bluuebunny/crossref_metadata_2025 huggingface dataset:
EMG-Assisted Algorithm to Account for Shoulder Muscles Co-Contraction in Overhead Manual Handling
Authors: Assila Najoua , Pizzolato Claudio , Martinez Romain , Lloyd David G. , Begon Mickaël
Published: May 2020

Glenohumeral stability is essential for a healthy function of the shoulder. It is ensured partly by the scapulohumeral muscular balance. Accordingly, modelling muscle interactions is a key factor in the understanding of occupational pathologies, and the development of ergonomic interventions. While static optimization is commonly used to estimate muscle activations, it tends to underestimate the role of shoulder’s antagonist muscles. The purpose of this study was to implement experimental electromyographic (EMG) data to predict muscle activations that could account for the stabilizing role of the shoulder muscles. Kinematics and EMG were recorded from 36 participants while lifting a box from hip to eye level. Muscle activations and glenohumeral joint reactions were estimated using an EMG-assisted algorithm and compared to those obtained using static optimization with a generic and calibrated model. Muscle activations predicted with the EMG-assisted method were generally larger. Additionally, more interactions between the different rotator cuff muscles, as well as between primer actuators and stabilizers, were predicted with the EMG-assisted method. Finally, glenohumeral forces calculated from a calibrated model remained within the boundaries of the glenoid stability cone. These findings suggest that EMG-assisted methods could account for scapulohumeral muscle co-contraction, and thus their contribution to the glenohumeral stability.
Design of the Input and Output Filter for a Matrix Converter Using Evolutionary Techniques
Authors: Muñoz-Castillo Joel , Muñoz-Hernández Germán Ardul , Portilla-Flores Edgar Alfredo , Vega-Alvarado Eduardo , Calva-Yáñez Maria Bárbara , Mino-Aguilar Gerardo , Niño-Suarez Paola Andrea
Published: May 2020

This work presents a new methodology for designing input and output filters to be applied in a three-phase matrix converter, with an optimization problem solved by a metaheuristic algorithm. The algorithm determines the optimal values of the passive elements of the filters, taking as the fundamental performance criterion the response in magnitude. It is based on the resonant frequency, in order to eliminate the harmonics in the input currents and the output voltages produced by the bidirectional switching and disturbances in the power supply. These filters are implemented in a direct matrix converter using a Venturini-type modulation in open loop configuration. Other developments for this filter design have been carried out, but most of them were based on the conventional procedure of determining a random value for some element and then finding the magnitude of the remaining elements. That approach does not ensure the best dynamic filter response. The proposed design technique requires no initial elements, since all values are calculated by the metaheuristic algorithm.
Computation of Stray Losses in Transformer Bushing Regions Considering Harmonics in the Load Current
Authors: Khan Sohail , Maximov Serguei , Escarela-Perez Rafael , Olivares-Galvan Juan Carlos , Melgoza-Vazquez Enrique , Lopez-Garcia Irvin
Published: May 2020

The presence of harmonics in the load current considerably increases stray losses in electric transformers. In this research paper, a new model for computing the electromagnetic field (EMF) and eddy current (EC) losses in transformer tank covers is derived considering harmonics. Maxwell’s equations are solved with their corresponding boundary conditions. The differential equation thus obtained is solved using the method of separation of variables. The obtained expressions do not require the use of special functions, accommodating them for practical implementation in the industry. The obtained formulas are evaluated for different spectrum contents of the load current and losses. The results are in good agreement with simulations carried out using the Altair Flux finite element (FE) software.
Performance of Tilting Pad Journal Bearings with the Same Sommerfeld Number
Authors: Jeung Sung-Hwa , Suh Junho , Yoon Hyun Sik
Published: May 2020

This paper presents the change of non-dimensional characteristics and thermal behavior of different sized tilting pad journal bearings (TPJBs) with the same Sommerfeld number. A three-dimensional (3D) TPJB numerical model is provided considering the thermo-elastic hydro-dynamic (TEHD) lubrication model with pad thermal-elastic deformation. The pivot stiffness is assumed to be the combination of linear and cubic stiffness based on the Hertzian contact theory. The TPJBs in a configuration of load between pad (LBP) with the same Sommerfeld number having seven different sizes are simulated, and their non-dimensional dynamic and static characteristics and thermal behavior are compared. Pad thermal and elastic deformation are both taken into account. If the changes in lubricant viscosity, thermal deformation, and elastic deformation of journal/pads due to viscous shearing are ignored, the bearings with identical Sommerfeld numbers should show the same performance characteristics. However, the heat generation at the bearing clearance during operation (a) induces a decrease in viscosity and heat transfer to journal/pads and (b) results in a thermal deformation. Furthermore, the elastic deformation of the tilting pads and pivots also affects the bearing dynamic performance. For the same Sommerfeld number, the numerical analyses provide how the viscous shearing and elastic deformation lead to a change in bearing performance. For the small bearings with the same Sommerfeld number, the non-dimensional characteristics did not change significantly, where the heat generation was small being compared to the large sized bearing. The largest change in non-dimensional characteristics occurred when the maximum temperature of the oil film increased by 30 °C or more compared to the lubricant supply temperature. The root cause of the change in the non-dimensional characteristics is the viscous shearing in the oil film, and the thermal deformation of the structures surrounding the oil film acts in combination. These results provide insight into the Sommerfeld number, which can be used for the early stage of bearing design.
Effects of Atmospheric-Pressure Cold Plasma Treatment on Deoxynivalenol Degradation, Quality Parameters, and Germination of Barley Grains
Authors: Feizollahi Ehsan , Iqdiam Basheer , Vasanthan Thava , Thilakarathna Malinda S. , Roopesh M. S.
Published: May 2020

