bluuebunny/crossref_metadata_2025 huggingface dataset:
Water-Cycle-Algorithm-Tuned Intelligent Fuzzy Controller for Stability of Multi-Area Multi-Fuel Power System with Time Delays
Authors: Kalyan CH. Naga Sai , Goud B. Srikanth , Bajaj Mohit , Kumar Malligunta Kiran , Ahmed Emad M. , Kamel Salah
Published: February 2022

In this paper, a fuzzy (F) proportional (P)–integral (I)–derivative (D) (PID) (FPID) controller optimized with a water cycle algorithm is proposed for load frequency control of a multi-area multi-fuel (MAMF) power system. The MAMF system has the realistic feature of communication time delays (CTDs), in order to conduct an analysis nearer to realistic practice. Initially, the MAMF system is analyzed when subjected to a step load disturbance (SLD) of 10% on area 1. The superiority of the fuzzy PID controller is revealed upon comparing it with PID plus double derivative (DD) (PIDD) and PID controllers. The MAMF system is investigated with and without CTDs, to demonstrate their impact on system performance. Later, an additional HVDC line is incorporated in parallel with the existing AC line for further enhancement of the system performance. Finally, the MAMF system is targeted with random loading to validate the robustness of the presented control scheme.
Secure Data Transmission and Image Encryption Based on a Digital-Redesign Sliding Mode Chaos Synchronization
Authors: Fang Jiunn-Shiou , Tsai Jason Sheng-Hong , Yan Jun-Juh , Chiang Li-Huseh , Guo Shu-Mei
Published: February 2022

In this paper, a novel image encryption algorithm based on chaotic synchronization is proposed. First, a digital-redesign sliding mode controller (SMC) is developed to guarantee the chaos synchronization. The digital redesign method makes it possible to transform a proposed continuous-time SMC to discrete-time SMC whilst maintaining the performance of the robust synchronization. Then, the secret keys are embedded in the state equations of the master chaotic system, such that the secret keys do not appear in the public channel, and utilize the chaotic synchronization to achieve secure communication for transmitting the secret keys from transmitter to receiver. Second, an image encryption algorithm integrating the S-box with chaotic synchronization is established, where the S-box is created by the secret key transmitted from the transmitter. Finally, a detailed analysis of the image encryption algorithm based on chaos synchronization is included to verify the feasibility and effectiveness of this proposed approach.
Identification of Homogeneous Groups of Actors in a Local AHP-Multiactor Context with a High Number of Decision-Makers: A Bayesian Stochastic Search
Authors: Altuzarra Alfredo , Gargallo Pilar , Moreno-Jiménez José María , Salvador Manuel
Published: February 2022

The identification of homogeneous groups of actors in a local AHP-multiactor context based on their preferences is an open problem, particularly when the number of decision-makers is high. To solve this problem in the case of using stochastic AHP, this paper proposes a new Bayesian stochastic search methodology for large-scale problems (number of decision-makers greater than 20). The new methodology, based on Bayesian tools for model comparison and selection, takes advantage of the individual preference structures distributions obtained from stochastic AHP to allow the identification of homogeneous groups of actors with a maximum common incompatibility threshold. The methodology offers a heuristic approach with several near-optimal partitions, calculated by the Occam’s window, that capture the uncertainty that is inherent when considering intangible aspects (AHP). This uncertainty is also reflected in the graphs that show the similarities of the decision-maker’s opinions and that can be used to achieve representative collective positions by constructing agreement paths in negotiation processes. If a small number of actors is considered, the proposed algorithm (AHP Bayesian clustering) significantly reduces the computational time of group identification with respect to an exhaustive search method. The methodology is illustrated by a real case of citizen participation based on e-Cognocracy.
Sdy-1 Executes Antitumor Activity in HepG2 and HeLa Cancer Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway
Authors: Sun Mengyu , Zhou Dongdong , Wu Jingwan , Zhou Jing , Xu Jing
Published: February 2022

Demethylincisterol A3 (Sdy-1), a highly degraded sterol that we previously isolated from Chinese mangrove Rhizophora mucronata endophytic Pestalotiopsis sp. HQD-6, exhibits potent antitumor activity towards a variety of cancer cells. In this study, we further verified that Sdy-1 effectively inhibited the proliferation and migration of human liver (HepG2) and cervical cancer (HeLa) cells in vitro and it can induce cell apoptosis and arrest the cell cycle in the G1-phase. Mechanistically, we demonstrated that Sdy-1 executes its function via inhibition of the Wnt/β-catenin signaling pathway. Sdy-1 may not inhibit the Wnt signaling pathway through the cascade reaction from upstream to downstream, but directly acts on β-catenin to reduce its transcription level, thereby reducing the level of β-catenin protein and further reducing the expression of downstream related proteins. The possible interaction between Sdy-1 and β-catenin protein was further confirmed by molecular docking studies. In the nude mouse xenograft model, Sdy-1 can also significantly inhibit tumor growth. These results indicated that Sdy-1 is an efficient inhibitor of the Wnt signaling pathway and is a promising antitumor candidate for therapeutic applications.
Eradication of Isolated Para-Aortic Nodal Recurrence in a Patient with an Advanced High Grade Serous Ovarian Carcinoma: Our Experience and Review of Literature
Authors: Tinelli Raffaele , Dellino Miriam , Nappi Luigi , Sorrentino Felice , D’Alterio Maurizio Nicola , Angioni Stefano , Bogani Giorgio , Pisconti Salvatore , Silvestris Erica
Published: February 2022

