bluuebunny/crossref_metadata_2025 huggingface dataset:
Community Readiness for Drug Abuse Prevention in Two Rural Communities in Enugu State, Nigeria
Authors: Nwagu Evelyn N. , Dibia Samuel I. C. , Odo Amelia N.
Published: October 2020

Background

Drug abuse is an issue that affects all people regardless of gender, race, ethnicity, age, or socioeconomic status. It is associated with a range of physical, social, and economic consequences at both individual and community levels. Community involvement is necessary for effective drug abuse prevention. Communities need to be ready for such involvement.

Methods

We applied the Community Readiness Model and its assessment tool to determine the level of readiness for prevention of drug abuse in Obukpa and Ubollo communities of Nsukka and Udenu Local Government Areas of Enugu North Senatorial zone, Nigeria. Community Readiness Model and the Tool are very useful in identifying gaps in community readiness and capacity as well as guiding strategies for community initiatives. Twelve key informant interviews were conducted and analyzed according to procedures outlined in the Community Readiness handbook.

Results

The results of the study showed that members of Obukpa and Ubollo communities were at the vague awareness stage. SWOT analysis of the qualitative data revealed the strengths, weaknesses, opportunities and threats within the communities for an intended intervention to increase level of readiness. One such strength is the church, which was identified as a viable means of disseminating information on drug abuse prevention in both communities.

Conclusion

Rural communities are vulnerable to drug-related problems as a result of low levels of awareness of the dangers of drug abuse. The findings of this study provided strategies and directions for future intervention programmes in the study area. Nurses and other health personnel involved in Community Health Education should take cognizance of the strengths, weaknesses and opportunities within the communities in designing drug abuse prevention intervention programmes.
SARS-CoV-2–specific T cells are rapidly expanded for therapeutic use and target conserved regions of the membrane protein
Authors: Keller Michael D. , Harris Katherine M. , Jensen-Wachspress Mariah A. , Kankate Vaishnavi V. , Lang Haili , Lazarski Christopher A. , Durkee-Shock Jessica , Lee Ping-Hsien , Chaudhry Kajal , Webber Kathleen , Datar Anushree , Terpilowski Madeline , Reynolds Emily K. , Stevenson Eva M. , Val Stephanie , Shancer Zoe , Zhang Nan , Ulrey Robert , Ekanem Uduak , Stanojevic Maja , Geiger Ashley , Liang Hua , Hoq Fahmida , Abraham Allistair A. , Hanley Patrick J. , Cruz C. Russell , Ferrer Kathleen , Dropulic Lesia , Gangler Krista , Burbelo Peter D. , Jones R. Brad , Cohen Jeffrey I. , Bollard Catherine M.
Published: October 2020

T-cell responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been described in recovered patients, and may be important for immunity following infection and vaccination as well as for the development of an adoptive immunotherapy for the treatment of immunocompromised individuals. In this report, we demonstrate that SARS-CoV-2–specific T cells can be expanded from convalescent donors and recognize immunodominant viral epitopes in conserved regions of membrane, spike, and nucleocapsid. Following in vitro expansion using a good manufacturing practice-compliant methodology (designed to allow the rapid translation of this novel SARS-CoV-2 T-cell therapy to the clinic), membrane, spike, and nucleocapsid peptides elicited interferon-γ production, in 27 (59%), 12 (26%), and 10 (22%) convalescent donors (respectively), as well as in 2 of 15 unexposed controls. We identified multiple polyfunctional CD4-restricted T-cell epitopes within a highly conserved region of membrane protein, which induced polyfunctional T-cell responses, which may be critical for the development of effective vaccine and T-cell therapies. Hence, our study shows that SARS-CoV-2 directed T-cell immunotherapy targeting structural proteins, most importantly membrane protein, should be feasible for the prevention or early treatment of SARS-CoV-2 infection in immunocompromised patients with blood disorders or after bone marrow transplantation to achieve antiviral control while mitigating uncontrolled inflammation.
Correction to: Hepatocyte growth factor inhibits TGF-β1-induced myofibroblast differentiation in tendon fibroblasts: role of AMPK signaling pathway
Authors: Cui Qingbo , Fu Songbin , Li Zhaozhu
Published: October 2020

An amendment to this paper has been published and can be accessed via the original article.
2-D chemical structure image-based in silico model to predict agonist activity for androgen receptor
Authors: Yu Myeong-Sang , Lee Jingyu , Lee Yongmin , Na Dokyun
Published: October 2020

Background

Abnormal activation of human nuclear hormone receptors disrupts endocrine systems and thereby affects human health. There have been machine learning-based models to predict androgen receptor agonist activity. However, the models were constructed based on limited numerical features such as molecular descriptors and fingerprints.

Result

In this study, instead of the numerical features, 2-D chemical structure images of compounds were used to build an androgen receptor toxicity prediction model. The images may provide unknown features that were not represented by conventional numerical features. As a result, the new strategy resulted in a construction of highly accurate prediction model: Mathews correlation coefficient (MCC) of 0.688, positive predictive value (PPV) of 0.933, sensitivity of 0.519, specificity of 0.998, and overall accuracy of 0.981 in 10-fold cross-validation. Validation on a test dataset showed MCC of 0.370, sensitivity of 0.211, specificity of 0.991, PPV of 0.882, and overall accuracy of 0.801. Our chemical image-based prediction model outperforms conventional models based on numerical features.

