bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Boundary Conditions on the Performance Enhancement of Advanced Control Strategies for a Residential Building with a Heat Pump and PV System with Energy Storage
Authors: Psimopoulos Emmanouil , Johari Fatemeh , Bales Chris , Widén Joakim
Published: March 2020

Operational control strategies for the heating system of a single-family house with exhaust air heat pump and photovoltaic system and “smart” utilization of energy storage have been developed and evaluated in a simulation study. The main aim and novelty of this study is to evaluate the impact on the benefit of these advanced control strategies in terms of performance (energy use and economic) for a wide range of boundary conditions (country/climate, occupancy and appliance loads). Short-term weather data and historic price data for the same year as well as stochastic occupancy profiles that include the domestic hot water load are used as boundary for a parametric simulation study for the system modeled in detail in TRNSYS 17. Results show that the control using a forecast of dynamic electricity price leads to greater final energy savings than those due to the control using thermal storage for excess PV production in all of the examined locations except Sweden. The impact on self-consumption using thermal storage of heat produced by the heat pump using excess PV production is found to decrease linearly with increasing household electricity for all locations. A reduction in final energy of up to 842 kWh year−1 can be achieved just by the use of these algorithms. The net energy cost for the end-user follows the same trend as for final energy and can result in cost savings up to 175 € year−1 in Germany and Spain due to the use of the advanced control.
Urban Warming and Cities’ Microclimates: Investigation Methods and Mitigation Strategies—A Review
Authors: Tsoka Stella , Tsikaloudaki Katerina , Theodosiou Theodoros , Bikas Dimitrios
Published: March 2020

The increased rates of urbanization and industrialization of the 20th and 21st centuries have dramatically changed the land use and cover of modern cities, contributing to the degradation of the urban microclimate and the rise of the ambient urban air temperatures. Given the multiple negative energy, environmental and social consequences of urban warming, the present paper summarizes the findings of previous studies, assessing the main causes of the phenomenon along with the key investigation methods involving experimental and computational approaches. There follows a description of the most common mitigations, and adaption strategies towards the attenuation of urban warming are described. The analyzed elements include the addition of green spaces such as trees, grass and green roofs; changes on the albedo of the urban surfaces and water-based techniques, as well as a combination of them. The discussion of the reported findings in the existing literature clearly reflects the impact of urban morphology on the outdoor thermal environment, providing also useful information for professionals and urban planners involved at the phase of decision-making.
The Integrated Energy Consumption Index for Energy Biomass Grinding Technology Assessment
Authors: Kruszelnicka Weronika , Kasner Robert , Bałdowska-Witos Patrycja , Flizikowski Józef , Tomporowski Andrzej
Published: March 2020

The assessment of engineering objects in terms of energy consumption is an important part of sustainable development. Many materials, including those from the energy sector, need to undergo earlier processing, e.g., grinding. Grinding processes still demand a significant amount of energy, whereas current energy assessment methods do not take into account important parameters of the process, which makes it difficult to choose their optimal values. The study presents the analysis, testing, and assessment of mechanical engineering systems in terms of the energy consumption involved in the grinding of biomass intended for energy production purposes. A testing methodology was developed to improve the parameters of multi-disc grinding, including the reduction of energy consumption, power input, product quality improvement, and process efficiency. An original model of integrated energy consumption was developed. Tests were carried out on a five-disc grinder for five programs to assess the programmable angular speeds of the grinder discs. Output values, including specific energy demand, fragmentation degree, and integrated energy consumption, were assigned to each testing program. The test results were subjected to statistical analysis. Based on the authors’ own research, it was found that the angular speed of the discs and, consequently, the linear speed of the grinding blades, have a significant influence on the values of the integrated energy consumption of the preliminary process.
Efficiency Optimization for All-Silicon Carbide (SiC) PWM Rectifier Considering the Impact of Gate-Source Voltage Interference
Authors: Li Zhijun , Wang Zuoxing , Zheng Trillion , Li Hong , Huang Bo , Shao Tiancong
Published: March 2020

Compared with conventional silicon (Si)-based Pulse Width Modulation (PWM) rectifiers, PWM rectifiers based on silicon carbide (SiC) Metal-Oxide-Semiconductor Field-Effect Transistors (MOSFETs) have significant technical advantages and broad application prospects in terms of efficiency and power density, inherited from the high-speed switching feature. However, high-speed switching also induces gate-source voltage interference, which impacts the overall character of the conversion system. This paper considered the impact of gate-source voltage interference on loss, revealing an efficiency optimization for all-SiC PWM rectifiers. Firstly, this paper theoretically investigated the mechanism of improving the conversion system efficiency by using the 4-pin Kelvin packaged SiC MOSFETs. Then, based on the industrial product case study, loss distribution, using different package styles, was quantitatively analyzed. Finally, experiment test results verified the efficiency improvement of the PWM rectifier with the 4-pin Kelvin package SiC MOSFETs.
A Conceptual Framework to Classify and Manage Risk, Uncertainty and Ambiguity: An Application to Energy Policy
Authors: Gaudard Ludovic , Romerio Franco
Published: March 2020

