bluuebunny/crossref_metadata_2025 huggingface dataset:
Resveratrol Protects against Helicobacter pylori-Associated Gastritis by Combating Oxidative Stress
Authors: Zhang Xiaolin , Jiang Anmin , Qi Banghua , Ma Zhongyou , Xiong Youyi , Dou Jinfeng , Wang Jianfei
Published: November 2015

Helicobacter pylori (H. pylori)-induced oxidative stress has been shown to play a very important role in the inflammation of the gastric mucosa and increases the risk of developing gastric cancer. Resveratrol has many biological functions and activities, including antioxidant and anti-inflammatory effect. The purpose of this study was to probe whether resveratrol inhibits H. pylori-induced gastric inflammation and to elucidate the underlying mechanisms of any effect in mice. A mouse model of H. pylori infection was established via oral inoculation with H. pylori. After one week, mice were administered resveratrol (100 mg/kg body weight/day) orally for six weeks. The mRNA and protein levels of iNOS and IL-8 were assessed using RT-PCR, Western blot and ELISA. The expression levels of IκBα and phosphorylated IκBα (which embodies the level and activation of NF-κB), Heme Oxygenase-1 (HO-1; a potent antioxidant enzyme) and nuclear factor-erythroid 2 related factor 2 (Nrf2) were determined using Western blot, and lipid peroxide (LPO) level and myeloperoxidase (MPO) activity were examined using an MPO colorimetric activity assay, thiobarbituric acid reaction, and histological-grade using HE staining of the gastric mucosa. The results showed that resveratrol improved the histological infiltration score and decreased LPO level and MPO activity in the gastric mucosa. Resveratrol down-regulated the H. pylori-induced mRNA transcription and protein expression levels of IL-8 and iNOS, suppressed H. pylori-induced phosphorylation of IκBα, and increased the levels of HO-1 and Nrf2. In conclusion, resveratrol treatment exerted significant effects against oxidative stress and inflammation in H. pylori-infected mucosa through the suppression of IL-8, iNOS, and NF-κB, and moreover through the activation of the Nrf2/HO-1 pathway.
The Deubiquitinase USP17 Regulates the Stability and Nuclear Function of IL-33
Authors: Ni Yingmeng , Tao Lianqin , Chen Chen , Song Huihui , Li Zhiyuan , Gao Yayi , Nie Jia , Piccioni Miranda , Shi Guochao , Li Bin
Published: November 2015

IL-33 is a new member of the IL-1 family cytokines, which is expressed by different types of immune cells and non-immune cells. IL-33 is constitutively expressed in the nucleus, where it can act as a transcriptional regulator. So far, no direct target for nuclear IL-33 has been identified, and the regulation of IL-33 nuclear function remains largely unclear. Here, we report that the transcription of type 2 inflammatory cytokine IL-13 is positively regulated by nuclear IL-33. IL-33 can directly bind to the conserved non-coding sequence (CNS) before the translation initiation site in the IL13 gene locus. Moreover, IL-33 nuclear function and stability are regulated by the enzyme ubiquitin-specific protease 17 (USP17) through deubiquitination of IL-33 both at the K48 and at the K63 sites. Our data suggest that IL13 gene transcription can be directly activated by nuclear IL-33, which is negatively regulated by the deubiquitinase USP17.
The kin17 Protein in Murine Melanoma Cells
Authors: Ramos Anelise , Gaspar Vanessa , Kelmer Sabrina , Sellani Tarciso , Batista Ana , De Lima Neto Quirino , Rodrigues Elaine , Fernandez Maria
Published: November 2015

kin17 has been described as a protein involved in the processes of DNA replication initiation, DNA recombination, and DNA repair. kin17 has been studied as a potential molecular marker of breast cancer. This work reports the detection and localization of this protein in the murine melanoma cell line B16F10-Nex2 and in two derived subclones with different metastatic potential, B16-8HR and B16-10CR. Nuclear and chromatin-associated protein fractions were analyzed, and kin17 was detected in all fractions, with an elevated concentration observed in the chromatin-associated fraction of the clone with low metastatic potential, suggesting that the kin17 expression level could be a marker of melanoma.
Neonatal Screening for Medium-Chain Acyl-CoA Deficiency—Insights and Unexpected Challenges
Authors: Maier Esther
Published: November 2015

With the implementation of tandem mass spectrometry (MS/MS), neonatal screening for medium-chain acyl-CoA dehydrogenase (MCADD) has been introduced in many screening programs worldwide. Together with phenylketonuria, MCADD is the disorder most frequently diagnosed. Despite undeniable beneficial effects on morbidity and mortality, neonatal screening for MCADD effectively exemplifies the unexpected challenges of increased diagnosis by screening programs. MS/MS-based screening revealed an at least 2-fold higher incidence than expected with a considerable share of individuals showing mild biochemical alterations and/or novel mutations with unknown clinical significance. Whether these individuals are at lower risk to experience metabolic decompensations is a matter of ongoing debate. Defining patients, stratifying them according to their clinical risk, and adopting treatment protocols is an as yet unmet challenge in neonatal screening for MCADD.
Evaluation of Cyantraniliprole and Other Commercial Fly Baits under Laboratory and Field Conditions
Authors: Parker Casey , Baldwin Rebecca , Pereira Roberto , Koehler Philip
Published: November 2015

