bluuebunny/crossref_metadata_2025 huggingface dataset:
INFLATION, LIQUIDITY, AND LONG-RUN GROWTH
Authors: Zhou Ge
Published: December 2016

This paper revisits the long-run relationship between inflation and economic growth by exploring the impact of inflation on investment. I illustrate that inflation may have a positive effect on growth by mitigating the liquidity risks of investment projects. Together with the traditional effect of the “inflation tax” on investment, a hump-shaped relationship between inflation and economic growth can be obtained in a calibrated model, which is consistent with the US postwar data. Sensitivity analysis suggests that the degree of financial development and the magnitude of the aggregate liquidity demand help explain the mixed empirical findings.
Introduction of complementary foods in Sweden and impact of maternal education on feeding practices
Authors: Klingberg Sofia , Ludvigsson Johnny , Brekke Hilde K
Published: December 2016

Objective

To describe the introduction of complementary foods in a population-based cohort in relation to recommendations and explore the possible impact of maternal education on infant feeding practices.

Design

Prospective data from the All Babies in Southeast Sweden (ABIS) cohort study were used. The ABIS study invited all infants born in south-east Sweden during October 1997–October 1999 (n 21 700) to participate. A questionnaire was completed for 16 022 infants. During the infants’ first year parents continuously filed in a diary covering introduction of foods.

Setting

Sweden.

Subjects

Infants (n 9727) with completed food diaries.

Results

Potatoes, vegetables, fruits/berries and porridge were the foods first introduced, with a median introduction between 19 and 22 weeks, followed by introduction of meat, cow’s milk, follow-on formula and sour milk/yoghurt between 24 and 27 weeks. Early introduction of any food, before 16 weeks, occurred for 27 % of the infants and was more common in infants of mothers with low education. Overall, potatoes (14·7 %), vegetables (11·1 %), fruits/berries (8·5 %), porridge (7·4 %) and follow-on formula (2·7 %) were the foods most frequently introduced early. The majority of infants (≥70 %) were introduced to potatoes, vegetables, fruits/berries and porridge during concurrent breast-feeding, but introduction during concurrent breast-feeding was less common in infants of mothers with low education.

Conclusions

Most infants were introduced to complementary foods timely in relation to recommendations. Low maternal education was associated with earlier introduction of complementary foods and less introduction during concurrent breast-feeding. Still, the results indicated exposure to fewer foods at 12 months in infants of mothers with low education.
Context-specific complementary feeding recommendations developed using Optifood could improve the diets of breast-fed infants and young children from diverse livelihood groups in northern Kenya
Authors: Vossenaar Marieke , Knight Frances A , Tumilowicz Alison , Hotz Christine , Chege Peter , Ferguson Elaine L
Published: December 2016

Objective

To formulate age- and context-specific complementary feeding recommendations (CFR) for infants and young children (IYC) and to compare the potential of filling population-level nutrient gaps using common sets of CFR across age groups.

Design

Linear programming was used to develop CFR using locally available and acceptable foods based on livelihood- and age-group-specific dietary patterns observed through 24 h dietary recalls. Within each livelihood group, the nutrient potential of age-group-specific v. consolidated CFR across the three age groups was tested.

Setting

Three food-insecure counties in northern Kenya; namely, settled communities from Isiolo (n 300), pastoralist communities from Marsabit (n 283) and agro-pastoralist communities from Turkana (n 299).

Subjects

Breast-fed IYC aged 6–23 months (n 882).

Results

Age-specific CFR could achieve adequacy for seven to nine of eleven modelled micronutrients, except among 12–23-month-old children in agro-pastoralist communities. Contribution of Fe, Zn and niacin remained low for most groups, and thiamin, vitamin B6 and folate for some groups. Age-group-consolidated CFR could not reach the same level of nutrient adequacy as age-specific sets among the settled and pastoralist communities.

Conclusions

Context- and age-specific CFR could ensure adequate levels of more modelled nutrients among settled and pastoralist IYC than among agro-pastoralist communities where use of nutrient-dense foods was limited. Adequacy of all eleven modelled micronutrients was not achievable and additional approaches to ensure adequate diets are required. Consolidated messages should be easier to implement as part of a behaviour change strategy; however, they would likely not achieve the same improvements in population-level dietary adequacy as age-specific CFR.
Insomnia in breast cancer: Independent symptom or symptom cluster?
Authors: Gehrman Philip R. , Garland Sheila N. , Matura Lea Ann , Mao Jun
Published: December 2016

Objective:

This study examined insomnia in the context of breast cancer, both as an independent symptom and as a component of a symptom cluster that includes depression, anxiety, fatigue, and pain.

Method:

Women with a history of breast cancer currently taking an aromatase inhibitor and who had completed cancer treatment at least one month prior to enrollment were included (n = 413). Participants completed validated measures of insomnia, fatigue, pain, depression, and anxiety. Factor analysis was utilized to examine the extent to which these symptoms could be represented by common latent factors. Insomnia severity was then separated into a symptom cluster component (I–SC) and an insomnia-unique (I–U) component. The associations between each insomnia component and demographic and clinical factors were examined in multivariate models.

Results:

A single-factor solution provided the best fit to the symptom measures. Some 53.3% of the variance in insomnia severity was captured by this symptom cluster (I–SC), with the remaining 43.7% being unique to insomnia (I–U). Unique patterns of demographic factors (e.g., age and body–mass index), but not clinical factors, were associated with each insomnia measure.

