bluuebunny/crossref_metadata_2025 huggingface dataset:
Peningkatan Keterampilan Proses Sains Melalui Kegiatan Laboratorium Disertai Strategi Catatan Terbimbing (Guided Note Taking) Pada Siswa Kelas VIII E SMP Negeri 7 Surakarta Tahun Pelajaran 2010/2011
Authors: Nugroho Anggita Widya , Indrowati Meti , Sugiharto Bowo
Published: March 2021

The purposes of this research is to improve the science process skills through applying laboratory activity with guided note taking to the students of VIII E class at SMP Negeri 7 Surakarta, academic year 2010/2011. This research refers to The Classroom Action Research which is conducted in 2 cycles that consist of planning, action, observing, and reflecting. The subject of this research is 36 students of VIII E class at SMP Negeri 7 Surakarta, academic year 2010/2011. The sources of the data consist of information from teachers and students, places and events that happened during the process of teaching and learning, and also some documents/files. The data of this research is collected by using observation sheet, questionnaire, interview, and documentations. The data is analyzed in qualitative descriptive method. The validity of data is tested through method and observer triangulation. The result of this research shows that has done in two cyrcles can be concluded that the application of laboratory activity with guided note taking can improve the science process skills in observe, clasificate, predict, measure, conclude, and communicate skills of the student of VIII E class at SMP Negeri 7 Surakarta, academic year 2010/2011.

 

Key Words: Science Process Skills, Laboratory Activity, Guided Note Taking
Penerapan Model Pembelajaran Reflektif pada Pembelajaran Biologi terhadap Hasil Belajar Siswa Kelas X SMA Negeri Colomadu Tahun Pelajaran 2012/2013
Authors: Prasetyo Abdi , Santosa Slamet , Marjono Marjono
Published: March 2021

The purpose of the research is to know the influence application of Refelective Learning model toward biology learning achievement of tenth degree students at SMA Negeri Colomadu in academic year 2012/ 2013. This research was Quasi Experiment research which used Posttest Nonequivalent Control Group Design. This research applyed Reflective Learning model in experimental group and Direct Instruction Learning model in control group. Population’s research is the entire class tenth at SMA Negeri Colomadu in academic year 2012/2013.  Sampling technique used Cluster Sampling, result has chosen tenth 5 as experiment group and  tenth 4 as control group.  Data was collected using  document, observation sheet, and multiple choice test. The data were analyzed by t-test. Result of the research in biology learning achievement between experiment and control group have real differents in cognitive, psychomotor, and affective domain. The conclusion that application of Refelective Learning model has taken good effect toward biology learning achievement of tenth degree students at SMA Negeri  Colomadu in academic year 2012/2013.

 

 

Key Words: Refelective Learning Model,  Learning Achievement
Peningkatan Kemampuan Berpikir Kritis dan Hasil Belajar Biologi Siswa Kelas VII-A Melalui Penerapan Model Guided Inquiry dengan Bantuan Social Network Facebook di SMPN 1 Kartasura
Authors: Fanani Fildza Hilfi , Probosari Riezky Maya , Sudarisman Suciati
Published: March 2021

This study is aimed at improving the students’ critical thinking ability and achievement in Biology of VII A class of SMPN 1 Kartasura through the implementation of guided inquiry model assisted by the use of facebook. This research is a classroom action research involving  several cycles in which each cycle includes four stages those are planning, action, observation, and reflection. The subject of this research is the students of VII A class at SMPN 1 Kartasura in academic year of 2012/2013. The data are obtained through the test (essay and multiple-choice) and non-test (observation, interviews and questionnaires). The data are analyzed through descriptive analytical techniques and validated through triangulation techniques.  Critical thinking ability consists of six aspects those are interpretation, analysis, evaluation, inference, explanation, and self-regulation. The average improvement percentage of critical thinking ability test in Pre-cycle, Cycle I, Cycle II, and Cycle III sequently are (41,36%; 52,66%; 65,32%; 77,26%). Students’ achievement is viewed from the increase of student learning completeness consisting of cognitive, psychomotor, and affective. The result of percentage attainment average from cognitive aspects in Pre-cycle, Cycle I, Cycle II, and Cycle III sequently are (36,11%; 47,22%; 72,22%; 100%). Psychomotor students’ achievement consists of three aspects, those are stringing experimental tool, observing the object changes that occur in the experiment, and communicating the results of experiments and worksheets in the presentation. The result of percentage attainment average from psychomotor aspects in Cycle I, Cycle II, and Cycle III sequently are (64,12%; 81,25%; 90,66%). Affective students’ achievement includes three aspect which are being meticulous in observing object from results of the experiment, being discipline in collecting worksheets, and being cooperative in a group discussion. The result of percentage attainment average from affective aspects in Cycle I, Cycle II, and Cycle III sequently are (61,81%; 77,31%; 91,92%). Based on the results of the research it can be concluded that the implementation of guided inquiry model assisted by the use of facebook can improve the students’ critical thinking ability and achievement in Biology of VII A class of SMPN 1 Kartasura.

