bluuebunny/crossref_metadata_2025 huggingface dataset:
Solving the Fredholm Integral Equation by Common Fixed Point Results in Bicomplex Valued Metric Spaces
Authors: Abdou Afrah Ahmad Noman
Published: July 2023

The purpose of this research work is to explore the solution of the Fredholm integral equation by common fixed point results in bicomplex valued metric spaces. In this way, we develop some common fixed point theorems for generalized contractions containing point-dependent control functions in the context of bicomplex valued metric spaces. An illustrative and practical example is also given to show the novelty of the most important result.
Transient Dynamics in Counter-Rotating Stratified Taylor–Couette Flow
Authors: Godwin Larry , Trevelyan Philip , Akinaga Takeshi , Generalis Sotos
Published: July 2023

This study focuses on the investigation of stratified Taylor–Couette flow (STCF) using non-modal analysis, which has received relatively limited attention compared to other shear flows. The dynamics of perturbations under different temperature conditions are explored, and their patterns of amplification are analyzed. The study highlights the correlation between flow configurations, emphasizing the similarity in transient dynamics despite different speed ratios. The subcritical effects of thermal stratification on disturbance dynamics are examined, considering the interplay between viscous and buoyancy effects counteracted by strong centrifugal forces. It is found that increasing the wall temperature beyond a critical value leads to buoyancy forces dominating, resulting in a linear increase in the amplification factor. The research reveals significant deviations from previous results, indicating the significant role of temperature stratification.
Breast Cancer Diagnosis Using a Novel Parallel Support Vector Machine with Harris Hawks Optimization
Authors: Almotairi Sultan , Badr Elsayed , Abdul Salam Mustafa , Ahmed Hagar
Published: July 2023

Three contributions are proposed. Firstly, a novel hybrid classifier (HHO-SVM) is introduced, which is a combination between the Harris hawks optimization (HHO) and a support vector machine (SVM) is introduced. Second, the performance of the HHO-SVM is enhanced using the conventional normalization method. The final contribution is to improve the efficiency of the HHO-SVM by adopting a parallel approach that employs the data distribution. The proposed models are evaluated using the Wisconsin Diagnosis Breast Cancer (WDBC) dataset. The results show that the HHO-SVM achieves a 98.24% accuracy rate with the normalization scaling technique, outperforming other related works. On the other hand, the HHO-SVM achieves a 99.47% accuracy rate with the equilibration scaling technique, which is better than other previous works. Finally, to compare the three effective scaling strategies on four CPU cores, the parallel version of the proposed model provides an acceleration of 3.97.
Dissipative Discrete PID Load Frequency Control for Restructured Wind Power Systems via Non-Fragile Design Approach
Authors: Zhou Hanmei , Zhong Qishui , Hu Shaoyu , Yang Jin , Shi Kaibo , Zhong Shouming
Published: July 2023

This article proposes a discrete proportional-integral-derivative (PID) load frequency control (LFC) scheme to investigate the dissipative analysis issue of restructured wind power systems via a non-fragile design approach. Firstly, by taking the different power-sharing rates of governors into full consideration, a unified model is constructed for interconnected power systems containing multiple governors. Secondly, unlike existing LFC schemes, a non-fragile discrete PID control scheme is designed, which has the performance of tolerating control gain fluctuation and relieving the huge computational burden. Further, by constructing a discrete-type Lyapunov–Krasovskii functional, improved stability criteria with a strict dissipative performance index are established. Finally, numerical examples are presented to demonstrate the effectiveness of the proposed control method.
Adaptive Tracking Control Schemes for Fuzzy Approximation-Based Noncanonical Nonlinear Systems with Hysteresis Inputs
Authors: Lai Guanyu , Zeng Kairong , Yang Weijun , Su Xiaohang
Published: July 2023

In this paper, the tracking control problem of a class of fuzzy approximation-based noncanonical nonlinear systems with hysteresis inputs is investigated, where the fuzzy weight matrix is not available for measurement, and the hysteresis nonlinearities are modeled by the Prandtl–Ishlinskii operator. Due to the coupling effects, the plant input containing hysteresis is unknown. To solve the problem, two adaptive control schemes are developed. The first is a Lyapunov-based scheme, and the second is a gradient-based scheme. For convenience, only the relative-degree-one case is taken into account in design and analysis. With the proposed schemes, it can be proved that all signals in the closed-loop system are bounded, and the tracking error converges to a small region around zero. Simulation results show that the maximum steady-state error converges to [−0.0131,0.0183]μm and [−0.0139,0.0161]μm with two control schemes, which confirms the obtained results.
Dynamics of a Predator–Prey Model with Impulsive Diffusion and Transient/Nontransient Impulsive Harvesting
Authors: Quan Qi , Dai Xiangjun , Jiao Jianjun
Published: July 2023

Harvesting is one of the ways for humans to realize economic interests, while unrestricted harvesting will lead to the extinction of populations. This paper proposes a predator–prey model with impulsive diffusion and transient/nontransient impulsive harvesting. In this model, we consider both impulsive harvesting and impulsive diffusion; additionally, predator and prey are harvested simultaneously. First, we obtain the subsystems of the system in prey extinction and predator extinction. We obtain the fixed points of the subsystems by the stroboscopic map theories of impulsive differential equations and analyze their stabilities. Further, we establish the globally asymptotically stable conditions for the prey/predator-extinction periodic solution and the trivial solution of the system, and then the sufficient conditions for the permanence of the system are given. We also perform several numerical simulations to substantiate our results. It is shown that the transient and nontransient impulsive harvesting have strong impacts on the persistence of the predator–prey model.
Dual Protection Routing Trees on Graphs
Authors: Pai Kung-Jui
Published: July 2023

