bluuebunny/crossref_metadata_2025 huggingface dataset:
A Mental Health Pandemic? Assessing the Impact of COVID-19 on Young People’s Mental Health
Authors: Lloyd Katrina , Schubotz Dirk , Roche Rosellen , Manzi Joel , McKnight Martina
Published: August 2023

Background: Research indicates that young people have been a particularly vulnerable group when it comes to negative mental health outcomes following COVID-19, with some authors warning of a ‘mental health pandemic’. Materials and Method: Using a survey approach, this study explored the effects of lockdowns on the mental health of 1995 16-year-olds in Northern Ireland. Respondents completed the 12-item version of the General Health Questionnaire (GHQ-12) along with closed- and open-ended questions about COVID-19. Results: Results from regression analysis showed that being female, identifying as non-heterosexual and perceiving that mental health had worsened during lockdown were the best predictors of poor mental health. In the open responses, young people reported significant concerns about their mental health and their educational outcomes. They also felt that their needs were not given the same priority as those of adults during lockdown. Conclusions: The results suggest that the COVID-19 lockdowns adversely affected the mental health of many young people in Northern Ireland with the effects most acute for females and those identifying as non-heterosexual. Future research should explore the longer-term impact of the pandemic on the mental health of these vulnerable young people and identify what support mechanisms need to be put in place to mitigate the negative effects of any future crises.
Occupational Exposure to Electromagnetic Fields—Different from General Public Exposure and Laboratory Studies
Authors: Hansson Mild Kjell , Mattsson Mats-Olof , Jeschke Peter , Israel Michel , Ivanova Mihaela , Shalamanova Tsvetelina
Published: August 2023

The designs of in vivo, in vitro and in silico studies do not adequately reflect the characteristics of long-term occupational EMF exposure; the higher exposure levels permitted for employees are nevertheless extrapolated on this basis. Epidemiological studies consider occupational exposure only in a very general way, if at all. There is a lack of detailed descriptive data on long-term occupational exposure over the duration of the working life. Most studies reflect exposure characteristics of the general population, exposures which are long-term, but at a comparably low level. Occupational exposure is often intermittent with high peak power followed by periods with no exposure. Furthermore, the EU EMF-Directive 2013/35/EU states a demand for occupational health surveillance, the outcome of which would be of great help to epidemiologists studying the health effects of EMF exposure. This paper thus aims to outline and specify differences between public and occupational exposure and to increase the understanding of specific aspects of occupational exposure which are important for long-term health considerations. This could lead to a future protection concept against possible hazards based on adequate descriptions of long-term exposures and also include supplementary descriptive features such as a “reset time” of biological systems and accurate dose quantities.
An Improved Adaptive Sparrow Search Algorithm for TDOA-Based Localization
Authors: Dong Jiaqi , Lian Zengzeng , Xu Jingcheng , Yue Zhe
Published: August 2023

The Ultra-Wideband (UWB) indoor positioning method is widely used in areas where no satellite signals are available. However, during the measurement process of UWB, the collected data contain random errors. To alleviate the effect of random errors on positioning accuracy, an improved adaptive sparrow search algorithm (IASSA) based on the sparrow search algorithm (SSA) is proposed in this paper by introducing three strategies, namely, the two-step weighted least squares algorithm, adaptive adjustment of search boundary, and producer–scrounger quantity adaptive adjustment. The simulation and field test results indicate that the IASSA algorithm achieves significantly higher localization accuracy than previous methods. Meanwhile, the IASSA algorithm requires fewer iterations, which overcomes the problem of the long computation time of the swarm intelligence optimization algorithm. Therefore, the IASSA algorithm has advantages in indoor positioning accuracy and robustness performance.
Anticancer Study of a Novel Pan-HDAC Inhibitor MPT0G236 in Colorectal Cancer Cells
Authors: Tsai Feng-Lung , Huang Han-Li , Lai Mei-Jung , Liou Jing-Ping , Pan Shiow-Lin , Yang Chia-Ron
Published: August 2023

Colorectal cancer (CRC) is one of the most commonly diagnosed malignancies and a leading cause of cancer worldwide. Histone deacetylases (HDACs), which regulate cell proliferation and survival, are associated with the development and progression of cancer. Moreover, HDAC inhibitors are promising therapeutic targets, with five HDAC inhibitors approved for cancer treatment to date. However, their safety profile necessitates the exploration of well-tolerated HDAC inhibitors that can be used in cancer therapeutic strategies. In this study, the pan-HDAC inhibitor MPT0G236 reduced the viability and inhibited the proliferation of human colorectal cancer cells, and normal human umbilical vein endothelial cells (HUVECs) showed reduced sensitivity. These findings indicated that MPT0G236 specifically targeted malignant tumor cells. Notably, MPT0G236 significantly inhibited the activities of HDAC1, HDAC2, and HDAC3, Class I HDACs, as well as HDAC6, a Class IIb HDAC, at low nanomolar concentrations. Additionally, it promoted the accumulation of acetyl-α-tubulin and acetyl-histone H3 in HCT-116 and HT-29 cells in a concentration-dependent manner. Furthermore, MPT0G236 treatment induced G2/M cell cycle arrest in CRC cells by initially regulating the levels of cell-cycle-related proteins, such as p-MPM2; specifically reducing p-cdc2 (Y15), cyclin B1, and cdc25C levels; and subsequently inducing apoptosis through the caspase-dependent pathways and PARP activation. Our findings demonstrate that MPT0G236 exhibits significant anticancer activity in human colorectal cancer cells.
The First Five Mitochondrial Genomes for the Family Nidulariaceae Reveal Novel Gene Rearrangements, Intron Dynamics, and Phylogeny of Agaricales
Authors: Li Zhao-chen , Xie Tian-chen , Feng Xi-long , Wang Zhen-xin , Lin Chao , Li Guo-ming , Li Xiu-Zhang , Qi Jianzhao
Published: August 2023

