bluuebunny/crossref_metadata_2025 huggingface dataset:
Platelet Dysfunction in Cardiopulmonary Bypass: An Experimental Comparative Study between a Centrifugal and a New Pulsatile Pump
Authors: Herreros J. , Berjano E.J. , Más P. , Padrós C. , Sales-Nebot L. , Vlaanderen W. , Díaz P. , Páramo J.A. , Rábago G. , Mercé S.
Published: January 2018

The aim of this investigation was to study the effect of a new pulsatile pump for extracorporeal circulation (ECC) on platelet count and platelet function with respect to a Biomedicus centrifugal pump. Thirteen pigs, 8 in the pulsatile group (PG) and 5 in the centrifugal group (CG), underwent a partial extracorporeal circulation lasting 3h. The animals were sacrificed 3h post-ECC. The platelet study was both quantitative (platelet count) and qualitative (platelet function analysis) by assessing the closure time (CT) with a PFA- 100 system. The decrease in platelet number from basal to 3h post ECC was only significant in CG (p = 0.009). The platelet function was impaired in both groups, but the value of CT with col/ADP increased significantly only in CG (p < 0.001). The increase of CT with col/EPI was greater in CG (p = 0.07) than in PG (p = 0.2). The results indicated that the new pulsatile pump preserves platelets quantitatively and qualitatively well compared to a Biomedicus pump.
The Ex-Vivo-Shunt-Model: Novel Approach for Assessing the Thrombogenicity of Vascular Implants
Authors: Wilhelmi M.H. , Gratz K.F. , Mischke R. , Von Depka M. , Noske D. , Francis T. , Haverich A. , Mertsching H.
Published: January 2018

Objective

Disadvantages associated with commercially available vascular implants necessitate alternative strategies to develop new vascular prostheses. Although many tissue characterizing strategies have been defined, no valid test for thrombogenicity exists. Here we introduce a novel concept for thrombogenicity testing of vascular implants

Methods

Silastic tubes were implanted into the carotid arteries of 12 sheep. After placing these shunts, tc99m-labeled platelets were administered and test-vessels were put in between the shunts. Native autologous (n=6), as well as native / acellularized allogeneic (n=6/n=6), and xenogeneic (n=6/n=6) carotid arteries and allogeneic (n=6/n=6) and xenogeneic (n=6/n=6) carotid arteries re-seeded with allogeneic endothelial-cells, fibroblasts and myocytes were evaluated. Number and time course of intra-operatively deposited platelets were evaluated with a Geiger-counter; certain areas of platelet deposition located, envisioned and characterized by a γ-camera and scanning electron-microscopy afterwards.

Results

Counter results revealed no significant different platelet depositions when comparing silastic tubes with either autologous or allogeneic native carotid arteries. However, starting 5 minutes after placement, acellularized/reseeded allogeneic (p=0.001/p=0.00004), and xenogeneic (p=0.0001/p=0.01) carotid arteries showed significantly more platelet depositions than native autologous carotides. Moreover, it was possible to show that almost no platelets adhere to native vessels or silastic tubes, thus proving the test method itself.

Conclusion

The Ex-Vivo-Shunt-Model is a valid method to measure and envision the intrinsic thrombogenicity of vascular implants.
The Prospective Pediatric Continuous Renal Replacement Therapy (ppCRRT) Registry: Design, Development and Data Assessed
Authors: Goldstein S.L. , Somers M.J.G. , Brophy P.D. , Bunchman T.E. , Baum M. , Blowey D. , Mahan J.D. , Flores F.X. , Fortenberry J.D. , Chua A. , Alexander S.R. , Hackbarth R. , Symons J.M.
Published: January 2018

Many issues plague the pediatric ARF outcome literature, which include data only from single center sources, a relative lack of prospective study, mixture within studies of renal replacement therapy modality without stratification and inconsistent use of methods to control for patient illness severity in outcome analysis. Since January 2001, the Prospective Pediatric CRRT (ppCRRT) Registry Group has been collecting data from multiple United States pediatric centers to obtain demographic data regarding pediatric patients who receive CRRT, assess the effect of different CRRT prescriptions on circuit function and evaluate the impact of clinical variables on patient outcome. The aim of the current paper is to describe the ppCRRT Registry design, review the decision process and rationale for the options chosen for the ppCRRT format and discuss the analysis plan and future projects envisioned for the ppCRRT Registry.
Reverse Transcriptase/Polymerase Chain Reaction Analyses of Hemodialysis Ultrafiltrates and Sera of Hepatitis C Virus Positive Patients
Authors: Simjanovska L.J. , Porcu K. , Amitov V. , Efremov G.D. , Polenakovic M.
Published: January 2018

The hepatitis C virus is a highly prevalent infection among chronic dialysis patients and represents one of the major problems of hemodialysis units. Hepatitis C virus transmission occurs either by blood transfusion or nosocomially. One of the proposed pathways of nosocomial transmission of the hepatitis C virus is cross-contamination through the dialysis procedure.

In an effort to elucidate whether the hepatitis C virus may pass across the hemodialysis membrane, we have performed analyses of ultrafiltrates collected in different stages of hemodialysis treatments, using different types of hemodialysis membranes and different types of dialysis machines. Samples collected from the dialysis compartment during the rinsing of the blood compartment at the end of the hemodialysis treatment were also analyzed.