Deoxynivalenol (DON) is one of the major trichothecene mycotoxins commonly found in grains, in particular barley. This study focused on the reduction of DON concentration on barley samples using atmospheric cold plasma (ACP) treatment. The effects of moisture content, post-treatment storage, and relative humidity of air on DON degradation on barley were evaluated. Additionally, the germination and the quality parameters of barley, including protein content, β-glucan, and moisture content, were evaluated. The results showed that ACP treatment for 6 and 10 min reduced DON concentration by 48.9% and 54.4%, respectively. No significant differences were observed in the DON degradation levels by increasing the moisture content of barley from 9.5 to 15.7 g water/100 g sample and relative humidity of air from 12 to 60%. Steeping of barley grains without subsequent drying prior to ACP treatment significantly increased the degradation rate of DON by ACP due to the presence of water on the grain surface. No significant differences were observed for the tested quality parameters of barley in comparison with control samples. This study shows that ACP may offer an effective DON reduction in barley without affecting the quality attributes. However, ACP treatment parameters should be optimized to achieve a better DON reduction efficacy.
Brain-Inspired Healthcare Smart System Based on Perception-Action Cycle
Authors: Moreno Escobar Jesús Jaime , Morales Matamoros Oswaldo , Tejeida Padilla Ricardo , Lina Reyes Ixchel , Chanona Hernández Liliana , Ramírez Gutiérrez Ana Gabriela
Published: May 2020

This work presents the HSS-Cognitive project, which is a Healthcare Smart System that can be applied in measuring the efficiency of any therapy where neuronal interaction gives a trace whether the therapy is efficient or not, using mathematical tools. The artificial intelligence of the project underlies in the understanding of brain signals or Electroencephalogram (EEG) by means of the determination of the Power Spectral Density (PSD) over all the EEG bands in order to estimate how efficient was a therapy. Our project HSS-Cognitive was applied, recording the EEG signals from two patients treated for 8 min in a dolphin tank, measuring their activity in five experiments and for 6 min measuring their activity in a pool without dolphin in four experiments. After applying our TEA (Therapeutic Efficiency Assessment) metric for patient 1, we found that this patient had gone from having relaxation states regardless of the dolphin to attention states when the dolphin was presented. For patient 2, we found that he had maintained attention states regardless of the dolphin, that is, the DAT (Dolphin Assisted Therapy) did not have a significant effect in this patient, perhaps because he had a surgery last year in order to remove a tumor, having impact on the DAT effectiveness. However, patient 2 presented the best efficiency when doing physical therapy led by a therapist in a pool without dolphins around him. According to our findings, we concluded that our Brain-Inspired Healthcare Smart System can be considered a reliable tool for measuring the efficiency of a dolphin-assisted therapy and not only for therapist or medical doctors but also for researchers in neurosciences.
A Combined Use of TRIZ Methodology and Eco-Compass tool as a Sustainable Innovation Model
Authors: Boavida Rodrigo , Navas Helena , Godina Radu , Carvalho Helena , Hasegawa Hiroshi
Published: May 2020

In recent years, there has been an increase in the adoption of quality tools by companies. As such, there has been a commitment to innovation by the organizations to obtain competitive advantages by the development of new products and technologies focused on the creation of economic value but also on delivering sustainability. This study aims to develop an application model of the inventive resolution theory in conjunction with the Eco-Compass ecological innovation tool, in order to allow solutions to be obtained systematically, and to present a performance increase of certain environmental parameters, promoting thus sustainable innovation. The case study research methodology is used to frame the research. The company under study is Nokia enterprise, located in Portugal, which offers a set of services related to telecommunications infrastructures. The unit of analysis is the department of transformation and continuous improvement, and the study illustrated the application of combined use of theory of inventive problem solving (TRIZ) and Eco-compass to develop innovative solutions systematically. The results show that it is possible to achieve innovation according to a certain level of established sustainable environmental parameters, while at the same time solving the identified inventive problem.
Special Issue on Body Area Networks
Authors: Pereira António , Costa Nuno , Fernández-Caballero Antonio
Published: May 2020

Wireless body area networks (WBANs) are a fascinating research field offering wearable and implantable sensor technology [...]
Semi-Supervised Speech Recognition Acoustic Model Training Using Policy Gradient
Authors: Chung Hoon , Lee Sung Joo , Jeon Hyeong Bae , Park Jeon Gue
Published: May 2020

In this paper, we propose a policy gradient-based semi-supervised speech recognition acoustic model training. In practice, self-training and teacher/student learning are one of the widely used semi-supervised training methods due to their scalability and effectiveness. These methods are based on generating pseudo labels for unlabeled samples using a pre-trained model and selecting reliable samples using confidence measure. However, there are some considerations in this approach. The generated pseudo labels can be biased depending on which pre-trained model is used, and the training process can be complicated because the confidence measure is usually carried out in post-processing using external knowledge. Therefore, to address these issues, we propose a policy gradient method-based approach. Policy gradient is a reinforcement learning algorithm to find an optimal behavior strategy for an agent to obtain optimal rewards. The policy gradient-based approach provides a framework for exploring unlabeled data as well as exploiting labeled data, and it also provides a way to incorporate external knowledge in the same training cycle. The proposed approach was evaluated on an in-house non-native Korean recognition domain. The experimental results show that the method is effective in semi-supervised acoustic model training.
A Hierarchical Control System for Autonomous Driving towards Urban Challenges
Authors: Van Nam Dinh , Sualeh Muhammad , Kim Dohyeong , Kim Gon-Woo
Published: May 2020

