bluuebunny/crossref_metadata_2025 huggingface dataset:
One-Pot Hydrothermal Synthesis and Electrochemical Performance of Subspheroidal Core–Shell Structure MoS2/C Composite as Anode Material for Lithium-Ion Batteries
Authors: Liu Wei , Fan Dongsheng , Wang Wei , Yang Shenshen , Lu Yaozong , Fu Lingping , Zhang Jingbo , Wu Yang
Published: April 2024

Molybdenum disulfide (MoS2) is a promising anode material for lithium-ion batteries (LIBs) due to its distinctive graphene-like structure and high specific capacity. However, its commercial application is hindered by the severe volume expansion during lithiation/delithiation and poor conductivity. In this paper, we report a facile one-pot enhanced hydrothermal synthesis strategy to prepare high-performance MoS2/C composite materials. The results indicate that the as-prepared MoS2/C composite is a subspheroidal core–shell structure material, with uniform coating, good particle dispersion, and an average grain size of approximately 80 nm. The morphology of the composite remained unchanged even after annealing at 500 °C for 2 h. The addition of glucose can accelerate the nucleation and growth of MoS2, and higher hydrothermal temperatures can improve the product yield. The addition of PVP has little effect on the yield, but significantly reduces the particle size. The XPS analysis reveals that the MoO3 may be generated as an intermediate product during the hydrothermal process. The electrochemical test results show that the unannealed MoS2/C samples exhibit discharge-specific capacities of 705.2 mAh·g−1 and 625.7 mAh·g−1 after the first cycle and the 100th cycle, respectively, at a current density of 500 mA·g−1, with a capacity retention rate of 88.7%. In contrast, the specific capacity of the MoS2/C specimens after annealing at 500 °C for 2 h shows a tendency to decrease and then slowly increase during the cycles, and the discharge specific capacity is 582.3 mAh·g−1 after the 100th cycle, which is lower than that of the unheated sample. The impedance analysis reveals that the lithium-ion diffusion coefficient of the MoS2/C material without calcination is 2.11 × 10−18 cm·s−2, which is superior to that of the annealed MoS2/C and pristine MoS2 samples. This characteristic is favorable for lithiation/delithiation during the charge/discharge process.
An Adaptive Modeling Method for the Prognostics of Lithium-Ion Batteries on Capacity Degradation and Regeneration
Authors: Deng Liming , Shen Wenjing , Xu Kangkang , Zhang Xuhui
Published: April 2024

Accurate prediction of remaining useful life (RUL) is crucial to the safety and reliability of the lithium-ion battery management system (BMS). However, the performance of a lithium-ion battery deteriorates nonlinearly and is heavily affected by capacity-regeneration phenomena during practical usage, which makes battery RUL prediction challenging. In this paper, a rest-time-based regeneration-phenomena-detection module is proposed and incorporated into the Coulombic efficiency-based degradation model. The model is estimated with the particle filter method to deal with the nonlinear uncertainty during the degradation and regeneration process. The discrete regeneration-detection results should be reflected by the model state instead of the model parameters during the particle filter-estimation process. To decouple the model state and model parameters during the estimation process, a dual-particle filtering estimation framework is proposed to update the model parameters and model state, respectively. A kernel smoothing method is adopted to further smooth the evolution of the model parameters, and the regeneration effects are imposed on the model states during the updating. Our proposed model and the dual-estimation framework were verified with the NASA battery datasets. The experimental results demonstrate that our proposed method is capable of modeling capacity-regeneration phenomena and provides a good RUL-prediction performance for lithium-ion batteries.
Self-Medication Practices among Adult Population in Bangladesh: A Cross-Sectional Study
Authors: Roy Nitai , Islam Md. Nazrul , Shahjalal Md. , Siddiky Aysha , Imran Sultan Mahmud , Aktarujjaman Md. , Hossain Md. Mobarak , Rogers Bryan T. , Biswas Kamal Krishna , Hossain Ekhtear
Published: April 2024

Background: The practice of self-medication (SM) is common worldwide and is an important component of medical self-care. However, improper practice can be dangerous. This study aimed to estimate the prevalence of SM and the factors associated with it among Bangladeshi adults. Methods: A cross-sectional survey was conducted between April and June 2021 among Bangladeshi adults (aged > 19 years) using convenient sampling. A total of 1320 subjects were collected through face-to-face interviews using a standardized questionnaire. Multivariable logistic regression analysis was used to identify factors associated with the practice of SM. Results: Overall, 41% of adults in our survey reported SMP. The most common illnesses that prompted SM were common cold/flu (66.4%), gastric problems (65%), and headache (64.4%). The most frequent reasons for SM were to get better-perceived quality of care (30.6%), perceiving SM without side effects (23.3%), and saving time with effectiveness (14.56%). Potential risk factors included 10 years (AOR = 1.91; 95% CI: 1.04–3.50) and >12 years of schooling (AOR = 5.03; 95% CI: 2.27–11.15), being a businessman (AOR = 4.64; 95% CI: 1.74–12.37), having ≤6 family members (AOR = 2.13; 95% CI: 1.40–3.24), being a member of a social group (AOR = 1.53; 95% CI: 1.10–2.12), a health status check after every six months (AOR = 1.52; 95% CI: 1.08–2.13), and current ill-health (AOR = 1.41; 95% CI: 1.06–1.87). Protective factors identified included ≤30 years of age (AOR = 0.40; 95% CI: 0.17–0.93), and practice of modern (AOR = 0.39; 95% CI: 0.22–0.69) and herbal (AOR = 0.45; 95% CI: 0.21–0.97) treatment modality. Conclusion: More than one-third of the study participants reported practicing SM. Increasing the community’s awareness of the adverse outcomes of SM and not just the average experience might sway individuals away from SM, and implementing strict jurisdiction could be a way to minimize inappropriate SM.
Essential Oils: Chemistry and Food Applications
Authors: Rodilla Jesus M. , Rosado Tiago , Gallardo Eugenia
Published: April 2024

