bluuebunny/crossref_metadata_2025 huggingface dataset:
CDNF and MANF in the brain dopamine system and their potential as treatment for Parkinson’s disease
Authors: Pakarinen Emmi , Lindholm Päivi
Published: July 2023

Parkinson’s disease (PD) is a progressive neurodegenerative disease characterized by gradual loss of midbrain dopamine neurons, leading to impaired motor function. Preclinical studies have indicated cerebral dopamine neurotrophic factor (CDNF) and mesencephalic astrocyte-derived neurotrophic factor (MANF) to be potential therapeutic molecules for the treatment of PD. CDNF was proven to be safe and well tolerated when tested in Phase I-II clinical trials in PD patients. Neuroprotective and neurorestorative effects of CDNF and MANF were demonstrated in animal models of PD, where they promoted the survival of dopamine neurons and improved motor function. However, biological roles of endogenous CDNF and MANF proteins in the midbrain dopamine system have been less clear. In addition to extracellular trophic activities, CDNF/MANF proteins function intracellularly in the endoplasmic reticulum (ER), where they modulate protein homeostasis and protect cells against ER stress by regulating the unfolded protein response (UPR). Here, our aim is to give an overview of the biology of endogenous CDNF and MANF in the brain dopamine system. We will discuss recent studies on CDNF and MANF knockout animal models, and effects of CDNF and MANF in preclinical models of PD. To elucidate possible roles of CDNF and MANF in human biology, we will review CDNF and MANF tissue expression patterns and regulation of CDNF/MANF levels in human diseases. Finally, we will discuss novel findings related to the molecular mechanism of CDNF and MANF action in ER stress, UPR, and inflammation, all of which are mechanisms potentially involved in the pathophysiology of PD.
Differential impact of resilience on demoralization and depression in Parkinson disease
Authors: de Figueiredo John M. , Zhu Boheng , Patel Amar S. , Kohn Robert , Koo Brian B. , Louis Elan D.
Published: July 2023

Objectives

The objective of this study was to study the interrelations of demoralization, depression, and resilience in patients with Parkinson disease, and, more specifically, to determine if higher resilience in patients with Parkinson disease is associated with lower demoralization, lower depression, or both.

Methods

Outpatients with Parkinson disease (N = 95) were assessed for demoralization, depression, and resilience, as well as sociodemographic, clinical, and treatment-related variables. Bivariable associations, standard regressions, linear regression with copula correction, and correspondence analysis were used to analyze the data.

Results

Although the bivariable association between resilience and depression was statistically significant, the association ceased to be significant when demoralization was taken into consideration in both standard regressions and linear regression with copula correction. By contrast, the association between resilience and demoralization was significant when depression was not taken into consideration and continued to be significant when depression was taken into consideration. Correspondence analysis revealed that low resilience was strongly related to demoralization combined with depression, whereas normal resilience was closely correlated with depression without demoralization.

Conclusion

These results expand our understanding of resilience by suggesting that it is a mechanism evolved to reduce or prevent demoralization and not just depression. Reducing demoralization and strengthening resilience as part of a comprehensive treatment plan are likely to improve the prognosis of Parkinson disease.
Higher metabolic variability increases the risk of depressive disorder in type 2 diabetes mellitus: a longitudinal nationwide cohort study
Authors: An Ji Hyun , Han Kyung-do , Jeon Hong Jin
Published: July 2023

Background and objectives

While variabilities in metabolic parameters (METv) have been linked to adverse health outcomes in type 2 DM, their association with depression is yet to be studied. This research aimed to investigate the association between METv and depressive disorder in patients with type 2 DM.

Methods

The study involved a nationwide cohort of 1,119,631 type 2 DM patients who had undergone three or more serial health examinations between 2005 and 2012. At each visit, body mass index (BMI), fasting glucose (FG), systolic blood pressure (BP), and total cholesterol (TC) were measured and stratified into quartiles, with Q4 being the highest and Q1 the lowest. The risk of depressive disorder was evaluated using Cox proportional hazard regression models, which accounted for METs in the indexes, after adjusting for sex, income status, lifestyle habits, medical comorbidities, DM severity, and baseline levels of BMI, FG, BP, and TC.

Results

During a mean follow-up period of 6.00 ± 2.42 years, 239,477 (21.4%) cases of type 2 DM patients developed depressive disorder. The risk of developing depressive disorder was gradually increased as the number of METv increased (HR 1.18; 95% CI 1.13, 1.23 for the group with the highest METv in all parameters compared to those with the lowest METv in all parameters). In the subgroup analysis, the risk of developing depressive disorder was 43% higher in men (HR 1.43; 95% CI 1.34, 1.51), and 31% higher in those younger than 65 years of age (HR 1.31; 95% CI 1.23, 1.39) in the group with the highest number of METv compared to the group with the lowest number of METv.

Conclusion

In type 2 DM, higher METv was an independent risk factor for depressive disorder. This risk is notably elevated in men and individuals under the age of 65 years.
Trajectories of resilience to acute malnutrition in the Kenyan drylands
Authors: Bhavnani Ravi , Schlager Nina , Reul Mirko , Donnay Karsten
Published: July 2023

Introduction

Insight into the resilience of local food systems—variability driven by climate, conflict, and food price shocks—is critical for the treatment and prevention of child acute malnutrition.

Methods

We use a combination of latent class mixed modeling and time-to-event analysis to develop and test a measure of resilience that is outcome-based, sensitive to specific shocks and stressors, and captures the enduring effects of how frequently and severely children face the risk of acute malnutrition.

