bluuebunny/crossref_metadata_2025 huggingface dataset:
E-Cigarette Vapour Increases ACE2 and TMPRSS2 Expression in a Flavour- and Nicotine-Dependent Manner
Authors: Hamon Rhys , Ween Miranda P.
Published: November 2022

COVID-19 infects via the respiratory system, but it can affect multiple systems and lead to multi system failure. There is growing evidence that smoking may be associated with higher rates of COVID-19 infections and worse outcomes due to increased levels of ACE2 in lung epithelial cells, but it is unknown whether E-cigarette use may lead to increased risk of COVID-19 infection from the SARS-CoV-2 virus. In this study, healthy donor bronchial epithelial cells (NHBE) and monocyte-derived macrophages (MDM) were exposed to cigarette smoke extract (CSE) or nicotine or flavoured E-cigarette vapour extract (EVE) before the assessment of SARS-CoV-2 recognition receptors ACE2 and TMPRSS2 genes. MDMs exposed to CSE and Tobacco EVE showed increased ACE2 expression; however, no treatment altered the TMPRSS2 expression. ACE2 was found to be upregulated by >2-fold in NHBE cells exposed to CSE, as well as nicotine, banana, or chocolate EVE, while TMPRSS2 was only upregulated by CSE or nicotine EVE exposure. These findings suggesting that flavourings can increase ACE2 expression in multiple cell types, while TMPRSS2 expression increases are limited to the epithelial cells in airways and may be limited to nicotine and/or cigarette smoke exposure. Therefore, increased risk of COVID-19 infection cannot be ruled out for vapers.
Effects of Different Acute Plyometric Training Intensities on Attention and Psychological States
Authors: Znazen Hela , Hammami Amri , Bragazzi Nicola Luigi , Hadadi Atyh , Slimani Maamer
Published: November 2022

The objective of this investigation was to explore in a sample of female students the effects of several acute plyometric training intensities (low, moderate, and high—55–65%, 70–80%, and 90–100% of maximal vertical jump performance, respectively) on cognition (attention) and psychological states (mood). Thirty-seven female students (mean age = 19.72 ± 0.73 years, mean body mass index = 19.51) participated in the current study. They were randomly allocated to one of three conditions: a high-intensity plyometric exercise (HIPE), a moderate-intensity plyometric exercise (MIPE), and a low-intensity plyometric exercise (LIPE). Before and immediately after each session for the three conditions, all participants underwent a cognitive performance test (d2 test) and filled in a battery of psychological questionnaires (the Rating of Perceived Exertion (RPE), and the Brunel Mood Scale (BRUMS)). The data reported higher concentration performance and a lower number of errors in the MIPE when compared with HIPE (all, p-value < 0.05) groups, whereas no significant difference was found between other conditions (p-value > 0.05). The RPE value was higher in the HIPE (p-value < 0.001) and MIPE (p = 0.01) than in the LIPE, and in the HIPE than in the MIPE (p = 0.001) conditions. Concerning the BRUMS scale, fatigue (p = 0.005) was significantly different among the various conditions, being higher in HIPE with respect to MIPE and LIPE (all, p-value < 0.05) conditions. In conclusion, moderate-intensity plyometric exercise can be considered the best activity to improve visual attention. Practitioners may practice moderate-intensity plyometric exercises to improve concentration performance. However, due to the limitations of the present study (lack of a control group and between-subjects study design), further research in the field is warranted.
Using Random Effect Models to Produce Robust Estimates of Death Rates in COVID-19 Data
Authors: Almohaimeed Amani , Einbeck Jochen , Qarmalah Najla , Alkhidhr Hanan
Published: November 2022

Tracking the progress of an infectious disease is critical during a pandemic. However, the incubation period, diagnosis, and treatment most often cause uncertainties in the reporting of both cases and deaths, leading in turn to unreliable death rates. Moreover, even if the reported counts were accurate, the “crude” estimates of death rates which simply divide country-wise reported deaths by case numbers may still be poor or even non-computable in the presence of small (or zero) counts. We present a novel methodological contribution which describes the problem of analyzing COVID-19 data by two nested Poisson models: (i) an “upper model” for the cases infected by COVID-19 with an offset of population size, and (ii) a “lower” model for deaths of COVID-19 with the cases infected by COVID-19 as an offset, each equipped with their own random effect. This approach generates robustness in both the numerator as well as the denominator of the estimated death rates to the presence of small or zero counts, by “borrowing” information from other countries in the overall dataset, and guarantees positivity of both the numerator and denominator. The estimation will be carried out through non-parametric maximum likelihood which approximates the random effect distribution through a discrete mixture. An added advantage of this approach is that it allows for the detection of latent subpopulations or subgroups of countries sharing similar behavior in terms of their death rates.
Risk Assessment of Heavy Metal Pollution in Farmland Soils at the Northern Foot of the Qinling Mountains, China
Authors: Huo Aidi , Wang Xing , Zhao Zhixin , Yang Luying , Zhong Fangqian , Zheng Chunli , Gao Ningbo
Published: November 2022

