bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of a Two (32/38 Weeks) versus One (36 Weeks) Ultrasound Protocol for the Detection of Decreased Fetal Growth and Adverse Perinatal Outcome
Authors: Nieto-Tous Mar , Novillo-Del Álamo Blanca , Martínez-Varea Alicia , Satorres-Pérez Elena , Morales-Roselló José
Published: July 2024

Third-trimester ultrasound has low sensitivity to small for gestational age (SGA) and adverse perinatal outcomes (APOs). The objective of this study was to compare, in terms of cost-effectiveness, two routine third-trimester surveillance protocols for the detection of SGA and evaluate the added value of a Doppler study for the prediction of APO. This was a retrospective observational study of low-risk pregnancies that were followed by a two growth scans protocol (P2) at 32 and 38 weeks or by a single growth scan at 36 weeks (P1). Ultrasound scans included an estimated fetal weight (EFW) in all cases and a Doppler evaluation in most cases. A total of 1011 pregnancies were collected, 528 with the P2 protocol and 483 with the P1 protocol. While the two models presented no differences for the detection of SGA in terms of sensitivity (47.89% vs. 50% p = 0.85) or specificity (94.97 vs. 95.86% p = 0.63), routine performance of two growth scans (P2) led to a 35% cost increase. The accuracy of EFW for the detection of SGA showed a noteworthy improvement when reducing the interval to labor, and the only parameter with predictive capacity of APO was the cerebroplacental ratio at 38 weeks. In low-risk pregnancies, the higher costs of a two-scan growth surveillance protocol at the third trimester are not justified by an increase in diagnostic effectivity.
Neck Circumference Cut-Off Points for Identifying Adiposity: Association with Chronic Metabolic Diseases in Older People
Authors: Díaz Dominique A. , Lera Lydia , Márquez Carlos , Valenzuela Andrea , Saguez Rodrigo , Weisstaub Gerardo , Albala Cecilia
Published: July 2024

Background: The leading cause of death in older people is cardiovascular diseases. Several studies have found that neck circumference (NC) is a simple anthropometric marker associated with adiposity. The aim of this study is to estimate and validate NC cut-off points as adiposity markers and analyze their association with cardiovascular and chronic metabolic diseases in older people. Methods: A cross-sectional study in 358 non-disabled, community-dwelling older people (71.7 ± 3.9 years) living in Santiago de Chile and participating in the HTSMayor study was conducted. Measurements of body composition and cardiovascular risks were evaluated. Receiver operating characteristic (ROC) curves and multiple logistic regression models were used to evaluate the association of NC with cardiovascular and chronic metabolic diseases. NC cut-off points were obtained to predict obesity, abdominal obesity, and adiposity. Results: The best performance values of neck circumference relative to obesity and adiposity were obtained with respect to abdominal obesity (40.6 cm in men and 34.2 cm in women). Higher NC values were associated with a higher area under the curve (AUC) for men and women (men: AUC = 0.84; women: AUC = 0.86). NC was significantly associated with a higher risk for diabetes mellitus (OR = 1.95), hypertension (OR = 2.42), acute myocardial infarction (OR = 4.36), and comorbidities (OR = 2.01), and a lower risk for sarcopenia (OR = 0.35). Conclusions: This study shows that NC is a useful tool for detecting abdominal obesity, obesity, and adiposity in older people and that a higher NC increases the risk of chronic diseases.
Empowerment, Pain Control, and Quality of Life Improvement in Early Triple-Negative Breast Cancer Patients through Pain Neuroscience Education: A Prospective Cohort Pilot Study Protocol (EMPOWER Trial)
Authors: Tiberio Paola , Balordi Marco , Castaldo Matteo , Viganò Alessandro , Jacobs Flavia , Benvenuti Chiara , Torrisi Rosalba , Zambelli Alberto , Santoro Armando , De Sanctis Rita
Published: July 2024

The treatment of early triple-negative breast cancer (eTNBC) has improved patients’ prognosis but often leads to adverse events and sequelae affecting quality of life (QoL). Pain Neuroscience Education (PNE) is a promising non-pharmacological intervention in this field. Preliminary data have shown the beneficial effect of PNE in BC survivors. However, there are still gaps in knowledge regarding its optimal use in eTNBC. To address this issue, a prospective pilot study will enroll 30 consecutive patients diagnosed with eTNBC at IRCCS Humanitas Research Hospital. The PNE program will consist of 10 weekly sessions to be started within 4 weeks of the onset or worsening of a pain syndrome (PS). QoL, pain, and disability will be assessed before, during, at the end of, and 6 months after PNE using validated questionnaires. Peripheral venous blood samples will be taken before and at the end of PNE to evaluate inflammatory serum biomarker levels. The primary objective is to evaluate whether PNE leads to clinical improvement in QoL and pain. If successful, it will be validated in a larger multi-centric cohort, potentially leading to its widespread implementation as a standard pain management tool for eTNBC patients.
Efficacy and Safety of Filgotinib in Rheumatoid Arthritis Patients Aged over and under 65 Years (ENANTIA-65)
Authors: Benucci Maurizio , Bardelli Marco , Cazzato Massimiliano , Bartoli Francesca , Damiani Arianna , Li Gobbi Francesca , Bandinelli Francesca , Panaccione Anna , Di Cato Luca , Niccoli Laura , Frediani Bruno , Mosca Marta , Guiducci Serena , Cantini Fabrizio
Published: July 2024

