bluuebunny/crossref_metadata_2025 huggingface dataset:
Insulin Signaling Pathway Mediates FoxO–Pepck Axis Regulation of Glucose Homeostasis in Drosophila suzukii
Authors: Zang Shuting , Wang Ruijuan , Liu Yan , Zhao Shan , Su Long , Dai Xiaoyan , Chen Hao , Yin Zhenjuan , Zheng Li , Liu Qingxin , Zhai Yifan
Published: September 2024

The agricultural pest Drosophila suzukii exhibits a strong preference for feeding on fresh fruits, demonstrating high adaptability to sugary environments. Meanwhile, high sugar levels stimulate insulin secretion, thereby regulating the steady state of sugar metabolism. Understanding the mechanisms related to sugar metabolism in D. suzukii is crucial due to its adaptation to these specific environmental conditions. The insulin signaling pathway is an evolutionarily conserved phosphorylation cascade with significant roles in development and metabolism. We observed that the activation of the insulin signaling pathway inhibited FoxO activity and downregulated the expression of Pepck, thereby activating glycolysis and reducing glucose levels. By contrast, inhibiting insulin signaling increased the FoxO activity and upregulated the expression of Pepck, which activated gluconeogenesis and led to increased glucose levels. Our findings demonstrated the crucial role of the insulin signaling pathway in mediating glucose metabolism through the FoxO–Pepck axis, which supports the ecological adaptation of D. suzukii to high-sugar niches, thereby providing insights into its metabolic control and suggesting potential strategies for pest management. Elucidating these molecular processes is important for understanding metabolic regulation and ecological specialization in D. suzukii.
Circadian Rhythm Genes and Their Association with Sleep and Sleep Restriction
Authors: Sochal Marcin , Ditmer Marta , Tarasiuk-Zawadzka Aleksandra , Binienda Agata , Turkiewicz Szymon , Wysokiński Adam , Karuga Filip Franciszek , Białasiewicz Piotr , Fichna Jakub , Gabryelska Agata
Published: September 2024

Deprivation of sleep (DS) and its effects on circadian rhythm gene expression are not well understood despite their influence on various physiological and psychological processes. This study aimed to elucidate the changes in the expression of circadian rhythm genes following a night of sleep and DS. Their correlation with sleep architecture and physical activity was also examined. The study included 81 participants who underwent polysomnography (PSG) and DS with actigraphy. Blood samples were collected after PSG and DS. Expression levels of brain and muscle ARNT-like 1 (BMAL1), circadian locomotor output cycles kaput (CLOCK), neuronal PAS domain protein 2 (NPAS2), period 1 (PER1), cryptochrome 1 (CRY1) and nuclear receptor subfamily 1 group D member 1 (NR1D1) were analyzed using qRT-PCR. DS decreased the expression of CLOCK and BMAL1 while increasing PER1. PER1 expression correlated positively with total sleep time and non-rapid-eye-movement (NREM) sleep duration and negatively with sleep latency, alpha, beta and delta waves in the O1A2 lead. Physical activity during DS showed positive correlations with CLOCK, BMAL1, and CRY1. The findings highlight the role of PER1 in modulating sleep patterns, suggesting potential targets for managing sleep-related disorders. Further research is essential to deepen the understanding of these relationships and their implications.
Physico-Chemical Characterization and Initial Evaluation of Carboxymethyl Chitosan–Hyaluronan Hydrocolloid Systems with Insulin Intended for Intranasal Administration
Authors: Popescu Roxana , Dinu-Pîrvu Cristina-Elena , Ghica Mihaela Violeta , Anuța Valentina , Popa Lăcrămioara
Published: September 2024

The nasal route of administration can bypass the blood–brain barrier in order to obtain a higher concentration in the brain, thus offering a feasible alternative route of administration for diseases associated with the central nervous system. The advantages of the intranasal administration and the potential favorable therapeutic effects of intranasally administered insulin led to the formulation of carboxymethyl chitosan (CMC) and sodium hyaluronate (NaHA) hydrocolloidal systems with insulin for nasal administration, targeting nose-to-brain delivery and the initial assessment of these systems. The influence of the formulation variables on the response parameters defined as surface properties, rheology, and in vitro release of insulin were analyzed using experimental design and statistical programs (Modde and Minitab software). The systems recorded good wetting and adhesion capacity, allowing the spread of the hydrocolloidal systems on the nasal mucosa. The samples had a pseudoplastic flow and the rapid release of the insulin was according to our objective. According to the physico-chemical characterization and preliminary assessment, these formulations are appropriate for administration on the nasal mucosa, but further studies are necessary to demonstrate the beneficial therapeutic actions and the safety of using intranasal insulin.
Quantification of Letrozole, Palbociclib, Ribociclib, Abemaciclib, and Metabolites in Volumetric Dried Blood Spots: Development and Validation of an LC-MS/MS Method for Therapeutic Drug Monitoring
Authors: Cecchin Eleonora , Orleni Marco , Gagno Sara , Montico Marcella , Peruzzi Elena , Roncato Rossana , Gerratana Lorenzo , Corsetti Serena , Puglisi Fabio , Toffoli Giuseppe , Cecchin Erika , Posocco Bianca
Published: September 2024

