bluuebunny/crossref_metadata_2025 huggingface dataset:
The space-time structure of sound produced by stacked rotors in hover using Vold-Kalman filters and proper orthogonal decomposition
Authors: Tinney Charles E , Zhao-Dubuc Yingjun , Valdez John
Published: September 2023

Proper orthogonal decomposition and the Vold-Kalman order tracking filter are combined to evaluate the most energetic components of the sound field produced by a coaxial, co-rotating rotor in hover. The study leverages the database generated by Tinney and Valdez (2020; AIAA J. vol. 58, no. 4) comprising an array of eight stationary microphones oriented to capture the acoustic near-field below the rotor disk plane where ground observers are expected to reside and where concerns over community annoyance are greatest. Changes to rotor conditions focus primarily on rotor speed and index angle (angular separation between the upper and lower rotors), as this has the greatest effect on the sound field produced by stacked rotors by way of the constructive and destructive interference of the sound generated, separately, by the upper and lower rotors. Proper orthogonal decomposition is performed using a kernel constructed from auto and cross-spectral densities of the spatially coherent sound field. The eigenvectors demonstrate the spatial extent of the sound field for discrete frequencies corresponding to the first few blade pass frequency harmonics. In order to improve the clarity and accuracy of the low-order reconstructions, a second generation, Vold-Kalman multi-order tracking filter is employed to isolate discrete frequencies. Relative to conventional spectral filtering methods, the Vold-Kalman filter is performed in the time-domain and is shown to accurately isolate discrete tones while preserving changes to the phase of the signal. Findings from this analysis reveal the effect of index angle on the spatial make-up of the coherent sound field produced by hovering stacked rotors.
The Role of Mechanical Thrombectomy for Acute Massive Pulmonary Embolism in a Patient With Unilateral Lung Transplant and Atrial Septal Defect
Authors: Altun Izzet , Zhao Yuanlong , Basnet Suresh , Raymond Aislynn , Fang Adam , Nezami Nariman
Published: September 2023

Purpose:

The risk of thromboembolic disease is high in patients with lung transplantation and is associated with significant morbidity and mortality with single healthy transplanted lung. We present a case involving successful endovascular management of life-threatening acute massive pulmonary embolism (PE) in a patient with single lung transplant and atrial septal defect (ASD).

Case Report:

A 65-year-old man with a history of interstitial lung disease status post single left orthotopic lung transplant in 2012 presented with acute massive PE and clot burden in the pulmonary arteries of the transplanted left lung. Severe right heart dysfunction, hemodynamic instability, and requirement for vasopressors persisted post systemic thrombolytic therapy. As a result, the patient underwent successful endovascular mechanical thrombectomy with immediate improvement in oxygen saturation and hemodynamic status. The procedure was performed without adverse outcomes or paradoxical embolization despite the presence of ASD. The right heart dysfunction resolved, the patient was extubated the next day, and was discharged to home 2 days post procedure.

Conclusions:

Endovascular mechanical thrombectomy was safely used to treat acute massive PE in a single transplanted lung in the presence of ASD.

Clinical Impact

Endovascular mechanical thrombectomy could be safely utilized to treat patients with lung transplant and acute massive or submassive pulmonary embolism. However, safely of mechanical thrombectomy should be determined in case-based scenarios and based on time interval from transplantation to when the thrombectomy is required.
Encouraging, Increasing, and Inviting Fathers From Different Cultural and Religious Practices in Early Childhood Education
Authors: Gardner Sinsery , Alremaih Latifah A. , Walton Marlene
Published: September 2023

Salim is the father of a young girl who attends Small Fortune Preschool located in the suburb of Detroit. Although he would like to regularly participate in his child’s school events, his beliefs restrain him from helping in the classrooms headed by female teachers, unless his wife is present. This case demonstrates some of the challenges that fathers from different cultural and religious backgrounds may experience in being an involved father in a preschool setting. The purpose of this case is to inform teachers about cultural barriers to family, specifically father involvement from minoritized culture and religious practices.
Research Progress of STEM Education Based on Visual Bibliometric Analysis
Authors: Cai Zhiling , Zhu Jinxing , Tian Saiqi
Published: September 2023

Nowadays, science, technology, engineering, mathematics (STEM) education has been developed and implemented in many countries. To better understand the research progress in STEM education worldwide, we analyzed 1910 publications collected from the Web of Science Core Collection database. This bibliometric study was performed through software VOSviewer and CiteSpace. Results showed that the research on STEM education will remain thriving in the following years. The USA is the most active country in the research field of STEM education, and the whole world cooperated closely. The majorities of significant institutions in this field belong to the USA. International Journal of STEM Education, Journal of Science Education and Technology and Science Education are the main publications. According to the cluster results of keywords, STEM, STEM education, self-efficacy, technology constitute the knowledge base for STEM education studies. Based the analysis results of keywords and burst detection, STEM achievements, different approaches to discipline integration, educational equality, and teacher education will be the research hotspot in the future. The results give a comprehensive overview on STEM education for scholars quickly understanding the overall progress.
Goodbye Florida, I’m Out! For Good
Authors: Thornhill Ted
Published: September 2023

