bluuebunny/crossref_metadata_2025 huggingface dataset:
Cognitive Sequelae and Hippocampal Dysfunction in Chronic Kidney Disease following 5/6 Nephrectomy
Authors: Yu Yeon , Kim Seong-Wook , Im Hyuna , Oh Se , Cho Nam-Jun , Park Samel , Park Dae-Kyoon , Kim Duk-Soo , Gil Hyo-Wook
Published: July 2022

Neurological disorders are prevalent in patients with chronic kidney disease (CKD). Vascular factors and uremic toxins are involved with cognitive impairment in CKD. In addition, vascular dementia-induced alterations in the structure and function of the hippocampus can lead to deficits in hippocampal synaptic plasticity and cognitive function. However, regardless of this clinical evidence, the pathophysiology of cognitive impairment in patients with CKD is not fully understood. We used male Sprague Dawley rats and performed 5/6 nephrectomy to observe the changes in behavior, field excitatory postsynaptic potential, and immunostaining of the hippocampus following CKD progression. We measured the hippocampus volume on magnetic resonance imaging scans in the controls (n = 34) and end-stage renal disease (ESRD) hemodialysis patients (n = 42). In four cognition-related behavior assays, including novel object recognition, Y-maze, Barnes maze, and classical contextual fear conditioning, we identified deficits in spatial working memory, learning and memory, and contextual memory, as well as the ability to distinguish familiar and new objects, in the rats with CKD. Immunohistochemical staining of Na+/H+ exchanger1 was increased in the hippocampus of the CKD rat models. We performed double immunofluorescent staining for aquaporin-4 and glial fibrillary acidic protein and then verified the high coexpression in the hippocampus of the CKD rat model. Furthermore, results from recoding of the field excitatory postsynaptic potential (fEPSP) in the hippocampus showed the reduced amplitude and slope of fEPSP in the CKD rats. ESRD patients with cognitive impairment showed a significant decrease in the hippocampus volume compared with ESRD patients without cognitive impairment or the controls. Our findings suggest that uremia resulting from decreased kidney function may cause the destruction of the blood–brain barrier and hippocampus-related cognitive impairment in CKD.
Structural Analysis and Design of Sustainable Cross-Laminated Timber Foundation Walls
Authors: Daneshvar Hossein , Fakhrzarei Mahboobeh , Imamura Fernanda , Chen Yuxiang , Deng Lijun , Chui Ying
Published: July 2022

There is widespread enthusiasm toward utilizing mass timber panels (MTP), mainly cross-laminated timber (CLT), in construction, including for the basements of low-rise buildings. CLT is deemed a sustainable alternative to the widely used concrete foundation walls due to significant advantages such as less vulnerability to cracking due to uneven load distribution and presence of concentrated loads, higher thermal resistance, less construction time due to whole-wall prefabrication and installation, and less detrimental environmental effects. This study is part of an extensive research program aimed at developing the structural analysis and design concepts and methodology for constructing house foundation walls using MTPs, focusing on the usage of CLT. After comparison of CLT basements with their equivalent concrete ones from the sustainability point of view, and a brief discussion on geotechnical and hygrothermal considerations, the main theme of the article includes the structural analysis and design methodology, requirements, and the procedure to achieve a reliable and efficient design of a CLT basement. A simplified analysis procedure to design the laminate thicknesses and the number of layers in CLT foundation walls for different scenarios considering various variables such as soil type and backfill height is discussed, and results in the form of pre-engineered design tables are provided. The findings of this study demonstrate that, depending on the soil type and backfill height, 3–7-ply CLT panels would be needed for net wall heights of up to 3 m. Additionally, advanced finite element analyses are performed on sample architypes to validate the simplified analysis procedure used for design. It is shown that the proposed analysis procedure and the pre-engineered tables produce conservative and efficient results.
Analytical Study Regarding the Seismic Response of a Moment-Resisting (MR) Reinforced Concrete (RC) Frame System with Reduced Cross Sections of the RC Beams
Authors: Sococol Ion , Mihai Petru , Petrescu Tudor-Cristian , Nedeff Florin , Nedeff Valentin , Agop Maricel
Published: July 2022

In the last few decades, a series of earthquakes were recorded which pointed out several deficiencies regarding the ductile seismic response of MR RC frame structures. Thus, the research problem centres around the failure mechanisms registered by the structures, which differ from the general notions of seismic response commonly found in current design standards and norms regarding seismic actions. In these conditions, in the present paper—by using comparative methods—the analytical validation of the solution of plastic hinge concentration and seismic energy dissipation in the marginal beam areas is proposed. Therefore, the RC beam sections were reduced (weakened) in the marginal areas which exhibit a plastic deformation potential, as well as in the corner areas of concrete slabs with vertical rectangular holes. The significant outcomes of this research imply the partial “guiding” of plastic hinges in the zones adjacent to beam ends. Furthermore, a reduction of both the negative effects of horizontal rigidization of the beams and the cracking and plastic deformation effects of beam-column frame joints was observed. With these technical implications, a complex mechanism of plastic deformation of MR RC frame models is registered in which all lateral elements (including RC columns) participate in the dissipation of seismic energy, without the occurrence of the “weak storey” mechanism for any of the analytical RC frame models. Furthermore, it is possible to observe the partial formation of the global plastic mechanism “Strong Columns—Weak Beams” (SCWB) for some of the structural models. Finally, the analytically studied innovative element regarding the improvement of the seismic response of pure MR RC frame structures is successfully validated.
Association between Health-Related Quality of Life and Completion of First-Line Treatment among Lung Cancer Patients
Authors: Levinsen Anne Katrine G. , Dalton Susanne O. , Andersen Ingelise , Mellemgaard Anders , Oksen Marianne S. , Saltbæk Lena , Hansen Nina H. G. , Carlsen Signe , Kjaer Trille K.
Published: July 2022

