bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamic Tariff for Day-Ahead Congestion Management in Agent-Based LV Distribution Networks
Authors: Haque Niyam , Tomar Anuradha , Nguyen Phuong , Pemen Guus
Published: January 2020

Capacity challenges are becoming more frequent phenomena in residential distribution networks with new forms of loads, distributed renewable energy resources (RES) and price-responsive applications. Different types of demand response programs have been introduced to tackle these challenges through iterative changes in price and/or contractual participations based on incentives. In this research, a dynamic network tariff-based demand response program is proposed to address congestion problems in low-voltage (LV) networks. The formulation takes advantage of the scalable architecture of the agent-based systems that allows local decision making with limited communication. Energy consumption schedules are developed on a day-ahead basis depending on the expected cost of overloading for a number of probable scenarios. The performance of the proposed approach has been tested through simulations in the unbalanced IEEE European LV test feeder. Simulation results reveal up to 82% reduction in congestion on a monthly basis, while maintaining the quality of supply in the network.
The Effect of Light Wavelength on CO2 Capture, Biomass Production and Nutrient Uptake by Green Microalgae: A Step Forward on Process Integration and Optimisation
Authors: Esteves Ana F. , Soares Olívia S. G. P. , Vilar Vítor J. P. , Pires José C. M. , Gonçalves Ana L.
Published: January 2020

Microalgae have drawn the attention of several researchers as an alternative to the traditional physicochemical CO2 capture methods, since they can convert CO2 and water into organic matter and release oxygen into the atmosphere. Microalgal growth can be improved by changing light supply, such as light intensity, wavelength, and photoperiod. In this study, the effect of different light wavelengths on CO2 capture, nutrient removal from a synthetic effluent and biomass production of Chlorella vulgaris, Tetradesmus obliquus and Neochloris oleoabundans was studied. The experiments were conducted with light-emitting diodes (LEDs) with different wavelengths: 380–750 nm (white), 620–750 nm (red) and 450–495 nm (blue). The maximum specific growth rate was obtained by N. oleoabundans with white LEDs (0.264 ± 0.005 d−1), whereas the maximum biomass productivity (14 ± 4 mgdw L−1 d−1) and CO2 fixation rate (11.4 mgCO2 L−1 d−1) were obtained by C. vulgaris (also with white LEDs). Nitrogen and phosphorus removal efficiencies obtained under white light conditions were also the highest for the three studied microalgae.
Flux-Based Ozone Risk Assessment for a Plant Injury Index (PII) in Three European Cool-Temperate Deciduous Tree Species
Authors: Hoshika Yasutomo , Carrari Elisa , Mariotti Barbara , Martini Sofia , De Marco Alessandra , Sicard Pierre , Paoletti Elena
Published: January 2020

This study investigated visible foliar ozone (O3) injury in three deciduous tree species with different growth patterns (indeterminate, Alnus glutinosa (L.) Gaertn.; intermediate, Sorbus aucuparia L.; and determinate, Vaccinium myrtillus L.) from May to August 2018. Ozone effects on the timing of injury onset and a plant injury index (PII) were investigated using two O3 indices, i.e., AOT40 (accumulative O3 exposure over 40 ppb during daylight hours) and PODY (phytotoxic O3 dose above a flux threshold of Y nmol m−2 s−1). A new parameterization for PODY estimation was developed for each species. Measurements were carried out in an O3 free-air controlled exposure (FACE) experiment with three levels of O3 treatment (ambient, AA; 1.5 × AA; and 2.0 × AA). Injury onset was found in May at 2.0 × AA in all three species and the timing of the onset was determined by the amount of stomatal O3 uptake. It required 4.0 mmol m−2 POD0 and 5.5 to 9.0 ppm·h AOT40. As a result, A. glutinosa with high stomatal conductance (gs) showed the earliest emergence of O3 visible injury among the three species. After the onset, O3 visible injury expanded to the plant level as confirmed by increased PII values. In A. glutinosa with indeterminate growth pattern, a new leaf formation alleviated the expansion of O3 visible injury at the plant level. V. myrtillus showed a dramatic increase of PII from June to July due to higher sensitivity to O3 in its flowering and fruiting stage. Ozone impacts on PII were better explained by the flux-based index, PODY, as compared with the exposure-based index, AOT40. The critical levels (CLs) corresponding to PII = 5 were 8.1 mmol m−2 POD7 in A. glutinosa, 22 mmol m−2 POD0 in S. aucuparia, and 5.8 mmol m−2 POD1 in V. myrtillus. The results highlight that the CLs for PII are species-specific. Establishing species-specific O3 flux-effect relationships should be key for a quantitative O3 risk assessment.
Occurrence of European Mistletoe (Viscum album L.) on Forest Trees in Poland and Its Dynamics of Spread in the Period 2008–2018
Authors: Lech Paweł , Żółciak Anna , Hildebrand Robert
Published: January 2020

