bluuebunny/crossref_metadata_2025 huggingface dataset:
Gambaran Sanitasi Tempat Pengelolaan Pangan di Wilayah Perimeter Pelabuhan Tanjungwangi Banyuwangi 2022
Authors: Aisyaroh Septia Hilda , Yudhastuti Ririh
Published: December 2023

Latar Belakang: Tempat Pengelolaan Pangan (TPP) merupakan tempat yang digunakan sebagai produksi untuk pangan olahan siap saji mulai dari persiapan, pengolahan, pengemasan, penyimpanan, penyajian hingga pengangkutan yang dilakukan pengawasan termasuk pada area pelabuhan. Pelabuhan merupakan tempat bertemunya orang banyak dari berbagai daerah karena pelabuhan merupakan pintu masuk kedalam suatu wilayah, sehingga dapat berpotensi menyebarkan penyakit yang dapat mengakibatkan kedaruratan kesehatan.
Tujuan: Tujuan dari penelitian ini yaitu untuk mengetahui gambaran sanitasi TPP di wilayah perimeter Pelabuhan Tanjungwangi Banyuwangi.
Metode: Rancangan penelitian ini menggunakan jenis rancangan Cross-Sectional Study. Teknik pengambilan data dilakukan dengan cara observasi dan wawancara. Pengambilan sampel menggunakan total populasi di wilayah perimeter Pelabuhan Tanjungwangi yaitu sebanyak 6 TPP yang diobservasi. Penilaian variabel menggunakan perhitungan skor dari semua variabel dengan nilai minimum 80 sesuai dengan dengan Peraturan Menteri Kesehatan Republik Indonesia Nomor 14 Tahun 2021 tentang Standar Kegiatan Usaha dan Produk pada Penyelenggaraan Perizinan Berusaha Berbasis Risiko Sektor Kesehatan.
Hasil: Hasil dari penelitian ini yaitu 6 warung makan yang berada di wilayah pelabuhan Tanjungwangi telah memenuhi persyaratan batas minimal Inspeksi Kesehatan Lingkungan, yaitu diatas nilai 80.
Kesimpulan: Kesimpulan dari penelitian ini yaitu warung makan yang ada di pelabuhan Tanjungwangi memiliki risiko rendah terjadinya penularan penyakit akibat sanitasi makanan yang mengakibatkan kedaruratan kesehatan.
(Des)conformidades entre o trombone tenor e o trombone baixo: aspectos históricos, estruturais e atribuições técnicas/performáticas musicais em obras sinfônicas
Authors: Decarli Fransoel , Ronqui Paulo
Published: December 2023

O presente artigo apresenta o relato de uma investigação acerca da história, da estrutura, das atribuições musicais e dos aspectos técnicos do trombone tenor e do trombone baixo. Com o intuito de preencher parte da lacuna existente na pesquisa acadêmica nacional, o objetivo principal deste artigo é ofertar dados que proporcionem uma melhor compreensão a respeito de conceitos formulados para os trombones tenor e baixo. Para esse fim, fundamentou-se na revisão da literatura nacional e estrangeira que apresenta conteúdos relacionados aos trombones, em tratados de orquestração e instrumentação, em entrevistas com professores de trombones de instituições de ensino superior nacionais e um estudo analítico de selecionadas composições. Como resultado, oferta-se dados históricos e estruturais sobre ambos os trombones, detalhes das funções exercidas na música de concerto e destacados aspectos técnicos relacionados às válvulas rotativas, à tessitura e à sonoridade. Mesmo que este estudo corrobore para a inferência de que se tratam de instrumentos similares, nota-se que são diferentes, uma vez que apresentam quesitos específicos de execução.
Pengaruh Aktivitas Penggunaan Energi terhadap Emisi CO2 (Studi Kasus: Sembilan Negara dengan Kumulatif Emisi Terbesar)
Authors: Shereen Shereen , Laulita Nasar Buntu
Published: December 2023

The decline of the living environment is caused by the increasing pollution and damage to the environment, especially CO2 emissions. Given this situation, developed countries in the world are worried about excessive carbon dioxide emissions, because CO2 emissions are directly related to economic growth and energy consumption. Three sectors that have the highest emissions are the electricity sector at 42%, the transportation sector at 23%, and the housing sector at 6%. The purpose of this study was to examine the effect of energy consumption, gross domestic product, international trade, foreign direct investment, and urbanization on CO2 emissions from 1990-2014. The examination focused on countries, like the United States, China, Russia, Brazil, Indonesia, Germany, India, England. and Japan. To achieve this aim, this research used a quantitative approach. The data were gathered from secondary data obtained through a literature study on World Bank data. It involved panel data totaling 225 observations. The data were analyzed using panel linear regression analysis with the Fixed Effect Model (FEM). The results showed that the variables, such as energy and urbanization, had a positive and significant effect on CO2 emissions. Meanwhile, gross domestic product, international trade, and foreign direct investment had a negative and significant effect on CO2 emissions. Simultaneously, the independent variables affected the dependent variable.
Cultural and Physiological Characteristics of Alternaria alternata
Authors: Shohrat Naila , Karegowda C. , Narayanswamy H.
Published: December 2023

