bluuebunny/crossref_metadata_2025 huggingface dataset:
Spigelian hernia as one of the cause of recurrent abdominal pain
Authors:   Ilyinskaya Hospital, Moscow Region, Krasnogorsk, Russian Federation, Shchapov N.F. , Kulikov D.V. ,   Ilyinskaya Hospital, Moscow Region, Krasnogorsk, Russian Federation, Vyborniy M.I. ,   Ilyinskaya Hospital, Moscow Region, Krasnogorsk, Russian Federation, Bullikh P.V. ,   Ilyinskaya Hospital, Moscow Region, Krasnogorsk, Russian Federation, Keshishyan E.S. ,   Ilyinskaya Hospital, Moscow Region, Krasnogorsk, Russian Federation,   N.I.Pirogov Russian National Research Medical University, Moscow, Russian Federation, Degtyarev A.S. ,   Ilyinskaya Hospital, Moscow Region, Krasnogorsk, Russian Federation
Published: February 2024

The spigelian hernia is a rare pathology in pediatric practice. The analysis of all available publications has been carried out since the first description of this disease in children, the features of its clinical picture, diagnostic criteria and surgical tactics have been determined. The inclusion of the spigelian hernia in the list of diagnoses that must be excluded in case of recurrent abdominal pain will allow timely detection of this disease in children, timely surgical care, reduce the number of complications and improve the quality of life. Key words: abdominal pain syndrome of unclear etiology, spigelian hernia, children, laparoscopic hernia repair, testicular spigelian dystopia syndrome, traumatic hernia, ultrasound signs of hernia
Netosis-forming ability of neutrophils in children with latent tuberculosis infection with the positive test with a tuberculosis recombinant allergen
Authors:   Omsk State Medical University, Omsk, Russian Federation, Mordyk A.V. , Zolotov A.N. ,   Omsk State Medical University, Omsk, Russian Federation, Novikov D.G. ,   Omsk State Medical University, Omsk, Russian Federation, Romanova M.A. ,   Omsk State Medical University, Omsk, Russian Federation, Kirichenko N.A. ,   Omsk State Medical University, Omsk, Russian Federation, Ptukhin A.O. ,   Omsk State Medical University, Omsk, Russian Federation
Published: February 2024

Publications on the role of neutrophils in tuberculosis are contradictory. The potentially versatile role of neutrophils in the pathogenesis of tuberculosis confirms that the participation of granulocytes in tuberculosis infection is important. Patients and methods. The prospective single-center study included 24 patients with latent tuberculosis infection at the time of the first positive test for the tuberculosis recombinant allergen in the absence of radiological changes and 29 children who made up the control group according to the results of the annual tuberculin diagnostics, with a negative reaction to the tuberculosis allergen with 2TE. In the formed groups, the netosis-forming ability of an isolated fraction of neutrophils of peripheral venous blood was determined in vitro using an original technique developed by members of the author's team Results. Neutrophils of children in the study groups demonstrated different levels of netosis-forming ability, and in the group with latent tuberculosis infection, the non-forming ability of neutrophils was higher in comparison with the control group. Neutrophils of both groups formed mainly filamentous traps. In the preparation of a group of children with latent tuberculosis infection, after stimulation of granulocytes with a probiotic, there was a decrease (p = 0.0011) in the percentage of hypoactivated neutrophils relative to the control group. Conclusions. Changes in the proinflammatory and antimicrobial properties of neutrophils can become one of the markers that allow us to assess the dynamics of localization of tuberculosis. Key words: children, latent tuberculosis infection, neutrophil extracellular traps, neutrophil non-forming ability, tuberculosis
Splenomegaly: manifestation of Gaucher's disease in a 5-year-old boy
Authors:   Research Clinical Institute of Childhood, Ministry of Health of the Moscow Region, Moscow, Russian Federation, Martynovich N.N. , Odinaeva N.D. ,   Research Clinical Institute of Childhood, Ministry of Health of the Moscow Region, Moscow, Russian Federation
Published: February 2024

The article presents a clinical case of the debut of Gaucher's disease in a 5-year-old boy. Inherited by autosomal recessive type, Gaucher disease is the most common of lysosomal accumulation diseases. The frequency of occurrence is the same among both male and female individuals. This clinical case will be of interest not only for practical healthcare doctors – pediatricians, but also for ultrasound diagnostics doctors, given the presence of pronounced splenomegaly. Key words: child, lysosomal accumulation diseases, Gaucher's disease, splenomegaly, enzyme diagnostics, enzyme replacement therapy
Vacuum therapy in the treatment of wound complications after minimally invasive thoracoplasty
Authors:   M.F.Vladimirskiy Moscow Regional Research and Clinical Institute (MONIKI), Moscow, Russian Federation, Gatsutsyn V.V. , Kuzmichev V.A. ,   M.F.Vladimirskiy Moscow Regional Research and Clinical Institute (MONIKI), Moscow, Russian Federation, Pykhteev D.A. ,   M.F.Vladimirskiy Moscow Regional Research and Clinical Institute (MONIKI), Moscow, Russian Federation, Bondarenko E.V. ,   M.F.Vladimirskiy Moscow Regional Research and Clinical Institute (MONIKI), Moscow, Russian Federation, Duryagin V.D. ,   M.F.Vladimirskiy Moscow Regional Research and Clinical Institute (MONIKI), Moscow, Russian Federation
Published: February 2024

