bluuebunny/crossref_metadata_2025 huggingface dataset:
Morfo-anatomia da semente de Bauhinia monandra Kurz: (Leguminosae-caesalpinioideae)
Authors: Ilkiu-Borges Fernanda , Mendonça Maria Sílvia de
Published: January 2010

Bauhinia monandra, espécie popularmente conhecida na Amazônia como pata-de-vaca, é indicada entre outros usos como planta medicinal nessa região. Visando contribuir para um melhor conhecimento foram coletadas sementes dessa espécie do banco de germoplasma da EMBRAPA - CPATU, Amazônia Oriental, no estado do Pará. O estudo morfo-anatômico revelou sementes estenospérmicas, oblongas, base arredondada, margem inteira, ápice assimétrico, perfil convexo pouco acentuado e hilo em forma de "V" na região apical. A testa é monocrômica, brilhante, lisa e constituída por dois estratos epidérmicos; linha lúcida presente. O endosperma é contínuo, espesso, adnato a testa. O embrião é axial e invaginado, apresenta dois cotilédones foliáceos e eixo embrionário com plúmula rudimentar e radícula imperceptível. Foram vistos grãos de amido no interior dos cotilédones.
Status em sociedades pós-modernas: a renovação de um conceito
Authors: Ollivier Michèle
Published: January 2010

Este artigo examina as várias formas com que o termo status tem sido definido na sociologia e identifica algumas das limitações dos conceitos clássicos para a compreensão da desigualdade nas sociedades contemporâneas. Defende que os conceitos neodurkheimianos baseiam-se em pressupostos insustentáveis a respeito do papel integrador do status no contexto atual de relações sociais múltiplas, segmentadas e indiretas. Os conceitos neoweberianos, por sua vez, são úteis para se compreender como avaliações de superioridade são utilizadas por grupos como forma de legitimar a exclusão social, mas são menos úteis para compreender como avaliações coletivas de superioridade e inferioridade resultam na desigualdade entre indivíduos formalmente iguais em ambientes competitivos.
Progresso e mimesis: ideias políticas, imitação e desenvolvimento
Authors: Chisholm Robert
Published: January 2010

A ciência política comparativa vinha ignorando o papel central que ideias e modelos políticos têm na criação e no desenvolvimento das instituições. Com o colapso do bloco soviético e o fervor intelectual que acompanhou esse evento, criou-se uma abertura pela qual os efeitos do pensamento político passaram a ser vistos como importantes. Articuladas por pensadores políticos, as ideias dirigem ações, refletem entendimentos dominantes entre as elites sobre a realidade ou fornecem uma base para a crítica desses entendimentos. Nesse sentido, seu estudo pode revelar alguns aspectos do esforço de constituição de um regime. Este artigo sugere uma forma de abordar o problema: examinar como as ideias são adotadas, adaptadas e assimiladas pelos atores políticos.
A dimensão econômica da teoria política aristotélica
Authors: Rossi Miguel Angel , Tierno Patricio
Published: January 2010

O objetivo do artigo é enfatizar o lugar que ocupa a economia na cosmovisão política de Aristóteles, assumindo o pressuposto segundo o qual o filósofo considera a economia como uma dimensão central da mesma forma que uma condição de possibilidade para pensar a comunidade política. Nesse sentido, percorre-se três aspectos de tal problemática. O primeiro aspecto, o mais visível, cujo descobrimento é mérito da hermenêutica arendtiana, é aquele que diz respeito especificamente ao problema da crematística como desconstrução do objeto da política, considerada como vida comunitária ligada ao bom viver. O segundo aspecto é o que conduz o filósofo a vincular, pela primeira vez na história do Ocidente, os regimes políticos à estrutura social da polis. O terceiro aspecto consiste em realizar uma leitura dos regimes políticos na chave econômica, aspecto central das profundas críticas de Aristóteles à oligarquia. Desse modo, deixa-se explícita outra das hipóteses que se sustenta no texto: a de que as reflexões de Aristóteles se concentram na noção de esfera pública e, por isso, privilegiam teoricamente a aristocracia, a politeia e, inclusive, a democracia.
A propos des enjeux contemporains du structuralisme
Authors: Douville Olivier
Published: January 2010

Comment rendre compte d'une possible actualité du structuralisme? L'auteur s'attache à distinguer un structuralisme qui ne repose que sur des antinomies d'un autre qui suppose le dépassement des antinomies.
Lacan, deleuze e guattari: escritas que se falam
Authors: Palombini Analice de Lima
Published: January 2010

O presente artigo busca estabelecer as balizas para um trabalho de aproximação entre o pensamento de Lacan e o de Deleuze-Guattari, acompanhando as reverberações do que escrevem Deleuze e Guattari no texto lacaniano, e vice-versa, como escritas que se falam. A pesquisa considera que o debate mantido entre esses autores - mesmo quando não explicitado - marcou de forma decisiva as suas produções, dando mostras da efetividade de uma interlocução que, ao pôr em causa suas diferenças, fez-se produtora de movimentos e transformações. Vale-se, nesse sentido, como método de pesquisa, da noção de amizade, tal como formulada por Foucault e Derrida.
O nome que falta
Authors: Sousa Edson Luiz André de , Lima Manoel Ricardo de
Published: January 2010

Este artigo discorre sobre a potência da escrita poética mostrando que o poema vem como um empréstimo ao "nome que falta", uma luta para que este lugar da nomeação continue vazio, porém vivo. Isto não implica evidentemente em uma atitude de mudez. Pelo contrário, ao ousar novos significantes o poeta aciona o fracasso do signo em dizer algo do que é. Procuramos articular um fio de argumentação sobre o compromisso ético e político da poesia a partir das sugestões de imagem de um poema de Joaquim Cardozo (1897-1978) intitulado Canto da Serra dos Órgãos.
Symptôme, sublimation, sexuation: les prolongements du symptôme
Authors: More Geneviève
Published: January 2010

