bluuebunny/crossref_metadata_2025 huggingface dataset:
Nanotechnology-Enabled COVID-19 mRNA Vaccines
Authors: Gao Yu , Yang Kaiyun , Shelling Andrew N. , Wu Zimei
Published: August 2021

COVID-19 mRNA vaccines contain synthetic mRNA sequences encoded for the Spike proteins expressed on the surface of SARS-CoV-2, and utilize the host cells to produce specific antigens that stimulate both humoral and cellular immunities. Lipid nanoparticles are essential to facilitate the intracellular delivery of the mRNA to its action site, the ribosome, to fully exert its effect.
Regeneration Dynamics on Treefall Mounds and Pits for 10 Years after a Windfall in a Natural Mixed Forest
Authors: Yoshida Toshiya
Published: August 2021

The treefall mounds and pits resulting from uprooting caused by strong winds is an indispensable microtopography for the regeneration of many tree species through improved light conditions and mineral soil exposure. These microtopographies are expected to become more important because global warming is predicted to increase windstorm frequency. This study aimed to clarify the characteristics of mounds and pits that contribute to the early establishment of major tree species in a natural mixed forest of northern Japan. The 39 tip-up mounds caused simultaneously by a strong typhoon in September 2004 were selected. In 2006, light intensity and soil moisture contents were measured in each mound and pit. Seedlings of all tree species were counted, and in 2014, the height of saplings was measured. The initial seedling density, regardless of tree species, was significantly higher in the pits than on the mound, but the density at the 10-th growing season decreased significantly in the pits and was comparable between the two locations. Intense light conditions favor regeneration of Betula sp. (Betula platyphylla and B. ermanii). In contrast, for Abies sachalinensis and Picea glehnii, the light intensity had a negative effect, so it was concluded that regeneration of conifers would be limited under the condition where strong winds form large gaps.
Spatially Explicit Kirtland’s Warbler Habitat Management Scheduling in Michigan’s Upper Peninsula
Authors: Henderson Eric , Hoganson Howard
Published: August 2021

A spatially explicit management strategy is presented for Kirtland’s Warbler (Setophaga kirtlandii) habitat on the Hiawatha National Forest in Michigan’s Upper Peninsula. The Hiawatha National Forest has a goal of continuously providing large patches of dense young jack pine for Kirtland’s warbler breeding habitat. The problem is challenging as patches of suitable habitat are relatively short lived, forcing large shifts in the location of large patches in the future. In this study, alternative management strategies for providing habitat are described, explicitly mapped, and compared on a 70,600 ha landscape in the context of implementing many desired conditions of the forest’s land management plan. Strategies are developed by using two interacting scheduling models. Comparisons address overall habitat levels, habitat spatial arrangement through time, and financial trade-offs. The financial cost of managing habitat is high and there are further financial trade-offs associated with aggregating habitat into large patches. Furthermore, the marginal cost of habitat increases as more habitat is added to the management system. Managers may use information about the added costs of spatially explicit habitat management to help evaluate the added benefits to the species. It is often expensive to establish wildlife habitat and desirable ecological conditions, but results show that there are potential benefits from using detailed computer-aided management scheduling tools to support the decision-making process.
Sensory and Compositional Properties Affecting the Likeability of Commercially Available Australian Honeys
Authors: Hunter Maddison , Kellett Jane , Toohey Kellie , Naumovski Nenad
Published: August 2021

Honey’s composition and appearance is largely influenced by floral and geographic origins. Australian honeys are frequently sourced from supermarkets; however, properties associated with consumer preference and likeability remain relatively unknown. The aim of this study was to complete sensory and compositional analyses on a selection of commercially available Australian honeys. Samples (n = 32) were analysed for visual, olfactory and taste characteristics, with overall likeability assessed by the trained sensory panel (n = 24; M = 12). Compositional analysis included colour intensity (mAU); phenolic content; antioxidant characteristics (DPPH, CUPRAC); and physicochemical properties (pH, viscosity, total soluble solids). There were 23 honey samples that were significantly less liked when compared to the most liked honey (p < 0.05). The likeability of honey was positively associated with perceived sweetness (p < 0.01), and it was negatively associated with crystallisation; odour intensity; waxy, chemical, and fermented smell; mouthfeel; aftertaste; sourness; bitterness and pH (All p’s < 0.05). The price (AUD/100 g) was not associated with likeability (p = 0.143), suggesting price value potentially does not influence consumer preferences. Conclusively, differences in likeability between the honey samples demonstrate that consumer perception of sampled honeys is diverse. Honey preference is primarily driven by the organoleptic properties, particularly perceived negative tastes, rather than their antioxidant capacity or phenolic content.
Antimicrobial Resistance of Enterococcus sp. Isolated from Sheep and Goat Cheeses
Authors: Výrostková Jana , Regecová Ivana , Dudriková Eva , Marcinčák Slavomír , Vargová Mária , Kováčová Mariana , Maľová Jana
Published: August 2021