The family Nidulariaceae, consisting of five genera including Cyathus, is a unique group of mushrooms commonly referred to as bird’s nest fungi due to their striking resemblance to bird’s nests. These mushrooms are considered medicinal mushrooms in Chinese medicine and have received attention in recent years for their anti-neurodegenerative properties. However, despite the interest in these mushrooms, very little is known about their mitochondrial genomes (mitogenomes). This study is the first comprehensive investigation of the mitogenomes of five Nidulariaceae species with circular genome structures ranging in size from 114,236 bp to 129,263 bp. Comparative analyses based on gene content, gene length, tRNA, and codon usage indicate convergence within the family Nidulariaceae and heterogeneity within the order Agaricales. Phylogenetic analysis based on a combined mitochondrial conserved protein dataset provides a well-supported phylogenetic tree for the Basidiomycetes, which clearly demonstrates the evolutionary relationships between Nidulariaceae and other members of Agaricales. Furthermore, phylogenetic inferences based on four different gene sets reveal the stability and proximity of evolutionary relationships within Agaricales. These results reveal the uniqueness of the family Nidulariaceae and its similarity to other members of Agaricales; provide valuable insights into the origin, evolution, and genetics of Nidulariaceae species; and enrich the fungal mitogenome resource. This study will help to expand the knowledge and understanding of the mitogenomes in mushrooms.
S-Adenosylhomocysteine Is a Useful Metabolic Factor in the Early Prediction of Septic Disease Progression and Death in Critically Ill Patients: A Prospective Cohort Study
Authors: Centner Franz-Simon , Schoettler Jochen J. , Brohm Kathrin , Mindt Sonani , Jäger Evelyn , Hahn Bianka , Fuderer Tanja , Lindner Holger A. , Schneider-Lindner Verena , Krebs Joerg , Neumaier Michael , Thiel Manfred
Published: August 2023

A common final pathway of pathogenetic mechanisms in septic organ dysfunction and death is a lack or non-utilization of oxygen. Plasma concentrations of lactate serve as surrogates for the oxygen-deficiency-induced imbalance between energy supply and demand. As S-adenosylhomocysteine (SAH) was shown to reflect tissue hypoxia, we compared the ability of SAH versus lactate to predict the progression of inflammatory and septic disease to septic organ dysfunction and death. Using univariate and multiple logistic regression, we found that SAH but not lactate, taken upon patients’ inclusion in the study close to ICU admission, significantly and independently contributed to the prediction of disease progression and death. Due to the stronger increase in SAH in relation to S-adenosylmethionine (SAM), the ratio of SAM to SAH, representing methylation potential, was significantly decreased in patients with septic organ dysfunction and non-survivors compared with SIRS/sepsis patients (2.8 (IQR 2.3–3.9) vs. 8.8 (4.9–13.8); p = 0.003) or survivors (4.9 (2.8–9.5) vs. 8.9 (5.1–14.3); p = 0.026), respectively. Thus, SAH appears to be a better contributor to the prediction of septic organ dysfunction and death than lactate in critically ill patients. As SAH is a potent inhibitor of SAM-dependent methyltransferases involved in numerous vital biochemical processes, the impairment of the SAM-to-SAH ratio in severely critically ill septic patients and non-survivors warrants further studies on the pathogenetic role of SAH in septic multiple organ failure.
The Proteome Profile of Olfactory Ecto-Mesenchymal Stem Cells-Derived from Patients with Familial Alzheimer’s Disease Reveals New Insights for AD Study
Authors: Rochín-Hernández Lory , Jiménez-Acosta Miguel , Ramírez-Reyes Lorena , Figueroa-Corona María , Sánchez-González Víctor , Orozco-Barajas Maribel , Meraz-Ríos Marco
Published: August 2023

Alzheimer’s disease (AD), the most common neurodegenerative disease and the first cause of dementia worldwide, has no effective treatment, and its pathological mechanisms are not yet fully understood. We conducted this study to explore the proteomic differences associated with Familial Alzheimer’s Disease (FAD) in olfactory ecto-mesenchymal stem cells (MSCs) derived from PSEN1 (A431E) mutation carriers compared with healthy donors paired by age and gender through two label-free liquid chromatography-mass spectrometry approaches. The first analysis compared carrier 1 (patient with symptoms, P1) and its control (healthy donor, C1), and the second compared carrier 2 (patient with pre-symptoms, P2) with its respective control cells (C2) to evaluate whether the protein alterations presented in the symptomatic carrier were also present in the pre-symptom stages. Finally, we analyzed the differentially expressed proteins (DEPs) for biological and functional enrichment. These proteins showed impaired expression in a stage-dependent manner and are involved in energy metabolism, vesicle transport, actin cytoskeleton, cell proliferation, and proteostasis pathways, in line with previous AD reports. Our study is the first to conduct a proteomic analysis of MSCs from the Jalisco FAD patients in two stages of the disease (symptomatic and presymptomatic), showing these cells as a new and excellent in vitro model for future AD studies.
Canonical Transient Receptor Potential Channel 3 Contributes to Cerebral Blood Flow Changes Associated with Cortical Spreading Depression in Mice
Authors: Zheng Fang
Published: August 2023