Significance of results:

Approximately 50% of insomnia severity was related to the symptom cluster, with the rest being unique to insomnia. Different sociodemographic risk factors were related to the different insomnia measures. Stronger underlying foundations for the mechanisms of each component could lead to refined diagnoses and targeted interventions for addressing the overall insomnia burden in cancer patients.
No-till snap bean performance and weed response following rye and vetch cover crops
Authors: Boydston Rick A. , Williams Martin M.
Published: December 2016

Fall-planted cover crops offer many benefits including weed suppressive residues in spring sown crops when controlled and left on the soil surface. However, vegetable growers have been slow to adopt direct-seeding (no-till) into cover crop residues. Field studies were conducted in 2009 and 2010 near Paterson, WA and Urbana, IL to evaluate mortality of rye and common vetch (WA) hairy vetch (IL) cover crops, weed density and biomass, and snap bean growth and yield following four cover crop control methods utilizing a roller–crimper. Rye had higher mortality than common and hairy vetch by roller-crimping, and carfentrazone applied after roller crimping only slightly increased vetch mortality. Heavy residues of rye and escaped vetch were difficult to plant into, often resulting in lower snap bean populations. Rye and hairy vetch residues suppressed final weed biomass, while common vetch reduced weed biomass 1 of 2 years. Escaped plants of both vetch species became a weed. Snap bean yields were inconsistent and often lower following cover crops compared with a fallow treatment. Being able to completely control cover crops and to plant, manage escaped weeds and mechanically harvest in the presence of heavy residues are challenges that deter vegetable growers from readily adopting these systems.
The Measurement of Intelligence in the XXI Century using Video Games
Authors: Quiroga M. A. , Román F. J. , De La Fuente J. , Privado J. , Colom R.
Published: December 2016

This paper reviews the use of video games for measuring intelligence differences and reports two studies analyzing the relationship between intelligence and performance on a leisure video game. In the first study, the main focus was to design an Intelligence Test using puzzles from the video game. Forty-seven young participants played “Professor Layton and the curious village”® for a maximum of 15 hours and completed a set of intelligence standardized tests. Results show that the time required for completing the game interacts with intelligence differences: the higher the intelligence, the lower the time (d = .91). Furthermore, a set of 41 puzzles showed excellent psychometric properties. The second study, done seven years later, confirmed the previous findings. We finally discuss the pros and cons of video games as tools for measuring cognitive abilities with commercial video games, underscoring that psychologists must develop their own intelligence video games and delineate their key features for the measurement devices of next generation.
Less is More in Hong Kong: Investigation of Biscriptal and Trilingual Development Among Chinese Twins in a (Relatively) Small City
Authors: Wong Simpson W. L. , Ho Connie Suk-Han , McBride Catherine , Chow Bonnie Wing-Yin , Waye Mary Miu Yee
Published: December 2016

One salient characteristic of twin studies and the related behavioral genetics paradigm is the requirement of a large sample size. Countries or regions that are large in size and highly populated are at an advantage when implementing twin studies. However, given the fascinating and promising results obtained from twin studies, many researchers based in smaller countries or regions may still want to conduct twin studies in order to address local and theoretical issues. In this article, we have outlined the development of twin studies in Hong Kong, one of the Special Administrative Regions of China. The historical development and design of the two major twin studies of language and reading development implemented within Hong Kong are discussed, providing insights to researchers who also aspire to conduct twin studies in small regions.
Using Conditional Association to Identify Locally Independent Item	Sets
Authors: Straat J. Hendrik , van der Ark L. Andries , Sijtsma Klaas
Published: December 2016

Abstract. The ordinal, unidimensional monotone latent variable model assumes unidimensionality, local independence, and monotonicity, and implies the observable property of conditional association. We investigated three special cases of conditional association and implemented them in a new procedure that aims at identifying locally dependent items, removing these items from the initial item set, and producing an item subset that is locally independent. A simulation study showed that the new procedure correctly identified 89.5% of the model-consistent items and up to 90% of the model-inconsistent items. We recommend using this procedure for selecting locally independent item sets. The procedure may be used in combination with Mokken scale analysis.
Dealing With Data Streams
Authors: Ippel Lianne , Kaptein Maurits , Vermunt Jeroen
Published: December 2016

Abstract. Novel technological advances allow distributed and automatic measurement of human behavior. While these technologies provide exciting new research opportunities, they also provide challenges: datasets collected using new technologies grow increasingly large, and in many applications the collected data are continuously augmented. These data streams make the standard computation of well-known estimators inefficient as the computation has to be repeated each time a new data point enters. In this tutorial paper, we detail online learning, an analysis method that facilitates the efficient analysis of Big Data and continuous data streams. We illustrate how common analysis methods can be adapted for use with Big Data using an online, or “row-by-row,” processing approach. We present several simple (and exact) examples of the online estimation and discuss Stochastic Gradient Descent as a general (approximate) approach to estimate more complex models. We end this article with a discussion of the methodological challenges that remain.
OTX2 impedes self–renewal of porcine iPS cells through downregulation of NANOG expression
Authors: Wang Ning , Wang Yaxian , Xie Youlong , Wang Huayan
Published: December 2016