As recently reported, European mistletoe has become an important agent of damage to forest trees in Central Europe. To document the extent of its impact, host and environmental preferences, as well as spatial and temporal dynamics, we have analyzed the results of 10 years of annual assessments of tree damage in level 1 plots of country-wide national forest monitoring. Mistletoe was found on 12 forest tree species, most frequently on silver fir and Scots pine, much less often on birches and deciduous admixture species. Common beech, pedunculate, and sessile oaks, as well as coniferous admixture species, were found to be free from the parasite. Mistletoe was more common on larger trees, mainly those in the 1st and 2nd Kraft classes, characterized by a higher DBH value than trees without V. album. Infestation was also age-dependent with low numbers of infested trees in young age classes and a much higher proportion of infested trees being over 80–100 years in age. Defoliation of trees colonized by mistletoe was found to be significantly higher than in trees free from the parasite, confirming the adverse effect of infestation on tree health. The increase of relative incidence of tree infestation by mistletoe in forests in Poland was high throughout the entire period 2008–2018 and significant for the most threatened host species: silver fir, Scots pine, and birches. It was also found that spread direction was from west to east and that the occurrence of V. album shows clear regionalization. The most endangered forests were located within a 200 km wide zone ranging through lowland Central Poland from the border with Germany down to the border with Ukraine and Belarus. The highest concentration occurred in the Western part of the country. Outside this zone (to the north and south) the mistletoe was much less abundant as expressed by the amount of injuries caused by the parasite or infested trees per plot.
Current Insights into Monitoring, Bioaccumulation, and Potential Health Effects of Microplastics Present in the Food Chain
Authors: van Raamsdonk Leonard W. D. , van der Zande Meike , Koelmans Albert A. , Hoogenboom Ron L. A. P. , Peters Ruud J. B. , Groot Maria J. , Peijnenburg Ad A. C. M. , Weesepoel Yannick J. A.
Published: January 2020

Microplastics (MPs) are considered an emerging issue as environmental pollutants and a potential health threat. This review will focus on recently published data on concentrations in food, possible effects, and monitoring methods. Some data are available on concentrations in seafood (fish, bivalves, and shrimps), water, sugar, salt, and honey, but are lacking for other foods. Bottled water is a considerable source with numbers varying between 2600 and 6300 MPs per liter. Particle size distributions have revealed an abundance of particles smaller than 25 µm, which are considered to have the highest probability to pass the intestinal border and to enter the systemic circulation of mammals. Some studies with mice and zebrafish with short- or medium-term exposure (up to 42 days) have revealed diverse results with respect to both the type and extent of effects. Most notable modifications have been observed in gut microbiota, lipid metabolism, and oxidative stress. The principal elements of MP monitoring in food are sample preparation, detection, and identification. Identified data gaps include a lack of occurrence data in plant- and animal-derived food, a need for more data on possible effects of different types of microplastics, a lack of in silico models, a lack of harmonized monitoring methods, and a further development of quality assurance.
Pigment Intensity in Dogs is Associated with a Copy Number Variant Upstream of KITLG
Authors: Weich Kalie , Affolter Verena , York Daniel , Rebhun Robert , Grahn Robert , Kallenberg Angelica , Bannasch Danika
Published: January 2020

Dogs exhibit a wide variety of coat color types, and many genes have been identified that control pigment production, appearance, and distribution. Some breeds, such as the Nova Scotia Duck Tolling Retriever (NSDTR), exhibit variation in pheomelanin pigment intensity that is not explained by known genetic variants. A genome-wide association study comparing light red to dark red in the NSDTR identified a significantly associated region on canine chromosome 15 (CFA 15:23 Mb–38 Mb). Coverage analysis of whole genome sequence data from eight dogs identified a 6 kb copy number variant (CNV) 152 kb upstream of KITLG. Genotyping with digital droplet PCR (ddPCR) confirmed a significant association between an increased copy number with the dark-red coat color in NSDTR (p = 6.1 × 10−7). The copy number of the CNV was also significantly associated with coat color variation in both eumelanin and pheomelanin-based Poodles (p = 1.5 × 10−8, 4.0 × 10−9) and across other breeds. Moreover, the copy number correlated with pigment intensity along the hair shaft in both pheomelanin and eumelanin coats. KITLG plays an important role in melanogenesis, and variants upstream of KITLG have been associated with coat color variation in mice as well as hair color in humans consistent with its role in the domestic dog.
Off Earth Identification of Bacterial Populations Using 16S rDNA Nanopore Sequencing
Authors: Burton Aaron S. , Stahl Sarah E. , John Kristen K. , Jain Miten , Juul Sissel , Turner Daniel J. , Harrington Eoghan D. , Stoddart David , Paten Benedict , Akeson Mark , Castro-Wallace Sarah L.
Published: January 2020

The MinION sequencer has made in situ sequencing feasible in remote locations. Following our initial demonstration of its high performance off planet with Earth-prepared samples, we developed and tested an end-to-end, sample-to-sequencer process that could be conducted entirely aboard the International Space Station (ISS). Initial experiments demonstrated the process with a microbial mock community standard. The DNA was successfully amplified, primers were degraded, and libraries prepared and sequenced. The median percent identities for both datasets were 84%, as assessed from alignment of the mock community. The ability to correctly identify the organisms in the mock community standard was comparable for the sequencing data obtained in flight and on the ground. To validate the process on microbes collected from and cultured aboard the ISS, bacterial cells were selected from a NASA Environmental Health Systems Surface Sample Kit contact slide. The locations of bacterial colonies chosen for identification were labeled, and a small number of cells were directly added as input into the sequencing workflow. Prepared DNA was sequenced, and the data were downlinked to Earth. Return of the contact slide to the ground allowed for standard laboratory processing for bacterial identification. The identifications obtained aboard the ISS, Staphylococcus hominis and Staphylococcus capitis, matched those determined on the ground down to the species level. This marks the first ever identification of microbes entirely off Earth, and this validated process could be used for in-flight microbial identification, diagnosis of infectious disease in a crewmember, and as a research platform for investigators around the world.
Key Topics and Future Perspectives in Natural Hazards Research
Authors: Tapete Deodato
Published: January 2020

