bluuebunny/crossref_metadata_2025 huggingface dataset:
Rat polyvinyl sponge model for the study of infections: initial investigations
Authors: Isenberg H D , Wiener S L , Isenberg G A , Sampson-Scherer J , Urivetzky M , Berkman J I
Published: January 2020

Polyvinyl sponges were implanted subcutaneously on both sides of young female rats. One sponge was infected with 10(8) of either Escherichia coli K-12 F-, Staphylococcus aureus ATCC 25923, or Pseudomonas aeruginosa CDC 7725. P. aeruginosa remained at the inoculum level and S. aureus declined by 1 log, whereas E. coli was reduced 1,000-fold. Only P. aeruginosa was recovered from the blood in 36% of the animals in 24 h and in 20% of the rats in 48 h. The nutrient potential of rat inflammatory fluid was compared to nutrient broth by growth of each bacterium in untreated and heat-inactivated sponge fluids and Trypticase soy broth.
Peroxidase staining in elicited and nonelicited mononuclear peritoneal cells from BCG-sensitized and nonsensitized mice
Authors: Lepper A W , Hart P D
Published: January 2020

The peroxidase (PO) activity in nonelicited macrophages and in casein-elicited monocytes, obtained from peritoneal cavities of nonsensitized and BCG-sensitized mice and cultivated on glass for 1 or 2 h, was studied by light and electron microscopy, using the 3,3'-diaminobenzidine technique. These two types of glass-adherent peritoneal cells differed in PO activity. In macrophages, PO activity was predominantly in the nuclear envelope, rough endoplasmic reticulum, and occasionally in vesicles of the Golgi apparatus. In monocytes, PO activity was confined to cytoplasmic dense bodies resembling lysosomes, and was greater at 10 and 24 h after elicitation than at 96 h. The BCG sensitization did not significantly alter the proportion of cells with PO-positive granules in macrophages or monocytes from that observed in nonsensitized mice. From its lysosomal site, the PO in monocytes could come into contact with those microorganisms whose ingestion by these cells was followed by phagolysosome formation.
Two previously undescribed extracellular streptococcal antigens detectable with naturally occurring human antibodies
Authors: Kiefer D , Halbert S P
Published: January 2020

Two streptococcal extracellular antigens (X and Y), which are detectable with naturally occurring human antibodies, have been isolated and shown to represent entities distinct from previously described streptococcal enzymes, toxins, and antigens. They are each synthesized by both group C and group A streptococci and appear to be proteins. Both antigens were found to be nonhemolytic and nontoxic for human leucocytes as well as for isolated beating newborn rat heart cells in tissue culture. In addition to being distinct from known streptococcal enzymes, they were also shown to be devoid of several other enzymatic activities.
Assay of bovine interferons in cultures of the porcine cell line IB-RS-2
Authors: Ahl R , Rump A
Published: January 2020

An assay for bovine interferons has been developed using the porcine cell line IB-RS-2 and a bovine enterovirus, CBV-D, as challenge virus. The method is based on estimation of cytopathic effect measured by uptake of neutral red. Teh assay is simple, sensitive, and reproducible. A comparative test of different viruses in IB-RS-2 cells and secondary calf kidney cells revealed that the sensitivity of a virus to interferon can vary up to 1,000-fold in the two cell systems. Vesicular stomatitis virus was found to be rather insensitive to interferon in IB-RS-2 cells.
Fetal and adult bovine interferon production during bovine viral diarrhea virus infection
Authors: Rinaldo C R , Isackson D W , Overall J C , Glasgow L A , Brown T T , Bistner S I , Gillespie J H , Scott F W
Published: January 2020

Levels of interferon in adult bovine serum and in fetal bovine serum and tissues were examined during the course of transplacental bovine viral diarrhea virus infection. The cows produced circulating interferon between 2 and 9 days after viral inoculation, with mean peak levels in the serum on day 4. Interferon could be routinely detected in fetal tissues (e.g., thymus, spleen, and kidney) between days 4 and 21 after viral inoculation of the cows at 149 to 150 days of gestation (mid-second trimester) and in fetal serum from day 13 through day 21. Interferon was also detectable in the serum and tissues of fetuses from dams infected at day 95 of gestation (the beginning of the second trimester). In general, no differences were found between the ability of the adult and fetus to produce interferon. Fetal lamb kidney cells were more sensitive to the antiviral effects of bovine interferon than were fetal bovine kidney cells. The antiviral substance from the fetal and adult animals was characterized as interferon by standard criteria.
Morphology and time course of experimental listeriosis in nude mice
Authors: Heymer B , Hof H , Emmerling P , Finger H
Published: January 2020

Experimental listeriosis in phenotypically normal (nu/+) euthymic NMRI mice has a characteristic morphology and short-term course. In contrast, listeric infection in congenitally dysthymic nude (nu/nu) mice does not proceed in clear-cut phases, develops more slowly, displays a chronic tendency from the beginning, and shows a considerably different morphology. The inability of nude mice to effectively control and terminate infection by Listeria monocytogenes obviously results from the lack of T lymphocytes.
Pinocytic rates of macrophages from mice immunized against Toxoplasma gondii and macrophages stimulated to inhibit toxoplasma in vitro
Authors: Jones T C , Len L
Published: January 2020

