bluuebunny/crossref_metadata_2025 huggingface dataset:
Changes in characteristics of inpatient respiratory conditions from 2019 to 2021 (before and during the COVID-19 pandemic)
Authors: Mojtahedi Zahra , Yoo Ji , Kim Pearl , Kim Yonsu , Shen Jay J. , Wang Bing-Long
Published: November 2023

Background

The COVID-19 pandemic has resulted in an increase in the number of individuals with respiratory conditions that require hospitalization, posing new challenges for the healthcare system. Recent respiratory condition studies have been focused on the COVID-19 period, with no comparison of respiratory conditions before and during the pandemic. This study aimed to examine hospital-setting respiratory conditions regarding potential changes in length of stay (LOS), mortality, and total charge, as well as socioeconomic disparities before and during the pandemic.

Methods

The study employed a pooled cross-sectional design based on the State Inpatient Data Nevada for 2019 (prior to the COVID-19 pandemic) and 2020–2021 (during the pandemic) and investigated all respiratory conditions, identified by the International Classification of Disease, 10th Revision codes (n = 227,338). Descriptive analyses were carried out for the three years. Generalized linear regression models were used for multivariable analyses. Outcome measures were hospital LOS, mortality, and total charges.

Results

A total of 227,338 hospitalizations with a respiratory condition were included. Hospitalizations with a respiratory condition increased from 65,896 in 2019 to 80,423 in 2020 and 81,018 in 2021. The average LOS also increased from 7.9 days in 2019 to 8.8 days in 2020 but decreased to 8.1 days in 2021; hospital mortality among patients with respiratory conditions increased from 7.7% in 2019 to 10.2% but decreased to 9.6% in 2021; and the total charges per discharge were $159,119, $162,151, and $161,733 from 2019 to 2021, respectively (after adjustment for the inflation rate). Hispanic, Asian, and other race patients with respiratory conditions were 1–3 times more likely than white patients to have higher mortality and LOS. Medicaid patients and non-White patients were predictors of a higher respiratory-related hospital total charge.

Conclusion

Demographic and socioeconomic factors were significantly associated with respiratory-related hospital utilization in terms of LOS, mortality, and total charge.
The accuracy of intraocular lens power calculation formulas based on artificial intelligence in highly myopic eyes: a systematic review and network meta-analysis
Authors: Zhou Yi , Dai Minhui , Sun Lingyu , Tang Xiangyi , Zhou Ling , Tang Zhiyao , Jiang Jian , Xia Xiaobo
Published: November 2023

Objective

To systematically compare and rank the accuracy of AI-based intraocular lens (IOL) power calculation formulas and traditional IOL formulas in highly myopic eyes.

Methods

We screened PubMed, Web of Science, Embase, and Cochrane Library databases for studies published from inception to April 2023. The following outcome data were collected: mean absolute error (MAE), percentage of eyes with a refractive prediction error (PE) within ±0.25, ±0.50, and ±1.00 diopters (D), and median absolute error (MedAE). The network meta-analysis was conducted by R 4.3.0 and STATA 17.0.

Results

Twelve studies involving 2,430 adult myopic eyes (with axial lengths >26.0 mm) that underwent uncomplicated cataract surgery with mono-focal IOL implantation were included. The network meta-analysis of 21 formulas showed that the top three AI-based formulas, as per the surface under the cumulative ranking curve (SUCRA) values, were XGBoost, Hill-RBF, and Kane. The three formulas had the lowest MedAE and were more accurate than traditional vergence formulas, such as SRK/T, Holladay 1, Holladay 2, Haigis, and Hoffer Q regarding MAE, percentage of eyes with PE within ±0.25, ±0.50, and ±1.00 D.

Conclusions

The top AI-based formulas for calculating IOL power in highly myopic eyes were XGBoost, Hill-RBF, and Kane. They were significantly more accurate than traditional vergence formulas and ranked better than formulas with Wang–Koch AL modifications or newer generations of formulas such as Barrett and Olsen.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42022335969.
Incidence of SARS-CoV-2 infection in children shortly after ending zero-COVID-19 policy in China on December 7, 2022: a cross-sectional, multicenter, seroepidemiological study
Authors: Zhou Yi-Hua , Xu Chenyu , Tao Yue , Gu Meng , Zhou Guiping , Zhou Wei , Jin Yue , Xie Jun , Xu Biyun , Zhou Wensan , Chen Junhao , Shi Weifeng
Published: November 2023

Background

China discontinued the zero-COVID-19 policy on December 7, 2022, and then COVID-19 surged mid-December 2022 through mid-January 2023. However, the actual incidence was unknown. This study aimed to estimate the incidence of SARS-CoV-2 infection in children shortly after ending the zero-COVID-19 policy.

Methods

This multicenter cross-sectional study included 1,065 children aged 8 months to 12 years from seven hospitals at six regions across Jiangsu province, based on the convenience sampling, from February 10 to March 10, 2023. Group I comprised 324 children aged 8 months–2 years without COVID-19 vaccination, group II consisted of 338 preschool children aged 3–5 years with varied vaccination history, and group III contained 403 primary school children aged 6–12 years with mostly vaccinated. The COVID-19 vaccines were composed of inactivated SARS-CoV-2. In addition, 96 children’s sera collected in 2014 were included as negative controls. IgG and IgM antibodies against nucleocapsid (N) and subunit 1 of spike (S1) of SARS-CoV-2 (anti-N/S1) were measured with commercial kits (YHLO Biotech, Shenzhen, China).