Conclusion

Our constructed prediction model successfully classified molecular images into androgen receptor agonists or inactive compounds. The result indicates that 2-D molecular mimetic diagram would be used as another feature to construct molecular activity prediction models.
How does ageism influence frailty? A preliminary study using a structural equation model
Authors: Ye Bo , Gao Junling , Fu Hua , Chen Hao , Dong Wenjing , Gu Ming
Published: October 2020

Background

Based on the Stereotype Embodiment Theory (SET), this study aims to examine the mechanism of ageism on frailty through the proposed framework of “Experiences of Ageism (EA) → Age Stereotypes (AS) → Attitudes to Ageing (AA) → Frailty” using a structural equation model (SEM).

Methods

A community-based study involving 630 participants aged 60 years and older was conducted in Shanghai. EA, AS, AA and frailty status were assessed by validated scales. In particular, EA included three parts in this study, as the first part was the experiences of explicit prejudice or discrimination because of age, another two parts were the experiences of witnessed and encountered implicit negative age-based stereotypes. A SEM was performed to examine whether the proposed paths from EA to frailty were supported.

Results

EA had a significant indirect effect (β’ = .360*-.456*-.576 = .095,p < .001) on frailty through the path of “EA → AS → AA → Frailty” after controlling for covariates. AA had a direct effect (β = −.576,p < .001) on frailty; AS fully mediated the association between EA and AA (indirect effect = .360*-.456 = −.164,p < .001), and AA fully mediated the association between AS and frailty (indirect effect = −.456*-.576 = .263,p < .001).

Conclusions

These findings demonstrated a mechanism from ageism to frailty, and highlighted the potential threat of negative AS on health. Ageism and frailty are both great challenges for the process of healthy ageing.
Genomic evidence for recurrent genetic admixture during the domestication of Mediterranean olive trees (Olea europaea L.)
Authors: Julca Irene , Marcet-Houben Marina , Cruz Fernando , Gómez-Garrido Jèssica , Gaut Brandon S. , Díez Concepción M. , Gut Ivo G. , Alioto Tyler S. , Vargas Pablo , Gabaldón Toni
Published: October 2020

Background

Olive tree (Olea europaea L. subsp. europaea, Oleaceae) has been the most emblematic perennial crop for Mediterranean countries since its domestication around 6000 years ago in the Levant. Two taxonomic varieties are currently recognized: cultivated (var. europaea) and wild (var. sylvestris) trees. However, it remains unclear whether olive cultivars derive from a single initial domestication event followed by secondary diversification, or whether cultivated lineages are the result of more than a single, independent primary domestication event. To shed light into the recent evolution and domestication of the olive tree, here we analyze a group of newly sequenced and available genomes using a phylogenomics and population genomics framework.

Results

We improved the assembly and annotation of the reference genome, newly sequenced the genomes of twelve individuals: ten var. europaea, one var. sylvestris, and one outgroup taxon (subsp. cuspidata)—and assembled a dataset comprising whole genome data from 46 var. europaea and 10 var. sylvestris. Phylogenomic and population structure analyses support a continuous process of olive tree domestication, involving a major domestication event, followed by recurrent independent genetic admixture events with wild populations across the Mediterranean Basin. Cultivated olives exhibit only slightly lower levels of genetic diversity than wild forms, which can be partially explained by the occurrence of a mild population bottleneck 3000–14,000 years ago during the primary domestication period, followed by recurrent introgression from wild populations. Genes associated with stress response and developmental processes were positively selected in cultivars, but we did not find evidence that genes involved in fruit size or oil content were under positive selection. This suggests that complex selective processes other than directional selection of a few genes are in place.

Conclusions

Altogether, our results suggest that a primary domestication area in the eastern Mediterranean basin was followed by numerous secondary events across most countries of southern Europe and northern Africa, often involving genetic admixture with genetically rich wild populations, particularly from the western Mediterranean Basin.
Communities on the move: community participation in health in rural territories of Buenaventura District in Colombia
Authors: Blandón-Lotero Laura Catalina , Jaramillo-Mejía Marta Cecilia
Published: October 2020

Background

Social and community participation is a fundamental component of the development of renewed primary healthcare (PHC). With the recognition of health as a right, such participation is a significant part of the design of public policies aimed at this sector. These policies contribute not only to overcoming inequity in the provision of this type of services but also to a reduction in social inequalities as a whole. Through a comparative analysis, this study aimed to explain the conditions through which ethnic-rural territories of the Colombian Pacific coast participate in health to contribute to the generation of policies and programs in territories with similar conditions.

Methods

The work was developed through the use of multiple techniques and strategies for information collection and analysis. These include several semi-structured interviews, multiple observation exercises and analysis based on a set theory, i.e., qualitative comparative analysis (QCA). The latter aims to develop a model that provides a count of the main causal combinations that allow high community participation in health.

