bluuebunny/crossref_metadata_2025 huggingface dataset:
Le Roman de Flamenca, un désir d’amitié
Authors: Pilloux Adélaïde
Published: February 2025

Le Roman de Flamenca, œuvre composée en occitan dans la première moitié du xiiie siècle, est habituellement lu comme un récit d’amour courtois : enfermée et surveillée par Archambaut, son époux jaloux, Flamenca fait la rencontre de Guilhem, un noble chevalier à l’église. Les futurs amants profitent des quelques instants partagés lors de la messe pour se murmurer un mot chaque semaine. Ce dispositif de déclaration fragmentée et retardée a fait l’objet de nombreuses analyses. Cependant, rares sont celles qui soulignent la participation des deux demoiselles de Flamenca – Marguerite et Alice – ou l’importance plus générale de la relation entre les trois dames. Cet article propose de relire le Roman de Flamenca au prisme de l’amitié féminine pour souligner la singularité de cette relation ainsi que sa fonction au sein du récit.
Où sont les sorcières de la Lune Rouge ? Le vernis médiéval d’un féminisme menstruel
Authors: Pla Nadia
Published: February 2025

La vague féministe de ces vingt dernières années accorde une importance particulière au corps féminin, et notamment à la menstruation. Des autrices de pages internet grand public développent un intérêt nouveau pour ce sujet et tentent de lui donner une dimension historique, avec le risque d’une vision parfois idéalisée et caricaturale de l’attitude envers la menstruation à des époques anciennes, parmi lesquelles le Moyen Âge est privilégié. Cet article analyse quelques cas de fausses affirmations circulant sur des pages de ce type, par exemple celles qui concernent la « lune rouge ». Il remet ces affirmations en perspective, en les replaçant dans le cadre des mouvances de l’écoféminisme et du féminin sacré. Il les confronte à ce que les sources médiévales disent réellement du lien de la menstruation avec le cycle lunaire, les âges de la vie, ou la sorcellerie.
Federica Buttò, Le « Tristan en prose » du ms. BnF français 756 : texte et étude
Authors: Buttò Federica
Published: February 2025

Seize ans après la dernière publication d’un volume du Tristan en prose (2007), ce travail vient compléter cette édition s’appuyant sur le ms. Paris, Bibliothèque nationale de France, français 757, en rendant accessible la première moitié du roman contenue dans le codex Paris, Bibliothèque nationale de France, français 756. Cette thèse, en plus de mettre à disposition le texte d’un témoin important, met en évidence de nouveaux liens entre certains codices de la tradition manuscrite, apporte des informations supplémentaires sur les témoins utilisés pour la collation et des éclaircissements sur l’histoire du manuscrit Paris, Bibliothèque nationale de France, français 756 (enlumineurs, propriétaires, clients, modalités et temps de fabrication, etc.) et contribue aux travaux linguistiques sur le franco-italien et sur le « français de Naples ». Tout cela a été possible grâce à une approche interdisciplinaire croisant paléographie, codicologie, histoire, philologie, littérature et linguistique. Ainsi la thèse ouvre de nouvelles pistes de recherche sur la tradition manuscrite du Tristan en prose, qui reste un champ d’investigation ouvert, prometteur, qui témoigne d’une réception arthurienne vivante en Italie.
Clara de Raigniac, Un récit de guerre médiéval : les Chroniques de Froissart en leurs manuscrits
Authors: Raigniac Clara de
Published: February 2025

Cette thèse a pour objectif d’étudier le récit de guerre médiéval en l’inscrivant dans le champ des war studies. Plutôt que nous limiter au texte, nous choisissons de considérer le codex dans son ensemble, depuis sa production par l’auteur et les artisans du livre à sa réception par un public. Les Chroniques de Froissart nous servent de laboratoire. La première partie de notre travail entend circonscrire le phénomène : en s’inspirant de modèles épiques, romanesques et historiographiques antérieurs, Froissart et les artisans du livre transforment la réalité complexe de la guerre de Cent Ans en mots et motifs réguliers qui permettent de tracer une poétique du récit de guerre. La deuxième partie de notre réflexion envisage un autre cadre pour le récit de guerre, celui de l’histoire. En revenant à la définition selon laquelle « Historia est narratio rei gestae », nous nous penchons sur la temporalité du manuscrit de guerre et sur l’ethos de Froissart. Les seuils des Chroniques, sans cesse adaptés par les libraires, constituent un niveau d’adaptation supplémentaire de la matière militaire au canevas historique. Notre réflexion se tourne enfin vers la manière dont les topoï du récit de guerre sont actualisés pour les lecteurs des xive-xve siècles. Quelle idéologie guerrière Froissart développe-t-il ? Comment a-t-elle été reçue ? Si nous analysons les marques qui, dans les manuscrits, témoignent de lectures réelles du récit de guerre, les preuves restent ténues dans ces codices de luxe : c’est finalement à la lumière d’un bref exercice de fiction que nous tentons de montrer comment la lecture aurale faisait des Chroniques de Froissart un passe-temps édifiant partagé par la noblesse.
Julien Florent, Anseïs de Carthage – Édition critique du manuscrit BnF, fr. 793
Authors: Florent Julien
Published: February 2025

