bluuebunny/crossref_metadata_2025 huggingface dataset:
Age and Origin of the Massangana Intrusive Suite and Associated Mineralizations, in the Rondônia Tin Province: Petrography, U-Pb, and Lu-Hf Isotopes Zircons
Authors: Debowski Beatriz , Potratz Guilherme , Tavares Júnior Armando , Alves Martins Maria , Geraldes Mauro
Published: October 2022

Rondônia intrusive suites represent the youngest A-type magmatism that occurred in the SW of the Amazon craton, with mineralizations in Sn, Nb, Ta, W, and topaz. Petrological and isotopic studies (U-Pb and Lu-Hf by LA-ICP-MS) allowed the Massangana granite to be subdivided into São Domingos facies (medium to fine biotite-granite), Bom Jardim facies (fine granite), Massangana facies (pyterlites and coarse granites) and Taboca facies (fine granites). The crystallization ages obtained were between 995.7 ± 9.5 Ma to 1026 ± 16 Ma, and the εHf values vary significantly between positive and negative, showing predominantly crustal sources for forming these rocks. Petrographic studies on ore samples indicate the action of co-magmatic hydrothermal fluids enriched in CO2, H2O, and F. These ores are characterized by endogreisens, exogreisens, pegmatites, and quartz veins that are explored in the São Domingos facies area. The endogreisens and exogreisens are formed by topaz-granites and zinnwaldite-granites; the pegmatites are formed by topaz-zinnwaldite-cassiterite-granites; and the veins by cassiterite-sulfides and quartz. The geometries of the mineralized bodies indicate a dome-shaped contact with the host rocks in the magma chamber and can be attributed to residual accumulation. In this sense, the origin of these ores is related to the evolution of intrusive granitic bodies where the terminal phases of the fluid-enriched magma are lodged in the apical portions, and the origin of the mineralized bodies present a biotite-granite, albite-granite, and endogreisens evolution (potassium series), or biotite-granite, alkali-granite and endogreisens (sodic series) and these rocks present TDM ages that indicate a concerning relation to the non-mineralized rocks of Massangana granite.
Application Study of Empirical Wavelet Transform in Time–Frequency Analysis of Electromagnetic Radiation Induced by Rock Fracture
Authors: Lou Quan , Wan Xiangyun , Jia Bing , Song Dazhao , Qiu Liming , Yin Shan
Published: October 2022

The time–frequency characteristics of electromagnetic radiation (EMR) waveform induced by rock fracture are very important to the monitoring and early–warning using the EMR method for the mine rockburst. The empirical wavelet transform (EWT), as a waveform time–frequency analysis method, has the advantages of a clear theoretical basis, convenient calculation, and no modal aliasing. To apply EWT to the field of EMR time–frequency analysis, the operation of Fourier axis segmentation of EWT is improved. In detail, the adaptive selection method for a window width of closing operation and the adaptive determination method of segment number of Fourier axis are proposed for EWT. The Fourier axis obtained by short–time Fourier transform (STFT) is used in the EWT process, rather than that obtained by discrete Fourier transform (DFT), taking a better Fourier axis segmentation effect. The improved EWT together with Hilbert transform (HT) applied to the time–frequency analysis for the EMR waveform of rock fracture, and the time–frequency spectrum obtained by EWT–HT can well describe the time–frequency evolution characteristics. Compared with STFT and Hilbert–Huang transform (HHT), EWT–HT has significant advantages in time–frequency resolution and overcoming modal aliasing, providing a powerful tool for time–frequency analysis for the EMR waveform induced by rock fracture.
A Review on Gallium Oxide Materials from Solution Processes
Authors: Chiang Jung-Lung , Yadlapalli Bharath Kumar , Chen Mu-I , Wuu Dong-Sing
Published: October 2022

Gallium oxide (Ga2O3) materials can be fabricated via various methods or processes. It is often mentioned that it possesses different polymorphs (α-, β-, γ-, δ- and ε-Ga2O3) and excellent physical and chemical properties. The basic properties, crystalline structure, band gap, density of states, and other properties of Ga2O3 will be discussed in this article. This article extensively discusses synthesis of pure Ga2O3, co-doped Ga2O3 and Ga2O3-metal oxide composite and Ga2O3/metal oxide heterostructure nanomaterials via solution-based methods mainly sol-gel, hydrothermal, chemical bath methods, solvothermal, forced hydrolysis, reflux condensation, and electrochemical deposition methods. The influence of the type of precursor solution and the synthesis conditions on the morphology, size, and properties of final products is thoroughly described. Furthermore, the applications of Ga2O3 will be introduced and discussed from these solution processes, such as deep ultraviolet photodetector, gas sensors, pH sensors, photocatalytic and photodegradation, and other applications. In addition, research progress and future outlook are identified.
Crystallisation Kinetics and Associated Electrical Conductivity Dynamics of Poly(Ethylene Vinyl Acetate) Nanocomposites in the Melt State
Authors: Stalmann Gertrud , Matic Aleksandar , Jacobsson Per , Tranchida Davide , Gitsas Antonis , Gkourmpis Thomas
Published: October 2022