 

 

Key Words: Guided Inquiry, Facebook, Biology Critical Thinking Ability,  Students’ Achievement in Biology
PENGARUH MODEL DISCOVERY LEARNING DENGAN METODE LATERAL THINKING TERHADAP KEMAMPUAN BERTANYA SISWA KELAS X IPA SMAN 2 KARANGANYAR
Authors: Aurlena Weny Puput , Sudarisman Suciati , Ariyanto Joko
Published: March 2021

This research aimed to find out the effect of discovery learning with lateral thinking on questioning ability of the 10th IPA graders of SMAN 2 Karanganyar.This study was a quasi experimental research with post-test only with non-equivalent group research design. The population of research was all of the 10th graders of SMAN 2 Karanganyar. The sampling technique used was cluster sampling one. The 10th IPA 2 grade served as experiment group and the 10th IPA 3 as control. Techniques of collecting data used were test and non-test. Test technique employed essay question instrument to measure the learning outcome in cognitive domain, while non-test technique used observation sheet instrument to measure the students’ questioning ability, learning outcome in psychomotor and affective domains and the implementation of learning syntax. Hypothesis testing was carried out using t-test with SPSS version 17 help.The results showed that the ability to ask students between the experimental group and the control group were significantly different . Values experimental group students' ability to ask include: questions asking for an explanation of 1.54 ; The question of what , why and how 36.18 ; background hypothetical question 40.13 . Value KPS control groups included: the question asked for an explanation of 0.57 ; The question of what , why and how 41.03 ; background hypothetical question 29.06 This research concluded that there was an effect of discovery learning model with lateral thinking method on the questioning ability of the 10th IPA graders of SMAN 2 Karanganyar.

 

Keywords: discovery learning, lateral thinking, questioning ability
UPAYA MENINGKATKAN KETERAMPILAN PROSES SAINS DAN MENGURANGI MISKONSEPSI MELALUI PENERAPAN E-MODULE BERBASIS PROBLEM-BASED LEARNING KELAS X MIA 2 SMA BATIK 1 SURAKARTA
Authors: Wati Henny Purnama , Karyanto Puguh , Dwiastuti Sri , Wulandari Dynna Sri
Published: March 2021

This research aimed to improve the scientific process skills and reduce misconception through the application of E-Module based on Problem-Based Learning in the X MIA 2 graders of SMA Batik 1 Surakarta. This research was a Classroom Action Research consisting of 2 cycles. Each cycle consisted of four stages: planning, acting, observing, and reflecting. The subject of research was the X MIA 2 graders of SMA Batik 1 Surakarta consisting of 42 students. Techniques of collecting data used were multiple choice test and observation to measured scientific process skills, open-ended reasoning of Two-Tier Diagnostic Test to measure misconseption, and interveiw as the proponent data related learning process. The data were analyzed using descriptive qualitative technique consist of three component: data reduction, data presentation, and taking the conclusion. Data validation was carried out using triangulation method. Target of research was 20% to increased scientific process skills and 20%  to reduced misconseption at the end of the cycle. The result of research showed that the scientific process skills to able increased through the application of E-Module based on Problem-Based Learning in all aspects: observing of 29.83%, categorizing of 11.73%, predicting of 37.33%, interpreting of 30.71%, measuring of 35.66%, communicating of 32.39%, designing experiment of 30.04%, experimenting of 21.82%, asking question of 23.42%, hypothesizing of 36.73%, and applying concept of 42.58%. On the other side, the  result of research also showed that there was a decreased misconception in ecological concept: population of 51.31%, community of 43.73%, ecosystem of 39.36%, and science, environment, technology and community (salingtemas) ecology of 54.33%. The conclusion of this research described that there was an increasing scientific process skills of 28.96% and reducing misconception of 47.18% by used the application of E-Module based on Problem-Based Learning.

 

Keywords  : Scientific Process Skills, Misconception, E-Module based on Problem Based Learning.
PENGEMBANGAN SUBJECT SPECIFIC PEDAGOGY BERBASIS PROBLEM BASED LEARNING UNTUK PENGUATAN SIKAP PEDULI LINGKUNGAN SISWA KELAS VII SMP
Authors: Nugroho Wahyu Adhi , Karyanto Puguh , Nurmiyati Nurmiyati
Published: March 2021

This research considered as Research and Development. The reseach conducted from January to May 2016. The purpose of this research is to develope Subject Spesific Pedagogy (SSP) based on Problem Based Learning (PBL) to reinforce student’s environmental-caring attitude in Grade VII SMP Negeri A Karanganyar academic year 2015/2016. The population of this research was of class VII in SMP N A Karanganyar. Class VII H and VII I were selested as the sample of research. The research design is 4D (four-D) by Thiagarajan. This research model consist of define, design, and develope. The disseminate phase was excluded in this research because the purpose of this research only develope product SSP based on PBL. The characteristic of product is integration of PBL syntax and enviromental subject. The result of validation from expert judgements showed that 90% in RPP (valid), 90% in module (valid), 90% in LKS (valid), and 83% in instrument of evaluation (valid). The development testing result from teacher and students showed that 84% and 79% in modul and LKS (valid). SSP based on PBL is effective to improve environmental-caring attitude. NEP data showed that the mean value of all aspect had been improved from 48,39% up to 54,86%. Paired sample’s t-test showed that sig=0,000 < α=0,05 it’s mean there was differences students environmental-caring attitude between pre and post-treatment. The conclusion of this this research showed that SSP based on PBL is valid and proper for Grade VII SMP N A Karanganyar.

