bluuebunny/crossref_metadata_2025 huggingface dataset:
Cost-Effectiveness Analysis of Abemaciclib Plus Fulvestrant in the Second-Line Treatment of Women With HR+/HER2– Advanced or Metastatic Breast Cancer: A US Payer Perspective
Authors: Wang Yingcheng , Rui Mingjun , Guan Xin , Cao Yingdan , Chen Pingyu
Published: June 2021

Introduction:This study evaluated the cost-effectiveness of abemaciclib plus fulvestrant (ABE + FUL) vs. palbociclib plus fulvestrant (PAL + FUL), ribociclib plus fulvestrant (RIB + FUL) and fulvestrant monotherapy (FUL) as second-line treatment for hormone receptor-positive and human epidermal growth factor receptor 2- negative advanced or metastatic breast cancer in the US.

Methods:The 3 health states partitioned survival (PS) model was used over the lifetime. Effectiveness and safety data were derived from the MONARCH 2 trial, MONALEESA-3 trial, and PALOMA-3 trial. Parametric survival models were used for four treatments to explore the long-term effect. Costs were derived from the pricing files of Medicare and Medicaid Services, and utility values were derived from published studies. Sensitivity analyses including one-way sensitivity analysis, probabilistic sensitivity analysis and scenario analysis were performed to observe model stability.

Results:In the PS model, compared with PAL + FUL, ABE + FUL yielded 0.44 additional QALYs at an additional cost of $100,696 for an incremental cost-utility ratio (ICUR) of $229,039/QALY. Compared with RIB + FUL, ABE + FUL yielded 0.03 additional QALYs at an additional cost of $518 for an ICUR of $19,314/QALY. Compared with FUL, ABE + FUL yielded 0.68 additional QALYs at an additional cost of $260,584 for ICUR of $381,450/QALY. From the PS model, the ICUR was $270,576 /QALY (ABE + FUL vs. PAL + FUL), dominated (ABE + FUL vs. RIB + FUL) and $404,493/QALY (ABE + FUL vs. FUL) in scenario analysis. In the probabilistic sensitivity analysis, the probabilities that ABE + FUL was cost-effective vs. PAL + FUL, RIB + FUL and FUL at thresholds of $50,000, $100,000, and $200,000 per QALY gained were 0% and the probabilities that ABE + FUL was cost-effective vs. PAL + FUL and RIB + FUL at thresholds of $50,000, $100,000, and $200,000 per QALY gained were 0.2, 0.6, and 7.3%.

Conclusions:The findings from the present analysis suggest that ABE + FUL might be cost-effective compared with RIB + FUL and not cost-effective compared with PAL + FUL and FUL for second-line treatment of patients with HR+/HER2– advanced or metastatic breast cancer in the US.
Quinoline Antimalarials Increase the Antibacterial Activity of Ampicillin
Authors: Olateju Olajumoke A. , Babalola Chinedum P. , Olubiyi Olujide O. , Kotila Olayinka A. , Kwasi David A. , Oaikhena Anderson O. , Okeke Iruka N.
Published: June 2021

Bacterial and malaria co-infections are common in malaria endemic countries and thus necessitate co-administration of antibiotics and antimalarials. There have long been anecdotal clinical reports of interactions between penicillins and antimalarial agents, but the nature and mechanisms of these interactions remain to be investigated. In this study, we employed antimicrobial interaction testing methods to study the effect of two antimalarials on the antibacterial activity of ampicillin in vitro. Paper strip diffusion, a modified disc diffusion and checkerboard methods were used to determine the nature of interactions between ampicillin and quinoline antimalarials, chloroquine and quinine, against Gram-positive and Gram-negative bacteria. The impact of antimalarials and ampicillin-antimalarial drug combinations on cell integrity of test bacteria were determined by measuring potassium release. The tested antimalarials did not show substantial antibacterial activity but quinine was bactericidal at high concentrations. Chloroquine and quinine increased ampicillin activity, with increasing concentrations extending the antibacterial’s inhibition zones by 2.7-4.4 mm and from 1.1 to over 60 mm, respectively. Observed interactions were largely additive with Fractional Inhibitory Concentration Indices of >0.5-1 for all ampicillin-antimalarial combinations. Quinine and, to a lesser extent, chloroquine increase the activity of ampicillin and potentially other β-lactams, which has implications for combined clinical use.
Genome and Evolutionary Analysis of Nosema ceranae: A Microsporidian Parasite of Honey Bees
Authors: Huang Qiang , Wu Zhi Hao , Li Wen Feng , Guo Rui , Xu Jin Shan , Dang Xiao Qun , Ma Zheng Gang , Chen Yan Ping , Evans Jay D.
Published: June 2021