Laboratory and field trials were performed to evaluate the attractiveness and efficacy of commercial baits (cyantraniliprole; methomyl + (Z)-9-tricosene; dinotefuran + (Z)-9-tricosene; imidacloprid granular + (Z)-9-tricosene; and imidacloprid liquid + (Z)-9-tricosene). In choice tests; flies were most attracted to cyantraniliprole bait > dinotefuran + (Z)-9 > methomyl + (Z)-9 bait > imidacloprid granular + (Z)-9 bait > imidacloprid liquid + (Z)-9 bait. Significant degradation in bait efficacy was observed after two weeks of aging excluding imidacloprid granular; which began to degrade in field conditions after one week. Cyantraniliprole; the new fly bait active ingredient in Zyrox®; had the longest time to knockdown in the laboratory tests; but on susceptible flies; achieved 95%–100% knockdown within an hour of exposure. Zyrox® was resistant to weathering for a week; and was more attractive to flies in the field when compared to methomyl + (Z)-9 bait.
Pigment Epithelium-Derived Factor (PEDF) is a Determinant of Stem Cell Fate: Lessons from an Ultra-Rare Disease
Authors: Sagheer Usman , Gong Jingjing , Chung Chuhan
Published: November 2015

PEDF is a secreted glycoprotein that is widely expressed by multiple organs. Numerous functional contributions have been attributed to PEDF with antiangiogenic, antitumor, anti-inflammatory, and neurotrophic properties among the most prominent. The discovery that null mutations in the PEDF gene results in Osteogenesis Imperfecta Type VI, a rare autosomal recessive bone disease characterized by multiple fractures, highlights a critical developmental function for this protein. This ultra-rare orphan disease has provided biological insights into previous studies that noted PEDF’s effects on various stem cell populations. In addition to bone development, PEDF modulates resident stem cell populations in the brain, muscle, and eye. Functional effects on human embryonic stem cells have also been demonstrated. An overview of recent advances in our understanding by which PEDF regulates stem cells and their potential clinical applications will be evaluated in this review.
Towards a Guidance System to Aid in the Dosimetry Calculation of Intraoperative Electron Radiation Therapy
Authors: Portalés Cristina , Gimeno Jesús , Vera Lucía , Fernández Marcos
Published: November 2015

In Intraoperative Electron Radiation Therapy (IOERT), the lack of specific planning tools limits its applicability, the need for accurate dosimetry estimation and application during the therapy being the most critical. Recently, some works have been presented that try to overcome some of the limitations for establishing planning tools, though still an accurate guidance system that tracks, in real-time, the applicator and the patient is needed. In these surgical environments, the acquisition of an accurate 3D shape of the patient’s tumor bed in real-time is of high interest, as current systems make use of a 3D model acquired before the treatment. In this paper, an optical-based system is presented that is able to register, in real-time, the different objects (rigid objects) taking part in such a treatment. The presented guidance system and the related methodology are highly interactive, where a usability design has also been provided for non-expert users. Additionally, four different approaches are explored and evaluated to acquire the 3D model of the patient (non-rigid object) in real-time, where accuracies in the range of 1 mm can be achieved without the need of using expensive devices.
Time Evolution of Man-Made Harbor Modifications in San Diego: Effects on Tsunamis
Authors: Barberopoulou Aggeliki , Legg Mark , Gica Edison
Published: November 2015

San Diego, one of the largest ports on the U.S. West Coast and home to the largest U.S. Navy base, is exposed to various local and distant tsunami sources. During the first half of the twentieth century, extensive modifications to the port included but were not limited to dredging, expansion of land near the airport and previous tidal flats, as well as creation of jetties. Using historical nautical charts and available Digital Elevation Models, this study gives an overview of changes to San Diego harbor in the last 150+ years due to human intervention and examines the effects of these changes on tsunamis. Two distant and two local scenarios were selected to demonstrate the impact of modified nearshore topography and bathymetry to incoming tsunamis. Inundation pattern, flow depths, and flooded localities vary greatly from year to year in the four scenarios. Specifically, flooded areas shift from the inner harbor to outer locations. Currents induced by the distant tsunamis intensify with modifications and shift from locations primarily outside the harbor to locations inside. A new characteristic in tsunami dynamics associated with port modifications is the introduction of high current spots. Numerical results also show that the introduction of high currents could threaten navigation, vessels, and facilities at narrow openings and also along the harbor “throat”—therefore, at an increased number of locations. Modifications in the port show that changes could have a negative but also a positive impact through constraint of flooding outside of the harbor and shifting of high currents to locations of minimal impact. The results of this study may be used as a first step toward future harbor design plans to reduce tsunami damages.
Soil Organic Carbon in Mangrove Ecosystems with Different Vegetation and Sedimentological Conditions
Authors: Matsui Naohiro , Meepol Wijarn , Chukwamdee Jirasak
Published: November 2015