To provide scientific basis for the prevention and control of heavy metal pollution, a field investigation, sample collection and analysis of the heavy metal content in farmland soils at the northern foot of the Qinling Mountains were conducted. Based on the comparative analysis of the single pollution index method, the Nemerow comprehensive pollution index method, the geological accumulation index method, the potential ecological hazard index method, and the geological accumulation index method were used to comprehensively analyze and evaluate the risk of soil heavy metal pollution. The results showed that the heavy metal pollution of farmland soil at the northern foot of the Qinling Mountains was severe, among which Hg and Cr pollution was relatively obvious. Taking the soil screening values of agricultural land as the standard, the quantity of element Hg in agricultural soils at the northern foot of the Qinling Mountains was higher than the relevant screening value. In the two sample sites investigated, the intensity of the heavy metal accumulation index in Baoqizhai Village was Hg > Cr > Cu > As > Pb, and in Dayangyu Village it was Cr > Cu > As > Pb. Among them, in Baoqizhai Village it shows the heavy pollution caused by Hg (Igeo= 3.42) and the light pollution caused by Cr (Igeo < 1) in the two areas. Hg is mostly affected by mining activities and its atmospheric subsidence. At the same time, Cr is mainly derived from the weathering of rock parent material and is also affected by anthropogenic factors to a certain extent. The accumulation of heavy metals in the farmland soil around the northern foot of the Qinling Mountains was relatively high, posing a threat to the surrounding soil environment. Therefore, it is urgent to control farmland soil environmental pollution.
Lead Responses and Tolerance Mechanisms of Koelreuteria paniculata: A Newly Potential Plant for Sustainable Phytoremediation of Pb-Contaminated Soil
Authors: Su Rongkui , Xie Tianzhi , Yao Haisong , Chen Yonghua , Wang Hanqing , Dai Xiangrong , Wang Yangyang , Shi Lei , Luo Yiting
Published: November 2022

Phytoremediation could be an alternative strategy for lead (Pb) contamination. K. paniculata has been reported as a newly potential plant for sustainable phytoremediation of Pb-contaminated soil. Physiological indexes, enrichment accumulation characteristics, Pb subcellular distribution and microstructure of K. paniculata were carefully studied at different levels of Pb stress (0–1200 mg/L). The results showed that plant growth increased up to 123.8% and 112.7%, relative to the control group when Pb stress was 200 mg/L and 400 mg/L, respectively. However, the average height and biomass of K. paniculata decrease when the Pb stress continues to increase. In all treatment groups, the accumulation of Pb in plant organs showed a trend of root > stem > leaf, and Pb accumulation reached 81.31%~86.69% in the root. Chlorophyll content and chlorophyll a/b showed a rising trend and then fell with increasing Pb stress. Catalase (CAT) and peroxidase (POD) activity showed a positive trend followed by a negative decline, while superoxide dismutase (SOD) activity significantly increased with increasing levels of Pb exposure stress. Transmission electron microscopy (TEM) showed that Pb accumulates in the inactive metabolic regions (cell walls and vesicles) in roots and stems, which may be the main mechanism for plants to reduce Pb biotoxicity. Fourier transform infrared spectroscopy (FTIR) showed that Pb stress increased the content of intracellular -OH and -COOH functional groups. Through organic acids, polysaccharides, proteins and other compounds bound to Pb, the adaptation and tolerance of K. paniculata to Pb were enhanced. K. paniculata showed good phytoremediation potential and has broad application prospects for heavy metal-contaminated soil.
The Associations between 24-Hour Movement Behaviours and Quality of Life in Preschoolers: A Compositional Analysis of Cross-Sectional Data from 2018–2021
Authors: Chen Meiyuan , Chua Terence , Shen Zhi , Tay Lee Yong , Wang Xiaozan , Chia Michael
Published: November 2022

Studies show that the quality of life (QoL) of preschoolers is closely related to physical activity (PA), sedentary behaviour (SB), and sleep (SL). Yet many researchers looked at these 24-h movement behaviours as behaviours that are independent of one another when examining the association of QoL with these behaviours. The main purpose of the present study was to describe the temporal trends in PA, SB, and SL in preschoolers and the concomitant association with QoL of children. Annual cross-sectional data on QoL and 24-h movement behaviours of 8045 Singaporean preschoolers were collected from 2018 to 2021. Compositional analysis, linear regression, and isotemporal replacement approaches were used to examine changes in PA, SB, and SL from 2018–2021 and how these changes were associated with QoL. Temporal trends in movement behaviours showed that PA and SL decreased after 2020. During 2018–2021, the association of PA and QoL in preschoolers was characterised by a ‘U’ curve (βPA-2018 = 3.06, p < 0.001; βPA-2019 = 1.43, p < 0.05; βPA-2020 = −0.43, p > 0.05; βPA-2021 = 2.82, p < 0.001), while SL and QoL were characterised by an inverted ‘U’ curve (βSL-2018 = −2.39, p < 0.001; βSL-2019 = −0.27, p > 0.05; βSL-2020 = 2.00, p < 0.01; βSL-2021 = −0.21, p > 0.05). SB was significantly and negatively associated with QoL after 2020, with 2020 identified as the inflection point for the change in SB (βSB-2018 = 0.67, p > 0.05; βSB-2019 = −1.16, p > 0.05; βSB-2020 = −1.56, p < 0.01; βSB-2021 = −2.61, p < 0.01). Using a time re-allocation technique to treat the 2021 data, reallocating time from SB to PA or to SL predicted improvements in QoL of preschoolers (Pall < 0.05). The study provided useful information on the temporal trends in PA, SB, SL, and QoL of preschoolers over four years. Additionally, these data provided insights into how changes in QoL are predicted by changes in duration in the 24-h movement behaviours.
Comparison and Functional Analysis of Odorant-Binding Proteins and Chemosensory Proteins in Two Closely Related Thrips Species, Frankliniella occidentalis and Frankliniella intonsa (Thysanoptera: Thripidae) Based on Antennal Transcriptome Analysis
Authors: Li Xiaowei , Cheng Jianghui , Chen Limin , Huang Jun , Zhang Zhijun , Zhang Jinming , Ren Xiaoyun , Hafeez Muhammad , Zhou Shuxing , Dong Wanying , Lu Yaobin
Published: November 2022

