabio , Tummaruk Padet , Saleri Roberta
Published: August 2024

Melatonin, an endogenous indolamine derived from tryptophan, is primarily synthesized by the pineal gland in mammals and regulated by a complex neural system. Its release follows a circadian rhythm, which is crucial for regulating physiological processes in response to light–dark cycles in both humans and animals. In this review, we report that the presence of this hormone in bovine milk, with significant differences in concentration between daytime and nighttime milking, has increased interest in milk as a natural source of bioactive molecules. Melatonin lowers cortisol levels at night, reduces body temperature and blood pressure, coinciding with decreased alertness and performance, acts as an antioxidant and anti-inflammatory agent, modulates the immune system, offers neuroprotective benefits, and supports gastrointestinal health by scavenging free radicals and reducing oxidative stress in dairy cows. Many factors influence the release of melatonin, such as the intensity of artificial lighting during nighttime milking, the frequency of milkings, milk yield, and genetic differences between animals. Nocturnal milking under low-intensity light boosts melatonin, potentially reducing oxidative damage and mastitis risk. Additionally, ultra-high temperature (UHT) treatment does not significantly affect the melatonin content in milk. However, further research on its stability during milk processing and storage is crucial for ensuring product efficacy. In some countries, nighttime milk with naturally elevated melatonin content is already commercialized as a natural aid for sleep. Thus, naturally melatonin-rich milk may be a promising alternative to synthetic supplements for promoting better sleep and overall well-being.
Proteomic Characterisation of Heart Failure Reveals a Unique Molecular Phenotype for Hypertrophic Cardiomyopathy
Authors: Tonry Claire , Linden Katie , Collier Patrick , Ledwidge Mark , McDonald Ken , Collins Ben C. , Watson Chris J.
Published: August 2024

Hypertrophic cardiomyopathy (HCM) is a disease, which is difficult to diagnose at an early stage and for which there is a pressing need for more effective treatment options. The purpose of this study was to compare the molecular profile of HCM to that of ischaemic cardiomyopathy (ISCM) and dilated cardiomyopathy (DCM) for identification of protein and pathway targets that could support the development of better diagnostic and treatment options for HCM. A high-throughput mass spectrometry workflow was applied to achieve deep quantitative coverage of left ventricular tissue from HCM, DCM, ISCM and non-heart-failure control patients. HCM had a diverse proteomic profile compared to that of DCM and ISCM. Differentially expressed proteins unique to HCM were identified based on an observed fold change of ≥1.5 or ≤0.67 and q-value ≤ 0.05. Candidate proteins of interest were found to be significantly associated with clinical features of HCM. The significant association between these proteins and HCM was validated in an independent dataset. This represents one of the largest and deepest proteomic datasets for myocardial tissue reported to date. The dataset highlights the diverse proteomic profile of HCM, relative to other cardiomyopathies, and reveals disease-relevant pathways and promising biomarker candidates that are uniquely associated with HCM.
Using microRNAs Networks to Understand Pancreatic Cancer—A Literature Review
Authors: Przybyszewski Oskar , Mik Michał , Nowicki Michał , Kusiński Michał , Mikołajczyk-Solińska Melania , Śliwińska Agnieszka
Published: August 2024