The transcription factor Otx2 acts as a negative switch in the regulation of transition from naive to primed pluripotency in mouse pluripotent stem cells. However, the molecular features and function of porcine OTX2 have not been well elucidated in porcine-induced pluripotent stem cells (piPSCs). By studying high-throughput transcriptome sequencing and interfering endogenous OTX2 expression, we demonstrate that OTX2 is able to downgrade the self-renewal of piPSCs. OTX2 is highly expressed in porcine brain, reproductive tissues, and preimplantation embryos, but is undetectable in fibroblasts and most somatic tissues. However, the known piPSC lines reported previously produced different levels of OTX2 depending on the induction procedures and culture conditions. Overexpression of porcine OTX2 can reduce the percentage of alkaline phosphatase-positive colonies and downregulate NANOG and OCT4 expression. In contrast, knockdown of OTX2 can significantly increase endogenous expressions of NANOG, OCT4, and ESRRB, and stabilize the pluripotent state of piPSCs. On the other hand, NANOG can directly bind to the OTX2 promoter as shown in ChIP-seq data and repress OTX2 promoter activity in a dose-dependent manner. These observations indicate that OTX2 and NANOG can form a negative feedback circuitry to regulate the pluripotency of porcine iPS cells.
Real-time mechanical characterization of DNA degradation under therapeutic X-rays and its theoretical modeling
Authors: Perret Grégoire , Lacornerie Thomas , Manca Fabio , Giordano Stefano , Kumemura Momoko , Lafitte Nicolas , Jalabert Laurent , Tarhan Mehmet C. , Lartigau Eric F. , Cleri Fabrizio , Fujita Hiroyuki , Collard Dominique
Published: December 2016

The killing of tumor cells by ionizing radiation beams in cancer radiotherapy is currently based on a rather empirical understanding of the basic mechanisms and effectiveness of DNA damage by radiation. By contrast, the mechanical behaviour of DNA encompassing sequence sensitivity and elastic transitions to plastic responses is much better understood. A novel approach is proposed here based on a micromechanical Silicon Nanotweezers device. This instrument allows the detailed biomechanical characterization of a DNA bundle exposed to an ionizing radiation beam delivered here by a therapeutic linear particle accelerator (LINAC). The micromechanical device endures the harsh environment of radiation beams and still retains molecular-level detection accuracy. In this study, the first real-time observation of DNA damage by ionizing radiation is demonstrated. The DNA bundle degradation is detected by the micromechanical device as a reduction of the bundle stiffness, and a theoretical model provides an interpretation of the results. These first real-time observations pave the way for both fundamental and clinical studies of DNA degradation mechanisms under ionizing radiation for improved tumor treatment.
Towards uniformly oriented diatom frustule monolayers: Experimental and theoretical analyses
Authors: Li Aobo , Zhang Wenqiang , Ghaffarivardavagh Reza , Wang Xiaoning , Anderson Stephan W. , Zhang Xin
Published: December 2016

Diatoms are unicellular, photosynthetic algae that are ubiquitous in aquatic environments. Their unique, three-dimensional (3D) structured silica exoskeletons, also known as frustules, have drawn attention from a variety of research fields due to their extraordinary mechanical properties, enormous surface area, and unique optical properties. Despite their promising use in a range of applications, without methods to uniformly control the frustules’ alignment/orientation, their full potential in technology development cannot be realized. In this paper, we realized and subsequently modeled a simple bubbling method for achieving large-area, uniformly oriented Coscinodiscus species diatom frustules. With the aid of bubble-induced agitations, close-packed frustule monolayers were achieved on the water–air interface with up to nearly 90% of frustules achieving uniform orientation. The interactions between bubble-induced agitations were modeled and analyzed, demonstrating frustule submersion and an adjustment of the orientation during the subsequent rise towards the water’s surface to be fundamental to the experimentally observed uniformity. The method described in this study holds great potential for frustules’ engineering applications in a variety of technologies, from sensors to energy-harvesting devices.
hMYH and hMTH1 cooperate for survival in mismatch repair defective T-cell acute lymphoblastic leukemia
Authors: Eshtad S , Mavajian Z , Rudd S G , Visnes T , Boström J , Altun M , Helleday T
Published: December 2016

hMTH1 is an 8-oxodGTPase that prevents mis-incorporation of free oxidized nucleotides into genomic DNA. Base excision and mismatch repair pathways also restrict the accumulation of oxidized lesions in DNA by removing the mis-inserted 8-oxo-7,8-dihydro-2'-deoxyguanosines (8-oxodGs). In this study, we aimed to investigate the interplay between hMYH DNA glycosylase and hMTH1 for cancer cell survival by using mismatch repair defective T-cell acute lymphoblastic leukemia (T-ALL) cells. To this end, MYH and MTH1 were silenced individually or simultaneously using small hairpin RNAs. Increased sub-G1 population and apoptotic cells were observed upon concurrent depletion of both enzymes. Elevated cell death was consistent with cleaved caspase 3 accumulation in double knockdown cells. Importantly, overexpression of the nuclear isoform of hMYH could remove the G1 arrest and partially rescue the toxicity observed in hMTH1-depleted cells. In addition, expression profiles of human DNA glycosylases were generated using quantitative reverse transcriptase–PCR in MTH1 and/or MYH knockdown cells. NEIL1 DNA glycosylase, involved in repair of oxidized nucleosides, was found to be significantly downregulated as a cellular response to MTH1–MYH co-suppression. Overall, the results suggest that hMYH and hMTH1 functionally cooperate for effective repair and survival in mismatch repair defective T-ALL Jurkat A3 cells.
Sharing brain mapping statistical results with the neuroimaging data model
Authors: Maumet Camille , Auer Tibor , Bowring Alexander , Chen Gang , Das Samir , Flandin Guillaume , Ghosh Satrajit , Glatard Tristan , Gorgolewski Krzysztof J. , Helmer Karl G. , Jenkinson Mark , Keator David B. , Nichols B. Nolan , Poline Jean-Baptiste , Reynolds Richard , Sochat Vanessa , Turner Jessica , Nichols Thomas E.
Published: December 2016

