bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Baduanjin practice on emotional, attention and cognitive function in acupuncturists: protocol for a clinical randomized controlled neuroimaging trial
Authors: Luo Weiming , Zhou Jun , Zhang Xinyue , Teng Yuke , Tao Siyuan , Chen Nuo , Tong Dan , Su Peiling , Ying Kaijie , Li Zheng jie
Published: April 2024

Background

In Chinese medicine, the mental focus and emotional stability of acupuncturists are key to optimal clinical outcomes. Many renowned acupuncturists utilize Traditional Chinese Qigong practices to enhance their concentration and emotional regulation abilities. Nevertheless, the existing literature lacks comprehensive evidence addressing this matter.

Methods

This study will enroll 99 acupuncturists and randomly allocate them to one of three groups: Baduanjin, aerobic exercise, or a waiting-list control. The Baduanjin group will undertake 24 weeks of training, with three one-hour sessions weekly. The aerobic group will engage in brisk walking for the same duration and frequency. The control group will not receive any specific training. Assessments of emotion regulation, attention, cognitive functions, finger sensation, and athletic ability will be conducted at baseline (−1 week), mid-intervention (12 weeks), and post-intervention (24 weeks). Additionally, 20 participants from each group will undergo fMRI scans before and after the intervention to explore brain functional and structural changes relating to emotion, attention, cognition, motor skills, and sensory perception.

Discussion

This study aims to contribute valuable insights into the effectiveness of Qigong practice, specifically Baduanjin, in enhancing emotional regulation, attention, and cognitive functions in acupuncturists and to investigate the neuroimaging mechanisms behind these effects.

Ethics and dissemination

Approved by the Sichuan Regional Ethics Review Committee on Traditional Chinese Medicine (No. 2023KL − 118) and adhering to the Declaration of Helsinki. Results will be shared through policy briefs, workshops, peer-reviewed journals, and conferences.

Clinical trial registrationwww.chictr.org.cn, ChiCTR2300076447.
Career construction theory: tools, interventions, and future trends
Authors: Wang Danqi , Li Yanling
Published: April 2024

With the emergence of the borderless career era in the 21st century, career coaching has experienced a change from career guidance and career education to career counseling. Career construction theory has been widely used in career counseling and has substantial application value. Introducing career construct theory’s assessment tools and intervention strategies is necessary and meaningful. In this mini-review, the qualitative assessment tools and intervention approaches of career construct theory are introduced and analyzed; the qualitative assessment tools include the Career Construction Interview and “My Career Story” workbook, and the intervention approaches include the Computer-Assisted Career Counseling System, workshops, group counseling, and individual counseling. Finally, future research directions are proposed, including an analysis of what kinds of career construction interventions are most effective for which groups and under what conditions, career intervention in the digital age, and the standardization of assessment tools. The novelty of this paper lies in the fact that it purposefully proposes future directions for career construction theory from the perspectives of assessment tools and intervention approaches and that research on the assessment tools and intervention approaches of career construction theory still needs further attention.
Application value of the automated machine learning model based on modified CT index combined with serological indices in the early prediction of lung cancer
Authors: Meng Leyuan , Zhu Ping , Xia Kaijian
Published: April 2024

Background and objective

Accurately predicting the extent of lung tumor infiltration is crucial for improving patient survival and cure rates. This study aims to evaluate the application value of an improved CT index combined with serum biomarkers, obtained through an artificial intelligence recognition system analyzing CT features of pulmonary nodules, in early prediction of lung cancer infiltration using machine learning models.

Patients and methods

A retrospective analysis was conducted on clinical data of 803 patients hospitalized for lung cancer treatment from January 2020 to December 2023 at two hospitals: Hospital 1 (Affiliated Changshu Hospital of Soochow University) and Hospital 2 (Nantong Eighth People’s Hospital). Data from Hospital 1 were used for internal training, while data from Hospital 2 were used for external validation. Five algorithms, including traditional logistic regression (LR) and machine learning techniques (generalized linear models [GLM], random forest [RF], gradient boosting machine [GBM], deep neural network [DL], and naive Bayes [NB]), were employed to construct models predicting early lung cancer infiltration and were analyzed. The models were comprehensively evaluated through receiver operating characteristic curve (AUC) analysis based on LR, calibration curves, decision curve analysis (DCA), as well as global and individual interpretative analyses using variable feature importance and SHapley additive explanations (SHAP) plots.

Results

A total of 560 patients were used for model development in the training dataset, while a dataset comprising 243 patients was used for external validation. The GBM model exhibited the best performance among the five algorithms, with AUCs of 0.931 and 0.99 in the validation and test sets, respectively, and accuracies of 0.857 and 0.955 in the validation and test groups, respectively, outperforming other models. Additionally, the study found that nodule diameter and average CT value were the most significant features for predicting lung cancer infiltration using machine learning models.

