bluuebunny/crossref_metadata_2025 huggingface dataset:
The Home Environment Is a Reservoir for Methicillin-Resistant Coagulase-Negative Staphylococci and Mammaliicocci
Authors: Røken Mari , Iakhno Stanislav , Haaland Anita Haug , Bjelland Ane Mohn , Wasteson Yngvild
Published: March 2024

Coagulase-negative staphylococci (CoNS) and mammaliicocci are opportunistic human and animal pathogens, often resistant to multiple antimicrobials, including methicillin. Methicillin-resistant CoNS (MRCoNS) have traditionally been linked to hospitals and healthcare facilities, where they are significant contributors to nosocomial infections. However, screenings of non-hospital environments have linked MRCoNS and methicillin-resistant mammaliicocci (MRM) to other ecological niches. The aim of this study was to explore the home environment as a reservoir for MRCoNS and MRM. A total of 33 households, including households with a dog with a methicillin-resistant staphylococcal infection, households with healthy dogs or cats and households without pets, were screened for MRCoNS and MRM by sampling one human, one pet (if present) and the environment. Samples were analyzed by a selective culture-based method, and bacterial species were identified by MALDI-TOF MS and tested for antibiotic susceptibility by the agar disk diffusion method. Following whole-genome sequencing, a large diversity of SCCmec elements and sequence types was revealed, which did not indicate any clonal dissemination of specific strains. Virulome and mobilome analyses indicated a high degree of species specificity. Altogether, this study documents that the home environment is a reservoir for a variety of MRCoNS and MRM regardless of the type of household.
Association of Circulating Endothelial Nitric Oxide Synthase Levels with Phosphataemia in Patients on Haemodialysis
Authors: Niepolski Leszek , Malinowska-Loba Kamila
Published: March 2024

The amount of evidence indicates that hyperphosphataemia (HP) can induce endothelial damage and significantly impair endothelial nitric oxide synthase (eNOS) expression. There are no clinical studies that have assessed HP and its correlation with circulating eNOS concentration in patients with end-stage renal disease (ESRD). Our preliminary study aimed to evaluate the relationship between plasma inorganic phosphorus (P) levels and circulating plasma eNOS concentration in patients on haemodialysis (HD). A total of 50 patients on HD were enrolled to the study. They were divided into groups according to the tertiles of P. The examined HD group was also analysed and compared with controls as a whole group; then, the group was divided into patients with and without dyslipidaemia (D) as well as into those with and without type 2 diabetes mellitus (type 2 DM). A total of 26 age-matched healthy volunteers were included in the study as the control group. The plasma levels of eNOS in HD patients are reduced in comparison to those in healthy subjects. There was no difference in plasma eNOS concentrations between HD patients with type 2 DM and those without DM as well as between those with D and without D. In the entire group of HD patients, there were positive correlations between circulating levels of eNOS and plasma P concentrations. In HD patients with D, higher systolic and diastolic blood pressure were accompanied by decreased plasma eNOS concentrations. In conclusion, HP and high blood pressure appear to decrease the circulating eNOS levels. These findings demonstrate an additional negative impact of HP on eNOS activity.
Zebrafish as a Model for Cardiovascular and Metabolic Disease: The Future of Precision Medicine
Authors: Angom Ramcharan Singh , Nakka Naga Malleswara Rao
Published: March 2024

The zebrafish (Danio rerio) has emerged as an appreciated and versatile model organism for studying cardiovascular and metabolic diseases, offering unique advantages for both basic research and drug discovery. The genetic conservation between zebrafish and humans and their high fecundity and transparent embryos allow for efficient large-scale genetic and drug-oriented screening studies. Zebrafish possess a simplified cardiovascular system that shares similarities with mammals, making them particularly suitable for modeling various aspects of heart development, function, and disease. The transparency of zebrafish embryos enables the real-time visualization of cardiovascular dynamics, offering insights into early embryonic events and facilitating the study of heart-related anomalies. In metabolic research, zebrafish provide a cost-effective platform for modeling obesity, type 2 diabetes, hyperlipidemia, and other metabolic disorders. Their high reproductive rate allows for the generation of large cohorts for robust statistical analyses, while advanced genetic tools, such as CRISPR/Cas9, enable precise gene editing with which to model specific genetic mutations associated with human diseases. Zebrafish metabolic models have been instrumental in elucidating the molecular mechanisms underlying metabolic diseases, studying the effects of environmental factors, and identifying potential therapeutic targets. Additionally, the permeability of zebrafish embryos to small molecules facilitates drug discovery and screening, offering a rapid and economical approach to identifying compounds with therapeutic potential. In conclusion, zebrafish cardiovascular and metabolic disease models continue to contribute significantly to our perception of disease pathogenesis, providing a platform for translational research and developing novel therapeutic interventions. The versatility, scalability, and genetic manipulability of zebrafish position them as an invaluable asset in unraveling the complexities of cardiovascular and metabolic diseases. This review presents an overview of the zebrafish model’s key features and contributions to investigating cardiovascular and metabolic disorders. We discuss the benefits and drawbacks of using zebrafish models to study human disease and the critical findings revealed by the progress in this endeavor to date.
Advancing Urban Life: A Systematic Review of Emerging Technologies and Artificial Intelligence in Urban Design and Planning
Authors: He Wei , Chen Mingze
Published: March 2024