Results

None of the 96 children (5.1 ± 3.5 years; 58.3% boys) in 2014 was positive for anti-N/S1 IgG or IgM. Of the 1,065 children (5.0 ± 3.5 years; 56.0% boys), 988 (92.8%) were anti-N/S1 IgG positive but none was anti-N/S1 IgM positive. The positive rate of anti-N/S1 IgG in Group I, II, and III was 90.4, 88.5, and 98.3%, respectively, with significantly higher in group III than in groups I and II (p < 0.0001). The median antibody titers in group III (381.61 AU/ml) were much higher than that in group I (38.34 AU/ml) and II (51.88 AU/ml; p < 0.0001).

Conclusion

More than 90% children experienced SARS-CoV-2 infection shortly after ending zero-COVID-19 policy in China, much higher than estimated infections by other studies. The widespread SARS-CoV-2 infection in unvaccinated children should be influential on the policy of COVID-19 vaccination in children in the future.
Heterogeneous influence of neighborhood features on community satisfaction: a comparative study in Beijing’s urban and suburban communities
Authors: Jiang Huaxiong , Cai Runting , Yang Ding , Huang Zhuo , Song Jinping
Published: November 2023

Introduction

Community satisfaction contributes to urban planning, community development, and policy formulation. Yet, we lack comprehensive knowledge about how different neighborhood features impact satisfaction, especially across diverse community types.

Methods

Relied on a sample of 4,009 respondents in Beijing, this study examines the influence of neighborhood features on community satisfaction through neighborly interactions, focusing on the heterogeneity between urban and suburban communities, using structural equation models.

Results

The results reveal that community service and community management exert significant influences on community satisfaction, primarily mediated by the role of neighborly interactions. Then, transportation convenience positively influences community satisfaction in urban areas, while no housing property has a negative effect in suburban communities.

Discussion

These results highlight varied neighborhood effects on community satisfaction, informing tailored urban planning and policies that address unique traits and requirements of different communities.
Recovery of activities of daily living in COVID-19 patients requiring intensive care unit or medical care unit: an observational study on the role of rehabilitation in the subacute phase
Authors: Notarstefano Chiara , Bertolucci Federica , Miccoli Mario , Posteraro Federico
Published: November 2023

Purpose

This study aims to describe the functional status of a cohort of subacute COVID-19 patients treated in a dedicated rehabilitation unit and to compare functional outcomes between patients previously hospitalized in the intensive care unit (ICU group) and patients assisted in the medical care unit (MCU group).

Materials and methods

Clinical and functional evaluations were performed at admission and discharge. The functional status was assessed using Barthel index (BI), functional ambulation categories (FAC), trunk control test (TCT), and dysphagia outcome and severity score (DOSS). All patients received multidisciplinary tailored rehabilitation.

Results

We evaluated 171 patients (with a mean age of 67.7 ± 11.9 years, 117 were males), 110 coming from the ICU (with a mean age of 63.24 ± 10.9 years), and 61 coming from the MCU (with a mean age of 75.75 ± 9.09 years). The ICU group showed a worse functional status at admission compared with the MCU group [BI 2.5 (0–20) vs. 20 (10–60), FAC 0 (0–0) vs. 0 (0–2), TCT 61 (42–100) vs. 100 (61–100), DOSS 5 (1–7) vs. 7 (7–7)] and had significantly longer hospital stay. At discharge, all functional scales were improved with no statistically significant differences between the two groups.

Conclusion

Early rehabilitation of COVID-19 survivors improves functional recovery closing the initial gap between the ICU and MCU groups. In addition, it is effective to improve the functional outcome reducing the costs for longer-term assistance of COVID-19 patients.
Farnesyltransferase-inhibitors exert in vitro immunosuppressive capacity by inhibiting human B-cells
Authors: Xu Shilei , Dolff Sebastian , Mülling Nils , Bachmann Hagen S. , Dai Yang , Lindemann Monika , Sun Ming , Witzke Oliver , Kribben Andreas , Wilde Benjamin
Published: November 2023

Objectives

Farnesyltransferase inhibitors (FTI), which inhibit the prenylation of Ras GTPases, were developed as anti-cancer drugs. As additional target proteins for prenylation were identified in the past, it is likely that FTI have potential value for therapeutic purposes beyond cancer. The effect of FTI on B-cells remains unclear. To address this issue, we investigated the effects of in vitro FTI treatment on effector and regulatory B-cells in healthy controls and renal transplant patients.

Methods

For this purpose, B-cells were isolated from the peripheral blood of healthy controls and renal transplant patients. Purified B-cells were stimulated via Toll-like-receptor 9 (TLR-9) in the presence or absence of FTI. Regulatory functions, such as IL-10 and Granzyme B (GrB) secretion, were assessed by flow cytometry. In addition, effector B-cell functions, such as plasma cell formation and IgG secretion, were studied.

