bluuebunny/crossref_metadata_2025 huggingface dataset:
A cluster randomised control trial of a multi-component weight management programme for adults with intellectual disabilities and obesity
Authors: Harris Leanne , Hankey Catherine , Jones Nathalie , Pert Carol , Murray Heather , Tobin Janet , Boyle Susan , Melville Craig
Published: August 2017

There have been few published controlled studies of multi-component weight management programmes that include an energy deficit diet (EDD), for adults with intellectual disabilities and obesity. The objective of this study was to conduct a single-blind, cluster randomised controlled trial comparing a multi-component weight management programme to a health education programme. Participants were randomised to either TAKE 5, which included an EDD or Waist Winners Too (WWToo), based on health education principles. Outcomes measured at baseline, 6 months (after a weight loss phase) and 12 months (after a 6-month weight maintenance phase), by a researcher blinded to treatment allocation, included: weight; BMI; waist circumference; physical activity; sedentary behaviour and health-related quality of life. The recruitment strategy was effective with fifty participants successfully recruited. Both programmes were acceptable to adults with intellectual disabilities, evidenced by high retention rates (90 %). Exploratory efficacy analysis revealed that at 12 months there was a trend for more participants in TAKE 5 (50·0 %) to achieve a clinically important weight loss of 5–10 %, in comparison to WWToo (20·8 %) (OR 3·76; 95 % CI 0·92, 15·30; 0·064). This study found that a multi-component weight management programme that included an EDD, is feasible and an acceptable approach to weight loss when tailored to meet the needs of adults with intellectual disabilities and obesity.
Processed red meat contribution to dietary patterns and the associated cardio-metabolic outcomes
Authors: Lenighan Yvonne M. , Nugent Anne P. , Li Kaifeng F. , Brennan Lorraine , Walton Janette , Flynn Albert , Roche Helen M. , McNulty Breige A.
Published: August 2017

Evidence suggests that processed red meat consumption is a risk factor for CVD and type 2 diabetes (T2D). This analysis investigates the association between dietary patterns, their processed red meat contributions, and association with blood biomarkers of CVD and T2D, in 786 Irish adults (18–90 years) using cross-sectional data from a 2011 national food consumption survey. All meat-containing foods consumed were assigned to four food groups (n 502) on the basis of whether they contained red or white meat and whether they were processed or unprocessed. The remaining foods (n 2050) were assigned to twenty-nine food groups. Two-step and k-means cluster analyses were applied to derive dietary patterns. Nutrient intakes, plasma fatty acids and biomarkers of CVD and T2D were assessed. A total of four dietary patterns were derived. In comparison with the pattern with lower contributions from processed red meat, the dietary pattern with greater processed red meat intakes presented a poorer Alternate Healthy Eating Index (21·2 (sd 7·7)), a greater proportion of smokers (29 %) and lower plasma EPA (1·34 (sd 0·72) %) and DHA (2·21 (sd 0·84) %) levels (P<0·001). There were no differences in classical biomarkers of CVD and T2D, including serum cholesterol and insulin, across dietary patterns. This suggests that the consideration of processed red meat consumption as a risk factor for CVD and T2D may need to be re-assessed.
A comparative analysis of vehicle-related greenhouse gas emissions between organic and conventional dairy production
Authors: Aggestam Vivianne , Buick Jon
Published: August 2017

Agricultural industrialisation and globalisation have steadily increased the transportation of food across the world. In efforts to promote sustainability and self-sufficiency, organic milk producers in Sweden are required to produce a higher level of cattle feed on-farm in the hope that increased self-sufficiency will reduce reliance on external inputs and reduce transport-related greenhouse gas emissions. Using data collected from 20 conventional and 20 organic milk producers in Sweden this paper aims to assess the global warming impact of farmyard vehicles and the transportation of feed produced ‘off-farm’ in order to compare the impact of vehicle-related emissions from the different production methods. The findings show organic and conventional production methods have different vehicle-related emission outputs that vary according to a reliance on either road transportation or increased farmyard machinery use. Mechanical weeding is more fuel demanding than conventional agrichemical sprayers. However, artificial fertilising is one of the highest farmyard vehicle-related emitters. The general findings show organic milk production emits higher levels of farm vehicle-related emissions that fail to be offset by reduced emissions occurring from international transport emissions. This paper does not propose to cover a comprehensive supply chain carbon footprint for milk production or attempt to determine which method of production has the largest climatic impact. However, it does demonstrate that Sweden's legal requirements for organic producers to produce more feed on-farm to reduce transport emissions have brought emissions back within Sweden's greenhouse gas inventory and raises questions around the effectiveness of policies to reduce vehicle-related emissions. Further research is needed into the effectiveness of climate change mitigation on food production policies, in particular looking at various trade-offs that affects the entire food supply chain.
Biological activity of the non-microbial fraction of kefir: antagonism against intestinal pathogens
Authors: Iraporda Carolina , Abatemarco Júnior Mário , Neumann Elisabeth , Nunes Álvaro Cantini , Nicoli Jacques R , Abraham Analía G , Garrote Graciela L.
Published: August 2017

