bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamics in Quaternary Ionic Liquids with Non-Flexible Anions: Insights from Mechanical Spectroscopy
Authors: Palumbo Oriele , Paolone Annalisa , Philippi Frederik , Rauber Daniel , Welton Tom
Published: July 2023

The present work investigates how mechanical properties and ion dynamics in ionic liquids (ILs) can be affected by ILs’ design while considering possible relationships between different mechanical and transport properties. Specifically, we study mechanical properties of quaternary ionic liquids with rigid anions by means of Dynamical Mechanical Analysis (DMA). We are able to relate the DMA results to the rheological and transport properties provided by viscosity, conductivity, and diffusion coefficient measurements. A good agreement is found in the temperature dependence of different variables described by the Vogel−Fulcher−Tammann model. In particular, the mechanical spectra of all the measured liquids showed the occurrence of a relaxation, for which the analysis suggested its attribution to a diffusive process, which becomes evident when the ion dynamics are not affected by the fast structural reorganization of flexible anions on a local level.
The CXCL13 Index as a Predictive Biomarker for Activity in Clinically Isolated Syndrome
Authors: Pike Steven C. , Gilli Francesca , Pachner Andrew R.
Published: July 2023

Multiple sclerosis (MS) is a clinically heterogenous disease. Currently, we cannot identify patients with more active disease who may potentially benefit from earlier interventions. Previous data from our lab identified the CXCL13 index (ICXCL13), a measure of intrathecal production of CXCL13, as a potential biomarker to predict future disease activity in MS patients two years after diagnosis. Patients with clinically isolated syndrome (CIS) or radiologically isolated syndrome (RIS) underwent a lumbar puncture and blood draw, and the ICXCL13 was determined. They were then followed for at least 5 years for MS activity. Patients with high ICXCL13 were more likely to convert to clinically definite MS (82.4%) compared to those with low ICXCL13 (10.0%). The data presented below demonstrate that this predictive ability holds true in CIS and RIS patients, and for at least five years compared to our initial two-year follow-up study. These data support the concept that ICXCL13 has the potential to be used to guide immunomodulatory therapy in MS.
Inflammation of Dry Eye Syndrome: A Cellular Study of the Epithelial and Macrophagic Involvement of NFAT5 and RAGE
Authors: Henrioux Fanny , Navel Valentin , Belville Corinne , Charnay Coline , Antoine Audrey , Chiambaretta Frédéric , Sapin Vincent , Blanchon Loïc
Published: July 2023

Dry eye inflammation is a key step in a vicious circle and needs to be better understood in order to break it. The goals of this work were to, first, characterize alarmins and cytokines released by ocular surface cells in the hyperosmolar context and, second, study the role of NFAT5 in this process. Finally, we studied the potential action of these alarmins in ocular surface epithelial cells and macrophages via RAGE pathways. HCE and WKD cell lines were cultured in a NaCl-hyperosmolar medium and the expression of alarmins (S100A4, S100A8, S100A9, and HMGB1), cytokines (IL6, IL8, TNFα, and MCP1), and NFAT5 were assessed using RT-qPCR, ELISA and multiplex, Western blot, immunofluorescence, and luciferase assays. In selected experiments, an inhibitor of RAGE (RAP) or NFAT5 siRNAs were added before the hyperosmolar stimulations. HCE and WKD cells or macrophages were treated with recombinant proteins of alarmins (with or without RAP) and analyzed for cytokine expression and chemotaxis, respectively. Hyperosmolarity induced epithelial cell inflammation depending on cell type. NFAT5, but not RAGE or alarmins, participated in triggering epithelial inflammation. Furthermore, the release of alarmins induced macrophage migration through RAGE. These in vitro results suggest that NFAT5 and RAGE have a role in dry eye inflammation.
Deciphering Molecular Mechanisms Governing the Reproductive Molt of Macrobrachium nipponense: A Transcriptome Analysis of Ovaries across Various Molting Stages
Authors: Yuan Huwei , Gao Zijian , Cai Pengfei , Zhang Wenyi , Jin Shubo , Jiang Sufei , Xiong Yiwei , Gong Yongsheng , Qiao Hui , Fu Hongtuo
Published: July 2023

The relationship between molting and reproduction has received more attention in economically important crustacean decapods. Molting and reproduction are synergistic events in Macrobrachium nipponense, but the molecular regulatory mechanisms behind them are unclear. In the current study, we performed Illumina sequencing for the ovaries of M. nipponense during the molt cycle (pre-molting, Prm; mid-molting, Mm; and post-molting, Pom). A total of 66.57 Gb of transcriptome data were generated through sequencing, resulting in the identification of 105,149 unigenes whose alignment ratio with the reference genome exceeded 87.57%. Differentially expressed genes (DEGs) were annotated through the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases for gene classification and pathway analysis. A total of twenty-six molt-related DEGs were found, and their expression patterns were examined across various molting stages. The KEGG enrichment analysis revealed that the key pathways involved in regulating the molting process of M. nipponense primarily include the mTOR, insect hormone biosynthesis, TGF-beta, and Wnt signaling pathways. Our transcriptomic data suggest that these pathways crosstalk with each other to regulate the synthesis and degradation of ecdysone throughout the molt cycle. The current study has deepened our understanding of the molecular mechanisms of crustacean molting and will serve as a basis for future studies of crustaceans and other molting animals.
Positive Allosteric Modulator of SERCA Pump NDC-1173 Exerts Beneficial Effects in Mouse Model of Alzheimer’s Disease
Authors: Dahl Russell , Moore Amanda C. , Knight Caitlynn , Mauger Colleen , Zhang Hua , Schiltz Gary E. , Koss Wendy A. , Bezprozvanny Ilya
Published: July 2023

