bluuebunny/crossref_metadata_2025 huggingface dataset:
Reflectometric Analysis for Identification of Various Pathological Conditions Associated with Lichen Planus
Authors: Georgescu Simona Roxana , Ene Corina Daniela , Nicolae Ilinca , Mitran Madalina , Musetescu Alina , Matei Clara , Rusu Laura Cristina , Tampa Mircea
Published: February 2020

Reflectometric technique for analyzing urine strips represents a simple, fast and inexpensive method, with no risk and discomfort for the patient. In the present paper, the authors conducted a retrospective study on the possible association between lichen planus (LP) and a number of pathological conditions that produce changes of urine metabolites. A retrospective study was made on 77 patients with LP (49 cases of cutaneous LP - CLP, 28 cases of oral LP - OLP) who were diagnosed and treated in the dermatology department of Victor Babes Hospital for Infectious and Tropical Diseases. The quantification of certain urine metabolites in the spontaneous urine through reflectometric technique may reveal liver diseases (urobilinogen, bilirubin), kidney diseases (erythrocytes, proteins, albumin, creatinine, albumin/creatinine ratio, urine specific gravity, pH), metabolic disorders (glucose, ketones, pH, ascorbic acid) and urinary diseases (leucocyturia, nitrites, proteins, erythrocytes). The evaluation of the patients included clinical examination and laboratory and imaging tests. Among the investigated patients with LP we found: 16 cases of hepatic dysfunction (20.8%), 7 cases of renal dysfunction (9.1%), 11 cases of metabolic disorders (14.3%) and 14 cases of urinary tract infections (18.2%). No differences were found between patients with cutaneous and oral LP. The linear regression analysis revealed: positive association between concentrations of urobilinogen and bilirubin (r = 0.174, p = 0.044), negative association between albumin and creatinine (r = -0.316, p = 0.007), positive association between glucose and ketones (r = 0.266, p = 0.098) and positive association between leucocyturia and urine nitrite levels (r = 0.202, p = 0.050). Based on clinical and laboratory data, 85.7% of results obtained from urine biochemistry were confirmed. This analysis did not find any difference between patients with cutaneous and oral LP. By determining a full panel of urine metabolites through reflectometric analysis we were able to identify the main pathological changes in patients with LP.
Nickel-Titanium Closed-coil Springs. Evaluation of the clinical plateau
Authors: Bezrouk Ales , Balsky Libor , Krulichova Iva Selke , Smutny Martin , Zahora Jiri , Selke Gisbert W. , Meling Torstein R.
Published: February 2020

In this in vitro study, we tested 10 types of springs from 5 manufacturers. We performed a simulation of the clinically relevant action of the spring during its application and treatment of the patient. For determining the deactivation plateau, we developed an innovative statistical method, making it feasible to precisely find and evaluate the necessary clinically relevant parameters of all types of NiTi SE closed-coil springs in the market. In terms of the application simplicity, the 3M 12 and 3M 9 springs proved to be the most suitable.
A Comparative Study of Metal Content in Selected Polish and Romanian Honey Samples
Authors: Purcarea Cornelia , Dzugan Malgorzata , Wesolowska Monika , Chis Adriana Monica , Zagula Grzegorz , Teusdea Alin Cristian , Puchalski Czeslaw
Published: February 2020

The aim of the study was to compare the heavy metal contamination and study the mineral content of Polish and Romanian honeys. We used Polish varietal honeys (n=18) produced in the year 2013-2014 in ecologically clean Podkarpackie region, and Romanian honeys (n=36) from Bihor district produced in 2013. The heavy metals concentration as well as mineral composition of honey was assayed by ICP-OES method with prior microwave mineralization. The cadmium and lead level in tested Polish honeys ranged from 0.007 to 0.021mg/kg and 0.02 to 0.098 mg/kg, respectively. Cadmium was not detected in Romanian honeys, and the lead contamination was lower than in Polish honey (0.018-0.05 mg/kg). Mercury was not detected in any tested honey samples. The aluminum content in Polish honeydew honeys was 34.6-times higher than in Romanian honeys. Honey samples from Poland were richest in potassium (2.5-fold for honeydew, lime and acacia, excluding heather), but they included less calcium. Generally, dark honeys include the highest mineral concentration and are more contaminated by heavy metals. However, all tested samples met the legal requirements for heavy metals residues. The geographical origin and soil composition strongly influenced honey chemical composition and the crucial factor for heavy metal transfer seems to be soil pH.
Total Polyphenol Content and Antiproliferative Activity of Green Tea Extracts Collected from Romanian Pharmaceuticals Market
Authors: Danciu Corina , Alexa Ersilia , Avram Stefana , Minda Daliana , Pavel Ioana Zinuca , Babuta Roxana , Mioc Marius , Ledeti Ionut , Cuc Hepcal Ioana , Motoc Andrei
Published: February 2020

Total polyphenol content and anti proliferative activity of five green tea extracts available on the Romanian pharmaceuticals market was analyzed. Results show dose-dependent anti proliferative activity of the selected samples against B164A5 mouse melanoma and A375 human melanoma cell lines. Sample 3, the richest sample in polyphenols showed the highest anti proliferative potential against the screened melanoma cell lines.
Methylene Blue Staining Test in Early Detection of Head and Neck Cancers
Authors: Stefan Catalin , Lostun Gabriel , Lostun Alexandra
Published: February 2020

Head and neck cancers represent an important health problem in the present time. Due to the often late diagnosis and the significant changes that accompany the therapy associated with this disease, the impact on the quality of life of the patient is remarkable, causing a lower adherence to treatment, poor outcomes and an unfavorable prognosis. The current trend focuses on methods of investigation that allow an early diagnosis, which in turn translates into minimally invasive interventions with a favorable outcome. The purpose of this paper is to present the methylene blue staining method as an diagnosis tool, allowing the surgeon a better evaluation of the tumor and a more reliable limit of resection.
Applicability of Ferromagnetic Nanoparticles in the Retention of Heavy Metals from Aqueous Solutions
Authors: Clej (Slave) Dumitra Daniela , Ardelean Ioana Lavinia , Nechifor Gheorghe
Published: February 2020

