bluuebunny/crossref_metadata_2025 huggingface dataset:
Taste and smell disturbances in patients with chronic oral graft vs. host disease: An observational study
Authors: Boor Marlou , Raber-Durlacher Judith E. , Hazenberg Mette D. , Rozema Frederik R. , Laheij Alexa M. G. A.
Published: September 2022

Background

A common complication of allogeneic hematopoietic stem cell transplantation (alloHSCT) is chronic oral graft vs. host disease (cGvHD). Oral cGvHD may present as mucosal lesions, salivary gland dysfunction, and trismus. Moreover, taste and smell ability may be affected, but the prevalence, nature and severity of altered taste and smell function, and their impact on quality of life (QoL) are understudied.

Aim

To identify the prevalence, nature, and severity of taste and smell disturbances, their impact on QoL and to assess whether altered taste/smell ability is associated with oral mucosal cGvHD or hyposalivation.

Materials and methods

AlloHSCT recipients at least 100 days post-HSCT and referred for oral cGvHD-related oral complaints were eligible for participation in this cross-sectional study. Manifestations of oral mucosal cGvHD were scored, the (un)stimulated salivary flow was measured, and objective taste and smell ability was evaluated. Subjective taste and smell alterations, and overall and oral health (OH)-related QoL were assessed.

Results

In total, 45 patients were included, of which objective reduced taste ability (hypogeusia) was identified in 68.9%; 28.9% had reduced smell ability and 11.1% had complete loss of smell. Nevertheless, only 31.1% of patients reported severe taste alterations and 22% reported moderate taste alterations indicating that not all the patients were aware of their altered taste sense. Taste/smell disturbances were not related to oral mucosal cGvHD or hyposalivation. Most alloHSCT recipients reported a decreased OH-related QoL. However, a relation between taste/smell ability and global or OH-related QoL could not be identified.

Conclusion

Taste and smell disturbances are prevalent among alloHSCT recipients. Most patients reported a decreased OH-related QoL, but the specific impact of taste and smell disturbances remains to be elucidated.
The climate and ocean risk vulnerability index: Measuring coastal city resilience to inform action
Authors: Rouleau Tracy , Stuart Jack , Call Maia , Yozell Sally , Yoshioka Nagisa , Maekawa Miko , Fiertz Natalie
Published: September 2022

Today, coastal cities worldwide are struggling with the complex interaction of environmental threats, economic development, and societal inequity. The acceleration of global climate change, which will impact ocean health, sea level, rainfall patterns, and temperatures, will only further exacerbate the ongoing challenges faced by coastal cities. Coastal cities face interconnected risks that necessitate the use of a data collection and an assessment approach that can assess these impacts through a holistic lens. Risk is the interaction of hazards, exposure, and vulnerability, and while data on hazards and exposure is becoming more widely available, data on the vulnerability of urban coastal populations remains limited. These data gaps are particularly acute for the Global South, where climate change is expected to have the greatest near-term impacts. Policymakers need city-specific data to best understand their levels of risk and engage in effective adaptation planning. This paper introduces the Climate and Ocean Risk Vulnerability Index (CORVI), its conceptual framework, methodology, and protocol. The article also demonstrates the application of CORVI through two pilot projects in Castries, Saint Lucia and Kingston, Jamaica. It concludes with a reflection of lessons learned from the pilot projects, and an assessment of the utility of the structured expert judgement (SEJ) for collecting data and measuring risk in data sparse environments. This paper makes two primary contributions. First it introduces CORVI, a decision support tool that assesses climate risk and resilience in a coastal city. The tool uses the SEJ methodology to display risk scores across 10 risk categories and 94 indicators addressing ecological, financial, and political risk. Second, it demonstrates the use of the SEJ methodology in data sparse environments.
Increased risk of water quality deterioration under climate change in Ganga River
Authors: Santy Sneha , Mujumdar Pradeep , Bala Govindasamy
Published: September 2022

The industrialized stretch of Kanpur is considered to be one of the most polluted stretches of the Ganga River, with untreated sewage, industrial discharge, and agricultural runoff. Risk assessment studies on water quality for future scenarios are limited for this stretch of the river. In this study, we assess the effect of climate change on water quality, the risk of eutrophication, and fish kill for the mid and end of the twenty-first century for this river stretch. The water quality parameters considered are dissolved oxygen (DO), biochemical oxygen demand (BOD), ammonia, nitrate, total nitrogen (TN), organic-, inorganic- and total phosphorous (TP), and fecal coliform (FC). The risk of eutrophication and fish kill are quantified using simulated concentrations of nutrients and DO, respectively. Downscaled climate change projections for two climate change scenarios (RCP4.5 and RCP8.5) are used to drive a hydrological model coupled to a water quality simulation model. Our simulations indicate a potential deterioration of water quality in this stretch in the mid-twenty-first century, with a potential increase in pollutant concentration by more than 50% due to climate change alone. However, a slight improvement is simulated by the end of the century relative to the mid-twenty-first century which can be attributed to increased streamflow during low-flow periods due to increased summer mean precipitation. The risk of reduced dissolved oxygen and increased organic and nutrient pollution, and the risk of eutrophication and fish kill increase with warming due to the rise in the frequency of low-flow events and a reduction in streamflow during low-flow events. However, the risk of nitrate and microbial pollution is reduced because of an increased denitrification rate and pathogen decay rate with warming. The risk of eutrophication and fish kill is found to increase by 43.5 and 15% due to climate change alone by mid-twenty-first century. Our findings could be helpful to planners in water resource management to take necessary actions to improve the water quality of the Ganga River in this century.
Generalized uncertainty principle and burning stars
Authors: Moradpour H. , Ziaie A. H. , Sadeghnezhad N. , Ghasemi A.
Published: September 2022