Nanocomposite systems comprised of a poly(ethylene vinyl acetate) (EVA) matrix and carbon black (CB) or graphene nanoplatelets (GNPs) were used to investigate conductivity and crystallisation dynamics using a commercially relevant melt-state mixing process. Crystallisation kinetics and morphology, as investigated by DSC and SEM, turn out to depend on the interplay of (i) the interphase interactions between matrix and filler, and (ii) the degree of filler agglomeration. For the GNP-based systems, an almost constant conductivity value was observed for all compositions upon cooling, something not observed for the CB-based compositions. These conductivity changes reflect structural and morphological changes that can be associated with positive and negative thermal expansion coefficients. GNP-based systems were observed to exhibit a percolation threshold of approximately 2.2 vol%, lower than the 4.4 vol% observed for the CB-based systems.
A Versatile Route for Synthesis of Metal Nanoalloys by Discharges at the Interface of Two Immiscible Liquids
Authors: Hamdan Ahmad , Stafford Luc
Published: October 2022

Discharge in liquid is a promising technique to produce nanomaterials by electrode erosion. Although its feasibility was demonstrated in many conditions, the production of nanoalloys by in-liquid discharges remains a challenge. Here, we show that spark discharge in liquid cyclohexane that is in contact with conductive solution, made of a combination of Ni-nitrate and/or Fe-nitrate and/or Co-nitrate, is suitable to produce nanoalloys (<10 nm) of Ni-Fe, Ni-Co, Co-Fe, and Ni-Co-Fe. The nanoparticles are synthesized by the reduction of metal ions during discharge, and they are individually embedded in C-matrix; this latter originates from the decomposition of cyclohexane. The results open novel ways to produce a wide spectrum of nanoalloys; they are needed for many applications, such as in catalysis, plasmonic, and energy conversion.
Synthesis and Electrochemical Performance of Microporous Hollow Carbon from Milkweed Pappus as Cathode Material of Lithium–Sulfur Batteries
Authors: Kim Jun-Ki , Choi Yunju , Jeong Euh Duck , Lee Sei-Jin , Kim Hyun Gyu , Chung Jae Min , Kim Jeom-Soo , Lee Sun-Young , Bae Jong-Seong
Published: October 2022

Microtube-like porous carbon (MPC) and tube-like porous carbon–sulfur (MPC-S) composites were synthesized by carbonizing milkweed pappus with sulfur, and they were used as cathodes for lithium–sulfur batteries. The morphology and uniformity of these materials were characterized using X-ray powder diffraction, Raman spectroscopy, scanning electron microscopy, transmission electron microscopy with an energy-dispersive X-ray analyzer, thermogravimetric analysis, and X-ray photoelectron spectrometry. The electrochemical performance of the MPC-S cathodes was measured using the charge/discharge cycling performance, C rate, and AC impedance. The composite cathodes with 93.8 wt.% sulfur exhibited a stable specific capacity of 743 mAh g−1 after 200 cycles at a 0.5 C.
Editorial for Special Issue: Advanced Materials and Technologies in Nanogenerators
Authors: Wen Zhen , Guo Hengyu , Wang Longfei
Published: October 2022

Nanogenerators, based on Maxwell’s displacement current as the driving force, have inspired a new and developing field since their invention in 2006 [...]
Manufacturing Bulk Nanocrystalline Al-3Mg Components Using Cryomilling and Spark Plasma Sintering
Authors: Kushwaha Amanendra K. , Misra Manoranjan , Menezes Pradeep L.
Published: October 2022

In the current study, pure aluminum (Al) powders were cryomilled with and without 3 wt.% pure magnesium (Mg) dopant for varying durations followed by spark plasma sintering (SPS) of powders to prepare bulk components with superior mechanical properties. The crystallite sizes were determined for powders and the bulk components by analyzing the X-ray diffraction (XRD) spectrum. The calculations indicated a reduction in crystallite size with the increase in the cryomilling duration. The results also showed a more significant decrease in the crystallite sizes for Al-3Mg samples than that of pure Al. The changes in the surface morphology of powders were characterized using scanning electron microscopy (SEM). The elemental mapping analysis at nanoscale was carried out using Energy-dispersive X-ray spectroscopy (EDX) in Scanning transmission electron microscopy (STEM). The mechanical properties of the bulk components were assessed using a Vickers Microhardness tester. The test results demonstrated an improvement in the hardness of Mg-doped components. Higher hardness values were also reported with an increase in the cryomilling duration. This article discusses the mechanisms for the reduction in crystallite size for pure Al and Al-3Mg and its subsequent impact on improving mechanical properties.
Transfer Learning for Modeling Plasmonic Nanowire Waveguides
Authors: Luo Aoning , Feng Yuanjia , Zhu Chunyan , Wang Yipei , Wu Xiaoqin
Published: October 2022

Retrieving waveguiding properties of plasmonic metal nanowires (MNWs) through numerical simulations is time- and computational-resource-consuming, especially for those with abrupt geometric features and broken symmetries. Deep learning provides an alternative approach but is challenging to use due to inadequate generalization performance and the requirement of large sets of training data. Here, we overcome these constraints by proposing a transfer learning approach for modeling MNWs under the guidance of physics. We show that the basic knowledge of plasmon modes can first be learned from free-standing circular MNWs with computationally inexpensive data, and then reused to significantly improve performance in predicting waveguiding properties of MNWs with various complex configurations, enabling much smaller errors (~23–61% reduction), less trainable parameters (~42% reduction), and smaller sets of training data (~50–80% reduction) than direct learning. Compared to numerical simulations, our model reduces the computational time by five orders of magnitude. Compared to other non-deep learning methods, such as the circular-area-equivalence approach and the diagonal-circle approximation, our approach enables not only much higher accuracies, but also more comprehensive characterizations, offering an effective and efficient framework to investigate MNWs that may greatly facilitate the design of polaritonic components and devices.
Multiple Resistive Switching Mechanisms in Graphene Oxide-Based Resistive Memory Devices
Authors: Koveshnikov Sergei , Kononenko Oleg , Soltanovich Oleg , Kapitanova Olesya , Knyazev Maxim , Volkov Vladimir , Yakimov Eugene
Published: October 2022