Experts recommend assessing lung cancer patients’ health-related quality of life (HRQOL) in the diagnostic evaluation. We investigated the association between HRQOL and completion of first-line treatment among lung cancer patients in a prospective cohort study. Clinical information on lung cancer patients was obtained from medical records, and information on quality of life and lung cancer-related symptoms was obtained through questionnaires at time of diagnosis. We used directed acyclic graphs to identify potential confounders and mediators between HRQOL and completion of first-line treatment. The association between functioning levels and symptoms and completion of first-line oncological treatment was estimated as odds ratios, with 95% confidence intervals, in logistic regression models. In all, 137 patients (52% men, mean age: 69 years) participated, out of 216 invited. Patients who reported reduced functioning had significantly increased ORs for not completing first-line treatment: poor physical function (OR 4.44), role function (OR 6.09), emotional function (OR 5.86), and social function (OR 3.13). Patients with fatigue (OR 7.55), pain (OR 6.07), appetite loss (OR 4.66), and financial difficulties (OR 17.23) had significantly increased ORs for not completing the first-line treatment. Reduced functioning and presence of symptoms were associated with not completing first-line treatment. An assessment of HRQOL could potentially aid the diagnostic evaluation and treatment planning for lung cancer patients.
Stem Cell Theory of Cancer: Implications for Translational Research from Bedside to Bench
Authors: Tu Shi-Ming , Singh Sunny R. , Arnaoutakis Konstantinos , Malapati Sindhu , Bhatti Sajjad A. , Joon Aron Y. , Atiq Omar T. , Pisters Louis L.
Published: July 2022

A stem cell theory of cancer considers genetic makeup in the proper cellular context. It is a unified theory of cancer that unites the genome with the epigenome, links the intracellular with the extracellular, and connects the cellular constituents and compartments with the microenvironment. Although it allies with genomic medicine, it is better aligned with integrated medicine. In this perspective, we focus on translational research in cancer care. We expose some intrinsic fallacies in translational research when it relates to the basic principles of the scientific method in the care of patients with genomic medicine versus integrated medicine. We postulate that genomic medicine may be at the root of many failed efforts in drug development and data reproducibility. We propose an alternate heuristic approach that may expedite the development of safe and effective treatments and minimize the generation of unproductive pharmaceutical products and nonreproducible experimental results. Importantly, a heuristic approach emphasizes the role of a pertinent scientific theory and distinguishes therapy development from drug development, such that we discover not only useful drugs but also better ways to use them in order to optimize patient care and maximize clinical outcomes.
Reduction of LPAR1 Expression in Neuroblastoma Promotes Tumor Cell Migration
Authors: Liu Xiangjun , Pei Mengmiao , Yu Yongbo , Wang Xiaolin , Gui Jingang
Published: July 2022

Neuroblastoma is the most common extracranial solid tumor in children. Tumor metastasis in high-risk NB patients is an essential problem that impairs the survival of patients. In this study, we aimed to use a comprehensive bioinformatics analysis to identify differentially expressed genes between NB and control cells, and to explore novel prognostic markers or treatment targets in tumors. In this way, FN1, PIK3R5, LPAR6 and LPAR1 were screened out via KEGG, GO and PPI network analysis, and we verified the expression and function of LPAR1 experimentally. Our research verified the decreased expression of LPAR1 in NB cells, and the tumor migration inhibitory effects of LPA on NB cells via LPAR1. Moreover, knockdown of LPAR1 promoted NB cell migration and abolished the migration-inhibitory effects mediated by LPA-LPAR1. The tumor-suppressing effects of the LPA-LPAR1 axis suggest that LPAR1 might be a potential target for future treatment of NB.
The Potential and Emerging Role of Quantitative Imaging Biomarkers for Cancer Characterization
Authors: Tharmaseelan Hishan , Hertel Alexander , Rennebaum Shereen , Nörenberg Dominik , Haselmann Verena , Schoenberg Stefan O. , Froelich Matthias F.
Published: July 2022

Similar to the transformation towards personalized oncology treatment, emerging techniques for evaluating oncologic imaging are fostering a transition from traditional response assessment towards more comprehensive cancer characterization via imaging. This development can be seen as key to the achievement of truly personalized and optimized cancer diagnosis and treatment. This review gives a methodological introduction for clinicians interested in the potential of quantitative imaging biomarkers, treating of radiomics models, texture visualization, convolutional neural networks and automated segmentation, in particular. Based on an introduction to these methods, clinical evidence for the corresponding imaging biomarkers—(i) dignity and etiology assessment; (ii) tumoral heterogeneity; (iii) aggressiveness and response; and (iv) targeting for biopsy and therapy—is summarized. Further requirements for the clinical implementation of these imaging biomarkers and the synergistic potential of personalized molecular cancer diagnostics and liquid profiling are discussed.
Extracellular Citrate Treatment Induces HIF1α Degradation and Inhibits the Growth of Low-Glycolytic Hepatocellular Carcinoma under Hypoxia
Authors: Kim Seon Yoo , Kim Dongwoo , Kim Jisu , Ko Hae Young , Kim Won Jin , Park Youngjoo , Lee Hye Won , Han Dai Hoon , Kim Kyung Sik , Park Sunghyouk , Lee Misu , Yun Mijin
Published: July 2022

