bluuebunny/crossref_metadata_2025 huggingface dataset:
Software-Automatized Individual Lactation Model Fitting, Peak and Persistence and Bayesian Criteria Comparison for Milk Yield Genetic Studies in Murciano-Granadina Goats
Authors: Pizarro Inostroza María Gabriela , Navas González Francisco Javier , Landi Vincenzo , León Jurado José Manuel , Delgado Bermejo Juan Vicente , Fernández Álvarez Javier , Martínez Martínez María del Amparo
Published: September 2020

SPSS model syntax was defined and used to evaluate the individual performance of 49 linear and non-linear models to fit the lactation curve of 159 Murciano-Granadina goats selected for genotyping analyses. Lactation curve shape, peak and persistence were evaluated for each model using 3107 milk yield controls with an average of 3.78 ± 2.05 lactations per goat. Best fit (Adjusted R2) values (0.47) were reached by the five-parameter logarithmic model of Ali and Schaeffer. Three main possibilities were detected: non-fitting (did not converge), standard (Adjusted R2 over 75%) and atypical curves (Adjusted R2 below 75%). All the goats fitted for 38 models. The ability to fit different possible functional forms for each goat, which progressively increased with the number of parameters comprised in each model, translated into a higher sensitivity to explaining curve shape individual variability. However, for models for which all goats fitted, only moderate increases in explanatory and predictive potential (AIC, AICc or BIC) were found. The Ali and Schaeffer model reported the best fitting results to study the genetic variability behind goat milk yield and perhaps enhance the evaluation of curve parameters as trustable future selection criteria to face the future challenges offered by the goat dairy industry.
Oral Conditions and Oral Health-Related Quality of Life of People with Ehlers-Danlos Syndromes (EDS): A Questionnaire-Based Cross-Sectional Study
Authors: Hanisch Marcel , Blanck-Lubarsch Moritz , Bohner Lauren , Suwelack Dominik , Kleinheinz Johannes , Köppe Jeanette
Published: September 2020

Background and objective: To date, there have only been a few studies on oral health-related quality of life (OHRQoL) of people with Ehlers–Danlos syndromes (EDS) and oral conditions. The aim of this study was, therefore, to analyze the OHRQoL of people with EDS from their own point of view as well as obtain information about their age at the time of the diagnosis, the period of time until diagnosis, and the presence of oral conditions (if any) and their association with oral health quality. Methods: The study was designed as an anonymous questionnaire-based cross-sectional study. We conducted a descriptive analysis of the Oral Health Impact Profile-14 (OHIP-14) scores, age of the participants, age at diagnosis, and the time-period between the first signs of the disease and the diagnosis of EDS. To verify the differences in OHIP-14 scores between patients with and without oral conditions, a Mann–Whitney U test was performed. A multivariate quantile (median) regression analysis was performed to evaluate the effect of different general characteristics (gender, age, and the presence of oral conditions) on the OHIP 14 scores. Furthermore, using a Mann–Whitney U test, the influence of different oral conditions was verified by testing the differences between patients without any oral conditions and patients with a specific diagnosis. Results: A total of 79 evaluable questionnaires from 66 female (83.5%) and 13 male (16.5%) participants were analyzed. On average, after the first condition, it takes 18.36 years before EDS are correctly diagnosed. Oral conditions were described by 69.6% of the participants. The median (interquartile range) OHIP-14 score was eight (ten) points for patients without oral conditions and 19 (15) for patients with oral conditions. The multivariable quantile regression shows a statistical notable association between OHIP-14 score and oral conditions (p < 0.001). OHIP-14 scores for dysgnathia, periodontitis, TMD (Temporomandibular dysfunction), a high-arched palate, malocclusion, and the anomaly of tooth formation were statistical notably different between the participants with and the participants without oral conditions. Conclusions: Long diagnostic pathways seem to be a typical problem in patients with EDS. Oral conditions associated with the underlying disease occurred regularly and showed a negative correlation with OHRQoL.
Analysis of Sheet Metal Forming (Warm Stamping Process): A Study of the Variable Friction Coefficient on 6111 Aluminum Alloy
Authors: Dou Shasha , Wang Xiaoping , Xia Jason , Wilson Lisa
Published: September 2020

Aluminum alloy materials have been widely used in automobile, aerospace and other fields because of their low density, high specific strength and corrosion resistance. The process of the warm forming of aluminum alloy improves the formability of aluminum alloy sheets, reduces the deformation resistance and spring-back and improves the forming accuracy and quality of parts. For these reasons, it is frequently used. In this work, the effects of temperature, sliding speed and normal load on the friction coefficient of 6111 aluminum alloy were studied by using a CFT-I (Equipment Type) friction tester under boundary lubrication conditions. The surface morphology of the sample after the friction test was observed by optical microscopy. The results show that the surface quality of aluminum alloy is better at 200 °C, which was used as the temperature in the experiments. According to the test measurement results, the friction coefficient increases with the increase in temperature and decreases with the increase in sliding speed and normal load. Variable friction coefficient models of sliding speed and normal load were established. Using the optimal parameter combination as the simulation parameter, the established variable friction coefficient models were input into numerical simulation software, and two sets of comparative simulations were established. The thickness distribution of the sheet material obtained through the simulation was compared with the actual test measurement. Further verification was carried out through the amount of spring-back. The results show that the thickness distribution and spring-back of the sheet obtained by the variable friction coefficient model are closer to the actual measurements (the error of the spring-back angle decreased from more than 20% to less than 10%), which verifies the reliability and accuracy of the variable friction coefficient model.
Low Molecular Weight Volatile Organic Compounds Indicate Grazing by the Marine Rotifer Brachionus plicatilis on the Microalgae Microchloropsis salina
Authors: Fisher Carolyn L. , Lane Pamela D. , Russell Marion , Maddalena Randy , Lane Todd W.
Published: September 2020

