bluuebunny/crossref_metadata_2025 huggingface dataset:
Purification of a Src family tyrosine protein kinase from bovine retinas
Authors: Perdomo Deisy , Bubis José
Published: November 2020

Since tyrosine phosphorylation appears to play important functions in photoreceptor cells, we searched here for retinal nonreceptor tyrosine kinases of the Src family. We demonstrated that Src family tyrosine kinases were present in the cytosolic fraction of extracted bovine retinas. A Src family tyrosine kinase with an apparent molecular mass of about 62 kDa was purified to homogeneity from the soluble fraction of dark-adapted bovine retinas after three consecutive purification steps: ω-aminooctyl-agarose hydrophobic chromatography, Cibacron blue 3GA-agarose pseudo-affinity chromatography, and α-casein-agarose affinity chromatography. The purified protein was subjected to N-terminal amino acid sequencing and the sequence Gly-Ile-Ile-Lys-Ser-Glu-Glu was obtained, which displayed homology with the first seven residues of the Src family tyrosine kinase c-Yes from Bos taurus (Gly-Cys-Ile-Lys-Ser-Lys-Glu). Although the cytosolic fraction from dark-adapted retinas contained tyrosine kinases of the Src family capable of phosphorylating the α-subunit of transducin, which is the heterotrimeric G protein involved in phototransduction, the purified tyrosine kinase was not capable of using transducin as a substrate. The cellular role of this retinal Src family member remains to be found.
Alternative splicing coupled mRNA decay shapes the temperature‐dependent transcriptome
Authors: Neumann Alexander , Meinke Stefan , Goldammer Gesine , Strauch Miriam , Schubert Daniel , Timmermann Bernd , Heyd Florian , Preußner Marco
Published: November 2020

Mammalian body temperature oscillates with the time of the day and is altered in diverse pathological conditions. We recently identified a body temperature‐sensitive thermometer‐like kinase, which alters SR protein phosphorylation and thereby globally controls alternative splicing (AS). AS can generate unproductive variants which are recognized and degraded by diverse mRNA decay pathways—including nonsense‐mediated decay (NMD). Here we show extensive coupling of body temperature‐controlled AS to mRNA decay, leading to global control of temperature‐dependent gene expression (GE). Temperature‐controlled, decay‐inducing splicing events are evolutionarily conserved and pervasively found within RNA‐binding proteins, including most SR proteins. AS‐coupled poison exon inclusion is essential for rhythmic GE of SR proteins and has a global role in establishing temperature‐dependent rhythmic GE profiles, both in mammals under circadian body temperature cycles and in plants in response to ambient temperature changes. Together, these data identify body temperature‐driven AS‐coupled mRNA decay as an evolutionary ancient, core clock‐independent mechanism to generate rhythmic GE.
RESEARCH OF HYDROPHILIC GLOBULES CONTAINING A LACTIC ACID-CHITOSAN COMPLEX
Authors: Małolepsza-Jarmołowska Katarzyna
Published: November 2020

Gynecological scientific journals report that traditional therapeutic regimens used to treat vaginal inflammation recommended by global treatment centers and health organizations are not producing satisfactory results. Insufficient duration of the drug’s contact with vaginal mucosa does not provide adequate pH conditioning the physiological biocenosis of the organ. The conducted research and obtained positive results encourage further research possibilities in the field of optimization of pharmaceutical properties of the examined gynecological preparations. Assuming the above assumptions tested impact of polyvinylpyrrolidone K-15, polyvinylpyrrolidone K-30 and polyvinylpyrrolidone K-90 in the properties of the globules. The study was prepared formulations with different pH and rheological properties. The device simulating the conditions in the vagina, studied the adhesion and movement of the gel on the mucosa of the organ. Globules after application of the apparatus simulates the natural conditions in the gel passes and cover the surface. A wide range of pH of the gels allows the selection of the optimum formulation
SYNTHESIS AND CHARACTERIZATION OF CHITOSAN/SODIUM ALGINATE BLEND MEMBRANE FOR APPLICATION IN AN ELECTROCHEMICAL CAPACITOR
Authors: Nowacki Krzysztof , Galiński Maciej , Stępniak Izabela
Published: November 2020

In this work, we report a stepwise formation method of a chitosan/sodium alginate polyelectrolyte complex (CS/SA PEC) membrane. The proposed method aiming at the utilization of the ultrasonic treatment of chitosan and sodium alginate solution allowed us to obtain a highly homogeneous hybrid membrane for electrochemical usage. The CS/SA PEC membrane saturated in a 2 M Li2SO4 aqueous solution was used in electrochemical double layer capacitor (EDLC) cell to study its applicability as quasi-solid electrolyte. Electrochemical characteristic of EDLC cells was determined by electrochemical impedance spectroscopy, cyclic voltammetry and galvanostatic charge/discharge methods. The results show that the EDLC cell with CS/SA PEC quasi-solid electrolyte exhibit a comparable specific capacitance (102 F g-1 for 0–0.8 V) to CS reference (100 F g-1 for 0–0.8 V) and commercial separator (99 F g-1 for 0–0.8 V) cells. Thus, the CS/SA PEC membrane can be considered as an alternative modification for chitosanbased materials of electrochemical purpose.
PREPARATION AND CHARACTERIZATION OF CHITOSAN-AGAR FILMS
Authors: Wolska Joanna , Setkowicz Joanna , Maliszewska Irena H.
Published: November 2020