The hepatitis C virus was found in 17 out of 58 ultrafiltrate samples taken at different times of the hemodialysis treatment. Moreover, the hepatitis C virus was present in 15 out of 17 samples collected from the dialysate compartment during the saline solution rinsing step of the blood compartment. The presence of the hepatitis C virus had no strict correlation with the type of dialysis membrane or with the type of dialysis machine. Although the results suggest that the passage of the hepatitis C virus during the hemodialysis treatment is multi-factorial and case- specific, the most critical point is when the blood is flushed out with physiological saline.
Retrospective Comparison of 5 different Methods for Long-Term LDL-Apheresis in 20 Patients between 1986 and 2001
Authors: Krebs A. , Krebs K. , Keller F.
Published: January 2018

Purpose

To compare long-term efficacy and biocompatibility of the 5 most commonly applied LDL-apheresis techniques using a specifically modified calculation method of the area under the curve (AUC) for laboratory parameters.

Design

Retrospective long-term analysis of 20 patients with homozygous or severe heterozygous familial hypercholesterolemia.

Procedures

The following 5 extra-corporeal LDL-apheresis methods were compared: IMAL (Immuno Adsorption of Lipoproteins), DSA (Dextran Sulphate Adsorption), HELP (Heparin Induced Extra-corporeal LDL Precipitation), DALI (Direct Adsorption of Lipoproteins), MDF (Membrane Differential Filtration).

Main outcome measures

AUC derived plasma concentrations (CAUC) of lipoproteins between two apheresis procedures and their long-term course. Comparison of biocompatibility and efficacy concerning the LDL-C target of < 2.6 mmol/L of 5 apheresis techniques. Progression of atherosclerosis in patients with severe hypercholesterolemia.

Main findings

The means of AUC derived average plasma concentrations (CAUC) of all treatment intervals were for LDL-C and the LDL/HDL ratio as follows: IMAL (5.59 mmol/L; ratio 4.1), DSA (3.03 mmol/L; ratio 2.0), HELP (4.06 mmol/L; ratio 2.2), DALI (3.83 mmol/L; ratio 3.3), MDF (3.26 mmol/L; ratio 3.2). Coronary heart disease and cardiac events (myocardial infarction, PTCA/ stent implantation, CABG) progressed in only 2 patients whereas atherosclerosis manifestations (sclerosis abdominal aorta, carotid artery stenosis, peripheral vascular disease) worsened in 13 patients. Mean ergometric capacity improved from 112 to 118 Watt.

Conclusions

All 5 apheresis methods (IMAL, DSA, HELP, DALI, MDF) proved to be safe and suitable for long-term treatment in patients with severe hypercholesterolemia. The introduction of the CAUC revealed that the target of LDL-C < 2.6 mmol/L was not achieved with regard to the time averaged concentration (CAUC).
Numerical Simulation of the Hepatic Circulation
Authors: Van Der Plaats A. , 'tHart N.A. , Verkerke G.J. , Leuvenink H.G.D. , Verdonck P. , Ploeg R.J. , Rakhorst G.
Published: January 2018

Availability of donor livers and the relatively short preservation time limit the success of liver transplantation. The use of hypothermic machine perfusion could pave the way for expansion of the donor pool. To better define optimal settings of such a device, the feasibility of using a numerical simulation model of the hepatic circulation is determined.

Hemodynamics in the hepatic arterial, portal venous and hepatic venous compartments of the hepatic vascular tree was modelled using an electrical analogue. Calculated pressure and flow profiles throughout the liver were in accordance with physiologic profiles in the total circulatory system. Comparison of calculated flow values with normal control values showed a discrepancy that was explained by inaccurate diameter input data. Until more precise methods for determining vascular dimensions become available, redefining vessel diameter makes the simulation model perfectly suitable for predicting influences of temperature and/or viscosity on hepatic hemodynamics and is thereby an excellent tool in defining optimal settings for our hypothermic liver perfusion system.
Effect of Left Ventricular Assist Device on Circulatory Autonomic Nervous Activity
Authors: Tsutsui T. , Takiya R. , Jikuya T. , Shigeta O. , Sakakibara Y. , Sankai Y.
Published: January 2018

Purpose

For evaluation of the autonomic nervous system (ANS) activity of patients with mechanical circulatory support including LVAD, IABP and PCPS, arterial pressure variability (APV) was examined. APV is the power density values of pressure signals. High frequency (0.15 to 0.4 Hz) and low frequency (0.04 to 0.15 Hz) components of APV are regarded as a function of the autonomic nervous system.

Methods

Arterial pressure signals were acquired every 2 hours at 100 Hz for 6 minutes. Power spectral analysis was carried out, and APV was observed in 18 patients with IABP and/or PCPS. APV was also observed in 3 patients with LVADs (Xemex, Zeon Medical, Japan) before their discharge from ICU for transfer to another hospital for cardiac transplantation.

Results

Although the value gradually increased in 18 patients with IABP and/or PCPS, sustained low values of LFAnorm of APV in 6 patients were related to prolonged mechanical support, and 4 patients among them died consequently. The normalized power spectral component with low frequency (LFAnorm) was maintained at relatively high values in 3 patients with LVADs.