In recent years, the self-driving car technologies have been developed with many successful stories in both academia and industry. The challenge for autonomous vehicles is the requirement of operating accurately and robustly in the urban environment. This paper focuses on how to efficiently solve the hierarchical control system of a self-driving car into practice. This technique is composed of decision making, local path planning and control. An ego vehicle is navigated by global path planning with the aid of a High Definition map. Firstly, we propose the decision making for motion planning by applying a two-stage Finite State Machine to manipulate mission planning and control states. Furthermore, we implement a real-time hybrid A* algorithm with an occupancy grid map to find an efficient route for obstacle avoidance. Secondly, the local path planning is conducted to generate a safe and comfortable trajectory in unstructured scenarios. Herein, we solve an optimization problem with nonlinear constraints to optimize the sum of jerks for a smooth drive. In addition, controllers are designed by using the pure pursuit algorithm and the scheduled feedforward PI controller for lateral and longitudinal direction, respectively. The experimental results show that the proposed framework can operate efficiently in the urban scenario.
Ultrasonic Technology Applied against Mosquito Larvae
Authors: Kalimuthu Kandasamy , Tseng Li-Chun , Murugan Kadarkarai , Panneerselvam Chellasamy , Aziz Al Thabiani , Benelli Giovanni , Hwang Jiang-Shiou
Published: May 2020

The effective management of mosquito vectors is a timely challenge for medical and veterinary entomology. In this study, we evaluated the acoustic Larvasonic device to control young instars of the mosquito Aedes aegypti in diverse freshwater environments. Under laboratory conditions, we investigated the effect of exposure time and distance from the transducer on the mortality of larvae and pupae of Ae. aegypti. Furthermore, we evaluated the effectiveness of the ultrasound window of the electromagnetic spectrum under different field conditions. Results showed that first and second instar larvae were more sensitive to the frequency range of 18–30 kHz of the Larvasonic device. Ultrasonic waves applied for 180 s at a frequency from 18 to 30 kHz caused 100% larval mortality at a distance of 60 cm from the transducer. No mortality was observed in the non-target copepod Megacyclops formosanus. The exposure to the soundwaves produced by the acoustic larvicidal device over different distances effectively damaged Ae. aegypti through destruction of the larval dorsal tracheal trunk, thorax and abdomen. Overall, results indicated that the Larvasonic device tested can provide an alternative tool to reduce young instar populations of Ae. aegypti, without any effects on non-target aquatic invertebrates like copepods. It turned out to be a useful device for mosquito biocontrol. This technology has a relevant potential to fight the spread of mosquito-borne diseases.
Axial Vibration Characteristics of Fluid-Structure Interaction of an Aircraft Hydraulic Pipe Based on Modified Friction Coupling Model
Authors: Quan Lingxiao , Che Shichao , Guo Changhong , Gao Haihai , Guo Meng
Published: May 2020

This paper aims at studying the axial vibration response characteristics of Fluid-Structure Interaction (FSI) vibration of aircraft hydraulic pipe when considering the friction coupling. Based on the Brunone empirical model and the Zielke weighting function, an expression of fluid shear stress of the hydraulic pipeline is presented for a wide range of Reynolds number, and the friction model of the FSI 14-equation for high-speed and high-pressure hydraulic pipeline is modified. On this basis, a left-wing hydraulic pipeline of the C919 airplane is taken as the verification object and modeled, then the FSI vibration 14-equation is solved using frequency-domain transfer matrix method in MATLAB to analyze the modal and the axial vibration characteristics of the pipeline. Ultimately, a test experiment is given and discussed by being compared with the numerical simulation results, which confirmed the correctness of the friction model and demonstrated that the analytic accuracy of axial velocity response of FSI vibration could be improved by considering the friction coupling.
The Torah Ark of Arthur Szyk
Authors: Nashman Fraiman Susan
Published: May 2020

This paper discusses the design and symbolism of a hitherto unpublished work by the artist Arthur Szyk (1894–1951), an ark for the Torah which he designed for the Forest Hills Jewish Center of Queens, New York, and which was dedicated in 1949. The Torah Ark is the central focus of all synagogue worship. Szyk’s ark is unique in its multiplicity of symbols and texts, which was at odds with the modernist idiom of post-World War II synagogue architecture. This research, which also brings previously unpublished material, analyzes the possible sources for the work and its distinctive message, which is exceptional in the world of modern contemporary Jewish art.
Assessment of Air Quality in School Environments in Hanoi, Vietnam: A Focus on Mass-Size Distribution and Elemental Composition of Indoor-Outdoor Ultrafine/Fine/Coarse Particles
Authors: Tran Trinh Dinh , Nguyen Phuong Minh , Nghiem Dung Trung , Le Tuyen Huu , Tu Minh Binh , Alleman Laurent Y. , Nguyen Viet Minh , Pham Dong Thanh , Ha Ngoc Minh , Dang Minh Nhat , Le Chieu Van , Nguyen Noi Van
Published: May 2020