Conclusion

The GBM model established in this study can effectively predict the risk of infiltration in early-stage lung cancer patients, thereby improving the accuracy of lung cancer screening and facilitating timely intervention for infiltrative lung cancer patients by clinicians, leading to early diagnosis and treatment of lung cancer, and ultimately reducing lung cancer-related mortality.
Short-term association of particulate matter and cardiovascular disease mortality in Shanghai, China between 2003 and 2020
Authors: Fang Bo , Wei Jing , Chen Lei , Jin Shan , Li Qi , Cai Renzhi , Qian Naisi , Gu Zhen , Chen Lei , Santon Romain , Wang Chunfang , Song Weimin
Published: April 2024

Objective

Evidence regarding the effects of particulate matter (PM) pollutants on cardiovascular disease (CVD) mortality remains limited in Shanghai, China. Our objective was to thoroughly evaluate associations between PM pollutants and CVD mortality.

Methods

Daily data on CVD mortality, PM (PM10 and PM2.5) pollutants, and meteorological variables in Shanghai, China were gathered from 2003 to 2020. We utilized a time-series design with the generalized additive model to assess associations between PM pollutants and CVD mortality. Additionally, we conducted stratified analyses based on sex, age, education, and seasons using the same model.

Results

We found that PM pollutants had a significant association with CVD mortality during the study period. Specifically, there was a 0.29% (95%CI: 0.14, 0.44) increase in CVD mortality for every 10 μg/m3 rise in a 2-day average (lag01) concentration of PM10. A 0.28% (95% CI: 0.07, 0.49) increase in CVD mortality was associated with every 10 μg/m3 rise in PM2.5 concentration at lag01. Overall, the estimated effects of PM10 and PM2.5 were larger in the warm period compared with the cold period. Furthermore, males and the older adult exhibited greater susceptibility to PM10 and PM2.5 exposure, and individuals with lower education levels experienced more significant effects from PM10 and PM2.5 than those with higher education levels.

Conclusion

Our findings suggested that PM pollutants have a substantial impact on increasing CVD mortality in Shanghai, China. Moreover, the impacts of air pollution on health may be altered by factors such as season, sex, age, and educational levels.
Interactive network visualization of opioid crisis research: a tool for reinforcing data linkage skills for public health policy researchers
Authors: Scrivner Olga , Nguyen Thuy , Ginda Michael , Simon Kosali , Börner Katy
Published: April 2024

Background

Public health policy researchers face a persistent challenge in identifying and integrating relevant data, particularly in the context of the U.S. opioid crisis, where a comprehensive approach is crucial.

Purpose

To meet this new workforce demand health policy and health economics programs are increasingly introducing data analysis and data visualization skills. Such skills facilitate data integration and discovery by linking multiple resources. Common linking strategies include individual or aggregate level linking (e.g., patient identifiers) in primary clinical data and conceptual linking (e.g., healthcare workforce, state funding, burnout rates) in secondary data. Often, the combination of primary and secondary datasets is sought, requiring additional skills, for example, understanding metadata and constructing interlinkages.

Methods

To help improve those skills, we developed a 2-step process using a scoping method to discover data and network visualization to interlink metadata. Results: We show how these new skills enable the discovery of relationships among data sources pertinent to public policy research related to the opioid overdose crisis and facilitate inquiry across heterogeneous data resources. In addition, our interactive network visualization introduces (1) a conceptual approach, drawing from recent systematic review studies and linked by the publications, and (2) an aggregate approach, constructed using publicly available datasets and linked through crosswalks.

Conclusions

These novel metadata visualization techniques can be used as a teaching tool or a discovery method and can also be extended to other public policy domains.
The effectiveness of dual regulation and synergistic governance of market-incentivized carbon reduction policies and public environmental supervision: a study based on the sustainable development performance of listed companies in China
Authors: Chen Jiahe , Yu Wenhao
Published: April 2024

Introduction

In the current background of global economic slowdown, the traditional reliance on one regulatory instrument or the unilateral consideration of the effectiveness of one regulatory policy in policy formulation is no longer sufficient to cope with the increasingly complex contradictions between environmental protection and economic development. In the construction of a modernized environmental governance system, it has become an inevitable choice to achieve synergy between various environmental regulations. In China, the citizens' environmental supervision campaign that gradually emerged in 2006 and the local carbon trading pilots that started in 2013, as typical representatives of informal and formal environmental regulation respectively, provide vivid and realistic materials for our study.

Methods

Using econometric models and microdata from listed Chinese firms from 2009 to 2020, we analyze the profound logic and internal mechanism by which this synergistic governance effect of environmental regulation affects the economic society and the development pattern of firms.

Results and discussion

The study found that: (1) the synergistic effect of the carbon trading policy and citizens' environmental supervision can significantly promote the transition of enterprises to a sustainable development model, especially paying attention to the role of citizens' environmental supervision in this process. (2) The regional development level, cost transfer capability, and political connection can make the synergy of the two environmental regulations vary across firms. (3) The synergistic effect of environmental regulation promotes the behavior of enterprises in line with the requirements of sustainable development by influencing enterprises' R&D and innovation investment, resource allocation efficiency, and sustainable development awareness. (4) A favorable regional rule of law environment and moderate media attention can effectively increase the intensity of citizens' environmental supervision, and at the same time strengthen the effectiveness of synergistic governance of environmental regulation in the transformation and development of enterprises.
Pancreatic duct occlusion with polychloroprene-based glue for the management of postoperative pancreatic fistula after pancreatoduodenectomy—an outdated approach?
Authors: Yaqub Sheraz , Røsok Bård , Gladhaug Ivar Prydz , Labori Knut Jørgen
Published: April 2024

Background

Managing postoperative pancreatic fistula (POPF) presents a formidable challenge after pancreatoduodenectomy. Some centers consider pancreatic duct occlusion (PDO) in reoperations following pancreatoduodenectomy as a pancreas-preserving procedure, aiming to control a severe POPF. The aim of the current study was to evaluate the short- and long-term outcomes of employing PDO for the management of the pancreatic stump during relaparotomy for POPF subsequent to pancreatoduodenectomy.

