bluuebunny/crossref_metadata_2025 huggingface dataset:
Prediction of Dimensional Changes of Low-Cost Metal Material Extrusion Fabricated Parts Using Machine Learning Techniques
Authors: Zhang Zhicheng , Femi-Oyetoro James , Fidan Ismail , Ismail Muhammad , Allen Michael
Published: April 2021

Additive manufacturing (AM) is a layer-by-layer manufacturing process. However, its broad adoption is still hindered by limited material options, different fabrication defects, and inconsistent part quality. Material extrusion (ME) is one of the most widely used AM technologies, and, hence, is adopted in this research. Low-cost metal ME is a new AM technology used to fabricate metal composite parts using sintered metal infused filament material. Since the involved materials and process are relatively new, there is a need to investigate the dimensional accuracy of ME fabricated metal parts for real-world applications. Each step of the manufacturing process, from the material extrusion to sintering, might significantly affect the dimensional accuracy. This research provides a comprehensive analysis of dimensional changes of metal samples fabricated by the ME and sintering process, using statistical and machine learning algorithms. Machine learning (ML) methods can be used to assist researchers in sophisticated pre-manufacturing planning and product quality assessment and control. This study compares linear regression to neural networks in assessing and predicting the dimensional changes of ME-made components after 3D printing and sintering process. In this research, the ML algorithms present a significantly high coefficient of determination (i.e., 0.999) and a very low mean square error (i.e., 0.0000878). The prediction outcomes using a neural network approach have the smallest mean square error among all ML algorithms and it has quite small p-values. So, in this research, the neural network algorithm has the highest accuracy. The findings of this study can help researchers and engineers to predict the dimensional variations and optimize the printing and sintering process parameters to obtain high quality metal parts fabricated by the low-cost ME process.
Dealing with the Fracture Ductile-to-Brittle Transition Zone of Ferritic Steels Containing Notches: On the Applicability of the Master Curve
Authors: Cicero Sergio , Arrieta Sergio
Published: April 2021

Characterizing the fracture resistance of ferritic steels operating within their Ductile-to-Brittle Transition Zone (DBTZ) has been successfully addressed through the development of the well-known Master Curve (MC). This tool assumes that fracture, in the presence of crack-like defects, is controlled by weakest-link statistics and follows a three-parameter Weibull distribution. When dealing with notch-type defects, there is no standardized solution to predict the fracture resistance within the DBTZ, but the authors have published some works demonstrating that the MC can also be applied in different ways to characterize ferritic steels containing notches. One of these ways is the direct application of the MC methodology, providing a specific reference temperature (T0N) for each material and notch radius. This work reviews this initial attempt to apply the MC in notched conditions, assessing the validity of the main MC hypotheses (initially valid for cracked conditions) when analyzing notch-type defects and providing experimental validation on steels S275JR, S355J2, S460M and S690Q.
Fabrication of a TiC-Ti Matrix Composite Coating Using Ultrasonic Vibration-Assisted Laser Directed Energy Deposition: The Effects of Ultrasonic Vibration and TiC Content
Authors: Li Yunze , Zhang Dongzhe , Wang Hui , Cong Weilong
Published: April 2021

Titanium and its alloys exhibit superior properties of high corrosion resistance, an excellent strength to weight ratio and outstanding stiffness among other things. However, their relatively low hardness and wear resistance limit their service life in high-performance applications of structure parts, gears and bearings, for example. The fabrication of a ceramic reinforced titanium matrix composite (TMC) coating could be one of the solutions to enhance the microhardness and wear resistance. Titanium carbide (TiC) is a preferable candidate due to the advantages of self-lubrication, low cost and a similar density and thermal expansion coefficient with titanium. The fabrication of TiC-TMC coatings onto titanium using a laser directed energy deposition (LDED) process has been conducted. The problems of TiC aggregation, low bonding quality and the generation of fabrication defects still exist. Considering ultrasonic vibration could generate acoustic steaming and transient cavitation actions in melted materials, which could homogenize the distribution of reinforcement materials and promote the dissolution of TiC into liquid titanium. In this study, for the first time, we investigate the ultrasonic vibration-assisted LDED of TiC-TMC coatings. The effects of ultrasonic vibration and reinforcement content on the phase compositions, reinforcement aggregation, bonding quality, fabrication defects and mechanical properties (including microhardness and wear resistance) of LDED deposited TiC-TMC coatings have been investigated. With the assistance of ultrasonic vibration, the aggregation of TiC was reduced, the porosity was decreased, the defects in the bonding interface were reduced and the mechanical properties including microhardness and wear resistance were increased. However, the excessive TiC content could significantly increase the TiC aggregation and manufacturing defects, resulting in the reduction of the mechanical properties.
Analog Spatial Light Modulators Based on Micromirror Arrays
Authors: Dauderstädt Ulrike , Dürr Peter , Gehner Andreas , Wagner Michael , Schenk Harald
Published: April 2021

The Fraunhofer Institute for Photonic Microsystems (IPMS) has been developing and manufacturing micromirror arrays for more than 20 years. While originally focusing on applications related to microlithography and therefore mainly for light in the deep ultraviolet range, the range of applications has been expanded since, including applications in the visible and near-infrared range. This paper gives an overview of the devices and their designs, fabrication, and characterization.
Influence of High-Intensity Focused Ultrasound (HIFU) Ablation on Arteries: Ex Vivo Studies
Authors: Zhou Yufeng , Lim Wei Chun Daniel
Published: April 2021