Hydrogel films composed of chitosan and agar or chitosan, agar and honey/bee pollen were prepared; they could be used as active wound dressings. During this study, various types of gel films were prepared using chitosan with different molecular weights and ratios of the two biopolymers. In addition, compositions with different amounts of honey or bee pollen were obtained. The selected samples were characterized by determining typical properties that are important for wound dressings. The best twocomponent films had a chitosan to agar weight ratio of 2:1. From among the examined tri-component films, the 2:1:0.5 weight ratio of chitosan,
agar and 50 wt% honey solution was the best composition.
Desain dan Implementasi Algoritma Korelasi Daya dan Tegangan di Kapasitor untuk Maximum Power Point Trackking pada Photovoltaic Menggunakan Arduino Uno
Authors: Pratomo Leonardus Heru , Riyadi Slamet
Published: November 2020

Photovoltaic is a main device in solar power generation systems. The performance of this component needs to be improved to get the maximum power point. Many methods for maximizing power in photovoltaic have been developed and implemented in various algorithms. In fact, the algorithm has a lot of complexity and it requires a signal processing system that has a high speed and expensive one in the implementation. To overcome this problem, an algorithm based on power and voltage correlation inthe capacitors is developed to get maximum power. If the voltage across the capacitor is controlled according to the maximum power point voltage (VMPP), the maximum power (PMPP) value would be achieved. This concept is verified through computational simulations anda voltage control algorithm would be implemented using the Arduino Uno microcontroller for the final verification. The efficiency of the simulation is 99.88%–99.92% and an experimental is 92.84%–94.78%.
Penerima Sinyal Emergency Locator Transmitter dengan Metode Direct Receiver pada Frekuensi 121,5 MHz
Authors: Rustamaji Rustamaji , Sawitri Kania , Hakim Arief Shubhan
Published: November 2020

To find out the location of an aircraft accident from an ELT signal beam of 121.5 MHz, search and rescue (SAR) officers or civil aviation authorities need an ELT signal receiver device that works at the same frequency, which is 121.5 MHz. The purpose of this research is to produce an ELT signal receiver with direct receiver method at a frequency of 121.5 MHz which is simpler, requires fewer RF devices, and is according to specifications for civil aviation. In this research, the design and realization of ELT signal receivers are carried out using the direct receiver method, including: a BPF and a RF amplifier as a direct receiver circuit, an AM demodulator, and an audio amplifier. Based on the results of measurements and tests that have been done, the direct receiver circuit can receive an ELT signal at a frequency of 121.5 MHz in the range of 121.330 to 121.617 MHz with -40.35 dB sensitivity, the AM demodulator can demodulate the audio signal at a frequency of 346.6 Hz, and the audio signal at a frequency of 337.6 Hz as a pointer to an ELT signal can be heard through the loudspeaker. The results of this study are ELT signal receivers with direct receiver method at a frequency of 121.5 MHz which are according to specifications for civil aviation.
PENGEMBANGAN MEDIA PEMBELAJARAN MENGGUNAKAN REKAMAN SIARAN RADIO BERBASIS KONTEKSTUAL SEBAGAI MATERI DIALOG BERBAHASA JAWA
Authors: Susanti Ulvah
Published: November 2020

ABSTRAK

Penelitian ini bertujuan (1) mendeskripsi analisis kebutuhan media pembelajaran menggunkan rekaman siaran radio berbasis kontekstual sebagai materi dialog berbahasa Jawa untuk siswa SMP, (2) mendeskripsi prototipe pengembangan media pembelajaran menggunakan rekaman siaran radio berbasis kontekstual sebagai materi dialog berbahasa Jawa untuk siswa SMP, (3) mendeskripsi validasi produk pengembangan media pembelajaran menggunkan rekaman siaran radio berbasis kontekstual sebagai materi dialog berbahasa Jawa untuk siswa SMP. Desain penelitian ini adalah penelitian pengembangan (Research and Development), prosedur penelitian meliputi (1) potensi dan masalah, (2) pengumpulan data, (3) desain produk, (4) validasi produk, dan (5) revisi produk. Penelitian yang dihasilkan yaitu media pembelajaran audio dialog berbahasa Jawa berupa CD atau soft file yang berisi 3 buah rekaman dialog untuk contoh dialog, 7 buah rekaman dialog untuk latihan soal, dan 7 buah rekaman dialog untuk soal ujian pemahaman mendengarkan. Media pembelajaran yang dihasilkan telah mendapat validasi dari ahli materi dan ahli media. Penilaian dan uji coba tersebut memberikan hasil bahwa media pembelajaran menggunakan rekaman siaran radio berbasis kontekstual materi dailog berbahasa Jawa layak digunakan sebagai media pembelajaran siswa kelas VIII SMP.

 

Kata kunci: media, dialog bahasa Jawa, kontekstual.

This study aims (1) to describe the contextual radio media needs analysis in Javanese dialogue learning for junior high school students, (2) to describe the prototype of contextual radio media development in Javanese dialogue learning for junior high school students, (3) describe the validation of radio media development products contextually based in Javanese dialogue learning for middle school students. The design of this study is research and development, research procedures include (1) potential and problems, (2) data collection, (3) product design, (4) product validation, and (5) product revisions. The research produced is Javanese audio dialogue learning media in the form of CD or soft file containing 3 dialogues for example dialogue, 7 dialogues for problem training, and 7 dialogues for listening comprehension exam questions. The learning media produced have received validation from material experts and media experts. The assessment and trial results showed that the learning media used radio broadcast recordings based on Javanese-language dailog material worthy of being used as a learning medium for junior high school eighth grade students.