Results

Key findings include how the trajectory of social mobilization and existence of a robust community social fabric became two critical conditions for community participation in the context of social exclusion. The presence of variables such as the implementation of PHC, guarantee of social rights, and trust in institutions, is underestimated as sufficient causal conditions for obtaining this result. Therefore, it is essential to recognize the existence, validity, and importance of processes, experiences, and resourcefulness of political natures, which aim at transforming the daily reality of the inhabitants of these communities. These also set a potential space and scenario for managing the communities’ main problems, including health, in the absence of institutionality that guarantees access to their social rights.

Conclusion

This study points out the importance of understanding community participation as a political activity, expanding exchange dynamics and dialogs between institutions, rulers, and communities to provide social responses in health and well-being to communities and to understand local realities and their own community dynamics.
Inequality in healthcare use among older people in Colombia
Authors: Garcia-Ramirez Jorge , Nikoloski Zlatko , Mossialos Elias
Published: October 2020

Background

Since the early 1990s, Colombia has made great strides in extending healthcare coverage to its population. In order to measure the impact of these efforts, it is important to assess whether the introduction of universal health coverage has translated into equitable access to healthcare in the country, particularly for the elderly. Thus, in this study we assessed the inequality in utilization of health services among elderly patients in Colombia. In addition, we identified the determinants of healthcare utilization.

Methods

We analyzed the 2015 Colombian health, well-being and aging study (SABE). To classify determinants of healthcare use into predisposing, enabling and need factors, we employed the Anderson framework of healthcare utilization. Use of outpatient, inpatient and preventive health services constituted the dependent variables. We performed multivariate logistic regressions, estimated concentration indexes (CI) and performed decomposition analyses of the CIs to determine the contribution of various determinants to inequality of healthcare utilization.

Results

The study sample included 23,694 adults over 60-years-old. Wealth quintile, urban dwelling, health insurance type and multimorbidity predicted the utilization of all types of healthcare services except for hospitalization. Aside from inpatient care, pro-rich inequality in utilization of healthcare services was present. Wealth quintile and type of health insurance were the largest contributors to pro-rich inequality in use of preventive services.

Conclusions

While there has been progress in health insurance coverage for the elderly in Colombia, there are still equality challenges in the delivery of healthcare, especially for preventive and outpatient care. These inequalities are driven by individual characteristics such as wealth, urban residence, type of health insurance carried, and presence of multimorbidity. To address this issue, the Colombian health system should extend health insurance coverage to uninsured populations, as well as reduce barriers of access to healthcare services among poorest and the rural population receiving subsidized insurance.
Ketogenic diet aggravates cardiac remodeling in adult spontaneously hypertensive rats
Authors: You Yuehua , Guo Yongzheng , Jia Ping , Zhuang Biaobiao , Cheng Yu , Deng Hongpei , Wang Xiaowen , Zhang Cheng , Luo Suxin , Huang Bi
Published: October 2020

Background

Ketogenic diet (KD) has been proposed to be an effective lifestyle intervention in metabolic syndrome. However, the effects of KD on cardiac remodeling have not been investigated. Our aim was to investigate the effects and the underling mechanisms of KD on cardiac remodeling in spontaneously hypertensive rats (SHRs).

Methods

10-week-old spontaneously hypertensive rats were subjected to normal diet or ketogenic diet for 4 weeks. Then, their blood pressure and cardiac remodeling were assessed. Cardiac fibroblasts were isolated from 1- to 3-day-old neonatal pups. The cells were then cultured with ketone body with or without TGF-β to investigate the mechanism in vitro.

Results

4 weeks of KD feeding aggravated interstitial fibrosis and cardiac remodeling in SHRs. More interestingly, ketogenic diet feeding increased the activity of mammalian target of rapamyoin (mTOR) complex 2 pathway in the heart of SHRs. In addition, β-hydroxybutyrate strengthened the progression of TGF-β-induced fibrosis in isolated cardiac fibroblasts. mTOR inhibition reversed this effect, indicating that ketone body contributes to cardiac fibroblasts via mTOR pathway.

Conclusions

These data suggest that ketogenic diet may lead to adverse effects on the remodeling in the hypertensive heart, and they underscore the necessity to fully evaluate its reliability before clinical use.
Ten important roles for academic leaders in data science
Authors: Moore Jason H.
Published: October 2020

Data science has emerged as an important discipline in the era of big data and biological and biomedical data mining. As such, we have seen a rapid increase in the number of data science departments, research centers, and schools. We review here ten important leadership roles for a successful academic data science chair, director, or dean. These roles include the visionary, executive, cheerleader, manager, enforcer, subordinate, educator, entrepreneur, mentor, and communicator. Examples specific to leadership in data science are given for each role.
RNA editing in cancer impacts mRNA abundance in immune response pathways
Authors: Chan Tracey W. , Fu Ting , Bahn Jae Hoon , Jun Hyun-Ik , Lee Jae-Hyung , Quinones-Valdez Giovanni , Cheng Chonghui , Xiao Xinshu
Published: October 2020

Background

RNA editing generates modifications to the RNA sequences, thereby increasing protein diversity and shaping various layers of gene regulation. Recent studies have revealed global shifts in editing levels across many cancer types, as well as a few specific mechanisms implicating individual sites in tumorigenesis or metastasis. However, most tumor-associated sites, predominantly in noncoding regions, have unknown functional relevance.

