bluuebunny/crossref_metadata_2025 huggingface dataset:
Structural Complexity and Seismogenesis: The Role of the Transpressive Structures in the 1976 Friuli Earthquakes (Eastern Southern Alps, NE Italy)
Authors: Patricelli Giulia , Poli Maria Eliana , Cheloni Daniele
Published: May 2022

We reconstructed the seismotectonic setting of the area comprising the northeastern Friuli Plain and the Julian pre-Alpine border (NE Italy) by integrating geological and seismological data. The study area represents the junction between the SSE-verging polyphase thrust-front of the south-Alpine Chain and the NW–SE-trending strike-slip faults of the eastern Friuli–western Slovenia domain. Following a multidisciplinary approach, the 3D geometry of the Susans–Tricesimo thrust system was reconstructed through the elaboration of four geological cross sections derived from the interpretation of ENI industrial seismic lines. In a second step, the seismogenic volume of the central-eastern Friuli area was investigated through hypocentral distribution analysis: the seismic events of the latest 50 years (1976–1977 and 1978–2019 time intervals) were plotted on four NE-SW-oriented seriated sections together with the fault plane’s geometry. Through this procedure, we were able to investigate the relationship between the NW-SE-striking high-angle faults, which characterize the northern Julian pre-Alps, and the WSW-verging medium-angle reverse fronts located at the piedmont of the Friuli plain, which experienced NW-SE- to NNW-SSE-oriented compression starting at least from the Pliocene. In detail, we examined the involvement of these structures during the seismic sequences of May and September 1976, in terms of activation and/or interaction. The resulting seismotectonic model highlights the interplay between transpressive/strike-slip and reverse planes. In particular, this study suggests that Predjama and Maniaglia transpressive faults strongly control the stress release and likely played a fundamental role both during the 6 May (Mw 6.5) and 15 September (Mw 6.0) Friuli earthquakes.
GRACE Accelerometers Sensitive to Ionosphere Plasma Waves: Similarities between Twangs and Whistlers
Authors: Schlicht Anja
Published: May 2022

Electrostatic space accelerometers are high-precision instruments used in gravity and magnetic fields and in fundamental physics missions. From their first use in space, these instruments show disturbances around the read-out frequency. In recent missions like Swarm and GRACE-FO, these instruments are seriously compromised. Their capability cannot be fully exploited or, in the worst case, not used for science data processing at all. We currently neither understand the mechanism nor the cause of the disturbance. For that reason, every correlation, direct or indirect, between signatures in the accelerometer data and other phenomena in the satellite environment is very important to study. In the GRACE mission, some of the disturbances relate to onboard current switching processes, such as switching of heater and torquer currents. In this paper, we examine the phenomena called twangs. The shape of these disturbances is responsible for their name, because they look like a decaying tone. Some of these twangs seem to relate to changing currents, too. They occur when sunlight hits surfaces of the satellite. This can cause the discharging of satellite parts. Here, we focus on special twangs, which we call cloud-related twangs, as recent investigations related them to cloud coverage. How can clouds in the troposphere influence a satellite, orbiting in the ionosphere? We investigated the troposphere–ionosphere coupling and found that the only coupling which can be responsible for disturbances on satellites and which may affect the accelerometers, are electromagnetic waves traveling in the so-called whistler mode at frequencies in the very-low-frequency (VLF) domain. This special propagation mode is a coupled electromagnetic and electrostatic mode. There, the electric field vector has a component in the direction of propagation due to the interaction with ions and the magnetic field in the ionosphere and magnetosphere. This led us to investigate the similarities between whistlers and twangs. The hypothesis that they are related is very important in our further understanding of disturbances on satellite instruments sensible to electromagnetic pulses in the range of very low frequencies and below.
Students’ Mental Health, Well-Being, and Loneliness during the COVID-19 Pandemic: A Cross-National Study
Authors: Bonsaksen Tore , Chiu Vivian , Leung Janni , Schoultz Mariyana , Thygesen Hilde , Price Daicia , Ruffolo Mary , Geirdal Amy Østertun
Published: May 2022

During the COVID-19 pandemic, much research has been devoted to assessing mental health in a variety of populations. Students in higher education appear to be particularly vulnerable to experiencing reduced mental health. The purpose of the study was to assess whether higher education students experienced poorer mental health compared to the general population and examine the factors associated with students’ mental health during the COVID-19 pandemic. A cross-national sample of students (n = 354) and non-students (n = 3120) participated in a survey in October/November 2020. Mental health outcomes among students and non-students were compared with independent t-tests. Multiple linear regression analysis and general linear estimation were used to assess the impact of student status on mental health outcomes while adjusting for sociodemographic factors. Students reported poorer mental health than non-students. The difference in mental health between students and non-students was bigger for participants aged 30 years or older. More social media use was associated with poorer mental health outcomes. In conclusion, students had poorer mental health than the wider population. Aspects of life as a student, beyond what can be attributed to life stage, appears to increase mental health problems.
The Effects of Bagging on Color Change and Chemical Composition in ‘Jinyan’ Kiwifruit (Actinidia chinensis)
Authors: Xu Yunhe , Liu Yafang , Li Wanqing , Yang Caining , Lin Yujia , Wang Yuanyuan , Chen Chuying , Wan Chunpeng , Chen Jinyin , Gan Zengyu
Published: May 2022

