bluuebunny/crossref_metadata_2025 huggingface dataset:
Facing COVID-19 Between Sensory and Psychoemotional Stress, and Instrumental Deprivation: A Qualitative Study of Unmanageable Critical Incidents With Doctors and Nurses in Two Hospitals in Northern Italy
Authors: Testoni Ines , Franco Chiara , Gallo Stampino Enrica , Iacona Erika , Crupi Robert , Pagano Claudio
Published: April 2021

Background: The COVID-19 pandemic severely strained the already unprepared Italian healthcare system. This had repercussions on healthcare workers, stemming, in particular, from a lack of clear guidelines, adequate protective equipment, and professional preparedness. Such conditions were especially prevalent in Northern Italy.

Objectives: This study aimed to examine COVID-19-related professional and psychoemotional stress among nurses and doctors in two hospitals in Northern Italy, along with the worst critical incidents affecting healthcare personnel. A parallel objective was to elicit healthcare professionals' opinions about what changes are needed in the healthcare system's operations, as well as about the relational/emotional skills that are needed to better manage relationships with patients in emergency situations.

Participants: Snowball sampling was used to recruit participants and yielded 17 hospital professionals: six nurses (five female and one male) and 11 doctors (seven male and four female). Three of these professionals worked in intensive care and the others in different wards. All had close contact with COVID-19 patients.

Methods: The study employed a qualitative research design, using in-depth interviews of ~60 min each that were conducted via Skype video calls. The interviews were recorded and transcribed, then analysed. The qualitative analysis employed mixed methods to identify the most relevant and recursive themes from the interviews.

Results: Four fundamental themes emerged from our analysis of the interview texts: (1) disorganisation and psychoemotional stress; (2) urgency and critical incidents; (3) everything surreal; and (4) disruptions in empathetic relationships with patients.

Conclusions: Through our analysis of the interview narratives, we found that systematic and in-depth psychological training is needed to prepare professionals for (1) altered relationships with patients in emergencies; (2) use of exceptional medical equipment; (3) elaboration of new bioethical models suitable for disasters and pandemics; and (4) engagement with the themes of death and dying.
Differences and Similarities in the Contributions of Phonological Awareness, Orthographic Knowledge and Semantic Competence to Reading Fluency in Chinese School-Age Children With and Without Hearing Loss
Authors: Zhang Linjun , Hong Tian , Li Yu , Wang Jiuju , Zhang Yang , Shu Hua
Published: April 2021

Compared with the large number of studies on reading of children with hearing loss (HL) in alphabetic languages, there are only a very limited number of studies on reading of Chinese-speaking children with HL. It remains unclear how phonological, orthographic, and semantic skills contribute to reading fluency of Chinese school-age children with HL. The present study explored this issue by examining the performances of children with HL on reading fluency and three linguistic skills compared with matched controls with normal hearing (NH). Specifically, twenty-eight children with HL and 28 chronological-age-matched children with NH were tested on word/sentence reading fluency (WRF/SRF), phonological awareness (PA) which was composed of onset/vowel/lexical tone awareness, orthographic knowledge (OK), and semantic competence (SC) which comprised animal word identification, pseudo-homophone detection, and word segmentation. Results showed that children with HL lagged behind their peers with NH in WRF/SRF and most of the phonological, orthographic, and semantic subskills except onset awareness and pseudo-homophone detection. Furthermore, the significant contributors to WRF differed between the two groups with PA being the significant contributor in the children with NH while OK being the significant contributor in the children with HL. However, the significant contributor to SRF did not differ between the two groups with SC being the only significant contributor. These results revealed not only between-group differences but also similarities in the relative contributions of PA, OK, and SC to reading fluency at both word and sentence levels, which has practical implications for developing better training programs to improve reading for children with HL.
Workload, Techno Overload, and Behavioral Stress During COVID-19 Emergency: The Role of Job Crafting in Remote Workers
Authors: Ingusci Emanuela , Signore Fulvio , Giancaspro Maria Luisa , Manuti Amelia , Molino Monica , Russo Vincenzo , Zito Margherita , Cortese Claudio Giovanni
Published: April 2021

The radical changes deriving from the COVID-19 emergency have heavily upset some of the most familiar routines of daily work life. Abruptly, many workers have been forced to face the difficulties that come with switching to remote working. Basing on the theoretical framework proposed by the Job Demands-Resources model, the purpose of this paper was to explore the effect of work overload (workload and techno overload), on behavioral stress, meant as an outcome linked to the health impairment process. Furthermore, the aim of the study was to explore the mediating role of job crafting, considered as a second-order construct consisting of two dimensions (increasing structural resources and increasing challenging demands) in the abovementioned relation. Participants were 530 workers experiencing remote working or work-from-home during the first COVID-19 lockdown in Italy (March–May 2020). Hypotheses were explored by using three different latent variables, measured reflexively through indicators on a 5-point scale, extracted from validated questionnaires. Data analysis was performed through Structural Equation Modeling; to test the mediation, bootstrap validation was computed (n = 2,000). Results showed that the mediation of job crafting was partial. More specifically, the direct effect between work overload and behavioral stress was positive; moreover, the indirect, negative effect through the mediation of job crafting was also significant. Therefore, results showed that job crafting can play a crucial role as a protective factor supporting the activation and adjustment of suitable resources; these resources can be useful to deal with the negative effects of work overload, particularly under the condition of heavy remote working and use of technologies, on individual outcomes. Starting from the current global scenario of the pandemic that has not yet ceased its effects, the study suggested decisive theoretical and practical implications. Accordingly, findings extended the current trends in occupational health psychology research, with special reference to the mainstream topic “work and COVID-19” in the Italian context. Finally, results can give suggestions to companies engaged in managing change, recommending that they build a collaborative workplace at the individual and collective level to implement job crafting interventions and enrich the personal and organizational resources of workers, which is useful cope with the current demands.
Under-Reporting of COVID-19 Cases Among Indigenous Peoples in Brazil: A New Expression of Old Inequalities
Authors: Fellows Martha , Paye Valéria , Alencar Ane , Nicácio Mário , Castro Isabel , Coelho Maria Emília , Silva Camila V. J. , Bandeira Matheus , Lourival Reinaldo , Basta Paulo Cesar
Published: April 2021

