bluuebunny/crossref_metadata_2025 huggingface dataset:
Valuation and Directions of Development of Human Capital in the Republic of Belarus
Authors:   Francisk Skorina Gomel State University, Gevrasyova A. P. , Glukhova I. V. ,   Francisk Skorina Gomel State University, Kazushchik A.A. ,   Francisk Skorina Gomel State University
Published: March 2024

Implementation of the sustainable development model requires a revision of traditional ap-proaches to the concept and valuation of human capital, as its reproduction determines the wealth of a country. Human capital is the basis for the sustainable development of the Republic of Belarus: its preservation and growth affect investments related to the reproduction of capital. Sources of such investments include the net income of the population, funds of organisations and government bodies. The study aims to develop theoretical and methodological foundations for the valuation of human capital it order to determine suitable investment areas. The concepts “human capital” and “investment in human capital” were analysed to identify investment subjects and objects at different levels of the economy. The proposed method for the valuation of human capital relies on the theory of reproduction rent and considers the investment component as a driving factor of its regional and national development. To assess the reproduction of human capital, the study presented a discount indicator as the inverse of the average life expectancy of the population, indirectly expressing its level and quality of life. Data on the socio-economic development of regions of Belarus were examined. Testing of the proposed approach demonstrated that the production of human capital is constant and increases over time. Its sustainable reproduction is affected by the net income of the population and public investments in the social sphere, including education and healthcare. To strengthen human capital, business entities are recommended to invest in improving professional qualifications of employees, as well as their working conditions. The obtained results can be used to develop and implement socio-economic policy at the regional level.
Measurement and Assessment of Human Capital for the Regional Labour Market
Authors:   University of Tyumen, Skipin D. L. , Sapozhnikova A. V. ,   University of Tyumen, Yukhtanova Y. A. ,   University of Tyumen
Published: March 2024

Despite the rapidly growing bibliography and a relative scientific consensus, the measurement of human capital at different levels, degrees of detail, socio-economic traditions remains a topical issue due to the use of various conceptualisation, measurement and interpretation techniques. However, the results of obtaining and measuring human capital so far have fallen short of expectations for a number of reasons, some of which are addressed in this article. Considering the specificity and diversity of Russian regions, assessment of their human capital gained importance in Russian scientific discourse in both quantitative and qualitative research. The present study aims to examine the influence of significant factors, including those determining regional specificity, on the self-assessment of human capital for the labour market in different segments of the economically active population. To obtain data, a new (for Russian scientific discourse) method of asking a group of questions and measuring total human capital for the labour market was applied. The study used the authors’ database and identified groups of factors and their effect on the self-assessment of human capital in accordance with well-known theoretical propositions. As a result, the article explained the influence of both external (satisfaction with living in a region and trust in local authorities) and traditional factors on the quality of human capital for some categories of the working population on the example of a specific Russian region.
Regional Environmental Policy of the Far Eastern Federal District in the Context of National Strategic Goals
Authors:   Institute of Water and Environmental Problems of the Far Eastern Branch of RAS, Mirzekhanova Z. G. , Koltsova A. A. ,   Institute of Water and Environmental Problems of the Far Eastern Branch of RAS
Published: March 2024

In 2020, Russia and its regions adopted new national and regional development programmes, including in the field of environmental protection. Both strategic and tactical issues of environmental policy are addressed at the regional level. The results of previous programmes were disappointing for many reasons. Particularly, national interests are not always considered when developing regional environmental programmes. The present study analyses new environmental programmes adopted in regions of the Far Eastern Federal District and their connection to the federal environmental programme. It is hypothesised that there is a disconnect between the structure and objectives of regional programmes and federal environmental policy. The study analysed environmental safety regulations, government reports and environmental protection programmes of regions of the Far Eastern Federal District. Methods of quantitative and qualitative content analysis, comparative analysis, data visualisation, etc., were utilised. New environmental programmes of Far Eastern regions were reviewed. A conducted analysis of 153 target indicators for achieving the goals of regional programmes indicates a difference between them and the federal environmental programme. Only 50 % of them take into account the federal programme guidelines. An internal content analysis of regional programmes revealed general priorities of the Far Eastern environmental policy: 44 % of the indicators are related to the conservation of terrestrial and aquatic ecosystems. Additionally, 21 % of the indictors relate exclusively to issues of resource industries rather than to environmental protection; for example, 14 % are related to the development of mineral resources. In general, 8 out of 11 programmes take into account the territory’s resource specialisation. Accordingly, recommendations for improving the structure and content of regional programmes were proposed. The results can be used to assess regional environmental policies and create environmental programmes for other territories.
Parameter Identification of the Agent-Based Model for Managing a Regional Industrial Complex
Authors:   Institute of Economics of the Ural Branch of RAS, Akberdina V. V. , Shorikov A. F. ,   Institute of Economics of the Ural Branch of RAS, Korovin G. B. ,   Institute of Economics of the Ural Branch of RAS, Sirotin D. V. ,   Institute of Economics of the Ural Branch of RAS
Published: March 2024

