bluuebunny/crossref_metadata_2025 huggingface dataset:
Chain Substituted Cannabilactones with Selectivity for the CB2 Cannabinoid Receptor
Authors: Alapafuja Shakiru O. , Nikas Spyros P. , Ho Thanh C. , Tong Fei , Benchama Othman , Makriyannis Alexandros
Published: October 2019

In earlier work, we reported a novel class of CB2 selective ligands namely cannabilactones. These compounds carry a dimethylheptyl substituent at C3, which is typical for synthetic cannabinoids. In the current study with the focus on the pharmacophoric side chain at C3 we explored the effect of replacing the C1′-gem-dimethyl group with the bulkier cyclopentyl ring, and, we also probed the chain’s length and terminal carbon substitution with bromo or cyano groups. One of the analogs synthesized namely 6-[1-(1,9-dihydroxy-6-oxo-6H-benzo[c]chromen-3-yl) cyclopentyl] hexanenitrile (AM4346) has very high affinity (Ki = 4.9 nM) for the mouse CB2 receptor (mCB2) and 131-fold selectivity for that target over the rat CB1 (rCB1). The species difference in the affinities of AM4346 between the mouse (m) and the human (h) CB2 receptors is reduced when compared to our first-generation cannabilactones. In the cyclase assay, our lead compound was found to be a highly potent and efficacious hCB2 receptor agonist (EC50 = 3.7 ± 1.5 nM, E(max) = 89%). We have also extended our structure-activity relationship (SAR) studies to include biphenyl synthetic intermediates that mimic the structure of the phytocannabinoid cannabinodiol.
Quantitative Analysis of Organic Liquid Three-Component Systems: Near-Infrared Transmission versus Raman Spectroscopy, Partial Least Squares versus Classical Least Squares Regression Evaluation and Volume versus Weight Percent Concentration Units
Authors: Yan Hui , Ma Yue , Xiong Zhixin , Siesler Heinz W. , Qi Liang , Zhang Guozheng
Published: October 2019

The band shapes and band positions of near-infrared (NIR) and Raman spectra change depending on the concentrations of specific chemical functionalities in a multicomponent system. To elucidate these effects in more detail and clarify their impact on the analytical measurement techniques and evaluation procedures, NIR transmission spectra and Raman spectra of two organic liquid three-component systems with variable compositions were analyzed by two different multivariate calibration procedures, partial least squares (PLS) and classical least-squares (CLS) regression. Furthermore, the effect of applying different concentration units (volume percent (%V) and weight percent (%W) on the performance of the two calibration procedures have been tested. While the mixtures of benzene/cyclohexane/ethylbenzene (system 1) can be regarded as a blended system with comparatively low molecular interactions, hydrogen bonding plays a dominant role in the blends of ethyl acetate/1-heptanol/1,4-dioxane (system 2). Whereas system 1 yielded equally good calibrations by PLS and CLS regression, for system 2 acceptable results were only obtained by PLS regression. Additionally, for both sample systems, Raman spectra generally led to lower calibration performance than NIR spectra. Finally, volume and weight percent concentration units yielded comparable results for both chemometric evaluation procedures.
Isolation, Maintenance and Differentiation of Primary Tracheal Basal Cells from Adult Rhesus Macaque
Authors: Engler Anna E. , Mostoslavsky Gustavo , Miller Lisa , Rock Jason R.
Published: October 2019

In this report, we describe methodologies for the isolation and culture of primary rhesus macaque tracheal basal cells, their cryopreservation, long term storage and differentiation. These are comparable to state-of-the-art protocols that have been developed for mouse and human airway basal cells. This method is based on the use of proprietary media, providing an easily reproducible and applicable protocol for usage in biosafety level 2 (BSL2) settings. Tracheas from rhesus macaques were isolated after animal euthanasia and subjected to enzymatic digestion overnight. Cells of the epithelial layer were scraped off of the trachea for cell culture. Twenty-four hours after plating basal cells had attached and nonadherent cells were removed. First passages of basal cells can be frozen for early passage storage in liquid nitrogen or propagated and differentiated on an air–liquid interface and in a tracheosphere assay up to passage seven. This protocol provides a platform for the analysis of basal cells from a close evolutionary relative to humans.
Comparison of Free-Space and Waveguide-Based SERS Platforms
Authors: Turk Nina , Raza Ali , Wuytens Pieter , Demol Hans , Van Daele Michiel , Detavernier Christophe , Skirtach Andre , Gevaert Kris , Baets Roel
Published: October 2019

