bluuebunny/crossref_metadata_2025 huggingface dataset:
The Dissociation Mechanism of Poly‐α‐methylstyrene (PAMS) Dimers Induced by Spin Polarization
Authors: Yu Tianrong , Wang Jia , Jiang Wanrun , Zhang Zhanwen , Zhu Yu , Tang Yongjian , Wang Zhigang
Published: March 2018

To understand the last step of degradation mechanical of the poly‐α‐methylstyrene (PAMS), we studied the fracture mechanism of PAMS dimers. The calculations show that the PAMS dimer has two different spin‐polarized effects dissociation reaction paths. Interestingly, one path is ferromagnetic coupling triplet state, which needed to overcome the energy barrier is about 0.80 eV and corresponding to an endothermic reaction. While, another path is the antiferromagnetic coupling spin‐polarized singlet state, which needed to overcome the energy barrier is only about 0.03 eV with an exothermic reaction. Both the electron density difference and the energy decomposition show that the interaction between the two monomers is relatively strong in the transition state (TS) of singlet. This is because, in the TS of singlet state, the distance between the two monomers is closer. Our results highlight that the last dissociation of PAMS and another polymer may be through the antiferromagnetic path and which able to complete spontaneously and quickly. This finding removes a possibility of mass spectrometry experiments that origin of degradation of dimer products, and is helpful to understand the degradation process for materials.
The Effect of Heat Treatment and Precipitation on Grain Growth of TRIP Steel
Authors: Li Zhen‐Lei , Chen Dong , Kang Jian , Yuan Guo , Wang Guo‐Dong
Published: March 2018

The grain size is an important factor that determines the mechanical properties of materials. Both the temperature and time of heat treatment, and the precipitation particles have significant influence on the grain size. An investigation is conducted on two alloys, with or without vanadium addition, under different heat treatment schedules. The grain size is observed and calculated by optical microscope and computer‐based image analyzer. The precipitation is analyzed using TEM. The influence of temperature and time is greater in alloy A than B, and precipitation is more effective for the grain refinement.
Clinical glycomics for the diagnosis of congenital disorders of glycosylation
Authors: Abu Bakar Nurulamin , Lefeber Dirk J. , van Scherpenzeel Monique
Published: March 2018

Clinical glycomics comprises a spectrum of different analytical methodologies to analyze glycan structures, which provides insights into the mechanisms of glycosylation. Within clinical diagnostics, glycomics serves as a functional readout of genetic variants, and can form a basis for therapy development, as was described for PGM1‐CDG. Integration of glycomics with genomics has resulted in the elucidation of previously unknown disorders of glycosylation, namely CCDC115‐CDG, TMEM199‐CDG, ATP6AP1‐CDG, MAN1B1‐CDG, and PGM1‐CDG. This review provides an introduction into protein glycosylation and presents the different glycomics methodologies ranging from gel electrophoresis to mass spectrometry (MS) and from free glycans to intact glycoproteins. The role of glycomics in the diagnosis of congenital disorders of glycosylation (CDG) is presented, including a diagnostic flow chart and an overview of glycomics data of known CDG subtypes. The review ends with some future perspectives, showing upcoming technologies as system wide mapping of the N‐ and O‐glycoproteome, intact glycoprotein profiling and analysis of sugar metabolism. These new advances will provide additional insights and opportunities to develop personalized therapy. This is especially true for inborn errors of metabolism, which are amenable to causal therapy, because interventions through supplementation therapy can directly target the pathogenesis at the molecular level.
Principles for central nervous system inflammation research: A call for a consortium approach
Authors: Rogers Joseph
Published: March 2018

Introduction

Neuroinflammation is now considered to be of pathophysiologic significance in Alzheimer's disease and other neurodegenerative conditions. Nonetheless, there is no consensus as to what inflammatory mechanisms need to be combated (or facilitated), how therapies for them should be applied, or when they might be efficacious.

Methods

This article seeks to put forward cornerstone facts about neuroinflammation, with suggestions for future directions.

Results

Multiple scientific points about neuroinflammation still remain unaddressed. The main issue, however, is political. An expert body is needed that can sort through the myriad findings and provide consensus advice to agencies that fund basic and clinical research.

Discussion

Consortium approaches have shown immense value in other Alzheimer's disease research areas and are badly needed in neuroinflammation research. Lacking a consensus, neuroinflammation is just a very large number of isolated articles clamoring for attention.
Synaptic vesicle cycle and amyloid β: Biting the hand that feeds
Authors: Ovsepian Saak V. , O'Leary Valerie B. , Zaborszky Laszlo , Ntziachristos Vasilis , Dolly J. Oliver
Published: March 2018

The synaptic vesicle cycle (SVC) holds center stage in the biology of presynaptic terminals. Through recurrent exocytosis and endocytosis, it facilitates a sequence of events enabling chemical neurotransmission between functionally related neurons. As a fundamental process that links the interior of nerve cells with their environment, the SVC is also critical for signaling and provides an entry route for a range of pathogens and toxins, enabling detrimental effects. In Alzheimer's disease, the SVC is both the prime site of amyloid β production and toxicity. In this study, we discuss the emerging evidence for physiological and pathological effects of Aβ on various stages of the SVC, from postfusion membrane recovery to trafficking, docking, and priming of vesicles for fusion and transmitter release. Understanding of the mechanisms of Aβ interaction with the SVC within the unifying calcium hypothesis of aging and Alzheimer's disease should further elucidate the fundamental biology of the presynaptic terminal and reveal novel therapeutic targets for Alzheimer's disease and other age‐related dementias.
Wind-tunnel tests of the ERICA tiltrotor optimised air-intake
Authors: Gibertini G. , Zanotti A. , Campanardi G. , Auteri F. , Zagaglia D. , Crosta G.
Published: March 2018