We report a case report regarding the eradication of isolated lymph-nodal para-aortic recurrence in the aortic region down the left renal vein (LRV) in a patient treated two years earlier in another hospital for a FIGO stage IC2 high-grade serous ovarian carcinoma with a video showing the para-aortic space after eradication of the metastatic tissue. A 66 year-old woman was admitted 24 months after the initial surgical procedure for an increased Ca 125 level and CT scan that revealed a 3 cm para-aortic infrarenal lymph-nodal recurrence that was confirmed by PET/CT scan. A secondary cytoreductive surgery (SCS) with a para-aortic lymph-nodal dissection of the tissue down the LRV and radical omentectomy were performed: during the cytoreduction, the right hemicolon was mobilized. The anterior surface of the inferior vena cava (IVC), aorta and LRV were exposed. The metastatic lymph nodes were detected in the para-ortic space down the proximal part of the LRV and eradicated; an en bloc infrarenal lymph-node dissection from the aortocaval region was performed. The operative time during the surgical procedure was 212 min with a blood loss of 120 mL. No intra- and postoperative complications, including ureteral or vascular injury or renal dysfunction, occurred. At histological examination, three dissected lymph nodes were positive for metastasis, and the patient was discharged five days after laparotomy without side effects and underwent chemotherapy 3 weeks later; after a follow-up of 42 months, no recurrence was detected. In conclusion, secondary debulking surgery can be considered a safe and effective therapeutic option for the management of recurrences, although long-term follow-ups are necessary to evaluate the overall oncologic outcomes of this procedure.
Serum Uric Acid Levels in Parkinson’s Disease: A Cross-Sectional Electronic Medical Record Database Study from a Tertiary Referral Centre in Romania
Authors: Dănău Adela , Dumitrescu Laura , Lefter Antonia , Popescu Bogdan Ovidiu
Published: February 2022

Background and Objectives: Parkinson’s disease (PD) is a prevalent neurodegenerative condition responsible for progressive motor and non-motor symptoms. Currently, no prophylactic or disease-modifying interventions are available. Uric acid (UA) is a potent endogenous antioxidant, resulting from purine metabolism. It is responsible for about half of the antioxidant capacity of the plasma. Increasing evidence suggests that lower serum UA levels are associated with an increased risk of developing PD and with faster disease progression. Materials and Methods: We conducted an electronic medical record database study to investigate the associations between UA levels and different characteristics of PD. Results: Out of 274 datasets from distinct patients with PD, 49 complied with the predefined inclusion and exclusion criteria. Lower UA levels were significantly associated with the severity of parkinsonism according to the Hoehn and Yahr stage (rs = 0.488, p = 0.002), with the motor complications of long-term dopaminergic treatment (r = 0.333, p = 0.027), and with the presence of neurocognitive impairment (r = 0.346, p = 0.021). Conclusions: Oxidative stress is considered a key player in the etiopathogenesis of PD, therefore the involvement of lower UA levels in the development and progression of PD is plausible. Data on the potential therapeutic roles of elevating serum UA (e.g., by precursor administration or diet manipulation) are scarce, but considering the accumulating epidemiological evidence, the topic warrants further research.
Mathematical Modeling of the Effect of Pulsed Electric Field Mode and Solution Flow Rate on Protein Fouling during Bipolar Membrane Electroacidificaiton of Caseinate Solution
Authors: Nichka Vladlen , Mareev Semyon , Pismenskaya Natalia , Nikonenko Victor , Bazinet Laurent
Published: February 2022

A one-dimensional non-stationary model was developed for a better understanding of the protein fouling formation mechanism during electroacidification of caseinate solution using electrodialysis with bipolar membranes (EDBM) in pulsed electric field (PEF) mode. Four different PEF modes were investigated with pulse–pause durations of 10–10 s, 10–20 s, 10–33 s, 10–50 s. For each current mode 3 different flow rates were considered, corresponding to Reynolds numbers, Re, equal to 187, 374 and 560. The processes are considered in the diffusion boundary layer between the surface of the cation-exchange layer of bipolar membrane and bulk solution of the desalination compartment. The Nernst–Planck and material balance equation systems describe the ion transport. The electroneutrality condition and equilibrium chemical reactions are taken into account. The calculation results using the developed model are in qualitative agreement with the experimental data obtained during the previous experimental part of the study. It is confirmed that both the electrical PEF mode and the flow rate have a significant effect on the thickness (and mass) of the protein fouling during EDBM. Moreover, the choice of the electric current mode has the main impact on the fouling formation rate; an increase in the PEF pause duration leads to a decrease in the amount of fouling. It was shown that an increase in the PEF pause duration from 10 s to 50 s, in combination with an increase in Reynolds number (the flow rate) from 187 to 560, makes it possible to reduce synergistically the mass of protein deposits from 6 to 1.3 mg/cm2, which corresponds to a 78% decrease.
A Case Study of Calcium Carbonate Crystallization during Reverse Osmosis Water Desalination in Presence of Novel Fluorescent-Tagged Antiscalants
Authors: Popov Konstantin , Oshchepkov Maxim , Pervov Alexei , Golovesov Vladimir , Ryabova Anastasia , Trukhina Maria , Tkachenko Sergey
Published: February 2022