To explore the effect of bagging on the nutritional quality and color of kiwifruit (Actinidia spp.), the fruits of yellow-fleshed kiwifruit cultivars were analyzed after bagging treatment. Bagging treatment promoted the degreening of mesocarp and increased brightness. Bagging significantly reduced the accumulation of dry matter, titratable acids, starch, sucrose, fructose, and glucose during kiwifruit development. Additionally, bagging significantly reduced the accumulation of chlorophyll and carotenoids during development, whereas after debagging, the chlorophyll and carotenoid contents were significantly increased. Gene expression analysis showed that during most of the fruit development periods, the chlorophyll biosynthesis genes AcRCBS, AcGLUTR, and AcCHLG, and degradation genes AcCBR, AcPAO, AcPPH, AcCLH, and AcSGR had significantly lower expression levels in bagged fruit. Bagging also inhibited the expression of carotenoid metabolism genes, especially AcSGR and AcLCYB, which may play a key role in the process of fruit development during bagging by decreasing the accumulation of chlorophyll and carotenoids in kiwifruit. Additionally, bagging significantly reduced the content of AsA. The expression of the AsA biosynthesis genes AcPMI2, AcGPP2, and AcGalDH in bagged fruit was significantly lower than in the control, indicating that these may be the key genes responsible for the difference in the accumulation of AsA after bagging.
An Unethical Organizational Behavior for the Sake of the Family: Perceived Risk of Job Insecurity, Family Motivation and Financial Pressures
Authors: Elshaer Ibrahim A. , Ghanem Marwa , Azazz Alaa M. S.
Published: May 2022

In organizations, unethical behaviors are pervasive and costly, and considerable recent research attention has been paid to various types of workplace unethical behavior. This study examines employees’ behaviors that are carried out for the benefit of one’s family but violate societal and organizational moral standards. Drawing upon the self-maintenance and bounded ethicality theories, this study examines the engagement of unethical organization behaviors (UOB) in the name of the family during the COVID-19 pandemic. It examines the influence of job instability and the mediating role of family financial pressure and family motivation. A total of 770 employees in hotels and travel agents in Egypt were targeted, and the data were analyzed using structural equation modeling. The results posit that perceived risk of job insecurity predicts engagement in unethical organizational behaviors, while intentions of UOB increase by high family motivation and financial pressures. Toward the end of this paper, a discussion on the theoretical and practical implications and are presented.
Development of Core Educational Content for Heart Failure Patients in Transition from Hospital to Home Care: A Delphi Study
Authors: Lee Seo-Jin , Kim Bo-Hwan
Published: May 2022

Heart failure (HF) patients should be systematically educated before discharge on how to manage with standard written materials for patient self-management. However, because of the absence of readily available written materials to reinforce their learned knowledge, patients with HF feel inadequately informed in terms of the discharge information provided to them. This study aimed to develop core content to prepare patients with HF for transition from hospital to home care. The content was validated by expert panelists using Delphi methods. Nineteen draft items based on literature review were developed. We established a consensus on four core sections, including 47 categories and 128 subcategories through the Delphi survey: (1) understanding HF (five categories and 23 subcategories), (2) HF medication (19 categories and 45 subcategories), (3) HF management (20 categories and 47 subcategories), and (4) HF diary (three categories and 13 subcategories). Each section provided easy-to-understand educational contents using cartoon images and large or bold letters for older patients with HF. The developed core HF educational contents showed high consensus between the experts, along with clinical validity. The contents can be used as an educational booklet for both planning discharge education of patients with HF and for post-discharge management when transitioning from hospital to home. Based on this study, a booklet series for HF patients was first registered at the National Library of Korea. Future research should focus on delivering the core content to patients with HF in convenient and accessible format through various media.
Increased Risk of COVID-19 in Patients with Diabetes Mellitus—Current Challenges in Pathophysiology, Treatment and Prevention
Authors: Gęca Tomasz , Wojtowicz Kamila , Guzik Paweł , Góra Tomasz
Published: May 2022

Coronavirus disease—COVID-19 (coronavirus disease 2019) has become the cause of the global pandemic in the last three years. Its etiological factor is SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus type 2). Patients with diabetes (DM—diabetes mellitus), in contrast to healthy people not suffering from chronic diseases, are characterised by higher morbidity and mortality due to COVID-19. Patients who test positive for SARCoV-2 are at higher risk of developing hyperglycaemia. In this paper, we present, analyse and summarize the data on possible mechanisms underlying the increased susceptibility and mortality of patients with diabetes mellitus in the case of SARS-CoV-2 infection. However, further research is required to determine the optimal therapeutic management of patients with diabetes and COVID-19.
Changes in Attitudes toward Tobacco Smoking and Factors Associated with Quitting in 9-Year Observation of PURE Poland Cohort Study
Authors: Połtyn-Zaradna Katarzyna , Psikus Piotr , Zatońska Katarzyna
Published: May 2022