In this paper are presented the experimental results obtained in the study of applicability of ferromagnetic nanoparticles in the retention of heavy metals from aqueous solutions. The influence of magnetic nanoparticles based on iron, cobalt and nickel on turbidity, pH, permanganate index, aluminum, copper, cadmium and lead was studied. The study shows that conventional water treatment systems combined with acrylic polymer can be improved by adding magnetic nanoparticles. Experimental data has shown that the presence of magnetic nanoparticles based on iron, cobalt and/or nickel increases the efficiency of the treatment system and is reflected by all analyzed parameters.
Oral and Maxillofacial Manifestations of Mineral and Bone Disorders Associated with Chronic Renal Failure
Authors: Doscas Andrada Raluca , Balan Mihail , Ciofu Mihai Liviu , Agop Forna Doriana , Martu Marius Cristian , Popescu Eugenia
Published: February 2020

Chronic kidney disease (CKD) is a multifactorial syndrome and a global health concern. As renal function declines, there is a progressive deterioration of mineral homeostasis. Starting from stage 3 of CKD oral manifestations of mineral disorders can occasionally appear and become more frequent and evident in stage 5. We retrospectively analysed 43 patients diagnosed with end stage renal failure undergoing dialysis, hospitalized in our clinic for different oral and maxillofacial pathologies. The mean dialysis period was 5.43 years. Radiographic alterations afecting the jaws were found in all patients. The most common feature was partial or total loss of lamina dura, followed by alterations of the bony trabeculae. 9 patients presented brown tumors which are considered the final stage of secondary hyperparathyroidism associated with renal failure.
The Influence of the Curing Temperature on the Properties of Some Silane Films
Authors: Ciobotaru Ioana Alina , Vaireanu Danut Ionel , Ciobotaru Irina Elena , Ciobotea Barb Oana Claudia
Published: February 2020

This paper discusses the importance of the temperature drying process on some silane films in order to obtain a coating with specific properties (stability in time, resistance to corrosion attack and compactness) that would make them appropriate to be used as anticorrosive protection layers. Electrochemical techniques such as open circuit potential and electrochemical impedance spectroscopy were employed in order to characterize the investigated deposited silane films on aluminium substrate. The validation of the experimental electrochemical impedance spectroscopy data was carried out using the comparison of the validation coefficient versus the Pearson coefficient computed for a a=0.05.
Purity Determination of Fusidic Acid Using Physico-chemical Methods
Authors: Marian Eleonora , Duteanu Narcis , Vicas Laura , Tita Bogdan , Sfirloaga Paula , Jurca Tunde
Published: February 2020

When a new drug product is prefomulated and later is accepted as a future commercial product is important to test stability and also higher purity of medical substances which should be processed during drug production.
In present paper was checked the purity of fusidic acid, a relatively new antibiotic acting on gram positive bacteria, and is used as treatment for acne vulgaris. Purity and also thermal stability of this new class of antibiotics was tested using physicochemical test methods, such as: Fourier transform Infrared Spectroscopy (FTIR), XRay Diffraction (XRD), Scanning Electron Microscopy (SEM), and also Thermal Analysis.
Use of Phosphatidylcholine in the Treatment of Localized Fat Deposits. Results and expectations
Authors: Berbece Sorin , Iliescu Dan , Ardeleanu Valeriu , Nicolau Alexandru , Jecan Radu Cristian
Published: February 2020

Obesity represents a global health problem. According to the latest studies released by the World Health Organisation (WHO), 1.7 billion currently in excess of normal weight individuals, of which approx. 75% are overweight (body mass index - BMI 25 to 30). The common form of excess adipose tissue manifestation in overweight individuals is localized fat deposits with high (abdominal) or low (buttocks and thighs) disposition. Although the overweight can be corrected relatively easy by changing behavioral habits or food, a constant physical exercises program or following a diet food are not accessible to all through the efforts of will, financial and time involved. Several methods have been studied and tested over time to eliminate more or less invasive fat deposits with varying efficacy and adverse effects. Chemical lipolysis using phosphatidylcholine as the basic substance was initially used in hypercholesterolemia and its complications and was rapidly adopted in mesotherapy techniques for the treatment of fat deposits. This study reveals the results obtained using Dermastabilon on a sample of 16 patients, the time allocated to treatment and discomfort being minimal, and rapid and notable results. There were no side effects.
Heart-Type Fatty Acid-Binding Protein (H-FABP) in Patients with Coronary Artery Bypass Graft Surgery Undergoing Cardiac Rehabilitation Program
Authors: Namat Razan Al , Aursulesei Viviana , Felea Maura Gabriela , Costache Irina Iuliana , Petris Antoniu , Mitu Ovidiu , Namat Nadia Al , Constantin Mihai , Namat Dina Al , Ghiciuc Cristina , Lupusoru Catalina Elena , Tinica Grigore , Mitu Florin
Published: February 2020