à partir du commentaire de Lacan sur Joyce, j'ai développé une thèse lacanienne fondamentale pour penser la transmission entre les générations avec un autre concept que celui de l'identification : le prolongement du symptôme. De plus, l'accent mis sur le sinthome par Lacan, n'empêche pas de situer la sublimation comme l'un des autres prolongements possibles du symptôme initial de Joyce.
Língua e vida pulsional: o processo de representação da pulsão
Authors: Oliveira Luís Fernando Lofrano de
Published: January 2010

Este artigo visa salientar a participação própria da língua na realização do processo de representação e na determinação da vida pulsional. Com base em conceitos do campo da psicanálise e em obras de S. Freud e J. Lacan, o presente estudo retoma considerações acerca do processo de representação e coloca em discussão a função desempenhada pela língua no seu potencial de simbolização da pulsão. Obtêm-se, com isto, precisões teóricas acerca das condições da realização do processo de representação da pulsão.
Linguagem como alternativa ao aspecto quantitativo em psicanálise
Authors: Beividas Waldir , Ravanello Tiago
Published: January 2010

O presente artigo faz parte de uma pesquisa mais ampla que visa explorar as conseqüências intrínsecas à hipótese do inconsciente estruturado como linguagem se levada à sua radicalidade. Para tanto, focamos a problemática que envolve os conceitos referentes à energética freudiana, especialmente o de afeto, e os eventuais riscos de apropriações por projetos de agregação da psicanálise às neurociências. Ou seja, ao invés de adentrarmos nos meandros do regime quantitativo presente desde a obra freudiana e decorrentes aproximações com temas neurocientíficos, buscamos percorrer a outra possibilidade de abordagem dos fenômenos clínicos e psicopatológicos igualmente latentes no método psicanalítico, a saber, a exploração da linguagem e do sentido.
Letras em oficina: a afirmação retumbante do "não"
Authors: Rickes Simone Moschen , Gleich Paulo
Published: January 2010

Partindo do recorte do percurso de uma oficinante, assídua frequentadora da Oficina de Escrita que se realiza semanalmente no Hospital Psiquiátrico São Pedro de Porto Alegre, o presente artigo interroga, desde a Psicanálise de Freud e Lacan, as relações existentes entre negação, agressividade e simbólico. A elaboração tem como horizonte refletir sobre a produção da perda como um dos operadores do trabalho em oficina junto a sujeitos que encontraram importantes impasses em sua estruturação.
Enterocyte ultrastructural alterations following intestinal obstruction in rats
Authors: Leite Junior Rubens , Mello Natalia Bernardes , Pereira Lara de Paula Miranda , Takiya Cristina Maeda , Oliveira Carlos Alberto Basílio de , Schanaider Alberto
Published: January 2010

PURPOSE: To analyze the small intestinal mucosa ultrastructure, and to characterize the enterocyte lesion severity caused by mechanical intestinal obstruction combined or not with an ischemia of the mesenteric marginal vessels arch. METHODS: It was used 47 Wistar rats divided into 4 groups as follows: Group 1- Control rats (C), Group 2- sham rats (S), Group 3- rats submitted to intestinal obstruction excluding marginal vessel (OEV), Group 4- Intestinal obstruction including marginal vessels (OIV). Rats of groups 3 and 4 were allotted into two subgroups for the removal of small intestinal tissue samples, one at the proximal (P), and the other at the distal (D) segments from the obstruction site. Samples of groups 2, 3, 4 were obtained 24, 48, and 72 hours after post operation care. Small intestinal tissue samples of group 1 were excised following laparotomy. Imaging in Light and Transmission Electronic Microscopy were used for morphological and morphometric studies. The results were analyzed by using the ANOVA and Newman-Keuls tests. RESULTS: No irreversible lesion was observed. In the 24 hours microvilli volume of group 3 turned down at the proximal site henceforth enlarging very slowly within the next 72 hours. At the distal site significant microvilli shrinkage was observed up to 48 hours. Then they recovered their volume after 72 hours. In the 24 hours microvilli volume of group 4 grew twice in comparison with the microvilli of group 1 rats but after 72 hours there was drastic volume shrinkage, shape alterations, and severe flatness, especially in the distal segments of the obstruction site. CONCLUSIONS: Terminal ileum mechanical obstruction with mesenteric marginal arch ischemia led to reversible ultrastructural alterations after 72 hours, and the injury is proportional to the persistence of the obstructive process. Furthermore the mesenteric vessels of the marginal arcade play an important role in the maintenance of mucosal integrity, when such obstructive disorder is present.
Characterization of pseudarthrosis with ultrasound backscattered signals in rats
Authors: Ferreira Manoel Luiz , Matusin Daniel Patterson , Machado Christiano Bittencourt , Silva Paulo César , Mello Natalia Bernades , Amaral Alessandra Cintra , Franco Renata Schueler , Pereira Wagner Coelho de Albuquerque , Schanaider Alberto
Published: January 2010

PURPOSE: To propose a novel model of pseudarthrosis in a small animal and to investigate the ability of backscatter parameters from ultrasound signals in differentiating normal bone from those ones with pseudarthorsis. METHODS: Twelve Rattus norvegicus albinus free from pathogenic species (SPF) were randomly divided in two groups, with six animals each. In the Control group a surgical approach to the femur was made, followed by the synthesis of the muscle and skin layers. The Experimental group was submitted to an osteotomy of the femur and a vascularized flap of the fascia lata was interposed in the line of the fractured bone. Then the alignment and bone stabilization were accomplished, by using nylon stitch in U shape introduced in holes made in the proximal and distal fractured bone. Bone samples were scanned with ultrasound and signals were collected for each one to analyze the parameter Apparent Integrated Backscatter - AIB. RESULTS: Radiological and anatomopathologic studies revealed the absence of bone consolidation with persistence of fiber-osteoid tissue. Values of the ultrasound parameter AIB from normal bones were statistically different from those with pseudarthrosis. CONCLUSION: The experimental model was suitable for pseudarthrosis development in rats and the ultrasound backscatter parameters were able to identify such a bone disease in vitro.
Qualitative and quantitative analysis of rabbit's fat mesenchymal stem cells
Authors: Mazzetti Marcelo Paulo Vaccari , Oliveira Isis Sousa , Miranda-Ferreira Regiane , Fauaz Grasiele , Ribeiro Chaibe Nunes , Gomes Paulo Oliveira , Pontes Paulo , Ferreira Alice Teixeira , Eça Lilian Piñero
Published: January 2010

