bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Mycorrhizal Inoculation and Irrigation on Biological Properties of Sweet Pepper Rhizosphere in Organic Field Cultivation
Authors: Jamiołkowska Agnieszka , Skwaryło-Bednarz Barbara , Patkowska Elżbieta , Buczkowska Halina , Gałązka Anna , Grządziel Jarosław , Kopacki Marek
Published: November 2020

The aim of the study was to evaluate the influence of mycorrhizal fungi (MF) and irrigation on biological properties of sweet pepper rhizosphere in organic field cultivation. For this purpose, MF were applied to plants in the form of commercial mycorrhizal inoculum (Rhizophagus aggregatus, R. intraradices, Claroideoglomus etunicatum, Endogone mosseae, Funneliformis caledonium, and Gigaspora margarita) and irrigation according to the combinations: mycorrhized plants (PM), mycorrhized and irrigated plants (PMI), and irrigated plants (PI). Plants without MF and irrigation served as the absolute control (P). The study used classic and molecular techniques, assessing catalase activity, biodiversity of soil microorganisms (soil DNA analysis), and the Community-Level Physiological Profiles (CLPP) analysis using Biolog EcoPlates. The highest catalase activity was recorded in the control and mycorrhized soil sample. The highest total number of bacteria was noted in the rhizosphere of control plants (P) and irrigated plants, while the lowest number in the rhizosphere of mycorrhized and irrigated plants. Plant irrigation contributed to the increase in the total number of fungi in the rhizosphere. The rhizospheric soil of PM and PMI were characterized by the highest utilization of amines, amides, and amino acids, whereas the lowest level of utilization was detected in the P and PI rhizospheres. The highest biodiversity and metabolic activity were observed in the rhizospheres from the PMI and PM samples, whereas lower catabolic activity were recorded in the P and PI rhizospheres. The mycorrhization of crops improved the biological properties of the rhizosphere, especially under conditions of drought stress.
Origanum vulgare Essential Oil vs. a Commercial Mixture of Essential Oils: In Vitro Effectiveness on Salmonella spp. from Poultry and Swine Intensive Livestock
Authors: Di Vito Maura , Cacaci Margherita , Barbanti Lorenzo , Martini Cecilia , Sanguinetti Maurizio , Benvenuti Stefania , Tosi Giovanni , Fiorentini Laura , Scozzoli Maurizio , Bugli Francesca , Mattarelli Paola
Published: November 2020

Salmonella spp. represent a public health concern for humans and animals due to the increase of antibiotic resistances. In this scenario, the use of essential oils (EOs) could be a valid tool against Salmonella contamination of meat. This work compares the in vitro effectiveness of an Italian mixture of feed additives based on EOs (GR-OLI) with EO of Origanum vulgare L., recently admitted by European Food Safety Authority (EFSA) for animal use. Twenty-nine Salmonella serotypes isolated from poultry and pig farms were used to assess GR-OLI and O. vulgare EO antimicrobial propeties. O. vulgare EO was active on the disaggregation of mature biofilm, while GR-OLI was capable of inhibiting biofilm formation and disaggregating preformed biofilm. Furthermore, GR-OLI inhibited bacterial adhesion to Caco-2 cells in a dose-dependent manner. Both products showed inhibition of bacterial growth at all time points tested. Finally, the synergistic action of GR-OLI with commonly used antibiotics against resistant strains was investigated. In conclusion, the mixture could be used both to reduce the meat contamination of Salmonella spp. before slaughter, and in synergy with low doses of ciprofloxacin against resistant strains. Although EOs as feed additives are already used in animal husbandry, no scientific study has ever highlighted their real antimicrobial potential.
A One Health Approach to Strengthening Antimicrobial Stewardship in Wakiso District, Uganda
Authors: Musoke David , Kitutu Freddy Eric , Mugisha Lawrence , Amir Saba , Brandish Claire , Ikhile Deborah , Kajumbula Henry , Kizito Ismail Musoke , Lubega Grace Biyinzika , Niyongabo Filimin , Ng Bee Yean , O’Driscoll Jean , Russell-Hobbs Kate , Winter Jody , Gibson Linda
Published: November 2020

Antimicrobial stewardship (AMS), as one of the global strategies to promote responsible use of antimicrobials to prevent antimicrobial resistance (AMR), remains poor in many low-and middle-income countries (LMICs). We implemented a project aimed at strengthening AMS in Wakiso district, Uganda using a One Health approach. A total of 86 health practitioners (HPs), including animal health workers, and 227 community health workers (CHWs) participated in training workshops, and over 300 pupils from primary schools were sensitized on AMR, AMS, and infection prevention and control (IPC). We further established two multidisciplinary online communities of practice (CoPs) for health professionals and students, with a current membership of 321 and 162, respectively. In addition, a Medicine and Therapeutics Committee (MTC) was set up at Entebbe Regional Referral Hospital. The project evaluation, conducted three months after training, revealed that the majority of the HPs (92.2%) and CHWs (90.3%) reported enhanced practices, including improved hand washing (57.3% and 81.0%, respectively). In addition, 51.5% of the HPs reported a reduction in the quantity of unnecessary antibiotics given per patient. This project demonstrates that AMS interventions using a One Health approach can promote understanding of the prudent use of antimicrobials and improve practices at health facilities and in communities.
Chemical Composition and Antimicrobial Activity of Selected Essential Oils against Staphylococcus spp. Isolated from Human Semen
Authors: Kačániová Miroslava , Terentjeva Margarita , Štefániková Jana , Žiarovská Jana , Savitskaya Tatsiana , Grinshpan Dmitrij , Kowalczewski Przemysław Łukasz , Vukovic Nenad , Tvrdá Eva
Published: November 2020