High-intensity focused ultrasound (HIFU) has been used to ablate solid tumors and cancers. Because of the hypervascular structure of the tumor and circulating blood inside it, the interaction between the HIFU burst and vessel is a critical issue in the clinical environment. Influences on lesion production and the potential of vessel rupture were investigated in this study for the efficiency and safety of clinical ablation. An extracted porcine artery was embedded in a transparent polyacrylamide gel phantom, with bovine serum albumin (BSA) as an indicator of the thermal lesion, and degassed water was driven through the artery sample. The HIFU focus was aligned to the anterior wall, middle of the artery, and posterior wall. After HIFU ablation, the produced lesion was photographically recorded, and then its size was quantified and compared with that in the gel phantom without artery. In addition, the bubble dynamics (i.e., generation, expansion, motion, and shrinkage of bubbles and their interaction with the artery) were captured using high-speed imaging. It was found that the presence of the artery resulted in a decrease in lesion size in both the axial and lateral directions. The characteristics of the lesion are dependent on the focus alignment. Acoustic and hydrodynamic cavitation play important roles in lesion production and interaction with the artery. Both thermal and mechanical effects were found on the surface of the artery wall after HIFU ablation. However, no vessel rupture was found in this ex vivo study.
Development of Quantum Dot (QD) Based Color Converters for Multicolor Display
Authors: Sajjad Muhammad T. , Bansal Ashu K. , Antolini Francesco , Preis Eduard , Stroea Lenuta , Toffanin Stefano , Muccini Michele , Ortolani Luca , Migliori Andrea , Allard Sybille , Scherf Ullrich , Samuel Ifor D. W.
Published: April 2021

Many displays involve the use of color conversion layers. QDs are attractive candidates as color converters because of their easy processability, tuneable optical properties, high photoluminescence quantum yield, and good stability. Here, we show that emissive QDs with narrow emission range can be made in-situ in a polymer matrix, with properties useful for color conversion. This was achieved by blending the blue-emitting pyridine based polymer with a cadmium selenide precursor and baking their films at different temperatures. To achieve efficient color conversion, blend ratio and baking temperature/time were varied. We found that thermal decomposition of the precursor leads to highly emissive QDs whose final size and emission can be controlled using baking temperature/time. The formation of the QDs inside the polymer matrix was confirmed through morphological studies using atomic force microscopy (AFM) and transmission electron microscopy (TEM). Hence, our approach provides a cost-effective route to making highly emissive color converters for multi-color displays.
Thermoelectric Energy Harvesting from Single-Walled Carbon Nanotube Alkali-Activated Nanocomposites Produced from Industrial Waste Materials
Authors: Davoodabadi Maliheh , Vareli Ioanna , Liebscher Marco , Tzounis Lazaros , Sgarzi Massimo , Paipetis Alkiviadis , Yang Jian , Cuniberti Gianaurelio , Mechtcherine Viktor
Published: April 2021

A waste-originated one-part alkali-activated nanocomposite is introduced herein as a novel thermoelectric material. For this purpose, single-walled carbon nanotubes (SWCNTs) were utilized as nanoinclusions to create an electrically conductive network within the investigated alkali-activated construction material. Thermoelectric and microstructure characteristics of SWCNT-alkali-activated nanocomposites were assessed after 28 days. Nanocomposites with 1.0 wt.% SWCNTs exhibited a multifunctional behavior, a combination of structural load-bearing, electrical conductivity, and thermoelectric response. These nanocomposites (1.0 wt.%) achieved the highest thermoelectric performance in terms of power factor (PF), compared to the lower SWCNTs’ incorporations, namely 0.1 and 0.5 wt.%. The measured electrical conductivity (σ) and Seebeck coefficient (S) were 1660 S·m−1 and 15.8 µV·K−1, respectively, which led to a power factor of 0.414 μW·m−1·K−2. Consequently, they have been utilized as the building block of a thermoelectric generator (TEG) device, which demonstrated a maximum power output (Pout) of 0.695 µW, with a power density (PD) of 372 nW·m−2, upon exposure to a temperature gradient of 60 K. The presented SWCNT-alkali-activated nanocomposites could establish the pathway towards waste thermal energy harvesting and future sustainable civil engineering structures.
Formyl Peptide Receptor (FPR)1 Modulation by Resveratrol in an LPS-Induced Neuroinflammatory Animal Model
Authors: Calvello Rosa , Cianciulli Antonia , Porro Chiara , Moda Piergianni , De Nuccio Francesco , Nicolardi Giuseppe , Giannotti Laura , Panaro Maria Antonietta , Lofrumento Dario Domenico
Published: April 2021