PURPOSE: To present an experimental model of qualitative and quantitative analysis of mesenchymal stem cells from fat of rabbits obtained by lipectomy. The fat could be a great source for obtaining mesenchymal stem cells and to create conditions for repairing injured tissues by bioengineering. METHODS: New Zealand rabbits (n= 10) adipose panicle (2-3 cm) were removed by lipectomy, fragmented and washed with PBS and enzymatically dissociated with trypsin/EDTA. Lately, these cells were incubated in culture medium DMEM and after 20 days, was performed quantitative analysis of the accession of first and second mesenchymal cells in cell culture bottles. RESULTS: The fat total cells (CTF) were 1.62 x10(6) cells/mL and presented 98% of viability. These cells were taken for cultivation and after 20 days were counted 2.88 x10(6) cells/mL MSC. The same was done and after 20 days we quantified 4.28 x10(6) cells/mL MSC. CONCLUSION: The lipectomy of adipose panicule is a very satisfactory method to extract stem cells from fat, quantitatively and qualitatively.
Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats
Authors: Dantas Anna Carolina Batista , Batista-Júnior Francisco Fábio de Araújo , Macedo Larissa Freitas , Mendes Mariana Noronha Castro , Azevedo Ítalo Medeiros , Medeiros Aldo Cunha
Published: January 2010

PURPOSE: Cyclophosphamide (CYP) is an antineoplastic agent used for the treatment of many neoplastic and inflammatory diseases. Hemorrhagic cystitis is a frequent side effect of CYP. Several studies show that simvastatin has important pleiotropic (anti-inflammatory and immunomodulatory) effects. The purpose of the study was to investigate the effect of simvastatin on bladder, ureter and kidney injury caused by CYP. METHODS: Adult male Wistar rats were randomly divided into three groups. The CYP/SIM group received simvastatin microemulsion by gavage during 7 days (10 mg/kg body wt) before the administration of CYP and the CYP/SAL group rats received saline 0.9%. The control rats were not treated. After that, all rats were treated with a single dose of CYP 200 mg/kg body wt intraperitoneally. The rats were killed 24 h after CYP administration. Plasma cytokines (TNF-α, IL-1β, IL-6) were measured by ELISA. Macro and light microscopic study was performed in the bladder, kidney and ureter. RESULTS: In the bladders of CYP/SIMV treated rats edema of lamina propria with epithelial and sub-epithelial hemorrhage were lower than in CYP/SAL treated rats. The scores for macroscopic and microscopic evaluation of bladder and ureter were significantly lower in CYP/SIMV rats than in CYP/SAL rats. The kidney was not affected. The expression of TNF-α, IL-1β and IL-6 was significatly lower in CF/SINV rats (164.8±22, 44.8±8 and 52.4±13) than in CF/SAL rats (378.5±66, 122.9±26 e 123.6±18), respectively. CONCLUSION: The results of the current study suggest that simvastatin pretreatment attenuated CYP-induced urotelium inflammation and decreased the activities of cytokines.
Metabolic programming of lipid profile and reproductive organs weight by leptin treatment on early life
Authors: Marques Durval Santos , Gombar Flávia Meireles , Pereira Jorge Luiz Alves , Sampaio Francisco José Barcellos , Ramos Cristiane da Fonte
Published: January 2010

PURPOSE: To evaluate whether the neonatal leptin treatment during the first days of life can program the male reproductive organs weight and the lipid profile. METHODS: At birth 6 dams were divided into 2 groups: Leptin - each pup was injected with 50μL of recombinant rat leptin (80ng/g BW, sc), for the first 10 d of lactation; Control - each pup received the same volume of saline. After weaning, all pups received unlimited access to food until 190 days of age when they were killed. Values are given as mean ± SEM of 6 animals and Test t Student was used to analyze the results. RESULTS: The leptin treatment resulted in a significant increase in body weight (Control= 411.8±16.31; Leptin= 481.8±11.29, p=0.005) and food consumption (Control= 25.32±0.09; Leptin= 32.42±0.15, p=0.0001) and a significant reduction in triglycerides levels (Control= 540.0±117.9; Leptin= 93.25±15.21, p=0.006) and in the weight of hypothalamus (Control= 0.234±0.016; Leptin= 0.154±0.015, p=0.007), pituitary (Control= 0.104±0.0120; Leptin= 0.033±0.012, p=0.003), testis (Control= 3.75±0.055; Leptin= 3.19±0.10, p=0.002) and prostate (Control=1.641±0.1389; Leptin= 0.91±0.07, p=0.001). CONCLUSION: Leptin treatment on the first days of life can program the reproductive organs weight and the lipid profile of the progeny.
The influence of methylene blue on the healing of intestinal anastomoses subjected to ischemia and reperfusion in rats
Authors: Miranda Eron Fabio , Greca Fernando Hintz , Noronha Lucia , Kotze Luiz Roberto , Rubin Michel Risnic
Published: January 2010

