bluuebunny/crossref_metadata_2025 huggingface dataset:
TECNOLOGIA LEVE DE ENFERMAGEM COMO ALIADA NA CAMPANHA DE VACINAÇÃO COVID-19: RELATO DE EXPERIÊNCIA
Authors: Ferrony Rivas Claudia Maria , Cassol Camera Bruno , Oliveira dos Santos Naiana , Siepmann Soccol Keity Laís
Published: July 2022

Frente à pandemia da doença ocasionada pelo COVID-19, muitas foram as adaptações sociais no intuito de prevenção e proteção ao contágio.  O isolamento social trouxe importantes impactos na rotina da população, de forma especialmente rigorosa para pessoas idosas, já que em decorrência de fatores imunológicos e doenças de base, tem maior propensão a desenvolver a doença de forma grave. A privação social impulsiona o aparecimento de doenças mentais, ou mesmo pode agravar aquelas já existentes. Sob a luz do pensamento da complexidade e imbuídos de estratégias como a escuta e o acolhimento, esse artigo tem como objetivo relatar a experiência de um estudante do curso de graduação em enfermagem e a utilização da tecnologia leve de enfermagem nas campanhas de vacinação contra o COVID-19, durante o período de abril a agosto de 2021, que empregou a tecnologia leve de enfermagem como meio de acolhimento, escuta e promoção de vínculo com os usuários, afim de minimizar os impactos do isolamento social. As ações de vacinação, humanizadas pela tecnologia leve, e sob o pensamento da complexidade, mostram-se inovadoras e inclusivas, renovando a relação entre usuários e profissionais/estudantes da saúde.
ПОНЯТТЯ КРОСКУЛЬТУРНОЇ КОМУНІКАЦІЇ ВІЙСЬКОВОСЛУЖБОВЦІВ ЗБРОЙНИХ СИЛ УКРАЇНИ ПІД ЧАС ПІДГОТОВКИ ДЛЯ УЧАСТІ У МІЖНАРОДНИХ ОПЕРАЦІЯХ З ПІДТРИМАННЯ МИРУ І БЕЗПЕКИ
Authors: Красота-Мороз Ганна
Published: July 2022

Статья посвящена исследованию сущности и определению кросскультурной компетентности и коммуникации. Определены основные компоненты кросскультурной компетентности и методы обучения кросс-культурному взаимодействию. Проанализированы источники кросскультурной коммуникации в руководящих документах Министерства обороны Украины. Доказана необходимость дальнейшего исследования кросскультурного взаимодействия с дальнейшим его внедрением в курс подготовки военнослужащих, которые будут вовлечены в международные операции по поддержанию мира и безопасности.
ТОЧНІСТЬ ОБЧИСЛЕННЯ ОПТИМАЛЬНОЇ МАСИ ТІЛА СТУДЕНТОК ЗА РІВНЯННЯМ РЕГРЕСІЇ
Authors: Михайлов Володимир , Коростильова Юлія , Михайлов Віталій
Published: July 2022

Мета: розробка методики оцінювання якості рівняння регресії, за яким одержали оптимальну масу тіла студенток. Методи: теоретичний аналіз та узагальнення; педагогічний експеримент; антропометрія; метод найменших квадратів; регресійний аналіз. Матеріал: На основі математичного моделювання визначили точність корекції оптимальної маси тіла студенток (n = 116) з урахуванням їхньої конституції, віку і зросту. Результати: з’ясовано, що якість поправок оптимальної маси тіла студенток можна встановити відносно опорних формул за коефіцієнтом детермінації і середньою похибкою апроксимації у відсотках. Висновки: поправки на конституцію, вік і зріст студенток мають максимально вдалий підбір (D = 100 %) і забезпечують корекцію оптимального значення маси тіла з високою точністю (Ā = 0 %).
ПОЛІТИКА НАТО У СФЕРІ ВОЄННОЇ НАУКИ: ОРГАНІЗАЦІЯ НАУКОВОЇ І НАУКОВО-ТЕХНІЧНОЇ ДІЯЛЬНОСТІ, ПРИНЦИПИ ТА ПІДХОДИ
Authors: Полторак Микола , Горбачевський Сергій , Красота-Мороз Ганна , Мацьовитий Віктор , Плохута Ігор
Published: July 2022

У статті розглянуто проблематику щодо сутності політики НАТО, стосовно організації наукової і науково-технічної діяльності (ННТД), перспектив законодавства та нормативного регулювання щодо стану організації наукових досліджень у сфері воєнної науки, а також стисло окреслені:

бачення Командування об’єднаних збройних сил НАТО щодо основних питань з організації ННТД, використання різновиду моделей організації та проведення досліджень за напрямом воєнної науки, питань контролю за якістю наукової продукції, її  комплексного оцінювання та  ступеню  закритості наукової інформації;

деякі аспекти щодо особливостей функціонування систем управління суб’єктами ННТД, основні  принципи та підходи до створення і розвитку національних систем наукових досліджень у сфері воєнної науки.
АКТУАЛЬНІ ПРОБЛЕМИ ТРАНСФОРМАЦІЇ СТАНУ ТА ЯКОСТІ СИСТЕМИ ВИЩОЇ ВІЙСЬКОВОЇ ОСВІТИ
Authors: Приходько Юрій
Published: July 2022