Keywords:  media, javanese dialogue, contextual.
DIMENSI KONTESKTUAL PADA BAHAN AJAR GURU BERDASARKAN KURIKULUM 2013 DALAM MATA PELAJARAN BAHASA JAWA JENJANG SMA/SMK DI PURWOKERTO
Authors: Prihastuti Dani
Published: November 2020

Dani Prihastuti

Ucik Fuadhiyah, S.Pd.,M.Pd.

Mujimin, S.Pd.,M.Pd.

Program Studi Pendidikan Bahasa dan Sastra Jawa, FBS, UNNES

E-mail: prihastutidani@gmail.com

 

ABSTRACT

      This study aims to describe the contextualization of teacher teaching materials based on the 2013 curriculum in Javanese / high school / vocational language subjects in Purwokerto. The approach used in this study is a descriptive qualitative approach. Data collection techniques in this study are document review and interviews. The data source in this study is in the form of text studies. The instruments in this study used content analysis techniques. Based on the analysis, the majority of teaching materials used are printed teaching materials namely panginyongan textbooks, magazines and modules and given material from other sources originating from the internet. The results of the analysis show that from fifteen basic competencies, which meet the contextual criteria there are nine basic competencies. In basic competencies that meet contextual criteria, eighteen contextual teaching materials are obtained. The basic competencies included in the criteria for personal contestation are four and there are seven contextual teaching materials. Furthermore, there are five basic competencies and five contextual teaching materials. In cultural contextual criteria there are five basic competencies and six teaching materials that meet contextual criteria.

  Keyword: 2013 curriculum contextual dimensions, teaching materials

ABSTRAK

Penelitian ini bertujuan untuk mendeskripsikam wujud kekontekstualan bahan ajar guru berdasarkan kurikulum 2013 dalam mata pelajaran bahasa Jawa jenjang SMA/SMK di Purwokerto. Pendekatan yang digunakan dalam penelitian ini adalah pendekatan kualitatif deskriptif. Teknik pengumpulan data dalam penelitian ini adalah telaah dokumen dan wawancara. Sumber data dalam penelitian ini adalah berupa kajian teks. Instrumen dalam penelitian ini menggunakan teknik analisis isi (content analysis). Berdasarkan analisis, bahan ajar yang mayoritas digunakan adalah bahan ajar cetak yakni buku paket, majalah dan modul panginyongan serta diberikan materi dari sumber lain yang berasal dari internet. Hasil analisis menunjukan dari lima belas kompetensi dasar, yang memenuhi kriteria kotekstual ada sembilan kompetensi dasar. Pada kompetensi dasar yang memenuhi kriteria kontekstual, didapatkan delapan belas bahan ajar yang kontekstual. Kompetensi dasar yang termasuk dalam kriteria kontestual pribadi ada empat dan terdapat tujuh bahan ajar yang kontekstual. Selanjutnya, kriteria kontesktual sosial terdapat lima kompetensi dasar dan lima bahan ajar yang kontekstual. Pada kriteria kontekstual budaya terdapat lima kompetensi dasar dan enam bahan ajar yang memenuhi kriteria kontekstual.

Kata Kunci : dimensi kontekstual, kurikulum 2013,bahan ajar.
Pengembangan Buku Cerita Bergambar Berdasarkan Lagu–Lagu Berbahasa Jawa sebagai Media Penanaman Nilai Karakter Anak Usia Dini di Kabupaten Pemalang
Authors: Wati Ira Pergiwo , Sukoyo Joko , Widodo Widodo
Published: November 2020

Character education is very important, especially in childhood is an effective time for initiating the introduction and construction of traditional art. Based on observation, researchers found the problem, which is the lack of availability of the media that is able to attract the interest of students in learning Java language songs. Besides, children don’t understand Javanese Songs. The researchers had the idea to try to develop an interesting book, creative and innovative that is picture storybook based on Javanese language songs as a media to offer value character for children. In addition, that book can be used as cultural preservation efforts through appreciation. This is research and development (R&D). The data collection techniques used were documentation, observation, and question form. This book contains two main characters, there are safeguarding the environment and independently. This book contains 22 pages and it developed two stories containing character values.
Pengembangan Multimedia Interaktif Pembelajaran Membaca Teks Berhuruf Jawa untuk Siswa Kelas VII SMP di Kecamatan Ungaran Timur
Authors: Arum Fajar , Sudi Utami Esti , Kurniati Endang
Published: November 2020

Grade VII junior high in Ungaran Timur was bored with learning to read the text in a monotonous Java. This is due to gurus only use the textbook so that interest students in reading the text in Java is less. Therefore, the purpose of this research is to develop an interactive multimedia learning reading text in Java. The methods used in this research, namely research and development (R and D). The results of this research in the form of a prototype interactive multimedia learning reading text in Java. The prototype drew up based on the needs of students and teachers. The shape of the prototype in the form of interactive multimedia applications. Lettered text impressions of Java through a picture story and accompanying audio as a confirmation. The tested product design validation by expert media, material, and teachers as users. After the repair of the product design in accordance with the suggestions of the validator, interactive multimedia learning reading text in Java considered worthy to be used by students of Class VII junior high in Ungaran Timur.
Does On-Pump Beating Heart Endoaneurysmorrhaphy Have Advantages over Linear Aneurysmectomy on Arrested Heart in the Short Term?
Authors: Bugra Onursal , Daglar Bahadir
Published: November 2020