A large number of studies have been conducted on organic carbon (OC) variation in mangrove ecosystems. However, few have examined its relationship with soil quality and stratigraphic condition. Mangrove OC characteristics would be explicitly understood if those two parameters were taken into account. The aim of this study was to examine mangrove OC characteristics qualitatively and quantitatively after distinguishing mangrove OC from other OC. Geological survey revealed that the underground of a mangrove ecosystem was composed of three layers: a top layer of mangrove origin and two underlying sublayers of geologic origin. The underlying sublayers were formed from different materials, as shown by X-ray fluorescence analysis. Despite a large thickness exceeding 700 cm in contrast to the 100 cm thickness of the mangrove mud layer, the sublayers had much lower OC stock. Mangrove mud layer formation started from the time of mangrove colonization, which dated back to between 1330 and 1820 14C years BP, and OC stock in the mangrove mud layer was more than half of the total OC stock in the underground layers, which had been accumulating since 7200 14C years BP. pH and redox potential (Eh) of the surface soils varied depending on vegetation type. In the surface soils, pH correlated to C% (r = −0.66, p < 0.01). C/N ratios varied widely from 3.9 to 34.3, indicating that mangrove OC had various sources. The pH and Eh gradients were important factors affecting the OC stock and the mobility/uptake of chemical elements in the mangrove mud layer. Humic acids extracted from the mangrove mud layer had relatively high aliphatic contents, in contrast with the carboxylic acid rich sublayers, indicating that humification has not yet progressed in mangrove soil.
Patterns of Wastewater Infrastructure along a Gradient of Coastal Urbanization: A Study of the Puget Sound Region
Authors: Spirandelli Daniele
Published: November 2015

The aim of this paper is to explore patterns of wastewater infrastructures (sewers vs. septic tanks) in urbanizing watersheds across a coastal metropolitan region. This research combines an urban-rural gradient with spatial metrics at the patch and watershed scale (proportion of parcels on a treatment system, septic density, lot size and percent imperviousness) to analyze wastewater patterns in the Puget Sound, WA, USA. Results show that most urban residential parcels are hooked up to a sewer, although there remain urban residences on a septic tank with small lots. I find a complex arrangement of wastewater treatment in suburban watersheds representing a patchwork of parcels on sewers and septic tanks. Sewers dominate in total numbers, while the density of septic tanks is highest in this portion of the urban gradient. Lot size decreases from rural to urban; however, it varies depending on the type of wastewater treatment system. In urban watersheds, lots on septic tanks are significantly smaller than lots in suburban and rural watersheds and of a similar size compared to lots on sewers. I also find a significant difference in the amount of impervious surfaces in watersheds dominated by sewers vs. septic tanks. In the urban portion of the gradient, the amount of paved surfaces in parcels with septic tanks is also similar in level as parcels with sewers. I discuss how these patterns emerge from the interplay of biophysical, socio-economic and technological factors and how different regulatory regimes for septic tanks and sewers may further induce these patterns.
(8R,10aS)-Methyl 2,4-diamino-8-(tert-butyl)-6-oxo-6,8,10,10a-tetrahydrooxazolo[3′′,4′′:1′,5′]-pyrrolo[3′,4′:5,6]pyrido[2,3-d]pyrimidine-10a-carboxylate
Authors: Clarke Georgia , Moloney Mark
Published: November 2015

A fused polyheterocyclic derivative is available by annulation of a tetramate scaffold, and has been shown to have some Gram-negative, but not Gram-positive, antibacterial activity.
1,4-Di(2-butoxycarbonyl-trans-vinyl)-2,5-dimethoxybenzene
Authors: Molano William , Sierra Cesar , Ochoa-Puentes Cristian
Published: November 2015

The title compound 1,4-di(2-butoxycarbonyl-trans-vinyl)-2,5-dimethoxybenzene was synthesized in 94% yield through the Heck reaction between 2,5-diiodo-1,4-dimethoxybenzene and n-butyl acrylate, using bis(dibenzylideneacetone) palladium(0) as homogeneous catalyst.
N′-(1,3-Dithiolan-2-ylidene)-3-(phenylamino)propanehydrazide
Authors: Tumosienė Ingrida , Kantminienė Kristina , Beresnevičius Zigmuntas
Published: November 2015

A synthesis of N′-(1,3-dithiolan-2-ylidene)-3-(phenylamino)propanehydrazide from 3-(phenylamino)propanehydrazide, carbon disulfide and 1,2-dibromoethane is reported. The title compound was characterized by 1H NMR, 13C NMR, ESI/MS, and elemental analysis.
Methyl (6-Ethyldibenzo[d,f][1,3]dioxepin-6-yl)acetate
Authors: Fattuoni Claudia , Palmas Francesco
Published: November 2015

Starting from 2,2'-dihydroxybiphenyl and an allenic ester, the title dibenzo-condensed dioxepine derivative was synthesized using a one-pot procedure.
Hydrogenation Properties of TiFe Doped with Zirconium
Authors: Gosselin Catherine , Huot Jacques
Published: November 2015

The goal of this study was to optimize the activation behaviour of hydrogen storage alloy TiFe. We found that the addition of a small amount of Zr in TiFe alloy greatly reduces the hydrogenation activation time. Two different procedural synthesis methods were applied: co-melt, where the TiFe was melted and afterward re-melted with the addition of Zr, and single-melt, where Ti, Fe and Zr were melted together in one single operation. The co-melted sample absorbed hydrogen at its maximum capacity in less than three hours without any pre-treatment. The single-melted alloy absorbed its maximum capacity in less than seven hours, also without pre-treatment. The reason for discrepancies between co-melt and single-melt alloys was found to be the different microstructure. The effect of air exposure was also investigated. We found that the air-exposed samples had the same maximum capacity as the argon protected samples but with a slightly longer incubation time, which is probably due to the presence of a dense surface oxide layer. Scanning electron microscopy revealed the presence of a rich Zr intergranular phase in the TiFe matrix, which is responsible for the enhanced hydrogenation properties of these Zr-doped TiFe alloys.
Chiral Nematic Structure of Cellulose Nanocrystal Suspensions and Films; Polarized Light and Atomic Force Microscopy
Authors: Gray Derek , Mu Xiaoyue
Published: November 2015