The advancement of cutting-edge technologies significantly transforms urban lifestyles and is indispensable in sustainable urban design and planning. This systematic review focuses on the critical role of innovative technologies and digitalization, particularly artificial intelligence (AI), in urban planning through geo-design, aiming to enhance urban life. It begins with exploring the importance of AI and digital tools in revolutionizing contemporary urban planning practices. Through the methodology based on the Systematic Reviews and Meta-Analyses (PRISMA) protocol, this review sifts through relevant literature over the past two decades by categorizing artificial intelligence technologies based on their functionalities. These technologies are examined for their utility in urban planning, environmental modeling, and infrastructure development, highlighting how they contribute to creating smarter and more livable cities. For instance, machine learning techniques like supervised learning excel in forecasting urban trends, whereas artificial neural networks and deep learning are superior in pattern recognition and vital for environmental modeling. This analysis, which refers to the comprehensive evaluation conducted in this Systematic Review, encompasses studies based on diverse data inputs and domains of application, revealing a trend toward leveraging AI for predictive analytics, decision-making improvements, and the automation of complex geospatial tasks in urban areas. The paper also addresses the challenges encountered, including data privacy, ethical issues, and the demand for cross-disciplinary knowledge. The concluding remarks emphasize the transformative potential of innovative technologies and digitalization in urban planning, advocating for their role in fostering better urban life. It also identifies future research avenues and development opportunities. In light of our review findings, this study concludes that AI technologies indeed hold transformative promise for the field of geo-design and urban planning. They have proven instrumental in advancing predictive analytics, refining decision-making, and streamlining complex geospatial tasks. The AI’s capacity to process expansive datasets and improve urban planning accuracy has facilitated more sustainable urban development and enhanced the resilience of urban environments.
Current Treatment Options for Renal Cell Carcinoma: Focus on Cell-Based Immunotherapy
Authors: Hwang Angela , Mehra Vedika , Chhetri Jyoti , Ali Samira , Tran Maxine , Roddie Claire
Published: March 2024

Renal cell carcinoma (RCC) affects over 400,000 patients globally each year, and 30% of patients present with metastatic disease. Current standard of care therapy for metastatic RCC involve TKIs and ICIs, including combinatorial strategies, but this offers only modest clinical benefit. Novel treatment approaches are warranted, and cell-based immunotherapies for RCC hold significant promise. These are currently being tested in the pre-clinical setting and in early phase clinical trials. Here, we review the landscape of cellular immunotherapy for RCC in the context of currently available therapies, with a particular focus on defining the current best antigenic targets, the range of cell therapy products being explored in RCC, and how advanced engineering solutions may further enhance these therapies in the RCC space.
Metabolic Dysfunction-Associated Steatohepatitis and Progression to Hepatocellular Carcinoma: A Literature Review
Authors: Ghazanfar Haider , Javed Nismat , Qasim Abeer , Zacharia George Sarin , Ghazanfar Ali , Jyala Abhilasha , Shehi Elona , Patel Harish
Published: March 2024

The prevalence of metabolic-associated fatty liver disease (MAFLD) is increasing globally due to factors such as urbanization, obesity, poor nutrition, sedentary lifestyles, healthcare accessibility, diagnostic advancements, and genetic influences. Research on MAFLD and HCC risk factors, pathogenesis, and biomarkers has been conducted through a narrative review of relevant studies, with a focus on PubMed and Web of Science databases and exclusion criteria based on article availability and language. Steatosis marks the early stage of MASH advancement, commonly associated with factors of metabolic syndrome such as obesity and type 2 diabetes. Various mechanisms, including heightened lipolysis, hepatic lipogenesis, and consumption of high-calorie diets, contribute to the accumulation of lipids in the liver. Insulin resistance is pivotal in the development of steatosis, as it leads to the release of free fatty acids from adipose tissue. Natural compounds hold promise in regulating lipid metabolism and inflammation to combat these conditions. Liver fibrosis serves as a significant predictor of MASH progression and HCC development, underscoring the need to target fibrosis in treatment approaches. Risk factors for MASH-associated HCC encompass advanced liver fibrosis, older age, male gender, metabolic syndrome, genetic predispositions, and dietary habits, emphasizing the requirement for efficient surveillance and diagnostic measures. Considering these factors, it is important for further studies to determine the biochemical impact of these risk factors in order to establish targeted therapies that can prevent the development of HCC or reduce progression of MASH, indirectly decreasing the risk of HCC.
HER2 and PD-L1 Expression in Gastric and Gastroesophageal Junction Cancer: Insights for Combinatorial Targeting Approaches
Authors: Freitas Marta Baptista , Gullo Irene , Leitão Dina , Águas Lúcia , Oliveira Carla , Polónia António , Gomes Joana , Carneiro Fátima , Reis Celso Albuquerque , Duarte Henrique Oliveira
Published: March 2024

