bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing the Effects of Surface Conditions on Potential Evapotranspiration in a Humid Subtropical Region of China
Authors: Wang Hailong , Zheng Jinghua
Published: February 2022

The ability of the atmosphere to remove water from land surface is measured by potential evapotranspiration (ETp), which is often inferred by the reference evapotranspiration (ETo). Therefore, ETp is often considered only influenced by the above-ground meteorological conditions. Based on its concept, ETp should also link with surface conditions that influence the surface resistance. Such linkages differ in dry and wet regions with different surface covers. Here, we calculated ETo and especially analyzed the effects of surface conditions including vegetation cover indicated by NDVI (Normalized Difference Vegetation Index) and root-zone SWC (soil water content) in a humid subtropical province of China. Results show that ETp, NDVI, wind speed, temperatures have increased significantly during 1982–2015 and relative humidity (RH) has decreased significantly. Linear trends of these variables varied across seasons, but similarities were found between spring and winter and between summer and autumn. Summer saw the greatest changes in ETp per unit of environmental variable change. Solar radiation, RH, and precipitation exerted overall stronger influence on ETp (R2 > 0.50) than other factors. NDVI and SWC were found positively and negatively affecting ETp at all time scales. Partial correlation analysis showed significant influence of NDVI and SWC at the monthly scale; moreover, SWC influenced ETp more significantly in summer than other seasons (p < 0.05). Since actual evapotranspiration is often deducted from ETp by multiplicative stress functions in many hydrologic models, understanding the relationships between ETp and environmental changes can help improve the formulation and estimation of actual evapotranspiration.
Gender in the World Food Economy: Inequitable Transformation of Haiti’s Food Economy
Authors: Vansteenkiste Jennifer
Published: February 2022

Over the past five decades, agricultural trade liberalization and integration into the global food system have undermined gender equality, women’s empowerment, and food security in Haiti. International actors who design agricultural policies are aware of gendered contributions to Haitian food security, yet ignore this knowledge to privilege an ideological belief that trade liberalization and structural adjustment programs will advance gender equality. I argue that agriculture and food policies must treat gender roles and responsibilities as constitutive to the solution by recognizing their critical contribution to the structure of Haitian agrarian society and accordingly food security. I advance this claim by analyzing scholarly literature, institutional documents, and interviews with interlocutors (n= 292) from rural, peri-urban, and urban settings across Haiti to document how: 1) dominant narratives support an ideological belief that trade liberalization will advance gender equality; 2) integration of Haiti’s local food economy into the world food economy demands the reorientation of gendered labour; 3) women must participate in a system that while purporting to improve food security, ultimately diminishes their nutrition and social, economic and political wellbeing. This research documents that policies designed to globalize Haiti’s food economy on the premise of improving food security actually force women to abandon agricultural production and intensify their labour in less lucrative distribution and consumption roles of imported goods. This analysis documents that the transition ultimately reifies gender inequalities, heighten food insecurity, and contributes to feminist food scholarship.
Bushpig (Potamochoerus larvatus) Hunting in Rural Areas of Madagascar and Its Health and Socioeconomic Implications
Authors: Rakotoarivony Rianja , Molia Sophie , Rakotomalala Eric , Ramy-Ratiarison Ranto , Jori Ferran , Pedrono Miguel
Published: February 2022

Bushmeat consumption and trade plays a relevant role in many tropical countries as a source of protein and income for rural populations. In Madagascar, rural populations depend heavily on natural resources and wildlife as source of income and protein. The bushpig (Potamochoerus larvatus) is the largest mammal available in the island and regularly hunted. However, little is known about the importance and characteristics of this activity and its implication as a potential source of pathogens for both humans and domestic animals. A cross-sectional study was conducted in 2014–2015 in five different regions of rural Madagascar suspected to have significant bushpig populations to (i) quantify and characterize the importance of bushpig hunting, (ii) assess the socioeconomic impact of bushpig trade, (iii) evaluate the potential pathogen transmission between bushpigs, domestic pigs and humans. A total of 77 hunters, 10 butchers and 95 pig farmers were individually interviewed. Hunting seasonality and the perception of local hunters with regards to the dynamics of bushpig populations in the last decade differed between the tropical dry and tropical sub-arid climatic zones. The top reason for hunting bushpigs was crop protection but personal consumption and selling of meat were also common. Hunting efficacy was largely dependent on the technique used. Snares and traps, the most widely used techniques, allowed the majority of hunters to catch from one to 10 bushpigs per year. Limited commercial bushpig trade was observed with only 0.8 bushpig sold in average per year and per hunter, representing a 16 USD income. The average price per kilo sold was USD 0.8 and the average profit received by each butcher/collector after the sale of a carcass was USD 11.9. No perception of disease risks nor precautions were taken to prevent potential pathogen transmission from bushpig to humans or pigs. Most of the hunters (68%) indicated that they had never seen a diseased bushpig. Bushpig hunting in our study areas in Madagascar was basically a small-scale subsistence hunting, very different from commercial bushmeat hunting described in areas of Central Africa or the Amazon Basin. More research is needed to verify the sustainability of bushpig hunting and its potential role in terms of reducing pressure on other endemic wildlife species and transmitting pathogens to humans and pigs.
Segmental Tissue Speckle Tracking Predicts the Stenosis Severity in Patients With Coronary Artery Disease
Authors: Chaichuum Srisakul , Chiang Shuo-Ju , Daimon Masao , Chang Su-Chen , Chan Chih-Lin , Hsu Chu-Ying , Chen Hsiang-Ho , Tseng Ching-Li
Published: February 2022

Objective

Two-dimensional speckle tracking echocardiography (2D-STE) has been used as a diagnostic tool for coronary artery disease (CAD). However, whether vessel supplied myocardial strain and strain rate (SR) predict the severity of coronary artery stenosis in patients with CAD is unknown. This study aimed to investigate correlation of cardiac mechanical parameters in tissue speckle tracking measurements with coronary artery stenosis diagnosed by cardiac catheterization in patients with clinically diagnosed CAD.