Results

Harnessing a high-resolution longitudinal dataset with anthropometric information on 5,597 Kenyan households for the 2016–20 period, we identify resilience trajectories for 141 wards across Kenya. These trajectories—characterized by variation in the duration and severity of episodes of acute malnutrition—are associated with differential risk: (1) some 57% of wards exhibit an increasing trajectory—high household risk despite growing resilience; (2) 39% exhibit chronic characteristics—showing no real signs of recovery after an episode of crisis; (3) 3% exhibit robust characteristics—low variability with low-levels of individual household risk; whereas (4) 1% show a steady decrease in resilience—associated with high levels household risk.

Discussion

Our findings highlight the importance of measuring resilience at the ward-level in order to better understand variation in the nutritional status of rural households.
Identification of Eimeria tenella sporozoite immunodominant mimotopes by random phage-display peptide libraries–a proof of concept study
Authors: Juárez-Estrada Marco A. , Tellez-Isaias Guillermo , Graham Danielle M. , Laverty Lauren , Gayosso-Vázquez Amanda , Alonso-Morales Rogelio A.
Published: July 2023

Introduction

Coccidiosis, caused by parasites of numerous Eimeria species, has long been recognized as an economically significant disease in the chicken industry worldwide. The rise of anti-coccidian resistance has driven a search for other parasite management techniques. Recombinant antigen vaccination presents a highly feasible alternative. Properly identifying antigens that might trigger a potent immune response is one of the major obstacles to creating a viable genetically modified vaccine.

Methods

This study evaluated a reverse immunology approach for the identification of B-cell epitopes. Antisera from rabbits and hens inoculated with whole-sporozoites of E. tenella were used to identify Western blot antigens. The rabbit IgG fraction from the anti-sporozoite serum exhibited the highest reactogenicity; consequently, it was purified and utilized to screen two random Phage-display peptide libraries (12 mer and c7c mer). After three panning rounds, 20 clones from each library were randomly selected, their nucleotide sequences acquired, and their reactivity to anti-sporozoite E. tenella serum assessed. The selected peptide clones inferred amino acid sequences matched numerous E. tenella proteins.

Results and Conclusions

The extracellular domain of the epidermal growth factor-like (EGF-like) repeats, and the thrombospondin type-I (TSP-1) repeats of E. tenella micronemal protein 4 (EtMIC4) matched with the c7c mer selected clones CNTGSPYEC (2/20) and CMSTGLSSC (1/20) respectively. The clone CSISSLTHC that matched with a conserved hypothetical protein of E. tenella was widely selected (3/20). Selected clones from the 12-mer phage display library AGHTTQFNSKTT (7/20), GPNSAFWAGSER (2/20) and HFAYWWNGVRGP (8/20) showed similarities with a cullin homolog, elongation factor-2 and beta-dynein chain a putative E. tenella protein, respectively. Four immunodominant clones were previously selected and used to immunize rabbits. By ELISA and Western blot, all rabbit anti-clone serums detected E. tenella native antigens.

Discussion

Thus, selected phagotopes contained recombinant E. tenella antigen peptides. Using antibodies against E. tenella sporozoites, this study demonstrated the feasibility of screening Phage-display random peptide libraries for true immunotopes. In addition, this study looked at an approach for finding novel candidates that could be used as an E. tenella recombinant epitope-based vaccine.
Conversion From Intravenous In-Hospital Belatacept Injection to Subcutaneous Abatacept Injection in Kidney Transplant Recipients During the First COVID-19 Stay-at-Home Order in France
Authors: Bertrand Dominique , Brunel Mélanie , Lebourg Ludivine , Scemla Anne , Lemoine Mathilde , Amrouche Lucile , Laurent Charlotte , Legendre Christophe , Guerrot Dominique , Anglicheau Dany , Sberro-Soussan Rebecca
Published: July 2023

The first COVID-19 stay-at-home order came into effect in France on 17 March 2020. Immunocompromised patients were asked to isolate themselves, and outpatient clinic visits were dramatically reduced. In order to avoid visits to the hospital by belatacept-treated kidney transplant recipients (KTRs) during the initial period of the pandemic, we promptly converted 176 KTRs at two French transplant centers from once-monthly 5 mg/kg in-hospital belatacept infusion to once-weekly 125 mg subcutaneous abatacept injection. At the end of follow-up (3 months), 171 (97.16%) KTRs survived with a functioning graft, 2 (1.14%) had died, and 3 (1.70%) had experienced graft loss. Two patients (1.1%) experienced acute T cell–mediated rejection. Nineteen patients (10.80%) discontinued abatacept; 47% of the KTRs found the use of abatacept less restrictive than belatacept, and 38% would have preferred to continue abatacept. Mean eGFR remained stable compared to baseline. Seven patients (3.9%) had COVID-19; among these, two developed severe symptoms but survived. Only one patient had a de novo DSA. Side effects of abatacept injection were uncommon and non-severe. Our study reports for the first time in a large cohort that once-weekly injection of abatacept appears to be feasible and safe in KTRs previously treated with belatacept.
Véletlenszám-generátor
Authors: Harangus Katalin , Kakucs András
Published: July 2023

