bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of precipitation variability and conservation tillage on soil moisture, yield and quality of silage maize
Authors: Niu Lili , Qin Wangfei , You Yongliang , Mo Qishun , Pan Jie , Tian Lihua , Xu Guang , Chen Chao , Li Zhou
Published: July 2023

To cope with the change of precipitation pattern, we explored the effects of conservation tillage and precipitation variability on soil moisture status and silage maize productivity, seeking solutions to improve the yield and quality of maize in the karst areas of China. In this study, precipitation variability and conservation tillage were jointly analyzed by precipitation levels (normal precipitation, 30% lower and 30% greater than normal precipitation), tillage methods (conventional tillage and no-tillage) and cover crop mulching (mulching and no mulching). It was found that no tillage (NT) increased the maize yield by 12.85% compared with the conventional tillage (CT) treatments. Above-normal precipitation treatments did not significantly increase biomass and soil water content across different fertility stages, but below-normal precipitation treatments reduced maize yield, crude protein (CP) content and soil water content at all soil levels. CT treatment significantly increased soil water content in the tillage layer (0–40 cm), but NT treatment significantly increased dry matter yield, plant height and CP content at harvest by 10.99, 3.41, 5.62%. Cover crop mulching significantly increased maize yield by 8.26% and soil moisture content at the seedling stage in 0–40 cm soil by 2.76 and 2.52%. The three-way interaction effects involving precipitation levels, tillage methods and cover crop mulching were significant for maize yield, CP content and soil water content (p < 0.05). In addition, this study found that soil water content was positively correlated with crude protein content of maize (p < 0.05). In conclusion, this study concluded that a combination of NT and cover crop mulching should be used to mitigate maize production risks caused by precipitation variability in the karst areas.
Combining deficit irrigation and nutrient amendment enhances the water productivity of tomato (Solanum lycopersicum L.) in the tropics
Authors: Amankwaa-Yeboah Patricia , Aruna Akoriko Fati , Amponsah William , Yeboah Stephen , Brempong Mavis Badu , Keteku Agbesi K.
Published: July 2023

Water availability for irrigation farming is one of the greatest challenges associated with the increasing spatio-temporal effects of climate change and variability on tomato production, especially in tropical regions. This study was conducted to demonstrate the combined effect of irrigation and nutrient management as a water-saving strategy to maximize nutrient and water productivity in tomato production. The research was conducted in a screen house at the CSIR-Crops Research Institute (CSIR-CRI), Kumasi, Ghana using the split-plot design in the 2020 and 2021 cropping seasons. The main plot consisted of a single full irrigation at 100% recommended crop water requirement and two deficit irrigation levels (75 and 50% of the crop water requirement). The subplots consisted of two nutrient amendments (inorganic fertilizer and organic compost) and a control (without any nutrient amendment). Data from the research was used to parameterize the DSSAT CCROPGRO model to simulate the interactive effect of irrigation and nutrient management on the yield of tomatoes. Plant height and stem girth did not have a specific influence on tomato yield, but the number of branches had a positive effect on tomato yield. The combined use of inorganic fertilizer and full irrigation was found to improve tomato yield up to 7691.4 and 9009.9 kg/ha whereas treatment with no fertilizer application at 50% deficit irrigation recorded the lowest tomato yield of 1423.9 and 1739.2 kg/ha in 2020 and 2021, respectively. For the two deficit irrigations (50 and 75% ETc), organic compost produced the highest tomato yield. Deficit irrigation recorded higher crop water productivity (CWP) compared to full irrigation. At 50% deficit irrigation, organic compost recorded the highest CWP of 4.54 kg/m3in 2020 while inorganic fertilizer recorded the highest CWP of 5.52 kg/m3in 2021. No fertilizer at full irrigation recorded the lowest CWP of 1.37 and 1.67 kg/m3in 2020 and 2021, respectively. This study has revealed that deficit irrigation with organic compost has the same effect on yield and water productivity as full irrigation with inorganic fertilizer. The strong agreement observed between the measured and simulated yields under the different irrigation and nutrient management shows that the DSSAT CROPGRO tomato model can be used to simulate tomato fruit yield under future climate scenarios. However, the general overestimation of the measured tomato yield shows the limitations of the model to simulate the real-world complexity of cropping systems under controlled conditions. This calls for more research into crop system modeling in controlled environment agriculture.
Exploring Subjective Happiness, Life Satisfaction, and Sustainable Luxury Consumption in China and Japan Amidst the COVID-19 Pandemic
Authors: Lee Kyung-Tae , Furukawa Hiroyasu
Published: July 2023

