bluuebunny/crossref_metadata_2025 huggingface dataset:
Architecture and Recreation as a Political Tool—Seaside Architectural Heritage of the Worker Holiday Fund (WHF) in the Era of the Polish People’s Republic (1949–1989)
Authors: Bal Wojciech , Czałczyńska-Podolska Magdalena
Published: December 2021

The Worker Holiday Fund (WHF) was set up just after the Second World War as a state-dependent organization that arranged recreation for Polish workers under the socialist doctrine. The communist authorities turned organized recreation into a tool of indoctrination and propaganda. This research aims to characterize the seaside tourism architecture in the Polish People’s Republic (1949–1989) against the background of nationalized and organized tourism being used as a political tool, to typify the architecture and to verify the influence of politics on the development of holiday architecture in Poland. The research methodology is based on historical and interpretative studies (iconology, iconography and historiography) and field studies. The research helped distinguish four basic groups of holiday facilities: one form of adapted facilities (former villas and boarding houses) and three forms of new facilities (sanatorium-type, pavilion-type and lightweight temporary facilities, such as bungalows and cabins). The study found that each type of holiday facility was characterized by certain political significance and social impact. Gradual destruction was the fate of a significant part of WHF facilities, which, in the public awareness, are commonly associated with the past era of the Polish People’s Republic (PRL) as an “unwanted heritage”.
Understanding the Social Licence of Carbon Farming in the Australian Rangelands
Authors: Baumber Alex , Cross Rebecca , Waters Cathy , Metternicht Graciela , Kam Hermann
Published: December 2021

Carbon farming has expanded in Australia’s rangelands over recent years, incentivised under the Australian Government’s Emissions Reduction Fund. While this has largely been driven by economic benefits for landholders, the long-term viability of the carbon farming industry depends on its ability to obtain and maintain a social licence to operate in affected communities. Using a combination of survey, interview and focus group methods, involving key stakeholders in far-western New South Wales (NSW), this study reveals that the greatest threat to the social licence of carbon farming is the lack of confidence in governance related to policy complexity and uncertainty. Procedural fairness is a relative strength because of the involvement of trusted community members, and the trust-building strategies employed by the aggregators who recruit landholders to carbon farming. Perceptions of distributional fairness are strengthened by the benefits beginning to flow through rangeland communities, but are weakened by concerns around the equity of eligibility and the land management rules. A focus on participatory policy development, aligning rules with local values and local-scale trust building, is required in order to enhance the social licence for carbon farming in the NSW rangelands.
Measurements of Intercultural Teamwork Competence and Its Impact on Design Students’ Competitive Advantages
Authors: Zhang Xiu-Yue , Zhu Xu-Guang , Tu Jui-Che , Yi Minzhe
Published: December 2021

Main issue: This article aims to measure intercultural teamwork competence and assess its impact on career competitive advantages for design students in order to determine how effective and competitive team members are in culturally diverse settings. Sampling: there were 51 participants (48 effective) in pretesting and 405 (338 effective) in formal testing. Participants were students from three colleges of design in Zhejiang Province of China. Statistical tool: this study used the on-line platform of wenjuanxing (wjx.cn) for data collection and SPSS software for data analysis. Methods: data were collected through on-line questionnaires, and then processed through factor analysis, t-test, and stepwise regression. Results: (1) TWC-CQ scale was formed to measure intercultural teamwork competence; (2) there were no statistically significant differences among participating design students (junior and senior) in intercultural teamwork competencies; (3) key competitive advantage = 0.347 × T-behavioral CQ + 0.232 × T-metacognitive CQ + 0.172 × T-motivational CQ + 0.124 × T-cognitive CQ. Conclusions: intercultural teamwork competence and its positive impact on design students’ competitive advantages could be measured. Implications: theoretical and practical implications were summed up for future studies.
Competencies for Agricultural Advisors in Innovation Support
Authors: Lybaert Charlotte , Debruyne Lies , Kyndt Eva , Marchand Fleur
Published: December 2021

The expectation that agricultural advisors will facilitate Interactive Innovation is accompanied by novel expectations for their competency profile. In addition to their traditional technical basis, advisors are now expected to organise multi-actor processes, facilitate learning, mediate conflict, etc. Innovation support services are inherently diverse. To date, no precise list of competencies required by agricultural advisors to support Interactive Innovation has been defined. To form the basis for a competency profile, we examine the competencies currently being expected from an agricultural advisor. This suggested profile, developed in the context of the H2020 i2connect project, is based on a literature review, semi-structured interviews with co-creation experts, and an online validation workshop. We explore five themes: (a) basic disposition and attitude, (b) content competence, (c) methodological competence, (d) organisational competence, and (e) reflection, learning, and personal development. In practice, the profile can be used as either a tool for setting up co-creation processes or as the foundation for the development of new training materials. We conclude with a recommendation to create teams of advisors rather than relying on individuals, as a team is more likely to comprise the diversity of required competencies.
Vibrational Communication of Scolypopa australis (Walker, 1851) (Hemiptera: Ricaniidae)—Towards a Novel Sustainable Pest Management Tool
Authors: Sullivan Nicola Jayne , Avosani Sabina , Butler Ruth C. , Stringer Lloyd D.
Published: December 2021

