bluuebunny/crossref_metadata_2025 huggingface dataset:
Hemostatic Status of Neonates with Perinatal Hypoxia, Studied via NATEM in Cord Blood Samples
Authors: Tsaousi Marina , Sokou Rozeta , Pouliakis Abraham , Politou Marianna , Iacovidou Nicoletta , Boutsikou Theodora , Sulaj Alma , Karapati Eleni , Tsantes Andreas G. , Tsantes Argirios E. , Valsami Serena , Iliodromiti Zoi
Published: July 2024

Background: Perinatal hypoxia may result in coagulation dysfunction. Diminished blood flow or oxygen to the fetus/neonate during the perinatal period can cause bone marrow and liver function impairment, leading to thrombocytopenia, impaired synthesis of clotting and fibrinolytic factors, and increased destruction of platelets in the small blood vessels. The goal of the present study was to evaluate the hemostatic status of newborns with perinatal hypoxia via the non-activated thromboelastometry (NATEM) assay in cord blood samples. Methods: 134 hypoxic neonates born in our maternity unit over a 1.5-year period were enrolled in this observational cohort study, and 189 healthy neonates served as the control group. Participation in the study was voluntary and parents signed informed consent prior to recruitment. Demographic and clinical data were recorded on admission, and the NATEM method was performed on cord blood samples. The following NATEM values were evaluated: clotting time (CT), alpha angle (α-angle), clot formation time (CFT), clot amplitude at 5 and 10 min. (A5, A10), maximum clot firmness (MCF), clot lysis index at 60 min. after CT (LI60), and maximum clot elasticity (MCE). Statistical analysis was conducted utilizing the SAS for Windows 9.4 software platform. Results: Neonates with perinatal hypoxia exhibited decreased fibrinolytic potential in comparison to healthy neonates, as indicated by increased LI60, and this difference was statistically significant (LΙ60: 94 (92–96) Vs 93 (91–95), p value = 0.0001). There were no statistically significant differences noted among the remaining NATEM variables. Conclusion: Our findings indicate decreased fibrinolytic potential in hypoxic neonates in comparison to healthy neonates, suggesting that NATEM could serve as an effective tool for promptly identifying hemostasis dysfunction in this group of neonates.
The Similarity Degree of the Anthropometric and Body Composition Variables of Brazilian and Mozambican Schoolchildren: A New Approach Using the Smoothed Jaccard Index Surface
Authors: Pacheco André Luiz de Góes , Bocanegra Willian Motta , Ferreira Élida Karina de Lira , dos Santos Rayssa Temudo , Simões Patrícia Miller , Malinga Eulálio , Guiliche Euclides da Conceição , Isaías Arsénio Fernando José , Nobre Isabele Góes , Silva João Henrique da Costa , Silva Wylla Tatiana Ferreira e , Padrón-Hernandez Eduardo , Pirola Luciano , Henrique Rafael dos Santos , Leandro Carol Góis
Published: July 2024

Background/Objectives: Brazil and Mozambique face similar socioeconomic challenges, including common indicators of undernutrition and overnutrition among children. This study evaluated the similarity degree of the anthropometric and body composition variables of Brazilian and Mozambican children by using the Jaccard index. Methods: A total of 1831 children of both genders aged 7–10 years from three Brazilian cities (Recife, Vitoria de Santo Antao, and Lagoa do Carro) and three Mozambican cities (Maputo, Boane, and Inhambane) participated in this study. Anthropometric (height, body mass, and waist circumference) and body composition (body fat percentage [%BF], lean mass, and fat mass) variables were measured and the Smoothed Jaccard Index Surface (SJIS) was used to evaluate the similarity degree. Results: Brazilian children were taller and heavier and had a higher %BF and fat mass than Mozambican children. Children living in urban areas were taller than those living in rural zones in both countries. Brazilian and Mozambican children showed high similarity only between %BF and lean mass. Children from Recife and Maputo had high similarities among waist circumference, body mass, fat mass, height, and %BF. Finally, a high SJIS degree was observed among height and %BF for schoolchildren from rural and urban zones. Conclusion: Brazilian and Mozambican children exhibit differences in growth characteristics but a high degree of similarity when children from rural and urban zones are compared.
Design of Tool Wear Monitoring System in Bone Material Drilling Process
Authors: Liu Lijia , Kang Wenjie , Wang Yiwen , Zeng Lingchen
Published: July 2024

Biological bone materials, complex and anisotropic, require precise machining in surgeries. Bone drilling, a key technique, is susceptible to increased friction from tool wear, leading to excessive forces and high temperatures that can damage bone and surrounding tissues, affecting recovery. This study develops a monitoring platform to assess tool wear during bone drilling, employing an experimental setup that gathers triaxial force and vibration data. A recognition model using a bidirectional long short-term memory network (BI-LSTM) with a multi-head attention mechanism identified wear levels. This model, termed ABI-LSTM, was optimized and benchmarked against SVR, RNN, and CNN models. The results from implementing the ABI-LSTM-based monitoring system demonstrated its efficacy in detecting tool wear, thereby potentially reducing surgical risks such as osteonecrosis and drill breakage, and enhancing surgical outcomes.
Anodizing of AA2024 Aluminum–Copper Alloy in Citric-Sulfuric Acid Solution: Effect of Current Density on Corrosion Resistance
Authors: Cabral-Miramontes Jose , Cabral-Miramontes Noe , Nieves-Mendoza Demetrio , Lara-Banda Maria , Maldonado-Bandala Erick , Olguín-Coca Javier , Lopez-Leon Luis Daimir , Estupiñan-Lopez Francisco , Calderon F. Almeraya , Gaona Tiburcio Citlalli
Published: July 2024