Calcium carbonate scaling in reverse osmosis (RO) desalination process is studied in the presence of two novel fluorescent-tagged scale inhibitors 1,8-naphthalimide-tagged polyacrylate (PAA-F1) and 1-hydroxy-7-(6-methoxy-1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)heptane-1,1-diyl-bis(phosphonic acid) (HEDP-F) by fluorescent microscopy (FM) and scanning electron microscopy (SEM). Both antiscalants diminished the mean size of calcite crystals relative to the blank experiment. The behavior and localization of HEDP-F and PAA-F1 during calcite scale formation on membrane surface was found to be significantly different from the distribution in similar RO experiments with gypsum, reported earlier. In the former case, both antiscalants are concentrated exactly on the surface of calcium carbonate crystals, while in the latter one they form their own phases (Ca-HEDP-F and Ca-PAA-F1) and are not detected on gypsum scale. The difference is interpreted in terms of interplay between background calcium concentration and sparingly soluble calcium salts’ solubility. HEDP-F reveals slightly higher efficiency than PAA-F1 against calcite scale formation, while PAA-F exhibits a higher ability to change calcite morphology. It is demonstrated that there is a lack of correlation between antiscaling efficacy and ability of antiscalant to change calcium carbonate morphology in a particular case study. An application of fluorescent-tagged antiscalants in RO experiments provides a unique possibility to track the scale inhibitor molecules’ localization during calcite scale formation. Fluorescent-tagged antiscalants are presumed to become a very powerful tool in membrane scaling inhibition studies.
Insights into Poisoning Mechanism of Zr by First Principle Calculation on Adhesion Work and Adsorption Energy between TiB2, Al3Ti, and Al3Zr
Authors: Wu Jianqiang , Ruan Qilin , Chen Simin , Meng Chuanchao , Xu Zhengbing , Wei Chunhua , Tang Hongqun , Wang Junsheng
Published: February 2022

Al-Ti-B intermediate alloys are widely used as grain refiners in aluminum alloys owing to the presence of Al3Ti and TiB2 phases. However, the existence of Zr in aluminum alloy melts often results in coarse grain size, leading to Al-Ti-B failure called Zr poisoning. There are three kinds of poisoning mechanisms related to TiB2, Al3Ti, and a combination of TiB2 and Al3Ti for Zr. First, Zr forms ZrB2 or Ti2Zr with TiB2 in Al-Ti-B to reduce the nucleation ability. Second, Zr existing in the aluminum melt with a high melting point Al3Zr then attracts Ti to reduce the dispersion of Ti as a growth inhibitor. Third, Zr reacts with Al3Ti on TiB2 surface to form Al3Zr, thereby increasing the degree of mismatch with Al and diminishing the refiner’s ability as a nucleation substrate. To gain a better understanding of the mechanism of Zr poisoning, the first principle was used in this study to calculate the adhesion works (ZrB2//Al3Ti), (Ti2Zr//Al3Ti), (Al3Zr//Al3Ti), (Al3Ti//Al), (TiB2//Al3Zr), and (Al3Zr//Al), as well as the surface energy of Al3Zr and adsorption energies of Al to Al3Ti or Al3Zr. The results demonstrated that Zr poisoning originated from the second guess. Zr element exiting in aluminum melt led to the formation of an Al3Zr (001) surface. The interfacial adhesion work of Al3Zr (001)//Al3Ti (001) was not weaker than that of TiB2//Al3Ti. As a result, Al3Zr first combined with Al3Ti to significantly decline the adsorption of Al3Ti (001) on Al, losing its role as a nucleating agent and grain coarsening. Overall, to prevent failure of the grain refiner in Zr containing aluminum melt, the adhesion work interface between the generated phase of the grain refiner and Al3Zr must remain lower to avoid the combination of the generated phase of grain refiner with Al3Zr. In sum, these findings look promising for evaluating future effects of grain refinement in Zr containing aluminum melt.
Impact of Metabolomics Technologies on the Assessment of Peritoneal Membrane Profiles in Peritoneal Dialysis Patients: A Systematic Review
Authors: Kondou Antonia , Begou Olga , Dotis John , Karava Vasiliki , Panteris Eleftherios , Taparkou Anna , Gika Helen , Printza Nikoleta
Published: February 2022

Peritoneal dialysis (PD) is an effective and frequent dialysis modality in adults, particularly preferred in infants and young children with end-stage renal disease (ESRD). Long-term exposure of the peritoneal membrane to dialysis solutions results in severe morphologic and functional alterations. Peritoneal dialysis effluent biomarkers are based on omics technologies, which could predict the onset or confirm the diagnosis of peritoneal membrane dysfunction, would allow the development of accurate early prognostic tools and, potentially, the identification of future therapeutic targets. The purpose of our study was to critically review the literature on the impact and the effectiveness of metabolomics technologies in peritoneal health. The main search was performed in electronic databases (PubMed/MEDLINE, Embase and Cochrane Central Register of Controlled Trials) from inception to December 2020, using various combinations of Medical Subject Headings (MeSH). The main search highlighted nine studies, of which seven were evaluated in detail. Metabolomics technologies may provide significant input in the recognition of peritoneal membrane dysfunction in PD patients and provide evidence of early intervention strategies that could protect peritoneum health and function.
Fecal 1H-NMR Metabolomics: A Comparison of Sample Preparation Methods for NMR and Novel in Silico Baseline Correction
Authors: Brown Catherine L. J. , Scott Hannah , Mulik Crystal , Freund Amy S. , Opyr Michael P. , Metz Gerlinde A. S. , Inglis G. Douglas , Montina Tony
Published: February 2022