Surface-Enhanced Raman Spectroscopy (SERS) allows for the highly specific detection of molecules by enhancing the inherently weak Raman signals near the surface of plasmonic nanostructures. A variety of plasmonic nanostructures have been developed for SERS signal excitation and collection in a conventional free-space microscope, among which the gold nanodomes offer one of the highest SERS enhancements. Nanophotonic waveguides have recently emerged as an alternative to the conventional Raman microscope as they can be used to efficiently excite and collect Raman signals. Integration of plasmonic structures on nanophotonic waveguides enables reproducible waveguide-based excitation and collection of SERS spectra, such as in nanoplasmonic slot waveguides. In this paper, we compare the SERS performance of gold nanodomes, in which the signal is excited and collected in free space, and waveguide-based nanoplasmonic slot waveguide. We evaluate the SERS signal enhancement and the SERS background of the different SERS platforms using a monolayer of nitrothiophenol. We show that the nanoplasmonic slot waveguide approaches the gold nanodomes in terms of the signal-to-background ratio. We additionally demonstrate the first-time detection of a peptide monolayer on a waveguide-based SERS platform, paving the way towards the SERS monitoring of biologically relevant molecules on an integrated lab-on-a-chip platform.
A Comprehensive Peptidomic Approach to Characterize the Protein Profile of Selected Durum Wheat Genotypes: Implication for Coeliac Disease and Wheat Allergy
Authors: Pilolli Rosa , Gadaleta Agata , Di Stasio Luigia , Lamonaca Antonella , De Angelis Elisabetta , Nigro Domenica , De Angelis Maria , Mamone Gianfranco , Monaci Linda
Published: October 2019

The wheat varietal selection undertaken by breeders in recent decades has been tailored mainly to improve technological and productivity-related traits; however, the latter has resulted in a considerable impoverishment of the genetic diversity of wheat-based products available on the market. This pitfall has encouraged researchers to revalue the natural diversity of cultivated and non-cultivated wheat genotypes in light of their different toxic/immunogenic potential for celiac disease and wheat-allergic patients. In the present investigation, an advanced proteomic approach was designed for the global characterization of the protein profile of selected tetraploid wheat genotypes (Triticum turgidum). The approach combined proteins/peptides sequence information retrieved by specific enzymatic digestions (single and dual proteolytic enzymes) with protein digestibility information disclosed by means of in-vitro simulated human gastroduodenal digestion experiments. In both cases, the peptide pools were characterized by discovery analysis with liquid chromatography high-resolution tandem mass spectrometry, and specific amino acid sequences were identified via commercial software. The peptide list was screened for in silico toxicity/immunogenicity risk assessment, with the aid of various open-source bioinformatics tools for epitopes matching. Given the global information provided by the designed proteomic approach, the in silico risk assessment not only tackled toxicity implication for celiac disease patients, but also scouted for immunogenic sequences relevant for wheat allergic patients, achieving a comprehensive characterization of the protein profile of the selected genotypes. These latter were assessed to encrypt a variable number of toxic/immunogenic epitopes for celiac disease and wheat allergy, and as such they could represent convenient bases for breeding practices and for the development of new detoxification strategies.
Search and Selection of Probiotics That Improve Mucositis Symptoms in Oncologic Patients. A Systematic Review
Authors: Picó-Monllor José Antonio , Mingot-Ascencao José Manuel
Published: October 2019

Mucositis is a common and severe adverse effect of radiotherapy and/or chemotherapy treatments applied to oncologic patients. The development of effective therapies and adjuvant treatments to increase their efficacy and reduce adverse effect is a priority in cancer therapy. Probiotics are non-pathogenic live microorganisms that when ingested in adequate amounts can colonize the intestinal tract promoting the restoration of a healthy gut microbiota and contributing to all its functions including the maintenance of the integrity of the mucosa and the modulation of the immune system. In order to check the possible efficacy and safety of these microorganisms to prevent or ameliorate mucositis′ symptoms, we have systematically searched the bibliographic databases MEDLINE (via Pubmed), EMBASE, The Cochrane library, Scopus, Web of science, and Latin American and Caribbean Literature in Health of Sciences (LILACS) using the descriptors “Mucositis”, “Probiotics”, “Neoplasms”, “Humans”, and “Clinical Trials”. After applying our inclusion and exclusion criteria, 15 studies were accepted for review and critical analysis. Our analysis suggests that a combination of Bifidobacterium longum, Lactobacillus acidophilus, Bifidobacterium breve, Bifidobacterium infantis, and Saccharomyces boulardii could be a good combination of probiotics to reduce incident rates of mucositis or ameliorate its symptoms in chemo or radiotherapy treated patients.
Nutritional Status in Spanish Children and Adolescents with Celiac Disease on a Gluten Free Diet Compared to Non-Celiac Disease Controls
Authors: Fernández Catalina Ballestero , Varela-Moreiras Gregorio , Úbeda Natalia , Alonso-Aperte Elena
Published: October 2019

Patients who follow a gluten-free diet (GFD) may be prone to nutritional deficiencies, due to food restriction and consumption of gluten-free products. The aim was to assess nutritional status in celiac children and adolescents on a long-term GFD. A cross-sectional age and gender-matched study in 70 celiac and 67 non-celiac volunteers was conducted, using dietary, anthropometric, biochemical parameters, and assessing bone mineral density and physical activity. Adequacy of vitamin D intake to recommendations was very low, in both groups, and intakes for calcium and magnesium were significantly lower in celiac volunteers. Celiac children and adolescents may have a higher risk of iron and folate deficiencies. Both groups followed a high-lipid, high-protein, low fiber diet. Median vitamin D plasma levels fell below reference values, in celiac and non-celiac participants, and were significantly lower in celiac girls. Other biochemical parameters were within normal ranges. Anthropometry and bone mineral density were similar within groups. With the exception of some slightly lower intakes, children and adolescents following a GFD appear to follow the same trends as healthy individuals on a normal diet. No effect of food restriction or gluten-free product consumption was observed.
Cholesterol versus Inflammation as Cause of Chronic Diseases
Authors: Panchal Sunil K. , Brown Lindsay
Published: October 2019