В статті розглядається проблема трансформації стану та якості системи вищої військової освіти. Показується актуальність дослідження даної проблеми, що зумовлена політикою держави з реформування сектору безпеки та оборони, освіти, зміцнення потужності Збройних Сил України в умовах війни Російської Федерації проти України. Визначаються світові сучасні особливості та тенденції розвитку цивільних і військових освітніх систем, суперечності, притаманні функціонуванню системи військової освіти, загрози розвитку системі військової освіти. Обгрунтовуються актуальні проблеми трансформації вищої військової освіти, підготовки військових фахівців у вищих військових навчальних закладах як важливих складових сектору безпеки і оборони держави та основні напрями їх вирішення.
ОСНОВНІ ЕТАПИ СИСТЕМНОГО ПІДХОДУ ДО НАВЧАННЯ У ВИЩОМУ ВІЙСЬКОВОМУ НАВЧАЛЬНОМУ ЗАКЛАДІ
Authors: Рахманов Віталій , Тимчук Лариса
Published: July 2022

У статті розглядаються основні етапи системного підходу до навчання у вищому військовому навчальному закладі. У центрі уваги перебуває системність, яка за результатами навчання передбачає: критичне, раціональне мислення та вміння приймати оптимальні рішення в умовах бойових дій. Військова освіта ґрунтується на навчальних цілях, які можуть сформульовані з використанням системного підходу країн членів НАТО до навчання. В такий спосіб офіцерів готують до труднощів, які пов’язані з діяльністю у багатонаціональному, взаємосумісному середовищі НАТО.

У процесі навчання очікується, що офіцерам будуть надаватимуть такі знання, які пропорційні рівню виконання посадових обов’язків за посадою та відповідно до військового звання, що має бути основним критерієм оцінки досягнення результату системного підходу до військової освіти, оцінки якості навчальної діяльності і професійної підготовки майбутніх фахівців, а також оцінки ВВНЗ, які мають зосереджувати освітній процес на отримання слухачами професійних компетентностей у сфері професійної діяльності. Це вимагає застосування нових підходів до формування змісту військової освіти, яка має орієнтуватись та визначатись конкретними завданнями, що вирішуються у військах: планування й проведення спільних операцій (загальновійськових, спеціальних тощо), досягнення високого рівня операційної сумісності, забезпечення міжвідомчої взаємодії та координації планування і проведення операцій на рівні оперативно-тактичних угруповань тощо.

З точки зору виокремлення специфіки військової професії, важливо акцентувати увагу на тому, що офіцер має справу з невизначеністю, що у поєднанні з відповідальністю за прийняття службових рішень та невід’ємною особливістю професії військового формує вимоги до змісту, якості та рівня військової освіти: реалізація наявного потенціалу особистості шляхом надання широкого комплексу знань у різних сфер життєдіяльності людини і суспільства. Потреби Збройних Сил України у висококваліфікованих військових професіоналах визначають пріоритети військової освіти, професійної підготовки, перепідготовки та підвищення кваліфікації. Система військової освіти на сучасному етапі виступає водночас як об’єктом, так і суб’єктом реформування всього комплексу сил оборони. Саме висока якість підготовки військових фахівців є важливою умовою своєчасності, повноти та ефективності виконання заходів оборонної реформи в державі та є основою у формуванні політики професійної військової освіти держави.
ДІДЖИТАЛІЗАЦІЯ ФІЗИЧНОГО ВИХОВАННЯ: БАР’ЄРИ ТА МОЖЛИВОСТІ
Authors: Саїнчук Микола , Вербин Назарій , Саїнчук Анна
Published: July 2022

Розглянуто потенціал діджиталізації у фізичному вихованні та представлено форми цифрових технологій, які активно використовують зацікавлені викладачі фізичного виховання різних країн світу в своїй освітній діяльності. Встановлено, що пандемія COVID-19 прискорила імплементацію діджитал технологій у фізичне виховання. Окреслено переваги та недоліки онлайн фізичного виховання. Ознайомлено із двома можливими бар’єрами (правовим і гуманітарним) на шляху становлення діджиталізованого фізичного виховання.
Accounting for Non-Uniform Ambient Lighting in Ray Traced Ambient Occlusion
Authors: Fridvalszky András , Tóth Balázs
Published: July 2022

Ambient occlusion is a popular method to enhance the visuals of real-time applications. The generally accepted equation assumes that incoming ambient lighting is uniform in the enclosing hemisphere around the surface point. This assumption does not always hold and can cause artifacts. With screen-space techniques the artifact may not be present, because of the lack of information, but it becomes visible with more accurate methods. This is especially true for ray tracing based implementations which are widely used to create the ground truth image to measure other techniques. With recent advancements of the graphics hardware, they also found their way into real-time applications where these shortcomings were neither discussed nor addressed. In this paper we analyze the problem and demonstrate its presence in popular game engines. We also propose a ray tracing based extension to eliminate these artifact in a physically plausible way.
Raznolikost samoniklih korisnih biljaka travnjaka Ćićarije (Hrvatska) - hrana za ljude i životinje
Authors: Vitasović-Kosić Ivana , Britvec Mihaela
Published: July 2022

