bluuebunny/crossref_metadata_2025 huggingface dataset:
Kriging Metamodeling in Rotordynamics: Application for Predicting Critical Speeds and Vibrations of a Flexible Rotor
Authors: Sinou J.-J. , Nechak L. , Besset S.
Published: March 2018

Rotating machinery produces vibrations depending upon the design of the rotor systems as well as any faults or uncertainties in the machine that can increase the vibrations of such systems. This study illustrates the effectiveness of using surrogate modeling based on kriging in order to predict the vibrational behavior (i.e., the critical speeds and the vibration amplitudes) of a complex flexible rotor in the presence of uncertainties. The basic idea of kriging is to predict unknown values of a function by using a small size set of known data. The kriging estimation is based on a weighted average of the known values of the function in the neighborhood of the point for which the value of the function has to be calculated. The crucial dependence of a kriging predictor versus the correlation functions and different orders will be illustrated. This paper also shows that reducing the number of samples required to have predictive models can be achieved by performing an initial understanding of the mechanical system of interest and by considering certain characteristics directly deriving from the physics of the problem studied.
Simulated Cholinergic Reinnervation ofβ(INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets ContainingβCell and Cholinergic Cell
Authors: Jiao Ao , Li Feng , Zhang Chengshuo , Lv Wu , Chen Baomin , Zhang Jialin
Published: March 2018

Cholinergic neurons can functionally support pancreatic islets in controlling blood sugar levels. However, in islet transplantation, the level of cholinergic reinnervation is significantly lower compared to orthotopic pancreatic islets. This abnormal reinnervation affects the survival and function of islet grafts. In this study, the cholinergic reinnervation of beta cells was simulated by 2D and 3D coculture of INS-1 and NG108-15 cells. In 2D culture conditions, 20 mM glucose induced a 1.24-fold increase (p<0.0001) in insulin secretion from the coculture group, while in the 3D culture condition, a 1.78-fold increase (p<0.0001) in insulin secretion from heterotypic pseudoislet group was observed. Glucose-stimulated insulin secretion (GSIS) from 2D INS-1 cells showed minimal changes when compared to 3D structures. E-cadherin expressed in INS-1 and NG108-15 cells was the key adhesion molecule for the formation of heterotypic pseudoislets. NG108-15 cells hardly affected the proliferation of INS-1 cells in vitro. Heterotypic pseudoislet transplantation recipient mice reverted to normoglycemic levels faster and had a greater blood glucose clearance compared to INS-1 pseudoislet recipient mice. In conclusion, cholinergic cells can promote insulin-secreting cells to function better in vitro and in vivo and E-cadherin plays an important role in the formation of heterotypic pseudoislets.
Human Genomic Loci Important in Common Infectious Diseases: Role of High-Throughput Sequencing and Genome-Wide Association Studies
Authors: Mboowa Gerald , Sserwadda Ivan , Amujal Marion , Namatovu Norah
Published: March 2018

HIV/AIDS, tuberculosis (TB), and malaria are 3 major global public health threats that undermine development in many resource-poor settings. Recently, the notion that positive selection during epidemics or longer periods of exposure to common infectious diseases may have had a major effect in modifying the constitution of the human genome is being interrogated at a large scale in many populations around the world. This positive selection from infectious diseases increases power to detect associations in genome-wide association studies (GWASs). High-throughput sequencing (HTS) has transformed both the management of infectious diseases and continues to enable large-scale functional characterization of host resistance/susceptibility alleles and loci; a paradigm shift from single candidate gene studies. Application of genome sequencing technologies and genomics has enabled us to interrogate the host-pathogen interface for improving human health. Human populations are constantly locked in evolutionary arms races with pathogens; therefore, identification of common infectious disease-associated genomic variants/markers is important in therapeutic, vaccine development, and screening susceptible individuals in a population. This review describes a range of host-pathogen genomic loci that have been associated with disease susceptibility and resistant patterns in the era of HTS. We further highlight potential opportunities for these genetic markers.
Effect of the Aggregate Size on Strength Properties of Recycled Aggregate Concrete
Authors: Kang Ma , Weibin Li
Published: March 2018

The study on preparation technology of recycled concrete with economical and technical feasibility has gained more serious attention in each country due to its involvement and effect on the environment protection and the sustainable development of human society. In this study, we conducted a control variable test to investigate and assess the influence of the aggregate size on the strength characteristics of concrete with different diameters of recycled aggregates. Concrete with recycled aggregates of 5∼15 mm (A), 15∼20 mm (B), 20∼30 mm (C), and their combinations were subjected to a series of unconfined pressure tests after curing for 28 days. Based on the results obtained from the tests, an effort was made to study the relationship between the mechanical characteristics of recycled aggregate concrete and aggregate particle size. Also, a regression model of recycled concrete was proposed to predict the elasticity modulus and to adjust the design of mixture proportion. It is believed that these experiment results would contribute to adjust the remediation mixture for recycling plants by considering the influence of recycled aggregate size.
Salivary Levels of Hemoglobin for Screening Periodontal Disease: A Systematic Review
Authors: Nomura Yoshiaki , Okada Ayako , Tamaki Yoh , Miura Hiroko
Published: March 2018

