bluuebunny/crossref_metadata_2025 huggingface dataset:
Sensitive Detection of KRAS Mutations by Clustered Regularly Interspaced Short Palindromic Repeats
Authors: Zhou Huifen , Tsou Jen-Hui , Leng Qixin , Jiang Feng
Published: January 2021

Kirsten rat sarcoma viral oncogene (KRAS) is the isoform most frequently mutated in human tumors. Testing for activating KRAS mutations has important implications for diagnosis and the personalized medicine of cancers. The current techniques for detecting KRAS mutations have moderate sensitivity. The emerging clustered regularly interspaced short palindromic repeats (CRISPR) system shows great promise in the detection of nucleic acids and is revolutionizing medical diagnostics. This study aimed to develop CRISPR–Cas12a as a sensitive test to detect KRAS mutations. Serially diluted DNA samples containing KRAS mutations are subjected to CRISPR–Cas12a and polymerase chain reaction (PCR). CRISPR–Cas12a and PCR can specifically detect 0.01% and 0.1% mutant KRAS DNA in the presence of wild-type KRAS DNA, respectively. Twenty pairs of lung tumor and noncancerous lung tissues are tested by CRISPR–Cas12a, PCR, and direct sequencing. CRISPR–Cas12a could identify the G12C mutation in five of 20 tumor tissues, while both PCR and direct sequencing discovered the KRAS mutation in three of the five tumor tissues. Furthermore, the results of CRISPR–Cas12a for testing the mutation could be directly and immediately visualized by a UV light illuminator. Altogether, CRISPR–Cas12a has a higher sensitivity for the detection of KRAS mutations compared with PCR and sequencing analysis, and thus has diagnostic and therapeutic implications. Nevertheless, the technique needs to be validated for its clinical significance in a large and prospective study.
Subclavian Vessel Compression Assessed by Duplex Scanning in Patients with Neurogenic Thoracic Outlet Syndrome and No Vascular Signs
Authors: Fouasson-Chailloux Alban , Menu Pierre , Daley Pauline , Gautier Giovanni , Gadbled Guillaume , Abraham Pierre , Dauty Marc
Published: January 2021

Neurogenic thoracic outlet syndrome (NTOS) is the most frequent form of TOS. It may affect both sides, but specific complementary exams are lacking. We aimed to evaluate duplex scanning results in a group of patients with unilateral or bilateral NTOS and no clinical vascular signs, referred for rehabilitation. We performed a retrospective observational study in patients with unilateral or bilateral NTOS and no vascular symptoms. Subclavian vessels were assessed by duplex scanning. Compressions were considered in case of >50% of increased or decreased blood flow. A total of 101 patients met NTOS criteria; mean age was 40 +/− 10.2; 79.2% women. Seventy patients had a unilateral NTOS and 31 a bilateral form. Duplex scanning showed that 56.4% of the patients had vessels compression, 55.7% in the unilateral group and 58.1% in the bilateral (p = 0.81). In unilateral NTOS, 21 (30%) patients had bilateral vascular compression, 17 (24.3%) had ipsilateral compression and 1 (1.4%) had contralateral compression. In bilateral NTOS, 15 (48.4%) had bilateral compression and 3 (9.7%) compression on only one side. We found a significant difference of the rate of vascular compressions between symptomatic and non-symptomatic upper-limbs, 54.5% vs. 32.9%, respectively, (p = 0.002) and a significant association between symptomatic upper-limbs and vascular compression (OR = 2.45 [95%IC: 1.33–4.49]; p = 0.002). The sensitivity and the specificity of the duplex scanning were 54.5% and 67%, respectively. The ROC curve area was of 0.608 [95%IC: 0.527–0.690]. Despite a highly significant association between symptomatic upper-limbs and vascular compression, duplex scanning did not help make the diagnosis of NTOS.
Alteration of Postural Balance in Patients with Fibromyalgia Syndrome—A Systematic Review and Meta-Analysis
Authors: Núñez-Fuentes David , Obrero-Gaitán Esteban , Zagalaz-Anula Noelia , Ibáñez-Vera Alfonso Javier , Achalandabaso-Ochoa Alexander , López-Ruiz María del Carmen , Rodríguez-Almagro Daniel , Lomas-Vega Rafael
Published: January 2021

Balance problems are one of the most frequent symptoms in patients with Fibromyalgia Syndrome (FMS). However, the extent and nature of this balance disorder are not known. The objective of this work was to determine the best evidence for the alteration of postural balance in patients with FMS and analyze differences with healthy controls. To meet this objective, a systematic review with meta-analysis was performed. A bibliographical search was carried out in PubMed Medline, Scopus, Web of Science, CINAHL and SciELO. Observational studies that assessed postural balance in patients with FMS compared to healthy subjects in baseline conditions, were selected. In a random-effect model, the pooled effect was calculated with the Standardized Mean Difference (SMD) and its 95% confidence interval (CI). Nineteen studies reporting data of 2347 participants (95% female) were included. FMS patients showed poor balance with a large effect on static (SMD = 1.578; 95% CI = 1.164, 1.992), dynamic (SMD = 0.946; 95% CI = 0.598, 1.294), functional balance (SMD = 1.138; 95% CI = 0.689, 1.588) and on balance confidence (SMD = 1.194; 95% CI = 0.914, 1.473). Analysis of the Sensory Organization Test showed large alteration of vestibular (SMD = 1.631; 95% CI = 0.467, 2.795) and visual scores (SMD = 1.317; 95% CI = 0.153, 2.481) compared to healthy controls. Patients with FMS showed worse scores for different measures of postural balance compared to healthy controls. Concretely, FMS patients appear to have poor vestibular and visual scores with a possible somatosensory dependence.
Internet of Things (IoT) Based Indoor Air Quality Sensing and Predictive Analytic—A COVID-19 Perspective
Authors: Mumtaz Rafia , Zaidi Syed Mohammad Hassan , Shakir Muhammad Zeeshan , Shafi Uferah , Malik Muhammad Moeez , Haque Ayesha , Mumtaz Sadaf , Zaidi Syed Ali Raza
Published: January 2021