Notre thèse propose une nouvelle édition critique de la chanson de geste Anseïs de Carthage, datée du milieu du xiiie siècle, à partir du manuscrit Paris, Bibliothèque nationale de France, fr. 793. Le texte d’Anseïs von Karthago, publié par Johann Alton en 1892, ne répondait plus aux normes scientifiques actuelles et une nouvelle édition était donc nécessaire. Notre travail suit les principes d’édition des manuscrits médiévaux préconisés par l’École nationale des chartes. Nous avons relevé toutes les variantes en nous appuyant sur l’ensemble de la tradition manuscrite : celle‑ci comporte trois manuscrits complets (Paris, Bibliothèque nationale de France, fr. 1598 et 12548 et Lyon, Bibliothèque municipale, P.A. 59), le manuscrit de Durham (Cathedral Library, V. II. 17) amputé de son début et ignoré par J. Alton, et quelques courts fragments. L’introduction grammaticale étudie la langue de la copie, du point de vue graphique et phonétique, ainsi que dans ses principales caractéristiques morphologiques, syntaxiques et métriques. Le manuscrit de base [A] présente deux mains successives, dont les habitudes propres à chaque copiste sont signalées. Il se distingue par son caractère régional, tant par la présence de traits phonétiques et morphologiques propres à la scripta picarde que par des hapax ou mots régionaux. L’édition est accompagnée d’un glossaire qui vise l’exhaustivité et d’un index des noms propres qui s’efforce de les identifier. À partir de ces éléments (notamment des toponymes), des hypothèses sont proposées sur la localisation de l’œuvre (Hainaut, Cambrésis, Vermandois) et ses possibles commanditaires. L’introduction littéraire propose plusieurs pistes de réflexion afin d’inscrire cette chanson de geste dans son contexte littéraire et historique : nous avons étudié comment ce texte est associé à d’autres œuvres selon les manuscrits considérés, l’intertextualité entre ces textes ainsi qu’avec les œuvres contemporaines : autres chansons de geste, lais bretons, romans antiques (notamment le Roman de Troie, car Anseïs de Carthage est caractérisé par une forte présence du mythe troyen), et l’hybridité générique entre roman et chanson de geste, trait que la chanson partage avec d’autres œuvres épiques à partir du xiiie siècle. Sont également étudiés la place de la religion, le traitement de l’espace et de la temporalité, ainsi que la question, centrale dans la chanson, du pouvoir et de sa transmission d’une génération héroïque à l’autre, montrant l’intérêt de l’auteur pour l’histoire de son temps.
Laure‑Anne Caraty, Réception et étude de la poésie lyrique médiévale de langue d'oc au siècle des Lumières : œuvres et manuscrits de La Curne de Sainte‑Palaye
Authors: Caraty Laure‑Anne
Published: February 2025

Notre thèse propose de combler une carence de l’histoire littéraire des troubadours en étudiant en détail les travaux de l’érudit parisien Sainte-Palaye, c’est-à-dire tous les manuscrits qui lui ont permis de travailler sur les troubadours. Notre objectif est de déterminer quel tournant représente le travail de cet érudit dans la réception des troubadours sous l’Ancien Régime, malgré l’oubli dans lequel est tombée sa contribution à cause de la publication de l’Histoire littéraire des troubadours rédigée par Millot (un abbé plus soucieux de préserver les mœurs des lecteurs que de faire connaître la fin’amor par les textes). Grâce aux annotations inscrites par Sainte-Palaye dans les marges de ses manuscrits, nous suivons pas à pas le labeur que ce dernier a mené dans plusieurs disciplines : philologie, linguistique, littérature et traduction. Nous essayons de déterminer quel regard cet académicien des Lumières non occitaniste porte sur cet ancien texte médiéval poétique.
Maxime Kamin, Représentations et poétiques du jeu dans la poésie française (langues d’oïl et d’oc) et latine du Moyen Âge (xiie-xiiie siècles)
Authors: Kamin Maxime
Published: February 2025

L’historiographie et la critique littéraire sont longtemps restées indifférentes à la place que tiennent les jeux dans la culture médiévale. La raison tient sans doute à la tradition de défiance dont s’entourent les activités ludiques, au mieux considérées par les autorités ecclésiastiques et civiles comme un passe-temps insignifiant et secondaire, au pire comme une vaine et coupable distraction susceptible de nuire à l’ordre social. Situant notre réflexion dans l’héritage des réflexions de Johan Huizinga et de Roger Caillois, nous avançons au contraire l’idée que le jeu, qui présente des points de rencontre avec le fonctionnement de la métaphore, se conçoit comme un outil d’analyse qui permet d’appréhender les codes et les valeurs qui structurent une société, d’orchestrer leur représentation et de leur assigner un sens. À cet égard, nous nous proposons d’interroger les différents contenus conceptuels dont la métaphore du jeu soutient l’expression dans les lettres médiévales. Optant pour une approche comparatiste, nous confrontons les usages variés de cette métaphore à travers un corpus d’œuvres qui mettent à l’honneur des auteurs qui se rattachent à différentes aires géographiques et culturelles. Notre thèse entend ainsi montrer comment le jeu concourt dans la poésie médiévale à l’expression et à la transmission d’un savoir d’ordre érotique, spirituel ou encore esthétique.
Marie-Christine Payne, « Par le femmenin gendre » : étude des personnages féminins dans le Roman de Perceforest
Authors: Payne Marie-Christine
Published: February 2025

