bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Economic Awareness on Sustainable Energy Consumption: Results of Research in a Segment of Polish Households
Authors: Gajdzik Bożena , Jaciow Magdalena , Hoffmann-Burdzińska Kinga , Wolny Robert , Wolniak Radosław , Grebski Wiesław Wes
Published: May 2024

This manuscript explores the relationship between the economic awareness (as a part of energy awareness) of Polish households and their sustainable energy consumption practices. Sustainable consumption is measured by the frequency of behaviors such as turning off electrical devices when not in use, removing mobile device chargers from sockets, switching off lights when leaving a room, preferring showers over baths, using washing machines and dishwashers only when full, and purchasing energy-efficient appliances and light bulbs. Economic awareness is gauged through variables such as knowledge of electricity tariffs, understanding of electric bill components, awareness of electricity prices, exact knowledge of electricity expenses, electricity usage in kWh, knowledge of effective energy-saving methods, and familiarity with the energy efficiency classes of appliances and light bulbs. This study presents profiles of households with high and low economic awareness regarding their electricity expenditures and examines how these profiles differ in their sustainable energy consumption behaviors. This research is based on a survey of 1407 Polish households conducted online in 2023. Data collected from the survey were subjected to statistical analysis and are presented in tables and graphs. The findings are discussed in the context of the existing literature in the field, highlighting the implications of economic awareness on sustainable energy consumption practices. This research contributes to understanding how economic knowledge influences energy-saving behaviors among Polish households, providing insights for policymakers and energy conservation initiatives. One of the key findings of this paper is the significant association between economic awareness, energy-saving knowledge, and the adoption of sustainable energy consumption behaviors among Polish households. This study reveals that households with higher levels of economic awareness demonstrate a notably higher frequency of practices related to sustainable energy consumption compared to those with lower economic awareness. Similarly, households equipped with greater knowledge about energy-saving techniques exhibit a higher propensity to adopt energy-efficient behaviors. This underscores important roles of economic literacy and education in fostering behavioral changes towards more sustainable energy practices, highlighting the importance of targeted interventions and educational campaigns aimed at enhancing economic awareness and promoting energy-saving knowledge among consumers.
Enabling Passive Load-Holding Function and System Pressures Control in a One-Motor-One-Pump Motor-Controlled Hydraulic Cylinder: Simulation Study
Authors: Zhao Wei , Ebbesen Morten Kjeld , Hansen Michael Rygaard , Andersen Torben Ole
Published: May 2024

This paper is concerned with a hydraulic cylinder directly controlled by a variable speed motor-driven fixed-displacement pump. The considered configuration is referred to as a one-motor-one-pump (1M1P) motor-controlled hydraulic cylinder (MCC). A 1M1P MCC with a hydraulically driven passive load-holding function enabled by controlled system pressures is proposed. The proposed load-holding functionality works if there is a standstill command, a loss of power supply, or a hose rupture. Additionally, this paper conducts a comprehensive analysis of the proposed system’s operation and load-holding function across four quadrants. The simulation results demonstrate four-quadrant operation and good load-holding performance under the aforementioned scenarios. In conclusion, the proposed 1M1P MCC can be successfully used on practical applications characterized by overrunning loads and four quadrant operation.
Impact of Wave Energy Converters and Port Layout on Coastal Dynamics: Case Study of Astara Port
Authors: Moradi Mehrdad , Ilinca Adrian
Published: May 2024

In the face of depleting fossil energy and the imperative of sustainable development, there is a compelling drive towards advancing renewable energies. In this context, sustainable and predictable alternatives, like marine energy, gain prominence. Marine energy presents a cleaner option devoid of the adverse effects associated with fossil fuels, playing a crucial role in environmental sustainability by safeguarding coastlines against erosion. This study focuses on Astara Port in the Caspian Sea, exploring the utilization of wave energy converters (WECs). The originality of this study’s research lies in exploring WECs’ dual role in energy generation and coastal protection. Using MIKE21 software simulations, the impact of number, location, arrangement, and orientation of WECs across various scenarios was investigated, including two WEC number scenarios (11 and 13), three structural placement scenarios (north, front, and south of the port), two structural arrangement scenarios (linear and staggered), two port layout scenarios (original layout and modified layout), and two orientation scenarios for the structures (facing north-east, which is the dominant wave direction, and facing southeast). The results show a remarkable decrease in the significant wave height behind WECs, notably with 13 staggered devices facing dominant waves (from northeast), reducing the significant wave height Hs by 23–25%. This setup also shows the highest wave height reduction, notably 36.26% during a storm event. However, linear WEC setup offers more extensive coastline protection, covering 47.88% of the model boundary during storms. Furthermore, the 11 staggered WECs facing southeast (SE) arrangement had the lowest sediment accumulation at 0.0358 m over one year, showing effective sedimentation mitigation potential. Conversely, the 13 linear WECs facing northeast (NE) had the highest accumulation at 0.1231 m. Finally, the proposed port design redirects high-velocity flow away from the port entrance and removes rotatory flow, reducing sediment accumulation near the harbor entrance.
Single Three-Phase Inverter for Dual-Frequency Induction Heating
Authors: Frania Krystian , Kierepka Kamil , Kasprzak Marcin , Zimoch Piotr
Published: May 2024