Cortical spreading depression is a pathophysiological event shared in migraines, strokes, traumatic brain injuries, and epilepsy. It is associated with complex hemodynamic responses, which, in turn, contribute to neurological problems. In this study, we investigated the role of canonical transient receptor potential channel 3 (TRPC3) in the hemodynamic responses elicited by cortical spreading depression. Cerebral blood flow was monitored using laser speckle contrast imaging, and cortical spreading depression was triggered using three well-established experimental approaches in mice. A comparison of TRPC3 knockout mice to controls revealed that the genetic ablation of TRPC3 expression significantly altered the hemodynamic responses elicited using cortical spreading depression and promoted hyperemia consistently. Our results indicate that TRPC3 contributes to hemodynamic responses associated with cortical spreading depression and could be a novel therapeutic target for a host of neurological disorders.
The Future of Precision Oncology
Authors: Rulten Stuart L. , Grose Richard P. , Gatz Susanne A. , Jones J. Louise , Cameron Angus J. M.
Published: August 2023

Our understanding of the molecular mechanisms underlying cancer development and evolution have evolved rapidly over recent years, and the variation from one patient to another is now widely recognized. Consequently, one-size-fits-all approaches to the treatment of cancer have been superseded by precision medicines that target specific disease characteristics, promising maximum clinical efficacy, minimal safety concerns, and reduced economic burden. While precision oncology has been very successful in the treatment of some tumors with specific characteristics, a large number of patients do not yet have access to precision medicines for their disease. The success of next-generation precision oncology depends on the discovery of new actionable disease characteristics, rapid, accurate, and comprehensive diagnosis of complex phenotypes within each patient, novel clinical trial designs with improved response rates, and worldwide access to novel targeted anticancer therapies for all patients. This review outlines some of the current technological trends, and highlights some of the complex multidisciplinary efforts that are underway to ensure that many more patients with cancer will be able to benefit from precision oncology in the near future.
Repurposing of World-Approved Drugs for Potential Inhibition against Human Carbonic Anhydrase I: A Computational Study
Authors: Zheng Nannan , Jiang Wanyun , Zhang Puyu , Ma Le , Chen Junzhao , Zhang Haiyang
Published: August 2023

Human carbonic anhydrases (hCAs) have enzymatic activities for reversible hydration of CO2 and are acknowledged as promising targets for the treatment of various diseases. Using molecular docking and molecular dynamics simulation approaches, we hit three compounds of methyl 4-chloranyl-2-(phenylsulfonyl)-5-sulfamoyl-benzoate (84Z for short), cyclothiazide, and 2,3,5,6-tetrafluoro-4-piperidin-1-ylbenzenesulfonamide (3UG for short) from the existing hCA I inhibitors and word-approved drugs. As a Zn2+-dependent metallo-enzyme, the influence of Zn2+ ion models on the stability of metal-binding sites during MD simulations was addressed as well. MM-PBSA analysis predicted a strong binding affinity of −18, −16, and −14 kcal/mol, respectively, for these compounds, and identified key protein residues for binding. The sulfonamide moiety bound to the Zn2+ ion appeared as an essential component of hCA I inhibitors. Vina software predicted a relatively large (unreasonable) Zn2+–sulfonamide distance, although the relative binding strength was reproduced with good accuracy. The selected compounds displayed potent inhibition against other hCA isoforms of II, XIII, and XIV. This work is valuable for molecular modeling of hCAs and further design of potent inhibitors.
A New Image Analysis Assisted Semi-Automatic Geometrical Measurement of Fibers in Thermoplastic Composites: A Case Study on Giant Reed Fibers
Authors: Suárez Luis , Billham Mark , Garrett Graham , Cunningham Eoin , Marrero María Dolores , Ortega Zaida
Published: August 2023

This work describes a systematic method for the analysis of the attrition and residual morphology of natural fibers during the compounding process by twin-screw extrusion. There are several methods for the assessment of fiber lengths and morphology, although they are usually based on the use of non-affordable apparatus or time-consuming methods. In this research, the variation of morphological features such as the length, diameter and aspect ratio of natural fibers were analyzed by affordable optical scanning methods and open-source software. This article presents the different steps to perform image acquisition, refining and measurement in an automated way, achieving statistically representative results, with thousands of fibers analyzed per scanned sample. The use of this technique for the measurement of giant reed fibers in polyethylene (PE) and polylactide (PLA)-based composite materials has proved that there are no significant differences in the output fiber morphology of the compound, regardless of the fiber feed sizes, extruder scale, or the polymer used as matrix. The ratio of fiber introduced for the production of composites also did not significantly affect the final fiber size. The greatest reduction in size was obtained in the first kneading zone during compounding. Pelletizing or injection molding did not significantly modify the fiber size distribution.
Candida auris in Intensive Care Setting: The First Case Reported in Portugal
Authors: Henriques João , Mixão Verónica , Cabrita Joana , Duarte Tiago Isidoro , Sequeira Tânia , Cardoso Sofia , Germano Nuno , Dias Liliana , Bento Luís , Duarte Sílvia , Veríssimo Cristina , Gomes João Paulo , Sabino Raquel
Published: August 2023

