bluuebunny/crossref_metadata_2025 huggingface dataset:
Perilaku Petugas Linen di Rumah Sakit Umum Daerah (RSUD) Anuntaloko Kabupaten Parigi Moutong
Authors: Dja'afar Tjitrowati , Saharudin Saharudin , Bungawati Andi , Maryam Maryam , Syam Dedi Mahyudin
Published: October 2022

Pendahuluan: Rumah sakit bertujuan memberi pelayanan kesehatan, yang meliputi pelayanan medis, penunjang medis dan penunjang non medis. Pengelolaan linen yang buruk akan menyebabkan potensi penularan penyakit bagi pasien, staf dan pengguna linen. Linen kotor tidak terinfeksi adalah linen yang tidak terkontaminasi oleh darah, cairan tubuh dan feses yang berasal dari pasien dan lainnya. Tujuan: Tujuan penelitian ini untuk mengetahui gambaran perilaku petugas linen di rumah sakit Anuntaloko parigi Moutong.
Metode: Jenis penelitian deskriptif dengan pendekatan observasi. Populasi dalam penelitian ini adalah petugas linen di rumah sakit Anuntaloko Parigi yang berjumlah keseluruhan 13 orang dan sampel dalam penenelitian ini menggunakan metode sampling jenuh, yang artinya seluruh dari populasi diteliti yaitu 13 orang petugas linen di rumah sakit. Hasil: Hasil penelitian ini menunjukan bahwa pengatahuan petugas linen yang memiliki kriteria baik berjumlah 10 responden  (76,9%) , cukup berjumlah 3 responden  (23,1%) dan kurang berjumlah 0 (0%), sikap petugas linen di rumah sakit Anuntaluko adalah positif, tindakan petugas linen di rumah sakit Anuntaloko untuk tahapan pencucian linen mulai dari tahapan pengumpulan, pengeringan, penyetrikaan, penyimpanan, distribusi dan pengangkutan linen non infeksiusdan infeksius telah dilakukan sesuai dengan prosedur pencucian linen, sedangkan pada tahapan penimbangan dan pencucian linen non infeksiusdan infeksius belum semua dilakukan sesuai dengan prosedur pencucian linen. Kesimpulan: Petugas linen di rumah sakit umum daerah (RSUD) Anuntaloka Parigi Moutong  rata-rata untuk pengetahuan adalah baik, sikap menunjukkan positif, dan tindakan sudah sesuai dengan peraturan yang ditetapkan.
Structural regulation and dynamic behaviour of organelles during plant meiosis
Authors: Koç Aybars , De Storme Nico
Published: October 2022

Eukaryotes use various mechanisms to maintain cell division stability during sporogenesis, and in particular during meiosis to achieve production of haploid spores. In addition to establishing even chromosome segregation in meiosis I and II, it is crucial for meiotic cells to guarantee balanced partitioning of organelles to the daughter cells, to properly inherit cellular functions. In plants, cytological studies in model systems have yielded insights into the meiotic behaviour of different organelles, i.e., clearly revealing a distinct organization at different stages throughout meiosis indicating for an active regulatory mechanism determining their subcellular dynamics. However, how, and why plant meiocytes organize synchronicity of these elements and whether this is conserved across all plant genera is still not fully elucidated. It is generally accepted that the highly programmed intracellular behaviour of organelles during meiosis serves to guarantee balanced cytoplasmic inheritance. However, recent studies also indicate that it contributes to the regulation of key meiotic processes, like the organization of cell polarity and spindle orientation, thus exhibiting different functionalities than those characterized in mitotic cell division. In this review paper, we will outline the current knowledge on organelle dynamics in plant meiosis and discuss the putative strategies that the plant cell uses to mediate this programmed spatio-temporal organization in order to safeguard balanced separation of organelles. Particular attention is thereby given to putative molecular mechanisms that underlie this dynamic organelle organization taken into account existing variations in the meiotic cell division program across different plant types. Furthermore, we will elaborate on the structural role of organelles in plant meiosis and discuss on organelle-based cellular mechanisms that contribute to the organization and molecular coordination of key meiotic processes, including spindle positioning, chromosome segregation and cell division. Overall, this review summarizes all relevant insights on the dynamic behaviour and inheritance of organelles during plant meiosis, and discusses on their functional role in the structural and molecular regulation of meiotic cell division.
Graphitic carbon nitride (g-C3N4)-based photocatalytic materials for hydrogen evolution
Authors: Gao Rui-Han , Ge Qingmei , Jiang Nan , Cong Hang , Liu Mao , Zhang Yun-Qian
Published: October 2022

The semiconductors, such as TiO2, CdS, ZnO, BiVO4, graphene, produce good applications in photocatalytic water splitting for hydrogen production, and great progress have been made in the synthesis and modification of the materials. As a two-dimensional layered structure material, graphitic carbon nitride (g-C3N4), with the unique properties of high thermostability and chemical inertness, excellent semiconductive ability, affords good potential in photocatalytic hydrogen evolution. However, the related low efficiency of g-C3N4 with fast recombination rate of photogenerated charge carriers, limited visible-light absorption, and low surface area of prepared bulk g-C3N4, has called out the challenge issues to synthesize and modify novel g-C3N4-block photocatalyst. In this review, we have summarized several strategies to improve the photocatalytic performance of pristine g-C3N4 such as pH, morphology control, doping with metal or non-metal elements, metal deposition, constructing a heterojunction or homojunction, dye-sensitization, and so forth. The performances for photocatalytic hydrogen evolution and possible development of g-C3N4 materials are shared with the researchers interested in the relevant fields hereinto.
Predictors and long-term outcomes of heart block after transcatheter device closure of perimembranous ventricular septal defect
Authors: Jiang Diandong , Zhang Simiao , Zhang Yuxin , Lv Jianli , Yi Yingchun , Wang Jing , Wang Yan , Yang Xiaofei , Zhang Jianjun , Han Bo
Published: October 2022

Background

Heart block is the most common and concerning complication associated with transcatheter device closure of perimembranous ventricular septal defect (pmVSD) and its occurrence remains a great challenge for device closure.

