bluuebunny/crossref_metadata_2025 huggingface dataset:
Relationship between Mild Cognitive Impairment, Pre-Frailty, Physical and Psychological Functioning, and Functional Capacity among Community-Dwelling Older Adults
Authors: Katsurasako Tsuyoshi , Murata Shin , Goda Akio , Shiraiwa Kayoko , Horie Jun , Abiko Teppei , Nakano Hideki
Published: September 2023

Mild cognitive impairment (MCI) is associated with a high risk of dementia. Reportedly, 54.6% of older adults with MCI fall into the pre-frailty category. However, it is unclear what differences exist in older adults with regard to their physical, psychological, and functional capacities, which depend on MCI, pre-frailty, or a combination of the two. This study aimed to examine the differences between the association between physical function, psychological function, and functional capacity by examining a combination of MCI and pre-frailty among community-dwelling older Japanese individuals. The participants in the analysis were 236 older people living in the community. They were classified into four groups, as follows: normal, MCI only, pre-frailty only, and pre-frailty/MCI; furthermore, their physical, psychological, and functional capacities were compared. In addition, a multinomial logistic regression analysis was performed using MCI and pre-frailty as dependent variables. No associated factors were identified for MCI only, and a depressive mood was only associated with pre-frailty. When pre-frailty and MCI were combined, knee extension muscle strength, fastest gait speed, the s30 s chair stand test, depressed moods, and the instrumental activities of daily living (IADL) were correlated. Our results suggest that when MCI and pre-frailty are combined, declines in both physical and psychological functions and IADL are likely to occur.
In Vitro Propagation of Peumus boldus Mol, a Woody Medicinal Plant Endemic to the Sclerophyllous Forest of Central Chile
Authors: Guerra Francesca , Badilla Loreto , Cautín Ricardo , Castro Mónica
Published: September 2023

Peumus boldus Mol (P. boldus), a Chilean endemic tree species occurring in sclerophyllous or evergreen forests, has historically played a significant role in indigenous ancestral medicine. Recently, P. boldus has garnered renewed attention, spurred by the growing interest in its leaves and bark. These parts contain a wealth of bioactive agents, including alkaloids, flavonoids, essential oils, and potent antioxidant properties attributed to their high phenolic compound content. However, the species’ regeneration within its native habitat has been hindered by a confluence of factors such as climate change, agricultural activities, and shifts in land use. Coupled with the seeds’ low germination rate and protracted emergence period, the necessity to develop large-scale propagation methods to bolster P. boldus population numbers has become increasingly evident. Furthermore, the widespread use of P. boldus for medicinal purposes renders it vulnerable to overexploitation, thereby underscoring the need for a comprehensive mass propagation protocol to support conservation efforts. Thus, the main objective of this study was to formulate an in vitro protocol for mass regeneration of P. boldus. The explants excised from nodal sections demonstrated an average survival rate of 74%, while the application of 6-benzylaminopurine (4.44 μM) yielded an average shoot length of 5.9 cm. In vitro shoot rooting achieved a success rate of 80% using perlite supplemented with indole-3-butyric acid (9.84 μM). During the acclimation phase, in vitro rooted plants displayed a remarkable 100% survival rate at the 30-day mark after being transplanted into a substrate consisting of perlite and peat in a (1:1 v/v) ratio.
Edible Composite Coating of Chitosan and Curdlan Maintains Fruit Quality of Postharvest Cherry Tomatoes
Authors: Yu Youwei , Yan Kejing , Zhang Huanhuan , Song Yanyin , Chang Yuan , Liu Kunyu , Zhang Shaoying , Cui Meilin
Published: September 2023

Postharvest cherry tomatoes are prone to senescence, decay and nutrient loss during the storage period owing to microbial invasion and their own metabolism. In this work, postharvest cherry tomatoes were treated with a composite coating of 1% chitosan and 1% curdlan, and the characteristics of postharvest cherry tomatoes during storage were investigated. Compared to control samples, after 21 d of storage under ambient conditions, the cherry tomatoes treated with the chitosan and curdlan coatings showed less rottenness, less weight loss, a lower respiration rate, reduced ethylene production, lower malonaldehyde (MDA) content and reduced membrane permeability. After the samples were treated with the composite coating, the activities of free radical scavenging enzymes such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were maintained at higher levels; the activities of related disease-resistant enzymes such as chitinase (CHI) and glucanase (GLU) were also maintained at higher levels. The soluble solids, titratable acidity, firmness, vitamin C content, lycopene content and antioxidant activities of postharvest cherry tomatoes treated with the composite coating exhibited higher levels as well. The composite coating of chitosan and curdlan might be a potentially promising method for preserving postharvest cherry tomatoes and other fruits.
Tomato Leaf Disease Recognition via Optimizing Deep Learning Methods Considering Global Pixel Value Distribution
Authors: Li Zheng , Tao Weijie , Liu Jianlei , Zhu Fenghua , Du Guangyue , Ji Guanggang
Published: September 2023

In image classification of tomato leaf diseases based on deep learning, models often focus on features such as edges, stems, backgrounds, and shadows of the experimental samples, while ignoring the features of the disease area, resulting in weak generalization ability. In this study, a self-attention mechanism called GD-Attention is proposed, which considers global pixel value distribution information and guide the deep learning model to give more concern on the leaf disease area. Based on data augmentation, the proposed method inputs both the image and its pixel value distribution information to the model. The GD-Attention mechanism guides the model to extract features related to pixel value distribution information, thereby increasing attention towards the disease area. The model is trained and tested on the Plant Village (PV) dataset, and by analyzing the generated attention heatmaps, it is observed that the disease area obtains greater weight. The results achieve an accuracy of 99.97% and 27 MB parameters only. Compared to classical and state-of-the-art models, our model showcases competitive performance. As a next step, we are committed to further research and application, aiming to address real-world, complex scenarios.
Potential Impacts of Climate Change on the Al Abila Dam in the Western Desert of Iraq
Authors: Abed Rasha , Adham Ammar , Allawi Mohammed Falah , Ritsema Coen
Published: September 2023