Periodontal disease is a common inflammatory disease. It affects about 20–50% of global population in both developed and developing countries. Early detection of slight changes of periodontal tissue plays an important role in prevention of onset and progression of periodontal disease. Hence, there is a need of a screening test to assess periodontal tissue for health check-ups. Salivary levels hemoglobin (Hb) has been proposed to assess the conditions of the inflammation of gingiva. The aim of this systematic review was to evaluate and summarize critically the current evidences for Hb as periodontal screening test. We performed a literature search of report published using PubMed databases. A total of 55 articles were retrieved and 16 were selected. Our review focuses on corelation coefficient with periodontal clinical parameters or sensitivity and specificity. As a result, fourteen studies calculated sensitivity and specificity of Hb. Six studies measured salivary levels hemoglobin at laboratory: three studies used polyclonal antibody reactions and other studies used colorimetric tests. Eight studies used paper strip method: 4 studies used monoclonal antibody reaction and 4 studies used colorimetric tests. Youden’s indexes by antibody reaction were better than those of colorimetric methods. Evidences are described above and further studies are necessary to set the cut off values stratified by gender, age and number of remaining teeth.
High PINCH1 Expression in Human Laryngeal Carcinoma Associates with Poor Prognosis
Authors: Tsinias Georgios , Nikou Sofia , Papadas Theodoros , Pitsos Panagiotis , Papadaki Helen , Bravou Vasiliki
Published: March 2018

Focal adhesion signaling to actin cytoskeleton is critically implicated in cell migration and cancer invasion and metastasis. Actin-binding proteins cofilin and N-WASP regulate actin filament turnover, and focal adhesion proteins parvins and PINCH mediate integrin signaling to the actin cytoskeleton. Altered expression of these proteins has been implicated in human cancer. This study addresses their expression and prognostic significance in human laryngeal carcinoma. Protein expressions of cofilin, N-WASP,α-parvin,β-parvin, and PINCH1 were examined by immunohistochemistry in 72 human laryngeal squamous cell carcinomas. Correlations with clinicopathological data and survival were evaluated. All proteins examined were overexpressed in human laryngeal carcinomas compared to adjacent nonneoplastic epithelium. High expression of PINCH1 was associated significantly with high grade, lymph node-positive, and advanced stage disease. Moreover, high PINCH1 expression significantly associated with poor overall and disease-free survival and high cytoplasmic PINCH1 expression was shown by multivariate analysis to independently predict poor overall survival. In conclusion, we provide novel evidence that focal adhesion signaling to actin cytoskeleton is implicated in human laryngeal carcinogenesis and PINCH1 has prognostic significance in the disease.
Effect of Molybdenum on the Corrosion Behavior of High‐Entropy Alloys CoCrFeNi2 and CoCrFeNi2Mo0.25 under Sodium Chloride Aqueous Conditions
Authors: Rodriguez Alvaro A. , Tylczak Joseph H. , Gao Michael C. , Jablonski Paul D. , Detrois Martin , Ziomek-Moroz Margaret , Hawk Jeffrey A.
Published: March 2018

The corrosion behavior of high‐entropy alloys (HEAs) CoCrFeNi2 and CoCrFeNi2Mo0.25 was investigated in 3.5 wt. percent sodium chloride (NaCl) at 25°C by electrochemical methods. Their corrosion parameters were compared to those of HASTELLOY® C‐276 (UNS N10276) and stainless steel 316L (UNS 31600) to assess the suitability of HEAs for potential industrial applications in NaCl simulating seawater type environments. The corrosion rates were calculated using corrosion current determined from electrochemical experiments for each of the alloys. In addition, potentiodynamic polarization measurements can indicate active, passive, and transpassive behavior of the metal as well as potential susceptibility to pitting corrosion. Cyclic voltammetry (CV) can confirm the alloy susceptibility to pitting corrosion. Electrochemical impedance spectroscopy (EIS) elucidates the corrosion mechanism under studied conditions. The results of the electrochemical experiments and scanning electron microscopy (SEM) analyses of the corroded surfaces revealed general corrosion on alloy CoCrFeNi2Mo0.25 and HASTELLOY C‐276 and pitting corrosion on alloy CoCrFeNi2 and stainless steel 316L.
Examination Stress Results in Attentional Bias and Altered Neural Reactivity in Test-Anxious Individuals
Authors: Zhang Xiaocong , Dong Yunying , Zhou Renlai
Published: March 2018

Examination stress occurs so frequently in the lives of students. The neural mechanisms of attentional bias induced by examination stress in test-anxious individuals remain unclear. Accordingly, we investigated the attentional bias toward test-related threatening words in selected high and low test-anxious participants under the stress of final examinations by using an event-related potential (ERP) technique. A classic dot-probe paradigm was adopted with a test-related/test-unrelated threatening word and a neutral word pair as cues. Results showed attention bias and enhanced N200 amplitude toward test-related threat in high test-anxious individuals, whereas avoidance of test-related threat and decreased N200 amplitude were shown in low test-anxious individuals. Additionally, ERP data revealed the relatively enhanced LPP amplitude in low test-anxious participants compared with that in high test-anxious participants. No attentional bias toward test-unrelated threat was found. In conclusion, examination stress resulted in attentional bias and functional perturbations of a brain circuitry that reacted rapidly to test-related threat in high test-anxious individuals.
In Vitro Lethal Activity of the Nematophagous Fungus Clonostachys rosea (Ascomycota: Hypocreales) against Nematodes of Five Different Taxa
Authors: Rodríguez-Martínez Rosalia , Mendoza-de-Gives Pedro , Aguilar-Marcelino Liliana , López-Arellano María Eugenia , Gamboa-Angulo Marcela , Hanako Rosas-Saito Greta , Reyes-Estébanez Manuela , Guadalupe García-Rubio Virginia
Published: March 2018

