bluuebunny/crossref_metadata_2025 huggingface dataset:
Reply to Mehrotra et al. Wastewater-Based Surveillance Does Not Belong in a Regulatory Framework Designed to Protect Waters That Receive Treated Wastewater. Comment on “Wright, T.; Adhikari, A. Utilizing a National Wastewater Monitoring Program to Address the U.S. Opioid Epidemic: A Focus on Metro Atlanta, Georgia. Int. J. Environ. Res. Public Health 2023, 20, 5282”
Authors: Wright Tamara , Adhikari Atin
Published: August 2023

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Disordered Eating and Eating Disorders in Pediatric Obesity: Assessment and Next Steps
Authors: Chaves Eileen , Jeffrey D. Thomas , Williams Dominique R.
Published: August 2023

While the exact prevalence of disordered eating in youth who are overweight and have obesity has not been determined, studies show that the odds of a young adult (18–24 years) with obesity engaging in disordered eating behaviors is 2.45 times more likely to occur than in young adults with Body Mass Indexes (BMI) in the normative range. The purpose of this review is to highlight the role that disordered eating and eating disorders may play in pediatric obesity and the importance of screening for these conditions. The ability to identify and assess disordered eating alters the course of treatment. Without an understanding of the intersection of obesity and disordered eating, medical providers may continue treatment-as-usual. Doing so may inadvertently contribute to internalized weight bias in patients with obesity and exacerbate their disordered eating symptoms and behaviors. In addition, understanding the spectrum of disordered eating in pediatric patients with obesity allows providers to tailor treatments, discuss food and physical activity differently, and know when to refer patients to eating-disorder-specific providers for continued treatment.
Rural Knowledge Transformation in Terms of Mercury Used in Artisanal Small-Scale Gold Mining (ASGM)—A Case Study in Gorontalo, Indonesia
Authors: Metaragakusuma Andi Patiware , Sakakibara Masayuki , Arifin Yayu Indriati , Pateda Sri Manovita , Jahja Mohamad
Published: August 2023

Gorontalo is reputed to be one of the best-quality gold producers in the Indonesian archipelago. Gold production has been largely achieved through the activities of artisanal small-scale gold mining (ASGM), which as part of its extraction process, primarily uses mercury—a substance known to cause negative impacts on health and the environment, leading also to numerous socio-economic issues. This research aims (1) to investigate the extent of rural knowledge regarding mercury and to determine whether a video that explains mercury and the problems that occur as a result of ASGM can significantly transform rural populations’ knowledge; (2) to inspect different factors separating the SR group (those who live in the same regency as the ASGM area) from the NR group (those who live in the neighboring regency/city of the ASGM area) and to find out whether said factors are statistically significant. The results show that the test subjects’ knowledge of mercury had increased after watching the video, and that their willingness to oppose ASGM activities is one of the significant factors within the two groups. Moreover, this paper briefly describes the follow-up activities of the SRIREP project (Co-creation of Sustainable Regional Innovation for Reducing Risk of High-impact Environmental Pollution) in encouraging rural communities to explore sustainable livelihoods as an alternative to ASGM.
The Effects of Early-Life Stress on Liver Transcriptomics and the Protective Role of EPA in a Mouse Model of Early-Life-Stress-Induced Adolescent Depression
Authors: Zhao Jinlan , Ye Lihong , Liu Zuyi , Wu Jiayi , Deng Di , An Lin , Bai Shasha , Yang Lei , Liu Binjie , Shi Yafei , Liu Zhongqiu , Zhang Rong
Published: August 2023

Early-life stress (ELS) was found to increase the risk of adolescent depression, and clinical evidence indicated that eicosapentaenoic acid (EPA) was decreased in patients with adolescent depression, but the underlying mechanisms are unclear. Here, we utilized an ELS model of maternal separation with early weaning to explore the protective role of EPA in adolescent depression. We found that that ELS induced depression-like behavior rather than anxiety-like behavior in adolescent mice. RNA-sequencing results showed that ELS changed the transcription pattern in the liver, including 863 upregulated genes and 971 downregulated genes, especially those related to the biosynthesis of unsaturated fatty acids metabolism in the liver. Moreover, ELS decreased the expression of the rate-limiting enzymes, fatty acid desaturases 1/2 (FADS1/2), involved in the biosynthesis of EPA in the liver. Additionally, ELS reduced the levels of EPA in the liver, serum, and hippocampus, and EPA administration improved depression-like behavior-induced by ELS. Our results provide transcriptomic evidence that ELS increases the risk of adolescent depression by reducing the synthesis of unsaturated fatty acids in the liver, especially EPA, and suggest that supplementation with EPA should be investigated as a potential treatment for adolescent depression.
Oil-in-Water Pickering Emulsions Stabilized with Nanostructured Biopolymers: A Venue for Templating Bacterial Cellulose
Authors: Calvo Víctor , Fuentes Laura , Berdejo Daniel , González-Domínguez José M. , Maser Wolfgang K. , Benito Ana M.
Published: August 2023