Candida auris is an opportunistic human pathogen that has rapidly spread to multiple countries and continents and has been associated with a high number of nosocomial outbreaks. Herein, we report the first case of C. auris in Portugal, which was associated with a patient transferred from Angola to an ICU in Portugal for liver transplantation after a SARS-CoV-2 infection. C. auris was isolated during the course of bronchoalveolar lavage, and it was subjected to antifungal susceptibility testing and whole-genome sequence analysis. This isolate presents low susceptibility to azoles and belongs to the genetic clade III with a phylogenetic placement close to African isolates. Although clade III has already been reported in Europe, taking into account the patient’s clinical history, we cannot discard the possibility that the patient’s colonization/infection occurred in Angola, prior to admission in the Portuguese hospital. Considering that C. auris is a fungal pathogen referenced by WHO as a critical priority, this case reinforces the need for continuous surveillance in a hospital setting.
Characterizing 3T3-L1 MBX Adipocyte Cell Differentiation Maintained with Fatty Acids as an In Vitro Model to Study the Effects of Obesity
Authors: Mubtasim Noshin , Gollahon Lauren
Published: August 2023

The increasing prevalence of obesity has prompted intensive research into understanding its role in pathogenesis and designing appropriate treatments. To determine the signals generated from the interaction of fat cells with a target organ, a reliable white adipocyte model in vitro is needed. Differentiated fibroblasts are the most extensively studied using in vitro cell models of white adipocytes. However, it can be argued that differentiated fibroblasts minimally recapitulate the consequences of obesity. Here, we describe 3T3-L1 MBX cells as a culture model for studying obese adipocytes and their effects. Differentiation of 3T3-L1 MBX cells was at first optimized and then maintained in the presence of fatty acids cocktail combination to induce the obese condition. Lipid accumulation and adipokine secretion profiles were analyzed. Results showed that fatty acid-maintained, differentiated 3T3-L1 MBX cells had significantly greater accumulation of lipids and significant changes in the adipokine secretions in comparison to differentiated 3T3-L1 MBX cells maintained in medium without fatty acids. To elucidate the molecular changes associated with adipogenesis and lipid accumulation profile of 3T3-L1 MBX cells, we have also explored the expression of some of the regulatory proteins related to the development and maintenance of adipocytes from the preadipocyte lineage.
Tributyl(1-((dimethylamino)(dimethyliminio)methyl)-1,4-dihydropyridin-4-yl)phosphonium Ditrifluoromethanesulfonate
Authors: Gong Yiwei , Ward Jas S. , Rissanen Kari , Mulks Florian F.
Published: August 2023

Site-selective functionalization of pyridines is a crucial tool for the synthesis of diverse pharmaceuticals and materials. We introduced diiminium pyridine adducts as highly convenient and potent Lewis acids. We report that tributylphosphine selectively adds to the 4-position of pyridine in tetramethyldiiminium pyridine ditrifluoromethanesulfonate, resulting in the formation of the title compound. This finding represents an advancement towards the utilization of diiminium units as organic reagents or catalysts for pyridine functionalization. We also employ computational models to determine fluoride and hydride ion affinities, Fukui function f+(r), molecular electrostatic potential, and pKa values, providing valuable insights for future investigations in this area.
The Effect of Annealing on the Soft Magnetic Properties and Microstructure of Fe82Si2B13P1C3 Amorphous Iron Cores
Authors: Zheng Wei , Zhang Guangqiang , Zhang Qian , Yu Haichen , Li Zongzhen , Gu Mingyu , Song Su , Zhou Shaoxiong , Qu Xuanhui
Published: August 2023

This research paper investigated the impact of normal annealing (NA) and magnetic field annealing (FA) on the soft magnetic properties and microstructure of Fe82Si2B13P1C3 amorphous alloy iron cores. The annealing process involved various methods of magnetic field application: transverse magnetic field annealing (TFA), longitudinal magnetic field annealing (LFA), transverse magnetic field annealing followed by longitudinal magnetic field annealing (TLFA) and longitudinal magnetic field annealing followed by transverse magnetic field annealing (LTFA). The annealed samples were subjected to testing and analysis using techniques such as differential scanning calorimetry (DSC), transmission electron microscopy (TEM), X-ray diffraction (XRD), magnetic performance testing equipment and magneto-optical Kerr microscopy. The obtained results were then compared with those of commercially produced Fe80Si9B11. Fe82Si2B13P1C3 demonstrated the lowest loss of P1.4T,2kHz = 8.1 W/kg when annealed in a transverse magnetic field at 370 °C, which was 17% lower than that of Fe80Si9B11. When influenced by the longitudinal magnetic field, the magnetization curve tended to become more rectangular, and the coercivity (B3500A/m) of Fe82Si2B13P1C3 reached 1.6 T, which was 0.05 T higher than that of Fe80Si9B11. During the 370 °C annealing process of the Fe82Si2B13P1C3 amorphous iron core, the internal stress in the strip gradually dissipated, and impurity domains such as fingerprint domains disappeared and aligned with the length direction of the strip. Consequently, wide strip domains with low resistance and easy magnetization were formed, thereby reducing the overall loss of the amorphous iron core.
Enhanced Thermoelectric Properties of Nb-Doped Ti(FeCoNi)Sb Pseudo-Ternary Half-Heusler Alloys Prepared Using the Microwave Method
Authors: Zhang Ruipeng , Kong Jianbiao , Hou Yangbo , Zhao Linghao , Zhu Junliang , Li Changcun , Zhao Degang
Published: August 2023