Cellulosic liquid crystalline solutions and suspensions form chiral nematic phases that show a rich variety of optical textures in the liquid crystalline state. These ordered structures may be preserved in solid films prepared by evaporation of solvent or suspending medium. Film formation from aqueous suspensions of cellulose nanocrystals (CNC) was investigated by polarized light microscopy, optical profilometry and atomic force microscopy (AFM). An attempt is made to interpret qualitatively the observed textures in terms of the orientation of the cellulose nanocrystals in the suspensions and films, and the changes in orientation caused by the evaporative process. Mass transfer within the evaporating droplet resulted in the formation of raised rings whose magnitude depended on the degree of pinning of the receding contact line. AFM of dry films at short length scales showed a radial orientation of the CNC at the free surface of the film, along with a radial height variation with a period of approximately P/2, ascribed to the anisotropic shrinkage of the chiral nematic structure.
Mechanisms of in Vivo Degradation and Resorption of Calcium Phosphate Based Biomaterials
Authors: Sheikh Zeeshan , Abdallah Mohamed-Nur , Hanafi Ahmed , Misbahuddin Syed , Rashid Haroon , Glogauer Michael
Published: November 2015

Calcium phosphate ceramic materials are extensively used for bone replacement and regeneration in orthopedic, dental, and maxillofacial surgical applications. In order for these biomaterials to work effectively it is imperative that they undergo the process of degradation and resorption in vivo. This allows for the space to be created for the new bone tissue to form and infiltrate within the implanted graft material. Several factors affect the biodegradation and resorption of calcium phosphate materials after implantation. Various cell types are involved in the degradation process by phagocytic mechanisms (monocytes/macrophages, fibroblasts, osteoblasts) or via an acidic mechanism to reduce the micro-environmental pH which results in demineralization of the cement matrix and resorption via osteoclasts. These cells exert their degradation effects directly or indirectly through the cytokine growth factor secretion and their sensitivity and response to these biomolecules. This article discusses the mechanisms of calcium phosphate material degradation in vivo.
Selectivity of Direct Methanol Fuel Cell Membranes
Authors: Aricò Antonino , Sebastian David , Schuster Michael , Bauer Bernd , D'Urso Claudia , Lufrano Francesco , Baglio Vincenzo
Published: November 2015

Sulfonic acid-functionalized polymer electrolyte membranes alternative to Nafion® were developed. These were hydrocarbon systems, such as blend sulfonated polyetheretherketone (s-PEEK), new generation perfluorosulfonic acid (PFSA) systems, and composite zirconium phosphate–PFSA polymers. The membranes varied in terms of composition, equivalent weight, thickness, and filler and were investigated with regard to their methanol permeation characteristics and proton conductivity for application in direct methanol fuel cells. The behavior of the membrane electrode assemblies (MEA) was investigated in fuel cell with the aim to individuate a correlation between membrane characteristics and their performance in a direct methanol fuel cell (DMFC). The power density of the DMFC at 60 °C increased according to a square root-like function of the membrane selectivity. This was defined as the reciprocal of the product between area specific resistance and crossover. The power density achieved at 60 °C for the most promising s-PEEK-based membrane-electrode assembly (MEA) was higher than the benchmark Nafion® 115-based MEA (77 mW·cm−2 vs. 64 mW·cm−2). This result was due to a lower methanol crossover (47 mA·cm−2 equivalent current density for s-PEEK vs. 120 mA·cm−2 for Nafion® 115 at 60 °C as recorded at OCV with 2 M methanol) and a suitable area specific resistance (0.15 Ohm cm2 for s-PEEK vs. 0.22 Ohm cm2 for Nafion® 115).
Integrating Colon Cancer Microarray Data: Associating Locus-Specific Methylation Groups to Gene Expression-Based Classifications
Authors: Barat Ana , Ruskin Heather , Byrne Annette , Prehn Jochen
Published: November 2015

Recently, considerable attention has been paid to gene expression-based classifications of colorectal cancers (CRC) and their association with patient prognosis. In addition to changes in gene expression, abnormal DNA-methylation is known to play an important role in cancer onset and development, and colon cancer is no exception to this rule. Large-scale technologies, such as methylation microarray assays and specific sequencing of methylated DNA, have been used to determine whole genome profiles of CpG island methylation in tissue samples. In this article, publicly available microarray-based gene expression and methylation data sets are used to characterize expression subtypes with respect to locus-specific methylation. A major objective was to determine whether integration of these data types improves previously characterized subtypes, or provides evidence for additional subtypes. We used unsupervised clustering techniques to determine methylation-based subgroups, which are subsequently annotated with three published expression-based classifications, comprising from three to six subtypes. Our results showed that, while methylation profiles provide a further basis for segregation of certain (Inflammatory and Goblet-like) finer-grained expression-based subtypes, they also suggest that other finer-grained subtypes are not distinctive and can be considered as a single subtype.
Numerical Analysis and Experimental Study of Hard Roofs in Fully Mechanized Mining Faces under Sleeve Fracturing
Authors: Zheng Zhitao , Xu Ying , Li Desheng , Dong Jianghui
Published: November 2015