Gastric and gastroesophageal junction adenocarcinomas (GA/GEJA) are associated with a poor prognosis, primarily due to late disease diagnosis. Human Epidermal Growth Factor Receptor 2 (HER2) overexpression and programmed death-ligand 1 (PD-L1) expression are important biomarkers for treatment selection in locally advanced unresectable and metastatic GA/GEJA, and there is increasing interest in their role in earlier stages of disease. In this study, we aimed to evaluate HER2 and PD-L1 expression in a curative-intent GA/GEJA cohort to describe their expression patterns and analyze the association between HER2 expression and clinicopathological features. HER2 expression was evaluated in surgical and endoscopic submucosal dissection tumor samples, and PD-L1 was evaluated in HER2-positive cases. The clinical cohort included 107 patients, with 8.4% testing positive for HER2 (seven of whom also exhibited a PD-L1 combined positive score of ≥1. HER2 status was not significantly associated with survival outcomes. A pathologist-guided, region-specific analysis revealed that PD-L1 expression rarely overlaps with HER2-positive tumor areas. While the therapeutic implications of these observations remain unknown, these findings suggest that combination strategies targeting HER2 and PD-L1 might be directed toward distinct tumor subclones. The herein disclosed region-specific biomarker expression patterns may have important therapeutic and prognostic impacts, warranting further evaluation.
A Move towards Developing Usable Climate Change Adaptation and Mitigation Services for the Agricultural Sector
Authors: Moeletsi Mokhele E. , Tsubo Mitsuru
Published: March 2024

Dryland farming is at the center of increasing pressure to produce more food for the growing population in an environment that is highly variable and with high expectations for the standard of their production systems. While there is mounting pressure for increased productivity, the responsibility to protect the environment and diminish the agricultural sector’s carbon footprint is receiving growing emphasis. Achieving these two goals calls for a consolidated effort to ensure that the scientific community and service providers partner with farmers to create a sustainable food production system that does not harm the environment. In this paper, we studied the nature of the services present in the market and identified ways that could be used to improve the climate services available to the agricultural sector. Important factors that could increase the usability of climate services include coproduction, context-specific information, innovation, demand-driven services, timeliness of services, highly applicable information, provision of services in the correct format, services that increase user experience, specificity of services to a locale, and services that are easily accessible.
Clinical Research into Central Nervous System Inflammatory Demyelinating Diseases Related to COVID-19 Vaccines
Authors: Cheng Mei-Yun , Ho Hsuan-Chen , Hsu Jung-Lung , Wang Yi , Chen Linyi , Lim Siew-Na , Liao Ming-Feng , Ro Long-Sun
Published: March 2024

Various vaccines have been developed in response to the SARS-CoV-2 pandemic, and the safety of vaccines has become an important issue. COVID-19 vaccine-related central nervous system inflammatory demyelinating diseases (CNS IDDs) have been reported recently. We present one case of AstraZeneca vaccine-related myelin oligodendrocyte glycoprotein (MOG) antibody-associated disease and a literature review of another 78 patients published from January 2020 to October 2022. Patients were divided into three vaccine types (viral vector, mRNA, and inactivated vaccines) for further analyses. Among 79 patients with COVID-19 vaccine-related CNS IDDs, 49 (62%) cases received viral vector vaccines, 20 (25.3%) received mRNA vaccines, and 10 (12.7%) received inactivated vaccines. Twenty-seven cases (34.2%) were confirmed with autoantibodies, including fifteen patients (19%) with anti-MOG, eleven (13.9%) with anti-aquaporin 4 (AQP4), and one (1.3%) with both antibodies. Significantly, more males developed CNS IDDs post viral vector vaccines compared to mRNA and inactivated vaccines. Patients receiving mRNA vaccines were older than those receiving other types. Furthermore, mRNA and inactivated vaccines correlated more with anti-AQP4 antibodies, while viral vector vaccines showed higher MOG positivity. This research suggests potential associations between COVID-19 vaccine-related CNS IDDs and gender, age, and autoantibodies, contingent on vaccine types. Protein sequence analysis implies similarities between the S protein and AQP4/MOG. Further studies may elucidate the mechanisms of CNS IDDs, aiding vaccine selection for specific types.
Entropy Model of Rosin Autonomous Boolean Network Digital True Random Number Generator
Authors: Zong Yi , Dong Lihua , Lu Xiaoxin
Published: March 2024

A True Random Number Generator (TRNG) is an important component in cryptographic algorithms and protocols. The Rosin Autonomous Boolean Network (ABN) digital TRNG has been widely studied due to its nice properties, such as low energy consumption, high speed, strong platform portability, and strong randomness. However, there is still a lack of suitable entropy models to deduce the requirement of design parameters to ensure true randomness. The current model to evaluate the entropy of oscillator-based TRNGs is not applicable for Rosin ABN TRNGs due to low-frequency noise. This work presents a new, suitable stochastic model to evaluate the entropy of Rosin ABN TRNGs. Theoretical analysis and simulation experiments verify the correctness and the effectiveness of the model, and, finally, the appropriate sampling parameters for Rosin ABN TRNGs are given for sufficient entropy per random bit to ensure true randomness.
Community Tree Nurseries in the UK: Promise, Potential and Pitfalls
Authors: Ambrose-Oji Bianca , Pearson Maddy , Cross Emma , Shallcross Jackie , Polschneider Sarah , Hattersley Robert , FitzGerald Olivia
Published: March 2024