Among therapeutic approaches that have been investigated, targeting of receptors implicated in managing neuroinflammation has been described. One such family of receptors comprises the formyl peptide receptors (FPRs) whose ligands could play a role in host defense. The murine FPR gene family includes at least six members while in humans there are only three. The two most important members are the Fpr1 and Fpr2. Fpr1encodes murine FPR1, which is considered the murine orthologue of human FPR. Resveratrol, a non-flavonoid polyphenol rich in red wine and grapes, apart from its beneficial health effects and anti-inflammatory properties, has been reported to reduce neuroinflammation in different neurodegenerative disease models. Resveratrol anti-inflammatory responses involve the activation of the protein deacetylase sirtuin 1 (SIRT1) gene. In this work we have investigated in an LPS-based murine model of neuroinflammation the role of FPR1, examining not only if this receptor undergoes a reduction of its expression during neuroinflammation, but also whether treatment with resveratrol was able to modulate its expression leading to an amelioration of neuroinflammatory picture in a murine model of neuroinflammation. Results of this work showed that FPR1 together with SIRT1 resulted upregulated by resveratrol treatment and that this increase is associated with an amelioration of the neuroinflammatory picture, as demonstrated by the induction of IL-10 and IL1-RA expression and the downregulation of proinflammatory mediators, such as TNF-α and IL-1β. The expression and the modulation of FPR1 by resveratrol may be evaluated in order to propose a novel anti-inflammatory and pro-resolving therapeutic approach for the reduction of the detrimental effects associated with neuro-inflammation based neurodegenerative diseases and also as a promising strategy to promote human health by a diet rich in antioxidative bioactive compounds.
Seafood Intake as a Method of Non-Communicable Diseases (NCD) Prevention in Adults
Authors: Jamioł-Milc Dominika , Biernawska Jowita , Liput Magdalena , Stachowska Laura , Domiszewski Zdzisław
Published: April 2021

Seafood (fish in particular) is one of the main food groups in nutrition models with proven health benefits. Seafood has long been considered a very valuable dietary component, mainly due to presence of n-3 polyunsaturated fatty acids (n-3 PUFA) but it is also an important source of protein (including collagen), anserine, taurine, iodine, selenium, vitamin A, vitamin K, vitamin D, tocopherols, B vitamins and astaxanthin. Considering the beneficial effects of these ingredients on blood pressure, lipid profile and the inflammatory process, seafood should be an essential component of the diet. Non-communicable diseases (NCD) such as cardiovascular diseases, cancer, diabetes and mental disorder, chronic respiratory diseases are common diseases associated with advanced age. Promotion of a healthy lifestyle (including proper nutritional behavior) and prevention of diseases are the most effective and efficient ways to decrease premature mortality from NCD and to maintain mental health and well-being. This review article shows the potential preventive and therapeutic effects of seafood with an emphasis on fish. Our narrative review presents the results of systematic reviews and meta-analysis.
Anti-Inflammatory and Anti-Oxidative Synergistic Effect of Vitamin D and Nutritional Complex on Retinal Pigment Epithelial and Endothelial Cell Lines against Age-Related Macular Degeneration
Authors: Hernandez Maria , Recalde Sergio , González-Zamora Jorge , Bilbao-Malavé Valentina , Sáenz de Viteri Manuel , Bezunartea Jaione , Moreno-Orduña Maite , Belza Idoia , Barrio-Barrio Jesús , Fernandez-Robredo Patricia , García-Layana Alfredo
Published: April 2021

Age-related macular degeneration (AMD) is a multifactorial disease of the retina featured by dysfunction of retinal pigmented epithelial (RPE) and loss of photoreceptor cells under oxidative stress and inflammatory conditions. Vitamin D and antioxidants have beneficial effects against retinal degenerative diseases, such as AMD. We investigated the impact of associating vitamin D (ND) with a nutritional antioxidant complex (Nutrof Total®; N) on oxidative stress and inflammation-like induced conditions by H2O2 and LPS, respectively, in human retinal epithelial (ARPE-19) and human retinal endothelial (HREC) cells. Application of either N or ND treatments to H2O2-induced media in ARPE-19 cells counteracted late apoptosis, attenuated oxidative DNA damage, and increased cell proliferation. Significant reduction in the expression levels of MCP1, IL-8, and IL6 cytokines was observed following application of either N or ND treatments under LPS-induced conditions in ARPE-19 cells and in MCP-1 and IL12p70 cytokine levels in HREC cells. ND and not N revealed significant downregulation of IFNγ in ARPE-19 cells, and of IL-6 and IL-18 in HREC cells. In conclusion, adding vitamin D to Nutrof Total® protects in a synergistic way against oxidative and inflammatory stress-induced conditions in retinal epithelial and endothelial cells.
In Vivo Evaluation of Dendropanax morbifera Leaf Extract for Anti-Obesity and Cholesterol-Lowering Activity in Mice
Authors: Song Ji-Hye , Kim Hyunhee , Jeong Minseok , Kong Min Jung , Choi Hyo-Kyoung , Jun Woojin , Kim Yongjae , Choi Kyung-Chul
Published: April 2021

Metabolic syndrome is a worldwide health problem, and obesity is closely related to type 2 diabetes, cardiovascular disease, hypertension, and cancer. According to WHO in 2018, the prevalence of obesity in 2016 tripled compared to 1975. D. morbifera reduces bad cholesterol and triglycerides levels in the blood and provides various antioxidant nutrients and germicidal sub-stances, as well as selenium, which helps to remove active oxygen. Moreover, D. morbifera is useful for treating cardiovascular diseases, hypertension, hyperlipidemia, and diabetes. Therefore, we study in vivo efficacy of D. morbifera to investigate the prevention effect of obesity and cholesterol. The weight and body fat were effectively reduced by D. morbifera water (DLW) extract administration to high-fat diet-fed C57BL/6 mice compared to those of control mice. The group treated with DLW 500 mg∙kg−1∙d−1 had significantly lower body weights compared to the control group. In addition, High-density lipoprotein (HDL) cholesterol increased in the group treated with DLW 500 mg∙kg−1∙d−1. The effect of DLW on the serum lipid profile could be helpful to prevent obesity. DLW suppresses lipid formation in adipocytes and decreases body fat. In conclusion, DLW can be applied to develop anti-obesity functional foods and other products to reduce body fat.
Improvement in Eating Disorder Risk and Psychological Health in People with Class 3 Obesity: Effects of a Multidisciplinary Weight Management Program
Authors: Piya Milan K. , Chimoriya Ritesh , Yu William , Grudzinskas Kathy , Myint Kyaw Phone , Skelsey Kathryn , Kormas Nic , Hay Phillipa
Published: April 2021