Pectus excavatum and pectus carinatum of the most common anomaly of the anterior chest wall. The most effective method of correction is considered to be surgical minimally invasive thoracoplasty by Nuss and Abramson procedure. In rare cases, postoperative complications can be in form of postoperative sutures failure of and externalization of the metal bar’s. These complications require long-term treatment, and if therapy is unsuccessful, they can lead to removal of the bar’s and, as a consequence, loss of correction. From 2006 to 2022 in the 1st Surgical Department and the Department of Pediatric Surgery of Moscow Regional Research Scientifical and Clinical Institute named after M.F. Vladimirskiy (“MONIKI”) minimal invasive corrections of chest deformity in 1229 patients were performed. Postoperative wound complications, which were poorly regenerating and infected were noted in 3.1% of cases. The use of negative pressure bandages in combination with antibiotic therapy according to the antibiogram, in combination with non-steroidal anti-inflammatory and antihistamine drugs, led in 92% of cases to complete wound healing without removal of the metal bar’s. Key words: funnel-shaped deformity, carinatum deformity, wound complications, VAC system
Possibilities of diagnostic optimization and routing improvement for children and adolescents with urticaria using current information and analytical systems
Authors:   I.M.Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation, Denisova A.R. , Geppe N.A. ,   I.M.Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation
Published: February 2024

Chronic urticaria is a common disease with a predominantly autoimmune pathogenesis. In recent decades, there has been an increase in the incidence of this disease in both adults and children. The high burden of disease in children and adolescents is due to maturation, with associated physiological and psychological changes. Several studies have demonstrated a higher risk of developing psychological disorders such as social phobia, anxiety disorder, and attention deficit hyperactivity disorder in pediatric patients suffering from chronic urticaria. They are also more likely to develop other atopic and autoimmune diseases. Timely and accurate diagnosis of chronic urticaria will allow to choose the optimal treatment tactics and reduce the risks of comorbidities. EMIAS is a unified medical information and analytical system, which was created to improve the availability and quality of medical care provided by public health institutions in Moscow. At present, continuous updating of EMIAS is actively pursued, which will help physicians of all specialties and improve the health system. This article proposes validated and simple tools whose integration into the EMIAS system will facilitate diagnosis verification and personalized (based on populationlevel recommendations) management of children with chronic urticaria at the outpatient stage by pediatricians and allergists. Key words: chronic urticaria, EMIAS, adolescents, pediatrician, allergist
Racionalizar as compras públicas para a aquisição de alimentos conforme a Agenda 2030
Authors: Bastos Bruno Costa , Vidal Marcílio Pompeu Gina
Published: February 2024

O presente artigo tem como objetivo analisar as compras públicas para adquirir alimentos por meio da racionalização sustentável em conformidade com as diretrizes da Agenda 2030 para erradicar a fome no mundo, na busca de compreender a importância da garantia de sistemas de produção de alimentos que dizem respeito em implementar práticas agrícolas que sejam resilientes. A problemática deste artigo parte da pesquisa de se empregar às compras públicas de alimentos para salvaguardar a segurança alimentar sustentável por meio de programas sociais, que busquem incentivar o consumo consciente de alimentos para preencher os requisitos da Agenda 2030 da ONU para a sustentabilidade. A metodologia utilizada é a do raciocínio indutivo e dedutivo, adotando a forma qualitativa de pesquisa, com análise documental em leis, relatórios e em artigos e livros jurídicos. Os resultados esperados é a garantia da sustentabilidade dos sistemas alimentares para combater a insegurança alimentar até 2030.Deste modo, as compras públicas para aquisição de alimentos que logra o desenvolvimento sustentável atenderam às expectativas da Agenda 2030 da ONU para um futuro mais prospero, com o objetivo acabar com a fome no mundo.
Preoperative Bladder Scanning Can Predict Postoperative Urinary Retention Following Total Joint Arthroplasty
Authors: Magaldi Robert James , Strecker Sara Elaine , Nissen Carl W. , Witmer Dan K. , Carangelo Robert James
Published: February 2024

Background:

As total joint arthroplasty (TJA) moves to the outpatient setting, it is becoming clear that postoperative urinary retention (POUR) is a potential impediment to same-day discharge. Although risk factors for POUR have been widely studied, the lack of their clinical utility warrants investigation of specific preoperative factors that can assist in surgical planning and patient optimization. The purpose of the current study was to determine whether preoperative symptom surveys and bladder scanning are useful tools in identifying POUR risk.

Methods:

We performed a prospective analysis of patients undergoing total hip arthroplasty (THA) and total knee arthroplasty (TKA) at a high-volume orthopaedic hospital between December 1, 2020, and September 30, 2021. A total of 507 patients (324 female and 183 male) undergoing TJA completed the American Urological Association (AUA) symptom index preoperatively and then again at 14 and 64 days postoperatively. Post-void bladder scans were obtained in the immediate preoperative setting. POUR was defined as a bladder volume of >500 mL that required catheterization. Chi-square and quintile analysis were used to compare bladder scanning volumes, and Student t tests were used to compare AUA scores.

