bluuebunny/crossref_metadata_2025 huggingface dataset:
Differential Effects of Organic Ameliorants on the Reassembly of Bacterial Communities in Newly Amended Coastal Mudflat Salt-Affected Soil
Authors: Li Yunlong , Wang Yimin , Gu Chuanhui , Shen Chao , Xu Lu , Zhao Yilin , Yi Siqiang , Zuo Wengang , Shan Yuhua , Zhang Zhuqing , Bai Yanchao
Published: October 2022

Understanding the influences of organic ameliorants amendment on the soil microbiome is fundamental for the alleviation of environmental constraints in coastal mudflat salt-affected soils. However, how the compositional and structural diversity of the microbial community responds to different organic inputs, and key environmental determinants and relevant mechanisms driving soil microbiome reassembly in coastal agroecosystems have not been illustrated. In this study, field experiments were conducted to investigate the effectiveness and underlying mechanisms of sewage sludge (S) and sludge-based vermicompost (V) at different application amounts (0, 50, 100, and 200 t ha−1) on variations in the compositional and structural diversities of soil bacterial community in coastal mudflats. The underlying driving factors, including soil physicochemical and enzymatic properties, were determined to estimate their effects on soil bacterial community. Results show that both types and amounts of organic ameliorants exerted significant influence on the alterations of bacterial community diversities. Particularly, restructured bacterial communities with significantly higher bacterial populations, lower Shannon diversities, and distinct core and unique community profiles were observed in organic ameliorant-amended soils as compared to CK. The co-occurrence networks of bacterial core OTUs reveal that V exhibited more profound effects than those of S on the scale and interactions enhancement and stability reduction of soil bacterial guilds. Additionally, both S and V significantly alleviated environmental constraints with lower pH and salinity, but higher soil organic carbon (SOC) amounts and enzyme activities were observed in the amended soils. Moreover, the effects of S and V on soil amendment were enhanced with the increase in the application amounts of organic ameliorants. Redundancy analysis (RDA) reveals that environmental factors (e.g., pH, salinity, SOC, sucrase, urease, and phosphatase) were pivotal determinants, accounting for 78.05% of the total bacterial community variations in coastal salt-affected soils across different treatments.
Yield Gaps of Major Cereal and Grain Legume Crops in Ethiopia: A Review
Authors: Belachew Kiflemariam Yehuala , Maina Ndegwa Henry , Dersseh Waga Mazengia , Zeleke Bantalem , Stoddard Frederick L.
Published: October 2022

In Ethiopia, smallholder farmers are responsible for most food production. Though yield levels in grain crops have improved greatly over the years, they are still much lower than their potential. The source of yield improvements and the causes of those yield gaps are not well understood. To explain the drivers of yield gaps and current sources of yield improvements in four major cereals (teff, maize, wheat, and sorghum) and three grain legumes (faba bean, common bean, and soybean), we accessed the databases of the Global Yield Gap Atlas, the Food and Agriculture Organization of the United Nations, and the Central Statistical Agency of Ethiopia. Refereed journal articles and grey literature were sought in online databases using keywords. The results showed large increases in production of grain crops with little or no increase in areas of production. The yield increases were primarily attributed to genetic gain rather than agronomic improvements. Farmers’ yields remain far lower than those from on-farm trials and on-station trials and the calculated water-limited yield potential. Currently, yields of wheat, maize, sorghum, and common bean in Ethiopia are about 26.8, 19.7, 29.3, and 35.5% of their water-limited yield potentials. Significant portions of the yield gaps stem from low adoption and use of improved varieties, low application of inputs, continual usage of un-optimized crop management practices, and uncontrolled biotic and abiotic stresses. Proper application of fertilizers and use of improved varieties increase yield by 2 to 3 fold and 24–160%, respectively. Cereal-legume intercropping and crop rotation practices increase yield while reducing severity of pests and the need for application of synthetic fertilizers. In contrast, abiotic stresses cause yield reductions of 20–100%. Hence, dissection of the water-limited yield gap in terms of technology, resource, and efficiency yield gaps will allow the prioritization of the most effective intervention areas.
Oral and Parenteral Vaccination against Escherichia coli in Piglets Results in Different Responses
Authors: Ramis Guillermo , Pérez-Esteruelas Lorena , Gómez-Cabrera Carolina G. , de Pascual-Monreal Clara , Gonzalez-Guijarro Belén , Párraga-Ros Ester , Sánchez-Uribe Pedro , Claver-Mateos Miguel , Mendonça-Pascoal Livia , Martínez-Alarcón Laura
Published: October 2022

The available E. coli vaccines involve two main types (inactivated and live non-pathogenic) and two routes of administration (oral and parenteral) but the mechanism by which both vaccines and routes of administration work is not yet fully elucidated. The influence of a parenteral vaccine (PV) and an oral one (OV) was studied by analyzing the gene expression of biomarkers indicating cellular infiltration (calprotectin, CAL), tight junction proteins (occludin OCL, and zonulin ZON) that maintain intestinal paracellular integration and two proinflammatory (IFN-γ) and anti-inflammatory (TGF-β) mediator cytokines, as well as histomorphology and IgA production cell density. Differences were observed in CAL, more infiltrated in orally vaccinated animals; OCL also increased in orally vaccinated animals, and higher density of IgA-producing cells in ileum for orally vaccinated groups. Cytokine expression is also different; and there is a lower mRNA for IFN-γ in the parenteral than in the oral vaccinated animals. Finally, the villus height-to-crypt depth ratio was higher in the orally vaccinated groups. The data collectively show clear and different effects derived from the use of each type of vaccine, route of administration and regimen. The results suggest a more rapid and direct effect of oral vaccination and a state of suppression in the absence of a second oral stimulus by the pathogen.
Hypocholesterolemic, Antioxidative, and Anti-Inflammatory Effects of Dietary Spirulina platensisis Supplementation on Laying Hens Exposed to Cyclic Heat Stress
Authors: Al-Otaibi Morshed I. M. , Abdellatif Hasan A. E. , Al-Huwail Abdelmohsen K. A. , Abbas Ahmed O. , Mehaisen Gamal M. K. , Moustafa Eman S.
Published: October 2022