Indoor air quality typically encompasses the ambient conditions inside buildings and public facilities that may affect both the mental and respiratory health of an individual. Until the COVID-19 outbreak, indoor air quality monitoring was not a focus area for public facilities such as shopping complexes, hospitals, banks, restaurants, educational institutes, and so forth. However, the rapid spread of this virus and its consequent detrimental impacts have brought indoor air quality into the spotlight. In contrast to outdoor air, indoor air is recycled constantly causing it to trap and build up pollutants, which may facilitate the transmission of virus. There are several monitoring solutions which are available commercially, a typical system monitors the air quality using gas and particle sensors. These sensor readings are compared against well known thresholds, subsequently generating alarms when thresholds are violated. However, these systems do not predict the quality of air for future instances, which holds paramount importance for taking timely preemptive actions, especially for COVID-19 actual and potential patients as well as people suffering from acute pulmonary disorders and other health problems. In this regard, we have proposed an indoor air quality monitoring and prediction solution based on the latest Internet of Things (IoT) sensors and machine learning capabilities, providing a platform to measure numerous indoor contaminants. For this purpose, an IoT node consisting of several sensors for 8 pollutants including NH3, CO, NO2, CH4, CO2, PM 2.5 along with the ambient temperature & air humidity is developed. For proof of concept and research purposes, the IoT node is deployed inside a research lab to acquire indoor air data. The proposed system has the capability of reporting the air conditions in real-time to a web portal and mobile app through GSM/WiFi technology and generates alerts after detecting anomalies in the air quality. In order to classify the indoor air quality, several machine learning algorithms have been applied to the recorded data, where the Neural Network (NN) model outperformed all others with an accuracy of 99.1%. For predicting the concentration of each air pollutant and thereafter predicting the overall quality of an indoor environment, Long and Short Term Memory (LSTM) model is applied. This model has shown promising results for predicting the air pollutants’ concentration as well as the overall air quality with an accuracy of 99.37%, precision of 99%, recall of 98%, and F1-score of 99%. The proposed solution offers several advantages including remote monitoring, ease of scalability, real-time status of ambient conditions, and portable hardware, and so forth.
Android Malware Detection Based on Structural Features of the Function Call Graph
Authors: Yang Yang , Du Xuehui , Yang Zhi , Liu Xing
Published: January 2021

The openness of Android operating system not only brings convenience to users, but also leads to the attack threat from a large number of malicious applications (apps). Thus malware detection has become the research focus in the field of mobile security. In order to solve the problem of more coarse-grained feature selection and larger feature loss of graph structure existing in the current detection methods, we put forward a method named DGCNDroid for Android malware detection, which is based on the deep graph convolutional network. Our method starts by generating a function call graph for the decompiled Android application. Then the function call subgraph containing the sensitive application programming interface (API) is extracted. Finally, the function call subgraphs with structural features are trained as the input of the deep graph convolutional network. Thus the detection and classification of malicious apps can be realized. Through experimentation on a dataset containing 11,120 Android apps, the method proposed in this paper can achieve detection accuracy of 98.2%, which is higher than other existing detection methods.
Enhancement of Biodiesel Production from High-Acid-Value Waste Cooking Oil via a Microwave Reactor Using a Homogeneous Alkaline Catalyst
Authors: Hsiao Ming-Chien , Liao Peir-Horng , Lan Nguyen Vu , Hou Shuhn-Shyurng
Published: January 2021

In this study, low quality oils (waste cooking oils) with high acid value (4.81 mg KOH/g) were utilized as the feedstocks for a transesterification reaction enhanced by additional microwave power and the use of an NaOH catalyst. The kinetics of the transesterification reaction under different reaction times and temperatures was studied. It was found that in the microwave-assisted transesterification reaction, the optimum conditions under a microwave power of 600 W were as follows: an NaOH catalyst of 0.8 wt %, a 12:1 molar ratio of methanol to oil, a reaction time of 2 min, and a reaction temperature of 65 °C. The conversion of waste cooking oil into biodiesel reached 98.2% after this short reaction time. This result conformed to 96.5% of the standard value of Taiwan CNS 15072. In addition, with increases in the reaction temperature from 55 to 65 °C, the reaction rate constant increased from 0.635 to 2.396 min−1, and the activation energy required for the transesterification reaction was 123.14 kJ/mole.
Effect of Pebble Size Distribution and Wall Effect on Inner Packing Structure and Contact Force Distribution in Tritium Breeder Pebble Bed
Authors: Gong Baoping , Cheng Hao , Feng Yongjin , Luo Xiaofang , Wang Long , Wang Xiaoyu
Published: January 2021

