bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Approach to Enhance the Generalization Capability of the Hourly Solar Diffuse Horizontal Irradiance Models on Diverse Climates
Authors: Kalyanam Raghuram , Hoffmann Sabine
Published: September 2020

Solar radiation data is essential for the development of many solar energy applications ranging from thermal collectors to building simulation tools, but its availability is limited, especially the diffuse radiation component. There are several studies aimed at predicting this value, but very few studies cover the generalizability of such models on varying climates. Our study investigates how well these models generalize and also show how to enhance their generalizability on different climates. Since machine learning approaches are known to generalize well, we apply them to truly understand how well they perform on different climates than they are originally trained. Therefore, we trained them on datasets from the U.S. and tested on several European climates. The machine learning model that is developed for U.S. climates not only showed low mean absolute error (MAE) of 23 W/m2, but also generalized very well on European climates with MAE in the range of 20 to 27 W/m2. Further investigation into the factors influencing the generalizability revealed that careful selection of the training data can improve the results significantly.
Tesla Bladed Pump (Disc Bladed Pump) Preliminary Experimental Performance Analysis
Authors: Heng Yaguang , Han Yuming , Zhang Huiyu , Zhang Weibin , Bois Gérard , Jiang Qifeng , Wang Zhengwei , Liu Xiaobing
Published: September 2020

A preliminary experimental work on a centrifugal pump model specifically designed to transport slurry and multiphase flows is presented. The impeller design corresponds to the so-called “Tesla Bladed Pump” adapted from an existing DiscflowTM pump design for the petroleum deep-sea application. The overall performance results of such a specific pump design are presented for different rotational speeds and discussed in relation to affinity laws coefficients. The results show that the performance of the tested disc pump strongly differs from the conventional centrifugal bladed pump. A one-dimensional approach using complete Euler relation is used to explain the differences in the present case. Moreover, it has been found that available results in open literatures do not correspond to the real optimum conditions, more detailed research work must be performed to get a better understanding on this kind of bladed disc pump.
Sequential Tasks Shifting for Participation in Demand Response Programs
Authors: Khorram Mahsa , Faria Pedro , Vale Zita , Ramos Carlos
Published: September 2020

In this paper, the proposed methodology minimizes the electricity cost of a laundry room by means of load shifting. The laundry room is equipped with washing machines, dryers, and irons. Additionally, the optimization model handles demand response signals, respecting user preferences while providing the required demand reduction. The sequence of devices operation is also modeled, ensuring correct operation cycles of different types of devices which are not allowed to overlap or have sequence rules. The implemented demand response program specifies a power consumption limit in each period and offers discounts for energy prices as incentives. In addition, users can define the required number of operations for each device in specific periods, and the preferences regarding the operation of consecutive days. In the case study, results have been obtained regarding six scenarios that have been defined to survey about effects of different energy tariffs, power limitations, and incentives, in a laundry room equipped with three washing machines, two dryers, and one iron. A sensitivity analysis of the power consumption limit is presented. The results show that the proposed methodology is able to accommodate the implemented scenario, respecting user preferences and demand response program, minimizing energy costs. The final electricity price has been calculated for all scenarios to discuss the more effective schedule in each scenario.
Anaerobic Co-Digestion Effluent as Substrate for Chlorella vulgaris and Scenedesmus obliquus Cultivation
Authors: Scarponi Paolina , Bonetto Alessandro , Bolzonella David , Astals Sergi , Cavinato Cristina
Published: September 2020

Anaerobic digestate supernatant can be used as a nutrient source for microalgae cultivation, thus integrating phytoremediation processes with high value products storage in microalgae biomass. Microalgae are able to use nitrogen and phosphorous from digestate, but high nutrient concentration can cause growth inhibition. In this study, two microalgae strains (C. vulgaris and S. obliquus) were cultivated on the anaerobic co-digestion supernatant (obtained from the organic fraction of municipal solid waste (OFMSW) and waste activated sludge (WAS)) in a preliminary Petri plate screening at different dilutions (1:10 and 1:5) using a synthetic medium (ISO) and tap water (TW). Direct Nile red screening was applied on colonies to preliminarily identify hydrophobic compound storage and then a batch test was performed (without air insufflation). Results show that C. vulgaris was able to grow on digestate supernatant 1:5 diluted, while Nile red screening allowed the preliminary detection of hydrophobic compound storage in colonies. The analysis carried out at the end of the test on ammonia, phosphate, nitrate and sulphate showed a removal percentage of 47.5 ± 0.8%, 65.0 ± 6.0%, 95.0 ± 3.0% and 99.5 ± 0.1%, respectively.
Differentiation of the Level of Sustainable Development of Energy Markets in the European Union Countries
Authors: Bluszcz Anna , Manowska Anna
Published: September 2020