Results

Here, we carry out integrative analysis of RNA editing profiles between epithelial and mesenchymal tumors, since epithelial-mesenchymal transition is a key paradigm for metastasis. We identify distinct editing patterns between epithelial and mesenchymal tumors in seven cancer types using TCGA data, an observation further supported by single-cell RNA sequencing data and ADAR perturbation experiments in cell culture. Through computational analyses and experimental validations, we show that differential editing sites between epithelial and mesenchymal phenotypes function by regulating mRNA abundance of their respective genes. Our analysis of RNA-binding proteins reveals ILF3 as a potential regulator of this process, supported by experimental validations. Consistent with the known roles of ILF3 in immune response, epithelial-mesenchymal differential editing sites are enriched in genes involved in immune and viral processes. The strongest target of editing-dependent ILF3 regulation is the transcript encoding PKR, a crucial player in immune and viral response.

Conclusions

Our study reports widespread differences in RNA editing between epithelial and mesenchymal tumors and a novel mechanism of editing-dependent regulation of mRNA abundance. It reveals the broad impact of RNA editing in cancer and its relevance to cancer-related immune pathways.
PRECIOUS: PREvention of Complications to Improve OUtcome in elderly patients with acute Stroke—statistical analysis plan of a randomised, open, phase III, clinical trial with blinded outcome assessment
Authors: de Jonge Jeroen C. , Woodhouse Lisa J. , Reinink Hendrik , van der Worp H. Bart , Bath Philip M. ,   for the PRECIOUS investigators
Published: October 2020

Rationale

Aspiration, infections, and fever are common in the first days after stroke, especially in older patients. The occurrence of these complications has been associated with an increased risk of death or dependency.

Aims and design

PREvention of Complications to Improve OUtcome in elderly patients with acute Stroke (PRECIOUS) is an international, multi-centre, 3 × 2 factorial, randomised, controlled, open-label clinical trial with blinded outcome assessment, which will assess whether prevention of aspiration, infections, or fever with metoclopramide, ceftriaxone, paracetamol, respectively, or any combination of these in the first 4 days after stroke onset improves functional outcome at 90 days in elderly patients with acute stroke.

Discussion

This statistical analysis plan provides a technical description of the statistical methodology and unpopulated tables and figures. The paper is written prior to data lock and unblinding of treatment allocation.

Trial registration

ISRCTN registry ISRCTN82217627. Registered on 22 September 2015. The trial was prospectively registered.
NOn-invasive Vagus nerve stimulation in acute Ischemic Stroke (NOVIS): a study protocol for a randomized clinical trial
Authors: van der Meij Anne , van Walderveen Marianne A. A. , Kruyt Nyika D. , van Zwet Erik W. , Liebler Eric J. , Ferrari Michel D. , Wermer Marieke J. H.
Published: October 2020

Background

Secondary damage due to neurochemical and inflammatory changes in the penumbra in the first days after ischemic stroke contributes substantially to poor clinical outcome. In animal models, vagus nerve stimulation (VNS) inhibits these detrimental changes and thereby reduces tissue injury. The aim of this study is to investigate whether non-invasive cervical VNS (nVNS) in addition to the current standard treatment can improve penumbral recovery and limit final infarct volume.

Methods

NOVIS is a single-center prospective randomized clinical trial with blinded outcome assessment. One hundred fifty patients will be randomly allocated (1:1) within 12 h from clinical stroke onset to nVNS for 5 days in addition to standard treatment versus standard treatment alone. The primary endpoint is the final infarct volume on day 5 assessed with MRI.

Discussion

We hypothesize that nVNS will result in smaller final infarct volumes as compared to standard treatment due to improved penumbral recovery. The results of this study will be used to assess the viability and approach to power a larger trial to more definitively assess the clinical efficacy of nVNS after stroke.

Trial registration

ClinicalTrials.govNCT04050501. Registered on 8 August 2019
Assisted life termination and truth telling to terminally ill patients – a cross-sectional study of public opinions in Israel
Authors: Bodas Moran , Velan Baruch , Kaplan Giora , Ziv Arnona , Rubin Carmit , Peleg Kobi
Published: October 2020

Background

End-of-life decisions are highly complex socio-normative and ethical phenomena. The goal of this study was to provide an assessment of public opinions in Israel concerning aspects of end-of-life decisions.

Methods

An online cross sectional study was performed in February 2020. The primary tool including items pertaining to death assistance and truth telling to patients. A sample of 515 participants representative of the adult Israeli population was obtained.