In the tritium breeding blanket of nuclear fusion reactors, the heat transfer behavior and thermal-mechanical response of the tritium breeder pebble bed are affected by the inner packing structure, which is crucial for the design and optimization of a reliable pebble bed in tritium breeding blanket. Thus, the effect of pebble size distribution and fixed wall effect on packing structure and contact force in the poly-disperse pebble bed were investigated by numerical simulation. The results show that pebble size distribution has a significant influence on the inner packing structure of pebble bed. With the increase of the dispersion of pebble size, the average porosity and the average coordination number of the poly-disperse pebble bed gradually decrease. Due to the influence of the fixed wall, the porosity distribution of the pebble bed shows an obvious wall effect. For poly-disperse pebble bed, the influenced region of the wall effect gradually decreases with the increase of the dispersion of pebble size. In addition, the gravity effect and the pebble size distribution have an obvious influence on the contact force distribution inside the poly-disperse pebble bed. The majority of the contact force are weak contact force that is less than the average contact force. Only a few of pebbles have strong contact force that is greater than average contact force. This investigation can help in analyzing the pebble crushing characteristics and the thermal hydraulic analysis in the poly-disperse tritium breeder pebble bed.
Predicting Fermentation Rates in Ale, Lager and Whisky
Authors: Reid Struan J. , Josey Maria , MacIntosh Andrew J. , Maskell Dawn L. , Alex Speers R.
Published: January 2021

Recently there has been an increased interest in characterising the rates of alcoholic fermentations. Sigmoidal models have been used to predict changes such as the rate of density decline. In this study, three published sigmoidal models were assessed and fit to industrial fermentation data. The first is the four-parameter logistic model described in the ASBC Yeast-14 method. The second model is a nested form of the four-parameter logistic function, adding an extra parameter, creating the 5-parameter logistic equation., where an additional parameter was added to allow for asymmetry. The final model is a three-parameter logistic equation which describes the change in the Apparent Degree of Fermentation with time. The three models were compared by fitting them to industrial data from Australian and Canadian lagers, American and Scottish ales and Scotch Whisky fermentations. The model fits were then compared to one another with a technique developed by Akaike and a nested F-test. The Akaike information criterion compares the models and accounts for both the goodness of fit, and the number of parameters in the model. Finally, consideration was given to the establishment of prediction bands (that enclose the area that one can be 99% sure contains the true datapoints). Calculation of these bands was “challenging” but successful as the industrial fermentation data was heteroscedastic.
WNT-FRIZZLED-LRP5/6 Signaling Mediates Posterior Fate and Proliferation during Planarian Regeneration
Authors: Pascual-Carreras Eudald , Sureda-Gómez Miquel , Barrull-Mascaró Ramon , Jordà Natàlia , Gelabert Maria , Coronel-Córdoba Pablo , Saló Emili , Adell Teresa
Published: January 2021

An organizer is defined as a group of cells that secrete extracellular proteins that specify the fate of surrounding cells according to their concentration. Their function during embryogenesis is key in patterning new growing tissues. Although organizers should also participate in adult development when new structures are regenerated, their presence in adults has only been identified in a few species with striking regenerative abilities, such as planarians. Planarians provide a unique model to understand the function of adult organizers, since the presence of adult pluripotent stem cells provides them with the ability to regenerate any body part. Previous studies have shown that the differential activation of the WNT/β-catenin signal in each wound is fundamental to establish an anterior or a posterior organizer in the corresponding wound. Here, we identify the receptors that mediate the WNT/β-catenin signal in posterior-facing wounds. We found that Wnt1-Fzd1-LRP5/6 signaling is evolutionarily conserved in executing a WNT/β-catenin signal to specify cell fate and to trigger a proliferative response. Our data allow a better understanding of the mechanism through which organizers signal to a “competent” field of cells and integrate the patterning and growth required during de novo formation of organs and tissues.
Genetics of Peripartum Cardiomyopathy: Current Knowledge, Future Directions and Clinical Implications
Authors: Spracklen Timothy F. , Chakafana Graham , Schwartz Peter J. , Kotta Maria-Christina , Shaboodien Gasnat , Ntusi Ntobeko A. B. , Sliwa Karen
Published: January 2021

Peripartum cardiomyopathy (PPCM) is a condition in which heart failure and systolic dysfunction occur late in pregnancy or within months following delivery. Over the last decade, genetic advances in heritable cardiomyopathy have provided new insights into the role of genetics in PPCM. In this review, we summarise current knowledge of the genetics of PPCM and potential avenues for further research, including the role of molecular chaperone mutations in PPCM. Evidence supporting a genetic basis for PPCM has emanated from observations of familial disease, overlap with familial dilated cardiomyopathy, and sequencing studies of PPCM cohorts. Approximately 20% of PPCM patients screened for cardiomyopathy genes have an identified pathogenic mutation, with TTN truncations most commonly implicated. As a stress-associated condition, PPCM may be modulated by molecular chaperones such as heat shock proteins (Hsps). Recent studies have led to the identification of Hsp mutations in a PPCM model, suggesting that variation in these stress-response genes may contribute to PPCM pathogenesis. Although some Hsp genes have been implicated in dilated cardiomyopathy, their roles in PPCM remain to be determined. Additional areas of future investigation may include the delineation of genotype-phenotype correlations and the screening of newly-identified cardiomyopathy genes for their roles in PPCM. Nevertheless, these findings suggest that the construction of a family history may be advised in the management of PPCM and that genetic testing should be considered. A better understanding of the genetics of PPCM holds the potential to improve treatment, prognosis, and family management.
Physical Activity, Sedentary Behavior, Sleep and Self-Regulation in Spanish Preschoolers during the COVID-19 Lockdown
Authors: Alonso-Martínez Alicia M. , Ramírez-Vélez Robinson , García-Alonso Yesenia , Izquierdo Mikel , García-Hermoso Antonio
Published: January 2021