Only a tiny fraction of the data and metadata produced by an fMRI study is finally conveyed to the community. This lack of transparency not only hinders the reproducibility of neuroimaging results but also impairs future meta-analyses. In this work we introduce NIDM-Results, a format specification providing a machine-readable description of neuroimaging statistical results along with key image data summarising the experiment. NIDM-Results provides a unified representation of mass univariate analyses including a level of detail consistent with available best practices. This standardized representation allows authors to relay methods and results in a platform-independent regularized format that is not tied to a particular neuroimaging software package. Tools are available to export NIDM-Result graphs and associated files from the widely used SPM and FSL software packages, and the NeuroVault repository can import NIDM-Results archives. The specification is publically available at: http://nidm.nidash.org/specs/nidm-results.html.
Distributed solar photovoltaic array location and extent dataset for remote sensing object identification
Authors: Bradbury Kyle , Saboo Raghav , L. Johnson Timothy , Malof Jordan M. , Devarajan Arjun , Zhang Wuming , M. Collins Leslie , G. Newell Richard
Published: December 2016

Earth-observing remote sensing data, including aerial photography and satellite imagery, offer a snapshot of the world from which we can learn about the state of natural resources and the built environment. The components of energy systems that are visible from above can be automatically assessed with these remote sensing data when processed with machine learning methods. Here, we focus on the information gap in distributed solar photovoltaic (PV) arrays, of which there is limited public data on solar PV deployments at small geographic scales. We created a dataset of solar PV arrays to initiate and develop the process of automatically identifying solar PV locations using remote sensing imagery. This dataset contains the geospatial coordinates and border vertices for over 19,000 solar panels across 601 high-resolution images from four cities in California. Dataset applications include training object detection and other machine learning algorithms that use remote sensing imagery, developing specific algorithms for predictive detection of distributed PV systems, estimating installed PV capacity, and analysis of the socioeconomic correlates of PV deployment.
A collection of European sweet cherry phenology data for assessing climate change
Authors: Wenden Bénédicte , Campoy José Antonio , Lecourt Julien , López Ortega Gregorio , Blanke Michael , Radičević Sanja , Schüller Elisabeth , Spornberger Andreas , Christen Danilo , Magein Hugo , Giovannini Daniela , Campillo Carlos , Malchev Svetoslav , Peris José Miguel , Meland Mekjell , Stehr Rolf , Charlot Gérard , Quero-García José
Published: December 2016

Professional and scientific networks built around the production of sweet cherry (Prunus avium L.) led to the collection of phenology data for a wide range of cultivars grown in experimental sites characterized by highly contrasted climatic conditions. We present a dataset of flowering and maturity dates, recorded each year for one tree when available, or the average of several trees for each cultivar, over a period of 37 years (1978–2015). Such a dataset is extremely valuable for characterizing the phenological response to climate change, and the plasticity of the different cultivars’ behaviour under different environmental conditions. In addition, this dataset will support the development of predictive models for sweet cherry phenology exploitable at the continental scale, and will help anticipate breeding strategies in order to maintain and improve sweet cherry production in Europe.
Ascites’ neutrophil function is significantly impaired in patients with decompensated cirrhosis but can be restored by autologous plasma incubation
Authors: Engelmann Cornelius , Becker Christina , Boldt Andreas , Herta Toni , Boehlig Albrecht , Splith Katrin , Schmelzle Moritz , Mueller Niklas , Krohn Sandra , Tautenhahn Hans-Michael , Bartels Michael , Sack Ulrich , Berg Thomas
Published: December 2016

Systemic immune cell dysfunction is a typical feature of liver diseases and increases the risk of bacterial infection, especially spontaneous bacterial peritonitis. We evaluated functional properties of neutrophil granulocytes in blood and ascites of patients both with and without decompensated cirrhosis. We collected blood and ascites samples from 63 patients with cirrhosis and eight without cirrhosis. Phagocytosis activity (PA) and oxidative burst activity (OBA) were evaluated after ex vivo stimulation with E. coli, while fluorescence signals were measured by flow cytometry. Ascites’ neutrophil function tests were repeated after incubation with autologous plasma. Ascites’ neutrophils showed an impaired PA and OBA (median blood PA 98.1% (86.8–99.8) vs. ascites’ PA 50.5% (0.4–97.3), p < 0.0001; median blood OBA 98.7% (27.5–100) vs. ascites’ OBA 27.5% (0.3–96.7), p < 0.0001). Patients with non-cirrhotic ascites showed higher PA but equally suppressed OBA. Ascites’ neutrophil function could be partially restored after incubation with autologous plasma (median increase PA: 22.5% (−49.7 – +93.2), p = 0.002; OBA: 22.8% (−10.4 – +48.8), p = 0.002). Ascites’ neutrophils of patients with cirrhosis are functionally impaired, but could be partially restored after incubation with plasma. Further investigations are needed to identify the factors in ascites that are associated with neutrophils’ function.
A network intervention that locates and intervenes with recently HIV-infected persons: The Transmission Reduction Intervention Project (TRIP)
Authors: Nikolopoulos Georgios K. , Pavlitina Eirini , Muth Stephen Q. , Schneider John , Psichogiou Mina , Williams Leslie D. , Paraskevis Dimitrios , Sypsa Vana , Magiorkinis Gkikas , Smyrnov Pavlo , Korobchuk Anya , Vasylyeva Tetyana I. , Skaathun Britt , Malliori Melpomeni , Kafetzopoulos Evangelos , Hatzakis Angelos , Friedman Samuel R.
Published: December 2016