Results

The majority of participants (71%) supports telling the entire truth to patients even in harsh conditions. Support for truth telling decreases with affiliation to religion, with as little as 40% support among ultra-orthodox. People with vocational education are the least supportive of truth telling. Concerning doctor assisted death, almost half (49%) of the sample were supportive. Opposition is positively associated with religiosity, with 90% of ultra-orthodox and 58% of religious participants opposing doctor-assisted death, compared to only 18% among seculars. Non-Jews were 3.35 times (95%CI: 1.90, 5.91) more likely to oppose doctor assisted death than Jews (p < .0001). An Interrelationship analysis crossing between attitudes revealed that the largest group (39%) was comprised of participants who support both (“autonomists”).

Conclusions

Israelis are overwhelmingly supportive of truth telling to patients. In contrast, Israeli public opinions on doctor assisted death are divided. For both attitudes, religiousness plays a crucial role as a catalyst for conservatism and opposition to change. Almost a half of the public is also supportive of an autonomist approach that would allow patients to decide on ending their own lives.
A review of jellyfish aggregations, focusing on India’s coastal waters
Authors: Baliarsingh Sanjiba Kumar , Lotliker Aneesh Anandrao , Srichandan Suchismita , Samanta Alakes , Kumar Nimit , Nair T. M. Balakrishnan
Published: October 2020

A review of jellyfish aggregations focused on India’s coastal waters was conducted, with the aim to enhance understanding of conducive conditions and subsequent ecological impacts. Jellyfish swarming, as well as their beach strandings, have been reported from many areas of the world—including India’s coastal waters. A variety of natural (winds, tidal fronts, surface currents, water temperature, salinity, turbidity, dissolved oxygen) and anthropogenic (water quality deterioration, overfishing, translocation, habitat modification) factors play pivotal roles in triggering jellyfish aggregations. Jellyfish aggregation events in the forms of their swarming in coastal waters and beach strandings have resulted in ephemeral nuisances such as water quality deterioration, food chain alterations, hindrance in seawater uptake by power plants, clogging of nets during fishing operations, and tourism declines. Several well-known Indian tourist beaches (e.g., Puri, Chennai, Goa, and Mumbai) have experienced beach strandings. Despite recurrence of such events, jellyfishes are relatively less scientifically investigated and monitored in Indian coastal waters. Therefore, it is important to determine the environmental conditions that trigger jellyfish swarming, in order to develop effective monitoring and prediction strategies. This study additionally proposes a conceptual framework towards development of a jellyfish monitoring system for Indian waters using satellite and model data.
Perceived psychosocial health and its sociodemographic correlates in times of the COVID-19 pandemic: a community-based online study in China
Authors: Wang Gan-Yi , Tang Shang-Feng
Published: October 2020

Background

Coronavirus disease 2019 (COVID-19) pandemic has been affecting people's psychosocial health and well-being through various complex pathways. The present study aims to investigate the perceived psychosocial health and its sociodemographic correlates among Chinese community-dwelling residents.

Methods

This cross-sectional survey was carried out online and using a structured questionnaire during April 2020. In total, 4788 men and women with the age range of 11–98 years from eight provinces in eastern, central and western China were included in the analysis. We adopted a tactical approach to capture three key domains of perceived psychosocial health that are more likely to occur during a pandemic including hopelessness, loneliness, and depression. Multiple regression method, binary logistic regression model and variance inflation factor (VIF) were used to conduct data analysis.

Results

Respectively 34.8%, 32.5% and 44.8% of the participants expressed feeling more hopeless, lonely, and depressed during the pandemic. The percentage of all three indicators was comparatively higher among women than among men: hopelessness (50.7% vs 49.3%), loneliness (52.4% vs 47.6%), and depression (56.2% vs 43.8%). Being married was associated with lower odds of loneliness among men (odds ratio [OR] = 0.63, 95% CI: 0.45–0.90). Loneliness was negatively associated with smoking (OR = 0.67, 95% CI: 0.45–0.99) and positively associated with drinking (OR = 1.45, 95% CI: 1.04–2.02). Compared with those in the lowest income bracket (< CNY 10 000), men (OR = 0.34, 95% CI: 0.21–0.55) and women (OR = 0.36, 95% CI: 0.23–0.56) in the highest level of annually housed income (> CNY 40 000) had the lowest odds of reporting perceived hopelessness (OR = 0.35, 95% CI: 0.25–0.48). Smoking also showed negative association with depression only among men (OR = 0.63, 95% CI: 0.43–0.91).

Conclusions

More than one-third of the participants reported worsening in the experience of hopelessness and loneliness, with more than two-fifth of worsening depression during the pandemic compared with before the outbreak. Several socioeconomic and lifestyle factors were found to be associated with the outcome variables, most notably participants' marital status, household income, smoking, alcohol drinking, existing chronic conditions. These findings may be of significance to treat patients and help them recover from the pandemic.
Early year undergraduate researchers’ reflections on the values and perceived costs of their research experience
Authors: Ceyhan Gaye D. , Tillotson John W.
Published: October 2020

Background

Prior research reported that motivational beliefs that individuals attach to specific tasks predict continuing interest and persistence in the task. A motivational approach may be particularly useful for understanding undergraduate students’ engagement with research in their first and second years in college. The current study utilizes the expectancy-value theory of achievement motivation to qualitatively explore how much and in what ways early year undergraduate researchers value their research experience and what kinds of costs they associate with it.