Global issues are such that we should assess and manage a variety of risks and uncertainties. Due to increasing world complexity, the development of an adequate and innovative conceptual framework, anchored in the literature, is required. This article contributes to this effort with an approach particularly relevant to decision-makers dealing with threats of different natures, limited heterogeneous information, and experts’ assessments tainted by doubts. Our approach is based on two pillars: 1) An “acuity scale”, based on the probability of the occurrence of an event, its impact and the experts’ degree of doubt; 2) A taxonomy focused on the concepts of risk, uncertainty, gamble and butterfly ambiguity. Accordingly, we present in a second step the major management implications of such approach. Global policy trends (e.g., sustainability transition) put energy sector decision-makers at the forefront of risk and uncertainty management. Consequently, we carry out a case study focused on Swiss energy policy since the 1980s, including its inception, the turnaround provoked by the Fukushima accident, and the government’s 2050 energy strategy. Our investigation shows that the proposed conceptual framework allows for the development of an original analysis of the main drivers that influence governmental policies and stakeholder strategies.
Decision-Making Process for Photovoltaic Solar Energy Sector Development using Fuzzy Cognitive Map Technique
Authors: Papageorgiou Konstantinos , Carvalho Gustavo , Papageorgiou Elpiniki I. , Bochtis Dionysis , Stamoulis George
Published: March 2020

Photovoltaic Solar Energy (PSE) sector has sparked great interest from governments over the last decade towards diminution of world’s dependency to fossil fuels, greenhouse gas emissions reduction and global warming mitigation. Photovoltaic solar energy also holds a significant role in the transition to sustainable energy systems. These systems and their optimal exploitation require an effective supply chain management system, such as design of the network, collection, storage, or transportation of this energy resource, without disregarding a country’s certain socio-economic and political conditions. In Brazil, the adoption of photovoltaic solar energy has been motivated not only by the energy matrix diversification but also from the shortages, problems, and barriers that the Brazilian energy sector has faced, lately. However, PSE development is affected by various factors with high uncertainty, such as political, social, economic, and environmental, that include critical operational sustainability issues. Thus, an elaborate modelling of energy management and a well-structured decision support process are needed to enhance the performance efficiency of Brazilian PSE supply chain management. This study focuses on the investigation of certain factors and their influence on the development of the Brazilian PSE with the help of Fuzzy Cognitive Maps. Fuzzy Cognitive Map is an established methodology for scenario analysis and management in diverse domains, inheriting the advancements of fuzzy logic and neural networks. In this context, a semi-quantitative model was designed with the help of various stakeholders from the specific energy domain and three plausible scenarios were conducted in order to support a decision-making process on PSE sector development and the country’s economic potential. The outcome of this analysis reveals that the development of the PSE sector in Brazil is mainly affected by economic and political factors.
Climate Adaptive Design Improvement Strategies of Traditional Dwellings in Southern Zhejiang for the Plum Rain Season Considering Comfort Conditions
Authors: Zheng Ruifeng , Zheng Yufeng , Cong Lei , Choi Joon-Ho , Jung Hyun
Published: March 2020

This study investigated the adaptations of traditional dwellings to the complex regional microclimate in southern Zhejiang, China. Typical traditional dwellings in a village in the foothills and a village on the mid-slopes of Zhejiang’s alpine region were selected to study traditional construction strategies for climate responsiveness and the comfort level of indoor environments during the very humid plum rain season in early summer. Fundamental analysis of the climate and architecture, a response analysis of the dwelling form, an occupants’ comfort satisfaction survey, and field measurements of indoor and outdoor thermal environmental parameters were performed. The traditional dwellings and their design strategies for various regional environmental factors were explored from the perspective of (1) regional climate-adaptive strategies, (2) the thermal, airflow, lighting, and acoustic qualities of the indoor environment, and (3) the occupants’ indoor environment satisfaction. The results indicated that traditional dwellings in southern Zhejiang incorporate strategies of various effectiveness in ensuring indoor comfort.
Three-Dimensional Fibrous Iron as Anode Current Collector for Rechargeable Zinc–Air Batteries
Authors: Khezri Ramin , Jirasattayaporn Kridsada , Abbasi Ali , Maiyalagan Thandavarayan , Mohamad Ahmad Azmin , Kheawhom Soorathep
Published: March 2020

A three-dimensional (3D) fibrous structure with a high active surface and conductive pathway proved to be an excellent anode current collector for rechargeable zinc–air batteries (ZABs). Herein, a cost-effective and highly stable zinc (Zn) electrode, based on Zn electrodeposited on iron fibers (Zn/IF), is duly examined. Electrochemical characteristics of the proposed electrode are seen to compete with a conventional zinc/nickel foam (Zn/NF) electrode, implying that it can be a suitable alternative for use in ZABs. Results show that the Zn/IF electrode exhibits an almost similar trend as Zn/NF in cyclic voltammetry (CV). Moreover, by using a Zn/IF electrode, electrochemical impedance spectroscopy (EIS) demonstrates lower charge transfer resistance. In the application of a rechargeable ZAB, the fibrous Zn/IF electrode exhibits a high coulombic efficiency (CE) of 78%, close to the conventional Zn/NF (80%), with almost similar capacity and lower charge transfer resistance, after 200 charge/discharge cycles. It is evident that all the positive features of Zn/IF, especially its low cost, shows that it can be a valuable anode for ZABs.
Lactic Acid Bacteria: Variability Due to Different Pork Breeds, Breeding Systems and Fermented Sausage Production Technology
Authors: Comi Giuseppe , Muzzin Alessia , Corazzin Mirco , Iacumin Lucilla
Published: March 2020

