bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Evaluation of Microleakage in Class II Cavity in Primary Molars Restored With Glass Ionomer Cement, Zirconomer, and Cention N Using Stereomicroscope: An In Vitro Study
Authors: Patil Harshali P , Winnier Jasmin J
Published: July 2021

Background: Microleakage is defined as the passage of bacteria, fluid, molecules or ions between the cavity walls and restorative material. There are limited studies in the literature that have compared the microleakage of the newer restorative materials. Therefore, the aim of this study is to compare and evaluate microleakage in Class II cavity in primary molars restored with glass ionomer cement, zirconomer and cention N using stereomicroscope. Method: Standardized Class II cavities were prepared on the extracted primary molars All the prepared samples were divided into 3 experimental groups and were restored as follows: Group I- GIC (GC Universal Restorative); Group II- Zirconomer (SHOFU Inc., Kyoto, Japan) and Group III- Cention-N (Ivoclar Vivadent). The restored teeth were thermocycled, immersed in methylene blue dye and sectioned along the mesiodistal direction. The dye penetration at the occlusal surface and cervical surface was evaluated and compared using a stereo-microscope. Data was analysed using Krushal-Wallis test (Non-parametric ANOVA). Results: Among the three restorative materials, Cention N as compared to GIC and Zirconomer showed least microleakage at both the occlusal surface and cervical surface. Conclusion: Cention N a newer restorative material displayed lesser microleakage as compared to GIC and Zirconomer.
Comparing Periodontal Status Between Pregnant Women with Preeclampsia and Normal Women
Authors: Farhoodi Ilnaz , Mortazavi Zahra , Dargahi Roghayeh , Naghizadeh Baghi Abbas
Published: July 2021

Background: Periodontal disease is a common disorder in approximately 5%-10% of all pregnant women. The evidence suggests that periodontitis can increase the risk of preeclampsia. It seems that chronic systemic inflammation resulting from periodontal disease may be an important factor. However, some studies have ruled out any correlation between periodontal disease parameters and blood pressure. Therefore, this study was conducted to determine the correlation between periodontal disease and preeclampsia in Iranian pregnant women. Methods: This case-control study was conducted on 40 randomly selected preeclamptic patients as the case group and 40 randomly selected healthy pregnant women as the control group aged up to 35 years with gestational age of less than 34 weeks. Preeclampsia was diagnosed by a gynecologist as sustained pregnancy-induced hypertension (BP≥140/90 mm Hg within 6 hours) with proteinuria (with urine protein concentrations ≥1 mg/dl on a catheterized urine sample). All the participants underwent periodontal examinations, including the measurements of the pocket depth (PD), clinical attachment loss (CAL), bleeding on probing (BOP), and plaque index (PI) in all the teeth except the third molar and second distal molar teeth. Statistical analysis was performed using the Mann-Whitney U test and P<0.05 was considered as statistically significant. Results: The results showed that prevalence of periodontal disease was significantly higher in the preeclamptic group. The quantitative analysis of periodontal parameters between the groups indicated that mean values of the BOP, CAL, PD, and PI were significantly higher in the preeclamptic group, compared to those reported for the control group (P<0.001). Conclusions: The results of the present study showed that periodontal indices are more severe in pregnant women with preeclampsia, compared to those reported for normal pregnant subjects.
Short-term Effect of Four Root Filling Materials on the Flexural Strength of Human Root Dentin
Authors: Karkehabadi Hamed , Shahriari Shahriar , Sedaghat Faraz , Yarmohammadi Ebrahim , Abbaspourrokni Hadiseh
Published: July 2021

Background: This study aimed to assess the effects of calcium hydroxide, Biodentine, calcium-enriched mixture (CEM) cement, and mineral trioxide aggregate (MTA) on root dentin flexural strength after a 30-day exposure period. Methods: This in vitro experimental study evaluated 25 freshly extracted sound human incisors with no caries or restorations. The apical 5 mm and the coronal two-thirds of the crowns were cut such that all samples had 10 mm length. Dentin samples (n=20 in each group) were then exposed to 2 mm thickness of calcium hydroxide, Biodentine, CEM cement, MTA, or saline (control) in petri dishes for 30 days. Finally, dentin samples were subjected to a three-point bending test after the intervention, and the flexural strength data were analyzed using one-way ANOVA, Tukey’s test, and t test. Results: Thirty-day exposure to all four biomaterials decreased the flexural strength of root dentin (P<0.05). The four groups were significantly different in terms of the flexural strength of root dentin (P=0.001). The flexural strength of root dentin was significantly lower following exposure to calcium hydroxide (P=0.003), Biodentine (P=0.011), CEM cement (P=0.001), and MTA (P=0.007) compared to saline. The reduction in strength following exposure to calcium hydroxide was higher than that in Biodentine, CEM cement, and MTA groups (P<0.05) while the latter three were not significantly different in this respect (P>0.05). Conclusions: In general, all four tested biomaterials decrease the dentin strength although this reduction is more prominent by calcium hydroxide.
Evaluation of Stylohyoid Complex Patterns on Digital Panoramic Radiographs in the Local Population in Ilam, Iran
Authors: Arzani Vida , Ahmadi Pardis
Published: July 2021