Gamow’s theory of the implications of quantum tunneling for star burning has two cornerstones: quantum mechanics and the equipartition theorem. It has been proposed that both of these foundations are affected by the existence of a non-zero minimum length, which usually appears in quantum gravity scenarios and leads to the generalized uncertainty principle (GUP). Mathematically, in the framework of quantum mechanics, the effects of the GUP are considered as perturbation terms. Here, generalizing the de Broglie wavelength relation in the presence of a minimal length, GUP corrections to the Gamow temperature are calculated, and in parallel, an upper bound for the GUP parameter is estimated.
Influence of crowd size on home advantage in the Japanese football league
Authors: Nomura Ryota
Published: September 2022

This study aimed to investigate the influence of crowd size on home advantage (HA). Data of the 2019 and 2020 seasons of the J1 League (Japan Professional Football's First Division League) were analyzed. Matches during the 2019 season were played under regular conditions, while there was low stadium occupancy during the 2020 season to prevent the spread of COVID-19. Regarding average points won, HA disappeared. By using multiple group structural equation modeling, it was examined the routes of influence via which a reduction in crowd size influenced HA. The results indicated that the influence from the crowd size to the referee's decisions disappeared during the 2020 season. However, the factors including the referee's decisions have lower effects on the outcome factor. Hence, no dominant route was detected in the present study.
Uncommon abdominal “egg-shelled” lesions mimic hepatic echinococcosis: Two cases report
Authors: Liao Yubo , Zhou Guo , Yang Chong , Zhang Yu
Published: September 2022

Background

Primary abdominal “egg-shelled” lesions with positive anti-echinococcus IgG antibody were misdiagnosed as echinococcosis.

Case presentation

Herein we report two cases with primary abdominal “egg-shelled” lesions were misdiagnosed as echinococcosis. Case 1: A 44-year-old woman presented to our department with a history of slight abdominal pain for 4 months. After admission, the laboratory tests indicated a positive anti-echinococcus IgG antibody status. The contrast-enhanced CT scan showed a 12 × 12 cm “me contrast-mass located in the hepatorenal area. The patient had the entire mass and the right adrenal gland resected. This patient recovered smoothly and was discharged uneventfully 20 days after the operation. The pathologic diagnoses was adrenal lymphangioma. Case 2: A 55-year-old woman was admitted with a history of an abdominal mass for over 10 years. After admission, her anti-echinococcus IgG antibody was positive. The contrast-enhanced CT scan revealed a heterogeneous, solid mass measuring 10 × 9 × 8 cm in the right hepatic lobe. A laparoscopic exploration was performed, and the surgery revealed that the mass arose from the retroperitoneal tissue rather than the liver. Finally, the pathologic diagnoses were paraganglioma with necrosis and cystic changes.

Conclusion

Enhanced CT scan and contrast-enhanced ultrasound scans are important for distinguishing echinococcosis disease from the other “egg-shelled” lesions. Surgical resection is the main treatment method for this disease. Minimally invasive surgery is recommended but should be performed by experienced surgical teams. Immunohistochemical examination is important for the pathologic diagnosis.
Traction-bed-assisted reduction and double-plate fixation for treatment of comminuted femoral intertrochanteric fractures with coronal split
Authors: Hu Liangcong , Xie Xudong , Wang Tiantian , Mi Bobin , Xue Hang , Lin Ze , Xiong Yuan , Hu Yiqiang , Zhou Wu , Cao Faqi , Liu Guohui
Published: September 2022

Background

A coronal comminuted femoral intertrochanteric fracture is a special type of fracture that easily leads to internal fixation failure, and the current internal fixation techniques remain controversial. This study aims to evaluate the effect of traction-bed-assisted reduction and double-plate internal fixation in the treatment of comminuted and coronally split intertrochanteric femoral fracture.

Method

Retrospective analyses of the clinical data of 83 patients diagnosed with, and treated for, comminuted and coronally split intertrochanteric femoral fracture from December 2017 to November 2019 were conducted. Among the total number of 83 patients, 40 patients received traction-bed-assisted reduction and PFNA fixation (the control group), whereas 43 patients received traction-bed-assisted reduction and double-plate internal fixation (the experimental group). The major indicators for the research analysis such as the general information of patients, perioperative data, and follow-up data of both groups were collected, sorted out, and meticulously analyzed.

Results

The time taken for traction-bed-assisted reduction and double-plate intern fixation in the experimental group was significantly shorter than that in the control group (P < .05). The post-operative Harris Hip Score (HHS) at 3 months and at the final follow-up after the surgery was significantly better in the experimental group compared with that in the control group, both of which were statistically significant (P < .05). However, there were statistically no significant differences between the two groups in terms of preoperative hemoglobin (Hb) level, amount of intraoperative total blood loss, immediate post-operative Hb level, incidence of wound infection within 14 days post-operatively, time taken to step up on the ground after surgery, HHS 2 weeks after surgery, time taken for fracture healing, and the incidence of complications (P > .05).