Changes in the ecology of the various lactic acid bacteria (LAB) species, which are involved in traditional fermented sausages, were investigated in the light of the use of different breeds of pork, each of which was raised in two different environments and processed using two different technologies. The semi-quantitative molecular method was applied in order to understand how the different species alternate over time, as well as their concentration ratios. A significant increase in LAB over the first days of fermentation characterized the trials where the starter culture wasn’t added (T), reaching values of 107–108 cfu g−1. On the other hand, in the trials in which sausages were produced with starter addition, LAB counts had a less significant incremental jump from about 106 cfu g−1 (concentration of the inoculum) to 108 cfu g−1. Lactobacillus sakei and Lb. curvatus were detected as the prevalent population in all the observed fermentations. Pediococcus pentosaceus, Lb. casei, Leuconostoc mesenteroides, Lactococcus garviae, and Lb. graminis also appeared, but their concentration ratios varied depending on the diverse experimental settings. The results of cluster analysis showed that a plant- and breed-specific LAB ecology exists. In addition, it was also observed that the breeding system can influence the presence of certain LAB species.
Sensory Profile and Physico-Chemical Properties of Artisanal Honey from Zulia, Venezuela
Authors: Araujo Deyanira , Ruiz Pérez-Cacho Pilar , Serrano Salud , Dios-Palomares Rafaela , Galán-Soldevilla Hortensia
Published: March 2020

The physico-chemical parameters and the sensory profile were determined in honeys from apiaries of the Mara and Maracaibo of Zulia State (Venezuela). The analysis of variance showed that there were no significant differences in the mean value between apiaries for most of the physicochemical parameters and sensory attributes. The obtained value for pH (3.58–4.08), free acidity (30.9–36.0 meq/kg), lactone acidity (9.0–14.3 meq/kg), total acidity (42.1–46.0 meq/kg), moisture content (19.1–20.0%), diastase activity (8.11–12.7 ºG), colour intensity (41.5–86.6 mm Pfund), hydroxymethylfurfural (15.7–26.0 mg/kg), and electrical conductivity (0.33–0.52 mS/cm) were within the criteria set by international quality regulations. The sensory profile of these honeys is characterized by being amber in colour, with a floral, acid fruit, balsamic and animal odour/aroma, a sweet, slightly acid taste, and by being fluid and of a medium persistence.
Health Benefits and Molecular Mechanisms of Resveratrol: A Narrative Review
Authors: Meng Xiao , Zhou Jing , Zhao Cai-Ning , Gan Ren-You , Li Hua-Bin
Published: March 2020

Resveratrol is a bioactive compound in many foods. Since its anticancer activity was reported in 1997, its health benefits have been intensively investigated. Resveratrol has antioxidant, anti-inflammatory, immunomodulatory, glucose and lipid regulatory, neuroprotective, and cardiovascular protective effects, therefore, can protect against diverse chronic diseases, such as cardiovascular diseases (CVDs), cancer, liver diseases, obesity, diabetes, Alzheimer’s disease, and Parkinson’s disease. This review summarizes the main findings of resveratrol-related health benefits in recent epidemiological surveys, experimental studies, and clinical trials, highlighting its related molecular mechanisms. Resveratrol, therefore, has been regarded as a potent candidate for the development of nutraceuticals and pharmaceuticals to prevent and treat certain chronic diseases.
Fast Quantification of Honey Adulteration with Laser-Induced Breakdown Spectroscopy and Chemometric Methods
Authors: Peng Jiyu , Xie Weiyue , Jiang Jiandong , Zhao Zhangfeng , Zhou Fei , Liu Fei
Published: March 2020

Honey adulteration is a major issue in food production, which may reduce the effective components in honey and have a detrimental effect on human health. Herein, laser-induced breakdown spectroscopy (LIBS) combined with chemometric methods was used to fast quantify the adulterant content. Two common types of adulteration, including mixing acacia honey with high fructose corn syrup (HFCS) and rape honey, were quantified with univariate analysis and partial least squares regression (PLSR). In addition, the variable importance was tested with univariable analysis and feature selection methods (genetic algorithm (GA), variable importance in projection (VIP), selectivity ratio (SR)). The results indicated that emissions from Mg II 279.58, 280.30 nm, Mg I 285.25 nm, Ca II 393.37, 396.89 nm, Ca I 422.70 nm, Na I 589.03, 589.64 nm, and K I 766.57, 769.97 nm had compact relationship with adulterant content. Best models for detecting the adulteration ratio of HFCS 55, HFCS 90, and rape honey were achieved by SR-PLSR, VIP-PLSR, and VIP-PLSR, with root-mean-square error (RMSE) of 8.9%, 8.2%, and 4.8%, respectively. This study provided a fast and simple approach for detecting honey adulteration.
Effect of Breed and Diet Type on the Freshness and Quality of the Eggs: A Comparison between Mos (Indigenous Galician Breed) and Isa Brown Hens
Authors: Franco Daniel , Rois Diego , Arias Anisia , Justo José Ramón , Marti-Quijal Francisco J. , Khubber Sucheta , Barba Francisco J. , López-Pedrouso María , Manuel Lorenzo José
Published: March 2020