In recent years, the crucial role played by essential oils in various areas such as health, cosmetics, crop protection, and food industries has been increasingly recognized [...]
Food Hydrocolloids: Structure, Properties, and Applications
Authors: Gao Yanlei , Liu Ru , Liang Hongshan
Published: April 2024

Hydrocolloids are extensively used in the food industry for various functions, including gelling, thickening, stabilizing foams, emulsions, and dispersions, as well as facilitating the controlled release of flavor [...]
Emerging Technologies for Improving Properties, Shelf Life, and Analysis of Dairy Products
Authors: Moatsou Golfo
Published: April 2024

Processing results in several kinds of dairy products with variable properties and shelf lives that preserve and often enhance the unique nutritional and biological value of milk [...]
Evaluation of a Standard Dietary Regimen Combined with Heat-Inactivated Lactobacillus gasseri HM1, Lactoferrin-Producing HM1, and Their Sonication-Inactivated Variants in the Management of Metabolic Disorders in an Obesity Mouse Model
Authors: Shiu Wei-Chen , Liu Zhen-Shu , Chen Bo-Yuan , Ku Yu-We , Chen Po-Wen
Published: April 2024

This study investigated the impact of incorporating various inactivated probiotic formulations, with or without recombinant lactoferrin (LF) expression, into a standard chow diet on metabolic-related disorders in obese mice. After inducing obesity through a 13-week high-fat diet followed by a standard chow diet, mice received daily oral administrations of different probiotics for 6 weeks using the oral gavage approach. These probiotic formulations consisted of a placebo (MRS), heat-inactivated Lactobacillus gasseri HM1 (HK-HM1), heat-killed LF-expression HM1 (HK-HM1/LF), sonication-killed HM1 (SK-HM1), and sonication-killed LF-expression HM1 (SK-HM1/LF). The study successfully induced obesity, resulting in worsened glucose tolerance and insulin sensitivity. Interestingly, the regular diet alone improved glucose tolerance, and the addition of inactivated probiotics further enhanced this effect, with SK-HM1/LF demonstrating the most noticeable improvement. However, while regular dietary intervention alone improved insulin sensitivity, probiotic supplementation did not provide additional benefits in this aspect. Inflammation in perirenal and epididymal fat tissues was partially alleviated by the regular diet and further improved by probiotics, particularly by SK-HM1, which showed the most significant reduction. Additionally, HK-HM1 and HK-HM1/LF supplements could contribute to the improvement of serum total triglycerides or total cholesterol, respectively. Overall, incorporating inactivated probiotics into a regular diet may enhance metabolic indices, and recombinant LF may offer potential benefits for improving glucose tolerance.
Distinct Changes in Metabolic Profile and Sensory Quality with Different Varieties of Chrysanthemum (Juhua) Tea Measured by LC-MS-Based Untargeted Metabolomics and Electronic Tongue
Authors: Tian Xing , Wang Haodong , Chen Liang , Yuan Hanwen , Peng Caiyun , Wang Wei
Published: April 2024

Chrysanthemum tea, a typical health tea with the same origin as medicine and food, is famous for its unique health benefits and flavor. The taste and sensory quality of chrysanthemum (Juhua) tea are mainly determined by secondary metabolites. Therefore, the present research adopted untargeted metabolomics combined with an electronic tongue system to analyze the correlation between the metabolite profiles and taste characteristics of different varieties of chrysanthemum tea. The results of sensory evaluation showed that there were significant differences in the sensory qualities of five different varieties of chrysanthemum tea, especially bitterness and astringency. The results of principal component analysis (PCA) indicated that there were significant metabolic differences among the five chrysanthemum teas. A total of 1775 metabolites were identified by using untargeted metabolomics based on UPLC-Q-TOF/MS analysis. According to the variable importance in projection (VIP) values of the orthogonal projections to latent structures discriminant analysis (OPLS-DA), 143 VIP metabolites were found to be responsible for metabolic changes between Huangju and Jinsi Huangju tea; among them, 13 metabolites were identified as the key metabolites of the differences in sensory quality between them. Kaempferol, luteolin, genistein, and some quinic acid derivatives were correlated with the “astringency” attributes. In contrast, l-(-)-3 phenyllactic acid and L-malic acid were found to be responsible for the “bitterness” and “umami” attributes in chrysanthemum tea. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that the flavonoid and flavonol biosynthesis pathways had important effects on the sensory quality of chrysanthemum tea. These findings provide the theoretical basis for understanding the characteristic metabolites that contribute to the distinctive sensory qualities of chrysanthemum tea.
There’s Something in What We Eat: An Overview on the Extraction Techniques and Chromatographic Analysis for PFAS Identification in Agri-Food Products
Authors: Iannone Alessia , Carriera Fabiana , Passarella Sergio , Fratianni Alessandra , Avino Pasquale
Published: April 2024