Pickering emulsions (PEs) differ from conventional emulsions in the use of solid colloidal particles as stabilizing agents instead of traditional amphiphilic molecules. Nanostructured biopolymers (NBs) emerge as a promising alternative for PE stabilization owing to their remarkable biocompatibility, abundant availability, and low cost. To explore this potential, a study is herein presented, in which cellulose nanocrystals (CNCs), both type I and type II allomorphs, and chitin nanocrystals (ChNCs) were used for stabilizing oil-in-water PEs prepared by the use of ultrasound. Sunflower oil was selected as the oil phase as it offers the advantages of being edible, renewable, and inexpensive. By utilizing ζ-potential, static light diffraction, and visual observations, we determined the optimal oil/water ratio for each type of NB to obtain stable emulsions after 14 days. The optimized PEs were used to form bacterial nanocellulose composites through emulsion templating. To our knowledge, this study represents a pioneering work in exploiting oil-in-water PEs for this approach. Additionally, it entails the first utilization of nonmercerized type II CNCs as stabilizers for PEs, while also establishing a direct comparison among the most relevant NBs. The resulting composites exhibited a unique morphology, composed of larger pores compared to standard bacterial nanocellulose aerogels. These findings highlight the notable potential of NBs as stabilizers for PEs and their ability to generate green nanocomposites with tailored properties.
Optimization of a Nucleophilic Two-Step Radiosynthesis of 6-O-(2-[18F]fluoroethyl)-6-O-desmethyl-diprenorphine ([18F]FE-DPN) for PET Imaging of Brain Opioid Receptors
Authors: Németh Enikő , Gyuricza Barbara , Forgács Viktória , Cumming Paul , Henriksen Gjermund , Marton János , Bauer Beate , Mikecz Pál , Fekete Anikó
Published: August 2023

We have established a method for nucleophilic one-pot, two-step radiosynthesis of the popular opioid receptor radioligand 6-O-(2-[18F]fluoroethyl)-6-O-desmethyl-diprenorphine ([18F]FE-DPN) from the novel precursor 6-O-(2-tosyloxyethyl)-6-O-desmethyl- 3-O-trityl-diprenorphine (TE-TDDPN), which we designate as the Henriksen precursor. We undertook an optimization of the synthesis conditions, aiming to enhance the accessibility of [18F]FE-DPN for positron emission tomography (PET) studies of μ-opioid receptors. Herein, we report an optimized direct nucleophilic 18F-fluorination and the deprotection conditions for a fully automated radiosynthesis of [18F]FE-DPN on a modified GE Tracerlab FX FE synthesis panel. Starting from 1–1.5 GBq of [18F]fluoride and applying an Oasis Max 1cc cartridge for fluorine-18 trapping with a reduced amount of K2CO3 (5.06 μmol K+ ion), [18F]FE-DPN ([18F]11) was produced with 44.5 ± 10.6 RCY (decay-corrected), high radiochemical purity (>99%), and a molar activity of 32.2 ± 11.8 GBq/μmol in 60–65 min.
Resveratrol and 2-Ethyl-6-Methyl-3-Hydroxypiridine N-Acetyl Cysteinate as Protecting Agents upon the Stress Exposure
Authors: Zhigacheva Irina V. , Rusina Irina F. , Krikunova Natalia I. , Goloschapov Aleksandr N. , Veprintsev Timur L. , Yablonskaya Olga I. , Trofimov Aleksei V.
Published: August 2023

The increased generation of reactive oxygen species (ROS) by mitochondria under stress conditions leads to lipid peroxidation (LPO) as a consequence of the ROS interactions with polyunsaturated fatty acids in the lipid bilayer of cell membranes, causing their damage. It was assumed that chemical preparations that reduce the excessive ROS generation by mitochondria should exhibit protecting properties under oxidative-stress conditions. In this context, the antioxidants resveratrol (RSV) and 2-ethyl-6-methyl-3-hydroxypyridine N-acetylcysteinate (NAC-3-HP) were examined as potential chemical protectors upon the exposure to stress, able to maintain the functional state of mitochondria.
Natural Coumarin Shows Toxicity to Spodoptera litura by Inhibiting Detoxification Enzymes and Glycometabolism
Authors: Xia Tao , Liu Yan , Lu Zhanjun , Yu Haizhong
Published: August 2023

