bluuebunny/crossref_metadata_2025 huggingface dataset:
Path-Tracking Ability of the ASV on Different Adhesion Coefficient Roads Based on Slide Mode Control
Authors: Zhong Shengzhi , Peng Dengzhi , Huang Bin , Ma Liutao
Published: December 2023

A vehicle equipped with an articulated steering system is often used in a particular area, such as a mine or the construction field. These sealing or semi-sealing fields make autonomous driving by an ASV (Articulated Steering Vehicle) easier than in passenger cars. Path-tracking is essential for an autonomous vehicle. To improve the path-tracking ability of an ASV, a path-tracking model is established based on dynamic theory. Then, the slide-mode predictive-control (SMPC) strategy is initiated to design the path-tracking controller. The improvement in the path-tracking ability on roads with different adhesion coefficients is validated via the HIL (Hardware in Loop) platform. According to the simulation in HIL with the provided strategy, the path-tracking error during the typical condition decreased by 21.73~93.84%, demonstrating the SMC controller can be used to improve the path-tracking performance of an ASV.
Technoeconomic Analysis of Torrefaction and Steam Explosion Pretreatment Prior to Pelletization of Selected Biomass
Authors: Onyenwoke Chukwuka , Tabil Lope G. , Dumonceaux Tim , Mupondwa Edmund , Cree Duncan , Li Xue , Onu Olughu Onu
Published: December 2023

Lignocellulosic biomass has demonstrated great potential as feedstock for pellet production, notwithstanding the fact that the industrial production of pellets is faced with some economic challenges. This study presents a technoeconomic analysis of six scenarios to develop a process model for pellet production from sawdust and oat straw that employs torrefaction and steam explosion pretreatment prior to pelletization. SuperPro Designer was used to carry out this evaluation. The pellet plants were designed to have a capacity of 9.09 t/h of sawdust and oat straw each. The pellet yield ranged from 59 kt to 72 kt/year. The scenarios analyzed included variations of steam explosion and torrefaction. In some scenarios, materials were lost in the form of liquid and gas due to the pretreatment process. The breakdown of equipment purchase cost showed that the torrefaction reactor is the most expensive unit with approximately 51% of the purchase cost. Facility-dependent and feedstock costs were the major significant contributors to the pellet production cost. The minimum selling prices of the pellets obtained from Scenarios 1–6 were $113.4/t, $118.7/t, $283.4/t, $298.7/t, $200.5/t, and $208.4/t, respectively. The profitability of pellet production as determined by the net present value (NPV), internal rate of return (IRR), and payback period was found to be sensitive to variations in feedstock cost.
Environmentally Safe Method for Conditioning and Dewatering Sewage Sludge Using Iron Coagulant, Cellulose and Perlite
Authors: Kamizela Tomasz , Worwąg Małgorzata , Kowalczyk Mariusz
Published: December 2023

A reasonable strategy for the development of sludge conditioning methods prior to dewatering appears to be the use of substances that allow the safe management of dewatered sludge. It is also justified to use mineral or organic conditioners instead of synthetic chemicals, e.g., polyelectrolytes, or to try to use other substances, e.g., waste. The properties of iron coagulant (PIX 113) combined with perlite and cellulose can be an environmentally safe method of sludge conditioning. The tests were carried out in accordance with European standards on the efficiency of mechanical dewatering of sewage sludge. The most advantageous method of sludge conditioning was the dosing of the iron coagulant PIX 113. The use of at least a coagulant dose of 0.40 g/g DS enabled the achievement of minimum sludge dewatering parameters, i.e., specific resistance of filtration (SRF) < 5.0 E12 m/kg and final hydration of filtration cake (FH) < 80%. The use of cellulose and perlite as stand-alone conditioners or in combination with PIX 113 resulted in a deterioration of the sludge dewaterability and the quality of the filtrate. It is assumed that the further development of environmentally friendly conditioning methods requires the use of easy-to-use, non-toxic and biodegradable substances. It is important to select conditioners which, in practically acceptable doses, can improve the conditioning effect or show a synergistic effect in combination with previously used conditioners.
Oil from Mullet Roe Byproducts: Effect of Oil Extraction Method on Human Erythrocytes and Platelets
Authors: Tsamesidis Ioannis , Tzika Paraskevi , Georgiou Despoina , Charisis Aggelos , Hans Sakshi , Lordan Ronan , Zabetakis Ioannis , Kalogianni Eleni P.
Published: December 2023

Background: The valorization of byproducts to obtain high nutritional value foods is of utmost importance for our planet where the population is booming. Among these products are oils rich in ω-3 fatty acids produced from fishery byproducts. Recently, mullet roe oil from roe byproducts was produced that was rich in the ω-3 fatty acids eicosatetraenoic acid (EPA) and docosahexaenoic acid (DHA). Oils are customarily characterized for their composition and degree of oxidation but little is known of their biological effects, especially the effect of the extraction method. Methods: The purpose of this study was to evaluate the effects of freshly extracted mullet roe oil from mullet roe byproducts and the effect of the extraction method on human red blood cells (hRBCs) and platelets. To this end, the hemocompatibility (cytotoxicity), oxidative effects, and erythrocyte membrane changes were examined after 1 and 24 h of incubation. Antiplatelet effects were also assessed in vitro. Results: The expeller press oil extraction method and alcalase-assisted extraction produced the most biocompatible oils, as shown by hemocompatibility measurements and the absence of erythrocyte membrane alterations. Solvent extracts and protease-assisted extraction oils resulted in the rupture of red blood cells at different examined dilutions, creating hemolysis. Conclusions: It seems that the proper functioning of oil–erythrocyte interactions cannot be explained solely by ROS. Further investigations combining chemical analysis with oil–cell interactions could be used as an input to design high nutritional value oils using green extraction technologies. All samples exhibited promising antiplatelet and antiblood clotting effects in vitro.
Conversion of Retinyl Palmitate to Retinol by Wheat Bran Endogenous Lipase Reduces Vitamin A Stability
Authors: Van Wayenbergh Eline , Blockx Jonas , Langenaeken Niels A. , Foubert Imogen , Courtin Christophe M.
Published: December 2023