Indoor and outdoor ultrafine, accumulation mode, and coarse fractions collected at two preschools (S1 and S2) in Hanoi capital, Vietnam were characterized in terms of mass-size distribution and elemental composition to identify major emission sources. The sampling campaigns were performed simultaneously indoors and outdoors over four consecutive weeks at each school. Indoor average concentrations of CO2 and CO at both schools were below the limit values recommended by American Society of Heating, Refrigerating and Air-Conditioning Engineers (1000 ppm for CO2) and World Health Organization (7 mg/m3 for CO). Indoor concentrations of PM2.5 and PM10 at S1 and S2 were strongly influenced by the presence of children and their activities indoors. The indoor average concentrations of PM2.5 and PM10 were 49.4 µg/m3 and 59.7 µg/m3 at S1, while those values at S2 were 7.9 and 10.8 µg/m3, respectively. Mass-size distribution of indoor and outdoor particles presented similar patterns, in which ultrafine particles accounted for around 15–20% wt/wt while fine particles (PM2.5) made up almost 80% wt/wt of PM10. PM2.5–10 did not display regular shapes while smaller factions tended to aggregate to form clusters with fine structures. Oxygen (O) was the most abundant element in all fractions, followed by carbon (C) for indoor and outdoor particles. O accounted for 36.2% (PM0.5–1) to 42.4% wt/wt (PM0.1) of indoor particles, while those figures for C were in the range of 14.5% (for PM0.1) to 18.1% (for PM1–2.5). Apart from O and C, mass proportion of other major and minor elements (Al, Ca, Cr, Fe , K, Mg, Si, Ti) could make up to 50%, whereas trace elements (As, Bi, Cd, Co, Cr, Cu, La, Mn, Mo, Ni, Pb, Rb, Sb, Se, Sn, Sr, and Zn) accounted for less than 0.5% of indoor and outdoor airborne particles. There were no significant indoor emission sources of trace and minor elements. Traffic significantly contributed to major and trace elements at S1 and S2.
Nanoparticle Number Concentration in the Air in Relation to the Time of the Year and Time of the Day
Authors: Brzezina Jáchym , Köbölová Klaudia , Adamec Vladimír
Published: May 2020

The paper analyzes suspended particles number concentrations of 61 size fractions (184 nm to 17,165 nm) in the air at a traffic location. The average course of the individual fractions was analyzed at various intervals – daily, weekly, monthly and annually, in the period between 2017 and 2019. The data was then used to calculate the arithmetic mean for all the fractions (MS Excel, R) and then using a proprietary web application, heatmaps were constructed. The obtained results showed significant differences in both the annual and daily variation of number concentrations between the individual fractions differing in particle size. In the case of the annual variation, one can see a greater variability of smaller particles, which is most likely due to the source of the actual suspended particles. Meteorological and dispersion conditions are found as important factors for suspended particle concentrations. These can lead to significant differences from year to year. However, a comparison between 2018 and 2019 showed that even though the average absolute number concentrations can differ between years, the actual relative number concentrations, i.e., the ratios between the individual fractions remain very similar. In conclusion it can be said that the difference between the number concentration variation of the size fractions depends on both the actual pollution sources (especially in the long-term, i.e., the annual variation) and the actual size of the particles, which plays a role especially in the short-term (daily, weekly variation).
Period of Boar Ejaculate Collection Contributes to the Yearly Intra-Male Variability of Seminal Plasma Cytokines
Authors: Padilla Lorena , Lucas Xiomara , Parrilla Inmaculada , Perez-Patiño Cristina , Rodriguez-Martinez Heriberto , Roca Jordi , Barranco Isabel
Published: May 2020

The concentrations of cytokines in seminal plasma (SP) fluctuate over time in healthy males, weakening their practical usefulness as diagnostic tools. This study evaluated the relevance of intra-male variability in SP cytokines and to what extent the period of the year when ejaculate is collected contributes to such variability. Thirteen cytokines (GM-CSF, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18, and TNFα) were measured using the Luminex xMAP® technology for 180 SP samples of ejaculate collected over a year from nine healthy and fertile boars. The SP samples were grouped into two annual periods according to decreasing or increasing daylight and ambient temperature. Intra-male variability was higher than inter-male variability for all cytokines. All SP cytokines showed concentration differences between the two periods of the year, showing the highest concentration during the increasing daylength/temperature period, irrespective of the male. Similarly, some cytokines showed differences between daylength/temperature periods when focusing on their total amount in the ejaculate. No strong relationship (explaining more than 50% of the total variance) was found between annual fluctuations in SP-cytokine levels and semen parameters. In conclusion, the period of the year during which ejaculates were collected helps explain the intra-male variability of SP-cytokine levels in breeding boars.
The NRF2/KEAP1 Axis in the Regulation of Tumor Metabolism: Mechanisms and Therapeutic Perspectives
Authors: Panieri Emiliano , Telkoparan-Akillilar Pelin , Suzen Sibel , Saso Luciano
Published: May 2020

The NRF2/KEAP1 pathway is a fundamental signaling cascade that controls multiple cytoprotective responses through the induction of a complex transcriptional program that ultimately renders cancer cells resistant to oxidative, metabolic and therapeutic stress. Interestingly, accumulating evidence in recent years has indicated that metabolic reprogramming is closely interrelated with the regulation of redox homeostasis, suggesting that the disruption of NRF2 signaling might represent a valid therapeutic strategy against a variety of solid and hematologic cancers. These aspects will be the focus of the present review.
Effects of the Positive Allosteric Modulator of Metabotropic Glutamate Receptor 5, VU-29, on Maintenance Association between Environmental Cues and Rewarding Properties of Ethanol in Rats
Authors: Marszalek-Grabska Marta , Gawel Kinga , Matosiuk Dariusz , Gibula-Tarlowska Ewa , Listos Joanna , Kotlinska Jolanta H.
Published: May 2020