Methods

Between June 2002 and June 2020, 1076 pediatric patients with pmVSD, who successfully underwent transcatheter device closure in our center, were enrolled in this cohort study, with a median follow-up of 64 months (range: 1 to 19 years).

Results

Of 1076 patients, 234 (21.8%) developed postprocedural heart block, with right bundle branch block being the most common (74.8%), followed by left bundle branch block (16.2%), and atrioventricular block (5.6%). Complete atrioventricular block occurred in 5 cases, including 3 cases with permanent pacemaker implantation, 1 case with recovery to normal sinus rhythm, and 1 case with sudden cardiac death. Most patients (97.9%) developed heart block within 1 week of procedure. Finally, 138 cases returned to normal cardiac conduction. Multivariate logistic regression revealed that thin-waist occluders (odds ratio [OR]: 1.759; 95% confidence interval [CI]: 1.023 to 3.022; P = 0.041), and oversized devices (OR: 1.809; 95% CI: 1.322 to 2.476; P < 0.001) were independently associated with occurrence of postprocedural heart block. Moreover, heart block was less likely to occur when the left disk of occluder was placed within the aneurysmal tissue (OR: 0.568; 95% CI: 0.348 to 0.928; P = 0.024).

Conclusion

The outcome of postprocedural heart block is favorable in most cases. Oversized devices and thin-waist occluders should be avoided. Placement of the left disk of the device should into the aneurysmal tissue is highly recommended.
Research progress on risk factors related to intracranial artery, carotid artery, and coronary artery stenosis
Authors: Liu Ruijun , Shao Jing
Published: October 2022

In recent decades, with the rapid development of economy, the acceleration of social aging and urbanization, and the prevalence of unhealthy lifestyles, the number of patients with cardiovascular and cerebrovascular diseases has shown an increasing trend year by year. It has also become one of the important causes of disability and death in all ages and groups. Atherosclerosis is the main pathological change of ischemic cardiovascular and cerebrovascular diseases, which mainly invades the large and medium arteries of the body circulation. In particular, cerebral artery and coronary artery lesions have the most significant impact on life. There is the same pathogenic mechanism between intracranial and extracranial arteries and coronary atherosclerosis, so there is a certain relationship between the degree of atherosclerosis. In this paper, the risk factors related to intracranial and extracranial arteries and coronary artery stenosis were reviewed. It provides a theoretical basis for early detection, early diagnosis and early treatment of intracranial and extracranial artery and coronary artery stenosis to reduce the occurrence and development of cardiovascular and cerebrovascular diseases.
Incidence, recurrence and management of electrical storm in Brugada syndrome
Authors: El-Battrawy Ibrahim , Roterberg Gretje , Kowitz Jacqueline , Aweimer Assem , Lang Siegfried , Mügge Andreas , Zhou Xiaobo , Akin Ibrahim
Published: October 2022

Background

Brugada syndrome (BrS) is associated with ventricular tachyarrhythmias. However, the presence of electrical strom (ES) and its management still debated.

Objectives

We present the outcome and management of 44 BrS patients suffering from ES.

Methods

A systematic literature review and pooled analysis Through database review including PubMed, Web of Science, Cochrane Libary and Cinahl studies were analyzed. Evidence from 7 reports of 808 BrS patients was identified.

Results

The mean age of patients suffering from ES was 34 ± 9.5 months (94.7% males, 65.8% spontaneous BrS type I). Using electrophysiological study ventricular tachycardia/ventricular fibrillation were inducible in 12/23 (52.2%). Recurrence of ES was documented in 6.1%. Death from ES was 8.2% after a follow-up of 83.5 ± 53.4. In up to 27 ES resolved without treatment. External shock was required in 35.6%, internal ICD shock in 13.3%, Overdrive pacing, left cardiac sympathetic block and atropin in 2.2%. Short-term antiarrhythmic management was as the following: Isopreterenol or Isopreterenol in combination with quinidine 35.5%, orciprenaline in 2.2%, quinidine 2.2%, disopyramide 2.2% or denopamide 2.2%. However, lidocaine, magensium sulfate, mexiletine and propanolol failed to control ES.