The rate of pinocytosis by macrophages when measured by uptake of horseradish peroxidase was significantly reduced during toxoplasma infection of the cells in vitro when the macrophages were from toxoplasma-immune mice and when control cells were stimulated in vitro to inhibit toxoplasma multiplication. There was, however, no direct correlation between reduced pinocytosis in this model and inhibition or enhancement of toxoplasma multiplication. We conclude that a reduced pinocytic rate is a feature of the unstimulated toxoplasma-immune macrophage, but this change in rate alone does not correlate with the cell's ability to inhibit toxoplasma. In addition, we observed that enhanced pinocytosis as seen in the elicited macrophage was not a requirement for inhibition of toxoplasma multiplication.
Lymphocyte and macrophage responses after vaccinia virus infections
Authors: McLaren C , Cheng H , Spicer D L , Tompkins W A
Published: January 2020

Using a semimicromethod with washed whole blood, in vitro lymphocyte responses of rabbits to intradermal infection with vaccinia virus was studied. Peritoneal exudate macrophages were infected with vaccinia in vitro to determine the time of appearance of activated macrophages. After primary infection, an increase in spontaneous incorporation of thymidine by blood cultures was found as early as 2 days postinfection. This effect was at a maximum at 7 to 10 days, with counts up to 100-fold higher than before infection. Incubation of these cultures with concanavalin A showed a marked decrease in stimulation index as compared with normals. Although only a transient stimulation with vaccinia was found during the acute infection, stimulation indexes of 2 to 3 were obtained during convalescence. Macrophages from rabbits early after infection supported vaccinia replication, whereas those at day 6 or later resisted infection. Macrophage resistance persisted for 2 to 3 weeks. The response of lymphocytes from rabbits reinfected with vaccinia after 15 weeks differed, with a small increase in spontaneous thymidine uptake, a smaller depression in concanavalin A stimulation, and a greater specific response to vaccinia. Macrophage activation occurred earlier and persisted for a longer time after secondary infection.
Passive hemolysis test for antibody to Treponema hyodysenteriae
Authors: Jenkins E M , Sinha P P , Vance R T , Reese G L
Published: January 2020

Swine were infected orally with pure cultures of Treponema hyodysenteriae, and the humoral antibody response was measured by a passive hemolysis test (passive hemagglutination test with the use of complement). Antibody to T. hyodysenteriae was detected as early as 1 week and later on at 4 months after exposure. However, peak serum titers were obtained after challenge at 4 weeks postinfection. The usefulness of the test as a potential diagnostic tool for antibody to T. hyodysenteriae is discussed.
Effect of serum on phospholipids of L-forms of Streptococcus faecalis
Authors: Montgomerie J Z , Kaplan D , Schotz M , Guze L B
Published: January 2020

Changes in the phospholipids of the membrane of L-forms of Streptococcus faecalis were studied after exposure to normal human serum. The phospholipids were labeled by incubating the L forms with [14C]palmitate. After the reaction of the L-forms with normal human serum, the percentage of total lipid radioactivity decreased in phospholipids and increased in free fatty acids, indicating phospholipase activity. Similar changes were seen on reacting normal human serum with autoclaved L-forms. Sonication of the L-forms resulted in only a slight increase in phospholipase activity. These results suggested that a major part of the phospholipase activity was in the serum.
Intrauterine transmission of Sendai virus in inbred mouse strains
Authors: Tucker M J , Stewart R B
Published: January 2020

A study of Sendai virus infection in adult mice (2 to 3 months of age) showed that the inbred strains were more susceptible to infection than randomly bred Swiss white mice and that virus could be isolated from inbred strains for as long as 21 days postinfection. For this reason, these mouse strains (C57B1/6J [black] and C57Br [brown]) were selected for the study of intrauterine transmission of virus. The major effect of infection was a decreased weight of both embryos at 16 days of gestation and newborn mice. Virus was isolated from 17 to 20% of the embryos and at least 20 to 30% of the newborns from intravenously infected mothers. Fluorescent-antibody studies showed that the virus was widely distributed in the tissues of both embryos and newborns, including the central nervous system.
Activity in saline of phthalylated or succinylated derivatives of mycobacterial water-soluble adjuvant
Authors: Audibert F , Chedid L
Published: January 2020

A water-soluble fraction (WSA) of the cell wall can substitute for mycobacterial cells in Freund complete adjuvant. However, when WSA is administered in saline instead of in a water-in-oil emulsion, its adjuvant activity is very weak, and under certain experimental conditions it can even inhibit the humoral immune response. The data reported in the present study show that after treatment by phthalic or succinic anhydride the adjuvant activity of WSA was markedly changed, since high levels of circulating antibodies were produced when these derivatives were administered with an antigen in an aqueous medium. Moreover, the antigenic determinants of WSA were modified and acylated WSA had no tuberculin-like activity.
Immunological and serological diversity of Neisseria gonorrhoeae: gonococcal serotypes and their relationship with immunotypes
Authors: Wong K H , Arko R J , Logan L C , Bullard J C
Published: January 2020

Strains of gonococcus were shown to be immunologically heterologous. Serum bactericidal activity generally correlated with induced immunity to gonococcal challenge as detected by the guinea pig subcutaneous chamber model. Sera devoid of bactericidal activity reflected the lack of cross-protection in subcutaneous chambers. Factors affecting the bactericidal assay described in this report include (i) source of complement, (ii) concentration of test antigen and complement activity, and (iii) presence of calcium and magnesium ions and bovine serum albumin in diluent. Poor correlation was observed between agglutinating activity of the immune sera and protection.
Lung weight parallels disease severity in experimental coccidioidomycosis
Authors: Huppert M , Sun S H , Gleason-Jordon I , Vukovich K R
Published: January 2020