This study aimed to calculate the proportion of antibiotic resistance profiles of Enterococcus faecium, E. faecalis, and E. durans isolated from traditional sheep and goat cheeses obtained from a selected border area of Slovakia with Hungary (region Slanské vrchy). A total of 110 Enterococcus sp. were isolated from cheese samples, of which 52 strains (E. faecium (12), E. faecalis (28), E. durans (12)) were represented. After isolation and identification by polymerase chain reaction and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry, the enterococci (E. faecium, E. faecalis, and E. durans) were submitted to susceptibility tests against nine antimicrobial agents. In general, strains of E. faecalis were more resistant than E. durans and E. faecium. A high percentage of resistance was noted in E. faecalis to rifampicin (100%), vancomycin (85.7%), teicoplanin (71.4%), erythromycin (71.4%), minocycline (57.1%), nitrofurantoin (57.1%), ciprofloxacin (14.3%), and levofloxacin (14.3%). E. durans showed resistance to rifampicin (100%), teicoplanin (100%), vancomycin (66.7%), erythromycin (66.7%), nitrofurantoin (66.7%), and minocycline (33.3%), and E. faecium showed resistance to vancomycin, teicoplanin, and erythromycin (100%). Multidrug-resistant strains were confirmed in 80% of the 52 strains in this study. Continuous identification of Enterococcus sp. and monitoring of their incidence and emerging antibiotic resistance is important in order to prevent a potential risk to public health caused by the contamination of milk and other dairy products, such as cheeses, made on farm level.
Dried Figs Quality Improvement and Process Energy Savings by Combinatory Application of Osmotic Pretreatment and Conventional Air Drying
Authors: Andreou Varvara , Thanou Ioanna , Giannoglou Marianna , Giannakourou Maria C. , Katsaros George
Published: August 2021

This study concerns the implementation of osmotic dehydration (OD) as a pre-treatment of air-drying in fig halves, aiming at drying acceleration, energy savings and product quality improvement. The effect of solid/liquid mass ratio, process temperature (25–45 °C) and duration (up to 300 min) on water activity (aw) and transport phenomena during OD, was modelled. The effective diffusion coefficients, drying time and energy consumption, were also calculated during air-drying at 50–70 °C. At optimum OD conditions (90 min, 45 °C), the highest water loss and solid gain ratio were achieved, while the aw (equal to an initial value 0.986) was decreased to 0.929. Air-drying time of OD- and control samples was estimated at 12 and 21 h, at 60 °C, respectively, decreasing the required energy by up to 31.1%. Quality of dried figs was systematically monitored during storage. OD-assisted air-drying led to a product of improved quality and extended shelf-life.
Consumer Adoption of Plug-In Electric Vehicles in Selected Countries
Authors: Graham John D. , Brungard Eva
Published: August 2021

The transition to plug-in electric vehicles is examined from the consumer’s perspective. Since risk-averse consumers perceive disadvantages as well as advantages, consumers are reluctant to choose electric propulsion without significant nudges from the government. Norway, California, Germany and China are analyzed to determine why and how electric vehicles are promoted by public policies. Each jurisdiction has accomplished rates of electric-vehicle penetration that are far above the global average. This success is largely attributed to various policies which range from vehicle mandates, producer and/or consumer subsidies, or taxation in respective regions—otherwise PEVs remain relatively unappealing to risk-averse consumers. Demand and supply side policies have been effective tools in spurring adoption of the new electric propulsion system. Norway is one notable jurisdiction that has PEV penetration exceeding 80% of new vehicle sales despite no supply side incentives. Germany has recently surpassed California and China in PEV penetration rate, though all three jurisdictions exceeded 10 percent by 2020 or early 2021. Research is recommended to identify ways to encourage consumer adoption of electric vehicles.
Hearing Function: Identification of New Candidate Genes Further Explaining the Complexity of This Sensory Ability
Authors: Concas Maria Pina , Morgan Anna , Serra Fabrizio , Nagtegaal Andries Paul , Oosterloo Berthe C. , Seshadri Sudha , Heard-Costa Nancy , Van Camp Guy , Fransen Erik , Francescatto Margherita , Logroscino Giancarlo , Sardone Rodolfo , Quaranta Nicola , Gasparini Paolo , Girotto Giorgia
Published: August 2021

To date, the knowledge of the genetic determinants behind the modulation of hearing ability is relatively limited. To investigate this trait, we performed Genome-Wide Association Study (GWAS) meta-analysis using genotype and audiometric data (hearing thresholds at 0.25, 0.5, 1, 2, 4, and 8 kHz, and pure-tone averages of thresholds at low, medium, and high frequencies) collected in nine cohorts from Europe, South-Eastern USA, Caucasus, and Central Asia, for an overall number of ~9000 subjects. Three hundred seventy-five genes across all nine analyses were tagged by single nucleotide polymorphisms (SNPs) reaching a suggestive p-value (p < 10−5). Amongst these, 15 were successfully replicated using a gene-based approach in the independent Italian Salus in the Apulia cohort (n = 1774) at the nominal significance threshold (p < 0.05). In addition, the expression level of the replicated genes was assessed in published human and mouse inner ear datasets. Considering expression patterns in humans and mice, eleven genes were considered particularly promising candidates for the hearing function: BNIP3L, ELP5, MAP3K20, MATN2, MTMR7, MYO1E, PCNT, R3HDM1, SLC9A9, TGFB2, and YTHDC2. These findings represent a further contribution to our understanding of the genetic basis of hearing function and its related diseases.
Guardians of the Genome: BRCA2 and Its Partners
Authors: Le Hang Phuong , Heyer Wolf-Dietrich , Liu Jie
Published: August 2021