Current challenges facing the Russian economy require models to optimise industrial management processes at the regional level. Therefore, a three-level hierarchical agent-based model for minimax control of the regional industrial complex is considered in this research. The study aims to develop an approach to solving a parameter identification problem of the agent-based model for managing the industrial complex of Sverdlovsk oblast. To this end, the paper presents a theoretical justification for the implemented approach, a formalisation of the aforementioned problem, and an algorithm for constructing and selecting models to assess management system parameters. The method of linear regression analysis was applied to solve the identification problem. The proposed approach was tested using data on 28 types of industrial activity in Sverdlovsk oblast for 2005–2021. The phase vector of statistical identification models was defined by the following parameters: the average annual number of employees of enterprises; fixed assets; gross value added; volume of shipped goods, performed works and services; balanced financial results of enterprises; investment in fixed capital; costs of implementing and using digital technologies. The control vector was determined by the factors of attracting budgetary and other (external) funds. As a result, 125 high-quality models were built to solve parameter identification problems of the agent-based model used for managing the industrial complex of Sverdlovsk oblast. The obtained statistical models can be used to establish communication between agents, clarify their specificity, calculate and assess management performance. The proposed approach can be applied to predict the development of regional industrial complexes in accordance with planned control actions, as well as to calculate control actions necessary to achieve target parameters.
Statistical Analysis of Uneven Digitalization Across Russian Regions and Its Impact on the Total Fertility Rate
Authors:   Ural State University of Economics, Tonkikh N. V. , Kataev V. A. ,   Ural State University of Economics, Kochkina E. M. ,   Ural State University of Economics
Published: March 2024

Russia has been historically characterised by a high regional socio-economic differentiation, including in the sphere of population. Nowadays, information and communication technologies are spreading at different speeds in various regions. Since the impact of digitalisation on fertility is understudied, it is necessary to find methods for identifying connections between them. The paper assesses the development of Russian regions in terms of the total fertility rate (TFR) in regions differently using information and communication technologies. To this end, the study obtained data from the Federal State Statistics Service, namely, from the section “Information and communication technologies” of reports “Regions of Russia: socio-economic indicators”. Univariate and multivariate statistical methods were applied. Russian regions were clustered according to 16 indicators characterising their digital development. Data for 2014 and 2019 were analysed. Three clusters — «best», «average» and «worst» — were identified. The higher polarisation was observed in 2014: 4 regions were included in the “average” cluster, 29 in the “best” cluster, and 46 in the “worst” cluster. In 2019, the polarisation diminished: 45 regions belonged to the “average” cluster, 33 to the “best” cluster, only 4 to the “worst” cluster (Republics of Dagestan, North Ossetia-Alania, Tyva, Chechen Republic). The results show that the total fertility rate is lower in clusters with higher values of digital development. In 2014–2019, TFR decreased by 31.1 % in the “best” and by 47.7 % in the “average” clusters; on the other hand, this indicator increased by 37.7 % in the “worst” cluster. However, it is difficult to assess the exact effect of specific digitalisation factors on fertility due to their complexity and interdependence. Further studies can focus on statistical evaluation of the impact of employment on reproductive behaviour.
Molecular detection of Ovine listeric abortion in Nineveh governorate, Iraq
Authors: Mohammad Hadeel , Ibrahim Al-farwachi Maab , Rasheed Balsam
Published: March 2024

Listeriosis is a important abortive diseases in sheep , and is considered one of the most risky bacterial zoonotic disease worldwide.The study was carried out in 50 sheep flocks placed in the Nineveh governorate, Iraq during November and December 2022, a total of 300 specimens of blood , abomasum content and brain ( 100 each)were collected from local breed ovine aborted fetues(in the last stage of gestation) were obtained to be tested for molecular detection of Listeria monocytogenes . L monocytogenes DNA was detected in a total of 61(20.3%) specimens , distributed as: 35(57.4%) ,15(24.6% and 11(18.0%) strains from fetal brain, abomasum and blood specimens respectively using direct genus-specific conventional polymerase chain reaction (prfA gene) C- PCR. Two L. monocytogenes strains ( HMB1 listeriolysin, HMB 2 listeriolysin ) deposited in GenBank under accession numbers LC769365.1,and LC769366.1. All L. monocytogenes strains were positive for three genes (InlJ, InlA, and hlyA) except actA gene was detected in 46 (75.4%) strains. In conclusion , L. monocytogenes is one of the important causal agents of abortion in sheep flocks in Nineveh province , Iraq, and most of the brain specimens were positive for listeric infection compared to the other specimens.
Autophagosome live cell imaging v1
Authors: Tudorica Dan , not provided bishal.penn
Published: March 2024

For quantification of PINK-PARKIN mitophagy
Revitalization Skywaklk Bandung 2021 Reviving The Urban Area “Urban Space” in Bandung
Authors: Nurcahya Yan , Hadiansyah Tantan , Yosita Lucy , Akbar Adha Syahidil , Allatif I Gilang Miftah , Hilmayani Syalwa Linda
Published: March 2024