Samonikla flora i fauna nisu u potpunosti iskorištene u ljudskoj prehrani. U ovom radu prikazani su rezultati terenskih botaničkih istraživanja uglavnom zeljastih vrsta i podvrsta suhih mediteranskih travnjaka i šumskih rubova područja Ćićarije. Tijekom više vegetacijskih sezona od proljeća do jeseni floristički je istraživano područje Natura 2000 zaštićenih staništa (habitat 62A0). U cilju povećanja mogućnosti proizvodnje hrane i raznolikosti poljoprivrednih proizvoda, ovdje je izdvojeno i opisano 106 svojti. Od 32 vrste koje se koriste kao hrana (npr. Artium lappa, Scorzonera hispanica, Capsella bursa-pastoris, Diplotaxis tenuifolia, Rumex acetosa i dr.), najviše je samoniklog povrća (19 svojti), 9 vrsta se koristi kao samoniklo voće i 4 vrste kao baza za izradu pića. Od 46 vrsta koje se koriste kao hrana za životinje, većina ih spada u porodicu trava (Poaceae), među njima nalazimo 3 vrste koje se koriste kao samoniklo povrće (Portulaca oleracea, Trifolium incarnatum i T. aureum) i 3 kao arome (Elymus repens, Melilotus officinalis i Trifolium pratense). Također među njima nalazimo 8 medonosnih biljaka (npr. Marrubium vulgare, Melilotus officinalis). U kategoriji materijala odnosno tvari nalazimo biljke poput Hyssopus officinalis, Juniperus communis, Salvia officinalis i dr. Povećanje proizvodnje hrane od samoniklih biljaka koje nas okružuju, kao i povećanje prerade u određene proizvode (npr. začina i dodataka hrani) je moguće, pri tome imajući u vidu potrebu zaštite okoliša i aktualne klimatske promjene.
The need in medical rehabilitation for the adult population of the Khabarovsk Krai
Authors:   Postgraduate Institute for Public Health Workers under Health Ministry of Khabarovsk Krai, Ivasyuk G.V. , Skovycheva L.D. ,   Postgraduate Institute for Public Health Workers under Health Ministry of Khabarovsk Krai
Published: July 2022

The article provides the data on the state of the medical rehabilitation system in the Khabarovsk Krai based on statistic reports data for 2019 (selected as the last profile year, since the healthcare system was re-profiled due to the Covid-19 epidemic after 2019). Proposals have been suggested to improve accessibility and quality, for the further development of medical care for medical rehabilitation to the population of the Khabarovsk Krai, in order to implement the initiative of the Government of the Russian Federation in the Khabarovsk Krai "The optimal medical rehabilitation for health recovery"
Optimization of intra-laboratory quality control methods
Authors:   The Regional Clinical Hospital named after Professor O.V. Vladimirtsev, Amelina T.K. , Mizko T.V. ,   The Regional Clinical Hospital named after Professor O.V. Vladimirtsev             ladimirtsev, Skurlatova O.V. ,   The Regional Clinical Hospital named after Professor O.V. Vladimirtsev, Chemezova N.M. ,   The Regional Clinical Hospital named after Professor O.V. Vladimirtsev
Published: July 2022

The article outlines and analyzes the experience of optimizing intra-laboratory quality control of the analytical stage of research in the clinical and diagnostic laboratory. Emphasized is the advantage of the use of automated electronic cards in comparison with processing indicators manually by reducing time and increasing the accuracy of laboratory data
Anns gach gnìomh a nì duine ’s mór urram nan gàidheal: Donnchadh bàn Mac an t-Saoir as the Voice of the Gael’s Military Masculinity in the Eighteenth Century
Authors: Hulle Liesbeth Van
Published: July 2022

The eighteenth century witnessed the changed perception of the Highland male as he evolved from an unruly member of a warrior society to the ideal soldier, participating in the military activities of the British empire. This article explores firstly, how this transformation came about, from the celebration of the warrior society the Gàidhealtachd appeared to be, to the personal identification with the Gael's martial self. Secondly, this article establishes how and why the ordinary Highland man embraced this military identity. As the voice of the eighteenth-century Gael is rather difficult to trace, the life and oeuvre of Donnchadh Bàn Mac an t-Saoir (Duncan Bàn MacIntyre) is used to represent his fellow Highlanders. Donnchadh Bàn spent his entire adult life carrying arms and was a life-long proponent of the military masculinity the Gael displayed. Especially his enlistment in the Breadalbane Fencibles provides a unique insight. When his fervour for the Gael's warrior masculinity is checked against the many letters from the tenants on the Breadalbane estate, it is not a picture of an innately warrior masculinity that emerges, but one of a man choosing a temporary military path to hunt down economic security and independence.
The Earl of Buchan's Second Summer: Dryburgh (1785–1829)
Authors: Wood John D.
Published: July 2022

After over a century of relative neglect, the activities of David Steuart Erskine, the eleventh Earl of Buchan, on his Dryburgh estate between 1785 and 1829 have come under increasing academic scrutiny. The Earl’s retirement to Dryburgh has been seen less as a retreat from his high public profile as the founder of the Society of Antiquaries and more as a continuation both of his antiquarian and political interests. Indeed, landscape features such as the Temple of the Muses to the poet James Thomson and especially the giant statue to William Wallace have been viewed recently as part of a highly political nationalist-historic romantic landscape. While confirming the essentially political nature of these monuments, this article will explore an alternative whig and unionist reading of them. At the same time, although highlighting the continuity of Buchan’s antiquarian agenda, it will attempt to show how this gradually merged with a wide range of the Earl’s more personal enthusiasms such as Horace Walpole’s Strawberry Hill, classical antiquity and the beaux arts of London’s Royal Academy to form a distinctive second summer to his career.
‘No Longer the Preserve of Townsfolk’: Female Electrical Mediators in Northern Scotland and the Craibstone School of Rural Domestic Economy, 1923–1939
Authors: Peters Eleanor
Published: July 2022

This article examines the role of rural women as electrical mediators in the north of Scotland during the interwar years, focusing on the activities of female representatives of the Craibstone School of Rural Domestic Economy (CSRDE). During this period, female teachers and students working for the CSRDE adopted and promoted electrical appliances, fostering enthusiasm for electrification among women living in the north of Scotland. Notably, at the Highland Show in 1935, students from the CSRDE entertained thousands of visitors by demonstrating appliances from an ‘all-electric’ kitchen, stimulating a trend for domestic electrical displays at future Highland Shows. Significantly, this article adds to the historiography of the electrification of Scotland, which has hitherto been dominated by male actors. Beyond Scotland, scholars have argued that affluent urban women framed electrification as an opportunity for liberation from the domestic sphere. However, using the records from the CSRDE, I recover the overlooked attitudes of rural women and show that they did not conceive of electrical appliances in the same light. This article provides an insight into the vital, yet forgotten, mediating role that rural women performed between the electrical industry and consumers.
Decomposition of Natural Numbers from Prime Objects
Authors: Sebata Junya
Published: July 2022

