bluuebunny/crossref_metadata_2025 huggingface dataset:
Preliminary Results from an Instrumented Iceberg in the Antarctic
Authors: Kristensen Monica , Orheim Olav
Published: September 2017

A data collection platform was placed near the centre of a 950 m x 800 m near-rectangular iceberg in the Weddell Sea on 4 February 1979. Meteorological conditions and the dynamic behaviour of the iceberg were recorded for every three hours over a one-year period. The following parameters were measured: barometric pressure, wind speed and direction, air and snow temperatures, and iceberg heading, tilt, and strain. Platform elevation was 35 m a.s.l. corresponding to an iceberg thickness of about 210 m. The platform was deployed at lat. 70°5'S., long. 20°3'W, Figure 1 shows the iceberg drift and sea-ice conditions between 4 February and 4 May 1979.

Fig. 1.

Drift track of the iceberg for the period 4 February to 4 May 1979. The berg was surrounded by pack ice from about 6 March.

The tilt sensor was a Singer-Kearfott bubble-type two-axis electrolytic vertical sensing element mounted on a levelling platform. This was fixed on a 2.2 m long, 0.16 m diameter white-painted tube, which was tightly placed in a vertical drill hole in the firn, and frozen in by the addition of water (firn temperature was -5°C). The tilt sensor was level with the snow surface, and aligned so that tilt was measured along and across the main axis of the iceberg. The range of the tilt sensor was ±4.4 x 10-3 rad with a resolution of 3.7 x 10-5 rad (Bø and others 1979). The strain-meter consisted of a 1 m invar rod fixed at the passive end of the instrument to a mounting block and plate, and at the active end to the core of a linear variable differential transformer (LVDT), The LVDT body was mounted in a cage connected to a rezeroing system. The strainmeter was placed in a 1.1 m deep pit and fixed to vertical wooden posts placed 2 m deeper and frozen in. The range of the strainmeter was 1.2 x 10-3 and the resolution 10-7 (Bø and others 1979).

Tilt and strain were sampled 20 times at intervals of 6 sec. This measuring sequence was repeated every three hours. The data were stored and transmitted over the TIROS-N/NOAA satellite, which provided approximately 13 passes per day for transmission of data. Eight of these gave the location of the iceberg. Analysis has been carried out of the tilt and strain data from the instrumented iceberg for the period 4 Fébruary to 4 May 1979.
A Multi-Sensor Approach to the Interpretation of Radar Altimeter Wave Forms from Two Arctic Ice Caps
Authors: Drinkwater Mark R. , Dowdeswell Julian A.
Published: September 2017

Data collected over Svalbard on 28 June 1984 by a 13.81 GHz airborne radar altimeter enabled analysis of signals returned from two relatively large ice masses. Wave forms received over the ice caps of Austfonna and Vestfonna are analysed with the aid of existing aerial photography, radio echo-sounding data, and Landsat MSS images acquired close to the date of the altimeter flight. Results indicate that altimeter wave forms are controlled mainly by surface roughness and scattering characteristics. Wet snow surfaces have narrow 3 dB back-scatter half-angles and cause high-amplitude signals, in contrast to relatively dry snow surfaces with lower-amplitude diffuse signals. Metre-scale surface roughness primarily affects wave-form amplitude and leading-edge slope, this becoming apparent over ice streams on Vestfonna.
Antihypertensive effect of piperitenone oxide on spontaneously hypertensive rat by regulating calcium balance and reducing endothelin-1 secretion
Authors: Lan Wei , Zhang Hong Ping , Wang Ying , Jiang Min , Li Qian , An Dongqing
Published: September 2017

The purpose of this study was to investigate the antihypertensive mecha-nisms of piperitenone oxide in spontaneously hypertensive rat (SHR). Compared to control SHR, piperitenone oxide reduced the systolic blood pressure and serum endothelium-1 (ET-1). The nitric oxide (NO) production and ET-1 secretion were significantly inhibited in the noradrenaline-exposed bovine aortic endothelial cells. Piperitenone oxide showed endothelium-independent but concentration-dependent relaxation of the artery rings. The responsible mechanisms appear to be related to the modulation of ET-1 and NO levels. The vasorelaxant effect of piperitenone oxide is independent of endothelium-derived mediators, which is associated with interfering intracellular calcium homeostasis by blocking Ca2+ influx, as well as intracellular Ca2+ release.
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EFFECT OF DIETARY SUPPLEMENTATION OF GARLIC ON FEED CONVERSION RATIO, CARCASS PHYSIOGNOMIES AND HAEMATOLOGICAL PARAMETERS IN BROILERS
Authors: Islam M. F. , Haque M. N. , Parvin A. , Islam M. N. , Alam M. N. , Sikder M. H.
Published: September 2017

One of the widespread spices is garlic which consumed by people of different parts of the world but its valuable growth promoting effect is not sufficiently studied in poultry. Growth promoting effect of garlic in broiler chickens was studied together with its carcass characteristics and blood parameters. Broilers were grouped into four: A (1%), B (2%), C (positive control) and D (negative control).  Aqueous extract of garlic (1% and 2%) was prepared by extraction and were administered with drinking water. A group (1%) shows best performance with regards to body weight gain rather than B (2%), positive and negative control. At the end of experiment (35th day), in A group, the live body weight, dressed weight, feed conversion ratio, liver, gizzard, heart, spleen, and pancreas were 1833.2±2.1g (P<0.05), 1229.7±1.9g (P<0.05), 1.63(P<0.05), 47.13±0.4g, 26.15±0.7g, 9.71±1.8g, 2.21±0.2g, 2.27±0.1g respectively. The mean Total Erythrocyte Count (TEC), Haemoglobin concentration (Hb) and Packed Cell volume (PCV) were 2.9± 0.1million/cm3,7.5g ± 0.2g, and 25.8 ± 0.6%, respectively. It is recommended that 1% aqueous extraction of garlic shows better growth and improved carcass qualities in broilers.
IMPACT OF DIETARY RESTRICTION ON FOLLICULAR WAVES IN CATTLE WITH PARTICULAR REFERENCE TO ANOESTRUS
Authors: Howlader M. M. R. , Hasan M. M. I. , Atikuzzaman M. , Paul S. , Begum S.
Published: September 2017