Abstract - Skywalk Bandung is well known as a well-known city icon and as a new tourist area in the city of Bandung. Completed and inaugurated 2017 Skywalk Bandung is a mandatory destination for tourists to visit, because it was developed in the Cihampelas area which is an official tourist destination in Bandung. After 2 years of inauguration, the Bandung skywalk has experienced a decline in visitors since its inauguration. Changing the Cipaganti road to one lane, the adjacent Cihampelas road into one lane is the same as Cihampelas making an alternative for tourists to Bandung, changing through the Cihampelas street after traveling to the Lembang area. lane transfer to Jl. Cipaganti being a single lane, helping to deal with congestion and reducing tourists to Jl. Cihampelas, so the construction of the skywalk is less useful than its original purpose. Through the journal that we convey today, it is hoped that it can become a reference method that can be used by the Bandung city government to be able to revive the city facilities that are not functioning properly with the initial plan targeted by the Bandung City Government. Such projects require special attention and, as explained at the beginning, an integrated approach due to the need to manage a large number of urban structures and significant financial investments. The construction of the Skywalk phase II, which is still unfinished, is the main problem that results in the neglect of the initial target development goals of the Skywalk, the neglect of completion of the construction is a major part of the revitalization that must be done.
The Use of Alpha Brainwave to Develop Emphaty Awareness of Vocational School Teachers in Managing Classroom Activities
Authors: Gaffar Muhammad Andriana , Hamdani Hamdani
Published: March 2024

This study aims to explore the benefits of Alpha brainwaves in developing teacher empathy awareness when managing classroom activities on vocational school. The reason for the research being conducted is the fact that most of the teachers are still struggling and find it difficult to deal with students' misbehavior in the classroom because it has an impact on the overall class activities. One of the competencies that every teacher must have is social competence, namely the ability to manage social relations which requires various skills, abilities and capacities in solving problems that occur in interpersonal relationships. The significance of social competence for teachers can be felt in many social contexts. This study uses a grounded theory design with a qualitative approach that is applied to produce a theory that can explain, at a broad conceptual level, processes, actions, or interactions about substantive topics. Data collection techniques used in this study were documentation studies, participatory observation techniques; autoanamnesis interview technique; and a written assessment using a test tool in the form of the Profile of Nonverbal Sensitivity Test (PONS Test). The stages developed to apply Alpha brain waves in increasing teacher awareness of empathy are, 1) Build and Maintain Rapport; 2) Calibration; 3) Map the Brain; 4) Assertiveness Installation; 5) Drop the Anchor; and 6) Provide Feedback. Through these stages, the teacher's brain waves will be adjusted to Alpha waves, and the problems they have to solve are explored. The results of the analysis show that the proper use of Alpha brain waves can provide benefits in increasing teacher empathy awareness when they manage learning activities in the classroom.
THE EFFECT OF STUDENTS SOCIAL INTERACTION ON THEIR LEARNING MOTIVATION DURING DISTANCE LEARNING TO MODELING AND BUILDING INFORMATION DESIGN PROGRAM (MBID) STUDENTS AT PUBLIC VOCATIONAL HIGH SCHOOL (PVHS) IN BANDUNG
Authors: Islami Intan Salamah Nur , Busono Rr Tjahyani , Mardiana Riskha
Published: March 2024

Thesis with the title “The Effect of Students Social Interaction on Their Learning Motivation During Distance Learning to Modeling and Building Information Design Program (MBID) Students at Public Vocational High School (PVHS) in Bandung” is a research during distance learning that observe about students social interaction and their learning motivation in case of MBID program students at PVHS in Bandung. Distance learning performs with online and offline learning that be adapted by government regulations of pandemic learning situation. Its impact the change of social interaction on learning process as online learning from home and offline learning at school with Covid-19 protocol. This change is during distance learning involve students interaction would change with their teachers, their parents, and the other students. It can affect the students learning motivation, to their intrinsic and extrinsic motivation. This thesis is using quantitative method by questionnaire spread with likert scale, it’s analyzed with compare means and simple regression analysis. The result is indicate the students social interaction and their learning motivation in a good category with each means score 3.75 and 3.76, it also points there is effect of students social interaction on their learning motivation with the value until 63.7%.
TRANSFORMING ARCHITECTURAL LEARNING INTO COMMUNITY SERVICE: A REFLECTION ON THE DESIGN OF FISHPONDS AND ISLAMIC BOARDING SCHOOL IN BANONGAN USING ACTOR-NETWORK THEORY
Authors: Fala Akbar , Ekomadyo Agus Suharjono , Prasetyo Eko Bagus
Published: March 2024

According to Tri Dharma all universities in Indonesia have three essential roles comprising education, research, and community service. Every academic community is obliged to carry out and integrate these three roles. However, in reality, the development of science and technology through education and research is still often not integrated into the impactful implementation for community. As a member of academic community, an architecture student can contribute to bridge the gap between the development and the implementation of science and technology, for example by implementing architectural learning to design several social facilities using collective approach. Therefore, this research discussed the transformation of architectural learning into community service by taking a case study on the design of fishponds and Islamic boarding school in Banongan, Situbondo Regency. This qualitative research was conducted to reflect on the transformation process using the perspective of actor-network theory. The results showed that the synergy between architectural learning and community service could provide a learning space to advance pro-community architectural knowledge and produce pro-innovation social facilities for the welfare of community.
KNOWLEDGE BUILDING ON ARCHITECTURAL CHARACTERISTICS OF PERANAKAN CHINESE HOUSE IN STRAITS SETTLEMENTS AND CHINATOWN OF YOGYAKARTA, INDONESIA: KETANDAN, PAJEKSAN, AND KRANGGAN
Authors: Kumala Thalita
Published: March 2024