Results:

The rate of POUR was 37% (66 female and 34 male) and 23% (37 female and 19 male) in the TKA and THA groups, respectively. Increasing post-void residual volume (PVRV) measured on preoperative bladder scanning was found to be predictive of POUR. Among the TKA cohort, younger age and lower body mass index were also associated with increased catheterization, although age was not statistically significant. The AUA symptom survey was not found to correlate with POUR in either population.

Conclusions:

There was a predictable and exponential increase in the rate of catheterization as preoperative PVRV increased from 50 to 200 mL. The AUA symptom score showed no utility in predicting POUR in our study population. We propose that preoperative bladder ultrasonography become standard practice in TJA, especially among patients scheduled for same-day discharge.

Level of Evidence:

Prognostic Level II. See Instructions for Authors for a complete description of levels of evidence.
The Analysis of The Performances of The Health Systems of Countries During the COVID-19 Pandemic
Authors: ARMUTCU Ebru , YİĞİT Arzu
Published: February 2024

In the study, it is aimed to analyze the impact of the COVID-19 epidemic on the health system performance of OECD countries by using multi-criteria decision-making methods. The research population consists of 38 OECD countries. In the study, using TOPSIS, COPRAS, VIKOR and GIA methods, the countries with the best performance and the safest in the COVID-19 epidemic are ranked and compared. According to the TOPSIS and VIKOR analysis applied, Germany; according to the GIA, Japan; and according to the COPRAS analysis, New Zealand are the countries with the best performance in the health system during the COVID-19 period. At the same time, in the evaluation of COVID-19 safe countries, it is concluded that Germany according to the results of TOPSIS and GIA analysis; and South Korea according to the result of VIKOR analysis are the safest countries with the best performance. The COVID-19 outbreak is thought to be a stimulus for countries to evaluate their health systems and to take the safest countries with the best performance as a guide. As a matter of fact, considering the health plans implemented by these countries, it is recommended to improve health resources in terms of quality and quantity against possible epidemic threats.
Tıbbi Tanı ve Tedavi Cihazlarının Sağlık Harcamaları Üzerindeki Etkisi
Authors: ABİŞ Taner , ÇAPAR Haşim
Published: February 2024

Tıbbi tanı cihazlarının sağlık harcamalarını arttırmadığı buna karşın tıbbi tedavi cihazlarının ise sağlık harcamalarını arttırdığı gerçeği ile bu çalışmanın amacı, OECD düzeyinde sağlık teknolojileri olan tıbbi tanı ve tedavi cihazlarının sağlık harcamalarına etkisini incelemek ve bu etkiden elde edilen veriler ile politikacılara kanıt sağlamaktır. Bu çalışma, panel veri analizi yöntemlerinden biri olan Driscoll-Kraay tahmincisi ile tamamlanmıştır. 11 OECD ülkesinin 2009-2016 yıllarına ait verileri ile analiz yapılmıştır. Elde edilen bulgulara göre Bilgisayarlı Tomografi (CT) ve Radyoterapi (RT) cihaz sayılarındaki 1 birimlik artış kişi başı sağlık harcaması yükünü (EXP) sırasıyla 50,478 ve 296,123 birim arttırmaktadır. Yatak Sayısı (BED) ve Mamografi (MAM) teknolojisine yapılan yatırımlardaki 1 birimlik artış ise kişi başı sağlık harcaması yükünü (EXP) sırasıyla 127,158 ve 8,697 birim azaltmaktadır. Sonuç olarak, tedavide kullanılan teknolojiler, sağlık harcamalarının yükünü arttırmaktadır. Dolayısı ile sağlık teknolojisi yatırımlarının birincil korunmaya dönük yapılması, tedavi edici sağlık hizmetlerine olan talebin azalmasına neden olacaktır. Bu durum, sağlık politikacılarının tıbbi tanı ve tedavi cihazlarının hastanelerdeki düşük payının yüksek bir harcama oranı ile ilişkili olduğu gerçeğini unutmadan kanıta dayalı politikalar üretmesini gerekli kılmaktadır.
Kepemimpinan Transformasional di Pondok Pesantren
Authors: Wahrudin Bambang
Published: February 2024

Islamic educational institutions that want to progress require superior leadership. For this reason, the development of leadership models for Islamic educational institutions needs to continue to be developed. Many Islamic educational institutions are unable to develop and experience setbacks due to weak leadership. Therefore, various models of Islamic leadership need to be realized as an advantage for Islamic educational institutions. One of the leadership models that is currently developing is transformational leadership, which is one of the leadership models that has succeeded in bringing Islamic educational institutions to excellence. Transformational leadership cannot be separated from the institutional environment and superior human resources so that they are able to apply the transformational model in the management of Islamic educational institutions. Therefore, it is important for the development of Islam to know the characteristics of transformational leadership. Through the qualitative research method of this literature study, the researcher used references from various data sources and then processed them in depth to find the characteristics of transformational leadership in Islam. From this research, the results showed that the characteristics of transformational leadership in Islamic educational institutions include: 1) Idealized Influence, 2) Inspirational Motivation, 3) Intellectual Stimulation, and 4) Individualized Consideration. ). Islamic educational institutions that have a transformational leadership character are institutions that have excellence and good quality educational services as well as a superior work culture.
Analisis Manajemen Mutu Sekolah Islam (Standar Kepegawaian dalam SOP SDIT Al-Uswah Surabaya)
Authors: Nurbawani Aris
Published: February 2024

