bluuebunny/crossref_metadata_2025 huggingface dataset:
Advances in the Approximation of the Matrix Hyperbolic Tangent
Authors: Ibáñez Javier , Alonso José M. , Sastre Jorge , Defez Emilio , Alonso-Jordá Pedro
Published: May 2021

In this paper, we introduce two approaches to compute the matrix hyperbolic tangent. While one of them is based on its own definition and uses the matrix exponential, the other one is focused on the expansion of its Taylor series. For this second approximation, we analyse two different alternatives to evaluate the corresponding matrix polynomials. This resulted in three stable and accurate codes, which we implemented in MATLAB and numerically and computationally compared by means of a battery of tests composed of distinct state-of-the-art matrices. Our results show that the Taylor series-based methods were more accurate, although somewhat more computationally expensive, compared with the approach based on the exponential matrix. To avoid this drawback, we propose the use of a set of formulas that allows us to evaluate polynomials in a more efficient way compared with that of the traditional Paterson–Stockmeyer method, thus, substantially reducing the number of matrix products (practically equal in number to the approach based on the matrix exponential), without penalising the accuracy of the result.
Studying the Effect of Noise on Pricing and Marketing Decisions of New Products under Co-op Advertising Strategy in Supply Chains: Game Theoretical Approaches
Authors: Taleizadeh Ata Allah , Cheraghi Zahedeh , Cárdenas-Barrón Leopoldo Eduardo , Noori-Daryan Mahsa
Published: May 2021

The success of launching new products is the main challenge of companies since it is one of the key factors of competition. Thus, success in today’s high rival markets depends on the presentation of new products with new options, which must be compatible with customers’ desires. This research aims to analyze the psychological effect of the noise of a new product on the total profit of the chain and the optimal pricing and marketing decisions of the chain’s members. Additionally, a cooperative (co-op) advertising strategy as a coordination mechanism is considered among the partners such that it helps them to obtain their target markets. Commonly, under co-op advertising, the manufacturer pays a percentage of the retailer’s advertising costs. In this chain, the manufacturer and the retailer agree to share the retailer’s advertising costs. Afterwards, four different relations between the manufacturer and retailer are studied and analyzed including three non-cooperative games with symmetrical distribution of market power and one asymmetrical distribution of it. So, four game models and their closed-form solutions are illustrated with a numerical example. It was found that the noise effect affects the total profit of the manufacturer and the retailer, as well as the supply chain by influencing the partners’ advertising policies. In other word, increasing the noise effect of the product indicates to the manufacturer and the retailer to globally and locally advertise more, respectively. In turn, their profits increase, although also increasing the advertising costs. Finally, a complete sensitivity analysis is conducted and reported.
Variability in Macro- and Micronutrients of 15 Commercially Available Microalgae Powders
Authors: Sandgruber Fabian , Gielsdorf Annekathrin , Baur Anja C. , Schenz Benjamin , Müller Sandra Marie , Schwerdtle Tanja , Stangl Gabriele I. , Griehl Carola , Lorkowski Stefan , Dawczynski Christine
Published: May 2021

The nutrient composition of 15 commercially available microalgae powders of Arthrospira platensis, Chlorella pyrenoidosa and vulgaris, Dunaliella salina, Haematococcus pluvialis, Tetraselmis chuii, and Aphanizomenon flos-aquae was analyzed. The Dunaliella salina powders were characterized by a high content of carbohydrates, saturated fatty acids (SFAs), omega-6-polyunsaturated fatty acids (n6-PUFAs), heavy metals, and α-tocopherol, whereas the protein amounts, essential amino acids (EAAs), omega-3-PUFAs (n3-PUFAs), vitamins, and minerals were low. In the powder of Haematococcus pluvialis, ten times higher amounts of carotenoids compared to all other analyzed powders were determined, yet it was low in vitamins D and E, protein, and EAAs, and the n6/n3-PUFAs ratio was comparably high. Vitamin B12, quantified as cobalamin, was below 0.02 mg/100 g dry weight (d.w.) in all studied powders. Based on our analysis, microalgae such as Aphanizomenon and Chlorella may contribute to an adequate intake of critical nutrients such as protein with a high content of EAAs, dietary fibers, n3-PUFAs, Ca, Fe, Mg, and Zn, as well as vitamin D and E. Yet, the nutritional value of Aphanizomenon flos-aquae was slightly decreased by high contents of SFAs. The present data show that microalgae are rich in valuable nutrients, but the macro- and micronutrient profiles differ strongly between and within species.
Laboratory Study of the Ultraviolet Radiation Effect on an HDPE Geomembrane
Authors: Lavoie Fernando Luiz , Kobelnik Marcelo , Valentin Clever Aparecido , Tirelli Érica Fernanda da Silva , Lopes Maria de Lurdes , Silva Jefferson Lins da
Published: May 2021

