bluuebunny/crossref_metadata_2025 huggingface dataset:
Mengukur Implementasi Distribusi Barang Berdasarkan Kerangka Islamdi Bidang Peternakan dan Konfeksi Pakaian Muslim Kota Tasikmalaya – Jawa Barat
Authors: Nasution Fatimah Zahra
Published: June 2020

Islam memandang urgensi penerapan nilai-nilai halal tidak hanya pada aspek materi, tetapi juga pada aspek penerapan cara hidup (muamalah). Dalam perekonomian, kegiatan distribusi menjadi salah satu kegiatan penting selain konsumsi dan produksi. Bisnis berbasis Islam tidak akan sempurna tanpa kegiatan distribusi yang sesuai dengan prinsip-prinsip Islam. Nilai monoteisme (persatuan), rasa syukur, keadilan dan kebebasan menjadi dimensi dalam mengukur prinsip variabel distribusi Islam yang dianalisis dalam penelitian ini. Penelitian ini bertujuan untuk mengetahui penerapan prinsip Islam dalam kegiatan distribusi yang dilakukan oleh produsen makanan dan non-pangan di Kota Tasikmalaya, Jawa Barat. Data dikumpulkan dengan menggunakan instrumen penelitian dalam bentuk kuesioner yang dibagikan kepada 51 responden yang terdiri dari pengusaha di bidang peternakan yang mewakili produsen makanan, sedangkan dari 88 responden yang terdiri dari pengusaha penganan pakaian muslim yang mewakili produsen bukan makanan. Hasil penelitian menunjukkan bahwa pemahaman pengusaha konpeksi pakaian ternak dan muslim terhadap prinsip distribusi Islam adalah 99,22% dan 98,11%, masing-masing. Dimensi yang paling dominan diterapkan dari prinsip distribusi Islam, baik oleh pengusaha ternak dan pengusaha konpeksi pakaian muslim adalah dimensi monoteisme (kesatuan) dengan nilai rata-rata per item masing-masing 233,0 dan 416,56. Implikasi dari hasil penelitian ini mendorong pengusaha Muslim untuk menerapkan prinsip distribusi Islam dalam kegiatan bisnis mereka untuk mencapai manfaat masyarakat.
EVALUATION OF SULFURIC ACID, BARIUM CHLORIDE, AND SEED GERMINATION ASSAY METHODS AS EARLY PREGNANCY DETECTION INSTRUMENTS IN CATTLE
Authors: Azmi Zul , Desem Muhammad I. , Desem Muhammad I. , S. Purba Hastuti Handayani , S. Purba Hastuti Handayani , Endrawati Dwi , Endrawati Dwi , Rachmawati Faidah , Rachmawati Faidah , Kusumaningtyas Eni , Kusumaningtyas Eni , Subekti Didik T. , Subekti Didik T.
Published: June 2020

This study aims to validate and re-evaluate the use of H2SO4, BaCl2, and seed germination assay (SGA) methods to detect early pregnancy in cattle. Validation was carried out in order to find out the actual test performance value, which include accuracy, sensitivity, and specificity.Validation test was done by reacting the urine of pregnant and non-pregnant cattle populations with H2SO4 solution diluted at a ratio of 1 : 4, with 1% BaCl2 solution or through germination of mung bean seeds in Petri dishes. The results of this study indicated that the H2SO4 and BaCl2 methods have poor accuracy (21.88% and 28.13%) with the sensitivity of 81.82% and 100% and specificity of 9.43% and 13.21%, respectively. Meanwhile, the SGA method had a fairly good accuracy (74.55%) with sensitivity and specificity of 45.46% and 81.82%, respectively. Based on these results, the H2SO4, BaCl2, and SGA methods are not recommended for detection of early pregnancy in the field.
KOSAKONIA SP. PROTEOLYTIC BACTERIA ISOLATED FROM RUMEN AND RETICULUM OF ACEH CATTLE
Authors: Safika Safika , Sari Wenny Novita , Ademi Gressha Vionalle , Arsih Ulfi Widi , Darmawi Darmawi
Published: June 2020

The aim of this study was to identify proteolytic bacteria from the ruminal and reticulum fluids of aceh cattle based on the 16S rRNA gene. Samples used were ruminal and reticulum fluids of aceh cattle slaughtered in Abattoir of Aceh Besar. Samples were diluted and cultured into Skim Milk Agar medium at 39 C for 48 hours. The morphology of bacterial colonies growth in the medium was observed. Colonies resulted in the largest clear zone were isolated and used for Deoxyribonucleic Acid (DNA) isolation, 16S rRNA gene amplification and sequencing. Theresults showed that morphology of dominant colonies was yellowish white color, round shape, position on the agar surface. The results ofphylogenetic analysis of RS1 and ReS2 isolates isolated from rumen and reticulum fluids of aceh cattle respectively had a close familialrelationship and belonged to the bacterial group of Kosakonia. Sequence homology showed isolate RS1 and ReS2 are probably either newEnterobacteriaceae species or unconfirmed species. Halo zone produced by ruminal bacteria had a wider diameter (25 mm vs 20 mm) than thatcaused by reticulum bacteria). Based on the results, RS1 (bacterium in the rumen) and ReS2 (bacterium in the reticulum) belong to similar type, namely Kosakonia sp. with a proteolytic activity. Presumably, these bacteria originate from the rumen that enters the reticulum with degraded feed.
FEEDING LOCAL FERMENTED WITH EM-4, AMMONIUM SULFATE, AND UREA ON THE BLOOD FAT LEVEL OF MALE DUCK
Authors: Septinova Dian , Arti Aisyah Yuli , Sutrisna Rudy , Tantalo Syahrio , Hartono Madi
Published: June 2020