Background: A better understanding of the effects of the lockdown on lifestyle behaviors may help to guide the public health response to COVID-19 at a national level and to update the global strategy to respond COVID-19 pandemic. The aim of the study was to examine the effects of the COVID-19 lockdown on device-measured physical activity (PA), sedentary time, sleep and self-regulation; and to determine whether PA and sleep are related to self-regulation problems during the lockdown. Methods: PA, sedentary time and sleep were assessed using accelerometry in the week in which the Spanish national state of alarm was declared (n = 21). Parents reported preschooler’s self-regulation difficulties (internalizing and externalizing) before (n = 268) and during the lockdown (n = 157) by a validated questionnaire. Results: Preschoolers showed a decrease in total PA (mean difference [MD] = −43.3 min per day, 95% CI −68.1 to −18.5), sleep efficiency (MD = −2.09%, 95% CI −4.12 to −0.05), an increase in sedentary time (MD = 50.2 min per day, 95% CI 17.1 to 83.3) internalizing (MD = 0.17, 95% CI 0.06 to 0.28) and externalizing (MD = 0.33, 95% CI 0.23 to 0.44) problems. Preschoolers who met the World Health Organization recommendations for PA had lower internalizing scores than non-active peers (MD = −1.28, 95% CI −2.53 to −0.03). Conclusions: Our findings highlight the importance of meeting PA recommendations to reduce psychosocial difficulties during a lockdown situation.
Influence of Fatigue on Some Kinematic Parameters of Basketball Passing
Authors: Li Feng , Knjaz Damir , Rupčić Tomislav
Published: January 2021

Kinematic analysis is an objective method for examining basketball technique. However, there are just a few studies featuring a kinematic analysis of passing. The purpose of this study was to determine whether the kinematic parameters and accuracy of passing changed when players were under the influence of fatigue. Eleven Croatian basketball players who are members of the youth national program (age: 18.36 ± 0.67 years; height: 192.32 ± 9.98 cm; weight: 83.35 ± 11.19 kg; body fat: 15.00 ± 4.40%, arm span: 194.34 ± 10.39 cm) participated in fatigue and non-fatigue repetitive tests. A Xsens suit was used to analyze the kinematic parameters of push passing; a radar gun was used to determine ball speed; heart rate and blood lactate were used to identify fatigue and non-fatigue state. There was a significant difference in angular velocities of shoulder (p = 0.01), elbow (p = 0.04), and wrist (p = 0.01), accuracy (p = 0.01), ball speed (p = 0.00), pelvis position (p = 0.00), and velocity of the pelvis in X-axis (p = 0.00) between fatigue and non-fatigue state. Fatigue influences some kinematic parameters and accuracy of passing. The findings of this study suggest that coaches conduct as many drills as possible in situational conditions that are similar to the conditions during the basketball game itself.
Occupational Therapy and the Use of Technology on Older Adult Fall Prevention: A Scoping Review
Authors: Miranda-Duro María del Carmen , Nieto-Riveiro Laura , Concheiro-Moscoso Patricia , Groba Betania , Pousada Thais , Canosa Nereida , Pereira Javier
Published: January 2021

Introduction: Falls are the second leading cause of accidental or non-intentional deaths worldwide and are the most common problem as people age. The primary purpose of addressing falls is to detect, prevent, treat, and reduce their incidence and consequences. Previous studies identified that multifactorial programs, an interprofessional team, and assistive technology are required to address falls in older adults effectively. Accordingly, the research question is as follows: what are the scope, type of studies, and approaches and strategies to fall risk using technology in the existing occupational therapy literature regarding interventions to address the effects of falls in older adults on daily living? Methods: This scoping review was carried out in January 2020 through Biblioteca Virtual de Salud España, C.I.N.A.H.L., Cochrane Plus, OTSeeker, PubMed, Scopus, and Web of Science. Results: Twelve papers were included. We analyzed the year and journal of publication, authors’ affiliation, and design of the study, and thematic categories. There were three themes: participants’ characteristics, type of intervention, and fall approach and type of technology used. Discussion and Conclusions: The literature obtained is scarce. It is considered to still be an emerging theme, especially when considering the use of technology for occupational therapy.
The Impact of Planting Industry Structural Changes on Carbon Emissions in the Three Northeast Provinces of China
Authors: Guo Hongpeng , Xie Sidong , Pan Chulin
Published: January 2021

