bluuebunny/crossref_metadata_2025 huggingface dataset:
Heritability Estimation and Environmental Risk Assessment for Type 2 Diabetes Mellitus in a Rural Region in Henan, China: Family-Based and Case-Control Studies
Authors: Feng Yinhua , Li Xing , Mao Zhenxing , Huo Wenqian , Hou Jian , Wang Chongjian , Li Wenjie , Yu Songcheng
Published: July 2021

Objective: The prevalence of type 2 diabetes mellitus (T2DM) varies greatly in different regions and populations. This study aims to assess the heritability and environmental risk factors of T2DM among rural Chinese adults.

Methods: Thousand five hundred thirty three participants from 499 extended families, which included 24 nuclear families, were recruited in the family-based study to assess the heritable risk of T2DM. Heritability of T2DM was estimated by the Falconer method. Using conditional logistic regression model, couple case-control study involving 127 couples were applied to assess the environmental risk factors of T2DM.

Results: Compared with the Henan Rural Cohort, T2DM was significantly clustered in the nuclear families (OR: 8.389, 95% CI: 5.537–12.711, P < 0.001) and heritability was 0.74. No association between the heredity of T2DM and sex was observed between the extended families and the Henan Rural Cohort. Besides, results from the couple case-control study showed that physical activity (OR: 0.482, 95% CI: 0.261–0.893, P = 0.020) and fat intake (OR: 3.036, 95% CI: 1.070–8.610, P = 0.037) was associated with T2DM, and the proportion of offspring engaged in medium and high physical activity was higher than that of mothers in mother-offspring pairs.

Conclusion: People with a family history of T2DM may have a higher risk of developing T2DM, however, there was no difference in genetic risk between males and females. Adherence to active physical activity and low fat intake can reduce the risk of T2DM.
Computer Vision Syndrome During SARS-CoV-2 Outbreak in University Students: A Comparison Between Online Courses and Classroom Lectures
Authors: Wang Lixiang , Wei Xin , Deng Yingping
Published: July 2021

Purpose: To compare the prevalence of computer vision syndrome in university students of different teaching modes during the SARS-CoV-2 outbreak period.

Methods: A cross-sectional survey study using the validated Computer Vision Syndrome Questionnaire in Chinese medical students of Sichuan University who took classroom lectures and the same-grade foreign students from a Bachelor of Medicine and Bachelor of Surgery (MBBS) program who took online lectures with similar schedules.

Results: A total of 137 responses from 63 Chinese students and 74 MBBS students were obtained. The highest frequency of digital screen time was 7-9 h (43.24%, 32/74) for MBBS students and 2-4 h (46.03%, 29/63) for Chinese students. The prevalence of computer vision syndrome among Chinese students and MBBS students were 50.79% and 74.32%, respectively (P = 0.004). The average numbers of reported symptoms were 5.00 ± 2.17 in Chinese students and 5.91 ± 1.90 in MBBS students (P = 0.01). The three most highly reported symptoms were “heavy eyelids” (53.97%), “dryness” (50.79%), and “feeling of a foreign body” (46.03%) in Chinese students and “dryness” (72.97%), “feeling of a foreign body” (62.16%), and “heavy eyelids” (58.11%) in MBBS students. The sum grades of computer vision syndrome had a moderate positive correlation with screen time (Spearman's correlation coefficient = 0.386, P < 0.001). The grades of symptoms of “feeling of a foreign body,” “heavy eyelids,” and “dryness” showed a weak positive correlation with screen time (Spearman's correlation coefficients were 0.220, 0.205, and 0.230, respectively).

Conclusion: Online study may contribute to the prevalence of computer vision syndrome among university students.
Perceived Barriers to Blood Flow Restriction Training
Authors: Rolnick Nicholas , Kimbrell Kyle , Cerqueira Mikhail Santos , Weatherford Ben , Brandner Christopher
Published: July 2021

Blood flow restriction (BFR) training is increasing in popularity in the fitness and rehabilitation settings due to its role in optimizing muscle mass and strength as well as cardiovascular capacity, function, and a host of other benefits. However, despite the interest in this area of research, there are likely some perceived barriers that practitioners must overcome to effectively implement this modality into practice. These barriers include determining BFR training pressures, access to appropriate BFR training technologies for relevant demographics based on the current evidence, a comprehensive and systematic approach to medical screening for safe practice and strategies to mitigate excessive perceptual demands of BFR training to foster long-term compliance. This manuscript attempts to discuss each of these barriers and provides evidence-based strategies and direction to guide clinical practice and future research.
Hurry Up, We Need to Find the Key! How Regulatory Focus Design Affects Children’s Trust in a Social Robot
Authors: Calvo-Barajas Natalia , Elgarf Maha , Perugia Giulia , Paiva Ana , Peters Christopher , Castellano Ginevra
Published: July 2021