Background: It is still controversial which left ventricular aneurysm repair technique is optimal in terms of early and late results. This study aimed to compare early postoperative outcomes for 2 surgical treatments of postinfarction left ventricular aneurysm: linear repair technique on arrested heart versus endoaneurysmorrhaphy repair with patch plasty on beating heart.
Methods: Prospectively collected data from 16 consecutive patients who underwent endoaneurysmorrhaphy repair with patch plasty on beating heart (the technique we have preferred since 2008) were compared with data from a retrospective series of 10 patients who underwent linear repair on arrested heart (the technique we preferred until 2008). All operations were performed under elective conditions.
Results: Baseline characteristics of the 2 groups were similar. Complete revascularization for all diseased vessels was achieved in all patients. Durations of cross clamping, cardiopulmonary bypass, intensive care stay, and hospital stay were longer, and postoperative ejection fraction was lower, in the linear repair group compared with the endoaneurysmorrhaphy group (P < .05 for all). Early mortality occurred in 1 patient (3.8%) in the linear repair group.
Conclusion: Endoaneurysmorrhaphy repair with patch plasty on beating heart seems to offer advantages over the linear repair technique on arrested heart in the treatment of left ventricular aneurysms. Future large-scale prospective studies with longer follow-up are warranted to draw firm conclusions.
El agua en la dinámica de los recursos naturales estratégicos en un mundo volátil, incierto, complejo y ambiguo
Authors: Battaleme Juan Erardo
Published: November 2020

La idea del agua como recurso natural estratégico de la política internacional no es nueva, sin embargo desde la primera decada del presente siglo, el acceso a la misma ha cobrado especial intensidad. El presente paper pone en valor la idea de agua como recurso mediante el concepto de “Hidropolítica”, al separar el mito de las llamadas “guerras por el agua” de la realidad geopolitica que trasunta al recurso. Al enfatizar en la dinámica conflictiva y en especial en la aspiración de las grandes potencias para hacerse del recurso, dejan marginada la discusion al entender del autor mas apremiante, que es identificar como el recurso afecta los procesos de la politica regional y global de manera diferenciada a partir de ponderaciones completamente distintas entre los actores alrededor del recurso. Al mirar al agua, y siguiendo las premisas del realismo, existe una mayor probabilidad que se produzcan conflictos en torno a la misma a partir del uso compartido, que por la intervención directa de un actor extraregional para tomar el control de la misma. El presente trabajo tiene por objetivo, discutir cuando un RNE es considerado factor de conflicto armado ya sea inter o intraestratal, para luego trasladar esa dinamica a la hidropolitica a los efectos si su importancia relativa hace o no que la dinamica en torno a ella sea diferente a la que existe alrededor de otros recursos. En este sentido esperamos demostrar al menos en las discusiones existentes y con la evidencia que muestra el relevamiento sobre lo reflexionado al respecto, es que en relación al agua, la potencialidad existente por los juegos de suma cero, parecen ceder su lugar a construir regimenes internacionales que administren o encapsulen el conflicto. Si en un período determinado aparecen lecturas o percepciones que hacen temer por un futuro donde la escaces provoque conflictos, el cambio tecnológico y la arquitectura institucional permite contemplar un espacio para la resolución de las divergencias que puedan surgir, sin que “la sangre llegue al rio”.
Zakat Management Instutition: Management and Strategy
Authors: Melis Melis , Choiriyah Choiriyah , Saprida Saprida
Published: November 2020

The distribution of zakat is based on priority scale by paying attention to the principles of equity, justice and territoriality. In addition to receiving zakat (managing zakat), BAZNAS can receive and distribute donations, alms, and other religious social funds. The distribution and utilization of donations, alms, and other religious social funds are carried out in accordance with Islamic law and are carried out in accordance with the intended purpose of the giver. The management of donations, alms, and other religious social funds must be recorded in a separate bookkeeping. Here the importance of reports by BAZNAS, provincial BAZNAS, and Regency / City BAZNAS which contains the accountability and performance of the management of Zakat, infaq, alms and other religious social funds. ZIS managers are not only BAZNAS but there are also LAZ. This article will describe the management and management strategies of ZIS in detail.Keywords: ZIS, BAZNAS, LAZ AbstrakPendistribusian zakat dilakukan berdasarkan skala prioritas dengan memperhatikan prinsip pemerataan, keadilan, dan  ke wilayahan. Selain menerima zakat (mengelola zakat), BAZNAS dapat menerima dan mendistribusikan infak, sedekah, dan dana sosial keagamaan lainnya. Pendistribusian dan pendayagunaan infak, sedekah, dan dana sosial keagamaan lainnya tersebut dilakukan sesuai dengan syariat Islam dan dilakukan sesuai dengan peruntukkan yang diikrarkan oleh pemberi.Pengelolaan infak, sedekah, dan dana sosial keagamaan lainnya harus dicatat dalam pembukuan tersendiri. Disini pentingnya laporan oleh BAZNAS, BAZNAS provinsi, dan BAZNAS Kabupaten/Kota yang memuat akuntabilitas dan kinerja pelaksanaan Pengelolaan Zakat, infak, sedekah, dan dana sosial keagamaan lainnya. Pengelola ZIS tidak hanya BAZNAS tetapi juga ada LAZ. Artikel ini akan memaparkan manajemen dan strategi pengelolaan ZIS secara detail.Kata Kunci: ZIS, BAZNAS, LAZ
Effects of elevated carbon dioxide on arbuscular mycorrhizal fungi activities and soil microbial properties in soybean (Glycine max L. Merrill) rhizosphere
Authors: Adeyemi Nurudeen Olatunbosun
Published: November 2020