Coumarin and its derivatives are plant-derived compounds that exhibit potent insecticidal properties. In this study, we found that natural coumarin significantly inhibited the growth and development of Spodoptera litura larvae through toxicological assay. By transcriptomic sequencing, 80 and 45 differentially expressed genes (DEGs) related to detoxification were identified from 0 to 24 h and 24 to 48 h in S. litura after coumarin treatment, respectively. Enzyme activity analysis showed that CYP450 and acetylcholinesterase (AChE) activities significantly decreased at 48 h after coumarin treatment, while glutathione S-transferases (GST) activity increased at 24 h. Silencing of SlCYP324A16 gene by RNA interference significantly increased S. litura larval mortality and decreased individual weight after treatment with coumarin. Additionally, the expression levels of DEGs involved in glycolysis and tricarboxylic acid (TCA) cycle were inhibited at 24 h after coumarin treatment, while their expression levels were upregulated at 48 h. Furthermore, metabonomics analysis identified 391 differential metabolites involved in purine metabolism, amino acid metabolism, and TCA cycle from 0 to 24 h after treated with coumarin and 352 differential metabolites associated with ATP-binding cassette (ABC) transporters and amino acid metabolism. These results provide an in-depth understanding of the toxicological mechanism of coumarin on S. litura.
Effects of Tofacitinib on Muscle Remodeling in Experimental Rheumatoid Sarcopenia
Authors: Bermejo-Álvarez Ismael , Pérez-Baos Sandra , Gratal Paula , Medina Juan Pablo , Largo Raquel , Herrero-Beaumont Gabriel , Mediero Aránzazu
Published: August 2023

Sarcopenia is a frequent comorbidity of rheumatoid arthritis (RA). Clinical trials have shown that JAK inhibitors (JAKi) produce an asymptomatic increase in serum creatine kinase (CK) in RA, suggesting an impact on muscle. We evaluated the effect of JAKi in muscle remodeling in an experimental RA model. Antigen-induced arthritis (experimental RA, e-RA) was performed in 14 rabbits. Seven rabbits received tofacitinib (TOFA, orally 10 mg/kg/day). Animals were euthanized one day after the last ovalbumin injection, and muscles were prepared for histology, RT-PCR, and WB. C-reactive protein (CRP) and Myostatin (MSTN) serum concentration were determined by ELISA. Creatine and creatine kinase (CK) were analyzed. An increase in body weight as well as tibialis anterior cross-sectional area and diameter was observed in e-RA+TOFA vs. e-RA. e-RA decreased type II fibers and increased the myonuclei number, with all reverted by TOFA. TOFA did not modify CRP levels, neither did MSTN. TOFA significantly reduced IL-6, atrogin-1, and MuRF-1 compared with e-RA. e-RA+TOFA showed higher CK and lower creatine levels compared with e-RA. No differences in PAX-7 were found, while TOFA prevented the increase in MyoD1 in e-RA. Our model reflects the features of rheumatoid sarcopenia in RA. JAKi increased muscle mass through attenuating IL-6/JAK/STAT activation, decreasing atrogenes, and restoring muscle differentiation markers. These data together with an increase in CK support the role of CK as a valuable marker of muscle gain following JAKi treatment.
Insect Biodiversity in a Prealpine Suburban Hilly Area in Italy
Authors: Lupi Daniela , Zanetti Adriano , Triberti Paolo , Facchini Sergio , Rigato Fabrizio , Jucker Costanza , Malabusini Serena , Savoldelli Sara , Cortesi Paolo , Loni Augusto
Published: August 2023

Human activities and habitat fragmentation are known to greatly influence biodiversity. The aim of this study was to update an entomological checklist of a prealpine area in Italy, and also to evaluate the influence of different habitats and the proximity to cities on the entomological fauna. This study included different areas of a local park in Northern Italy, covering about 4000 ha, and situated at altitudes between 190 and 960 m asl. The surveys were carried out between 2010 and 2013 using different monitoring techniques (pitfall traps, car mounted nets, light traps, direct catches on soil and vegetation, visual sampling, gall collection). Furthermore, to assess the effect of habitat and locality on the composition of epigeic beetles, pitfall traps were set and inspected from April to September. All captured specimens were classified to species level. A total of 409 species were recorded, belonging to 7 orders and 78 families. A total of 76.1% were represented by Coleoptera, 13% Lepidoptera, 9.4% Hymenoptera, followed by other orders. In particular, some species with peculiar characteristics, or whose presence in the area had not been previously reported, were detected, such as Atheta pseudoelongatula, Ocypus rhaeticus, Tasgius tricinctus, Euplagia quadripunctaria, Scotopteryx angularia, Elachista constitella, Parornix bifurca, Oegoconia huemeri, and Lasius (Lasius) alienus. It seems possible that the habitat affected the community more than the locality. The woods showed a reduced biodiversity, and a simplified community structure. The comparison of the same habitats in different localities did not show significant differences.
Deep Autoencoder-Based Integrated Model for Anomaly Detection and Efficient Feature Extraction in IoT Networks
Authors: Alaghbari Khaled A. , Lim Heng-Siong , Saad Mohamad Hanif Md , Yong Yik Seng
Published: August 2023