The potential impacts resulting from climate change will cause significant global problems, particularly in underdeveloped nations where the effects are felt the most. Techniques for harvesting water such as small dams provide an alternative supply of water and are adaptive solutions to deal with water scarcity in the context of future climate change. However, it is difficult to determine how rainwater harvesting (dams) may be impacted by climate change since general circulation models (GCMs), widely utilized for predicting potential future climate change scenarios, work on an extremely large scale. The primary aim of this research was to quantify the effect of climate change on water availability at the catchment scale by statistically downscaling temperature and rainfall from the GCMs. Then, using a water harvesting model, the performance of the Abila Dam in Iraq’s western desert was evaluated in both the current climate (1990–2020) and various future climate change scenarios (2020–2100). Precipitation generally decreases as the annual temperature increases. To simulate future water availability, these changes in meteorological factors were incorporated into the water harvesting model. In total, 15% or less of net storage might fulfil the whole storage capacity during the baseline period, whereas it is 10% in RCP 2.6 in 2011–2040 for future scenarios. In contrast, RCP 8.5 will be able to meet water needs at a pace of 6% in 2011–2040. The findings of this study proved that the Al Abila dam will be unable to supply the necessary water for the area surrounding the Al Abila dam in the future scenarios.
Psychometric Properties of the Greek Version of the Connor–Davidson Resilience Scale (CD-RISC-10) in a Sample of Nurses
Authors: Galanis Petros , Psomiadi Maria Elissavet , Karagkounis Chrysovalantis , Liamopoulou Polyxeni , Manomenidis Georgios , Panayiotou Georgios , Bellali Thalia
Published: September 2023

Resilience has been defined as one’s ability to maintain a mental health state and overall well-being when undergoing grave stress or facing significant adversity. Numerous resilience-investigating research tools have been developed over the years, with the Connor–Davidson Resilience Scale (CD-RISC), a self-rated tool presenting valuable psychometric properties, remaining one of the most prominent. We aimed to translate and validate the brief CD-RISC-10 in a convenience sample of 584 nurses in Greece’s secondary and tertiary health care system. We conducted a confirmatory factor analysis and known-groups validity test and estimated the reliability of the CD-RISC-10. Our confirmatory factor analysis revealed that the scale had a unifactorial structure since all the model fit indices were very good. Moreover, the reliability of the CD-RISC-10 was very good since the acquired Cronbach’s alpha and McDonald’s omega were 0.924 and 0.925, respectively. Therefore, the Greek version of the CD-RISC-10 confirmed the factor structure of the original scale and had very good validity and reliability.
Evaluation of Different Natural Ventilation Strategies by Monitoring the Indoor Air Quality Using CO2 Sensors
Authors: Sánchez-Fernández Ana , Coll-Aliaga Eloína , Lerma-Arce Victoria , Lorenzo-Sáez Edgar
Published: September 2023

Indoor air quality is a characteristic that depends on air pollutants inside a building and that can be affected by different ventilation strategies. There is strong evidence linking poor indoor air quality (IAQ) and harmful health effects, especially on vulnerable collectives, such as children in schools. Due to this concern, this work aims to provide guidance on the design of highly efficient ventilation strategies to improve the air quality of schools’ classrooms. For this, IAQ monitoring has been carried out in eight educational in real conditions centres using CO2 concentration as an IAQ indicator. Variables such as the presence of students and their number, activity developed in the classroom and ventilation strategy used together with break time duration have been also recorded to analyse their influence on CO2 concentration levels. Concluding results have allowed us to determine the maximum number of students allowed in a closed room to maintain CO2 levels at normal concentrations and the time needed to reduce these CO2 levels depending on the ventilation strategy adopted. Moreover, it has been discussed how surrounding school conditions (pollution or noise) and the building isolation are impacting the final IAQ in the classrooms studied.
MicroRNAs Present in Malignant Pleural Fluid Increase the Migration of Normal Mesothelial Cells In Vitro and May Help Discriminate between Benign and Malignant Effusions
Authors: Marqués Marta , Pont Mariona , Hidalgo Iván , Sorolla Maria Alba , Parisi Eva , Salud Antonieta , Sorolla Anabel , Porcel José M.
Published: September 2023