The objective of this study was to determine the effect of commercial ration by the addition of ammonium sulfate and urea fermented feed ingredients on the blood fat levels of local male ducks. This study used a completely randomized design with 32 male ducks which weredivided into four groups namely R0, R1, R2, R3, and R4. Each group was given the following treatment: R0 only given commercial ration;R1 was given 80% commercial ration + 20% fermentation of EM-4 + 1.5% urea); R2 was given 80% commercial ration + 20% EM-4 + 1.5%ammonium sulfate fermentation; and R3 was given 80% commercial ration + 20% EM-4 + 1.5% urea + 1.5% ammonium sulfate. Variablesin this study were blood fat consisting of cholesterol, triglyceride, High-Density Lipoprotein (HDL), and Low-Density Lipoproteins (LDL).The data obtained were analyzed by using 5% real level and LSD. The results showed that the provision of commercial ration added withlocal feed fermented with ammonium sulfate and urea had a significant effect on cholesterol levels (P0.5), but not significant (P0.05) ontriglyceride, HDL, and LDL levels. Furthermore, the ration that gave the best effect on the blood fat levels of male ducks blood was the ration given to R3 group.
Chemosensory Dysfunction, Oral Disorders and Oral Health-Related Quality Of Life in Patients with Primary Sjögren's Syndrome: Comparative Cross-Sectional Study
Authors: Gobeljić Mirjana Šijan , Milić Vera , Pejnovic Nada , Damjanov Nemanja
Published: June 2020

Background: The aim of this study was to evaluate chemosensory function and oral disorders in patients with primary Sjögren's syndrome (pSS) and to compare these findings with those of age- and gender-matched healthy controls.Methods: This comparative cross-sectional study included 58 patients with primary Sjögren’s syndrome (pSS) and 55 age- and gender-matched healthy controls. Olfactory and gustatory function, burning sensations in the tongue (BST) and halitosis were assessed. Oral health-related quality of life (OHRQoL) was evaluated using the short-form Oral Health Impact Profile (OHIP-14).Results: Patients with pSS had significantly lower self-reported visual analogue scale (VAS) smell score (8.6±2.2 vs. 9.6±0.7, p=0.016) and VAS taste score (9.5±0.7 vs. 8.5±2.1, p=0.014) than healthy controls. A greater proportion of patients with pSS had anosmia (3.8% vs. 0.0%) or hyposmia (36.5% vs. 13.2%) and ageusia for basic tastes: sweetness (34.0% vs. 7.5%), sourness (10.6 % vs. 0.0), saltiness (10.0% vs. 5.7%) or bitterness (19.1% vs. 1.9%) as evaluated using Sniffin Sticks test and taste stripts, respectively. A higher proportion of pSS patients complained of dysgeusia (52.6% vs. 9.4%, p<0.0001) and BST (45.6% vs. 0.0%, p<0.0001), while similar number of patients with pSS and controls reported halitosis (31.6% vs. 28.3%, p=0.434). The mean OHIP-14 score was significantly higher in patients with pSS (6.8±7.0 vs. 2.3±8.5, p<0.001) indicating patients’ poorer OHRQoL compared with controls. Conclusions: The majority of patients with pSS had impaired chemosensory function and indicators of oral health in comparison with the age- and gender-matched healthy controls. Further studies of oral hygiene habits and dietary intake of these patients are needed to ensure better management of oral health problems in patients with pSS.
Effects of Low Back Pain on Motor Performance in Older People: A Systematic Review and Meta-analysis
Authors: Ge Le , Wang Chuhuai , Zhou Haohan , Yu Qiuhua , Li Xin
Published: June 2020

Background: Research suggests that individuals with low back pain (LBP) may have poorer motor control compared to their healthy counterparts; however, the sample population of almost 90% of related articles are young and middle-aged people. There is still a lack of a systematic review about the motor performance of elderly people with low back pain. This study aimed to conduct a systematic review and meta-analysis to understand the effects of LBP on motor performance in eldly people.Methods: This systematic review and meta-analysis included a comprehensive search of PubMed, Embase, and Cochrane Library databases for full-text articles published before January 2020. Two independent reviewers screened the relevant articles, and disagreements were resolved by athird reviewer.Results: Thirteen case-control studies comparing motor performance parameters between LBP and healthy subjects and four randomised controlled studies on physical therapy to improve the motor performance of elderly people with LBP were included. The experimental group (LBP group) was associated with significantly larger area of centre of pressure movement (P < 0.001),higher velocity of centre of pressure sway in the anteroposterior and mediolateral directions (P= 0.01 and P = 0.02, respectively), longer path length in the anteroposterior direction (P < 0.001), slower walking speed (P = 0.05), and longer timed up and go test time (P = 0.004) thanthe control group.Conclusion: The results show that motor performance is impaired in elderly people with LBP. We should pay more attention to the balance control of elderly people with LBP.
A Mixed-methods Evaluation of a Person-centered Family Planning Intervention for Community Health Workers in India on Family Planning Outcomes
Authors: Diamond-Smith Nadia , McDonell Claire , Sahu Ananta Basudev , Roy Kali Prasad , Giessler Katie
Published: June 2020

