bluuebunny/crossref_metadata_2025 huggingface dataset:
Erythroid Differentiation Regulator 1 Ameliorates Collagen-Induced Arthritis via Activation of Regulatory T Cells
Authors: Kim Myun Soo , Lee Sora , Park Sunyoung , Kim Kyung Eun , Park Hyun Jeong , Cho Daeho
Published: December 2020

Erythroid differentiation regulator 1 (Erdr1) has been identified as an anti-inflammatory factor in several disease models, including collagen-induced arthritis (CIA), but its exact mechanisms are still not fully understood. Here, the involvement of regulatory T (Treg) cells in Erdr1-improved CIA was investigated. In the CIA model, Erdr1 was confirmed to reduce collagen-specific IgM in plasma and plasma cells in draining lymph nodes. Importantly, the downregulated Treg cell ratio in draining lymph nodes from CIA mice was recovered by Erdr1 treatment. In addition, administration of Erdr1 improved the CIA score and joint tissue damage, while it revealed no effect in Treg cell-depleted CIA mice, indicating that Treg cells mediate the therapeutic effects of Erdr1 in the CIA model. Results from in vitro experiments also demonstrated that Erdr1 significantly induced Treg cell differentiation and the expression of Treg activation markers, including CD25, CD69, and CTLA4 in CD4+Foxp3+ cells. Furthermore, Erdr1-activated Treg cells dramatically suppressed the proliferation of responder T cells, suggesting that they are functionally active. Taken together, these results show that Erdr1 induces activation of Treg cells and ameliorates rheumatoid arthritis via Treg cells.
Identification of Novel Potential Genes Involved in Cancer by Integrated Comparative Analyses
Authors: Monticolo Francesco , Palomba Emanuela , Chiusano Maria Luisa
Published: December 2020

The main hallmarks of cancer diseases are the evasion of programmed cell death, uncontrolled cell division, and the ability to invade adjacent tissues. The explosion of omics technologies offers challenging opportunities to identify molecular agents and processes that may play relevant roles in cancer. They can support comparative investigations, in one or multiple experiments, exploiting evidence from one or multiple species. Here, we analyzed gene expression data from induction of programmed cell death and stress response in Homo sapiens and compared the results with Saccharomyces cerevisiae gene expression during the response to cell death. The aim was to identify conserved candidate genes associated with Homo sapiens cell death, favored by crosslinks based on orthology relationships between the two species. We identified differentially-expressed genes, pathways that are significantly dysregulated across treatments, and characterized genes among those involved in induced cell death. We investigated on co-expression patterns and identified novel genes that were not expected to be associated with death pathways, that have a conserved pattern of expression between the two species. Finally, we analyzed the resulting list by HumanNet and identified new genes predicted to be involved in cancer. The data integration and the comparative approach between distantly-related reference species that were here exploited pave the way to novel discoveries in cancer therapy and also contribute to detect conserved genes potentially involved in programmed cell death.
L-Dopa-Decarboxylase (DDC) Is a Positive Prognosticator for Breast Cancer Patients and Epinephrine Regulates Breast Cancer Cell (MCF7 and T47D) Growth In Vitro According to Their Different Expression of Gi- Protein- Coupled Receptors
Authors: Tremmel Eileen , Kuhn Christina , Kaltofen Till , Vilsmaier Theresa , Mayr Doris , Mahner Sven , Ditsch Nina , Jeschke Udo , Vattai Aurelia
Published: December 2020

A coherence between thyroid dysfunction and breast cancer incidence exists. Thyroid hormone metabolites bind to TAAR1 (trace amine-associated receptor 1) and through that modulate the serotonergic and dopaminergic system. Catecholamines themselves are synthesized by the L-dopa decarboxylase (DDC). The aim of our study was to analyze the influence of catecholamines on the DDC expression in primary breast cancer patients and the role of DDC concerning overall survival (OS). DDC expression was analyzed by immunohistochemistry. The effect of epinephrine on the expression of DDC and the Gi- protein was analyzed on the protein level via Western blot. A viability assay was performed to test the metabolic cell viability. The overexpression of DDC in the primary tumor was associated with longer OS (p = 0.03). Stimulation with epinephrine induced the downregulation of DDC (p = 0.038) and significantly increased viability in T47D cells (p = 0.028). In contrast, epinephrine induced an upregulation of DDC and decreased the proliferation of MCF7 cells (p = 0.028). Epinephrine led to an upregulation of Gi protein expression in MCF7 cells (p = 0.008). DDC is a positive prognostic factor for OS in breast cancer patients, and it is regulated through epinephrine differently in MCF7 and T47D. DDC may represent a novel target for the treatment of breast cancer, especially concerning its interaction with epinephrine.
The Role of Short-Chain Fatty Acids in the Interplay between a Very Low-Calorie Ketogenic Diet and the Infant Gut Microbiota and Its Therapeutic Implications for Reducing Asthma
Authors: Alsharairi Naser A.
Published: December 2020