This study was aimed to evaluate the in vitro lethal activity of the nematophagous fungi Clonostachys rosea against 5 nematodes species belonging to different taxa. Two groups of 35 Petri dishes (PD) each were divided into 5 series of 7 (PD). Group 1 (series 1, 2, 3, 4, and 5) contained only water agar; meanwhile group 2 plates (series 6, 7, 8, 9, and 10) contained C. rosea cultures growth on water agar. Every plate from the two groups was added with 500 nematodes corresponding to the following genera/specie: Haemonchus contortus, Caenorhabditis elegans, Rhabditis sp., Panagrellus redivivus, and Butlerius sp. After 5-day incubation at room temperature, free (nontrapped) larvae were recovered from plates using the Baermann funnel technique. Recovered nematodes were counted and compared with their proper controls. Results shown an important reduction percentage of the nematode population attributed to the fungal lethal activity as follows: H. contortus (L3) 87.7%; C. elegans 94.7%; Rhabditis sp. 71.9%; P. redivivus 92.7%; and Butlerius sp. 100% (p≤0.05). The activity showed by C. rosea against the H. contortus can be crucial for further studies focused to the biological control of sheep haemonchosis, although the environmental impact against beneficial nematodes should be evaluated.
Photocatalytic Efficiency of TiO2-Biomass Loaded Mixture for Wastewater Treatment
Authors: Kanakaraju Devagi , Wong Soon Pang
Published: March 2018

The objective of this study was to assess the efficiency of a novel TiO2/modified sago bark (TiO2/MSB) mixture for the degradation of sago wastewater effluent by employing response surface methodology (RSM) using chemical oxygen demand (COD) removal as the target parameter. The highest COD removal of 64.92% was obtained using TiO2/MSB mixture sample prepared by combining 0.2 g/L TiO2and 1 w/w% MSB. Given that the highest removal was produced using this mixture sample, further optimisation of sago wastewater treatment was conducted by varying the independent variables, namely, dosage and contact time. Under this optimum condition, 0.10 g of 0.2 g/L TiO2/1% MSB had successfully reduced 52.83% COD in 120 min. Surface morphology, functional groups, and elemental analysis supported observations of the ability of TiO2/MSB mixture to remove COD. Additionally, aeration had further improved COD removal by 11%. The regression value (R2>0.99) of the model indicated a high degree of correlation between the evaluated parameters. These results proved the feasibility of TiO2photocatalysis as an appealing alternative protocol for sago wastewater treatment and solid waste from the industry can be utilised for wastewater degradation.
Screening of Serum Protein Markers for Avascular Osteonecrosis of Femoral Head Differentially Expressed after Treatment with Yuanshi Shengmai Chenggu Tablets
Authors: Deng Peng , Zeng Jianchun , Li Jie , Feng Wenjun , Chen Jinlun , Zeng Yirong
Published: March 2018

Avascular necrosis of the femoral head (ANFH) is an a frequently occurring orthopaedic disease with high morbidity. Traditional Chinese Medicine (TCM) Yuanshi Shengmai Chenggu Tablet is a valid prescription for treating steroid-induced femoral head necrosis. However, there are rare investigations about the serum protein marker expression after the acting of drugs on hormone and TCM. In the present study, we aimed to systematically discover and validate the serum biomarkers expression difference in patients with steroid-induced avascular necrosis of femoral head (SANFH) after taking Yuanshi Shengmai Chenggu Tablets (SANFH-TCM), so as to reveal the action mechanism of TCM from the molecular level by using isobaric tags for relative and absolute quantification (iTRAQ) with multiple reaction monitoring quantification. Significant differences in fibrinogen alpha, fibrinogen beta, fibrinogen gamma, fibronectin, C-reactive protein, apolipoprotein A, apolipoprotein D, and apolipoprotein E were found among SANFH, SANFH-TCM, and healthy controls. Therefore, our study proposes potential biomarkers for SANFH diagnosis and for the prognosis of femoral head necrosis after Traditional Chinese Medicine treatment.
Superfield Approach to Nilpotency and Absolute Anticommutativity of Conserved Charges: 2D Non-Abelian 1-Form Gauge Theory
Authors: Kumar S. , Chauhan B. , Malik R. P.
Published: March 2018

We exploit the theoretical strength of augmented version of superfield approach (AVSA) to Becchi-Rouet-Stora-Tyutin (BRST) formalism to express the nilpotency and absolute anticommutativity properties of the (anti-)BRST and (anti-)co-BRST conserved charges for the two (1+1)-dimensional (2D) non-Abelian 1-form gauge theory (without any interaction with matter fields) in the language of superspace variables, their derivatives, and suitable superfields. In the proof of absolute anticommutativity property, we invoke the strength of Curci-Ferrari (CF) condition for the (anti-)BRST charges. No such outside condition/restriction is required in the proof of absolute anticommutativity of the (anti-)co-BRST conserved charges. The latter observation (as well as other observations) connected with (anti-)co-BRST symmetries and corresponding conserved charges are novel results of our present investigation. We also discuss the (anti-)BRST and (anti-)co-BRST symmetry invariance of the appropriate Lagrangian densities within the framework of AVSA. In addition, we dwell a bit on the derivation of the above fermionic (nilpotent) symmetries by applying the AVSA to BRST formalism, where only the (anti)chiral superfields are used.
Statistical Validation for Clinical Measures: Repeatability and Agreement of Kinect™-Based Software
Authors: Lopez Natalia , Perez Elisa , Tello Emanuel , Rodrigo Alejandro , Valentinuzzi Max E.
Published: March 2018