Background: Person-centered quality for family planning has been gaining increased attention, yet few interventions have focused on this,or measured associations between PCFP and family planning outcomes (uptake, continuation, etc.). In India, the first point of contact for family planning is often the community health care worker, in this case, Accredited Social Health Activists (ASHAs). Methods: In this study, we evaluate a training on person-centered family planning as an add-on to a training on family planning provision for urban ASHAs in Varanasi, India in 2019 using mixed methods. We first validate a scale to measure person-centered family planningin a community health worker population, and find it to be valid. Higher person-centered family planningscores are associated with family planning uptake. Results: Comparing women who saw intervention compared to control ASHAs, we find that the intervention had no impact on overall person-centered family planningscores. Women in the intervention arm were more likely to report that their ASHA had a strong preference about what method they choose, suggesting that the training increased provider pressure. However, qualitative interviews with ASHAs suggest that they value person-centered care for their interactions and absorbed the messages from the intervention.Conclusions: More research is needed on how to intervene to change behaviors related to person-centered family planning.This study received IRB approval from the University of California, San Francisco (IRB # 15-25950) and was retrospectively registered at clinicaltrials.gov (NCT04206527) on 20 December 2019.https://clinicaltrials.gov/ct2/show/NCT04206527?term=NCT04206527&draw=2&rank=1
Comparison of different detection methods for Mycoplasma pneumoniae infection in children with community-acquired pneumonia
Authors: Tang Mingyu , Wang Dong , Tong Xing , Zhang Jing , Zhang Lei , Wu Yufen , Cao Qing , Yin Yong
Published: June 2020

Background: Due to the lack of a sensitive, specific and rapid detection method, etiological diagnosis of pneumonia caused by Mycoplasma pneumoniae (M. pneumoniae, MP) is a constantly challenging issue. This retrospective study aimed to compare the diagnostic methods for mycoplasma pneumoniae in children and evaluate their values.Methods: From November 2018 to June 2019, 830 children with community-acquired pneumonia were selected from the Department of Respiratory Medicine, Shanghai Children's Medical Center. On the first day of hospitalization, sputum, throat swabs, venous blood samples were collected for MP-IgM (particle agglutination, PA), MP-IgM (immune colloidal gold technique, GICT), MP-DNA, MP-RNA (simultaneous amplification and testing, SAT), and MP-DNA (real-time polymerase chain reaction, RT-PCR)Results: Among these 830 children, RT-PCR showed that the positive rate was 36.6% (304/830), in which the positive rate of macrolide-resistant (A2063G mutation) accounted for 86.2% (262/304). Using RT-PCR as the standard, MP-RNA (SAT) had the highest specificity (97.5%), and MP-IgM (PA) had the highest sensitivity (74.0%) and Youden index (53.7%). If MP-RNA (SAT) was combined with MP-IgM (PA), its Kappa value (0.602), sensitivity (84.2%), specificity (78.7%), Youden index (62.9%), were higher than those of single Mycoplasma pneumoniae detection.Conclusions: Our research indicated that a combination of MP-RNA (SAT) plus MP-IgM (PA) as an early diagnostic method for children with clinical manifestations of Mycoplasma pneumoniae pneumonia might lead to reliable results.
Research title on Multilevel Modelling of Time to Death for Under-Five Children: 2016 Ethiopia Demographic and Health Survey, 2019
Authors: Argawu Alemayehu Siffir , Hirko Gudeta
Published: June 2020

Background: Reducing child mortality is now a global concern. Globally, Under-five child mortality rate was decreased by 58% in 2017. In the 2016 EDHS report, under-five mortality was declined to 60% in Ethiopia in 2016. Methods: The data for the study was obtained from EDHS data conducted in 2016. In the study, we analysed the data using stratified Cox proportional hazard model and multilevel lognormal parametric survival model. Results: From the total of 10,331 under-five children, 635 (6.1%) deaths had occurred in the 2016 EDHS data. And, the overall probability of survival value was near to 0.92 with the estimated mean survival time was 55.4 months. In the study we found that covariates like birth type of the child, family size, wealth index, frequency of listening radio, place of delivery, place of residence, and geographical region were significant factors for the death of under-five children in stratified Cox proportional hazard model. In the multilevel lognormal parametric survival model, we found that the random-intercept effects of variations between region and household levels on the mean survival times of the children were 1.7 and 0.9, respectively. These values indicated that we had enough evidence for the existence of unobserved heterogeneities between regions and households. Conclusion: The covariates like birth type of the child, family size, wealth index, frequency of listening radio, place of delivery, place of residence, and geographical region covariates were significant factors for under-five children mortality using stratified Cox proportional hazard regression model. In the random-intercept effects model, the two estimated variances of the random-intercept effects for regional and household levels were 1.7 and 0.9, respectively. The values indicate that we have enough evidence that there were unobserved heterogeneities on the mean survival times of the under-five children between regions and households levels. Further studies should be conducted to identify the individual, household, and community-level factors associated with infant and child mortality in Ethiopia.
Developing an Online Infectious Disease Outbreak Database: Codifying the World Health Organization’s Disease Outbreak News reports
Authors: Katz Rebecca , Carlson Colin J , Pavez Munir C , Lin Jessica , Palys Max A , Boyce Matthew R
Published: June 2020