Al–Cu alloys are widely used as a structural material in the manufacture of commercial aircraft due to their high mechanical properties such as hardness, strength, low density, and tolerance to fatigue damage and corrosion. One of the main problems of these Al–Cu alloy systems is their low corrosion resistance. The purpose of this study is to analyze the influence of anodizing parameters on aluminum–copper alloy (AA 2024) using a bath of citric-sulfuric acid with different anodizing current densities on the thickness, microhardness, and corrosion resistance of the anodized layer. Hard anodizing is performed on AA 2024 Al–Cu alloy in mixtures of solutions composed of citric and sulfuric acid at different concentrations for 60 min and using current densities (i) of 0.03, 0.045, and 0.06 A/cm2. Scanning electron microscopy (SEM) was used to analyze the surface morphology and thickness of the anodized layer. The mechanical properties of the hard anodized material are evaluated using the Vickers hardness test. The electrochemical techniques use cyclic potentiodynamic polarization curves (CPPC) according to ASTM-G6 and electrochemical impedance spectroscopy (EIS) according to ASTM-G61 and ASTM-G106, respectively, in the electrolyte of NaCl at 3.5 wt. % as a simulation of the marine atmosphere. The results indicate that corrosion resistance anodizing in citric-sulfuric acid solutions with a current density of 0.06 A/cm2 is the best with a corrosion current density (jcorr) of 1.29 × 10−8 A/cm2. It is possible to produce hard anodizing with citric and sulfuric acid solutions that exhibit mechanical properties and corrosion resistance similar or superior to conventional sulfuric acid anodizing.
Anti-Corrosion SiOx-Doped DLC Coating for Raster Steel Linear Scales
Authors: Lazauskas Algirdas , Grigaliūnas Viktoras , Jucius Dalius , Meškinis Šarūnas , Andrulevičius Mindaugas , Guobienė Asta , Vasiliauskas Andrius , Kasparaitis Albinas
Published: July 2024

In this study, we investigated the efficacy of SiOx-doped diamond-like carbon (DLC) films for enhancing the corrosion resistance of raster steel linear scales. The research work highlights the significant role of DLC film materials in enhancing corrosion resistance, making them a promising solution for various industrial applications. The Raman spectroscopy analysis of SiOx-doped DLC films, synthesized via a direct ion beam technique with HMDSO vapor, revealed prominent D and G bands characteristic of amorphous carbon materials, with a high degree of disorder indicated by an ID/IG ratio of 1.85. X-ray diffraction patterns confirmed the amorphous nature of the SiOx-doped DLC films and the minimal impact of the DLC deposition process on the underlying crystalline structure of steel. UV–Vis-NIR reflectance spectra of SiOx-doped DLC on stainless steel demonstrated improvements in the blue wavelength region compared to stainless steel with ripples alone, which is beneficial for applications utilizing blue light. Corrosion tests, including immersion in a 5% salt solution and salt spray testing, showed that SiOx-doped DLC-coated stainless steel exhibited superior corrosion resistance compared to uncoated steel, with no significant signs of corrosion observed after extended exposure. These findings underscore the potential of SiOx-doped DLC coatings to provide long-term corrosion protection and maintain the structural integrity and surface quality of steel components in harsh environments.
Performance and Accelerated Ageing of an Industrial Hydraulic Lime Mortar Applied on Different Substrates
Authors: Travincas Rafael , Silveira Dora , Bellei Poliana , Gouveia João , Matias Gina , Torres Isabel , Flores-Colen Inês
Published: July 2024

Mortar that is typically employed for interior or exterior coatings can be characterised using laboratory-prepared specimens according to specific test standards; however, its performance undergoes changes following application on substrates. When selecting mortar, it is vital to anticipate its in-service behaviour after its application on substrates to make the most informed choice. Most of the research work carried out to date analyses the characteristics of mortar in laboratory specimens. Some studies analyse these characteristics after its application to support, but very few exist that compare both behaviours. With this objective in mind, this research determined the properties of mortar when cured within laboratory moulds and assessed the behaviour of the same mortar after application on diverse substrate types. This study specifically evaluated the behaviour of a pre-dosed hydraulic lime mortar when applied on concrete blocks, lightweight concrete blocks, concrete slabs, hollow ceramic bricks, and solid ceramic bricks. Later, this behaviour was compared to the same type of mortar hardened in laboratory moulds and the same type of mortar applied on substrates and subjected to accelerated ageing. Moreover, data from previous experimental work were used to compare the behaviour of the pre-dosed hydraulic lime mortar with that of pre-dosed cement mortar when applied on similar substrates. The research drew upon a comprehensive characterisation of the physical and mechanical parameters of mortar, revealing that the performance of these types of mortar undergoes significant changes after application on substrates under in-service conditions, mainly when applied on more porous substrates. It was concluded that the application of mortar to substrates increased bulk density, decreased open porosity, enhanced compressive strength, and resulted in faster capillary absorption. For mortars subjected to accelerated ageing, a notable reduction in water vapour permeability was observed, which was attributed to changes in the pore profile.
Effect of Cooling Method on Microstructure and Microhardness of CuCrFeMnNi High-Entropy Alloy
Authors: Zhou Yajun , Zhao Ruifeng , Geng Hechuan , Ren Bo , Liu Zhongxia , Liu Jianxiu , Jiang Aiyun , Zhang Baofeng
Published: July 2024