Analysis of enteric microbiota function indirectly through the fecal metabolome has the potential to be an informative diagnostic tool. However, metabolomic analysis of feces is hampered by high concentrations of macromolecules such as proteins, fats, and fiber in samples. Three methods—ultrafiltration (UF), Bligh–Dyer (BD), and no extraction (samples added directly to buffer, vortexed, and centrifuged)—were tested on multiple rat (n = 10) and chicken (n = 8) fecal samples to ascertain whether the methods worked equally well across species and individuals. An in silico baseline correction method was evaluated to determine if an algorithm could produce spectra similar to those obtained via UF. For both rat and chicken feces, UF removed all macromolecules and produced no baseline distortion among samples. By contrast, the BD and no extraction methods did not remove all the macromolecules and produced baseline distortions. The application of in silico baseline correction produced spectra comparable to UF spectra. In the case of no extraction, more intense peaks were produced. This suggests that baseline correction may be a cost-effective method for metabolomic analyses of fecal samples and an alternative to UF. UF was the most versatile and efficient extraction method; however, BD and no extraction followed by baseline correction can produce comparable results.
Phage Genome Diversity in a Biogas-Producing Microbiome Analyzed by Illumina and Nanopore GridION Sequencing
Authors: Willenbücher Katharina , Wibberg Daniel , Huang Liren , Conrady Marius , Ramm Patrice , Gätcke Julia , Busche Tobias , Brandt Christian , Szewzyk Ulrich , Schlüter Andreas , Barrero Canosa Jimena , Maus Irena
Published: February 2022

The microbial biogas network is complex and intertwined, and therefore relatively stable in its overall functionality. However, if key functional groups of microorganisms are affected by biotic or abiotic factors, the entire efficacy may be impaired. Bacteriophages are hypothesized to alter the steering process of the microbial network. In this study, an enriched fraction of virus-like particles was extracted from a mesophilic biogas reactor and sequenced on the Illumina MiSeq and Nanopore GridION sequencing platforms. Metagenome data analysis resulted in identifying 375 metagenome-assembled viral genomes (MAVGs). Two-thirds of the classified sequences were only assigned to the superkingdom Viruses and the remaining third to the family Siphoviridae, followed by Myoviridae, Podoviridae, Tectiviridae, and Inoviridae. The metavirome showed a close relationship to the phage genomes that infect members of the classes Clostridia and Bacilli. Using publicly available biogas metagenomic data, a fragment recruitment approach showed the widespread distribution of the MAVGs studied in other biogas microbiomes. In particular, phage sequences from mesophilic microbiomes were highly similar to the phage sequences of this study. Accordingly, the virus particle enrichment approach and metavirome sequencing provided additional genome sequence information for novel virome members, thus expanding the current knowledge of viral genetic diversity in biogas reactors.
Application of a High-Throughput Targeted Sequence AmpliSeq Procedure to Assess the Presence and Variants of Virulence Genes in Salmonella
Authors: Gao Ruimin , Huang Hongsheng , Hamel Jérémie , Levesque Roger C. , Goodridge Lawrence D. , Ogunremi Dele
Published: February 2022

We have developed a targeted, amplicon-based next-generation sequencing method to detect and analyze 227 virulence genes (VG) of Salmonella (AmpliSeqSalm_227VG) for assessing the pathogenicity potential of Salmonella. The procedure was developed using 80 reference genomes representing 75 epidemiologically-relevant serovars associated with human salmonellosis. We applied the AmpliSeqSalm_227VG assay to (a) 35 previously characterized field strains of Salmonella consisting of serovars commonly incriminated in foodborne illnesses and (b) 34 Salmonella strains with undisclosed serological or virulence attributes, and were able to divide Salmonella VGs into two groups: core VGs and variable VGs. The commonest serovars causing foodborne illnesses such as Enteritidis, Typhimurium, Heidelberg and Newport had a high number of VGs (217–227). In contrast, serovars of subspecies not commonly associated with human illnesses, such as houtenae, arizonae and salame, tended to have fewer VGs (177–195). Variable VGs were not only infrequent but, when present, displayed considerable sequence variation: safC, sseL, sseD, sseE, ssaK and stdB showed the highest variation and were linked to strain pathogenicity. In a chicken infection model, VGs belonging to rfb and sse operons showed differences and were linked with pathogenicity. The high-throughput, targeted NGS-based AmpliSeqSalm_227VG procedure provided previously unknown information about variation in select virulence genes that can now be applied to a much larger population of Salmonella for evaluating pathogenicity of various serovars of Salmonella and for risk assessment of foodborne salmonellosis.
Diversity of Microbial Communities of Pinus sylvestris var. mongolica at Spatial Scale
Authors: Wang Dan-Dan , Zhao Wen , Reyila Mumin , Huang Kai-Chuan , Liu Shun , Cui Bao-Kai
Published: February 2022