The economic development of the EU’s countries depends on stable and permanent access to various energy sources. The integration of European energy markets is a long-term process. Each member country has a different energy balance, energy resources and electricity generation technologies. Therefore, comparing Member States with one another is a complex task. The article deals with organizing the set of objects in multidimensional spaces of variables from the point of view of a certain characteristics that cannot be measured in a simple way, i.e., the relative level of the development of energy markets. The following diagnostic variables were adopted for the analysis: consumption of electric energy generated from renewables per capita (TWH/person), hard coal consumption (million tons/person), greenhouse gas emissions per capita, available for final consumption gigawatt-hour per person, final energy consumption thousand tons of oil equivalent (TOE) per person, petroleum available for final consumption (gigawatt-hour), natural gas (terajoule gross calorific value-GCV) per person, energy intensity of GDP (kilograms of oil equivalent (KGOE) per thousand euro, import dependency %. The article draws upon the cluster analysis, which uses the methods and techniques that enable to extract such objects (countries) from the data set, which would be similar to each other and combine them into groups. The purpose of the article is to examine and discus the diversity of Member States on the level of development of energy markets taking into account the specific situation of Poland. The presented model has the potential to be used at the level of enterprises from various industries to evaluate the level of sustainable development. The algorithm modification will only require the selection of available diagnostic variables describing the given enterprise.
Measuring the Risk of Supply and Demand Imbalance at the Monthly to Seasonal Scale in France
Authors: Alonzo Bastien , Drobinski Philippe , Plougonven Riwal , Tankov Peter
Published: September 2020

Transmission system operator (TSOs) need to project the system state at the seasonal scale to evaluate the risk of supply-demand imbalance for the season to come. Seasonal planning of the electricity system is currently mainly adressed using climatological approach to handle variability of consumption and production. Our study addresses the need for quantitative measures of the risk of supply-demand imbalance, exploring the use of sub-seasonal to seasonal forecasts which have hitherto not been exploited for this purpose. In this study, the risk of supply-demand imbalance is defined using exclusively the wind energy production and the consumption peak at 7 pm. To forecast the risks of supply-demand imbalance at monthly to seasonal time horizons, a statistical model is developed to reconstruct the joint probability of consumption and production. It is based on a the conditional probability of production and consumption with respect to indexes obtained from a linear regression of principal components of large-scale atmospheric predictors. By integrating the joint probability of consumption and production over different areas, we define two kind of risk measures: one quantifies the probablity of deviating from the climatological means, while the other, which is the value at risk at 95% confidence level (VaR95) of the difference between consumption and production, quantifies extreme risks of imbalance. In the first case, the reconstructed risk accurately reproduces the actual risk with over 0.80 correlation in time, and a hit rate around 70–80%. In the second case, we find a mean absolute error (MAE) between the reconstructed and real extreme risk of 2.5 to 2.8 GW, a coefficient of variation of the root mean square error (CV-RMSE) of 3.8% to 4.2% of the mean actual VaR95 and a correlation of 0.69 and 0.66 for winter and fall, respectively. By applying our model to ensemble forecasts performed with a numerical weather prediction model, we show that forecasted risk measures up to 1 month horizon can outperform the climatology often used as the reference forecast (time correlation with actual risk ranging between 0.54 and 0.82). At seasonal time horizon (3 months), our forecasts seem to tend to the climatology.
Climate Policy Paralysis in Australia: Energy Security, Energy Poverty and Jobs
Authors: Ali Saleem H. , Svobodova Kamila , Everingham Jo-Anne , Altingoz Mehmet
Published: September 2020

According to the 2020 Climate Change Performance Index, Australia was ranked as the worst-performing country on climate change policy. The country has an ambivalent record of climate policy development as well as implementation, and has been criticized for its inaction. This paper considers why the country has been locked in climate policy “paralysis” through analyzing defining attributes of such a paralysis, and the tentative connections between domestic energy policies and international trade and development. We conducted a media content analysis of 222 articles and identified media narratives in three cases of energy projects in the country involving thermal coal exports, domestic renewable energy storage, and closure of a domestic coal power station. The analysis reveals that policy paralysis in Australian climate change policy can be traced back to the countervailing arguments that have been pervasive around domestic energy security, rural employment and international energy poverty. The political establishment has struggled to develop a sustainable consensus on climate change and the citizenry remains polarized. We also discuss how a “focusing event,” such as a major natural disaster can break the impasse but this is only possible if energy security at home, energy poverty abroad and employment imperatives across the board are clearly delineated, measured and prioritized.
Ultra-Short-Term Load Demand Forecast Model Framework Based on Deep Learning
Authors: Li Hongze , Liu Hongyu , Ji Hongyan , Zhang Shiying , Li Pengfei
Published: September 2020

Ultra-short-term load demand forecasting is significant to the rapid response and real-time dispatching of the power demand side. Considering too many random factors that affect the load, this paper combines convolution, long short-term memory (LSTM), and gated recurrent unit (GRU) algorithms to propose an ultra-short-term load forecasting model based on deep learning. Firstly, more than 100,000 pieces of historical load and meteorological data from Beijing in the three years from 2016 to 2018 were collected, and the meteorological data were divided into 18 types considering the actual meteorological characteristics of Beijing. Secondly, after the standardized processing of the time-series samples, the convolution filter was used to extract the features of the high-order samples to reduce the number of training parameters. On this basis, the LSTM layer and GRU layer were used for modeling based on time series. A dropout layer was introduced after each layer to reduce the risk of overfitting. Finally, load prediction results were output as a dense layer. In the model training process, the mean square error (MSE) was used as the objective optimization function to train the deep learning model and find the optimal super parameter. In addition, based on the average training time, training error, and prediction error, this paper verifies the effectiveness and practicability of the load prediction model proposed under the deep learning structure in this paper by comparing it with four other models including GRU, LSTM, Conv-GRU, and Conv-LSTM.
An Analysis of the Current Status of Woody Biomass Gasification Power Generation in Japan
Authors: Baba Yasutsugu , Pandyaswargo Andante Hadi , Onoda Hiroshi
Published: September 2020