Wind-tunnel tests were carried out to evaluate the performance of the Computational Fluid Dynamics (CFD)-based air-intake duct shape optimisation of the European platform tiltrotor ERICA. A 1/2.5 scale model including the nacelle, the external portion of the wing and two interchangeable internal ducts reproducing the baseline and optimised shape were manufactered to be tested in the large wind tunnel of Politecnico di Milano. Moreover, tests were carried out with the model equipped with rotating blade stubs. The comprehensive experimental campaign included tests reproducing different forward flight conditions of the aircraft including cruise and conversion phases. The evaluation of the internal duct performance was carried out by measuring total pressure losses and flow distortion by directional probes at the Aerodynamic Interface Plane (AIP). Additional pressure measurements were carried out on the internal surface of the duct to compare the pressure distributions along the air-intake. The experimental results confirmed that the optimised duct offers significantly improved performance with respect to the baseline configuration not only in cruise, representing the flight condition considered for the CFD optimisation, but also for the conversion condition. In particular, a remarkable reduction of the total pressure drop at the AIP was found with the optimised duct with the only exception for the stubs-on configuration in cruise. Indeed, the present investigation highlighted that the design of the blade stubs, particularly their length, represents a very critical aspect for air-intake performance tests due to significant disturbances that could be induced by the stubs’ wake on the internal duct flow.
Virer et tourner attributifs: De l'analyse quantitative des cooccurrences aux contrastes sémantiques
Authors: Lauwers Peter , Van Wettere Niek
Published: March 2018

La présente contribution porte sur tourner et virer, deux verbes attributifs (p. ex. elle a viré/tourné folle) qui ne sont pas encore reconnus pleinement par la tradition grammaticale normative ni dans les travaux des linguistes. Elle vise à établir le profil distributionnel / collocationnel de ces deux verbes qui semblent opérer dans le même domaine sémantico-fonctionnel, afin de mettre à nu les subtiles divergences au niveau de leur profil sémantique. Celles-ci sont rattachées à la sémantique de base du verbe lexical, laissant entrevoir dans ces emplois grammaticalisés des effets dus à la « persistance lexicale » (lexical persistence). Afin d'objectiver au maximum les profils distributionnels des deux verbes (et partant leur profil sémantique), les outils statistiques offerts par la méthode collostructionnelle (Stefanowitsch et Gries 2003; Gries et Stefanowitsch 2004) sont mis à profit. En outre, la combinaison de l'approche collocationnelle et de l'analyse morphosyntaxique amène à distinguer l'emploi proprement attributif des deux verbes d'un emploi directionnel abstrait homonymique (l'entreprise a viré solaire), qui est encore proche de la construction prépositionnelle (virer à / tourner à).
Differential Effects of Chlorhexidine Skin Cleansing Methods on Residual Chlorhexidine Skin Concentrations and Bacterial Recovery
Authors: Rhee Yoona , Palmer Louisa J. , Okamoto Koh , Gemunden Sean , Hammouda Khaled , Kemble Sarah K. , Lin Michael Y. , Lolans Karen , Fogg Louis , Guanaga Derek , Yokoe Deborah S. , Weinstein Robert A. , Frendl Gyorgy , Hayden Mary K. ,   for the Centers for Disease Control and Prevention Epicenter Program
Published: March 2018

BACKGROUND

Bathing intensive care unit (ICU) patients with 2% chlorhexidine gluconate (CHG)–impregnated cloths decreases the risk of healthcare-associated bacteremia and multidrug-resistant organism transmission. Hospitals employ different methods of CHG bathing, and few studies have evaluated whether those methods yield comparable results.

OBJECTIVE

To determine whether 3 different CHG skin cleansing methods yield similar residual CHG concentrations and bacterial densities on skin.

DESIGN

Prospective, randomized 2-center study with blinded assessment.

PARTICIPANTS AND SETTING

Healthcare personnel in surgical ICUs at 2 tertiary-care teaching hospitals in Chicago, Illinois, and Boston, Massachusetts, from July 2015 to January 2016.

INTERVENTION

Cleansing skin of one forearm with no-rinse 2% CHG-impregnated polyester cloth (method A) versus 4% CHG liquid cleansing with rinsing on the contralateral arm, applied with either non–antiseptic-impregnated cellulose/polyester cloth (method B) or cotton washcloth dampened with sterile water (method C).

RESULTS

In total, 63 participants (126 forearms) received method A on 1 forearm (n=63). On the contralateral forearm, 33 participants received method B and 30 participants received method C. Immediately and 6 hours after cleansing, method A yielded the highest residual CHG concentrations (2500 µg/mL and 1250 µg/mL, respectively) and lowest bacterial densities compared to methods B or C (P<.001).

CONCLUSION

In healthy volunteers, cleansing with 2% CHG-impregnated cloths yielded higher residual CHG concentrations and lower bacterial densities than cleansing with 4% CHG liquid applied with either of 2 different cloth types and followed by rinsing. The relevance of these differences to clinical outcomes remains to be determined.