The tumor suppressor BRCA2 functions as a central caretaker of genome stability, and individuals who carry BRCA2 mutations are predisposed to breast, ovarian, and other cancers. Recent research advanced our mechanistic understanding of BRCA2 and its various interaction partners in DNA repair, DNA replication support, and DNA double-strand break repair pathway choice. In this review, we discuss the biochemical and structural properties of BRCA2 and examine how these fundamental properties contribute to DNA repair and replication fork stabilization in living cells. We highlight selected BRCA2 binding partners and discuss their role in BRCA2-mediated homologous recombination and fork protection. Improved mechanistic understanding of how BRCA2 functions in genome stability maintenance can enable experimental evidence-based evaluation of pathogenic BRCA2 mutations and BRCA2 pseudo-revertants to support targeted therapy.
Compounding Parenteral Products in Pediatric Wards—Effect of Environment and Aseptic Technique on Product Sterility
Authors: Virtanen Sonja , Kapp Karmen , Rautamo Maria , Schepel Lotta , Lindén-Lahti Carita , Cruz Cristina D. , Tammela Päivi
Published: August 2021

Parenteral products must be compounded using an aseptic technique to ensure sterility of the medicine. We compared the effect of three clinical environments as compounding areas as well as different aseptic techniques on the sterility of the compounded parenteral product. Clinical pharmacists and pediatric nurses compounded 220 samples in total in three clinical environments: a patient room, a medicine room and biological safety cabinet. The study combined four methods: observation, environmental monitoring (settle plates), monitoring of personnel (finger dab plates) and sterility testing (membrane filtration). Of the compounded samples, 99% were sterile and no significant differences emerged between the clinical environments. Based on the settle plates, the biological safety cabinet was the only area that fulfilled the requirements for eliminating microbial contamination. Most of the steps on the observation form for aseptic techniques were followed. All participants disinfected their hands, wore gloves and disinfected the septum of the vial. Non-contaminated finger dab plates were mostly detected after compounding in the biological safety cabinet. Aseptic techniques were followed relatively well in all environments. However, these results emphasize the importance of good aseptic techniques and support the recommendation of compounding parenteral products in biological safety cabinets in clinical environments.
Impacts of the COVID-19 Pandemic on Women and Early Career Archaeologists
Authors: Hoggarth Julie A. , Batty Sylvia , Bondura Valerie , Creamer Emma , Ebert Claire E. , Green-Mink Kirsten , Kieffer C. L. , Miller Heidi , Ngonadi C. V. , Pilaar Birch Suzanne E. , Pritchard Christy , Vacca Kirsten , Watkins Tia B. , Zavodny Emily , Ventresca Miller Alicia R.
Published: August 2021

The COVID-19 pandemic has had far-reaching impacts in all segments of life worldwide. While a variety of surveys have assessed the impacts of the pandemic in other fields, few studies have focused on understanding the short- and long-term impacts of the pandemic for archaeology. To assess these trends, we asked survey respondents (n = 570) if they experienced job loss and to rate the percentage of change in their economic situation, workload, teaching or research activities, and personal responsibilities. Results show alarming trends, with nearly half of those who experienced job loss being under the age of 35 and women and early career archaeologists suffering major economic losses. Impacts to workload, teaching activities, and research activities were also felt across these groups. Substantial increases in personal responsibilities (childcare, eldercare, caring for sick family members) were also identified, especially for women with children under 18 years of age. While structural inequalities have already been identified across different sectors of archaeology, the results of this survey suggest the most vulnerable populations are those most heavily affected. We recommend a variety of strategies for employers, professional organizations, funding agencies, and publishers to consider in mitigating the consequences of COVID-19, especially for women and early career scholars.
Foliar Application of ZnSO4 and CuSO4 Affects the Growth, Productivity, and Fruit Quality of Washington Navel Orange Trees (Citrus sinensis L.) Osbeck
Authors: El-Gioushy Sherif Fathy , Sami Rokayya , Al-Mushhin Amina A. M. , Abou El-Ghit Hanan M. , Gawish Mohamed S. , Ismail Khadiga Ahmed , Zewail Reda M. Y.
Published: August 2021

The goal of this study was to examine how to improve the vegetative growth, nutritional status, productivity, and fruit quality of Washington navel orange trees by examining the effect of foliar application of ZnSO4 (0, 300, and 600 mg/L) solutions in combination with CuSO4 (0, 200, and 400 mg/L) solutions on Washington navel orange trees, which were 11 years old and grown in clay loam soil with a surface irrigation system. The results showed that all the investigated measurements responded specifically to each investigated factor. ZnSO4 elicited a stronger and more effective response than CuSO4. Nonetheless, the response varied only slightly or moderately from one measurement to the next. In terms of the interaction effect between ZnSO4 and CuSO4 concentrations, the effect of each investigated factor was directly reflected in its combinations, with ZnSO4 (600 mg/L) and CuSO4 (200 and 400 mg/L) being the most effective for the majority of the measurements under consideration. When the highest level of ZnSO4 was combined with the highest level of CuSO4, the highest values for the various vegetative growth parameters shoot length and diameter, number of leaves per shoot, leaf area, and total assimilation area per shoot were obtained. As a result, the nutritional status (the highest total leaf chlorophyll and leaf mineral contents) was significantly coupled with the treatment of 600 mg/L ZnSO4 in combination with 400 mg/L CuSO4. Moreover, the combinations of the highest ZnSO4 concentration (600 mg/L) and CuSO4 concentration (400 mg/L) exhibited the greatest statistical values of the measurements of fruiting aspects as well as fruit quality. Consequently, it can be recommended that using 600 mg/L ZnSO4 in combination with 400 mg/L CuSO4 as a foliar spray on monthly basis during the period from March to July could be safely recommended under similar environmental conditions and horticulture practices adopted in the present experiment.
Economic Impacts of Horticulture Research and Extension at MSU Coastal Research and Extension Center
Authors: Posadas Benedict C. , Knight Patricia R. , Stafne Eric T. , Coker Christine E. H. , Bachman Gary , DelPrince James , Langlois Scott A. , Blythe Eugene K.
Published: August 2021