Reproductive failure (anoestrus) is a one of the major problem in cattle production of Bangladeshdue to under feeding and unavailability of balanced ration. This study was conducted for a period from July 2009 to June 2010 to determine the nutritional impact on the follicular growth using blood hormone assays and the rate of anoestrus in cows following dietary restriction and to develop a feasible feeding technique concerning the available feed ingredients in local market. A uniform group of 2-3 years old 15 heifers were selected and were grouped randomly into three named T1(60% feeding restriction), T2(40% feeding restriction) and T3 (standard feeding). Body condition score (BCS) and body weight were measured by standard method. Hormonal assays were analyzed to determine the impacts of restricted feeding on the ovarian follicular cycle using blood sera. The average BCS and body weight was decreased significantly (p<0.01) in the animals of T1 and T2 compared to control T0. The average values of estrogen and progestrogen was decreased significantly (p<0.01) in restricted feeding heifers (T1& T2) than that of standard feeding heifers (T3). About 50% heifers showed irregular estrus and 50% showed anoestrus signs in the animals of T1 group. In addition, a total of 60% animals showed irregular estrus and 40% showed anoestrus condition in animals of T2 group.. On the other hand, the animals of control group showed regular estrous cycle with good hormonal assays. Standard feeding could improve the cattle production efficiency by overcoming the reproductive failure using available feed ingredients.
EPIDEMIOLOGICAL INVESTIGATION OF CLINICAL DISEASES AND CONDITIONS OF PET ANIMALS AT CHITTAGONG CITY AREA, BANGLADESH
Authors: Yadav U. , Zuhra F. T. , Rahman M. A. , Ahmed M. S.
Published: September 2017

This study was conducted to estimate the proportional prevalence of clinical diseases and conditions in pet animals (dog, cat, and rabbit) attended at S. A. Quaderi Teaching Veterinary Hospital, Chittagong Veterinary and Animal Sciences University (CVASU), Chittagong, Bangladesh. A total of 388 cases of different clinical diseases and conditions were recorded during the study period from October 2016 toJanuary 2017. Among them the overall proportional prevalence of clinical diseases and conditions in dog, cat and rabbit were 44.32%, 34.28% and 21.39% respectively. The medicinal case comprises the highest percentage of diseases as 79.07% and 66.92% in dog and cat, respectively. Among the medicinal cases parasitic diseases were most frequently encountered both in dog (24.42%) and cat (23.31%).  Whereas, prevalence of surgical cases were 16.28% in dog and 31.58% in cat and gynecological cases were 4.65% in dog and 1.50% in cat. In rabbit, prevalence of traumatic injuries and fractures were 16.87% followed by mange 12.06%. The parasitic cases were highest in all of the clinical diseases and conditions. So, proper prevention and therapeutic management should be taken to reduce the prevalence of parasitic diseases of pet animals. Immunoprophylaxis or vaccination and hygienic management should be performed to curb the infectious diseases. Further extensive epidemiologic, laboratory or molecular studies are also needed for the appropriate prevention and control measures of diseases and conditions of pet animals in study area.
Morphological Characterization and Determination of Aflatoxin-Production Potentials of Aspergillus flavus Isolated from Maize and Soil in Kenya
Authors: Thathana Matome , Murage Hunja , Abia Akebe , Pillay Michael
Published: September 2017

This study aimed at morphologically identifying Aspergillus flavus in soil and maize and at determining their aflatoxin-producing potentials. Five hundred and fourteen isolates obtained from maize and soil in Kenya were cultivated on Czapeck Dox Agar, Malt Extract Agar, Sabouraud Dextrose Agar, Potato Dextrose Agar, and Rose-Bengal Chloramphenicol Agar. Isolates were identified using macro-morphological characteristics. Micromorphological characteristics were determined using slide cultures. Aflatoxin production was determined by direct visual determination of the UV fluorescence of colonies on Coconut Agar Medium, Yeast Extract Sucrose agar, and Yeast Extract Cyclodextrin Sodium Deoxycholate agar and by Thin Layer Chromatography. Forty-three presumptive A. flavus isolates were identified; aflatoxin was detected in 23% of the isolates by UV fluorescence screening and in 30% by Thin-Layer Chromatography (TLC). The aflatoxins produced were: aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), and aflatoxin G1 (AFG1); some isolates produced only AFB1, whereas others produced either AFB1 and AFB2 or AFB1 and AFG1. The highest incidence of A. flavus (63%) and aflatoxin production (28%) was recorded in samples from Makueni District. Isolates from Uasin Gishu (21%) and Nyeri (5%) were non-aflatoxigenic. Bungoma District recorded 11% positive isolates of which 2% were aflatoxin producers. The occurrence of aflatoxin-producing A. flavus emphasises the need for measures to eliminate their presence in food crops.
Geographic and Research Center Origins of Rice Resistance to Asian Planthoppers and Leafhoppers: Implications for Rice Breeding and Gene Deployment
Authors: Horgan Finbarr , Srinivasan Thanga , Bentur Jagadish , Kumar Ram , Bhanu K. , Sarao Preetinder , Chien Ho , Almazan Maria , Bernal Carmencita , Ramal Angelee , Ferrater Jedeliza , Huang Shou-Horng
Published: September 2017