Gut microbiota is well known as playing a critical role in inflammation and asthma development. The very low-calorie ketogenic diet (VLCKD) is suggested to affect gut microbiota; however, the effects of VLCKD during pregnancy and lactation on the infant gut microbiota are unclear. The VLCKD appears to be more effective than caloric/energy restriction diets for the treatment of several diseases, such as obesity and diabetes. However, whether adherence to VLCKD affects the infant gut microbiota and the protective effects thereof on asthma remains uncertain. The exact mechanisms underlying this process, and in particular the potential role of short chain fatty acids (SCFAs), are still to be unravelled. Thus, the aim of this review is to identify the potential role of SCFAs that underlie the effects of VLCKD during pregnancy and lactation on the infant gut microbiota, and explore whether it incurs significant implications for reducing asthma.
Therapeutic Targeting Strategies for Early- to Late-Staged Alzheimer’s Disease
Authors: Kang You Jung , Diep Yen N. , Tran Minh , Cho Hansang
Published: December 2020

Alzheimer’s disease (AD) is the most common cause of dementia, typically showing progressive neurodegeneration in aging brains. The key signatures of the AD progression are the deposition of amyloid-beta (Aβ) peptides, the formation of tau tangles, and the induction of detrimental neuroinflammation leading to neuronal loss. However, conventional pharmacotherapeutic options are merely relying on the alleviation of symptoms that are limited to mild to moderate AD patients. Moreover, some of these medicines discontinued to use due to either the insignificant effectiveness in improving the cognitive impairment or the adverse side effects worsening essential bodily functions. One of the reasons for the failure is the lack of knowledge on the underlying mechanisms that can accurately explain the major causes of the AD progression correlating to the severity of AD. Therefore, there is an urgent need for the better understanding of AD pathogenesis and the development of the disease-modifying treatments, particularly for severe and late-onset AD, which have not been covered thoroughly. Here, we review the underlying mechanisms of AD progression, which have been employed for the currently established therapeutic strategies. We believe this will further spur the discovery of a novel disease-modifying treatment for mild to severe, as well as early- to late-onset, AD.
Using Mobiles to Monitor Respiratory Diseases
Authors: Zubaydi Fatma , Sagahyroon Assim , Aloul Fadi , Mir Hasan , Mahboub Bassam
Published: December 2020

In this work, a mobile application is developed to assist patients suffering from chronic obstructive pulmonary disease (COPD) or Asthma that will reduce the dependency on hospital and clinic based tests and enable users to better manage their disease through increased self-involvement. Due to the pervasiveness of smartphones, it is proposed to make use of their built-in sensors and ever increasing computational capabilities to provide patients with a mobile-based spirometer capable of diagnosing COPD or asthma in a reliable and cost effective manner. Data collected using an experimental setup consisting of an airflow source, an anemometer, and a smartphone is used to develop a mathematical model that relates exhalation frequency to air flow rate. This model allows for the computation of two key parameters known as forced vital capacity (FVC) and forced expiratory volume in one second (FEV1) that are used in the diagnosis of respiratory diseases. The developed platform has been validated using data collected from 25 subjects with various conditions. Results show that an excellent match is achieved between the FVC and FEV1 values computed using a clinical spirometer and those returned by the model embedded in the mobile application.
Biology and Biomechanics of the Heart Valve Extracellular Matrix
Authors: Kodigepalli Karthik M. , Thatcher Kaitlyn , West Toni , Howsmon Daniel P. , Schoen Frederick J. , Sacks Michael S. , Breuer Christopher K. , Lincoln Joy
Published: December 2020

Heart valves are dynamic structures that, in the average human, open and close over 100,000 times per day, and 3 × 109 times per lifetime to maintain unidirectional blood flow. Efficient, coordinated movement of the valve structures during the cardiac cycle is mediated by the intricate and sophisticated network of extracellular matrix (ECM) components that provide the necessary biomechanical properties to meet these mechanical demands. Organized in layers that accommodate passive functional movements of the valve leaflets, heart valve ECM is synthesized during embryonic development, and remodeled and maintained by resident cells throughout life. The failure of ECM organization compromises biomechanical function, and may lead to obstruction or leaking, which if left untreated can lead to heart failure. At present, effective treatment for heart valve dysfunction is limited and frequently ends with surgical repair or replacement, which comes with insuperable complications for many high-risk patients including aged and pediatric populations. Therefore, there is a critical need to fully appreciate the pathobiology of biomechanical valve failure in order to develop better, alternative therapies. To date, the majority of studies have focused on delineating valve disease mechanisms at the cellular level, namely the interstitial and endothelial lineages. However, less focus has been on the ECM, shown previously in other systems, to be a promising mechanism-inspired therapeutic target. Here, we highlight and review the biology and biomechanical contributions of key components of the heart valve ECM. Furthermore, we discuss how human diseases, including connective tissue disorders lead to aberrations in the abundance, organization and quality of these matrix proteins, resulting in instability of the valve infrastructure and gross functional impairment.
Salivary Carbohydrate-Deficient Transferrin in Alcohol- and Nicotine-Dependent Males
Authors: Waszkiewicz Napoleon , Pawłowicz Katarzyna , Okuniewska Natalia , Kwiatkowski Mikołaj , Zalewski Daniel , Wilczyńska Karolina , Szulc Agata , Galińska-Skok Beata , Konarzewska Beata , Maciejczyk Mateusz , Zalewska Anna
Published: December 2020