Forest restoration and tree-planting projects initiated as a response to climate change and biodiversity loss are increasingly important around the globe. Small-scale and community-based tree nurseries have been promoted as a potential route to meeting some local or regional supply-side bottlenecks. A study in the UK used a mixed methods approach to assess the potential contribution of community tree nurseries (CTNs) to tree material supply. Semi-structured interviews (SSIs) were undertaken with 16 CTNs across the UK to generate a sector-wide characterisation of CTNs. A UK-wide online survey assessed the total number of CTNs, production methods and volumes, biosecurity practices and benefits of community involvement. Another 13 CTNs receiving support to establish and extend their operations took part in SSIs assessing their development. The results indicate that there are four broad types of CTN (Organisation- and project-based, Community-based, Enterprise and Network CTNs). A significant number of CTNs in Britain are new and establishing. The sustainability of CTNs relies in large part on grant support rather than income from tree sales. Production is almost exclusively native broadleaves and local provenances. There are policy implications concerning: i. the suitability of species being produced to future climatic and market conditions and ii. the tension around financial viability, grant dependence and CTN sustainability for those CTNs focused on social and environmental benefits rather than income generation. Identifying gaps in the provision of trees by commercial nurseries, e.g., uncommon or recalcitrant species, could be a feasible financial strategy. It is likely that financial support and skills development are likely to be required as social innovation in the community-supported tree nursery sector establishes and develops. Judging the success and impact of some types of CTNs in terms of production and finance measures may be unfair, as other social and environmental benefits may be as important and valuable. It is clear that more evaluative research is needed to quantify and better understand these kinds of outcomes and the added value they accrue.
The Potential of Acorn Extract Treatment on PUFAs Oxidative Stability: A Case Study on Fish Cooking Wastewater
Authors: Araújo-Rodrigues Helena , Ribeiro Tânia Bragança , Machado Manuela , Pereira Carlos D. , Pintado Manuela E.
Published: March 2024

Fish byproducts are valuable sources of Ω-3 polyunsaturated fatty acids (PUFAs). Their valorization potentially alleviates pressure on this sector. This study uses a circular economy approach to investigate the oil fraction from sardine cooking wastewater (SCW). Analysis of its fatty acid (FA) profile revealed promising PUFA levels. However, PUFAs are highly susceptible to oxidation, prompting the exploration of effective and natural strategies to replace synthetic antioxidants and mitigate their associated risks and concerns. An antioxidant extract from acorn shells was developed and evaluated for its efficacy in preventing oxidative degradation. The extract exhibited significant levels of total phenolic compounds (TPC: 49.94 and 22.99 mg TAE or GAE/g DW) and antioxidant activities (ABTS: 72.46; ORAC: 59.60; DPPH: 248.24 mg TE/g DW), with tannins comprising a significant portion of phenolics (20.61 mg TAE/g DW). LC-ESI-UHR-QqTOF-MS identified ellagic acid, epicatechin, procyanidin B2 and azelaic acid as the predominant phenolic compounds. The extract demonstrated the ability to significantly reduce the peroxide index and inhibit PUFA oxidation, including linoleic acid (LA), eicosapentaenoic (EPA), and docosahexaenoic acid (DHA). This approach holds promise for developing stable, functional ingredients rich in PUFAs. Future research will focus on refining oil extraction procedures and conducting stability tests towards the development of specific applications.
Evaluation of Thai Silkworm (Bombyx mori L.) Hydrolysate Powder for Blood Pressure Reduction in Hypertensive Rats
Authors: Anuduang Artorn , Mustapha Wan Aida Wan , Lim Seng Joe , Jomduang Somchai , Phongthai Suphat , Ounjaijean Sakaewan , Boonyapranai Kongsak
Published: March 2024

The angiotensin-I converting enzyme (ACE) plays a pivotal role in hypertension, and while ACE inhibitors are conventional in hypertension management, synthetic medications often carry undesirable side effects. This has spurred interest in alternative ACE inhibitors derived from natural sources, such as edible insects. The silkworm, recognized for its bioactive peptides with potent ACE-inhibitory properties, has emerged as a promising candidate. This study aims to evaluate the acute toxicity and assess the antihypertensive efficacy of crude mature silkworm hydrolysate powder (MSHP) obtained from mature Thai silkworms. Utilizing the commercial protease Alcalase®2.4L, MSHP was administered at various doses, including 50, 100, and 200 mg kg−1, to hypertensive rats. The investigation spans a 14-day period to observe any potential acute toxic effects. Results indicate that MSHP exhibits LD50 values equal to or exceeding 2000 mg kg−1, signifying a low level of acute toxicity. Furthermore, the effective dose for blood pressure reduction in hypertensive rats surpasses 100 mg kg−1 of rat body weight. These findings suggest that MSHP derived from Thai mature silkworms holds promise as a natural antihypertensive food source. The implications of this research extend to the development of functional foods, functional ingredients, and dietary supplements aimed at managing hypertension.
Breaking the Taboo: Understanding the Relationship between Perception, Beliefs, Willingness to Eat Insects, and Food Neophobia among Polish Adults
Authors: Szlachciuk Julita , Żakowska-Biemans Sylwia
Published: March 2024