Microalgae produce specific chemicals indicative of stress and/or death. The aim of this study was to perform non-destructive monitoring of algal culture systems, in the presence and absence of grazers, to identify potential biomarkers of incipient pond crashes. Here, we report ten volatile organic compounds (VOCs) that are robustly generated by the marine alga, Microchloropsis salina, in the presence and/or absence of the marine grazer, Brachionus plicatilis. We cultured M. salina with and without B. plicatilis and collected in situ volatile headspace samples using thermal desorption tubes over the course of several days. Data from four experiments were aggregated, deconvoluted, and chromatographically aligned to determine VOCs with tentative identifications made via mass spectral library matching. VOCs generated by algae in the presence of actively grazing rotifers were confirmed via pure analytical standards to be pentane, 3-pentanone, 3-methylhexane, and 2-methylfuran. Six other VOCs were less specifically associated with grazing but were still commonly observed between the four replicate experiments. Through this work, we identified four biomarkers of rotifer grazing that indicate algal stress/death. This will aid machine learning algorithms to chemically define and diagnose algal mass production cultures and save algae cultures from imminent crash to make biofuel an alternative energy possibility.
Mycoplasmas–Host Interaction: Mechanisms of Inflammation and Association with Cellular Transformation
Authors: Benedetti Francesca , Curreli Sabrina , Zella Davide
Published: September 2020

Mycoplasmas are the smallest and simplest self-replicating prokaryotes. Located everywhere in nature, they are widespread as parasites of humans, mammals, reptiles, fish, arthropods, and plants. They usually exhibiting organ and tissue specificity. Mycoplasmas belong to the class named Mollicutes (mollis = soft and cutis = skin, in Latin), and their small size and absence of a cell wall contribute to distinguish them from other bacteria. Mycoplasma species are found both outside the cells as membrane surface parasites and inside the cells, where they become intracellular residents as “silent parasites”. In humans, some Mycoplasma species are found as commensal inhabitants, while others have a significant impact on the cellular metabolism and physiology. Mollicutes lack typical bacterial PAMPs (e.g., lipoteichoic acid, flagellin, and some lipopolysaccharides) and consequently the exact molecular mechanisms of Mycoplasmas’ recognition by the cells of the immune system is the subjects of several researches for its pathogenic implications. It is well known that several strains of Mycoplasma suppress the transcriptional activity of p53, resulting in reduced apoptosis of damaged cells. In addition, some Mycoplasmas were reported to have oncogenic potential since they demonstrated not just accumulation of abnormalities but also phenotypic changes of the cells. Aim of this review is to provide an update of the current literature that implicates Mycoplasmas in triggering inflammation and altering critical cellular pathways, thus providing a better insight into potential mechanisms of cellular transformation.
Evaluation of Antiviral, Antibacterial and Antiproliferative Activities of the Endophytic Fungus Curvularia papendorfii, and Isolation of a New Polyhydroxyacid
Authors: Khiralla Afra , Spina Rosella , Varbanov Mihayl , Philippot Stéphanie , Lemiere Pascal , Slezack-Deschaumes Sophie , André Philippe , Mohamed Ietidal , Yagi Sakina Mohamed , Laurain-Mattar Dominique
Published: September 2020

An endophytic fungus isolated from Vernonia amygdalina, a medicinal plant from Sudan, was taxonomically characterized as Curvularia papendorfii. Ethyl acetate crude extract of C. papendorfii revealed an important antiviral effect against two viral pathogens, the human coronavirus HCoV 229E and a norovirus surrogate, the feline coronavirus FCV F9. For the last one, 40% of the reduction of the virus-induced cytopathogenic effect at lower multiplicity of infection (MOI) 0.0001 was observed. Selective antibacterial activity was obtained against Staphylococcus sp. (312 µg/mL), and interesting antiproliferative activity with half maximal inhibitory concentration (IC50) value of 21.5 ± 5.9 µg/mL was observed against human breast carcinoma MCF7 cell line. Therefore, C. papendorfii crude extract was further investigated and fractionated. Twenty-two metabolites were identified by gas chromatography coupled to mass spectrometry (GC–MS), and two pure compounds, mannitol and a new polyhydroxyacid, called kheiric acid, were characterized. A combination of spectroscopic methods was used to elucidate the structure of the new aliphatic carboxylic acid: kheiric acid (3,7,11,15-tetrahydroxy-18-hydroxymethyl-14,16,20,22,24-pentamethyl-hexacosa-4E,8E,12E,16,18-pentaenoic acid). Kheiric acid showed an interesting result with a minimum inhibitory concentration (MIC) value of 62.5 µg/mL against meticillin-resistant Staphylococcus aureus (MRSA). Hence, endophytes associated with medicinal plants from Sudan merit more attention, as they could be a treasure of new bioactive compounds.
Quantitative Detection of Plasmodium falciparum Using, LUNA-FL, A Fluorescent Cell Counter
Authors: Hashimoto Muneaki , Yokota Kazumichi , Kajimoto Kazuaki , Matsumoto Musashi , Tatsumi Atsuro , Yamamoto Kenichi , Hyodo Tomonori , Matsushita Kiichiro , Minakawa Noboru , Mita Toshihiro , Oka Hiroaki , Kataoka Masatoshi
Published: September 2020