This study investigated four cooling methods for CuCrFeMnNi high-entropy alloy, namely, furnace cooling, air cooling, oil cooling, and water cooling (designated as FC, AC, OC, and WC, respectively), following a 12 h treatment at 800 °C. Results indicate that all four cooled alloys consisted of two FCC solid-solution phases (FCC1 and FCC2) and ρ phases. However, the FC alloy primarily contained FCC2 as the main phase and FCC1 as the secondary phase. The other three cooling methods yielded alloys with FCC2 as the primary phase and FCC1 as the secondary phase. With an increase in cooling rate, the content of the FCC1 phase gradually increased, that of the ρ phase initially decreased and then increased, and that of the FCC2 phase gradually decreased. The microstructure of the CuCrFeMnNi high-entropy alloy under the four cooling methods consisted of gray-black dendrites rich in Cr-Fe and white dendrites rich in Cu. Black ρ-phase particles predominated the dendrite region. As the cooling rate increased, the white interdendritic regions shrank, and the gray-black interdendritic regions expanded. The FC alloy exhibited the lowest microhardness at approximately 202.6 HV. As the cooling rate increased, the microhardness of the alloy progressively increased. The microhardness of the WC alloy was the highest, at approximately 355 HV. The strengthening mechanisms for all the alloys were primarily solid-solution strengthening and second-phase precipitation strengthening.
A Review of Capacity Fade Mechanism and Promotion Strategies for Lithium Iron Phosphate Batteries
Authors: Hu Chen , Geng Mengmeng , Yang Haomiao , Fan Maosong , Sun Zhaoqin , Yu Ran , Wei Bin
Published: July 2024

Commercialized lithium iron phosphate (LiFePO4) batteries have become mainstream energy storage batteries due to their incomparable advantages in safety, stability, and low cost. However, LiFePO4 (LFP) batteries still have the problems of capacity decline, poor low-temperature performance, etc. The problems are mainly caused by the following reasons: (1) the irreversible phase transition of LiFePO4; (2) the formation of the cathode–electrolyte interface (CEI) layer; (3) the dissolution of the iron elements; (4) the oxidative decomposition of the electrolyte; (5) the repeated growth and thickening of the solid–electrolyte interface (SEI) film on the anode electrode; (6) the structural deterioration of graphite anodes; (7) the growth of lithium dendrites. In order to eliminate the problems, methods such as the modification, doping, and coating of cathode materials, electrolyte design, and anode coating have been studied to effectively improve the electrochemical performance of LFP batteries. This review briefly describes the working principle of the LFP battery, the crystal structure of the LFP cathode material, and its electrochemical performance as a cathode. The performance degradation mechanism of LFP batteries is summarized in three aspects—cathode material, anode material, and electrolyte—and the research status of LFP material modification and electrolyte design is emphatically discussed. Finally, the challenges and future development of LFP batteries are prospected.
Correction: Kaushik, P.; Kumar, S. Transcriptome Analysis of Bael (Aegle marmelos (L.) Corr.) a Member of Family Rutaceae. Forests 2018, 9, 450
Authors: Kaushik Prashant , Kumar Shashi
Published: July 2024

The (Forests) Editorial Office wishes to make the following changes to the author’s paper [...]
The Bacharach Method: A Low-Cost Tool for Small-Scale Combustion Units’ Flue Gas Quality Control
Authors: Ryšavý Jiří , Yan Wei-Mon , Sangeetha Thangavel , Kuo Jenn-Kun , Wang Cheng-Chi , Hopan František , Gouveia Maria , Henriques Carla Oliveira , Kuboňová Lenka , Ochodek Tadeáš
Published: July 2024

Although current EU regulations, such as EU Directive 2015/1189 on the eco-design of solid fuel boilers and Directive 2015/1188, in accordance with the Machinery Directive 2006/42/EC, require manufacturers to meet specific emission requirements for CE marking, the routine and regular onsite testing of household heating appliances is still not mandatory in many EU countries. This research endeavour addressed this gap by evaluating the effectiveness of the Bacharach method as a rapid and cost-effective tool for assessing flue gas quality, particularly in terms of particulate matter mass concentration. This study also compared the results of the Bacharach method with those obtained from two commercially available portable analysers. The research outcomes demonstrate that the Bacharach method, in combination with an innovative evaluation approach, offers a viable solution, enabling the swift and economical assessment of flue gas quality with the primary objective of determining the boiler class according to the limits specified by standard EN 303-5 under operating conditions. The modified Bacharach method for measuring TSP in solid fuel-fired boilers provides qualitatively similar results to the commercially used SM500 and STM225 instruments. The modified Bacharach methodology was primarily developed for comparison to the boiler class 3 limit (i.e., 125 and 150 mg/m3). The study revealed that the modified Bacharach method, when applied to biomass-based boilers, exhibited higher accuracies in the case of classification into classes 3 and 4, whereas fossil fuel-based boilers demonstrated higher accuracy in the case of class 5 limits.
Population Genetics of the Endemic Hemiculterella wui (Wang, 1935) in the Poyang Lake Basin (China)
Authors: Ma Qin , Li Mingzheng , Liu Huanzhang
Published: July 2024