Insects are a potential source of protein for direct human consumption or indirectly in processed foods. The research examined consumer perceptions and beliefs regarding the consumption of insects and food products containing insect proteins. The study aimed to identify beliefs about insect consumption, assess the perception of food products containing added insect proteins, and gain a deeper understanding of the role of food neophobia in accepting insects as an alternative source of protein. The data were collected in 2023 through a cross-sectional quantitative online survey of a group that was representative of consumers by age, gender, and region sample (n = 1000) who were responsible for food purchasing decisions in their households. While many respondents viewed foods enriched with insect protein as innovative and environmentally friendly, others found the idea of consuming insects repulsive and expressed concerns about potential allergic reactions. Food neophobia was a determining factor in respondents’ beliefs and perceptions regarding the consumption of insects and food products containing insect proteins. Respondents were more inclined to consume products with added insect protein rather than visibly identifiable insects. The results highlight the need for targeted marketing strategies and product development approaches to align with the varied expectations of consumers in the rapidly expanding insect-based food market.
The Socio-Cultural Factors Influencing the Level of Public Compliance with Infection Control Measures during the 1918 Influenza Pandemic in Italy: A Historical Approach
Authors: Tognotti Eugenia , Dettori Marco
Published: March 2024

During health emergencies, non-pharmaceutical interventions (NPIs) are adopted in various combinations until a vaccine can be produced and widely administered. Containment strategies, including the closure of schools, churches, and dance halls; banning of mass gatherings; mandatory mask wearing; isolation; and disinfection/hygiene measures, require reasonable compliance to be successfully implemented. But what are the most effective measures? To date, few systematic studies have been conducted on the effects of various interventions used in past epidemics/pandemics. Important contributions to our understanding of these questions can be obtained by investigating the historical data from the great influenza pandemic of 1918, an event widely considered one of the greatest natural disasters in human history. Taking on particular importance is the study of the possible role played by the behaviour of the population and the lack of public obedience to the non-pharmaceutical interventions in a Mediterranean country like Italy—one of the most affected countries in Europe—during that pandemic, with special attention paid to the weight of the socio-cultural factors which hindered the ultimate goal of containing the spread of the virus and preventing excess deaths in the country.
Status and Barriers of Physical Activity and Exercise in Community-Dwelling Stroke Patients in South Korea: A Survey-Based Study
Authors: Lee Jung-Lim , Kim Yuna , Huh Sungchul , Shin Yong-Il , Ko Sung-Hwa
Published: March 2024

This study aimed to examine the physical activity and exercise status of stroke patients in the community after discharge and the need for community-based exercises. This study included 100 community-dwelling patients with stroke in South Korea. The survey investigated the self-assessment of health status and physical activity, demand for community-based exercise after discharge, quality of life, and social participation. Overall, 96% of the respondents recognized the need to exercise, and two-thirds exercised. The third who did not exercise cited disability (29%), lack of facilities (22%), and health concerns (13%); only 21% of participants had ever used a community exercise facility, and their satisfaction with the facility was low. The main reasons for not using community exercise facilities were concerns about accidents during exercise and accessibility issues. Among real-world community stroke patients, those who exercised regularly had higher EuroQol-5D and reintegration to normal living indices than those who did not exercise (p < 0.05). Although community-dwelling stroke patients were highly aware of the need for physical activity and exercise, few engaged in adequate exercise. This lack of engagement is directly linked to identifiable personal and socio-structural barriers. Addressing these barriers will improve the quality of life and social participation of patients with stroke.
Does the Physiological Age of Stock Plant Material Affect the Uptake of Indole-3-Butyric Acid (IBA) in Leafy Cuttings of Prunus subhirtella ‘Autumnalis’?
Authors: Kunc Petra , Medic Aljaz , Veberic Robert , Osterc Gregor
Published: March 2024