Methods

Retrospective review of consecutive patients at Oslo University Hospital undergoing pancreatoduodenectomy and PDO during relaparotomy. Pancreatic stump management during relaparotomy consisted of occlusion of the main pancreatic duct with polychloroprene Faxan-Latex, after resecting the dehiscent jejunal loop previously constituting the pancreaticojejunostomy.

Results

Between July 2005 and September 2015, 826 pancreatoduodenectomies were performed. Overall reoperation rate was 13.2% (n = 109). POPF grade B/C developed in 113 (13.7%) patients. PDO during relaparotomy was performed in 17 (2.1%) patients, whereas completion pancreatectomy was performed in 22 (2.7%) patients. Thirteen (76%) of the 17 patients had a persistent POPF after PDO, and the time from PDO until removal of the last abdominal drain was median 35 days. Of the PDO patients, 13 (76%) patients required further drainage procedures (n = 12) or an additional reoperation (n = 1). In-hospital mortality occurred in one patient (5.9%). Five (29%) patients developed new-onset diabetes mellitus, and 16 (94%) patients acquired exocrine pancreatic insufficiency.

Conclusions

PDO is a safe and feasible approach for managing severe POPF during reoperation following pancreatoduodenectomy. A significant proportion of patients experience persistent POPF post-procedure, necessitating supplementary drainage interventions. The findings suggest that it is advisable to explore alternative pancreas-preserving methods before opting for PDO in the management of POPF subsequent to pancreatoduodenectomy.
Microenvironment, systemic inflammatory response and tumor markers considering consensus molecular subtypes of colorectal cancer
Authors: Jakab Anna , Patai Árpád V. , Darvas Mónika , Tormássi-Bély Karolina , Micsik Tamás
Published: April 2024

Introduction: Colorectal carcinomas (CRC) are one of the most frequent malignancies worldwide. Based on gene expression profile analysis, CRCs can be classified into four distinct subtypes also known as the consensus molecular subtypes (CMS), which predict biological behaviour. Besides CMS, several other aspects of tumor microenvironment (TME) and systemic inflammatory response (SIR) influence the outcome of CRC patients. TME and inflammation have important role in the immune (CMS1) and mesenchymal (CMS4) subtypes, however, the relationship between these and systemic inflammation has not been assessed yet. Our objective was to evaluate the connection between CMS, TME and SIR, and to analyze the correlation between these markers and routinely used tumor markers, such as CEA (Carcinoembryonic Antigen) and CA19-9 (Carbohydrate Antigen 19-9).

Methods: FFPE (Formalin Fixed Paraffin Embedded) samples of 185 CRC patients were collected. TME was described using tumor-stroma ratio (TSR), Klintrup-Makinen (KM) grade, and Glasgow Microenvironment Score (GMS). CMS classification was performed on tissue microarray using MLH1, PMS2, MSH2 and MSH6, and pan-cytokeratin, CDX2, FRMD6, HTR2B and ZEB1 immunohistochemical stains. Pre-operative tumor marker levels and inflammatory markers [C-reactive protein - CRP, albumin, absolute neutrophil count (ANC), absolute lymphocyte count (ALC), absolute platelet count (APC)] and patient history were retrieved using MedSolution database.

Results: Amongst TME-markers, TSR correlated most consistently with adverse clinicopathological features (p < 0.001) and overall survival (p < 0.001). Elevated CRP and modified Glasgow Prognostic Score (mGPS) were associated with worse outcome and aggressive phenotype, similarly to tumor markers CEA and CA19-9. Stroma–Tumor Marker score (STM score), a new combined score of CA19-9 and TSR delivered the second best prognostication after mGPS. Furthermore, CMS4 showed association with TSR and several laboratory markers (albumin and platelet derived factors), but not with other SIR descriptors. CMS did not show any association with CEA and CA19-9 tumor markers.