(1) Background: This study aims to examine changes in tobacco smoking prevalence in the PURE Poland cohort study over the 9-year follow-up period. Moreover, it attempts to identify socio-demographic factors that affect changes in attitudes towards tobacco smoking. (2) Methods: The PURE Poland cohort study—baseline was performed in 2007–2010 and covered 2036 participants, including urban (59.4%) and rural (40.6%) residents of Lower Silesia, Poland. The following study reports the results of 1690 participants who took part in both the baseline (2007–2010) study and 9-year follow-up (2016–2019). (3) Results: There was a 3.5% decrease in current smokers during the analyzed period (from 20.2% at the baseline study to 16.7% in the 9-year follow-up). Living in rural area increased the likelihood of being a current smoker by more than 1.5-fold (OR = 1.65 CI = 1.26–2.14) and decreased the likelihood of being a former smoker (OR = 0.70 CI = 0.57–0.86). In the 9-year follow-up period, more women were current smokers than men (17.2% vs. 16.0%) and women had lower chances of being former smokers than men (OR = 0.77 CI = 0.62–0.95). People with a primary education had 1.5-fold higher likelihood of being a current smoker (OR = 1.45 CI = 1.03–2.05). Nearly 11% significant increase in the percentage of current smokers was observed in the oldest age group (1.9% in the baseline study vs. 12.6% in the follow-up period). (4) Conclusions: The results obtained during 9 years of observation indicate the necessity of intensifying anti-tobacco programs especially targeting women, elderly population, people with lower level of education, rural residents, and the unemployed.
Factors Associated with Treatment Outcomes and Pathological Features in Patients with Osteoradionecrosis: A Retrospective Study
Authors: Tadokoro Yoshiaki , Hasegawa Takumi , Takeda Daisuke , Murakami Aki , Yatagai Nanae , Iwata Eiji , Saito Izumi , Kusumoto Junya , Akashi Masaya
Published: May 2022

A standard treatment for osteoradionecrosis (ORN) has not yet been established because of the diversity. Therefore, identifying the risk factors for a poor prognosis is essential. This study retrospectively investigated the factors associated with the prognosis of ORN in 68 patients. Relevant clinical data of all patients were obtained. Of the patients, 16 who underwent extensive surgery underwent histopathological analysis. The necrotic changes of the anterior and posterior margins in the cortical and cancellous bones were investigated. Multivariate analyses showed statistically significant associations between poor prognosis in patients with ORN and high radiation dose (hazard ratio [HR] 1.15), orocutaneous fistula (HR 2.93), and absence of sequestration (HR 2.49). Histopathological analysis showed a viable anterior margin of the middle portion of the cortical bone for all recovered cases; in contrast, most cases (75%) with a poor prognosis showed necrotic changes. The anterior margin of the cancellous bone was viable and resilient to high irradiation, regardless of the prognosis. These results suggest that patients with orocutaneous fistula should receive early surgical intervention, even if the affected area is limited or asymptomatic. In extensive surgery, a sufficient safety margin of necrotic bone, particularly in the anterior region, is required to improve the prognosis.
School Surrounding Region Traffic Commuting Analysis Based on Simulation
Authors: Liu Huasheng , Deng Haoran , Li Yu , Zhao Yuqi , Li Xiaowen
Published: May 2022

Student commuting is an important part of urban travel demand and private car commuting plays an important role in urban traffic, especially in areas near schools. Since parents, especially the parents of elementary and junior high school students, prefer to drive rather than take public transport, there will be a negative effect on traffic management. To address the challenge, a simulation model is established based on schools’ surrounding regions to analyze traffic status. Specifically, the model focuses on urban construction and transportation near the entrance of schools and neighborhoods. In addition, four variable parameters consisting of the directional hourly volume, the parking demand of delivery vehicles, the distance between the school and intersection, and the average parking time for pick-up vehicles are set as influence factors, while traffic efficiency, energy consumption, and pollutant emissions are considered as the evaluation criteria of our model. Extensive simulated experiments show that comparing different scenarios, the traffic state of schools’ surrounding areas can achieve much better performance when the distance between entrances and intersections is 400 m under the 1000 pcu/h condition. This research can provide a scientific basis for school regional traffic management and organization optimization.
Application of Multi-Criteria Decision-Making Analysis to Rural Spatial Sustainability Evaluation: A Systematic Review
Authors: Yuan Zheng , Wen Baohua , He Cheng , Zhou Jin , Zhou Zhonghua , Xu Feng
Published: May 2022

The rational allocation of spatial resources is an important factor to ensure the sustainable development of rural areas, and effective pre-emptive spatial evaluation is the prerequisite for identifying the predicament of rural resource allocation. Multi-criteria decision-making analysis has advantages in solving multi-attribute and multi-objective decision-making problems, and has been used in sustainability evaluation research in various disciplines in recent years. Previous studies have proved the value of spatial evaluation using multi-criteria decision analysis in guiding rural incremental development and inventory updates, but systematic reviews of the previous literature from a multidisciplinary perspective and studies of the implementation steps of the evaluation framework are lacking. In the current paper, the research is reviewed from the two levels of quantitative statistics and research content, and through vertical and horizontal comparisons based on three common operating procedures: standard formulation, weight distribution, and ranking and verification. Through the results, the application status and characteristics of the MCDA method in related research are determined, and five research foci in the future are proposed.
Searching for the Molecular Basis of Partial Deafness
Authors: Oziębło Dominika , Bałdyga Natalia , Leja Marcin L. , Skarżyński Henryk , Ołdak Monika
Published: May 2022