The sensitivity of pleural fluid (PF) analyses for the diagnosis of malignant pleural effusions (MPEs) is low to moderate. Knowledge about the pathobiology and molecular characteristics of this condition is limited. In this study, the crosstalk between stromal cells and tumor cells was investigated in vitro in order to reveal factors that are present in PF which can mediate MPE formation and aid in discriminating between benign and malignant etiologies. Eighteen PF samples, in different proportions, were exposed in vitro to mesothelial MeT-5A cells to determine the biological effects on these cells. Treatment of normal mesothelial MeT-5A cells with malignant PF increased cell viability, proliferation, and migration, and activated different survival-related signaling pathways. We identified differentially expressed miRNAs in PF samples that could be responsible for these changes. Consistently, bioinformatics analysis revealed an enrichment of the discovered miRNAs in migration-related processes. Notably, the abundance of three miRNAs (miR-141-3p, miR-203a-3, and miR-200c-3p) correctly classified MPEs with false-negative cytological examination results, indicating the potential of these molecules for improving diagnosis. Malignant PF produces phenotypic and functional changes in normal mesothelial cells. These changes are partly mediated by certain miRNAs, which, in turn, could serve to differentiate malignant from benign effusions.
Genomic and Transcriptomic Insights into the Evolution and Divergence of MIKC-Type MADS-Box Genes in Carica papaya
Authors: Dai Yunsu , Wang Yu , Zeng Liwang , Jia Ruizong , He Linwen , Huang Xueying , Zhao Hui , Liu Difa , Zhao Haixu , Hu Shuai , Gao Ling , Guo Anping , Xia Wei , Ji Changmian
Published: September 2023

MIKC-type MADS-box genes, also known as type II genes, play a crucial role in regulating the formation of floral organs and reproductive development in plants. However, the genome-wide identification and characterization of type II genes as well as a transcriptomic survey of their potential roles in Carica papaya remain unresolved. Here, we identified and characterized 24 type II genes in the C. papaya genome, and investigated their evolutional scenario and potential roles with a widespread expression profile. The type II genes were divided into thirteen subclades, and gene loss events likely occurred in papaya, as evidenced by the contracted member size of most subclades. Gene duplication mainly contributed to MIKC-type gene formation in papaya, and the duplicated gene pairs displayed prevalent expression divergence, implying the evolutionary significance of gene duplication in shaping the diversity of type II genes in papaya. A large-scale transcriptome analysis of 152 samples indicated that different subclasses of these genes showed distinct expression patterns in various tissues, biotic stress response, and abiotic stress response, reflecting their divergent functions. The hub-network of male and female flowers and qRT-PCR suggested that TT16-3 and AGL8 participated in male flower development and seed germination. Overall, this study provides valuable insights into the evolution and functions of MIKC-type genes in C. papaya.
The Role of SNAP-25 in Autism Spectrum Disorders Onset Patterns
Authors: Bolognesi Elisabetta , Guerini Franca Rosa , Carta Alessandra , Chiappedi Matteo , Sotgiu Stefano , Mensi Martina Maria , Agliardi Cristina , Zanzottera Milena , Clerici Mario
Published: September 2023

Autism spectrum disorders (ASD) can present with different onset and timing of symptom development; children may manifest symptoms early in their first year of life, i.e., early onset (EO-ASD), or may lose already achieved skills during their second year of life, thus showing a regressive-type onset (RO-ASD). It is still controversial whether regression represents a neurobiological subtype of ASD, resulting from distinct genetic and environmental causes. We focused this study on the 25 kD synaptosomal-associated protein (SNAP-25) gene involved in both post-synaptic formation and adhesion and considered a key player in the pathogenesis of ASD. To this end, four single nucleotide polymorphisms (SNPs) of the SNAP-25 gene, rs363050, rs363039, rs363043, and rs1051312, already known to be involved in neurodevelopmental and psychiatric disorders, were analyzed in a cohort of 69 children with EO-ASD and 58 children with RO-ASD. Both the rs363039 G allele and GG genotype were significantly more frequently carried by patients with EO-ASD than those with RO-ASD and healthy controls (HC). On the contrary, the rs1051312 T allele and TT genotype were more frequent in individuals with RO-ASD than those with EO-ASD and HC. Thus, two different SNAP-25 alleles/genotypes seem to discriminate between EO-ASD and RO-ASD. Notably, rs1051312 is located in the 3′ untranslated region (UTR) of the gene and is the target of microRNA (miRNA) regulation, suggesting a possible epigenetic role in the onset of regressive autism. These SNPs, by discriminating two different onset patterns, may represent diagnostic biomarkers of ASD and may provide insight into the different biological mechanisms towards the development of better tailored therapeutic and rehabilitative approaches.
Treatment of Status Epilepticus after Traumatic Brain Injury Using an Antiseizure Drug Combined with a Tissue Recovery Enhancer Revealed by Systems Biology
Authors: Kajevu Natallie , Lipponen Anssi , Andrade Pedro , Bañuelos Ivette , Puhakka Noora , Hämäläinen Elina , Natunen Teemu , Hiltunen Mikko , Pitkänen Asla
Published: September 2023