Sudden falls of large-area hard roofs in a mined area release a large amount of elastic energy, generate dynamic loads, and cause disasters such as impact ground pressure and gas outbursts. To address these problems, in this study, the sleeve fracturing method (SFM) was applied to weaken a hard roof. The numerical simulation software FLAC3D was used to develop three models based on an analysis of the SFM working mechanism. These models were applied to an analysis of the fracturing effects of various factors such as the borehole diameter, hole spacing, and sleeve pressure. Finally, the results of a simulation were validated using experiments with similar models. Our research indicated the following: (1) The crack propagation directions in the models were affected by the maximum principal stress and hole spacing. When the borehole diameter was fixed, the fracturing pressure increased with increasing hole spacing. In contrast, when the fracturing pressure was fixed, the fracturing range increased with increasing borehole diameter; (2) The most ideal fracturing effect was found at a fracturing pressure of 17.6 MPa in the model with a borehole diameter of 40 mm and hole spacing of 400 mm. The results showed that it is possible to regulate the falls of hard roofs using the SFM. This research may provide a theoretical basis for controlling hard roofs in mining.
Current Research on Antiepileptic Compounds
Authors: Wei Cheng-Xi , Bian Ming , Gong Guo-Hua
Published: November 2015

Epilepsy affects about 1% of the world’s population. Due to the fact all antiepileptic drugs (AEDs) have some undesirable side effects and about 30% of epileptic patients are not seizure-free with the existing AEDs, there is still an urgent need for the development of more effective and safer AEDs. Based on our research work on antiepileptic compounds and other references in recent years, this review covers the reported work on antiepileptic compounds which are classified according to their structures. This review summarized 244 significant anticonvulsant compounds which are classified by functional groups according to the animal model data, although there are some limitations in the data. This review highlights the properties of new compounds endowed with promising antiepileptic properties, which may be proven to be more effective and selective, and possibly free of unwanted side effects. The reviewed compounds represent an interesting possibility to overcome refractory seizures and to reduce the percentage of patients with a poor response to drug therapy.
Condensation of Diacetyl with Alkyl Amines: Synthesis and Reactivity of p-Iminobenzoquinones and p-Diiminobenzoquinones
Authors: Espinoza-Hicks Carlos , Bautista Rafael , Frias-Puente Saúl , Pelayo Vanessa , Martínez-Mora Eder , Delgado Francisco , Tamariz Joaquín
Published: November 2015

Condensation reactions between diacetyl and α-branched primary alkylamines under mild and neutral conditions provided a mixture of 2,5-dimethylbenzoquinone(alkylimines), 2,5-dimethylbenzoquinone(bis-alkyldiimines), and N,N′-dialkyl-2,5-dimethylbenzene-1,4-diamines, which were efficiently separated as pure products by column chromatography. Both 2,5-dimethylbenzoquinone(alkylimines) and 2,5-dimethylbenzoquinone(bis-alkyldiimines) underwent an interchange of the alkylimino group when treated with anilines, followed by reductive aromatization, to provide diarylamines and 1,4-dianilinobenzenes, respectively. Evaluation was also made of the reactivity and selectivity of these compounds in the presence of anilines, thiophenols and alkylhalides.
Immobilization of Metal Hexacyanoferrate Ion-Exchangers for the Synthesis of Metal Ion Sorbents—A Mini-Review
Authors: Vincent Thierry , Vincent Chloë , Guibal Eric
Published: November 2015

Metal hexacyanoferrates are very efficient sorbents for the recovery of alkali and base metal ions (including radionuclides such as Cs). Generally produced by the direct reaction of metal salts with potassium hexacyanoferrate (the precursors), they are characterized by ion-exchange and structural properties that make then particularly selective for Cs(I), Rb(I) and Tl(I) recovery (based on their hydrated ionic radius consistent with the size of the ion-exchanger cage), though they can bind also base metals. The major drawback of these materials is associated to their nanometer or micrometer size that makes them difficult to recover in large-size continuous systems. For this reason many techniques have been designed for immobilizing these ion-exchangers in suitable matrices that can be organic (mainly polymers and biopolymers) or inorganic (mineral supports), carbon-based matrices. This immobilization may proceed by in situ synthesis or by entrapment/encapsulation. This mini-review reports some examples of hybrid materials synthesized for the immobilization of metal hexacyanoferrate, the different conditionings of these composite materials and, briefly, the parameters to take into account for their optimal design and facilitated use.
Ursolic Acid—A Pentacyclic Triterpenoid with a Wide Spectrum of Pharmacological Activities
Authors: Woźniak Łukasz , Skąpska Sylwia , Marszałek Krystian
Published: November 2015

Ursolic acid (UA) is a natural terpene compound exhibiting many pharmaceutical properties. In this review the current state of knowledge about the health-promoting properties of this widespread, biologically active compound, as well as information about its occurrence and biosynthesis are presented. Particular attention has been paid to the application of ursolic acid as an anti-cancer agent; it is worth noticing that clinical tests suggesting the possibility of practical use of UA have already been conducted. Amongst other pharmacological properties of UA one can mention protective effect on lungs, kidneys, liver and brain, anti-inflammatory properties, anabolic effects on skeletal muscles and the ability to suppress bone density loss leading to osteoporosis. Ursolic acid also exhibits anti-microbial features against numerous strains of bacteria, HIV and HCV viruses and Plasmodium protozoa causing malaria.
Two New Cyototoxic Cardenolides from the Whole Plants of Adonis multiflora Nishikawa & Koki Ito
Authors: Jung Jae-Woo , Baek Nam-In , Hwang-Bo Jeon , Lee Seung-Su , Park Ji-Hae , Seo Kyeong-Hwa , Kwon Jung-Hwa , Oh Eun-Ji , Lee Dae-Young , Chung In-Sik , Bang Myun-Ho
Published: November 2015

