bluuebunny/crossref_metadata_2025 huggingface dataset:
A New Potential Dietary Approach to Supply Micronutrients to Physically Active People through Consumption of Biofortified Vegetables
Authors: Baldassano Sara , Polizzi Maria Rita , Sabatino Leo , Caldarella Rosalia , Macaluso Andrea , Alongi Angelina , Caldara Gaetano Felice , Ferrantelli Vincenzo , Vasto Sonya
Published: July 2022

Micronutrients are required in many reactions involved in physical activity and exercise. Most physically active people do not meet the body’s needs in terms of micronutrients through diet. The novelty of the present manuscript is the use of an innovative dietary approach to supply micronutrients to physically active people through biofortified food. Therefore, the key point of this study was to verify whether supplementation with biofortified vegetables—and specifically molybdenum (Mo)-enriched lettuce—in healthy volunteers affects essential regulators of body homeostasis and, specifically, hematological parameters, iron and lipid metabolism, and hepatic function. Twenty-four healthy volunteers were allocated in a double-blinded manner to either a control group that consumed lettuce, or the intervention group, which consumed Mo-enriched lettuce, for 12 days. Blood samples were collected at baseline (T0) and after 12 days (T1). We found that supplementation with Mo-enriched lettuce did not affect hematological parameters, liver function, or lipid metabolism, but significantly improved iron homeostasis by increasing non-binding hemoglobin iron by about 37% and transferrin saturation by about 42%, while proteins of iron metabolism (e.g., transferrin, ferritin, ceruloplasmin) were not affected. The serum molybdenum concentration increased by about 42%. In conclusion, this study shows that consumption of Mo-biofortified lettuce ameliorates iron homeostasis in healthy subjects, and suggests that it could be used as a new nutritional supplementation strategy to avoid iron deficiency in physically active people.
Regulation of Dietary Protein Solubility Improves Ruminal Nitrogen Metabolism In Vitro: Role of Bacteria–Protozoa Interactions
Authors: Zhang Zhenbin , Wei Wenjun , Yang Sihan , Huang Zeliang , Li Chuang , Yu Xiang , Qi Ruxin , Liu Wujun , Loor Juan J. , Wang Mengzhi , Zhang Xin
Published: July 2022

Precision dietary interventions (e.g., altering proportions of dietary protein fractions) has significant implications for the efficiency of nutrient use in ruminants, as well as lowering their environmental footprint, specifically nitrogen (N) emissions. Soluble protein (SP) is defined as the protein fraction that is rapidly degraded in the rumen (e.g., non-protein N and true protein), and our previous study found that regulating SP levels could improve N efficiency in Hu sheep. Thus, the present study was conducted to explore in vitro how protein fractions with different SP levels modulate the rumen microbial community and its association with N metabolism. Four dietary treatments with different SP proportions and similar crude protein (CP) content (~14%) were formulated (% of CP): 20 (S20), 30 (S30), 40 (S40) and 50 (S50). Results showed that NH3-N content increased with increasing SP levels at 4, 12 and 24 h; TVFA, acetate, propionate and valerate were higher in S30 and S40 (p < 0.05) and had quadratic effects (p < 0.05). Moreover, dry matter digestibility (DMD) and N digestibility (ND) were all decreased with S20 and S50 (p < 0.05). The S30 and S40 treatments increased the abundance of Bacteroidetes and Prevotella (Prevotella_ruminicola) but decreased the abundance of Firmicutes and Proteobacteria (p < 0.05). Bacterial pathways related to amino acid and fatty acid metabolism also were enriched with S30 and S40. The abundance of Entodinium was increased with S30 and S40 and had a positive correlation with Prevotella, and these two genera also played an important role in N metabolism and VFA synthesis of this study. In conclusion, bacterial and protozoal communities were altered by the level of SP (% of CP), with higher SP levels (~50% of CP) increasing the microbial diversity but being detrimental to rumen N metabolism.
The Role of Resveratrol in Eye Diseases—A Review of the Literature
Authors: Bryl Anna , Falkowski Mariusz , Zorena Katarzyna , Mrugacz Małgorzata
Published: July 2022

Resveratrol (3,5,4′-trans-trihydroxystilbene) is a polyphenolic phytoalexin belonging to the stilbene family. It is commonly found in grape skins and seeds, as well as other plant-based foods. Oxidative stress and inflammation play a key role in the initiation and progression of age-related eye disorders (glaucoma, cataracts, diabetic retinopathy, and macular degeneration) that lead to a progressive loss of vision and blindness. Even though the way resveratrol affects the human body and the course of many diseases is still the subject of ongoing scientific research, it has been shown that the broad spectrum of anti-inflammatory and neuroprotective properties of resveratrol has a beneficial effect on eye tissues. In our research, we decided to analyze the current scientific literature on resveratrol, its possible mechanisms of action, and its therapeutic application in order to assess its effectiveness in eye diseases.
Tight Junction Protein Expression-Inducing Probiotics Alleviate TNBS-Induced Cognitive Impairment with Colitis in Mice
Authors: Ma Xiao-Yang , Son Young-Hoo , Yoo Jong-Wook , Joo Min-Kyung , Kim Dong-Hyun
Published: July 2022