This paper focuses on the impact of changes in planting industry structure on carbon emissions. Based on the statistical data of the planting industry in three provinces in Northeast China from 1999 to 2018, the study calculated the carbon emissions, carbon absorptions and net carbon sinks of the planting industry by using crop parameter estimation and carbon emissions inventory estimation methods. In addition, the multiple linear regression model and panel data model were used to analyze and test the carbon emissions and net carbon sinks of the planting industry. The results show that: (1). The increase of the planting area of rice, corn, and peanuts in the three northeastern provinces of China will promote carbon emissions, while the increase of the planting area of wheat, sorghum, soybeans, and vegetables will reduce carbon emissions; (2). Fertilizer application, technological progress, and planting structure factors have a significant positive effect on net carbon sinks, among which the changes in the planting industry structure have the greatest impact on net carbon sinks. Based on the comprehensive analysis, it is suggested that, under the guidance of the government, resource endowment and location advantages should be given full play to, and the internal planting structure of crops should be reasonably adjusted so as to promote the development of low-carbon agriculture and accelerate the development process of agricultural modernization.
Psychological Distress and Post-Traumatic Symptomatology among Dental Healthcare Workers in Russia: Results of a Pilot Study
Authors: Sarapultseva Maria , Zolotareva Alena , Kritsky Igor , Nasretdinova Natal’ya , Sarapultsev Alexey
Published: January 2021

The spread of SARS-CoV-2 infection has increased the risk of mental health problems, including post-traumatic stress disorders (PTSD), and healthcare workers (HCWs) are at greater risk than other occupational groups. This observational cross-sectional study aimed to explore the symptoms of depression, anxiety, and PTSD among dental HCWs in Russia during the coronavirus disease 2019 (COVID-19) pandemic. The survey was carried out among 128 dental HCWs from three dental clinics of Ekaterinburg, Russia. The mean age of the sample was 38.6 years. Depression, anxiety, and stress were assessed using the Depression Anxiety and Stress Scale-21 (DASS-21); PTSD was assessed using the PTSD Symptom Scale-Self-Report (PSS-SR); subjective distress was assessed using the Impact of Event Scale-Revised (IES-R). The results indicated that 20.3–24.2% HCWs had mild to extremely severe symptoms of psychological distress, and 7.1–29.7% had clinical symptoms of PTSD. No differences between females and males were revealed. HCWs working directly with patients had significantly higher levels of PTSD symptoms and the risk of PTSD development compared to those working indirectly, whereas older HCWs had significantly higher levels of both psychological distress and PTSD symptoms compared to younger HCWs. Thus, dental HCWs are at high risk for psychological distress and PTSD symptoms during the COVID-19 pandemic.
Untargeted Urinary Metabolomics and Children’s Exposure to Secondhand Smoke: The Influence of Individual Differences
Authors: Zhu Huiwei , Abdullah Abu S. , He Jingyi , Xi Jianxiong , Mao Yimeng , Feng Yitian , Xiao Qianyi , Zheng Pinpin
Published: January 2021

Children’s exposure to secondhand smoke (SHS) is a severe public health problem. There is still a lack of evidence regarding panoramic changes in children’s urinary metabolites induced by their involuntary exposure to SHS, and few studies have considered individual differences. This study aims to clarify the SHS-induced changes in urinary metabolites in preschool children by using cross-sectional and longitudinal metabolomics analyses. Urinary metabolites were quantified by using untargeted ultra high-performance liquid chromatography-mass spectrometry (UPLC(c)-MS/MS). Urine cotinine-measured SHS exposure was examined to determine the exposure level. A cross-sectional study including 17 children in a low-exposure group, 17 in a medium-exposure group, and 17 in a high-exposure group was first conducted. Then, a before–after study in the cohort of children was carried out before and two months after smoking-cessation intervention for family smokers. A total of 43 metabolites were discovered to be related to SHS exposure in children in the cross-sectional analysis (false discovery rate (FDR) corrected p < 0.05, variable importance in the projection (VIP) > 1.0). Only three metabolites were confirmed to be positively associated with children’s exposure to SHS (FDR corrected p < 0.05) in a follow-up longitudinal analysis, including kynurenine, tyrosyl-tryptophan, and 1-(3-pyridinyl)-1,4-butanediol, the latter of which belongs to carbonyl compounds, peptides, and pyridines. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that 1-(3-pyridinyl)-1,4-butanediol and kynurenine were significantly enriched in xenobiotic metabolism by cytochrome P450 (p = 0.040) and tryptophan metabolism (p = 0.030), respectively. These findings provide new insights into the pathophysiological mechanism of SHS and indicate the influence of individual differences in SHS-induced changes in urinary metabolites in children.
The Association between Symptoms of Nomophobia, Insomnia and Food Addiction among Young Adults: Findings of an Exploratory Cross-Sectional Survey
Authors: Jahrami Haitham , Abdelaziz Ammar , Binsanad Latifa , Alhaj Omar A. , Buheji Mohammed , Bragazzi Nicola Luigi , Saif Zahra , BaHammam Ahmed S. , Vitiello Michael V.
Published: January 2021