The intrusion detection system (IDS) is a promising technology for ensuring security against cyber-attacks in internet-of-things networks. In conventional IDS, anomaly detection and feature extraction are performed by two different models. In this paper, we propose a new integrated model based on deep autoencoder (AE) for anomaly detection and feature extraction. Firstly, AE is trained based on normal network traffic and used later to detect anomalies. Then, the trained AE model is employed again to extract useful low-dimensional features for anomalous data without the need for a feature extraction training stage, which is required by other methods such as principal components analysis (PCA) and linear discriminant analysis (LDA). After that, the extracted features are used by a machine learning (ML) or deep learning (DL) classifier to determine the type of attack (multi-classification). The performance of the proposed unified approach was evaluated on real IoT datasets called N-BaIoT and MQTTset, which contain normal and malicious network traffics. The proposed AE was compared with other popular anomaly detection techniques such as one-class support vector machine (OC-SVM) and isolation forest (iForest), in terms of performance metrics (accuracy, precision, recall, and F1-score), and execution time. AE was found to identify attacks better than OC-SVM and iForest with fast detection time. The proposed feature extraction method aims to reduce the computation complexity while maintaining the performance metrics of the multi-classifier models as much as possible compared to their counterparts. We tested the model with different ML/DL classifiers such as decision tree, random forest, deep neural network (DNN), conventional neural network (CNN), and hybrid CNN with long short-term memory (LSTM). The experiment results showed the capability of the proposed model to simultaneously detect anomalous events and reduce the dimensionality of the data.
Recognition of Supraventricular Arrhythmias in Holter ECG Recordings by ECHOView Color Map: A Case Series Study
Authors: Naydenov Stefan , Jekova Irena , Krasteva Vessela
Published: August 2023

Ambulatory 24–72 h Holter ECG monitoring is recommended for patients with suspected arrhythmias, which are often transitory and might remain unseen in resting standard 12-lead ECG. Holter manufacturers provide software diagnostic tools to assist clinicians in evaluating these large amounts of data. Nevertheless, the identification of short arrhythmia events and differentiation of the arrhythmia type might be a problem in limited Holter ECG leads. This observational clinical study aims to explore a novel and weakly investigated ECG modality integrated into a commercial diagnostic tool ECHOView (medilog DARWIN 2, Schiller AG, Switzerland), while used for the interpretation of long-term Holter-ECG records by a cardiologist. The ECHOView transformation maps the beat waveform amplitude to a color-coded bar. One ECHOView page integrates stacked color bars of about 1740 sequential beats aligned by R-peak in a window (R ± 750 ms). The collected 3-lead Holter ECG recordings from 86 patients had a valid duration of 21 h 20 min (19 h 30 min–22 h 45 min), median (quartile range). The ECG rhythm was reviewed with 3491 (3192–3723) standard-grid ECG pages and a substantially few number of 51 (44–59) ECHOView pages that validated the ECHOView compression ratio of 67 (59–74) times. Comments on the ECG rhythm and ECHOView characteristic patterns are provided for 14 examples representative of the most common rhythm disorders seen in our population, including supraventricular arrhythmias (supraventricular extrasystoles, paroxysmal supraventricular arrhythmia, sinus tachycardia, supraventricular tachycardia, atrial fibrillation, and flutter) and ventricular arrhythmias (ventricular extrasystoles, non-sustained ventricular tachycardia). In summary, the ECHOView color map transforms the ECG modality into a novel diagnostic image of the patient’s rhythm that is comprehensively interpreted by a cardiologist. ECHOView has the potential to facilitate the manual overview of Holter ECG recordings, to visually identify short-term arrhythmia episodes, and to refine the diagnosis, especially in high-rate arrhythmias.
Protocol Study for the Evaluation of Non-Opioid Balanced General Anaesthesia in Cardiac Surgery with Cardiopulmonary Bypass: A Randomised, Controlled, Multicentric Superiority Trial (OFACAR Study)
Authors: Guinot Pierre-Grégoire , Besch Guillaume , Hameury Bastien , Grelet Tommy , Mertes Paul Michel , Nguyen Maxime , Bouhemad Belaid ,   on behalf of the OFACAR Study Group
Published: August 2023