This study examines aspects of virulence to resistant rice varieties among planthoppers and leafhoppers. Using a series of resistant varieties, brown planthopper, Nilaparvata lugens, virulence was assessed in seedlings and early-tillering plants at seven research centers in South and East Asia. Virulence of the whitebacked planthopper, Sogatella furcifera, in Taiwan and the Philippines was also assessed. Phylogenetic analysis of the varieties using single-nucleotide polymorphisms (SNPs) indicated a clade of highly resistant varieties from South Asia with two further South Asian clades of moderate resistance. Greenhouse bioassays indicated that planthoppers can develop virulence against multiple resistance genes including genes introgressed from wild rice species. Nilaparvata lugens populations from Punjab (India) and the Mekong Delta (Vietnam) were highly virulent to a range of key resistance donors irrespective of variety origin. Sogatella furcifera populations were less virulent to donors than N. lugens; however, several genes for resistance to S. furcifera are now ineffective in East Asia. A clade of International Rice Research Institute (IRRI)-bred varieties and breeding lines, without identified leafhopper-resistance genes, were highly resistant to the green leafhopper, Nephotettix virescens. Routine phenotyping during breeding programs likely maintains high levels of quantitative resistance to leafhoppers. We discuss these results in the light of breeding and deploying resistant rice in Asia.
Bar-Wave Calibration of Acoustic Emission Sensors
Authors: Ono Kanji , Hayashi Takahiro , Cho Hideo
Published: September 2017

This study extended a bar-wave calibration method for acoustic emission (AE) sensors. It combined laser interferometer displacement measurements and the wave propagation medium of a long bar, excited at its end with an ultrasonic transducer driven by a pulser. Receiving bar-wave sensitivities of 16 types of AE sensors were measured and compared to their receiving sensitivities to normally incident waves. The two types of the receiving sensitivity always differed for a given AE sensor. The bar-wave sensitivities of R6a sensors resembled their surface-wave sensitivities, indicating that the bar-wave sensitivities can represent the surface-wave sensitivities in typical AE applications. Some bar-wave modes were identified by comparing peaks found on observed Choi-Williams transform spectrograms with the positions on the dispersion curves for bar waves, calculated with the SAFE procedure. However, numerous bar-wave modes prevented exact identification, especially above 500 kHz. Aperture effects contributed to the sensitivity reduction at higher frequencies and to more fluctuating bar-wave receiving sensitivities even for sensors with smooth or flat receiving sensitivities to normally incident waves. Spectral dips observed in bar-wave results can be accounted for by aperture effect predictions reasonably well. For the selection of AE sensors, one needs to use the appropriate type of sensitivities considering waves to be detected.
Neuroticism Predicts Subsequent Risk of Major Depression for Whites but Not Blacks
Authors: Assari Shervin
Published: September 2017

Cultural and ethnic differences in psychosocial and medical correlates of negative affect are well documented. This study aimed to compare blacks and whites for the predictive role of baseline neuroticism (N) on subsequent risk of major depressive episodes (MDD) 25 years later. Data came from the Americans’ Changing Lives (ACL) Study, 1986–2011. We used data on 1219 individuals (847 whites and 372 blacks) who had data on baseline N in 1986 and future MDD in 2011. The main predictor of interest was baseline N, measured using three items in 1986. The main outcome was 12 months MDD measured using the Composite International Diagnostic Interview (CIDI) at 2011. Covariates included baseline demographics (age and gender), socioeconomics (education and income), depressive symptoms [Center for Epidemiologic Studies Depression Scale (CES-D)], stress, health behaviors (smoking and driking), and physical health [chronic medical conditions, obesity, and self-rated health (SRH)] measured in 1986. Logistic regressions were used to test the predictive role of baseline N on subsequent risk of MDD 25 years later, net of covariates. The models were estimated in the pooled sample, as well as blacks and whites. In the pooled sample, baseline N predicted subsequent risk of MDD 25 years later (OR = 2.23, 95%CI = 1.14–4.34), net of covariates. We also found a marginally significant interaction between race and baseline N on subsequent risk of MDD (OR = 0.37, 95% CI = 0.12–1.12), suggesting a stronger effect for whites compared to blacks. In race-specific models, among whites (OR = 2.55; 95% CI = 1.22–5.32) but not blacks (OR = 0.90; 95% CI = 0.24–3.39), baseline N predicted subsequent risk of MDD. Black-white differences in socioeconomics and physical health could not explain the racial differences in the link between N and MDD. Blacks and whites differ in the salience of baseline N as a psychological determinant of MDD risk over a long period of time. This finding supports the cultural moderation hypothesis and is in line with other previously reported black–white differences in social, psychological, and medical correlates of negative affect and depression.
Energy, Exergy and Economic Evaluation Comparison of Small-Scale Single and Dual Pressure Organic Rankine Cycles Integrated with Low-Grade Heat Sources
Authors: Fontalvo Armando , Solano Jose , Pedraza Cristian , Bula Antonio , Gonzalez Quiroga Arturo , Vasquez Padilla Ricardo
Published: September 2017