Heart-type Fatty Acid-Binding Protein (H-FABP), compared with classical biomarkers, proved to have high sensitivity for myocardial damage size in patients undergoing cardiac surgery. High H-FABP levels are strongly associated in case of death, post-operatively acute kidney injury and atrial fibrillation. Cardiac rehabilitation is an instrument of medical management in cardiovascular diseases; beyond prevention, it can improve heart and muscle functioning in patients that were undergoing CABG, and cardiac and vascular adaptation. Over a 2-year period, 110 subjects were randomized and comprehensively evaluated. The mean age of the patients under study was 65.70 � 9.91 years old. For the H-FABP, the mean value in the Phase I was 67.40 � 9.81 ng/mL, while the mean value in Phase III was 4.80 � 2.30 ng/mL. The difference registered between the plasma H-FABP value in the first 24 h after cardiac surgery and the value in 6 months after the onset of cardiac rehabilitation program was important and statistically significant, as p [ 0.05. Fibrinogen showed significant phase-to-phase reductions of plasmatic values. Lipid profile values showed a statistically significant decrease. The renal filtration function evaluated by plasma creatinine showed statistically significant improvement and, in terms of absolute values, creatinine level was reduced in a range between 0.2-0.4 mg/dL. Also, it was recorded a significantly lower level of blood urea. The reduction of plasma H-FABP values were registered between the first phase (the first 24 h) after cardiac surgery and the third phase of the cardiac rehabilitation program. H-FABP protein had a higher sensitivity and specificity when compared to other enzymes of myocardial cytolysis.
Dimethyldisulphide Hydrodesulphurization on NiCoMo / Al2O3 Catalyst
Authors: Doukeh Rami , Bombos Mihaela , Trifoi Ancuta , Pasare Minodora , Banu Ionut , Bolocan Ion
Published: February 2020

Hydrodesulphurization of dimethyldisulphide was performed on Ni-Co-Mo /�-Al2O3 catalyst. The catalyst was characterized by determining the adsorption isotherms, the pore size distribution and the acid strength. Experiments were carried out on a laboratory echipament in continuous system using a fixed bed catalytic reactor at 50-100�C, pressure from 10 barr to 50 barr, the liquid hourly space velocity from 1h-1 to 4h-1 and the molar ratio H2 / dimethyldisulphide 60/1. A simplified kinetic model based on the Langmuir�Hinshelwood theory, for the dimethyldisulphide hydrodesulfurization process of dimethyldisulphide has been proposed. The results show the good accuracy of the model.
Study Regarding the Presence of Zn(II) in Thermal Waters for Cosmetic Use
Authors: Andoni Mihaiela , Savoiu Balint Germaine , Simu Georgeta Maria , Popovici Ramona Amina , Stanca Horia Tudor
Published: February 2020

The experimental investigations aimed to determine the optimal conditions for the process of zinc removal from thermal waters with the PUROLITE S920 ion exchange resin. It was determined the residual zinc concentration dependence on the amount of ion exchanger at different initial zinc concentrations in solution and different stirring times. The optimum conditions for achieving a residual concentration below 1 mg/L are: ion exchange rate 1.6 g/L, stirring time about 45 min. The PUROLITE S920 ion exchanger can be successfully used to remove very small amounts of zinc ions from the solution.
Selenium- Essential Antioxidant Element. The example of autoimune thyroiditis
Authors: Preda Cristina , Vasiliu Ioana , Mihalache Laura , Armasu Ioana , Serban Ionela Lacramioara , Serban Dragomir Nicolae , Stoica Bogdan , Ciobanu Delia Gabriela , Bredetean Ovidiu , Strungaru Stefan Adrian , Nicoara Mircea , Plavan Gabriel , Vulpoi Carmen
Published: February 2020

To evaluate the effect of Selenium (Se) supplementation on: thyroid stimulating hormone (TSH), antiperoxidase antibodies (TPOAb) and glutathione peroxidise 1 (GPx1) in euthyroid subjects with autoimmune thyroiditis. 100 euthyroid women with autoimmune thyroiditis, from the same region, were randomized to receive daily 100 mg selenomethionine (n=50) or placebo (n=50) for 3 months. Serum concentrations of Se, TPOAb and TSH were performed in all patients at baseline and after 3 months. GPx1 activity was measured only in the interventional group before and after Se supplementation. At 3 months TSH presented a significant increase both in treated (2.49 vs. 2.09 UI/mL; p=0.001) and untreated groups (2.38 vs. 1.91 UI/mL; p=0.008). TPOAb decreased by 15.2% in patients treated with Se (p=0.002) and were not modified in untreated patients. At the end of the study Se and TPOAb were in direct insignificant correlation (r=+0.267, p=0.105). GPx1 did not show significant changes after Se supplementation. After 3 months of Se supplementation results showed a mild decrease of TPOAb and a weak negative correlation of these antibodies with Se levels. This suggests that Se treatment may improve the course of thyroid autoimmunity.
Distribution of Biochemical and Ultrasound Markers Values in the First Trimester Screening Program in Timisoara
Authors: Navolan Dan , Vladareanu Simona , Ciohat Ioana , Carabineanu Adrian , Craina Marius , Nemescu Dragos , Birsasteanu Bogdan , Onofriescu Alina , Boia Marioara , Tepetzikiotis Efstathios , Craciunescu Mihaela , Birsasteanu Florin
Published: February 2020

The aim of our study was to analyze the distribution of biochemical markers (free-� hCG and PAPP-A) and nuchal translucency (NT) values and their deviation from expected median in our screening program. NT, free-� hCG and PAPP-A values were measured in first trimester sera of 1313 singleton pregnant women. The age (mean �standard deviation) of pregnant women at the time of screening was 28.94�4.95 years and the gestational age was 87.66�4.74 days. The value of NT (median�standard error of mean) was 0.87�0.01 multiple of median corrected (MoMc), of free-b hCG was 1.07�0.02 MoMc and of PAPP-A was 1.06�0.01 MoMc. Since the huge majority of pregnant women carried a healthy fetus it was assumed that the median value of measurements was equal to 1 MoMc and the deviation from median was equal to null. Our results showed that the greatest deviation from the expected median (-0.13 MoMc) was recorded for the NT measurement while the deviation for the free-� hCG and PAPP-A values was 0.07 respectively 0.06. In conclusion the main disturbing factor in calculating the aneuploidy risk was the measurement of NT. A systematic audit of laboratory and ultrasound measurement is necessary.
Simultaneous Determination of alfa-, beta- and gama-hexabromocyclododecane Diastereoisomers in Sewage Sludge using Liquid Chromatography Tandem Mass Spectrometry
Authors: Chiriac Florentina Laura , Cruceru Liliana , Niculescu Marcela , Pascu Luoana Florentina , Blaziu Lehr Carol , Galaon Toma
Published: February 2020