Chinese residing in various countries can strongly maintain their culture, shown in the form of a distinctive architectural identity. The principles of Chinese architecture certainly experience adaptation to various natural and cultural contexts of countries outside their home country, the four-seasons country, Mainland China. Current situation of Yogyakarta as a fast-growing tourism destination, diverse in cultures and nature is both an opportunity and threat towards Chinese housing established since 1755. In this research, the principles of Chinese architecture in their home countries will be studied, as a major influence of Chinese Peranakan Housing in Yogyakarta in order to understand and later on categorize each housing based on it’s architectural characteristics. Research methods are in stages, the first stage being literature review on typology and principles of Chinese architecture; the second stage is to structure and analyze the architectural characteristic findings; and the third stage is to draw conclusions from the process of interpretation, answering the research questions. The results are in the form of architectural characteristics derived from philosophy, principles, libation ritual, and kingship in early China to 1900s China.
THERMAL INVESTIGATION AT WONOSOBO MARKET, INDONESIA
Authors: Hermawan Hermawan
Published: March 2024

Thermal comfort is one of the factors influencing the success of the building as a place of human activity. Market building in Indonesia plays an essential role in trading activity. The thermal conditions of the Wonosobo market have problems in realizing the thermal comfort of building users. This research aims to investigate the thermal situation of Wonosobo Market, Indonesia. The research method uses quantitative and qualitative methods. Measurements were made using an infrared thermometer. Subjective measures were carried out on 100 respondents. The results show that the Wonosobo Market building has not been able to meet the expectations of building users. The thermal performance of the Wonosobo Market needs to be improved to comfortably accommodate the activities of building users.
A REVIEW OF BIOPHILIC FACTOR FOR HOTEL DESIGN IMPLEMENTATION IN BATAM
Authors: Agustian Victor , Pinassang Jeanny Laurens
Published: March 2024

Batam City has a high potential in the tourism sector and attract tourists for natural tours, shopping tours, culinary tours, sports tours ,etc. In addition, Batam City is a destination for local people outside Batam to find work, because of many manufacturers (local or foreign companies) have established in Batam. The developments triggered a significant increase in population. As the effect of population growth, development in Batam city is also growing. It has a major impact on the reduction of green space. Many buildings have been created only focusing on their strategic location and the beauty of their exterior design. Therefore, the next development in Batam city must be controlled, so that there is a balance with the environment and doesn’t cause the new problem in the future.In other side, young people nowadays, who are in productive age, demand comfort and practical life. Staying in a hotel (for work or vacation) is a new desire for them to adapt to their modern lifestyle. To meet the demands of modern life and to overcome the potential problems that will arise in the future, the need to realize an environmentally friendly hotel is a must. Biophilic is a concept strategy modified by the addition of green plants as a guide to balancing biotic life in the environment. Biophilic design has embodied an auspicious momentum in human’s relationship with nature. The Implementation of a biophilic hotel design is awaited and will encourage the environment by using the same concept in the future
Benefits of Cooking Shows on Youtube for Students of Casinology of Indonesia University of Education During A Pandemic
Authors: Nikmawati Ellis Endang , Imanadi Rula Kaamila , Mahmudaatulsaadh Ai
Published: March 2024

Abstract - Distance learning implemented during the pandemic emphasizes independent learning and utilizes various kinds of learning resources that are around students. One of the learning resources that can be used is Youtube. Youtube can help the lectures of Culinary Education students at the Indonesia University of Education in completing one of their courses, namely Cipta Boga which requires various inspirations in food processing because Youtube has many cooking shows that can be used as a place to find inspiration. The purpose of this study is to find out the benefits of cooking shows on Youtube for students of the Indonesia University of Education's Culinary Education during the pandemic in the Cipta Boga course. The data collection technique was carried out by distributing questionnaires to students of the Indonesian University Culinary Education class of 2018. The population in this study was 45 people and the sample in this study was the entire population of 45 students of the Indonesian University of Education Culinary Education class 2018. The results showed that the intensity of using the internet, Youtube, and cooking shows during the Cipta Boga course was very frequent. Furthermore, the intensity of watching shows on cooking channels and food reviews that have been listed is never. Then, the intended use of the media that has been listed is very high. Next, the benefits in terms of knowledge and skills are very high. While the benefits in terms of attitude are quite high. Keywords: Benefits, Cooking Videos, Youtube, Student
THE EFFECTIVENESS OF AUTISM CHARACTER STIMULATION ROOM AT AUTISM SERVICE CENTRE BATAM THROUGH BEHAVIORAL ARCHITECTURE
Authors: Owen Mikel , Rangkuty Gladies Imanda Utami
Published: March 2024