A study was undertaken to determine whether Scolypopa australis, the passionvine hopper, communicates using substrate-borne vibrations, as its use of such signals for communication is currently unknown. This insect is a costly pest to the kiwifruit industry in New Zealand, where few pest management tools can be used during the growing season. Vibrations emitted by virgin females and males of S. australis released alone on leaves of Griselinia littoralis were recorded with a laser vibrometer to identify and characterise potential spontaneous calling signals produced by either sex. In addition to single-insect trials, preliminary tests were conducted with female–male pair trials to determine whether individuals exchanged signals. The signal repertoire of S. australis includes a male calling signal and two female calling signals. However, no evidence of duetting behaviour that is potentially necessary for pair formation has been found to date. Our outcome suggests that a deeper understanding of the role of vibrational communication employed by S. australis is needed, and by disclosing the pair formation process, a new residue-free pest management tool against this pest may be developed. In addition, this vibration-based tool could contribute to future biosecurity preparedness and response initiatives.
Influencing Factors of Azerbaijan and China’s Sustainable Tourism Development Strategy under the One Belt One Road Initiative
Authors: Liu Yiming , Suk Sunhee
Published: December 2021

After the Belt and Road Initiative (BRI) was put forward, the tourism industry between Azerbaijan and China developed rapidly, and gradually became an important area of cooperation between the two countries to promote economic cooperation, non-governmental exchange, and cultural exchange. However, there is currently a lack of research on the development strategy of tourism cooperation between the two countries under the BRI. This paper aims to explore the development strategy of sustainable tourism cooperation between Azerbaijan and China under BRI through a hybrid model of SWOT (Strengths, Weaknesses, Opportunities, and Threats) and AHP (Analytic Hierarchy Process). The factor elements of the SWOT matrix introduce ambiguity due to the complex layering of elemental importance. Therefore, the AHP method was used to systematically evaluate the importance of strategic factors and reduce the subjective bias caused by the SWOT analysis method. By constructing the Delphi-AHP-SWOT strategic quadrilateral and calculating the azimuth of the strategy type, the type and intensity map of the tourism cooperation development strategy between Azerbaijan and China under the BRI is drawn. Finally, it is concluded that the development strategy of tourism cooperation between Azerbaijan and China is a pioneering development strategy. Suggestions are given for future development. This paper provides a valuable experience for the sustainable development of tourism cooperation among countries under the BRI.
Impact of Environmental Pollution in the Sustainability of Architectural Heritage: Case Study from Cartagena of India, Colombia
Authors: Cano Heidis , Ríos-Rojas John Fredy , Hernández-Fernández Joaquin , Bernal Herrera Wilson , Bautista Betancur Mayka , De La Hoz Vélez Lorcy , Agámez González Lidy
Published: December 2021

Limited studies have been carried out in emerging nations on the correlation among the environmental pollution, economic factors, and architectural heritage. For this reason, this research presents an assessment of environmental parameter values on materials deterioration used in architectural heritage in Cartagena de Indias; furthermore, it depicts the effect of heritage degradation on socioeconomic aspects of people whose livelihood depends on trade, tourism, and service activities. Dose-response functions were used for estimating of deterioration of carbon steel, copper, and zinc caused by relative humidity (RH), temperature (T), sulphur dioxide deposition (DSO2), and chloride deposition (DCl−). In addition, socioeconomic impact on architectural heritage was studied using a Socioeconomic Impact Survey (SEIS), with the sample of 174 individuals who work in areas of great architectural value in the city. The results show a corrosion rate (Vcorr) in the range of 80 < Vcorr < 200, 2.8 < Vcorr < 5.6 and 4.2 < Vcorr < 8.4 μm/year for carbon steel, copper, and zinc, respectively, due to the high level of pollutants. The high deterioration jointly with the lack of citizen culture affect the architectural heritage monuments causing a negative impact in several economic aspects. The establishment of public programs is essential for the conservation of the heritage monuments of the city.
Commoning Adaptive Heritage Reuse as a Driver of Social Innovation: Naples and the Scugnizzo Liberato Case Study
Authors: Fava Federica
Published: December 2021

The adaptive reuse of cultural heritage assets is often problematic. What emerges is the urgency of a thoughtful negotiation between built forms and emerging needs and requests. In this view, a fruitful trajectory of development arises in commoning heritage by means of adaptive reuse. Hence, the purpose of this article is to investigate how community-led adaptive heritage re-use practices contribute to social innovation in terms of new successful model of urban governance, by providing a specific focus on innovative aspects that emerge in both heritage and planning sectors. Therefore, it also aims to improve the knowledge in the innovative power of heritage when conceptualized as performative practice. To this end, the paper presents the adaptation process of a former church complex located in Naples, today Scugnizzo Liberato, one of the bottom-up initiatives recognized by the Municipality of Naples as part of the urban commons network of the city. The research results are based on desk research, a literature review, and interviews with experts and activists, conducted as part of the OpenHeritage project (Horizon 2020). Initial evidence shows that profound citizen involvement throughout the whole heritage-making process might generate innovative perspectives in urban governance as well as conservation planning practice.
Optimization Model of Transit Route Fleet Size Considering Multi Vehicle Type
Authors: Liu Huasheng , Zhao Yuqi , Li Jin , Li Yu , Gao Xiangtao
Published: December 2021