Evidence provided by histopathological study of lesions is a valuable adjunct for evaluating chemotherapeutic efficacy in experimental animal models, In addition, this should be correlated with a measure of disease severity in the same animal. The latter could be obtained by homogenization of infected organs and quantitative enumeration of viable cells of the etiological agent, but this would preclude histopathological studies in the same animal. Progression of disease in pulmonary infection is associated with replacement of air space by fluid, cells, and cellular debris. Therefore, an increase in lung weight should reflect severity of disease. Results with the murine model of coccidioidomycosis demonstrate that increasing lung weight parallels the increasing census of fungus cells in the lungs of both treated and nontreated infected mice. This was supported with evidence obtained from microscopic studies of lesions indicating that specific chemotherapy limited spread of the infection and inhibited multiplication of the fungus in the lung. Therefore, lung weight can be used as a measure of disease severity in the murine model of coccidioidomycosis.
Immunogenic activity of a ribosomal fraction obtained from Pasteurella multocida
Authors: Baba T
Published: January 2020

The cells of P. multocida strain P-1059 were destroyed with the French pressure cell; the ribosomal fraction proven to be homogeneous by analytical ultracentrifugation was obtained from the product by centrifugal fractionation, zonal electrophoresis, and Sephadex G-200 gel filtration. The ribosomal fraction exhibited intense protective antigenicity in mice and chickens, but the lipopolysaccharide (endotoxin) and the other bacterial cell fraction obtained in this experiment did not. Sodium deoxycholate treatment of the ribosomal fraction resulted in only a 13% loss in immunological activity, and ribonuclease treatment caused a 60% loss of activity.
Influence of temperature on opsonization and phagocytosis of staphylococci
Authors: Peterson P K , Verhoef J , Quie P G
Published: January 2020

The effect of incubation temperatures of 41, 37, and 4 degrees C on phagocytosis was investigated using human neutrophils and [3H]thymidine-labeled staphylococci. Depressed phagocytosis was observed at 41 and 4 degrees C. At 41 degrees C diminished staphylococcal uptake resulted from decreased attachment of bacteria to leukocytes; the inhibitory effect at 4 degrees C was secondary both to decreased opsonization and to reduced attachment to leukocytes. In contrast to the findings with normal serum, opsonization with heat-inactivated serum appeared to be relatively intact at 4 degrees C. By incubating samples in lysostaphin, it was determined that the process of bacterial ingestion as well as that of attachment was adversely affected by incubation temperatures of 41 and 4 degrees C.
Interferon induction by radioprotective mercaptoalkylamines and derived thiophosphates
Authors: Lvovsky E , Levy H B , Doherty D G , Baron S
Published: January 2020

Radioprotective thiols (five mercaptoalkylamines and four derived thiophosphates) induced interferon and resistance to virus infection. Interferon production occurred in human and mouse nonlymphoid cell cultures. One of the thiols, S,2-aminoethylisothiourea, given intraperitoneally, protected mice against two unrelated viruses--Semliki forest virus and Herpesvirus hominis type 1. Two structurally different radioprotective thiols--the disulfide cystamine and L-cysteine--were unable to induce the firus resistance state or interferon.
Distribution of Antibodies to Streptococcal Esterases in Patients with Scarlet Fever
Authors: Hayano Seiki , Tanaka Atsuko
Published: January 2020

Repetitive counterelectrophoresis (RCE), which has been described (Hayano and Tanaka, 1977), was used to assay the contents of antibodies to streptococcal esterases (STE) in sera from patients with scarlet fever. The levels of antibodies to STE were expressed semiquantitatively by reading the intensity of the colored spot developed by RCE with a densitometer. The present study deals with the determination of anti-STE in sera drawn at intervals from 54 patients diagnosed as suffering from scarlet fever. The STE used in this study were prepared from the streptococcal strains as follows. STE-AI was prepared from SS379 (group A, type 40), STE-AII from strain 69882 (group A, type 49), STE-B from strain H36B (group B, lb), and STE-C from strain Austin (group C). Of the 54 cases studied, 32 (59.3%) showed anti-STE-AI, 24 (44.4%) showed anti-STE-AII, 5 (9.3%) showed anti-STE-B, and 23 (42.6%) showed anti-STE-C. Comparison of the titer of anti-streptolysin O (ASLO) with the type of specific reaction of anti-STE-AI and -AII, determined in the same specimen, showed a marked correlation. Of 20 cases giving ASLO titers of 12 or less, 18 showed no sign of anti-STE-AI and -AII and two showed signs of anti-STE-AI. Of 34 cases giving ASLO titers of more than 12, 33 showed signs of anti-STE-AI and/or -AII, 22 showed signs of anti-STE-AI and -AII, 7 showed signs of anti-STE-AI, and 4 showed signs of anti-STE-AII. Of all the cases, 26 showed coincident rises in titers of ASLO and levels of anti-STE-AI and/or -AII during the course of the disease. One case showed the presence of only anti-STE-B. All of the 23 cases that showed the presence of anti-STE-C showed signs of anti-STE-AI. Some of these cases showed a marked increase in levels of anti-STE-C during the course of the disease.
Cell-mediated immunity in experimental Nocardia asteroides infection
Authors: Sundararaj T , Agarwal S C
Published: January 2020