Wheat bran can be used as a cost-effective food ingredient to stabilise vitamin A. However, wheat bran endogenous enzymes have been shown to reduce vitamin A stability. In this study, we elucidated the mechanism for this negative effect in an accelerated storage experiment with model systems consisting of native or toasted wheat bran, soy oil and retinyl palmitate (RP). Both native and toasted wheat bran substantially stabilised RP. While RP was entirely degraded after ten days of storage in the absence of wheat bran, the RP retention after ten days was 22 ± 2% and 75 ± 5% in the presence of native and toasted bran, respectively. The significantly stronger stabilising effect of toasted bran was attributed to the absence of bran endogenous enzymes. In contrast to toasted bran systems, noticeable free fatty acid production was observed for native bran systems. However, this did not result in a pronounced lipid oxidation. Next to lipid hydrolysis, wheat bran lipase was shown to hydrolyse retinyl esters to the less stable retinol and fatty acids. This reaction could explain the major part, about 66 ± 5%, of the difference in RP stabilisation between native and toasted wheat bran.
Postharvest Quality Improvement of Tomato (Solanum lycopersicum L.) Fruit Using a Nanomultilayer Coating Containing Aloe vera
Authors: Flores-López María L. , Vieira Jorge M. , Rocha Cristina M. R. , Lagarón José M. , Cerqueira Miguel A. , Jasso de Rodríguez Diana , Vicente António A.
Published: December 2023

The effectiveness of an alginate/chitosan nanomultilayer coating without (NM) and with Aloe vera liquid fraction (NM+Av) was evaluated on the postharvest quality of tomato fruit at 20 °C and 85% relative humidity (RH) to simulate direct consumption. Both nanomultilayer coatings had comparable effects on firmness and pH values. However, the NM+Av coating significantly reduced weight loss (4.5 ± 0.2%) and molds and yeasts (3.5–4.0 log CFU g−1) compared to uncoated fruit (16.2 ± 1.2% and 8.0 ± 0.0 log CFU g−1, respectively). It notably lowered O2 consumption by 70% and a 52% decrease in CO2 production, inhibiting ethylene synthesis. Visual evaluation confirmed NM+Av’s efficacy in preserving the postharvest quality of tomato. The preservation of color, indicated by the Minolta color (a*/b*) values, demonstrated NM+Av’s ability to keep the light red stage compared to uncoated fruit. The favorable effects of NM+Av coating on enhancing postharvest quality indicates it as a potential alternative for large-scale tomato fruit preservation.
Explaining Chinese Consumers’ Continuous Consumption Intention toward Prepared Dishes: The Role of Perceived Risk and Trust
Authors: Zhang Weihua , Zheng Jiaqiang , Li Yufeng
Published: December 2023

With the rapid development and the increasing importance of the consumer market of prepared dishes in China, it is imperative to study the formation mechanism of Chinese consumers’ continuous consumption intention (CCI) toward prepared dishes for promoting the sustainable development of Chinese prepared dishes industry. The aim of this study is to study the formation mechanism and the role of perceived risk and trust in it. Based on the Expectation Confirmation Model, this study constructed a model of continuous consumption intention of prepared dishes by introducing perceived risk and trust. 381 adult consumers were surveyed and the data was analyzed by an Exploratory Factor Analysis and the Partial Least Squares Structure Equation Model. The results showed that: (1) The confirmation of expectation had a significant positive impact on trust and satisfaction, a significant negative impact on the perceived risk (PR). Trust and satisfaction had a significant positive, while PR had a significant negative, impact on CCI. (2) The dimensions of PR included social, health, quality, psychological and purchasing risk, among which quality risk had a significant negative impact on CCI. (3) All dimensions of trust (ability, integrity, benevolence and government trust) had a significant positive impact on CCI, among which integrity trust played the most important role. (4) Overall, trust had a significant negative impact on PR. Benevolence trust could mitigate all dimensions of PR, integrity trust could mitigate all dimensions of PR except health risk, and ability trust, government trust could only mitigate quality risk. Therefore, the enterprises should pay high attention to the quality of their products and the establishment and maintenance of consumer trust, and the government should establish an authoritative image.
A Sol–Gel Transition and Self-Healing Hydrogel Triggered via Photodimerization of Coumarin
Authors: Ye Yong , Wang Wenkai , Liu Xin , Chen Yong , Tian Shenghui , Fu Peng
Published: December 2023