We decompose natural numbers from structure which prime numbers have, as its starting point. With the decomposition, we can find a general law by categorization, which is in a power set and also in structure which prime numbers have, and we know that it limits the framework of structure about product and sum of natural numbers. In other words, $\sum_{k=1}^{n} \phi (k) \times [\frac{n}{k}] = \frac{n(n+1)}{2}$ holds, and it is equivalent to a basic formula of sum of divisors $\sum_{k|n} \phi (k) = n$.
VS-Neuron Cell
Authors: Shiwhare Vichar
Published: July 2022

Humans and some animals have logical thinking and feelings, but today, AI machines don’t. This Artificial Neuron named VS-Neuron has the capability of such deeds. Neural Network of VS-Neuron can lead active brain.Which have the ability as the human brain has.
Validation of a Patient-Specific Musculoskeletal Model for Lumbar Load Estimation Generated by an Automated Pipeline From Whole Body CT
Authors: Lerchl Tanja , El Husseini Malek , Bayat Amirhossein , Sekuboyina Anjany , Hermann Luis , Nispel Kati , Baum Thomas , Löffler Maximilian T. , Senner Veit , Kirschke Jan S.
Published: July 2022

Background: Chronic back pain is a major health problem worldwide. Although its causes can be diverse, biomechanical factors leading to spinal degeneration are considered a central issue. Numerical biomechanical models can identify critical factors and, thus, help predict impending spinal degeneration. However, spinal biomechanics are subject to significant interindividual variations. Therefore, in order to achieve meaningful findings on potential pathologies, predictive models have to take into account individual characteristics. To make these highly individualized models suitable for systematic studies on spinal biomechanics and clinical practice, the automation of data processing and modeling itself is inevitable. The purpose of this study was to validate an automatically generated patient-specific musculoskeletal model of the spine simulating static loading tasks.

Methods: CT imaging data from two patients with non-degenerative spines were processed using an automated deep learning-based segmentation pipeline. In a semi-automated process with minimal user interaction, we generated patient-specific musculoskeletal models and simulated various static loading tasks. To validate the model, calculated vertebral loadings of the lumbar spine and muscle forces were compared with in vivo data from the literature. Finally, results from both models were compared to assess the potential of our process for interindividual analysis.

Results: Calculated vertebral loads and muscle activation overall stood in close correlation with data from the literature. Compression forces normalized to upright standing deviated by a maximum of 16% for flexion and 33% for lifting tasks. Interindividual comparison of compression, as well as lateral and anterior–posterior shear forces, could be linked plausibly to individual spinal alignment and bodyweight.

Conclusion: We developed a method to generate patient-specific musculoskeletal models of the lumbar spine. The models were able to calculate loads of the lumbar spine for static activities with respect to individual biomechanical properties, such as spinal alignment, bodyweight distribution, and ligament and muscle insertion points. The process is automated to a large extent, which makes it suitable for systematic investigation of spinal biomechanics in large datasets.
Sensor-Based Nerve Compression Measurement: A Scoping Review of Current Concepts and a Preclinical Evaluation of Commercial Microsensors
Authors: Daeschler Simeon C. , Wienbruch Rebecca , Bursacovschi Catalina , Zimmermann Kim Sophie , Nemariam Selam Bekure , Harhaus Leila , Kneser Ulrich , Dehé Alfons , Bittner Achim
Published: July 2022

Background: Chronic nerve compression is the most common indication for nerve surgery. However, the clinical diagnosis still relies on surrogate parameters since devices for direct nerve compression pressure measurement (DNCPM) are clinically unavailable yet.

Objectives: To review previous approaches to DNCPM and evaluate presently available microsensor systems for their feasibility and reliability in preclinical nerve compression models.

Methods: A scoping literature review was conducted in accordance with the PRISMA-ScR guidelines. A subsequent market research aimed at identifying commercially available sensor systems potentially suitable for DNCPM. Sensors were evaluated for feasibility and safety of perineural sensor positioning, tissue compatibility and measurement reliability in a synthetic nerve compression model and an ex-vivo chicken leg model.

Results: A scoping literature review identified 197 potentially eligible studies of which 65 were included in the analysis. Previous approaches to DNCPM predominantly used pressure sensing catheters designed for fluid- or intra-compartmental pressure measurement. A market research identified two piezoresistive sensor systems (IntraSense, SMi, United States; Mikro-Cath, Millar, United States) as potentially suitable for intraoperative DNCPM. In both preclinical models, the detected compression pressure differed significantly between sensors and systems showed substantial measurement variability with a median percent coefficient of variation between 15.5% and 32%. Sensor position was accountable for up to 99.1% of the variance.