Alzheimer’s disease (AD) is an irreversible neurodegenerative disease that affects millions of people worldwide. AD does not have a cure and most drug development efforts in the AD field have been focused on targeting the amyloid pathway based on the “amyloid cascade hypothesis”. However, in addition to the amyloid pathway, substantial evidence also points to dysregulated neuronal calcium (Ca2+) signaling as one of the key pathogenic events in AD, and it has been proposed that pharmacological agents that stabilize neuronal Ca2+ signaling may act as disease-modifying agents in AD. In previous studies, we demonstrated that positive allosteric regulators (PAMs) of the Sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) pump might act as such Ca2+ stabilizing agents. In the present study, we report the development of a novel SERCA PAM agent, compound NDC-1173. To test the effectiveness of this compound, we performed behavioral studies with the APP/PS1 transgenic AD mouse model. We also evaluated effects of this compound on expression of endoplasmic reticulum (ER) stress genes in the hippocampus of APP/PS1 mice. The results of this study support the hypothesis that the SERCA pump is a potential novel therapeutic drug target and that NDC-1173 is a promising lead molecule for developing disease-modifying agents in AD.
Examining the Drivers of E-Commerce Adoption by Moroccan Firms: A Multi-Model Analysis
Authors: Ben Youssef Adel , Dahmani Mounir
Published: July 2023

In the context of an increasingly digitized global marketplace, this study seeks to shed light on its adoption in developing countries, focusing on Morocco. Applying logit, probit, and conditional mixed-process probit models to a sample of 807 Moroccan firms, we identify key factors that influence e-commerce adoption. The results show that younger, innovation-driven firms and those with a highly educated workforce tend to adopt e-commerce more readily. However, digital skills required in hiring do not significantly affect adoption, suggesting a complex relationship between digital skills and e-commerce use. The results also show that firms that are active on digital platforms and engage in innovative practices are more likely to adopt e-commerce. Therefore, this study argues for the need to improve digital skills training and for firms to establish a presence on digital platforms and promote innovation. On the policy front, the study suggests the promotion of supportive policies such as financial assistance, improved Internet infrastructure, and robust regulatory frameworks. As an important starting point for future research, these findings underscore the complexities of e-commerce adoption in Morocco and can guide further research, particularly in the context of similar emerging economies.
A Practical Hybrid IoT Architecture with Deep Learning Technique for Healthcare and Security Applications
Authors: Vu Viet Q. , Tran Minh-Quang , Amer Mohammed , Khatiwada Mahesh , Ghoneim Sherif S. M. , Elsisi Mahmoud
Published: July 2023

Facial mask detection technology has become increasingly important even beyond the context of the COVID-19 pandemic. Along with the advancement in facial recognition technology, face mask detection has become a crucial feature for various applications. This paper introduces an Internet of Things (IoT) architecture based on a developed deep learning algorithm named You Only Look Once (YOLO) to keep society healthy, and secured, and collect data for future research. The proposed paradigm is built on the basis of economic consideration and is easy to implement. Yet, the used YOLOv4-tiny is one of the fastest object detection models to exist. A mask detection camera (MaskCam) that leverages the computing power of NVIDIA’s Jetson Nano edge nanodevices was built side by side with a smart camera application to detect a mask on the face of an individual. MaskCam distinguishes between mask wearers, those who are not wearing masks, and those who are not wearing masks properly according to MQTT protocol. Furthermore, a self-developed web browsing application comes with the MaskCam system to collect and visualize statistics for qualitative and quantitative analysis. The practical results demonstrate the superiority and effectiveness of the proposed smart mask detection system. On the one hand, YOLOv4-full obtained the best results even at smaller resolutions, although the frame rate is too small for real-time use. On the other hand, it is twice as fast as the other detection models, regardless of the quality of detection. Consequently, inferences may be run more frequently over the entire video sequence, resulting in more accurate output.
Online Professional Development on Educational Neuroscience in Higher Education Based on Design Thinking
Authors: Mystakidis Stylianos , Christopoulos Athanasios , Fragkaki Maria , Dimitropoulos Konstantinos
Published: July 2023

Higher education teaching staff members need to build a scientifically accurate and comprehensive understanding of the function of the brain in learning as neuroscience evidence can constitute a way to optimize teaching and achieve learning excellence. An international consortium developed a professional development six-module course on educational neuroscience and online community of practice by applying design thinking. A mixed methods research design was employed to investigate the attitudes of thirty-two (N = 32) participating academics using a survey comprising eleven closed and open questions. Data analysis methods included descriptive statistics, correlation, generalized additive model and grounded theory. The overall evaluation demonstrated a notable satisfaction level with regard to the quality of the course. Given the power of habits, mentoring and peer interactions are recommended to ensure the effective integration of theoretical neuroscientific evidence into teaching practice.
Classification of Benign and Malignant Renal Tumors Based on CT Scans and Clinical Data Using Machine Learning Methods
Authors: Xu Jie , He Xing , Shao Wei , Bian Jiang , Terry Russell
Published: July 2023