High-density polyethylene (HDPE) geomembranes are polymeric geosynthetic materials usually applied as a liner in environmental facilities due to their good mechanical properties, good welding conditions, and excellent chemical resistance. A geomembrane’s field performance is affected by different conditions and exposures, including ultraviolet radiation, thermal and oxidative exposure, and chemical contact. This article presents an experimental study with a 1.0 mm-thick HDPE virgin geomembrane exposed by the Xenon arc weatherometer for 2160 h and the ultraviolet fluorescent weatherometer for 8760 h to understand the geomembrane’s behavior under ultraviolet exposure. The evaluation was performed using the melt flow index (MFI) test, oxidative-induction time (OIT) tests, tensile test, differential scanning calorimetry (DSC) analysis, and Fourier transform infrared spectroscopy (FTIR) analysis. The sample exposed in the Xenon arc equipment showed a tendency to increase the MFI values during the exposure time. This upward trend may indicate morphological changes in the polymer. The tensile behavior analysis showed a tendency of the sample to lose ductility, without showing brittle behavior. The samples’ OIT test results under both device exposures showed faster antioxidant depletion for the standard OIT test than the high-pressure OIT test. The DSC and FTIR analyses did not demonstrate the polymer’s changes.
Effects of Fe+2 and Fe+3 in Pretreatment and Leaching on a Mixed Copper Ore in Chloride Media
Authors: Taboada María E. , Hernández Pía C. , Padilla Aldo P. , Jamett Nathalie E. , Graber Teófilo A.
Published: May 2021

A study of the pretreatment stage and subsequent leaching of a mixed copper ore with different chloride solutions containing iron was carried out. The first stage considered pretreatment tests to decide the best conditions. Two levels of each factor were analyzed, 20 and 50 kg/t of NaCl, 17 and 25 kg/t of H2SO4, 0 and 25 kg/t of Fe2(SO4)3·9.2H2O, 0 and 25 kg/t of Fe2SO4·7H2O, and a curing time of 15 and 30 days. The results showed a significant effect of NaCl and curing time on the extraction, and less effect was found with the variation of acid and iron salts. The second stage included column leaching using a solution with 0.5 g/L of Cu+2, 80 g/L of Cl−, 10 g/L of H2SO4, and variable concentrations of ferric and ferrous ions (0 and 2 g/L). The best copper extraction of 80.2% was found considering a pretreatment of 30 days, 25 kg/t of H2SO4, 50 kg/t of NaCl, and a leaching solution concentration described previously with 2 g/L of Fe+2. The results showed the leaching of all copper oxide species and 20% of the copper sulfide species. In addition, there was a reduction in the acid consumption as the resting time increases. Furthermore, to evaluate a possible decrease in time and acid in pretreatment and chloride in leaching, tests including 10 and 25 kg/t of H2SO4 and 1, 15, and 30 days of curing and a diminution of the NaCl concentration to 20 g/L (content from seawater) were executed. The results showed a significant effect on curing time below 15 days. Furthermore, the slight influence of the decrease of acid on copper extraction gives cost reduction opportunities. The diminution of chloride concentration (80 to 20 g/L) in leaching solution decreases the extraction from 79% to 66.5%. Finally, the Mellado leaching kinetic model was successfully implemented.
Carbide Nanoparticle Dispersion Techniques for Metal Powder Metallurgy
Authors: Rocky Bahrum Prang , Weinberger Christopher R. , Daniewicz Steven R. , Thompson Gregory B.
Published: May 2021

Nanoparticles (NP) embedded into a matrix material have been shown to improve mechanical properties such as strength, hardness, and wear-resistance. However, the tendency of NPs to agglomerate in the powder mixing process is a major concern. This study investigates five different mechanochemical processing (MCP) routes to mitigate agglomeration to achieve a uniform dispersion of ZrC NPs in an Fe-based metal matrix composite. Our results suggest that MCP with only process controlling agents is ineffective in avoiding aggregation of these NPs. Instead, the uniformity of the carbide NP dispersion is achieved by pre-dispersing the NPs under ultrasonication using suitable surfactants followed by mechanically mixing of the NPs with iron powders in an alcohol solvent which is then dried. High-energy MCP is then used to embed the NPs within the powders. These collective steps resulted in a uniform dispersion of ZrC in the sintered (consolidated) Fe sample.
Investigation of the Thickness Differential on the Formability of Aluminum Tailor Welded Blanks
Authors: Wu Jie , Hovanski Yuri , Miles Michael
Published: May 2021

A finite element model is proposed to investigate the effect of thickness differential on Limiting Dome Height (LDH) testing of aluminum tailor-welded blanks. The numerical model is validated via comparison of the equivalent plastic strain and displacement distribution between the simulation results and the experimental data. The normalized equivalent plastic strain and normalized LDH values are proposed as a means of quantifying the influence of thickness differential for a variety of different ratios. Increasing thickness differential was found to decrease the normalized equivalent plastic strain and normalized LDH values, this providing an evaluation of blank formability.
Investigation of the Cause-Effect Relationships between the Exothermic Reaction and the Microstructures of Reactive Ni-Al Particles Produced by High Energy Planetary Ball Milling
Authors: Bernauer Christian , Grohmann Sandra , Angermann Philipp , Dickes Daniel , Holzberger Florian , Amend Pierre , Zaeh Michael F.
Published: May 2021

Reactive particles consisting of nickel and aluminum represent an adaptable heat source for joining applications, since each individual particle is capable of undergoing a self-sustaining exothermic reaction. Of particular interest are particles with intrinsic lamellar microstructures, as they provide large contact areas between the reactants nickel and aluminum. In this work, the exothermic reaction as well as the microstructure of such lamellar reactive particles produced by high energy planetary ball milling were investigated. Based on statistically designed experiments regarding the milling parameters, the heat of reaction was examined by means of differential scanning calorimetry (DSC). A statistical model was derived from the results to predict the heat of reaction as a function of the milling parameters used. This model can be applied to adjust the heat of reaction of the reactive particles depending on the thermal properties of the joining partners. The fabricated microstructures were evaluated by means of scanning electron microscopy (SEM). Through the development of a dedicated SEM image evaluation algorithm, a computational quantification of the contact area between nickel and aluminum was enabled for the first time. A weak correlation between the contact area and the heat of reaction could be demonstrated. It is assumed that the quantification of the contact areas can be further improved by a higher number of SEM images per sample. The findings obtained provide an essential contribution to enable reactive particles as a tailored heat source for joining applications.
Effect of Line Energy Conditions on Mechanical and Fatigue Properties of Ti6Al4V Fabricated by Electron Beam Additive Manufacturing
Authors: Choi Youngsin , Kim Hwi-Jun , Kim Gun-Hee , Lee Chang-Woo , Lee Dong-Geun
Published: May 2021