In IP networks, packet forwarding is destination-based and hop-by-hop, and routes are built as needed. Kwong et al. introduced a protection routing in which packet delivery to the destination node can proceed uninterrupted in the event of any single node or link failure. He then showed that “whether there is a protection routing to the destination” is NP-complete. Tapolcai found that two completely independent spanning trees, abbreviated as CISTs, can be used to configure the protection routing. In this paper, we proposed dual protection routing trees, denoted as dual-PRTs to replace CISTs, which are less restrictive than CISTs. Next, we proposed a transformation algorithm that uses dual-PRTs to configure the protection routing. Taking complete graphs Kn, complete bipartite graphs Km,n, hypercubes Qn, and locally twisted cubes LTQn as examples, we provided a recursive method to construct dual-PRTs on them. This article showed that there are no two CISTs on K3,3, Q3, and LTQ3, but there exist dual-PRTs that can be used to configure the protection routing. As shown in the performance evaluation of simulation results, for both Qn and LTQn, we get the average path length of protection routing configured by dual-PRTs is shorter than that by two CISTs.
LPGRI: A Global Relevance-Based Link Prediction Approach for Multiplex Networks
Authors: Wang Chunning , Tang Fengqin , Zhao Xuejing
Published: July 2023

The individuals of real-world networks participate in various types of connections, each forming a layer in multiplex networks. Link prediction is an important problem in multiplex network analysis owing to its wide range of practical applications, such as mining drug targets, recommending friends in social networks, and exploring network evolution mechanisms. A key issue of link prediction within multiplex networks is how to estimate the likelihood of potential links in the predicted layer by leveraging both interlayer and intralayer information. Several studies have shown that incorporating interlayer topological information can improve the performance of link prediction in the predicted layer. Therefore, this paper proposes the Link Prediction based on Global Relevance of Interlayer (LPGRI) method to estimate the likelihood of potential links in the predicted layer of multiplex networks, which comprehensively utilizes both types of information. In the LPGRI method, the contribution of interlayer information is determined using the global relevance (GR) index between layers. Experimental studies on six real multiplex networks demonstrate the competitive performance of our method.
Fuzzy Analytic Network Process with Principal Component Analysis to Establish a Bank Performance Model under the Assumption of Country Risk
Authors: Opreana Alin , Vinerean Simona , Mihaiu Diana Marieta , Barbu Liliana , Șerban Radu-Alexandru
Published: July 2023

In recent years, bank-related decision analysis has reflected a relevant research area due to key factors that affect the operating environment of banks. This study’s aim is to develop a model based on the linkages between the performance of banks and their operating context, determined by country risk. For this aim, we propose a multi-analytic methodology using fuzzy analytic network process (fuzzy-ANP) with principal component analysis (PCA) that extends existing mathematical methodologies and decision-making approaches. This method was examined in two studies. The first study focused on determining a model for country risk assessment based on the data extracted from 172 countries. Considering the first study’s scores, the second study established a bank performance model under the assumption of country risk, based on data from 496 banks. Our findings show the importance of country risk as a relevant bank performance dimension for decision makers in establishing efficient strategies with a positive impact on long-term performance. The study offers various contributions. From a mathematic methodology perspective, this research advances an original approach that integrates fuzzy-ANP with PCA, providing a consistent and unbiased framework that overcomes human judgement. From a business and economic analysis perspective, this research establishes novelty based on the performance evaluation of banks considering the operating country’s risk.
A Quantile Functions-Based Investigation on the Characteristics of Southern African Solar Irradiation Data
Authors: Maposa Daniel , Masache Amon , Mdlongwa Precious
Published: July 2023

Exploration of solar irradiance can greatly assist in understanding how renewable energy can be better harnessed. It helps in establishing the solar irradiance climate in a particular region for effective and efficient harvesting of solar energy. Understanding the climate provides planners, designers and investors in the solar power generation sector with critical information. However, a detailed exploration of these climatic characteristics has not yet been studied for the Southern African data. Very little exploration is being done through the use of measures of centrality only. These descriptive statistics may be misleading. As a result, we overcome limitations in the currently used deterministic models through the application of distributional modelling through quantile functions. Deterministic and stochastic elements in the data were combined and analysed simultaneously when fitting quantile distributional function models. The fitted models were then used to find population means as explorative parameters that consist of both deterministic and stochastic properties of the data. The application of QFs has been shown to be a practical tool and gives more information than approaches that focus separately on either measures of central tendency or empirical distributions. Seasonal effects were detected in the data from the whole region and can be attributed to the cyclical behaviour exhibited. Daily maximum solar irradiation is taking place within two hours of midday and monthly accumulates in summer months. Windhoek is receiving the best daily total mean, while the maximum monthly accumulated total mean is taking place in Durban. Developing separate solar irradiation models for summer and winter is highly recommended. Though robust and rigorous, quantile distributional function modelling enables exploration and understanding of all components of the behaviour of the data being studied. Therefore, a starting base for understanding Southern Africa’s solar climate was developed in this study.
The Anti-Inflammatory and Skin Barrier Function Recovery Effects of Schisandra chinensis in Mice with Atopic Dermatitis
Authors: Son Yoorae , Yang Wonjin , Park Sangjun , Yang Jinkyu , Kim Soyeon , Lyu Ji-Hyo , Kim Hyungwoo
Published: July 2023