Staphylococcus spp. is not only a commensal bacteria but also a major human pathogen that causes a wide range of clinical infections. Recent evidence suggests that Staphylococcus has the ability to colonize the reproductive system and to affect its structure and functions. The objective of this study was to determine the chemical properties and antibacterial effects of select essential oils (EOs): Amyris balsamifera L., Boswellia carterii Birdw., Canarium luzonicum (Blume) A. Gray, Cinnamomum camphora (L.) J. Presl., Cinnamomum camphora var. linaloolifera Y. Fuita, Citrus x aurantium L., Gaultheria procumbens L., Litsea cubeba (Lour.) Pers., Melaleuca ericifolia Smith., Melaleuca leucadendra L., Pogostemon cablin (Blanco) Benth., Citrus limon (L.) Osbeck, Santalum album L., and Vetiveria zizanoides (L.) Roberty against 50 Staphylococcus spp. cultures isolated from human semen, specifically Staphylococcus aureus, S. capiti, S. epidermidis, S. haemoliticus, and S. hominis. The disc diffusion and broth microdilution methods were used to assess the antimicrobial potential and to determine the minimum inhibitory concentration (MIC) of the selected EOs. The best anti-Staphylococcus activities were found with both methods for the essential oils of C. luzonicum (Blume) A. Gray, A. balsamifera, C. camphora, and P. cabli.
Taurine Enhances Iron-Related Proteins and Reduces Lipid Peroxidation in Differentiated C2C12 Myotubes
Authors: Seidel Ulrike , Lüersen Kai , Huebbe Patricia , Rimbach Gerald
Published: November 2020

Taurine is a nonproteinogenic amino sulfonic acid in mammals. Interestingly, skeletal muscle is unable to synthesize taurine endogenously, and the processing of muscular taurine changes throughout ageing and under specific pathophysiological conditions, such as muscular dystrophy. Ageing and disease are also associated with altered iron metabolism, especially when there is an excess of labile iron. The present study addresses the question of whether taurine connects cytoprotective effects and redox homeostasis in a previously unknown iron-dependent manner. Using cultured differentiated C2C12 myotubes, the impact of taurine on markers of lipid peroxidation, redox-sensitive enzymes and iron-related proteins was studied. Significant increases in the heme protein myoglobin and the iron storage protein ferritin were observed in response to taurine treatment. Taurine supplementation reduced lipid peroxidation and BODIPY oxidation by ~60 and 25%, respectively. Furthermore, the mRNA levels of redox-sensitive heme oxygenase (Hmox1), catalase (Cat) and glutamate-cysteine ligase (Gclc) and the total cellular glutathione content were lower in taurine-supplemented cells than they were in the control cells. We suggest that taurine may inhibit the initiation and propagation of lipid peroxidation by lowering basal levels of cellular stress, perhaps through reduction of the cellular labile iron pool.
Protective Effects of a Discontinuous Treatment with Alpha-Lipoic Acid in Obesity-Related Heart Failure with Preserved Ejection Fraction, in Rats
Authors: Pop Cristina , Ștefan Maria-Georgia , Muntean Dana-Maria , Stoicescu Laurențiu , Gal Adrian Florin , Kiss Béla , Morgovan Claudiu , Loghin Felicia , Rochette Luc , Lauzier Benjamin , Mogoșan Cristina , Ghibu Steliana
Published: November 2020

Obesity induces hemodynamic and humoral changes that are associated with functional and structural cardiac remodeling, which ultimately result in the development of heart failure (HF) with preserved ejection fraction (HFpEF). In recent years, pharmacological studies in patients with HFpEF were mostly unsatisfactory. In these conditions, alternative new therapeutic approaches are necessary. The aim of our study was (1) to assess the effects of obesity on heart function in an experimental model and (2) to evaluate the efficacy of an alpha-lipoic acid (ALA) antioxidant treatment. Sprague-Dawley rats (7 weeks old) were either included in the control group (n = 6) or subjected to abdominal aortic banding (AAB) and divided into three subgroups, depending on their diet: standard (AAB + SD, n = 8), hypecaloric (AAB + HD, n = 8) and hypecaloric with discontinuous ALA treatment (AAB + HD + ALA, n = 9). Body weight (BW), glycemia, echocardiography parameters and plasma hydroperoxides were monitored throughout the study. After 36 weeks, plasma adiposity (leptin and adiponectin) and inflammation (IL-6 and TNF-alpha) markers, together with B-type natriuretic peptide and oxidative stress markers (end-products of lipid peroxidation and endogenous antioxidant systems) were assessed. Moreover, cardiac fiber diameters were measured. In our experiment, diet-induced obesity generated cardiometabolic disturbances, and in association with pressure-overload induced by AAB, it precipitated the onset of heart failure, cardiac hypertrophy and diastolic dysfunction, while producing a pro-oxidant and pro-inflammatory plasmatic status. In relationship with its antioxidant effects, the chronic ALA-discontinuous treatment prevented BW gain and decreased metabolic and cardiac perturbations, confirming its protective effects on the cardiovascular system.
Towards the Natural Language Processing as Spelling Correction for Offline Handwritten Text Recognition Systems
Authors: Neto Arthur Flor de Sousa , Bezerra Byron Leite Dantas , Toselli Alejandro Héctor
Published: November 2020