Infect Control Hosp Epidemiol 2018;39:405–411
Empiric Antibiotic Prescribing Decisions Among Medical Residents: The Role of the Antibiogram
Authors: Tallman Gregory B. , Vilches-Tran Rowena A. , Elman Miriam R. , Bearden David T. , Taylor Jerusha E. , Gorman Paul N. , McGregor Jessina C.
Published: March 2018

OBJECTIVE

To assess general medical residents’ familiarity with antibiograms using a self-administered survey

DESIGN

Cross-sectional, single-center survey

PARTICIPANTS

Residents in internal medicine, family medicine, and pediatrics at an academic medical center

METHODS

Participants were administered an anonymous survey at our institution during regularly scheduled educational conferences between January and May 2012. Questions collected data regarding demographics, professional training; further open-ended questions assessed knowledge and use of antibiograms regarding possible pathogens, antibiotic regimens, and prescribing resources for 2 clinical vignettes; a series of directed, closed-ended questions followed. Bivariate analyses to compare responses between residency programs were performed.

RESULTS

Of 122 surveys distributed, 106 residents (87%) responded; internal medicine residents accounted for 69% of responses. More than 20% of residents could not accurately identify pathogens to target with empiric therapy or select therapy with an appropriate spectrum of activity in response to the clinical vignettes; correct identification of potential pathogens was not associated with selecting appropriate therapy. Only 12% of respondents identified antibiograms as a resource when prescribing empiric antibiotic therapy for scenarios in the vignettes, with most selecting the UpToDate online clinical decision support resource or The Sanford Guide. When directly questioned, 89% reported awareness of institutional antibiograms, but only 70% felt comfortable using them and only 44% knew how to access them.

CONCLUSIONS

When selecting empiric antibiotics, many residents are not comfortable using antibiograms as part of treatment decisions. Efforts to improve antibiotic use may benefit from residents being given additional education on both infectious diseases pharmacotherapy and antibiogram utilization.

Infect Control Hosp Epidemiol 2018;39:578–583
Callery pear (Pyrus calleryana) Response to Fire in a Managed Prairie Ecosystem
Authors: Warrix Adam R. , Marshall Jordan M.
Published: March 2018

Callery pear (Pyrus calleryana Decne.) was introduced to North America as an ornamental tree in the early 1900s. Due to widespread planting, P. calleryana has become common throughout the eastern United States and has invaded natural areas, especially disturbed areas. Prescribed fire is a common management technique in prairie ecosystems to mimic natural disturbances. We tested the effectiveness of prescribed fire as a control technique for P. calleryana in a managed prairie system. Fire top-killed all established P. calleryana individuals. However, these individuals responded to fire with 3 to 4 epicormic sprouts each. Similar sprouting behavior occurred in 2-yr-old seedlings. Exposed seeds, fruits, and 1-yr-old seedlings were killed by fire. Established P. calleryana were single-stemmed individuals before exposure to fire. After the prescribed fire, they all were multistemmed, which increased the potential flower-bearing stems within the prairie. We conclude that fire alone is not a suitable technique for managing P. calleryana invasion. Cut and herbicide application methods are labor-intensive. However, combining cut and spray methods with prescribed fire may be effective. Fire removes standing grass and forb biomass, leaving exposed P. calleryana stems, which would make locating individuals and directly applying herbicides easier.
Bulk scaling in wall-bounded and homogeneous vertical natural convection
Authors: Ng Chong Shen , Ooi Andrew , Lohse Detlef , Chung Daniel
Published: March 2018

Previous numerical studies on homogeneous Rayleigh–Bénard convection, which is Rayleigh–Bénard convection (RBC) without walls, and therefore without boundary layers, have revealed a scaling regime that is consistent with theoretical predictions of bulk-dominated thermal convection. In this so-called asymptotic regime, previous studies have predicted that the Nusselt number ($\mathit{Nu}$) and the Reynolds number ($\mathit{Re}$) vary with the Rayleigh number ($\mathit{Ra}$) according to $\mathit{Nu}\sim \mathit{Ra}^{1/2}$ and $\mathit{Re}\sim \mathit{Ra}^{1/2}$ at small Prandtl numbers ($\mathit{Pr}$). In this study, we consider a flow that is similar to RBC but with the direction of temperature gradient perpendicular to gravity instead of parallel to it; we refer to this configuration as vertical natural convection (VC). Since the direction of the temperature gradient is different in VC, there is no exact relation for the average kinetic dissipation rate, which makes it necessary to explore alternative definitions for $\mathit{Nu}$, $\mathit{Re}$ and $\mathit{Ra}$ and to find physical arguments for closure, rather than making use of the exact relation between $\mathit{Nu}$ and the dissipation rates as in RBC. Once we remove the walls from VC to obtain the homogeneous set-up, we find that the aforementioned $1/2$-power-law scaling is present, similar to the case of homogeneous RBC. When focusing on the bulk, we find that the Nusselt and Reynolds numbers in the bulk of VC too exhibit the $1/2$-power-law scaling. These results suggest that the $1/2$-power-law scaling may even be found at lower Rayleigh numbers if the appropriate quantities in the turbulent bulk flow are employed for the definitions of $\mathit{Ra}$, $\mathit{Re}$ and $\mathit{Nu}$. From a stability perspective, at low- to moderate-$\mathit{Ra}$, we find that the time evolution of the Nusselt number for homogenous vertical natural convection is unsteady, which is consistent with the nature of the elevator modes reported in previous studies on homogeneous RBC.
The oldest turritelline gastropods: from the Oxfordian (Upper Jurassic) of Kutch, India
Authors: Das Shiladri S. , Saha Sandip , Bardhan Subhendu , Mallick Sumanta , Allmon Warren D.
Published: March 2018