In educational scenarios involving social robots, understanding the way robot behaviors affect children’s motivation to achieve their learning goals is of vital importance. It is crucial for the formation of a trust relationship between the child and the robot so that the robot can effectively fulfill its role as a learning companion. In this study, we investigate the effect of a regulatory focus design scenario on the way children interact with a social robot. Regulatory focus theory is a type of self-regulation that involves specific strategies in pursuit of goals. It provides insights into how a person achieves a particular goal, either through a strategy focused on “promotion” that aims to achieve positive outcomes or through one focused on “prevention” that aims to avoid negative outcomes. In a user study, 69 children (7–9 years old) played a regulatory focus design goal-oriented collaborative game with the EMYS robot. We assessed children’s perception of likability and competence and their trust in the robot, as well as their willingness to follow the robot’s suggestions when pursuing a goal. Results showed that children perceived the prevention-focused robot as being more likable than the promotion-focused robot. We observed that a regulatory focus design did not directly affect trust. However, the perception of likability and competence was positively correlated with children’s trust but negatively correlated with children’s acceptance of the robot’s suggestions.
Optimal Operations Management of Mobility-on-Demand Systems
Authors: Wollenstein-Betech Salomón , Paschalidis Ioannis Ch. , Cassandras Christos G.
Published: July 2021

The emergence of the sharing economy in urban transportation networks has enabled new fast, convenient and accessible mobility services referred to as Mobilty-on-Demand systems (e.g., Uber, Lyft, DiDi). These platforms have flourished in the last decade around the globe and face many operational challenges in order to be competitive and provide good quality of service. A crucial step in the effective operation of these systems is to reduce customers' waiting time while properly selecting the optimal fleet size and pricing policy. In this paper, we jointly tackle three operational decisions: (i) fleet size, (ii) pricing, and (iii) rebalancing, in order to maximize the platform's profit or its customers' welfare. To accomplish this, we first devise an optimization framework which gives rise to a static policy. Then, we elaborate and propose dynamic policies that are more responsive to perturbations such as unexpected increases in demand. We test this framework in a simulation environment using three case studies and leveraging traffic flow and taxi data from Eastern Massachusetts, New York City, and Chicago. Our results show that solving the problem jointly could increase profits between 1% and up to 50%, depending on the benchmark. Moreover, we observe that the proposed fleet size yield utilization of the vehicles in the fleet is around 75% compared to private vehicle utilization of 5%.
The Peak Absorbance Wavelength of Photosynthetic Pigments Around Other Stars From Spectral Optimization
Authors: Lehmer Owen R. , Catling David C. , Parenteau Mary N. , Kiang Nancy Y. , Hoehler Tori M.
Published: July 2021

In the search for life on other planets, the presence of photosynthetic surface vegetation may be detectable from the colors of light it reflects. On the modern Earth, this spectral reflectance is characterized by a steep increase in reflectance between the red and near‐infrared wavelengths, a signature known as the “red edge”. This edge-like signature occurs at wavelengths of peak photon absorbance, which are the result of adaptations of the phototroph to their spectral environment. On planets orbiting different stellar types, red edge analogs may occur at other colors than red. Thus, knowing the wavelengths at which photosynthetic organisms preferentially absorb and reflect photons is necessary to detect red edge analogs on other planets. Using a numerical model that predicts the absorbance spectrum of extant photosynthetic pigments on Earth from Marosvölgyi and van Gorkom (2010), we calculate the absorbance spectrum for pigments on an Earth-like planet around F through late M type stars that are adapted for maximal energy production. In this model, cellular energy production is maximized when pigments are tuned to absorb at the wavelength that maximizes energy input from incident photons while minimizing energy losses due to thermal emission and building cellular photosynthetic apparatus. We find that peak photon absorption for photosynthetic organisms around F type stars tends to be in the blue while for G, K, and early M type stars, red or just beyond is preferred. Around the coolest M type stars, these organisms may preferentially absorb in the near-infrared, possibly past one micron. These predictions are consistent with previous, qualitative estimates of pigment absorptance. Our predicted absorbance spectra for photosynthetic surface organisms depend on both the stellar type and planetary atmospheric composition, especially atmospheric water vapor concentrations, which alter the availability of surface photons and thus the predicted pigment absorption. By constraining the absorbance spectra of alien, photosynthetic organisms, future observations may be better equipped to detect the weak spectral signal of red edge analogs.
Use of Polyphenols as Modulators of Food Allergies. From Chemistry to Biological Implications
Authors: Bessa Catarina , Francisco Telmo , Dias Ricardo , Mateus Nuno , Freitas Victor de , Pérez-Gregorio Rosa
Published: July 2021

The multifactorial process of aging predisposes humans to infections and inflammatory disorders, thus affecting their quality of life and longevity. Given this reality, the need to increase the consumption of bioactive compounds, like dietary polyphenols emerges in our daily basis mostly due to their health related effects in slowing-down the incidence of chronic and degenerative diseases and even food allergy, which has been growing rapidly in prevalence currently affecting 5% of adults and 8% of children. Polyphenols embrace a large family of secondary metabolites from plant-derived foods and food wastes and are considerable of interest since they have attracted special attention over the years because of their reported anti-inflammatory and antimicrobial properties along with their high antioxidant capacity. These compounds are claimed as nutraceuticals with protective effect in offsetting oxidant species over-genesis in normal cells, and with the potential ability to stop or reverse oxidative stress-related diseases. Plant-derived foods represent a substantive portion of human diet containing a significant amount of structurally diverse polyphenols. There is a need to understand the polyphenolic composition of plant-derived foods mainly because of its chemistry, which discloses the bioactivity of a plant extract. However, the lack of standardized methods for analysis and other difficulties associated to the nature and distribution of plant polyphenols leads to a high variability of available data. Furthermore, there is still a gap in the understanding of polyphenols bioavailability and pharmacokinetics, which clearly difficult the settlement of the intake needed to observe health outcomes. Many efforts have been made to provide highly sensitive and selective analytical methods for the extraction (liquid-liquid; solid-liquid; supercritical-fluid), separation (spectrophotometric methods) and structural identification (chromatographic techniques, NMR spectroscopy, MS spectrometry) of phenolic and polyphenolic compounds present in these extracts. Liquid chromatography coupled to mass spectrometry (LC-MS) has been a fundamental technique in this area of research, not only for the determination of this family of compounds in food matrices, but also for the characterization and identification of new polyphenols classified with nutraceutical interest. This review summarizes the nature, distribution and main sources of polyphenols, analytical methods from extraction to characterization to further evaluate the health effects toward immune reactions to food.
Case Report: A Rare Case of Fourth Ventricle to Spinal Subarachnoid Space Shunt Migration: Surgical Pearl and Literature Review
Authors: Serratrice Nicolas , Faddoul Joe , Tarabay Bilal , Taifour Sarkis , Abi Lahoud Georges Naïm
Published: July 2021