Tsoupras and colleagues have postulated, in a recent review in Nutrients, that the key to reducing the incidence of cardiovascular disease is to control the activities of inflammatory mediators such as platelet-activating factor (PAF) by diet, exercise, and healthy lifestyle choices [...]
Inhalable Dry Powder of Bedaquiline for Pulmonary Tuberculosis: In Vitro Physicochemical Characterization, Antimicrobial Activity and Safety Studies
Authors: Momin Mohammad A. M. , Rangnekar Bhamini , Sinha Shubhra , Cheung Chen-Yi , Cook Gregory M. , Das Shyamal C.
Published: October 2019

Bedaquiline is a newly developed anti-tuberculosis drug, conditionally approved by the United States Food and Drug Administration (USFDA) for treating drug-resistant tuberculosis in adults. Oral delivery of bedaquiline causes severe side effects such as increased hepatic aminotransferase levels and cardiac arrhythmias (prolongation of QT-interval). This study aimed to develop inhalable dry powder particles of bedaquiline with high aerosolization efficiency to reduce the side-effects of oral bedaquiline. Bedaquiline (with or without l-leucine) powders were prepared using a Buchi Mini Spray-dryer. The powders were characterized for physicochemical properties and for their in vitro aerosolization efficiency using a next-generation impactor (NGI). The formulation with maximum aerosolization efficiency was investigated for physicochemical and aerosolization stability after one-month storage at 20 ± 2 °C/30 ± 2% relative humidity (RH) and 25 ± 2 °C/75% RH in an open Petri dish. The cytotoxicity of the powders on A549 and Calu-3 cell-lines was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The powders were also evaluated for antimicrobial activity against Mycobacterium tuberculosis. The aerodynamic diameter of the l-leucine-containing powder was 2.4 µm, and the powder was amorphous in nature. The aerosolization efficiency (fine-particle fraction) of l-leucine-containing powder (fine-particle fraction (FPF): 74.4%) was higher than the bedaquiline-only powder (FPF: 31.3%). l-leucine containing powder particles were plate-shaped with rough surfaces, but the bedaquiline-only powder was spherical and smooth. The optimized powder was stable at both storage conditions during one-month storage and non-toxic (up to 50 µg/mL) to the respiratory cell-lines. Bedaquiline powders were effective against Mycobacterium tuberculosis and had a minimal inhibitory concentration (MIC) value of 0.1 µg/mL. Improved aerosolization may help to combat pulmonary tuberculosis by potentially reducing the side-effects of oral bedaquiline. Further research is required to understand the safety of the optimized inhalable powder in animal models.
Assessment of the Inter-Batch Variability of Microstructure Parameters in Topical Semisolids and Impact on the Demonstration of Equivalence
Authors: Mangas-Sanjuán Víctor , Pleguezuelos-Villa María , Merino-Sanjuán Matilde , Hernández Mª Jesús , Nácher Amparo , García-Arieta Alfredo , Peris Daniel , Hidalgo Irene , Soler Lluís , Sallan Marta , Merino Virginia
Published: October 2019

Demonstration of similar microstructure is essential for demonstrating the equivalence of generic topical products since the microstructure of semisolids may affect the drug release. The objective of this study was to compare the microstructure-defining physical parameters of different batches of a reference ointment containing calcipotriol and betamethasone (Daivobet 50 µg/0.5 mg/g) in order to define the acceptance range that allows concluding equivalence between these batches. Being batches of the same reference product, they are expected to be clinically equivalent and possess similar microstructure. The 90% confidence intervals for the test/reference ratio of these physical parameters were calculated with parametric and non-parametric approaches. Both methods conclude that equivalent microstructure between batches cannot be demonstrated with a reasonable sample size when the acceptance range was set at ±10%, since several physical parameters exhibit inter-batch variability >10%. An acceptance range of ±10% is therefore too strict to conclude equivalence in the microstructure of semisolid dosage forms, given the inter-batch variability observed between batches of the reference product. A wider fixed acceptance range or an acceptance range widened based on the inter-batch variability of the reference product would be advisable.
Influence of Short Cationic Lipopeptides with Fatty Acids of Different Chain Lengths on Bacterial Biofilms Formed on Polystyrene and Hydrogel Surfaces
Authors: Paduszynska Malgorzata Anna , Maciejewska Magdalena , Neubauer Damian , Golacki Krzysztof , Szymukowicz Magdalena , Bauer Marta , Kamysz Wojciech
Published: October 2019