Background: According to recent data, the age of patients could represent an important risk factor for MACE (major cardiovascular events), cancer, and VTE (venous thromboembolism) during treatment with JAK inhibitors in rheumatoid arthritis. We decided to analyze the population involved in the ReLiFiRa study by identifying two groups of patients: 65 years or more and less than 65 years of age, evaluating the efficacy and tolerability of 200 mg of Filgotinib daily. Methods: Of the 120 ReLiFiRa patients, 54 were younger than 65 years old and 66 patients were 65 years old or older. The data of efficacy and tolerability of treatment with FIL 200 mg daily for 6 months were evaluated. Results: After six months of treatment, FIL was effective in both age groups. In both groups, the median values of steroid DAS28, CDAI, ERS, PCR, tender joints, swollen joints, VAS, HAQ, PGA patients, and PGA physicians were reduced with a statistically significant difference comparing these values with the baseline values. The difference in age did not impact the effectiveness of the drug. The lipid profile data also did not demonstrate significant differences between the two age groups; however, the comparison between younger vs. older patients’ populations regarding the total cholesterol/HDL ratio and LDL/HDL ratio shows a statistically significant difference: total cholesterol/HDL 3.4 (2.12–3.66) vs. 3.64 (3.36–4.13) p = 0.0004, LDL/HDL 1.9 (0.98–2.25) vs. 2.41 (2.04–2.73) p = 0.0002. There are no differences regarding the atherogenic index (LDL-C/HDL-C) and coronary risk index (TC/HDL-C) compared to baseline. Conclusions: After six months of treatment with FIL, the older population group showed a higher level of LDL and a lower level of HDL compared to younger patients. The atherogenic index and coronary risk index are higher in patients aged ≥ 65 years, but interestingly, there were no differences when comparing the 6-month data to baseline values. This condition highlights the impact of typical risk factors that act independently of treatment with Filgotinib.
An Integrated Event-Driven Real-Time Tactical–Operational Optimization Framework for Smart Port Operations Planning
Authors: Abril Danilo , Paternina-Arboleda Carlos D. , Velasquez-Bermudez Jesus
Published: July 2024

The ongoing issues in global supply chain disruptions have raised many concerns of port productivity, among which port congestion is a key issue. This article implements an integrated tactical–operational optimization framework which raises the capabilities of port information systems to deliver smarter decision-making processes in ports through a decision support system. To this end, we developed a library of multiple smart models for the optimization of port operations, independently engaged in parallel but mathematically coordinated to achieve autonomous real-time distributed optimization, using a novel event-driven structure to enable future implementations using digital twins. The framework was tested to benchmark different commercial solvers on several real instances for the port under study. The results show a strong improvement in port operational planning.
Artificial Neural Network Prediction of Compliance Coefficients for Composite Shear Keys of Built-Up Timber Beams
Authors: Ladnykh Irene A. , Ibadov Nabi , Anysz Hubert
Published: July 2024

This article explores the possibility of predicting the compliance coefficients for composite shear keys of built-up timber beams using artificial neural networks. The compliance coefficients determine the stresses and deflections of built-up timber beams. The article analyzes current theoretical methods for designing wooden built-up timber beams with shear keys and possible ways of applying them in modern construction. One of the design methods, based on the use of the compliance coefficients, is also discussed in detail. The novelty of this research is that the authors of the article collected, analysed, and combined data on the experimental values of the compliance coefficient for composite shear keys of built-up timber beams obtained by different researchers and published in other studies. For the first time, the authors of this article generated a table of input and output data for predicting compliance coefficients based on the analysis of the literature and collected data by the authors. As a result of this research, the article’s authors proposed an artificial neural network (ANN) architecture and determined the mean absolute percentage error for the compliance coefficients kw and ki, which are equal to 0.054% and 0.052%, respectively. The proposed architecture can be used for practical application in designing built-up timber beams using various composite shear keys.
Research on the Analysis of Residual Stress in Heat Treatment of Bellows Using ABAQUS
Authors: Wang Anheng , Ling Chuanwen , Zhao Xiang , Wang Hui , Wang Tao , Tao Guangming , Fu Yanchao , Cheng Tao
Published: July 2024

Taking austenitic stainless-steel bellows as the research object, a finite element model for the heat treatment of austenitic stainless-steel bellows was constructed based on ABAQUS CAE 2022. The physical properties of the bellows after the heat treatment were analyzed using experimental and simulated curve processing analysis methods. The changes in residual stress and deformation in relation to the bellows under different cooling times were explored, as well as the distribution of residual stress and deformation at a certain cooling time. The results show that as the cooling time of the heat treatment increases, the residual stress of the bellow decreases significantly, the reduction rate accelerates, and the degree of deformation gradually decreases. When the cooling time of the heat treatment is 900 s, the residual stress of the wave peak in the middle position of the bellow is relatively small, and the residual stress value of the wave valley along the axis direction does not change significantly. The deformation degree of the wave peak and valley axis direction is relatively uniform.
Two Schemes of Impulsive Runge–Kutta Methods for Linear Differential Equations with Delayed Impulses
Authors: Zhang Gui-Lai , Liu Chao
Published: July 2024