Results

The two FTI Lonafarnib and tipifarnib both suppressed TLR-9-induced B-cell proliferation. Maturation of IL-10 producing B-cells was suppressed by FTI at high concentrations as well as induction of GrB-secreting B-cells. Plasma blast formation and IgG secretion were potently suppressed by FTI. Moreover, purified B-cells from immunosuppressed renal transplant patients were also susceptible to FTI-induced suppression of effector functions, evidenced by diminished IgG secretion.

Conclusion

FTI suppress in vitro B-cell proliferation and plasma cell formation while partially preserving IL-10 as well as GrB production of B-cells. Thus, FTI may have immunosuppressive capacity encouraging further studies to investigate the potential immunomodulatory value of this agent.
2a Results: galaxy to cloud scales
Authors: Dobbs Clare
Published: November 2023

Simulations from the scales of isolated galaxies to clouds have been instrumental in informing us about molecular cloud formation and evolution. Simulations are able to investigate the roles of gravity, feedback, turbulence, heating and cooling, and magnetic fields on the physics of the interstellar medium, and star formation. Compared to simulations of individual clouds, galactic and sub-galactic scale simulations can include larger galactic scale processes such as spiral arms, bars, and larger supernovae bubbles, which may influence star formation. Simulations show cloud properties and lifetimes in broad agreement with observations. Gravity and spiral arms are required to produce more massive GMCs, whilst stellar feedback, likely photoionisation, leads to relatively short cloud lifetimes. On larger scales, supernovae may be more dominant in driving the structure and dynamics, but photoionisation may still have a role. In terms of the dynamics, feedback is probably the main driver of velocity dispersions, but large scale processes such as gravity and spiral arms may also be significant. Magnetic fields are generally found to decrease star formation on galaxy or cloud scales, and simulations are ongoing to study whether clouds are sub or supercritical on different scales in galaxy scale simulations. Simulations on subgalactic scales, or zoom in simulations, allow better resolution of feedback processes, filamentary structure within clouds, and the study of stellar clusters.
Sustainable agriculture and food sovereignty in Haiti: sharing knowledge and shaping understanding of food systems at the University of Fondwa
Authors: Joseph Lesly , Stephenson Max O. , Zanotti Laura , Ricot Scutt
Published: November 2023

The Association of Peasants of Fondwa (APF), a grassroots organization led by a visionary Haitian Spiritan priest, established the private nonprofit University of Fondwa (UNIF) in Haiti in 2004. The University aims to fill a gap in educational opportunities for rural youth and to develop community leaders able to steward food security, sustainable farm animal husbandry, and small business development. Since the institution’s foundation, University faculty members have explored low-input sustainable agriculture techniques, which were inspired by strategies shared earlier by Cuban agronomists and adapted to the Fondwa region’s mountainous terrain. While the University has faced and continues to confront many challenges related to its sustainability as an institution, this article describes the processes by which its faculty and students have conducted diagnoses of soils and crop choices, the innovations they have developed and introduced to improve harvest productivity in rural Haiti and, especially, the ways and means by which they have sought to share such (re)thinking of traditional practices with local farmers. We argue that the University of Fondwa faculty’s close collaboration with local farmers and the agricultural techniques they have refined thereby have not only improved food security for the families involved but have also contributed to the creation of social capital in the countryside and enabled participating Haitian farmers to imagine a path toward food sovereignty. In addition, by educating farmers and providing them tools to improve their food production, the University has worked to close the deep inequality gap that exists between urban and rural Haiti.
Biomechanical and tissue reaction: the effects of varying sutures size on canine abdominal wall stitching
Authors: Li Shuai , Guo Yizhe , Zhao Xingkai , Lang Dong , Zhou Zhenlei
Published: November 2023

Objective

Larger diameter sutures can provide sufficient tensile strength to surgical incisions but may exacerbate the inflammatory response caused by the amount of implanted foreign material. This experiment aims to investigate the differences in biomechanical stability and tissue reactivity after suturing canine midline abdominal incisions with different suture sizes.

Method

Assessing the biomechanical differences between USP 2-0, 3-0, and 4-0 PGA sutures using uniaxial tensile testing on ex vivo canine midline skin and fascial muscle tissues using either a simple continuous or simple interrupted technique. mRNA and protein expression levels of inflammatory factors were measured through RT-PCR and ELISA. Tissue reactivity was evaluated using a semi-quantitative scoring system.

Result

For strains below 30% in skin and below 50% in muscle, there were no significant differences among groups. The results of skin biomechanical testing showed that the USP 4-0 PGA suture group demonstrated significantly lower maximum tensile strength compared to the USP 2-0 PGA or USP 3-0 PGA suture groups. However, it remained capable of providing at least 56.3 N (1.03 MPa) tensile strength for canine skin incisions, matching the tensile strength requirements of general canine abdominal wall surgical incisions. In addition, there were no statistically significant differences observed in the maximum tensile strength among different size of sutures according to the data of biomechanical testing in muscle. Larger diameter sutures led to increased levels of inflammatory factors (IL-1β, IL-6, TNF-ɑ) and tissue reactivity. Simple interrupted sutures caused higher levels of inflammatory factors in muscular tissue compared to simple continuous sutures.