The microscopic examination of Giemsa-stained thin and/or thick blood films (Giemsa microscopy) is the standard method of malaria diagnosis. However, the results of the diagnosis significantly depend on the skills of clinical technicians. Furthermore, sample preparation and analysis are laborious and time-consuming. Therefore, in this study, we investigated if a commercially available fluorescent cell counter, LUNA-FL, was useful for the detection of Plasmodium parasite and the estimation of parasitemia. Whole blood samples from uninfected persons, spiked with P. falciparum-infected erythrocytes, were analysed. Most of the leucocytes and platelets were removed from whole blood samples with SiO2-nanofiber filters set on spin columns. The filtered samples were stained with acridine orange, and automatic detection, as well as counting of erythrocytes and parasites, were performed using LUNA-FL. Whole blood, with various levels of parasites, was analysed by Giemsa microscopy or with LUNA-FL to estimate parasitemia, and a comparative analysis was performed. The coefficient determination value of the regression line was high (R2 = 0.98), indicating that accurate quantitative parasite detection could be performed using LUNA-FL. LUNA-FL has a low running cost; it is compact, fast, and easy to operate, and may therefore be useful for point-of-care testing in the endemic areas.
It Is Our Turn to Get Cannabis High: Put Cannabinoids in Food and Health Baskets
Authors: Salami Seyed Alireza , Martinelli Federico , Giovino Antonio , Bachari Ava , Arad Neda , Mantri Nitin
Published: September 2020

Cannabis is an annual plant with a long history of use as food, feed, fiber, oil, medicine, and narcotics. Despite realizing its true value, it has not yet found its true place. Cannabis has had a long history with many ups and downs, and now it is our turn to promote it. Cannabis contains approximately 600 identified and many yet unidentified potentially useful compounds. Cannabinoids, phenolic compounds, terpenoids, and alkaloids are some of the secondary metabolites present in cannabis. However, among a plethora of unique chemical compounds found in this plant, the most important ones are phytocannabinoids (PCs). Over hundreds of 21-22-carbon compounds exclusively produce in cannabis glandular hairs through either polyketide and or deoxyxylulose phosphate/methylerythritol phosphate (DOXP/MEP) pathways. Trans-Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) are those that first come to mind while talking about cannabis. Nevertheless, despite the low concentration, cannabinol (CBN), cannabigerol (CBG), cannabichromene (CBC), tetrahydrocannabivarin (THCV), cannabidivarin (CBDV), cannabinodiol (CBND), and cannabinidiol (CBDL) may have potentially some medical effects. PCs and endocannabinoids (ECs) mediate their effects mainly through CB1 and CB2 receptors. Despite all concerns regarding cannabis, nobody can ignore the use of cannabinoids as promising tonic, analgesic, antipyretic, antiemetic, anti-inflammatory, anti-epileptic, anticancer agents, which are effective for pain relief, depression, anxiety, sleep disorders, nausea and vomiting, multiple sclerosis, cardiovascular disorders, and appetite stimulation. The scientific community and public society have now increasingly accepted cannabis specifically hemp as much more than a recreational drug. There are growing demands for cannabinoids, mainly CBD, with many diverse therapeutic and nutritional properties in veterinary or human medicine. The main objective of this review article is to historically summarize findings concerning cannabinoids, mainly THC and CBD, towards putting these valuable compounds into food, feed and health baskets and current and future trends in the consumption of products derived from cannabis.
Evaluation of Antioxidant and Anti-Inflammatory Activity of Anthocyanin-Rich Water-Soluble Aronia Dry Extracts
Authors: Banach Mariusz , Wiloch Magdalena , Zawada Katarzyna , Cyplik Wojciech , Kujawski Wojciech
Published: September 2020

Aronia fruits contain many valuable components that are beneficial to human health. However, fruits are characterized by significant variations in chemical composition dependent on the growing conditions and harvesting period. Therefore, there is a need to formulate the extracts with a precisely defined content of health-promoting substances. Aronia dry extracts (ADE) were prepared from frozen pomace applying water extraction, followed by purification and spray-drying. Subsequently, the content of anthocyanins, phenolic acids, and polyphenols was determined. The high-quality chokeberry pomace enabled obtaining extracts with anthocyanin content much higher than the typical market standards. Moreover, it was found that the antioxidant capacity of aronia extracts exceeded those found in other fruit preparations. Antioxidant and free-radical scavenging properties were evaluated using a 2,2′-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay. The inhibition of lipid peroxidation and the level of inflammatory markers have been also investigated using lipopolysaccharide (LPS)-stimulated RAW 264 cells. It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions. Additionally, it was confirmed that ADE at all analyzed concentrations did not show any cytotoxic effect as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
Physical Properties and Biofunctionalities of Bioactive Root Canal Sealers In Vitro
Authors: Jo Seung Bin , Kim Hyun Kyung , Lee Hae Nim , Kim Yu-Jin , Dev Patel Kapil , Campbell Knowles Jonathan , Lee Jung-Hwan , Song Minju
Published: September 2020