Reversible chemical covalency provides a path to materials that can degrade and recombine with appropriate stimuli and which can be used for tissue regeneration and repair. However, designing and preparing efficient and quickly self-healing materials has always been a challenge. The preparation strategies of photoresponsive gels attract a lot of attention due to their precise spatial and temporal control and their predetermined response to light stimulation. In this work, the linear copolymer PAC was synthesized via precipitation polymerization of acrylic acid and 7-(2-acrylate-ethoxylated)-4-methylcoumarin. The coumarin groups on the copolymer PAC side chains provide a reversible chemical cross-linking via photostimulation, which achieves reversible regulation of the gel network structure. The concentration of 18 wt% PAC solution produces gelation under irradiation with 365 nm. In contrast, PAC gel is restored to soluble copolymers under irradiation with 254 nm. Meanwhile, the mechanical and self-healing properties of the gel were also explored. It is demonstrated that the cracks of the gel can be repaired simply, quickly, and efficiently. Furthermore, the PAC copolymer shows an excellent adhesion property based on the reversible sol–gel transition. Thus, the PAC gel has considerable potential for applications in engineering and biomedical materials.
Complementary Therapies and Their Association with Problems in Therapeutic Adherence to Conventional Synthetic DMARDs in Rheumatoid Arthritis: A Cross-Sectional Study
Authors: Santiago-Garcia Aline Priscilla , Gamez-Nava Jorge Ivan , Avalos-Salgado Felipe Alexis , Cerpa-Cruz Sergio , Amaya-Cabrera Edna Lizeth , Gutierrez-Ureña Sergio , Nava-Valdivia Cesar Arturo , Gonzalez-Vazquez Sergio , Arevalo-Simental Diana Esperanza , Gomez-Camarena Jose Jorge , Ponce-Guarneros Juan Manuel , Rodriguez-Jimenez Norma Alejandra , Saldaña-Cruz Ana Miriam , Cardona-Muñoz Ernesto German , Gonzalez-Lopez Laura
Published: December 2023

The use of complementary therapies is highly prevalent among patients with rheumatoid arthritis (RA). Nevertheless, the use of complementary medicine could involve problems in the following of scientifically accepted treatments. To date, there is limited information regarding the association of nonconventional therapies with problems regarding compliance with the treatment. Therefore, the objective of this study was to identify whether the utilization of complementary therapies is associated with a high risk of problems regarding therapeutic adherence to conventional synthetic disease-modifying anti-rheumatic drugs (cs-DMARDs) in RA patients. A survey was performed with RA patients in an outpatient rheumatology clinic in a university hospital; the use of complementary therapies, as well as their type, was identified. To assess problems with therapeutic adherence, we used the four-item Morisky–Green scale. A comprehensive assessment of clinical and therapeutic characteristics was performed. Univariable and multivariable models were performed to identify the risk of problems with therapeutic adherence in users of complementary therapies. In total, 250 RA patients were included; 92% used complementary therapies. Of them, the most frequently used were herbal medicine (65%), homeopathy (64%), and cannabis and its derivatives (51%). In the univariable logistic regression analysis, the factors associated with problems in the therapeutic adherence to cs-DMARDs were age (p = 0.019), the presence of other comorbidities (p = 0.047), and the use of complementary therapies (p = 0.042). After controlling for potential confounders, the use of complementary therapies increased the risk of problems with therapeutic adherence to cs-DMARDs (adjusted OR = 2.84, 95% CI = 1.06–7.63, p = 0.037). We concluded that the use of complementary therapies increases the risk of problems with therapeutic adherence. Therefore, for physicians and healthcare professionals, the early identification of the use of nonconventional therapies in their RA patients is required, followed by a directed discussion with their patients about the risks and benefits to which they could be exposed to complementary therapies.
Determinants of Prenatal Childbirth Fear during the Third Trimester among Low-Risk Expectant Mothers: A Cross-Sectional Study
Authors: Ibrahim Heba A. , Alshahrani Majed S. , Elgzar Wafaa T. Ibrahim
Published: December 2023

Background: Fear of Childbirth (FOC) can significantly impact women’s physical and psychological health; therefore, healthcare providers must provide proactive care, which means they have to intervene before FOC becomes tokophobia. This study’s purpose is to evaluate the determinants of prenatal childbirth fear during the third trimester among low-risk expectant mothers. Methods: A cross-sectional comparative study was conducted at the Maternal and Children Hospital’s outpatient clinics in Najran City, Saudi Arabia, from April to July 2023. The study involved 377 nulliparous and multiparous women, using a systemic random sampling technique. The data were collected using an interview schedule composed of questions related to demographic and obstetrics characteristics, the FOC questionnaire, and a multidimensional scale of perceived social support. Significant FOC predictors were examined using a binary logistic regression model. Results: There was a statistically significant difference between nulliparous and multiparous participants concerning FOC; 80.0% of nulliparous participants had significant FOC compared to 67.8% of multiparous participants (p = 0.011). A binary logistic regression clarified that regular antenatal care and family and spousal support were significantly negatively correlated with significant FOC among multiparous and nulliparous women (p < 0.05). For multiparas, FOC was associated with pregnancy planning and previous labor-related complications. In addition, friends’ support was an important predictor of significantly lower FOC among nulliparous women (p < 0.05). Conclusions: Significant FOC was higher in nulliparous women when compared to multiparous women. Numerous obstetric variables and different types of social support play important roles in significant FOC. Special attention and support should be provided to high-risk women for proper FOC management during prenatal classes to improve their childbirth experiences.
Exploring the Operational Status and Challenges of Community-Based Mental Healthcare Centers in Taiwan: A Qualitative Analysis of Healthcare Professionals’ Insights
Authors: Yao Ching-Teng , Hong Hong
Published: December 2023