Conclusion

The study results suggest that power spectral analysis of systemic arterial pressure offers a reasonable means for the evaluation of the severity of patient cardiovascular condition and for the prediction of clinical outcome. It also suggested that LVAD support exerts a favorable effect on ANS in patients with severe heart failure before cardiac transplantation.
Treatment of Dyslipidemia in Chronic Kidney Disease
Authors: Stefanovic V. , Milojkovic M.
Published: January 2018

Dyslipidemia is a common feature of various renal diseases. This perturbed lipid metabolism results in accelerated atherosclerosis and increased cardiovascular morbidity and mortality. Treatment of dyslipidemia, in addition to normalization of blood pressure and reduction of proteinuria, could provide additional means to retard the progression of chronic renal insufficiency. Possible therapeutic approaches include mainly dietary and life-style modifications, selective use of some technical components of dialysis systems, and the judicious prescriptions of lipid-lowering drugs. Even with relatively normal lipid and lipoprotein profiles statin therapy seems to prevent atherogenesis acceleration. A wide range of therapeutic interventions, targeting the lipid abnormalities that may develop in chronic renal patients and end-stage renal disease (ESRD) are currently available, though still without convincing evidence based on long-term prospective studies which clearly demonstrate a significant reduction in cardiovascular morbidity and mortality of ESRD patients. However, extensive investigations, concerning the best long-term therapeutic strategy for this high-risk population of patients, are still missing.
The Relationship between Inflammatory Markers, Leptin and Adiponectin in Chronic Hemodialysis Patients
Authors: Lee C-T. , Lee C-H. , Su Y. , Chuang Y-C. , Tsai T-L. , Chen J-B.
Published: January 2018

Chronic inflammation is prevalent in dialysis patients. We investigated the relationship between inflammation and newly identified adipokines: leptin and adiponectin in this population. A total of 129 chronic hemodialysis patients were collected. Serum high sensitivity C-reactive protein (CRP), interleukin-6 (IL-6), leptin and adiponectin levels were determined as well as other metabolic variables. Correlation studies and multiple regression analysis were performed among variables. Our results showed that hemodialysis patients had elevated levels of inflammatory markers, leptin and adiponectin. Diabetic subjects had higher serum CRP and lower albumin levels than non-diabetics. Serum CRP levels were positively correlated with IL-6 levels and negatively correlated with albumin levels. Serum leptin levels were directly related to CRP levels while adiponectin levels were inversely related to CRP levels. A significant negative correlation was observed between serum leptin and adiponectin levels. Serum IL-6 levels were the single independent factor affecting CRP levels. Body mass index can predict both serum leptin and adiponectin levels. We conclude that hemodialysis patients are at an increased risk of chronic inflammation and diabetes patients are even more susceptible to this status. Both serum leptin and adiponectin levels are associated with inflammatory markers. As adipose tissue is the major secreting site of these adipokines, our results suggest that adipose tissue plays an important role in the pathogenesis of chronic inflammation in dialysis patients.
Development of Counterpulsation Algorithm for a Moving-actuator Type Pulsatile LVAD
Authors: Nam K.W. , Chung J. , Choi S.W. , Kim W.E. , Sun K. , Min B.G.
Published: January 2018

A pulsatile left ventricular assist device (LVAD) was used to support the aortic blood pumping function of an injured left ventricle, and as a result helped its recovery. It is important to observe a left ventricle's pumping status and to adjust the operating status of a LVAD to reduce the left ventricle's pumping load and thus to enhance its recovery. To observe the left ventricle's pumping status, an electrocardiogram (ECG) signal is generally used because it is a result of the natural heart's blood pumping function. In this paper, we describe the development of an ECG based counterpulsation control algorithm that prevents simultaneous aortic blood co-pumping by a left ventricle and a moving-actuator type pulsatile LVAD and as a result, reduces the natural heart's pumping load. In addition, to verify the algorithm's applicability for LVAD control we designed three ECG based automatic pump control algorithms that use a developed counterpulsation control algorithm. These algorithms control the operating status of a LVAD automatically and, at the same time, maintain a counterpulsing status. The results of in vitro experiments show that the counterpulsing effect between a left ventricle and a LVAD was successfully produced and that the newly designed automatic pump control algorithms met their own control purposes with a counterpulsing effect.
Bone Biopsy as a Diagnostic Tool in the Assessment of Renal Osteodystrophy
Authors: Spasovski G.B.
Published: January 2018

When renal disease develops, mineral and vitamin D homeostasis is disrupted, resulting in diverse modifications in bone cells, bone structure and the rate of bone turnover. In end stage renal failure (ESRF) when patients require chronic maintenance dialysis, nearly all of them have abnormal bone histology known as renal osteodystrophy (ROD). Moreover, survival rates of patients on dialysis have increased because of therapeutic improvement and the resultant increase in duration of dialysis has led to a further rise in renal osteodystrophy. Because metabolic bone disease can produce fractures, bone pain, and deformities late in the course of the disease, prevention and early treatment are essential. Serum PTH and various bone markers are commonly used to assess bone changes in ESRF patients, but the diagnosis of underlying bone disease is still rather uncertain. To date, bone biopsy is the most powerful and informative diagnostic tool to provide precise information on the type and severity of renal osteodystrophy, and on the presence and amount of aluminum and strontium deposited in the bone. Bone biopsy is not only useful in clinical settings but also in research to assess the effects of therapies on bone. Although considered an invasive procedure, bone biopsy has been proven to be safe and free from major complications, but the operator's experience and skill is important in further minimizing morbidity. Alternatives to bone biopsy continue to be sought, but the non-invasive bone markers have not been proven to be sufficient in diagnostic performance related to bone turnover, mineralization process and bone cell abnormality. Hence, transiliac bone biopsy remains the gold standard for the diagnosis of renal osteodystrophy.
Influence of Differences in Body Weight and Volume Management on Experimental Results in Porcine Models
Authors: Schnoor J. , Schreck R. , Baumert J.H. , Grosse-Siestrup C. , Rossaint R. , Unger J.K.
Published: January 2018

Background

In contrast to humans, young pigs naturally have a low COPpl (12–16 mmHg versus 22–26 mmHg in young humans). Thus, behavior occurring when volume management is performed similar to human medicine might be different. Potentially underestimated intra- to extravascular fluid and solute shifts could influence time course of variables investigated. That is why we studied whether differences in the basic protocol for infusion therapy and different levels of COP - according to age or body weight, respectively - impair reproducibility and reliability of experimental results.