Pancreatic cancer is a severe disease, challenging to diagnose and treat, and thereby characterized by a poor prognosis and a high mortality rate. Pancreatic ductal adenocarcinoma (PDAC) represents approximately 90% of pancreatic cancer cases, while other cases include neuroendocrine carcinoma. Despite the growing knowledge of the pathophysiology of this cancer, the mortality rate caused by it has not been effectively reduced. Recently, microRNAs have aroused great interest among scientists and clinicians, as they are negative regulators of gene expression, which participate in many processes, including those related to the development of pancreatic cancer. The aim of this review is to show how microRNAs (miRNAs) affect key signaling pathways and related cellular processes in pancreatic cancer development, progression, diagnosis and treatment. We included the results of in vitro studies, animal model of pancreatic cancer and those performed on blood, saliva and tumor tissue isolated from patients suffering from PDAC. Our investigation identified numerous dysregulated miRNAs involved in KRAS, JAK/STAT, PI3/AKT, Wnt/β-catenin and TGF-β signaling pathways participating in cell cycle control, proliferation, differentiation, apoptosis and metastasis. Moreover, some miRNAs (miRNA-23a, miRNA-24, miRNA-29c, miRNA-216a) seem to be engaged in a crosstalk between signaling pathways. Evidence concerning the utility of microRNAs in the diagnosis and therapy of this cancer is poor. Therefore, despite growing knowledge of the involvement of miRNAs in several processes associated with pancreatic cancer, we are beginning to recognize and understand their role and usefulness in clinical practice.
Renalase Potential as a Marker and Therapeutic Target in Chronic Kidney Disease
Authors: Serban-Feier Larisa Florina , Cuiban Elena , Gogosoiu Elena Bianca , Stepan Elena , Radulescu Daniela
Published: August 2024

Hypertension and cardiovascular disease are prominent features of chronic kidney disease, and they are associated with premature mortality and progression toward end-stage kidney disease. Renalase, an enzyme secreted predominantly by the kidney and identified in 2005, seems to be one of the missing pieces in the puzzle of heart and kidney interaction in chronic kidney disease by lowering blood pressure and reducing the overactivity of sympathetic tone. This review aims to summarize evidence from clinical studies performed on subjects with CKD in order to explore the value of renalase as a marker and/or a therapeutic target in this disease.
Inflammaging: The Next Challenge—Exploring the Role of Gut Microbiota, Environmental Factors, and Sex Differences
Authors: Caldarelli Mario , Rio Pierluigi , Marrone Andrea , Giambra Vincenzo , Gasbarrini Antonio , Gambassi Giovanni , Cianci Rossella
Published: August 2024

The term ‘inflammaging’ has been coined to describe the chronic state of inflammation derived from ongoing cycles of tissue damage and the subsequent immune responses. This inflammatory status contributes to the decline of organs and physiological functions, accelerates the aging process, and increases the risk of age-related illnesses and death. During aging, the gut microbiota (GM) undergoes significant changes, including a decreased diversity of species, a decline in beneficial bacteria, and a rise in proinflammatory ones, resulting in persistent low-grade inflammation. Moreover, environmental factors, such as diet and medications, contribute to age-related changes in GM and immune function, preventing or promoting inflammaging. This narrative review aims to clarify the underlying mechanisms of inflammaging and to specifically investigate the influence of GM and several environmental factors on these mechanisms, while also exploring potential differences related to sex. Moreover, lifestyle and pharmacological interventions will be suggested to promote healthy aging.
Cellular and Molecular Mechanisms Underlying Altered Excitability of Cardiac Efferent Neurons in Cirrhotic Rats
Authors: Lee Choong-Ku , Nguyen Huu Son , Kang Seong Jun , Jeong Seong-Woo
Published: August 2024