This paper proposes a bus line capacity optimization design model considering the scale of multiple vehicles, which is achieved by minimizing system operating costs and user costs. The proposed model takes into account the difference of passenger demand in different periods, and can get the optimal headway and delivery and reserve plan. In order to prove that the method can effectively minimize the cost, we solved a numerical example and compared the cost of the method in multi-transit model planning. Furthermore, the optimization results show that the total costs (TC) were reduced by 14.48%. Among them, the user costs (UC) decreased by 30.38% and the operator costs (OC) increased by 4.18%. Sensitivity analyses are presented to verify the validity of the model. The analysis results show that multi size bus optimization can reduce the total cost, especially the user cost in a certain cost weight interval. Besides this, the cost weight which reflects the passenger volume and waiting time value, optional bus size and cross-section passenger volume all affect vehicle scheme and system cost.
Linking FDI and Sustainable Environment in EU Countries
Authors: Dornean Adina , Chiriac Irina , Rusu Valentina Diana
Published: December 2021

The aim of this study is to emphasize the link between the foreign direct investments (FDIs) and the sustainable environment in EU countries. We also focus on investigating the influence of other factors related to business environment on FDIs, considering the investors’ sustainable choice for the host countries, grouped according to FTSE Russell criteria. Using panel methodology and applying Ordinary Least Squares (OLS) method of data analysis, the authors reached the conclusion that a better-rated business environment, with concern for sustainability, has more of a chance to attract larger sums of FDIs, mostly in the case of developed economies. This fact proves that the main advantage considered by a foreign investor in developed EU countries is represented by CO2 emissions (sustainable environment) and a good ease of doing business environment. The study highlights the factors that influence the decision of investing in developed countries, rather than in emerging and frontier ones. This paper contributes to the existing literature by identifying the group of countries which need a more sustainable approach to attract a large amount of FDIs, given that the inflow of FDIs is a crucial factor of economic growth, a possible source of innovation and technology, and a way to reduce poverty.
A Methodological Framework for Life Cycle Sustainability Assessment of Construction Projects Incorporating TBL and Decoupling Principles
Authors: Srivastava Shivam , Raniga Usha Iyer , Misra Sudhir
Published: December 2021

The triple bottom line (TBL) principle encompasses the idea of continued economic and social well-being with minimal or reduced environmental pressure. However, in construction projects, the integration of social, economic, and environmental dimensions from the TBL perspective remains challenging. Green building rating tools/schemes, such as Green Rating for Integrated Habitat Assessment (GRIHA), Leadership in Energy and Environment Design (LEED), Building Research Establishment Environment Assessment (BREEAM), and their criteria, which serve as a yardstick in ensuring sustainability based practices and outcomes, are also left wanting. These green building rating tools/schemes not only fail to comprehensively evaluate the three dimensions (social, economic, and environment) and interaction therewith, but also lack in capturing a life cycle approach towards sustainability. Therefore, this study intends to address the aforementioned challenges. The first part of this study presents the concept of sustainable construction as a system of well-being decoupling and impact decoupling. Findings in the first part of this study provide a rationale for developing a methodological framework that not only encapsulates a TBL based life cycle approach to sustainability assessment in construction, but also evaluates interactions among social and economic well-being, and environmental pressure. In methodological framework development, two decoupling indices were developed, namely, the phase well-being decoupling index (PWBDIK) and phase impact decoupling index (PIDIK). PWBDIK and PIDIK support the evaluation of interdependence among social and economic well-being, and the environmental pressure associated with construction projects in different life cycle phases. The calculation underpinning the proposed framework was illustrated using three hypothetical cases by adopting criteria from GRIHA Precertification and GRIHA v.2019 schemes. The results of these cases depict how the interactions among different dimensions (social, economic, and environment) vary as they move from one phase to another phase in a life cycle. The methodological framework developed in this study can be tailored to suit the sustainability assessment requirements for different phases and typologies of construction in the future.
Sustainable High-Tech Brick Production with Energy-Oriented Consumption: An Integrated Possibilistic Approach Based on Criteria Interdependencies
Authors: Solgi Ehsan , Gitinavard Hossein , Tavakkoli-Moghaddam Reza
Published: December 2021