Methods

Group A [n=6, lower body weight (LBW < 22 kg)] was treated with unrestricted infusion rates (UIR) adjusted to maintain a constant blood pressure; group B [n=6, higher body weight (HBW > 28 kg)] was treated with a restricted continuous infusion rate for fluid balance (RIR); group C (n=6) combined HBW and UIR protocol. Blood pressure, plasma solutes, diuresis, and peritoneal fluid were analysed. Statistical analysis was performed using Kruskal-Wallis test and Wilcoxon test.

Results

UIR-treated pigs with LBW (group A) developed ascites and demonstrated time dependent decreases of plasma solute concentrations whereas in pigs of group C mainly diuresis was increased and subcutaneous edema occurred. None of the protocols enabled constant blood pressure.

Discussion

In young pigs (LBW), an adaptive volume substitution using crystalloids to standardize blood pressure may induce fluid extravasation; in turn data may not show statistical significance, stable hemodynamics may not be achieved and changes in plasma solute concentrations may lead to false interpretations. RIR-strategy provided reproducible, plausible results and thus should be recommended in combination with pigs >29 kg BW for the use of porcine models.
Evaluation of the Direct Toxicity of Trioctyltrimellitate (TOTM), di(2-ethylhexyl) Phthalate (DEHP) and their Hydrolysis Products on Isolated Rat Hepatocytes
Authors: Kambia K. , Dine T. , Gressier B. , Dupin-Spriet T. , Luyckx M. , Brunet C.
Published: January 2018

Plasticizers are added to polyvinyl chloride (PVC) to confer flexibility to the polymer. Di-(2-ethylhexyl) phthalate (DEHP) is the most commonly used of them. However, due to its non covalent bond to the PVC, DEHP tends to vaporize easily. A significant exposure has been recorded in dialyzed patients since medical tubings. Most animal species metabolize DEHP rapidly into mono-ethylhexyl phthalate (MEHP) and 2-ethylhexanol (2-EH). Because of the suspected toxicity of DEHP, an alternative plasticizer, trioctyltrimellitate (TOTM) has aroused increasing interest. The aim of this study was to determine on isolated rat hepatocytes in vitro, the direct hepatotoxic potential of both DEHP and TOTM and their hydrolytic products. To evaluate the possible toxic liver risk resulting from exposure to DEHP and TOTM, isolated rat hepatocytes were incubated with either DEHP, TOTM, MEHP or their common metabolite (2-EH) for 3 hours. Cell viability was periodically estimated thanks to trypan blue tests (15 - 180 min). The activity of lactate dehydrogenase (LDH) was also monitored (1h, 2h, 3h). The results obtained with trypan blue test and with direct LDH activity measurements, were satisfactorily correlated. Hepatocytes treated with both plasticizers and metabolites on the one hand, and the controls (untreated suspension) on the other hand, showed important differences as for cell viability. The acute toxicity on hepatocytes is mainly due to MEHP. Among DEHP, TOTM, MEHP, 2-EH and after intraperitoneal injection of those compounds, only DEHP and MEHP were able to induce a significant hydrogen peroxide (H2O2) production by the rat hepatocytes. These observations enable us to confirm the hypothesis according to which DEHP and MEHP cause an imbalance between the synthesis and the degradation of H2O2. Our results suggest a short-term in vitro cytotoxicity of MEHP. Even if trypan blue and LDH tests offered good results and were easily branded, further assays as well as MTT-tests should performed in order to confirm the cytotoxicity of the compounds tested.
Morphological and Functional Alterations in Glycerol Preserved Rat Aortic Allografts
Authors: Fahner P.J. , Idu M.M. , Legemate D.A. , Vanbavel E. , Borstlap J. , Pfaffendorf M. , Van Marle J. , Van Gulik T.M.
Published: January 2018

Glycerol preservation is an effective method for long-term preservation of skin allografts and has a potential use in preserving arterial allografts. We evaluated the effect of glycerol concentration and incubation period on vessel-wall integrity of rat aortic allografts. No significant differences were measured in breaking strength (2.3 ± 0.3 N) and bursting pressure (223 ± 32 kPa) between standard glycerolized and control segments (1.7 ± 0.3 N, 226 ± 17 kPa). Isometric tension measurements showed complete lack of functional contraction and relaxation capacity in allograft segments prepared according to all preservation protocols. Morphologically, thickness of the vessel-wall media diminished after preservation using low (30/50/75 %) or high (70/85/98%) concentrations of glycerol, as compared to control segments (i.e. 81 ± 2.4 μm, 95 ± 5.6 μm and 125 ± 3.5 μm, respectively). Confocal microscopy and Fourier analysis demonstrated that vascular collagen and elastin bundle orientation had remained unaltered. Electron microscopy showed defragmentation of luminal endothelial cells.