We tested a hypothesis that in silico-discovered compounds targeting traumatic brain injury (TBI)-induced transcriptomics dysregulations will mitigate TBI-induced molecular pathology and augment the effect of co-administered antiseizure treatment, thereby alleviating functional impairment. In silico bioinformatic analysis revealed five compounds substantially affecting TBI-induced transcriptomics regulation, including calpain inhibitor, chlorpromazine, geldanamycin, tranylcypromine, and trichostatin A (TSA). In vitro exposure of neuronal-BV2-microglial co-cultures to compounds revealed that TSA had the best overall neuroprotective, antioxidative, and anti-inflammatory effects. In vivo assessment in a rat TBI model revealed that TSA as a monotherapy (1 mg/kg/d) or in combination with the antiseizure drug levetiracetam (LEV 150 mg/kg/d) mildly mitigated the increase in plasma levels of the neurofilament subunit pNF-H and cortical lesion area. The percentage of rats with seizures during 0–72 h post-injury was reduced in the following order: TBI-vehicle 80%, TBI-TSA (1 mg/kg) 86%, TBI-LEV (54 mg/kg) 50%, TBI-LEV (150 mg/kg) 40% (p < 0.05 vs. TBI-vehicle), and TBI-LEV (150 mg/kg) combined with TSA (1 mg/kg) 30% (p < 0.05). Cumulative seizure duration was reduced in the following order: TBI-vehicle 727 ± 688 s, TBI-TSA 898 ± 937 s, TBI-LEV (54 mg/kg) 358 ± 715 s, TBI-LEV (150 mg/kg) 42 ± 64 (p < 0.05 vs. TBI-vehicle), and TBI-LEV (150 mg/kg) combined with TSA (1 mg/kg) 109 ± 282 s (p < 0.05). This first preclinical intervention study on post-TBI acute seizures shows that a combination therapy with the tissue recovery enhancer TSA and LEV was safe but exhibited no clear benefit over LEV monotherapy on antiseizure efficacy. A longer follow-up is needed to confirm the possible beneficial effects of LEV monotherapy and combination therapy with TSA on chronic post-TBI structural and functional outcomes, including epileptogenesis.
A Systematic Review and Meta-Analysis of Free Triiodothyronine (FT3) Levels in Humans Depending on Seasonal Air Temperature Changes: Is the Variation in FT3 Levels Related to Nonshivering Thermogenesis?
Authors: Nikanorova Alena , Barashkov Nikolay , Pshennikova Vera , Teryutin Fedor , Nakhodkin Sergey , Solovyev Aisen , Romanov Georgii , Burtseva Tatiana , Fedorova Sardana
Published: September 2023

Thyroid hormones play a crucial role in regulating normal development, growth, and metabolic function. However, the controversy surrounding seasonal changes in free triiodothyronine (FT3) levels remains unresolved. Therefore, the aim of this study was to conduct a systematic review and meta-analysis of variations in FT3 levels in relation to seasonal air temperatures in the context of current knowledge about its role in nonshivering thermogenesis. Ten eligible articles with a total of 336,755 participants were included in the meta-analysis. The studies were categorized into two groups based on the air temperature: “Cold winter”, where the winter temperature fell below 0 °C, and “Warm winter”, where the winter temperature was above 0 °C. The analysis revealed that in cold regions, FT3 levels decreased in winter compared to summer (I2 = 57%, p < 0.001), whereas in warm regions, FT3 levels increased during winter (I2 = 28%, p < 0.001). These findings suggest that seasonal variations in FT3 levels are likely to be influenced by the winter temperature. Considering the important role of the FT3 in the nonshivering thermogenesis process, we assume that this observed pattern is probably related to the differences in use of thyroid hormones in the brown adipose tissue during adaptive thermogenesis, which may depend on intensity of cold exposure.
Applications of Genome Editing Technologies in CAD Research and Therapy with a Focus on Atherosclerosis
Authors: Mak Michelle C. E. , Gurung Rijan , Foo Roger S. Y.
Published: September 2023

Cardiovascular diseases, particularly coronary artery disease (CAD), remain the leading cause of death worldwide in recent years, with myocardial infarction (MI) being the most common form of CAD. Atherosclerosis has been highlighted as one of the drivers of CAD, and much research has been carried out to understand and treat this disease. However, there remains much to be better understood and developed in treating this disease. Genome editing technologies have been widely used to establish models of disease as well as to treat various genetic disorders at their root. In this review, we aim to highlight the various ways genome editing technologies can be applied to establish models of atherosclerosis, as well as their therapeutic roles in both atherosclerosis and the clinical implications of CAD.
A Rigid–Flexible and Multi-Siloxane Bridge Strategy for Toughening Epoxy Resin with Promising Flame Retardancy, Mechanical, and Dielectric Properties
Authors: Li Dingsi , Lin Shufeng , Hao Jiahui , He Baohan , Zhang Huagui , Chen Mingfeng
Published: September 2023

Developing highly efficient and multifunctional epoxy resins (EPs) that overcome the shortcomings of flammability and brittleness is crucial for pursuing sustainable and safe application but remains a huge challenge. In this paper, a novel biomass-containing intumescent flame retardant containing a rigid–flexible and multi-siloxane bridge structure (DPB) was synthesized using siloxane; 9,10-dihydro-9-oxa-10-phosphaphenanthrene 10-oxide (DOPO); and biomass vanillin. DPB could facilitate the formation of a carbon residual with an intumescent structure, which effectively blocked the propagation of heat and oxygen. As a result, the peak heat release rate (pHRR) and total heat release (THR) of DPB/EP-7.5 decreased by 38.8% and 45.0%, respectively. In terms of mechanical properties, the tensile and flexural elongations at break of DPB/EP-7.5 increased by 77.2% and 105.3%, respectively. Impressively, DPB/EP-7.5 had excellent dielectric properties, with a dielectric constant of 2.5–2.9. This was due to the Si-O bonds (multi-siloxane bridges) contained in DPB/EP, which can quench the polarization behavior of the hydroxyl group. This paper provides a facile strategy for the preparation of multifunctional EP, which will pave the way for the promotion and application of EP in the high-end field.
Alteration of Neural Network and Hippocampal Slice Activation through Exosomes Derived from 5XFAD Nasal Lavage Fluid
Authors: Kim Sangseong , Jeon Jaekyong , Ganbat Dulguun , Kim Taewoon , Shin Kyusoon , Hong Sungho , Hong Jongwook
Published: September 2023