Background: The World Health Organization’s Disease Outbreak News (DONs) reports are the world’s primary source of official information on global disease outbreaks. Access to this information is crucial for informing research analyses, global health priorities, and decision making. However, in its current form, the utility of the DONs reports for research and analysis is limited as a result of their reporting format. To this end, we designed a standardized methodology for codifying the data contained in DONs reports and created an online, searchable database. Methods: We coded DONs reports published between the years 1996 and 2019, systematically collecting data from each individual report using a standardized methodology and tabulating data into a single spreadsheet. We created a Year-Pathogen-Country taxonomy to group related disease events and circumvent issues related to reporting inconsistencies in DONs reports.Results: In total, we reviewed 2,806 DONs reports corresponding to 1,105 unique infectious disease outbreaks from 1996-2019. Overall, H5N1 represented the most frequently reported disease, while China was the country with the most reports. We observed the DONs reports to contain numerous issues relating to the standardization, accuracy, and transparency of reporting procedures.Conclusions: Our database represents a new, accessible resource for research that improves the accessibility of the data contained in DONs reports. The World Health Organization should consider standardizing reporting practices, protocols, and procedures as a means of improving the reporting and transparency of infectious disease outbreaks.Trial Registration: N/A
Do Physical Activity-Related Knowledge, Attitudes, and Behaviors Play a Role in the Elderly's Mental Health? A Cross-Sectional Study from the China Health and Nutrition Survey
Authors: Yang Ying , Wei Liuyi , He Di , Hu Borui , Li Qianqiu , Mao Zongfu
Published: June 2020

Background: Physical activity (PA)-related knowledge, attitudes, and behaviors (KABs) are important content of health management. We conducted this study to (a) describe PA-related KABs of Chinese older adults, and (b) examine the association between PA-related KABs and mental health.Methods: This study used data from the 2015 China Health and Nutrition Survey (CHNS). 4048 residents aged 60 years and older were included as study subjects, assessing their PA-related knowledge, attitudes, behaviors, and mental health. Comparison of PA-related KABs in urban and rural areas, as well as in different mental health groups were conducted using chi-square test. Ordinal logistic regression analysis was applied to assess the associations between PA-related KABs and mental health.Results: The proportion for knowing about the benefits of physical activity, knowing about appropriate exercise intensity, and knowing about healthy weight were 76.7%, 44.3%, and 75.4%, respectively. 89.2% of the participants hold positive attitude towards physical activity. 45.0%, 12.1%, and 12.9% of the participants reported preferring walking & Tai Chi, ball games, and body building, respectively. Participants in urban areas had significantly better PA-related KABs than those in rural areas (all p-values < 0.001). Adjusted ORs with 95% CI showed that participants who knew about the benefits of physical activity (OR = 1.19, 95% CI = 1.02-1.38); hold positive attitude towards physical activity (OR = 1.31, 95% CI = 1.08-1.59); and reported preferring walking & Tai Chi (OR = 1.40, 95% CI = 1.23-1.60), ball games (OR = 1.45, 95% CI = 1.17-1.79), body building (OR = 1.33, 95% CI = 1.09-1.63) had significant better mental health.Conclusions: Elderly residents in rural areas experienced significant poorer PA-related KABs than those in urban areas, especially for PA-related behaviors. Better PA-related knowledge, attitudes, and behaviors were associated with higher mental health in Chinese elderly residents.
Early Embryo Development in Women with Endometriosis: A Retrospective Cohort Study
Authors: Hamdan Mukhri B. , Cotterill M , Price A , Cheong Y
Published: June 2020

Background: Endometriosis is known to be detrimental to fertility in many ways. Evidence drawn from studies on oocyte recipient cycles suggests that the oocyte quality of women with endometriosis is compromised. Research that specifically examined the association between embryo quality and endometriosis/endometrioma are scarce and the question if endometriosis affects oocyte and/or embryo quality remain controversial. This study aims to evaluate embryo quality of women with endometriosis (EN) and without endometriosis (tubal factor [TF], unexplained infertility [UE]).Methods: Multi-centre retrospective study was performed. Treatment cycles and embryology records of women with and without endometriosis undergoing IVF was reviewed via IDEASTM database. Number of oocytes collected per mature follicle, fertilisation rate, pregnancy rate, morphology and morphometric assessment of embryo at all stages were evaluatedResults: Total of 678 women who had IVF treatment was analysed (EN, n=89; TF, n=214; UE, n=375). The mean age for each group was EN, 34.4±3.3; TF, 33.5±3.7 and 34.6±3.2 respectively. Number of mature follicles (>14mm) on the day of trigger injection was similar (P=0.75) between all groups. Percentage of oocytes collected per mature follicle was lower in endometriosis compared to other groups (P=0.01; EN, 65±23; TF, 76±20; UE, 71±24). Higher percentages of embryos fail to achieve 8-cell stage in EN compared to control groups (P=0.02; EN, 4.0±1.6 TF, 1.2±0.4; UE, 1.5±0.4. Percentages of embryos at all grades (Grade1-4) per women were similar between the comparison groups (P>0.05). Endometriosis did not impair (P>0.05) blastocyst development or the development of Fully Expanded Hatching Blastocysts (FEHB).Conclusions: This study finds women with endometriosis have lower number of oocyte per total number of follicle. The presence of endometriosis is associated with a higher rate of early embryo arrest, which implicates poor oocyte quality. However endometriosis has no effect on the embryo quality beyond the 8-cell-stage of embryo development
The predictive value of low urine specific gravity in the diagnosis of vasovagal syncope in children and adolescents
Authors: Liu Ping , Zeng Xingfang , Yang Hong , Mei Wanzhen , Wang Yuwen , Zhang Juan , Zou Runmei , Wang Cheng
Published: June 2020