Patients with cirrhosis often exhibit cardiac autonomic dysfunction (CAD), characterized by enhanced cardiac sympathetic activity and diminished cardiac vagal tone, leading to increased morbidity and mortality. This study delineates the cellular and molecular mechanisms associated with altered neuronal activities causing cirrhosis-induced CAD. Biliary and nonbiliary cirrhotic rats were produced by common bile duct ligation (CBDL) and intraperitoneal injections of thioacetamide (TAA), respectively. Three weeks after CBDL or TAA injection, the assessment of heart rate variability revealed autonomic imbalance in cirrhotic rats. We observed increased excitability in stellate ganglion (SG) neurons and decreased excitability in intracardiac ganglion (ICG) neurons in cirrhotic rats compared to sham-operated controls. Additionally, threshold, rheobase, and action potential duration exhibited opposite alterations in SG and ICG neurons, along with changes in afterhyperpolarization duration. A- and M-type K⁺ channels were significantly downregulated in SG neurons, while M-type K⁺ channels were upregulated, with downregulation of the N- and L-type Ca2⁺ channels in the ICG neurons of cirrhotic rats, both in transcript expression and functional activity. Collectively, these findings suggest that cirrhosis induces an imbalance between cardiac sympathetic and parasympathetic neuronal activities via the differential regulation of K+ and Ca2+ channels. Thus, cirrhosis-induced CAD may be associated with impaired autonomic efferent functions within the homeostatic reflex arc that regulates cardiac functions.
Unraveling the Complex Molecular Interplay and Vascular Adaptive Changes in Hypertension-Induced Kidney Disease
Authors: Gaydarski Lyubomir , Dimitrova Iva N. , Stanchev Stancho , Iliev Alexandar , Kotov Georgi , Kirkov Vidin , Stamenov Nikola , Dikov Tihomir , Georgiev Georgi P. , Landzhov Boycho
Published: August 2024

Angiogenesis, the natural mechanism by which fresh blood vessels develop from preexisting ones, is altered in arterial hypertension (AH), impacting renal function. Studies have shown that hypertension-induced renal damage involves changes in capillary density (CD), indicating alterations in vascularization. We aimed to elucidate the role of the apelin receptor (APLNR), neuronal nitric oxide synthase (nNOS), and vascular endothelial growth factor (VEGF) in hypertension-induced renal damage. We used two groups of spontaneously hypertensive rats aged 6 and 12 months, representing different stages of AH, and compared them to age-matched normotensive controls. The kidney tissue samples were prepared through a well-established protocol. All data analysis was conducted with a dedicated software program. APLNR was localized in tubular epithelial cells and the endothelial cells of the glomeruli, with higher expression in older SHRs. The localization of nNOS and VEGF was similar. The expression of APLNR and nNOS increased with AH progression, while VEGF levels decreased. CD was lower in young SHRs compared to controls and decreased significantly in older SHRs in comparison to age-matched controls. Our statistical analysis revealed significant differences in molecule expression between age groups and varying correlations between the expression of the three molecules and CD.
Recent Advances in Bacterial Detection Using Surface-Enhanced Raman Scattering
Authors: Hassan Manal , Zhao Yiping , Zughaier Susu M.
Published: August 2024

Rapid identification of microorganisms with a high sensitivity and selectivity is of great interest in many fields, primarily in clinical diagnosis, environmental monitoring, and the food industry. For over the past decades, a surface-enhanced Raman scattering (SERS)-based detection platform has been extensively used for bacterial detection, and the effort has been extended to clinical, environmental, and food samples. In contrast to other approaches, such as enzyme-linked immunosorbent assays and polymerase chain reaction, SERS exhibits outstanding advantages of rapid detection, being culture-free, low cost, high sensitivity, and lack of water interference. This review aims to cover the development of SERS-based methods for bacterial detection with an emphasis on the source of the signal, techniques used to improve the limit of detection and specificity, and the application of SERS in high-throughput settings and complex samples. The challenges and advancements with the implementation of artificial intelligence (AI) are also discussed.
A Combination of Astragaloside IV and Hydroxysafflor Yellow A Attenuates Cerebral Ischemia-Reperfusion Injury via NF-κB/NLRP3/Caspase-1/GSDMD Pathway
Authors: Hou Yongchun , Yan Zi , Wan Haitong , Yang Jiehong , Ding Zhishan , He Yu
Published: August 2024