Conclusion: More routinely available TME, SIR and tumor markers alone and in combination deliver reliable prognostic data for choosing the patients with higher risk for propagation. CMS4 is linked with high TSR and poor prognosis, but in overall, CMS-classification showed only limited effect on SIR- and tumor-markers.
Resource Allocation of Cooperative Alternatives Using the Analytic Hierarchy Process and Analytic Network Process with Shapley Values
Authors: Huang Jih-Jeng , Chen Chin-Yi
Published: April 2024

Cooperative alternatives need complex multi-criteria decision-making (MCDM) consideration, especially in resource allocation, where the alternatives exhibit interdependent relationships. Traditional MCDM methods like the Analytic Hierarchy Process (AHP) and Analytic Network Process (ANP) often overlook the synergistic potential of cooperative alternatives. This study introduces a novel method integrating AHP/ANP with Shapley values, specifically designed to address this gap by evaluating alternatives on individual merits and their contributions within coalitions. Our methodology begins with defining problem structures and applying AHP/ANP to determine the criteria weights and alternatives’ scores. Subsequently, we compute Shapley values based on coalition values, synthesizing these findings to inform resource allocation decisions more equitably. A numerical example of budget allocation illustrates the method’s efficacy, revealing significant insights into resource distribution when cooperative dynamics are considered. Our results demonstrate the proposed method’s superiority in capturing the nuanced interplay between criteria and alternatives, leading to more informed urban planning decisions. This approach marks a significant advancement in MCDM, offering a comprehensive framework that incorporates both the analytical rigor of AHP/ANP and the equitable considerations of cooperative game theory through Shapley values.
Cellular and Molecular Effects of Microgravity on the Immune System: A Focus on Bioactive Lipids
Authors: Fava Marina , De Dominicis Noemi , Forte Giulia , Bari Monica , Leuti Alessandro , Maccarrone Mauro
Published: April 2024

Microgravity is one of the main stressors that astronauts are exposed to during space missions. This condition has been linked to many disorders, including those that feature dysfunctional immune homeostasis and inflammatory damage. Over the past 30 years, a significant body of work has been gathered connecting weightlessness—either authentic or simulated—to an inefficient reaction to pathogens, dysfunctional production of cytokines and impaired survival of immune cells. These processes are also orchestrated by a plethora of bioactive lipids, produced by virtually all cells involved in immune events, which control the induction, magnitude, outcome, compartmentalization and trafficking of immunocytes during the response to injury. Despite their crucial importance in inflammation and its modulation, however, data concerning the role of bioactive lipids in microgravity-induced immune dysfunctions are surprisingly scarce, both in quantity and in variety, and the vast majority of it focuses on two lipid classes, namely eicosanoids and endocannabinoids. The present review aims to outline the accumulated knowledge addressing the effects elicited by microgravity—both simulated and authentic—on the metabolism and signaling of these two prominent lipid groups in the context of immune and inflammatory homeostasis.
Role of Vanadium in Thermal and Hydrothermal Aging of a Commercial V2O5-WO3/TiO2 Monolith for Selective Catalytic Reduction of NOx: A Case Study
Authors: Consentino Luca , Pantaleo Giuseppe , La Parola Valeria , La Greca Eleonora , Gallì Nunzio , Marcì Giuseppe , Fiorenza Roberto , Scirè Salvatore , Liotta Leonarda Francesca
Published: April 2024

In recent years, increased attention to air pollutants such as NOx has led the scientific community to focus meaningfully on developing strategies for NOx reduction. Selective catalytic reduction of NOx by ammonia (NO SCR by NH3) is currently the main method to remove NOx from diesel engine exhaust emissions. The catalysts with typical V2O5-WO3/TiO2 (VWTi) composition are widely used in NH3-SCR for their high NOx conversion activity, low cost, and robustness, especially concerning sulfur poisoning. However, in real diesel engine working conditions, the thermal and hydrothermal aging of catalysts can occur after several hours of operation at high temperature, affecting the catalytic performance. In this study, the stability of a commercial VWTi monolith, self-supported and containing glass fibers and bentonite in its matrix, was investigated as a case study. In laboratory conditions, NO SCR tests were performed for 50 h in the range of 150 to 350 °C. Subsequently, the VWTi monolith was thermally and hydrothermally aged at 600 °C for 6 h. The thermal aging increased the NOx conversion, especially at low temperature (<250 °C), while the hydrothermal aging did not affect the SCR. The differences in NOx conversion before and after aging were associated with the change in vanadium and tungsten oxide surface coverage and with the reduction in the surface area of catalysts. In order to correlate the change in SCR activity with the modifications occurring after aging processes, the monolithic samples were characterized by several techniques, namely XRD, SSA and pore analysis, TPR, XPS, Raman, TGA and SEM/EDX.
Foam Cells Analysis from Retrieved Stroke Clot for the Identification of Atherothrombotic Etiology
Authors: Giammello Fabrizio , Ciacciarelli Antonio , Cosenza Domenico , Galletta Santi , Barresi Valeria , La Spina Paolino , Fazio Maria Carolina , De Caro Jolanda , Cotroneo Masina , Dell’Aera Cristina , Grillo Francesco , Ammendola Serena , Tessitore Agostino , Vinci Sergio Lucio , Musolino Rosa Fortunata , Casella Carmela , Toscano Antonio
Published: April 2024