In this paper, two different schemes of impulsive Runge–Kutta methods are constructed for a class of linear differential equations with delayed impulses. One scheme is convergent of order p if the corresponding Runge–Kutta method is p order. Another one in the general case is only convergent of order 1, but it is more concise and may suit for more complex differential equations with delayed impulses. Moreover, asymptotical stability conditions for the exact solution and numerical solutions are obtained, respectively. Finally, some numerical examples are provided to confirm the theoretical results.
Optimization of Interaction with Counterparties: Selection Game Algorithm under Uncertainty
Authors: Zaytsev Andrey , Mihel Ekaterina , Dmitriev Nikolay , Alferyev Dmitry , Laszlo Ungvari
Published: July 2024

The purpose of this study is to develop a comprehensive algorithm for optimizing the interaction of economic entities with counterparties, taking into account the uncertainty of market conditions and the variety of behavioral strategies of participants. The developed algorithm aims to increase the stability and efficiency of the interactions between the economic entity under study and its counterparties, minimizing risks and optimizing cooperative and competitive strategies within the framework of existing market relations. The methodology uses game theory to devise interaction strategies using mutual influence indices, non-cooperative game principles, and payment matrices. The model analyzes various interaction scenarios with counterparties by using payment matrices and considering both competitive and cooperative conditions. The research methodology is supplemented by the calculation of integral estimates based on a set of financial and economic indicators, enabling the assessment of the impact of various interaction strategies on the overall efficiency of an economic entity. After testing the developed models, a set of data was obtained, which can be used to optimize strategic planning and manage the interaction of economic entities with counterparties. The developed algorithm is an effective tool for improving the operational analysis of enterprises, primarily in industrial sectors.
Obtaining Conservative Estimates of Integrated Profitability for a Single-Period Product in an Own-Branding-and-Manufacturing Enterprise with Multiple Owned Channels
Authors: Su Rung-Hung , Hou Chia-Ding , Lee Jou-Yu
Published: July 2024

The achievable capacity index (ACI) is a simple and efficient approach for estimating the profitability of newsboy-type products, wherein profitability is defined as the probability of achieving the target profit by optimizing the order quantity. At present, the ACI is applicable to single retail stores (i.e., single demand) but not to multiple sales channels (i.e., multiple demand). This paper presents an integrated achievable capacity index (IACI) by which to measure the aggregate profitability of multiple mutually independent channels under normally distributed demand. An unbiased IACI estimator is also developed, to which is applied the Taylor expansion to approximate its sampling distribution, wherein the sizes, means, and variances of demand differ in each channel. Furthermore, overestimates due to sampling error are avoided by deriving the lower confidence bound for the IACI. This paper also provides generic tables to aid managers seeking conservative estimates of profitability. The applicability of the proposed scheme is demonstrated numerically using a real-world example involving an own-branding-and-manufacturing (OBM) enterprise with multiple owned channels.
Narcotic Nitrogen Effects Persist after a Simulated Deep Dive
Authors: Dreyer Sven , Schneppendahl Johannes , Hoffmanns Martin , Muth Thomas , Schipke Jochen D.
Published: July 2024

Background and Objectives: Scuba divers often experience persistent inert gas narcosis (IGN) even after surfacing. This study aimed to test the hypothesis that breathing oxygen (O2) before surfacing can reduce postdive IGN. Materials and Methods: A group of 58 experienced divers underwent a 5 min dive at a depth of 50 m in a multi-place hyperbaric chamber. They were decompressed using air (air group). Another group of 28 divers (O2 group) breathed 100% O2 during the end of decompression. Prior to and after the dive, all participants performed the Sharpened Romberg test (SRT) and a modified tweezers test. Results: In the air group, the number of positive SRT results increased postdive (47% vs. 67%), indicating a greater impairment in the vestibular system (Cohen’s d = 0.41). In the O2 group, the percentage of positive SRT results remained constant at 68% both before and after the dive. In terms of the modified tweezers test, the air group showed no significant change in the number of picked beads (40 ± 9 vs. 39 ± 7), while the O2 group demonstrated an increase (36 ± 7 vs. 44 ± 10) (Cohen’s d = 0.34). Conclusion: The results reveal that the SRT revealed a negative effect of nitrogen (N2) on the vestibular system in the air group. The increased number of beads picked in the O2 group can be attributed to the learning effect, which was hindered in the air group. Consistent with our hypothesis, breathing O2 during decompression appears to reduce postdive IGN.
Genomic Insights into and In Vitro Evaluation of Antimicrobial Combination Therapies for Carbapenem-Resistant Acinetobacter baumannii
Authors: Ijaz Saadia , Ansari Farheen , Nawaz Muhammad , Ejaz Hasan , Anjum Aftab Ahmad , Saeed Aqib , Ali Tehreem , Rehman Obaid Ur , Fatima Eeshal , Ijaz Tayyaba
Published: July 2024