Conclusion

USP 4-0 PGA sutures provide sufficient biomechanical stability for suturing canine abdominal skin and linea alba. Suture size significantly influences tissue reactivity after suturing, with smaller gauge sutures reducing early tissue inflammatory response. Thus, USP 4-0 PGA suture has more advantages to suturing canine abdominal surgical incisions.
Mass and Force Lumping: An Essential Enhancement to the Intrinsic Beam Finite Element Discretization
Authors: Wang Jiachen , Zhou Zhou
Published: November 2023

This paper introduces the novel application of the mass and force lumping technique to enhance the finite element discretization of the fully intrinsic beam formulation. In our aeroelastic system model, 2-D unsteady aerodynamics were incorporated alongside simple calculations for thrust and gravity. Through the central difference discretization method, the discretized system was thoroughly examined, shedding light on the advantages of the mass and force lumping approach. With the use of a first-order lumping method, we successfully reconstructed the inertia matrices, external forces, and moments. The resulting equations are more systematically structured, facilitating the extraction of a regular state-space linear system using the direct index reduction method post-linearization. Numerical results further confirm that the proposed techniques can effectively capture the nonlinear dynamics of aeroelastic systems, enabling equation reconstruction and leading to significant benefits in system order reduction and flight dynamical analysis.
Comprehensive Gene Expression Profiling Analysis of Adipose Tissue in Male Individuals from Fat- and Thin-Tailed Sheep Breeds
Authors: Farhadi Sana , Hasanpur Karim , Ghias Jalil Shodja , Palangi Valiollah , Maggiolino Aristide , Landi Vincenzo
Published: November 2023

It has been shown that tail fat content varies significantly among sheep breeds and plays a significant role in meat quality. Recently, significant efforts have been made to understand the physiological, biochemical, and genomic regulation of fat deposition in sheep tails in order to unravel the mechanisms underlying energy storage and adipose tissue lipid metabolism. RNA-seq has enabled us to provide a high-resolution snapshot of differential gene expression between fat- and thin-tailed sheep breeds. Therefore, three RNA-seq datasets were meta-analyzed for the current work to elucidate the transcriptome profile differences between them. Specifically, we identified hub genes, performed gene ontology (GO) analysis, carried out enrichment analyses of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and validated hub genes using machine learning algorithms. This approach revealed a total of 136 meta-genes, 39 of which were not significant in any of the individual studies, indicating the higher statistical power of the meta-analysis. Furthermore, the results derived from the use of machine learning revealed POSTN, K35, SETD4, USP29, ANKRD37, RTN2, PRG4, and LRRC4C as substantial genes that were assigned a higher weight (0.7) than other meta-genes. Among the decision tree models, the Random Forest ones surpassed the others in adipose tissue predictive power fat deposition in fat- and thin-tailed breeds (accuracy > 0.85%). In this regard, combining meta-analyses and machine learning approaches allowed for the identification of three important genes (POSTN, K35, SETD4) related to lipid metabolism, and our findings could help animal breeding strategies optimize fat-tailed breeds’ tail sizes.
Optimization Strategies to Adapt Sheep Breeding Programs to Pasture-Based Production Environments: A Simulation Study
Authors: Martin Rebecca , Pook Torsten , Bennewitz Jörn , Schmid Markus
Published: November 2023

Strong differences between the selection (indoor fattening) and production environment (pasture fattening) are expected to reduce genetic gain due to possible genotype-by-environment interactions (G × E). To investigate how to adapt a sheep breeding program to a pasture-based production environment, different scenarios were simulated for the German Merino sheep population using the R package Modular Breeding Program Simulator (MoBPS). All relevant selection steps and a multivariate pedigree-based BLUP breeding value estimation were included. The reference scenario included progeny testing at stations to evaluate the fattening performance and carcass traits. It was compared to alternative scenarios varying in the progeny testing scheme for fattening traits (station and/or field). The total merit index (TMI) set pasture-based lamb fattening as a breeding goal, i.e., field fattening traits were weighted. Regarding the TMI, the scenario with progeny testing both in the field and on station led to a significant increase in genetic gain compared with the reference scenario. Regarding fattening traits, genetic gain was significantly increased in the alternative scenarios in which field progeny testing was performed. In the presence of G × E, the study showed that the selection environment should match the production environment (pasture) to avoid losses in genetic gain. As most breeding goals also contain traits not recordable in field testing, the combination of both field and station testing is required to maximize genetic gain.
Approach to Selective Dry Cow Therapy in Early Adopter Italian Dairy Farms: Why Compliance Is So Important
Authors: Guadagnini Marcello , Gogna Clarissa , Tolasi Cecilia , Tolasi Giacomo , Gnali Gisella , Freu Gustavo , Masroure Anoar Jamai , Moroni Paolo
Published: November 2023

Selective dry-cow therapy (SDCT) became mandatory in Italy on 28 January 2022. During 2020, a group of farms involved in a milk quality program began a pilot experiment with SDCT in order to understand its challenges and to identify areas for procedural improvements. The aim of this study was to describe the challenges and results of the SDCT in early adopters’ herds with a special focus on treatment compliance. Retrospective data from 1911 cows from 11 dairy herds were evaluated. Somatic cell counts, clinical mastitis (CM) history, and the California Mastitis Test (CMT) were used as criteria for SDCT. Based on the dairy herd improvement test results and CM history, 48% of all cows should have received antibiotic treatments and internal teat sealants. Adding the CMT at dry-off increased the percentage of antibiotic-treated cows to 62%, with relevant variation among farms. Concerning treatment compliance, 21% of the cows were “non-compliant”, suggesting the importance of monitoring treatment compliance. In conclusion, even if commonly used selection criteria for antibiotic treatments were used, the need for more education and in-depth monitoring of the SDCT adoption process was clearly identified. Close collaboration and agreement between veterinarians and farmers are key for SDCT adoption success.
Sign Language Studies with Chimpanzees in Sanctuary
Authors: Jensvold Mary Lee , Dombrausky Kailie , Collins Emily
Published: November 2023