Florida governor Ron DeSantis, aided by the state’s Republican-controlled legislature and allied functionaries, instigated a racist and anti-Black crusade against the teaching of accurate U.S. racial history as well as diversity, equity, and inclusion initiatives. Their actions have caused considerable harm to students, families, and educators. In this article, I recount my experience teaching at a public university in my home state of Florida during the Trump-DeSantis era, engaging in antiracist public scholarship, and ultimately deciding it was time to “get out.”
Dentists’ Approaches to Treatment Decision-Making for People with Dementia: A Qualitative Study
Authors: Geddis-Regan A. , Abley C. , Exley C. , Wassall R.
Published: September 2023

Introduction:

Globally, the number of people living with dementia (PLwD) is projected to increase substantially. Dentists will encounter an increasing number of PLwD retaining natural teeth who need dental care. Dental treatment planning can be complex for PLwD as both oral diseases and dental treatment can be detrimental to patients’ oral and social function and comfort. In addition, patients may lack the capacity to make decisions about their treatment, posing further challenges for dentists.

Objective:

This study aimed to explore dentists’ approach to treatment decision-making with or for PLwD.

Methods:

Semistructured one-to-one interviews were completed with 22 dentists between April 2020 and March 2021. Data generation and analysis followed the principles of constructivist grounded theory with data being collected and analyzed simultaneously using a maximum variation sample. Interviews were recorded, transcribed verbatim, and then analyzed, leading to a theoretical understanding of how dentists approach treatment decisions for PLwD.

Results:

Four data categories describe dentists’ approaches to treatment decision-making for PLwD. Dentists sought to provide individualized care for PLwD. However, they described planning care based on risk-benefit analyses that primarily considered biomedical factors and generic assumptions. There was an underemphasis on the psychological or social implications of dental care or its delivery. Furthermore, while some dentists attempted to involve patients in treatment decisions, they reported fewer attempts to identify the views and preferences of PLwD identified as lacking decisional capacity. In this scenario, dentists reported leading the decision-making process with little regard for patients’ known or identified preferences and minimal involvement of PLwD’s family.

Conclusions:

Dentists acknowledge the complexity in treatment decision-making for PLwD yet focused heavily on biomedical considerations, with an underemphasis on individuals’ preferences and autonomy. Considering psychosocial aspects of care alongside biomedical factors is essential to support holistic person-centered care for this growing patient cohort.

Knowledge Transfer Statement:

This study highlighted that dentists may only be considering biomedical aspects of care when considering what treatment is appropriate for people living with dementia. When making or supporting treatment decisions, dentists should explore patients’ preferences and actively consider relevant psychosocial factors. These can be actively identified through considered discussions with patients as well as their family members. Gathering this information should support more person-centered and value-concordant decision-making for people living with dementia.
Trends in hemorrhagic stroke incidence and mortality in a National Stroke Registry of a multi-ethnic Asian population
Authors: Lim Mervyn Jun Rui , Zheng Huili , Zhang Zheting , Sia Ching Hui , Tan Benjamin Yong-Qiang , Hock Ong Marcus Eng , Nga Vincent Diong Weng , Yeo Tseng Tsai , Ho Andrew Fu Wah
Published: September 2023

Introduction:

High-quality epidemiological data on hemorrhagic stroke (HS) and its subtypes, intracerebral hemorrhage (ICH) and subarachnoid hemorrhage (SAH), remains limited in Asian ethnicities. We investigated the trends in HS incidence and 30-day mortality from 2005 to 2019 in a multi-ethnic Asian population from a national registry.

Patients and methods:

Data on all stroke cases from the Singapore Stroke Registry from 2005 to 2019 were collected. Cases were defined using centrally adjudicated review of diagnosis codes. Death outcomes were obtained by linkage with the national death registry. Incidence (per 100,000 people) and 30-day mortality (per 100 people) were measured as crude and age-standardized rates. Trends were analyzed using linear regression.

Results:

We analyzed 19,017 cases of HS (83.9% ICH; 16.1% SAH). From 2005 to 2019, age-standardized incidence rates (ASIR) for HS remained stable from 34.4 to 34.5. However, age-standardized mortality rates (ASMR) decreased significantly from 29.5 to 21.4 ( p < 0.001). For ICH, ASIR remained stable while ASMR decreased from 30.4 to 21.3 ( p < 0.001); for SAH, ASIR increased from 2.7 to 6.0 ( p = 0.006) while ASMR remained stable. In subgroup analyses, HS incidence increased significantly in persons <65 years (from 18.1 to 19.6) and Malays (from 39.5 to 49.7).

Discussion:

From 2005 to 2019, ASIR of HS remained stable while ASMR decreased. Decreasing ASMR reflects improvements in the overall management of HS, consistent with global trends.

