bluuebunny/crossref_metadata_2025 huggingface dataset:
Sense of Coherence Among Apprentices in Vocational Education and Training in Norway: Exploring General Resistance Resources in Work-Based Learning
Authors: Hanssen Grete , Utvær Britt Karin
Published: December 2022

Purpose: In the face of constant and rapid development achieving a work–life balance requires highly qualified and motivated professionals who can deal with stress and the demands of the future. In response, by facilitating school- and work-based learning, upper secondary vocational education and training (VET) prepares and qualifies adolescents for professional practice. In relation to VET, two fundamental salutogenic concepts can explain how humans cope with stressful and demanding situations: Sense of coherence (SOC), which entails comprehensibility, manageability and meaningfulness, and general resistance resources (GRRs), theorised as resources within individuals themselves (i.e., internal) and/or in their surroundings (i.e., external). Against that background, in our study we aimed to identify and explore which GRRs contribute to SOC among apprentices in VET and how they influence work-based learning. Methods: Data were collected from 11 VET student in Norway at three time points—at interviews during their final semester of school-based learning (i.e., spring 2020), at interviews during their work-based learning (i.e., spring 2022) and, for register data, following their completion of trade certificates (i.e., autumn 2022). The students' experiences of work-based learning as apprentices formed the basis of this study's analysis. The stepwise deductive–inductive method was used to generate and analyse the data. Findings: The apprentices' GRRs emerged in emotional, cognitive, physical, professional, social, relational, material and cultural dimensions. Identification and utilisation of GRRs were both individually and socially conditioned. The apprentices were able to identify, both consciously and unconsciously, internal GRRs to further identify external GRRs, and vice versa. Each identified GRR appeared to affect at least one, if not all, of the three components of SOC. Conclusion: Salutogenic VET can provide personal, social, environmental and physical resources (i.e., GRRs) that ensure coherent learning experiences. For that reason, by supporting VET students in identifying GRRs, teachers and supervisors can promote their SOC and work-based learning and thereby prepare them to be highly qualified and motivated professionals.
Multi-spectral SWIR lidar for imaging and spectral discrimination through partial obscurations
Authors: Sivaprakasam Vasanthi , Lin Di , Yetzbacher Michael K. , Gemar Heath E. , Portier Joel M. , Watnik Abbie T.
Published: December 2022

We have developed a multi-spectral SWIR lidar system capable of measuring simultaneous spatial-spectral information for imaging and spectral discrimination through partial obscurations. We employ objects in the presence and absence of a series of obscurants to evaluate the capability of the system in classifying the objects of interest based on spectral and range information. We employ a principal component analysis-based algorithm in classifying the objects and quantifying the accuracy of detection under various obscured scenarios. The merits of multi-spectral lidar over hyperspectral imaging are highlighted for target identification in the presence of obscurants.
Accurate and flexible calibration method for a 3D microscopic structured light system with telecentric imaging and Scheimpflug projection
Authors: Deng Huiwen , Hu Pengyu , Zhang Guofeng , Xia Chengsheng , Cai Yindi , Yang Shuming
Published: December 2022

3D imaging and metrology of complex micro-structures is a critical task for precision manufacturing and inspection. In this paper, an accurate and flexible calibration method for 3D microscopic structured light system with telecentric imaging and Scheimpflug projector is proposed. Firstly, a fringe projection 3D microscopy (FP-3DM) system consisting of a telecentric camera and a Scheimpflug projector is developed, which can take full advantage of the depth of field (DOF) and increase the measurement depth range. Secondly, an accurate and flexible joint calibration method is proposed to calibrate the developed system, which utilizes the established pinhole imaging model and Scheimpflug distortion model to calibrate telecentric imaging, and fully considers the correction and error optimization of the Scheimpflug projection model. Meanwhile, the optimized local homography is calculated to obtain more accurate sub-pixel correspondence between the camera and the projector, and the perspective-n-point (PnP) method make the 3D coordinate estimation of the feature point more accurate. Finally, a prototype and a dedicated calibration program are developed to realize high-resolution and high-precision 3D imaging. The experimental results demonstrate that the re-projection error is less than 1µm, and the 3D repeated measurement error based on feature fitting is less than 4µm, within the calibrated volume of 10(H)mm × 50(W)mm × 40(D)mm.
Fine-scale diversity of microbial communities due to satellite niches in boom and bust environments
Authors: Fridman Yulia , Wang Zihan , Maslov Sergei , Goyal Akshit
Published: December 2022