Background: In atherothrombotic acute ischemic stroke (AIS), when the atheroma breaks down, the clot can incorporate foam cells (FCs). The identification of the correct etiology is paramount for secondary stroke prevention. This study aims to evaluate the presence of the FC in the arterial clot, and to determine whether patients with FCs and patients without FCs (NFCs) had different cerebrovascular risk factors, haemato-chemical parameters, and atherosclerotic disease incidence, in order to predict the etiological diagnosis. Methods: We collected 100 clots retrieved by mechanical thrombectomy from 495 consecutive AIS patients with large vessel occlusion. An expert pathologist evaluated the FC presence by histological examination stained with hematoxylin and eosin. Results: We observed FCs in 29/100 (29%) of retrieved clots and divided the patients into two groups, with/without FCs. The two groups had similar clinical and laboratory features, with a discrepancy between the FC presence in the clot and the clinical etiological diagnosis, even if not statistically significant. Conclusions: Our study showed the presence of FCs in approximately one-third of the retrieved clots, but the identification of the clot that presumably comes from the atheromatous plaque rupture tended to disagree with the clinical diagnosis. Future studies may reveal their potential to disclose clot origin or specific patient characteristics, guiding treatment options.
Liver-Directed Locoregional Therapies for Neuroendocrine Liver Metastases: Recent Advances and Management
Authors: Criss Cody R. , Makary Mina S.
Published: April 2024

Neuroendocrine tumors (NETs) are a heterogeneous class of cancers, predominately occurring in the gastroenteropancreatic system, which pose a growing health concern with a significant rise in incidence over the past four decades. Emerging from neuroendocrine cells, these tumors often elicit paraneoplastic syndromes such as carcinoid syndrome, which can manifest as a constellation of symptoms significantly impacting patients’ quality of life. The prognosis of NETs is influenced by their tendency for metastasis, especially in cases involving the liver, where the estimated 5-year survival is between 20 and 40%. Although surgical resection remains the preferred curative option, challenges emerge in cases of neuroendocrine tumors with liver metastasis (NELM) with multifocal lobar involvement, and many patients may not meet the criteria for surgery. Thus, minimally invasive and non-surgical treatments, such as locoregional therapies, have surfaced. Overall, these approaches aim to prioritize symptom relief and aid in overall tumor control. This review examines locoregional therapies, encompassing catheter-driven procedures, ablative techniques, and radioembolization therapies. These interventions play a pivotal role in enhancing progression-free survival and managing hormonal symptoms, contributing to the dynamic landscape of evolving NELM treatment. This review meticulously explores each modality, presenting the current state of the literature on their utilization and efficacy in addressing NELM.
Lossless and Near-Lossless Compression Algorithms for Remotely Sensed Hyperspectral Images
Authors: Altamimi Amal , Ben Youssef Belgacem
Published: April 2024

Rapid and continuous advancements in remote sensing technology have resulted in finer resolutions and higher acquisition rates of hyperspectral images (HSIs). These developments have triggered a need for new processing techniques brought about by the confined power and constrained hardware resources aboard satellites. This article proposes two novel lossless and near-lossless compression methods, employing our recent seed generation and quadrature-based square rooting algorithms, respectively. The main advantage of the former method lies in its acceptable complexity utilizing simple arithmetic operations, making it suitable for real-time onboard compression. In addition, this near-lossless compressor could be incorporated for hard-to-compress images offering a stabilized reduction at nearly 40% with a maximum relative error of 0.33 and a maximum absolute error of 30. Our results also show that a lossless compression performance, in terms of compression ratio, of up to 2.6 is achieved when testing with hyperspectral images from the Corpus dataset. Further, an improvement in the compression rate over the state-of-the-art k2-raster technique is realized for most of these HSIs by all four variations of our proposed lossless compression method. In particular, a data reduction enhancement of up to 29.89% is realized when comparing their respective geometric mean values.
Geochemical Properties and Gas-Bearing Analysis of Lower Cambrian Black Shale in Western Hunan Province
Authors: Zhang Kaixun , Tang Xiaoyin , Liu Xiaoqiang , Zhao Zisheng , Li Meijun
Published: April 2024

Western Hunan province and its surrounding areas are significant targets for shale gas exploration and development in southern China, where the black shale of the lower Cambrian Niutitang Formation and Wunitang Formation is extensively distributed. Geochemical analysis was conducted on the lower Cambrian black shale from a new exploration well of XAD1 located at the southeast margin of the Yangtze paraplatform, followed by a discussion on gas-bearing properties using molecular dynamics simulation. The geochemical characteristics indicate that the black shale in well XAD1 was primarily deposited in a strongly reducing marine environment, with organic matter predominantly composed of type I kerogen derived from algae. Currently, it has reached a stage of high to over maturity with limited potential for liquid hydrocarbon generation. The recovery of the original hydrocarbon generation potential shows that they are excellent source rocks and have completed the main hydrocarbon generation evolution. Despite the favorable conditions for shale gas formation observed in well XAD1, the low measured gas content within the Niutitang Formation suggests that other geological factors may have contributed to a substantial loss of shale gas. Gas adsorption simulation reveals that the maximum methane adsorption capacity (15.77 m3/t) was achieved by Niutitang shale during the late Silurian period when there was an abundant source of natural gas without any influence from CO2, H2O or other molecules. However, due to a lack of natural gas replenishment and subsequent tectonic uplift and subsidence causing variations in temperature and pressure, the methane adsorption capacity gradually decreased (to 6.56 m3/t). Furthermore, water occurrence within the shale reservoir further reduced the methane adsorption capacity (below 2 m3/t), while tectonic activities exacerbated the loss of shale gas potential within this study area. The findings indicate that the dynamic alteration of gas-bearing properties in shale reservoirs due to tectonic movements is a crucial factor influencing the success rate of shale gas exploration in the study area, provided that there are sufficient gas resources and superior reservoir conditions.
Research on the Performance Improvement Method for Lithium-Ion Battery in High-Power Application Scenarios
Authors: Zhou Pengfei , Zhu Liying , Fu Dawei , Du Jianguo , Zhao Xinze , Sun Bingxiang
Published: April 2024