Exosomes, key mediators of intercellular transmission of pathogenic proteins, such as amyloid-beta and tau, significantly influence the progression and exacerbation of Alzheimer’s disease (AD) pathology. Present in a variety of biological fluids, including cerebrospinal fluid, blood, saliva, and nasal lavage fluid (NLF), exosomes underscore their potential as integral mediators of AD pathology. By serving as vehicles for disease-specific molecules, exosomes could unveil valuable insights into disease identification and progression. This study emphasizes the imperative to investigate the impacts of exosomes on neural networks to enhance our comprehension of intracerebral neuronal communication and its implications for neurological disorders like AD. After harvesting exosomes derived from NLF of 5XFAD mice, we utilized a high-density multielectrode array (HD-MEA) system, the novel technology enabling concurrent recordings from thousands of neurons in primary cortical neuron cultures and organotypic hippocampal slices. The ensuing results revealed a surge in neuronal firing rates and disoriented neural connectivity, reflecting the effects provoked by pathological amyloid-beta oligomer treatment. The local field potentials in the exosome-treated hippocampal brain slices also exhibited aberrant rhythmicity, along with an elevated level of current source density. While this research is an initial exploration, it highlights the potential of exosomes in modulating neural networks under AD conditions and endorses the HD-MEA as an efficacious tool for exosome studies.
Influence of pH on Inulin Conversion to 2,3-Butanediol by Bacillus licheniformis 24: A Gene Expression Assay
Authors: Tsigoriyna Lidia , Arsov Alexander , Gergov Emanoel , Petrova Penka , Petrov Kaloyan
Published: September 2023

2,3-Butanediol (2,3-BD) is an alcohol highly demanded in the chemical, pharmaceutical, and food industries. Its microbial production, safe non-pathogenic producer strains, and suitable substrates have been avidly sought in recent years. The present study investigated 2,3-BD synthesis by the GRAS Bacillus licheniformis 24 using chicory inulin as a cheap and renewable substrate. The process appears to be pH-dependent. At pH 5.25, the synthesis of 2,3-BD was barely detectable due to the lack of inulin hydrolysis. At pH 6.25, 2,3-BD concentration reached 67.5 g/L with rapid hydrolysis of the substrate but was accompanied by exopolysaccharide (EPS) synthesis. Since inulin conversion by bacteria is a complex process and begins with its hydrolysis, the question of the acting enzymes arose. Genome mining revealed that several glycoside hydrolase (GH) enzymes from different CAZy families are involved. Five genes encoding such enzymes in B. licheniformis 24 were amplified and sequenced: sacA, sacB, sacC, levB, and fruA. Real-time RT-PCR experiments showed that the process of inulin hydrolysis is regulated at the level of gene expression, as four genes were significantly overexpressed at pH 6.25. In contrast, the expression of levB remained at the same level at the different pH values at all-time points. It was concluded that the sacC and sacA/fruA genes are crucial for inulin hydrolysis. They encode exoinulinase (EC 3.2.1.80) and sucrases (EC 3.2.1.26), respectively. The striking overexpression of sacB under these conditions led to increased synthesis of EPS; therefore, the simultaneous production of 2,3-BD and EPS cannot be avoided.
Unfolded Protein Response Signaling in Liver Disorders: A 2023 Updated Review
Authors: Shreya Smriti , Grosset Christophe F. , Jain Buddhi Prakash
Published: September 2023

Endoplasmic reticulum (ER) is the site for synthesis and folding of secreted and transmembrane proteins. Disturbance in the functioning of ER leads to the accumulation of unfolded and misfolded proteins, which finally activate the unfolded protein response (UPR) signaling. The three branches of UPR—IRE1 (Inositol requiring enzyme 1), PERK (Protein kinase RNA-activated (PKR)-like ER kinase), and ATF6 (Activating transcription factor 6)—modulate the gene expression pattern through increased expression of chaperones and restore ER homeostasis by enhancing ER protein folding capacity. The liver is a central organ which performs a variety of functions which help in maintaining the overall well-being of our body. The liver plays many roles in cellular physiology, blood homeostasis, and detoxification, and is the main site at which protein synthesis occurs. Disturbance in ER homeostasis is triggered by calcium level imbalance, change in redox status, viral infection, and so on. ER dysfunction and subsequent UPR signaling participate in various hepatic disorders like metabolic (dysfunction) associated fatty liver disease, liver cancer, viral hepatitis, and cholestasis. The exact role of ER stress and UPR signaling in various liver diseases is not fully understood and needs further investigation. Targeting UPR signaling with drugs is the subject of intensive research for therapeutic use in liver diseases. The present review summarizes the role of UPR signaling in liver disorders and describes why UPR regulators are promising therapeutic targets.
Phenolic-Based Discrimination between Non-Symptomatic and Symptomatic Leaves of Aesculus hippocastanum Infested by Cameraria ohridella and Erysiphe flexuosa
Authors: Hanaka Agnieszka , Dresler Sławomir , Mułenko Wiesław , Wójciak Magdalena , Sowa Ireneusz , Sawic Magdalena , Stanisławek Katarzyna , Strzemski Maciej
Published: September 2023