Recent observations have revealed that closely related strains of the same microbial species can stably coexist in natural and laboratory settings subject to boom and bust dynamics and serial dilutions, respectively. However, the possible mechanisms enabling the coexistence of only a handful of strains, but not more, have thus far remained unknown. Here, using a consumer-resource model of microbial ecosystems, we propose that by differentiating along Monod parameters characterizing microbial growth rates in high and low nutrient conditions, strains can coexist in patterns similar to those observed. In our model, boom and bust environments create satellite niches due to resource concentrations varying in time. These satellite niches can be occupied by closely related strains, thereby enabling their coexistence. We demonstrate that this result is valid even in complex environments consisting of multiple resources and species. In these complex communities, each species partitions resources differently and creates separate sets of satellite niches for their own strains. While there is no theoretical limit to the number of coexisting strains, in our simulations, we always find between 1 and 3 strains coexisting, consistent with known experiments and observations.
Bayesian inference of multi-point macromolecular architecture mixtures at nanometre resolution
Authors: Embacher Peter A. , Germanova Tsvetelina E. , Roscioli Emanuele , McAinsh Andrew D. , Burroughs Nigel J.
Published: December 2022

Gaussian spot fitting methods have significantly extended the spatial range where fluorescent microscopy can be used, with recent techniques approaching nanometre (nm) resolutions. However, small inter-fluorophore distances are systematically over-estimated for typical molecular scales. This bias can be corrected computationally, but current algorithms are limited to correcting distances between pairs of fluorophores. Here we present a flexible Bayesian computational approach that infers the distances and angles between multiple fluorophores and has several advantages over these previous methods. Specifically it improves confidence intervals for small lengths, estimates measurement errors of each fluorophore individually and infers the correlations between polygon lengths. The latter is essential for determining the full multi-fluorophore 3D architecture. We further developed the algorithm to infer the mixture composition of a heterogeneous population of multiple polygon states. We use our algorithm to analyse the 3D architecture of the human kinetochore, a macro-molecular complex that is essential for high fidelity chromosome segregation during cell division. Using triple fluorophore image data we unravel the mixture of kinetochore states during human mitosis, inferring the conformation of microtubule attached and unattached kinetochores and their proportions across mitosis. We demonstrate that the attachment conformation correlates with intersister tension and sister alignment to the metaphase plate.
Robust and brain-like working memory through short-term synaptic plasticity
Authors: Kozachkov Leo , Tauber John , Lundqvist Mikael , Brincat Scott L. , Slotine Jean-Jacques , Miller Earl K.
Published: December 2022

Working memory has long been thought to arise from sustained spiking/attractor dynamics. However, recent work has suggested that short-term synaptic plasticity (STSP) may help maintain attractor states over gaps in time with little or no spiking. To determine if STSP endows additional functional advantages, we trained artificial recurrent neural networks (RNNs) with and without STSP to perform an object working memory task. We found that RNNs with and without STSP were able to maintain memories despite distractors presented in the middle of the memory delay. However, RNNs with STSP showed activity that was similar to that seen in the cortex of a non-human primate (NHP) performing the same task. By contrast, RNNs without STSP showed activity that was less brain-like. Further, RNNs with STSP were more robust to network degradation than RNNs without STSP. These results show that STSP can not only help maintain working memories, it also makes neural networks more robust and brain-like.
Forward genetic screen of homeostatic antibody levels in the Collaborative Cross identifies MBD1 as a novel regulator of B cell homeostasis
Authors: Hampton Brea K. , Plante Kenneth S. , Whitmore Alan C. , Linnertz Colton L. , Madden Emily A. , Noll Kelsey E. , Boyson Samuel P. , Parotti Breantie , Xenakis James G. , Bell Timothy A. , Hock Pablo , Shaw Ginger D. , de Villena Fernando Pardo-Manuel , Ferris Martin T. , Heise Mark T.
Published: December 2022

Variation in immune homeostasis, the state in which the immune system is maintained in the absence of stimulation, is highly variable across populations. This variation is attributed to both genetic and environmental factors. However, the identity and function of specific regulators have been difficult to identify in humans. We evaluated homeostatic antibody levels in the serum of the Collaborative Cross (CC) mouse genetic reference population. We found heritable variation in all antibody isotypes and subtypes measured. We identified 4 quantitative trait loci (QTL) associated with 3 IgG subtypes: IgG1, IgG2b, and IgG2c. While 3 of these QTL map to genome regions of known immunological significance (major histocompatibility and immunoglobulin heavy chain locus), Qih1 (associated with variation in IgG1) mapped to a novel locus on Chromosome 18. We further associated this locus with B cell proportions in the spleen and identify Methyl-CpG binding domain protein 1 under this locus as a novel regulator of homeostatic IgG1 levels in the serum and marginal zone B cells (MZB) in the spleen, consistent with a role in MZB differentiation to antibody secreting cells.
Leveraging pleiotropy to discover and interpret GWAS results for sleep-associated traits
Authors: Chun Sung , Akle Sebastian , Teodosiadis Athanasios , Cade Brian E. , Wang Heming , Sofer Tamar , Evans Daniel S. , Stone Katie L. , Gharib Sina A. , Mukherjee Sutapa , Palmer Lyle J. , Hillman David , Rotter Jerome I. , Hanis Craig L. , Stamatoyannopoulos John A. , Redline Susan , Cotsapas Chris , Sunyaev Shamil R.
Published: December 2022