Per- and polyfluorinated alkyl substances (PFASs) are a group of anthropogenic chemicals used in a range of industrial processes and consumer products. Recently, their ubiquitous presence in the environment as well as their toxicological effects in humans have gained relevant attention. Although the occurrence of PFASs is widely investigated in scientific community, the standardization of analytical method for all matrices still remains an important issue. In this review, we discussed extraction and detection methods in depth to evaluate the best procedures of PFAS identification in terms of analytical parameters (e.g., limits of detection (LODs), limits of quantification (LOQs), recoveries). Extraction approaches based on liquid–liquid extraction (LLE), alkaline digestion, and solid phase extraction (SPE), followed by liquid chromatography–mass spectrometry (LC-MS) and gas chromatography–mass spectrometry (GC-MS) analysis are the main analytical methods applied in the literature. The results showed detectable recoveries of PFOA and PFOS in meat, milk, vegetables, eggs products (90.6–101.2% and of 89.2–98.4%), and fish (96–108%). Furthermore, the low LOD and LOQ values obtained for meat (0.00592–0.01907 ng g−1; 0.050 ng g−1), milk (0.003–0.009 ng g−1; 0.010–0.027 ng g−1), fruit (0.002–0.009 ng g−1; 0.006–0.024 ng g−1), and fish (0.00369–0.017.33 ng g−1; 0.05 ng g−1) also confirmed the effectiveness of the recent quick, easy, cheap, effective, rugged, and safe method (QuEChERS) for simple, speedy, and sensitive ultra-trace PFAS analysis.
Changes in the Quality and Microflora of Yellowtail Seriola quinqueradiata Muscles during Cold Storage
Authors: Tanimoto Shota , Hirata Yuka , Ishizu Shinta , Wang Run , Furuta Ayumi , Mabuchi Ryota , Okada Genya
Published: April 2024

We evaluated the changes in the quality and microflora of yellowtail flesh cold-stored until spoilage. Based on the sensory evaluation, odor palatability was deemed unacceptable for dark muscle (DM) and the dorsal part of the ordinary muscle (OD) after >10 days and 14 of storage, respectively. Log 7 CFU/g in DM as well as OD was obtained on days 10 (Aeromonas spp.) and 14 (Enterobacteriaceae and Pseudomonas spp.) of storage, whereas log 5 (Brocothrix thermosphacta) and 6 (H2S-producing bacteria) CFU/g in them were obtained on day 14 of storage. In these bacteria, the viable bacterial counts of Pseudomonas spp. and Aeromonas spp. in DM were significantly higher than those in OD only at some storage times. Amplicon sequencing revealed that in both muscles, Pseudomonas became predominant after storage, with greater than 90% recorded after more than 10 days of storage. The relative abundances of Acinetobacter, Unclassified Gammaproteobacter, and Shewanella were relatively high in both muscles after more than 10 days of storage; however, these values were less than 5%. Ethyl butyrate in the OD and DM and 2,3-butanedione in the OD were first detected on days 14 and 10 of storage, respectively. Acetoin in the OD increased by 81-fold after 14 days of storage and was significantly increased in the DM after more than 10 days compared with the amount detected pre-storage. Volatiles, such as (E)-2-pentenal in the OD and 1-pentanol in the DM, decreased and increased linearly, respectively, throughout the 14-day storage period. Altogether, these volatile components may cause quality deterioration due to spoilage and/or lipid oxidation during cold storage of the OD and DM.
Feature Papers in Population and Evolutionary Genetics and Genomics 2023: Unraveling Population Dynamics, Diversity, and Evolutionary Paths
Authors: Kyrgiafini Maria-Anna , Mamuris Zissis
Published: April 2024

The dialogue between population genetics and evolutionary biology, which historically followed separate paths, has now developed into a complex and interdisciplinary field of study [...]
Predictive Role of Cluster Bean (Cyamopsis tetragonoloba) Derived miRNAs in Human and Cattle Health
Authors: Sahu Sarika , Rao Atmakuri Ramakrishna , Sahu Tanmaya Kumar , Pandey Jaya , Varshney Shivangi , Kumar Archna , Gaikwad Kishor
Published: April 2024

MicroRNAs (miRNAs) are small non-coding conserved molecules with lengths varying between 18-25nt. Plants miRNAs are very stable, and probably they might have been transferred across kingdoms via food intake. Such miRNAs are also called exogenous miRNAs, which regulate the gene expression in host organisms. The miRNAs present in the cluster bean, a drought tolerant legume crop having high commercial value, might have also played a regulatory role for the genes involved in nutrients synthesis or disease pathways in animals including humans due to dietary intake of plant parts of cluster beans. However, the predictive role of miRNAs of cluster beans for gene–disease association across kingdoms such as cattle and humans are not yet fully explored. Thus, the aim of the present study is to (i) find out the cluster bean miRNAs (cb-miRs) functionally similar to miRNAs of cattle and humans and predict their target genes’ involvement in the occurrence of complex diseases, and (ii) identify the role of cb-miRs that are functionally non-similar to the miRNAs of cattle and humans and predict their targeted genes’ association with complex diseases in host systems. Here, we predicted a total of 33 and 15 functionally similar cb-miRs (fs-cb-miRs) to human and cattle miRNAs, respectively. Further, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed the participation of targeted genes of fs-cb-miRs in 24 and 12 different pathways in humans and cattle, respectively. Few targeted genes in humans like LCP2, GABRA6, and MYH14 were predicted to be associated with disease pathways of Yesinia infection (hsa05135), neuroactive ligand-receptor interaction (hsa04080), and pathogenic Escherichia coli infection (hsa05130), respectively. However, targeted genes of fs-cb-miRs in humans like KLHL20, TNS1, and PAPD4 are associated with Alzheimer’s, malignant tumor of the breast, and hepatitis C virus infection disease, respectively. Similarly, in cattle, targeted genes like ATG2B and DHRS11 of fs-cb-miRs participate in the pathways of Huntington disease and steroid biosynthesis, respectively. Additionally, the targeted genes like SURF4 and EDME2 of fs-cb-miRs are associated with mastitis and bovine osteoporosis, respectively. We also found a few cb-miRs that do not have functional similarity with human and cattle miRNAs but are found to target the genes in the host organisms and as well being associated with human and cattle diseases. Interestingly, a few genes such as NRM, PTPRE and SUZ12 were observed to be associated with Rheumatoid Arthritis, Asthma and Endometrial Stromal Sarcoma diseases, respectively, in humans and genes like SCNN1B associated with renal disease in cattle.
Improved Wave Equation Analysis for Piles in Soil-Based Intermediate Geomaterials with LRFD Recommendations and Economic Impact Assessment
Authors: Kalauni Harish K. , Masud Nafis Bin , Ng Kam , Wulff Shaun S.
Published: April 2024