Up to 20% of renal masses ≤4 cm is found to be benign at the time of surgical excision, raising concern for overtreatment. However, the risk of malignancy is currently unable to be accurately predicted prior to surgery using imaging alone. The objective of this study is to propose a machine learning (ML) framework for pre-operative renal tumor classification using readily available clinical and CT imaging data. We tested both traditional ML methods (i.e., XGBoost, random forest (RF)) and deep learning (DL) methods (i.e., multilayer perceptron (MLP), 3D convolutional neural network (3DCNN)) to build the classification model. We discovered that the combination of clinical and radiomics features produced the best results (i.e., AUC [95% CI] of 0.719 [0.712–0.726], a precision [95% CI] of 0.976 [0.975–0.978], a recall [95% CI] of 0.683 [0.675–0.691], and a specificity [95% CI] of 0.827 [0.817–0.837]). Our analysis revealed that employing ML models with CT scans and clinical data holds promise for classifying the risk of renal malignancy. Future work should focus on externally validating the proposed model and features to better support clinical decision-making in renal cancer diagnosis.
Tribe Acalyptaini (Hemiptera: Tingidae: Tinginae) Revisited: Can Apomorphies in Secondary and Tertiary Structures of 18S rRNA Length-Variable Regions (LVRs) Support Tribe Validity?
Authors: Lis Barbara , Domagała Paweł J. , Lis Jerzy A.
Published: July 2023

The lace bug tribe Acalyptaini (Tingidae: Tinginae) includes five genera, Acalypta, Derephysia, Dictyonota, Kalama, and Recaredus, and it was recently resurrected based on morphological and karyological characters. We aimed to validate the distinctiveness of this tribe using 18S rDNA sequences, which have not been used in previous Tingidae phylogenomic studies. Our results confirmed the monophyly of the tribe. Moreover, the monophyly of the subfamily Cantacaderinae and its basal position within the family Tingidae were indicated, as well as the position of the tribe Litadeini as sister to all other Tinginae. In addition, we attempted to determine the apomorphic morpho-molecular characters in the secondary and tertiary structures of length-variable regions of the 18S rRNA sequences of the analysed species. The results showed that two LVRs (LVR X and LVR L) of the hypervariable region V4 exhibited significant variability in the number of nucleotides and could be considered for apomorphic recognition.
Establishing an Integrated Pest Management Program for Coffee Berry Borer (Hypothenemus hampei) in Hawaii and Puerto Rico Coffee Agroecosystems: Achievements and Challenges
Authors: Aristizábal Luis F. , Johnson Melissa A. , Mariño Yobana A. , Bayman Paul , Wright Mark G.
Published: July 2023

Coffee berry borer (CBB) is the most serious insect pest of coffee worldwide, causing significant reductions in yield and quality. Following the introduction of CBB to Puerto Rico (2007) and Hawaii (2010), researchers, extension agents, industry representatives, and coffee growers have worked together to develop an integrated pest management (IPM) program that is both effective and economically feasible for each island. Since the introduction of the IPM program in Hawaii, research efforts have led to a better understanding of CBB population dynamics, as well as optimized monitoring, cultural practices, and commercial Beauveria bassiana applications. As a result of these efforts, a substantial reduction in average CBB infestation and an increase in coffee yields and quality have been documented in Hawaii over the last decade. However, significant challenges remain in addressing high production and labor costs, limited availability of labor, and a lack of training for field workers in both regions. Although considerable effort has gone into research to support CBB IPM in Hawaii and Puerto Rico, the adoption of these strategies by coffee farmers needs to be increased. More diversified methods of outreach and education are needed to reach growers in rural, isolated areas. Significant gaps exist in the ability and willingness of growers and workers to access and digest information online, emphasizing the importance of on-farm workshops and farmer-to-farmer teaching. Additional methods of training are needed to help coffee farmers and field workers learn how to properly conduct cultural controls and optimize the use of biological control agents such as B. bassiana.
Seasonal Dynamics of Aphid Flights and Cotton Leafroll Dwarf Virus Spread in Alabama
Authors: Mahas Jessica B. , Ray Charles , Kesheimer Adam , Conner Kassie , Jacobson Alana L.
Published: July 2023

Cotton leafroll dwarf virus (CLRDV) is an introduced Polerovirus (Family: Solemoviridae) of cotton, Gossypium hirsutum L., in the U.S. The only vector known to transmit this virus to cotton is the cotton aphid, Aphis gossypii Glover; however, there are seven other species of aphids (Hemiptera: Aphididae) reported to colonize cotton in the southeastern U.S.: Protaphis middletonii (Thomas), Rhopalosiphum rufiabdominale (Sasaki), Aphis craccivora Koch, Macrosiphum euphorbiae Thomas, Myzus persicae (Sulzer), Smythurodes betae Westwood, and Aphis fabae Scopoli. Little to no information is available on annual population dynamics of these species in the southeastern U.S. The timing of CLRDV spread to cotton plantings is also unknown. The objective of this study was to monitor the population dynamics of eight cotton-feeding aphid species concurrent with the spread of CLRDV at three different locations in Alabama. Aphids were monitored weekly for two years with yellow pan traps, and sentinel plants were deployed weekly to monitor CLRDV spread throughout the cotton-growing season. During the two years, most CLRDV spread at all locations occurred when A. gossypii was actively dispersing in the field. Early season spread at sites in south and central Alabama, when A. gossypii was not abundant, suggests additional aphid vectors are possible.
Long-Term Physical Activity Effectively Reduces the Consumption of Antihypertensive Drugs: A Randomized Controlled Trial
Authors: di Cagno Alessandra , Fiorilli Giovanni , Buonsenso Andrea , Di Martino Giulia , Centorbi Marco , Angiolillo Antonella , Calcagno Giuseppe , Komici Klara , Di Costanzo Alfonso
Published: July 2023