Early treatment, soon after infection, reduces HIV transmissions and benefits patients. The Transmission Reduction Intervention Project (TRIP) evaluated a network intervention to detect individuals recently infected (in the past 6 months). TRIP was conducted in Greece (2013–2015) and focused on drug injector networks. Based on HIV status, testing history, and the results of an assay to detect recent infections, TRIP classified drug injector “Seeds” into groups: Recent Seeds (RS), and Control Seeds with Long-term HIV infection (LCS). The network members of RS and LCS were traced for two steps. The analysis included 23 RS, 171 network members of the RS, 19 LCS, and 65 network members of the LCS. The per-seed number of recents detected in the network of RS was 5 times the number in the network of LCS (Ratio RS vs. LCS: 5.23; 95% Confidence Interval (CI): 1.54–27.61). The proportion of recents among HIV positives in the network of RS (27%) was approximately 3 times (Ratio RS vs. LCS: 3.30; 95% CI: 1.04–10.43) that in the network of LCS (8%). Strategic network tracing that starts with recently infected persons could support public health efforts to find and treat people early in their HIV infection.
Comparative study between a spectral domain and a high-speed single-beam swept source OCTA system for identifying choroidal neovascularization in AMD
Authors: Told R. , Ginner L. , Hecht A. , Sacu S. , Leitgeb R. , Pollreisz A. , Schmidt-Erfurth U.
Published: December 2016

This comparative study between a SD- and SS-OCTA system for visualizing neovascular patterns in AMD, also assessed the influence of cataract on OCTA imaging. 25 eyes with active CNV (AMD) were documented by FA, ICGA and SD-OCT. Two OCTA devices were used: A custom built SS-OCTA (1050 nm, 400,000 A-scans/s, 5 × 5 mm, no image segmentation); AngioVue (OptoVue, CA, USA) SD-OCTA (840 nm, 70.000 A-scans/s, 3 × 3 mm, SSADA technology). Two retina experts graded CNV types and vascular patterns. Cataract influence on OCTA image quality was reported for the superficial retinal plexus (6 eyes). The SS-OCTA prototype showed more CNV lesions compared to the SD-OCTA system (p = 0.01). Overall sensitivity of SD- and SS-OCTA systems to detect CNV lesions was.32 and.68, respectively. The SS-OCTA system was able to detect discrete lesion characteristics better than the SD-OCTA. No significant difference was found in the ability to identify CNV in treatment-naïve eyes. There was no significant influence of cataract. The SS-OCTA prototype detected CNV-associated vascular patterns more reliably than the SD-OCTA system. This is attributed to the SS-OCTA system’s longer center wavelength and higher A-scan rate yielding higher definition and contrast of small neovascular structures. The SS-OCTA system used showed no advantage regarding cataract influence.
Vortex Laser based on III-V semiconductor metasurface: direct generation of coherent Laguerre-Gauss modes carrying controlled orbital angular momentum
Authors: Seghilani Mohamed S. , Myara Mikhael , Sellahi Mohamed , Legratiet Luc , Sagnes Isabelle , Beaudoin Grégoire , Lalanne Philippe , Garnache Arnaud
Published: December 2016

The generation of a coherent state, supporting a large photon number, with controlled orbital-angular-momentum L = ħl (of charge l per photon) presents both fundamental and technological challenges: we demonstrate a surface-emitting laser, based on III-V semiconductor technology with an integrated metasurface, generating vortex-like coherent state in the Laguerre-Gauss basis. We use a first order phase perturbation to lift orbital degeneracy of wavefunctions, by introducing a weak anisotropy called here “orbital birefringence”, based on a dielectric metasurface. The azimuthal symmetry breakdown and non-linear laser dynamics create “orbital gain dichroism” allowing selecting vortex handedness. This coherent photonic device was characterized and studied, experimentally and theoretically. It exhibits a low divergence (<1°) diffraction limited beam, emitting 49 mW output power in the near-IR at λ ≃ 1 μm, a charge l = ±1, … ±4 (>50 dB vortex purity), and single frequency operation in a stable low noise regime (0.1% rms). Such high performance laser opens the path to widespread new photonic applications.
ECAT1 is essential for human oocyte maturation and pre-implantation development of the resulting embryos
Authors: Liu Changyu , Li Min , Li Tianjie , Zhao Hongcui , Huang Jin , Wang Yun , Gao Qian , Yu Yang , Shi Qinghua
Published: December 2016