Background: In the event of syringomyelia communicating with the fourth ventricle, a fourth ventricle to cervical subarachnoid space shunting could be proposed.

Case Report: In this review article, we describe the case of a 40-year-old woman who had a previously implanted fourth ventricle to spinal subarachnoid space shunt for the treatment of syringomyelia in the context of Chiari syndrome. The catheter migrated intradurally to the lumbosacral space, but in the absence of neurological repercussions, we decided to leave it in place.

Conclusions: To the best of our knowledge, this is the first case described in the literature review of a catheter migration in the subarachnoid space from occipitocervical to lumbosacral level.
Antimicrobial Resistance Pilot Surveillance of Pigs and Chickens in Vietnam, 2017–2019
Authors: Tuat C. V. , Hue P. T. , Loan N. T. P. , Thuy N. T. , Hue L. T. , Giang V. N. , Erickson Vera I. , Padungtod Pawin
Published: July 2021

Antimicrobial use (AMU) and antimicrobial resistance (AMR) are a growing public health and economic threat in Vietnam. We conducted a pilot surveillance programme in five provinces of Vietnam, two in the south and three in the north, to identify antimicrobial resistance (AMR) in rectal swab samples from pigs and fecal samples from chickens at slaughter points during three different points in time from 2017 to 2019.Escherichia coli(E. coli) and non-typhoidalSalmonella(NTS) isolates were tested for antimicrobial susceptibility using disk diffusion assay for 19 antimicrobial agents belonging to nine antimicrobial classes and Etest for colistin (polymyxin). Almost allE. coli(99%; 1029/1042) and NTS (96%; 208/216) isolates were resistant to at least one antimicrobial agent; 94% (981/1042) ofE. coliand 89% (193/216) of NTS isolates were multidrug-resistant (MDR). Higher proportions ofE. coliand NTS isolated from chickens were resistant to all antimicrobial classes than those isolates from pigs. There was a significantly higher proportion of MDR NTS isolates from the southern provinces of Ho Chi Minh City and Long An (p= 0.008). Although there were increasing trends of NTS in proportion of resistance to fluoroquinolone over the three surveillance rounds, there was a significant decreasing trend of NTS in proportion of resistance to polymyxin (p= 0.002). It is important to establish an annual AMR surveillance program for livestock in Vietnam to assess the impact of interventions, observe trends and drive decision making that ultimately contributes to reducing AMR public health threat.
Detection and Genetic Diversity of a Novel Water Buffalo Astrovirus Species Found in the Guangxi Province of China
Authors: Fang Qingli , Li Mingyang , Liu Haifeng , Chen Kuirong , Du Yanjie , Wen Chongli , Wei Yingyi , Ouyang Kang , Wei Zuzhang , Chen Ying , Huang Weijian
Published: July 2021

Astroviruses (AstVs) are major causative agents of gastroenteritis and have been detected worldwide. Little is known about the prevalence of neurotropic AstVs in Chinese water buffaloes, but a novel species which is associated with encephalitis and meningitis has recently been found. In this study, based on nested RT-PCR, rapid amplification of the 3′-cDNA end (3′-RACE) and next-generation sequencing (NGS), we examined the infection of AstVs in water buffaloes in the Guangxi Province of China. The results showed that the AstV infection was found in 40% (6/15) of the farms examined, and the prevalence of AstV in their feces was 11% (33/297). In addition, two near-full-length and two complete open reading frame 2 (ORF2) genes of AstVs from fecal sources were sequenced. Phylogenetic analysis of the ORF2 sequences indicated three lineages of BufAstVs, BufAstV lineage 1 was close related to the BoAstV, lineage 2 was related to the BufAstVs, and lineage 3 was classified as novel AstVs, which had a close relationship with the neurotropic/neurovirulent AstVs strains found in bovine, ovine, and musks. Moreover, genomic a recombination between the BufAstV and BoAstV strains was identified. This is a novel study reporting the genetic diversity of BufAstV infection in China especially found the similar neurotropic strains from fecal sources of water buffaloes, and it also provides details of the epidemiology, genetic recombination, and interspecies transmission of BoAstV and BufAstV in water buffaloes from the Guangxi Province of China.
Cotton Gin Stand Machine-Vision Inspection and Removal System for Plastic Contamination: Software Design
Authors: Pelletier Mathew G. , Holt Greg A. , Wanjura John D.
Published: July 2021