Conclusion:

Population health efforts to address modifiable risk factors for HS in specific demographic subgroups may be warranted to reduce incidence and mortality of HS.
Exploring online experiences, cyberbullying and wellbeing for young people looked after in Wales: An analysis of the school health research network 2017/18 survey
Authors: Roberts Louisa M. , Wood Sophie , Corliss Cindy , Anthony Rebecca
Published: September 2023

For young people the online world affords creative, entertainment and socialisation opportunities but also poses risks including cyberbullying, grooming and harmful content. Little is known about online experiences of care-experienced young people, a vulnerable group who may benefit from online experiences but may be at increased risk online. This cross-sectional study used data from 11- to 16-year-olds in Wales from the School Health Research Network (SHRN) Student Health and Wellbeing Survey 2017/18 ( n = 103,971). Cyberbullying, face-to-face bullying and other online experiences were analysed. The prevalence of these experiences and associated mean wellbeing scores were compared for young people in care ( n = 1,921) and not in care ( n = 83,551), controlling for socio-demographic variables gender, ethnicity and year group. Higher numbers of young people in care were involved in cyberbullying, bullying, sharing explicit images, problematic social media use and regular contact with online only friends. These online experiences were associated with lower mean wellbeing scores. Lower numbers of young people in care had access to smartphones and social networking sites from age 12, and lower numbers were regularly in online contact with close friends and a wider peer group. These online experiences were associated with higher mean wellbeing scores. Young people in care appear to be less involved with online experiences that positively impact wellbeing and more involved in online experiences that negatively impact wellbeing. Promoting positive online experiences and education around problematic social media use may be particularly valuable in helping young people living in care develop healthy, safe and positive online lives.
How Relational Publics Become Scandal Audiences: Values and the construction of scandal
Authors: Pozner Jo-Ellen , Hannigan Timothy R.
Published: September 2023

We are interested in examining the process of scandal creation through the lens of the audience. Extant work tends to address either the effects of organizational scandal, or the role of the media and social control agents in scandal creation, neglecting the audience. To address this gap, we draw on the sociological concept of the relational public to explore how individual or small group assessments become widely held social evaluations among scandal audiences. We develop a three-stage model of organizational scandal creation: first, scandal entrepreneurs and the media frame an organization’s behavior as transgressive to media consumers who react within the relational publics they constitute; next, members of those relational publics judge the act in light of their values; finally, as relational publics spread their judgment to adjacent groups, they aggregate and assemble into a scandal audience, activating the scandal. Our model adds to media-centered theories of scandal construction by highlighting the role of heterogeneous audiences and their values, building a nuanced understanding of the process of social evaluation in scandal creation.
Effects of allyl isothiocyanate fumigation on medicinal plant root knot disease control, plant survival, and the soil bacterial community
Authors: Li Yingbin , Lu Daqing , Xia Yan , Xu Xinjing , Huang Huichuan , Mei Xinyue , Yang Min , Li Jianqiang , Zhu Shusheng , Liu Yixiang , Zhang Zhiping
Published: September 2023

Background

Allyl isothiocyanate (AITC) is a natural product with high volatility that is used as a biofumigant to alleviate soil-borne plant diseases, and problems such as root knot nematodes (RKNs) that necessitate continuous cropping. However, little research has assessed the effects of AITC fumigation on medicinal plants.

Results

AITC significantly reduced the population of RKNs in soil (p < 0.0001) and showed an excellent RKN disease control effect within 6 months after sowing Panax notoginseng (p < 0.0001). The seedling survival rate of 2-year-old P. notoginseng was approximately 1.7-fold higher after soil treatment with AITC (p = 0.1008). 16S rRNA sequencing indicated that the AITC treatment affected bacterial richness rather than diversity in consecutively cultivated (CC) soil. Furthermore, biomarkers with statistical differences between AITC-treated and untreated CC soil showed that Pirellulales (order), Pirellulaceae (family), Pseudomonadaceae (family), and Pseudomonas (genus) played important roles in the AITC-treated group. In addition, the microbiome functional phenotypes predicted using the BugBase tool suggested that AITC treatment is more conducive to improving CC soil through changes in the bacterial community structure. Crucially, our research also suggested that AITC soil treatment significantly increases soil organic matter (p = 0.0055), total nitrogen (p = 0.0054), and available potassium (p = 0.0373), which promotes the survival of a succeeding medicinal plant (Polygonatum kingianum).

Conclusion

AITC is an ecologically friendly soil treatment that affects the top 10 bacterial richness but not diversity. It could also provide a basis for a useful agricultural soil management measure to alleviate soil sickness.
Comparing exercise determinants between Black and White older adults with heart failure
Authors: Kaushal Navin , Nemati Donya , Mann-Krzisnik Dylan , de la Colina Adrián Noriega
Published: September 2023

Background

Heart Failure is a leading cause of mortality among older adults. Engaging in regular exercise at moderate-to-vigorous intensity has been shown to improve survival rates. Theory-informed methodologies have been recommended to promote exercise, but limited application of theoretical framework has been conducted for understanding racial disparities among older adults with heart failure. This study aimed to use the Health Belief Model to compare exercise behavior determinants between Black and White older adults diagnosed with heart failure.

Methods

The HF-ACTION Trial is a multi-site study designed to promote exercise among individuals with heart failure that randomized participants to an experimental (three months of group exercise sessions followed by home-based training) or control arm. The present study used structural equation modeling to test the change in Health Belief Model constructs and exercise behavior across 12 months among older adults.

Results

Participants (n = 671) were older adults, 72.28 (SD = 5.41) years old, (Black: n = 230; White, n = 441) diagnosed with heart failure and reduced ejection fraction. The model found perceived benefits, self-efficacy, perceived threats, and perceived barriers to predict exercise behavior among Black and White older adults. However, among these constructs, only perceived benefits and self-efficacy were facilitated via intervention for both races. Additionally, the intervention was effective for addressing perceived barriers to exercise only among White participants. Finally, the intervention did not result in a change of perceived threats for both races.