Hexabromocyclododecane (HBCD) is a large-scale usage brominated flame retardant consisting of a mixture of diastereoisomers which has been reported as an ubiquitous environmental contaminant. HBCD has attracted much attention due to its toxicity and increasing levels in waste water sewage sludge. Since November 2014, HBCD was added to the Persistent Organic Pollutants list of the Stockholm Convention which generated a higher concern regarding its presence in the environment. A rapid (8 min) and accurate liquid chromatography electrospray ionization tandem mass spectrometry method (LC-ESI-MS/MS) was developed to separate and detect a-, b- and g-HBCD isomers from sewage sludge. Optimization of LC-MS parameters (column temperature, mobile phase composition and flow, collision energy, fragmentor voltage, capillary voltage and drying gas temperature) allowed complete separation of the three isomers and also very good detection sensitivity with instrumental quantitation limits (IQLs) between 0.3 and 0.6 ng/mL. The three isomers were separated on a C18 reversed-phase column, kept at 18�C, using a H2O/ACN/MeOH mobile phase mixture and detected by negative electrospray ionization using MRM mode with two transitions, one for quantitation and one for confirmation. MS detector response was linear in the range 1.0 � 100.0 ng/mL with correlation coefficients (R2) higher than 0.99 for all three isomers. A simple ultrasonic assisted liquid-solid extraction procedure using a solvent mixture was employed to extract HBCD isomers from WWTP sewage sludge. Overall method intra-day and inter-day precision (RSD%) were situated between 7.6 � 9.2% and 10.3 � 14.5%, respectively. LOQs for a-, b- and g-HBCD were 1.4, 0.7 and 1.0 ng/g, comparable and even lower to those reported by similar studies concerning HBCD presence in WWTP sewage sludge. HBCD presence was tested from several WWTP sludge samples. All three isomers were found with 100% detection frequency ranging from 3 to 76 ng/g.
Comparing Carbonyls Levels in Indoor Air in two Offices -Green and Old Building
Authors: Diodiu Raluca , Galaon Toma
Published: February 2020

The concentrations of 17 carbonyl compounds were investigated in indoor air of two offices - one located in a green building and the other in an old building, both from Bucharest. Indoor air samples were collected during normal activities of the working staff on passive samplers. Two analytical techniques were used: Ion Chromatography for carboxylic acids and High Performance Liquid Chromatography for aldehydes and ketones. A questionnaire was administrated in order to collect information about buildings characteristics, indoor furniture, decorating materials and electronical devices used during the sampling. Formaldehyde, acroleine+acetone, acetaldehyde, hexaldehyde and carboxylic acids were the most abundant compounds that accounted for more than 50% of the total carbonyls. The majority of the aldehydes showed higher concentrations in the old building office compared to the concentrations found in the green building office. Regarding the carboxylic acids: the concentration of formic acid was slightly higher in the old building office versus the green building office; in the case of acetic acid, the concentration found in the old building office was around two times higher comparing to the green building office.
Metallic Elements (Cu, Zn, Ni and Mn) Toxicity Effects Determination on a Fresh Water Fish Cyprinus Carpio (Common Carp) Laboratory Acclimatized
Authors: Gheorghe Stefania , Vasile Gabriela Geanina , Gligor Cristina , Lucaciu Irina Eugenia , Lazar Mihai Nita
Published: February 2020

Metallic elements copper (Cu), zinc (Zn), nickel (Ni) and manganese (Mn) are some of the most commonly found in water and sediment samples collected from the Danube - Danube Delta. These elements are important as essential micronutrients, being normally present at low concentrations in biological organisms, but in high concentrations they become toxic with immediate and delayed effects. The role of this metals is still controversial, that�s why bioconcentration potential is so important. In this non-clinical study, we tested in vitro effect of heavy metals on carp, Cyprinus carpio, reproducing in vivo presence of Cu, Zn, Ni and Mn in the Romanian�s surface water. The toxicity tests were performed according to OECD 203 by detecting the average (50%) lethal concentration - LC50 on aquatic organisms (freshwater fish) at 96h. The results pointed out that, copper value for LC 50 at 96h was estimated as 3.4 mg/L (concentrations tested in the range of 0.1 - 4.75 mg/L). Zinc value for LC 50 at 96h was estimated as 20.8 mg/L (concentrations tested in the range of 0.028 � 29.6 mg/L). Nickel value for LC 50 at 96h was estimated as 40.1 mg/L (concentrations tested in the range of 0.008 - 84.5 mg/L). For manganese the mortality effects has recorded at LC 50 at 96h at estimated value higher than 53 mg/L (concentrations tested in the range of 0.04 - 53.9 mg/L). The accuracy of the testing metals concentration was insured by the screening of the dilution water, as well as food and control fish, acclimated in laboratory conditions.
Neonicotinoids Detection by new LC-MS/MS Method in Romanian Surface Waters
Authors: Iancu Vasile Ion , Galaon Toma , Niculescu Marcela , Blaziu Lehr Carol
Published: February 2020