Calcium silicate-based bioactive glass has received significant attention for use in various biomedical applications due to its excellent bioactivity and biocompatibility. However, the bioactivity of calcium silicate nanoparticle-incorporated bioactive dental sealer is not much explored. Herein, three commercially available bioactive root canal sealers (Endoseal MTA (EDS), Well-Root ST (WST), and Nishika Canal Sealer BG (NBG)) were compared with a resin-based control sealer (AH Plus (AHP)) in terms of physical, chemical, and biological properties. EDS and NBG showed 200 to 400 nm and 100 to 200 nm nanoparticle incorporation in the SEM image, respectively, and WST and NBG showed mineral deposition in Hank’s balanced salt solution after 28 days. The flowability and film thickness of all products met the ISO 3107 standard. Water contact angle, linear dimensional changes, and calcium and silicate ion release were significantly different among groups. All bioactive root canal sealers released calcium ions, while NBG released ~10 times more silicon ions than the other bioactive root canal sealers. Under the cytocompatible extraction range, NBG showed prominent cytocompatibility, osteogenecity, and angiogenecity compared to other sealers in vitro. These results indicate that calcium silicate nanoparticle incorporation in dental sealers could be a potential strategy for dental periapical tissue regeneration.
Characteristics of Allergen Labelling and Precautionary Allergen Labelling in Packaged Food Products Available in Latin America
Authors: Ontiveros Noé , Gallardo Jesús Aristeo-López , Arámburo-Gálvez Jesús Gilberto , Beltrán-Cárdenas Carlos Eduardo , Figueroa-Salcido Oscar Gerardo , Mora-Melgem José Antonio , Granda-Restrepo Diana María , Rodríguez-Bellegarrigue Cecilia Ivonne , Vergara-Jiménez Marcela de Jesús , Cárdenas-Torres Feliznando Isidro , Gracia-Valenzuela Martina Hilda , Cabrera-Chávez Francisco
Published: September 2020

The characteristics of food allergen labelling are relevant for avoiding accidental exposure to the allergens of interest but no Latin American country has evaluated these characteristics. Our aim was to evaluate the characteristics of food allergen labelling and precautionary allergen labelling (PAL) in six Latin American countries. All data were collected directly from the supermarkets surveyed. A total of 10,254 packaged food products were analyzed, of which 63.3% (n = 6494) and 33.2% (n = 3405) featured allergen labelling and/or PAL, respectively. Most products complied with local regulations (≥87.4% for both locally produced and imported). Thirty-three types of PAL statements were detected; the most frequent was “may contain traces of…” (35.1%). Countries without regulations on the characteristics of allergen labelling had two-fold more products that contained allergens in their ingredients lists but no food allergen labelling. The use of PAL in countries that regulate it (38.2%) was as high as that in countries without PAL regulations (19.2%–44.7%). The findings suggest that the lack of regulations for the characteristics of allergen labeling increases the risk of accidental exposure to allergens of interest. Our findings also suggest that beyond regulations, a scientific approach is required for minimizing and standardizing the use of PAL.
Dietary Restrictions and Depressive Symptoms: Longitudinal Results from the Constances Cohort
Authors: Matta Joane , Hoertel Nicolas , Airagnes Guillaume , Czernichow Sebastien , Kesse-Guyot Emmanuelle , Limosin Frederic , Goldberg Marcel , Zins Marie , Lemogne Cédric
Published: September 2020

Cross-sectional results have suggested a linear association between the number of dietary exclusions and depressive symptoms. This longitudinal study aimed to examine the direction of this association. Methods: In the population-based Constances cohort, depressive symptoms were defined by a score ≥19 on the Centre of Epidemiologic Studies-Depression (CES-D) scale. Diet was measured with a 24-item qualitative food frequency questionnaire (FFQ). Both variables were available at inclusion (from 2012 to 2014) and on follow-up (2015 for the CES-D and 2017 for diet). Food exclusion was categorized into five different groups: No exclusion, exclusion of one, two, three, or ≥4 food groups according to the self-reported number of food groups rarely or never consumed. Logistic regressions were conducted, either taking depressive symptoms as the outcome on follow-up with dietary exclusions at baseline as predictor or with the opposite, adjusting for age, sex, education, income, alcohol intake, smoking, physical activity, and anemia. The path analysis included outcomes and covariates in one model. Results: The median follow-up was three years. A total of 29,337 participants (53.4% women, 48.15 ± 12.9 y.o.) had complete CES-D data and 25,356 (53.56% women, 49.05 ± 12.8 y.o.) FFQ data. Dietary exclusion at inclusion predicted depressive symptoms at follow-up (odds ratio [95% confidence interval]: 2.35 [1.62–3.40] for ≥4 excluded items compared to no exclusions). Depressive symptoms at inclusion predicted dietary exclusions at follow-up (3.45 [1.93–6.16] for ≥4 excluded items). In the path analysis, the standardized estimate of the association between dietary exclusions at inclusion and depressive symptoms at follow-up was by far higher than the opposite (0.1863 and 0.00189, respectively, both p < 0.05). Conclusions: The association of dietary exclusion with subsequent depression is stronger than the opposite association.
Effects of Sweet-Liking on Body Composition Depend on Age and Lifestyle: A Challenge to the Simple Sweet-Liking—Obesity Hypothesis
Authors: Iatridi Vasiliki , Armitage Rhiannon M. , Yeomans Martin R. , Hayes John E.
Published: September 2020