Metabotropic glutamate subtype 5 (mGlu5) receptors are implicated in various forms of synaptic plasticity, including drugs of abuse. In drug-addicted individuals, associative memories can drive relapse to drug use. The present study investigated the potential of the mGlu5 receptor positive allosteric modulator (PAM), VU-29 (30 mg/kg, i.p.), to inhibit the maintenance of a learned association between ethanol and environmental context by using conditioned place preference (CPP) in rats. The ethanol-CPP was established by the administration of ethanol (1.0 g/kg, i.p. ×10 days) using an unbiased procedure. Following ethanol conditioning, VU-29 was administered at various post-conditioning times (ethanol free state at the home cage) to ascertain if there was a temporal window during which VU-29 would be effective. Our experiments indicated that VU-29 did not affect the expression of ethanol-induced CPP when it was given over two post-conditioning days. However, the expression of ethanol-CPP was inhibited by 10-day home cage administration of VU-29, but not by first 2-day or last 2-day injection of VU-29 during the 10-day period. These findings reveal that VU-29 can inhibit the maintenance of ethanol-induced CPP, and that treatment duration contributes to this effect of VU-29. Furthermore, VU-29 effect was reversed by pretreatment with either MTEP (the mGlu5 receptor antagonist), or MK-801 (the N-methyl-D-aspartate-NMDA receptor antagonist). Thus, the inhibitory effect of VU-29 is dependent on the functional interaction between mGlu5 and NMDA receptors. Because a reduction in ethanol-associated cues can reduce relapse, mGlu5 receptor PAM would be useful for therapy of alcoholism. Future research is required to confirm the current findings.
Organic Cation Transporters (OCTs) in EpiAirway™, a Cellular Model of Normal Human Bronchial Epithelium
Authors: Barilli Amelia , Visigalli Rossana , Ferrari Francesca , Di Lascia Maria , Riccardi Benedetta , Puccini Paola , Dall’Asta Valeria , Rotoli Bianca Maria
Published: May 2020

Organic cation transporters (OCTs) and novel organic cation transporters (OCTNs) are responsible for drug delivery in the intestine and kidney; in the lung, OCTs mediate inhaled drugs’ transport, although their physiological role in airways remains poorly understood. The studies addressing OCTs/OCTNs in human airways were mostly performed in immortal or transformed cell lines; here, we studied OCTs in EpiAirway™, a recently developed in vitro model of normal bronchial epithelium. Calu-3 monolayers were used for comparison. The activity of OCTs was evaluated by measuring the uptake of 1-methyl-4-phenylpyridinium (MPP+) at the apical and basolateral side of monolayers and protein expression through Western Blot analysis. OCTs and OCTNs expression, along with that of Amino acid Transporter B0,+ (ATB0,+)transporter, was determined by measuring the number of mRNA molecules through quantitative Polymerase Chain Reaction (qPCR). The interaction of the transporters with bronchodilators was also assessed. Results highlight significant differences between Calu-3 cells and EpiAirway™, since, in the latter, OCTs are active only on the basolateral membrane where they interact with the bronchodilator ipratropium. No activity of OCTs is detectable at the apical side; there, the most abundant carrier is, instead, SLC6A14/ATB0,+, that can thus be potentially listed among organic cation transporters responsible for drug delivery in the lung.
Characterisation of a Novel A-Superfamily Conotoxin
Authors: Wilson David T. , Bansal Paramjit S. , Carter David A. , Vetter Irina , Nicke Annette , Dutertre Sébastien , Daly Norelle L.
Published: May 2020

Conopeptides belonging to the A-superfamily from the venomous molluscs, Conus, are typically α-conotoxins. The α-conotoxins are of interest as therapeutic leads and pharmacological tools due to their selectivity and potency at nicotinic acetylcholine receptor (nAChR) subtypes. Structurally, the α-conotoxins have a consensus fold containing two conserved disulfide bonds that define the two-loop framework and brace a helical region. Here we report on a novel α-conotoxin Pl168, identified from the transcriptome of Conus planorbis, which has an unusual 4/8 loop framework. Unexpectedly, NMR determination of its three-dimensional structure reveals a new structural type of A-superfamily conotoxins with a different disulfide-stabilized fold, despite containing the conserved cysteine framework and disulfide connectivity of classical α-conotoxins. The peptide did not demonstrate activity on a range of nAChRs, or Ca2+ and Na+ channels suggesting that it might represent a new pharmacological class of conotoxins.
CO2 Conversion to Methanol over Novel Carbon Nanofiber-Based Cu/ZrO2 Catalysts—A Kinetics Study
Authors: Din Israf Ud , Shaharun Maizatul S. , Naeem Abdul , Alotaibi Mshari A. , Alharthi Abdulrahman I. , Nasir Qazi
Published: May 2020