Kefir is a fermented milk obtained by the activity of kefir grains which are composed of lactic and acetic acid bacteria, and yeasts. Many beneficial health effects have been associated with kefir consumption such as stimulation of the immune system and inhibition of pathogenic microorganisms. The biological activity of kefir may be attributed to the presence of a complex microbiota as well as the microbial metabolites that are released during fermentation. The aim of this work was to characterise the non-microbial fraction of kefir and to study its antagonism againstEscherichia coli,Salmonellaspp. andBacillus cereus.During milk fermentation there was a production of organic acids, mainly lactic and acetic acid, with a consequent decrease in pH and lactose content. The non-microbial fraction of kefir added to nutrient broth at concentrations above 75% v/v induced a complete inhibition of pathogenic growth that could be ascribed to the presence of un-dissociated lactic acid. In vitro assays using an intestinal epithelial cell model indicated that pre-incubation of cells with the non-microbial fraction of kefir did not modify the association/invasion ofSalmonellawhereas pre-incubation ofSalmonellawith this fraction under conditions that did not affect their viability significantly decreased the pathogen's ability to invade epithelial cells. Lactate exerted a protective effect againstSalmonellain a mouse model, demonstrating the relevance of metabolites present in the non-microbial fraction of kefir produced during milk fermentation.
Capacity of milk composition to identify the feeding system used to feed dairy cows
Authors: Vicente Fernando , Santiago Carme , Jiménez-Calderón José D , Martínez-Fernández Adela
Published: August 2017

This Research Paper addresses the hypothesis that is possible to identify the type of feed used for dairy cows by means of the analysis of milk composition and the fatty acid profile of milk fat. Sixteen dairy farms were monitored during 1 year with quarterly visits between summer 2014 and spring 2015. Rations varied throughout the year due to annual dynamic change of forage production, forage rotation, variation of nutrient requirements according to physiological state of the animal, etc. The ingredients of the rations were analysed by cluster identifying five feeding systems based on the main ingredient of the diet: grazing, maize silage, grass silage, dry forage and concentrate. Milk composition could explain up to 91·3% of the total variability among feeding systems, while fatty acid profile could explain only up to 61·2% of total variability. However, when the sum of types of fatty acids and their ratios are taken, up to 93·5% of total variability could be explained. The maize silage system had the greatest milk yield, protein, solid non-fat and urea proportions, as well as the highest proportion of saturated fatty acid and lowest concentration of trans11 18 : 1, cis9 18 : 1 and 18 : 3n3. Principal component analysis distinguishes the maize silage system from other feeding systems, both from milk composition and milk fatty acid profile. Concentrate system overlapped partially with the grazing, grass silage and dry forage systems. The latter systems had the highest concentrations of cis9 18 : 1, trans11 18 : 1 and 18 : 3, but there was no clear differentiation among them.
Statistical properties of proportional residual energy intake as a new measure of energetic efficiency
Authors: Zamani Pouya
Published: August 2017

Traditional ratio measures of efficiency, including feed conversion ratio (FCR), gross milk efficiency (GME), gross energy efficiency (GEE) and net energy efficiency (NEE) may have some statistical problems including high correlations with milk yield. Residual energy intake (REI) or residual feed intake (RFI) is another criterion, proposed to overcome the problems attributed to the traditional ratio criteria, but it does not account for production or intake levels. For example, the same REI value could be considerable for low producing and negligible for high producing cows. The aim of this study was to propose a new measure of efficiency to overcome the problems attributed to the previous criteria. A total of 1478 monthly records of 268 lactating Holstein cows were used for this study. In addition to FCR, GME, GEE, NEE and REI, a new criterion called proportional residual energy intake (PREI) was calculated as REI to net energy intake ratio and defined as proportion of net energy intake lost as REI. The PREI had an average of −0·02 and range of −0·36 to 0·27, meaning that the least efficient cow lost 0·27 of her net energy intake as REI, while the most efficient animal saved 0·36 of her net energy intake as less REI. Traditional ratio criteria (FCR, GME, GEE and NEE) had high correlations with milk and fat corrected milk yields (absolute values from 0·469 to 0·816), while the REI and PREI had low correlations (0·000 to 0·069) with milk production. The results showed that the traditional ratio criteria (FCR, GME, GEE and NEE) are highly influenced by production traits, while the REI and PREI are independent of production level. Moreover, the PREI adjusts the REI magnitude for intake level. It seems that the PREI could be considered as a worthwhile measure of efficiency for future studies.
Influence of milk processing temperature on growth performance, nitrogen retention, and hindgut's inflammatory status and bacterial populations in a calf model
Authors: Bach Alex , Aris Anna , Vidal Maria , Fàbregas Francesc , Terré Marta
Published: August 2017

This research communication describes a study aimed at evaluating the effects of heat treatment of milk on growth performance, N retention, and hindgut's inflammatory status and bacterial populations using young dairy calves as a model. Twenty-one Holstein calves were randomly allocated to one of three treatments: raw milk (RM), pasteurised milk (PAST), or UHT milk (UHT). Calves were submitted to a N balance study, and a biopsy from the distal colon and a faecal sample were obtained from 5 animals per treatment to determine expression of several genes and potential changes in the hindgut's bacterial population. Milk furosine content was 33-fold greater in UHT than in RM and PAST milks. Calves receiving RM grew more than those fed UHT, and urinary N excretion was greatest in calves fed UHT. Quantification of Lactobacillus was lower in calves consuming PAST or UHT, and Gram negative bacteria were greater in UHT than in PAST calves. The expression of IL-8 in the hindgut's mucosa was lowest and that of IL-10 tended to be lowest in RM calves, and expression of claudin-4 tended to be greatest in UHT calves. In conclusion, the nutritional value of UHT-treated milk may be hampered because it compromises growth and increases N excretion in young calves and may have deleterious effects on the gut's bacterial population and inflammation status.
Analysis of microsatellite markers in a Cuban water buffalo breed
Authors: Uffo Odalys , Martínez Nadia , Acosta Atzel , Sanz Arianne , Martín-Burriel Inmaculada , Osta Rosario , Rodellar Clementina , Zaragoza Pilar
Published: August 2017