Objective: To estimate the incidence, mortality and lethality rates of COVID-19 among Indigenous Peoples in the Brazilian Amazon. Additionally, to analyze how external threats can contribute to spread the disease in Indigenous Lands (IL).

Methods: The Brazilian Amazon is home to nearly half a million Indigenous persons, representing more than 170 ethnic groups. As a pioneer in heading Indigenous community-based surveillance (I-CBS) in Brazil, the Coordination of the Indigenous Organizations of the Brazilian Amazon (COIAB) started to monitor Indigenous COVID-19 cases in March of 2020. Brazil's Ministry of Health (MOH) was the main source of data regarding non-Indigenous cases and deaths; to contrast the government's tally, we used the information collected by I-CBS covering 25 Special Indigenous Sanitary Districts (DSEI) in the Brazilian Amazon. The incidence and mortality rates of COVID-19 were calculated using the total number of new cases and deaths accumulated between the 9th and 40th epidemiological weeks. We studied (a) the availability of health care facilities to attend to Indigenous Peoples; (b) illegal mines, land grabbing, and deforestation to perform a geospatial analysis to assess how external threats affect Indigenous incidence and mortality rates. We used the Generalized Linear Model (GLM) with Poisson regression to show the results.

Results: MOH registered 22,127 cases and 330 deaths, while COIAB's survey recorded 25,356 confirmed cases and 670 deaths, indicating an under-reporting of 14 and 103%, respectively. Likewise, the incidence and mortality rates were 136 and 110% higher among Indigenous when compared with the national average. In terms of mortality, the most critical DSEIs were Alto Rio Solimões, Cuiabá, Xavante, Vilhena and Kaiapó do Pará. The GLM model reveals a direct correlation between deforestation, land grabbing and mining, and the incidence of cases among the Indigenous.

Conclusion: Through this investigation it was possible to verify that not only the incidence and mortality rates due to COVID-19 among Indigenous Peoples are higher than those observed in the general population, but also that the data presented by the federal government are underreported. Additionally, it was evident that the presence of illegal economic activities increased the risk of spreading COVID-19 in ILs.
Considerations for Pairing Cognitive Behavioral Therapies and Non-invasive Brain Stimulation: Ignore at Your Own Risk
Authors: Conelea Christine A. , Jacob Suma , Redish A. David , Ramsay Ian S.
Published: April 2021

Multimodal approaches combining cognitive behavioral therapies (CBT) with non-invasive brain stimulation (NIBS) hold promise for improving the treatment of neuropsychiatric disorders. As this is a relatively new approach, it is a critical time to identify guiding principles and methodological considerations to enhance research rigor. In the current paper, we argue for a principled approach to CBT and NIBS pairings based on synergistic activation of neural circuits and identify key considerations about CBT that may influence pairing with NIBS. Careful consideration of brain-state interactions and CBT-related nuances will increase the potential for these combinations to be positively synergistic.
Societal Impacts of Pandemics: Comparing COVID-19 With History to Focus Our Response
Authors: Patterson Grace E. , McIntyre K. Marie , Clough Helen E. , Rushton Jonathan
Published: April 2021

COVID-19 has disrupted everyday life worldwide and is the first disease event since the 1918 H1N1 Spanish influenza (flu) pandemic to demand an urgent global healthcare response. There has been much debate on whether the damage of COVID-19 is due predominantly to the pathogen itself or our response to it. We compare SARS-CoV-2 against three other major pandemics (1347 Black Death, 1520's new world smallpox outbreaks, and 1918 Spanish Flu pandemic) over the course of 700 years to unearth similarities and differences in pathogen, social and medical context, human response and behavior, and long-term social and economic impact that should be used to shape COVID-19 decision-making. We conclude that <100 years ago, pandemic disease events were still largely uncontrolled and unexplained. The extensive damage wreaked by historical pandemics on health, economy, and society was a function of pathogen characteristics and lack of public health resources. Though there remain many similarities in patterns of disease spread and response from 1300 onwards, the major risks posed by COVID-19 arise not from the pathogen, but from indirect effects of control measures on health and core societal activities. Our understanding of the epidemiology and effective treatment of this virus has rapidly improved and attention is shifting toward the identification of long-term control strategies that balance consideration of health in at risk populations, societal behavior, and economic impact. Policymakers should use lessons from previous pandemics to develop appropriate risk assessments and control plans for now-endemic COVID-19, and for future pandemics.
COVID-19 Research: Challenges to Interpret Numbers and Propose Solutions
Authors: Struelens Marc J. , Vineis Paolo
Published: April 2021

