bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of Single-Cell RNA Sequencing in Ovarian Development
Authors: Gong Xiaoqin , Zhang Yan , Ai Jihui , Li Kezhen
Published: December 2022

The ovary is a female reproductive organ that plays a key role in fertility and the maintenance of endocrine homeostasis, which is of great importance to women’s health. It is characterized by a high heterogeneity, with different cellular subpopulations primarily containing oocytes, granulosa cells, stromal cells, endothelial cells, vascular smooth muscle cells, and diverse immune cell types. Each has unique and important functions. From the fetal period to old age, the ovary experiences continuous structural and functional changes, with the gene expression of each cell type undergoing dramatic changes. In addition, ovarian development strongly relies on the communication between germ and somatic cells. Compared to traditional bulk RNA sequencing techniques, the single-cell RNA sequencing (scRNA-seq) approach has substantial advantages in analyzing individual cells within an ever-changing and complicated tissue, classifying them into cell types, characterizing single cells, delineating the cellular developmental trajectory, and studying cell-to-cell interactions. In this review, we present single-cell transcriptome mapping of the ovary, summarize the characteristics of the important constituent cells of the ovary and the critical cellular developmental processes, and describe key signaling pathways for cell-to-cell communication in the ovary, as revealed by scRNA-seq. This review will undoubtedly improve our understanding of the characteristics of ovarian cells and development, thus enabling the identification of novel therapeutic targets for ovarian-related diseases.
Combined Transcutaneous Electrical Spinal Cord Stimulation and Task-Specific Rehabilitation Improves Trunk and Sitting Functions in People with Chronic Tetraplegia
Authors: Tharu Niraj Singh , Alam Monzurul , Ling Yan To , Wong Arnold YL , Zheng Yong-Ping
Published: December 2022

The aim of this study was to examine the effects of transcutaneous electrical spinal cord stimulation (TSCS) and conventional task-specific rehabilitation (TSR) on trunk control and sitting stability in people with chronic tetraplegia secondary to a spinal cord injury (SCI). Five individuals with complete cervical (C4–C7) cord injury participated in 24-week therapy that combined TSCS and TSR in the first 12 weeks, followed by TSR alone for another 12 weeks. The TSCS was delivered simultaneously at T11 and L1 spinal levels, at a frequency ranging from 20–30 Hz with 0.1–1.0 ms. pulse width biphasically. Although the neurological prognosis did not manifest after either treatment, the results show that there were significant increases in forward reach distance (10.3 ± 4.5 cm), right lateral reach distance (3.7 ± 1.8 cm), and left lateral reach distance (3.0 ± 0.9 cm) after the combinational treatment (TSCS+TSR). The stimulation also significantly improved the participants’ trunk control and function in sitting. Additionally, the trunk range of motion and the electromyographic response of the trunk muscles were significantly elevated after TSCS+TSR. The TSCS+TSR intervention improved independent trunk control with significantly increased static and dynamic sitting balance, which were maintained throughout the TSR period and the follow-up period, indicating long-term sustainable recovery.
CIGB-300 Anticancer Peptide Differentially Interacts with CK2 Subunits and Regulates Specific Signaling Mediators in a Highly Sensitive Large Cell Lung Carcinoma Cell Model
Authors: Pérez George V. , Rosales Mauro , Ramón Ailyn C. , Rodríguez-Ulloa Arielis , Besada Vladimir , González Luis J. , Aguilar Daylen , Vázquez-Blomquist Dania , Falcón Viviana , Caballero Evelin , Carvalho Paulo C. , Caldeira Rodrigo Soares , Yang Ke , Perera Yasser , Perea Silvio E.
Published: December 2022

Large cell lung carcinoma (LCLC) is one form of NSCLC that spreads more aggressively than some other forms, and it represents an unmet medical need. Here, we investigated for the first time the effect of the anti-CK2 CIGB-300 peptide in NCI-H460 cells as an LCLC model. NCI-H460 cells were highly sensitive toward CIGB-300 cytotoxicity, reaching a peak of apoptosis at 6 h. Moreover, CIGB-300 slightly impaired the cell cycle of NCI-H460 cells. The CIGB-300 interactomics profile revealed in more than 300 proteins that many of them participated in biological processes relevant in cancer. Interrogation of the CK2 subunits targeting by CIGB-300 indicated the higher binding of the peptide to the CK2α′ catalytic subunit by in vivo pull-down assays plus immunoblotting analysis and confocal microscopy. The down-regulation of both phosphorylation and protein levels of the ribonuclear protein S6 (RPS6) was observed 48 h post treatment. Altogether, we have found that NCI-H460 cells are the most CIGB-300-sensitive solid tumor cell line described so far, and also, the findings we provide here uncover novel features linked to CK2 targeting by the CIGB-300 anticancer peptide.
Untargeted Lipidomics Reveals Characteristic Biomarkers in Patients with Ankylosing Spondylitis Disease
Authors: Li Zhengjun , Gu Wanjian , Wang Yingzhuo , Qin Bin , Ji Wei , Wang Zhongqiu , Liu Shijia
Published: December 2022