Many public elementary schools in Indonesia in the 2023 PPDB will not receive any students at all. Meanwhile, SDIT Al Uswah Surabaya is different, this school is one of the Islamic schools in Surabaya which always gets quite a lot of students every year. Quality is one of the factors that makes a school attractive. One quality is the suitability of a condition to the standards used, including the suitability of the human resource standards held by SDIT Al Uswah Surabaya. This research aims to analyze the Standard Operating Procedure (SOP) for staffing standards at SDIT Al Uswah Surabaya along with its advantages and disadvantages. This research uses a qualitative approach. This research uses secondary data. Data were analyzed using human resource management theory according to Schermerhorn. The results of this research show that the SOP for staffing standards at SDIT Al Uswah Surabaya consists of 25 SOPs. The quality workforce search function consists of one SOP, the quality workforce development function consists of five SOPs, and workforce maintenance consists of twenty SOPs, and there are two SOP that can be grouped into two human resource management functions at once. The advantage of the SDIT Al Uswah Surabaya SOP is that it is prepared simply so that it is easy to understand and implement. The weakness of the SDIT Al Uswah Surabaya SOP is that there is inequality in making SOPs, namely that the maintenance SOP has more SOPs than the SOP for workforce search and development.
A machine vision-based method for recognizing standard movements in college sports tests
Authors: li Liang
Published: February 2024

Currently, the physical health of college students is extremely concerning, as a significant number of them lack physical activity, resulting in a severe decline in physical health. To promote the physical health of students and ensure the health of all people, the state has issued a policy to include the physical health of college students in the assessment and evaluation of colleges and universities, and the physical health test of college students has become an important criterion in the assessment and evaluation of different colleges and universities. However, because many test movements must be evaluated and scored by professional coaches, the formalization and marginalization of sports tests in colleges and universities has resulted from the lack of professional sports test coaches and teachers. To increase the professionalism of sports test instructors and coaches, as well as to reduce the labor costs associated with sports tests. In this paper, we propose using machine vision to recognize the sports test actions of college students by extracting the depth information of the actions via vision sensors, combining the information of human skeletal nodes, and separating the action image information from the background by choosing an appropriate depth distance threshold. Using a support vector machine (SVM) algorithm, the segmented gesture images are recognized and their normality is assessed. The conclusion of the dynamic action recognition and evaluation process is reached. Extensive testing demonstrates that this method is able to recognize test actions in real time and provide accurate scores.
Effects of microbial inoculants on the biomass and diversity of soil microbial communities: a meta-analysis
Authors: Li Chong , Chen Xinli , Jia Zhaohui , Zhai Lu , Zhang Bo , Grüters Uwe , Ma Shilin , Qian Jing , Liu Xin , Zhang Jinchi , Müller Christoph
Published: February 2024

Microbial inoculants, transplanting microorganisms from their natural habitat to improve plant performance, hold promise for sustainable agriculture and ecological restoration but also raise a potential concern as a purposeful invasion to alter soil resident communities. Current studies have mainly focused on the impact of microbial inoculants on altering soil microbial communities in various single soil conditions. However, the comprehensive impact of microbial inoculants on soil microbial community (biomass, diversity, structure, and network) under a large scale of soil resource conditions remains unknown. Through a meta-analysis of 335 studies, we found significant and positive effects of microbial inoculants on microbial biomass. More importantly, we discovered that environmental stress weakened their positive effects, while fertilizer application and the use of native microbial inoculants enhanced them. Moreover, increased initial soil nutrients amplified the positive impact of microbial inoculants on fungal biomass, actinomycete biomass, microbial biomass carbon, and microbial biomass nitrogen. Although microbial inoculants did not significantly alter microbial diversity, they induced changes in microbial community structure and bacterial composition significantly. Lastly, we showed a reduction in the complexity of bacterial networks induced by microbial inoculants, along with increased stability. Our study highlights the overall positive impact of microbial inoculants on soil microbial biomass, emphasizing the benefits of native inoculants and the importance of considering soil nutrient levels and environmental stress.
A Survey Exploring Ophthalmologists’ Attitudes and Beliefs in Performing Immediate Sequential Bilateral Cataract Surgery (ISCBS) in Singapore
Authors: Chen David , Ng Victoria , Thiyagarajan Shwetha , Tan Clement
Published: February 2024

Objectives: To evaluate current practice patterns of Immediate Sequential Bilateral Cataract Surgery (ISBCS) by ophthalmologists in Singapore and assess their attitudes towards performing ISBCS in future cataract care.
Methods: An anonymised electronic survey, modified from a similar study conducted in the United Kingdom to fit local context, was distributed to members of the College of Ophthalmologists, Academy of Medicine, Singapore, from 20 June to 1 September 2023. An initial screening question on prior experience with ISBCS directed the rest of the survey. Questions explored ophthalmologists’ current ISBCS practice patterns and the importance of factors affecting their willingness to perform ISBCS. Results were descriptively analyzed.
Results: Results collated 2 months upon survey dissemination saw a total of 58 respondents from 235 eligible members (24.7% response rate). Of these, 16 (27.6%) were currently performing ISBCS, 37 (63.8%) had never performed, and 5 (8.6%) had stopped performing. In considering ISBCS, patient convenience (n=11, 68.8%) and reduced hospital visits (n=8, 50.0%) were the most important factors ophthalmologists nominated. The most important barriers to performing ISBCS were medico-legal issues (n=31, 83.8%) and risk of endophthalmitis (n=27, 73.0%), followed by the perceived lack of evidence for its effectiveness (n=19, 51.4%).
A Transfer Learning-Based Deep Learning Model for Automated Breast Cancer Identification in Mammograms
Authors: katiyar preeti
Published: February 2024