Background: Stylohyoid ligament ossification is a complication that is repeatedly and accidentally observed on panoramic radiographs and may be the cause of some symptoms. Accordingly, awareness of this incidence enables the clinicians in the accurate diagnosis of head and neck pains while avoiding unneeded therapy. Methods: This was a descriptive cross-sectional study. The number of samples was 196 people who referred to an oral and maxillofacial radiology center in Ilam in 2020. Information was completed by a checklist which consisted of two parts, including a questionnaire (age, gender, history of maxillofacial trauma, history of maxillofacial surgery, and pregnancy) and a second part (including the ossification of the stylohyoid complex, its length, involved side, and the process category according to Langlais classification). Differences between the groups were compared by Student’s t test, Welch’s t test, or chi-square test (P<0.05). Results: The results revealed the influence of age on the calcification and elongation patterns of the styloid process while no significant association was found between gender and elongation and calcification patterns. The ossification of the stylohyoid complex was unilateral and bilateral in 24 (40.6%) and 35)59.3%) patients. Finally, the ossification of the stylohyoid complex was bilateral in 17 patients (48.6%) aged 40-59 years. Conclusions: The evaluation of stylohyoid complex patterns using panoramic radiography is essential, especially in patients with related symptoms. Further studies are needed to completely understand the underlying mechanism of the ossification of the styloid process and to assess different types of the styloid process and the relation between them in patients.
The Effect of Statins on Orthodontic Tooth Movements: A Meta-analysis of Animal Studies
Authors: Afshari Zohreh , Tahamtan Shabnam , Shirban Farinaz
Published: July 2021

Background: Statins are effective therapeutic agents for the treatment of cardiovascular diseases. Their favorable effects on various aspects of oral health including promising effects on bone metabolism and pleiotropic impacts such as anti-inflammatory properties made these drugs a current area of interest in the field of orthodontics. Therefore, the aim of this study was to evaluate the effects of statins on orthodontic tooth movement (OTM) in animals undergoing orthodontic treatments. Methods: Several databases were comprehensively searched for studies measuring the effects of statins on the OTM up to January 2020, including MEDLINE, ISI Web of Science, EMBASE, Scopus, and Cochrane. Animal studies evaluating the effects of statins on tooth movements in animals undergoing orthodontic treatments were selected based on the PICO model .Study selection, data extraction, risk of bias, and study quality assessment were independently performed by two reviewers. Finally, the data were analyzed using random-effects meta-analysis and the mean difference (MD) was used for comparing the outcome measures. Results: Three randomized trials were finally included in this meta-analysis. According to the Systematic Review Centre for Laboratory animal Experimentation Tool, all the included studies had at least one domain at a high risk of bias. The amount of the OTM was insignificantly lower in the statin group (MD = 0.134 mm, %95 confidence interval = -0.020-0.288, P>0.05). Conclusions: Due to the low quality and methodological inconsistencies among the included studies, conclusive confirmation regarding the effect of statins on the OTM remains debatable. Trail Registration: The protocol of this study was registered on PROSPERO (https://www.crd.york.ac.uk/ PROSPERO/) with the ID # CRD42020164155.
Dental Management of Peripheral Giant Cell Granuloma in an 8-Year-Old Boy: A Case Report
Authors: Kamali Sabeti Arghavan , Entezari Moghaddam Niloofar , Naderi Fariba
Published: July 2021

Background: Peripheral giant cell granuloma (PGCG) or the so-called giant cell epulis is the most common oral giant cell lesion. It normally appears as a soft tissue purple-red nodule. This lesion is certainly not a true neoplasm, but in nature, it may be reactive, thought to be stimulated by local irritation or trauma. Nonetheless, the exact cause is definitely not understood well. In appearance, lesions vary from smooth, uniformly outlined masses to irregularly developed, multilobed surface indentation protuberances. Margin ulcerations are occasionally observed as well. The lesions are painless, differ in size, and can cover many teeth. It may be a lesion on the gingiva or alveolar crest that is sessile or pedunculated, common with respect to the molars and incisors and occurs in reaction to the local response.
Taguchi Optimization of Catalytic Ozonation Process Using Modified Bone Char Ash for Removal of Methylene Blue from Aqueous Solution
Authors: Asgari Ghorban , Akbari Somaye
Published: July 2021

Methylene blue (MB) dye is an environmental contaminant that has been mostly used in textile industry. Taguchi orthogonal array design was employed as an optimization method to reduce the number of experiments. In this research, bone char ash modified by MgO-Fe catalyst was applied for degradation of MB in catalytic ozonation process (COP) system and operational parameters including initial MB dosages, initial pH, catalyst dose, and contact time were optimized with Taguchi method. Accordingly, the best condition for the removal of MB obtained at initial MB concentration of 20 mg/L, reaction time of 15 minutes, initial pH value of 10, and catalyst concentration of 0.1 g/L. Additionally, optimization of experimental set-up showed that the MB concentration had a notable effect on MB degradation in COP process (55.6%), and reaction time had a negligible effect (1.98%). At this condition, total organic carbon (TOC) removal was determined to be 31% but in longer time, its removal increased to 65%.
Sorption of Acid Orange 3 From Aqueous Solution Using Eggshell
Authors: Saghi Mohammad Hossien , Mohamadi Marziyeh , Rouki Sakineh , Salehzadeh Hamze , Sadeghi Shahram , Kowsari Mohammad Hasan
Published: July 2021