Microsporidia comprise a phylum of single cell, intracellular parasites and represent the earliest diverging branch in the fungal kingdom. The microsporidian parasite Nosema ceranae primarily infects honey bee gut epithelial cells, leading to impaired memory, suppressed host immune responses and colony collapse under certain circumstances. As the genome of N. ceranae is challenging to assembly due to very high genetic diversity and repetitive region, the genome was re-sequenced using long reads. We present a robust 8.8 Mbp genome assembly of 2,280 protein coding genes, including a high number of genes involved in transporting nutrients and energy, as well as drug resistance when compared with sister species Nosema apis. We also describe the loss of the critical protein Dicer in approximately half of the microsporidian species, giving new insights into the availability of RNA interference pathway in this group. Our results provided new insights into the pathogenesis of N. ceranae and a blueprint for treatment strategies that target this parasite without harming honey bees. The unique infectious apparatus polar filament and transportation pathway members can help to identify treatments to control this parasite.
The Use of Ribosomal RNA as a Microbial Source Tracking Target Highlights the Assay Host-Specificity Requirement in Water Quality Assessments
Authors: Rytkönen Annastiina , Tiwari Ananda , Hokajärvi Anna-Maria , Uusheimo Sari , Vepsäläinen Asko , Tulonen Tiina , Pitkänen Tarja
Published: June 2021

For microbial source tracking (MST), the 16S ribosomal RNA genes (rDNA) of host-specific bacteria and mitochondrial DNA (mtDNA) of animal species, known to cause fecal contamination of water, have been commonly used as molecular targets. However, low levels of contamination might remain undetected by using these DNA-based qPCR assays. The high copy numbers of ribosomal RNA (rRNA) could offer a solution for such applications of MST. This study compared the performance of eight MST assays: GenBac3 (general Bacteroidales), HF183 (human), BacCan (dog), Rum-2-Bac (ruminant), Pig-2-Bac (swine), Gull4 (gull), GFD, and Av4143 (birds) between rRNA-based and rDNA-based approaches. Three mtDNA-based approaches were tested: DogND5, SheepCytB, and HorseCytB. A total of 151 animal fecal samples and eight municipal sewage samples from four regions of Finland were collected for the marker evaluation. The usability of these markers was tested by using a total of 95 surface water samples with an unknown pollution load. Overall, the performance (specificity, sensitivity, and accuracy) of mtDNA-based assays was excellent (95–100%), but these markers were very seldom detected from the tested surface water samples. The rRNA template increased the sensitivity of assays in comparison to the rDNA template. All rRNA-based assays (except Av4143) had more than 80% sensitivity. In contrast, only half (HF183, Rum-2-Bac, Pig-2-Bac, and Gull4) of rDNA-based assays reached this value. For markers targeted to bird feces, the use of the rRNA-based assay increased or at least did not change the performance. Regarding specificity, all the assays had >95% specificity with a DNA template, except the BacCan assay (71%). While using the RNA template for the assays, HF183 and BacCan exhibited only a low level of specificity (54 and 55%, respectively). Further, the HF183 assay amplified from multiple non-targeted animal fecal samples with the RNA template and the marker showed cross-amplification with the DNA template as well. This study recommends using the rRNA-based approach for MST assays targeting bird fecal contamination. In the case of mammal-specific MST assays, the use of the rRNA template increases the sensitivity but may reduce the specificity and accuracy of the assay. The finding of increased sensitivity calls for a further need to develop better rRNA-based approaches to reach the required assay performance.
Effectiveness of the Auditory Temporal Ordering and Resolution Tests to Detect Central Auditory Processing Disorder in Adults With Evidence of Brain Pathology: A Systematic Review and Meta-Analysis
Authors: Chowsilpa Sanathorn , Bamiou Doris-Eva , Koohi Nehzat
Published: June 2021

Background: Auditory temporal processing tests are key clinical measures in order to diagnose central auditory processing disorder (CAPD). Although these tests have been used for decades, there is no up-to-date evidence to determine the effectiveness of detecting the abnormalities in central auditory processing in adults while the available national CAPD guidelines predominantly address CAPD in the pediatric population.

Purpose: To determine the efficacy of the auditory temporal ordering tests [duration pattern test (DPT) and frequency pattern test (FPT)], and a temporal resolution test [gaps-in-noise (GIN) test] for detecting the central auditory processing abnormalities in adults with documented brain pathology.

Research Design: Systematic reviews and meta-analyses.

Study samples: Four databases, including PubMed, Web of Science, Embase, and Scopus, were systematically searched. The publications in the English language that recruited adults (above 16 years old) with pathologic brain conditions and described the diagnostic tests for auditory temporal processing were selected for review.

Data Collections and Analysis: All data were systematically evaluated, extracted, categorized, and summarized in tables. The meta-analysis was done in order to determine the effectiveness of the DPT, FPT, and GIN tests.

Results: The results showed significantly poorer performance of DPT and FPT, compared between participants with confirmed brain disease and normal controls, at the mean differences of percent correct −21.93 (95% CI, −26.58 to −17.29) and −31.37 (95% CI, −40.55 to −22.19), respectively. Subjects with brain pathology also performed poorer in GIN test at the mean difference of 3.19 milliseconds (95% CI, 2.51 to 3.87).