Background: Although physical activity (PA) has been shown to enhance hypertension control, the impact of exercise on the potential decrease of the use of antihypertensive medications remains inadequately researched. Aim: The aim was to assess the impact of a two-year PA on the medication requirements of individuals with hypertension. Methods: A clinical trial was conducted, involving 130 participants with essential hypertension who took at least one antihypertensive medication. Participants were randomly assigned to either a control group (CG n = 65) or an experimental group (EG n = 65) that underwent a 24-month supervised PA program based on a combination of aerobic and resistance training. The antihypertensive drug load for each participant was determined by adding the ratios of the prescribed daily dose (PDD) to the defined daily dose (DDD) for all antihypertensive medications taken by the participants. The outcome measures were evaluated at 0, 6, 12, 18, and 24 months. Results: A total of 76 participants completed the 24-month assessment, and RM-ANOVA revealed a significantly lower antihypertensive drug load in the EG compared to the CG at 18 (p < 0.017) and 24 months (p < 0.003). Conclusion: A long-term PA program can decrease the antihypertensive drug load in older adults with essential hypertension. The trend of improvement regarding the EG drug load intake and the trend of CG drug load increase, although not significant over time, results in a significant difference between the groups at 18 months and an even greater difference at 24 months. This trend certifies the protective value of PA against the aging process and its related health risk factors.
Pharmacogenomics of Old and New Immunosuppressive Drugs for Precision Medicine in Kidney Transplantation
Authors: Turolo Stefano , Edefonti Alberto , Syren Marie Luise , Montini Giovanni
Published: July 2023

Kidney transplantation is the preferred therapeutic option for end-stage kidney disease, but, despite major therapeutic advancements, allograft rejection continues to endanger graft survival. Every patient is unique due to his or her clinical history, drug metabolism, genetic background, and epigenetics. For this reason, examples of “personalized medicine” and “precision medicine” have steadily increased in recent decades. The final target of precision medicine is to maximize drug efficacy and minimize toxicity for each individual patient. Immunosuppressive drugs, in the setting of kidney transplantation, require a precise dosage to avoid either adverse events (overdosage) or a lack of efficacy (underdosage). In this review, we will explore the knowledge regarding the pharmacogenomics of the main immunosuppressive medications currently utilized in kidney transplantation. We will focus on clinically relevant pharmacogenomic data, that is, the polymorphisms of the genes that metabolize immunosuppressive drugs.
COVID-19 and Its Cardiovascular Effects: Risk Factors, Prevention and Management
Authors: Sardu Celestino , Marfella Raffele
Published: July 2023

Coronavirus disease 2019 (COVID-19) is caused by a positive-stranded, single-stranded RNA virus, which is a member of the Sarbecovirus subgenus (beta-CoV lineage B) [...]
Long-Term Outcomes after Multimodal Treatment for Clival Chordoma: Efficacy of the Endonasal Transclival Approach with Early Adjuvant Radiation Therapy
Authors: Yoo Hyun Dong , Chung Jong Chul , Park Ki Seok , Chung Seung Young , Park Moon Sun , Ryu Seungjun , Kim Seong Min
Published: July 2023

This study investigates the long-term outcomes of clival chordoma patients treated with the endonasal transclival approach (ETCA) and early adjuvant radiation therapy. A retrospective review of 17 patients (2002–2013) showed a 10-year progression-free survival (PFS) rate of 67.4%, with the ETCA group showing fewer progressions and cranial neuropathies than those treated with combined approaches. The ETCA, a minimally invasive technique, provided a similar extent of resection compared to conventional skull-base approaches and enabled safe delivery of high-dose adjuvant radiotherapy. The findings suggest that ETCA is an effective treatment for centrally located clival chordomas.
Congenital Cytomegalovirus and Hearing Loss: The State of the Art
Authors: Aldè Mirko , Binda Sandro , Primache Valeria , Pellegrinelli Laura , Pariani Elena , Pregliasco Fabrizio , Di Berardino Federica , Cantarella Giovanna , Ambrosetti Umberto
Published: July 2023

In developed countries, congenital cytomegalovirus (cCMV) infection is the most common congenital viral infection, representing the leading non-genetic cause of sensorineural hearing loss (HL). Diagnosis of cCMV infection can be performed by detection of CMV DNA in urine or saliva within 2–3 weeks after birth, or later in dried blood samples on the Guthrie card. Currently, there are many controversies regarding the preventive, diagnostic, and therapeutic approaches to cCMV infection. HL secondary to cCMV is highly variable in onset, side, degree, audiometric configuration, and threshold changes over time. Therefore, it is of paramount importance to perform a long and thorough audiological follow-up in children with cCMV infection to ensure early identification and prompt treatment of progressive and/or late-onset HL. Early cochlear implantation appears to be a valid solution not only for children with bilateral profound HL, but also for those with single-sided deafness, improving localization ability and understanding speech in noisy environments. Moreover, the decision to apply a unilateral cochlear implant in children with cCMV is strengthened by the non-negligible possibility of hearing deterioration of the contralateral ear over time.
Impacts of Whole-Body Vibration on Muscle Strength, Power, and Endurance in Older Adults: A Systematic Review and Meta-Analysis
Authors: Gonçalves de Oliveira Raphael , Coutinho Heloísa Maria Elaine Licha , Martins Maiara Naldi Mansano , Bernardo-Filho Mario , de Sá-Caputo Danúbia da Cunha , Campos de Oliveira Laís , Taiar Redha
Published: July 2023