Nowadays, biomaterials are applied in many different branches of medicine. They significantly improve the patients’ comfort and quality of life, but also constitute a significant risk factor for biofilm-associated infections. Currently, intensive research on the development of novel materials resistant to microbial colonization as well as new compounds that are active against biofilms is being carried out. Within this research, antimicrobial peptides (AMPs) and their analogues are being intensively investigated due to their promising antimicrobial activities. The main goal of this study was to synthesize and evaluate the antimicrobial efficacy of short cationic lipopeptides that were designed to imitate the features of AMPs responsible for antimicrobial activities: positive net charge and amphipacity. The positive charge of the molecules results from the presence of basic amino acid residues: arginine and lysine. Amphipacity is provided by the introduction of decanoic, dodecanoic, tetradecanoic, and hexadecanoic acid chains to the molecules. Lipopeptides (C16-KR-NH2, C16-KKK-NH2, C16-KKC-NH2, C16-KGK-NH2, C14-KR-NH2, C14-KKC-NH2, C12-KR-NH2, C12-KKC-NH2, and (C10)2-KKKK-NH2) were synthesized using a novel solid-phase temperature-assisted methodology. The minimum inhibitory concentrations (MICs), minimum biofilm eradication concentrations (MBECs), and minimum biofilm formation inhibitory concentrations (MBFICs) were determined for the following bacterial strains: Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 14990, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027, and Proteus mirabilis PCM 543. The biofilms were cultured on two types of surfaces: polystyrene plates (PS) and contact lenses (CL). The lipopeptides exhibited the ability to inhibit the growth of bacteria in a liquid medium as well as on the PS and CL. The compounds also eliminated the bacterial biofilm from the surface of both materials. In general, the activity against gram-positive bacteria was stronger in comparison to that against gram-negative strains. There were certain discrepancies between the activity of compounds against the biofilm cultured on PS and CL. This was especially noticeable for staphylococci—the lipopeptides presented much higher activity against biofilm formed on the PS surface. It is worth noting that the obtained MBEC values for lipopeptides were usually only a few times higher than the MICs. The results of the performed experiments suggest that further studies on lipopeptides and their potential application in the treatment and prophylaxis of biofilm-associated infections should be conducted.
Polydopamine-Based “Four-in-One” Versatile Nanoplatforms for Targeted Dual Chemo and Photothermal Synergistic Cancer Therapy
Authors: Liu Gan , Gao Nansha , Zhou Yun , Nie Junpeng , Cheng Wei , Luo Miaomiao , Mei Lin , Zeng Xiaowei , Deng Wenbin
Published: October 2019

The development of versatile nanoscale drug delivery systems that integrate with multiple therapeutic agents or methods and improve the efficacy of cancer therapy is urgently required. To satisfy this demand, polydopamine (PDA)-modified polymeric nanoplatforms were constructed for the dual loading of chemotherapeutic drugs. The hydrophobic anticancer drug docetaxel (DTX) was loaded into the polymeric nanoparticles (NPs) which were fabricated from the star-shaped copolymer CA-PLGA. Then DTX-loaded NPs were coated with PDA, followed by conjugation of polyelethyl glycol (PEG)-modified targeting ligand aptamer AS1411(Apt) and adsorption of the hydrophilic anticancer drug doxorubicin (DOX). This “four-in-one” nanoplatform, referred to as DTX/NPs@PDA/DOX-PEG-Apt, demonstrated high near-infrared photothermal conversion efficiency and exhibited pH and thermo-responsive drug release behavior. Furthermore, it was able to specifically target MCF-7 human breast carcinoma cells and provide synergistic chemo-photothermal therapy to further improve the anticancer effect both in vitro and in vivo, providing a novel promising strategy for cancer therapy.
De Novo Assembly Discovered Novel Structures in Genome of Plastids and Revealed Divergent Inverted Repeats in Mammillaria (Cactaceae, Caryophyllales)
Authors: Solórzano Sofía , Chincoya Delil A. , Sanchez-Flores Alejandro , Estrada Karel , Díaz-Velásquez Clara E. , González-Rodríguez Antonio , Vaca-Paniagua Felipe , Dávila Patricia , Arias Salvador
Published: October 2019

The complete sequence of chloroplast genome (cpDNA) has been documented for single large columnar species of Cactaceae, lacking inverted repeats (IRs). We sequenced cpDNA for seven species of the short-globose cacti of Mammillaria and de novo assembly revealed three novel structures in land plants. These structures have a large single copy (LSC) that is 2.5 to 10 times larger than the small single copy (SSC), and two IRs that contain strong differences in length and gene composition. Structure 1 is distinguished by short IRs of <1 kb composed by rpl23-trnI-CAU-ycf2; with a total length of 110,189 bp and 113 genes. In structure 2, each IR is approximately 7.2 kb and is composed of 11 genes and one Intergenic Spacer-(psbK-trnQ)-trnQ-UUG-rps16-trnK-UUU-matK-trnK-UUU-psbA-trnH-GUG-rpl2-rpl23-trnI-CAU-ycf2; with a total size of 116,175 bp and 120 genes. Structure 3 has divergent IRs of approximately 14.1 kb, where IRA is composed of 20 genes: psbA-trnH-GUG-rpl23-trnI-CAU-ycf2-ndhB-rps7-rps12-trnV-GAC-rrn16-ycf68-trnI-GAU-trnA-AGC-rrn23-rrn4.5-rrn5-trnR-ACG-trnN-GUU-ndhF-rpl32; and IRB is identical to the IRA, but lacks rpl23. This structure has 131 genes and, by pseudogenization, it is shown to have the shortest cpDNA, of just 107,343 bp. Our findings show that Mammillaria bears an unusual structural diversity of cpDNA, which supports the elucidation of the evolutionary processes involved in cacti lineages.
Critical Factors for the Recycling of Different End-of-Life Materials: Wood Wastes, Automotive Shredded Residues, and Dismantled Wind Turbine Blades
Authors: Castaldo Rachele , De Falco Francesca , Avolio Roberto , Bossanne Emilie , Cicaroni Fernandes Felipe , Cocca Mariacristina , Di Pace Emilia , Errico Maria Emanuela , Gentile Gennaro , Jasiński Dominik , Spinelli Daniele , Albein Urios Sonia , Vilkki Markku , Avella Maurizio
Published: October 2019