Objective. Ankylosing spondylitis (AS) is a chronic inflammatory disease of the axial skeleton. Early and accurate diagnosis is necessary for the timely and effective treatment of this disease and its common complications. Lipid metabolites form various kinds of bioactive molecules that regulate the initiation and progression of inflammation. However, there are currently few studies that investigate the alteration of serum lipid in AS patients. Methods. Blood samples were collected from 115 AS patients and 108 healthy controls (HCs). Serum-untargeted lipidomics were performed using ultrahigh-performance liquid chromatography coupled with Q-Exactive spectrometry, and the data were determined by multivariate statistical methods to explore potential lipid biomarkers. Results. Lipid phenotypes associated with disease activity were detected in the serum of patients with AS. Of all 586 identified lipids, there are 297 differential lipid metabolites between the AS and HC groups, of which 15 lipid metabolites are significant. In the AS groups, the levels of triacylglycerol (TAG) (18:0/18:1/20:0) were increased, and the levels of phosphatidylcholine (PC) (16:0e/26:4) and PC (18:1/22:6) were decreased. The areas under the receiver operating characteristic curve (AUC) of TAG (18:0/18:1/20:0), PC (16:0e/26:4), and PC (18:1/22:6) were 0.919, 0.843, and 0.907, respectively. Conclusion. Our findings uncovered that lipid deregulation is a crucial hallmark of AS, thereby providing new insights into the early diagnosis of AS.
Methylenetetrahydrofolate Reductase 677T Allele Is a Risk Factor for Arterial Thrombosis in Chinese Han Patients with Antiphospholipid Syndrome
Authors: Tang Zihan , Shi Hui , Liu Honglei , Cheng Xiaobing , Su Yutong , Ye Junna , Sun Yue , Hu Qiongyi , Chi Huihui , Zhou Zhuochao , Jia Jinchao , Meng Jianfen , Wang Mengyan , Wang Fan , Teng Jialin , Yang Chengde , Liu Tingting
Published: December 2022

Antiphospholipid syndrome (APS) is a systemic autoimmune disorder characterized by the persistent presence of antiphospholipid antibodies (aPL) and thrombotic or obstetric events. Given the heterogeneity of the clinical manifestations, it is likely that genetic and acquired factors are involved in the pathogenesis of APS. The inherited polymorphisms of the thrombophilic gene, including methylenetetrahydrofolate reductase (MTHFR) C677T, type 1 plasminogen activator inhibitor (PAI-1) 4G/5G, factor V Leiden (FVL) G1691A, prothrombin (PT) G20210A, antithrombin (AT), and fibrinogen (Fg) polymorphisms, were analyzed in 67 aPL(+) patients from the Chinese Han population, including 41 APS patients and 26 persistent aPL carriers. The MTHFR C677T genotypes of 105 healthy controls, and the PAI-1 4G/5G polymorphism of 120 healthy controls, from the Chinese Han population were acquired for this study. Both the MTHFR C677T genotype (χ2 = 10.67, p = 0.004) and C/T allele distribution (χ2 = 5.92, p = 0.019) between the aPL(+) patients and healthy controls were found to be significantly different. Furthermore, we observed that the patients with at least one T allele had a higher risk of arterial thrombosis (CT vs. CC, OR 11.00, p= 0.025; CT + TT vs. CC, OR 10.27, p = 0.018). The C677T mutation of MTHFR is a risk factor for arterial thrombosis in Chinese Han patients with APS.
The 2-Oxoglutarate Carrier Is S-Nitrosylated in the Spinal Cord of G93A Mutant hSOD1 Mice Resulting in Disruption of Mitochondrial Glutathione Transport
Authors: Linseman Daniel A. , Winter Aimee N. , Wilkins Heather M.
Published: December 2022

Mitochondrial oxidative stress and dysfunction are strongly implicated in the pathogenesis of amyotrophic lateral sclerosis (ALS). Glutathione (GSH) is an endogenous antioxidant that exists as distinct cytosolic and mitochondrial pools. The status of the mitochondrial GSH pool is reliant on transport from the cytosol through the 2-oxoglutarate carrier (OGC), an inner membrane anion carrier. We have previously reported that the outer mitochondrial membrane protein, Bcl-2, directly binds GSH and is a key regulator of OGC-dependent mitochondrial GSH transport. Here, we show that G93A mutant SOD1 (Cu, Zn-superoxide dismutase) reduces the binding of GSH to Bcl-2 and disrupts mitochondrial GSH uptake in vitro. In the G93A mutant hSOD1 mouse model of ALS, mitochondrial GSH is significantly depleted in spinal cord of end-stage mice. Finally, we show that OGC is heavily S-nitrosylated in the spinal cord of end-stage mice and consequently, the GSH uptake capacity of spinal cord mitochondria isolated from these mutant mice is significantly diminished. Collectively, these findings suggest that spinal cord GSH depletion, particularly at the level of the mitochondria, plays a significant role in ALS pathogenesis induced by mutant SOD1. Furthermore, the depletion of mitochondrial GSH in the G93A mutant hSOD1 mouse model may be caused by the S-nitrosylation of OGC and the capacity of mutant SOD1 to disrupt the Bcl-2/GSH interaction, resulting in a disruption of mitochondrial GSH transport.
A Review of Magnetic Nanoparticle-Based Surface-Enhanced Raman Scattering Substrates for Bioanalysis: Morphology, Function and Detection Application
Authors: Huang Hanbing , Zhang Zhuomin , Li Gongke
Published: December 2022