HCC is well known for low glycolysis in the tumors, whereas hypoxia induces glycolytic phenotype and tumor progression. This study was conducted to evaluate the expression of SLCs in human HCCs and investigated whether extracellular nutrient administration related to SLCs in low-glycolytic HCC can prevent hypoxic tumor progression. SLCs expression was screened according to the level of glycolysis in HCCs. Then, whether extracellular nutrient treatment can affect hypoxic tumor progression, as well as the mechanisms, were evaluated in an in vitro cell line and an in vivo animal model. Low-glycolytic HCCs showed high SLC13A5/NaCT and SLC16A1/MCT1 but low SLC2A1/GLUT1 and HIF1α/HIF1α expression. Especially, high SLC13A5 expression was significantly associated with good overall survival in the Cancer Genome Atlas (TCGA) database. In HepG2 cells with the highest NaCT expression, extracellular citrate treatment upon hypoxia induced HIF1α degradation, which led to reduced glycolysis and cellular proliferation. Finally, in HepG2-animal models, the citrate-treated group showed smaller tumor with less hypoxic areas than the vehicle-treated group. In patients with HCC, SLC13A5/NaCT is an important SLC, which is associated with low glycolysis and good prognosis. Extracellular citrate treatment induced the failure of metabolic adaptation to hypoxia and tumor growth inhibition, which can be a potential therapeutic strategy in HCCs.
An Integrated Bioinformatics Analysis towards the Identification of Diagnostic, Prognostic, and Predictive Key Biomarkers for Urinary Bladder Cancer
Authors: Sarafidis Michail , Lambrou George I. , Zoumpourlis Vassilis , Koutsouris Dimitrios
Published: July 2022

Bladder cancer (BCa) is one of the most prevalent cancers worldwide and accounts for high morbidity and mortality. This study intended to elucidate potential key biomarkers related to the occurrence, development, and prognosis of BCa through an integrated bioinformatics analysis. In this context, a systematic meta-analysis, integrating 18 microarray gene expression datasets from the GEO repository into a merged meta-dataset, identified 815 robust differentially expressed genes (DEGs). The key hub genes resulted from DEG-based protein–protein interaction and weighted gene co-expression network analyses were screened for their differential expression in urine and blood plasma samples of BCa patients. Subsequently, they were tested for their prognostic value, and a three-gene signature model, including COL3A1, FOXM1, and PLK4, was built. In addition, they were tested for their predictive value regarding muscle-invasive BCa patients’ response to neoadjuvant chemotherapy. A six-gene signature model, including ANXA5, CD44, NCAM1, SPP1, CDCA8, and KIF14, was developed. In conclusion, this study identified nine key biomarker genes, namely ANXA5, CDT1, COL3A1, SPP1, VEGFA, CDCA8, HJURP, TOP2A, and COL6A1, which were differentially expressed in urine or blood of BCa patients, held a prognostic or predictive value, and were immunohistochemically validated. These biomarkers may be of significance as prognostic and therapeutic targets for BCa.
Exonucleases: Degrading DNA to Deal with Genome Damage, Cell Death, Inflammation and Cancer
Authors: Manils Joan , Marruecos Laura , Soler Concepció
Published: July 2022

Although DNA degradation might seem an unwanted event, it is essential in many cellular processes that are key to maintaining genomic stability and cell and organism homeostasis. The capacity to cut out nucleotides one at a time from the end of a DNA chain is present in enzymes called exonucleases. Exonuclease activity might come from enzymes with multiple other functions or specialized enzymes only dedicated to this function. Exonucleases are involved in central pathways of cell biology such as DNA replication, repair, and death, as well as tuning the immune response. Of note, malfunctioning of these enzymes is associated with immune disorders and cancer. In this review, we will dissect the impact of DNA degradation on the DNA damage response and its links with inflammation and cancer.
A Novel Cell Delivery System Exploiting Synergy between Fresh Titanium and Fibronectin
Authors: Hirota Makoto , Hori Norio , Sugita Yoshihiko , Ikeda Takayuki , Park Wonhee , Saruta Juri , Ogawa Takahiro
Published: July 2022