Experimental mycetoma-like lesions developed in guinea pigs after subcutaneous injection of Nocardia asteroides. Although delayed hypersensitivity appeared earlier, increased macrophage migration inhibition and microbicidal activity appeared after 7 weeks. When the lesions healed, high cell-mediated immunity was present. Cell-mediated immunity was transferred to normal recipient guinea pigs from healed donor guinea pigs by spleen cell transfer. Recipient guinea pigs showed marked protection against challenge with N. asteroides.
Interaction of Treponema pallidum (Nichols strain) with cultured mammalian cells: effects of oxygen, reducing agents, serum supplements, and different cell types
Authors: Fitzgerald T J , Johnson R C , Sykes J A , Miller J N
Published: January 2020

Cultured mammalian cells extend the time of survival of Treponema pallidum (Nichols strain). Various parameters that have been previously shown to enhance treponemal survival in vitro were examined for influences on the interaction of T. pallidum with cultured cells. With cells derived from normal rabbit testes, the time of retention of treponemal virulence was extended in an atmosphere containing reduced concentrations of oxygen. Glutathione and cysteine, when added to the basal tissue culture medium, prolonged treponemal survival. In an assessment of various tissue culture medium supplements, normal rabbit serum was equivalent to fetal bovine serum and superior to bovine serum albumin fraction V (BSA), fatty acid-poor BSA, and lipid-pooed for TRK-2, HSE, NRK, and C6 cells. Dithiotreitol, as an additional reducing agent, sharply enhanced treponemal survival. With SF1Ep NBL-11 cells and basal tissue culture medium containing glutathione, cysteine, and dithiothreitol, in an atmosphere of approximately 3% oxygen, T. pallidum was maintained without detectable decreases in the number of virulent organisms for 6 days.
Lack of protection afforded by ribonucleic acid preparations from Mycobacterium tuberculosis against Mycobacterium leprae infections in mice
Authors: Shepard C C , Youmans A Y , Youmans G P
Published: January 2020

Mycobacterial ribonucleic acid preparations from H37Ra, an attenuated strain of Mycobacterium tuberculosis, provide their usual marked protection against M. tuberculosis challenge; however, they provided no protection against Mycobacterium leprae challenge. Suspensions of intact H37Ra were not effective against M. leprae. Suspensions of BCG gave their usual distinct protection against M. leprae challenge.
Experimental Bacteroides fragilis endocarditis in rabbits
Authors: Carrizosa J , Tanphaichitra D , Levison M E
Published: January 2020

A serum-resistant strain of Bacterioides fragilis that did not produce heparinase was used to study the characteristics of B. fragilis endocarditis in the rabbit experimental model. The infective dose required to produce endocarditis in 50% of rabbits was significantly lower for rabbits with left-sided intracardiac catheters (log10 6.3 colony-forming units +/- 0.6/ml) as compared with right-sided intracardiac catheters (log10 7.7 colony-forming units +/- 0.8/ml). After 3 days of infection, bacterial titers of the tricuspid vegetations were significantly lower than titers of aortic vegetations (P less than 0.01), although at 5 days the titers were similar (P greater than 0.05). The weights of tricuspid vegetations, although similar at 3 days (P less than 0.05). There were no spontaneous deaths during 12 days of infection. In rabbits with the catheter removed before infection, bacterial titers were similar to those titers in rabbits with the catheter continuously in place. This model will permit study of various drug regimens for treatment of this disease.
Solid-phase radioimmunoassay of human immunoglobulin M and immunoglobulin G antibodies against herpes simplex virus type 1 capsid, envelope, and excreted antigens
Authors: Kalimo K O , Marttila R J , Granfors K , Viljanen M K
Published: January 2020

A solid-phase radioimmunoassay developed in our laboratory for detection of human viral immunoglobulin M (IgM) and IgG antibodies was applied to demonstrate human class-specific antibody response against capsid, envelope, and excreted antigens of herpes simplex virus type 1. In primary infections, a clear IgM and IgG antibody response was found predominantly against the envelope components, whereas the IgM and IgG antibodies to the capsid antigen appeared more slowly. Increasing IgG antibody titers to the excreted antigen were also found in primary infections, though appearing more slowly than antibodies to the other subunit antigens. The antibody response against capsid and envelope antigens was not type specific, whereas in primary infections IgG class antibodies against the excreted antigen showed distinct type specificity. In recurrent infections, no significant level of IgM class antibodies was demonstrated, but in the patients with a severe secondary herpes simplex virus infection a definite IgM class antibody response was found against the envelope antigen. In addition, during severe secondary infections the antibody response against the excreted antigen was enhanced. The host IgG antibody response in recurrent infections was directed against the envelope and excreted antigens, whereas the level of the capsid antibodies was relatively stable.
Binding of Progesterone to
Neisseria gonorrhoeae
and Other Gram-Negative Bacteria
Authors: Miller Richard D. , Morse Stephen A.
Published: January 2020