Results

The results revealed that intrinsic value had the highest expression in participants’ motivation to engage in research. The second most expressed value type was the utility value of undergraduate research with regards to obtaining the desired outcomes, and attainment value played the least important role in participants’ motivation to engage in research. Findings also indicated that some of the participants associated a cost(s) to their research experience. The highest mentioned perceived cost was opportunity cost, where participants commented on losing other valued alternatives when engaging in research. Participants commented on the time, effort, or amount of work needed to engage in research, and a few participants commented on the emotional cost associated with their research experience in terms of the fear of failure.

Conclusion

As perceived cost is the least studied in the expectancy-value framework, this study contributes to cost values within college students, particularly about early year undergraduate researchers. The findings of this study can form the basis for future work on exploring ways to increase the values and decrease the costs students experience in their undergraduate research experiences.
Development and fabrication of disease resistance protein in recombinant Escherichia coli
Authors: Effendi Sefli Sri Wahyu , Tan Shih-I , Chang Chien-Hsiang , Chen Chun-Yen , Chang Jo-Shu , Ng I-Son
Published: October 2020

Cyanobacteria and Spirulina produce C-phycocyanin (CPC), a water soluble protein associated pigment, which is extensively used in food and pharmaceutical industries. Other therapeutic proteins might exist in microalgal cells, of which there is limited knowledge. Such proteins/peptides with antibiotic properties are crucial due to the emergence of multi-drug resistant pathogens. In addition, the native expression levels of such disease resistant proteins are low, hindering further investigation. Thus, screening and overexpression of such novel proteins is urgent and important. In this study, a protein which was identified as a putative disease resistance protein (DRP) in the mixture of Spirulina product has been explored for the first time. To improve protein expression, DRP was cloned in the pET system, co-transformed with pRARE plasmid for codon optimization and was significantly overexpressed in E. coli BL21(DE3) under induction with isopropyl-β-d-1-thiogalactopyranoside (IPTG). Furthermore, soluble DRP exhibited intense antimicrobial activity against predominant pathogens, and an inhibition zone of 1.59 to 1.74 cm was obtained for E. coli. At a concentration 4 mg/mL, DRP significantly elevated the growth of L. rhamnosus ZY up to twofold showing probable prebiotic activities. Moreover, DRP showed potential as an effective antioxidant, and the scavenging ability for ROS was in the order of hydroxyl > DPPH > superoxide radicals. A putative disease resistance protein (DRP) has been identified, sequenced, cloned and over-expressed in E. coli as a functional protein. Thus expressed DRP showed potential anti-microbial and antioxidant properties, with promising therapeutic applications.
Wait, It’s a Discourse Marker
Authors: Tagliamonte Sali A.
Published: October 2020

This study investigates a discourse-pragmatic use of the word wait in spoken North American English. This function is an extension from an original lexical meaning of pausing or lingering, which has expanded to indicate a pause in discourse as a speaker reflects on or corrects an earlier topic. Over 340 examples from 211 individuals permit comparative sociolinguistic methods and statistical modeling to offer an early assessment of the variation among alternates of this innovative use and to test for broad social and linguistic factors in order to understand the underlying processes. The results expose notable recent developments: older people use the longer, more temporally specified variants wait a minute and wait a second, while wait alone is increasing in apparent time, with women leading its advance. The robust increase in the use of wait alone (e.g., I haven’t seen her yet. No wait. Yes, I have), co-occurrence with other markers (e.g., no), and the function of self-correction/commentary arises after 1970. The unique contribution of socially stratified corpora also demonstrates that this development follows well-known principles of linguistic change, as wait develops from a verb with temporal specification to a full-fledged discourse-pragmatic marker on the left periphery.
Introduction: Knowledge of Birds and Feathers in the Ancient and Colonial Mesoamerican World
Authors: Sousa Lisa , Caplan Allison
Published: October 2020

Birds and their feathers have long occupied a unique place in the social, cultural, and intellectual life of the Americas. This was particularly so in Mesoamerica, where ancient civilizations and colonial societies developed extensive knowledge of birds, their behaviors and habitats, and their vibrant plumage. This special issue brings together scholars from a variety of disciplines, including art history, history, and biology, to promote discussion among the arts, social sciences, and natural sciences on the role of birds and feathers in Mesoamerica. This introductory essay first provides a discussion of the major trends in the scholarship on birds and feathers in ancient and colonial Mesoamerica. It then highlights the contributions of the articles in the special issue to our understanding of the multifaceted roles that both symbolic and real birds and their feathers played in indigenous and transatlantic knowledge systems and societies.
Bridging Biology and Ethnohistory: A Case for Collaboration
Authors: Caplan Allison , Maley James M. , McCormack John E.
Published: October 2020