Additive manufacturing has many process variables and requires additive process optimization. Line energy and scan speed are the main process variables. The objective of this work aims to investigate the effect of changes in line energy and scan speed among the process variables on the mechanical and fatigue properties of the Ti6Al4V specimens fabricated by electron beam additive manufacturing method. The size of the pore inside the specimen was 40~60 μm with the exception of the condition of 0.2 kJ/m, and the specimen with poor fusion of more than 100 μm and gas pore was found to have lower room temperature tensile and fatigue properties compared to the optimal process conditions. As line energy increased, strain hardening occurred, and yield strength and tensile strength increased. The EL:0.3 kJ/m and 800 mm/s condition is a process condition that shows no defects such as unmelted powder and poor fusion, and it represents the best fatigue strength of 400 MPa. The fatigue strength of the specimen performed with hot isostatic pressing after additive manufacturing was measured at 550 MPa, an increase of 150 MPa, which resulted in high fatigue strength enhancement. The crack initiation site and propagation behavior were analyzed by observing the fatigue fracture section of the specimen according to the line energy.
Pleiotropic Actions of Aldehyde Reductase (AKR1A)
Authors: Fujii Junichi , Homma Takujiro , Miyata Satoshi , Takahashi Motoko
Published: May 2021

We provide an overview of the physiological roles of aldehyde reductase (AKR1A) and also discuss the functions of aldose reductase (AKR1B) and other family members when necessary. Many types of aldehyde compounds are cytotoxic and some are even carcinogenic. Such toxic aldehydes are detoxified via the action of AKR in an NADPH-dependent manner and the resulting products may exert anti-diabetic and anti-tumorigenic activity. AKR1A is capable of reducing 3-deoxyglucosone and methylglyoxal, which are reactive intermediates that are involved in glycation, a non-enzymatic glycosylation reaction. Accordingly, AKR1A is thought to suppress the formation of advanced glycation end products (AGEs) and prevent diabetic complications. AKR1A and, in part, AKR1B are responsible for the conversion of d-glucuronate to l-gulonate which constitutes a process for ascorbate (vitamin C) synthesis in competent animals. AKR1A is also involved in the reduction of S-nitrosylated glutathione and coenzyme A and thereby suppresses the protein S-nitrosylation that occurs under conditions in which the production of nitric oxide is stimulated. As the physiological functions of AKR1A are currently not completely understood, the genetic modification of Akr1a could reveal the latent functions of AKR1A and differentiate it from other family members.
Restoration Efficacy of Picea likiangensis var. rubescens Rehder & E. H. Wilson Plantations on the Soil Microbial Community Structure and Function in a Subalpine Area
Authors: Cao Jixin , Shi Songlin , Pan Hong , Chen Zhan , Shang He
Published: May 2021

The knowledge concerning the relationship between vegetation restoration and soil microorganisms is limited, especially at high altitudes. In order to evaluate the restoration efficacy of the reforestation on the soil microbial community, we have examined vegetation composition, edaphic properties and structure and function of different soil microbial groups in two different aged (25- and 40-year-old) Picea likiangensis var. rubescens Rehder & E. H. Wilson (P. rubescens) plantations and the primeval coniferous forest (PCF) dominated by Abies squamata Masters by plot-level inventories and sampling in western Sichuan Province, China. Our results suggested that only the fungal samples in 25-year-old P. rubescens plantation could be distinguished from those in the PCF in both structure and function. The structure and function of the fungal community recovered relatively slowly compared with bacterial and archaeal communities. In addition to the soil chemical properties and tree species composition, the shrub composition was also a key factor influencing the soil microbial community. The P. rubescens plantations were conducive to restoring the soil microbial community in both structure and function. However, there were uncertainties in the variations of the bacterial and archaeal communities with increasing the P. rubescens plantation age.
Clinical Implications of Serum Hepatitis B Virus Pregenomic RNA Kinetics in Chronic Hepatitis B Patients Receiving Antiviral Treatment and Those Achieving HBsAg Loss
Authors: Wu I-Chin , Liu Wen-Chun , Chiu Yen-Cheng , Chiu Hung-Chih , Cheng Pin-Nan , Chang Ting-Tsung
Published: May 2021