Opioid-free anaesthesia (OFA) is general anaesthesia based on the use of several non-opioid molecules that aim to have an analgesic effect, decrease the sympathetic response, decrease hormonal stress, and decrease the inflammatory response during surgery. Although this approach to anaesthesia is regularly used in clinical practice, it remains a novel approach. The literature on this anaesthesia modality finds a number of positive effects on cardiac, respiratory, and cognitive function but no randomised study evaluated these effects during cardiac surgery where there is a high incidence of postoperative complications. The main aim of the study is to compare OFA vs. standard balanced opioid general anaesthesia on the incidence of postoperative complications and the length of stay in intensive care and hospital. OFACAR is a multicentric, randomised, controlled, superiority, single-blind, two parallel-arm clinical trial in patients undergoing cardiac surgery with cardiopulmonary bypass. We compared a balanced general anaesthesia without opioids (OFA group) vs. a balanced opioid general anaesthesia with sufentanil (control group). One hundred and sixty patients will be enrolled in each treatment group. The primary endpoint is a composite one which corresponds to the occurrence of at least one of the postoperative complications, defined according to European standards within 30 days after surgery. Recruitment started in September 2019, and data collection is expected to end in November 2024.
Characterization of Immunogenicity and Safety of COVID-19 mRNA-1273 in HIV-Positive Italian Patients with Hemophilia: A Prospective Single-Center Cohort Study
Authors: Suffritti Chiara , Gualtierotti Roberta , Arcudi Sara , Ciavarella Alessandro , Novembrino Cristina , Lecchi Anna , La Marca Silvia , Padovan Lidia , Scalambrino Erica , Clerici Marigrazia , Bono Patrizia , Ceriotti Ferruccio , Muscatello Antonio , Siboni Simona Maria , Peyvandi Flora
Published: August 2023

To characterize the immunogenicity of mRNA-1273 (Moderna, Cambridge, MA, USA) vaccine in HIV-positive hemophilic patients during the third COVID-19 wave in Italy and to investigate biomarkers of coagulation and endothelial perturbation before and after complete vaccination schedule, twenty-three consecutive adult HIV-positive patients with hemophilia were included. Blood was collected before and two weeks after vaccination. We measured anti-SARS-CoV-2 spike protein antibodies to assess immunogenicity; circulating biomarkers of coagulation (protein C and D-dimer), endothelial perturbation (von Willebrand factor (VWF)) and anti-Platelet Factor 4 (PF4) antibodies were analyzed. Flow-based analysis of thrombus formation was performed in nine patients using a flow-chamber device. Two weeks after completing the vaccination schedule, all patients had anti-spike antibodies values consistent with an effective immunization. Mean (±standard deviation) basal values of protein C and VWF (106 ± 21% and 171 ± 45%, respectively) were not significantly different from data obtained two weeks after the second dose (103 ± 20%, 162 ± 43%, respectively). D-dimer median values (interquartile range) were not significantly different at baseline (442 (603–142) ng/mL) and after the second dose (477 (654–262) ng/mL). Anti-PF4 antibodies were detected in three patients with no associated clinical manifestations. No significant differences were found in flow-based analysis of thrombus formation. Our data demonstrate that in HIV-positive patients with hemophilia, SARS-CoV-2 vaccination is effective and safe, with no effects on coagulation and endothelial perturbation.
A Machine Learning Model for the Accurate Prediction of 1-Year Survival in TAVI Patients: A Retrospective Observational Cohort Study
Authors: Pollari Francesco , Hitzl Wolfgang , Rottmann Magnus , Vogt Ferdinand , Ledwon Miroslaw , Langhammer Christian , Eckner Dennis , Jessl Jürgen , Bertsch Thomas , Pauschinger Matthias , Fischlein Theodor
Published: August 2023

Background: predicting the 1-year survival of patients undergoing transcatheter aortic valve implantation (TAVI) is indispensable for managing safe early discharge strategies and resource optimization. Methods: Routinely acquired data (134 variables) were used from 629 patients, who underwent transfemoral TAVI from 2012 up to 2018. Support vector machines, neuronal networks, random forests, nearest neighbour and Bayes models were used with new, previously unseen patients to predict 1-year mortality in TAVI patients. A genetic variable selection algorithm identified a set of predictor variables with high predictive power. Results: Univariate analyses revealed 19 variables (clinical, laboratory, echocardiographic, computed tomographic and ECG) that significantly influence 1-year survival. Before applying the reject option, the model performances in terms of negative predictive value (NPV) and positive predictive value (PPV) were similar between all models. After applying the reject option, the random forest model identified a subcohort showing a negative predictive value of 96% (positive predictive value = 92%, accuracy = 96%). Conclusions: Our model can predict the 1-year survival with very high negative and sufficiently high positive predictive value, with very high accuracy. The “reject option” allows a high performance and harmonic integration of machine learning in the clinical decision process.
Agreement between Five Experts and the Laguna ONhE Automatic Colourimetric Application Interpreting the Glaucomatous Aspect of the Optic Nerve
Authors: Mendez-Hernandez Carmen , Gutierrez-Diaz Esperanza , Pazos Marta , Gimenez-Gomez Rafael , Pinazo-Duran Maria Dolores
Published: August 2023