Methods and Results

Among 59 patients analyzed, 170 vessels were evaluated by coronary angiography and the corresponding echocardiography to quantify left ventricular myocardial strain and SR. The average longitudinal strain and SR of the segmental myocardium supplied by each coronary artery were calculated to achieve vessel myocardium strain (VMS) and strain rate (VMSR). The VMS and VMSR at each of four severity levels of stenosis showed significant differences among groups (p = 0.016, and p < 0.001, respectively). The strain and SR in vessels with very severe stenosis (≥75%, group IV; n = 29), 13.9 ± 4.3, and 0.9 ± 0.3, respectively, were significantly smaller than those of vessels with mild stenosis ≤ 25%, group I; n = 88, 16.9 ± 4.9, p = 0.023, and 1.2 ± 0.3, p = 0.001, respectively. The SR in vessels with moderate stenosis (26–49%, group II; n = 37), 1.0 ± 0.2, was significantly smaller than that in vessels with mild stenosis vessels (p = 0.021). The lower VMS and VMSR, the higher possibility of severe coronary stenosis is. The VMS and VMSR lower than 13.9 ± 4.3 and 0.9 ± 0.3, respectively predicted the severe coronary stenosis. The VMS and VMSR higher than 16.9 ± 4.9 and 1.2 ± 0.3, respectively predicted mild or no coronary artery stenosis.

Conclusions

The actual stenosis rate in catheterization demonstrates that this technique was able to assess coronary artery condition. Thus, the application of a non-invasive method of 2D-STE to evaluate and simplify diagnosis of CAD is feasible.
Investigation About Variation Law of Frost Heave Force of Seasonal Cold Region Tunnels: A Case Study
Authors: Zhang Yuwei , Fan Shengyuan , Yang Donghui , Zhou Fei
Published: February 2022

The frost heave force (FHF) is one of the most important reason of inducing freezing damages of tunnel in seasonal frozen regions. Analyzing the change law of FHF is helpful to design for avoiding freezing damages in the tunnel lining. Field measurement is an approved method used in mechanical analysis of tunnel lining, however, it is difficult to measure the FHF directly, which is changed coupled with the original surrounding rock pressure (OSRP). In this paper, in order to clarifying the change law of the FHF, a new measuring method was presented. The best advantage of this method is that the FHF could be separated from the OSRP by measuring the surrounding rock temperature and the lining pressure at the same time. A lot of field measurement work about the FHF was conducted based on Jichoushan tunnel and the change law of the FHF was analyzed using the presented method. The results can provide preference for the antifreeze design of tunnel in seasonal cold regions.
New Discovery of Oligocene Strata in the Topernawi Formation, Turkana County, Kenya
Authors: Sousa Francis J. , Cox Stephen E. , Hemming Sidney R. , Rasbury E. Troy , Steponaitis Elena , Hatton Kevin , Saslaw Mae , Henkes Gregory , Princehouse Patricia , Vitek Natasha S. , Nengo Isaiah
Published: February 2022

New field observations and 40Ar/39Ar geochronology reveal that the Topernawi Formation of the Ekitale Basin, northern Turkana Depression, Turkana County, Kenya was deposited entirely during the Oligocene between 29.7 ± 0.5 Ma and 29.24 ± 0.08 Ma. These bracketing ages are determined via new 40Ar/39Ar geochronology on a basaltic lava flow at the base of the section and a felsic ignimbrite near the top. A newly discovered basal unit and interbedded lava flow result in a new total sedimentary thickness of 92 m. The Topernawi Formation is the oldest dated syn-rift sedimentary section in the northern Turkana Depression.
Reducing Math Anxiety in School Children: A Systematic Review of Intervention Research
Authors: Balt Miriam , Börnert-Ringleb Moritz , Orbach Lars
Published: February 2022

Recent studies indicate that math anxiety (MA) can already be found in school-aged children. As early MA depicts a potential risk for developing severe mathematical difficulties and impede the socio-emotional development of children, distinct knowledge about how to reduce MA in school-aged children is of particular importance. Therefore, the goal of this systematic review is to summarize the existing body of research on MA interventions for children by identifying the approaches, designs, and characteristics as well as the effects of the interventions.
Hormonal Replacement Treatment for Frozen-Thawed Embryo Transfer With or Without GnRH Agonist Pretreatment: A Retrospective Cohort Study Stratified by Times of Embryo Implantation Failures
Authors: Xia Leizhen , Tian Lifeng , Zhang Shanshan , Huang Jialyu , Wu Qiongfang
Published: February 2022

Objective

To evaluate the efficacy of the long-acting gonadotropin-releasing hormone agonist (GnRH-a) administration before hormone replacement treatment for frozen-thawed embryo transfer in women with different times of embryo implantation failures.

Methods

A retrospective cohort study was performed between January 2015 and December 2019. A total of 9263 women who underwent frozen-thawed embryo transfer were included in the study. The study is divided into three parts based on the times of embryo implantation failures. The sample sizes were 4611 for no implantation failure, 3565 for one failure and 1087 for multiple failures. Two endometrium preparation protocols, HRT and HRT with GnRH-a pretreatment (G-HRT), were compared. Confounding factors were treated by propensity score matching and generalized estimation equation.