Conclusion: The results from the meta-analysis provide evidence that DPT, FPT, and GIN clinical measures are effective in the diagnosis of CAPD in adults with neurological disorders. Poor performance on these tests is significantly related to the confirmed brain pathology. However, different units in results presentation and variety of testing strategies are limitations for this meta-analysis. The standard pattern of result reporting and international protocols test strategies should be developed in order to conduct better meta-analyses with a larger collection of suitable studies and less heterogeneity.
Effects of Bilateral Transcranial Direct Current Stimulation on Simultaneous Bimanual Handgrip Strength
Authors: Hikosaka Mikito , Aramaki Yu
Published: June 2021

Although the effects of transcranial direct current stimulation (tDCS) on contralateral unimanual movement have been well reported, its effects on coordinated multi-limb movements remain unclear. Because multi-limb coordination is often performed in daily activities and sports, clarifying the effects of tDCS on multi-limb coordination may have valuable implications. However, considering the neural crosstalk involved in bimanual movements, including the transcallosal pathway and ipsilateral motor pathway, the extent of tDCS-induced improvement may differ between unimanual and bimanual movement. We examined how tDCS affects simultaneous bimanual maximal voluntary contraction (MVC) by testing the effects of tDCS of the bilateral primary motor cortex (M1) on unimanual and bimanual handgrip strength. Twenty-one right-handed healthy adults underwent three bilateral tDCS protocols (“RaLc,” with an anode on right M1 and a cathode on left M1, “RcLa,” with an anode on left M1 and a cathode on right M1, and “Sham”) in a randomized order. A 1.5 mA current was applied for 15 min during tDCS. Participants then performed maximal unimanual and bimanual handgrip tests. Bimanual handgrip force was higher in both hands in the RcLa condition than in the Sham condition. Similarly, unimanual handgrip force was higher in the RcLa condition than in the Sham condition. Stimulus responses were asymmetrical and were not observed in the RaLc condition. Our findings demonstrate that RcLa tDCS leads to neuromodulation that can produce greater unimanual and bimanual handgrip strength. This result provides basic evidence that tDCS may be useful in sports, particularly those involving bilateral coordination of upper limb movement.
Low-Cutoff Frequency Reduction in Neural Amplifiers: Analysis and Implementation in CMOS 65 nm
Authors: Hashemi Noshahr Fereidoon , Nabavi Morteza , Gosselin Benoit , Sawan Mohamad
Published: June 2021

Scaling down technology demotes the parameters of AC-coupled neural amplifiers, such as increasing the low-cutoff frequency due to the short-channel effects. To improve the low-cutoff frequency, one solution is to increase the feedback capacitors' value. This solution is not desirable, as the input capacitors have to be increased to maintain the same gain, which increases the area and decreases the input impedance of the neural amplifier. We analytically analyze the small-signal behavior of the neural amplifier and prove that the main reason for the increase of the low-cutoff frequency in advanced CMOS technologies is the reduction of the input resistance of the operational transconductance amplifier (OTA). We also show that the reduction of the input resistance of the OTA is due to the increase in the gate oxide leakage in the input transistors. In this paper, we explore this fact and propose two solutions to reduce the low-cutoff frequency without increasing the value of the feedback capacitor. The first solution is performed by only simulation and is called cross-coupled positive feedback that uses pseudoresistors to provide a negative resistance to increase the input resistance of the OTA. As an advantage, only standard CMOS transistors are used in this method. Simulation results show that a low-cutoff frequency of 1.5 Hz is achieved while the midband gain is 30.4 dB at 1 V. In addition, the power consumption is 0.6 μW. In the second method, we utilize thick-oxide MOS transistors in the input differential pair of the OTA. We designed and fabricated the second method in the 65 nm TSMC CMOS process. Measured results are obtained by in vitro recordings on slices of mouse brainstem. The measurement results show that the bandwidth is between 2 Hz and 5.6 kHz. The neural amplifier has 34.3 dB voltage gain in midband and consumes 3.63 μW at 1 V power supply. The measurement results show an input-referred noise of 6.1 μVrms and occupy 0.04 mm2 silicon area.
A Nomogram for Predicting Survival in Patients With Breast Cancer Liver Metastasis: A Population-Based Study
Authors: Xiong Yu , Shi Xia , Hu Qi , Wu Xingwei , Long Enwu , Bian Yuan
Published: June 2021

Objective

The prognosis of patients with breast cancer liver metastasis (BCLM) was poor. We aimed at constructing a nomogram to predict overall survival (OS) for BCLM patients using the SEER (Surveillance Epidemiology and End Results) database, thus choosing an optimized therapeutic regimen to treat.

Methods

We identified 1173 patients with BCLM from the SEER database and randomly divided them into training (n=824) and testing (n=349) cohorts. The Cox proportional hazards model was applied to identify independent prognostic factors for BCLM, based on which a nomogram was constructed to predict 1-, 2-, and 3-year OS. Its discrimination and calibration were evaluated by the Concordance index (C-index) and calibration plots, while the accuracy and benefits were assessed by comparing it to AJCC-TNM staging system using the decision curve analysis (DCA). Kaplan-Meier survival analyses were applied to test the clinical utility of the risk stratification system.

Results

Grade, marital status, surgery, radiation therapy, chemotherapy, CS tumor size, tumor subtypes, bone metastatic, brain metastatic, and lung metastatic were identified to be independent prognostic factors of OS. In comparison with the AJCC-TNM staging system, an improved C-index was obtained (training group: 0.701 vs. 0.557, validation group: 0.634 vs. 0.557). The calibration curves were consistent between nomogram-predicted survival probability and actual survival probability. Additionally, the DCA curves yielded larger net benefits than the AJCC-TNM staging system. Finally, the risk stratification system can significantly distinguish the ones with different survival risk based on the different molecular subtypes.