Background: Optic nerve head (ONH) interpretation is a glaucoma screening method which may be influenced by criteria variability. Laguna ONhE software is a low-cost and non-invasive method of ONH analysis. Methods: We analysed the results of the Laguna ONhE application, interpreting 552 ONH images from the ACRIMA database, publicly available on the Internet, and compared them with the opinion of five experts. Diagnostic agreement was investigated using Cohen’s kappa (κ) with 95% confidence. Results: The kappa concordance index obtained with Laguna ONhE and the majority of the experts’ criterion (0.77) was significantly higher compared to that obtained with ACRIMA and the majority of the experts’ criterion (0.61). In 44.7% of the cases there was absolute agreement among the 5 experts and the Laguna ONhE program. Removing borderline cases from the analysis yielded increased diagnostic agreement (0.81). The area under the receiver operating characteristic (AUROC) of the Laguna ONhE program (0.953, p < 0.001) was not significantly different than AUROC of the majority of the experts’ criterion (0.925, p < 0.001), p = 0.052. Individually obtained expert’s AUROCs were significantly lower (0.636 to 0.913; p < 0.01). Conclusions: Laguna ONhE’s agreement with the experts is high, particularly where the diagnosis may be more obvious by the appearance of the ONH.
Evaluating the Efficacy of Tension Band Wiring Fixation for Chaput Tubercle Fractures
Authors: Yoon Sung-Joon , Yeo Eui-Dong , Jung Ki-Jin , Hong Yong-Cheol , Hong Chang-Hwa , Won Sung-Hun , Lee Kyung-Jin , Ji Jae-Young , Byeon Je-Yeon , Lee Dhong-Won , Kim Woo-Jong
Published: August 2023

Background: Chaput tubercle fractures, located at the attachment site of the anterior inferior tibiofibular ligament (AITFL) on the distal tibia, have the potential to destabilize the syndesmosis joint. This study aims to assess the effectiveness of tension band wiring (TBW) as a surgical intervention for managing Chaput fractures and the consequent syndesmosis instability. Methods: A retrospective review of patient charts was undertaken for those who had undergone ankle fracture surgery from April 2019 through May 2022. The surgical procedure involved direct fixation of the Chaput fractures using the TBW method. Radiological assessments were performed using postoperative simple radiographs and computed tomography (CT) scans, while clinical outcomes were evaluated using the Olerud–Molander Ankle Score (OMAS) and the visual analog scale (VAS). Results: The study included 21 patients. The average OMAS improved significantly, rising from 5.95 preoperatively to 83.57 postoperatively. Similarly, the average VAS score dropped from 7.95 before the surgery to 0.19 thereafter. Minor wound complications were reported by three patients, and one case of superficial infection was resolved with antibiotic therapy. Conclusions: Our findings suggest that the TBW technique is an effective surgical approach for treating Chaput fractures and associated syndesmosis instability. It provides reliable fixation strength and leads to improved long-term functional outcomes. Further research is needed to compare the TBW technique with alternative methods and optimize the treatment strategies for these complex ankle fractures.
Pregnant Women at Low Risk of Having a Child with Fetal and Neonatal Alloimmune Thrombocytopenia Do Not Require Treatment with Intravenous Immunoglobulin
Authors: Kjeldsen-Kragh Jens , Bein Gregor , Tiller Heidi
Published: August 2023

Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a rare condition in which maternal alloantibodies to fetal platelets cause fetal thrombocytopenia that may lead to intracranial hemorrhage (ICH). Off-label intravenous immunoglobulin (IVIg) has for 30 years been the standard of care for pregnant women who previously have had a child with FNAIT. The efficacy of this treatment has never been tested in a placebo-controlled clinical trial. Although IVIg treatment may improve the neonatal outcome in women who previously have had a child with FNAIT-associated ICH, the question is whether IVIg is necessary for all immunized pregnant women at risk of having a child with FNAIT. The results from some recent publications suggest that antenatal IVIg treatment is not necessary for women who are (1) HPA-1a-immunized and HLA-DRB3*01:01-negative, (2) HPA-1a-immunized with a previous child with FNAIT but without ICH or (3) HPA-5b-immunized. If IVIg is not used for these categories of pregnant women, the amount of IVIg used in pregnant women with platelet antibodies would be reduced to less than ¼ of today’s use. This is important because IVIg is a scarce resource, and the collection of plasma for the treatment of one pregnant woman is not only extremely expensive but also requires tremendous donor efforts.
Triple Semi-Circular Canal Occlusion and Cochlear Implantation: A Treatment Option for Single-Sided Menière’s Disease with Functional Deafness—A Case Series
Authors: Jacob Ronny , Lauer Gina , Ernst Arneborg , Seidl Rainer , Kiefer Lenneke , Mittmann Philipp
Published: August 2023