Genetic association studies of many heritable traits resulting from physiological testing often have modest sample sizes due to the cost and burden of the required phenotyping. This reduces statistical power and limits discovery of multiple genetic associations. We present a strategy to leverage pleiotropy between traits to both discover new loci and to provide mechanistic hypotheses of the underlying pathophysiology. Specifically, we combine a colocalization test with a locus-level test of pleiotropy. In simulations, we show that this approach is highly selective for identifying true pleiotropy driven by the same causative variant, thereby improves the chance to replicate the associations in underpowered validation cohorts and leads to higher interpretability. Here, as an exemplar, we use Obstructive Sleep Apnea (OSA), a common disorder diagnosed using overnight multi-channel physiological testing. We leverage pleiotropy with relevant cellular and cardio-metabolic phenotypes and gene expression traits to map new risk loci in an underpowered OSA GWAS. We identify several pleiotropic loci harboring suggestive associations to OSA and genome-wide significant associations to other traits, and show that their OSA association replicates in independent cohorts of diverse ancestries. By investigating pleiotropic loci, our strategy allows proposing new hypotheses about OSA pathobiology across many physiological layers. For example, we identify and replicate the pleiotropy across the plateletcrit, OSA and an eQTL of DNA primase subunit 1 (PRIM1) in immune cells. We find suggestive links between OSA, a measure of lung function (FEV1/FVC), and an eQTL of matrix metallopeptidase 15 (MMP15) in lung tissue. We also link a previously known genome-wide significant peak for OSA in the hexokinase 1 (HK1) locus to hematocrit and other red blood cell related traits. Thus, the analysis of pleiotropic associations has the potential to assemble diverse phenotypes into a chain of mechanistic hypotheses that provide insight into the pathogenesis of complex human diseases.
Tracking N- and C-termini of C. elegans polycystin-1 reveals their distinct targeting requirements and functions in cilia and extracellular vesicles
Authors: Walsh Jonathon D. , Wang Juan , DeHart Molly , Nikonorova Inna A. , Srinivasan Jagan , Barr Maureen M.
Published: December 2022

The cilium acts as an antenna receiving and sending signals, the latter via extracellular vesicles (EVs). In C. elegans and mammals, the Autosomal Dominant Polycystic Kidney Disease (ADPKD) gene products polycystin-1 (PC1) and polycystin-2 (PC2) localize to both cilia and EVs, act in the same genetic pathway, and function in a sensory capacity, suggesting ancient conservation. However, the functions of the polycystins on cilia and EVs remain enigmatic. We used our C. elegans model and endogenously fluorescent-tagged LOV-1/polycystin-1 to study LOV-1 processing, trafficking, transport, EV biogenesis, and function in living animals. Super resolution, real time imaging reveals that LOV-1 is processed into N-terminal (NTM) and C-terminal (CTM) forms via a conserved GPCR proteolytic site (GPS). The LOV-1 NTM is secreted into the extracellular matrix and not localized to ciliary tip EVs. In contrast, LOV-1 CTM and PKD-2 are co-trafficked, co-transported, and co-localized in cilia and on environmentally released ciliary EVs. LOV-1 CTM requires PKD-2 for ciliary EV localization, while PKD-2 localizes to ciliary EVs independent of LOV-1. We find that LOV-1 but not PKD-2 is required for chemosensation of an ascaroside mating pheromone. These findings indicate that the polycystins LOV-1 and PKD-2 function together and independently and provide insight to how cargo is selected and packaged in ciliary EVs.
Beneficial and detrimental genes in the cellular response to replication arrest
Authors: Schons-Fonseca Luciane , Lazova Milena D. , Smith Janet L. , Anderson Mary E. , Grossman Alan D.
Published: December 2022

DNA replication is essential for all living organisms. Several events can disrupt replication, including DNA damage (e.g., pyrimidine dimers, crosslinking) and so-called “roadblocks” (e.g., DNA-binding proteins or transcription). Bacteria have several well-characterized mechanisms for repairing damaged DNA and then restoring functional replication forks. However, little is known about the repair of stalled or arrested replication forks in the absence of chemical alterations to DNA. Using a library of random transposon insertions inBacillus subtilis, we identified 35 genes that affect the ability of cells to survive exposure to an inhibitor that arrests replication elongation, but does not cause chemical alteration of the DNA. Genes identified include those involved in iron-sulfur homeostasis, cell envelope biogenesis, and DNA repair and recombination. InB.subtilis, and many bacteria, two nucleases (AddAB and RecJ) are involved in early steps in repairing replication forks arrested by chemical damage to DNA and loss of either nuclease causes increased sensitivity to DNA damaging agents. These nucleases resect DNA ends, leading to assembly of the recombinase RecA onto the single-stranded DNA. Notably, we found that disruption ofrecJincreased survival of cells following replication arrest, indicating that in the absence of chemical damage to DNA, RecJ is detrimental to survival. In contrast, and as expected, disruption ofaddAdecreased survival of cells following replication arrest, indicating that AddA promotes survival. The different phenotypes ofaddAandrecJmutants appeared to be due to differences in assembly of RecA onto DNA. RecJ appeared to promote too much assembly of RecA filaments. Our results indicate that in the absence of chemical damage to DNA, RecA is dispensable for cells to survive replication arrest and that the stable RecA nucleofilaments favored by the RecJ pathway may lead to cell death by preventing proper processing of the arrested replication fork.
Stress combined with loss of the Candida albicans SUMO protease Ulp2 triggers selection of aneuploidy via a two-step process
Authors: Rizzo Marzia , Soisangwan Natthapon , Vega-Estevez Samuel , Price Robert Jordan , Uyl Chloe , Iracane Elise , Shaw Matt , Soetaert Jan , Selmecki Anna , Buscaino Alessia
Published: December 2022