This study aimed to investigate the role of dietary Spirulina platensis (SP) supplementation in relieving the negative impacts of heat stress (HS) on the productive performance, cholesterol profile, redox status, and inflammatory cytokines of laying hens. A total of 288, 45-wk-old and 1550.7 ± 2.3 g initial body weight, HY-Line W-36 laying hens were housed in two environmental-controlled compartments. Layers were allotted to eight treatments of a two × four factorial design, with six replicates containing six birds per treatment. The temperature in one of the compartments was kept at a thermoneutral condition (24 °C group), while the temperature in the other compartment was raised to a cyclic heat stress of 35 °C from 9:00 a.m. to 5.00 p.m. (35 °C group). Layers in each compartment were fed on one of four experimental diets, containing 0%, 3%, 6%, or 9% SP (SP groups). The trial continued for five weeks. As a result of this study, exposure of laying hens to cyclic HS resulted in a significant (p < 0.05) increase in the total cholesterol (CH), low-density lipoprotein-CH, liver- and egg yolk-CH, ceruloplasmin, malondialdehyde, interleukins (IL-1β and IL-6), and tumor necrosis factor-α, and a significant (p < 0.05) decrease in the high-density lipoprotein-CH, total antioxidant capacity, and reduced glutathione levels. HS negatively (p < 0.05) affected the hen–day egg production (EP, 90.5% vs. 77.0%), egg weight (EW, 61.8 g vs. 56.8 g), feed intake (FI, 111.6 g vs. 101.5 g) and feed conversion ratio (FCR, 2.00 vs. 2.37). As SP levels increased in layer diets, a linear (p < 0.05) improvement response in most of the parameters was obtained in both HS and non-HS layers, recording the best results with 9% SP (e.g., 78.8% vs. 87.6% EP, 56.7 g vs. 61.9 g EW, 103.3 g vs. 110.2 g FI, and 2.38 vs. 2.04 FCR, in 0% vs. 9% SP, respectively). When incorporating SP into the diets of HS-layers, the negative impacts of HS were remarkably relieved (p < 0.05). Therefore, diets containing 9% SP could be used as a promising approach to improve the productive and physiological performance of laying hens, particularly under heat stress conditions.
Mass Spectrometry-Based Lipidomics of Brown Adipose Tissue and Plasma of New-Born Lambs Subjected to Short-Term Cold Exposure
Authors: Graña-Baumgartner Andrea , Dukkipati Venkata S. R. , Biggs Patrick J. , Kenyon Paul R. , Blair Hugh T. , López-Villalobos Nicolás , Ross Alastair B.
Published: October 2022

During cold exposure, brown adipose tissue (BAT) holds the key mechanism in the generation of heat, thus inducing thermogenic adaptation in response to cooler environmental changes. This process can lead to a major lipidome remodelling in BAT, where the increase in abundance of many lipid classes plays a significant role in the thermogenic mechanisms for heat production. This study aimed to identify different types of lipids, through liquid chromatography–mass spectrometry (LC-MS), in BAT and plasma during a short-term cold challenge (2-days), or not, in new-born lambs. Fifteen new-born Romney lambs were selected randomly and divided into three groups: Group 1 (n = 3) with BAT and plasma obtained within 24 h after birth, as a control; Group 2 (n = 6) kept indoors for two days at an ambient temperature (20–22 °C) and Group 3 (n = 6) kept indoors for two days at a cold temperature (4 °C). Significant differences in lipid composition of many lipid categories (such as glycerolipids, glycerophospholipids, sphingolipids and sterol lipids) were observed in BAT and plasma under cold conditions, compared with ambient conditions. Data obtained from the present study suggest that short-term cold exposure induces profound changes in BAT and plasma lipidome composition of new-born lambs, which may enhance lipid metabolism via BAT thermogenic activation and adipocyte survival during cold adaptation. Further analysis on the roles of these lipid changes, validation of potential biomarkers for BAT activity, such as LPC 18:1 and PC 35:6, should contribute to the improvement of new-born lamb survival. Collectively, these observations help broaden the knowledge on the variations of lipid composition during cold exposure.
The Mineral Composition of Bone Marrow, Plasma, Bones and the First Antlers of Farmed Fallow Deer
Authors: Steiner-Bogdaszewska Żaneta , Tajchman Katarzyna , Ukalska-Jaruga Aleksandra , Florek Mariusz , Pecio Monika
Published: October 2022