Conclusion: Measurement variability caused by unreliable sensor positioning is a key limitation of presently available sensors when applied for nerve compression measurements. Redesigned systems with small, flat-shaped and longitudinally oriented sensors and dedicated introducers would facilitate sensor positioning and therefore may allow for reliable measurements.
Young Individuals Are More Stable and Stand More Upright When Using Rollator Assistance During Standing up and Sitting Down
Authors: Krafft F. C. , Herzog M. , Stein T. , Sloot L. H.
Published: July 2022

Four-wheeled walkers or rollators are often used to assist older individuals in maintaining an independent life by compensating for muscle weakness and reduced movement stability. However, limited biomechanical studies have been performed to understand how rollator support affects posture and stability, especially when standing up and sitting down. Therefore, this study examined how stability and posture change with varying levels of rollator support and on an unstable floor. The aim was to collect comprehensive baseline data during standing up and sitting down in young participants. In this study, 20 able-bodied, young participants stood up and sat down both 1) unassisted and assisted using a custom-made robot rollator simulator under 2) full support and 3) touch support. Unassisted and assisted performances were analyzed on normal and unstable floors using balance pads with a compliant surface under each foot. Using 3D motion capturing and two ground-embedded force plates, we compared assistive support and floor conditions for movement duration, the relative timing of seat-off, movement stability (center of pressure (COP) path length and sway area), and posture after standing up (lower body sagittal joint angles) using ANOVA analysis. The relative event of seat-off was earliest under full support compared to touch and unassisted conditions under normal but not under unstable floor conditions. The duration of standing up and sitting down did not differ between support conditions on normal or unstable floors. COP path length and sway area during both standing up and sitting down were lowest under full support regardless of both floor conditions. Hip and knee joints were least flexed under full support, with no differences between touch and unassisted in both floor conditions. Hence, full rollator support led to increased movement stability, while not slowing down the movement, during both standing up and sitting down. During standing up, the full support led to an earlier seat-off and a more upright standing posture when reaching a stable stance. These results indicate that rollator support when handles are correctly aligned does not lead to the detrimental movement alterations of increased forward-leaning. Future research aims to verify these findings in older persons with stability and muscle weakness deficiencies.
Tension Estimation Method for Cable With Damper Using Natural Frequencies and Two-Point Mode Shapes With Uncertain Modal Order
Authors: Furukawa Aiko , Suzuki Syuya , Kobayashi Ryosuke
Published: July 2022

In the maintenance of cable structures, such as cable-stayed bridges, cable safety is assessed based on the cable tension. In Japan, the cable tension is generally estimated from the cable’s natural frequencies using the higher-order vibration method. In recent years, dampers have been installed onto cables to suppress aerodynamic vibrations. Because the damper changes the cable’s natural frequencies, the damper is removed to measure the natural frequencies and estimate the cable tension without a damper, and the damper is then reinstalled. To avoid damper removal and reinstallation, the authors previously proposed Method 2F for estimating the tension of a cable with a damper from the natural frequencies without removing the damper. Because the tension estimation error of the higher-order vibration method for a cable without a damper has been reported as 5%, the authors set the target tension estimation error within 5%. However, the tension estimation error of Method 2F exceeded 5% in the experimental verification. Furthermore, although Method 2F estimates the tension and bending stiffness of the cable and the damper parameters simultaneously from the natural frequencies, the accuracy of the bending stiffness and damper parameters is unsatisfactory. In this paper, the new Method 2FM is proposed to estimate the tension and bending stiffness of the cable and damper parameters using the natural frequencies and two-point mode shapes. With the addition of mode shapes, Method 2FM attempts to improve the accuracy of estimating the tension and other parameters. The validity of Method 2FM was confirmed by numerical simulations and experiments. The numerical verification confirmed that Method 2FM is superior to Method 2F in estimating the cable tension and damper parameters. The experimental verification confirmed that the tension estimation accuracy of Method 2FM is higher than that of Method 2F, and the estimation error is lower than 5%. However, the damper parameters estimated by Method 2FM are different to the design values. The reason for this is the modeling error of the damper, as found by conducting an element test on the damper.
A Pex7 Deficient Mouse Series Correlates Biochemical and Neurobehavioral Markers to Genotype Severity—Implications for the Disease Spectrum of Rhizomelic Chondrodysplasia Punctata Type 1
Authors: Fallatah Wedad , Cui Wei , Di Pietro Erminia , Carter Grace T. , Pounder Brittany , Dorninger Fabian , Pifl Christian , Moser Ann B. , Berger Johannes , Braverman Nancy E.
Published: July 2022

Rhizomelic chondrodysplasia punctata type 1 (RCDP1) is a peroxisome biogenesis disorder caused by defects in PEX7 leading to impairment in plasmalogen (Pls) biosynthesis and phytanic acid (PA) oxidation. Pls deficiency is the main pathogenic factor that determines the severity of RCDP. Severe (classic) RCDP patients have negligible Pls levels, congenital cataracts, skeletal dysplasia, growth and neurodevelopmental deficits, and cerebral hypomyelination and cerebellar atrophy on brain MRI. Individuals with milder or nonclassic RCDP have higher Pls levels, better growth and cognitive outcomes. To better understand the pathophysiology of RCDP disorders, we generated an allelic series of Pex7 mice either homozygous for the hypomorphic allele, compound heterozygous for the hypomorphic and null alleles or homozygous for the null allele. Pex7 transcript and protein were almost undetectable in the hypomorphic model, and negligible in the compound heterozygous and null mice. Pex7 deficient mice showed a graded reduction in Pls and increases in C26:0-LPC and PA in plasma and brain according to genotype. Neuropathological evaluation showed significant loss of cerebellar Purkinje cells over time and a decrease in brain myelin basic protein (MBP) content in Pex7 deficient models, with more severe effects correlating with Pex7 genotype. All Pex7 deficient mice exhibited a hyperactive behavior in the open field environment. Brain neurotransmitters analysis of Pex7 deficient mice showed a significant reduction in levels of dopamine, norepinephrine, serotonin and GABA. Also, a significant correlation was found between brain neurotransmitter levels, the hyperactivity phenotype, Pls level and the severity of Pex7 genotype. In conclusion, our study showed evidence of a genotype-phenotype correlation between the severity of Pex7 deficiency and several clinical and neurobiochemical phenotypes in RCDP1 mouse models. We propose that PA accumulation may underlie the cerebellar atrophy seen in older RCDP1 patients, as even relatively low tissue levels were strongly associated with Purkinje cells loss over time in the murine models. Also, our data demonstrate the interrelation between Pls, brain neurotransmitter deficiencies and the neurobehavioral phenotype, which could be further used as a valuable clinical endpoint for therapeutic interventions. Finally, these models show that incremental increases in Pex7 levels result in dramatic improvements in phenotype.
Shared Transcriptional Machinery at Homologous Alleles Leads to Reduced Transcription in Early Drosophila Embryos
Authors: Deng Hao , Lim Bomyi
Published: July 2022