Autism or commonly referred to as autism spectrum disorder often occurs in children at an early age. Not only that, the symptoms of autism result in the failure of the brain's nervous system and sensory bodies as well as the intellectual decline in individuals who experience it. Sensory malfunctions in the body eventually form 2 characters with different responses to stimuli in a certain environment, namely, hyposensitivity and hypersensitivity. 1 in 500 children in Indonesia is known to have this disorder, so it becomes an issue that is not only developing in the country but has an effect on the world. With a significant increase in the number of autism cases, the need for space will increase. Architects as a profession involved in design play an important role in creating a conducive space for children with autism. However, the space must be adjusted based on the characteristics of autism, so that it can function well for them. This research was conducted by evaluating spaces that have different levels of stimulation in the Batam City Autism Service Center, then developing them. This study also uses a behavioral architectural approach by reviewing the characteristics of people with autism, so that the room can provide comfort for them. The research method used is a qualitative-descriptive form and analyzes the room based on existing criteria. The results of the research are a multifunctional low-stimulus room and a high-stimulus room that can adapt based on variations in activity.
RELATIONSHIP BETWEEN AN-AEROBIC THRESHOLD, LACTIC ACID, AND VO2 MAX. DURING THE GENERAL PREPARATION PERIOD WITH THE LONG DISTANCE RUNNING PERFORMANCE OF SEA GAMES ATHLETES
Authors: Sihite Pretty , Berliana Berliana , Nugraha Eka , Novian Geraldi
Published: March 2024

This study aims to examine the significant relationship between an-aerobic threshold, lactic acid, and VO2 Max during the general preparation period and the long-distance running performance of SEA Games athletes. The method used in this research is a descriptive method which provides an overview of the variables studied and does not provide research treatment to the sample. The research design used is a correlational design by correlating or connecting the variables studied. The sample in this study was 6 athletes from the 2023 Cambodia SEA Games Indonesian National Training Center in long distance running. Instruments in the form of tests carried out at the FPOK UPI Sport Science Laboratory were given once to samples in December 2022 in the form of: a treadmill to measure VO2 max, an anaerobic threshold indicator in the form of a Polar to measure the anaerobic threshold, and a lactate meter to measure lactic acid. Data in the form of long distance running times for each athlete in the Athletics Championship competition held in November-December 2022 in Thailand and the Athletics Invitation and National Selection SEA Games held in January 2023 in Jakarta were collected. Data analysis was carried out using SPSS version 24 software. The results showed that an-aerobic threshold, lactic acid, and VO2 Max during the general preparation period had a significant relationship with the long-distance running performance of SEA Games athletes. Based on the conclusions above, this research provides implications for lactic acid, an-aerobic threshold, and VO2 Max. The periodization period for the general preparation stage is an important thing that long distance running athletes must pay attention to.
QUESTIONING THE OBVIOUS: A RESOURCE FOR THE CREATIVITY DEVELOPMENT OF FIRST YEAR ARCHITECTURAL STUDENTS
Authors: Gallouzi Samia
Published: March 2024

This study explores new perspectives for transferring and promoting creativity in the teaching of “introduction to architectural design”. The rationale bases on the hypothesis that “questioning the obvious” is a resource for developing creative thoughts in general and consequently that of the first-year architecture students in particular. The study adopts the creative questioning as a framework for questioning the obvious within the studio of introduction to architectural design. As a demonstration of that, it presents a teaching approach of introduction to architectural design, which practice “questioning the obvious” from “[Location Redacted for Blind Peer Review].”
Relationship between Mindfulness and Stress Response in High School Students: Mediating Effect of Self-Regulation Ability
Authors: Choi Yoonjung , Lee Mihee , Choi Jeongik
Published: March 2024

Background: This study explored the applicability of mindfulness meditation as an intervention approach in school counseling for managing daily stress in high school students. The study investigated the mediating effect of self-regulation ability on the relationship between mindfulness and stress response.Methods: Data from 244 high school students (119 men and 125 women) were used to examine the mediating effect, controlling for academic variables using Hayes’ (2013) Process Macro Model 4.Results: There was a positive correlation between mindfulness and self-regulation abilities, and mindfulness and self-regulation abilities were negatively correlated with stress response. Moreover, for students who perceived higher academic achievement levels, self-regulation abilities were positively correlated, and stress response were negatively correlated. Results showed a full mediating effect of self-regulation ability on the adverse relationship between mindfulness and stress response. The mediating effects of self-regulation modes were differentially observed in the adverse relationship between mindful consciousness and attitude dimensions of stress response.Conclusions: These findings suggest that when applying mindfulness meditation as a daily stress management strategy for high school students, enhancing their self-regulation ability should be the focus of intervention.
Expediente
Authors: Cadernos Ibero-Americanos de Direito Sanitário
Published: March 2024