The Wave Equation Analysis of Pile Driving (WEAP) has been widely used to determine drivability, predict static resistance, and assure the integrity of piles in soils. Assigning static and dynamic properties of Soil-based Intermediate Geomaterials (S-IGMs) remains a challenge in WEAP, partly attributed to IGMs that act as transition geomaterials between soil and hard rock. Furthermore, reliable static analysis methods for unit resistance predictions are rarely available for driven piles in S-IGMs in the default WEAP method. To alleviate these challenges, this study presents improved WEAP methods for steel piles driven in S-IGMs, including proposed damping parameters and Load and Resistance Factor Design (LRFD) recommendations based on newly developed static analysis methods and the classification of S-IGMs. A back calculation approach is used to generate the appropriate damping parameters for S-IGMs for three distinct subsurface conditions utilizing a database of 34 steel H- and pipe piles. Newly developed WEAP and LRFD procedures are also recommended. Additional independent 22 test pile data are used to compare and evaluate the accuracy and efficiency of the proposed WEAP methods with the default WEAP method. Compared with the default WEAP, bearing graph analysis results revealed that the selected proposed WEAP method, on average, reduces the underprediction of pile resistances by 6% and improves the reliability with a 43% reduction in the coefficient of variation (COV). Calibrated resistance factors for the proposed WEAP method increase to as high as 0.75 compared to the current AASHTO recommendation of 0.50. An economic impact assessment reveals that the proposed WEAP method is more efficient than the default WEAP method as the average difference in steel weight for 32 test piles is 0.06 kg/kN, almost close to zero, reducing the construction challenges in the current engineering practice.
The 2-Minutes Walking Test Is Not Correlated with Aerobic Fitness Indices but with the 5-Times Sit-to-Stand Test Performance in Apparently Healthy Older Adults
Authors: Gil-Calvo Marina , de Paz José Antonio , Herrero-Molleda Alba , Zecchin Arthur , Gómez-Alonso María Teresa , Alonso-Cortés Beatriz , Boullosa Daniel
Published: April 2024

The 2-minutes walking test (2-MWT) is a valid and reliable test that has a high correlation with the distance walked in the 6-minutes walking test (6-MWT). However, to date, no study has determined the relationship between 2-MWT performance and the aerobic fitness indices obtained during a maximal incremental test to confirm if this test is a valid surrogate of aerobic fitness in apparently healthy older adults. The main objective of this work was to identify the factors associated to the performance in the 2-MWT, including aerobic fitness, functional and spatial-temporal gait parameters. Seventeen elderly adults performed a maximal incremental cycling test to determine maximum oxygen consumption (VO2max) and ventilatory thresholds (VT1 and VT2), two static standing balance tests with open and close eyes, a 5-times sit-to-stand test (5-TSTS), a handgrip test, and a 2-MWT on three different days over 2 weeks. No correlations were found between aerobic fitness indices and the distance covered in 2-MWT, but significant moderate correlations were found between the distance covered in 2-MWT and the time to perform the 5-TSTS (rho = −0.49) and with stride length (rho = 0.52) during the test. In conclusion, the 2-MWT does not seem a good test to assess aerobic capacity while it showed to be associated to the 5-TSTS performance of the elderly.
Associations of Anxiety, Insomnia, and Physical Activity during the COVID-19 Pandemic
Authors: Tobin Selene Y. , Halliday Tanya M. , Shoaf Kimberley , Burns Ryan D. , Baron Kelly G.
Published: April 2024

Purpose: Anxiety, insomnia, and physical activity (PA) are interrelated, but the bi-directional relationships between these three variables are not well understood. Less is known of these relationships in settings of disrupted daily activities and acute stress. This study aimed to characterize and examine relationships between insomnia, anxiety, and PA throughout the first year of the COVID-19 pandemic, when many lifestyle behaviors were disrupted. Methods: Participants comprised a convenience sample of 204 adults (55.4% female; 43.85 ± 15.85 years old) who completed the Generalized Anxiety Disorder Questionnaire (GAD-7), Insomnia Severity Index (ISI), and the International Physical Activity Questionnaire (IPAQ) at three time points through the first year of the COVID-19 pandemic. A cross-lagged panel model was used to evaluate these variables’ concurrent, autoregressive, and cross-lagged relationships across time. Follow-up dynamic panel modeling using maximum likelihood and structural equation modeling was employed. Results: Approximately 64% of participants reported their work/occupation as affected by the pandemic. At baseline, associations between anxiety and insomnia were observed (β-coefficient: 15.87; p < 0.001). Insomnia was a positive future predictor of anxiety (ISI time point 2: 7.9 ± 5.6 points; GAD-7 at time point 3: 4.1 ± 4.2 points; β-coefficient: 0.16; p < 0.01). No associations were observed between PA and anxiety or insomnia (all p > 0.05). Conclusions: Insomnia and anxiety were interrelated, and effects were cross-lagged. These data can inform future work focused on improving anxiety in settings of acute stress and disruptions to daily life, such as changes in occupational structure and stability. Specifically, targeting sleep parameters may be of interest to elicit downstream positive health behaviors.
Is There a Place for Lewy Bodies before and beyond Alpha-Synuclein Accumulation? Provocative Issues in Need of Solid Explanations
Authors: Lenzi Paola , Lazzeri Gloria , Ferrucci Michela , Scotto Marco , Frati Alessandro , Puglisi-Allegra Stefano , Busceti Carla Letizia , Fornai Francesco
Published: April 2024