A delicate balance between genome stability and instability ensures genome integrity while generating genetic diversity, a critical step for evolution. Indeed, while excessive genome instability is harmful, moderated genome instability can drive adaptation to novel environments by maximising genetic variation.Candida albicans, a human fungal pathogen that colonises different parts of the human body, adapts rapidly and frequently to different hostile host microenvironments. In this organism, the ability to generate large-scale genomic variation is a key adaptative mechanism triggering dangerous infections even in the presence of antifungal drugs. Understanding how fitter novel karyotypes are selected is key to determining howC.albicansand other microbial pathogens establish infections. Here, we identified the SUMO protease Ulp2 as a regulator ofC.albicansgenome integrity through genetic screening. Deletion ofULP2leads to increased genome instability, enhanced genome variation and reduced fitness in the absence of additional stress. The combined stress caused by the lack ofULP2and antifungal drug treatment leads to the selection of adaptive segmental aneuploidies that partially rescue the fitness defects ofulp2Δ/Δ cells. Short and long-read genomic sequencing demonstrates that these novel genotypes are selected via a two-step process leading to the formation of novel chromosomal fragments with breakpoints at microhomology regions and DNA repeats.
“I see salt everywhere”: A qualitative examination of the utility of arts-based participatory workshops to study noncommunicable diseases in Tanzania and Malawi
Authors: Bissett Maria , Gray Cindy M. , Abdulla Sharifa , Bunn Christopher , Crampin Amelia C. , Dillip Angel , Gill Jason M. R. , Kaare Heri C. , Kalima Sharon , Kambalu Elson , Lwanda John , Makoye Herbert F. , Mtema Otiyela , Perry Mia , Strachan Zoë , Todd Helen , Mtenga Sally M.
Published: December 2022

The burden of noncommunicable diseases (NCDs) including hypertension, diabetes, and cancer, is rising in Sub-Saharan African countries like Tanzania and Malawi. This increase reflects complex interactions between diverse social, environmental, biological, and political factors. To intervene successfully, new approaches are therefore needed to understand how local knowledges and attitudes towards common NCDs influence health behaviours. This study compares the utility of using a novel arts-based participatory method and more traditional focus groups to generate new understandings of local knowledges, attitudes, and behaviours towards NCDs and their risk factors. Single-gender arts-based participatory workshops and focus group discussions were conducted with local communities in Tanzania and Malawi. Thematic analysis compared workshop and focus group transcripts for depth of content and researcher-participant hierarchies. In addition, semiotic analysis examined the contribution of photographs of workshop activities to understanding participants’ experiences and beliefs about NCD risk factors. The arts-based participatory workshops produced in-depth, vivid, emotive narratives of participants’ beliefs about NCDs and their impact (e.g., “… it spreads all over your body and kills you—snake’s poison is similar to diabetes poison”), while the focus groups provided more basic accounts (e.g., “diabetes is a fast killer”). The workshops also empowered participants to navigate activities with autonomy, revealing their almost overwhelmingly negative beliefs about NCDs. However, enabling participants to direct the focus of workshop activities led to challenges, including the perpetuation of stigma (e.g., comparing smells associated with diabetes symptoms with sewage). Semiotic analysis of workshop photographs provided little additional insight beyond that gained from the transcripts. Arts-based participatory workshops are promising as a novel method to inform development of culturally relevant approaches to NCD prevention in Tanzania and Malawi. Future research should incorporate more structured opportunities for participant reflection during the workshops to minimise harm from any emerging stigma.
Geographic barriers to care persist at the community healthcare level: Evidence from rural Madagascar
Authors: Evans Michelle V. , Andréambeloson Tanjona , Randriamihaja Mauricianot , Ihantamalala Felana , Cordier Laura , Cowley Giovanna , Finnegan Karen , Hanitriniaina Feno , Miller Ann C. , Ralantomalala Lanto Marovavy , Randriamahasoa Andry , Razafinjato Bénédicte , Razanahanitriniaina Emeline , Rakotonanahary Rado J. L. , Andriamiandra Isaïe Jules , Bonds Matthew H. , Garchitorena Andres
Published: December 2022