Pseudo-ternary half-Heusler thermoelectric materials, which are formed by filling the B sites of traditional ternary half-Heusler thermoelectric materials of ABX with equal atomic proportions of various elements, have attracted more and more attention due to their lower intrinsic lattice thermal conductivity. High-purity and relatively dense Ti1−xNbx(FeCoNi)Sb (x = 0, 0.01, 0.03, 0.05, 0.07 and 0.1) alloys were prepared via microwave synthesis combined with rapid hot-pressing sintering, and their thermoelectric properties are investigated in this work. The Seebeck coefficient was markedly increased via Nb substitution at Ti sites, which resulted in the optimized power factor of 1.45 μWcm−1K−2 for n-type Ti0.93Nb0.07(FeCoNi)Sb at 750 K. In addition, the lattice thermal conductivity was largely decreased due to the increase in phonon scattering caused by point defects, mass fluctuation and strain fluctuation introduced by Nb-doping. At 750 K, the lattice thermal conductivity of Ti0.97Nb0.03(FeCoNi)Sb is 2.37 Wm−1K−1, which is 55% and 23% lower than that of TiCoSb and Ti(FeCoNi)Sb, respectively. Compared with TiCoSb, the ZT of the Ti1−xNbx(FeCoNi)Sb samples were significantly increased. The average ZT values of the Nb-doped pseudo-ternary half-Heusler samples were dozens of times that of the TiCoSb prepared using the same process.
Improving the Properties of Gray Cast Iron by Laser Surface Modification
Authors: Sai Qingyi , Hao Jiale , Wang Shuwen , Wang Zhi
Published: August 2023

Laser surface modification is a widely used technology to improve the properties of functional surfaces. In this study, the properties of gray cast iron are modified by laser surface modification, and the influence of laser quenching on the properties of cast iron in terms of frictional vibration and noise, friction and wear, internal structure, residual stress, hardness, and corrosion resistance is investigated. The experimental results show that, after high-power laser quenching, the frictional vibrations and noise of most gray cast iron specimens are decreased, but the coefficients of friction against a bearing steel counterface are increased and more stable. The surface and sub-surface hardness of all laser-quenched cast iron specimens is significantly increased. The residual stresses on the surface of the cast iron specimens are significantly increased and changed from tensile to compressive residual stresses. Experimental modal testing results show that the modal damping ratios of the laser-treated specimens are increased significantly, although their modal frequencies are not significantly changed. In addition, through the metallographic observation, XRD (X-ray diffraction) analysis, and TEM (transmission electron microscopy) observation, it is found that the microstructures of the cast iron specimen after high-power laser modification become fine-grained, and the pearlite and ferrite in the matrix become fine martensite, which leads to the improvement of the dynamical, tribological, and chemical properties of cast iron after laser modification.
Progress in the Synthesis and Application of Transparent Conducting Film of AZO (ZnO:Al)
Authors: Zhang Dingyi , Yu Wenhe , Zhang Lu , Hao Xiangyang
Published: August 2023

Due to the excellent performance and low cost of the new aluminum-doped zinc oxide (AZO) film, it is expected to replace the mature indium-doped tin oxide (ITO) film. The research status and progress of AZO transparent conductive films are summarized in this review. Moreover, the structure, optoelectronic properties, and conductive mechanism of AZO thin films are also detailed. The thin films’ main preparation processes and the advantages and disadvantages of each process method are mainly discussed, and their application fields are expounded. AZO thin films with multicomponent composite structures are one of the promising development directions in transparent conductive oxide (TCO) thin films. The development of various preparation processes has promoted the production and application of thin films on a broad scale. Finally, some improvement schemes have been proposed to improve the comprehensive performance of the film. The industrialization prospects of the AZO film, as well as its great development potential in the digital world, are discussed.
Drying Kinetics of Microwave-Assisted Drying of Leaching Residues from Hydrometallurgy of Zinc
Authors: Tian Chunlan , Zhou Ju , Ren Chunxiao , Omran Mamdouh , Zhang Fan , Tang Ju
Published: August 2023

In the hydrometallurgical process of zinc production, the residue from the leaching stage is an important intermediate product and is treated in a Waelz kiln to recover valuable metals. To ensure optimal results during the Waelz kiln process, it is necessary to pre-treat the residues by drying them first due to their higher water content. This work studies the residue’s drying process using microwave technology. The study results indicate that microwave technology better removes the residue’s oxygen functional groups and moisture. The dehydration process’s effective diffusion coefficient increases as the microwave’s heating power, the initial moisture content, and the initial mass increase. The Page model is appropriate for imitating the drying process, and the activation energy of the drying process for the residues is −13.11217 g/W. These results indicate that microwave technology efficiently dries the residues from the leaching stage. Furthermore, this study provides a theoretical basis and experimental data for the industrial application of microwave drying.
A Symbolic Approach to Discrete Structural Optimization Using Quantum Annealing
Authors: Wils Kevin , Chen Boyang
Published: August 2023