Since early 2018 the “Natural Hazards” Section of Geosciences journal has aimed to publish pure, experimental, or applied research that is focused on advancing methodologies, technologies, expertise, and capabilities to detect, characterize, monitor, and model natural hazards and assess their associated risks. This stream of geoscientific research has reached a high degree of specialization and represents a multi-disciplinary research realm. To inaugurate this section, the Special Issue “Key Topics and Future Perspectives in Natural Hazards Research” was launched. After a year and half since the call for papers was initially opened, the special issue is now completed with the editorial introducing the collection of 10 selected papers covering the following hot topics of natural hazards research: (i) trends in publications and research directions at international level; (ii) the role of Big Data in natural disaster management; (iii) assessment of seismic risk through the understanding and quantification of its three components (i.e., hazard, vulnerability and exposure/impact); (iv) climatic/hydro-meteorological hazards (i.e., drought, hurricanes); and (v) scientific analysis of past incidents and disaster forensics (i.e., the Oroville Dam 2017 spillway incident). The present editorial provides a summary of each paper of the collection within the current context of scientific research on natural hazards, pointing out the salient results and key messages.
Activated Sludge Microbial Community and Treatment Performance of Wastewater Treatment Plants in Industrial and Municipal Zones
Authors: Yang Yongkui , Wang Longfei , Xiang Feng , Zhao Lin , Qiao Zhi
Published: January 2020

Controlling wastewater pollution from centralized industrial zones is important for reducing overall water pollution. Microbial community structure and diversity can adversely affect wastewater treatment plant (WWTP) performance and stability. Therefore, we studied microbial structure, diversity, and metabolic functions in WWTPs that treat industrial or municipal wastewater. Sludge microbial community diversity and richness were the lowest for the industrial WWTPs, indicating that industrial influents inhibited bacterial growth. The sludge of industrial WWTP had low Nitrospira populations, indicating that influent composition affected nitrification and denitrification. The sludge of industrial WWTPs had high metabolic functions associated with xenobiotic and amino acid metabolism. Furthermore, bacterial richness was positively correlated with conventional pollutants (e.g., carbon, nitrogen, and phosphorus), but negatively correlated with total dissolved solids. This study was expected to provide a more comprehensive understanding of activated sludge microbial communities in full-scale industrial and municipal WWTPs.
Forecast of Dengue Cases in 20 Chinese Cities Based on the Deep Learning Method
Authors: Xu Jiucheng , Xu Keqiang , Li Zhichao , Meng Fengxia , Tu Taotian , Xu Lei , Liu Qiyong
Published: January 2020

Dengue fever (DF) is one of the most rapidly spreading diseases in the world, and accurate forecasts of dengue in a timely manner might help local government implement effective control measures. To obtain the accurate forecasting of DF cases, it is crucial to model the long-term dependency in time series data, which is difficult for a typical machine learning method. This study aimed to develop a timely accurate forecasting model of dengue based on long short-term memory (LSTM) recurrent neural networks while only considering monthly dengue cases and climate factors. The performance of LSTM models was compared with the other previously published models when predicting DF cases one month into the future. Our results showed that the LSTM model reduced the average the root mean squared error (RMSE) of the predictions by 12.99% to 24.91% and reduced the average RMSE of the predictions in the outbreak period by 15.09% to 26.82% as compared with other candidate models. The LSTM model achieved superior performance in predicting dengue cases as compared with other previously published forecasting models. Moreover, transfer learning (TL) can improve the generalization ability of the model in areas with fewer dengue incidences. The findings provide a more precise forecasting dengue model and could be used for other dengue-like infectious diseases.
Design and Validation of a Questionnaire on Communicating Bad News in Nursing: A Pilot Study
Authors: González-Cabrera Manuel , Ortega-Martínez Ana Raquel , Martínez-Galiano Juan Miguel , Hernández-Martínez Antonio , Parra-Anguita Laura , Frías-Osuna Antonio
Published: January 2020

Communicating bad news (CBN) is a fundamental skill in nursing; nevertheless, few instruments exist for its evaluation. This study presents a questionnaire designed to measure nurses’ knowledge and ability of CBN, as well as the analysis of its psychometric properties. Based on a literature search, the initial dimensions of CBN were identified to construct the questionnaires’ items, which were evaluated by experts for the validity of the items’ contents. Construct validity and reliability of the resulting questionnaire was carried out in a sample of 71 nurses of an Andalusian university hospital. A questionnaire with 25 items was constructed with a high internal consistency (Cronbach’s alpha 0.816). The content validity was evaluated via a literature review and additionally by the assessment of seven experts. The Kaiser-Meyer-Olkin test (KMO) obtained a score of 0.683, and the Bartlett test of sphericity a value of p < 0.001. The principal component analysis supported a construct of four dimensions. This questionnaire was found to be a valid and reliable instrument with a high internal consistency for the evaluation of CBN knowledge and skills of nursing professionals.
Exploring Mechanisms of Action: Using a Testing Typology to Understand Intervention Performance in an HIV Self-Testing RCT in England and Wales
Authors: Witzel T. , Weatherburn Peter , Bourne Adam , Rodger Alison , Bonell Chris , Gafos Mitzy , Trevelion Roy , Speakman Andrew , Lampe Fiona , Ward Denise , Dunn David , Gabriel Michelle , McCabe Leanne , Harbottle Justin , Collaco Moraes Yolanda , Michie Susan , Phillips Andrew , McCormack Sheena , Burns Fiona
Published: January 2020