The removal of plastic contamination from cotton lint is an issue of top priority to the U.S. cotton industry. One of the main sources of plastic contamination showing up in marketable cotton bales is plastic used to wrap cotton modules produced by John Deere round module harvesters. Despite diligent efforts by cotton ginning personnel to remove all plastic encountered during module unwrapping, plastic still finds a way into the cotton gin’s processing system. To help mitigate plastic contamination at the gin, a machine-vision detection and removal system was developed that utilizes low-cost color cameras to see plastic coming down the gin-stand feeder apron, which upon detection, blows plastic out of the cotton stream to prevent contamination. This paper presents the software design of this inspection and removal system. The system was tested throughout the entire 2019 cotton ginning season at two commercial cotton gins and at one gin in the 2018 ginning season. The focus of this report is to describe the software design and discuss relevant issues that influenced the design of the software.
Quality Appraisal of Ambulatory Oral Cephalosporin and Fluoroquinolone Use in the 16 German Federal States from 2014–2019
Authors: Gradl Gabriele , Werning Johanna , Enners Salka , Kieble Marita , Schulz Martin
Published: July 2021

Background: Despite concerns about causing bacterial resistance and serious side effects, oral cephalosporins and fluoroquinolones are still frequently prescribed in Germany. We aimed to test a method for the detection of regional quality differences in the use of oral cephalosporins and fluoroquinolones and to apply this to the German federal states. Methods: Use of antibiotics from 2014–2019 was analyzed using dispensing data from community pharmacies claimed to the statutory health insurance (SHI) funds. Quality of regional antibiotic use in 2019 was assessed by calculating indicators based on defined daily doses per 1000 SHI-insured persons per day (DID). Oral cephalosporin and fluoroquinolone use was followed by linear regression analyses. Results: The method used was suitable to find meaningful quality differences in ambulatory oral cephalosporin and fluoroquinolone use between the German federal states. In 2019, DID varied from 1.62 in Brandenburg to 3.17 in Rhineland-Palatinate for cephalosporins and from 0.47 in Brandenburg to 0.89 in Saarland for fluoroquinolones. The city-states Hamburg, Bremen, and Berlin showed highest quality with the applied indicator set. From 2014–2019, a significant decrease in utilization of oral cephalosporins was found in all federal states. During 2017–2019, all states showed a significant decline of fluoroquinolone use.
Characterization of the Striatal Extracellular Matrix in a Mouse Model of Parkinson’s Disease
Authors: Freitas Ana , Aroso Miguel , Barros António , Fernández Miriam , Conde-Sousa Eduardo , Leite Marina , Carvalho Eva Daniela , Ribeiro Cristina C , Ferreira Rita , Pêgo Ana Paula , Vitorino Rui , Gomez-Lazaro Maria
Published: July 2021

Parkinson’s disease’s etiology is unknown, although evidence suggests the involvement of oxidative modifications of intracellular components in disease pathobiology. Despite the known involvement of the extracellular matrix in physiology and disease, the influence of oxidative stress on the matrix has been neglected. The chemical modifications that might accumulate in matrix components due to their long half-live and the low amount of extracellular antioxidants could also contribute to the disease and explain ineffective cellular therapies. The enriched striatal extracellular matrix from a mouse model of Parkinson’s disease was characterized by Raman spectroscopy. We found a matrix fingerprint of increased oxalate content and oxidative modifications. To uncover the effects of these changes on brain cells, we morphologically characterized the primary microglia used to repopulate this matrix and further quantified the effects on cellular mechanical stress by an intracellular fluorescence resonance energy transfer (FRET)-mechanosensor using the U-2 OS cell line. Our data suggest changes in microglia survival and morphology, and a decrease in cytoskeletal tension in response to the modified matrix from both hemispheres of 6-hydroxydopamine (6-OHDA)-lesioned animals. Collectively, these data suggest that the extracellular matrix is modified, and underscore the need for its thorough investigation, which may reveal new ways to improve therapies or may even reveal new therapies.
Thermal Physics and Glaucoma II: Preliminary Evidences for a Thermophysical Design of a Possible Visible-Light-Photons Therapy
Authors: Grisolia Giulia , Astori Mariarosa , Ponzetto Antonio , Vercesi Antonio , Lucia Umberto
Published: July 2021

Recently, a non-equilibrium thermodynamic approach has been developed in order to model the fundamental role of the membrane electric potential in the cell behaviour. A related new viewpoint is introduced, with a design of a photobiomodulation treatment in order to restore part of the visual field. Here, a first step in experimental evidence of the validity of the thermodynamic approach is developed. This result represents the starting point for future experimental improvements for light stimulation in order to improve the quality of life of the patients. The future possible therapy will be in addition to the pharmacological treatments.
Controlling Surface Plasmon Polaritons Propagating at the Boundary of Low-Dimensional Acoustic Metamaterials
Authors: Ioannidis Thanos , Gric Tatjana , Rafailov Edik
Published: July 2021

As a novel type of artificial media created recently, metamaterials demonstrate novel performance and consequently pave the way for potential applications in the area of functional engineering in comparison to the conventional substances. Acoustic metamaterials and plasmonic structures possess a wide variety of exceptional physical features. These include effective negative properties, band gaps, negative refraction, etc. In doing so, the acoustic behaviour of conventional substances is extended. Acoustic metamaterials are considered as the periodic composites with effective parameters that might be engineered with the aim to dramatically control the propagation of supported waves. Homogenization of the system under consideration should be performed to seek the calculation of metamaterial permittivity. The dispersion behaviour of surface waves propagating from the boundary of a nanocomposite composed of semiconductor enclosures that are systematically distributed in a transparent matrix and low-dimensional acoustic metamaterial and constructed by an array of nanowires implanted in a host material are studied. We observed the propagation of surface plasmon polaritons. It is demonstrated that one may dramatically modify the properties of the system by tuning the geometry of inclusions.
The 3D Printing of Freestanding PLLA Thin Layers and Improving First Layer Consistency through the Introduction of Sacrificial PVA
Authors: Roper David M. , Kwon Kyung-Ah , Best Serena M. , Cameron Ruth E.
Published: July 2021