Delivering and retaining cells in areas of interest is an ongoing challenge in tissue engineering. Here we introduce a novel approach to fabricate osteoblast-loaded titanium suitable for cell delivery for bone integration, regeneration, and engineering. We hypothesized that titanium age influences the efficiency of protein adsorption and cell loading onto titanium surfaces. Fresh (newly machined) and 1-month-old (aged) commercial grade 4 titanium disks were prepared. Fresh titanium surfaces were hydrophilic, whereas aged surfaces were hydrophobic. Twice the amount of type 1 collagen and fibronectin adsorbed to fresh titanium surfaces than aged titanium surfaces after a short incubation period of three hours, and 2.5-times more fibronectin than collagen adsorbed regardless of titanium age. Rat bone marrow-derived osteoblasts were incubated on protein-adsorbed titanium surfaces for three hours, and osteoblast loading was most efficient on fresh titanium adsorbed with fibronectin. The number of osteoblasts loaded using this synergy between fresh titanium and fibronectin was nine times greater than that on aged titanium with no protein adsorption. The loaded cells were confirmed to be firmly attached and functional. The number of loaded cells was strongly correlated with the amount of protein adsorbed regardless of the protein type, with fibronectin simply more efficiently adsorbed on titanium surfaces than collagen. The role of surface hydrophilicity of fresh titanium surfaces in increasing protein adsorption or cell loading was unclear. The hydrophilicity of protein-adsorbed titanium increased with the amount of protein but was not the primary determinant of cell loading. In conclusion, the osteoblast loading efficiency was dependent on the age of the titanium and the amount of protein adsorption. In addition, the efficiency of protein adsorption was specific to the protein, with fibronectin being much more efficient than collagen. This is a novel strategy to effectively deliver osteoblasts ex vivo and in vivo using titanium as a vehicle.
Mechanisms for Bile Acids CDCA- and DCA-Stimulated Hepatic Spexin Expression
Authors: Lai Qi , Ma Yanhua , Bai Jin , Zhuang Min , Pei Shaofei , He Ni , Yin Junlin , Fan Baomin , Bian Zhaoxiang , Zeng Guangzhi , Lin Chengyuan
Published: July 2022

Spexin (SPX) is a novel peptide involved in glucose and lipid metabolism and suppresses hepatic total bile acid levels by inhibiting hepatic cholesterol 7α-hydroxylase 1 expression. As important mediators for glycolysis/gluconeogenesis and lipid metabolism, the effects of bile acids on SPX expression is yet to be understood. By using SMMC7721 and BEL-7402 cell lines, we screened the effects of bile acids and found that chenodeoxycholic acid (CDCA) and deoxycholic acid (DCA) can stimulate SPX gene transcription. Both CDCA and DCA were able to stimulate SPX mRNA expression in the liver but not colon and ileum in mice. In SMMC7721 and BEL-7402 cells, CDCA- and DCA-induced SPX promoter activity was mimicked by bile acid receptor FXR and TGR5 activation and suppressed by FXR and TGR5 silencing. Adenylate cyclase (AC)/cyclic adenosine monophosphate (cAMP) activators significantly increased SPX promoter activity whereas the inhibitors for AC/CAMP/protein kinase A (PKA) and mitogen-activated protein kinases (MAPK) pathway attenuated CDCA- and DCA-induced SPX transcription. Thus, CDCA and DCA stimulate SPX expression at the hepatic level through FXR and TGR5 mediated AC/cAMP/PKA and MAPK cascades.
Surgical Treatment of Short Bowel Syndrome—The Past, the Present and the Future, a Descriptive Review of the Literature
Authors: Muff Julian L. , Sokolovski Filipp , Walsh-Korb Zarah , Choudhury Rashikh A. , Dunn James C. Y. , Holland-Cunz Stefan G. , Vuille-dit-Bille Raphael N.
Published: July 2022

Short bowel syndrome (SBS) is a devastating disorder with both short- and long-term implications for patients. Unfortunately, the prevalence of SBS has doubled over the past 40 years. Broadly speaking, the etiology of SBS can be categorized as congenital or secondary, the latter typically due to extensive small bowel resection following diseases of the small intestine, e.g., necrotizing enterocolitis, Hirschsprung’s disease or intestinal atresia. As of yet, no cure exists, thus, conservative treatment, primarily parenteral nutrition (PN), is the first-line therapy. In some cases, weaning from PN is not possible and operative therapy is required. The invention of the longitudinal intestinal lengthening and tailoring (LILT or Bianchi) procedure in 1980 was a major step forward in patient care and spawned further techniques that continue to improve lives for patients with severe SBS (e.g., double barrel enteroplasty, serial transverse enteroplasty, etc.). With this review, we aim to provide an overview of the clinical implications of SBS, common conservative therapies and the development of operative techniques over the past six decades. We also provide a short outlook on the future of operative techniques, specifically with respect to regenerative medicine.
LGBTQ+ Youth Health: An Unmet Need in Pediatrics
Authors: Diana Pierluigi , Esposito Susanna
Published: July 2022

At present, lesbian, gay, bisexual, transgender, queer and intersex people (LGBTQ+) are increasingly being empowered to freely express themselves, particularly young people and rising generations. Although data underline the trend of more open expression of different sexual orientations and gender identities, LGBTQ+ adolescents still suffer discrimination in the health care framework. Inclusive care by providers to look after the health of LGBTQ+ indiviuals is needed. Pediatricians are often the first health care providers for LGBTQ+ youth facing their sexual and gender identities. Unfortunately, pediatricians have limited knowledge about LGBTQ+ issues, which keeps them from fulfilling the specific needs of LGBTQ+ youth. The purpose of this review is to frame the most important aspects of LGBTQ+ youths’ lives, including risks, difficulties and needs, that pediatricians should investigate and meet to provide these youth with better and more individualizedassistance regarding their health. A literature analysis showed that pediatricians have insufficient knowledge of and comfort with several items regarding the management of LGBTQ+ youths. Increased awareness and knowledge of the specific and exclusive needs of LGBTQ+ adolescents are mandatory, including dedicated pediatric LGBTQ+ health care training. This would give them the opportunity to forward an inclusive health care system, thus reducing the risks related to stigma, bullying and family rejection and promoting sex education. Further studies are needed to better evaluate the prevalence of LGBTQ+ youths, gender-based medicine in pediatrics and the effects of COVID-19 on the LGBTQ+ younth population due to increased risks of psychosocial suffering, isolation and mental diseases.
Probabilistic Assessment of Biodeterioration Effects on Reinforced Concrete Sewers
Authors: Marquez-Peñaranda Jorge Fernando , Sanchez-Silva Mauricio , Bastidas-Arteaga Emilio
Published: July 2022