The response of the scientific community to the COVID-19 pandemic has been unprecedented in size, speed and discovery output. Within months of virus emergence, the SARS-CoV-2 genomics, replication, evolution and dissemination dynamics as well as natural history, infection risk and prognostic factors and biology of the disease have been gradually deciphered. More than 250 articles on COVID-19 published in Frontiers in Public Health have contributed to these insights. We discuss here some of the key research themes and challenges that have been addressed. We provide our perspective on current research issues with surveillance data quality and limitations of epidemiological methods. We warn against the potential misuse or misleading interpretation of public data of variable quality and the use of inadequate study designs for the evaluation of effect of non-pharmaceutical interventions. We conclude by interrogating possible public health strategies for pandemic control as well as discuss the ethical responsibilities and democratic accountability of researchers in their role as experts and policy advisors.
Inclusive Finance, Environmental Regulation, and Public Health in China: Lessons for the COVID-19 Pandemic
Authors: Liu Xia , Guo Suqin
Published: April 2021

The slow-down of the Chinese economy and the depression in the global economy during the COVID-19 show that governments should provide stimulus packages. These policies should be inclusive in terms of financial gains. Using the panel data of 30 regions in China from 2006 to 2016, this paper uses the Poisson Pseudo-Maximum Likelihood (PPML) estimator to analyze the impact of inclusive finance on public health. The results show that inclusive finance has a significant positive effect on public health. The performance of the eastern region is significantly better than that of the central and western regions. When we consider the combined effect of environmental regulation, the improvement effect of inclusive finance on public health is still significant, and the coefficient increases in the eastern region. Similarly, there is also a significant improvement effect in the central and western regions. Our findings reveal that environmental regulation promotes the beneficial effect of inclusive finance. Therefore, it is important to improve the inclusive financial development mechanism and enhance environmental regulation intensity for solving public health issues. Lessons related to the COVID-19 pandemic are also discussed.
A Stalled Revolution? Misoprostol and the Pharmaceuticalization of Reproductive Health in Francophone Africa
Authors: Suh Siri
Published: April 2021

Misoprostol entered the global market under the name Cytotec in the mid-1980s for the treatment of gastric ulcers. Decades of research have since demonstrated the safety and effectiveness of off-label use of misoprostol as a uterotonic in pregnant women to prevent and treat post-partum hemorrhage, treat incomplete abortion, or terminate first-trimester pregnancy. Global health experts emphasize misoprostol’s potential to revolutionize access to reproductive health care in developing countries. Misoprostol does not require refrigeration, can be self-administered or with the aid of a non-physician, and is relatively inexpensive. It holds particular promise for improving reproductive health in sub-Saharan Africa, where most global maternal mortality related to post-partum hemorrhage and unsafe abortion occurs. Although misoprostol has been widely recognized as an essential obstetric medication, its application remains highly contested precisely because it disrupts medical and legal authority over pregnancy, delivery, and abortion. I draw on fieldwork in Francophone Africa to explore how global health organizations have negotiated misoprostol’s abortifacient qualities in their reproductive health work. I focus on this region not only because it has some of the world’s highest rates of maternal mortality, but also fertility, thereby situating misoprostol in a longer history of family planning programs in a region designated as a zone of overpopulation since the 1980s. Findings suggest that stakeholders adopt strategies that directly address safe abortion on the one hand, and integrate misoprostol into existing clinical protocols and pharmaceutical supply systems for legal obstetric indications on the other. Although misoprostol has generated important partnerships among regional stakeholders invested in reducing fertility and maternal mortality, the stigma of abortion stalls its integration into routine obstetric care and availability to the public. I demonstrate the promises and pitfalls of pharmaceuticalizing reproductive health: despite the availability of misoprostol in some health facilities and pharmacies, low-income and rural women continue to lack access not only to the drug, but to quality reproductive health care more generally.
Heart Rate Changes Before, During, and After Treadmill Walking Exercise in Normal Dogs
Authors: Shull Sarah A. , Rich Sarah K. , Gillette Robert L. , Manfredi Jane M.
Published: April 2021

In dogs, changes in heart rate (HR) can reflect conditioning, fear, anticipation, and pain; however, these are not routinely assessed in veterinary rehabilitation patients. Knowing the expected HR changes during rehabilitation exercises can guide protocols and can optimize post-operative therapy. The primary objectives of the study were to assess HR in dogs undergoing treadmill exercise (TE) during the walk and to compare the three collection techniques of HR, namely, auscultation, a HR monitor (HR MONITOR), and a Holter monitor (HOLTER). We hypothesized that the HR would increase by 20% during TE, that HR taken after TE would not be the same as the HR during TE, and that all methods of measurement would have good agreement. HR was recorded in all methods simultaneously, in eight adult healthy large breed dogs during rest (REST), immediately before TE (PRE), during TE (WALK), and 15 and 60 s after TE (POST-15, POST-60). Statistical analyses included Spearman and Pearson correlations, Bland-Altman analyses, and a repeated measures ANOVA with Sidak's post-hoc test (significant at value of p < 0.05). Increased HR was reflected in TE during WALK, and elevations in HR during WALK were not reflected in POST timepoints. Auscultation was also not possible during WALK. Significant moderate-to-strong correlations existed among all monitoring options at each of the timepoints (rho range = 0.5–0.9, p < 0.05). There were no correlations between peak HR and age or weight. The main limitation of this study is that only healthy and large breed dogs were used. Both monitors captured the increase in HR during exercise and could guide TE regimens to minimize patient risk of injury and to maximize training effectiveness.
An Empirical Analysis on the Longevity of Dairy Cows in Relation to Economic Herd Performance
Authors: Vredenberg Imke , Han Ruozhu , Mourits Monique , Hogeveen Henk , Steeneveld Wilma
Published: April 2021