Az oktatás során fontos szerephez jut a szemléltetés: a véletlen folyamatok szemléltetésére, a valószínűségi eloszlások magyarázatára a Galton-deszka mutatkozik alkalmas eszköznek. Mi ezt az eszközt virtuális verziójában alkottuk meg, ami megkönnyíti a kísérleti eredmények statisztikai feldolgozása érdekében végzett adatgyűjtést, és lehetővé teszi a nem-szimmetrikus eloszlások tanulmányozását is. Az eszközön a véletlen folyamatokat szimuláljuk, amihez szükség volt egy véletlenszám-generátorra. Mivel a véletlen számok szoftveres előállítása körül bizonyos kételyek merültek fel, egy hardveres véletlenszám-generátort hoztunk létre, a mikrokontrollerek bemeneti zajára alapozva.
Improved Vehicle Vibration Control through Optimization of Suspension Parameters Using the Response Surface Method and a Non-Linear Programming with a Quadratic Lagrangian Algorithm
Authors: Dai Wei , He Liuqing , Pan Yongjun , Zhang Sheng-Peng , Hou Liang
Published: July 2023

Vibration-control techniques generally fall into two categories: passive methods that optimize the structure of the suspension to absorb any impact from the ground, and active methods that directly control the vertical force of the suspension by hydraulic or electric actuators when the vehicle traverses a bumpy road. In this study, a vibration-control method is described that employs both an optimal controller and suspension parameter optimization. Continuous speed bumps are implemented to simulate more complex and realistic driving conditions. First, a vehicle system is modeled using a semi-recursive multibody formulation, which allows for a more precise description of the longitudinal–vertical dynamics. Then, an optimal control method for vehicle vibration control is introduced. Second, the Latin hypercube design is utilized to analyze the response surface methodology (RSM) model. For suspension optimization, the RSM model and the non-linear programming with a quadratic Lagrangian (NLPQL) algorithm are employed. Thirdly, both passive suspension optimization and active motion control are employed for vibration control. The results indicate that the presented method can effectively control vehicle vibration, decreasing the average vibration by 30.8%. The results suggest that the novel approach can also enhance the ride comfort in autonomous vehicles traversing, e.g., a series of speed bumps.
Experimental and Numerical Investigation on Finned Vortex Reducer in a Rotating Cavity with a Radial Inflow
Authors: He Jian , Luo Xiang , Bai Yang , Song An , Yang Tao
Published: July 2023

In aero-engines, a secondary air system is used to cool the rotor discs and seal cavities between rotor and stator parts. The pressure loss caused by bleed air can be effectively reduced by setting the finned vortex reducer. Thus, the bleed system design can be optimized by researching the flow structure and pressure loss of each section in the cavity with a finned vortex reducer. In this study, the influence of different installation positions of a finned vortex reducer on the pressure loss characteristics was investigated through experimental and numerical simulation methods, focusing on the radial inflow of the secondary air system. The results indicate that the inlet and outlet positions of the fins affect the flow structure in the cavity. The aerodynamic parameters (rotational Reynolds number ReΦ and mass flow rate coefficient Cw), together with the inlet and outlet radii of the fins, influence the pressure loss in the cavity. Considering the swirl ratio β constrained by the fins, a pressure loss model was established, which showed good agreement with the experimentally measured pressure loss. This model reflects the impact of the inlet and outlet positions of the fins on the pressure loss characteristics.
Research on the Level of Agricultural Green Development, Regional Disparities, and Dynamic Distribution Evolution in China from the Perspective of Sustainable Development
Authors: Zhou Feng , Wen Chunhui
Published: July 2023

Green development is a concept of sustainable development, aiming to protect the environment and ecosystems while meeting economic development needs. In the field of agriculture, green development has emerged as a crucial pathway for reconciling the conflicts between agricultural development and ecological conservation. To investigate the level of green development in Chinese agriculture, regional variations, and the evolutionary patterns, this paper is based on the framework of sustainable development theory. This study establishes a comprehensive evaluation system for agricultural green development and applies methods such as entropy-weighted TOPSIS, Dagum’s Gini coefficient, kernel density estimation, Moran’s I index, and Markov chains to analyze the level of agricultural green development, regional disparities, and dynamic evolution in China. The findings of this study reveal that: (1) The overall level of agricultural green development in China is steadily improving, with notable differences in the level of agricultural green development among different regions and provinces. There are significant disparities in agricultural green development between regions, and the overall disparities exhibit a fluctuating downward trend characterized by periods of increase followed by decrease. The regional disparities are identified as the primary cause of the overall disparities in agricultural green development in China. (2) The eight major economic regions in China are experiencing steady development in agricultural green practices, but there are varying degrees of polarization due to different development speeds. (3) This study also highlights a clear spatial positive correlation in the level of agricultural green development in China, with most provinces showing clustering in the first and third quadrants, indicating a “high–high” (H-H) and “low–low” (L-L) agglomeration pattern. (4) The study reveals that the level of agricultural green development in China exhibits a certain degree of stability. Over time, the probability of transitioning from lower-level regions to neighboring higher-level regions increases, and the agricultural green development level in neighboring regions can influence the spatial transfer probability within a given region. Therefore, agricultural green development demonstrates significant spatial dependence.
Temporal Dynamics of Biomarker Response in Folsomia candida Exposed to Azoxystrobin
Authors: Kovačević Marija , Stjepanović Nikolina , Zelić Luca , Lončarić Željka
Published: July 2023