Results

For women with no failure of embryo implantation, the live birth rate was not statistically different when they underwent HRT and G-HRT (HRT: 42.75% [498/1165], G-HRT: 45.24% [527/1165], P=0.2261). Similar outcome also appeared in women with one failure of embryo implantation (HRT: 47.22% [535/1133], G-HRT: 50.31% [570/1131], P=0.1413). For women with multiple failures of embryo implantation, the live birth rate was significantly difference (HRT: 38.74% [117/302], G-HRT: 45.48% [357/785], P=0.0449). When stratified by age, the live birth rate is similar for women older than 37 years. Generalized estimation equation showed that GnRH agonist pretreatment was independently associated with the live birth rate for women with multiple failures (adjust OR: 1.5, 95%CI: [1.12-2.00]).

Conclusion

For women with no/one failure of embryo implantation, the live birth rate is similar between HRT and G-HRT protocols. For women with multiple failure of embryo implantation, GnRH agonist pretreatment is beneficial to raise the live birth rate.
Value of Contrast-Enhanced Ultrasound for Evaluation of Cervical Lymph Node Metastasis in Papillary Thyroid Carcinoma
Authors: Fang Fengkai , Gong Yi , Liao Liyan , Ye Fei , Zuo Zhongkun , Li Xiaodu , Zhang Qi , Tang Kui , Xu Yan , Zhang Rongsen , Chen Sijie , Niu Chengcheng
Published: February 2022

The aim of the study was to evaluate the diagnostic value of contrast-enhanced ultrasound (CEUS) in distinguishing between benign and malignant cervical lymph nodes (LNs) in patients with papillary thyroid carcinoma (PTC). Two hundred and one cervical LNs (157 metastatic from PTC and 44 benign) were evaluated using conventional ultrasonography (US) and CEUS before biopsy or surgery. Histopathology was used as the gold standard. We evaluated the size, long axis/short axis ratio (L/S), fatty hilum, hyper-echogenicity, calcification, cystic change, peripheral vascularity and CEUS parameters for each lymph nodule. The CEUS parameters included enhancement type, homogeneity, perfusion type, ring enhancement, peak intensity (PI) index and area under the curve (AUC) index. Univariate analysis demonstrated that compared with benign LNs, malignant LNs more frequently had L/S < 2, absence of a fatty hilum, presence of hyper-echogenicity, presence of calcification, peripheral vascularity, hyper-enhancement, heterogeneous enhancement, centripetal perfusion, ring enhancement, PI index > 1 and AUC index > 1 on preoperative US and CEUS. Binary logistic regression analysis demonstrated that hyper-enhancement, centripetal perfusion, and ring enhancement are independent CEUS characteristics related to malignant LNs for their differentiation from benign LNs (all p < 0.05). Our study indicated that preoperative CEUS characteristics may serve as a useful tool to identify malignant cervical LNs from benign cervical LNs.
Image Analysis for Rapid Assessment and Quality-Based Sorting of Corn Stover
Authors: Ding Ling , Hoover Amber N. , Emerson Rachel M. , Lin Kuan-Ting , Gruber Josephine N. , Donohoe Bryon S. , Klinger Jordan L. , Colby Rachel D. , Thomas Brad J. , Smith William A. , Ray Allison E.
Published: February 2022

Imaging in the visible spectrum is a low-cost tool that can be readily deployed for in-field or over-belt monitoring of biomass quality for bio-refining operations. Rapid image analysis coupled with innovative preprocessing may reduce the impacts of feedstock variability through identification of contaminants or other material attributes to guide selective sorting and quality management. Image analysis was employed to evaluate the quality of corn stover in red-green-blue (RGB) chromatic space. This study used controlled, bench-scale imaging as a proof-of-concept for rapid quality assessment of corn stover based on variations in material attributes, including chemical and physical attributes, that relate to biological degradation and soil contamination. Logistic regression-based classification algorithms were used to develop a method for biomass screening as a function of biological degradation or soil contamination. This study demonstrated the use of image analysis to extract features from RGB color space to investigate variations in critical material attributes from chemical composition of corn stover. Fourier transform infrared (FT-IR) suggested a correlation between red band intensity and biological degradation, while detailed surface texture analysis was found to distinguish among variations in ash. These insights offer promise for development of a rapid screening tool that could be deployed by farmers for in-field assessment of biomass quality or biorefinery operators for in-line sorting and process optimization.
Identification, Molecular Characteristics, and Evolution of GRF Gene Family in Foxtail Millet (Setaria italica L.)
Authors: Chen Huilong , Ge Weina
Published: February 2022

Growth-regulating factor (GRF) is a multigene family that plays a vital role in the growth and development of plants. In the past, the GRF family of many plants has been studied. However, there is not a report about identification and evolution of GRF in foxtail millet (Setaria italia). Here, we identified 10 GRF genes in foxtail millet. Seven (70.00%) were regulated by Sit-miR396, and there were 19 optimal codons in GRFs of foxtail millet. Additionally, we found that WGD or segmental duplication have affected GRFs in foxtail millet between 15.07 and 45.97 million years ago. Regarding the GRF gene family of land plants, we identified a total of 157 GRF genes in 15 representative land plants. We found that GRF gene family originated from Group E, and the GRF gene family in monocots was gradually shrinking. Also, more loss resulted from the small number of GRF genes in lower plants. Exploring the evolution of GRF and functional analysis in the foxtail millet help us to understand GRF better and make a further study about the mechanism of GRF. These results provide a basis for the genetic improvement of foxtail millet and indicate an improvement of the yield.
Case Report: A Novel Homozygous Mutation in MYF5 Due to Paternal Uniparental Isodisomy of Chromosome 12 in a Case of External Ophthalmoplegia With Rib and Vertebral Anomalies
Authors: Li Qianqian , Zhu Xiaofan , Yu Chenguang , Shang Lin , Li Ranran , Wang Xia , Yang Yaping , Meng Jingjing , Kong Xiangdong
Published: February 2022