ECAT1 is a subunit of the subcortical maternal complex that is required for cell cycle progression during pre-implantation embryonic development; however, its exact function remains to be elucidated. Here we investigated the expression of ECAT1 in human ovarian tissue, oocytes and pre-implantation embryos and assessed its function by using RNA interference (RNAi) in oocytes. ECAT1 mRNA was highly expressed in human oocytes and zygotes, as well as in two-cell, four-cell and eight-cell embryos, but declined significantly in morulae and blastocysts. ECAT1 was expressed in the cytoplasm of oocytes and pre-implantation embryos and was localized more specifically in the cortical region than in the inner cytoplasm. RNAi experiments demonstrated that down-regulation of ECAT1 expression not only impaired spindle assembly and reduced maturation and fertilization rates of human oocytes but also decreased the cleavage rate of the resulting zygotes. In conclusion, our study indicates that ECAT1 may play a role in meiotic progression by maintaining the accuracy of spindle assembly in human oocytes, thus promoting oocyte maturation and subsequent development of the embryo.
Identification of heterotic loci associated with grain yield and its components using two CSSL test populations in maize
Authors: Wang Hongqiu , Zhang Xiangge , Yang Huili , Liu Xiaoyang , Li Huimin , Yuan Liang , Li Weihua , Fu Zhiyuan , Tang Jihua , Kang Dingming
Published: December 2016

Heterosis has widely been used to increase grain yield and quality. In this study, the genetic basis of heterosis on grain yield and its main components in maize were examined over 2 years in two locations in two test populations constructed from a set of 184 chromosome segment substitution lines (CSSLs) and two inbred lines (Zheng58 and Xun9058). Of the 169 heterotic loci (HL) associated with grain yield and its five components identified in CSSL × Zheng58 and CSSL × Xun9058 test populations, only 25 HL were detected in both populations. The comparison of quantitative trait loci (QTLs) detected in the CSSL population with HL detected in the two test populations revealed that only 15.46% and 17.35% of the HL in the given populations respectively, shared the same chromosomal regions as that of the corresponding QTLs and showed dominant effects as well as pleiotropism with additive and dominant effects. In addition, most of the HL (74.23% and 74.49%) had overdominant effects. These results suggest that overdominance is the main contributor to the effects of heterosis on grain yield and its components in maize, and different HL are associated with heterosis for different traits in different hybrids.
Astragaloside IV ameliorates allergic inflammation by inhibiting key initiating factors in the initial stage of sensitization
Authors: Bao Kai-fan , Yu Xi , Wei Xiao , Gui Li-li , Liu Hai-liang , Wang Xiao-yu , Tao Yu , Jiang Guo-rong , Hong Min
Published: December 2016

To illuminate the anti-allergy mechanism of astragaloside IV (AS-IV), we assessed its effects in a murine model of allergic contact dermatitis (ACD). AS-IV administered in the sensitization phase, rather than in the elicitation phase, dramatically alleviated the symptoms of allergic inflammation. We hypothesized that AS-IV exerts its anti-allergy effects by regulating the production of key pro-allergic cytokines based on the fact that interleukin (IL)-33 and thymic stromal lymphopoietin (TSLP) levels increase significantly in the initial stage of the sensitization phase. AS-IV administered in the initial stage of ACD inhibited TSLP and IL-33 expression and reduced the proportion of type-2 innate lymphoid cells (ILC2s). An in vitro study showed that the production of pro-allergic cytokines was significantly inhibited in AS-IV presenting HaCaT cells. We also verified that AS-IV administered only in the initial stage markedly alleviated inflammation, including ear swelling, Th2 cytokine expression, and histological changes. Taken together, these results suggest that AS-IV effectively ameliorates the progression of allergic inflammation by inhibiting key initiating factors, including TSLP and IL-33, and can be used to prevent and/or treat patients with ACD. Our data also suggest that these key pro-allergic cytokines are potential therapeutic targets for allergic diseases.
Incomplete and delayed Sox2 deletion defines residual ear neurosensory development and maintenance
Authors: Dvorakova Martina , Jahan Israt , Macova Iva , Chumak Tetyana , Bohuslavova Romana , Syka Josef , Fritzsch Bernd , Pavlinkova Gabriela
Published: December 2016

The role of Sox2 in neurosensory development is not yet fully understood. Using mice with conditional Islet1-cre mediated deletion of Sox2, we explored the function of Sox2 in neurosensory development in a model with limited cell type diversification, the inner ear. In Sox2 conditional mutants, neurons initially appear to form normally, whereas late- differentiating neurons of the cochlear apex never form. Variable numbers of hair cells differentiate in the utricle, saccule, and cochlear base but sensory epithelium formation is completely absent in the apex and all three cristae of the semicircular canal ampullae. Hair cells differentiate only in sensory epithelia known or proposed to have a lineage relationship of neurons and hair cells. All initially formed neurons lacking hair cell targets die by apoptosis days after they project toward non-existing epithelia. Therefore, late neuronal development depends directly on Sox2 for differentiation and on the survival of hair cells, possibly derived from common neurosensory precursors.
Biomarker MicroRNAs for Diagnosis, Prognosis and Treatment of Hepatocellular Carcinoma: A Functional Survey and Comparison
Authors: Shen Sijia , Lin Yuxin , Yuan Xuye , Shen Li , Chen Jiajia , Chen Luonan , Qin Lei , Shen Bairong
Published: December 2016