Cette thèse se consacre aux personnages féminins du Roman de Perceforest. Le Perceforest est un récit qui se veut une genèse du lignage arthurien et qui fait même d’Alexandre le Grand l’ancêtre du roi Arthur. Pour se faire, l’auteur du Perceforest recourt à de nombreux motifs et procédés d’écriture, mais l’originalité du texte se trouve surtout dans la manière dont les personnages féminins sont envisagés. À cet effet, notre propos s’intéressera aux éléments qui constituent l’essence du personnage féminin dans le Perceforest. Quatre mouvements structurent notre étude durant laquelle nous prendrons appui sur les théories du personnage mais aussi sur des études portant sur la voix, la narration ou enfin sur les études de genre. Nous verrons que, loin d’être de simples prétextes narratifs ou des outils au service de l’accomplissement des chevaliers, les personnages féminins du Perceforest assurent non seulement la cohésion interne du récit mais permettent aussi de relire et de redynamiser d’autres textes et d’autres genres littéraires. Tout en conservant comme horizon principal l’avènement du lignage arthurien, le Perceforest relit les genres littéraires à travers le genre.
Marie-Antoinette Alamenciak, Revoir la théorie littéraire par le Moyen Âge. Le personnage dans les Quinze joies de mariage, les Cent Nouvelles nouvelles, les Arrêts d’Amour de Martial d’Auvergne (xive-xve siècles)
Authors: Alamenciak Marie-Antoinette
Published: February 2025

La thèse entend revenir sur les acquis de la théorie littéraire concernant la notion de personnage à partir d’un corpus composé de trois recueils de la fin du Moyen Âge : les Quinze joies de mariage, les Cent Nouvelles nouvelles, les Arrêts d’Amour. Quand la littérature médiévale est prise en compte par la théorie, ses personnages sont assimilés au type. Il s’agit donc de s’interroger sur la généalogie théorique et critique qui a engendré la valorisation d’un certain personnage – celui du roman du xixe siècle – pour discréditer les personnages médiévaux, et particulièrement ceux de notre corpus. Après avoir dépouillé la notion de type de ses préjugés négatifs modernes, nous étudions les conséquences d’une approche majoritairement générique du corpus. Nous montrons que la fiction – mondes possibles fictionnels, transfictionnalité – permet d’appréhender de manière objective et riche nos personnages. La deuxième partie déconstruit les idées préconçues qui concernent l’écriture du type : non décrit, il serait anonyme et inconsistant. À partir d’une analyse des substantifs et des adjectifs, nous revenons sur ces supposées carences à travers une approche gender, qui nous engage également à interroger le statut social du vêtement. La troisième partie pose la question de la réception à partir de trois terrains d’enquête – la rhétorique, les éditions imprimées, le recyclage narratif – qui débouchent sur une interrogation concernant l’acte cognitif de lecture aux enjeux à la fois esthétiques, émotionnels et sociaux. À travers ce travail, nous espérons fournir des pistes pour une étude plus poussée des personnages mis en scène au sein de textes brefs, quels que soient le genre littéraire et la période envisagés.
Romain Doucet, Sous les ailes de l’archange. Saint Michel à l’épreuve de l’histoire (France, xv-xviie siècle)
Authors: Doucet Romain
Published: February 2025

Cette thèse étudie la destinée de saint Michel dans la France du début de l’époque moderne. Dans le contexte d’une spiritualité multipliant les pratiques dévotionnelles, l’archange, par la place qu’il occupe dans le culte, la croyance et la société, est en effet une figure essentielle de la sainteté à la fin du Moyen Âge. Par ailleurs, il bénéficie du choix des Capétiens de faire de lui, au xve siècle, le véritable défenseur et l’ange tutélaire du roi et de la monarchie française. De ce fait, il n’est pas épargné par la remise en question du culte des saints par les idées réformatrices puis, lors des troubles de religion, par les actes iconoclastes visant les symboles catholiques et royaux. Mais son ancrage ancien et l’idéal victorieux qu’il porte le placent en première ligne dans la reconquête catholique au plus fort des guerres de Religion. Toutefois, cette utilisation politique n’est pas sans effet sur le saint archange qui perd de son éclat au xviie siècle. Prenant appui sur un vaste corpus de sources textuelles et iconographiques, envisagées dans leur grande variété de forme et de thématique, cette recherche entend faire de l’archange un acteur de l’histoire du royaume de France en cette période de bouleversements et ainsi révéler la figure emblématique du providentialisme royal et français qu’est saint Michel.
Is there a balm in religion for the Triple Threat wounds of Mother Africa? -Exploring How the Circle is utilizing religious resources in promoting SRHR
Authors: Adera Godfrey Owino
Published: February 2025

The ‘Triple Threat problem’ of -teenage pregnancy, new HIV infection, and gender and sexual based violence among adolescents and young people still persist today as a septic wound for mother Africa. Intervention strategies such as provision of good quality services and timely as well as balanced information regarding sexual and reproductive health and rights have met a myriad of challenges. This is because such interventions come in the backdrop of religious and cultural rhetoric of taboo and stigma that masquerade as morality imperatives. While the opposition against Adolescents’ and young people’s Sexual and Reproductive Health and Rights (referred to henceforth as AYSRHR) issues is very diverse, in many societies, religious rhetoric and beliefs are a frequently recurring barrier to acceptability and accessibility of essential AYSRH information and services. In Kenya, recent studies have suggested intentional involvement of religious leaders in discourses around AYSRHR in general as an intervention strategy. However, the role of religion in destigmatization and demythologization of anti-SRHR discourses have remained undertheorized. By utilizing Talitha cum hermeneutics of the Circle as a rhetorical criticism framework, this paper shall explore how Circle- Kenya is framing, producing, and disseminating religious public discourses and pedagogies that support the provision of AYSRHR essential services and information. It is hoped that this kind of study will contribute significantly to the body of knowledge on the role of religion in addressing the triple threat challenge of teenage pregnancy, new HIV infection, and gender and sexual-based violence among adolescents, as well as suggesting valuable localized pathways in attainment of Kenya’s Vision 2030 and the global Sustainable Development Goals 3- Ensure healthy lives and promote well-being for all at all ages.
The impact of Climate Change on Women and Mental Health
Authors: Nkoulete Penuel Kpadenou
Published: February 2025