Tobacco is one of the major important commercial crops grown throughout the country. Brown spot caused by Alternaria alternata is one of the major destructive disease causes heavy losses under field condition. Studies on cultural and physiological features of the pathogen are of immense use in understanding the nature of the pathogen. The radial growth of A. alternata was maximum on potato dextrose agar with colony diameter of 89.00 mm followed by Czapek’s dox agar (87.00 mm) colour of the mycelium varied from whitish to dark grey. Physiological studies revealed that growth of the pathogen was maximum at temperature of 25°C (329.13 mg) and lowest at 5°C (67.16 mg), while the maximum dry weight was observed at pH 6.5 (586.53 mg).
Effect of Micronutrients on Growth, Tuber Yield and Quality of Cassava (Manihot esculenta Crantz)
Authors: Maradana Janaki , Ashok P. , Priyanka A. Pavani
Published: December 2023

Cassava is one of the important tuber crops grown in marginal lands for its edible tuberous roots. Besides macro nutrient deficiencies, some micronutrient deficiencies are observed in cassava. To overcome those micronutrient deficiencies as well as to increase the cassava tuber yield and tuber quality, a field experiment was laid out during 2014-15, 2015-16 and 2016-17 at Dr. YSRHU - Horticultural Research Station, Peddapuram with eight treatments in randomized block design with three replications. The results revealed that the significant differences were observed between the treatments for all studied characters. The maximum plant height and stem girth were observed in T7 (POP + MgSO4 @ 20 kg ha-1 + ZnSO4 @12.5 kg ha-1 + Borax @10 kg ha-1 @ 20 kg ha-1 + FeSO4 (0.5 % stake dipping)). The maximum tuber yield per hectare and starch content were also recorded in T7 with 42.59 t/ha and 27.63 per cent followed by T6 (POP + MgSO4 + ZnSO4 + Borax) with 38.29 t/ha and T5 (POP + FeSO4 (0.5 % stake dipping) with 27.26 per cent respectively. The highest B:C ratio was recorded for T7 whereas it was lowest for T8 (Absolute control).
Partial Purification and Characterization of Extracellular Lipoprotein Lipase (ELPL) Obtained from Lysinibacillus fusiformis L-52 Bacteria Isolated from Dairy Industry Wastes
Authors: Jaganmai G. , Jinka Rajeswari , Vadde Ramakrishna
Published: December 2023

Among various industrial enzymes, lipases have an important role in a wide variety of industrial applications. There are very few reports, that lipase was obtained from Lysinibacillus fusiformis L-52 bacteria isolated from dairy industry effluents. This Extracellular Lipoprotein Lipase (ELPL) released from the isolated bacteria was purified partially by using ammonium sulfate precipitation and Sephadex G-150 column chromatography. The purified enzyme was found to be a lipid-containing protein with a molecular weight of 69.89kDa approximately. It has shown the lipolytic activity on various substrates, specifically on natural substrates like olive oil and sunflower oil, and on synthetic substrate 4-para Nitro Phenyl Palmitate (pNPP). ELPL showed a Km value of 0.045mM with pNPP as substrate and has an optimum temperature at 370C, and optimum pH of 7.0. The enzyme was found to be active in hydrolyzing dairy effluent and also serum lipids. ELPL also showed a significant effect in hydrolyzing plant-based lipids containing monounsaturated fatty acids like oleic acid and linoleic acid but not on animal fats like butter.
Physical, Functional and Nutraceutical Properties of Composite Flour Cookies
Authors: Wadakappanavar S. Akkavva , Xiang Jinie , Beta Trust , Koksel Filiz , B. Yenagi Nirmala
Published: December 2023

Composite flours obtaining by blending cereal or legume flours and other functional ingredients can be used in the preparation of baked foods with superior quality. In this study cookies made from finger millet, little millet, horse gram and refined wheat flour were characterised for their physical, functional and chemical properties. Color values of composite flour, finger millet and horse gram flour were darker and yellower compared to little millet flour and refined wheat flour. Sprouting increased water solubility and water binding capacity of flours. Oil absorption capacity was higher in composite flour compared to individual flours. As the proportion of composite flour increased, there was a gradual decrease in the diameter and increase in the thickness, hardness, darkness, total phenolic content and antioxidant activity of the cookies. Cookies were acceptable up to 50% level of incorporation of composite flour in cookies. Polyphenol and antioxidant activity were positively and significantly correlated. Further research is needed to better understand the impact of intervention on improvement in the nutritional profile of school children. Cookies made from germinated composite flours can be an alternative to wheat flour cookies. Incorporation of millet based composite flour in cookies improves the nutritional quality. Significance and novelty: Consumption of millets could overcome the problem of poor nutrition among school children and helps to combat micronutrient deficiencies.
Bile Salt Administration Ameliorated Polymicrobial Sepsis in Mice by Countering Oxidative Damage, Inflammation and Bacterial Load
Authors: Tyagi Anuradha , Gupta Vanita
Published: December 2023