A leaky gut is closely connected with systemic inflammation and psychiatric disorder. The rectal injection of 2,4,6-trinitrobenzenesulfonic acid (TNBS) induces gut inflammation and cognitive function in mice. Therefore, we selected Bifidobacterium longum NK219, Lactococcus lactis NK209, and Lactobacillus rhamnosus NK210, which induced claudin-1 expression in TNBS- or lipopolysaccharide (LPS)-stimulated Caco-2 cells, from the fecal bacteria collection of humans and investigated their effects on cognitive function and systemic inflammatory immune response in TNBS-treated mice. The intrarectal injection of TNBS increased cognitive impairment-like behaviors in the novel object recognition and Y-maze tests, TNF-α, IL-1β, and IL-17 expression in the hippocampus and colon, and LPS level in the blood and feces, while the expression of hippocampal claudin-5 and colonic claudin-1 decreased. Oral administration of NK209, NK210, and NK219 singly or together decreased TNBS-impaired cognitive behaviors, TNF-α and IL-1β expression, NF-κB+Iba1+ cell and LPS+Iba1+ cell numbers in the hippocampus, and LPS level in the blood and feces, whereas BDNF+NeuN+ cell and claudin-5+ cell numbers and IL-10 expression increased. Furthermore, they suppressed TNBS-induced colon shortening and colonic TNF-α and IL-1β expression, while colonic IL-10 expression and mucin protein-2+ cell and claudin-1+ cell numbers expression increased. Of these, NK219 most strongly alleviated cognitive impairment and colitis. They additively alleviated cognitive impairment with colitis. Based on these findings, NK209, NK210, NK219, and their combinations may alleviate cognitive impairment with systemic inflammation by suppressing the absorption of gut bacterial products including LPS into the blood through the suppression of gut bacterial LPS production and alleviation of a leaky gut by increasing gut tight junction proteins and mucin-2 expression.
Iron Deficiency Anemia: Efficacy and Limitations of Nutritional and Comprehensive Mitigation Strategies
Authors: Kumar Shashi Bhushan , Arnipalli Shanvanth R. , Mehta Priyanka , Carrau Silvia , Ziouzenkova Ouliana
Published: July 2022

Iron deficiency anemia (IDA) has reached epidemic proportions in developing countries and has become a major global public health problem, affecting mainly 0–5-year-old children and young women of childbearing age, especially during pregnancy. Iron deficiency can lead to life-threatening loss of red blood cells, muscle function, and energy production. Therefore, the pathogenic features associated with IDA are weakness and impaired growth, motor, and cognitive performance. IDA affects the well-being of the young generation and the economic advancement of developing countries, such as India. The imbalance between iron intake/absorption/storage and iron utilization/loss culminates into IDA. However, numerous strategic programs aimed to increase iron intake have shown that improvement of iron intake alone has not been sufficient to mitigate IDA. Emerging critical risk factors for IDA include a composition of cultural diets, infections, genetics, inflammatory conditions, metabolic diseases, dysbiosis, and socioeconomic parameters. In this review, we discuss numerous IDA mitigation programs in India and their limitations. The new multifactorial mechanism of IDA pathogenesis opens perspectives for the improvement of mitigation programs and relief of IDA in India and worldwide.
Systematic Review on the Potential Effect of Berry Intake in the Cognitive Functions of Healthy People
Authors: De Amicis Ramona , Mambrini Sara Paola , Pellizzari Marta , Foppiani Andrea , Bertoli Simona , Battezzati Alberto , Leone Alessandro
Published: July 2022

The increase in life expectancy poses health challenges, such as increasing the impairment of cognitive functions. Berries show a neuroprotective effect thanks to flavonoids, able to reduce neuroinflammatory and to increase neuronal connections. The aim of this systematic review is to explore the impact of berries supplementation on cognitive function in healthy adults and the elderly. Twelve studies were included for a total of 399 participants, aged 18–81 years (mean age: 41.8 ± 4.7 years). Six studies involved young adults (23.9 ± 3.7 years), and four studies involved the elderly (60.6 ± 6.4 years). Most studies investigated effects of a single berry product, but one used a mixture of 4 berries. Non-significant differences were detected across cognition domains and methodologies, but significant and positive effects were found for all cognitive domains (attention and concentration, executive functioning, memory, motor skills and construction, and processing speed), and in most cases they were present in more than one study and detected using different methodologies. Although some limitations should be taken into account to explain these results, the positive findings across studies and methodologies elicit further studies on this topic, to endorse the consumption of berries in healthy populations to prevent cognitive decline.
Phloridzin Reveals New Treatment Strategies for Liver Fibrosis
Authors: Shi Yahong , Yan Tun , Lu Xi , Li Kai , Nie Yifeng , Jiao Chuqiao , Sun Huizhen , Li Tingting , Li Xiang , Han Dong
Published: July 2022

