bluuebunny/crossref_metadata_2025 huggingface dataset:
Improving Prognosis of Surrogate Assay for Breast Cancer Patients by Absolute Quantitation of Ki67 Protein Levels Using Quantitative Dot Blot (QDB) Method
Authors: Hao Junmei , Lyu Yan , Zou Jiarui , Zhang Yunyun , Xie Shuishan , Jing Lili , Tang Fangrong , Lyu Jiahong , Zhang Wenfeng , Zhang Jianbo , Wang Xunting , Chen Kuisheng , Zhang Jiandi
Published: September 2021

Background

Immunohistochemistry (IHC)-based surrogate assay is the prevailing method in daily clinical practice to determine the necessity of chemotherapy for Luminal-like breast cancer patients worldwide. It relies on Ki67 scores to separate Luminal A-like from Luminal B-like breast cancer subtypes. Yet, IHC-based Ki67 assessment is known to be plagued with subjectivity and inconsistency to undermine the performance of the surrogate assay. A novel method needs to be explored to improve the clinical utility of Ki67 in daily clinical practice.

Materials and Methods

The Ki67 protein levels in a cohort of 253 specimens were assessed with IHC and quantitative dot blot (QDB) methods, respectively, and used to assign these specimens into Luminal A-like and Luminal B-like subtypes accordingly. Their performances were compared with the Kaplan–Meier, univariate, and multivariate survival analyses of the overall survival (OS) of Luminal-like patients.

Results

The surrogate assay based on absolutely quantitated Ki67 levels (cutoff at 2.31 nmol/g) subtyped the Luminal-like patients more effectively than that based on Ki67 scores (cutoff at 14%) (Log rank test, p = 0.00052 vs. p = 0.031). It is also correlated better with OS in multivariate survival analysis [hazard ratio (HR) at 6.89 (95% CI: 2.66–17.84, p = 0.0001) vs. 2.14 (95% CI: 0.89–5.11, p = 0.087)].

Conclusions

Our study showed that the performance of the surrogate assay may be improved significantly by measuring Ki67 levels absolutely, quantitatively, and objectively using the QDB method.
MiR-1224 Acts as a Prognostic Biomarker and Inhibits the Progression of Gastric Cancer by Targeting SATB1
Authors: Han Guo-Dong , Sun Yuan , Hui Hong-Xia , Tao Ming-Yue , Liu Yang-Qing , Zhu Jing
Published: September 2021

Objective

MiR-1224 has been reported to exhibit abnormal expression in several tumors. However, the expressing pattern and roles of miR-1224 in gastric cancer (GC) remain unclear. Our current research aimed to explore the potential involvement of miR-1224 in the GC progression.

Materials and Methods

The expression of miR-1224 was examined in tissue samples of 128 GC patients and cell lines by RT-PCR. Besides, the associations of miR-1224 expressions with clinicopathologic features and prognosis of GC patients were analyzed. Then, the possible influences of miR-1224 on cell proliferation and cell migration were determined. Afterward, the molecular target of miR-1224 was identified using bioinformatics assays and confirmed experimentally. Finally, RT-PCR and Western blot assays were performed to investigate the effect of the abnormal miR-1224 expression on the EMT and Wnt/β-catenin pathway.

Results

miR-1224 was lowly expressed in the GC specimens and cell lines due to T classification and TNM stage. Survival assays demonstrated that GC patients with low expressions of miR-1224 possessed poor overall survivals. Moreover, in vitro and in vivo assays revealed that the overexpression of miR-1224 inhibited cell proliferation, migration, and invasion in GC cells. SATB homeobox 1 (SATB1) was verified as a direct target of miR-1224 in GC. Furthermore, β-catenin and c-myc were significantly inhibited in miR-1224-overexpression cells.

Conclusions

Our findings highlight the potential of miR-1224 as a therapeutic target and novel biomarker for GC patients
Pediatric Telehealth Expansion in Response to COVID-19
Authors: Williams Stormee , Hill Kristina , Xie Luyu , Mathew M. Sunil , Ofori Ashley , Perry Tamara , Wesley Danielle , Messiah Sarah E.
Published: September 2021

Introduction: Telehealth utilization has been steadily increasing for the past two decades and has been recognized for its ability to access rural and underserved populations. The advent of COVID-19 in March 2020 limited the feasibility of in-person healthcare visits which in turn increased telehealth demand and use. However, the long-term impacts of COVID-19 on the telehealth sector of the healthcare industry, and particularly on pediatric healthcare volume demand and subsequent expansion, are yet to be determined.

Objective and Methods: To understand the impact of COVID-19 on telehealth utilization, volume demand, and expansion in one large pediatric healthcare system serving greater Dallas-Fort Worth, Texas, data on telehealth clinic visits by month, pre-COVID and post/current-COVID were compared. A quasi-experimental pretest-posttest design analysis compared telehealth visit counts from 54 ambulatory pediatric health specialties. Pre-post new patient counts were also analyzed via chi square.

Results: Total telehealth visit counts significantly increased between March–October 2019 (2,033 visits) compared to March-October 2020 (54,276 visits). Mean monthly telehealth visits increased by 6,530 visits, or 2,569.75% over the same time period (p < 0.0001). In October 2020, total telehealth visits were still 1,194.78% above 2019 levels (345 visits in 2019 vs. 4467 visits in 2020).