Ongoing industrialization has deteriorated the global environment. Global warming is a human-induced issue affecting the environment. The alarming increase in CO2 emissions is among the major contributors to global warming. The conversion of CO2 to methanol is an economically viable and environmentally friendly solution to mitigate its concentration. Here, hydrogenation of CO2 was studied over carbon nanofiber-based Cu/ZrO2 catalysts. Kinetics investigations were carried out for the reaction. Overall, kinetics data indicated that CO2 conversion follows a pseudo-first-order reaction. The kinetics studies were further modeled by using an artificial neural network, which supported the experimental kinetics study.
Enantioselective Epoxidation by Flavoprotein Monooxygenases Supported by Organic Solvents
Authors: Eggerichs Daniel , Mügge Carolin , Mayweg Julia , Apfel Ulf-Peter , Tischler Dirk
Published: May 2020

Styrene and indole monooxygenases (SMO and IMO) are two-component flavoprotein monooxygenases composed of a nicotinamide adenine dinucleotide (NADH)-dependent flavin adenine dinucleotide (FAD)-reductase (StyB or IndB) and a monooxygenase (StyA or IndA). The latter uses reduced FAD to activate oxygen and to oxygenate the substrate while releasing water. We circumvented the need for the reductase by direct FAD reduction in solution using the NAD(P)H-mimic 1-benzyl-1,4-dihydronicotinamide (BNAH) to fuel monooxygenases without NADH requirement. Herein, we report on the hitherto unknown solvent tolerance for the indole monooxygenase from Gemmobacter nectariphilus DSM15620 (GnIndA) and the styrene monooxygenase from Gordonia rubripertincta CWB2 (GrStyA). These enzymes were shown to convert bulky and rather hydrophobic styrene derivatives in the presence of organic cosolvents. Subsequently, BNAH-driven biotransformation was furthermore optimized with regard to the applied cosolvent and its concentration as well as FAD and BNAH concentration. We herein demonstrate that GnIndA and GrStyA enable selective epoxidations of allylic double bonds (up to 217 mU mg−1) in the presence of organic solvents such as tetrahydrofuran, acetonitrile, or several alcohols. Notably, GnIndA was found to resist methanol concentrations up to 25 vol.%. Furthermore, a diverse substrate preference was determined for both enzymes, making their distinct use very interesting. In general, our results seem representative for many IMOs as was corroborated by in silico mutagenetic studies.
Pd-Catalyzed Intermolecular Dehydrogenative Heck Reactions of Five-Membered Heteroarenes
Authors: Le Bras Jean , Muzart Jacques
Published: May 2020

The Pd-mediated cross-coupling of (hetero)arenes with alkenes may be an effective method for the formation of a C–C bond from two C–H bonds. Discovered by Fujiwara and co-workers in 1967, this reaction led to a number of reports that we firstly highlighted in 2011 (review with references till June 2010) and for which, we retained the name “dehydrogenative Heck reaction”. The topic, especially the reactions of five-membered heteroarenes, has been the subject of intensive research over the last ten years. The present review is limited to these dehydrogenative Heck reactions published since 2010, underlining the progress of the procedures.
Catalytic Applications of CeO2-Based Materials
Authors: Vita Antonio
Published: May 2020

Among the metal-oxides, ceria represents one of the most promising materials for chemical transformations mainly for environmental applications and energy conversion systems [...]
Disease-Specific Changes in Reelin Protein and mRNA in Neurodegenerative Diseases
Authors: Lidón Laia , Urrea Laura , Llorens Franc , Gil Vanessa , Alvarez Ignacio , Diez-Fairen Monica , Aguilar Miguel , Pastor Pau , Zerr Inga , Alcolea Daniel , Lleó Alberto , Vidal Enric , Gavín Rosalina , Ferrer Isidre , Del Rio Jose Antonio
Published: May 2020

Reelin is an extracellular glycoprotein that modulates neuronal function and synaptic plasticity in the adult brain. Decreased levels of Reelin activity have been postulated as a key factor during neurodegeneration in Alzheimer’s disease (AD) and in aging. Thus, changes in levels of full-length Reelin and Reelin fragments have been revealed in cerebrospinal fluid (CSF) and in post-mortem brains samples of AD patients with respect to non-AD patients. However, conflicting studies have reported decreased or unchanged levels of full-length Reelin in AD patients compared to control (nND) cases in post-mortem brains and CSF samples. In addition, a compelling analysis of Reelin levels in neurodegenerative diseases other than AD is missing. In this study, we analyzed brain levels of RELN mRNA and Reelin protein in post-mortem frontal cortex samples from different sporadic AD stages, Parkinson’s disease with dementia (PDD), and Creutzfeldt-Jakob disease (sCJD), obtained from five different Biobanks. In addition, we measured Reelin protein levels in CSF samples of patients with mild cognitive impairment (MCI), dementia, or sCJD diagnosis and a group of neurologically healthy cases. The results indicate an increase in RELN mRNA in the frontal cortex of advanced stages of AD and in sCJD(I) compared to controls. This was not observed in PDD and early AD stages. However, Reelin protein levels in frontal cortex samples were unchanged between nND and advanced AD stages and PDD. Nevertheless, they decreased in the CSF of patients with dementia in comparison to those not suffering with dementia and patients with MCI. With respect to sCJD, there was a tendency to increase in brain samples in comparison to nND and to decrease in the CSF with respect to nND. In conclusion, Reelin levels in CSF cannot be considered as a diagnostic biomarker for AD or PDD. However, we feel that the CSF Reelin changes observed between MCI, patients with dementia, and sCJD might be helpful in generating a biomarker signature in prodromal studies of unidentified dementia and sCJD.
HLA-DPB1 Reactive T Cell Receptors for Adoptive Immunotherapy in Allogeneic Stem Cell Transplantation
Authors: Klobuch Sebastian , Hammon Kathrin , Vatter-Leising Sarah , Neidlinger Elisabeth , Zwerger Michael , Wandel Annika , Neuber Laura Maria , Heilmeier Bernhard , Fichtner Regina , Mirbeth Carina , Herr Wolfgang , Thomas Simone
Published: May 2020