Background. The rehabilitation process is a fundamental stage for recovery of people’s capabilities. However, the evaluation of the process is performed by physiatrists and medical doctors, mostly based on their observations, that is, a subjective appreciation of the patient’s evolution. This paper proposes a tracking platform of the movement made by an individual’s upper limb using Kinect sensor(s) to be applied for the patient during the rehabilitation process. The main contribution is the development of quantifying software and the statistical validation of its performance, repeatability, and clinical use in the rehabilitation process. Methods. The software determines joint angles and upper limb trajectories for the construction of a specific rehabilitation protocol and quantifies the treatment evolution. In turn, the information is presented via a graphical interface that allows the recording, storage, and report of the patient’s data. For clinical purposes, the software information is statistically validated with three different methodologies, comparing the measures with a goniometer in terms of agreement and repeatability. Results. The agreement of joint angles measured with the proposed software and goniometer is evaluated with Bland-Altman plots; all measurements fell well within the limits of agreement, meaning interchangeability of both techniques. Additionally, the results of Bland-Altman analysis of repeatability show 95% confidence. Finally, the physiotherapists’ qualitative assessment shows encouraging results for the clinical use. Conclusion. The main conclusion is that the software is capable of offering a clinical history of the patient and is useful for quantification of the rehabilitation success. The simplicity, low cost, and visualization possibilities enhance the use of the software Kinect for rehabilitation and other applications, and the expert’s opinion endorses the choice of our approach for clinical practice. Comparison of the new measurement technique with established goniometric methods determines that the proposed software agrees sufficiently to be used interchangeably.
Effect of Icariin on Tibial Dyschondroplasia Incidence and Tibial Characteristics by Regulating P2RX7 in Chickens
Authors: Zhang Hui , Mehmood Khalid , Jiang Xiong , Yao Wangyuan , Iqbal Mujahid , Li Kun , Tong Xiaole , Wang Lei , Wang Meng , Zhang Lihong , Nabi Fazul , Rehman Mujeeb Ur , Li Jiakui
Published: March 2018

Tibial dyschondroplasia (TD) is a disease of rapid growing chickens that occurs in many avian species; it is characterized by nonvascular and nonmineralized growth plates, along with tibia bone deformation and lameness. Icariin is widely used to treat bone diseases in humans, but no report is available regarding the effectiveness of icariin against avian TD. Therefore, this study was designed to determine its effect against TD. For this purpose, a total of 180 broiler chicks were distributed into three groups including control, TD, and icariin group. Control group was given a standard normal diet, while TD and icariin groups received normal standard diet containing 50 mg/kg thiram to induce TD from days 3 to 7 after hatch. After the induction of TD, the chicks of icariin group were fed with standard normal diet by adding 10 mg/kg icariin in water. Then morphological and production parameters analysis of tibial bone indicators, physiological index changes, and gene expression were examined. The results showed that icariin administration not only decreased the mortality but also mitigated the lameness and promoted the angiogenesis, which diminished the TD lesion and significantly increased the expression of P2RX7 (P<0.05) in TD affected thiram induced chicks. In conclusion, present findings suggest that icariin has a significant role in promoting the recovery of chicken growth plates affected by TD via regulating the P2RX7. Our findings reveal a new target for clinical treatment and prevention of TD in broiler chickens.
Numerical Analysis of a Real Photovoltaic Module with Various Parameters
Authors: Nituca Costica , Chiriac Gabriel , Cuciureanu Dumitru , Zhang Guoqiang , Han Dong , Plesca Adrian
Published: March 2018

This article presents a real photovoltaic module with modeling and simulations starting from the model of a photovoltaic (PV) cell. I-V, P-V, and P-I characteristics are simulated for different solar irradiation, temperatures, series resistances, and parallel resistances. For a real photovoltaic module (ALTIUS Module AFP-235W) there are estimated series and parallel resistances for which the energetical performances of the module have optimal values for a solar radiation of 1000 W/m2 and a temperature of the environment of 25°C. Temperature influence over the PV module performances is analyzed by using a thermal model of the ALTIUS Module AFP-235W using the finite element method. A temperature variation on the surface of the PV module is starting from a low value 40.15°C to a high value of 52.07°C. Current and power estimation are within the errors from 1.55% to about 4.3%. Experimental data are measured for the photovoltaic ALTIUS Module AFP-235W for an entire daylight.
In Situ Preservation Fraction of Parathyroid Gland in Thyroidectomy: A Cohort Retrospective Study
Authors: Luo Han , Zhao Wanjun , Yang Hongliu , Su Anping , Wang Bin , Zhu Jingqiang
Published: March 2018

Background and Objectives. Parathyroid failure is the most common symptom after thyroidectomy. To prevent it, a gland was preserved in situ or an ischemic one was autotransplanted. This study explored the relationship between in situ preservation of the parathyroid gland and gland failure. Methods. Consecutive patients who underwent initial total thyroidectomy were enrolled retrospectively in a prospectively maintained database. Patients were divided into groups by parathyroid gland remaining in situ fraction (PGRIF) (PGRIF = number of in situ glands/(total number of identified glands − number of glands in specimen). Patients were graded by tertiles and followed at least one year after surgery. Results. 559 patients were included. PGRIF is significantly inversely associated with transient hypoparathyroidism, protracted hypoparathyroidism, and postoperative hypocalcemia. PGRIF was identified as an independent risk factor for transient hypoparathyroidism, protracted hypoparathyroidism, and postoperative hypocalcemia (OR=0.177, 0.190, and 0.330, resp.). Autotransplantation of parathyroid gland would not affect the calcium level in the long term. Conclusion. In situ preservation of parathyroid gland is crucial for parathyroid function. Less preserved is the independent risk factor for postoperative hypoparathyroidism and hypocalcemia, resulting in a worse function of parathyroid gland in the long term.
Peripheral Lymph Node Excisional Biopsy: Yield, Relevance, and Outcomes in a Remote Surgical Setup
Authors: Shrestha Ashish Lal , Shrestha Pradita
Published: March 2018