The mechanism by which transcriptional machinery is recruited to enhancers and promoters to regulate gene expression is one of the most challenging and extensively studied questions in modern biology. We explored the possibility that interallelic interactions between two homologous alleles might affect gene regulation. Using an MS2- and PP7-based, allele-specific live imaging assay, we visualized de novo transcripts of a reporter gene in hemizygous and homozygous Drosophila embryos. Surprisingly, each homozygous allele produced fewer RNAs than the corresponding hemizygous allele, suggesting the possibility of allelic competition in homozygotes. However, the competition was not observed when the enhancer-promoter interaction was weakened by placing the reporter construct in a different chromosome location or by moving the enhancer further away from the promoter. Moreover, the reporter gene showed reduced transcriptional activity when a partial transcription unit (either an enhancer or reporter gene only) was in the homologous position. We propose that the transcriptional machinery that binds both the enhancer and promoter regions, such as RNA Pol II or preinitiation complexes, may be responsible for the allelic competition. We showed that the degree of allelic interference increased over developmental time as more Pol II was needed to activate zygotic genes. Such allelic competition was observed for an endogenous gene as well. Our study provides new insights into the role of 3D interallelic interactions in gene regulation.
The LIM Protein AJUBA is a Potential Oncogenic Target and Prognostic Marker in Human Cancer via Pan-Cancer Analysis
Authors: Song Na , Liu Jia , Zhang Ke , Yang Jie , Cui Kai , Miao Zhuang , Zhao Feiyue , Meng Hongjing , Chen Lu , Chen Chong , Li Yushan , Shao Minglong , Su Wei , Wang Haijun
Published: July 2022

Purpose: The LIM (Lin-11, Isl1, MEC-3) domain protein AJUBA is involved in multiple biological functions, and its aberrant expression is related to the occurrence and progression of various cancers. However, there are no analytical studies on AJUBA in pan-cancer.

Methods: We performed a comprehensive pan-cancer analysis and explored the potential oncogenic roles of AJUBA, including gene expression, genetic mutation, protein phosphorylation, clinical diagnostic biomarker, prognosis, and AJUBA-related immune infiltration based on The Cancer Genome Atlas and Genotype-Tissue Expression databases.

Results: The results revealed that the expression of AJUBA highly correlated with poor clinical outcomes in patients with different types of cancer. Meanwhile, AJUBA expression was positively correlated with cancer-associated fibroblasts in many human cancers, such as breast invasive carcinoma, colon adenocarcinoma, brain lower-grade glioma, lung adenocarcinoma (LUAD), and ovarian serous cystadenocarcinoma (OV). Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that AJUBA is mainly involved in protein serine/threonine kinase activity, cell–cell junction, covalent chromatin modification, and Hippo signaling pathway.

Conclusion: The pan-cancer study reveals the oncogenic roles of AJUBA and provides a comprehensive understanding of the molecular biological genetic information of AJUBA in various tumors.
Self-Assembly of Homo-Polyarylene Ether Into Reactive Matrix for Fabrication of Hybrid Functional Microparticles
Authors: Gong Du , Zhang Dawei , Zhang Xiaoying , He Xiaohong , Ji Yao , Jia Kun
Published: July 2022

Emulsion confinement self-assembly of block copolymer has witnessed increasing research interest in the recent decade, but the post-functionalization and application of the resultant polymeric micro/nano-particles are still in their infancy. In this work, a super-engineering polyarylene ether containing pendent nitrile and carboxyl (PAE-NC) has been synthesized and converted into polymeric microparticles for macromolecular enrichment via emulsion confinement self-assembly and subsequent surface modification. Moreover, the encapsulation capacity of PAE-NC was evaluated using hydrophobic fluorescent quantum dots (QD) as a functional probe. Particularly, we found that both the as-synthesized PAE-NC and its hydrolyzed derivatives could be converted into microparticles via emulsion confinement self-assembly. Furthermore, the co-self-assembly of red-emitting QD and PAE-NC enables the phase transfer of hydrophobic QD into hydrophilic luminescent microparticles with the persisted fluorescence emission. Based on these results, the current PAE-NC would be served as a versatile and robust matrix to fabricate advanced microparticles or microcapsules for various applications.
A Novel Tannic Acid-Based Carbon-Supported Cobalt Catalyst for Transfer Hydrogenation of Biomass Derived Ethyl Levulinate
Authors: Wang Meng , Yao Xuefeng , Chen Yuxin , Lin Baodong , Li Na , Zhi Keduan , Liu Quansheng , Zhou Huacong
Published: July 2022