In conclusion, glycerol preservation of rat aorta resulted in an acellular tissue matrix, which maintained biomechanical integrity and extracellular matrix characteristics. The next step in the investigation will be to test the concept of glycerol preservation of arterial allografts in a vascular transplantation model.
Contrast Nephropathy, Pathophysiology and Prevention
Authors: Straaten H.M. OUDEMANS-VAN
Published: January 2018

Contrast nephropathy is a common cause of iatrogenic acute renal failure. Its incidence rises with the growing use of intra-arterial contrast in diagnostic and interventional procedures. Aim of the present review is to summarize the knowledge about pathophysiology and prevention. Nephrotoxicity is related to osmolality, dose and route of the contrast and only occurs in synergy with other factors, such as previous renal impairment and cardiovascular disease. With an interplay of these factors, contrast nephropathy has an impact on morbidity and mortality. Pathophysiological mechanisms are intrarenal vasoconstriction, leading to medullary ischemia, direct cytotoxicity, oxidative tissue damage and apoptosis. Several measures are of proven benefit in patients at risk. Among them are discontinuation of potentially nephrotoxic drugs, hydration, preferably with isotonic sodium bicarbonate, use of low osmolal contrast, oral or intravenous N-acetylcysteine and intravenous theophylline. In patients with severe cardiac and renal dysfunction undergoing cardiac interventions, periprocedural hemofiltration may be considered.
Prosthetic Devices Shaped as Tubular Chambers for the Treatment of Large Diaphyseal Defects by Guided Bone Regeneration
Authors: Aldini N. Nicoli , Fini M. , Giavaresi G. , Guzzardella G.A. , Giardino R.
Published: January 2018

Guided tissue regeneration is based on the hypothesis that the different tissues have unequal abilities to penetrate a wounded area during the healing process. The use of a device acting as a chamber allows the growth of a particular tissue and prevents the ingrowth of other tissues which impair the healing process. At the same time the chamber protects and maintains in situ the intrinsic growth factors so that they may perform their specific activity. Guided tissue regeneration currently plays a well-recognized role mostly in dentistry and peripheral nerve surgery but interesting perspectives have also opened up in orthopedics. Considering the possibility of using guided bone regeneration in the repair of diaphyseal bone defects, this updated survey highlights some critical points and pathways related to the state-of-the-art of this promising procedure, focusing particularly on the properties of the material to make the tubular chamber, the use of osteopromotive factors and the most appropriate animal model to be used for the experimental evaluation.
Theoretical Morphological Approach to Simple Peritoneal Sclerosis
Authors: Di Paolo N. , Gaggiotti E.
Published: January 2018

The term peritoneal sclerosis can be applied to a vast range of peritoneal alterations. At one end of the range we have the slight peritoneal sclerosis constantly associated with peritoneal dialysis, which may be defined as simple sclerosis. Its clinical impact is slight.

The role of glucose in determining peritoneal sclerosis is supported by morphological studies and therefore backed by much biochemical and immunological data.
Histological and Functional Characteristics of Peritoneal Membrane in Peritoneal Sclerosis of Pd Patients
Authors: Bertoli S.V. , Buzzi L. , Ciurlino D. , Maccario M. , Traversi L. , Martino S. , Procaccio M.
Published: January 2018

Background

Function and structure of peritoneal membrane (PM) are impaired on peritoneal dialysis (PD). Peritoneal sclerosis is a common finding in peritoneal biopsies (PB) of PD patients. The aim of this study was to examine the impact of peritoneal sclerosis on peritoneal function and clinical parameters in PD patients submitted to peritoneal biopsy.

Methods

A PB was performed on 31 PD patients during catheter removal due to malfunction or after drop-out from treatment. For each patient PM transport was evaluated by the last peritoneal equilibration test before PB. Each daily glucose load was calculated. Tissue was formalin-embedded and stained for histological and immunohistochemical studies.

Results

Patients with submesothelial sclerosis and those with impairment of submesothelial basement membrane and subendothelial vascular membrane were submitted to a larger daily glucose load. Peritoneal sclerosis > 50 microns was more frequent in high transporters, who were exposed to larger daily glucose load compared to medium-high transporters. Mesothelial loss is correlated to peritoneal sclerosis and vascular injuries.

Conclusions

Peritoneal sclerosis is not constant in PD patients: it is related to the loss of mesothelium integrity, to the daily glucose load of PD treatment and to vascular injuries, but apparently not to the presence of inflammatory infiltrate. It remains a matter of debate how much the peritoneal sclerosis modifies the function of PM and how new more biocompatible PD solutions could reduce PM injury.
The Pathogenesis and Therapeutic Option of Encapsulating Peritoneal Sclerosis
Authors: Kawanishi H.
Published: January 2018

Since the first peritoneal dialysis (PD) patients with encapsulating peritoneal sclerosis (EPS) were reported in 1980, EPS has been considered primarily a fatal complication. The incidence of EPS in PD patients has been reported to be from 0.7% to 7.3%, and the rate appears to be higher in patients receiving long-term treatment. Most data from Japan has shown an overall incidence of 2.5% with an evident negative effect of increasing duration of PD, which also augments mortality. Since EPS occurred after withdrawal from PD in more than half of the patients, strict monitoring is necessary when a long-term PD patient is withdrawn from PD. Maintaining patients on standard PD for more than 8 years using conventional solutions is associated with a substantial risk for development of EPS. Appropriate treatment according to the disease stage is most important in EPS treatment. Therefore, when examining a PD patient complaining of gastrointestinal symptoms, the possibility of EPS has to be kept in mind. Basic therapeutic tactics for EPS include an appropriate use of steroids. If the state of bowel obstruction persists, laparotomy and enterolysis should be performed to obtain complete cure. It is now recognized that EPS is not a fatal complication of PD.
A Mathematical Approach to the Evaluation of Sediment Transport Capacity in the Human Urinary Tract
Authors: Calomino F. , Di Paolo N. , Palma G. , Gaggiotti E.
Published: January 2018