The increasing portability of physical manuscripts to the digital environment makes it common for systems to offer automatic mechanisms for offline Handwritten Text Recognition (HTR). However, several scenarios and writing variations bring challenges in recognition accuracy, and, to minimize this problem, optical models can be used with language models to assist in decoding text. Thus, with the aim of improving results, dictionaries of characters and words are generated from the dataset and linguistic restrictions are created in the recognition process. In this way, this work proposes the use of spelling correction techniques for text post-processing to achieve better results and eliminate the linguistic dependence between the optical model and the decoding stage. In addition, an encoder–decoder neural network architecture in conjunction with a training methodology are developed and presented to achieve the goal of spelling correction. To demonstrate the effectiveness of this new approach, we conducted an experiment on five datasets of text lines, widely known in the field of HTR, three state-of-the-art Optical Models for text recognition and eight spelling correction techniques, among traditional statistics and current approaches of neural networks in the field of Natural Language Processing (NLP). Finally, our proposed spelling correction model is analyzed statistically through HTR system metrics, reaching an average sentence correction of 54% higher than the state-of-the-art method of decoding in the tested datasets.
Diabetic Macular Edema Characterization and Visualization Using Optical Coherence Tomography Images
Authors: Vidal Plácido L. , de Moura Joaquim , Díaz Macarena , Novo Jorge , Ortega Marcos
Published: November 2020

Diabetic Retinopathy and Diabetic Macular Edema (DME) represent one of the main causes of blindness in developed countries. They are characterized by fluid deposits in the retinal layers, causing a progressive vision loss over the time. The clinical literature defines three DME types according to the texture and disposition of the fluid accumulations: Cystoid Macular Edema (CME), Diffuse Retinal Thickening (DRT) and Serous Retinal Detachment (SRD). Detecting each one is essential as, depending on their presence, the expert will decide on the adequate treatment of the pathology. In this work, we propose a robust detection and visualization methodology based on the analysis of independent image regions. We study a complete and heterogeneous library of 375 texture and intensity features in a dataset of 356 labeled images from two of the most used capture devices in the clinical domain: a CIRRUSTM HD-OCT 500 Carl Zeiss Meditec and 179 OCT images from a modular HRA + OCT SPECTRALIS® from Heidelberg Engineering, Inc. We extracted 33,810 samples for each type of DME for the feature analysis and incremental training of four different classifier paradigms. This way, we achieved an 84.04% average accuracy for CME, 78.44% average accuracy for DRT and 95.40% average accuracy for SRD. These models are used to generate an intuitive visualization of the fluid regions. We use an image sampling and voting strategy, resulting in a system capable of detecting and characterizing the three types of DME presenting them in an intuitive and repeatable way.
Evaluation of the Built-Up Area Dynamics in the First Ring of Cluj-Napoca Metropolitan Area, Romania by Semi-Automatic GIS Analysis of Landsat Satellite Images
Authors: Dolean Bogdan-Eugen , Bilașco Ștefan , Petrea Dănuț , Moldovan Ciprian , Vescan Iuliu , Roșca Sanda , Fodorean Ioan
Published: November 2020

The accentuated dynamics of the real estate markets of the last 20 years, determined that a large part of the territories in the immediate vicinity of the big urban centers, to change their category of land use, in an accelerated rhythm. Most of the time, the land use changes according to the market requirements, the predominantly agricultural lands being occupied by constructions with residential or industrial functions. Identifying these changes is a difficult task due to the heterogeneity of spatial databases that come from different real estate development projects, so determining and implementing new methods to track land changes are currently highly required. This paper presents a methodologically innovative index-based approach for the rapid mapping of built-up areas, using Landsat-5, Landsat-7, and Landsat-8 satellite imagery. The approach described in this study differs from other conventional methods by the way the analysis was performed and also by the thematic indices used in the processes of built-up area delineation. The method, structured in a complex model, based on Remote Sensing and GIS techniques, can be divided into three distinct phases. The first stage is related to the pre-processing of the remote sensing data. The second stage involves the calculation of the normalized difference vegetation index (NDVI), the modified normalized difference water index (MNDWI), and the bare soil index (BI) correlated with the extraction of all areas not covered by vegetation; respectively, the elimination from the result of all areas covered by water, bare land, or uncultivated arable land. The result of this stage is represented by a distinct thematic layer that contains only built-up areas and other associated territories. The last step of the model is represented by the validation of the results, which was performed based on statistical methods and also by direct comparison with field reality, obtaining a validation coefficient which is generally above 85% for any of the methods used. The validation process shows us that by applying this method, the fast mapping of the built-up areas is significantly enhanced and the model is suitable to be implemented on a larger scale in any practical and theoretical application that aims at the rapid mapping of the built-up areas and their evolutionary modeling.
A Comparison of Rice Straw and Whole-Crop Barley (Hordeum vulgare L.) Silage Supplements on Performance and Carcass Characteristics of Hanwoo (Bos taurus coreanae) Steers
Authors: Yang Seung Hak , Kim Won Ho , Kang Suk-Nam , Choi Ki Choon , Kim Dahye
Published: November 2020