Geographic distance is a critical barrier to healthcare access, particularly for rural communities with poor transportation infrastructure who rely on non-motorized transportation. There is broad consensus on the importance of community health workers (CHWs) to reduce the effects of geographic isolation on healthcare access. Due to a lack of fine-scale spatial data and individual patient records, little is known about the precise effects of CHWs on removing geographic barriers at this level of the healthcare system. Relying on a high-quality, crowd-sourced dataset that includes all paths and buildings in the area, we explored the impact of geographic distance from CHWs on the use of CHW services for children under 5 years in the rural district of Ifanadiana, southeastern Madagascar from 2018–2021. We then used this analysis to determine key features of an optimal geographic design of the CHW system, specifically optimizing a single CHW location or installing additional CHW sites. We found that consultation rates by CHWs decreased with increasing distance patients travel to the CHW by approximately 28.1% per km. The optimization exercise revealed that the majority of CHW sites (50/80) were already in an optimal location or shared an optimal location with a primary health clinic. Relocating the remaining CHW sites based on a geographic optimum was predicted to increase consultation rates by only 7.4%. On the other hand, adding a second CHW site was predicted to increase consultation rates by 31.5%, with a larger effect in more geographically dispersed catchments. Geographic distance remains a barrier at the level of the CHW, but optimizing CHW site location based on geography alone will not result in large gains in consultation rates. Rather, alternative strategies, such as the creation of additional CHW sites or the implementation of proactive care, should be considered.
Cost of wastewater-based environmental surveillance for SARS-CoV-2: Evidence from pilot sites in Blantyre, Malawi and Kathmandu, Nepal
Authors: Ngwira Lucky G. , Sharma Bhawana , Shrestha Kabita Bade , Dahal Sushil , Tuladhar Reshma , Manthalu Gerald , Chilima Ben , Ganizani Allone , Rigby Jonathan , Kanjerwa Oscar , Barnes Kayla , Anscombe Catherine , Mfutso-Bengo Joseph , Feasey Nicholas , Mvundura Mercy
Published: December 2022

Environmental surveillance of rivers and wastewater for SARS-CoV-2 detection has been explored as an innovative way to surveil the pandemic. This study estimated the economic costs of conducting wastewater-based environmental surveillance for SARS-CoV-2 to inform decision making if countries consider continuing these efforts. We estimated the cost of two SARS-CoV-2 environmental surveillance pilot studies conducted in Blantyre, Malawi, and Kathmandu, Nepal. The cost estimation accounted for the consumables, equipment, and human resource time costs used for environmental surveillance from sample selection until pathogen detection and overhead costs for the projects. Costs are reported in 2021 US$ and reported as costs per month, per sample and person per year. The estimated costs for environmental surveillance range from $6,175 to $8,272 per month (Blantyre site) and $16,756 to $30,050 (Kathmandu site). The number of samples processed per month ranged from 84 to 336 at the Blantyre site and 96 to 250 at the Kathmandu site. Consumables costs are variable costs influenced by the number of samples processed and are a large share of the monthly costs for ES (ranging from 39% to 72%). The relatively higher costs per month for the Kathmandu site were attributable to the higher allocation of dedicated human resources and equipment to environmental surveillance for SARS-CoV-2 compared to the Blantyre site where these resources were shared with other activities. The average cost per sample ranged from $25 to $74 (Blantyre) and $120 to $175 (Kathmandu). There were associated economies of scale for human resources and equipment costs with increased sample processing and sharing of resources with other activities. The cost per person in the catchment area per year ranged from $0.07 to $0.10 in Blantyre and $0.07 to $0.13 in Kathmandu. Environmental surveillance may be a low-cost early warning signal for SARS-CoV-2 that can complement other SARS-CoV2 monitoring efforts.
The effect of population-based blood pressure screening on long-term cardiometabolic morbidity and mortality in Germany: A regression discontinuity analysis
Authors: Pedron Sara , Hanselmann Michael , Burns Jacob , Rich Alexander , Peters Annette , Heier Margit , Schwettmann Lars , Bor Jacob H. , Bärnighausen Till , Laxy Michael
Published: December 2022

Background

Hypertension represents one of the major risk factors for cardiovascular morbidity and mortality globally. Early detection and treatment of this condition is vital to prevent complications. However, hypertension often goes undetected, and even if detected, not every patient receives adequate treatment. Identifying simple and effective interventions is therefore crucial to fight this problem and allow more patients to receive the treatment they need. Therefore, we aim at investigating the impact of a population-based blood pressure (BP) screening and the subsequent “low-threshold” information treatment on long-term cardiovascular disease (CVD) morbidity and mortality.

Methods and findings

We examined the impact of a BP screening embedded in a population-based cohort study in Germany and subsequent personalized “light touch” information treatment, including a hypertension diagnosis and a recommendation to seek medical attention. We pooled four waves of the KORA study, carried out between 1984 and 1996 (N = 14,592). Using a sharp multivariate regression discontinuity (RD) design, we estimated the impact of the information treatment on CVD mortality and morbidity over 16.9 years. Additionally, we investigated potential intermediate outcomes, such as hypertension awareness, BP, and behavior after 7 years.