Fused filament fabrication (FFF) is an inexpensive way of producing objects through a programmed layer-by-layer deposition. For multi-layer, macro-scaled prints, acceptable printing is achieved provided, amongst other factors, first layer adhesion is sufficient to fix a part to the surface during printing. However, in the deposition of structures with a single or few layers, first layer consistency is significantly more important and is an issue that has been previously overlooked. As layer-to-bed adhesion is prioritised in first layer printing, thin layer structures are difficult to remove without damage. The deposition of controllable thin structures has potential in tissue engineering through the use of bioactive filaments and incorporation of microfeatures into complex, patient-specific scaffolds. This paper presents techniques to progress the deposition of thin, reproducible structures. The linear thickness variation of 3D-printed single PVA and PLLA layers is presented as a function of extrusion factor and the programmed vertical distance moved by the nozzle between layers (the layer separation). A sacrificial PVA layer is shown to significantly improve first layer consistency, reducing the onus on fine printer calibration in the deposition of single layers. In this way, the linear variation in printed single PLLA layers with bed deviation is drastically reduced. Further, this technique is used to demonstrate the printing of freestanding thin layers of ~25 µm in thickness.
Single Mode, Air-Cladded Optical Waveguides Supported by a Nano-Fin Fabricated with Direct Laser Writing
Authors: Gill Parvinder K. , Marom Dan M.
Published: July 2021

Single-mode, air-cladded optical waveguides have wavelength scale diameters, making them very fragile and difficult to handle and yet highly desirable for sensing and inter-chip photonic interconnects. These contradictory qualities are resolved in this work by supporting the optical waveguide with a nano-fin structure attached to a substrate, narrow enough and sufficiently tall to minimally impact the wave-guiding metrics of the solid core while providing structural mechanical integrity. The design considerations for the nano-fin-supported waveguide and its realization using a commercial direct laser writing system based on two-photon activation of a photopolymer is reported herein. The 3D printed waveguides are characterized and experimentally assessed, demonstrating low birefringence and an estimated propagation loss for LP01x and LP01y of 2.9 dB/mm and 3.4 dB/mm, respectively, attributed to surface roughness and the relatively high refractive index contrast with air.
Impact Study of Temperature on the Time Series Electricity Demand of Urban Nepal for Short-Term Load Forecasting
Authors: Rajbhandari Yaju , Marahatta Anup , Ghimire Bishal , Shrestha Ashish , Gachhadar Anand , Thapa Anup , Chapagain Kamal , Korba Petr
Published: July 2021

Short-term electricity demand forecasting is one of the best ways to understand the changing characteristics of demand that helps to make important decisions regarding load flow analysis, preventing imbalance in generation planning, demand management, and load scheduling, all of which are actions for the reliability and quality of that power system. The variation in electricity demand depends upon various parameters, such as the effect of the temperature, social activities, holidays, the working environment, and so on. The selection of improper forecasting methods and data can lead to huge variations and mislead the power system operators. This paper presents a study of electricity demand and its relation to the previous day’s lags and temperature by examining the case of a consumer distribution center in urban Nepal. The effect of the temperature on load, load variation on weekends and weekdays, and the effect of load lags on the load demand are thoroughly discussed. Based on the analysis conducted on the data, short-term load forecasting is conducted for weekdays and weekends by using the previous day’s demand and temperature data for the whole year. Using the conventional time series model as a benchmark, an ANN model is developed to track the effect of the temperature and similar day patterns. The results show that the time series models with feedforward neural networks (FF-ANNs), in terms of the mean absolute percentage error (MAPE), performed better by 0.34% on a weekday and by 8.04% on a weekend.
Observed and Future Precipitation and Evapotranspiration in Water Management Zones of Uganda: CMIP6 Projections
Authors: Onyutha Charles , Asiimwe Arnold , Ayugi Brian , Ngoma Hamida , Ongoma Victor , Tabari Hossein
Published: July 2021

We used CMIP6 GCMs to quantify climate change impacts on precipitation and potential evapotranspiration (PET) across water management zones (WMZs) in Uganda. Future changes are assessed based on four Shared Socioeconomic Pathways (SSP) scenarios including SSP1-2.6, SSP2-4.5, SSP3-7.0, and SSP5-8.5 over the periods 2021–2040, 2041–2060, 2061–2080, and 2081–2100. Both precipitation and PET are generally projected to increase across all the WMZs. Annual PET in the 2030s, 2050s, 2070s, 2090s will increase in the ranges 1.1–4.0%, 4.8–7.9%, 5.1–11.8%, and 5.3–17.1%, respectively. For the respective periods, annual precipitation will increase in the ranges 4.0–7.8%, 7.8–12.5%, 7.9–19.9%, and 6.9–26.3%. The lower and upper limits of these change ranges for both precipitation and PET are, respectively, derived under SSP1-2.6 and SSP5-8.5 scenarios. Climate change will impact on PET or precipitation disproportionately across the WMZs. While the eastern WMZ (Kyoga) will experience the largest projected precipitation increase especially towards the end of the century, the southern WMZ (Victoria) exhibited the largest PET increase. Our findings are relevant for understanding hydrological impacts of climate change across Uganda, in the background of global warming. Thus, the water sector should devise and implement adaptation measures to impede future socioeconomic and environmental crises in the country.
Co-Occurrence of Marine Extremes Induced by Tropical Storms and an Ocean Eddy in Summer 2016: Anomalous Hydrographic Conditions in the Pacific Shelf Waters off Southeast Hokkaido, Japan
Authors: Kuroda Hiroshi , Taniuchi Yukiko , Kasai Hiromi , Nakanowatari Takuya , Setou Takashi
Published: July 2021