Conclusion

The use of a traction bed to achieve adequate reduction, followed by internal fixation using double plates, comparatively takes less time for both reduction and operation in the treatment of comminuted and coronally split intertrochanteric femoral fractures, which also restores proper hip joint movements relatively early and hence provides better hip joint functions in the long run.
Performance of bat-derived macrophages at different temperatures
Authors: Nemcova Monika , Seidlova Veronika , Zukal Jan , Dundarova Heliana , Zukalova Katerina , Pikula Jiri
Published: September 2022

Heterothermy, as a temperature-dependent physiological continuum, may affect host-pathogen interactions through modulation of immune responses. Here, we evaluated proliferation and functional performance of a macrophage cell line established from the greater mouse-eared (Myotis myotis) bat at 8, 17.5, and 37°C to simulate body temperatures during hibernation, daily torpor and euthermia. Macrophages were also frozen to −20°C and then examined for their ability to proliferate in the immediate post-thaw period. We show that bat macrophages can proliferate at lower temperatures, though their growth rate is significantly slower than at 37°C. The cells differed in their shape, size and ability to attach to the plate surface at both lower temperatures, being spheroidal and free in suspension at 8°C and epithelial-like, spindle-shaped and/or spheroidal at 17.5°C. While phagocytosis at temperatures of 8 and 17.5°C amounted to 85.8 and 83.1% of the activity observed at 37°C, respectively, full phagocytic activity was restored within minutes of translocation into a higher temperature. Bat-derived macrophages were also able to withstand temperatures of −20°C in a cryoprotectant-free cultivation medium and, in the immediate post-thaw period, became viable and were able to proliferate. Our in vitro data enhance understanding of macrophage biology.
A Novel High-Speed Third-Order Sliding Mode Observer for Fault-Tolerant Control Problem of Robot Manipulators
Authors: Nguyen Van-Cuong , Tran Xuan-Toa , Kang Hee-Jun
Published: September 2022

In this paper, a novel fault-tolerant control tactic for robot manipulator systems using only position measurements is proposed. The proposed algorithm is constructed based on a combination of a nonsingular fast terminal sliding mode control (NFTSMC) and a novel high-speed third-order sliding mode observer (TOSMO). In the first step, the high-speed TOSMO is proposed for the first time to approximate both the system velocity and the lumped unknown input with a faster convergence time compared to the TOSMO. The faster convergence speed is obtained thanks to the linear characteristic of the added elements. In the second step, the NFTSMC is designed based on a nonsingular fast terminal sliding (NFTS) surface and the information obtained from the proposed high-speed TOSMO. Thanks to the combination, the proposed controller–observer tactic provides excellent features, such as a fast convergence time, high tracking precision, chattering phenomenon reduction, robustness against the effects of the lumped unknown input and velocity requirement elimination. Especially, the proposed observer does not only improve the convergence speed of the estimated signals, but also increases the system dynamic response. The system’s finite-time stability is proved using the Lyapunov theory. Finally, to validate the efficiency of the proposed strategy, simulations on a PUMA560 robot manipulator are performed.
Numerical Analysis of Energy Recovery of Hybrid Loader Actuators Based on Parameters Optimization
Authors: Mu Hongyun , Luo Yanlei , Luo Yu , Chen Lunjun
Published: September 2022

The conventional loader actuator hydraulic system suffers from the potential energy waste problem of the boom arm. This study proposes a hydraulic control method and control strategy for the energy recovery and regeneration of a hybrid loader arm. When the boom arm drops, the piston side of the boom cylinder charges the accumulator, and the system achieves energy recovery. When the boom arm rises, the accumulator releases hydraulic energy to drive the energy regeneration hydraulic motor to provide energy for the system, and the system achieves energy regeneration. The system’s principle analysis and the mathematical model are completed based on Boyle’s, Newton’s second law, and the flow continuity principle. The simulation model is established using AMESim 2D mechanical library, HCD library, and signal library. Under the typical working condition, 50-type wheel loader numerical simulation research is conducted, and the system cylinder motion characteristics, accumulator charging and discharging performance, system energy recovery, and regeneration performance are obtained. On this basis, energy recovery and regeneration efficiency are selected as optimization objectives. The optimal designs of accumulator and energy regeneration hydraulic motor parameters are carried out to obtain the influence law of accumulator and hydraulic motor parameters on system energy recovery and regeneration, and the energy-saving effect of the system is analyzed. The results show that the optimized parameters effectively improve the system energy recovery and regeneration efficiency and reduce engine fuel consumption. The system provides a reference for designing an energy recovery system and researching the energy-saving technology of loaders.
Effect of Sodium Selenite Concentration and Culture Time on Extracellular and Intracellular Metabolite Profiles of Epichloë sp. Isolated from Festuca sinensis in Liquid Culture
Authors: Zhou Lianyu , Xie Huichun , Ma Xuelan , Ju Jiasheng , Luo Qiaoyu , Qiao Feng
Published: September 2022