 

Keywords : Subject Spesific Pedagogy, Problem Based Learning, Environmental-Caring Attitude, New Ecological Paradigm
PENERAPAN MODEL KONSTRUKTIVIS-METAKOGNITIF PADA MATERI SISTEM KOORDINASI UNTUK MENINGKATKAN KEMAMPUAN BERPIKIR KRITIS SISWA KELAS XI MIA 1 SMA NEGERI 6 SURAKARTA TAHUN PELAJARAN 2015/2016
Authors: Anggraeni Yunita Nur , Prayitno Baskoro Adi , Ariyanto Joko
Published: March 2021

This study aims to improving critical thinking skills of student of class XI MIA 1 SMA Negeri 6 Surakarta in the academic year 2015/2016 through the implementation of Constructivist-Metacogitive model. This research considered as Classroom Action Research that performed whitin 2 cycles and consist of 4 steps namely planning, action, observation, and reflection. The research procedure refers to Kemmis-Mc.Taggart spiral technique. Data are obtained by questionnaire, essay test, interview, and documentation. Data analysis using qualitative descriptive technique. The main data regarding students' critical thinking skills covering several aspects, namely the interpretation, analysis, evaluation, inference, explanation and self-regulation was measured using a test arranged by the researcher. Data analysis using descriptive analysis technique. Data validation using triangulation techniques. The average yield of pre-cycle critical thinking skills is 33.71% (low), then increased to 47.6% (average) at the first cycle, and increased to 75.76% (high) at the second cycle. The student’s critical thingking skill improvement have reached the reseach target (≥20%), Thus, application of constructivist-metacognitive models can improve students' critical thinking skills. The results showed that the application of Constructivist Model-metacognitive improving critical thinking skills of the students of class XI MIA 1 SMA Negeri 6 Surakarta.

Keywords: Constructivis-metacognitive models, Critical Thinking Skills, Coordination System.
Penerapan Model Pembelajaran Kooperatif Tipe NHT Berbantuan Alat Peraga Sistem Pernapasan Manusia
Authors: Uki Nonci M.
Published: March 2021

Hasil observasi di beberapa sekolah di Kabupaten Timor Tengah Selatan (TTS) menunjukkan bahwa hasil belajar yang diperoleh siswa masih di bawah kriteria ketuntasan minimum (KKM). Hal ini disebabkan karena keterbatasan kreativitas guru dalam memilih metode yang tepat dalam proses pembelajaran, dan masih banyak guru yang belum memanfaatkan media pembelajaran sehingga siswa merasa bosan dan pada akhirnya berpengaruh pada hasil belajar siswa. Penelitian ini bertujuan untuk Menganalisis keefektifan model pembelajaran kooperatif tipe NHT dan Konvensional berbantuan alat peraga terhadap hasil belajar siswa pada materi sistem pernapasan manusia. Metode penelitian yang digunakan adalah Quasi Experiment dengan desain Nonequivalent Control Group Design yang melibatkan dua kelas, yaitu kelas yang menggunakan model pembelajaran  NHT dan Konvensional. Data dikumpulkan melalui tes hasil belajar dan dianalisis menggunakan uji N-Gain. Hasil penelitian menunjukkan bahwa rata-rata hasil belajar kelompok NHT (0,70) dan Konvensional (0,57). Penggunaan model pembelajaran kooperatif tipe NHT berbantuan alat peraga lebih efektif meningkatkan hasil belajar siswa dibandingkan model Konvensional
Peningkatan Kemampuan Berpikir Kreatif Melalui Penerapan Project Based Learning Pada Materi Pencemaran dan Daur Ulang Limbah
Authors: Nugroho Gilang Akbar , Prayitno Baskoro Adi , Ariyanto Joko
Published: March 2021

This study aims to improve the ability of creative thinking through Project Based Learning on pollution and waste recycling materials. This research is a classroom action research conducted for 2 cycles. Each research cycle consists of 4 stages, namely planning, action, observation, and reflection. The subjects of the study were the students of grade X SMA N 2 Boyolali with 37 students. Research data obtained through class observation, interviews, documentation, and tests of creative thinking ability that consists of aspects of fluency, flexibility, originality, and elaboration. Validation of data is done by triangulation technique. Data analysis using qualitative descriptive analysis technique. The results showed that each aspect of creative thinking ability in pre-cycle that is fluency 25,4% included in category less, flexibility 24,8% included in category less, originality 15,9% included in category less, and elaboration 18,6 % Included in the category less. The achievement percentage of each aspect of the ability of creative thinking in the first cycle that is fluency 34.5% included in the category enough, flexibility 41.8% included in the category enough, originality 37% included in the category enough, and elaboration 40.5% included in the category enough. The achievement percentage of each aspect of creative thinking ability in cycle II that is fluency 78,6% included in creative category, flexibility 52,1% included in enough category, 48,9% originality included in enough category, and elaboration 58,6% including In sufficient category. The achievement of students' creative thinking ability from pre-cycle to cycle II has increased according to the research target of ≥20% through the implementation of Project Based Learning model
PENGARUH MODEL PEMBELAJARAN BLENDED LEARNING MELALUI MEDIA GAMBAR UNTUK MENINGKATKAN HASIL BELAJAR SISWA KELAS VIII SMP NEGERI 1 BRONDONG TAHUN PELAJARAN 2017/2018
Authors: Banu Mariana Wasti Enggelina , Mawartiningsih Lilik
Published: March 2021

Education is the most important in human life especially with the development of science and technology as a supporter in education. The main problem faced by the world of education is the low level of student learning outcomes. Based on the results of field observations obtained data, that the average score achieved in grade VIII students less than KKM. So need to do research by using blended learning model. This study aims to determine the effect of blended learning model of learning outcomes of students of SMP Negeri I Brondong academic year 2017/2018. This research belongs to Quasi experimental research by using pretest-post test. The instruments used in this study are pretest and posttest test. The data obtained from this research is then analyzed using t-test. Based on the results of research on the effect of learning model of blended learning through the media of image on the students of class VIII SMPN I Brondong Lamongan Year 2017/2018 that is the influence of learning model blended learning through the media images of student learning outcomes on the subject of excretory system. The result of the research shows that there are significant differences in the results of the class biology study which are studied using the conventional model and the class that is taught using the blended learning model through the media of the picture in the VIII students of SMP Negeri I Brondong. The result of data analysis of student learning result showed that the average value of cognitive domain of experimental class is 76.2 while control class is 72,4%. From the analysis of data can be concluded the average value of the experimental class is higher than the control class. It is there influence the learning model blended learning through the media images of student learning outcomes.