This study aimed to analyze the effects of whole-crop barley silage (WCBS) and a high-concentrate diet combination to replace a rice straw/concentrate on growth performance, and meat quality of Hanwoo steers (HS). Twenty-four animals were allocated to two groups with a rice straw/concentrate control treatment (CON) and a WCBS/concentrate treatment group. The concentrate was offered to all animals based on live weight after a feeding trial of grass-based diets. Feed intake of WCBS was lower than the CON (p < 0.01) and intake of concentrate was lower in the WCBS group than the CON in all experimental phases (p < 0.01). WCBS increased backfat thickness (BFT) and slaughter weight (p > 0.05). The marbling score was slightly higher in the WCBS group (p > 0.05). WCBS-fed beef had higher crude fat, lower crude protein, and moisture contents. WCBS feeding increased the levels of alpha-linolenic acid (p < 0.01) and decreased arachidonic acid in meat (p < 0.02). Hanwoo steers fed WCBS/concentrate improved feed intake, carcass traits, meat price, palatability, and FA content compared to those fed rice straw/concentrate, suggesting that WCBS is a potential source for improving the growth performance and meat quality in Hanwoo steers with significant economic efficiency.
A Machine Learning-Assisted Numerical Predictor for Compressive Strength of Geopolymer Concrete Based on Experimental Data and Sensitivity Analysis
Authors: Huynh An Thao , Nguyen Quang Dang , Xuan Qui Lieu , Magee Bryan , Chung TaeChoong , Tran Kiet Tuan , Nguyen Khoa Tan
Published: November 2020

Geopolymer concrete offers a favourable alternative to conventional Portland concrete due to its reduced embodied carbon dioxide (CO2) content. Engineering properties of geopolymer concrete, such as compressive strength, are commonly characterised based on experimental practices requiring large volumes of raw materials, time for sample preparation, and costly equipment. To help address this inefficiency, this study proposes machine learning-assisted numerical methods to predict compressive strength of fly ash-based geopolymer (FAGP) concrete. Methods assessed included artificial neural network (ANN), deep neural network (DNN), and deep residual network (ResNet), based on experimentally collected data. Performance of the proposed approaches were evaluated using various statistical measures including R-squared (R2), root mean square error (RMSE), and mean absolute percentage error (MAPE). Sensitivity analysis was carried out to identify effects of the following six input variables on the compressive strength of FAGP concrete: sodium hydroxide/sodium silicate ratio, fly ash/aggregate ratio, alkali activator/fly ash ratio, concentration of sodium hydroxide, curing time, and temperature. Fly ash/aggregate ratio was found to significantly affect compressive strength of FAGP concrete. Results obtained indicate that the proposed approaches offer reliable methods for FAGP design and optimisation. Of note was ResNet, which demonstrated the highest R2 and lowest RMSE and MAPE values.
Synthesis, Molecular Docking, Druglikeness Analysis, and ADMET Prediction of the Chlorinated Ethanoanthracene Derivatives as Possible Antidepressant Agents
Authors: Sultan Mujeeb A. , Galil Mansour S. A. , Al-Qubati Mohyeddine , Omar Mufeed M. , Barakat Assem
Published: November 2020

Ethanoanthracene cycloadducts (5–7) anti, (5–7) syn, and (5–7) dec have been synthesized from the Diels–Alder (DA) reaction of diene 1,8-dichloroanthracene 2, with the dienophiles; acrylonitrile 3, 1-cynavinyl acetate 4, and phenyl vinyl sulfone 5, individually. The steric effect of dienophile substituents were more favorable toward the anti-isomer formation as deduced from 1H-NMR spectrum. The cheminformatics prediction for (5–7) anti and (5–7) syn was investigated. The in silico anticipated anti-depression activity of the (5–7) anti and (5–7) syn compounds were investigated and compared to maprotiline 9 as reference anti-depressant drug. The study showed that steric interactions play a crucial role in the binding affinity of these compounds to the representative models; 4xnx, 2QJU, and 3GWU. The pharmacokinetic and drug-like properties of (5–7) anti and (5–7) syn exhibited that these compounds could be represented as potential candidates for further development into antidepressant-like agents.
Secure Learning Management System Based on User Behavior
Authors: Zamfiroiu Alin , Constantinescu Diana , Zurini Mădălina , Toma Cristian
Published: November 2020

The COVID-19 outbreak is an international problem and has affected people and students all over the world. When lockdowns were imposed internationally, learning management systems began to be used more than in the previous period. These systems have been used also for traditional forms of learning and not only for online learning. This pandemic has highlighted the need for online learning systems in the educational environment, but it is very important for these systems to be secure and to verify the authenticity of the students when they access a course or evaluation questions. In this period, everything is moving towards the digital world, with students that are connected from a distance to online systems. All activities in the educational environment will soon be performed digitally on learning management systems, which includes also the evaluation process of the students. In this paper, we propose a secure learning management system that uses the student’s behavior to identify if they are an authentic student or not. This system can support the teacher’s activities in the learning process and verify the authenticity of the students logged on to the system. This paper is aimed at learning management system developers, who can use the proposed algorithms in their developed platforms, and also at teachers, who should understand the importance of the identification of students on these platforms.
Collagenases MMP-1, MMP-13, and Tissue Inhibitors TIMP-1, TIMP-2: Their Role in Healthy and Complicated Pregnancy and Potential as Preeclampsia Biomarkers—A Brief Review
Authors: Nikolov Asparuh , Popovski Nikola , Hristova Irena
Published: November 2020