The aim of this study was to investigate the uptake of exogenously added indole-3-butyric acid (IBA) as an adventitious root (AR) inducer. The concentration of indole-3-butyric acid (IBA) in leafy cuttings of Prunus subhirtella ‘Autumnalis’ from stock material of different physiological ages was analyzed at three evaluation time points (1, 4, 24 h). We examined three stock plants: a physiologically mature stock plant (approximately 60 years old); physiologically rejuvenated plants (16 years old) that were previously propagated by cuttings from semi-mature stock material; and in vitro juvenile stock material. For IBA to effectively induce AR formation, it must first be converted into the active hormone indole-3-acetic acid (IAA). The total concentration of IAA in the first 24 h after cutting was evaluated according to the physiological age of the stock material and the IAA concentration in the cuttings according to the evaluation time points was assessed. We also examined rooting success and the quality of the adventitious root system depending on the physiological age of the stock material. High performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS) was used to identify and quantify the phytohormones. The difference in rooting and quality of the developed AR was observed between semi-mature stock and mature stock material. Cuttings from semi-mature plants rooted in 95.00 ± 5.00% of cases, while cuttings from mature stock plants only rooted in 68.33 ± 4.09%. Successfully rooted cuttings from mature stock material were accompanied by callus formation, which was significantly lower in cuttings from semi-mature stock material. Our results show that the interaction between physiological age and time after severance had no effect on IBA concentration in the cuttings (p = 0.907). Duration of time elapsed since severing (evaluation time points) had a significant effect on the uptake of IBA (p = 0.002 **) and IAA (p = 0.0009 ***) in the cuttings. Physiological age had a significant effect on IAA concentration in the early stages of AR formation in the cuttings (p = 0.038 *). Our results suggest that the dynamics of other endogenous phytohormones and the physiological state of the stock plant are also important for AR formation, as the proportion of successfully rooted cuttings from semi-mature stock material was significantly higher than the proportion of successfully rooted cuttings from mature stock material, irrespective of IBA uptake in stock material of different ages.
Physiological and Biochemical Responses of Melilotus albus to Saline and Alkaline Stresses
Authors: Wang Zhao , You Jia , Xu Xiaoyue , Yang Ying , Wang Jianli , Zhang Dongmei , Mu Linlin , Zhuang Xu , Shen Zhongbao , Guo Changhong
Published: March 2024

Sweet clover (Melilotus albus) is a high-quality leguminous forage grass with salinity tolerance, drought tolerance, and cold hardiness. We selected four varieties of sweet clover with different sensitivities (061898, 061930, No. 55 white flower, and Ningxia white flower) and analyzed the effects of different concentrations of three sodium salts (Na2CO3, NaHCO3, and NaCl) on their physiology and biochemistry responses. Growth and development indexes (such as germination rate, root length, shoot length), chlorophyll content, osmotic regulators (proline, soluble sugar), malondialdehyde (MDA), superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were determined under saline–alkali stress. Seed germination and seedling growth of all four clover species were significantly inhibited under saline–alkali stress. During germination, seed germination rate, root length, and shoot length decreased with increasing saline and alkaline concentration. Under saline–alkali stress, chlorophyll content tended to increase and then decrease, cell damage and death increased, and malondialdehyde, soluble sugar, and proline content tended to increase and then decrease. Moreover, the activities of SOD, POD, and CAT all increased and then decreased. Under Na2CO3 stress, the decrease in chlorophyll content of the resistant variety 061898 was less than in the sensitive Ningxia white flower variety. As the concentrations of Na2CO3, NaHCO3, and NaCl increased, the maximum photochemical efficiency of PSII was significantly affected. The resistant 061898 is capable of maintaining higher photosynthetic efficiency. Furthermore, under treatments with the three kinds of saline–alkali solutions, cell damage and death for Ningxia white flower were greater than in 061898. For 061898, the increases in soluble sugar and proline content were greater and the increase in malondialdehyde content was less, while the antioxidant enzyme activities were higher than those in Ningxia white flower. All four sweet clover varieties had higher stress resistance with neutral than with alkaline salts. When stressed by medium to high saline–alkali concentrations, sweet clover seedlings had increased osmotic substance content, enhanced antioxidant enzyme activity, and regulated physiological metabolism. Additionally, sweet clovers regulated the expression of WRKY33, GH3, CYCD3, OXI1, MKK2, MYC2, JAZ, COI1, PYL, PP2C, TGA, and MPK3 to adapt to the saline–alkali environment and improve saline–alkali tolerance. Our analysis of the sweet clover salinity tolerance mechanism contributes to its further use and is of significant importance for addressing land salinization and promoting sustainable agricultural and pastoral practices in China.
Comparison of Crown Volume Increment in Street Trees among Six Cities in Western Countries and China
Authors: Guo Chenbing , Hu Yonghong , Qin Jun , Wu Duorun , Xu Lin , Wang Hongbing
Published: March 2024

The tree crown volume (CV), as a major indicator in the evaluation of ecological environment quality, can assess the health and carbon sequestration of urban trees. In this study, a new low-cost method, the plane calculation of angle disparity (PCAD), was employed to obtain the CV in China using satellite images from Google Earth. Meanwhile, primary data on street trees from four Western cities were acquired from online datasets. Nonparametric statistical methods showed no significant difference in CV per street tree between Beijing and Shanghai in China, ranging from 10 to 150 m3, almost one-seventh of that in the four cities (Paris and London in Europe and Los Angeles and Seattle in America). The CV of Platanus acerifolia in Paris and London exhibited values five times higher than those in Beijing and Shanghai. The annual crown volume increment (CVI) was less than 5 m3 in Beijing and Shanghai, significantly lower than in Seattle (66.55 m3). The purpose of the research was to verify the operability of the PCAD and compare the CVI in different cities all over the world, providing new ideas for urban tree management and carbon sequestration evaluation and a basis for government decision making in areas with a low CVI.
Non-Destructive Near-Infrared Moisture Detection of Dried Goji (Lycium barbarum L.) Berry
Authors: Kang Rui , Ma Te , Tsuchikawa Satoru , Inagaki Tetsuya , Chen Jun , Zhao Jian , Li Dongdong , Cui Gongpei
Published: March 2024