This study proposes an analysis methodology to address how very rare marine extremes can be understood using limited data. In summer 2016, extreme weather and marine events occurred simultaneously around the Pacific shelf off southeastern Hokkaido, Japan. Six successive tropical storms brought extreme precipitation and an anticyclonic mesoscale eddy of subtropical Kuroshio water closely approached the coast, locally causing marine heat waves. We examined how these compound extremes affected oceanographic conditions on the coastal shelf by analyzing data from ship surveys in October 2016 on the Pacific shelf and outputs from a realistic ocean model. Climatologically, warm, high-salinity (33.0–33.7) subtropical water from the Okhotsk Sea (modified Soya Warm Current water) is distributed near the sea surface on the Pacific shelf in October and transported by the along-shelf boundary current. In 2016, however, a vertically well-mixed low-salinity (<33.0) layer associated with the heavy rainfall was observed at 0–50 m depth on the shelf, salinity maxima (≥33.7) associated with Kuroshio water from the mesoscale eddy occurred at 50–150 m depth on the slope, and baroclinic jets formed along the salinity front near the shelfbreak. These observed salinity structures were reproduced by a 1/50° ocean model. Particle-tracking experiments revealed that the low-salinity water originated mainly off eastern Hokkaido, where heavy rainfall events occurred in August, and was modified by mixing with Soya Warm Current water before transport to the Pacific shelf.
Capturing Multiple Disease Resistance in Wheat through Intergeneric Hybridization
Authors: Fedak George , Chi Dawn , Hiebert Colin , Fetch Tom , McCallum Brent , Xue Allen , Cao Wenguang
Published: July 2021

Derivatives from 4 species from the secondary gene pool of wheat—1 diploid (T. monococcum), 2 tetraploid (T. carthlicum; T. timopheevi), and 1 hexaploid (T. miguschovae)—were screened for resistance to Fusarium head blight, leaf rust, stem rust, and stripe rust. Where screening, genetic studies, and mapping were completed it was shown that all species carried resistance to multiple plant diseases. Some derived lines carried resistance to up to four different diseases. Where mapping was completed, it was shown that different diseases mapped to different chromosomes within any one accession.
Optimal Woven EndoBridge (WEB) Device Size Selection Using Automated Volumetric Software
Authors: Ansari Sameer , Zevallos Cynthia B. , Farooqui Mudassir , Dajles Andres , Schafer Sebastian , Quispe-Orozco Darko , Mendez-Ruiz Alan , Abdelkarim Samir , Dandapat Sudeepta , Ortega-Gutierrez Santiago
Published: July 2021

Introduction: Selecting the appropriate Woven EndoBridge (WEB) device sizing for the treatment of wide-neck bifurcation aneurysms (WNBAs) remains challenging. The aim of this study was to evaluate different volumetric-based imaging methodologies to predict an accurate WEB device size selection to result in a successful implantation. Methods: All consecutive patients treated with WEB devices for intracranial aneurysms from January 2019 to June 2020 were included. Aneurysm dimensions to calculate aneurysm volumes were measured using three different modalities: automated three-dimensional (3D) digital subtraction angiography (DSA), manual 3D DSA, and two-dimensional (2D) DSA. The device–aneurysm volume (DAV) ratio was defined as device volume divided by the aneurysm volume. WEB volumes and the DAV ratios were used for assessing the device implantation success and follow-up angiographic outcomes at six months. Pearson correlation, Wilcoxon Rank Sum test, and density approximations were used for estimating the WEB volumes and the imaging modality volumes for successful implantation. Results: A total of 41 patients with 43 aneurysms were included in the study. WEB device and aneurysm volume correlation coefficient was highest for 3D automatic (r = 0.943), followed by 3D manual (r = 0.919), and 2D DSA (r = 0.882) measurements. Measured median volumes were significantly different for 3D automatic and 2D DSA (p = 0.017). The highest rate of successful implantation (87.5%) was between 0.6 and 0.8 DAV ratio. Conclusion: Pre-procedural assessment of DAV ratios may increase WEB device implantation success. Our results suggest that volumetric measurements, especially using automated 3D volumes of the aneurysms, can assist in accurate WEB device size selection.
A Prospective Real-World Multi-Center Study to Evaluate Progression-Free and Overall Survival of Radiotherapy with Cetuximab and Platinum-Based Chemotherapy with Cetuximab in Locally Recurrent Head and Neck Cancer
Authors: Hecht Markus , Hahn Dennis , Wolber Philipp , Hautmann Matthias G. , Reichert Dietmar , Weniger Steffi , Belka Claus , Bergmann Tobias , Göhler Thomas , Welslau Manfred , Große-Thie Christina , Guntinas-Lichius Orlando , von der Grün Jens , Balermpas Panagiotis , Orlowski Katrin , Messinger Diethelm , Stenzel Karsten G. , Fietkau Rainer
Published: July 2021