The aim of this Regional Research Communication was to validate a panel of 30 microsatellite markers recommended by FAO/ISAG for studies of biodiversity in cattle to improve the characterisation of Cuban buffalo populations. The water buffalo (Bubalus bubalis) is an economically important livestock species. Therefore, research focused on the study of the genetic relationships among water buffalo populations is useful to support conservation decisions and to design breeding schemes. Twenty-eight of the 30 tested regions were amplified, one of which (ETH10) turned out to be monomorphic. A total of 143 alleles were observed in the Cuban water buffalo population. The average number of alleles per locus was 5·04. The number of alleles per polymorphic locus ranged from two (INRA 63 and MM12) to nine (ETH185). The observed and expected heterozygosity ranged from 0·108 (HAUT24) to 0·851 (CSSM66) and 0·104 (MM12) to 0·829(INRA32), respectively. The polymorphic information content (PIC) ranged from 0·097 (MM12) to 0·806 (INRA32), and the overall value for these markers was 0·482. Within the population, inbreeding estimates (FIS) was positive in 14 of the 30 loci analysed. This study thus highlights the usefulness of heterologous bovine microsatellite markers to assess the genetic variability in Cuban water buffalo breeds. Furthermore, the results can be utilised for future breeding strategies and conservation.
Expression profiles of miRNAs from bovine mammary glands in response to Streptococcus agalactiae-induced mastitis
Authors: Pu Junhua , Li Rui , Zhang Chenglong , Chen Dan , Liao Xiangxiang , Zhu Yihui , Geng Xiaohan , Ji Dejun , Mao Yongjiang , Gong Yunchen , Yang Zhangping
Published: August 2017

This study aimed to describe the expression profiles of microRNAs (miRNAs) from mammary gland tissues collected from dairy cows with Streptococcus agalactiae-induced mastitis and to identify differentially expressed miRNAs related to mastitis. The mammary glands of Chinese Holstein cows were challenged with Streptococcus agalactiae to induce mastitis. Small RNAs were isolated from the mammary tissues of the test and control groups and then sequenced using the Solexa sequencing technology to construct two small RNA libraries. Potential target genes of these differentially expressed miRNAs were predicted using the RNAhybrid software, and KEGG pathways associated with these genes were analysed. A total of 18 555 913 and 20 847 000 effective reads were obtained from the test and control groups, respectively. In total, 373 known and 399 novel miRNAs were detected in the test group, and 358 known and 232 novel miRNAs were uncovered in the control group. A total of 35 differentially expressed miRNAs were identified in the test group compared to the control group, including 10 up-regulated miRNAs and 25 down-regulated miRNAs. Of these miRNAs, miR-223 exhibited the highest degree of up-regulation with an approximately 3-fold increase in expression, whereas miR-26a exhibited the most decreased expression level (more than 2-fold). The RNAhybrid software predicted 18 801 genes as potential targets of these 35 miRNAs. Furthermore, several immune response and signal transduction pathways, including the RIG-I-like receptor signalling pathway, cytosolic DNA sensing pathway and Notch signal pathway, were enriched in these predicted targets. In summary, this study provided experimental evidence for the mechanism underlying the regulation of bovine mastitis by miRNAs and showed that miRNAs might be involved in signal pathways during S. agalactiae-induced mastitis.
Collisionless microinstabilities in stellarators. Part 4. The ion-driven trapped-electron mode
Authors: Plunk G. G. , Connor J. W. , Helander P.
Published: August 2017

Optimised stellarators and other magnetic-confinement devices having the property that the average magnetic curvature is favourable for all particle orbits are called maximum-$J$ devices. They have recently been shown to be immune to trapped-particle instabilities driven by the density gradient. Gyrokinetic simulations reveal, however, that another instability can arise, which is also associated with particle trapping but causes less transport than typical trapped-electron modes. The nature of this instability is clarified here. It is shown to be similar to the ‘ubiquitous mode’ in tokamaks and is driven by ion free energy, but requires trapped electrons to exist.
Palatal click allophony in Mangetti Dune !Xung: Implications for sound change
Authors: Miller Amanda L.
Published: August 2017