Conclusions

Among health belief model constructs, perceived threats and barriers were not facilitated for both races in the experimental arm, and the intervention did not resolve barriers among Black older adults. Racial differences need to be considered when designing interventions for clinical populations as future studies are warranted to address barriers to exercise among Black older adults with heart failure.
Surgical treatment of preauricular fistulas: a 12-year single-center clinical observation
Authors: Li Ke , Hao Yan , Zhao Jiuzhou , Zhou Leping , Wu Yongyan , Zeng Xianhai , Gao Wei , Zhang Xiangmin
Published: September 2023

Objective

This study aimed to assess the effects of surgical timing and approach on operative duration, postoperative suture removal time, and postoperative recurrence rate in the management of preauricular fistula. A 12-year single-center clinical observation was conducted to analyze the potential effects of different surgical strategies on these critical outcomes.

Methods

The clinical data from 576 (782 ears) patients who underwent surgical resection for preauricular fistulas were examined in this retrospective study. The patients were classified into various groups based on differences in operative duration, surgical techniques and the use of intraoperative magnifying equipment. Furthermore, the specific data on operative duration, postoperative suture removal time, and postoperative recurrence rate were also recorded.

Results

The average operative duration for 782 ears and the average time required for postoperative suture removal were determined to be (34.57 ± 4.25) min and (3.62 ± 0.76) days, respectively. Among the cases examined, recurrence occurred in 13 ears, but all of them were cured after a second surgery, resulting in a recurrence rate of 1.67% (13/782). Interestingly, the operative and postoperative suture removal time was prolonged during the infection period (P < 0.05). The postoperative recurrence rate was significantly higher in the absence of magnifying equipment, as compared to those with the use of a microscope with 2.5× magnification (P < 0.05). No statistically significant differences were noted in the recurrence rate when comparing different anesthesia methods and types of surgical incisions, as well as the intraoperative use of methylene blue, and partial removal of cartilage of the pedicle (P > 0.05).

Conclusion

The use of methylene blue, partial removal of the cartilage of the pedicle, and surgical incision during preauricular fistula resection did not affect the operative duration, postoperative suture removal time, and postoperative recurrence rate. Therefore, surgeons can select their preferred approaches based on their individual practices and patient-specific situations. However, the use of magnifying equipment during surgery is associated with a reduced risk of recurrence.
Active surveillance should be considered for select men with Grade Group 2 prostate cancer
Authors: Pekala Kelly R. , Bergengren Oskar , Eastham James A. , Carlsson Sigrid V.
Published: September 2023

Background

Treatment decisions for localized prostate cancer must balance patient preferences, oncologic risk, and preservation of sexual, urinary and bowel function. While Active Surveillance (AS) is the recommended option for men with Grade Group 1 (Gleason Score 3 + 3 = 6) prostate cancer without other intermediate-risk features, men with Grade Group 2 (Gleason Score 3 + 4 = 7) are typically recommended active treatment. For select patients, AS can be a possible initial management strategy for men with Grade Group 2. Herein, we review current urology guidelines and the urologic literature regarding recommendations and evidence for AS for this patient group.

Main body

AS benefits men with prostate cancer by maintaining their current quality of life and avoiding treatment side effects. AS protocols with close follow up always allow for an option to change course and pursue curative treatment. All the major guideline organizations now include Grade Group 2 disease with slightly differing definitions of eligibility based on risk using prostate-specific antigen (PSA) level, Gleason score, clinical stage, and other factors. Selected men with Grade Group 2 on AS have similar rates of deferred treatment and metastasis to men with Grade Group 1 on AS. There is a growing body of evidence from randomized controlled trials, large observational (prospective and retrospective) cohorts that confirm the oncologic safety of AS for these men. While some men will inevitably conclude AS at some point due to clinical reclassification with biopsy or imaging, some men may be able to stay on AS until transition to watchful waiting (WW). Magnetic resonance imaging is an important tool to confirm AS eligibility, to monitor progression and guide prostate biopsy.

Conclusion

AS is a viable initial management option for well-informed and select men with Grade Group 2 prostate cancer, low volume of pattern 4, and no other adverse clinicopathologic findings following a well-defined monitoring protocol. In the modern era of AS, urologists have tools at their disposal to better stage patients at initial diagnosis, risk stratify patients, and gain information on the biologic potential of a patient’s prostate cancer.
Diabetes knowledge and associated factors in adolescents and young adults with type 1 diabetes in Ouagadougou (Burkina Faso)
Authors: Zemba Daniel , Sagna Yempabou , Traore Solo , Zoungrana Lassane , Somé D. Patricia , Kissou S. Aimée , Guira Oumar , Yaméogo Téné Marceline
Published: September 2023

Background

Type 1 diabetes (T1D) in youth is becoming a public health problem in Sub-Saharan Africa, including Burkina Faso. However, little is known about the level of knowledge of these patients on T1D. This study aimed to evaluate the knowledge of diabetes in adolescents and young adults about the disease, and identify the factors associated in Burkina Faso.