Conclusion

Although ES is rare in BrS, this entity challenges physicians. Despite its high mortality rate, spontaneous termination is possible. Short-term management using Isoproterenol and/or quinidine might be safe. Prospective studies on management of ES are warranted.
Five principles for high-quality mathematics teaching: Combining normative, epistemological, empirical, and pragmatic perspectives for specifying the content of professional development
Authors: Prediger Susanne , Götze Daniela , Holzäpfel Lars , Rösken-Winter Bettina , Selter Christoph
Published: October 2022

The academic search for principles of high-quality subject-matter teaching has been informed by different perspectives, in particular normative, epistemological, empirical, and pragmatic perspectives. While these perspectives have sometimes been treated as competing, we emphasize the need of their integration to identify sets of principles that can inform professional development programs for quality development. This paper starts from characterizing the four perspectives, and then shows how they are iteratively intertwined in providing a research base for specifying high-quality principles for teaching, in our case exemplified for the school subject mathematics. For this goal, we present the set of five principles that we have deemed as core principles for a new nationwide, ten-year professional development program in Germany: Conceptual Focus, Cognitive Demand, Student Focus and Adaptivity, Longitudinal Coherence, and Enhanced Communication. We will discuss these five principles against their backgrounds stemming from combing normative, epistemological, empirical, and pragmatic perspectives. This set of principles serves as an exemplary case to substantiate our general argumentation that contemporary educational research and professional development activities should not choose between perspectives but strive for combining them.
Nonlinear relationship between aspartate aminotransferase to alanine aminotransferase ratio and the risk of prediabetes: A retrospective study based on chinese adults
Authors: Cao Changchun , Zhang Xiaohua , Yuan Junhu , Zan Yibing , Zhang Xin , Xue Chao , Wang Yulong , Zheng Xiaodan
Published: October 2022

Objective

Recent evidence has revealed that the aspartate aminotransferase to alanine aminotransferase ratio (AST/ALT ratio) may be closely associated with metabolic syndrome and insulin resistance. However, it is unclear whether the AST/ALT ratio correlates with prediabetes risk. The aim of our study was to examine the association between AST/ALT ratios and the risk of prediabetes among a large cohort of Chinese subjects.

Methods

This retrospective cohort study recruited 75204 Chinese adults with normoglycemia at baseline who underwent physical examinations at the Rich Healthcare Group from 2010 to 2016. The AST/ALT ratio at baseline was the target independent variable, and the risk of developing prediabetes during follow-up was the dependent variable. Cox proportional-hazards regression was used to evaluate the independent association between the AST/ALT ratio and prediabetes. This study identified nonlinear relationships by applying a generalized additive model (GAM) and smooth curve fitting. In order to assess the robustness of this study, we performed a series of sensitivity analyses. Moreover, we performed a subgroup analysis to evaluate the consistency of the association in different subgroups. Data from this study have been updated on the DATADRYAD website.

Results

The AST/ALT ratio was negatively and independently related to the prediabetes risk among Chinese adults (HR: 0.76, 95% CI: 0.75-0.84, P<0.0001) after adjusting demographic and biochemical covariates. Furthermore, a nonlinear relationship between the AST/ALT ratio and the risk of developing prediabetes was found at an inflection point of 1.50 for the AST/ALT ratio. When the AST/ALT ratio was to the left of the inflection point (AST/ALT ratio ≤ 1.50), the AST/ALT ratio was negatively related to the prediabetes risk (HR:0.70, 95%CI: 0.65-0.76, P<0.0001). In contrast, the relationship tended to be saturated when the AST/ALT ratio was more than 1.50 (HR: 1.01, 95%CI: 0.89-1.15, P=0.8976). Our findings remained robust across a range of sensitivity analyses. Subgroup analysis revealed that other variables did not alter the relationship between the AST/ALT ratio and prediabetes risk.

Conclusion

This study revealed that AST/ALT ratio was negatively and independently associated with prediabetes risk among Chinese participants. The relationship between the AST/ALT ratio and prediabetes risk was nonlinear, and AST/ALT ratio ≤ 1.50 was strongly inversely correlated with prediabetes risk.
Agricultural land use and pond management influence spatial-temporal variation of CH4 and N2O emission fluxes in ponds in a subtropical agricultural headstream watershed
Authors: Fan Manman , Zhang Wenzhao , Wu Jingtao , Zhou Jiaogen
Published: October 2022

Small water bodies are hotspots of biogeochemical cycles with large spatial and temporal heterogeneity of their greenhouse gas emission fluxes. To reveal the spatial and temporal variabilities of methane (CH4) and nitrogen dioxide (N2O) emission fluxes in small water bodies in subtropical agricultural headwater watersheds, monthly measurements of CH4 and N2O fluxes were performed in 53 ponds from May 2018 to July 2020. Results showed that the fluxes of CH4 and N2O exhibited distinct spatial and temporal variations, and generally showed a trend of high emission rate in summer and low emission rate in winter. Agricultural land use and pond management had important impacts on CH4 and N2O emission fluxes in ponds. The CH4 and N2O emission fluxes were significantly higher in the ponds with the landscape of farmland, tea plantation and residential area than those of forest, which might be relevant to the eutrophication of pond water bodies. The mean N2O emission flux in intensive fishing ponds was much higher than that in grass-fed fishing ponds and no fishing ponds. Moreover, the sustained-flux global warming potential (SGWP) in the eutrophic pond water bodies of farmland, tea plantation and residential area are significantly higher than that in oligotrophic water bodies of forest ponds. Our CH4 and N2O emission results suggest that the reduction potential of 4.98 kg C ha−1 y−1 for CH4 and 0.42 kg N ha−1 y−1 for N2O will occur in future if ecological management measures are implemented to improve the pond water quality from eutrophication to oligotrophic conditions.
Financing energy transition in COVID-19 for energy efficiency: Does mitigation of energy price risk that essential?
Authors: Shao Ming , Wang Lulu
Published: October 2022