The worldwide current practice of the structural design of sewers is based on procedures which usually include the effects caused by chemical and biological deterioration. However, in the last few decades, many sewer pipes have been designed using reinforced concrete which have succinctly considered such deterioration promoters. Indeed, knowledge related to reinforced concrete deterioration processes has become an important issue when forecasting the expected or remaining lifespan of sewers. Within these processes, thickness and strength losses and porosity augments have been found to be the result of the vital activity of sulfur-oxidizing bacteria and some types of fungus. This paper presents a rational methodology that uses biodeterioration measurements to describe how biodeterioration effects can affect the probability of failure during the lifetime of sewers. The probability of failure was obtained using Monte Carlo simulations based on numerical sampling from lognormal and uniform distributions. The concrete and reinforcement strength, geometric properties, H2S concentration in the headspace, and load values were considered as the main sources of uncertainty. The results indicate that the expected service lifespan can vary between 55 and 37 years for low and high H2S concentrations, respectively.
Photonics of Halogenated Zinc(II) and Cadmium(II) Dipyrromethene Complexes
Authors: Aksenova Iuliia , Bocharnikova Elena , Ashmarina Maria
Published: July 2022

This article compares spectroscopic properties of the series of dipyrromethene dyes, namely their complexes of boron (III), zinc(II) and cadmium(II) with the halogenated ligands of the same structure. Absorption and emission spectra, lifetimes of long-lived emission and quantum yields of luminescence were studied as the functions of molecular structure of dipyrromethene complexes. The role of the position and nature of a substituent in a ligand, polarity of a solvent and temperature of media were also investigated. The studies demonstrate that replacing the central atom boron(III) by zinc(II) decreases the fluorescence quantum yield, indicating the increased role of non-radiative processes in excitation energy deactivations such as intersystem crossings. In addition, according to the heavy atom effect, the efficiency of intersystem crossings in halogen-substituted zinc(II) and cadmium(II) dipyrromethene complexes is higher than in the corresponding boron fluoride dipyrromethenes (BODIPY), which leads to increase in phosphorescence at low temperatures (frozen solutions). The obtained results make it possible to carry out further investigations of potential sensory properties that are required for systematic use of halogenated dipyrromethene complexes for the creation of modern optical oxygen sensors and singlet oxygen photosensitizers for photodynamic therapy or photocatalytic oxidative reactions.
High-Temperature Electronic Transport Properties of PEDOT:PSS Top-Contact Molecular Junctions with Oligophenylene Dithiols
Authors: Seo Dong-Hyoup , Im Kyungjin , Song Hyunwook
Published: July 2022

In this study, we investigated the high-temperature electronic transport behavior of spin-coated PEDOT:PSS top-contact molecular ensemble junctions based on self-assembled monolayers (SAMs) of oligophenylene dithiols. We observed irreversible temperature-dependent charge transport at the high-temperature regime over 320 K. The effective contact resistance and normalized resistance decreased with increasing temperature (320 to 400 K), whereas the tunneling attenuation factor was nearly constant irrespective of temperature change. These findings demonstrate that the high-temperature transport properties are not dominated by the integrity of SAMs in molecular junctions, but rather the PEDOT:PSS/SAMs contact. Transition voltage spectroscopy measurements indicated that the contact barrier height of the PEDOT:PSS/SAMs is lowered at elevated temperatures, which gives rise to a decrease in the contact resistance and normalized resistance. The high-temperature charge transport through these junctions is also related to an increase in the grain area of PEDOT cores after thermal treatment. Moreover, it was found that there was no significant change in either the current density or normalized resistance of the annealed junctions after 60 days of storage in ambient conditions.
Diagnostic Accuracy of PET/CT or PET/MRI Using PSMA-Targeting Radiopharmaceuticals in High-Grade Gliomas: A Systematic Review and a Bivariate Meta-Analysis
Authors: Muoio Barbara , Albano Domenico , Dondi Francesco , Bertagna Francesco , Garibotto Valentina , Kunikowska Jolanta , Piccardo Arnoldo , Annunziata Salvatore , Espeli Vittoria , Migliorini Denis , Treglia Giorgio
Published: July 2022