Dyes are used extensively in textile industries. The wastewater of these industries contains high amounts of pollutants which can be toxic, carcinogenic, and mutagenic and needs to be treated before being discharged into the environment. The aim of this study was to evaluate the application of eggshell as a sorbent for the removal of Acid Orange 3 from synthetic wastewater. This study is applied experimental research which was performed on a laboratory scale. Eggshell as a sorbent was prepared at laboratory temperature and pulverized by standard ASTM sieves in two sizes (50 and 140). The concentration of dye in the synthetic wastewater was 25 mg/L. In this study, increasing adsorbent dose from 2 to 5 g/100 mL led to an increase in the adsorption efficiency from 36.6% to 55.36% and by decreasing the size of eggshell particles from 50 to 140 mesh, the adsorption efficiency increased. The maximum adsorption took place in the first 90 minutes of the reaction. By increasing pH from 5 to 9, the process efficiency increased from 78 to 82%; however, at pH higher than 9, the adsorption efficiency decreased. Additionally, the adsorption characteristics of this pollutant on eggshell fitted Freundlich isotherm (R2 >0.989). Due to the characteristics of textile wastewater such as alkaline pH, eggshells can be used as a natural adsorbent in textile wastewater treatment.
Relationship Between Trace Element Content in the Brain of Bony Fish Species and Their Food Items in the Southwest of the Caspian Sea Due to Anthropogenic Activities
Authors: Sattari Masoud , Forouhar Vajargah Mohammad , Bibak Mehdi , Bakhshalizadeh Shima
Published: July 2021

The trace elements (TEs) in aquatic environments due to anthropogenic activities are readily available to aquatic organisms. There are 153 fish species inhabiting the Caspian Sea. However, little is known about TE (TE) concentrations in these species. In this study, we investigated the effects of TEs levels in the brain of some bony fish species inhabiting the Caspian basin. Totally, 405 fish from nine species with different feeding behaviors including Leuciscus aspius (n = 20) and Perca fluviatilis (n = 37) as piscivores, Rutilus kutum (n = 27) and Rutilus caspius (n = 71) as carnivores, Vimba persa (n = 56), Ponticola caspia (n = 25) and Tinca tinca (n = 31) as mollusivores, and Alburnus chalcoides (n = 77) and Alosa braschnikowi (n = 61) as zooplanktivores were collected from the southwest of the Caspian Sea basin from September 2017 to June 2018. The ICP-OES was used to measure TEs in the brain tissue of fish. The variability of TEs concentrations in the brain tissues of these fishes by principal component analysis (PCA) was decreased to 63.59% and 17.68% for PC1 and PC2, respectively, exhibiting that 81.27% of the total variability is associated with K, Mg, P, S, Zn, and Al. The two-dimensional diagrams showed the weight of each component in PCA. The PC1 was mostly influenced by P, Mg, K, and Zn, while the greatest value in PC2 belonged to Al. Furthermore, the entire number of elements determined in this study was found to be a suitable indicator for the distinction between fish species based on their feeding items.
Heavy Metal Contamination in Drinking Water Supplies in the Villages of Divandarreh: The Use of Geographic Information System
Authors: Ghahramani Esmaeil , Khoshniyat Ramin , Chavoshi Sonia , Ahmadi Kani Golzar Farhad
Published: July 2021

One of the important aspects of drinking water pollution is the presence of heavy metals that can create hazards for consumer’s health. This study was performed to find the concentration of heavy metals (arsenic, lead, iron, and zinc) in drinking water sources of Divandarreh in Kurdistan province and prepare its zoning map. A total of 99 wells, springs, and reservoirs were selected for sampling in 78 rural areas of Divandarreh County. Samples were collected from each source using grab sampling methods in two phases (rainy and dry seasons). To find the concentration of lead, the atomic absorption device (VARIAN 240 AA) was used, and Inductively Coupled Plasma (ICP: VARIAN 710) was used for determining the concentration of arsenic, iron, and zinc. Analysis of data was done using SPSS version 22.0 and the analysis of spatial variability and estimation of the concentration of heavy metals (preparation of zoning map) in the study area were carried out by ArcGIS software. The average concentrations of arsenic, lead, iron, and zinc in dry and rainy seasons were 1, 0.6, 62.9, 31.4, and 0.13, 2.16, 11.5, 19.8, respectively. Zinc, iron, and lead concentrations in sample No. 36 were higher than the standard level; therefore, it can be inferred that these three elements can create health problems in the future.
Modulatory Effect of Rutin on the Antitumor Activity and Genotoxicity of Cisplatin in Tumor-Bearing Mice
Authors: Prasad Rajesh , Prasad Surya Bali
Published: July 2021

Purpose: Cisplatin is a cancer chemotherapeutic drug that has been extensively used in the treatment of a variety of cancers. However, the full usage of cisplatin is limited due to its treatment associated development of multiple side effects in the host. In the present study, the modulatory effect of rutin, a type of flavonoid, on the cisplatin mediated antitumor activity and allied genotoxicity in ascites Dalton’s lymphoma (DL)-bearing mice were investigated. Methods: The antitumor activity was determined by calculating the percent increase in the life span of mice, cell viability and scanning electron microscopy (SEM) of DL cells. Further, the modulatory effect of rutin on the cisplatin-induced genotoxic effects in the same DL-bearing mice was assessed by the analysis of micronuclei, chromosomal aberration and sperm abnormality. Results: The combination treatment of mice with rutin and cisplatin showed a considerable increase in the life span of the DL-bearing mice depicting better antitumor efficacy. SEM of these DL cells showed severe membrane deformities in DL cells such as fusion of cell membrane, membrane blebbing, cell shrinkage, membrane folding and loss in microvilli from the tumor cell surface which may lead to cell death. Cisplatin alone treatment caused an increase in the frequency of chromosomal aberrations, micronuclei and sperms abnormality. However, the combination treatment of DL-bearing mice with rutin and cisplatin comparatively reduced these genotoxic effects. Conclusion: The overall findings suggest that rutin enhances the cisplatin-mediated antitumor activity and cytotoxicity against DL cells and at the same time diminishes the genotoxic effects induced by cisplatin in the DL-bearing mice.
Could Curcumin Modified Silver Nanoparticles Treat COVID-19?
Authors: Law Siukan , Lo Chuiman , Han Jie , Leung Albert Wingnang , Xu Chuanshan
Published: July 2021

