bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on Financing Strategy of Green Energy-Efficient Supply Chain Based on Blockchain Technology
Authors: Wang Di , Zhao Daozhi , Chen Fang
Published: March 2023

With the development of ecological economics, energy-saving green energy chain management has been a wide concern of academia and industries. However, the relatively high cost of green investment makes manufacturers face the problem of financial constraints. On this basis, because the green level information of products is proprietary to manufacturers, manufacturers will lie about the green level of products in order to improve their profits out of the principle of profit maximization. As a result, banks cannot obtain the true green level of products, reducing the benefits of the green energy-efficient supply chain system and making the market of green products volatile. In view of this, blockchain technology is introduced in this paper to improve customer’s product green level sensitivity and obtain lower green credit interest rates from banks. In this paper, a green supply chain financing model based on blockchain technology was constructed under the condition of green information misreporting, and it is compared with the benchmark without blockchain technology. Research shows that the adoption of blockchain can achieve Pareto improvement of green supply chain members. In addition, manufacturers have an incentive to adopt blockchain if the cost of blockchain investment falls below a certain threshold, and consumer green sensitivity increases below that threshold. We compared the profits of green manufacturers with those of retailers and the total emissions of manufacturers. The results show that: (1) When the financing intensity exceeds a certain value, there is an optimal coverage of green financing to ensure that the profit target of manufacturers, the profit target of retailers and the emission reduction target are achieved simultaneously. (2) The adoption of blockchain can achieve Pareto improvement of green energy supply chain members. The actual data of green transformation of Jinyuan New Technology Company were cited. Through calculation, it was found that green transformation can reduce the emissions of enterprises. When the financing intensity is in a certain range, the profits of manufacturers and retailers can be maximized, and the emission reduction degree is the highest. Thus, the practicability and reliability of this model were proved. (3) Manufacturers have an incentive to adopt blockchain if the cost of blockchain investment falls below a certain threshold, and consumer green sensitivity increases below that threshold. The research results of this paper provide solutions for enterprises with limited funds for green transformation and provide a theoretical basis for the government to formulate emission reduction incentive mechanism.
Application of Nanoparticles in Bioreactors to Enhance Mass Transfer during Syngas Fermentation
Authors: Sajeev Evelyn , Shekher Sheshank , Ogbaga Chukwuma C. , Desongu Kwaghtaver S. , Gunes Burcu , Okolie Jude A.
Published: March 2023

Gas–liquid mass transfer is a major issue during various bioprocesses, particularly in processes such as syngas fermentation (SNF). Since SNF involves the movement of gases into the fermentation broth, there is always a rate-limiting step that reduces process efficiency. Improving this process could lead to increased efficiency, higher production of ethanol, and reduced energy consumption. One way to improve fluid transfer between gas and liquid is by incorporating nanoparticles (NPs) into the liquid phase. This entry describes recent advances in using NPs to improve gas–liquid mass transfer during SNF. The entry also describes the basics of SNF and the impact of NPs on the process and suggests areas for future research. For example, carbon nanotubes have been found to elevate the available surface area needed for gas–liquid transfer, thus improving the process efficiency. Another area is the use of NPs as carriers for enzymes involved in syngas fermentation.
Mediating Effect of Bio-Organic Fertilizer on the Physiological Characteristics of “Qi-Nan” Agarwood from Aquilaria sinensis (Lour.)
Authors: Huang Jingyue , Zhao Zhang , Bai Tiandao , Xiong Junfei , Li Yingjian , Wei Penglian , Fu Yunlin
Published: March 2023

This study examines the impact of bio-organic fertilizers on the physiological characteristics and leaf nutrient content of “Qi-Nan” agarwood seedlings. The aim is to provide a theoretical foundation for the efficient cultivation of “Qi-Nan”. The experiment was conducted under field conditions using a Randomized complete block design. The control group received no fertilization, while the three fertilizer-treated groups received vermicompost, sheep manure, or microbial organic fertilizer. The results indicate that the application of bio-organic fertilizer significantly promoted the growth of plant height and diameter, increased the contents of soluble proteins, soluble sugar, chlorophyll, and leaf nutrients; and decreased the contents of free proline, peroxidase, malondialdehyde, and other stress indices. Redundancy analysis showed that the main factors affecting the seedlings height and diameter were free proline, chlorophyll, total nitrogen, and total potassium. Principal component analysis and membership function analysis revealed significant variations in growth, physiological characteristics, and nutrient content among the three fertilizer groups and the control group, with vermicompost > microbial organic fertilizer > sheep manure > no fertilization. In conclusion, biological organic fertilizer significantly promotes the growth of “Qi-Nan” seedlings, and vermicompost is the most effective fertilizer in this experiment.
Thinning Levels of Laurel Natural Regeneration to Establish Traditional Agroforestry Systems, Ecuadorian Amazon Upper Basin
Authors: Cañadas-López Álvaro , Gamboa-Trujillo Paul , Buitrón-Garrido Santiago , Medina-Torres Byron , Vargas-Hernández J. Jesús , Wehenkel Christian
Published: March 2023