Forests cover two-thirds of Japan’s land area, and woody biomass is attracting attention as one of the most promising renewable energy sources in the country. The Feed-in Tariff (FIT) Act came into effect in 2012, and since then, woody biomass power generation has spread rapidly. Gasification power generation, which can generate electricity on a relatively small scale, has attracted a lot of attention. However, the technical issues of this technology remain poorly defined. This paper aims to clarify the problems of woody biomass gasification power generation in Japan, specifically on the challenges of improving energy utilization rate, the problem of controlling the moisture content, and the different performance of power generation facilities that uses different tree species. We also describe the technological development of a 2 MW updraft reactor for gasification and bio-oil coproduction to improve the energy utilization rate. The lower heating value of bio-oil, which was obtained in the experiment, was found to be about 70% of A-fuel oil. Among the results, the importance of controlling the moisture content of wood chips is identified from the measurement evaluation of a 0.36 MW-scale downdraft gasifier’s actual operation. We discuss the effects of tree species variation and ash on gasification power generation based on the results of pyrolysis analysis, industry analysis for each tree species. These results indicate the necessity of building a system specifically suited to Japan’s climate and forestry industry to allow woody biomass gasification power generation to become widespread in Japan.
Chicken Egg White—Advancing from Food to Skin Health Therapy: Optimization of Hydrolysis Condition and Identification of Tyrosinase Inhibitor Peptides
Authors: Yap Pei-Gee , Gan Chee-Yuen
Published: September 2020

Active fragments (bioactive peptides) from the chicken egg white proteins were expected to exert tyrosinase inhibitory activities in which skin hyperpigmentation could be prevented. Egg white was hydrolyzed by trypsin, chymotrypsin and the combination of both enzymes. The enzyme treatments achieved >50% degree of hydrolysis (DH) at substrate-to-enzyme (S/E) ratio of 10–30 (w/w) and hydrolysis time of 2–5 h. A crossed D-optimal experimental design was then used to determine the optimal enzyme composition, S/E ratio and hydrolysis time in order to yield hydrolysates with strong monophenolase and diphenolase inhibitory activities. The optimized conditions 55% trypsin, 45% chymotrypsin, S/E 10:1 w/w and 2 h achieved 45.9% monophenolase activity inhibition whereas 100% trypsin, S/E 22.13:1 w/w and 3.18 h achieved 48.1% diphenolase activity inhibition. LC/MS and MS/MS analyses identified the peptide sequences and the subsequent screening had identified 7 peptides (ILELPFASGDLLML, GYSLGNWVCAAK, YFGYTGALRCLV, HIATNAVLFFGR, FMMFESQNKDLLFK, SGALHCLK and YFGYTGALR) as the potential inhibitor peptides. These peptides were able to bind to H85, H94, H259, H263, and H296 (hotspots for active residues) as well as F92, M280 and F292 (stabilizing residues) of tyrosinase based on structure-activity relationship analysis. These findings demonstrated the potential of egg white-derived bioactive peptides as skin health therapy.
The Link between the Consumer and the Innovations in Food Product Development
Authors: Guiné Raquel P. F. , Florença Sofia G. , Barroca Maria João , Anjos Ofélia
Published: September 2020

New lifestyles, higher incomes and better consumer awareness are increasing the demand for a year-round supply of innovative food products. In past decades, important developments have been achieved in areas related to food and the food industry. This review shows that factors influencing performance in new product development (NPD) are dynamic and continuously guiding project development. The data obtained by direct involvement of consumers can impact positively successful product development and enhance the company’s financial performance. The study of consumer behaviour and attitudes towards new foods encompasses multiple aspects, such as preference, choice, desire to eat certain foods, buying intentions and frequency of consumption. Additionally, both the consumers’ willingness to purchase and the willingness to pay a premium are important in NPD, launching and success.
An Economic Evaluation Framework for Government-Funded Home Adaptation Schemes: A Quantitative Approach
Authors: Zhang Bo , Zhou Peng
Published: September 2020

The ability to live independently plays a crucial role in the mental and psychological wellbeing of the disabled. To achieve this goal, most governments spend a substantial budget on home adaptation projects. It has been observed that schemes with different target clients (residents versus landlords) are different in efficiency and effectiveness. To understand why and how these schemes differ in performance, this paper develops and applies a generic economic evaluation framework for government-funded home adaptation schemes. Based on the individual-level surveys collected in the United Kingdom, an empirical model was formulated to quantify the determinants for various performance indicators, including money costs, time costs and client satisfaction. Robust estimation procedures were applied to deal with the heteroscedasticity and outlier problems in the data. Results showed that a specialized independent living scheme dedicated to disability adaptations (e.g., the Physical Adaptations Grant, PAG) had higher efficiency and effectiveness than general-purpose schemes (e.g., the Disabled Facilities Grant, DFG), because the funds were provided to the landlords who had a stronger motivation to minimize the time cost in the short run and maximize the future rent potential in the long run. A “unified system” approach to adaptations should be a guiding principle for policy development, regardless of who actually delivers the service.
The Effect of Discrimination on Stress among Public Housing Residents: A Comparative Study between Social-Mix and Independent Public Housing Complexes
Authors: Jun Hee-Jung , Han Soojeong
Published: September 2020