Among the different graphene derivatives, graphene oxide is the most intensively studied material as it exhibits reliable and repeatable resistive switching. The operative mechanisms that are responsible for resistive switching are being intensively investigated, and three models explaining the change in the resistive states have been developed. These models are grounded in the metallic-like filamentary conduction, contact resistance modification and the oxidation of/reduction in the graphene oxide bulk. In this work, using Al/GO/n-Si structures, we demonstrate that all three of these operative mechanisms can simultaneously participate in the resistive switching of graphene oxide. Multiple point-like conduction channels in the graphene oxide films were detected by the electron beam-induced current (EBIC) technique. At the same time, large areas with increased conductivity were also revealed by EBIC. An analysis of these areas by Raman spectroscopy indicates the change in the graphene oxide bulk’s resistive properties. The EBIC data along with the measurements of the capacitance–voltage characteristics provided strong evidence of the involvement of an aluminum/graphene oxide interface in the switching processes. In addition, by using Al/GO/n-Si structures, we were able to identify unique local properties of the formed conductive channels, namely the change of the charge state of a conductive channel due to the creation of negatively charged traps and/or an increase in the GO work function.
Heat Transfer Enhancement in the Microscale: Optimization of Fluid Flow
Authors: Beck Joshua , Palmer Michael , Inman Kallie , Wohld Jake , Cummings Marcus , Fulmer Ryan , Scherer Branden , Vafaei Saeid
Published: October 2022

The focus of this paper is to investigate the effects of the addition of a connector between two serial microchannels. The idea of adding connector at the inlet of microchannels to enhance the random motion of molecules or nanoparticles in low Reynolds numbers was developed in our research group for the first time. It was experimentally determined that the shape of a connector between two microchannels has a significant impact on the enhancement of the random motion of molecules or nanoparticles. Consequently, the heat transfer coefficient is improved inside the second microchannel. The connector is large enough to refresh the memory of the fluid before entering the second channel, causing a higher maximum heat transfer coefficient in the second channel. It was also observed that the heat transfer coefficient can be increased at the end of the channel when the outlet temperature is relatively high. This may be explained by the fact that as temperature increases, the fluid viscosity tends to decrease, which generally drives an increase in the local random motion of base fluid molecules and nanoparticles. This causes an increase in the microchannel heat transfer coefficient. It was found that the addition of nanoparticles significantly modified the impact of the connector on the microchannel heat transfer coefficient. In addition, the effects of changing the Reynolds number and the shape of the connector were investigated through use of computational fluid dynamics (CFD) calculations. It was found that both factors have an important impact on the variation of velocity and enhancement of random motion of molecules and consequently significantly affect the heat transfer coefficient.
Sustainable Carbon Derived from Sulfur-Free Lignins for Functional Electrical and Electrochemical Devices
Authors: Thomas Bony , Sain Mohini , Oksman Kristiina
Published: October 2022

Technical lignins, kraft, soda, lignoboost, and hydrolysis lignins were used for the production of carbon particles at different carbonization temperatures, 1000 °C and 1400 °C. The results showed that the lignin source and carbonization temperature significantly influenced the carbon quality and microstructure of the carbon particles. Soda lignin carbonized up to 1400 °C showed higher degree of graphitization and exhibited the highest electrical conductivity of 335 S·m−1, which makes it suitable for applications, such as electromagnetic interference shielding and conductive composite based structural energy storage devices. The obtained carbon particles also showed high surface area and hierarchical pore structure. Kraft lignin carbonized up to 1400 °C gives the highest BET surface area of 646 m2 g−1, which makes it a good candidate for electrode materials in energy storage applications. The energy storage application has been validated in a three-electrode set up device, and a specific capacitance of 97.2 F g−1 was obtained at a current density of 0.1 A g−1 while an energy density of 1.1 Wh kg−1 was observed at a power density of 50 W kg−1. These unique characteristics demonstrated the potential of kraft lignin-based carbon particles for electrochemical energy storage applications.
Organo-Vermiculites Modified by Aza-Containing Gemini Surfactants: Efficient Uptake of 2-Naphthol and Bromophenol Blue
Authors: Gong Jianchao , Wang Tingting , Zhang Wei , Han Lin , Gao Mingxiao , Chen Tianen , Shen Tao , Ji Yaxiong
Published: October 2022

To explore the effect of spacer structure on the adsorption capability of organo-vermiculites (organo-Vts), a series of aza-containing gemini surfactants (5N, 7N and 8N) are applied to modify Na-vermiculite (Na-Vt). Large interlayer spacing, strong binding strength and high modifier availability are observed in organo-Vts, which endow them with superiority for the adsorption of 2-naphthol (2-NP) and bromophenol blue (BPB). The maximum adsorption capacities of 5N-Vt, 7N-Vt and 8N-Vt toward 2-NP/BPB are 142.08/364.49, 156.61/372.65 and 146.50/287.90 mg/g, respectively, with the adsorption processes well fit by the PSO model and Freundlich isotherm. The quicker adsorption equilibrium of 2-NP than BPB is due to the easier diffusion of smaller 2-NP molecules into the interlayer space of organo-Vts. Moreover, stable regeneration of 7N-Vt is verified, with feasibility in the binary-component system that is demonstrated. A combination of theoretical simulation and characterization is conducted to reveal the adsorption mechanism; the adsorption processes are mainly through partition processes, electrostatic interaction and functional interactions, in which the spacer structure affects the interlayer environment and adsorptive site distribution, whereas the adsorbate structure plays a role in the diffusion process and secondary intermolecular interactions. The results of this study demonstrate the versatile applicability of aza-based organo-Vts targeted at the removal of phenols and dyes as well as provide theoretical guidance for the structural optimization and mechanistic exploration of organo-Vt adsorbents.
Molecular Effects of Iodine-Biofortified Lettuce in Human Gastrointestinal Cancer Cells
Authors: Sularz Olga , Koronowicz Aneta , Boycott Cayla , Smoleń Sylwester , Stefanska Barbara
Published: October 2022