This study aimed to evaluate the risk of eating disorders, psychological distress, and health-related quality of life (HRQoL) in people with class 3 obesity (body mass index (BMI) ≥ 40 kg/m2), and the effect of multidisciplinary weight management over 12 months. This retrospective cohort study included all adults with class 3 obesity who enrolled in a weight management program from March 2018 to December 2019. Questionnaires included the Eating Disorder Examination Questionnaire Short (EDE-QS), Kessler Psychological Distress Scale (K10), and 36-Item Short Form Survey (SF-36) for HRQoL. Physical and Mental Component Summary scores (PCS and MCS) were derived from the SF-36. Of 169 participants who completed 12 months in the program, 65.7% (n = 111) completed questionnaires at baseline and 12 months, with 6.0 ± 6.8% weight loss over this period. Compared to baseline, there was significant improvement at 12 months in EDE-QS (15.7 ± 6.6 vs. 13.6 ± 6.2, p = 0.002), K10 (25.7 ± 9.7 vs. 21.2 ± 9.4, p < 0.001), PCS (29.4 ± 10.1 vs. 36.1 ± 10.9, p < 0.001), and MCS scores (40.2 ± 12.4 vs. 44.0 ± 13.4, p = 0.001). All, apart from EDE-QS scores, remained significant after adjusting for weight change. This study highlights the importance of multidisciplinary management in people with class 3 obesity to help reduce eating disorder risk and psychological distress, and improve HRQoL, in addition to weight loss.
Time-Restricted Eating Improves Quality of Life Measures in Overweight Humans
Authors: Crose Ainslee , Alvear Alison , Singroy Stephanie , Wang Qi , Manoogian Emily , Panda Satchidananda , Mashek Douglas G. , Chow Lisa S.
Published: April 2021

Time-restricted eating (TRE) reduces weight in humans, but its effects on quality of life have not been well characterized. By performing a secondary analysis of a randomized clinical trial, we examined the effects of TRE (12-week intervention, 8 h eating window) vs. non-TRE (unrestricted eating) on quality of life (QoL) measures. Twenty subjects with overweight and prolonged eating window (mean (SD): 15.4 h (0.9)) were randomized to either 12 weeks of TRE (8 h eating window: (n = 11)) or non-TRE (n = 9). QoL data were collected with the 36-item Short Form Survey (SF-36) pre- and post-intervention. Given a two-way ANOVA model and post-hoc t-test analysis, the TRE group improved limitations due to emotional health post-intervention: +97.0 (10.0)) vs. baseline: +66.7 (42.2) (p = 0.02) and perceived change in health over the last year end intervention: +68.2 (16.2) vs. baseline: +52.3 (23.6) (p = 0.001) relative to baseline. The TRE group improved limitations due to emotional health TRE: +97.0 (10.0) vs. non-TRE: +55.6 (44.1) (p = 0.05) and perceived change in health (TRE: +68.2 (16.2) vs. non-TRE: +44.4 (31.6) (p = 0.04) relative to the non-TRE group at post-intervention (p < 0.025). In conclusion, 12 weeks of TRE does not adversely affect QoL and may be associated with modest improvements in QoL relative to baseline and unrestricted eating; these findings support future studies examining TRE compliance and durability.
High-Protein, Low-Glycaemic Meal Replacement Decreases Fasting Insulin and Inflammation Markers—A 12-Month Subanalysis of the ACOORH Trial
Authors: Kempf Kerstin , Röhling Martin , Banzer Winfried , Braumann Klaus , Halle Martin , McCarthy David , Predel Hans , Schenkenberger Isabelle , Tan Susanne , Toplak Hermann , Berg Aloys , Martin Stephan ,   on behalf of ACOORH Study Group
Published: April 2021

Lifestyle interventions, including meal replacement, are effective in the prevention and treatment of type-2-diabetes and obesity. Since insulin is the key weight regulator, we hypothesised that the addition of meal replacement to a lifestyle intervention reduces insulin levels more effectively than lifestyle intervention alone. In the international multicentre randomised controlled ACOORH (Almased Concept against Overweight and Obesity and Related Health Risk) trial, overweight or obese persons who meet the criteria for metabolic syndrome (n = 463) were randomised into two groups. Both groups received nutritional advice focusing on carbohydrate restriction and the use of telemonitoring devices. The intervention group substituted all three main meals per day in week 1, two meals per day in weeks 2–4, and one meal per day in weeks 5–26 with a protein-rich, low-glycaemic meal replacement. Data were collected at baseline and after 1, 3, 6 and 12 months. All datasets providing insulin data (n = 446) were included in this predefined subanalysis. Significantly higher reductions in insulin (−3.3 ± 8.7 µU/mL vs. −1.6 ± 9.8 µU/mL), weight (−6.1 ± 5.2 kg vs. −3.2 ± 4.6 kg), and inflammation markers were observed in the intervention group. Insulin reduction correlated with weight reduction and the highest amount of weight loss (−7.6 ± 4.9 kg) was observed in those participants with an insulin decrease > 2 µU/mL. These results underline the potential for meal replacement-based lifestyle interventions in diabetes prevention, and measurement of insulin levels may serve as an indicator for adherence to carbohydrate restriction.
Elite Male Volleyball Players Are at Risk of Insufficient Energy and Carbohydrate Intake
Authors: Sesbreno Erik , Dziedzic Christine E. , Sygo Jennifer , Blondin Denis P. , Haman François , Leclerc Suzanne , Brazeau Anne-Sophie , Mountjoy Margo
Published: April 2021