The binding of [1,2-
3
H]progesterone to progesterone-sensitive
Neisseria gonorrhoeae
CS-7 and the progesterone-insensitive
Neisseria mucosa, Pseudomonas aeruginosa
, and
Salmonella typhimurium
(rough and smooth strains) was investigated. The kinetics of binding to
N. gonorrhoeae
CS-7 demonstrated that the majority of the progesterone binding occurred and equilibrium was reached within the first 30 min. Despite the rapid binding of progesterone, only about 20% of the added steroid was bound at the cell concentration used throughout this study. Whole cells of progesterone-insensitive bacteria bound progesterone less efficiently than the progesterone-sensitive
N. gonorrhoeae
CS-7.
N. mucosa
bound low amounts of this steroid (20% of that bound by
N. gonorrhoeae
CS-7) whereas the other gram-negative bacteria exhibited little progesterone binding (<3% of that bound by
N. gonorrhoeae
CS-7). The outer membrane permeability of
N. gonorrhoeae
CS-7, as measured by crystal violet uptake and inhibition, was similar to the deep rough mutant of
S. typhimurium
TA 1535. The latter organism neither bound nor was inhibited by progesterone. However, isolated cell envelopes of
N. gonorrhoeae
and progesterone-insensitive bacteria all bound progesterone equally well. Cortisone and cholesterol, althouh structurally similar to progesterone, were not inhibitory to
N. gonorrhoeae
and did not bind to whole cells as well as progesterone. The major site of progesterone binding appeared to be the cytoplasmic membrane, which bound four times more progesterone than the outer membrane. In addition, isolated cytoplasmic membrane proteins bound more than three times more progesterone per milligram of protein than the intact membrane.
Immunological Responsiveness to
Escherichia coli
During Pregnancy
Authors: Kenny Jean F. , Diamond Mark
Published: January 2020

To determine whether immunological responsiveness to a bacterial antigen is altered during pregnancy and lactation, Swiss mice (gestation, 19 to 21 days) were studied during early, middle, and late pregnancy and in the early postpartum period. Pregnant and nursing mice, each along with a virgin female littermate control, were injected with 2 × 10
6
heat-killed
Escherichia coli
and sacrificed 4 days later for the enumeration of splenic anti-
E. coli
plaque-forming cells (PFC). For 72 4- to 10-day pregnant and control mice ranked together, total PFC per spleen ranged from 0 to 312,650, with 61% of the counts from pregnant animals ranking above the median of 12,700. The mean number of PFC for the pregnant animals was greater than for sister controls in 19 of 23 litters studied (
P
= 0.001). Responsiveness was also increased for 72 mice tested in later pregnancy. Counts of PFC of 72% of mice 11 to 18 days pregnant were above the median when ranked with those of their controls (
P
< 0.001). In 18 of 21 litters studied, pregnant animals responded better than littermate controls (
P
= 0.001). Responses of mice at term (19 to 21 days) were depressed when compared with those of controls, but nursing animals 6 to 9 postpartum responded like virgin animals. Previous studies suggested that low concentrations of estradiol stimulate the mitosis of PFC. To determine if progesterone also increases numbers of PFC, concentrations in a range physiological for pregnancy were added to cultures of spleen cells from male mice injected with
E. coli
3 days before. After 24 h of incubation, numbers of PFC in these suspensions were compared to those of the same suspensions incubated without hormone. In 19 of 25 and 48 of 68 suspensions tested at concentrations of 500 pg and 50 ng of progesterone per ml, the numbers of PFC were increased over those of the same suspensions without hormone (
P
< 0.01). Geometric mean number of PFC for progesterone-treated cells was 45% greater than that for the controls. Findings suggest that female sex hormones, important for the normal growth and differentiation of fetal cells, may also enhance division and/or maturation of immunocompetent cells in the mother.
Pathology in rabbits treated with leukocyte-degraded meningococci in combination with meningococcal endotoxin
Authors: DeVoe I W , Gilka F , Gilchrist J E , Yu E
Published: January 2020

The effects of a preparative dose of the leukocyte egesta containing degraded meningococci and a provocative dose of the meningococcal lipopolysaccharide on development of pathological lesions associated with disseminated intravascular coagulation were studied in tissues of 32 rabbits. These effects were compared with effects of a single dose of meningococcal lipopolysaccharide as well as leukocyte egesta containing degraded Staphylococcus epidermidis. Rabbits injected subcutaneously with egesta containing degraded meningococci followed after 12 h with meningococcal endotoxin (intravenously) exhibited heterophilic leukocytosis and disseminated intravascular coagulation mainly in the pulmonary capillaries and venules; focal necroses occurred in myocardium, lungs, and liver, whereas, cortical renal necrosis developed in lethal cases. Similar lesions, however, but less severe and with less frequency, developed even after a single dose of meningococcal endotoxin or after endotoxin that followed a dose of supernatant fluid from normal leukocytes. Our findings suggest that meningococcal material from polymorphonuclear degradation plays a role in the pathology characteristic of meningococcal septicemia.
Cellular Immune Responses in Guinea Pigs Immunized with Cell Walls of
Histoplasma capsulatum
Prepared by Several Different Procedures
Authors: Domer Judith E. , Ichinose H.
Published: January 2020