HLA-DPB1 antigens are mismatched in about 80% of allogeneic hematopoietic stem cell transplantations from HLA 10/10 matched unrelated donors and were shown to be associated with a decreased risk of leukemia relapse. We recently developed a reliable in vitro method to generate HLA-DPB1 mismatch-reactive CD4 T-cell clones from allogeneic donors. Here, we isolated HLA-DPB1 specific T cell receptors (TCR DP) and used them either as wild-type or genetically optimized receptors to analyze in detail the reactivity of transduced CD4 and CD8 T cells toward primary AML blasts. While both CD4 and CD8 T cells showed strong AML reactivity in vitro, only CD4 T cells were able to effectively eliminate leukemia blasts in AML engrafted NOD/SCID/IL2Rγc−/− (NSG) mice. Further analysis showed that optimized TCR DP and under some conditions wild-type TCR DP also mediated reactivity to non-hematopoietic cells like fibroblasts or tumor cell lines after HLA-DP upregulation. In conclusion, T cells engineered with selected allo-HLA-DPB1 specific TCRs might be powerful off-the-shelf reagents in allogeneic T-cell therapy of leukemia. However, because of frequent (common) cross-reactivity to non-hematopoietic cells with optimized TCR DP T cells, safety mechanisms are mandatory.
A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping
Authors: Romano Maria , Ruggiero Alessia , Squeglia Flavia , Maga Giovanni , Berisio Rita
Published: May 2020

The current coronavirus disease-2019 (COVID-19) pandemic is due to the novel coronavirus SARS-CoV-2. The scientific community has mounted a strong response by accelerating research and innovation, and has quickly set the foundation for understanding the molecular determinants of the disease for the development of targeted therapeutic interventions. The replication of the viral genome within the infected cells is a key stage of the SARS-CoV-2 life cycle. It is a complex process involving the action of several viral and host proteins in order to perform RNA polymerization, proofreading and final capping. This review provides an update of the structural and functional data on the key actors of the replicatory machinery of SARS-CoV-2, to fill the gaps in the currently available structural data, which is mainly obtained through homology modeling. Moreover, learning from similar viruses, we collect data from the literature to reconstruct the pattern of interactions among the protein actors of the SARS-CoV-2 RNA polymerase machinery. Here, an important role is played by co-factors such as Nsp8 and Nsp10, not only as allosteric activators but also as molecular connectors that hold the entire machinery together to enhance the efficiency of RNA replication.
Tendon and Cytokine Marker Expression by Human Bone Marrow Mesenchymal Stem Cells in a Hyaluronate/Poly-Lactic-Co-Glycolic Acid (PLGA)/Fibrin Three-Dimensional (3D) Scaffold
Authors: Ciardulli Maria C. , Marino Luigi , Lovecchio Joseph , Giordano Emanuele , Forsyth Nicholas R. , Selleri Carmine , Maffulli Nicola , Porta Giovanna Della
Published: May 2020

We developed a (three-dimensional) 3D scaffold, we named HY-FIB, incorporating a force-transmission band of braided hyaluronate embedded in a cell localizing fibrin hydrogel and poly-lactic-co-glycolic acid (PLGA) nanocarriers as transient components for growth factor controlled delivery. The tenogenic supporting capacity of HY-FIB on human-Bone Marrow Mesenchymal Stem Cells (hBM-MSCs) was explored under static conditions and under bioreactor-induced cyclic strain conditions. HY-FIB elasticity enabled to deliver a mean shear stress of 0.09 Pa for 4 h/day. Tendon and cytokine marker expression by hBM-MSCs were studied. Results: hBM-MSCs embedded in HY-FIB and subjected to mechanical stimulation, resulted in a typical tenogenic phenotype, as indicated by type 1 Collagen fiber immunofluorescence. RT-qPCR showed an increase of type 1 Collagen, scleraxis, and decorin gene expression (3-fold, 1600-fold, and 3-fold, respectively, at day 11) in dynamic conditions. Cells also showed pro-inflammatory (IL-6, TNF, IL-12A, IL-1β) and anti-inflammatory (IL-10, TGF-β1) cytokine gene expressions, with a significant increase of anti-inflammatory cytokines in dynamic conditions (IL-10 and TGF-β1 300-fold and 4-fold, respectively, at day 11). Mechanical signaling, conveyed by HY-FIB to hBM-MSCs, promoted tenogenic gene markers expression and a pro-repair cytokine balance. The results provide strong evidence in support of the HY-FIB system and its interaction with cells and its potential for use as a predictive in vitro model.
Comparison of the Clinical Results of Identically Designed Total Knee Prostheses with Different Surface Roughnesses
Authors: Ko Sang Hyun , Moon Kyoung Ho
Published: May 2020