Background and Objectives: The fruit of Schisandra chinensis (Turcz.) Baill. is widely used medicinally to treat coughs, asthma, exhaustion, eczema, and pruritus in Northeast Asian countries, including Korea, China, and Japan. This study was designed to investigate the effects of S. chinensis on dermatitis in mice with calcipotriol (MC-903)-induced atopic dermatitis (AD), and its effects on skin barrier dysfunction was also investigated. Materials and Methods: The inhibitory effects of an ethanolic extract of S. chinensis (EESC) on skin lesions, water content, water-holding capacity (WHC), histopathological abnormalities, and inflammatory cytokine and chemokine levels were evaluated in mice with AD induced by MC903. Results: Topical EESC ameliorated skin lesions, reduced skin water content, and increased MC903-induced WHC. EESC also prevented MC-903-induced histopathological abnormalities such as epidermal disruption, hyperkeratosis, spongiotic changes, and immune cell infiltration in inflamed tissue. Moreover, topical EESC reduced MC-903-induced levels of pro-inflammatory cytokines and chemokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-4, IL-6, IL-8, monocyte chemotactic protein (MCP)-1, and thymic stromal lymphopoietin (TSLP). Furthermore, unlike dexamethasone, EESC did not reduce the spleen/body weight ratio. Conclusions: These results suggest that S. chinensis can be used as an alternative to external corticosteroids and that its anti-inflammatory and skin barrier dysfunction-restoring effects are related to the downregulation of pro-inflammatory cytokines and chemokines, such as TNF-α, IL-4, IL-6, IL-8, and TSLP.
Integrated NMR and MS Analysis of the Plasma Metabolome Reveals Major Changes in One-Carbon, Lipid, and Amino Acid Metabolism in Severe and Fatal Cases of COVID-19
Authors: Gama-Almeida Marcos C. , Pinto Gabriela D. A. , Teixeira Lívia , Hottz Eugenio D. , Ivens Paula , Ribeiro Hygor , Garrett Rafael , Torres Alexandre G. , Carneiro Talita I. A. , Barbalho Bianca de O. , Ludwig Christian , Struchiner Claudio J. , Assunção-Miranda Iranaia , Valente Ana Paula C. , Bozza Fernando A. , Bozza Patrícia T. , dos Santos Gilson C. , El-Bacha Tatiana
Published: July 2023

Brazil has the second-highest COVID-19 death rate worldwide, and Rio de Janeiro is among the states with the highest rate in the country. Although vaccine coverage has been achieved, it is anticipated that COVID-19 will transition into an endemic disease. It is concerning that the molecular mechanisms underlying clinical evolution from mild to severe disease, as well as the mechanisms leading to long COVID-19, are not yet fully understood. NMR and MS-based metabolomics were used to identify metabolites associated with COVID-19 pathophysiology and disease outcome. Severe COVID-19 cases (n = 35) were enrolled in two reference centers in Rio de Janeiro within 72 h of ICU admission, alongside 12 non-infected control subjects. COVID-19 patients were grouped into survivors (n = 18) and non-survivors (n = 17). Choline-related metabolites, serine, glycine, and betaine, were reduced in severe COVID-19, indicating dysregulation in methyl donors. Non-survivors had higher levels of creatine/creatinine, 4-hydroxyproline, gluconic acid, and N-acetylserine, indicating liver and kidney dysfunction. Several changes were greater in women; thus, patients’ sex should be considered in pandemic surveillance to achieve better disease stratification and improve outcomes. These metabolic alterations may be useful to monitor organ (dys) function and to understand the pathophysiology of acute and possibly post-acute COVID-19 syndromes.
Research on IMU-Assisted UWB-Based Positioning Algorithm in Underground Coal Mines
Authors: Wang Lei , Zhang Shangqi , Qi Junyan , Chen Hongren , Yuan Ruifu
Published: July 2023

The application of an ultra-wideband (UWB) positioning system in a Global Positioning System (GPS) denial environment such as an underground coal mine, mainly focuses on position information and rarely involves information such as direction attitude. Position accuracy is often affected by multipath, non-visible ranges, base station layout, and more. We proposed an IMU-assisted UWB-based positioning system for the provision of positioning and orientation services to coal miners in underground mines. The Error-State Kalman Filter (ESKF) is used to filter the errors in the measured data from the IMU-assisted UWB positioning system to obtain the best estimate of the error for the current situation and correct for inaccuracies due to approximations. The base station layout of the IMU-assisted UWB positioning system was also simulated. The reasonable setting of the reference base station location can suppress multi-access interference and improve positioning accuracy to a certain extent. Numerous simulation experiments have been conducted in GPS denial environments, such as underground coal mines. The experimental results show the effectiveness of the method for determining the position, direction, and attitude of the coal miner under the mine, which provides a better reference value for positioning and orientation in a GPS rejection environment such as under the mine.
A New Proposed Symbiotic Plant–Herbivore Relationship between Burkea africana Trees, Cirina forda Caterpillars and Their Associated Fungi Pleurostomophora richardsiae and Aspergillus nomius
Authors: Nemadodzi Lufuno Ethel , Prinsloo Gerhard
Published: July 2023