 
PENGUKURAN TINGKAT PEMAHAMAN MAHASISWA TERHADAP VISI MISI PROGRAM STUDI PENDIDIKAN BIOLOGI FKIP UNTAN
Authors: Maulidi Andri , Syamswisna Syamswisna , Daningsih Entin , Ariyati Eka , Marlina Reni
Published: March 2021

The Implementation of education in Higher Education must have the vision and mission to achieve a certain goal. The result of the vision and mission statement is socialized to get feedback as an evaluation and perfection of the vision and mission. The achievement of vision and mission that have been determined must be known, understood, and implemented by all academicians including students. This research was conducted to measure the level of students' understanding of the vision and mission of Biology Education Study Program of FKIP Untan. This research used qualitative approach with descriptive study. The subjects in this research were 246 students of Biology Education Study Program of FKIP Untan class of 2015, 2016, and 2017. The research instrument used was a questionnaire with closed and open statement. The results of this study indicated that in general the students have understood the vision and mission well. Data analysis showed that 236 (95,93%) students have read vision and mission, 190 (77,24%) and 204 (82,93%) students knew about vision and mission, 203 (33,44%), 101 (64%) students were the highest percentage of students who read the vision and mission in standing banner or class and in academic guidance book. Furthermore, 162 students (65.85 %) agreed that they have been well informed about the vision and mission, 165 students (67.07%) agreed that they have understood the vision and mission, 173 students (70.33%) agreed with the existence of vision and mission, and 159 students (64.63%) agreed that the vision and mission were used as references.
Congratulations! The Best Paper Award 2015
Authors: Office Editorial
Published: March 2021

The sixth Best Paper Award 2015 ceremony was held in Kyoto, Japan, on October 20 in the 8thsup> International Conference on Leading Edge Manufacturing in 21st Century (LEM21) and the certificate with a prize JPY100,000 was given to the Best Paper Award 2015 winner, Prof. Konrad Wegener. The Best Paper was severely selected from among 88 papers published in Vol.8 (2014). We congratulate the winner and sincerely wish for his future success.

 

Title: Thermally Caused Location Errors of Rotary Axes of 5-Axis Machine Tools

Authors: Michael Gebhardt, Alexander Schneeberger, Sascha Weikert, Wolfgang Knapp, and Konrad Wegener

Int. J. of Automation Technology Vol.8, No.4 pp. 511-522, 2014
An Improved Algorithm for Detection and Pose Estimation of Texture-Less Objects
Authors: Peng Jian , Su Ya ,   School of Automation, China University of Geosciences 388 Lumo Road, Hongshan, Wuhan, Hubei 430074, China,   Hubei Key Laboratory of Advanced Control and Intelligent Automation for Complex Systems 388 Lumo Road, Hongshan, Wuhan, Hubei 430074, China
Published: March 2021

This paper introduces an improved algorithm for texture-less object detection and pose estimation in industrial scenes. In the template training stage, a multi-scale template training method is proposed to improve the sensitivity of LineMOD to template depth. When this method performs template matching, the test image is first divided into several regions, and then training templates with similar depth are selected according to the depth of each test image region. In this way, without traversing all the templates, the depth of the template used by the algorithm during template matching is kept close to the depth of the target object, which improves the speed of the algorithm while ensuring that the accuracy of recognition will not decrease. In addition, this paper also proposes a method called coarse positioning of objects. The method avoids a lot of useless matching operations, and further improves the speed of the algorithm. The experimental results show that the improved LineMOD algorithm in this paper can effectively solve the algorithm’s template depth sensitivity problem.
A Fast Method for Fuzzy Rules Learning with Derivative-Free Optimization by Formulating Independent Evaluations of Each Fuzzy Rule
Authors: Uehara Kiyohiko , Hirota Kaoru ,   Ibaraki University 4-12-1 Nakanarusawa-cho, Hitachi, Ibaraki 316-8511, Japan,   Beijing Institute of Technology No.5 South Street, Zhongguancun, Haidian District, Beijing 100081, China
Published: March 2021

A method is proposed for evaluating fuzzy rules independently of each other in fuzzy rules learning. The proposed method is named α-FUZZI-ES (α-weight-based fuzzy-rule independent evaluations) in this paper. In α-FUZZI-ES, the evaluation value of a fuzzy system is divided out among the fuzzy rules by using the compatibility degrees of the learning data. By the effective use of α-FUZZI-ES, a method for fast fuzzy rules learning is proposed. This is named α-FUZZI-ES learning (α-FUZZI-ES-based fuzzy rules learning) in this paper. α-FUZZI-ES learning is especially effective when evaluation functions are not differentiable and derivative-based optimization methods cannot be applied to fuzzy rules learning. α-FUZZI-ES learning makes it possible to optimize fuzzy rules independently of each other. This property reduces the dimensionality of the search space in finding the optimum fuzzy rules. Thereby, α-FUZZI-ES learning can attain fast convergence in fuzzy rules optimization. Moreover, α-FUZZI-ES learning can be efficiently performed with hardware in parallel to optimize fuzzy rules independently of each other. Numerical results show that α-FUZZI-ES learning is superior to the exemplary conventional scheme in terms of accuracy and convergence speed when the evaluation function is non-differentiable.
Fuentes Mares y la filosofía de la historia (Sesión inicial del Seminario de Lectura de Textos Filosóficos Mexicanos)
Authors: Ordóñez Burgos Jorge
Published: March 2021