Therapeutic drug monitoring (TDM) may be beneficial for cyclin-dependent kinase 4/6 inhibitors (CDK4/6is), such as palbociclib, ribociclib, and abemaciclib, due to established exposure–toxicity relationships and the potential for monitoring treatment adherence. Developing a method for quantifying CDK4/6is, abemaciclib metabolites (M2, M20), and letrozole in dried blood spots (DBS) could be useful to enhance the feasibility of TDM. Thus, an optimized LC-MS/MS method was developed using the HemaXis DB10 device for volumetric (10 µL) DBS collection. Chromatographic separation was achieved using a reversed-phase XBridge BEH C18 column. Detection was performed with a triple quadrupole mass spectrometer, utilizing ESI source switching between negative and positive ionization modes and multiple reaction monitoring acquisition. Analytical validation followed FDA, EMA, and IATDMCT guidelines, demonstrating high selectivity, adequate sensitivity (LLOQ S/N ≥ 30), and linearity (r ≥ 0.997). Accuracy and precision met acceptance criteria (between-run: accuracy 95–106%, CV ≤ 10.6%). Haematocrit independence was confirmed (22–55%),with high recovery rates (81–93%) and minimal matrix effects (ME 0.9–1.1%). The stability of analytes under home-sampling conditions was also verified. Clinical validation supports DBS-based TDM as feasible, with conversion models developed for estimating plasma concentrations (the reference for TDM target values) of letrozole, abemaciclib, and its metabolites. Preliminary data for palbociclib and ribociclib are also presented.
Graph-Based Semi-Supervised Learning with Bipartite Graph for Large-Scale Data and Prediction of Unseen Data
Authors: Alemi Mohammad , Bosaghzadeh Alireza , Dornaika Fadi
Published: September 2024

Recently, considerable attention has been directed toward graph-based semi-supervised learning (GSSL) as an effective approach for data labeling. Despite the progress achieved by current methodologies, several limitations persist. Firstly, many studies treat all samples equally in terms of weight and influence, disregarding the potential increased importance of samples near decision boundaries. Secondly, the detection of outlier-labeled data is crucial, as it can significantly impact model performance. Thirdly, existing models often struggle with predicting labels for unseen test data, restricting their utility in practical applications. Lastly, most graph-based algorithms rely on affinity matrices that capture pairwise similarities across all data points, thus limiting their scalability to large-scale databases. In this paper, we propose a novel GSSL algorithm tailored for large-scale databases, leveraging anchor points to mitigate the challenges posed by large affinity matrices. Additionally, our method enhances the influence of nodes near decision boundaries by assigning different weights based on their importance and using a mapping function from feature space to label space. Leveraging this mapping function enables direct label prediction for test samples without requiring iterative learning processes. Experimental evaluations on two extensive datasets (Norb and Covtype) demonstrate that our approach is scalable and outperforms existing GSSL methods in terms of performance metrics.
Energy Analysis of Standardized Shipping Containers for Housing
Authors: Fariña Elena Arce , Panait Mirela , Lago-Cabo José María , Fernández-González Raquel
Published: September 2024

Shipping containers that remain in ports after exporting or importing products cause an environmental and logistical problem. Transporting them to the port of origin is costly; therefore, some of them are stored in the regions of destination. Recycling or reusing them in an efficient and sustainable way represents a clean alternative. The purpose of this article is to analyze the feasibility and impact of implementing different insulating configurations on the energy demands required by a house based on a construction with standardized shipping containers. More specifically, it assesses the impact of the different orientations in which the dwelling can be arranged, depending on the location and its meteorological data. To this aim, a construction model will be developed in which first, the geometrical parameters are defined, and second, the energy characteristics are identified. The results show that, in Southwest Europe, the western orientation generates a saving of 10% of the energy demand compared to the less favourable orientation, which is the southern one.
The Long-Term Results of Covered Endovascular Aortic Bifurcation Repair in Complex Aortoiliac Disease: A Two-Year Follow-Up
Authors: Dikmen Nur , Ozcinar Evren , Akça Fatma , Sen Emre , Karacuha Ali Fuat , Kayan Ahmet , Yazicioglu Levent
Published: September 2024

Background: We aimed to investigate the two-year outcomes of covered endovascular reconstruction (CERAB) of the aortic bifurcation in patients with complex aortoiliac occlusive dis ease. Methods: This study was prospectively initiated, with data retrospectively collected from 40 patients categorized as TASC II B, C, and D based on computed tomography angiography (CTA) findings. All patients underwent the CERAB procedure. We assessed the procedural outcomes, including clinical and symptomatic improvements, as well as patency rates over a two-year follow-up period. Results: A total of 40 patients (33 males and 7 females) with aorto-occlusive disease were treated using the CERAB procedure and included in this observational study. The technical success rate was 100% across all procedures. At 36 months, the overall primary patency, assisted primary patency, and secondary patency rates were 85%, 90%, and 92.5%, respectively. Conclusions: The two-year results of this study suggest that CERAB offers patency rates comparable to those reported in other studies for complex aorto-occlusive bifurcation diseases. The procedure showed favorable patency rates, particularly for more advanced TASC II B, C, and D lesions.
Minimal Invasive Pre-Op CT-Guided Gold-Fiducials in Local Anesthesia for Easy Level Localization in Thoracic Spine Surgery
Authors: Keil Fee , Hagemes Frank , Setzer Matthias , Behmanesh Bedjan , Marquardt Gerhard , Hattingen Elke , Prinz Vincent , Czabanka Marcus , Bruder Markus
Published: September 2024