Discussion: Results here show a substantial volume increase in telehealth-delivered pediatric healthcare and resource utilization as a response to COVID-19. This provides a template for permanent adoption of pediatric telehealth delivery post pandemic. Further investigation is needed to determine impacts upon resource allocation, processes, and general models and standard of care to assist facilities and programs to better address the needs of the pediatric populations they serve in the post-COVID era.
Case Report: Two Newly Diagnosed Patients With KBG Syndrome—Two Different Molecular Changes
Authors: Wojciechowska Katarzyna , Nurzyńska-Flak Joanna , Styka Borys , Kacprzak Magdalena , Lejman Monika
Published: September 2021

Mutations or deletions of ANKRD11 gene are responsible for the symptoms of KBG syndrome. The KBG syndrome is a rare genetic disorder which is inherited in an autosomal dominant manner. Affected patients usually have characteristic facial features, macrodontia of the upper central incisors, hand abnormalities, developmental delay and short stature. In the present article we would like to report a clinical and molecular case study of two patients affected by KBG syndrome. The diagnosis of the first patient was confirmed by the identification of the novel pathogenic variant in ANKRD11 gene by next-generation sequencing. The second patient was diagnosed after the detection of a 16q24.2q24.3 deletion encompassing the ANKRD11 gene microarray.
Modified Dual Hepatic Vein Anastomosis in Pediatric Living-Donor Liver Transplantation Using Left Lateral Segment Grafts With Two Wide Orifices
Authors: Hou Yuchen , Wan Ping , Feng Mingxuan , Qiu Bijun , Zhou Tao , Zhu Jianjun , Luo Yi , Zhang Jianjun , Xia Qiang
Published: September 2021

Background: The anatomic variation of hepatic vein in the left lateral segment (LLS) increases the risk of outflow complication in pediatric living liver transplantation (LDLT). Here, we share a modified method for dual hepatic vein reconstruction in pediatric LDLT using LLS with two wide orifices.

Methods: From Sep 2018 to Dec 2019, 434 pediatric LDLTs using LLS were performed in our center. Hepatic veins of grafts were classified into three types with emphasis on the number, size, and location of orifices at the cut surface: a single opening (type I, n = 341, 78.57%); two adjacent orifices (type II, n = 66, 15.21%); two wide orifices with orifices distances <20 mm (type IIIa, n = 15, 3.46%); and two wide orifices with orifices distances >20 mm (type IIIb, n = 12, 2.76%). Rv was defined as the ratio of diameter of V2 and V3 (refer to hepatic vein drained segments II and III). We developed a modified dual hepatic vein anastomosis to reconstruct outflow for type IIIb grafts with Rv ≤1. Briefly, the hepatic vein of segment II was anastomosed to the common stump of middle hepatic vein (MHV) and left hepatic vein (LHV), followed by unification of V3 and the longitudinal incision orifice in inferior venous cave (IVC).

Results: During median follow-up of 15.6 months (7.5–22.9 months), no hepatic vein complications occurred.

Conclusion: This novel modified dual hepatic vein anastomosis could serve as a feasible surgical option for type IIIb LLS grafts with Rv ≤1 in pediatric LDLT.
Safety, Tolerability, and Pharmacokinetics of a Novel Mitochondrial Modulator, TRC150094, in Overweight and Obese Subjects: A Randomized Phase-I Clinical Trial
Authors: Joshi Deepa , Jamadarkhana Prashant , Kumbhare Suchit , Singh Amarinder , Kotecha Jignesh , Bunger Deepak , Shiwalkar Ajay , Mohanan Anookh , Dutt Chaitanya
Published: September 2021

TRC150094, a novel mitochondrial modulator, can restore metabolic flexibility by improving insulin resistance in preclinical studies. This study primarily aims to evaluate the safety, tolerability, and pharmacokinetics (PK) of oral TRC150094 after conducting two double-blind, randomized, Phase-I studies, single ascending dose (SAD) and multiple ascending dose (MAD), with n = 46, in overweight/obese adult and elderly subjects. In addition, the effect of TRC150094 on pharmacodynamic (PD) efficacy markers was evaluated. PK assessments, including maximum concentration (Cmax), area under the plasma concentration (AUC), time to Cmax (Tmax), and elimination half-life (t½), were assessed at pre-specified time points. PD assessments included apolipoprotein B (ApoB), triglycerides, hepatic fat by magnetic resonance spectroscopy (MRS) and cardiopulmonary exercise testing (CPET) parameters. TRC150094 was rapidly absorbed, and the AUC of TRC150094 increased in a dose-dependent manner across all doses in non-elderly and elderly cohorts. Cmax was more than the dose-proportional for all doses in all cohorts. Tmax ranged from 0.25 to 4 h, and t½ ranged from 15 to 18 h, making TRC150094 suitable for once-daily dosing. Food did not interfere with the overall absorption of the drug. The metabolites of TRC150094 were glucuronide and sulfate conjugates, and 20% of the drug was excreted unchanged in the urine. TRC150094 at 50 mg showed an improving trend in triglycerides. A significant reduction in Apo B was observed after 50 mg dose (−2.34 vs. 13.24%, p = 0.008), which was, however, not the case after 150 mg (8.78 vs. 13.24%, p = 0.1221). Other parameters such as hepatic fat and insulin sensitivity indices (HOMA-IR, MATSUDA Index derived from OGTT) showed an improving trend for the dose of 50 mg. In terms of safety, all the AEs reported were mild to moderate in severity. None of the adverse events was considered definitely or probably related to treatment, and there were no abnormal laboratory findings. In conclusion, the PK of TRC150094 was linear with no clinically significant food effect. TRC150094 and its metabolites suggest a lesser likelihood of drug-drug interactions. Overall, TRC150094 ensured safety and exhibited suitability for all subjects.