PURPOSE: To investigate the influence of methylene blue, on the healing of intestinal anastomoses subjected to ischemia and reperfusion in rats. METHODS: Forty-five rats divided into the following three groups were used: control (G1); ischemia without methylene blue (G2); and ischemia with methylene blue (G3). A laparotomy was performed and the cranial mesenteric artery isolated. Whereas the cranial artery was temporarily occluded for 45 minutes in groups G2 and G3, prior to enterotomy and intestinal anastomosis, in group G1 the enterotomy and intestinal anastomosis were performed without prior lesion. Afterwards, 2mL of 0.5% methylene blue were instilled in the peritoneal cavities of the animals in group G3, and 2mL of isotonic saline solution in the peritoneal cavities of the animals in group G2. After the reperfusion, an enterectomy and intestinal anastomosis were performed. After the animals had been sacrificed on the seventh day after the operation, the abdominal cavity was examined by resection of a segment of the intestine containing the anastomosis in order to measure its strength and for histopathological examination. RESULTS: Free fluid or abscesses in the peritoneal cavity were rare. When inflammation was analyzed, the group subjected to ischemia without methylene blue had a higher score for mononuclear cells (p=0.021) and granulation tissue (p=0.044). No significant difference was observed in the density of type I or type III collagens. CONCLUSION: The methylene blue did not show beneficial effect on the healing of intestinal anastomoses subjected to ischemia and reperfusion in rats.
Influence of ingaalp laser (660nm) on the healing of skin wounds in diabetic rats
Authors: Carvalho Paulo de Tarso Camillo de , Silva Iandara Schettert da , Reis Filipe Abdalla dos , Perreira Daniel Martins , Aydos Ricardo Dutra
Published: January 2010

PURPOSE: To determine the influence of low-power laser (660 nm) on the collagen percentage and macrophages in skin wounds in diabetic rats. METHODS: 30 male Wistar rats were used, distributed in two groups: laser treated diabetic (n= 15); untreated diabetic (n = 15). The diabetes was induced by intravenous injection of alloxan into the dorsal vein of the penis, at a rate of 0.1 ml of solution per 100 g of body weight. A wound was made on the back of all the animals. Groups 2 were treated with Aluminium Gallium Indium Phosphide - InGaAlP type diode laser (Photon Laser III DMC®) with a continuous output power of 100 mW and wavelength (?) of 660 nm (4 J/cm²) for 24 s. five animal from each group was sacrificed on the 3rd, 7th and 14th days after wounding. Samples were taken, embedded in paraffin, stained with hematoxylin-eosin, Masson's trichrome, and immunohistochemical macrophage. morphometrically analyzed using the Image Pro Plus 4.5 software. The percentages of collagen fibers and macrophages were determined from the samples from the euthanasia animals. RESULTS: The data were treated statistically using analysis of variance (ANOVA) and the Post-hocTukey test. The significance level was set at 0.05 or 5%. CONCLUSION: The low-power laser (660 nm) was shown to be capable of influencing the collagen percentage in skin wounds by increasing the mean quantity of collagen fibers and macrophages.
Obesity and abdominal wound healing in rats
Authors: Biondo-Simões Maria de Lourdes Pessole , Zammar Guilherme Roberto , Fernandes Rodrigo dos Santos , Biondo-Simões Rachel , Mello Flavia Stica Ritzdorf de , Noronha Lucia de
Published: January 2010

INTRODUCTION: Treatment for obesity essentially has to do with weight loss, which can be achieved through surgical procedures. Despite the considerable rise in the number of such procedures, the relationship between obesity and the healing process has not been totally clarified. PURPOSE: To investigate abdominal wound healing in obese Wistar rats on the seventh and fourteenth days following a laparotomy. METHODS: Thirty-six Wistar rats were randomly distributed into two groups, the control and experiment group. The control group were fed on either a standard diet for the species and the experiment group were put on a high calorie diet. After 116 days, all the animals were submitted to a laparotomy followed by laparorrhaphy. After euthanasia on the seventh or fourteenth day, fragments of the abdominal wound containing the scar were submitted to histopathological and tensiometric analysis. RESULTS: The average weight of the animals from the experiment group was higher than that of the control group (p<0.001). The difference in the resistance of the cutaneous scars was not significant. The aponeurotic scars were more resistant in the control group after seven days (p=0.011) and fourteen days (p=0.040). There was no difference in terms of intensity of the inflammatory reaction and the collagen density was similar in both groups. CONCLUSION: In rats, obesity lowered the resistance of the aponeurotic scars but not the skin scars. It did not interfere with the delayed inflammatory response and the collagen density.
Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress
Authors: Gragnani Alfredo , Rossi Marina Bertelli , Albuquerque Janne Cely Medeiros , Brito Gabriela Soares Silva , Aloise Antonio Carlos , Ferreira Lydia Masako
Published: January 2010

PURPOSE: To evaluate effects of oxidative stress and supplementation of keratinocyte growth factor (KGF) on cultivated human keratinocytes. METHODS: Oxidative stress was produced through addition of hydrogen peroxide (H2O2) to the culture medium. Cultivated human keratinocytes were divided in 4 groups: Group control (G C), Group KGF (G KGF), Group H2O2 (G H2O2), Group H2O2 and KGF (G H2O2-KGF). Each experiment was accomplished with the same lineage cultivated keratinocytes, in triplicate. Cell viability was evaluated by trypan blue exclusion assay. RESULTS: The results showed that the culture medium supplemented with KGF presented a small rate of cell viability when compared to cells only in culture medium (p<0,001). It demonstrated that only the growth factor does not have protector effects for cells in vitro. However, in front of the oxidative stress produced by addition of hydrogen peroxide to the medium, KGF showed a beneficial effect, protecting cells when compared to the group that suffered hydrogen peroxide action but had not been exposed to KGF (p<0,001). CONCLUSION: KGF determined protection to the primary human keratinocytes exposed to oxidative stress.
Action of tacrolimus on Wistar rat kidneys implanted with Walker 256 carcinosarcoma
Authors: Inácio Cristiano Machado , Dietz Ulrich Andréas , Malafaia Osvaldo , Ribas Filho Jurandir Marcondes , Nassif Paulo Afonso Nunes , Czeczko Nicolau Gregori , Marcondes Carmen Australia Paredes
Published: January 2010