Extracellular matrix (ECM) turnover is characterized by a unique balance between matrix metalloproteinases’ degradation activity and their natural inhibition by collagen specific tissue inhibitors. Human uterine ECM is a complex structure, majorly consisting of proteins as fibrillar collagen types I and III, fibronectin, and laminin. Collagenases are enzymes from the matrix metalloproteinases’ family, which are predominantly involved in fibrillar collagen types I and III degradation. They are mainly represented by matrix metalloproteinase-1, -13 (MMP-1, -13), naturally inhibited by tissue inhibitors (TIMP-1, -2). The collagen structure of the uterus has been shown to be impaired in women with preeclampsia. This is a result of MMPs/TIMPs dysregulation interplay. This review article summarizes the actual available research data in the literature about the role of MMP-1, MMP-13 and TIMP-1, and TIMP-2 in collagen types I and III turnover in healthy and complicated pregnancy. Their potential use as circulating markers for diagnosis, prognosis, and monitoring of the development of preeclampsia is discussed as well.
Strategic Use of CAD-CAM Interim Restoration for the Recovery of the Vertical Dimension of Occlusion in the Posterior Partially Edentulous Jaw
Authors: Mai Hai Yen , Seo Jae-Min , Jung Jae-Kwang , Lee Du-Hyeong
Published: November 2020

Occlusal contact loss occasionally occurs following the placement of implant-supported fixed dental prostheses in the posterior region. This complication is caused by the change in the vertical dimension of occlusion after the recovery of mastication. The change is probably related to the prosthesis sinking phenomenon and previous mandibular dislocation. The use of interim prostheses could help re-establish the vertical dimension of occlusion. The definitive prostheses can then be accurately fabricated using digital techniques in the newly established vertical dimension. In this case report, we introduce a protocol incorporating a computer-aided design and computer-aided manufacturing (CAD-CAM) interim prosthesis and digital techniques to minimize the occurrence of unexpected initial occlusal changes in the prosthetic treatment of implant-supported prostheses in the posterior region.
Theoretical Study on Prestress Loss in Cross-Tensioned Concrete Pavement with BFRP Tendons
Authors: Zhang Yating , Huang Zhiyi
Published: November 2020

Cross-tensioned concrete pavement can reduce transverse joints and cracks and improve the durability of the pavement, and the decrease in slab thickness can be achieved without damaging the performance of the pavement. However, the corrosion of the steel can cause serious damage to the pavement structure, resulting in higher maintenance costs and shorter service life. Basalt fiber-reinforced polymer (BFRP) has been proven to be an effective alternative in both jointed plain concrete pavement (JPCP) and continuously reinforced concrete pavement (CRCP) due to its lightweight and corrosion-resistant properties. In this paper, a systematic theoretical method for determining the prestress loss of BFRP tendons in cross-tensioned concrete pavement was proposed, with the impact of the slab width and distribution angle of the prestressed tendon on the prestress loss being studied and compared to the results of traditional steel strands. Results showed that the proportion of the prestress loss due to anchorage deformation and prestress retraction in the prestressing stage rose with the increase in distribution angle and the decrease in slab width, while the prestress loss during the in-service stage was a constant value for both BFRP tendons and steel strands. The prestress loss of BFRP tendons was far lower than that of steel strands in both prestressing stage and in-service stage for a given slab width (3 m, 4.5 m, 9.0 m, 12.75 m) and distribution angle (20°, 25°, 30°, 35°, 40°, 45°), and the difference ranged from 6.4% to 16%, signifying the feasibility of BFRP tendons in cross-tensioned concrete pavement. Overall, the smaller the slab width, the greater the difference of the prestress loss between BFRP tendons and steel strands.
The Physical Manifestation of Side Reactions in the Electrolyte of Lithium-Ion Batteries and Its Impact on the Terminal Voltage Response
Authors: Balagopal Bharat , Chow Mo-Yuen
Published: November 2020

Batteries as a multi-disciplinary field have been analyzed from the electrical, material science and electrochemical engineering perspectives. The first principle-based four-dimensional degradation model (4DM) of the battery is used in the article to connect the interdisciplinary sciences that deal with batteries. The 4DM is utilized to identify the physical manifestation that electrolyte degradation has on the battery and the response observed in the terminal voltage. This paper relates the different kinds of side reactions in the electrolyte and the material properties affected due to these side reactions. It goes on to explain the impact the material property changes has on the electrochemical reactions in the battery. This paper discusses how these electrochemical reactions affect the voltage across the terminals of the battery. We determine the relationship the change in the terminal voltage has due to the change in the design properties of the electrolyte. We also determine the impact the changes in the electrolyte material property have on the terminal voltage. In this paper, the lithium ion concentration and the transference number of the electrolyte are analyzed and the impact of their degradation is studied.
Influence of Liquid-to-Gas Ratio on the Syngas Fermentation Efficiency: An Experimental Approach
Authors: Achinas Spyridon , Mulder Sytse Jelmer , Euverink Gerrit Jan Willem
Published: November 2020

Syngas fermentation by methanogens is a novel process to purify biogas. Methanogens are able to ferment non-desirable CO2, H2, and CO to methane. However, to use methanogens on an industrial scale, more research has to be done. There are studies that discuss the growth of methanogens on syngas in combination with acetate. In this research, growth of methanogens on syngas as sole carbon source is discussed. Effluent of an anaerobic fed-batch was selectively cultivated with syngas in 400 mL Eppendorf© bioreactors. After a period of 7 days, fifteen 120 mL flasks were filled with three different liquid-to-gas ratios (1:1, 1:3, 1:5). Results showed that different liquid-to-gas ratios change the metabolic preference of the anaerobic microbial community. Moreover, complete conversion in a four-to-eight-day period, via the carboxidotrophic pathway, was observed in all three liquid-to-gas ratios.
Optimized Planning and Evaluation of Dental Implant Fatigue Testing: A Specific Software Application
Authors: García-González Marta , Blasón-González Sergio , García-García Ismael , Lamela-Rey María Jesús , Fernández-Canteli Alfonso , Álvarez-Arenal Ángel
Published: November 2020