The catalytic conversion of ethyl levulinate (EL) to γ-valerolactone (GVL) is an important intermediate reaction in the conversion and utilization of biomass resources. The development of novel and efficient catalysts is significantly important for this reaction. In this work, using the biomass-derived tannic acid as carbon precursor and the transition metal cobalt as active component, a novel tannic acid carbon supported cobalt catalyst (Co/TAC) was prepared by pyrolysis and subsequent hydrazine hydrate reduction method. The hydrogenation of EL and other carbonyl compounds by hydrogen transfer reaction was used to evaluate the performance of the catalysts. The effects of different preparation and reaction conditions on the performance of the catalysts were investigated, and the structures of the prepared catalysts were characterized in detail. The results showed that the carbonization temperature of the support had a significant effect on the activity of the catalyst for the reaction. Under the optimized conditions, the Co/TAC-900 catalyst obtained the highest GVL yield of 91.3% under relatively mild reaction conditions. Furthermore, the prepared catalyst also showed high efficiency for the hydrogenation of various ketone compounds with different structures. This work provides a new reference for the construction of the catalysts during the conversion of biomass and a potential pathway for the high-value utilization of tannin resource.
Neutralizing Activity Against SARS-CoV-2 Delta and Omicron Variants Following a Third BNT162b2 Booster Dose According to Three Homologous or Heterologous COVID-19 Vaccination Schedules
Authors: Choi Ju-yeon , Lee Young Jae , Ko Jae-Hoon , Kim Su-Hwan , Kim Hye-Jin , Lee Hye Won , Jeong Hyeonji , Kim Tae-Yong , Jang Yeong Gyeong , Hong Hyo-jeong , Kim Min-Seong , Lee Sang Eun , Kim Yong Guan , Chung Eun Joo , Lim Heeji , Jang Sundong , Kim Kwangwook , Kim Sung Soon , Ahn Jin Young , Choi Jun Yong , Kim Yong Chan , Park Yoon Soo , Peck Kyong Ran , Kim Byoungguk
Published: July 2022

With the emergence and rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta and Omicron variants, escaping vaccine-induced immunity is a concern. Three vaccination schedules, homologous or heterologous, have been initially applied due to an insufficient supply of vaccines in Korea. We investigated neutralizing activities against Omicron and Delta variants in each schedule. Three schedules using three doses of the BNT162b2 (BNT) or the ChAdOx1 (ChAd) vaccines include ChAd-ChAd-BNT, ChAd-BNT-BNT, and BNT-BNT-BNT. Neutralizing activities were evaluated using plaque-reduction neutralization test (PRNT) against wild type (WT) SARS-CoV-2, Delta variant, and Omicron variant. A total of 170 sera from 75 participants were tested, and the baseline characteristics of participants were not significantly different between groups. After the 2nd vaccine dose, geometric mean titers of PRNT ND50 against WT, Delta, and Omicron were highest after ChAd-BNT vaccination (2,463, 1,097, and 107) followed by BNT-BNT (2,364, 674, and 38) and ChAd-ChAd (449, 163, and 25). After the 3rd dose of BNT, the increase of PRNT ND50 against WT, Delta, and Omicron was most robust in ChAd-ChAd-BNT (4,632, 988, and 260), while the BNT-BNT-BNT group showed the most augmented neutralizing activity against Delta and Omicron variants (2,315 and 628). ChAd-BNT-BNT showed a slight increase of PRNT ND50 against WT, Delta, and Omicron (2,757, 1,279, and 230) compared to the 2nd dose. The results suggest that a 3rd BNT booster dose induced strengthened neutralizing activity against Delta and Omicron variants. The waning of cross-reactive neutralizing antibodies after the 3rd dose and the need for additional boosting should be further investigated.
The Potential Anti-remodeling Effect of Paroxetine After Myocardial Infarction May Be Blunted by Beta-Blockers
Authors: Iborra-Egea Oriol , Aimo Alberto , Martini Nicola , Galvez-Monton Carolina , Burchielli Silvia , Panichella Giorgia , Passino Claudio , Emdin Michele , Bayes-Genis Antoni
Published: July 2022

Background

Left ventricular (LV) remodeling consists in maladaptive changes in cardiac geometry and function following an insult such as ST-segment elevation myocardial infarction (STEMI). Interventions able to prevent LV remodeling after a STEMI are expected to improve the outcome of this condition. Paroxetine has inhibitory effects on GRK2, also known as beta-adrenergic receptor kinase 1 (ADRBK1). This drug does not yield beneficial effects on LV remodeling in patients with STEMI and LV ejection fraction ≤ 45%.

Methods

We compared the molecular effects of paroxetine and drugs for neurohormonal antagonism (beta-blockers, angiotensin converting enzyme inhibitors/angiotensin receptor blockers, mineralocorticoid receptor antagonists), using a bioinformatic approach integrating transcriptomic data in a swine model of post-MI and available evidence from the literature and massive public databases.

Results

Among standard therapies for MI, beta-blockers are the only ones acting directly upon GKR2, but the mechanism of action overlaps with angiotensin-converting enzyme inhibitors/angiotensin receptor blockers with respect to the AT2R-mediated anti-hypertensive response. Moreover, beta-blockers could have anti-fibrotic and anti-inflammatory effects through the regulation of myocyte-specific enhancer factors, endothelins and chemokines.