Eggs are a nutritious food, offering a balanced source of essential amino and fatty acids, minerals, and vitamins. Genetic and diet factors can modify hen egg traits. Thus, the effects of breed and feed on egg quality using two laying hens, Mos (autochthonous breed) and Isa Brown (commercial hybrid), and three feeds, commercial fodder (CF), corn/pea/triticale (CPT) and corn/wheat (CW), were investigated. Freshness parameters (egg weight, eggshell weight and thickness, albumen height, Haugh units and yolk color), chemical composition, color and textural parameters, as well as fatty acid profile, were assessed on a total of 288 eggs, from the two breeds. The samples were divided in 96 eggs, corresponding to each of the three dietary treatments. There were significant differences (p < 0.001) in albumen height and Haugh units, obtaining the highest values for Isa Brown genotype; meanwhile, laying hens fed with CF had the highest weight, as well as the greatest eggshell thickness. Cooked yolks of Isa Brown eggs presented the highest values of luminosity, while the yellowness was higher for Mos eggs. Regarding the texture of eggs, genotype was again the parameter having the greatest impact, obtaining higher values in hardness, gumminess and chewiness in those eggs from the Mos breed. Concerning egg chemical composition, it was affected by breed and diet type, but Mos eggs were characterized by a significantly (p < 0.001) higher contents of fat (9.53% vs. 7.58%), protein (12.31% vs. 11.66%) and ash (1.10% vs. 1.04%) than Isa Brown ones. Finally, diet type influenced the fatty acid profile, mainly affecting oleic and linoleic acids, which showed significantly (p < 0.05) highest values (42.90 and 14.66 g/100 g of total fatty acids) in CW and CF diets, respectively. Overall, breed and bird diet factors had a strong effect on egg quality and nutritional profile. Moreover, eggs from Mos hens had more attractive nutritional indices, and they could even be improved more by changing the diet.
Toxicity and Synergistic Effect of Elsholtzia ciliata Essential Oil and Its Main Components against the Adult and Larval Stages of Tribolium castaneum
Authors: Liang Jun-Yu , Xu Jie , Yang Ying-Ying , Shao Ya-Zhou , Zhou Feng , Wang Jun-Long
Published: March 2020

Investigations have indicated that storage pests pose a great threat to global food security by damaging food crops and other food products derived from plants. Essential oils are proven to have significant effects on a large number of stored grain insects. This study evaluated the contact toxicity and fumigant activity of the essential oil extract from the aerial parts of Elsholtzia ciliata and its two major biochemical components against adults and larvae of the food storage pest beetle Tribolium castaneum. Gas chromatography–mass spectrometry analysis revealed 16 different components derived from the essential oil of E. ciliata, which included carvone (31.63%), limonene (22.05%), and α-caryophyllene (15.47%). Contact toxicity assay showed that the essential oil extract exhibited a microgram-level of killing activity against T. castaneum adults (lethal dose 50 (LD50) = 7.79 μg/adult) and larvae (LD50 = 24.87 μg/larva). Fumigant toxicity assay showed LD50 of 11.61 mg/L air for adults and 8.73 mg/L air for larvae. Carvone and limonene also exhibited various levels of bioactivity. A binary mixture (2:6) of carvone and limonene displayed obvious contact toxicity against T. castaneum adults (LD50 = 10.84 μg/adult) and larvae (LD50 = 30.62 μg/larva). Furthermore, carvone and limonene exhibited synergistic fumigant activity against T. castaneum larvae at a 1:7 ratio. Altogether, our results suggest that E. ciliata essential oil and its two monomers have a potential application value to eliminate T. castaneum.
Characterization of Volatile Profiles and Marker Substances by HS-SPME/GC-MS during the Concentration of Coconut Jam
Authors: Zhang Hao , Chen Haiming , Wang Wenzhu , Jiao Wenxiao , Chen Wenxue , Zhong Qiuping , Yun Yong-Huan , Chen Weijun
Published: March 2020

Characteristic aromas are usually key labels for food products. In this study, the volatile profiles and marker substances of coconut jam during concentration were characterized via sensory evaluation combined with headspace solid phase microextraction-gas chromatography-tandem mass spectrometry (HSPME/GC-MS). A total of 33 aroma compounds were detected by HSPME/GC-MS. Principal component analysis revealed the concentration process of coconut jam can be divided into three stages. In the first stage, esters and alcohols were the two main contributors to the aroma of the coconut jam. Next, a caramel smell was gradually formed during the second stage, which was mainly derived from aldehydes, ketones and alcohols. The concentration of aldehydes increased gradually at this stage, which may be the result of a combination of the Maillard reaction and the caramelization reaction. In the final sterilization stage, the ‘odor intensity’ of caramel reached the maximum level and a variety of aroma compounds were produced, thereby forming a unique flavor for the coconut jam. Finally, furfural fit a logistic model with a regression coefficient (r2) of 0.97034. Therefore, furfural can be used as a marker substance for monitoring the concentration of coconut jam.
Bifidobacterium longum subsp. infantis ATCC 15697 and Goat Milk Oligosaccharides Show Synergism In Vitro as Anti-Infectives against Campylobacter jejuni
Authors: Quinn Erinn M. , Slattery Helen , Walsh Dan , Joshi Lokesh , Hickey Rita M.
Published: March 2020