Hearing is an important human sense for communicating and connecting with others. Partial deafness (PD) is a common hearing problem, in which there is a down-sloping audiogram. In this study, we apply a practical system for classifying PD patients, used for treatment purposes, to distinguish two groups of patients: one with almost normal hearing thresholds at low frequencies (PDT-EC, n = 20), and a second group with poorer thresholds at those same low frequencies (PDT-EAS, n = 20). After performing comprehensive genetic testing with a panel of 237 genes, we found that genetic factors can explain a significant proportion of both PDT-EC and PDT-EAS hearing losses, accounting, respectively, for approx. one-fifth and one-half of all the cases in our cohort. Most of the causative variants were located in dominant and recessive genes previously linked to PD, but more than half of the variants were novel. Among the contributors to PDT-EC we identified OSBPL2 and SYNE4, two relatively new hereditary hearing loss genes with a low publication profile. Our study revealed that, for all PD patients, a postlingual hearing loss more severe in the low-frequency range is associated with a higher detection rate of causative variants. Isolating a genetic cause of PD is important in terms of prognosis, therapeutic effectiveness, and risk of recurrence.
Expression of a Chlorophyll b Reductase Gene from Zoysia japonica Causes Changes in Leaf Color and Chlorophyll Morphology in Agrostis stolonifera
Authors: Dong Di , Yang Zhuoxiong , Ma Yuan , Li Shuwen , Wang Mengdi , Li Yinruizhi , Liu Zhuocheng , Han Liebao , Chao Yuehui
Published: May 2022

The NYC-like (NOL) enzyme is considered as an essential enzyme for chlorophyll b degradation, which catalyzes the formation of 7-hydroxymethyl chlorophyll a from chlorophyll b. The ZjNOL gene was cloned from Zoysia japonica with a completed coding sequence of 981-bp in length, encoding 326 amino acids. ZjNOL was localized on the stroma side of the thylakoid membrane, and co-localized with ZjNYC in the chloroplasts. Multiple photoregulatory elements and hormone regulatory elements were identified in the promoter region of the ZjNOL gene, and the expression level of the ZjNOL gene was dramatically up-regulated in senescence leaves, which were regulated by a variety of plant hormones. ZjNOL’s ectopic expression in creeping bentgrass produced yellow leaves, thicker cortex, and smaller vascular column cells. Additionally, transgenic plants exhibited morphological alterations in their chloroplast structure, and the number of grana and thylakoids per grana stack reduced dramatically. Transgenic plants also had a lower photosynthetic rate and Fm/Fv than the control. The transgenic plants displayed a decreased chlorophyll content and a greater rate of ion leakage. The properties and activities of ZjNOL will serve as a foundation for future research into gene functions and regulatory processes.
The Strong Enhancement of Electron-Impact Ionization Processes in Dense Plasma by Transient Spatial Localization
Authors: Zeng Jiaolong , Ye Chen , Liu Pengfei , Gao Cheng , Li Yongjun , Yuan Jianmin
Published: May 2022

Recent experiments have observed much higher electron–ion collisional ionization cross sections and rates in dense plasmas than predicted by the current standard atomic collision theory, including the plasma screening effect. We suggest that the use of (distorted) plane waves for incident and scattered electrons is not adequate to describe the dissipation that occurs during the ionization event. Random collisions with free electrons and ions in plasma cause electron matter waves to lose their phase, which results in the partial decoherence of incident and scattered electrons. Such a plasma-induced transient spatial localization of the continuum electron states significantly modifies the wave functions of continuum electrons, resulting in a strong enhancement of the electron–ion collisional ionization of ions in plasma compared to isolated ions. Here, we develop a theoretical formulation to calculate the differential and integral cross sections by incorporating the effects of plasma screening and transient spatial localization. The approach is then used to investigate the electron-impact ionization of ions in solid-density magnesium plasma, yielding results that are consistent with experiments. In dense plasma, the correlation of continuum electron energies is modified, and the integral cross sections and rates increase considerably. For the ionization of Mg9+e+1s22s2S→1s21S+2e, the ionization cross sections increase several-fold, and the rates increase by one order of magnitude. Our findings provide new insight into collisional ionization and three-body recombination and may aid investigations of the transport properties and nonequilibrium evolution of dense plasma.
Tissue-Based Markers as a Tool to Assess Response to Neoadjuvant Radiotherapy in Rectal Cancer—Systematic Review
Authors: Smolskas Edgaras , Mikulskytė Goda , Sileika Ernestas , Suziedelis Kestutis , Dulskas Audrius
Published: May 2022

According to current guidelines, the current treatment for locally advanced rectal cancer is neoadjuvant therapy, followed by a total mesorectal excision. However, radiosensitivity tends to differ among patients due to tumor heterogeneity, making it difficult to predict the possible outcomes of the neoadjuvant therapy. This review aims to investigate different types of tissue-based biomarkers and their capability of predicting tumor response to neoadjuvant therapy in patients with locally advanced rectal cancer. We identified 169 abstracts in NCBI PubMed, selected 48 reports considered to meet inclusion criteria and performed this systematic review. Multiple classes of molecular biomarkers, such as proteins, DNA, micro-RNA or tumor immune microenvironment, were studied as potential predictors for rectal cancer response; nonetheless, no literature to date has provided enough sufficient evidence for any of them to be introduced into clinical practice.
Expression Profiles of Circulating MicroRNAs in XELOX-Chemotherapy-Induced Peripheral Neuropathy in Patients with Advanced Gastric Cancer
Authors: Ju Yeongdon , Seol Young Mi , Kim Jungho , Jin Hyunwoo , Choi Go-Eun , Jang Aelee
Published: May 2022