Arbuscular mycorrhizal fungi (AMF) help in promoting plant growth and mediating key belowground processes, however, AMF responses to the continuous increase in the atmospheric carbon dioxide (CO2) is yet elusive. This has led to considerable interest in the impacts elevated CO2 on AMF and belowground processes in recent years. The present study investigated the effect of elevated CO2 on AMF sporulation and root colonization and soil microbial properties in the rhizosphere of soybean. The pot experiment consisted of two levels of CO2 (ambient; 350 ppm and elevated; 550 ppm) and three soybean cultivars (TGx 1440-1E, TGx 1448-2F and TGx 1480-2F) conducted in open top chambers, laid out in randomized complete block design, replicated thrice. The results showed that elevated CO2 increased the AMF spore density and root colonization of the soybean cultivars. Elevated CO2 increased the microbial biomass carbon (34.2–45.4%), microbial biomass nitrogen (44.6–54.9%), soil nitrogen (30.3–50.6%), available phosphorus (20.8–45.7%) in the rhizosphere of the soybean cultivars compared to the ambient CO2 . These could have resulted in increased plant biomass, pod number, 100-seed weight and seed yield under elevated CO2 . From the results of this study, increased atmospheric CO2 regulates AMF activities, microbial properties and improve soybean performance. Thus, this study may help to a better understanding of the responses of AMF and belowground process with increasing atmospheric CO2.
Changes in production and reproduction traits in population of the Slovak Spotted Cattle
Authors: Bujko Jozef , Candrák Juraj , Žitný Július , Kasarda Radovan
Published: November 2020

The study aimed to examine the analyses of the trend in the numbers of dairy cows, production and reproductive traits in the population the Slovak Spotted cattle for period 2010 to 2019. The number of Slovak Spotted dairy cows is maintained on a relatively stable level in recent years. There was a decrease in the number of animals (12 428 in 2010), but not as strong as after 1990 (85 518). From 2017 number of animals is rising from 14,062, to 14,150 (2018) and 14,627 (2019), respectively. As compared to 2010 an increase of 13.15% in 2017, 13.86% and 17.68%, in 2018 and 2019 respectively was observed. Population size raised from 113.15% (2017) to 117.68% in 2019 respectively. The average annual increase in milk production between the years 2010 to 2019 was + 157.5 kg of milk (total 1,575 kg of milk), + 5.8 kg of fat (total 58 kg of fat) and + 6.1 kg of protein (total 61 kg of protein). Positive growth of milk production in recent years is slightly comparable with the trend of breeding improvement.
Genetic and phenotypic trends for weights of major beef and dual-purpose cattle breeds in Slovak Republic
Authors: Tomka Ján , Huba Ján , Kumičík Milan
Published: November 2020

Genetic evaluation of beef cattle in Slovakia started in first years of this century. After the first decade of running the routine evaluations it is important to review the progress made and to discuss the further development. The aim of this paper was to compile and deliver genetic and phenotypic trends in order to review importance of evaluated traits with respect to four major beef (Charolais, Limousine) and dual-purpose breeds (Slovak Pinzgau cattle, Slovak Spotted cattle). The study showed different progress made across the breeds. Higher genetic progress was observed in Charolais purebreds compared to Limousine counterparts. Moreover, almost similar progress to that in Charolais was observed in dual-purpose Slovak Pinzgau cattle. On the other hand no progress was observed in Slovak Spotted purebreds. Results also showed higher progress in bulls compared to cows. Phenotypic trends showed reserves for improvement in Charolais and closing to breed standards in Limousine. In dualpurpose breeds improvement was shown only in Slovak Pinzgau, while no improvement or decrease in actual weights was shown in Slovak Spotted purebreds.
The high-throughput screening system for inhibitor Mycobacterium tuberculosis compounds based on ATP hydrolysis activity of recombinant protein ClpC1
Authors: Duc Nguyen Minh , Lee Hanki , Won Suh Joo , Bich Thuy Vo Thi , Ngoc Minh Nghiem , Phan Lan Hong Nguyen
Published: November 2020

The global Tuberculosis (TB) rate continues to increase by 1% per year with the widespread of drug-resistant TB. Therefore, the development and research to find new anti-TB drugs are becoming an extremely urgent mission. To be able to screen lead anti-tuberculosis drugs, currently, researchers have to carry out directly on the cells of Mycobacterium tuberculosis and to be performed in bio-security facilities level 3 or 4, to prevent infection from pathogens. However, our results demonstrated that the screening of anti-TB drug candidates can be implemented in bio-security facilities level 1 laboratory with the Escherichia coli cell extraction and recombinant ClpC1 protein - an integral part of the Mycobacterium tuberculosis genome. We focused on the ATP hydrolysis activity of ClpC1 to create a specific research direction for the high-throughput anti-tuberculosis drug screening system. ClpC1 protein was overexpressed and purified with functionally characterized (93.5 kDa). The steady-state growth of recombinant ClpC1 protein in Luria-Bertani (LB) broth High Salt medium was maintained and stabilized after extraction. The determined ATPase activity of ClpC1 was performed by measuring the released phosphate from the reaction. Ecumicin was chosen to be a control compound with expected ATP hydrolysis activities (Hill coefficient = 1,19 ± 0,217; Kd value = 0,52 ± 0,275). We tested this high throughput screening system with ten anti-TB drugs to evaluate the effectiveness of our screening system. Based on these results, we built a complete high-throughput screening system anti-tuberculosis drug safety and quickly.
Effects of in vitro maturation media on in vitro fertility of porcine oocytes and early development of embryos
Authors: Viet Linh Nguyen , Thi Hiep Nguyen
Published: November 2020