Turritellid gastropods are important components of many Cretaceous–Recent fossil marine faunas worldwide. Their shell is morphologically simple, making homoplasy widespread and phylogenetic analysis difficult, but fossil and living species can be recognized based on shell characters. For many decades, it has been the consensus that the oldest definite representatives of Turritellidae are from the Lower Cretaceous, and that pre-Cretaceous forms are homeomorphs. Some morphological characters of the present turritelline species resemble those of mathildoids, but many diagnostic characters clearly separate these two groups. We here describe and/or redescribe—based on examination of more than 2600 near complete specimens—four species from the Upper Jurassic Dhosa Oolite Member of the Chari Formation in Kutch, western India, and demonstrate that they are members of Turritellidae, subfamily Turritellinae, on the basis of diagnostic characters including apical sculptural ontogeny (obtained from SEM study), spiral sculpture, and growth line patterns. The four species are in order of abundance,Turritella jadavpuriensisMitra and Ghosh, 1979;Turritella amitavanew species;Turritella jhuraensisMitra and Ghosh, 1979, andTurritella dhosaensisnew species. The turritelline assemblages occur only on the northeastern flank of the Jhura dome (23°24’47.57”N, 69°36’09.26”E). Age of the Dhosa Oolite has recently been confirmed based on multiple ammonite species. All these points indicate that these fossils are the oldest record of the family Turritellidae—by almost 30 million years—in the world.
The Movimento Autonomista Bergamasco and the Lega Nord: continuities and discontinuities
Authors: Newth George
Published: March 2018

Studies of the Lega Nord (Northern League) have tended either to ignore the existence of earlier movements for regional autonomy in the 1950s – such as the Movimento Autonomista Bergamasco – or, on the contrary, assume continuities between the two movements at the expense of analysis of the significant discontinuities. This article argues that neither approach has been successful in capturing the importance of precursors to leghismo and that a more nuanced analysis is required. Focusing on the Movimento Autonomista Bergamasco (MAB) in its various incarnations between 1947 and 1970, I argue that the Bergamascan autonomists of the post-war period in many ways laid the foundations for Umberto Bossi’s Lega Nord; however, it is historically inaccurate to claim a direct line of continuity between mabismo and leghismo due to the differing contexts in which each movement operated: the critical junctures of the post-war era and post-tangentopoli respectively. What emerges from a closer analysis of the relationship between the two movements, as mabismo evolved into leghismo in the 1980s is a picture of regional autonomism distancing itself from the ideal of national unity and being increasingly drawn to secessionism.
Deep brain stimulation of the supero-lateral branch of the medial forebrain bundle does not lead to changes in personality in patients suffering from severe depression
Authors: Bewernick Bettina H. , Kilian Hannah M. , Schmidt Klaudius , Reinfeldt Ruth E. , Kayser Sarah , Coenen Volker A. , Markett Sebastian , Schlaepfer Thomas E.
Published: March 2018

Background

Reports of changes in patients’ social behavior during deep brain stimulation (DBS) raised the question whether DBS induces changes in personality. This study explored if (1) DBS is associated with changes in personality in patients suffering from treatment-resistant depression (TRD), (2) how personality dimensions and depression are associated, and (3) if TRD patients’ self-ratings of personality are valid.

Methods

TRD patients were assessed before DBS (n = 30), 6 months (t2, n = 21), 2 (t3, n = 17) and 5 years (t4, n = 11) after the initiation of DBS of the supero-lateral branch of the medial forebrain bundle (slMFB-DBS). Personality was measured with the NEO-Five-Factor Inventory (NEO-FFI), depression severity with Hamilton (HDRS), and Montgomery–Åsberg Depression Rating Scale (MADRS).

Results

Personality dimensions did not change with slMFB-DBS compared with baseline. Extraversion was negatively correlated with HDRS28 (r = −0.48, p < 0.05) and MADRS (r = −0.45, p < 0.05) at t2. Inter-rater reliability was high for the NEO-FFI at baseline (Cronbach's α = 0.74) and at t4 (α = 0.65). Extraversion [t(29) = −5.20; p < 0.001] and openness to experience [t(29) = −6.96; p < 0.001] differed statistically significant from the normative sample, and did not predict the antidepressant response.

Conclusions

slMFB-DBS was not associated with a change in personality. The severity of depression was associated with extraversion. Personality of TRD patients differed from the healthy population and did not change with response, indicating a possible scar effect. Self-ratings of personality seem valid to assess personality during TRD.
TELE-EXPERTISE SYSTEM BASED ON THE USE OF THE ELECTRONIC PATIENT RECORD TO SUPPORT REAL-TIME ANTIMICROBIAL USE
Authors: Morquin David , Ologeanu-Taddei Roxana , Koumar Yatrika , Reynes Jacques
Published: March 2018

Objectives: The aims of this study are (i) to present the design of a tele-expertise system, based on the telephone and electronic patient record (EPR), which supports the counseling of the infectious diseases specialist (IDS) for appropriate antimicrobial use, in a French University hospital; and (ii) to assess the diffusion of the system, the users’ adherence, and their perceived utility.