Elite volleyball athletes experience significant physical and psychological demands during the competitive season. The aim was to compare the dietary intake of male volleyball athletes with recommendations for sport and health, and to examine the association of physique traits and knee health on eating behaviours and of eating behaviours on reported dietary intake. Using a retrospective cross-sectional design, 22 male athletes from a national indoor volleyball program underwent anthropometric, dual-energy X-ray absorptiometry and resting metabolic rate testing, 4-day dietary intake and hematological analysis, and also completed the three-factor eating questionnaire–R18 for eating behaviours and the Victorian Institute of Sport Assessment—patellar tendon (VISA-P) questionnaire for knee health. Most players under-consumed energy compared to reference guidelines, secondary to under-consuming carbohydrate for exercise. The primary eating behaviour was cognitive restraint, which was associated with body mass index and ectomorphy. Emotional eating behaviour was associated with VISA-P. Differences in emotional and cognitive restraint eating behaviours did not impact dietary intake. The findings suggest that players are at risk of an impaired ability to adapt to and recover from training during an important segment of the competitive season. Future work should explore the presence of low energy availability in elite male volleyball players.
Prebiotic Effect of Berberine and Curcumin Is Associated with the Improvement of Obesity in Mice
Authors: Neyrinck Audrey M. , Sánchez Cándido Robles , Rodriguez Julie , Cani Patrice D. , Bindels Laure B. , Delzenne Nathalie M.
Published: April 2021

Berberine and curcumin, used as food additives or food supplements, possess interesting anti-inflammatory and antioxidant properties. We tested the potential protective effect of both phytochemicals in genetically obese mice and we determined whether these effects can be related to the modulation of gut functions and microbiota. Ob/ob mice were fed a standard diet supplemented with or without 0.1% berberine and/or 0.3% curcumin for 4 weeks. By using targeted qPCR, we found that cecal content of Bifidobacterium spp. and Akkermansia spp. increased mainly upon berberine supplementation. Genes involved in innate immunity (Pla2g2a), mucus production (Muc2) and satietogenic peptide production (Gcg and Pyy) were upregulated in the colon of mice treated with both phytochemicals. Berberine supplementation alone reduced food intake, body weight gain, hypertriglyceridemia and hepatic inflammatory and oxidative stress markers, thus lessening hepatic injury. The increase in Bifidobacterium spp. and Akkermansia spp. was correlated with the improvement of gut barrier function and with the improvement of hepatic inflammatory and oxidative stresses in obese mice. These data support the fact that non-carbohydrate phytochemicals may modulate the gut microbiota in obesity and related gut and hepatic alterations.
Single Ingestion of Trehalose Enhances Prolonged Exercise Performance by Effective Use of Glucose and Lipid in Healthy Men
Authors: Hamada Naomi , Wadazumi Tsuyoshi , Hirata Yoko , Kuriyama Mayumi , Watanabe Kanji , Watanabe Hitoshi , Hongu Nobuko , Arai Norie
Published: April 2021

Trehalose increases blood glucose levels slowly and induces a slight insulin response. The present study aimed to study the effect of trehalose on prolonged exercise performance. The participants were 12 healthy men (age: 21.3 ± 0.9 y). After an overnight fast (12 h), they first exercised with a constant load (intensity: 40% V˙O2peak) for 60 min using a bicycle ergometer. They continued to exercise with a constant load (40% V˙O2peak) for 30 min between four sets of the 30-s Wingate test. After the 1st set, each participant ingested 500 mL water (control), 8% glucose, or 8% trehalose in three trials. These three trials were at least one week apart and were conducted in a double-blind and randomized crossover manner. Blood was collected for seven biochemical parameters at 12 time points during the experiment. The area under the curve of adrenaline after ingestion of trehalose was significantly lower than that for water and tended to be lower than that for glucose in the later stage of the exercise. Lower secretion of adrenaline after a single dose of 8% trehalose during prolonged exercise reflected the preservation of carbohydrates in the body in the later stage of the exercise. In conclusion, a single ingestion of trehalose helped to maintain prolonged exercise performance.
Association between Fruit and Vegetable Intakes and Mental Health in the Australian Diabetes Obesity and Lifestyle Cohort
Authors: Rees Joanna , Radavelli Bagatini Simone , Lo Johnny , Hodgson Jonathan M. , Christophersen Claus T. , Daly Robin M. , Magliano Dianna J. , Shaw Jonathan E. , Sim Marc , Bondonno Catherine P. , Blekkenhorst Lauren C. , Dickson Joanne M. , Lewis Joshua R. , Devine Amanda
Published: April 2021