Serum hepatitis B virus (HBV) pregenomic RNA (pgRNA) is correlated with covalently closed circular DNA. We aimed to investigate the utility of serum HBV pgRNA in chronic hepatitis B patients receiving nucleos(t)ide analogue treatment and those achieving HBsAg loss. One hundred and eighty-five patients were enrolled for studying long-term HBV pgRNA kinetics during treatment. Twenty patients achieving HBsAg loss after treatment were enrolled for examining HBV pgRNA kinetics around HBsAg loss. HBV pgRNA significantly decreased in the high baseline HBV pgRNA (≥6 log copies/mL) group but significantly increased in the low baseline HBV pgRNA (<4 log copies/mL) group after 3-month entecavir treatment. Among the 20 patients achieving HBsAg loss, 13 (65%) patients had serum HBV pgRNA higher than the limit of detection (LOD, 1466 copies/mL) when they achieved HBsAg loss. Finally, all 20 patients had HBV pgRNA going below the LOD within 3 years after achieving HBsAg loss. In conclusion, baseline serum HBV pgRNA alone is insufficient for predicting the trajectory of HBV pgRNA. Most patients still had HBV pgRNA higher than the LOD when they achieved HBsAg loss. Further studies on HBV pgRNA kinetics around HBsAg loss would provide an enhanced basis for further applications of HBV pgRNA.
Molecular Characterization of Antimicrobial Resistance and Virulence Genes of Bacterial Pathogens from Bovine and Caprine Mastitis in Northern Lebanon
Authors: Abboud Zahie , Galuppo Lucia , Tolone Marco , Vitale Maria , Puleio Roberto , Osman Marwan , Loria Guido Ruggero , Hamze Monzer
Published: May 2021

Mastitis is an infectious disease encountered in dairy animals worldwide that is currently a growing concern in Lebanon. This study aimed at investigating the etiology of the main mastitis-causing pathogens in Northern Lebanon, determining their antimicrobial susceptibility profiles, and identifying their antimicrobial resistance (AMR) genes. A total of 101 quarter milk samples were collected from 77 cows and 11 goats presenting symptoms of mastitis on 45 dairy farms. Bacterial identification was carried out through matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Antimicrobial susceptibility was tested by disc diffusion and broth microdilution methods. Molecular characterization included polymerase chain reaction (PCR) screening for genes encoding extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated AmpC among Enterobacterales isolates, and virulence factors among Staphylococcus isolates. Escherichia coli isolates were subjected to phylogenetic typing by a quadruplex PCR method. The most frequently identified species were Streptococcus uberis (19.2%), Streptococcus agalactiae (15.1%), E. coli (12.3%), and Staphylococcus aureus (10.96%). Gram-positive bacteria were resistant to macrolides and tetracycline, whereas gram-negative bacteria displayed resistance to ampicillin and tetracycline. Two ESBL genes, blaTEM (83.3%) and blaOXA (16.7%), and one AmpC beta-lactamase gene, blaCMY-II (16.7%), were detected among six E. coli isolates, which mainly belonged to phylogenetic group B1. Among Staphylococcus spp., the mecA gene was present in three isolates. Furthermore, four isolates contained at least one toxin gene, and all S. aureus isolates carried the ica operon. These findings revealed the alarming risk of AMR in the Lebanese dairy chain and the importance of monitoring antimicrobial usage.
Virus-Associated Biomarkers in Oropharyngeal and Nasopharyngeal Cancers and Recurrent Respiratory Papillomatosis
Authors: Murono Shigeyuki
Published: May 2021

Nasopharyngeal cancer (NPC) is known to be associated with Epstein–Barr virus (EBV). Pre-treatment and post-treatment detection of plasma cell-free EBV DNA has been shown to be useful as a diagnostic as well as a prognostic factor in NPC. On the other hand, the incidence of human papillomavirus (HPV)-associated oropharyngeal cancer (OPC) is increasing. In contrast to cervical cancer, which is classically known to be an HPV-associated malignancy, HPV testing is not clinically applied for OPC, except for p16 immunostaining as a surrogate marker of HPV infection. One of the major characteristics of HPV-associated OPC is its association with a good prognosis compared with non-HPV-associated OPC. However, some patients still have a poor prognosis. Another characteristic of HPV-associated OPC is the distinct risk factor of high sexual activity. Establishing a biomarker for the prediction of the prognosis before and/or after initial treatment, as well as for diagnosis in populations at high risk, is of marked interest. With this background, HPV DNA detection in plasma and oral rinses has become an area of focus. In this review, the current significance of HPV DNA detection in plasma and oral rinse samples, as well as serum HPV antibody levels, is evaluated.
Omega-3 Fatty Acids and Their Role in Pediatric Cancer
Authors: Podpeskar Alexandra , Crazzolara Roman , Kropshofer Gabriele , Hetzer Benjamin , Meister Bernhard , Müller Thomas , Salvador Christina
Published: May 2021

Background: Malnutrition is common in children with cancer and is associated with adverse clinical outcomes. The need for supportive care is becoming ever more evident and the role of nutrition in oncology is still not sufficiently understood. In particular, the consequences of macro- and micronutrient deficiencies require further research. As epidemiological data suggest anti-tumoral properties of omega-3 (n-3) polyunsaturated fatty acids (PUFAs), we reviewed the role of nutrition and n-3 supplementation in pediatric oncology. Methods: A comprehensive literature search was conducted on PubMed through 5 February 2021 to select meta-analyses, systematic reviews, observational studies, and individual randomized controlled trials (RCTs) on macro- and micronutrient supplementation in pediatric oncology. The search strategy included the following medical subject headings (MeSH) and keywords: “childhood cancer”, “pediatric oncology”, “nutritional status”, “malnutrition”, and “omega-3-fatty-acids”. The reference lists of all relevant articles were screened to include potentially pertinent studies. Results: We summarize evidence about the importance of adequate nutrition in childhood cancer and the role of n-3 PUFAs and critically interpret findings. Possible effects of supplementation on the nutritional status and benefits during chemotherapy are discussed as well as strategies for primary and secondary prevention. Conclusion: We here describe the obvious benefits of omega-3 supplementation in childhood cancer. Further large scale clinical trials are required to verify potential anti-cancer effects of n-3 fatty acids.
Reduction of Cardio-Metabolic Risk and Body Weight through a Multiphasic Very-Low Calorie Ketogenic Diet Program in Women with Overweight/Obesity: A Study in a Real-World Setting
Authors: Tragni Elena , Vigna Luisella , Ruscica Massimiliano , Macchi Chiara , Casula Manuela , Santelia Alfonso , Catapano Alberico L. , Magni Paolo
Published: May 2021