Treatment options of locoregional recurrent head and neck squamous cell cancer (HNSCC) include both local strategies as surgery or re-radiotherapy and systemic therapy. In this prospective, multi-center, non-interventional study, patients were treated either with platinum-based chemotherapy and cetuximab (CT + Cet) or re-radiotherapy and cetuximab (RT + Cet). In the current analysis, progression-free survival (PFS) and overall survival (OS) were compared in patients with locoregional recurrence. Four hundred seventy patients were registered in 97 German centers. After exclusion of patients with distant metastases, a cohort of 192 patients was analyzed (129 CT + Cet, 63 RT + Cet). Radiotherapy was delivered as re-irradiation to 70% of the patients. The mean radiation dose was 51.8 Gy, whereas a radiation dose of ≥60 Gy was delivered in 33% of the patients. Chemotherapy mainly consisted of cisplatin/5-flurouracil (40%) or carboplatin/5-flurouracil (29%). The median PFS was 9.2 months in the RT + Cet group versus 5.1 months in the CT + Cet group (hazard ratio for disease progression or death, 0.40, 95% CI, 0.27–0.57, p < 0.0001). Median OS was 12.8 months in the RT + Cet group versus 7.9 months in the CT + Cet group (hazard ratio for death, 0.50, 95% CI, 0.33–0.75, p = 0.0008). In conclusion, radiotherapy combined with cetuximab improved survival compared to chemotherapy combined with cetuximab in locally recurrent HNSCC.
Intersection of Two Checkpoints: Could Inhibiting the DNA Damage Response Checkpoint Rescue Immune Checkpoint-Refractory Cancer?
Authors: Goff Peter H. , Bhakuni Rashmi , Pulliam Thomas , Lee Jung Hyun , Hall Evan T. , Nghiem Paul
Published: July 2021

Metastatic cancers resistant to immunotherapy require novel management strategies. DNA damage response (DDR) proteins, including ATR (ataxia telangiectasia and Rad3-related), ATM (ataxia telangiectasia mutated) and DNA-PK (DNA-dependent protein kinase), have been promising therapeutic targets for decades. Specific, potent DDR inhibitors (DDRi) recently entered clinical trials. Surprisingly, preclinical studies have now indicated that DDRi may stimulate anti-tumor immunity to augment immunotherapy. The mechanisms governing how DDRi could promote anti-tumor immunity are not well understood; however, early evidence suggests that they can potentiate immunogenic cell death to recruit and activate antigen-presenting cells to prime an adaptive immune response. Merkel cell carcinoma (MCC) is well suited to test these concepts. It is inherently immunogenic as ~50% of patients with advanced MCC persistently benefit from immunotherapy, making MCC one of the most responsive solid tumors. As is typical of neuroendocrine cancers, dysfunction of p53 and Rb with upregulation of Myc leads to the very rapid growth of MCC. This suggests high replication stress and susceptibility to DDRi and DNA-damaging agents. Indeed, MCC tumors are particularly radiosensitive. Given its inherent immunogenicity, cell cycle checkpoint deficiencies and sensitivity to DNA damage, MCC may be ideal for testing whether targeting the intersection of the DDR checkpoint and the immune checkpoint could help patients with immunotherapy-refractory cancers.
Phosphorylation of OGFOD1 by Cell Cycle-Dependent Kinase 7/9 Enhances the Transcriptional Activity of RNA Polymerase II in Breast Cancer Cells
Authors: Lee Han-Teo , Lee Il-Hwan , Kim Jae-Hwan , Lee Sangho , Kwak Sojung , Suh Min-Young , Hwang In-Young , Kang Bu-Gyeong , Cha Sun-Shin , Lee Byung-Il , Lee Sang-Eun , Choi Jinmi , Roe Jae-Seok , Cho Eun-Jung , Youn Hong-Duk
Published: July 2021

2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1 (OGFOD1) expression is upregulated in a variety of cancers and has been related to poor prognosis. However, despite this significance to cancer progression, the precise oncogenic mechanism of OGFOD1 is not understood. We demonstrated that OGFOD1 plays a role in enhancing the transcriptional activity of RNA polymerase II in breast cancer cells. OGFOD1 directly binds to the C-terminal domain of RNA polymerase II to alter phosphorylation status. The elimination of OGFOD1 resulted in decreased tumor development. Additionally, cell cycle-dependent kinase 7 and cell cycle-dependent kinase 9, critical enzymes for activating RNA polymerase II, phosphorylated serine 256 of OGFOD1, whereas a non-phosphorylated mutant OGFOD1 failed to enhance transcriptional activation and tumor growth. Consequently, OGFOD1 helps promote tumor growth by enhancing RNA polymerase II, whereas simultaneous phosphorylation of OGFOD1 by CDK enzymes is essential in stimulating RNA polymerase II-mediated transcription both in vitro and in vivo, and expression of target genes.
High-Trough Plasma Concentration of Afatinib Is Associated with Dose Reduction
Authors: Takahashi Takayuki , Terazono Hideyuki , Suetsugu Takayuki , Sugawara Hideki , Arima Junko , Nitta Mina , Tanabe Toru , Okutsu Kayu , Ikeda Ryuji , Mizuno Keiko , Inoue Hiromasa , Takeda Yasuo
Published: July 2021