Serum carbohydrate-deficient transferrin (CDT), an 80 kDa glycoprotein, is one of the most commonly employed biomarkers to detect alcohol dependence. Some salivary glycoproteins such as α-amylase, clusterin, haptoglobin, light/heavy-chain immunoglobulin, and transferrin, which alter glycosylation in alcohol-dependent persons, have been suggested to be potential alcohol markers. However, their identification is based on indirect analysis of lectin glycosidic bonds and molecular weight. We investigated the CDT content in the saliva of alcohol- and nicotine-dependent men. The CDT concentration (ng/mL, ng/mg protein) was determined by an Enzyme-Linked Immunosorbent Assay (ELISA) commercial kit in 55 men: 20 healthy social drinkers (C), 10 chronic cigarette smokers (S), 10 alcohol-dependent non-smokers (A), and 15 alcohol-dependent smokers (AS). Surprisingly, there were no differences in the concentrations of CDT between the studied groups. Salivary pH was the lowest in the AS and the highest in the A group. Therefore, salivary CDT cannot be used as an alcohol dependence marker as measured by ELISA. We suggest that direct identification of glycoproteins is necessary to search for potential salivary alcohol biomarkers. Molecules smaller than 40 kDa, which easily translocate from blood to the saliva, might be preferred as salivary alcohol markers.
SARS-CoV-2 and Viral Sepsis: Immune Dysfunction and Implications in Kidney Failure
Authors: Stasi Alessandra , Castellano Giuseppe , Ranieri Elena , Infante Barbara , Stallone Giovanni , Gesualdo Loreto , Netti Giuseppe Stefano
Published: December 2020

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causal agent of coronavirus disease 2019 (COVID-19), first emerged in Wuhan, China. The clinical manifestations of patients infected with COVID-19 include fever, cough, and dyspnea, up to acute respiratory distress syndrome (ARDS) and acute cardiac injury. Thus, a lot of severe patients had to be admitted to intensive care units (ICU). The pathogenic mechanisms of SARS-CoV-2 infection are mediated by the binding of SARS-CoV-2 spikes to the human angiotensin-converting enzyme 2 (ACE-2) receptor. The overexpression of human ACE-2 is associated with the disease severity in SARS-CoV-2 infection, demonstrating that viral entry into cells is a pivotal step. Although the lung is the organ that is most commonly affected by SARS-CoV-2 infection, acute kidney injury (AKI), heart dysfunction and abdominal pain are the most commonly reported co-morbidities of COVID-19. The occurrence of AKI in COVID-19 patients might be explained by several mechanisms that include viral cytopathic effects in renal cells and the host hyperinflammatory response. In addition, kidney dysfunction could exacerbate the inflammatory response started in the lungs and might cause further renal impairment and multi-organ failure. Mounting recent evidence supports the involvement of cardiovascular complications and endothelial dysfunction in COVID-19 syndrome, in addition to respiratory disease. To date, there is no vaccine, and no specific antiviral medicine has been shown to be effective in preventing or treating COVID-19. The removal of pro-inflammatory cytokines and the shutdown of the cytokine storm could ameliorate the clinical outcome in severe COVID-19 cases. Therefore, several interventions that inhibit viral replication and the systemic inflammatory response could modulate the severity of the renal dysfunction and increase the probability of a favorable outcome.
Dengue Virus Strikes Back: Increased Future Risk of Severe Dengue Disease in Humans as a Result of Previous Exposure to Zika Virus
Authors: Martín-Acebes Miguel A. , Saiz Juan-Carlos , Jiménez de Oya Nereida
Published: December 2020

Mosquito-borne flaviviruses include medically important pathogens that are responsible for a variety of human diseases, such as dengue, Zika congenital syndrome, and West Nile fever [...]
The Impact of Credit Constraints on International Quality and Environmental Certifications: Evidence from Survey Data
Authors: Pietrovito Filomena
Published: December 2020

Environmental and quality management practices are extremely relevant for a firm’s development and international recognition. However, dealing with the standards required and obtaining an international standards certification involves costs for employee training, procedure documents, and third-party audit fees that must be paid in advance by companies. This paper attempts to analyze the impact of difficulties in accessing external financing on the likelihood of possessing the standards and the certification, for firms based in 64 countries. A crucial aspect in uncovering such a causal effect is the potential endogeneity of financial constraints with respect to international standards certification. I address this issue by adopting a bivariate probit model and a set of firm-level instrumental variables. The empirical results showed that financially constrained firms were less likely to possess the standards required and the associated international certification, and that this impact was more relevant for small and young firms.
The Challenges and Opportunities for ERM Post-COVID-19: Agendas for Future Research
Authors: Pagach Don , Wieczorek-Kosmala Monika
Published: December 2020

In this paper, we examine the impact that COVID-19 has had on enterprise risk management (ERM). Guided by the origins and philosophy of ERM, we suggest an agenda for future research on ERM in a “post-COVID-19” reality, by addressing its integrated, strategic, and value-enhancing orientation. To guide future research endeavors in ERM, which is still an evolving discipline, we present topics that would benefit from additional research attention within both risk identification and analysis, as well as the strategic dimension of ERM.
Cocoa Production and Forest Dynamics in Ivory Coast from 1985 to 2019
Authors: Yao Sadaiou Sabas Barima , Gislain Danmo Konan , Akoua Tamia Madeleine Kouakou , Jan Bogaert
Published: December 2020