(1) Background: The Cordia alliodora (Ruiz & Pav.) Oken (laurel) natural regeneration management is a widespread practice among smallholders in the Amazon upper basin for the establishment of traditional agroforestry systems. This tree management approach is opposite to the development project proposals that contemplate reforestation with nursery seedlings in the Amazon region. The present study evaluated the effects of thinning levels on the diameter and basal area increment of laurel in a traditional agroforestry system; (2) Methods: A randomized complete block design with three replications was used, and the target variables were the growth rate of diameter at breast height (DBH) and the basal area of trees. Twelve square field plots, 400 m² each, were established in a plot network covering a 1.0 km × 0.5 km area. Three thinning levels were applied: light, moderate, and intensive thinning; (3) Results: The traditional agroforestry system investigated was characterized by a marginal growth of laurel trees, with an average yield of 125.26 m3 ha−1 (±15.39) and MAI of 13.92 m3 ha−1 at 9 years of age with a tree density of 418 trees ha−1. The average value of the relation between the number of trees and the basal area removed (NG value) was 1.15, with small variation among plots, so all of them were thinned from below. The intensive thinning treatment, leaving 200 trees ha−1, caused the greatest annual increase in individual tree DBH (2.03 cm) and basal area (61.37 cm2) in both absolute and relative terms and improved tree height/diameter ratio; (4) Conclusions: Management of laurel natural regeneration for the establishment of traditional agroforestry systems could be improved by thinning at early ages, leaving 200 well-spaced laurel trees per ha.
Online Machine Vision-Based Modeling during Cantaloupe Microwave Drying Utilizing Extreme Learning Machine and Artificial Neural Network
Authors: Zhu Guanyu , Raghavan G. S. V. , Xu Wanxiu , Pei Yongsheng , Li Zhenfeng
Published: March 2023

Online microwave drying process monitoring has been challenging due to the incompatibility of metal components with microwaves. This paper developed a microwave drying system based on online machine vision, which realized real-time extraction and measurement of images, weight, and temperature. An image-processing algorithm was developed to capture material shrinkage characteristics in real time. Constant-temperature microwave drying experiments were conducted, and the artificial neural network (ANN) and extreme learning machine (ELM) were utilized to model and predict the moisture content of materials during the drying process based on the degree of material shrinkage. The results demonstrated that the system and algorithm operated effectively, and ELM provided superior predictive performance and learning efficiency compared to ANN.
The Effect of Direct and Indirect Barbecue Cooking on Polycyclic Aromatic Hydrocarbon Formation and Beef Quality
Authors: Sumer Gulsah , Oz Fatih
Published: March 2023

Herein, the effect of direct and indirect barbecue cooking processes, including different cooking degrees (medium and well done), on the formation of polycyclic aromatic hydrocarbons (PAHs) and on certain quality parameters (water content, cooking loss, pH, lipid oxidation) of beef meat was examined. While no significant effect (p > 0.05) of the cooking method was detected on the water content, cooking loss, ∑PAH4 [the sum of benzo[a]pyrene (BaP), benzo[a]anthracene (BaA), chrysen (Chry), and benzo[b]fluoranthene (BbF)], and ∑PAH8 [the sum of BaP, BaA, Chry, benzo[k]fluoranthene (BkF), dibenzo[a,h]anthracene (DahA), benzo[g,h,i]perylene (Bghip), and indeno [1,2,3-cd]pyrene (IncdP)] content, it was determined that it had a significant effect on pH (p < 0.05) and lipid oxidation (TBARS, p < 0.01). While the cooking degree did not have a significant effect (p > 0.05) on the TBARS value, it had a significant effect (p < 0.05) on the water content and pH value, and a very significant effect (p < 0.01) on the cooking loss. While BaA and BaP compounds were detected in all barbecued samples, the DahA compound could not be detected in any of the samples. Varying levels of BaA (up to 5.62 ng/g), Chry (up to 0.43 ng/g), BbF (LOD-..-LOQ), BkF (LOD-..-LOQ), BaP (up to 0.49 ng/g), BghiP (up to 0.82 ng/g), and IncdP (up to 4.99 ng/g) compounds were determined in the samples. While the ∑PAH4 contents varied between 0.71 and 6.35 ng/g, the ∑PAH8 contents varied between 1.12 and 11.34 ng/g. While the increase in cooking level did not affect the ∑PAH4 content, it caused a significant increase in the ∑PAH8 content. The highest BaP (0.49 ng/g), ∑PAH4 (6.35 ng/g), and ∑PAH8 (11.34 ng/g) contents were detected in the meat samples that were well cooked on the barbecue by the direct method. The results have proven that PAHs are formed at varying levels in both meat samples cooked on the barbecue by the direct method and the indirect method. On the other hand, it has been determined that even if 100 g of the meat with the highest BaP and ∑PAH4 content is eaten, the exposure amount remains far below the limit values specified for PAHs. However, paying close attention to the barbecue cooking process is still recommended.
Changes in Texture Characteristics and Special Requirements of Sichuan-Style Braised Beef for Industrial Production: Based on the Changes in Protein and Lipid of Beef
Authors: Pu Xiaoli , Ruan Jinggang , Wu Zhicheng , Tang Yong , Liu Ping , Zhang Dong , Li Hongjun
Published: March 2023