Since guinea pigs immunized with water-washed cell walls of
Histoplasma capsulatum
developed cellular immune responses detectable with cytoplasmic substances, attempts were made to determine whether cytoplasmic contamination of the walls was responsible for the induction of the immune response. Cell walls were treated by several procedures designed to remove possible contamination, namely, extraction with lipid solvents, incubation with proteolytic enzymes, and washing with sodium dodecyl sulfate, and each of the treated preparations was compared with water-washed walls for its ability to induce cellular responses demonstrable with cytoplasmic substances. For comparison, wall glycoprotein was also used as a test antigen. Immune responses were assessed by gross and histological examinations of skin test sites and by assays for the production of migration inhibition factor. A portion of the material inducing the response detectable with cytoplasmic substances was apparently removed or altered by each of the purifying procedures. The cellular immune responses to wall glycoprotein were also altered, however, indicating that more than the mere removal of cytoplasmic substances had occurred. On the basis of the data collected from each of the cellular assays involving wall glycoprotein as the test antigen, the hypothesis is proposed that sodium dodecyl sulfate altered or removed protein from the wall and thus augmented its ability to induce a more intense immediate-type hypersensitivity, whereas incubation with Pronase altered the walls in such a way as to shift the balance toward a more intense delayed-type hypersensitivity. The latter effect was probably due to the removal of carbohydrate from the wall by glucanase or to mannosidase contaminating the Pronase preparation.
Catabolism of glucose and fatty acids by virulent Treponema pallidum
Authors: Schiller N L , Cox C D
Published: January 2020

We describe a procedure which permits essentially full recovery of physiologically active Treponema pallidum from rabbit testicular extracts and greatly reduces contaminating tissue material. Such preparations were employed for investigations of the ability of T. pallidum to catabolize glucose and fatty acids. Radiorespirometric studies revealed that glucose and pyruvate, but not oleate or palmitate, could be degraded to CO2. The use of differentially labeled glucose, in conjunction with enzymatic analyses, indicated that glucose was catabolized by a combination of the Embden-Meyerhoff-Parnas and hexose monophosphate pathways. Pyruvate was degraded to CO2 from only the carboxyl position, suggesting the absence of a functioning Krebs cycle; this was substantiated by additional enzyme analyses and radiorespirometric experiments. Oleate and palmitate were incorporated but not catabolized by beta-oxidation. Glucose, although catabolized, was not incorporated. The potential significance of these findings is discussed.
Pathogenesis of neonatal Escherichia coli meningitis: induction of bacteremia and meningitis in infant rats fed E. coli K1
Authors: Glode M P , Sutton A , Moxon E R , Robbins J B
Published: January 2020

Escherichia coli K1 strains, isolated from human newborns with meningitis, were fed to pathogen-free Sprague-Dawley infant rats by an oral gastric tube. Feeding of 10(3) to 10(11) organisms colonized the intestine of approximately 70% of the animals. At 5 days postfeeding of 3- to 5-day-old rats, bacteremia was detected in 60%, and meningitis occurred in 15% of bacteremic animals. Colonization and bacteremia were age-related. Rats 15 days old had only 19 colonization and 10% bacteremia, and those 30 days old were almost completely resistant to colonization and bacteremia. The intranasal route was less effective in inducing colonization and bacteremia. Intralitter transmission from E. coli K1-fed rats occurred, with 52% of water-fed controls becoming colonized and 15% become bacteremic. Colonization of mothers from their fed infants occurred, but none of five tested developed bacteremia. Other E. coli capsular polysaccharide types were studied. A K92 strain isolated from a newborn with meningitis induced a 77% colonization rate, and 8% of these developed bacteremia without detectable meningitis. An E. coli K100 strain showed a 32% colonization rate, and 2% developed bacteremia. The age relation, relatively high virulence of K1 compared with other capsular types, spontaneous appearance of colonization, bacteremia, and meningitis, and intralitter transmission of colonization and disease in newborn rats closely parellel E. coli epidemiology in human neonates.
Composition of the lipopolysaccharide of Neisseria gonorrhoeae
Authors: Wiseman G M , Caird J D
Published: January 2020

Analysis of glycose and fatty acid content of lipopolysaccharide extracted from 38 strains of Neisseria gonorrhoeae indicated that glycoses common to colonial types 1 to 5 were glucose, mannose, and galactose, N-acetylneuraminic acid, 2-keto-3-deoxyoctulosonic acid (KDO), glucosamine, and galactosamine were also invariably present. Virulent colonial types 1 and 2 contained no rhamnose, in contrast to avirulent types 3 to 5 and several strains of the nonpathogenic species N. sicca and N. lactamica. Fucose, characteristic of these nonpathogenic species, was not present in the gonococci. Variation in the concentration of individual glycoses in different strains was also noted. Mannose-KDO, galactose-KDO, and glucose-KDO ratios of virulent gonococci exceeded those of avirulent organisms, except that the correlation for glucose was not quite so striking. This relationship was not found in N. sicca and N. lactamica strains. Fatty acid analyses of lipid A from gonococci showed that 10-, 12-, 14-, 16-, and 18-carbon acids, as well as 3-hydroxytetradecanoic acid, were present, but differences in concentration between colonial types, although evident in some cases, appeared less significant than glycose content.
Thymidine kinaseless revertants of Ltk- cells transformed by herpes simplex virus type 1 are resistant to retransformation by homologous virus
Authors: Chadha K C , Munyon W H , Hughes R G
Published: January 2020