External ophthalmoplegia with rib and vertebral anomalies (EORVA) is characterized by congenital nonprogressive external ophthalmoplegia, ptosis, scoliosis, torticollis, vertebral, and rib anomalies, caused by homozygous mutations in the myogenic factor 5 gene (MYF5) located on chromosome 12q21.31. Uniparental disomy (UPD) is a rare inheritance of a pair of chromosomes originating from only one parent. This study describes a case of an 8-year-old boy with ptosis, scoliosis, and dysmorphic hypoplastic ribs with fusion anomalies. Trio-based exome sequencing (trio-ES) identified a novel homozygous mutation c.191delC (p.Ala64Valfs*33) in MYF5 in the proband, with the father being heterozygous and the mother wild-type, as verified by Sanger sequencing. UPD identified from trio-ES variant call format data suggested the possibility of paternal UPD of chromosome 12 (UPD12pat) in the proband, further confirmed to be a complete isodisomy type of UPD by genome-wide single nucleotide polymorphism array. MYF5 was significantly downregulated by 69.14% (**p < 0.01) in HeLa cells transfected with mutant MYF5 containing c.191delC compared to those transfected with the wild-type MYF5, resulting in a truncated protein with a size of ∼20 kDa. In conclusion, this study identified a novel homozygous mutation in MYF5, broadening the genetic spectrum of EORVA and further deepening the understanding of this rare disease.
A Tumor Suppressor Gene-Based Prognostic Classifier Predicts Prognosis, Tumor Immune Infiltration, and Small Molecule Compounds in Breast Cancer
Authors: Jiang Suxiao , Bu Xiangjing , Tang Desheng , Yan Changsheng , Huang Yan , Fang Kun
Published: February 2022

Objective: Tumor suppressor genes (TSGs) play critical roles in the cell cycle checkpoints and in modulating genomic stability. Here, we aimed to develop a TSG-based prognostic classifier for breast cancer.

Methods: Gene expression profiles and clinical information of breast cancer were curated from TCGA (discovery set) and Gene Expression Omnibus (GEO) repository (GSE12093 and GSE17705 datasets as testing sets). Univariate cox regression analysis and random forest machine learning method were presented for screening characteristic TSGs. After multivariate cox regression analyses, a TSG-based prognostic classifier was constructed. The predictive efficacy was verified by C-index and receiver operating characteristic (ROC) curves. Meanwhile, the predictive independency was assessed through uni- and multivariate cox regression analyses and stratified analyses. Tumor immune infiltration was estimated via ESTIMATE and CIBERSORT algorithms. Small molecule agents were predicted through CMap method. Molecular subtypes were clustered based on the top 100 TSGs with the most variance.

Results: A prognostic classifier including nine TSGs was established. High-risk patients were predictive of undesirable prognosis. C-index and ROC curves demonstrated its excellent predictive performance in prognosis. Also, this prognostic classifier was independent of conventional clinicopathological parameters. Low-risk patients exhibited increased infiltration levels of immune cells like T cells CD8. Totally, 48 small molecule compounds were predicted to potentially treat breast cancer. Five TSG-based molecular subtypes were finally constructed, with distinct prognosis and clinicopathological features.

Conclusion: Collectively, this study provided a TSG-based prognostic classifier with the potential to predict clinical outcomes and immune infiltration in breast cancer and identified potential small molecule agents against breast cancer.
Case Report: Cerebral Nocardiosis Caused by Nocardia cyriacigeorgica Detected by Metagenomics in an Apparently Immunocompetent Patient
Authors: Courbin Virginie , Riller Quentin , Amegnizin Jean-Louis , Gricourt Guillaume , Demontant Vanessa , Fihman Vincent , Angebault Cecile , Mahevas Matthieu , Gaube Géraldine , Coutte Laëtitia , Pawlotsky Jean-Michel , Lepeule Raphaël , Rodriguez Christophe , Woerther Paul-Louis
Published: February 2022

We report a case of meningoencephalitis due to Nocardia cyriacigeorgica diagnosed with metagenomics, while all the standard methods were negative. This diagnosis made adaptation of antimicrobial treatment possible and led to the discovery of a rare, acquired immunodeficiency syndrome.
Case Report: IgA Nephropathy in a Patient With Anti-Transcription Intermediary Factor-1γ Antibody-Positive Dermatomyositis
Authors: Zhang Suo , Chen Yu-Lan , Liu Cui-Lian , Xie Jing-Yi , Sun Bao-Dong , Liu Dong-Zhou
Published: February 2022