Background and Objectives: Acinetobacter baumannii (A. baumannii), particularly carbapenem-resistant A. baumannii (CRAB), represents a grave concern in healthcare settings and is associated with high mortality. This study aimed to conduct molecular, mutational, and phylogenetic analyses of specific genes in CRAB and evaluate the synergistic effects of selected antimicrobial combinations. Materials and Methods: Phenotypic characterization was performed on six CRAB strains by using the Modified Hodge Test (MHT) and IMP-EDTA Double-Disc Synergy Test (IMP-EDTA DDST). Carbapenemase- and metallo-beta-lactamase-encoding genes were amplified by using Polymerase Chain Reaction. Phylogenetic analysis using the MEGA 11 tool was used to determine the evolutionary relatedness of these genes. Mutational analysis was performed by using I-Mutant, MUPro, and PHD-SNP bioinformatics tools to predict mutations in the carbapenemase-encoding genes. Microdilution checkerboard titration assessed the synergistic effects of antimicrobial combinations (azithromycin–meropenem, rifampicin–meropenem, meropenem–colistin, and azithromycin–colistin) on these CRAB isolates. Results: The phenotypic characterization of six CRAB isolates revealed positive results for MHT and IMP-EDTA DDST. The molecular characterization revealed that carbapenemase- and MBL-encoding genes were present in all isolates with varying frequencies, including blaOXA-51 (100%) and blaIMP (0%). The sequence analysis revealed high evolutionary relatedness to sequences in the NCBI database. The mutational analysis identified 16 mutations, of which 1 mutation (P116L) in the blaOXA-58 gene predicted a change in the protein product, potentially contributing to carbapenem resistance. The checkerboard titration method did not reveal any synergism among the tested antimicrobial combinations against CRAB. Conclusion: This study’s findings underscore the significant challenges posed by CRAB isolates harboring multiple resistant genes in treatment. This highlights the urgent need for novel antimicrobial agents, a crucial step towards reducing mortality rates not only in Pakistan but also globally.
Experimental Studies on the Anisotropic Fatigue Behaviour of IN718 Fabricated via Wire Arc Additive Manufacturing
Authors: Wu Guiyi , Yang Maohong , Yu Zhaohui , Zhang Shuyan , Liu Hongbo , Xiong Jun
Published: July 2024

Wire and arc additive manufacturing (WAAM) offers promise in creating large complex structures due to its flexibility and high material deposition rates. The nickel-based alloy IN718 is favoured for WAAM due to its weldability and compatibility. However, WAAM can introduce issues like anisotropic grain structure, porosity, and residual stresses which can lead to directional variations in tensile, fatigue, and fracture behaviour. This paper studied the WAAM process of IN718, utilising cold metal transfer (CMT). The optimised CMT-WAAM parameters for IN718 were identified to as a wire feed speed of 8–10 m/min and a torch travel speed of 0.5–0.7 m/min, resulting in stable deposition and minimal defects. Nevertheless, columnar grain structures were observed in the build direction (BD), with coarse grains in the wall-length direction (WD). This anisotropic microstructure coupled with stress concentrators, contributes to the directional dependence observed in tensile properties, fatigue endurance, and crack growth. The investigation revealed superior ductility in the BD compared to the WD. Interestingly, the fatigue endurance testing showed a longer life in the WD compared with the BD, attributed to stronger stress concentrators in the BD specimens. However, when examining a cracked specimen, the fatigue crack propagated faster in the WD rather than the BD.
Microstructure Evolution and Mechanical Properties of Extruded AlSiCuFeMnYb Alloy
Authors: Ji Xiaohu , Xiong Junjie , Zhou Lihua
Published: July 2024

This study investigates the impact of varying extrusion ratios on the microstructure and mechanical properties of AlSiCuFeMnYb alloy. Following hot extrusion, significant enhancements are observed in the microstructure of the cast rare earth aluminium alloy. Within the cross-sectional microstructure, the α-Al phase is reduced in size, and its dendritic morphology is eliminated. The morphology of the eutectic Si phase transitions from long strips to short rods, fine fibres, or granular forms. Similarly, the Fe-rich phase changes from a coarse skeletal and flat noodle shape to small strips and short skeletal forms resembling Chinese characters. The CuAl2 phase evolves from large blocks to smaller blocks and granular forms, while the Yb (Ytterbium)-rich rare earth phase shifts from large blocks to smaller, more uniformly distributed blocks. In the longitudinal section, the structure aligns into strips along the extrusion direction, with the spacing between these strips decreasing as the extrusion ratio increases. At an extrusion ratio of 22.56, the alloy demonstrates superior mechanical properties with a tensile strength of 325.50 MPa, a yield strength of 254.44 MPa, a hardness of 143.90 HV, and an elongation of 15.47%. These represent improvements of 27.8%, 36.5%, 38.9%, and 236.4%, respectively, compared with the as-cast rare earth alloy. In addition, the fracture surface of the extruded rare earth alloy exhibits obvious ductile fracture characteristics. Additionally, the alloy undergoes dynamic recrystallisation and dislocation entanglement during hot extrusion. The emergence of a twinned Si phase and a dynamically precipitated nanoscale CuAl2 phase are critical for enhancing deformation strengthening, modification strengthening, and dynamic precipitation strengthening of the extruded alloys.
Influence of Copper Addition on the Mechanical Properties and Corrosion Resistance of Self-Hardening Secondary Aluminium Alloy AlZn10Si8Mg
Authors: Mikolajčík Martin , Kuchariková Lenka , Tillová Eva , Sanchez Jon Mikel , Šurdová Zuzana , Chalupová Mária
Published: July 2024