Liver fibrosis is an urgent public health problem which is difficult to resolve. However, various drugs for the treatment of liver fibrosis in clinical practice have their own problems during use. In this study, we used phloridzin to treat hepatic fibrosis in the CCl4-induced C57/BL6N mouse model, which was extracted from lychee core, a traditional Chinese medicine. The therapeutic effect was evaluated by biochemical index detections and ultrasound detection. Furthermore, in order to determine the mechanism of phloridzin in the treatment of liver fibrosis, we performed high-throughput sequencing of mRNA and lncRNA in different groups of liver tissues. The results showed that compared with the model group, the phloridzin-treated groups revealed a significant decrease in collagen deposition and decreased levels of serum alanine aminotransferase, aspartate aminotransferase, laminin, and hyaluronic acid. GO and KEGG pathway enrichment analysis of the differential mRNAs was performed and revealed that phloridzin mainly affects cell ferroptosis. Gene co-expression analysis showed that the target genes of lncRNA were obvious in cell components such as focal adhesions, intercellular adhesion, and cell–substrate junctions and in metabolic pathways such as carbon metabolism. These results showed that phloridizin can effectively treat liver fibrosis, and the mechanism may involve ferroptosis, carbon metabolism, and related changes in biomechanics.
The Future of Tissue-Targeted Lipid Nanoparticle-Mediated Nucleic Acid Delivery
Authors: Kularatne Ruvanthi N. , Crist Rachael M. , Stern Stephan T.
Published: July 2022

The earliest example of in vivo expression of exogenous mRNA is by direct intramuscular injection in mice without the aid of a delivery vehicle. The current state of the art for therapeutic nucleic acid delivery is lipid nanoparticles (LNP), which are composed of cholesterol, a helper lipid, a PEGylated lipid and an ionizable amine-containing lipid. The liver is the primary organ of LNP accumulation following intravenous administration and is also observed to varying degrees following intramuscular and subcutaneous routes. Delivery of nucleic acid to hepatocytes by LNP has therapeutic potential, but there are many disease indications that would benefit from non-hepatic LNP tissue and cell population targeting, such as cancer, and neurological, cardiovascular and infectious diseases. This review will concentrate on the current efforts to develop the next generation of tissue-targeted LNP constructs for therapeutic nucleic acids.
Phosphodiesterase-4 Inhibitor Roflumilast-Mediated Protective Effect in Sepsis-Induced Late-Phase Event of Acute Kidney Injury: A Narrative Review
Authors: Kazmi Imran , Al-Abbasi Fahad A. , Afzal Muhammad , Nadeem Muhammad Shahid , Altayb Hisham N. , Gupta Gaurav
Published: July 2022

Severe infections such as viral, bacterial, or fungal sepsis can cause an inflammatory response in the host, leading to organ failure and septic shock—phosphodiesterase-4 (PDE-4) inhibiting related agents from suppressing cyclic adenosine monophosphate (cAMP) degradation. Regulatory organisations have approved some substances in this category to reduce the risk of chronic obstructive pulmonary disease (COPD) exacerbations in patients with chronic bronchitis and a history of COPD exacerbations. Roflumilast has been shown to alleviate inflammatory responses, thus regulating airway inflammation. Additionally, roflumilast therapy dramatically enhanced B-cell lymphoma 2 (Bcl-2) expression, an anti-apoptotic marker lowered in septic animals. Previous research has indicated that roflumilast may help reverse sepsis-induced liver and lung harm, but whether it is also effective in reversing sepsis-induced renal impairment remains unknown. Therefore, this review determines whether roflumilast protects against renal dysfunction, inflammatory response, and apoptosis in sepsis-induced kidney damage. Additionally, we discussed the molecular mechanism through which roflumilast exerts its protective effect to uncover a possible treatment agent for sepsis-induced renal impairment.
Multiparticulate Systems of Meloxicam for Colonic Administration in Cancer or Autoimmune Diseases
Authors: Navarro-Ruíz Eva , Álvarez-Álvarez Covadonga , Peña M Ángeles , Torrado-Salmerón Carlos , Dahma Zaid , de la Torre-Iglesias Paloma Marina
Published: July 2022

The aim of this research is the development of new colonic release systems of meloxicam (MLX) a non-steroidal anti-inflammatory drug (NSAIDs) with pH and time-dependent vehicles for cancer or autoimmune diseases. The colon has a higher pH than the rest of the gastrointestinal tract (GIT) and this can be used as a modified release strategy. Eudragit® polymers are the most widely used synthetic products in the design of colonic release formulations because they might offer mucoadhesiveness and pH-dependent release. Colonic delivery systems produced with pH-dependent and permeable polymers (FS-30D) or with pH-independent and low permeability polymers (NM-30D), must dissolve at a pH range of 6.0–7.0 to delay the release of the drug and prevent degradation in the GIT, before reaching the colon. The conditions prepared to simulate a gastrointestinal transit showed the CNM multiparticulate system, composed of Eudragit® NM and cellulose, as the best release option for MLX with a more sustained release with respect to the other formulations. CNM formulation followed Higuchi and First-order release kinetics, thus MLX release was controlled by a combination of diffusion and polymers swelling/eroding processes.
Novel Cyclic Peptides for Targeting EGFR and EGRvIII Mutation for Drug Delivery
Authors: Furman Olga , Zaporozhets Alisa , Tobi Dror , Bazylevich Andrii , Firer Michael A. , Patsenker Leonid , Gellerman Gary , Lubin Bat Chen R.
Published: July 2022