Different classes of wastes, namely wooden wastes, plastic fractions from automotive shredded residues, and glass fiber reinforced composite wastes obtained from dismantled wind turbines blades were analyzed in view of their possible recycling. Wooden wastes included municipal bulky wastes, construction and demolition wastes, and furniture wastes. The applied characterization protocol, based on Fourier transform infrared (FTIR) spectroscopy in attenuated total reflection (ATR) mode, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM/EDX), and thermogravimetric analysis (TG) coupled with FTIR spectrometry for the investigation of the evolved gases, revealed that the selected classes of wastes are very complex and heterogeneous materials, containing different impurities that can represent serious obstacles toward their reuse/recycling. Critical parameters were analyzed and discussed, and recommendations were reported for a safe and sustainable recycling of these classes of materials.
Antifungal Activity of Euclea divinorum Root and Study of its Ethnobotany and Phytopharmacology
Authors: Al-Fatimi Mohamed
Published: October 2019

The ethnobotanical survey of Euclea divinorum Hiern (Ebenaceae) was conducted on Soqotra Island, Yemen. The root bark is used to treat mouth, dental, dermal and blood diseases in the traditional medicine of the island. The study is the first report about the effect of the plant root barks against six human pathogenic fungi. The non-polar dichloromethane extract of Euclea divinorum root bark showed stronger antifungal activities compared to polar direct and sequential methanolic extracts. These extracts showed significant broad antifungal activity against Absidia corymbifera, Aspergillus fumigatus, Candida krusei, Microsporum gypseum, Mucor sp. and Trichophyton mentagrophytes compared to the standard antibiotic drug nystatin. Thin-layer chromatography (TLC) revealed the presence of the naphthoquinones in the extracts. The results showed an extraction process to separate most antifungal naphthoquinones from the root bark by using non-polar solvent dichloromethane, while flavonoids remained in the polar methanolic extracts; therefore, the polar sequential and direct methanolic extracts recorded higher antioxidant activity than the non-polar extracts with less toxicity. The biological and chemical results identified the presence of antifungal and antioxidant constituents in the root bark and confirm its traditional use in Soqotra Island as crude powder to treat dental and dermal diseases and to clean teeth. Moreover, our results are compared with literature review on ethnobotany and phytopharmacology of E. divinorum to present a medicinal monograph about the plant.
Landscape Fragmentation, Ecosystem Services, and Local Knowledge in the Baroro River Watershed, Northern Philippines
Authors: Ramirez Mark Anthony M. , Pulhin Juan M. , Garcia Josephine E. , Tapia Maricel A. , Pulhin Florencia B. , Cruz Rex Victor O. , De Luna Catherine C. , Inoue Makoto
Published: October 2019

Landscape fragmentation, the breaking up of land use type into smaller parcels, is damaging watersheds worldwide. Without addressing its causes, landscape fragmentation can permanently destroy habitats and compromise ecosystem services (ES) that a watershed provides. This paper aims to establish associations between watershed landscape fragmentation and ES by integrating science (satellite imageries and fragmentation analyses) and local geographic knowledge (key informant interviews and focus group discussions) at different time periods. Using the case of the Baroro River Watershed in Northern Philippines, this paper posits that local knowledge, when integrated with scientific knowledge, becomes a significant medium through which watershed landscape fragmentation and declining quality of ES can be better understood and addressed. Results also indicate that people’s experiences and knowledge on ES coincide with watershed landscape fragmentation as evidenced by satellite images and fragmentation analyses done at different time periods. This implies that people’s knowledge is well grounded on facts and complements scientific knowledge necessary in crafting more effective landscape policies that can tackle watershed fragmentation. Study results are also crucial in providing information to serve as inputs in the development of a more robust watershed management plan; particularly in implementing sustainable land uses without sacrificing the watershed’s overall integrity.
Detection of Earthquake-Induced Landslides during the 2018 Kumamoto Earthquake Using Multitemporal Airborne Lidar Data
Authors: Liu Wen , Yamazaki Fumio , Maruyama Yoshihisa
Published: October 2019

A series of earthquakes hit Kumamoto Prefecture, Japan, continuously over a period of two days in April 2016. The earthquakes caused many landslides and numerous surface ruptures. In this study, two sets of the pre- and post-event airborne Lidar data were applied to detect landslides along the Futagawa fault. First, the horizontal displacements caused by the crustal displacements were removed by a subpixel registration. Then, the vertical displacements were calculated by averaging the vertical differences in 100-m grids. The erosions and depositions in the corrected vertical differences were extracted using the thresholding method. Slope information was applied to remove the vertical differences caused by collapsed buildings. Then, the linked depositions were identified from the erosions according to the aspect information. Finally, the erosion and its linked deposition were identified as a landslide. The results were verified using truth data from field surveys and image interpretation. Both the pair of digital surface models acquired over a short period and the pair of digital terrain models acquired over a 10-year period showed good potential for detecting 70% of landslides.
Rain Monitoring with Polarimetric GNSS Signals: Ground-Based Experimental Research
Authors: An Hao , Yan Wei , Bian Shuangshuang , Ma Shuo
Published: October 2019