The cocoa economy of Ivory Coast started in the eastern part of the country in the 1970s and spread to the central-western and then south-western regions. For nearly a decade, it has been in the West of Ivory Coast with a population increase caused by large waves of migration. This study aims to determine different factors explaining dynamics of the cocoa economy from the East to West of Ivory Coast. The method adopted consisted of processing Landsat images from 1985–2018 and an individual survey of 278 heads of households. The results obtained showed that the development of the cocoa economy led forest cover degradation with a total loss estimated at 60.80%, 46.39%, 20.76% and 51.18% of forest area in the East, Centre-West, South-West and West, respectively. The creation of new cocoa farms in the West of Ivory Coast is governed by non-native people (51.13%) settled between 2010 and 2018. About 41% of these producers come mainly from the Centre-West (25%) and the South-West (16%). In addition, 29% of producers come from the West of Ivory Coast. Despite the abiotic characteristics being considered unfavourable, the west of Ivory Coast is in the process of becoming the country’s new zone of high cocoa production.
Prebiotic Reaction Networks in Water
Authors: Tran Quoc Phuong , Adam Zachary R. , Fahrenbach Albert C.
Published: December 2020

A prevailing strategy in origins of life studies is to explore how chemistry constrained by hypothetical prebiotic conditions could have led to molecules and system level processes proposed to be important for life’s beginnings. This strategy has yielded model prebiotic reaction networks that elucidate pathways by which relevant compounds can be generated, in some cases, autocatalytically. These prebiotic reaction networks provide a rich platform for further understanding and development of emergent “life-like” behaviours. In this review, recent advances in experimental and analytical procedures associated with classical prebiotic reaction networks, like formose and Miller-Urey, as well as more recent ones are highlighted. Instead of polymeric networks, i.e., those based on nucleic acids or peptides, the focus is on small molecules. The future of prebiotic chemistry lies in better understanding the genuine complexity that can result from reaction networks and the construction of a centralised database of reactions useful for predicting potential network evolution is emphasised.
Study of Frictional Effects of Granite Subjected to Quasi-Static Contact Loading
Authors: Shariati Hossein , Saadati Mahdi , Weddfelt Kenneth , Larsson Per-Lennart , Hild Francois
Published: December 2020

The rock fragmentation response to drilling, in particular percussive drilling, is important in order to improve the efficiency of such an operation. The resulting problem includes spherical contact between the drill bit and the material and therefore, a numerical analysis of frictional effects in quasi-static spherical indentation of Bohus granite is presented. The frictional coefficient between the indenter and the granite surface is accounted for in numerical simulations. A previously determined constitutive law is used for the purpose of numerical analyses. The latter consists of a Drucker-Prager plasticity model with variable dilation angle coupled with an anisotropic damage model. Since the tensile strength is random, Weibull statistics was considered. Using a frictionless contact model, the stress state of Bohus granite corresponding to the first material failure occurrence, observed in indentation experiments, was numerically determined. However, the frictional effects, which are of interest in this study, may lead to changes in the numerically established stress state and consequently the Weibull parameters should be recalibrated. The so-called Weibull stress decreases from 120 MPa for a frictionless contact to 75 MPa for frictional contact, and the Weibull modulus from 24 to 12. It is numerically observed that the predicted force-penetration response, using the new set of Weibull parameters, is not influenced by friction. Conversely, the predicted fracture pattern, in the case of frictional contact, is similar to the case of frictionless contact, but its size is somewhat larger. Last, a parametric study analyzing the dependence of the friction coefficient is carried out and no significant changes are detected. The novelty of the present findings concerns the fact that both an advanced damage description in combination with an advanced plasticity model, both implemented for finite element analyses, is used to analyze frictional effects at granite indentation.
Cutting Force during Surface Layer Milling of Selected Aluminium Alloys
Authors: Zawada-Michałowska Magdalena , Józwik Jerzy , Legutko Stanisław , Mika Dariusz , Pieśko Paweł , Pytka Jarosław
Published: December 2020

This paper presents the analysis of cutting force during surface layer milling of selected aluminium alloys, which are widely used in the aviation industry. The cutting force is one of the most important parameters determining the machinability of the material and also provides important information about the course of the cutting. The study analysed the influence of the technological parameters, i.e., cutting speed vc and depth of cut ap as well as the relation between cutting tool feed direction and rolling direction on the value of cutting force during milling of selected aluminium alloys, i.e., EN AW-2017A T451 and EN AW-2024 T351. The material anisotropy is a very important issue, since the engineering industry faces enormous problems related to the cutting of the tested materials that are usually supplied in the form of rolled plates. The surface layer was cut due to the fact that it accumulates the greatest residual stresses. The measurement process of cutting force was performed by using 9257B Kistler piezoelectric dynamometer. As part of the analysis of the results, the measurement uncertainty was also estimated, which was determined on the basis of two components obtained by using the A and B methods, respectively.
The Influence of Heat Treatment on Low Cycle Fatigue Properties of Selectively Laser Melted 316L Steel
Authors: Kluczyński Janusz , Śnieżek Lucjan , Grzelak Krzysztof , Torzewski Janusz , Szachogłuchowicz Ireneusz , Oziębło Artur , Perkowski Krzysztof , Wachowski Marcin , Małek Marcin
Published: December 2020