Brick making contributes significantly to the of supply materials for the building industry. The majority of brick production sectors, especially in developing countries, employ polluting and energy-inefficient technologies. Due to the increasing pressures on manufacturing firms to improve economic performance and growing environmental protection issues, sustainable and clean production is the main concern for brick makers. This paper considers the technological, economic, environmental, social, and energy-oriented criteria to select the optimal brick production technologies. Therefore, technology selection is viewed as a multi-criteria group decision-making (MCGDM) problem. This research proposes a novel hybrid fuzzy MCGDM (HFMCGDM) model to tackle the problem. In this respect, first of all, the modified triangular fuzzy pair-wise comparison (MTFPC) method is proposed to compute the local weights of criteria and sub-criteria. Then, a fuzzy DEMATEL (FDEMATEL) method is presented to calculate the interdependencies between and within the criteria. Moreover, the integration of MTFPC and FDEMATEL methods is applied to calculate the global criteria weights. Afterward, a novel method is proposed to determine the experts’ weight. Considering the last aggregation approach to diminish data loss, a new version of a fuzzy TOPSIS method is proposed to find the local and global priorities of the candidates. Then, a case study is given to demonstrate the applicability and superiority of the proposed methodology. To get a deeper view about considering kilns, energy and environmental performance of which has been investigated. Moreover, a comparative analysis is presented to illuminate the merits of the proposed methodology. Eventually, a sensitivity analysis is conducted to peruse the influence of criteria weights on ranking order.
Are ESG Shares a Safe Haven during COVID-19? Evidence from the Arab Region
Authors: Mousa Musaab , Saleem Adil , Sági Judit
Published: December 2021

The world experienced significant changes in its social and economic lives in 2020–21. Major stock markets experienced an immediate decline. This paper attempts to examine the impact of COVID-19 on stock market performance as well as to identify the differences between the responses of ESG stocks and normal stocks to pandemic conditions in the Arab region. Daily time series for three years between March 2019 and March 2021 were collected for the S&P Pan Arab Composite index and S&P/Hawkamah ESG Pan Arab Index. We used a generalized autoregressive conditional heteroscedasticity (GARCH) model to measure market shocks and a non-linear autoregressive distributed lagged (NARDL) regression model to display the relationship between COVID-19 measurements and the performance of stock indexes. The findings suggest that the volatilities of ESG portfolios and conventional ones were equally affected in the pre-COVID period. However, in the post-COVID period, the magnitude of volatility in the ESG stock index was significantly less compared to that of the conventional stock index. The results also revealed that in the ESG market, shock tended to remain for a shorter period. Furthermore, the ESG index was not affected by the number of confirmed cases and deaths. However, evidence of asymmetric long-run cointegration existed between the S&P index and number of cases and deaths. Increases in the numbers of cases and deaths caused a decline in market index, whereas the reverse trends were observed in the retreat of the pandemic.
Exploring the Moderating Role of Social and Ethical Practices in the Relationship between Environmental Disclosure and Financial Performance: Evidence from ESG Companies
Authors: Chouaibi Salim , Rossi Matteo , Siggia Dario , Chouaibi Jamel
Published: December 2021

Environmental disclosure is the latest novelty in the corporate reporting field. In fact, it is a tool that can better represent the capacity of companies in creating financial performance over time. Therefore, this paper analyzes whether environmental disclosure (ED) practiced by firms listed on the ESG index affects their financial performance (FP) using the moderating effect of social and ethical practices. The analysis includes a linear regression using panel data from Thomson Reuters and Bloomberg databases. Panel data were collected from a sample of 523 companies listed on the North American and West European stock exchanges. The obtained results show a positive and significant relationship between environmental disclosure (ED) and financial performance (FP). This implies that a strong environmental disclosure increases financial performance while a weak one decreases it. Furthermore, the study suggests a moderating effect of social and ethical practices in the link between environmental disclosure and the firm’s financial performance. In fact, these findings provide interesting insights for academic practitioners and regulators who are interested in discovering environmental disclosure, firm’s performance, and social and ethical practices. These findings also provide insights to stakeholders and regulators on the crucial need to integrate more social and environmental regulations to promote sustainability. Moreover, this paper fills the gaps existing in previous studies that ignore the moderating role of social and ethical practices in the relationship between environmental disclosure and financial performance.
Analysis of Consumer Preference for Green Tea with Eco-Friendly Certification in China
Authors: Nam Kyungsoo , Qiao Yiyang , Ahn Byeong-il
Published: December 2021

The eco-friendly certification system is designed to ensure safe agricultural products to consumers while minimizing environmental pollution. However, despite its advantages, it is not widely adopted due to a possible decrease of farmers’ income. In order to provide implication for activating the eco-friendly certification system, this paper examines the attributes of green tea which affect consumers’ preferences and estimates consumers’ willingness to pay (WTP) for the eco-friendly certification in China. A choice experiment survey is employed for data collection, and the random utility model is used to estimate the preference for the certification and quality of green tea. The attribute that yields the highest marginal WTP turns out to be the organic certification for which WTP is $115.9/250 g higher than for no certification. Also, the analytical results indicate that the group with high trust is willing to pay up to $214.6/250 g more for green tea with organic certification compared to the one with no certification. The empirical results suggest that it is important to build the consumers’ awareness and trust toward the certification to activate the eco-friendly certification system.
Transfer Capital or Transfer Deficit: A Dual Perspective of English Learning of ESL College Transfer Students
Authors: Foung Dennis , Cheung Kin
Published: December 2021