Two closely related thrips species, Frankliniella occidentalis and Frankliniella intonsa, are important pests on agricultural and horticultural crops. They have several similarities, including occurrence patterns, host range, and aggregation pheromone compounds. However, there are very few reports about the chemosensory genes and olfactory mechanisms in these two species. To expand our knowledge of the thrips chemosensory system, we conducted antennal transcriptome analysis of two thrips species, and identified seven odorant-binding proteins (OBPs) and eight chemosensory proteins (CSPs) in F. occidentalis, as well as six OBPs and six CSPs in F. intonsa. OBPs and CSPs showed high sequence identity between the two thrips species. The RT-qPCR results showed that the orthologous genes FoccOBP1/3/4/5/6, FintOBP1/3/4/6, FoccCSP1/2/3, and FintCSP1/2 were highly expressed in male adults. Molecular docking results suggested that orthologous pairs FoccOBP4/FintOBP4, FoccOBP6/FintOBP6, and FoccCSP2/FintCSP2 might be involved in transporting the major aggregation pheromone compound neryl (S)-2-methylbutanoate, while orthologous pairs FoccOBP6/FintOBP6, FoccCSP2/FintCSP2, and FoccCSP3/FintCSP3 might be involved in transporting the minor aggregation pheromone compound (R)-lavandulyl acetate. These results will provide a fundamental basis for understanding the molecular mechanisms of pheromone reception in the two thrips species.
Development of Radiosterilized Porcine Skin Electrosprayed with Silver Nanoparticles Prevents Infections in Deep Burns
Authors: Pérez-Díaz Mario Alberto , Alvarado-Gómez Elizabeth , Martínez-Pardo María Esther , José Yacamán Miguel , Flores-Santos Andrés , Sánchez-Sánchez Roberto , Martínez-Gutiérrez Fidel , Bach Horacio
Published: November 2022

Extensive burns represent a significant challenge in biomedicine due to the multiple systemic and localized complications resulting from the major skin barrier loss. The functionalization of xenografts with nanostructured antibacterial agents proposes a fast and accessible application to restore barrier function and prevent localized bacterial contamination. Based on this, the objective of this work was to functionalize a xenograft by electrospray deposition with silver nanoparticles (AgNPs) and to evaluate its antibiofilm and cytotoxic effects on human fibroblasts. Initially, AgNPs were synthesized by a green microwave route with sizes of 2.1, 6.8, and 12.2 nm and concentrations of 0.055, 0.167, and 0.500 M, respectively. The AgNPs showed a size relationship directly proportional to the concentration of AgNO3, with a spherical and homogeneous distribution determined by high-resolution transmission electron microscopy. The surface functionalization of radiosterilized porcine skin (RPS) via electrospray deposition with the three AgNP concentrations (0.055, 0.167, and 0.500 M) in the epidermis and the dermis showed a uniform distribution on both surfaces by energy-dispersive X-ray spectroscopy. The antibiofilm assays of clinical multidrug-resistant Pseudomonas aeruginosa showed significant effects at the concentrations of 0.167 and 0.500 M, with a log reduction of 1.3 and 2.6, respectively. Additionally, viability experiments with human dermal fibroblasts (HDF) exposed to AgNPs released from functionalized porcine skin showed favorable tolerance, with retention of viability more significant than 90% for concentrations of 0.05 and 0.167 M after 24 h exposure. Antibacterial activity combined with excellent biocompatibility makes this biomaterial a candidate for antibacterial protection by inhibiting bacterial biofilms in deep burns during early stages of development.
Alzheimer’s Disease: Treatment Strategies and Their Limitations
Authors: Passeri Elodie , Elkhoury Kamil , Morsink Margaretha , Broersen Kerensa , Linder Michel , Tamayol Ali , Malaplate Catherine , Yen Frances T. , Arab-Tehrany Elmira
Published: November 2022

Alzheimer’s disease (AD) is the most frequent case of neurodegenerative disease and is becoming a major public health problem all over the world. Many therapeutic strategies have been explored for several decades; however, there is still no curative treatment, and the priority remains prevention. In this review, we present an update on the clinical and physiological phase of the AD spectrum, modifiable and non-modifiable risk factors for AD treatment with a focus on prevention strategies, then research models used in AD, followed by a discussion of treatment limitations. The prevention methods can significantly slow AD evolution and are currently the best strategy possible before the advanced stages of the disease. Indeed, current drug treatments have only symptomatic effects, and disease-modifying treatments are not yet available. Drug delivery to the central nervous system remains a complex process and represents a challenge for developing therapeutic and preventive strategies. Studies are underway to test new techniques to facilitate the bioavailability of molecules to the brain. After a deep study of the literature, we find the use of soft nanoparticles, in particular nanoliposomes and exosomes, as an innovative approach for preventive and therapeutic strategies in reducing the risk of AD and solving problems of brain bioavailability. Studies show the promising role of nanoliposomes and exosomes as smart drug delivery systems able to penetrate the blood–brain barrier and target brain tissues. Finally, the different drug administration techniques for neurological disorders are discussed. One of the promising therapeutic methods is the intranasal administration strategy which should be used for preclinical and clinical studies of neurodegenerative diseases.
Peripheral Gonadotropin-Inhibitory Hormone (GnIH) Acting as a Novel Modulator Involved in Hyperphagia-Induced Obesity and Associated Disorders of Metabolism in an In Vivo Female Piglet Model
Authors: Chen Lei , Zhang Xin , Song Xingxing , Han Dongyang , Han Kaiou , Xu Wenhao , Luo Rongrong , Cao Yajie , Shi Yan , Liu Chengcheng , Xu Changlin , Li Zixin , Li Yinan , Li Xun
Published: November 2022