Several studies have stated the various effects of an increased dairy cow longevity on economic herd performance, but empirical studies are lacking. This study aimed to investigate the association between longevity of dairy cows and the economic performance of dairy herds based on longitudinal Dutch accounting data. Herd and farm accounting data (n= 855 herds) over the years 2007–2016 were analyzed. Herd data contained yearly averages on longevity features, herd size and several production variables. Longevity was defined as the age of cows at culling and by lifetime milk production of culled cows. Farm accounting data contained yearly averages on revenues, fixed and variable costs of the herds, by which gross margins were defined. Data was analyzed using generalized linear mixed modeling, with gross margin as dependent variable. The independent variables consisted of average age of culled cows, average lifetime production of culled cows, year, herd size, herd intensity (milk production per ha), herd expansion rate, soil type, milking system, successor availability, total full-time equivalent, heifer ratio (% of heifers per cow) and use of outsourced heifer rearing. Herd was included as a random effect to account for the heterogeneity among herds. Descriptive statistics showed that the average age of culled cows was 5.87 (STD = 0.78) years and the average lifetime milk production of culled cows was 31.87 (STD = 7.56) tons per cow with an average herd size of 89 cows (STD = 38.85). The average age of culled cows was stable over the 10 years (variation between 5.79 AND 5.90 years). The gross margin was on average €24.80/100 kg milk (STD = 4.67), with the lowest value in year 2009 and the highest value in year 2013. Gross margin was not significantly associated with age of culled cows and lifetime milk production of culled cows. Variance in longevity between herds was large (STD = 0.78 years) but herds with a higher longevity did not perform economically better nor worse than herds resulting in lower longevity. This indicates that, within current practice, there is potential for improving longevity in order to meet society's concerns on animal welfare and environmental pollution, without affecting the economic performance of the herd.
Treatment of Pesticide-Contaminated Water Using a Selected Fungal Consortium: Study in a Batch and Packed-Bed Bioreactor
Authors: Levio-Raiman Marcela , Briceño Gabriela , Leiva Bárbara , López Sebastián , Schalchli Heidi , Lamilla Claudio , Bornhardt Cristian , Diez M. Cristina
Published: April 2021

This study provides the basis for implementing a continuous treatment system for wastewater containing a pesticide mixture formed by atrazine, iprodione, and chlorpyrifos. Two fungal strains (Verticilium sp. H5 and Metacordyceps sp. H12) isolated from a biomixture of a biopurification system were able to remove different pesticide concentrations (10 to 50 mg L−1) efficiently from the liquid medium; however, the half-life of the pesticides was reduced and characterized by a T1/2 of 5.4 to 9.2 d for atrazine, 3.7 to 5.8 d for iprodione, and 2.6 to 2.9 d for chlorpyrifos using the fungal consortium. The immobilization of the fungal consortium in alginate bead was effective, with the highest pesticide removal observed using an inoculum concentration of 30% wv−1. The packed-bed reactor with the immobilized fungal consortium, which was operated in the continuous mode at different flow rates (30, 60, and 90 mL h−1), required approximately 10 d to achieve removal efficiency (atrazine: 59%; iprodione: 96%; chlorpyrifos: ~85%). The bioreactor was sensitive to flow rate fluctuations but was able to recover performance quickly. The pesticide metabolites hydroxyatrazine, 3,5-dichloroaniline, and 3,5,6-trichloro-2-pyridinol were produced, and a slight accumulation of 3,5,6-trichloro-2-pyridinol was observed. Nevertheless, reactor removal efficiency was maintained until the study ended (60 d).
Defining Integrated Weed Management: A Novel Conceptual Framework for Models
Authors: Storkey Jonathan , Helps Joseph , Hull Richard , Milne Alice E. , Metcalfe Helen
Published: April 2021

Weed population dynamics models are an important tool for predicting the outcome of alternative Integrated Weed Management (IWM) scenarios. The growing problem of herbicide resistance has increased the urgency for these tools in the design of sustainable IWM solutions. We developed a conceptual framework for defining IWM as a standardised input template to allow output from different models to be compared and to design IWM scenarios. The framework could also be used as a quantitative metric to determine whether more diverse systems are more sustainable and less vulnerable to herbicide resistance using empirical data. Using the logic of object-oriented programming, we defined four classes of weed management options based on the stage in the weed life cycle that they impact and processes that mediate their effects. Objects in the same class share a common set of properties that determine their behaviour in weed population dynamics models. Any weed control “event” in a system is associated with an object, meaning alternative management scenarios can be built by systematically adding events to a model either to compare existing systems or design novel approaches. Our framework is designed to be generic, allowing IWM systems from different cropping systems and countries to be compared.
Increasing Effective Use of Straw-Derived Nitrogen by Alternate Wetting/Drying Irrigation Combined with N Fertilization Addition in a Soil–Rice System
Authors: Zhang Jianwei , Zhou Yan , Li Weiwei , Nadeem Muhammad Y. , Ding Yanfeng , Jiang Yu , Chen Lin , Li Ganghua
Published: April 2021