Background

It is not yet clear whether forced diuresis is useful for flushing out of the urinary tract, especially in cases of renal calculi or infections. A series of experiments conducted initially in vitro and then in vivo in rabbits, using sand and small glass spheres, showed that sediments can be eliminated from the urinary tract by forced diuresis. When distilled water was used, the effect was greater, both as weight of sand and as number of spheres eliminated, than when normal saline was used. The different characteristics of the two liquids gave rise to different intrapelvic pressures for a given flow.

Materials and Methods

Intrapelvic pressures, and liquid and solid discharges in vitro and in rabbits were used in a fluid mechanics analysis of the phenomenon to obtain non-dimensional formulae so that the results in rabbits could be extended to humans.

Results

The results made it possible to evaluate solid transport capacity induced in a healthy human of given weight by drinking a given quantity of water in an hour.

Conclusions

The mathematical model makes it possible to determine the quantity of water a patient must drink to induce a transport force sufficient for urinary tract wash-out.
Design and in Vitro Performance of a Novel Bileaflet Mechanical Heart Valve Prosthesis
Authors: Medart D. , Schmitz C. , Rau G. , Reul H.
Published: January 2018

Design and in vitro performance of a novel bileaflet mechanical heart valve prosthesis are presented. The novel heart valve exhibits three main design characteristics: (i) The leaflets form a Venturi passage in open position. Thus, a beneficial pressure distribution is obtained and the leaflets are stabilised in opened position. (ii) The orifice inlet is nozzle-shaped. Flow is convectively accelerated and flow separation at the orifice inlet is avoided. (iii) The hinge design facilitates an additional axial movement of the leaflets and leads to a self cleaning effect and enhances washout of the hinges. The design of the leaflet hinges is of main importance for the functional reliability and durability of mechanical heart valves.

After manufacturing first prototypes from titanium and polymeric materials the hydrodynamic performance was evaluated according to ISO 5840 and FDA guidelines. Hydrodynamic performance is comparable with the results of commonly available bileaflet mechanical heart valve prostheses. Initial durability tests showed suitable material couples for further long term studies.
Feasibility of Continuous CO2 Removal with Hydrophilic Membranes at Low Blood Flow Rates
Authors: Czermak P. , Razcuhn B. , Walz M. , Catapano G.
Published: January 2018

In the conventional treatment of acute respiratory distress syndrome (ARDS), high O2 concentrations and mechanical ventilation may damage the lung tissue. Extracorporeal membrane oxygenation limits damage, provides the needed O2 supply and improves survival of ARDS neonates, but not of adults. Hydrophilic membranes used in hemodialysis are more non-thrombogenic and biocompatible than those used in blood oxygenation, but their O2 transport capacity is not as high. In recent years, CO2 removal at low blood flow rates combined with apneic oxygenation and low frequency ventilation has proved promising in the treatment of ARDS. This approach makes O2 supply across ECMO membranes unnecessary; it also makes hydrophilic membranes candidates for extracorporeal CO2 removal to minimize anticoagulation and immune system activation. This paper reports on the in vitro capacity of hydrophilic polysulphone membranes to remove CO2 from carbonated pig blood into an oxygen-rich gas stream. Experiments were performed on clinical-size dialysis modules and their capacity to remove CO2 as a function of blood flow rate and membrane surface area was investigated. Membranes effectively removed CO2, more so at increasing blood flow rates and membrane surface areas, at rates of up to 15% of the CO2 metabolic production rate. The specific CO2 removal rate was comparable to that of blood oxygenators equipped with microporous hydrophobic membranes. It is concluded that CO2 removal from slowly flowing blood with hydrophilic membranes is feasible.
Comparative Analysis of Frozen and Acellularized Vascular Xenografts
Authors: Kim W.G. , Seo J.W. , Yang J.H
Published: January 2018

Background

We implanted frozen and acellularized porcine xenograft vessels as small-diameter arterial grafts in goats and comparatively analyzed the explanted grafts by gross observation and by light microscopy at predetermined periods.

Materials and Methods

Porcine carotid arteries were harvested and immediately stored within a tissue preservation solution at −70°C in a freezer designated for frozen xenograft vessels. The acellularized xenograft vessels were prepared with NaCl-SDS solution and stored frozen until use. One pair of porcine xenograft vessels were used to compare the frozen and acellularized grafts in the bilateral carotid arteries in one goat. The grafts were implanted for one, 3, and 6 months in three animals. Periodic ultrasonographic examinations were performed during the observation period. Explanted grafts were analyzed by gross observation, and by light microscopy.

Results

All animals survived the experimental procedure without specific problems. Ultrasonographic examinations showed excellent patency in all grafts during the observation period. Gross observations revealed nonthrombotic patent smooth lumens. Microscopic examinations of the explanted grafts showed satisfactory cellular reconstruction to the 6-month stage. Although more inflammatory responses were observed in the early phase of implantation of frozen xenografts than of acellularized xenografts, there was no evidence of significant rejection of the frozen xenografts.