In pigs, embryo productivity is still lower than that in other livestocks. One of the reasons is incomplete maturation of porcine oocytes in in vitro conditions. Therefore in vitro maturation (IVM) plays a crucial role in in vitro production of porcine embryos. It provides prerequisite condition to in fertilization and subsequent development of porcine embryos. In a previous study, effects of NCSU-37-based medium and TCM-199-based media supplemented with porcine follicular fluid (pFF) or Fetal Bovine Serum (FBS) on in vitro maturation of Landrace oocytes collected in Vietnam have been compared, suggesting that NCSU-37 medium supplemented with 10% of porcine follicular fluid (pFF) had the highest rate of oocytes reach to metaphase II stage in comparison to those of the other two TCM-199-based media. In the present study, further experiments were carried out to evaluate the contribution of IVM media on fertilization capability and developmental competence. Porcine oocytes matured in vitro in 3 media: NCSU-37 supplemented with 10% pFF, TCM-199 supplemented with either 10% pFF or 10% FBS were subjected to in vitro fertilization and subsequent in vitro culture to monitor fertility and embryo development. The results showed that penetration and normal fertilization rates in both TCM-199 groups are both higher than that of NCSU-37 group. Moreover, the cleavage and blastocyst rates, and cell numbers of blastocysts which is a criterion for embryo quality were all higher in TCM-199 groups, especially in the group supplemented with pFF. It might be concluded that TCM-199 media supplemented with either pFF or FBS are suitable for effective in vitro maturation of Landrace porcine oocytes collected in Vietnam.
Effect of gamma irradiation on the viability and cellulase production of some filamentous fungi
Authors: Diep Tran Bang , Thi Thom Nguyen , Dang Sang Hoang , Xuan An Tran , Van Binh Nguyen , Minh Quynh Tran
Published: November 2020

The motivation of our research is to examine the mutagenic effect of gamma irradiation on cellulase secretion of some filamentous fungi. The spore suspensions of Aspergillus sp. TTG and Trichoderma sp. VTCC were irradiated at dose ranging 0-2500 Gy under gamma Cobalt-60 source at Ha Noi Irradiation Center. The result showed that the survival rate of fungi decreases with the increasing dose. The radiation dose required to kill 90% of the total number of fungal spores (D10) of these strains was about 400 Gy. The viability of Aspergillus sp. TTG and Trichoderma sp. VTCC at 500 Gy were 0.46 % and 0.78%, respectively, while the number of survival spore decreased by 6.5-7.5 Log unit at the dose of 2500 Gy. By screening in PDA medium with the addition of CMC (carboxymethyl cellulose) and Congo red as an indicator of cellulose degradation, hundreds of colonies with higher hydrolysis capacity’s value (HC) compared to the initial strain were observed after irradiation. The colonies expressed the highest cellulose hydrolysis capacity with maximum HC value were obtained at dose range of 700-1500 Gy. It is important to notice the 5 potential mutants including 3 mutants of Aspergillus (TTG-700, TTG-1000 and TTG-1200) and 2 mutants of Trichoderma (VTCC-1000, VTCC-1500) demonstrated the higher CMCase secretion (1.78 – 2.48 times) compared to the wild types. After 5 generations, the enzyme productions of the mutants were fairly stable and there were no differences in growth rates and morphology of each generation. The result of this study is an evidence for using gamma irradiation to improve cellulase production in filamentous fungi.
Inhibited growth of mesenchymal stem cells under simulated microgravity
Authors: Quynh Chi Ho Nguyen , Quang Huy Hoang Nguyen , Chinh Chung Doan , Nghia Son Hoang , Thanh Long Le
Published: November 2020

This study aimed to estimate the effects of simulated microgravity (SMG) on mesenchymal stem cells (MSCs). The 3D clinostat was applied to induce to simulated microgravity on MSC. The results showed that the MSC density in control group was higher than the SMG group. The cell cycle analysis demonstrated that the MSC ratio in G0/G1 phase in SMG group was higher than that of the control group, while the MSC ratio in S phase and G2/M phase in SMG group was lower than those of the control group. The real time quantitative RT-PCR was used to evaluate the expression of cell cycle-related genes, including Cyclin-dependent kinase 2 (Cdk2), Cyclin-dependent kinase 6 (Cdk6), and Cyclin A. The results showed that the transcript expression of Cdk2, Cdk6, and Cyclin A was down-regulated in MSC from SMG group comparing to that of the control group. The flow cytometry was performed to determine the ratio of apoptotic MSCs. There was no significant difference in viability ratio and apoptotic ratio of MSCs between SMG group and control group. The MSCs from SMG group and control group showed similar in Bcl2 and Bax transcript expression.
How Fantasy Speaks to Adolescent Readers
Authors: Smit Julie
Published: November 2020

Many genres of fictional novels are considered groundbreaking for complex plots and psychologically interesting characters. Little attention has been focused on how fantasy can be groundbreaking. This exploratory case study centers on how the five-novel series Percy Jackson & the Olympians, and its five-part sequel The Heroes of Olympus, speaks to a reading community of eighth grade female adolescent readers. This study traces the development of social inquiries of gender inequality, heroism and imperfection, and good and evil from these readers’ interactions with characters and events in the world of Percy Jackson.
Reproducibility for Thistlethwaite et al. 2020 v4
Authors: R Thistlethwaite Lillian , not provided Varduhipetrosyan
Published: November 2020