The Yangtze River floodplain is an area with an extremely rich diversity of fish species. Poyang Lake, as an important part of this river–floodplain system, is a crucial habitat for the survival of fish. However, prolonged human activities, such as environmental pollution and hydroelectric development, have degraded the habitat in the Poyang Lake Basin, posing threats to fish populations. Understanding genetic diversity is crucial for maintaining fish populations and understanding their dynamics. The genetic diversity of Hemiculterella wui, an economically endemic species in China of subfamily Cultrinae (Cypriniformes: Cyprinidae), has been understudied. This study investigated the genetic diversity and structure of H. wui populations from the Ganjiang, Xinjiang, Fuhe, and Raohe rivers in the Poyang Lake Basin using mitochondrial Cytb gene analysis. Results showed high haplotype diversity but low nucleotide diversity in H. wui’s Cytb sequences. Analysis of molecular variance (AMOVA) showed no significant geographic genetic structure among populations. Haplotype network analysis revealed no clear geographical clustering. Neutrality tests and haplotype nucleotide mismatch distribution indicated that all populations had experienced expansion events. These findings suggest that H. wui in Poyang Lake does not show a distinct geographic structure. However, it is still necessary to monitor the genetic characteristics of H. wui to maintain the genetic diversity of fishes in Poyang Basin, considering the threat of habitat loss and fragmentation to the population.
Influence of Local Thermodynamic Non-Equilibrium to Photothermally Induced Acoustic Response of Complex Systems
Authors: Galovic Slobodanka , Djordjevic Aleksa I. , Kovacevic Bojan Z. , Djordjevic Katarina Lj. , Chevizovich Dalibor
Published: July 2024

In this paper, the time-resolved model of the photoacoustic signal for samples with a complex inner structure is derived including local non-equilibrium of structural elements with multiple degrees of freedom, i.e., structural entropy of the system. The local non-equilibrium is taken into account through the fractional operator. By analyzing the model for two types of time-dependent excitation, a very short pulse and a very long pulse, it is shown that the rates of non-equilibrium relaxations in complex samples can be measured by applying the derived model and time-domain measurements. Limitations of the model and further directions of its development are discussed.
Mitigative Effect of Graphene Oxide Nanoparticles in Maintaining Gut–Liver Homeostasis against Alcohol Injury
Authors: Aghara Hiral , Chadha Prashsti , Mandal Palash
Published: July 2024

Alcoholic liver disease (ALD) develops when the immunotolerant environment of the liver is compromised due to excessive alcohol consumption. ALD progression involves variations in the expressions of multiple genes, resulting in liver inflammation and the development of a leaky gut. It is still unclear which molecular mechanism is involved in ALD progression, and due to that, there are currently no FDA-approved drugs available for its treatment. In this study, the protective effects of graphene oxide (GO) nanoparticles were investigated against ethanol-induced damage in the gut–liver axis in in vitro. GO was synthesized using a modified Hummer’s method, and characterization was performed. Given the general concerns regarding nanoparticle toxicity, assessments of cell viability, lipid accumulation, DNA damage, cell death, and the generation of reactive oxygen species (ROS) were conducted using various techniques. Furthermore, the gene expressions of pro- and anti-inflammatory cytokines were determined using RT-qPCR. The findings reveal that GO promoted cell viability even against ethanol treatment. Additionally, lipid accumulation significantly decreased when cells were treated with GO alongside ethanol compared to ethanol treatment alone, with similar trends observed for other assays. A gene expression analysis indicated that GO treatment reduced the expression of proinflammatory cytokines while enhancing the expression of antioxidant genes. Moreover, GO treatment led to improvements in gut integrity and a reduction in proinflammatory cytokines in colon cells damaged by ethanol. These findings suggest that GO holds promise as a drug carrier, exhibiting no observed toxic effects. By shedding light on the protective effects of GO against ethanol-induced damage, this study contributes to the burgeoning field of nanoparticle-mediated therapy for ALD.
“What Keeps Me in School”: Oregon BIPOC Learners Voice Support That Makes Higher Education Possible
Authors: Hunte Roberta Suzette , Mosier-Puentes Miranda , Mehrotra Gita , Skuratowicz Eva
Published: July 2024

A growing number of college students are nontraditional learners (age 21–65) who are people of color. These students face unique challenges in a higher education system increasingly shaped by neoliberalism and the ongoing context of institutionalized racism. In Oregon, policymakers have established ambitious goals to address racial disparities in educational attainment. In this study, focus groups and interviews were conducted with 111 Black, Indigenous, and People of Color (BIPOC) adult learners in Oregon to better understand their perspectives and experiences in regard to educational and career pathways. Participants included currently enrolled students, adults who had enrolled and left, and adults who had never enrolled in post-secondary education. Thematic analysis focused on support that facilitates educational access and persistence for these learners. Consistent with the existing literature, our findings revealed that support fell into three broad categories: economic, social/cultural, and institutional support. Recommendations focus on utilizing targeted universalism as a strategy for supporting non-traditional students of color to access and complete college through the expansion of economic support for students, shoring up relevant academic and career resources, and building more meaningful partnerships between higher education and communities of color. Limitations and directions for future research are also discussed.
The Sámi Pathfinders: Addressing the Knowledge Gap in Norwegian Mainstream Education
Authors: Walsh-Knarvik Kimble
Published: July 2024