Conclusion

These findings suggest that porcine vessel xenografts, regardless of them being acellularized or simply frozen xenografts, can be acceptably implanted in goats as a form of small-diameter vascular graft.
Dialytic Performance Evaluation of Rexeed™: a New Polysulfone-based Dialyzer with Improved Flow Distributions
Authors: Brendolan A. , Nalesso F. , Fortunato A. , Crepaldi C. , De Cal M. , Cazzavillan S. , Cruz D. , Techawathanawanna N. , Ronco C.
Published: January 2018

New dialyzers are designed to optimize the convective and diffusive components of solute transport. Asahi Kasei Medical Co., Ltd. has developed a new high flux dialyzer series called Rexeed™ with improved flow distributions. We evaluated the in vivo dialytic performance of two dialyzers of the Rexeed™ series: Rexeed-18A and Rexeed-25A (1.8 m2 and 2.5 m2).

We calculated the clearance for urea, creatinine, phosphate and b2-microglobulin both in high flux dialysis (HFD) and in 15 liter postidiluitional on-line hemodiafiltration (HDF) mode.

With n=3 patients in high flux HD at blood flow 450, 400, 350 and 250 ml/min we found remarkably high clearance for urea (347±4%,305±0%,288±5%,230±3%, for Rexeed-18A and 361±3%,329±0%,313±1%,234±3% for Rexeed-25A), creatinine (282±10%,234±0%, 221±8%, 174±8%, for Rexeed-18A and 276±6%,245±0%,226±9%,172±13% for Rexeed-25A), phosphate (347±0%,316±0%,275±4%,202±16%, for Rexeed-18A and 364±3%,365±0%,286±3%,224±2% for Rexeed-25A) and b2-microglobulin (133±21%,124±0%,118±12%,98±11%, for Rexeed-18A and 159±8%,169±0%,157±8%,129±7% for Rexeed-25A)

With n=2 patients in HDF at blood flow 300 ml/min we found remarkably high clearance for urea (268±2%, for Rexeed-18A and 283±2% for Rexeed-25A), creatinine (183±6% for Rexeed-18A and 205±9% for Rexeed-25A), phosphate (245±3%, for Rexeed-18A and 270±2% for Rexeed-25A) and b2-microglobulin (166±12%, for Rexeed-18A and 192±4% for Rexeed-25A).

Our preliminary evaluation describes the characteristics and the performances of a new polysulfone-based hemodialyzer series called Rexeed™. Several innovative features have been implemented by the manufacturer. These constructive approaches seem to have produced a positive effect on the dialyzer performance at the bedside.
Nanocapsules as Drug Delivery Systems
Authors: Mayer C.
Published: January 2018

Dispersed polymer nanocapsules can serve as nano-sized drug carriers to achieve controlled release as well as efficient drug targeting. The dispersion stability and the primary physiological response are mainly determined by the type of the surfactant and the nature of the outer coating. Their release and degradation properties largely depend on the composition and the structure of the capsule walls. Another important criterion is the capsule size, where an optimum is generally seen for radii ranging between 100 and 500 nm. Nanocapsules can be prepared by four principally different approaches: interfacial polymerization, interfacial precipitation, interfacial deposition, and self assembly procedures. All these procedures offer their individual advantages and disadvantages when it comes to the design of optimized drug carrier systems. The most important capsule parameters such as capsule radius distribution, the capsule surface, the thickness and the permeability of the capsule membrane and its thermal or chemical decomposition, are discussed and examples are shown. In combination with efficient preparation procedures, nanocapsule dispersions allow for new and promising approaches in many kinds of pharmaceutical therapies.
Implant-Associated Posttraumatic Osteomyelitis: Collateral Damage by Local Host Defense?
Authors: Wagner C. , Obst U. , HÄnsch G.M.
Published: January 2018

Infections following osteosynthesis or total joint replacement, also known as “implant-associated posttraumatic osteomyelitis”, represent a major complication in orthopedic and trauma surgery. While the formation of bacterial biofilms on the implanted osteosynthesis materials is generally accepted as cause of the persistent infection, the molecular mechanisms leading to the progressive and destructive local inflammatory process and eventually to bone degradation, the osteolysis, have not been delineated. Here we provide evidence supporting the hypothesis that it is not the infection per se that causes tissue degradation and osteolysis, but rather the cytotoxic, proteolytic, and proinflammatory effector functions of cells of the host defense, particularly of the infiltrating polymorphonuclear neutrophils.
Do We Have Reliable Biochemical Markers to Predict the Outcome of Critical Illness?
Authors: Schetz M.R. , Van Den Berghe G.
Published: January 2018

Current outcome prediction in critically ill patients relies on the art of clinical judgement and/or the science of prognostication using illness severity scores. The biochemical processes underlying critical illness have increasingly been unravelled. Several biochemical markers reflecting the process of inflammation, immune dysfunction, impaired tissue oxygenation and endocrine alterations have been evaluated for their predictive power in small subpopulations of critically ill patients. However, none of these parameters has been validated in large populations of unselected ICU patients as has been done for the illness severity and organ failure scores. A simple biochemical predictor of ICU mortality will probably remain elusive because the processes underlying critical illness are very complex and heterogeneous. Future prognostic models will need to be far more sophisticated.
Cell Dynamics in the Correct Control of Bone Metabolism Using Natural Treatments
Authors: Mattioli-Belmonte M. , Kyriakidou K. , Lucarini G. , Gorrieri O. , Giavaresi G. , Fini M. , Giardino R. , Amati S. , Suffritti G. , Biagini G.
Published: January 2018