The paper is a project continuation of the examination of the additive-manufactured 316L steel obtained using different process parameters and subjected to different types of heat treatment. This work contains a significant part of the research results connected with material analysis after low-cycle fatigue testing, including fatigue calculations for plastic metals based on the Morrow equation and fractures analysis. The main aim of this research was to point out the main differences in material fracture directly after the process and analyze how heat treatment affects material behavior during low-cycle fatigue testing. The mentioned tests were run under conditions of constant total strain amplitudes equal to 0.30%, 0.35%, 0.40%, 0.45%, and 0.50%. The conducted research showed different material behaviors after heat treatment (more similar to conventionally made material) and a negative influence of precipitation heat treatment of more porous additive manufactured materials during low-cycle fatigue testing.
Enhanced Fatigue and Durability Properties of Natural Rubber Composites Reinforced with Carbon Nanotubes and Graphene Oxide
Authors: Guo Hao , Ji Peizhi , Halász István Zoltán , Pirityi Dávid Zoltán , Bárány Tamás , Xu Zongchao , Zheng Long , Zhang Liqun , Liu Li , Wen Shipeng
Published: December 2020

Fibrous carbon nanotubes (CNTs) and lamellar graphene oxide (GO) exhibit significant advantages for improving the fatigue properties of rubber composites. In this work, the synergistic effect of CNTs and GO on the modification of the microstructure and fatigue properties of natural rubber (NR) was comprehensively investigated. Results showed that CNTs and GO were interspersed, and they formed a strong filler network in the NR matrix. Compared with those of CNT/NR and GO/NR composites, the CNT-GO/NR composites showed the smallest crack precursor sizes, the lowest crack growth rates, more branching and deflections, and the longest fatigue life.
Thermomechanical Processing of a Near-α Ti Matrix Composite Reinforced by TiBw
Authors: Feng Hong , Sun Yonggang , Lian Yuzhou , Zhang Shuzhi , Zhang Changjiang , Xu Ying , Cao Peng
Published: December 2020

To further improve the mechanical properties of the as-cast 7.5 vol.% TiBw/Ti–6Al–2.5Sn–4Zr–0.7Mo–0.3Si composite, multi-directional forging (MDF) and subsequent heat treatments were carried out to adjust TiB whiskers (TiBw) and matrix characteristics. The effect of various microstructures on the tensile properties and fracture toughness of the composites was analyzed in this paper. After MDF, the TiBw are broken into short rods with a low aspect ratio and display a random distribution. Moreover, distinct microstructures were obtained after thermomechanical processing and different heat treatments. Both room-temperature and high-temperature tensile strength and ductility are improved after thermomechanical processing. By increasing the solution-treatment temperature, the microstructures transform from equiaxed to fully lamellar. A simultaneous improvement of the room-temperature and high-temperature properties is associated with the microstructural changes. In addition, the fracture toughness exhibits an increasing trend as the volume fraction of equiaxial α phases decreases. The lamellar microstructure demonstrates excellent fracture toughness due to deflection of the crack propagation path.
On the stability of radical septic functional equations
Authors: Guariglia Emanuel , Tamilvanan Kandhasamy
Published: December 2020

This paper deals with the approximate solution of the following functional equation fx7+y77=f(x)+f(y), where f is a mapping from R into a normed vector space. We show stability results of this equation in quasi-β-Banach spaces and (β,p)-Banach spaces. We also prove the nonstability of the previous functional equation in a relevant case.
Improved Isolation Procedures for Okadaic Acid Group Toxins from Shellfish (Mytilus edulis) and Microalgae (Prorocentrum lima)
Authors: Kilcoyne Jane , Burrell Stephen , Nulty Cíara , Salas Rafael , Wright Elliott J. , Rajotte Isabelle , Miles Christopher O.
Published: December 2020

Okadaic acid (OA) group toxins may accumulate in shellfish and can result in diarrhetic shellfish poisoning when consumed by humans, and are therefore regulated. Purified toxins are required for the production of certified reference materials used to accurately quantitate toxin levels in shellfish and water samples, and for other research purposes. An improved procedure was developed for the isolation of dinophysistoxin 2 (DTX2) from shellfish (M. edulis), reducing the number of purification steps from eight to five, thereby increasing recoveries to ~68%, compared to ~40% in a previously reported method, and a purity of >95%. Cell densities and toxin production were monitored in cultures of Prorocentrum lima, that produced OA, DTX1, and their esters, over ~1.5 years with maximum cell densities of ~70,000 cells mL−1 observed. Toxin accumulation progressively increased over the study period, to ~0.7 and 2.1 mg L−1 of OA and DTX1 (including their esters), respectively, providing information on appropriate harvesting times. A procedure for the purification of OA and DTX1 from the harvested biomass was developed employing four purification steps, with recoveries of ~76% and purities of >95% being achieved. Purities were confirmed by LC-HRMS, LC-UV, and NMR spectroscopy. Additional stability observations led to a better understanding of the chemistry of these toxins.
Comparison of Extraction Ability between a Mixture of Alamine 336/Aliquat 336 and D2EHPA and Ionic Liquid ALi-D2 from Weak Hydrochloric Acid Solution
Authors: Nguyen Viet Nhan Hoa , Le Minh Nhan , Lee Man Seung
Published: December 2020