Considering the growing number of cancer cases around the world, natural products from the diet that exhibit potential antitumor properties are of interest. Our previous research demonstrated that fortification with iodine compounds is an effective way to improve the antioxidant potential of lettuce. The purpose of the present study was to evaluate the effect of iodine-biofortified lettuce on antitumor properties in human gastrointestinal cancer cell lines, gastric AGS and colon HT-29. Our results showed that extracts from iodine-biofortified lettuce reduce the viability and proliferation of gastric and colon cancer cells. The extracts mediated cell cycle arrest which was accompanied by inactivation of anti-apoptotic Bcl-2 and activation of caspases, as assessed by flow cytometry. However, extracts from lettuce fortified with organic forms of iodine acted more effectively than extracts from control and KIO3-enriched plants. Using quantitative PCR, we detected the increase in pro-apoptotic genes BAD, BAX and BID in AGS cells whereas up-regulation of cell cycle progression inhibitor CDKN2A and downregulation of pro-proliferative MDM2 in HT-29 cells. Interestingly, lettuce extracts led to down-regulation of pro-survival AKT1 and protooncogenic MDM2, which was consistent for extracts of lettuce fortified with organic form of iodine, 5-ISA, in both cell lines. MDM2 downregulation in HT-29 colon cancer cells was associated with RB1 upregulation upon 5-ISA-fortified lettuce extracts, which provides a link to the epigenetic regulation of tumor suppressor genes by RB/MDM2 pathway. Indeed, SEMA3A tumor suppressor gene was hypomethylated and upregulated in HT-29 cells treated with 5-ISA-fortified lettuce. Control lettuce exerted similar effects on RB/MDM2 pathway and SEMA3A epigenetic activation in HT-29 cells. Our findings suggest that lettuce as well as lettuce fortified with organic form of iodine, 5-ISA, may exert epigenetic anti-cancer effects that can be cancer type-specific.
Importance of Intermittent Fasting Regimens and Selection of Adequate Therapy on Inflammation and Oxidative Stress in SARS-CoV-2 Infection
Authors: Ezzati Armin , Rosenkranz Sara K. , Horne Benjamin D.
Published: October 2022

The unpredictable nature of new variants of coronavirus 2 (SARS-CoV-2)—highly transmissible and some with vaccine-resistance, have led to an increased need for feasible lifestyle modifications as complementary therapies. Systemic inflammation is the common hallmark of communicable diseases like severe coronavirus disease 2019 (COVID-19) and non-communicable chronic diseases (NCDs) such as obesity, cardiovascular diseases (CVD), diabetes mellitus, and cancers, all for which mitigation of severe outcomes is of paramount importance. Dietary quality is associated with NCDs, and intermittent fasting (IF) has been suggested as an effective approach for treatment and prevention of some NCDs, similar to that of caloric restriction. There is a paucity of high-quality data from randomized controlled trials regarding the impact of IF and the intake of specific nutrients on inflammation and post-infection outcomes in patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The current review of recent literature was performed to explore the immunomodulatory roles of IF regimens and supplements involving the intake of specific nutrients including vitamins (A, B, C, D, and E), zinc, and nutraceuticals (n-3 polyunsaturated fatty acids, quercetin, and probiotics) on inflammatory and oxidative stress markers, with consideration of how they may be related to SARS-CoV-2.
Probiotics in the Prevention and Treatment of Gestational Diabetes Mellitus (GDM): A Review
Authors: Kamińska Klaudia , Stenclik Dominika , Błażejewska Wiktoria , Bogdański Paweł , Moszak Małgorzata
Published: October 2022

Gestational diabetes mellitus (GDM)is one of the most common perinatal pathologies, with a prevalence of 5–20% depending on the population or diagnostic standards. It is diagnosed when glucose intolerance is first detected during pregnancy. In the pathogenesis of GDM, genetic, environmental, and pregnancy-related factors (excessive fat storage and increased adipokine and cytokine secretion) play important roles. A growing amount of scientific data has indicated the role of gut microbiota (GM) dysbiosis in the development of glucose intolerance during pregnancy. Previous studies have indicated that, in comparison to healthy pregnant women, GDM individuals have a greater abundance of bacteria belonging to the genera Ruminococcus, Eubacterium, and Prevotella and a lower number of bacteria belonging to the genera Bacteroides, Parabacteroides, Roseburia, Dialister, and Akkermansia. Recently, many studies have focused on treating GDM with methods targeting GM. Several previous studies have analyzed the effect of probiotics on the course of GDM, but their data are inconclusive. In view of this state, the aim of the study was to collect and comprehensively discuss current knowledge regarding the role of probiotic supplementation in preventing and treating GDM. According to the analyzed data, probiotics have a positive influence on glycemic control and are a promising tool for lowering the frequency of GDM. However, further studies must be conducted to determine the optimal model of probiotic therapy (strain, dose, time of intervention, etc.) in pregnant women with GDM.
Diet Quality and Level of Nutrition Knowledge among Young People with Orthorexic Tendencies
Authors: Kaźmierczak-Wojtaś Natalia , Drozd Mariola
Published: October 2022