Climate change brings exposure to heat, air, pollution, poorer quality food, and infectious diseases that have a significant direct impact on gender and mental health. It acts as a health risk amplifier and reinforces inequalities. These environmental impacts are multifaceted in their consequences and raise risks of depression, suicide, violent victimization, post-traumatic stress disorder, and various other psychiatric symptoms. Women also suffer increased climate psychological risks. Therefore, this study seeks to examine how climate change has impacted the mental health of women and it explores the role of religion and its effect on women’s mental health. The study employed the scoping review methodology where relevant literature across applicable disciplines comprehensively reviewed the multiple pathways through which climate change interacts with gender and mental health. These climate change-fuel disasters are causing harmful impacts on women’s mental health such as post-traumatic stress disorders (PTSD), depression, anxiety, increased substance use or misuse, and suicidal thoughts. Furthermore, the effects of climate change extend to an individual’s overall well-being. Considering the multiple and interconnected pathways of climate impacts and their influence on mental health determinants, we must inform evidence-based interventions. Thus, appropriate action like social support, family therapy, and bibliotherapy in depression and anxiety. Also, population-level interventions, changes in mental health policies, and psychosocial treatments that center on climate justice can reduce the current and future mental health burden, while simultaneously improving the conditions that nurture well-being and equality. Hence, climate change impacts on mental health and the role religion plays must be given greater attention in policy, practice, and research.
The Potential of the Negative: A gender critique of men and HIV and AIDS in Johanne Masowe weChishanu among the Shona of Zimbabwe
Authors: Sipeyiye (PhD) Macloud , Chirongoma (PhD) Sophia
Published: February 2025

This article makes a gender critical analysis of the Johanne Masowe weChishanu (JMwC) beliefs and practices in the context of HIV and AIDS in Zimbabwe. In current times in sub– Saharan Africa, poverty is both a direct and an indirect effect of HIV that feeds on skewed gender relations. Efforts at forestalling the HIV and AIDS pandemic have often concentrated on empowering women through giving them a voice in negotiating for safer sex with their male counterparts. The approach has not lived up to expectation because it focuses on the powerless partner in sexual interactions to lead prevention initiatives against the transmission of HIV and AIDS. Men have resisted such women led initiatives feeling that their authority is threatened. There is a paradigm shift where focus is now on men in African indigenous religions or men in general. However, focus on the potential of men in African Independent Churches (AICs) in dealing with HIV and AIDS has been limited. Focus has been on men in traditional religions or men in general. Can men in AICs transform or revalue their cultural, moral, and spiritual obligation of dealing with threats to health and well-being in the current crisis of HIV & AIDS? This is a critical question since managing HIV and AIDS through transforming gender relations goes a long way in fighting poverty. This article argues that beliefs held about real manhood among JMwC are vital sites for the much-needed revaluing of religious traditions in combating HIV and AIDS that has critical effect on managing poverty levels in households.
An approach to comprehensive assessment of the post-COVID quality of life in the children
Authors: Makhmutov R. F. , Dubovaya A. V. , Likhobabina O. A.
Published: February 2025

The scientific literature around the world reports on the increasing problem of post-COVID syndrome in children affecting their quality of life. Post-COVID syndrome is a clinical condition that occurs several weeks after an episode of acute COVID-19 infection, which resulted in clinical recovery accompanied by nonspecific neurological symptoms, skin vasculitis, mental disorders and impaired functions of different organs. Significant difficulties in diagnosis and medical and preventive measures are caused by the lack of accurate definitions and diagnostic criteria, which currently allows us to determine post-COVID syndrome as a “diagnosis of exclusion”. The purpose of our present study was to develop an approach to comprehensive assessment of the life quality in children with post-COVID syndrome.
COVID-19 infection was diagnosed on the basis of anamnestic data, medical repotrts, results of clinical, laboratory and instrumental studies, consulting with narrow specialists, in accordance with the “ICD 10th revision”. An objective assessment of QOL was based on a set of indices of general clinical blood and urine tests, biochemical parameters, evaluation of immunological status, cardiovascular system, autonomic nervous system, psychoemotional status, and the state of cognitive functions. We studied the initial vegetative tone, the level of memorization, the stability of attention and the performance dynamics, work efficiency, degree of working ability and mental stability, self-assessment scale for the anxiety levels. To diagnose the psychophysiological state of the persons, determine their requests and needs, expectations and attitudes, volitional abilities and stress coping, to assess relationships with people, analyze sources of stress, anxiety, and internal conflicts, the Lusher method (color test) was used.
The study has shown development of vegetative, psychoemotional and cognitive disorders after COVID-19 infection, which cause significant discomfort in daily life in children and adolescents which persist for a considerable time, which, in turn, significantly reduces the quality of life in children and adolescents with post-COVID syndrome.
The developed method of comprehensive quality of life assessment in the children with post-COVID syndrome is aimed at determining clear objective criteria for assessing the quality of life, thus allowing further evaluation of life quality in this patient population, but also specify medical control over all children and adolescents, and those who suffered from COVID-19, and require further observation by neurologists, psychoneurologists, neuropsychologists, speech therapist, psychologist and psychiatrician.
Effect of Helicobacter pylori infection on bone mineral density in patients with rheumatoid arthritis
Authors: Aleksandrov A. V. , Shilova L. N. , Aleksandrov V. A. , Krasilnikov A. N. , Emelyanov N. I. , Alekhina I. Yu. , Aleksandrova N. V. , Zborovskaya I. A.
Published: February 2025

