bluuebunny/crossref_metadata_2025 huggingface dataset:
The Co-Occurrence of Physiological and Epicotyl Physiological Dormancy in Three Desiccation-Sensitive Castanopsis (Fagaceae) Acorns from China with Specific Reference to the Embryonic Axis Position
Authors: Li Jiajin , Jaganathan Ganesh K. , Kang Han , Liu Baolin
Published: November 2023

Ecological significance of dormancy in desiccation-sensitive seeds is poorly understood. Quercus exhibits mutually exclusive occurrence of physiological (PD) and epicotyl dormancy (ePD), with no reported co-occurrence or dormancy class in other genera. We aimed to understand the dormancy in three Castanopsis species and document desiccation sensitivity and germination patterns concerning the embryonic axis position. We hypothesized that Castanopsis acorns are recalcitrant and potentially dormant. Fresh and cold-stratified acorns of Castanopsis chinensis, Castanopsis purpurella, and Castanopsis sclerophylla were subjected to desiccation and germination. Seedling emergence and internal morphology was monitored following cold (CS) and warm (WS) stratification. Fresh acorns had radicles emerge only after CS but require WS for shoot emergence. Drying to 20% moisture content led to complete death. In C. purpurella and C. sclerophylla, the embryonic axis was near the scar, and germination occurred by cracking the pericarp near the scar, which contrasts with C. chinensis. Moderate drying relieved dormancy due to the mechanical resistance of the pericarp. All three acorns were desiccation-sensitive and dormant. This is the first explicit report on PD and ePD co-occurrence in desiccation-sensitive seeds, but literature surveys allow for inference of such coexistence. CS alleviated PD and WS relieved ePD. Winter temperatures break PD, and acorns germinate during spring, but shoot emergence is delayed until summer. Our results are instructive for research on the dormancy of desiccation-sensitive species and the reproduction of Fagaceae species in subtropical forests.
Greece’s Forest Sector from the Perspective of Timber Production: Evolution or Decline?
Authors: Koulelis Panagiotis P. , Tsiaras Stefanos , Andreopoulou Zacharoula S.
Published: November 2023

Greece’s trade patterns in wood and wood products align with the broader trade dynamics in the Mediterranean region, where wood and wood products are primarily imported. Using Pettitt’s test, this study revealed a consistent decline in the production of selected wood and wood product categories in Greece, coupled with a noteworthy increase in imports. Following the economic crisis of 2008, a temporary dip in import trends in some categories was observed. Furthermore, Pettitt’s test helped identify the specific years when significant shifts occurred in the time series data. In the latter part of the analysis, the study underscored the significance of various factors within the wood removal production sector, including urbanization, economic growth, personal income, and the labor force. National imports of goods and wood fuel were also identified as playing pivotal roles at the national level. Recognizing the link between economic indicators and wood removals has the potential to inform decisions related to sustainable resource management, conservation initiatives, and strategies for economic development.
Blackwellomyces kaihuaensis and Metarhizium putuoense (Hypocreales), Two New Entomogenous Fungi from Subtropical Forests in Zhejiang Province, Eastern China
Authors: Li Yi , Zhao Xin-Chang , Wu Li-Xia , Wang Yu , Xu Ao , Lin Wen-Fei
Published: November 2023

Entomogenous fungi are one of the most diverse fungal groups among ascomycetes. During investigations of macro-fungi biodiversity in Kaihua and Putuo in Zhejiang Province, China, in 2021, several entomopathogenic fungal specimens were collected from subtropical forests. After a careful examination of morphology and multi-gene phylogenetic analyses applying nrSSU, nrLSU, tef1, rpb1 and rpb2, two novel species were discovered, described herein as Blackwellomyces kaihuaensis and Metarhizium putuoense, and further compared with other similar species. Detailed descriptions, color photographs of fresh specimen and figures illustrating microscopic features of the two new species are provided.
Individual Fish Echo Detection Method Based on Peak Delay Estimation and Instantaneous Frequency Characterization
Authors: Yang Hang , Cheng Jing , Li Guodong , Tang Taolin , Chen Jun
Published: November 2023

In fisheries science research and farmed fish monitoring, acquiring individual fish echoes is the basis for the growth assessment, tracking, and target strength estimation of farmed fish. However, individual fish echo detection methods based on narrowband signal features cannot be applied well to high-density aquaculture scenarios. The broadband signaling system can improve the distance resolution of the detected target and can help to improve the performance of individual fish echo detection. In this study, for the broadband signal system and the characteristics of the underwater fish acoustic echoes, an individual fish echo detection method is proposed using the matched filter output envelope peak interval and instantaneous frequency characteristics of the echo as evaluation indices, and the simulation and experiments of the method are carried out in an anechoic water tank. The results show that the broadband signal system and the corresponding detection method perform better in detecting single target echoes than the narrowband signal system. Compared with the broadband single echo detection method that only relies on the peak interval of the matched filter envelope, the joint detection method that incorporates the instantaneous frequency characteristics of the echo signal has a better rejection capability for overlapping echoes, which can reduce the probability of misjudging the overlapping echoes. The combined detection methods may provide a better detection performance for individual fish echoes.
Numerical Investigation of Critical Hydraulic Parameters Using FLOW-3D: A Case Study of Taunsa Barrage, Pakistan
Authors: Zaffar Muhammad Waqas , Haasan Ishtiaq , Ghumman Abdul Razzaq
Published: November 2023