Clinical Trial Registration: EUDRA CT: 2009-014941-10 (SAD) and CTR-India registration: CTRI/2009/091/000601 (MAD)
Immunotherapy Summary for Cytokine Storm in COVID-19
Authors: Li Yaqun , Zhao Wenjie , Liu Jinhua , Chen Zichao , Lv Qingtao , Zhang Zhen
Published: September 2021

COVID-19 pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has ravaged the world, resulting in an alarming number of infections and deaths, and the number continues to increase. The pathogenesis caused by the novel coronavirus was found to be a disruption of the pro-inflammatory/anti-inflammatory response. Due to the lack of effective treatments, different strategies and treatment methods are still being researched, with the use of vaccines to make the body immune becoming the most effective means of prevention. Antiviral drugs and respiratory support are often used clinically as needed, but are not yet sufficient to alleviate the cytokine storm (CS) and systemic inflammatory response syndrome. How to neutralize the cytokine storm and inhibit excessive immune cell activation becomes the key to treating neocoronavirus pneumonia. Immunotherapy through the application of hormones and monoclonal antibodies can alleviate the immune imbalance, but the clinical effectiveness and side effects remain controversial. This article reviews the pathogenesis of neocoronavirus pneumonia and discusses the immunomodulatory therapies currently applied to COVID-19. We aim to give some conceptual thought to the prevention and immunotherapy of neocoronavirus pneumonia.
Mechanism of Ulcerative Colitis-Aggravated Liver Fibrosis: The Activation of Hepatic Stellate Cells and TLR4 Signaling Through Gut-Liver Axis
Authors: Liu Yu-Feng , Niu Guo-Chao , Li Chen-Yang , Guo Jin-Bo , Song Jia , Li Hui , Zhang Xiao-Lan
Published: September 2021

Background:The progression of liver disorders is frequently associated with inflammatory bowel disease through the gut-liver axis. However, no direct evidence showed the mechanisms of ulcerative colitis (UC) in the development of liver fibrosisper se. Thus, this study aimed to evaluate the effects of UC on liver fibrosis and its potential mechanism in the experimental model.

Methods:Male C57BL/6 mice were allocated into five groups (n= 10 per group) to receive either drinking water (control), 2% dextran sulfate sodium (DSS), olive oil, carbon tetrachloride (CCl4) or DSS + CCl4for 4 cycles. Blood was collected for biochemical analysis. Colons were excised for the evaluation of colon length and morphological score. Liver, colon, and mesenteric lymph nodes (MLNs) were collected for histopathological staining, expression analysis, and bacterial translocation assay to evaluate the inflammation, fibrosis, the activation of hepatic stellate cells (HSCs), and gut barrier function.

Results:DSS caused severe colitis in mice treated or treated with CCl4, as evident from the elevation of disease activity index (DAI), histological abnormalities, and increased pro-inflammatory cytokines (TNF-α, IFN-γ, and IL-17A). Histopathological staining revealed that DSS treatment aggravated the CCl4-induced extracellular matrix deposition, liver fibrosis, and inflammation in mice. Additionally, biochemical and expression analysis indicated the DSS treatment caused the increase of hydroxyproline and pro-inflammatory cytokines, as well as the abnormal liver function indexes in CCl4-induced mice. Gut barrier function was impaired in DSS- and DSS + CCl4-treated mice, manifesting as the increase in bacterial translocation and lipopolysaccharide level, and the reduction in tight junction proteins (occluding, claudin-1 and ZO-1) expression. Further, the activations of HSCs and TLR4 signaling pathway were observed after DSS + CCl4treatment, presenting with the increase in expression of α-SMA, vimentin, TGF-β, collagen type I, collagen type II, TIMP-2, TLR4, TRAF6, and NF-κB p65, and a decrease in GFAP and MMP-2 expression.

Conclusion:The present study verified that UC aggravated CCl4-induced liver injury, inflammation, and fibrosis in mice through the gut-liver axis. Gut barrier dysfunction in UC leads to bacterial translocation and elevated lipopolysaccharide, which may promote the activation of TLR4 signaling and HSCs in the liver.
The Relationship Between Perceived Stress, State-Trait Anxiety, and Sleep Quality Among University Graduates in China During the COVID-19 Pandemic
Authors: Liu Bailong , Qiao Ke , Lu Youfeng
Published: September 2021