This paper summarizes the estimates of the total changes in sales, expenses, and income of participants of the horticulture research and extension programs at the Mississippi State University—Coastal Research and Extension Center for the past five years. Major items outline the estimation procedures for the past five years. The average annual values were used in estimating the total economic impacts of added gross sales, expenses, and incomes of participants in horticulture events. The cumulative total impacts reach USD 8.7 million in sales, 76 jobs, USD 1.4 million in labor income, USD 2.4 million in value-added, and USD 0.4 million in local, state, and federal taxes. In addition, the total willingness to pay for the horticulture program by the adult participants reached USD 1.8 million. In comparison, the annual public spending on the horticulture program averaged USD 1.4 million, creating additional substantial economic impacts to the region.
Factors Influencing the Use of Health Information Exchange by Physicians—Using the National Health Insurance PharmaCloud System in Taiwan
Authors: Chuang Chiou-Hwa , Li Yi-Fan , Kuo Lu-Cheng , Yang Ming-Chin , Kao Li-Ting
Published: August 2021

This study aimed to investigate the factors influencing physicians use of the PharmaCloud system in Taiwan through Technology Continuance Theory (TCT) and to construct a TCT-based structured questionnaire to demonstrate the attitude and behavior of physicians in the Taiwanese medical system. It focused on investigating “confirmation”, “perceived usefulness”, “perceived ease of use”, “attitude”, “satisfaction”, and “continuance intention” towards the preload-based comparison and manual search in PharmaCloud by attending physicians during their outpatient clinics. Path analysis was used to analyze the cause and effect relationship between variables. This study collected 528 valid questionnaires and the results of path analysis found that factors affecting physicians’ continued use of preload-based comparison in PharmaCloud included “perceived usefulness”, “satisfaction”, and “attitude” (all p < 0.001); however, factors that influenced physicians’ continued use of manual search in PharmaCloud were only “satisfaction” and “attitude” (all p < 0.001). Additionally, the effects of “perceived usefulness” and “perceived ease of use” on “satisfaction” could only be seen in preload-based comparison in PharmaCloud. In conclusion, when physicians’ actual use of PharmaCloud met their expectations, physicians had higher levels of confirmation and better perceived usefulness, which naturally increased their satisfaction and attitude towards PharmaCloud and positively prompted them to continue using it.
The Shinyanga Patient: A Patient’s Journey through HIV Treatment Cascade in Rural Tanzania
Authors: Okere Nwanneka E , Sambu Veryeh , Ndungile Yudas , van Praag Eric , Hermans Sabine , Naniche Denise , de Wit Tobias F Rinke , Maokola Werner , Gomez Gabriela B
Published: August 2021

The 2016–2017 Tanzania HIV Impact Survey (THIS) reported the accomplishments towards the 90-90-90 global HIV targets at 61-94-87, affirming the need to focus on the first 90 (i.e., getting 90% of people living with HIV (PLHIV) tested). We conducted a patient-pathway analysis to understand the gap observed, by assessing the alignment between where PLHIV seek healthcare and where HIV services are available in the Shinyanga region, Tanzania. We used existing and publicly available data from the National AIDS Control program, national surveys, registries, and relevant national reports. Region-wide, the majority (n = 458/722, 64%) of THIS respondents accessed their last HIV test at public sector facilities. There were 65.9%, 45.1%, and 74.1% who could also access antiretroviral therapy (ART), CD4 testing, and HIV viral load testing at the location of their last HIV test, respectively. In 2019, the viral suppression rate estimated among PLHIV on ART in the Shinyanga region was 91.5%. PLHIV access HIV testing mostly in public health facilities; our research shows that synergies can be achieved to improve access to services further down the cascade in this sector. Furthermore, effective engagement with the private sector (not-for-profit and for-profit) will help to achieve the last mile toward ending the HIV epidemic.
Phenotypic Clustering in Non-Cystic Fibrosis Bronchiectasis Patients: The Role of Eosinophils in Disease Severity
Authors: Wang Xuejie , Villa Carmen , Dobarganes Yadira , Olveira Casilda , Girón Rosa , García-Clemente Marta , Máiz Luis , Sibila Oriol , Golpe Rafael , Menéndez Rosario , Rodríguez-López Juan , Prados Concepción , Martinez-García Miguel Angel , Rodriguez Juan Luis , de la Rosa David , Duran Xavier , Garcia-Ojalvo Jordi , Barreiro Esther
Published: August 2021