Straw-derived N (Straw-N) is an important organic N source, but its distribution in soil–rice systems regulated by water management and N fertilization is poorly understood. Therefore, a pot experiment using 15N-labeled wheat residue was conducted with conventional flooded irrigation (CF) and alternate wetting/drying irrigation (AWD) both with and without N fertilization. Results showed that the whole-plant straw–N recovery rate and the soil residue rate were 9.2–11.9% and 33.5–43.1%, and 10.2–13.8% and 33.7–70.2% at panicle initiation stage (PI) and mature stage (MS), respectively. There was no interaction between water management and N fertilization. Compared to CF, AWD did not affect whole-plant straw-N absorption and significantly changed its distribution in various plant parts, such as increasing the straw-N accumulation in roots at PI and decreasing it at MS. N fertilization addition markedly promoted the transfer of straw-N to the plant but reduced the contribution rate of N uptake by the plant. Furthermore, AWD or N fertilization addition allowed more straw-N to remain in the soil, and a positive interaction effect on the straw-N loss mitigation was found. These results suggest that AWD combined with N fertilization addition is a great measure to improve the efficient utilization of straw-N and avoid the risk of environmental pollution in a soil–rice system.
Comparison of Three Feeding Regimens on Blood Fatty Acids Metabolites of Wujumqin Sheep in Inner Mongolia
Authors: Jin Yanmei , Zhang Xiaoqing , Zhang Jize , Zhang Qian , Tana
Published: April 2021

Feeding regimens influence the fatty acid composition of animal-derived products. However, there is limited information on the effect of feeding regimens on the blood fatty acid composition and metabolic pathways of ruminant animals. In this study, 30 Wujumqin sheep were randomly assigned to three groups, PF (pasture feeding), PSF (pasture feeding plus corn supplementation) and BF (barn feeding), to examine the effects of feeding regimens on blood fatty acid composition and metabolic pathways through a metabolomic approach. The results showed that the BF sheep had increased serum n-6 polyunsaturated fatty acids levels, while the PF and PSF sheep had increased serum n-3 PUFA levels. Compared to the BF and PSF sheep that were fed ground corn, the PF sheep that only ate natural grass had up-regulated serum DHA levels. Meanwhile, blood metabolites from linoleic acid and arachidonic acid, including pro-inflammatory products (20-HETE, LTs, TX etc.) and anti-inflammatory products (LXB4, DHETs, HPETEs etc.) were elevated in the BF group. It was found that, compared to grazing, concentrate supplement feeding regimens, including either grazing plus supplements or feeding indoors, down-regulated blood n-3 PUFA biosynthesis and up-regulated the blood inflammatory compound metabolism by n-6 PUFA.
Flow Cytometric Assessment of the Viability and Functionality of Uterine Polymorphonuclear Leukocytes in Postpartum Dairy Cows
Authors: Lietaer Leen , Demeyere Kristel , Heirbaut Stijn , Meyer Evelyne , Opsomer Geert , Bogado Pascottini Osvaldo
Published: April 2021

Postpartum dairy cows experience impaired peripheral polymorphonuclear leukocyte (PMN) functionality, which has been associated with reproductive tract inflammatory diseases. However, it has not been elucidated yet whether endometrial PMN functionality is (equally) impaired. We developed a method for endometrial PMN isolation and flow cytometric assessment of their viability and functionality. We also evaluated PMN immunolabeling, using a specific bovine granulocyte marker, CH138A. Blood and endometrial cytobrush samples were collected in duplicate from seventeen clinically healthy Holstein-Friesian cows between 9 and 37 days in milk. The proportion of viable, apoptotic, and necrotic PMN in endometrial samples roughly ranged from 10 to 80%, indicating highly dynamic endometrial PMN populations in the postpartum uteri. Endometrial PMN functionality testing revealed that PMN immunolabeling increased the accuracy, although this protocol might influence the median fluorescence intensity of the sample. Phagocytosis seemed the most stable and reliable endometrial PMN function and could be assessed satisfactorily without prior CH138A immunolabeling. However, the interpretation of oxidative burst and intracellular proteolysis tests remains challenging. The correlation between peripheral and endometrial PMN functionality was poor. Further research is warranted to unravel the role of uterine PMN viability and functionality in bovine uterine health.
Periodontal and Dental Status in Packs of Spanish Dogs
Authors: Whyte Ana , Whyte Jaime , Monteagudo Luis V. , García-Barrios Alberto , Tejedor M. Teresa
Published: April 2021

While periodontal disease (PD) is the most common canine oral pathology, its prevalence varies according to diagnosis methodology, breed, and age. We intended to increase understanding of canine PD by studying dogs that are managed in a specific way: pack dogs in Spain. They received a mixed diet (home-prepared food, commercial dry food, stale bread and bones). Thirty-two conscious individuals from two packs of dogs in Northeastern Spain (30/32 crossbred hunting dogs and 2/32 Siberian Husky; 26 males and 6 females; 27.75 ± 5.807 kgs; 5.48 ± 2.818 years) received visual dental examination for assessment of absent teeth (AT), dental calculus (DC) grade, gingival recession (GR), periodontal disease (PD), tooth fracture (TF), and dental attrition (DA). DC was the most prevalent oral problem (75%), followed by TF/DA (68.75%), AT (34.37%), GR (31.25%), and, finally, PD (15.62%). Low individual affectation values were found for AT, GR, and PD (<1 tooth/individual); mean DC grade per individual was 0.06 ± 0.063; and TF and DA were found in 1.63 and 4.72 teeth/individual, respectively. Low prevalence and extent of PD was attributed to diagnosis methodology, bodyweight effect, breed, and, ultimately, diet. Individuals affected by DC remained under veterinary surveillance due to PD development.
Voices of NGOs Supporting the First Master’s Degree Program in Ethology and Human-Animal Interactions in Romania: An Exploratory Qualitative Analysis
Authors: Rusu Alina Simona , Criste Adriana Dalila , Dezmirean Daniel Severus
Published: April 2021