Surface-enhanced Raman scattering (SERS) is a kind of popular non-destructive and water-free interference analytical technology with fast response, excellent sensitivity and specificity to trace biotargets in biological samples. Recently, many researches have focused on the preparation of various magnetic nanoparticle-based SERS substrates for developing efficient bioanalytical methods, which greatly improved the selectivity and accuracy of the proposed SERS bioassays. There has been a rapid increase in the number of reports about magnetic SERS substrates in the past decade, and the number of related papers and citations have exceeded 500 and 2000, respectively. Moreover, most of the papers published since 2009 have been dedicated to analytical applications. In the paper, the recent advances in magnetic nanoparticle-based SERS substrates for bioanalysis were reviewed in detail based on their various morphologies, such as magnetic core–shell nanoparticles, magnetic core–satellite nanoparticles and non-spherical magnetic nanoparticles and their different functions, such as separation and enrichment, recognition and SERS tags. Moreover, the typical application progress on magnetic nanoparticle-based SERS substrates for bioanalysis of amino acids and protein, DNA and RNA sequences, cancer cells and related tumor biomarkers, etc., was summarized and introduced. Finally, the future trends and prospective for SERS bioanalysis by magnetic nanoparticle-based substrates were proposed based on the systematical study of typical and latest references. It is expected that this review would provide useful information and clues for the researchers with interest in SERS bioanalysis.
Genome Editing in Human Gametes and Embryos: The Legal Dimension in Europe
Authors: Vidalis Takis
Published: December 2022

To date, the legal aspects of the ongoing debate on the application of genome editing in human gametes and early embryos have attracted little attention. In Europe, this seems to have changed with a recent official position that clarifies the meaning of the relevant provision of the common legal instrument on Human Rights and Biomedicine (Oviedo Convention). This provision explicitly prohibits modifications to the genome of future persons and adoptes a precautionary stance with regard to genetic interventions in the human reproductive material. In this article, we examine relevant interpretative options, following the new official clarifications, focusing on the research/clinical application distinctions that characterize their approach. From this viewpoint, we propose an approach that favors basic research activities involving genome editing, even for exploring potential clinical applications under conditions of safety, which may justify a future legislative amendment. Furthermore, we explore the patenting issue, based on the current approach of European case law, and give reasons that may justify patent rights in this ethically sensitive area.
Posterior Reversible Encephalopathy Syndrome after Lenvatinib Therapy in a Patient with Olfactory Neuroblastoma
Authors: Tseng Yu-Ju , Chen Chun-Nan , Hong Ruey-Long , Kung Woon-Man , Huang Abel Po-Hao
Published: December 2022

Posterior reversible encephalopathy syndrome (PRES) is a rare but severe neurological syndrome that may stem from the use of some medications. Although its mechanism is not well-known, hypertension and endothelial dysfunction have been mentioned in previous literature as being related. Lenvatinib serves as a neoplastic agent that inhibits the tyrosine kinase of vascular endothelial growth factor receptors (VEGFR). VEGFR inhibitors result in endothelial dysfunction and consequent hypertension by nitric oxide pathway suppression and endothelin (ET)-1 stimulation. We hypothesized that VEGFR inhibitors would cause PRES. Herein, we report the case of a 40-year-old man with olfactory neuroblastoma who developed PRES while undergoing treatment with lenvatinib, 7 months after initiation. The symptoms included loss of consciousness and seizures. Fortunately, the symptoms and presence of PRES in imaging resolved, 7 days and 1 month, respectively, after cessation of lenvatinib.
Analytic Background in the Neuroscience of the Potential Project “Hippocrates”
Authors: Trofimova Irina
Published: December 2022

This paper reviews the principles identified in analytic neuroscience that could be used in the setup of an international project, “Hippocrates” (H-project), named after the author of the endocrine theory of temperaments. The H-project can aim to summarize the findings in functional neurochemistry of consistent behavioural patterns (CBPs) in health (such as temperament traits) and psychopathology (symptoms of psychiatric disorders); to have systematically structured neurochemical investigations; to have an analysis of CBPs that include all ranges of behavioural histories and to have these modules complemented by regional contrasts related to climate, diets and other bio-environmental factors. The review highlights the benefits of constructivism and illustrates the contrast between constructivism and current approaches in terms of analytic and methodological aspects. (1) “Where” the neurochemical biomarkers should be measured: the review expands the range of needed measurements to out-of-brain systems, including environmental factors, and explores the concept of Specialized Extended Phenotype. (2) “What” should be measured but is missing: the review points to the need for measurement of the “Throw & Catch” neurochemical relays; behavioural and neuronal events contributing to the consistency of the CBPs but not documented in measurements. (3) Structuring the H-project’s setup: the paper briefly describes a proposed earlier neurochemical framework, Functional Ensemble of Temperament that that accommodates the neurochemical continuum between temperament and symptoms of psychiatric disorders. This framework is in line with documented “Throw & Catch” neurochemical relays and can also be used to organize data about the personal and professional history of an individual.
Attentional Bias for Sleep-Related Words as a Function of Severity of Insomnia Symptoms
Authors: Fabbri Marco , Simione Luca , Catalano Laura , Mirolli Marco , Martoni Monica
Published: December 2022