Mechanical complications in implant-supported fixed dental prostheses are often related to implant and prosthetic design. Although the current ISO 14801 provides a framework for the evaluation of dental implant mechanical reliability, strict adherence to it may be difficult to achieve due to the large number of test specimens which it requires as well as the fact that it does not offer any probabilistic reference for determining the endurance limit. In order to address these issues, a new software program called ProFatigue is presented as a potentially powerful tool to optimize fatigue testing of implant-supported prostheses. The present work provides a brief description of some concepts such as load, fatigue and stress-number of cycles to failure curves (S-N curves), before subsequently describing the current regulatory situation. After analyzing the two most recent versions of the ISO recommendation (from 2008 and 2016), some limitations inherent to the experimental methods which they propose are highlighted. Finally, the main advantages and instructions for the correct implementation of the ProFatigue free software are given. This software will contribute to improving the performance of fatigue testing in a more accurate and optimized way, helping researchers to gain a better understanding of the behavior of dental implants in this type of mechanical test.
Bioengineered Skin Intended as In Vitro Model for Pharmacosmetics, Skin Disease Study and Environmental Skin Impact Analysis
Authors: Sanabria-de la Torre Raquel , Fernández-González Ana , Quiñones-Vico María I. , Montero-Vilchez Trinidad , Arias-Santiago Salvador
Published: November 2020

This review aims to be an update of Bioengineered Artificial Skin Substitutes (BASS) applications. At the first moment, they were created as an attempt to replace native skin grafts transplantation. Nowadays, these in vitro models have been increasing and widening their application areas, becoming important tools for research. This study is focus on the ability to design in vitro BASS which have been demonstrated to be appropriate to develop new products in the cosmetic and pharmacology industry. Allowing to go deeper into the skin disease research, and to analyze the effects provoked by environmental stressful agents. The importance of BASS to replace animal experimentation is also highlighted. Furthermore, the BASS validation parameters approved by the OECD (Organisation for Economic Co-operation and Development) are also analyzed. This report presents an overview of the skin models applicable to skin research along with their design methods. Finally, the potential and limitations of the currently available BASS to supply the demands for disease modeling and pharmaceutical screening are discussed.
Chemopreventive Role of Apigenin against the Synergistic Carcinogenesis of Human Papillomavirus and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone
Authors: Yin Fangzheng , Zhao Lijiao , Zhang Lili , Chen Yuhe , Sun Guohui , Li Jintao , Zhang Na , Xu Yuancong , Chan Paul Kay-Sheung , Zhong Rugang
Published: November 2020

Tobacco smoke and human papillomavirus (HPV) are both crucial causes of cancer, and their cooperative carcinogenesis has drawn more attention in recent years. Apigenin (AP), a typical flavonoid abundantly found in flowers of plants, vegetables, and fruits, has been demonstrated to exert an anti-carcinogenic effect on various types of cancer. In this study, we investigated the capability of AP against malignant transformation and DNA damage of immortalized human esophageal epithelial (SHEE) cells induced by the synergism of HPV18 and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). The results indicated that the enhancement of migration, invasion, and proliferation ability of SHEE cells induced by HPV and NNK could be effectively inhibited by AP. Moreover, the levels of pyridyloxybutylated (POB)-DNA adducts induced by NNK via P450-catalyzed metabolic activation could also be significantly suppressed by AP. Further analyses on the molecular mechanism revealed that AP inhibited the synergistic carcinogenesis of NNK and HPV on SHEE cells by reducing the expression of mutp53, CDK4, Cyclin D1, and p-Rb (Ser 780), increasing caspase-3 activity, thereby arresting the cell cycle at G1 phase and promoting apoptosis of SHEE cells. We hypothesize that the decrease in NNK-induced POB-DNA adduct levels is related to the deactivation of P450 by AP, which needs to be confirmed in future studies. This study highlights that AP may be employed as a promising chemopreventive agent against cancers in smokers with an HPV infection.
Kunitz-Type Peptides from the Sea Anemone Heteractis crispa Demonstrate Potassium Channel Blocking and Anti-Inflammatory Activities
Authors: Gladkikh Irina , Peigneur Steve , Sintsova Oksana , Lopes Pinheiro-Junior Ernesto , Klimovich Anna , Menshov Alexander , Kalinovsky Anatoly , Isaeva Marina , Monastyrnaya Margarita , Kozlovskaya Emma , Tytgat Jan , Leychenko Elena
Published: November 2020

The Kunitz/BPTI peptide family includes unique representatives demonstrating various biological activities. Electrophysiological screening of peptides HCRG1 and HCRG2 from the sea anemone Heteractis crispa on six Kv1.x channel isoforms and insect Shaker IR channel expressed in Xenopus laevis oocytes revealed their potassium channels blocking activity. HCRG1 and HCRG2 appear to be the first Kunitz-type peptides from sea anemones blocking Kv1.3 with IC50 of 40.7 and 29.7 nM, respectively. In addition, peptides mainly vary in binding affinity to the Kv1.2 channels. It was established that the single substitution, Ser5Leu, in the TRPV1 channel antagonist, HCRG21, induces weak blocking activity of Kv1.1, Kv1.2, and Kv1.3. Apparently, for the affinity and selectivity of Kunitz-fold toxins to Kv1.x isoforms, the number and distribution along their molecules of charged, hydrophobic, and polar uncharged residues, as well as the nature of the channel residue at position 379 (Tyr, Val or His) are important. Testing the compounds in a model of acute local inflammation induced by the introduction of carrageenan administration into mice paws revealed that HCRG1 at doses of 0.1–1 mg/kg reduced the volume of developing edema during 24 h, similar to the effect of the nonsteroidal anti-inflammatory drug, indomethacin, at a dose of 5 mg/kg. ELISA analysis of the animals blood showed that the peptide reduced the synthesis of TNF-α, a pro-inflammatory mediator playing a leading role in the development of edema in this model.
A Rapid Immunochromatographic Method Based on a Secondary Antibody-Labelled Magnetic Nanoprobe for the Detection of Hepatitis B preS2 Surface Antigen
Authors: Cai Yangyang , Yan Jun , Zhu Li , Wang Hengliang , Lu Ying
Published: November 2020