Adult chimpanzees Tatu and Loulis lived at the Fauna Foundation sanctuary. They had acquired signs of American Sign Language (ASL) while young and continued to use them as adults. Caregivers with proficiency in ASL maintained daily sign language records during interactions and passive observation. Sign checklists were records of daily vocabulary use. Sign logs were records of signed interactions with caregivers and other chimpanzees. This study reports sign use from eight years of these records. Tatu and Loulis used a majority of their base vocabularies consistently over the study period. They used signs that they had acquired decades earlier and new signs. Their utterances served a variety of communicative functions, including responses, conversational devices, requests, and descriptions. They signed to caregivers, other chimpanzees, including those who did not use signs, and to themselves privately. This indicates the importance of a stimulating and interactive environment to understand the scope of ape communication and, in particular, their use of sign language.
Caffeine Administration in Piglets with Low Birthweight and Low Vitality Scores, and Its Effect on Physiological Blood Profile, Acid–Base Balance, Gas Exchange, and Infrared Thermal Response
Authors: Villanueva-García Dina , Ghezzi Marcelo , Mora-Medina Patricia , Hernández-Ávalos Ismael , Olmos-Hernández Adriana , Casas-Alvarado Alejandro , Lezama-García Karina , Domínguez-Oliva Adriana , Rodríguez-González Daniela , Marcet-Rius Miriam
Published: November 2023

Intrapartum asphyxia, fetal hypoxia, and their consequences (e.g., acidosis, hypercapnia, hypoglycemia, and hypothermia) are the main factors related to physio-metabolic imbalances that increase neonatal mortality in piglets, particularly in piglets with low birthweight and low vitality scores. This study aimed to evaluate the effect of three different doses of caffeine (10, 20, and 30 mg/kg) administered orally to 480 newborn piglets with low birthweight and low vitality scores. Blood gas parameters (pH, pO2, pCO2, and HCO3−), physio-metabolic profile (Ca++, glucose, and lactate), and the thermal response assessed through infrared thermography in four thermal windows (ocular, auricular, snout, and hindlimb) and rectal temperature were evaluated during the first 24 h of life. Doses of 30 mg/kg resulted in significant differences at 24 h for all evaluated parameters, suggesting that caffeine administration improved the cardiorespiratory function and metabolic activity of piglets by reducing acidosis, restoring glycemia, and increasing surface and rectal temperature. In conclusion, caffeine at 30 mg/kg could be suggested as an appropriate dose to use in piglets with low birthweight and low vitality scores. Future research might need to study the presentation of adverse effects due to higher caffeine concentrations.
Demonstration of SARS-CoV-2 Exposure in Korean Native Cattle and Korean Native Black Goats in Korea
Authors: Bae Da-Yun , Yang Ju-Hee , Moon Sung-Hyun , Kim Woo H. , Yoo Dae-Sung , Park Choi-Kyu , Shin Yeun-Kyung , Kang Hae-Eun , Tark Dongseob , Oh Yeonsu , Cho Ho-Seong
Published: November 2023

The COVID-19 pandemic is caused by the zoonotic SARS-CoV-2 virus. A wide range of animals that interact with humans have been investigated to identify potential infections. As the extent of infection became more apparent, extensive animal monitoring became necessary to assess their susceptibility. This study analyzed nasal swabs and blood samples collected from randomly selected Korean native cattle and Korean native black goats. The tests conducted included real-time qPCR to detect SARS-CoV-2 antigens, an ELISA to detect antibodies, and a plaque reduction neutralization test (PRNT) to determine the presence of neutralizing antibodies. Among the 1798 animals tested (consisting of 1174 Korean native cattle and 624 Korean native black goats), SARS-CoV-2 viral RNA was detected in one Korean native cattle and one Korean native black goat. ELISA testing revealed positive results for antibodies in 54 Korean native cattle (4.60%) and 16 Korean native black goats (2.56%), while PRNTs yielded positive results in 51 Korean native cattle (4.34%) and 14 Korean native black goats (2.24%). The presence of SARS-CoV-2 antigens and/or antibodies was identified in animals on farms where farmworkers were already infected. It is challenging to completely rule out the possibility of reverse zoonotic transmission from humans to livestock in Korea, although the transmission is not to the same extent as it is in highly susceptible animal species like minks, cats, and dogs. This is due to the limited geographical area and the dense, intensive farming practices implemented in these regions. In conclusion, continuous viral circulation between humans and animals is inevitable, necessitating ongoing animal monitoring to ensure public health and safety.
Cloud and Cloud Shadow Detection of GF-1 Images Based on the Swin-UNet Method
Authors: Tan Yuhao , Zhang Wenhao , Yang Xiufeng , Liu Qiyue , Mi Xiaofei , Li Juan , Yang Jian , Gu Xingfa
Published: November 2023