SELPHI involves two interventions: A provides one HIV self-testing (HIVST) kit; B offers 3-monthly repeat HIVST kits if participants report ongoing risk. A logic model underpinned by the Behaviour Change Wheel informed the design of the intervention. SELPHI recruited 10,135 cis-men and trans people in England and Wales, all reporting anal sex with a man. This paper explores how the interventions were experienced and the mechanisms of action leading to impact for different groups of trial participants. In-depth interviews with 37 cis-men who have sex with men (MSM) were used to inductively categorise participants based on sexual and HIV testing histories. Themes relating to intervention experiences and impacts were mapped onto SELPHI-hypothesised intermediate outcomes to consider intervention impacts. Three groups were identified: ‘inexperienced testers’ engaged with SELPHI to overcome motivational and social and physical opportunity testing barriers. For ‘pro self-testers’, testing frequency was constrained by psychological and social barriers and lack of opportunity. ‘Opportunistic adopters’ engaged in HIVST for novelty and convenience. Perceived impacts for inexperienced testers were most closely aligned with the logic model, but for opportunistic adopters there was little evidence of impact. Distinctive groups were discernible with divergent intervention experiences. Using COM-B as a model for understanding behaviour change in relation to HIVST, our results indicate how HIVST interventions could be adapted to respond to different needs based on the target population’s demographic and behavioural features.
Predicting Voluntary Exercise Training among Korean Firefighters: Using Elicitation Study and the Theory of Planned Behavior
Authors: Lee Chung Gun , Middlestadt Susan E. , Park Seiyeong , Kwon Junhye , Noh Kyoungmin , Seo Dong-il , Song Wook , Park Jung-jun , Lee Han-joon , Kang Hyun Joo , Ahn Yeon Soon
Published: January 2020

Background: Firefighters are required to have high levels of aerobic and anaerobic power because they often perform physically demanding work in dangerous environments. Therefore, it is important to find out salient factors influencing voluntary exercise training among Korean firefighters based on well-validated theory. Methods: The present study conducted an elicitation study to elicit salient behavioral, normative, and control beliefs about exercise training among Korean firefighters and identified salient beliefs that have a significant indirect effect on behavior through intention using structural equation modeling. Results: Although ten modal salient beliefs obtained from our elicitation study are similar to those elicited from previous TPB belief-based research with a focus on exercise behavior, only three of these (i.e., “improves my physical ability” (coef. = 0.078, p = 0.006), “takes too much time” (coef. = 0.064, p = 0.023), and “colleagues” (coef. = 0.069, p = 0.016) indirectly influenced exercise training behavior through intention among Korean firefighters. Conclusions: Our results may contribute to the literature by providing important information suggesting that three modal salient beliefs are major cognitive determinants of exercise training behavior among Korean firefighters and they may play an essential role in developing effective programs or policies for promoting Korean firefighters’ exercise training.
A Data-Driven Framework for Walkability Measurement with Open Data: A Case Study of Triple Cities, New York
Authors: Deng Chengbin , Dong Xiaoyu , Wang Huihai , Lin Weiying , Wen Hao , Frazier John , Ho Hung Chak , Holmes Louisa
Published: January 2020

Walking is the most common, environment-friendly, and inexpensive type of physical activity. To perform in-depth walkability analysis, one option is to objectively evaluate different aspects of built environment related to walkability. In this study, we proposed a computational framework for walkability measurement using open data. Three major steps of this framework include the web scrapping of publicly available online data, determining varying weights of variables, and generating a synthetic walkability index. The results suggest three major conclusions. First, the proposed framework provides an explicit mechanism for walkability measurement. Second, the synthetic walkability index from this framework is comparable to Walk Score, and it tends to have a slightly higher sensitivity, especially in highly walkable areas in urban core. Third, this framework was effectively applied in a metropolitan area that contains three small cities that together represent a small, old shrinking region, which extends the topical area in the literature. This framework has the potential to quantify walkability in any city, especially cities with a small population where walkability has rarely been studied, or those having no quantification indicator. For such areas, researchers can calculate the synthetic walkability index based on this framework, to assist urban planners, community leaders, health officials, and policymakers in their practices to improve the walking environment of their communities.
Genome-Wide Identification of QTLs for Grain Protein Content Based on Genotyping-by-Resequencing and Verification of qGPC1-1 in Rice
Authors: Wu Yi-Bo , Li Guan , Zhu Yu-Jun , Cheng Yi-Chen , Yang Jin-Yu , Chen Hui-Zhe , Song Xian-Jun , Ying Jie-Zheng
Published: January 2020

To clarify the genetic mechanism underlying grain protein content (GPC) and to improve rice grain qualities, the mapping and cloning of quantitative trait loci (QTLs) controlling the natural variation of GPC are very important. Based on genotyping-by-resequencing, a total of 14 QTLs were detected with the Huanghuazhan/Jizi1560 (HHZ/JZ1560) recombinant inbred line (RIL) population in 2016 and 2017. Seven of the fourteen QTLs were repeatedly identified across two years. Using three residual heterozygote-derived populations, a stably inherited QTL named as qGPC1-1 was validated and delimited to a ~862 kb marker interval JD1006–JD1075 on the short arm of chromosome 1. Comparing the GPC values of the RIL population determined by near infrared reflectance spectroscopy (NIRS) and Kjeldahl nitrogen determination (KND) methods, high correlation coefficients (0.966 and 0.983) were observed in 2016 and 2017. Furthermore, 12 of the 14 QTLs were identically identified with the GPC measured by the two methods. These results indicated that instead of the traditional KND method, the rapid and easy-to-operate NIRS was suitable for analyzing a massive number of samples in mapping and cloning QTLs for GPC. Using the gel-based low-density map consisted of 208 simple sequence repeat (SSR) and insert/deletion (InDel) markers, the same number of QTLs (fourteen) were identified in the same HHZ/JZ1560 RIL population, and three QTLs were repeatedly detected across two years. More stably expressed QTLs were identified based on the genome resequencing, which might be attributed to the high-density map, increasing the detection power of minor QTLs. Our results are helpful in dissecting the genetic basis of GPC and improving rice grain qualities through molecular assisted selection.
25-Hydroxycholesterol Inhibits Adipogenic Differentiation of C3H10T1/2 Pluripotent Stromal Cells
Authors: Moseti Dorothy , Regassa Alemu , Chen Chongxiao , O Karmin , Kim Woo Kyun
Published: January 2020