In recent years, there has been a preliminary research on monitoring rainfall information based on polarimetric Global Navigation Satellite System (GNSS) signals, which is a quite novel concept. After previous theoretical research on monitoring rain based on polarimetric phase shift of GNSS signals, the paper aims to detect rain using polarimetric GNSS signals from a ground-based experiment. Firstly, a conical horn antenna specially designed for receiving dual-polarized (H, horizontal, and V, vertical) GNSS signals was developed, and an experimental system for polarimetric GNSS rain detection was built. Then, taking Global Positioning System (GPS) satellites as signal source, a ground-based experiment was carried out at a mountain in Nanjing, where heavy rain tends to occur frequently in rainy season. Additionally, a data processing algorithm mainly following Padullés et al. (2016) to solve the problems of quality control, unlocking, hardware effect, phase ambiguity, multipath effect was applied independently to this ground-based data from the polarimetric GNSS rain detection system. Also, the multi-source data from nearby weather radar and weather stations was used for verification. Results from 14 GPS satellites show that the obtained phase shift is zero in all no-rain days while it is not zero during rainy days, which is in accordance with the actual situation. Compared with weather radar and rain gauges’ data, the results verify that the phase shift is caused by rain. Besides, when individual cases are examined, many show that their tendencies of accumulated phase shift are quite similar to that of a weather station’s rainfall data, even some correlation coefficients are up to 0.99. These demonstrate the reliability of our experimental system and the feasibility of the data processing algorithm. This study will provide technical support for future spaceborne experiment, which has promising applications in global rain monitoring.
CYGNSS Surface Heat Flux Product Development
Authors: Crespo Juan , Posselt Derek , Asharaf Shakeel
Published: October 2019

Ocean surface heat fluxes play a significant role in the genesis and evolution of various marine-based atmospheric phenomena, from the synoptic scale down to the microscale. While in-situ measurements from buoys and flux towers will continue to be the standard in regard to surface heat flux estimates, they commonly have significant gaps in temporal and spatial coverage. Previous and current satellite missions have filled these gaps; though they may not observe the fluxes directly, they can measure the variables needed (wind speed, temperature and humidity) to estimate latent and sensible heat fluxes. However, current remote sensing instruments have their own limitations, such as infrequent coverage, signals attenuated by precipitation or both. The Cyclone Global Navigation Satellite System (CYGNSS) mission overcomes these limitations over the tropical and subtropical oceans by providing improved coverage in nearly all weather conditions. While CYGNSS (Level 2) primarily estimates surface winds, when coupled with observations or estimates of temperature and humidity from reanalysis data, it can provide estimates of latent and sensible heat fluxes along its orbit. This paper describes the development of the Surface Heat Flux Product for the CYGNSS mission, its current results and expected improvements and changes in future releases.
Target-Specific Action Classification for Automated Assessment of Human Motor Behavior from Video
Authors: Rezaei Behnaz , Christakis Yiorgos , Ho Bryan , Thomas Kevin , Erb Kelley , Ostadabbas Sarah , Patel Shyamal
Published: October 2019

Objective monitoring and assessment of human motor behavior can improve the diagnosis and management of several medical conditions. Over the past decade, significant advances have been made in the use of wearable technology for continuously monitoring human motor behavior in free-living conditions. However, wearable technology remains ill-suited for applications which require monitoring and interpretation of complex motor behaviors (e.g., involving interactions with the environment). Recent advances in computer vision and deep learning have opened up new possibilities for extracting information from video recordings. In this paper, we present a hierarchical vision-based behavior phenotyping method for classification of basic human actions in video recordings performed using a single RGB camera. Our method addresses challenges associated with tracking multiple human actors and classification of actions in videos recorded in changing environments with different fields of view. We implement a cascaded pose tracker that uses temporal relationships between detections for short-term tracking and appearance based tracklet fusion for long-term tracking. Furthermore, for action classification, we use pose evolution maps derived from the cascaded pose tracker as low-dimensional and interpretable representations of the movement sequences for training a convolutional neural network. The cascaded pose tracker achieves an average accuracy of 88% in tracking the target human actor in our video recordings, and overall system achieves average test accuracy of 84% for target-specific action classification in untrimmed video recordings.
Spatial Distortion Assessments of a Low-Cost Laboratory and Field Hyperspectral Imaging System
Authors: Krtalić Andrija , Miljković Vanja , Gajski Dubravko , Racetin Ivan
Published: October 2019

This article describes the adaptation of an existing aerial hyperspectral imaging system in a low-cost setup for collecting hyperspectral data in laboratory and field environment and spatial distortion assessments. The imaging spectrometer system consists of an ImSpector V9 hyperspectral pushbroom scanner, PixelFly high performance digital CCD camera, and a subsystem for navigation, position determination and orientation of the system in space, a sensor bracket and control system. The main objective of the paper is to present the system, with all its limitations, and a spatial calibration method. The results of spatial calibration and calculation of modulation transfer function (MTF) are reported along with examples of images collected and potential uses in agronomy. The distortion value rises drastically at the edges of the image in the near-infrared segment, while the results of MTF calculation showed that the image sharpness was equal for the bands from the visible part of the spectrum, and approached Nyquist’s theory of digitalization. In the near-infrared part of the spectrum, the MTF values showed a less sharp decrease in comparison with the visible part. Preliminary image acquisition indicates that this hyperspectral system has potential in agronomic applications.
Integrated Laser Sensor (ILS) for Remote Surface Analysis: Application for Detecting Explosives in Fingerprints
Authors: Lazic Violeta , Palucci Antonio , De Dominicis Luigi , Nuvoli Marcello , Pistilli Marco , Menicucci Ivano , Colao Francesco , Almaviva Salvatore
Published: October 2019