The study aimed to investigate the relationship among perceived stress, state-trait anxiety, and sleep quality of graduates to provide a reference for improving their psychological status and attitude adjustment of job-searching during the COVID-19 pandemic. The research was conducted in a descriptive cross-sectional online survey between May 2020 and August 2020. The data were collected from 1,200 participants by using the personal information form prepared by the researchers in line with the literature, the Perceived Stress Scale, the State-Trait Anxiety Inventory, and the Pittsburgh Sleep Quality Index (PSQI). Among the surveyed participants, 47.67% were female, and 10.92% were medical students. The mean perceived stress, state anxiety, trait anxiety, and sleep quality were moderate and found as 31.4±6.69, 46.67±5.80, 49.45±5.54, and 5.94±2.47, respectively. The detection rates of state anxiety and trait anxiety were 48.63 and 49.50%, respectively. There was no significant difference in the detection rate of state anxiety and trait anxiety among different genders and majors (p >0.05). The detection rate of state anxiety and trait anxiety of rural family students was higher than that of urban family students (p <0.01). The score on the PSQI was positively associated with the scores on the perceived stress, state anxiety, and trait anxiety scales (p <0.001 for each model). Sleep quality was associated with increased perceived stress, state anxiety, and trait anxiety among graduates in China. Collectively, the study revealed the relationship between perceived stress, state-trait anxiety, and sleep quality among university graduates in China during the COVID-19 pandemic. Our results offer novel practical implications for all circles of the society to ensure students’ health under the context of the COVID-19 epidemic.
How Does the Chinese Government Select and Funding High-Level Talents? An Empirical Study Based on the Resumes of Talents
Authors: Wu Fan , Su Jialin , Zeng Jingjing
Published: September 2021

Based on the textual data of the resumes of 499 high-level talents, this study attempts to explore the factors affecting the selection and funding of high-level talents in western China. From the empirical analysis, we found that (1) the western Chinese government tends to favor the young and native talents, with a high initial academic degree (the degree obtained before working) and final academic degree (the highest degree obtained); (2) the talents with more experience, higher education, national talent titles, and participation in national projects are more likely to receive higher levels of funding; (3) it is easier for talents in universities and research institutes to be entitled as high-level talents and to gain funding than those in enterprises; and (4) talents in the fields of medicine, agronomy, and basic sciences are more likely to be entitled as high-level talents than those in other professional fields.
Teacher Expectations and Parental Stress During Emergency Distance Learning and Their Relationship to Students’ Perception
Authors: Garrote Ariana , Niederbacher Edith , Hofmann Jan , Rösti Ilona , Neuenschwander Markus P.
Published: September 2021

School closures in spring 2020 caused by the COVID-19 pandemic were an unprecedented and drastic event for students, parents, and teachers. The unplanned adaptation of classroom instruction to emergency distance learning was necessary to ensure continued education. In this new learning environment, teachers formed expectations for student academic achievement gains, which in turn affected the opportunities for students to learn. Parents faced new challenges in supporting their children’s learning. According to parenting stress models, such drastic events can be a stress factor for parents, which in turn affects their children’s adjustment. This study analyzed the extent to which parents and teachers affected the perceptions of students in compulsory school toward distance learning through processes at home (individual level) and at the class level with data from multiple informants. On an individual level, the relationship between parents’ perceived threat of COVID-19 and their stress due to distance learning and students’ perceived threat of COVID-19 and their perception of distance learning were examined. Students’ learning behavior was accounted for as a variable related to their perception of distance learning. At the class level, the explanatory character of teacher expectations and class-aggregated achievement gains were examined. Data on students in grades 4 to 8, parents, and teachers in Switzerland were collected with standardized online questionnaires after the period of school closures. A subsample of 539 students, 539 parents, and 83 teachers was analyzed. The results of multilevel structural equation modeling suggested that students had a more positive perception of distance learning if they were able to learn more autonomously (i.e., more motivated and concentrated than in regular classroom instruction) and if their parents felt less stressed in the distance learning setting. Parents were more stressed if they perceived COVID-19 as a threat. Students’ perception of the COVID-19 threat was related to their parents’ perception but did not explain students’ learning behavior. At the class level, if teachers expected high academic achievement gains in distance learning, the average academic achievement gains of a class were greater. The greater the achievement gains were, the more positive the collective student perception of distance learning was.
A Participatory Interior Design Approach for a Restorative Work Environment: A Research-Intervention
Authors: Pasini Margherita , Brondino Margherita , Trombin Rita , Filippi Zeno
Published: September 2021

Exposure to environmental stressors has physical and psychological consequences. A demanding physical environment involves the allocation of additional attentional resources and an increase in psycho-physical stress. This study illustrates the process of a research-intervention aimed at designing a workplace, using a participatory design approach, and considering the beneficial effect of restorative environments in reducing stressful elements and improving well-being at work. Stressful situations occur daily, compromising proper functioning while causing the occurrence of physiological and/or psychological disorders. To be able to safeguard their psycho-physical well-being, people normally adopt coping strategies, i.e., remedies that allow them to cope and manage situations that generate stress. One of these strategies is the exposure to natural environments, which promotes recovery and sustains psycho-physical well-being. The restorative properties of natural environments have been scientifically proven. However, even built spaces can be thought of as restorative environments, in particular when certain conditions are granted. An applied science, known as biophilic design, provides useful indications from this perspective. This project involved 57 employees of the Italian site of an international non-governmental organization, in the transition from a site no longer adequate to a new site requiring renovation. In a first phase, a survey was conducted, to verify the perceived quality of the current workplace and to detect the unmet workers' needs, and to assess some other important psychological constructs connected with perception of restorativeness and well-being. In a second phase, the findings emerged from the survey was analyzed in depth through a participatory interior design process, together with an interdisciplinary team of architects, technicians of the organization and environmental psychology researchers. The team, together with some representatives of employees, worked together through possible scenarios, adopting a biophilic design approach, to design the new workplace. At the end, the same survey of the first phase was conducted, to detect differences in perceived quality in the new workplace compared to the previous one.
Subtle Signals of Status on Social Network Sites: Evidence From China
Authors: Wang Wangshuai , Shao Tiantian , Yi Yanxi , Fang Shijiao , Song Jingyi , Yu Zucheng
Published: September 2021