For at least two decades, lack of knowledge about the Sámi in Norway has been recognised as a reason for the perpetuation of stereotypes and discriminatory acts and hate speech towards them. Education about the Sámi, their lifeways, culture and rights is posited as a means of closing this gap, with the intention of influencing the majority Norwegian society’s attitudes towards the Sámi. The relatively new Norwegian curriculum (LK20) reflects this understanding. It requires teachers at every level of the educational system to include Sámi perspectives and themes in all subjects. This paper looks at how Indigenous Education is included in mainstream schools in Norway. It asks, if Indigenous Education can provide a counterbalance to existing stereotypes and discrimination of the Sámi People, then what kind of knowledge is sufficient to this end? To explore this, I specifically consider the efforts of the Sámi Pathfinders—a group of young Sámi adults (18–25 years) who visit and provide lectures about Sámi history, language and culture for Norwegian high school pupils. Through semi-structured interviews with five Pathfinders, I explored what kind of Indigenous Education they provide, how the Pathfinders interpret their role in relation to combatting stereotypes and discrimination, and their perception of the impact they have. Through reflexive thematic analysis, this study confirmed that there is a lack of knowledge about the Sámi in mainstream education. It also shows that most teachers did not prepare their pupils for the Pathfinders’ visit. Although the Pathfinders’ visit arguably improved pupils’ and teachers’ knowledge about the Sámi, this research suggests that how and how often knowledge is presented matters. It also suggests that who presents knowledge is a factor. Indigenous knowledge that is coupled with contact that is sufficiently close, positive and frequent has greater potential in altering discriminatory tendencies towards the Sámi.
EDA Missense Variant in a Cat with X-Linked Hypohidrotic Ectodermal Dysplasia
Authors: Rietmann Stefan J. , Cochet-Faivre Noëlle , Dropsy Helene , Jagannathan Vidhya , Chevallier Lucie , Leeb Tosso
Published: July 2024

Hypohidrotic ectodermal dysplasia is a developmental defect characterized by sparse or absent hair, missing or malformed teeth and defects in eccrine glands. Loss-of-function variants in the X-chromosomal EDA gene have been reported to cause hypohidrotic ectodermal dysplasia in humans, mice, dogs and cattle. We investigated a male cat exhibiting diffuse truncal alopecia with a completely absent undercoat. The cat lacked several teeth, and the remaining teeth had an abnormal conical shape. Whole-genome sequencing revealed a hemizygous missense variant in the EDA gene, XM_011291781.3:c.1042G>A or XP_011290083.1:p.(Ala348Thr). The predicted amino acid exchange is located in the C-terminal TNF signaling domain of the encoded ectodysplasin. The corresponding missense variant in the human EDA gene, p.Ala349Thr, has been reported as a recurring pathogenic variant in several human patients with X-linked hypohidrotic ectodermal dysplasia. The identified feline variant therefore represents the likely cause of the hypohidrotic ectodermal dysplasia in the investigated cat, and the genetic investigation confirmed the suspected clinical diagnosis. This is the first report of an EDA-related hypohidrotic ectodermal dysplasia in cats.
New Observations of the Effects of the Cytoplasm of Aegilops kotschyi Boiss. in Bread Wheat Triticum aestivum L.
Authors: Fan Chaolan , Melonek Joanna , Lukaszewski Adam J.
Published: July 2024

The cytoplasm of Aegilops kotschyi is known for the induction of male sterility and haploidy in wheat. Both systems originally appeared rather simple, but manipulation of the standard chromosome constitution of the nuclear genome revealed additional interactions. This study shows that while there is little or no allelic variation at the main fertility restorer locus Rfmulti on chromosome arm 1BS, additional genes may also be involved in the nuclear–mitochondrial genome interactions, affecting not only male fertility but also the growth rate, from pollen competition for fertilization and early endosperm divisions all the way to seed size and plant maturity. Some of these effects appear to be of a sporophytic nature; others are gametophytic. Induction of parthenogenesis by a rye inducer in conjunction with the Ae. kotschyi cytoplasm is well known. However, here we show that the cytoplasmic-nuclear interactions affect all aspects of double fertilization: producing maternal haploids from unfertilized eggs, diploids from fertilized eggs or synergids, embryo-less kernels, and fertilized eggs without fertilization of the double nucleus in the embryo sack. It is unclear how frequent the inducers of parthenogenesis are, as variation, if any, is obscured by suppressors present in the wheat genome. Genetic dissection of a single wheat accession revealed five distinct loci affecting the rate of maternal haploid production: four acting as suppressors and one as an enhancer. Only when the suppressing haplotypes are confirmed may it be possible to the identify genetic variation of haploidy inducers, map their position(s), and determine their nature and the mode of action.
Lynch Syndrome and Thyroid Nodules: A Single Center Experience
Authors: Spinelli Irene , Moffa Simona , Fianchi Francesca , Mezza Teresa , Cinti Francesca , Di Giuseppe Gianfranco , Marmo Clelia , Ianiro Gianluca , Ponziani Francesca Romana , Tortora Annalisa , Riccioni Maria Elena , Giaccari Andrea , Gasbarrini Antonio
Published: July 2024