Increasing prevalence of mental health disorders within the Australian population is a serious public health issue. Adequate intake of fruits and vegetables (FV), dietary fibre (DF) and resistant starch (RS) is associated with better mental and physical health. Few longitudinal studies exist exploring the temporal relationship. Using a validated food frequency questionnaire, we examined baseline FV intakes of 5845 Australian adults from the AusDiab study and estimated food group-derived DF and RS using data from the literature. Perceived mental health was assessed at baseline and 5 year follow up using SF-36 mental component summary scores (MCS). We conducted baseline cross-sectional analysis and prospective analysis of baseline dietary intake with perceived mental health at 5 years. Higher baseline FV and FV-derived DF and RS intakes were associated with better 5 year MCS (p < 0.001). A higher FV intake (754 g/d vs. 251 g/d, Q4 vs. Q1) at baseline had 41% lower odds (OR = 0.59: 95% CI 0.46–0.75) of MCS below population average (<47) at 5 year follow up. Findings were similar for FV-derived DF and RS. An inverse association was observed with discretionary food-derived DF and RS. This demonstrates the association between higher intakes of FV and FV-derived DF and RS with better 5 year mental health outcomes. Further RCTs are necessary to understand mechanisms that underlie this association including elucidation of causal effects.
Physical Activity, Sedentariness, Eating Behaviour and Well-Being during a COVID-19 Lockdown Period in Greek Adolescents
Authors: Morres Ioannis D. , Galanis Evangelos , Hatzigeorgiadis Antonis , Androutsos Odysseas , Theodorakis Yannis
Published: April 2021

Adolescents’ daily life has dramatically changed during the COVID-19 era due to the social restrictions that have been imposed, including closures of schools, leisure centers and sport facilities. The purpose of this study was to examine levels of well-being and mood and their relations with physical (in)activity and eating behaviors in adolescents during a lockdown period in Greece. A total of 950 adolescents (Mean Age = 14.41 years ± 1.63) participated in a web-based survey while education was conducted online and organized sport activities were interrupted. Participants showed poor well-being, insufficient physical activity levels and moderate scores of healthy eating behavior. Hierarchical regression analysis showed that, after controlling for the effect of gender and body mass index, increased physical activity and healthier eating behavior predicted better well-being (b = 0.24, p < 0.01 and b = 0.19, p < 0.01, respectively), whereas sedentariness predicted worse well-being (b = −0.16, p < 0.01). Furthermore, it was revealed that days of physical activity per week was a stronger predictor of well-being than minutes of physical activity per week, and that both in-house and out-of-house physical activity were beneficial. Considering that well-being in our study was below the threshold recommended by the World Health Organization as indicative of possible depressive symptoms, measures to increase physical activity, decrease sedentariness and improve eating behavior should become a priority for communities and policy makers.
Brown Adipose Tissue: New Challenges for Prevention of Childhood Obesity. A Narrative Review
Authors: Verduci Elvira , Calcaterra Valeria , Di Profio Elisabetta , Fiore Giulia , Rey Federica , Magenes Vittoria Carlotta , Todisco Carolina Federica , Carelli Stephana , Zuccotti Gian Vincenzo
Published: April 2021

Pediatric obesity remains a challenge in modern society. Recently, research has focused on the role of the brown adipose tissue (BAT) as a potential target of intervention. In this review, we revised preclinical and clinical works on factors that may promote BAT or browning of white adipose tissue (WAT) from fetal age to adolescence. Maternal lifestyle, type of breastfeeding and healthy microbiota can affect the thermogenic activity of BAT. Environmental factors such as exposure to cold or physical activity also play a role in promoting and activating BAT. Most of the evidence is preclinical, although in clinic there is some evidence on the role of omega-3 PUFAs (EPA and DHA) supplementation on BAT activation. Clinical studies are needed to dissect the early factors and their modulation to allow proper BAT development and functions and to prevent onset of childhood obesity.
Measuring and Leveraging Motives and Values in Dietary Interventions
Authors: Eustis Sarah J. , Turner-McGrievy Gabrielle , Adams Swann A. , Hébert James R.
Published: April 2021

Why measure and leverage food motives and values? Every failure and every success in dietary change can be connected to motivation. Therefore, this research question naturally arises: How can food motives and values be measured and leveraged to improve diet outcomes from the individual to populations? There are four ways that food motives and values (FMVs) can assist researchers and health professionals. First, FMVs can help to create a personalized approach to dietary change. Second, FMVs can inform content for dietary interventions. Third, these FMV measures can be used in data analysis to elucidate differences in adherence and outcomes among participants. Fourth, public health nutrition messages can be tailored using information on FMVs. Each of these uses has the potential to further the literature and inform future efforts to improve diet. A central aim of our study is to provide specific examples and recommendations on how to measure and leverage FMVs. To do so, we reviewed 12 measures included in the literature citing the Food Choice Questionnaire by Steptoe, Pollard, and Wardle, which was identified as the earliest, highly cited article appearing under the search terms “food motives” AND “food values” AND “eating behavior” AND “measure”. Specific details on how articles were selected from the citing literature are described in the Methods section. We also expound on our reasoning for including the Three-Factor Eating Questionnaire, which made for 13 measures in total. Our main finding is that each measure has strengths and shortcomings to consider in using FMVs to inform nutritional recommendations at different levels.
Preliminary Biological Activity Screening of Plectranthus spp. Extracts for the Search of Anticancer Lead Molecules
Authors: Ntungwe Epole , Domínguez-Martín Eva María , Teodósio Catarina , Teixidó-Trujillo Silvia , Armas Capote Natalia , Saraiva Lucilia , Díaz-Lanza Ana María , Duarte Noélia , Rijo Patrícia
Published: April 2021