Background: The prevention and treatment of obesity and its cardio-metabolic complications are relevant issues worldwide. Among lifestyle approaches, very low-calorie ketogenic diets (VLCKD) have been shown to lead to rapid initial weight loss, resulting in better long-term weight loss maintenance. As no information on VLCKD studies carried on in a real-world setting are available, we conducted this multi-centre study in a real-world setting, aiming at assessing the efficacy and the safety of a specific multiphasic VLCKD program in women with overweight or obesity. Methods: A multi-center, prospective, uncontrolled trial was conducted in 33 outpatient women (age range 27–60 y) with overweight or obesity (BMI: 30.9 ± 2.7 kg/m2; waist circumference: 96.0 ± 9.4 cm) who started a VLCKD dietary program (duration: 24 weeks), divided into four phases. The efficacy of VLCKD was assessed by evaluating anthropometric measures and cardiometabolic markers; liver and kidney function biomarkers were assessed as safety parameters. Results: The VLCKD program resulted in a significant decrease of body weight and BMI (−14.6%) and waist circumference (−12.4%). At the end of the protocol, 33.3% of the participants reached a normal weight and the subjects in the obesity range were reduced from 70% to 16.7%. HOMA-IR was markedly reduced from 3.17 ± 2.67 to 1.73 ± 1.23 already after phase 2 and was unchanged thereafter. Systolic blood pressure decreased after phase 1 (−3.5 mmHg) and remained unchanged until the end of the program. Total and LDL cholesterol and triglycerides were significantly reduced by VLCKD along with a significant HDL cholesterol increase. Liver, kidney and thyroid function markers did not change and remained within the reference range. Conclusions: The findings of a multi-center VLCKD program conducted in a real-world setting in a cohort of overweight/obese women indicate that it is safe and effective, as it results in a major improvement of cardiometabolic parameters, thus leading to benefits that span well beyond the mere body weight/adiposity reduction.
A Case-Series Observation of Sweat Rate Variability in Endurance-Trained Athletes
Authors: Smith JohnEric W. , Bello Marissa L. , Price Ffion G.
Published: May 2021

Adequate fluid replacement during exercise is an important consideration for athletes, however sweat rate (SR) can vary day-to-day. The purpose of this study was to investigate day-to-day variations in SR while performing self-selected exercise sessions to evaluate error in SR estimations in similar temperature conditions. Thirteen endurance-trained athletes completed training sessions in a case-series design 1x/week for a minimum 30 min of running/biking over 24 weeks. Body mass was recorded pre/post-training and corrected for fluid consumption. Data were split into three Wet-Bulb Globe Thermometer (WBGT) conditions: LOW (<10 °C), MOD (10–19.9 °C), HIGH (>20 °C). No significant differences existed in exercise duration, distance, pace, or WBGT for any group (p > 0.07). Significant differences in SR variability occurred for all groups, with average differences of: LOW = 0.15 L/h; MOD = 0.14 L/h; HIGH = 0.16 L/h (p < 0.05). There were no significant differences in mean SR between LOW-MOD (p > 0.9), but significant differences between LOW-HIGH and MOD-HIGH (p < 0.03). The assessment of SR can provide useful data for determining hydration strategies. The significant differences in SR within each temperature range indicates a single assessment may not accurately represent an individual’s typical SR even in similar environmental conditions.
Co-Occurrence and Clustering of Sedentary Behaviors, Diet, Sugar-Sweetened Beverages, and Alcohol Intake among Adolescents and Adults: The Latin American Nutrition and Health Study (ELANS)
Authors: B. Leme Ana Carolina , Ferrari Gerson , Fisberg Regina M. , Kovalskys Irina , Gómez Georgina , Cortes Lilia Yadira , Yépez Gárcia Martha Cecilia , Herrera-Cuenca Marianella , Rigotti Attilo , Liria-Domínguez María Reyna , Fisberg Mauro
Published: May 2021

Poor diet, sedentary behaviors, sugar-sweetened beverages (SSB) and alcohol intake seem to co-exist in complex ways that are not well understood. The aim of this study was to provide an understanding of the extent to which unhealthy behaviors cluster in eight Latin America countries. A secondary aim was to identify socio-demographic characteristics associated with these behaviors by country. Data from adolescents and adults from the “Latin American Health and Nutrition Study” was used and the prevalence of screen-time, occupational and transportation–sedentary time, socializing with friends, poor diet, SSB and alcohol intake, alone and in combination, were identified. The eight Latin America (LA) countries added to analyses were: Argentina, Brazil, Chile, Colombia, Costa Rica, Ecuador, Peru, and Venezuela. Logistic regression was used to estimate associations between ≥2 behaviors clustering, socio-demographics and weight status. Among 9218 individuals, the most prevalent behaviors were transportation and occupation–sedentary time, SSB and alcohol intake. Younger, female, married/living with a partner, low and middle-income and obese individuals had higher chances for these clustering behaviors. These results provide a multi-country level of understanding of the extent to which behaviors co-occur in the LA population.
Strong and Bitter Vegetables from Traditional Cultivars and Cropping Methods Improve the Health Status of Type 2 Diabetics: A Randomized Control Trial
Authors: Thorup Anne Cathrine , Kristensen Hanne Lakkenborg , Kidmose Ulla , Lambert Max Norman Tandrup , Christensen Lars Porskjær , Fretté Xavier , Clausen Morten Rahr , Hansen Steen Møller , Jeppesen Per Bendix
Published: May 2021