Psychological disorders have become more prevalent in the presence of modernization and societal changes. Community-based mental health is important in healthcare. Taiwan has passed the Mental Health Act, and county governments have established community-based mental healthcare centers. This study aimed to fill the research gap regarding the operational status of these centers. A qualitative study design using semi-structured interviews was used to obtain data from a purposive sample. Seventeen healthcare professionals who were front-line workers of a community-based mental healthcare center in Taiwan were interviewed individually. This study uses the organizational analysis structure as the research base. The data were analyzed using qualitative content analysis. The theme—“operational status and difficulties”—and two categories with twelve subcategories emerged. The findings demonstrate (1) unclear objectives and imprecisely defined roles, (2) incomplete services provided, an overly defined area, and ineffectiveness, (3) the central government lacking clear objectives and operational strategies, (4) the public being ignorant of mental diseases and the operation of the centers, and (5) the lack of local resources for mental and social welfare. The government should immediately form clear policies to improve community-based mental healthcare, clarify the structure and models, increase resources for the centers, and provide direct services.
Challenges of the Implementation of a Delirium Rate Scale in a Pediatric Intensive Care Unit: A Qualitative Approach
Authors: Balsalobre-Martínez Paula , Montosa-García Raquel , Marín-Yago Ana , Baeza-Mirete Manuel , Muñoz-Rubio Gloria María , Rojo-Rojo Andrés
Published: December 2023

Introduction: Delirium in the pediatric population admitted to intensive care is a worrying reality due to its potential complications and the increase in associated costs. This study aims to explore the experiences of nursing staff of a Pediatric Intensive Care Unit after 15 months of starting a program to fight against childhood delirium in their unit. Methodology: A qualitative study was conducted through semi-structured interviews with Pediatric Intensive Care Unit (PICU) Key Informants. The Standards for Reporting Qualitative Research (SRQR) and the consolidated criteria for Reporting Qualitative Research (COREQ) were followed as quality measures for the study. Seven nurses (33% of the eligible population) from the PICU of a referral hospital were interviewed. Text transcripts were analyzed using the Interpretative Description and Qualitative Content Analysis method. Results: The interviewees indicated not identifying delirium as an important reality; with great deficiencies observed in what is related to the identification of delirium; identifying CAPD as an unreliable tool in their unit; and not sharing therapeutic objectives in this respect with the medical staff. Conclusions: The nursing staff presented a series of negative attitudes towards the phenomena of delirium in their unit, with gaps in training and in clinical management, and the diagnostic tool used, and did not see it as a priority objective of the unit, partly due to a resistance to change and a latent interprofessional communication conflict. A change at the formative, attitudinal, and relational levels is urgently needed for the success of the program and the well-being of the children in the unit.
The Immediate Effects of Muscle Energy Technique in Chronic Low Back Pain Patients with Functional Leg Length Discrepancy: A Randomized and Placebo-Controlled Trial
Authors: Yoon Jung-Dae , Jung Jin-Hwa , Cho Hwi-Young , Shin Ho-Jin
Published: December 2023

This study was conducted to determine the effect of muscle energy technique (MET) on pelvic alignment, leg length, pain, and fatigue in chronic low back pain (CLBP) patients with leg length discrepancy (LLD). Forty-two CLBP patients with LLD volunteered to participate and were randomly assigned to the MET group (n = 21) and placebo group (n = 21). The intervention group performed three METs with 5 s of isometric contraction and 30 s of rest once, and the placebo group performed three times the placebo-MET, maintaining the same posture as the MET group without muscle isometric contraction. X-ray equipment, tape measure, and visual analog scale were used to evaluate pelvic alignment, leg length, pain, and fatigue before and after each intervention. In comparison pre- and postintervention, only the MET group showed significant changes in pelvic alignment, leg length, pain, and fatigue (p < 0.05). In comparison between groups, there were significant differences in all variables (pelvic alignment, leg length, pain, fatigue) (p < 0.05). The results of this study confirmed the therapeutic effect of MET for improving pelvic alignment, functional LLD, pain, and fatigue in CLBP patients with functional LLD. Future research is needed to evaluate the long-term effect on more chronic low back pain patients.
Experiences of Patient Safety Education and Factors Affecting the Willingness to Participate in Patient Safety in Undergraduate Nursing Students in South Korea
Authors: Shin Sun-Hwa , Baek On-Jeon
Published: December 2023