This paper presents new resonant inverter topologies for dual-frequency induction heating (IH). These 2T1C and 3T topologies combine the advantageous features of two- and one-inverter solutions. An analytical description of the load impedance of a dual-frequency series-parallel circuit has been made. Using manufacturer datasheets and LTspice models of selected transistors, a MATLAB model was parameterized. Based on it, power losses are determined as a function of the following parameters: nominal power, frequency and DC bus voltage. The obtained results allowed for determining the data necessary in the design process. The research has been experimentally verified. Tests were carried out for pulsing and simultaneous operation. Power control characteristics as a function of frequency are determined. The possibility of operating the inverter with high efficiency (>97%) in the proposed 2T1C and 3T systems at nominal power is demonstrated.
Energy Analysis of Waste Heat Recovery Using Supercritical CO2 Brayton Cycle for Series Hybrid Electric Vehicles
Authors: Mocanu Gabriel , Iosifescu Cristian , Ion Ion V. , Popescu Florin , Frătița Michael , Chivu Robert Mădălin
Published: May 2024

Waste heat recovery from exhaust gas is one of the most convenient methods to save energy in internal combustion engine-driven vehicles. This paper aims to investigate a reduction in waste heat from the exhaust gas of an internal combustion engine of a serial Diesel–electric hybrid bus by recovering part of the heat and converting it into useful power with the help of a split-flow supercritical CO2 (sCO2) recompression Brayton cycle. It can recover 17.01 kW of the total 33.47 kW of waste heat contained in exhaust gas from a 151 kW internal combustion engine. The thermal efficiency of the cycle is 38.51%, and the net power of the cycle is 6.55 kW. The variation in the sCO2 temperature at the shutdown of the internal combustion engine is analyzed, and a slow drop followed by a sudden and then a slow drop is observed. After 80 s from stopping the engine, the temperature drops by (23–33)% depending on the tube thickness of the recovery heat exchanger. The performances (net power, thermal efficiency, and waste heat recovery efficiency) of the split-flow sCO2 recompression Brayton cycle are clearly superior to those of the steam Rankine cycle and the organic Rankine cycle (ORC) with cyclopentane as a working fluid.
The Impacts of Tree Species on Soil Properties in Afforested Areas: A Case Study in Central Subtropical China
Authors: Hu Miao , Wang Yiping , Li Huihu , Hu Liping , Liu Qiaoli , Zhou Fan , Yang Aihong , Yu Faxin , Ouyang Xunzhi
Published: May 2024

Afforestation plays a critical role in ecosystem restoration and carbon sequestration. However, there continues to be insufficient knowledge about the long-term effects of different tree species on the forest soil in central subtropical China. In this study, five indigenous afforestation tree species commonly used in the region, including Bretschneidera sinensis, Liriodendron chinense, Schima superba, Phoebe bournei, and Cunninghamia lanceolata, were selected to explore their long-term effects on the forest soil. The soil’s physicochemical properties, organic carbon content, enzyme activity, and respiration were investigated. Our results revealed significant differences in the soil physicochemical properties, enzyme activity, organic carbon content, and soil respiration among the different tree species even with the same tree species types. Broad-leaved species, particularly L. chinense and P. bournei, exhibited superior soil physicochemical properties, higher amounts of organic carbon contents, enzyme activity, and soil respiration compared to coniferous species C. lanceolata. Notably, for the two studied evergreen tree species, P. bournei seemed to improve the forest soil quality more than S. superba. Hence, increasing the proportion of broad-leaved tree species may have a beneficial effect on the soil’s physicochemical properties and microecology. Furthermore, considering tree species’ compositions in afforestation will help to optimize soil quality and ecosystem health.
Effect of Combined Factors on Moth Communities in Western Hungarian Sessile Oak–Hornbeam Forests
Authors: Horváth Bálint , Tóth Viktória , Bolla Bence , Szabóky Csaba , Eötvös Csaba Béla
Published: May 2024

The many publications on forests and moth communities accomplished in different sampling regions and habitat types have produced diverse results and conclusions. The multiplicity of outcomes requires regional or local investigations on forest traits and herbivores to determine optimal management methods to maintain biodiversity and ecological stability in woodlands. Our study focused on sessile oak–hornbeam forests, which are economically and ecologically significant in many European countries. Samplings were performed in 2011–2012 using portable light traps in a highly forested area in western Hungary. We used 16 variables for PCA from the sampling of vascular plants and the local forest management plan document. These newly created variables (i.e., principal components) were related (used generalized linear models) to different groups of sampled moth communities: Macrolepidoptera, Microlepidoptera, and ecological groups (according to the host vegetation layer). Based on these significant relations, thinning activity may have various effects on moth communities, through the changed light regime and microclimate conditions. Temperature growth in the gaps could lead to the increasing abundance of heat-preferred Lepidoptera species; however, the decreasing species richness of trees (as a result of thinning) is less favourable for moth assemblages. Increasing herb coverage supports moth communities in the investigated forest stands, which may also be induced by the lower canopy closure. Besides the increasing coverage in the lower vegetation layers, plant species richness is also an important element for moth communities; this was demonstrated by the negative relation between the PC4, PC6 (weighted toward coverage), and Lepidoptera groups. Our results supported the fact that a single study on forest management practice or on vegetation traits is not sufficient to indicate their exact effect on moth communities, because their influence is complex. In order to halt the loss in diversity of the examined forest type, we suggest an overall approach to define the optimal forest management practice and tree mixture rate, regarding a larger area.
Effects of Slope Position on the Rhizosphere and Fine Root Microbiomes of Cupressus gigantea on the Tibet Plateau, China
Authors: Gong Wenfeng , Wei Liping , Liu Jinliang
Published: May 2024