This study aims to explore through a qualitative thematic analysis the reflections of six animal protection and wildlife conservation NGOs in Romania regarding the development of the first specialized master’s degree program in ethology and human-animal interaction (EHAI), in relation to the identified needs based on their experience in the field, as well as their expectations in terms of collaboration with the graduates of such a program in addressing the common problems in the areas of animal welfare and human-animal interactions (HAIs). The qualitative content analysis of the written reflections allowed the identification of two themes, which provided valuable insights regarding the curriculum offerings and the pedagogical strategies that could respond to the expressed needs and expectations. The first theme included seven categories of concerns expressed by the NGOs over the animal welfare and HAIs in Romania, i.e., concerns over stray animals, lack of education of general population on HAI and animal welfare, maltreatment of animals, human-animal conflicts, lack of professional specializations in HAI, concerns over common global issues, and concerns over national nature conservation. The second theme refers to the expectations regarding the roles of the graduates of the EHAI master’s program, and it includes four categories of codes: agents for change towards a responsible community, problem solvers, public policy-makers, and providers of competence-based expertise. The inclusion of Service-Learning as signature pedagogy in the EHAI program, in connection with the One Welfare approach, is discussed in relation to the needs expressed by the representatives of the NGOs.
Prevalence of Dichelobacter nodosus and Ovine Footrot in German Sheep Flocks
Authors: Storms Julia , Wirth Anna , Vasiliadis Danae , Brodard Isabelle , Hamann-Thölken Antje , Ambros Christina , Moog Udo , Jores Jörg , Kuhnert Peter , Distl Ottmar
Published: April 2021

The bacterium Dichelobacter nodosus (D. nodosus) is the causative agent of ovine footrot. The aim of this field study was to determine the prevalence of D. nodosus in German sheep flocks. The sheep owners participated voluntarily in the study. More than 9000 sheep from 207 flocks were screened for footrot scores using a Footrot Scoring System from 0 to 5 and sampling each sheep using one interdigital swab for all four feet of the sheep. The detection and discrimination between benign and virulent strains was done employing a real-time PCR. Our results showed a mean prevalence of 42.93% of D. nodosus in German sheep on an animal level. Underrunning of hoof horn on at least one foot (Scores 3-5) was detected in 567 sheep (6.13%). Sheep with four clinically healthy feet were found through visual inspection in 47.85% of all animals included in this study. In total, 1117 swabs from sheep with four clinically healthy feet tested positive for D. nodosus. In 90.35% of the positive swabs, virulent D. nodosus were detected. Benign D. nodosus were detected in 4.74% of the D. nodosus-positive swabs while 4.91% tested positive for both, benign and virulent D. nodosus. In 59 flocks D. nodosus were not detected and in 115 flocks only virulent D. nodosus were found while seven flocks tested positive for benign strains.
Pork Quality of Two Lithuanian Breeds: Effects of Breed, Gender and Feeding Regimen
Authors: Razmaitė Violeta , Juška Remigijus , Leikus Raimondas , Jatkauskienė Virginija
Published: April 2021

The diversity of breeds is an important factor influencing carcass and meat quality traits that are also associated with other different effects. The objective of this study was to investigate the effects of breed, gender, and feeding level in the finishing phase on the carcass and meat quality of Lithuanian White and Lithuanian Indigenous Wattle pigs reared indoors. After 60 kg weight, half of the animals from both breeds were fed a restricted diet of approximately 82% of average ad libitum feeding intake, and the other half of pigs were further fed ad libitum to the end of the experiment. Feed restriction during the finishing phase decreased daily gain and weight of pigs at slaughter, and backfat thickness at the tenth rib (p ˂ 0.001, p ˂ 0.01, and p ˂ 0.05, respectively). Lithuanian White pigs demonstrated higher (p ˂ 0.001) growth rate, live body weight at slaughter and carcass weight, and had a higher (p ˂ 0.01) length of carcass and loin area and lower (p ˂ 0.05) backfat thickness at the last rib and at two points at the lumbar area compared with Lithuanian Indigenous Wattle pigs. The semimembranosus muscle of Lithuanian White pigs had relatively (8.1%) lower (p ˂ 0.001) proportions of saturated and 41.2% higher (p ˂ 0.001) proportions of polyunsaturated fatty acids compared with the pigs of the Lithuanian Indigenous Wattle breed, whereas in the longissimus muscle and subcutaneous tissue, the breed only affected the n-6/n-3 ratio (p ˂ 0.01 and p ˂ 0.001, respectively). Gender showed an effect on saturated fatty acids in all the studied tissues, however, the effects on polyunsaturated fatty acids were found in the semimembranosus muscle and subcutaneous tissue (p ˂ 0.05 and p ˂ 0.001) and the effects on monounsaturated fatty acids was found only in the semimembranosus muscle (p ˂ 0.01). These differences in the fatty acid composition of the semimembranosus muscle and subcutaneous tissue of females exhibited more favorable lipid quality indices compared with castrated males (p ˂ 0.001). The semimembranosus muscle of Lithuanian White pigs also showed more favorable lipid quality indices in relation to healthy nutrition compared with Lithuanian Indigenous Wattle pigs (p ˂ 0.001). This study is one of the steps toward the development and utilization of endangered breeds. The obtained information can be used to increase choice in pork production and consumption, and provides new insights for research into the conservation of local breeds.
PK/PD Analysis of Marbofloxacin by Monte Carlo Simulation against Mycoplasmaagalactiae in Plasma and Milk of Lactating Goats after IV, SC and SC-Long Acting Formulations Administration
Authors: Fernández-Varón Emilio , García-Romero Edgar , Serrano-Rodríguez Juan M. , Cárceles Carlos M. , García-Galán Ana , Cárceles-García Carlos , Fernández Rocío , Muñoz Cristina , de la Fe Christian
Published: April 2021