Patient safety (PS) education must be provided to medical personnel who deal with life and death situations. This study aimed to examine the current state of PS education and to investigate the factors affecting the willingness of undergraduate nursing students to participate in PS. This study was a cross-sectional descriptive correlational survey. In this study, an online survey targeting members of an online café in South Korea that prepares nursing students was conducted from 19–21 April 2023. In total, 296 undergraduate nursing students in their fourth grade at 132 nursing colleges across the country participated. The results showed that 94.6% (280 students) had experience in PS education and 45.6% (135 students) had witnessed a PS incident in clinical practice. Most students (90.9%) responded that PS education was necessary and that ‘clinical practice at the hospital’ was the most effective method of PS education. PS perception increased as PS knowledge increased, and it was confirmed that an increase in PS perception increased the willingness to participate in PS. Factors that increased nursing students’ willingness to participate in PS were high satisfaction with clinical practice, high PS education needs, and high PS perception scores. To increase nursing students’ willingness to participate in PS, it is important to improve their PS perception and ensure that they have a variety of PS education experiences, which requires the development of a standardized PS education program.
Patients’ Characterization, Pattern of Medication Use, and Factors Associated with Polypharmacy: A Cross-Sectional Study Focused on Eight Units of the Portuguese National Network for Long-Term Integrated Care
Authors: Candeias Catarina , Gama Jorge , Rodrigues Márcio , Falcão Amílcar , Alves Gilberto
Published: December 2023

The Portuguese National Network for Long-term Integrated Care (RNCCI) comprises several Units for Integrated Continuous Care (UCCIs) that provide medical, nursing, and rehabilitation care. This study aimed to evaluate the demographic and medical characteristics of patients admitted to the RNCCI, their patterns of medication use, and factors associated with polypharmacy. An observational, retrospective, cross-sectional, multicenter study was performed. This study population consisted of 180 patients. Polypharmacy status was divided into two groups: non-polypharmacy (taking ≤ 4 drugs) and polypharmacy (taking ≥ 5 drugs). Bivariate analysis and multivariate logistic regression analysis were used to determine the influence of predictor factors such as demographic and medical characteristics on the polypharmacy status during the UCCI stays. This study population (mean age of 78.4 ± 12.3 years, range 23–102 years, 59% female) was prescribed a median of 8 medications. Approximately 89.4% of the patients were taking ≥ 5 drugs, demonstrating that polypharmacy is highly prevalent in Portuguese RNCCI residents of the eight UCCIs studied. A subsequent analysis with multivariate logistic regression found that polypharmacy status was significantly associated with the unit of internment (facility) when compared to facility E with H and with the Charlson Comorbidity Index (CCI). The high prevalence of polypharmacy and the associated factors show that it is urgent to improve pharmacotherapy regimens through periodic monitoring and review of patients’ therapeutic lists, an area in which pharmacists play a very important role.
The Association between Sleep Quality and Depressive Symptoms among Stroke Survivors and Caregivers
Authors: Babkair Lisa A. , Huri Hanadi , Alharbi Walaa , Turkistani Yara , Alaslani Ruba , Alandijani Nisreen , Hakami Fatimah Hamad
Published: December 2023

Background: Stroke is a serious health problem that can lead to the development of depressive symptoms, sleep disturbances, and functional dependence in stroke survivors. The change in stroke survivors’ quality of life results in negative health outcomes for stroke survivors and caregivers. This study aims to investigate the association between sleep quality and depressive symptoms among stroke survivors and caregivers in Saudi Arabia. Methods: A cross-sectional design was used to collect data from 100 stroke survivors and 80 caregivers using the patient health questionnaire and Pittsburgh Sleep Quality Index. Results: 43% of the stroke survivors reported depressive symptoms and 65% had poor sleep quality; 21% of the caregivers reported depressive symptoms and 46% reported poor sleep quality. A strong association between sleep quality and depressive symptoms was detected for the stroke survivors and caregivers: (r = 0.885, p < 0.001); (r = 0.669, p < 0.001), respectively. A strong association was found between caregivers’ depressive symptoms and patient post-stroke depressive symptoms (r = 0.502, p < 0.001). A moderate association was found between stroke survivors’ sleep quality and caregivers’ sleep quality (r = 0.407, p < 0.001). There was a moderate association between stroke survivors’ depressive symptoms and caregivers’ sleep quality (r = 0.456, p < 0.001). Moreover, there was a moderate association between stroke survivors’ sleep quality and caregivers’ depressive symptoms (r = 0.492, p < 0.001). Conclusion: Poor sleep quality and depressive symptoms are common among stroke survivors and caregivers. This study found an association between the two variables. Therefore, depressive symptoms and sleep disturbances in stroke survivors and caregivers should receive more attention. Future research should focus on an interventional study to enhance sleep quality and decrease the risk of depressive symptoms.
Investigating Uncertainty of Future Predictions of Temperature and Precipitation in The Kerman Plain under Climate Change Impacts
Authors: Goodarzi Mohammad Reza , Heydaripour Mahnaz , Jamali Vahid , Sabaghzadeh Maryam , Niazkar Majid
Published: December 2023