Burkea africana is a tree found in savannah and woodland in southern Africa, as well as northwards into tropical African regions as far as Nigeria and Ethiopia. It is used as fuel wood, medicinally to treat various conditions, such as toothache, headache, migraine, pain, inflammation, and sexually transmitted diseases, such as gonorrhoea, but also an ornamental tree. The current study investigated the possible symbiotic relationship between B. africana trees and the C. forda caterpillars and the mutual role played in ensuring the survival of B. africana trees/seedlings in harsh natural conditions and low-nutrient soils. Deoxyribonucleic acid isolation and sequencing results revealed that the fungal species Pleurostomophora richardsiae was highly predominant in the leaves of B. africana trees and present in the caterpillars. The second most prominent fungal species in the caterpillars was Aspergillus nomius. The latter is known to be related to a Penicillium sp. which was found to be highly prevalent in the soil where B. africana trees grow and is suggested to play a role in enhancing the effective growth of B. africana trees in their natural habitat. To support this, a phylogenetic analysis was conducted, and a tree was constructed, which shows a high percentage similarity between Aspergillus and Penicillium sp. The findings of the study revealed that B. africana trees not only serve as a source of feed for the C. forda caterpillar but benefit from C. forda caterpillars which, after dropping onto the soil, is proposed to inoculate the soil surrounding the trees with the fungus A. nomius which suggests a symbiotic and/or synergistic relationship between B. africana trees and C. forda caterpillars.
Effects of Ion Combinations and Their Concentrations on Denitrification Performance and Gene Expressions of an Aerobic Strain Marinobacter Hydrocarbonoclasticus RAD-2
Authors: Li Junchi , Cai Lei , Lu Huifeng , Ma Bin , Chen Guangsuo , Kong Dedong , Hu Yiming , Ye Ziran , Ruan Yunjie
Published: July 2023

Salinity is one of the most important factors affecting the nitrogen-removal efficiency of denitrifying bacteria. A series of different ion combinations and salinity gradients were carried out to clarify the effects of ion types and concentrations on nitrogen removal by halophilic aerobic denitrifying bacteria RAD-2. Nitrate concentrations, nitrite concentrations, TAN concentrations, and OD600 were monitored to investigate their effects on denitrification in each group. The results showed that Na+, K+, and Cl- accelerated the denitrification process and improved nitrogen-removal efficiency at moderate additions, while Ca2+ and Mg2+ showed no significant effect. Na+ was effective alone, while K+ or Cl- needed to be combined with at least one of Na+, K+, or Cl- to achieve similar efficiency. The batch tests of salinity confirmed that the addition of a moderate concentration of NaCl/Na2SO4 could effectively improve nitrogen-removal efficiency, while excessive salinity might hinder denitrification metabolism. In the salinity range of 5~40‰, a 5‰ dosage might be the most economical method for strain RAD-2. Real-time PCR experiments on 17 key nitrogen metabolism-related genes revealed that chloride was widely involved in the nitrogen and carbon metabolism of microorganisms by altering cell osmotic pressure and opening ion channel proteins, thereby affecting the efficiency of denitrification. The results of this study may contribute to a better understanding of the different roles of various ions in aerobic denitrification and highlight the importance of salinity control in highly salted wastewater treatment.
Whole Genome Sequencing Reveals High Genetic Diversity, Diverse Repertoire of Virulence-Associated Genes and Limited Antibiotic Resistance Genes among Commensal Escherichia coli from Food Animals in Uganda
Authors: Byarugaba Denis K. , Wokorach Godfrey , Alafi Stephen , Erima Bernard , Najjuka Florence , Mworozi Edison A. , Kibuuka Hannah , Wabwire-Mangen Fred
Published: July 2023

Commensal Escherichia coli with broad repertoire of virulence and antimicrobial resistance (AMR) genes pose serious public health risks as reservoirs of AMR and virulence. This study undertook whole genome characterization of commensal E. coli from food-producing animals in Uganda to investigate their genome variability (resistome and virulome). We established that the E. coli had high genomic diversity with 38 sequence types, 24 FimH types, and 33 O-antigen serotypes randomly distributed within three phylogroups (A, B1, and E). A greater proportion (≥93.65%) of the E. coli were resistant to amoxicillin/clavulanate and ampicillin antibiotics. The isolates were AmpC beta-lactamase producers dominated by blaEC-15 (71.88%) and tet(A) (20.31%) antimicrobial resistant genes besides a diverse armory of virulence-associated genes in the class of exotoxin, adhesins, iron uptake, and serine protease autotransporters which varied by host species. Cattle were found to be the major source of E. coli carrying Shiga toxin genes, whereas swine was the main source of E. coli carrying colicin-like Usp toxin gene. The study underscores the importance of livestock as the carrier of E. coli with antimicrobial resistance and a large repertoire of virulence traits with a potential of causing disease in animals and humans by acquiring more genetic traits.
Extracellular Expression of Feruloyl Esterase and Xylanase in Escherichia coli for Ferulic Acid Production from Agricultural Residues
Authors: Lan Jiaxin , Ji Shujie , Yang Chuanjia , Cai Guolin , Lu Jian , Li Xiaomin
Published: July 2023