The purpose of this study was to investigate the effects of the undersurface roughness of total knee prosthesis on clinical outcomes. We compared the clinical and radiological outcomes and prosthesis survivals in patients who underwent total knee arthroplasty using prosthesis with identical designs but different surface roughness (average surface roughnesses (Ra), 5.0 μm vs. 11.6 μm). The results showed that the knee prostheses with a more roughened undersurface (Ra = 11.6 μm) produced significantly better functional results and enhanced prosthesis survival. The difference in surface roughness was associated with incidence of osteolysis and loosening at the tibial baseplate, but not at the femoral component. Overall, our results provided significant evidence that the use of roughened undersurface of tibial baseplate would be a way to prevent aseptic loosening.
Magnetocaloric Effect, Magnetoresistance of Sc0.28Ti0.72Fe2, and Phase Diagrams of Sc0.28Ti0.72Fe2−xTx Alloys with T = Mn or Co
Authors: Sun Liting , Yibole Hargen , Tegus Ojiyed , Guillou Francois
Published: May 2020

(Sc,Ti)Fe2 Laves phases present a relatively unique case of first-order ferro-ferromagnetic transition originating from an instability of the Fe moment. In addition to large magnetoelastic effects making them potential negative thermal expansion materials, here, we show that Sc0.28Ti0.72Fe2 and related alloys also present sizable magnetocaloric and magnetoresistance effects. Both effects are found substantially larger at the ferro-ferromagnetic transition (Tt1) than near the Curie temperature TC, yet they remain limited in comparison to other classes of giant magnetocaloric materials. We suggest a strategy to improve these properties by bringing the transition at Tt1 close to TC, and test its possible realization by Co or Mn for Fe substitutions. The structural and magnetic phase diagrams of Sc0.28Ti0.72Fe2−xTx alloys with T = Mn or Co are explored. Substitutions for Fe by adjacent Mn or Co elements give rise to a breakdown of the long-range ferromagnetic order, as well as a swift disappearance of finite moment magnetism.
Atomic Force Microscopy Study of Discrete Dislocation Pile-ups at Grain Boundaries in Bi-Crystalline Micro-Pillars
Authors: Chen Xiaolei , Richeton Thiebaud , Motz Christian , Berbenni Stéphane
Published: May 2020

Compression tests at low strains were performed to theoretically analyze the effects of anisotropic elasticity, misorientation, grain boundary (GB) stiffness, interfacial dislocations, free surfaces, and critical force on dislocation pile-ups in micro-sized Face-Centered Cubic (FCC) Nickel (Ni) and α -Brass bi-crystals. The spatial variations of slip heights due to localized slip bands terminating at GB were measured by Atomic Force Microscopy (AFM) to determine the Burgers vector distributions in the dislocation pile-ups. These distributions were then simulated by discrete pile-up micromechanical calculations in anisotropic bi-crystals consistent with the experimentally measured material parameters. The computations were based on the image decomposition method considering the effects of interphase GB and free surfaces in multilayered materials. For Ni and α -Brass, it was found that the best predicted step height spatial profiles were obtained considering anisotropic elasticity, free surface effects, a homogeneous external stress and a certain critical force in the material to equilibrate the dislocation pile-ups.
Meiofauna Life on Loggerhead Sea Turtles-Diversely Structured Abundance and Biodiversity Hotspots That Challenge the Meiofauna Paradox
Authors: Ingels Jeroen , Valdes Yirina , Pontes Letícia P. , Silva Alexsandra C. , Neres Patrícia F. , Corrêa Gustavo V. V. , Silver-Gorges Ian , Fuentes Mariana M.P.B. , Gillis Anthony , Hooper Lindsay , Ware Matthew , O’Reilly Carrie , Bergman Quintin , Danyuk Julia , Sanchez Zarate Sofia , Acevedo Natale Laura I. , dos Santos Giovanni A. P.
Published: May 2020

Sea turtles migrate thousands of miles annually between foraging and breeding areas, carrying dozens of epibiont species with them on their journeys. Most sea turtle epibiont studies have focused on large-sized organisms, those visible to the naked eye. Here, we report previously undocumented levels of epibiont abundance and biodiversity for loggerhead sea turtles (Caretta caretta), by focusing on the microscopic meiofauna. During the peak of the 2018 loggerhead nesting season at St. George Island, Florida, USA, we sampled all epibionts from 24 carapaces. From the subsamples, we identified 38,874 meiofauna individuals belonging to 20 higher taxa. This means 810,753 individuals were recovered in our survey, with an average of 33,781 individuals per carapace. Of 6992 identified nematodes, 111 different genera were observed. To our knowledge, such levels of sea turtle epibiont abundance and diversity have never been recorded. Loggerhead carapaces are without doubt hotspots of meiofaunal and nematode diversity, especially compared to other non-sedimentary substrates. The posterior carapace sections harbored higher diversity and evenness compared to the anterior and middle sections, suggesting increased colonization and potentially facilitation favoring posterior carapace epibiosis, or increased disturbance on the anterior and middle carapace sections. Our findings also shed new light on the meiofauna paradox: “How do small, benthic meiofauna organisms become cosmopolitan over large geographic ranges?” Considering high loggerhead epibiont colonization, the large distances loggerheads migrate for reproduction and feeding, and the evolutionary age and sheer numbers of sea turtles worldwide, potentially large-scale exchange and dispersal for meiofauna through phoresis is implied. We distinguished different groups of loggerhead carapaces based on divergent epibiont communities, suggesting distinct epibiont colonization processes. These epibiont observations hold potential for investigating loggerhead movements and, hence, their conservation.