The aim of the study was to determine the level of nutrition knowledge and diet quality, understood in terms of healthy and unhealthy eating habits, among young people with orthorexic tendencies. The participants were school students, university students, and those employed in the Lublin region (N = 473). The data were collected by means of a questionnaire. The participants were asked to provide socio-demographic data through filling in the ORTO-15 questionnaire and the Dietary Habits and Nutrition Beliefs Questionnaire (KomPAN). The participants obtained results ranging from 9.3 to 100 (M = 31.15; SD = 11.81) in the non-healthy diet index, from 0.4 to 78.6 in the pro-healthy diet index (M = 21.79; SD = 11.08), and from 0 to 23 in the domain of nutrition knowledge (M = 13; SD = 4.23). A variance analysis showed no significant differences between the pro-healthy diet index and the level of nutrition knowledge. The group with orthorexia obtained statistically higher results in the pro-healthy diet index. Those with a tendency toward orthorexia obtained statistically higher results in the non-healthy diet index. The variance analysis showed that the level of nutrition knowledge of those not focused on healthy foods was significantly lower than in the other groups. The results of the ORTO-15 questionnaire correlated negatively with the pro-healthy diet index and the level of nutrition knowledge, and positively with the non-healthy diet index. We concluded that: 1. the orthorexic group and the group with a tendency toward orthorexia could be characterized with a moderate intensity of a healthy diet and a low intensity of a non-healthy diet; and 2. the level of nutrition knowledge in the orthorexic group did not significantly differ from that of the other groups.
Malaria Detection Accelerated: Combing a High-Throughput NanoZoomer Platform with a ParasiteMacro Algorithm
Authors: Ashraf Shoaib , Khalid Areeba , de Vos Arend L. , Feng Yanfang , Rohrbach Petra , Hasan Tayyaba
Published: October 2022

Eradication of malaria, a mosquito-borne parasitic disease that hijacks human red blood cells, is a global priority. Microscopy remains the gold standard hallmark for diagnosis and estimation of parasitemia for malaria, to date. However, this approach is time-consuming and requires much expertise especially in malaria-endemic countries or in areas with low-density malaria infection. Thus, there is a need for accurate malaria diagnosis/parasitemia estimation with standardized, fast, and more reliable methods. To this end, we performed a proof-of-concept study using the automated imaging (NanoZoomer) platform to detect the malarial parasite in infected blood. The approach can be used as a steppingstone for malaria diagnosis and parasitemia estimation. Additionally, we created an algorithm (ParasiteMacro) compatible with free online imaging software (ImageJ) that can be used with low magnification objectives (e.g., 5×, 10×, and 20×) both in the NanoZoomer and routine microscope. The novel approach to estimate malarial parasitemia based on modern technologies compared to manual light microscopy demonstrated 100% sensitivity, 87% specificity, a 100% negative predictive value (NPV) and a 93% positive predictive value (PPV). The manual and automated malaria counts showed a good Pearson correlation for low- (R2 = 0.9377, r = 0.9683 and p < 0.0001) as well as high- parasitemia (R2 = 0.8170, r = 0.9044 and p < 0.0001) with low estimation errors. Our robust strategy that identifies and quantifies malaria can play a pivotal role in disease control strategies.
Matrix Metalloproteinase 9 (MMP-9) and Interleukin-8 (IL-8) in Gingival Crevicular Fluid after Regenerative Therapy in Periodontal Intrabony Defects with and without Systemic Antibiotics—Randomized Clinical Trial
Authors: Dolińska Ewa , Pietruska Małgorzata , Dymicka-Piekarska Violetta , Milewski Robert , Sculean Anton
Published: October 2022

The aim of our study was to assess changes in the levels of IL-8 and MMP-9 in gingival crevicular fluid (GCF) collected from the periodontal pocket before and after regenerative surgery with deproteinized bovine bone mineral (DBBM) and collagen membrane (GTR) either independently (DBBM/GTR) or with the postoperative administration of antibiotic (DBBM/GTR+AB). The study involved 41 patients, each with one intrabony defect. IL-8 and MMP-9 were determined before therapy and after 2 weeks, 4 weeks and 6 months following the surgical procedure by means of dedicated ELISA kits. No statistical differences were observed in the levels of IL-8 and MMP-9 after 2 weeks, 4 weeks and 6 months between the groups. The changes in the level of MMP-9 over time were not statistically significant in any group. The changes in the level of IL-8 were significant for the group given antibiotic but not in the nonantibiotic group in the follow-up period. IL-8 and MMP-9 were found to correlate positively but not after 4 weeks in the test group. Current assessment of IL-8 and MMP-9 obtained from GCF samples provides evidence that collagen matrix turnover occurs actively during the early healing phase in the periodontium after regenerative procedures. We observed positive correlations of MMP-9 and IL-8 throughout the study. However, we failed to reveal any differences regard parameters studied between the two groups.
A Comparison of Real and Virtual Laboratories for Pharmacy Teaching
Authors: Schneider Jennifer , Felkai Chelsea , Munro Irene
Published: October 2022