Contagious agalactia is a mycoplasmosis affecting small ruminants that have become an important issue in many countries. However, PK/PD studies of antibiotics to treat this problem in lactating goats affected by Mycoplasma (M.) agalactiae, the main CA-causing mycoplasma are almost non-existent. The aims of this study were to evaluate the plasma and milk disposition of marbofloxacin in lactating goats after intravenous (IV), subcutaneous (SC) and subcutaneous poloxamer P407 formulations with and without carboxy-methylcellulose (SC-P407-CMC and SC-P407) administration. Marbofloxacin concentrations were analysed by the High Performance Liquid Chromatography (HPLC) method. Minimum inhibitory concentrations (MIC) of M. agalactiae field isolates from mastitic goat’s milk were used to calculate surrogate markers of efficacy. Terminal half-lives of marbofloxacin after IV, SC, SC-P407 and SC-P407-CMC administration were 7.12, 6.57, 13.92 and 12.19 h in plasma, and the half-lives of elimination of marbofloxacin in milk were 7.22, 7.16, 9.30 and 7.74 h after IV, SC, SC-P407 and SC-P407-CMC administration, respectively. Marbofloxacin penetration from the blood into the milk was extensive, with Area Under the Curve (AUCmilk/AUCplasma) ratios ranged 1.04–1.23, and maximum concentrations (Cmax-milk/Cmax-plasma) ratios ranged 0.72–1.20. The PK/PD surrogate markers of efficacy fAUC24/MIC and the Monte Carlo simulation show that marbofloxacin ratio (fAUC24/MIC > 125) using a 90% of target attainment rate (TAR) need a dose regimen between 8.4 mg/kg (SC) and 11.57 mg/kg (P407CMC) and should be adequate to treat contagious agalactia in lactating goats.
Mechanisms of Antibiotic Resistance in Important Gram-Positive and Gram-Negative Pathogens and Novel Antibiotic Solutions
Authors: Kakoullis Loukas , Papachristodoulou Eleni , Chra Paraskevi , Panos George
Published: April 2021

Multidrug-resistant bacteria have on overwhelming impact on human health, as they cause over 670,000 infections and 33,000 deaths annually in the European Union alone. Of these, the vast majority of infections and deaths are caused by only a handful of species—multi-drug resistant Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus spp., Acinetobacter spp. and Klebsiella pneumoniae. These pathogens employ a multitude of antibiotic resistance mechanisms, such as the production of antibiotic deactivating enzymes, changes in antibiotic targets, or a reduction of intracellular antibiotic concentration, which render them insusceptible to multiple antibiotics. The purpose of this review is to summarize in a clinical manner the resistance mechanisms of each of these 6 pathogens, as well as the mechanisms of recently developed antibiotics designed to overcome them. Through a basic understanding of the mechanisms of antibiotic resistance, the clinician can better comprehend and predict resistance patterns even to antibiotics not reported on the antibiogram and can subsequently select the most appropriate antibiotic for the pathogen in question.
Potential Anticancer Lipoxygenase Inhibitors from the Red Sea-Derived Brown Algae Sargassum cinereum: An In-Silico-Supported In-Vitro Study
Authors: Alzarea Sami I. , Elmaidomy Abeer H. , Saber Hani , Musa Arafa , Al-Sanea Mohammad M. , Mostafa Ehab M. , Hendawy Omnia Magdy , Youssif Khayrya A. , Alanazi Abdullah S. , Alharbi Metab , Sayed Ahmed M. , Abdelmohsen Usama Ramadan
Published: April 2021

LC-MS-assisted metabolomic profiling of the Red Sea-derived brown algae Sargassum cinereum “Sargassaceae” dereplicated eleven compounds 1–11. Further phytochemical investigation afforded two new aryl cresol 12–13, along with eight known compounds 14–21. Both new metabolites, along with 19, showed moderate in vitro antiproliferative activity against HepG2, MCF-7, and Caco-2. Pharmacophore-based virtual screening suggested both 5-LOX and 15-LOX as the most probable target linked to their observed antiproliferative activity. The in vitro enzyme assays revealed 12 and 13 were able to inhibit 5-LOX more preferentially than 15-LOX, while 19 showed a convergent inhibitory activity toward both enzymes. Further in-depth in silico investigation revealed the molecular interactions inside both enzymes’ active sites and explained the varying inhibitory activity for 12 and 13 toward 5-LOX and 15-LOX.
Leaves and Fruits Preparations of Pistacia lentiscus L.: A Review on the Ethnopharmacological Uses and Implications in Inflammation and Infection
Authors: Milia Egle , Bullitta Simonetta Maria , Mastandrea Giorgio , Szotáková Barbora , Schoubben Aurélie , Langhansová Lenka , Quartu Marina , Bortone Antonella , Eick Sigrun
Published: April 2021