Selenium (Se) is not only an essential trace element critical for the proper functioning of an organism, but it is also an abiotic stressor that affects an organism’s growth and metabolite profile. In this study, Epichloë sp. from Festuca sinensis was exposed to increasing concentrations of Na2SeO3 (0, 0.1, and 0.2 mmol/L) in a liquid media for eight weeks. The mycelia and fermentation broth of Epichloë sp. were collected from four to eight weeks of cultivation. The mycelial biomass decreased in response to increased Se concentrations, and biomass accumulation peaked at week five. Using gas chromatography-mass spectrometry (GC-MS), approximately 157 and 197 metabolites were determined in the fermentation broth and mycelia, respectively. Diverse changes in extracellular and intracellular metabolites were observed in Epichloë sp. throughout the cultivation period in Se conditions. Some metabolites accumulated in the fermentation broth, while others decreased after different times of Se exposure compared to the control media. However, some metabolites were present at lower concentrations in the mycelia when cultivated with Se. The changes in metabolites under Se conditions were dynamic over the experimental period and were involved in amino acids, carbohydrates, organic acids, fatty acids, and nucleotides. Based on these results, we conclude that selenite concentrations and culture time influence the growth, extracellular and intracellular metabolite profiles of Epichloë sp. from F. sinensis.
Development of a Crop Spectral Reflectance Sensor
Authors: Liu Naisen , Zhang Wenyu , Liu Fuxia , Zhang Meina , Du Chenggong , Sun Chuanliang , Cao Jing , Ji Shuwen , Sun Hui
Published: September 2022

In this study, a low-cost, self-balancing crop spectral reflectance sensor (CSRS) was designed for real-time, nondestructive monitoring of the spectral reflectance and vegetation index of crops such as tomato and rapeseed. The sensor had a field of view of 30°, and a narrow-band filter was used for light splitting. The filter’s full width at half-maximum was 10 nm, and the spectral bands were 710 nm and 870 nm. The sensor was powered by a battery and used WiFi for communication. Its software was based on the Contiki operating system. To make the sensor work in different light intensity conditions, the photoelectric conversion automatic gain circuit had a total of 255 combinations of amplification. The gimbal of the sensor was mainly composed of an inner ring and an outer ring. Under the gravity of the sensor, the central axis of the sensor remained vertical, such that the up-facing and down-facing photosensitive units stayed in the horizontal position. The mechanical components of the sensor were designed symmetrically to facilitate equal mass distribution and to meet the needs of automatic balancing. Based on the optical signal transmission process of the sensor and the dark-current characteristics of the photodetector, a calibration method was theoretically deduced, which improved the accuracy and stability of the sensor under different ambient light intensities. The calibration method is also applicable for the calibration of other crop growth information sensors. Next, the standard reflectance gray scale was taken as the measurement variable to test the accuracy of the sensor, and the results showed that the root mean square error of the reflectance measured by the sensor at 710 nm and 870 nm was 1.10% and 1.27%, respectively; the mean absolute error was 0.95% and 0.89%, respectively; the relative error was below 4% and 3%, respectively; and the coefficient of variation was between 1.0% and 2.5%. The reflectance data measured by the sensor under different ambient light intensities suggested that the absolute error of the sensor was within ±0.5%, and the coefficients of variation at the two spectral bands were 1.04% and 0.39%, respectively. With tomato and rapeseed as the monitoring targets, the proposed CSRS and a commercial spectroradiometer were used to measure at the same time. The results showed that the reflectance measured by the two devices was very close, and there was a linear relationship between the normalized difference vegetation index of the CSRS and that of the commercial spectroradiometer. The coefficient of determination (R2) for tomato and rapeseed were 0.9540 and 0.9110, respectively.
Software-Based Process Simulation and Feasibility Assessment of Black Soldier Fly Larvae Fatty Acid Extraction and Fractionation
Authors: Subakti Felix , Chou Chung-Hsi , Gozan Misri , Lin Yuan-Yu
Published: September 2022

Black soldier flies have been studied as an alternative animal feed. On the other hand, they could be used to yield an abundance of fatty acids. Their omnivorous diet and low space requirements allow for the mass breeding of black soldier flies, using widely available food wastes as feedstock. This study simulates the industrial upscaling of an extraction process for black soldier fly larvae using SuperPro™ 9.5 simulation software. This software contains an extensive material library that regulated physical data for the chemical composition of the larvae and the products. It also bundled several types of bioreactors utilized in bioprocessing. The scheduling of the plant was aided by SchedulePro, which allows for the generation of batch durations and Gantt charts. Four fatty acids were chosen as the main revenue source, with simulated proteins assigned as by-products of the plant. Ash and cellulose were the wastes of the plant, and were separated through multiple filters. The plants were later assessed for their economic feasibility. The kitchen waste plant was the most profitable, and the control variable was the only unprofitable plant. These results may have been impacted by the waste content found in the control variable and the abundance of revenue products in the kitchen-waste-fed larvae.
Diversity of Mitochondrial DNA Haplogroups and Their Association with Bovine Antral Follicle Count
Authors: Liu Hongfei , Zhai Junjun , Wu Hui , Wang Jingyi , Zhang Shaowei , Li Jie , Niu Zhihan , Shen Chenglong , Zhang Kaijuan , Liu Zhengqing , Jiang Fugui , Song Enliang , Sun Xiuzhu , Wang Yongsheng , Lan Xianyong
Published: September 2022