The epidermal growth factor–epidermal growth factor receptor (EGF-EGFR) pathway has become the main focus of selective chemotherapeutic intervention. As a result, two classes of EGFR inhibitors have been clinically approved, namely monoclonal antibodies and small molecule kinase inhibitors. Despite an initial good response rate to these drugs, most patients develop drug resistance. Therefore, new treatment approaches are needed. In this work, we aimed to find a new EGFR-specific, short cyclic peptide, which could be used for targeted drug delivery. Phage display peptide technology and biopanning were applied to three EGFR expressing cells, including cells expressing the EGFRvIII mutation. DNA from the internalized phage was extracted and the peptide inserts were sequenced using next-generation sequencing (NGS). Eleven peptides were selected for further investigation using binding, internalization, and competition assays, and the results were confirmed by confocal microscopy and peptide docking. Among these eleven peptides, seven showed specific and selective binding and internalization into EGFR positive (EGFR+ve) cells, with two of them—P6 and P9—also demonstrating high specificity for non-small cell lung cancer (NSCLC) and glioblastoma cells, respectively. These peptides were chemically conjugated to camptothecin (CPT). The conjugates were more cytotoxic to EGFR+ve cells than free CPT. Our results describe a novel cyclic peptide, which can be used for targeted drug delivery to cells overexpressing the EGFR and EGFRvIII mutation.
Polymeric Nanosystems Applied for Metal-Based Drugs and Photosensitizers Delivery: The State of the Art and Recent Advancements
Authors: Dantas Kele Cristina Ferreira , Rosário Jânia dos Santos , Silva-Caldeira Priscila Pereira
Published: July 2022

Nanotechnology-based approaches for targeting the delivery and controlled release of metal-based therapeutic agents have revealed significant potential as tools for enhancing the therapeutic effect of metal-based agents and minimizing their systemic toxicities. In this context, a series of polymer-based nanosized systems designed to physically load or covalently conjugate metal-based therapeutic agents have been remarkably improving their bioavailability and anticancer efficacy. Initially, the polymeric nanocarriers were applied for platinum-based chemotherapeutic agents resulting in some nanoformulations currently in clinical tests and even in medical applications. At present, these nanoassemblies have been slowly expanding for nonplatinum-containing metal-based chemotherapeutic agents. Interestingly, for metal-based photosensitizers (PS) applied in photodynamic therapy (PDT), especially for cancer treatment, strategies employing polymeric nanocarriers have been investigated for almost 30 years. In this review, we address the polymeric nanocarrier-assisted metal-based therapeutics agent delivery systems with a specific focus on non-platinum systems; we explore some biological and physicochemical aspects of the polymer–metallodrug assembly. Finally, we summarize some recent advances in polymeric nanosystems coupled with metal-based compounds that present potential for successful clinical applications as chemotherapeutic or photosensitizing agents. We hope this review can provide a fertile ground for the innovative design of polymeric nanosystems for targeting the delivery and controlled release of metal-containing therapeutic agents.
Molecular and Nano-Structural Optimization of Nanoparticulate Mn2+-Hexarhenium Cluster Complexes for Optimal Balance of High T1- and T2-Weighted Contrast Ability with Low Hemoagglutination and Cytotoxicity
Authors: Akhmadeev Bulat Salavatovich , Nizameev Irek R. , Kholin Kirill V. , Voloshina Alexandra D. , Gerasimova Tatyana P. , Gubaidullin Aidar T. , Kadirov Marsil K. , Ismaev Ildus E. , Brylev Konstantin A. , Zairov Rustem R. , Mustafina Asiya R.
Published: July 2022

The present work introduces rational design of nanoparticulate Mn(II)-based contrast agents through both variation of the μ3 (inner) ligands within a series of hexarhenium cluster complexes [{Re6(μ3-Q)8}(CN)6]4− (Re6Q8, Q = S2−, Se2− or Te2−) and interfacial decoration of the nanoparticles (NPs) K4−2xMnxRe6Q8 (x = 1.3 − 1.8) by a series of pluronics (F-68, P-123, F-127). The results highlight an impact of the ligand and pluronic for the optimal colloid behavior of the NPs allowing high colloid stability in ambient conditions and efficient phase separation under the centrifugation. It has been revealed that the K4−2xMnxRe6Se8 NPs and those decorated by F-127 are optimal from the viewpoint of magnetic relaxivities r1 and r2 (8.9 and 10.9 mM−1s−1, respectively, at 0.47 T) and low hemoagglutination activity. The insignificant leaching of Mn2+ ions from the NPs correlates with their insignificant effect on the cell viability of both M-HeLa and Chang Liver cell lines. The T1- and T2-weighted contrast ability of F-127–K4−2xMnxRe6Q8 NPs was demonstrated through the measurements of phantoms at whole body 1.5 T scanner.
Adherence to and Persistence with Adalimumab Therapy among Swedish Patients with Crohn’s Disease
Authors: Liu Yifei , Söderberg Joakim , Chao Jingdong
Published: July 2022

Objectives: (1) to determine the adherence and persistence rates of adalimumab therapy among Swedish patients with Crohn’s disease (CD), and (2) to compare self-administration devices to predict the medication adherence and persistence. Methods: We conducted a retrospective analysis of the Swedish National Board of Health and Welfare database during a unique time period, when both the pen and the syringe were available. The pen was proposed to indicate a larger extent of internal control, according to health locus of control. Medication adherence was defined as a medication possession ratio (MPR) ≥ 0.8. A patient was considered nonpersistent if the time between any two dispensing records, minus the days of supply dispensed exceeded 180 days. The predictors of adherence were evaluated using a logistic regression, and the predictors of persistence were evaluated using a Cox proportional hazards model. Results: Among the 1083 patients studied, 89% were adherent and 77% were persistent. The patients using the pen and the patients treated in gastroenterology centers were more likely to be adherent and less likely to be nonpersistent. Conclusions: The adherence rate to adalimumab therapy was 89% and the one-year persistence rate was 70%. The pen and treatment in a gastroenterology center had a positive impact on the adherence and persistence among Swedish patients with CD.
Impacts of Intercropped Maize Ecological Shading on Tea Foliar and Functional Components, Insect Pest Diversity and Soil Microbes
Authors: Zou Yan , Shen Fangyuan , Zhong Yanni , Lv Changning , Pokharel Sabin Saurav , Fang Wanping , Chen Fajun
Published: July 2022