Gastric cancer (GC) is one of the most common cancers and a leading cause of cancer deaths around the world. Chemotherapy is one of the most effective treatments for cancer patients, and has remarkably enhanced survival rates. However, it has many side effects. Recently, microRNAs (miRNAs) have been intensively studied as potential biomarkers for cancer diagnosis and treatment monitoring. However, definitive biomarkers in chemotherapy-induced peripheral neuropathy (CIPN) are still lacking. The aim of this study was to identify the factors significant for neurological adverse events in GC patients receiving XELOX (oxaliplatin and capecitabine) chemotherapy. The results show that XELOX chemotherapy induces changes in the expression of hsa-miR-200c-3p, hsa-miR-885-5p, and hsa-miR-378f. Validation by qRT-PCR demonstrated that hsa-miR-378f was significantly downregulated in CIPN. Hsa-miR-378f was identified as showing a statistically significant correlation in GC patients receiving XELOX chemotherapy according to the analysis of differentially expressed (DE) miRNAs. Furthermore, 34 potential target genes were predicted using a web-based database for miRNA target prognostication and functional annotations. The identified genes are related to the peptidyl-serine phosphorylation and regulation of alternative mRNA splicing with enrichment in the gastric cancer, neurotrophin, MAPK, and AMPK signaling pathways. Collectively, these results provide information useful for developing promising strategies for the treatment of XELOX-chemotherapy-induced peripheral neuropathy.
Development of Nd (III)-Based Terahertz Absorbers Revealing Temperature Dependent Near-Infrared Luminescence
Authors: Kumar Kunal , Stefanczyk Olaf , Nakabayashi Koji , Mineo Yuuki , Ohkoshi Shin-ichi
Published: May 2022

Molecular vibrations in the solid-state, detectable in the terahertz (THz) region, are the subject of research to further develop THz technologies. To observe such vibrations in terahertz time-domain spectroscopy (THz-TDS) and low-frequency (LF) Raman spectroscopy, two supramolecular assemblies with the formula [NdIII (phen)3 (NCX)3] 0.3EtOH (X = S, 1-S; Se, 1-Se) were designed and prepared. Both compounds show several THz-TDS and LF-Raman peaks in the sub-THz range, with the lowest frequencies of 0.65 and 0.59 THz for 1-S and 1-Se, and 0.75 and 0.61 THz for 1-S and 1-Se, respectively. The peak redshift was observed due to the substitution of SCN− by SeCN−. Additionally, temperature-dependent TDS-THz studies showed a thermal blueshift phenomenon, as the peak position shifted to 0.68 THz for 1-S and 0.62 THz for 1-Se at 10 K. Based on ab initio calculations, sub-THz vibrations were ascribed to the swaying of the three thiocyanate/selenocyanate. Moreover, both samples exhibited near-infrared (NIR) emission from Nd (III), and very good thermometric properties in the 300–150 K range, comparable to neodymium (III) oxide-based thermometers and higher than previously reported complexes. Moreover, the temperature dependence of fluorescence and THz spectroscopy analysis showed that the reduction in anharmonic thermal vibrations leads to a significant increase in the intensity and a reduction in the width of the emission and LF absorption peaks. These studies provide the basis for developing new routes to adjust the LF vibrational absorption.
How Do Phenolic Acids Change the Secondary and Tertiary Structure of Gliadin? Studies with an Application of Spectroscopic Techniques
Authors: Welc Renata , Luchowski Rafał , Kłosok Konrad , Gruszecki Wiesław I. , Nawrocka Agnieszka
Published: May 2022

The effect of the chemical structure of selected phenolic acids on the molecular organization of gliadins was investigated with the application of Fourier Transform Infrared (FTIR) technique, steady-state, and time-resolved fluorescence spectroscopy. Hydroxybenzoic (4-hydroxybenzoic, protocatechuic, vanillic, and syringic) and hydroxycinnamic (coumaric, caffeic, ferulic, sinapic) acids have been used as gliadins modifiers. The results indicated that hydroxybenzoic acids due to their smaller size incorporate into spaces between two polypeptide chains and form a hydrogen bond with them leading to aggregation. Additionally, syringic acids could incorporate into hydrophobic pockets of protein. Whereas hydroxycinnamic acids, due to their higher stiffness and larger size, separated polypeptide chains leading to gliadin disaggregation. These acids did not incorporate into hydrophobic pockets.
Alteration of Gene and miRNA Expression in Cervical Intraepithelial Neoplasia and Cervical Cancer
Authors: Dudea-Simon Marina , Mihu Dan , Pop Laura Ancuta , Ciortea Razvan , Malutan Andrei Mihai , Diculescu Doru , Ciocan Cristina Alexandra , Cojocneanu Roxana Maria , Simon Vasile , Bucuri Carmen , Mocan-Hognogi Radu , Braicu Cornelia , Berindan-Neagoe Ioana
Published: May 2022