The aim of the study is to test the nexus between energy prices risk and energy efficiency for energy transition to recommend the implication for the period of COVID-19 crises. This study uses short-term daily data of G-20 countries for COVID-19 crises was taken to better estimate the study findings. To estimate the results Data Envelopment Analysis estimation method was applied for empirical inference. The findings revealed that the COVID-19 crises have the biggest negative influence with 47.1% on oil prices which raised energy prices risk at large. The aforementioned results hold even after substituting the magnitude of extreme list of variables, re-estimating the equations with energy efficiency. Such risk has further halted energy efficiency with 33.69% in G-20 countries during COVID-19 which is one of the largest dent of the mankind history. Our results also inferred that the relationship of energy prices risk with energy efficiency remained significant but negative with energy transition. Extending to it, the post-COVID-19, the danger of energy stock price crashes is expected to diminish dramatically. The study suggest that the corporations must participate in greater corporate social responsibility activities have lower post-COVID-19 energy price collapse risk. Finally, we demonstrate that post-COVID-19, the impact of COVID-19 on energy price collapse risk.
A feasibility study of noninvasive prenatal diagnosis in facioscapulohumeral muscular dystrophy type 1 in a Chinese family
Authors: Qin Yayun , Xu Hui , Yang Jingmin , Wu Yiming , Li Hui , Wang Bo , Liu Lijun , Ren Ding , Xu Runhong , Li Manman , Zhang Chengcheng , Song Jieping
Published: October 2022

Objective: To demonstrate the feasibility of haplotype-based noninvasive prenatal diagnosis of Facioscapulohumeral Muscular Dystrophy type 1 (FSHD1).

Methods: Bionano optical mapping was used to identify the D4Z4 structural variation of the genomic DNA sample from the proband affected with FSHD1. In addition, based on the technique of next generation sequencing, the pathogenic haplotype was determined by using trio strategy through genotyping his parents, and also fetal inheritance of paternal haplotypes was then deduced using the Hidden Markov Model.

Results: Bionano optical mapping analysis revealed that the proband has only three D4Z4 repeats left in the 4q35 chromosomal region and a disease-permitting 4qA haplotype. The other normal allele of the proband contains 29 D4Z4 repeats and also a 4qA haplotype. The noninvasive cell-free fetal DNA (cffDNA)-based haplotype analysis suggested that the fetus inherited the pathogenic allele from his father and thus was predicted to be affected by FSHD1. In addition, Bionano optical mapping also demonstrated the presence of the pathogenic allele in the fetus by interrogating the genomic DNA from the amniotic fluid cells.

Conclusion: Our study showed the cffDNA-based haplotyping was feasible for the noninvasive prenatal diagnosis of FSHD1, which is able to provide earlier testing results with a lower risk of miscarriage and infection than invasive techniques.
Comparative mitogenomes provide new insights into phylogeny and taxonomy of the subfamily Xenocyprinae (Cypriniformes: Cyprinidae)
Authors: Zhang Zhi , Li Jiming , Zhang Xiaodong , Lin Bingming , Chen Jianming
Published: October 2022

Xenocyprinae is a cyprinid subfamily that not only has a discrete geographic distribution but also has a long history dating to the Early Miocene. However, it is controversial whether systematic classification and some species validity of Xenocyprinae exist, as well as its phylogenetic relationships and evolutionary history. In the present study, we first reviewed the description and taxonomic history of Xenocyprinae, and then the complete mitochondrial genome of Distoechodon compressus, an endemic and locally distributed species belonging to Xenocyprinae, was sequenced and annotated. Finally, all the mitogenomes of Xenocyprinae were compared to reconstruct the phylogenetic relationship and estimate the divergence time. The results showed that the mitogenomes are similar in organization and structure with 16618–16630 bp length from 12 mitogenomes of eight species. Phylogenetic analysis confirmed the monology of Xenocyprinae and illustrated three clades within the Xenocyprinae to consist of ambiguous generic classification. Plagiognathops is a valid genus located at the base of the phylogenetic tree. The genus Xenocypris was originally monophyletic, but X. fangi was excluded. Divergence time estimation revealed that the earliest divergence within Xenocyprinae occurred approximately 12.1 Mya when Plagiognathops separated from the primitive Xenocypris. The main two clades (Xenocypris and (Distoechodon + Pseudobrama + X. fangi)) diverged 10.0 Mya. The major divergence of Xenocyprinae species possibly occurred in the Middle to Late Miocene and Late Pliocene, suggesting that speciation and diversifications could be attributed to the Asian monsoon climate. This study clarifies some controversial issues of systematics and provides essential information on the taxonomy and phylogeny of the subfamily Xenocyprinae.
Clinical manifestations and genetic analysis of a newborn with Arboleda−Tham syndrome
Authors: Zeng Feng , Yang Yue , Xu Zhaohui , Wang Ziwen , Ke Huan , Zhang Jianhong , Dong Tongtong , Yang Wenming , Wang Jiuxiang
Published: October 2022

Arboleda−Tham syndrome (ARTHS) is a rare disorder first characterized in 2015 and is caused by mutations in lysine (K) acetyltransferase 6A (KAT6A, a.k.a. MOZ, MYST3). Its clinical symptoms have rarely been reported in newborns from birth up to the first few months after birth. In this study, a newborn was diagnosed with ARTHS based on the clinical symptoms and a mutation c.3937G>A (p.Asp1313Asn) in KAT6A. The clinical manifestations, diagnosis, and treatment of the newborn with ARTHS were recorded during follow-up observations. The main symptoms of the proband at birth were asphyxia, involuntary breathing, low muscle tone, early feeding, movement difficulties, weak crying, weakened muscle tone of the limbs, and embrace reflex, and facial features were not obvious at birth. There was obvious developmental delay, as well as hypotonic and oro-intestinal problems in the first few months after birth. Mouse growth factor was used to nourish the brain nerves, and touching, kneading the back, passive movements of the limbs, and audio−visual stimulation were used for rehabilitation. We hope that this study expands the phenotypic spectrum of this syndrome to newborns and the library of KAT6A mutations that lead to ARTHS. Consequently, the data can be used as a basis for genetic counseling and in clinical and prenatal diagnosis for ARTHS prevention.
Immunosuppressive adenosine-targeted biomaterials for emerging cancer immunotherapy
Authors: Wei Qi , Zhang Lening , Zhao Nan , Cheng Zhihua , Xin Hua , Ding Jianxun
Published: October 2022