Fuentes Mares and the Philosophy of History (Opening session for the Seminar of Mexican Philosophical Texts Reading). Universitary teaching is a scholar activities zone, that recives low care, depreciating its value to contribuite the enrichment of scientific and humanistic investigations. The base of this article is the lectio inauguralis manuscript of Seminar, modificated as philosophcal essay to show reflections on Philosophy of History by Fuentes Mares maked. The text analizes novels, tales, theatrical plays, historical monographies and philosophical books.
LOS Actos de Oposición en la Disidencia Magisterial en Ciudad Juárez
Authors: Ferman Avila Oliverio Ismael
Published: March 2021

El capítulo aborda, de manera breve, el producto de una investigación sobre los procesos que vive la disidencia magisterial en Ciudad Juárez, particularmente el movimiento RESISSSTE. Esta investigación se realizó en el marco del enfoque cualitativo y con un diseño metodológico fenomenográfico. Para su realización, las indagaciones iniciaron un martes del mes de mayo en 2016 y se extendieron por lapso de catorce meses para finalizar en el mes de Julio de 2017. Este artículo pretende ser un acercamiento al movimiento RESISSSTE desde una perspectiva política e ideológica, que busca describir la naturaleza política de los actos contestatarios de la disidencia magisterial en Ciudad Juárez. El marco teórico referente partió de los aportes de la pedagogía crítica, las teorías de la reproducción y la teoría de la resistencia. Este acercamiento implica por supuesto, un análisis de los anclajes ideológicos, experiencias de vida –tanto personal como profesional- de docentes que participan en la disidencia magisterial organizada, pero particularmente orientado a diferenciar, tal como lo supone McLaren (1998), entre las conductas de oposición y los mecanismos de resistencia que se delinean y ponen en práctica en la disidencia magisterial ante las aspectos torales que planteó la Reforma Educativa Peñista, entre estos: la formación y regulación del profesional docente –particularmente la evaluación y sus efectos en la estabilidad laboral-, y la implementación de un nuevo currículum, con las implicaciones normativas que implícita o explícitamente le subyacieron.
Educación no formal de los Rarámuri de la Alta Tarahumara
Authors: Escudero González Raquel Adilene
Published: March 2021

Este trabajo pretende resaltar la importancia de la educación no formal basada en la cosmovisión Rarámuri, aunada a la inclusión dentro del sistema educativo formal la cual aporta nuevos significados al proceso enseñanza aprendizaje tomando como base su identidad, contexto histórico, creencias y costumbres indígenas con el fin de que el docente actúe en consecuencia, desarrollando habilidades y competencias interculturales que mejoren el proceso de enseñanza - aprendizaje intercultural.
COVID-19 VACCINE: THE CHALLENGE OF HERBAL MEDICINE COMMUNITY BELIEF IN A DEVELOPING COUNTRY
Authors: Makrufardi Firdian , Saputri Ade , Phillabertha Paulin Surya
Published: March 2021

The first case of COVID-19 was officially confirmed by Indonesian government on the last March 2020. In fact, traditional or herbal medicine have a big influence on people’s decisions about their health. Hereby, the information about COVID-19 vaccine should get a place in peoples’ heart and mind.
A POTENTIAL ZOONOTIC PARASITE: CRYPTOSPORIDIUM PARVUM TRANSMISSION IN RATS, PIGS AND HUMANS IN WEST LOMBOK, INDONESIA.
Authors: Resnhaleksmana Ersandhi , Wijayanti Mahardika Agus , Artama Wayan Tunas
Published: March 2021

Background: Cryptosporidium is a neglected zoonotic disease, but with the , its incidence is increasing. Animals such as rats and pigs can act as intermediate hosts and transmit Cryptosporidium to humans due to their proximity. Transmission occurs due to the ability of Cryptosporidium to survive . The research aimed to identify and describe the transmission of Cryptosporidium from animals to humans. Materials and Methods: This research was a cross sectional study and samples were collected from caught in residential areas, 205 pigs, and 438 humans in West Lombok. Fecal samples were examined using polymerase chain reaction (PCR) and sequencing to isolate the presence of Cryptosporidium, and identify the genetic similarity of the parasites found in rats and pigs with those that infect humans. Results: The PCR results found Cryptosporidium parvum in 4.76% (4/84) in rats; 6.34% 13/205) in pigs; and 0.91% (4/438) in humans. The sequencing results showed genetic kinship of C. parvum in rats, pigs, and humans. Conclusion: There are genetic similarities of Cryptosporidium in animals and humans, requiring that the Public Health programs in those contaminated areas must receive priority attention to prevent further transmission of these potentially fatal parasites.
IMPROVING HEALTH PROTECTION STRATEGY OF THE NUCLEAR INDUSTRY ENTERPRISE PERSONNEL AND POPULATION LIVING WITHIN ITS CATCHMENT AREA
Authors:   Seversk Biophysical Research Center of the Federal Medical Biological Agency, Seversk, Russia, Kalinkin D.E. ,   Siberian State Medical University, Tomsk, Russia, Takhauov R.M. ,   Seversk Biophysical Research Center of the Federal Medical Biological Agency, Seversk, Russia,   Siberian State Medical University, Tomsk, Russia, Milto I.V. ,   Seversk Biophysical Research Center of the Federal Medical Biological Agency, Seversk, Russia,   Siberian State Medical University, Tomsk, Russia, Samoilova Yu.A. ,   Siberian Federal Scientific Clinical Center of the Federal Medical Biological Agency, Seversk, Russia, Zhuikova L.D. ,   Tomsk National Research Medical Center of the Russian Academy of Sciences, Russia, Takhauov A.R. ,   Seversk Biophysical Research Center of the Federal Medical Biological Agency, Seversk, Russia,   Siberian State Medical University, Tomsk, Russia, Takhauova L.R. ,   Seversk Biophysical Research Center of the Federal Medical Biological Agency, Seversk, Russia,   Siberian State Medical University, Tomsk, Russia
Published: March 2021