Sepsis is a life threatening systemic inflammatory response to an infection or tissue damage. Bile salts are known to maintain intestinal barrier function. The current study investigated the protective role of bile salt against sepsis-induced lethality. Cecal slurry prepared from donor mice was administered intra-peritoneally to C57BL/6 male mice to induce sepsis. Animals subjected to cecal slurry were given either saline or bile salt (8 and 16 mg/ kg b.wt) intravenously 2 h before cecal slurry administration and observed for 10 days for survival, bacterial load, and hematological changes. Changes in oxidant-antioxidant level, inflammation, and histology were observed in lung, liver, and kidney. Acute toxicity of bile salt was conducted in accordance to OECD guidelines. Results showed that cecal slurry administration induced sepsis in mice as seen by increased bacterial load in peritoneal fluid and blood. 16 mg/kg b.wt of bile salt increased the survival rate against sepsis from 10% to 50% and diminished the bacterial load in peritoneal fluid and blood. Bile salt administration significantly (P < 0.05) ameliorated the cecal slurry induced increase in lipid peroxidation and myeloperoxidase activity; decrease in total thiol, catalase, and superoxide dismutase levels, spike in WBC count, and altered histological changes such as leukocyte infiltration and hemorrhage. Acute toxicity study of bile extract revealed no lethality along with any significant change in body weight. The findings of the current study indicate that bile salt administration increased survival rate possibly by effective bacterial clearance and protecting tissues from oxidative damage and inflammation.
Studies on Use of Medicinal Plant Extracts as Priming Treatments for Healthy Germination and Seedling Growth of Bhima Red and Bhima Super Varieties in Onion
Authors: Ryavalad Shivayogi , Naveenkumar B. S. , Golagi S. G.
Published: December 2023

The present investigation of studies on the use of medicinal plant extracts as priming treatments for healthy germination and seedling growth in onion was carried out at the seed testing Laboratory, University of Horticultural Sciences Bagalkot. The varieties used in this study were Bhima red and Bhima super. The priming treatment of seeds has been carried on the varieties Bhima red and Bhima super with 4 treatments and 4 replications and treating the seeds with the extracts of Aloe vera (Aloe barbadensis), Calotropis (Calotropis gigantea), Neem (Azadiracta indica), Tulsi (Ocimum sanctum) with two different concentration of 0.25 and 0.50 % and primed seeds were kept in the incubator for 15 days and the seed germination, growth of shoot and root were measured. Variation in the growth has been significantly compared. The highest germination was recorded from Tulsi plant extract followed by the Aloe vera, Neem and Calotropis. The length of the root and shoot was recorded highest in the Tulsi followed by Aloe vera, Neem and calotropis.
Impact of Certain Chemical Insecticides Along with Biopesticide on Helicoverpa armigera (Hubner) in Chickpea
Authors: P. Basugade Rushikesh , M. Bondre Chetan , A. Gawali Kevin , S. Jeur Viresh
Published: December 2023

To investigate the impact of chemical insecticide along with biopesticides on gram pod borer Helicoverpa armigera in chickpea conducted infield condition of G.H.R.U.School of Agriculture Sciences, Saikheda (M.P.) with various treatments. The trials laid out as per experimental design RBD with seven treatment modules including control i.e. Flubendiamide 480SC, Indoxacarb 14.5SC, Spinosad 45SC, HaNPV 250LE + Btk 8L, Novaluron 10EC, HaNPV 250LE. Respectively three spray schedules taken on gram pod borer and after application of all sprays Flubendiamide 480SC expressed as high potential chemical to control population of gram pod borer i.e. only 0.63 larvae/mrl amongst all modules. As result Flubendiamide takes lead role or being superior over other treatments. Rest of treatments also had significant control on gram pod borer followed by Indoxacarb 14.5SC (0.87 larvae/mrl), Spinosad 45SC (1.03 larvae/mrl), HaNPV + Btk (1.18 Larvae mrl), Novaluron 10EC (1.21 Larvae per meter row) at last the least effective or low potential was HaNPV 250LE (1.10 larvae/mrl). Regarding with grain yield also Flubendiamide 480SC proved its effectiveness by recording highest yield amongst all modules (i.e. 20-22 qt/ha).
Relationship between Profile of Rainfed Cotton Growers and Perception of Contingency Crop
Authors: Deshmukh Jyoti , Bhandarwar Vaishali
Published: December 2023

A contingency plan is a strategy that, intended to achieve a different result than what is typically or predictably anticipated. To put it another way, it is usually applied to risk management when a particularly high risk is present. The present study was conducted in Nanded district of Maharashtra and primary data was collected from 120 respondents by following personal interview method. The purpose of the present investigation is to focus on the relationship between the profile of cotton growers and perception. It was found that, annual income and land ownership, social participation, extension contact, cropping pattern and knowledge also had positive and significant relationships with perception of contingency crop plan. However, farming experience, education, family size and risk orientation had positive and highly significant relationships with perception of contingency crop plan.
Relationship between Some Kinematic Parameters of Ram Semen and Reproductive Performance of Dairy Sheep after application of assisted Reproductive Technologies
Authors: Sinapov Branimir , Yotov Stanimir
Published: December 2023

Aim of this study was to investigate the relationship between some kinematic parameters of ram semen and reproductive performance of dairy sheep after application of assisted reproductive technologies. The experiment was carried out with8 rams and 325 sheep from East Friesian breed and 7 rams and 162 sheep from Asaaf breed. Semen was collected by artificial vagina and subjected to primary assessment. Kinematic sperm parameters TM, PM, NPM, IS, VAP, VCL, VSL, LIN and STR were evaluated by computer-assisted semen analysis (CASA). All sheep were subjected to estrus synchronization by intravaginal sponges and artificially inseminated byfresh-diluted semen. Reproductive performance was recorded on the base of pregnancy rate (PR) and average litter size (ALS) calculated after lambing. Relationships between sperm kinematic parameters and these with the reproductive prformance were determined by correlation analysis. Significant differences between the mean values of age, body weight, ejaculate volume and sperm concentrationin the rams, and PR and ALS were not found. The sperm kinematic parametersshowed variability between individual rams. Total motility correlated positively with PM and LIN andnegativelywith NPM and IS (P<0.05). The correlation of PM with LIN and STR was positive, while this with NPM, IM and VCL was negative (P<0.05). A negative relationshipwas found of NPP and IS with STR and between LIN and VCL(P<0.05). The relationship of PM, VAP, LIN, STR with PR, and of LIN and STR with ALS (P<0.05) was positive, whereas this between VCL with PR was negative (P<0.05). In conclusion, the evaluation of the kinematic parameters by CASA is useful diagnostic and prognostic tool in sheep reproduction. The parameters PM, VAP, LIN and STR have a strong positive relationship (P<0.05) with the functional activity of ram spermatozoa and the reproductive performance of the sheep. They could be included as indicators in evaluation of the fresh semen quality in a selection of East Friesian and Asaaf rams with high fertility.
Analytic hierarchy process for evaluating the quality of courier services in e-commerce services from the perspective of Pos Indonesia Purwokerto
Authors: Gossa Jihad Sindhu , Ngafidin Khairun Nisa Meiah , Safitri Sisilia Thya
Published: December 2023