This study aimed to investigate the optimal stewing time (0, 30, 60, 90, 120, and 150 min) for industrialized preparation of Sichuan-style braised beef with different demands. With prolonged stewing time, the hardness and chewiness of the braised beef initially increased and then decreased (p < 0.05), whereas springiness and cohesiveness gradually decreased. The moisture content of braised beef and the endogenous fluorescence intensity of braised beef protein significantly decreased (p < 0.05). However, the thiobarbituric acid reaction substances (TBARS) value and protein carbonyl content of braised beef greatly increased (p < 0.05). During the stewing process, the texture properties of Sichuan-style braised beef were affected by the moisture content, oxidation of proteins and lipids, and integrity of the muscle fibers. Considering texture traits, when Sichuan-style pre-braised beef bought by consumers is stewed with other ingredients for about 30 min, its corresponding stewing time is 60 min in industrialized production processes. This process parameter can not only save energy consumption for practical production, but also improve the hardness value of the as-obtained Sichuan-style pre-braised beef, which is conducive to transportation through refraining from cracking of pre-braised beef pieces. When consumers only use simple heating to eat the Sichuan-style pre-braised beef product, stewing times of 120 or 150 min can be considered in industrialized production processes. This work provided a theoretical reference for the industrialized and standardized production of different types of prepared Sichuan-style braised beef.
A Note on k-Bonacci Random Walks
Authors: Attia Najmeddine , Saidi Neji , Souissi Chouhaïd , Ali Rifaqat
Published: March 2023

In this work, the probability of return for random walks on Z, whose increment is given by the k-bonacci sequence, is determined. Additionally, the Hausdorff, packing and box-counting dimensions of the set of these walks that return an infinite number of times to the origin are given. As an application, we study the return for tribonacci random walks to the first term of the tribonacci sequence.
A Yin and Yang Perspective on the Trust Game: Trust and Reciprocity
Authors: Krueger Frank
Published: March 2023

Trust and reciprocity are fundamental for the cohesion and stability of human society, as they are essential components of cooperative exchange [...]
Copper-/Zinc-Doped TiO2 Nanopowders Synthesized by Microwave-Assisted Sol–Gel Method
Authors: Predoană Luminița , Petcu Gabriela , Preda Silviu , Pandele-Cușu Jeanina , Petrescu Simona Viorica , Băran Adriana , Apostol Nicoleta G. , Costescu Ruxandra M. , Surdu Vasile-Adrian , Vasile Bogdan Ştefan , Ianculescu Adelina C.
Published: March 2023

Using the microwave-assisted sol–gel method, Zn- and Cu-doped TiO2 nanoparticles with an anatase crystalline structure were prepared. Titanium (IV) butoxide was used as a TiO2 precursor, with parental alcohol as a solvent and ammonia water as a catalyst. Based on the TG/DTA results, the powders were thermally treated at 500 °C. XRD and XRF revealed the presence of a single-phase anatase and dopants in the thermally treated nanoparticles. The surface of the nanoparticles and the oxidation states of the elements were studied using XPS, which confirmed the presence of Ti, O, Zn, and Cu. The photocatalytic activity of the doped TiO2 nanopowders was tested for the degradation of methyl-orange (MO) dye. The results indicate that Cu doping increases the photoactivity of TiO2 in the visible-light range by narrowing the band-gap energy.
tsRNA Landscape and Potential Function Network in Subcutaneous and Visceral Pig Adipose Tissue
Authors: Wang Linghui , Gu Hao , Liao Tianci , Lei Yuhang , Qiu Yanhao , Chen Qiuyang , Chen Lei , Zhang Shunhua , Wang Jinyong , Hao Xiaoxia , Jiang Dongmei , Zhao Ye , Niu Lili , Li Xuewei , Shen Linyuan , Gan Mailin , Zhu Li
Published: March 2023

Noncoding RNAs (ncRNAs) called tsRNAs (tRNA-derived short RNAs) have the ability to regulate gene expression. The information on tsRNAs in fat tissue is, however, limited. By sequencing, identifying, and analyzing tsRNAs using pigs as animal models, this research reports for the first time the characteristics of tsRNAs in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT). A total of 474 tsRNAs, 20 and 21 of which were particularly expressed in VAT and SAT, respectively, were found in WAT. According to the analysis of the tsRNA/miRNA/mRNA co-expression network, the tsRNAs with differential expression were primarily engaged in the endocrine and immune systems, which fall under the classification of organic systems, as well as the global and overview maps and lipid metropolis, which fall under the category of metabolism. This research also discovered a connection between the activity of the host tRNA engaged in translation and the production of tsRNAs. This research also discovered that tRF-Gly-GCC-037/tRF-Gly-GCC-042/tRF-Gly-CCC-016 and miR-218a/miR281b may be involved in the regulation of fatty acid metabolism in adipose tissue through SCD based on the tsRNA/miRNA/mRNA/fatty acid network. In conclusion, our findings enrich the understanding of ncRNAs in WAT metabolism and health regulation, as well as reveal the differences between SAT and VAT at the level of tsRNAs.
Peeping into Mitochondrial Diversity of Andaman Goats: Unveils Possibility of Maritime Transport with Diversified Geographic Signaling
Authors: De Arun Kumar , Sawhney Sneha , Sunder Jai , Muthiyan Ramachandran , Ponraj Perumal , Sujatha Tamilvanan , Malakar Dhruba , Mondal Samiran , Bera Asit Kumar , Kumar Ashish , Chakurkar Eaknath Bhanudasrao , Bhattacharya Debasis
Published: March 2023