Background: Lynch syndrome (LS) is a genetic disease with increased risk of colorectal cancer and other malignancies. There are few reported cases of thyroid cancer in LS patients. The aim of this study is to investigate the presence of thyroid nodules in LS patients and to explore their association with the genetic features of the disease. Methods: A retrospective and descriptive analysis was conducted to include all LS patients followed at the CEMAD (Centro Malattie Apparato Digerente) of Fondazione Policlinico Universitario A. Gemelli IRCCS. The characteristics of LS disease, gene mutations, and previous history of thyroid disease were evaluated. Majority of patients underwent thyroid ultrasound (US), and nodule cytology was performed when needed. Results: Of a total of 139 patients with LS, 110 patients were included in the study. A total of 103 patients (74%) underwent thyroid ultrasound examinations, and 7 patients (5%) had a previous history of thyroid disease (cancer or multinodular goiter). The mean age was 51.9 years. Thyroid nodules were found in 62 patients (60%) who underwent US, and 9 of them (14%) had suspicious features of malignancy, inducing a fine-needle aspiration biopsy. A cytologic analysis classified 7 of 9 cases (78%) as TIR2 and 2 (22%) as TIR3a. Between patients with nodular thyroid disease (single nodule, multinodular goiter, and cancer), most of them (25 patients, 36% of total) were carriers of the MSH6 mutation, while 22 (32%), 17 (24%), and 5 (7%) had MSH2, MLH1, and PMS2 mutations, respectively. Conclusions: A high prevalence of thyroid nodules was found in patients with LS, especially in MSH6-carrying patients. Performing at least one thyroid ultrasound examination is suggested for the detection of nodular thyroid disease in LS patients. Systematic investigations are needed to estimate their prevalence, features, and risk of malignant transformation.
Intermolecular Gene Conversion for the Equalization of Genome Copies in the Polyploid Haloarchaeon Haloferax volcanii: Identification of Important Proteins
Authors: Özer Hanna , Wasser Daniel , Sandner Lara , Soppa Jörg
Published: July 2024

The model haloarchaeon Haloferax volcanii is polyploid with about 20 copies of its major chromosome. Recently it has been described that highly efficient intermolecular gene conversion operates in H. volcanii to equalize the chromosomal copies. In the current study, 24 genes were selected that encode proteins with orthologs involved in gene conversion or homologous recombination in archaea, bacteria, or eukaryotes. Single gene deletion strains of 22 genes and a control gene were constructed in two parent strains for a gene conversion assay; only radA and radB were shown to be essential. Protoplast fusions were used to generate strains that were heterozygous for the gene HVO_2528, encoding an enzyme for carotinoid biosynthesis. It was revealed that a lack of six of the proteins did not influence the efficiency of gene conversion, while sixteen mutants had severe gene conversion defects. Notably, lack of paralogous proteins of gene families had very different effects, e.g., mutant Δrad25b had no phenotype, while mutants Δrad25a, Δrad25c, and Δrad25d were highly compromised. Generation of a quadruple rad25 and a triple sph deletion strain also indicated that the paralogs have different functions, in contrast to sph2 and sph4, which cannot be deleted simultaneously. There was no correlation between the severity of the phenotypes and the respective transcript levels under non-stressed conditions, indicating that gene expression has to be induced at the onset of gene conversion. Phylogenetic trees of the protein families Rad3/25, MutL/S, and Sph/SMC/Rad50 were generated to unravel the history of the paralogous proteins of H. volcanii. Taken together, unselected intermolecular gene conversion in H. volcanii involves at least 16 different proteins, the molecular roles of which can be studied in detail in future projects.
Objective Assessments of Smoking and Drinking Outperform Clinical Phenotypes in Predicting Variance in Epigenetic Aging
Authors: Philibert Robert , Lei Man-Kit , Ong Mei Ling , Beach Steven R. H.
Published: July 2024

The reliability of the associations of the acceleration of epigenetic aging (EA) indices with clinical phenotypes other than for smoking and drinking is poorly understood. Furthermore, the majority of clinical phenotyping studies have been conducted using data from subjects of European ancestry. In order to address these limitations, we conducted clinical, physiologic, and epigenetic assessments of a cohort of 278 middle-aged African American adults and analyzed the associations with the recently described principal-components-trained version of GrimAge (i.e., PC-GrimAge) and with the DunedinPACE (PACE) index using regression analyses. We found that 74% of PC-GrimAge accelerated aging could be predicted by a simple baseline model consisting of age, sex, and methylation-sensitive digital PCR (MSdPCR) assessments of smoking and drinking. The addition of other serological, demographic, and medical history variables or PACE values did not meaningfully improve the prediction, although some variables did significantly improve the model fit. In contrast, clinical variables mapping to cardiometabolic syndrome did independently contribute to the prediction of PACE values beyond the baseline model. The PACE values were poorly correlated with the GrimAge values (r = 0.2), with little overlap in variance explained other than that conveyed by smoking and drinking. The results suggest that EA indices may differ in the clinical information that they provide and may have significant limitations as screening tools to guide patient care.
PON1 rs662, rs854560 and TRIB1 rs17321515, rs2954029 Gene Polymorphisms Are Associated with Lipid Parameters in Patients with Unstable Angina
Authors: Malinowski Damian , Safranow Krzysztof , Pawlik Andrzej
Published: July 2024