None.
Covid 19-the 21st Century Pandemic: The Novel Coronavirus Outbreak and the Treatment Strategies
Authors: Joseph Sherin , Kutty Narayanan Anila
Published: July 2021

COVID -19 a global pandemic that has brought all the greater global countries to a hook. The novel coronavirus (SARS-CoV-2) outbreak was first reported in Wuhan, China which then started spreading to different countries around the world. ACE2 receptors are present in various organs but the overexpression of ACE2 at lung epithelia makes them more vulnerable to respiratory symptoms. SARS-CoV-2 binds to ACE2 receptors for entry into host cells which may serve as potential target for future therapy .Repurposing of drugs are the present strategy undertaken as the SARS-CoV-2 shows similar respiratory distress symptoms as in the case of SARS and MERS. At present the antiviral medications and vaccines are at the early stages and may take few months to years, to achieve their complete efficacy to solve the public crisis. The technological advancements have brought passive immunisation, which is an anecdotal success, but the ideal approach to future outbreaks of SARS-CoV-2 is done by vaccines that are under clinical trials. There are a large percentage of population under psychological crisis either due to the fear of infection or stress from the quarantine lives. High levels of viral loads at the initial stages cause higher chances of transmission hence immediate isolations and screening methods must be undertaken. This review mainly focuses on the treatment strategies followed with no definitive approval from authorities. This is an attempt to gather all the materialistic evidences available for now.
Dermal and Transdermal Drug Delivery through Vesicles and Particles: Preparation and Applications
Authors: Garg Unnati , Jain Karuna
Published: July 2021

Transdermal delivery over the past decade has become the field of interest for drug delivery due to its various advantages such as no first-pass metabolism, increased drug bioavailability, and easy administration. Different vesicle systems like ethosomes, liposomes, niosomes, and transferosomes along with particle systems like lipid nanoparticles, polymeric nanoparticles, carbon nanotubes, and fullerenes have been developed. These vesicles and particle systems have been developed using various easy and effective methods like cold injection method, rotary film evaporation, thin film hydration, high shear homogenization, solvent extraction method, and many more. These drug delivery systems are a very effective and feasible option for transdermal drug delivery and further developments can be made to increase their use. This article explains in detail the preparation methods and applications for these drug delivery systems.
A Recent Update: Solid Lipid Nanoparticles for Effective Drug Delivery
Authors: Pandey Sonia , Shaikh Farhinbanu , Gupta Arti , Tripathi Purnima , Yadav Jitendra Singh
Published: July 2021

Solid lipid nanoparticles are one of the developed technologies for addressing the bioavailability and targeting issues of drug delivery. In this review article, we attempted to incorporate all the essential details of SLNs like various methods of preparation, different models of SLNs, updated characterization methods, in-vivo behavior (Uptake), their applications, route of administration as well as advancements taken place in the field of delivery of biological drugs like gene vector, new adjuvant for vaccines, protein, and peptide with SLNs. Surface modified SLNs hold excellent potential for targeted and controlled drug delivery which is discussed and summarized. Based on the available data, the future success of SLNs is widened because they could be easily fabricated with various functionalities which would display enormous potential for targeting and diagnosing various diseases. This review would help the budding researchers to find out the unexplored areas of SLNs with the present discussion that reframes the potential of SLNs by gathering the various research findings of SLNs in tabular form along with the approved patent technologies of SLNs.
Design of Novel Nanoemulsion Formulations for Topical Ocular Delivery of Itraconazole: Development, Characterization and in Vitro Bioassay
Authors: Mehrandish Saba , Mirzaeei Shahla
Published: July 2021

Purpose: The objective of this study was to design and develop nanoemulsion formulations of Itraconazole (ITZ), a water-insoluble, potent antifungal drug using the spontaneous emulsification method, to improve the ocular delivery and achieve a sustained release of the drug. Methods: The oil was selected on the basis of the ITZ solubility while the surfactant and co-surfactant were selected based on the thermodynamic stability and globule size. Following the selection of components, a pseudo-ternary phase diagram was constructed for the most promising formulation (F11) using benzyl benzoate (BB) as the oil, Eumulgin CO40 as the surfactant, and propylene glycol as the co-surfactant, by the design of experiments (DoE). Results: F7 and F11 formulations were found to have an average globule size of 223.5 ± 10.7 nm and 157.5 ± 14.2 nm, besides thermodynamic stability and suitable physicochemical properties. F11 possessed an almost seven-fold higher cumulative percentage of in vitro released ITZ, in comparison to ITZ aqueous suspension after 24 hours. The release data suggested that the best fitted kinetical model for F11 and F7 was the Higuchi and Korsmeyer-Peppas model. Conclusion: The extended-release of the drug beside an acceptable amount of loaded ITZ suggested that nanoemulsion is suitable for the delivery of the ITZ.
Photosensitivity Reactions Induced by Photochemical Degradation of Drugs
Authors: Klelemen Hajnal , Hancu Gabriel , Kacsó Edina , Papp Lajos-Attila
Published: July 2021