Cupressus gigantea is an endangered species mainly distributed on beach land, down-slope, and middle-slope positions along the Yarlung Zangbo River on the Tibet Plateau of China, with an altitude ranging from 3000 to 3400 m. We investigated the rhizosphere and fine root microbiomes of C. gigantea at these three slope positions through metagenomic analysis. Slope positions had a greater influence on microbiome composition in the rhizosphere than that in the fine roots. Down- and middle-slope positions presented higher microbial richness indeces and community similarity, while a more complex co-occurrence network was observed in the beach land samples. Rhizosphere bacterial community assembly was determined via deterministic processes in the beach land and via stochastic processes in the down- and middle-slope positions. Archaeal and fungal community assemblies were both dominated by stochastic processes in the rhizosphere and fine roots at the three slope positions. Nitrogen (N) functional genes were more sensitive to changes in slope positions than phosphorus (N) functional genes. Soil properties explained more than 60% and 34% of the variations in the N and P functional genes and more than 30% and 10% of the variations in the microbiomes in the rhizosphere and fine roots, respectively. Variation in the microbiome was significantly driven by total nirtogen, total potassium, pH, and soil moisture in rhizosphere, and by pH and soil moisture in fine roots. Our observations suggest that the effect of slope position on the microbiomes of C. gigantea was greater for the rhizosphere than the fine roots, with down- and middle-slope positions presenting higher community similarity.
Effect of Stocking Density on Survival and Growth of Brown Meagre Sciaena umbra (L.) Larvae
Authors: Millot Rémi , Demolliens Mikaël , Filippi Jean-José , Pugliese Sally , Delmas Alban , Boussard Alizée , Alonso Loriane , Suchel Adeline , Poletti Emilie , Luca Jean-François , Hayoun Reda , Gutierrez Laura , Vela Alexandre , Bastien Romain , Aiello Antoine
Published: May 2024

Successful aquacultural production of juvenile fish requires that the optimal rearing conditions be applied. However, for many fish species, there is a significant knowledge gap concerning these conditions. In this study, we evaluated the effects of stocking density on the survival and growth of brown meagre, Sciaena umbra (L.), during rearing trials in an experimental hatchery. This research forms part of a feasibility project to improve the aquacultural production of brown meagre. Four initial larval density treatments (5, 10, 30, and 70 larvae per L) were established. At 0, 9, 17, 22, and 25 days post-hatching, we measured the total length, coefficient of variation in length, and specific growth rate. The physicochemical water parameters remained at satisfactory levels for fish culture throughout the experiment. Lower densities promoted better growth in terms of total length, specific growth rate, and survival. We observed a significant negative correlation between larval density and length growth/survival. Thus, the low larval density treatment yielded the highest survival (48.5% ± 3.46%), growth in final total length (11.9 ± 1.09 mm), and specific growth rate (5.13% ± 0.39% per day). Increased stocking density therefore negatively affects growth and survival, reduces homogeneity, but increases the production of S. umbra larvae. This study helps identify optimal aquaculture conditions for maximizing the production of juvenile S. umbra for ecological restoration.
Effect of Germination on Seed Protein Quality and Secondary Metabolites and Potential Modulation by Pulsed Electric Field Treatment
Authors: Bagarinao Norma Cecille , King Jessie , Leong Sze Ying , Agyei Dominic , Sutton Kevin , Oey Indrawati
Published: May 2024

Plant-based foods are being increasingly favored to feed the ever-growing population, but these need to exhibit improved nutritional value in terms of protein quality and digestibility to be considered a useful alternative to animal-based foods. Germination is essential for plant growth and represents a viable method through which the protein quality of plants can be further improved. However, it will be a challenge to maintain efficient rates of germination in a changing climate when seeds are sown. In the context of the indoor germination of seeds for food, consumption, or processing purposes, a more efficient and sustainable process is desired. Therefore, novel techniques to facilitate seed germination are required. Pulsed electric fields (PEF) treatment of seeds results in the permeabilization of the cell membrane, allowing water to be taken up more quickly and triggering biochemical changes to the macromolecules in the seed during germination. Therefore, PEF could be a chemical-free approach to induce a stress response in seeds, leading to the production of secondary metabolites known to exert beneficial effects on human health. However, this application of PEF, though promising, requires further research to optimize its impact on the protein and bioactive compounds in germinating seeds.
Comprehensive Evaluation of the Physicochemical Attributes, Antioxidant Capacity, and pH-Responsive Behavior of Starch Films Enhanced by Laver Incorporation
Authors: Chen Ying , Zhu Zhu , Ye Yunyue , Li Qi , Yang Tao , Guan Chengran , Liu Fengsong
Published: May 2024