Bifidobacteria are known to inhibit, compete with and displace the adhesion of pathogens to human intestinal cells. Previously, we demonstrated that goat milk oligosaccharides (GMO) increased the attachment of Bifidobacterium longum subsp. infantis ATCC 15697 to intestinal cells in vitro. In this study, we aimed to exploit this effect as a mechanism for inhibiting pathogen association with intestinal cells. We examined the synergistic effect of GMO-treated B. infantis on preventing the attachment of a highly invasive strain of Campylobacter jejuni to intestinal HT-29 cells. The combination decreased the adherence of C. jejuni to the HT-29 cells by an average of 42% compared to the control (non-GMO treated B. infantis). Increasing the incubation time of the GMO with the Bifidobacterium strain resulted in the strain metabolizing the GMO, correlating with a subsequent 104% increase in growth over a 24 h period when compared to the control. Metabolite analysis in the 24 h period also revealed increased production of acetate, lactate, formate and ethanol by GMO-treated B. infantis. Statistically significant changes in the GMO profile were also demonstrated over the 24 h period, indicating that the strain was digesting certain structures within the pool such as lactose, lacto-N-neotetraose, lacto-N-neohexaose 3′-sialyllactose, 6′-sialyllactose, sialyllacto-N-neotetraose c and disialyllactose. It may be that early exposure to GMO modulates the adhesion of B. infantis while carbohydrate utilisation becomes more important after the bacteria have transiently colonised the host cells in adequate numbers. This study builds a strong case for the use of synbiotics that incorporate oligosaccharides sourced from goat′s milk and probiotic bifidobacteria in functional foods, particularly considering the growing popularity of formulas based on goat milk.
Arthrospira platensis as Natural Fermentation Booster for Milk and Soy Fermented Beverages
Authors: Martelli Francesco , Alinovi Marcello , Bernini Valentina , Gatti Monica , Bancalari Elena
Published: March 2020

Arthrospira platensis, commercially known as Spirulina, is a fresh-water cyanobacterium that has been gaining increasing attention in recent years due to its high biological and nutritional value. For this reason, it has been employed in several food applications, to obtain or enhance functional and technological properties of cheese, yogurt, bread, cookies or pasta. The aim of this work was to evaluate the potential boosting effect of two different concentrations (0.25% and 0.50% w/v) of A. platensis on the fermentation capability of several starter lactic acid bacteria (LAB) strains, 1 probiotic and 4 commercial mix culture. These strains were used to ferment three different substrates and their fermentation behaviors were evaluated by impedance analyses together with rheological and color measurements. In tryptic soy broth (TSB), the A. platensis boosting effect was significantly higher if compared to yeast extract for all the starter LAB strains except for Lb. casei, which was equally stimulated. Different results were found when the same LAB strains were cultivated in SSM. The most evident boosting effect was found for S. thermophilus and Lb. casei. LAB growth was promoted by A. platensis, confirming that it could be a useful tool in the production of novel functional fermented dairy foods. The potential boosting effect was evaluated on four commercial mix cultures used to produce milk and soy fermented beverages. It was demonstrated that the booster effect took place, but it was variable and dependent not only on the mix culture used, but also on the substrate and A. platensis concentration. Also, rheological and color modifications were found to be dependent on these factors.
Factors Influencing Purchase Intention for Low-Sodium and Low-Sugar Products
Authors: Park Yeowoon , Lee Dongmin , Park Seoyoung , Moon Junghoon
Published: March 2020

As sodium and sugar intake in South Korea exceed recommended levels, the government and food industry have been attempting to reduce the amount of sodium and sugar in the food products. In line with these efforts, this study sought to examine how the purchase intention for low-sodium/low-sugar products vary based on consumers’ previous choices of low-sodium/low-sugar products and other consumer-related factors. For this study, two online survey-based experiments were conducted: one using soy sauce to represent a sodium-based product and the other using yogurt to represent a sugar-based product. The significant variables that influenced the purchase intention for both were the consumers’ previous low-sodium/low-sugar product choices and their propensity for food neophobia. Consumers who had previously selected regular products showed a lower intention to purchase low-sodium soy sauce or low-sugar yogurt. In addition, those who had a strong tendency toward food neophobia also had a significantly lower purchase intention for these products. Moreover, the lower the consumer′s unhealthy = tasty intuition (UTI), the higher the purchase intention for the low-sodium soy sauce, but UTI did not act as a significant variable for the low-sugar yogurt. These results demonstrate that government interventions for low-sodium products and low-sugar products should be differentiated.
Effect of Grape Pomace Addition on the Technological, Sensory, and Nutritional Properties of Durum Wheat Pasta
Authors: Tolve Roberta , Pasini Gabriella , Vignale Fabiola , Favati Fabio , Simonato Barbara
Published: March 2020

In this study, fortified pasta was prepared by replacing semolina with 0, 5, and 10 g/100 g of grape pomace (GP), a food industry by-product, rich in fiber and phenols. GP inclusion in pasta significantly reduced its optimum cooking time and the swelling index, while also increasing the cooking loss (p < 0.05). Furthermore, pasta firmness and adhesiveness were enhanced by the GP addition, as well as the total phenol content and the antioxidant activity, evaluated through ABTS and FRAP assays (p < 0.05). From a nutritional point of view, increasing amounts of GP resulted in a significative decrease in the rapidly digestible starch and an increase in the slowly digestible starch, while the predicted in vitro glycemic index was also reduced (p < 0.05). Sensory analysis showed that fortified spaghetti had good overall acceptability, and the results suggest that GP-fortified pasta could represent a healthy product with good technological and sensory properties.
Alignment and Proficiency of Virgin Olive Oil Sensory Panels: The OLEUM Approach
Authors: Barbieri Sara , Brkić Bubola Karolina , Bendini Alessandra , Bučar-Miklavčič Milena , Lacoste Florence , Tibet Ummuhan , Winkelmann Ole , García-González Diego Luis , Gallina Toschi Tullia
Published: March 2020