Methods

A cross-sectional survey was conducted from April to June 2020 among youth with T1D, aged 10 to 30 years, and regularly followed in the internal medicine department of Yalgado Ouedraogo University Hospital of Ouagadougou, Burkina Faso. Data were collected using the French AJD (Aide aux Jeunes Diabétiques) validated diabetes knowledge and skills (DKS) questionnaire designed to test participants’ accuracy in knowledge about six different themes of T1D, as generalities of diabetes, hypoglycemia and hyperglycemia, management of insulin treatment, and self-monitoring blood glucose (SMBG). DKS level was determined by calculating the scores, and univariate and multivariate logistic regression were used to explore factors influencing DKS scores. This level was classified as insufficient or low ≤ 25/50, regular 26–39/50, and adequate or good ≥ 40/50.

Results

Sixty-three participants with a mean age of 19.05 years and a sex ratio (W/M) of 1.17 were included in our study. The mean HbA1c level was 9.79%, and 43 (68.23%) patients had an insufficient DKS level. The mean global DKS score of correct answers was 23.63/50. The percentage of correct answers was respectively 50% for the item “generalities of diabetes”, 32.4% for the item “hypoglycemia and hyperglycemia”, 67.72% for the item “diet”, 37.34% for the item “management of insulin treatment” and 44.97% for the item “SMBG”. In univariate analysis, a better patient DKS level was associated with university education and long duration of diabetes care follow-up (> 10 years, p < 0.05). Only increasing age remain associated with a better knowledge score (p < 0.05) in multivariate analysis.

Conclusion

This study is an important first step in identifying areas for intervention efforts about therapeutic education for youth with type I diabetes in Burkina Faso.
KIF15 missense variant is associated with the early onset of idiopathic pulmonary fibrosis
Authors: Hollmén Maria , Laaka Atte , Partanen Juulia J. , Koskela Jukka , Sutinen Eva , Kaarteenaho Riitta , Ainola Mari , Myllärniemi Marjukka
Published: September 2023

Background

Idiopathic pulmonary fibrosis (IPF) has an unknown aetiology and limited treatment options. A recent meta-analysis identified three novel causal variants in the TERT, SPDL1, and KIF15 genes. This observational study aimed to investigate whether the aforementioned variants cause clinical phenotypes in a well-characterised IPF cohort.

Methods

The study consisted of 138 patients with IPF who were diagnosed and treated at the Helsinki University Hospital and genotyped in the FinnGen FinnIPF study.

Data on > 25 clinical parameters were collected by two pulmonologists who were blinded to the genetic data for patients with TERT loss of function and missense variants, SPDL1 and KIF15 missense variants, and a MUC5B variant commonly present in patients with IPF, or no variants were separately analysed.

Results

The KIF15 missense variant is associated with the early onset of the disease, leading to progression to early-age transplantation or death. In patients with the KIF15 variant, the median age at diagnosis was 54.0 years (36.5–69.5 years) compared with 72.0 years (65.8–75.3 years) in the other patients (P = 0.023). The proportion of KIF15 variant carriers was 9- or 3.6-fold higher in patients aged < 55 or 65 years, respectively. The variants for TERT and MUC5B had similar effects on the patient’s clinical course, as previously described. No distinct phenotypes were observed in patients with the SPDL1 variant.

Conclusions

Our study indicated the potential of KIF15 to be used in the genetic diagnostics of IPF. Further studies are needed to elucidate the biological mechanisms of KIF15 in IPF.
Reducing the harms of xylazine: clinical approaches, research deficits, and public health context
Authors: Zagorski Claire M. , Hosey Rebecca A. , Moraff Christopher , Ferguson Aaron , Figgatt Mary , Aronowitz Shoshana , Stahl Natalie E. , Hill Lucas G. , McElligott Zoe , Dasgupta Nabarun
Published: September 2023

Objectives

Xylazine has emerged as a consistent part of the unregulated drug supply in recent months. We discuss major domains of xylazine’s harm, current knowledge deficits, clinical and harm reduction strategies for minimizing harm, and xylazine’s public health and policy context. As an interdisciplinary team from across the USA, we have pooled our knowledge to provide an overview of xylazine’s current and emerging contexts.

Methods

To inform this essay, the pertinent literature was reviewed, clinical knowledge and protocols were shared by multiple clinicians with direct expertise, and policy and public health context were added by expert authors.

Results

We describe xylazine’s major harm domains—acute poisoning, extended sedation, and wounds, along with anemia and hyperglycemia, which have been reported anecdotally but lack as clear of a connection to xylazine. Current successful practices for xylazine wound care are detailed. Understanding xylazine’s epidemiology will also require greater investment in drug checking and surveillance. Finally, approaches to community-based wound care are discussed, along with an orientation to the larger policy and public health context.

Conclusions

Addressing the harms of xylazine requires interdisciplinary participation, investment in community-based harm reduction strategies, and improved drug supply surveillance. The relatively unique context of xylazine demands buy-in from public health professionals, harm reduction professionals, clinicians, basic science researchers, policymakers and more.
Efficacy of virtual reality training on motor performance, activity of daily living, and quality of life in patients with Parkinson's disease: an umbrella review comprising meta-analyses of randomized controlled trials
Authors: Yu Jingxuan , Wu Jinlong , Lu Jiancong , Wei Xijun , Zheng Kangyong , Liu Bowen , Xiao Wen , Shi Qiuqiong , Xiong Lilin , Ren Zhanbing
Published: September 2023

Objective

There are several meta-analyses of randomized controlled trials (RCTs) demonstrating the benefits of virtual reality (VR) training as an intervention for motor performance, activity of daily living (ADL) and quality of life (QoL) outcomes in patients with Parkinson's disease (PD). However, the aggregate evidence collected to date has not been thoroughly evaluated for strength, quality, and reproducibility. An umbrella review from published meta-analyses of RCTs was conducted to evaluate the strength and quality of existing evidence regarding the efficacy of VR training in improving the motor performance, ADL and QoL outcomes of patients with PD.