Herein, a new starch film incorporating laver was developed to address issues related to inadequate water resistance and suboptimal preservation quality in food packaging. The integration of laver into starch film formulations offers a compelling avenue for creating biodegradable, active, and smart food packaging. Scanning electron microscope (SEM) analysis revealed that the starch film with a laver concentration of 70% exhibited a uniformly flat microstructure, as expected. Fourier-transform infrared spectroscopy (FTIR) confirmed the presence of intermolecular interactions and hydrogen bonding between the starch and laver. Viscoelastic tests demonstrated the superior film-forming performance of the starch/laver composite films. Moreover, it was found that the most favorable concentration of incorporated laver was 10%. Specifically, the S7-3 film emerged as a promising candidate for food packaging applications, boasting the highest contact angle (CA) value of 114.98 ± 1.28°, the lowest water solubility (WS) value of 15.38%, and a reduced water vapor transmission rate (WVTR) value of 2.52 g/m2 × h. Additionally, the S3-7 film displayed an extraordinary tensile strength of 32.47 MPa, an elongation at break of 19.04%, and a Young’s modulus of 606.83 MPa. Furthermore, the starch/laver composite films exhibited outstanding UV-blocking capabilities, exceptional pH-responsive behavior, and significant antioxidant activity, underscoring their potential for packaging applications with laver integration.
The Effect of Nanoscale Modification of Nisin by Different Milk-Derived Proteins on Its Physicochemical Properties and Antibacterial Activity
Authors: Wang Jing , Liu Rui , Huang Xiaoyang , Bao Yuexin , Wang Xiaohong , Yi Huaxi , Lu Youyou
Published: May 2024

Nisin is used as a natural food preservative because of its broad-spectrum antimicrobial activity against Gram-positive bacteria. However, free nisin is susceptible to various factors that reduce its antimicrobial activity. Milk protein, a protein derived from milk, has self-assembly properties and is a good carrier of bioactive substances. In this study, lactoferrin–nisin nanoparticles (L-N), bovine serum albumin–nisin nanoparticles (B-N), and casein–nisin nanoparticles (C-N) were successfully prepared by a self-assembly technique, and then their properties were investigated. The studies revealed that lactoferrin (LF) and nisin formed L-N mainly through hydrophobic interactions and hydrogen bonding, and L-N had the best performance. The small particle size (29.83 ± 2.42 nm), dense reticular structure, and good thermal stability, storage stability, and emulsification of L-N laid a certain foundation for its application in food. Further bacteriostatic studies showed that L-N enhanced the bacteriostatic activity of nisin, with prominent inhibitory properties against Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus, which mainly disrupted the cell membrane of the bacteria. The above results broaden our understanding of milk protein–nisin nanoparticles, while the excellent antibacterial activity of L-N makes it promising for application as a novel food preservative, which will help to improve the bioavailability of nisin in food systems.
Exploring the Interplay of Food Security, Safety, and Psychological Wellness in the COVID-19 Era: Managing Strategies for Resilience and Adaptation
Authors: Zhou Fanrui , Ma Zhengxin , Rashwan Ahmed K. , Khaskheli Muhammad Bilawal , Abdelrady Wessam A. , Abdelaty Nesma S. , Hassan Askri Syed Muhammad , Zhao Ping , Chen Wei , Shamsi Imran Haider
Published: May 2024

The global population surge presents a dual challenge and opportunity in the realms of food consumption, safety, and mental well-being. This necessitates a projected 70% increase in food production to meet growing demands. Amid this backdrop, the ongoing COVID-19 pandemic exacerbates these issues, underscoring the need for a deeper understanding of the intricate interplay between food consumption patterns and mental health dynamics during this crisis. Mitigating the spread of COVID-19 hinges upon rigorous adherence to personal hygiene practices and heightened disease awareness. Furthermore, maintaining stringent food quality and safety standards across both public and private sectors is imperative for safeguarding public health and containing viral transmission. Drawing upon existing research, this study delves into the pandemic’s impact on mental health, food consumption habits, and food safety protocols. Through a comprehensive analysis, it aims to elucidate the nuanced relationship among food, food safety, and mental well-being amid the COVID-19 pandemic, highlighting synergistic effects and dynamics that underpin holistic human welfare. Our study offers a novel approach by integrating psychological wellness with food security and safety. In conceiving this review, we aimed to comprehensively explore the intricate interplay among food security, safety, and psychological wellness amid the backdrop of the COVID-19 pandemic. Our review is structured to encompass a thorough examination of existing research, synthesizing insights into the multifaceted relationships among food consumption patterns, mental health dynamics, and food safety protocols during the crisis. Our findings provide valuable insights and practical recommendations for enhancing food security and psychological well-being, thus supporting both academic research and real-world applications in crisis management and policy development.
NIR Sensing Technologies for the Detection of Fraud in Nuts and Nut Products: A Review
Authors: Vega-Castellote Miguel , Sánchez María-Teresa , Torres-Rodríguez Irina , Entrenas José-Antonio , Pérez-Marín Dolores
Published: May 2024