This study aims to examine the differential effect of discrimination on stress between social-mix and independent public housing complexes. We analyzed the 2017 Seoul Public Housing Residents Panel Study data that were collected from public housing residents living in Seoul, Korea by running ordinal logit analyses. The empirical analysis shows that discrimination has a lower effect on stress in social-mix housing complexes than in independent public housing complexes. In addition, the moderating effect of community-based activities on the relationship between discrimination and stress was found in the independent public housing complex model.
Childcare Food Provision Recommendations Vary across Australia: Jurisdictional Comparison and Nutrition Expert Perspectives
Authors: Spence Alison , Love Penelope , Byrne Rebecca , Wakem Amy , Matwiejczyk Louisa , Devine Amanda , Golley Rebecca , Sambell Ros
Published: September 2020

Early childhood is a critical stage for nutrition promotion, and childcare settings have the potential for wide-reaching impact on food intake. There are currently no Australian national guidelines for childcare food provision, and the comparability of existing guidelines across jurisdictions is unknown. This project aimed to map and compare childcare food provision guidelines and to explore perspectives amongst early childhood nutrition experts for alignment of jurisdictional childcare food provision guidelines with the Australian Dietary Guidelines (ADG). A desktop review was conducted and formed the basis of an online survey. A national convenience sample of childhood nutrition experts was surveyed. Existing guideline recommendations for food group serving quantities were similar across jurisdictions but contained many minor differences. Of the 49 survey respondents, most (84–100%) agreed with aligning food group provision recommendations to provide at least 50% of the recommended ADG serves for children. Most (94%) agreed that discretionary foods should be offered less than once per month or never. Jurisdictional childcare food provision guidelines do not currently align, raising challenges for national accreditation and the provision of support and resources for services across jurisdictions. Childhood nutrition experts support national alignment of food provision guidelines with the ADG.
Hyperhomocysteinemia Concurrent with Metabolic Syndrome Is Independently Associated with Chronic Kidney Disease among Community-Dwelling Adults in an Urban Korean Population
Authors: Moon Hana , Ko Hae-Jin , Kim A-Sol
Published: September 2020

Elevated homocysteine (Hcy) levels and metabolic syndrome (MetS) are associated with chronic kidney disease (CKD). We investigated the combined effects of hyperhomocysteinemia (HHcy) and MetS on CKD among community-dwelling adults in an urban area of South Korea. We also identified the combination of HHcy and individual MetS components associated with the maximal risk of CKD. A retrospective cross-sectional study involving 19,311 health examinees between 2 January 2011 and 31 December 2015 was conducted. The participants were divided into four groups—namely, the HHcy−/MetS−, HHcy−/MetS+, HHcy+/MetS−, and HHcy+/MetS+ groups. CKD was defined as a low eGFR <60 mL/min/1.73 m2 or albuminuria. The HHcy+/MetS+ group had a higher risk of CKD than the HHcy−/MetS+ group (odds ratio (OR): 1.750, p = 0.002 for males; OR: 3.224, p < 0.001 for females). The HHcy+/MetS+ group had a higher CKD risk than the HHcy+/MetS− group; however, the difference was not statistically significant (OR: 1.070, p = 0.712 for males; OR: 1.847, and p < 0.074 for females). HHcy concurrent with MetS increased the CKD risk. Among the combinations of HHcy and MetS components, the coexistence of HHcy and central obesity had the greatest effect on CKD. Therefore, the timely detection and treatment of HHcy and MetS are important for preventing CKD.
The Relationship between Muscular Strength and Depression in Older Adults with Chronic Disease Comorbidity
Authors: Lee Jae-Moo , J. Ryan Edward
Published: September 2020

Older adults with disease and disability are particularly vulnerable to experiencing more severe consequences of depression. The purpose of the present study is to investigate the relationship between disease comorbidities (number of disease: ND0, 1 disease: ND1 and 2 or more diseases: ND ≥ 2), hand grip strength (low HGS and high HGS), and the prevalence of depression in Korean older adults. Data from the living profiles of older people survey that was conducted by the ministry of health and welfare in Korea were utilized. Data for 6107 females and 4347 males were appropriate for statistical tests. The results demonstrated that depression was more prevalent as ND increased (p < 0.01). In addition, HGS appeared lower as ND increased in both male (p < 0.01) and female subjects (p < 0.01). Furthermore, relative to ND0 and low HGS, ND0 and high HGS showed a ~65% reduction in the prevalence of depression (p < 0.01). After adjusting for age, the prevalence of depression was reduced by ~60% in the subgroup with ND0 and high HGS relative to ND0 and low HGS (p < 0.01). These data demonstrate that muscular strength indices such as HGS may be useful when assessing depression in older adults. Further research in this area is warranted.
Multi-Objective Optimization of a Regional Water–Energy–Food System Considering Environmental Constraints: A Case Study of Inner Mongolia, China
Authors: Chen Junfei , Ding Tonghui , Li Ming , Wang Huimin
Published: September 2020