Aluminium alloys have a wide range of applications, mainly due to their advantageous strength-to-weight ratio, denoted as specific strength and corrosion resistance. In recent decades, there has been a notable surge in the usage of recycled alloys, attributed to their reduced production costs and emissions. One of the conditions for secondary production is the optimal sorting of used scrap. Once the aluminium scrap has been melted, it is tough to reduce the content of the various additives. Copper is the primary alloying element in some aluminium alloys, which leads to an increased amount of copper in the aluminium scrap. Therefore, it is important to investigate its effect on the properties of aluminium alloys in which it is not commonly present. For this reason, this paper is concerned with the influence of copper on the microstructure and properties of the secondary aluminium alloy AlZn10Si8Mg. Specifically, it compares two melts of self-hardening AlZn10Si8Mg alloys differing in copper content (0.019% and 1.72%). A complex quantitative and metallographic analysis by optical and electron microscopy has been performed. Mechanical properties were investigated by tensile test, Brinell hardness, and Vickers microhardness measurements. The corrosion resistance of the individual melts was verified by the Audi test.
Mechanisms of Low-Temperature Dislocation Motion in High-Entropy Al0.5CoCrCuFeNi Alloy
Authors: Semerenko Yuri O. , Natsik Vasilij D. , Tabachnikova Elena D. , Huang Yi , Langdon Terence G.
Published: July 2024

An analysis of the processes of plastic deformation and acoustic relaxation in a high-entropy alloy, Al0.5CoCrCuFeNi, was carried out. The following were established: dominant dislocation defects; types of barriers that prevent the movement of dislocations; mechanisms of thermally activated movement of various elements of dislocation lines through barriers at room and low temperatures. Based on modern dislocation theory, quantitative estimates were obtained for the most important characteristics of dislocations and their interaction with barriers.
Evaluation of Lattice Structures for Medical Implants: A Study on the Mechanical Properties of Various Unit Cell Types
Authors: Nogueira Pedro , Lopes Pedro , Oliveira Luís , Alves Jorge L. , Magrinho João P. G. , Deus Augusto Moita de , Vaz M. Fátima , Silva M. Beatriz
Published: July 2024

Lattice structures are a prime candidate for applications in the medical implant industry due to their versatile mechanical behaviour, which can be tailored to meet specific patient needs and reduce stress shielding, while enabling the natural flow of body fluids. In this work, the mechanical properties of metallic lattices made of five different unit cell types, Cubic (C), Truncated Octahedron (TO), Truncated Cubic (TC), Rhombicuboctahedron (RCO), and Rhombitruncated Cuboctahedron (RTCO), were evaluated under uniaxial compression at three different relative densities, 5%, 15%, and 45%. The evaluation was experimental, and it was compared with previous and new finite element simulations. Specimens for the experimental tests were fabricated in stainless steel 316L by laser powder bed fusion, and stress–strain curves were obtained for the different lattices. The combination of the test results with a critical interpretation of the deformation mechanisms allowed us to confirm that two unit cell types, TO and RTCO, are stable for the whole range of relative densities evaluated. The other three unit cells exhibit more unpredictable behaviour, either due to manufacturing defects or limitations, or because their unstable compression behaviour leads to bucking. For these reasons, TO and RTCO unit cell types are mechanically more adequate for applications in the medical implant industry.
Gut Microbial Adaptation to Varied Altitudes and Temperatures in Tibetan Plateau Yaks
Authors: Zhu Yanbin , Wang Jia , Cidan Yangji , Wang Hongzhuang , Li Kun , Basang Wangdui
Published: July 2024

The yak (Bos grunniens) exhibits exceptional regional adaptability, enabling it to thrive in the distinctive ecological niches of the Qinghai–Tibet Plateau. Its survival relies on the intricate balance of its intestinal microbiome, essential for adapting to harsh environmental conditions. Despite the documented significance of bacteria and fungi in maintaining intestinal homeostasis and supporting immune functions, there is still a substantial gap in understanding how the composition and functionality of yak gut microbiota vary along altitude–temperature gradients. This study aims to fill this gap by employing 16S rRNA and ITS amplicon sequencing techniques to analyze and compare the intestinal microbiome of yaks residing at different elevations and exposed to varying temperatures. The findings demonstrate subtle variations in the diversity of intestinal bacteria and fungi, accompanied by significant changes in taxonomic composition across various altitudes and temperature gradients. Notably, Firmicutes, Actinobacteriota, and Bacteroidota emerged as the dominant phyla across all groups, with Actinobacteriota exhibiting the highest proportion (35.77%) in the LZF group. Functional prediction analysis revealed significant associations between the LZF group and metabolic pathways related to amino acid metabolism and biosynthesis. This suggests a potential role for actinomycetes in enhancing nutrient absorption and metabolism in yaks. Furthermore, our findings suggest that the microbiota of yaks may enhance energy metabolism and catabolism by modulating the Firmicutes-to-Bacteroidota ratio, potentially mitigating the effects of temperature variations. Variations in gut bacterial and fungal communities among three distinct groups were analyzed using metagenomic techniques. Our findings indicate that microbial genera exhibiting significant increases in yaks at lower altitudes are largely beneficial. To sum up, our research investigated the changes in gut bacterial and fungal populations of yaks residing across diverse altitude and temperature ranges. Moreover, these results enhance comprehension of gut microbial makeup and variability, offering perspectives on the environmental resilience of dry lot feeding yaks from a microbial angle.
Biofilm Formation, Motility, and Virulence of Listeria monocytogenes Are Reduced by Deletion of the Gene lmo0159, a Novel Listerial LPXTG Surface Protein
Authors: Shi Weidi , Zhang Qiwen , Li Honghuan , Du Dongdong , Ma Xun , Wang Jing , Jiang Jianjun , Liu Caixia , Kou Lijun , Ren Jingjing
Published: July 2024