Methods: A prospective observational study was conducted to measure (i) the number and patterns of telephone calls for tele-expertise council, the number of initial and secondary assessments from the IDS and multidisciplinary meetings; (ii) the clinicians’ adherence rate to therapeutic proposals by the IDS and the number of clinical situations for which the IDS decided to move to bedside; and (iii) the perceived utility of the system by the medical managers of the most demanding departments.

Results: The review of patients’ records for 1 year period indicates that 87 percent of the therapeutic recommendations were fully followed. The adherence was high, despite the IDS moving to the bedside only in 6 percent of cases. Medical managers of the most demanding departments considered the system to be useful. Moreover, 6,994 tele-expertise notifications have been recorded into the EPR for 48 months.

Conclusions: The tele-expertise system is an original way to design information technology supported antimicrobial stewardship intervention based on the remote access to relevant information by the IDS and on the traceability of the medical counseling for the clinicians.
Initial laryngeal neutralisation in Tyrolean
Authors: Vietti Alessandro , Alber Birgit , Vogt Barbara
Published: March 2018

In the Southern Bavarian variety of Tyrolean, laryngeal contrasts undergo a typologically interesting process of neutralisation in word-initial position. We undertake an acoustic analysis of Tyrolean stops in word-initial, word-medial intersonorant and word-final contexts, as well as in obstruent clusters, investigating the role of the acoustic parameters VOT, prevoicing, closure duration and F0 and H1–H2* on following vowels in implementing contrast, if any. Results show that stops contrast word-medially via [voice] (supported by the acoustic cues of closure duration and F0), and are neutralised completely in word-final position and in obstruent clusters. Word-initially, neutralisation is subject to inter- and intraspeaker variability, and is sensitive to place of articulation. Aspiration plays no role in implementing laryngeal contrasts in Tyrolean.
Phonetic evidence for an iterative stress system: the issue of consonantal rhythm
Authors: Łukaszewicz Beata
Published: March 2018

In her study published in this journal, Newlin-Łukowicz (2012) calls into question the existence of bidirectional stress systems. The argument hinges on the failure to detect acoustic correlates of word-internal subsidiary stress in Polish, the language hitherto considered to be a classic example of metrical bidirectionality. This paper reappraises the issue, reporting on an acoustic study of paired five- and six-syllable words in Polish (e.g.ˎpomido′rowy–ˎpomiˎdoro-′wego). The results indicate that the words differ significantly with respect to relative consonant duration (PVI values) in the onset of the third syllable, depending on whether the syllable bears subsidiary stress (as in six-syllable words) or remains unstressed (as in five-syllable words). Similar effects are reported in the initial syllable, but not in the second syllable, which remains consistently unstressed. The conclusion is that Polish has iterative stress, corroborating its traditional description as having a bidirectional stress system.
Substance matters: a reply to Jardine (2016)
Authors: Pater Joe
Published: March 2018

Jardine (2016) claims that tonal phonology is more formally complex than the phonology of other segmental features, in that only tonal phonology goes beyond the class of weakly deterministic maps. He then goes on to argue that this formal distinction is superior to any available treatment in Optimality Theory. This reply points out that Jardine's formal distinction conflates attested and unattested tonal patterns, which can be distinguished in Optimality Theory, given a substantively defined constraint set.
Different strategies for breaking physical seed dormancy in field conditions in two fruit morphs of Vachellia caven (Fabaceae)
Authors: Ferreras Ana E. , Marcora Paula I. , Venier M. Paula , Funes Guillermo
Published: March 2018

Differences in fruit morphology among or within species might indicate differences in other regenerative traits, such as seed dormancy and germination. In species with physical dormancy (PY), environmental conditions are suggested to be responsible for dormancy break in field. Seeds of Vachellia caven have PY. This species exhibits two fruit morphs highly represented in Córdoba forests, Argentina: one is dehiscent and the other is indehiscent. In this study we performed a burial experiment with the aim to determine if the differences in V. caven fruit morphology were related to different patterns of PY break of their seeds in field conditions. We related these patterns to (1) environmental conditions that could influence the loss of PY, and (2) histological features of the lens zone. Seeds of both morphs exhibited dormancy break within 14 months of the start of the experiment, but with different patterns. The dehiscent morph showed an abrupt percentage of seeds that broke dormancy 14 months after the beginning of the experiment, probably after undergoing environmental changes similar to those suggested by the two-stage softening model. The indehiscent morph showed a gradual increase in seeds that broke dormancy, not clearly related to any of the environmental variables studied. No differences in seed coat structure of the lens zone were observed between morphs. The existence of both morphs could confer the species with higher possibilities of establishing and coping with environmental heterogeneity. Those characteristics contribute to the understanding of the success of this species in open and disturbed environments.
A pulmonary artery sling with a vascular ring in a toddler: an uncommon combination
Authors: Binsalamah Ziyad M. , Fraser Charles D. , Mery Carlos M.
Published: March 2018

Pulmonary artery slings and vascular rings are very rare congenital anomalies. It is even rarer to have both anomalies in the same setting. We present a case of a toddler who was diagnosed with a left pulmonary artery sling and a vascular ring as part of the screening process for the VACTERL association – co-occurrence of vertebral, anorectal, cardiac, tracheoesophageal, renal, and limb malformations. He underwent a successful surgical repair via median sternotomy and on cardiopulmonary bypass with an uneventful postoperative course.
Target and Non–target site Mechanisms Confer Resistance to Glyphosate in Canadian Accessions ofConyza canadensis
Authors: Page Eric R. , Grainger Christopher M. , Laforest Martin , Nurse Robert E. , Rajcan Istvan , Bae Jichul , Tardif François J.
Published: March 2018