Hydraulic structures, such as barrages, play an important role in the sustainable development of several regions worldwide. The aim of this novel study is to identify the critical hydraulic parameters (CHPs) of Taunsa Barrage, built on the Indus River. These CHPs, including free surface profiles, flow depths, Froude number, velocity profiles, energy dissipation and turbulence kinetic energy, were investigated using simulation via FLOW-3D numerical models. Incompressible Reynolds-averaged Navier–Stokes (RANS) equations on each computational cell were solved using the numerical methods available in FLOW-3D. The simulation results indicated that the locations of hydraulic jumps (HJs) were lower than that were reported in the previous one-dimensional study. Similarly, the distances of the HJs from the downstream toe of the glacis were reached at 2.97 m and 6 m at 129.10 m and 130.30 m tailwater levels, respectively, which deviated from the previous studies. In higher tailwater, the sequent depth ratio also deviated from the previous data. The maximum turbulent kinetic energies were observed in the developing regions of HJs, which were found to be decreased as the distance from the HJ was increased. The results of this research will be highly useful for engineers working in the field of design of hydraulic structures.
Addition of Silver Nanoparticles to Composite Edible Films and Coatings to Enhance Their Antimicrobial Activity and Application to Cherry Preservation
Authors: Bizymis Angelos-Panagiotis , Kalantzi Styliani , Mamma Diomi , Tzia Constantina
Published: November 2023

The aim of this study was to examine the potential enhancement of the antimicrobial activity of edible films, composed of (i) chitosan (CH), cellulose nanocrystals (CNC) and beta-cyclodextrin (CD) (50%-37.5%-12.5%) and (ii) hydroxypropyl methylcellulose (HPMC), cellulose nanocrystals (CNC) and beta-cyclodextrin (CD) (50%-37.5%-12.5%), with silver nanoparticle (AgNP) incorporationat levels 5, 10 and 15% v/v. According to the results, the AgNP addition led to very high antimicrobial activity of both films, reducing by more than 96% the microbial growth of the Gram-negative bacterium Escherichia coli (E. coli) in all cases. On the other hand, by adding AgNPs to films, their thickness as well as oxygen and water vapor permeability decreased, while their transparency increased. Furthermore, the contribution of these specific edible films to preserve cherries under cold storage was investigated. All edible coatings resulted in an improvement of the fruit properties under consideration, and especially the color difference, hardness and total microbial load.
Specificity Testing for NGT PCR-Based Detection Methods in the Context of the EU GMO Regulations
Authors: Zanatta Caroline Bedin , Hoepers Aline Martins , Nodari Rubens Onofre , Agapito-Tenfen Sarah Zanon
Published: November 2023

The term new genomic techniques (NGTs) is an umbrella term used to describe a variety of techniques that can alter the genetic material of an organism and that have emerged or have been developed since 2001, when the existing genetically modified organism (GMO) legislation was adopted. The analytical framework used to detect GMOs in Europe is an established single harmonized procedure that is mandatory for the authorization of GM food and feed, thus generating a reliable, transparent, and effective labeling scheme for GMO products. However, NGT products can challenge the implementation and enforcement of the current regulatory system in the EU, relating in particular to the detection of NGT products that contain no foreign genetic material. Consequently, the current detection methods might fail to meet the minimum performance requirements. Although existing detection methods may be able to detect and quantify even small alterations in the genome, this does not necessarily confirm the distinction between products resulting from NGTs subject to the GMO legislation and other products. Therefore, this study provides a stepwise approach for the in silico prediction of PCR systems’ specificity by testing a bioinformatics pipeline for amplicon and primer set searches in current genomic databases. In addition, it also empirically tested the PCR system evaluated during the in silico analysis. Two mutant genotypes produced by CRISPR-Cas9 in Arabidopsis thaliana were used as a case study. Overall, our results demonstrate that the single PCR system developed for identifying a nucleotide insertion in the grf1-3 genotype has multiple matches in the databases, which do not enable the discrimination of this mutated event. Empirical assays further support this demonstration. In contrast, the second mutated genotype, grf8-61, which contains a -3 bp deletion, did not yield any matches in the sequence variant database. However, the primer sequences were not efficient during the empirical assay. Our approach represents a first step in decision making for analytical methods for NGT detection, identification, and quantification in light of the European labeling regulations.
Prevalence of Salmonella in Eggs from Conventional and Cage-Free Egg Production Systems and the Role of Consumers in Reducing Household Contamination
Authors: Solís Doina , Cordero Ninoska , Quezada-Reyes Maritza , Escobar-Astete Carla , Toro Magaly , Navarrete Paola , Reyes-Jara Angélica
Published: November 2023

Salmonella is one of the leading causes of foodborne disease worldwide, usually related to contaminated poultry or poultry products, such as eggs. Since egg contamination with Salmonella depends on multiple factors that make it challenging to control, consumers’ knowledge about food safety and the proper handling of eggs is crucial. The aims of the study were (1) to determine the prevalence of Salmonella in eggs from conventional and alternative production systems, (2) to characterize the Salmonella isolates according to phenotypic-genotypic and antimicrobial-resistant traits, and (3) to understand how consumers manage the hazards related to egg contamination in the household. A total of 426 egg samples were analyzed (conventional systems = 240; alternative systems = 186). Culture-based and molecular microbiological methods were used to identify Salmonella and bioinformatics analysis of whole genome sequences was used to determine the serotype and antimicrobial-resistant genes. Salmonella enterica serotype Enteritidis was detected only in eggs from alternative systems (1.1%, 2/186). Isolates showed resistance to nalidixic acid (100%, 2/2), and the aac(6′)-Iaa gene and a mutation in the gyrA gene were identified in both isolates. Overall, consumers demonstrated knowledge regarding food safety; however, many still engage in practices that pose a risk of acquiring foodborne illnesses.
Association of Adverse Childhood Experiences with Non-Suicidal Self-Injury and Suicidality: Baseline Survey of the Chinese Adolescent Health Growth Cohort
Authors: Guo Shuangshuang , Jiao Ting , Ma Ying , Lewis Stephen P. , Ammerman Brooke A. , Chen Ruoling , Thomas Erica , Yu Yizhen , Tang Jie
Published: November 2023