Listeria monocytogenes (L. monocytogenes) is a foodborne pathogen that causes listeriosis in humans and other animals. Surface proteins with the LPXTG motif have important roles in the virulence of L. monocytogenes. Lmo0159 is one such protein, but little is known about its role in L. monocytogenes virulence, motility, and biofilm formation. Here, we constructed and characterized a deletion mutant of lmo0159 (∆lmo0159). We analyzed not only the capacity of biofilm formation, motility, attachment, and intracellular growth in different cell types but also LD50; bacterial load in mice’s liver, spleen, and brain; expression of virulence genes; and survival time of mice after challenge. The results showed that the cross-linking density of the biofilm of ∆lmo0159 strain was lower than that of WT by microscopic examination. The expression of biofilm-formation and virulence genes also decreased in the biofilm state. Subsequently, the growth and motility of ∆lmo0159 in the culture medium were enhanced. Conversely, the growth and motility of L. monocytogenes were attenuated by ∆lmo0159 at both the cellular and mouse levels. At the cellular level, ∆lmo0159 reduced plaque size; accelerated scratch healing; and attenuated the efficiency of adhesion, invasion, and intracellular proliferation in swine intestinal epithelial cells (SIEC), RAW264.7, mouse-brain microvascular endothelial cells (mBMEC), and human-brain microvascular endothelial cells (hCMEC/D3). The expression of virulence genes was also inhibited. At the mouse level, the LD50 of the ∆lmo0159 strain was 100.97 times higher than that of the WT strain. The bacterial load of the ∆lmo0159 strain in the liver and spleen was lower than that of the WT strain. In a mouse model of intraperitoneal infection, the deletion of the lmo0159 gene significantly prolonged the survival time of the mice, suggesting that the lmo0159 deletion mutant also exhibited reduced virulence. Thus, our study identified lmo0159 as a novel virulence factor among L. monocytogenes LPXTG proteins.
Endophytic Fungi for Crops Adaptation to Abiotic Stresses
Authors: Morales-Vargas Adan Topiltzin , López-Ramírez Varinia , Álvarez-Mejía Cesar , Vázquez-Martínez Juan
Published: July 2024

Endophytic fungi (EFs) have emerged as promising modulators of plant growth and stress tolerance in agricultural ecosystems. This review synthesizes the current knowledge on the role of EFs in enhancing the adaptation of crops to abiotic stress. Abiotic stresses, such as drought, salinity, and extreme temperatures, pose significant challenges to crop productivity worldwide. EFs have shown remarkable potential in alleviating the adverse effects of these stresses. Through various mechanisms, including the synthesis of osmolytes, the production of stress-related enzymes, and the induction of plant defense mechanisms, EFs enhance plant resilience to abiotic stressors. Moreover, EFs promote nutrient uptake and modulate the hormonal balance in plants, further enhancing the stress tolerance of the plants. Recent advancements in molecular techniques have facilitated the identification and characterization of stress-tolerant EF strains, paving the way for their utilization in agricultural practices. Furthermore, the symbiotic relationship between EFs and plants offers ecological benefits, such as improved soil health and a reduced dependence on chemical inputs. However, challenges remain in understanding the complex interactions between EFs and host plants, as well as in scaling up their application in diverse agricultural systems. Future research should focus on elucidating the mechanisms underlying endophytic-fungal-mediated stress tolerance and developing sustainable strategies for harnessing their potential in crop production.
Barite Replacement as a Key Factor in the Genesis of Sediment-Hosted Zn-Pb±Ba and Barite-Sulfide Deposits: Ore Fluids and Isotope (S and Sr) Signatures from Sediment-Hosted Zn-Pb±Ba Deposits of Iran
Authors: Rajabi Abdorrahman , Mahmoodi Pouria , Alfonso Pura , Canet Carles , Andrew Colin , Azhdari Saeideh , Rezaei Somaye , Alaminia Zahra , Tamarzadeh Somaye , Yarmohammadi Ali , Khan Mohammadi Ghazaleh , Saeidi Rasoul
Published: July 2024

Iran hosts more than 350 Precambrian to Cenozoic sediment-hosted Zn-Pb±Ba and barite-sulfide deposits, including shale-hosted massive sulfide (SHMS, also called SEDEX) and Irish-type and Mississippi Valley-type (MVT) mineralization, and barite is a common mineral in these deposits. In the SHMS deposits, barite is typically found as fine-grained disseminations in thin laminae. In these deposits, the sulfide laminae often occur as diagenetic replacements and as bands containing authigenic and diagenetic barite and pyrite framboids. In the Irish-type Zn-Pb-Ba and stratabound barite-sulfide deposits, barite exhibits various textures, including fine-grained disseminated barite, banded zebra textures, veins, and massive barite lenses. In some of the giant Irish-type deposits, as well as in the stratabound barite-sulfide mineralization, the main stratabound sulfide ore is developed within a barite envelope and is characterized by the replacement of barite and pyrite by chalcopyrite, galena, and sphalerite. In the MVT deposits, the formation of barite is often related to dolomitization, and sulfide mineralization involves the replacement of the dolomitized carbonate rocks, as well as associated barite. Fluid inclusion studies on the Irish-type deposits indicate that the temperatures and salinities of the sulfide-forming fluids are higher compared to those of the barite-forming fluids. Fluid inclusion analyses of coarse-grained barites from Irish and MVT deposits reveal their hydrothermal origin. The δ3⁴S values of sulfide minerals (pyrite, sphalerite, and galena) in Irish-type deposits exhibit a broad range of low values (mostly −28 to +5‰), primarily revealing a process of bacterial sulfate reduction (BSR). However, the textures (replacement, colloform, and banded) and more positive sulfur isotope values (+1 to +36‰) in the SHMS Zn-Pb deposits suggest that bacterial sulfate reduction (BSR) plays a less significant role. We suggest that thermochemical sulfate reduction (TSR) connected to the direct replacement of barite plays a more relevant role in providing sulfur for the sulfide mineralization in the SHMS, barite-sulfide, and MVT deposits. Based on the textual evidence, sulfur isotopic data, and fluid inclusion studies, barite has been identified as a key controller for the subsequent Zn-Pb mineralization by providing a suitable host and significant sulfur contribution in the sediment-hosted Zn-Pb and stratabound barite-sulfide deposits. This implies that diagenetic barite might be a precursor to all types of sediment-hosted Zn-Pb mineralization.
Mineralogy of Gold, Tellurides and Sulfides from Lianzigou Gold Deposits in the Xiaoqinling Region, Central China: Implications for Ore-Forming Conditions and Processes
Authors: Weng Guoming , Liu Jiajun , Carranza Emmanuel John M. , Zhang Fangfang , Zhai Degao , Wang Yinhong , Gao Shen , Si Mingyang , Su Zaixin , Zhang Yingying
Published: July 2024