To detect the moisture of dried Goji (Lycium barbarum L.) berries nondestructively, a near-infrared (NIR) hyperspectral imager was used for experiments. NIR hyperspectral data were obtained and processed by standard normal variate (SNV) calculation using the MATLAB software v.R2016a. On the basis of the actual moisture of dried Goji berries, the predicted moisture was obtained based on the partial least squares (PLS) algorithm and a prediction model for the moisture of dried goji berries was established. It was found that the moisture of dried Goji berries was responsive to the NIR hyperspectral imager. The established prediction model could accurately predict the moisture of dried goji berries, and its R2-value was 0.9981. The results provide a theoretical basis for the design of non-destructive moisture-detecting equipment for dried Goji berries.
The Brain Metabolome Is Modified by Obesity in a Sex-Dependent Manner
Authors: Norman Jennifer E. , Milenkovic Dragan , Nuthikattu Saivageethi , Villablanca Amparo C.
Published: March 2024

Obesity is linked to cognitive decline and metabolic dysregulation in the brain, yet the role of sex is relatively unexplored. We sought to explore the effects of obesity and sex on the brain metabolome. In male and female ob/ob and wild-type mice, we assessed whole brain untargeted metabolomics by liquid chromatography–mass spectrometry, behavior by open field test, and cognitive function by Y-maze and Morris water maze. The metabolic profiles of ob/ob and wild-type mice differed in both sexes. There were more obesity-altered brain metabolites in males than females. Thirty-nine metabolites were unique to males, 15 were unique to females, and five were common to both sexes. Two of the common metabolites were involved in nicotinamide adenine dinucleotide homeostasis. A key feature of the metabolites identified in males was an increase in free fatty acids. In females, a unique feature was the presence of the neuro-modulatory metabolites 2-linoleoyl glycerol and taurine. The behavioral effects of obesity were only seen in females. These results demonstrate that most impacts of obesity on the brain metabolomic profile are sex-specific. Our work has implications for understanding the role of obesity in brain metabolism and the differential contribution of obesity to cognitive decline in males and females.
Genome-Wide Identification of LOX Gene Family and Its Expression Analysis under Abiotic Stress in Potato (Solanum tuberosum L.)
Authors: Zhu Jinyong , Chen Limin , Li Zhitao , Wang Weilu , Qi Zheying , Li Yuanming , Liu Yuhui , Liu Zhen
Published: March 2024

The lipoxygenases (LOXs) are non-heme iron-containing dioxygenases that play an important role in plant growth and defense responses. There is scarce knowledge regarding the LOX gene family members and their involvement in biotic and abiotic stresses in potato. In this study, a total of 17 gene family members (StLOXs) in potato were identified and clustered into three subfamilies: 9-LOX type I, 13-LOX type I, and 13-LOX type II, with eleven, one, and five members in each subfamily based on phylogenetic analysis. By exploiting the RNA-seq data in the Potato Genome Sequencing Consortium (PGSC) database, the tissue-specific expressed and stress-responsive StLOX genes in double-monoploid (DM) potato were obtained. Furthermore, six candidate StLOX genes that might participate in drought and salt response were determined via qPCR analysis in tetraploid potato cultivars under NaCl and PEG treatment. Finally, the involvement in salt stress response of two StLOX genes, which were significantly up-regulated in both DM and tetraploid potato under NaCl and PEG treatment, was confirmed via heterologous expression in yeast under salt treatment. Our comprehensive analysis of the StLOX family provides a theoretical basis for the potential biological functions of StLOXs in the adaptation mechanisms of potato to stress conditions.
Coupling Osmotic Efficacy with Biocompatibility in Peritoneal Dialysis: A Stiff Challenge
Authors: Bonomini Mario , Masola Valentina , Monaco Maria Pia , Sirolli Vittorio , Di Liberato Lorenzo , Prosdocimi Tommaso , Arduini Arduino
Published: March 2024