Background: The accurate identification of intraoperative levels is of paramount importance in spinal surgery, particularly in cases of obesity or anatomical anomalies affecting the thoracic spine. The aim of this work was to clarify whether the preoperative percutaneous placement of fiducial markers under local anesthesia only, with minimal discomfort to the patient, can be performed safely and efficiently. Methods: Patients treated at our institution between June 2019 and June 2020 for thoracic intraspinal lesions with preoperative percutaneous gold fiducial placement were analyzed. A total of 10 patients underwent CT-guided gold fiducial placement 2–48 h prior to surgery on an outpatient or inpatient basis. Patient characteristics, CT intervention time, and perioperative complications were recorded. Results: In all cases, the gold markers were placed under local anesthesia alone and were easily visualized intraoperatively with fluoroscopy. There was no preoperative dislocation or malposition. The procedure was performed without X-ray exposure to the neuroradiology interventionalist. The average CT intervention time from the planning scout to the final control time was 14.3 min. The percentage of anatomical norm variants in our observation group was high, as 2 of the 10 patients had lumbarization of the first sacral vertebra, resulting in a six-link lumbar spine. Conclusions: Preoperative CT-guided transcutaneous submuscular placement of gold markers under local anesthesia is a practical and safe method for rapid and accurate intraoperative level determination in thoracic spine surgery in a time-saving minimally invasive manner. The virtually painless procedure can be performed either preoperatively on an outpatient basis or as an inpatient procedure.
Calcification Formation for Development, Defense, and Repair of the Human Body?
Authors: de Jong Pim A. , Bos Daniel , Mali W. P. Th. M.
Published: September 2024

Calcium deposits commonly occur in the human body in any type of tissue through an actively regulated process [...]
Augmented Reality in Extratemporal Lobe Epilepsy Surgery
Authors: Grote Alexander , Neumann Franziska , Menzler Katja , Carl Barbara , Nimsky Christopher , Bopp Miriam H. A.
Published: September 2024

Background: Epilepsy surgery for extratemporal lobe epilepsy (ETLE) is challenging, particularly when MRI findings are non-lesional and seizure patterns are complex. Invasive diagnostic techniques are crucial for accurately identifying the epileptogenic zone and its relationship with surrounding functional tissue. Microscope-based augmented reality (AR) support, combined with navigation, may enhance intraoperative orientation, particularly in cases involving subtle or indistinct lesions, thereby improving patient outcomes and safety (e.g., seizure freedom and preservation of neuronal integrity). Therefore, this study was conducted to prove the clinical advantages of microscope-based AR support in ETLE surgery. Methods: We retrospectively analyzed data from ten patients with pharmacoresistant ETLE who underwent invasive diagnostics with depth and/or subdural grid electrodes, followed by resective surgery. AR support was provided via the head-up displays of the operative microscope, with navigation based on automatic intraoperative computed tomography (iCT)-based registration. The surgical plan included the suspected epileptogenic lesion, electrode positions, and relevant surrounding functional structures, all of which were visualized intraoperatively. Results: Six patients reported complete seizure freedom following surgery (ILAE 1), one patient was seizure-free at the 2-year follow-up, and one patient experienced only auras (ILAE 2). Two patients developed transient neurological deficits that resolved shortly after surgery. Conclusions: Microscope-based AR support enhanced intraoperative orientation in all cases, contributing to improved patient outcomes and safety. It was highly valued by experienced surgeons and as a training tool for less experienced practitioners.
Characterization of Circulating Protein Profiles in Individuals with Prader–Willi Syndrome and Individuals with Non-Syndromic Obesity
Authors: Pascut Devis , Giraudi Pablo José , Banfi Cristina , Ghilardi Stefania , Tiribelli Claudio , Bondesan Adele , Caroli Diana , Grugni Graziano , Sartorio Alessandro
Published: September 2024

Background: Prader–Willi syndrome (PWS) is a rare genetic disorder characterized by distinctive physical, cognitive, and behavioral manifestations, coupled with profound alterations in appetite regulation, leading to severe obesity and metabolic dysregulation. These clinical features arise from disruptions in neurodevelopment and neuroendocrine regulation, yet the molecular intricacies of PWS remain incompletely understood. Methods: This study aimed to comprehensively profile circulating neuromodulatory factors in the serum of 53 subjects with PWS and 34 patients with non-syndromic obesity, utilizing a proximity extension assay with the Olink Target 96 neuro-exploratory and neurology panels. The ANOVA p-values were adjusted for multiple testing using the Benjamani–Hochberg method. Protein–protein interaction networks were generated in STRING V.12. Corrplots were calculated with R4.2.2 by using the Hmisc, Performance Analytics, and Corrplot packages Results: Our investigation explored the potential genetic underpinnings of the circulating protein signature observed in PWS, revealing intricate connections between genes in the PWS critical region and the identified circulating proteins associated with impaired oxytocin, NAD metabolism, and sex-related neuromuscular impairment involving, CD38, KYNU, NPM1, NMNAT1, WFIKKN1, and GDF-8/MSTN. The downregulation of CD38 in individuals with PWS (p < 0.01) indicates dysregulation of oxytocin release, implicating pathways associated with NAD metabolism in which KYNU and NMNAT1 are involved and significantly downregulated in PWS (p < 0.01 and p < 0.05, respectively). Sex-related differences in the circulatory levels of WFIKKN1 and GDF-8/MSTN (p < 0.05) were also observed. Conclusions: This study highlights potential circulating protein biomarkers associated with impaired oxytocin, NAD metabolism, and sex-related neuromuscular impairment in PWS individuals with potential clinical implications.
Unraveling of Molecular Mechanisms of Cognitive Frailty in Chronic Kidney Disease: How Exercise Makes a Difference
Authors: Michou Vasiliki , Tsamos Georgios , Vasdeki Dimitra , Deligiannis Asterios , Kouidi Evangelia
Published: September 2024