Photochemical degradation of drugs can lead to degradation products with potential toxic or allergizing effects for the human body. A significant amount of work has been carried out over the past few decades to clarify the molecular mechanism of photosensitizing processes observed after the administration of certain drugs and exposure to light. There is a close relation between the photosensitizer effect of a drug and its chemical structure. Compounds possessing certain moieties and functional groups in their molecular structure, like aromatic chromophore systems or photo-dissociable bonds that can form free radicals, and consequently are susceptible to have light-induced adverse effects. Photoionization, photodissociation, photoaddition and photoisomerization are the main chemical processes, which can occur during the photochemical decomposition of a pharmaceutical compound. The current study is a short review describing photochemical degradation of certain pharmaceuticals, presenting specific examples from various pharmaceutical classes for the different types of decomposition mechanisms. In vivo methods and clinical tests available for the investigation of photosensitizing reactions are also discussed.
Osteogenic Differentiation Potential of Adipose-Derived Mesenchymal Stem Cells Cultured on Magnesium Oxide/Polycaprolactone Nanofibrous Scaffolds for Improving Bone Tissue Reconstruction
Authors: Niknam Zahra , Golchin Ali , Rezaei –Tavirani Mostafa , Ranjbarvan Parviz , Zali Hakimeh , Omidi Meisam , Mansouri Vahid
Published: July 2021

Purpose: Recently, bone tissue engineering as a new strategy is used to repair and replace bone defects due to limitations in allograft and autograft methods. In this regard, we prepared nanofibrous scaffolds composed of polycaprolactone and magnesium oxide nanoparticles using the electrospinning technique for possible bone tissue engineering applications. Methods: The fabricated composites were characterized via scanning electron microscopy imaging of scaffolds and seeded cells, water contact angle, DAPI staining, and MTT assay. Then osteogenic differentiation of adipose-derived mesenchymal stem cells cultured on this composite scaffold was determined by standard osteogenic marker tests, including alkaline phosphatase activity, calcium deposition, and expression of osteogenic differentiation genes in the laboratory conditions. Results: The Scanning electron microscopy analysis demonstrated that the diameter of nanofibers significantly decreased from 1029.25±209.349 µm to 537.83+0.140 nm, with the increase of MgO concentration to 2% (p<0.05). Initial adhesion and proliferation of the adipose-derived mesenchymal stem cells on magnesium oxide/polycaprolactone scaffolds were significantly enhanced with the increasing of magnesium oxide concentration (p<0.05). The 2% magnesium oxide/polycaprolactone nanofibrous scaffold showed significant increase in ALP activity (p<0.05) and osteogenic-related gene expressions (Col1a1 and OPN) (p<0.05) in compared to pure polycaprolactone and (0, 0.5 and 1%) magnesium oxide/polycaprolactone scaffolds. Conclusion: According to the results, it was demonstrated that magnesium oxide/polycaprolactone composite nanofibers have considerable osteoinductive potential, and taking together adipose-derived mesenchymal stem cells-magnesium oxide/polycaprolactone composite nanofibers can be a proper bio-implant to usage for bone regenerative medicine applications. Future in vivo studies are needed to determine this composite therapeutic potential.
microRNA‐193a‐5p Suppresses the Migratory Ability of Human KATO III Gastric Cancer Cells through Inhibition of Vimentin and MMP-9
Authors: Baghbanzadeh Amir , Baghbani Elham , Hajiasgharzadeh Khalil , Noorolyai Saeed , Khaze Vahid , Mansoori Behzad , Shirmohamadi Masoud , Baradaran Behzad , Mokhtarzadeh Ahad
Published: July 2021

Purpose: microRNA‐193a‐5p is one of the well-known tumor suppressor miRNAs in the body but in many cases, its expression became reduced in patients suffering from gastric cancer (GC). The main purpose of this study was restoring the function of this miRNA in human GC cells and investigating the effects of enhanced expression of miR‐193a‐5p on proliferation, apoptosis, and migration of GC cells upon in vitro transfection. Methods: The KATO III gastric cancer cells were treated with 100 nM of miR‐193a‐5p or negative control sequences. Following that, the MTT assay, flow cytometry assay, and wound-healing assay were applied to estimate the impacts of enhanced expression of this miRNA on the viability, apoptosis, and migration rate of the cells, respectively. Moreover, the total RNA was isolated and alterations in the mRNA expression ratio of migratory genes were measured by qRT-PCR techniques. Results: The findings designated that enhanced expression of miR‐193a‐5p suppressed the migratory ability of the cells, but had no significant effects on cell survival or apoptosis of the transfected cells. In addition, this inhibitory function of miR‐193a‐5p on the migration rate of the KATO III cell line occurs with concurrent suppression of vimentin and MMP-9 gene expression. Conclusion: It can be concluded that miR‐193a‐5p negatively influences the migratory ability of the cancerous cells and restoring its effects can be regarded as a promising target of future therapeutic interventions, especially for GC metastasis.
CEP55 Inhibitor: Extensive Computational Approach Defining a New Target of Cell Cycle Machinery Agent
Authors: Lestari Beni , Utomo Rohmad Yudi
Published: July 2021