Background: Randomized clinical trials (RCTs) were conducted to identify the effectiveness of whole-body vibration (WBV) on strength, power, and muscular endurance in older adults. However, the results of different studies are contradictory. Objective: To verify the impacts of the WBV on strength, power, and muscular endurance in older adults. Methods: The search was carried out in PubMed, Embase, CENTRAL, CINAHL, SPORTDiscus, Web of Science, LILACS and PEDro databases. Methodological quality was assessed using the PEdro scale. Meta-analysis calculations were performed using the standardized mean difference, comparing WBV with control groups and WBV with other types of exercise. Results: Thirty-four studies were included in the current systematic review. Most studies (56%) had low methodological quality (PEDro score < 6). WBV, compared with control groups, has significant effects on muscle strength of knee extensors and flexors, lower limb extensors, and ankle plantar flexors. There were no differences between WBV and other types of exercise. Subgroup analyzes demonstrated that, in general, the significant results observed in the primary analyzes were not dependent on body position during vibration, kind of vibration, cumulative dose or magnitude of WBV. Conclusion: WBV was effective in increasing lower limb muscle strength. However, no significant results were observed for upper limb strength, lower limb power, and lower and upper limb muscle endurance in older adults. However, more studies are needed to better understand the physiological impacts of WBV in older.
Intestinal Obstruction for Anisakiasis: Surgical and Physical Therapy Treatment
Authors: Cózar-Bernal Fernando , Góngora-Rodríguez Jorge , Ayala-Martínez Carmen , Martín-Vega Francisco Javier , Vinolo-Gil Maria Jesus , Rodríguez-Huguet Manuel
Published: July 2023

Anisakiasis, a zoonotic disease that can lead to small intestine obstruction, has seen a significant rise in Spain. The country has become the first in Europe with an annual incidence of 8000 cases, primarily due to the popularity of consuming exotic dishes of undercooked or raw fish and the impact of climate change. The clinical presentation of anisakiasis can mimic symptoms of acute appendicitis or intestinal obstruction, leading to potential misdiagnosis. This case report describes a 37-year-old patient with no significant medical history who presented abdominal distension and intense pain in the right lower quadrant. The patient underwent surgery and received physiotherapy treatment, including therapeutic exercises and pulsed-pressure myofascial vacuum therapy, to facilitate functional recovery. The increasing incidence of anisakiasis in Spain underscores the need to consider it in the differential diagnosis of digestive diseases, given the high consumption of poorly prepared or raw fish in the region.
Three-Dimensional Characterization of Aortic Root Motion by Vascular Deformation Mapping
Authors: Kim Taeouk , Tjahjadi Nic S. , He Xuehuan , van Herwaarden JA , Patel Himanshu J. , Burris Nicholas S. , Figueroa C. Alberto
Published: July 2023

The aorta is in constant motion due to the combination of cyclic loading and unloading with its mechanical coupling to the contractile left ventricle (LV) myocardium. This aortic root motion has been proposed as a marker for aortic disease progression. Aortic root motion extraction techniques have been mostly based on 2D image analysis and have thus lacked a rigorous description of the different components of aortic root motion (e.g., axial versus in-plane). In this study, we utilized a novel technique termed vascular deformation mapping (VDM(D)) to extract 3D aortic root motion from dynamic computed tomography angiography images. Aortic root displacement (axial and in-plane), area ratio and distensibility, axial tilt, aortic rotation, and LV/Ao angles were extracted and compared for four different subject groups: non-aneurysmal, TAA, Marfan, and repair. The repair group showed smaller aortic root displacement, aortic rotation, and distensibility than the other groups. The repair group was also the only group that showed a larger relative in-plane displacement than relative axial displacement. The Marfan group showed the largest heterogeneity in aortic root displacement, distensibility, and age. The non-aneurysmal group showed a negative correlation between age and distensibility, consistent with previous studies. Our results revealed a strong positive correlation between LV/Ao angle and relative axial displacement and a strong negative correlation between LV/Ao angle and relative in-plane displacement. VDM(D)-derived 3D aortic root motion can be used in future studies to define improved boundary conditions for aortic wall stress analysis.
Interleukin 12 in the Acute Phase of the Immune Response after Excimer Laser Treatment
Authors: Resan Mirko , Cvejic Zeljka , Pancevski Igor , Thumann Gabriele , Kropp Martina , Guber Ivo , Ristic Dragana , Vojvodic Danilo , Pajic Bojan
Published: July 2023