Ecological shading fueled by maize intercropping in tea plantations can improve tea quality and flavor, and efficiently control the population occurrence of main insect pests. In this study, tea plants were intercropped with maize in two planting directions from east to west (i.e., south shading (SS)) and from north to south (i.e., east shading (ES) and west shading (WS)) to form ecological shading, and the effects on tea quality, and the population occurrence and community diversity of insect pests and soil microbes were studied. When compared with the non-shading control, the tea foliar nutrition contents of free fatty acids have been significantly affected by the ecological shading. SS, ES, and WS all significantly increased the foliar content of theanine and caffeine and the catechin quality index in the leaves of tea plants, simultaneously significantly reducing the foliar content of total polyphenols and the phenol/ammonia ratio. Moreover, ES and WS both significantly reduced the population occurrences of Empoasca onukii and Trialeurodes vaporariorum. Ecological shading significantly affected the composition of soil microbial communities in tea plantations, in which WS significantly reduced the diversity of soil microorganisms.
The Computational Preventive Potential of the Rare Flavonoid, Patuletin, Isolated from Tagetes patula, against SARS-CoV-2
Authors: Metwaly Ahmed M. , Elkaeed Eslam B. , Alsfouk Bshra A. , Saleh Abdulrahman M. , Mostafa Ahmad E. , Eissa Ibrahim H.
Published: July 2022

The rare flavonoid, patuletin, was isolated from the flowers of Tagetes patula growing in Egypt. The rarity of the isolated compound inspired us to scrutinize its preventive effect against COVID-19 utilizing a multi-step computational approach. Firstly, a structural similarity study was carried out against nine ligands of nine SARS-CoV-2 proteins. The results showed a large structural similarity between patuletin and F86, the ligand of SARS-CoV-2 RNA-dependent RNA polymerase (RdRp). Then, a 3D-Flexible alignment study of patuletin and F86 verified the proposed similarity. To determine the binding opportunity, patuletin was docked against the RdRp showing a correct binding inside its active pocket with an energy of −20 kcal/mol that was comparable to that of F86 (−23 kcal/mol). Following, several MD simulations as well as MM-PBSA studies authenticated the accurate binding of patuletin in the RdRp via the correct dynamic and energetic behaviors over 100 ns. Additionally, in silico ADMET studies showed the general safety and drug-likeness of patuletin.
Effects of Mechanical Stress on Insulation Structure and Performance of HV Cable
Authors: Su Jingang , Wei Liqiang , Zheng Jingquan , Liu Jiahao , Zhang Peng , Pang Xianhai , Xing Yunqi
Published: July 2022

Mechanical stresses generated during manufacturing and laying process of high voltage cables can result in degradation of insulation properties, affecting the stable operation of the transmission system. Traditional test methods for testing the effect of mechanical stress on the insulation properties of polyethylene still have some shortcomings to be explored and it is able to explain the changes of the insulation properties of polyethylene under mechanical stress from a microscopic perspective. In order to further study the effect of stress on the insulation properties of polyethylene, microstructural changes, the breakdown field strength, conductivity and charge distribution of polyethylene at different elongation rates are investigated by a combination of experimental and molecular dynamics simulations. The results show that the increase in stress leads to a decrease in crystallinity and microcrystalline size of the material decrease. The untwisting and orientation of the polyethylene molecular chains during the stretching process can create cavities, resulting in an uneven sample distribution and thickness reduction, leading to a reduction in the breakdown field strength. Meanwhile, some crystal regions are transformed into amorphous regions. The loose amorphous regions facilitate the directional migration of carriers, resulting in the increase of conductivity. When the elongation ratio is smaller, the distance between the molecular chains increases and the trap depth of the specimen becomes shallower. This facilitates the migration of ions and electrons and increases the rate of decay of the surface potential. When the stretch is further increased, new traps are created by broken molecular chains to limit the movement of charges, decreasing the decay rate of the surface potential and reducing the insulation properties of the polyethylene. Meanwhile, the molecular dynamics model of semi-crystalline polyethylene was developed to observe the microstructure and energy changes during the stretching process. The conclusions in terms of tensile tests were verified from a microscopic perspective.
Applications of Electrospun Drug-Eluting Nanofibers in Wound Healing: Current and Future Perspectives
Authors: Akombaetwa Nakamwi , Bwanga Alick , Makoni Pedzisai Anotida , Witika Bwalya A.
Published: July 2022