As our population ages, the medical challenges it faces become increasingly acute, with chronic kidney disease (CKD) becoming more prevalent among older adults. Frailty is alarmingly more common in CKD patients than in the general populace, putting the elderly at high risk of both physical and cognitive decline. CKD not only accelerates physical deterioration, but also heightens vascular dysfunction, calcification, arterial rigidity, systemic inflammation, oxidative stress, and cognitive impairment. Cognitive frailty, a distinct syndrome marked by cognitive deficits caused by physiological causes (excluding Alzheimer’s and other dementias), is a critical concern. Although cognitive impairment has been well-studied, the molecular mechanisms driving cognitive frailty remain largely uncharted. Comprehensive interventions, including cutting-edge pharmaceuticals and lifestyle changes, are pivotal and effective, especially in the early stages of CKD. Recent research suggests that systematic exercise could counteract cognitive decline by improving brain blood flow, boosting neuroplasticity through the brain-derived neurotrophic factor (BDNF), and by triggering the release of neurotrophic factors such as insulin-like growth factor (IGF-1). This review delves into the molecular pathways of cognitive frailty in CKD, identifies key risk factors, and highlights therapeutic approaches, particularly the potent role of exercise in enhancing cognitive health.
Prognostic Evaluation of Disorders of Consciousness by Using Resting-State fMRI: A Systematic Review
Authors: Le Cause Maria , Bonanno Lilla , Alagna Antonella , Bonanno Carmen , De Caro Jolanda , Logiudice Anna Lisa , Pollicino Patrizia , Corallo Francesco , De Salvo Simona , Rifici Carmela , Quartarone Angelo , Marino Silvia
Published: September 2024

Background: This review focuses on the prognostic role of resting-state functional magnetic resonance imaging (fMRI) in disorders of consciousness (DOCs). Several studies were conducted to determine the diagnostic accuracy in DOC patients to identify prognostic markers and to understand the neural correlates of consciousness. A correct diagnosis of consciousness in unresponsive or minimally responsive patients is important for prognostic and therapeutic management. Functional connectivity is considered as an important tool for the formulation of cerebral networks; it takes into account the primary sensorimotor, language, visual and central executive areas, where fMRI studies show damage in brain connectivity in the areas of frontoparietal networks in DOC patients. Methods: The integration of neuroimaging or neurophysiological methods could improve our knowledge of the neural correlates of clinical response after an acquired brain injury. The use of MRI is widely reported in the literature in different neurological diseases. In particular, fMRI is the most widely used brain-imaging technique to investigate the neural mechanisms underlying cognition and motor function. We carried out a detailed literature search following the relevant guidelines (PRISMA), where we collected data and results on patients with disorders of consciousness from the studies performed. Results: In this review, 12 studies were selected, which showed the importance of the prognostic role of fMRI for DOCs. Conclusions: Currently there are still few studies on this topic. Future studies using fMRI are to be considered an added value for the prognosis and management of DOCs.
The Redefinition and Volumization of the Lip Area with Hyaluronic Acid: A Case Series
Authors: Ruiz Nazaret , Lopez Roberto Miranda , Marques Rubén , Fontenete Silvia
Published: September 2024

Background: The increasing popularity of non-surgical cosmetic enhancements for the lower face and perioral area, particularly through hyaluronic acid (HA) fillers, reflects the growing desire for improved lip volume and definition. This study showcases the effects of a specific HA filler on lip fullness, shape, and overall perioral rejuvenation. Methods: We conducted a retrospective single-site observational analysis of adult female patients treated with Genefill Soft Fill HA injections in the lips and perioral areas. Both patient and physician satisfaction were evaluated using the Likert scale and Global Aesthetic Improvement Scale (GAIS), respectively. The outcomes for natural appearance, volume, and durability were assessed using a five-point scale. The patients were followed up with for up to six months to monitor any adverse events. Results: The cohort included thirteen female patients with an average age of 55.3 ± 8.3 years. Approximately 1.2 ± 0.4 mL of filler was used per patient. The results indicate high satisfaction, with scores above 4 for naturalness, volume, and durability. Over 92% of patients reported a significant improvement in appearance. No moderate or severe adverse events were reported. Conclusions: Genefill Soft Fill HA filler is both effective and safe for enhancing lip esthetics, with high satisfaction rates among recipients and no significant adverse events observed.
Memory-Guided Saccades and Non-Motor Symptoms Improve after Botulinum Toxin Therapy in Cervical Dystonia
Authors: Gilman Kuric Tihana , Popovic Zvonimir , Matosa Sara , Sadikov Aleksander , Groznik Vida , Georgiev Dejan , Gerbasi Alessia , Kragujevic Jagoda , Mirosevic Zubonja Tea , Krivdic Dupan Zdravka , Guljas Silva , Kuric Igor , Juric Stjepan , Palic Kramaric Ruzica , Tomic Svetlana
Published: September 2024