Soil microorganisms play an indispensable role in the forest ecosystem. It is necessary to study the soil microorganisms in Pinus sylvestris var. mongolica, which is one of the afforestation species widely planted in the northern sandy region of China. We collected soil samples of P. sylvestris at large spatial scales and analyzed bacterial and fungal community composition differences using high-throughput sequencing techniques. The results showed that: (1) the richness index of different sandy lands was significantly different. The α-diversity of bacteria was the highest in Mu Us Sandy Land, and the α-diversity of fungi was the highest in Horqin Sandy Land. (2) The dominant phyla of bacteria were Actinobacteria, Proteobacteria, Chloroflexi and Acidobacteria, while the dominant phyla of fungi were Ascomycota and Basidiomycota. The relative abundance of dominant phyla was different. (3) Temperature and precipitation were the main driving factors of bacterial and fungal community change at large spatial scale. In addition, bacteria were also affected by total nitrogen, soil organic carbon and pH content; fungal community was affected by pH. The microorganisms showed obvious differences in geographical distribution, which could provide ideas for promoting sustainable management of P. sylvestris stand.
Calibrating Carbonization Temperatures of Wood Fragments Embedded within Pyroclastic Density Currents through Raman Spectroscopy
Authors: Schito Andrea , Pensa Alessandra , Romano Claudia , Corrado Sveva , Vona Alessandro , Trolese Matteo , Morgavi Daniele , Giordano Guido
Published: February 2022

The study of the structural order of charcoals embedded in pyroclastic density currents provides information on their emplacement temperature during volcanic eruptions. In the present work, a set of charcoals from three distinct pyroclastic density currents deposits whose temperatures have been previously estimated by charcoal reflectance analyses to lie between 250 °C and 550 °C, was studied by means of Raman spectroscopy. The analyses reveal a very disordered structural ordering of the charcoals, similar to kerogen matured under diagenetic conditions. Changes in Raman spectra at increasing temperatures reflect depolymerization and an increase of aromaticity and can be expressed by parameters derived from a simplified fitting method. Based on this approach, a second order polynomial regression with a high degree of correlation and a minimum error was derived to predict paleotemperatures of pyroclastic deposits. Our results show that Raman spectroscopy can provide a reliable and powerful tool for volcanological studies and volcanic hazard assessment given its advantage of minimum samples preparation, rapid acquisition processes and high precision.
An Experimental Study on the Effects of True Triaxial Loading and Unloading Stress Paths on the Mechanical Properties of Red Sandstone
Authors: Wang Shuai , Wang Lianguo , Tian Jiansheng , Fan Hao , Jiang Chongyang , Ding Ke
Published: February 2022

Loading and unloading stress paths play critical roles in investigating the deformation and failure of roadway excavation. In this study, tests under four different loading and unloading stress paths were conducted on red sandstone samples, with the aid of a self-developed true triaxial test system. Meanwhile, the deformation and failure characteristics of the samples were monitored during the tests. The following research conclusions were obtained: The octahedral shear stress is linearly correlated with the average effective stress, and the correlation coefficient R2 is 0.9825. The Mogi–Coulomb strength criterion is superior to the Drucker–Prager strength criterion in reflecting strength failure characteristics of red sandstone during loading and unloading. Shear failure tends to occur under uniaxial compression, whereas shear–tensile composite failure occurs under loading and unloading conditions. Compared with the true triaxial loading test, loading and unloading tests produce a larger strain in the unloading direction. Under loading and unloading stress paths, with the increase in intermediate principal stress (IPS), the strain in the direction of IPS gradually changes from expansion to compression, and the peak strength gradually increases. The state of IPS affects the failure strength of the sample and reflects the strengthening effect of IPS. This paper boasts a certain value and significance for research on the deformation and failure characteristics of sandstone in the actual in situ stress environment with triaxial dynamic changes.
Digitalization Solutions in the Mineral Processing Industry: The Case of GTK Mintec, Finland
Authors: Nad Alona , Jooshaki Mohammad , Tuominen Emilia , Michaux Simon , Kirpala Arno , Newcomb Johanna
Published: February 2022

The technologies used in mineral process engineering are evolving. The digital mineral processing solutions are based on advances in our ability to instrumentally measure phenomena at several stages of the beneficiation circuit, manage the data in real-time, and to analyze these data using machine learning to develop the next generation of process control. The main purpose of this study is to overview various digital solutions for mineral processing plants and characterization laboratories while emphasizing their utilization in the current state of the digitization process of the GTK Mintec. This study highlights the specialized digital technologies that are particularly relevant for mineral processing and beneficiation. The digital solutions studied in this article include digital twin, machine vision, information management system, sensors, smart equipment, machine learning techniques, process control system, robotic cell, and Internet of Things applied across the whole chain of studying materials from the mineralogical examinations through the bench-scale studies to the pilot test trials. The aim is to provide a clear view on the different aspects of digitizing mineral processing plants based upon the lessons learned from the development plans in GTK Mintec.
Excellent Electrocatalytic Hydrogen Evolution Reaction Performances of Partially Graphitized Activated-Carbon Nanobundles Derived from Biomass Human Hair Wastes
Authors: Sekar Sankar , Sim Dae Hyun , Lee Sejoon
Published: February 2022