E-commerce became one of the technological developments in Indonesia in the fourth industrial revolution. Since then, the daily needs transaction process has become accessible through the e-commerce platform. The rapid development of e-commerce is predicted to increase and thus encourage the development of courier services over the past period, making courier services an essential element that affects customer satisfaction in e-commerce services. An analysis of customer satisfaction in several e-commerce service providers towards customer reviews on Google Play conducted by Sasmita and a statement by the Ministry of Trade through a consumer complaint report in 2021 stated that complaints about problems in the e-commerce sector have increased, which includes courier services incorporated with e-commerce service providers. Gulc argued that at least seven factors determine the quality of courier service based on the customer's perspective. The AHP method assists courier services affiliated with e-commerce services in recognizing which of these seven factors of courier service quality dimensions require attention to create a sustainable competitive advantage. This research aims to measure the priority level of criteria in determining the quality of courier services on e-commerce platforms, thus providing recommendations for the appropriate service priorities for courier service companies. The calculation results using AHP on the seven factors determining the quality of courier service according to the Manager Assistant of the Service Department at the Central Purwokerto Branch of the Kantor POS Indonesia show that the responsiveness criterion is more important than the other six criteria.
Genetic algorithm for finding shortest path of mobile robot in various static environments
Authors: Lestari Dyah , Sendari Siti , Zaeni Ilham Ari Elbaith
Published: December 2023

In conducting their work in the industry quickly, precisely, and safely, mobile robots must be able to determine the position and direction of movement in their work environment. Several algorithms have been developed to solve maze rooms, however, when the room is huge with several obstacles which could be re-placed in other parts of the room, determining the path for a mobile robot will be difficult. This can be done by mapping the work environment and determining the position of the robot so that the robot has good path planning to get the optimal path. In this research, a Genetic Algorithm (GA) will be used to determine the fastest route that a robot may take when moving from one location to another. The method used is to design a mobile robot work environment, design genetic algorithm steps, create software for simulation, test the algorithm in 6 variations of the work environment, and analyze the test results. The genetic algorithm can determine the shortest path with 93% completeness among the 6 possible combinations of the start point, target point, and position of obstacles. The proposed GA, it can be argued, can be used to locate the shortest path in a warehouse with different start and end points.
Scalable modular massive MIMO antenna of rectangular truncated corner patch antenna and circular slotted X patch antenna for 5G antenna communication
Authors: Salsabila Salwa , Pudjiastuti Rina , Nur Levy Olivia , Ryanu Harfan Hian , Nugroho Bambang Setia
Published: December 2023

Massive MIMO Antenna Design results in a very large antenna size that hinders the design process. The arrangement of Massive MIMO Antennas which consists of many antenna elements is a challenge in the design process due to the limited capability of the simulation software and the complicated process. Thus, a scalability technique is used to predict the specification results produced by a Massive MIMO Antenna array with a certain configuration based on a simple MIMO Antenna array with a 2x2, 4x4, 8x8, 16x16 MIMO element configuration scheme, etc. exponential increments. This research will discuss the scaling process to predict the specifications of a Massive MIMO Antenna array. The designed MIMO antenna arrangement is based on the design of a rectangular antenna with a truncated corner and a circular antenna with an X slot for further design with various types of configurations that work at a frequency of 3.5 GHz.
An enhancement to the FLC-based baby incubator system using genetic algorithm
Authors: Budiyanto Setiyo , Silalahi Lukman Medriavin , Gunawan Dadang , Triblas Adesta Erry Yulian
Published: December 2023

This research problem focuses on treating premature babies due to hypothermia so that the baby must be put in an incubator for several days. Conventional intensive care method in premature babies, namely skin-to-skin care method between mother and child. Meanwhile, the latest technological developments, the method is already based on electrical-Internet of Things (IoT) engineering. This research proposes the design of an IoT-based prototype known as a smart incubator. This prototype has been equipped with a real-time monitoring system and system settings using the mamdani fuzzy inference system control method and combined using the Genetic Algorithm (GA) method. The results showed that the ideal temperature range in the smart incubator was 33° C with an accuracy of 99.97% and was in accordance with the fuzzy membership degree in the range of 29° C ≤x≤ 37° C. Furthermore, the ideal relative humidity range in the smart incubator was 60% with an accuracy of 98.60% and was in accordance with the fuzzy membership degree in the range of 59 ≤x≤ 65. Then, the noise range in the smart incubator is 37.9dB to 56.8dB with an accuracy of 96.44% and has been appropriate at the fuzzy membership degree. At a maximum distance of 50cm, it takes 8 seconds for the prototype to detect movement as a safety measure.
Exploring early core symptoms in post-hepatectomy patients: a longitudinal study
Authors: Zhang Haoran , Li Shaoxue , Yuan Mengmeng , Liu Jingya , Fan Hua , Li Shuwen
Published: December 2023