Afatinib is used to treat non-small-cell lung cancer (NSCLC) harboring epidermal growth factor receptor (EGFR) mutation as a second-generation EGFR-tyrosine kinase inhibitor (TKI). Early prediction of adverse effects based on the pharmacokinetics of afatinib enables support for quality of life (QOL) in patients with no change in efficacy. We examined the pharmacokinetic relationship between trough plasma concentration and adverse effects and evaluated the utility of measuring the trough plasma concentration of afatinib as the first EGFR-TKI treatment for NSCLC in a prospective multicenter study. Twenty-four patients treated with afatinib were enrolled in this study. All blood samples were collected at the trough point, and plasma concentrations were measured using high-performance liquid chromatography–tandem mass spectrometry. Logistic regression analysis for the dose reduction of afatinib was performed, and the receiver operating characteristic (ROC) curve was plotted. Although all patients started afatinib at 40 mg/day, plasma concentrations were variable, and mean and median trough plasma concentrations were 32.9 ng/mL and 32.5 ng/mL in this study, respectively. Minimum and maximum trough plasma concentrations were 10.4 ng/mL and 72.7 ng/mL, respectively. This variability was speculated to involve personal parameters such as laboratory data. However, no patient characteristics or laboratory data examined correlated with the trough plasma concentration of afatinib, except albumin. Albumin showed a weak correlation with plasma concentration (r = 0.60, p = 0.009). The trough plasma concentration of afatinib was significantly associated with the dose reduction of afatinib (p = 0.047). The area under the ROC curve (AUC) for the trough plasma concentration of afatinib was 0.81. The cut-off value was 21.4 ng/mL. The sensitivity and specificity of the cut-off as a risk factor were 0.80 and 0.75. In summary, the trough plasma concentration of afatinib was associated with continued or reduced dosage because of the onset of several adverse effects, and a threshold was seen. Adverse effects not only lower QOL but also hinder continued treatment. Measuring plasma concentrations of afatinib appears valuable to predict adverse effects and continue effective therapy.
Synthetic Routes to Arylsulfonyl Fluorides
Authors: Chelagha Aida , Louvel Dan , Taponard Alexis , Berthelon Rodolphe , Tlili Anis
Published: July 2021

The goal of this mini-review is to shed the light on the existing methodologies to access arylsulfonyl fluorides. Today, a plethora of methods making use of a different pool of starting materials and in the presence of catalyst or under catalyst free conditions are disclosed in the literature.
Salivary Gland Tissue Engineering Approaches: State of the Art and Future Directions
Authors: Piraino Lindsay R. , Benoit Danielle S. W. , DeLouise Lisa A.
Published: July 2021

Salivary gland regeneration is important for developing treatments for radiation-induced xerostomia, Sjögren’s syndrome, and other conditions that cause dry mouth. Culture conditions adopted from tissue engineering strategies have been used to recapitulate gland structure and function to study and regenerate the salivary glands. The purpose of this review is to highlight current trends in the field, with an emphasis on soluble factors that have been shown to improve secretory function in vitro. A PubMed search was conducted to identify articles published in the last 10 years and articles were evaluated to identify the most promising approaches and areas for further research. Results showed increasing use of extracellular matrix mimetics, such as Matrigel®, collagen, and a variety of functionalized polymers. Soluble factors that provide supportive cues, including fibroblast growth factors (FGFs) and neurotrophic factors, as well as chemical inhibitors of Rho-associated kinase (ROCK), epidermal growth factor receptor (EGFR), and transforming growth factor β receptor (TGFβR) have shown increases in important markers including aquaporin 5 (Aqp5); muscle, intestine, and stomach expression 1 (Mist1); and keratin (K5). However, recapitulation of tissue function at in vivo levels is still elusive. A focus on identification of soluble factors, cells, and/or matrix cues tested in combination may further increase the maintenance of salivary gland secretory function in vitro. These approaches may also be amenable for translation in vivo to support successful regeneration of dysfunctional glands.
Father Presence, Father Engagement, and Specific Health and Developmental Outcomes of Mongolian Pre-School Children
Authors: Pablo Lesley A. , Erkhembayar Ryenchindorj , Davison Colleen M.
Published: July 2021

This study explored father involvement as a social determinant of child health within the context of macro-environmental changes in Mongolia. Using data for children aged 3–4 from UNICEF’s Multiple Indicator Cluster Surveys, this cross-sectional analysis examined the association between father presence and engagement with child health and educational outcomes. Multivariate regression modeling was employed to identify associations between father presence, engagement, and child outcomes including fever, respiratory illness, diarrhea and preschool attendance. In unadjusted analyses, father engagement was associated with higher odds of preschool attendance (Odds Ratio (OR) = 1.12; 95% Confidence Interval (CI) 1.04–1.20) but not with child illness (OR = 1.04; 95% CI 0.95–1.14). Father engagement was no longer associated with preschool attendance after controlling for potentially confounding variables (ORadj = 0.95; 95% CI 0.88–1.03). Unadjusted and adjusted analyses showed that father presence was not associated with acute illness or preschool attendance. Results also suggest that a larger proportion of children were engaged in activities by their mother compared to their father or other adults. Data indicate that father presence and engagement were not associated with child illness or preschool attendance. Factors such as maternal education, household wealth, and region of residence are stronger predictors of preschool attendance and should continue to be considered for promoting child health and development in Mongolia.