The COVID-19 pandemic has significantly impacted societies worldwide, leading to challenges in ensuring well-being and sustainability. In this context, it is essential to better understand consumer consciousness of pro-environmental products from the perspective of well-being. Therefore, our cross-national investigation aimed to explore the relationship between subjective well-being (SWB) and sustainable luxury consumption during the pandemic. We analyzed data from 800 respondents in China and Japan during the pandemic. Our findings, obtained through partial least squares structural equation modeling, reveal novel insights. First, SWB positively influences pro-environmental behavioral intentions, even in luxury fashion consumption. This highlights the potential for individuals to make sustainable choices, while indulging in luxury products. Additionally, we observed variations in the impact of subjective happiness and life satisfaction on luxury consumption between China and Japan. Specifically, the influence of subjective happiness was more pronounced in China than in Japan, indicating cultural distinctions in the relationship between well-being and luxury consumption. Moreover, our study identifies consumer novelty seeking as a mediator linking SWB to sustainable luxury consumption. This discovery provides a further understanding of the relationships explored in our study. These findings deepen our understanding of the interplay between well-being and sustainable luxury consumption, thereby informing strategies to promote pro-environmental behaviors in the luxury fashion sector.
Effect of Exposure to Boarding Schooling on Pregnancy among School-Going Teenagers: A Retrospective Case-Control Study in Eastern Province, Zambia
Authors: Tembo Nasson N. , Jacobs Choolwe , Maliko Catherine N. , Musonda Patrick
Published: July 2023

Cases of teenage pregnancy remain high in Eastern Province of Zambia—contributing to health, economic, and social problems. This study sought to determine the effect of exposure to boarding schooling on pregnancy among school-going teenagers, taking into account individual and school characteristics. This was a retrospective case-control study involving 261 cases and 522 controls obtained from nine boarding and nine day secondary schools, between 2019 and 2021. STATA 16.1 MP was used for all statistical analyses at a 5% level of significance. Unadjusted and adjusted effects were obtained using logistic regression analysis—taking into account 18 school clusters. Cases had a mean age of 17.4 years (±1.14 years), controls were 16.1 years (±1.71 years) old on average, and 315 (40%) of the participants were exposed to boarding schooling. The unadjusted odds of pregnancy in the exposed and unexposed (day scholars) girls were 0.25 and 0.73, respectively (cOR = 0.34, CI: 0.24–0.48, p < 0.0001). Adjusting for other characteristics, teenage girls in boarding schools had 60% lower odds of pregnancy (aOR = 0.40, CI = 0.16–1.00, p = 0.049). In light of this evidence, enrollment of teenage girls in boarding schools is encouraged to help mitigate teen pregnancy in the province. Additionally, a multicenter prospective study is recommended.
Study on the Cooling Effect of Double-Layer Spray Greenhouse
Authors: Xu Jihang , Bai Weitao , Wang Jian , Mu Zhihui , Sun Weizhen , Dong Boda , Song Kai , Yang Yalan , Guo Shirong , Shu Sheng , Wang Yu
Published: July 2023

Greenhouses provide suitable environmental conditions for plant growth. Double-layer plastic greenhouses are often used in many regions to ensure normal crop growth during winter since single-layer plastic greenhouses have poor insulation. However, during summer, the high insulation of double-layer plastic greenhouses, combined with excessive external solar radiation, can cause high temperatures inside the greenhouse that are not suitable for plant growth and require cooling. In this study, we propose a double-layer spray greenhouse using a high-pressure spraying system that is placed inside the double film that allows for additional cooling capacity during the summer in order to sustain plant growth. A greenhouse platform test was set up to investigate the optimum operating conditions for the nozzles and to explore changes in greenhouse microclimate under different nozzle operating conditions. The results show that (1) the cooling rate increases with increasing water supply pressure, nozzle diameter and spraying time, and the humidification rate is consistent with the change in the rate of cooling. (2) The optimal condition for cooling in this experiment is achieved with a 120° double nozzle with a nozzle diameter of 0.30 mm, a water supply pressure of 6 MPa, and a spraying time of 15 min, which can reduce the temperature by up to 5.36 °C and serve as a reference for the summer cooling of the double-layer greenhouse.
Mathematical Models to Predict Dry Matter Intake and Milk Production by Dairy Cows Managed under Tropical Conditions
Authors: Gurgel Antonio Leandro Chaves , Santos Geraldo Tadeu dos , Ítavo Luís Carlos Vinhas , Ítavo Camila Celeste Brandão Ferreira , Difante Gelson dos Santos , Dias Alexandre Menezes , Longhini Vanessa Zirondi , Dias-Silva Tairon Pannunzio , de Araújo Marcos Jácome , Neto João Virgínio Emerenciano , Fernandes Patrick Bezerra , Chay-Canul Alfonso Juventino
Published: July 2023

This study aimed to create an equation to predict dry matter intake (DMI) and milk production and N-ureic in the milk of dairy cows managed in tropical conditions in Brazil. We used 113 observations from three experiments using lactating Jersey, Girolando, and Holstein cows. The goodness of fit of the developed equations was evaluated using the coefficients of determination (r2) and root mean square error (RMSE). There was a positive correlation between body weight and milk yield (MY) of r = 0.73. The equation considered DMI to be the most important variable to estimate the MY (r2 = 0.65). Four equations were adjusted to estimate the DMI, where, by a stepwise procedure, the first variable included in the equation was the neutral detergent fibre intake, which explained 92% of the DMI of the cows. However, when the variables BW, MY, and milk fat were included in the equation, there was a reduction of 0.06 in RMSE and an increase in precision (r2 = 0.94). The nutrient intake, milk production, and characteristics prediction equations present satisfactory precision and accuracy for dairy cows managed in tropical conditions in Brazil.
Measuring Pathogenic Soil Fungi That Cause Sclerotinia Rot of Panax ginseng Using Real-Time Fluorescence Quantitative PCR
Authors: Feng Shi , Zhang Cong , Wang Xue , Chen Changqing , Lu Baohui , Gao Jie
Published: July 2023