Background: Patients with primary liver cancer (PLC) experience a range of symptoms in the early postoperative period. Symptoms include cancer-related symptoms and adverse effects of treatment. Exploring the core symptoms and their dynamics in the early post-hepatectomy patientsmay help provide better symptom management programs.
Objective: The purpose of this study was to identify the core symptoms in early post-hepatectomy patients and to explore the trajectory of their dynamics.
Methods: 249 participants with PLC underwent curative surgical procedures between March 2021 and September 2022 at the Hepatobiliary Surgery Departments of two Eastern Chinese hospitals. A comprehensive symptom assessment was administered to the patients 1-2 days after surgery (T1) and 1-2 days before discharge (T2). Network analysis was used to identify core symptoms in early post-hepatectomy patients based on symptom severity.
Results: The network analysis identified the core symptoms of dry mouth, pain, abdominal distension, lack of appetite, and nausea at T1. In contrast, at T2, lack of appetite, pain, shortness of breath, and disturbed sleep were revealed. At the two early time points, while the results of the network structure test showed no statistically significant difference in the overall symptom network structure, there was a significant difference in the results for the core symptoms.
Conclusion: Our study demonstrates the need to identify core symptoms in the early in early post-hepatectomy to improve symptom management in PLC patients. It is crucial to apply network analysis as an essential component of cancer care.
HIIT discontinuation may be detrimental to diabesity parameters whereas retraining enhances intestinal health and gut microbiota diversity
Authors: Ribeiro Filipe M. , Petriz Bernardo , Anderson Maycon , Assis Victoria , Rosa Thiago , Correa Hugo , Cavichiolli Nathalia , Passos Lana , Fonseca Amanda , Arnaldo Luiz , Silva Osmar N. , Castro Alinne , Franco Octávio L.
Published: December 2023

Background The term "diabesity," which refers to the simultaneous presence of diabetes and obesity, is associated with a significant mortality rate globally. However, there is a lack of comprehensive literature on the effects of stopping high-intensity interval training (HIIT) and then resuming it. This study sought to examine how the interruption and subsequent resumption of HIIT impacted the physiological changes in mice with diabesity. Seventy-five C57BL6 mice were used in this study, divided into five stages: baseline, diabesity induction, training, detraining, and retraining. Diabesity was induced in two groups of mice (obese - Western Diet, and control - diet AIN) over 10 weeks. Both groups were further divided into control and trained animals, with each stage (training, detraining, and retraining) lasting 6 weeks. HIIT was performed three times a week, consisting of five sets at 90% of maximum speed (Vmax). This study also analyzed various parameters, including lipid profile, glycemic kinetics, blood glucose, adiposity index, intestinal histology, and gut microbiota profile. Results HIIT resulted in metabolic enhancements and a fat loss, whereas the detraining led to a decline in these alterations. Although retraining exhibits a degree of blood glucose regulation, it was not effective in generating fat loss and weight loss. Conversely, HIIT retraining seems to enhance goblet cell populations and increase gut crypt depth, concurrently causing modifications in the gut microbiota composition. While both the initial training and subsequent retraining exerted an effect on the composition of the gut microbiota, the impact of a Western diet demonstrates a more substantial potency compared to that of physical training concerning intestinal health and microbial composition. Conclusions These findings may contribute to a broader understanding of diabesity management and introduce novel perspectives for the use of physical training to enhance patient outcomes in gut microbiota composition.
Effect of Endothelium on the Anticoagulant Activity of a Covalent Antithrombin-Heparin Complex
Authors: Atkinson Helen M. , Stevic Ivan , Berry Leslie R. , Chan Anthony K. C.
Published: December 2023

We have developed a covalent antithrombin-heparin complex (ATH) with superior anticoagulant properties compared to non-covalent antithrombin (AT) + unfractionated heparin (H). Previous in vitro investigations of ATH activity were done in the absence of endothelium. Since the endothelial surface can modulate hemostasis, we investigated the impact of endothelium on the anticoagulant capabilities of ATH and AT + H. Discontinuous second order rate constant (k2) inhibition assays of IIa and Xa by ATH and AT + H were performed in the presence and absence of endothelium. The effects of ATH and AT + H on IIa-initiated fibrin formation and plasma clot generation were also evaluated with and without endothelium present. ATH had an increased rate of direct inhibition of IIa and Xa compared to AT + H, and prevented IIa-induced fibrin formation to a greater degree. When compared at equal anti-Xa levels, ATH was less effective than AT + H at catalyzing inhibition of plasma clot generation. The presence of endothelium decreased the rate of IIa inhibition, and reduced clot time in IIa-induced fibrin formation and plasma clot generation assays. However, ATH maintained its relative efficacy compared to AT + H when endothelium was present. This supports the growing body of evidence suggesting ATH may be a beneficial anticoagulant for potential clinical use.
The usefulness of left and right ventricular GLS assessment and biomarker concentrations in cardiovascular risk stratification in patients qualified for hematopoietic stem cell transplantation due to hematopoietic and lymphoid malignancies – a pilot study description
Authors: Puła Bartosz , Kępski Jarosław , Misiewicz-Krzemińska Irena , Szmit Sebastian
Published: December 2023