Cloud and cloud shadow detection in remote sensing images is an important preprocessing technique for quantitative analysis and large-scale mapping. To solve the problems of cloud and cloud shadow detection based on Convolutional Neural Network models, such as rough edges and insufficient overall accuracy, cloud and cloud shadow segmentation based on Swin-UNet was studied in the wide field of view (WFV) images of GaoFen-1 (GF-1). The Swin Transformer blocks help the model capture long-distance features and obtain deeper feature information in the network. This study selects a public GF1_WHU cloud and cloud shadow detection dataset for preprocessing and data optimization and conducts comparative experiments in different models. The results show that the algorithm performs well on vegetation, water, buildings, barren and other types. The average accuracy of cloud detection is 98.01%, the recall is 96.84% and the F1-score is 95.48%. The corresponding results of cloud shadow detection are 84.64%, 83.12% and 97.55%. In general, compared to U-Net, PSPNet and DeepLabV3+, this model performs better in cloud and cloud shadow detection, with clearer detection boundaries and a higher accuracy in complex surface conditions. This proves that Swin-UNet has great feature extraction capability in moderate and high-resolution remote sensing images.
Atmospheric Circulation Patterns during the Summertime Precipitation in Southeastern Iran
Authors: Mahoutchi Mohammad Hasan , Abbasi Esmaeil , Khoshakhlagh Faramarz , Rousta Iman , Olafsson Haraldur , Baranowski Piotr , Krzyszczak Jaromir
Published: November 2023

The accurate recognition of atmospheric circulation patterns is vital for understanding the intricate relationships among various climatic elements. Therefore, the main goal of this study is to comprehensively identify circulation patterns during the occurrence of the summertime Extended Area Precipitation Event (EAPE) in southeastern Iran. The data used in this study encompass precipitation rates from synoptic and rain gauge stations, Geopotential Height (GPH), omega (upward motion speed), u-wind (east-west), and v-wind (north-south) components at different atmospheric levels, along with satellite images from the visible spectrum. In this research, both subjective and objective clustering methods have been utilized to identify synoptic circulation patterns based on 500-hPa GPH data. Summer precipitation was chosen for analysis because its characteristics and relationships with large-scale circulation patterns are less understood compared to those of winter precipitation. Examination of the 500-hPa GPH data for sixty-two identified cases of EAPE over southeast Iran revealed that the causative factors for these events are comprised of five recurring patterns (referred to here for convenience as AP, BP, CP, DP, and EP). Three of these patterns (AP, BP, and DP) significantly contributed to 71% of all EAPE cases. It was evident that the five patterns responsible for creating the EAPE in southeastern Iran had distinct directions.
Comprehensive Study of Failure Mechanisms of Field-Aged Automotive Lead Batteries
Authors: Conradt Rafael , Schröer Philipp , Dazer Martin , Wirth Jonathan , Jöris Florian , Schulte Dominik , Birke Kai Peter
Published: November 2023

Modern vehicles have increasing safety requirements and a need for reliable low-voltage power supply in their on-board power supply systems. Understanding the causes and probabilities of failures in a 12 V power supply is crucial. Field analyses of aged and failed 12 V lead batteries can provide valuable insights regarding this topic. In a previous study, non-invasive electrical testing was used to objectively determine the reasons for failure and the lifetime of individual batteries. By identifying all of the potential failure mechanisms, the Latin hypercube sampling method was found to effectively reduce the required sample size. To ensure sufficient confidence in validating diagnostic algorithms and calculating time-dependent failure rates, all identified aging phenomena must be considered. This study presents a probability distribution of the failure mechanisms that occur in the field, as well as provides insights into potential opportunities, but it also challenges diagnostic approaches for current and future vehicles.
Possibilities for Utilization of Cherry Products (Juice and Pomace) in Beer Production
Authors: Nedyalkov Petar , Bakardzhiyski Ivan , Shikov Vasil , Kaneva Maria , Shopska Vesela
Published: November 2023

Fruit addition can enrich beer with flavor and bioactive substances. Sweet cherry (Prunus avium L.) can be added in beer as a whole fruit, fruit juice, or pulp, but there is no data for the addition of cherry pomace in beer. Therefore, we investigated the addition of cherry juice and pomace during beer fermentation on the first and seventh day and studied the basic beer parameters (alcohol and extract), sensorial evaluation, phenolic compounds, and antioxidant activity of the beers produced, measured using six different methods (DPPH, ABTS, FRAP, CUPRAC, ORAC, and HORAC) and compared the results with a control sample without cherry products addition. The results showed a strong correlation between the antioxidant activity values obtained using the DPPH, FRAP, CUPRAC, and HORAC methods and the concentration of phenolic compounds in the studied beers. The phenolic compound content and antioxidant activity increased when cherries juice or pomace were added. The increase was much more significant when pomace was used. Therefore, it can be concluded that cherry pomace addition is a better option than cherry juice for beer production because of the increased content of bioactive compounds and the sustainability of the beers obtained.
Development and Evaluation of a Natural Language Processing System for Curating a Trans-Thoracic Echocardiogram (TTE) Database
Authors: Dong Tim , Sunderland Nicholas , Nightingale Angus , Fudulu Daniel P. , Chan Jeremy , Zhai Ben , Freitas Alberto , Caputo Massimo , Dimagli Arnaldo , Mires Stuart , Wyatt Mike , Benedetto Umberto , Angelini Gianni D.
Published: November 2023