Sclerotinia ginseng is the primary pathogenic fungus responsible for Sclerotinia rot of ginseng, which significantly reduces plant yield and quality. The density of sclerotia in the soil is closely associated with rot incidence and severity. Whole genome sequencing was conducted to obtain fungal frame maps. The specific primers, q2001F/q2001R, were screened out by pan-genomic analysis using the NCBI database. Recombinant plasmids containing amplicons obtained with this primer set were used as standard plasmids to construct a real-time fluorescence quantitative PCR system. The relationships between the cycle threshold (Ct) values and the soil sclerotium densities were determined by real-time PCR. Real-time PCR had a detection limit of 1.5 × 10−2 g·kg−1 soil for Sclerotinia rot causing fungal mycelium, and the relationship between the density of S. ginseng mycelium n (g·g−1 soil) and the Ct value was n = 10(40.048 − Ct)/6.9541. The detection limit of real-time PCR for measuring soil sclerotia was 3.8 × 10−5 g·g−1 soil, suggesting a sensitivity 100 times that of conventional PCR. The relationship between the sclerotium density n (g·g−1 soil) and the Ct value was n = 10(18.351 − Ct)/7.0914. Compared with the conventional PCR method, the fluorescent quantitative PCR method could detect the population of Sclerotinia spp. in soil more efficiently, accurately, and sensitively.
Identification of Water Layer Presence in Paddy Fields Using UAV-Based Visible and Thermal Infrared Imagery
Authors: Wei Guangfei , Chen Huifang , Lin En , Hu Xuhua , Xie Hengwang , Cui Yuanlai , Luo Yufeng
Published: July 2023

The accurate identification of the water layer condition of paddy fields is a prerequisite for precise water management of paddy fields, which is important for the water-saving irrigation of rice. Until now, the study of unmanned aerial vehicle (UAV) remote sensing data to monitor the moisture condition of field crops has mostly focused on dry crops, and research on the water status of paddy fields has been relatively limited. In this study, visible and thermal infrared images of paddy fields at key growth stages were acquired using a UAV remote sensing platform, and three model input variables were constructed by extracting the color features and temperature features of each field, while K-nearest neighbor (KNN), support vector machine (SVM), random forest (RF), and logistic regression (LR) analysis methods were applied to establish a model for identifying the water layer presence in paddy fields. The results showed that KNN, SVM, and RF performed well in recognizing the presence of water layers in paddy fields; KNN had the best recognition accuracy (89.29%) via algorithm comparison and parameter preference. In terms of model input variables, using multisource remote sensing data led to better results than using thermal or visible images alone, and thermal data was more effective than visible data for identifying the water layer status of rice fields. This study provides a new paradigm for monitoring the water status of rice fields, which will be key to the precision irrigation of paddy fields in large regions in the future.
Effects on Soil Chemical Properties and Carbon Stock Two Years after Compost Application in a Hedgerow Olive Grove
Authors: Alexandre Carlos A. , Bajouco Rui , Leal Jacqueline D. S. , Peça José O. , Dias António B.
Published: July 2023

Soil amendments with composted organic materials are recommended to increase soil organic matter (SOM) and promote soil fertility. Growing areas of hedged olive groves in the southern Iberia peninsula generate huge amounts of olive leaves, and their potential as an organic soil amendment is not fully studied. An experimental field trial in a hedged olive grove (“Cobrançosa”) was set up near Portalegre, Portugal, to test a compost of olive leaves plus sheep manure (with a ratio of 2:1) when applied in a row at the soil’s surface. Nominal rates of zero, 2.5, and 5.0 kg m−2 (T0, T1, and T2, respectively) were applied in a complete randomized block setup (three treatments, three replicas, and nine plots), and soil properties of layers between 0–5, 5–15, and 15–30 cm were annually monitored. More expressive results occurred in the soil layer 0–5 cm, and with the dosage T2. After one year, there were significant increases in the total N, carbon of the particulate organic matter, permanganate oxidizable carbon (POX-C), extractable phosphorus, and zinc. After two years, there was 16% more soil organic carbon (SOC), an absolute increase of 0.5 in pHKCl, 1.9 times more extractable phosphorus, and ten times more zinc. The soil’s C-stock in the 0–30 cm layer, after two years of T1 and T2 dosages, was 0.11 and 0.35 kg m−2 (~3 and ~9%, respectively), which was higher than with T0. POX-C was the most sensitive SOM-related indicator, showing increases of up to 30 cm deep after one year. This compost improved soil fertility but should be monitored over longer periods of time.
Accuracy of Estimated Crop Evapotranspiration Using Locally Developed Crop Coefficients against Satellite-Derived Crop Evapotranspiration in a Semiarid Climate
Authors: Djaman Koffi , Mohammed Ali T. , Koudahe Komlan
Published: July 2023