Background: Cervical cancer is one of the most common malignancies in women in terms of prevalence and mortality. Cervical cancer has some particularities that distinguish it from any other oncologic pathology: first, it is completely preventable by prompt detection of its precursor, cervical intraepithelial neoplasia (CIN); second, the Human Papillomavirus (HPV) infection is a known etiological agent; third, the mean age at diagnosis is much lower than in other oncologic conditions, as a consequence of the sexually-transmitted HPV. Methods: We evaluated the expression level of several long noncoding RNAs and a microRNA in samples from 30 patients with CIN, 9 with cervical cancer and 38 normal samples using qRT-PCR technology. Results: We observed higher expression levels for MEG3, DAPK1, MLH1 and MALAT1 in CIN samples than in normal samples, whereas TIMP3 and SOX1 had lower expression levels. For cancer samples, DAPK1, MLH1 and MALAT1 had higher expression, and MEG3, TIMP3 and SOX1 had lower expression when compared to normal samples. In the case of CIN versus cancer samples, only MEG3 gene showed a statistically significant difference. The expression of miR-205-5p was lower in both CIN and cancer samples compared to normal samples. Conclusion: Decreased MEG3 expression could be considered an alarm signal in the transition from a premalignant cervical lesion to invasive cancer, while altered expression levels of TIMP3, SOX1, MLH1, MALAT1 and miR-205-5p could serve as early biomarkers in the diagnosis of premalignant cervical lesions. Future studies, including a larger number of patients with CIN, will be of particular importance in validating these observations.
Synuclein Analysis in Adult Xenopus laevis
Authors: Bonaccorsi di Patti Maria Carmela , Angiulli Elisa , Casini Arianna , Vaccaro Rosa , Cioni Carla , Toni Mattia
Published: May 2022

The α-, β- and γ-synucleins are small soluble proteins expressed in the nervous system of mammals and evolutionary conserved in vertebrates. After being discovered in the cartilaginous fish Torpedo californica, synucleins have been sequenced in all vertebrates, showing differences in the number of genes and splicing isoforms in different taxa. Although α-, β- and γ-synucleins share high homology in the N-terminal sequence, suggesting their evolution from a common ancestor, the three isoforms also differ in molecular characteristics, expression levels and tissue distribution. Moreover, their functions have yet to be fully understood. Great scientific interest on synucleins mainly derives from the involvement of α-synuclein in human neurodegenerative diseases, collectively named synucleinopathies, which involve the accumulation of amyloidogenic α-synuclein inclusions in neurons and glia cells. Studies on synucleinopathies can take advantage of the development of new vertebrate models other than mammals. Moreover, synuclein expression in non-mammalian vertebrates contribute to clarify the physiological role of these proteins in the evolutionary perspective. In this paper, gene expression levels of α-, β- and γ-synucleins have been analysed in the main organs of adult Xenopus laevis by qRT-PCR. Moreover, recombinant α-, β- and γ-synucleins were produced to test the specificity of commercial antibodies against α-synuclein used in Western blot and immunohistochemistry. Finally, the secondary structure of Xenopus synucleins was evaluated by circular dichroism analysis. Results indicate Xenopus as a good model for studying synucleinopathies, and provide a useful background for future studies on synuclein functions and their evolution in vertebrates.
Biochemical, Structural Analysis, and Docking Studies of Spiropyrazoline Derivatives
Authors: Adamus-Grabicka Angelika A. , Daśko Mateusz , Hikisz Pawel , Kusz Joachim , Malecka Magdalena , Budzisz Elzbieta
Published: May 2022

In this study, we evaluated the antiproliferative potential, DNA damage, crystal structures, and docking calculation of two spiropyrazoline derivatives. The main focus of the research was to evaluate the antiproliferative potential of synthesized compounds towards eight cancer cell lines. Compound I demonstrated promising antiproliferative properties, especially toward the HL60 cell line, for which IC50 was equal to 9.4 µM/L. The analysis of DNA damage by the comet assay showed that compound II caused DNA damage to tumor lineage cells to a greater extent than compound I. The level of damage to tumor cells of the HEC-1-A lineage was 23%. The determination of apoptotic and necrotic cell fractions by fluorescence microscopy indicated that cells treated with spiropyrazoline-based analogues were entering the early phase of programmed cell death. Compounds I and II depolarized the mitochondrial membranes of cancer cells. Furthermore, we performed simple docking calculations, which indicated that the obtained compounds are able to bind to the PARP1 active site, at least theoretically (the free energy of binding values for compound I and II were −9.7 and 8.7 kcal mol−1, respectively). In silico studies of the influence of the studied compounds on PARP1 were confirmed in vitro with the use of eight cancer cell lines. The degradation of the PARP1 enzyme was observed, with compound I characterized by a higher protein degradation activity.
Working Conditions and Work Engagement by Gender and Digital Work Intensity
Authors: Rodríguez-Modroño Paula
Published: May 2022