Background
Vasovagal syncope (VVS) is a kind of common neurogenic syncope in children and adolescents. Decreased blood volume is one of the pathogenesis of VVS. The study will explore the predictive value of low urine specific gravity (USG) in the diagnosis of VVS in children and adolescents.
Methods
97 cases, 43 males and 54 females, aged 4 to 16 years old, with an average age of 10.91 ± 2.18 years old, due to unexplained premonitory syncope or syncope were selected. They were diagnosed with VVS through head-up tilt table test. During the same period, 91 cases of children and adolescents, including 45 males and 46 females, aged from 5 to 15 years old, who underwent a healthy examination were matched as a control (control group). USG was measured in both groups.
Results
The USG of VVS group was significantly lower than that of the control group (P < 0.01). The sensitivity and specificity of USG prediction in the diagnosis of VVS were evaluated by ROC curve. The area under the ROC curve was 0.751, standard error was 0.035, and 95% CI (0.683, 0.819) suggested that USG was of moderate predictive value in the diagnosis of VVS. Setting USG as < 1.0185, the sensitivity and specificity and diagnostic coincidence rate of VVS were 74.39%, 66.04% and 69.68%, respectively.
Conclusion
Low USG has predictive value in the diagnosis of VVS in children and adolescents.
Biochar addition alleviate the negative effects of drought and salinity stress on soybean productivity and water use efficiency
Authors: Zhang Yaojun , Ding Jiaqi , Wang Hong , Su Lei , Zhao Cancan
Published: June 2020

Background: Environmental stress is a crucial factor restricting plant growth as well as crop productivity, thus influencing the agricultural sustainability. Biochar addition is proposed as an effective management to improve crop performance. However, there were few studies focused on the effect of biochar addition on crop growth and productivity under interactive effect of abiotic stress (e.g., drought and salinity). This study was conducted with a pot experiment to investigate the interaction effects of drought and salinity stress on soybean yield, leaf gaseous exchange and water use efficiency (WUE) under biochar addition. Results: Drought and salinity stress significantly depressed soybean phenology (e.g. flowering time) and all the leaf gas exchange parameters, but had inconsistent effects on soybean root growth and WUE at leaf and yield levels. Salinity stress significantly decreased photosynthetic rate, stomatal conductance, intercellular CO2 concentration and transpiration rate by 20.7%, 26.3%, 10.5% and 27.2%, respectively. Lower biomass production and grain yield were probably due to the restrained photosynthesis under drought and salinity stress. Biochar addition significantly enhanced soybean grain yield by 3.1-14.8%. Drought stress and biochar addition significantly increased WUE-yield by 27.5% and 15.6%, respectively, while salinity stress significantly decreased WUE-yield by 24.2%. Drought and salinity stress showed some negative interactions on soybean productivity and leaf gaseous exchange. But biochar addition alleviate the negative effects on soybean productivity and water use efficiency under drought and salinity stress. Conclusions: The results of the present study indicated that drought and salinity stress could significantly depress soybean growth and productivity. There exist interactive effects of drought and salinity stress on soybean productivity and water use efficiency, while we could employ biochar to alleviate the negative effects. We should consider the interactive effects of different abiotic restriction factors on crop growth thus for sustainable agriculture in the future.
Zinc As A Factor Affecting Serum Calcification Propensity in Patients With Type 2 Diabetes Mellitus
Authors: Nakatani Shinya , Mori Katsuhito , Sonoda Mika , Nishide Kozo , Uedono Hideki , Tsuda Akihiro , Emoto Masanori , Shoji Testuo
Published: June 2020

Background: Zinc inhibits vascular calcification in vivo and in vitro. Patients with type 2 diabetes mellitus show hypozincemia and are at an elevated risk of cardiovascular events. Recently, the in vitro test (T50-test) was developed for the determination of serum calcification propensity. This cross-sectional study investigated the association between serum zinc and T50 in type 2 diabetes mellitus patients and the effect of zinc on T50in vitro.Methods: The subjects were 132 type 2 diabetes mellitus patients with various kidney function. We measured T50 levels by the established nephelometric method.Results: The median (interquartile range) levels of T50 and serum zinc were 306 (269 to 332) min, and 80.0 (70.1 to 89.8) µg/dL, respectively. Serum zinc level was significantly and positively correlated with T50 (rs = 0.219, p = 0.012). This association remained significant in multivariable-adjusted analysis, and was independent of known factors including phosphate, calcium, and magnesium. Renal function and glycemic control were not significantly associated with T50. Finally, addition of physiological concentration of exogenous zinc chloride significantly increased the serum T50 in vitro.Conclusions: This is the first report to investigate the association between serum calcification propensity and zinc levels in type 2 diabetes mellitus patients. Our data suggest that serum zinc is an independent factor that inhibits serum calcification propensity.
LincHOXA10 Facilitates Colorectal Cancer Development By Regulating HOXA10-Mediated Epithelial-Mesenchymal Transtion
Authors: Zhu Huifang , Li Yongzhen , Zhang Yinghui , Zhang Zheying , Wang Yongxia , He Guoyang , Li Na , Wang Haijun , Zhong Jiateng , Qian Xinlai , Hu Yuhan
Published: June 2020