This research addressed a research gap in scrutinizing the language problems of English as a second language (ESL) transfer students (TSs) with regard to the aspects of “transfer deficit” and “transfer capital”, instead of simply labelling the use of English as a “transfer deficit”. One hundred and twenty-four TSs participated in this qualitative study. From qualitative content analysis, three main categories were identified: (a) English competence as transfer capital; (b) English competence as transfer deficit; and (c) transition from deficit to capital. Based on the results, educational practitioners are advised to pay attention to the specific implications of proficiency-based courses, with support measures not limited to essay-writing or referencing skills, but including advanced research writing genres such as the Capstone Project.
The Analysis of the Factors Influencing the Severity of Bicyclist Injury in Bicyclist-Vehicle Crashes
Authors: Macioszek Elżbieta , Granà Anna
Published: December 2021

Transportation and technological development have for centuries strongly influenced the shaping of urbanized areas. On one hand, it undoubtedly brings many benefits to their residents. However, also has a negative impact on urban areas and their surroundings. Many transportation and technological solutions lead, for example, to increased levels of pollution, noise, excessive energy use, as well as to traffic accidents in cities. So, it is important to safe urban development and sustainability in all city aspects as well as in the area of road transport safety. Due to the long-term policy of sustainable transport development, cycling is promoted, which contributes to the increase in the number of this group of users of the transport network in road traffic for short-distance transport. On the one hand, cycling has a positive effect on bicyclists’ health and environmental conditions, however, a big problem is an increase in the number of serious injuries and fatalities among bicyclists involved in road incidents with motor vehicles. This study aims to identify factors that influence the occurrence and severity of bicyclist injury in bicyclist-vehicle crashes. It has been observed that the factors increasing the risk of serious injuries and deaths of bicyclists are: vehicle driver gender and age, driving under the influence of alcohol, exceeding the speed limit by the vehicle driver, bicyclist age, cycling under the influence of alcohol, speed of the bicyclist before the incident, vehicle type (truck), incident place (road), time of the day, incident type. The obtained results can be used for activities aimed at improving the bicyclists’ safety level in road traffic in the area of analysis.
The Effectiveness of Online Education in Basic Medical Sciences Courses during the COVID-19 Pandemic in Saudi Arabia: Cross-Sectional Study
Authors: Alblihed Mohamed A. , Aly Syed Moyn , Albrakati Ashraf , Eldehn Ahmed Fathy , Ali Shaimaa Ahmed Awad , Al-Hazani Tahani , Albarakati Mohammed Hamed , Abdel Daim Mohamed , Al-sharif Anas , Albarakati Alaa Jameel A. , Elmahallawy Ehab Kotb
Published: December 2021

Coronavirus-19 (COVID-19) is an emerging pandemic that has caused significant disruptions to education, resulting in marked scholar closures across the world. The present study documents the student perception of the online educational process at six universities in Saudi Arabia during the COVID-19 lockdown period. The individual variable factors, i.e., institution, sex, and blended learning, that might influence student perception were also investigated. In this respect, a questionnaire that assessed the impact of COVID-19 on the online learning process and student perception was emailed to a total of 17,230 students from 3 institutions (medical, applied health and dental students) affiliated with six universities in Saudi Arabia. Principal component analysis (PCA) was performed to determine the validity of the questionnaire. A total of 4850 (28%) students responded to the questionnaire and 46.9% (n = 2275) of the respondents were highly satisfied with their online education. Analyses of the scores from the students showed a highly significant difference (p = 0.000) between the male and female students. Applied health students (highly satisfied score = 63.13) were the most satisfied with their online education, while medical students (highly satisfied score = 39.3) were the least satisfied with this education. In addition, applied health, dental and medical students showed a significantly higher score in favor of blended learning after schools re-open (p = 0.000). Collectively, student satisfaction with online education was common, and blended learning was preferred over the traditional format by both genders and all the students. The present data provide interesting contributions and evidence for policy makers and implementers, which might be helpful for the judgment side of decision making.
Topic Extraction and Interactive Knowledge Graphs for Learning Resources
Authors: Badawy Ahmed , Fisteus Jesus A. , Mahmoud Tarek M. , Abd El-Hafeez Tarek
Published: December 2021

Humanity development through education is an important method of sustainable development. This guarantees community development at present time without any negative effects in the future and also provides prosperity for future generations. E-learning is a natural development of the educational tools in this era and current circumstances. Thanks to the rapid development of computer sciences and telecommunication technologies, this has evolved impressively. In spite of facilitating the educational process, this development has also provided a massive amount of learning resources, which makes the task of searching and extracting useful learning resources difficult. Therefore, new tools need to be advanced to facilitate this development. In this paper we present a new algorithm that has the ability to extract the main topics from textual learning resources, link related resources and generate interactive dynamic knowledge graphs. This algorithm accurately and efficiently accomplishes those tasks no matter how big or small the texts are. We used Wikipedia Miner, TextRank, and Gensim within our algorithm. Our algorithm’s accuracy was evaluated against Gensim, largely improving its accuracy. This could be a step towards strengthening self-learning and supporting the sustainable development of communities, and more broadly of humanity, across different generations.
Efficient Extraction of the RuBisCO Enzyme from Spinach Leaves Using Aqueous Solutions of Biocompatible Ionic Liquids
Authors: Valente Ana I. , Ferreira Ana M. , Almeida Mafalda R. , Mohamadou Aminou , Freire Mara G. , Tavares Ana P. M.
Published: December 2021