Conclusion

The additive benefit of paroxetine on the background of the standard therapy for STEMI, which includes beta-blockers, is expected to be limited. Nonetheless, paroxetine becomes particularly interesting when a beta-blocker is contraindicated (for example, in hypotensive individuals) or poorly tolerated.
Tropical Cyclones Intensity Prediction in the Western North Pacific Using Gradient Boosted Regression Tree Model
Authors: Zhu Gangya , Li Qinglan , Zhao Wei , Lv Xinyan , Qian Chuanhai , Qian Qifeng
Published: July 2022

As an artificial intelligence method, machine learning (ML) has been widely used in prediction models of high-dimensional datasets. This study proposes an ML method, the Gradient Boosted Regression Tree (GBRT), to predict the intensity changes of tropical cyclones (TCs) in the Western North Pacific at 12-, 24-, 36-, 48-, 60-, and 72-h (hr) forecasting lead time and the model is optimized by the Bayesian Optimization algorithm. The model predictands are the TCs intensity changes at different forecasting lead times, obtained from the best track data of the Shanghai Typhoon Institute (STI) and the Joint Typhoon Warning Center (JTWC) from 2000 to 2019. The model predictors are the synoptic variables, climatological and persistent variables derived from the reanalysis data obtained from the National Centers for Environmental Prediction (NCEP), and the sea surface temperature (SST) data obtained from the National Oceanic and Atmospheric Administration (NOAA). The results show that the GBRT model can capture the TCs intensity changes well for the succeeding 12-h, 24-h, 36-h, and 72-h. Compared with the traditional multiple linear regression (MLR) model, the GBRT model has better performance in predicting TCs intensity changes. Compared with the MLR model, R2 of the GBRT model for TCs intensity forecast increases by an average of 8.47% and 4.45% for STI data and JTWC data. MAE (RMSE) drops by 26.24% (25.14%) and 10.51% (4.68%) for the two datasets, respectively. The potential future intensity change (POT), the intensity changes during the previous 12 h (Dvmax), Initial storm maximum wind speed (Vmax), SST, and the Sea-Land ratio are the most significant predictors for the GBRT model in predicting TCs intensity change over the Western North Pacific.
OSL Dating of Paleo-Shorelines at Dagze Co, Central Tibetan Plateau
Authors: Wu Yang , Chen Yiwei , Huang Long , Ding Ping , Cui Hao , Wei Gangjian
Published: July 2022

Well-preserved paleo-shoreline systems suggest lake shrinkage occurred in a vast region of the Tibetan Plateau, indicating dramatic paleo-environmental changes that linked closely to variations in Asian monsoon precipitation, glacial meltwater, and evaporation. The elevation and chronology of these shoreline features can be used to reconstruct lake level fluctuation history and regional paleo-climate. Here, we report optically stimulated luminescence (OSL) dating of 14 sediment samples from three of seven paleo-shorelines at south of Dagze Co, central Tibetan Plateau, using coarse-grained quartz and K-feldspar. Two fine grain silt samples were also measured. The results suggest the following: 1) The highest paleo-shoreline for Dagze Co has an elevation around 4,525 m (+55 m above modern lake). Its age is estimated to be 9.1 ± 0.5 to 9.6 ± 0.6 ka, suggesting a relative humid condition in the early Holocene. 2) After that, the lake level dropped ∼20 m and remained relatively stable (+35 m) from ∼8 to 5 ka, implying a dry condition with reduced runoff. Lake level further declined for ∼40 m since about 5 ka. 3) The general trend of shrinkage for Dagze Co broadly matches studies from adjacent lakes, suggesting a common pattern of lake’s evolution on the south/central plateau in the late Quaternary. The long-term decreasing trend of Asian monsoon precipitation should be the main reason.
Comparative Transcriptome Analyses of Geriatric Rats Associate Age-Related Erectile Dysfunction With a lncRNA-miRNA-mRNA Regulatory Network
Authors: Zhou Xuan , Cong Rong , Yao Liangyu , Zhou Xiang , Luan Jiaochen , Zhang Qijie , Zhang Xu , Ren Xiaohan , Zhang Tongtong , Meng Xianghu , Song Ninghong
Published: July 2022

Background

The key regulatory roles of long non-coding RNAs (lncRNAs) in age-related erectile dysfunction (A-ED) are unknown.

Aim

This study aimed to identify putative lncRNAs that regulate age-related erectile dysfunction via transcriptome analyses, and to predict their specific regulatory routes via bioinformatics methods.

Methods

22 geriatric male SD rats were divided into age-related erectile dysfunction (A-ED) and negative control (NC) groups after evaluations of intracavernous pressure (ICP). By comparative analysis of transcriptomes of cavernosal tissues from both groups, we identified differentially expressed lncRNAs, miRNAs, and mRNAs. Seven differentially expressed lncRNAs were selected and further verified by quantitative real-time polymerase chain reactions (RT-qPCR). The construction of the lncRNA-miRNA-mRNA network, the Gene Ontology (GO) term enrichment, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed in Cytoscape.

Results

From comparative transcriptome analyses of A-ED and NC groups, 69, 29, and 364 differentially expressed lncRNAs, miRNAs, and mRNAs were identified respectively. Differentially expressed lncRNAs were culled to seven, which were all verified by qPCR. Three of these lncRNAs (ENSRNOT00000090050, ENSRNOT00000076482, and ENSRNOT00000029245) were used to build regulatory networks, of which only ENSRNOT00000029245 was successful. Moreover, GO and KEGG analyses demonstrated that these lncRNAs possibly regulated muscle myosin complex, muscle cell cellular homeostasis, and ultimately erectile function in rats through PI3K-Akt, fluid shear stress, and atherosclerosis pathways.

Conclusion

Our study identified differentially expressed lncRNAs, miRNAs, and mRNAs through comparisons of transcriptomes of geriatric rats. An identified lncRNA verified by qPCR, was used to construct a lncRNA-miRNA-mRNA regulatory network. LncRNA ENSRNOT00000029245 possibly regulated downstream mRNAs through this regulatory network, leading to apoptosis in the cavernous tissue, fibrosis, and endothelial dysfunction, which ultimately caused ED. These findings provide seminal insights into the molecular biology of aging-related ED, which could spur the development of effective therapeutics.