Breast cancer is a severe health issue that affects women all over the world, underscoring the need for reliable and effective screening techniques. The early detection, diagnosis, and treatment of breast cancer are made possible by computer-aided diagnostic (CAD) systems that rely on mammograms. This study introduces a unique deep-learning model that uses transfer learning to identify and categorize breast cancer automatically. Deep convolutional neural networks have been shown in several recent studies to diagnose breast cancer in mammograms with performance comparable to or even outperforming that of human experts. To extract attributes from the Mammographic Image Analysis Society (MIAS) dataset, the proposed model uses pre-trained convolutional neural network (CNN) architectures like ResNet50 and Visual Geometry Group networks (VGG)-16. This novel deep-learning model holds significant potential for enhancing the efficiency and accuracy of breast cancer detection and classification. A preprint has previously been published [1]
Study Protocol for a Randomized Controlled Trial of the Parent Child Assistance Program: A Case Management and Home Visiting Program for People Using Substances During Pregnancy
Authors: Maher Erin J. , Stoner Susan A. , Gerlinger Julie , Ferraro A. C. , Lepper-Pappan Heather
Published: February 2024

Background
Perinatal substance use can have significant adverse effects on maternal and child health and family stability. Few interventions are specifically designed to address this significant public health problem. The Parent-Child Assistance Program (PCAP) is a three-year case management and home-visiting intervention that seeks to help persons with at-risk substance use during pregnancy to achieve and maintain substance use disorder recovery and avoid exposing future children to substances prenatally. Although the program has consistently shown substantial pre- to post-intervention improvements in its participants, PCAP remains to be tested with a rigorous randomized controlled trial (RCT). This study protocol describes a randomized controlled trial that aims to examine the effectiveness of the intervention compared to services as usual in affecting primary outcomes related to substance use and family planning. Secondary outcomes will concern connection to recovery support services and family preservation.
Methods
Using an intent-to-treat design, the study will recruit from two metro areas in Oklahoma and enroll 200 individuals who are pregnant or up to 24-months postpartum with at-risk substance use during their current or most recent pregnancy. Participants will be randomly assigned, stratified by location, to receive either PCAP or services as usual for three years. Participants in the PCAP condition will meet with their case manager approximately biweekly over the course of the intervention period, in their local communities or in their own homes whenever possible. Case managers will assist with goal setting and provide practical assistance in support of participants’ goals. Primary and secondary outcomes will be assessed at baseline and 12, 24, and 36 months post-baseline using the Addiction Severity Index interview and a self-administered survey.
Discussion
Results from this trial will help to gauge the effectiveness of PCAP in improving parent and child well-being. Results will be reviewed by federal clearinghouses on home-visiting and foster care prevention to determine the strength of evidence of effectiveness with implications for federal financing of this program model at the state level.
Trial Registration:
This study was registered at clinicaltrials.gov (NCT05534568) on 6/8/2022.
Cadmium-induced DAG-PKC/IP3-Ca2+ double signaling pathway and cell injury in gill of freshwater crabs Sinopotamon henanense
Authors: Wang Jinxiang , Wang Qian , Liu Jianxia , Wang Lan , Luo Haibo , Yu Lijuan
Published: February 2024

Cadmium (Cd) which is an extremely toxic heavy metal and widespread in the environment has been associated with numerous organs damage and even death. To evaluate the signaling pathway and the toxicity induced by Cd2+ in aquatic organisms, freshwater crabs Sinopotamon henanense were exposed to Cd2+ (0, 14.5, 29 and 58 mg/L) for 4 d. The results showed that Cd2+ induced the increase of diacylglycerol (DAG) and inositol-1,4,5-triphosphate (IP3), the rapid change of Ca2+ concentration in the cytoplasm and activation of protein kinase C (PKC) in the gill of crab. Also, Cd2+ raised the expressions of 6 genes relating with protein detoxification and folding, including cytochrome P450 (CYP450), BAG regulator 3 (BAG3), protein disulfide isomerase (PDI) and 3 genes encoding heat shock proteins (Hsp21, Hsp27 and Hsp70). And, it was observed that Cd2+ exposure aggravated filament swelling and raised the number of dead epidermal cell in gill. Meanwhile, Cd2+ exposure degraded DNA which was accompanied by a obvious decrease in ATP level, this might be the key reasons causing cell apoptosis and necrosis. In conclusion, Cd2+ triggered DAG-PKC/IP3-Ca2+ double signaling pathway, caused DNA degradation and ATP decrease, and finally induced the cell injury in S.henanense based on histological observation. This study provided more information to know the signaling events underlying Cd2+-induced cytotoxicity.
A prototype system for the removal of 137 Cs from liquid radioactive waste using reverse osmosis membrane
Authors: Shaltout mohamed , kandil shaban , El-Tabl Abdou Saad , Aglan Hany , El-Din Ahmed M. Shahr , Mohamed Yasser T
Published: February 2024