Low-grade heat sources such as solar thermal, geothermal, exhaust gases and industrial waste heat are suitable alternatives for power generation which can be exploited by means of small-scale Organic Rankine Cycle (ORC). This paper combines thermodynamic optimization and economic analysis to assess the performance of single and dual pressure ORC operating with different organic fluids and targeting small-scale applications. Maximum power output is lower than 45 KW while the temperature of the heat source varies in the range 100–200 °C. The studied working fluids, namely R1234yf, R1234ze(E) and R1234ze(Z), are selected based on environmental, safety and thermal performance criteria. Levelized Cost of Electricity (LCOE) and Specific Investment Cost (SIC) for two operation conditions are presented: maximum power output and maximum thermal efficiency. Results showed that R1234ze(Z) achieves the highest net power output (up to 44 kW) when net power output is optimized. Regenerative ORC achieves the highest performance when thermal efficiency is optimized (up to 18%). Simple ORC is the most cost-effective among the studied cycle configurations, requiring a selling price of energy of 0.3 USD/kWh to obtain a payback period of 8 years. According to SIC results, the working fluid R1234ze(Z) exhibits great potential for simple ORC when compared to conventional R245fa.
Airflow Sensitivity Assessment Based on Underground Mine Ventilation Systems Modeling
Authors: Dziurzyński Wacław , Krach Andrzej , Pałka Teresa
Published: September 2017

This paper presents a method for determining the sensitivity of the main air flow directions in ventilation subnetworks to changes in aerodynamic resistance and air density in mine workings. The authors have developed formulae for determining the sensitivity of the main subnetwork air flows by establishing the degree of dependency of the air volume stream in a given working on the variations in resistance or air density of other workings of the network. They have been implemented in the Ventgraph mine ventilation network simulator. This software, widely used in Polish collieries, provides an extended possibility to predict the process of ventilation, air distribution and, in the case of underground fire, the spread of combustion gasses. The new method facilitates an assessment by mine ventilation services of the stability of ventilation systems in exploitation areas and determines the sensitivity of the main subnetwork air flow directions to changes in aerodynamic resistance and air density. Recently in some Polish collieries new longwalls are developed in seams located deeper than the bottom of the intake shaft. Such a solution is called “exploitation below the level of access” or “sublevel”. The new approach may be applied to such developments to assess the potential of changes in direction and air flow rates. In addition, an interpretation of the developed sensitivity indicator is presented. While analyzing air distributions for sublevel exploitation, the application of current numerical models for calculations of the distribution results in tangible benefits, such as the evaluation of the safety or risk levels for such exploitation. Application of the Ventgraph computer program, and particularly the module POŻAR (fire) with the newly developed options, allows for an additional approach to the sensitivity indicator in evaluating air flow safety levels for the risks present during exploitation below the level of the intake shaft. The analyses performed and examples presented enabled useful conclusions for mining practice to be drawn.
The Energy Footprint of China’s Textile Industry: Perspectives from Decoupling and Decomposition Analysis
Authors: Wang Laili , Li Yi , He Wanwen
Published: September 2017

Energy is the essential input for operations along the industrial manufacturing chain of textiles. China’s textile industry is facing great pressure on energy consumption reduction. This paper presents an analysis of the energy footprint (EFP) of China’s textile industry from 1991 to 2015. The relationship between EFP and economic growth in the textile industry was investigated with a decoupling index approach. The logarithmic mean Divisia index approach was applied for decomposition analysis on how changes in key factors influenced the EFP of China’s textile industry. Results showed that the EFP of China’s textile industry increased from 41.1 Mt in 1991 to 99.6 Mt in 2015. EFP increased fastest in the period of 1996–2007, with an average annual increasing rate of 7.7 percent, especially from 2001 to 2007 (8.5 percent). Manufacture of textile sector consumed most (from 58 percent to 76 percent) of the energy among the three sub-sectors, as it has lots of energy-intensive procedures. EFP and economic growth were in a relative decoupling state for most years of the researched period. Their relationship showed a clear tendency toward decoupling. Industrial scale was the most important factor that led to the increase of EFP, while decreasing energy intensity contributed significantly to reducing the EFP. The promoting effect of the factors was larger than the inhibiting effect on EFP in most years from 1991 to 2015.
Demand Response Unit Commitment Problem Solution for Maximizing Generating Companies’ Profit
Authors: Selvakumar K. , Vijayakumar K. , Boopathi C.
Published: September 2017

Over the recent years there has been an immense growth in load consumption due to which, Load Management (LM) has become more significant. Energy providers around the world apply different load management concepts and techniques to improve the load profile. In order to reduce the stress over the load management, Demand Response Unit Commitment (DRUC), a new concept, has been implemented in this paper. The main feature of this concept is that both the energy providers and consumers must participate in order to get mutual benefits hence maximizing each of their profits. In this paper we discuss the time-based Demand Response Program since there is no penalty observed in this program. When the Demand Response was combined with Unit Commitment and compiled it was observed that a satisfactory solution resulted, which is proved to be mutually beneficial for both Generating Companies (GENCOs) and their customers. Here, we have used a Cat Swarm Optimization (CSO) technique to find the solution for the DRUC problem. The results are obtained using CSO technique for UC problem with and without DR program. This is compared with the results obtained using other conventional methods. The test system considered for the study is IEEE39 bus system.
The Role of Body Adiposity Index in Determining Body Fat Percentage in Colombian Adults with Overweight or Obesity
Authors: Ramírez-Vélez Robinson , Correa-Bautista Jorge , González-Ruíz Katherine , Tordecilla-Sanders Alejandra , García-Hermoso Antonio , Schmidt-RioValle Jacqueline , González-Jiménez Emilio
Published: September 2017