Click consonants are well known for lacking allophonic variation. This lack of variation has been attributed to the existence of articulatory constraints on the coronal constrictions that are imposed by the existence of a second dorsal constriction. The current study investigates temporal acoustic differences among the four contrastive coronal click types in the /i/ and /u/ contexts in Mangetti Dune !Xung. Clicks have been described as being either non-affricated or affricated. However, when vowel context is taken into consideration, the typology is more complex. The alveolar click is non-affricated in both vowel contexts. The dental and lateral clicks are fricated in both contexts. The palatal click in the /i/ context has two clear anterior and posterior transients, followed by palatal frication, while in the /u/ context it is non-affricated. Results are consistent with an analysis of the palatal click in the /i/ context as involving allophonic secondary palatalization. There are trading relations between the duration of the click burst, frication noise and aspiration noise phases. Results have implications for understanding the synchronic and diachronic phonology of click consonants.
Biological factors that impinge on Chagas disease drug development
Authors: FRANCISCO AMANDA F. , JAYAWARDHANA SHIROMANI , LEWIS MICHAEL D. , TAYLOR MARTIN C. , KELLY JOHN M.
Published: August 2017

Chagas disease is caused by infection with the insect-transmitted protozoan Trypanosoma cruzi, and is the most important parasitic infection in Latin America. The current drugs, benznidazole and nifurtimox, are characterized by limited efficacy and toxic side-effects, and treatment failures are frequently observed. The urgent need for new therapeutic approaches is being met by a combined effort from the academic and commercial sectors, together with major input from not-for-profit drug development consortia. With the disappointing outcomes of recent clinical trials against chronic Chagas disease, it has become clear that an incomplete understanding of parasite biology and disease pathogenesis is impacting negatively on the development of more effective drugs. In addition, technical issues, including difficulties in establishing parasitological cure in both human patients and animal models, have greatly complicated the assessment of drug efficacy. Here, we outline the major questions that need to be addressed and discuss technical innovations that can be exploited to accelerate the drug development pipeline.
Temperature-related parasite infection dynamics: the case of proliferative kidney disease of brown trout
Authors: STREPPARAVA NICOLE , SEGNER HELMUT , ROS ALBERT , HARTIKAINEN HANNA , SCHMIDT-POSTHAUS HEIKE , WAHLI THOMAS
Published: August 2017

Climate change, in particular rising temperature, is suspected to be a major driver for the emergence of many wildlife diseases. Proliferative kidney disease of salmonids, caused by the myxozoanTetracapsuloides bryosalmonae, was used to evaluate how temperature dependence of host–parasite interactions modulates disease emergence. Brown trout (Salmo trutta fario) kept at 12 and 15 °C, were experimentally infected withT. bryosalmonae. Parasite development in the fish host and release of spores were quantified simultaneously to unravel parasite transmission potential from the vertebrate to the invertebrate host. A change to a stable plateau in infection intensity of the kidney coincided with a threshold at which spore shedding commenced. This onset of parasite release was delayed at the low temperature in accordance with reaching this infection intensity threshold, but the amount of spores released was irrespective of temperature. The production of parasite transmission stages declined with time. In conclusion, elevated temperature modifies the parasite transmission opportunities by increasing the duration of transmission stage production, which may affect the spread and establishment of the parasite in a wider range of rivers.
Subliminal and supraliminal processing of reward-related stimuli in anorexia nervosa
Authors: Boehm I. , King J. A. , Bernardoni F. , Geisler D. , Seidel M. , Ritschel F. , Goschke T. , Haynes J.-D. , Roessner V. , Ehrlich S.
Published: August 2017

Background

Previous studies have highlighted the role of the brain reward and cognitive control systems in the etiology of anorexia nervosa (AN). In an attempt to disentangle the relative contribution of these systems to the disorder, we used functional magnetic resonance imaging (fMRI) to investigate hemodynamic responses to reward-related stimuli presented both subliminally and supraliminally in acutely underweight AN patients and age-matched healthy controls (HC).

Methods

fMRI data were collected from a total of 35 AN patients and 35 HC, while they passively viewed subliminally and supraliminally presented streams of food, positive social, and neutral stimuli. Activation patterns of the group×stimulation condition×stimulus type interaction were interrogated to investigate potential group differences in processing different stimulus types under the two stimulation conditions. Moreover, changes in functional connectivity were investigated using generalized psychophysiological interaction analysis.

Results

AN patients showed a generally increased response to supraliminally presented stimuli in the inferior frontal junction (IFJ), but no alterations within the reward system. Increased activation during supraliminal stimulation with food stimuli was observed in the AN group in visual regions including superior occipital gyrus and the fusiform gyrus/parahippocampal gyrus. No group difference was found with respect to the subliminal stimulation condition and functional connectivity.

Conclusion

Increased IFJ activation in AN during supraliminal stimulation may indicate hyperactive cognitive control, which resonates with clinical presentation of excessive self-control in AN patients. Increased activation to food stimuli in visual regions may be interpreted in light of an attentional food bias in AN.
Religion and the Regime
Authors: Koesel Karrie J.
Published: August 2017

What is the nature of religion and state relations in authoritarian regimes? How do religious and regime actors negotiate the terms of their relationship;what do the two sides want from one another; and how cooperative or conflictual are their interactions? To address these questions, the author compares religion-regime relations in contemporary Russia and China—two autocracies with long histories of religious repression, diverse religious profiles, and distinct relations between religion and the state. The article introduces a new theoretical framework anchored in interests and subnational authoritarian politics to explain how religious and political authorities negotiate their relationship and the constraints and opportunities that shape their interaction. Although there are many reasons to expect different types of religion-regime relations across Russia and China, the data demonstrate that subnational governments and diverse religious actors often forge innovative partnerships to govern more efficiently, gain access to resources, and safeguard their survival.
A new algorithm to automate inductive learning of default theories
Authors: SHAKERIN FARHAD , SALAZAR ELMER , GUPTA GOPAL
Published: August 2017