New approaches to teaching and learning in the tertiary setting offer students flexibility for learning and, in a pandemic, suggests ways to provide learning when face-to-face delivery cannot be conducted. Courses that contain a hands-on laboratory component can be resource intensive in terms of equipment, staff, and facilities, thus more difficult to deliver when hands-on laboratory work is precluded. This study developed two virtual laboratories that could be completed online and, using a crossover design, evaluated student learning outcomes from virtual and real laboratory activities for 57 students. It also gained student feedback on their learning experiences. Overall, student knowledge increased significantly for each topic after completing either the virtual or real laboratory activities. However, no significant difference in learning was observed when outcomes from virtual or real laboratories were compared. Feedback from students indicated that most students found online modules easier to follow, they provided better background information, and would be revisited, but real laboratories were more interesting. Reinforcing learning, understanding, and remembering processes were reportedly similar for both, indicating no negative impact when a virtual laboratory was used. This study provides supporting evidence for the use of virtual laboratories where the focus is on learning concepts and not on student proficiency at operating laboratory equipment.
Dual-Wavelength Continuous-Wave and Passively Q-Switched Alexandrite Laser at 736.7 nm and 752.8 nm
Authors: Lin Hongyi , Bao Shangfeng , Liu Xiao , Song Shuo , Wen Zhiwei , Sun Dong
Published: October 2022

A dual-wavelength continuous-wave (CW) and passively Q-switched alexandrite laser based on a MoS2 saturable absorber (SA) operating at 736.7 nm and 752.8 nm with a simple and compact 18 mm plano–plano resonator is reported. In the CW mode, the output power is 1014 mW at the linear-polarized pump power of 5.44 W, with a slope efficiency of 28.7%. In the pulsed operation, the narrowest pulse width and the maximal peak power are 154 ns and 10.6 W, respectively. This laser can be used to generate 8.71 THz-wave light based on a suitable nonlinear optical crystal.
In Vitro Sprouted Plantlets of Citrullus colocynthis (L.) Schrad Shown to Possess Interesting Levels of Cucurbitacins and Other Bioactives against Pathogenic Fungi
Authors: Marzouk Belsem , Refifà Meher , Montalbano Serena , Buschini Annamaria , Negri Stefano , Commisso Mauro , Degola Francesca
Published: October 2022

Cucurbitacins, structurally different triterpenes mainly found in the members of Cucurbitaceae, possess a vast pharmacological potential. Genus Cucurbita, Cucumis, and Citrullus are affluent in these bioactive compounds, and, amongst them, Citrullus colocynthis (L.) Schrad. is widely exploited in folk medicine, since a huge number of diseases are successfully treated with organic and aqueous extracts obtained from different organs and tissues of the plant. The well-known pharmacological activities of such species have been attributed to its peculiar composition, which includes cucurbitacins and other bioactive molecules; thus, owing to its high importance as a valuable natural resource for pharmaceuticals and nutraceuticals, C. colocynthis propagation and multiplication protocols are considered significant, but the exploitation of its phytochemical potential is limited by the restricted cultivation conditions and the low rate of seed germination in the natural environment; in fact, the assessment of accumulation rate of specific phytochemicals under controlled conditions is still missing. Axenically sprouted plantlets obtained without the use of culture media or the addition of hormones have been evaluated here for the production of bioactive compounds and relevant bioactive features. Our results proved that derived organic extracts contain cucurbitacins and other bioactives, show antioxidant potential, and exert activity against some pathogenic fungi (Candida krusei, C. albicans, C. parapsilosis, C. glabrata, and Aspergillus flavus), supporting the feasibility of a methodology intended to scale-up cultivation of this species as a source of pharmaceutically interesting compounds, achievable from plantlets cultivated under laboratory conditions.
Melatonin Preserves the Postharvest Quality of Cut Roses through Enhancing the Antioxidant System
Authors: Mazrou Ragia M. , Hassan Sabry , Yang Mei , Hassan Fahmy A.S.
Published: October 2022

The vase life of cut rose is relatively short, therefore; preserving its postharvest quality via eco-friendly approaches is of particular economic importance. From the previous literature, despite melatonin (MT) plays diverse important roles in the postharvest quality maintenance, its impact on preserving the postharvest quality of cut flowers is really scarce. This research therefore was undertaken to find out the possibility of exogenous MT as an eco-friendly preservative to extend the vase life of cut roses. The flowering stems of Rosa hybrida cv. ‘First Red’ were pulsed in MT solutions at 0, 0.1, 0.2 and 0.3 mM for 30 min and then transferred to distilled water for evaluation. The vase life was significantly prolonged and relative water content was considerably maintained due to MT application compared to the control, more so with 0.2 mM concentration which nearly doubled the vase life (1.9-fold) higher than the control. SEM investigation showed that MT treatment reduced the stomatal aperture in lower epidermis which was widely opened in control flowers. MT treatment significantly increased the phenol content, glutathione (GSH) content and CAT, APX and GR enzyme activities compared to untreated flowers. Additionally, the radical scavenging capacity in MT-treated flowers was considerably higher than that of control and therefore MT treatment reduced H2O2 production and lipid peroxidation, which altogether reflected in membrane stability maintenance.
Germination Behavior and Early Seedling Growth in Abies pinsapo Boiss. Seeds
Authors: Bravo-Navas María Victoria , Sánchez-Romero Carolina
Published: October 2022