The herbivore Cameraria ohridella (kingdom Animalia) and the pathogen Erysiphe flexuosa (kingdom Fungi) are considered pests and biotic stressors of Aesculus hippocastanum (chestnut trees). The impact of both pests on the accumulation of secondary metabolites in chestnut leaves was investigated. Specifically, the interactive effect of both pests on metabolite accumulation and their potential role in enhancing the resistance of chestnut trees to biological stress was the focus of this study. Aesculus hippocastanum leaves with varying degrees of Cameraria ohridella infestation and Erysiphe flexuosa infection were used in this research. Leaf samples were collected during the plant vegetative growth phase and evaluated for pest infection and secondary metabolite content. Eight main polyphenols were identified in the leaves: (1) neochlorogenic acid, (2) (−)-epicatechin, (3) procyanidin trimer A-type, (4) procyanidin tetramer A-type, (5) quercetin-3-O-arabinoside, (6) quercetin-3-O-rhamnoside, (7) kaempferol-3-O-arabinoside, and (8) kaempferol-3-O-rhamnoside. It was found that the accumulation of metabolites, primarily those derived from epicatechin and quercetin, during the initial vegetation phase (up to 11.05 or 09.05), strongly depended on the later degree of pest infection. The differences observed in the metabolite dynamics in the chestnut leaves, depending on the extent of infection, indicate the development of a metabolic response mechanism in chestnut trees to biological stress.
VHL L169P Variant Does Not Alter Cellular Hypoxia Tension in Clear Cell Renal Cell Carcinoma
Authors: Hu Junhui , Smith Desmond J. , Wu Lily
Published: September 2023

In the current era of tumor genome sequencing, single amino acid missense variants in the von Hippel–Lindau (VHL) tumor suppressor gene are frequently identified in clear cell renal carcinoma (ccRCC). Due to the incomplete knowledge of the structural architecture of VHL protein, the functional significance of many missense mutations cannot be assigned. L169P is one such missense mutation identified in the case of aggressive, metastatic ccRCC. Here, we characterized the biochemical activity, transcriptomic hypoxia signature and biological functions of the L169P variant. Lentiviral vector expressing either wildtype (WT) or L169P VHL were used to transduce two VHL-deficient human ccRCC cell lines, 786-O and RCC4. The stability of the VHL protein and the expression level of VHL, HIF1α and HIF2α were analyzed. The impact of restoring L169P or WT VHL on the hypoxia gene expression program in 786-O cells was assessed by mRNA sequencing (RNAseq) and computed hypoxic scores. The impact of restoring VHL expression on the growth of ccRCC models was assessed in cell cultures and in chorioallantoic membrane (CAM) xenografts. In the 786-O cells, the protein stability of L169P VHL was comparable to WT VHL. No obvious difference in the capability of degrading HIF1α and HIF2α was observed between WT and L169P VHL in the 786-O or RCC4 cells. The hypoxic scores were not significantly different in the 786-O cells expressing either wildtype or L169P VHL. From the cellular function perspective, both WT and L169P VHL slowed cell proliferation in vitro and in vivo. The L169P VHL variant is comparable to WT VHL in terms of protein stability, ability to degrade HIF1α factors and ability to regulate hypoxia gene expression, as well as in the suppression of ccRCC tumor cell growth. Taken together, our data indicate that the L169P VHL variant alone is unlikely to drive the oncogenesis of sporadic ccRCC.
The Anti-Caries Effects of a Novel Peptide on Dentine Caries: An In Vitro Study
Authors: Zhang Olivia Lili , Niu John Yun , Yu Ollie Yiru , Yin Iris Xiaoxue , Mei May Lei , Chu Chun Hung
Published: September 2023

This study aimed to investigate the antibiofilm and remineralising effects of peptide GAPI on artificial dentin caries. After creating artificial carious lesions, eighty dentine blocks were randomly assigned for treatment twice daily with GAPI (GAPI group) or deionised water (control group). Both groups underwent a 7-day biochemical cycle. Scanning electron microscopy (SEM) showed S. mutans with damaged structures that partially covered the dentine in the GAPI group. The dead–live ratios for the GAPI and control groups were 0.77 ± 0.13 and 0.37 ± 0.09 (p < 0.001). The log colony-forming units for the GAPI and control groups were 7.45 ± 0.32 and 8.74 ± 0.50 (p < 0.001), respectively. The lesion depths for the GAPI and control groups were 151 ± 18 µm and 214 ± 15 µm (p < 0.001), respectively. The mineral losses for the GAPI and control groups were 0.91 ± 0.07 gHAcm−3 and 1.01 ± 0.07 gHAcm−3 (p = 0.01), respectively. The hydrogen-to-amide I ratios for the GAPI and control groups were 2.92 ± 0.82 and 1.83 ± 0.73 (p = 0.014), respectively. SEM micrographs revealed fewer exposed dentine collagen fibres in the GAPI group compared to those in the control group. Furthermore, X-ray diffraction (XRD) patterns indicated that the hydroxyapatite in the GAPI group was more crystallised than that in the control group. This study demonstrated GAPI’s antibiofilm and remineralising effects on artificial dentin caries.
Hierarchical Ni-Mn LDHs@CuC2O4 Nanosheet Arrays-Modified Copper Mesh: A Dual-Functional Material for Enhancing Oil/Water Separation and Supercapacitors
Authors: Wu Yue , Lu Guangyuan , Xu Ping , Zhang Tian C. , He Huaqiang , Yuan Shaojun
Published: September 2023