The ability of Helicobacter pylori (H. pylori) to develop and maintain chronic inflammation, induce general immune response, to affect blood lipid metabolism and insulin resistance, and negative impact on bone mineral density (BMD) requires a closer look at the association between H. pylori infection and secondary osteoporosis (OP) in patients with rheumatoid arthritis (RA). The effects of H. pylori infection on BMD at the axial skeletal sites were assessed in 85 women with RA (35 patients were diagnosed with OP). Blood serum samples from RA patients were tested for the presence of IgG class antibodies to H. pylori (anti-Hp-IgG) and total antibodies to H. pylori CagA antigen (anti-CagA). When anti-Hp-IgG was determined, a positive result was recorded in 70.6% of cases. Anti-CagA was also detected in 34 patients with chronic H. pylori infection. In the total group of patients infected with H. pylori, there was a decrease in spinal BMD at the level of L1-4 (BMDL1-L4, p = 0.008), but not in total hip BMD (BMDTotal) (p = 0.06). The analysis of variance demonstrated a significant decrease in both BMDL1-L4 (p = 0.01) and individual BMD parameters of the proximal femur (BMD of the femoral neck, BMDNeck, p = 0.048 and BMD in the Ward’s zone, BMDWards, p = 0.02) detectable only among patients positive for anti-CagA. Among RA patients with a confirmed diagnosis of OP, the incidence of H. pylori infection was significantly higher than in the group of RA patients without osteoporosis (÷2, p = 0.021), and the major proportion of patients with OP were also positive for anti-CagA (73.3%). In patients with significantly high titers of anti-CagA (n = 16), the decrease in BMD was most pronounced and differed from the BMD values for axial skeletal support sites in the group of patients with low titers of these antibodies (BMDL1-L4, p = 0.003; BMDNeck, p = 0.011; BMDTotal, p = 0.049). Infection with H. pylori strains expressing CagA may be considered not only as a risk factor of osteopenia and osteoporosis, but also as a possible risk factor for low-energy fractures in RA patients. Moreover, it seems that the high anti-CagA titers may predict a risk of developing OP in H. pylori-infected patients with RA, rather than simple detection of helicobacteriosis or infection with a CagA-expressing strain. In summary, the presence of chronic H. pylori infection is accompanied by a decrease in BMD across axial skeletal support sites, and H. pylori infection should not be ignored in the treatment of osteoporotic complications in patients with RA.
Allergy mapping in immunocompromised patients and patients with helminthiasis in the Republic of Ingushetia
Authors: Pugoeva Kh. B. , Maksimova A. V. , Tataurschikova N. S.
Published: February 2025

Allergy mapping is a modern technique for diagnosing allergic diseases in immunocompromised patients. Frequent recurrent respiratory viral infections lead to altered classic course in allergic diseases. The aim of the study was to assess the prevalence and spectrum of sensitization in immunocompromised patients and patients with helminthiasis in the Republic of Ingushetia. Allergological examination of patients included prick tests, a blood test for specific IgE using ImmunoCAP. This study was conducted to study the prevalence and type of sensitization in 30 immunocompromised patients aged 18 to 55 years suffering from AR, AtD, PA. Of them, 23 subjects (77%) suffered from allergies and chronic viral infections (CMV, EBV, type 6 HHV, type 1 HSV), with frequent acute respiratory infections, 7 patients (23%) suffered from allergies and parasitic invasions. Food allergies were detected in 7 cases. In these patients, sensitization to Bos d 4 was detected in 57%; to Gal d 1.5, in 43%; to Tri a 14, in 43%; to Mus a, 29%; to Ory 5, in 29% of cases. Most often, patients are susceptible to sensitization to the allergen components of weeds Amb a 1 (30% of cases). Sensitization to beech trees was 17%. Sensitization to the cereal allergens was detected 2 times less often than to trees: 8% of patients were sensitized to the major component of the allergen of Timothy pollen Phl p 1. Most often, patients with helminthiasis were sensitized to food allergens. Of these, Bos d4 sensitization was detected in patient 1 (45%) and patient 4 (50%). Der f 1 demonstrates high sensitization in the patient in patients 6 and 3 up to 30%. Patient 2 exhibited a wide range of allergen sensitization, with significant values for Tri a 14 (35%), Mus a (15%), Bet v 1 (10%) and Phl p 1 (25%). High sensitization to Gal d 1 is observed in patient 3 (30%) and patient 5 (35%). These patterns of sensitization are associated with impaired immunity of intestinal mucosa in the patients with helminthic invasion, which, in turn, is a risk factor for development of sensitization to food allergens.
A new marker of eosinophilic inflammation in patients with chronic obstructive pulmonary disease
Authors: Kuznetsov V. D. , Kozlova Ya. I. , Sobolev A. V. , Frolova E. V. , Uchevatkina A. E. , Filippova L. V. , Vasilieva N. V.
Published: February 2025