No previous research has examined the association between symptoms of nomophobia and food addiction. Similarly, only a few studies have examined the association between nomophobia and symptoms of insomnia. This exploratory study utilized an online self-administered, structured questionnaire that included: basic sociodemographic and anthropometrics; the nomophobia questionnaire (NMP-Q); the insomnia severity index (ISI); and the Yale Food Addiction Scale (YFAS) in a convenience sample of young adults (18–35 years) in Bahrain (n = 654), 304 (46%) males and 350 (54%) females. Symptoms of severe nomophobia, moderate-severe insomnia, and food addiction were more common among female participants both for each disorder separately and in combination; however, differences did not reach statistical significance. For severe nomophobia, the rate for females was 76 (21.7%) and for males was 57 (18.8%) p = 0.9. For moderate-severe insomnia, the rate for females was 56 (16%) and for males was 36 (11.84%) p = 0.1. For food addiction, the rate for females was 71 (20.29%) and for males was 53 (17.43%) p = 0.3. A statistically significant association was present between nomophobia and insomnia r = 0.60, p < 0.001. No association was found between nomophobia and food addiction. Nomophobia is very common in young adults, particularly in females; nomophobia is associated with insomnia but not with food addiction.
Indoor Formaldehyde Concentration, Personal Formaldehyde Exposure and Clinical Symptoms during Anatomy Dissection Sessions, University of Medicine 1, Yangon
Authors: Aung Win-Yu , Sakamoto Hironari , Sato Ayana , Yi Ei-Ei-Pan-Nu , Thein Zaw-Lin , Nwe Myint-San , Shein Nanda , Linn Htin , Uchiyama Shigehisa , Kunugita Naoki , Win-Shwe Tin-Tin , Mar Ohn
Published: January 2021

The formaldehyde (FA) embalming method, the world’s most common protocol for the fixation of cadavers, has been consistently used in medical universities in Myanmar. This study was designed to examine the indoor FA concentrations in anatomy dissection rooms, an exposed site, and lecture theater, an unexposed control site, and to access personal exposure levels of FA and clinical symptoms of medical students and instructors. In total, 208 second year medical students (1/2019 batch) and 18 instructors from Department of Anatomy, University of Medicine 1, participated. Thirteen dissection sessions were investigated from February 2019 to January 2020. Diffusive sampling devices were used as air samplers and high-performance liquid chromatography was used for measurement of FA. Average indoor FA concentration of four dissection rooms was 0.43 (0.09–1.22) ppm and all dissection rooms showed indoor concentrations above the occupational exposure limits and short-term exposure limit for general population. Personal FA exposure values were higher than indoor FA concentrations and the instructors (0.68, 0.04–2.11 ppm) had higher exposure than the students (0.44, 0.06–1.72 ppm). Unpleasant odor, eye and nose irritations and inability to concentrate were frequently reported FA-related symptoms, and the students were found to have significantly higher risks (p < 0.05) of having these symptoms during the dissection sessions than during lecture.
Acute Effects of Global Postural Re-Education on Non-Specific Low Back Pain. Does Time-of-Day Play a Role?
Authors: Merinero David , Rodríguez-Aragón Manuel , Álvarez-González Javier , López-Samanes Álvaro , López-Pascual Joaquín
Published: January 2021

Low back pain is one the most common forms of musculoskeletal disorders. Thus, several physiotherapeutic strategies (e.g., global postural re-education therapy) have been used for reducing low back pain. The aim of this study was to determinate if acute application of global postural re-education session associated effects are influenced by the time-of-day when this physical therapy is applied. Eight participants in a randomized, counterbalanced order were acutely tested both before and 24 h after a global postural re-education therapy session (10 min session) in three different time-of-day points; morning (i.e., AM; 7:00–9:00 h), midday (i.e., AM; 12:00–14:00 h) and afternoon (i.e., PM; 18:00–20:00 h). In each session, low back pain Visual Analogue Pain Scale [VAS]), flexibility, function capacity (Roland Morris Questionnaire [RMQ], and physical functioning Oswestry Disability Index [ODI]) were recorded. Results showed a pain reduction (VAS Scale) 24 h post Global postural re-education [GPR] session (p = 0.001) and increasing of flexibility pre-post GPR session in all the time-of-day points (morning, midday, and afternoon) (p = 0.001) while no differences were reported in RMQ (p = 0.969) and ODI (p = 0.767). Thus, acute GPR session produces the same effects on flexibility, low back pain, function capacity, and physical functioning values independently of time-of-day when it is applied.
Detrimental Health Behaviour Changes among Females Living in Rural Areas during the COVID-19 Pandemic
Authors: Glenister Kristen M. , Ervin Kaye , Podubinski Tegan
Published: January 2021