Attentional bias to sleep-related information is thought to be a core feature for developing and/or maintaining insomnia. This study used a hallmark measure of attentional bias, the dot-probe task, to determine whether this bias toward sleep-related stimuli was a function of the severity of insomnia symptoms. A sample of 231 volunteers (175 females; mean age of 26.91 ± 8.05 years) participated in this online study, filling out the Insomnia Severity Index (ISI) and performing a visual dot-probe task. After categorizing individuals based on the ISI score into normal, subclinical, and moderate/severe sleep groups, we only found a marginally significant interaction between sleep groups and the type of stimuli on RTs, suggesting that subclinical and moderate/severe sleep groups reported slower RTs for sleep-related words than for neutral words. When we calculated the attentional bias score (ABS), we found that ABS significantly differed from zero in the moderate/severe sleep group only, suggesting a disengagement for sleep-related information as a function of the severity of insomnia symptoms. This finding seems to suggest that insomnia is related to greater difficulties in shifting away from sleep-related stimuli.
Previous School Bullying-Associated Depression in Chinese College Students: The Mediation of Personality
Authors: Li Hongjie , Qian Xueyan , Gong Jian , Dong Haiying , Chai Xuejiao , Chao Hong , Yang Xiaolei
Published: December 2022

Previous school bullying was associated with increased risk of depression in students. However, little was known about the role of the Big Five personality traits in this association. The purpose of this study was to investigate the possible mediation by the Big Five personality traits in this association in a large group of Chinese college students, and to provide help for educators to prevent students from serious psychological and mental diseases caused by school bullying. Random stratified cluster sampling was used to survey 2152 college students ranging from freshmen to seniors at three universities in Qiqihar city, Heilongjiang Province, China. The risk factors for previous school bullying included gender, living expenses per month, caregivers, parents often quarreling, and divorced parents. Males were more likely to be bullied at school than females. The influencing factors of depression include gender, caregivers, living expenses per month, frequent parents quarreling, and parental divorce. Females were more prone to depression than males. Depression was significantly correlated with all dimensions of school bullying and the Big Five personality traits (p < 0.05). The Big Five personality traits were found to play a significant mediating role between depression and school bullying in up to 45% of cases involving depression. Our major findings highlighted the promising role of personality-based intervention measures in reducing the risk of depression associated with school bullying in Chinese students.
Medical Students’ Perception of Psychotherapy and Predictors for Self-Utilization and Prospective Patient Referrals
Authors: Drury R. Michael , Taylor Nicki , Porter Cheryl
Published: December 2022

The study explored if willingness to seek psychotherapy or refer patients to therapy is predicted by either perception of its usefulness or stigma (public and self-stigma), and if there are any differences based on specific psychological disorders for this population. A cross-sectional study was conducted surveying medical students enrolled at a southeastern university during spring 2022. These medical students completed the Mental Help Seeking Attitudes Scale (MHSAS), revised Self-Stigma of Seeking Help Scale (SSOSH-7), Stigma Scale for Receiving Psychological Help (SSRPH), in addition to vignette-based items assessing the likelihood they would seek therapy treatment and refer a patient for therapy based on two specific psychological disorders. The survey was completed by 106 medical students out of 495 current students (21.4% response rate). The data reveal that the greater the public stigma endorsed, the less likely medical students would be willing to seek therapy for panic disorder; however, the greater the self-stigma, the less likely they would seek therapy for depression. This study found differences in willingness to utilize therapy based on stigma-related attitudes and specific disorders, but not for referring patients.
A Bibliometric Analysis and Review of Nudge Research Using VOSviewer
Authors: Jia Chenjin , Mustafa Hasrina
Published: December 2022

With growing demands of decision making in the current era, the impact of the drivers behind individuals’ preferences and institutional strategies becomes prominent. Coined in 2008, nudge is used to describe incentives for individuals’ choices with foreseeable outcomes but without exclusion of alternative choices or reliance on financial stimuli. Consequently, nudge and its application in real-world situations led to a prosperous surge of studies in multiple disciplines. However, we are still facing a dearth of in-depth understanding of the status quo and future directions of research on nudge in a comprehensive fashion. To address the gap in knowledge, the present study adopted a bibliometric analysis of the existing literature related to the investigation and application of nudge by analyzing 1706 publications retrieved from Web of Science. The results indicated that (a) being a relatively newly developed theory, interest in nudge in academia has expanded both in volume and disciplines, with Western scholars and behavioral economists as the backbones; (b) future studies in nudge-related fields are expected to consolidate its current frontiers in individual behaviors while shedding light on new territories such as the digitalized environment. By incorporating state-of-the-art technologies to investigate extant research, the present study would be pivotal for the holistic understanding of the studies on nudge in recent years. Nevertheless, the inclusiveness and comprehensiveness of the review were limited by the size of the selected literature.
The Impact of Mental Health Predictors of Internet Addiction among Pre-Service Teachers in Ghana
Authors: Essel Harry Barton , Vlachopoulos Dimitrios , Nyadu-Addo Ralph , Tachie-Menson Akosua , Baah Paa Kwame , Owusu-Antwi Charles
Published: December 2022