PURPOSE: To evaluate the development of Walker 256 tumor in male Wistar rats treated with tacrolimus using an experimental kidney tumor model. METHODS: 40 male Wistar rats were divided into four groups: Tumor group (TU) (n=10), Tacrolimus-Tumor group (TT) (n=10), Tacrolimus group (TC) (n=10) and Control group (C) (n=10). Treatment with tacrolimus was performed in groups TT and TC. Under anesthesia, the right kidney of each animal of TU and TT was accessed through a supraumbilical incision and inoculated with a 0.1mL solution containing 2x10(6) tumor cells (Walker 256 carcinosarcoma tumor cells). Group TC was treated with a saline solution. All the animals of groups TC and TT were treated with tacrolimus (5mg/kg/day) by gavage for 15 days. TU group animals received saline by gavage for 15 days. On the 15th postoperative day, all animals were submitted to euthanasia and blood sampling for analysis of serum creatinine (Cr) and blood urea nitrogen (BUN). Abdominal gross examination was performed, the right kidney removed and prepared for histological analysis by hematoxylin-eosin staining. The resulting data were submitted to statistical analysis by ANOVA. RESULTS: Statistical significance was found when comparing creatinine level between groups TU, TT and TC -TT group culminated with a marked increased in creatinine levels (Cr=1.013 ± 0.3028 mg/mL), TU group (Cr=0.5670 ± 0.03536 mg/dL) P=0.00256, TC group (Cr =0.711 ± 0.1653 mg/mL) P= 0.02832. Statistical significance was found when comparing BUN levels in TT group (71.32 ± 17.14 mg/mL), compared with TU group (45.83 ± 5.046 mg/dL), P=0.000318. There were no statistically significant differences between groups TT and TC (61.23 ± 9.503 mg/mL) P=0.7242. Histological analysis showed a poor evolution in TT group with multiple foci of hemorrhage and cortical invasion by the Walker tumor. CONCLUSION: The Tacrolimus-treated group developed a more aggressive tumor and a drug-related nephrotoxic effect.
Venous drainage from the tail of the pancreas to the lienal vein and its relationship with the distal splenorenal shunt selectivity
Authors: Piras Cláudio , Paulo Danilo Nagib Salomão , Paulo Isabel Cristina Andreatta Lemos , Rodrigues Hildegardo , Silva Alcino Lázaro da
Published: January 2010

PURPOSE: To identify the veins draining from the pancreatic tail to the lienal vein and its possible relationship with the loss of the distal splenorenal shunt selectivity. METHODS: Thirty eight human blocks including stomach, duodenum, spleen, colon and pancreas, removed from fresh corpses, were studied with the replenish and corrosion technique, using vinilic resin and posterior corrosion of the organic tissue with commercial hydrochloric acid, in order to study the lienal vein and its tributaries. RESULTS: The number of veins flowing directly to the splenic vein varied from seven to twenty two (14.52 ± 3.53). Pancreatic branches of the pancreatic tail flowing to the segmentary veins of the spleen were found in 25 of the anatomical pieces studied (65.79%). These branches varied from one to four, predominating one branch (60%) and two branches (24%). CONCLUSIONS: In 65.79% of the anatomical pieces studied, the veins of the pancreatic tail flowed in segmentary branches of the splenic vein. These branches could be responsible for the loss of distal splenorenal shunt selectivity. The complete disconnection of the pancreatic tail could increase the selectivity in this procedure.
Lung apoptosis after intra-pulmonary instillation of Benzo(a)pyrene in Wistar rats
Authors: Silva Baldomero Antonio Kato da , Aydos Ricardo Dutra , Silva Iandara Schettert , Pereira Daniel Martins , Carvalho Paulo de Tarso Camillo de , Dourado Doroty Mesquita , Reis Filipe Abdalla dos , Nacer Renato Silva
Published: January 2010

PURPOSE: To evaluate the influence of pulmonary instillation of Benzo[a]pyrene in lung apoptosis of Wistar rats. METHODS: Male Rattus norvegicus albinus, Wistar lineage was carried through an intra-pulmonary instillation of the Benzo[a]pyrene (B[a]P) dilution in alcohol 70%. Three experimental groups had been formed with 08 animals each: Control Group (Alcohol 70%); B[a]P Group 40 mg/kg; e B[a]P Group 80mg/kg, submitted to euthanasia 16 and 18 weeks after the experimental procedure. The pulmonary sections had been processed by TUNEL method and submitted to the histomorphometric analysis to quantify the apoptotic cell number. RESULTS: After 16 weeks, mean of apoptotic cells number in control group (19,3±3,2) was greater than 40mg/Kg group (11,8±1,9; p<0,01) and 80mg/Kg group (7,0±1,4; p<0,01). Significant difference also observed between 40mg/Kg and 80mg/Kg (p<0,05). After 18 weeks, mean of apoptotic cells number in control group (18,0±2,2) was greater than 40mg/Kg group (8,8±1,7; p<0,01) and 80mg/Kg group (5,5±1,3; p<0,01). Significant difference wasn't observed between 40mg/Kg and 80mg/Kg (ns). CONCLUSION: Intra-pulmonary instillation of Benzo[a]pyrene induces significant decrease of apoptotic activity in lung tissue.
Cardiocirculatory changes in hemorrhagic shock induced in pigs submitted to three distinct therapeutic methods
Authors: Abreu Gilberto Fernandes Silva de , Coelho Antônio Roberto Barros , Aguiar José Lamartine de Andrade , Moura Filho Sílvio Amorim
Published: January 2010