Whether high blood eosinophil counts may define a better phenotype in bronchiectasis patients, as shown in chronic obstructive pulmonary disease (COPD), remains to be investigated. Differential phenotypic characteristics according to eosinophil counts were assessed using a biostatistical approach in a large cohort study from the Spanish Online Bronchiectasis Registry (RIBRON). The 906 patients who met the inclusion criteria were clustered into two groups on the basis of their eosinophil levels. The potential differences according to the bronchiectasis severity index (BSI) score between two groups (Mann–Whitney U test and eosinophil count threshold: 100 cells/µL) showed the most balanced cluster sizes: above-threshold and below-threshold groups. Patients above the threshold exhibited significantly better clinical outcomes, lung function, and nutritional status, while showing lower systemic inflammation levels. The proportion of patients with mild disease was higher in the above-threshold group, while the below-threshold patients were more severe. Two distinct clinical phenotypes of stable patients with non-cystic fibrosis (CF) bronchiectasis of a wide range of disease severity were established on the basis of blood eosinophil counts using a biostatistical approach. Patients classified within the above-threshold cluster were those exhibiting a mild disease, significantly better clinical outcomes, lung function, and nutritional status while showing lower systemic inflammatory levels. These results will contribute to better characterizing bronchiectasis patients into phenotypic profiles with their clinical implications.
Will Nothing Be the Same Again?: Changes in Lifestyle during COVID-19 Pandemic and Consequences on Mental Health
Authors: Caroppo Emanuele , Mazza Marianna , Sannella Alessandra , Marano Giuseppe , Avallone Carla , Claro Angelo Emilio , Janiri Delfina , Moccia Lorenzo , Janiri Luigi , Sani Gabriele
Published: August 2021

Social isolation caused by the COVID-19 pandemic has drastically affected lifestyles: from sedentary behaviors to reduced physical activity, from disrupted sleep patterns to altered dietary habits. As a consequence, serious mental and emotional responses have been registered. There was a significant decline in physical and other meaningful activities of daily living, leisure, social activity, and education. In children, collateral effects of the pandemic include inadequate nutrition with a risk of both overweight and underweight, addiction to screens, lack of schooling, and psychosocial difficulties. Older adults are frequently unable to adapt to lockdown measures and suffer from depression and cognitive complaints. Recent studies focusing on changes in lifestyle during the Covid-19 pandemic and consequences on mental health have been identified in PubMed/Medline, Scopus, Embase, and ScienceDirect. All the available literature has been retrospectively reviewed. The results of the present narrative review suggest that mental distress caused by social isolation seems to be linked not only to personality characteristics but also to several lifestyle components (sleep disruption, altered eating habits, reduced physical activity). This review aims to explore major changes in the lifestyle and quality of life and the impact of these changes on mental health, and to inform clinicians and policymakers about elements that may reduce the negative psychological effects of the quarantine period imposed during this worldwide crisis. There is an urgent need for tailored preventive, diagnostic, and therapeutic mental health interventions for the general population and for higher risk groups.
The Effects of Dietary Education Interventions on Individuals with Type 2 Diabetes: A Systematic Review and Meta-Analysis
Authors: Kim Juri , Hur Myung-Haeng
Published: August 2021

As the incidence and prevalence of diabetes increases, intervention through dietary education is becoming more important for diabetes control. This systematic review examines the evidence for the efficacy of dietary education interventions on diabetes control. The study subjects were patients with type 2 diabetes, and the main outcome variable was glycosylated hemoglobin level (HbA1c). The target studies were randomized controlled trials. Thirty-six studies were included in the analysis, of which 33 were included in the meta-analysis. The effect size between dietary education and general interventions was −0.42 (n = 5639, MD = −0.42; 95% CI −0.53 to −0.31) and was significantly different (Z = 7.73, p < 0.001). When subgroup analyses were performed following the application periods, intervention methods, and intervention contents, the mean differences in 4–6-month application, individual education, and diet-exercise-psychosocial intervention were −0.51, (n = 2742, 95% CI −0.71 to −0.32), −0.63 (n = 627, 95% CI −1.00 to −0.26), and −0.51 (n = 3244, 95% CI −0.71 to −0.32), respectively. Dietary education interventions provided for at least 3 months were highly effective in controlling HbA1c levels. Regarding the education method, individualized education was more effective, and contact or non-contact education may be applied for this. Combining diet, exercise, and psychosocial intervention is more effective than diet education alone.
Sleep and Mood Disturbances during the COVID-19 Outbreak in an Urban Chinese Population in Hong Kong: A Longitudinal Study of the Second and Third Waves of the Outbreak
Authors: Lam Chun Sing , Yu Branda Yee-Man , Cheung Denise Shuk Ting , Cheung Teris , Lam Simon Ching , Chung Ka-Fai , Ho Fiona Yan-Yee , Yeung Wing-Fai
Published: August 2021