Objective. To study the patient profile for symptomatic peripheral lymphadenopathy in terms of histopathological findings and demography and evaluate the yield, relevance, and outcomes of peripheral lymph node biopsy (PLNB) as a diagnostic step in a remote setup in the absence of less invasive options like fine-needle aspiration cytology (FNAC) or ultrasonogram- (USG-) guided FNAC.Methods. A retrospective review of patients undergoing PLNB between 1 May 2011 and 30 April 2013 was done. Demographics, histopathological reports, and outcomes were studied.Results. Of 132 patients, 51 (38.63%) were male and 81 (61.36%) were female. There were 48 (36.3%) patients in the age group less than 16 years, and 84 (63.6%) were beyond 16 years. The commonest site of biopsy was the neck in 114 (86.36%) patients. The histopathological diagnosis was tuberculosis (TB) in 60 (45.45%) patients, reactive lymphadenitis in 29 (21.9%), nonspecific granuloma in 18 (13.6%), lymphoma in 7 (5.3%), acute lymphadenitis in 7 (5.3%), metastatic secondary in 3 (2.2%), and other benign causes in 8 (6.06%).Conclusions. PLNB is a procedure with good diagnostic yield in evaluation of peripheral lymphadenopathy. Its relevance is appreciable in a remote setup where less invasive options are unavailable. Its simplicity and lack of mortality/significant morbidity make it a valid option in rural surgical practice.
A Study of the Effect of the Front-End Styling of Sport Utility Vehicles on Pedestrian Head Injuries
Authors: Zhang Guanjun , Qin Qin , Chen Zheng , Bai Zhonghao , Cao Libo
Published: March 2018

Background. The number of sport utility vehicles (SUVs) on China market is continuously increasing. It is necessary to investigate the relationships between the front-end styling features of SUVs and head injuries at the styling design stage for improving the pedestrian protection performance and product development efficiency. Methods. Styling feature parameters were extracted from the SUV side contour line. And simplified finite element models were established based on the 78 SUV side contour lines. Pedestrian headform impact simulations were performed and validated. The head injury criterion of 15 ms (HIC15) at four wrap-around distances was obtained. A multiple linear regression analysis method was employed to describe the relationships between the styling feature parameters and the HIC15 at each impact point. Results. The relationship between the selected styling features and the HIC15 showed reasonable correlations, and the regression models and the selected independent variables showed statistical significance. Conclusions. The regression equations obtained by multiple linear regression can be used to assess the performance of SUV styling in protecting pedestrians’ heads and provide styling designers with technical guidance regarding their artistic creations.
Synthesis, Characterization, and Antimicrobial Studies of Novel Series of 2,4-Bis(hydrazino)-6-substituted-1,3,5-triazine and Their Schiff Base Derivatives
Authors: Al-Rasheed Hessa H. , Sholkamy Essam N. , Al Alshaikh Monirah , Siddiqui Mohammed R. H. , Al-Obaidi Ahmed S. , El-Faham Ayman
Published: March 2018

The present work represents the synthesis, characterization, and antimicrobial studies of novel series of 2,4-bis(hydrazino)-6-substituted-1,3,5-triazine and their Schiff base derivatives. IR, NMR (H1 and C13), elemental analysis, and LC-MS characterized the prepared compounds. The biological activity of the target products was evaluated as well. Twenty-two of the prepared compounds were selected according to their solubility in aqueous DMSO. Only eight compounds showed good activity against the selected pathogenic bacteria and did not show antagonistic effect against fungus Candida albicans. Two compounds 4k and 5g have wide-range effect presently in Gram-positive and Gram-negative bacteria while other compounds (4f, 4i, 4m, 5d, 6i, and 6h) showed specific effect against the Gram-negative or Gram-positive bacteria. The minimum inhibitory concentration (MIC, μg/mL) of 4f, 4i, 4k, and 6h compounds against Streptococcus mutans was 62.5 μg/mL, 100 μg/mL, 31.25 μg/mL, and 31.25 μg/mL, respectively. The MIC of 4m, 4k, 5d, 5g, and 6h compounds against Staphylococcus aureus was 62.5 μg/mL, 31.25 μg/mL, 31.25 μg/mL, 100 μg/mL, and 62.5 μg/mL, respectively. The MIC of 4k, 5g, and 6i compounds against Salmonella typhimurium was 31.25 μg/mL, 100 μg/mL, and 62.5 μg/mL, respectively. The MIC of 6i compound against Escherichia coli was 62.5 μg/mL.
Double Barrel Nasal Trumpets to Prevent Upper Airway Obstruction after Nasal and Non-Nasal Surgery
Authors: Camacho Macario , Wei Justin M. , Reckley Lauren K. , Song Sungjin A.
Published: March 2018