Many studies have identified that adverse childhood experiences (ACEs) are associated with non-suicidal self-injury (NSSI) and suicidality. However, most studies have been restricted to a few types of ACEs. This study aims to investigate the association of 13 common types of ACEs with NSSI, suicidal ideation (SI), and suicide attempts (SA), as well as the mediation of depressive and anxiety symptoms therein. A total of 1771 (994 male, 777 female) students aged 11–16 (12.9 ± 0.6) years who participated in the baseline survey of the Chinese Adolescent Health Growth Cohort study were included in the analysis. ACEs, including childhood maltreatment, other common forms of ACEs, and smoking, were measured via the Chinese version of the Child Trauma Questionnaire (CTQ) and a series of valid questionnaires that were derived from previous studies. NSSI was measured using the Chinese version of the Functional Assessment of Self-mutilation. SI and SA were measured using questions derived from the Global School Based Student Health Survey. Depressive symptoms were measured via the Chinese version of the Center for Epidemiologic Studies Depression Scale, and anxiety symptoms were measured via the General Anxiety Disorder-7. Of the included participants, 92.0% reported one or more category of ACEs. Smoking, parent–child separation, emotional abuse, physical abuse, and being bullied were positively associated with NSSI; smoking, parent–child separation, emotional abuse, physical abuse, emotional neglect, and being bullied were positively associated with SI; smoking, emotional abuse, and being bullied were positively associated with SA. The associations of ACEs with NSSI, SI, and SA were each partially or completely mediated through depressive and anxiety symptoms. Children and adolescents who had experiences of smoking, physical abuse, and being bullied during childhood are consistently and independently associated with NSSI and suicidality, and these associations may be largely mediated through depressive and anxiety symptoms. In conclusion, not all the types of ACEs are independently associated with NSSI, and suicidality and other associations may mediate through depressive and anxiety symptoms. Target interventions for adolescents’ NSSI and suicidality should focus on those who have a history of ACEs and depressive and anxiety symptoms.
Family Dynamics in Colonial La Rioja: A Case Analysis of Five Generations
Authors: Villafañe Jorge Hugo
Published: November 2023

This study constitutes a preview of a broader research project on kinship, family, and society in colonial La Rioja. In this context, the results obtained from the study of five generations of the Villafañe and Guzmán family are presented. Various aspects such as family organization, inheritance system, conflicts between families, the construction of a distinct identity, and the strategies that allowed this family to preserve its heritage and maintain a prominent status in the local elite are examined and analyzed in detail.
The Letter Cloth: Sensory Modes of the Epistolary in Prison Theatre Practice
Authors: McPhee Molly
Published: November 2023

In this article, I explore performances of letter writing within the archives of the London-based theatre company Clean Break, who work with justice-experienced women and women at risk. Clean Break’s archive at the Bishopsgate Institute in London contains an extensive collection of production ephemera and letters. Charting the company’s development across forty years of theatre productions, public advocacy, and work in prisons and community settings, these materials of the archive—strategic documents, annotated playscripts and rehearsal notes, production photography and correspondence—reveal the acute importance of the letter to people living on the immediate borderlands of the prison. Despite these generative resonances, however, the epistolary form is very rarely used in Clean Break’s theatre: as the archive reveals, since the company was founded by two women in HM Prison Askham Grange in 1979, stagings of letters have occurred in only a handful of instances. In this archival exploration of the epistolary in three works by Clean Break—a film broadcast by the BBC, a play staged at the Royal Court, and a circular chain-play written by women in three prisons—I investigate what lifeworlds beyond prison epistolary forms in performance propose.
An Attempt to Estimate the Social Value Resulting from Making Cultural Heritage Available in the Form of a Tourist Product
Authors: Szromek Adam R. , Bugdol Marek
Published: November 2023

Cultural heritage tourism is a multidimensional tourist activity because, apart from the advantages associated with practicing tourist activity, it is particularly associated with cognitive motives when it comes to practicing it, and even with discovering cultural identity. Such an activity has a specific value in relation to the adopted perspective. Researchers often attempt to determine the value that a tourism-related product has for the customer (tourist). Sometimes, it is defined as the value captured by the entity providing the tourism product. However, it is extremely rare for research to assess the social value of tourism. The aim of this work is to identify issues that can be used in measuring the social value resulting from cultural heritage made available in the form of a tourist product. The authors review the literature on cultural heritage and cultural identity, and then use a particular methodology to develop a tool for assessing social value. To develop their own research tool, the authors use the results of research conducted in 2021/2022 among managers of 70 cultural heritage tourism facilities in Europe. As a result, a tool was created that includes six statements that can contribute to the assessment of the social value resulting from the provision of material cultural heritage in the form of a tourist product. The reliability of the tool was estimated at 0.69 and its standardized rating at 0.71. The social value of cultural heritage, in the opinion of experts, achieved an average rating of 4.06 (on a scale from 1 to 5), with the 6-item version of the tool attaining an average rating of 4.10. Taking into account the usefulness of the proposed solutions, remember that the paper reflects only the European vision, which is one of many possible views.
The Roles of the PSEUDO-RESPONSE REGULATORs in Circadian Clock and Flowering Time in Medicago truncatula
Authors: Wang Xiao , Zhang Juanjuan , Liu Xiu , Kong Yiming , Han Lu
Published: November 2023