In response to the worsening situation of the COVID pandemic, this follow-up study aimed to assess the impact of the “third wave” of the outbreak on sleep and mood disturbances among Hong Kong citizens. A total of 339 respondents included in our last study during the second wave (4–11 August 2020) joined this survey (response rate = 51.1%). The questionnaire collected data on sleep conditions, mood, stress, and risk perception. The sleep quality and mood status were assessed using the Insomnia Severity Index (ISI), General Anxiety Disorder-7 (GAD-7), and Patient Health Questionnaire-9. The weighted prevalence of insomnia, anxiety, and depression was 33.6%, 15.3%, and 22.0%, respectively. Compared with the last survey, five out of six sleep parameters significantly worsened despite the lack of difference in the ISI score. The GAD-7 score was significantly lower. Old-aged adults were less likely to maintain good sleep quality compared with middle-aged adults (adjusted OR = 4.03, 95% CI: 1.04–15.73). Respondents without psychiatric disorder were more likely to be anxiety-free across the two time points (adjusted OR = 7.12, 95% CI: 1.33–38.03). One-third of Hong Kong people reported poor sleeping quality in the third wave of the COVID-19 outbreak. Policy-makers need to propose a contingent plan to allocate mental health resources to vulnerable subpopulations.
Peptide Sequence Mapping around Bisecting GlcNAc-Bearing N-Glycans in Mouse Brain
Authors: Ohkawa Yuki , Kizuka Yasuhiko , Takata Misaki , Nakano Miyako , Ito Emi , Mishra Sushil , Akatsuka Haruna , Harada Yoichiro , Taniguchi Naoyuki
Published: August 2021

N-glycosylation is essential for many biological processes in mammals. A variety of N-glycan structures exist, of which, the formation of bisecting N-acetylglucosamine (GlcNAc) is catalyzed by N-acetylglucosaminyltransferase-III (GnT-III, encoded by the Mgat3 gene). We previously identified various bisecting GlcNAc-modified proteins involved in Alzheimer’s disease and cancer. However, the mechanisms by which GnT-III acts on the target proteins are unknown. Here, we performed comparative glycoproteomic analyses using brain membranes of wild type (WT) and Mgat3-deficient mice. Target glycoproteins of GnT-III were enriched with E4-phytohemagglutinin (PHA) lectin, which recognizes bisecting GlcNAc, and analyzed by liquid chromatograph-mass spectrometry. We identified 32 N-glycosylation sites (Asn-Xaa-Ser/Thr, Xaa ≠ Pro) that were modified with bisecting GlcNAc. Sequence alignment of identified N-glycosylation sites that displayed bisecting GlcNAc suggested that GnT-III does not recognize a specific primary amino acid sequence. The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1. These results suggest that the action of GnT-III is partially affected by the tertiary structure of target proteins, which can accommodate bisecting GlcNAc that generates a bulky flipped-back conformation of the modified glycans.
Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (Apium graveolens L.)
Authors: Cheng Qing , Wang Peng , Li Tiantian , Liu Jinkui , Zhang Yingxue , Wang Yihao , Sun Liang , Shen Huolin
Published: August 2021

Celery (Apium graveolens L.) is an important leafy vegetable worldwide. The development of F1 hybrids in celery is highly dependent on cytoplasmic male sterility (CMS) because emasculation is difficult. In this study, we first report a celery CMS, which was found in a high-generation inbred line population of the Chinese celery “tanzhixiangqin”. Comparative analysis, following sequencing and assembly of the complete mitochondrial genome sequences for this celery CMS line and its maintainer line, revealed that there are 21 unique regions in the celery CMS line and these unique regions contain 15 ORFs. Among these ORFs, only orf768a is a chimeric gene, consisting of 1497 bp sequences of the cox1 gene and 810 bp unidentified sequences located in the unique region, and the predicted protein product of orf768a possesses 11 transmembrane domains. In summary, the results of this study indicate that orf768a is likely to be a strong candidate gene for CMS induction in celery. In addition, orf768a can be a co-segregate marker, which can be used to screen CMS in celery.
Chlorogenic Acid Enhances Doxorubicin-Mediated Cytotoxic Effect in Osteosarcoma Cells
Authors: Salzillo Alessia , Ragone Angela , Spina Annamaria , Naviglio Silvio , Sapio Luigi
Published: August 2021

Despite the recurring outbreak of resistance mechanisms and adverse reactions, doxorubicin (Doxo) still remains the standard-of-care for several cancers, including osteosarcoma (OS). As an appealing source of phytochemical compounds, naturally occurring molecules have extensively been reported to overcome Doxo limitations in preclinical models. Unlike other dietary polyphenols, only few studies recognize chlorogenic acid (CGA) as a potential partner in combination therapy, while, conversely, its anticancer evidence is steadily growing, ultimately in OS. On this basis, herein we examine the cooperating effects between CGA and Doxo in U2OS and MG-63 human OS cells. With respect to Doxo alone, the concomitant administration of CGA further decreased cell viability and growth, promoting cell death potentially via apoptosis induction. Furthermore, a longer-lasting reduction in clonogenic potential deeply supported the CGA ability to improve Doxo efficacy in those cells. Remarkably, CGA treatment ameliorated Doxo-induced cytotoxicity in H9c2 rat cardiomyocyte cells instead. Although inactivation of p44/42 MAPK was detected in response to CGA plus Doxo, PD98059-mediated p44/42 MAPK impairment enhanced the combination outcome in OS cells. These findings firstly propose CGA as a promising chemosensitizer and cardioprotective agent in OS therapy, suggesting the p44/42 MAPK pathway as relevantly involved in CGA-mediated Doxo susceptibility.
Enriched Alternative Splicing in Islets of Diabetes-Susceptible Mice
Authors: Wilhelmi Ilka , Neumann Alexander , Jähnert Markus , Ouni Meriem , Schürmann Annette
Published: August 2021