Immunoglobulin A nephropathy (IgAN) is the most common primary glomerulonephritis characterized by IgA deposits in the mesangial area of glomeruli. Connective tissue disorders are some of the most frequent causes of secondary IgAN. Nevertheless, IgAN rarely occurs in systemic autoimmune myopathies (SAMs). The present case study reports on a 58-year-old patient with dermatomyositis with positive anti-transcription intermediary factor (TIF)-1γ antibodies who was diagnosed with IgAN during standard immunosuppressive therapy. Moreover, we have made a systematic review regarding the association of SAMs and IgAN. To the best of the authors’ knowledge, this is the first case study describing a patient with anti-TIF1γ antibody-positive dermatomyositis who developed IgAN, which demonstrates a potential relationship between anti-TIF1γ-positive dermatomyositis and IgAN. It is important for clinicians to be aware of the possibility of renal involvement in patients with SAMs, even in those with anti-TIF1γ-positive dermatomyositis.
Anti-DFS70 Antibodies Are Associated With Proliferative Lupus Nephritis and Renal Pathological Activity
Authors: Chen Dandan , Zhao Li , Dai Yingxin , Du Fang , Li Enling , Niu Xiangyu , Wang Zhiqing , Zheng Bing , Lu Liangjing
Published: February 2022

Objective

The significance of anti-dense fine speckles 70 (DFS70) antibodies in systemic lupus erythematosus (SLE) is still unclear, especially in lupus nephritis (LN) patients. We investigated the prevalence, clinical and pathological relevance of anti-DFS70 antibodies in LN patients.

Methods

Anti-DFS70 antibodies were measured using enzyme-linked immunosorbent assays in 377 biopsy-proven LN patients, 268 non-LN SLE patients, 232 chronic kidney disease (CKD) patients, and 78 healthy individuals (HI). Demographic, clinical, and pathological parameters were compared between LN patients with and without anti-DFS70 antibodies. Stepwise multivariable logistic regression was performed to identify covariates associated with anti-DFS70 antibodies.

Results

The prevalence of anti-DFS70 antibodies in LN (19.6%) was comparable to non-LN SLE patients (19.8%, P=0.9630), but was significantly higher than CKD patients (13.4%, P=0.0468) and HI (9.0%, P=0.0252). Using multivariable logistic regression analysis, the titer of anti-double-stranded DNA (dsDNA) antibodies (adjusted odds ratio=1.002, 95% confidence interval 1.001-1.003, P=0.004) was associated with positive anti-DFS70 antibodies in LN patients. In addition, anti-DFS70 antibodies were more prevalent in proliferative LN (22.0%, 68/309) compared to membrane LN patients (10.2%, 6/59, P=0.0376). Furthermore, LN patients with positive anti-DFS70 antibodies had significantly higher activity index (AI) compared to patients who were negative (8.0 vs 6.0, P=0.0131). However, the chronicity index was similar between the groups (3.0 vs 3.0, P=0.8412).

Conclusion

Anti-DFS70 antibodies were not associated with LN development in SLE patients but were associated with anti-dsDNA antibodies, proliferative LN, and renal AI. This suggests their potential to serve as a non-histological biomarker for LN subclass and activity status.
Formononetin Protects LPS-Induced Mastitis Through Suppressing Inflammation and Enhancing Blood-Milk Barrier Integrity via AhR-Induced Src Inactivation
Authors: Xiang Kaihe , Shen Peng , Gao Ziyang , Liu Zhuoyu , Hu Xiaoyu , Liu Bin , Fu Yunhe
Published: February 2022

Formononetin (FOR), a natural flavonoid derived from Radix Astragali, has been reported to have anti-inflammatory and anti-oxidative effects. However, its protective mechanism against mastitis is still unknown. Nuclear factor kappa-B (NF-κB) signaling pathway plays an important role in inflammation, especially mastitis. Aryl hydrocarbon receptor (AhR) is involved in inflammatory regulation and defense against diseases. We investigated the protective effect of FOR on LPS-induced mastitis in mice and the effect of Ahr and NF-κB signaling pathways on the development of mastitis. In this study, mastitis model was induced by LPS injection through the nipple duct. Protective effect of FOR on LPS-induced mastitis was assessed by FOR pretreatment. The protective mechanism of FOR against mastitis was further investigated using LPS stimulation on mouse mammary epithelial cells EpH4-Ev. The results showed that LPS-induced mammary histological injury was inhibited by FOR. FOR significantly inhibited LPS-induced MPO activity. FOR administration enhanced the integrity of blood-milk barrier. In vitro and in vivo experiments showed that FOR inhibited LPS-induced NF-κB signaling pathway activation and the production of inflammatory factors TNF-α and IL-1ß. Moreover, FOR increased the expression of tight junction protein and enhanced blood-milk barrier integrity. LPS activated AhR and Src expression. But FOR induced significant increase in AhR inhibited Src phosphorylation to exert anti-inflammatory effects. In addition, AhR antagonist CH223191 reversed the inhibition of FOR on Src expression. And the inhibition of FOR on NF-κB activation and inflammatory cytokine production were reversed by AhR antagonist CH223191. In conclusion, FOR had protective effects against LPS-induced mastitis via suppressing inflammation and enhancing blood-milk barrier integrity via AhR-induced Src inactivation.
Cross Talk Between Natural Killer T and Dendritic Cells and Its Impact on T Cell Responses in Infections
Authors: Zhao Lei , Yang Xi
Published: February 2022