Women are predicted to be disproportionately impacted by the COVID-19 pandemic due to increased carer responsibilities, loss of income, worry about the virus and a predominantly female healthcare workforce. Whilst there is emerging evidence that negative mental health impacts associated with the COVID-19 pandemic may be more pronounced for women than men, less attention has focussed on changes to health behaviours and health seeking experienced by women. Similarly, the impact of the pandemic in rural areas has not been investigated in detail. Our research questions were ‘have females residing in rural areas experienced changes in alcohol consumption, unhealthy food consumption, smoking, exercise or health seeking during the COVID-19 pandemic?’ and ‘are there differences in health behaviour changes between rural females living with or without children?’. Net increases (scale of 0–1) in consumption of unhealthy food (95% CI 0.05, 0.22) and alcohol (95% CI 0.12, 0.29) were observed. Net decreases (scale of −1 to 0) in visits to the doctor (95% CI −0.23, −0.35) and other health professionals (95% CI −0.40, −0.54) were observed. Compared with females living without children, females who lived with children were significantly associated with increased alcohol consumption (OR 2.4 (95% CI 1.4, 4.1), decreased visits to the doctor (OR 1.9 (95% CI 1.1, 3.2) and decreased visits to other health professionals (OR 1.9 (95% CI 1.1, 3.3). Results suggest that public health approaches may be required to support females residing in rural areas to optimise their health behaviours during the pandemic, particularly for those living with children. Policies must be gender responsive to counteract worsening health and social inequities both during and after the pandemic.
PPAR Gamma: From Definition to Molecular Targets and Therapy of Lung Diseases
Authors: Carvalho Márcia V. de , Gonçalves-de-Albuquerque Cassiano F. , Silva Adriana R.
Published: January 2021

Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear receptor superfamily that regulate the expression of genes related to lipid and glucose metabolism and inflammation. There are three members: PPARα, PPARβ or PPARγ. PPARγ have several ligands. The natural agonists are omega 9, curcumin, eicosanoids and others. Among the synthetic ligands, we highlight the thiazolidinediones, clinically used as an antidiabetic. Many of these studies involve natural or synthetic products in different pathologies. The mechanisms that regulate PPARγ involve post-translational modifications, such as phosphorylation, sumoylation and ubiquitination, among others. It is known that anti-inflammatory mechanisms involve the inhibition of other transcription factors, such as nuclear factor kB(NFκB), signal transducer and activator of transcription (STAT) or activator protein 1 (AP-1), or intracellular signaling proteins such as mitogen-activated protein (MAP) kinases. PPARγ transrepresses other transcription factors and consequently inhibits gene expression of inflammatory mediators, known as biomarkers for morbidity and mortality, leading to control of the exacerbated inflammation that occurs, for instance, in lung injury/acute respiratory distress. Many studies have shown the therapeutic potentials of PPARγ on pulmonary diseases. Herein, we describe activities of the PPARγ as a modulator of inflammation, focusing on lung injury and including definition and mechanisms of regulation, biological effects and molecular targets, and its role in lung diseases caused by inflammatory stimuli, bacteria and virus, and molecular-based therapy.
Optogenetic Stimulation of Prelimbic Pyramidal Neurons Maintains Fear Memories and Modulates Amygdala Pyramidal Neuron Transcriptome
Authors: Laricchiuta Daniela , Sciamanna Giuseppe , Gimenez Juliette , Termine Andrea , Fabrizio Carlo , Caioli Silvia , Balsamo Francesca , Panuccio Anna , De Bardi Marco , Saba Luana , Passarello Noemi , Cutuli Debora , Mattioni Anna , Zona Cristina , Orlando Valerio , Petrosini Laura
Published: January 2021

Fear extinction requires coordinated neural activity within the amygdala and medial prefrontal cortex (mPFC). Any behavior has a transcriptomic signature that is modified by environmental experiences, and specific genes are involved in functional plasticity and synaptic wiring during fear extinction. Here, we investigated the effects of optogenetic manipulations of prelimbic (PrL) pyramidal neurons and amygdala gene expression to analyze the specific transcriptional pathways associated to adaptive and maladaptive fear extinction. To this aim, transgenic mice were (or not) fear-conditioned and during the extinction phase they received optogenetic (or sham) stimulations over photo-activable PrL pyramidal neurons. At the end of behavioral testing, electrophysiological (neural cellular excitability and Excitatory Post-Synaptic Currents) and morphological (spinogenesis) correlates were evaluated in the PrL pyramidal neurons. Furthermore, transcriptomic cell-specific RNA-analyses (differential gene expression profiling and functional enrichment analyses) were performed in amygdala pyramidal neurons. Our results show that the optogenetic activation of PrL pyramidal neurons in fear-conditioned mice induces fear extinction deficits, reflected in an increase of cellular excitability, excitatory neurotransmission, and spinogenesis of PrL pyramidal neurons, and associated to strong modifications of the transcriptome of amygdala pyramidal neurons. Understanding the electrophysiological, morphological, and transcriptomic architecture of fear extinction may facilitate the comprehension of fear-related disorders.
Computational Evolution of Beta-2-Microglobulin Binding Peptides for Nanopatterned Surface Sensors
Authors: Adedeji Olulana Abimbola Feyisara , Soler Miguel A. , Lotteri Martina , Vondracek Hendrik , Casalis Loredana , Marasco Daniela , Castronovo Matteo , Fortuna Sara
Published: January 2021