Cerebral ischemia-reperfusion injury (IRI), occurring after blood supply restoration, contributes significantly to stroke-related deaths. This study explored the combined impact and mechanisms of astragaloside IV (AS-IV), hydroxysafflor yellow A (HSYA), and their combination in mitigating IRI. Male Sprague–Dawley (SD) rats were randomized to the Sham, MCAO, MCAO+AS-IV, MCAO+HSYA, and MCAO+AS-IV+HSYA groups. Neurological deficits and cerebral infarction were examined after restoring the blood supply to the brain. Pathomorphological changes in the cerebral cortex were observed via HE staining. IL-1β and IL-18 were quantified using ELISA. The expression of NF-κB and GSDMD in the ischemic cerebrum was analyzed using immunohistochemistry. The expression levels of NLRP3, ASC, IL-1β, Caspase-1, and GSDMD in the ischemic cerebrum were evaluated using Western blot. The MCAO+AS-IV, MCAO+HSYA, and MCAO+AS-IV+HSYA groups exhibited notably better neurological function and cerebral infarction compared with the MCAO group. The combined treatment demonstrated superior brain tissue injury alleviation. Reductions in NF-κB, GSDMD positive cells, and NLRP3/ASC/IL-1β/Caspase-1/GSDMD protein expression in the ischemic brain were significantly more pronounced with the combined therapy, indicating a synergistic effect in countering cerebral IRI via the NF-κB/NLRP3/Caspase-1/GSDMD pathway inhibition of cell pyroptosis-induced injury.
Going Underground: How Perceived High-Performance Work Systems Influence Bootlegging Behavior? A Multi-Level Moderated Mediation Approach
Authors: Farooqi Asaad Salam , Song Dian , Yin Yishuai , Yuan Yongzhi
Published: August 2024

This study applies the social information processing theory to investigate how perceived high-performance work systems (HPWS) influence bootlegging behavior. Additionally, it explores the potential mediating role of willingness to take risks and creative self-efficacy in the association between perceived HPWS and bootlegging behavior. In addition, this study examines how human resource management (HRM) system strength acts as a cross-level moderator in the connection between perceived HPWS and willingness to take risks and creative self-efficacy. Based on a sample of 399 respondents from 80 firms, the results indicate a positive connection between perceived HPWS and bootlegging behavior. Moreover, the results reveal that willingness to take risks and creative self-efficacy mediate the connection between perceived HPWS and bootlegging behavior. Findings also reveal that cross-level HRM system strength moderates the influence of perceived HPWS on willingness to take risks and creative self-efficacy. The study also highlights its theoretical contributions and practical implications and proposes avenues for future research.
A New Way of Analysing Dreams on Its Profoundest Level: The Development of Motif Analysis and Phase Model (MAP) as an Extension of Structural Dream Analysis (SDA)
Authors: Jenni Patrick , Roesler Christian
Published: August 2024

Dream research today assumes that there is a connection between dreams and waking life. However, the structural alteration of dream motifs in connection with the psychotherapeutic process and waking life has not yet been researched extensively. This study depicts the development of the new Motif Analysis and Phase Model (MAP), a dynamic method which allows research on the previous aspects. The following question was investigated as an accompanying key issue: can a connection be established between the course of the dream patterns and the agency of the dream ego as well as the dream contents and the course of the psychotherapies of the dreaming person as a whole? Four hypotheses were formulated and tested. The data material consists of 217 dreams of a male test subject. The motifs were analysed using Structural Dream Analysis (SDA) at first. Thereafter, the content was linked to the test subject’s waking life in a guided interview. The findings show a strong connection between the dream content and the psychotherapies as well as the test subject’s waking life. Five motifs with structural changes were found, through which the Phase Model with four phases was developed. At turning points, the transformative child motif also appears in the dreams. The course of the dream patterns and agency of the dream ego, however, has not changed. The results, the method and the generalisability were critically discussed and recommendations for future research were formulated.
The Relationships of Self-Sustained English Learning, Language Mindset, Intercultural Communicative Skills, and Positive L2 Self: A Structural Equation Modeling Mediation Analysis
Authors: Yang Luxi , Wang Hui , Zhang Hao , Long Haiying
Published: August 2024