PSEUDO-RESPONSE REGULATORs (PRRs) play key roles in the circadian rhythms and flowering in plants. Here, we identified the four members of the PRR family in Medicago truncatula, including MtPRR9a, MtPRR9b, MtPRR7 and MtPRR5, and isolated their Tnt1 retrotransposon-tagged mutants. They were expressed in different organs and were nuclear-localized. The four MtPRRs genes played important roles in normal clock rhythmicity maintenance by negatively regulating the expression of MtGI and MtLHY. Surprisingly, the four MtPRRs functioned redundantly in regulating flowering time under long-day conditions, and the quadruple mutant flowered earlier. Moreover, MtPRR can recruit the MtTPL/MtTPR corepressors and the other MtPRRs to form heterodimers to constitute the core mechanism of the circadian oscillator.
DNA Damage, DNA Repair, and Cancer: Second Edition
Authors: Kuwahara Kazuhiko
Published: November 2023

Following our first Special Issue, we are pleased to present this Special Issue in the International Journal of Molecular Sciences, titled ‘DNA Damage, DNA Repair, and Cancer: Second Edition’ [...]
The Competing Endogenous RNAs Regulatory Genes Network Mediates Leaf Shape Variation and Main Effector Gene Function in Mulberry Plant (Morus alba)
Authors: Li Jianbin , Wang Lei , Ackah Michael , Amoako Frank Kwarteng , Jiang Zijie , Shi Yisu , Li Haonan , Zhao Weiguo
Published: November 2023

Mulberry plants (Morus alba) have leaf shapes, ranging from unlobed to lobed, which are crucial for yield, growth, and adaptability, indicating their ability to adapt to their environment. Competing endogenous RNAs (ceRNAs) constitute a web of RNAs within the organism’s transcriptional regulatory system, including protein-coding genes (mRNAs), microRNAs (miRNAs), long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and others. In this study, samples for ceRNA sequencing were categorized into two groups: whole leaves and lobed leaves, each group with three replicates. In addition, we isolated, cloned, and characterized the precursor miRNA (miR156x) from the leaves of M. alba. miR156x precursor had a length of 107 base pairs and a minimum folding free energy of 50.27 kcal/mol. We constructed a pCAMBIA-35S-GUS-miR156x dual overexpression vector and established a transient transformation system for mulberry. At an optimal transformation solution (OD600 = 0.7), the GUS gene showed a higher expression in the leaves of transiently transformed mulberry with miR156x overexpression, four days after transformation, while the target genes of miR156x had decreased expression in the same leaves. Investigations into the transgenic mulberry plants uncovered various modifications to physio-chemical parameters including POD, SOD, PRO, MDA, soluble proteins and sugars, and chlorophyl content. miRNAs in the plants were found to act as negative regulators of gene expression in response to changes in leaf shape regulation, which was confirmed in vitro using dual-luciferase reporter assays. Subsequently, we cloned Maspl3 in vitro and conducted GST-Pull down assays, obtaining multiple proteins that interacted with the Maspl3 gene. This indicates that the miR156x/Maspl3/MSTRG.25812.1 regulatory module contributes to the differences in mulberry leaf shape.
Editorial for the IJMS Special Issue on Sglt2 Inhibitors (Volume 2)
Authors: Lymperopoulos Anastasios
Published: November 2023

The goal of the second volume of this Special Issue was to build upon the success of the first one and to continue to highlight the ever-expanding list of pharmacological properties of the sodium/glucose co-transporter (SGLT) type 2 (SGLT2) inhibitor (SGLT2i) drug class (also known as gliflozins) [...]
Inhibitors of 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase Decrease the Growth, Ergosterol Synthesis and Generation of petite Mutants in Candida glabrata and Candida albicans
Authors: Andrade-Pavón Dulce , Sánchez-Sandoval Eugenia , Tamariz Joaquín , Ibarra Jose Antonio , Hernández-Rodríguez César , Villa-Tanaca Lourdes
Published: November 2023

Candida glabrata and Candida albicans, the most frequently isolated candidiasis species in the world, have developed mechanisms of resistance to treatment with azoles. Among the clinically used antifungal drugs are statins and other compounds that inhibit 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), resulting in decreased growth and ergosterol levels in yeasts. Ergosterol is a key element for the formation of the yeast cell membrane. However, statins often cause DNA damage to yeast cells, facilitating mutation and drug resistance. The aim of the current contribution was to synthesize seven series of compounds as inhibitors of the HMGR enzyme of Candida ssp., and to evaluate their effect on cellular growth, ergosterol synthesis and generation of petite mutants of C. glabrata and C. albicans. Compared to the reference drugs (fluconazole and simvastatin), some HMGR inhibitors caused lower growth and ergosterol synthesis in the yeast species and generated fewer petite mutants. Moreover, heterologous expression was achieved in Pichia pastoris, and compounds 1a, 1b, 6g and 7a inhibited the activity of recombinant CgHMGR and showed better binding energy values than for α-asarone and simvastatin. Thus, we believe these are good candidates for future antifungal drug development.
Major Depressive Disorder and Gut Microbiota: Role of Physical Exercise
Authors: Souza Pedro Borges de , de Araujo Borba Laura , Castro de Jesus Louise , Valverde Ana Paula , Gil-Mohapel Joana , Rodrigues Ana Lúcia S.
Published: November 2023