Social network sites (SNS) have been indispensable channels for people to access information, present themselves, and conduct commercial activities. Existing literature on online consumer behavior mainly focus on Western consumers and on explicit conspicuous signals. However, reports have shown that SNS users in China have exceeded 370 million, ranking the first in the world. Meanwhile, more and more consumers display status in more implicit ways nowadays. To fill these gaps, the present research was conducted to investigate the subtle signals of status for Chinese consumers on SNS. We proposed that frequent SNS posting leads to higher status perception among Chinese consumers. The psychological process of this effect is perceived busyness. These hypotheses received convergent support in a set of three studies. Study 1 used secondary data to preliminarily verify the positive correlation between SNS posting frequency and perceived social status. Studies 2A and 2B adopted the causal chain method to test the underlying mechanism of the effect, and to provide causal evidence for the entire relationship chain. Specifically, Study 2A examined how SNS posting frequency affects perceived busyness. Furthermore, Study 2B explored whether the differences in perceived busyness will affect social status perceptions. Implications of these findings and potential extensions in future are discussed.
The Sichuan Mental Health Survey: Methodology
Authors: Su Rong-cheng , Huang Li-hong , Li Jia , Zhou Bo , Zhao Jia-jun , Li Hui
Published: September 2021

The Sichuan Mental Health Survey (SMHS) is a provincially representative survey with a coherent methodology to obtain the prevalence of multiple mental disorders and data of services used and to analyze the psychological and social risk factors or correlates in Sichuan, China. Mental disorders include anxiety disorders, mood disorders, schizophrenia, and other psychotic disorders, drug use and alcohol use disorders, impulse control disorder, and eating disorders. A cross-sectional design is employed to sample adults from 200 communities/villages in all 21 prefectural-level municipalities of Sichuan Province in a five-stage provincially representative disproportionate stratified sampling design. The participants need to be interviewed face to face by trained interviewers from local primary healthcare institutions and by psychiatrists. The quality control staff implement data quality control by checking records and statistics in the interview system, and then re-interviewing checks are done by the psychiatrists. Data is weighted to adjust the sample distribution to match the whole population. The outcomes of the SMHS would not only demonstrate the serious challenges posed by the high burdens of mental disorders but also offer baseline data for policymakers and healthcare professionals to study and resolve the factors that influence mental health in Sichuan, China.
A Systematic Review of Human and Robot Personality in Health Care Human-Robot Interaction
Authors: Esterwood Connor , Robert Lionel P.
Published: September 2021

Robots have become vital to the delivery of health care and their personalities are often important to understanding their effectiveness as health care providers. Despite this, there is a lack of a systematic overarching understanding of personality in health care human-robot interaction. This makes it difficult to understand what we know and do not know about the impact of personality in health care human-robot interaction (H-HRI). As a result, our understanding of personality in H-HRI has not kept pace with the deployment of robots in various health care environments. To address this, the authors conducted a literature review that identified 18 studies on personality in H-HRI. This paper expands, refines, and further explicates the systematic review done in a conference proceedings [see: Esterwood (Proceedings of the 8th International Conference on Human-Agent Interaction, 2020, 87–95)]. Review results: 1) highlight major thematic research areas, 2) derive and present major conclusions from the literature, 3) identify gaps in the literature, and 4) offer guidance for future H-HRI researchers. Overall, this paper represents a reflection on the existing literature and provides an important starting point for future research on personality in H-HRI.
Oral Microbiome and Gingival Gene Expression of Inflammatory Biomolecules With Aging and Periodontitis
Authors: Ebersole Jeffrey L. , Nagarajan Radhakrishnan , Kirakodu Sreenatha , Gonzalez Octavio A.
Published: September 2021

Although data describe the presence and increase of inflammatory mediators in the local environment in periodontitis vs. health in humans, details regarding how these responses evolve in the transition from health to disease, changes during disease progression, and features of a resolved lesion remain unknown. This study used a nonhuman primate model of ligature-induced periodontitis in young, adolescent, adult, and aged animals to document features of inflammatory response affected by age. Rhesus monkeys had ligatures tied and provided gingival tissue biopsy specimens at baseline, 0.5, 1, and 3 months of disease and at 5 months of the study, which was 2 months post-ligature removal for clinically resolved tissues. The transcriptome was assessed using microarrays for chemokine (n = 41), cytokine (n = 45), chemokine receptor (n = 21), cytokine receptor (n = 37), and lipid mediator (n = 31) genes. Limited differences were noted in healthy tissues for chemokine expression with age; however, chemokine receptor genes were decreased in young but elevated in aged samples. IL1A, IL36A, and IL36G cytokines were decreased in the younger groups, with IL36A elevated in aged animals. IL10RA/IL10RB cytokine receptors were altered with age. Striking variation in the lipid mediator genes in health was observed with nearly 60% of these genes altered with age. A specific repertoire of chemokine and chemokine receptor genes was affected by the disease process, predominated by changes during disease initiation. Cytokine/cytokine receptor genes were also elevated with disease initiation, albeit IL36B, IL36G, and IL36RN were all significantly decreased throughout disease and resolution. Significant changes were observed in similar lipid mediator genes with disease and resolution across the age groups. Examination of the microbiome links to the inflammatory genes demonstrated that specific microbes, including Fusobacterium, P. gingivalis, F. alocis, Pasteurellaceae, and Prevotella are most frequently significantly correlated. These correlations were generally positive in older animals and negative in younger specimens. Gene expression and microbiome patterns from baseline were distinctly different from disease and resolution. These results demonstrate patterns of inflammatory gene expression throughout the phases of the induction of a periodontal disease lesion. The patterns show a very different relationship to specific members of the oral microbiome in younger compared with older animals.
How Oligodendrocytes Help The Brain Function
Authors: Suter Tracey A. C. S. , He Zhigang
Published: September 2021