Telework and other flexible working arrangements, which have exponentially expanded with new advancements in digitalization and the impact of COVID-19, are modifying working conditions and workers’ engagement. Using the ‘job demands-resources’ model, we applied multivariate techniques to examine the different ways in which telework intensity impacts working conditions by gender. Increased intensity of remote working was positively associated with better skills and discretion and work engagement, while it was negatively associated with the other dimensions of job quality (particularly with working time quality). Even though women usually score higher than men in work intensity or working time quality, high intense female teleworkers experience a downturn with respect to these two items. Low and medium intensities of teleworking were positively associated with skills and discretion, working time quality, improved physical environment, and especially with better prospects and earnings. In conclusion, the intensity of teleworking and gender affect job quality and work engagement in different degrees, highlighting the importance of including these multiple effects on the design of flexible working arrangements.
Kinetic Control of Anion Stoichiometry in Hexagonal BaTiO3
Authors: Kageyama Keisuke , Yang Yang , Kageyama Toki , Murayama Kantaro , Shitara Kazuki , Saito Takashi , Ubukata Hiroki , Tassel Cédric , Kuwabara Akihide , Kageyama Hiroshi
Published: May 2022

The cubic oxyhydride perovskite BaTiO3−xHx, where the well-known ferroelectric oxide BaTiO3 is partially hydridized, exhibits a variety of functions such as being a catalyst and precursor for the synthesis of mixed-anion compounds by utilizing the labile nature of hydride anions. In this study, we present a hexagonal version, BaTi(O3−xHx) (x < 0.6) with the 6H-type structure, synthesized by a topochemical reaction using hydride reduction, unlike reported hexagonal oxyhydrides obtained under high pressure. The conversion of cubic BaTiO3 (150 nm) to the hexagonal phase by heat treatment at low temperature (950~1025 °C) using a Mg getter allows the introduction of large oxygen defects (BaTiO3−x; x − 0.28) while preventing the crystal growth of hexagonal BaTiO3, which has been accessible at high temperatures of ~1500 °C, contributing to the increase of the hydrogen content. Hydride anions in 6H-BaTiO3−xHx preferentially occupy face-sharing sites, as do other oxyhydrides.
Effects of Juvenile Hormone Analog and Days after Emergence on the Reproduction of Oriental Armyworm, Mythimna separata (Lepidoptera: Noctuidae) Populations
Authors: Lv Weixiang , Zeng Liting , Zhang Zhe , He Hengguo , Wang Fang , Xie Xingcheng
Published: May 2022

Mythimna separata (Walker) is a main cereal crop pest that causes extensive damage to the world grain production. The effects of juvenile hormone on M. separata populations remain poorly understood. Here, we explored how JH analog (JHA) affected reproductive traits of both migrant and resident populations in this pest. Our results showed that the influence of JHA treatment on reproduction depended on adult age (days after emergence) of exposure to JHA and populations. Exposure of M. separata residents to JHA (methoprene) on day 1 and day 2 after adult emergence significantly shortened the pre-oviposition period, but increased the lifetime fecundity, mating frequency and grade of ovarian development compared to the controls. However, M. separata migrants exposed to JHA only on day 1 facilitated their reproduction, resulting in a reduction in the pre-oviposition period but an increase in lifetime fecundity, mating frequency and grade of ovarian development. In addition, exposure to JHA from day 2 to day 4 did not significantly affect the ovarian and reproductive development in both migrant and resident populations. These results indicated that the first two days after adult emergence were the sensitive period for residents. In contrast, only the first day after adult emergence was the sensitive stage for migrants. Our findings will contribute to a better understanding of JHA function on M. separata populations.
Responses of Six Wheat Cultivars (Triticum aestivum) to Wheat Aphid (Sitobion avenae) Infestation
Authors: Zhang Ke-Xin , Li Hong-Yan , Quandahor Peter , Gou Yu-Ping , Li Chun-Chun , Zhang Qiang-Yan , Haq Inzamam Ul , Ma Yue , Liu Chang-Zhong
Published: May 2022

Resistant variety screening is widely recommended for the management of Sitobion avenae. The purpose of this study was to assess responses of six wheat varieties (lines) to S. avenae. The aphid quantity ratio (AQR) was used to assess S. avenae resistance. Pearson’s correlation coefficient was used to perform a correlation analysis between AQR, biological parameters, and the accumulation of total phenolic and flavonoid content. When compared to the other cultivars, the results showed that two cultivars, Yongliang No.15 and Ganchun No.18, had high resistance against S. avenae. The correlation analysis revealed a positive relationship between total phenol and flavonoid content accumulation and developmental duration (DD), and a negative relationship between accumulation and weight gain (WG) and mean relative growth rate (MRGR). The correlation between flavonoid and biological parameters was statistically stronger than the correlation between total phenol and biological parameters. This research provides critical cues for screening and improving aphid-resistant wheat varieties in the field and will aid in our understanding of the resistance mechanism of wheat varieties against S. avenae.
A Fingerprint Matching Algorithm Using the Combination of Edge Features and Convolution Neural Networks
Authors: Dincă Lăzărescu Andreea-Monica , Moldovanu Simona , Moraru Luminita
Published: May 2022