The aim of this study is to investigate the accuracy of body adiposity index (BAI) as a convenient tool for assessing body fat percentage (BF%) in a sample of adults with overweight/obesity using bioelectrical impedance analysis (BIA). The study population was composed of 96 volunteers (60% female, mean age 40.6 ± 7.5 years old). Anthropometric characteristics (body mass index, height, waist-to-height ratio, hip and waist circumference), socioeconomic status, and diet were assessed, and BF% was measured by BIA-BF% and by BAI-BF%. Pearson’s correlation coefficient was used to evaluate the correlation between BAI-BF% and BF% assessed by BIA-BF%, while controlling for potential confounders. The concordance between the BF% measured by both methods was obtained with a paired sample t-test, Lin’s concordance correlation coefficient, and Bland-Altman plot analysis. Overall, the correlation between BF% obtained by BIA-BF% and estimated by BAI-BF% was r = 0.885, p < 0.001, after adjusting for potential confounders (age, socioeconomic status, and diet). Lin’s concordance correlation coefficient was moderate in both sexes. In the men, the paired t-test showed a significant mean difference in BF% between the methods (−5.6 (95%CI −6.4 to −4.8); p < 0.001). In the women, these differences were (−3.6 (95%CI −4.7 to −2.5); p < 0.001). Overall, the bias of the BAI-BF% was −4.8 ± 3.2 BF%; p < 0.001), indicating that the BAI-BF% method significantly underestimated the BF% in comparison with the reference method. In adults with overweight/obesity, the BAI presents low agreement with BF% measured by BIA-BF%; therefore, we conclude that BIA-BF% is not accurate in either sex when body fat percentage levels are low or high. Further studies are necessary to confirm our findings in different ethnic groups.
Tissue-Based MicroRNAs as Predictors of Biochemical Recurrence after Radical Prostatectomy: What Can We Learn from Past Studies?
Authors: Zhao Zhongwei , Stephan Carsten , Weickmann Sabine , Jung Monika , Kristiansen Glen , Jung Klaus
Published: September 2017

With the increasing understanding of the molecular mechanism of the microRNAs (miRNAs) in prostate cancer (PCa), the predictive potential of miRNAs has received more attention by clinicians and laboratory scientists. Compared with the traditional prognostic tools based on clinicopathological variables, including the prostate-specific antigen, miRNAs may be helpful novel molecular biomarkers of biochemical recurrence for a more accurate risk stratification of PCa patients after radical prostatectomy and may contribute to personalized treatment. Tissue samples from prostatectomy specimens are easily available for miRNA isolation. Numerous studies from different countries have investigated the role of tissue-miRNAs as independent predictors of disease recurrence, either alone or in combination with other clinicopathological factors. For this purpose, a PubMed search was performed for articles published between 2008 and 2017. We compiled a profile of dysregulated miRNAs as potential predictors of biochemical recurrence and discussed their current clinical relevance. Because of differences in analytics, insufficient power and the heterogeneity of studies, and different statistical evaluation methods, limited consistency in results was obvious. Prospective multi-institutional studies with larger sample sizes, harmonized analytics, well-structured external validations, and reasonable study designs are necessary to assess the real prognostic information of miRNAs, in combination with conventional clinicopathological factors, as predictors of biochemical recurrence.
Targeted Delivery of siRNA with pH-Responsive Hybrid Gold Nanostars for Cancer Treatment
Authors: Zhu Hongyan , Liu Wanwan , Cheng Ziting , Yao Ke , Yang Yu , Xu Bohui , Su Gaoxing
Published: September 2017

In this work, we report the engineering of gold nanostars (GNS) to deliver small interfering RNA (siRNA) into HepG2 cells. The ligand DG-PEG-Lipoic acid (LA)-Lys-9R (hydrazone) was designed to functionalize GNS, and create the nanoparticles named as 9R/DG-GNS (hydrazone). In the ligand, 2-deoxyglucose (DG) is the targeting molecule, polyethylene glycol (PEG) helps to improve the dispersity and biocompatibility, 9-poly-d-arginine (9R) is employed to provide a positive surface charge and adsorb negative siRNA, and hydrazone bonds are pH-responsive and can avoid receptor-mediated endosomal recycling. Compared to GNS alone, 9R/DG-GNS (hydrazone) showed superior transfection efficiency. The expressions of cyclooxygenase-2 (COX-2) in HepG2 and SGC7901 cells were significantly suppressed by siRNA/9R/DG-GNS (hydrazone) complex. Notably, 9R/DG-GNS (hydrazone) possessed low cytotoxicity even at high concentrations in both normal cells and tumor cells. The combination treatment of siRNA/9R/DG-GNS (hydrazone) complex inhibited the cell growth rate by more than 75%. These results verified that the pH-responsive GNS complex is a promising siRNA delivery system for cancer therapy, and it is anticipated that near-infrared absorbing GNS with good photothermal conversion efficiency can be potentially used for photothermal therapy of tumors.
Molecular Tools for the Detection and the Identification of Hymenoptera Parasitoids in Tortricid Fruit Pests
Authors: Franck Pierre , Maalouly-Matar Mariline , Olivares Jérôme
Published: September 2017