Apart from the well-established role of the gonadotropin-inhibitory hormone (GnIH) in the regulation of the reproductive functions, much less is known about the peripheral role of the GnIH and its receptor in the metabolic processes. On account of pig being an excellent model for studies of food intake and obesity in humans, we investigated the peripheral effects of the GnIH on food intake and energy homeostasis and revealed the underlying mechanism(s) in female piglets in vivo. Compared to the vehicle-treated group, intraperitoneally injected GnIH significantly increased the food intake and altered the meal microstructure both in the fasting and ad libitum female piglet. GnIH-triggered hyperphagia induced female piglet obesity and altered islet hormone secretion in the pancreas, accompanied with dyslipidemia and hyperglycemia. Interestingly, GnIH decreased the glucose transport capacity and glycogen synthesis, whereas it increased the gluconeogenesis in the liver, while it also induced an insulin resistance in white adipose tissue (WAT) via inhibiting the activity of AKT-GSK3-β signaling. In terms of the lipid metabolism, GnIH reduced the oxidation of fatty acids, whereas the elevated fat synthesis ability in the liver and WAT was developed though the inhibited AMPK phosphorylation. Our findings demonstrate that peripheral GnIH could trigger hyperphagia-induced obesity and an associated glycolipid metabolism disorder in female piglets, suggesting that GnIH may act as a potential therapeutic agent for metabolic syndrome, obesity and diabetes.
Genome-Wide Identification of the Aconitase Gene Family in Tomato (Solanum lycopersicum) and CRISPR-Based Functional Characterization of SlACO2 on Male-Sterility
Authors: Secgin Zafer , Uluisik Selman , Yıldırım Kubilay , Abdulla Mohamed Farah , Mostafa Karam , Kavas Musa
Published: November 2022

Tomato (Solanum lycopersicum) is one of the most cultivated vegetables in the world due to its consumption in a large variety of raw, cooked, or processed foods. Tomato breeding and productivity highly depend on the use of hybrid seeds and their higher yield, environmental adaption, and disease tolerance. However, the emasculation procedure during hybridization raises tomato seed production costs and labor expenses. Using male sterility is an effective way to reduce the cost of hybrid seeds and ensure cultivar purity. Recent developments in CRISPR genome editing technology enabled tomato breeders to investigate the male sterility genes and to develop male-sterile tomato lines. In the current study, the tomato Acotinase (SlACO) gene family was investigated via in silico tools and functionally characterized with CRISPR/Cas9-mediated gene disruption. Genome-wide blast and HMM search represented two SlACO genes located on different tomato chromosomes. Both genes were estimated to have a segmental duplication in the tomato genome due to their identical motif and domain structure. One of these genes, SlACO2, showed a high expression profile in all generative cells of tomato. Therefore, the SlACO2 gene was targeted with two different gRNA/Cas9 constructs to identify their functional role in tomatoes. The gene was mutated in a total of six genome-edited tomato lines, two of which were homozygous. Surprisingly, pollen viability was found to be extremely low in mutant plants compared to their wild-type (WT) counterparts. Likewise, the number of seeds per fruit also sharply decreased more than fivefold in mutant lines (10–12 seeds) compared to that in WT (67 seeds). The pollen shape, anther structures, and flower colors/shapes were not significantly varied between the mutant and WT tomatoes. The mutated lines were also subjected to salt and mannitol-mediated drought stress to test the effect of SlACO2 on abiotic stress tolerance. The results of the study indicated that mutant tomatoes have higher tolerance with significantly lower MDA content under stress conditions. This is the first CRISPR-mediated characterization of ACO genes on pollen viability, seed formation, and abiotic stress tolerance in tomatoes.
Brain-Derived Neurotrophic Factor (BDNF) Is Associated with Platelet Activity and Bleeding Tendency in Patients with Gaucher Disease
Authors: Azoulay David , Naamad Mira , Frydman Dafna , Broide Ellen , Zimran Ari , Stemer Galia , Revel-Vilk Shoshana
Published: November 2022

Bleeding tendency, a prominent feature of patients with Gaucher disease (GD), is associated with abnormal platelet function. Brain-derived neurotrophic factor (BDNF) is a protein with neuroprotective potential stored in alpha granules of circulating platelets. Here we studied BDNF levels in 50 patients with type I GD (GD1) and their correlation with platelet activity and bleeding tendency. Flow cytometry was used to test unstimulated and stimulated measurement of platelet surface-activated expression of αIIbβ3 integrin, P-selectin and lysosomal-associated membrane protein (LAMP3/CD63). Serum and plasma BDNF levels were quantified using ELISA. The bleeding history was recorded by a bleeding questionnaire. Serum BDNF levels were positively correlated with platelet count and moderately correlated with unstimulated and stimulated platelet P-selectin expression. Patients with more than one bleeding manifestation were shown to have lower serum BDNF levels, albeit similar platelet count. Plasma BDNF levels were significantly elevated in splenectomized patients and showed a moderate positive correlation with stimulated platelet CD63 expression. These observations demonstrate the first association between BDNF levels in the peripheral blood with platelet dysfunction and increased bleeding manifestation. The role of measuring serum BDNF for assessing platelet alpha degranulation defects and bleeding risk in patients with GD and the general population needs further study.
Human Vascular Endothelial Cells Promote the Secretion of Vascularization Factors and Migration of Human Skin Fibroblasts under Co-Culture and Its Preliminary Application
Authors: Hou Tian , Du Miaomiao , Gao Xiang , An Meiwen
Published: November 2022