Water, energy, and food, as the basic material resources of human production and life, play a prominent role in social and economic development. As the imbalance between the supply and demand of water, energy, and food increases, a highly sensitive and fragile relationship gradually forms among water, energy, and food. In this paper, Inner Mongolia in China is selected as a research area. Firstly, synergy theory is applied to establish the framework of a water–energy–food system. Then, a multi-objective programming model is constructed, where the objective functions are defined to minimize the integrated deviation degree and pollutant emissions of the water–energy–food system. Meanwhile, maximization of the water benefit, energy production, and food production is also considered. In addition, the model takes economy, environment, water, energy, and food as constraints. Finally, a genetic algorithm is designed for accurately assessing the most promising results. The results show that the cooperation degree of the water–energy–food system in Inner Mongolia is getting better and better, and the pollutant emission from the water–energy–food system is decreasing. In 2020, the proportion of agricultural water consumption fell by 1%, while that of industrial water consumption rose by 0.48%. The production of coal, natural gas, and power are all showing an increasing trend. Among them, the increase of natural gas production is as high as 38,947,730 tons of standard coal. However, the proportions of coal, natural gas, and power change inconsistently, where the proportions of coal and natural gas increase while that of power decreases. Corn production accounts for more than 80% of the total, which is in the eldest brother position in the food industry structure. Besides, there are differences between the planned values and optimal values of decision variables. Finally, suggestions are put forward to improve the sustainable development of water–energy–food in Inner Mongolia.
Dry Two-Step Self-Assembly of Stable Supported Lipid Bilayers on Silicon Substrates
Authors: Cisternas Marcelo A. , Palacios-Coddou Francisca , Molina Sebastian , Retamal Maria Jose , Gomez-Vierling Nancy , Moraga Nicolas , Zelada Hugo , Soto-Arriaza Marco A. , Corrales Tomas P. , Volkmann Ulrich G.
Published: September 2020

Artificial membranes are models for biological systems and are important for applications. We introduce a dry two-step self-assembly method consisting of the high-vacuum evaporation of phospholipid molecules over silicon, followed by a subsequent annealing step in air. We evaporate dipalmitoylphosphatidylcholine (DPPC) molecules over bare silicon without the use of polymer cushions or solvents. High-resolution ellipsometry and AFM temperature-dependent measurements are performed in air to detect the characteristic phase transitions of DPPC bilayers. Complementary AFM force-spectroscopy breakthrough events are induced to detect single- and multi-bilayer formation. These combined experimental methods confirm the formation of stable non-hydrated supported lipid bilayers with phase transitions gel to ripple at 311.5 ± 0.9 K, ripple to liquid crystalline at 323.8 ± 2.5 K and liquid crystalline to fluid disordered at 330.4 ± 0.9 K, consistent with such structures reported in wet environments. We find that the AFM tip induces a restructuring or intercalation of the bilayer that is strongly related to the applied tip-force. These dry supported lipid bilayers show long-term stability. These findings are relevant for the development of functional biointerfaces, specifically for fabrication of biosensors and membrane protein platforms. The observed stability is relevant in the context of lifetimes of systems protected by bilayers in dry environments.
Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application
Authors: Szilágyi Melinda , Pös Ondrej , Márton Éva , Buglyó Gergely , Soltész Beáta , Keserű Judit , Penyige András , Szemes Tomas , Nagy Bálint
Published: September 2020

Liquid biopsy recently became a very promising diagnostic method that has several advantages over conventional invasive methods. Liquid biopsy may serve as a source of several important biomarkers including cell-free nucleic acids (cf-NAs). Cf-DNA is widely used in prenatal testing in order to characterize fetal genetic disorders. Analysis of cf-DNA may provide information about the mutation profile of tumor cells, while cell-free non-coding RNAs are promising biomarker candidates in the diagnosis and prognosis of cancer. Many of these markers have the potential to help clinicians in therapy selection and in the follow-up of patients. Thus, cf-NA-based diagnostics represent a new path in personalized medicine. Although several reviews are available in the field, most of them focus on a limited number of cf-NA types. In this review, we give an overview about all known cf-NAs including cf-DNA, cf-mtDNA and cell-free non-coding RNA (miRNA, lncRNA, circRNA, piRNA, YRNA, and vtRNA) by discussing their biogenesis, biological function and potential as biomarker candidates in liquid biopsy. We also outline possible future directions in the field.
Stem Cells Secretome from Oral Tissue Could Represent a Promising Therapeutic Approach in COVID-19-Disease?
Authors: Diomede Francesca , Marconi Guya D. , Fonticoli Luigia , Pizzicannella Jacopo , Trubiani Oriana
Published: September 2020