We consider the following algorithmic problem that arises in transcriptomics, metabolomics and other fields: given a weighted graph and a subset of its nodes, find subsets of perturbed variables that show significant connectedness within the graph. While solutions to this problem may be discovered by devising a variety of scoring functions, statistically rigorous discovery is obstructed by the high computational cost of permutation testing, required to establish p-values. In this work, we develop CTD, a novel information-theoretic method that evaluates the connectedness of perturbation signatures in a network context and assigns an upper bounds on their p-values without use of permutation testing. We apply CTD to interpret multi-metabolite perturbations due to inborn errors of metabolism and multi-gene perturbations associated with breast cancer in the context of disease-specific Gaussian Markov Random Field networks learned directly from respective molecular profiling data.
New Alliances in Post-Brexit Europe: Does the New Hanseatic League Revive Nordic Political Cooperation?
Authors: Schulz Daniel F. , Henökl Thomas
Published: November 2020

As Brexit removes the Nordic countries’ most powerful ally from the EU, what does this imply for their approach to European affairs? The literature on small states within the EU suggests that they can counterbalance limited bargaining capacities by entering two types of alliances: strategic partnerships with bigger member states and institutionalised cooperation on a regional basis. Against this backdrop we ask whether, by significantly raising the costs of non-cooperation for Nordic governments, the Brexit referendum has triggered a revival of Nordic political cooperation. We scrutinise this conjecture by analysing Nordic strategies of coalition-building on EU financial and budgetary policy, specifically looking at attempts to reform Europe’s Economic and Monetary Union and proposals to strengthen the EU’s fiscal powers. We find that Nordic governments have successfully collaborated on these issues in the context of new alliances such as the ‘New Hanseatic League’ or the ‘Frugal Four.’ Yet, their coalition-building strategies rely on relatively loose and issue-specific alliances rather than an institutionalisation of Nordic political cooperation, implying that this revival of Nordic political cooperation hardly involves the institutions of ‘official’ Nordic cooperation. We argue that this reflects lasting differences among the Nordics’ approach to the EU as well as electorates’ scepticism about supranational institution-building, implying that ‘reluctant Europeans’ are often also ‘reluctant Scandinavians.’
Numerical Method for Calculations of the Multi-Dielectric Fields Based on Flux Density in High Voltage Power Transformer Apparatus
Authors: PAMUK Nihat
Published: November 2020

This paper deals with a “combination method” in which the charge simulation method is combined with finite element method, for electric field calculation. The proposed method has the advantages of both charge simulation method and finite element method, while making up of their disadvantages. In the combination method, field is divided into two regions, one covered by the charge simulation method and the other by the finite element method. These two regions are combined using the continuous conditions for potentials and dielectric flux densities at the boundary. The new method could be applied to non-enclosed multi-dielectrics fields, space charge fields, fields with leakage current and so on. Examples of calculation have revealed that the method affords satisfactory calculation accuracy in application to high voltage power transformer apparatuses.
The Role of Imaginative Thinking in Scientific Thinking
Authors: Kulanin Y.D. , Stepanov M.E. , Nurkaeva I.M.
Published: November 2020

The article discusses the role of creative thinking in scientific thinking and in particular in the teaching of mathematics in higher educational institutions. From this point of view, the relationship between abstract analytical and visual geometric methods in mathematics is highlighted, and it is concluded that the latter form the basis for the application of creative thinking in mathematics.
Sema ve Arz Kavramlarının İşâri Tefsirlerdeki Yorumlarına İlişkin Bir Analiz
Authors: AYDIN Abdurrahman
Published: November 2020

Kur'an üzerine yapılan çalışmalarda kavramların derinlemesine ve geniş çaplı düşünülmemesi önemli bir eksikliktir. Çünkü "sema/semâvât" ve "arz" lafızlarıyla ilgili yapılan tefsirlerde olduğu gibi, Kur'an'ın derin manaları sınırlanabilmektedir. Kur’an’ın sembolik dili hakkındaki bilgi sahibi olmak, doğru mananın elde edilmesi açısından vazgeçilmezdir. Tefsirlerin geneli "sema/semâvât" ve "arz" lafızlarının üzerinde durmamış ve zâhiri manasıyla ele almışlardır. İşârî tefsirlerde ise, bu kavramların içinde bulunduğu âyetlerden, hem bireysel hem de toplumsal mesajlar çıkarılmıştır. Bunun sebebi işârî tefsir yazarlarının, âyetlerin dış dünyaya bakan yönünün yanında, insanın yaratılışı ile bağlantısının varlığından daha çok haberdar olmalarıdır. İşârî tefsirlerin önemli bir özelliği, âyetlerin yorumlanmasında insan psikolojisine dair mesajların yoğunluklu olmasıdır. Tefsirin bir amacı, Kur'anî kelime ve kavramların arka planındaki dünyayı keşfederek ordaki manaya nüfûz etmektir. "Kendini bilen rabbini bilir" hadisi ile uyumlu olarak mutasavvıflar, daha çok iç temizliği ve kalp saflığı gibi insanın derûnî dünyası ile ilgili konuları ön planda tutmuşlardır. Buna bağlı olarak işârî/tasavvufî tefsir yazarları, "sema/semâvât" ve "arz" lafızlarını yorumlarken, insanın içselliği ile daha çok ilişkilendirmişlerdir.
Molecular mechanisms of the cardiotoxic action of anthracycline antibiotics and statin-induced cytoprotective reactions of cardiomyocytes
Authors: Tursunova N.V. , Klinnikova M.G. , Babenko O.A. , Lushnikova E.L.
Published: November 2020