The purpose of the study is to develop approaches to improve the health protection strategy of the nuclear industry enterprise staff and population living within its catchment area in the context of the national project “Healthcare”. The material for the study was information on dynamics in population indicators characterizing health status of the nuclear industry enterprise staff and population living within its catchment area in 1970-2017. Results. The study shows that the analyzed population is characterized by a significant decrease in the birth rate, increased mortality (including people of working age) from diseases of the circulatory system, malignant neoplasms and external causes, population decline, and decreased life expectancy. Conclusion. On the basis of the results obtained, approaches have been developed to improve health protection strategy for the above contingents in the context of the National Project “Healthcare”.
WHICH HEALTH FINANCING MODEL IS BETTER: TAX-FINANCED OR SOCIAL HEALTH INSURANCE? WHAT DOES INTERNATIONAL EXPERIENCE PROVE?
Authors:   National Research University Higher School of Economics, Moscow, Russia, Potapchik E.G.
Published: March 2021

In Russia disputes on the need to abandon Compulsory Health Insurance (CHI) and return to the tax-based financing are yet to subside. At present, after the statement of the President of the Russian Federation V. Putin about the possibility to establish a state health care corporation, discussions on the issue have only escalated. Purpose. To conduct a comparative assessment of the public health financing model impact on the access and structural characteristics of health care delivery in the developed countries. Material and methods. Assessment of the potential impact of public funding models on the health system performance is carried out by analyzing variations in the main indicators of financial access, health care uptake and health status of the population, achieved in the developed countries with different health financing models. Results. Health care expenditures in countries with CHI are higher than in countries with the tax-based financing model. In countries with CHI the share of administrative expenses is slightly higher than in countries with the tax-based financing system. The share of spending on preventive care is slightly higher in countries with the tax-based financing system. There is a slightly lower level of outpatient and inpatient care uptake in countries with the tax-based financing system compared to countries with CHI. The premature mortality rate in countries with CHI is slightly lower than in countries with the tax-based system. Conclusion. The obtained data indicate that there are no significant differences in the access and structural characteristics of medical care in the health care system of the developed countries with different financing models. The main difference remains the level of health expenditures. In countries with CHI, the level of health expenditures is higher than in countries with the tax-based financing, which is largely due to the existence of a separate source of funding. The level of administrative costs in countries with CHI is also higher than in countries with the tax-based system.
Monitoring the Milwaukee Harbor breakwater : an Engineering With Nature® (EWN®) demonstration project
Authors: Geisthardt Eric , Suedel Burton , Janssen John
Published: March 2021

The US Army Corps of Engineers (USACE) maintains breakwaters in Milwaukee Harbor. USACE’s Engineering With Nature® (EWN®) breakwater demonstration project created rocky aquatic habitat with cobbles (10–20 cm) covering boulders (6–8 metric tons) along a 152 m section. A prolific population of Hemimysis anomala, an introduced Pontocaspian mysid and important food source for local pelagic fishes, was significantly (p < .05) more abundant on cobbles versus boulders. Food-habits data of alewife (Alosa pseudoharengus) and rainbow smelt (Osmerus mordax) provided evidence that H. anomala were a common prey item. Night surveys and gill netting confirmed O. mordax preferred foraging on the cobbles (p < .05) and consumed more H. anomala than at the reference site (p < .05). H. anomala comprised a significant portion of the diets of young-of-the-year (YOY) yellow perch (Perca flavescens), YOY largemouth bass (Micropterus salmoides), and juvenile rock bass (Ambloplites rupestris) caught on the breakwater. The natural features’ construction on the breakwater increased the available habitat for this benthopelagic macroinvertebrate and created a novel ecosystem benefiting forage fish and a nursery habitat benefiting nearshore game fish juveniles. These data will encourage the application of EWN concepts during structural repairs at other built navigation infrastructure.
Modernization and structural evaluation of the improved Overhead Cable System
Authors: Trim M. , Murray Matthew , Crane C.
Published: March 2021