The good treatment of skin defects has always been a challenge in the medical field, and the emergence of tissue engineering skin provides a new idea for the treatment of injured skin. However, due to the single seed cells, the tissue engineering skin has the problem of slow vascularization at the premonitory site after implantation into the human body. Cell co-culture technology can better simulate the survival and communication environment of cells in the human body. The study of multicellular co-culture hopes to bring a solution to the problem of tissue engineering. In this paper, human skin fibroblasts (HSFs) and human vascular endothelial cells (HVECs) were co-cultured in Transwell. The Cell Counting Kit 8 (CCK8), Transwell migration chamber, immunofluorescence, Western blot (WB), and real time quantitative PCR (RT-qPCR) were used to study the effects of HVECs on cell activity, migration factor (high mobility group protein 1, HMGB1) and vascularization factor (vascular endothelial growth factor A, VEGFA and fibroblast growth factor 2, FGF2) secretion of HSFs after co-cultured with HVECs in the Transwell. The biological behavior of HSFs co-cultured with HVECs was studied. The experimental results are as follows: (1) The results of cck8 showed that HVECS could promote the activity of HSFs. (2) HVECs could significantly promote the migration of HSFs and promote the secretion of HMGB1. (3) HVECs could promote the secretion of VEGFA and FGF2 of HSFs. (4) The HVECs and HSFs were inoculated on tissue engineering scaffolds at the ratio of 1:4 and were co-cultured and detected for 7 days. The results showed that from the third day, the number of HSFs was significantly higher than that of the control group without HVECs.
Signaling Pathways in Inflammation and Cardiovascular Diseases: An Update of Therapeutic Strategies
Authors: Cucu Ioana
Published: November 2022

Inflammatory processes represent a pivotal element in the development and complications of cardiovascular diseases (CVDs). Targeting these processes can lead to the alleviation of cardiomyocyte (CM) injury and the increase of reparative mechanisms. Loss of CMs from inflammation-associated cardiac diseases often results in heart failure (HF). Evidence of the crosstalk between nuclear factor-kappa B (NF-κB), Hippo, and mechanistic/mammalian target of rapamycin (mTOR) has been reported in manifold immune responses and cardiac pathologies. Since these signaling cascades regulate a broad array of biological tasks in diverse cell types, their misregulation is responsible for the pathogenesis of many cardiac and vascular disorders, including cardiomyopathies and atherosclerosis. In response to a myriad of proinflammatory cytokines, which induce reactive oxygen species (ROS) production, several molecular mechanisms are activated within the heart to inaugurate the structural remodeling of the organ. This review provides a global landscape of intricate protein–protein interaction (PPI) networks between key constituents of NF-κB, Hippo, and mTOR signaling pathways as quintessential targetable candidates for the therapy of cardiovascular and inflammation-related diseases.
Reference Genes for Expression Analysis Using RT-qPCR in Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)
Authors: Zhao Xiaoyu , Guo Jiawen , Lu Yanhui , Sun Tianyi , Tian Junce , Huang Jianlei , Xu Hongxing , Wang Zhengliang , Lu Zhongxian
Published: November 2022

Cnaphalocrocis medinalis is a destructive migratory rice pest. Although many studies have investigated its behavioral and physiological responses to environmental changes and migration-inducing factors, little is known about its molecular mechanisms. This study was conducted to select suitable RT-qPCR reference genes to facilitate future gene expression studies. Here, thirteen candidate housekeeping genes (EF1α, AK, EF1β, GAPDH, PGK, RPL13, RPL18, RPS3, 18S rRNA, TBP1, TBP2, ACT, and UCCR) were selected to evaluate their stabilities under different conditions using the ∆CT method; the geNorm, NormFinder, BestKeeper algorithms; and the online tool RefFinder. The results showed that the most stable reference genes were EF1β, PGK, and RPL18, related to developmental stages; RPS3 and RPL18 in larval tissues; EF1β and PGK in larvae feeding on different rice varieties; EF1α, EF1β, and PGK in larvae temperature treatments; PGK and RPL13, related to different adult ages; PGK, EF1α, and ACT, related to adult nutritional conditions; RPL18 and PGK, related to adult mating status; and, RPS3 and PGK, related to different adult take-off characteristics. Our results reveal reference genes that apply to various experimental conditions and will greatly improve the reliability of RT-qPCR analysis for the further study of gene function in this pest.
Xuebijing Administration Alleviates Pulmonary Endothelial Inflammation and Coagulation Dysregulation in the Early Phase of Sepsis in Rats
Authors: Lv Jie , Guo Xiaoxia , Zhao Huiying , Zhou Gang , An Youzhong
Published: November 2022