With the development of technology, high-power lithium-ion batteries are increasingly moving towards high-speed discharge, long-term continuous output, instantaneous high-rate discharge, and miniaturization, and are being gradually developed towards the fields of electric tools, port machinery and robotics. Improving the power performance of batteries can be achieved from multiple dimensions, such as electrochemical systems and battery design. In order to improve the power performance of lithium-ion batteries, this paper proposes design methods from the perspective of electrochemical systems, which include increasing the high-rate discharge capacity and low impedance of the battery. This article also studies the preparation of high-power lithium-ion batteries. This article aims to improve the rate performance of batteries by studying high-performance cathode materials, excellent conductive networks, and high-performance electrolytes. This article successfully screened high-performance cathode materials by comparing the effects of different particle sizes of cathode materials on electrode conductivity and battery internal resistance. By comparing the effects of electrolyte additives under pulse cycling, high-quality electrolyte additive materials were selected. By comparing the effects of different types, contents, and ratios of conductive agents on electrode conductivity, battery internal resistance, high-quality conductive agents, and appropriate ratios were selected. Finally, a 10 Ah cylindrical high-power lithium-ion battery with a specific energy of 110 Wh/kg, pulse discharge specific power of 11.3 kW/kg, an AC internal resistance of ≤0.7 m Ω, a 10C full capacity discharge cycle of over 1700, a 30C full capacity discharge cycle of over 500, and a continuous discharge capacity of 10C–30C, and a pulse discharge capacity of over 100C was prepared.
Calcium Pyrophosphate and Basic Calcium Phosphate Crystal Arthritis: 2023 in Review
Authors: Latourte Augustin , Ea Hang-Korng , Richette Pascal
Published: April 2024

Calcium-containing crystal deposition diseases are extremely common in rheumatology. However, they are under-explored compared to gout or other inflammatory rheumatic diseases. Major advances have been made in 2023 that will undoubtedly stimulate and facilitate research in the field of calcium pyrophosphate (CPP) deposition disease (CPPD): the ACR/EULAR classification criteria for CPPD and a semi-quantitative OMERACT score for ultrasound assessment of the extent of CPP deposition have been validated and published. A large randomized controlled trial compared the efficacy and safety of colchicine and prednisone in acute CPP arthritis. Preclinical studies have elucidated the pro-inflammatory and anti-catabolic effects of basic calcium phosphate (BCP) crystals on mononuclear cells and chondrocytes. The association between osteoarthritis (OA) and IA calcifications has been the subject of several epidemiological publications, suggesting that calcium crystals are associated with a greater risk of progression of knee OA. Research in the field of calcium crystal deposition diseases is active: the areas of investigation for the coming years are broad and promising.
Emergency Actions for the Documentation, Stabilization, and Consolidation of the Early Bronze Age Fortifications at Khirbat Iskandar, Jordan
Authors: D’Andrea Marta , Richard Suzanne , Long Jesse C. , Sciorilli Franco
Published: April 2024

Khirbat Iskandar is an Early Bronze Age (ca. 3800-1950 BCE) mound in the Madaba Governorate of Jordan. Until a decade ago, it was better known as a key site for the non-urban Early Bronze IV period (ca. 2500-1950 BCE), but is increasingly emerging as a signature site for the urban Early Bronze II-III period (ca. 3050-2500 BCE). The contour of the tall site is shaped by the presence of buried fortifications that were investigated in the north-western sector of the mound, where a long sequence of rebuilds was recognized, but were exposed to and impacted by modern construction activities along the southern and south-eastern areas prior to being recorded. There, due to erosion and weathering, the stone fortifications dating to the first half of the Early Bronze III (ca. 2850-2650 BCE) fell down. In this paper, we report on our strategies to assess threats, document damages, sustainably stabilize, and consolidate the collapsed ancient fortifications in the 2023 field season at Khirbat Iskandar. At the same time, we discuss the conceptual/methodological and practical challenges of identifying best practices in the conservation and preservation of antiquities that collapsed prior to being excavated. We conclude with some thoughts on how to build on these actions to efficiently present the archaeology and cultural heritage at protohistoric sites, like Khirbat Iskandar, and to make it accessible for the local and the international communities.
Introducing ‘Ngaruroro’, a New Model for Understanding Māori Wellbeing
Authors: Johnson Finley Ngarangi , Wehi Priscilla , Neha Tia , Ross Mike , Thompson Veronica , Tibble Stephanie , Tassell-Matamua Natasha , Shedlock Kevin , Fox Ririwai , Penman Zachary , Ritchie Tyler , Winter Taylor , Arahanga-Doyle Hitaua , Jose Paul E.
Published: April 2024