Purpose: Centrosomal protein 55 (CEP55) is a pivotal protein for cytokinesis during cell division. This study aimed to provide a comprehensive information about the CEP55 gene, including its expression pattern in several cancer types, conduct functional domain analysis across species, and perform a computational approach for potential inhibitors of CEP55. Methods: The expression levels of CEP55 in different cancers were analyzed using the Oncomine and TCGA databases. Evolutionary analysis of the CEP55 gene in various species was performed using MEGA-X software. Molecular docking analysis was used to screen the binding affinity of several natural products on CEP55–ALIX binding interaction. Results: High CEP55 expression was observed in 16 datasets of different cancer types. The high expression of the CEP55 protein was associated with worse outcomes in cancer treatments. Phylogenetic and evolutionary analyses revealed that the amino acid residues essential for CEP55 binding and localization were mostly conserved across vertebrates. Seventeen plant-based compounds were docked against the CEP55 protein to determine their binding affinities and illustrated specific sites of interaction for predicting novel protein–drug interactions. Flavanol compounds epigallocatechin gallate and catechin possessed superior binding affinity to all other compounds owing to the substitution of gallic ester or hydroxyl groups on the C3 position. Conclusion: This study provides comprehensive information about the CEP55 gene and insights for designing potent inhibitors against CEP55 signaling.
Protective Role of Trans-chalcone against the Progression from Simple Steatosis to Non-alcoholic Steatohepatitis: Regulation of miR-122, 21, 34a, and 451
Authors: Karimi-Sales Elham , Jeddi Sajad , Ebrahimi-Kalan Abbas , Alipour Mohammad Reza
Published: July 2021

Purpose: Non-alcoholic steatohepatitis (NASH) is an inflammatory disorder and an aggressive form of fatty liver disease. Certain microRNAs, including miR-122, 21, 34a, and 451, are involved in the transition from steatosis to NASH. This study examined how trans-chalcone (the core of chalcone derivatives) affects NAFLD progression by regulating miRNAs. Methods: Male rats were divided into three groups (n = 7/group) as follows: control, rats were gavaged with 10% tween 80 (for two weeks); NASH, rats were gavaged with a high-fat liquid diet (HFD; for six weeks) and 10% tween 80 (for two weeks); NASH + Chal, rats were gavaged with the HFD (for six weeks) and trans-chalcone (for two weeks). Hepatic expression levels of miR-122, 21, 34a, and 451 were determined. Results: trans-Chalcone reversed histological abnormalities, reduced liver injury markers, and attenuated insulin resistance in HFD-fed rats. In the liver, HFD-induced NASH increased the expression level of miR-34a and decreased expression levels of miR-122, 21, and 451. However, trans-chalcone inhibited HFD-induced changes in expression levels of these miRNAs. Conclusion: trans-Chalcone could inhibit the transition from steatosis to NASH through the modulation of miR-122, 21, 34a, and 451 expression levels in the liver.
Mesenchymal Stem Cells in the Treatment of New Coronavirus Pandemic: A Novel Promising Therapeutic Approach
Authors: Razi Sara , Molavi Zahra , Mirmotalebisohi Seyed Amir , Niknam Zahra , Sameni Marzieh , Niazi Vahid , Adibi Amirjafar , Yazdani Mohsen , Ranjbar Mohammad Mehdi , Zali Hakimeh
Published: July 2021

After SARS and MERS outbreaks, COVID-19 is the third coronavirus epidemic that soon turned into a pandemic. This virus causes acute respiratory syndrome in infected people. The mortality rate of SARS-CoV-2 infection will probably rise unless efficient treatments or vaccines are developed. The global funding and medical communities have started performing more than five hundred clinical examinations on a broad spectrum of repurposed drugs to acquire effective treatments. Besides, other novel treatment approaches have also recently emerged, including cellular host-directed therapies. They counteract the unwanted responses of the host immune system that led to the severe pathogenesis of SARS-CoV-2. This brief review focuses on Mesenchymal stem cell (MSC) principles in treating the COVID-19. The US clinical trials database and the world health organization database for clinical trials have reported 82 clinical trials (altogether) exploring the effects of MSCs in COVID-19 treatment. MSCs also had better be tried for treating other pathogens worldwide. MSC treatment may have the potential to end the high mortality rate of COVID-19. Besides, it also limits the long-term inability of survivors.
Exendin-4 as a Versatile Therapeutic Agent for the Amelioration of Diabetic Changes
Authors: Rajabi Hadi , Ahmadi Mahdi , Aslani Somayeh , Saberianpour Shirin , Rahbarghazi Reza
Published: July 2021

Type 2 diabetes mellitus is a chronic metabolic abnormality leading to microvascular and macrovascular complications. Non-insulin Incretin mimic synthetic peptide exendin-4 was introduced as an anti-diabetic drug which helped diabetic patients with triggering insulin secretion; further researches have revealed an effective role of exendin-4 in treatment of type 2 diabetes mellitus related diseases. Exendin-4 is approximately similar to Glucagon-like peptide, thus it can bind to the glucagon-like peptide-1 receptor and activated different signaling pathways that are involved in various bioactivities such as apoptosis, insulin secretion and inactivation of microglial. In this review, we investigated the interesting role of exendin-4 in various kinds of type 2 diabetes mellitus related disorders through the activation of different signaling pathways.
Carrier Effect in Development of Rifampin Loaded Proliposome for Pulmonary Delivery: A Quality by Design Study
Authors: Parhizkar Elahehnaz , Sadeghinia Delaram , Hamishehkar Hamed , Yaqoubi Shadi , Nokhodchi Ali , Alipour Shohreh
Published: July 2021