In inductive learning of a broad concept, an algorithm should be able to distinguish concept examples from exceptions and noisy data. An approach through recursively finding patterns in exceptions turns out to correspond to the problem of learning default theories. Default logic is what humans employ in common-sense reasoning. Therefore, learned default theories are better understood by humans. In this paper, we present new algorithms to learn default theories in the form of non-monotonic logic programs. Experiments reported in this paper show that our algorithms are a significant improvement over traditional approaches based on inductive logic programming. Under consideration for acceptance in TPLP.
The intricacies of three-valued extensional semantics for higher-order logic programs
Authors: RONDOGIANNIS PANOS , SYMEONIDOU IOANNA
Published: August 2017

M. Bezem defined an extensional semantics for positive higher-order logic programs. Recently, it was demonstrated by Rondogiannis and Symeonidou that Bezem's technique can be extended to higher-order logic programs with negation, retaining its extensional properties, provided that it is interpreted under a logic with an infinite number of truth values. Rondogiannis and Symeonidou also demonstrated that Bezem's technique, when extended under the stable model semantics, does not in general lead to extensional stable models. In this paper, we consider the problem of extending Bezem's technique under the well-founded semantics. We demonstrate that the well-founded extensionfailsto retain extensionality in the general case. On the positive side, we demonstrate that for stratified higher-order logic programs, extensionality is indeed achieved. We analyze the reasons of the failure of extensionality in the general case, arguing that a three-valued setting cannot distinguish between certain predicates that appear to have a different behaviour inside a program context, but which happen to be identical as three-valued relations.
Fixpoint semantics and optimization of recursive Datalog programs with aggregates
Authors: ZANIOLO CARLO , YANG MOHAN , DAS ARIYAM , SHKAPSKY ALEXANDER , CONDIE TYSON , INTERLANDI MATTEO
Published: August 2017

A very desirable Datalog extension investigated by many researchers in the last 30 years consists in allowing the use of the basic SQL aggregates min, max, count and sum in recursive rules. In this paper, we propose a simple comprehensive solution that extends the declarative least-fixpoint semantics of Horn Clauses, along with the optimization techniques used in the bottom-up implementation approach adopted by many Datalog systems. We start by identifying a large class of programs of great practical interest in which the use of min or max in recursive rules does not compromise the declarative fixpoint semantics of the programs using those rules. Then, we revisit the monotonic versions of count and sum aggregates proposed by Mazuran et al. (2013b, The VLDB Journal 22, 4, 471–493) and named, respectively, mcount and msum. Since mcount, and also msum on positive numbers, are monotonic in the lattice of set-containment, they preserve the fixpoint semantics of Horn Clauses. However, in many applications of practical interest, their use can lead to inefficiencies, that can be eliminated by combining them with max, whereby mcount and msum become the standard count and sum. Therefore, the semantics and optimization techniques of Datalog are extended to recursive programs with min, max, count and sum, making possible the advanced applications of superior performance and scalability demonstrated by BigDatalog (Shkapsky et al. 2016. In SIGMOD. ACM, 1135–1149) and Datalog-MC (Yang et al. 2017. The VLDB Journal 26, 2, 229–248).
“I Never Planned Anything in My Life”: The Music ofCool Hand Luke
Authors: DOERING JAMES M.
Published: August 2017

Cool Hand Lukehas a distinct soundtrack that features original music by composer Lalo Schifrin and an intriguing collection of traditional American music selected by director Stuart Rosenberg. The music emerged over an intense nine-month span in 1966–67, during which ideas flowed freely and original plans were often jettisoned. Rosenberg and Schifrin were the film's primary musical architects, but others contributed along the way, including screenwriters, actors, producers, folk music experts, and a trio of banjo players. Based on a wide range of primary sources, including documents in the Warner Bros. Archives at the University of Southern California, interviews with individuals involved in the production, the voluminous popular press about the film, and the film itself, this article is a rare glimpse inside the creative process that produced an unmistakably American soundtrack.
Ballad for Incarcerated Americans: Second Generation Japanese American Musicking in World War II Camps
Authors: ROBERTSON MARTA
Published: August 2017

During World War II, the United States government imprisoned approximately 120,000 Japanese Americans, two-thirds of whom were American-born citizens, half of whom were children. Through ethnographic interviews I explore how fragile youthful memories, trauma, and the soundscape of the War Relocation Authority (WRA) Incarceration Camps shaped the artistic trajectories of three such former “enemy alien” youth: two pianists and a koto player. Counterintuitively, Japanese traditional arts flourished in the hostile environment of dislocation through the high number ofnisei(second generation) participants, who later contributed to increasing transculturalism in American music following resettlement out of camp. Synthesizing Japanese and Euro-American classical music, white American popular music, and African American jazz, manyniseiparadoxically asserted their dual cultural commitment to both traditional Japanese and home front patriotic American principles. A performance of Earl Robinson and John Latouche's patriotic cantata,Ballad for Americans(1939), by the high school choir at Manzanar Incarceration Camp demonstrates the hybridity of these Japanese American cultural practices. Marked by Popular Front ideals,Ballad for Americansallowedniseito construct identities through a complicated mixture of ethnic pride, chauvinistic white Americanism allied with Bing Crosby's recordings of theBallad, and affiliation with black racial struggle through Paul Robeson's iconicBalladperformances.
Kurt Weill'sThe River Is Blue: “Film-Opera” and Politics in 1930s Hollywood
Authors: GRABER NAOMI
Published: August 2017