Abies pinsapo Boiss. is a conifer endemic to southern Spain. It is categorized as an endangered species in the IUCN list and the plant communities it forms are considered unique ecosystems, being the remains of fir forests occupying the Mediterranean basin during the last glaciations. Understanding seed germination and plant production is essential for the management and conservation of A. pinsapo stands. The objective of this work was to investigate the effect of temperature and light on germination behavior and early seedling growth of A. pinsapo seeds from different populations. The results obtained reveal a significant influence of seed origin on germination percentage. Temperature played a critical role on germination rate, with optimal results at 15 °C. Light only significantly affected germination in seeds from Grazalema, although significant population × light and temperature x light interactions could be inferred. In relation to germination kinetics, different responses to the environmental factors tested were observed among seeds from different provenances. Globally, the temperature influenced all the germination parameters, except germination onset. However, light affected germination initiation and speed. Early seedling growth depended on seed origin and temperature. The temperature played a determinant role as temperatures above 15 °C strongly limited plantlets development. Light only significantly influenced root length in plantlets obtained from Grazalema seeds.
Extracts from Frangula alnus Mill. and Their Effects on Environmental and Probiotic Bacteria
Authors: Kledecka Agata , Siejak Przemysław , Pratap-Singh Anubhav , Kowalczewski Przemysław Łukasz , Fathordoobady Farahnaz , Jarzębski Maciej , Smułek Wojciech
Published: October 2022

The bark of Frangula alnus Mill (FAM), the so-called alder buckthorn, has been widely investigated for its medicinal properties, especially its laxative effects and the bioactive properties of the plant material extract. Still, there is no wider study devoted to its antibacterial properties. This is important in the context of its impact on probiotic gut bacteria. The aim of the research was to recognize the effect of FAM extract on bacterial cells, and to determine how the bioactive properties and composition of the extract are influenced by the type of solvent used for the extraction. To find the most suitable conditions for the FAM extraction, we used four solvent solutions with different polarities, including water, methanol, ethanol, and isopropanol. We assessed the quality and composition of the extracts with spectral analysis, using spectrophotometric (FTIR, UV-Vis) and chromatographic methods (GC-MS). Finally, we analyzed the extractant impact of the extracts on the selected bacterial cells. The results showed that the chemical diversity of the extracts increased with the increase in solvent polarity, in which the abundance of frangulin, the main bioactive compound in buckthorn bark, was confirmed. Pseudomonas fluorescens ATCC 17400 was particularly sensitive to the action of extracts, whereas other strains of the Pseudomonas genus showed practically no adverse effects. Ethanolic extracts had the strongest effect on most of the selected bacteria strains. We found that the probiotic Lactobacillus strain, which represents intestinal microflora, has no direct effect on probiotic microorganisms. The research shown FAM extracts can be safe for probiotic bacteria present in human gut microflora. Moreover, the study indicated that contact with the extracts may reduce the total permeability of the bacterial membranes. This opens up the possibility of using FAM extracts as a factor regulating transport into cells, which may be used to support the action of other bioactive substances.
Maize Lodging Resistance with Plastic Film Removal, Increased Planting Density, and Cultivars with Different Maturity Periods
Authors: Zhang Xiyun , Xue Jun , Tian Ming , Zhang Guoqiang , Ming Bo , Wang Keru , Hou Peng , Xie Ruizhi , Tang Qiuxiang , Li Shaokun
Published: October 2022

While plastic film mulching and proper high-density planting are important methods that can improve maize yield, years of accumulated residual film have created soil pollution and degraded soil, and thus has impeded sustainable agriculture development. Here, we compared the stalk and root lodging resistances of three maize cultivars grown at two planting densities both with (FM) and without (NM) plastic film mulch. Our aim was to provide a theoretical basis that may help assure a future of successful no-film planting with increased planting density. The results showed that, compared with FM, the average dry weight per unit length and bending strength of basal internode decreased for all cultivars at both planting densities in the NM treatment. At 9.0 × 104 plants ha−1, the stalk breaking force (SFC) of Xinyu77, KWS9384, and KWS2030 in the NM treatment decreased by 4%, 21%, and 22%, respectively. At 12.0 × 104 plants ha−1, SFC of Xinyu77 and KWS2030 increased by 14% and 1%, respectively, while KWS9384 decreased by 10%. Additionally, the root diameter, length, volume, width, depth, and the vertical root-pulling force of maize decreased. Although the lodging resistance of maize grown without film mulch was lower than that of maize grown with it, those adverse effects can be mitigated by selecting suitable cultivars and by using proper high-density planting and appropriate cultivation measures.
Melanisation in Boreal Lichens Is Accompanied by Variable Changes in Non-Photochemical Quenching
Authors: Ndhlovu Nqobile Truelove , Solhaug Knut Asbjørn , Minibayeva Farida , Beckett Richard Peter
Published: October 2022

Lichens often grow in microhabitats where they absorb more light than they can use for fixing carbon, and this excess energy can cause the formation of harmful reactive oxygen species (ROS). Lichen mycobionts can reduce ROS formation by synthesizing light-screening pigments such as melanins in the upper cortex, while the photobionts can dissipate excess energy radiationlessly using non-photochemical quenching (NPQ). An inherent problem with using fluorimetry techniques to compare NPQ in pale and melanised thalli is that NPQ is normally measured through a variously pigmented upper cortex. Here we used a dissection technique to remove the lower cortices and medullas of Lobaria pulmonaria and Crocodia aurata and then measure NPQ from the underside of the thallus. Results confirmed that NPQ can be satisfactorily assessed with a standard fluorimeter by taking measurement from above using intact thalli. However, photobionts from the bottom of the photobiont layer tend to have slightly lower rates of PSII activity and lower NPQ than those at the top, i.e., display mild “shade” characteristics. Analysis of pale and melanised thalli of other species indicates that NPQ in melanised thalli can be higher, similar or lower than pale thalli, probably depending on the light history of the microhabitat and presence of other tolerance mechanisms.
Phosphoprotein Profile of Rice (Oryza sativa L.) Seedlings under Osmotic Stress after Pretreatment with Chitosan
Authors: Pongprayoon Wasinee , Panya Atikorn , Jaresitthikunchai Janthima , Phaonakrop Narumon , Roytrakul Sittiruk
Published: October 2022