At present, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection has quickly become a health emergency because no specifics vaccines or drugs, at this moment, are available. Recent studies have shown that the transplantation of mesenchymal stem cells (MSCs) into Coronavirus Disease 2019 (COVID-19) patients could represent a promising strategy for the development of new therapeutic methods. We speculate and suggest that the secretome of human Oral Tissue Stem Cells (hOTSCs), for their immunomodulatory and anti-inflammatory specific properties, could exert beneficial effects on the COVID-19 patients through an innovative aerosolisation technique. This non-invasive technique can offer multiple advantages in prophylaxis, as well as the prevention and treatment of severe epidemic respiratory syndrome with minimum risk and optimal therapeutic effects. This has the potential to create a novel pathway towards immunomodulatory therapy for the treatment of COVID-19 positive patients.
Anti-Telomerase CD4+ Th1 Immunity and Monocytic-Myeloid-Derived-Suppressor Cells Are Associated with Long-Term Efficacy Achieved by Docetaxel, Cisplatin, and 5-Fluorouracil (DCF) in Advanced Anal Squamous Cell Carcinoma: Translational Study of Epitopes-HPV01 and 02 Trials
Authors: Spehner Laurie , Kim Stefano , Vienot Angélique , François Eric , Buecher Bruno , Adotevi Olivier , Vernerey Dewi , Abdeljaoued Syrine , Meurisse Aurélia , Borg Christophe
Published: September 2020

Docetaxel, cisplatin and 5-fluorouracil (DCF) chemotherapy regimen is highly effective in advanced anal squamous cell carcinoma (SCCA), as demonstrated by the Epitopes-HPV02 study results. Here, we analyzed the impact of DCF regimen and the prognostic value of adaptive immune responses and immunosuppressive cells in SCCA patients included in two prospective studies (Epitopes-HPV01 and HPV02). The presence of T-cell responses against Human papillomavirus (HPV)16-E6/E7 and anti-telomerase (hTERT)-antigens was measured by IFNᵧ-ELISpot. Here, we showed that HPV-adaptive immune responses are increased in SCCA patients. SCCA patients also displayed enhanced circulating TH1 T-cells restricted by hTERT. Exposition to DCF increased hTERT immunity but not HPV or common viruses immune responses. Notably, the correlation of hTERT immune responses with SCCA patients’ clinical outcomes highlights that hTERT is a relevant antigen in this HPV-related disease. The influence of peripheral immunosuppressive cells was investigated by flow cytometry. While both regulatory T-cells and monocytic-myeloid-derived suppressive cells (M-MDSC) accumulated in the peripheral blood of SCCA patients, only high levels of M-MDSC were negatively correlated with hTERT adaptive immune responses and predicted poor prognosis. Altogether, our results reveal that hTERT is a relevant antigen in HPV-driven SCCA disease and that M-MDSC levels influence TH1-adaptive immune responses and patients’ survival.
Mitochondrial Surveillance by Cdc48/p97: MAD vs. Membrane Fusion
Authors: Escobar-Henriques Mafalda , Anton Vincent
Published: September 2020

Cdc48/p97 is a ring-shaped, ATP-driven hexameric motor, essential for cellular viability. It specifically unfolds and extracts ubiquitylated proteins from membranes or protein complexes, mostly targeting them for proteolytic degradation by the proteasome. Cdc48/p97 is involved in a multitude of cellular processes, reaching from cell cycle regulation to signal transduction, also participating in growth or death decisions. The role of Cdc48/p97 in endoplasmic reticulum-associated degradation (ERAD), where it extracts proteins targeted for degradation from the ER membrane, has been extensively described. Here, we present the roles of Cdc48/p97 in mitochondrial regulation. We discuss mitochondrial quality control surveillance by Cdc48/p97 in mitochondrial-associated degradation (MAD), highlighting the potential pathologic significance thereof. Furthermore, we present the current knowledge of how Cdc48/p97 regulates mitofusin activity in outer membrane fusion and how this may impact on neurodegeneration.
Wound Healing and Cell Dynamics Including Mesenchymal and Dental Pulp Stem Cells Induced by Photobiomodulation Therapy: An Example of Socket-Preserving Effects after Tooth Extraction in Rats and a Literature Review
Authors: Daigo Yuki , Daigo Erina , Fukuoka Hiroshi , Fukuoka Nobuko , Ishikawa Masatsugu , Takahashi Kazuya
Published: September 2020

High-intensity laser therapy (HILT) and photobiomodulation therapy (PBMT) are two types of laser treatment. According to recent clinical reports, PBMT promotes wound healing after trauma or surgery. In addition, basic research has revealed that cell differentiation, proliferation, and activity and subsequent tissue activation and wound healing can be promoted. However, many points remain unclear regarding the mechanisms for wound healing induced by PBMT. Therefore, in this review, we present an example from our study of HILT and PBMT irradiation of tooth extraction wounds using two types of lasers with different characteristics (diode laser and carbon dioxide laser). Then, the effects of PBMT on the wound healing of bone tissues are reviewed from histological, biochemical, and cytological perspectives on the basis of our own study of the extraction socket as well as studies by other researchers. Furthermore, we consider the feasibility of treatment in which PBMT irradiation is applied to stem cells including dental pulp stem cells, the theme of this Special Issue, and we discuss research that has been reported on its effect.
FGFR Fusions in Cancer: From Diagnostic Approaches to Therapeutic Intervention
Authors: De Luca Antonella , Esposito Abate Riziero , Rachiglio Anna Maria , Maiello Monica Rosaria , Esposito Claudia , Schettino Clorinda , Izzo Francesco , Nasti Guglielmo , Normanno Nicola
Published: September 2020