Background: Long non-coding RNAs (lncRNAs) have been reported to play an important role in tumorigenesis and metastasis of human colorectal cancer (CRC). However, the specific role of LincHOXA10 in CRC remains unknown.Methods: The expression of LincHOXA10 and HOXA10 in CRC cells and tissue samples was measured by quantitative reverse transcription PCR (qRT-PCR). The protein expression of HOXA10, E-cadherin, N-cadherin, Vinmentin, p-smad2 and p-smad3 was assessed by Western blotting or immunofluorescence staining. Cell proliferation, migration, and invasion were assessed by the MTT and transwell assays. Tumor growth in vivo was carried out by subcutaneous tumor formation in nude mice.Results: In the present study, we found that LincHOXA10 expression was significantly higher in human CRC tissues than the paired normal tissues. In fact, LincHOXA10 level correlated with the CRC tumor sizes and lymphatic metastasis. In cultured CRC cells, knockdown of LincHOXA10 inhibited cell proliferation, migration and invasion. LincHOXA10 deficiency also attenuated CRC tumor growth in vivo. Mechanistically, LincHOXA10 interacted with HOXA10 and regulated its expression. HOXA10 levels were interrelated to the LincHOXA10 level in CRC cells. Functionally, HOXA10 was essential for TGF-β1/SMADs-induced epithelial -mesenchymal transition of CRC cells, and HOXA10 played a critical role in mediating the function of LincHOXA10. Importantly, HOXA10 expression was significantly up-regulated in human CRC tissues.Conclusions: LincHOXA10 facilitates CRC development and metastasis via regulating HOXA10-mediated epithelial-mesenchymal transition of CRC cells.
Pulsed Shortwave Therapy in Cervical Osteoarthritis: An NSAID- Controlled, Randomized Clinical Trial
Authors: Mohameed Rachid El , Koneru Sree , Staelin Richard , McLeod Kenneth , Tabbouche Omar , Rawe Ian
Published: June 2020

Objective Assess treatment superiority of Pulsed Shortwave Therapy (PSWT) against COX-2 NSAID therapy, in reducing disability and pain due to cervical osteoarthritis.Design 200chronic pain suffers (average pain duration about 2 years)diagnosed with cervical osteoarthritis by radiological imaging were randomized into one of two treatmentarms: COX-2 NSAID treatment: Etoricoxib 60mg/day for 4 weeks; or, PSWT treatmentworn 24 hours/day for 4 weeks. The primary outcome measure was the 4-week score on the Neck Disability Index (NDI): a 10-question assessment on a 50-point scale. Secondary outcome measures included pain(at rest and during activity)measured on a Visual Analog Scale (VAS)of0-100 mm, dose count of rescue pain medication (paracetamol)use and a treatment satisfaction rating. These 4-week scores were compared across the two arms to assess superiority.Results After 4 weeks of treatment,subjects in both study arms reported significantly lower (p<0.0001) 4-week measures (11.24-NSAID;9.34-PSWT; 0-50 points),VASrest (30.08-NSAID;22.76-PSWT; 0-100 mm) and VASactivity (36.40-NSAID; 27.42-PSWT; 0-100 mm).The absolute reduction from baseline in NDI was significantly greater in the PSWT arm than NSAID arm(by 3.66 points; 95% CI 2.3 to 5.02; p<0.0001). Similarly, the reductions from baseline inVASrest and VASactivitywere significantly greater in the PSWT arm than NSAID arm(by 10.89 mm;95% CI 6.90 to 14.87; p<0.0001 and 12.05 mm;95% CI 7.76 to 16.33; p<0.0001 respectively).The PSWT arm used 50% less rescue pain medication. Eleven adverse effects were reported in the NSAID arm and zero in the PSWT arm.Conclusion Both NSAID and PSWT treatments resulted in clinically meaningful increases in quality of life(NDI) and decreases in pain (VAS) associated with cervicalosteoarthritis. However, the PSWT armshowed superior improvements in all outcome measures when compared to the NSAID armwith no adverse effects.
Mapping the Multidimensionality of Medical Care related Catastrophe on Households: A Study of Four Blocks in Birbhum District, West Bengal
Authors: Dasgupta Priyanka , Mukherjee Subrata
Published: June 2020

Background

Medical care related catastrophe is generally identified by healthcare expenses crossing a certain percentage of household’s resources (Wagstaff and van Doorslaer, 2003). This paper attempts to examine medical care related ‘catastrophe’ by going beyond the threshold-oriented approach of catastrophic medical expenditure and include multiple indicators which seeks to explore the catastrophe from a multidimensional perspective.
Methods

Drawing from multidimensional vulnerability to poverty approach (Alkire and Forster, 2008), we provide a measure which incorporates multiple indicators that might put households in medical care related catastrophic situation. Our study uses data from a cross-sectional household survey conducted by the Society for Health and Demographic Surveillance (SHDS) of the Government of West Bengal in 2012. Using negative binomial and logistic regression, the study also attempts to find the correlates of healthcare utilization, incurring catastrophic health expenditure for both 10 per cent of household consumption expenditure and 40 per cent of non-food expenditure as well as resorting to distress financing and availing low quality/ no care despite chronic illness.
Results

Estimates show that illness, presence of elderly members, hospitalization and outpatient visits increases the risk of incurring catastrophic health expenditure and healthcare utilization counts. In addition, households belonging to backward socio-religious categories and having members engaged as casual labourers face higher odds of distressed financing and availing informal/ no healthcare. In contrast to Wagstaff and van Doorslaer’s measure of catastrophic medical expenses, the multidimensional measure shows a lower medical care catastrophe for the upper economic classes and forward caste groups and vice versa which is more realistic and convincing.
Conclusions

The evidence generated from the multidimensional analysis presents a more convincing and reliable picture of vulnerability imparted due to health shocks as compared to identifying households with catastrophic medical expenditure by Wagstaff and van Doorslaer’s method. However, this study has its limitations as it has given equal weightage to all the dimensions and restricted itself to headcount measures. However, extension and refinement of this approach can provide more insightful findings.
Is Coracoclavicular Reconstruction Necessary in Hook Plate Fixation for Acute Unstable Acromioclavicular Dislocation?
Authors: Chen Yu-Ta , Wu Kuan-Ting , Jhan Shun-Wun , Hsu Shan-Lin , Liu Hao-Chen , Wang Ching-Jen , Ko Jih-Yang , Chou Wen-Yi
Published: June 2020