Actual crop evapotranspiration (ETa) is measured or estimated using different methods, and its accuracy is critical for water management under precision agriculture. The objective of this study was to compare maize ETa estimated by the two-step approach using a locally developed crop coefficient curve with satellite-retrieved evapotranspiration by six models incorporated in the OpenET to identify the best evapotranspiration estimation alternatives to the two-step approach for water management in northern New Mexico. Maize (Zea mays L.) was planted at the NMSU Agricultural Science Center at Farmington from 2017 to 2022 and uniformly managed across years. Water management in plants was based on maize’s actual evapotranspiration estimated as the product of the reference evapotranspiration and the local crop coefficient, which is described as a third-order polynomial function of the accumulated heat units by maize plants. For the same growing seasons, maize ETa was retrieved from satellite, and was estimated by six models listed within the OpenET from 2017 to 2022. The results show that maize daily ETa was consistently smaller when measured by SIMS and PT-JPL during maize initial and actively growing stages, while ETc(kc), SIMS and eeMETRIC showed similar maize daily ETa during maize full canopy development and mid-season, and which overcome the evapotranspiration estimated by DisALEXI, PT-JPL, geeSEBAL, and SSBop. ETc(kc) drastically dropped and became the lowest value among all ETa estimation models after the first fall snow or the first killing frost. Regarding the seasonal average, all six models included in OpenET showed smaller maize evapotranspiration. Maize seasonal evapotranspiration varied from 589.7 to 683.2 mm. eeMETRIC compares most similarly to the ETc(kc) model, followed by SIMS, with percent errors of 2.58 and 7.74% on a daily basis and 2.43 and 7.88% on a seasonal basis, with the lowest MBE and RMSE values, respectively, and could be used as an alternative for maize actual daily evapotranspiration for water management in northern New Mexico. The results of this study could be used by water managers and crop growers to improve water management in the Four Corners region, using eeMETRIC for crop water use to improve water management and conservation under sustainable agriculture.
Methodologies for Water Accounting at the Collective Irrigation System Scale Aiming at Optimizing Water Productivity
Authors: Ferreira Antónia , Rolim João , Paredes Paula , Cameira Maria do Rosário
Published: July 2023

To improve water use efficiency and productivity, particularly in irrigated areas, reliable water accounting methodologies are essential, as they provide information on the status and trends in irrigation water availability/supply and consumption/demand. At the collective irrigation system level, irrigation water accounting (IWA) relies on the quantification of water fluxes from the diversion point to the plants, at both the conveyance and distribution network and the irrigated field level. Direct measurement is the most accurate method for IWA, but in most cases, there is limited metering of irrigation water despite the increasing pressure on both groundwater and surface water resources, hindering the water accounting procedures. However, various methodologies, tools, and indicators have been developed to estimate the IWA components, depending on the scale and the level of detail being considered. Another setback for the wide implementation of IWA is the vast terminology used in the literature for different scales and levels of application. Thus, the main objectives of this review, which focuses on IWA for collective irrigation services, are to (i) demonstrate the importance of IWA by showing its relationship with water productivity and water use efficiency; (ii) clarify the concepts and terminology related to IWA; and (iii) provide an overview of various approaches to obtain reliable data for the IWA, on the demand side, both at the distribution network and on-farm systems. From the review, it can be concluded that there is a need for reliable IWA, which provides a common information base for all stakeholders. Future work could include the development of user-friendly tools and methodologies to reduce the bridge between the technology available to collect and process the information on the various water accounting components and its effective use by stakeholders.
Effect of Different Ratios of Red and Blue Light on Maximum Stomatal Conductance and Response Rate of Cucumber Seedling Leaves
Authors: Li Xue , Zhao Shiwen , Lin Aiyu , Yang Yuanyuan , Zhang Guanzhi , Xu Peng , Wu Yongjun , Yang Zhenchao
Published: July 2023

Light can regulate leaf stomatal development and movement, but the effects of different red-to-blue light mass ratios on leaf stomatal morphology and openness are not fully understood. In this trial, five different red-to-blue light (R:B) ratio treatments were used to study the changes in morphology, photosynthesis, and stomatal-related indexes of cucumber seedlings under fixed light intensity (200 μmol·m−2·s−1). The results showed that the thickness of spongy tissue and stomatal size (SZ) of cucumber seedling leaves decreased, and the photosynthetic potential, stomatal density (SD), maximum stomatal conductance and stomatal responsiveness increased with decreasing R:B content. The experimental results showed that when R:B = is 1:9, cucumber seedlings had the greatest stomatal density and the fastest response rate, and the stomatal opening rate was accelerated with the increase in the proportion of blue light; when R:B = is 3:7, the stomatal conductance was the greatest and the net photosynthetic rate was the highest. This trial provides some implications for changing plant light quality and thus affecting stomatal development and movement.
Antifungal Activity of Streptomyces hygroscopicus JY-22 against Alternaria alternata and Its Potential Application as a Biopesticide to Control Tobacco Brown Spot
Authors: Cai Lin , Zhang Hongbao , Deng Yongjie , Tian Weiqiang , Fan Guangjin , Sun Xianchao
Published: July 2023