Understanding of adipogenesis is important to find remedies for obesity and related disorders. In addition, it is also critical in bone disorders because there is a reciprocal relationship between adipogenesis and osteogenesis in bone micro-environment. Oxysterols are pro-osteogenic and anti-adipogenic molecules via hedgehog activation in pluripotent bone marrow stomal cells. However, no study has evaluated the role of specific oxysterols in C3H10T1/2 cells, which are a good cell model for studying osteogenesis and adipogenesis in bone-marrows. Thus, we investigated the effects of specific oxysterols on adipogenesis and expression of adipogenic transcripts in C3H10T1/2 cells. Treatment of cells with DMITro significantly induced mRNA expression of Pparγ. This induction was significantly inhibited by 25-HC. The expression of C/cepα, Fabp4 and Lpl was also inhibited by 25-HC. To determine the mechanism by which 25-HC inhibits adipogenesis, the effects of the hedgehog signalling pathway inhibitor, cyclopamine and CUR61414, were evaluated. Treatment of C3H10T1/2 cells with DMITro + cyclopamine or DMITro + CUR61414 for 96h did not modulate adipocyte differentiation; cyclopamine and CUR61414 did not reverse the inhibitory effects of 25-HC, suggesting that the canonical hedgehog signalling may not play a role in the anti-adipogenic effects of 25-HC in C3H10T1/2 cells. In addition, LXR agonist did not inhibit adipogenesis, but 25-HC strongly inhibits adipogenesis of C3H10T1/2 cells. Our observations showed that 25-HC was the most potent oxysterol in inhibiting adipogenesis and the expression of key adipogenic transcripts in C3H10T1/2 cells among the tested oxysterols, suggesting its potential application in providing an intervention in osteoporosis and obesity. We also report that the inhibitory effects of 25-HC on adipogenic differentiation in C3H10T1/2 cells are not mediated by hedgehog signaling and LXR.
Pilot Study on the Effect of Biophysical Therapy on Salivary Alpha-Amylase as a Surrogate Measure of Anxiety/Stress: In Search of a Novel Noninvasive Molecular Approach for the Management of Stress
Authors: Ferrara Ida , Egan Colin Gerard , Foletti Alberto
Published: January 2020

Anxiety and depression impact dramatically on public health, underlying the importance of alternative cost-effective treatments. Previous studies have shown that biophysical treatment can significantly reduce anxiety symptoms and recently, salivary alpha-amylase (SAA) has been identified as an objective correlate of the sympathetic-parasympathetic imbalance related to increased stress burden, defined as allostatic load. The aim of this study was to evaluate the effect of biophysical therapy on SAA levels, in addition to the Depression Anxiety Stress Scale (DASS)-21 questionnaire. Twenty-four workers (sales representatives) presenting with mild anxiety/stress symptoms (Generalized Anxiety Disorder 7-item scale of > 5) were randomized to biophysical treatment (N = 12) or placebo control (N = 12). The biophysical group underwent electromagnetic information transfer through an aqueous system procedure, with daily self-administration for one month. SAA collection and the DASS-21 questionnaire were undertaken at baseline and after one month in all patients. Clinical characteristics and baseline DASS-21 subscale scores were similar between placebo and biophysical group at baseline. After one month, patients receiving biophysical therapy had significantly reduced SAA levels compared to the placebo group (27.8 ± 39.4 vs. 116.8 ± 114.9 U/mL, p = 0.019). All three DASS-21 subscales, depression (9.3 ± 5.1 vs. 5.7 ± 5.5, p = 0.1), anxiety (6.7 ± 25 vs. 3.7 ± 2.2, p = 0.0049) and stress (10.8 ± 4.2 vs. 7.3 ± 3.7, p = 0.041) were also decreased after biophysical treatment compared to placebo after one month. Our findings suggest that biophysical therapy can benefit workers with mild (subclinical) anxiety/stress. These results were also validated by the concomitant reduction of SAA levels and an improvement in DASS-21 subscales. The underlying molecular mechanisms of this therapy remain to be characterized.
Dynamic Transcriptome Analysis Reveals Uncharacterized Complex Regulatory Pathway Underlying Dose IBA-Induced Embryogenic Redifferentiation in Cotton
Authors: Fan Yupeng , Yu Xiaoman , Guo Huihui , Wei Junmei , Guo Haixia , Zhang Li , Zeng Fanchang
Published: January 2020