Hepatitis B is a globally prevalent viral infectious disease caused by the hepatitis B virus (HBV). In this study, an immunochromatographic assay (ICA) for the rapid detection of hepatitis B preS2 antigen (preS2Ag) was established. The magnetic nanoparticles (MNPs) indirectly labelled with goat anti-mouse (GAM) secondary antibody were applied as a nanoprobe for free preS2 antibody (preS2Ab) capturing and signal amplification. By employing sample pre-incubation processing as well, preS2Ag-preS2Ab was sufficiently caught by the GAM-MNPs probe in 5 min. A qualitative sensitivity of 625 ng/mL was obtained by naked-eye observation within 15–20 min. A standard curve (0–5000 ng/mL) was established, with a quantitative limit of detection (LOD) of 3.6 ng/mL, based on the stability and penetrability of the magnetic signal characteristics. The proposed method for preS2Ag was rapid (~25 min, cf. ELISA ~4 h) and had a good accuracy, which was verified using an ELISA kit (relative error < 15%). Large equipment and skilled technicians were not required. The sensitivity and specificity of the developed GAM-MNPs-ICA method were 93.3% and 90% in clinical serum samples (n = 25), respectively. A good detection consistency (84%) was observed between the developed ICA method and 2 types of commercial ELISA kits, indicating that the GAM-MNPs-ICA has a potential application in large-scale screening for and point-of-care diagnosis of hepatitis B or other infectious diseases.
Carbon-Based Quantum Dots for Electrochemical Detection of Monoamine Neurotransmitters—Review
Authors: Elugoke Saheed E. , Adekunle Abolanle S. , Fayemi Omolola E. , Mamba Bhekie B. , Sherif El-Sayed M. , Ebenso Eno E.
Published: November 2020

Imbalance in the levels of monoamine neurotransmitters have manifested in severe health issues. Electrochemical sensors have been designed for their determination, with good sensitivity recorded. Carbon-based quantum dots have proven to be an important component of electrochemical sensors due to their high conductivity, low cytotoxicity and opto-electronic properties. The quest for more sensitive electrodes with cheaper materials led to the development of electrochemical sensors based on carbon-based quantum dots for the detection of neurotransmitters. The importance of monoamine neurotransmitters (NTs) and the good electrocatalytic activity of carbon and graphene quantum dots (CQDs and GQDs) make the review of the efforts made in the design of such sensors for monoamine NTs of huge necessity. The differences and the similarities between these two quantum dots are highlighted prior to a discussion of their application in electrochemical sensors over the last ten years. Compared to other monoamine NTs, dopamine (DA) was the most studied with GQDs and CQD-based electrochemical sensors.
COVID-19 and Parkinson’s Disease: Shared Inflammatory Pathways Under Oxidative Stress
Authors: Chaudhry Zahara , Klenja Donika , Janjua Najma , Cami-Kobeci Gerta , Ahmed Bushra
Published: November 2020

The current coronavirus pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in a serious global health crisis. It is a major concern for individuals living with chronic disorders such as Parkinson’s disease (PD). Increasing evidence suggests an involvement of oxidative stress and contribution of NFκB in the development of both COVID-19 and PD. Although, it is early to identify if SARS-CoV-2 led infection enhances PD complications, it is likely that oxidative stress may exacerbate PD progression in COVID-19 affected individuals and/or vice versa. In the current study, we sought to investigate whether NFκB-associated inflammatory pathways following oxidative stress in SARS-CoV-2 and PD patients are correlated. Toward this goal, we have integrated bioinformatics analysis obtained from Basic Local Alignment Search Tool of Protein Database (BLASTP) search for similarities of SARS-CoV-2 proteins against human proteome, literature review, and laboratory data obtained in a human cell model of PD. A Parkinson’s like state was created in 6-hydroxydopamine (6OHDA)-induced differentiated dopamine-containing neurons (dDCNs) obtained from an immortalized human neural progenitor cell line derived from the ventral mesencephalon region of the brain (ReNVM). The results indicated that SARS-CoV-2 infection and 6OHDA-induced toxicity triggered stimulation of caspases-2, -3 and -8 via the NFκB pathway resulting in the death of dDCNs. Furthermore, specific inhibitors for NFκB and studied caspases reduced the death of stressed dDCNs. The findings suggest that knowledge of the selective inhibition of caspases and NFκB activation may contribute to the development of potential therapeutic approaches for the treatment of COVID-19 and PD.
Microwave Plasma Formation of Nanographene and Graphitic Carbon Black
Authors: Kumal Raju R. , Gharpure Akshay , Viswanathan Vignesh , Mantri Aayush , Skoptsov George , Vander Wal Randy
Published: November 2020