Cesium removal from aqueous solutions of radioactive waste streams is a challenge in the field of radioactive waste management; this is due to the small atomic radii of Cs+ metal ions and their high migration ability. So, the development of a withstand system for the removal of Cs+ is crucial, in the current study, the removal of radioactive cesium from aqueous solutions using an RO-TLC membrane was studied. Two modifications were conducted; the first is to enlarge the cesium metal ion radii by interacting with mono and dia basic acids namely, stearic acid, tartaric acid, citric acid, and EDTA, and the second is the modification of the RO-membrane pore size via reaction with the same acids. The modification was confirmed using SEM, FTIR, and EDX analysis techniques. The Cs+ and K+ rejection capacities and water permeability across the membrane at 1.5 bars were evaluated. Along with using the above-mentioned acids, the Cs+ metal ion retention index (RCs) was also obtained. It was found that employing EDTA as a chelating agent in an amount of 1.5 g/L in conjunction with the variation of feed content since it provided the highest value of RCs ~98% when used. Moreover, the elution of Cs+ using; water, EDTA, Ammonia, and HCl is also investigated. The optimal value of the eluent concentration was (0.25 M) HCl. Finally, Langmuir and Freundlich isotherm models were applied for better understanding of the sorption process. Results shows that the present work results are more fitted to Langmuir isotherm model determine the dominance of the chemical sorption mechanism.
Host DNA depletion assisted metagenomic sequencing of bronchoalveolar lavage fluids for diagnosis of pulmonary tuberculosis
Authors: Yuan Jinfeng , Ma LiPing , Du Juan , Sun Hailin , Zhou Gang , Rao Guanhua , Sun Fengshuo , Chen Wangyang , Miao Hui , Tian Dan , Cheng Changhao , Wang Yan , Li Liang , Pang Yu , Li Lifeng
Published: February 2024

[Background]Metagenomic next-generation sequencing (mNGS) has greatly improved our understanding of pathogens in infectious diseases such as pulmonary tuberculosis (PTB). However, high human DNA background (> 95%) impedes the detection sensitivity of mNGS in identifying intracellular Mycobacterium tuberculosis (MTB), posing a pressing challenge for MTB diagnosis. Therefore, there is an urgent need to improve MTB diagnosis performance in PTB patients. [Methods]In this study, we optimized mNGS method for diagnosis of PTB. This led to the development of the host DNA depletion assisted mNGS (HDA-mNGS) technique, which we compared with conventional mNGS and the host DNA depletion-assisted Nanopore sequencing (HDA-Nanopore) in diagnostic performance. [Results]We collected 105 bronchoalveolar lavage fluid (BALF) samples from suspected PTB patients across three medical centers to assess the clinical performance of these methods. The results of our study showed that HDA-mNGS had the highest sensitivity(71.95%) and accuracy(74.49%) in PTB detection. This was significantly higher compared to mNGS (51.22%, 58.16%) and HDA-Nanopore (58.54%, 62.24%). Furthermore, HDA-mNGS provided an increased coverage of the MTB genome by up to 16-fold. Antibiotic resistance gene analysis indicated that HDA-mNGS could provide increased depth to the detection of AMR locus more effectively. [Conclusion]These findings indicate that HDA-mNGS can significantly improve the clinical performance of PTB diagnosis for BALF samples, offering great potential in managing antibiotic resistance in PTB patients.
Effect of non-linear thermal radiation on Prandtl-Eyring nanofluid through a stretching cylinder with entropy generation
Authors: Ullah Zakir , Zaman Gul , Ullah Ikram , Ali Ahmed Refaie
Published: February 2024

Our intention in the present work study is to investigate entropy generation on the magnetohydrodynamics (MHD) flow of Prandtl-Eyring nanofluid on a horizontal stretched cylinder. The chemical reaction and non-linear thermal radiation are also taken into consideration.The boundary layer flow equations are formulated as partial differential equations (PDEs) that are reduced and parameterized to a system of ordinarydifferential equations (ODEs) by employing mathematical transformations. Numerical solutions of resulting non-linear ODEs are carried out using theshooting technique. The results of various emerging thermophysical parameters on flow profiles and entropy generation are displayed graphically, whilephysical quantities of engineering interest are calculated through tables with physical descriptions. The significant results of this study disclose that the entropy generation number rises with rising diffusion parameter, Prandtl-Eyring fluid parameter, and Brinkman number values. The outcomes alsoreveal that a rise in the temperature ratio parameter, Eckert number, and radiation parameter boosts the temperature of the Prandtl-Eyring nanofluid.The Prandtl-Eyring nanofluid concentration significantly dropped with the rising chemical reaction parameter, but the Sherwood number showed the oppositebehavior for the rising chemical reaction parameter.
Examining Antigenic Similarities: Comparative Analysis of the Light Chain Lectin of Entamoeba histolytica and p53 Protein on the Surface of Colon Adenocarcinoma Cells Biopsy
Authors: Haghighi Leila , Dalimi Abdolhossein , Pirestani Majid , Ghaffarifar Fatemeh
Published: February 2024