The development of extraction systems to improve the extraction efficiency of metals using commercial extractants and ionic liquids is of importance. The extraction behavior of Co(II) between mixture of Alamine 336/Aliquat 336 and D2EHPA and synthesized ionic liquid ALi-D2 was compared in this work. Some factors, such as equilibrium pH, properties of the extractants, and concentration of components in the mixture had a remarkable effect on the extraction of Co(II). The interactions occurring in the mixtures as well as the change in solution pH were analyzed. Co(II) was completely extracted by ionic liquid when equilibrium pH was higher than 6.5, while it was difficult to extract Co(II) by employing the mixture of D2EHPA and Alamine 336/Aliquat 336. The formation of ionic liquid in the mixture of D2EHPA and Aliquat 336 was verified through FT-IR spectra. In addition, the competition extraction of hydrogen ion and Co(II) by ionic liquid ALi-D2 was explained. Among the three kinds of extractants, the ionic liquid showed the best extraction efficiency for Co(II) and pH control from weak acidic solutions. The present study provides valuable information on the extraction behavior of metal ion by the mixtures of commercial extractants, and thus can give some light on the development of metal extraction systems.
Effects of Ti6Al4V Surfaces Manufactured through Precision Centrifugal Casting and Modified by Calcium and Phosphorus Ion Implantation on Human Osteoblasts
Authors: Jörg Fiedler , Betül Katmer Amet , Heiner Michels , Gerhard Kappelt , Erwin Brenner Rolf
Published: December 2020

(1) In order to enable a more widespread use of uncemented titanium-based endoprostheses to replace cobalt-containing cemented endoprostheses for joint replacement, it is essential to achieve optimal osseointegrative properties and develop economic fabrication processes while retaining the highest biomedical quality of titanium materials. One approach is the usage of an optimized form of Ti6Al4V-precision casting for manufacturing. Besides the chemical and physical properties, it is necessary to investigate possible biological influences in order to test whether the new manufacturing process is equivalent to conventional methods. (2) Methods: Primary human osteoblasts were seeded on discs, which were produced by a novel Ti6Al4V centrifugal-casting process in comparison with standard machined discs of the same titanium alloy. In a second step, the surfaces were modified by calcium or phosphorus ion beam implantation. In vitro, we analyzed the effects on proliferation, differentiation, and apoptotic processes. (3) Results: SEM analysis of cells seeded on the surfaces showed no obvious differences between the reference material and the cast material with or without ion implantation. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) proliferation assay also did not reveal any significant differences. Additionally, the osteogenic differentiation process tested by quantitative polymerase chain reactions (PCR), Alizarin red S assay, and C-terminal collagen type I propeptide (CICP) Elisa was not significantly modified. No signs of induced apoptosis were observed. (4) Conclusions: In this study, we could show that the newly developed process of centrifugal casting generated a material with comparable surface features to standard machined Ti6Al4V material. In terms of biological impact on primary human osteoblasts, no significant differences were recognized. Additional Ca- or P-ion implantation did not improve or impair these characteristics in the dosages applied. These findings indicate that spin casting of Ti6Al4V may represent an interesting alternative to the production of geometrically complex orthopedic implants.
Femtosecond Laser Direct Writing of Integrated Photonic Quantum Chips for Generating Path-Encoded Bell States
Authors: Li Meng , Zhang Qian , Chen Yang , Ren Xifeng , Gong Qihuang , Li Yan
Published: December 2020

Integrated photonic quantum chip provides a promising platform to perform quantum computation, quantum simulation, quantum metrology and quantum communication. Femtosecond laser direct writing (FLDW) is a potential technique to fabricate various integrated photonic quantum chips in glass. Several quantum logic gates fabricated by FLDW have been reported, such as polarization and path encoded quantum controlled-NOT (CNOT) gates. By combining several single qubit gates and two qubit gates, the quantum circuit can realize different functions, such as generating quantum entangled states and performing quantum computation algorithms. Here we demonstrate the FLDW of integrated photonic quantum chips composed of one Hadamard gate and one CNOT gate for generating all four path-encoded Bell states. The experimental results show that the average fidelity of the reconstructed truth table reaches as high as 98.8 ± 0.3%. Our work is of great importance to be widely applied in many quantum circuits, therefore this technique would offer great potential to fabricate more complex circuits to realize more advanced functions.
Patterning Biological Gels for 3D Cell Culture inside Microfluidic Devices by Local Surface Modification through Laminar Flow Patterning
Authors: Loessberg-Zahl Joshua , Beumer Jelle , van den Berg Albert , Eijkel Jan , van der Meer Andries
Published: December 2020