Food fraud is a major threat to the integrity of the nut supply chain. Strategies using a wide range of analytical techniques have been developed over the past few years to detect fraud and to assure the quality, safety, and authenticity of nut products. However, most of these techniques present the limitations of being slow and destructive and entailing a high cost per analysis. Nevertheless, near-infrared (NIR) spectroscopy and NIR imaging techniques represent a suitable non-destructive alternative to prevent fraud in the nut industry with the advantages of a high throughput and low cost per analysis. This review collects and includes all major findings of all of the published studies focused on the application of NIR spectroscopy and NIR imaging technologies to detect fraud in the nut supply chain from 2018 onwards. The results suggest that NIR spectroscopy and NIR imaging are suitable technologies to detect the main types of fraud in nuts.
New Multiplicity Results for a Boundary Value Problem Involving a ψ-Caputo Fractional Derivative of a Function with Respect to Another Function
Authors: Li Yankai , Li Dongping , Chen Fangqi , Liu Xiangjing
Published: May 2024

This paper considers a nonlinear impulsive fractional boundary value problem, which involves a ψ-Caputo-type fractional derivative and integral. Combining critical point theory and fractional calculus properties, such as the semigroup laws, and relationships between the fractional integration and differentiation, new multiplicity results of infinitely many solutions are established depending on some simple algebraic conditions. Finally, examples are also presented, which show that Caputo-type fractional models can be more accurate by selecting different kernels for the fractional integral and derivative.
PIWI-Interacting RNAs: A Pivotal Regulator in Neurological Development and Disease
Authors: Pan Xian , Dai Wang , Wang Zhenzhen , Li Siqi , Sun Tao , Miao Nan
Published: May 2024

PIWI-interacting RNAs (piRNAs), a class of small non-coding RNAs (sncRNAs) with 24–32 nucleotides (nt), were initially identified in the reproductive system. Unlike microRNAs (miRNAs) or small interfering RNAs (siRNAs), piRNAs normally guide P-element-induced wimpy testis protein (PIWI) families to slice extensively complementary transposon transcripts without the seed pairing. Numerous studies have shown that piRNAs are abundantly expressed in the brain, and many of them are aberrantly regulated in central neural system (CNS) disorders. However, the role of piRNAs in the related developmental and pathological processes is unclear. The elucidation of piRNAs/PIWI would greatly improve the understanding of CNS development and ultimately lead to novel strategies to treat neural diseases. In this review, we summarized the relevant structure, properties, and databases of piRNAs and their functional roles in neural development and degenerative disorders. We hope that future studies of these piRNAs will facilitate the development of RNA-based therapeutics for CNS disorders.
The Phenotype-Based Approach Can Solve Cold Cases: The Paradigm of Mosaic Mutations of the CREBBP Gene
Authors: Marchetti Giulia Bruna , Milani Donatella , Pisciotta Livia , Pezzoli Laura , Marchisio Paola , Rinaldi Berardo , Iascone Maria
Published: May 2024

Rubinstein–Taybi syndrome (RTS) is a rare genetic disorder characterized by intellectual disability, facial dysmorphisms, and enlarged thumbs and halluces. Approximately 55% of RTS cases result from pathogenic variants in the CREBBP gene, with an additional 8% linked to the EP300 gene. Given the close relationship between these two genes and their involvement in epigenomic modulation, RTS is grouped into chromatinopathies. The extensive clinical heterogeneity observed in RTS, coupled with the growing number of disorders involving the epigenetic machinery, poses a challenge to a phenotype-based diagnostic approach for these conditions. Here, we describe the first case of a patient clinically diagnosed with RTS with a CREBBP truncating variant in mosaic form. We also review previously described cases of mosaicism in CREBBP and apply clinical diagnostic guidelines to these patients, confirming the good specificity of the consensus. Nonetheless, these reports raise questions about the potential underdiagnosis of milder cases of RTS. The application of a targeted phenotype-based approach, coupled with high-depth NGS, may enhance the diagnostic yield of whole-exome sequencing (WES) in mild and mosaic conditions.
Transcriptional Regulation Analysis Provides Insight into the Function of GSK3β Gene in Diannan Small-Ear Pig Spermatogenesis
Authors: Zhang Xia , Zhao Guiying , Yang Fuhua , Li Changyao , Lin Wan , Dai Hongmei , Zhai Lan , Xi Xuemin , Yuan Qingting , Huo Jinlong
Published: May 2024

Glycogen synthase kinase-3β (GSK3β) not only plays a crucial role in regulating sperm maturation but also is pivotal in orchestrating the acrosome reaction. Here, we integrated single-molecule long-read and short-read sequencing to comprehensively examine GSK3β expression patterns in adult Diannan small-ear pig (DSE) testes. We identified the most important transcript ENSSSCT00000039364 of GSK3β, obtaining its full-length coding sequence (CDS) spanning 1263 bp. Gene structure analysis located GSK3β on pig chromosome 13 with 12 exons. Protein structure analysis reflected that GSK3β consisted of 420 amino acids containing PKc-like conserved domains. Phylogenetic analysis underscored the evolutionary conservation and homology of GSK3β across different mammalian species. The evaluation of the protein interaction network, KEGG, and GO pathways implied that GSK3β interacted with 50 proteins, predominantly involved in the Wnt signaling pathway, papillomavirus infection, hippo signaling pathway, hepatocellular carcinoma, gastric cancer, colorectal cancer, breast cancer, endometrial cancer, basal cell carcinoma, and Alzheimer’s disease. Functional annotation identified that GSK3β was involved in thirteen GOs, including six molecular functions and seven biological processes. ceRNA network analysis suggested that DSE GSK3β was regulated by 11 miRNA targets. Furthermore, qPCR expression analysis across 15 tissues highlighted that GSK3β was highly expressed in the testis. Subcellular localization analysis indicated that the majority of the GSK3β protein was located in the cytoplasm of ST (swine testis) cells, with a small amount detected in the nucleus. Overall, our findings shed new light on GSK3β’s role in DSE reproduction, providing a foundation for further functional studies of GSK3β function.
Orthologs at the Base of the Olfactores Clade
Authors: Stein Wilfred D.
Published: May 2024