Biological control requires specific tools for the accurate detection and identification of natural enemies in order to estimate variations in their abundance and their impact according to changes in environmental conditions or agricultural practices. Here, we developed two molecular methods of detection based on PCR-RFLP with universal primers and on PCR with specific primers to identify commonly occurring larval parasitoids of the tortricid fruit pests and to estimate parasitism in the codling moth. Both methods were designed based on DNA sequences of the COI mitochondrial gene for a range of parasitoids that emerged from Cydia pomonella and Grapholita molesta caterpillars (102 parasitoids; nine species) and a range of potential tortricid hosts (40 moths; five species) damaging fruits. The PCR-RFLP method (digestion by AluI of a 482 bp COI fragment) was very powerful to identify parasitoid adults and their hosts, but failed to detect parasitoid larvae within eggs or within young C. pomonella caterpillars. The PCR method based on specific primers amplified COI fragments of different lengths (131 to 463 bp) for Ascogaster quadridentata (Braconidae); Pristomerus vulnerator (Ichneumonidae); Trichomma enecator (Ichneumonidae); and Perilampus tristis (Perilampidae), and demonstrated a higher level of sensibility than the PCR-RFLP method. Molecular estimations of parasitism levels in a natural C. pomonella population with the specific primers did not differ from traditional estimations based on caterpillar rearing (about 60% parasitism in a non-treated apple orchard). These PCR-based techniques provide information about within-host parasitoid assemblage in the codling moth and preliminary results on the larval parasitism of major tortricid fruit pests.
Spatiotemporal Control of Doxorubicin Delivery from “Stealth-Like” Prodrug Micelles
Authors: Kong Li , Poulcharidis Dimitrios , Schneider Gregory , Campbell Frederick , Kros Alexander
Published: September 2017

In the treatment of cancer, targeting of anticancer drugs to the tumor microenvironment is highly desirable. Not only does this imply accurate tumor targeting but also minimal drug release en route to the tumor and maximal drug release once there. Here we describe high-loading, “stealth-like” doxorubicin micelles as a pro-drug delivery system, which upon light activation, leads to burst-like doxorbicin release. Through this approach, we show precise spatiotemporal control of doxorubicin delivery to cells in vitro.
Proteome Stability as a Key Factor of Genome Integrity
Authors: Gumeni Sentiljana , Evangelakou Zoi , Gorgoulis Vassilis , Trougakos Ioannis
Published: September 2017

DNA damage is constantly produced by both endogenous and exogenous factors; DNA lesions then trigger the so-called DNA damaged response (DDR). This is a highly synchronized pathway that involves recognition, signaling and repair of the damage. Failure to eliminate DNA lesions is associated with genome instability, a driving force in tumorigenesis. Proteins carry out the vast majority of cellular functions and thus proteome quality control (PQC) is critical for the maintenance of cellular functionality. PQC is assured by the proteostasis network (PN), which under conditions of proteome instability address the triage decision of protein fold, hold, or degrade. Key components of the PN are the protein synthesis modules, the molecular chaperones and the two main degradation machineries, namely the autophagy-lysosome and the ubiquitin-proteasome pathways; also, part of the PN are a number of stress-responsive cellular sensors including (among others) heat shock factor 1 (Hsf1) and the nuclear factor erythroid 2-related factor 2 (Nrf2). Nevertheless, the lifestyle- and/or ageing-associated gradual accumulation of stressors results in increasingly damaged and unstable proteome due to accumulation of misfolded proteins and/or protein aggregates. This outcome may then increase genomic instability due to reduced fidelity in processes like DNA replication or repair leading to various age-related diseases including cancer. Herein, we review the role of proteostatic machineries in nuclear genome integrity and stability, as well as on DDR responses.
Investigation of the Spin Crossover Properties of Three Dinulear Fe(II) Triple Helicates by Variation of the Steric Nature of the Ligand Type
Authors: Craze Alexander , Sciortino Natasha , Badbhade Mohan , Kepert Cameron , Marjo Christopher , Li Feng
Published: September 2017

The investigation of new spin-crossover (SCO) compounds plays an important role in understanding the key design factors involved, informing the synthesis of materials for future applications in electronic and sensing devices. In this report, three bis-bidentate ligands were synthesized by Schiff base condensation of imidazole-4-carbaldehyde with 4,4-diaminodiphenylmethane (L1), 4,4′-diaminodiphenyl sulfide (L2) and 4,4′-diaminodiphenyl ether (L3) respectively. Their dinuclear Fe(II) triple helicates were obtained by complexation with Fe(BF4)2·6H2O in acetonitrile. The aim of this study was to examine the influence of the steric nature of the ligand central atom (–X–, where X = CH2, S or O) on the spin-crossover profile of the compound. The magnetic behaviours of these compounds were investigated and subsequently correlated to the structural information from single-crystal X-ray crystallographic experiments. All compounds [Fe2(L1)3](BF4)2 (1), [Fe2(L2)3](BF4)2 (2) and [Fe2(L3)3](BF4)2 (3), demonstrated approximately half-spin transitions, with T1/2 values of 155, 115 and 150 K respectively, corresponding to one high-spin (HS) and one low-spin (LS) Fe(II) centre in a [LS–HS] state at 50 K. This was also confirmed by crystallographic studies, for example, bond lengths and the octahedral distortion parameter (∑) at 100 K. The three-dimensional arrangement of the HS and LS Fe(II) centres throughout the crystal lattice was different for the three compounds, and differing extents of intermolecular interactions between BF4− counter ions and imidazole N–H were present. The three compounds displayed similar spin-transition profiles, with 2 (–S–) possessing the steepest nature. The shape of the spin transition can be altered in this manner, and this is likely due to the subtle effects that the steric nature of the central atom has on the crystal packing (and thus inter-helical Fe–Fe separation), intermolecular interactions and Fe–Fe intra-helical separations.
Markers of Microbial Translocation and Immune Activation Predict Cognitive Processing Speed in Heavy-Drinking Men Living with HIV
Authors: Monnig Mollie , Kahler Christopher , Cioe Patricia , Monti Peter , Mayer Kenneth , Pantalone David , Cohen Ronald , Ramratnam Bharat
Published: September 2017