Both innate and adaptive immunity is vital for host defense against infections. Dendritic cells (DCs) are critical for initiating and modulating adaptive immunity, especially for T-cell responses. Natural killer T (NKT) cells are a small population of innate-like T cells distributed in multiple organs. Many studies have suggested that the cross-talk between these two immune cells is critical for immunobiology and host defense mechanisms. Not only can DCs influence the activation/function of NKT cells, but NKT cells can feedback on DCs also, thus modulating the phenotype and function of DCs and DC subsets. This functional feedback of NKT cells on DCs, especially the preferential promoting effect on CD8α+ and CD103+ DC subsets in lymphoid and non-lymphoid tissues, significantly impacts the systemic and local adaptive CD4 and CD8 T cell responses in infections. This review focuses on the two-way interaction between NKT cells and DCs, emphasizing the importance of NKT cell feedback on DCs in bridging innate and adaptive immune responses for host defense purposes.
Effect of High Strain Rates on Adiabatic Shear Bands Evolution and Mechanical Performance of Dual-Phase Ti Alloy
Authors: Hao Fang , Du Yuxuan , Wang William Yi , Mao Youchuan , Yin Junlei , Zou Chengxiong , Chen Haisheng , Wang Kaixuan , Feng Yong , Liu Xianghong , Li Jinshan
Published: February 2022

In the present work, the adiabatic shear characteristics of our recently designed α + β dual-phase Ti alloy at different strain rates have been investigated by hat shaped specimen. The deformation process is divided into three stages: work hardening stage, steady stage, and unstable thermal softening stage. Along or near the shear deformation paths, the microvoids and the cracks can be captured at the strain rate of 1.8 × 104 s−1, 2.0 × 104 s−1, and 2.3 × 104 s−1, both of which contribute to the stable and unstable softening. It is found that dynamic stored energy of cold work will be significantly improved by the enhanced high strain rate. In the view of coupling analysis of inverse pole figure and grain boundary map, it seems that low angle grain boundaries present a good resistance to the formation of cracks and thermal softening. On the contrary, high angles grain boundaries are typically located in ASBs and their affecting regions, which is in line with the reported results. While the geometrical necessary dislocation (GND) density of adiabatic shear band (ASB) and its surroundings increased significantly, the width of the ASB becomes wider as the strain rate increases, which is consistent with the theory of sub-grain rotation dynamic recrystallization model. The formation of multiple ASBs in the corner position is schematically illustrated and the average elastic modulus and hardness of the ASB region are lower than the α and β phases, combined with the GND analysis, which proves that the ASB is a thermal softening zone in this experiment.
The Innovative Self-Sensing Strain Sensor for Asphalt Pavement Structure: Substitutability and Synergy Effects of Graphene Platelets With Carbon Nanotubes in Epoxy Composites
Authors: Xin Xue , Luan Xuehao , Su Linping , Ma Chuanyi , Liang Ming , Ding Ximao , Yao Zhanyong
Published: February 2022

Situ sensors with high accuracy, long durability, and high survival rate are crucial for the health monitoring of asphalt pavement. Due to the harsh environment during the construction period and service life, the monitoring components which can be buried synchronously with the construction period of the road surface become a difficult problem to be solved urgently. The development of functional composites sheds a new insight for pavement strain detection with remarkable self-sensing behavior. In this paper, the substitutability and synergy effect of graphene platelets with carbon nanotubes (CNTs), the effect of CNT types with different specific surface areas in epoxy composites to the morphological, electrical, and mechanical properties, and the strain-electrical resistance response peculiarity of composites were evaluated. The performance of developed composite sensors with epoxy encapsulation was investigated through laboratory experiments. The morphologies showed that CNT-GNP hybrids in composites present a better dispersion state because of the size effect and synergetic effect whereas the pure CNTs are prone to entangle with each other. Composites with CNT(SSA500) display the most amounts of conductive units in same dosage. CNTs and GNP can strengthen the elastic modulus of the epoxy matrix to basically the same as that of asphalt mixture within the range of 1100–1500 MPa. At last, Laboratory experiments have proved the promising prospect for CNTs-GNP/epoxy composites serving as the strain sensor. The developed composites-based strain sensor can provide a new prospect for asphalt pavement monitoring.
Comparison of Various Vagal Maneuvers for Supraventricular Tachycardia by Network Meta-Analysis
Authors: Huang Edward Pei-Chuan , Chen Chi-Hsin , Fan Cheng-Yi , Sung Chih-Wei , Lai Pei Chun , Huang Yen Ta
Published: February 2022

Background

Vagal maneuvers (VagMs) are recommended as the first-line treatment of supraventricular tachycardia (SVT). However, the optimal type of VagMs remains unproven.

Aim

This study aims to compare the effectiveness and adverse events amongst VagMs on SVT via network meta-analyses (NMAs).

Methods

We systematically searched randomized controlled trials (RCTs) that involved adults with SVT and compared VagMs without language restrictions. We determined the initial and final responses of conversion rate to sinus rhythm and adverse events. Risk of bias (RoB) was appraised by Cochrane revised tool, and contribution matrix was calculated. NMAs were synthesized using frequentist random-effects model and presented as relative risk (RR) with 95% CI. The order of probability was presented as surface under the cumulative ranking curve analysis (SUCRA). Sensitivity analysis was performed using both Bayesian and frequentist approach with fixed- or random-effects models. Certainty of evidence (CoE) was rated by using the Grading of Recommendations, Assessment, Development, and Evaluations methodology.