Interdisciplinary scholarship that combines research questions and methodologies from biology and ethnohistory generates new insights into historical interactions between human and bird populations in ancient and colonial Mesoamerica. Codices, ethnohistorical sources, and surviving feather art point to the religious, economic, and artistic importance of various types of birds to Nahua people. Alongside the well-known resplendent quetzal and lovely cotinga, many additional species were significant to ancient and colonial Nahuas. This article presents potential directions for scholarship that bridge biology and ethnohistory and surveys key resources, including natural history collections and online databases. Finally, the article employs the biological literature to describe eleven bird species of great importance to Nahuas, detailing the species’ appearance and plumage, geographic range, variation, habitat, behaviors, and current status. Ultimately, the article demonstrates how insights from natural history and ethnohistory together allow for a fuller understanding of Nahuas’ material and conceptual interactions with these birds.
The Tecolotl and the Chiquatli: Omens of Death and Transspecies Dialogues in the Aztec World
Authors: García Garagarza León
Published: October 2020

This essay examines some instances of interspecific dialogues between owls and human beings recorded in Nahuatl-language sources from the sixteenth century. Since ancient times, owls have been considered omens of death in Mexico. This article analyzes the cultural and linguistic context of this belief among the contact-period Nahuas: the import of tetzahuitl (omens) in the animistic worldview of the Aztecs, as well as the characteristic semantic pair in tecolotl, in chiquatli (“the owl, the barn owl”) to signify the lethal activities of the most representative messengers of the Lords of Death and Destiny, Mictlantecuhtli and Tezcatlipoca. Moreover, the essay shows how the ancient Nahuas considered the intelligibility of animal languages and engaged in active dialogues with the animal representatives of the gods, a form of communication that encompassed both the private and public spheres, as in these dialogues matters of disease, pollution, and warfare came into consideration.
Swift, Secret History, and War
Authors: Rabb Melinda Alliker
Published: October 2020

“Swift, Secret History, and War” argues that the relationship between Swift’s writing, reading, and his abiding interest in the English Civil Wars produced a distinctive contribution to the discourses that arose after the reestablishment of monarchy, called “secret histories.” These narratives claim to expose clandestine acts, to pull away veils that hide petty motives, and to expose abuses underlying the exercise of power. In Swift’s work, however, the impulse to dig up embarrassing or disillusioning secrets serves yet another purpose; it allows more painful realities to remain buried and thus provides a means of displacing, postponing, and avoiding direct confrontation with the devastation caused by war. The following discussion identifies and analyzes some of the ways in which traumatic conflict—especially within a nation in which neighbor has fought neighbor—requires indirection, delayed response, and the transference of the burden of representation onto succeeding generations. Literature can play a crucial role in the process of displacement when human history has proven (as Gulliver’s Houyhnhnm master observes) “capable of such Enormities, . . . worse than Brutality itself.” Swift’s distinctive deployment of secret history’s strategies allows an ironic historian a mechanism to look directly at and disclose some of the follies and vices of his culture, but also to remain cognizant, at some level, of what remains buried under “the heap” of a traumatic past.
From head to tail: regionalization of the neural crest
Authors: Rocha Manuel , Beiriger Anastasia , Kushkowski Elaine E. , Miyashita Tetsuto , Singh Noor , Venkataraman Vishruth , Prince Victoria E.
Published: October 2020

The neural crest is regionalized along the anteroposterior axis, as demonstrated by foundational lineage-tracing experiments that showed the restricted developmental potential of neural crest cells originating in the head. Here, we explore how recent studies of experimental embryology, genetic circuits and stem cell differentiation have shaped our understanding of the mechanisms that establish axial-specific populations of neural crest cells. Additionally, we evaluate how comparative, anatomical and genomic approaches have informed our current understanding of the evolution of the neural crest and its contribution to the vertebrate body.
A low-cost method for carrying loads during human walking
Authors: Arellano Christopher J. , McReynolds Obioma B. , Thomas Shernice A.
Published: October 2020

Humans often perform tasks that require them to carry loads, but the metabolic cost of carrying loads depends on where the loads are positioned on the body. We reasoned that carrying loads at the arms’ center of mass (COM) during walking might be cheap since arm swing is thought to be dominated by passive pendulum dynamics. In contrast, we expected that carrying loads at the leg's COM would be relatively expensive because muscular actuation is necessary to initiate and propagate leg swing. Therefore, we hypothesized that carrying loads at the arm's COM while swinging would be cheaper than carrying loads at the leg's COM. We further hypothesized that carrying loads at the arm's COM while swinging would be more expensive than carrying loads at the waist, where the mass does not swing relative to the body. We measured net metabolic power, arm and leg motion, and the free vertical moment while subjects (n=12) walked on a treadmill (1.25 m/s) with no-load, and with 8-kg added to the arms (swinging vs. not swinging), legs, or waist. We found that carrying loads on the arms or legs altered arm swinging amplitude; however, the free vertical moment remained similar across conditions. Most notably, the cost of carrying loads on the swinging arms was 9% less than carrying at the leg's COM (P<0.001), but similar to the waist (P=0.529). Overall, we find that carrying loads at the arm's COM is just as cheap as carrying loads at the waist.
Prolonged exposure to stressors suppresses exploratory behavior in zebrafish larvae
Authors: Haney William A. , Moussaoui Bushra , Strother James A.
Published: October 2020