Conclusion

We have successfully built an effective nomogram and risk stratification system to predict OS in BCLM patients, which can assist clinicians in choosing the appropriate treatment strategies for individual BCLM patients.
Lactate Modulates Cellular Metabolism Through Histone Lactylation-Mediated Gene Expression in Non-Small Cell Lung Cancer
Authors: Jiang Jun , Huang DengLiang , Jiang Yuan , Hou Jing , Tian MeiYuan , Li JianHua , Sun Li , Zhang YaoGang , Zhang Tao , Li ZhiQin , Li ZhongCheng , Tong SiXian , Ma YanYan
Published: June 2021

Lactate has been observed to fuel TCA cycle and is associated with cancer progression in human lung cancer, the leading cause of cancer deaths worldwide, but the effect of lactate on lung cancer metabolism is rarely reported. In this study, disordered metabolism in non-small cell lung cancer was demonstrated by increased G6PD and SDHA protein levels via immunofluorescence, and up-regulated lactate dehydrogenase was found to be associated with poor prognosis. Then flow cytometry and Seahorse XFe analyzer were utilized to detect the effect of lactate on glycolysis and mitochondrial function in non-small cell lung cancer cells. The results show that in non-small cell lung cancer cells lactate attenuates glucose uptake and glycolysis while maintaining mitochondrial homeostasis as indicated by improved mitochondrial membrane potential. Further exploration found that mRNA levels of glycolytic enzymes (HK-1, PKM) and TCA cycle enzymes (SDHA, IDH3G) are respectively down-regulated and up-regulated by lactate, and increased histone lactylation was observed in promoters of HK-1 and IDH3G via chromatin immunoprecipitation assay. Taken together, the above results indicate that lactate modulates cellular metabolism at least in part through histone lactylation-mediated gene expression in non-small cell lung cancer.
Continuous Complete Remission in Two Patients with Acute Lymphoblastic Leukemia and Severe Fungal Infection Following Short-Term, Dose-Reduced Chemotherapy
Authors: Lüke Florian , Harrer Dennis C. , Hahn Joachim , Grube Matthias , Pukrop Tobias , Herr Wolfgang , Reichle Albrecht , Heudobler Daniel
Published: June 2021

Spontaneous remission in acute lymphoblastic leukemia (ALL) is a rare phenomenon, which typically involves a pattern of feverish or septic disease followed by quick but mostly transient remission. We report on two male patients (46-year-old (pt. 1) and 19-year-old (pt. 2)) with CD20 positive, BCR-ABL negative common B-ALL. Patient 1 had received dexamethasone and cyclophosphamide (1.2 g) as a prephase therapy, followed by rituximab and a cumulative dose of 200 mg daunorubicin combined with 2 mg vincristine as an induction therapy. Patient 2 was treated with a reduced therapy regimen (Vincristine 1 mg, dexamethasone and 80 mg daunorubicin, 12-month mercaptopurine maintenance) due to (alcohol-related) toxic liver failure and pontine myelinolysis. Both patients developed severe septic disease just few days into induction treatment. Patient 1 suffered from pulmonary mycosis, which had to be resected eventually. Histological work-up revealed invasive mucor mycosis. Patient 2 presented with elevated serum aspergillus antigen and radiographic pulmonary lesions, indicative of pulmonary mycosis. In both patients, chemotherapy had to be interrupted and could not be resumed. Both patients recovered under broad antimicrobial, antifungal and prophylactic antiviral therapy and achieved molecular complete remission. At data cut-off remissions had been on-going for 34 months (pt. 1) and 8 years (pt. 2). Short-term, reduced intensity induction chemotherapy accompanied by severe fungal infections was followed by long-lasting continuous complete remissions in ALL. Thus, we hypothesize that infection-associated immunogenic responses may not only prevent early relapse of ALL but could also eradicate minimal residual disease. The effects of combined cytotoxic therapy and severe infection may also be mimicked by biomodulatory treatment strategies aiming at reorganizing pathologically altered cellular signaling networks. This could reduce toxicity and comorbidity in adult patients requiring leukemia treatment. Therefore, these two cases should encourage systematic studies on how leukemia stroma interaction can be harnessed to achieve long lasting control of ALL.
Bio-Guided Fractionation and Molecular Networking Reveal Fatty Acids to Be Principal Anti-Parasitic Compounds in Nordic Seaweeds
Authors: Bonde Charlotte Smith , Bornancin Louis , Lu Yi , Simonsen Henrik Toft , Martínez-Valladares María , Peña-Espinoza Miguel , Mejer Helena , Williams Andrew R. , Thamsborg Stig Milan
Published: June 2021

Widespread use of antimicrobial drugs has led to high levels of drug-resistance in pathogen populations and a need for novel sources of anti-bacterial and anti-parasitic compounds. Macroalgae (seaweed) are potentially a rich source of bioactive compounds, and several species have traditionally been used as vermifuges. Here, we investigated the anti-parasitic properties of four common cold-water Nordic seaweeds; Palmaria palmata (Rhodophyta), Laminaria digitata, Saccharina latissima and Ascophyllum nodosum (Ochrophyta, Phaeophyceae). Screening of organic extracts against helminths of swine (Ascaris suum) and sheep (Teladorsagia circumcincta) revealed that S. latissima and L. digitata had particularly high biological activity. A combination of molecular networking and bio-guided fractionation led to the isolation of six compounds from extracts of these two species identified in both fermented and non-fermented samples. The six isolated compounds were tentatively identified by using MS-FINDER as five fatty acids and one monoglyceride: Stearidonic acid (1), Eicosapentaenoic acid (2), Alpha-Linolenic acid (3), Docosahexaenoic acid (4), Arachidonic acid (5), and Monoacylglycerol (MG 20:5) (6). Individual compounds showed only modest activity against A. suum, but a clear synergistic effect was apparent when selected compounds were tested in combination. Collectively, our data reveal that fatty acids may have a previously unappreciated role as natural anti-parasitic compounds, which suggests that seaweed products may represent a viable option for control of intestinal helminth infections.
Collective Movement and Collective Information Acquisition With Signaling
Authors: Salahshour Mohammad , Rouhani Shahin
Published: June 2021