In early 1937, Kurt Weill and playwright Clifford Odets collaborated on a Spanish Civil War film that they hoped would lead to a new kind of political–musical picture. Originally titledThe River Is Blue, the film was eventually released in 1938 asBlockadewithout the involvement of either Odets or Weill, but recently uncovered archival material of the abandoned project reveals new facets of Weill's musico-dramatic thought. The discovery of Odets's first draft of the screenplay shows an extraordinarily close collaboration between composer and screenwriter as the shot numbers in the script align exactly with numbers in Weill's score. Read together, these two documents show Weill attempting to combine different paradigms of film scoring that were popular in the 1930s. There are moments of typical Golden Age–style scoring with mickey-mousing and leitmotifs, but there is also evidence of older practices such as the use of popular songs. Weill's score also displays the influence of the Popular Front, elements of Eisensteinian montage, and anti-Wagnerianism. These show Weill working toward what he termed “film-opera,” not a filmed version of an opera but an entirely new genre that combined elements of musical theatre and movies.
Probing the crystallographic orientation of two-dimensional atomic crystals with supramolecular self-assembly
Authors: Wang Jinghui , Yu Hongde , Zhou Xu , Liu Xiaozhi , Zhang Renjie , Lu Zhixing , Zheng Jingying , Gu Lin , Liu Kaihui , Wang Dong , Jiao Liying
Published: August 2017

Probing the crystallographic orientation of two-dimensional (2D) materials is essential to understand and engineer their properties. However, the nondestructive identification of the lattice orientations of various 2D materials remains a challenge due to their very thin nature. Here, we identify the crystallographic structures of various 2D atomic crystals using molecules as probes by utilizing orientation-dependent molecule–substrate interactions. We discover that the periodic atomic packing of 2D materials guides oleamide molecules to assemble into quasi-one-dimensional nanoribbons with specific alignments which precisely indicate the lattice orientations of the underlying materials. Using oleamide molecules as probes, we successfully identify the crystallographic orientations of ~12 different 2D materials without degrading their intrinsic properties. Our findings allow for the nondestructive identification of the lattice structure of various 2D atomic crystals and shed light on the functionalization of these 2D materials with supramolecular assembly.
VEGF amplifies transcription through ETS1 acetylation to enable angiogenesis
Authors: Chen Jiahuan , Fu Yi , Day Daniel S. , Sun Ye , Wang Shiyan , Liang Xiaodong , Gu Fei , Zhang Fang , Stevens Sean M. , Zhou Pingzhu , Li Kai , Zhang Yan , Lin Ruei-zeng , Smith Lois E. H. , Zhang Jin , Sun Kun , Melero-Martin Juan M. , Han Zeguang , Park Peter J. , Zhang Bing , Pu William T.
Published: August 2017

Release of promoter-proximally paused RNA polymerase II (RNAPII) is a recently recognized transcriptional regulatory checkpoint. The biological roles of RNAPII pause release and the mechanisms by which extracellular signals control it are incompletely understood. Here we show that VEGF stimulates RNAPII pause release by stimulating acetylation of ETS1, a master endothelial cell transcriptional regulator. In endothelial cells, ETS1 binds transcribed gene promoters and stimulates their expression by broadly increasing RNAPII pause release.
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VEGF enhances ETS1 chromatin occupancy and increases ETS1 acetylation, enhancing its binding to BRD4, which recruits the pause release machinery and increases RNAPII pause release. Endothelial cell angiogenic responses in vitro and in vivo require ETS1-mediated transduction of VEGF signaling to release paused RNAPII. Our results define an angiogenic pathway in which VEGF enhances ETS1–BRD4 interaction to broadly promote RNAPII pause release and drive angiogenesis.
Asymmetric dynamics of DNA entering and exiting a strongly confining nanopore
Authors: Bell Nicholas A. W. , Chen Kaikai , Ghosal Sandip , Ricci Maria , Keyser Ulrich F.
Published: August 2017

In nanopore sensing, changes in ionic current are used to analyse single molecules in solution. The translocation dynamics of polyelectrolytes is of particular interest given potential applications such as DNA sequencing. In this paper, we determine how the dynamics of voltage driven DNA translocation can be affected by the nanopore geometry and hence the available configurational space for the DNA. Using the inherent geometrical asymmetry of a conically shaped nanopore, we examine how DNA dynamics depends on the directionality of transport. The total translocation time of DNA when exiting the extended conical confinement is significantly larger compared to the configuration where the DNA enters the pore from the open reservoir. By using specially designed DNA molecules with positional markers, we demonstrate that the translocation velocity progressively increases as the DNA exits from confinement. We show that a hydrodynamic model can account for these observations.
Using ALoFT to determine the impact of putative loss-of-function variants in protein-coding genes
Authors: Balasubramanian Suganthi , Fu Yao , Pawashe Mayur , McGillivray Patrick , Jin Mike , Liu Jeremy , Karczewski Konrad J. , MacArthur Daniel G. , Gerstein Mark
Published: August 2017