Methods

PubMed, PsychInfo, Web of Science, and Scopus were searched to identify relevant meta-analysis of RCTs examining the effects of VR training on motor performance and quality of life outcomes in PD patients. We recalculated the effect sizes (Hedges’g) for VR training using DerSimonian and Laird (DL) random effects models. We further assessed between-study heterogeneity, prediction interval (PI), publication bias, small-size studies, and whether the results of the observed positive studies were better than would be expected by chance. Based on these calculations, the quality of evidence for each outcome was assessed by using the Grading of Recommendations, Assessment, Development, and Evaluations (GRADE) criteria.

Results

Four meta-analysis with eight outcomes included in the umbrella review was recalculated effect size. Pooled results found VR training can large improve the basic balance ability, moderate improve the overall balance capacity and moderate improve the stride length in PD patients. For ADL and QoL, the effect sizes were pooled that suggested VR training can moderate improve ADL and QoL for PD patients. However, no statistically clear evidence was found in walking speed, motor function and gait function during VR training. The analyzed meta-analyses showed low-to-moderate methodological quality (AMSTAR2) as well as presented evidence of moderate-to-very low quality (GRADE). Tow adverse reactions were reported in the included meta-analyses.

Conclusions

In this umbrella review, a beneficial correlation between VR and balance ability, stride length, ADL and QoL in PD patients was discovered, especially for the very positive effect of VR on balance because of two of the eight outcomes related to balance ability showed large effect size. The observations were accompanied by moderate- to very low-quality rating evidence, supporting VR training as a practical approach to rehabilitation.
ATR-binding lncRNA ScaRNA2 promotes cancer resistance through facilitating efficient DNA end resection during homologous recombination repair
Authors: Chen Yuanyuan , Shen Hui , Liu Tingting , Cao Kun , Wan Zhijie , Du Zhipeng , Wang Hang , Yu Yue , Ma Shengzhe , Lu Edward , Zhang Wei , Cai Jianming , Gao Fu , Yang Yanyong
Published: September 2023

Background

Our previous study first showed that ATR-binding long noncoding RNA (lncRNA) is necessary for ATR function and promotes cancer resistance. However, the specific lncRNAs instrumental in ATR activation remain largely unclear, which limits our comprehensive understanding of this critical biological process.

Methods

RNA immunoprecipitation (RIP) followed by RNA sequencing was employed to identify ATR-binding lncRNAs, which were further validated using RIP-qPCR assays. Immunofluorescence staining and Western blotting were applied to detect the activation of DNA damage repair factors. After the effect of scaRNA2 on cellular sensitivity to DNA-damaging reagents was determined, the effects of scaRNA2 on radiotherapy were investigated in patient-derived organoids and xenograft preclinical models. The clinical relevance of scaRNA2 was also validated in tissues isolated from rectal cancer patients.

Results

ScaRNA2 was identified as the most enriched ATR-binding lncRNA and was found to be essential for homologous recombination (HR) mediated DNA damage repair. Furthermore, scaRNA2 knockdown abrogated the recruitment of ATR and its substrates in response to DNA damage. Mechanistically, scaRNA2 was observed to be necessary for Exo1-mediated DNA end resection and bridged the MRN complex to ATR activation. Knockdown of scaRNA2 effectively increased the sensitivity of cancer cells to multiple kinds of DNA damage-related chemoradiotherapy. Preclinically, knockdown of scaRNA2 improved the effects of radiotherapy on patient-derived organoids and xenograft models. Finally, an increase in scaRNA2 colocalized with ATR was also found in clinical patients who were resistant to radiotherapy.

Conclusions

ScaRNA2 was identified as the most abundant lncRNA bound to ATR and was demonstrated to bridge DNA end resection to ATR activation; thus, it could be applied as a potent target for combined cancer treatments with chemoradiotherapy.
Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) alleviate excessive autophagy of ovarian granular cells through VEGFA/PI3K/AKT/mTOR pathway in premature ovarian failure rat model
Authors: Dai Wenjie , Yang Hong , Xu Bo , He Tiantian , Liu Ling , Ma Xiaoqian , Ma Jiaxue , Yang Guoqin , Si Rui , Pei Xiuying , Du Xing , Fu Xufeng
Published: September 2023

Background

Premature ovarian failure (POF) is one of the leading causes of female infertility and is accompanied by abnormal endocrine, seriously affecting female quality of life. Previous studies have demonstrated that mesenchymal stem cells (MSCs) transplantation is a promising therapeutic strategy for POF. However, the mechanism remains obscure. This study aims to investigate the therapeutic effect of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on ovarian function in the POF rat model and explore the underlying mechanisms.