Dysfunctional islets of Langerhans are a hallmark of type 2 diabetes (T2D). We hypothesize that differences in islet gene expression alternative splicing which can contribute to altered protein function also participate in islet dysfunction. RNA sequencing (RNAseq) data from islets of obese diabetes-resistant and diabetes-susceptible mice were analyzed for alternative splicing and its putative genetic and epigenetic modulators. We focused on the expression levels of chromatin modifiers and SNPs in regulatory sequences. We identified alternative splicing events in islets of diabetes-susceptible mice amongst others in genes linked to insulin secretion, endocytosis or ubiquitin-mediated proteolysis pathways. The expression pattern of 54 histones and chromatin modifiers, which may modulate splicing, were markedly downregulated in islets of diabetic animals. Furthermore, diabetes-susceptible mice carry SNPs in RNA-binding protein motifs and in splice sites potentially responsible for alternative splicing events. They also exhibit a larger exon skipping rate, e.g., in the diabetes gene Abcc8, which might affect protein function. Expression of the neuronal splicing factor Srrm4 which mediates inclusion of microexons in mRNA transcripts was markedly lower in islets of diabetes-prone compared to diabetes-resistant mice, correlating with a preferential skipping of SRRM4 target exons. The repression of Srrm4 expression is presumably mediated via a higher expression of miR-326-3p and miR-3547-3p in islets of diabetic mice. Thus, our study suggests that an altered splicing pattern in islets of diabetes-susceptible mice may contribute to an elevated T2D risk.
Crosstalk between Light- and Temperature-Mediated Processes under Cold and Heat Stress Conditions in Plants
Authors: Janda Tibor , Prerostová Sylva , Vanková Radomíra , Darkó Éva
Published: August 2021

Extreme temperatures are among the most important stressors limiting plant growth and development. Results indicate that light substantially influences the acclimation processes to both low and high temperatures, and it may affect the level of stress injury. The interaction between light and temperature in the regulation of stress acclimation mechanisms is complex, and both light intensity and spectral composition play an important role. Higher light intensities may lead to overexcitation of the photosynthetic electron transport chain; while different wavelengths may act through different photoreceptors. These may induce various stress signalling processes, leading to regulation of stomatal movement, antioxidant and osmoregulation capacities, hormonal actions, and other stress-related pathways. In recent years, we have significantly expanded our knowledge in both light and temperature sensing and signalling. The present review provides a synthesis of results for understanding how light influences the acclimation of plants to extreme low or high temperatures, including the sensing mechanisms and molecular crosstalk processes.
Spreading and Establishment of the Non Indigenous Species Caprella scaura (Amphipoda: Caprellidae) in the Central Region of the Aegean Sea (Eastern Mediterranean Sea)
Authors: Lolas Alexios , Karapanagiotidis Ioannis T. , Panagiotaki Panagiota , Vafidis Dimitris
Published: August 2021

Caprella scaura is an invasive amphipod, native to the Indian Ocean, which has already spread to several regions of the world, including the Mediterranean Sea. The present study reports the first occurrence of the species on fish farms cages in Greece, in the Pagasitikos Gulf. Specimens were collected from colonies of the bryozoan Bugula neritina. Basic aspects of the population dynamics of the species, such as the population structure, sex ratio, and size frequency were studied for 13 months and tested for differences between two depth levels (30 cm and 5 m). Population density was significantly different between the two sampled depths. All the demographic categories were present during the whole study period, indicating that the species follows a continuous reproduction pattern in the region. Males were typically larger than females, but females were more abundant in most samples. It seems that the species is well established in the region and is probably moving towards the northern parts of the Aegean Sea.
Fungal Biodiversity in Salt Marsh Ecosystems
Authors: Calabon Mark S. , Jones E. B. Gareth , Promputtha Itthayakorn , Hyde Kevin D.
Published: August 2021

This review brings together the research efforts on salt marsh fungi, including their geographical distribution and host association. A total of 486 taxa associated with different hosts in salt marsh ecosystems are listed in this review. The taxa belong to three phyla wherein Ascomycota dominates the taxa from salt marsh ecosystems accounting for 95.27% (463 taxa). The Basidiomycota and Mucoromycota constitute 19 taxa and four taxa, respectively. Dothideomycetes has the highest number of taxa, which comprises 47.12% (229 taxa), followed by Sordariomycetes with 167 taxa (34.36%). Pleosporales is the largest order with 178 taxa recorded. Twenty-seven genera under 11 families of halophytes were reviewed for its fungal associates. Juncus roemerianus has been extensively studied for its associates with 162 documented taxa followed by Phragmites australis (137 taxa) and Spartina alterniflora (79 taxa). The highest number of salt marsh fungi have been recorded from Atlantic Ocean countries wherein the USA had the highest number of species recorded (232 taxa) followed by the UK (101 taxa), the Netherlands (74 taxa), and Argentina (51 taxa). China had the highest number of salt marsh fungi in the Pacific Ocean with 165 taxa reported, while in the Indian Ocean, India reported the highest taxa (16 taxa). Many salt marsh areas remain unexplored, especially those habitats in the Indian and Pacific Oceans areas that are hotspots of biodiversity and novel fungal taxa based on the exploration of various habitats.
Analyzing the Changes of the Meaning of Customary Land in the Context of Land Grabbing in Malawi
Authors: Bae Yuh-Jin
Published: August 2021