Maternal origins based on the bovine mitochondrial D-loop region are proven to have two main origins: Bos taurus and Bos indicus. To examine the association between the maternal origins of bovine and reproductive traits, the complete mitochondrial D-loop region sequences from 501 Chinese Holstein cows and 94 individuals of other breeds were analyzed. Based on the results obtained from the haplotype analysis, 260 SNPs (single nucleotide polymorphism), 32 indels (insertion/deletion), and 219 haplotypes were identified. Moreover, the nucleotide diversity (π) and haplotype diversity (Hd) were 0.024 ± 0.001 and 0.9794 ± 0.003, respectively, indicating the abundance of genetic resources in Chinese Holstein cows. The results of the median-joining network analysis showed two haplogroups (HG, including HG1 and HG2) that diverged in genetic distance. Furthermore, the two haplogroups were significantly (p < 0.05) correlated with the antral follicle (diameter ≥ 8 mm) count, and HG1 individuals had more antral follicles than HG2 individuals, suggesting that these different genetic variants between HG1 and HG2 correlate with reproductive traits. The construction of a neighbor-joining phylogenetic tree and principal component analysis also revealed two main clades (HG1 and HG2) with different maternal origins: Bos indicus and Bos taurus, respectively. Therefore, HG1 originating from the maternal ancestors of Bos indicus may have a greater reproductive performance, and potential genetic variants discovered may promote the breeding process in the cattle industry.
The Role of Fishing Piers in Brown Pelican (Pelecanus occidentalis) Entanglement
Authors: Thomas Fairl L. , Forys Elizabeth A.
Published: September 2022

Throughout their range, Brown Pelicans (Pelecanus occidentalis) are one of the most common species to become entangled in fishing gear. We surveyed four piers every other week for one year (6/2019–5/2020) in the Tampa Bay region, FL, USA, to determine frequency of pelican entanglement associated with fishing piers, and explored factors that might influence the rate of entanglement. We conducted a generalized linear model (GLM) to determine the influence that pier, pier closure due to COVID-19, time of day and season, number of anglers, and presence of human behaviors that might attract pelicans to the pier had on the number of entangled pelicans. We conducted 144 surveys and counted 3766 pelicans of which 254 (7%) were entangled. The variables significantly associated (p < 0.05) with entanglement were the pier, time of day, and pier closure status, while the number and behavior of anglers were not significant. The two piers that most significantly influenced the number of entanglements both had extensive perches within 10 m of the fishing pier. The management action most likely to reduce the number of entangled pelicans appears to be deterring pelicans from perching near piers or decreasing fishing near perching structures.
Basal Blood Morphology, Serum Biochemistry, and the Liver and Muscle Structure of Weaned Wistar Rats Prenatally Exposed to Fumonisins
Authors: Tomaszewska Ewa , Rudyk Halyna , Wojtysiak Dorota , Donaldson Janine , Muszyński Siemowit , Arciszewski Marcin B. , Lisova Nataliia , Brezvyn Oksana , Puzio Iwona , Abramowicz Beata , Pawłowska-Olszewska Marta , Kotsyumbas Ihor , Dobrowolski Piotr
Published: September 2022

Cereals are often contaminated with fumonisins, which are the toxic byproducts of mold. The aim of the study was to determine the effect of maternal exposure to fumonisins on the development and the liver function of the offspring at weaning. Two doses of fumonisins (60 and 90 mg/kg b.w.) were tested. The changes in the basal blood morphology, the biochemical parameters, the absolute and relative weights of the vital organs, and the changes in the cardiac and biceps brachii muscle histology were studied. The liver damage was assessed by evaluating the liver morphology and the common clinical liver panel. Maternal fumonisin intoxication caused a decrease in the body weight at birth and an increase in the heart, liver, kidney, lungs, ovaries, and testes weights. The cytokines and hormones, as well as the red blood cell counts and hemoglobin levels, were elevated in a dose-dependent manner following the exposure to fumonisins. Maternal exposure caused degenerative morphological and structural changes in the liver, as well as inflammation in the striated muscles, such as the heart and biceps brachii, and disproportionate development of the rat offspring in a dose-dependent manner. Moreover, FB exposure resulted in the disproportional development of the rat offspring in a dose-dependent manner, which was probably caused by the bodily hormonal dysregulation. Prenatal fumonisin exposure can be a pathological precursor for serious diseases, such as obesity and diabetes, later in life.
In Vitro Screening of Non-Antibiotic Components to Mitigate Intestinal Lesions Caused by Brachyspira hyodysenteriae, Lawsonia intracellularis and Salmonella enterica Serovar Typhimurium
Authors: de Groot Nienke , Meneguzzi Mariana , de Souza Barbara , de O. Costa Matheus
Published: September 2022