PURPOSE: To evaluate and compare the response of pigs submitted to hemorrhagic shock and treated using three different strategies. METHODS: Thirty-five Dalland pigs were divided into four groups: Control; Bleeding; Saline and Saline + Red Cell Concentrate. Parameters evaluated: heart rate (HR), mean arterial blood pressure (MAP) and central vein pressure (CVP).Hemorrhagic shock was induced by removing (624.25±64.55), (619.30±44.94) and (664.23±39.96) ml of blood respectively, with the following treatment: Bleeding Group - zero volume replacement; Saline Group - replacement with 676 ml of 0.9% saline solution; Saline + Red Cell Concentrate Group - replacement with 440 ml of 0.9% saline solution + 291 ml of red cell concentrate. The treatment was evaluated after 10 (T3), 30 (T4), 45 (T5) and 60 (T6) minutes. RESULTS: HR: No statistically significant difference was found between the Bleeding and Saline [p=1.000], Bleeding and Saline + Red Cell Concentrate [p=1.000], and Saline and Saline + Red Cell Concentrate [p=0.721] groups. MAP; Significant differences were found between all the groups studied. CVP: No significant difference was found between the groups. CONCLUSION: Non-replacement and euvolemic resuscitation maintained a satisfactory hemodynamic pattern in controlled severe hemorrhagic shock in swine. The euvolemic replacement strategies exceeded the limit values of MAP for rebleeding.
Amplificador classe d para subwoofer
Authors: Silva Fabio V. R. da , Martins Leandro dos S. , Vieira Júnior João Batista , Coelho Ernane A. A. , Freitas Luiz Carlos de
Published: January 2010

Este artigo apresenta uma topologia de amplificador Classe D de potência adequado para operar com sub-woofer. A estrutura proposta não necessita de fonte de alimentação estabilizada e filtro passa baixas de saída como ocorre com os amplificadores Classe D tradicionais. Um protótipo de 1 kW foi implementado em laboratório obtendo-se rendimento superior a 90 % e THD inferior a 1 %. Roteiro de projeto, princípio de operação e estratégia de controle também são apresentados.
Controle de máquinas de indução pentafásicas com otimização do campo no entreferro
Authors: Pereira Luís A. , Scharlau César C. , Pereira Luís F. A. , Haffner Sérgio
Published: January 2010

Apresenta-se neste artigo a formulação matemática de um modelo dinâmico para máquinas de indução pentafásicas. O modelo apresentado é baseado em componentes simétricas de valor instantâneo, resultando no desacoplamento das componentes fundamental e de terceiro harmônico da indução no entreferro A partir deste modelo obtém-se dois conjuntos de equações que correspondem a representação em variáveis de estados do efeito de cada um dos componentes harmônicos nas equações da máquina. Resultados de simulação baseados em uma técnica de controle do tipo V/f, a qual utiliza fatores de ponderação para as componentes harmônicas a fim de impor de uma forma de onda de indução trapezoidal no entreferro da máquina, são comprovados experimentalmente validando o modelo proposto.
Investigação crítica do desempenho do GMRES pré-condicionado via fatoração incompleta LU em estudos de fluxo de carga
Authors: Pessanha José E. O. , Portugal Carlos , Saavedra Osvaldo
Published: January 2010

Este artigo investiga causas associadas ao baixo desempenho de um pré-condicionador ILU e propõe uma metodologia para melhorar a sua qualidade e a taxa de convergência do método iterativo GMRES. O objetivo é solucionar o subproblema linear do fluxo de carga, com destaque para condições de carga pesada e de difícil convergência. A metodologia está baseada em duas etapas. Na primeira, um pré-condicionador é construído a partir da matriz Jacobiana da primeira iteração Newton-Raphson reordenada. A segunda etapa só é considerada se o GMRES falhar no início do processo de solução devido à baixa qualidade do pré-condicionamento. Nela, o processo é reiniciado e um pré-condicionador de melhor qualidade é construído a partir da matriz Jacobiana pré-processada via escalonamento e permutações não-simétricas e simétricas (reordenamento). A fim de amenizar o custo computacional associado a sua construção, uma estratégia para manter o pré-condicionador fixo sempre que possível é incluída. Experimentos numéricos corroboram a robustez numérica e a eficiência computacional da metodologia proposta.
Eliciação e comunicação de requisitos em domínios disjuntos: estudo de caso para automação na área médica
Authors: Queiroz Ricardo Alexandro de A. , Silva José Reinaldo
Published: January 2010

O final do século passado foi marcado por um crescente intresse nas fases preliminares do processo de design, especificamente na fase de requisitos e definição do problema. Na área de automação, o gerenciamento de requisitos é determinante para o sucesso dos projetos, embora grande parte do esforço no desenvolvimento de técnicas e métodos ter sido direcionado para as fases posteriores: o design e a implementação. Por não serem formais, os processos de eliciação e análise de requisitos são subestimados por profissionais da área de automação, onde o formalismo é fundamental para a validação dos processos de controle, automação e supervisão. Em especial, quando os ambientes de desenvolvimeno e de aplicação são disjuntos, isto é, usuários e stakeholders pertencem a um domínio com pouca ou nenhuma interseção com a área da engenharia, informática e automação, os processos de eliciação e análise se tornam bastante complicados. Neste trabalho é apresentada uma proposta de sistematização do gerenciamento de requisitos para domínios disjuntos, adaptando técnicas já conhecidas ao processo de prototipagem virtual, consolidado após o início deste século. Um estudo de caso é apresentado onde o desenvolvimento se dá na área de Engenharia de Automação para aplicações em processos pertinentes à cirurgia de catarata. Para aproximar os domínios busca-se uma abordagem mais simplificada das técnicas de prototipagem virtual tornando o processo mais prático, sem no entanto abdicar do formalismo dos modelos. Os dados levantados foram obtidos com a colaboração do Hospital Santa Luzia, Salvador, Bahia, e do Instituto da Visão, UNIFESP, em São Paulo.
Cone-beam computed tomography analysis of the apical third of curved roots after mechanical preparation with different automated systems
Authors: Oliveira Cesar Augusto Pereira , Meurer Maria Inês , Pascoalato Cristina , Silva Silvio Rocha Correa
Published: January 2010