Chronic obstructive pulmonary disease (COPD) is one of the most common bronchopulmonary diseases. Eosinophilic inflammation of the respiratory tract in COPD requires further search for new markers for diagnosis and determination of further therapy strategy. Aim: determination of periostin level and assessment of its significance as a marker of eosinophilic inflammation in patients with COPD.
The study included 45 patients with COPD (males at median age of 65). Medical history, results of clinical and instrumental studies were evaluated. The levels of total IgE and periostin were determined in blood serum by enzyme immunoassay. The obtained data were processed using the software systems STATISTICA 13 and SPSS Statistical 27.
On the basis of peripheral blood eosinophils, the patients were divided into a group with eosinophilic inflammatory endotype (9 patients, males aged 67), and a non-eosinophilic endotype (36 patients, men aged 65 years). The patients with eosinophilic endotype of COPD had a later onset of the disease [64 (61-65) years vs 56 (49-60); p = 0.011], and a significantly higher proportion of patients with a history of atopy (80% vs 0%; p 0.001). In patients with non-eosinophilic COPD endotype, a significantly lower FEV1/FVC index was found [46 (44-51) % vs 54 (54-73) %; p = 0.019]. The serum periostin level in patients with the eosinophilic endotype of COPD was 21 (20-22) ng/mL thus being significantly higher than in the group with the non-eosinophilic endotype [14.5 (12-18) ng/mL; p = 0.008]. Using ROC analysis, it was found that a periostin value of more than 19.5 ng/mL is the optimal cut-off point for detecting the eosinophilic endotype in patients with COPD thus serving an additional potential predictor of a positive response to inhaled glucocorticosteroid therapy (ICS) [AUC was 0.940 ± 0.060 with 95% CI: 0.822-1,000, sensitivity of 80%, specificity of 80% (p = 0.007)].
Periostin is a promising marker of eosinophilic inflammation in patients with COPD. Its elevated level may be considered an additional criterion for the administration of ICS therapy.
Point mutations in the mitochondrial genome of skeletal muscle in patients with osteoarthritis
Authors: Goncharov A. G. , Jigkaev A. Kh. , Lobanova V. V. , Kozenkov I. I. , Khaibulin E. V. , Popadin K. Yu. , Gunbin K. V.
Published: February 2025

Osteoarthritis (OA) is one of the most common diseases of the musculoskeletal system, accomplished by a high level of disability. The leading pathogenetic factors of these age-associated diseases include the interrelated processes of “inflammatory aging” and mitochondrial dysfunction, which lead to the development of chronic inflammation and degradation of different joint tissues. The present article contains results about point mutations in mitochondrial genome of peri-articular muscular tissues in the patients with primary OA (experimental group) and post-traumatic osteoarthritis (control group). The study involved 67 volunteers over 53 years old with basic diagnosis of post-traumatic or primary gonarthrosis / coxarthrosis stage 3. Clinical diagnosis was made on the basis of medical history, complaints, clinical and X-ray examination data. The material for the study included the samples of muscles (80 to 100 mm3) obtained at knee or hip replacement surgery. Several techniques have been adapted for isolation, enrichment and purification of nucleic acids, thus allowing to obtain up to 500 ng of mitochondrial DNA (mtDNA) from the biopsies. The prepared mtDNA libraries were sequenced at NGS platform. Bioinformatic analysis was carried out using the following programs: MitoHPC (to detect rare single-nucleotide mutations of mtDNA), MitoSAlt (to detect rare deletions at the mtDNA level) and Splice-Break2 (to detect rare deletions at the level of mtDNA RNA transcripts). Common point mutations A189G (adenine to guanine at position 189) and T408A (thymine to adenine at position 408) were detected. In the control group, the A189G mutation was revealed in 7 patients and T408A mutation was found in 8 volunteers (both mutations were detected in 6 out of 9 persons). In experimental group, the A189G mutation was found in 43 of 58 patients, T408A – in 35 volunteers. In this group of volunteers, both mutations were registered in 19 subjects. The level of mutation frequency, expressed as allele frequency (VAF) in the experimental group significantly exceeded that of the control group. Moreover, in experimental group, unlike control group, a significant correlative relationship was established between the presence of an increased level of mutations in the mitochondrial genome, and a number of clinical and laboratory parameters in volunteers. The described mutations in the mitochondrial genome of periarticular muscle tissue seem to be associated both with aging process and with the direct development of age-associated pathology, i.e., osteoarthritis. The increased levels of mutations in positions 189 and 408 of the regulatory region of mitochondrial genes detected in our patients are apparently associated with both increased level of mutations typical of pathological aging and, possibly, with genotoxic effects of high-dose therapy with non-steroidal anti-inflammatory drugs on mitochondrial genome.
Features of the autoantibody profile in women with systemic lupus erythematosus and endometriosis
Authors: Aleksandrova N. V. , Zborovskaya I. A. , Aleksandrov A. V. , Korenskaya E. G. , Emelyanov N. I.
Published: February 2025