Plectranthus species (Lamiaceae) have been employed in traditional medicine and this is now validated by the presence of bioactive abietane-type diterpenoids. Herein, sixteen Plectranthus acetonic extracts were prepared by ultrasound-assisted extraction and their biological activity was screened. The antimicrobial activity of each extract was screened against yeasts, and Gram-positive and Gram-negative bacteria. The P. hadiensis and P. mutabilis extracts possessed significant activity against Staphylococcus aureus and Candida albicans (microdilution method). Moreover, all extracts showed antioxidant activity using the DPPH method, with P. hadiensis and P. mutabilis extracts having the highest scavenging activities. Selected by the Artemia salina model, P. hadiensis and P.ciliatus possessed low micromolar anti-proliferative activities in human colon, breast, and lung cancer cell lines. Furthermore, the most bioactive extract of P. hadiensis leaves and the known abietane diterpene, 7α-acetoxy-6β-hydroxyroyleanone isolated from this plant, were tested against the aggressive type triple negative breast cancer (MDA-MB-231S). P. hadiensis extract reduced the viability of MDA-MB-231S cancer cell line cells, showing an IC50 value of 25.6 µg/mL. The IC50 value of 7α-acetoxy-6β-hydroxyroyleanone was 5.5 µM (2.15 µg/mL), suggesting that this lead molecule is a potential starting tool for the development of anti-cancer drugs.
The Relationship between Pulmonary Damage and Peripheral Vascular Manifestations in Systemic Sclerosis Patients
Authors: Ruaro Barbara , Confalonieri Marco , Salton Francesco , Wade Barbara , Baratella Elisa , Geri Pietro , Confalonieri Paola , Kodric Metka , Biolo Marco , Bruni Cosimo
Published: April 2021

Systemic sclerosis (SSc) is an autoimmune disease, characterized by the presence of generalized vasculopathy and tissue fibrosis. Collagen vascular disorder in SSc is due to fibroblast and endothelial cell dysfunctions. This leads to collagen overproduction, vascular impairment and immune system abnormalities and, in the last stage, multi-organ damage. Thus, to avoid organ damage, which has a poor prognosis, all patients should be carefully evaluated and followed. This is particularly important in the initial disease phase, so as to facilitate early identification of any organ involvement and to allow for appropriate therapy. Pulmonary disease in SSc mainly involves interstitial lung disease (ILD) and pulmonary arterial hypertension (PAH). High-resolution computed tomography (HRCT) and pulmonary function tests (PFT) have been proposed to monitor parenchymal damage. Although transthoracic echocardiography is the most commonly used screening tool for PAH in SSc patients, definitive diagnosis necessitates confirmation by right heart catheterization (RHC). Moreover, some studies have demonstrated that nailfold videocapillaroscopy (NVC) provides an accurate evaluation of the microvascular damage in SSc and is able to predict internal organ involvement, such as lung impairment. This review provides an overview of the correlation between lung damage and microvascular involvement in SSc patients.
Supercritical CO2 Extraction of Organic Solvents from Flunisolide and Fluticasone Propionate
Authors: Baldino Lucia , Scognamiglio Mariarosa , Reverchon Ernesto
Published: April 2021

In this work, Class 2 and Class 3 solvents contained in two corticosteroids, flunisolide (Fluni) and fluticasone propionate (Fluti), were reduced to a few ppm by supercritical CO2 extraction. The process was carried out at pressures from 80 to 200 bar, temperatures of 40 °C and 80 °C, and at a fixed CO2 flow rate of 0.7 kg/h. The results demonstrated that CO2 density is the key parameter influencing the extraction kinetics and the solvent final residue. In particular, in the range investigated, optimal pressure and temperature conditions for the extraction of residual organic solvents were found working at 200 bar and 40 °C, which corresponds to a CO2 density of 0.840 g/cm3. Operating in this way, total organic solvent residues were reduced from 13,671 ppm and 326 ppm to 12 ppm and 10 ppm for Fluni and Fluti, respectively.
A Multilayer Functionalized Drug-Eluting Balloon for Treatment of Coronary Artery Disease
Authors: Lee Hak-Il , Rhim Won-Kyu , Kang Eun-Young , Choi Bogyu , Kim Jun-Hyeok , Han Dong-Keun
Published: April 2021

Drug-eluting balloons (DEBs) have been mostly exploited as an interventional remedy for treating atherosclerosis instead of cardiovascular stents. However, the therapeutic efficacy of DEB is limited due to their low drug delivery capability to the disease site. The aim of our study was to load drugs onto a balloon catheter with preventing drug loss during transition time and maximizing drug transfer from the surface of DEBs to the cardiovascular wall. For this, a multilayer-coated balloon catheter, composed of PVP/Drug-loaded liposome/PVP, was suggested. The hydrophilic property of 1st layer, PVP, helps to separate drug layer in hydrophilic blood vessel, and the 2nd layer with Everolimus (EVL)-loaded liposome facilitates drug encapsulation and sustained release to the targeted lesions during inflation time. Additionally, a 3rd layer with PVP can protect the inner layer during transition time for preventing drug loss. The deionized water containing 20% ethanol was utilized to hydrate EVL-loaded liposome for efficient coating processes. The coating materials showed negligible toxicity in the cells and did not induce pro-inflammatory cytokine in human coronary artery smooth muscle cells (HCASMCs), even in case of inflammation induction through LPS. The results of hemocompatibility for coating materials exhibited that protein adsorption and platelet adhesion somewhat decreased with multilayer-coated materials as compared to bare Nylon tubes. The ex vivo experiments to confirm the feasibility of further applications of multilayer-coated strategy as a DEB system demonstrated efficient drug transfer of approximately 65% in the presence of the 1st layer, to the tissue in 60 s after treatment. Taken together, a functional DEB platform with such a multilayer coating approach would be widely utilized for percutaneous coronary intervention (PCI).
Phase Measurement of Guided-Mode Resonance Device Using Digital Micromirror Device Gratings
Authors: Chiang Min-Xu , Tongpakpanang Jaturon , Kuo Wen-Kai
Published: April 2021