Volume 12, number 4, Oct./Dec. 2023ISSN 2358-1824
The Iberoamerican Journal of Health Law is a quarterly open access publication dedicated to the dissemination of scholarly research in health law in iberoamerican region. It has been published by the Program of Health Law/Oswaldo Cruz Foundation since 2012. It is intended for Law and Public Health professors, researchers, and students, as well as lawyers, health professionals, and managers of health systems and services.
EDITORIAL TEAM
Editor-in-chief
Sandra Mara Campos Alves, Health Law Program, Fiocruz Brasilia, Brazil
https://orcid.org/0000-0001-6171-4558
Assistant Editor
Jarbas Ricardo Almeida Cunha, Office of the Public Defender, Brazil
https://orcid.org/0000-0001-5332-2642
Associate Editors
Marcelo Lamy, University of Santa Cecília, Brasil
https://orcid.org/0000-0001-8519-2280
Edith Ramos, Federal University of Maranhão/CEUMA University, Brasil
https://orcid.org/0000-0001-6064-1879
Managing Editor
Glaucia Cristina dos Santos Cruz, Health Law Program, Fiocruz Brasilia, Brazil
https://orcid.org/0000-0001-9511-6548
Editorial Assistant
Danilo Silva Santos Rocha, Health Law Program, Fiocruz Brasilia, Brazil
https://orcid.org/0000-0002-7487-2309

EDITORIAL BOARD
André den Exter, Erasmus University Rotterdam, Erasmus School of Law, Neetherlands
https://orcid.org/0000-0002-0938-6603
André Dias Pereira, University of Coimbra, Portugal
https://orcid.org/0000-0003-4793-3855
Angel Pelayo Gonzáles-Torre, International University of Menéndez Pelayo, Spain
https://orcid.org/0000-0002-6254-9651
Caristina Robaina Aguirre, National Institute of Workers´' Health, Cuba
https://orcid.org/0000-0001-7725-4242
Eli Iola Gurgel Andrade, Federal University of Minas Gerais, Brazil
https://orcid.org/0000-0002-0206-2462
Giancarlo Corsi, Modena e Reggio Emilia University, Italy
https://orcid.org/0000-0003-3259-5734
Hernando Torres Corredor, National University of Colombia, Colombia
Joaquín Cayon de las Cuevas, Cantabria University, Spain
https://orcid.org/0000-0002-1027-9717
Jose Geraldo de Sousa Junior, University of Brasilia, Brazil
https://orcid.org/0000-0002-8974-2283
Márcio Nunes Iorio Aranha Oliveira, University of Brasilia, Brazil
https://orcid.org/0000-0001-9436-0987
Maria Célia Delduque, University of Brasilia, Brazil
https://orcid.org/0000-0002-5351-3534
Miriam Ventura da Silva, Federal University of Rio de Janeiro, Brazil
https://orcid.org/0000-0001-8520-8844
Paula Lobato de Faria, NOVA University of Lisbon, Portugal
https://orcid.org/0000-0002-3290-4480
Vera Lúcia Raposo, University of Coimbra, Portugal
https://orcid.org/0000-0001-7895-2181
Socio-public journalism of father Ostap Nyzhankivskyi: sociocultural dimension
Authors:   Ivan Franko Drohobytsky State Pedagogical University, Molchko Ulyana
Published: March 2024

Newspaper materials on social and educational topics of the priest, composer, conductor, journalist, and social and cultural figure of the end of the 19th – first half of the 20th centuries, Fr. Ostap Nyzhankivskyi, have been analyzed in the article. His journalistic cooperation with the Lviv periodicals "Dilo," "Hospodar I Promyslovets" ("Owner and Industrialist), and "Pidhirska rada" has been considered. The thematic range of newspaper articles, which contain rich, informative material on the history of the birth of the cooperative movement in Eastern Galicia, the functioning of nationally oriented societies, particularly "Sich" and the formation of Ukrainian education, has been highlighted. The genre features of the press publications of the Fr. O. Nyzhankivskyi have been characterized. Based on the conducted scientific research, an analysis of the journalism of a Galician journalist has been carried out for the first time through the prism of socio-cultural processes that took place in Eastern Galicia at the beginning of the 20th century. The research methodology is based on a combination of general scientific and special art historical methods, in particular those from historical and cultural studies. The specified methodological approach allows us to identify and understand a significant number of sources, particularly newspaper materials from the selected magazines, which influenced the formation of the national consciousness of Ukrainian citizens. Analysis of scientific works, which covered the biography and journalistic activity of the social activist Fr. O. Nyzhankivskyi, using a historical and cultural approach, helped determine his place in the socio-cultural space of Eastern Galicia at the beginning of the 20th century. It has been established that the Galician journalist's press publications have an axiological character, which is determined by the social issues highlighted by the publicist. The value of source studies and cognitive and educational functions of socio-cultural press publications of Fr. O. Nyzhankivskyi have been studied. The article will be a reasonable basis for researchers of the role of the personality of the spiritual leader in the nation-building processes of Eastern Galicia at the end of the 19th – the beginning of the 20th centuries, as well as for culturologists and musicologists who study the role of Fr. O. Nyzhankivskyi in sociocultural processes.
Analysis of methods for modeling deep defects of the skin and articular cartilage on laboratory animals in the experiment
Authors: Izbulatov Daulet D. , Varlamova Natalia V. , Mikhailov Vladimir E. , Markin Ilya V. , Erdniev Artur L. , Potapov Petr K. , Utkin Yurii A.
Published: March 2024