Background/Objectives: Cervical dystonia (CD) is a condition characterized by involuntary activity of cervical muscles, which is often accompanied by various non-motor symptoms. Recent studies indicate impaired saccadic eye movements in CD. Local administration of botulinum toxin type A (BoNT/A), which causes temporary paralysis of the injected muscle, is the first-line treatment of focal dystonia, including CD. To our knowledge, concurrent observation of the effect of BoNT/A on smooth eye movements, voluntary saccades, memory-guided saccades, and antisaccades in CD has not yet been explored. The aim of this study was to assess the effect of BoNT/A on eye movements and non-motor symptoms in patients with CD, which, when altered, could imply a central effect of BoNT/A. Methods: Thirty patients with CD performed smooth pursuit, prosaccadic expression, memory-guided saccades, and antisaccade tasks; eye movements were recorded by an eye tracker. Motor and non-motor symptoms, including depression, anxiety, pain, disability, and cognitive changes prior to and after BoNT/A administration, were also evaluated. Results: The number of correct onward counts (p < 0.001), overall correct memory-guided saccades count (p = 0.005), motor symptoms (p = 0.001), and non-motor symptoms, i.e., anxiety (p = 0.04), depression (p = 0.02), and cognition (p < 0.001) markedly improved after BoNT/A administration. Conclusions: Memory-guided saccades, depression, and anxiety improve after BoNT/A in CD.
Imaging of Osteosarcoma: Presenting Findings, Metastatic Patterns, and Features Related to Prognosis
Authors: Crombé Amandine , Simonetti Mario , Longhi Alessandra , Hauger Olivier , Fadli David , Spinnato Paolo
Published: September 2024

Background: Osteosarcomas are rare malignancies (<1% of all cancers) that produce an osteoid matrix. Osteosarcomas are the second most frequent type of primary bone tumor after multiple myeloma and the most prevalent primary bone tumor in children. The spectrum of imaging findings of these malignancies varies significantly, reflecting different histological subtypes. For instance, conventional osteosarcoma typically presents with a mixed radiological pattern (lytic and bone mineralization) or with a completely eburneous one; aggressive periosteal reactions such as sunburst, Codman triangle, and soft-tissue components are frequently displayed. On the other hand, telangiectatic osteosarcoma usually presents as a purely lytic lesion with multiple fluid–fluid levels on MRI fluid-sensitive sequences. Other typical and atypical radiological patterns of presentation in other subtypes of osteosarcomas are described in this review. In addition to the characteristics associated with osteosarcoma subtyping, this review article also focuses on imaging features that have been associated with patient outcomes, namely response to chemotherapy and event-free and overall survivals. This includes simple semantic radiological features (such as tumor dimensions, anatomical location with difficulty of radical surgery, occurrence of pathological fractures, and presence of distant metastases), but also quantitative imaging parameters from diffusion-weighted imaging, dynamic contrast-enhanced MRI, and 18F-FDG positron emission tomography and radiomics approaches. Other particular features are described in the text. Overall, this comprehensive literature review aims to be a practical tool for oncologists, pathologists, surgeons, and radiologists involved in these patients’ care.
Transforming Nanomaterial Synthesis through Advanced Microfluidic Approaches: A Review on Accessing Unrestricted Possibilities
Authors: Roy Sanjib , Kumar Ramesh , Acooli Argha , Roy Snehagni , Chatterjee Abhrajit , Chattaraj Sujoy , Nayak Jayato , Jeon Byong-Hun , Basu Aradhana , Banerjee Shirsendu , Chakrabortty Sankha , Tripathy Suraj K.
Published: September 2024

The inception of microfluidic devices marks a confluence of diverse scientific domains, including physics, biology, chemistry, and fluid mechanics. These multidisciplinary roots have catalyzed the evolution of microfluidic devices, which serve as versatile platforms for various chemical and biological processes. Notably, microfluidic devices have garnered attention as efficient reactors, offering distinct benefits such as minimized spatial requirements for reactions, reduced equipment costs, and accelerated residence times. These advantages, among others, have ignited a compelling interest in harnessing microfluidic technology for the conception, refinement, and production of various nanomaterials and nanocomposites, pivotal within both industrial and medicinal sectors. This comprehensive exposition delves into multifaceted aspects of nanomaterial synthesis, underscoring the transformative role of microfluidic methodologies as a departure from conventional techniques. The discourse navigates through intricate considerations surrounding the preparation of nanomaterials, elucidating how the microfluidic paradigm has emerged as a promising alternative. This paper serves as an illuminating exploration of the juncture between microfluidic innovation and nanomaterial synthesis. It traverses the transformative potential of microfluidics in revolutionizing traditional approaches, heralding a new era of precision engineering for advanced materials with applications spanning industrial to medicinal domains.
Functional Scaffolds for Bone Tissue Regeneration: A Comprehensive Review of Materials, Methods, and Future Directions
Authors: Todd Emily Ann , Mirsky Nicholas A. , Silva Bruno Luís Graciliano , Shinde Ankita Raja , Arakelians Aris R. L. , Nayak Vasudev Vivekanand , Marcantonio Rosemary Adriana Chiérici , Gupta Nikhil , Witek Lukasz , Coelho Paulo G.
Published: September 2024