With the advent of novel quantum computing technologies and the new possibilities thereby offered, a prime opportunity has presented itself to investigate the practical application of quantum computing. This work investigates the feasibility of using quantum annealing for structural optimization. The target problem is the discrete truss sizing problem—the goal is to select the best size for each truss member so as to minimize a stress-based objective function. To make the problem compatible with quantum annealing devices, the objective function must be translated into a quadratic unconstrained binary optimization (QUBO) form. This work focuses on exploring the feasibility of making this translation. The practicality of using a quantum annealer for such optimization problems is also assessed. A method is eventually established to translate the objective function into a QUBO form and have it solved by a quantum annealer. However, scaling the method to larger problems faces some challenges that would require further research to address.
On a New Approach for Stability and Controllability Analysis of Functional Equations
Authors: Aderyani Safoura Rezaei , Saadati Reza , O’Regan Donal , Li Chenkuan
Published: August 2023

We consider a new approach to approximate stability analysis for a tri-additive functional inequality and to obtain the optimal approximation for permuting tri-derivations and tri-homomorphisms in unital matrix algebras via the vector-valued alternative fixed-point theorem, which is a popular technique of proving the stability of functional equations. We also present a small list of aggregation functions on the classical, well-known special functions to investigate the best approximation error estimates using a different concept of perturbation stability.
Risk Assessment Analysis of Multiple Failure Modes Using the Fuzzy Rough FMECA Method: A Case of FACDG
Authors: Yan Yutao , Luo Zhongqiang , Liu Zhenyu , Liu Zhibo
Published: August 2023

With the increasing operating mileage and ownership of high-speed electric multiple units (EMU), a reasonable operation and maintenance strategy is the key to ensure their safe and reliable operation. As a key component of recombined EMU, creating a reasonable and effective risk assessment method for the fully automatic coupler draft gear (FACDG) is the first task. Therefore, based on fuzzy rough number theory, combined with the analytic hierarchy process (AHP), entropy weight method (EWM), technique for order performance by similarity to ideal solution (TOPSIS) and grey relational analysis (GRA), a risk priority indicator of comprehensive nearness degree is developed. Furthermore, a novel multi-criteria decision making (MCDM) failure modes, effects and criticality analysis (FMECA) assessment method is proposed. The effectiveness and rationality of the risk assessment method proposed are verified by the analysis of data and failure modes of a certain FACDG at fourth-level engineering maintenance.
Spectrum of Thyroid Dysfunction in Patients with Chronic Kidney Disease in Benin City, Nigeria
Authors: Obasuyi John O. , Emokpae Mathias A.
Published: August 2023

There is an indication of abrupt rise in chronic kidney disease (CKD) in Nigeria and thyroid function involvement has not been sufficiently evaluated. This study determined thyroid gland function among subjects with CKD in Benin City, Nigeria. A total of 184 randomized CKD patients attending specialist clinic and 80 healthy control subjects were recruited for this study. A well-structured questionnaire was used to obtain data on socio-demography. Blood specimens were collected and used for the determination of thyroid function parameters; thyroid stimulating hormone (TSH), triiodothyronine (T3), free triiodothyronine (fT3), thyroxine (T4), free thyroxine (fT4), thyroid peroxidase antibody (TPO-Abs), thyroid globulin antibody (Tg-Abs) and Deiodinase enzyme Type 1 (D1). SPINA GD and SPINA GT were calculated using Michaelis-Menten model. The CKD was classified into stages using Modification of Drug in Renal Disease (MDRD) formula. Thyroid dysfunctions observed were clinical hyperthyroidism 1 (0.54%), non-thyroidal illness 78 (42.4%), clinical hypothyroidism 11 (6.0%), sub-clinical hyperthyroidism 3 (1.60%), and sub-clinical hypothyroidism 11 (6.0%), while euthyroid were 80 (43.5%). SPINA GD of CKD patients (33.85 ± 10.94) was not significantly different when compared with controls (24.85 ± 1.57), whereas, SPINA GT was significantly higher (p < 0.01) among CKD patients (3.74 ± 0.31) than controls (2.68 ± 0.11). Autoimmune thyroid disease demonstrated by positive Tg-Abs and TPO-Abs were observed among approximately 7.9% of CKD patients. Serum TPO-Abs concentration increased with CKD progression. Thyroid dysfunction is involved in the pathogenesis of CKD patients. The etiologies are multifactorial and immunological mechanisms of autoimmune thyroid disease may be a contributing factor.
Electrochemical Determination of Ascorbic Acid by Mechanically Alloyed Super Duplex Stainless Steel Powders
Authors: Mahale Rayappa Shrinivas , Vasanth Shamanth , Chikkegouda Sharath Peramenahalli , Rajendrachari Shashanka , Narsimhachary Damanapeta , Basavegowda Nagaraj
Published: August 2023