The present study evaluated by cone-beam computed tomography (CBCT) the apical canal transportation and centralizing ability of different automated systems after root canal preparation. The mesiobuccal canals of maxillary first molars (n=10 per group) were prepared with: GI - reciprocating system with K-Flexofile; GII - reciprocating system with NiTiFlex files; GIII - rotary system with K3 instruments; GIV - rotary system with RaCe instruments. CBCT scans were taken before and after biomechanical preparation up to a #40.02 diameter. Canal transportation was determined by measuring the smallest distance between the inner canal walls and the mesial and distal sides of the root. The centralization ability corresponded to the difference between the measurements from transportation evaluation, using the linear voxel to voxel method of analysis. The mean transportation was 0.06 ± 0.14 mm, with a tendency to deviate to the mesial side of the root (n=22), with no statistically significant difference among the groups (p=0.4153). The mean centralization index was 0.15 ± 0.65 also without statistically significant difference among the groups (p=0.0881). It may be concluded that apical canal transportation and centralization ability were not influenced by the type of mechanical movement and instruments used.
Periapical repair after root canal filling with different root canal sealers
Authors: Tanomaru-Filho Mário , Tanomaru Juliane Maria Guerreiro , Leonardo Mario Roberto , Silva Lea Assed Bezerra da
Published: January 2010

The aim of this study was to evaluate periapical repair after root canal filling with different endodontic sealers. Sixty-four root canals from dog´s teeth were filled, divided into 4 groups (n=16). Root canals were instrumented with K-type files and irrigated with 1% sodium hypochlorite solution. Root canals were filled in the same session by active lateral condensation of the cones and sealers: Intrafill, AH Plus, Roeko Seal and Resilon/Epiphany System. After 90 days, the animals were euthanized and the tissues to be evaluated were processed and stained with hematoxylin and eosin. For histopathological analysis, the following parameters were evaluated: inflammatory process, mineralized tissue resorption, and apical mineralized tissue deposition. Histopathological analysis demonstrated that Intrafill had less favorable results in terms of apical and periapical repair, compared to the other sealers (p<0.05). AH Plus, Roeko Seal, and Epiphany sealers had similar and satisfactory results (p>0.05). In conclusion, AH Plus and the materials Roeko Seal and Epiphany are good options for clinical use in Endodontics.
In vitro assessment of solvent evaporation from commercial adhesive systems compared to experimental systems
Authors: Nihi Fabio Mitugui , Fabre Hebert Samuel Carafa , Garcia Georges , Fernandes Karen Barros Parron , Ferreira Flaviana Bombarda de Andrade , Wang Linda
Published: January 2010

Solvents should be properly evaporated after application to dental substrates. The aim of this study was to assess the evaporation of commercial, experimental and neat solvents. The tested null hypotheses were that there are no differences in solvent evaporation regardless of its formulation and over time. Evaporation from commercial adhesive systems (Scotchbond Multipurpose Primer, Scotchbond Multipurpose Adhesive, Prime & Bond NT, Multi Bond, Excite, Single Bond 2, Adhese Primer, Adhese Bond, Xeno III A and Xeno III B) and experimental primers (35% HEMA plus 65% acetone or ethanol or water v/v) were compared to neat solvents (acetone, ethanol and water). Samples (10 µL) of these products were dripped into glass containers placed on a digital precision balance. Evaporation was assessed at 0, 5, 10, 15, 30, 60, 120, 300 and 600 s times to calculate mass loss. Data were analyzed statistically by ANOVA and Bonferroni's correction (a=0.05). Acetone-based products exhibited a remarkable capacity to evaporate spontaneously over time. Neat acetone evaporated significantly more than the HEMA-mixtures and the commercial formulations (p<0.05). The incorporation of monomers and other ingredients in the commercial formulations seem to reduce the evaporation capacity. Solvent evaporation was time and material-dependent.
Effect of laser welding on the titanium composite tensile bond strength
Authors: Galo Rodrigo , Ribeiro Ricardo Faria , Rodrigues Renata Cristina Silveira , Pagnano Valéria de Oliveira , Mattos Maria da Glória Chiarello de
Published: January 2010

The aim of this study was to analyze the shear bond strength between commercially pure titanium, with and without laser welding, after airbone-particle abrasion (Al2O3) and 2 indirect composites. Sixty-four specimens were cast and divided into 2 groups with and without laser welding. Each group was divided in 4 subgroups, related to Al2O3 grain size: A - 250 µm; B - 180 µm; C- 110 µm; and D - 50 µm. Composite rings were formed around the rods and light polymerized using UniXS unit. Specimens were invested and their shear bond strength at failure was measured with a universal testing machine at a crosshead speed of 2.0 mm/min. Statistical analysis was carried out with ANOVA and Tukey's test (α=0.05). The highest bond strength means were recorded in 250 µm group without laser welding. The lowest shear bond strength means were recorded in 50 µm group with laser welding. Statistically significant differences (p<0.05) were found between all groups. In conclusion, airborne particle abrasion yielded significantly lower bond strength as the Al2O3 particle size decreased. Shear bond strength decreased in the laser welded specimens.
Effect of light-curing units, post-cured time and shade of resin cement on knoop hardness
Authors: Reges Rogério Vieira , Costa Ana Rosa , Correr Américo Bortolazzo , Piva Evandro , Puppin-Rontani Regina Maria , Sinhoreti Mário Alexandre Coelho , Correr-Sobrinho Lourenço
Published: January 2010