Ribulose-1,5-biphosphate carboxylase/oxygenase (RuBisCO) is the most abundant protein on the planet, being present in plants, algae and various species of bacteria, with application in the pharmaceutical, chemical, cosmetic and food industries. However, current extraction methods of RuBisCO do not allow high yields of extraction. Therefore, the development of an efficient and selective RuBisCOs’ extraction method is required. In this work, aqueous solutions of biocompatible ionic liquids (ILs), i.e., ILs derived from choline and analogues of glycine-betaine, were applied in the RuBisCO’s extraction from spinach leaves. Three commercial imidazolium-based ILs were also investigated for comparison purposes. To optimize RuBisCO’s extraction conditions, response surface methodology was applied. Under optimum extraction conditions, extraction yields of 10.92 and 10.57 mg of RuBisCO/g of biomass were obtained with the ILs cholinium acetate ([Ch][Ac]) and cholinium chloride ([Ch]Cl), respectively. Circular dichroism (CD) spectroscopy results show that the secondary structure of RuBisCO is better preserved in the IL solutions when compared to the commonly used extraction solvent. The obtained results indicate that cholinium-based ILs are a promising and viable alternative for the extraction of RuBisCO from vegetable biomass.
On the Interpretation of the Balance Function
Authors: Vechernin Vladimir
Published: December 2021

We construct a simple toy model and explicitly demonstrate that the balance function (BF) can become negative for some values of the rapidity separation and hence cannot have any probabilistic interpretation. In particular, the BF cannot be interpreted as the probability density for the balancing charges to occur separated by the given rapidity interval.
Modulation of Auditory Perception Laterality under Anxiety and Depression Conditions
Authors: Estalayo-Gutiérrez Beatriz , Álvarez-Pasquín María José , Germain Francisco
Published: December 2021

The objective of this work is to confirm the asymmetry in non-linguistic auditory perception, as well as the influence of anxiety-depressive disorders on it. Eighty-six people were recruited in the emotional well-being group, fifty-six in the anxiety group, fourteen in the depression group, and seventy-seven in the mixed group. In each group, audiograms were obtained from both ears and the differences were statistically analyzed. Differences in hearing sensitivity were found between both ears in the general population, such differences increased in people with anxiety-depressive disorders. When faced with anxiety-depressive disorders, the right ear suffered greater hearing loss than the left, showing peaks of hyper-hearing at the frequency of 4000 Hz in the anxiety subgroup, and hearing loss in the depression subgroup. In relation to anxiety, the appearance of the 4:8 pattern was observed in the right ear when the person had suffered acute stress in the 2 days prior to the audiometry, and in both ears if they had suffered stress in the 3–30 days before said stress. In conclusion, the advantage of the left ear in auditory perception was increased with these disorders, showing a hyperaudition peak in anxiety and a hearing loss in depression.
Sub-Hz Differential Rotational Spectroscopy of Enantiomers
Authors: Satterthwaite Lincoln , Koumarianou Greta , Sorensen Daniel , Patterson David
Published: December 2021

We demonstrate for the first time high-precision differential microwave spectroscopy, achieving sub-Hz precision by coupling a cryogenic buffer gas cell with a tunable microwave Fabry–Perot cavity. We report statistically limited sub-Hz precision of (0.08 ± 0.72) Hz, observed between enantiopure samples of (R)-1,2-propanediol and (S)-1,2-propanediol at frequencies near 15 GHz. We confirm highly repeatable spectroscopic measurements compared to traditional pulsed-jet methods, opening up new capabilities in probing subtle molecular structural effects at the 10−10 level and providing a platform for exploring sources of systematic error in parity-violation searches. We discuss dominant systematic effects at this level and propose possible extensions of the technique for higher precision.
Anatomical Injection Guidelines for Glabellar Frown Lines Based on Ultrasonographic Evaluation
Authors: Kim Soo-Bin , Kim Hyoung-Moon , Ahn Haeryun , Choi You-Jin , Hu Kyung-Seok , Oh Wook , Kim Hee-Jin
Published: December 2021

When botulinum neurotoxin (BoNT) is injected to treat glabellar frown lines, the corrugator supercilia muscle (CSM) and procerus muscles are the main targets. Although there have been many studies on the treatment of glabellar frown lines, no study has confirmed the dynamic movement under ultrasonography (US). This study examined and evaluated dynamic muscle movements under US, thereby providing more effective BoNT injection guidelines for glabellar frowning. Glabellar frowning was categorized as either Type A or B. Type A is the general frowning pattern in which vertical wrinkles are made by contracting the CSM and procerus muscles (81%, n = 13). On US images, the procerus muscle thickens and the bilateral CSMs contract. Type B is an upward frowning pattern demonstrating upward elevation of vertical wrinkles due to hyperactive contraction of the frontalis muscle during frowning (19%, n = 3). On US images, the hypoechoic frontalis muscle thickens, forming horizontal forehead lines. After BoNT injection into the CSM and frontalis muscle but not the procerus muscle, Type B patterns showed improvements in the vertical crease and horizontal forehead line. Both types showed improvement in glabellar frown lines after conventional injection, but the horizontal forehead line did not improve in Type B. Type B wrinkles improved after additional injections into the frontalis muscle. This study provided novel anatomical findings related to the injection of glabellar frown lines with BoNT. Preliminary analysis and optimized procedures using US will enable more effective and safer injections.
The Impact of a Cervical Collar on Intracranial Pressure in Traumatic Brain Injury Patients: A Systematic Review and Meta-Analysis
Authors: Bazaie Naif , Alghamdi Ibrahim , Alqurashi Naif , Ahmed Zubair
Published: December 2021