In the last two decades, alpha-synuclein (alpha-syn) assumed a prominent role as a major component and seeding structure of Lewy bodies (LBs). This concept is driving ongoing research on the pathophysiology of Parkinson’s disease (PD). In line with this, alpha-syn is considered to be the guilty protein in the disease process, and it may be targeted through precision medicine to modify disease progression. Therefore, designing specific tools to block the aggregation and spreading of alpha-syn represents a major effort in the development of disease-modifying therapies in PD. The present article analyzes concrete evidence about the significance of alpha-syn within LBs. In this effort, some dogmas are challenged. This concerns the question of whether alpha-syn is more abundant compared with other proteins within LBs. Again, the occurrence of alpha-syn compared with non-protein constituents is scrutinized. Finally, the prominent role of alpha-syn in seeding LBs as the guilty structure causing PD is questioned. These revisited concepts may be helpful in the process of validating which proteins, organelles, and pathways are likely to be involved in the damage to meso-striatal dopamine neurons and other brain regions involved in PD.
Predicting Response to Immunotargeted Therapy in Endometrial Cancer via Tumor Immune Microenvironment: A Multicenter, Observational Study
Authors: Maltseva Anastasia , Kalinchuk Anna , Chernorubashkina Nataliya , Sisakyan Virab , Lots Igor , Gofman Alina , Anzhiganova Yulia , Martynova Elizaveta , Zukov Ruslan , Aleksandrova Elena , Kolomiets Larisa , Tashireva Liubov
Published: April 2024

Only one-third of patients with advanced MSS/pMMR endometrial cancer exhibit a lasting response to the combination treatment of Pembrolizumab and Lenvatinib. The combined administration of these two drugs is based on Lenvatinib’s ability to modulate the tumor microenvironment, enabling Pembrolizumab to exert its effect. These findings underscore the importance of exploring tumor microenvironment parameters to identify markers that can accurately select candidates for this type of therapy. An open non-randomized observational association study was conducted at six clinical centers, involving a total of 28 patients with advanced MSS/pMMR endometrial cancer who received Pembrolizumab and Lenvatinib therapy. Using TSA-associated multiplex immunofluorescence, we analyzed the proportion of CD8+ T lymphocytes, CD20+ B lymphocytes, FoxP3+ T regulatory lymphocytes, and CD163+ macrophages in tumor samples prior to immunotargeted therapy. The percentage of CD20+ B lymphocytes and the CD8-to-CD20 lymphocytes ratio was significantly higher in patients who responded to treatment compared to non-responders (responders vs. non-responders: 0.24 (0.1–1.24)% vs. 0.08 (0.00–0.15)%, p = 0.0114; 1.44 (0.58–2.70) arb. unit vs. 19.00 (3.80–34.78) arb. unit, p = 0.0031). The sensitivity and specificity of these biomarkers were 85.71% and 70.59%, and 85.71% and 85.71%, respectively. The proportion of CD20+ B lymphocytes and the CD8-to-CD20 lymphocytes ratio in the stroma of endometrial cancer serves as both a prognostic marker of response to immunotargeted therapy and a prognostic factor for progression-free survival in patients.
Odorant Receptors Expressing and Antennal Lobes Architecture Are Linked to Caste Dimorphism in Asian Honeybee, Apis cerana (Hymenoptera: Apidae)
Authors: Ke Haoqin , Chen Yu , Zhang Baoyi , Duan Shiwen , Ma Xiaomei , Ren Bingzhong , Wang Yinliang
Published: April 2024

Insects heavily rely on the olfactory system for food, mating, and predator evasion. However, the caste-related olfactory differences in Apis cerana, a eusocial insect, remain unclear. To explore the peripheral and primary center of the olfactory system link to the caste dimorphism in A. cerana, transcriptome and immunohistochemistry studies on the odorant receptors (ORs) and architecture of antennal lobes (ALs) were performed on different castes. Through transcriptomesis, we found more olfactory receptor genes in queens and workers than in drones, which were further validated by RT-qPCR, indicating caste dimorphism. Meanwhile, ALs structure, including volume, surface area, and the number of glomeruli, demonstrated a close association with caste dimorphism. Particularly, drones had more macroglomeruli possibly for pheromone recognition. Interestingly, we found that the number of ORs and glomeruli ratio was nearly 1:1. Also, the ORs expression distribution pattern was very similar to the distribution of glomeruli volume. Our results suggest the existence of concurrent plasticity in both the peripheral olfactory system and ALs among different castes of A. cerana, highlighting the role of the olfactory system in labor division in insects.
Embeddings of Graphs: Tessellate and Decussate Structures
Authors: O’Keeffe Michael , Treacy Michael M. J.
Published: April 2024