Fibroblast growth factor receptors (FGFRs) are tyrosine kinase receptors involved in many biological processes. Deregulated FGFR signaling plays an important role in tumor development and progression in different cancer types. FGFR genomic alterations, including FGFR gene fusions that originate by chromosomal rearrangements, represent a promising therapeutic target. Next-generation-sequencing (NGS) approaches have significantly improved the discovery of FGFR gene fusions and their detection in clinical samples. A variety of FGFR inhibitors have been developed, and several studies are trying to evaluate the efficacy of these agents in molecularly selected patients carrying FGFR genomic alterations. In this review, we describe the most frequent FGFR aberrations in human cancer. We also discuss the different approaches employed for the detection of FGFR fusions and the potential role of these genomic alterations as prognostic/predictive biomarkers.
Clearing of Foreign Episomal DNA from Human Cells by CRISPRa-Mediated Activation of Cytidine Deaminases
Authors: Brezgin Sergey , Kostyusheva Anastasiya , Ponomareva Natalia , Volia Viktoriia , Goptar Irina , Nikiforova Anastasiya , Shilovskiy Igor , Smirnov Valery , Kostyushev Dmitry , Chulanov Vladimir
Published: September 2020

Restriction of foreign DNA is a fundamental defense mechanism required for maintaining genomic stability and proper function of mammalian cells. APOBEC cytidine deaminases are crucial effector molecules involved in clearing pathogenic DNA of viruses and other microorganisms and improperly localized self-DNA (DNA leakages). Mastering the expression of APOBEC provides the crucial means both for developing novel therapeutic approaches for combating infectious and non-infectious diseases and for numerous research purposes. In this study, we report successful application of a CRISPRa approach to effectively and specifically overexpress APOBEC3A and APOBEC3B deaminases and describe their effects on episomal and integrated foreign DNA. This method increased target gene transcription by >6–50-fold in HEK293T cells. Furthermore, CRISPRa-mediated activation of APOBEC3A/APOBEC3B suppressed episomal but not integrated foreign DNA. Episomal GC-rich DNA was rapidly destabilized and destroyed by CRISPRa-induced APOBEC3A/APOBEC3B, while the remaining DNA templates harbored frequent deaminated nucleotides. To conclude, the CRISPRa approach could be readily utilized for manipulating innate immunity and investigating the effects of the key effector molecules on foreign nucleic acids.
Study on Physicochemical and Thermal Properties of Tetrabutylammonium-Based Cation Ionic Salts Induced by Al2O3 Additive for Thermal Energy Storage Application
Authors: Sutjahja Inge M. , Wonorahardjo Surjani , Wonorahardjo Surjamanto
Published: September 2020

The physicochemical and thermal properties of tetrabutylammonium bromide (TBA-Br) and tetrabutylammonium hexafluorophosphate (TBA-PF6), and their change with the addition of Al2O3, were investigated using infrared (IR) spectroscopy and by simultaneously conducting thermal thermogravimetric (TG) analysis and differential thermal analysis (DTA) to obtain the differential scanning calorimetry (DSC) thermogram. The change in the IR data is characterized by the growth of a large peak in the range of 3500 cm−1 and the reduction of peaks below 1000 cm−1 with the additive concentration. The decomposition temperature determined from the peak in the DTG curve is nearly constant for TBA-Br, and it decreases with the addition of Al2O3 for TBA-PF6, although it does not depend on the concentration of the additives. The DTA curve of ionic salts with the addition of Al2O3 shows additional peaks, which indicates a change in the sample’s temperature at disorder or phase transitions. The variation in the melting temperature with additive concentration is similar to that of decomposition temperature. The maximum heat of fusion value was approximately 67 kJ kg−1 for the doped TBA-Br and TBA-PF6 but was achieved at a different additive concentration. This is due to the additional disorder in the system induced by the dissolution of Al2O3.
Assessing the Physiological Relevance of Cough Simulators for Respiratory Droplet Dispersion
Authors: Patel Shiv H. , Yim Wonjun , Garg Anupam K. , Shah Sahil H. , Jokerst Jesse V. , Chao Daniel L.
Published: September 2020