Climate change affects hydroclimatic variables, and assessing the uncertainty in future predictions is crucial. This study aims to explore variations in temperature and precipitation in the Kerman Plain under climate change impacts between 2023 and 2054. For this purpose, two climate models, MRI-ESM-2 and BCC-CSM2-MR, were used to simulate precipitation and temperature under two different scenarios. The Mann–Kendall test was employed to analyze the annual time series in the future period. The results indicated an increase in the average temperature of about 1.5 degrees Celsius based on both scenarios in the coming years. Furthermore, an average annual increase of 6.37 mm of precipitation was predicted under the SSP585 scenario. Meanwhile, under the SSP585 scenario, an increase was estimated using the MRI-ESM-2 model, and a decrease was predicted with the BCC-CSM2-MR model. The Mann–Kendall test revealed a downward trend in the BCC-CSM2-MR model under both scenarios and an upward trend in the MRI-ESM-2 model under both scenarios. The bootstrap method and the R-factor index were exploited in this study with a 95% confidence interval to estimate the uncertainty of the predicted data. The results demonstrated that the predicted precipitation is more uncertain than the temperature. Finally, it is postulated that the obtained results provide necessary information for water resource management under a changing climate in the study area.
Urea/Creatinine Ratio’s Correlation with Creatine Kinase Normalization in Pediatric COVID-19 Patients with Myositis: Evaluating Prognostic and Predictive Value
Authors: Pizzo Francesco , Marino Andrea , Di Nora Alessandra , Spampinato Serena , Cacciaguerra Giovanni , Costanza Giuseppe , Scarlata Federica , Biasco Arturo , Consentino Maria Chiara , Lubrano Riccardo , Cacopardo Bruno , Nunnari Giuseppe , Ruggieri Martino , Pavone Piero
Published: December 2023

Coronavirus disease 2019 (COVID-19) has been chiefly linked with substantial respiratory complications. However, emerging studies have brought attention to the occurrence of severe muscle inflammation (myositis) related to COVID-19, potentially leading to multi-organ failure and increased mortality. Myositis is generally characterized by heightened serum creatine kinase (CK) levels. Acute myositis is characterized by an infiltration of viruses into calf muscle fibers, which may cause a subsequent inflammatory response leading to calf muscle pain. Symptomatic and supportive management, along with explanation and reassurance, is all that is required in managing this condition. While the association between myositis and severe outcomes has been recognized in adults, it remains less understood in the pediatric population. The current retrospective study, conducted at Policlinico San Marco University Hospital in Catania, aimed to analyze clinical and laboratory factors associated with myositis in pediatric patients with SARS-CoV-2 infection. Between January 2022 and January 2023, ten pediatric patients diagnosed with myositis and SARS-CoV-2 infection were evaluated. The study highlighted clinical manifestations such as fever, calf muscle pain, and abnormal gait. Lab results showed elevated CK levels among other findings. All patients underwent treatment, with the majority recovering without complications. A notable correlation was observed between CK levels, blood urea nitrogen (BUN), and the urea/creatinine ratio (UCR). The study also discusses potential pathophysiological mechanisms behind SARS-CoV-2’s impact on skeletal muscles, emphasizing an indirect inflammatory response. Our findings underscore that while myositis in children with SARS-CoV-2 infection appears to follow a benign and self-limiting trajectory, it is crucial to monitor specific markers for early intervention and management. Further research is warranted to elucidate the underlying mechanisms and improve clinical outcomes.
Explaining Theft Using Offenders’ Activity Space Inferred from Residents’ Mobile Phone Data
Authors: Liu Lin , Li Chenchen , Xiao Luzi , Song Guangwen
Published: December 2023

Both an offender’s home area and their daily activity area can impact the spatial distribution of crime. However, existing studies are generally limited to the influence of the offender’s home area and its immediate surrounding areas, while ignoring other activity spaces. Recent studies have reported that the routine activities of an offender are similar to those of the residents living in the same vicinity. Based on this finding, our study proposed a flow-based method to measure how offenders are distributed in space according to the spatial mobility of the residents. The study area consists of 2643 communities in ZG City in southeast China; resident flows between every two communities were calculated based on mobile phone data. Offenders’ activity locations were inferred from the mobility flows of residents living in the same community. The estimated count of offenders in each community included both the offenders living there and offenders visiting there. Negative binomial regression models were constructed to test the explanatory power of this estimated offender count. Results showed that the flow-based offender count outperformed the home-based offender count. It also outperformed a spatial-lagged count that considers offenders from the immediate neighboring communities. This approach improved the estimation of the spatial distribution of offenders, which is helpful for crime analysis and police practice.
Establishment of a Triple Quadrupole HPLC-MS Quantitation Method for Dystrophin Protein in Mouse and Human Skeletal Muscle
Authors: Tominari Tsukasa , Takatoya Masaru , Matsubara Toshiya , Matsunobe Michio , Arai Daichi , Matsumoto Chiho , Hirata Michiko , Yoshinouchi Shosei , Miyaura Chisato , Itoh Yoshifumi , Komaki Hirofumi , Takeda Shin’ichi , Aoki Yoshitsugu , Inada Masaki
Published: December 2023

Duchenne muscular dystrophy (DMD) is the most common type of neuromuscular disease caused by mutations in the DMD gene encoding dystrophin protein. To quantitively assess human dystrophin protein in muscle biopsy samples, it is imperative to consistently detect as low as 0.003% of the dystrophin protein relative to the total muscle protein content. The quantitation of dystrophin protein has traditionally been conducted using semiquantitative immunoblotting or immunohistochemistry; however, there is a growing need to establish a more precise quantitative method by employing liquid chromatography-mass spectrometry (LC-MS) to measure dystrophin protein. In this study, a novel quantification method was established using a mouse experiment platform applied to the clinical quantification of human dystrophin protein. The method using a spike-in approach with a triple quadrupole LC-MS quantitated the amount of dystrophin in wild-type and human DMD transgenic mice but not in DMD-null mice. In conclusion, we established a quantitating method of dystrophin using HPLC-LC-MS with a novel spike-in approach. These results indicate that our methodology could be applied to several LC-MS devices to enable the accurate measurement of dystrophin protein in patients with DMD.
BmRRS1 Protein Inhibits the Proliferation of Baculovirus Autographa californica Nucleopolyhedrovirus in Silkworm, Bombyx mori
Authors: Zhou Liqin , Ding Xinyi , Wang Zhisheng , Zhou Si , Qin Sheng , Sun Xia , Wang Xueyang , Li Muwang
Published: December 2023