The pursuit of superhydrophilic materials with hierarchical structures has garnered significant attention across diverse application domains. In this study, we have successfully crafted Ni-Mn LDHs@CuC2O4 nanosheet arrays on a copper mesh (CM) through a synergistic process involving chemical oxidation and hydrothermal deposition. Initially, CuC2O4 nanosheets were synthesized on the copper mesh, closely followed by the growth of Ni-Mn LDHs nanosheets, culminating in the establishment of a multi-tiered surface architecture with exceptional superhydrophilicity and remarkable underwater superoleophobicity. The resultant Ni-Mn LDHs@CuC2O4 CM membrane showcased an unparalleled amalgamation of traits, including superhydrophilicity, underwater superoleophobicity, and the ability to harness photocatalytic forces for self-cleaning actions, making it an advanced oil-water separation membrane. The membrane’s performance was impressive, manifesting in a remarkable water flux range (70 kL·m−2·h−1) and an efficient oil separation capability for both oil/water mixture and surfactant-stabilized emulsions (below 60 ppm). Moreover, the innate superhydrophilic characteristics of the membrane rendered it a prime candidate for deployment as a supercapacitor cathode material. Evidenced by a capacitance of 5080 mF·cm−2 at a current density of 6 mA cm−2 in a 6 M KOH electrolyte, the membrane’s potential extended beyond oil-water separation. This work not only introduces a cutting-edge oil-water separation membrane and supercapacitor electrode but also offers a promising blueprint for the deliberate engineering of hierarchical structure arrays to cater to a spectrum of related applications.
Effects of Coloration of Spinel CoAl2O4 Cobalt Blue Pigments: Composition, Structure, and Cation Distribution
Authors: Zhang Weiran , Li Ziyu , Wu Guohua , Wu Wei , Zeng Hailan , Jiang Haiyun , Zhang Weili , Wu Ruomei , Xue Qiong
Published: September 2023

Cobalt blue ceramic pigments mainly consisting of CoAl2O4 are subject to the difficulty of color control. Here, a perspective is reported regarding research on the reasons for color change based on the control of the heat treatment and ratio of components. Macroscopically, the composition of pigment powders determines the color. Microscopically, the crystallite characters including size, cation distribution, and structure have an important effect on the color. The ingredient, structural, and color properties of the pigment powders are analyzed using thermo gravimetry–differential scanning calorimetry (TG–DSC), X-ray diffraction (XRD) measurement, Rietveld refinement, energy dispersive spectrometer (EDS), and colorimetry analysis. The color is proven to be associated with cation distribution, such as that of Co2+ and Co3+. It is indicated that high heating temperature, long heating time, and a large proportion of Al3+ can, respectively, induce the Co2+ and Al3+ in tetrahedral and octahedral sites.
Thermal Image Processing for Respiratory Estimation from Cubical Data with Expandable Depth
Authors: Szankin Maciej , Kwasniewska Alicja , Ruminski Jacek
Published: September 2023

As healthcare costs continue to rise, finding affordable and non-invasive ways to monitor vital signs is increasingly important. One of the key metrics for assessing overall health and identifying potential issues early on is respiratory rate (RR). Most of the existing methods require multiple steps that consist of image and signal processing. This might be difficult to deploy on edge devices that often do not have specialized digital signal processors (DSP). Therefore, the goal of this study is to develop a single neural network realizing the entire process of RR estimation in a single forward pass. The proposed solution builds on recent advances in video recognition, capturing both spatial and temporal information in a multi-path network. Both paths process the data at different sampling rates to capture rapid and slow changes that are associated with differences in the temperature of the nostril area during the breathing episodes. The preliminary results show that the introduced end-to-end solution achieves better performance compared to state-of-the-art methods, without requiring additional pre/post-processing steps and signal-processing techniques. In addition, the presented results demonstrate its robustness on low-resolution thermal video sequences that are often used at the embedded edge due to the size and power constraints of such systems. Taking that into account, the proposed approach has the potential for efficient and convenient respiratory rate estimation across various markets in solutions deployed locally, close to end users.
Conocybe Section Pilosellae in China: Reconciliation of Taxonomy and Phylogeny Reveals Seven New Species and a New Record
Authors: Song Han-Bing , Bau Tolgor
Published: September 2023

Conocybe belongs to the Bolbitiaceae. The morphological classification and molecular phylogenetics of Conocybe section Pilosellae are not in agreement. In this study, based on the specimens from China, we investigated the sect. Pilosellae and identified 17 species, including 7 new species: Conocybe pilosa, with a densely hairy pileus and stipe; C. reniformis, with reniform spores; C. ceracea, with waxy dehydration of the lamellae; C. muscicola, growing on moss; C. sinobispora, with two-spored basidia; C. hydrophila, with a hygrophanous pileus; C. rufostipes, growing on dung with a brown stipe; and C. pseudocrispa, one new record for China. A key was compiled for the sect. Pilosellae in China. Here, the sect. Pilosellae, and new species and records from China are morphologically described and illustrated. Maximum likelihood and Bayesian analyses were performed using a combined nuc rDNA internal transcribed spacer region (ITS) and nuc 28S rDNA (nrLSU), and translation elongation factor 1-alpha (tef1-α) dataset to reconstruct the relationships of this section. We found that the sect. Pilosellae was the basal clade of Conocybe, and its evolutionary features may shed light on the characteristics of Conocybe. By integrating morphological classification and phylogenetic analysis, we explored the possible phylogenetic relationships among the species of the sect. Pilosellae in China.
Influence of the Extraction Solution on the Removal of Heavy Metals from Polluted Soils
Authors: Sur Ioana Monica , Hegyi Andreea , Micle Valer , Gabor Timea , Lăzărescu Adrian-Victor
Published: September 2023