An adequate supply of essential nutrients is particularly important during the skeletal growth and development of young deer, especially in males, who build new antlers each year. The aim of the research was to analyze the levels of 21 mineral elements (including the bulk elements: Ca, P, Mg, K, Na; trace elements: Li, Cr, Mn, Co, Cu, Zn, Se, Mo; and toxic elements: Be, Al, As, Cd, Sb, Ba, Pb, Ni) in the bone marrow, plasma, bones, and first antlers of farmed fallow deer (Dama dama). The mineral compositions of tissues were analyzed using inductively coupled plasma mass spectrometry. Higher concentrations of Ca, P, Mg, Cr, Zn, Se, Al, Ba and Ni were found in bone marrow than in plasma. The highest concentrations of Ca, P and Ba were recorded in fallow deer bone, while the highest concentrations of Mg, K, Na, Li, Cr, Mn, Co, Cu, Zn, Se, Mo, Be, Al, As, Sb, Pb and Ni were found in the antlers. Moreover, the research showed a significant negative relationship between Ca and Cd, and between Ca and Pb, and P and Pb (rS = −0.70, rS = −0.80, and rS = −0.66, respectively; p < 0.05) in the tissues.
Impacts of Dam Age on Lifetime Productivity of Angus Replacement Beef Females
Authors: Wellnitz Krista R. , Parsons Cory T. , Dafoe Julia M. , Boss Darrin L. , Wyffels Samuel A. , DelCurto Timothy , Van Emon Megan L.
Published: October 2022

Comprehensive cow-calf production data was utilized to evaluate the impact of dam age on lifetime productivity of Angus replacement beef females. Cows used in this study were commercial Angus replacement females born between 2006 and 2020, ranging in age from 1 to 14-yr of age (n = 3568). To determine the impact of dam age on lifetime productivity, cows were classified by age groups, specifically 2-, 3-, 4-, 5-, 6/7-, and 8-yrs old and older. The 8-yr and older group consisted of females that were up to 14-yr of age. Cow BW at breeding exhibited a cow age × dam age interaction (p < 0.01). Cows at 5-yrs of age from 2-yr old dams weighed less at breeding than cows at 5-yrs of age from 3-, 4-, 5- and 8-years and older dams, with cows at 5-yr of age from 6/7-yr old dams being intermediate. The probability of remaining in the herd at the age of 5 was significant for dam age (p = 0.05) averaging 69.41%, but after further delineation, no significant impacts of dam age were observed. Productivity as a measure of total pounds of calf weaned through 5-yrs displayed a dam age effect (p = 0.01) with cows from 8-yrs or older dams weaning more total pounds of calf, than cows from 3-yr-old dams. In summary, the impact of dam age on lifetime productivity indicates that dam age can impact future productivity of their offspring.
Contrasting Fecal Methanogenic and Bacterial Profiles of Organic Dairy Cows Located in Northwest Washington Receiving Either a Mixed Diet of Pasture and TMR or Solely TMR
Authors: Slanzon Giovana , Sischo William , McConnel Craig
Published: October 2022

Currently, little is known regarding fecal microbial populations and their associations with methanogenic archaea in pasture-based dairy cattle. In this study, we assessed the fecal microbiome of organic dairy cows across different time points receiving a mixed diet of pasture and total mixed ration (TMR) or TMR only. We hypothesized that the fecal methanogenic community, as well as co-occurrence patterns with bacteria, change across diets. To test these hypotheses, we analyzed TMR and pasture samples, as well as the V3-V4 region of 16S rRNA of fecal samples collected over the course of a one-year study period from 209 cows located on an organic dairy in Northwest Washington. The inherent variability in pasture quality, quantity, availability, and animal preference can lead to diverse dietary intakes. Therefore, we conducted a k-means clustering analysis to identify samples from cows that were associated with either a pasture-based diet or a solely TMR diet. A total of 4 clusters were identified. Clusters 1 and 3 were mainly associated with samples primarily collected from cows with access to pasture of varying quality and TMR, cluster 2 was formed by samples from cows receiving only TMR, and cluster 4 was a mix of samples from cows receiving high-quality pasture and TMR or TMR only. Interestingly, we found little difference in the relative abundance of methanogens between the community clusters. There was evidence of differences in diversity between pasture associated bacterial communities and those associated with TMR. Cluster 4 had higher diversity and a less robust co-occurrence network based on Spearman correlations than communities representing TMR only or lower-quality pasture samples. These findings indicate that varied bacterial communities are correlated with the metabolic characteristics of different diets. The overall good pasture and TMR quality in this study, combined with the organic allowance for feeding high levels of TMR even during the grazing season, might have contributed to the lack of differences in the fecal archaeal community from samples associated with a mixed pasture and TMR diet, and a TMR only diet. Mitigation strategies to decrease methane emissions such as increasing concentrate to forage ratio, decreasing pasture maturity and adopting grazing systems targeting high quality pasture have been shown to be efficient for pasture-based systems. However, the allowance for organic dairy producers to provide up to an average of 70% of a ruminant’s dry matter demand from dry matter fed (e.g., TMR), suggests that reducing enteric methane emissions may require the development of novel dietary strategies independent of pasture management.
New Occurrences of the Tiger Shark (Galeocerdo cuvier) (Carcharhinidae) off the Coast of Rio de Janeiro, Southeastern Brazil: Seasonality Indications
Authors: Aximoff Izar , Cumplido Rodrigo , Rodrigues Marcelo Tardelli , de Melo Ubirajara Gonçalves , Fagundes Netto Eduardo Barros , Santos Sérgio Ricardo , Hauser-Davis Rachel Ann
Published: October 2022