Increasing and widespread use of neonicotinoid insecticides in all world, together with their highly toxicity to invertebrates and environmental persistence mean that surface waters need to be monitored for these compounds. In the 2015, neonicotinoid insecticides have been incorporated in the watch list of substances for a European Union monitoring program (495/2015/ EU). A new method using automated solid phase extraction (SPE) with polymeric cartridges (OASIS HLB) followed by LC-MS/MS provided good separation of the most common neonicotindoid compounds. The method was developed for the determination of four neonicotinoid insecticides (nitenpyram, thiamethoxam, clothianidin, acetamiprid) in surface water with low limit of quantification (0.3-0.9 ng/L, nanograms per liter). Recoveries in surface water samples fortified at 200 ng/L for each compound ranged from 71.4 to 109.9 %; relative standard deviation ranged from 4 to 9%. The method was applied to water samples from four streams in Romania, Danube River and its tributaries (Arges River, Jiu River, and Olt River). The surface water samples were found to be contaminated clothianidin (1.08-6.4 ng/L) and by thiamethoxam (1.1-3.8ng/L). The highest concentrations were recorded in Danube River in Oltenita point (6.4ng/L) and in Gura-Vaii point (5.5ng/L). The concentration of acetamiprid and nitenpyram were situated below limit of quantification in all samples.
Quantitative Analysis and Antibacterial Activity of Some Coumarins Extracts
Authors: Stanciu Gabriela , Aonofriesei Florin , Cristache Nicoleta , Lupsor Simona
Published: February 2020

Extracts of melilot, cinnamon and ash were analyzed with TLC and molecular absorption spectrometry. With TLC method coumarin and coumarins derivatives (fraxetin, psoralen and scopoletin) contained in the studied plants were identified. The coumarin concentrations in melilot (637.84 mg/100g d.w) and cinnamon (58.594 mg/100g d.w.) were the highest. Melilot and cinnamon extracts have been tested against two reference bacterial strains and against two clinical bacterial strains in order to preliminary evaluate their antimicrobial profile. Our results showed a significant response of bacterial growth on culture media when exposed to coumarin derivatives found in melilot and cinnamon extracts. Overall, the inhibitory activity of extracts was higher on Gram positive bacteria on one hand and on clinical strains on the other hand suggesting an important practical aspect.
Implications of Creatinine and ALP in the Induction of Osteoporosis by Ovariectomy in Female Wistar Rats
Authors: Lazar Adela Cristina , Lacatus Radu , Purdoiu Robert , Bel Lucia , Sarpataki Orsolya , Papuc Ionel , Pacurar Mariana , Campian Radu Septimiu
Published: February 2020

Histological and biochemical assessment of maxillary bone necrosis induced by therapy with two bisphosphonate types used to treat osteoporosis induced in female Wistar rats, evaluation of effects on creatinine and ALP values at different time intervals. Female Wistar rats were ovariectomized to induce osteoporosis. Blood tests for ALP and creatinine were conducted. They received two types of bisphosphonates. Dental extraction was performed in each rat separately; finally, the rats were euthanized. Microscopic evaluation of the tissue fragments collected from the groups of rats was performed for the following parameters: degree of osteonecrosis, bone resorption, inflammatory infiltrate with neutrophils and mononuclear cells, number of osteoclasts, degree of proliferation of fibrous connective tissue, and local neovascularization. The biochemical and subsequently histological investigations for osteonecrosis of the jaw associated with bisphosphonate therapy in rats with induced osteoporosis provide accurate information about the extent of osteonecrosis, bone resorption, inflammatory infiltrate with mononuclear cells and neutrophils, number of osteoclasts, degree of proliferation of fibrous connective tissue, and local neovascularization. This is necessary for positive and differential diagnosis and for the treatment plan.
Link Between Biochemical, Biological and Clinical Assessment Focused on Polycythemia Vera and Stroke
Authors: Nica Adriana Sarah , Nartea Roxana , Stefan Daciana Andrada Costina , Miclaus Roxana
Published: February 2020

This case presentation is of a 58 years old female patient who develops a stroke in 2011 with significant biochemical and biological changes. These manifestations prove to be the result of a hematology disorder, more precise a myeloproliferative neoplasm, Polycythemia Vera. The disease was unidentified and with non-specific symptomology until the occurrence of stroke. The particularity of this case is the presence of severe cardiovascular disease (stage III hypertension. Obstructive hypertrophic cardiomyopathy, chronic ischemic silent disease), with cerebral manifestations (stroke) and associated with mixt hepatitis (alcoholic and deficiency), hypercholesterolemia and hyperuricemia. This biological context represent a real challenge both etiologically and therapeutically (pharmacology and non-pharmacology).
Lugol Iodine Staining Test in Early Detection of Head and Neck Cancer
Authors: Stefan Catalin , Lostun Gabriel , Lostun Alexandra
Published: February 2020

Head and neck cancer represents 3% of malignancies, and it is associated with high mortality due to advanced stage diagnosis. In early stages, the symptomatology can either be absent or be very common, misleading the patient who often ignores it. Early diagnosis of head and neck neoplasia is essential for a favorable long-term outcome. Lately new in vivo examination techniques were developed, and older ones have been improved. Today, in vivo staining techniques are an important tool in the diagnostic of head and neck cancer. Lugol iodine staining method provides valuable information concerning the tumor, allowing the surgeon to differentiate premalignant and malignant lesions.
The Use of Deep Eutectic Solvents Ionic Liquids for Selective Dissolution and Recovery of Sn, Pb and Zn from Electric and Electronic Waste (WEEE)
Authors: Popescu Ana Maria , Donath Cristina , Neacsu Elena Ionela , Soare Vasile , Constantin Ionut , Burada Marian , Dumitrescu Daniela Violeta , Yanuskevich Kazimir , Constantin Virgil
Published: February 2020