The somatic embryogenesis (SE) process of plants is regulated by exogenous hormones. During the SE, different genes sensitively respond to hormone signals through complex regulatory networks to exhibit plant totipotency. When cultured in indole-3-butyric acid (IBA) concentration gradient medium supplemented with 0 mg dm−3, 0.025 mg dm−3, and 0.05 mg dm−3 IBA, the callus differentiation rate first increased then decreased in cotton. To characterize the molecular basis of IBA-induced regulating SE, transcriptome analysis was conducted on embryogenic redifferentiation. Upon the examination of the IBA’s embryogenic inductive effect, it was revealed that pathways related to plant hormone signal transduction and alcohol degradation were significantly enriched in the embryogenic responsive stage (5 days). The photosynthesis, alcohol metabolism and cell cycle pathways were specifically regulated in the pre-embryonic initial period (20 days). Upon the effect of the IBA dose, in the embryogenic responsive stage (5 days), the metabolism of xenobiotics by the cytochrome P450 pathway and secondary metabolism pathways of steroid, flavonoid, and anthocyanin biosynthesis were significantly enriched. The phenylpropanoid, brassinosteroid, and anthocyanin biosynthesis pathways were specifically associated in the pre-embryonic initial period (20 days). At different developmental stages of embryogenic induction, photosynthesis, flavonoid biosynthesis, phenylpropanoid biosynthesis, mitogen-activated protein kinase (MAPK) signaling, xenobiotics metabolism by cytochrome P450, and brassinosteroid biosynthesis pathways were enriched at low a IBA concentration. Meanwhile, at high IBA concentration, the carbon metabolism, alcohol degradation, circadian rhythm and biosynthesis of amino acids pathways were significantly enriched. The results reveal that complex regulating pathways participate in the process of IBA-induced redifferentiation in cotton somatic embryogenesis. In addition, collections of potential essential signaling and regulatory genes responsible for dose IBA-induced efficient embryogenic redifferentiation were identified. Quantitative real-time PCR (qRT-PCR) was performed on the candidate genes with different expression patterns, and the results are basically consistent with the RNA-seq data. The results suggest that the complicated and concerted IBA-induced mechanisms involving multiple cellular pathways are responsible for dose-dependent plant growth regulator-induced SE. This report represents a systematic study and provides new insight into molecular signaling and regulatory basis underlying the process of dose IBA-induced embryogenic redifferentiation during SE.
Significance of 5-S-Cysteinyldopa as a Marker for Melanoma
Authors: Wakamatsu Kazumasa , Fukushima Satoshi , Minagawa Akane , Omodaka Toshikazu , Hida Tokimasa , Hatta Naohito , Takata Minoru , Uhara Hisashi , Okuyama Ryuhei , Ihn Hironobu
Published: January 2020

Melanoma is one of the most lethal and malignant cancers and its incidence is increasing worldwide, and Japan is not an exception. Although there are numerous therapeutic options for melanoma, the prognosis is still poor once it has metastasized. The main concern after removal of a primary melanoma is whether it has metastasized, and early detection of metastatic melanoma would be effective in improving the prognosis of patients. Thus, it is very important to identify reliable methods to detect metastases as early as possible. Although many prognostic biomarkers (mainly for metastases) of melanoma have been reported, there are very few effective for an early diagnosis. Serum and urinary biomarkers for melanoma diagnosis have especially received great interest because of the relative ease of sample collection and handling. Several serum and urinary biomarkers appear to have significant potential both as prognostic indicators and as targets for future therapeutic methods, but still there are no efficient serum and urinary biomarkers for early detection, accurate diagnosis and prognosis, efficient monitoring of the disease and reliable prediction of survival and recurrence. Levels of 5-S-cysteinyldopa (5SCD) in the serum or urine as biomarkers of melanoma have been found to be significantly elevated earlier and to reflect melanoma progression better than physical examinations, laboratory tests and imaging techniques, such as scintigraphy and echography. With recent developments in the treatment of melanoma, studies reporting combinations of 5SCD levels and new applications for the treatment of melanoma are gradually increasing. This review summarizes the usefulness of 5SCD, the most widely used and well-known melanoma marker in the serum and urine, compares 5SCD and other useful markers, and finally its application to other fields.
Production, Structural Characterization, and In Vitro Assessment of the Prebiotic Potential of Butyl-Fructooligosaccharides
Authors: Kang Sini , You Hyun Ju , Lee Yeong-Geun , Jeong Yunju , Johnston Tony V. , Baek Nam-In , Ku Seockmo , Ji Geun Eog
Published: January 2020

Short-chain fatty acids (SCFAs), especially butyrate, produced in mammalian intestinal tracts via fermentation of dietary fiber, are known biofunctional compounds in humans. However, the variability of fermentable fiber consumed on a daily basis and the diversity of gut microbiota within individuals often limits the production of short-chain fatty acids in the human gut. In this study, we attempted to enhance the butyrate levels in human fecal samples by utilizing butyl-fructooligosaccharides (B-FOS) as a novel prebiotic substance. Two major types of B-FOS (GF3-1B and GF3-2B), composed of short-chain fructooligosaccharides (FOS) bound to one or two butyric groups by ester bonds, were synthesized. Qualitative analysis of these B-FOS using Fourier transform infrared (FT-IR) spectroscopy, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), nuclear magnetic resonance (NMR) and low-resolution fast-atom bombardment mass spectra (LR-FAB-MS), showed that the chemical structure of GF3-1B and GF3-2B were [O-(1-buty-β-D-fru-(2→1)-O-β-D-fru-(2→1)-O-β-D-fru-O-α-D-glu] and [O-(1-buty)-β-D-fru-(2→1)-O-β-D-fru-(2→1)-O-(4-buty)-β-D-fru-O-α-D-glu], respectively. The ratio of these two compounds was approximately 5:3. To verify their biofunctionality as prebiotic oligosaccharides, proliferation and survival patterns of human fecal microbiota were examined in vitro via 16S rRNA metagenomics analysis compared to a positive FOS control and a negative control without a carbon source. B-FOS treatment showed different enrichment patterns on the fecal microbiota community during fermentation, and especially stimulated the growth of major butyrate producing bacterial consortia and modulated specific butyrate producing pathways with significantly enhanced butyrate levels. Furthermore, the relative abundance of Fusobacterium and ammonia production with related metabolic genes were greatly reduced with B-FOS and FOS treatment compared to the control group. These findings indicate that B-FOS differentially promotes butyrate production through the enhancement of butyrate-producing bacteria and their metabolic genes, and can be applied as a novel prebiotic compound in vivo.
Vehicle Routing Optimization of Instant Distribution Routing Based on Customer Satisfaction
Authors: Zhang Yan , Yuan Chunhui , Wu Jiang
Published: January 2020