Taste hedonics drive food choices, and food choices affect weight maintenance. Despite this, the idea that hyper-palatability of sweet foods is linked to obesity development has been controversial for decades. Here, we investigate whether interpersonal differences in sweet-liking are related to body composition. Healthy adults aged 18–34 years from the UK (n = 148) and the US (n = 126) completed laboratory-based sensory tests (sucrose taste tests) and anthropometric measures (body mass index; BMI, body fat; fat-free mass; FFM, waist/hips circumferences). Habitual beverage intake and lifestyle and behavioural characteristics were also assessed. Using hierarchical cluster analysis, we classified participants into three phenotypes: sweet liker (SL), sweet disliker (SD), and inverted-U (liking for moderate sweetness). Being a SD was linked to higher body fat among those younger than 21 years old, while in the older group, SLs had the highest BMI and FFM; age groups reflected different levels of exposure to the obesogenic environment. FFM emerged as a better predictor of sweet-liking than BMI and body fat. In the older group, sweetened beverage intake partially explained the phenotype–anthropometry associations. Collectively, our findings implicate underlying energy needs as an explanation for the variation in sweet-liking; the moderating roles of age and obesogenic environment require additional consideration.
Risk Reduction for End-Stage Renal Disease by Dietary Guidance Using the Gustatory Threshold Test for Salty Taste
Authors: Ota Yuki , Kitamura Mineaki , Tsuji Kiyokazu , Torigoe Kenta , Yamashita Ayuko , Abe Shinichi , Muta Kumiko , Uramatsu Tadashi , Obata Yoko , Furutani Junya , Takashima Miwa , Mukae Hiroshi , Nishino Tomoya
Published: September 2020

Educational hospitalization of patients with chronic kidney disease (CKD) may slow the progression of renal dysfunction. However, the educational aspect that is more effective has not been identified to date. In this study, patients with CKD were evaluated for gustatory threshold for salty taste and received augmented salt reduction guidance under educational hospitalization at Nagasaki University Hospital from October 2016. In total, 277 eligible patients were enrolled and hospitalized from 2012 to 2019 (mean age of 69.2 years; men comprised 62.1%). We compared 141 patients (Group A) who were educated in the hospital after October 2016 and 136 patients (Group B) who received standard education in the hospital before October 2016. The changes in the estimated glomerular filtration rate (ΔeGFR) after hospitalization and dialysis induction rate within one year after hospitalization were evaluated. The ΔeGFR was significantly improved in Group A compared to Group B (A: 1.05 mL/min/1.73 m2/month, B: 0.55 mL/min/1.73 m2/month; p = 0.02). The dialysis induction rate was significantly lower in Group A than in Group B (A: 8.5%, B: 15.5%; p = 0.001). These trends were also observed by multivariate analyses. In conclusion, educational hospitalization with enhanced salt reduction guidance may reduce the risk of end-stage renal disease.
The Impact of the Use of Glycomacropeptide on Satiety and Dietary Intake in Phenylketonuria
Authors: Daly Anne , Evans Sharon , Pinto Alex , Jackson Richard , Ashmore Catherine , Rocha Júlio César , MacDonald Anita
Published: September 2020

Protein is the most satiating macronutrient, increasing secretion of gastrointestinal hormones and diet induced thermogenesis. In phenylketonuria (PKU), natural protein is restricted with approximately 80% of intake supplied by a synthetic protein source, which may alter satiety response. Casein glycomacropeptide (CGMP-AA), a carbohydrate containing peptide and alternative protein substitute to amino acids (AA), may enhance satiety mediated by its bioactive properties. Aim: In a three-year longitudinal; prospective study, the effect of AA and two different amounts of CGMP-AA (CGMP-AA only (CGMP100) and a combination of CGMP-AA and AA (CGMP50) on satiety, weight and body mass index (BMI) were compared. Methods: 48 children with PKU completed the study. Median ages of children were: CGMP100; (n = 13), 9.2 years; CGMP50; (n = 16), 7.3 years; and AA (n = 19), 11.1 years. Semi-quantitative dietary assessments and anthropometry (weight, height and BMI) were measured every three months. Results: The macronutrient contribution to total energy intake from protein, carbohydrate and fat was similar across the groups. Adjusting for age and gender, no differences in energy intake, weight, BMI, incidence of overweight or obesity was apparent between the groups. Conclusion: In this three-year longitudinal study, there was no indication to support a relationship between CGMP and satiety, as evidenced by decreased energy intake, thereby preventing overweight or obesity. Satiety is a complex multi-system process that is not fully understood.
Household Engagement in Both Aquaculture and Horticulture Is Associated with Higher Diet Quality than Either Alone
Authors: Akter Rumana , Yagi Nobuyuki , Sugino Hiroaki , Thilsted Shakuntala H. , Ghosh Shibani , Gurung Sabi , Heneveld Katherine , Shrestha Robin , Webb Patrick
Published: September 2020