Swine dysentery, ileitis, and porcine salmonellosis are production-limiting diseases of global importance for swine production. They are caused by infection with Brachyspira hyodysenteriae, Lawsonia intracellularis, and Salmonella enterica serovar Typhimurium, respectively. Currently, the prevention, treatment, and control of these diseases still relies on antimicrobials. The goal of this study was to evaluate the effectiveness of four commercially available non-antimicrobial compounds in preventing lesions caused by the bacteria cited above using an in vitro intestinal culture model. A total of five pigs per pathogen were used and multiple compounds were evaluated. For compound F (a fungal fermented rye), S (a blend of short and medium chain fatty acids), and P (a synergistic blend of short and medium chain fatty acids, including coated butyrates), a total of four explants/pig for each treatment were used, while for compound D (an extract of carob and thyme) only 12 explants/pig for each treatment were used. Explants were exposed to a combination of pathogen only (n = 4/compound/pig), compound only (n = 4/compound/pig), or pathogen and compound (n = 4/compound/pig) and sampled at two time-points. Histopathology and gene expression levels were evaluated to investigate the treatment effect on explants. Short and medium-chain fatty acids, and an extract of carob and thyme, was found to mitigate lesions due to B. hyodysenteriae exposure. A fungal fermented prebiotic increased healthy epithelial coverage when explants were exposed to L. intracellularis or S. Typhimurium. These findings represent a step towards finding alternatives to antimicrobials usage and control of swine dysentery, ileitis, and salmonellosis in pork production.
ESKAPE Pathogens: Looking at Clp ATPases as Potential Drug Targets
Authors: Motiwala Tehrim , Mthethwa Qiniso , Achilonu Ikechukwu , Khoza Thandeka
Published: September 2022

Bacterial antibiotic resistance is rapidly growing globally and poses a severe health threat as the number of multidrug resistant (MDR) and extensively drug-resistant (XDR) bacteria increases. The observed resistance is partially due to natural evolution and to a large extent is attributed to antibiotic misuse and overuse. As the rate of antibiotic resistance increases, it is crucial to develop new drugs to address the emergence of MDR and XDR pathogens. A variety of strategies are employed to address issues pertaining to bacterial antibiotic resistance and these strategies include: (1) the anti-virulence approach, which ultimately targets virulence factors instead of killing the bacterium, (2) employing antimicrobial peptides that target key proteins for bacterial survival and, (3) phage therapy, which uses bacteriophages to treat infectious diseases. In this review, we take a renewed look at a group of ESKAPE pathogens which are known to cause nosocomial infections and are able to escape the bactericidal actions of antibiotics by reducing the efficacy of several known antibiotics. We discuss previously observed escape mechanisms and new possible therapeutic measures to combat these pathogens and further suggest caseinolytic proteins (Clp) as possible therapeutic targets to combat ESKAPE pathogens. These proteins have displayed unmatched significance in bacterial growth, viability and virulence upon chronic infection and under stressful conditions. Furthermore, several studies have showed promising results with targeting Clp proteins in bacterial species, such as Mycobacterium tuberculosis, Staphylococcus aureus and Bacillus subtilis.
Identification of a Novel IncHI1B Plasmid in MDR Klebsiella pneumoniae 200 from Swine in China
Authors: Liang Huixian , Li Xinhui , Yan He
Published: September 2022

Multidrug-resistant (MDR) Klebsiella pneumoniae poses a seriously threat to public health. The aim of this study was to better understand the genetic structure of its plasmids and chromosomes. The whole-genome sequence of K. pneumoniae 200 isolated from the liver of a swine with diarrhea in China was determined using PacBio RS II and Illumina MiSeq sequencing. The complete sequences of the chromosomal DNA and the plasmids were analyzed for the presence of resistance genes. The phylogenetic trees revealed that K. pneumoniae 200 displayed the closest relationship to a human-associated K. pneumoniae strain from Thailand. K. pneumoniae 200 contained two plasmids, pYhe2001 and pYhe2002, belonging to the incompatibility groups IncH-HI1B and IncF-FIA. The plasmid pYhe2001 was a novel plasmid containing four types of heavy metal resistance genes and a novel Tn6897 transposon flanked by two copies of IS26 at both ends. Mixed plasmids could be transferred from K. pneumoniae 200 to Escherichia coli DH5α through transformation together. This study reported the first time a novel plasmid pYhe2001 from swine origin K. pneumoniae 200, suggesting that the plasmids may act as reservoirs for various antimicrobial resistance genes and transport multiple resistance genes in K. pneumoniae of both animal and human origin.
Artificial Intelligence Applied to Medical Imaging and Computational Biology
Authors: Rundo Leonardo , Tangherloni Andrea , Militello Carmelo
Published: September 2022

The Special Issue “Artificial Intelligence Applied to Medical Imaging and Computational Biology” of the Applied Sciences Journal has been curated from February 2021 to May 2022, which covered the state-of-the-art and novel algorithms and applications of Artificial Intelligence methods for biomedical data analysis, ranging from classic Machine Learning to Deep Learning [...]
Influence of Refractive Index Differences on the Signal Strength for Raman-Spectroscopic Measurements of Double Emulsion Droplets
Authors: Hufnagel Thomas , Rädle Matthias , Karbstein Heike P.
Published: September 2022