A set of 334 commercial virgin olive oil (VOO) samples were evaluated by six sensory panels during the H2020 OLEUM project. Sensory data were elaborated with two main objectives: (i) to classify and characterize samples in order to use them for possible correlations with physical–chemical data and (ii) to monitor and improve the performance of panels. After revision of the IOC guidelines in 2018, this work represents the first published attempt to verify some of the recommended quality control tools to increase harmonization among panels. Specifically, a new “decision tree” scheme was developed, and some IOC quality control procedures were applied. The adoption of these tools allowed for reliable classification of 289 of 334 VOOs; for the remaining 45, misalignments between panels of first (on the category, 21 cases) or second type (on the main perceived defect, 24 cases) occurred. In these cases, a “formative reassessment” was necessary. At the end, 329 of 334 VOOs (98.5%) were classified, thus confirming the effectiveness of this approach to achieve a better proficiency. The panels showed good performance, but the need to adopt new reference materials that are stable and reproducible to improve the panel’s skills and agreement also emerged.
A Glycoside Hydrolase Family 62 A-L-Arabinofuranosidase from Trichoderma Reesei and Its Applicable Potential during Mashing
Authors: Sun Junyong , Xu Feng , Lu Jian
Published: March 2020

Arabinoxylan is the second most abundant component in the endosperm cell wall of barley and it has been shown to have negative effects on the viscosity and filtration rate of wort and beer. In this study, a glycoside hydrolase (GH) family 62 α-L-arabinofuranosidase (AFase), termed as TrAbf62A, was purified from the culture filtrate of Trichoderma reesei CICC 41495 by a combined chromatographic method. The preferred substrates of the purified TrAbf62A were soluble, highly substituted arabinoxylan oligosaccharides and polymers, similar to the type found in barley grain. TrAbf62A exhibited activity towards oligomeric and polymeric arabinoxylans, as well as colorimetric arabinose-based substrates, thus meeting the criteria to be classified as a type B AFase. TrAbf62A released mainly arabinose and xylose from soluble wheat arabinoxylan, thus indicating a dual lytic enzyme activity. Supplementation of TrAbf62A during mashing, with a loading of 12 mU/g malt, resulted in a 36.3% decrease in arabinoxylan polymer content, a 5.6% reduction in viscosity, and finally, a 22.1% increase in filtration rate. These results revealed that TrAbf62A has a high potential value in improving lautering performance during mashing.
Chemical and Physical Characteristics of Edible Films, Based on κ- and ι-Carrageenans with the Addition of Lapacho Tea Extract
Authors: Jancikova Simona , Dordevic Dani , Jamroz Ewelina , Behalova Hana , Tremlova Bohuslava
Published: March 2020

The aim of the study was to characterize antioxidant and textural property differences of edible films prepared with the addition of lapacho extract (LE). The experimentally produced edible films also contained different carrageenans (ι- and κ-carrageenan). The κ- and ι-carrageenan, glycerol and the different addition of LE (5%, 10%, 20%) were used as ingredients for forming films. The pH and viscosity were measured for film forming solutions (before drying). The following analyses were performed on films: the total polyphenol content (TPC), Ferric Reducing Antioxidant Power (FRAP) and 2-Diphenyl–1-Picrylhydrazyl (DPPH). Optical parameters were analyzed by the determination of UV-Vis spectra. The structure of films was characterized by scanning electron microscopy. The gained results indicated that the use of different gelling agents (ι- and κ-carrageenan) resulted in statistically significant (p < 0.05) differences in textural properties (strength and breaking strain) of produced edible films. The highest antioxidant properties and TPC had a κ film with 20% LE (DPPH: 87.63 ± 0.03%; TPC: 233.75 ± 0.104 mg gallic acid/g). According to these results, it can be concluded that edible films with the highest concentrations of added lapacho extract can serve as a good source of antioxidant compounds. Certainly, these properties can be usefully incorporated into the wrapped food commodity.
The Effect of Tobacco Smoking Differs across Indices of DNA Methylation-Based Aging in an African American Sample: DNA Methylation-Based Indices of Smoking Capture These Effects
Authors: Lei Man-Kit , Gibbons Frederick X. , Simons Ronald L. , Philibert Robert A. , Beach Steven R. H.
Published: March 2020