This study aims to identify novel chitosan (CTS)-responsive phosphoproteins in Leung Pratew 123 (LPT123) and Khao Dawk Mali 105 (KDML105) as drought-sensitive rice cultivars and differences in the CTS response. Rice seeds were soaked in CTS solution before germination, and 2- and 4-week-old rice seedlings sprayed with CTS before osmotic stress comprised the following four groups: (1) seedlings treated with distilled water; (2) seedlings treated with CTS; (3) seedlings pretreated with distilled water and subjected to osmotic stress; and (4) seedlings pretreated with CTS and subjected to osmotic stress. Phosphoproteins of leaf tissues were enriched using immobilized metal affinity chromatography (IMAC) before tryptic digestion and analysis via LC-MS. Phosphoprotein profiling analyses led to the identification of 4721 phosphoproteins representing 1052 and 1040 unique phosphoproteins in the LPT123 and KDML105 seedlings, respectively. In response to CTS pretreatment before osmotic stress, 22 differently expressed proteins were discovered, of which 10 and 12 were identified in the LPT123 and KDML105, respectively. These proteins are typically involved in signaling, transport, protein folding, protein degradation, and metabolism. This study provides fruitful data to understand the signal transduction mechanisms of rice seedlings pretreated with CTS before exposure to osmotic stress.
A Novel Seimatosporium and Other Sporocadaceae Species Associated with Grapevine Trunk Diseases in Cyprus
Authors: Kanetis Loukas I. , Taliadoros Demetris , Makris Georgios , Christoforou Michalis
Published: October 2022

Besides well-known grapevine trunk disease (GTD)-related pathogens, there is an increased interest in wood-colonizing fungi that infect grapevines. During 2017–2018, a survey was conducted in Cyprus and wood samples were collected from vines exhibiting typical GTD symptoms. Based on morphological and multilocus phylogenetic analyses (ITS, LSU, bt2, tef1-a), four species in the Sporocadaceae family were described and typified; two in the genus of Seimatosporium: Seim. cyprium sp. nov. and Seim. vitis-viniferae and two in Sporocadus: Spo. kurdistanicus and Spo. rosigena. The teleomorph of Seim. cyprium sp. nov. was also described. Pathogenicity trials with representative isolates of each species were performed on woody stems of two-year-old potted grapevines for 12 months under field conditions. All isolates were pathogenic, causing dark brown to black vascular discoloration, extending upward and downward from the inoculation point. Sporocadus isolates were significantly more aggressive than Seimatosporium with lesion lengths ranging from 9.24 to 6.90 and 4.13 to 4.00 cm, respectively. Successful re-isolations were also evident for all species and isolates. Seim. cyprium sp. nov. is a newly described species, while Spo. kurdistanicus and Spo. rosigena are reported for the first time in Europe on Vitis vinifera, suggesting the potential role of Sporocadaceae in the GTDs complex.
Characterization of Forage Quality, Phenolic Profiles, and Antioxidant Activity in Alfalfa (Medicago sativa L.)
Authors: Horvat Daniela , Viljevac Vuletić Marija , Andrić Luka , Baličević Renata , Kovačević Babić Marija , Tucak Marijana
Published: October 2022

Alfalfa (Medicago sativa L.) is one of the most important forage species and is recently more in focus for human consumption mainly due to its content of bioactive phenolics. Samples of the seventeen alfalfa cultivars/populations were collected at the Agricultural Institute Osijek with the aim to evaluate their forage quality, phenolic profiles, and antioxidant potential. Significant differences (p < 0.05) existed among studied alfalfa in all analyzed traits. The cultivar OS 99 and populations L7 and L20 were characterized by high crude protein content (22.5–24.7%) and the lowest neutral (40.2–42.9%) and acid detergent fibres (33–35.5%). The soluble-free phenolics from alfalfa leaves were extracted by methanol while insoluble cell-wall bound phenolics were released by alkaline hydrolysis. The bound phenolic extract showed a stronger DPPH scavenging capacity (20.8 mg TE/g dm) than the soluble (11.4 mg TE/g dm). The HPLC data revealed that more phenolics were found in the bound (3638.0 μg/g dm) than in the soluble form (912.3 μg/g dm). In the soluble extract of the alfalfa leaves, the major compound was catechin (338.3 μg/g dm), while rutin, epicatechin, and ferulic acid were minor ones. In the bound phenolic extract, the most abundant was ferulic (2198.2 μg/g dm) and p-coumaric acid (983.7 μg/g dm), followed by myricetin, apigenin, and quercetin. The principal component analysis revealed that alfalfa cultivars/populations were better discriminated based on the data on phenolics, rather than on forage quality. The cultivars/populations Florida 66, OS 66, L 40, L 42, Seed Force 4, and Torlesse were the most interesting in terms of phenolic health-promoting characteristics.