This study examined the prevalence of addictive Internet behavior and its links with mental health among pre-service teachers in Ghana. A descriptive, correlational design was employed with 405 pre-service teachers from colleges of education and a public university in Ghana participating in this study. The sample completed a sociodemographic survey about loneliness, life satisfaction, depression, self-esteem, and the Internet addiction scales (abridged form). The results revealed that there was a significant relationship between pre-service teachers’ Internet addiction, depression, life satisfaction, and loneliness; however, depression was the least influential factor in addictive Internet use. Additionally, there was a statistically significant nexus between self-esteem, loneliness, depression, and life satisfaction. In addition, all the above-mentioned variables were discovered to explain 56.3% of the absolute variance in addiction to the Internet. Among the variables linked with Internet addiction and its dimensions, loneliness appeared to be the most significant. Institutional coping programs with Internet addiction should be established within the scope of the university administration, supporting pre-service teachers’ mental health. Finally, the development of awareness campaigns on the menaces associated with Internet usage and mental health through extracurricular programs is recommended.
The Relationship between Face Processing, Cognitive and Affective Empathy
Authors: Moret-Tatay Carmen , Mundi-Ricós Paloma , Irigaray Tatiana Quarti
Published: December 2022

This study aims to examine the relationship between affective and cognitive empathy scores and perceptual face recognition skills. A total of 18 young adults participated in the study. Cognitive and Affective Empathy Test (TECA), The eyes Test and an experimental task were carried out. The experimental task has two blocks, a presentation, and a recognition phase, under the Karolinska battery of images expressing different emotions. Cognitive empathy sub-factors were found to be related to the hit rate on the recognition of surprise faces as well as the discarding of faces of disgust. In relation to the hit rate on discarding faces of disgust, this was related to perspective taking. Reaction time and Cognitive empathy subfactors were found to be positively correlated to the recognition of disgust, surprise, and sadness. Lastly, Perspective taking was also related to the discarding of disgust reaction time in a direct way. The relationships between affective empathy and other measures for emotional face recognition were not statistically significant. Knowledge of individual differences in cognitive and affective empathy, as well as of their relationship with behavioral responses such as the recognition or dismissal of emotional faces are of interest for social interaction and in psychotherapy.
Relationship between the Engel Coefficient, Life Satisfaction, and Subjective Health for Senior Citizens in Korea: Moderating Effect of COVID-19
Authors: Moon Joonho , Hwang Jinsoo , Lee Won Seok
Published: December 2022

The purpose of this study was to explore the determinants of life satisfaction for Korean senior citizens. Subjective health and the Engel coefficient were used as the explanatory variables. This research also aimed to examine the moderating effect of Coronavirus disease 2019 (COVID-19) on the relationship between the Engel coefficient and life satisfaction for Korean senior citizens. Archival data (Korean longitudinal study of aging) were used for this work. The study period is 2018 and 2020; the number of observations was 3879. Multiple linear regression analysis was performed to test the research hypotheses. To examine further, this research performed additional analyses: sub-sample analysis, median split, and independence tests. The results indicated that the Engel coefficient is negatively associated with life satisfaction, while subjective health is positively related to life satisfaction. Moreover, Korean senior citizens’ life satisfaction was higher during the COVID-19 pandemic. This research provides information for building policy for senior Korean citizens.
Public Health Safety in Community Living Circles Based on a Behavioral Motivation Perspective: Theoretical Framework and Evaluation System
Authors: Zhong Qikang , Li Bo , Chen Yue , Zhu Jiawei
Published: December 2022

Public health problems, such as the spread of COVID-19 and chronic diseases, are mainly caused by the daily life activities of community residents. Therefore, there is a need to build a healthy and safe community living circle through the evaluation of health behaviors in daily life. This paper proposes a theoretical framework and evaluation system for public health safety in community living circles, from a behavioral motivation perspective. Firstly, based on the behavioral motivation theory, a theoretical framework for the study of public health safety in community living circles is constructed from the perspective of the “project–activity–health” coupling relationship network, regarding community residents’ daily life activities. Then, a public health safety evaluation system for community living circles is proposed based on this framework, which includes the following: (1) identifying the scope of community living circles based on Spatio-temporal Activities Analysis; (2) Based on the theory of protection motivation, a health behavior evaluation model based on the three elements of “spatial and temporal geographical environment–daily life activities–public health safety” is established; (3) Based on the hierarchy of public health problems, a public health safety evaluation model of the community living circle is established. The behavioral motivation-based evaluation system explores a new approach and research paradigm for community-scale public health safety theory; this will help to achieve the goal of “healthy communities” when further empirical evidence is available.
Structural and Spatial Minimal Requirement Efficacy of Emergency Shelters for Different Emergencies
Authors: Beatini Valentina , Rajanayagam Heshachanaa , Poologanathan Keerthan
Published: December 2022