Immunotherapy has paved the way for the future of cancer therapy, but there are still significant challenges to be overcome, such as the occurrence of immune escape or suppression. Adenosine is essential in modulating the immune responses of immune cells and maintaining immune tolerance. Emerging adenosine pathway inhibitors are considered a breakthrough in cancer immunotherapy, with emphasis first being placed on the top-down blockade of adenosine signaling axis, followed by combination therapy. However, these therapeutic strategies rely on adenosine inhibitors, mainly small molecules or antibody proteins, which are limited by a single route of administration and off-target toxicity. Therefore, synergistic nanomedicine with accurate delivery targeting deeper tumors is focused on in preclinical studies. This review discusses how adenosine reshapes immunosuppressive microenvironments through its effects on immune cells, including lymphocytes and myeloid cells. Additionally, it will be the first discussion of a comprehensive strategy of biomaterials in modulating the adenosine signaling pathway, including inhibition of adenosine production, inhibition of adenosine binding to immune cells, and depletion of adenosine in the microenvironments. Furthermore, biomaterials integrating multiple therapeutic modalities with adenosine blocking are also discussed as a promising strategy for promoting cancer immunotherapy.
Cross-reactive neutralizing human monoclonal antibodies mapping to variable antigenic sites on the norovirus major capsid protein
Authors: Ford-Siltz Lauren A. , Tohma Kentaro , Alvarado Gabriela S. , Kendra Joseph A. , Pilewski Kelsey A. , Crowe James E. , Parra Gabriel I.
Published: October 2022

Human noroviruses are the major viral cause of acute gastroenteritis around the world. Although norovirus symptoms are in most cases mild and self-limited, severe and prolonged symptoms can occur in the elderly and in immunocompromised individuals. Thus, there is a great need for the development of specific therapeutics that can help mitigate infection. In this study, we sought to characterize a panel of human monoclonal antibodies (mAbs; NORO-123, -115, -273A, -263, -315B, and -250B) that showed carbohydrate blocking activity against the current pandemic variant, GII.4 Sydney 2012. All antibodies tested showed potent neutralization against GII.4 Sydney virus in human intestinal enteroid culture. While all mAbs recognized only GII.4 viruses, they exhibited differential binding patterns against a panel of virus-like particles (VLPs) representing major and minor GII.4 variants spanning twenty-five years. Using mutant VLPs, we mapped five of the mAbs to variable antigenic sites A (NORO-123, -263, -315B, and -250B) or C (NORO-115) on the major capsid protein. Those mapping to the antigenic site A showed blocking activity against multiple variants dating back to 1987, with one mAb (NORO-123) showing reactivity to all variants tested. NORO-115, which maps to antigenic site C, showed reactivity against multiple variants due to the low susceptibility for mutations presented by naturally-occurring variants at the proposed binding site. Notably, we show that cross-blocking and neutralizing antibodies can be elicited against variable antigenic sites. These data provide new insights into norovirus immunity and suggest potential for the development of cross-protective vaccines and therapeutics.
Seasonal variability of the North Equatorial Current–Kuroshio Current–Mindanao Current based on observations
Authors: Wang Fujun , Zhang Linlin , Feng Junqiao , Hu Dunxin
Published: October 2022

Utilizing the direct mooring observation along 130°E (10.5°N, 13°N, 15.5°N, and 18°N), 18°N (122.7°E, 123°E, and 123.3°E), and 8°N (127°E) and satellite data, the seasonal variabilities of the North Equatorial Current (NEC), Kuroshio Current (KC), and Mindanao Current (MC) were investigated. The southern part of the NEC along 130°E, the KC along 18°N, and the MC along 8°N featured similar seasonal cycles: that is, the currents were strong in spring and weak in autumn, while the KC along 18°N featured another peak in winter. Moreover, the seasonal phase of the NEC along 130°E was latitude-dependent; it advanced slightly from 10°N to 14°N and delayed poleward. The seasonal variabilities of the three currents were mainly controlled by local winds and Rossby waves via a geostrophic relationship, as the mooring observation was consistent with satellite data. The calculation shows that the local wind was dominant in the above mentioned in-phase areas (i.e., the southern part of the NEC, KC, and MC), while Rossby waves played an important role in the northern part of the NEC. The results indicate that the NEC–KC–MC system had the same zonal dynamics but different meridional dynamics across seasons.
Soil organic carbon stabilization and associated mineral protection in typical coastal wetlands under different hydrologic conditions
Authors: Zhao Haixiao , Jia Jia , Zhao Qingqing , Wang Jianing , Gao Yongchao , Huang Yujie , Chen Guanhong
Published: October 2022