The consumption of high-quality diverse diets is crucial for optimal growth, health, and wellbeing. Objective: This study assessed the diet quality of households by their type of engagement in homestead aquaculture and/or horticulture. Socio-demographic determinants of diet quality were also studied. Method: Diet quality was assessed using a nutrient adequacy ratio (NAR), based on the preceding 7 days’ dietary recall at the household level. Adult male equivalent units (AMEs) were used for age- and sex-specific intra-household distribution of household intakes. Mean adequacy ratios (MAR) were computed as an overall measure of diet quality, using NAR. Results: Better diet quality (mean ± SD) was associated with households engaged in both homestead aquaculture and horticulture (0.43 ± 0.23; p < 0.001) compared to only one type of agriculture (0.38 ± 0.20) or none (0.36 ± 0.20). Tukey’s post-hoc test confirmed significant differences in diet quality between both and either engagement (0.05 ± 0.01, p < 0.001), both and no engagement (0.07 ± 0.01, p < 0.001), and either and no engagement households (0.02 ± 0.01, p < 0.001). Beyond farm production of nutrient-rich foods, generalized estimating equations showed that diet quality was influenced by the higher educational level and occupation of adult household members, higher daily per capita food expenditure, sex, family size and region. Conclusions: Projects that promote and support household engagement in both homestead aquaculture and horticulture have the potential to improve the diet quality of households.
Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells
Authors: Bisht Akshay , Dickens Martin , Rutherfurd-Markwick Kay , Thota Rohith , Mutukumira Anthony N. , Singh Harjinder
Published: September 2020

The anti-inflammatory effects of curcumin are well documented. However, the bioavailability of curcumin is a major barrier to its biological efficacy. Low-dose combination of complimentary bioactives appears to be an attractive strategy for limiting barriers to efficacy of bioactive compounds. In this study, the anti-inflammatory potential of curcumin in combination with chlorogenic acid (CGA), was investigated using human THP-1 macrophages stimulated with lipopolysaccharide (LPS). Curcumin alone suppressed TNF-α production in a dose-dependent manner with a decrease in cell viability at higher doses. Although treatment with CGA alone had no effect on TNF-α production, it however enhanced cell viability and co-administration with curcumin at a 1:1 ratio caused a synergistic reduction in TNF-α production with no impact on cell viability. Furthermore, an qRT-PCR analysis of NF-κB pathway components and inflammatory biomarkers indicated that CGA alone was not effective in reducing the mRNA expression of any of the tested inflammatory marker genes, except TLR-4. However, co-administration of CGA with curcumin, potentiated the anti-inflammatory effects of curcumin. Curcumin and CGA together reduced the mRNA expression of pro-inflammatory cytokines [TNF-α (~88%) and IL-6 (~99%)], and COX-2 (~92%), possibly by suppression of NF-κB (~78%), IκB-β-kinase (~60%) and TLR-4 receptor (~72%) at the mRNA level. Overall, co-administration with CGA improved the inflammation-lowering effects of curcumin in THP-1 cells.
Effects of Indigenous Diet Iron Content and Location on Hemoglobin Levels of Ghanaians
Authors: Callister April , Gautney Joanna , Aguilar Christina , Chan Julian , Aguilar David
Published: September 2020

Multiple studies have demonstrated strong links between diet and anemia, but few have explored the impact of food groups on hemoglobin (Hb). We analyzed the correlation between Ghanaian diet and Hb levels to explore reduction of anemia prevalence through dietary interventions. Demographics, food frequency questionnaires (FFQ), and blood samples were obtained from 140 volunteers (ages 18–65) in five locations across Ghana. Hb was measured; estimated iron consumption was calculated. FFQ items were grouped by food type, and a regression analysis was performed to determine the most important dietary predictors of Hb. Moreover, 47% of total participants were anemic; 64% of females and 28% of males. Hb levels were highest in Mole (13.9 g/dL, SD = ±1.9), independent of sex distribution. The regression model revealed a 62.7% adjusted correlation between food groups and Hb levels. Animal foods (β = 0.016, t = 5.08, p < 0.01) and plant protein (β = 0.013, t = 2.86, p < 0.01) were the most influential groups to Hb levels. It is of vital importance to emphasize the benefits of consuming animal foods and plant proteins within the Ghanaian population. The ease of access to plant proteins makes it likely that this food group will be most influential and have the greatest impact in reduction of anemia in the Ghanaian population.
A Humanities-Based Explanation for the Effects of Emotional Eating and Perceived Stress on Food Choice Motives during the COVID-19 Pandemic
Authors: Shen Wan , Long Lucy M. , Shih Chia-Hao , Ludy Mary-Jon
Published: September 2020

Perceived stress affects emotional eating and food choices. However, the extent to which stress associates with food choice motives is not completely understood. This study assessed whether emotional eating mediates the associations between perceived stress levels and food choice motives (i.e., health, mood, convenience, natural content, price, sensory appeal, familiarities, weight control, and ethical concerns) during the Coronavirus Disease 2019 pandemic. A total of 800 respondents were surveyed in the United States in June 2020. Their perceived stress, emotional eating, and food choice motives were assessed by the Perceived Stress Scale, Dutch Eating Behavior Questionnaire, and Food Choice Questionnaire, respectively. Moderate to high levels of perceived stress were experienced by the majority (73.6%) of respondents. Perceived stress was significantly correlated with emotional eating (r = 0.26) as well as five out of nine food choice motives: mood (r = 0.32), convenience (r = 0.28), natural content (r = −0.14), price (r = 0.27), and familiarity (r = 0.15). Emotional eating was significantly correlated with four out of nine food choice motives: mood (r = 0.27), convenience (r = 0.23), price (r = 0.16), and familiarity (r = 0.16). The mediation analyses showed that emotional eating mediates the associations between perceived stress and five food choices motives: mood, convenience, sensory appeal, price, and familiarity. Findings were interpreted using theories and concepts from the humanities, specifically, folklore studies, ritual studies, and symbolic anthropology.
Molecular Mechanisms of Immune Escape for Foot-and-Mouth Disease Virus
Authors: Yang Bo , Zhang Xiaohui , Zhang Dajun , Hou Jing , Xu GuoWei , Sheng Chaochao , Choudhury Sk Mohiuddin , Zhu Zixiang , Li Dan , Zhang Keshan , Zheng Haixue , Liu Xiangtao
Published: September 2020