We consider a population of mobile agents able to make noisy observations of the environment and communicate their observation by production and comprehension of signals. Individuals try to align their movement direction with their neighbors. Besides, they try to collectively find and travel towards an environmental direction. We show that, when the fraction of informed individuals is small, by increasing the noise in communication, similarly to the Vicsek model, the model shows a discontinuous order-disorder transition with strong finite-size effects. In contrast, for a large fraction of informed individuals, it is possible to go from the ordered phase to the disordered phase without passing any phase transition. The ordered phase is composed of two phases separated by a discontinuous transition. Informed collective motion, in which the population collectively infers the correct environmental direction, occurs for a high fraction of informed individuals. When the fraction of informed individuals is low, the misinformed collective motion, where the population fails to find the environmental direction, becomes stable as well. Besides, we show that an amount of noise in the production of signals is more detrimental for the inference capability of the population and increases temporal fluctuations, the density fluctuations, and the probability of group fragmentation, compared to the same amount of noise in the comprehension.
A Robust and Rapid Candidate Gene Mapping Pipeline Based on M2 Populations
Authors: Zhou Huangkai , Tang Kuanqiang , Li Guang , Liu Wenqiang , Yu Hui , Yuan Xiaohui , Yang Suxin , Bhattacharyya Madan K. , Feng Xianzhong
Published: June 2021

The whole-genome sequencing-based bulked segregant analysis (WGS-BSA) has facilitated the mapping candidate causal variations for cloning target plant genes. Here, we report an improved WGS-BSA method termed as M2-seq to expedite the mapping candidate mutant loci by studying just M2 generation. It is an efficient mutant gene mapping tool, rapid, and comparable to the previously reported approaches, such as Mutmap and Mutmap+ that require studying M3 or advanced selfed generations. In M2-seq, background variations among the M2 populations can be removed efficiently without knowledge of the variations of the wild-type progenitor plant. Furthermore, the use of absolute delta single-nucleotide polymorphism (SNP) index values can effectively remove the background variation caused by repulsion phase linkages of adjacent mutant alleles; and thereby facilitating the identification of the causal mutation in target genes. Here, we demonstrated the application of M2-seq in successfully mapping the genomic regions harboring causal mutations for mutant phenotypes among 10 independent M2 populations of soybean. The mapping candidate mutant genes just in M2 generation with the aid of the M2-seq method should be particularly useful in expediting gene cloning especially among the plant species with long generation time.
Bodily Information and Top-Down Affective Priming Jointly Affect the Processing of Fearful Faces
Authors: Yu Alessandra Nicoletta Cruz , Iodice Pierpaolo , Pezzulo Giovanni , Barca Laura
Published: June 2021

According to embodied theories, the processing of emotions such as happiness or fear is grounded in emotion-specific perceptual, bodily, and physiological processes. Under these views, perceiving an emotional stimulus (e.g., a fearful face) re-enacts interoceptive and bodily states congruent with that emotion (e.g., increases heart rate); and in turn, interoceptive and bodily changes (e.g., increases of heart rate) influence the processing of congruent emotional content. A previous study by Pezzulo et al. (2018) provided evidence for this embodied congruence, reporting that experimentally increasing heart rate with physical exercise facilitated the processing of facial expressions congruent with that interoception (fear), but not those conveying incongruent states (disgust or neutrality). Here, we investigated whether the above (bottom-up) interoceptive manipulation and the (top-down) priming of affective content may jointly influence the processing of happy and fearful faces. The fact that happiness and fear are both associated with high heart rate but have different (positive and negative) valence permits testing the hypothesis that their processing might be facilitated by the same interoceptive manipulation (the increase of heart rate) but two opposite (positive and negative) affective primes. To test this hypothesis, we asked participants to perform a gender-categorization task of happy, fearful, and neutral faces, which were preceded by positive, negative, and neutral primes. Participants performed the same task in two sessions (after rest, with normal heart rate, or exercise, with faster heart rate) and we recorded their response times and mouse movements during the choices. We replicated the finding that when participants were in the exercise condition, they processed fearful faces faster than when they were in the rest condition. However, we did not find the same reduction in response time for happy (or neutral) faces. Furthermore, we found that when participants were in the exercise condition, they processed fearful faces faster in the presence of negative compared to positive or neutral primes; but we found no equivalent facilitation of positive (or neutral) primes during the processing of happy (or neutral) faces. While the asymmetries between the processing of fearful and happy faces require further investigation, our findings promisingly indicate that the processing of fearful faces is jointly influenced by both bottom-up interoceptive states and top-down affective primes that are congruent with the emotion.
Undergraduate Students’ Perceived Stress Levels in Summer Term 2020 – A Comparison to Preceding Academic Terms
Authors: Goppert Simone Antje , Pfost Maximilian
Published: June 2021