The tiger shark Galeocerdo cuvier (Péron & Lesueur, 1822) (Carcharhinidae) is classified as near-threatened along the Brazilian coast, in line with its global categorization. Although Rio de Janeiro, located in southeastern Brazil, is internationally identified as a priority shark conservation area, many shark species, including tiger sharks, are landed by both industrial and artisanal fisheries in this state. However, there is a lack of detailed information on the species capture pressures and records for the state of Rio de Janeiro. Therefore, the aims of this study were to expand the tiger shark record database and to improve upon future conservation and management strategies. Tiger shark records from four coastal Rio de Janeiro regions were obtained by direct observation. The information obtained from fishery colonies/associations, environmental guards, researchers, and scientific articles, totaling 23 records, resulted in an approximately 5-fold increase in the number of tiger shark records off the coast of the state of Rio de Janeiro. A possible seasonality pattern concerning the size of the captured/observed animals was noted, emphasizing the need to consider the coast of Rio de Janeiro as an especially relevant area for at least part of the life history of tiger sharks.
Suspected Frostbite Injuries in Coypu (Myocastor coypus)
Authors: Gethöffer Friederike , Gregor Katharina M. , Zdora Isabel , Wohlsein Peter , Schöttes Franziska , Siebert Ursula
Published: October 2022

Native to South America, the coypu (Myocastor coypus) is an invasive alien species (IAS) of Union concern. It was introduced to Germany a hundred years ago and is considered established in all German federal states. Between January and February 2021, ground temperatures below −10° Celsius were recorded in Lower Saxony, Germany, for approximately two consecutive weeks. Five male and five female coypus, harvested between 23 February and 31 March 2021, received a post-mortem examination. Nutritional status was poor in six cases, moderate in three and good in one case. Pregnancy was observed in two females. In all the animals, lesions were predominantly found on the distal limbs (n = 7) and/or tail (n = 10), involving the skin and soft tissue with occasional exposure or loss of bones. The histological findings consisted of chronic, ulcerative to necrotizing dermatitis and occasional ulcerative-suppurative dermatitis, necrotizing myositis, thrombosis, granulation tissue, fibrosis and intralesional dystrophic mineralization. Intralesional bacteria were present in six and fungal spores in one animal. Determination of the exact cause was not possible; however, considering the local weather conditions and the distribution of lesions, frostbite injuries have to be considered as the most likely cause. The intralesional bacteria and fungal spores most likely represent secondary contaminants. Interestingly, lesions of this kind have not been reported in coypus in Germany so far. Therefore, frostbite should be considered as a potential cause of disease in coypus, warranting further investigation.
Efficacy of Five Disinfectant Products Commonly Used in Pig Herds against a Panel of Bacteria Sensitive and Resistant to Selected Antimicrobials
Authors: Montagnin Clara , Cawthraw Shaun , Ring Isaac , Ostanello Fabio , Smith Richard P. , Davies Rob , Martelli Francesca
Published: October 2022

The growing threat of antimicrobial resistance worldwide has led to an increasing concern in the human, veterinary, and environmental fields, highlighting the need for strategies to effectively control bacterial contamination. Correct biosecurity practices, including the appropriate use of disinfectants, play a crucial role in controlling bacterial contamination. This study aimed to verify whether the recommended concentrations defined according to the Defra General Orders concentration (GO, published by the UK Department for Environment, Food and Rural Affairs’ disinfectant-approval scheme) of five commercial disinfectant preparations (peroxygen-based, phenol-based, two halogen-releasing agents, and glutaraldehyde/quaternary ammonium compound-based; disinfectants A to E, respectively) were sufficient to inhibit growth and inactivate selected bacterial strains, including some that carry known phenotypic patterns of multidrug resistance. The effectiveness of each disinfectant was expressed as the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values, determined by the broth-microdilution method. The results indicate that the type of disinfectant and its concentration influence the inhibitory and bactericidal efficacy. The glutaraldehyde/quaternary ammonium compound-based (disinfectant D) and chlorocresol-based products (disinfectant B) were the most effective, and the GO concentration was bactericidal in all the strains tested. The efficacy of the other compounds varied, depending on the bacterial species tested. The GO concentrations were at least able to inhibit the bacterial growth in all the products and bacterial strains tested. A greater tolerance to the compounds was observed in the strains of E. coli with multidrug-resistance profiles compared to the strains that were sensitive to the same antimicrobials.
Embryo, Relocation and Secondary Nests of the Invasive Species Vespa velutina in Galicia (NW Spain)
Authors: Diéguez-Antón Ana , Escuredo Olga , Seijo María Carmen , Rodríguez-Flores María Shantal
Published: October 2022

Invasive species become established in non-native areas due to their intrinsic characteristics and the ability to adapt to new environments. This work describes the characteristics of the nesting behavior of the invasive yellow-legged hornet (Vespa velutina nigrithorax) in Galicia (Northwest Spain). The first nest was detected in the area in 2012 and after that, the distribution pattern shows a species-invasion curve with slow progress at first but followed by rapid expansion. The nesting places for this hornet differ between the kinds of nests, while embryo nests are mainly found in buildings in spring, secondary nests are observed in vegetation in summer, autumn, and winter. The annual life cycle starts when the queen builds the embryo nests and starts to lay eggs. This leads to the emergence of the first workers, usually small in size, and sometimes a few males. After this stage, large nests called secondary nests are normally observed in most exposed sites. Relocation nests can also be observed; these are nests in the first stage of development presenting adults insects but without brood or meconium. The period of decline is characterized by the emergence of new queens and males, that are distinguishable even in the pupal stage, the appearance of two eggs per cell, and an irregular brood pattern.
Prevalence of Primary Radiographic Signs of Hip Dysplasia in Dogs
Authors: Pinna Stefania , Tassani Chiara , Antonino Alessandro , Vezzoni Aldo
Published: October 2022