Ethnopharmacological relevance: Xuebijing injection is a Chinese herbal-derived drug composed of radix paeoniaerubra, rhizomachuanxiong, Salvia miltiorrhiza, floscarthami, and Angelica sinensis. This study aimed to investigate the effects of Xuebijing administration on pulmonary endothelial injury and coagulation dysfunction in a cecal ligation and puncture (CLP)-induced sepsis rat model. Materials and methods: A CLP-induced sepsis rat model was established. The CLP rats were treated with a vehicle or Xuebijing via intravenous infusion and sacrificed at 2, 4, 6, 8, or 12 h after CLP for lung tissue and blood sample collection. The mean arterial pressure (MAP) was monitored. Transmission microscopy examination and H&E staining were performed to observe pulmonary structural alterations. Enzyme linked immunosorbent assay (ELISA) was performed to measure the plasma levels of epithelial markers, proinflammatory cytokines, and coagulation-related proteins. Results: Compared with vehicle treatment, Xuebijing administration maintained the MAP in the normal range until 11 h after CLP. Transmission microscopy and H&E staining revealed that Xuebijing administration alleviated alveolar–capillary barrier impairments and lung inflammation in CLP rats. ELISA showed that Xuebijing administration effectively reversed CLP-induced elevations in the plasma levels of epithelial markers endothelin-1 and von Willebrand factor, starting 6 and 8 h after CLP, respectively. Xuebijing administration also significantly abolished CLP-induced rises in circulating proinflammatory cytokines interleukin 6 (IL-6) at 6 h after CLP, IL-1β at 2 and 12 h after CLP, and TNF-α at 2, 4, 6, 8, and 12 h after CLP. In addition, Xuebijing administration strongly reversed CLP-induced alterations in circulating active protein C and tissue-type plasminogen activator, starting 4 h and 2 h after CLP, respectively. Conclusions: Xuebijing ameliorates pulmonary endothelial injury, systemic inflammation, and coagulation dysfunction in early sepsis.
Evaluation and Catheter Ablation of Ventricular Arrhythmias in Cardiac Sarcoidosis
Authors: Ezzeddine Fatima M. , Tan Nicholas , Siontis Konstantinos C.
Published: November 2022

Ventricular arrhythmias are a common clinical manifestation in patients with cardiac sarcoidosis (CS) and other arrhythmogenic inflammatory cardiomyopathies (AIC). The management of sustained ventricular arrhythmias in these patients presents unique challenges. Current therapies include immunosuppressive, antiarrhythmic agents, and catheter ablation. Significant progress has been made in deciphering the importance of patient selection for ablation, systematic preablation evaluation, and optimal ablation timing, as well as ablation approaches and techniques. In this overview, we discuss the evaluation and management of ventricular arrhythmias in patients with CS, focusing on catheter ablation, which has evolved into an effective approach in reducing the burden of ventricular arrhythmias in these patients in the context of multifaceted treatment along with medical therapies.
Hemodynamic Analysis of the Geometric Features of Side Holes Based on GDK Catheter
Authors: Yang Yang , Li Yijing , Liu Chen , Zhou Jingyuan , Li Tao , Xiong Yan , Zhang Ling
Published: November 2022

Hemodialysis is an important means to maintain life in patients with end-stage renal disease (ESRD). Approximately 76.8% of patients who begin hemodialysis do so through catheters, which play vital roles in the delivery of hemodialysis to patients. During the past decade, the materials, structures, and surface-coating technologies of catheters have constantly been evolving to ameliorate catheter-related problems, such as recirculation, thrombosis, catheter-related infections, and malfunction. In this study, based on the commercial GDK catheter, six catheter models (GDK, GDK1, GDK2, GDK3, GDK4, and GDK5) with different lumen diameters and different geometric features of side holes were established, and computational flow dynamics (CFD) were used to measure flow rate, shear stress, residence time (RT), and platelet lysis index (PLI). These six catheters were then printed with polycarbonate PC using 3D printing technology to verify recirculation rates. The results indicated that: (1) the catheter with a 5.5 mm outer diameter had the smallest average shear stress in the arterial lumen and the smallest proportion of areas with shear stress > 10 pa. With increasing catheter diameter, the shear stress in the tip volume became lower, the average RT increased, and the PLI decreased due to larger changes in shear stress; (2) the catheters with oval-shaped side holes had smaller shear stress levels than those with circular-shaped holes, indicating that the oval design was more effective; (3) the catheter with parallel dual side holes had uniformly distributed flow around side holes and exhibited lower recirculation rates in both forward and reverse connections, while linear multi-side holes had higher shear stress levels due to the large differences in flow around side holes. The selection of the material and the optimization of the side holes of catheters have significant impacts on hemodynamic performances and reduce the probability of thrombosis, thus improving the efficiency of dialysis. This study would provide some guidance for optimizing catheter structures and help toward the commercialization of more efficient HD catheters.
A New Zn(II) Metal Hybrid Material of 5-Nitrobenzimidazolium Organic Cation (C7H6N3O2)2[ZnCl4]: Elaboration, Structure, Hirshfeld Surface, Spectroscopic, Molecular Docking Analysis, Electric and Dielectric Properties
Authors: Ayari Chaima , Alotaibi Abdullah A. , Baashen Mohammed A. , Perveen Fouzia , Almarri Abdulhadi H. , Alotaibi Khalid M. , Abdelbaky Mohammed S. M. , Garcia-Granda Santiago , Othmani Abdelhak , Nasr Cherif Ben , Mrad Mohamed Habib
Published: November 2022

The slow solvent evaporation approach was used to create a single crystal of (C7H6N3O2)2[ZnCl4] at room temperature. Our compound has been investigated by single-crystal XRD which declares that the complex crystallizes in the monoclinic crystallographic system with the P21/c as a space group. The molecular arrangement of the compound can be described by slightly distorted tetrahedral ZnCl42− anionic entities and 5-nitrobenzimidazolium as cations, linked together by different non-covalent interaction types (H-bonds, Cl…Cl, π…π and C–H…π). Hirshfeld’s surface study allows us to identify that the dominant contacts in the crystal building are H…Cl/Cl…H contacts (37.3%). FT-IR method was used to identify the different groups in (C7H6N3O2)2[ZnCl4]. Furthermore, impedance spectroscopy analysis in 393 ≤ T ≤ 438 K shows that the temperature dependence of DC conductivity follows Arrhenius’ law. The frequency–temperature dependence of AC conductivity for the studied sample shows one region (Ea = 2.75 eV). In order to determine modes of interactions of compound with double stranded DNA, molecular docking simulations were performed at molecular level.
Study on the Work Hardening and Metamorphic Layer Characteristics of Milling Contour Bevel Gears
Authors: Wu Mingyang , Zhang Jianyu , Ma Chunjie , Zhang Yali , Cheng Yaonan , Wu Shi , Li Lubin
Published: November 2022