Purpose: Although the use of a cervical collar in the prehospital setting is recommended to prevent secondary spinal cord injuries and ensure spinal immobilization, it is not known what effects this has on raising intracranial pressure (ICP) in traumatic brain injury (TBI) patients. In the absence of studies measuring ICP in the prehospital setting, the aim of this study was to systematically review the data related to ICP changes measured after presentation at the hospital in patients who had arrived wearing cervical collars. Methods: We searched Medline (PubMed), Embase, CINAHL, and Google Scholar for studies that investigated in-hospital ICP changes in TBI patients arriving at the hospital wearing collars. Titles, abstracts, and full texts were then searched for inclusion in the study. A narrative synthesis, as well as a meta-analysis, was performed. Results: Of the 1006 studies identified, only three met the inclusion/exclusion criteria. The quality of the three included studies was moderate and the risk of bias was low. All three studies used the Laerdal Stifneck collar, but all studies showed an increase in ICP after application of the collar. A further three studies that measured ICP but did not fit the systematic search were also included due to low patient numbers. A meta-analysis of the pooled data confirmed a significant increase in ICP, although between the four studies, only 77 patients were included. The meta-analysis also confirmed that after removal of the collar, there was a significant decrease in ICP. Conclusions: Our study suggests that the use of a cervical collar increases ICP in TBI and head injury patients, which may have detrimental effects. However, due to the extremely low sample size from all six studies, caution must be exercised when interpreting these data. Thus, further high-quality research is necessary to unequivocally clarify whether cervical collars should be used in patients with TBI.
SARS-CoV-2 Variants of Concern Infect the Respiratory Tract and Induce Inflammatory Response in Wild-Type Laboratory Mice
Authors: Stone Shannon , Rothan Hussin Alwan , Natekar Janhavi Prasad , Kumari Pratima , Sharma Shaligram , Pathak Heather , Arora Komal , Auroni Tabassum Tasnim , Kumar Mukesh
Published: December 2021

The emergence of new severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants of concern pose a major threat to public health, due to possible enhanced virulence, transmissibility and immune escape. These variants may also adapt to new hosts, in part through mutations in the spike protein. In this study, we evaluated the infectivity and pathogenicity of SARS-CoV-2 variants of concern in wild-type C57BL/6 mice. Six-week-old mice were inoculated intranasally with a representative virus from the original B.1 lineage, or the emerging B.1.1.7 and B.1.351 lineages. We also infected a group of mice with a mouse-adapted SARS-CoV-2 (MA10). Viral load and mRNA levels of multiple cytokines and chemokines were analyzed in the lung tissues on day 3 after infection. Our data show that unlike the B.1 virus, the B.1.1.7 and B.1.351 viruses are capable of infecting C57BL/6 mice and replicating at high concentrations in the lungs. The B.1.351 virus replicated to higher titers in the lungs compared with the B.1.1.7 and MA10 viruses. The levels of cytokines (IL-6, TNF-α, IL-1β) and chemokine (CCL2) were upregulated in response to the B.1.1.7 and B.1.351 infection in the lungs. In addition, robust expression of viral nucleocapsid protein and histopathological changes were detected in the lungs of B.1.351-infected mice. Overall, these data indicate a greater potential for infectivity and adaptation to new hosts by emerging SARS-CoV-2 variants.
Genetic Diversity and Molecular Epidemiology of Circulating Respiratory Syncytial Virus in Central Taiwan, 2008–2017
Authors: Lee Chun-Yi , Fang Yu-Ping , Wang Li-Chung , Chou Teh-Ying , Liu Hsin-Fu
Published: December 2021

In this study, we investigated the molecular evolution and phylodynamics of respiratory syncytial virus (RSV) over 10 consecutive seasons (2008–2017) and the genetic variability of the RSV genotypes ON1 and BA in central Taiwan. The ectodomain region of the G gene was sequenced for genotyping. The nucleotide and deduced amino acid sequences of the second hypervariable region of the G protein in RSV ON1 and BA were analyzed. A total of 132 RSV-A and 81 RSV-B isolates were obtained. Phylogenetic analysis revealed that the NA1, ON1, and BA9 genotypes were responsible for the RSV epidemics in central Taiwan in the study period. For RSV-A, the NA1 genotype predominated during the 2008–2011 seasons. The ON1 genotype was first detected in 2011 and replaced NA1 after 2012. For RSV-B, the BA9 and BA10 genotypes cocirculated from 2008 to 2010, but the BA9 genotype has predominated since 2012. Amino acid sequence alignments revealed the continuous evolution of the G gene in the ectodomain region. The predicted N-glycosylation sites were relatively conserved in the ON1 (site 237 and 318) and BA9 (site 296 and 310) genotype strains. Our results contribute to the understanding and prediction of the temporal evolution of RSV at the local level.
Disseminated Toxoplasma gondii Infection in an Adult Osprey (Pandion haliaetus)
Authors: Wang Xiaobo , Talbot Charles T. , Yin Ji-Hang , Kalalah Anwar A. , Wang Chengming , Newton Joseph C.
Published: December 2021