Endometriosis is a chronic inflammatory disease of women, occurring primarily during reproductive age, with high incidence of various autoimmune diseases such as systemic lupus erythematosus (SLE). SLE is characterized by a variety of clinical manifestations and hyperproduction of various antinuclear antibodies. Their role in pathogenesis of endometriosis is actively discussed. We have collected clinical and laboratory data of 53 women (mean age 39.5±9.3 years) with chronic SLE who had gynecological reports on negative or positive assessment of endometriosis, in order to evaluate the patterns of autoantibody profile in women with SLE, dependent on the presence of endometriosis. We determined the following antibodies: antinuclear factor (ANF) by indirect immunofluorescence on HEp-2 cells; antibodies to double-stranded DNA (anti-dsDNA), antibodies to Smith antigen (anti-Sm-IgG), to cardiolipin (IgG/IgM), and to β2-glycoprotein-I (anti-β2GP-I) using appropriate immunoassay kits. The levels of anti-DNA and anti-Sm-IgG were higher in subjects with endometriosis, but the differences did not reach statistical significance (p = 0.068 and p = 0.079, respectively). Similarly, no intergroup differences were observed for antibodies to cardiolipin of different classes and anti-β2GP-I (p 0.1). A higher proportion of patients with positive ANF titers ( 1:160) was found in the group of SLE patients with endometriosis (p = 0.034), with antibody titer 1:320 found in 64.7% of these patients. All ANF-positive samples were diluted to a final titer of 1:1280 and characterized by the type of nuclear staining. The most frequent pattern was homogeneous (45.3%) and granular (41.5%), which combined fine-granular, coarse-granular, and dense fine-granular types of luminescence. Granular type of luminescence was more frequently detected in patients with endometriosis (11/17 vs. 11/36; χ2Yates, p = 0.04), reflecting the reaction of autoantibodies with nucleoprotein complexes. When several types of ANF luminescence were detected in the same sample, one of them (usually at low titers) interfered with others, and changed when the dilution titer was increased to 1:640 – 1:1280 (9 individuals with different transition variants). It seems that the characterization of ANF luminescence type may provide more valuable information in future management of SLE patients with comorbid pathology. Further studies are needed to evaluate the role of ANF in pathophysiologic mechanisms of endometriosis in SLE.
Ambivalent in vitro effect of immunoregulatory peptides on antigen-presenting subsets of neutrophil granulocytes in purulent inflammatory diseases
Authors: Nesterova I. V. , Chudilova G. A. , Teterin Yu. V.
Published: February 2025

Neutrophilic granulocytes (NG) are functioning as regulators of the immune response. Expression of NG molecules HLA-DR and presentation of antigen to T cells is one of their regulatory mechanisms. The NG dysfunction plays a great role in pathogenesis of acute hematogenous osteomyelitis (AHO) in children. An activated, antigen-presenting NG subset (APC) СD66b+CD16+CD33+HLA-DR+ was also found in these patients. Therefore, studies of surface NG membrane receptor expression, including HLA- DR, their regulation by peptides, and influence of the latter factors on correction on NG effector functions are of sufficient interest. Our objective was to evaluate the possibility of in vitro modulating the phenotype of CD66b+CD16+CD33+HLA- DR-, CD66b+CD16+CD33+HLA-DR+ subsets of neutrophilic granulocytes under the influence of hexapeptide (HP) and glucosaminylmuramyl dipeptide (GMDP) in blood cells of children with acute hematogenous osteomyelitis using in vitro experimental tests.
Peripheral blood (PB) of 28 children with AHO aged 8-15 years was studied (the study group). 13 healthy children aged 8-15 years comprised the comparison group. To evaluate the effect of peptides, PB of children with AHO was cultured with HP (10-6 g/L, 60 min, 37 °С): study group 1, and with GMDP (10-6 g/L, 60 min, 37 °С) – study group 2. The number of NG CD66b+CD16+CD33+HLA-DR+, CD66b+CD16+CD33+HLA- DR- subsets, receptor expression density (MFI) (FC 500 “Beckman Coulter”, USA), phagocytic activity of NG, before and after cultivation were tested with these peptides.
In children with AHO, a subset of NG CD66b+CD16+CD33+HLA-DR+ is registered in 30.2 (16.4-34.9) %; with MFI, HLA-DR it comprised 3.5 (3.3-4.2) %. Under the influence of HP, a decrease of NG-APC and MFI HLA-DR numbers to 1.7 (1.6-2.2) (p1.2 0.05) was revealed, due to binding of HP to HLA-DR (p 0.05). Under the influence of GMDP, there is a significant increase in MFI CD66b and MFI CD33 receptors (p1.2 0.05) in both subsets; there is an increase in MFI HLA-DR (p 0.05) in the NG-APC subset. The modulating effects of HP and GMDP on the phenotype of NG CD66b+CD16+CD33+HLA-DR+ and CD66b+CD16+CD33+HLA- DR- subsets may contribute to restoration of the phagocytic function of NG.
We have detected the “long-lived” activated NG subset CD66b+CD16+CD33+HLA-DR+ with the properties of APC, that can present antigen to T lymphocytes in PB of children with AHO. However, the important question exists, whether such a transformation will promote or slow down the progression of the purulent-inflammatory process? In this study, we have demonstrated in vitro the ability of two immunotropic peptides (HP, GMDP) to modulate the phenotype of NG-APC subset, thus potentially promoting recovery of the NG effector functions.
Expression of "non-classical" MHC molecules in bronchial asthma and atopic dermatitis
Authors: Boeva O. S. , Borisevich V. I. , Abbasova V. S. , Kozlov V. A. , Demina D. V. , Pashkina E. A.
Published: February 2025