Due to the cytostatic agents used and the risks associated with peri-transplant procedures, the hematopoietic stem cell transplantation (HSCT) procedure is considered a cardiovascular burden. We designed a pilot study to determine the clinical utility of the new ST2 marker and routinely assessed cardiac parameters in HSCT recipients. Based on the previous cardio-oncology experience in lung and prostate cancer, we can confirm the prognostic and predictive value of classic cardiac biomarkers and modern echocardiography parameters such as global longitudinal strain of the left and right ventricle. After conducting this pilot study, we can create a predictive and prognostic model for patients undergoing HSCT. This will greatly enrich our clinical practice, especially in treating older people.
Event-related potentials to single-letters and second-language reading proficiency
Authors: Takai Osamu , Small Jeff , Herdman Anthony T.
Published: December 2023

Proficient reading in a second language (L2) requires efficient processing of its orthographic symbols. We examined the relationship between a reader’s proficiency in English and electrophysiological markers of efficiency while processing English and Chinese orthographies. We recruited 49 adult late Chinese learners of English, who participated in an implicit single-letter processing task (alphabet letters vs. pseudoletters) and Chinese character processing task (characters vs. pseudocharacters) while an electroencephalogram measured their evoked potentials. The difference between letters and pseudoletters was manifested in the N170 and P2 ranges in a posterior-occipital distribution. The N170 pseudoletter effect in the left hemisphere correlated with L2 proficiency. We concluded that single-letter processing in L2 is still gaining efficiency as they learn to read English as a late-learn language. Future cross-linguistic investigations are necessary to determine whether this L2 effect generalizes to other L2 populations.
Genome sequencing analysis of the endophytic fungus Aspergillus tamarii TPD11
Authors: Zhang Qing , Liu Hai , Zhao Xiaojun , Yang Jili , Tang Weidi , Yang Ying , Chang Sheng , Cai Bo , Liu Juan , Zhu Yaoshun , Zhou Bo , Liu Tao
Published: December 2023

Background
The excavation and utilization of endophytic fungi from medicinal plants is of great importance for the development of new drugs. The endophytic fungus Aspergillus tamarii TPD11, which was isolated and obtained by the authors in the previous stage, can produce a variety of polyphyllin with important potential applications in hemostasis, inflammation and antitumor, however, the genomic information of TPD11 is still unknown.
Results
In this study, we sequenced and assembled the whole genome of the endophytic fungus A. tamarii TPD11, resolved the genomes of 24 Aspergillus for relationships, and phylogenetic analysis of the genomes of 16 strains revealed the evolutionary differences between Aspergillus and Penicillium and the mechanisms of genome expansion and contraction. CAZy annotation analysis showed that TPD11 obtains nutrients mainly by ingesting starch from the host plant. TPD11 has a biosynthetic gene cluster for the synthesis of squalestatin S1, and the silencing of this biosynthetic gene cluster might have a positive effect on increasing the content of polyphyllin. Annotation to 11 UDP-glycosyltransferase genes helps to further reveal the biosynthetic pathway of polyphyllin. In addition, secondary metabolism gene clusters and CAZy analysis confirmed the potential pro-biotic, insecticidal and antimicrobial activities of TPD11 on host plants.
Conclusions
This study reveals the intrinsic mechanism of endophytic fungi to promote the content of polyphyllin in paris, which provides a basis for the synthetic synthesis of the natural product polyphyllin.
Identification of differentially expressed genes and hub genes of human hosts with tuberculosis through an integrated bioinformatics and cell confirmation strategy
Authors: Yue Peng , Dong Yan , Ma Weijie , Xu Xin , Kong Jing , Chen Jingjing , Fan Yuxin , Liu Meixiao , Cao Wenjing , Wen Shiyuan , Li Binxue , Luo Lisha , Chen Taigui , Li Lianbao , Liu Aihua , Bao Fukai
Published: December 2023

Tuberculosis is a chronic infectious disease caused byM.tuberculosis. The immune defence mechanism of the body against tuberculosis is still unclear. We used four microarray datasets from the Gene Expression Omnibus (GEO) to identify differentially expressed genes (DEGs) between samples from humans infected withM.tuberculosisand healthy controls group. Then, the host hub genes with a relatively high number of connections to other DEGs were identified by Cytoscape. Other bioinformatics methods are also performed, including protein–protein interaction (PPI) network analysis and construction of miRNA–hub gene networks and transcription factors (TF)–hub gene networks. Finally, the expression of hub genes in macrophage infected byM.tuberculosiswas verified using the reverse transcription polymerase chain reaction (RT–PCR). A total of 46 DEGs were identified. Gene Ontology (GO) analysis showed that the biological functions of DEGs. Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analysis showed involvement of the genes in the NOD-like receptor and toll-like receptor signaling pathways. Five highly differentially expressed hub genes (STAT1, TLR7, CXCL8, CCR2, and CCL20) were identified. Finally, based on NetworkAnalyst's database, we constructed miRNA–hub gene networks and TF–hub gene networks.
Long-Term Effects of COVID-19 on School-Aged Adolescents in Iraq
Authors: Sadiq Alaa M. , Al-Amran Fadhil
Published: December 2023