We address the problem of finding a unique graph embedding that best describes a graph’s “topology” i.e., a canonical embedding (spatial graph). This question is of particular interest in the chemistry of materials. Graphs that admit a tiling in 3-dimensional Euclidean space are termed tessellate, those that do not decussate. We give examples of decussate and tessellate graphs that are finite and 3-periodic. We conjecture that a graph has at most one tessellate embedding. We give reasons for considering this the default “topology” of periodic graphs.
On the Structural and Vibrational Properties of Solid Endohedral Metallofullerene Li@C60
Authors: Vrankić Martina , Nakagawa Takeshi , Menelaou Melita , Takabayashi Yasuhiro , Yoshikane Naoya , Matsui Keisuke , Kokubo Ken , Kato Kenichi , Kawaguchi-Imada Saori , Kadobayashi Hirokazu , Arvanitidis John , Kubota Yoshiki , Prassides Kosmas
Published: April 2024

The endohedral lithium fulleride, Li+@C60•−, is a potential precursor for new families of molecular superconducting and electronic materials beyond those accessible to date from C60 itself. Solid Li@C60 comprises (Li@C60)2 dimers, isostructural and isoelectronic with the (C59N)2 units found in solid azafullerene. Here, we investigate the structural and vibrational properties of Li@C60 samples synthesized by electrolytic reduction routes. The resulting materials are of high quality, with crystallinity far superior to that of their antecedents isolated by chemical reduction. They permit facile, unambiguous identification of both the reduced state of the fulleride units and the interball C-C bonds responsible for dimerization. However, severe orientational disorder conceals any crystal symmetry lowering due to the presence of dimers. Diffraction reveals the adoption of a hexagonal crystal structure (space group P63/mmc) at both low temperatures and high pressures, typically associated with close-packing of spherical monomer units. Such a situation is reminiscent of the structural behavior of the high-pressure Phase I of solid dihydrogen, H2.
Ductus Arteriosus in Fetal and Perinatal Life
Authors: Pugnaloni Flaminia , Doni Daniela , Lucente Mariella , Fiocchi Stefano , Capolupo Irma
Published: April 2024

The ductus arteriosus represents an essential vascular structure connecting the pulmonary artery and the aorta. Over the past decades, there has been substantial advancement in our understanding of both the fundamental and clinical aspects of the ductus arteriosus. In particular, the clarification of the regulatory mechanisms governing ductal patency in critical stages such as the fetal and the perinatal period has enabled optimal management of both physiological and pathological conditions in which the ductus arteriosus plays a crucial role. Furthermore, a more in-depth understanding of the regulatory mechanisms controlling this fundamental structure has facilitated the development of advanced therapeutic strategies and personalized interventions. In the present review, we provide a comprehensive overview of the ductus arteriosus during fetal and perinatal life, encompassing its physiological functions, pathological conditions, and clinical implications. Through this examination, we aim to contribute to a broader understanding of the ductus arteriosus’ role in these critical developmental stages and its significance in clinical practice.
Elevated Leukocyte Glucose Index Is Associated with Long-Term Arteriovenous Fistula Failure in Dialysis Patients
Authors: Mureșan Adrian Vasile , Florea Elena , Arbănași Emil-Marian , Bartus Réka , Arbănași Eliza-Mihaela , Ion Alexandru Petru , Cordoș Bogdan Andrei , Halatiu Vasile Bogdan , Niculescu Raluca , Stoian Adina , Ciucanu Claudiu Constantin , Russu Eliza
Published: April 2024

(1) Background: Arteriovenous fistula (AVF) is the preferred type of vascular access for dialysis in patients with end-stage kidney disease (ESKD). However, the primary patency of AVF at one year is under 70% due to several risk factors and comorbidities. Leukocyte glucose index (LGI), a new biomarker based on blood leukocytes and glucose values, has been found to be associated with poor outcomes in cardiovascular disease. The aim of this study is to analyze the impact of LGI on the long-term primary patency of AVF following dialysis initiation. (2) Methods: We conducted a retrospective observational study in which we initially enrolled 158 patients with ESKD admitted to the Vascular Surgery Department of the Emergency County Hospital of Targu Mures, Romania, to surgically create an AVF for dialysis between January 2020 and July 2023. The primary endpoint was AVF failure, defined as the impossibility of performing a chronic dialysis session due to severe restenosis or AVF thrombosis. After follow-up, we categorized patients into two groups based on their AVF status: “functional AVF” for those with a permeable AVF and “AVF failure” for those with vascular access dysfunction. (3) Results: Patients with AVF failure had a higher prevalence of atrial fibrillation (p = 0.013) and diabetes (p = 0.028), as well as a higher LGI value (1.12 vs. 0.79, p < 0.001). At ROC analysis, LGI had the strongest association with the outcome, with an AUC of 0.729, and an optimal cut-off value of 0.95 (72.4% sensitivity and 68% specificity). In Kaplan–Meier survival analyses, patients in the highest tertile (T3) of LGI had a significantly higher incidence of AVF failure compared to those in tertile 1 (p = 0.019). Moreover, we found that patients with higher baseline LGI values had a significantly higher risk of AVF failure during follow-up (HR: 1.48, p = 0.003). The association is independent of age and sex (HR: 1.65, p = 0.001), cardiovascular risk factors (HR: 1.63, p = 0.012), and pre-operative vascular mapping determinations (HR: 3.49, p = 0.037). (4) Conclusions: In conclusion, high preoperative values of LGI are positively associated with long-term AVF failure. The prognostic role of the biomarker was independent of age, sex, cardiovascular risk factors, and pre-operative vascular mapping determinations.
A Twenty-Year Follow-Up of Adults with Ebstein Anomaly with Special Focus on Supraventricular Arrhythmias, Supraventricular Arrhythmias and Effectiveness of Catheter Ablation in 20-Year Follow-Up of Adults with Ebstein Anomaly
Authors: Nartowicz Sonia Alicja , Ciepłucha Aleksandra , Waśniewski Michał , Miechowicz Izabela , Rajewska-Tabor Justyna , Bartczak-Rutkowska Agnieszka , Lesiak Maciej , Pyda Małgorzata , Trojnarska Olga
Published: April 2024