Since the actual factors in the instant distribution service scenario are not considered enough in the existing distribution route optimization, a route optimization model of the instant distribution system based on customer time satisfaction is proposed. The actual factors in instant distribution, such as the soft time window, the pay-to-order mechanism, the time for the merchant to prepare goods before delivery, and the deliveryman’s order combining, were incorporated in the model. A multi-objective optimization framework based on the total cost function and time satisfaction of the customer was established. Dual-layer chromosome coding based on the deliveryman-to-node mapping and the access order was conducted, and the nondominated sorting genetic algorithm version II (NSGA-II) was used to solve the problem. According to the numerical results, when time satisfaction of the customer was considered in the instant distribution routing problem, the customer satisfaction increased effectively and the balance between customer satisfaction and delivery cost in the means of Pareto optimization were obtained, with a minor increase in the delivery cost, while the number of deliverymen slightly increased to meet the on-time delivery needs of customers.
Opportunistic Multi-Technology Cooperative Scheme and UAV Relaying for Network Disaster Recovery
Authors: Mezghani Farouk , Mitton Nathalie
Published: January 2020

Disaster scenarios are particularly catastrophic in urban environments, which are very densely populated in many cases. Disasters not only endanger the life of people, but also affect the existing communication infrastructures. In fact, such an infrastructure could be completely destroyed or damaged; even when it continues working, it suffers from high access demand to its limited resources within a short period of time. This work evaluates the performances of smartphones and leverages the ubiquitous presence of mobile devices in urban scenarios to assist search and rescue activities following a disaster. Specifically, it proposes a collaborative protocol that opportunistically organizes mobile devices in multiple tiers by targeting a fair energy consumption in the whole network. Moreover, it introduces a data collection scheme that employs drones to scan the disaster area and to visit mobile devices and collect their data in a short time. Simulation results in realistic settings show that the proposed solution balances the energy consumption in the network by means of efficient drone routes and smart self-organization, thereby effectively assisting search and rescue operations.
Predictors for Target Vessel Failure after Recanalization of Chronic Total Occlusions in Patients Undergoing Surveillance Coronary Angiography
Authors: Geyer Martin , Wild Johannes , Hirschmann Marc , Dimitriadis Zisis , Münzel Thomas , Gori Tommaso , Wenzel Philip
Published: January 2020

(1) Background: Knowledge about predictors for the long-time patency of recanalized chronic total coronary occlusions (CTOs) is limited. Evidence from invasive follow-up in the absence of acute coronary syndrome (routine surveillance coronary angiography) is scarce. (2) Methods: In a monocentric-retrospective analysis, we obtained baseline as well as periprocedural data of patients undergoing routine invasive follow-up. We defined target vessel failure (TVF) as a combined primary endpoint, consisting of re-occlusion, restenosis, and target vessel revascularization (TVR). (3) Results: We included 93 consecutive patients (15.1% female) from October 2013 to May 2018. After a follow-up period of 206 ± 129 days (median 185 (IQR 127–237)), re-occlusion had occurred in 7.5%, restenosis in 11.8%, and TVR in 5.4%; the cumulative incidence of TVF was 15.1%. Reduced TIMI-flow immediately after recanalization (OR for TVR: 11.0 (95% CI: 2.7–45.5), p = 0.001) as well as female gender (OR for TVR: 11.0 (95% CI: 2.1–58.5), p = 0.005) were found to be predictive for pathological angiographic findings at follow-up. Furthermore, higher blood values of high-sensitive troponin after successful revascularization were associated with all endpoints. Interestingly, neither the J-CTO score nor the presence of symptoms at the follow-up visit could be correlated to adverse angiographic results. (4) Conclusions: In this medium-sized cohort of patients with surveillance coronary angiography, we were able to identify reduced TIMI flow and female gender as the strongest predictors for future TVF.
Chimeric Antigen Receptor T-Cell Therapy for Colorectal Cancer
Authors: Sur Daniel , Havasi Andrei , Cainap Calin , Samasca Gabriel , Burz Claudia , Balacescu Ovidiu , Lupan Iulia , Deleanu Diana , Irimie Alexandru
Published: January 2020