Azoxystrobin (AZO) is widely used to prevent and treat fungal diseases in important crops but can also impact non-target organisms, including mammals, amphibians, aquatic, and soil organisms. Collembolans play important roles in ecosystems as decomposers, fungal feeders, and regulators of microbes. This study aimed to investigate the effects of AZO on Collembola Folsomia candida using a reproduction test and assess biomarker responses over different time intervals (3, 5, 7, 14, and 28 days). Results showed AZO negatively affected reproduction at concentrations of 50, 100, and 200 mg./kg, resulting in decreases of 48.3%, 64.5%, and 81.3%, respectively, compared to the control. Adult survival remained unaffected. The estimated EC50 (reproduction) in artificial soil was 61.28 mg kg−1. Biomarker responses varied with concentration and time. Protein and glycogen concentrations increased with exposure time, while lipid content was affected initially but returned to control levels by day 28. Oxidative stress biomarkers (CAT, SOD, GST, TBARS) indicated AZO induced oxidative stress, intensifying over time. After 28 days, MDA concentrations were significantly elevated compared to the control, suggesting the antioxidant system is overwhelmed which caused damage to lipid membranes. This study showed that azoxystrobin caused negative effects at molecular and population level on non-target species of Collembola.
Rural Displacement and Its Implications on Livelihoods and Food Insecurity: The Case of Inter-Riverine Communities in Somalia
Authors: Osman Alinor Abdi , Abebe Gumataw Kifle
Published: July 2023

This study investigates the phenomenon of forced displacement in Somalia over the past few decades and its implications for the livelihoods and food security of IDP communities. Employing a mixed-method approach, the study draws on various theories to interpret the complex dynamics underlying forced displacement and the subsequent loss of livelihoods. The findings reveal that the drivers of displacement have exhibited variation across different periods, encompassing conflicts, droughts, food scarcity, and political intricacies. Notably, the displacement experienced by inter-riverine communities primarily stems from weak institutions, intensified resource competition, disputes over fertile agricultural land, and conflict and food scarcity. This displacement has resulted in a rapid increase in urban populations and socio-economic crises. Primary data substantiates the severe socio-economic challenges faced by displaced individuals. Such historical perspectives and empirical evidence allow policymakers and stakeholders to better comprehend the multifaceted challenges confronting Somalia. The study underscores the agricultural implications of forced displacement, emphasizing the importance of targeted interventions to revitalize agricultural systems, resolve land disputes, facilitate access to vital resources, and enhance the livelihood conditions of affected communities within Somalia and in similar contexts elsewhere.
Fungicidal Protection as Part of the Integrated Cultivation of Sugar Beet: An Assessment of the Influence on Root Yield in a Long-Term Study
Authors: Jaskulska Iwona , Kamieniarz Jarosław , Jaskulski Dariusz , Radziemska Maja , Brtnický Martin
Published: July 2023

Despite the major role of non-chemical treatments in integrated plant protection, fungicides often need to be applied as a crop protection treatment in sugar beet farming. They should be used based on a good understanding of the requirements and effectiveness of the active ingredients. In 11-year field experiments, the effect that one and three foliar applications of fungicides containing various active ingredients (triazoles, benzimidazoles, strobilurines) had on sugar beet root yields was assessed, depending on various thermal and rainfall conditions. It was found that in eight of the 11 years, foliar application of fungicides increased yields compared to unprotected plants, and three foliar treatments during the growing season were more effective than a single application. The negative correlation of the root yield of fungicidally protected plants with total June rainfall was weaker than the same relationship for unprotected plants. At the same time, the positive correlation between the yield of fungicidally protected sugar beets and average June air temperature was stronger than the same relationship for unprotected plants. The research results indicate the need to conduct long-term field experiments and to continuously improve integrated production principles for sugar beet, especially regarding the rational use of pesticides.
Spatial Pattern of Farmland Transfer in Liaoning Province, China
Authors: Ning Jiachen , Zhang Pingyu , Yang Qifeng , Ma Zuopeng
Published: July 2023

Farmland transfer (FT) is the key to achieving moderate agricultural scale management in China. Clarifying the spatial pattern of FT is important to improve FT strategies. In this study, the multinomial logit model was used to reveal the spatial pattern of FT in Liaoning Province, Northeast China. On this basis, the reasons for its formation were discussed, and suggestions were proposed. According to the statistical results, 39.7% of the sample peasant households participated in FT. Most of them live far from the regional core area. Regression analysis shows that the FT in Liaoning Province has a significant “core-periphery” spatial pattern. As the spatial distance between the residence and the regional core area (SDRRC) increases, the probability of FT rises for peasant households. Specifically, the odds ratios of farmland transfer out and farmland transfer in rise by 0.9% and 0.6% on average, respectively, for each 1 km increase in SDRRC. Widespread concurrent business and the increase in FT fees due to imperfect urbanization are the main reasons for the formation of the spatial pattern. We suggest that the promotion of FT requires high-quality urbanization in central cities, accelerating urbanization in medium and small cities and counties, implementing differentiated FT subsidy standards, and promoting new agricultural scale management models.
Effect of Bioreactor Cultures on the Proliferation and Biological Activity of Protocorm-like Bodies of Dendrobium loddigesii
Authors: Yang Jinfeng , Kwon Yong Soo , Seong Eun Soo , Kim Myong Jo
Published: July 2023