Major depressive disorder (MDD) has a high prevalence and is a major contributor to the global burden of disease. This psychiatric disorder results from a complex interaction between environmental and genetic factors. In recent years, the role of the gut microbiota in brain health has received particular attention, and compelling evidence has shown that patients suffering from depression have gut dysbiosis. Several studies have reported that gut dysbiosis-induced inflammation may cause and/or contribute to the development of depression through dysregulation of the gut–brain axis. Indeed, as a consequence of gut dysbiosis, neuroinflammatory alterations caused by microglial activation together with impairments in neuroplasticity may contribute to the development of depressive symptoms. The modulation of the gut microbiota has been recognized as a potential therapeutic strategy for the management of MMD. In this regard, physical exercise has been shown to positively change microbiota composition and diversity, and this can underlie, at least in part, its antidepressant effects. Given this, the present review will explore the relationship between physical exercise, gut microbiota and depression, with an emphasis on the potential of physical exercise as a non-invasive strategy for modulating the gut microbiota and, through this, regulating the gut–brain axis and alleviating MDD-related symptoms.
Dopaminergic Projections from the Hypothalamic A11 Nucleus to the Spinal Trigeminal Nucleus Are Involved in Bidirectional Migraine Modulation
Authors: Li Chenhao , Li Yang , Zhang Wenwen , Ma Zhenjie , Xiao Shaobo , Xie Wei , Miao Shuai , Li Bozhi , Lu Guangshuang , Liu Yingyuan , Bai Wenhao , Yu Shengyuan
Published: November 2023

Clinical imaging studies have revealed that the hypothalamus is activated in migraine patients prior to the onset of and during headache and have also shown that the hypothalamus has increased functional connectivity with the spinal trigeminal nucleus. The dopaminergic system of the hypothalamus plays an important role, and the dopamine-rich A11 nucleus may play an important role in migraine pathogenesis. We used intraperitoneal injections of glyceryl trinitrate to establish a model of acute migraine attack and chronicity in mice, which was verified by photophobia experiments and von Frey experiments. We explored the A11 nucleus and its downstream pathway using immunohistochemical staining and neuronal tracing techniques. During acute migraine attack and chronification, c-fos expression in GABAergic neurons in the A11 nucleus was significantly increased, and inhibition of DA neurons was achieved by binding to GABA A-type receptors on the surface of dopaminergic neurons in the A11 nucleus. However, the expression of tyrosine hydroxylase and glutamic acid decarboxylase proteins in the A11 nucleus of the hypothalamus did not change significantly. Specific destruction of dopaminergic neurons in the A11 nucleus of mice resulted in severe nociceptive sensitization and photophobic behavior. The expression levels of the D1 dopamine receptor and D2 dopamine receptor in the caudal part of the spinal trigeminal nucleus candalis of the chronic migraine model were increased. Skin nociceptive sensitization of mice was slowed by activation of the D2 dopamine receptor in SP5C, and activation of the D1 dopamine receptor reversed this behavioral change. GABAergic neurons in the A11 nucleus were activated and exerted postsynaptic inhibitory effects, which led to a decrease in the amount of DA secreted by the A11 nucleus in the spinal trigeminal nucleus candalis. The reduced DA bound preferentially to the D2 dopamine receptor, thus exerting a defensive effect against headache.
Inhibition of Spontaneous Hepatocarcinogenesis by 4,5-Didehydrogeranylgeranoic Acid: Effects of Small-Dose and Infrequent Administration
Authors: Omori Masahide , Shidoji Yoshihiro , Moriwaki Hisataka
Published: November 2023

Inhibitory effects of 4,5-didehydrogeranylgeranoic acid (dGGA) on the development of tumors were investigated in spontaneous hepatoma mice, C3H/HeNCrj. Experiment 1: Male mice at 8 weeks of age were raised on a basal diet, and then provided with a diet containing 0.02% dGGA from 32 to 91 weeks of age. Experiment 2: dGGA was administered to the animals only once at different time points, from 2 months to 17 months after birth, respectively. Experiment 3: dGGA was administered twice to the mice at different time points: at 5 months and 11 months, and at 8 months and 11 months, respectively. When the inhibitory effects on tumor development were evaluated with the incidences of tumors, average numbers, and weight of tumors per mouse, there was a marked relationship between the time of single or dual dosing and the inhibitory effects of dGGA. The greatest inhibitory effects were observed in Experiment 3 in the group of animals given dGGA at the ages of 8 and 11 months, which were far superior to the results with a large dose of the compounds for a long time. These results might indicate that dGGA administered at the right time in the right amount effectively prevents the development of cancer.
AdvRain: Adversarial Raindrops to Attack Camera-Based Smart Vision Systems
Authors: Guesmi Amira , Hanif Muhammad Abdullah , Shafique Muhammad
Published: November 2023