Wounds are a consequence of disruption in the structure, integrity, or function of the skin or tissue. Once a wound is formed following mechanical or chemical damage, the process of wound healing is initiated, which involves a series of chemical signaling and cellular mechanisms that lead to regeneration and/or repair. Disruption in the healing process may result in complications; therefore, interventions to accelerate wound healing are essential. In addition to mechanical support provided by sutures and traditional wound dressings, therapeutic agents play a major role in accelerating wound healing. The medicines known to improve the rate and extent of wound healing include antibacterial, anti-inflammatory, and proliferation enhancing agents. Nonetheless, the development of these agents into eluting nanofibers presents the possibility of fabricating wound dressings and sutures that provide mechanical support with the added advantage of local delivery of therapeutic agents to the site of injury. Herein, the process of wound healing, complications of wound healing, and current practices in wound healing acceleration are highlighted. Furthermore, the potential role of drug-eluting nanofibers in wound management is discussed, and lastly, the economic implications of wounds as well as future perspectives in applying fiber electrospinning in the design of wound dressings and sutures are considered and reported.
Synthesis and Characterization of New Polycarbonate-Based Poly(thiourethane-urethane)s
Authors: Puszka Andrzej , Sikora Janusz W.
Published: July 2022

The new segmented poly(thiourethane-urethane)s (PTURs) based on 1,1′-methanediylbis(4-isocyanatocyclohexane) (HMDI, Desmodur W®), polycarbonate diol (PCD, Desmophen C2200) and (methanediyldibenzene-4,1-diyl)dimethanethiol were synthesized by one-step melt polyaddition method. The obtained PTURs, with a content of 30–60 wt% of the hard segments (HS), were tested in which the influence of changes in the HS content on their properties was determined. The polymers were characterized by Fourier transform infrared spectroscopy (FTIR), gel permeation chromatography (GPC), thermal analysis (DSC, TGA) and thermomechanical analysis (DMTA). Additionally, tensile strength, optical (refractive index, UV-VIS and color) and surface properties of the obtained polymers (contact angle and surface free energy) and adhesion to copper were examined. FTIR analysis verified the supposed structure of the polymers obtained and showed a complete conversion of the isocyanate groups. TGA analysis confirmed the relatively good thermal stability of the polymers. On the other hand, after performing the DSC analysis, it was possible to state that the obtained materials were partially or completely amorphous, and the microphase separation decreased with increasing HS content in the polymer. Similar observations were made from the DMTA data. In addition, the hardness, tensile strength, modulus of elasticity, storage modulus, adhesion to copper, refractive index and total free surface energy increased with increasing HS content in the polymer.
Composite Coatings for Osteoblast Growth Attachment Fabricated by Matrix-Assisted Pulsed Laser Evaporation
Authors: Grumezescu Valentina , Grumezescu Alexandru , Ficai Anton , Negut Irina , Vasile Bogdan , Gălățeanu Bianca , Hudiță Ariana
Published: July 2022

The bioactive and biocompatible properties of hydroxyapatite (HAp) promote the osseointegration process. HAp is widely used in biomedical applications, especially in orthopedics, as well as a coating material for metallic implants. We obtained composite coatings based on HAp, chitosan (CS), and FGF2 by a matrix-assisted pulsed laser evaporation (MAPLE) technique. The coatings were physico-chemically investigated by means of X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), Infrared Microscopy (IRM), and Scanning Electron Microscopy (SEM). Further, biological investigations were performed. The MAPLE-composite coatings were tested in vitro on the MC3T3-E1 cell line in order to endorse cell attachment and growth without toxic effects and to promote pre-osteoblast differentiation towards the osteogenic lineage. These coatings can be considered suitable for bone tissue engineering applications that lack toxicity and promotes cell adhesion and proliferation while also sustaining the differentiation of pre-osteoblasts towards mature bone cells.
Low-Velocity Impact Behavior of Sandwich Plates with FG-CNTRC Face Sheets and Negative Poisson’s Ratio Auxetic Honeycombs Core
Authors: Yang Chunhao , Ma Wuning , Zhang Zhendong , Zhong Jianlin
Published: July 2022

The combination of auxetic honeycomb and CNT reinforcement composite is expected to further improve the impact protection performance of sandwich structures. This paper studies the low-velocity impact response of sandwich plates with functionally graded carbon nanotubes reinforced composite (FG-CNTRC) face sheets and negative Poisson’s ratio (NPR) auxetic honeycomb core. The material properties of FG-CNTRC were obtained by the rule of mixture theory. The auxetic honeycomb core is made of Ti-6Al-4V. The governing equations are derived based on the first-order shear deformation theory and Hamilton’s principle. The nonlinear Hertz contact law is used to calculate the impact parameters. The Ritz method with Newmark’s time integration schemes is used to solve the response of the sandwich plates. The (20/−20/20)s, (45/−45/45)s and (70/−70/70)s stacking sequences of FG-CNTRC are considered. The effects of the gradient forms of FG-CNTRC surfaces, volume fractions of CNTs, impact velocities, temperatures, ratio of plate length, width and thickness of surface layers on the value of the plate center displacement, the recovery time of deformation, contact force and contact time of low-velocity impact were analyzed in detail.
Flexural Behavior of Full-Scale Damaged Hollow RC Beams Strengthened with Prestressed SCFRP Plate under Four-Point Bending
Authors: Li Baojun , Zeng Lingkai , Guo Xinyan , Wang Yilin , Deng Zhiheng
Published: July 2022