Bone tissue regeneration is a rapidly evolving field aimed at the development of biocompatible materials and devices, such as scaffolds, to treat diseased and damaged osseous tissue. Functional scaffolds maintain structural integrity and provide mechanical support at the defect site during the healing process, while simultaneously enabling or improving regeneration through amplified cellular cues between the scaffold and native tissues. Ample research on functionalization has been conducted to improve scaffold–host tissue interaction, including fabrication techniques, biomaterial selection, scaffold surface modifications, integration of bioactive molecular additives, and post-processing modifications. Each of these methods plays a crucial role in enabling scaffolds to not only support but actively participate in the healing and regeneration process in bone and joint surgery. This review provides a state-of-the-art, comprehensive overview of the functionalization of scaffold-based strategies used in tissue engineering, specifically for bone regeneration. Critical issues and obstacles are highlighted, applications and advances are described, and future directions are identified.
Customizable Lyophilized Agent for Radiotherapy Imaging and TherapY (CLARITY)
Authors: Moreau Michele , China Debarghya , Sy Gnagna , Ding Kai , Ngwa Wilfred
Published: September 2024

Smart radiotherapy biomaterials (SRBs) include seed and liquid biomaterials designed to be employed as fiducial markers during radiotherapy while also delivering therapeutic drug payloads to enhance treatment outcomes. In this study, we investigate a novel Customizable Lyophilized Agent for Radiotherapy Imaging and TherapY (CLARITY) biomaterial, which can be loaded with immunoadjuvants (anti-CD40 monoclonal antibody or Caflanone (FBL-03G)) at the point of care. The CLARITY biomaterial was investigated in an animal model of pancreatic cancer using C57BL6 mice. Mice were imaged before and at different points of time post-treatment to evaluate the potential of CLARITY biomaterial to provide imaging contrast similar to fiducials. This study also used cadavers to assess CLARITY’s potential to provide imaging contrast in humans. Results showed imaging contrast from computed tomography (CT) and magnetic resonance imaging (MRI) modalities for up to 30 days post-treatment, demonstrating potential for use as fiducials. A significant increase in survival (***, p = 0.0006) was observed for mice treated with CLARITY biomaterial loaded with immunoadjuvant for up to 10 weeks post-treatment compared to those without treatment. These initial results demonstrate the potential of CLARITY biomaterial to serve as a smart multifunctional radiotherapy biomaterial and provide the impetus for further development and optimization as a point-of-care technology for combination radiotherapy and immunotherapy.
Decomposing the True Score Variance in Rated Responses to Divergent Thinking-Tasks for Assessing Creativity: A Multitrait–Multimethod Analysis
Authors: Jendryczko David
Published: September 2024

It is shown how the Correlated Traits Correlated Methods Minus One (CTC(M − 1)) Multitrait-Multimethod model for cross-classified data can be modified and applied to divergent thinking (DT)-task responses scored for miscellaneous aspects of creative quality by several raters. In contrast to previous Confirmatory Factor Analysis approaches to analyzing DT-tasks, this model explicitly takes the cross-classified data structure resulting from the employment of raters into account and decomposes the true score variance into target-specific, DT-task object-specific, rater-specific, and rater–target interaction-specific components. This enables the computation of meaningful measurement error-free relative variance-parameters such as trait-consistency, object–method specificity, rater specificity, rater–target interaction specificity, and model-implied intra-class correlations. In the empirical application with alternate uses tasks as DT-measures, the model is estimated using Bayesian statistics. The results are compared to the results yielded with a simplified version of the model, once estimated with Bayesian statistics and once estimated with the maximum likelihood method. The results show high trait-correlations and low consistency across DT-measures which indicates more heterogeneity across the DT-measurement instruments than across different creativity aspects. Substantive deliberations and further modifications, extensions, useful applications, and limitations of the model are discussed.
Output Feedback Adaptive Optimal Control of Multiple Unmanned Marine Vehicles with Unknown External Disturbance
Authors: Yuan Liang-En , Xiao Yang , Li Tieshan , Zhou Dalin
Published: September 2024

This paper presents an optimal output-feedback tracking control problem for multiple unmanned marine vehicles (UMVs) to track a desired trajectory. To guarantee the control objective in an optimal manner, adaptive dynamic programming (ADP) with optimal compensation terms is adopted. A neural velocity observer is designed based on a neural network (NN) to estimate the unmeasured system states and the unknown system dynamics. Furthermore, a disturbance observer (DO) is proposed to go against the effect of the unknown external disturbance of the sea environment. It is proved that the proposed controller can guarantee that all signals in the closed-loop system are bounded. Simulation results are given to demonstrate the effectiveness of the proposed control algorithm.
A Review of the Hydroelastic Theoretical Models of Floating Porous Nets and Floaters for Offshore Aquaculture
Authors: Mohapatra Sarat Chandra , Guedes Soares C.
Published: September 2024