Dendrobium loddigesii has long been used in traditional folk medicine. The purpose of this study was to optimize the culture conditions for its protocorm-like bodies (PLBs) and explore their biological activities. The use of an air-lift bioreactor demonstrated superior PLB proliferation compared to agitated and solid culture methods. The optimal inoculum quantity of 30 g/vessel, cultured for 28 days in the bioreactor, yielded the highest PLB biomass production. Analysis of PLB extracts revealed that the ethyl acetate (EtOAc) extract exhibited the highest levels of flavonoids and alkaloids, as well as potent antioxidant activity demonstrated by DPPH free radical scavenging assay and reducing power. Furthermore, the antiproliferative effects of the PLB extracts were assessed using MTT assays, and the EtOAc extract showed significant efficacy by reducing cell viability by over 60% in the human colon carcinoma cell line SW620 at the highest tested concentration (200 μg/mL). Mechanistic analysis revealed the downregulation of key regulatory apoptosis genes, including survivin, p53, caspase-3, and caspase-9. These results demonstrate the potential of the bioreactor culture method for the efficient production of D. loddigesii PLBs and the biological activities of the EtOAc extract, suggesting its therapeutic potential.
Ethylene Signaling Pathway Genes in Strawberry and Their Expression Patterns during Fruit Ripening
Authors: Zhang Yunting , Deng Meiyi , Gu Xianjie , Guo Chenhui , Chen Yan , Lin Yuanxiu , Chen Qing , Wang Yan , Zhang Yong , Luo Ya , Wang Xiaorong , Tang Haoru
Published: July 2023

Ethylene at least partly regulates some aspects during non-climacteric ripening in strawberry. However, the ethylene signaling pathway genes in the strawberry fruit have not been comprehensively and systematically analyzed. In the present study, 15 FaETRs and 14 FaEIN3/EINs were identified in the octoploid strawberry genome. Subcellular localization analysis predicted that FaETRs and FaEIN3/EINs are respectively localized to the endoplasmic reticulum and the nucleus. The phylogenetic trees showed that FaETRs were classified into two subgroups, while FaEIN3/EINs were divided into three clades, which was supported by gene structure and conserved motif analysis. FaETRs and FaEIN3/EINs could interact with several components, such as CTR1, RTE1, EIN2 and ERF1B, in the ethylene signaling pathway by protein–protein interaction network analysis. Transcriptomic data showed that FaETRs were mainly expressed at the early stage of fruit development in three strawberry cultivars. Additionally, a couple of FaETRs (FaETR2 and FaETR13) and FaEINs (FaEIN2 and FaEIN7) could be induced by 1 μM ABA and inhibited by 100 μM nordihydroguaiaretic acid (NDGA, an ABA biosynthesis blocker). These findings suggested that the FaETR- and FaEIN3/EIN-mediated ethylene signaling pathway might play a role in strawberry fruit ripening.
Evaluation of the Effect of the Vigor of Soybean Seeds Treated with Micronutrients Using X-ray Fluorescence Spectroscopy and Hyperspectral Imaging
Authors: Alves Rafael Mateus , Gomes-Junior Francisco Guilhien , Carmo-Filho Abimael dos Santos , Ribeiro Glória de Freitas Rocha , Rego Carlos Henrique Queiroz , Iost-Filho Fernando Henrique , Yamamoto Pedro Takao
Published: July 2023

Seed treatment with micronutrients is a crucial strategy for providing early seedling supply during development, and is commonly employed in soybean cultivation. However, responses to micronutrient treatment may vary based on seed vigor levels. Therefore, this study aimed to assess the potential of hyperspectral imaging combined with preprocessing and machine learning, compared to X-ray fluorescence spectroscopy, in evaluating the dynamics of micronutrient uptake during the germination of soybean seeds with varying levels of vigor. Two seed lots with differing levels of vigor were utilized for the analysis. The absorption of micronutrients by the seeds was evaluated using X-ray fluorescence spectroscopy (XRF), microprobe X-ray fluorescence spectroscopy (μ-XRF) and hyperspectral imaging (HSI) in two regions of interest (cotyledons and the embryonic axis). Artificial neural network (ANN), decision tree (DT) and partial least squares–discriminant analysis (PLS-DA) classification models, along with the Savitzky–Golay (SG), standard normal variation (SNV) and multiplicative scatter correction (MSC) methods, were employed to determine seed vigor based on the impact of micronutrient treatment. XRF identified higher concentrations of micronutrients in the treated seeds, with zinc being the predominant element. μ-XRF analysis revealed that a significant proportion of the micronutrients remained adhered to the hilum and seed coat, irrespective of seed vigor. The PLS-DA classification model using spectral data exhibited higher accuracy in classifying soybean seeds with high and low vigor, regardless of seed treatment with micronutrients and the analyzed region.
Development of Expressed Sequence Tag–Simple Sequence Repeat Markers Related to the Salt-Stress Response of Kenaf (Hibiscus cannabinus)
Authors: An Xia , Liu Qin , Ying Jinyao , Wei Jiqian , Dong Guoyun , Luo Xiahong , Li Wenlue , Liu Tingting , Zhou Huaping , Zou Lina , Chen Changli
Published: July 2023