No evidence of effect of BP screening was observed on CVD mortality (hazard ratio (HR) = 1.172 [95% confidence interval (CI): 0.725, 1.896]) or on any (fatal or nonfatal) long-term CVD event (HR = 1.022 [0.636, 1.641]) for individuals just above (versus below) the threshold for hypertension. Stratification for previous self-reported diagnosis of hypertension at baseline did not reveal any differential effect. The intermediate outcomes, including awareness of hypertension, were also unaffected by the information treatment. However, these results should be interpreted with caution since the analysis might not be sufficiently powered to detect a potential intervention effect.

Conclusions

The study does not provide evidence of an effect of the assessed BP screening and subsequent information treatment on BP, health behavior, or long-term CVD mortality and morbidity. Future studies should consider larger datasets to detect possible effects and a shorter follow-up for the intermediate outcomes (i.e., BP and behavior) to detect short-, medium-, and long-term effects of the intervention along the causal pathway.
The effectiveness of prosocial policies: Gender differences arising from social norms
Authors: Cabrales Antonio , Kendall Ryan , Sánchez Angel
Published: December 2022

We study policies aimed at discouraging behavior that produces negative externalities, and their differential gender impact. Using driving as an application, we carry out an experiment where slowest vehicles are the safest choice, whereas faster driving speeds lead to higher potential payoffs but higher probabilities of accidents. Faster speeds have a personal benefit but create a negative externality. We consider four experimental policy conditions: a baseline situation, a framing condition in which drivers are suggested that driving fast violates a social norm, and two punishment conditions, one exogenous and one endogenous. We find that the most effective policies use different framing and endogenously determined punishment mechanisms (to fast drivers by other drivers). These policies are only effective for female drivers which leads to substantial gender payoff differences. Our data suggest that these results arise from differences in social norms across genders, thus opening the way to designing more effective policies.
Systematic development and feasibility testing of a multibehavioural digital prehabilitation intervention for patients approaching major surgery (iPREPWELL): A study protocol
Authors: Durrand J. , Livingston R. , Tew G. , Gillis C. , Yates D. , Gray J. , Greaves C. , Moore J. , O’Doherty A. F. , Doherty P. , Danjoux G. , Avery L.
Published: December 2022

Improving outcomes for people undergoing major surgery, specifically reducing perioperative morbidity and mortality remains a global health challenge. Prehabilitation involves the active preparation of patients prior to surgery, including support to tackle risk behaviours that mediate and undermine physical and mental health and wellbeing. The majority of prehabilitation interventions are delivered in person, however many patients express a preference for remotely-delivered interventions that provide them with tailored support and the flexibility. Digital prehabilitation interventions offer scalability and have the potential to benefit perioperative healthcare systems, however there is a lack of robustly developed and evaluated digital programmes for use in routine clinical care. We aim to systematically develop and test the feasibility of an evidence and theory-informed multibehavioural digital prehabilitation intervention ‘iPREPWELL’ designed to prepare patients for major surgery. The intervention will be developed with reference to the Behaviour Change Wheel, COM-B model, and the Theoretical Domains Framework. Codesign methodology will be used to develop a patient intervention and accompanying training intervention for healthcare professionals. Training will be designed to enable healthcare professionals to promote, support and facilitate delivery of the intervention as part of routine clinical care. Patients preparing for major surgery and healthcare professionals involved with their clinical care from two UK National Health Service centres will be recruited to stage 1 (systematic development) and stage 2 (feasibility testing of the intervention). Participants recruited at stage 1 will be asked to complete a COM-B questionnaire and to take part in a qualitative interview study and co-design workshops. Participants recruited at stage 2 (up to twenty healthcare professionals and forty participants) will be asked to take part in a single group intervention study where the primary outcomes will include feasibility, acceptability, and fidelity of intervention delivery, receipt, and enactment. Healthcare professionals will be trained to promote and support use of the intervention by patients, and the training intervention will be evaluated qualitatively and quantitatively. The multifaceted and systematically developed intervention will be the first of its kind and will provide a foundation for further refinement prior to formal efficacy testing.
The association of herd performance indicators with dairy cow longevity: An empirical study
Authors: Han Ruozhu , Mourits Monique , Steeneveld Wilma , Hogeveen Henk
Published: December 2022

The associations between reproductive performance, milk yield and health status with the risk of culling, and thus with a cow’s longevity, have been well documented at the individual cow level. Associations at individual cow level may, however, not be valid at herd level due to interrelated herd management aspects and/or policy restrictions. The objective of this study was to explore the association of herd performance indicators with herd-level dairy cow longevity under Dutch production conditions. Longevity was expressed by three different measures, viz. age at culling, lifetime milk production of culled cows and culling rate. The evaluated herd performance indicators included factors on milk production, youngstock rearing, reproduction and health performance as registered on 10 719 Dutch commercial dairy herds during the period 2007–2016. Averaged over herds and the evaluated period, the age of culled milking cows was 2 139 days (5.8 years, SD±298 days), the lifetime milk production of culled cows was 31 238 kg (SD±7,494 kg), and the culling rate was 0.24 (SD±0.08). A mixed linear regression modelling approach was applied to evaluate the association of each of the three longevity measures with the selected herd performance indicators. The results indicated that only four herd performance indictors (herd size, herd expansion, heifer ratio and the proportion of cows with potential subclinical ketosis) shared significant associations with all three longevity variables. Generally, the strength of the associations between each of the evaluated longevity measures and herd performance indicators was only limited. The absence of strong associations between the longevity measures and herd performance indicators reveal that there is potential of extending cattle longevity without affecting the herd performance in terms of milk production, reproduction and health. Moreover, only part of the observed variance in longevity among the herds over time was explained by the herd performance variables, indicating that differences in longevity at herd level may predominantly be determined by other factors, like farmers’ attitude and strategic management.
Sonodynamic therapy suppresses matrix collagen degradation in vulnerable atherosclerotic plaque by modulating caspase 3 - PEDF/HIF-1α - MMP-2/MMP-9 signaling in macrophages
Authors: Tian Yanfeng , Sheng Siqi , Gao Weiwei , Yao Jianting , Tian Ye
Published: December 2022