Tobacco brown spot caused by Alternaria alternata is a devastating fungal disease endangering plant production. To effectively control brown spot disease, an antagonistic Actinomyces strain, Streptomyces hygroscopicus JY-22, was isolated from rhizospheric soil, which remarkably restrains the growth of phytopathogenic fungus Alternaria alternata. This antagonistic strain and its culture filtrate showed significant antifungal activity against Alternaria alternata. Firstly, the confrontation culture method of strains JY-22 and Alternaria alternata showed that Streptomyces hygroscopicus JY-22 had noticeable antifungal activity against Alternaria alternata, including inhibition of mycelial growth and mycelial morphological changes, compared with the control group. Furthermore, the culture filtrate of Streptomyces hygroscopicus JY-22 showed substantial inhibition of the mycelial growth and spore germination of Alternaria alternata in a dose-dependent manner. Additional studies revealed that these antifungal actions were mainly related to membrane-active mechanisms that increased membrane permeability and damaged the cell membrane, leading to changes in certain cytoplasmic properties, such as extracellular conductivity and ergosterol, MDA and soluble protein content. In detached-leaf and field experiments, foliar spraying with culture filtrate resulted in smaller lesions and a lower disease index than the control group. Taken together, these results suggest that Streptomyces hygroscopicus JY-22 and its culture filtrate have the potential to be a safe biopesticide for the bio-control of tobacco brown spot.
Evaluating Critical Nitrogen Dilution Curves for Assessing Maize Nitrogen Status across the US Midwest
Authors: Shao Hui , Miao Yuxin , Fernández Fabián G. , Kitchen Newell R. , Ransom Curtis J. , Camberato James J. , Carter Paul R. , Ferguson Richard B. , Franzen David W. , Laboski Carrie A. M. , Nafziger Emerson D. , Sawyer John E. , Shanahan John F.
Published: July 2023

Plant N concentration (PNC) has been commonly used to guide farmers in assessing maize (Zea mays L.) N status and making in-season N fertilization decisions. However, PNC varies based on the development stage. Therefore, a relationship between biomass and N concentration is needed (i.e., critical N dilution curve; CNDC) to better understand when plants are N deficient. A few CNDCs have been developed and used for plant N status diagnoses but have not been tested in the US Midwest. The objective of this study was to evaluate under highly diverse soil and weather conditions in the US Midwest the performance of CNDCs developed in France and China for assessing maize N status. Maize N rate response trials were conducted across eight US Midwest states over three years. This analysis utilized plant and soil measurements at V9 and VT development stages and final grain yield. Results showed that the French CNDC (y = 34.0x−0.37, where y is critical PNC, and x is aboveground biomass) was better with a 91% N status classification accuracy compared to only 62% with the Chinese CNDC (y = 36.5x−0.48). The N nutrition index (NNI), which is the quotient of the measured PNC and the calculated critical N concentration (Nc) based on the French CNDC was significantly related to soil nitrate-N content (R2 = 0.38–0.56). Relative grain yield on average reached a plateau at NNI values of 1.36 at V9 and 1.21 at VT but for individual sites ranging from 0.80 to 1.41 at V9 and from 0.62 to 1.75 at VT. The NNI threshold values or ranges optimal for crop biomass production may not be optimal for grain yield production. It is concluded that the CNDC developed in France is suitable as a general diagnostic tool for assessing maize N status in US Midwest. However, the threshold values of NNI for diagnosing maize N status and guiding N applications vary significantly across the region, making it challenging to guide specific on-farm N management. More studies are needed to determine how to effectively use CNDC to make in-season N recommendations in the US Midwest.
Quantitative and Qualitative Traits of Duckweed (Lemna minor) Produced on Growth Media with Pig Slurry
Authors: Sońta Marcin , Więcek Justyna , Szara Ewa , Rekiel Anna , Zalewska Anna , Batorska Martyna
Published: July 2023

Duckweed is a plant with high phytoremediation abilities, which is why it is used in the process of cleaning the aquatic environment. The present study aimed to determine the effect of various concentrations of pig slurry added to the growth media used to produce duckweed (Lemna minor) (laboratory Warsaw University of Life Sciences—SGGW) (experimental groups 1–9, pig slurry concentration (%): 1—2.00, 2—1.50, 3—1.00, 4—0.75, 5—0.50, 6—0.25, 7—0.12, 8—0.06, 9—0.03, control group 0—0.00). The contents of nutrients in the growth media could be classified as high (gr. 1–3), optimal (gr. 4–6), and deficient (gr. 7–9). Analyses were conducted for duckweed yield and growth medium parameters (pig slurry concentration, pH, salinity, temperature, TDS, and EC) on days 0, 10, 20, and 30 of the experiment. No growth or poor growth of duckweed were noted in groups 1, 6–9, and 0. In turn, satisfactory yields of duckweed green mass were recorded in groups 3–5, which allowed choosing them for further observations and analyses, including proximate composition (including protein content); contents of Ca, Mg, K, Na, Zn, Cu, Cd, Pb, Al, Cr, and α-tocopherol; and carotenoids—β-carotene, α-carotene, violaxanthin, zeaxanthin, lutein, amino acids, fatty acids as well as N-NH4 and N-NO3. The plant material had an acceptable proximate composition and nutritionally safe analyzed component contents. Appropriate, stable growth medium conditions allowed the production of satisfactory duckweed yields. The study results allowed us to conclude that it is feasible to obtain feed material meeting basic quality standards by maintaining a closed circuit of duckweed culture, and use in the agricultural environment is possible through harnessing pig slurry for its production and ensuring its optimal growth conditions.
Non-Invasive Techniques Reveal Heifer Response to Fescue Endophyte Type in Grazing Studies
Authors: Poudel Sanjok , Fike John H. , Wright Lee , Pent Gabriel J.
Published: July 2023