Foot-and-mouth disease virus (FMDV) causes a highly contagious vesicular disease in cloven-hoofed livestock that results in severe consequences for international trade, posing a great economic threat to agriculture. The FMDV infection antagonizes the host immune responses via different signaling pathways to achieve immune escape. Strategies to escape the cell immune system are key to effective infection and pathogenesis. This review is focused on summarizing the recent advances to understand how the proteins encoded by FMDV antagonize the host innate and adaptive immune responses.
Applied Barcoding: The Practicalities of DNA Testing for Herbals
Authors: Howard Caroline , Lockie-Williams Claire , Slater Adrian
Published: September 2020

DNA barcoding is a widely accepted technique for the identification of plant materials, and its application to the authentication of commercial medicinal plants has attracted significant attention. The incorporation of DNA-based technologies into the quality testing protocols of international pharmacopoeias represents a step-change in status, requiring the establishment of standardized, reliable and reproducible methods. The process by which this can be achieved for any herbal medicine is described, using Hypericum perforatum L. (St John’s Wort) and potential adulterant Hypericum species as a case study. A range of practical issues are considered including quality control of DNA sequences from public repositories and the construction of individual curated databases, choice of DNA barcode region(s) and the identification of informative polymorphic nucleotide sequences. A decision tree informs the structure of the manuscript and provides a template to guide the development of future DNA barcode tests for herbals.
Correlation between the Crosslink Characteristics and Mechanical Properties of Natural Rubber Compound via Accelerators and Reinforcement
Authors: Kim Do Young , Park Jae Woo , Lee Dong Yun , Seo Kwan Ho
Published: September 2020

The extreme elasticity and reversible deformability of rubber, which is one of the most versatile polymers in modern society, is dependent on several factors, including the processing conditions, curing system, and types of additives used. Since the rubber’s mechanical properties are influenced by the existing structural crosslinks, their correlation with the crosslink characteristics of rubber was investigated using the equilibrium swelling theory of the Flory–Rehner equation and the rubber–filler interaction theory of the Kraus equation. Herein, we examined whether the accelerator and reinforcement agent quantitatively contributed to chemical cross-linkages and rubber–filler interaction. In conclusion, the accelerator content supported the chemically crosslinked structures of the monosulfides and the disulfides in natural rubber (NR). Additionally, these results demonstrated that the mechanical properties and the thermal resistance of NR were dependent on the crosslink characteristics. The findings of this study provide an insight into the development and application of NR products for the mechanical optimization of rubber-based products.
Low-Density Polybutylene Terephthalate Foams with Enhanced Compressive Strength via a Reactive-Extrusion Process
Authors: Aksit Merve , Gröschel Sebastian , Kuhn Ute , Aksit Alper , Kreger Klaus , Schmidt Hans-Werner , Altstädt Volker
Published: September 2020

Due to their appealing properties such as high-temperature dimensional stability, chemical resistance, compressive strength and recyclability, new-generation foams based on engineering thermoplastics such as polyethylene terephthalate (PET) and polybutylene terephthalate (PBT) have been gaining significant attention. Achieving low-density foams without sacrificing the mechanical properties is of vital importance for applications in the field of transportation and construction, where sufficient compressive strength is desired. In contrast to numerous research studies on PET foams, only a limited number of studies on PBT foams and in particular, on extruded PBT foams are known. Here we present a novel route to extruded PBT foams with densities as low as 80 kg/m3 and simultaneously with improved compressive properties manufactured by a tandem reactive-extrusion process. Improved rheological properties and therefore process stability were achieved using two selected 1,3,5-benzene-trisamides (BTA1 and BTA2), which are able to form supramolecular nanofibers in the PBT melt upon cooling. With only 0.08 wt % of BTA1 and 0.02 wt % of BTA2 the normalized compressive strength was increased by 28% and 15%, respectively. This improvement is assigned to the intrinsic reinforcing effect of BTA fibers in the cell walls and struts.
Technical Route to Achieve Ultra-Low Emission of Nitrogen Oxides with Predictive Model of Nitrogen Oxide Background Concentration
Authors: Yao Yanfei , Chen Yanxin
Published: September 2020