Acute coronary heart disease (CHD) is mainly caused by the rupture of an unstable atherosclerotic plaque. Many different factors can cause stenosis or even occlusion of the coronary artery lumen, such as vasculitis and platelet aggregation. Our study was performed to assess the association between PON1 rs662, rs854560 and TRIB1 rs17321515, rs2954029 polymorphisms and the risk of CHD, as well as the association between studied polymorphisms and selected clinical parameters affecting the risk of developing ischemic heart disease. A total of 232 patients with unstable angina were enrolled in this study. There were no statistically significant differences in the PON1 rs662, rs854560 and TRIB1 rs17321515, rs2954029 polymorphism distributions between the total study and control groups. Total cholesterol plasma levels were significantly higher in patients with the PON1 rs662 TT genotype compared to those with the CC+TC genotypes, as well as in patients with the PON1 rs854560 TT genotype compared to those with the AA+AT genotypes. LDL plasma levels were significantly increased in patients with the PON1 rs854560 TT genotype compared to those with the AA+AT genotypes. Plasma levels of HDL were significantly decreased in patients with the TRIB1 rs17321515 AA+AG genotypes compared to those with the GG genotype, as well as in patients with the TRIB1 rs2954029 AA+AT genotypes compared to those with the TT genotype. Our results suggest that the analysed polymorphisms are not risk factors for unstable angina in the Polish population. However, the results of this study indicate an association between the PON1 rs662, rs854560 and TRIB1 rs17321515, rs2954029 polymorphisms with lipid parameters in patients with coronary artery disease.
The Evolution of Genetic Variability at the LRRK2 Locus
Authors: Guenther Dylan T. , Follett Jordan , Amouri Rim , Sassi Samia Ben , Hentati Faycel , Farrer Matthew J.
Published: July 2024

Leucine-rich repeat kinase 2 (LRRK2) c.6055G>A (p.G2019S) is a frequent cause of Parkinson’s disease (PD), accounting for >30% of Tunisian Arab-Berber patients. LRRK2 is widely expressed in the immune system and its kinase activity confers a survival advantage against infection in animal models. Here, we assess haplotype variability in cis and in trans of the LRRK2 c.6055G>A mutation, define the age of the pathogenic allele, explore its relationship to the age of disease onset (AOO), and provide evidence for its positive selection.
Analysis of the Impact Area of the 2022 El Tejado Ravine Mudflow (Quito, Ecuador) from the Sedimentological and the Published Multimedia Documents Approach
Authors: Troncoso Liliana , Torrijo Francisco Javier , Ibadango Elias , Pilatasig Luis , Alonso-Pandavenes Olegario , Mateus Alex , Solano Stalin , Cañar Ruber , Rondal Nicolás , Viteri Francisco
Published: July 2024

Quito (Ecuador) has a history of mudflow events from ravines that pose significant risks to its urban areas. Located close to the Pichincha Volcanic Complex, on 31 January 2022, the northwest and central parts of the city were hit by a mudflow triggered by unusual rainfall in the upper part of the drainage, with 28 fatalities and several properties affected. This research focuses on the affected area from collector overflow to the end, considering sedimentological characteristics and behavior through various urban elements. This study integrates the analysis of videos, images, and sediment deposits to understand the dynamics and impacts of the mudflow using a multidisciplinary approach. The methodology includes verifying multimedia materials using free software alongside the Large-Scale Particle Image Velocimetry (LSPIV) to estimate the kinematic parameters of the mudflow. The affected area, reaching a maximum distance of 3.2 km from the overflow point, was divided into four zones for a detailed analysis, each characterized by its impact level and sediment distribution. Results indicate significant variations in mudflow behavior across different urban areas, influenced by topographical and anthropogenic factors. Multimedia analysis provided insights into the mudflow’s velocity and evolution as it entered urban areas. The study also highlights the role of urban planning and infrastructure in modifying the mudflow’s distribution, particularly in the Northern and Southern Axes of its path, compared with a similar 1975 event, seven times larger than this. It also contributes to understanding urban mudflow events in Quito, offering valuable insights for disaster risk management in similar contexts.
Addressing the Effect of Intra-Seasonal Variations in Developing Rainfall Thresholds for Landslides: An Antecedent Rainfall-Based Approach
Authors: Vishnu Chakrapani Lekha , Oommen Thomas , Chatterjee Snehamoy , Sajinkumar Kochappi Sathyan
Published: July 2024