Background
Entamoeba histolytica ranks as the third leading cause of death among parasitic infections worldwide. Despite its significance, the impact of this protozoan on colorectal cancer incidence and its association with the p53 protein in affected patients remain insufficiently explored. Therefore, this study aims to investigate the presence of the E. histolytica lectin light chain antigen and the p53 protein in colorectal cancer biopsies, with the objective of identifying any antigenic similarity between this protozoan and the surface proteins of tumor cells.
Materials and Methods
A total of 150 colorectal cancer biopsy samples were subjected to examination for the specific E. histolytica antigen and p53 protein through the application of the immunohistochemical technique. To augment diagnostic precision, the western blotting method was employed and its results were compared with those obtained from immunohistochemistry (IHC).
Results
Among the 150 biopsy samples examined, 100 cases tested positive for the p53 protein, while 19 cases exhibited positivity for the E. histolytica lectin antigen. Notably, only 14 cases demonstrated a positive presence for both the p53 protein and the E. histolytica protozoan.
Conclusion
Entamoeba histolytica, a leading cause of parasitic infections, raises questions about its potential link to colorectal cancer, specifically in connection with the p53 protein. This study aims to explore the antigenic similarity between the lectin of E. histolytica and the p53 protein on colorectal adenocarcinoma cells, shedding light on the intricate interactions between this protozoan and cancer cells. The findings presented in this investigation signal the need for further research to comprehensively understand the implications of E. histolytica in colorectal cancer diagnosis and treatment strategies.
Positional analysis of assisting muscles for handling-assisted exoskeleton
Authors: Wang Zheng , Guan Xiaorong , He Long , Zhu Meng , bai yu
Published: February 2024

In order to better design the Handling-assisted exoskeleton, it is necessary to analyze the biomechanics of humane handling movements. In this study, Anybody Modeling System(AMS) simulation was used to analyze the movement state of muscles during humane handling. Combined with surface electromyography(sEMG) experiments, specific analysis and verification were carried out to obtain the position of muscles that the human body needs to assist during handling. In this study, the simulation and experiment were carried out for the manual handling process. The treatment group and the experimental group were set up. This study found that the vastus medialis muscle, vastus lateralis muscle, latissimus dorsi muscle, trapezius muscle, deltoid muscle and triceps brachii muscle needs more power in the process of handling, and it is reasonable and effective to combine sEMG signals with simulation of the musculoskeletal model to analyze the muscle condition of humane movement.
PEAK Mood, Mind and Marks: Development of an Intervention to Support University Students’ Mental and Cognitive Health through Physical Exercise
Authors: Brown Catherine E. B. , Richardson Karyn , Halil-Pizzirani Bengianni , Hughes Sam , Atkins Lou , Perowne Rachel , Pitt Joseph , Yücel Murat , Segrave Rebecca A.
Published: February 2024

Background Concerns about mental and cognitive health are common among university students. Engaging in regular physical exercise has been shown to enhance both mental health and cognitive performance, yet most students are not participating in the level of exercise required to obtain these benefits. The Behaviour Change Wheel (BCW) provides a framework for developing behavioural interventions that are informed by theory, evidence, and stakeholder perspectives. The current study applied the BCW to develop the PEAK Mood, Mind and Marks program (i.e., PEAK), an intervention designed to increase university students’ exercise for the benefit of their mental and cognitive health. Methods PEAK was developed across three stages of the BCW: 1) understand the target behaviour, 2) identify intervention options, and 3) identify intervention content and delivery mode. Development was informed by triangulated data from a systematic literature review, co-design consultations with key stakeholders, and knowledge of relevant experts. Consultations with stakeholders involved focus groups with 25 university students and individual interviews with 10 university leaders and staff to identify barriers and facilitators to students’ exercise engagement and the adoption and implementation of PEAK by universities. Template analysis was used to code transcripts to the capability, opportunity, and motivation (COM-B) model of behaviour. The BCW was applied to identify the most appropriate intervention types and behaviour change techniques (BCTs). Results Thirty-one barriers and facilitators were identified and mapped to seven intervention types (Education; Modelling; Persuasion; Environmental Restructuring; Incentivisation; Training; and Enablement) and 26 BCTs, which were delivered across digital and in-person channels. The final intervention consisted of multiple components targeting students’ capability (e.g., increasing knowledge about the mental and cognitive health benefits of exercise), opportunity (e.g., providing a flexible range of accessible exercise options and social support), and motivation (e.g., increasing the perceived importance of exercise) to exercise. Conclusions University students and staff describe a need and appetite for more empowering, scalable solutions to support students’ mental and cognitive health. Exercise-based approaches that are informed by theory, evidence, and stakeholder perspectives, such as PEAK, have the potential to address this need. Current findings will inform a pilot of PEAK to evaluate its efficacy and implementation.
Selection of odors in multimedia based on correspondence with the odor categories of objects in scenes
Authors: Kim Kwangsu , Bae Jisub , Lee JeeWon , Moon Sun Ae , Lee Sang-ho , Kang Won-seok , Moon Cheil
Published: February 2024