Hip dysplasia is a disabling orthopedic disease in dogs. The aim of this retrospective study was to evaluate the morphological aspects and radiographic changes in the development of hip dysplasia in adult dogs, and to describe the frequency of each radiographic parameter according to each Fédération Cynologique Internationale (FCI) grade. Brass descriptive forms for the evaluation of hip dysplasia were obtained from the archive of the Italian Fondazione Salute Animale, and the radiographic evaluation of 642 hips were processed. Sixteen radiographic criteria were assessed, divided into six main parameters: acetabulum, femoral head and its position in the acetabulum, femoral neck, joint space, and Norberg angle. The initial mild alterations were shown in the craniolateral acetabular rim (31.8%), slightly divergent in the joint space in 58.6% of FCI-A. The spherical shape of the femoral head was mildly small/flattened in 56.9% of FCI-B, in addition to a slightly cylindrical-shaped femoral neck (60.5%) and slightly lost contours (55.0%). Changes in acetabular depth (45.0%), and in the cranial acetabular margin (56.7%) were found in FCI-C. The center of the femoral head was lateral to the dorsal acetabular rim in 70.0% of FCI-B; the Norberg angle appeared normal in 70.6% of FCI-B. Elaboration of the radiographic criteria from the Brass descriptive forms allowed for the extrapolation of accurate knowledge regarding morphologic changes in the development of dysplasia by providing detailed information for each individual. In particular, the present survey showed that the morphological alterations of the acetabulum prevailed over those of the femoral head only at the beginning of the development of canine hip dysplasia, and then worsened after the changes occurred in the femoral head and neck.
Glycogen Synthase Kinase 3β (GSK3β) Regulates Myogenic Differentiation in Skeletal Muscle Satellite Cells of Sheep
Authors: Yang Jingquan , Yang Haosen , Wang Linjie , Zhou Ping
Published: October 2022

Glycogen synthase kinase 3β (GSK3β) has a vital role in the regulation of many cellular processes. However, the role of GSK3β in muscle cell differentiation in sheep remains unknown. In this study, we investigated the function of GSK3β in skeletal muscle satellite cells (SMSCs) of sheep. An overexpression of GSK3β significantly inhibited myotube formation as well as the mRNA levels of myogenic genes (MyoD, MyoG, MyHC1, and MyHC2a) in sheep SMSCs. SB216763 treatment had a time-course effect on the phosphorylation levels of sheep GSK3β. In addition, reducing the activity of GSK3β lead to the promotion of sheep SMSCs differentiation as well as the mRNA levels of myogenic genes (MyoD, MyoG, MyHC1, and MyHC2a). This study illustrated the function of GSK3β to inhibit myogenesis in sheep SMSCs, which provided evidence for studying the mechanisms involved in the regulation of sheep SMSCs differentiation by GSK3β.
The Use of Removed Mesquite Brush as a Fiber Replacement in Silage Production
Authors: Fair William Taylor , Breeden Jeffrey Bryan , Atchley Thomas Wayne , Lambert Barry Don , Aljoe Zhan , Owsley Walter Franklin , Smith William Brandon
Published: October 2022

Mesquite (Prosopis L.) is considered an invasive browse species in most of the American Southwest. Mechanical intervention removes yields an excess of organic debris. Anecdotal evidence in the past has supported using such browse as feed for livestock. Thus, our objectives were to (1) determine the nutritive value and fermentation characteristics of silage produced with mesquite biomass, and (2) evaluate solvent treatment of mesquite biomass prior to ensiling. In Experiment 1, we evaluated mesquite inclusion rate (0, 250, 500, 750, or 1000 g kg−1 DM), length of fermentation (28, 56, or 84 d), and silage inoculant. In Experiment 2, we evaluated the effects of mesquite pre-treatment with acid (H2SO4) or alkali (NaOH) solutions. Concentrations of NDF, ADF, and ADL, as well as IVTD, decreased (p < 0.05) with increasing mesquite inclusion. However, 250 g mesquite kg−1 DM did not differ from grass silage. There was no effect (p > 0.05) of inoculation, though increasing length of incubation did increase (p < 0.05) VFA production and decrease (p < 0.05) silage pH. Solvent treatment did not improve ensiling properties. Results are interpreted to mean that mesquite biomass may be effectively incorporated into silage at levels up to 250 g kg−1.
miR-183/96/182 Cluster Regulates the Development of Bovine Myoblasts through Targeting FoxO1
Authors: Ru Wenxiu , Liu Kunpeng , Yang Jiameng , Liu Jianyong , Qi Xinglei , Huang Bizhi , Chen Hong
Published: October 2022

Muscle development is an important factor affecting meat yield and quality and is coordinated by a variety of the myogenic genes and signaling pathways. Recent studies reported that miRNA, a class of highly conserved small noncoding RNA, is actively involved in regulating muscle development, but many miRNAs still need to be further explored. Here, we identified that the miR-183/96/182 cluster exhibited higher expression in bovine embryonic muscle; meanwhile, it widely existed in other organizations. Functionally, the results of the RT-qPCR, EdU, CCK8 and immunofluorescence assays demonstrated that the miR-183/96/182 cluster promoted proliferation and differentiation of bovine myoblast. Next, we found that the miR-183/96/182 cluster targeted FoxO1 and restrained its expression. Meanwhile, the expression of FoxO1 had a negative correlation with the expression of the miR-183/96/182 cluster during myoblast differentiation. In a word, our findings indicated that the miR-183/96/182 cluster serves as a positive regulator in the proliferation and differentiation of bovine myoblasts through suppressing the expression of FoxO1.
Efficacy of Antimicrobial Agents in Dentifrices: A Systematic Review
Authors: Marinho Vanessa Teixeira , dos Reis Andréa Cândido , da Costa Valente Mariana Lima
Published: October 2022