Glyphosate-resistant populations ofConyza canadensishave been spreading at a rapid rate in Ontario, Canada, since first being documented in 2010. Determining the genetic relationship among existing Ontario populations is necessary to understand the spread and selection of the resistant biotypes. The objectives of this study were to: (1) characterize the genetic variation ofC. canadensisaccessions from the province of Ontario using simple sequence repeat (SSR) markers and (2) investigate the molecular mechanism (s) conferring resistance in these accessions. Ninety-eightC. canadensisaccessions were genotyped using 8 SSR markers. Germinable accessions were challenged with glyphosate to determine their dose response, and the sequences of 5-enolpyruvylshikimate-3-phosphate synthase genes 1 and 2 were obtained. Results indicate that a majority of glyphosate-resistant accessions from Ontario possessed a proline to serine substitution at position 106, which has previously been reported to confer glyphosate resistance in other crop and weed species. Accessions possessing this substitution demonstrated notably higher levels of resistance than non–target site resistant (NTSR) accessions from within or outside the growing region and were observed to form a subpopulation genetically distinct from geographically proximate glyphosate-susceptible and NTSR accessions. Although it is unclear whether other non–target site resistance mechanisms are contributing to the levels of resistance observed in target-site resistant accessions, these results indicate that, at a minimum, selection for Pro-106-Ser has occurred in addition to selection for non–target site resistance and has significantly enhanced the levels of resistance to glyphosate inC. canadensisaccessions from Ontario.
Evolutionary Adaptations of Palmer Amaranth (Amaranthus palmeri) to Nitrogen Fertilization and Crop Rotation History Affect Morphology and Nutrient-Use Efficiency
Authors: Bravo Washington , Leon Ramon G. , Ferrell Jason A. , Mulvaney Michael J. , Wood C. Wesley
Published: March 2018

Palmer amaranth control has become a major challenge for multiple cropping systems across the southeastern and midwestern United States. Despite extensive research on herbicide-resistance evolution, little research has been done exploring how Palmer amaranth might also be evolving other adaptive traits in response to different selection forces present in agricultural fields and the enrichment of soils with nutrients such as nitrogen. The objective of the present study was to determine whether Palmer amaranth populations have evolved different morphology and growth patterns in response to glyphosate use and fertilization history. Ten Palmer amaranth populations, including glyphosate-resistant (GR) and glyphosate-susceptible (GS) populations, were collected from different cropping systems with histories of high and low nitrogen fertilization in the states of Florida and Georgia. All populations were grown in pots filled with soil fertilized with either 0 or 40 kgNha−1, and their response to nitrogen was compared for morphological, growth, and nutrient-use traits. Populations differed in how they modified their morphology and growth in response to N, with major differences in traits such as foliar area, branch production, leaf shape, and canopy architecture. Populations with high nitrogen-fertilization histories had higher (>43%) nutrient-use efficiency (NUE) than populations with low nitrogen-fertilization histories. Similarly, GR populations have evolved higher NUE (>47%) and changed canopy architecture more than GS populations in response to nitrogen fertilization. The results of the present study highlight the importance of paying more attention to adaptations to cultural practices that might increase weediness and how genetic changes in traits involved in morphology and metabolism might favor compensatory mechanisms increasing the fitness of the population carrying herbicide-resistant traits.
Improving genetic prediction by leveraging genetic correlations among human diseases and traits
Authors: Maier Robert M. , Zhu Zhihong , Lee Sang Hong , Trzaskowski Maciej , Ruderfer Douglas M. , Stahl Eli A. , Ripke Stephan , Wray Naomi R. , Yang Jian , Visscher Peter M. , Robinson Matthew R.
Published: March 2018

Genomic prediction has the potential to contribute to precision medicine. However, to date, the utility of such predictors is limited due to low accuracy for most traits. Here theory and simulation study are used to demonstrate that widespread pleiotropy among phenotypes can be utilised to improve genomic risk prediction. We show how a genetic predictor can be created as a weighted index that combines published genome-wide association study (GWAS) summary statistics across many different traits. We apply this framework to predict risk of schizophrenia and bipolar disorder in the Psychiatric Genomics consortium data, finding substantial heterogeneity in prediction accuracy increases across cohorts. For six additional phenotypes in the UK Biobank data, we find increases in prediction accuracy ranging from 0.7% for height to 47% for type 2 diabetes, when using a multi-trait predictor that combines published summary statistics from multiple traits, as compared to a predictor based only on one trait.
Itch/β-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis
Authors: Infante Paola , Faedda Roberta , Bernardi Flavia , Bufalieri Francesca , Lospinoso Severini Ludovica , Alfonsi Romina , Mazzà Daniela , Siler Mariangela , Coni Sonia , Po Agnese , Petroni Marialaura , Ferretti Elisabetta , Mori Mattia , De Smaele Enrico , Canettieri Gianluca , Capalbo Carlo , Maroder Marella , Screpanti Isabella , Kool Marcel , Pfister Stefan M. , Guardavaccaro Daniele , Gulino Alberto , Di Marcotullio Lucia
Published: March 2018