Tunicate orthologs in the human genome comprise just 84 genes of the 19,872 protein-coding genes and 23 of the 16,528 non-coding genes, yet they stand at the base of the Olfactores clade, which radiated to generate thousands of tunicate and vertebrate species. What were the powerful drivers among these genes that enabled this process? Many of these orthologs are present in gene families. We discuss the biological role of each family and the orthologs’ quantitative contribution to the family. Most important was the evolution of a second type of cadherin. This, a Type II cadherin, had the property of detaching the cell containing that cadherin from cells that expressed the Type I class. The set of such Type II cadherins could now detach and move away from their Type I neighbours, a process which would eventually evolve into the formation of the neural crest, “the fourth germ layer”, providing a wide range of possibilities for further evolutionary invention. A second important contribution were key additions to the broad development of the muscle and nerve protein and visual perception toolkits. These developments in mobility and vision provided the basis for the development of the efficient predatory capabilities of the Vertebrata.
Hypoxia in the Blue Mussel Mytilus chilensis Induces a Transcriptome Shift Associated with Endoplasmic Reticulum Stress, Metabolism, and Immune Response
Authors: Montúfar-Romero Milton , Valenzuela-Muñoz Valentina , Valenzuela-Miranda Diego , Gallardo-Escárate Cristian
Published: May 2024

The increase in hypoxia events, a result of climate change in coastal and fjord ecosystems, impacts the health and survival of mussels. These organisms deploy physiological and molecular responses as an adaptive mechanism to maintain cellular homeostasis under environmental stress. However, the specific effects of hypoxia on mussels of socioeconomic interest, such as Mytilus chilensis, are unknown. Using RNA-seq, we investigated the transcriptomic profiles of the gills, digestive gland, and adductor muscle of M. chilensis under hypoxia (10 days at 2 mg L−1) and reoxygenation (10 days at 6 mg L−1). There were 15,056 differentially expressed transcripts identified in gills, 11,864 in the digestive gland, and 9862 in the adductor muscle. The response varied among tissues, showing chromosomal changes in Chr1, Chr9, and Chr10 during hypoxia. Hypoxia regulated signaling genes in the Toll-like, mTOR, citrate cycle, and apoptosis pathways in gills, indicating metabolic and immunological alterations. These changes suggest that hypoxia induced a metabolic shift in mussels, reducing reliance on aerobic respiration and increasing reliance on anaerobic metabolism. Furthermore, hypoxia appeared to suppress the immune response, potentially increasing disease susceptibility, with negative implications for the mussel culture industry and natural bed populations. This study provides pivotal insights into metabolic and immunological adaptations to hypoxia in M. chilensis, offering candidate genes for adaptive traits.
Humility and Perspective-Taking: Ford’s Ethics and Aesthetics of War Writing
Authors: Sorum Eve
Published: May 2024

In an essay written sometime in 1917 or 1918, unpublished during his lifetime and only discovered in 1980, Ford Madox Ford reflects on what his war experience in France taught him: “above all things—humility”. This article argues that Ford’s writing about humility and perspective-taking in his wartime essays, which he connects to unstinting attentiveness to the particularities of place and people, can be read through an ecocritical lens that sees an ecological humility as central to reorienting human relationships within the natural world. In reflecting on both the lessons of war and the causes of such conflicts, Ford highlights humility in terms of perspective-taking and, in a related move, foregrounds the necessity for the precise use of language—both he sees as key to representing and preventing war. In so doing, I argue, Ford calls for an aesthetics and an ethics of war writing. Such literature must realize the impossibility and hubris of the bird’s-eye view, instead rooting itself in the ground, both literally and linguistically, while using a precise language that emerges from a clear awareness of this limited perspective.
How Climate Variability Affects Safflower (Carthamus tinctorius L.) Yield, Oil, and Fatty Acids in Response to Sowing Dates
Authors: Sajid Muhammad , Munir Hassan , Rauf Saeed , Ibtahaj Iqra , Paray Bilal Ahamad , Kiełtyka-Dadasiewicz Anna , Głowacka Aleksandra , Ahmed Mohamed A. A.
Published: May 2024