The soil carbon sequestration largely depends on soil organic carbon (SOC) stability. However, the mechanism of SOC stabilization in coastal wetlands under different hydrologic conditions remains to be clarified. In this study, the effect of mineral protection on SOC stabilization was investigated in freshwater-flooded Phragmites australis wetlands (FPW), tidal P. australis wetlands (TPW), non-flooded P. australis wetlands (NPW) and tidal Suaeda salsa wetlands (TSW). Two stabilized SOC fractions (Na2S2O8 resistant SOC and H2O2 resistant SOC) and Al/Fe/Si oxides extracted by acid oxalate, dithionite–citrate and pyrophosphate, respectively, were determined in soil samples collected to a depth of 25 cm. The contents of soil Na2S2O8 resistant SOC and H2O2 resistant SOC were not significantly different among the four wetlands (p > 0.05), with the highest mean values occurring in FPW (1.44 ± 0.43 g kg-1) and TPW (1.79 ± 0.40 g kg-1), respectively. The contents of Al/Fe/Si oxides (except for Sip) and the values of mineral phase indicators were significantly lower in NPW than in TPW or FPW (p < 0.05). Linear regressions among Al/Fe/Si oxides, mineral phase indicators, and two stabilized SOC fractions showed that Na2S2O8 resistant SOC and H2O2 resistant SOC were positively and significantly affected by mineral contents (p < 0.05). Additionally, edaphic factors such as soil organic matter, total nitrogen, soil water content, clay and silt contents were identified as important factors influencing the two stabilized SOC fractions.
Spatiotemporal resilience assessment and comparison in China’s bay area
Authors: Han Yu , Zhang Yaowen , Zhang Han , Feng Cuicui , Hua Tianran , Yang Yiqun , Yun Peng , Zeng Jian , Peng Li , Zeng Jiangning , Ye Guanqiong
Published: October 2022

The bay area is a crucial land–sea junction zone containing essential urban clusters while receiving extremely complex internal and external disturbances that challenge more on its resilience management. However, a sound management tool based on the bay area’s resilience is widely lacking due to the difficulty of unifying resilience indicators and quantifying resilience relationships between regions. This paper tries to establish a comprehensive resilience index for coastal bays from four major resilience-related dimensions, namely, physical structure, social development, ecological environment, and hazards, and applies it into the three major developed bay areas in China. A coupling coordination degree model was used to further reveal the resilience development and its internal coordination by temporal and spatial differences. The results show that the index could clearly reveal the resilience changes from the year 2000 to 2020 of the three bays with the common key drivers of socioeconomic development. It also explains the resilience changes among three bay areas through analyzing synergistic and conflict relationships between the four sub-resilience systems.
Artificial intelligence in ultrasound-guided regional anesthesia: A scoping review
Authors: Viderman Dmitriy , Dossov Mukhit , Seitenov Serik , Lee Min-Ho
Published: October 2022

Background

Regional anesthesia is increasingly used in acute postoperative pain management. Ultrasound has been used to facilitate the performance of the regional block, increase the percentage of successfully performed procedures and reduce the complication rate. Artificial intelligence (AI) has been studied in many medical disciplines with achieving high success, especially in radiology. The purpose of this review was to review the evidence on the application of artificial intelligence for optimization and interpretation of the sonographic image, and visualization of needle advancement and injection of local anesthetic.

Methods

To conduct this scoping review, we followed the PRISMA-S guidelines. We included studies if they met the following criteria: (1) Application of Artificial intelligence-assisted in ultrasound-guided regional anesthesia; (2) Any human subject (of any age), object (manikin), or animal; (3) Study design: prospective, retrospective, RCTs; (4) Any method of regional anesthesia (epidural, spinal anesthesia, peripheral nerves); (5) Any anatomical localization of regional anesthesia (any nerve or plexus) (6) Any methods of artificial intelligence; (7) Settings: Any healthcare settings (Medical centers, hospitals, clinics, laboratories.

Results

The systematic searches identified 78 citations. After the removal of the duplicates, 19 full-text articles were assessed; and 15 studies were eligible for inclusion in the review.

Conclusions

AI solutions might be useful in anatomical landmark identification, reducing or even avoiding possible complications. AI-guided solutions can improve the optimization and interpretation of the sonographic image, visualization of needle advancement, and injection of local anesthetic. AI-guided solutions might improve the training process in UGRA. Although significant progress has been made in the application of AI-guided UGRA, randomized control trials are still missing.
The regulation of antiviral activity of interferon epsilon
Authors: Zhao Fu-Rong , Wang Wei , Zheng Qin , Zhang Yan-Ge , Chen Jianming
Published: October 2022

Interferon epsilon (IFN-ε) is a type I IFN. Some biological properties has been identified in many species, such as antiproliferative, anti-tumor, and antiviral effects, of IFN-ε, which are much weaker than those of IFN-α, have also been revealed. It has been shown to play a role in mucosal immunity and bacterial infection and in the prevention of certain sexually transmitted diseases, such as human immunodeficiency virus (HIV). This paper reviews the known activity of IFN-ε, particularly in some viruses. In general, this review provides a better understanding of effective IFN-ε treatment in the future.
Hemorrhagic fever viruses: Pathogenesis, therapeutics, and emerging and re-emerging potential
Authors: Flórez-Álvarez Lizdany , de Souza Edmarcia Elisa , Botosso Viviane Fongaro , de Oliveira Danielle Bruna Leal , Ho Paulo Lee , Taborda Carlos Pelleschi , Palmisano Giuseppe , Capurro Margareth Lara , Pinho João Renato Rebello , Ferreira Helena Lage , Minoprio Paola , Arruda Eurico , de Souza Ferreira Luís Carlos , Wrenger Carsten , Durigon Edison Luiz
Published: October 2022