Various breathing and cough simulators have been used to model respiratory droplet dispersion and viral droplets, in particular for SARS-CoV-2 modeling. However, limited data are available comparing these cough simulations to physiological breathing and coughing. In this study, three different cough simulators (Teleflex Mucosal Atomization Device Nasal (MAD Nasal), a spray gun, and GloGermTM MIST) that have been used in the literature were studied to assess their physiologic relevance. Droplet size, velocity, dispersion, and force generated by the simulators were measured. Droplet size was measured with scanning electron microscopy (SEM). Slow-motion videography was used to 3D reconstruct and measure the velocity of each simulated cough. A force-sensitive resistor was used to measure the force of each simulated cough. The average size of droplets from each cough simulator was 176 to 220 µm. MAD Nasal, the spray gun, and GloGermTM MIST traveled 0.38 m, 0.89 m, and 1.62 m respectively. The average velocities for the MAD Nasal, spray gun, and GloGermTM MIST were 1.57 m/s, 2.60 m/s, and 9.27 m/s respectively, and all yielded a force of <0.5 Newtons. GloGermTM MIST and the spray gun most closely resemble physiological coughs and breathing respectively. In conclusion, none of the simulators tested accurately modeled all physiologic characteristics (droplet size, 3-D dispersion velocity, and force) of a cough, while there were various strengths and weaknesses of each method. One should take this into account when performing simulations with these devices.
ICU-Acquired Hypernatremia Is Associated with Persistent Inflammation, Immunosuppression and Catabolism Syndrome
Authors: Rugg Christopher , Ströhle Mathias , Treml Benedikt , Bachler Mirjam , Schmid Stefan , Kreutziger Janett
Published: September 2020

Developing hypernatremia while on intensive care unit (ICU) is a common problem with various undesirable effects. A link to persistent inflammation, immunosuppression and catabolism syndrome (PICS) can be established in two ways. On the one hand, hypernatremia can lead to inflammation and catabolism via hyperosmolar cell stress, and on the other, profound catabolism can lead to hypernatremia via urea-induced osmotic diuresis. In this retrospective single-center study, we examined 115 patients with prolonged ICU stays (≥14 days) and sufficient renal function. Depending on their serum sodium concentrations between ICU day 7 and 21, allocation to a hypernatremic (high) and a nonhypernatremic group (low) took place. Distinct signs of PICS were detectable within the complete cohort. Thirty-three of them (28.7%) suffered from ICU-acquired hypernatremia, which was associated with explicitly higher signs of inflammation and ongoing catabolism as well as a prolonged ICU length of stay. Catabolism was discriminated better by the urea generation rate and the urea-to-creatinine ratio than by serum albumin concentration. An assignable cause for hypernatremia was the urea-induced osmotic diuresis. When dealing with ICU patients requiring prolonged treatment, hypernatremia should at least trigger thoughts on PICS as a contributing factor. In this regard, the urea-to-creatinine ratio is an easily accessible biomarker for catabolism.
Cracd Marks the First Wave of Meiosis during Spermatogenesis and Is Mis-Expressed in Azoospermia Mice
Authors: Snider Paige L. , Simmons Olga , Conway Simon J.
Published: September 2020

Testicular development starts in utero and maturation continues postnatally, requiring a cascade of gene activation and differentiation into different cell types, with each cell type having its own specific function. As we had previously reported that the Capping protein inhibiting regulator of actin (Cracd) gene was expressed in the adult mouse testis, herein we examine when and where the β-catenin associated Cracd is initially expressed during postnatal testis development. Significantly, Cracd mRNA is present in both the immature postnatal and adult testis in round spermatid cells, with highest level of expression occurring during the first wave of meiosis and spermatogenesis. In the juvenile testes, Cracd is initially expressed within the innermost region but as maturation occurs, Cracd mRNA switches to a more peripheral location. Thereafter, Cracd is downregulated to maintenance levels in the haploid male germ cell lineage. As Cracd mRNA was expressed within developing round spermatids, we tested its effectiveness as a biomarker of non-obstructive azoospermia using transgenic knockout mice models. Meaningfully, Cracd expression was absent in Deleted in azoospermia like (Dazl) null testis, which exhibit a dramatic germ cell loss. Moreover, Cracd was abnormally regulated and ectopically mis-expressed in Polypyrimidine tract binding protein-2 (Ptbp2) conditional germ cell restricted knockout testis, which exhibit a block during spermatid differentiation and a reduction in the number of late stage spermatocytes coincident with reduced β-catenin expression. Combined, these data suggest that Cracd is a useful first wave of spermatogenesis biomarker of azoospermia phenotypes, even prior to an overt phenotype being evident.
Accuracy in Judging Others’ Personalities: The Role of Emotion Recognition, Emotion Understanding, and Trait Emotional Intelligence
Authors: Jaksic Cyril , Schlegel Katja
Published: September 2020

The ability to accurately judge others’ personality and the ability to accurately recognize others’ emotions are both part of the broader construct of interpersonal accuracy (IPA). However, little research has examined the association between these two IPA domains. Little is also known about the relationship between personality judgment accuracy and other socio-emotional skills and traits. In the present study, 121 participants judged eight traits (Big Five, intelligence, cooperativeness, and empathy) in each of 30 targets who were presented either in a photograph, a muted video, or a video with sound. The videos were 30 second excerpts from negotiations that the targets had engaged in. Participants also completed standard tests of emotion recognition ability, emotion understanding, and trait emotional intelligence. Results showed that personality judgment accuracy, when indexed as trait accuracy and distinctive profile accuracy, positively correlated with emotion recognition ability and was unrelated to emotion understanding and trait emotional intelligence. Female participants were more accurate in judging targets’ personality than men. These results provide support for IPA as a set of correlated domain-specific skills and encourage further research on personality judgment accuracy as a meaningful individual difference variable.