An adult female osprey (Pandion haliaetus) was found weak and unable to fly in Auburn, Alabama in August 2019. The bird was captured and submitted to the Southeastern Raptor Center of the Auburn University College of Veterinary Medicine for evaluation. On presentation, the bird was thin with a body condition score of approximately 1.5 out of 5. The bird died during the examination and was submitted for necropsy. At the necropsy, there was a severe loss of muscle mass over the body, and the keel was prominent. The liver and spleen were moderately enlarged with pale tan to red foci randomly scattered throughout the parenchyma. A histopathologic observation revealed multifocal to coalescing areas of necrosis and hemorrhage with intralesional protozoans in the liver, spleen, lungs, kidney, sciatic nerve, esophagus, cerebrum, heart, and proventriculus. Immunohistochemistry using anti-Toxoplasma gondii-specific antibodies showed a strong positive labeling of the parasite. Semi-nested PCR, specific for the B1 gene of T. gondii, successfully identified T. gondii. This is the first confirmed case of T. gondii infection in an osprey.
Skeletal Growth Rates in Porites lutea Corals from Pulau Tinggi, Malaysia
Authors: Ong Chai Kee , Lee Jen Nie , Tanzil Jani Thuaibah Isa
Published: December 2021

Skeletal records of massive Porites lutea corals sampled from reefs around Malaysia have previously shown average decadal declines in growth rates associated with sea warming. However, there was a variability in growth declines between sites that warrant the need for investigations into more site-specific variations. This study analyzed decade-long (December 2004–November 2014) annual growth records (annual linear extension rate, skeletal bulk density, calcification rate) reconstructed from five massive P. lutea colonies from Pulau Tinggi, Malaysia. Significant non-linear changes in inter-annual trends of linear extension and calcification rates were found, with notable decreases that corresponded to the 2010 El Niño thermal stress episode and a pan-tropical mass coral bleaching event. Coral linear extension and calcification were observed to return to pre-2010 rates by 2012, suggesting the post-stress recovery of P. lutea corals at the study site within 2 years. Although no long-term declines in linear extension and calcification rates were detected, a linear decrease in annual skeletal bulk density by ≈9.5% over the 10-year study period was found. This suggests that although coral calcification rates are retained, the skeletal integrity of P. lutea corals may be compromised with potential implications for the strength of the overall reef carbonate framework. The correlation of coral calcification rates with sea surface temperature also demonstrated site-specific thermal threshold at 29 °C, which is comparable to the regional thermal threshold previously found for the Thai-Malay Peninsula.
Trend and Attribution Analysis of Runoff Changes in the Weihe River Basin in the Last 50 Years
Authors: Xu Junjie , Gao Xichao , Yang Zhiyong , Xu Tianyin
Published: December 2021

In recent years, the Weihe River basin has experienced dramatic changes and a sharp decrease in runoff, which has constrained the sustainable development of the local society, economy, and ecology. Quantitative attribution analysis of runoff changes in the Weihe River basin can help to illustrate reasons for dramatic runoff changes and to understand its complex hydrological response. In this paper, the trends of hydrological elements in the Weihe River basin from 1970 to 2019 were systematically analyzed using the M–K analysis method, and the effects of meteorological elements and underlying surface changes on runoff were quantitatively analyzed using the Budyko theoretical framework. The results show that potential evapotranspiration and precipitation in the Weihe River basin have no significant change in 1970–2019; runoff depth has an abrupt change around 1990 and then decrease significantly. The study period is divided into the base period (1970–1989), PΙ (1990–2009), and PII (2010–2019). Compared with the base period, the elasticity coefficients (absolute values) of each element show an increasing trend in PΙ and PII. The sensitivity of runoff to these coefficients is increasing. The sensitivity of the precipitation is the highest (2.72~3.17), followed by that of the underlying surface parameter (−2.01~−2.35); the sensitivity of the potential evapotranspiration is the weakest (−1.72~−2.17). In the PΙ period, the runoff depth decreased significantly due to the combination effects of precipitation and underlying surface with the values of 6.18 mm and 13.92 mm, respectively. In the PII period, rainfall turned to an increasing trend, contributing to the increase in runoff by 11.80 mm; the further increase in underlying surface parameters was the main reason for the decrease in runoff by 22.19 mm. The significant increase in runoff by 8.54 mm because of the increased rainfall, compared with the PΙ periods. Overall, the increasing underlying surface parameter makes the largest contribution to the runoff changes while the precipitation change is also an important factor.