SAF-2507 super duplex stainless steel powders (SDSS) were prepared using a high-energy planetary ball milling process. The X-ray diffraction (XRD) shows peak broadening after 20 h of ball milling and revealed a phase transformation resulting in a two-phase alloy mixture containing nearly equal amounts of ferrite (α) and austenite (γ). After 20 h of ball milling the particle size was reduced to ~201 nm. Scanning electron microscope (SEM) micrographs showed small-size irregular grains with an average particle size ranging from 5–7 µm. The high-resolution transmission microscope (HRTEM) analysis confirmed the presence of nanocrystalline particles with sizes ranging from 10 to 50 nm. The presence of ferrite phase is visible in the corresponding diffraction pattern as well. In this paper, we have discussed the electrochemical sensor application of mechanically alloyed nano-structured duplex stainless steel powders. The fabricated 4 mg duplex stainless steel modified carbon paste electrode (SDSS-MCPE) has shown excellent current sensitivity in comparison with 2, 6, 8, and 10 mg SDSS-MCPEs during the detection of ascorbic acid (AA) in a phosphate buffer solution with a pH of 6.8. The calculated electrode active surface area of SDSS-MCPE was found to be almost two times larger than the surface area of the bare carbon paste electrode (BCPE). The limit of detection (LD) and limit of quantification (LQ) were found to be 0.206 × 10−8 M and 0.688 × 10−8 M, respectively, for the fabricated 4 mg SDSS-MCPE.
Current State of Computational Modeling of Nanohelicenes
Authors: Porsev Vitaly V. , Evarestov Robert A.
Published: August 2023

This review considers the works that focus on various aspects of the theoretical description of nanohelicenes (other equivalent names are graphene spirals, graphene helicoid, helical graphene nanoribbon, or helical graphene)—a promising class of one-dimensional nanostructures. The intrinsic helical topology and continuous π-system lead to the manifestation of unique optical, electronic, and magnetic properties that are also highly dependent on axial and torsion strains. In this paper, it was shown that the properties of nanohelicenes are mainly associated with the peripheral modification of the nanohelicene ribbon. We have proposed a nomenclature that enables the classification of all nanohelicenes as modifications of some prototype classes.
Light-Based Anti-Biofilm and Antibacterial Strategies
Authors: Kauser Ambreen , Parisini Emilio , Suarato Giulia , Castagna Rossella
Published: August 2023

Biofilm formation and antimicrobial resistance pose significant challenges not only in clinical settings (i.e., implant-associated infections, endocarditis, and urinary tract infections) but also in industrial settings and in the environment, where the spreading of antibiotic-resistant bacteria is on the rise. Indeed, developing effective strategies to prevent biofilm formation and treat infections will be one of the major global challenges in the next few years. As traditional pharmacological treatments are becoming inadequate to curb this problem, a constant commitment to the exploration of novel therapeutic strategies is necessary. Light-triggered therapies have emerged as promising alternatives to traditional approaches due to their non-invasive nature, precise spatial and temporal control, and potential multifunctional properties. Here, we provide a comprehensive overview of the different biofilm formation stages and the molecular mechanism of biofilm disruption, with a major focus on the quorum sensing machinery. Moreover, we highlight the principal guidelines for the development of light-responsive materials and photosensitive compounds. The synergistic effects of combining light-triggered therapies with conventional treatments are also discussed. Through elegant molecular and material design solutions, remarkable results have been achieved in the fight against biofilm formation and antibacterial resistance. However, further research and development in this field are essential to optimize therapeutic strategies and translate them into clinical and industrial applications, ultimately addressing the global challenges posed by biofilm and antimicrobial resistance.
Cardiomyocyte-Targeting Peptide to Deliver Amiodarone
Authors: Zahid Maliha , Weber Beth , Yurko Ray , Islam Kazi , Agrawal Vaishavi , Lopuszynski Jack , Yagi Hisato , Salama Guy
Published: August 2023

Background: Amiodarone is underutilized due to significant off-target toxicities. We hypothesized that targeted delivery to the heart would lead to the lowering of the dose by utilizing a cardiomyocyte-targeting peptide (CTP), a cell-penetrating peptide identified by our prior phage display work. Methods: CTP was synthesized thiolated at the N-terminus, conjugated to amiodarone via Schiff base chemistry, HPLC purified, and confirmed with MALDI/TOF. The stability of the conjugate was assessed using serial HPLCs. Guinea pigs (GP) were injected intraperitoneally daily with vehicle (7 days), amiodarone (7 days; 80 mg/kg), CTP–amiodarone (5 days; 26.3 mg/kg), or CTP (5 days; 17.8 mg/kg), after which the GPs were euthanized, and the hearts were excised and perfused on a Langendorff apparatus with Tyrode’s solution and blebbistatin (5 µM) to minimize the contractions. Voltage (RH237) and Ca2+-indicator dye (Rhod-2/AM) were injected, and fluorescence from the epicardium split and was captured by two cameras at 570–595 nm for the cytosolic Ca2+ and 610–750 nm wavelengths for the voltage. Subsequently, the hearts were paced at 250 ms with programmed stimulation to measure the changes in the conduction velocities (CV), action potential duration (APD), and Ca2+ transient durations at 90% recovery (CaTD90). mRNA was extracted from all hearts, and RNA sequencing was performed with results compared to the control hearts. Results: The CTP–amiodarone remained stable for up to 21 days at 37 °C. At ~1/15th of the dose of amiodarone, the CTP–amiodarone decreased the CV in hearts significantly compared to the control GPs (0.92 ± 0.05 vs. 1.00 ± 0.03 ms, p = 0.0007), equivalent to amiodarone alone (0.87 ± 0.08 ms, p = 0.0003). Amiodarone increased the APD (192 ± 5 ms vs. 175 ± 8 ms for vehicle, p = 0.0025), while CTP–amiodarone decreased it significantly (157 ± 16 ms, p = 0.0136), similar to CTP alone (155 ± 13 ms, p = 0.0039). Both amiodarone and CTP–amiodarone significantly decreased the calcium transients compared to the controls. CTP–amiodarone and CTP decreased the CaTD90 to an extent greater than amiodarone alone (p < 0.001). RNA-seq showed that CTP alone increased the expression of DHPR and SERCA2a, while it decreased the expression of the proinflammatory genes, NF-kappa B, TNF-α, IL-1β, and IL-6. Conclusions: Our data suggest that CTP can deliver amiodarone to cardiomyocytes at ~1/15th the total molar dose of the amiodarone needed to produce a comparable slowing of CVs. The ability of CTP to decrease the AP durations and CaTD90 may be related to its increase in the expression of Ca-handling genes, which merits further study.
Adult Mesenchymal Stem Cells from Oral Cavity and Surrounding Areas: Types and Biomedical Applications
Authors: Cabaña-Muñoz María Eugenia , Pelaz Fernández María Jesús , Parmigiani-Cabaña José María , Parmigiani-Izquierdo José María , Merino José Joaquín
Published: August 2023