There is still a large amount of ferulic acid (FA), an outstanding antioxidant, present in agricultural residues. Enzymatic hydrolysis has been regarded as the most effective way to release FA. This present study therefore selected feruloyl esterase (FAE) and xylanase (XYN) from the metagenomes of a cow rumen and a camel rumen, respectively, for their recombinant expression in Escherichia coli BL21 and further application in releasing FA. After screening the candidate signal peptides, the optimal one for each enzyme, which were selected as SP1 and SP4, respectively, was integrated into the vectors pET22b(+) and pETDuet-1. Among the generated E. coli strains SP1-F, SP4-X, and SP1-F-SP4-X that could express extracellular enzymes either separately or simultaneously, the latter one performed the best in relation to degrading the biomass and releasing FA. Under the optimized culture and induction conditions, the strain SP1-F-SP4-X released 90% of FA from 10% of de-starched wheat bran and produced 314.1 mg/L FA, which was deemed to be the highest obtained value to the best of our knowledge. This result could pave a way for the re-utilization of agricultural residues and enhancing their add-value.
Multi-Isotopic Compositions of Ores from the Shizishan Cu–Au–Mo Orefield in the Tongling Region, Eastern China: Implications for Ore Genesis
Authors: Li Jinwei , Pan Lichuan , Guo Yitong , Lu Shunfu
Published: July 2023

The Middle–Lower Yangtze Metallogenic Belt (MLYMB) hosts abundant porphyry–skarn–stratabound-type Cu–Au–Mo deposits. Despite extensive research, the origin of the stratabound-type deposits, which developed at the unconformity interface between the Devonian and Carboniferous strata in the MLYMB, remains controversial. The primary debate centers on whether these deposits are the result of Carboniferous sedimentary exhalative mineralization or Mesozoic magmatic–hydrothermal mineralization. In this paper, we examine three representative deposits in the Shizishan orefield: the Chaoshan skarn-type Au deposit, the Hucun porphyry–skarn-type Cu–Mo deposit, and the Dongguashan Cu–(Au) deposit, which has a disputed genesis of its stratiform orebodies. Economically important ore minerals, such as chalcopyrite, molybdenite, and pyrrhotite, and their associated quartz and calcite, were focused on, rather than the extensively studied pyrite in the Tongling region. The ore genesis and sources of mineralized elements in the Shizishan orefield were investigated using H, O, C, S, Pb, and Cu isotopes. The H–O isotopic compositions of hydrothermal quartz from the Chaoshan, Dongguashan, and Hucun deposits indicate that the ore-forming fluids were mainly magmatic water with some meteoric water input. The C–O isotopic compositions of calcite show a large difference from the local sedimentary carbonates. The S isotopic compositions of sulfides reveal a magmatic sulfur signature. The Pb isotopic compositions in the three deposits are similar to those of the Shizishan intrusions, suggesting a magmatic source for Pb. The Cu isotopic compositions of chalcopyrite and pyrrhotite demonstrate that Cu, the primary ore-forming element, was mainly derived from magmatic–hydrothermal fluids. The stratiform orebodies display H–O–C–S–Pb–Cu isotopes consistent with the porphyry orebodies in the Dongguashan deposit, as well as in the Chaoshan and Hucun deposits, indicating a common ore genesis. From these, we conclude that the porphyry–skarn–stratabound-type Cu–Au–Mo deposits in the Shizishan orefield can be classified as a unified Mesozoic magmatic–hydrothermal metallogenic system. The stratabound-type copper sulfide deposits and the porphyry–skarn-type copper deposits in the MLYMB have a strong similarity in the source and genesis of their ore-forming elements.
Dried Loquat Fruit Extract Containing Chlorogenic Acid Prevents Depressive-like Behaviors Induced by Repeated Corticosteroid Injections in Mice
Authors: Lim Dong Wook , Yoo Guijae , Lee Changho
Published: July 2023

Eriobotrya japonica (loquat tree) has been used in traditional medicine to treat respiratory ailments, inflammation, and skin diseases; however, its potential antidepressant-like effects have not been extensively investigated. In this study, we evaluated the antidepressant-like effects of E. japonica fruit extract (EJFE) in a mouse model of corticosterone (CORT)-induced depression. An HPLC analysis revealed that chlorogenic acid (CGA) is the major compound in EJFE. Male ICR mice (5weeks-old) were injected with CORT (40 mg/kg, intraperitoneally) once daily for 21 days to induce depressive-like behaviors. Various behavioral tests, including the open field test, rotarod test, elevated plus maze (EPM), passive avoidance test (PAT), tail suspension test (TST), and forced swim test (FST), were conducted 1 h after the oral administration of EJFE at different doses (30, 100, and 300 mg/kg) and CGA (30 mg/kg). High-dose EJFE and CGA significantly alleviated CORT-induced depressive-like behaviors, as indicated by the reduced immobility times in the TST and FST. A decrease in the step-through latency time in the PAT, without an effect on locomotor activity, suggested an improvement in cognitive function. Moreover, EJFE- and CGA-treated mice exhibited significantly reduced anxiety-like behaviors in the EPM. Our results imply the promising potential of EJFE containing CGA as a therapeutic candidate for depression.
A Novel Kinetic Modeling of Enzymatic Hydrolysis of Sugarcane Bagasse Pretreated by Hydrothermal and Organosolv Processes
Authors: Moreira Neto João , Costa Josiel Martins , Bonomi Antonio , Costa Aline Carvalho
Published: July 2023