Aerosol formation of novel carbons offers potential for scale and purity unmatched by condensed phase processes. A microwave driven plasma drives decarbonization of methane to form solid carbon as an aerosol. Dependent upon gas mixture, different forms of carbon are produced: 2D nanographene and a 3D graphitic carbon black analogue. TEM reveals the morphological differences and nanostructure. The ability to tune the dominant form is demonstrated by control of the CH4/Ar ratio. TGA plots reveal the change in products with feed gas composition and quality by oxidation temperature shift. Corresponding Raman analysis illustrates control of graphene content and lamellae quality by peak ratios. To test the origins of the graphitic particles and nanographene, a commercial carbon black was seeded into the microwave reactor, demonstrating a path for graphitic nanostructure evolution and confirming the molecular growth origins for the nanographene.
Therapeutic Efficacy of GC1118, a Novel Anti-EGFR Antibody, against Glioblastoma with High EGFR Amplification in Patient-Derived Xenografts
Authors: Lee Kyoungmin , Koo Harim , Kim Yejin , Kim Donggeon , Son Eunju , Yang Heekyoung , Lim Yangmi , Hur Minkyu , Lee Hye Won , Choi Seung Won , Nam Do-Hyun
Published: November 2020

We aimed to evaluate the preclinical efficacy of GC1118, a novel anti-epidermal growth factor receptor (EGFR) monoclonal antibody (mAb), against glioblastoma (GBM) tumors using patient-derived xenograft (PDX) models. A total of 15 distinct GBM PDX models were used to evaluate the therapeutic efficacy of GC1118. Genomic data derived from PDX models were analyzed to identify potential biomarkers associated with the anti-tumor efficacy of GC1118. A patient-derived cell-based high-throughput drug screening assay was performed to further validate the efficacy of GC1118. Compared to cetuximab, GC1118 exerted comparable growth inhibitory effects on the GBM tumors in the PDX models. We confirmed that GC1118 accumulated within the tumor by crossing the blood–brain barrier in in vivo specimens and observed the survival benefit in GC1118-treated intracranial models. Genomic analysis revealed high EGFR amplification as a potent biomarker for predicting the therapeutic efficacy of GC1118 in GBM tumors. In summary, GC1118 exerted a potent anti-tumor effect on GBM tumors in PDX models, and its therapeutic efficacy was especially pronounced in the tumors with high EGFR amplification. Our study supports the importance of patient stratification based on EGFR copy number variation in clinical trials for GBM. The superiority of GC1118 over other EGFR mAbs in GBM tumors should be assessed in future studies.
Patient-Derived Papillary Thyroid Cancer Organoids for Radioactive Iodine Refractory Screening
Authors: Sondorp Luc H.J. , Ogundipe Vivian M.L. , Groen Andries H. , Kelder Wendy , Kemper Annelies , Links Thera P. , Coppes Robert P. , Kruijff Schelto
Published: November 2020

Patients with well-differentiated thyroid cancer, especially papillary thyroid cancer (PTC), are treated with surgical resection of the thyroid gland. This is followed by post-operative radioactive iodine (I131), resulting in total thyroid ablation. Unfortunately, about 15-33% of PTC patients are unable to take up I131, limiting further treatment options. The aim of our study was to develop a cancer organoid model with the potential for pre-treatment diagnosis of these I131-resistant patients. PTC tissue from thirteen patients was used to establish a long-term organoid model. These organoids showed a self-renewal potential for at least five passages, suggesting the presence of cancer stem cells. We demonstrated that thyroid specific markers, a PTC marker, and transporters/receptors necessary for iodine uptake and thyroid hormone production were expressed on a gene and protein level. Additionally, we cultured organoids from I131-resistant PTC material from three patients. When comparing PTC organoids to radioactive iodine (RAI)-refractory disease (RAIRD) organoids, a substantial discordance on both a protein and gene expression level was observed, indicating a treatment prediction potential. We showed that patient-derived PTC organoids recapitulate PTC tissue and a RAIRD phenotype. Patient-specific PTC organoids may enable the early identification of I131-resistant patients, in order to reduce RAI overtreatment and its many side effects for thyroid cancer patients.
Comparative Proton and Photon Irradiation Combined with Pharmacological Inhibitors in 3D Pancreatic Cancer Cultures
Authors: Görte Josephine , Beyreuther Elke , Danen Erik H. J. , Cordes Nils
Published: November 2020

Pancreatic ductal adenocarcinoma (PDAC) is a highly therapy-resistant tumor entity of unmet needs. Over the last decades, radiotherapy has been considered as an additional treatment modality to surgery and chemotherapy. Owing to radiosensitive abdominal organs, high-precision proton beam radiotherapy has been regarded as superior to photon radiotherapy. To further elucidate the potential of combination therapies, we employed a more physiological 3D, matrix-based cell culture model to assess tumoroid formation capacity after photon and proton irradiation. Additionally, we investigated proton- and photon-irradiation-induced phosphoproteomic changes for identifying clinically exploitable targets. Here, we show that proton irradiation elicits a higher efficacy to reduce 3D PDAC tumoroid formation and a greater extent of phosphoproteome alterations compared with photon irradiation. The targeting of proteins identified in the phosphoproteome that were uniquely altered by protons or photons failed to cause radiation-type-specific radiosensitization. Targeting DNA repair proteins associated with non-homologous endjoining, however, revealed a strong radiosensitizing potential independent of the radiation type. In conclusion, our findings suggest proton irradiation to be potentially more effective in PDAC than photons without additional efficacy when combined with DNA repair inhibitors.