Microfluidic devices are used extensively in the development of new in vitro cell culture models like organs-on-chips. A typical feature of such devices is the patterning of biological hydrogels to offer cultured cells and tissues a controlled three-dimensional microenvironment. A key challenge of hydrogel patterning is ensuring geometrical confinement of the gel, which is generally solved by inclusion of micropillars or phaseguides in the channels. Both of these methods often require costly cleanroom fabrication, which needs to be repeated even when only small changes need be made to the gel geometry, and inadvertently expose cultured cells to non-physiological and mechanically stiff structures. Here, we present a technique for facile patterning of hydrogel geometries in microfluidic chips, but without the need for any confining geometry built into the channel. Core to the technique is the use of laminar flow patterning to create a hydrophilic path through an otherwise hydrophobic microfluidic channel. When a liquid hydrogel is injected into the hydrophilic region, it is confined to this path by the surrounding hydrophobic regions. The various surface patterns that are enabled by laminar flow patterning can thereby be rendered into three-dimensional hydrogel structures. We demonstrate that the technique can be used in many different channel geometries while still giving the user control of key geometric parameters of the final hydrogel. Moreover, we show that human umbilical vein endothelial cells can be cultured for multiple days inside the devices with the patterned hydrogels and that they can be stimulated to migrate into the gel under the influence of trans-gel flows. Finally, we demonstrate that the patterned gels can withstand trans-gel flow velocities in excess of physiological interstitial flow velocities without rupturing or detaching. This novel hydrogel-patterning technique addresses fundamental challenges of existing methods for hydrogel patterning inside microfluidic chips, and can therefore be applied to improve design time and the physiological realism of microfluidic cell culture assays and organs-on-chips.
Overview of Power Electronic Switches: A Summary of the Past, State-of-the-Art and Illumination of the Future
Authors: Jiya Immanuel N. , Gouws Rupert
Published: December 2020

As the need for green and effective utilization of energy continues to grow, the advancements in the energy and power electronics industry are constantly driven by this need, as both industries are intertwined for obvious reasons. The developments in the power electronics industry has over the years hinged on the progress of the semiconductor device industry. The semiconductor device industry could be said to be on the edge of a turn into a new era, a paradigm shift from the conventional silicon devices to the wide band gap semiconductor technologies. While a lot of work is being done in research and manufacturing sectors, it is important to look back at the past, evaluate the current progress and look at the prospects of the future of this industry. This paper is unique at this time because it seeks to give a good summary of the past, the state-of-the-art, and highlight the opportunities for future improvements. A more or less ‘forgotten’ power electronic switch, the four-quadrant switch, is highlighted as an opportunity waiting to be exploited as this switch presents a potential for achieving an ideal switch. Figures of merit for comparing semiconductor materials and devices are also presented in this review.
A New MIMU/GNSS Ultra-Tightly Coupled Integration Architecture for Mitigating Abrupt Changes of Frequency Tracking Errors
Authors: Liu Shiming , Li Sihai , Fu Qiangwen , Tao Yuanbo , Wu Feng
Published: December 2020

We present a new ultra-tightly coupled (UTC) integration architecture of a micro-electromechanical inertial measurement unit (MIMU) and global navigation satellite system (GNSS) to reduce the performance degradation caused by abrupt changes of frequency tracking errors. A large frequency error will lead to a decrease in the carrier-to-noise ratio (C/N0) estimate and an increase in the code discriminator estimation error. The disruptive effects of frequency errors on the estimation of C/N0 and on the code discriminator are quantitatively evaluated via theoretical analyses and Monte Carlo simulations. The new MIMU/GNSS UTC architecture introduces a large frequency error detector and a refined frequency processor based on a retuned frequency in each tracking channel. In addition, an adaptive channel prefilter with multiple fading factors is introduced as an alternate to the conventional prefilter. Numerical simulations based on a highly dynamic trajectory are used to assess performance. The simulation results show that when there is an abrupt step change in the frequency tracking error, the new UTC architecture can effectively suppress the divergence of navigation solutions and the loss of tracking lock, and can significantly reduce the deviation of the C/N0 estimation.
Validating Thermal Lethality to Salmonella enterica in Chicken Blood by Simulated Commercial Rendering
Authors: Wong de la Rosa Caleb , Daniels Kourtney A. , Moreira Rosana G. , Kerth Chris R. , Taylor Thomas M.
Published: December 2020

The U.S. rendering industry produces materials for use in further processed animal foods and feeds and is required to scientifically validate food safety hazard control. This study aimed to provide lethality validation for Salmonella enterica during simulated commercial rendering of whole chicken blood. Chicken blood was inoculated with a blend of multiple serovars of the pathogen (S. Heidelberg, Typhimurium, Senftenberg) and subjected to heating at 82.2, 87.8, or 93.3 °C; surviving cells were enumerated incrementally up to 5.0 min. Survivor data were modeled using the GInaFiT 1.7 freeware package. D-values and t7D (time to a 7.0 log10-cycle inactivation) values were generated from best-fit model parameters. Predictive modeling analysis revealed that the survival curves of Salmonella possessed log-linear components but also possessed shoulder and/or tail components. Mean D-values declined from 0.61 to 0.12 min as heating temperature was raised from 82.2 to 93.3 °F, respectively, differing by heating temperature (p = 0.023). t7D values differed significantly by heating temperature (p = 0.001), as was also the case for shoulder length (SL) (p = <0.0001), where, at lower temperatures, a shoulder was observed versus heating at 93.3 °F. These data aid scientific validation of Salmonella enterica inactivation during thermal rendering of poultry blood for use in further processed animal foods.
Single-Atom Catalysts for Biotherapy Applications: A Systematic Review
Authors: Jiang Shan , Zhang Chengfei , Zou Ting
Published: December 2020