The recovery of metals from a multi-component alloy obtained by crushing, melting and anodic dissolution of waste electric and electronic equipment (WEEE) has been investigated. The aim of this paper is to selective recover of Sn, Pb and Zn by a novel ecological technology using ionic liquids. Metallic Sn, Pn and Zn were electrochemically recovered from the WEEE dissolved in choline chloride-ethylene glycol-iodine ionic liquid. Cyclic voltammetry was used in order to determine the deposition potentials of the studied metals. XRD and SEM/EDX analysis methods were used to characterize the structure and morphology of the metallic deposits. Evolution of the cathodic deposition and of the chemical composition of the anode during the anodic dissolution process for Sn, Pb and Zn was also studied. This study has demonstrated the possibility of selective recovery of Sn, Pb and Zn from the multi-component alloy (which resulted from consecutive anodic dissolution of WEEE) by anodic dissolution/deposition in ionic liquids.
Influence of Foaming Agent/Water Ratio and Foam/Geopolymer Paste Ratio to the Properties of Fly Ash-based Lightweight Geopolymer for Brick Application
Authors: Ibrahim Wan Mastura Wan , Hussin Kamarudin , Abdullah Mohd Mustafa Al Bakri , Kadir Aeslina Abdul , Deraman Laila Mardiah , Sandu Andrei Victor
Published: February 2020

Nowadays, the demand for lightweight building materials has been growing worldwide. This paper presents an investigation on the use of waste materials of fly ash as a source materials for the production of lightweight geopolymer by using foaming agents. The key properties for the foamed geopolymer namely density, compressive strength, and water absorption were investigated. The chemical composition of materials and morphology analysis were studied to find the microstructure properties of foamed geopolymer. The foamed geopolymer were prepared by combination of 12 M Sodium Hydroxide (NaOH) solution and Sodium Silicate (Na2SiO3) solution. The ratio of Na2SiO3/NaOH and ratio of fly ash/alkaline activator were kept constant at 2.5 and 2.0, by mass respectively. The effect of different ratio of foaming agent/water and foam/geopolymer paste were investigated at 7 days of ageing and cured at 80�C for 24 hours. In general, the results showed that the fly ash-based lightweight geopolymer has good potential as brick application.
Synthetic Phenylpiperidine Derivative Meperidine as a Trigger for Type I Negative Pressure Pulmonary Edema in General Anesthesia
Authors: Baracan Adrian , Gavris Claudia , Poroch Vladimir , Toma Sebastian , Simion Laurentiu , Sandu Ion , Pas Alina Mihaela
Published: February 2020

We present a case of type I negative pressure pulmonary edema in a healthy 17-year-old boy who underwent an emergent appendectomy under general anesthesia. The particularity of our case revolves around the administration of meperidine for perioperative shivering, along with other anesthetic risk factors, which may have served as the trigger of type I negative pressure pulmonary edema.We present the pathophysiological mechanisms, the formulation of clinical and paraclinical diagnosis and the principles of intensive care therapy. This was the first such case experienced in our practice, with a remarkable learning opportunity.
Protective Effect of Hypericum perforatum L. Extract on Hexavalent Chromium Induced Toxicity in Rat Adrenal Gland
Authors: Savici Jelena , Boldura Oana Maria , Balta Cornel , Brezovan Diana , Muselin Florin , Mircu Calin , Borza Claudia , Motoc Marilena , Tulcan Camelia
Published: February 2020

This study was carried out to test the possibility of hexavalent chromium administration through drinking water to induce the structural damage in rat�s adrenal glands and the possibility of Hypericum perforatum extract to faith against chromium aggression. Chromium induced cellular stress was determined by the expression level assessment of the Bcl2 genes family, known to modulate the apoptotic pathway. Obtained results showed that exposure to chromium altered adrenal glands morphology, by induction of apoptosis. When Hypericum perforatum extract was administered expression level of Bcl2 genes and histological lesions in adrenal glands were significantly reduced.
The Effect of Exercising on Oxidative Stress Status and Pain in a Valproic-acid Induced Model of Autism Possible relevance of oxytocin
Authors: Antioch Iulia , Ababei Dana , Lefter Radu , Ciobica Alin , Honceriu Cezar , Bild Veronica
Published: February 2020

In this report we will describe the effect of exercising (6 days, each day 3 separate training phases of 5 minutes each, on an adapted treadmill at 1.0 m/s) on oxidative stress status from the temporal lobe (expressed through 3 main parameters: superoxide dismutase (SOD), glutathione peroxidase (GPX) and malondialdehyde (MDA), as a marker of lipid peroxidation) and pain (as determined through 2 specific behavioural tasks such as hot plate test for the supraspinal acute thermal pain and the intra abdominal Zymosan administration for eliciting a local inflammatory reaction following responses to inflammatory visceral pain) in a rat valproic-acid induced perinatal model of autism, also trying to emphasize a possible implication of oxytocin in this complex pathological picture. We demonstrated here an increased oxidative stress status, as a result of treadmill exercising, in a VPA rat induced model of autism, as demonstrated mainly by a significant decrease in the specific activity of SOD (for the exercised VPA female rats), as well as a significant decrease of GPX specific activity in the male VPA exercised rats, when compared to non-exercised VPA groups, as well as the fact that the aforementioned series of exercises did not resulted in any changes of the pain perception of this rat models of autism, as studied in 2 pain-related behavioural tasks, independent to the gender of the rats with VPA model of autism.
Mean Dietary Fiber Intake of Romanian Adults Results of a Survey Questionnaire
Authors: Tarcea Monica , Fazakas Zita , Szucs Viktoria , Kovacs Zsolt , Nagy Eniko Nemes , Olah Peter , Tilinca Mariana , Guine Raquel
Published: February 2020