Background: Acromioclavicular joint (ACJ) dislocation is a relatively common shoulder injury. For the treatment of cases of severe ACJ dislocation (Rockwood type III–V), hook plate fixation is an easy-to-master and minimally-invasive approach to surgical intervention. Overstress on the acromion following hook plate fixation often leads to acromial complications such as osteolysis and loss of reduction. We hypothesized that suspensory reconstruction alongside hook plate fixation might provide a superior stability and reduce complications as compared with hook plate fixation alone. The purpose of the study was to assess the clinical and radiographic outcomes of these two surgical modalities.Methods: We retrospectively enrolled 38 patients with acute ACJ dislocation from May 2010 to December 2018. Among them, nineteen patients received hook plate fixation only (HP group), and 19 underwent concomitant hook plate fixation and loop suspension fixation with two mersilene tapes (HM group). The demographic data of the patients were recorded and analyzed. All patients underwent a shoulder x-ray initially, immediately postoperatively, and at 1, 3, 6 and 12 months to measure the relative coracoclavicular distance (rCCD). Clinical assessment of shoulder function outcome was conducted using the Constant Murley Score (CMS); the University of California at Los Angeles (UCLA) Shoulder Score was also measured at the latest follow-up.Results: There were no significant differences in the demographic data between the two groups. With regards to the CMS and the UCLA score, the HM group and HP group both had excellent outcomes, and no significant differences in scores were observed between groups (CMS: 93.90 ± 6.16 versus 94.47 ± 7.26, p = 0.47; UCLA score: 32.84 ± 2.91 versus 34.32 ± 1.16, p = 0.07). However, the HM group demonstrated substantial superiority in terms of maintenance of the rCCD over the HP group (91.47 ± 27.47 versus 100.75 ± 48.70, p = 0.015). In addition, there was less subacromial osteolysis in the HM group than the HP group (52.6% versus 15.8%, p = 0.038).Conclusion: Concomitant hook plate fixation with loop suspensory reconstruction yielded excellent functional outcomes and superior maintenance of the rCCD with fewer acromion complications than hook plate fixation alone.
Superior Articular Process Osteotomy Navigation (SapON) Technology in Percutaneous Endoscopic Lumbar Discectomy: A New Surgical Technology
Authors: Li Jianhui , Ma Boyuan , Wang Mengyao , Li Jianwei , Tian Shuijing , Sun Chuan , Shi Guidong , Zhou Xianhu
Published: June 2020

Background: This research aims to discuss the clinical application value of the superior articular process osteotomy navigation (SapON) technique in percutaneous endoscopic lumbar discectomy.Methods: Patients who underwent intervertebral foraminal endoscopic surgery for degenerative disease of the lumbar spine were collected and divided into two groups from March 2016 to May 2018. Group I: 69 patients were treated by the SapON technique. Group II: 53 patients were treated by Transforaminal Endoscopic Spine Surgery (TESS) technique. Fluoroscopy times, the time of establishment working channel, endoscopic operations, and entire operations in two groups were recorded. Patients' clinical curative effect was evaluated by visual analog scale (VAS) scores, Oswestry disability index(ODI), and MacNab standard at different time points, including before surgeries, one day, three months after surgeries, and the last follow-up time.Results: Operations were all successful both in groups I and II. Fluoroscopy times, establishing the working tunnels time, endoscopic operations time, and entire operations time and other indexes between the two groups were statistically significant. Patients’ low back, legs pain VAS scores, and ODI one day, three months after surgeries, and at the last follow-up in both two groups were significantly lower than those before operations with a statistical difference. The excellent rate in groups I and II were 98.55% and 96.23%. Complications were not observed in group I, while in group II, only one patient suffered the injury of the right L5 nerve root, and recovered after three months’ conservative treatment.Conclusion: The application of SapON in percutaneous endoscopic lumbar discectomy (PELD) has its advantages to popularize in clinical work, including operating simply and orderly, high safety, fewer complications, lower radiation exposure, and good clinical effects
Pseudogene KRT17P3 Drives Cisplatin Resistance of Human NSCLC Cells by Modulating miR-497-5p/mTOR
Authors: Hou Zhibo , Zhou Qianwen , Xia Ning , Lv Tang-feng , Yuan Xiaoqin , Song Yong
Published: June 2020