Kenaf is one of the most important natural cannabis plants. Molecular marker-assisted breeding is vital for accelerating the breeding process of kenaf. However, the number of kenaf markers is insufficient for molecular marker-assisted breeding. Using transcriptome sequencing data for salt-stressed kenaf plants, the number and distribution of simple sequence repeats (SSRs) and single nucleotide variations (SNVs) in the expressed sequences were determined. The objectives of this study were to elucidate the sequence variations in kenaf genes expressed in response to salt stress and to identify stable and dependable molecular markers. Primers were designed for SSR loci and then EST-SSR molecular markers were generated. The subsequent analyses revealed that 30.50% of the unigenes contained SSR motifs, most of which were single nucleotides followed by trinucleotides and dinucleotides. The unigenes containing SSRs were mostly associated with kenaf salt tolerance. Additionally, 10,483 SNVs were detected in contig sequences. Of the 3995 differentially expressed genes encoding interacting proteins, 1297 contained SSRs. Most of these genes were associated with metabolic pathways (e.g., 03000 transcription factors, B09132 signal transduction, and 04122 sulfur relay system). We designed 20 pairs of EST-SSR primers to genotype 30 kenaf varieties (lines), of which 9 primer pairs were ideal for genotyping (e.g., 1 highly polymorphic marker and 2 moderately polymorphic markers). The primer pairs designed for the EST-SSR markers in the kenaf genome may be useful SSR molecular markers for future research on kenaf. The verified polymorphic markers may be applicable to the molecular marker-assisted breeding of salt-tolerant kenaf varieties.
Assessing the Genetic Variability of Sweet Chestnut Varieties from the Tuscan Apennine Mountains (Italy)
Authors: Bini Lorenzo , Gori Massimo , Nin Stefania , Natale Roberto , Meacci Elisabetta , Giordani Edgardo , Biricolti Stefano
Published: July 2023

Castanea sativa Mill. is a valuable species with historical and economic importance in Europe, particularly in the Mediterranean area. In Italy, chestnut cultivation has been developed for centuries, leading to the recognition of more than 300 varieties. Nevertheless, a profusion of local names has been assigned by growers, causing the occurrence of synonyms and homonyms across the country. This research focused on genetic characterization and identification using 21 single sequence repeats (SSRs) for four chestnut varieties (i.e., Pastinese, Nerattino, Carpinese, and Rossola) commonly used for flour production in the Tuscan Apennine Mountains (Pistoia Province). A comprehensive number of 55 accessions identified by local growers as belonging to the four varieties were analyzed, in addition to a few “Marrone” accessions as outgroups. The 21 microsatellites were highly informative, detecting 98 alleles and displaying an average polymorphism information content (PIC) equal to 0.582. In addition, a considerable amount of genetic diversity was revealed, as shown by the heterozygosity levels (He = 0.634 and Ho = 0.475). The STRUCTURE analysis provided clear distinctions among the different varieties, splitting them into four separate groups. This result was also confirmed by UPGMA dendrogram and principal co-ordinates analysis (PCoA). However, one accession (Carp_5), previously identified as Carpinese, showed an allelic profile attributable to Pastinese, suggesting that farmers might have performed mislabeling or grafting propagation errors. Thus, our results confirm the use of SSRs to allocate the accessions of different varieties, uncovering possible synonyms and homonyms. Specifically, in the context of the Pistoiese mountain region, this tool can favor the traceability of processed products, such as flour, enhancing the quality and economic value of the local market. Lastly, our findings have revealed a considerable genetic variability within the Tuscan chestnut varieties whose preservation is mandatory to face climate change challenges through sustainable forest management practices.
Ethylene Regulates Combined Drought and Low Nitrogen Tolerance in Wheat: Proteomic Analysis
Authors: Yan Jiji , Wang Daoping , Kang Shuyu , He Zhang , Li Xin , Tang Wensi , Chen Kai , Pan Yinghong , Zhou Yongbin , Xu Zhaoshi , Chen Jun , Ma Youzhi , Chen Ming , Pang Chunhua
Published: July 2023

Wheat is a staple crop in China’s arid and semi-arid regions. Drought and low nitrogen (LN) are two major constraints to wheat growth and production. However, the molecular mechanism underlying wheat response to both drought and LN stress remains unknown. Accordingly, we conducted a proteomic study on the roots of two wheat varieties, Chang6878 (drought tolerant) and Shi4185 (drought sensitive) and compared the differences between drought and combined drought and LN stress treatments. In total, 5143 proteins were identified, of which 163 differentially abundant proteins (DAPs) were uniquely upregulated under drought and LN stress in Chang6878. Enrichment analysis showed that DAPs were mainly involved in mitogen-activated protein kinase signaling, phenylpropanoid biosynthesis, glutathione metabolism, ethylene biosynthesis, ethylene signal transduction, and oxidation–reduction reactions. These DAPs were verified via parallel reaction monitoring and quantitative real-time polymerase chain reaction. Chang6878 was treated with the ethylene synthesis precursor 1-aminocyclopropanecarboxylic acid, and its resistance to drought and LN stress improved. After treatment with the ethylene synthesis inhibitor silver nitrate and ethylene signal transduction inhibitor 1-methylcyclopropene, drought and LN stress resistance reduced. These results provide novel insights into the tolerance mechanisms of Chang6878 to drought and LN stress by altering ethylene synthesis and signal transduction. This study provides a reference for breeding drought- and low-nitrogen-tolerant wheat germplasm resources and a theoretical basis for maintaining food security in arid, barren areas.
Piceatannol as an Antiviral Inhibitor of PRV Infection In Vitro and In Vivo
Authors: Wang Zhiying , Cai Xiaojing , Ren Zhiyuan , Shao Yi , Xu Yongkang , Fu Lian , Zhu Yan
Published: July 2023