Lignocellulosic biomasses have a complex and compact structure, requiring physical and/or chemical pretreatments to produce glucose before hydrolysis. Mathematical modeling of enzymatic hydrolysis highlights the interactions between cellulases and cellulose, evaluating the factors contributing to reactor scale-up and conversion rates. Furthermore, this study evaluated the influence of two pretreatments (hydrothermal and organosolv) on the kinetics of enzymatic hydrolysis of sugarcane bagasse. The kinetic parameters of the model were estimated using the Pikaia genetic algorithm with data from the experimental profiles of cellulose, cellobiose, glucose, and xylose. The model considered the phenomenon of non-productive adsorption of cellulase on lignin and inhibition of cellulase by xylose. Moreover, it included the behavior of cellulase adsorption on the substrate throughout hydrolysis and kinetic equations for obtaining xylose from xylanase-catalyzed hydrolysis of xylan. The model for both pretreatments was experimentally validated with bagasse concentration at 10% w/v. The Plackett–Burman design identified 17 kinetic parameters as significant in the behavior of process variables. In this way, the modeling and parameter estimation methodology obtained a good fit from the experimental data and a more comprehensive model.
Reduced Graphene Oxide Embedded with ZnS Nanoparticles as Catalytic Cathodic Material for Li-S Batteries
Authors: Colombo Roberto , Versaci Daniele , Amici Julia , Bella Federico , Para Maria Laura , Garino Nadia , Laurenti Marco , Bodoardo Silvia , Francia Carlotta
Published: July 2023

Lithium-sulfur technology is a strong candidate for the future generation of batteries due to its high specific capacity (1675 mAh g−1), low cost, and environmental impact. In this work, we propose a facile and solvent-free microwave synthesis for a composite material based on doped (sulfur and nitrogen) reduced graphene oxide embedded with zinc sulfide nanoparticles (SN-rGO/ZnS) to improve the battery performance. The chemical-physical characterization (XRD, XPS, FESEM, TGA) confirmed the effectiveness of the microwave approach in synthesizing the composite materials and their ability to be loaded with sulfur. The materials were then thoroughly characterized from an electrochemical point of view (cyclic voltammetry, galvanostatic cycling, Tafel plot, electrochemical impedance spectroscopy, and Li2S deposition test); the SN-rGO/ZnS/S8 cathode showed a strong affinity towards polysulfides, thus reducing their loss by diffusion and improving redox kinetics, allowing for faster LiPSs conversion. In terms of performance, the composite-based cathode increased the specific capacity at high rate (1 C) from 517 to 648 mAh g−1. At the same time, more stable behavior was observed at 0.5 C with capacity retention at the 750th cycle, where it was raised from 32.5% to 48.2%, thus confirming the beneficial effect of the heteroatomic doping process and the presence of zinc sulfide nanoparticles.
3D Imaging of Striatal Transplants in a Small Animal Model of Huntington’s Disease
Authors: Schültke Elisabeth , Pinzer Bernd R. , Stampanoni Marco , Harsan Laura , Döbrössy Mátè
Published: July 2023

High-resolution imaging in small animal models of neurologic disease is a technical challenge. In a pilot project, we have explored a non-destructive synchrotron imaging technique for the 3D visualization of intracerebral tissue transplants in a well-established small animal model of Huntington’s disease. Four adult female Sprague Dawley rats each received injections of 0.12 M quinolinic acid (QA) into two target positions in the left striatum, thus creating unilateral left-sided striatal lesions similar to those frequently seen in patients suffering from Huntington’s disease. One week after lesioning, the animals received transplants prepared from whole ganglionic eminences (wGEs) obtained from 13- to 14-day-old rat embryos. Of the four lesioned animals, three received transplants of GNP-loaded cells and one animal received a transplant of naïve cells, serving as control. Post-mortem synchrotron-based microCT was used to obtain images of the neurotransplants. The images obtained of GNP-loaded tissue transplants at the synchrotron corresponded in size and shape to the histological images of transplants developed from naïve cells. Thus, we conclude that non-destructive synchrotron imaging techniques such as phase-contrast imaging are suitable to obtain high-resolution images of GNP-loaded tissue transplants.
Liposomal Epigallocatechin-3-Gallate for the Treatment of Intestinal Dysbiosis in Children with Autism Spectrum Disorder: A Comprehensive Review
Authors: de la Rubia Ortí Jose Enrique , Moneti Costanza , Serrano-Ballesteros Pilar , Castellano Gloria , Bayona-Babiloni Raquel , Carriquí-Suárez Ana Belén , Motos-Muñoz María , Proaño Belén , Benlloch María
Published: July 2023