Variants predicted to result in the loss of function of human genes have attracted interest because of their clinical impact and surprising prevalence in healthy individuals. Here, we present ALoFT (annotation of loss-of-function transcripts), a method to annotate and predict the disease-causing potential of loss-of-function variants. Using data from Mendelian disease-gene discovery projects, we show that ALoFT can distinguish between loss-of-function variants that are deleterious as heterozygotes and those causing disease only in the homozygous state. Investigation of variants discovered in healthy populations suggests that each individual carries at least two heterozygous premature stop alleles that could potentially lead to disease if present as homozygotes. When applied to de novo putative loss-of-function variants in autism-affected families, ALoFT distinguishes between deleterious variants in patients and benign variants in unaffected siblings. Finally, analysis of somatic variants in >6500 cancer exomes shows that putative loss-of-function variants predicted to be deleterious by ALoFT are enriched in known driver genes.
Improved split fluorescent proteins for endogenous protein labeling
Authors: Feng Siyu , Sekine Sayaka , Pessino Veronica , Li Han , Leonetti Manuel D. , Huang Bo
Published: August 2017

Self-complementing split fluorescent proteins (FPs) have been widely used for protein labeling, visualization of subcellular protein localization, and detection of cell–cell contact. To expand this toolset, we have developed a screening strategy for the direct engineering of self-complementing split FPs. Via this strategy, we have generated a yellow–green split-mNeonGreen21–10/11 that improves the ratio of complemented signal to the background of FP1–10-expressing cells compared to the commonly used split GFP1–10/11; as well as a 10-fold brighter red-colored split-sfCherry21–10/11. Based on split sfCherry2, we have engineered a photoactivatable variant that enables single-molecule localization-based super-resolution microscopy. We have demonstrated dual-color endogenous protein tagging with sfCherry211 and GFP11, revealing that endoplasmic reticulum translocon complex Sec61B has reduced abundance in certain peripheral tubules. These new split FPs not only offer multiple colors for imaging interaction networks of endogenous proteins, but also hold the potential to provide orthogonal handles for biochemical isolation of native protein complexes.
An allosteric ligand-binding site in the extracellular cap of K2P channels
Authors: Luo Qichao , Chen Liping , Cheng Xi , Ma Yuqin , Li Xiaona , Zhang Bing , Li Li , Zhang Shilei , Guo Fei , Li Yang , Yang Huaiyu
Published: August 2017

Two-pore domain potassium (K2P) channels generate leak currents that are responsible for the maintenance of the resting membrane potential, and they are thus potential drug targets for treating diseases. Here, we identify N-(4-cholorphenyl)-N-(2-(3,4-dihydrosioquinolin-2(1H)-yl)-2-oxoethyl)methanesulfonamide (TKDC) as an inhibitor of the TREK subfamily, including TREK-1, TREK-2 and TRAAK channels. Using TKDC as a chemical probe, a study combining computations, mutagenesis and electrophysiology reveals a K2P allosteric ligand-binding site located in the extracellular cap of the channels. Molecular dynamics simulations suggest that ligand-induced allosteric conformational transitions lead to blockage of the ion conductive pathway. Using virtual screening approach, we identify other inhibitors targeting the extracellular allosteric ligand-binding site of these channels. Overall, our results suggest that the allosteric site at the extracellular cap of the K2P channels might be a promising drug target for these membrane proteins.
Differential dynamic microscopy of bidisperse colloidal suspensions
Authors: Safari Mohammad S. , Poling-Skutvik Ryan , Vekilov Peter G. , Conrad Jacinta C.
Published: August 2017

Research tasks in microgravity include monitoring the dynamics of constituents of varying size and mobility in processes such as aggregation, phase separation, or self-assembly. We use differential dynamic microscopy, a method readily implemented with equipment available on the International Space Station, to simultaneously resolve the dynamics of particles of radius 50 nm and 1 μm in bidisperse aqueous suspensions. Whereas traditional dynamic light scattering fails to detect a signal from the larger particles at low concentrations, differential dynamic microscopy exhibits enhanced sensitivity in these conditions by accessing smaller wavevectors where scattering from the large particles is stronger. Interference patterns due to scattering from the large particles induce non-monotonic decay of the amplitude of the dynamic correlation function with the wavevector. We show that the position of the resulting minimum contains information on the vertical position of the particles. Together with the simple instrumental requirements, the enhanced sensitivity of differential dynamic microscopy makes it an appealing alternative to dynamic light scattering to characterize samples with complex dynamics.
Model-Based Therapy Planning Allows Prediction of Haemodynamic Outcome after Aortic Valve Replacement
Authors: Kelm M. , Goubergrits L. , Bruening J. , Yevtushenko P. , Fernandes J. F. , Sündermann S. H. , Berger F. , Falk V. , Kuehne T. , Nordmeyer S. ,   CARDIOPROOF group, Morley-Fletcher E. , De Maldè M. , Muthurangu V. , Khushnood A. , Chinali M. , Pongiglione G. , Hennemuth A. , Mirzae H. , Neugebauer M. , Ecabert O. , Neumann D. , Groenenboom P. , Plank G. , Manset D. , McGuire A. , Naci H. , Salcher M.
Published: August 2017