The study of functional genes involved in baculovirus infection is vital for its wide application in pest biocontrol. This study utilized the Autographa californica nucleopolyhedrovirus (AcMNPV) and silkworm as models to elucidate the role of BmRRS1, which has been found to exhibit notable differential expression between resistant and susceptible silkworm strains. The results showed that it was evolutionarily conserved in selected species. Among different tissues, it was expressed at the highest level in the gonads, followed by the hemolymph and silk glands; among the different developmental stages, it was the highest in the second instar, followed by the pupae and adults. Moreover, its vital role in suppressing AcMNPV infection was verified by the decreased expression of lef3 and vp39 protein after overexpression of BmRRS1 as well as by the increased expression of the viral gene lef3 and the viral protein vp39 after siRNA treatment against BmRRS1 expression in BmN cells. Additionally, the direct interaction between BmRRS1 and AcMNPV was detected by the GST pull-down assay. Finally, the homologue of BmRRS1 in Spodoptera frugiperda was found to be involved in larval resistance to AcMNPV. In a word, BmRRS1 plays a vital role in AcMNPV resistance in silkworms, and this might be related to the direct interaction with AcMNPV. The results of this study provide a potential target for protecting silkworm larvae from virus infection and controlling agricultural and forestry pests.
Immunostimulatory Effect of Flagellin on MDR-Klebsiella-Infected Human Airway Epithelial Cells
Authors: van Linge Christine C. A. , Hulme Katina D. , Peters-Sengers Hessel , Sirard Jean-Claude , Goessens Wil H. F. , de Jong Menno D. , Russell Colin A. , de Vos Alex F. , van der Poll Tom
Published: December 2023

Pneumonia caused by multi-drug-resistant Klebsiella pneumoniae (MDR-Kpneu) poses a major public health threat, especially to immunocompromised or hospitalized patients. This study aimed to determine the immunostimulatory effect of the Toll-like receptor 5 ligand flagellin on primary human lung epithelial cells during infection with MDR-Kpneu. Human bronchial epithelial (HBE) cells, grown on an air–liquid interface, were inoculated with MDR-Kpneu on the apical side and treated during ongoing infection with antibiotics (meropenem) and/or flagellin on the basolateral and apical side, respectively; the antimicrobial and inflammatory effects of flagellin were determined in the presence or absence of meropenem. In the absence of meropenem, flagellin treatment of MDR-Kpneu-infected HBE cells increased the expression of antibacterial defense genes and the secretion of chemokines; moreover, supernatants of flagellin-exposed HBE cells activated blood neutrophils and monocytes. However, in the presence of meropenem, flagellin did not augment these responses compared to meropenem alone. Flagellin did not impact the outgrowth of MDR-Kpneu. Flagellin enhances antimicrobial gene expression and chemokine release by the MDR-Kpneu-infected primary human bronchial epithelium, which is associated with the release of mediators that activate neutrophils and monocytes. Topical flagellin therapy may have potential to boost immune responses in the lung during pneumonia.
Intracellular Trafficking of Size-Tuned Nanoparticles for Drug Delivery
Authors: Gimondi Sara , Ferreira Helena , Reis Rui L. , Neves Nuno M.
Published: December 2023

Polymeric nanoparticles (NPs) are widely used as drug delivery systems in nanomedicine. Despite their widespread application, a comprehensive understanding of their intracellular trafficking remains elusive. In the present study, we focused on exploring the impact of a 20 nm difference in size on NP performance, including drug delivery capabilities and intracellular trafficking. For that, poly(ethylene glycol) methyl ether-block-poly(lactide-co-glycolide) (PLGA-PEG) NPs with sizes of 50 and 70 nm were precisely tailored. To assess their prowess in encapsulating and releasing therapeutic agents, we have employed doxorubicin (Dox), a well-established anticancer drug widely utilized in clinical settings, as a model drug. Then, the beneficial effect of the developed nanoformulations was evaluated in breast cancer cells. Finally, we performed a semiquantitative analysis of both NPs’ uptake and intracellular localization by immunostaining lysosomes, early endosomes, and recycling endosomes. The results show that the smaller NPs (50 nm) were able to reduce the metabolic activity of cancer cells more efficiently than NPs of 70 nm, in a time and concentration-dependent manner. These findings are corroborated by intracellular trafficking studies that reveal an earlier and higher uptake of NPs, with 50 nm compared to the 70 nm ones, by the breast cancer cells. Consequently, this study demonstrates that NP size, even in small increments, has an important impact on their therapeutic effect.
Diagnosis of Familial Hypercholesterolemia in Children and Young Adults
Authors: Timoshchenko Olga , Ivanoshchuk Dinara , Semaev Sergey , Orlov Pavel , Zorina Valentina , Shakhtshneider Elena
Published: December 2023