Mouse L cells lacking thymidine kinase (Ltk-) that had been transformed to the thymidine kinase-positive (tk+) phenotype by herpes simplex virus type 1 (HSV-1) were cultured in medium containing tritiated thymidine. Six clonal lines of cells surviving this treatment were found to have the following properties: (i) the cells were tk- and had spontaneous back-reversion frequencies to the tk+ phenotype of 10(-5) or less, (ii) the cells contained HSV antigens, although in lesser amounts than in the parental transformed cells, and (iii) the cells were retransformable to the tk+ phenotype by HSV-1 at frequencies of about 1 to 13% of the frequency of the primary transformation of LtK- cells. HSV-1 plaqued as efficiently on monolayers of these cells and replicated in them to the same extent as it did in Ltk- cells. These results indicate that HSV-1-transformed L cells surviving selection with tritiated thymidine are unlike the parental Ltk- cells in that they are damaged in such a way that the cells are resistant to retransformation by homologous virus, although they remain fully permissive for virus replication.
Alveolar macrophage-lymphocyte rosette formation: failure of levamisole to alter activity
Authors: Ziprin R L , Fowler S R , Witzel D A
Published: January 2020

Levamisole, an anthelminthic agent with immunostimulatory properties, does not alter in vitro macrophage-lymphocyte rosette formation between sheep alveolar macrophages and autochthonous lymphocytes.
Adjuvanticity (immunity-inducing property) of cord factor in mice and rats
Authors: Saito R , Nagao S , Takamoto M , Sugiyama K , Tanaka A
Published: January 2020

Cord factor, a glycolipid in mycobacteria, was found to make a tolerogenic protein antigen immunogenic when injected 0 to 2 days before tolerogen injection in mice and rats. Cord factor was also found to increase phagocytic function of the reticuloendothelial system of mice, and the relationship between the immunity-inducing capacity and the phagocytic function is discussed.
Effect of temperature on bacterial killing by serum and by polymorphonuclear leukocytes
Authors: Sebag J , Reed W P , Williams R C
Published: January 2020

Bacterial killing by serum alone and by polymorphonuclear )PMN) leukocytes was studied at 37 degrees C and compared with killing at 39 and 41 degrees C. The test organisms for serum killing were Staphylococcus aureus 502A (serum resistant) and Escherichia coli O14 (serum sensitive). The organisms used in PMN killing tests were Streptococcus pneumoniae type 29 and E. coli O86.S aureus was not killed by serum alone at any temperature. Changes in temperature did not affect the rate of serum killing of E. coli O14 for the first 60 min, but by 90 and 120 min there was a discrepancy with continued killing at 37 degrees C, but no further killing at 39 and 41 degrees C. PMN phagocytic killing of the pneumococcus was enhanced at 39 degrees C compared with 37 degrees C, and phagocytic killing of E. coli O86 was decreased at 41 degrees C when compared with 37 degrees C. Therefore, it appears that under certain circumstances fever may aid the host PMNs in destroying organisms, whereas under other circumstances it may interfere with such destruction.
Rough mutant of Shigella flexneri 2a that penetrates tissue culture cells but does not evoke keratoconjunctivitis in guinea pigs
Authors: Okamura N , Nakaya R
Published: January 2020

A rough mutant, designated 5503-01, has been isolated from a virulent strain of Shigella flexneri 2a 5503. Strain 5503-01 produced smooth opaque colonies, whereas its parent strain produced characteristic green-gold translucent ones. Characterization of 5503-01 by agglutination tests, rhamnose content, and sensitivity spectra to "rough-specific" phages revealed that it had lost the specific somatic antigens. When 5503-01 was used to infect HeLa or L cells, it penetrated the cells and multiplied within the cytoplasm. On the other hand, it could not evoke keratoconjunctivitis in guinea pigs. The properties of this strain were remarkably stable against serial passages and preservation for a long period. The presence of a rough mutant with the ability to penetrate tissue culture cells suggest that specific O antigen is not of significance in the early step of the invasive process of shigellosis.
Chemiluminescence spectra of human myeloperoxidase and polymorphonuclear leukocytes
Authors: Andersen B R , Brendzel A M , Lint T F
Published: January 2020

The light emission spectra of myeloperoxidase-H2O2-Cl- and phagocytizing polymorphonuclear leukocytes were estimated by a computer simulation technique by using light transmittance data from nine band-pass filters. No shift in the chemiluminescence spectrum of either system was observed during the course of the reactions, suggesting that the light-generating mechanisms remain constant after their initiation. Transmittances were virtually identical for both myeloperoxidase-H2-O2-Cl- and polymorphonuclear leukocyte reactions, suggesting that the light-generating mechanisms are identical and leading to the estimation of nearly identical spectra. Both spectra were broad, with maximum light emission near 570 nm.
Genetics of macrophage-controlled resistance to hepatitis induced by herpes simplex virus type 2 in mice
Authors: Mogensen S C
Published: January 2020

The genetics of innate resistance of mice to hepatitis induced by herpes simplex virus type 2 (HSV-2) was analyzed by crossing resistant male GR to susceptible female BALB/c mice and backcrossing females of this F1 generation to susceptible male BALB/c mice. By scoring of macroscopic liver lesions and virus isolation studies from the liver 4 days after intraperitoneal inoculation of HSV-2, it appeared that the resistance was governed by one X-linked dominant gene or closely linked gene complex, as F1 female mice were resistant and F1 male mice were susceptible and the trait segregated in a ratio close to 1:1 in the backcross mating. A cellular expression in vitro of virus resistance was found in the macrophage population of the mice as measured by differences in the restriction of HSV-2 replication in macrophage cultures prepared from individual mice. In contrast to what was seen in macrophage cultures, virus replicated equally well in embryonic fibroblast cultures from susceptible and resistant strains of mice.
Production by mixed lymphocyte cultures of a type II interferon able to protect macrophages against virus infection
Authors: Virelizier J L , Allison A C , de Maeyer E
Published: January 2020