Background and objectives: The aim of the research was to investigate the differences in the concentrations of IL-12, IL-4, IL-10, and IFN-γ in tears after LASIK and PRK procedures. Materials and methods: The study included 68 myopic eyes up to −3.0 D refractive spherical equivalent, divided into two groups: Group 1 LASIK (n = 31) and Group 2 PRK (n = 37). Three tear samples were taken from each eye: immediately before the procedure (t0), 1 h after the procedure (t1), and 24 h after the procedure (t2). The concentrations of IL-12p70, IL-4, IL-10, and IFN-γ in the tear samples were determined by flow cytometry. Participants were not taking anti-inflammatory therapy 24 h after the procedure. Results: IL-4 levels 1 h after treatment did not differ between LASIK and PRK (p = 0.990), while 24 h after PRK there was a significant decrease in IL-4 levels (p < 0.05), but not after LASIK (p = 0.476). In both the LASIK (p < 0.05) and PRK (p < 0.05) groups, there is an increase in IL-10 concentrations 1 h after treatment, which persists 24 h after LASIK (p < 0.05) but not after PRK (p = 0.081). There is an increase in IL-12p70 concentration 1 h after treatment in both the LASIK (p < 0.001) and PRK groups (p < 0.001). There is also an increase in IL-12p70 concentration 24 h after PRK (p < 0.005), but not after LASIK (p = 0.775). Conclusions: IL-4 concentration shows a significantly higher value in the LASIK group than in the PRK group after 24 h. IL-10 and IL-12p70 levels increase one hour after surgery in both groups. After 24 h, the IL-10 levels remain elevated in the LASIK group, and the IL-12p70 levels remain elevated in the PRK group. Thus, LASIK and PRK procedures show different inflammatory dynamics.
Molecular and Cellular Characterization of Avian Reticulate Scales Implies the Evo–Devo Novelty of Skin Appendages in Foot Sole
Authors: Liu Tzu-Yu , Hughes Michael W. , Wang Hao-Ven , Yang Wei-Cheng , Chuong Cheng-Ming , Wu Ping
Published: July 2023

Among amniotic skin appendages, avian feathers and mammalian hairs protect their stem cells in specialized niches, located in the collar bulge and hair bulge, respectively. In chickens and alligators, label retaining cells (LRCs), which are putative stem cells, are distributed in the hinge regions of both avian scutate scales and reptilian overlapping scales. These LRCs take part in scale regeneration. However, it is unknown whether other types of scales, for example, symmetrically shaped reticulate scales, have a similar way of preserving their stem cells. In particular, the foot sole represents a special interface between animal feet and external environments, with heavy mechanical loading. This is different from scutate-scale-covered metatarsal feet that function as protection. Avian reticulate scales on foot soles display specialized characteristics in development. They do not have a placode stage and lack β-keratin expression. Here, we explore the molecular and cellular characteristics of avian reticulate scales. RNAscope analysis reveals different molecular profiles during surface and hinge determination compared with scutate scales. Furthermore, reticulate scales express Keratin 15 (K15) sporadically in both surface- and hinge-region basal layer cells, and LRCs are not localized. Upon wounding, the reticulate scale region undergoes repair but does not regenerate. Our results suggest that successful skin appendage regeneration requires localized stem cell niches to guide regeneration.
A Hypertonic Seawater Nasal Irrigation Solution Containing Algal and Herbal Natural Ingredients Reduces Viral Load and SARS-CoV-2 Detection Time in the Nasal Cavity
Authors: Pantazopoulos Ioannis , Chalkias Athanasios , Miziou Angeliki , Spanos Michalis , Gerovasileiou Efrosyni , Rouka Erasmia , Gourgoulianis Konstantinos
Published: July 2023

Nasal irrigation is thought to decrease the viral load present in the nasal cavity. Our aim was to assess the effect of a hypertonic seawater solution [with algal and herbal natural ingredients (Sinomarin®)] on the viral load of nasopharynx in patients hospitalized with severe COVID-19 pneumonia. We conducted a prospective, randomized, controlled trial from June 2022 to December 2022. We allocated 56 patients with COVID-19 pneumonia into two groups (28 in each group)—the hypertonic seawater group [nasal irrigations with a hypertonic seawater solution (Sinomarin®) every 4 h for 16 h per day, for two consecutive days] and the control group (no nasal irrigations). A second nasopharyngeal swab was collected 48 h after the baseline nasopharyngeal swab (8 h after the last wash in the hypertonic seawater group) to estimate the SARS-CoV-2 viral load as determined by cycle threshold (Ct) values. In the hypertonic seawater group, the mean Ct values significantly increased two days after the initial measurement [ΔCt 48−0 h = 3.86 ± 3.03 cycles, p < 0.001 (95%CI: 2.69 to 5.04)]. No significant differences in the Ct values were observed in the control group [ΔCt 48−0 h = −0.14 ± 4.29, p = 0.866 (95%CI: −1.80 to −1.52)]. At follow-up, 17 patients from the hypertonic seawater group had negative test results compared to only 9 patients from the control group (p = 0.03). Nasal irrigations with a hypertonic seawater solution containing algal and herbal natural ingredients significantly decreased nasopharyngeal viral load and the detection time of SARS-CoV-2 in the nasal cavity.
Differences between Kidney Transplant Recipients from Deceased Donors with Diabetes Mellitus as Identified by Machine Learning Consensus Clustering
Authors: Thongprayoon Charat , Miao Jing , Jadlowiec Caroline C. , Mao Shennen A. , Mao Michael A. , Leeaphorn Napat , Kaewput Wisit , Pattharanitima Pattharawin , Tangpanithandee Supawit , Krisanapan Pajaree , Nissaisorakarn Pitchaphon , Cooper Matthew , Cheungpasitporn Wisit
Published: July 2023