Cattle grazing tall fescue (Schedonorus arundinaceous) infected with wild-type endophytes (WE) leads to a syndrome commonly known as fescue toxicosis. Replacing WE tall fescue with a novel endophyte-infected (NE) tall fescue can mitigate this problem but adoption of this technology has been limited. This study measured and determined the physiological and behavioral responses of heifers that grazed either WE or NE tall fescue, utilizing relatively non-invasive techniques including hair cortisol, thermography (for extremity temperatures), small loggers for intravaginal temperature, and remote observation of in-field behavior. Heifers that grazed WE had greater (p < 0.0001) hair cortisol levels, lower extremity temperatures (p ≤ 0.0075), and 0.3–0.9 °C greater (p ≤ 0.02) intravaginal temperatures (particularly during the daytime) than heifers that grazed NE. From 1200 h–1700 h each day, heifers on WE pastures spent 1.5 more (p = 0.0003) hours standing up and 0.9 fewer (p = 0.0402) hours lying down than heifers on NE pastures. Differences (p = 0.0160) in ADG were small (0.1 kg d−1) and were only observed in the first year of these 8-week studies. However, even in the mild environment of the study site, grazing NE tall fescue provided clear welfare benefits as evidenced by heifer behavioral changes, temperature differentials, and hair cortisol levels. This study underscores the potential utility of non-invasive techniques, such as thermographic imaging and hair cortisol analysis, for evaluating animal responses to stress in extensive grazing systems.
Relationship between Consumers’ Perceptions about Goat Kid Meat and Meat Sensory Appraisal
Authors: Alcalde María J. , Ripoll Guillermo , Campo María M. , Horcada Alberto , Panea Begoña
Published: July 2023

The goat meat preferred by consumers in Spain comes from suckling goat kids, slaughtered at a live weight of 8–10 kg. However, consumption of this meat is very uncommon, so it is necessary to show its value. To achieve this, we planned to investigate consumers’ perceptions about goat kid meat and to study whether their perceptions are related to their sensory appraisal of the meat, measured by the mean of the consumers’ home tests. The experiment was conducted with 30 volunteer families (from two cities with different consumption patterns), who were surveyed regarding their sociodemographic parameters, purchasing and eating habits, and the importance of the meat’s attributes. As a result, four clusters were obtained, which were labeled “negative,” “idealistic,” “positive,” and “concerned about fat content”. The parameters of the animal production system were valued differently by the clusters. Meat tenderness, taste, and amount of fat were considered very decisive factors by most respondents. When the goat kid meat was valued, tenderness was considered more important than taste among older people (“negative” cluster), whereas there was not so much difference between the appraisal of all parameters for the other three clusters. We conclude that it is necessary to improve the information received by consumers about goat production systems and meat quality parameters. There is certainly potential for creating new markets, especially targeted toward young consumers and considering specific strategies for the different groups of consumers, depending on the region and habits of consumption.
Adsorption/Desorption of Cationic-Hydrophobic Peptides on Zwitterionic Lipid Bilayer Is Associated with the Possibility of Proton Transfer
Authors: Pašalić Lea , Jakas Andreja , Pem Barbara , Bakarić Danijela
Published: July 2023

Cell-penetrating peptides (CPPs) are short peptides built up from dominantly cationic and hydrophobic amino acid residues with a distinguished ability to pass through the cell membrane. Due to the possibility of linking and delivering the appropriate cargo at the desired location, CPPs are considered an economic and less invasive alternative to antibiotics. Besides knowing that their membrane passage mechanism is a complex function of CPP chemical composition, the ionic strength of the solution, and the membrane composition, all other details on how they penetrate cell membranes are rather vague. The aim of this study is to elucidate the ad(de)sorption of arginine-/lysine- and phenylalanine-rich peptides on a lipid membrane composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) lipids. DSC and temperature-dependent UV-Vis measurements confirmed the impact of the adsorbed peptides on thermotropic properties of DPPC, but in an inconclusive way. On the other hand, FTIR spectra acquired at 30 °C and 50 °C (when DPPC lipids are found in the gel and fluid phase, respectively) unambiguously confirmed the proton transfer between particular titratable functional groups of R5F2/K5F2 that highly depend on their immediate surroundings (DPPC or a phosphate buffer). Molecular dynamic simulations showed that both peptides may adsorb onto the bilayer, but K5F2 desorbs more easily and favors the solvent, while R5F2 remains attached. The results obtained in this work highlight the importance of proton transfer in the design of CPPs with their desired cargo, as its charge and composition dictates the possibility of entering the cell.
Long-Term Use of Amoxicillin Is Associated with Changes in Gene Expression and DNA Methylation in Patients with Low Back Pain and Modic Changes
Authors: Vigeland Maria Dehli , Flåm Siri Tennebø , Vigeland Magnus Dehli , Espeland Ansgar , Zucknick Manuela , Wigemyr Monica , Bråten Lars Christian Haugli , Gjefsen Elisabeth , Zwart John-Anker , Storheim Kjersti , Pedersen Linda Margareth , Selmer Kaja , Lie Benedicte Alexandra , Gervin Kristina ,   The AIM Study Group
Published: July 2023