Purpose: Pulmonary Tuberculosis (TB) is a worldwide life-threatening infection. The recommended anti-TB regimen contains oral administration of classical first-line drugs such as rifampin for 6-24 months which often leads to low patient compliance due to high adverse effects; therefore, lung localized pulmonary delivery of anti-TB agents may be a suitable alternative. Proliposomes free-flowing powders are well-known carriers for lung delivery since they can form liposomes by hydration. Liposomes are safe and useful carriers for lung delivery due to their phospholipid structure. Methods: Porous lactose and mannitol as proliposome carriers were prepared by spray drying technique using sucrose and citric acid as templating agents. Design expert® software was used to develop forty formulations due to the porous and non-porous carriers, which were characterized with respect to their weight yield, density, and flowability. Rifampin-loaded hydrated liposomes were produced and evaluated for size, morphology, loading capacity and encapsulation efficiency. The optimized proliposomes in vitro release and aerosolization properties were evaluated. Solid-state analysis was confirmed by Differential Scanning Calorimetry (DSC). Results: Porous lactose surface area was 80 folds higher than non-porous one, respectively. Optimized porous-based proliposome indicated the acceptable aerosolization properties, including mass median aerodynamic diameter (MMAD) of 6.21±0.36 µm and fine particle fraction (FPF) of 9.17±0.18% with a fast rifampin release (80%) within one hour. DSC results proved that there was no change in the solid-state of rifampin during the production process. Conclusion: Hence, it seems; rifampin loaded inhalable proliposomes may be a suitable system for delivering liposomal rifampin into the lungs.
Human Bone Marrow Mesenchymal Stromal Cells Attenuate Tissue Injury and Reduce Inflammation in Experimental Acute Pancreatitis
Authors: Mahmoudi Tayebeh , Jalili Ali , Abdolmohammadi Kamal , Fakhari Shohreh , Pahlavan Fatemeh , Shekari Ali , Nikkhoo Bahram , Tayebi Lobat , Rahmani Mohammad Reza
Published: July 2021

Purpose: Acute pancreatitis (AP) which is distinguished by local pancreatic necrosis, following by systemic organ failure is known as an inflammatory disease. Up to now, there are only a few treatment options accessible for patients suffering from AP. In this study, we aimed to examine the anti-inflammatory capacities of human bone marrow-derived mesenchymal stromal cells (hBM-MSC) in a detailed AP model experiment. Methods: AP was induced in C57BL/6 mice by intraperitoneal administration of cerulein (100 µg/kg/h × 7 doses) at intervals of 1 hour (h). Then, 2×105 MSCs were infused in the AP mice by tail vein 6 h after the last cerulein injection. Mice were sacrificed 12 h following the injection of hBM-MSC, and blood samples and pancreas tissues were obtained. Results: We first determined the presence of transplanted hBM-MSC in the pancreas of mice with AP, but not the control mice. Our data indicate that administration of hBM-MSCs to mice with AP lead to (i) decreased serum levels of amylase, lipase and myeloperoxidase activities, (ii) downregulation of proinflammatory cytokine, macrophage inflammatory protein 2 (MIP-2), and (iii) upregulation of the anti-inflammatory cytokine, interleukin 10 (IL-10). Moreover, hBM-MSC administration results in notably attenuated cerulein-induced histopathological alternations and edema. Conclusion: we demonstrate that hBM-MSC attenuates AP signs and indicating that hMB-MSC therapy could be a suitable approach for the treatment of inflammatory disease such as AP.
Downregulation of HMGA2 by Small Interfering RNA Affects the Survival, Migration, and Apoptosis of Prostate Cancer Cell Line
Authors: Khajouee Shima , Baghbani Elham , Mohammadi Ali , Mansoori Behzad , Shanehbandi Dariush , Hajiasgharzadeh Khalil , Baradaran Behzad
Published: July 2021

Purpose: To investigate the downregulation of High Mobility Group AT-hook 2 (HMGA2) expression by small interfering RNAs (siRNAs) in PC3 prostate cancer cell line. HMGA2 belongs to the non-histone chromatin-binding protein family that serves as a crucial regulator of gene transcription. The overexpression of this gene is positively correlated with various prostate cancer-related properties. Thus, HMGA2 is an emerging target in prostate cancer treatment. This study aimed to examine the impact of siRNAs targeting HMGA2 on the viability, migration, and apoptosis processes of the PC3 prostate cancer cell line. Methods: siRNA transfection was conducted with a liposome-mediated approach. The mRNA and protein expression levels for HMGA2 are evaluated by qRT-PCR and western blot analysis. The cytotoxic properties of HMGA2-siRNA were measured by MTT assay on PC3 cells. The migration of PC3 cells was measured by implementing a wound-healing assay. Apoptosis measurement was also quantified by TUNEL assay. Results: Transfection with siRNA significantly decreased both mRNA and protein levels of the HMGA2 gene in a dose-dependent manner after 48 hours. Also, we demonstrated that the knockdown of HMGA2 led to a reduction in cell viability, migration ability, and enhanced apoptosis of PC3 cells in vitro. Conclusion: Our findings recommend that the specific siRNA of HMGA2 may efficiently be able to decrease prostate cancer progression. Therefore, it may be a promising adjuvant treatment in prostate cancer.
Clinical Outcomes of Metastatic Colorectal Cancer Following Surgical Resection of the Primary tumor at Hiwa Cancer Hospital, Sulaimani, Iraq
Authors: Namiq Kadhim Faruq , Ali Kosar Mohammad , Gubari Mohammed Ibrahim Mohialdeen
Published: July 2021