The advantages of using prestressed carbon fiber reinforced polymer (CFRP) for strengthening and retrofitting structures have been reported in recent years. In this regard, most of the studies on prestressed CFRP technique have been carried out in the laboratory test with small-scale and no damage (reinforced concrete) RC beam. However, the real structures that need to be retrofitted in service are often degraded or damaged due to early cracking. This paper aims at studying the effect of prestressed CFRP method on full-scale and damaged RC beams. The damaged levels of four full-scale damaged hollow RC beams taken from an old bridge were evaluated. One damaged beam was tested to check the residual capacity, and the other three were strengthened with prestressed composite strengthened CFRP and steel-carbon fiber reinforced polymer (SCFRP). The flexural behavior of non-strengthened and prestressed strengthened beams was investigated. During the experiments, the failure modes, deflection, yield and ultimate load, strains of concrete, steel reinforcements, and SCFRP were measured and analyzed. The results showed that the stiffness at the elastic stage was increased by 64.9%, 66.9%, and 67.1% after strengthened by SCFRP with 30%, 40%, and 60% prestressing level. Moreover, the ultimate load of damaged hollow RC beams were improved by 19.53%, 21.82%, and 31.9%, respectively. The flexural behavior of the severely damaged RC beam with strength reduction coefficient of 0.65 can be recovered after being strengthened by SCFRP with 40% prestressing levels. Meanwhile, SCFRP-concrete interface debonding failure occurred when the prestressing level exceed 60%, and the characteristics of brittle failure became more evident with increased prestressing level of the SCFRP.
Functionalization of Electrospun Nanofiber for Bone Tissue Engineering
Authors: Yan Xuan , Yao Haiyan , Luo Jun , Li Zhihua , Wei Junchao
Published: July 2022

Bone-tissue engineering is an alternative treatment for bone defects with great potential in which scaffold is a critical factor to determine the effect of bone regeneration. Electrospun nanofibers are widely used as scaffolds in the biomedical field for their similarity with the structure of the extracellular matrix (ECM). Their unique characteristics are: larger surface areas, porosity and processability; these make them ideal candidates for bone-tissue engineering. This review briefly introduces bone-tissue engineering and summarizes the materials and methods for electrospining. More importantly, how to functionalize electrospun nanofibers to make them more conducive for bone regeneration is highlighted. Finally, the existing deficiencies of functionalized electrospun nanofibers for promoting osteogenesis are proposed. Such a summary can lay the foundation for the clinical practice of functionalized electrospun nanofibers.
Nanoindentation of Multifunctional Smart Composites
Authors: Zhang Zhenxue , Bellisario Denise , Quadrini Fabrizio , Jestin Simon , Ravanelli Francesca , Castello Mauro , Li Xiaoying , Dong Hanshan
Published: July 2022

Three multifunctional smart composites for next-generation applications have been studied differently through versatile nanoindentation investigation techniques. They are used in order to determine peculiarities and specific properties for the different composites and to study the charge/matrix, charge/surface, or smart functions interactions. At first, a mapping indentation test was used to check the distribution of hardness and modulus across a large region to examine any non-uniformity due to structural anomalies or changes in properties for a carbon nanotubes (CNTs)-reinforced polypropylene (PP V-2) nanocomposite. This smart composite is suitable to be used in axial impeller fans and the results can be used to improve the process of the composite produced by injection moulding. Secondly, the interfacial properties of the carbon fibre (CF) and the resin were evaluated by a push-out method utilizing the smaller indentation tip to target the individual CF and apply load to measure its displacement under loads. This is useful to evaluate the effectiveness of the surface modification on the CFs, such as sizing. Finally, nanoindentation at different temperatures was used for the probing of the in situ response of smart shape memory polymer composite (SMPC) usable in grabbing devices for aerospace applications. Furthermore, the triggering temperature of the shape memory polymer response can be determined by observing the change of indentations after the heating and cooling cycles.
Progressive Damage Behaviour Analysis and Comparison with 2D/3D Hashin Failure Models on Carbon Fibre–Reinforced Aluminium Laminates
Authors: Hu Haichao , Wei Qiang , Liu Boya , Liu Yun , Hu Ning , Ma Quanjin , Wang Chuancai
Published: July 2022

It is known that carbon fibre–reinforced aluminium laminate is the third generation of fibre metal materials. This study investigates the response of carbon fibre–reinforced aluminium laminates (CARALL) under tensile loading and three-point bending tests, which evaluate the damage initiation and propagation mechanism. The 2D Hashin and 3D Hashin VUMAT models are used to analyse and compare each composite layer for finite element modelling. A bilinear cohesive contact model is modelled for the interface failure, and the Johnson cook model describes the aluminium layer. The mechanical response and failure analysis of CARALL were evaluated using load versus deflection curves, and the scanning electron microscope was adopted. The results revealed that the failure modes of CARALL were mainly observed in the aluminium layer fracture, fibre pull-out, fracture, and matrix tensile fracture under tensile and flexural loading conditions. The 2D Hashin and 3D Hashin models were similar in predicting tensile properties, flexural properties, mechanical response before peak load points, and final failure modes. It is highlighted that the 3D Hashin model can accurately reveal the failure mechanism and failure propagation mechanism of CARALL.
Special Issue: “Emerging Technologies in Heating, Ventilation, Air Conditioning and Refrigeration (HVAC&R) Systems”
Authors: Yang Kai-Shing , Shyu Jin-Cherng , Lee Ming-Tsang
Published: July 2022