Background:This study investigates the long-term effects of COVID-19 on Iraqi adolescents aged 12-18, who are free from chronic conditions, across various provinces.Methods and Findings: A cohort of 2000 students was evaluated through physical, psychological, and academic assessments. The study revealed a significant presence of long-COVID symptoms, including fatigue and cognitive disturbances, across the cohort. Mental health assessments indicated heightened levels of anxiety and stress. Furthermore, a noticeable decline in academic performance was observed, particularly in regions with limited access to digital learning tools. These results highlight the multi-faceted impact of the pandemic on adolescents.Conclusions: The findings emphasize the need for holistic approaches that encompass health monitoring, mental health services, and educational support to mitigate the lingering effects of the pandemic on young individuals. Region-specific strategies are essential to address the diverse challenges faced by adolescents in different Iraqi provinces.
Rapid time-domain simulation of fractional capacitors with SPICE
Authors: Wilson Marcus , Cowie Logan , Farrow Vance , Cree Michael , Scott Jonathan
Published: December 2023

Fractional Capacitors, commonly called Constant-Phase Elements or CPEs, are used in modeling and control applications,for example for rechargable batteries. Unfortunately, they arenot natively supported in the well-used circuit simulator SPICE.This manuscript presents and demonstrates a modeling approach that allows users to incorporate these elements in circuits and model the response in the time domain. We use an array of RC elements in parallel to construct acircuit with SPICE that shows constant phase behavior across a definedfrequency range. We demonstrate that the circuit produces the required impedance spectrum in the frequency domain, and shows a power-law voltage response to a step change in current in the time domain, consistent with theory,and is able to reproduce the experimental voltage response to a complicated current profile in the time domain. The error dependson the chosen frequency limits and the number of RC branches, inaddition to very small SPICE numerical errors. We are able to define an optimum circuit description that minimizes error whilemaintaining a short computation time. The work allows a rapid and accurate evaluation of the response of CPEsin the time domain.
Phytotoxicity and plant uptake of decabromodiphenyl ethane under citric acid
Authors: 刘 瑞源 , Xie Hui
Published: December 2023

The novel brominated flame retardant decabromodiphenylethane (DBDPE) has biological toxicity, persistence, long-range migration and bioaccumulation ability. However, there is currently a lack of research on the phytotoxicity of DBDPE to herbaceous plants. Therefore, this study selected the perennial herbaceous plant tall fescue as the model material and conducted outdoor seed germination experiments using soil contamination method to study the toxic effects of DBDPE on tall fescue seedlings. The results indicated that DBDPE significantly inhibited the germination of tall fescue seeds, and it had significant impact on the physiological and biochemical indicators and antioxidant enzyme activity of tall fescue seedlings. Citric acid could effectively alleviate the toxicity of DBDPE to tall fescue, and seedlings improved their tolerance to DBDPE stress by regulating the content of proline, soluble protein, and soluble sugar in cells. Compared with the control, there was no significant difference in superoxide dismutase (SOD) and peroxidase (POD) activities in tall fescue seedlings, but catalase (CAT) activity and malondialdehyde (MDA) content were significantly increased. Tall fescue seedlings had certain bioaccumulation effect for DBDPE, with biological concentration factor (BCF) ranging from 4.28 to 18.38 and transfer factor (TF) ranging from 0.43 to 0.54, and DBDPE was mainly bio accumulated in the roots of tall fescue seedlings. The citric acid significantly promoted the bio concentration capacity of tall fescue seedlings for DBDPE. It provides theoretical support for the toxicity study of DBDPE on plants, and it provides certain research foundation for exploring the phytoremediation of DBDPE contaminated soil by tall fescue.
EFFECTS OF PHYSICAL AND COGNITIVE FACTORS ON REACTIVE AGILITY IN PROFESSIONAL FOOTBALL PLAYERS
Authors: Siyah Mustafa , Şanlı Tuğba , Turgut Elif
Published: December 2023

Amaç: Bu çalışmanın birincil amacı futbolcularda fiziksel ve kognitif faktörlerin reaktif çeviklik üzerine etkisini incelemek; ikincil amacı ise futbolda alt ekstremite yaralanma riski düşük olan ve yüksek olan futbolcularda reaktif çeviklik parametrelerini karşılaştırmaktır.
Yöntem: Çalışmaya 30 profesyonel futbol oyuncusu dahil edildi. Tüm katılımcılara fiziksel ve kognitif değerlendirmeler uygulandı. Reaktif çeviklik değerlendirmesinde görsel uyaran ve fotoselli kapılar içeren Reaktif Çeviklik Testi (RÇT) kullanıldı. Tablet temelli sağ/sol diskriminasyonu, Dikey Sıçrama Testi, T Çeviklik Testi (T-Test), Y Denge Testi (Y-Denge), 20 m Sprint Testi ve Hamstring Eksentrik Kuvveti değerlendirildi. Yaralanma risk analizi için ise Tuck Jump Testi kullanıldı.
Sonuçlar: Çalışmamızda RÇT hareket süresi parametresi ile Y-Denge testi komposit, posteromedial ve posterolateral uzanma skoru ve sağ/sol diskriminasyonu doğruluk oranı arasında negatif yönde; T-Test ile pozitif yönde bir ilişki vardı (p
HOW PILATES EXERCISES AFFECT SPORTS PERFORMANCE? A SYSTEMATIC REVIEW
Authors: Yılmaz Osman , Soylu Yusuf , Kaplan Turgut , Taşkın Mine
Published: December 2023