The bottom-up design of smart nanodevices largely depends on the accuracy by which each of the inherent nanometric components can be functionally designed with predictive methods. Here, we present a rationally designed, self-assembled nanochip capable of capturing a target protein by means of pre-selected binding sites. The sensing elements comprise computationally evolved peptides, designed to target an arbitrarily selected binding site on the surface of beta-2-Microglobulin (β2m), a globular protein that lacks well-defined pockets. The nanopatterned surface was generated by an atomic force microscopy (AFM)-based, tip force-driven nanolithography technique termed nanografting to construct laterally confined self-assembled nanopatches of single stranded (ss)DNA. These were subsequently associated with an ssDNA–peptide conjugate by means of DNA-directed immobilization, therefore allowing control of the peptide’s spatial orientation. We characterized the sensitivity of such peptide-containing systems against β2m in solution by means of AFM-based differential topographic imaging and surface plasmon resonance (SPR) spectroscopy. Our results show that the confined peptides are capable of specifically capturing β2m from the surface–liquid interface with micromolar affinity, hence providing a viable proof-of-concept for our approach to peptide design.
Osteosarcoma Pathogenesis Leads the Way to New Target Treatments
Authors: Fernandes Isabel , Melo-Alvim Cecília , Lopes-Brás Raquel , Esperança-Martins Miguel , Costa Luís
Published: January 2021

Osteosarcoma (OS) is a rare condition with very poor prognosis in a metastatic setting. Basic research has enabled a better understanding of OS pathogenesis and the discovery of new potential therapeutic targets. Phase I and II clinical trials are already ongoing, with some promising results for these patients. This article reviews OS pathogenesis and new potential therapeutic targets.
Toll-Like Receptors in Acute Kidney Injury
Authors: Vázquez-Carballo Cristina , Guerrero-Hue Melania , García-Caballero Cristina , Rayego-Mateos Sandra , Opazo-Ríos Lucas , Morgado-Pascual José Luis , Herencia-Bellido Carmen , Vallejo-Mudarra Mercedes , Cortegano Isabel , Gaspar María Luisa , de Andrés Belén , Egido Jesús , Moreno Juan Antonio
Published: January 2021

Acute kidney injury (AKI) is an important health problem, affecting 13.3 million individuals/year. It is associated with increased mortality, mainly in low- and middle-income countries, where renal replacement therapy is limited. Moreover, survivors show adverse long-term outcomes, including increased risk of developing recurrent AKI bouts, cardiovascular events, and chronic kidney disease. However, there are no specific treatments to decrease the adverse consequences of AKI. Epidemiological and preclinical studies show the pathological role of inflammation in AKI, not only at the acute phase but also in the progression to chronic kidney disease. Toll-like receptors (TLRs) are key regulators of the inflammatory response and have been associated to many cellular processes activated during AKI. For that reason, a number of anti-inflammatory agents targeting TLRs have been analyzed in preclinical studies to decrease renal damage during AKI. In this review, we updated recent knowledge about the role of TLRs, mainly TLR4, in the initiation and development of AKI as well as novel compounds targeting these molecules to diminish kidney injury associated to this pathological condition.
Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine
Authors: La Hyeonwoo , Yoo Hyunjin , Lee Eun Joo , Thang Nguyen Xuan , Choi Hee Jin , Oh Jeongheon , Park Ji Hyun , Hong Kwonho
Published: January 2021

Mechanistic understanding of germ cell formation at a genome-scale level can aid in developing novel therapeutic strategies for infertility. Germ cell formation is a complex process that is regulated by various mechanisms, including epigenetic regulation, germ cell-specific gene transcription, and meiosis. Gonads contain a limited number of germ cells at various stages of differentiation. Hence, genome-scale analysis of germ cells at the single-cell level is challenging. Conventional genome-scale approaches cannot delineate the landscape of genomic, transcriptomic, and epigenomic diversity or heterogeneity in the differentiating germ cells of gonads. Recent advances in single-cell genomic techniques along with single-cell isolation methods, such as microfluidics and fluorescence-activated cell sorting, have helped elucidate the mechanisms underlying germ cell development and reproductive disorders in humans. In this review, the history of single-cell transcriptomic analysis and their technical advantages over the conventional methods have been discussed. Additionally, recent applications of single-cell transcriptomic analysis for analyzing germ cells have been summarized.
Potentiometric Study of Carbon Nanotube/Surfactant Interactions by Ion-Selective Electrodes. Driving Forces in the Adsorption and Dispersion Processes
Authors: Ostos Francisco José , Lebrón José Antonio , Moyá María Luisa , Bernal Eva , Flores Ana , Lépori Cristian , Maestre Ángeles , Sánchez Francisco , López-Cornejo Pilar , López-López Manuel
Published: January 2021

The interaction (adsorption process) of commercial ionic surfactants with non-functionalized and functionalized carbon nanotubes (CNTs) has been studied by potentiometric measurements based on the use of ion-selective electrodes. The goal of this work was to investigate the role of the CNTs’ charge and structure in the CNT/surfactant interactions. Non-functionalized single- (SWCNT) and multi-walled carbon nanotubes (MWCNT), and amine functionalized SWCNT were used. The influence of the surfactant architecture on the CNT/surfactant interactions was also studied. Surfactants with different charge and hydrophobic tail length (sodium dodecyl sulfate (SDS), octyltrimethyl ammonium bromide (OTAB), dodecyltrimethyl ammonium bromide (DoTAB) and hexadecyltrimethyl ammonium bromide (CTAB)) were studied. According to the results, the adsorption process shows a cooperative character, with the hydrophobic interaction contribution playing a key role. This is made evident by the correlation between the free surfactant concentration (at a fixed [CNT]) and the critical micellar concentration, cmc, found for all the CNTs and surfactants investigated. The electrostatic interactions mainly determine the CNT dispersion, although hydrophobic interactions also contribute to this process.