The present review focuses on the theoretical model developments made in floating flexible net fish cages and the floating bodies application to offshore aquaculture. A brief discussion of the essential mathematical equations related to various theoretical models of flexible net cages in the frequency domain is presented. The single and array of floating or submerged flexible net cages connected with or without mooring lines are discussed. Further, as the combined effect of the hydroelastic behaviour of floaters and the flexible behaviour of fish cages are necessary to assess their efficiency and survivability from structural damages, the issues and the knowledge gap between the recent and future models are also discussed. In conclusion, the practical suggestions concerning advancements in future research and directions within floating flexible net cages and the hydroelastic response of elastic floaters are highlighted.
Platform First-Party Product Entry and Pricing Strategy under Cost Differences and Capacity Constraints
Authors: Chen Haijun , Xu Qi
Published: September 2024

In the rapidly evolving platform economy, the competition between platform-owned products and third-party offerings is intensifying. This study examines the entry and pricing strategies of dominant e-commerce platforms such as Amazon and JD Mall which sell both platform-owned and third-party products. We use a complete information game model to analyze the strategic interactions between these platforms and third-party sellers, focusing on cost discrepancies and limited entry capabilities, areas previously underexplored. Our key findings include the following: (1) Platforms with dominant power can restrict third-party product pricing. (2) Increased consumer influence by the platform can reduce competition between platform-owned and third-party products. (3) Platforms prioritize high-value-product markets when entry capabilities are limited. (4) Commission-based revenue models are generally more efficient than entry fees. (5) Regulatory bans on hybrid models do not necessarily enhance social welfare; differentiated taxation on various revenue sources may be more effective. This study contributes by developing a comprehensive game-theoretic model to simulate strategic interactions, analyze pricing competition and entry strategies under cost asymmetry and capacity constraints, and provide theoretical guidance for regulatory policies.
Empirical Research of Cold-Chain Logistics Service Quality in Fresh Product E-Commerce
Authors: Wang Ling , Tang Yuk-Ming , Chau Ka-Yin , Zheng Xiaoxuan
Published: September 2024

Logistics service quality (LSQ) plays a vital role in providing excellent customer experience, particularly in e-commerce. Using mobile devices for food and fresh product orders is very common, but delivering these products is very challenging. In this study, we aim to evaluate the factors influencing logistics service quality (LSQ) in the context of fresh product e-commerce. The relevant literature was reviewed and a preliminary survey among field experts was conducted to establish the proposed LSQ scale. A qualitative study was carried out on fresh product e-commerce customers. A survey involving 222 participants was analyzed, and an LSQ evaluation scale was formed and evaluated scientifically and empirically. Research results showed that reliability, convenience, freshness, and personnel contact quality are the four key dimensions of the LSQ scale in the e-commerce platform for fresh and perishable items. The results of the study can help the managers of e-commerce companies to understand the LSQ criteria that determine customer satisfaction and consequently make the appropriate LSQ improvements.
Mapping an Indicator Species of Sea-Level Rise along the Forest–Marsh Ecotone
Authors: Norlin Bryanna , Scholl Andrew E. , Case Andrea L. , Assal Timothy J.
Published: September 2024

Atlantic White Cedar (Chamaecyparis thyoides) (AWC) anchors a globally threatened ecosystem that is being impacted by climate change, as these trees are vulnerable to hurricane events, sea-level rises, and increasing salinity at the forest–marsh ecotone. In this study, we determined the current amount and distribution of AWC in an area that is experiencing sea-level rises that are higher than the global average rate. We used a combination of a field investigation and aerial photo interpretation to identify known locations of AWC, then integrated Sentinel-1 and 2A satellite data with abiotic variables into a species distribution model. We developed a spectral signature of AWC to aid in our understanding of phenology differences from nearby species groups. The selected model had an out-of-bag error of 7.2%, and 8 of the 11 variables retained in the final model were derived from remotely sensed data, highlighting the importance of including temporal data to exploit divergent phenology. Model predictions were strong in live AWC stands and, accurately, did not predict live AWC in stands that experienced high levels of mortality after Hurricane Sandy. The model presented in this study provides high utility for AWC management and tracking mortality dynamics within stands after disturbances such as hurricanes.
Effects of Patchiness on Soil Properties and Degradation of Alpine Meadow on the Qinghai–Tibetan Plateau
Authors: Zhang Wei , Yi Shuhua , Qin Yu , Zhang Jinglin
Published: September 2024

Patchiness is known to affect soil water and soil temperature, and may trigger or accelerate alpine meadow degradation. However, there is a lack of direct evidence concerning the role of the size and type of patches on soil conditions. Here, we estimated the effects of typical patch types on the critical factors of soil water and soil temperature through an in situ survey and then analyzed the potential impacts of patchiness on alpine meadow degradation. The results showed that (1) the soil water storage (SWS) of typical patch types was remarkably different, and vegetation patches had higher SWS than bare patches; (2) with abundant precipitation, the isolated vegetation patch (IV) had higher SWS than the original vegetation patch (OV), accompanied by a decrease in the SWS of the surrounding medium bare patch (MP); (3) patchiness significantly altered the surface soil temperature, with the IV having the highest soil temperature (ST), followed by the OV and bare patches; and (4) the maximum mean value of evapotranspiration was observed in IV (3.85 mm/day), about 12.78% and 46.66% higher than in the bare patches and OV. Our findings indicated that patchiness intensified the heterogeneity of soil water and soil temperature. The IV could absorb soil water from surrounding MP for excessive evapotranspiration through constant high ST, potentially inducing or aggravating degradation. Increasing the connectivity of IV and preventing the transition of OV to IV is important for the stability and restoration of alpine meadow.
Palmitoylethanolamide (PEA) for Prevention of Gastroesophageal Inflammation: Insights from In Vitro Models
Authors: Spigarelli Renato , Calabrese Carlo , Spisni Enzo , Vinciguerra Sara , Saracino Ilaria Maria , Dussias Nikolas Kostantine , Filippone Eleonora , Valerii Maria Chiara
Published: September 2024