The aim of this systematic review was to verify if the presence of different antimicrobial agents in dentifrices is effective in reducing the number of microorganisms for disease prevention. This review followed the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) guidelines and was registered with the Open Science Framework (OSF). A search was conducted in the PubMed, Embase, Scopus, and Web of Science databases. Two independent authors reviewed the titles and abstracts according to the inclusion criteria, which comprised in vitro studies published in English that evaluated the efficacy of antimicrobial agents in dentifrices and their antimicrobial activity. A total of 527 articles were found. Of these, 334 were included for reading of the title and abstract, and 69 were selected for reading in full. In the end, 39 articles remained in this review. Triclosan, sodium fluoride, and sodium monofluorophosphate were the most commonly used chemical antimicrobial agents. Among the herbal agents, miswak extract and neem extract were the most commonly used. The presence of antimicrobial agents in dentifrice formulations can promote the reduction of the number of microorganisms involved in oral diseases, but with variations in their effectiveness, depending on the agent used and the microorganism evaluated.
A Seven-Year Microbiological and Molecular Study of Bacteremias Due to Carbapenemase-Producing Klebsiella Pneumoniae: An Interrupted Time-Series Analysis of Changes in the Carbapenemase Gene’s Distribution after Introduction of Ceftazidime/Avibactam
Authors: Papadimitriou-Olivgeris Matthaios , Bartzavali Christina , Karachalias Eleftherios , Spiliopoulou Anastasia , Tsiata Ekaterini , Siakallis Georgios , Assimakopoulos Stelios F. , Kolonitsiou Fevronia , Marangos Markos
Published: October 2022

Background: Ceftazidime/avibactam (CZA) is a new option for the treatment of KPC-producing Klebsiella pneumoniae. The aim of this study was to determine resistance patterns and carbapenemase genes among K. pneumoniae (CP-Kp) bacteremic isolates before and after CZA introduction. Methods: K. pneumoniae from blood cultures of patients being treated in a Greek university hospital during 2015–21 were included. PCR for blaKPC, blaVIM, blaNDM and blaOXA-48 genes was performed. Results: Among 912 K. pneumoniae bacteremias: 725 (79.5%) were due to carbapenemase-producing isolates; 488 (67.3%) carried blaKPC; 108 (14.9%) blaVIM; 100 (13.8%) blaNDM; and 29 (4%) carried a combination of blaKPC, blaVIM or blaNDM. The incidence of CP-Kp bacteremias was 59 per 100,000 patient-days. The incidence of CP-Kp changed from a downward pre-CZA trend to an upward trend in the CZA period (p = 0.007). BSIs due to KPC-producing isolates showed a continuous downward trend in the pre-CZA and CZA periods (p = 0.067), while BSIs due to isolates carrying blaVIM or blaNDM changed from a downward trend in the pre-CZA to an upward trend in the CZA period (p < 0.001). Conclusions: An abrupt change in the epidemiology of CP-Kp was observed in 2018, due to the re-emergence of VIM-producing isolates after the suppression of KPC-producing ones via the use of CZA.
Genomic Analysis of a Hybrid Enteroaggregative Hemorrhagic Escherichia coli O181:H4 Strain Causing Colitis with Hemolytic-Uremic Syndrome
Authors: Kislichkina Angelina A. , Kartsev Nikolay N. , Skryabin Yury P. , Sizova Angelika A. , Kanashenko Maria E. , Teymurazov Marat G. , Kuzina Ekaterina S. , Bogun Alexander G. , Fursova Nadezhda K. , Svetoch Edward A. , Dyatlov Ivan A.
Published: October 2022

Hybrid diarrheagenic E. coli strains combining genetic markers belonging to different pathotypes have emerged worldwide and have been reported as a public health concern. The most well-known hybrid strain of enteroaggregative hemorrhagic E. coli is E. coli O104:H4 strain, which was an agent of a serious outbreak of acute gastroenteritis and hemolytic uremic syndrome (HUS) in Germany in 2011. A case of intestinal infection with HUS in St. Petersburg (Russian Federation) occurred in July 2018. E. coli strain SCPM-O-B-9427 was obtained from the rectal swab of the patient with HUS. It was determined as O181:H4-, stx2-, and aggR-positive and belonged to the phylogenetic group B2. The complete genome assembly of the strain SCPM-O-B-9427 contained one chromosome and five plasmids, including the plasmid coding an aggregative adherence fimbriae I. MLST analysis showed that the strain SCPM-O-B-9427 belonged to ST678, and like E. coli O104:H4 strains, 2011C-3493 caused the German outbreak in 2011, and 2009EL-2050 was isolated in the Republic of Georgia in 2009. Comparison of three strains showed almost the same structure of their chromosomes: the plasmids pAA and the stx2a phages are very similar, but they have distinct sets of the plasmids and some unique regions in the chromosomes.
Effects of Copper Exposure on Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress, Autophagy and Immune Response in Different Tissues of Chinese Mitten Crab (Eriocheir sinensis)
Authors: Feng Wenrong , Su Shengyan , Song Changyou , Yu Fan , Zhou Jun , Li Jianlin , Jia Rui , Xu Pao , Tang Yongkai
Published: October 2022