Background: Chemoresistance is a major obstacle in non-small-cell lung cancer (NSCLC) treatment. The pseudogene KRT17P3 has been shown to be up-regulated in lung cancer tissues from patients with cisplatin resistance. However, its molecular mechanism in promoting chemoresistance has not been elucidated.Methods: Real-time PCR was performed to evaluate KRT17P3 levels in plasma samples collected from 30 cisplatin-resistance patients and 32 cisplatin-sensitive patients. KRT17P3 was overexpressed or knocked down in A549 and SK-MES-1 NSCLC cells to evaluate the effects of KRT17P3 on the in vitro and in vivo resistance of NSCLC to cisplatin. Cell Counting Kit 8, Annexin V/propidium iodide staining, and PARP-1 cleavage assays were performed to evaluate cell viability and apoptosis. Dual luciferase reporter, RNA immunoprecipitation, Western blot assays, and rescue experiments were used to evaluate the functional interaction of KRT17P3, miR-497-5p and mTOR.Results: Our results demonstrate that KRT17P3 overexpression in cultured NSCLC cells increases cell viability and decreases apoptosis upon cisplatin treatment, while KRT17P3 knockdown has the opposite effect. KRT17P3 overexpression promotes NSCLC tumor growth upon in cisplatin-treated xenografted mice. Mechanistically, KRT17P3 acts as a molecular sponge for miR-497-5p and relieves the binding of miR-497-5p to its target gene mTOR. Rescue experiments validated the functional interaction among KRT17P3, miR497-5p, and mTOR. Moreover, the plasma level of KRT17P3 is up-regulated in cisplatin-resistance patients.Conclusions: Our findings indicate that KRT17P3/miR497-5p/mTOR regulates the chemosensitivity of NSCLC, suggesting a potential therapeutic target for cisplatin-resistant NSCLC patients. KRT17P3 may be a potential peripheral blood marker of NSCLC patients resistant to cisplatin.
Analysis of Biliary Microbiota in Hepatobiliopancreatic Diseases Compared to Healthy People [MICROBILIO]: Study Protocol
Authors: Nascimento Fernanda Sayuri do , Suzuki Milena Oliveira , Taba João Victor , de Mattos Vitoria Carneiro , Pipek Leonardo Zumerkorn , D’Albuquerque Eugênia Machado Carneiro , Meyer Alberto , Andraus Wellington , Pinho João Renato Rebello , de Moura Eduardo Guimarães Hourneaux , Setubal João Carlos , Carneiro-D’Albuquerque Luiz Augusto
Published: June 2020

Background: The performance of the microbiota is observed in several digestive tract diseases. Therefore, reaching the biliary microbiota may suggest ways for studies of biomarkers, diagnoses, tests and therapies in hepatobiliopancreatic diseases.Methods: Bile samples will be collected in endoscopic retrograde cholangiopancreatography patients (case group) and living liver transplantation donors (control group). From the DNA extracted from the samples, the microbiomes will be sequenced by 16S ribosomal RNA (rRNA) pyrosequencing methods. Student’s t-test will be performed with SPSS version 20 for Windows and the Mann-Whitney test will be performed using R software and Python scripts. A principal component analysis signals will be developed as discriminative analysis.Results: The primary expected results of the study is to characterize the specific composition of the biliary microbiota in situations of disease and health. In addition, it seeks to demonstrate the existence of changes in the case of illness and also possible disease biomarkers, diagnosis, interventions and therapies in hepatobiliopancreatic diseases.Conclusions: We believe that the investigation of the biliary tract microbiota will provide benefits such as early diagnosis and new treatments. This should improve quality of life and survival, specially in malignant diseases. Thus, this research will give expectations to further investigation in the biliary microbiota.Trial registration: NCT04391426. Registered 18 May 2020, https://clinicaltrials.gov/ct2/show/NCT04391426
GM-CSF Expression and Macrophage Polarization in Joints of Undifferentiated Arthritis Patients Evolving to Rheumatoid Arthritis or Psoriatic Arthritis
Authors: Fuentelsaz-Romero Sara , Cuervo Andrea , Estrada-Capetillo Lizbeth , Celis Raquel , García-Campos Raquel , Ramírez Julio , Sastre Sergi , Samaniego Rafael , Puig-Kröger Amaya , Cañete Juan D
Published: June 2020

Background and Aims: GM-CSF-dependent macrophage polarization hasbeen demonstrated in rheumatoid arthritis (RA). Our aim was to seek diagnostic/prognostic biomarkers for undifferentiated arthritis (UA) by analyzing GM-CSF expression and source, macrophage polarization and density in joints of patients with UA evolving to RA or PsA compared with established RA or PsA, respectively.Methods:Synovial tissue (ST)from patients with UA evolving to RA (UA>RA, n=8), PsA (UA>PsA, n=9), persistent UA (UA, n=16), established RA (n=12) and PsA (n=10), and healthy controls (n=6), were analyzed. Cell source and quantitative expression of GM-CSF and proteins associated with pro-inflammatory (GM-CSF-driven) and anti-inflammatory (M-CSF-driven) macrophage polarization (activin A, TNFα, MMP12 and CD209, respectively) were assessed in ST CD163+ macrophages by multicolor immunofluorescence. GM-CSF and activin A levels were also quantified in paired synovial fluid samples. CD163+ macrophage density was determined in all groups by immunofluorescence.Results:Synovial stromal cells (FAP+ fibroblast, CD90+ endothelial cells) and CD163+ sublining macrophages were the sources of GM-CSF. ST CD163+ macrophages from all groups expressed pro-inflammatory polarization markers (activin A, TNFα and MMP12). Expression of the M-CSF-dependent anti-inflammatory marker CD209 identified two macrophage subsets (CD163+ CD209high and CD163+ CD209low/-). CD209+ macrophages were more abundant in ST from healthy controls and PsA patients, although both macrophage subtypesshowed similar levels of pro-inflammatory markers in all groups. In paired synovial fluid samples, activin A was detected in all patients, with higher levels in UA>RA and RA, while GM-CSF was infrequently detected. ST CD163+ macrophage density was comparable between UA>RA and UA>PsA patients, but significantly higher than in persistent UA and established RA and PsA patients, respectively.Conclusions: GM-CSF is highly expressed by sublining CD90+ FAP+ synovial fibroblasts, CD90+ activated endothelium and CD163+ macrophages in different types of arthritis. The polarization state of ST macrophages was similar in all UA and established arthritis groups, with a predominance of pro-inflammatory GM-CSF-associated markers. CD163+ macrophage density was significantly higher in the UA phases of RA and PsA compared with persistent UA. Taken together, our findings support the idea that GM-CSF is a strong driver of macrophage polarization and a potential therapeutic target not only in RA but also in PsA and all types of UA.