Vision-based perception modules are increasingly deployed in many applications, especially autonomous vehicles and intelligent robots. These modules are being used to acquire information about the surroundings and identify obstacles. Hence, accurate detection and classification are essential to reach appropriate decisions and take appropriate and safe actions at all times. Current studies have demonstrated that “printed adversarial attacks”, known as physical adversarial attacks, can successfully mislead perception models such as object detectors and image classifiers. However, most of these physical attacks are based on noticeable and eye-catching patterns for generated perturbations making them identifiable/detectable by the human eye, in-field tests, or in test drives. In this paper, we propose a camera-based inconspicuous adversarial attack (AdvRain) capable of fooling camera-based perception systems over all objects of the same class. Unlike mask-based FakeWeather attacks that require access to the underlying computing hardware or image memory, our attack is based on emulating the effects of a natural weather condition (i.e., Raindrops) that can be printed on a translucent sticker, which is externally placed over the lens of a camera whenever an adversary plans to trigger an attack. Note, such perturbations are still inconspicuous in real-world deployments and their presence goes unnoticed due to their association with a natural phenomenon. To accomplish this, we develop an iterative process based on performing a random search aiming to identify critical positions to make sure that the performed transformation is adversarial for a target classifier. Our transformation is based on blurring predefined parts of the captured image corresponding to the areas covered by the raindrop. We achieve a drop in average model accuracy of more than 45% and 40% on VGG19 for ImageNet dataset and Resnet34 for Caltech-101 dataset, respectively, using only 20 raindrops.
Could the Combination of eGFR and mGPS Facilitate the Differential Diagnosis of Age-Related Renal Decline from Diseases? A Large Study on the Population of Western Sicily
Authors: Carella Miriam , Porreca Annamaria , Piazza Cinzia , Gervasi Francesco , Magro Daniele , Venezia Marika , Verso Raffaella Lo , Vitale Giuseppe , Agnello Annalisa Giusy , Scola Letizia , Aronica Tommaso Silvano , Balistreri Carmela Rita
Published: November 2023

The assessment of renal function is critical to diagnosing and managing renal age-related decline, disease (KD), and failure, which are prevalent in the elderly population. The glomerular filtration rate (GFR) is widely used as an indicator of kidney function, but its direct measurement is challenging, as are its age and gender caveats. This makes difficult the differential diagnosis between age-related physiological decline and KD and/or failure. Currently, the inflammation-based modified Glasgow prognostic score (mGPS) is emerging as a promising biomarker of several inflammatory acute/chronic diseases. In this study, the large variability of eGFR with age and gender was evaluated as the association of eGFR values with mGPS levels. A population of 57,449 adult participants (age ≥ 18 years) was enrolled. Appropriate circulating biomarkers were measured to detect eGFR and mGPS values. The data obtained demonstrated a significant decrease in eGFR in men vs. women across the four selected age classes (18–40, 40–60, 60–80, 80–100 years); eGFR classes were significantly associated with mGPS (p < 0.001), as were age classes and gender with mGPS categories. Accordingly, the percentage of people having an mGPS score = 2 significantly increased across the eGFR classes: with an 11% in the G1/eGFR class needed to achieve 44% in G5/eGFR. Thus, the combination of mGPS with eGFR could represent the best benchmark risk model for the differential diagnosis of kidney disease from the age-related eGFR reduction.
Prognostic Value of the Baseline and Early Changes in Monocyte-to-Lymphocyte Ratio for Short-Term Mortality among Critically Ill Patients with Acute Kidney Injury
Authors: Luo Xinyao , Wan Dingyuan , Xia Ruoxin , Liao Ruoxi , Su Baihai
Published: November 2023

(1) Background: Inflammation plays an important role in the onset and progression of acute kidney injury (AKI). Despite this, evidence regarding the prognostic effect of the monocyte-to-lymphocyte ratio (MLR), a novel systemic inflammation marker, among patients with AKI is scarce. This study sets out to investigate the prognostic potential of both baseline and early changes in MLR for short-term mortality among critically ill patients with AKI. (2) Method: Eligible patients with AKI from the Medical Information Mart for Intensive Care IV database were retrospectively analyzed. MLR cutoff values were determined using maximally selected rank statistics and tertiles. The clinical outcomes were 30-day and 90-day mortality in the intensive care unit. A restricted cubic splines model and Cox proportional hazards models were utilized to evaluate the association between the baseline MLR and short-term mortality. Then, the trends in MLR over time were compared between the 30-day survivors and non-survivors using a generalized additive mixed model (GAMM). (3) Result: A total of 15,986 patients were enrolled. Multivariable Cox regression analysis identified baseline MLR ≥ 0.48 as an independent risk factor predicting 30-day mortality (HR 1.33, 95%CI 1.24, 1.45, p < 0.001) and 90-day mortality (HR 1.34, 95%CI 1.23, 1.52, p < 0.001) after adjusting for potential confounders. Similar trends were observed for 30-day and 90-day mortality when tertiles were used to group patients. The restricted cubic splines model revealed a non-linear association between MLR and 30-day and 90-day mortality (both p for non-linear < 0.001, both p for overall < 0.001). The area under the curve of 0.64 for MLR was higher than that of monocytes (0.55) and lymphocytes (0.61). In the subgroup analyses, despite the noted significant interactions, the direction of the observed association between MLR and 30-day mortality was consistent across most prespecified subgroups, except for shock and black ethnicity. The GAMM results highlighted that, as time went on, MLR in the 30-day survival group consistently declined, whereas MLR in the non-survival group rose within 15 days post-ICU admission. The difference between the two groups persisted significantly even after adjusting for confounders (p = 0.006). (4) Conclusion: A higher baseline MLR was identified as an independent risk factor predicting 30-day and 90-day mortality. The early increase in MLR was associated with high 30-day mortality, suggesting that dynamic monitoring of MLR could potentially better predict survival in critically ill patients with AKI.
Upregulation of Platelet-Activating Factor Receptor Expression and Lyso-Platelet-Activating Factor Isoforms in Human Nasal Polyp Tissues
Authors: Roca-Ferrer Jordi , Pérez-González Maria , Alobid Isam , Tubita Valeria , Fuentes Mireya , Bantulà Marina , Muñoz-Cano Rosa , Valero Antonio , Izquierdo Iñaki , Mullol Joaquim
Published: November 2023