Heating, ventilation, air conditioning and refrigeration (HVAC&R) systems provide physical environment comfort for the occupants at a possible expense of large amount of energy consumption [...]
Performance Comparison and Optimization of 16V265H Diesel Engine Fueled with Biodiesel Based on Miller Cycle
Authors: Jiang Feng , Zhou Junming , Hu Jie , Tan Xueyou , Mo Qinglie , Cao Wentong
Published: July 2022

This paper introduces the theoretical basis and optimization method of diesel engine working process theory. By comparing two Miller cycle schemes of B20 biodiesel under different load conditions of 1000 rpm (100%, 75%, and 50%), the best Miller cycle scheme and the best Miller degree were found. Then, based on the Miller cycle scheme, its performance was optimized and analyzed, and the best intake timing scheme of the B20 biodiesel engine under different working conditions was obtained. The results show that the performance of B20 biodiesel in variable valve overlap angle Miller cycle is better than that in variable cam profile Miller cycle, and the effect is the best when the Miller degree is 30 °CA. When B20 biodiesel is used under 100% and 50% load conditions, the maximum power under the two loads is in the area near intake valve timing 179 °CAA and exhaust valve timing 174 °CAA, and intake valve timing 224.5 °CAA and exhaust valve timing 119 °CAA, respectively. Fuel consumption, soot emissions, and NOx emissions also have the corresponding best performance intake valve and exhaust valve positions.
Micro Pilgrimages: A New Post-Secular Trend?
Authors: Bailey Anne E.
Published: July 2022

The word “micro” or “mini” is increasingly appearing in relation to pilgrimage. A Guardian article in December 2021 described a guided pilgrimage walk in Sussex as a “micro pilgrimage”; in the spring of 2022, six “micro pilgrimages” took place in southern England. However, what is a micro pilgrimage, and what has prompted its sudden surge in popularity? This article explores this seemingly innovative practice, focusing on Britain, where it is particularly prevalent. It shows how and why micro pilgrimages became particularly prominent during the COVID-19 pandemic, and discusses their continuing popularity today. A micro pilgrimage is usually understood as a pilgrimage which is completed in one day or less, an idea—the article argues—which has arisen from assumptions that short pilgrimages are a departure from the norm. The article challenges this notion by showing that, throughout Western history, long journeys have been in the minority. However, while arguing that micro pilgrimages have been with us since the Middle Ages, the article also recognizes that there is much which is indeed new about modern forms of the practice.
Implementation of Circular Business Models for Olive Oil Waste and By-Product Valorization
Authors: Donner Mechthild , Radić Ivana , Erraach Yamna , El Hadad-Gauthier Fatima
Published: July 2022

Mediterranean olive oil producers have multiple incentives for adopting circular business models and better resource management, facing water scarcity and huge amounts of waste, but also seeing new opportunities for value creation. This article aimed to understand how circular business models valorizing olive oil waste and by-products are implemented. Ten business cases from six Mediterranean countries were studied, mainly based on semi-structured interviews with enterprise managers. Data were analyzed according to the business model canvas elements, success factors, and barriers while considering the institutional context. The results highlight the diversity of activities, types of resources used, and partnerships and products offered in different market segments. The principles of biowaste conversion and circularity, such as cascading, upcycling, recycling, and recovering, are all applied. The key success factors comprise an environmental concern, knowledge about biotechnologies, markets and logistics, a long-term commitment to the sector, local availability of resources, legislation, subsidies, and product acceptance by consumers. The main barriers include a lack of specific public financial support, an insufficient knowledge transfer from research to olive oil producers, and a lack of articulation of needs for research by the enterprises. More public-private collaborations and multi-stakeholder projects are needed for further shifting to a circular economy in the olive oil sector.
Remote Sensing Application for Landslide Detection, Monitoring along Eastern Lake Michigan (Miami Park, MI)
Authors: Sataer Guzalay , Sultan Mohamed , Emil Mustafa Kemal , Yellich John A. , Palaseanu-Lovejoy Monica , Becker Richard , Gebremichael Esayas , Abdelmohsen Karem
Published: July 2022

We assessed the nature and spatial and temporal patterns of deformation over the Miami Park bluffs on the eastern margin of Lake Michigan and investigated the factors controlling its observed deformation. Our approach involved the following steps: (1) extracting bluff deformation rates (velocities along the line of sight of the satellite) using a stack of Sentinel-1A radar imagery in ascending acquisition geometry acquired between 2017 and 2021 and applying the Intermittent Small Baseline Subset (ISBAS) InSAR time series analysis method; (2) generating high-resolution (5 cm) elevation models and orthophotos from temporal unmanned aerial vehicle (UAV) surveys acquired in 2017, 2019, and 2021; and (3) comparing the temporal variations in mass wasting events to other relevant datasets including the ISBAS-based bluff deformation time series, lake level (LL) variations, and local glacial stratigraphy. We identified areas witnessing high line-of-sight (LOS) deformation rates (up to −21 mm/year) along the bluff from the ISBAS analysis and seasonal deformation patterns associated with freeze-thaw cycles, suggesting a causal effect. The acceleration of slope failures detected from field and UAV acquisitions correlated with high LLs and intensified onshore wave energy in 2020. The adopted methodology successfully predicts landslides caused by freezes and thaws of the slope face by identifying prolonged slow deformation preceding slope failures, but it does not predict the catastrophic landslides preceded by short-lived LOS deformation related to LL rise.