Soil pollution with heavy metals is a problem for the whole geosystem. The aim of the research is to identify new solutions for extracting heavy metals from polluted soils so that they can be further exploited. To this end, investigations of the physicochemical characteristics of the soil sample under study were carried out. Following the analyses, the soil was characterised as lute-coarse sand (UG) with a strongly acidic pH (4.67), a hygroscopicity coefficient (CH = 4.8% g/g), and a good supply of nutrients: nitrogen (Nt): 0.107%; mobile phosphorus (PAL): 6 mg kg−1 and mobile potassium (KAL): 26 mg kg−1, but is low in humus (2.12%). The metal content of the soil was determined by atomic absorption spectrometry (AAS), and the analyses showed high concentrations of metals (Pb: 27,660 mg kg−1; Cu: 5590 mg kg−1; Zn: 2199 mg kg−1; Cd: 11.68 mg kg−1; Cr: 146 mg kg−1). The removal of metals (Pb, Cu, Zn, Cd, and Cr) from polluted soil by different extraction agents (water, humus, malic acid, chitosan, and gluconic acid) was investigated. Metal extraction experiments were carried out in a continuous orbital rotation-oscillation stirrer at a solid/liquid/ (S/L ratio; g:mL) of 1:4, at two concentrations of extraction solution (1% and 3%), and at different stirring times (2, 4, 6, and 8 h). The yield of the extraction process is very low for all proposed extraction solutions. The maximum values of extraction efficiency are: 0.5% (Pb); 3.28% (Zn); and 5.72% (Cu). Higher values were obtained in the case of Cr (11.97%) in the variant of using humus 3% as an extraction solution at a stirring time of 6 h. In the investigated experimental conditions, the best removal efficiencies were obtained in the case of cadmium (26.71%) when using a 3% malic acid solution. In conclusion, it is considered that, from case to case, the type of extraction solution as well as the nature of the metal influence the mechanism of the depollution process, i.e., the capacity of the fine soil granules to free themselves from the pollutant metal that has adhered to them, and further research is considered necessary in the future.
Effect of Metallic Coatings on the Wear Performance and Mechanism of 30CrMnSiNi2A Steel
Authors: Zu Huicheng , He Zhiqiang , He Bin , Tang Zhuoquan , Fang Xiuyang , Cai Zhenbing , Cao Zhongqing , An Luling
Published: September 2023

The finger lock structure of aircraft landing gear is prone to wear and failure during repeated locking and unlocking processes, which is disastrous for the service safety of the aircraft. At present, the commonly used material for finger locks in the industry is 30CrMnSiNi2A, which has a short wear life and high maintenance costs. It is crucial to develop effective methods to improve the wear resistance of 30CrMnSiNi2A finger locks. This work explores the wear resistance and wear mechanisms of different metallic coatings such as chromium, nickel, and cadmium–titanium on the surface of a 30CrMnSiNi2A substrate. The effects of load and wear time on the wear behavior are also discussed. The results indicated that the wear resistance of the chromium coating was the maximum. When the load was 80 N and 120 N, the wear mechanisms were mainly oxidation and adhesive. For greater loads, the wear mechanism of the coating after failure was mainly abrasive and oxidation, and the wear was extremely severe. When the load was 80 N, for a greater loading time, the wear mechanisms were mainly oxidation and adhesive.
Effects of Alloying Elements and Mechanical Alloying on Characteristics of WVTaTiCr Refractory High-Entropy Alloys
Authors: Chen Chun-Liang , Lin Jyun-Hong
Published: September 2023

Refractory high-entropy alloys (RHEAs) are among the promising candidates for the design of structural materials in advanced nuclear energy systems. The effects of Cr, V, Ta, and Ti elements and ball milling on the microstructural evolution and mechanical properties of model RHEAs were investigated. The results show that W-rich BCC1 and Ta-rich BCC2 solid solution phases were generated after a long milling duration. After high-temperature sintering, the (Cr, Ta)-rich phase associated with the Laves phase was observed in the Cr-containing model RHEAs. In addition, a high level of Ti, Ta, and V contents promoted the in situ formation of oxide particles in the alloys. Complex TiTa2O7 and Ta2VO6 oxide phases were identified by TEM, which suggests a solid-state reaction of Ti-O, Ta-O, and V-O subjected to high-energy ball milling. The oxide particles are uniformly dispersed in the BCC matrix, which can result in dispersion strengthening and the enhancement of mechanical properties.
Recycling Local Waste Glass Bottles into Cement Paste: Effect on Hydration, Microstructure, and CO2 Emission
Authors: Ren Fengming , Zhang Xiwen , Lin Mingxin , Wang Qing , Sun Jing
Published: September 2023

Large amounts of waste glass are generated along with the manufacturing of glass products, causing detrimental effects on the environment. Through crushing and ball-milling, waste glass powder (WGP) can be acquired from glass bottles and has been suggested in cementitious systems due to its potential pozzolanic activity. To better understand the impact of WGP on cementitious composites, experimental tests of rheology, heat of hydration, and strength development were conducted on cement pastes with and without WGP. Results show that the rheological performance of cement paste is improved when WGP with particles passing through 80 μm sieves is incorporated. The retarding effect and pozzolanic reaction were observed through X-ray diffraction patterns and thermo-gravimetric parameter analyses. A calcium hydroxide (CH) content calculation further confirms the secondary reactivity of WGP in cement pastes. Compared with the samples without WGP, the normalized CH content of binder per unit mass containing 35% WGP decreased by 21.01%, 24.94%, and 27.41% at the ages of 1, 28, and 90 days, respectively, which contributes to late-age strength development of pastes. At the same time, the hydration per unit of cement was increased by 21.53%, 15.48%, and 11.68%, which improved the cement efficiency. In addition, WGP particles provide nuclei for hydration products, facilitating the subsequent growth of C-S-H and strength development in late ages. Based on value engineering analysis, WGP was found to reduce the impact of Portland cement on the environment by 34.9% in terms of carbon dioxide emissions, indicating a bright prospect for WGP in the cement industry.