High temperature and strain will occur in the cutting area during dry milling of contour bevel gears, which causes plastic deformation of the workpiece, resulting in changes in the physical properties of the machined surface’s metamorphic layer, reducing the quality of the workpiece’s machined surface. Therefore, it is necessary to investigate the properties of the metamorphic layer and the work hardening behavior of the machined surfaces of contour bevel gears. The paper first establishes a single-tooth finite element simulation model for a contour bevel gear and extracts the temperature field, strain field and strain rate at different depths from the machined surface. Then, based on the simulation results, the experiment of milling contour bevel gears is carried out, the microscopic properties of the machined metamorphic layer are studied using XRD diffractometer and ultra-deep field microscopy, and the work hardening behavior of the machined metamorphic layer under different cutting parameters is studied. Finally, the influence of the cutting parameters on the thickness of the metamorphic layer of the machined surface is investigated by scanning electron microscopy. The research results can not only improve the surface quality and machinability of the workpiece, but are also significant for increasing the fatigue strength of the workpiece.
Formation of Nanoscale Al2O3 Protective Layer by Preheating Treatment for Improving Corrosion Resistance of Dilute Fe-Al Alloys
Authors: Li Chenglong , Freiberg Katharina , Tang Yuntong , Lippmann Stephanie , Zhu Yongfu
Published: November 2022

In this work, an attempt was made to improve the corrosion resistance of dilute Fe-Al alloys (1.0 mass% Al) by preheating treatment at 1073 K in H2 atmosphere. In comparison with pure Fe and unpreheated Fe-Al alloys, the resistance to oxidation at 673 K in pure O2 and to electrochemical corrosion in 5 wt.% NaCl solution is significantly improved for preheated Fe-Al alloys. This improvement is attributed to the formation of a 20 nm thin, but dense Al2O3 protective layer on the surface of preheated Fe-Al alloys.
The Effect of Initial Grain Size on the Nanocrystallization of AZ31 Mg Alloy during Rotary Swaging
Authors: Chen Xin , Li Silong , Wan Yingchun
Published: November 2022

Nanograins were obtained in the AZ31 Mg alloy bars with different initial grain sizes via cold rotary swaging. Microstructure evolution during deformation was investigated through electron backscatter diffraction analysis and transmission electron microscopy studies. The results indicate that initial grain size had little effect on the mechanism of grain refinement during swaging. The nanocrystallization process of the alloys with different initial grain sizes included extensive twinning followed by the further refinement of the twin lamellae through the formation of massive dislocation arrays. However, as the initial grain size decreased, the formation rate of nanograins increased, resulting in a higher degree of nanocrystallization after the same swaging pass. The mean grain size and yield strength of the sample with the smallest initial grain size were about 91 nm and 489 MPa, respectively. The slower rate and lower degree of nanocrystallization in the alloy with a larger initial grain size were mainly attributed to the less grain boundary areas and higher activity of twinning.
Strength and Durability of Sustainable Self-Consolidating Concrete with High Levels of Supplementary Cementitious Materials
Authors: Salih Moslih Amer , Ahmed Shamil Kamil , Alsafi Shaymaa , Abullah Mohd Mustafa Al Bakri , Jaya Ramadhansyah Putra , Abd Rahim Shayfull Zamree , Aziz Ikmal Hakem , Thanaya I Nyoman Arya
Published: November 2022

Self-consolidating concrete (SCC) has been used extensively in the construction industry because of its advanced characteristics of a highly flowable mixture and the ability to be consolidated under its own weight. One of the main challenges is the high content of OPC used in the production process. This research focuses on developing sustainable, high-strength self-consolidating concrete (SCC) by incorporating high levels of supplementary cementitious materials. The overarching purpose of this study is to replace OPC partially by up to 71% by using fly ash, GGBS, and microsilica to produce high-strength and durable SCC. Two groups of mixtures were designed to replace OPC. The first group contained 14%, 23.4%, and 32.77% fly ash and 6.4% microsilica. The second group contained 32.77%, 46.81%, and 65.5% GGBS and 6.4% microsilica. The fresh properties were investigated using the slump, V-funnel, L-box, and J-ring tests. The hardened properties were assessed using a compressive strength test, while water permeability, water absorption, and rapid chloride penetration tests were used to evaluate the durability. The innovation of this experimental work was introducing SCC with an unconventional mixture that can achieve highly durable and high-strength concrete. The results showed the feasibility of SCC by incorporating high volumes of fly ash and GGBS without compromising compressive strength and durability.
Study of Wear, Stress and Vibration Characteristics of Silicon Carbide Tool Inserts and Nano Multi-Layered Titanium Nitride-Coated Cutting Tool Inserts in Turning of SS304 Steels
Authors: Ganeshkumar S. , Singh Bipin Kumar , Kumar S. Dharani , Gokulkumar S. , Sharma Shubham , Mausam Kuwar , Li Changhe , Zhang Yanbin , Tag Eldin Elsayed Mohamed
Published: November 2022