Natural and human-induced disasters have become more frequent in recent years, and this has increased the need for effective, high-quality, quick, easy-to-assemble, and affordable emergency housing solutions. The purpose of this study is to create a knowledge base for researchers and developers working in the structural and structural-related fields to favour the development of relevant and most appropriate assistance for emergency housing that could meet the anticipated future rising demands. The focus of the research is emergency shelters for the Global South, an area of research sparsely addressed within the structural-related field. The emergency sheltering process has so many variabilities in its duration and unfolding that many agencies suggest relying on the resilience of those in need. This can have dramatic human repercussions and eventually further burden natural resources. To reach its goal, the paper shifts the attention to information from field actors and global agencies and employs a multiple case studies approach, conducted through a grounded theory methodology. The process has allowed identification of a list of structural-related issues faced by users, acting as codes in the grounded theory methodology, the associated challenges for authorities in addressing them, acting as categories, and some ideal solutions, derived from the theoretical coding. The research concludes that the challenges of the sheltering process shall be read through sustainability housing indicators and that the constraints of the former may be stimuli to the application of innovative and more inclusive procedures within the latter. The study fosters a new theoretical approach in post-disaster housing, which encourages more interdisciplinary collaborations and empirical investigations that will potentially enhance post-disaster housing sustainability and facilitate the development of emergency shelter construction schemes.
Fire Resistance Test and Numerical Simulation on the Tube Structure of Steel–Concrete–Steel Immersed Tube Tunnel
Authors: Li Jing , Cao Peng , Jiang Shuping , Zhang Dandan
Published: December 2022

To provide references for the fire prevention design of steel–concrete–steel immersed tube tunnels, four types of test conditions—no fire protection, fireproof coating insulation, single-layer seam fireproof boards, and double-layer seam fireproof boards—were carried out using partial full-size structural test members. Additionally, the thermal insulation effects of various fireproofing technology solutions were contrasted and analyzed. Combined with the numerical simulation analysis, the temperature distribution law inside the tube structure under various fireproofing measures and the temperature rise law of measuring points at different depths were studied, and the protective effect of the fireproof layers on the tube structure under high fire temperature was demonstrated. The results of the numerical simulation and the experimental data agree well. The results show that adding fireproof layers can significantly lower both the steel shell’s surface temperature and the depth of fire impact. Without fire protection, the surface temperature of the bottom steel shell exceeds 300 °C at 69 s, and the member bursts. The fireproof coatings are cracked and flaking and cannot meet the fire resistance limits. Both single-seam and double-seam schemes of calcium silicate boards can meet the fire resistance limit requirements and the latter has a better heat insulation effect.
Development of a Classification Framework for Construction Personnel’s Safety Behavior Based on Machine Learning
Authors: Yin Shiyi , Wu Yaoping , Shen Yuzhong , Rowlinson Steve
Published: December 2022

Different sets of drivers underlie different safety behaviors, and uncovering such complex patterns helps formulate targeted measures to cultivate safety behaviors. Machine learning can explore such complex patterns among safety behavioral data. This paper aims to develop a classification framework for construction personnel’s safety behaviors with machine learning algorithms, including logistics regression (LR), support vector machine (SVM), random forest (RF), and categorical boosting (CatBoost). The classification framework has three steps, i.e., data collection and preprocessing, modeling and algorithm implementation, and optimal model acquisition. For illustrative purposes, five common safety behaviors of a random sample of Hong Kong-based construction personnel are used to validate the classification framework. To achieve high classification performance, this paper employed a combinative strategy, consisting of feature selection, synthetic minority over-sampling technique (SMOTE), one-hot encoding, standard scaler and classifiers to classify safety behaviors, and multi-objective slime mould algorithm (MOSMA) to optimize parameters in the classifiers. Results suggest that the combinative strategy of CatBoost–MOSMA achieves the highest classification performance with the maximum average scores, including area under the curve of receiver characteristic operator (AUC) ranging from 0.84 to 0.92, accuracy ranging from 0.80 to 0.86, and F1-score ranging from 0.79 to 0.86. From the optimal model, a unique set of important features was identified for each safety behavior, and ten out of the 46 input indicators were found important for all five safety behaviors. Based on the findings, this study advocates using the machine learning strategy of CatBoost–MOSMA in future construction safety behavior research and makes concrete and targeted suggestions to cultivate different construction safety behaviors.
A Close Examination of Ankara’s Reinforced Concrete Buildings Designed and Constructed between 1923 and 1938
Authors: Tunc Gokhan , Tunc Tanfer Emin
Published: December 2022

The Republic of Turkey was established in 1923 out of the remains of the Ottoman Empire. Between 1923 and 1938, the Turkish republic underwent fifteen years of rapid expansion and growth, with Ankara as its new capital and Mustafa Kemal Atatürk (1881–1938) as its first president. During this period, reinforced concrete (RC) played a significant role in the construction of Ankara’s public-use buildings. This study focuses on 57 of these structures, built either partially, or entirely, out of RC. The buildings are classified with respect to their duration, soil properties, foundation types, structural design details, construction types, materials and overall costs. In order to provide a better picture of the time period in which these buildings were designed and constructed, the technical, financial and political aspects of the projects, and the difficulties and challenges involved in their design and construction, are also discussed. Furthermore, this study outlines the impact of foreign engineers, construction workers, firms and the educational system on the development of civil engineering and use of RC in Turkey.
Monitoring of Wind Effects on a Super-Tall Building under a Typhoon
Authors: Pan Haoran , Wu Jiurong , Fu Jiyang
Published: December 2022