Peritoneal dialysis (PD) is a home-based efficacious modality for the replacement of renal function in end-stage kidney failure patients, but it is still under-prescribed. A major limitation is the durability of the dialytic technique. Continuous exposure of the peritoneum to bioincompatible conventional glucose-based solutions is thought to be the main cause of the long-term morpho-functional peritoneal changes that eventually result in ultrafiltration failure. Poor PD solution biocompatibility is primarily related to the high glucose content, which is not only detrimental to the peritoneal membrane but has many potential metabolic side effects. To improve the clinical outcome and prolong the survival of the treatment, PD-related bioincompatibility urgently needs to be overcome. However, combining dialytic and osmotic efficacy with a satisfactory biocompatible profile is proving to be quite difficult. New approaches targeting the composition of the PD solution include the replacement of glucose with other osmotic agents, and the addition of cytoprotective or osmo-metabolic compounds. Other strategies include the infusion of mesenchymal cells or the administration of orally active agents. In the present article, we review the current evidence on efforts to improve the biocompatible and functional performance of PD, focusing on studies performed in vivo (animal models of PD, human subjects on PD).
Preventing Catastrophic Cyber–Physical Attacks on the Global Maritime Transportation System: A Case Study of Hybrid Maritime Security in the Straits of Malacca and Singapore
Authors: Fenton Adam James
Published: March 2024

This paper examines hybrid threats to maritime transportation systems and their governance responses; focusing on the congested Straits of Malacca and Singapore (SOMS) as an illustrative case study. The methodology combines secondary sources with primary data from 42 expert interviews, a 28 respondent survey, and two maritime security roundtables. Key findings were that ships’ critical systems are increasingly interconnected, yet aging IT infrastructure and minimal cybersecurity awareness among crews heighten risks. Meanwhile, regional terrorist groups have previously targeted shipping and shown considerable skill in exploiting online tools, aligning with broader calls for jihadist violence. Furthermore, opportunistic piracy persists in the SOMS with the potential to disrupt shipping. Experts confirmed that maritime cybersecurity lags behind other critical infrastructure sectors and needs updated governance. Initial International Maritime Organization (IMO) guidelines lack specificity but revisions and updated IMO guidance are in process, while Port state implementation of maritime cybersecurity standards varies. Crucially, information sharing remains inadequate, even as recorded attacks increase. Findings underscore that although major hybrid incidents have not occurred, simulations and threat actors’ capabilities demonstrate potential for catastrophic collisions or cascading disruption in congested waterways. Mitigating factors like redundancy and crew training are deficient currently. Some alignment between SOMS states on maritime security cooperation exists, but not on cyber threats specifically. Key recommendations include an anonymous cyber attack reporting system, reinforced training and shipboard systems, and consolidated regional frameworks. Until these priorities are addressed, the analysis concludes that hybrid vulnerabilities in this vital global chokepoint remain a serious concern.
The Function of Different Subunits of the Molecular Chaperone CCT in the Microsporidium Nosema bombycis: NbCCTζ Interacts with NbCCTα
Authors: Xu Sheng , Chen Ying , Qi Jingru , Wang Runpeng , Wei Erjun , Wang Qiang , Zhang Yiling , Tang Xudong , Shen Zhongyuan
Published: March 2024

Chaperonin containing tailless complex polypeptide 1 (CCT) is a molecular chaperone protein that consists of eight completely different subunits and assists in the folding of newly synthesized peptides. The zeta subunit of CCT is a regulatory factor for the folding and assembly of cytoskeletal proteins as individuals or complexes. In this study, the zeta subunit of Nosema bombycis (NbCCTζ) is identified for the first time. The complete ORF of the NbCCTζ gene is 1533 bp in length and encodes a 510 amino acid polypeptide. IFA results indicate that NbCCTζ is colocalized with actin and β-tubulin in the cytoplasm during the proliferative phase and that NbCCTζ is completely colocalized with NbCCTα in the cytoplasm of N. bombycis throughout the entire life cycle. Furthermore, the yeast two-hybrid assay revealed that the NbCCTζ interacts with NbCCTα. The transcriptional level of NbCCTζ is significantly downregulated by knocking down the NbCCTα gene, while the transcriptional level of NbCCTα is downregulated after knocking down the NbCCTζ gene. These results suggest that NbCCTζ may play a vital role in the proliferation of N. bombycis by coordinating with NbCCTα.
The Impact of Pronouns of Address in Job Ads from Different Industries and Companies
Authors: den Hartog Maria , Bras Sanne , Schoenmakers Gert-Jan
Published: March 2024

Some languages make a distinction between formal and informal pronouns of address. When organizations communicate in such a language, they have to choose between the formal and informal form. The goal of this paper is to explore the role of the V-T distinction in organizational communication, specifically in generic job advertisements, through two empirical studies and to obtain a preliminary evidence-based framework for V and T in organizational communication. In a corpus study, we explore which form organizations from different industry types tend to use. We find that the choice of pronoun of address is associated with industry type in Netherlandic Dutch, German, French, and Spanish, but not in Belgian Dutch. In an experimental follow-up study, we explore the effect of pronouns of address on Dutch addressees in light of the perceived personality of the companies using these forms. We find an interaction between the pronoun of address used and the level of competence a company is associated with. Based on these studies and the existing literature, we propose a framework for V and T in organizational communication. In this framework, local linguaculture and industry culture play a role in the organizational choice between V and T. Furthermore, the way in which pronouns of address affect the addressee is determined by an interplay of company characteristics and addressee characteristics.