Double emulsions show great potential for encapsulating active substances and protecting them against external influences. However, they tend to become unstable during storage. Research on double emulsions, therefore, focuses on maintaining their microstructure during their shelf life. Optical measurement methods, such as Raman spectroscopy, have hardly been used to date to analyze the microstructure of double emulsions, mainly due to multiple scattering effects. This study investigates the influence of refractive index matching of double emulsion phases by measuring the Raman signal strength of the inner water phase for different refractive index combinations. Ammonium nitrate and glycerol are added to the inner and outer water phase, respectively, to change the refractive indices of both phases. Additionally, polyvinyl alcohol serves as an emulsifier in the outer water phase. The oil phase consists of silicone oil and Dowsil Resin XR 0497 as the emulsifier. The refractive index of the oil phase is kept constant. For individual phase boundaries of single droplets, the refractive index matching plays a minor role. However, if there are many droplets with correspondingly numerous phase boundaries, which leads to multiple scattering during the measurement, the matching has a significant influence on the signal strength of the inner phase. When measuring double emulsions, the phases should always be matched, as this results in higher signals and improves the sensitivity of the measurement.
A Project Scheduling Game Equilibrium Problem Based on Dynamic Resource Supply
Authors: Feng Cuiying , Hu Shengsheng , Ma Yanfang , Li Zongmin
Published: September 2022

In a resource-constrained project scheduling problem, most studies ignore that resource supply is a separate optimization problem, which is not in line with the actual situation. In this study, the project scheduling problem and the resource supply problem are regarded as a dynamic game system, with interactive influences and constraints. This study proposes a Stackelberg dynamic game model based on the engineering supply chain perspective. In this model, the inherent conflicts and complex interactions between the Multi-mode Resource-Constrained Project Scheduling Problem (MRCPSP) and the Multi-Period Supply Chain Problem (MPSCP) are studied to determine the optimal equilibrium strategy. A two-level multi-objective programming method is used to solve the problem. The MRCPSP is the upper-level planning used to optimize project scheduling and activity mode selection to minimize project cost and duration; MPSCP is a lower-level planning method that seeks to make resource transportation decisions at a lower cost. A two-layer hybrid algorithm, consisting of Genetic Algorithm (GA) and Particle Swarm Optimization (PSO), is proposed to determine the optimal equilibrium strategy. Finally, the applicability and effectiveness of the proposed optimization method are evaluated through a case study of a large hydropower construction project, and management suggestions for related departments are provided.
Bioencapsulation of Microbial Inoculants: Mechanisms, Formulation Types and Application Techniques
Authors: Rojas-Sánchez Blanca , Guzmán-Guzmán Paulina , Morales-Cedeño Luzmaria R. , Orozco-Mosqueda Ma. del Carmen , Saucedo-Martínez Blanca C. , Sánchez-Yáñez Juan M. , Fadiji Ayomide Emmanuel , Babalola Olubukola Oluranti , Glick Bernard R. , Santoyo Gustavo
Published: September 2022

The excessive use of agrochemicals in the field to increase production and counteract the negative effects caused by biotic and abiotic factors has led to a deterioration in soil fertility, plus an increment in negative impacts on the environment and human health. Therefore, the application of beneficial microorganisms as bioinoculants is an eco-friendly alternative to agrochemicals. Plant growth-promoting bacteria and fungi have been effective in promoting plant growth and production, as well as reducing the action of pathogens in multiple crops. However, successful application of such beneficial microorganisms in the agricultural field has faced several difficulties, such as survival, colonization efficiency and short periods of shelf storage. Therefore, it is essential to explore novel ways to encapsulate, formulate and apply bioinoculants. To obtain the expected quality in bioencapsulated products, it is essential to determine the type of polymer, capsule size, encapsulation technique and use the correct chemical and physical cofactors involved in the production process. Thus, this review highlights the various formulation types and application techniques, as well as discussing the multiple advantages of using microbial encapsulates to have better results in agricultural production.
Bacterial Community Analysis of Biofilm Formed on Metal Joint
Authors: Akita Hironaga , Shinto Yoshiki , Kimura Zen-ichiro
Published: September 2022

Microbiologically influenced corrosion (MIC) is caused by biofilms formed on metal surfaces, and MIC of metal alloys on marine infrastructure leads to severe accidents and great economic losses. Although bacterial community analyses of the biofilms collected from corroded metal have been studied, the analyses of biofilms collected from uncorroded metal are rarely reported. In this study, a biofilm formed on an uncorroded metal joint attached to a metal dock mooring at Akitsu Port was used as a model for bacterial community analysis. The bacterial community was analyzed by high-throughput sequencing of the V3–V4 variable regions of the 16S rRNA gene. Bacterial species contained in the biofilms were identified at the genus level, and Alkanindiges bacteria were the dominant species, which have been not reported as the dominant species in previous research on MIC. The genome sequences of known Alkanindiges bacteria do not have conserved gene clusters required to cause metal corrosion, which suggests that Alkanindiges bacteria do not corrode metals but act on the formation of biofilms. Those findings indicated that the bacterial community may change significantly during the process from biofilm formation to the occurrence of metal corrosion.
nl-Selective Classical Charge-Exchange Cross Sections in Be4+ and Ground State Hydrogen Atom Collisions
Authors: Ziaeian Iman , Tőkési Károly
Published: September 2022