Objectives. During anesthesia emergence, patients are extubated and the upper airway can become vulnerable to obstruction. Nasal trumpets can help prevent obstruction. However, we have found no manuscript describing how to place nasal trumpets after nasal surgery (septoplasties or septorhinoplasties), likely because (1) the lack of space with nasal splints in place and (2) surgeons may fear that removing the trumpets could displace the splints. The objective of this manuscript is to describe how to place nasal trumpets even with nasal splints in place.Materials and Methods. The authors describe techniques (Double Barrel Technique and Modified Double Barrel Technique) that were developed over three years ago and have been used in patients with obstructive sleep apnea (OSA) and other patients who had collapsible or narrow upper airways (i.e., morbidly obese patients).Results. The technique described in the manuscript provides a method for placing one long and one short nasal trumpet in a manner that helps prevent postoperative upper airway obstruction. The modified version describes a method for placing nasal trumpets even when there are nasal splints in place. Over one-hundred patients have had nasal trumpets placed without postoperative oxygen desaturations.Conclusions. The Double Barrel Technique allows for a safe emergence from anesthesia in patients predisposed to upper airway obstruction (such as in obstructive sleep apnea and morbidly obese patients). To our knowledge, the Modified Double Barrel Technique is the first description for the use of nasal trumpets in patients who had nasal surgery and who have nasal splints in place.
Performance Evaluation of Two ANFIS Models for Predicting Water Quality Index of River Satluj (India)
Authors: Tiwari Sharad , Babbar Richa , Kaur Gagandeep
Published: March 2018

Water quality index is the most convenient way of communicating water quality status of water bodies, but its evaluation requires subjectivity in terms of user involvement and dealing with uncertainty. Recently, artificial intelligence algorithms that are appropriate for nonlinear forecasting and also dealing with uncertainties have been applied to various domains of water quality forecasting. This paper focuses on development of a data‐driven adaptive neurofuzzy system for the water quality index using a real data set obtained from eight different monitoring stations across River Satluj in northern India. Novelty in the paper lies in the estimation of water quality index using two different clustering techniques: fuzzy C‐means and subtractive clustering‐based ANFIS and assessing their predictive accuracy. Each model was used to train, validate, and test the index that was obtained from seven water quality parameters including pH, conductivity, chlorides, nitrates, ammonia, and fecal coliforms. The models were evaluated on the basis of statistical performance criteria. Based on the evaluations, it was found that the SC‐ANFIS method gave more accurate result as compared to the FCM‐ANFIS. The tested model, SC‐ANFIS model, was further used to identify those sensitive parameters across various monitoring stations that were capable of causing change in the existing water quality index value.
Factors Associated with Participation in Pulmonary Tuberculosis Screening Using Chest X-Ray among Diabetes Mellitus Type II Patients in Denpasar, Bali, Indonesia
Authors: Putra I Gusti Ngurah Edi , Astuti Putu Ayu Swandewi , Suarjana I Ketut , Mulyawan Ketut Hari , Duana I Made Kerta , Kurniasari Ni Made Dian , Putra I Wayan Gede Artawan Eka
Published: March 2018

Diabetes mellitus (DM) increases the risk of developing pulmonary tuberculosis (TB) disease. Therefore, pulmonary TB screening among DM patients is essential. This study aimed to identify factors associated with participation of DM type II patients in pulmonary TB screening using chest X-ray. This was a cross-sectional analytic study and was part of TB-DM screening study in Denpasar, Bali, Indonesia. The sample consisted of 365 DM type II patients selected by quota sampling among DM type II patients joining the screening program from January until March 2016 in 11 public health centres in Denpasar. Data were collected via structured interviews. The contributing factors were determined by modified Poisson regression test for cross-sectional data. From the findings, less than half (45.48%) of DM type II patients participated in chest X-ray examination for TB. Factors associated with participation in pulmonary TB screening were having a higher educational level [APR = 1.34, 95% CI (1.07–1.67)], having family member who developed pulmonary TB disease [APR = 1.47, 95% CI (1.12–1.93)], the travel time to referral hospital for screening being ≤ 15 minutes [APR = 1.6, 95% CI (1.26–2.03)], having health insurance [APR = 2.69, 95% CI (1.10–6.56)], and receiving good support from health provider [APR = 1.35, 95% CI (1.06–1.70)]. Therefore, training for health provider on providing counselling, involvement of family members in screening process, and improving the health insurance coverage and referral system are worth considering.
Improved Risk Stratification by Circulating Tumor Cell Counts in Pancreatic Cancer
Authors: Effenberger Katharina E. , Schroeder Cornelia , Hanssen Annkathrin , Wolter Stefan , Eulenburg Christine , Tachezy Michael , Gebauer Florian , Izbicki Jacob R. , Pantel Klaus , Bockhorn Maximilian
Published: March 2018

Purpose: Pancreatic cancer is one of the most devastating diseases with a 5-year survival rate of 3% to 5%. Here, we investigated whether circulating tumor cells (CTC) may predict metastatic spread and survival in pancreatic cancer patients.

Experimental Design: In a prospective study, we enrolled 69 pancreatic cancer patients. In peripheral blood, CTCs were identified by MACS enrichment (anti-cytokeratin/anti-EpCam) and subsequent automated analysis after combined anti-cytokeratin/anti-CD45/DAPI staining. CTC results were correlated to established clinicopathologic risk factors, detection of disseminated tumor cells (DTC) in bone marrow, and clinical outcome (follow-up time: 48 months).

Results: Median patient survival was 11 months (0–48 months). Thirty-eight patients were male and 31 were female, and the majority received gemcitabine (58/69). CTCs were present in 23 of 69 patients (33.3%) ranging from 1 to 19 cells (17 with >1 CTC). Although clinicopathologic parameters and DTC status did not correlate with CTC incidence, progression-free survival (PFS) and overall survival (OS) were significantly reduced in CTC-positive patients in univariate (P = 0.009, PFS; P = 0.030, OS, both log rank) and multivariate analysis [HR = 4.543; confidence interval (CI), 1.549–13.329; P = 0.006, PFS; HR = 2.093; CI, 1.081–4.050; P = 0.028, OS, both Cox regression). Also within patients receiving chemotherapy, PFS was significantly reduced in CTC-positive patients in univariate (P = 0.013) and multivariate (HR = 4.203; CI, 1.416–12.471; P = 0.010) analysis.