The COVID-19 pandemic tremendously affected teaching and learning in both schools and higher education settings. In Germany, university students had to shift from in-person group learning in lectures and seminars to new forms of e-learning and distance teaching. Even before COVID-19, stress was a common experience among university students, and these changes have reinforced students’ stress levels. Based on a sample of n = 110 German university students, this study explores whether students’ perceived stress levels in summer term 2020 differed from their perceived stress levels in preceding academic terms. The results show that students experienced lower levels of stress and higher levels of joy in summer term 2020 compared to preceding academic terms. Despite limitations in the interpretation of these findings, possible explanations, such as changes in academic and non-academic workload or decreased demands in university exams, are discussed.
Case Report: Dynamic Interdependencies Between Complementary and Alternative Medicine (CAM) Practice, Urinary Interleukin-6 Levels, and Fatigue in a Breast Cancer Survivor
Authors: Singer Magdalena , Ott Michaela , Bliem Harald R. , Hladschik-Kermer Birgit , Ocaña-Peinado Francisco M. , Chamson Emil , Schubert Christian
Published: June 2021

Background: This study investigated the influence of complementary and alternative medicine (CAM) techniques (i.e., Jin Shin Jyutsu, music, physiotherapy, Tai Chi, and energy healing) on urinary interleukin-6 (IL-6) levels and fatigue in a 49-year-old breast cancer survivor suffering from cancer-related fatigue and depression. Data were sampled under conditions of “life as it is lived.”

Methods: For 28 days, a female breast cancer survivor collected her full urine output in 12-h intervals from about 8 a.m. to 8 p.m. and from about 8 p.m. to 8 a.m. These urine samples were used to determine urinary IL-6 levels through ELISA and creatinine concentrations via HPLC. In 12-h intervals (every morning and evening), the patient completed the DIARI, which included fatigue measurement and notes on incidents and activities such as CAM practice. In addition, the patient was interviewed weekly to identify meaningful everyday incidents. In this context, CAM practice was also discussed. Time series analysis consisted of ARIMA modeling and cross-correlational analyses (p < 0.05).

Results: When each CAM technique was considered separately in time series analysis, CAM was consistently associated with increases in urinary IL-6 release and decreases in fatigue. Furthermore, when all CAM techniques experienced as positive were included in one time series, a biphasic urinary IL-6 response pattern was found in which CAM practice was first preceded by decreases in IL-6 by 12–0 h and then followed by increases in IL-6 after 108–120 h. Finally, cross-correlations between IL-6 and fatigue showed that increases in IL-6 were followed by decreases in fatigue intensity after 48–60 h and, conversely, that decreases in fatigue intensity were followed by decreases in IL-6 after 24–36 h and 48–60 h.

Conclusion: IL-6 increases and fatigue decreases highlight potential health-promoting effects of CAM practice. Moreover, a cyclic IL-6 pattern in response to all CAM activities experienced as positive underscores that CAM was meaningful to the patient. Additionally, a negative feedback circuit between IL-6 and fatigue intensity was detected. Taken together, this study confirms the necessity of integrating subjective meaning and dynamic complexity into biopsychosocial research in order to understand human functioning under real-life conditions.
A Systematic Review of Long-Interval Intracortical Inhibition as a Biomarker in Neuropsychiatric Disorders
Authors: Fatih Parmis , Kucuker M. Utku , Vande Voort Jennifer L. , Doruk Camsari Deniz , Farzan Faranak , Croarkin Paul E.
Published: June 2021

Long-interval intracortical inhibition (LICI) is a paired-pulse transcranial magnetic stimulation (TMS) paradigm mediated in part by gamma-aminobutyric acid receptor B (GABAB) inhibition. Prior work has examined LICI as a putative biomarker in an array of neuropsychiatric disorders. This review conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) sought to examine existing literature focused on LICI as a biomarker in neuropsychiatric disorders. There were 113 articles that met the inclusion criteria. Existing literature suggests that LICI may have utility as a biomarker of GABAB functioning but more research with increased methodologic rigor is needed. The extant LICI literature has heterogenous methodology and inconsistencies in findings. Existing findings to date are also non-specific to disease. Future research should carefully consider existing methodological weaknesses and implement high-quality test-retest reliability studies.
Perceived Stress During the COVID-19-Related Confinement in Cyprus
Authors: Kyprianidou Maria , Christophi Costas A. , Giannakou Konstantinos
Published: June 2021