Purpose: This systematic review aims to describe and summarise the effects of Pilates exercises (PE) on athletic performance in athletes.
Methods: The published literature was reviewed in the electronic databases of PubMed, Web of Science, and SCOPUS between 01.01.2010 and 01.01.2022. The articles were determined according to the PICO criteria (population, application, comparison, and results) using the keywords "pilates training", "pilates exercises", and "pilates and athlete performance" in the title and abstract sections. The titles and abstracts of articles were screened for relevance to the main research topic of this study.
Results: A total of 151 articles were identified from the database search. Twelve articles were included in the systematic review. The results showed that the studies carried out PE practice between four weeks and 14 weeks. Also, it was found that, PE helps to improve postural stability, static and dynamic balance, agility, muscle strength, flexibility, core strength, muscular endurance, general sprint performance, VO2 max level, coordination, and technical skills in athletes.
Conclusion: Coaches and sports performance specialists can help athletes improve their physical performance and core strength levels by including PE in their training programmes.
To Investigate Growth Factor Receptor Targets and Generate Cancer
Targeting Inhibitors
Authors: Basu Debroop , Pal Riya , Sarkar Maitrayee , Barma Soubhik , Halder Sumit , Roy Harekrishna , Nandi Sisir , Samadder Asmita
Published: December 2023

Receptor tyrosine kinase (RTK) regulates multiple pathways, including Mitogen-activated protein kinases (MAPKs), PI3/AKT, JAK/STAT pathway, etc. which has a significant role in the progression and metastasis of tumor. As RTK activation regulates numerous essential bodily processes, including cell proliferation and division, RTK dysregulation has been identified in many types of cancers. Targeting RTK is a significant challenge in cancer due to the abnormal upregulation and downregulation of RTK receptors subfamily EGFR, FGFR, PDGFR, VEGFR, and HGFR in the progression of cancer, which is governed by multiple RTK receptor signalling pathways and impacts treatment response and disease progression. In this review, an extensive focus has been carried out on the normal and abnormal signalling pathways of EGFR, FGFR, PDGFR, VEGFR, and HGFR and their association with cancer initiation and progression. These are explored as potential therapeutic cancer targets and therefore, the inhibitors were evaluated alone and merged with additional therapies in clinical trials aimed at combating global cancer.
Frequency Distribution of Depression and its Associated Factors among Pregnant Women during the COVID-19 Pandemic
Authors: Abbasi Zahra , Saghari Sam , Nashtifani Abdorrahman Harif , Daneshi Salman , Hushmandi Kiavash , Raesi Rasoul
Published: December 2023

Aim:

This study aimed to determine the frequency distribution of depression and its associated factors among pregnant women.

Background:

Health is mentioned as a prerequisite for sustainable development in every society, and pregnant women play an irreplaceable role as the central axis of the health of society.

Methods:

A cross-sectional study was conducted with 102 pregnant women living in northeastern Iran, in 2021. The data was collected using an electronic questionnaire that included demographic questions and the Beck Depression Inventory items. Data analysis was done using SPSS-22 statistical software and independent t-tests, one-way analysis of variance, Tukey's post hoc, and linear regression. A significance level of 0.05 was considered.

Results:

The mean depression score of pregnant women was 25.77 ± 6.62, with the majority (53.9%) suffering from moderate depression. The frequency distribution of depression was lower among university-educated women than those with lower-level degrees and among employed women than homemakers (P <0.05). The third trimester of pregnancy was associated with an increased risk of depression (P <0.001). The frequency distribution of depression increased with increasing numbers of children (P <0.001). Miscarriage, a family history of depression, an unwanted pregnancy, and the infliction of close relatives with COVID-19 all increased the risk of depression in women (P <0.05).

Conclusion:

Pregnancy during COVID-19 pandemic can be associated with potential complications for the embryo, mother, and child. Amid the current global COVID-19 Pandemic, all pregnant women must undergo routine screening for depression as part of their prenatal care.
The Maternal Outcome of Patients with COVID-19 and their babies in the first 24 hours of Birth
Authors: Salari Saeideh , Raesi Rasoul , Teimoori Niloofar , Jafarnejad AbuBakr , Daneshi Salman
Published: December 2023

Aim:

This study investigates the maternal outcome of patients with COVID-19 and their babies in the first 24 hours of birth in 2021 in Ayatollah Kashani Hospital, Jiroft.

Background:

The widespread spread of Covid-19 was accompanied by concern about vulnerable groups of society such as pregnant women and infants. This group of people in society is susceptible to this disease due to the weakening of the immune system.

Methods:

This cross-sectional study was conducted on 81 pregnant women with COVID-19 in 2021 in Ayatollah Kashani Jiroft Hospital. The data collection tool was adjusted according to the maternal and neonatal outcomes, and the inclusion criteria included pregnancy over 34 weeks confirmed by ultrasound and infection with COVID-19 confirmed by PCR test. Data were collected using a checklist and maternal and neonatal outcomes were reported. Data were analyzed with SPSS version 25 software and the significance level was ≤ 0.05.

Results:

The prevalence of death in mothers with COVID-19 was 3.7%, in the present study, there was no higher rate of cesarean delivery, and 14.8% had premature delivery. The Apgar score of the examined infants at minute 0 was higher than 7, and infant death was not found, 18.5% were transferred to ICU and 29.6% had lung involvement.

Conclusion:

The results of this research showed that maternal complications, especially maternal death were more than expected but symptoms in infants did not significantly increase and infant death was not found in this study. However, it should be noted that the complications of COVID-19 in infants can be serious.