Background: Colorectal cancer (CRC) is one of the main causes of cancer-related mortality. The surgical resection of primary CRC tumors is a critical line of treatment. The present study investigated the clinical outcomes of the surgical resection of the primary tumor in metastatic CRC patients. Materials and Methods: In this prospective and retrospective study, 81 metastatic CRC patients were recruited at Hiwa Cancer Hospital in Sulaimani, Iraq from January 2016 to December 2019. Forty-one patients underwent surgical resection of their primary tumor while the remaining 40 patients did not undergo resection. Data regarding patients’ clinical outcomes were obtained from the clinical portal system of the hospital and were analyzed using SPSS (version 23.0). Results: The patients’ mean (± SD) age was 53.5 (± 17.02) years and the male-to-female ratio was 1.3:1. Patients undergoing the surgical resection of their primary tumors had a significantly better one-year survival compared to those who did not undergo resection (P = 0.04). Based on the results, patients in the surgical resection group continued to have a better overall survival although it was not statistically significant (P = 0.1). Significantly more patients with colon cancer underwent surgical resection compared to rectal cancer (P = 0.03), and smoking habit negatively affected the chance to undergo surgical resection (P = 0.009). Conclusion: In general, the surgical resection of the primary tumor has a significant favorable impact on one-year survival, but possibly not on overall survival. The primary site of the tumor and smoking habits significantly influence the chance to undergo surgical resection whereas the grade of the tumor or the type of systemic therapy has no significant impact in this regard.
Job’s Syndrome With a Family History of Kawasaki Disease: A Case Presentation and Review of Literature
Authors: Yared Tamar , Mohsen Samer
Published: July 2021

Background: Job’s syndrome or hyper-immunoglobulin E (IgE) syndrome (HIES) is an extremely rare primary immunodeficiency disease with an approximate annual incidence of less than 1/1000000. It is characterized by recurrent cold staphylococcal infections, unusual eczematous dermatitis, severe lung infections, and extensively high concentrations of the serum antibody IgE. Case Presentation: A typical case of Job’s syndrome with a family history of Kawasaki disease is presented in this study aiming at identifying the clinical features, investigational procedures, and management strategy, as well as evaluating the role of the ear, nose, and throat specialist and highlighting the probable relation between Job’s syndrome and Kawasaki disease. Conclusions: In general, early detection with proper care can prevent the progression of Job syndrome. In addition, the initiated treatment at the first signs of infection is mandatory for preventing long-term complications. There is a probable relation between Job and Kawasaki which requires more consideration.
Patients’ Concerns at Their Admission in Hospital and Its Related Factors in Kermanshah, Iran
Authors: Siabani Soraya , Solouki Leila , Almasi Afshin , Siabani Sina , Khaledi Motahareh , Sepehri Elaheh , Almasi Maryam
Published: July 2021

Background: One of the critical factors affecting patients’ outcomes is their concerns about different issues during their admission to the hospital. Clarifying these concerns and providing appropriate approaches could improve the quality of care, result in better outcomes, and reduce treatment costs. The present study aimed to investigate patients’ concerns during hospitalization, and the likely related factors of the educational hospitals in Kermanshah, western Iran. Materials and Methods: This analytical-descriptive study included 600 adult patients selected via a multi-stage sampling method and admitted to all four educational hospitals affiliated to Kermanshah University of Medical Sciences )KUMS) in 2016. Required data were collected using a survey with 15 questions on demographic information, current disease, medical records, and a researcher-developed questionnaire on factors causing concern in the Likert scale. Results: Of 600 patients who participated in the survey, 336 (56%) were female and 486 )81%) were married. The most frequent concerns were the length of admission, failure in treatment or recovery, and hospital costs, respectively. The length of hospital stay, income, and level of education were significantly associated with the concern scores. Also, there was a significant difference between concern score distributions in groups with a definite diagnosis of illnesses (P<0.05). Conclusion: The results of this study suggested a correlation between variables such as education, income, the final diagnosis of a sickness, and the concern level of admitted patients. Our findings could help managers and hospital administrators better understand the concerns of admitted patients and find solutions to remove them.
Evaluating the Correlation of Hemostatic and Endocrine Parameters with Child-Turcotte-Pugh Scoring in Patients with Non-Alcoholic Liver Cirrhosis
Authors: Düğeroğlu Harun , Yılmaz Mustafa
Published: July 2021

Background: Liver cirrhosis is one of the most significant causes of death in many regions worldwide. This study aimed to assess the correlation of hemostatic and endocrine parameters with Child-Turcotte-Pugh (CTP) scoring in patients with non-alcoholic liver cirrhosis. Materials and Methods: This study included 59 patients monitored for non-alcoholic liver cirrhosis in a gastroenterology clinic from January 2018 to February 2019. The subjects were grouped according to the CTP scores, and their adrenocorticotropic hormone (ACTH), cortisol, dehydroepiandrosterone sulfate (DHEA-SO4), progesterone, growth hormone (GH), insulin-like growth factor-1 (IGF-1), GH/ IGF-1 ratio, fibrinogen, D-dimer, and D-dimer/fibrinogen ratio were measured. Results: According to CTP scoring, 20.3% of patients were CTP-A (n=12), 35.6% were CTP-B (n=21), and 44.1% were CTP-C (n=26). There were statistically significant differences for IGF-1, DHEA-SO4, and GH/IGF-1 ratios between CTP-A and CTP-B groups (P=0.002, P=0.043, P=0.038, respectively). Additionally, there were statistically significant differences between the CTP-B and CTP-C groups for D-dimer and GH values (P=0.024, P=0.006, respectively). There were statistically significant differences for D-dimer, GH, IGF-1, and D-dimer/fibrinogen ratio between the CTP-A and CTP-C groups (P<0.001, P=0.016, P=0.002, P=0.031, respectively). Conclusion: Severe hemostatic and endocrine complications develop in patients with liver cirrhosis due to non-alcoholic causes. Additionally, it seems that using D-dimer and D-dimer/fibrinogen ratio, along with fibrinogen level, can be beneficial in showing liver damage in these patients.