Field measurements are critical to further understand the structural behavior of super-tall buildings under strong wind actions. This paper presents field measurements that reflect the wind characteristics and wind effects on Leatop Plaza under Typhoon Vicente. Wind field characteristics, including the turbulence intensity, gust factor, and power spectral density of wind speed in an urban area, were obtained on the basis of a statistical analysis of measured wind data. Subsequently, measured wind-induced accelerations were used to evaluate the dynamic characteristics of the building and the effects of wind on it. On the basis of the first several modes, the modal properties, i.e., the natural frequency and damping ratio, were identified via the fast Bayesian fast Fourier transform method and compared with those identified using the stochastic subspace method. The discrepancy between the identified results and finite element model predictions is presented and discussed. Finally, the variation in the modal parameters with respect to time and the vibration amplitude was analyzed while considering the associated posterior uncertainty.
Review on the Influence and Control of Sulfur-Containing Tailings on the Strength of Cemented Backfill in Metal Mines
Authors: Wang Jinxing , Xing Menghang , Yang Xiaolin , Jiao Huazhe , Chen Fengbin , Yang Liuhua , Yu Jianxin , Fu Yu
Published: December 2022

With the vigorous development of the world’s mineral resources, the global ecological environment has been severely damaged. The tailings cemented filling technology is an important way to realize the green and low-carbon development of the mining industry. However, sulfur-containing tailings from metal mines can destroy the stability and strength of cemented tailings backfill. Therefore, it is imperative to reduce the harm of the sulfur-containing tailings to the strength of cemented tailings backfill. Firstly, based on the research results of sulfur-containing tailings cemented backfill in recent years, this paper reviews the influence of sulfur-containing tailings on the strength of cemented backfill. Accordingly, the mechanism of strength failure of cemented backfill caused by sulfur-containing tailings is further studied, and the erosion failure of sulfide and sulfate is deeply discussed and analyzed. In addition, three control measures are proposed, including adjusting the combination of filling materials and optimizing the filling ratio, controlling the oxidation conditions in the filling process, and adding ad-mixtures as a supplement. Finally, the main conclusions and outlooks of this review are summarized. The purpose of this review is to provide guidance to improve the strength and durability of the cemented sulfur tailings backfill, effectively treating metal tailings, and to propose some ideas for the further improvement and development of the tailings cemented filling technology.
Gaussian Process Regression for Seismic Fragility Assessment: Application to Non-Engineered Residential Buildings in Indonesia
Authors: Sarli Prasanti Widyasih , Palar Pramudita Satria , Azhari Yuni , Setiawan Andri , Sanjaya Yongky , Sharon Sophia C. , Imran Iswandi
Published: December 2022

Indonesia is located in a high-seismic-risk region with a significant number of non-engineered houses, which typically have a higher risk during earthquakes. Due to the wide variety of differences even among parameters within one building typology, it is difficult to capture the total risk of the population, as the typical structural engineering approach to understanding fragility involves tedious numerical modeling of individual buildings—which is computationally costly for a large population of buildings. This study uses a statistical learning technique based on Gaussian Process Regression (GPR) to build the family of fragility curves. The current research takes the column height and side length as the input variables, in which a linear analysis is used to calculate the failure probability. The GPR is then utilized to predict the fragility curve and the probability of collapse, given the data evaluated at the finite set of experimental design. The result shows that GPR can predict the fragility curve and the probability of collapse well, efficiently allowing rapid estimation of the population fragility curve and an individual prediction for a single building configuration. Most importantly, GPR also provides the uncertainty band associated with the prediction of the fragility curve, which is crucial information for real-world analysis.
Pharmacokinetic Basis for Using Saliva Matrine Concentrations as a Clinical Compliance Monitoring in Antitumor B Chemoprevention Trials in Humans
Authors: Bui Dinh , McWilliams Lenora A. , Wu Lei , Zhou Haiying , Wong Stuart J. , You Ming , Chow Diana S.-L. , Singh Rashim , Hu Ming
Published: December 2022