Background: Several studies proposed the use of positron emission tomography (PET) with Prostate Specific Membrane Antigen (PSMA)-targeting radiopharmaceuticals in brain tumors. Our aim is to calculate the diagnostic accuracy of these methods in high-grade gliomas (HGG) with a bivariate meta-analysis. Methods: A comprehensive literature search of studies on the diagnostic accuracy of PET/CT or PET/MRI with PSMA-targeting radiopharmaceuticals in HGG was performed. Original articles evaluating these imaging methods both in the differential diagnosis between HGG and low-grade gliomas (LGG) and in the assessment of suspicious HGG recurrence were included. Pooled sensitivity, specificity, positive and negative likelihood ratios (LR+ and LR-), and diagnostic odds ratio (DOR) including 95% confidence intervals (95% CI) were calculated. Statistical heterogeneity was also assessed using the I2 test. Results: The meta-analysis of six selected studies (157 patients) provided the following results about PET/CT or PET/MRI with PSMA-targeting radiopharmaceuticals in the diagnosis of HGG: sensitivity 98.2% (95% CI: 75.3–99.9%), specificity 91.2% (95% CI: 68.4–98.1%), LR+ 4.5 (95% CI: 2.2–9.3), LR− 0.07 (95% CI: 0.04–0.15), and DOR 70.1 (95% CI: 19.6–250.9). No significant statistical heterogeneity among the included studies was found (I2 = 0%). Conclusions: the quantitative data provided demonstrate the high diagnostic accuracy of PET/CT or PET/MRI with PSMA-targeting radiopharmaceuticals for HGG detection. However, more studies are needed to confirm the promising role of PSMA-targeted PET in this clinical setting.
Franseen Needles May Be Promising for Improving the Sampling Adequacy of EUS-FNA for Subepithelial Lesions
Authors: Kasuga Noriki , Kurita Yusuke , Tanida Emiko , Yagi Shin , Suzuki Ko , Hasegawa Sho , Sato Takamitsu , Hosono Kunihiro , Kato Shingo , Sekino Yusuke , Kobayashi Noritoshi , Endo Itaru , Kubota Kensuke , Nakajima Atsushi
Published: July 2022

Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is useful in diagnosing subepithelial lesions (SELs), and adequate tissue sampling is necessary to differentiate between benign and malignant diseases to determine therapeutic strategies. This study aimed to evaluate sampling adequacy and diagnostic performance of EUS-FNA for SELs with Franseen needles. This retrospective study enrolled 130 patients who underwent EUS-FNA with a 22-gauge needle for SELs from January 2010 to March 2021. We compared sampling adequacy and predictive factors influencing the sampling adequacy of EUS-FNA for SELs between Franseen and conventional needles. The sampling adequacy rates were 95.0% (38/40) with Franseen needles and 76.7% (69/90) with conventional needles (p = 0.011). The mean number of punctures with Franseen needles (2.80) was significantly less than that with conventional needles (3.42) (p < 0.001). In the multivariate analysis, the use of Franseen needles (p = 0.029; odds ratio [OR], 5.37; 95% confidence interval [CI], 1.18–23.36) was an independent factor influencing the sampling adequacy. Compared to conventional needles, the Franseen needle could play a vital role in accurately diagnosing SELs by yielding better sampling adequacy and reducing the number of passes.
Optimization of Patient Management in the Gynecology Emergency Department Using Point-of-Care Beta hCG
Authors: Brousse Mehdi , Bargnoux Anne-Sophie , Courtais-Coulon Caroline , Badiou Stéphanie , Kuster Nils , Compan Clara , Fuchs Florent , Cristol Jean-Paul
Published: July 2022

Background: Point-of-care testing (POCT) provides shorter turn-around times and, in many cases, potentially improves medical decision making. The AQT90 FLEX® benchtop immunoanalyzer (Radiometer Medical ApS, Copenhagen, Denmark) allows for the determination of beta-human chorionic gonadotropin (βhCG) in 18 min. The main aim of this study was to evaluate the impact of measuring βhCG using the AQT90 analyzer in the gynecology emergency department (ED) compared to the standard practice of using central laboratory blood testing on the patient length of stay (LOS). Methods: The evaluation consisted of two parts. The first one, conducted in the central laboratory, focused on the analytical performances of the AQT βhCG assay. The second one, conducted in the ED, aimed at determining the impact of POCT βhCG implementation on the timeframe in which ED patients require βhCG assessment. Results: The within-lab imprecisions at the mean values of 17 and 287 IU/L were 2.7% and 3.7%, respectively. Using Deming regression (n = 60), the following equation was obtained in the central lab: AQT90 βhCG = 1.1 Roche βhCG—12.9 (r = 0.997). The implementation of POCT βhCG in the ED significantly reduced patient LOS (145 (90–212) min vs. 205 (155–265) with and without AQT90, respectively, p < 0.001). At the 2 IU/L decision level, a 99.7% agreement with the Roche assay was reported (kappa statistics, 0.99). Conclusions: We confirm that the analytical qualities of the AQT 90 were in line with those obtained in the central lab. The implementation of the POCT βhCG is associated with a shorter LOS in the ED due to the faster availability of the results and the faster decision-making possibilities.
Detecting and Monitoring Periprosthetic Joint Infection by Using Electrical Bioimpedance Spectroscopy: A Preliminary Case Study
Authors: Balato Marco , Petrarca Carlo , Arpaia Pasquale , Moccaldi Nicola , Mancino Francesca , Carleo Giusy , Minucci Simone , Mariconda Massimo , Balato Giovanni
Published: July 2022