Hepatocellular Carcinoma (HCC) is one of the most common malignant tumors with high incidence and mortality rate. Precision and effective biomarkers are therefore urgently needed for the early diagnosis and prognostic estimation. MicroRNAs (miRNAs) are important regulators which play functions in various cellular processes and biological activities. Accumulating evidence indicated that the abnormal expression of miRNAs are closely associated with HCC initiation and progression. Recently, many biomarker miRNAs for HCC have been identified from blood or tissues samples, however, the universality and specificity on clinicopathological features of them are less investigated. In this review, we comprehensively surveyed and compared the diagnostic, prognostic, and therapeutic roles of HCC biomarker miRNAs in blood and tissues based on the cancer hallmarks, etiological factors as well as ethnic groups, which will be helpful to the understanding of the pathogenesis of biomarker miRNAs in HCC development and further provide accurate clinical decisions for HCC diagnosis and treatment.
Optical imaging of MMP-12 active form in inflammation and aneurysm
Authors: Razavian Mahmoud , Bordenave Thomas , Georgiadis Dimitris , Beau Fabrice , Zhang Jiasheng , Golestani Reza , Toczek Jakub , Jung Jae-Joon , Ye Yunpeng , Kim Hye-Yeong , Han Jinah , Dive Vincent , Devel Laurent , Sadeghi Mehran M.
Published: December 2016

Matrix metalloproteinase (MMP)-12 plays a key role in the development of aneurysm. Like other members of MMP family, MMP-12 is produced as a proenzyme, mainly by macrophages, and undergoes proteolytic activation to generate an active form. Accordingly, molecular imaging of the MMP-12 active form can inform of the pathogenic process in aneurysm. Here, we developed a novel family of fluorescent probes based on a selective MMP-12 inhibitor, RXP470.1 to target the active form of MMP-12. These probes were stable in complex media and retained the high affinity and selectivity of RXP470.1 for MMP-12. Amongst these, probe 3 containing a zwitterionic fluorophore, ZW800-1, combined a favorable affinity profile toward MMP-12 and faster blood clearance. In vivo binding of probe 3 was observed in murine models of sterile inflammation and carotid aneurysm. Binding specificity was demonstrated using a non-binding homolog. Co-immunostaining localized MMP-12 probe binding to MMP-12 positive areas and F4/80 positive macrophages in aneurysm. In conclusion, the active form of MMP-12 can be detected by optical imaging using RXP470.1-based probes. This is a valuable adjunct for pathophysiology research, drug development, and potentially clinical applications.
T cells mediate autoantibody-induced cutaneous inflammation and blistering in epidermolysis bullosa acquisita
Authors: Bieber Katja , Witte Mareike , Sun Shijie , Hundt Jennifer E. , Kalies Kathrin , Dräger Sören , Kasprick Anika , Twelkmeyer Trix , Manz Rudolf A. , König Peter , Köhl Jörg , Zillikens Detlef , Ludwig Ralf J.
Published: December 2016

T cells are key players in autoimmune diseases by supporting the production of autoantibodies. However, their contribution to the effector phase of antibody-mediated autoimmune dermatoses, i.e., tissue injury and inflammation of the skin, has not been investigated. In this paper, we demonstrate that T cells amplify the development of autoantibody-induced tissue injury in a prototypical, organ-specific autoimmune disease, namely epidermolysis bullosa acquisita (EBA) – characterized and caused by autoantibodies targeting type VII collagen. Specifically, we show that immune complex (IC)-induced inflammation depends on the presence of T cells – a process facilitated by T cell receptor (TCR)γδ and NKT cells. Because tissue damage in IC-induced inflammation is neutrophil-dependent, we further analyze the interplay between T cells and neutrophils in an experimental model of EBA. We demonstrate that T cells not only enhance neutrophil recruitment into the site of inflammation but also interact with neutrophils in lymphatic organs. Collectively, this study shows that T cells amplify the effector phase of antibody-induced tissue inflammation.
Glycoprotein 130 is associated with adverse postoperative clinical outcomes of patients with late-stage non-metastatic gastric cancer
Authors: Cao Yifan , Zhang Heng , Liu Hao , Lin Chao , Li Ruochen , Wu Songyang , He Hongyong , Li He , Xu Jiejie
Published: December 2016

The interaction of glycoprotein 130 (gp130) with the cytokines of Interleukin-6 (IL-6) family has proved to play a crucial part in several cancers. Our current study is designed to discover the clinical prognostic significance of gp130 in non-metastatic gastric cancer. We examined intratumoral gp130 expression in retrospectively enrolled 370 gastric cancer patients who underwent radical gastrectomy with standard D2 lymphadenectomy at Zhongshan Hospital of Fudan University during 2007 and 2008 by immunohistochemical staining. The expression of gp130 was significantly correlated with T classification, N classification and TNM stage (P = 0.003, P < 0.001 and P < 0.001, respectively; T, N, TNM refers to Tumor Invasion, Regional lymph node metastasis and Tumor Node Metastasis, respectively). Elevated intratumoral gp130 expression implied unfavourable overall survival (OS) (P < 0.001) and disease-free survival (DFS) (P < 0.001), respectively. Furthermore, among TNM II and III gp130-high patients, those who were treated with 5-fluorouracil (5-FU) based adjuvant chemotherapy had better OS (P < 0.001). The generated nomogram performed well in predicting the 3- and 5-year OS of gastric cancer patients. The incorporation of gp130 into contemporary TNM staging system would be of great significance to improve the current individual risk stratification. These findings contribute to better clinical management for those patients who would benefit from adjuvant chemotherapy.
Utility of real-time prospective motion correction (PROMO) on 3D T1-weighted imaging in automated brain structure measurements
Authors: Watanabe Keita , Kakeda Shingo , Igata Natsuki , Watanabe Rieko , Narimatsu Hidekuni , Nozaki Atsushi , Dan Rettmann  , Abe Osamu , Korogi Yukunori
Published: December 2016