The COVID-19 pandemic is a serious global health emergency that could potentially have a significant impact on both somatic as well as psychological level. The aim of this study was to assess the prevalence of perceived stress in the general adult population of Cyprus during the first COVID-19 lockdown period. This was an internet-based cross-sectional study conducted between 6 April and 20 June 2020, one to two and a half months after the introduction of and the first mandatory lockdown on its entire territory imposed by the government of the Republic of Cyprus on 24 March 2020. Data collection was done using a self-administered questionnaire that included information about socioeconomic and demographic characteristics, physical activity, smoking habits, and stress level. A total of 1,485 adults participated in the study. The median perceived stress score was 10 (q1 = 6, q3 = 15). Linear regression models showed that having a medium monthly income (€501-1,500) and being a current smoker was positively associated with the perceived stress score, while being male and physically active was negatively associated with the perceived stress score (all p <0.05). People with medium average salary and current smokers were at a higher risk for perceived stress. Psychological interventions and/or psychological services provided in certain vulnerable groups would be beneficial in future lockdowns due to either COVID-19 or a new pandemic.
Effects of Voluntary and Involuntary Real Lab Situations on Personal Carbon Footprints of Private Households. Experiences From Germany
Authors: Reusswig Fritz , Bock Seraja , Schleer Christoph , Lass Wiebke
Published: June 2021

This paper compares the carbon footprint effects of a voluntary real lab with those of the involuntary “real lab” provided by the lockdowns during the recent Corona crisis. In a voluntary real lab situation, 100 private households in Berlin (Germany) have tried to reduce their personal carbon footprints over 1 year (2018). The households have been equipped with a weekly carbon tracker, calculating their CO2e1 footprint in the domains housing energy, mobility on ground, air travel, food, other consumption, and public consumption. The households have been informed by various channels and supported by a network of “green” local enterprises and NGOs. It was also possible for them to make use of in-home energy advisors, identifying easy-to-accomplish saving potential. On average, the households managed to reduce their footprint by 11%, with individual savings of up to 40%. One of the major problems the households have been experiencing was refraining from air travel, e.g., by substituting it by train l. Others identified road safety as a major problem when switching from cars to bikes. With an annual reduction of 10%, carbon neutrality could be reached in or even before 2050. But political decisions are needed in order to change the collective boundary conditions. In 2020, Germany–as many other countries–has experienced a COVID-19 lockdown with substantial restrictions to all kinds of consumption activities (flying, international travel, consumption activities in the public etc.). This work compares the CO2 reduction effects of these two “real labs,” a voluntary and an imposed one, and ask what the relative quantitative effects are. Following that comparison, it reflect upon the similarities and differences of voluntary and imposed situations, and what there can be learned for the implementation of policies for more sustainable production and consumption patterns.
Toxicity and Femininity in Love Island: How Reality Dating Shows Perpetuate Sexist Attitudes Towards Women
Authors: Denby Alicia
Published: June 2021

Using episodes of ITV2’sLove Island(2016–2020) as a case study, this paper explores the extent to which reality dating shows perpetuate sexist attitudes towards women through a heteronormative focus. Examining the operation of gender roles inLove Island, in the context of emotional intimacy and physical intimacy, this paper proposes that the performance and portrayal of heteronormative ideals disadvantage women. Specifically, by presenting female contestants as overly emotional and irrational, outdated stereotypes surrounding emotionality and hysteria are reproduced withinLove Island. Moreover, the stigmatization of sex-positive women inLove Islanddemonstrates the existence of a sexual double standard wherein male contestants are celebrated for their sexual prowess, while female contestants are shamed and deemed unruly, by virtue of their sexual dominance. Fundamentally, this paper contributes to research on contemporary sexualities by demonstrating how, despite the cultural shift towards greater gender equality, traditional gendered ideals continue to exist in heterosexual relationships, which serve to disadvantage women.
Evaluation of Fed-Batch Fermentation for Production of Polyhydroxybutyrate With a Banana Pulp Juice Substrate From an Agro Industrial By-Product
Authors: Arias-Roblero Mónica , Mora-Villalobos Vanny , Velazquez-Carrillo Carmela
Published: June 2021

Pollution resulting from the persistence of plastics in the environment has driven the development of substitutes for these materials through fermentation processes using agro-industrial wastes. Polyhydroxybutyrate (PHB) is a rapidly biodegradable material with chemical and mechanical properties comparable to those of some petroleum-derived plastics. PHB accumulates intracellularly as an energy reserve in a wide variety of microorganisms exposed to nutritionally imbalanced media. The objective of this study was to evaluate the use of a banana waste product as a carbon source for PHB production. PHB was extracted by acid methanolysis and detected by gas chromatography-mass spectrometry. Eleven bacterial strains with potential for PHB production were evaluated by in vitro fermentation in a culture broth containing fructose as the carbon source and limited nitrogen. A 22 central composite rotational design was applied to optimize the concentrations of banana juice and ammonium chloride needed to maximize the PHB-producing biomass concentration. The process was then carried out in a 3 L fed-batch fermentation system that included an initial stage of biomass growth. Banana juice was used as the carbon source and fructose pulses were added to maintain the test sugar concentrations of 30, 40, and 50 g/L. The control strain, Cupriavidus necator (ATCC 17699), produced 2.816 g/L of PHB, while productivity of the most promising isolate, C. necator (CR-12), was 0.495 g/L. Maximum biomass production was obtained using 5% banana juice and 2 g/L ammonium chloride. PHB production was not detected in fed-batch fermentations supplemented with 30 or 40 g/L of fructose, while the mean PHB production in fermentations with 50 g/L of fructose was 1.3 g/L.
Outcomes and Complications of Bipolar vs. Monopolar Energy for Transurethral Resection of Bladder Tumors: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Mao Xin , Zhou Zhongbao , Cui Yuanshan , Zhang Yong , Yang Mingshan
Published: June 2021

Background:Bipolar and monopolar transurethral resections have a stable status for non-muscle invasive bladder cancer (NMIBC). We conducted a meta-analysis to analyze the outcomes and complications of bipolar vs. monopolar energy for transurethral resection of bladder tumors (TURB).