Imagine you had a really important message that you had to deliver to your friend as quickly as possible. Would you send your friend a handwritten letter through the postal service that would arrive in a few days? Or would you send an email, which gets delivered almost instantly? A neuron’s job is to speedily send messages across the brain, spinal cord, and body. Neurons receive help with performing this important job from another kind of cell, called oligodendrocytes. In this article, we describe how oligodendrocytes help neurons rapidly send their messages, and how scientists are trying to protect and replace oligodendrocytes that are damaged in diseases like multiple sclerosis, or after a brain injury.
The Rapid Data-Driven Prediction Method of Coupled Fluid–Thermal–Structure for Hypersonic Vehicles
Authors: Liu Jing , Wang Meng , Li Shu
Published: September 2021

This work demonstrates the use of Latin Hypercube Sampling and Proper Orthogonal Decomposition in combination with a Radial Basis Function model to perform on vehicle prediction coupled fluid–thermal–structure. We regarded the Mach number, flight altitude and angle of attack as input parameters and established a rapid prediction model. The basic process of numerical simulation of the hypersonic vehicle coupled fluid–thermal–structure was studied to obtain the database of pressure coefficient, heat flux, structural temperature and structural stress as the sample data to train this prediction method. The prediction error was analyzed. The prediction results showed that the data-driven method proposed in this paper based on proper orthogonal decomposition and radial basis function could be used for predicting vehicle coupled fluid–thermal–structure with good efficiency.
Proper Technical Maintenance of Combine Harvester Rolling Bearings for Smooth and Continuous Performance for Grain Crop Agrotechnical Requirements
Authors: Jotautienė Eglė , Juostas Antanas , Bhandari Shankar
Published: September 2021

The threshing mechanism is the main component of the combine harvester on which the grain separation and cleaning qualitative work indicators depend. It is important to ensure that all threshing mechanism components, including the threshing drum bearings and all other bearings of the combine, are working properly and reliably. There are many places in the combine where it is not possible to measure bearing vibrations directly without dismounting them, since there is no suitable spot to mount a sensor. The paper investigates the threshing drum rolling bearing condition of combines, which are difficult to access, by using a vibration diagnostics technique utilizing a newly manufactured steel bracket. The vibration measurements and analysis were conducted by the Adash A4900 Vibrio M analyzer (Adash spol. s.r.o., Ostrava, Czech Republic). The vibration source measurement was based on the fast Fourier transform (FFT) spectrum analysis. Analysis of the experimental results showed that average squared velocity values (in the frequency interval of 10–1000 Hz), together with other measured vibration parameters, can be used for the combine threshing drum‘s bearing condition evaluation.
Cytochrome 4Z1 Expression Is Correlated with Poor Prognosis in Patients with Cervical Cancer
Authors: Al-saraireh Yousef M. , Alshammari Fatemah O. F. O. , Youssef Ahmed M. M. , Al-sarayra Yahya M. , Al-saraireh Renata A. , Al-muhaisen Ghadeer H. , Al-mahdy Yanal S. , Al-Kharabsheh Ahlam M. , Abufraijeh Seham M. , Alrawashdeh Hamzeh Mohammad
Published: September 2021

Background: cervical cancer is one of the most common malignancies in women worldwide and its management remains challenging and complex. As Cytochrome4Z1 (CYP4Z1) is overexpressed in many tumours, its expression in cervical cancer is unknown. Therefore, the present study aimed to evaluate CYP4Z1 expression in cervical cancers. Methods: CYP4Z1 expression was immunohistochemically assessed in 100 cases of cervical cancers along with ten normal cervix tissues, and the enzyme’s relationship to several clinicopathological features and survival was explored. Results: CYP4Z1 was strongly expressed in 55% of cervical cancer patients. Normal cervix samples were negative for CYP4Z1 expression. Importantly, this expression was significantly found in patients with the late stage of the disease, lymph node metastasis, and high tumour invasion (p < 0.05). Interestingly, CYP4Z1 expression was significantly correlated with shorter survival times of cervical cancer patients. Univariate analysis showed that CYP4Z1 expression, tumour stage, lymph node metastasis, and tumour invasion were significantly correlated with patient survival (p < 0.05). The multivariate analysis revealed that only CYP4Z1 expression and tumour stage were significantly correlated with patient survival (p < 0.05). Conclusions: CYP4Z1 expression is associated with cervical cancer patients’ survival and may serve as an independent predictor of poor prognosis in cervical cancer patients.
Learning in Convolutional Neural Networks Accelerated by Transfer Entropy
Authors: Moldovan Adrian , Caţaron Angel , Andonie Răzvan
Published: September 2021