Long-term antibiotics are prescribed for a variety of medical conditions, recently including low back pain with Modic changes. The molecular impact of such treatment is unknown. We conducted longitudinal transcriptome and epigenome analyses in patients (n = 100) receiving amoxicillin treatment or placebo for 100 days in the Antibiotics in Modic Changes (AIM) study. Gene expression and DNA methylation were investigated at a genome-wide level at screening, after 100 days of treatment, and at one-year follow-up. We identified intra-individual longitudinal changes in gene expression and DNA methylation in patients receiving amoxicillin, while few changes were observed in patients receiving placebo. After 100 days of amoxicillin treatment, 28 genes were significantly differentially expressed, including the downregulation of 19 immunoglobulin genes. At one-year follow-up, the expression levels were still not completely restored. The significant changes in DNA methylation (n = 4548 CpGs) were mainly increased methylation levels between 100 days and one-year follow-up. Hence, the effects on gene expression occurred predominantly during treatment, while the effects on DNA methylation occurred after treatment. In conclusion, unrecognized side effects of long-term amoxicillin treatment were revealed, as alterations were observed in both gene expression and DNA methylation that lasted long after the end of treatment.
Do or Don’t: Results of a Multinational Survey on Antibiotic Prophylaxis in Urodynamics
Authors: Stangl Fabian P. , Schneidewind Laila , Wagenlehner Florian M. , Schultz-Lampel Daniela , Baeßler Kaven , Naumann Gert , Schönburg Sandra , Anheuser Petra , Winkelhog-Gran Susanne , Saar Matthias , Hüsch Tanja , Kranz Jennifer
Published: July 2023

Antibiotic prophylaxis contributes substantially to the increase in antibiotic resistance rates worldwide. This investigation aims to assess the current standard of practice in using antibiotic prophylaxis for urodynamics (UDS) and identify barriers to guideline adherence. An online survey using a 22-item questionnaire designed according to the Checklist for Reporting Results of Internet E-Surveys (CHERRIES) was circulated among urologists and gynecologists in Austria, Germany, and Switzerland between September 2021 and March 2022. A total of 105 questionnaires were eligible for analysis. Out of 105 completed surveys, most responders (n = 99, 94%) regularly perform dipstick urine analysis prior to urodynamics, but do not perform a urine culture (n = 68, 65%). Ninety-eight (93%) participants refrain from using antibiotic prophylaxis, and sixty-eight (65%) use prophylaxis if complicating factors exist. If asymptomatic bacteriuria is present, approximately 54 (52%) participants omit UDS and reschedule the procedure until antimicrobial susceptibility testing is available. Seventy-eight (78%) participants do not have a standard procedure for antibiotic prophylaxis in their department. Part of the strategy against the development of bacterial resistance is the optimized use of antibiotics, including antibiotic prophylaxis in urodynamics. Establishing a standard procedure is necessary and purposeful to harmonize both aspects in the field of urological diagnostics.
Benzimidazole-Triazole Hybrids as Antimicrobial and Antiviral Agents: A Systematic Review
Authors: Marinescu Maria
Published: July 2023

Bacterial infections have attracted the attention of researchers in recent decades, especially due to the special problems they have faced, such as their increasing diversity and resistance to antibiotic treatment. The emergence and development of the SARS-CoV-2 infection stimulated even more research to find new structures with antimicrobial and antiviral properties. Among the heterocyclic compounds with remarkable therapeutic properties, benzimidazoles, and triazoles stand out, possessing antimicrobial, antiviral, antitumor, anti-Alzheimer, anti-inflammatory, analgesic, antidiabetic, or anti-ulcer activities. In addition, the literature of the last decade reports benzimidazole-triazole hybrids with improved biological properties compared to the properties of simple mono-heterocyclic compounds. This review aims to provide an update on the synthesis methods of these hybrids, along with their antimicrobial and antiviral activities, as well as the structure–activity relationship reported in the literature. It was found that the presence of certain groups grafted onto the benzimidazole and/or triazole nuclei (-F, -Cl, -Br, -CF3, -NO2, -CN, -CHO, -OH, OCH3, COOCH3), as well as the presence of some heterocycles (pyridine, pyrimidine, thiazole, indole, isoxazole, thiadiazole, coumarin) increases the antimicrobial activity of benzimidazole-triazole hybrids. Also, the presence of the oxygen or sulfur atom in the bridge connecting the benzimidazole and triazole rings generally increases the antimicrobial activity of the hybrids. The literature mentions only benzimidazole-1,2,3-triazole hybrids with antiviral properties. Both for antimicrobial and antiviral hybrids, the presence of an additional triazole ring increases their biological activity, which is in agreement with the three-dimensional binding mode of compounds. This review summarizes the advances of benzimidazole triazole derivatives as potential antimicrobial and antiviral agents covering articles published from 2000 to 2023.
A Systematic Review and Meta-Analysis of Systemic and Local Antibiotic Therapy in the Surgical Treatment of Peri-Implantitis
Authors: Baus-Domínguez María , Bakkali Sara , Hermida-Cabrera Paula , Serrera-Figallo María-Angeles , Gutiérrez-Pérez José-Luis , Torres-Lagares Daniel
Published: July 2023