Andaman and Nicobar Islands, a part of South-East Asia, is enriched with the presence of native breeds of livestock (cattle, pig, goat) and poultry. There are two native goat breeds, viz., Andaman local goat and Teressa goat in Andaman and Nicobar Islands. However, to date, the origin and genetic makeup of these two breeds have not been detailed. Therefore, the present study describes the genetic makeup of Andaman goats through analysis of mitochondrial D-loop sequence for sequence polymorphism, phylogeographical signaling and population expansion events. The genetic diversity of the Teressa goat was less compared to the Andaman local goat due to its sole presence on Teressa Island. Out of 38 well-defined haplotypes of Andaman goats, the majority of haplotypes belonged to haplogroup A followed by haplogroup B and haplogroup D. The result of mismatch distribution and neutrality tests indicated no population expansion event of haplogroup A and B. Finally, based on poor geographical signaling, we hypothesize that Andaman goats have been imported to these Islands either through multidirectional diffusion or unidirectional diffusion. We justify our hypothesis of multidirectional diffusion on the basis of observation of the haplotype and nucleotide diversity of Andaman goats. Simultaneously, the probability of unidirectional diffusion of goats in these islands from the Indian subcontinent in different spells of domestication events through maritime routes cannot be ignored.
Nature–Human Relational Models in a Riverine Social–Ecological System: San Marcos River, TX, USA
Authors: Lopez Christina W. , Wade Madeline T. , Julian Jason P.
Published: March 2023

A social–ecological system is a highly connected organization of biophysical and social actors that interact across multiple scales, share resources, and adapt to the actors’ changes. The ways in which humans and nature interact have traditionally been characterized and influenced by competing intrinsic and utilitarian values. However, recently, relational values and relational models have been used to unpack the myriad of values society assigns to nature and create general typologies of nature–human relationships. Here, we investigate the spectrum of environmental values that exist in the San Marcos River (SMR)—a social–ecological system (SES) in which a spring-fed river flows through an urban environment in central Texas (USA) including a university campus that attracts regional and international tourists. Recognizing that scholars have struggled to identify a nuanced understanding of environmental values and how these values shape nature–human relationships in SES, we use the SMR case study to capture the nature–human relational models that exist among social and user groups of the blue space. Analyzing different groups of visitors and stakeholders of the SMR (n = 3145), this study serves as a pilot to apply relational models using a variety of metrics to build a framework for understanding models of nature–human relationships, beyond ecosystem services and dualistic valuations. In our sample, most respondents were classified under the stewardship model (59%). The utilization model (34%) was the second most common, followed by wardship (6%). We found that patterns of place identity emerged to support the development of relational models beyond utilization. Despite the differences among perceptions, values, and some variation in relational models, one commonality was the innate, ubiquitous preference to protect natural habitat, water quality, and the river’s aquifer water source. Our study contributes to the growing literature around relational values and is a pathway to integrate ecosystem services, environmental values, and human–environment interactions into a more holistic approach to environmental valuation.
Prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium among Patients with Urogenital Symptoms in Istanbul
Authors: Kirkoyun Uysal Hayriye , Koksal Muammer Osman , Sarsar Kutay , Ilktac Mehmet , Isik Zeynep , Akgun Karapinar Deniz Bahar , Demirci Mehmet , Ongen Betigul , Buyukoren Ahmet , Kadioglu Ates , Yurtsever Eray , Agacfidan Ali
Published: March 2023

Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium are the three most commonly reported sexually transmitted bacteria. The present study aimed to investigate the presence of C. trachomatis, N. gonorrhoeae, and M. genitalium in urogenital samples collected from 18–68-year-old Turkish patients who were admitted to the hospital with various urogenital symptoms. A total of 360 patients with symptoms of STD were included in the study. Following DNA extraction by QIAamp Mini Kit, the presence of C. trachomatis, N. gonorrhoeae, and M. genitalium were investigated using multiplex real-time PCR. Causative organisms were identified in 68 (18.9%) of 360 patients. C. trachomatis, N. gonorrhoeae, and M. genitalium were detected in 40 (11.1%), 14 (3.9%), and 28 (7.8%) of the patients, respectively. Patients 21–30 years of age represented more than one-third (37.8%) of positive patients. Of all patients, dual infections of C. trachomatis–M. genitalium, N. gonorrhoeae–C. trachomatis, N. gonorrhoeae–M. genitalium, and triple infection of C. trachomatis–N. gonorrhoeae–M. genitalium were determined in 1.6% (6/360), 1.3% (5/360), 0.2% (1/360), and 0.2% (1/360) of the patients, respectively. In CT-, NG-, and MG-positive patients, different STI agents were also found such as HIV, HBV, HPV, HSV2, T. pallidum, and T. vaginalis. In conclusion, among C. trachomatis, N. gonorrhoeae, and M. genitalium, CT was the most frequently detected bacterial cause of STDs in our hospital at Istanbul. Co-infections, which comprise more than one-fifth of the cases, should not be underestimated. Regular screening and following up of STD agents using multiplex real-time PCR-based diagnostic methods enabling the immediate detection of co-infections are essential for the treatment and primary prevention of STDs.
The Individual and Combined Effects of Social Networks and Loneliness on Life Satisfaction among Community-Dwelling Residing Older Adults: A Longitudinal Study
Authors: Foong Hui Foh , Ibrahim Rahimah , Hamid Tengku Aizan , Bagat Mohamad Fazdillah
Published: March 2023