Adult mesenchymal stem cells are those obtained from the conformation of dental structures (DMSC), such as deciduous and permanent teeth and other surrounding tissues. Background: The self-renewal and differentiation capacities of these adult stem cells allow for great clinical potential. Because DMSC are cells of ectomesenchymal origin, they reveal a high capacity for complete regeneration of dental pulp, periodontal tissue, and other biomedical applications; their differentiation into other types of cells promotes repair in muscle tissue, cardiac, pancreatic, nervous, bone, cartilage, skin, and corneal tissues, among others, with a high predictability of success. Therefore, stem and progenitor cells, with their exosomes of dental origin and surrounding areas in the oral cavity due to their plasticity, are considered a fundamental pillar in medicine and regenerative dentistry. Tissue engineering (MSCs, scaffolds, and bioactive molecules) sustains and induces its multipotent and immunomodulatory effects. It is of vital importance to guarantee the safety and efficacy of the procedures designed for patients, and for this purpose, more clinical trials are needed to increase the efficacy of several pathologies. Conclusion: From a bioethical and transcendental anthropological point of view, the human person as a unique being facilitates better clinical and personalized therapy, given the higher prevalence of dental and chronic systemic diseases.
Metformin Impedes Oxidation of LDL In Vitro
Authors: Rossmann Christine , Ranz Cornelia , Kager Gerd , Ledinski Gerhard , Koestenberger Martin , Wonisch Willibald , Wagner Thomas , Schwaminger Sebastian P. , Di Geronimo Bruno , Hrzenjak Andelko , Hallstöm Seth , Reibnegger Gilbert , Cvirn Gerhard , Paar Margret
Published: August 2023

Metformin is the most commonly prescribed glucose-lowering drug for the treatment of type 2 diabetes. The aim of this study was to investigate whether metformin is capable of impeding the oxidation of LDL, a crucial step in the development of endothelial dysfunction and atherosclerosis. LDL was oxidized by addition of CuCl2 in the presence of increasing concentrations of metformin. The extent of LDL oxidation was assessed by measuring lipid hydroperoxide and malondialdehyde concentrations, relative electrophoretic mobilities, and oxidation-specific immune epitopes. Cytotoxicity of oxLDL in the vascular endothelial cell line EA.hy926 was assessed using the alamarBlue viability test. Quantum chemical calculations were performed to determine free energies of reactions between metformin and radicals typical for lipid oxidation. Metformin concentration-dependently impeded the formation of lipid hydroperoxides, malondialdehyde, and oxidation-specific immune epitopes when oxidation of LDL was initiated by addition of Cu2+. The cytotoxicity of oxLDL was reduced when it was obtained under increasing concentrations of metformin. The quantum chemical calculations revealed that only the reaction of metformin with hydroxyl radicals is exergonic, whereas the reactions with hydroperoxyl radicals or superoxide radical anions are endergonic. Metformin, beside its glucose-lowering effect, might be a suitable agent to impede the development of atherosclerosis and associated CVD. This is due to its capability to impede LDL oxidation, most likely by scavenging hydroxyl radicals.
Research of Transverse Mode Instability in High-Power Bidirectional Output Yb-Doped Fiber Laser Oscillators
Authors: Li Fengchang , Ding Xinyi , Wang Peng , Yang Baolai , Xi Xiaoming , Zhang Hanwei , Wang Xiaolin , Chen Jinbao
Published: August 2023

Bidirectional output fiber laser oscillators can realize two high-power laser outputs employing only a single-laser resonant cavity and hold the advantages of being low cost and of compact size. However, like other fiber lasers, their power improvement is limited by transverse mode instability (TMI). To achieve higher power output, in this paper, the characteristics and corresponding suppression method of the TMI in bidirectional output fiber laser oscillators were investigated for the first time. Firstly, the TMI threshold was obtained when the fiber laser oscillator was pumped by 976 nm LDs and 981 nm LDs, separately, and the difference between the two pumping conditions was researched in detail. After that, a comparison study between the bidirectional and unidirectional output fiber laser oscillators pumped by 981 nm LDs was carried out. In the experiment, the effect of pump distribution on the TMI threshold was also considered. The results show that the TMI threshold of the bidirectional-output laser pumped by 981 nm LDs is much higher than that pump by 976 nm LDs, which means that the effective TMI suppression methods in the unidirectional output laser are also applicable in the bidirectional output laser. In addition, it is found that the TMI threshold of a bidirectional output fiber laser is much lower than that of a unidirectional output fiber laser.