Optimizing treatment planning is essential for advances in patient care and outcomes. Precisely tailored therapy for each patient remains a yearned-for goal. Cardiovascular modelling has the potential to simulate and predict the functional response before the actual intervention is performed. The objective of this study was to proof the validity of model-based prediction of haemodynamic outcome after aortic valve replacement. In a prospective study design virtual (model-based) treatment of the valve and the surrounding vasculature were performed alongside the actual surgical procedure (control group). The resulting predictions of anatomic and haemodynamic outcome based on information from magnetic resonance imaging before the procedure were compared to post-operative imaging assessment of the surgical control group in ten patients. Predicted vs. post-operative peak velocities across the valve were comparable (2.97 ± 1.12 vs. 2.68 ± 0.67 m/s; p = 0.362). In wall shear stress (17.3 ± 12.3 Pa vs. 16.7 ± 16.84 Pa; p = 0.803) and secondary flow degree (0.44 ± 0.32 vs. 0.49 ± 0.23; p = 0.277) significant linear correlations (p < 0.001) were found between predicted and post-operative outcomes. Between groups blood flow patterns showed good agreement (helicity p = 0.852, vorticity p = 0.185, eccentricity p = 0.333). Model-based therapy planning is able to accurately predict post-operative haemodynamics after aortic valve replacement. These validated virtual treatment procedures open up promising opportunities for individually targeted interventions.
Regulation of Orai1/STIM1 mediated ICRAC by intracellular pH
Authors: Gavriliouk D. , Scrimgeour N. R. , Grigoryev S. , Ma L. , Zhou F. H. , Barritt G. J. , Rychkov G. Y.
Published: August 2017

Ca2+ release activated Ca2+ (CRAC) channels composed of two cellular proteins, Ca2+-sensing stromal interaction molecule 1 (STIM1) and pore-forming Orai1, are the main mediators of the Ca2+ entry pathway activated in response to depletion of intracellular Ca2+ stores. Previously it has been shown that the amplitude of CRAC current (ICRAC) strongly depends on extracellular and intracellular pH. Here we investigate the intracellular pH (pHi) dependence of ICRAC mediated by Orai1 and STIM1ectopically expressed in HEK293 cells. The results indicate that pHi affects not only the amplitude of the current, but also Ca2+ dependent gating of CRAC channels. Intracellular acidification changes the kinetics of ICRAC, introducing prominent re-activation component in the currents recorded in response to voltage steps to strongly negative potentials. ICRAC with similar kinetics can be observed at normal pHi if the expression levels of Orai1 are increased, relative to the expression levels of STIM1. Mutations in the STIM1 inactivation domain significantly diminish the dependence of ICRAC kinetics on pHi, but have no effect on pHi dependence of ICRAC amplitude, implying that more than one mechanism is involved in CRAC channel regulation by intracellular pH.
2 + 1 dimensional de Sitter universe emerging from the gauge structure of a nonlinear quantum system
Authors: Kam Chon-Fai , Liu Ren-Bao
Published: August 2017

Berry phases and gauge structures are fundamental quantum phenomena. In linear quantum mechanics the gauge field in parameter space presents monopole singularities where the energy levels become degenerate. In nonlinear quantum mechanics, which is an effective theory of interacting quantum systems, there can be phase transitions and hence critical surfaces in the parameter space. We find that these critical surfaces result in a new type of gauge field singularity, namely, a conic singularity that resembles the big bang of a 2 + 1 dimensional de Sitter universe, with the fundamental frequency of Bogoliubov excitations acting as the cosmic scale, and mode softening at the critical surface, where the fundamental frequency vanishes, causing a causal singularity. Such conic singularity may be observed in various systems such as Bose-Einstein condensates and molecular magnets. This finding offers a new approach to quantum simulation of fundamental physics.
Cu-chitosan nanoparticle boost defense responses and plant growth in maize (Zea mays L.)
Authors: Choudhary Ram Chandra , Kumaraswamy R. V. , Kumari Sarita , Sharma S. S. , Pal Ajay , Raliya Ramesh , Biswas Pratim , Saharan Vinod
Published: August 2017

In agriculture, search for biopolymer derived materials are in high demand to replace the synthetic agrochemicals. In the present investigation, the efficacy of Cu-chitosan nanoparticles (NPs) to boost defense responses against Curvularia leaf spot (CLS) disease of maize and plant growth promotry activity were evaluated. Cu-chitosan NPs treated plants showed significant defense response through higher activities of antioxidant (superoxide dismutase and peroxidase) and defense enzymes (polyphenol oxidase and phenylalanine ammonia-lyase). Significant control of CLS disease of maize was recorded at 0.04 to 0.16% of Cu-chitosan NPs treatments in pot and 0.12 to 0.16% of NPs treatments in field condition. Further, NPs treatments exhibited growth promotry effect in terms of plant height, stem diameter, root length, root number and chlorophyll content in pot experiments. In field experiment, plant height, ear length, ear weight/plot, grain yield/plot and 100 grain weight were enhanced in NPs treatments. Disease control and enhancement of plant growth was further enlightened through Cu release profile of Cu-chitosan NPs. This is an important development in agriculture nanomaterial research where biodegradable Cu-chitosan NPs are better compatible with biological control as NPs “mimic” the natural elicitation of the plant defense and antioxidant system for disease protection and sustainable growth.