High concentrations of copper (Cu2+) pose a great threat to aquatic animals. However, the mechanisms underlying the response of crustaceans to Cu2+ exposure have not been well studied. Therefore, we investigated the alterations of physiological and molecular parameters in Chinese mitten crab (Eriocheir sinensis) after Cu2+ exposure. The crabs were exposed to 0 (control), 0.04, 0.18, and 0.70 mg/L of Cu2+ for 5 days, and the hemolymph, hepatopancreas, gills, and muscle were sampled. The results showed that Cu2+ exposure decreased the antioxidative capacity and promoted lipid peroxidation in different tissues. Apoptosis was induced by Cu2+ exposure, and this activation was associated with the mitochondrial and ERK pathways in the hepatopancreas. ER stress-related genes were upregulated in the hepatopancreas but downregulated in the gills at higher doses of Cu2+. Autophagy was considerably influenced by Cu2+ exposure, as evidenced by the upregulation of autophagy-related genes in the hepatopancreas and gills. Cu2+ exposure also caused an immune response in different tissues, especially the hepatopancreas, where the TLR2-MyD88-NF-κB pathway was initiated to mediate the inflammatory response. Overall, our results suggest that Cu2+ exposure induces oxidative stress, ER stress, apoptosis, autophagy, and immune response in E. sinensis, and the toxicity may be implicated following the activation of the ERK, AMPK, and TLR2-MyD88-NF-κB pathways.
Soy Isoflavones Protect Neuronal PC12 Cells against Hypoxic Damage through Nrf2 Activation and Suppression of p38 MAPK and AKT–mTOR Pathways
Authors: Zhang Yongzhu , Yin Liqing , Dong Jiajia , Xia Xiudong
Published: October 2022

Isoflavones are a class of major phenolic compounds, derived from soybeans, that possess unique therapeutic and biological properties. The possible mechanisms of isoflavone-mediated protection of neuronal PC12 cells against hypoxic damage was investigated in this study. Isoflavones showed potential neuroprotective effects by increasing cell viability, decreasing the level of reactive oxygen species (ROS), and inhibiting apoptosis and cell cycle arrest in cobalt chloride (CoCl2)-induced hypoxic damage. A Western blot analysis indicated that isoflavones decreased apoptosis by up-regulating the Bcl-xL protein and down-regulating the Bax protein. They further reduced the S-phase fraction of the cell cycle by down-regulating the p21 protein and up-regulating the cyclin A protein levels. Additionally, isoflavones activated Nrf2 protein translocation and inhibited the p38 MAPK and AKT–mTOR pathways. A molecular docking analysis further revealed that isoflavones displayed a potential competitive interaction with the Nrf2 protein for Keap1. Our findings suggest that isoflavones could be a potent neuroprotective phytochemical in soybeans and their products.
NOX as a Therapeutic Target in Liver Disease
Authors: Matuz-Mares Deyamira , Vázquez-Meza Héctor , Vilchis-Landeros María Magdalena
Published: October 2022

The nicotinamide adenine dinucleotide phosphate hydrogen oxidase (NADPH oxidase or NOX) plays a critical role in the inflammatory response and fibrosis in several organs such as the lungs, pancreas, kidney, liver, and heart. In the liver, NOXs contribute, through the generation of reactive oxygen species (ROS), to hepatic fibrosis by acting through multiple pathways, including hepatic stellate cell activation, proliferation, survival, and migration of hepatic stellate cells; hepatocyte apoptosis, enhancement of fibrogenic mediators, and mediation of an inflammatory cascade in both Kupffer cells and hepatic stellate cells. ROS are overwhelmingly produced during malignant transformation and hepatic carcinogenesis (HCC), creating an oxidative microenvironment that can cause different and various types of cellular stress, including DNA damage, ER stress, cell death of damaged hepatocytes, and oxidative stress. NOX1, NOX2, and NOX4, members of the NADPH oxidase family, have been linked to the production of ROS in the liver. This review will analyze some diseases related to an increase in oxidative stress and its relationship with the NOX family, as well as discuss some therapies proposed to slow down or control the disease’s progression.
Ultrasonic Enhancement of Aqueous Two-Phase Extraction and Acid Hydrolysis of Flavonoids from Malvaviscus arboreus Cav. Flower for Evaluation of Antioxidant Activity
Authors: Yuan Tiefeng , Huang Jilong , Gan Lin , Chen Linzhou , Zhong Jinjian , Liu Zhaohan , Wang Liping , Fan Huajun
Published: October 2022

The ultrasonic-assisted aqueous two-phase extraction (UAATPE) of flavonoid glycosides from Malvaviscus arboreous Cav. flower (MACF) was developed using ethanol/ammonia sulfate systems, followed by the ultrasonic-assisted acid hydrolysis (UAAH) of the top extract with HCl solution. The optimization of UAATPE and UAAH processes was accomplished by single-factor experiments and response surface methodology. As a result, the flavonoid glycosides enriched in the top phase could achieve a maximum yield of 35.9 ± 1.1 mg/g by UAATPE and were completely hydrolyzed by UAAH deglycosylation. The flavonoid glycosides and their hydrolyzates were separated and characterized by high-performance liquid chromatography and ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. Ultrasonic enhancement of the extraction and hydrolysis was explored by comparative study. Furthermore, the in vitro activity of the flavonoid glycosides and the aglycones were comprehensively evaluated by antioxidant activity assays, including ferric-reducing antioxidant power and scavenging DPPH, hydroxyl, and superoxide radicals. All of the IC50 values suggest that the antioxidant activity of flavonoid aglycones was stronger than that of their glucosides and even vitamin C, revealing that the deglycosylated flavonoids from MACF were the more powerful antioxidants. This study provided an effective and eco-friendly strategy for the extraction, separation, and purification of flavonoids from MACF, as well as for the development of the potential flavonoid antioxidants.
Special Issue on Image Processing Techniques for Biomedical Applications
Authors: Di Ruberto Cecilia , Loddo Andrea , Putzu Lorenzo
Published: October 2022