Gastroesophageal reflux disease (GERD) is a digestive disorder that can lead to chronic mucosal damage, causing esophagitis, Barrett’s esophagus and esophageal cancer. GERD currently affects about 13% of the world’s population and represent a major public health concern due to the increasing prevalence and incidence. The aim of this study was to explore complementary strategies for GERD management based the natural compound palmitoylethanolamide (PEA), alone or associated with plant extracts with demonstrated anti-GERD activity (Zingiber officinale, Musa × paradisiaca, Opuntia ficus-indica and Olea europaea). For this purpose, two in vitro models based on the esophageal mucosa CP-B cell line were chosen. The first one was based on the exposure of esophageal cells to HCl, while the second one was based on lipopolysaccharide (LPS) treatment to cause a strong inflammatory cell response. Inflammation induced was assessed using a Luminex® assay, measuring the secretion of IL-1β, IL-6, IL-10, IL-8 and TNF-α. Results obtained demonstrate that PEA strongly decreased the inflammatory response elicited by HCl exposure. Moreover, the effect of PEA was enhanced by the presence of natural extracts of Zingiber officinale, Musa × paradisiaca, Opuntia ficus-indica and Olea europaea. PEA should be considered as an anti-GERD natural compound of interest.
Mechanisms of Resistance to Rituximab Used for the Treatment of Autoimmune Blistering Diseases
Authors: Popa Liliana Gabriela , Dumitras Ioana , Giurcaneanu Calin , Berghi Ovidiu , Radaschin Diana Sabina , Vivisenco Cristina Iolanda , Popescu Marius Nicolae , Beiu Cristina
Published: September 2024

Autoimmune blistering diseases represent a group of chronic severe, disabling, and potentially fatal disorders of the skin and/or mucous membranes, primarily mediated by pathogenic auto-antibodies. Despite their rarity, these diseases are associated with significant morbidity and mortality and profound negative impact on the patient’s quality of life and impose a considerable economic burden. Rituximab, an anti-CD-20 monoclonal antibody, represents the first line of therapy for pemphigus, regardless of severity and a valuable off-label therapeutic alternative for subepidermal autoimmune blistering diseases as it ensures high rates of rapid, long-lasting complete remission. Nevertheless, disease recurrence is the rule, all patients requiring maintenance therapy with rituximab eventually. While innate resistance to rituximab in pemphigus patients is exceptional, acquired resistance is frequent and may develop even in patients with initial complete response to rituximab, representing a real challenge for physicians. We discuss the various resistance mechanisms and their complex interplay, as well as the numerous therapeutic alternatives that may be used to circumvent rituximab resistance. As no therapeutic measure is universally efficient, individualization of rituximab treatment regimen and tailored adjuvant therapies in refractory autoimmune blistering diseases are mandatory.
Metabolic Dysfunction-Associated Steatotic Liver Disease in Patients with Inflammatory Bowel Diseases: A Pilot Study
Authors: Abenavoli Ludovico , Spagnuolo Rocco , Scarlata Giuseppe Guido Maria , Gambardella Maria Luisa , Boccuto Luigi , Méndez-Sánchez Nahum , Luzza Francesco
Published: September 2024

Background: Inflammatory bowel disease (IBD) is characterized by persistent inflammation and is often associated with metabolic dysfunction-associated steatotic liver disease (MASLD). IBD patients are at risk of developing MASLD due to shared risk factors such as gut dysbiosis and systemic inflammation. The new MASLD nomenclature emphasizes the link between liver steatosis and cardiometabolic comorbidities. However, the prevalence of MASLD in IBD patients remains poorly explored. The main aim of this cross-sectional study is to assess the prevalence of ultrasound (US) and the clinical features of MASLD in patients with IBDs. Materials and Methods: We conducted a retrospective study enrolling 272 Italian IBD patients attending Renato Dulbecco Teaching Hospital in a period between 1 January 2021 and 31 December 2023. MASLD was diagnosed based on the presence of liver steatosis with cardiometabolic risk factors, using established guidelines. Demographic, clinical, and laboratory data were collected and analyzed. Statistical significance was determined at a p-value < 0.05. Results: Of the 272 IBD patients, 6% had non-alcoholic fatty liver disease (NAFLD), while 18% had MASLD. Patients with IBD-MASLD were significantly older, had higher body mass index, waist circumference, and triglyceride levels, and were more likely to have type 2 diabetes mellitus and hypertension compared to those with IBD-NAFLD. IBD-MASLD patients also showed higher disease activity scores and required more frequent surgical interventions. Bivariate logistic regression revealed triglyceride levels as a significant predictor of MASLD in IBD patients. Conclusions: MASLD is more prevalent in IBD patients, highlighting the importance of early detection of liver steatosis in this at-risk population. The association between MASLD and cardiometabolic risk factors underscores the need for a multidisciplinary approach to manage these patients effectively. Further studies in larger cohorts are necessary to confirm these findings and explore the pathophysiological mechanisms involved.