Environmental stressors induce rapid physiological and behavioral shifts in vertebrate animals. However, the neurobiological mechanisms responsible for stress-induced changes in behavior are complex and not well understood. Similar to mammalian vertebrates, zebrafish adults display a preference for dark environments that is associated with predator avoidance, enhanced by stressors, and broadly used in assays for anxiety-like behavior. Although the larvae of zebrafish are a prominent model organism for understanding neural circuits, fewer studies have examined the effects of stressors on their behavior. This study examines the effects of noxious chemical and electric shock stressors on locomotion and light preference in zebrafish larvae. We found that both stressors elicited similar changes in behavior. Acute exposure induced increased swimming activity, while prolonged exposure depressed activity. Neither stressor produced a consistent shift in light/dark preference, but prolonged exposure to these stressors resulted in a pronounced decrease in exploration of different visual environments. We also examined the effects of exposure to a noxious chemical cue using whole-brain calcium imaging, and identified neural correlates in the area postrema, an area of the hindbrain containing noradrenergic and dopaminergic neurons. Pharmaceutical blockade experiments showed that ɑ-adrenergic receptors contribute to the behavioral response to an acute stressor but are not necessary for the response to a prolonged stressor. These results indicate that zebrafish larvae have complex behavioral responses to stressors comparable to those of adult animals, and also suggest that these responses are mediated by similar neural pathways.
Sensitivity of RT-PCR testing of upper respiratory tract samples for SARS-CoV-2 in hospitalised patients: a retrospective cohort study
Authors: Williams Thomas C. , Wastnedge Elizabeth , McAllister Gina , Bhatia Ramya , Cuschieri Kate , Kefala Kallirroi , Hamilton Fiona , Johannessen Ingólfur , Laurenson Ian F. , Shepherd Jill , Stewart Alistair , Waters Donald , Wise Helen , Templeton Kate E.
Published: October 2020

Background: This study aimed to determine the sensitivity and specificity of reverse transcription PCR (RT-PCR) testing of upper respiratory tract (URT) samples from hospitalised patients with coronavirus disease 2019 (COVID-19), compared to the gold standard of a clinical diagnosis.

Methods: All URT RT-PCR testing for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in NHS Lothian, Scotland, United Kingdom between the 7th of February and 19th April 2020 (inclusive) was reviewed, and hospitalised patients were identified. All URT RT-PCR tests were analysed for each patient to determine the sequence of negative and positive results. For those who were tested twice or more but never received a positive result, case records were reviewed, and a clinical diagnosis of COVID-19 allocated based on clinical features, discharge diagnosis, and radiology and haematology results. For those who had a negative RT-PCR test but a clinical diagnosis of COVID-19, respiratory samples were retested using a multiplex respiratory panel, a second SARS-CoV-2 RT-PCR assay, and a human RNase P control.

Results: Compared to the gold standard of a clinical diagnosis of COVID-19, the sensitivity of a single upper respiratory tract RT-PCR for COVID-19 was 82.2% (95% confidence interval 79.0-85.1%).   The sensitivity of two upper respiratory tract RT-PCR tests increased sensitivity to 90.6% (CI 88.0-92.7%). A further 2.2% and 0.9% of patients who received a clinical diagnosis of COVID-19 were positive on a third and fourth test; this may be an underestimate of the value of further testing as the majority of patients 93.0% (2999/3226) only had one or two URT RT-PCR tests.

Conclusions: The sensitivity of a single RT-PCR test of URT samples in hospitalised patients is 82.2%. Sensitivity increases to 90.6% when patients are tested twice.  A proportion of cases with clinically defined COVID-19 never test positive on URT RT-PCR despite repeat testing.
Performance of baited traps for integrated management of Hypothenemus hampei Ferrari (Coleoptera: Scolytinae) in a conilon coffee crop in Rondônia State, Brazil
Authors: De Souza Moisés Santos , Costa José Nilton Medeiros , Espindula Marcelo Curitiba , Silva Alexandre de Almeida e
Published: October 2020

Hypothenemus hampei (Ferrari) is an important pest worldwide. Methods of monitoring and control using baited traps are not yet established in coffee plantations in the Brazilian Amazon. The objective of this work was to record, for the first time, results of the use of baited traps in coffee plantation located in Rondônia, in favor of the control and pest monitoring. Two areas were delineated: i) with use of the traps baited with  ethanol/methanol (1:1), treatment; ii) without use of traps (control). For comparison of results, two factors were considered: damaged fruits (damage by H. hampei) and infested (H. hampei inside of fruits). It was observed higher levels of damaged fruits per plants in the control area compared to the area where traps were used. The density of the pest population per plants found on infested fruits was also higher in the control area compared to the trapping area. These results suggest that traps baited with ethanol/methanol (1:1) are an effective alternative for population control of pest also in the coffee plantations in Rondônia, where there is no such management with this tool. Use of the baited traps to monitor the insect accurately revealed that the flight stimulus of the colonizing females is influenced by values of the environmental variables. According to the results, colonizing females are more active in the afternoon. Therefore, in order to achieve more efficient control of H. hampei, the best time to apply control agents is between 2:00 pm and 6:00 pm.