This paper reports on the measurement system of the phase difference between s- and p-polarization components of the light passing through a guided-mode resonance (GMR) device using a digital micromirror device (DMD) gratings as a digital phase-shifting device. The phase of the non-zeroth order diffraction beams of the grating pattern displayed on the DMD can exhibit a phase change when the grating pattern is shifted. Two nearest different diffraction orders of p-polarized and s-polarized beams can be used as the reference and measurement beams, respectively, and are combined to implement the phase-shifting interferometry (PSI). The phase difference between the s- and the p-polarization components of the incident light passing through the GMR device can be obtained by applying the four-step phase-shift algorithm to the DMD-based PSI system. Experimental results show that this measurement system has a phase detection limit of 1° and was able to obtain the abrupt phase difference curve of the GMR device versus the incident angle.
Free-Space Nonreciprocal Transmission Based on Nonlinear Coupled Fano Metasurfaces
Authors: Mekawy Ahmed , Sounas Dimitrios L. , Alù Andrea
Published: April 2021

Optical nonlinearities can enable unusual light–matter interactions, with functionalities that would be otherwise inaccessible relying only on linear phenomena. Recently, several studies have harnessed the role of optical nonlinearities to implement nonreciprocal optical devices that do not require an external bias breaking time-reversal symmetry. In this work, we explore the design of a metasurface embedding Kerr nonlinearities to break reciprocity for free-space propagation, requiring limited power levels. After deriving the general design principles, we demonstrate an all-dielectric flat metasurface made of coupled nonlinear Fano silicon resonant layers realizing large asymmetry in optical transmission at telecommunication frequencies. We show that the metrics of our design can go beyond the fundamental limitations on nonreciprocity for nonlinear optical devices based on a single resonance, as dictated by time-reversal symmetry considerations. Our work may shed light on the design of flat subwavelength free-space nonreciprocal metasurface switches for pulsed operation which are easy to fabricate, fully passive, and require low operation power. Our simulated devices demonstrate a transmission ratio >50 dB for oppositely propagating waves, an operational bandwidth exceeding 600 GHz, and an insertion loss of <0.04 dB.
Akinetic Swept-Source Master–Slave-Enhanced Optical Coherence Tomography
Authors: Marques Manuel , Cernat Ramona , Ensher Jason , Bradu Adrian , Podoleanu Adrian
Published: April 2021

This paper presents a different approach for processing the signal from interferometers driven by swept sources that exhibit non-linear tuning during stable time intervals. Such sources are, for example, those commercialised by Insight, which are electrically tunable and akinetic. These Insight sources use a calibration procedure to skip frequencies already included in a spectral sweep, i.e., a process of “clearing the spectrum”. For the first time, the suitability of the Master–Slave (MS) procedure is evaluated as an alternative to the conventional calibration procedure for such sources. Here, the MS process is applied to the intact, raw interferogram spectrum delivered by an optical coherence tomography (OCT) system. Two modalities are investigated to implement the MS processing, based on (i) digital generation of the Master signals using the OCT interferometer and (ii) down-conversion using a second interferometer driven by the same swept source. The latter allows near-coherence-limited operation at a large axial range (>80 mm), without the need for a high sampling rate digitiser card to cope with the large frequency spectrum generated, which can exceed several GHz. In both cases, the depth information is recovered with some limitations as described in the text.
Genome-Wide Analysis and Expression Profiles of the Dof Family in Cleistogenes songorica under Temperature, Salt and ABA Treatment
Authors: Wang Penglei , Yan Zhuanzhuan , Zong Xifang , Yan Qi , Zhang Jiyu
Published: April 2021

The DNA-binding with one zinc finger (Dof) family of plant-specific transcription factors has a variety of important functions in gene transcriptional regulation, development, and stress responses. However, the structure and expression patterns of Dof family have not been identified in Cleistogenes songorica, which is an important xerophytic and perennial gramineous grass in desert grassland. In this study, 50 Dof genes were identified in C. songorica and could be classified into four groups. According to genome-wide analysis, 46 of 50 Dof genes were located on 20 chromosomes, and the gene structure and conserved protein motif of these proteins were analyzed. In addition, phylogenetic analysis of Dof genes in C. songorica, Arabidopsis thaliana, Oryza sativa, and Brachypodium distachyon estimated the evolutionary relationships, and these genes were grouped into seven clusters. Moreover, the expression profiles of these Dof genes in C. songorica were analyzed in response to high/low temperature, salinity, and ABA treatments. These results will provide valuable information for future studies on gene classification, cloning, and functional characterization of this family in C. songorica.