Indigenous peoples around the world are revitalising their ancestral beliefs, practices, and languages, including traditional understandings of health and wellbeing. In the Aotearoa (New Zealand) context, a number of ground-breaking Māori health- and wellbeing-related models have emerged, each with their own scope and applications. We sought in our qualitative studies to explore and identify several key sources of wellbeing for Māori individuals. Nine interviews were conducted with members of Māori communities to identify key themes of Māori wellbeing. We performed a Reflexive Thematic Analysis on these data and then conducted a further fifteen interviews to revise, refine, and reposition the previously generated themes. The Ngaruroro model describes wellbeing as the embodied and active process of being well in relation with one’s (1) here tāngata (social and familial ties), (2) te taiao (the environment), and (3) taonga tuku iho (cultural treasures) while doing what one can to make lifestyle choices that are conducive to the health of one’s (4) tinana (body) and (5) wairua (spirit) while cultivating a balanced (6) ngākau (inner-system), fulfilling (7) matea (core needs) and exercising your (8) mana (authority). These themes illustrate that Māori wellbeing is dynamic, interconnected, and holistic.
Amyloid-Forming Corpora Amylacea and Spheroid-Type Amyloid Deposition: Comprehensive Analysis Using Immunohistochemistry, Proteomics, and a Literature Review
Authors: Ichimata Shojiro , Hata Yukiko , Yoshinaga Tsuneaki , Katoh Nagaaki , Kametani Fuyuki , Yazaki Masahide , Sekijima Yoshiki , Nishida Naoki
Published: April 2024

This study aimed to elucidate the similarities and differences between amyloid-forming corpora amylacea (CA) in the prostate and lung, examine the nature of CAs in cystic tumors of the atrioventricular node (CTAVN), and clarify the distinctions between amyloid-forming CA and spheroid-type amyloid deposition. We conducted proteomics analyses using liquid chromatography–tandem mass spectrometry with laser microdissection and immunohistochemistry to validate the characteristics of CAs in the lung and prostate. Our findings revealed that the CAs in these organs primarily consisted of common proteins (β2-microglobulin and lysozyme) and locally produced proteins. Moreover, we observed a discrepancy between the histopathological and proteomic analysis results in CTAVN-associated CAs. In addition, while the histopathological appearance of the amyloid-forming CAs and spheroid-type amyloid deposits were nearly identical, the latter deposition lacked β2-microglobulin and lysozyme and exhibited evident destruction of the surrounding tissue. A literature review further supported these findings. These results suggest that amyloid-forming CAs in the lung and prostate are formed through a shared mechanism, serving as waste containers (wasteosomes) and/or storage for excess proteins (functional amyloids). In contrast, we hypothesize that while amyloid-forming CA and spheroid-type amyloid deposits are formed, in part, through common mechanisms, the latter are pathological.
Contrast Agents Based on Human Serum Albumin and Nitroxides for 1H-MRI and Overhauser-Enhanced MRI
Authors: Mitin Dmitry , Bullinger Friedemann , Dobrynin Sergey , Engelmann Jörn , Scheffler Klaus , Kolokolov Mikhail , Krumkacheva Olesya , Buckenmaier Kai , Kirilyuk Igor , Chubarov Alexey
Published: April 2024

In cancer diagnostics, magnetic resonance imaging (MRI) uses contrast agents to enhance the distinction between the target tissue and background. Several promising approaches have been developed to increase MRI sensitivity, one of which is Overhauser dynamic nuclear polarization (ODNP)-enhanced MRI (OMRI). In this study, a macromolecular construct based on human serum albumin and nitroxyl radicals (HSA-NIT) was developed using a new synthesis method that significantly increased the modification to 21 nitroxide residues per protein. This was confirmed by electron paramagnetic resonance (EPR) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight (MALDI ToF) mass spectrometry. Gel electrophoresis and circular dichroism showed no significant changes in the structure of HSA-NITs, and no oligomers were formed during modification. The cytotoxicity of HSA-NITs was comparable to that of native albumin. HSA-NITs were evaluated as potential “metal-free” organic radical relaxation-based contrast agents for 1H-MRI and as hyperpolarizing contrast agents for OMRI. Relaxivities (longitudinal and transversal relaxation rates r1 and r2) for HSA-NITs were measured at different magnetic field strengths (1.88, 3, 7, and 14 T). Phantoms were used to demonstrate the potential use of HSA-NIT as a T1- and T2-weighted relaxation-based contrast agent at 3 T and 14 T. The efficacy of 1H Overhauser dynamic nuclear polarization (ODNP) in liquids at an ultralow magnetic field (ULF, B0 = 92 ± 0.8 μT) was investigated for HSA-NIT conjugates. The HSA-NITs themselves did not show ODNP enhancement; however, under the proteolysis conditions simulating cancer tissue, HSA-NIT conjugates were cleaved into lower-molecular-weight (MW) protein fragments that activate ODNP capabilities, resulting in a maximum achievable enhancement |Emax| of 40–50 and a radiofrequency power required to achieve half of Emax, P1/2, of 21–27 W. The HSA-NIT with a higher degree of modification released increased the number of spin probes upon biodegradation, which significantly enhanced the Overhauser effect. Thus, HSA-NITs may represent a new class of MRI relaxation-based contrast agents as well as novel cleavable conjugates for use as hyperpolarizing contrast agents (HCAs) in OMRI.
Thermosensitive TRP Channels Are Functionally Expressed and Influence the Lipogenesis in Human Meibomian Gland Cells
Authors: Keller Melina , Mergler Stefan , Li Aruna , Zahn Ingrid , Paulsen Friedrich , Garreis Fabian
Published: April 2024