Methods:The Preferred Reporting Items for Systematic Reviews and Meta-analyses was followed. Based on the Population, Intervention, Comparator, Outcomes, and Study Designs (PICOS) strategy, randomized controlled trials were searched in MEDLINE, EMBASE, and the Cochrane Controlled Trials Register. The reference lists of the associated articles were also retrieved. The data were calculated by Rev Man v5.3.0.

Results:Eleven publications containing an amount of 2, 099 patients were involved in the study. Two groups did not show a significant difference in the mean age and the number of bladder tumors. The results showed that m-TURB had a greater decrease in postoperative hemoglobin level [mean difference (MD) −0.26, 95% confidence interval (CI) −0.48 to −0.04,P= 0.02] and sodium level (MD −0.36, 95% CI −0.62 to −0.10,P= 0.007) compared with b-TURB. B-TURB spent relatively little in hospitalization time (MD −0.52, 95% CI −0.88 to −0.15,P= 0.005) than m-TURB with the exception of operation time (P= 0.47) and catheterization time (P= 0.19). B-TURB did not show a significant difference in the incidence rate of obturator reflex (P= 0.10), bladder perforation (P= 0.32), postoperative blood transfusion (P= 0.28), and clot retention (P= 0.21) compared with the b-TURB group. Besides, there were no significant difference in terms of muscle tissue sampling (P= 0.43), recurrence-free survival at 6 months (P= 0.68) and 12 months (P= 0.78).

Conclusions:B-TURB was more effective than m-TURB in minimizing intraoperative or postoperative bleeding with the smaller loss of hemoglobin and the shorter hospitalization time for patients with NMIBC.
Heritability and Pedigree Analyses of Hypertrophic Cardiomyopathy in Rhesus Macaques (Macaca Mulatta)
Authors: Ueda Yu , Kovacs Samantha , Reader Rachel , Roberts Jeffrey A. , Stern Joshua A.
Published: June 2021

In a colony of rhesus macaques at California National Primate Research Center (CNPRC), naturally occurring hypertrophic cardiomyopathy (HCM) classified by left ventricular hypertrophy without obvious underlying diseases has been identified during necropsy over the last two decades. A preliminary pedigree analysis suggested a strong genetic predisposition of this disease with a founder effect. However, the mode of inheritance was undetermined due to insufficient pedigree data. Since 2015, antemortem examination using echocardiographic examination as well as other cardiovascular analyses have been performed on large numbers of rhesus macaques at the colony. Based on antemortem examination, HCM was diagnosed in additional 65 rhesus macaques. Using HCM cases diagnosed based on antemortem and postmortem examinations, the heritability (h2) was estimated to determine the degree of genetic and environmental contributions to the development of HCM in rhesus macaques at the CNPRC. The calculated mean and median heritability (h2) of HCM in this colony of rhesus macaques were 0.5 and 0.51 (95% confidence interval; 0.14–0.82), respectively. This suggests genetics influence development of HCM in the colony of rhesus macaques. However, post-translational modifications and environmental factors are also likely to contribute the variability of phenotypic expression. Based on the pedigree analysis, an autosomal recessive trait was suspected, but an autosomal dominant mode of inheritance with incomplete penetrance was also possible. Further investigation with more data from siblings, offspring, and parents of HCM-affected rhesus macaques are warranted. Importantly, the findings of the present study support conducting genetic investigations such as whole genome sequencing to identify the causative variants of inherited HCM in rhesus macaques.
Twenty-Four-Hour Ahead Probabilistic Global Horizontal Irradiance Forecasting Using Gaussian Process Regression
Authors: Chandiwana Edina , Sigauke Caston , Bere Alphonce
Published: June 2021

Probabilistic solar power forecasting has been critical in Southern Africa because of major shortages of power due to climatic changes and other factors over the past decade. This paper discusses Gaussian process regression (GPR) coupled with core vector regression for short-term hourly global horizontal irradiance (GHI) forecasting. GPR is a powerful Bayesian non-parametric regression method that works well for small data sets and quantifies the uncertainty in the predictions. The choice of a kernel that characterises the covariance function is a crucial issue in Gaussian process regression. In this study, we adopt the minimum enclosing ball (MEB) technique. The MEB improves the forecasting power of GPR because the smaller the ball is, the shorter the training time, hence performance is robust. Forecasting of real-time data was done on two South African radiometric stations, Stellenbosch University (SUN) in a coastal area of the Western Cape Province, and the University of Venda (UNV) station in the Limpopo Province. Variables were selected using the least absolute shrinkage and selection operator via hierarchical interactions. The Bayesian approach using informative priors was used for parameter estimation. Based on the root mean square error, mean absolute error and percentage bias the results showed that the GPR model gives the most accurate predictions compared to those from gradient boosting and support vector regression models, making this study a useful tool for decision-makers and system operators in power utility companies. The main contribution of this paper is in the use of a GPR model coupled with the core vector methodology which is used in forecasting GHI using South African data. This is the first application of GPR coupled with core vector regression in which the minimum enclosing ball is applied on GHI data, to the best of our knowledge.