The surgical options for patients with single-sided Menière’s disease and functional deafness are challenging. Our case series reports the outcomes of surgical treatments of patients with single-sided Menière’s disease and functional deafness. These patients have undergone a one-staged occlusion of all semi-circular canals and cochlear implantation. Five patients (four female and one male; 62 ± 8.2 years with a range from 50 to 72 years) with single-sided Menière’s disease and functional deafness were included in this study. In all cases, the patients suffered from frequent rotational vertigo episodes for many years. Other treatment options (e.g., medication) had not yet been successful. Preoperatively, the Dizziness Handicap Inventory (DHI) of all patients indicated severe emotional, physical, and functional deficits. Patients showed a functional (near-total) deafness of the affected ear in all cases. All patients were supplied with cochlear implants in combination with a triple occlusion of all semi-circular canals in a one-stage procedure. After a short period of increased dizziness following surgery and after the activation of the cochlear implant and CI rehabilitation (auditory-verbal therapy), vertigo control and an adequate audiological outcome were achieved. The DHI showed a constant decrease after surgery. The combination of a triple semi-circular canal occlusion and cochlear implantation can be an efficient treatment for patients with single-sided Menière’s disease.
Unique Characteristics of Women and Infants Moderate the Association between Depression and Mother–Infant Interaction
Authors: Weiss Sandra J. , Goodman Sherryl H. , Kidd Sharon A. , Owen Margaret Tresch , Simeonova Diana I. , Kim Christine Youngwon , Cooper Bruce , Rosenblum Katherine L. , Muzik Maria
Published: August 2023

Research has shown mixed results regarding the association between women’s postpartum depression and mother–infant interactions, suggesting that a woman’s unique experience and context may moderate how depression shapes these interactions. We examined the extent to which a woman’s comorbid anxiety, her exposure to adversity, and infant characteristics moderate the relationship between depressive symptoms of women and interactions with their infants at 6 (n = 647) and 12 months (n = 346) postpartum. The methods included standardized coding of mother–infant interactions and structural regression modeling. The results at 6 months of infant age indicated that infant male sex and infant negative affectivity were risk factors for mothers’ depression being associated with less optimal interactions. At 12 months of infant age, two moderators appeared to buffer the influence of depression: a woman’s history of trauma and infant preterm birth (≤37 weeks gestation). The results reinforce the salience of infant characteristics in the relationship between maternal depression and mother–infant interactions. The findings also suggest that experiences of trauma may offer opportunities for psychological growth that foster constructive management of depression’s potential effect on mother–infant interactions. Further research is needed to clarify the underlying processes and mechanisms that explain the influence of these moderators. The ultimate goals are to reduce the risk of suboptimal interactions and reinforce healthy dyadic relations.
Innovative Smart Drilling with Critical Event Detection and Material Classification
Authors: Chaiprabha Kantawatchr , Chancharoen Ratchatin
Published: August 2023

This work presents a cyber-physical drilling machine that incorporates technologies discovered in the fourth industrial revolution. The machine is designed to realize its state by detecting whether it hits or breaks through the workpiece, without the need for additional sensors apart from the position sensor. Such self-recognition enables the machine to adapt and shift the controllers that handle position, velocity, and force, based on the workpiece and the drilling environment. In the experiment, the machine can detect and switch controls that follow the drilling events (HIT and BREAKHTROUGH) within 0.1 and 0.5 s, respectively. The machine’s high visibility design is beneficial for classification of the workpiece material. By using a support-vector-machine (SVM) on thrust force and feed rate, the authors are seen to achieve 92.86% accuracy for classification of material, such as medium-density fiberboard (MDF), acrylic, and glass.
Multi-Objective Parametric Shape Optimisation of Body-Centred Cubic Lattice Structures for Additive Manufacturing
Authors: Ali Hafiz Muhammad Asad , Abdi Meisam
Published: August 2023