The creation and implementation of new methods and means of local treatment of wounds occurs in stages, however, a number of difficulties arise at each stage. The article discusses one of the main problems that arise at the preclinical stage when modeling deep defects of the skin and articular cartilage – the difficulty of accurately reproducing full-layer defects of the skin and articular tissue. Various factors influencing the success of such modeling also investigated, including the type of animal, the size of the defect and its location.
Hemostatic activity in vitro of lectin-containing substances of bryathypes on the example of some Antarctic and Belarus representatives
Authors: Kandelinskaya Olga L. , Grischenko Helena R. , Grigorieva Daria V. , Gorudko Irina V. , Goreckiy Dmitriy V. , Dashkevich Eleonora V. , Peshnyak Janna V. , Bukhvald Natallia A. , Maslovsky Oleg M. , Sysoi Irina P. , Hihiniak Yury G. , Korzun Egor V. , Kostevich Valeria A. , Andreev Mikhail P. , Kurbatova Lyubov E.
Published: March 2024

BACKGROUND: The modern market of medical devices in Belarus and Russia is represented by a wide range of hemostatic agents, of which the most popular are local hemostatics of plant origin possessing the significant technological potential for renewal and improvement. A promising reserve for this may be biologically active compounds of mosses, which are characterized by anti-inflammatory, antibacterial and antifungal effects. However, their hemostatic effect is almost not studied, which determines the relevance of this work.
AIM: The aim of this work is to study the effect of lectin-containing substances from mosses of three species collected in East Antarctica and Belarus on the parameters of human blood hemostasis in vitro.
MATERIALS AND METHODS: We studied mosses of the genera Bryum, Ceratodon, and Coscinodon, collected in the area of the Belarusian Antarctic station Gora Vechernyaya in East Antarctica and in Belarus. Lectin-containing substances of mosses were obtained by extracting shoots in 0.05 M tris-HCl buffer (pH 8.0), centrifugation, filtration. The assessment of the biological activity of lectin-containing substances in mosses was carried out by the agglutination reaction of rabbit erythrocytes, as well as the effect on human platelet aggregation and in the test for activated partial thromboplastin time.
RESULTS: It was established that lectin-containing substances of the studied moss species had agglutinating activity against erythrocytes in the range from 11708.28 (Belarusian samples) to 1333979.59 U/mg of protein (Antarctic samples) depending on the species and localization; initiated the aggregation of human platelets (25–80% of the effect of thrombin) regardless of blood group, Rh and gender of donors; influenced the plasma link of hemostasis, reducing activated partial thromboplastin time (by 15–18%).
CONCLUSIONS: It was found that some species of mosses of the genera Bryum, Ceratodon and Coscinodon of Antarctica and Belarus had an agglutinating and hemostatic effect on erythrocytes and platelets, with the greatest activity noted for Antarctic species. A hypothesis has been put forward that the observed phenomenon is due to the structural features of proteins, including lectins. It is assumed that lectins are possible inducers of erythrocyte agglutination and platelet aggregation in mosses. It is shown that the moss species Bryum pseudotriquetrum and Ceratodon purpureus have a certain resource potential in Belarus for their annual harvest. The results obtained expand the list of moss species with hemostatic activity, and can be used to develop new hemostatics of plant origin for local use from Belarusian plant materials.
Comparative analysis of MxA, OAS1, PKR gene expression levels in leukocytes of patients with influenza and coronavirus infection
Authors: Klotchenko Sergey A. , Romanovskaya-Romanko Ekaterina A. , Oleynik Veronika A. , Egorova Marya A. , Monakhova Varvara S. , Venev Evgeny V. , Plotnikova Marina A.
Published: March 2024

BACKGROUND: The innate immune response, particularly the interferon system, plays a crucial role in defending the host against viral pathogens. Interferon signaling induces the expression of specific antiviral proteins known as interferon-stimulated genes, which inhibit viral replication through various mechanisms.
AIM: This study aimed to develop a quantitative PCR system to assess the molecular regulation of human interferon-stimulated genes MxA, OAS1, and PKR, and to determine their expression in blood leukocytes in response to RNA-containing viruses.
MATERIALS AND METHODS: Leukocytes were isolated from patients with laboratory-confirmed influenza and COVID-19 infections 3–4 days after symptom onset. Ex vivo viral infection was induced using influenza viruses A/California/07/09pdm (H1N1pdm09), B/Malaysia/2506/04 (Vic), strain A2 respiratory syncytial virus, and SARS-CoV-2 HCoV-19/Russia/SPE-RII-3524V/2020.
RESULTS: A multiplex qPCR assay was developed for analyzing human MxA, OAS1, and PKR gene expression, with high amplification efficiency. The test system was used to study the molecular regulation of these genes in leukocytes in influenza and COVID-19 patients. The expression levels of MxA, OAS1, and PKR genes were significantly increased in blood leukocytes of hospitalized patients 3–4 days after symptom onset. Stimulation of leukocytes by influenza A, influenza B, and respiratory syncytial virus led to increased mRNA levels of these genes, while stimulation by SARS-CoV-2 did not result in changes in gene expression.
CONCLUSIONS: The multiplex test system can be used to characterize the expression of antiviral effector interferon-stimulated genes, aiding in the study of virus evasion from the innate immune response.
Changes in DNMT1 expression as a marker of epigenetic regulation disturbanses in multiple sclerosis patients
Authors: Tsymbalova Evgenia A. , Chernyavskaya Ekaterina А. , Ryzhkova Darja Е. , Bisaga Gennady N. , Abdurasulova Irina N. , Lioudyno Viktoria I.
Published: March 2024