The Lianzigou deposit, which has an Au–Te paragenetic association, is hosted in plagioclase gneiss of the Qincanggou Formation in the Taihua Group in the Xiaoqinling region, central China. This quartz vein-type Au deposit comprises native Au and a variety of tellurides. The latter include calaverite (AuTe2), krennerite (Au3AgTe8), petzite (Au3AgTe2), hessite (Ag2Te), melonite (NiTe2), and altaite (PbTe). Four stages have been recognized in this deposit: stage I consists of K-feldspar and quartz; stage II is of milky quartz veins accompanied by coarse-grained disseminated and lumps of pyrite with weak Au mineralization; stage III is composed mainly of Au, tellurides, and sulfides; and stage IV is characterized by abundant carbonate and quartz. Based on mineral assemblage and thermodynamic data, we estimated the physicochemical conditions of the main metallogenic stages. Based on thermodynamic modelling, the physicochemical conditions of Au–Ag–Te mineral associations were estimated. The Au–Ag–Te minerals from stage III formed mainly under conditions of logƒO2 = −43.15 to −33.31, logƒH2S = ~−9.29, pH < 7, logfTe2 = −10.6 to −9.8 and logαAu+/αAg+ = −7.2 to −6.5. In contrast, the physicochemical conditions of stage II were higher, specifically pH (8.3–8.5) and logƒO2 (−34.90−31.96). In the ore-forming fluids of the Lianzigou deposit, the dominant Au species was Au(HS)2− while the dominant Te species were HTe−(aq) and Te22−(aq). Moreover, the Au–Ag–Te metal associations in the Lianzigou Au deposit were derived from mantle materials related to lithospheric thinning of the eastern North China craton in the Early Cretaceous under an extensional tectonic system.
Mineralogy and Geochemistry of Titaniferous Iron Ores in El-Baroud Layered Gabbros: Fe-Ti Ore Genesis and Tectono-Metallogenetic Setting
Authors: Khedr Mohamed Zaki , Moftah Ahmed , El-Shibiny N. H. , Tamura Akihiro , Tan Wei , Ichiyama Yuji , Takazawa Eiichi , Kahal Ali Y. , Abdelrahman Kamal
Published: July 2024

The Neoproterozoic pyroxene gabbros and gabbronorites in the El-Baroud mafic intrusion in the Northern Eastern Desert (NED) of Egypt host Fe-Ti oxide ore deposits. This study discusses the major and trace elements of both titaniferous iron ores and their host rocks, along with the mineral chemistry (major and in situ trace elements) of interstitial clinopyroxene (Cpx), to gain a deeper understanding of the Fe-Ti oxide genesis. These ores occur as disseminated (55–60 vol.% of Fe-Ti oxides) and massive types (85–95 vol.%) in the form of the dyke, layer, and lens. They are composed of titanomagnetite (80–87 vol.%) with subordinate ilmenite (10–15 vol.%) and magnetite (3–5 vol.%), in accordance with their high Fe2O3 (75.66 wt.% on average) and TiO2 contents (16.30–17.60 wt.%). The Cpx in the investigated ores is diopside composition (Mg#; 0.72–0.83) and exhibits a nearly convex upward REE pattern, similar to Cpxs in the ferropicrite that originated from the primitive mantle. Melts in equilibrium with this Cpx resemble Greenstone ferropicrite melts; the parent melt of El-Baroud gabbros is possibly a ferropicritic melt that was derived from the lithospheric mantle during plume interaction. The El-Baroud gabbroic rocks were generated during the arc rifting and crystallized under a high oxygen fugacity at a temperature of 800–1000 °C and a pressure of 3 kbar with a depth of 12 km. The Fe-Ti oxide ores have been formed from ferropicritic parent melts by two processes, including in situ crystallization that leads to the formation of disseminated Fe-Ti oxides in the iron-rich gabbros at the bottom and liquid immiscibility that is responsible for the formation of thick Fe-Ti ore lenses and layers at the top of the gabbroic intrusion. Initially, titanomagnetite crystallized from the primary Ti-rich oxide melt. As cooling progressed, some of the excess titanium in this melt was exsolved in the form of the exsolution ilmenite lamellae within the titanomagnetite. The Fe-Ti oxide layers in the NED follow the trend of NW-SE (Najd trend), where their distribution is possibly controlled by the composition of parent melts (rich in Ti and Fe), high oxygen fugacity, and the structure related to the Najd fault system. The distribution of Fe-Ti oxide ores increases from the NED to the Southern Eastern Desert (SED), suggesting the dominant mantle plumes and/or shear zones in the SED relative to the NED.
Phlogopite 40Ar/39Ar Geochronology for Guodian Skarn Fe Deposit in Qihe–Yucheng District, Luxi Block, North China Craton: A Link between Craton Destruction and Fe Mineralization
Authors: Feng Qiwei , Gao Mingbo , Fu Chao , Li Siyuan , Li Yadong , Gao Jilei , Ma Ming , Wang Zhaozhong , Zhu Yidan , Wu Binglu , Duan Zhuang , Dang Zhicai
Published: July 2024