Background: Ebstein anomaly (EA) is a rare congenital heart disease characterized by the apical displacement of the tricuspid leaflets, creating an enlarged functional right atrium. Supraventricular arrhythmias (SVA) are common, and catheter ablation remains challenging. SVA is considered a risk factor for sudden cardiac death in this population. Still, there are very few real-life data on the impact of SVA treated invasively or conservatively on a patient’s prognosis. We aimed to analyze the incidence of SVA in adults with EA, evaluate the effectiveness of catheter ablation, and analyze the impact of SVA and catheter ablation on survival in this population. Methods and results: 71 pts (median age 53 years; range 24–84 years) with EA were evaluated retrospectively from 1988 to 2020. Forty patients (56.3%) had SVA, and eighteen of them (45.0%) required at least one catheter ablation (35 procedures in total). Indications for ablation were mostly intra-atrial reentrant tachycardia (IART) and atrioventricular reentrant tachycardia (AVRT) (14 pts [77.8% and 9 pts [50.0%], respectively. IART and AVRT coexisted in nine pts. One patient suffered from persistent atrial fibrillation. Procedural effectiveness was reported in 28 (80%) cases; over a longer follow-up (mean 12.6 ± 5.4 years), only eight (44.4%) patients were completely free from SVA after the first ablation. In total, 10 patients (14%) died due to cardiovascular events. There was no difference in survival between patients with or without SVA (p = 0.9) and between ablated and non-ablated EA individuals (p = 0.89). Conclusions: Supraventricular arrhythmia is frequent in adults with Ebstein anomaly. Patients often require more than one catheter ablation but eventually become free from arrhythmias. The imaging parameters assessed by echocardiography or cardiac magnetic resonance do not seem to be associated with ablation outcomes. The impact of supraventricular arrhythmia itself or treatment with radiofrequency ablation is questionable and should be thoroughly investigated in this population.
A Case Report of Secondary Glaucoma in a Patient with HTLV-1: Discussion about the Management of Secondary Glaucoma Due to Recurrent Granulomatous Uveitis
Authors: Ashikaga Kenta , Hamanaka Teruhiko , Hosogai Mayumi , Tanaka Takao , Nakao Shintaro , Funaki Toshinari
Published: April 2024

Purpose: to investigate secondary glaucoma resulting from uveitis in a patient infected with Human T-cell Leukemia Virus Type 1 (HTLV-1) pathologically and discuss the management of glaucoma with recurrent uveitis. Clinical course: An octogenarian woman diagnosed as a carrier of HTLV-1 experienced recurrent uveitis and a sudden rise in intraocular pressure (IOP) in both eyes. Due to the uncontrolled IOP and severely damaged visual field in her left eye, a combined procedure of trabeculectomy and DGIS (glaucoma drainage implant surgery, Baerveldt 350) was performed. The presence of HTLV-1 provirus was detected in the aqueous humor. Her trabeculectomy sample was processed for light microscopic observation. Following an irregular follow-up, she presented with a sudden decrease in vision and pain in her fellow eye, four years after the glaucoma surgeries. Her right eye exhibited a significant accumulation of mutton-fat-like keratic precipitates. Results: Clinical manifestations revealed the presence of granulomatous uveitis. The combined glaucoma surgery, along with continuous topical corticosteroid medication post-surgery in her left eye, effectively suppressed the high IOP spikes and the recurrence of uveitis for 4 years. The pathological examination of the outflow pathways showed a range of damages in Schlemm’s canal (SC), including SC endothelial loss, narrowing, and occlusion, as well as loss of trabecular meshwork (TM) cells and fused TM beams. Conclusion: Combined GDIS and trabeculectomy represents a promising approach for managing such refractory cases of secondary glaucoma. Continuous topical corticosteroid medication is strongly recommended to prevent irreversible changes in SC and TM associated with granulomatous uveitis.
Bilateral Carotid Calcification Correlates with Regional Cerebral Glucose Metabolism: Insights from PET/CT Imaging of Patients with Cardiovascular Risk Factors
Authors: Teichner Eric M. , Subtirelu Robert C. , Patil Shiv , Al-Daoud Omar , Parikh Chitra , Nguyen Linh , Atary Jordan , Newberg Andrew , Høilund-Carlsen Poul Flemming , Alavi Abass
Published: April 2024