Recently, there is a growing interest in applying Transfer Entropy (TE) in quantifying the effective connectivity between artificial neurons. In a feedforward network, the TE can be used to quantify the relationships between neuron output pairs located in different layers. Our focus is on how to include the TE in the learning mechanisms of a Convolutional Neural Network (CNN) architecture. We introduce a novel training mechanism for CNN architectures which integrates the TE feedback connections. Adding the TE feedback parameter accelerates the training process, as fewer epochs are needed. On the flip side, it adds computational overhead to each epoch. According to our experiments on CNN classifiers, to achieve a reasonable computational overhead–accuracy trade-off, it is efficient to consider only the inter-neural information transfer of the neuron pairs between the last two fully connected layers. The TE acts as a smoothing factor, generating stability and becoming active only periodically, not after processing each input sample. Therefore, we can consider the TE is in our model a slowly changing meta-parameter.
Storage and Upgrading of Biogas by Physicochemical Purification in a Sudano-Sahelian Context
Authors: Djomdi  , Mintsop Nguela Leonel Junior , Bakari Hamadou , Fadimatou Hamadou , Christophe Gwendoline , Michaud Philippe
Published: September 2021

The global energy trends are currently dominated by a massive use of fossil non-renewable energy sources which are progressively depleting. In this way, the production of second-generation biogas production from organic wastes by the dark fermentation process offers, therefore, an attractive solution to diversify the present energy mix. The development of biogas production units has led to an increase in the quantity of biomethane, but it contains impurities. A biomethane purification and storage system was developed in this work to improve the quality of this biofuel. Solutions were first developed to capture carbon dioxide, hydrogen sulfide, water, and volatile organic compounds found in the initial biogas. These solutions were based on a system of purification made up of water absorption reactions and iron oxide, activated charcoal, and steel wool adsorption. Thus, the biomethane obtained after purification has been stored in an inflatable balloon before being compressed into a refrigerant bottle of R134a. The treatment system was used to release a biogas with 95 % biomethane and a law heating value (LHV) of 54 MJ/kg after purification. It also emerges that purification of 2 m3 of biogas requires 0.15 m3 of water at 20oC to produce 1.4 m3 of biomethane. This biomethane can meet an energy demand of 1624 Wh or 0.2 m3 of daily biomethane requirements. The system as a whole can allow customers with a biodigester to produce their own energy (cooking or electric) while reducing the production of green-house gases in the atmosphere.
Energy Recovery in Air Conditioning Systems: Comprehensive Review, Classifications, Critical Analysis, and Potential Recommendations
Authors: Aridi Rima , Faraj Jalal , Ali Samer , Gad El-Rab Mostafa , Lemenand Thierry , Khaled Mahmoud
Published: September 2021

Energy has become the backbone of humanities daily activities. Heating, ventilating, and air conditioning systems (HVAC), which consume around 39% of energy in the residential sector, have turned into an essential constituent for providing fresh air, especially after COVD-19, not only in hospitals but also in any simple construction. Thus, decreasing this percentage or recovering part of the energy lost is an essential issue in today’s energy management scenarios. In this context, the present manuscript suggests a comprehensive review, classifications, critical analysis, and potential recommendations for energy recovery in air conditioning systems. It classifies energy recovery into two main categories: using lost energy for external uses, such as heating domestic water, or with other devices; and using lost energy for internal uses, such as the hot airflow which can be reused again for increasing efficiency of HVAC. In addition, this paper presents a summary of previous research and undertakes a review of the devices used for recovering energy. Furthermore, this review identifies superior devices in terms of climate and weather conditions. These objectives are accomplished by investigating around 190 published papers to conclude that energy recovery devices show a considerable effect on energy consumption in HVAC, mainly the heat pipe, fixed plate, and rotary wheel devices.
High Tunnel Production of Containerized Hybrid and Heirloom Tomatoes Using Grafted Plants with Two Types of Rootstocks
Authors: Arthur Jacob Dale , Li Tongyin , Lalk Geoffrey Thomas , Bi Guihong
Published: September 2021

Tomatoes (Solanum lycopersicum) are not only one of the most widely grown and consumed vegetables in the U.S., but are also one of the most economically important vegetables for Mississippi growers operating on small- to medium-sized farms. High tunnel production and vegetable grafting serve as effective approaches to provide season extension and improve productivity and resistance to a number of abiotic and biotic factors for tomato plants. Six tomato cultivars, including three hybrids (‘Big Beef’, ‘Early Girl’, and ‘Sun Sugar’) and three heirlooms (‘Brandywine’, ‘Mortgage Lifter’, and ‘San Marzano’), were evaluated for plant growth, fruit yield, and quality in a containerized high tunnel production system in 2020. Each cultivar was grafted onto two types of interspecific hybrid rootstocks ‘Emperador’, or ‘Maxifort’, or grown non-grafted as control. ‘Big Beef’ and ‘Early Girl’ produced comparable highest marketable yields of 9.62 to 11.12 kg per plant, compared with ‘San Marzano’ and ‘Sun Sugar’ producing the lowest marketable yields of 3.27 to 4.76 kg per plant due to small fruit sizes. Grafting the selected tomato cultivars with the two rootstock types did not alter total marketable yield of any cultivar, but affected overall stem diameter, fruit color, and β-carotene concentrations. The rootstock ‘Emperador’ decreased soluble solids content and titratable acidity in ‘Early Girl’ compared to ‘Maxifort’ grafted or non-grafted plants. The high tunnel enabled early transplanting and resulted in advanced tomato harvest by approximately three to four weeks compared to local field production.
Stress Distribution in Modified Veneer Crowns: 3D Finite Element Analysis
Authors: Madruga Camila Ferreira Leite , Ramos Gabriela Freitas , Borges Alexandre Luiz Souto , Saavedra Guilherme de Siqueira Ferreira Anzaloni , Souza Rodrigo Othávio , Marinho Renata Marques de Melo , Penteado Marcela Moreira
Published: September 2021