Learning English as a second language (ESL) has garnered significant attention from researchers and practitioners over the past few decades, with numerous ESL learning outcomes examined in the literature. However, self-sustained learning (SSL), a crucial factor in promoting students’ sustained learning and development within a sustainable society, has long been overlooked. To deepen the understanding of SSL, especially in the context of ESL in China, this study examined the direct and indirect effects of intercultural communicative skills, language mindset, and positive L2 self on sustained English learning among 1238 Chinese college students through descriptive statistics and a Structural Equation Modeling (SEM) mediation analysis. The results indicated that Chinese college students exhibited a strong language mindset and positive L2 self. They also scored high in intercultural communicative skills and long-term self-sustained English learning. SEM analysis showed that, after controlling for students’ demographic characteristics, both intercultural communicative skills and language mindset positively predicted positive L2 self and self-sustained English learning. Moreover, intercultural communicative skills and language mindset had significant and positive indirect effects on self-sustained English learning through positive L2 self, underscoring the significant mediating role of positive L2 self in the relationships between intercultural communicative skills, language mindset, and self-sustained English learning. These findings suggest that, to promote self-sustained learning among English learners, instructors should enhance students’ intercultural communicative skills, foster a growth language mindset, and cultivate positive language learning beliefs.
Connections between Parental Emotion Socialization and Internalizing Problems in Adolescents: Examining the Mediating Role of Emotion Regulation Strategies and Moderating Effect of Gender
Authors: Guo Xiaowei , Jiao Ruichao , Wang Jingxin
Published: August 2024

To explore the mediating effect of emotion regulation strategies on the correlation between parental emotion socialization and internalizing problems in adolescents, as well as the moderating effect of gender, a questionnaire survey was administered to 1078 junior high school students (Mage = 13.96 ± 1.00). The results revealed that supportive parental emotion socialization was negatively correlated with adolescents internalizing problems, whereas non-supportive parental emotion socialization was positively correlated with such problems. Cognitive reappraisal and expressive suppression strategies functioned as parallel mediators in the relationship between supportive parental emotion socialization and adolescent internalizing problems, while only expressive suppression mediated the correlation between non-supportive emotion socialization and adolescent internalizing problems. Gender did not exhibit a moderating effect on the mediation model. These findings suggest that supportive parental responses to adolescents’ negative emotions can reduce the incidence of depression and anxiety by cultivating increased utilization of cognitive reappraisal strategies and decreased reliance on expressive suppression strategies among adolescents, whereas non-supportive responses exacerbate the occurrence of depression and anxiety by promoting greater utilization of expressive suppression strategies. In addition, no significant gender differences were observed in the mediation effects. These findings emphasize the importance of prevention programs focusing on parental emotion socialization in adolescence.
Shyness, Sport Engagement, and Internalizing Problems in Chinese Children: The Moderating Role of Class Sport Participation in a Multi-Level Model
Authors: Zhao Rumei , Kong Xiaoxue , Li Mingxin , Zhu Xinyi , Wang Jiyueyi , Ding Wan , Ding Xuechen
Published: August 2024

The relations between shyness and internalizing problems have been mainly explored at the individual level, with little known about its dynamics at the group level. This study aims to examine the mediating effect of individual-level sport engagement and the moderating effect of class-level sport participation in the relations between shyness and internalizing problems. The participants were 951 children attending primary and middle school from grade 3 to grade 7 (Mage = 11 years, 509 boys) in urban areas of China. Cross-sectional data were collected using self-report assessments. Multi-level analysis indicated that (1) shyness was positively associated with internalizing problems; (2) sport engagement partially mediated the relations between shyness and internalizing problems; and (3) class sport participation was a cross-level moderator in the mediating relations between shyness, sport engagement, and internalizing problems. Shy children in classes with a higher level of sport participation tend to have less sport engagement and more internalizing problems than those in classes with a lower level of sport participation. These findings illuminate implications from a multi-level perspective for shy children’s adjustment in a Chinese context. The well-being of shy children could be improved by intervening in sport activity, addressing both individual engagement and group dynamics, such as class participation.
The Impact of Research Pressure on Depression Tendency among Chinese Doctoral Students: The Mediating Effect of Familial Financial Support
Authors: Xu Xiaoqing , Song Guandong , Xiao Bin , Lin Shuangjia
Published: August 2024