Hemorrhagic fever viruses (HFVs) pose a threat to global public health owing to the emergence and re-emergence of highly fatal diseases. Viral hemorrhagic fevers (VHFs) caused by these viruses are mostly characterized by an acute febrile syndrome with coagulation abnormalities and generalized hemorrhage that may lead to life-threatening organ dysfunction. Currently, the events underlying the viral pathogenicity associated with multiple organ dysfunction syndrome still underexplored. In this minireview, we address the current knowledge of the mechanisms underlying VHFs pathogenesis and discuss the available development of preventive and therapeutic options to treat these infections. Furthermore, we discuss the potential of HFVs to cause worldwide emergencies along with factors that favor their spread beyond their original niches.
Photometric method for dual targeting of surface and surface-associated proteins on extracellular vesicles in the multiparametric test
Authors: Clegg Lee-Ann Marie , Sloth Jenni Kathrine , Bæk Rikke , Jørgensen Malene Møller
Published: October 2022

Extracellular vesicles (EVs) have become a topic of interest within the field of diagnostic biomarkers; however, recent developments in the study of EVs have increased the need for simpler but still comprehensive methods for characterization. Here, we describe how to simultaneously measure several surface or surface-associated proteins on EVs using a multiparametric microarray-based analysis termed Extracellular Vesicle Array (EV Array), which is developed to catch and phenotypically characterize small EVs. Previously, this analysis has been limited to measuring only one fluorescent signal per analysis. The analysis relies on antibodies printed onto a solid surface, for catching the EVs carrying the specific surface or surface-associated proteins, and on the subsequent fluorescent detection. For the optimization of detection, two antibodies with attached Cy3 or Cy5 were added to various combinations of the EV surface or surface-associated proteins: CD9, CD63, CD81, flotillin-1, and HSP90. In this study, the EV surface or surface-associated proteins were analyzed in human plasma from six healthy subjects. Changes observed in signal intensities from Cy3 and Cy5 related specifically to these combinations and allowed for a comparison of the two different fluorescent signals. When comparing the results, it was observed that it is possible to measure the EV surface or surface-associated proteins at both 532 nm (Cy3) and 635 nm (Cy5) simultaneously without a significant change in signals from the detection molecules. This allows us to measure multiple EV marker proteins in a single analysis, thereby more quickly finding complex biomarker patterns in a sample.
Risk profile, clinical presentation, and determinants of stroke subtypes among patients with stroke admitted to public referral hospitals, Northwest Ethiopia in 2021: A cross-sectional study
Authors: Ayehu Gashaw Walle , Yitbarek Getachew Yideg , Zewdie Edgiet Abebe , Amsalu Bedemariam Tadesse , Abie Yonatan , Atlaw Daniel , Agegnehu Assefa , Admasu Fitalew Tadele , Azanaw Melkalem Mamuye , Amare Abraham Tsedalu , Emiru Zemen Asmare
Published: October 2022

Background

Stroke is the second leading cause of death worldwide, with a significant increase in stroke burden over the last two and half decades, especially in developing countries. African countries are undergoing an epidemiological transition from being dominated by infectious diseases to being double-burdened by non-communicable diseases, with existing infectious diseases driven by sociodemographic and lifestyle changes and a weak healthcare system. Data on the risk profile, clinical presentation, and predictors of stroke subtypes are still limited. Therefore, the main aim of this study was to assess the risk profile, clinical presentation, and predictors of stroke in public referral hospitals of Northwest Ethiopia.

Methods

For this study, 554 patients with stroke admitted to three public referral hospitals were prospectively followed up. Data were collected using a pre-tested interviewer-administered questionnaire. STATA version 16 was used for data analyses. Candidate variables significant in bivariate analysis were selected for multivariate binary logistic regression, and statistical significance was set at a p < 0.05.

Results

Of the 554 patients with stroke, 60.3% had an ischemic stroke. The mean age of the participants was 61 ± 12.85 years, and more than half (53.25%) of them were women. The most common risk factor identified was hypertension (29.7%), followed by congestive heart failure. The most common clinical presentation was hemiparesis, which was reported by 57.7% of the patients, followed by loss of consciousness (20.7%) and aphasia (9%). Through multivariable logistic regression, age (AOR = 1.03, 95% CI:1.01–1.05), sedentary physical activity level (AOR = 6.78, 95% CI:1.97–23.32), absence of a family history of chronic illness (AOR = 3.79, 95% CI:2.21–6.48), hypertension (AOR=0.51, 95% CI:0.31–0.85), and past stroke (AOR = 3.54, 95% CI:0.93–13.49) were found to be independent determinants of the stroke subtype.

Conclusion

Age, the level of sedentary physical activity, absence of a family history of chronic illness, hypertension, and past stroke were independent determinants of stroke subtype.
Use of a bio-electronic device comprising of targeted dual neuromodulation of the hepatic and celiac vagal branches demonstrated enhanced glycemic control in a type 2 diabetic rat model as well as in an Alloxan treated swine model
Authors: Waataja Jonathan J. , Nihalani Raj K. , Honda Chris N. , Billington Charles J.
Published: October 2022

Background

There is an unmet need for new type 2 diabetes treatments providing improved efficacy, durability and customized to improve patient’s compliance. Bio-electronic neuromodulation of Vagus nerve branches innervating organs that regulate plasma glucose, may be a method for treating type 2 diabetes. The pancreas has been shown to release insulin during Vagus stimulation. The hepatic vagal branch, innervating the liver, has been shown to decrease glucose release and decrease insulin resistance following ligation. However, standalone stimulation of the Vagus nerve has shown mixed results and Vagus nerve ligation has undesirable effects. Little is known; however, of the effect on plasma glucose with combined neuromodulation consisting of stimulation of the celiac branch innervating the pancreas with simultaneous high frequency alternating current (HFAC) blockade of the hepatic branch. This study tested the effects of this approach on increasing glycemic control in rat a model of type 2 diabetes and Alloxan treated swine.