HIV infection and alcohol use disorder are associated with deficits in neurocognitive function. Emerging evidence points to pro-inflammatory perturbations of the gut-brain axis as potentially contributing to neurocognitive impairment in the context of HIV and chronic heavy alcohol use. This study examined whether plasma markers of microbial translocation (LPS) from the gastrointestinal tract and related immune activation (sCD14, EndoCAb) were associated with neurocognition in 21 men living with HIV who were virally suppressed on antiretroviral therapy. All participants met federal criteria for heavy drinking and were enrolled in a randomized controlled trial (RCT) of a brief alcohol intervention. This secondary analysis utilized blood samples and cognitive scores (learning, memory, executive function, verbal fluency, and processing speed) obtained at baseline and three-month follow-up of the RCT. In generalized estimating equation models, LPS, sCD14, and EndoCAb individually were significant predictors of processing speed. In a model with all biomarkers, higher LPS and sCD14 both remained significant predictors of lower processing speed. These preliminary findings suggest that inflammation stemming from HIV and/or alcohol could have negative effects on the gut-brain axis, manifested as diminished processing speed. Associations of microbial translocation and immune activation with processing speed in heavy-drinking PLWH warrant further investigation in larger-scale studies.
Function Oriented Molecular Design: Dendrimers as Novel Antimicrobials
Authors: García-Gallego Sandra , Franci Gianluigi , Falanga Annarita , Gómez Rafael , Folliero Veronica , Galdiero Stefania , De la Mata Francisco , Galdiero Massimiliano
Published: September 2017

In recent years innovative nanostructures are attracting increasing interest and, among them, dendrimers have shown several fields of application. Dendrimers can be designed and modified in plentiful ways giving rise to hundreds of different molecules with specific characteristics and functionalities. Biomedicine is probably the field where these molecules find extraordinary applicability, and this is probably due to their multi-valency and to the fact that several other chemicals can be coupled to them to obtain desired compounds. In this review we will describe the different production strategies and the tools and technologies for the study of their characteristics. Finally, we provide a panoramic overview of their applications to meet biomedical needs, especially their use as novel antimicrobials.
Applications of PNA-Based Artificial Restriction DNA Cutters
Authors: Shigi Narumi , Sumaoka Jun , Komiyama Makoto
Published: September 2017

More than ten years ago, artificial restriction DNA cutters were developed by combining two pseudo-complementary peptide nucleic acid (pcPNA) strands with either Ce(IV)/EDTA or S1 nuclease. They have remarkably high site-specificity and can cut only one predetermined site in the human genome. In this article, recent progress of these man-made tools have been reviewed. By cutting the human genome site-selectively, desired fragments can be clipped from either the termini of chromosomes (telomeres) or from the middle of genome. These fragments should provide important information on the biological functions of complicated genome system. DNA/RNA hybrid duplexes, which are formed in living cells, are also site-selectively hydrolyzed by these cutters. In order to further facilitate the applications of the artificial DNA cutters, various chemical modifications have been attempted. One of the most important successes is preparation of PNA derivatives which can form double-duplex invasion complex even under high salt conditions. This is important for in vivo applications, since the inside of living cells is abundant of metal ions. Furthermore, site-selective DNA cutters which require only one PNA strand, in place of a pair of pcPNA strands, are developed. This progress has opened a way to new fields of PNA-based biochemistry and biotechnology.
Influence of Jiegeng on Pharmacokinetic Properties of Flavonoids and Saponins in Gancao
Authors: Mao Yancao , Peng Linxiu , Kang An , Xie Tong , Xu Jianya , Shen Cunsi , Ji Jianjian , Di Liuqing , Wu Hao , Shan Jinjun
Published: September 2017

Jiegeng Gancao decoction, which is composed of Jiegeng and Gancao at a weight ratio of 1:2, was widely used for treating pharyngalgia and cough for thousands of years. Our previous work indicated that Gancao could increase the systemic exposure of platycodin D and deapio-platycodin D, two main components in Jiegeng. However, whether Jiegeng could alter the pharmacokinetics of the main compounds in Gancao is still unknown. Thus, the purpose of this study was to compare the oral pharmacokinetics of flavonoids and saponins from Gancao alone vs. after co-administration with Jiegeng. Furthermore, Caco-2 cell transport and fecal hydrolysis were investigated to explain the altered pharmacokinetic properties. Pharmacokinetics results suggested that the bioavailability of liquiritin, isoliquiritin, glycyrrhizin and its metabolite, glycyrrhetinic acid, could be improved while bioavailability of liquiritigenin and isoliquiritigenin deteriorated when co-administered with Jiegeng. The Caco-2 transport study showed no significant difference of the Papp values of the main components in Jiegeng Gancao decoction when compared with those in Gancao decoction (p > 0.05). The in vitro metabolism study suggested that saponins and flavonoids glycosides in Gancao were influenced and the metabolic characteristics of most ingredients were consistent with pharmacokinetic results, such as liquiritin and glycyrrhetinic acid. The hydrolysis of liquiritigenin and glycyrrhizin observed with fecal lysate in vitro appeared consistent with the oral pharmacokinetics. Based on experiments, the pharmacokinetic profiles of six components in Gancao were influenced by Jiegeng. The metabolic process might partially contribute to the altered pharmacokinetic behavior. The metabolism of some components of Gancao appeared to be inhibited when coadministered with Jiegeng, possibly by the Jiegeng constituent platycodin.
Inhibitory Effect of Selaginellins from Selaginella tamariscina (Beauv.) Spring against Cytochrome P450 and Uridine 5′-Diphosphoglucuronosyltransferase Isoforms on Human Liver Microsomes
Authors: Heo Jae-Kyung , Nguyen Phi-Hung , Kim Won , Phuc Nguyen , Liu Kwang-Hyeon
Published: September 2017