Background

The rupture of vulnerable atherosclerotic plaque is the main cause of acute ischemic vascular events, and is characterized by pathological degradation of matrix collagen in the fibrous cap. In a previous study, we reported that 5-aminolevulinic acid-mediated sonodynamic therapy suppressed collagen degradation in rabbit plaque. However, the underlying molecular mechanism has yet to be fully elucidated.

Methods

We applied sinoporphyrin sodium-mediated sonodynamic therapy (DVDMS-SDT) to balloon-denuded rabbit and apolipoprotein E-deficient (ApoE−/−) mouse models to observe collagen content in plaque. Cultured human THP-1 and mouse peritoneal macrophage-derived foam cells were used for in vitro mechanistic studies.

Results

We observed that DVDMS-SDT decreased plaque area and increased the percentages of collagen and smooth muscle cells and reduced the percentage of macrophages in rabbit and ApoE-/- mouse advanced plaques. In vitro, DVDMS-SDT modulated the caspase 3-pigment epithelium-derived factor/hypoxia-inducible factor-1α (PEDF/HIF-1α)-matrix metalloprotease-2/9 (MMP-2/MMP-9) signaling in macrophage foam cells.

Conclusions

Our findings show that DVDMS-SDT effectively inhibits matrix collagen degradation in advanced atherosclerotic plaque by modulating caspase 3-PEDF/HIF-1α-MMP-2/MMP-9 signaling in macrophage foam cells and therefore represents a suitable and promising clinical regimen to stabilize vulnerable plaques.
The effect of observation angles on facial age perceptions: A case study of Japanese women
Authors: Kurosumi Motonori , Mizukoshi Koji , Hongo Maya , Kamachi Miyuki G.
Published: December 2022

Most conventional aging research has limited its approach concerning the head and face shape and skin condition to the frontal face. However, in our daily lives, we observe facial features from various angles, which may reveal or obscure aging features that could only be identified under limited conditions in the past. This study systematically investigates the effect of facial observation angles—specifically, of horizontal and vertical angles—on age impression. A total of 112 Japanese women aged 20–49 years participated as observers who evaluated the age impressions of 280 Japanese women aged 20–69 years. A two-way analysis of the variance of the age impression score was conducted for two factors: observation angle (five angles with yaw and pitch directions) and age group (five ages, from the 20s to the 60s). The results reveal that, as compared with frontal observation, the perceived age tended to decrease with the facial observation angles and that the effect of the angle on perceived age decreased with increasing age, especially for the profile face. Understanding the effect of the facial observation angle on age impression and clarifying the characteristics of the face and skin not perceived in the frontal face will provide useful knowledge to make people look youthful, look more beautiful, and be happier in all aspects of their lives.
Sequential control of myeloid cell proliferation and differentiation by cytokine receptor-based chimeric antigen receptors
Authors: Nakajima Kyoko , Shen Zhongchuzi , Miura Masashi , Nakabayashi Hideto , Kawahara Masahiro
Published: December 2022

As chimeric antigen receptor (CAR)-T cell therapy has been recently applied in clinics, controlling the fate of blood cells is increasingly important for curing blood disorders. In this study, we aim to construct proliferation-inducing and differentiation-inducing CARs (piCAR and diCAR) with two different antigen specificities and express them simultaneously on the cell surface. Since the two antigens are non-cross-reactive and exclusively activate piCAR or diCAR, sequential induction from cell proliferation to differentiation could be controlled by switching the antigens added in the culture medium. To demonstrate this notion, a murine myeloid progenitor cell line 32Dcl3, which proliferates in an IL-3-dependent manner and differentiates into granulocytes when cultured in the presence of G-CSF, is chosen as a model. To mimic the cell fate control of 32Dcl3 cells, IL-3R-based piCAR and G-CSFR-based diCAR are rationally designed and co-expressed in 32Dcl3 cells to evaluate the proliferation- and differentiation-inducing functions. Consequently, the sequential induction from proliferation to differentiation with switching the cytokine from IL-3 to G-CSF is successfully replaced by switching the antigen from one to another in the CARs-co-expressing cells. Thus, piCAR and diCAR may become a platform technology for sequentially controlling proliferation and differentiation of various cell types that need to be produced in cell and gene therapies.
Career choice of tourism students in a triple-whammy crisis
Authors: Choy Monica Waichun , Yeung Alexander Seeshing
Published: December 2022