A modernized Overhead Cable System prototype for a 689 ft (210 m) Improved Ribbon Bridge crossing was designed, assembled, and structurally tested. Two independent structural tests were executed, i.e., a component-level compression test of the BSS tower was performed to determine its load capacity and failure mode; and a system-level ‘dry’ test of the improved OCS prototype was conducted to determine the limit state and failure mode of the entire OCS. In the component-level compression test of the BSS tower, the compressive capacity was determined to be 102 kips, and the failure mode was localized buckling in the legs of the tower section. During system-level testing, the prototype performed well up to 40.5 kips of simulated drag load, which corresponds to a uniformly distributed current velocity of 10.7 ft/s. If a more realistic, less conservative parabolic velocity distribution is assumed instead, the drag load for an 11 ft/s current is 21.1 kips. Under this assumption, the improved OCS prototype has a factor of safety of 1.9, based on a 689-ft crossing and 11-ft/s current. The OCS failed when one of the tower guy wires pulled out of the ground, causing the tower to overturn.
Comparative genomic and secretomic characterisation of endophytic Bacillus velezensis LC1 producing bioethanol from bamboo lignocellulose
Authors: Hao Tang , Li Zheng , Yuanqiu Li , Lu Lei , Xiaowen Yang , Chaobing Luo
Published: March 2021

Bacillus is an excellent organic matter degrader, and it has exhibited various abilities required for lignocellulose degradation. Several B. velezensis strains encode lignocellulosases and however their usefulness based on their ability to transform biomass has not been appreciated. In the present study, genomic, comprehensive comparative genomic and secretomic analyses were used to clarify the lignocellulose-degrading potential of these bacteria. The complete genomes of 20 B. velezensis strains and an endophytic strain, B. velezensis LC1, were analysed to find common and unique genes encoding carbohydrate-active enzymes (CAZymes) and evaluate their potentials to degrade lignocellulose. By comparative whole genomic and CAZyomic analyses of all 21 strains, we identified genes coding for lignocellulolytic enzymes with the potential to degrade cellulose and hemicellulose. Further identification of the secretome of B. velezensis LC1 by liquid chromatography-tandem mass spectrometry (LC-MS/MS) confirmed that a considerable number of proteins in the culture medium are involved in lignocellulose degradation, including endoglucanase, hemicellulases, and other related proteins. Moreover, assays of the activities of several lignocellulolytic enzymes show that these enzymes are more active in bamboo powder compared to glucose substrates. After a 6-day treatment, the degradation efficiencies of cellulose, hemicellulose and lignin from bamboo powder were 59.90%, 75.44% and 23.41%, respectively. The hydrolysate was subjected to ethanol fermentation with Saccharomyces cerevisiae and Escherichia coli KO11, yielding 10.44 g/L ethanol after 96 h. These findings indicate that B. velezensis LC1, efficiently degrades bamboo lignocellulose components, allowing for subsequent ethanol production.
Differential Expression of Long Noncoding RNAs in Peripheral Blood Leukocytes of Patients with Schizophrenia
Authors: Wu Jie , Liu Zijun , Zhang Yunqiao , Teng Zhaowei , You Xu , Ruan Ye , Gao Changqing , Zeng Yong
Published: March 2021

Background: The diagnosis of schizophrenia (SCZ) depends on the evaluation of clinical symptoms, but there is no objective biomarker. Studies have shown that long non-coding RNAs (lncRNAs) may be involved in the pathogenesis of SCZ. In this study, we evaluated the differential gene expression of lncRNAs and mRNAs in peripheral blood (PBL) of patients with SCZ.Methods: We profiled the transcriptome of PBL in 50 patients with SCZ and 50 controls without psychiatric diagnoses, using RNA-seq. LncRNA-mRNA interactions were predicted using “RNAplex”, a hierarchical classification-Spielman correlation coefficient approach was used to analyze the correlation between lncRNAs and protein-coding gene expression among samples, and systematic bioinformatic methods (Go/Pathway) were used to perform lncRNA functional annotation. The results were validated using qPCR. Functional sites in sequences were predicted using the PROSITE, NCBI, UCSC, and JASPAR databases.Results: We identified 94 lncRNAs and 1179 mRNAs differentially expressed in PBL, of which 46 lncRNAs were identified for the first time. Enrichment of lncRNAs involved biological processes and signaling pathways related to neutrophil activation involved in the immune response. Spearman correlation coefficient analysis showed that 81 lncRNAs and 410 mRNAs had correlated expression (p < 0.01 and |r| ≥ 0.4). qPCR performed on independent samples verified that the core node of the lncRNA-mRNA co-expression network, the IL1RAP-TCONS_00138311 variable splicing transcript, was highly expressed in patients with SCZ (2△△Ct of 0.56, area under the ROC curve of 0.924). The top four ranked transcription factors were predicted to be HSF1, HSF2, HSF4, and FOXA1.Conclusions: Combined with sequence function analysis, we showed that the transcription factors FOXA1, HSF1, HSF2, and HSF4 may mediate the activation of IL1B-induced NF-kβ pathway and other inflammatory pathways through regulation of the IL1RAP alternative splicing transcript TCONS_00138311, thereby participating in the pathogenesis of SCZ. We propose that the frequency of differentially expressed lncRNAs in PBL may serve as a novel biomarker for diagnosis of SCZ.
Cytoplasmic Expression of DCLK1-S, a Novel DCLK1 Isoform, Is Associated with Tumor Aggressiveness and Worse Disease-Specific Survival in Colorectal Cancer
Authors: Kalantari Elham , Ghods Roya , Zanjani Leili Saeednejad , Rahimi Mandana , Eini Leila , Razmi Mahdieh , Asadi-Lari Mohsen , Madjd Zahra
Published: March 2021