Methods

The ovarian function was evaluated by ovarian morphology, histology, estrous cycle, hormone levels (AMH, E2, FSH, and LH), and fertility ability to investigate the effect of hUC-MSCs on the POF rats model. The cytokines levels were assayed in serum using protein array to explore the mechanisms of hUC-MSCs therapy for POF. The excessive autophagy levels were evaluated using a co-culture system of 3D MSCs spheroids with human ovarian granulosa cell line (KGN) or primary ovarian granulosa cells (GCs) to understand the paracrine effect of hUC-MSCs on GCs. The related proteins expression of autophagy and PI3K/AKT/mTOR pathway was detected using Western Blotting and/or in various inhibitors supplement to further demonstrate that vascular endothelial growth factor A (VEGFA) secreted by hUC-MSCs can alleviate excessive autophagy of ovarian GCs via PI3K/AKT/mTOR signaling pathway. The ovarian culture model in vitro was applied to confirm the mechanism.

Results

The ovarian function of POF and the excessive autophagy of ovarian GCs were restored after hUC-MSCs transplantation. The protein array result demonstrated that VEGF and PI3K/AKT might improve ovarian function. in vitro experiments demonstrated that VEGFA secreted by hUC-MSCs could decrease oxidative stress and inhibit excessive autophagy of ovarian GCs via PI3K/AKT/mTOR pathway. The ovarian culture model results confirmed this mechanism in vitro.

Conclusion

The hUC-MSCs can alleviate excessive autophagy of ovarian GCs via paracrine VEGFA and regulate the PI3K/AKT/mTOR signaling pathway, thereby improving the ovarian function of POF.
Time definition of reintubation most relevant to patient outcomes in critically ill patients: a multicenter cohort study
Authors: Tanaka Aiko , Shimomura Yoshimitsu , Uchiyama Akinori , Tokuhira Natsuko , Kitamura Tetsuhisa , Iwata Hirofumi , Hashimoto Haruka , Ishigaki Suguru , Enokidani Yusuke , Yamashita Tomonori , Koyama Yukiko , Iguchi Naoya , Yoshida Takeshi , Fujino Yuji
Published: September 2023

Background

Reintubation is a common complication in critically ill patients requiring mechanical ventilation. Although reintubation has been demonstrated to be associated with patient outcomes, its time definition varies widely among guidelines and in the literature. This study aimed to determine the association between reintubation and patient outcomes as well as the consequences of the time elapsed between extubation and reintubation on patient outcomes.

Methods

This was a multicenter retrospective cohort study of critically ill patients conducted between April 2015 and March 2021. Adult patients who underwent mechanical ventilation and extubation in intensive care units (ICUs) were investigated utilizing the Japanese Intensive Care PAtient Database. The primary and secondary outcomes were in-hospital and ICU mortality. The association between reintubation and clinical outcomes was studied using Cox proportional hazards analysis. Among the patients who underwent reintubation, a Cox proportional hazard analysis was conducted to evaluate patient outcomes according to the number of days from extubation to reintubation.

Results

Overall, 184,705 patients in 75 ICUs were screened, and 1849 patients underwent reintubation among 48,082 extubated patients. After adjustment for potential confounders, multivariable analysis revealed a significant association between reintubation and increased in-hospital and ICU mortality (adjusted hazard ratio [HR] 1.520, 95% confidence interval [CI] 1.359–1.700, and adjusted HR 1.325, 95% CI 1.076–1.633, respectively). Among the reintubated patients, 1037 (56.1%) were reintubated within 24 h after extubation, 418 (22.6%) at 24–48 h, 198 (10.7%) at 48–72 h, 111 (6.0%) at 72–96 h, and 85 (4.6%) at 96–120 h. Multivariable Cox proportional hazard analysis showed that in-hospital and ICU mortality was highest in patients reintubated at 72–96 h (adjusted HR 1.528, 95% CI 1.062–2.197, and adjusted HR 1.334, 95% CI 0.756–2.352, respectively; referenced to reintubation within 24 h).

Conclusions

Reintubation was associated with a significant increase in in-hospital and ICU mortality. The highest mortality rates were observed in patients who were reintubated between 72 and 96 h after extubation. Further studies are warranted for the optimal observation of extubated patients in clinical practice and to strengthen the evidence for mechanical ventilation.
Fabrication of an electrochemical sensor based on eggshell waste recycling for the voltammetric simultaneous detection of the antibiotics ofloxacin and ciprofloxacin
Authors: Khodari M. , Assaf H. F. , Shamroukh Ahmed A. , Rabie E. M.
Published: September 2023

In this work, an accurate, highly sensitive, and economical electrochemical sensor based on a carbon paste electrode modified by Ca2CuO3 nanostructure (Ca2CuO3 NS) was constructed using Eggshell waste recycling as a cheap source of calcium. The Ca2CuO3 NS was analyzed using FTIR, SEM, and XRD measurements. The synthesized nanomaterials utilized for the first time to enhance the electrocatalytic efficiency of carbon paste electrode (CPE) toward fluoroquinolones antibiotics ofloxacin (OFL) and ciprofloxacin (CIP), The drugs used to treat pneumonia caused by COVID-19. The synthesized Ca2CuO3 NS dramatically enhanced the anodic peak response of CPE toward both drugs compared to the unmodified one and other modified electrodes. The simultaneous detection of the two antibiotics was performed in the linear range of 0.09–1.0 μM for OFL and 0.05–0.8 μM for CIP with the LOD of 0.027 μM and 0.012 μM, respectively. The suggested method was applied successfully to determine OFL and CIP in real samples.
A DNA methylation signature in the stress driver gene Fkbp5 indicates a neuropathic component in chronic pain
Authors: Maiarù Maria , Acton Richard J. , Woźniak Eva L. , Mein Charles A. , Bell Christopher G. , Géranton Sandrine M.
Published: September 2023