A method to detect the presence of infection after Total Joint Arthroplasty is presented. The method is based on Electrical Bioimpedance Spectroscopy and guarantees low latency, non-invasiveness, and cheapness with respect to the state of art. Experimental measurements were carried out on a singular patient who had already undergone bilateral Total Knee Arthroplasty. He was affected by a hematogenous Periprosthetic Joint Infections on the left knee. The right knee was adopted as the reference. Measurements were acquired once before the surgical procedure (Diagnosis Phase) and twice in the postoperative phases (Monitoring Phase). The most relevant frequency range, for diagnosis and monitoring phases, was found to be between 10 kHz to 50 kHz. The healing trend predicted by the decrease of impedance magnitude spectrum was reflected in clinical and laboratory results. In addition, one month after the last acquisition (two months after the surgery), the patient fully recovered, confirming the prediction of the Electrical Bioimpedance Spectroscopy technique.
Culturo-Scientific Storytelling
Authors: Goorney Simon , Foti Caterina , Santi Lorenzo , Sherson Jacob , Yago Malo Jorge , Chiofalo Maria Luisa
Published: July 2022

In this article, we reflect on the functions of outreach in developing the modern scientific mind, and discuss its essential importance in the modern society of rapid technological development. We embed our approach to outreach in culturo-scientific thinking. This is constituted by embracing disciplinary thinking (in particular creativity) whilst appreciating the epistemology of science as an evolving dialogue of ideas, with numerous alternative perspectives and uncertain futures to be managed. Structuring scientific knowledge as an assemblage of interacting and evolving discipline-cultures, we conceive of a culturo-scientific storytelling to bring about positive transformations for the public in these thinking skills and ground our approach in quantum science and technologies (QST). This field has the potential to generate significant changes for the life of every citizen, and so a skills-oriented approach to its education, both formal and non-formal, is essential. Finally, we present examples of such storytelling in the case of QST, the classification and evaluation of which correspond to future work in which this narrative approach is studied in action.
IRSDet: Infrared Small-Object Detection Network Based on Sparse-Skip Connection and Guide Maps
Authors: Xi Xiaoli , Wang Jinxin , Li Fang , Li Dongmei
Published: July 2022

Detecting small objects in infrared images remains a challenge because most of them lack shape and texture. In this study, we proposed an infrared small-object detection method to improve the capacity for detecting thermal objects in complex scenarios. First, a sparse-skip connection block is proposed to enhance the response of small infrared objects and suppress the background response. This block is used to construct the detection model backbone. Second, a region attention module is designed to emphasize the features of infrared small objects and suppress background regions. Finally, a batch-averaged biased classification loss function is designed to improve the accuracy of the detection model. The experimental results show that the proposed small-object detection framework significantly increases precision, recall, and F1-score, showing that, compared with the current advanced detection models for small-object detection, the proposed detection framework has better performance in infrared small-object detection under complex backgrounds. The insights gained from this study may provide new ideas for infrared small object detection and tracking.
Modeling of Satellite Constellation in Modelica and a PHM System Framework Driven by Model Data Hybrid
Authors: Liu Chan , Chen Liping , Ding Jianwan , Shangguan Duansen
Published: July 2022

The new generation of low-earth-orbit (LEO) satellite constellation systems has the characteristics of low delay, strong signal and global coverage, and it is an important direction for the development of next-generation communication technology. A major disadvantage is that the constellation system is huge, often composed of hundreds or thousands of satellites, which puts forward high requirements for the design and health management of the constellation system, and the existing telemetry data monitoring system cannot meet the actual needs. CPS is a multidimensional complex system that integrates computation, communication and control (3C). Through the deep integration and cooperation of 3C, the real-time monitoring and dynamic control of large-scale engineering systems are realized, which is completely suitable for the operation and maintenance requirements of the satellite constellation system. This paper firstly establishes the entire satellite constellation system model, which is integrated from the satellite multidomain system, the constellation orbit environment system and the communication link system. Then, according to the technical concept of cyber-physical systems (CPS), an implementation framework of a prognostics and health (PHM) system driven by a model–data hybrid for satellite constellation systems is proposed. The framework is based on model simulation data and telemetry data and combines virtual and real data fusion, fault diagnosis, simulation prediction and other technologies to generate enhanced data to drive the effective operation of the PHM system. Finally, a verification case is designed to prove that the satellite constellation health management system implemented under this framework has a positive effect on the reliable operation and maintenance of the satellite constellation system.
Telemedicine System Applicability Using Drones in Pandemic Emergency Medical Situations
Authors: Nedelea Paul Lucian , Popa Tudor Ovidiu , Manolescu Emilian , Bouros Catalin , Grigorasi Gabriela , Andritoi Doru , Pascale Catalin , Andrei Avramescu , Cimpoesu Diana Carmen
Published: July 2022

Drones have evolved significantly in recent years, acquiring greater autonomy and carrier capacity. Therefore, drones can play a substantial role in civil medicine, especially in emergency situations or for the detection and monitoring of disease spread, such as during the COVID-19 pandemic. The aim of this paper is to present the real possibilities of using drones in field rescue operations, as well as in nonsegregated airspace, in order to obtain solutions for monitoring activities and aerial work in support of the public health system in crisis situations. The particularity of our conceptual system is the use of a “swarm” of fast drones for aerial reconnaissance that operate in conjunction, thus optimizing both the search and identification time while also increasing the information area and the operability of the system. We also included a drone with an RF relay, which was connected to a hub drone. If needed, a carrier drone with medical supplies or portable devices can be integrated, which can also offer two-way audio and video communication capabilities. All of these are controlled from a mobile command center, in real time, connected also to the national dispatch center to shorten the travel time to the patient, provide support with basic but life-saving equipment, and offer the opportunity to access remote or difficult-to-reach places. In conclusion, the use of drones for medical purposes brings many advantages, such as quick help, shortened travel time to the patient, support with basic but life-saving equipment, and the opportunity to access remote or difficult-to-reach places.
Friction Coefficients of Chestnut (Castanea sativa Mill.) Sawn Timber for Numerical Simulation of Timber Joints
Authors: Villar-García José Ramón , Vidal-López Pablo , Rodríguez-Robles Desirée , Moya Ignacio Manuel
Published: July 2022