Hong Kong hospitality and tourism industry has been battered by the triple whammy of social unrest, Sino-US trade war and COVID-19 pandemic in recent years. To understand how vulnerable tourism students may be in terms of career shock when facing the three major challenges, 407 tourism students in Hong Kong were surveyed. Structural equation modelling found a positive correlation between affect (an intrinsic, motivating factor) and extraneous events (an extrinsic, demotivating factor), indicating that motivation and demotivating factors may co-exist. Affect was more positively correlated with three career choice outcomes (intent to join the industry, desire for a lifelong career, and resilience in face of unfavourable circumstances) than was Extraneous. In face of career shock arising from extraneous events, tourism students still tend to have a strong intent to join the workforce, take it as a lifelong career, and remain resilient despite the hardship. However, the career shock was a greater concern for those in hotel-related disciplines and for students aged over 20 than younger ones. The findings offer an empirical basis to guide policy makers, academia and the industry in strategy formulation to ensure sustainable quality and manpower supply in the post-crisis future.
Association of zinc deficiency with infectious complications in pediatric hematopoietic stem cell transplantation patients
Authors: Suwanphoerung Warangkhana , Klinmalai Chompunuch , Rattanasiri Sasivimol , Pakakasama Samart , Anurathapan Usanarat , Hongeng Suradej , Chongviriyaphan Nalinee , Apiwattanakul Nopporn
Published: December 2022

Background

Zinc plays essential roles in immune function and epithelial integrity. Patients undergoing hematopoietic stem cell transplantation (HSCT) often have low plasma zinc levels because of poor intake and diarrhea. We hypothesized that patients with zinc deficiency before HSCT had worse infectious complications after HSCT compared with patients with normal zinc levels. Citrulline, a marker of intestinal integrity, was also hypothesized to be lower in patients with zinc deficiency.

Patients and methods

Thirty patients undergoing HSCT at Ramathibodi Hospital during March 2020–September 2021 were enrolled. Blood samples for plasma zinc and citrulline were collected during the HSCT period. The 14- and 90-day outcomes after HSCT were prospectively recorded.

Results

Twelve of 30 (40%) patients had zinc deficiency before HSCT. Zinc-deficient patients were younger (median (interquartile range): 6 (8.8) vs 13 (5.8) years old; p = 0.017). Zinc levels tended to increase after admission in both groups. Patients with zinc deficiency had lower citrulline levels than those with normal zinc levels. Citrulline levels decreased in both groups after stem cell infusion, and the level was not significantly different between the two groups. Zinc-deficient patients had a higher rate of bacterial infection within 90 days after HSCT than those with normal zinc levels (6 in 12 patients (50.0%) vs 1 in 18 patients (5.6%); odds ratio [OR]: 17.0; 95% confidence interval [CI]: 1.68–171.70; p = 0.016). This remained significant after adjustments for age (adjusted OR: 12.31; 95% CI: 1.084–139.92; p = 0.043).

Conclusion

The prevalence of zinc deficiency in pediatric patients undergoing HSCT was high. Zinc-deficient patients had lower citrulline levels and higher incidence of bacterial infection after HSCT. However, citrulline level was not different between patients with and without bacterial infections. It is worth to investigate whether zinc supplementation before HSCT can reduce bacterial infection after HSCT.
Evolutionary game analysis of low-carbon technology innovation diffusion under PPP mode in China
Authors: Jia Chong , Zhang Ruixue , Wang Dan
Published: December 2022

With the Public–Private Partnership (PPP) mode playing an increasingly important role in the investment of infrastructure, promoting the diffusion of low-carbon technology innovation in PPP projects not only helps to reduce the carbon emissions of infrastructure, but also plays an important demonstration role in the low-carbon transformation of construction industry. In order to clarify the evolutionary mechanism of low-carbon technology innovation diffusion among stakeholders under the unique payment mechanism of PPP projects in China, the paper builds two different evolutionary game models respectively under government payment mechanism and consumer payment mechanism based on evolutionary game theory. The evolutionary relationship between the choice of behavior strategies and the change of influencing factors under different payment mechanisms are analyzed by numerical simulation. It is found that under government payment mechanism, the regulation cost of government to promote low-carbon technology innovation and the punishment of the superior regulatory authority for non-regulation behavior are the important factors affecting government’s behavioral strategies. The low-carbon technology innovation cost of social capital and the intensity of government subsidy and punishment are the main factors affecting social capital’s behavioral strategies; Under consumer payment mechanism, consumer becomes the ultimate payer of low-carbon products, and the income they get from purchasing low-carbon products and the subsidy provided by the government become the main factors determining consumer’s behavioral strategies. Finally, from the perspective of government, social capital and consumer, countermeasures and management implications are put forward to effectively promote the diffusion of low-carbon technology innovation under different payment mechanism in PPP projects.