The aim of this study was to evaluate the Knoop hardness after 15 min and 24 h of different shades of a dual-cured resin-based cement after indirect photoactivation (ceramic restoration) with 2 light-curing units (LCUs). The resin cement Variolink II (Ivoclar Vivadent) shade XL, A2, A3 and opaque were mixed with the catalyst paste and inserted into a black Teflon mold (5 mm diameter x 1 mm high). A transparent strip was placed over the mold and a ceramic disc (Duceram Plus, shade A3) was positioned over the resin cement. Light-activation was performed through the ceramic for 40 s using quartz-tungsten-halogen (QTH) (XL 2500; 3M ESPE) or light-emitting diode (LED) (Ultrablue Is, DMC) LCUs with power density of 615 and 610 mW/cm2, respectively. The Koop hardness was measured using a microhardness tester HMV 2 (Shimadzu) after 15 min or 24 h. Four indentations were made in each specimen. Data were subjected to ANOVA and Tukey's test (a=0.05). The QTH LCU provided significantly higher (p<0.05) KHN values than the LED LCU. When the post-cure times were compared for the same shade, QTH and LED at 24 h provided significantly higher (p<0.05) KHN values than at 15 min. It may be concluded that the Knoop hardness was generally dependent on the LCU and post-cure time. The opaque shade of the resin cement showed lower Knoop hardness than the other shades for both LCUs and post-cure times.
Diagnosis and root canal treatment in a mandibular premolar with three canals
Authors: Soares Lanuce Rosa , Arruda Marcos , Arruda Marcos Pôrto de , Rangel Andréa Leão , Takano Edson , Carvalho Júnior Jacy Ribeiro de , Saquy Paulo Cesar
Published: January 2010

This paper presents a case report of a left mandibular second premolar with three canals and three different apical foramina. A 39-year-old male patient presented to our clinic with pain in the mandibular left second premolar. Initially, pain was caused by cold stimulus and later was spontaneously. The intraoral clinical examination revealed a fractured amalgam restoration with occlusal caries. Percussion and cold (Endo-Frost) tests were positive. The radiographic examination showed the presence of two roots. The probable diagnosis was an acute pulpitis. After access cavity, it was observed remaining roof of the pulp chamber and mild bleeding in the tooth lingual area, indicating the possible presence of a third canal. The endodontic treatment was completed in a single session using Root ZX apex locator and K3 NiTi rotary system with surgical diameter corresponding to a .02/45 file in the three canals and irrigation with 1% sodium hypochlorite. The canals were obtured with gutta-percha cones and Sealer 26 using the lateral condensation technique. After 1 year of follow-up, the tooth was asymptomatic and periapical repair was observed radiographically. Internal alterations should be considered during the endodontic treatment of mandibular second premolars. The correct diagnosis of these alterations by the analysis of preoperative radiographs can help the location of two or more canals, thereby avoiding root therapy failure.
Oral findings and dental care in a patient with myelomeningocele: case report of a 3-year-old child
Authors: Queiroz Alexandra Mussolino de , Saiani Regina Aparecida Segatto , Rossi Cristhiane Ristum Bagatin , Gomes-Silva Jaciara Miranda , Nelson-Filho Paulo
Published: January 2010

Myelomeningocele (MMC) is a congenital malformation of the neural tube that occurs in the first weeks of pregnancy. This malformation refers to the caudal non-closure of the neural tube and neural tissue exposure, which lead to neurological problems, such as hydrocephalus, motor disability, genitourinary tract and skeletal abnormalities and mental retardation. Patients with MMC have an acknowledged predisposition to latex allergy and are usually at a high caries risk and activity due to poor oral hygiene, fermentable carbon hydrate-rich diet and prolonged use of sugar-containing medications. This paper addresses the common oral findings in pediatric patients with MMC, discusses the strategies and precautions to deal with these individuals and reports the dental care to a young child diagnosed with this condition.
Perfil eletroforético de lombo suíno adicionado de proteínas não cárneas
Authors: Daguer Heitor , Stephan Marilia Penteado , Bersot Luciano dos Santos
Published: January 2010

A legislação brasileira permite a adição intencional de proteínas não cárneas em alguns produtos cárneos; entretanto, há poucas técnicas adaptadas e próprias para controle desses ingredientes. Na presente pesquisa, empregou-se a técnica de eletroforese em gel de poliacrilamida na presença de dodecil-sulfato de sódio (SDS-PAGE) para comparar amostras de carne suína (lombo), adicionadas de 1,5% de proteínas não cárneas (proteína isolada de soja e concentrado protéico de soro de leite). Mediante a padronização de dois protocolos distintos de extração (uréia 6M e Tris-HCl-SDS-mercaptoetanol) e a utilização de controles positivos e negativos, obtiveram-se perfis eletroforéticos típicos do músculo e das proteínas utilizadas. Ambas as extrações utilizadas foram adequadas para separação das proteínas do soro de leite das proteínas da carne, mas a visualização dos marcadores da soja não foi possível em razão da sobreposição de bandas.
Avaliação histológica dos testículos de ovinos da raça Santa Inês nascidos em diferentes estações do ano
Authors: McManus Concepta , Sasaki Luiz Claudio Bastos , Louvandini Helder , Dias Laila Talarico , Teixeira Rodrigo de Almeida , Alves Jordan Menezes , Lucci Carolina Madeira , Marsiaj Pablo Henrique Pereira , Murata Luci Sayori
Published: January 2010

Foram analisados quatro grupos de ovinos machos da raça Santa Inês nascidos em quatro estações do ano, no Distrito Federal, a fim de observar as idades e o peso corporal em que estes atingiram a puberdade. A partir dos cortes histológicos dos testículos, foram analisados parâmetros celulares e mensurações dos túbulos seminíferos. O efeito da data de nascimento sobre os parâmetros avaliados foi significativo (P<0,01), indicando que a estação do ano influenciou as características histológicas dos testículos dos cordeiros Santa Inês. Os animais nascidos no período da seca apresentaram maior precocidade à puberdade, porém pior perfil histológico dos testículos. O final da época de águas apresentou-se como a melhor estação para nascimentos de ovinos na região Central do Brasil, com características histológicas testiculares mais desejáveis na puberdade desses animais.