Smoking is one of the leading preventable causes of morbidity and mortality worldwide, prompting interest in its association with DNA methylation-based measures of biological aging. Considerable progress has been made in developing DNA methylation-based measures that correspond to self-reported smoking status. In addition, assessment of DNA methylation-based aging has been expanded to better capture individual differences in risk for morbidity and mortality. Untested to date, however, is whether smoking is similarly related to older and newer indices of DNA methylation-based aging, and whether DNA methylation-based indices of smoking can be used in lieu of self-reported smoking to examine effects on DNA methylation-based aging measures. In the current investigation we examine mediation of the impact of self-reported cigarette consumption on accelerated, intrinsic DNA methylation-based aging using indices designed to predict chronological aging, phenotypic aging, and mortality risk, as well as a newly developed DNA methylation-based measure of telomere length. Using a sample of 500 African American middle aged smokers and non-smokers, we found that a) self-reported cigarette consumption was associated with accelerated intrinsic DNA methylation-based aging on some but not all DNA methylation-based aging indices, b) for those aging outcomes associated with self-reported cigarette consumption, DNA methylation-based indicators of smoking typically accounted for greater variance than did self-reported cigarette consumption, and c) self-reported cigarette consumption effects on DNA methylation-based aging indices typically were fully mediated by DNA methylation-based indicators of smoking (e.g., PACKYRS from GrimAge; or cg05575921 CpG site). Results suggest that when DNA methylation-based indices of smoking are substituted for self-reported assessments of smoking, they will typically fully reflect the varied impact of cigarette smoking on intrinsic, accelerated DNA methylation-based aging.
YARS2 Missense Variant in Belgian Shepherd Dogs with Cardiomyopathy and Juvenile Mortality
Authors: Gurtner Corinne , Hug Petra , Kleiter Miriam , Köhler Kernt , Dietschi Elisabeth , Jagannathan Vidhya , Leeb Tosso
Published: March 2020

Dog puppy loss by the age of six to eight weeks after normal development is relatively uncommon. Necropsy findings in two spontaneously deceased Belgian Shepherd puppies indicated an abnormal accumulation of material in several organs. A third deceased puppy exhibited mild signs of an inflammation in the central nervous system and an enteritis. The puppies were closely related, raising the suspicion of a genetic cause. Pedigree analysis suggested a monogenic autosomal recessive inheritance. Combined linkage and homozygosity mapping assigned the most likely position of a potential genetic defect to 13 genome segments totaling 82 Mb. The genome of an affected puppy was sequenced and compared to 645 control genomes. Three private protein changing variants were found in the linked and homozygous regions. Targeted genotyping in 96 Belgian Shepherd dogs excluded two of these variants. The remaining variant, YARS2:1054G>A or p.Glu352Lys, was perfectly associated with the phenotype in a cohort of 474 Belgian Shepherd dogs. YARS2 encodes the mitochondrial tyrosyl-tRNA synthetase 2 and the predicted amino acid change replaces a negatively charged and evolutionary conserved glutamate at the surface of the tRNA binding domain of YARS2 with a positively charged lysine. Human patients with loss-of-function variants in YARS2 suffer from myopathy, lactic acidosis, and sideroblastic anemia 2, a disease with clinical similarities to the phenotype of the studied dogs. The carrier frequency was 27.2% in the tested Belgian Shepherd dogs. Our data suggest YARS2:1054G>A as the candidate causative variant for the observed juvenile mortality.
Grape-RNA: A Database for the Collection, Evaluation, Treatment, and Data Sharing of Grape RNA-Seq Datasets
Authors: Wang Yi , Zhang Rui , Liang Zhenchang , Li Shaohua
Published: March 2020

Since its inception, RNA sequencing (RNA-seq) has become the most effective way to study gene expression. After more than a decade of development, numerous RNA-seq datasets have been created, and the full utilization of these datasets has emerged as a major issue. In this study, we built a comprehensive database named Grape-RNA, which is focused on the collection, evaluation, treatment, and data sharing of grape RNA-seq datasets. This database contains 1529 RNA-seq samples, 112 microRNA samples from the public platform, and 485 RNA-seq in-house datasets sequenced by our lab. We classified these data into 25 conditions and provide the sample information, cleaned raw data, expression level, assembled unigenes, useful tools, and other relevant information to the users. Thus, this study provides data and tools that should be beneficial for researchers by allowing them to easily use the RNA-seq. The provided information can greatly contribute to grape breeding and genomic and biological research. This study may improve the usage of RNA-seq.
Comparative Transcriptomics Reveals Clues for Differences in Pathogenicity between Hysterothylacium aduncum, Anisakis simplex sensu stricto and Anisakis pegreffii
Authors: Cavallero Serena , Lombardo Fabrizio , Salvemini Marco , Pizzarelli Antonella , Cantacessi Cinzia , D’Amelio Stefano
Published: March 2020

Ascaridoid nematodes are widespread in marine fishes. Despite their major socioeconomic importance, mechanisms associated to the fish-borne zoonotic disease anisakiasis are still obscure. RNA-Seq and de-novo assembly were herein applied to RNA extracted from larvae and dissected pharynx of Hysterothylacium aduncum (HA), a non-pathogenic nematode. Assembled transcripts in HA were annotated and compared to the transcriptomes of the zoonotic species Anisakis simplex sensu stricto (AS) and Anisakis pegreffii (AP). Approximately 60,000,000 single-end reads were generated for HA, AS and AP. Transcripts in HA encoded for 30,254 putative peptides while AS and AP encoded for 20,574 and 20,840 putative peptides, respectively. Differential gene expression analyses yielded 471, 612 and 526 transcripts up regulated in the pharynx of HA, AS and AP. The transcriptomes of larvae and pharynx of HA were enriched in transcripts encoding collagen, peptidases, ribosomal proteins and in heat-shock motifs. Transcripts encoding proteolytic enzymes, anesthetics, inhibitors of primary hemostasis and virulence factors, anticoagulants and immunomodulatory peptides were up-regulated in AS and AP pharynx. This study represents the first transcriptomic characterization of a marine parasitic nematode commonly recovered in fish and probably of negligible concern for public health.
Identification of Genic SSRs Provide a Perspective for Studying Environmental Adaptation in the Endemic Shrub Tetraena mongolica
Authors: Dang Zhenhua , Huang Lei , Jia Yuanyuan , Lockhart Peter J. , Fong Yang , Tian Yunyun
Published: March 2020