Here, we describe an innovative Integrated Laser Sensor (ILS) that combines four spectroscopic techniques and two vision systems into a unique, transportable device. The instrument performs Raman and Laser-Induced Fluorescence (LIF) spectroscopy excited at 355 nm and Laser-Induced Breakdown Spectroscopy (LIBS) excited at 1064 nm, and it also detects Laser Scattering (LS) from the target under illumination at 650 nm. The combination of these techniques supplies information about: material change from one scanning point to another, the presence of surface contaminants, the molecular and elemental composition of top target layers. Switching between the spectroscopic techniques and the laser wavelengths is fully automatic. The instrument is equipped with an autofocus and it performs scanning with a chosen grid density over an interactively-selected target area. Alternative to the spectroscopic measurements, it is possible to switch the instrument to a high magnification target viewing. The working distances tested until now are between 8.5 and 30 m. The instrument is self-powered and remotely controlled via wireless communication. The ILS has been fully developed at ENEA for security applications and it was successfully tested in two outdoor campaigns where an automatic recognition of areas containing explosives in traces had been implemented. The strategies for the identification of nitro-compounds placed on various substrates as fingerprints and the results obtained at a working distance of 10 m are discussed in the following.
Isometric Posterior Chain Peak Force Recovery Response Following Match-Play in Elite Youth Soccer Players: Associations with Relative Posterior Chain Strength
Authors: Constantine Emma , Taberner Matt , Richter Chris , Willett Matthew , Cohen Daniel D.
Published: October 2019

The purpose of this study was to determine changes in two tests of lower limb isometric posterior chain force (IPC-F) following 90 min of match-play in elite youth soccer players and the interaction between relative strength and recovery profile. 14 players (age: 16 ± 2 years) performed 3 × 3 second IPC-F tests unilaterally at 30° and 90° of knee and hip flexion pre- and post-match, +24 h, +48 h, and +72 h post-match. Peak force was recorded for both limbs, combined and expressed relative to bodyweight (N/kg). A two-way repeated measures analysis of variance was performed to determine differences in force output between joint angles, time intervals and subjects. As there was no interaction between angle and time (p = 0.260), we report the change between timepoints as mean ∆ in 90° + 30° IPC-F. Relative to pre-match IPC-F, there were significant decreases post (∆ = −18%; p > 0.001) and at +24 h (∆ = −8%; p = 0.040), no significant difference at +48 h (∆ = 0%; p = 0.992) and a significant increase at +72 h (∆ = +12%; p = 0.005). There was a large inter-individual variability in recovery profile at both angles and substantial differences between post-match deficits at 90° (−10.8%) compared to 30° (−20.7%). Higher pre-match IPC-F was correlated with the magnitude of IPC-F deficits at both angles and all time points (r = 0.56 to 0.70, p = < 0.01) except for post-match 90°. Regular IPC-F monitoring to determine the magnitude of match-induced fatigue and track recovery may help inform decision-making regarding modifications to individual players training load, particularly as there is a large inter-individual variability in response to competition. Further research is warranted to better understand and address the finding that stronger players showed larger force deficits and slower recovery following match-play.
City Brand Personality Projected by Municipalities from Central and Eastern Europe Countries—A Comparison of Facebook Usage
Authors: Glińska Ewa , Rudolf Wawrzyniec
Published: October 2019

City branding is becoming more and more popular, not only as a field of research, but also as a practice of local governments. Municipalities attempt to introduce this concept into their sustainable transformation process, positioning a city among its stakeholders as, for instance, an eco-oriented, innovative, or smart city. An idea that fosters building a strong and consistent city brand is brand personality. The aim of the paper is to diagnose a city’s brand personality dimensions/traits communicated via posts published on official Facebook accounts of cities and to identify differences occurring within this scope between cities of two selected Central and Eastern European countries—Poland and Ukraine. To achieve this aim, the research involved a content analysis of 1700 posts published by 34 municipalities representing regional capital cities of Poland and Ukraine. On the basis of these study results, the authors have supplemented the Aaker (1997) five-dimensional scale of brand personality with the sixth dimension: Prosocial Attitude, which includes cooperative and bureaucratic traits, to better comply with the approach of cities to problems of their inhabitants and their surrounding environment, which is consistent with the idea of sustainability. Furthermore, the fifth trait has been added to characteristics which make up Aaker’s Excitement dimension. Dubbed as patriotic, it refers to the city’s activities related to the local celebration of historical events, indicating national pride and identification with its place of residence. Bureaucratic and patriotic traits were quite frequently observed in posts published on the official Facebook accounts of Ukrainian cities. Moreover, the research proves that in case of Polish cities the dominating brand personality dimensions are Sincerity and Excitement. Ukrainian cities tend to exercise communication based on the Sincerity and Competence dimension.
Multi-Objective Sizing of Hybrid Energy Storage System for Large-Scale Photovoltaic Power Generation System
Authors: Ma Chao , Dong Sen , Lian Jijian , Pang Xiulan
Published: October 2019