Suppressor of Fused (SuFu), a tumour suppressor mutated in medulloblastoma, is a central player of Hh signalling, a pathway crucial for development and deregulated in cancer. Although the control of Gli transcription factors by SuFu is critical in Hh signalling, our understanding of the mechanism regulating this key event remains limited. Here, we show that the Itch/β-arrestin2 complex binds SuFu and induces its Lys63-linked polyubiquitylation without affecting its stability. This process increases the association of SuFu with Gli3, promoting the conversion of Gli3 into a repressor, which keeps Hh signalling off. Activation of Hh signalling antagonises the Itch-dependent polyubiquitylation of SuFu. Notably, different SuFu mutations occurring in medulloblastoma patients are insensitive to Itch activity, thus leading to deregulated Hh signalling and enhancing medulloblastoma cell growth. Our findings uncover mechanisms controlling the tumour suppressive functions of SuFu and reveal that their alterations are implicated in medulloblastoma tumorigenesis.
Surface energy of strained amorphous solids
Authors: Schulman Rafael D. , Trejo Miguel , Salez Thomas , Raphaël Elie , Dalnoki-Veress Kari
Published: March 2018

Surface stress and surface energy are fundamental quantities which characterize the interface between two materials. Although these quantities are identical for interfaces involving only fluids, the Shuttleworth effect demonstrates that this is not the case for most interfaces involving solids, since their surface energies change with strain. Crystalline materials are known to have strain-dependent surface energies, but in amorphous materials, such as polymeric glasses and elastomers, the strain dependence is debated due to a dearth of direct measurements. Here, we utilize contact angle measurements on strained glassy and elastomeric solids to address this matter. We show conclusively that interfaces involving polymeric glasses exhibit strain-dependent surface energies, and give strong evidence for the absence of such a dependence for incompressible elastomers. The results provide fundamental insight into our understanding of the interfaces of amorphous solids and their interaction with contacting liquids.
Nitrogen fixation sustained productivity in the wake of the Palaeoproterozoic Great Oxygenation Event
Authors: Luo Genming , Junium Christopher K. , Izon Gareth , Ono Shuhei , Beukes Nicolas J. , Algeo Thomas J. , Cui Ying , Xie Shucheng , Summons Roger E.
Published: March 2018

The marine nitrogen cycle is dominated by redox-controlled biogeochemical processes and, therefore, is likely to have been revolutionised in response to Earth-surface oxygenation. The details, timing, and trajectory of nitrogen cycle evolution, however, remain elusive. Here we couple nitrogen and carbon isotope records from multiple drillcores through the Rooihoogte–Timeball Hill Formations from across the Carletonville area of the Kaapvaal Craton where the Great Oxygenation Event (GOE) and its aftermath are recorded. Our data reveal that aerobic nitrogen cycling, featuring metabolisms involving nitrogen oxyanions, was well established prior to the GOE and that ammonium may have dominated the dissolved nitrogen inventory. Pronounced signals of diazotrophy imply a stepwise evolution, with a temporary intermediate stage where both ammonium and nitrate may have been scarce. We suggest that the emergence of the modern nitrogen cycle, with metabolic processes that approximate their contemporary balance, was retarded by low environmental oxygen availability.
Topological hybrid silicon microlasers
Authors: Zhao Han , Miao Pei , Teimourpour Mohammad H. , Malzard Simon , El-Ganainy Ramy , Schomerus Henning , Feng Liang
Published: March 2018

Topological physics provides a robust framework for strategically controlling wave confinement and propagation dynamics. However, current implementations have been restricted to the limited design parameter space defined by passive topological structures. Active systems provide a more general framework where different fundamental symmetry paradigms, such as those arising from non-Hermiticity and nonlinear interaction, can generate a new landscape for topological physics and its applications. Here, we bridge this gap and present an experimental investigation of an active topological photonic system, demonstrating a topological hybrid silicon microlaser array respecting the charge-conjugation symmetry. The created new symmetry features favour the lasing of a protected zero mode, where robust single-mode laser action in the desired state prevails even with intentionally introduced perturbations. The demonstrated microlaser is hybrid implemented on a silicon-on-insulator substrate, and is thereby readily suitable for integrated silicon photonics with applications in optical communication and computing.
The CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes
Authors: Nieto-Fontarigo J. J. , Salgado F. J. , San-José M. E. , Cruz M. J. , Casas-Fernández A. , Gómez-Conde M. J. , Valdés-Cuadrado L. , García-González M. Á. , Arias P. , Nogueira M. , González-Barcala F. J.
Published: March 2018

LPS-ligation to CD14/TLR-4 on monocytes/macrophages triggers the production of IL-12-family cytokines. IL12/18 promote TH1-differentiation, counteracting the TH2-driven asthma. Therefore, CD14 modulation could alter the TH2-differentiation and should be taken into account when studying asthma. To analyse the alteration in CD14 levels and its association with CD14 (−159 C/T) SNP (rs2569190) in Caucasian adults with stable allergic asthma, we performed a cross-sectional study (277 healthy subjects vs. 277 patients) where clinical parameters, CD14 values and the CD14 (−159 C/T) SNP were studied. Apart from typical biomarkers, we found an increment of neuron-specific enolase (NSE) in allergic asthma, probably linked to monocyte activity. Indeed, we evidenced increased monocyte numbers, but lower CD14 expression and normalised sCD14 values in patients. Moreover, we noticed an association of the T allele (P = 0.0162) and TT genotype (P = 0.0196) of the CD14 SNP with a decreased risk of allergic asthma and augmented sCD14 levels. In conclusion, monocyte CD14 expression and normalized sCD14 values were reduced in stable state asthmatics, and this could be related to the presence of an expanded CD14low monocyte subset. This study also demonstrates that the CD14 (−159 C/T) polymorphism is a risk factor for moderate-severe allergic asthma in adult Caucasians.
Measurement-device-independent quantum key distribution via quantum blockade
Authors: Zhou Yi-Heng , Yu Zong-Wen , Li Ao , Hu Xiao-Long , Jiang Cong , Wang Xiang-Bin
Published: March 2018