Results

Fourteen RCTs with 2,180 patients were enrolled. Small portion of mixed estimates was contributed from high overall RoB studies. Compared with carotid sinus massage (CSM), the modified Valsalva maneuver (MVM) was the most effective VagM after initial performance [SUCRA: 0.9992, RR: 5.47 (1.77–16.93)] and at the end of study [SUCRA: 1.0000, RR: 3.62 (2.04–6.39), CoE: high]. The standard VM did not elicit better conversion rate to the sinus rhythm than CSM at the initial response [SUCRA: 0.4395, RR: 1.97 (0.63–6.15)] and at the end of the study [SUCRA: 0.4795, RR: 1.64 (0.94–2.87), CoE: moderate]. The SUCRA value of CSM at the initial and final responses was the least one amongst three VagMs (0.0613 and 0.0205, respectively). Adverse events amongst three VagMs were similar (CoE: low). Sensitivity analyses yielded consistent results.

Conclusion

We recommended MVM as the first choice of VagM for rhythm conversion before the pharmacological management of SVT.
Emergence of Meropenem Resistance Among Cefotaxime Non-susceptible Streptococcus pneumoniae: Evidence and Challenges
Authors: Varghese Rosemol , Basu Soumya , Neeravi Ayyanraj , Pragasam Agilakumari , Aravind V. , Gupta Richa , Miraclin Angel , Ramaiah Sudha , Anbarasu Anand , Veeraraghavan Balaji
Published: February 2022

The principal causative agent of acute bacterial meningitis (ABM) in children and the elderly is Streptococcus pneumoniae, with a widespread increase in penicillin resistance. Resistance is due to non-synonymous single-nucleotide polymorphisms (nsSNPs) that alter the penicillin-binding proteins (PBPs), the targets for all β-lactam drugs. Hence, resistance against one β-lactam antibiotic may positively select another. Since meropenem is an alternative to cefotaxime in meningeal infections, we aim to identify whether nsSNPs in the PBPs causing penicillin and cefotaxime resistance can decrease the pneumococcal susceptibility to meropenem. Comparison of the nsSNPs in the PBPs between the cefotaxime-resistant Indian (n = 33) and global isolates (n = 28) revealed that nsSNPs in PBP1A alone elevated meropenem minimal inhibitory concentrations (MICs) to 0.12 μg/ml, and nsSNPs in both PBP2X and 2B combined with PBP1A increases MIC to ≥ 0.25 μg/ml. Molecular docking confirmed the decrease in the PBP drug binding affinity due to the nsSNPs, thereby increasing the inhibition potential and the MIC values, leading to resistance. Structural dynamics and thermodynamic stability pattern in PBPs as a result of mutations further depicted that the accumulation of certain nsSNPs in the functional domains reduced the drug affinity without majorly affecting the overall stability of the proteins. Restricting meropenem usage and promoting combination therapy with antibiotics having non-PBPs as targets to treat cefotaxime non-susceptible S. pneumoniae meningitis can prevent the selection of β-lactam resistance.
Membrane Vesicles Derived From Clostridium botulinum and Related Clostridial Species Induce Innate Immune Responses via MyD88/TRIF Signaling in vitro
Authors: Kobayashi Nobuhide , Abe Kimihiro , Akagi Sachiyo , Kitamura Mayu , Shiraishi Yoshitake , Yamaguchi Aki , Yutani Masahiro , Amatsu Sho , Matsumura Takuhiro , Nomura Nobuhiko , Ozaki Noriyuki , Obana Nozomu , Fujinaga Yukako
Published: February 2022

Clostridium botulinum produces botulinum neurotoxin complexes that cause botulism. Previous studies elucidated the molecular pathogenesis of botulinum neurotoxin complexes; however, it currently remains unclear whether other components of the bacterium affect host cells. Recent studies provided insights into the role of bacterial membrane vesicles (MVs) produced by some bacterial species in host immunity and pathology. We herein examined and compared the cellular effects of MVs isolated from four strains of C. botulinum with those of closely related Clostridium sporogenes and two strains of the symbiont Clostridium scindens. MVs derived from all strains induced inflammatory cytokine expression in intestinal epithelial and macrophage cell lines. Cytokine expression was dependent on myeloid differentiation primary response (MyD) 88 and TIR-domain-containing adapter-inducing interferon-β (TRIF), essential adaptors for toll-like receptors (TLRs), and TLR1/2/4. The inhibition of actin polymerization impeded the uptake of MVs in RAW264.7 cells, however, did not reduce the induction of cytokine expression. On the other hand, the inhibition of dynamin or phosphatidylinositol-3 kinase (PI3K) suppressed the induction of cytokine expression by MVs, suggesting the importance of these factors downstream of TLR signaling. MVs also induced expression of Reg3 family antimicrobial peptides via MyD88/TRIF signaling in primary cultured mouse small intestinal epithelial cells (IECs). The present results indicate that MVs from C. botulinum and related clostridial species induce host innate immune responses.
Altered Interplay Among Large-Scale Brain Functional Networks Modulates Multi-Domain Anosognosia in Early Alzheimer’s Disease
Authors: Valera-Bermejo Jose Manuel , De Marco Matteo , Venneri Annalena
Published: February 2022