Pseudorabies virus (PRV) belongs to the family Herpesviridae. PRV has a wide host range and can cause cytopathic effects (CPEs) in PK-15 cells. Therefore, PRV was used as a model to study the antiviral activity of piceatannol. The results showed that piceatannol could restrain PRV multiplication in PK-15 cells in a dose-dependent manner. The 50% inhibitory concentration (IC50) was 0.0307 mg/mL, and the selectivity index (SI, CC50/IC50) was 3.68. Piceatannol could exert an anti-PRV effect by reducing the transcription level of viral genes, inhibiting PRV-induced apoptosis and elevating the levels of IL-4, TNF-α and IFN-γ in the serum of mice. Animal experiments showed that piceatannol could delay the onset of disease, reduce the viral load in the brain and kidney and reduce the pathological changes in the tissues and organs of the mice to improve the survival rate of the mice (14.3%). Therefore, the anti-PRV activity of piceatannol in vivo and in vitro was systematically evaluated in this study to provide scientific data for developing a new alternative measure for controlling PRV infection.
Gross Anatomy of the Female Reproductive System of Sugar Gliders (Petaurus breviceps)
Authors: Yllera María del Mar , Alonso-Peñarando Diana , Lombardero Matilde
Published: July 2023

We dissected carcasses of eight mature females, both parous and non-parous specimens, to study the macroscopic anatomy of the female reproductive system in the sugar glider. The genital system includes double organs, namely the right and left ones, which are completely separated. It includes two ovaries, two oviducts, two uteri and a vaginal complex. The uteri are fusiform-shaped and lack horns. The vaginal complex includes two lateral vaginae and a median vagina, also called the ‘birth canal’. The cranial end of both lateral vaginae partially fuses, forming an expansion named the vaginal sinus, which is divided into two parts by a longitudinal septum, one for each vagina, where the ipsilateral uterine cervix opens. The caudal end of the lateral vaginae opens into a medial and impar duct: the urogenital sinus that serves as a common passage for the reproductive and urinary systems. In non-pregnant females, only the lateral vaginae are present. In pregnant and recently parous females, a short median vagina extends from the caudal wall of the vaginal sinus to the cranial end of the urogenital sinus. In the ventral wall of this sinus, next to its caudal opening, there is a forked clitoris.
Comparison of Nonlinear Growth Models to Estimate Growth Curves in Kivircik Sheep under a Semi-Intensive Production System
Authors: Ozturk Nursen , Kecici Pembe Dilara , Serva Lorenzo , Ekiz Bulent , Magrin Luisa
Published: July 2023

The Kivircik is an indigenous sheep breed from Turkey, and it has superior meat quality compared to other indigenous breeds. Therefore, farmers prioritize Kivircik lamb fattening instead of milk production. Here, we aimed to determine the best nonlinear growth model, i.e., Gompertz, Logistic, Von Bertalanffy, and Brody, to describe the growth curve of Kivircik lambs. The body weight data from birth until 150 days of age belonging to 612 lambs were used as the material of this study. The best fitting model was selected by considering the adjusted coefficient of determination (R2adj), residual mean square, and Akaike’s (AIC) and Bayesian information criteria (BIC). Even though the Brody model had a better statistical fit, considering its biological interpretation, the Gompertz model was identified as an appropriate model for describing Kivircik lamb growth. Male lambs, twin lambs, and lambs born in winter had higher mature live weights (44.2 kg, 71.2 kg, and 38.5 kg, respectively) and rate of weight gain (2.1, 2.6, and 2.0, respectively). However, our subgroups revealed a similar rate of maturity (0.01). Growth models are important tools for deciding the optimal slaughter age and they provide valuable information on the management practices of both sexes, birth types, and birth seasons. These results can be applied to breeding programs for early selection, enabling intervention strategies when needed.
Melatonin Administration to Pregnant Ewes for Coccidiosis Control in Their Offspring
Authors: Bouroutzika Efterpi , Ciliberti Maria Giovanna , Caroprese Mariangela , Kantzoura Vaia , Theodosiadou Ekaterini K. , Batikas Georgios , Michailidis Marios-Lazaros , Stampinas Evaggelos-Georgios , Mimikou Zafeiro , Pantsios Georgios , Saratsis Anastasios , Valasi Irene
Published: July 2023

In livestock research, there has been a growing interest in the impact of melatonin on both health and disease conditions. The hypothesis of the present study was that melatonin treatment prenatally could support the immune competence and growth of experimentally infected lambs. This is the first study that aimed to investigate the impact of melatonin administration throughout pregnancy on immunity and oocyst excretion of pre-partum ewes and their offspring after experimental infection with Eimeria species. Thirty pregnant ewes were allocated into five equal groups, ΚΜ, ΚC, CM, CC, and NC, and gave birth to 47 lambs. Ewes of the KM and KC groups were orally challenged with a cocktail of Eimeria-sporulated oocysts (mainly consisting of Eimeria ovinoidalis), on day 120 of pregnancy, as well as all the lambs at the age of 5–9 days apart from those born from the NC group (environmental control). Fecal samples were collected from all ewes before infection and at parturition and from all lambs 14 times (S0–S13), before infection and during the following 8 weeks, for counting oocysts per gram of feces (OPG). Immunoglobulin (IgG) and cytokine (IL-1β, IL-6, IL-10, IFN-γ) levels were determined in ewes’ plasma collected before infection and at parturition, in lambs’ plasma at 24 and 72 h after their birth, and in colostrum samples at parturition and 72 h later. Body weight of lambs was recorded five times from birth until the age of 60 days. Accordingly, the leucogram was evaluated in blood samples collected six times within the same period. On average, IgG concentration was higher (p < 0.05) in the blood of KM-ewes compared to KC and CC groups and in colostrum of KM-ewes compared to other groups (p < 0.001). KM-lambs had greater IgG titer and IFN-γ level than the other groups (p < 0.05). The IL-10/ IFN-γ ratio in KM-ewes was lower than the CC group (p = 0.06). Overall, the growth rate of lambs did not differ among groups (p > 0.05). Total oocysts’ excretion in KM- and CM-lambs was reduced by 94.9% (p = 0.05) and 92.6% (p = 0.025), respectively, compared to KC-lambs, following the 3-week period after challenge, when E. ovinoidalis predominated in all groups. The dominant type of leucocytes was monocytes in all experimentally infected lambs, but not in NC-lambs, while overall lymphocytes were lower in KC-lambs than in NC-lambs (p < 0.05). Considering that almost all young indoor-reared lambs are exposed to coccidia species during their early life, melatonin treatment prenatally could suggest an alternative management tool in alleviating infection pressure.
Characterization of the Caudal Ventral Tubercle in the Sixth Cervical Vertebra in Modern Equus ferus caballus
Authors: May-Davis Sharon , Dzingle Diane , Saber Elle , Blades Eckelbarger Pamela
Published: July 2023