(1) Background: to propose a new approach for crown veneers, with the use of an aesthetic porcelain coating, only in part of the zirconia infrastructure, and to analyze its biomechanical behavior to minimize chipping failures. (2) Methods: a maxillary molar was modeled using CAD software, preparing for traditional crowns and veneer crowns with various lengths. Five groups were formed: M—control group (monolithic crown of ultra-translucent zirconia); B—conventional (bilayer crown of ultra-translucent zirconia and ceramic covering); V—veneer (ultra-translucent zirconia crown with reduction only in the buccal and application of covering ceramics); V1—ultra-translucent zirconia crown with buccal reduction and 1 mm occlusal extension for covering ceramic application); V1.5—ultra-translucent zirconia crown with buccal reduction and 1.5 mm occlusal extension for application of covering ceramics. A load of 600 N was performed axially to a fossa bottom to simulate parafunction, and 300 N to the cusp tip to obtain the maximum principal stress results. (3) Results: group B showed a higher stress concentration in the occlusal region of the restorations, while the other groups absorbed the stress and dissipated it throughout the crown, presenting a higher stress concentration in the interface region with the tooth. (4) Conclusion: the highly translucent zirconia ceramic only associated with buccal covering ceramic could add aesthetic gain and rigidity to the system and could be a good option to restore maxillary molars in patients who do not have parafunction.
Association between Diabetes and Dental Caries in Spanish Adults: A Cross-Sectional Study Including 23,089 Adults
Authors: Jacob Louis , Shin Jae-Il , Grabovac Igor , Haro Josep Maria , Koyanagi Ai , Tully Mark A. , Trott Mike , Kim Jong Yeob , Pardhan Shahina , Smith Lee , López Sánchez Guillermo F.
Published: September 2021

The aim of the study was to investigate the cross-sectional association between diabetes and dental caries in a representative sample of 23,089 adults residing in Spain. Data from the Spanish National Health Survey 2017 were analysed. Diabetes (independent variable) and dental caries (dependent variable) were evaluated through a self-reported questionnaire. The association between diabetes and dental caries in the overall sample was assessed using logistic regression models adjusted for sex, age, marital status, education, smoking, alcohol consumption, obesity, hypertension, and hypercholesterolemia. The prevalence of dental caries was 20.6% in the overall population and was significantly higher in men and in young (≤40 years) and middle-aged adults (41–65 years) than in women and older adults (>65 years), respectively. After adjusting for control variables, there was a positive and significant association between diabetes and dental caries in the overall population (OR = 1.30, 95% CI = 1.15–1.46). The relationship between diabetes and dental caries was particularly strong in women (OR = 1.45, 95% CI = 1.22–1.71) and in adults aged ≤40 years (OR = 1.80, 95% CI = 1.05–3.05). In conclusion, in this large representative sample of Spanish adults, diabetes was associated with having a higher prevalence of dental caries, with females and younger adults at greatest risk. Patients with diabetes and dental practitioners should be aware of these associations and act accordingly. Future research should aim to investigate the mediating factors involved in the observed association between diabetes and dental caries.
Sustainable land use in Crimea
Authors: Melnichuck A.Yu. , Zackalichnaya O.V.
Published: September 2021

The article deals with the formation of a sustainable land use system. It is proposed to put land zoning as the basis for the formation of a sustainable land use system, reflecting the territory difference by soil, morphometric and agroclimatic conditions. The zoning of the lands of the Simferopol region was completed according to their suitability for growing grapes. The infrastructure of the regional information and analytical system of sustainable land use has been developed for this area. The authors proposed an algorithm for the formation of sustainable land use on the platform of digital land management and land use.
Sea Mayweed Tripleurospermum maritimum and Scentless Mayweed T. inodorum (Asteraceae) intermediates in Orkney
Authors: Crossley John , Skilbeck Christopher A.
Published: September 2021

This article describes a study of Tripleurospermum maritimum (L.) W.D.J. Koch and T. inodorum (L.) Sch. Bip. (Asteraceae) in the Orkney Islands (v.c.111), the results of which suggest that intermediates between these taxa may be rather common, and that T. maritimum subsp. nigriceps and subsp. maritimum are both involved, the former more frequently. Obviously this results in a complex taxonomic situation, evidently not confined to Orkney in the far north. Key identifying characters of the taxa are systematically examined and guidance offered on determining hybrids using a population level approach. The taxonomic complexities of these northern populations are discussed, with regard in particular to the identity of T. inodorum occurring there and the place of T. maritimum subsp. nigrescens in the forms and subspecies of T. maritimum found in the north Atlantic region.