Climate variability is a major challenge concerning food security; therefore, there is a need for pragmatic solutions to improve agricultural production. Henceforth, this study was planned to optimize the planting time of exotic safflowers under the prevailing conditions in Faisalabad, Pakistan. A study was executed by employing a split-plot design with six safflower accessions and five sowing dates ranging from 31 October 2019 to 31 December 2019. The results of the experimental safflower accession PI-198990 produced significant seed yields (2432 kg ha−1, 2772 kg ha−1 and 2366 kg ha−1) when sown on 30 November 2019, 15 December 2019, and 31 December 2019, respectively. On the other hand, on 31 October 2019 and 30 November 2019, sown safflower accessions PI-208677 and PI-250187 were the best performers, respectively. However, a higher achene oil percentage (31.5% and 30.8%) was noted in accessions PI-250187 and PI-314650 when sown on 31 December 2019 and 15 December 2019, respectively. The highest oleic acid content (22.92% and 22.83%) was determined in accession PI-314650 when planted on 30 November 2019 and 15 December 2019, respectively, whereas a higher linoleic acid content was observed on 31 October 2019 and 30 November 2019. Stability analysis showed that safflower accession PI-210834 was the most stable under all sowing environments, followed by PI-314650. Correlation analysis showed that oil percentage showed a negative correlation with phenological traits and growing degree days, and oil yield showed a strong positive relationship with heads, seed yield, biological yield, thousand seed weight, and harvest index.
Spatiotemporal Evaluation of Water Resources in Citarum Watershed during Weak La Nina and Weak El Nino
Authors: Susandi Armi , Darmawan Arief , Sulaiman Albertus , Dewantoro Mouli De Rizka , Wijaya Aristyo Rahadian , Riyadi Agung , Salim Agus , Darmawan Rafif Rahman , Pratama Angga Fauzan
Published: May 2024

This study investigates the dynamics of water resources in the Citarum watershed during periods of weak La Niña, normal, and weak El Niño conditions occurring sequentially. The Citarum watershed serves various purposes, being utilized not only by seven (7) districts and two (2) cities in West Java, Indonesia but also as a source of raw water for drinking in the City of Jakarta. Using a time-series analysis of surface water data, data-driven (machine learning) methods, and statistical analysis methods, spatiotemporal predictions of surface water have been made. The surface water time series data (2017–2021), obtained from in situ instruments, are used to assess water resources, predict groundwater recharge, and analyze seasonal patterns. The results indicate that surface water follows a seasonal pattern, particularly during the monsoon season, corresponding to the groundwater recharge pattern. In upstream areas, water resources exhibit an increasing trend during both weak La Nina and weak El Niño, except for Jatiluhur Dam, where a decline is observed in both seasons. Machine learning predictions suggest that water levels and groundwater recharge tend to decrease in both upstream and downstream areas.
Relationship between Environmental Conditions and Utilisation of Community-Based Mental Health Care: A Comparative Study before and during the COVID-19 Pandemic in Italy
Authors: Prina Eleonora , Tedeschi Federico , Lasalvia Antonio , Salazzari Damiano , Latini Sara , Rabbi Laura , Marando Federica , van Rijn Elaine , Wollgast Jan , Pisoni Enrico , Bessagnet Bertrand , Beauchamp Maxime , Amaddeo Francesco
Published: May 2024

(1) Background: Lower socioeconomic status increases psychiatric service use, exacerbated during the COVID-19 pandemic by environmental stressors like air pollution and limited green spaces. This study aims to assess the influence of sociodemographic and environmental factors on mental health service utilisation. (2) Methods: This retrospective study uses an administrative database focusing on community mental health services in Northeast Italy. Spatial and temporal analyses were used to address space–time dependencies. (3) Results: Findings showed that sociodemographic factors like living in rented apartments and lower education levels predicted higher mental health service use. Environmental factors, such as elevated NO2 levels and, before the pandemic, lower solar radiation and tree cover, correlated with increased service utilisation. COVID-19 reduced most of the pre-existing differences associated with these factors across census blocks with a different composition of sociodemographic and environmental factors. (4) Conclusions: These findings contribute to a better understanding of the impact of the environment on public mental health.
MS785-MS27 Reactive Misfolded/Non-Native Zn-Deficient SOD1 Species Exhibit Cytotoxicity and Adopt Heterozygous Conformations in Motor Neurons
Authors: Tokuda Eiichi , Sakashita Yume , Tokoro Naoya , Date Ayano , Kosuge Yasuhiro , Miyasaka Tomohiro
Published: May 2024

Misfolding of superoxide dismutase-1 (SOD1) is a pathological hallmark of amyotrophic lateral sclerosis (ALS) with SOD1 mutations. The development of antibodies specific for misfolded SOD1 deepens our understanding of how the protein participates in ALS pathogenesis. Since the term “misfolding” refers to various disordered conformers other than the natively folded one, which misfolded species are recognized by specific antibodies should be determined. Here, we molecularly characterized the recognition by MS785-MS27, an antibody cocktail experimentally confirmed to recognize over 100 ALS-linked SOD1 mutants. Indirect ELISA revealed that the antibody cocktail recognized Zn-deficient wild-type and mutated SOD1 species. It also recognized conformation-disordered wild-type and mutated SOD1 species, such as unfolded and oligomeric forms, but had less affinity for the aggregated form. Antibody-reactive SOD1 exhibited cytotoxicity to a motor neuron cell model, which was blocked by Zn treatment with Zn-deficient SOD1. Immunohistochemistry revealed antibody-reactive SOD1 mainly in spinal motor neurons of SOD1G93A mice throughout the disease course, and the distribution after symptomatic stages differed from that of other misfolded SOD1 species. This suggests that misfolded/non-native SOD1 species exist as heterogeneous populations. In conclusion, MS785-MS27 recognizes various conformation-disordered SOD1 species lacking the Zn ion.
The Biological Role of Platelet Derivatives in Regenerative Aesthetics
Authors: Santos Lorena Cristina , Lana Giselle Lobo , Santos Gabriel Silva , Visoni Silvia Beatriz Coutinho , Brigagão Rayssa Junqueira , Santos Napoliane , Sobreiro Rafaela , da Cruz Silva Reis Andreza , Rodrigues Bruno Lima , Ferrari Sabrina , Tambeli Claudia Herrera , Lana José Fábio
Published: May 2024