Background: The Platelet-Activating Factor (PAF)/receptor (PAFR) system is involved in asthma and allergic rhinitis; however, its role in chronic rhinosinusitis (CRS) is still unclear. This study aimed to assess the expression of PAFR and the concentration of Lyso-PAF isoforms in the nasal polyps (NP) of patients suffering from CRS with/without comorbidities such as asthma and NSAID-exacerbated respiratory disease (N-ERD) compared to healthy nasal mucosa (NM) from control subjects. Methods: NM (n = 8) and NP tissues were obtained from patients undergoing surgery for septal deviation/turbinate hypertrophy or endoscopic sinus surgery, respectively. Three phenotypes were studied: CRSwNP with no asthma (n = 6), CRSwNP with non-steroidal anti-inflammatory drug (NSAID)-tolerant asthma (n = 6), and CRSwNP with NSAID-exacerbated respiratory disease (n = 6). PAFR protein and mRNA were assessed via immunochemistry, immunofluorescence, Western blot, and real-time quantitative PCR. Lyso-PAF isoforms (C16, C18, and C18:1) were quantified via mass spectrometry. Results: PAFR protein was expressed in the NM and NP, concretely in epithelial cells and submucosal glands. Compared to NM, PAFR mRNA expression was higher in all NP phenotypes (p < 0.05) while all Lyso-PAF isoform concentrations were higher in the NP from asthmatic patients (p < 0.05). Lyso-PAF C16 and C18 concentrations were higher in the NP from asthmatic patients than in the NP from patients without asthma. Conclusions: The PAF/PAFR system could play a pathophysiological role in CRSwNP pathogenesis.
Risk of Preterm Birth among Secundiparas with a Previous Cesarean due to a Failed Vacuum Delivery
Authors: Helman Sarit , Mahajna Muhammad , Ehrlich Zvi , Ratner Miri , Grisaru-Granovsky Sorina , Reichman Orna
Published: November 2023

Background: Studies have found an association between second-stage cesarean sections (SSCSs) and subsequent preterm birth (PTB). We aimed to evaluate if secundiparas with previous second-stage cesarean sections due to a failed vacuum delivery (SSCS-F-VD) are associated with PTB in the subsequent delivery compared with secundiparas with previous spontaneous vaginal birth (SVB) at term. A secondary aim was to compare this association with secundiparas with a previous SSCS at term. Methods: A historical, prospective, longitudinal cohort study was conducted in a large tertiary university hospital between 2006 and 2019. Matched mothers who experienced first and second births at the indexed hospital, excluding those with a previous miscarriage or multiple pregnancy in either the first or second birth were grouped based on the mode of delivery and gestational week of the first birth. Results: Parturients with term SVB and term SSCSs were less likely to experience PTB in the following delivery compared with those who underwent an SSCS-F-VD, with 496/14,551 (3.4%) versus 6/160 (3.8%) versus 5/61 (8.2%), respectively, at p < 0.001. A logistic regression model revealed that secundiparas with previous SSCS-F-VD had an association with PTB in the following delivery compared with term SVB, with an OR of 2.756 (1.097; 6.922, p = 0.031). Conclusion: Previous SSCS-F-VD is associated with PTB in the following delivery, offering valuable insights for pregnancy management and patient counseling.
Restoration of Over-Ground Walking via Non-Invasive Neuromodulation Therapy: A Single-Case Study
Authors: Alam Monzurul , Ling Yan To , Rahman Md Akhlasur , Wong Arnold Yu Lok , Zhong Hui , Edgerton V. Reggie , Zheng Yong-Ping
Published: November 2023

Spinal cord injuries (SCI) can result in sensory and motor dysfunctions, which were long considered permanent. Recent advancement in electrical neuromodulation has been proven to restore sensorimotor function in people with SCI. These stimulation protocols, however, were mostly invasive, expensive, and difficult to implement. In this study, transcutaneous electrical stimulation (tES) was used to restore over-ground walking of an individual with 21 years of chronic paralysis from a cervical SCI. After a total of 66 weeks of rehabilitation training with tES, which included standing, functional reaching, reclined sit-up, treadmill walking, and active biking, significant improvement in lower-limb volitional movements and overall light touch sensation were shown as measured by the International Standards for Neurological Classification of Spinal Cord Injury (ISNCSCI) score. By the end of the study, the participant could walk in a 4-m walking test with the aid of a walking frame and ankle–foot orthoses. The successful sensorimotor recovery of our study participant sheds light on the future of non-invasive neuromodulation treatment for SCI paralysis.
Assessing the Effects of 0.3% Carboxymethylcellulose Tear Substitute Treatment on Symptoms and Signs of Dry Eye Disease in Elderly Population: A Prospective Longitudinal Study
Authors: Ballesteros-Sánchez Antonio , Sánchez-González José-María , Tedesco Giovanni Roberto , Rocha-De-Lossada Carlos , Murano Gianluca , Spinelli Antonio , Borroni Davide
Published: November 2023