Vegetables rich in bitter-tasting phytochemicals may exert enhanced beneficial effects against key factors associated with type two diabetes (T2D). This study investigates whether selected cultivars of bitter and strong-tasting (BST) Brassica and root vegetables exert greater health benefits on T2D patients compared to equivalent modern mild and sweet tasting (MST) vegetables. A 12-week randomized, controlled, parallel intervention study involved 92 T2D patients, who were allocated three different diets: (1) 500 g daily of bitter and strong-tasting (BST) vegetables; (2) 500 g daily of mild and sweet-tasting (MST) vegetables; (3) 120 g daily MST normal diet (control). Both vegetable diets contained root vegetables and cabbages selected based on sensory differences and content of phytochemicals. Prior to and after the study, all participants underwent an oral glucose tolerance test (OGTT), 24 h blood pressure measurements, DEXA scans, and fasted blood samples. Both diets high in vegetables significantly reduced the participants’ BMI, total body fat mass, and HbA1c levels compared to control, but in the BST group, significant differences were also found regarding incremental area under the curve glucose 240 min (OGTT) and fasting glucose levels. A high daily intake of root vegetables and cabbages showed significant health improvements in both vegetable groups. BST vegetables had the greatest impact on insulin sensitivity, body fat mass, and blood pressure compared to control; moreover, they further improved glycemic control compared to MST vegetables.
Lifestyle and Treatment Adherence Intervention after a Coronary Event Based on an Interactive Web Application (EVITE): Randomized Controlled Clinical Trial Protocol
Authors: Bernal-Jiménez María Ángeles , Calle-Pérez Germán , Gutiérrez-Barrios Alejandro , Gheorghe Livia , Solano-Mulero Ana María , Rodríguez-Martín Amelia , Tur Josep A. , Vázquez-García Rafael , Santi-Cano María José
Published: May 2021

Coronary heart disease is one of the main causes of morbimortality around the world. Patients that survive a coronary event suffer a high risk of readmission, relapse and mortality, attributed to the sub-optimal control of cardiovascular risk factors (CVRF), which highlights the need to improve secondary prevention strategies aimed at improving their lifestyle and adherence to treatment. Through a randomized controlled clinical trial, this study aims to evaluate the effect of an intervention involving an online health application supported by a mobile telephone or tablet (mHealth) on lifestyle (diet, physical activity, and tobacco consumption) and treatment adherence among people with coronary heart disease after percutaneous coronary intervention. The sample will comprise 240 subjects (120 in each arm: intervention and usual care). They are assessed immediately and nine months after their hospital discharge about sociodemographic, clinical, CVRF, lifestyle, and treatment adherence characteristics. The educative intervention, involving a follow-up and self-monitoring, will be performed using an online mHealth tool consisting of an application for mobile phones and tablets. The quantitative primary outcomes from the two groups will be compared using an analysis of covariance (ANCOVA) adjusted for age and gender. A multivariate analysis will be performed to examine the association of the intervention with lifestyle habits, the control of CVRFs, and outcomes after discharge in terms of the use of health services, emergency visits, cardiovascular events and readmissions.
Performance of the LIAISON® SARS-CoV-2 Antigen Assay vs. SARS-CoV-2-RT-PCR
Authors: Fiedler Melanie , Holtkamp Caroline , Dittmer Ulf , Anastasiou Olympia E.
Published: May 2021

We aimed to evaluate the LIAISON® SARS-CoV-2 antigen assay (DiaSorin), comparing its performance to real-time polymerase chain reaction (RT-PCR) for the detection of SARS-CoV-2 RNA. 182 (110 PCR-positive and 72 PCR-negative) nasopharyngeal swab samples were taken for the detection of SARS-CoV-2. RT-PCR and antigen assay were performed using the same material. The sensitivity and specificity of the antigen assay were calculated for different cut-offs, with RT-PCR serving as the reference method. Stored clinical samples that were positive for other respiratory viruses were tested to evaluate cross-reactivity. One third (33/110, 30%) were falsely classified as negative, while no false positives were found using the 200 TCID50/mL cut-off for the SARS-CoV-2 antigen as proposed by the manufacturer. This corresponded to a sensitivity of 70% (60–78%) and a specificity of 100% (94–100%). Lowering the cut-off for positivity of the antigen assay to 22.79 or 57.68 TCID50/mL increased the sensitivity of the method, reaching a sensitivity of 92% (85–96%) vs. 79% (70–86%) and a specificity of 81% (69–89%) vs. 99% (91–100%), respectively. The antigen assay reliably detected samples with high SARS-CoV-2 viral loads (≥106 copies SARS-CoV-2/mL), while it cannot differentiate between negative and low positive samples. Cross-reactivity toward other respiratory viruses was not detected.
Fire Ant Venom Alkaloids: Possible Control Measure for Soilborne and Foliar Plant Pathogens
Authors: Dawadi Sujan , Baysal-Gurel Fulya , Addesso Karla M. , Liyanapathiranage Prabha , Simmons Terri
Published: May 2021