Background: Cardiovascular disease is a leading cause of illness and death globally, primarily due to atherosclerosis. This disease reduces blood flow and oxygen delivery to organs, and when it affects the carotid arteries, it can lead to cognitive impairment and dementia. In a population of 104 individuals, comprising both healthy controls and individuals at elevated risk for developing cardiovascular diseases (CVD) due to identified risk factors, we used PET imaging with 18F-fluorodeoxyglucose (FDG) to assess cerebral glucose metabolism and 18F-sodium fluoride (NaF) to detect atherosclerotic calcification. Our statistical analysis revealed significant differences in metabolic activity between healthy and at-risk individuals in specific brain regions. 18F-FDG uptake in the brain varied inversely with respect to the clinical assessment of cardiovascular risk in regions such as the cuneus (β = −0.030, SE = 0.014, p = 0.035), middle occipital gyrus (β = −0.032, SE = 0.011, p = 0.005), and posterior cingulate gyrus (β = −0.032, SE = 0.015, p = 0.044). In contrast, areas including the basis pontis (β = 0.025, SE = 0.012, p = 0.038) and the pons (β = 0.034, SE = 0.013, p = 0.008) exhibited direct correlations. Notably, carotid 18F-NaF uptake had inverse associations with 18F-FDG uptake in the cerebellum (β = −0.825, SE = 0.354, p = 0.021), medulla (β = −0.888, SE = 0.405, p = 0.029), and posterior cingulate gyrus (β = −1.253, SE = 0.567, p = 0.028), while increased carotid calcification influenced metabolic activity in the fusiform gyrus (β = 1.660, SE = 0.498, p = 0.001) and globus pallidus (β = 1.505, SE = 0.571, p = 0.009). We observed that atherosclerotic plaque accumulation, especially in the carotid arteries, has potential implications for metabolic changes in brain regions governing cognition, emotion, sensory perception, and motor activities. Our findings underscore the possible early interventions that can be used to preempt or delay cognitive deterioration linked with cardiovascular ailments.
Integrating Urban Energy Resilience in Strategic Urban Planning: Sustainable Energy and Climate Action Plans and Urban Plans in Three Case Studies in Italy
Authors: Tedeschi Giovanni
Published: April 2024

Contemporary cities are facing many challenges, from social and economic issues to the new risks related to the impacts of climate change. Focusing on energy consumptions, and the related GHG emissions, cities are considered not only the main global contributors but also the areas most exposed to risks, because of their density of population and economic activities. Implementing urban planning strategies with the purpose of increasing energy efficiency and resilience overall, is, for all these reasons, considered a top priority. This paper investigates the innovative content related to the energy-efficient and energy-resilient urban planning solutions that have started to be implemented in the cities of the Emilia-Romagna region. Two kinds of planning instruments are therefore analysed: the voluntary Sustainable Energy and Climate Action Plans (SECAPs) and the mandatory General Urban Plans (GUPs), recently approved in several cities of Emilia-Romagna. A comparative analysis of three cities in the Emilia-Romagna region, Bologna, Modena, and Ravenna is proposed, looking at the strategies of their new local city plans and SECAPs with a focus on energy management and planning. The aim is to assess whether the new structure of local city plans and the influence of SECAPs could be useful in implementing such urban-energy resiliency solutions.
Censorship and Taboo Maintenance in L1 and LX Swearing
Authors: Beers Fägersten Kristy , Stapleton Karyn , Hjort Minna
Published: April 2024

In this paper, we consider the censorship of public swear word usage as a function of, and continued maintenance of, taboo with a focus on L1 and LX swearing and its management. In research with multilingual speakers, first-language swear words are consistently perceived as more taboo, and thus more emotional/powerful than equivalent words from a second or third language. While the public use of English-language swear words may be subject to censorship in Anglophone contexts, it is not censored to the same extent in LX contexts. On the other hand, L1 swear words are censored. Such perceptions of differences in strength between one’s L1 and LX languages also seem to affect the work of language professionals: translators’ tendency to self-censor may at least in part be explained by this bias. The existence of a two-tier system of swearing and censorship serves to reinvigorate L1 swear words, while diminishing the power of English swear words. We thus examine how censorship works as a means of maintaining and/or attenuating taboo, potentially moderating the power of swearing itself in cross-linguistic and multilingual contexts.
An Ab Initio Investigation of the Hydration of Antimony(III)
Authors: Pye Cory C. , Gunasekara Champika Mahesh
Published: April 2024

The energies, structures, and vibrational frequencies of [Sb(H2O)n]3+, n = 0–9, 18 have been calculated at the Hartree–Fock and second-order Møller–Plesset levels of theory using the CEP, LANL2, and SDD effective core potentials in combination with their associated basis sets, or with the 6-31G* and 6-31+G* basis sets. The metal–oxygen distances and totally symmetric stretching frequency of the aqua ions were compared with each other and with related crystal structure measurements where available.
Fabrication of Poly(ethylene furanoate)/Silver and Titanium Dioxide Nanocomposites with Improved Thermal and Antimicrobial Properties
Authors: Stanley Johan , Xanthopoulou Eleftheria , Zemljič Lidija Fras , Klonos Panagiotis A. , Kyritsis Apostolos , Lambropoulou Dimitra A. , Bikiaris Dimitrios N.
Published: April 2024

Poly(ethylene furanoate) (PEF)-based nanocomposites were fabricated with silver (Ag) and titanium dioxide (TiO2) nanoparticles by the in-situ polymerization method. The importance of this research work is to extend the usage of PEF-based nanocomposites with improved material properties. The PEF-Ag and PEF-TiO2 nanocomposites showed a significant improvement in color concentration, as determined by the color colorimeter. Scanning electron microscopy (SEM) photographs revealed the appearance of small aggregates on the surface of nanocomposites. According to crystallinity investigations, neat PEF and nanocomposites exhibit crystalline fraction between 0–6%, whereas annealed samples showed a degree of crystallinity value above 25%. Combining the structural and molecular dynamics observations from broadband dielectric spectroscopy (BDS) measurements found strong interactions between polymer chains and nanoparticles. Contact angle results exhibited a decrease in the wetting angle of nanocomposites compared to neat PEF. Finally, antimicrobial studies have been conducted, reporting a significant rise in inhibition of over 15% for both nanocomposite films against gram-positive and gram-negative bacteria. From the overall results, the synthesized PEF-based nanocomposites with enhanced thermal and antimicrobial properties may be optimized and utilized for the secondary packaging (unintended food-contact) materials.