Objective: This study aims to explore the impact of research pressure on depression tendency among Chinese doctoral students and analyze the mediating effect of familial financial support in this relationship. Methods: Based on the ecological systems theory, this study employs a mediating effect model and OLS regression model for empirical analysis. Through an online questionnaire, 2815 valid data from Chinese doctoral students were successfully collected. Conclusion: The study finds that research pressure has a significant positive impact on depression tendency among doctoral students (t = 18.347, p < 0.01). Married doctoral students show relatively lower depression tendency, indicating a negative impact of marital status on depression tendency (t = 12.579, p < 0.01). In terms of gender, female doctoral students are more prone to depression compared to their male counterparts (t = −2.921, p < 0.01). Additionally, as the doctoral year progresses, depression tendency also tends to increase (t = 3.690, p < 0.01). Importantly, familial financial support is proven to be a significant mediator between research pressure and depression tendency, explaining 32.116% of the relationship. Suggestion: This study not only provides a multi-dimensional perspective for understanding the mental health issues of doctoral students but also offers a scientific basis for universities and related educational departments to formulate more precise mental health intervention strategies.
Internal Strengths for Adverse Life Events
Authors: Zhao Jian , Chapman Elaine , Houghton Stephen
Published: August 2024

In this study, a brief measure of four internal attributes found to protect against the impact of adverse life events on mental health was developed and validated. In addition, profiles of internal strengths that significantly predict mental health outcomes in young Chinese adults were identified. The results of exploratory and confirmatory factor analyses on data from 831 Chinese university students supported the proposed four-factor model of the ISALES. Participants in the current study fell into two clusters, with one cluster being higher than the other in all four internal strengths, with the former cluster demonstrating better overall mental health than those in the latter cluster. The ISALES is a promising instrument for use in clinical settings and may be used to identify individuals who are more ‘at risk’ of developing poor mental health in the aftermath of a negative life event. The use of the ISALES may permit tailored interventions and timely support to be provided to individuals within clinical settings.
Scale to Evaluate Employee Experience: Evidence of Validity and Reliability in Regular Basic Education Teachers in the Peruvian Context
Authors: Acuña-Hurtado Nilton , García-Salirrosas Elizabeth Emperatriz , Villar-Guevara Miluska , Fernández-Mallma Israel
Published: August 2024

Research on employee experience is a topic that has been growing in recent decades. This study analyzes the validity and reliability of an employee experience scale in Peruvian teachers. The study had an instrumental design. The sample was comprised of 760 Peruvian teachers between 20 and 71 years old (M = 40.91; SD = 10.05), where men (36.1%) and women (63.9%) participated, recruited through non-probabilistic sampling. A validity and reliability analysis of the employee experience scale confirmed the three original factors (sensory experience, intellectual experience, and emotional experience). The KMO test reaches a high level (0.950 > 0.70), and the Bartlett test reaches a highly significant level (Sig. = 0.000). The scale also showed good internal consistency (α = 0.948 to 0.980; CR = 0.950 to 0.981; AVE = 0.864 to 0.878). Similarly, for the confirmatory factor analysis, a measurement adjustment was performed, obtaining excellent and acceptable fit indices for Model 2 for three factors (CMIN/DF = 4.764; CFI = 0.984; SRMR = 0.024; RMSEA = 0.070). This study provides a useful tool to measure the employee experience in a friendly way, using simple language to be applied to the Peruvian context. This study is considered an important contribution to organizational behavior and human talent management in educational circles.