Cutting tool characterization plays a crucial role in understanding the behavior of machining operations. The selection of a suitable cutting material, the operating conditions for the work piece, is necessary to yield good cutting-tool life. Several pieces of research have been carried out in cutting-tool characteristics for turning operation. Only a few pieces of research have focused on correlating the vibrations and stress with wear characteristics. This research article deals with stress induced in silicon carbide tool inserts and coated tool inserts while machining SS304 steel. Since this material is much less resistant to corrosion and oxidation it is widely used in engineering applications such as cryogenics, the food industry and liquid contact surfaces. Moreover, these materials have much lower magnetic permeability so they are used as nonmagnetic engineering components which are very hard. This article focuses on the machining of SS304 by carbide tool inserts and then, the cutting forces were observed with a tool dynamometer. Using observed cutting forces, the induced stress in the lathe tool insert was determined by FEA investigation. This research also formulates an idea to predict the tool wear due to vibration. Apparently, the worn-out tool vibrates more than new tools. Using the results, the relation between stress, strain and feed rate, depth of cut and speed was found and mathematically modeled using MINI TAB. It was observed that carbide tool inserts with coating withstand better than uncoated tools while machining SS304. The results were anticipated and correlation between the machining parameters furnished the prediction of tool life and obtaining the best machining outcomes by using coated tool inserts.
Short and Ultra-Short Implants, in Association with Simultaneous Internal Sinus Lift in the Atrophic Posterior Maxilla: A Five-Year Retrospective Study
Authors: Lombardo Giorgio , Signoriello Annarita , Marincola Mauro , Liboni Pietro , Faccioni Paolo , Zangani Alessandro , D’Agostino Antonio , Nocini Pier Francesco
Published: November 2022

Recent short-term studies suggested the use of short and ultra-short implants in association with a modified osteotome sinus floor elevation (internal sinus lift) technique for the treatment of edentulous resorbed posterior maxilla. The aim of this retrospective study was to investigate this hypothesis in locking-taper implants with a mid-term follow-up of 5 years. Overall, 155 implants (32, 100, and 23 of, respectively, 5.0 mm, 6.0 mm, and 8.0 mm length) were positioned in the atrophic upper maxilla of 79 patients, and 151 implants were loaded with single crowns. Overall implant survival after 5 years was 94.84%. Implant survival for each length group was 93.75%, 94%, and 100% for 5.0, 6.0, and 8.0 mm length, respectively. Preoperative residual crestal bone height of 4.45 (1.3) mm increased to 9.25 (2.13) mm after implant placement and settled at 6.35 (1.73) mm after loading and at 5.25 (1.68) mm at follow-up. Elevation of the Schneiderian membrane was 4.8 (2.46) mm after implant placement, 3.06 (1.3) mm after loading, and 1.46 (1.06) mm at follow-up. Mean variations of peri-implant crestal bone loss and first bone-to-implant contact point were, respectively, −0.36 (1.3) mm and −0.62 (1.15) mm. It can be confirmed that internal sinus lift procedure revealed stable bone gain and negligible resorption at mid-term follow-up for atrophic upper crests with reduced height.
Experimental and Finite Element Study on Bending Performance of Glulam-Concrete Composite Beam Reinforced with Timber Board
Authors: Du Hao , Yuan Shengnan , Liu Peiyang , Hu Xiamin , Han Guohui
Published: November 2022

In this research, experimental research and finite element modelling of glulam-concrete composite (GCC) beams were undertaken to study the flexural properties of composite beams containing timber board interlayers. The experimental results demonstrated that the failure mechanism of the GCC beam was the combination of bend and tensile failure of the glulam beam. The three-dimensional non linear finite element model was confirmed by comparing the load-deflection curve and load-interface slip curve with the experimental results. Parametric analyses were completed to explore the impacts of the glulam beam height, shear connector spacing, timber board interlayer thickness and concrete slab thickness on the flexural properties of composite beams. The numerical outcomes revealed that with an increase of glulam beam height, the bending bearing capacity and flexural stiffness of the composite beams were significantly improved. The timber boards were placed on top of the glulam members and used as the formwork for concrete slab casting. In addition, the flexural properties of composite beams were improved with the increase of the timber board thickness. With the elevation of the shear connector spacing, the ultimate bearing capacity and bending stiffness of composite beams were decreased. The bending bearing capacity and flexural rigidity of the GCC beams were ameliorated with the increase of concrete slab thickness.
Tb3+ Ion Optical and Magneto-Optical Properties in the Cubic Crystals KTb3F10
Authors: Valiev Uygun V. , Karimov Denis N. , Ma Chong-Geng , Sultonov Odiljon Z. , Pelenovich Vasiliy O.
Published: November 2022

The optical and magneto-optical characteristics of KTb3F10 crystals in the transition region of 5D4 → 7F6 4f8 configurations of the Tb3+ ion at temperatures of 90 and 300 K were studied. The schemes of the optical transitions in the KTb3F10 crystals were constructed, and the energies of most of the Stark sublevels of the ground 7F6 and excited 5D4 multiplets of the Tb3+ ion split by the C4v symmetry crystal environment were determined. The presence of three- and two-doublet states in the energy spectra of the Tb3+ion multiplets 7F6 and 5D4, respectively, was established, which is in good agreement with theoretical predictions. The use of the wavefunctions of the Stark sublevels of multiplets split by a tetragonal crystal field and combining in the studied optical transition made it possible to explain some of the magnetic and magneto-optical features observed in the KTb3F10 single crystals.