Single-atom catalysts (SACs), as atomically dispersed metal active sites anchored or coordinated on suitable supports, demonstrate large potential for use in therapeutic applications. SACs have structural features similar to those of natural enzyme, while exhibiting remarkable catalytic activity, desirable stability, and excellent selectivity. This systematic review aims to synthesize evidence on SACs’ biotherapy applications. Three databases (PubMed/MEDLINE, ISI Web of Science, and ScienceDirect) were searched to identify the studies that investigated the therapeutic efficacy of SACs. A total of 12 studies that fulfilled the inclusion criteria were included and reviewed, and the key findings were qualitatively synthesized. Overall, various SACs were investigated for biotherapy applications, including anticancer, anti-infection (antibacterial), and anti-inflammatory applications; brain trauma therapies, and oxidative-stress cytoprotection applications. All of the included studies showed that the synthesized SACs demonstrated superior therapeutic effects compared with their respective controls. Among the 12 studies reviewed, 11 studies showed satisfied biocompatibility of the applied SACs, whereas minimal cytotoxicity was reported in 1 study. Collectively, the reviewed studies indicated that SACs exhibited considerable promise in the field of biotherapy. Additional studies are needed for a better understanding of the effect of SACs in the treatment of various diseases.
Low-Temperature Synthesis of Monolithic Titanium Carbide/Carbon Composite Aerogel
Authors: Niu Tingting , Zhou Bin , Zhang Zehui , Ji Xiujie , Yang Jianming , Xie Yuhan , Wang Hongqiang , Du Ai
Published: December 2020

Resorcinol-formaldehyde/titanium dioxide composite (RF/TiO2) gel was prepared simultaneously by acid catalysis and then dried to aerogel with supercritical fluid CO2. The carbon/titanium dioxide aerogel was obtained by carbonization and then converted to nanoporous titanium carbide/carbon composite aerogel via 800 °C magnesiothermic catalysis. Meanwhile, the evolution of the samples in different stages was characterized by X-ray diffraction (XRD), an energy-dispersive X-ray (EDX) spectrometer, a scanning electron microscope (SEM), a transmission electron microscope (TEM) and specific surface area analysis (BET). The results showed that the final product was nanoporous TiC/C composite aerogel with a low apparent density of 339.5 mg/cm3 and a high specific surface area of 459.5 m2/g. Comparing to C aerogel, it could also be considered as one type of highly potential material with efficient photothermal conversion. The idea of converting oxide–carbon composite into titanium carbide via the confining template and low-temperature magnesiothermic catalysis may provide new sight to the synthesis of novel nanoscale carbide materials.
The Diverse Efficacy of Food-Derived Proanthocyanidins for Middle-Aged and Elderly Women
Authors: Izumi Toru , Terauchi Masakazu
Published: December 2020

Middle-aged and elderly women are affected by various symptoms and diseases induced by estrogen deficiency. Proanthocyanidins, widely present in many kinds of fruits and berries, have many beneficial effects, such as antioxidative, anti-inflammatory, and antimicrobial activities. We researched the effects of proanthocyanidins for middle-aged and elderly women, finding that it has been revealed in many clinical trials and cohort studies that proanthocyanidins contribute to the prevention of cardiovascular disease, hypertension, obesity, cancer, osteoporosis, and urinary tract infection, as well as the improvement of menopausal symptoms, renal function, and skin damage. Thus, proanthocyanidins can be considered one of the potent representatives of complementary alternative therapy.
The Association between Purine-Rich Food Intake and Hyperuricemia: A Cross-Sectional Study in Chinese Adult Residents
Authors: Aihemaitijiang Sumiya , Zhang Yaqin , Zhang Li , Yang Jiao , Ye Chen , Halimulati Mairepaiti , Zhang Wei , Zhang Zhaofeng
Published: December 2020

Objective: To explore the correlation between purine-rich food intake and hyperuricemia in Chinese adult residents. Method: A cross-sectional study was conducted on the purine-rich food intake of Chinese adult residents based on the China Health and Nutrition Survey (CHNS) in 2009. The subjects were divided into hyperuricemia group and nonhyperuricemia group according to serum uric acid level, and the differences of the sociodemographic information (age, gender, and region), health status (weight status, blood pressure, blood sugar status), living habits (alcohol consumption, smoking status) and food intake (purine-rich food, other food) were compared between the two groups. Logistic regressions investigated the associations between the daily intake of purine-rich food (animal-derived food and legumes) and hyperuricemia. Results: Eventually, 6813 subjects were included in our study, 1111 of them had hyperuricemia. The intake of seafood, legumes, red meat, and poultry all increased the risk of hyperuricemia (p < 0.05), while the intake of purine-rich fungi and purine-rich vegetables did not affect the occurrence of hyperuricemia. Animal-derived food was the main source of purine-rich food consumed by Chinese adult residents (140.67g/day), which had a great impact on hyperuricemia. Finally, after adjusting for gender, age, region, body mass index (BMI), alcohol consumption, hypertension, and refined grains intake, the risk of hyperuricemia increased by 2.40% and 1.10% for each increase of 10 g in animal-derived food intake (OR = 1.024, 95% CI: 1.018–1.030) and legumes intake (OR = 1.011, 95% CI: 1.003–1.019), respectively. Conclusion: The intake of animal-derived food and legumes were positively correlated with the occurrence of hyperuricemia. Controlling the intake of animal-derived food and legumes would be more beneficial to controlling the risk of hyperuricemia.