This study focuses on the friction characteristics of chestnut sawn timber (Castanea sativa Mill.) of Spanish origin. The values of both the static and dynamic friction coefficients were determined, as this knowledge is of interest for the numerical simulation of the stress transmission in joints of timber structures. Therefore, two sets of tests were carried out combining different orthotropic orientations. Firstly, timber-to-timber tests were assessed to obtain the coefficients applicable to carpentry joints; secondly, timber-to-steel friction was also evaluated to determine the coefficients needed for mechanical joints with metal plates and dowels. The results presented a conventional behavior of friction, i.e., a maximum static value before sliding and a subsequent decrease. For timber-to-timber tests, global mean values of μs = 0.47 and μk = 0.36 were found, and the results were slightly higher than those obtained between pieces with the same orthotropic orientation and sliding direction. Regarding timber-to-steel tests, the resulting friction coefficients, as well as the difference existing between the static and dynamic values were lower (μs = 0.19 and μk = 0.17) compared to the timber-to-timber sets. The use of these results in numerical studies would allow for closer simulations in which chestnut wood is involved in friction. In addition, the values provided herein could be included as new data in standards that already consider other wood species.
Understory Plant Abundance Is More Important than Species Richness in Explaining Soil Nutrient Variation Following Afforestation on the Eastern Loess Plateau, China
Authors: Zhao Yao , Li Meng , Wang Baitian
Published: July 2022

Afforestation plays an important role in controlling soil erosion and nutrient loss on the Loess Plateau in China. However, previous studies on soil nutrient changes have mainly focused on the effects of tree species, whereas little is known about how changes in shrubs and herbs caused by afforestation drive soil nutrient changes. In this study, we examined the variation characteristics of understory vegetation and soil nutrients for different vegetation types. The results showed that compared to abandoned farmland, plantations significantly increased soil organic carbon and total nitrogen but had no significant effect on total phosphorus. Robinia pseudoacacia L. forests were more effective than Pinus tabuliformis Carr. forests in increasing soil nutrient content. In addition, herbaceous vegetation in the R. pseudoacacia forest better explained the soil nutrient variation, and herb abundance was the best explanatory variable; however, shrub vegetation contributed more to soil nutrient variation in the P. tabuliformis forest, and shrub abundance contributed the most. Accordingly, we determined that understory plant abundance, rather than species richness, may be the most important factor driving soil nutrient changes. Specifically, herb abundance in the R. pseudoacacia forest may drive soil nutrient changes mainly by regulating herb biomass and litter biomass. By contrast, shrub abundance in the P. tabuliformis forest indirectly affected soil organic carbon mainly by altering shrub biomass. Furthermore, although the phylogenetic relationships had less effect on soil nutrients than species composition, they also made important contributions. Therefore, the phylogenetic relationships should also be considered in addition to species composition when assessing the impact of vegetation on soil properties in the future.
Production of Gamma-Aminobutyric Acid by Levilactobacillus brevis CD0817 by Coupling Fermentation with Self-Buffered Whole-Cell Catalysis
Authors: Li Haixing , Sun Tianyi , Jia Mengya , Wang Lingqin , Wei Cheng , Pei Jinfeng , Lin Zhiyu , Wang Shuixing
Published: July 2022

There is a recent trend of using lactic acid bacteria for the production of gamma-aminobutyric acid (GABA). This study described a method that combines fermentation and self-buffered whole-cell catalysis for the efficient production of GABA using Levilactobacillus brevis CD0817. Upon the completion of GABA fermentation, cells were recovered to conduct whole-cell catalysis by which the substrate L-glutamic acid was catalytically decarboxylated to GABA. L-glutamic acid itself maintained the acidity essential for decarboxylation. To maximize the whole-cell catalysis ability, the effects of the cell culture method, catalysis temperature, catalysis time, cell concentration, and L-glutamic acid dosage were investigated. The results illustrate that the cells that were cultivated for 16 h in a fermentation medium supplemented with 20.0 g/L of glucose were the most suitable for the whole-cell catalytic production of GABA. At 16 h, the fermentative GABA content reached 204.2 g/L. Under optimized whole-cell catalytic conditions (temperature 45.0 °C, time 12.0 h, wet cells 25.0 g/L, and L-glutamic acid 120.0 g/L), 85.1 g/L of GABA was obtained, with 3.7 ± 0.9 g/L of substrate residue. GABA was recovered from the system by sequentially performing rotary vacuum evaporation, precipitation with ethanol, filtration with filter paper, and drying. The purity of the GABA product reached 97.1%, with a recovery rate of 87.0%. These data suggest that the proposed method has potential applications in the production of GABA.