Selaginella tamariscina (Beauv.) has been used for traditional herbal medicine for treatment of cancer, hepatitis, and diabetes in the Orient. Numerous bioactive compounds including alkaloids, flavonoids, lignans, and selaginellins have been identified in this medicinal plant. Among them, selaginellins having a quinone methide unit and an alkylphenol moiety have been known to possess anticancer, antidiabetic, and neuroprotective activity. Although there have been studies on the biological activities of selaginellins, their modulatory potential of cytochrome P450 (P450) and uridine 5′-diphosphoglucuronosyltransferase (UGT) activities have not been previously evaluated. In this study, we investigated the drug interaction potential of two selaginellins on ten P450 isoforms (CYP1A2, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 2J2 and 3A) and six UGT isoforms (UGT1A1, 1A3, 1A4, 1A6, 1A9 and 2B7) using human liver microsomes and liquid chromatography-tandem mass spectrometry. Selaginellin and selaginellin M had high inhibitory potential for CYP2C8-mediated amodiaquine O-demethylation with IC50 values of 0.5 and 0.9 μM, respectively. Selaginellin and selaginellin M also showed medium inhibitory potential against CYP2C9, CYP2J2, UGT1A1, and UGT1A3 (1 μM < IC50 < 5 μM). These two selaginellins had low inhibitory potential against CYP1A2, CYP2A6, CYP2E1, and UGT1A6 (IC50 > 25 μM). This information might be helpful to predict possible drug interaction potential of between selaginellins and co-administered drugs.
Parallel Implementation of the CCSDS 1.2.3 Standard for Hyperspectral Lossless Compression
Authors: Báscones Daniel , González Carlos , Mozos Daniel
Published: September 2017

Hyperspectral imaging is a technology which, by sensing hundreds of wavelengths per pixel, enables fine studies of the captured objects. This produces great amounts of data that require equally big storage, and compression with algorithms such as the Consultative Committee for Space Data Systems (CCSDS) 1.2.3 standard is a must. However, the speed of this lossless compression algorithm is not enough in some real-time scenarios if we use a single-core processor. This is where architectures such as Field Programmable Gate Arrays (FPGAs) and Graphics Processing Units (GPUs) can shine best. In this paper, we present both FPGA and OpenCL implementations of the CCSDS 1.2.3 algorithm. The proposed paralellization method has been implemented on the Virtex-7 XC7VX690T, Virtex-5 XQR5VFX130 and Virtex-4 XC2VFX60 FPGAs, and on the GT440 and GT610 GPUs, and tested using hyperspectral data from NASA’s Airborne Visible Infra-Red Imaging Spectrometer (AVIRIS). Both approaches fulfill our real-time requirements. This paper attempts to shed some light on the comparison between both approaches, including other works from existing literature, explaining the trade-offs of each one.
ROSA: Resource-Oriented Service Management Schemes for Web of Things in a Smart Home
Authors: Liao Chun-Feng , Chen Peng-Yu
Published: September 2017

A Pervasive-computing-enriched smart home environment, which contains many embedded and tiny intelligent devices and sensors coordinated by service management mechanisms, is capable of anticipating intentions of occupants and providing appropriate services accordingly. Although there are a wealth of research achievements in recent years, the degree of market acceptance is still low. The main reason is that most of the devices and services in such environments depend on particular platform or technology, making it hard to develop an application by composing the devices or services. Meanwhile, the concept of Web of Things (WoT) is becoming popular recently. Based on WoT, the developers can build applications based on popular web tools or technologies. Consequently, the objective of this paper is to propose a set of novel WoT-driven plug-and-play service management schemes for a smart home called Resource-Oriented Service Administration (ROSA). We have implemented an application prototype, and experiments are performed to show the effectiveness of the proposed approach. The results of this research can be a foundation for realizing the vision of “end user programmable smart environments”.
Graphene-Based Materials for Biosensors: A Review
Authors: Suvarnaphaet Phitsini , Pechprasarn Suejit
Published: September 2017

The advantages conferred by the physical, optical and electrochemical properties of graphene-based nanomaterials have contributed to the current variety of ultrasensitive and selective biosensor devices. In this review, we present the points of view on the intrinsic properties of graphene and its surface engineering concerned with the transduction mechanisms in biosensing applications. We explain practical synthesis techniques along with prospective properties of the graphene-based materials, which include the pristine graphene and functionalized graphene (i.e., graphene oxide (GO), reduced graphene oxide (RGO) and graphene quantum dot (GQD). The biosensing mechanisms based on the utilization of the charge interactions with biomolecules and/or nanoparticle interactions and sensing platforms are also discussed, and the importance of surface functionalization in recent up-to-date biosensors for biological and medical applications.