The early detection and treatment of familial hypercholesterolemia (FH) in childhood and adolescence are critical for increasing life expectancy. The purpose of our study was to investigate blood lipid parameters, features of physical signs of cholesterol accumulation, and a personal and family history of premature cardiovascular diseases in children and young adults when FH is diagnosed. The analysis included patients under 18 years of age (n = 17) and young adults (18–44 years of age; n = 43) who received a diagnosis of FH according to clinical criteria. Targeted high-throughput sequencing was performed using a custom panel of 43 genes. A family history of cardiovascular diseases was more often noted in the group under 18 years of age than in young adults (p < 0.001). Among young adults, there was a high prevalence of typical signs of the disease such as tendon xanthomas and the early development of arterial atherosclerosis (p < 0.001). By molecular genetic testing, “pathogenic” and “probably pathogenic” variants were identified in the genes of 73.3% of patients under 18 years of age and 51.4% of patients 18–44 years of age. Thus, blood lipid screening tests combined with an accurate assessment of the family history is a highly relevant and inexpensive option for diagnosing FH in childhood. Molecular genetic testing allows us to make an accurate diagnosis and to improve adherence to treatment.
Diagnostic Algorithm to Subclassify Atypical Spitzoid Tumors in Low and High Risk According to Their Methylation Status
Authors: González-Muñoz Jose Francisco , Sánchez-Sendra Beatriz , Monteagudo Carlos
Published: December 2023

Current diagnostic algorithms are insufficient for the optimal clinical and therapeutic management of cutaneous spitzoid tumors, particularly atypical spitzoid tumors (AST). Therefore, it is crucial to identify new markers that allow for reliable and reproducible diagnostic assessment and can also be used as a predictive tool to anticipate the individual malignant potential of each patient, leading to tailored individual therapy. Using Reduced Representation Bisulfite Sequencing (RRBS), we studied genome–wide methylation profiles of a series of Spitz nevi (SN), spitzoid melanoma (SM), and AST. We established a diagnostic algorithm based on the methylation status of seven cg sites located in TETK4P2 (Tektin 4 Pseudogene 2), MYO1D (Myosin ID), and PMF1-BGLAP (PMF1-BGLAP Readthrough), which allows the distinction between SN and SM but is also capable of subclassifying AST according to their similarity to the methylation levels of Spitz nevi or spitzoid melanoma. Thus, our epigenetic algorithm can predict the risk level of AST and predict its potential clinical outcomes.
Targeting TRP Channels for Pain, Itch and Neurogenic Inflammation
Authors: Koivisto Ari-Pekka , Szallasi Arpad
Published: December 2023

Transient receptor potential (TRP) channels are multifunctional signaling molecules with important roles in health and disease [...]
Uterine Aging and Reproduction: Dealing with a Puzzle Biologic Topic
Authors: Tinelli Andrea , Andjić Mladen , Morciano Andrea , Pecorella Giovanni , Malvasi Antonio , D’Amato Antonio , Sparić Radmila
Published: December 2023

Uterine aging is the process of the senescence of uterine tissue, observed in all middle-aged mammals. Since the aging-related changes in the uterus are associated with infertility and poor pregnancy outcomes, with a lack of studies discussing uterine aging, authors reviewed uterine aging and its consequences on reproduction. MEDLINE, Scopus, and PubMed searches during the years 1990–2023 were performed using a combination of keywords and terms on such topics. According to the author’s evaluation, articles were identified, selected, and included in this narrative review. The aging process has an unfavorable impact on the uterus of mammals. There are different and selected molecular pathways related to uterine aging in humans and animals. Uterine aging impairs the function of the uterine myometrium, neurofibers of the human uterus, and human endometrium. These biological pathways modulate oxidative stress, anti-inflammatory response, inflammation, mitochondrial function, DNA damage repair, etc. All these dysregulations have a role in poorer reproductive performance and pregnancy outcomes in older mammals. The most recent data suggest that uterine aging is accompanied by genetic, epigenetic, metabolic, and immunological changes. Uterine aging has a negative impact on the reproductive performance in mammalian species, but it could be potentially modulated by pharmacological agents, such as quercetin and dasatinib.
Enhancing Immunotherapy in Ovarian Cancer: The Emerging Role of Metformin and Statins
Authors: Almeida-Nunes Diana Luísa , Silvestre Ricardo , Dinis-Oliveira Ricardo Jorge , Ricardo Sara
Published: December 2023

Ovarian cancer metastization is accompanied by the development of malignant ascites, which are associated with poor prognosis. The acellular fraction of this ascitic fluid contains tumor-promoting soluble factors, bioactive lipids, cytokines, and extracellular vesicles, all of which communicate with the tumor cells within this peritoneal fluid. Metabolomic profiling of ovarian cancer ascites has revealed significant differences in the pathways of fatty acids, cholesterol, glucose, and insulin. The proteins involved in these pathways promote tumor growth, resistance to chemotherapy, and immune evasion. Unveiling the key role of this liquid tumor microenvironment is crucial for discovering more efficient treatment options. This review focuses on the cholesterol and insulin pathways in ovarian cancer, identifying statins and metformin as viable treatment options when combined with standard chemotherapy. These findings are supported by clinical trials showing improved overall survival with these combinations. Additionally, statins and metformin are associated with the reversal of T-cell exhaustion, positioning these drugs as potential combinatory strategies to improve immunotherapy outcomes in ovarian cancer patients.