Tetraena mongolica is a xerophytic shrub endemic to desert regions in Inner Mongolia. This species has evolved distinct survival strategies that allow it to adapt to hyper-drought and heterogeneous habitats. Simple sequence repeats (SSRs) may provide a molecular basis in plants for fast adaptation to environmental change. Thus, identifying SSRs and their possible effects on gene behavior has the potential to provide valuable information for studies of adaptation. In this study, we sequenced six individual transcriptomes of T. mongolica from heterogeneous habitats, focused on SSRs located in genes, and identified 811 polymorphic SSRs. Of the identified SSRs, 172, 470, and 76 were located in 5′ UTRs, CDSs, and 3′ UTRs in 591 transcripts; and AG/CT, AAC/GTT, and AT/AT were the most abundant repeats in each gene region. Functional annotation showed that many of the identified polymorphic SSRs were in genes that were enriched in several GO terms and KEGG pathways, suggesting the functional significance of these genes in the environmental adaptation process. The identification of polymorphic genic SSRs in our study lays a foundation for future studies investigating the contribution of SSRs to regulation of genes in natural populations of T. mongolica and their importance for adaptive evolution of this species.
Crosstalk between Dendritic Cells and Immune Modulatory Agents against Sepsis
Authors: Wang Guoying , Li Xianghui , Zhang Lei , Elgaili Abdalla Abualgasim , Teng Tieshan , Li Yanzhang
Published: March 2020

Dendritic cells (DCs) play a critical role in the immune system which sense pathogens and present their antigens to prime the adaptive immune responses. As the progression of sepsis occurs, DCs are capable of orchestrating the aberrant innate immune response by sustaining the Th1/Th2 responses that are essential for host survival. Hence, an in-depth understanding of the characteristics of DCs would have a beneficial effect in overcoming the obstacle occurring in sepsis. This paper focuses on the role of DCs in the progression of sepsis and we also discuss the reverse sepsis-induced immunosuppression through manipulating the DC function. In addition, we highlight some potent immunotherapies that could be used as a novel strategy in the early treatment of sepsis.
Assessment of Geological, Hydrogeological and Geotechnical Characteristics of a Proposed Waste Disposal Site: A Case Study in Khon Kaen, Thailand
Authors: Arjwech Rungroj , Somchat Kittipong , Pondthai Potpreecha , Everett Mark , Schulmeister Marcia , Saengchomphu Sakhon
Published: March 2020

Occasionally, a selected site suitable for landfill construction is severely protested against by locals. This issue can cause the proposed landfill to be relocated to an environmentally sensitive area. The proposed Khon Kaen waste disposal site has been planned as an integrated municipal solid waste management system, although the site is situated in an environmentally sensitive area. A site assessment can guarantee the suitability of waste disposal construction, with procedures that aim to assess the potential of geological and hydrogeological characteristics, geological barriers, geotechnical properties of material for landfill construction and groundwater conditions for future monitoring of such facilities. The study area is located on foothills where no geohazard or seismic impacts have been recorded. The geology is composed of sandstone, siltstone, and mudstone bedrocks mostly overlain by unconsolidated sediments. The natural geological barriers are clay and regolith. The clay layer lies locally and is rather thin, at around 2–3 m thickness. The study area is situated in an area that is highly vulnerable to groundwater pollution. The distinct weaknesses of this site along the foothill are a prominent transport path of shallow flows; high groundwater fluctuation, especially during the rainy season; that it is a recharge area with a high fracture zone; and the high permeability of colluvium. The material characteristics in the site make it suitable for use as landfill cover and liner. Following compaction, the coefficient of permeability ranges from 1.2 × 10−7 to 7.1 × 10−7 cm/s, which is acceptably impervious.
Associations of Frailty Status with Low-Mileage Driving and Driving Cessation in a Cohort of Older Drivers
Authors: Crowe Christopher L. , Kannoth Sneha , Andrews Howard , Strogatz David , Li Guohua , DiGuiseppi Carolyn , Hill Linda , Eby David W. , Molnar Lisa J. , Mielenz Thelma J.
Published: March 2020

The US older adult population is projected to considerably increase in the future, and continued driving mobility is important for health aspects in populations with fewer transportation alternatives. This study evaluated whether frailty is associated with low-mileage driving (<1865 miles per year) and driving cessation among older adults. Baseline demographics and health data were collected for 2990 older drivers via in-person assessments and questionnaires, with 2964 reporting baseline frailty data. Multivariable log-binomial regression models were used to evaluate the association between baseline frailty status and low-mileage driving. Multivariable Cox proportional hazards regression were used to evaluate the association between baseline frailty status and driving cessation. For every unit increase in frailty, the estimated adjusted risk of driving fewer than 1865 miles/year increased by 138% (adjusted risk ratio: 2.38, 95% CI: 1.63–3.46). Relative to older drivers who were not frail, the adjusted hazard ratios of driving cessation were 4.15 (95% CI: 1.89–9.10) for those classified as prefrail and 6.08 (95% CI: 1.36–27.26) for those classified as frail. Frailty is positively associated with low-mileage driving status and driving cessation in a dose-response fashion. Public health interventions that reduce frailty, such as physical activity, may help older drivers maintain safe and independent mobility.