Carbonaceous materials play a vital role as an appropriate catalyst for electrocatalytic hydrogen production. Aiming at realizing the highly efficient hydrogen evolution reaction (HER), the partially graphitized activated-carbon nanobundles were synthesized as a high-performance HER electrocatalyst by using biomass human hair ashes through the high-temperature KOH activation at two different temperatures of 600 and 700 °C. Due to the partial graphitization, the 700 °C KOH-activated partially graphitized activated-carbon nanobundles exhibited higher electrical conductivity as well as higher textural porosity than those of the amorphous activated-carbon nanobundles that had been prepared by the KOH activation at 600 °C. As a consequence, the 700 °C-activated partially graphitized activated-carbon nanobundles showed the extraordinarily high HER activity with the very low overpotential (≈16 mV at 10 mA/cm2 in 0.5 M H2SO4) and the small Tafel slope (≈51 mV/dec). These results suggest that the human hair-derived partially graphitized activated-carbon nanobundles can be effectively utilized as a high-performance HER electrocatalyst in future hydrogen-energy technology.
Preparation, Characterization, and Evaluation of Pyraclostrobin Nanocapsules by In Situ Polymerization
Authors: Huang Bingna , Chen Feifei , Shen Yue , An Changcheng , Li Ningjun , Jiang Jiajun , Wang Chong , Sun Changjiao , Zhao Xiang , Cui Bo , Zeng Zhanghua , Cui Haixin , Wang Yan
Published: February 2022

In this study, pyraclostrobin nanocapsules were prepared by in situ polymerization with urea–formaldehyde resin as a wall material. The effects of different emulsifiers, emulsifier concentrations, and solvents on the physicochemical properties of pyraclostrobin nanocapsules were investigated. Solvesso™ 100 was selected as the solvent, and Emulsifier 600# was used as the emulsifier, which accounted for 5% of the aqueous phase system, to prepare pyraclostrobin nanocapsules with excellent physical and chemical properties. The particle size, ζ potential, and morphology of the nanocapsules were characterized by a particle size analyzer and transmission electron microscope. The nanocapsules were analyzed by Fourier-transform infrared spectroscopy, and the loading content and sustained release properties of the nanocapsules were measured. The results show that the size of the prepared nanocapsules was 261.87 nm, and the polydispersity index (PDI) was 0.12, presenting a uniform spherical appearance. The loading content of the pyraclostrobin nanocapsules was 14.3%, and their cumulative release rate was 70.99% at 250 h, providing better efficacy and sustainability compared with the pyraclostrobin commercial formulation.
Efficient Hydrogen Evolution Reaction with Bulk and Nanostructured Mitrofanovite Pt3Te4
Authors: D’Olimpio Gianluca , Zhang Lixue , Kuo Chia-Nung , Farias Daniel , Ottaviano Luca , Lue Chin Shan , Fujii Jun , Vobornik Ivana , Agarwal Amit , Torelli Piero , Boukhvalov Danil W. , Politano Antonio
Published: February 2022

Here, we discuss the key features of electrocatalysis with mitrofanovite (Pt3Te4), a recently discovered mineral with superb performances in hydrogen evolution reaction. Mitrofanovite is a layered topological metal with spin-polarized topological surface states with potential applications for spintronics. However, mitrofanovite is also an exceptional platform for electrocatalysis, with costs of the electrodes suppressed by 47% owing to the partial replacement of Pt with Te. Remarkably, the Tafel slope in nanostructured mitrofanovite is just 33 mV/dec, while reduced mitrofanovite has the same Tafel slope (36 mV/dec) as state-of-the-art electrodes of pure Pt. Mitrofanovite also affords surface stability and robustness to CO poisoning. Accordingly, these findings pave the way for the advent of mitrofanovite for large-scale hydrogen production.
Effect of Alpha-S1-Casein Tryptic Hydrolysate and L-Theanine on Poor Sleep Quality: A Double Blind, Randomized Placebo-Controlled Crossover Trial
Authors: Thiagarajah Kokila , Chee Huei Phing , Sit Nam Weng
Published: February 2022

This randomized, placebo-controlled, crossover and double-blind study investigates the effects of RLX2™ containing alpha-s1-casein tryptic hydrolysate and L-theanine in working adults affected by poor sleep quality. The supplement or placebo was randomly and blindly assigned to 39 subjects for four weeks and the changes in the subjective sleep assessment via the Pittsburgh Sleep Quality Index (PSQI), heart rate, blood pressure, salivary cortisol by high-performance liquid chromatography method and alpha power of awake electroencephalogram (EEG) were studied. The data were analyzed in two ways, by crossover and crossover summed up. The latter depicted that RLX2™ improved PSQI total score, sleep latency, sleep duration, sleep habitual efficiency, daytime dysfunction, and increased total and frontal alpha power significantly (p < 0.05). The supplement prolonged the total sleeping time by 45 min in the supplement receiving group compared to the placebo group (p < 0.001). However, only sleep duration and sleep habitual efficiency showed a profound effect in both analyses (p < 0.05). In conclusion, being given its beneficial effects without notable adverse events, it would be advantageous to use these nutraceutical ingredients to promote better sleep quality. Further studies with a larger number participants are warranted to support these findings.
Substitution of Carbohydrates for Fats and Risk of Type 2 Diabetes among Korean Middle-Aged Adults: Findings from the Korean Genome and Epidemiology Study
Authors: Lee Hye-Ah , Park Hyesook
Published: February 2022