Autism Spectrum Disorder (ASD) is characterized by varying degrees of difficulty in social interaction and communication. These deficits are often associated with gastrointestinal symptoms, indicating alterations in both intestinal microbiota composition and metabolic activities. The intestinal microbiota influences the function and development of the nervous system. In individuals with ASD, there is an increase in bacterial genera such as Clostridium, as well as species involved in the synthesis of branched-chain amino acids (BCAA) like Prevotella copri. Conversely, decreased amounts of Akkermansia muciniphila and Bifidobacterium spp. are observed. Epigallocatechin-3-gallate (EGCG) is one of the polyphenols with the greatest beneficial activity on microbial growth, and its consumption is associated with reduced psychological distress. Therefore, the objective of this review is to analyze how EGCG and its metabolites can improve the microbial dysbiosis present in ASD and its impact on the pathology. The analysis reveals that EGCG inhibits the growth of pathogenic bacteria like Clostridium perfringens and Clostridium difficile. Moreover, it increases the abundance of Bifidobacterium spp. and Akkermansia spp. As a result, EGCG demonstrates efficacy in increasing the production of metabolites involved in maintaining epithelial integrity and improving brain function. This identifies EGCG as highly promising for complementary treatment in ASD.
Correlations between Nutritional Status and Quality of Life of People with Parkinson’s Disease
Authors: Fernandez Raissa Dias , Bezerra Graziela Maria Benevenuto , Krejcová Lane Viana , Gomes Daniela Lopes
Published: July 2023

Parkinson’s disease (PD) is a neurodegenerative condition that can impact the nutritional status, and such impact seems to be related to the quality of life (QoL). Objective: To evaluate the correlation between anthropometric variables and the QoL of people with Parkinson’s disease (PD). Methods: This is a cross-sectional, descriptive, and analytical study, carried out through the collection of anthropometric data and application of the Parkinson’s Disease Questionnaire PDQ-39. Results: 33 individuals (23 male) diagnosed with PD participated in the research, with a mean age of 58.9 ± 11.6 years. We observed overweight in 45.4% of participants. The perception of QoL showed lower scores for the subjects in the dimensions of body discomfort (75.3 ± 16.6), social support (62.7 ± 15.7), and mobility (61.0 ± 23.6). The correlation between the total QoL score and age (model 1, B = 0.347; CI 0.004–0.902; p = 0.048), which remained statistically significant in the multiple linear regression, regardless of gender (model 2, B = 0.365; CI 0.016–0.937; p = 0.043) and BMI (model 3, B = 0.363; CI 0.006–0.943; p = 0.047), suggests that, in the participants of this study, this relationship does not depend on gender and nutritional status. Conclusion: The perception of QoL was worse in the dimensions of body discomfort, social support, and mobility, worsening with advanced age. Correlations between the worst scores in QoL dimensions and nutritional status were observed. A positive correlation was also identified between age and overall PDQ-39 score, regardless of gender and nutritional status.
An Okinawan-Based Nordic Diet Leads to Profound Effects on Gut Microbiota and Plasma Metabolites Linked to Glucose and Lipid Metabolism
Authors: Manoharan Lokeshwaran , Roth Bodil , Bang Corinna , Stenlund Hans , Ohlsson Bodil
Published: July 2023

Dietary interventions modify gut microbiota and clinical outcomes. Weight reduction and improved glucose and lipid homeostasis were observed after adopting an Okinawan-based Nordic diet (O-BN) in individuals with type 2 diabetes. The aim of the present study was to explore changes in metabolomics and gut microbiota during O-BN and correlate changes with clinical outcomes. A total of 30 patients (17 women), aged 57.5 ± 8.2 years, diabetes duration 10.4 ± 7.6 years, 90% over-weight, were included. Participants were provided an O-BN for 12 weeks. Before and after intervention, and 16 weeks afterwards, anthropometry and clinical data were estimated and questionnaires were collected, as well as samples of blood and stool. Plasma metabolomics were determined by gas- (GC-MS) or liquid- (LC-MS) chromatography-based mass spectrometry and fecal microbiota determination was based on 16S rRNA amplicons from regions V1–V2. During the intervention, weight (6.8%), waist circumference (6.1%), and levels of glucose, HbA1c, insulin, triglycerides, and cholesterol were decreased. Of 602 metabolites, 323 were changed for any or both periods; 199 (101 lipids) metabolites were decreased while 58 (43 lipids) metabolites were increased during the intervention. Changes in glucose homeostasis were linked to changes in, e.g., 1,5-anhydroglucitol, thyroxine, and chiro-inositol. Changes of microbe beta diversity correlated positively with food components and negatively with IL-18 (p = 0.045). Abundance differences at phylum and genus levels were found. Abundances of Actinobacteria, Bacteroidetes, Firmicutes, and Verrucomicrobia correlated with anthropometry, HbA1c, lipids, inflammation, and food. Changes in metabolites and microbiota were reversed after the intervention. The O-BN-induced changes in metabolomics and gut microbiota correspond to clinical outcomes of reduced weight and inflammation and improved glucose and lipid metabolism.
A Randomized, Double-Blind, Controlled Trial Assessing If Medium-Chain Triglycerides in Combination with Moderate-Intensity Exercise Increase Muscle Strength in Healthy Middle-Aged and Older Adults
Authors: Kojima Keiichi , Ishikawa Haruna , Watanabe Shinji , Nosaka Naohisa , Mutoh Tatsushi
Published: July 2023