The Guodian Fe deposit is representative of the newly discovered Qihe–Yucheng high-grade Fe skarn ore cluster, Luxi Block, eastern North China Craton (NCC). The age of the Pandian Fe deposit remains elusive, which hinders the understanding of its metallogenic tectonic background. Phlogopites are recognized in syn-ore stages, and they are closely associated with magnetite in the Guodian skarn Fe deposit. Here, we carried out 40Ar/39Ar dating of phlogopite, which can place a tight constraint on the timing of Guodian iron mineralization and shed light on the geodynamic framework under which the Guodian Fe deposit formed. Ore-related phlogopite 40Ar/39Ar dating yielded 40Ar/39Ar plateau ages of 131.6 ± 1.7 Ma at 890–1400 °C, with the corresponding isochron age being 131.1 ± 2.6 Ma. These two ages are consistent within the error, indicating that they can represent the formation age of the Guodian iron deposit. The mineralization age overlaps the zircon U-Pb age of 124.4 Ma for ore-related Pandian pluton. This age consistency confirms that the iron skarn mineralization is temporally and likely genetically related to Pandian diorite. The present results, coupled with existing isotopic age data, indicate the Guodian skarn Fe deposit formed contemporaneously with large-scale skarn iron mineralization over the Luxi Block in the Late Mesozoic. The available data demonstrated that the eastern NCC was “destructed” in the Late Mesozoic, as marked by voluminous igneous rocks, faulted-basin formation, high crustal heat flow, and widespread metamorphic core complexes in the eastern part of the NCC. It is thus suggested that the Guodian Fe skarn deposits, together with other deposits of similar ages in the Luxi Block and even in the eastern NCC, were products of this craton destruction. Lithospheric extension and extensive magmatism related to the craton destruction may have provided sufficient heat energy, fluid, chlorine, and Fe for the formation of the Fe deposit.
The Effect of Expanded Graphite Content on the Thermal Properties of Fatty Acid Composite Materials for Thermal Energy Storage
Authors: Zhou Dongyi , Xiao Shuaizhe , Liu Yicai
Published: July 2024

The mass content of expanded graphite (EG) in fatty acid/expanded graphite composite phase-change materials (CPCMs) affects their thermal properties. In this study, a series of capric–myristic acid/expanded graphite CPCMs with different EG mass content (1%, 3%, 5%, 8%, 12%, 16%, and 20%) were prepared. The adsorption performance effect of EG on the PCMs was observed and analyzed. The structure and thermal properties of the prepared CPCMs were characterized via scanning electron microscopy, differential scanning calorimetry, thermal conductivity measurements, and heat energy storage/release experiments. The results show that the minimum mass content of EG in the CPCMs is 7.6%. The phase-change temperature of the CPCMs is close to that of the PCMs, at around 19 °C. The latent heat of phase change is equivalent to that of the PCM at the corresponding mass content, and that of phase change with an EG mass content of 8% is 138.0 J/g. The CPCMs exhibit a large increase in thermal conductivity and a significant decrease in storage/release time as the expanded graphite mass content increases. The thermal conductivity of the CPCM with a mass content of 20% is 418.5% higher than that with a mass content of 5%. With an increase in the EG mass content in CPCMs, the heat transfer mainly transitions from phase-change heat transfer to thermal conductivity.
High Performance Aqueous Zinc-Ion Batteries Developed by PANI Intercalation Strategy and Separator Engineering
Authors: Deng Ling , Sun Kailing , Liu Jie , Li Zeyang , Cao Juexian , Liao Shijun
Published: July 2024

Aqueous zinc-ion batteries (ZIBs) have attracted burgeoning attention and emerged as prospective alternatives for scalable energy storage applications due to their unique merits such as high volumetric capacity, low cost, environmentally friendly, and reliable safety. Nevertheless, current ZIBs still suffer from some thorny issues, including low intrinsic electron conductivity, poor reversibility, zinc anode dendrites, and side reactions. Herein, conductive polyaniline (PANI) is intercalated as a pillar into the hydrated V2O5 (PAVO) to stabilize the structure of the cathode material. Meanwhile, graphene oxide (GO) was modified onto the glass fiber (GF) membrane through simple electrospinning and laser reduction methods to inhibit dendrite growth. As a result, the prepared cells present excellent electrochemical performance with enhanced specific capacity (362 mAh g−1 at 0.1 A g−1), significant rate capability (280 mAh g−1 at 10 A g−1), and admirable cycling stability (74% capacity retention after 4800 cycles at 5 A g−1). These findings provide key insights into the development of high-performance zinc-ion batteries.