Conclusions: CTCs affect the outcome of patients with pancreatic cancer independent from other risk factors, including patients receiving (adjuvant) cytotoxic therapy. CTC stratification may allow a better upfront identification of patients with a longer lifespan who might profit from new adjuvant therapies. Clin Cancer Res; 24(12); 2844–50. ©2018 AACR.
Vitamin B12 in Association with Antipsychotic Drugs Can Modulate the Expression of Pro-/Anti-Inflammatory Cytokines in Alzheimer Disease Patients
Authors: Vakilian Alireza , Razavi-Nasab Seyed Moein , Ravari Ali , Mirzaei Tayebeh , Moghadam-Ahmadi Amir , Jalali Nazanin , Bahramabadi Reza , Rezayati Mohammadtaghi , Yazdanpanah-Ravari Amin , Bahmaniar Farhad , Bagheri Mohammad Reza , Sheikh Fathollahi Mahmood , Asadikaram Gholamreza , Kazemi Arababadi Mohammad
Published: March 2018

Introduction: Patients with Alzheimer disease (AD) suffer from psychotic symptoms including pain. The current antipsychotic drugs confer limited effectiveness, and hence new strategies are being designed to decrease pain in order to increase antipsychological effectiveness. Vitamin B12 is a safe supplementary drug to decrease pain. Additionally, cytokines participate in the pathogenesis of immune-related diseases such as AD. Thus, the main aim of this clinical trial study was to determine the effects of treatment with risperidone and quetiapine, as antipsychotic drugs, with and without vitamin B12 on the psychotic symptoms of AD patients and the expression of IL-6, IL-8, tumor growth factor (TGF)-β, tumor necrosis factor (TNF)-α, and endothelin (ET)-1). Material and Methods: Serum levels of IL-6, IL-8, TGF-β, TNF-α, and ET-1 were evaluated in the following groups: healthy controls, nonpsychotic AD patients, psychotic AD patients, psychotic AD patients under treatment with risperidone, psychotic AD patients under treatment with risperidone plus vitamin B12, psychotic AD patients under treatment with quetiapine, and psychotic AD patients under treatment with quetiapine plus vitamin B12. Results: Treatment with antipsychotic drugs plus vitamin B12 led to a decreased expression of IL-8 and TNF-α and an increased expression of TGF-β. Vitamin B12 in association with quetiapine reduced the pain in psychotic AD patients. Discussion: Proinflammatory cytokines play important roles in the pathogenesis of psychosis in AD patients. Antipsychotic drugs plus vitamin B12 can reduce and induce the expression of proinflammatory and anti-inflammatory cytokines to improve psychotic symptoms in AD patients.
Epidemiology of Myasthenia Gravis in Slovakia in the Years 1977–2015
Authors: Martinka Ivan , Fulova Miriam , Spalekova Margita , Spalek Peter
Published: March 2018

Purpose: The objective of the study was to evaluate changes of epidemiological parameters in patients with myasthenia gravis (MG) in Slovakia during the period 1977–2015. Methods: Data from medical records of MG patients registered in Slovakia were analyzed. Epidemiological rates (incidence, prevalence, mortality) were assessed for several periods to identify changes and drifts over the period of study. Results: Out of 2,074 patients, 892 were males (43.0%) and 1,193 were females (57.0%). The thymoma associated MG (TAMG) was diagnosed in 123 patients (5.9%). The mean age at onset shifted from 35.8 years in 1977–1989 to 60.0 years in ­2010–2015. The crude incidence increased from 0.36/100,000 in 1977–1989 to 1.74/100,000 in 2010–2015. The average annual incidence of TAMG was 0.05/100,000. Maximum increase of MG incidence was registered among the elderly (≥50 years), particularly in the group of patients in the age range 70–79 years (0.34/100,000 in 1977–1989→7.10/100,000 in 2010–2015) and 80–89 years (0.00/100,000 in 1977–1989→5.31/100,000 in 2010–2015). The crude MG prevalence on December 31, 2015 was 24.75/100,000. The average MG mortality was 0.27/100,000. Conclusion: The age at onset and incidence increased significantly over the study period due to marked increase of MG incidence in elderly, particularly over 70 years. Possible reasons for this trend are discussed.
Heparin from bovine intestinal mucosa: Glycans with multiple sulfation patterns and anticoagulant effects
Authors: Tovar Ana M. F. , Capillé Nina V. M. , Santos Gustavo R. C. , Vairo Bruno C. , Oliveira Stephan-Nicollas M. C. G. , Fonseca Roberto J. C. , Mourão Paulo A. S.
Published: March 2018