Background: We aimed to evaluate the effects of 0.3% carboxymethylcellulose (CMC) tear substitute treatment in dry eye disease (DED), as well as treatment compliance and adverse events (AEs). Methods: In this prospective, longitudinal study, a total of 30 eyes receiving 0.3% CMC tear substitute four times daily for DED were evaluated. Clinical endpoints included an ocular surface disease index (OSDI) questionnaire, average non-invasive tear film break-up time (A-NIBUT), lipid layer thickness (LLT), and a Schirmer test with anesthesia (ST). Treatment compliance and AEs were also assessed. All evaluations were performed at 2, 4, and 12 weeks of follow-up. Results: At the end of the follow-up, significant improvement was observed in all clinical endpoints with the following mean values: ΔOSDI questionnaire of −22.53 ± 14.68 points, ΔA-NIBUT of 4.81 ± 2.88 s, ΔLLT of 5.63 ± 6.53 nm, and ΔST of 2.8 ± 2.1 mm (p < 0.001 for all comparisons). Although repeated measures analysis showed that all clinical endpoints presented statistically significant differences (p < 0.001 for all comparisons LLTBaseline–LLT2-weeks (p = 0.460) and LLT4-weeks–LLT12-weeks (p = 0.071) were the only pairs of measures that reported non-statistically significant differences). In addition, treatment compliance was 94.3 ± 5.2% and transient AEs related to the use of 0.3% CMC tear substitute were reported. Conclusions: 0.3% CMC tear substitute treatment seems to achieve beneficial effects on the OSDI questionnaire, A-NIBUT, LLT, and ST. However, further studies at this concentration are needed to confirm these results.
The Compensation Index Is Better Associated with DSA ASITN Collateral Score Compared to the Cerebral Blood Volume Index and Hypoperfusion Intensity Ratio
Authors: Lakhani Dhairya A. , Balar Aneri B. , Koneru Manisha , Wen Sijin , Hoseinyazdi Meisam , Greene Cynthia , Xu Risheng , Luna Licia , Caplan Justin , Dmytriw Adam A. , Guenego Adrien , Wintermark Max , Gonzalez Fernando , Urrutia Victor , Huang Judy , Nael Kambiz , Rai Ansaar T. , Albers Gregory W. , Heit Jeremy J. , Yedavalli Vivek S.
Published: November 2023

Background: Pretreatment CT Perfusion (CTP) parameters serve as reliable surrogates of collateral status (CS). In this study, we aim to assess the relationship between the novel compensation index (CI, Tmax > 4 s/Tmax > 6 s) and already established CTP collateral markers, namely cerebral blood volume (CBV) index and Hypoperfusion Intensity Ratio (HIR), with the reference standard American Society of Interventional and Therapeutic Neuroradiology (ASITN) collateral score (CS) on DSA. Methods: In this retrospective study, inclusion criteria were the following: (a) CT angiography confirmed anterior circulation large vessel occlusion from 9 January 2017 to 10 January 2023; (b) diagnostic CT perfusion; and (c) underwent mechanical thrombectomy with documented DSA-CS. Student t-test, Mann–Whitney-U-test and Chi-square test were used to assess differences. Spearman’s rank correlation and logistic regression analysis were used to assess associations. p ≤ 0.05 was considered significant. Results: In total, 223 patients (mean age: 67.8 ± 15.8, 56% female) met our inclusion criteria. The CI (ρ = 0.37, p < 0.001) and HIR (ρ = −0.29, p < 0.001) significantly correlated with DSA-CS. Whereas the CBV Index (ρ = 0.1, p > 0.05) did not correlate with DSA-CS. On multivariate logistic regression analysis taking into account age, sex, ASPECTS, tPA, premorbid mRS, NIH stroke scale, prior history of TIA, stroke, atrial fibrillation, diabetes mellitus, hyperlipidemia, heart disease and hypertension, only CI was not found to be independently associated with DSA-CS (adjusted OR = 1.387, 95% CI: 1.09–1.77, p < 0.01). Conclusion: CI demonstrates a stronger correlation with DSA-CS compared to both the HIR and CBV Index where it may show promise as an additional quantitative pretreatment CS biomarker.
Comparative Evaluation of Visual Performance and Patient Satisfaction following Cataract Surgery: A Retrospective Analysis of an Extended Depth-of-Focus Intraocular Lens and a Diffractive Multifocal Lens with Extended Depth of Focus
Authors: Han Kwang Eon , Lee Ji Eun
Published: November 2023

(1) Background: Cataract surgery has evolved significantly with the development of multifocal and extended depth-of-focus intraocular lenses (IOLs), driven by increasing patient desire for spectacle independence. (2) Methods: This retrospective, single-center study conducted on 86 eyes from 59 patients aged 40–80 years compared the performance and patient satisfaction of Symfony® and Synergy® (Johnson & Johnson Vision) IOLs during a follow-up of 1 year postsurgery. Uncorrected and corrected distance, intermediate, and near visual acuities were assessed (UDVA, CDVA, UIVA, CIVA, UNVA, and CNVA, respectively). (3) Results: Although both IOLs demonstrated a commendable distance VA, Synergy® outperformed in near VA (UNVA, p = 0.040; CNVA, p = 0.014), and Symfony® slightly excelled in intermediate VA (UIVA, p = 0.014; CIVA, p = 0.040). The defocus curve of Synergy® maintained a higher VA even at −4 D with a smoother curve and a broad landing zone. Although the optical quality assessments were similar, Symfony® had a nonsignificant edge. Patients indicated higher satisfaction and reduced reliance on spectacles with Synergy® despite more frequent reports of glare and halos. (4) Conclusions: These findings highlight the importance of personalized IOL selection in cataract surgery, which must be customized to apply the distinctive advantages of each IOL to address the unique visual requirements and lifestyle of patients.