Social networks and loneliness are correlates of life satisfaction in old age. However, the evidence of the combined effects of social isolation and loneliness on life satisfaction is lacking; therefore, this study also aimed to investigate the combined effects of social networks and loneliness on life satisfaction in Malaysian older adults. Data from two waves of the “Neuroprotective Model for Healthy Longevity among Malaysian Older Adults” study were extracted. The first wave of data collection was completed in February 2013, while the second wave was conducted three years after the first wave. The main statistical analysis used was multivariable logistic regression. For individual effect, social networks (B = 0.375, p = 0.007), but not loneliness (B = −0.178, p = 0.368) significantly determined life satisfaction. Increasing social network size causes increasing life satisfaction. For combined effects, those in “the lone farmers” group (B = 0.279, p = 0.044) and “the majority” group (B = −0.413, p = 0.004) were linked to life satisfaction. Social engagement in older people is important for wellbeing in later life. Therefore, community programs and investing in a quality relationship should be encouraged to obtain adequate support and ultimately promote higher life satisfaction.
The Relationship between Working Night Shifts and Depression among Nurses: A Systematic Review and Meta-Analysis
Authors: Okechukwu Chidiebere Emmanuel , Colaprico Corrado , Di Mario Sofia , Oko-oboh Agbonvihele Gregrey , Shaholli David , Manai Maria Vittoria , La Torre Giuseppe
Published: March 2023

Background: For many years, occupational physicians have debated whether there is a link between working the night shift and depression and other co-occurring mental health issues, with an emphasis on work-related, biological, individual, and environmental factors. We performed this systematic review and meta-analysis to estimate the overall association between sleep deprivation and depression among nurses working night shifts. Methods: A systematic search was carried out across the electronic databases PubMed, Scopus, and Web of Science from inception to 30 September 2022, for studies that reported a relationship between estimated night shift work and depression in nurses. The outcomes were measured using the odds ratio (OR) and matching 95% confidence interval (CI). The I2 statistic was used to assess heterogeneity. The Grading of Recommendations Assessment, Development and Evaluation technique was used to evaluate the quality of the evidence, and the Newcastle–Ottawa Scale was utilized to assess the methodological quality of each of the included studies. We determined the overall relationship between working nights and the onset of depression. Results: A total of 20 studies were included in the systematic review. Furthermore, 8 studies were included in the meta-analysis due to their common use of the OR as an effect measure. The 8 studies gave an overall estimate indicating a statistically significant association between night shift work and depression among nurses (OR = 1.49 95% CI: 1.26, 1.76). The prediction interval for the overall estimate was (0.995, 2.231). This implies that the true OR in a future study would most likely fall within this range, with a 95% certainty. Conclusions: The outcome of this systematic review and meta-analysis showed a significant association between night shift work, the circadian and sleep disruption it causes, and the risk of depression in nurses. This demonstrates that nurses who work night shifts are at risk of developing depression.
The Situation and Influencing Factors of Depression and Anxiety in Patients of Hemodialysis during the COVID-19 Pandemic in China
Authors: Jiang Dan , Yu Xi , Zhong Tian , Xiao Ying , Wang Ling
Published: March 2023

Objective: To investigate the incidence of depression and anxiety among maintenance hemodialysis (MHD) patients during the regular prevention and control stage of COVID-19 in China as well as the influencing factors. Methods: A cross-sectional study including 180 patients under the treatment of hemodialysis was conducted in the hemodialysis center of the 8th Affiliated Hospital of Sun Yat-Sen University. The questionnaire regarding the subject’s general information, Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS) was completed by the patients, and the laboratory test results were recorded. Results: The incidences of anxiety and depression were 35.6% and 38.9%, respectively, and the average scores of SAS and SDS were (48.03 ± 5.02) and (48.12 ± 5.42), respectively, in the subjects. The results showed that age, monthly income, vascular access of dialysis, feeling of pain and itching (within a week), worried about being infected by COVID-19, whether having health insurance and the levels of hemoglobin, parathyroid hormone, and phosphorus were the impact factors of both anxiety and depression in the MHD patients (p < 0.05 for all). Conclusion: The proportion of depression and anxiety is relatively high in the MHD patients during the regular prevention and control stage of COVID-19.
New Evidence about the Polychromy of Early Imperial Cycle from the Augusteum of Rusellae (Tuscany)
Authors: Liverani Paolo , Bracci Susanna , Iannaccone Roberta , Lenzi Sara , Magrini Donata
Published: March 2023

This study is concerned with recent analyses of seven marble statues from the imperial cycle of the Augusteum of Rusellae, in the south of Tuscany, Italy. The sculptures represent the deified couple Augustus and Livia, Livilla, Claudius, an anonymous young girl and a headless cuirassed emperor (maybe Domitian). In addition, a fragment of a leg, from another cuirassed statue, was also considered. All of them are preserved in the city of Grosseto, in the Maremma Archaeology and Art Museum. Still preserved traces of polychromy and gilding were investigated both in situ, using non-invasive portable techniques, and in laboratory, taking two micro-samples. The non-invasive approach was based on multi-band imaging techniques (Vis, UVL and VIL) integrated with analyses (XRF, reflectance spectroscopy). A portable optical microscopy was also used for documenting the analysed areas. Two micro-samples from the gilding decoration of the headless cuirassed statue were also analysed using EDS-SEM. Comparing the results from the analytical survey, important information about the use of ochres and Egyptian blue on the cuirassed headless Emperor statue has been highlighted with the presence of gilding in the cuirasses and in the mantle, enriching the knowledge of this important imperial cycle, in addition to contributing to the archaeological point of view.
Do Multiple Sex/Gender Dimensions Play a Role in the Association of Green Space and Self-Rated Health? Model-Based Recursive Partitioning Results from the KORA INGER Study
Authors: Dandolo Lisa , Telkmann Klaus , Hartig Christina , Horstmann Sophie , Pedron Sara , Schwettmann Lars , Selsam Peter , Schneider Alexandra , Bolte Gabriele ,   on behalf of the INGER Study Group
Published: March 2023