An adequate nutritional intake is recommended for the prevention of physical frailty and sarcopenia. In particular, medium-chain fatty acids (MCFAs) are reportedly important for muscle strength in nursing home residents. However, the effects of MCFAs on healthy adults at risk for frailty remain unknown. Hence, a randomized, placebo-controlled study was conducted to investigate the effects of 12 weeks of medium-chain triglycerides (MCTs) intake and walking on muscle mass and function in healthy, sedentary, middle-aged and older adults with a low body mass index. Three MCT intake groups with different amounts of octanoic and decanoic acid intake were compared with a control group. After 12 weeks, knee extension strength increased in all groups, with the increases in all MCT intake groups being significantly higher than those in the control group (p < 0.05). Grip strength significantly increased from baseline in the MCT 6 g/day intake group (p < 0.05). The combination of aerobic exercise and MCT intake may be effective in preventing decline in muscle strength and promoting increase in muscle strength as they can improve muscle energy production, thereby contributing to the maintenance of good health for middle-aged and older adults at high risk for frailty and sarcopenia.
Shear-Viscosity-Dependent Effect of a Gum-Based Thickening Product on the Safety of Swallowing in Older Patients with Severe Oropharyngeal Dysphagia
Authors: Bolivar-Prados Mireia , Hayakawa Yuki , Tomsen Noemi , Arreola Viridiana , Nascimento Weslania , Riera Stephanie , Kawakami Satomi , Miyaji Kazuhiro , Takeda Yasuhiro , Kayashita Jun , Clavé Pere
Published: July 2023

Fluid thickening is a valid therapeutic strategy for patients with oropharyngeal dysphagia (OD). The main aim of this study was to determine the therapeutic effect of the xanthan-gum-based thickener Tsururinko Quickly (TQ, Morinaga Milk Co., Tokyo, Japan) in older patients with severe OD. A total of 85 patients (83.32 ± 6.75 y) with OD and a penetration–aspiration score (PAS) of n ≥ 3 were studied by videofluoroscopy while swallowing duplicate 10 mL boluses at <50 mPa·s, 100, 200, 400, 800, and 1600 mPa·s, to assess the safety and efficacy of swallowing and the biomechanics of a swallowing response at each viscosity level. At <50 mPa·s, only 16.25% patients swallowed safely, 45% had penetrations (PAS 3–5), and 38.75% had aspirations (PAS 6–8). Fluid thickening with TQ greatly increased the prevalence of patients with safe swallowing from 62.90% at 100 mPa·s to 95.24% at 1600 mPa·s in a shear-viscosity-dependent manner. The penetrations and aspirations were significantly reduced to 3.60% and 1.19%, respectively, at 1600 mPa·s. The threshold viscosity was 100 mPa·s and the increasing viscosity above 800 mPa·s did not further improve the therapeutic effect significantly. Increasing the shear viscosity significantly reduced the time to laryngeal vestibule closure (−16.70%), increased the time to upper oesophageal sphincter opening (+26.88%), and reduced the pharyngeal bolus velocity (−31.62%) without affecting the pharyngeal residue. TQ has a strong shear-viscosity-dependent effect on the safety of swallowing in older patients with severe OD without increasing the pharyngeal residue. The therapeutic range for TQ is 100–800 mPa·s, with 200 and 800 mPa·s being the optimal doses to cover the needs of older patients with OD.
Micronutrient and Nutritional Status of HIV-Exposed and HIV-Unexposed Malawian Infants in the First Year of Life: Assessment of Ferritin, Vitamin A, and D Status and Its Association with Growth
Authors: Floridia Marco , Galluzzo Clementina Maria , Orlando Stefano , Luhanga Richard , Mphwere Robert , Kavalo Thom , Andreotti Mauro , Amici Roberta , Ciccacci Fausto , Marazzi Maria Cristina , Giuliano Marina
Published: July 2023

Breastfed Malawian infants from Human Immunodeficiency Virus (HIV)-uninfected and HIV-infected women who received antiretroviral therapy were followed until 12 months of age, allowing us to evaluate plasma levels of ferritin, vitamin A (as retinol-binding protein, RBP), and vitamin D (25(OH)D) at six months, as well as nutritional status and growth between six and 12 months. Ferritin and RBP levels were adjusted for inflammation. The study included 88 infants, 63 of whom were part of a recent cohort (2019–2021) that included 49 HIV-exposed but uninfected (HEU) and 14 HIV-unexposed and uninfected (HUU) infants, as well as 25 infants (all HEU) from an earlier cohort (2008–2011). No differences were observed between HEU and HUU infants regarding micronutrient levels, anthropometric indexes, growth, and rates of stunting, being underweight, or wasting. HEU infants from the earlier cohort, when compared to more recent HEU infants, had significantly worse anthropometric measures at six months and inferior growth between six and twelve months. Overall, ferritin deficiency involved 68.6% of infants, while vitamin A and vitamin D deficiency involved 8% and 1.2% of infants, respectively. Micronutrient deficiencies were not associated with HIV exposure, cohort, stunting, being underweight, or wasting. At six months, stunting, being underweight, and wasting involved 25.0%, 2.7% and 2.8% of infants, respectively, with no differences related to HIV exposure. Ferritin deficiency at six months was associated with inferior subsequent growth. In this small observational study conducted in Malawian infants, no major nutritional gap was observed between HIV-exposed and HIV-unexposed infants, though the study highlighted specific nutritional deficiencies that deserve attention. High rates of stunting and ferritin deficiency were observed in the first year of life in Malawian infants, irrespective of maternal HIV status; a significant association between ferritin deficiency and worse subsequent growth was found. Vitamin A and vitamin D deficiencies were much less frequent. Based on the data observed, nutritional interventions should give priority to the correction of ferritin deficiency and chronic undernutrition.