(1) Background: Given the existing controversy regarding the use of antibiotics in the treatment of peri-implantitis, this systematic review and meta-analysis aim to ascertain how beneficial the role of systemic and local antibiotics is in peri-implant surgical therapy, considering the harmful effects that they represent and the abuse of antibiotics in terms of global health. (2) Methods: To determine the therapeutic efficacy of the administration of antibiotics in the surgical treatment of peri-implantitis in terms of probing pocket depth (PPD) and bleeding on probing (BoP), electronic and manual bibliographic searches were carried out in the Embase and PubMed databases, collecting data that related to before and after treatment. (3) Results: The adjunctive use of local antibiotics provides significant improvements in PPD (MD = 1.29; 95% CI: 0.56 to 2.02; p ≤ 0.0006; I2 = 0%) when compared with surgical treatment alone. No significant differences were found in the other subgroup; that is, the use of systemic antibiotics did not significantly improve PPD changes in the surgical treatment of peri-implantitis (MD = 0.40; 95% CI: −0.15 to 0.95; p = 0.15; I2 = 0). (4) Conclusions: The use of local antibiotics in the surgical treatment of peri-implantitis seems to offer treatment improvements in terms of PPD and BoP, unlike that observed with the use of systemic antibiotics. However, these results should be taken with caution as they also depend on the type of surgical technique used, whether regenerative or resective. More research is needed on this topic to understand the role of local and systemic antibiotics in the treatment of peri-implantitis.
Olive Oil Phenolic Compounds’ Activity against Age-Associated Cognitive Decline: Clinical and Experimental Evidence
Authors: Boronat Anna , Serreli Gabriele , Rodríguez-Morató Jose , Deiana Monica , de la Torre Rafael
Published: July 2023

Epidemiological studies have shown that consuming olive oil rich in phenolic bioactive compounds is associated with a lower risk of neurodegenerative diseases and better cognitive performance in aged populations. Since oxidative stress is a common hallmark of age-related cognitive decline, incorporating exogenous antioxidants could have beneficial effects on brain aging. In this review, we firstly summarize and critically discuss the current preclinical evidence and the potential neuroprotective mechanisms. Existing studies indicate that olive oil phenolic compounds can modulate and counteract oxidative stress and neuroinflammation, two relevant pathways linked to the onset and progression of neurodegenerative processes. Secondly, we summarize the current clinical evidence. In contrast to preclinical studies, there is no direct evidence in humans of the bioactivity of olive oil phenolic compounds. Instead, we have summarized current findings regarding nutritional interventions supplemented with olive oil on cognition. A growing body of research indicates that high consumption of olive oil phenolic compounds is associated with better preservation of cognitive performance, conferring an additional benefit, independent of the dietary pattern. In conclusion, the consumption of olive oil rich in phenolic bioactive compounds has potential neuroprotective effects. Further research is needed to understand the underlying mechanisms and potential clinical applications.
Temperature Sensing in Space and Transparent Media: Advancements in Off-Axis Digital Holography and the Temperature Coefficient of Refractive Index
Authors: Khodadad Davood , Tayebi Behnam , Saremi Amin , Paul Satyam
Published: July 2023

An off-axis digital holographic interferometry technique integrated with a Mach–Zehnder interferometer based setup is demonstrated for measuring the temperature and temperature profile of a transparent medium. This technique offers several advantages: it does not require precise optomechanical adjustments or accurate definition of the frequency carrier mask, making it simple and cost-effective. Additionally, high-quality optics are not necessary. The methodology relies on measuring the phase difference between two digitally reconstructed complex wave fields and utilizing the temperature coefficient of the refractive index. In this way, we presented an equation of the temperature as a function of phase changes and the temperature coefficient of refractive index. This approach simplifies the calculation process and avoids the burden of complicated mathematical inversions, such as the inverse Abel transformation. It also eliminates the need for additional work with the Lorentz–Lorentz equation and Gladstone–Dale relation and can be extend for 3D measurements.
Dynamic Mechanical Behavior of Rock Specimens with Varying Joint Roughness and Inclination under Impact Load
Authors: Yuan Pu , Li Aobo , Chen Changning , Zhang Qinghe
Published: July 2023

The influence of joint roughness and inclination on the dynamic mechanical properties of rocks is a prominent research area. In order to investigate the effects of joint roughness and inclination on the dynamic mechanical properties of jointed rocks, impact tests were conducted using a spilt Hopkinson pressure bar (SHPB) apparatus for prefabricated serrated joint cement mortar specimens with varying joint roughness and inclination. The failure mode, stress wave propagation characteristics, peak stress, and stress wave energy transfer law under impact load were analyzed. The results indicate that both joint inclinations and joint roughness coefficients (JRC) have a strong influence on the failure mode, peak stress, and stress wave energy transfer of jointed specimens. The jointed specimens exhibit three distinct failure modes under impact load, namely splitting tensile failure, shear slip failure, and compound failure of splitting tensile and shear. The peak stress initially decreases then increases with the increase in joint inclination angle, namely a V-shape variation, while it gradually increases with JRC increasing from 0 to 20. Jointed specimens exhibit the lowest peak stress at an inclination angle of 45° and JRC of 0. The variation of transmitted energy coefficient is similar to the peak stress, while the variation of reflected energy coefficient is opposite to the peak stress. At joint inclination angles of 0° or 90°, the reflected energy coefficient increases with the increase of JRC from 0 to 20, while the transmitted energy decreases. However, when the joint inclination angle is in the range of 30° to 60°, the reflected energy coefficient gradually decreases with JRC increasing, while the transmitted energy coefficient gradually increases.