Background: Although electronic health records (EHR) provide useful insights into disease patterns and patient treatment optimisation, their reliance on unstructured data presents a difficulty. Echocardiography reports, which provide extensive pathology information for cardiovascular patients, are particularly challenging to extract and analyse, because of their narrative structure. Although natural language processing (NLP) has been utilised successfully in a variety of medical fields, it is not commonly used in echocardiography analysis. Objectives: To develop an NLP-based approach for extracting and categorising data from echocardiography reports by accurately converting continuous (e.g., LVOT VTI, AV VTI and TR Vmax) and discrete (e.g., regurgitation severity) outcomes in a semi-structured narrative format into a structured and categorised format, allowing for future research or clinical use. Methods: 135,062 Trans-Thoracic Echocardiogram (TTE) reports were derived from 146967 baseline echocardiogram reports and split into three cohorts: Training and Validation (n = 1075), Test Dataset (n = 98) and Application Dataset (n = 133,889). The NLP system was developed and was iteratively refined using medical expert knowledge. The system was used to curate a moderate-fidelity database from extractions of 133,889 reports. A hold-out validation set of 98 reports was blindly annotated and extracted by two clinicians for comparison with the NLP extraction. Agreement, discrimination, accuracy and calibration of outcome measure extractions were evaluated. Results: Continuous outcomes including LVOT VTI, AV VTI and TR Vmax exhibited perfect inter-rater reliability using intra-class correlation scores (ICC = 1.00, p < 0.05) alongside high R2 values, demonstrating an ideal alignment between the NLP system and clinicians. A good level (ICC = 0.75–0.9, p < 0.05) of inter-rater reliability was observed for outcomes such as LVOT Diam, Lateral MAPSE, Peak E Velocity, Lateral E’ Velocity, PV Vmax, Sinuses of Valsalva and Ascending Aorta diameters. Furthermore, the accuracy rate for discrete outcome measures was 91.38% in the confusion matrix analysis, indicating effective performance. Conclusions: The NLP-based technique yielded good results when it came to extracting and categorising data from echocardiography reports. The system demonstrated a high degree of agreement and concordance with clinician extractions. This study contributes to the effective use of semi-structured data by providing a useful tool for converting semi-structured text to a structured echo report that can be used for data management. Additional validation and implementation in healthcare settings can improve data availability and support research and clinical decision-making.
Plant Cellulose as a Substrate for 3D Neural Stem Cell Culture
Authors: Couvrette Lauren J. , Walker Krystal L. A. , Bui Tuan V. , Pelling Andrew E.
Published: November 2023

Neural stem cell (NSC)-based therapies are at the forefront of regenerative medicine strategies for various neural defects and injuries such as stroke, traumatic brain injury, and spinal cord injury. For several clinical applications, NSC therapies require biocompatible scaffolds to support cell survival and to direct differentiation. Here, we investigate decellularized plant tissue as a novel scaffold for three-dimensional (3D), in vitro culture of NSCs. Plant cellulose scaffolds were shown to support the attachment and proliferation of adult rat hippocampal neural stem cells (NSCs). Further, NSCs differentiated on the cellulose scaffold had significant increases in their expression of neuron-specific beta-III tubulin and glial fibrillary acidic protein compared to 2D culture on a polystyrene plate, indicating that the scaffold may enhance the differentiation of NSCs towards astrocytic and neuronal lineages. Our findings suggest that plant-derived cellulose scaffolds have the potential to be used in neural tissue engineering and can be harnessed to direct the differentiation of NSCs.
Early Osteogenic-Induced Adipose-Derived Stem Cells and Canine Bone Regeneration Potential Analyzed Using Biodegradable Scaffolds
Authors: Yun Hyun-Ho , Kim Seong-Gon , Park Se-Il , Jo Woori , Kang Kyung-Ku , Lee Eun-Joo , Kim Dong-Kyu , Jung Hoe-Su , Son Ji-Yoon , Park Jae-Min , Park Hyun-Sook , Lee Sunray , Shin Hong-In , Hong Il-Hwa , Jeong Kyu-Shik
Published: November 2023

The complex process of bone regeneration is influenced by factors such as inflammatory responses, tissue interactions, and progenitor cells. Currently, multiple traumas can interfere with fracture healing, causing the prolonging or failure of healing. In these cases, bone grafting is the most effective treatment. However, there are several drawbacks, such as morbidity at the donor site and availability of suitable materials. Advantages have been provided in this field by a variety of stem cell types. Adipose-derived stem cells (ASCs) show promise. In the radiological examination of this study, it was confirmed that the C/S group showed faster regeneration than the other groups, and Micro-CT also showed that the degree of bone formation in the defect area was highest in the C/S group. Compared to the control group, the change in cortical bone area in the defect area decreased in the sham group (0.874), while it slightly increased in the C/S group (1.027). An increase in relative vascularity indicates a decrease in overall bone density, but a weak depression filled with fibrous tissue was observed outside the compact bone. It was confirmed that newly formed cortical bone showed a slight difference in bone density compared to surrounding normal bone tissue due to increased distribution of cortical bone. In this study, we investigated the effect of bone regeneration by ADMSCs measured by radiation and pathological effects. These data can ultimately be applied to humans with important clinical applications in various bone diseases, regenerative, and early stages of formative differentiation.
Antiproliferative Effects in Colorectal Cancer and Stabilisation in Cyclodextrins of the Phytoalexin Isorhapontigenin
Authors: Navarro-Orcajada Silvia , Vidal-Sánchez Francisco José , Conesa Irene , Escribano-Naharro Francisco , Matencio Adrián , López-Nicolás José Manuel
Published: November 2023