Background

Epigenetic changes can bring insight into gene regulatory mechanisms associated with disease pathogenicity, including chronicity and increased vulnerability. To date, we are yet to identify genes sensitive to epigenetic regulation that contribute to the maintenance of chronic pain and with an epigenetic landscape indicative of the susceptibility to persistent pain. Such genes would provide a novel opportunity for better pain management, as their epigenetic profile could be targeted for the treatment of chronic pain or used as an indication of vulnerability for prevention strategies. Here, we investigated the epigenetic profile of the gene Fkbp5 for this potential, using targeted bisulphite sequencing in rodent pre-clinical models of chronic and latent hypersensitive states.

Results

The Fkbp5 promoter DNA methylation (DNAm) signature in the CNS was significantly different between models of persistent pain, and there was a significant correlation between CNS and peripheral blood Fkbp5 DNAm, indicating that further exploration of Fkbp5 promoter DNAm as an indicator of chronic pain pathogenic origin is warranted. We also found that maternal separation, which promotes the persistency of inflammatory pain in adulthood, was accompanied by long-lasting reduction in Fkbp5 DNAm, suggesting that Fkbp5 DNAm profile may indicate the increased vulnerability to chronic pain in individuals exposed to trauma in early life.

Conclusions

Overall, our data demonstrate that the Fkbp5 promoter DNAm landscape brings novel insight into the differing pathogenic origins of chronic pain, may be able to stratify patients and predict the susceptibility to chronic pain.
Deoxyguanosine kinase deficiency and recurrent spontaneous pneumothorax: a case report
Authors: Ramboux Alice , Poncelet Alain , Clapuyt Philippe , Scheers Isabelle , Sokal Etienne , Reding Raymond , Stephenne Xavier
Published: September 2023

Background

Deoxyguanosine kinase deficiency is mainly manifested by hepatic and neurological damage, hence it belongs to the hepatocerebral form of mitochondrial deoxyribonucleic acid depletion syndrome. The association between deoxyguanosine kinase deficiency and recurrent spontaneous pneumothorax has not currently been reported.

Case presentation

A 12-year-old Russian boy with deoxyguanosine kinase deficiency, a recipient of a liver transplant with amyotrophy secondary to his mitochondriopathy, presented with recurrent spontaneous bilateral pneumothorax refractory to drainage and surgery.

Conclusion

To our knowledge, this is the first documented case of deoxyguanosine kinase deficiency associated with recurrent spontaneous pneumothorax, which could be considered a late complication of deoxyguanosine kinase deficiency. At this point, this is only an association and further studies and research need to be performed to help confirm the pathogenesis of this association.
NADH elevation during chronic hypoxia leads to VHL-mediated HIF-1α degradation via SIRT1 inhibition
Authors: Joo Hyun-Yoo , Jung Jin Kyu , Kim Mi-Yeon , Woo Seon Rang , Jeong Jae Min , Park Eun-Ran , Kim Yong-Min , Park Joong-Jean , Kim Joon , Yun Miyong , Shin Hyun-Jin , Lee Kee-Ho
Published: September 2023

Background

Under conditions of hypoxia, cancer cells with hypoxia inducible factor-1α (HIF-1α) from heterogeneous tumor cells show greater aggression and progression in an effort to compensate for harsh environmental conditions. Extensive study on the stability of HIF-1α under conditions of acute hypoxia in cancer progression has been conducted, however, understanding of its involvement during the chronic phase is limited.

Methods

In this study, we investigated the effect of SIRT1 on HIF1 stability in a typical chronic hypoxic conditon that maintains cells for 24 h under hypoxia using Western blotting, co-IP, measurement of intracellular NAD + and NADH levels, semi-quantitative RT-PCR analysis, invasion assay, gene knockdown.

Results

Here we demonstrated that the high concentration of pyruvate in the medium, which can be easily overlooked, has an effect on the stability of HIF-1α. We also demonstrated that NADH functions as a signal for conveyance of HIF-1α degradation via the SIRT1 and VHL signaling pathway under conditions of chronic hypoxia, which in turn leads to attenuation of hypoxically strengthened invasion and angiogenic activities. A steep increase in the level of NADH occurs during chronic hypoxia, leading to upregulation of acetylation and degradation of HIF-1α via inactivation of SIRT1. Of particular interest, p300-mediated acetylation at lysine 709 of HIF-1α is recogonized by VHL, which leads to degradation of HIF-1α via ubiquitin/proteasome machinary under conditions of chronic hypoxia. In addition, we demonstrated that NADH-elevation-induced acetylation and subsequent degradation of HIF-1α was independent of proline hydroxylation.

Conclusions

Our findings suggest a critical role of SIRT1 as a metabolic sensor in coordination of hypoxic status via regulation of HIF-1α stability. These results also demonstrate the involvement of VHL in degradation of HIF-1α through recognition of PHD-mediated hydroxylation in normoxia and p300-mediated HIF-1α acetylation in hypoxia.