Materials and methods

Zucker obese (fatty) male rats (ZDF fa/fa) were used as a model of type 2 diabetes as well as glucose intolerant Alloxan treated swine. In ZDF rat experiments glycemic control was accessed with an intravenous glucose tolerance test during HFAC-induced hepatic branch block with concurrent celiac stimulation (HFAC + stimulation). In swine experiments glycemic control was accessed by an oral glucose tolerance test during HFAC + stimulation. Insulin measurements were taken prior to and following swine experiments giving insight into beta cell exhaustion. Histopathology was conducted to determine safety of HFAC + stimulation on Vagal branches.

Results

Zucker rats demonstrated a significant improvement to an intravenous glucose tolerance test during HFAC + stimulation compared to sham. There was no significant difference from sham compared to hepatic vagotomy or celiac stimulation. In Alloxan treated swine, when subjected to HFAC + stimulation, there was a significant improvement in glycemic control as measured by an improvement on oral glucose tolerance tests and a decrease in fasting plasma glucose. Insulin responses were similar prior to and following HFAC + stimulation experiments. Histopathology demonstrated healthy swine Vagus nerves.

Conclusion

Electrical blockade of the hepatic Vagus branch with simultaneous stimulation of the celiac Vagus branch may be a novel, adjustable and localized approach for a treatment of type 2 diabetes.
The hard problem of consciousness—A perspective from holistic philosophy
Authors: Chen Jicheng , Chen Linlin
Published: October 2022

Based on a material view and reductionism, science has achieved great success. These cognitive paradigms treat the external as an objective existence and ignore internal consciousness. However, this cognitive paradigm, which we take for granted, has also led to some dilemmas related to consciousness in biology and physics. Together, these phenomena reveal the interaction and inseparable side of matter and consciousness (or body and mind) rather than the absolute opposition. However, a material view that describes matter and consciousness in opposition cannot explain the underlying principle, which causes a gap in interpretation. For example, consciousness is believed to be the key to influencing wave function collapse (reality), but there is a lack of a scientific model to study how this happens. In this study, we reveal that the theory of scientific cognition exhibits a paradigm shift in terms of perception. This tendency implies that reconciling the relationship between matter and consciousness requires an abstract theoretical model that is not based on physical forms. We propose that the holistic cognitive paradigm offers a potential solution to reconcile the dilemmas and can be scientifically proven. In contrast to the material view, the holistic cognitive paradigm is based on the objective contradictory nature of perception rather than the external physical characteristics. This cognitive paradigm relies on perception and experience (not observation) and summarizes all existence into two abstract contradictory perceptual states (Yin-Yang). Matter and consciousness can be seen as two different states of perception, unified in perception rather than in opposition. This abstract perspective offers a distinction from the material view, which is also the key to falsification, and the occurrence of an event is inseparable from the irrational state of the observer’s conscious perception. Alternatively, from the material view, the event is random and has nothing to do with perception. We hope that this study can provide some new enlightenment for the scientific coordination of the opposing relationship between matter and consciousness.
Neuroinflammation in the medial prefrontal cortex exerts a crucial role in bone cancer pain
Authors: Li Xin , Wang Wei , Zhang Xiaoxuan , Gong Zhihao , Tian Mi , Zhang Yuxin , You Xingji , Wu Jingxiang
Published: October 2022

Bone cancer pain (BCP) is one of the most common types of pain in cancer patients which compromises the patient’s functional status, quality of life, and survival. Central hyperalgesia has increasingly been identified as a crucial factor of BCP, especially in the medial prefrontal cortex (mPFC) which is the main cortical area involved in the process of pain and consequent negative emotion. To explore the genetic changes in the mPFC during BCP occurrence and find possible targets for prediction, we performed transcriptome sequencing of mPFC in the BCP rat model and found a total of 147 differentially expressed mRNAs (DEmRNAs). A protein-protein interaction (PPI) network revealed that the DEmRNAs mainly participate in the inflammatory response. Meanwhile, microglia and astrocytes were activated in the mPFC of BCP rats, further confirming the presence of neuroinflammation. In addition, Gene Ontology (GO) analysis showed that DEmRNAs in the mPFC are mainly involved in antigen processing, presentation of peptide antigen, and immune response, occurring in the MHC protein complex. Besides, the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that DEmRNAs are mainly enriched in the pathways of phagosome, staphylococcus aureus infection, and antigen processing, in which MHCII participate. Furthermore, immunostaining showed that MHCII is mainly located in the microglia. Microglia are believed to be involved in antigen processing, a key cause of BCP. In vivo, minocycline (MC) treatment inhibits the activation of microglia and reduces the expression of MHCII and proinflammatory cytokines, thereby alleviating BCP and pain-related anxiety. Taken together, our study identified differentially expressed genes in the BCP process and demonstrated that the activation of microglia participates in the inflammatory response and antigen process, which may contribute to BCP.