Clinical outcomes of deceased donor kidney transplants coming from diabetic donors currently remain inconsistent, possibly due to high heterogeneities in this population. Our study aimed to cluster recipients of diabetic deceased donor kidney transplants using an unsupervised machine learning approach in order to identify subgroups with high risk of inferior outcomes and potential variables associated with these outcomes. Consensus cluster analysis was performed based on recipient-, donor-, and transplant-related characteristics in 7876 recipients of diabetic deceased donor kidney transplants from 2010 to 2019 in the OPTN/UNOS database. We determined the important characteristics of each assigned cluster and compared the post-transplant outcomes between the clusters. Consensus cluster analysis identified three clinically distinct clusters. Recipients in cluster 1 (n = 2903) were characterized by oldest age (64 ± 8 years), highest rate of comorbid diabetes mellitus (55%). They were more likely to receive kidney allografts from donors that were older (58 ± 6.3 years), had hypertension (89%), met expanded criteria donor (ECD) status (78%), had a high rate of cerebrovascular death (63%), and carried a high kidney donor profile index (KDPI). Recipients in cluster 2 (n = 687) were younger (49 ± 13 years) and all were re-transplant patients with higher panel reactive antibodies (PRA) (88 [IQR 46, 98]) who received kidneys from younger (44 ± 11 years), non-ECD deceased donors (88%) with low numbers of HLA mismatch (4 [IQR 2, 5]). The cluster 3 cohort was characterized by first-time kidney transplant recipients (100%) who received kidney allografts from younger (42 ± 11 years), non-ECD deceased donors (98%). Compared to cluster 3, cluster 1 had higher incidence of primary non-function, delayed graft function, patient death and death-censored graft failure, whereas cluster 2 had higher incidence of delayed graft function and death-censored graft failure but comparable primary non-function and patient death. An unsupervised machine learning approach characterized diabetic donor kidney transplant patients into three clinically distinct clusters with differing outcomes. Our data highlight opportunities to improve utilization of high KDPI kidneys coming from diabetic donors in recipients with survival-limiting comorbidities such as those observed in cluster 1.
Evolving Trends and Influencing Factors of the Rural Green Development Level in Chongqing
Authors: Zhu Kangwen , Song Dan , Zhang Lanxin , He Yong , Zhang Sheng , Liu Yaqun , Tian Xiaosong
Published: July 2023

Rural green development is a concrete practice of rural revitalization. Currently, research on quantitative evaluation methods for rural green development levels are not well developed. In this study, an evaluation model of the rural green development level in Chongqing City, China was developed based on the parameters of ecology, living, and production. An entropy weight method, Theil index, optimal scale regression model, and GIS were used to analyze the spatio-temporal characteristics, trends, and influencing factors of the rural green development level from 2018 to 2020 in Chongqing City. The results showed that: (1) the overall “ecology, living, and production” dimensions and the comprehensive index of the development level in the city were generally increasing, and the proportion of counties at a high-level increased from 23.68% in 2018 to 81.58% in 2020; (2) the Theil index of the city in was 0.0185, 0.0121, and 0.0114 in 2018, 2019, and 2020 respectively, indicating that the differences in development level among regions decreased as the development level increased; (3) the level of rural green development showed a clear upwards trend, and the proportion of counties with low-speed growth, medium-speed growth, and high-speed growth from 2018 to 2020 was 5.26%, 81.58%, and 13.16%, respectively; and (4) the optimal scale regression analysis showed that the factors with greater impacts on the rural green development level are social security and employment expenditure level of government finance, health expenditure level of government finance, with their contributions is 40.3% and 26%, respectively. The results from this study demonstrate the significance of exploring research methods for rural green development and ways to improve the level of rural green development.
How Did the Land Contract Disputes Evolve? Evidence from the Yangtze River Economic Belt, China
Authors: Tan Shukui , Tong Bin , Zhang Junwen
Published: July 2023

Land contracting is an important system in China. As we know, farmers and agricultural organizations acquire land management rights from collective economic organizations to carry out agricultural production. Over the past few decades, it has proved to make a huge contribution to food security and agricultural development in China. However, as land values increased, landowners, contractors, and operators were increasingly in competition over land interests and, as a result, the number of land contract disputes has rapidly increased. Land contract disputes are not only involved in social and economic issues but also related to government management and grassroots governance. Studying the temporal and spatial changes of disputes is the premise to deal with this subject. Based on the data of China Judgment Online from 2016 to 2021, this paper used descriptive statistical methods, spatial analysis tools, and Markov Chains to reveal the temporal evolution characteristics, spatial distribution trends, and grade transfer tendency of land contract disputes in the Yangtze River Economic Belt (YEB). The results showed the following: (1) From 2016 to 2021, the number of land contract disputes in the YEB increased sharply and then decreased gradually; (2) In terms of spatial distribution, land contract disputes were significantly clustered, and the level of clustering has increased in volatility. Meanwhile, the agglomeration area has continuously transferred; (3) There existed the “club convergence effect” and “spatial spillover effect” in the process of dispute grade transfer, but the overall trend was to change for the better. This study attempted to comprehensively describe the changes in land contract disputes in the YEB, and the results would serve as a useful reference for relevant regions to explore the differentiated paths to deal with land contract disputes.
The Effects of Land Reallocation on Irrigation Collective Action: Moderating Effects of Informal Organizations and Leadership
Authors: Cao Danqiu , Wang Yahua , Zang Liangzhen
Published: July 2023