Isorhapontigenin has been proposed as a better alternative for oral administration than the famous resveratrol, as it shares many biological activities, but with a structure that could make its delivery easier. Although this hydrophobic structure could enhance bioavailability, it could also be a disadvantage in the development of products. In this research, we study the antiproliferative activity of this stilbene against colorectal cancer and overcome its limitations through molecular encapsulation in cyclodextrins. The cytotoxic activity against human colorectal cancer cells of isorhapontigenin was similar to that of resveratrol or piceatannol, supporting its use as a bioactive alternative. The study of the encapsulation through fluorescence spectroscopy and molecular docking revealed that the complexation satisfies a 1:1 stoichiometry and that HP-β-CD is the most suitable CD to encapsulate this stilbene. Through a spectrophotometric assay, it was observed that this CD could double the basal water solubility, exceeding the solubility of other hydroxylated stilbenes. The stability of these inclusion complexes was higher at a pH below 9 and refrigeration temperatures. Moreover, the use of CDs retained more than 78% of isorhapontigenin after storage for 12 weeks, compared to 15% in free form. Overall, these findings could help design novel formulations to better deliver isorhapontigenin.
Impacts of Chemerin Levels and Antioxidant Capacity on the Severity of Cardiovascular Autonomic Neuropathy in Patients with Type 2 Diabetes and Prediabetes
Authors: Lai Yun-Ru , Huang Chih-Cheng , Cheng Ben-Chung , Chiu Wen-Chan , Lin Ting-Yin , Chiang Hui-Ching , Kuo Chun-En , Lu Cheng-Hsien
Published: November 2023

Existing evidence supports an association between chemerin levels and cardiovascular risk, while reduced thiol levels are linked to diabetes mellitus. It is hypothesized that chemerin may contribute to autonomic dysfunction and cardiovascular risk in type 2 diabetes mellitus (T2DM), potentially mediated by the antioxidant capacity of patients with well-controlled T2DM and prediabetes. Comprehensive cardiovascular autonomic testing and biomarker assessments were conducted for all participants. The severity of cardiovascular autonomic neuropathy (CAN) was evaluated using the composite autonomic scoring scale (CASS). A mediation model was employed to explore the potential relationships among chemerin levels, antioxidant capacity (indicated by thiol levels), and CAN severity (indicated by CASS values). A total of 184 participants were enrolled in this study, comprising 143 individuals with T2DM and 40 individuals with prediabetes. The findings reveal a significant negative association between thiols levels (r = −0.38, p < 0.0001) and the CASS values, while a positive association is observed between chemerin levels (r = 0.47, p < 0.0001) and the CASS values. Linear regression analysis identified chemerin and thiols as independent variables significantly associated with CASS values. Subsequent mediation analysis elucidated that thiols levels act as mediators in the relationship between elevated chemerin levels and an increased CASS value. This study shows that poor cardiovascular function, higher chemerin levels, and reduced antioxidant capacity coexist in individuals with T2DM and prediabetes. Mediation analysis suggests a pathophysiological link between high chemerin levels and low antioxidant capacity, adversely impacting CAN severity.
Inhibiting CBP Decreases AR Expression and Inhibits Proliferation in Benign Prostate Epithelial Cells
Authors: Tang Xingxing , Liu Zhifu , Li Zheng , Huang Chenchen , Yu Wei , Fan Yu , Hu Shuai , Jin Jie
Published: November 2023

(1) Background: CREB-binding protein (CBP) is a key transcriptional coactivator of androgen receptors (AR). We conducted this study to investigate the effects of CBP on AR expression and proliferation in benign prostatic hyperplasia (BPH) prostate epithelial cells. (2) Methods: By analyzing a published data set, we found that CBP was closely related to the gene expression of AR in prostate cells. We enrolled 20 BPH patients who underwent transurethral resection of the prostate (TURP) in Peking University First Hospital in 2022, and analyzed the expressions of CBP and AR in BPH prostate tissues. Then, we used ICG-001 and shRNA to inhibit CBP in prostate epithelial cells (BPH-1 cells and RWPE-1 cells), and conducted immunofluorescence, cell viability assay, flow cytometry analysis, and Western blot to analyze the effects of CBP on AR expression and proliferation in prostate epithelial cells. We also studied the interaction between CBP and AR through a co-immunoprecipitation assay. (3) Results: CBP is consistent with AR in expression intensity in prostate tissues. Inhibiting CBP decreases AR expression, and induces proliferation inhibition, apoptosis, and cell cycle arrest in BPH prostate epithelial cells. The co-immunoprecipitation assay showed that CBP binds with AR to form transcription complexes in prostate epithelial cells. (4) Conclusions: Inhibiting CBP decreases AR expression and inhibits proliferation in benign prostate epithelial cells. CBP may be a potential target to affect AR expression and the proliferation of prostate epithelial cells in BPH.