Using data from a 16 year follow-up cohort of the Korean Genome Epidemiology Study, this study assessed the effects of carbohydrate intake on incident diabetes, including replacement of fats or proteins with carbohydrates. In addition, this study evaluated modification effects based on 24 genetic variants associated with type 2 diabetes. For the daily intake of macronutrients, the energy-adjusted intake and percentage of total energy intake were calculated. The effects were assessed using a Cox proportional hazards model; results were presented as hazard ratios with 95% confidence intervals (CIs). Among the 7413 participants considered to be diabetes-free at baseline, 1193 individuals were considered to have incident diabetes. The risk of incident diabetes was found to be high at both extremes of carbohydrate intake, with the lowest risk at 78 E%. The replacement of 5 E% intake from fats with isocaloric carbohydrates showed an 11% increase in the risk of diabetes (95% CI: 1.01–1.21), which was significant in men, participants >50 years of age, and participants with a high educational level. Regarding gene–environment interactions, the relationship between carbohydrate intake and incident diabetes was not dependent on genetic variants. A nonlinear relationship was observed between carbohydrate intake and incident diabetes. The substitution of carbohydrates for fats was also associated with an increased risk of incident diabetes.
Terminology Matters: Advancing Science to Define an Optimal Pulse Intake
Authors: Mitchell Diane C. , Webster Alison , Garrison Becky
Published: February 2022

Confusion around the terms “legumes” and “pulses” has been a long-standing problem among consumers, health professionals, and researchers in the United States. The Food and Agricultural Organization defines pulses as legumes that are harvested solely as dry grain and include beans, peas, chickpeas, and lentils. For the first time ever, the 2020–2025 Dietary Guidelines for Americans recognized and used the terminology “pulses.” Correct terminology usage is important to build a solid research foundation that is specific to pulses, primarily because of their unique nutritional attributes that impact health differently than other legumes. Future widespread conformity and standardized use of a definition and categorization system around pulses versus legumes in research would allow for an improved interpretation of science and a better understanding of current research gaps. Clarity around these gaps could enhance and improve dietary recommendations, including the ability to refine our current understanding of the optimal daily or weekly intake of pulses at which health benefits are maximized.
A Comparison of Dietary Intake and Nutritional Status between Aged Care Residents Consuming Texture-Modified Diets with and without Oral Nutritional Supplements
Authors: Wu Xiaojing Sharon , Yousif Lina , Miles Anna , Braakhuis Andrea
Published: February 2022

Oral nutritional supplements (ONS) are high-energy and protein-rich nutrition drinks that are commonly prescribed to individuals with compromised nutritional status. Aged care residents requiring texture-modified diets are exposed to poor oral intake and malnutrition. This study aimed to investigate the dietary intake and nutritional status of residents consuming texture-modified diets with and without ONS. This multicentre cross-sectional study included 85 residents consuming texture-modified diets (86.0 ± 8.7 y; n = 46 requiring ONS and n = 39 without ONS). A one-day dietary record was completed using a validated visual plate waste estimation method. To determine the adequacy, nutrition intake was then calculated using FoodWorks (Xyris Ltd., Brisbane, Australia) and compared to the recommended dietary intake for Australia and New Zealand. The Mini-Nutritional Assessment Short Form was collected to assess nutritional status. Residents receiving ONS had significantly higher energy, protein, carbohydrates and fat intake than those who did not consume ONS (p < 0.05). No significant differences were found in saturated fat, fibre or sodium intake. With the administration of ONS, residents were able to meet their protein requirement but fell short of their energy and carbohydrates requirements. Both groups had inadequate fibre intake and a high saturated fat intake. A total of 48% of the residents were at risk of malnutrition and 38% were malnourished. Aged care residents requiring texture-modified diets are at high risk of malnutrition as a result of inadequate dietary intake. Administration of ONS may be an effective strategy to optimise nutrition intake.
Effectiveness of Nutritional Ingredients on Upper Gastrointestinal Conditions and Symptoms: A Narrative Review
Authors: Schulz Rebekah M. , Ahuja Nitin K. , Slavin Joanne L.
Published: February 2022

Nutritional ingredients, including various fibers, herbs, and botanicals, have been historically used for various ailments. Their enduring appeal is predicated on the desire both for more natural approaches to health and to mitigate potential side effects of more mainstream treatments. Their use in individuals experiencing upper gastrointestinal (GI) complaints is of particular interest in the scientific space as well as the consumer market but requires review to better understand their potential effectiveness. The aim of this paper is to review the published scientific literature on nutritional ingredients for the management of upper GI complaints. We selected nutritional ingredients on the basis of mentions within the published literature and familiarity with recurrent components of consumer products currently marketed. A predefined literature search was conducted in Embase, Medline, Derwent drug file, ToXfile, and PubMed databases with specific nutritional ingredients and search terms related to upper GI health along with a manual search for each ingredient. Of our literature search, 16 human clinical studies including nine ingredients met our inclusion criteria and were assessed in this review. Products of interest within these studies subsumed the categories of botanicals, including fiber and combinations, and non-botanical extracts. Although there are a few ingredients with robust scientific evidence, such as ginger and a combination of peppermint and caraway oil, there are others, such as melatonin and marine alginate, with moderate evidence, and still others with limited scientific substantiation, such as galactomannan, fenugreek, and zinc-l-carnosine. Importantly, the paucity of high-quality data for the majority of the ingredients analyzed herein suggests ample opportunity for further study. In particular, trials with appropriate controls examining dose–response using standardized extracts and testing for specific benefits would yield precise and effective data to aid those with upper GI symptoms and conditions.
Beneficial Effects of Spirulina Consumption on Brain Health
Authors: Trotta Teresa , Porro Chiara , Cianciulli Antonia , Panaro Maria Antonietta
Published: February 2022