There is an increasing interest in revisiting plants for drug discovery, proving scientifically their role as remedies. The aim of this review was to give an overview of the ethnopharmacological uses of Pistacia lentiscus L. (PlL) leaves and fruits, expanding the search for the scientific discovery of their chemistry, anti-inflammatory, antioxidative and antimicrobial activities. PlL is a wild-growing shrub rich in terpenoids and polyphenols, the oil and extracts of which have been widely used against inflammation and infections, and as wound healing agents. The more recurrent components in PlL essential oil (EO) are represented by α-pinene, terpinene, caryophyllene, limonene and myrcene, with high variability in concentration depending on the Mediterranean country. The anti-inflammatory activity of the oil mainly occurs due to the inhibition of pro-inflammatory cytokines and the arachidonic acid cascade. Interestingly, the capacity against COX-2 and LOX indicates PlL EO as a dual inhibitory compound. The high content of polyphenols enriching the extracts provide explanations for the known biological properties of the plant. The protective effect against reactive oxygen species is of wide interest. In particular, their anthocyanins content greatly clarifies their antioxidative capacity. Further, the antimicrobial activity of PlL oil and extracts includes the inhibition of Staphylococcus aureus, Escherichia coli, periodontal bacteria and Candida spp. In conclusion, the relevant scientific properties indicate PlL as a nutraceutical and also as a therapeutic agent against a wide range of diseases based on inflammation and infections.
Antimicrobial Use in Animals in Timor-Leste Based on Veterinary Antimicrobial Imports between 2016 and 2019
Authors: Ting Shawn , Pereira Abrao , Alves Amalia de Jesus , Fernandes Salvador , Soares Cristina da Costa , Soares Felix Joanico , Henrique Onofre da Costa , Davis Steven , Yan Jennifer , Francis Joshua R. , Barnes Tamsin S. , Jong Joanita Bendita da Costa
Published: April 2021

Monitoring veterinary antimicrobial use is part of the global strategy to tackle antimicrobial resistance. The purpose of this study was to quantify veterinary antimicrobials imported into Timor-Leste between 2016 and 2019 and describe the antimicrobial import profile of importers. Data were obtained from import applications received by the Ministry of Agriculture and Fisheries (MAF) of Timor-Leste. Import quantities were analysed by antimicrobial class, importance for human medicine, recommended route of administration and type of importer. An average of 57.4 kg (s.d. 31.0 kg) and 0.55 mg/kg (s.d. 0.27 mg/kg) animal biomass of antimicrobials was imported per year. Tetracyclines (35.5%), penicillins (23.7%), and macrolides (15.9%) were the commonly imported antimicrobial classes. Antimicrobials imported for parenteral administration were most common (60.1%). MAF was the largest importer (52.4%). Most of the critically important antimicrobials for human medicine were imported by poultry farms for oral administration and use for growth promotion could not be ruled out. In conclusion, the use of antimicrobials in animals in Timor-Leste is very low, in keeping with its predominantly subsistence agriculture system. Farmer education, development of treatment guidelines, and strengthening of the veterinary service is important for addressing the potential future misuse of antimicrobials especially in the commercial poultry industry.
New Amides and Phosphoramidates Containing Selenium: Studies on Their Cytotoxicity and Antioxidant Activities in Breast Cancer
Authors: Etxebeste-Mitxeltorena Mikel , Plano Daniel , Astrain-Redín Nora , Morán-Serradilla Cristina , Aydillo Carlos , Encío Ignacio , Moreno Esther , Espuelas Socorro , Sanmartín Carmen
Published: April 2021

Breast cancer is a multifactor disease, and many drug combination therapies are applied for its treatment. Selenium derivatives represent a promising potential anti-breast cancer treatment. This study reports the cytotoxic activity of forty-one amides and phosphoramidates containing selenium against five cancer cell lines (MCF-7, CCRF-CEM, HT-29, HTB-54 and PC-3) and two nonmalignant cell lines (184B5 and BEAS-2B). MCF-7 cells were the most sensitive and the selenoamides I.1f and I.2f and the selenium phosphoramidate II.2d, with GI50 values ranging from 0.08 to 0.93 µM, were chosen for further studies. Additionally, radical scavenging activity for all the compounds was determined using DPPH and ABTS colorimetric assays. Phosphoramidates turned out to be inactive as radical scavengers. No correlation was observed for the antioxidant activity and the cytotoxic effect, except for compounds I.1e and I.2f, which showed dual antioxidant and antitumor activity. The type of programmed cell death and cell cycle arrest were determined, and the results provided evidence that I.1f and I.2f induced cell death via autophagy, while the derivative II.2d provoked apoptosis. In addition, Western blot analysis corroborated these mechanisms with an increase in Beclin1 and LC3-IIB and reduced SQSTM1/p62 levels for I.1f and I.2f, as well as an increase in BAX, p21 and p53 accompanied by a decrease in BCL-2 levels for derivative II.2d.