Currently, bronchial asthma (BA) and atopic dermatitis (AD) are among the most common allergic diseases. According to available data, the worldwide incidence of bronchial asthma is about 260 million people. Prevalence of BA in adult population is 6.9%, and about 10% among children and adolescents. AD occurs in approximately 10-30% of children and 2-10% of adults in developed countries. Every year, a steady increase in AD and BA incidence is noted, but the pathogenesis of these diseases remains poorly known. Recently, the research is being devoted to the studies of interactions between the immune cells and molecules of the major histocompatibility complex (MHC, human leukocyte antigens, HLA), especially, to “non-classical” HLA. HLA-E are among the less studied antigens. Its gene is located in the short arm of chromosome 6 between the HLA-A and HLA-C genes. HLA-E transcription is found in almost all cell types. HLA-E expression is predominantly present on the surface of lymphoid cells (T and B lymphocytes, monocytes and macrophages), to greater extent, and, to lesser degree, on the surface of endothelial cells. Currently, the role of HLA-E in allergic disorders has not been described in the literature. Therefore, it seems relevant to study the role of HLA-E in allergic diseases, in particular, in bronchial asthma and AD. The biological material for this study were PBMCs taken from the patients with bronchial asthma (n = 19), AD (n = 7) and healthy donors (n = 16). We have found that the proportion of T helper cells carrying surface HLA-E was decreased in allergic disorders when compared to healthy donors. At the same time, the level of HLA-E expression in patients with atopic dermatitis was significantly lower than in patients with bronchial asthma. The level of HLA-E expression on CD14+ and CD8+T cells was significantly lower in patients of the AD group compared to the donor group, whereas no statistically significant differences were observed between the AD group and the donor group. Thus, the level of HLA-E expression on immunocompetent cells differed between the group of donors and the patients with Th2 diseases. A significantly decreased expression of this molecule on immunocompetent cells of the patients with bronchial asthma may suggest a possible role of this molecule in pathogenesis of this disease.
C. difficile-associated enterocolitis in a child: a clinical case
Authors: Abyanova P. I. , Matiyeuskaya N. V. , Klyuchnik E. V.
Published: February 2025

Antibiotic-associated diarrhea (AAD) is one of the most significant complications of antibacterial treatment. The objective of the study was to provide a clinical case of C. difficile-associated enterocolitis in a child. Case description. A clinical case of moderate enterocolitis caused by C. difficile is presented in a 4-year-old child who fell ill while taking 3 courses of antibiotics (cefotaxime, claptrithromycin, amikacin), which manifested as abdominal pain, false attempts to defecate, frequent scanty mucous stool with blood admixture, fever. At the same time, negative results were obtained from bacteriological stool culture for enteropathogenic microflora and stool testing for intestinal viruses using ELISA and PCR; a questionable result was obtained at stool testing for the type A and B C. difficile toxins. A general blood test showed neutrophilic leukocytosis and a left shift in the differential leukocyte counts, inflammatory changes in the coprogram, thickening of the colon wall up to 3-4.5 mm according to ultrasound data, enlarged mesenteric lymph nodes were found. Upon anti-infectious therapy with vancomycin and metronidazole, rapid positive dynamics of the child’s condition and recovery were noted. Conclusions. The diagnosis of C. difficile-associated infection should be established on the basis of a comprehensive analysis of available clinical, epidemiological, anamnestic, laboratory and instrumental data. Negative or questionable results of the test for C. difficile toxins A and B in feces does not exclude an infection associated with C. difficile.
Personalized diagnostics and therapy of children with allergopathology in the Kaliningrad region
Authors: Marhaichuk A. Z. , Gorbunova A. Yu. , Goncharov A. G. , Tuzankina I. A.
Published: February 2025

Allergopathology is a multifactorial group of diseases that includes clinical manifestations from the skin, gastrointestinal and respiratory systems. This group of diseases is based on the mechanisms of hypersensitivity types I, V and VI. These disorders can manifest with similar clinical features. In addition, they must be differentiated from infectious syndrome of primary immunodeficiencies, with onset of an autoimmune disorder, rhinopathy associated with endocrine diseases, enzymopathies. The cohort of children with allergic rhinitis is heterogeneous, due to potentially variable course of hypersensitivity and combination of various pathogenetic pathways. The aim of our study was to develop a personalized approach to diagnosis and therapy in children with manifestations of rhinitis and prolonged cough syndrome. The study included a group of 53 children aged 2 to 18 years who consulted an allergist-immunologist with leading complaints for nasal congestion and prolonged cough. We also excluded infectious factors of hypersensitivity. According to the results of examination and anamnesis, rhinitis was combined with postnasal drip in all patients (n = 53, 100%); episodes of broncho-obstruction and laryngospasm were observed in 20 patients (24%), bronchial asthma attacks – in 7 children (13.2%). Polyresistant bacterial microflora was detected in 20 patients. Allergosensitivity was revealed in 31 children. Rhinopathy associated with alactasia was found in 8 children, with hypolactasia against the background of regular consumption of dairy products – in 5 children. Sanation of chronic flora and dietary therapy was followed by remission of rhinopathy. In 7 patients, the iron deficiency conditions led to sensitivity to bacterial flora. In the structure of sensitization, allergens of house dust mites predominated (61.3%), along with food sensitization (48.4%), epidermal allergens of animals (45.2%), plant allergens (54.67%). In the cases where we identified diagnostically significant allergens, allergen-specific immunotherapy with sublingual (SLIT) drugs was recommended to children. During the year of observation, 12 subjects underwent treatment with house dust allergens; 5, with birch allergens; 3, with a mixture of meadow grass allergens, and one child with wormwood. Some patients still needed pharmacotherapy. After the first course of SLIT, all patients experienced a significant relief of the syndrome. Thus, personalized diagnostics should include a thorough examination: allergy diagnostics, exclusion of gastrointestinal pathology and enzymopathies, detection and sanitation of chronic foci of infections, including parasitic invasions. Such an algorithm may significantly alleviate the severity and duration of clinical manifestations of rhinopathy.