Unlike many human senses, multimedia primarily engages the visual and auditory faculties. To broaden the sensory experience influenced by multimedia, it has incorporated olfactory stimulation to enhance the sense of reality. Odors are typically matched with objects in scenes. However, it is impractical to include all odors corresponding to every object in a scene for viewers. Alternatively, researchers propose presenting a singular odor from a category, representative of others within that category. Yet, it remains uncertain whether viewers' reactions to videos featuring multiple odors (e.g., rose, lavender, and lily) from a specific category (e.g., flower) are comparable. Therefore, we investigated whether odors within a given category could exhibit similarity in congruency based on the electroencephalogram (EEG) data's five frequency bands (delta, theta, alpha, beta, and gamma) collected as viewers watched videos. Through questionnaires and EEG experiments, we sought to comprehend the impact of similar odors within categories. It was observed that odors within a specific category were more congruent with videos than those from different odor categories. The delta and theta bands predominantly clustered in EEG data when odors from similar categories were presented to viewers. The theta band, linked to neural signals of odors during olfactory processing, played a significant role. However, despite their association with human emotional responses, the alpha, beta, and gamma bands did not exhibit clustering based on category. Our findings demonstrate the viability of selecting odors based on their categories in multimedia.
Globosus polychromus gen. et sp. nov. for a freshwater hyphomycete in Muyocopronaceae (Muyocopronales)
Authors: Xu Rong-Ju , Calabon Mark S , Mapook Ausana , Boonmee Saranyaphat , Zhou De-Qun , Hyde Kevin D , Zhao Qi
Published: February 2024

A new genus Globosus with a new species G. polychromus is introduced from a freshwater habitat in Thailand, based on morphological characters with evidence from phylogenetic analysis of concatenated internal transcribed spacer region ITS4-5.8S -ITS5 (ITS), nuclear large subunit rDNA (28S) and nuclear small subunit rDNA (18S) sequence data. Phylogenetically, Globosus clustered with Quadrisporellaand Setoapiospora in Muyocopronaceae with 100% ML/ 1.00 BYPP support. However, there are differences in morphology. Globosus is characterized by its hyphomycetous structure and having pink or yellow colonies in culture on the natural substrate and PDA, its conidiogenous cells exhibit distinctive globular intumescence which differs from existing genera in Muyocopronaceae. Descriptions and illustrations of G. polychromus are provided with a key to asexual genera in Muyocopronaceae.
Identification and Validation of Mitochondrial Oxidative Stress- Related Prognostic Signature with Clinical Characteristics and Immune Filtration in Liver Hepatocellular Carcinoma
Authors: Wang Zhihui , Zhou Hao , Zhang Lie , Liu Xin , Wang Hui
Published: February 2024

Mitochondrial oxidative stress plays a critical role in cancer development and progression. However, there is limited research on the relationship between mitochondrial oxidative stress and liver hepatocellular carcinoma (LIHC). Mitochondrial oxidative stress-related genes were collected from Genecards Portal. Prognosis-linked genes (PLGs) were identified by univariate Cox regression analysis. A risk model was constructed based on the PLGs using least absolute shrinkage and selection operator (LASSO) analysis. Receiver operating characteristic (ROC) curves were used to determine the predictive ability of the model. The expression levels of the prognostic genes were verified in the cell lines. We constructed a novel risk model based on 9 prognostic genes (CYP2C19, CASQ2, LPL, TXNRD1, CACNA1S, SLC6A3, OXTR, BIRC5, and MMP1). Survival analysis showed that patients with a low-risk score had a much better overall survival (OS). Prognostic risk score was found to be an independent predictor of prognosis. Patients in the high-risk group had a less favorable tumor microenvironment characterized by a lower degree of immune cell infiltration. In contrast, the low-risk group demonstrated a higher degree of immune cell infiltration, which could potentially contribute to a more effective antitumor immune response. Our investigation reveals the oncogenic role of mitochondrial oxidative stress in LIHC. For the first time, we established a risk prediction model for mitochondrial oxidative stress in patients with LIHC.
Risk of Death from Venous Thromboembolism in Severe and Critical Pneumonia Patients: A Retrospective Observational Study
Authors: Zhang Yang , Qu Yali , Li Tan , Liao Chuanjun , Song Shenghan , Sun Mingsheng , Zhang Wangde , Li Shuo
Published: February 2024

Background: Venous thromboembolism (VTE) poses a significant risk to patients with severe and critical pneumonia, contributing to increased mortality rates. Early detection of VTE in these patients is crucial for implementing timely interventions and improving outcomes.
Method: We conducted a single-centered, retrospective, observational study involving 32 adult patients with severe and critical pneumonia admitted to the intensive care unit of Beijing Chaoyang Hospital, China, between January 1, 2020, and February 20, 2023. All patients underwent bilateral lower extremity venous ultrasound and D-dimer testing upon admission. Logistic regression analysis was performed to assess the relationship between patient mortality and VTE, considering gender, age, time to ultrasound examination, site of thrombus, and D-dimer levels.
Results: Among the 32 patients, 21 (65.6%) developed acute venous thrombosis in both lower extremities, with varying degrees of involvement. Notably, 11 (52.3%) of these patients died during the study period. The logistic regression analysis identified standard deviation for D-dimer levels as a significant predictor of patient mortality (P = 0.05), indicating a strong association between elevated D-dimer levels and increased risk of death.
Conclusions: Our findings underscore the importance of early detection and management of VTE in severe and critical pneumonia patients. Elevated D-dimer levels, indicative of a hypercoagulable state, were significantly associated with mortality risk in this population. Prompt initiation of anticoagulant therapy upon VTE diagnosis may help mitigate this risk and improve patient outcomes. These results emphasize the need for vigilant monitoring and proactive measures to prevent VTE-related complications in critically ill pneumonia patients.
Trial Registration: Retrospectively registered