As the most mature denitration technology in the cement clinker burning process, selective non-catalytic reduction (SNCR) has been unable to meet the requirements of ultra-low nitrogen oxide (NOX) emissions under low ammonia escape, thus a hybrid denitration process based on SNCR was established. The process had three steps: reducing the NOX background concentration (NBC), implementing staged combustion, and optimizing the effect of the SNCR. One of the keys to this process was the real-time acquisition of the NBC. In this paper, a multivariate linear regression model for the prediction of NBC was constructed and applied to one 12,000 t/d production line and one 5000 t/d production line. For the 12,000 t/d production line, NBC had a positive correlation with the temperature of the calciner outlet, the pressure, and the temperature of the kiln hood, and it had a negative correlation with the quantity of the kiln coal, the temperature of the smoke chamber, and the main motor current of the kiln. The influence degree of each parameter on the NBC is gradually weakened according to the above order. The determination coefficient (R2) of the model was 0.771, and the mean absolute error and maximum relative error between the predicted and measured NBC were 6.300 mg/m3 and 18.670 mg/m3 respectively.
Characterizing the Urban Mine—Simulation-Based Optimization of Sampling Approaches for Built-in Batteries in WEEE
Authors: Mählitz Paul Martin , Korf Nathalie , Sperlich Kristine , Münch Olivier , Rösslein Matthias , Rotter Vera Susanne
Published: September 2020

Comprehensive knowledge of built-in batteries in waste electrical and electronic equipment (WEEE) is required for sound and save WEEE management. However, representative sampling is challenging due to the constantly changing composition of WEEE flows and battery systems. Necessary knowledge, such as methodologically uniform procedures and recommendations for the determination of minimum sample sizes (MSS) for representative results, is missing. The direct consequences are increased sampling efforts, lack of quality-assured data, gaps in the monitoring of battery losses in complementary flows, and impeded quality control of depollution during WEEE treatment. In this study, we provide detailed data sets on built-in batteries in WEEE and propose a non-parametric approach (NPA) to determine MSS. For the pilot dataset, more than 23 Mg WEEE (6500 devices) were sampled, examined for built-in batteries, and classified according to product-specific keys (UNUkeys and BATTkeys). The results show that 21% of the devices had battery compartments, distributed over almost all UNUkeys considered and that only about every third battery was removed prior to treatment. Moreover, the characterization of battery masses (BM) and battery mass shares (BMS) using descriptive statistical analysis showed that neither product- nor battery-specific characteristics are given and that the assumption of (log-)normally distributed data is not generally applicable. Consequently, parametric approaches (PA) to determine the MSS for representative sampling are prone to be biased. The presented NPA for MSS using data-driven simulation (bootstrapping) shows its applicability despite small sample sizes and inconclusive data distribution. If consistently applied, the method presented can be used to optimize future sampling and thus reduce sampling costs and efforts while increasing data quality.
Refining the Joint 3D Processing of Terrestrial and UAV Images Using Quality Measures
Authors: Farella Elisa Mariarosaria , Torresani Alessandro , Remondino Fabio
Published: September 2020

The paper presents an efficient photogrammetric workflow to improve the 3D reconstruction of scenes surveyed by integrating terrestrial and Unmanned Aerial Vehicle (UAV) images. In the last years, the integration of this kind of images has shown clear advantages for the complete and detailed 3D representation of large and complex scenarios. Nevertheless, their photogrammetric integration often raises several issues in the image orientation and dense 3D reconstruction processes. Noisy and erroneous 3D reconstructions are the typical result of inaccurate orientation results. In this work, we propose an automatic filtering procedure which works at the sparse point cloud level and takes advantage of photogrammetric quality features. The filtering step removes low-quality 3D tie points before refining the image orientation in a new adjustment and generating the final dense point cloud. Our method generalizes to many datasets, as it employs statistical analyses of quality feature distributions to identify suitable filtering thresholds. Reported results show the effectiveness and reliability of the method verified using both internal and external quality checks, as well as visual qualitative comparisons. We made the filtering tool publicly available on GitHub.
Spatial Configuration and Extent Explains the Urban Heat Mitigation Potential due to Green Spaces: Analysis over Addis Ababa, Ethiopia
Authors: Terfa Berhanu Keno , Chen Nengcheng , Zhang Xiang , Niyogi Dev
Published: September 2020

Urban green space (UGS) is considered a mitigative intervention for urban heat. While increasing the UGS coverage is expected to reduce the urban heat, studies on the effects of UGS configuration have produced inconsistent results. To investigate this inconsistency further, this study conducted a multi-spatial and multi-temporal resolution analysis in the Addis Ababa city metropolitan area for assessing the relationship between UGS patterns and land surface temperature (LST). Landsat images were used to generate land cover and LST maps. Regression models were developed to investigate whether controlling for the proportion of the green area (PGS), fragmentation, shape, complexity, and proximity distance can affect surface temperature. Results indicated that the UGS patches with aggregated, regular and simple shapes and connectivity throughout the urban landscape were more effective in decreasing the LST as compared to the fragmented and complicated spatial patterns. This finding highlighted that in addition to increasing the amount of UGS, optimizing the spatial structure of UGS, could be an effective and useful action to mitigate the urban heat island (UHI) impacts. Changing the spatial size had a significant influence on the interconnection between LST and UGS patterns as well. It also noted that the spatial arrangement of UGS was more sensitive to spatial scales than that of its composition. The relationship between the spatial configuration of UGS and LST could be changed when applying different statistical methods. This result underlined the importance of controlling the effects of the share of green spaces when calculating the impacts of the spatial configuration of UGS on LST. Furthermore, the study highlighted that applying different statistical approaches, spatial scale, and coverage of UGS can help determine the effectiveness of the association between LST and UGS patterns. These outcomes provided new insights regarding the inconsistent findings from earlier studies, which might be a result of the different approaches considered. Indeed, these findings are expected to be of help more broadly for city planning and urban heat mitigation.