The purpose of this study was to evaluate fire ant venom alkaloids and an alarm pheromone analog against several plant pathogens, including Botrytis cinerea, Fusarium oxysporum, Phytophthora nicotianae, P. cryptogea, Pseudomonas syringae, Phytopythium citrinum, Rhizoctonia solani, Sclerotonia rolfsii, Xanthomonas axonopodis, and X. campestris. All pathogens were tested against red imported fire ant venom alkaloid extract and alarm pheromone compound for growth inhibition in in vitro assay. The venom alkaloid extract inhibited fungal and oomycete pathogens. Neither of the treatments were effective against bacterial pathogens. Three soilborne pathogens, P. nicotianae, R. solani, F. oxysporum, and one foliar pathogen, B. cinerea were selected for further in-vivo assays on impatiens (Impatiens walleriana ‘Super Elfin XP violet’). Total plant and root weight were higher in venom alkaloid treated plants compared to an inoculated control. The venom alkaloid treatment reduced damping-off, root rot severity, and pathogen recovery in soilborne pathogen inoculated plants. Similarly, venom alkaloid reduced Botrytis blight. However, higher venom rates caused foliar phytotoxicity on plants. Therefore, additional work is needed to evaluate rates of venom alkaloids or formulations to eliminate negative impacts on plants. Overall, these results suggest that red imported fire ant venom alkaloids may provide a basis for new products to control soilborne and foliar plant pathogens.
Microfluidic and Lab-on-a-Chip Systems for Cutaneous Wound Healing Studies
Authors: Shabestani Monfared Ghazal , Ertl Peter , Rothbauer Mario
Published: May 2021

Cutaneous wound healing is a complex, multi-stage process involving direct and indirect cell communication events with the aim of efficiently restoring the barrier function of the skin. One key aspect in cutaneous wound healing is associated with cell movement and migration into the physically, chemically, and biologically injured area, resulting in wound closure. Understanding the conditions under which cell migration is impaired and elucidating the cellular and molecular mechanisms that improve healing dynamics are therefore crucial in devising novel therapeutic strategies to elevate patient suffering, reduce scaring, and eliminate chronic wounds. Following the global trend towards the automation, miniaturization, and integration of cell-based assays into microphysiological systems, conventional wound healing assays such as the scratch assay and cell exclusion assay have recently been translated and improved using microfluidics and lab-on-a-chip technologies. These miniaturized cell analysis systems allow for precise spatial and temporal control over a range of dynamic microenvironmental factors including shear stress, biochemical and oxygen gradients to create more reliable in vitro models that resemble the in vivo microenvironment of a wound more closely on a molecular, cellular, and tissue level. The current review provides (a) an overview on the main molecular and cellular processes that take place during wound healing, (b) a brief introduction into conventional in vitro wound healing assays, and (c) a perspective on future cutaneous and vascular wound healing research using microfluidic technology.
The Impact of Heat Stress on Morpho-Physiological Response and Expression of Specific Genes in the Heat Stress-Responsive Transcriptional Regulatory Network in Brassica oleracea
Authors: Moradpour Mahdi , Abdullah Siti Nor Akmar , Namasivayam Parameswari
Published: May 2021

Knowledge of heat-tolerant/sensitive cultivars based on morpho-physiological indicators and an understanding of the action and interaction of different genes in the molecular network are critical for genetic improvement. To screen these indicators, the physiological performance of two different varieties of white and red cabbages (B. oleracea var. capitate f. alba and f. rubra, respectively) under heat stress (HS) and non-stress (NS) was evaluated. Cultivars that showed considerable cell membrane thermostability and less reduction in chlorophyll content with better head formation were categorized as the heat-tolerant cultivars (HTC), while those with reduction in stomatal conductance, higher reduction incurred in chlorophyll and damage to thylakoid membranes are categorized as the heat-sensitive cultivars (HSC). Expression profiling of key genes in the HS response network, including BoHSP70 (HEAT SHOCK PROTEIN 70), BoSCL13 (SCARECROW-LIKE 13) and BoDPB3-1 (transcriptional regulator DNA POLYMERASE II SUBUNIT B3-1 (DPB3-1))/NUCLEAR FACTOR Y SUBUNIT C10 (NF-YC10), were evaluated in all cultivars under HS compared to NS plants, which showed their potential as molecular indicators to differentiate HTC from HSC. Based on the results, the morphophysiological and molecular indicators are applicable to cabbage cultivars for differentiating HTC from HSC, and potential target genes for genome editing were identified for enhancing food security in the warmer regions of the world.
Valorization of Citrus Co-Products: Recovery of Bioactive Compounds and Application in Meat and Meat Products
Authors: Nieto Gema , Fernández-López Juana , Pérez-Álvarez José A. , Peñalver Rocío , Ros-Berruezo Gaspar , Viuda-Martos Manuel
Published: May 2021

Citrus fruits (orange, lemon, mandarin, and grapefruit) are one of the most extensively cultivated crops. Actually, fresh consumption far exceeds the demand and, subsequently, a great volume of the production is destined for the citrus-processing industries, which produce a huge quantity of co-products. These co-products, without proper treatment and disposal, might cause severe environmental problems. The co-products obtained from the citrus industry may be considered a very important source of high-added-value bioactive compounds that could be used in the pharmaceutical, cosmetic, and dietetic industries, and mainly in the food industry. Due to consumer demands, the food industry is exploring a new and economical source of bioactive compounds to develop novel foods with healthy properties. Thus, the aim of this review is to describe the possible benefits of citrus co-products as a source of bioactive compounds and their applications in the development of healthier meat and meat products.
Understanding Potential Heavy Metal Contamination, Absorption, Translocation and Accumulation in Rice and Human Health Risks
Authors: Zakaria Zuliana , Zulkafflee Nur Syahirah , Mohd Redzuan Nurul Adillah , Selamat Jinap , Ismail Mohd Razi , Praveena Sarva Mangala , Tóth Gergely , Abdull Razis Ahmad Faizal
Published: May 2021