Spirulina is a microscopic, filamentous cyanobacterium that grows in alkaline water bodies. It is extensively utilized as a nutraceutical food supplement all over the world due to its high levels of functional compounds, such as phycocyanins, phenols and polysaccharides, with anti-inflammatory, antioxidant, immunomodulating properties both in vivo and in vitro. Several scientific publications have suggested its positive effects in various pathologies such as cardiovascular diseases, hypercholesterolemia, hyperglycemia, obesity, hypertension, tumors and inflammatory diseases. Lately, different studies have demonstrated the neuroprotective role of Spirulina on the development of the neural system, senility and a number of pathological conditions, including neurological and neurodegenerative diseases. This review focuses on the role of Spirulina in the brain, highlighting how it exerts its beneficial anti-inflammatory and antioxidant effects, acting on glial cell activation, and in the prevention and/or progression of neurodegenerative diseases, in particular Parkinson’s disease, Alzheimer’s disease and Multiple Sclerosis; due to these properties, Spirulina could be considered a potential natural drug.
Who Is Running in the D-A-CH Countries? An Epidemiological Approach of 2455 Omnivorous, Vegetarian, and Vegan Recreational Runners—Results from the NURMI Study (Step 1)
Authors: Wirnitzer Katharina , Motevalli Mohamad , Tanous Derrick , Wirnitzer Gerold , Leitzmann Claus , Pichler Renato , Rosemann Thomas , Knechtle Beat
Published: February 2022

Accompanied by the growing popularity of distance running, the prevalence of vegan and vegetarian diets in endurance runners has increased across the globe and especially in German-speaking (D-A-CH: Germany, Austria, Switzerland) countries. The present study aimed to investigate and compare the epidemiological characteristics associated with diet types and running behaviors of recreational endurance runners. From a total number of 7422 runners who started to fill in the online survey, 3835 runners completed the questionnaire. After data clearance, 2455 distance runners (mean age: 37 years; 56% females, 44% males) were selected as the final sample and classified as 1162 omnivores (47.4%), 529 vegetarians (21.5%), and 764 vegans (31.1%). Sociodemographic information and general characteristics in training and competition were evaluated using a questionnaire-based approach. A significant association was found between diet type and race distance (p < 0.001). In females, vegan ultra-marathoners and omnivorous half-marathoners had better individual running records among dietary groups. Sex differences in running performance had a minimizing trend with increasing race distance. Most runners reported independent race preparation (90%) over less than four months (73%). From an epidemiological viewpoint, the present findings suggest a central role of plant-based diets in running performance and behaviors among active distance runners in D-A-CH countries and that vegetarian and vegan diets are compatible with competitive running.
Temporal Muscle and Stroke—A Narrative Review on Current Meaning and Clinical Applications of Temporal Muscle Thickness, Area, and Volume
Authors: Katsuki Masahito , Kakizawa Yukinari , Nishikawa Akihiro , Yamamoto Yasunaga , Uchiyama Toshiya , Agata Masahiro , Wada Naomichi , Kawamura Shin , Koh Akihito
Published: February 2022

Background: Evaluating muscle mass and function among stroke patients is important. However, evaluating muscle volume and function is not easy due to the disturbances of consciousness and paresis. Temporal muscle thickness (TMT) has been introduced as a novel surrogate marker for muscle mass, function, and nutritional status. We herein performed a narrative literature review on temporal muscle and stroke to understand the current meaning of TMT in clinical stroke practice. Methods: The search was performed in PubMed, last updated in October 2021. Reports on temporal muscle morphomics and stroke-related diseases or clinical entities were collected. Results: Four studies reported on TMT and subarachnoid hemorrhage, two studies on intracerebral hemorrhage, two studies on ischemic stroke, two studies on standard TMT values, and two studies on nutritional status. TMT was reported as a prognostic factor for several diseases, a surrogate marker for skeletal muscle mass, and an indicator of nutritional status. Computed tomography, magnetic resonance imaging, and ultrasonography were used to measure TMT. Conclusions: TMT is gradually being used as a prognostic factor for stroke or a surrogate marker for skeletal muscle mass and nutritional status. The establishment of standard methods to measure TMT and large prospective studies to further investigate the relationship between TMT and diseases are needed.
De Novo Synthesis of Bacteriochlorins Bearing Four Trideuteriomethyl Groups
Authors: Jing Haoyu , Tang Qun , Bocian David F. , Lindsey Jonathan S.
Published: February 2022

Site-specific introduction of isotopes in tetrapyrrole macrocycles provides the foundation for probing physicochemical features germane to photosynthetic energy-transduction processes, but has chiefly been done with porphyrins rather than the more biologically relevant hydroporphyrin analogues of native photosynthetic pigments. A prior study incorporated pairwise 13C or 15N atoms in the skeleton of a bacteriochlorin containing a gem-dimethyl group in each pyrroline ring. Here, a complementary effort is reported that installs deuterium atoms in substituents at the perimeter of a bacteriochlorin. Thus, perdeuteriated 3-methyl-2,4-pentanedione was converted in an 8-step synthesis via the intermediacy of tert-butyl 5-formyl-3,4-bis(trideuteriomethyl)pyrrole-2-carboxylate to the 2,3,12,13-tetrakis(trideuteriomethyl)-8,8,18,18-tetramethylbacteriochlorin (BC-2). The fidelity of isotope substitution was maintained throughout the synthesis. Resonance Raman spectroscopy of the copper chelate (CuBC-2) revealed that addition of the four β-pyrrolic substituents alone is not sufficient to account for the vibronic complexity observed for the copper chelate of bacteriochlorophyll a (CuBChl a). The increased vibronic activity exhibited by the natural pigments and CuBChl a must arise from the increased structural complexity of the macrocycle.