Exposure to green space has a positive impact on health. Whether sex/gender modifies the green space–health association has so far only been studied through the use of a binary sex/gender category; however, sex/gender should be considered more comprehensively as a multidimensional concept based on theoretical approaches. We therefore explored whether sex/gender, operationalized through multiple sex/gender- and intersectionality-related covariates, modifies the green space–self-rated health association. We collected data from participants involved in the German KORA study (Cooperative Health Research in the Region of Augsburg) in 2019. Self-rated health was assessed as a one-question item. The availability of green spaces was measured subjectively as well as objectively. The multiple sex/gender- and intersectionality-related covariates were measured via self-assessment. To analyze the data, we used model-based recursive partitioning, a decision tree method that can handle complex data, considering both multiple covariates and their possible interactions. We showed that none of the covariates operationalizing an individual sex/gender self-concept led to subgroups with heterogeneous effects in the model-based tree analyses; however, we found effect heterogeneity based on covariates representing structural aspects from an intersectionality perspective, although they did not show the intersectional structuring of sex/gender dimensions. In one identified subgroup, those with a lower education level or a feeling of discrimination based on social position showed a positive green space–self-rated health association, while participants with a higher education level or no feeling of discrimination based on social position had a high level of self-rated health regardless of the availability of green spaces. Model-based recursive partitioning has the potential to detect subgroups exhibiting different exposure–outcome associations, with the possibility of integrating multiple sex/gender- and intersectionality-related covariates as potential effect modifiers. A comprehensive assessment of the relevance of sex/gender showed effect heterogeneity based on covariates representing structural aspects from an intersectionality perspective.
Lessons (Not) Learned: Chicago Death Inequities during the 1918 Influenza and COVID-19 Pandemics
Authors: Mendenhall Ruby , Shin Jong Cheol , Adibu Florence , Yago Malina Marlyn , Vandewalle Rebecca , Greenlee Andrew , Grigsby-Toussaint Diana S.
Published: March 2023

During historical and contemporary crises in the U.S., Blacks and other marginalized groups experience an increased risk for adverse health, social, and economic outcomes. These outcomes are driven by structural factors, such as poverty, racial residential segregation, and racial discrimination. These factors affect communities’ exposure to risk and ability to recover from disasters, such as pandemics. This study examines whether areas where descendants of enslaved Africans and other Blacks lived in Chicago were vulnerable to excess death during the 1918 influenza pandemic and whether these disparities persisted in the same areas during the COVID-19 pandemic. To examine disparities, demographic data and influenza and pneumonia deaths were digitized from historic weekly paper maps from the week ending on 5 October 1918 to the week ending on 16 November 1918. Census tracts were labeled predominantly Black or white if the population threshold for the group in a census tract was 40% or higher for only one group. Historic neighborhood boundaries were used to aggregate census tract data. The 1918 spatial distribution of influenza and pneumonia mortality rates and cases in Chicago was then compared to the spatial distribution of COVID-19 mortality rates and cases using publicly available datasets. The results show that during the 1918 pandemic, mortality rates in white, immigrant and Black neighborhoods near industrial areas were highest. Pneumonia mortality rates in both Black and immigrant white neighborhoods near industrial areas were approximately double the rates of neighborhoods with predominantly US-born whites. Pneumonia mortality in Black and immigrant white neighborhoods, far away from industrial areas, was also higher (40% more) than in US-born white neighborhoods. Around 100 years later, COVID-19 mortality was high in areas with high concentrations of Blacks based on zip code analysis, even though the proportion of the Black population with COVID was similar or lower than other racial and immigrant groups. These findings highlight the continued cost of racial disparities in American society in the form of avoidable high rates of Black death during pandemics.
Attachment in Couples Coping with Cancer: Associations with Observed Communication and Long-Term Health
Authors: Ramos Katherine , Leo Karena , Porter Laura S. , Romano Joan M. , Baucom Brian R. W. , Langer Shelby L.
Published: March 2023