Efficiency in measurement-device-independent quantum key distribution(MDI-QKD) can be improved not only by the protocol, but also single-photon sources. We study the behavior of MDI-QKD with statistical fluctuation using quantum blockade source. Numerical simulation for a type of 4-intensity protocol shows that, after parameter optimization, this source can improve the final key rate by 100 times compared with traditional weak coherent state sources.
Machine Learning-based Individual Assessment of Cortical Atrophy Pattern in Alzheimer’s Disease Spectrum: Development of the Classifier and Longitudinal Evaluation
Authors: Lee Jin San , Kim Changsoo , Shin Jeong-Hyeon , Cho Hanna , Shin Dae-seock , Kim Nakyoung , Kim Hee Jin , Kim Yeshin , Lockhart Samuel N. , Na Duk L. , Seo Sang Won , Seong Joon-Kyung
Published: March 2018

To develop a new method for measuring Alzheimer’s disease (AD)-specific similarity of cortical atrophy patterns at the individual-level, we employed an individual-level machine learning algorithm. A total of 869 cognitively normal (CN) individuals and 473 patients with probable AD dementia who underwent high-resolution 3T brain MRI were included. We propose a machine learning-based method for measuring the similarity of an individual subject’s cortical atrophy pattern with that of a representative AD patient cohort. In addition, we validated this similarity measure in two longitudinal cohorts consisting of 79 patients with amnestic-mild cognitive impairment (aMCI) and 27 patients with probable AD dementia. Surface-based morphometry classifier for discriminating AD from CN showed sensitivity and specificity values of 87.1% and 93.3%, respectively. In the longitudinal validation study, aMCI-converts had higher atrophy similarity at both baseline (p < 0.001) and first year visits (p < 0.001) relative to non-converters. Similarly, AD patients with faster decline had higher atrophy similarity than slower decliners at baseline (p = 0.042), first year (p = 0.028), and third year visits (p = 0.027). The AD-specific atrophy similarity measure is a novel approach for the prediction of dementia risk and for the evaluation of AD trajectories on an individual subject level.
Damage to epitaxial GaN layer on Al2O3 by 290-MeV 238U32+ ions irradiation
Authors: Zhang L. Q. , Zhang C. H. , Li J. J. , Meng Y. C. , Yang Y. T. , Song Y. , Ding Z. N. , Yan T. X.
Published: March 2018

Micro-structural characteristics and electrical properties of an n-type GaN epilayer on Al2O3 irradiated by 290-MeV 238U32+ ions to various fluences were investigated using atomic force microscopy (AFM), scanning electron microscopy (SEM), high-resolution X-ray diffraction (HRXRD), and Raman scattering spectroscopy. AFM images show that the nano-hillocks generated, and the diameter and density of the nano-hillocks, increase obviously with increasing ion fluence, accompanied by an increase in surface roughness. SEM images display that the Al, O, and C elements appear on the GaN surface, along with a spiral-like, layered volcanic-cone structure formed at the highest-fluence irradiation. HRXRD reveals that the dislocation density increases, as the lattices gradually expand, and that Ga2O3 was produced with increasing ion fluence. Raman scattering spectra show that no N and Ga vacancies were produced, the free-carrier concentration decreases, while its mobility first increases and then exhibits a significant reduction with increasing ion fluence.
Detection of the Malaria causing Plasmodium Parasite in Saliva from Infected Patients using Topoisomerase I Activity as a Biomarker
Authors: Hede Marianne Smedegaard , Fjelstrup Søren , Lötsch Felix , Zoleko Rella Manego , Klicpera Anna , Groger Mirjam , Mischlinger Johannes , Endame Lilian , Veletzky Luzia , Neher Ronja , Simonsen Anne Katrine Wrist , Petersen Eskild , Mombo-Ngoma Ghyslain , Stougaard Magnus , Ho Yi-Ping , Labouriau Rodrigo , Ramharter Michael , Knudsen Birgitta Ruth
Published: March 2018

Malaria is among the major threats to global health with the main burden of disease being in rural areas of developing countries where accurate diagnosis based on non-invasive samples is in high demand. We here present a novel molecular assay for detection of malaria parasites based on technology that may be adapted for low-resource settings. Moreover, we demonstrate the exploitation of this assay for detection of malaria in saliva. The setup relies on pump-free microfluidics enabled extraction combined with a DNA sensor substrate that is converted to a single-stranded DNA circle specifically by topoisomerase I expressed by the malaria causing Plasmodium parasite. Subsequent rolling circle amplification of the generated DNA circle in the presence of biotin conjugated deoxynucleotides resulted in long tandem repeat products that was visualized colorimetrically upon binding of horse radish peroxidase (HRP) and addition of 3,3′,5,5′-Tetramethylbenzidine that was converted to a blue colored product by HRP. The assay was directly quantitative, specific for Plasmodium parasites, and allowed detection of Plasmodium infection in a single drop of saliva from 35 out of 35 infected individuals tested. The results could be determined directly by the naked eye and documented by quantifying the color intensity using a standard paper scanner.