PROspective MOtion correction (PROMO) can prevent motion artefacts. The aim of this study was to determine whether brain structure measurements of motion-corrected images with PROMO were reliable and equivalent to conventional images without motion artefacts. The following T1-weighted images were obtained in healthy subjects: (A) resting scans with and without PROMO and (B) two types of motion scans (“side-to-side” and “nodding” motions) with and without PROMO. The total gray matter volumes and cortical thicknesses were significantly decreased in motion scans without PROMO as compared to the resting scans without PROMO (p < 0.05). Conversely, Bland–Altman analysis indicated no bias between motion scans with PROMO, which have good image quality, and resting scans without PROMO. In addition, there was no bias between resting scans with and without PROMO. The use of PROMO facilitated more reliable brain structure measurements in subjects moving during data acquisition.
Xylanase Treatment Suppresses Light- and Heat-Induced Yellowing of Pulp
Authors: Zhang Daolei , Li Xuezhi , Wang Meimei , Ye Yanxin , Du Jian , Lu Xianqin , Zhao Jian
Published: December 2016

Xylanase is commonly applied in pulp and paper industries to ease cost-related and environmental pressures. The effect of xylanase treatment on pulp bleaching is well-established, however, few studies were conducted on the effects of xylanase treatment in pulp yellowing, especially the mechanism of pulp yellowing inhibition by xylanase treatment. In this study, pure xylanase (EC 3.2.1.8) was applied to treat wheat straw chemical pulp (CP) and poplar chemi-thermo-mechanical pulp (CTMP) to determine their effects on pulp brightness and on light- and heat-induced yellowing. The xylanase treatment decreased the post-color number of the pulps during light- and heat-induced yellowing. However, differences were observed in the yellowing inhibition between the wheat straw CP and poplar CTMP. The changes in chemical components of pulps after the xylanase treatment, for example, lignin, hemicellulose, and HexA contents, and analysis of UV–vis absorption spectra and Fourier transform infrared-attenuated total reflectance spectrum were used to explore the pulp yellowing inhibition causes by the xylanase treatment.
Metagenomic profiling of historic Colorado Front Range flood impact on distribution of riverine antibiotic resistance genes
Authors: Garner Emily , Wallace Joshua S. , Argoty Gustavo Arango , Wilkinson Caitlin , Fahrenfeld Nicole , Heath Lenwood S. , Zhang Liqing , Arabi Mazdak , Aga Diana S. , Pruden Amy
Published: December 2016

Record-breaking floods in September 2013 caused massive damage to homes and infrastructure across the Colorado Front Range and heavily impacted the Cache La Poudre River watershed. Given the unique nature of this watershed as a test-bed for tracking environmental pathways of antibiotic resistance gene (ARG) dissemination, we sought to determine the impact of extreme flooding on ARG reservoirs in river water and sediment. We utilized high-throughput DNA sequencing to obtain metagenomic profiles of ARGs before and after flooding, and investigated 23 antibiotics and 14 metals as putative selective agents during post-flood recovery. With 277 ARG subtypes identified across samples, total bulk water ARGs decreased following the flood but recovered to near pre-flood abundances by ten months post-flood at both a pristine site and at a site historically heavily influenced by wastewater treatment plants and animal feeding operations. Network analysis of de novo assembled sequencing reads into 52,556 scaffolds identified ARGs likely located on mobile genetic elements, with up to 11 ARGs per plasmid-associated scaffold. Bulk water bacterial phylogeny correlated with ARG profiles while sediment phylogeny varied along the river’s anthropogenic gradient. This rare flood afforded the opportunity to gain deeper insight into factors influencing the spread of ARGs in watersheds.
Arbitrary quantum state engineering in three-state systems via Counterdiabatic driving
Authors: Chen Ye-Hong , Wu Qi-Cheng , Huang Bi-Hua , Song Jie , Xia Yan
Published: December 2016

A scheme for arbitrary quantum state engineering (QSE) in three-state systems is proposed. Firstly, starting from a set of complete orthogonal time-dependent basis with undetermined coefficients, a time-dependent Hamiltonian is derived via Counterdiabatic driving for the purpose of guiding the system to attain an arbitrary target state at a predefined time. Then, on request of the assumed target states, two single-mode driving protocols and a multi-mode driving protocol are proposed as examples to discuss the validity of the QSE scheme. The result of comparison between single-mode driving and multi-mode driving shows that multi-mode driving seems to have a wider rang of application prospect because it can drive the system to an arbitrary target state from an arbitrary initial state also at a predefined time even without the use of microwave fields for the transition between the two ground states. Moreover, for the purpose of discussion in the scheme’s feasibility in practice, a polynomial ansatz as the simplest exampleis used to fix the pulses. The result shows that the pulses designed to implement the protocols are not hard to be realized in practice. At the end, QSE in higher-dimensional systems is also discussed in brief as a generalization example of the scheme.