The role of dietary fibers is crucial especially for the prevention of degenerative diseases and cancer. The purpose of this study was to find practical mathematical formulas to calculate the mean dietary fiber intake of Romanian adult population. Based on the intakes of vegetables, fruits and whole cereals we calculated the Mean Dietary Fiber Intake/day/person (MDFI). Our research shows that the national average MDFI was 9.8 g fiber/day/person, meaning 38% of Dietary Requirements, and the rest of 62% representing a fiber gap that we have to take into account. Public health programs actions sustained and developed by the Romanian Dietitians should be taken towards increasing the consumption of fiber-rich products associated with an increased frequency of physical activity, because these actions will be helpful in reducing chronic diseases incidence in Romania.
Evaluation of a Skin Carcinoma Xenograft Mice Model Supplemented by Chemical Immunosuppression
Authors: Iftode Andrada , Marti Teodora Daniela , Voicu Mirela , Simu Sebastian , Heghes Alina , Borcan Florin , Motoc Andrei
Published: February 2020

As we know today, cancer is a disease caused by the uncontrolled growth of a single cell. This development is triggered by mutations, changes in DNA that affect genes in such a way that they increase cancer cells. In the present study, an experimental model based on skin carcinoma xenograft mice was developed and tested. There were studied: the mice weight, the tumor volume, and the evolution of different skin parameters such as transepidermal water loss (TWL), erythema and the hydration level of stratum corneum; mice were divided in four groups: a control group, a group of mice injected with physiological saline solution, another one with immunosuppression, and a group of mice with immunosuppression and xenograft of A375 cells. The results indicate that administration of cyclophosphamide significantly reduced the number of neutrophils and lymphocytes and last two mice groups revealed more pronounced modifications of skin parameters compared to first two groups.
The Effect of Chemical Compounds from Cigarettes Smoke on First Trimester Biochemical Markers
Authors: Carabineanu Adrian , Navolan Dan , Birsasteanu Florin , Cretu Octavian , Boia Marioara , Craina Marius , Badiu Diana Liana , Ionescu Crangu Antoniu , Mehedintu Claudia , Vladareanu Simona , Ciohat Ioana , Craciunescu Mihaela , Nemescu Dragos
Published: February 2020

Previous studies showed that certain behavioral and physical parameters influence the concentration of first trimester biochemical markers. The scope of the present article was to study the relationship between smoking and first trimester screening biochemical markers and the capability of the software to counterbalance this influence. Concentrations of pregnancy-associated protein A (PAPP-A) and free b chorionic gonadotropin hormone (free b hCG) were measured in sera of 1554 first trimester pregnant women, 1349 of which were non-smokers and 205 were smokers. First trimester PAPP-A values are lower in smoking compared to non-smoking pregnant women (0.94�0.04 vs. 1.09�0.02, p=0.0004), since smoking seems not to influence free b hCG concentration (1.11�0.07 vs. 1.03�0.02, ns). The software used by us corrected successfully the effect of chemical compounds from cigarette smoke on PAPP-A values.
Detecting the Implant in vitro Microleakage Using Spectrophotometry and Neutral Red Dye
Authors: Hajaj Tareq , Talpos Serban , Ianes Emilia , Candea Adrian , Neagu Adrian , Szuhanek Camelia , Negru Radu , Negrutiu Meda Lavinia , Sinescu Cosmin
Published: February 2020

Implants are one of the most innovative and less invasive prosthetic procedures available. Almost all types of edentations can, nowadays, be treated with an implant-retained prosthetic piece, either fixed or removable. But regardless of the development in the industry over the last decades and the high success rate - estimated at over 95% - there are still some problems concerning single or multi-unit restorations[1-3]. One of the main concerns about implant therapy today is the microleakage phenomenon. The microleakage appears between the implant and the abutment and, due to masticatory forces, it creates a pump effect which can carry the oral bacteria inside the implant, where it�s almost impossible to reach, thus, creating a local inflammation, called peri-implantitis[4-6] . Many authors have tried to identify and quantify the phenomenon by diverse means, but a consensus hasn�t been reached on how or when exactly it appears. In the present study, Neutral Red dye was used to determine the sealing of the implant-abutment interface in dynamic conditions, with the help of the spectrophotometry.
New Azulene Modified Electrodes for Heavy Metal Sensing
Authors: Pop Maria Daniela , Anastasoaie Veronica , Apostoiu Popescu Mariana , Oprisanu Alexandra , Ungureanu Eleonora Mihaela , Cristea Mihaela
Published: February 2020

The electrochemical characterization of 2,6-bis((E)-2-(furan-2-yl)vinyl)-4-(5-isopropyl-3,8-dimethylazulen-1-yl) pyridine has been performed by cyclic voltammetry, differential pulse voltammetry and rotating disk electrode. Modified electrodes were obtained by cycling the potential in the anodic scans or by controlled potential electrolysis (CPE) at different potentials and charges. The modified electrodes have been tested for mercury, cadmium, copper and lead ions detection.
Superposition of Potential Chemical Polluants and Radioisotopes and Their Influence Upon the Environment and Living Organisms
Authors: Jinescu Valeriu V. , Manea Simona Eugenia , Jinescu George , Nicolof Vali Ifigenia
Published: February 2020

Following the activities developed in a nuclear facility result gaseous and liquid radioactive effluents and radioactive solid waste. All these waste contain radioactive isotopes which are potentially pollutants for the environment. In the same time chemicals are, also, pollutants. According to the legislation, discharging of chemicals and radioactive liquid and gaseous effluents into the environment, should meet the requirements of the unrestricted discharge. However, what happens when several pollutants superpose: only chemical pollutants, or only radioactive pollutants, or chemical and radioactive pollutants? Such problems have been solved in this paper on the basis of the principle of critical energy.
Boundary Conditions for the Simulation of Wave Breaking
Authors: Iele Benedetta , Palleschi Federica , Gallerano Francesco
Published: February 2020