The prevailing literature has extensively explored the economic ramifications of land reallocation; however, its impact on commons governance remains underexamined. This study, using the agricultural irrigation system in rural China as an example, empirically investigated how land reallocation affects collective action. Survey information from 6298 households, spread across 461 villages in 20 Chinese provinces, served as the basis for the analysis. Our findings suggest that land reallocation is unfavorable for irrigation collective action, but the presence of informal organizations and effective leadership within the village can help ameliorate these negative effects. This research contributes to a more comprehensive understanding of the nexus between land reallocation and irrigation collective action, offering potentially valuable policy insights for countries or regions worldwide that grapple with land reallocation issues.
The Early Initiation Advantages of Physical Therapy in Multiple Sclerosis—A Pilot Study
Authors: Marcu Florin Mihai , Brihan Ilarie , Ciubara Anamaria , Lupu Vasile Valeriu , Negrut Nicoleta , Jurcau Anamaria , Lupu Ancuta , Burlea Stefan Lucian , Dragan Felicia , Butnariu Lacramioara Ionela , Ignat Alina Elena , Ciubara Alexandru Bogdan
Published: July 2023

(1) Background: Multiple sclerosis (MS), a chronic progressive neurological disorder which affects the central nervous system (CNS), can result in disorders of all the functions controlled by the CNS: motor, sensory, cognitive and emotional. Physical therapy (PT), conducted through proprioceptive neuromuscular facilitation (PNF) techniques, can be customized to the individual patient’s needs and has the potential to improve the patient’s evolution. This study aims to establish if PT based on PNF techniques has a beneficial role in MS treatment. (2) Methods: We performed a prospective study on 40 patients who were diagnosed with MS and previously treated only with MS drug treatment (DT). These patients have participated in a PT program throughout one year. At the beginning and at the end of our study, after one year, we have assessed the following parameters: timed walk for 25 feet (Timed 25-Foot Walk test- T25FW test), dexterity of the upper limbs (9-Hole Peg Test—9HPT), disability level (Expanded Disability Status Scale—EDSS) and cognitive function (Paced Auditory Serial Addition Test—PASAT. (3) Results: In subjects in the early stages of MS, lower limb mobility improved significantly, T25FW decreasing from 6.46 to 5.80 (p < 0.001) and upper limb ability increased significantly in the dominant hand, 9HPT decreasing from 17.73 to 16.97 (p = 0.006) and not significantly in the non-dominant hand, 9HPT decreasing from 17.73 to 17.50 (p = 0.255). Furthermore, among these subjects, cognitive performance improved; their PASAT increased from 52.14 to 54.14 (p = 0.036), while the level of disability of these subjects improved only slightly, the EDSS scale evolving from 3.08 to 2.91 (p = 0.650). (4) Conclusions: In patients with early forms of MS, combining DT with a PT program based on PNF techniques results in: regaining muscle strength in the lower limbs, improving coordination while walking, correcting dexterity in the upper limbs and increasing the ability to concentrate.
The Effect of Environmental Disasters on Endocrine Status, Hematology Parameters, Body Composition, and Physical Performance in Young Soccer Players: A Case Study of the Aral Sea Region
Authors: Erkudov Valerii O. , Rozumbetov Kenjabek U. , González-Fernández Francisco Tomás , Pugovkin Andrey P. , Nazhimov Ilal I. , Matchanov Azat T. , Ceylan Halil İbrahim
Published: July 2023

The Aral Sea region (Uzbekistan) is infamous because of the ecological disaster characterized by the disappearance of the Aral Sea due to excessive uncontrolled water intake for agriculture needs. A new desert occurrence, soil and climate aridization led to pesticide and toxic metals environment pollution. The impact of environmental conditions in some areas of Uzbekistan on the health of soccer players is not as noticeable as, for example, the effectiveness of training, so it is not widely discussed in scientific papers. The aim of the present study was to study the features of endocrine status, hematology parameters (e.g., red blood cells (RBC) and hemoglobin (Hb)), and their influence on body composition and physical fitness performance in local young soccer players of the Aral Sea region as the territory of ecological disaster. The study involved 60 male soccer players aged from 18 to 22 years. Participants were divided into two groups: the experimental group (EG), which consisted of 30 soccer players living on the territory of the Aral ecological disaster region, and the control group (CG), which included 30 soccer players, natives of the ecologically favorable region of Uzbekistan. All volunteers had anthropometric measurements, concentrations of insulin-like growth factor-1 (IGF-1), total testosterone (TT), estradiol (E2), cortisol (C), RBC, and Hb count. Moreover, Yo-Yo Intermittent Recovery Test Level 1 (YYIRT1) and professional skills tests such as dribbling shuttle test (DSt) and goal accuracy test (GAt) were assessed. When comparing the CG group to the EG group, it was observed that the EG group exhibited statistically significantly reduced levels of TT and E2 (p < 0.05). No significant statistical difference was observed between the two groups in terms of IGF-1 and C (p > 0.05). Regarding hematological parameters, Hb, Ht, and mean corpuscular volume (MCV) were found to be significantly lower in the EG compared with the CG (p < 0.05). Moreover, the distance covered in the YYIR1 test was found to be significantly lower in soccer players within the EG compared with the CG (p < 0.05). Additionally, it was determined that there was no significant difference between the groups in terms of DSt and GAt values (p > 0.05). Lastly, significant differences were observed between the EG and CG in terms of anthropometric characteristics (diameters, skinfold, and somatotype profile) (p < 0.05). The present study showed that the changes in evaluated characteristics might result from the complex influence of endocrine-disrupting chemicals, the content of which is high in the environment of the Aral Sea region. The results obtained may help monitor the health of athletes living in an environmentally unfriendly environment.