Rice is a worldwide staple food and heavy metal contamination is often reported in rice production. Heavy metal can originate from natural sources or be present through anthropogenic contamination. Therefore, this review summarizes the current status of heavy metal contamination in paddy soil and plants, highlighting the mechanism of uptake, bioaccumulation, and health risk assessment. A scoping search employing Google Scholar, Science Direct, Research Gate, Scopus, and Wiley Online was carried out to build up the review using the following keywords: heavy metals, absorption, translocation, accumulation, uptake, biotransformation, rice, and human risk with no restrictions being placed on the year of study. Cadmium (Cd), arsenic (As), and lead (Pb) have been identified as the most prevalent metals in rice cultivation. Mining and irrigation activities are primary sources, but chemical fertilizer and pesticide usage also contribute to heavy metal contamination of paddy soil worldwide. Further to their adverse effect on the paddy ecosystem by reducing the soil fertility and grain yield, heavy metal contamination represents a risk to human health. An in-depth discussion is further offered on health risk assessments by quantitative measurement to identify potential risk towards heavy metal exposure via rice consumption, which consisted of in vitro digestion models through a vital ingestion portion of rice.
Volatile Compounds, Sensory Profile and Phenolic Compounds in Fermented Rice Bran
Authors: Ardiansyah  , Nada Annisa , Rahmawati Nuraini Tiara Indah , Oktriani Annisa , David Wahyudi , Astuti Rizki Maryam , Handoko Dody Dwi , Kusbiantoro Bram , Budijanto Slamet , Shirakawa Hitoshi
Published: May 2021

Rice bran (RB), a by-product of the rice milling process, is a rich source of bioactive compounds. Current studies have suggested that fermentation can enhance the bioactivities of RB. This study is aimed to analyse the volatile compounds and sensory profile of fermented RB from two cultivars (Inpari 30 and Cempo Ireng) that are well-known in Indonesia, as well as to measure total phenolic content (TPC) and antioxidant activity. Volatile compounds of fermented RB were analyzed using gas chromatography-mass spectrometry combined with headspace-solid phase microextraction. The optimum TPC and antioxidant activity were observed after 72 h fermentation of RB. The 55 volatile compounds were identified in fermented and non-fermented RB. They were classified into alcohols, aldehydes, acids, ketones, phenols, esters, benzene, terpenes, furans, lactone, pyridines, pyrazines, and thiazoles. Volatile compounds were significantly different among the varieties. The sensory analysis showed that the panelists could differentiate sensory profiles (color, taste, flavor, and texture) between the samples. Fermentation can enhance the acceptance of RB. These studies may provide opportunities to promote the production of fermented RB as a functional ingredient with enhanced bioactivity for health promotion.
Study on Pressure Change Rate of the Automatic Pressure Regulating Valve in the Electronic-Controlled Pneumatic Braking System of Commercial Vehicle
Authors: Bao Hanwei , Wang Zaiyu , Liu Zihao , Li Gangyan
Published: May 2021

In contrast to the traditional pneumatic braking system, the electronic-controlled pneumatic braking system of commercial vehicles is a new system and can remedy the defects of the conventional braking system, such as long response time and low control accuracy. Additionally, it can adapt to the needs and development of autonomous driving. As the key pressure regulating component in electronic-controlled pneumatic braking system of commercial vehicles, automatic pressure regulating valves can quickly and accurately control the braking pressure in real time through an electronic control method. By aiming at improving driving comfort on the premise of ensuring braking security, this paper took the automatic pressure regulating valve as the research object and studied the pressure change rate during the braking process. First, the characteristics of the automatic pressure regulating valve and the concept of the pressure change rate were elaborated. Then, with the volume change of automatic pressure regulating valve in consideration, the mathematical model based on gas dynamics and the association model between pressure change rate and vehicle dynamic model was established in MATLAB/Simulink and analyzed. Next, through the experimental test of a sample product, the mathematical models have been verified. Finally, the key structure parameters affecting the pressure change rate of the automatic pressure regulating valve and the influence law have been identified; therefore, appropriate design advice and theoretical support have been provided to improve driving comfort.
Health and Housing Energy Expenditures: A Two-Part Model Approach
Authors: Lima Fátima , Ferreira Paula , Leal Vítor
Published: May 2021

Interest in the interaction between energy and health within the built environment has been increasing in recent years, in the context of sustainable development. However, in order to promote health and wellbeing across all ages it is necessary to have a better understanding of the association between health and energy at household level. This study contributes to this debate by addressing the case of Portugal using data from the Household Budget Survey (HBS) microdata database. A two-part model is applied to estimate health expenditures based on energy-related expenditures, as well as socioeconomic variables. Additional statistical methods are used to enhance the perception of relevant predictors for health expenditures. Our findings suggest that given the high significance and coefficient value, energy expenditure is a relevant explanatory variable for health expenditures. This result is further validated by a dominance analysis ranking. Moreover, the results show that health gains and medical cost reductions can be a key factor to consider on the assessment of the economic viability of energy efficiency projects in buildings. This is particularly relevant for the older and low-income segments of the population.