A phytochemical investigation of the whole plants of Adonis multiflora Nishikawa & Koki Ito. resulted in the isolation and identification of two new cardenolides—adonioside A (1) and adonioside B (6)—as well as four known cardenolides: tupichinolide (2) oleandrine (3), cryptostigmin II (4), and cymarin (5). Their structures were elucidated on the basis of NMR, MS, and IR spectroscopic analyses. Compounds 1, 2, 5, and 6 showed significant cytotoxicity against six human cancer cell lines (HCT-116, HepG2, HeLa, SK-OV-3, and SK-MEL-5, and SK-BR-3).
Solvatochromic and Single Crystal Studies of Two Neutral Triarylmethane Dyes with a Quinone Methide Structure
Authors: Chulvi Katherine , Costero Ana , Ochando Luis , Gil Salvador , Vivancos José-Luis , Gaviña Pablo
Published: November 2015

The crystal structure of two neutral triarylmethane dyes with a p-quinone methide core was determined by X-ray diffraction analysis. The spectroscopic characteristics of both compounds in 23 solvents with different polarities or hydrogen-bonding donor (HBD) abilities has been studied as a function of three solvatochromic parameters (ET(30), π* and α). Both compounds 1 and 2 showed a pronounced bathochromic shift of the main absorption band on increasing solvent polarity and HBD ability. The correlation is better for compound 2 than for compound 1. The stronger effect and better correlation was observed for compound 2 with the increment of the solvent HBD ability (α parameter).
An Efficient and Recyclable Nanoparticle-Supported Cobalt Catalyst for Quinoxaline Synthesis
Authors: Rajabi Fatemeh , Alves Diego , Luque Rafael
Published: November 2015

The syntheses of quinoxalines derived from 1,2-diamine and 1,2-dicarbonyl compounds under mild reaction conditions was carried out using a nanoparticle-supported cobalt catalyst. The supported nanocatalyst exhibited excellent activity and stability and it could be reused for at least ten times without any loss of activity. No cobalt contamination could be detected in the products by AAS measurements, pointing to the excellent activity and stability of the Co nanomaterial.
Synthesis, Crystal Structure, Absolute Configuration and Antitumor Activity of the Enantiomers of 5-Bromo-2-chloro-N-(1-phenylethyl)pyridine-3-sulfonamide
Authors: Zhou Zhixu , Li Linwei , Yan Ning , Du Lei , Sun Changshan , Sun Tiemin
Published: November 2015

Pyridinesulfonamide is an important fragment which has a wide range of applications in novel drugs. R- and S-isomers of 5-bromo-2-chloro-N-(1-phenylethyl)pyridine-3-sulfonamide have been synthesized, and the stereostructures have been researched. Single crystals of both compounds were obtained for X-ray analysis, and the absolute configurations (ACs) have been further confirmed by electronic circular dichroism (ECD), optical rotation (OR) and quantum chemical calculations. The crystal structures and calculated geometries were extremely similar, which permitted a comparison of the relative reliabilities of ACs obtained by ECD analyses and theoretical simulation. In addition, the effect of stereochemistry on the PI3Kα kinase and anticancer activity were investigated. Compounds 10a and 10b inhibit the activity of PI3Kα kinase with IC50 values of 1.08 and 2.69 μM, respectively. Furthermore, molecular docking was performed to analyze the binding modes of R- and S-isomers.
Clones of FeSOD, MDHAR, DHAR Genes from White Clover and Gene Expression Analysis of ROS-Scavenging Enzymes during Abiotic Stress and Hormone Treatments
Authors: Zhang Yan , Li Zhou , Peng Yan , Wang Xiaojuan , Peng Dandan , Li Yaping , He Xiaoshuang , Zhang Xinquan , Ma Xiao , Huang Linkai , Yan Yanhong
Published: November 2015

Increased transcriptional levels of genes encoding antioxidant enzymes play important protective roles in coping with excessive accumulation of reactive oxygen species (ROS) in plants exposed to various abiotic stresses. To fully elucidate different evolutions and functions of ROS-scavenging enzymatic genes, we isolated iron superoxide dismutase (FeSOD), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) from white clover for the first time and subsequently tested dynamic expression profiles of these genes together with previously identified other antioxidant enzyme genes including copper zinc superoxide dismutase (Cu/ZnSOD), manganese superoxide dismutase (MnSOD), glutathione reductase (GR), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) in response to cold, drought, salinity, cadmium stress and exogenous abscisic acid (ABA) or spermidine (Spd) treatment. The cloned fragments of FeSOD, DHAR and MDHAR genes were 630, 471 and 669 bp nucleotide sequences encoding 210, 157 and 223 amino acids, respectively. Phylogenetic analysis indicated that both amino acid and nucleotide sequences of these three genes are highly conservative. In addition, the analysis of genes expression showed the transcription of GR, POD, MDHAR, DHAR and Cu/ZnSOD were rapidly activated with relatively high abundance during cold stress. Differently, CAT, APX, FeSOD, Cu/ZnSOD and MnSOD exhibited more abundant transcripts compared to others under drought stress. Under salt stress, CAT was induced preferentially (3–12 h) compared to GR which was induced later (12–72 h). Cadmium stress mainly up-regulated Cu/ZnSOD, DHAR and MDHAR. Interestingly, most of genes expression induced by ABA or Spd happened prior to various abiotic stresses. The particular expression patterns and different response time of these genes indicated that white clover differentially activates genes encoding antioxidant enzymes to mitigate the damage of ROS during various environmental stresses.
Rice and Bean Targets for Biofortification Combined with High Carotenoid Content Crops Regulate Transcriptional Mechanisms Increasing Iron Bioavailability
Authors: Dias Desirrê , De Castro Moreira Maria , Gomes Mariana , Lopes Toledo Renata , Nutti Marilia , Pinheiro Sant’Ana Helena , Martino Hércia
Published: November 2015