Bioproducts derived from platelets have been extensively used across various medical fields, with a recent notable surge in their application in dermatology and aesthetic procedures. These products, such as platelet-rich plasma (PRP) and platelet-rich fibrin (PRF), play crucial roles in inducing blood vessel proliferation through growth factors derived from peripheral blood. PRP and PRF, in particular, facilitate fibrin polymerization, creating a robust structure that serves as a reservoir for numerous growth factors. These factors contribute to tissue regeneration by promoting cell proliferation, differentiation, and migration and collagen/elastin production. Aesthetic medicine harnesses these effects for diverse purposes, including hair restoration, scar treatment, striae management, and wound healing. Furthermore, these biological products can act as adjuvants with other treatment modalities, such as laser therapy, radiofrequency, and microneedling. This review synthesizes the existing evidence, offering insights into the applications and benefits of biological products in aesthetic medicine.
Evaluating the Impact of Probiotic Therapy on the Endocannabinoid System, Pain, Sleep and Fatigue: A Randomized, Double-Blind, Placebo-Controlled Trial in Dancers
Authors: Wiącek Jakub , Podgórski Tomasz , Kusy Krzysztof , Łoniewski Igor , Skonieczna-Żydecka Karolina , Karolkiewicz Joanna
Published: May 2024

Emerging research links the endocannabinoid system to gut microbiota, influencing nociception, mood, and immunity, yet the molecular interactions remain unclear. This study focused on the effects of probiotics on ECS markers—cannabinoid receptor type 2 (CB2) and fatty acid amide hydrolase (FAAH)—in dancers, a group selected due to their high exposure to physical and psychological stress. In a double-blind, placebo-controlled trial (ClinicalTrials.gov NCT05567653), 15 dancers were assigned to receive either a 12-week regimen of Lactobacillus helveticus Rosell-52 and Bifidobacterium longum Rosell-17 or a placebo (PLA: n = 10, PRO: n = 5). There were no significant changes in CB2 (probiotic: 0.55 to 0.29 ng/mL; placebo: 0.86 to 0.72 ng/mL) or FAAH levels (probiotic: 5.93 to 6.02 ng/mL; placebo: 6.46 to 6.94 ng/mL; p > 0.05). A trend toward improved sleep quality was observed in the probiotic group, while the placebo group showed a decline (PRO: from 1.4 to 1.0; PLA: from 0.8 to 1.2; p = 0.07841). No other differences were noted in assessed outcomes (pain and fatigue). Probiotic supplementation showed no significant impact on CB2 or FAAH levels, pain, or fatigue but suggested potential benefits for sleep quality, suggesting an area for further research.
Minimal Change Disease: Pathogenetic Insights from Glomerular Proteomics
Authors: Bărar Andrada Alina , Pralea Ioana-Ecaterina , Maslyennikov Yuriy , Munteanu Raluca , Berindan-Neagoe Ioana , Pîrlog Radu , Rusu Ioana , Nuțu Andreea , Rusu Crina Claudia , Moldovan Diana Tania , Potra Alina Ramona , Tirinescu Dacian , Ticala Maria , Elec Florin Ioan , Iuga Cristina Adela , Kacso Ina Maria
Published: May 2024

The mechanism underlying podocyte dysfunction in minimal change disease (MCD) remains unknown. This study aimed to shed light on the potential pathophysiology of MCD using glomerular proteomic analysis. Shotgun proteomics using label-free quantitative mass spectrometry was performed on formalin-fixed, paraffin-embedded (FFPE) renal biopsies from two groups of samples: control (CTR) and MCD. Glomeruli were excised from FFPE renal biopsies using laser capture microdissection (LCM), and a single-pot solid-phase-enhanced sample preparation (SP3) digestion method was used to improve yield and protein identifications. Principal component analysis (PCA) revealed a distinct separation between the CTR and MCD groups. Forty-eight proteins with different abundance between the two groups (p-value ≤ 0.05 and |FC| ≥ 1.5) were identified. These may represent differences in podocyte structure, as well as changes in endothelial or mesangial cells and extracellular matrix, and some were indeed found in several of these structures. However, most differentially expressed proteins were linked to the podocyte cytoskeleton and its dynamics. Some of these proteins are known to be involved in focal adhesion (NID1 and ITGA3) or slit diaphragm signaling (ANXA2, TJP1 and MYO1C), while others are structural components of the actin and microtubule cytoskeleton of podocytes (ACTR3 and NES). This study suggests the potential of mass spectrometry-based shotgun proteomic analysis with LCM glomeruli to yield valuable insights into the pathogenesis of podocytopathies like MCD. The most significantly dysregulated proteins in MCD could be attributable to cytoskeleton dysfunction or may be a compensatory response to cytoskeleton malfunction caused by various triggers.