Hybrid energy storage systems (HESS) are an effective way to improve the output stability for a large-scale photovoltaic (PV) power generation systems. This paper presents a sizing method for HESS-equipped large-scale centralized PV power stations. The method consists of two parts: determining the power capacity by a statistical method considering the effects of multiple weather conditions and calculating the optimal energy capacity by employing a mathematical model. The method fully considers the characteristics of PV output and multiple kinds of energy storage combinations. Additionally, a pre-storage strategy that can further improve stability of output is proposed. All of the above methods were verified through a case study application to an 850 MW centralized PV power station in the upstream of the Yellow river. The optimal hybrid energy storage combination and its optimization results were obtained by this method. The results show that the optimal capacity configuration can significantly improve the stability of PV output and the pre-storage strategy can further improve the target output satisfaction rate by 8.28%.
In-Depth Characterisation of Common Bean Diversity Discloses Its Breeding Potential for Sustainable Agriculture
Authors: Caproni Leonardo , Raggi Lorenzo , Ceccarelli Salvatore , Negri Valeria , Carboni Andrea
Published: October 2019

Legumes’ cultivation contributes services to agro-ecosystems and society, in line with the principles of sustainability. Among pulses, the common bean is one of the most important sources of plant proteins and other important nutrients for humans. Extensive phenotypic and genetic characterisations of unexplored bean germplasm are still needed to unlock its breeding potential. To the purpose, a panel of 192 diverse genotypes, mainly developed starting from European landrace accessions, was characterised for relevant morpho-phenological traits; a partially replicated experimental design was used. For each quantitative trait, Best Linear Unbiased Predictors and broad-sense heritability were estimated. The screened panel revealed a high level of diversity for most of the measured traits, especially for days to flowering and hundred-seed weight. The same material was also characterised by means of double-digest Restriction-site Associated DNA; a high number of SNP markers were successfully produced. The genotyping allowed understanding the fine genetic structure of the panel. Genetic information was also used to study morpho-phenological traits considering different genetic groups existing within the panel. At the same time, genotypes characterised by favourable traits were identified. The availability of such collection with its extensive characterisation, make this material an excellent resource for common bean improvement.
Fish Communities, Habitat Use, and Human Pressures in the Upper Volta Basin, Burkina Faso, West Africa
Authors: Meulenbroek Paul , Stranzl Sebastian , Oueda Adama , Sendzimir Jan , Mano Komandan , Kabore Idrissa , Ouedraogo Raymond , Melcher Andreas
Published: October 2019

Human pressures and loss of natural fish habitats led to a decline in fish populations in terms of abundances, biodiversity, and average size in sub-Sahelian Burkina Faso. Little knowledge exists about fish assemblages regarding their composition, their habitat preferences, or their sensitivity to or tolerance of human pressures. This research provides the first data-driven basis for sustainably managing fish and associated aquatic and terrestrial habitats. Surveys in four different regions sampled 18,000 specimens from 69 species during the dry season. Fish communities, available abiotic habitat conditions, habitat use, and human pressures were assessed and analyzed. Fish communities cluster into four distinct types, each dominated by either Cichlidae, Clariidae, Cyprinidae, or Alestidae and accompanied by specific other families and genera of fish. Habitat preferences of four key species (Labeo coubie, Bagrus bajad, Chelaethiops bibie, and Lates niloticus) were linked to ecological habitat conditions. Results show that physical parameters influence fish community composition and abundances and, when indexed according to pressure type, are linked to responses in fish metrics. Relative abundance either dropped (Mormyridae) or increased (Cichlidae, Cyprinidae) with rising pressure intensity, and some sentinel taxa (Auchenoglanis, Hydrocynus) were only found in low-pressure sites. The outcomes of this study provide basic knowledge of habitat availability, habitat use by fish, species associations, and human pressures and therefore provide the basis for effective conservation and management of fish populations.
Natural Protected Areas as Special Sentinels of Littering on Coastal Dune Vegetation
Authors: de Francesco Maria Carla , Carranza Maria Laura , Varricchione Marco , Tozzi Francesco Pio , Stanisci Angela
Published: October 2019

Beach litter threatens coastal dunes integrity across the world. European countries are committed to improving the environmental status of the marine and coastal environment by 2020, and to do this, they need to reduce the gap of knowledge about litter accumulation patterns in coastal environments. We analyzed the distribution pattern of waste, differentiated by material and origin, in the coastal dune vegetation mosaic along protected natural areas in the Adriatic seashore (central Italy). Litter data were collected following a random stratified procedure. We registered litter occurrence on 180 (2 × 2 m) sampling plots randomly distributed in the different habitats of European conservation concern mapped for the analyzed protected areas. Litter was classified by origin and material, and their abundance on different habitats was explored by multivariate ordination techniques and tested by nonparametric ANOVA followed by Mann-Whitney pairwise post-hoc tests. Most of the plots included at least one waste element being plastic. Plastic was the most abundant material, and fishing and touristic the most polluting activities. Waste distribution varies across coastal dune vegetation types and involves the back dune zone too. Our results stress the need for (a) specific cleaning tasks able to preserve the ecological value of coastal dune habitats and (b) actions aimed at preventing litter production and accumulation.