Pharmaceutical grade heparins from porcine intestine and bovine lung consist mainly of repeating tri-sulfated units, of the disaccharide →4-α-IdoA2S-1 →4-α-GlcNS6S-1 →. Heparin preparations from bovine intestine, in contrast, are more heterogeneous. Nuclear magnetic resonance (NMR) and disaccharide analysis after heparinase digestions show that heparin from bovine intestine contains α-glucosamine with significant substitutive variations: 64% are 6-O-sulfated and N-sulfated, as in porcine intestinal heparin while 36% are 6-desulfated. Desulfated α-iduronic acid units are contained in slightly lower proportions in bovine than in porcine heparin. NMR data also indicate N-, 3-and 6-trisulfated a-glucosamine (lower proportions) and α-GlcNS-1→4-α-GlcA and α-IdoA2S-1→4-α-GlcNAc (higher amounts) in bovine than in porcine heparin. Porcine and bovine heparins can be fractionated by anion exchange chromatography into three fractions containing different substitutions on the a-glucosamine units. Each individual fraction shows close disaccharide composition and anticoagulant activity, regardless of their origin (bovine or porcine intestine). However, these two heparins differ markedly in the proportions of the three fractions. Interestingly, fractions with the typical he-parin disaccharides of porcine intestine are present in bovine intestinal heparin. These fractions contain high in vitro anticoagulant activity, reduced antithrombotic effect and high bleeding tendency. These observations indicate that the prediction of haemostatic effects of heparin preparations cannot rely exclusively on structural analysis and anticoagulant assays in vitro. Minor structural components may account for variations on in vivo effects. In conclusion, we suggest that pharmaceutical grade bovine intestinal heparin, even after purification procedures, is not an equivalent drug to porcine intestinal heparin.
Pleione jinhuana (Arethuseae; Epidendroideae; Orchidaceae), a new species from China based on morphological and DNA evidence
Authors: JIANG MING-TAO , WU SHA-SHA , LIU ZHONG-JIAN , LAN SI-REN
Published: March 2018

Pleione jinhuana, a new orchid from China, is illustrated and described based on morphological and phylogenetic evidence. Detailed morphological comparisons between the new species and other members of Pleione indicate that P. jinhuana is similar to P. bulbocodioides and P. formosana but differs in having a lip with an erose apical margin and lateral lobes not enfolding the column. To explore the phylogenetic position of this species, nuclear ribosomal ITS and plastid DNA (matK exon, trnT-trnL spacer, trnL exon and trnL-trnF spacer) were sequenced for P. jinhuana and individuals of P. formosana collected in Taiwan, Fujian and Zhejiang Provinces. The morphological and molecular evidence support the hypothesis that P. jinhuana is a new species.
Nematodes from galls on Myrtaceae. XI. Descriptions of five new species of Fergusobia from Australia
Authors: DAVIES KERRIE A. , YE WEIMIN , TAYLOR GARY S. , SCHEFFER SONJA , BARTHOLOMAEUS F. , GIBLIN-DAVIS ROBIN M.
Published: March 2018

Five new species of Fergusobia are described. Fergusobia janetae Davies n. sp. is characterized by the combination of an arcuate to straight, spindle-shaped parthenogenetic female with a relatively small oesophageal gland, an extensile uterus and a conoid tail, an arcuate infective female with a bluntly rounded tail tip, and a C-shaped male with an arcuate to angular spicule and bursa arising at more than 90% of body length. Fergusobia robustae Davies n. sp. is characterized by the combination of a small, C-shaped parthenogenetic female with a short cylindroid tail with a broadly rounded tip; an arcuate to C-shaped, relatively broad, infective female with a tail tip that is almost hemispherical; and an arcuate male with a strong angular spicule and a bursa arising at 30–40% of body length. Fergusobia pruinosae Davies n. sp. is characterized by the combination of a medium sized, open C-shaped, parthenogenetic female in which the cuticle does not swell upon fixation, with a strongly sclerotised stylet, with a more or less narrowly conoid tail with a bluntly or broadly rounded tip; infective female that is arcuate to J-shaped with a notched tail tip; and open C-shaped males with stout, angular spicules and bursa arising near the secretory/excretory pore. Fergusobia pauciflorae Davies n. sp. is characterized by the combination of a medium sized, arcuate, parthenogenetic female with a small stylet, and a conoid tail with a bluntly rounded tip; a small, open C to J-shaped infective female with a broadly rounded to almost hemispherical tail tip; and straight to arcuate males with stout, angular spicules and bursa at ~70% of body length anterior to the cloaca. Fergusobia obliquae Davies n. sp. has a relatively broad, arcuate, parthenogenetic female having a small but strongly sclerotised stylet, and a short conoid tail with a bluntly rounded tip; an arcuate, infective female with an almost hemispherical tail tip; and C-shaped males with arcuate to angular (not heavily sclerotised) spicules and bursa arising 50–80% of body length anterior to tail. Sequencing of the 18S and 28S rDNA domains for F. janetae n. sp. and of mitochondrial DNA cytochrome oxidase subunit I (mtCOI) for F. pauciflorae n. sp. confirm that they are distinct species.
On the Coupling between Barotropic and Baroclinic Modes of Extratropical Atmospheric Variability
Authors: Boljka Lina , Shepherd Theodore G. , Blackburn Michael
Published: March 2018

The baroclinic and barotropic components of atmospheric dynamics are usually viewed as interlinked through the baroclinic life cycle, with baroclinic growth of eddies connected to heat fluxes, barotropic decay connected to momentum fluxes, and the two eddy fluxes connected through the Eliassen–Palm wave activity. However, recent observational studies have suggested that these two components of the dynamics are largely decoupled in their variability, with variations in the zonal mean flow associated mainly with the momentum fluxes, variations in the baroclinic wave activity associated mainly with the heat fluxes, and essentially no correlation between the two. These relationships are examined in a dry dynamical core model under different configurations and in Southern Hemisphere observations, considering different frequency bands to account for the different time scales of atmospheric variability. It is shown that at intermediate periods longer than 10 days, the decoupling of the baroclinic and barotropic modes of variability can indeed occur as the eddy kinetic energy at those time scales is only affected by the heat fluxes and not the momentum fluxes. The baroclinic variability includes the oscillator model with periods of 20–30 days. At both the synoptic time scale and the quasi-steady limit, the baroclinic and barotropic modes of variability are linked, consistent with baroclinic life cycles and the positive baroclinic feedback mechanism, respectively. In the quasi-steady limit, the pulsating modes of variability and their correlations depend sensitively on the model climatology.