The manifestation of the side cardiotoxic effect of anthracycline antibiotics limits their use in the treatment of malignant processes in some patients. The review analyzes the main causes of the susceptibility of cardiomyocytes to the damaging effect of anthracyclines, primarily associated with an increase in the processes of free radical oxidation. Currently, research is widely carried out to find ways to reduce anthracycline cardiotoxicity, in particular, the use of cardioprotective agents in the complex treatment of tumors. Hydroxymethylglutaryl coenzyme A reductase inhibitors (statins) have been shown to improve the function and metabolism of the cardiovascular system under various pathological impacts, therefore, it is proposed to use them to reduce cardiotoxic complications of chemotherapy. Statins exhibit direct (hypolipidemic) and pleiotropic effects due to the blockade of mevalonic acid synthesis and downward biochemical cascades that determine their cardioprotective properties. The main point of intersection of the pharmacological activity of anthracyclines and statins is the ability of both to regulate the functioning of small GTPase from the Rho family, and their effect in this regard is the opposite. The influence of statins on the modification and membrane dislocation of Rho proteins mediates the indirect antioxidant, anti-inflammatory, endothelioprotective, antiapoptotic effect. The mechanism of statin inhibition of doxorubicin blockade of the DNA-topoisomerase complex, which may be important in preventing cardiotoxic damage during chemotherapy, is discussed. At the same time, it should be noted that the use of statins can be accompanied by adverse side effects: a provocation of increased insulin resistance and glucose tolerance, which often causes them to be canceled in patients with impaired carbohydrate metabolism, so further studies are needed here. The review also analyzes data on the antitumor effect of statins, their ability to sensitize the tumor to treatment with cytostatic drug. It has been shown that the relationship between anthracycline antibiotics and statins is characterized not only by antagonism, but also in some cases by synergism. Despite some adverse effects, statins are one of the most promising cardio- and vasoprotectors for use in anthracycline cardiomyopathy.
The expression level of cytokine genes in the cases of native heart valves in infectious endocarditis
Authors: Sinitsky M.Yu. , Tsepokina A.V. , Asanov M.A. , Kazachek Ya.V. , Evtushenko A.V. , Ponasenko A.V.
Published: November 2020

The expression level of IL1B, IL6, IL8, IL10, IL12A, IL12B, IL18, IL23, IL33, CCL2, and IL1RL1 has been investigated using biopsies of native mitral, aortic, and tricuspid valves obtained during surgical correction of acquired defect from 25 patients with infectious endocarditis. Biopsies of native mitral and aortic valve cusps from 12 patients who underwent surgical correction of acquired heart disease of non-infectious etiology were used as control. We used quantitative PCR with fluorescent dye SYBR Green for determination of the cytokine gene expression level. This study revealed that genes could be subdivided into three groups: (i) genes with increased expression (IL1B, IL6, and IL8); (ii) genes with reduced expression (IL33 and IL1RL1); (iii) genes with unchanged expression (IL12A, IL18, IL23, and CCL2). The IL8 gene expression was characterized by the most pronounced increase (9.83 times versus control), while the IL1RL1 gene demonstrated the most pronounced decrease in its expression (4.17 times). Expression of IL10 and IL12B genes was negligible in all samples.
Impact of incarceration on nutritional status and oral health among male inmates of central jail of Jaipur city, India
Authors: Sharma A , Parkar S , Gaur A , Bagri B
Published: November 2020

Aim: Prison is an especially difficult environment for promoting oral health and leads to the exacerbation of unhealthy behaviors. This study set out to assess the impact of incarceration on nutritional status and oral health among the male prison inmates of Central Jail of Jaipur city, Rajasthan, India. Material and method: A cross sectional study was conducted among 181 male inmates. Dental caries and periodontal status were assessed by using modified the Decayed Missing Filled Teeth (DMFT) index and Community Periodontal Index (CPI) and Loss of Attachment (LOA) index as per the WHO methodology 1997. Nutritional status was assessed by Body Mass Index (BMI) and weight (kg)/height (m2). Results: Out of 181 inmates, 141 (77.90%) had normal BMI. The majority (n=128, 70.70%) of inmates were incarcerated for less than 6 years. A significant difference (P<0.05) was found between age and periodontal status. A significant difference was observed (P<0.05) in the CPI score 3 and LOA 0 and 1 score. However, no significant difference (P>0.05) was observed in DMFT and period of incarceration. Age was the significant (P<0.001) risk factor for CPI and LOA. Discussion: Dental caries and periodontal health deteriorates with age and period of incarceration. Oral hygiene maintenance along with comprehensive oral care can be accomplished by establishing dental care facilities in prisons.
Euthanasia: trends and opinions in Spain
Authors: Bernal-Carcelén I
Published: November 2020

Objective: Analyse the evolution of opinions about euthanasia by the general public and clinical physicians from 1995 to 2019 and their influence at the present time. Material and method: Bibliographical review based on relevance and quality of publications in open access and academic access platforms. Main surveys review of public and private institutions. Congress and Senate Official Journal Sessions. Results: Recent surveys show that clinicians support the regulation of euthanasia. This position matches the general public’s belief, which has grown steadily in recent decades. Discussion: Social and clinical support for the regulation of euthanasia has been fundamental. In the last two decades political parties have changed their positions, thus creating a window of opportunity.