While the involvement of thermosensitive transient receptor potential channels (TRPs) in dry eye disease (DED) has been known for years, their expression in the meibomian gland (MG) has never been investigated. This study aims to show their expression and involvement in the lipogenesis of the MG, providing a possible new drug target in the treatment of DED. Our RT-PCR, Western blot and immunofluorescence analysis showed the expression of TRPV1, TRPV3, TRPV4 and TRPM8 in the MG at the gene and the protein level. RT-PCR also showed gene expression of TRPV2 but not TRPA1. Calcium imaging and planar patch-clamping performed on an immortalized human meibomian gland epithelial cell line (hMGECs) demonstrated increasing whole-cell currents after the application of capsaicin (TRPV1) or icilin (TRPM8). Decreasing whole-cell currents could be registered after the application of AMG9810 (TRPV1) or AMTB (TRPM8). Oil red O staining on hMGECs showed an increase in lipid expression after TRPV1 activation and a decrease after TRPM8 activation. We conclude that thermo-TRPs are expressed at the gene and the protein level in MGs. Moreover, TRPV1 and TRPM8’s functional expression and their contribution to their lipid expression could be demonstrated. Therefore, TRPs are potential drug targets and their clinical relevance in the therapy of meibomian gland dysfunction requires further investigation.
Complement MASP-1 Modifies Endothelial Wound Healing
Authors: Németh Zsuzsanna , Demeter Flóra , Dobó József , Gál Péter , Cervenak László
Published: April 2024

Endothelial wound-healing processes are fundamental for the maintenance and restoration of the circulatory system and are greatly affected by the factors present in the blood. We have previously shown that the complement protein mannan-binding lectin-associated serine protease-1 (MASP-1) induces the proinflammatory activation of endothelial cells and is able to cooperate with other proinflammatory activators. Our aim was to investigate the combined effect of mechanical wounding and MASP-1 on endothelial cells. Transcriptomic analysis showed that MASP-1 alters the expression of wound-healing-related and angiogenesis-related genes. Both wounding and MASP-1 induced Ca2+ mobilization when applied individually. However, MASP-1-induced Ca2+ mobilization was inhibited when the treatment was preceded by wounding. Mechanical wounding promoted CREB phosphorylation, and the presence of MASP-1 enhanced this effect. Wounding induced ICAM-1 and VCAM-1 expression on endothelial cells, and MASP-1 pretreatment further increased VCAM-1 levels. MASP-1 played a role in the subsequent stages of angiogenesis, facilitating the breakdown of the endothelial capillary network on Matrigel®. Our findings extend our general understanding of endothelial wound healing and highlight the importance of complement MASP-1 activation in wound-healing processes.
Thymic Stromal Lymphopoietin (TSLP) Is Cleaved by Human Mast Cell Tryptase and Chymase
Authors: Canè Luisa , Poto Remo , Palestra Francesco , Iacobucci Ilaria , Pirozzi Marinella , Parashuraman Seetharaman , Ferrara Anne Lise , Illiano Amalia , La Rocca Antonello , Mercadante Edoardo , Pucci Piero , Marone Gianni , Spadaro Giuseppe , Loffredo Stefania , Monti Maria , Varricchi Gilda
Published: April 2024

Thymic stromal lymphopoietin (TSLP), mainly expressed by epithelial cells, plays a central role in asthma. In humans, TSLP exists in two variants: the long form TSLP (lfTSLP) and a shorter TSLP isoform (sfTSLP). Macrophages (HLMs) and mast cells (HLMCs) are in close proximity in the human lung and play key roles in asthma. We evaluated the early proteolytic effects of tryptase and chymase released by HLMCs on TSLP by mass spectrometry. We also investigated whether TSLP and its fragments generated by these enzymes induce angiogenic factor release from HLMs. Mass spectrometry (MS) allowed the identification of TSLP cleavage sites caused by tryptase and chymase. Recombinant human TSLP treated with recombinant tryptase showed the production of 1-97 and 98-132 fragments. Recombinant chymase treatment of TSLP generated two peptides, 1-36 and 37-132. lfTSLP induced the release of VEGF-A, the most potent angiogenic factor, from HLMs. By contrast, the four TSLP fragments generated by tryptase and chymase failed to activate HLMs. Long-term TSLP incubation with furin generated two peptides devoid of activating property on HLMs. These results unveil an intricate interplay between mast cell-derived proteases and TSLP. These findings have potential relevance in understanding novel aspects of asthma pathobiology.