In this paper we propose a new numerical model for the simulation of the wave breaking. The three-dimensional equations of motion are expressed in integral contravariant form and are solved on a curvilinear boundary conforming grid that is able to represent the complex geometry of coastal regions. A time-dependent transformation of the vertical coordinate that is a function of the oscillation of the turbulent wave boundary layer is proposed. A new numerical scheme for the simulation of the resulting equations is proposed. New boundary conditions at the free surface and bottom for the equations of motion expressed in contravariant form are proposed. We present an analysis of the importance of the correct positioning, inside the oscillating turbulent boundary layer, of the centre of the calculation grid cell closest to the bottom, in order to correctly simulate the height of the breaking waves.
Comparing the Story Retelling Speaking Test With Other Speaking Tests
Authors: Koizumi Rie , Hirai Akiyo
Published: February 2020

This study examines the validity of score-based interpretation of the Story Retelling Speaking Test (SRST) in comparison with the Versant(Pearson Education, Inc., 2008) and Standard Speaking Test (SST; ALC Press, Inc., 2010). In total, 64 participants took the three tests; their speaking functions, scores, and utterances were analyzed to probe the shared and varied aspects of the tests. The results showed that the SRST elicited more functions than the Versant but fewer than the SST, that it was moderately related to the latter two tests, and that it more successfully discriminated among a group of beginner and intermediate level learners. Additionally, the results suggested that (a) the tasks and speaking functions and (b) the aspects emphasized while ratingmay explain the differencesin the test scores for the three tests.Based on the results, comparative advantages of each test were summarized, which may be useful for selecting appropriate speaking tests according to assessment purposes and situations.

本研究では、Story Retelling Speaking Test (SRST) を、Versant (Pearson Education, Inc., 2008) と Standard Speaking Test (SST; ALC Press, Inc., 2010)と比較し、SRSTの得点に基づく解釈の妥当性を吟味する。64名の受験者が3つのテストを受けた。テストの共通点と相違点を調べるために、その言語機能と得点、発話が分析された。その結果、SRSTはVersantより多いがSSTよりは少ない言語機能を引き出すこと、SRSTは他の2つのテストと中程度の相関があり、初級者・中級者グループで弁別力を持つこと、(a)タスクと言語機能と(b)評価時に強調する要素の相違により、テスト得点の違いが説明されうることが分かった。結果に基づき、評価の目的と状況に沿って適切なスピーキングテストを選ぶ際に有益となる、各テストの相対的な利点を示した。
Willingness to Communicate and Japanese High School English Learners
Authors: Watanabe Michinoku
Published: February 2020

In this longitudinal study I investigated the extent, if any, to which Japanese high school English learners’ willingness to communicate (WTC; McCroskey & Richmond, 1985) changes over the 3 years of high school and then explored the reasons for the changes or stability. A questionnaire was developed drawing on the WTC scale (McCroskey, 1992) and administered to 190 students three times at yearly intervals. The data were analyzed with the Rasch rating scale model and ANOVAs. Two distinct constructs were identified across the three waves of data: willingness to communicate with friends and acquaintances (WTCFA) and willingness to communicate with strangers (WTCS). The results showed that neither WTCFA nor WTCS changed significantly over the high school years, and that WTCS remained low. Follow-up interviews with selected students suggested several possible reasons for the questionnaire results.

本縦断的研究は、日本の高校生英語学習者のwillingness to communicate （WTC; McCroskey & Richmond, 1985）が高校３年間に変化するのかどうかと、その変化または無変化の理由を調査したものである。WTC質問票（McCroskey, 1992）を基に質問票を作成し、高校生190人に、１年間隔で３回、回答してもらった。質問票のデータは、ラッシュモデルと分散分析（ANOVA）を用いて分析した。その結果、何れの回においても、「友人や知人に対するWTC」と「見知らぬ人に対するWTC」の２つの心理概念が見いだされた。そして、「友人や知人に対するWTC」にも「見知らぬ人に対するWTC」にも高校３年間に有意な変化が無かったこと、また、「見知らぬ人に対するWTC」は大変低いレベルに留まっていたことがわかった。質問票での調査に続けて実施した、抽出した生徒へのインタビューでは、それらの結果のいくつかの理由が示唆された。
Perspectives: English as a Lingua Franca and Its Implications for English Language Teaching
Authors: Cheung Chit , Sung Matthew
Published: February 2020

In recent years, the use of English as a Lingua Franca (ELF) worldwide has given rise to the question of whether English as a Native Language (ENL) norms should continue to be used in the English Language Teaching (ELT) classroom. In this paper I explore the controversial issues surrounding the implications of ELF for ELT by considering the practicalities of language teaching and learning. I argue that ELF should not be seen as in competition with ENL or as a replacement of ENL for pedagogical purposes. Instead, ENL and ELF can play different but complementary roles in ELT. Although ENL may remain as the primary model for pedagogy as a point of reference, there is a need for teachers to raise students’ awareness of ELF use in reality, including the notion of language variation in ELF and the role of English in today’s world.

英語がリンガフランカとして世界中で使用されるに至り、ここにひとつの疑問が浮かび上がる。それは、母語としての英語（以下ENL）使用の基準が教室で英語を教える際に使われ続けてもいいのかどうか、という疑問である。本稿では、言語指導そして言語学習における実用性を念頭に、英語というリンガフランカ（以下ELF）の基準を英語教育（以下ELT）の指導に適用することをめぐる問題について考察する。筆者の考えでは、ELFをENLと競争関係にあるもの、あるいは指導を目的としたENLの代用として見なすべきではない。そうではなく、ENLとELFはそれぞれに異なった働きが、しかもELTの場で互いを補い合う働きがある。ENLは指導のための第一義的な参照対象であり続けると思われるが、その一方で、ELFの言語的多様性に対する考え方、そして現代社会における英語の役割を含めたELFの現実そのものを学習者が認識するよう、教師は努める必要がある。