BACKGROUND: Multiple sclerosis is a chronic neurodegenerative autoimmune disease characterized by the presence of foci of inflammation and demyelination in the central nervous system. The initiation of pathological processes in multiple sclerosis is caused by a complex interaction of genetic factors, unfavorable environmental factors and epigenetic influences. Progressive neurological symptoms caused by axonal conduction disorders, axonal death and neurodestruction lead to a significant decreased patients’ quality of life and disability. The search for a new markers to improve diagnostic and therapeutic methods, including taking into account the genetic background and epigenetic interactions, is an urgent task.
AIM: The work was aimed to study the changes in DNMT1 mRNA expression in multiple sclerosis patients with different disease duration, to analyze methylation of DNMT1 promoter, and compare the changes in the level of DNMT1 expression with the homocysteine content in the blood, and the presence of polymorphic variants in genes coding the key folate cycle enzymes.
MATERIALS AND METHODS: The level of DNMT1 mRNA expression in peripheral mononuclear blood cells was assessed by reversed transcription followed by polymerase chain reaction. Fluorescent polymerase chain reaction followed by methyl-sensitive analysis of high-resolution melting curves was used to analyze methylation of the DNMT1 promoter. The content of homocysteine in the blood was determined by chemiluminescence immunoassay. The real-time polymerase chain reaction was used for genotyping by polymorphism of folate cycle genes; the fluorescent probes with the LNA modifications were used to discriminate alleles.
RESULTS: It has been shown that in multiple sclerosis patients, including those at the onset of the disease, the level of DNMT1 mRNA expression is significantly lower than in the control group. No relationship was found between the decrease in DNMT1 expression and the level of promoter methylation. Strong positive relationship between the level of DNMT1 mRNA expression and homocysteine content in patients with multiple sclerosis and the combined effects of the genotypes of MTR A2756G and MTHFR C677T polymorphism on the expression of DNMT1 have been shown. These findings suggest that genetically determined features of folate metabolism may contribute to the disruption of epigenetic regulation in multiple sclerosis.
CONCLUSIONS: The obtained results indicate the promise of research aimed to identifying the factors causing epigenetic changes in multiple sclerosis. Studying the mechanisms of the folate cycle genes polymorphic variants contribution to the pathogenesis of multiple sclerosis could be one of the possible ways to improve diagnostic and therapeutic approaches.
Morphofunctional characteristics of the vessels of the small circle of blood circulation in those who died from severe and extremely severe forms of new coronavirus infection
Authors: Emelin Aleksey M. , Sorochanu Irina P. , Asaulenko Zakhar P. , Rogovoy Vasilii А. , Popov Oleg S. , Mosenko Sergei V. , Apalko Svetlana V. , Buchaka Anton S. , Gladchenko Sergey V. , Anisenkova Anna Yu. , Shcherbak Sergey G. , Deev Roman V.
Published: March 2024

BACKGROUND: An important feature of COVID-19 is the development of pronounced hypercoagulation with an increased risk of thrombotic damage to the pulmonary vascular bed, mainly the pulmonary arteries. Thrombosis of the pulmonary blood vessels causes a local violation of hemodynamics with the development of hyperemia, edema, which leads to a decrease in ventilation of the lung tissue area and serves as one of the causes of respiratory failure.
AIM: This study aimed to conduct a morphological and morphometric analysis of the vascular bed of lung tissues in deceased with severe and extremely severe forms of new coronavirus infection who were on inpatient treatment in the period 2020–2022.
MATERIALS AND METHODS: A pathomorphologic study of 129 autopsy cases with a confirmed diagnosis of a new coronavirus infection COVID-19 was performed. Morphometric analysis and statistical data processing of the pulmonary vascular system in histologic preparations stained with hematoxylin and eosin stain, orcein stain and Martius Scarlet Blue (MSB) stain was performed. The control group consisted of 14 patients who died of cardiovascular disease with bilateral focal confluent pneumonia.
RESULTS: It was found that the proportion of thrombosed vessels in the lung tissues of the deceased was 27.6%. In 87.2% of cases, thrombosis develops in small arteries (lumen diameter 30-500 microns) and small veins (lumen diameter 40–500 microns). The vascular-functional indices of Kernogan and Vogenworth were statistically significantly increased in small arteries and small veins of the 4th order (p=0.001), small arteries (p=0.001) and small veins of the 5th order (p=0.014) compared with the control group.
CONCLUSIONS: Diffuse involvement of small caliber blood vessels in the pathological process reflects the severity of specific hemocoagulopathic disorders in the lung tissue. Such disorders lead to the development of ventilation-perfusion disorders and entail an increase in right ventricular failure.