We developed a rainfall threshold model with the objective of limiting the effects of uncertainties typically associated with them, such as a lack of robust landslide database, the selection of the contributing rain gauge, seasonal variations in rainfall patterns, and the effect of extreme rainfall conditions. With the aid of gauge-corrected satellite precipitation data and a landslide database compiled from various sources, separate rainfall thresholds were developed for two waves of the monsoon season in the Western Ghats, India. The daily vs. antecedent rainfall distributions for different scenarios of antecedent rainfall were analyzed for landslide occurrence. The different scenarios considered included 1, 2, 3, 5, 10-, 20-, 30- and 40-day antecedent rainfalls along with the monsoon antecedent defined as the cumulative rainfall from the start of the monsoon to the day prior to landslide occurrence, and the event antecedent defined as the cumulative rainfall from the start of a rainfall event to the day prior to landslide occurrence. A statistically defined critical value was used to define the thresholds for extreme rainfall conditions, while ordinary least squares and quantile regression models were compared to identify the best-fit model for the non-extreme rainfall threshold. Receiver Operating Characteristic (ROC) analysis was performed on all these models and the best model was chosen based on the efficiency values. The daily vs. monsoon antecedent threshold was the best model for the first monsoon wave, and the daily vs. event antecedent model was the best model for the second monsoon wave. A separate rainfall threshold was defined for the entire monsoon without subdivision into separate waves, and corresponding ROC statistics were compared with the former approach to analyze the efficacy of intra-seasonal variations in rainfall threshold development. The results suggest that cumulative rainfall makes a significant contribution towards landslide initiation and that intra-seasonal variations should be necessarily considered in rainfall threshold modeling.
DIPA: Adversarial Attack on DNNs by Dropping Information and Pixel-Level Attack on Attention
Authors: Liu Jing , Liu Huailin , Wang Pengju , Wu Yang , Li Keqin
Published: July 2024

Deep neural networks (DNNs) have shown remarkable performance across a wide range of fields, including image recognition, natural language processing, and speech processing. However, recent studies indicate that DNNs are highly vulnerable to well-crafted adversarial samples, which can cause incorrect classifications and predictions. These samples are so similar to the original ones that they are nearly undetectable by human vision, posing a significant security risk to DNNs in the real world due to the impact of adversarial attacks. Currently, the most common adversarial attack methods explicitly add adversarial perturbations to image samples, often resulting in adversarial samples that are easier to distinguish by humans. To address this issue, we are motivated to develop more effective methods for generating adversarial samples that remain undetectable to human vision. This paper proposes a pixel-level adversarial attack method based on attention mechanism and high-frequency information separation, named DIPA. Specifically, our approach involves constructing an attention suppression loss function and utilizing gradient information to identify and perturb sensitive pixels. By suppressing the model’s attention to the correct classes, the neural network is misled to focus on irrelevant classes, leading to incorrect judgments. Unlike previous studies, DIPA enhances the attack of adversarial samples by separating the imperceptible details in image samples to more effectively hide the adversarial perturbation while ensuring a higher attack success rate. Our experimental results demonstrate that under the extreme single-pixel attack scenario, DIPA achieves higher attack success rates for neural network models with various architectures. Furthermore, the visualization results and quantitative metrics illustrate that the DIPA can generate more imperceptible adversarial perturbation.
Multiscale Modeling of Elastic Waves in Carbon-Nanotube-Based Composite Membranes
Authors: Mahrous Elaf N. , Hawwa Muhammad A. , Abubakar Abba A. , Al-Qahtani Hussain M.
Published: July 2024

A multiscale model is developed for vertically aligned carbon nanotube (CNT)-based membranes that are made for water purification or gas separation. As a consequence of driving fluids through the membranes, they carry stress waves along the fiber direction. Hence, a continuum mixture theory is established for a representative volume element to characterize guided waves propagating in a periodically CNT-reinforced matrix material. The obtained coupled governing equations for the CNT-based composite are found to retain the integrity of the wave propagation phenomenon in each constituent, while allowing them to coexist under analytically derived multiscale interaction parameters. The influence of the mesoscale characteristics on the continuum behavior of the composite is demonstrated by dispersion curves of harmonic wave propagation. Analytically established continuum mixture theory for the CNT-based composite is strengthened by numerical simulations conducted in COMSOL for visualizing mode shapes and wave propagation patterns.
A Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Didymella segeticola Causing Tea Leaf Spot
Authors: Tu Yiyi , Wang Yuchun , Jiang Hong , Ren Hengze , Wang Xinchao , Lv Wuyun
Published: July 2024

Tea leaf spot caused by Didymella segeticola is an important disease that threatens the healthy growth of tea plants (Camellia sinensis) and results in reductions in the productivity and quality of tea leaves. Early diagnosis of the disease is particularly important for managing the infection. Loop-mediated isothermal amplification (LAMP) assay is an efficient diagnostic technique with the advantages of simplicity, specificity, and sensitivity. In this study, we developed a rapid, visual, and high-sensitivity LAMP assay for D. segeticola detection based on sequence-characterized amplified regions. Two pairs of amplification primers (external primers F3 and B3 and internal primers FIP and BIP) were designed based on a specific sequence in D. segeticola (NCBI accession number: OR987684). Compared to common pathogens of other genera in tea plants and other species in the Didymella genus (Didymella coffeae-arabicae, Didymella pomorum, and Didymella sinensis), the LAMP method is specific for detecting the species D. segeticola. The assay was able to detect D. segeticola at a minimal concentration of 1 fg/μL genomic DNA at an optimal reaction temperature of 65 °C for 60 min. When healthy leaves were inoculated with D. segeticola in the laboratory, the LAMP method successfully detected D. segeticola in diseased tea leaves at 72 h post inoculation. The LAMP assays were negative when the DNA samples were extracted from healthy leaves. Leaf tissues with necrotic lesions from 18 germplasms of tea plants tested positive for the pathogen by the LAMP assay. In summary, this study established a specific, sensitive, and simple LAMP method to detect D. segeticola, which provides reliable technical support for estimating disease prevalence and facilitates sustainable management of tea leaf spot.