Cancer poses a threat to well-being that may activate the attachment system and influence interpersonal dynamics, such as communication. Research indicates that avoidant and anxious attachment, as well as communication, are independently associated with poorer psychosocial well-being, yet studies examining links between attachment, communication, and long-term physical well-being are lacking. We examined (a) associations between patient and partner attachment (measured with the adult attachment scale [AAS-Revised]) and observed communication (across affect [the Relational Affective Topography System (RATS) coding system] and behavior [the Asymmetric Behavior Coding System (ABCS) coding system]) and (b) the extent to which attachment and communication independently predicted long-term physical well-being (measured by the Functional Assessment of Cancer Therapy-General Population [FACT-GP]). Participants were 134 couples [mean age 53.9 (SD = 13.4), 86.2% Caucasian, 66% of patients, 36% of partners female]. Patient participants had either breast, colorectal, or lung cancer. Couples individually completed self-report measures of attachment (baseline) and physical well-being (baseline and 4, 8, and 12 months later). At baseline, couples engaged in a 15 min videorecorded cancer-related conversation coded for communication behavior and affective expression. Patients and partners with higher anxious and avoidant attachment exhibited more negative affect and negative approach behaviors. A greater avoidant attachment was associated with less positive affective expression. Attachment insecurity and affective expression were prospectively linked with physical well-being. Findings indicate that attachment is associated with overt communication behaviors and that insecure attachment and affective expression may be risk factors for poorer health outcomes.
Ultrastructural Alterations of the Glomerular Filtration Barrier in Fish Experimentally Exposed to Perfluorooctanoic Acid
Authors: Manera Maurizio , Casciano Fabio , Giari Luisa
Published: March 2023

Per- and polyfluoroalkyl substances can be referred to as the most critical group of contaminants of emerging concern. They can accumulate in high concentration in the kidney and are known to potentially affect its function. Nonetheless, there is a lack of knowledge about their morphopathological effect on the glomerular filtration barrier. Since previous research suggests perfluorooctanoic acid (PFOA) induces glomerular protein leakage, the glomerular filtration barrier of 30 carp from the same parental stock (10 unexposed; 10 exposed to 200 ng L−1 of PFOA; and 10 exposed to 2 mg L−1 of PFOA for 56 days) was screened for possible PFOA-induced ultrastructural lesions in order to shed light on the related pathophysiology. PFOA exposure affected the glomerular filtration barrier in carp experimentally exposed to 2 mg L−1, showing ultrastructural alterations compatible with glomerulonephrosis: podocyte effacement, reduction of filtration slits and filtration slit diaphragms, basement membrane disarrangement, and occurrence of proteinaceous material in the urinary space. The results of the present research confirm the glomerular origin of the PFOA-induced protein leakage and can contribute to the mechanistic comprehension of PFOA’s impact on renal function and to the assessment of the exposure effect of environmental pollutants on animals and humans, according to the One Health approach.
Patterns of Chromosomal Variation, Homoeologous Exchange, and Their Relationship with Genomic Features in Early Generations of a Synthetic Rice Segmental Allotetraploid
Authors: Li Guo , Wu Ying , Bai Yan , Zhao Na , Jiang Yuhui , Li Ning , Lin Xiuyun , Liu Bao , Xu Chunming
Published: March 2023

Polyploidization is a driving force in plant evolution. Chromosomal variation often occurs at early generations following polyploid formation due to meiotic pairing irregularity that may compromise segregation fidelity and cause homoeologous exchange (HE). The trends of chromosomal variation and especially factors affecting HE remain to be fully deciphered. Here, by whole-genome resequencing, we performed nuanced analyses of patterns of chromosomal number variation and explored genomic features that affect HE in two early generations of a synthetic rice segmental allotetraploid. We found a wide occurrence of whole-chromosome aneuploidy and, to a lesser extent, also large segment gains/losses in both generations (S2 and S4) of the tetraploids. However, while the number of chromosome gains was similar between S2 and S4, that of losses in S4 was lower than in S2. HEs were abundant across all chromosomes in both generations and showed variable correlations with different genomic features at chromosomal and/or local scales. Contents of genes and transposable elements (TEs) were positively and negatively correlated with HE frequencies, respectively. By dissecting TEs into different classes, retrotransposons were found to be negatively correlated with HE frequency to a stronger extent than DNA transposons, whereas miniature terminal inverted elements (MITEs) showed a strong positive correlation. Local HE frequencies in the tetraploids and homologous recombination (HR) rates in diploids within 1 Mb sliding windows were significantly correlated with each other and showed similar overall distribution profiles. Nonetheless, non-concordant trends between HE and HR rates were found at distal regions in some chromosomes. At local scale, both shared and polymorphic retrotransposons between parents were negatively correlated with HE frequency; in contrast, both shared and polymorphic MITEs showed positive correlations with HE frequency. Our results shed new light on the patterns of chromosomal number variation and reveal genomic features influencing HE frequency in early generations following plant polyploidization.
Hemicyanine-Based Near-Infrared Fluorescence Off–On Probes for Imaging Intracellular and In Vivo Nitroreductase Activity
Authors: Lee Sun Hyeok , Park Chul Soon , Lee Kyung Kwan , Han Tae-Hee , Ban Hyun Seung , Lee Chang-Soo
Published: March 2023