This study examined the anomalous variations of the ventral process of C6 in modern E. ferus caballus. The aim was to provide an incremental grading protocol measuring the absence of the caudal ventral tubercle (CVT) in this ventral process. The findings revealed the most prevalent absent CVT (aCVT) was left unilateral (n = 35), with bilateral (n = 29) and right unilateral (n = 12). Grading was determined in equal increments of absence 1/4, 2/4, 3/4, with 4/4 representing a complete aCVT in 56/76, with a significance of p = 0.0013. This also applied to bilateral specimens. In those C6 osseous specimens displaying a 4/4 grade aCVT, 41/56 had a partial absence of the caudal aspect of the cranial ventral tubercle (CrVT). Here, grading absent CrVTs (aCrVT) followed similarly to aCVTs, though 4/4 was not observed. The significance between 4/4 grade aCVTs and the presentation of an aCrVT was left p = 0.00001 and right p = 0.00018. In bilateral specimens, C6 morphologically resembled C5, implying a homeotic transformation that limited the attachment sites for the cranial and thoracal longus colli muscle. This potentially diminishes function and caudal cervical stability. Therefore, it is recommended that further studies examine the morphological extent of this equine complex vertebral malformation (ECVM) as well as its interrelationships and genetic code/blueprint.
What Is Written on a Dog’s Face? Evaluating the Impact of Facial Phenotypes on Communication between Humans and Canines
Authors: Sexton Courtney L. , Buckley Colleen , Lieberfarb Jake , Subiaul Francys , Hecht Erin E. , Bradley Brenda J.
Published: July 2023

Facial phenotypes are significant in communication with conspecifics among social primates. Less is understood about the impact of such markers in heterospecific encounters. Through behavioral and physical phenotype analyses of domesticated dogs living in human households, this study aims to evaluate the potential impact of superficial facial markings on dogs’ production of human-directed facial expressions. That is, this study explores how facial markings, such as eyebrows, patches, and widow’s peaks, are related to expressivity toward humans. We used the Dog Facial Action Coding System (DogFACS) as an objective measure of expressivity, and we developed an original schematic for a standardized coding of facial patterns and coloration on a sample of more than 100 male and female dogs (N = 103), aged from 6 months to 12 years, representing eight breed groups. The present study found a statistically significant, though weak, correlation between expression rate and facial complexity, with dogs with plainer faces tending to be more expressive (r = −0.326, p ≤ 0.001). Interestingly, for adult dogs, human companions characterized dogs’ rates of facial expressivity with more accuracy for dogs with plainer faces. Especially relevant to interspecies communication and cooperation, within-subject analyses revealed that dogs’ muscle movements were distributed more evenly across their facial regions in a highly social test condition compared to conditions in which they received ambiguous cues from their owners. On the whole, this study provides an original evaluation of how facial features may impact communication in human–dog interactions.
Variations in the Physical Properties and Microbial Community of Dairy Cow Manure—Implications for Testing and Efficacy of Footbathing Products
Authors: Palmer Maeve A. , Garland Martin J. , Stewart Linda D. , Helyar Sarah J. , O’Connell Niamh E.
Published: July 2023

Footbaths containing disinfectants are used on dairy farms to reduce the spread of digital dermatitis; however, they commonly become contaminated with manure. This trial investigated the physical properties and microbial composition of dairy cow manure from two production systems and examined whether the source of manure impacted the efficacy of footbathing disinfectants. Manure was collected from eighteen dairy cows, nine housed and fed grass silage (HOUSED) and nine at pasture (PASTURE). The pH and dry matter content was determined, total DNA was extracted and the region v3-v4 of the 16s rRNA gene sequenced. The efficacy of formalin and two trial products (TP1: peracetic acid and hydrogen peroxide; TP2: chlorocresol and triamine) was evaluated when mixed with manure from the two production systems. Production system differences were found in manure dry matter content, bacterial microbiome and the efficacy of both trial footbathing products but not formalin. The properties of manure affected the results of laboratory testing and therefore have the potential to influence footbathing disinfectant efficacy when footbaths are contaminated with manure. Further research into the impact of organic contaminants on the efficacy of disinfectants could facilitate the development of improved testing programmes and disinfectant products.