Charge-exchange cross sections in Be4+ + H(1s) collisions are calculated using the three-body classical trajectory Monte Carlo method (CTMC) and the quasi-classical trajectory Monte Carlo method of Kirschbaum and Wilets (QCTMC) for impact energies between 10 keV/amu and 300 keV/amu. We present charge-exchange cross sections in the projectile n = 2 and nl = 2s, 2p states. Our results are compared with the previous quantum-mechanical approaches. We found that the QCTMC model is a powerful classical model to describe the state-selective charge-exchange cross sections at lower impact energies and the QCTMC results are in good agreement with previous observations.
Assessing the Role of Ancestral Fragments and Selection Signatures by Whole-Genome Scanning in Dehong Humped Cattle at the China–Myanmar Border
Authors: Luo Xiaoyu , Li Shuang , Liu Yingran , Ahmed Zulfiqar , Wang Fuwen , Liu Jianyong , Zhang Jicai , Chen Ningbo , Lei Chuzhao , Huang Bizhi
Published: September 2022

Dehong humped cattle are precious livestock resources of Yunnan Province, China; they have typical zebu traits. Here, we investigated their genetic characteristics using whole-genome resequencing data of Dehong humped animals (n = 18). When comparing our data with the publicly-available data, we found that Dehong humped cattle have high nucleotide diversity. Based on clustering models in a population structure analysis, Dehong humped cattle had a mutual genome ancestor with Chinese and Indian indicine cattle. While using the RFMix method, it is speculated that the body sizes of Dehong humped cattle were influenced by the Chinese indicine segments and that the immune systems of Dehong humped cattle were affected by additional ancestral segments (Indian indicine). Furthermore, we explored the position selection regions harboring genes in the Dehong humped cattle, which were related to heat tolerance (FILIP1L, ABHD6) and immune responses (GZMM, PRKCZ, STOML2, LRBA, PIK3CD). Notably, missense mutations were detected in the candidate gene ABHD6 (c.870C>A p.Asp290Glu; c.987C>A p.Ser329Arg). The missense mutations may have implications for Dehong humped cattle adaptation to hot environments. This study provides valuable genomic resource data at the genome-wide level and paves the way for future genetic breeding work in the Dehong humped cattle.
Connexins and Pannexins: Important Players in Neurodevelopment, Neurological Diseases, and Potential Therapeutics
Authors: Baracaldo-Santamaría Daniela , Corrales-Hernández María Gabriela , Ortiz-Vergara Maria Camila , Cormane-Alfaro Valeria , Luque-Bernal Ricardo-Miguel , Calderon-Ospina Carlos-Alberto , Cediel-Becerra Juan-Fernando
Published: September 2022

Cell-to-cell communication is essential for proper embryonic development and its dysfunction may lead to disease. Recent research has drawn attention to a new group of molecules called connexins (Cxs) and pannexins (Panxs). Cxs have been described for more than forty years as pivotal regulators of embryogenesis; however, the exact mechanism by which they provide this regulation has not been clearly elucidated. Consequently, Cxs and Panxs have been linked to congenital neurodegenerative diseases such as Charcot-Marie-Tooth disease and, more recently, chronic hemichannel opening has been associated with adult neurodegenerative diseases (e.g., Alzheimer’s disease). Cell-to-cell communication via gap junctions formed by hexameric assemblies of Cxs, known as connexons, is believed to be a crucial component in developmental regulation. As for Panxs, despite being topologically similar to Cxs, they predominantly seem to form channels connecting the cytoplasm to the extracellular space and, despite recent research into Panx1 (Pannexin 1) expression in different regions of the brain during the embryonic phase, it has been studied to a lesser degree. When it comes to the nervous system, Cxs and Panxs play an important role in early stages of neuronal development with a wide span of action ranging from cellular migration during early stages to neuronal differentiation and system circuitry formation. In this review, we describe the most recent available evidence regarding the molecular and structural aspects of Cx and Panx channels, their role in neurodevelopment, congenital and adult neurological diseases, and finally propose how pharmacological modulation of these channels could modify the pathogenesis of some diseases.
Gait Analysis with Wearables Is a Potential Progression Marker in Parkinson’s Disease
Authors: Zhu Sha , Wu Zhuang , Wang Yaxi , Jiang Yinyin , Gu Ruxin , Zhong Min , Jiang Xu , Shen Bo , Zhu Jun , Yan Jun , Pan Yang , Zhang Li
Published: September 2022

Gait disturbance is a prototypical feature of Parkinson’s disease (PD), and the quantification of gait using wearable sensors is promising. This study aimed to identify gait impairment in the early and progressive stages of PD according to the Hoehn and Yahr (H–Y) scale. A total of 138 PD patients and 56 healthy controls (HCs) were included in our research. We collected gait parameters using the JiBuEn gait-analysis system. For spatiotemporal gait parameters and kinematic gait parameters, we observed significant differences in stride length (SL), gait velocity, the variability of SL, heel strike angle, and the range of motion (ROM) of the ankle, knee, and hip joints between HCs and PD patients in H–Y Ⅰ-Ⅱ. The changes worsened with the progression of PD. The differences in the asymmetry index of the SL and ROM of the hip were found between HCs and patients in H–Y Ⅳ. Additionally, these gait parameters were significantly associated with Unified Parkinson’s Disease Rating Scale and Parkinson’s Disease Questionnaire-39. This study demonstrated that gait impairment occurs in the early stage of PD and deteriorates with the progression of the disease. The gait parameters mentioned above may help to detect PD earlier and assess the progression of PD.