Nitroreductase (NTR) has the ability to activate nitro group-containing prodrugs and decompose explosives; thus, the evaluation of NTR activity is specifically important in pharmaceutical and environmental areas. Numerous studies have verified effective fluorescent methods to detect and image NTR activity; however, near-infrared (NIR) fluorescence probes for biological applications are lacking. Thus, in this study, we synthesized novel NIR probes (NIR-HCy-NO2 1–3) by introducing a nitro group to the hemicyanine skeleton to obtain fluorescence images of NTR activity. Additionally, this study was also designed to propose a different water solubility and investigate the catalytic efficiency of NTR. NIR-HCy-NO2 inherently exhibited a low fluorescence background due to the interference of intramolecular charge transfer (ICT) by the nitro group. The conversion from the nitro to amine group by NTR induced a change in the absorbance spectra and lead to the intense enhancement of the fluorescence spectra. When assessing the catalytic efficiency and the limit of detection (LOD), including NTR activity imaging, it was demonstrated that NIR-HCy-NO2 1 was superior to the other two probes. Moreover, we found that NIR-HCy-NO2 1 reacted with type I mitochondrial NTR in live cell imaging. Conclusively, NIR-HCy-NO2 demonstrated a great potential for application in various NTR-related fields, including NTR activity for cell imaging in vivo.
Metabolic Composition and Quality Traits of Polygonatum cyrtonema Hua from Different Germplasms and Age Sections Based on Widely Targeted Metabolomics Analysis
Authors: Wang Qingshuang , Ban Jingjie , Cai Roudi , Zhang Xueying , Lai Chunwang , Chen Yan , Li Xiaoli , Chen Cuirong , Chen Yukun , Zhang Zihao , Lai Zhongxiong , Lin Yuling
Published: March 2023

Polygonatum rhizomes are rich in various compounds with many biological activities and are widely used in functional foods and pharmaceutical products. In order to screen for superior Polygonatum cyrtonema Hua (P. cyrtonema) germplasm and also to elucidate the nutritional and medicinal values of rhizomes, the metabolic composition and quality traits of rhizomes from different germplasms and age sections of P. cyrtonema were analysed by widely targeted metabolomics, and the molecular mechanism of triacylglycerol synthesis was explored. The results showed that the different germplasms and age sections of P. cyrtonema were rich in different nutritional and medicinal components. Of these, the broad-leaved green stem (GK) germplasm is rich in polysaccharides, alkaloids, and lipids; the pointed-leaved green stem (JL) germplasm is rich in flavonoids, steroids, and amino acids, while the pointed-leaved purple stem (JZ) germplasm contains more phenolic acids. The one-year (AT) age section is rich in polysaccharides, steroids, organic acids, and lipids; the three years (CT) age section contains more flavonoids, alkaloids, and amino acid metabolites. Lipids were significantly enriched in the broad-leaved green stem germplasm and the one-year age section. Interestingly, the highest accumulation of triacylglycerols, an important component of lipids, was also found in the GK germplasm and the AT age section. Nineteen, 14, and 13 members of the glycerol-3-phosphate acyltransferase (GPAT), lysophosphatidic acid acyltransferase (LPAT), and diacylglycerol acyltransferase (DGAT) gene families, respectively, involved in triacylglycerol synthesis were also identified. The quantitative real-time PCR (qRT-PCR) results further suggested that the differentially expressed PcDGAT1, PcDGAT2.4, PcGPAT9.1, PcLPAT2.9, and PcLPAT4.3 genes may play important roles in triacylglycerol synthesis in P. cyrtonema. Therefore, this study provides a new theoretical reference for product development and the breeding of new varieties of Polygonatum species.
Brain-Derived Major Glycoproteins Are Possible Biomarkers for Altered Metabolism of Cerebrospinal Fluid in Neurological Diseases
Authors: Hoshi Kyoka , Kanno Mayumi , Goto Aya , Ugawa Yoshikazu , Furukawa Katsutoshi , Arai Hiroyuki , Miyajima Masakazu , Takahashi Koichi , Hattori Kotaro , Kan Keiichi , Saito Takashi , Yamaguchi Yoshiki , Mitsufuji Takashi , Araki Nobuo , Hashimoto Yasuhiro
Published: March 2023

Cerebrospinal fluid (CSF) plays an important role in the homeostasis of the brain. We previously reported that CSF major glycoproteins are biosynthesized in the brain, i.e., lipocalin-type prostaglandin D2 synthase (L-PGDS) and transferrin isoforms carrying unique glycans. Although these glycoproteins are secreted from distinct cell types, their CSF levels have been found to be highly correlated with each other in cases of neurodegenerative disorders. The aim of this study was to examine these marker levels and their correlations in other neurological diseases, such as depression and schizophrenia, and disorders featuring abnormal CSF metabolism, including spontaneous intracranial hypotension (SIH) and idiopathic normal pressure hydrocephalus (iNPH). Brain-derived marker levels were found to be highly correlated with each other in the CSF of depression and schizophrenia patients. SIH is caused by CSF leakage, which is suspected to induce hypovolemia and a compensatory increase in CSF production. In SIH, the brain-derived markers were 2–3-fold higher than in other diseases, and, regardless of their diverse levels, they were found to be correlated with each other. Another abnormality of the CSF metabolism, iNPH, is possibly caused by the reduced absorption of CSF, which secondarily induces CSF accumulation in the ventricle; the excess CSF compresses the brain’s parenchyma to induce dementia. One potential treatment is a “shunt operation” to bypass excess CSF from the ventricles to the peritoneal cavity, leading to the attenuation of dementia. After the shunt operation, marker levels began to increase within a week and then further increased by 2–2.5-fold at three, six, and twelve months post-operation, at which point symptoms had gradually attenuated. Notably, the marker levels were found to be correlated with each other in the post-operative period. In conclusion, the brain-derived major glycoprotein markers were highly correlated in the CSF of patients with different neurological diseases, and their correlations were maintained even after surgical intervention. These results suggest that brain-derived proteins could be biomarkers of CSF production.