In recent years, there has been growing interest in creating powerful biomedical image processing tools to assist medical specialists [...]
Experimental and Numerical Investigations of Dynamic Failure Mechanisms of Underground Roadway Induced by Incident Stress Wave
Authors: He Zhi-Long , Lu Cai-Ping , Zhang Xiu-Feng
Published: October 2022

The mechanisms of dynamic disasters around underground roadways/tunnels were examined by adopting split Hopkinson pressure bar (SHPB) laboratory tests to reproduce the failure process of the surrounding rock subjected to incident stress waves. On the basis of ensuring the consistency of numerical simulations with the experimental results, the failure mechanisms of the surrounding rock and spatiotemporal evolution of the hoop stress around the hole were studied by using a two-dimensional particle flow code (PFC2D). The results of the numerical simulation indicate that tensile stress and compressive stress concentrate along the horizontal and vertical directions around the hole, respectively, owing to the instantaneous incidence of compressive stress waves. The failure modes of surrounding rocks are significantly different when the hole is subjected to various intensities of incident stress waves. In addition, the stability of the surrounding rock of the hole is greatly affected by the amplitude and wavelength of the incident wave and the elastic modulus of the surrounding rock.
A New Mechanical Specific Energy Model for Composite Impact Drilling
Authors: Qin Jianyu , Yin Siyuan , Yang Naitong , Chen Xuyue , Tian Bo , Xue Liang , Ma Yi
Published: October 2022

Composite impact drilling technology is one of the important techniques to increase drilling speeds in deep hard formations. In order to evaluate the efficiency of drilling with a composite impactor in real time and effectively, and to further improve the drilling speed in deep formations, the mechanical specific energy (MSE) model of drilling with a composite impactor was studied. Based on previous theoretical studies on MSE, and considering the effect of the composite impactor on the axial and torsional impact of the drill bit during operation, a new MSE model for composite percussion drilling was established. The results show that the evaluation of the drilling efficiency by MSE obtained by this calculation model is consistent with the actual situation of a Lufeng X well. It can be more widely used in wells drilled with composite impact tools and meet the needs of field operations.
Enhancing Reliability Analysis with Multisource Data: Mitigating Adverse Selection Problems in Bridge Monitoring and Management
Authors: Sinha Ananta , Chorzepa Mi G. , Yang Jidong J. , Kim S. Sonny , Durham Stephan
Published: October 2022

Data collected using sensors plays an essential role in active bridge health monitoring. When analyzing a large number of bridges in the U.S., the National Bridge Inventory data as been widely used. Yet, the database does not provide information about live loads, one of the most indeterminate variables for monitoring bridges. Such asymmetric information can lead to an adverse selection problem in making maintenance, rehabilitation, and repair decisions. This study proposes a data-driven reliability analysis to assess probabilities of bridge failure by synthesizing NBI data and Weigh-In-Motion (WIM) data for a large number of bridges in Georgia. On the resistance side, tree ensemble methods are employed to support the hypothesis that the NBI operating load rating represents the distribution of bridge resistance capacities which change over time. On the loading side, the live load distribution is derived from field data collected using WIM sensors. Our results show that the proposed WIM data-enabled reliability analysis substantially enhances information symmetry and provides a reliability index that supports monitoring of bridge conditions, depending on live loads and load-carrying capacities.
Buckling Analysis of a Composite Honeycomb Reinforced Sandwich Embedded with Viscoelastic Damping Material
Authors: Qi Dezhong , Sun Qiang , Zhang Sanqiang , Wang Yuanfang , Zhou Xiaoqiang
Published: October 2022

In this study, the buckling loads of a composite sandwich structure, which is reinforced by a honeycomb layer and filled with viscoelastic damping material, are analyzed. By applying von Karman anisotropic plate equations for large deflection, the governing equation of the composite sandwich structure is determined, and the deflection of the structure is further defined by a double triangular series. According to the dynamic equivalent effective stiffness obtained by the homogenous asymptotic method and Hill’s generalized self-consistent model based on the Halpin–Tsai model, limiting the dynamic load buckling of the composite honeycomb reinforced sandwich structure embedded with viscoelastic damping material under axial compression can be achieved. The factors that influence the composite sandwich’s buckling loads are discussed and compared, such as the load and geometry parameters, the thickness of the honeycomb reinforcement layer and the honeycomb’s width. Finally, the results obtained by the present method are validated by the existing literature.
Automatic Detection of Construction Workers’ Helmet Wear Based on Lightweight Deep Learning
Authors: Liang Han , Seo Suyoung
Published: October 2022

To reduce the risk of head trauma to workers working in high-risk workplaces such as construction sites, we designed a new automated lightweight end-to-end convolutional neural network to identify whether all people on a construction site are wearing helmets. Firstly, we used GhostNet as the backbone feature extraction network to take advantage of its low running cost and make the model lighter overall while ensuring efficient automatic feature extraction. Secondly, we designed a multi-scale segmentation and feature fusion network (MSFFN) in the feature-processing stage to improve the algorithm’s robustness in detecting objects at different scales. In contrast, the design of the feature fusion network can enrich the diversity of helmet features and improve the accuracy of helmet detection when distance changes, viewpoint changes, and occlusion phenomena occur. Thirdly, we proposed an improved version of the attention mechanism, the lightweight residual convolutional attention network version 2 (LRCA-Netv2). The main idea of the improvement is implemented around the spatial dimension by fusing the combined features along with the horizontal and vertical directions and then weighting them separately. Such an operation allows the establishment of dependencies between the more distant features with improved accuracy compared to the original LRCA-Net. Finally, when tested on the dataset, the proposed lightweight helmet-wearing detection network has a mAP and FPS of 93.5% and 42.