Decline in self-awareness is a prevalent symptom in Alzheimer’s disease (AD). Current data suggest that an early breakdown in the brain’s default mode network (DMN) is closely associated with the main symptomatic features in AD patients. In parallel, the integrity of the DMN has been shown to be heavily implicated in retained self-awareness abilities in healthy individuals and AD patients. However, the global contribution to awareness skills of other large-scale networks is still poorly understood. Resting-state functional magnetic resonance imaging (rs-fMRI) scans were acquired and pre-processed from 53 early-stage AD individuals. A group-level independent component analysis was run to isolate and reconstruct four intrinsic connectivity large-scale brain functional networks, namely left and right central executive fronto-parietal networks (FPN), salience network, and anterior and posterior DMN. Hypothesis-driven seed-based connectivity analyses were run to clarify the region-specific underpinnings of multi-domain anosognosia. Multiple regression models were run on large-scale network- and seed-based connectivity maps, including scores of memory, non-memory and total anosognosia obtained via the Measurement of Anosognosia Questionnaire. Memory anosognosia scores were associated with selective lower fronto-temporal connectivity and higher parieto-temporal connectivity. Non-memory anosognosia scores were associated with higher connectivity between the anterior DMN and the cerebellum, between the left medial prefrontal seeds and the contralateral prefrontal cortex, and between the left hippocampal seed and the left insula; lower connectivity was observed between the right prefrontal cortex and the right lingual seed. Lastly, total anosognosia scores were associated with large-scale network alterations, namely reduced left-FPN expression in the left posterior cingulate, reduced right-FPN expression in the left inferior lingual gyrus and adjacent inferior occipital cortex, and increased right-FPN expression in the right anterior cingulate. Seed-based analyses yielded significant connectivity differences only in the connectivity pattern associated with the left hippocampal seed by displaying lower intercommunication with the right prefrontal cortex, but higher connectivity with the left caudate nucleus. These findings support the hypothesis that alterations in functional connectivity of frontal lobe regions involved in executive-related mechanisms represent the neural correlates of domain-specific anosognosia in early AD. Up-regulated connectivity with subcortical structures appears to contribute to changes in the network dynamics interplay and fosters the appearance of anosognosia.
Digital Dance for People With Parkinson's Disease During the COVID-19 Pandemic: A Feasibility Study
Authors: Walton Lois , Domellöf Magdalena Eriksson , Åström Åsa N. , Elowson Åsa , Neely Anna Stigsdotter
Published: February 2022

Background

Dance as a treatment to support physical, cognitive and emotional functioning, has gained increased acceptance as a healthcare intervention for people with Parkinson's Disease (PD). The impact of the COVID-19 pandemic has been far reaching with devastating effects for at-risk populations. To find alternative and safe treatment delivery options during the pandemic has been of utmost importance. The purpose of this mixed-methods study was to evaluate the feasibility and the experience of digital dance for people with PD (Dance for PD©) and to examine change in self-reported quality of life, psychological health, subjective cognitive complaints and mental fatigue.

Methods

23 participants with PD (mean age 70) partook in 10-h weekly digital Dance for PD sessions. Feasibility outcome measures were assessed at post-test. Web-based questionnaires examining quality of life, subjective memory complaints, depression, anxiety and mental fatigue were administered at pre- and post-test. Moreover, nine participants partook in focus group discussions at post-test.

Results

The results showed an acceptable feasibility to home-based digital Dance for PD, where 86% of the dance classes were completed, only minor negative side effects were reported (i.e., sore joints), and all experienced the dance classes as motivating and safe to do at home. The majority also reported positive effects on mood and physical functioning. The results from the questionnaires showed significant improvements in depressive symptoms (p = 0.006) and quality of life (p < 0.001) at post-test. In the focus groups, participants indicated that digital dance was a beneficial and enjoyable activity with a strong added value during the COVID-19 pandemic. Nevertheless, they experienced that digital dance missed some important elements of live dance.

Conclusions

This study showed that digital Dance for PD is feasible and holds promise as a viable and safe method to keep people with PD dancing even when physical meetings are not possible. Beyond the pandemic, digital dance could be applied to a wide variety of patient groups including rural populations and patients for whom transportation may not be feasible for practical or financial reasons.

Trial Registration

Retrospectively registered at ClinicalTrials.gov on 25/06/2021 with the following registration number: NCT04942392.
Characterization of Antigen-Induced CD4+ T-Cell Senescence in Multiple Sclerosis
Authors: Tomas-Ojer Paula , Puthenparampil Marco , Cruciani Carolina , Docampo María José , Martin Roland , Sospedra Mireia
Published: February 2022

Antigen-induced T-cell exhaustion and T-cell senescence are peripheral regulatory mechanisms that control effector T-cell responses. Markers of exhaustion and senescence on T Cells indicate the previous activation by repetitive stimulation with specific antigens. Malignant tumors are accompanied by enhanced T-cell exhaustion and T-cell senescence resulting in immune evasion, while these control mechanisms might be diminished in autoimmune diseases including multiple sclerosis (MS). To better understand the involvement of antigen-induced T-cell senescence in controlling CD4+ T-cell-mediated autoimmune responses in MS, we have analyzed the re-expression of CD45RA and the downregulation of CD28 and CD27 molecules as markers of antigen-induced T-cell senescence in fresh cerebrospinal fluid (CSF)-infiltrating and paired circulating T cells from patients with MS. Patients with different levels of CD4+ T-cell senescence were identified and characterized regarding demographical and clinical features as well as intrathecal markers of neurodegeneration. CD4+ T-cell senescence was also analyzed in control patients to explore a putative deficit of this regulatory mechanism in MS. This study shows heterogeneity of markers of CD4+ T-cell senescence in patients with MS. Patients with high levels of CD4+ T-cell senescence in peripheral blood showed increased frequencies of CSF-infiltrating CD28+ CD27-EM CD4+ T cells with a proinflammatory Th1 functional phenotype. The correlation of these cells with the intrathecal levels of neurofilament light chain, a marker of neurodegeneration, suggests their relevance in disease pathogenesis and the involvement of T-cell senescence in their regulation. Markers of antigen-induced T-senescence, therefore, show promise as a tool to identify pathogenic CD4+ T cells in patients with MS.