This study presents an algorithm for fingerprint classification using a CNN (convolutional neural network) model and making use of full images belonging to four digital databases. The main challenge that we face in fingerprint classification is dealing with the low quality of fingerprints, which can impede the identification process. To overcome these restrictions, the proposed model consists of the following steps: a preprocessing stage which deals with edge enhancement operations, data resizing, data augmentation, and finally a post-processing stage devoted to classification tasks. Primarily, the fingerprint images are enhanced using Prewitt and Laplacian of Gaussian filters. This investigation used the fingerprint verification competition with four databases (FVC2004, DB1, DB2, DB3, and DB4) which contain 240 real fingerprint images and 80 synthetic fingerprint images. The real images were collected using various sensors. The innovation of the model is in the manner in which the number of epochs is selected, which improves the performance of the classification. The number of epochs is defined as a hyper-parameter which can influence the performance of the deep learning model. The number of epochs was set to 10, 20, 30, and 50 in order to keep the training time at an acceptable value of 1.8 s/epoch, on average. Our results indicate the overfitting of the model starting with the seventh epoch. The accuracy varies from 67.6% to 98.7% for the validation set, and between 70.2% and 75.6% for the test set. The proposed method achieved a very good performance compared to the traditional hand-crafted features despite the fact that it used raw data and it does not perform any handcrafted feature extraction operations.
The Role of ABO Blood Type in Patients with SARS-CoV-2 Infection: A Systematic Review
Authors: Banchelli Federico , Negro Pierpaolo , Guido Marcello , D’Amico Roberto , Fittipaldo Veronica Andrea , Grima Pierfrancesco , Zizza Antonella
Published: May 2022

The SARS-CoV-2 infection has caused over 422 million contagions and 5.8 million deaths resulting in a global health crisis. Several studies have investigated the risk factors predisposing to the infection and reported that the host susceptibility can be linked to the ABO blood group, but the current evidence is controversial. We systematically searched for articles in EMBASE, PubMed, and Cochrane library published up to 7 May 2021 to explore the association of the ABO blood group with the susceptibility to SARS-CoV-2 infection. All studies in people undergoing SARS-CoV-2 test controls were included. Odds ratios were obtained in each study and then synthesised by using meta-analysis. Overall, 22 articles were selected and more than 1,200,000 individuals of whom 74,563 resulted positive to SARS-CoV-2 and 1,166,717 resulted negative, were included in the meta-analysis. Overall, 487,985 subjects had blood group A, 151,879 had group B, 52,621 had group AB, and 548,795 had group O. Group O was slightly less associated with infection, as compared to the other three blood groups (OR = 0.91, 95% CI = 0.85–0.99, p = 0.02). Conversely, group A was slightly more associated with infection, as compared to the other three groups (OR = 1.06, 95% CI = 1.00–1.13, p = 0.04). This meta-analysis shows associations between blood groups and SARS-CoV-2 infection and supports the hypothesis that blood type O may have a slightly lower risk of infection, whereas blood type A may have a slightly higher risk of infection.
The Impact of Myosteatosis Percentage on Short-Term Mortality in Patients with Septic Shock
Authors: Kim June-sung , Ha Jiyeon , Kim Youn-Jung , Ko Yousun , Park Taeyong , Kim Kyung Won , Kim Won Young
Published: May 2022

The impact of myosteatosis on septic patients has not been fully revealed. The aim of the study was to evaluate the impact of the myosteatosis area and percentage on the 28-day mortality in patients with septic shock. We conducted a single center, retrospective study from a prospectively collected registry of adult patients with septic shock who presented to the emergency department and performed abdominal computed tomography (CT) from May 2016 to May 2020. The myosteatosis area defined as the sum of low attenuation muscle area and intramuscular adipose tissue at the level of the third lumbar vertebra was measured by CT. Myosteatosis percentages were calculated by dividing the myosteatosis area by the total abdominal muscle area. Of the 896 patients, 28-day mortality was 16.3%, and the abnormal myosteatosis area was commonly detected (81.7%). Among variables of body compositions, non-survivors had relatively lower normal attenuation muscle area, higher low attenuation muscle area, and higher myosteatosis area and percentage than that of survivors. Trends of myosteatosis according to age group were different between the male and female groups. In subgroup analysis with male patients, the multivariate model showed that the myosteatosis percentage (adjusted OR 1.02 [95% CI 1.01–1.03]) was an independent risk factor for 28-day mortality. However, this association was not evident in the female group. Myosteatosis was common and high myosteatosis percentage was associated with short-term mortality in patients with septic shock. Our results implied that abnormal fatty disposition in muscle could impact on increased mortality, and this effect was more prominent in male patients.
Simultaneous Quantification of Anisotropic Microcirculation and Microstructure in Peripheral Nerve
Authors: Merchant Samer , Yeoh Stewart , Mahan Mark A. , Hsu Edward W.
Published: May 2022

Peripheral nerve injury is a significant public health challenge, and perfusion in the nerve is a potential biomarker for assessing the injury severity and prognostic outlook. Here, we applied a novel formalism that combined intravoxel incoherent motion (IVIM) and diffusion tensor imaging (DTI) to simultaneously characterize anisotropic microcirculation and microstructure in the rat sciatic nerve. Comparison to postmortem measurements revealed that the in vivo IVIM-DTI signal contained a fast compartment (2.32 ± 0.04 × 10−3 mm2/s mean diffusivity, mean ± sem, n = 6, paired t test p < 0.01) that could be attributed to microcirculation in addition to a slower compartment that had similar mean diffusivity as the postmortem nerve (1.04 ± 0.01 vs. 0.96 ± 0.05 × 10−3 mm2/s, p > 0.05). Although further investigation and technical improvement are warranted, this preliminary study demonstrates both the feasibility and potential for applying the IVIM-DTI methodology to peripheral nerves for quantifying perfusion in the presence of anisotropic tissue microstructure.