Iron deficiency affects thousands of people worldwide. Biofortification of staple food crops aims to support the reduction of this deficiency. This study evaluates the effect of combinations of common beans and rice, targets for biofortification, with high carotenoid content crops on the iron bioavailability, protein gene expression, and antioxidant effect. Iron bioavailability was measured by the depletion/repletion method. Seven groups were tested (n = 7): Pontal bean (PB); rice + Pontal bean (R + BP); Pontal bean + sweet potato (PB + SP); Pontal bean + pumpkin (PB + P); Pontal bean + rice + sweet potato (PB + R + P); Pontal bean + rice + sweet potato (PB + R + SP); positive control (Ferrous Sulfate). The evaluations included: hemoglobin gain, hemoglobin regeneration efficiency (HRE), gene expression of divalente metal transporter 1 (DMT-1), duodenal citocromo B (DcytB), ferroportin, hephaestin, transferrin and ferritin and total plasma antioxidant capacity (TAC). The test groups, except the PB, showed higher HRE (p < 0.05) than the control. Gene expression of DMT-1, DcytB and ferroportin increased (p < 0.05) in the groups fed with high content carotenoid crops (sweet potato or pumpkin). The PB group presented lower (p < 0.05) TAC than the other groups. The combination of rice and common beans, and those with high carotenoid content crops increased protein gene expression, increasing the iron bioavailability and antioxidant capacity.
Nutrient Composition of Retail Samples of Australian Beef Sausages
Authors: Cunningham Judy , Nguyen Van , Adorno Paul , Droulez Veronique
Published: November 2015

Some nutrient data for beef sausages in Australia’s food composition table, NUTTAB 2010, is over 25 years old and may no longer reflect the composition of this popular food. To update this, 41 retail samples of fresh beef sausages were purchased in Melbourne, Australia, in May 2015. Each purchase was analysed, uncooked, for moisture, protein and fat. Sausages were then grouped by fat content into one of three composites and analysed for a wide range of nutrients, before and after dry heat cooking, the most popular sausage cooking method. Fat content in raw sausages averaged 14.9 g/100 g, 30% lower than NUTTAB values, varying from 7.3 to 22.6 g/100 g. This indicates it is possible to formulate leaner sausages that meet consumer expectations and may qualify for certain nutrition labelling statements. Under current Australian labelling requirements, two low fat sausages contain sufficient protein, B12, niacin, phosphorus and zinc to qualify as a good source of these nutrients and sufficient iron, selenium and vitamin A to qualify as a source of these. Sodium levels are higher than fresh beef, ranging from 680 to 840 mg/100 g. These data will be used to update NUTTAB and support product labelling and consumer education.
Full Genomic Characterization of a Saffold Virus Isolated in Peru
Authors: Leguia Mariana , Loyola Steev , Rios Jane , Juarez Diana , Guevara Carolina , Silva Maria , Prieto Karla , Wiley Michael , Kasper Matthew , Palacios Gustavo , Bausch Daniel
Published: November 2015

While studying respiratory infections of unknown etiology we detected Saffold virus in an oropharyngeal swab collected from a two-year-old female suffering from diarrhea and respiratory illness. The full viral genome recovered by deep sequencing showed 98% identity to a previously described Saffold strain isolated in Japan. Phylogenetic analysis confirmed the Peruvian Saffold strain belongs to genotype 3 and is most closely related to strains that have circulated in Asia. This is the first documented case report of Saffold virus in Peru and the only complete genomic characterization of a Saffold-3 isolate from the Americas.
Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility
Authors: Diaz Lantada Andres , Alarcón Iniesta Hernán , García-Ruíz Josefa
Published: November 2015

This paper presents the complete development and experimental validation of a microsystem designed to systematically assess the impact of surface topography on cell adhesion and dynamics. The microsystem includes two pools for culturing cells and for including chemicals. These pools are connected by several channels that have different microtextures, along which the cells crawl from one well to another. The impact of channel surface topography on cell performance, as well as the influence of other relevant factors, can therefore be assessed. The microsystem stands out for its being able to precisely define the surface topographies from the design stage and also has the advantage of including the different textures under study in a single device. Validation has been carried out by culturing human mesenchymal stem cells (hMSCs) on the microsystem pre-treated with a coating of hMSC conditioned medium (CM) produced by these cells. The impact of surface topography on cell adhesion, motility, and velocity has been quantified, and the relevance of using a coating of hMSC-CM for these kinds of studies has been analyzed. Main results, current challenges, and future proposals based on the use of the proposed microsystem as an experimental resource for studying cell mechanobiology are also presented.
Epirubicin-Complexed Polypeptide Micelle Effectively and Safely Treats Hepatocellular Carcinoma
Authors: Zhang Qinglin , Ding Jianxun , Lv Chenwei , Xu Weiguo , Sun Xun , Meng Xiangwei
Published: November 2015

Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related mortality worldwide. Epirubicin (EPI) once acted as a main agent for HCC chemotherapy. However, the dosage-dependent side effects seriously limit its application in clinic. The purpose of this study is to develop an effective nanocarrier to improve the efficacy and overcome the limitations of EPI. In this regard, the EPI-complexed micelle (i.e., mPEG-b-PGA/EPI) was prepared via the electrostatic interaction between the amino group in EPI and the carboxyl group in PGA segment of methoxy poly(ethylene glycol)-block-poly(l-glutamic acid) (mPEG-b-PGA), and the subsequent hydrophobic interaction among PGA/EPI complexes. The micelle appeared spherical with a diameter at around 90 nm and possessed a pH-sensitive release property of payload. The cytotoxicity and hemolysis assays in vitro, and the maximum tolerated dose tests in vivo confirmed that mPEG-b-PGA was a kind of safe material with excellent biocompatibility, while the drug-loaded micelle could obviously improve the tolerance of EPI. In addition, mPEG-b-PGA/EPI possessed significantly enhanced antitumor efficacy and security toward the H22-xenografted HCC murine model at macroscopic and microscopic levels compared with free EPI. All these results strongly indicate that mPEG-b-PGA/EPI may be a promising nanoplatform for EPI delivery in the chemotherapy of HCC.
New Proposal for Flexural Strengthening of Reinforced Concrete Beams Using CFRP T-Shaped Profiles
Authors: Kotynia Renata , Cholostiakow Szymon
Published: November 2015

The purpose of this study was to evaluate the performance of a novel strengthening system using T-shaped carbon fiber reinforced polymer (CFRP) profiles. The proposed system successfully combines the advantages of two established strengthening techniques, namely the near surface mounted (NSM) and externally bonded (EB) methods. The paper presents the experimental results of structural tests carried out on seven flexurally-strengthened and two non-strengthened full-scale reinforced concrete (RC) members. Two T-shaped profiles having heights of 15 and 30 mm were applied. The main parameters of concrete strength and composite strengthening ratio were investigated to evaluate the efficiency of the proposed flexural strengthening system. All specimens were tested under a quasi-static six-point bending configuration. The test results showed a significant increase in the load bearing capacity and the stiffness of the RC beams with strengthening and also a notable reduction in maximum deflections. The high tensile strength utilization of the CFRP profiles places this strengthening technique as a promising alternative to other, less structurally-efficient systems.
The Impact of Guarantees on the Performance of Pension Saving Schemes: Insights from the Literature
Authors: Bohnert Alexander
Published: November 2015

Guarantees are often seen as the key characteristics of pension saving products, but securing them can become costly and is of central relevance especially in the course of the current low interest rate environment. In this article, we deal with the question of how costly the typical types of guarantees are, in the sense that they reduce a pension saving scheme’s financial performance over time. In this context, we aim to provide a presentation of insights from selected literature studying the impact of point-to-point guarantees and cliquet-style interest rate guarantees on the performance of pension contracts. The comparative analysis emphasizes that, in most cases, guarantee costs are not negligible with regard to a contract’s financial performance, especially compared to benchmarks, and that customers knowingly opt for such guarantees (or not) is, thus, indispensable. To further investigate the willingness-to-pay for guarantees in life insurance is an area for future research, in particular for innovative contract design.
Satellite Image Time Series Decomposition Based on EEMD
Authors: Kong Yun-long , Meng Yu , Li Wei , Yue An-zhi , Yuan Yuan
Published: November 2015

Satellite Image Time Series (SITS) have recently been of great interest due to the emerging remote sensing capabilities for Earth observation. Trend and seasonal components are two crucial elements of SITS. In this paper, a novel framework of SITS decomposition based on Ensemble Empirical Mode Decomposition (EEMD) is proposed. EEMD is achieved by sifting an ensemble of adaptive orthogonal components called Intrinsic Mode Functions (IMFs). EEMD is noise-assisted and overcomes the drawback of mode mixing in conventional Empirical Mode Decomposition (EMD). Inspired by these advantages, the aim of this work is to employ EEMD to decompose SITS into IMFs and to choose relevant IMFs for the separation of seasonal and trend components. In a series of simulations, IMFs extracted by EEMD achieved a clear representation with physical meaning. The experimental results of 16-day compositions of Moderate Resolution Imaging Spectroradiometer (MODIS), Normalized Difference Vegetation Index (NDVI), and Global Environment Monitoring Index (GEMI) time series with disturbance illustrated the effectiveness and stability of the proposed approach to monitoring tasks, such as applications for the detection of abrupt changes.
Automatic Detection and Segmentation of Columns in As-Built Buildings from Point Clouds
Authors: Díaz-Vilariño Lucía , Conde Borja , Lagüela Susana , Lorenzo Henrique
Published: November 2015

Over the past few years, there has been an increasing need for tools that automate the processing of as-built 3D laser scanner data. Given that a fast and active dimensional analysis of constructive components is essential for construction monitoring, this paper is particularly focused on the detection and segmentation of columns in building interiors from incomplete point clouds acquired with a Terrestrial Laser Scanner. The methodology addresses two types of columns: round cross-section and rectangular cross-section. Considering columns as vertical elements, the global strategy for segmentation involves the rasterization of a point cloud onto the XY plane and the implementation of a model-driven approach based on the Hough Transform. The methodology is tested in two real case studies, and experiments are carried out under different levels of data completeness. The results show the robustness of the methodology to the presence of clutter and partial occlusion, typical in building indoors, even though false positives can be obtained if other elements with the same shape and size as columns are present in the raster.