Background: Oncogenic role of doublecortin-like kinase 1 (DCLK1) as a putative cancer stem cell (CSC) marker has been clarified in colorectal cancer (CRC). Isoform-specific function of DCLK1 has highlighted key role of the DCLK1-S (short isoform) in tumor maintenance, progression, and invasion. Considering the lack of commercial anti-DCLK1-S antibody suitable for immunohistochemical (IHC) application, this study was conducted to produce and validate an anti-DCLK1-S polyclonal antibody in order to specifically evaluate expression pattern and clinical significance of short isoform of DCLK1 in colorectal cancer tissues.Methods: Rabbit immunization was performed against a synthetic peptide corresponding to the published six specific amino acid sequences of DCLK1-S, and production of antibody was evaluated by enzyme-linked immunosorbent assay (ELISA). After IHC assessment of the purified anti-DCLK1-S polyclonal antibody, it was used to undertake a definitive study for determining prognostic significance of DCLK1-S expression in a well-deﬁned tissue microarray (TMA) series including 348 CRC and 51 adjacent normal tissues with a follow-up period of 108 months. Results: Positive immunoreactivity of DCLK1-S was found in 84.2% of CRC samples. Cytoplasmic expression was the main localization of DCLK1-S compared to nuclear and membranous area of tumor cells. Expression of DCLK1-S in CRC samples was significantly higher compared to adjacent normal samples (P <0.001). A positive significant association was found between high cytoplasmic expression of DCLK1-S and advanced tumor, nodes, and metastases (TNM) stage (P<0.001) as well as the increased tumor differentiation (P= 0.02). Moreover, the patients with CRC whose tumors showed higher cytoplasmic expression of DCLK1-S had worse disease-specific survival (DSS) (log-rank test, P = 0.03) and 5-year DSS rate (P= 0.01). Additionally, the improved prognostic value was seen in the patients with CRC with high DCLK1-S expression versus moderate expression (HR: 2.70, 95% CI: 0.98-7.38; p =0.04) by multivariate analysis.Conclusions: Our findings strongly supported that DCLK1-S isoform may play a crucial role in invasion, tumor aggressive behavior, and worsened DSS of the patients with CRC. Importantly, high cytoplasmic expression of DCLK1-S compared to moderate expression could be considered as an independent prognostic factor influencing DSS. Taken together, DCLK1-S can be a candidate as a promising prognostic and targeted-therapeutic indicator for effective treatment of CRC.
NK Cell Subpopulations and Receptor Expression in Recovering SARS-CoV-2 Infection
Authors: Saresella Marina , Trabattoni Daria , Marventano Ivana , Piancone Federica , Rosa Francesca La , Caronni Antonio , Lax Agata , Bianchi Luca , Bianchi Paolo , Navarro Jorge , Bolognesi Elisabetta , Zanzottera Milena , Guerini Franca Rosa , Clerici Mario
Published: March 2021

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the pandemic of coronavirus disease (COVID-19). Whereas in most cases COVID-19 is asymptomatic or pauci symptomatic, extremely severe clinical forms are observed. In this case complex immune dysregulations and an excessive inflammatory response are observed and are the main cause of morbidity and mortality. Natural Killer cells are key players in the control of viral infection and their activity is regulated by a tight balance between activating and inhibitory receptors; an alteration of NK activity was suggested to be associated with the development of severe forms of COVID-19. In this study we analyzed peripheral NK cell subpopulations and the expression of activating and inhibitory receptors in 30 in-patients suffering from chronic neurological conditions who recovered from mild, moderate or severe SARS-CoV-2 infection comparing the results to those of 10 SARS-CoV-2-uninfected patients. Results showed that an expansion of NK subset with lower cytolytic activity and an augmented expression of the 2DL1 inhibitory receptor, particularly when in association with the C2 ligand (KIR2DL1-C2), characterized the immunological scenario of severe COVID-19 infection; an increase of NK expressing the ILT2 inhibitory receptor was instead seen in patients recovering from mild or moderate infection compared to controls. Results herein suggest that the KIR2DL1-C2 NK inhibitory complex is a risk factor toward the development of severe form of COVID-19. Our results confirm that a complex alteration of NK activity is present in COVID-19 infection and offer a molecular explanation for this observation.
A Combined Spectroscopic and Theoretical Analysis of Plasmonic Silver Nanoparticles Sensor Towards Detailed Microscopic Understanding of Heavy Metal Detection
Authors: Pan Nivedita , Maji Tuhin Kumar , Banarjee Sayantika , Biswas Pritam , Chatterjee Arka , Mitra Mala , Chatterjee Arpita , Pal Samir Kumar Kumar
Published: March 2021

Plasmonic nanoparticles are of great importance owing to their highly responsive ‘Localized Surface Plasmon Resonance’ (LSPR) behaviour to self-agglomeration/ aggregation leading to the development of various nanosensors. Herein we demonstrated the definite self-assembly of citrate functionalized silver nanoparticles (AgNPs) into a one-dimensional linear chain in presence of charged lead ions (Pb 2+ ), one of the most toxic heavy metal pollutants. We have explored detail mechanism using a variety of spectroscopic tools and electron microscopy. The self-aggregation of AgNPs leads to the generation of new LSPR modes due to coupling of nearby existing modes. The conclusion of our experimental findings is duly supported by our developed numerical modelling based on the quasi-static approximation that the generated new LSPR modes are solely due to formation of chain-like aggregation of AgNPs. We have also monitored the LSPR spectra in presence of other metal ions, however, only Pb 2+ found to give such unique self-assembled geometry may due to its high interaction affinity with citrate. These findings play a key role for citrate functionalised AgNPs to be used as a low cost highly selective and sensitive lead ion sensor for potential application in industrial lead pollution monitoring. We have further varied several sensor parameters such as AgNPs size, concentration and the allowed reaction time for it to be practically implemented as an efficient lead sensor meeting the Environmental Protection Agency recommendations.