The present study was undertaken in order to assess the efficacy of a commercial product containing calcium and silicon (Osteosil-Calcium®) on cell metabolism. MG-63 osteblast-like cells were cultured in the presence of three different drug concentrations (10, 5 and 2.5 μg/mL). Either serum-free culture and standard culture with serum were investigated. Morpho-functional tests (MTT and ALP), scanning electron microscopy (SEM), microanalysis (EDAX) and time-lapse video microscopy were performed. Cell actin cytoskeletal modification with fluorescence phalloidin staining was also tested. Our data show the in vitro functional efficacy of Osteosil-Calcium® on MG63 cell viability and ALP production. This study demonstrates its positive effect on the metabolism of the single cell and suggests wider uses of this drug in health protection and or in Regenerative Medicine therapies which are currently applied to the elderly
Effect of Hirudin versus Heparin on Hemocompatibility of Blood Contacting Biomaterials: An in Vitro Study
Authors: Kopp R. , Bensberg R. , Kashefi A. , Mottaghy K. , Rossaint R. , Kuhlen R.
Published: January 2018

Hirudin serves as an alternative anticoagulant for extracorporeal blood circulation. Comparing anticoagulation with hirudin (2.5 or 5.0 μg/mL) and heparin (2.0 or 4.0 IU/mL) human blood was circulated in a modified “Chandler System” using PVC-tubes for 2 hours at 37°C. Activation of coagulation (thrombin-antithrombin III-complex, prothrombin fragment 1+2 and D-Dimer), platelet (platelet factor 4 - PF4) and complement systems was analyzed.

Both heparin concentrations and 5.0 μg/dL hirudin led to as significantly less activated plasmatic coagulation as 2.5 μg/dL hirudin. Decreased levels of PF4 and anaphylatoxin C5a (P&0.05) as well as terminal complement complex demonstrated improved hemocompatibility after anticoagulation with heparin in contrast to hirudin.

Because initial coagulation cascade, platelet activation and complement activation is less influenced by hirudin than by heparin, hemocompatibility is more dependent on the characteristics of the biomaterials used. This predestines hirudin as anticoagulant for in vitro studies analyzing hemocompatibility of biomaterials or surface modifications.
Advantages and Disadvantages of Surgical Placement of PD Catheters with Regard to Other Methods
Authors: Stegmayr B.G.
Published: January 2018

Peritoneal dialysis is underused for various reasons. One reason may be problems with insertion of catheters for access. Another reason is the delayed start (break-in period) of about 2 weeks after operation. This review describes various approaches to the insertion of a peritoneal dialysis catheter. The optimal conditions to strive for are given as is an overview of various techniques. This article favours surgical placement while others might prefer other techniques. Described is the use a 3-purse string suture technique that allows immediate start of dialysis after catheter insertion both for acute dialysis indications as well as for acute start in chronic dialysis patients.

A key to lesser complications is to establish a team devoted to the insertions rather than to allow various physicians to perform insertions as a training procedure. An optimal access is one of the important life lines for these patients.
The Self-Locating Catheter: Review and Cost Analysis
Authors: Di Paolo N. , Sansoni E. , Cappelletti F. , Cavatorta F. , Galli S. , Nicolai G.A. , Gaggiotti E.
Published: January 2018

The self-locating catheter invented by Nicola Di Paolo has been increasingly used in Italy and elsewhere since 1994, with about a thousand patients currently implanted every year. Twelve grams of tungsten inserted in the tip of the conventional Tenckhoff catheter during extrusion do not significantly change its form, but suffice to keep the tip firmly in the Douglas cavity.

The validity of the new catheter is confirmed by a multicentric controlled study in a large population of peritoneal dialysis patients. This trial showed that patients with the new catheter have fewer episodes of peritonitis, tunnel infection, cuff extrusion, catheter malfunction, obstruction and leakage.

This paper outlines the present situation and reports a comparative analysis of the costs of Tenckhoff and self-locating catheters.
The Vicenza “Short” Peritoneal Catheter: A Twenty Year Experience
Authors: Dell'Aquila R. , Chiaramonte S. , Rodighiero M.P. , Di Loreto P. , Spanò E. , Nalesso F. , Cruz D. , Kuang D. , Ronco C.
Published: January 2018

Dislocation of peritoneal dialysis catheters is one of the major causes of technique failure. We evaluated 701 Vicenza catheters, implanted since 1985 in 365 males, mean age 53 ± 16 yrs, range 24 - 87, and 336 females, mean age 51 ± 17 yrs, range 21 - 82. The Vicenza catheter is defined “short” since it consists of a classic straight double cuff PD catheter having however an inner segment (the portion located in the peritoneal cavity) much shorter than any other type of catheter. It is implanted in the lower abdomen, just a few centimeters above the pubis. The analysis of our results obtained in a large PD population displayed good device survival at 2 and 5 years (94.3% and 91.5% respectively), a low dislocation rate (4%) and an exit-site infection rate similar to other double cuffed catheters. There was no selection of patients receiving this catheter since from 1985 we have used this catheter in every incident patient. Due to its lower implantation site this catheter demonstrates excellent wearability and good body image acceptance.