Ordinary Malawians who live in customary land have been suffering from land grabbing due to their weak and ill-defined land rights. Although Malawi has experienced a number of land reforms that should have contributed to strengthening customary land rights, many people in customary land still suffer from land grabbing. Accordingly, it is important to understand the factors that lead to land grabbing in customary land in Malawi. Thus, by looking at the overview of land laws and policies throughout history, this study has two aims: (1) to analyze the historical changes in the meaning and position of customary land in Malawi and (2) to analyze the land grabbers in Malawi before, during, and after the colonial era. In order to achieve the main goals, this research mainly analyzes land laws and policies connected to customary land in Malawi. The main findings of this research are that (1) the meaning of customary land changed before and after the colonial period, but little has changed between the colonial period and the present. Since the creation of land laws during the colonial period, the land rights of the people who live in customary land have not been secured, and (2) the land grabbers changed from the British colonial rulers and European settlers to the Government of Malawi. Further, with the recent land laws, such as Land Act 2016 and Customary Land Act 2016, wealthy Malawians may become new land grabbers who can afford to obtain the customary estate grants. By examining the main results, it was found that from the colonial period until the present, customary land has been vulnerable to land grabbing as its weak position still resembles that of the colonial era. Thus, Malawi appears to face significant challenges in amending its customary land laws for the benefit of the poor.
Novel Green In Situ Synthesis of ZnO Nanoparticles on Cotton Using Pomegranate Peel Extract
Authors: Verbič Anja , Šala Martin , Jerman Ivan , Gorjanc Marija
Published: August 2021

This work presents the novel and entirely green in situ synthesis of zinc oxide nanoparticles (ZnO-NP) on cotton fabric. Pomegranate peel extract was used as a reducing agent and wood ash extract was used as an alkali source for the formation of ZnO-NP from zinc acetate. Four different synthesis methods, which varied in drying between immersion of fabric in the active solutions for synthesis and the use of padding and ultrasonication, were investigated to evaluate the most suitable one to achieve excellent ultraviolet (UV) protective properties of the functionalized textile. For comparison, the cotton fabrics were also functionalized with each active solution separately or in a combination of two (i.e., Zn-acetate and plant extract). Scanning electron microscopy (SEM), inductively coupled plasma mass spectroscopy (ICP-MS), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD) analysis, and atomic force microscopy (AFM) confirm the successful formation of ZnO-NP on cotton. Among the synthesis methods, the method that included continuous drying of the samples between immersion in the active solutions for synthesis (Method 4) was found to be the most suitable to deliver uniformly impregnated cotton fibers with numerous small ZnO wurtzite structured crystals and excellent UV protection, with a UV protection factor of 154.0. This research presents an example of a green circular economy where a bio-waste material can be used to produce ZnO-NP directly on cotton at low temperatures and short treatment times without the addition of chemicals and enables the production of cellulosic fabrics with excellent UV protection.
Enhanced Electron Heat Conduction in TaS3 1D Metal Wire
Authors: Yi Hojoon , Bahng Jaeuk , Park Sehwan , Dang Dang Xuan , Sakong Wonkil , Kang Seungsu , Ahn Byung-wook , Kim Jungwon , Kim Ki Kang , Lim Jong Tae , Lim Seong Chu
Published: August 2021

The 1D wire TaS3 exhibits metallic behavior at room temperature but changes into a semiconductor below the Peierls transition temperature (Tp), near 210 K. Using the 3ω method, we measured the thermal conductivity κ of TaS3 as a function of temperature. Electrons dominate the heat conduction of a metal. The Wiedemann–Franz law states that the thermal conductivity κ of a metal is proportional to the electrical conductivity σ with a proportional coefficient of L0, known as the Lorenz number—that is, κ=σLoT. Our characterization of the thermal conductivity of metallic TaS3 reveals that, at a given temperature T, the thermal conductivity κ is much higher than the value estimated in the Wiedemann–Franz (W-F) law. The thermal conductivity of metallic TaS3 was approximately 12 times larger than predicted by W-F law, implying L=12L0. This result implies the possibility of an existing heat conduction path that the Sommerfeld theory cannot account for.
Integral Modeling for Deviation Correction Trajectory of the Mechanical Vertical Drilling System
Authors: Chai Lin , Zhang Kai , Yang Dengwen , Liu Baolin , Zhang Delong
Published: August 2021

The deviation correction trajectory of the mechanical vertical drilling system (MVDS) is very important because it is the final embodiment of performance. However, it is impossible to obtain it at the design stage, except when using simulation methods. In this paper, tool face angle model and other theoretical models were established, respectively, and the trajectory simulation method was created through model coupling. Next, the method was used to simulate the trajectory of MVDS under two typical working conditions. The results indicate that the critical deviation angle is the deviation control accuracy of the MVDS. The existence of critical deflection angle makes MVDS correct deviation and change azimuth at the same time, resulting in the trajectory being a three-dimensional curve, which has the tendency of drifting to the left. Furthermore, the deviation and azimuth change rate are constantly changing in the process of drilling. The results also show that the MVDS is unable to correct the horizontal displacement of the downhole. The proposed method and analysis results are helpful to find out and solve the problem of the current design as soon as possible, and to provide guidance for the subsequent structure optimization.