In supernatants of mixed mouse spleen cell cultures established for 4 days, a species-specific inhibitor of virus replication with a broad antiviral spectrum was found. The inhibitor was destroyed by trypsin, was nondialyzable and acid labile, and was not neutralized by antibody to mouse L cell interferon. This indicates that in mixed lymphocyte cultures a type II interferon is made that has no immunological relationship with "fibroblast" interferon. This leukocyte product was shown to protect mouse hepatitis viruses. It is suggested that lymphocyte interferon may collaborate with macrophages in host defense against viruses, as a mediator of cellular immunity.
Commitment to deoxyribonulceic acid synthesis and the cell cycle in endotoxin-stimulated murine spleen cells
Authors: Axelrod B J , Shands J W
Published: January 2020

Events associated with endotoxin-induced mitogenesis in murine spleen cells were investigated. Commitment to deoxyribonucleic acid (DNA) synthesis, the onset of DNA synthesis, and phases of cell cycle were timed. Increased levels of DNA synthesis in murine spleen cells stimulated with endotoxin were observed 12 to 16 h after the addition of the mitogen. The total cell cycle time of stimulated B-cells was 11 to 14 h. The S-phase was 8 h. The G2-phase was 1 h, and the combined M-plus G1-phase of cycling cells was 2 to 5 h. A 1- to 4-h exposure to lipopolysaccharide elicited a significant increase in DNA synthesis. Progressively longer exposures to lipopolysaccharide, up to 24 h, produced further increases in first-cycle DNA synthesis. Polymyxin B, when added with endotoxin to cultures from the outset, inhibited first-cycle DNA synthesis. However, if addition of the antibiotic was delayed, progressive increases in first-cycle dna synthesis were observed. These data indicate a heterogeneity among B-cells in their responsiveness to endotoxin.
Effect of vitamin A on the systemic and local antibody responses to intragastrically administered bovine serum albumin
Authors: Falchuk K R , Walker W A , Perrotto J L , Isselbacher K J
Published: January 2020

The effects of vitamin A on the immune response to bovine serum albumin (BSA) were studied in adult mice. Treatment with vitamin A by the intragastric or parenteral routes markedly increased the local as well as the systemic antibody response to different concentrations of the antigen (BSA). In contrast, in animals given BSA alone, antigen concentrations above a certain dose resulted in a decreased or even absent anti-BSA response. These studies suggest that vitamin A may be an appropriate adjuvant in oral immunization.
Effect of alkali-treated lipopolysaccharide on the intracellular cations of human erythrocytes
Authors: Warren J R , Kowalski M M , Wallas C H
Published: January 2020

The adsorption to human erythrocytes of Escherichia coli lipopolysaccharide treated by mild alkaline hydrolysis (h-LPS) stimulated an increase in the intracellular Na+ concentration and a decrease in the intracellular K+ concentration of the erythrocytes. Erythrocytes treated by h-LPS remained responsive to the membrane adenosine triphosphatase inhibitors ouabain and ethacrynic acid, indicating that hLPS did not alter erythrocyte cations be depleting energy intermediates or uncoupling energy metabolism from active cation transport. The h-LPS-treated erythrocytes became non-agglutinable by the lectin concanavalin A prior to the development of changes in intracellular cations. In addition, h-LPS-treated erythrocytes demonstrated a three-fold greater cation response to ethacrynic acid than the untreated erythrocytes; this greater response was probably due to local membrane effects by h-LPS on the ethacrynic acid-sensitive adenosine triphosphatase. It is suggested that the h-LPS-induced alteration of erythrocyte cation content was secondary to an increase in ion permeability localized to the concanavalin A receptor regions of the erythrocyte membrane, possibly combined with indirect effects of membrane-bound h-LPS on ethacrynic acid-sensitive adenosine triphosphatase.
Immunochemical studies on the galactomannans isolated from mycelia and culture broths of three Hormodendrum strains
Authors: Suzuki S , Takeda N
Published: January 2020

Six major antigenic galactomannans isolated from both mycelia and culture broths of three representative strains of three species of the genus Hormodendrum, H. pedrosoi IFO 6071, H. compactum IFO 6726, and H. dermatitidis IFO 6421, were investigated for their immunochemical properties by precipitin reaction and the passive cutaneous anaphylaxis test. We found that partial acid hydrolysis of the these galactomannans with 0.005 M sulfuric acid yielded free galactose and corresponding acid-resistant core moieties. In agar-gel double-diffusion reactions, no acid-resistant core moiety of galactomannan showed a corresponding precipitin line against homologous antiserum, indicating that the acid-labile galactofuranosyl residues were solely responsible for the precipitin activity of these galactomannans. All galactomannans and their core moieties gave sharp cross-precipitin lines against anti-Saccharomyces cerevisiae serum, specific for the alpha1 leads to 3- or alpha1 leads to 2-linked D-mannopyranosyl residue. Quantitative precipitin reactions of the same antigen-antibody systems showed that all acid-resistant core moieties were completely inactive in the homologous systems but were highly cross-reactive with anti-S. cerevisiae serum. Passive cutaneous anaphylaxis tests using guinea pigs sensitized with homologous antisera or with anti-S. cerevisiae serum provided results consistent with those obtained in the in vitro assay.