This study reports the first clinical evidence of significantly high secretion of matrine in a multi-component botanical (Antitumor B, ATB) into human saliva from the systemic circulation. This is of high clinical significance as matrine can be used as a monitoring tool during longitudinal clinical studies to overcome the key limitation of poor patient compliance often reported in cancer chemoprevention trials. Both matrine and dictamine were detected in the saliva and plasma samples but only matrine was quantifiable after the oral administration of ATB tablets (2400 mg) in 8 healthy volunteers. A significantly high saliva/plasma ratios for Cmax (6.5 ± 2.0) and AUC0–24 (4.8 ± 2.0) of matrine suggested an active secretion in saliva probably due to entero-salivary recycling as evident from the long half-lives (t1/2 plasma = 10.0 ± 2.8 h, t1/2 saliva = 13.4 ± 6.9 h). The correlation between saliva and plasma levels of matrine was established using a population compartmental pharmacokinetic co-model. Moreover, a species-relevant PBPK model was developed to adequately describe the pharmacokinetic profiles of matrine in mouse, rat, and human. In conclusion, matrine saliva concentrations can be used as an excellent marker compound for mechanistic studies of active secretion of drugs from plasma to saliva as well as monitor the patient’s compliance to the treatment regimen in upcoming clinical trials of ATB.
Prospective Assessment of Tumour Burden and Bone Disease in Plasma Cell Dyscrasias Using DW-MRI and Exploratory Bone Biomarkers
Authors: Agarwal Gaurav , Nador Guido , Varghese Sherin , Getu Hiwot , Palmer Charlotte , Watson Edmund , Pereira Claudio , Sallemi Germana , Partington Karen , Patel Neel , Soundarajan Rajkumar , Mills Rebecca , Brouwer Richard , Maritati Marina , Shah Aarti , Peppercorn Delia , Oppermann Udo , Edwards Claire M. , Rodgers Christopher T. , Javaid Muhammad Kassim , Gooding Sarah , Ramasamy Karthik
Published: December 2022

Novel biomarkers for tumour burden and bone disease are required to guide clinical management of plasma cell dyscrasias. Recently, bone turnover markers (BTMs) and Diffusion-Weighted Magnetic Resonance Imaging (DW-MRI) have been explored, although their role in the prospective assessment of multiple myeloma (MM) and monoclonal gammopathy of undetermined significance (MGUS) is unclear. Here, we conducted a pilot observational cohort feasibility study combining serum BTMs and DW-MRI in addition to standard clinical assessment. Fifty-five patients were recruited (14 MGUS, 15 smouldering MM, 14 new MM and 12 relapsed MM) and had DW-MRI and serum biomarkers (P1NP, CTX-1, ALP, DKK1, sclerostin, RANKL:OPG and BCMA) measured at baseline and 6-month follow-up. Serum sclerostin positively correlated with bone mineral density (r = 0.40−0.54). At baseline, serum BCMA correlated with serum paraprotein (r = 0.42) and serum DKK1 correlated with serum free light chains (r = 0.67); the longitudinal change in both biomarkers differed between International Myeloma Working Group (IMWG)-defined responders and non-responders. Myeloma Response Assessment and Diagnosis System (MY-RADS) scoring of serial DW-MRI correlated with conventional IMWG response criteria for measuring longitudinal changes in tumour burden. Overall, our pilot study suggests candidate radiological and serum biomarkers of tumour burden and bone loss in MM/MGUS, which warrant further exploration in larger cohorts to validate the findings and to better understand their clinical utility.
Potential Impact of Preoperative Circulating Biomarkers on Individual Escalating/de-Escalating Strategies in Early Breast Cancer
Authors: Gianni Caterina , Palleschi Michela , Merloni Filippo , Bleve Sara , Casadei Chiara , Sirico Marianna , Di Menna Giandomenico , Sarti Samanta , Cecconetto Lorenzo , Mariotti Marita , De Giorgi Ugo
Published: December 2022

The research on non-invasive circulating biomarkers to guide clinical decision is in wide expansion, including the earliest disease settings. Several new intensification/de-intensification strategies are approaching clinical practice, personalizing the treatment for each patient. Moreover, liquid biopsy is revealing its potential with multiple techniques and studies available on circulating biomarkers in the preoperative phase. Inflammatory circulating cells, circulating tumor cells (CTCs), cell-free DNA (cfDNA), circulating tumor DNA (ctDNA), and other biological biomarkers are improving the armamentarium for treatment selection. Defining the escalation and de-escalation of treatments is a mainstay of personalized medicine in early breast cancer. In this review, we delineate the studies investigating the possible application of these non-invasive tools to give a more enlightened approach to escalating/de-escalating strategies in early breast cancer.
Current Status of Research on Small Extracellular Vesicles for the Diagnosis and Treatment of Urological Tumors
Authors: Zhang Mengting , Lu Yukang , Wang Lanfeng , Mao Yiping , Hu Xinyi , Chen Zhiping
Published: December 2022

Extracellular vesicles (EVs) are important mediators of communication between tumor cells and normal cells. These vesicles are rich in a variety of contents such as RNA, DNA, and proteins, and can be involved in angiogenesis, epithelial-mesenchymal transition, the formation of pre-metastatic ecological niches, and the regulation of the tumor microenvironment. Small extracellular vesicles (sEVs) are a type of EVs. Currently, the main treatments for urological tumors are surgery, radiotherapy, and targeted therapy. However, urological tumors are difficult to diagnose and treat due to their high metastatic rate, tendency to develop drug resistance, and the low sensitivity of liquid biopsies. Numerous studies have shown that sEVs offer novel therapeutic options for tumor treatment, such as tumor vaccines and tumor drug carriers. sEVs have attracted a great deal of attention owing to their contribution to in intercellular communication, and as novel biomarkers, and role in the treatment of urological tumors. This article reviews the research and applications of sEVs in the diagnosis and treatment of urological tumors.