Chimeric antigen receptor (CAR) T-cell therapy represents a new genetically engineered method of immunotherapy for cancer. The patient’s T-cells are modified to express a specific receptor that sticks to the tumor antigen. This modified cell is then reintroduced into the patient’s body to fight the resilient cancer cells. After exhibiting positive results in hematological malignancies, this therapy is being proposed for solid tumors like colorectal cancer. The clinical data of CAR T-cell therapy in colorectal cancer is rather scarce. In this review, we summarize the current state of knowledge, challenges, and future perspectives of CAR T-cell therapy in colorectal cancer. A total of 22 articles were included in this review. Eligible studies were selected and reviewed by two researchers from 49 articles found on Pubmed, Web of Science, and clinicaltrials.gov. This therapy, at the moment, provides modest benefits in solid tumors. Not taking into consideration the high manufacturing and retail prices, there are still limitations like increased toxicities, relapses, and unfavorable tumor microenvironment for CAR T-cell therapy in colorectal cancer.
Diagnostic Accuracy of Stool Tests for Colorectal Cancer Surveillance in Hodgkin Lymphoma Survivors
Authors: Ykema Berbel , Rigter Lisanne , Spaander Manon , Moons Leon , Bisseling Tanya , Aleman Berthe , de Boer Jan Paul , Lugtenburg Pieternella , Janus Cecile , Petersen Eefke , Roesink Judith , Raemaekers John , van der Maazen Richard , Lansdorp-Vogelaar Iris , Gini Andrea , Verbeek Wieke , Lemmens Margriet , Meijer Gerrit , van Leeuwen Flora , Snaebjornsson Petur , Carvalho Beatriz , van Leerdam Monique
Published: January 2020

Background: Hodgkin lymphoma (HL) survivors have an increased colorectal cancer (CRC) risk. Diagnostic accuracy of quantitative fecal immunochemical testing (FIT, OC Sensor) and/or a multi-target stool DNA test (mt-sDNA, Cologuard®) for advanced neoplasia (AN) was evaluated. Methods: 101 HL survivors underwent a surveillance colonoscopy and were asked to perform two stool tests (FIT and mt-sDNA). Advanced adenoma (AA), advanced serrated lesion (ASL), and AN (AA, ASL, CRC) were evaluated. Sensitivity, specificity, and area under the curve (AUC) for AN were calculated for different FIT cut-offs and mt-sDNA with colonoscopy as reference. Results: FIT and mt-sDNA were analyzed in 73 (72%) and 82 (81%) participants, respectively. AN was detected in 19 (26%) and 22 (27%), respectively. AN sensitivities for FIT cut-off of 10 ug Hb/g feces (FIT10) and mt-sDNA were 37% (95% confidence interval (CI): 16–62) and 68% (95% CI: 45–86), with corresponding specificities of 91% (95% CI: 80–97) and 70% (95% CI: 57–86), respectively. AUC for FIT was 0.68 (95% CI: 0.54–0.82) and for mt-sDNA 0.76 (95% CI: 0.63–0.89). Conclusions: In HL survivors, mt-sDNA showed highest sensitivity but with relatively low specificity for AN. Cost-effectiveness analyses is necessary to determine the optimal surveillance strategy.
Persistent Inflammation, Immunosuppression and Catabolism Syndrome (PICS) after Polytrauma: A Rare Syndrome with Major Consequences
Authors: Hesselink Lillian , Hoepelman Ruben J. , Spijkerman Roy , de Groot Mark C. H. , van Wessem Karlijn J. P. , Koenderman Leo , Leenen Luke P. H. , Hietbrink Falco
Published: January 2020

Nowadays, more trauma patients develop chronic critical illness (CCI), a state characterized by prolonged intensive care. Some of these CCI patients have disproportional difficulties to recover and suffer from recurrent infections, a syndrome described as the persistent inflammation, immunosuppression and catabolism syndrome (PICS). A total of 78 trauma patients with an ICU stay of ≥14 days (CCI patients) between 2007 and 2017 were retrospectively included. Within this group, PICS patients were identified through two ways: (1) their clinical course (≥3 infectious complications) and (2) by laboratory markers suggested in the literature (C-reactive protein (CRP) and lymphocytes), both in combination with evidence of increased catabolism. The incidence of PICS was 4.7 per 1000 multitrauma patients. The sensitivity and specificity of the laboratory markers was 44% and 73%, respectively. PICS patients had a longer hospital stay (median 83 vs. 40, p < 0.001) and required significantly more surgical interventions (median 13 vs. 3, p = 0.003) than other CCI patients. Thirteen PICS patients developed sepsis (72%) and 12 (67%) were readmitted at least once due to an infection. In conclusion, patients who develop PICS experience recurrent infectious complications that lead to prolonged hospitalization, many surgical procedures and frequent readmissions. Therefore, PICS forms a substantial burden on the patient and the hospital, despite its low incidence.
Time Domain Modeling of Propeller Forces due to Ventilation in Static and Dynamic Conditions
Authors: Kozlowska Anna Maria , Dalheim Øyvind Øksnes , Savio Luca , Steen Sverre
Published: January 2020

This paper presents experimental and theoretical studies on the dynamic effect on the propeller loading due to ventilation by using a simulation model that generates a time domain solution for propeller forces in varying operational conditions. For ventilation modeling, the simulation model applies a formula based on the idea that the change in lift coefficient due to ventilation computes the change in the thrust coefficient. It is discussed how dynamic effects, like hysteresis effects and blade frequency dynamics, can be included in the simulation model. Simulation model validation was completed by comparison with CFD (computational fluid dynamics) calculations and model experiments. Experiments were performed for static and dynamic (heave motion) conditions in the large towing tank at the SINTEF Ocean in Trondheim and in the Marine Cybernetics Laboratories at NTNU (Norwegian University of Science and Technology). The main focus of this paper is to explain and validate the prediction model for thrust loss due to ventilation and out of water effects in static and dynamic heave conditions.