There has been significant interest in additively manufactured lattice structures in recent years due to their enhanced mechanical and multi-physics properties, making them suitable candidates for various applications. This study presents a multi-parameter implicit equation model for designing body-centred cubic (BCC) lattice structures. The model is used in conjunction with a multi-objective genetic algorithm (MOGA) approach to maximise the stiffness of the BCC lattice structure while minimising von-Mises stress within the structure under a specific loading condition. The selected design from the MOGA at a specific lattice density is compared with the classical BCC lattice structure and the designs generated by a single-objective genetic algorithm, which focuses on maximising stiffness or minimising von-Mises stress alone. By conducting a finite element analysis on the optimised samples and performing mechanical testing on the corresponding 3D-printed specimens, it was observed that the optimised lattice structures exhibited a substantial improvement in mechanical performance compared to the classical BCC model. The suitability of multi-objective and single-objective optimisation approaches for designing lattice structures was further investigated by comparing the corresponding designs in terms of their stiffness and maximum von-Mises stress values. The results from the numerical analysis and experimental testing demonstrate the significance of the application of an appropriate optimisation strategy for designing lattice structures for additive manufacturing.
Radical Hysterectomy in Early-Stage Cervical Cancer: Abandoning the One-Fits-All Concept
Authors: Bianchi Tommaso , Grassi Tommaso , Bazzurini Luca , Di Martino Giampaolo , Negri Serena , Fruscio Robert , Trezzi Gaetano , Landoni Fabio
Published: August 2023

Two pillars in modern oncology are treatment personalization and the reduction in treatment-related morbidity. For decades, the one-fits-all concept of radical hysterectomy has been the cornerstone of early-stage cervical cancer surgical treatment. However, no agreement exists about the prevalent method of parametrial invasion, and the literature is conflicting regarding the extent of parametrectomy needed to achieve adequate surgical radicality. Therefore, authors started investigating if less radical surgery was feasible and oncologically safe in these patients. Two historical randomized controlled trials (RCTs) compared classical radical hysterectomy (RH) to modified RH and simple hysterectomy. Less radical surgery showed a drastic reduction in morbidity without jeopardizing oncological outcomes. However, given the high frequency of adjuvant radiotherapy, the real impact of reduced radicality could not be estimated. Subsequently, several retrospective studies investigated the chance of tailoring parametrectomy according to the tumor’s characteristics. Parametrial involvement was shown to be negligible in early-stage low-risk cervical cancer. An observational prospective study and a phase II exploratory RCT have recently confirmed the feasibility and safety of simple hysterectomy in this subgroup of patients. The preliminary results of a large prospective RCT comparing simple vs. radical surgery for early-stage low-risk cervical cancer show strong probability of giving a final answer on this topic.
A Novel Integrated Spatiotemporal-Variable Model of Landscape Changes in Traditional Villages in the Jinshaan Gorge, Yellow River Basin
Authors: Liu Lili , Chen Meng , Luo Pingping , Hu Maochuan , Duan Weili , Elbeltagi Ahmed
Published: August 2023

Spatiotemporal studies of landscape pattern evolution in traditional villages are beneficial for addressing complex urbanization and global climate change. Using the traditional villages of Jiaxian and Linxian in the Jinshaan Gorge of the Yellow River Basin, this study employed a three-dimensional (3D) analysis involving three spatial scales (macro, meso, and micro), temporal scales (past, present, and future), and variables (humanity, society, and nature) based on the methods of spatiotemporal data analysis (SDA), geographic information system, remote sensing, and landscape pattern index (LPI) by Fragstats. On the macro scale, a significant turning point in ecological conservation awareness was indicated by LPI and SDA. Urban and rural construction land continuously increased because of urbanization. Plowland, grassland, and woodland were the main influencing factors in the evolution of rural settlements, with a 0.42% cumulative transformation rate. On the meso scale, the interactions and mutual promotion of mountain and aquatic environments, aquatic facilities, agricultural production, and cultural heritage have shaped the socioeconomic dimensions of evolution. On the micro scale, with urbanization, some traditional humanistic spaces have lost their original functions. A novel spatiotemporal-variable quantitative model explored the spatiotemporal evolution characteristics of human–land coupling, which can be used for the sustainable development of river basins worldwide.
Clonal Hematopoiesis of Indeterminate Potential and Cardiovascular Risk in Patients with Chronic Kidney Disease without Previous Cardiac Pathology
Authors: Kislikova Maria , Lopez Maria Ana Batlle , Salinas Francisco Javier Freire , Blanco José Antonio Parra , Molina Maria Pilar García-Berbel , Fernandez Alejandro Aguilera , Haces Vicente Celestino Piñera , Unzueta Maria Teresa García , Hernández Adalberto Benito , Millan Juan Carlos Ruiz San , Rodrigo Calabia Emilio
Published: August 2023

Clonal hematopoiesis of indeterminate potential (CHIP) is defined by the clonal expansion of hematopoietic stem cells carrying certain genes associated with an increased risk of hematological malignancies. Our study analyzes the influence of CHIP on the risk of heart disease and cardiovascular events in a population with chronic kidney disease (CKD). A total of 128 patients were prospectively followed up for 18 months to detect major cardiovascular events (MACE). To detect the presence of silent heart disease, troponin I, NT-Pro-BNP, and coronary calcification were measured. A massive sequencing was performed to detect CHIP. A total of 24.2% of the patients presented CHIP, including that which was only pathogenic. The most frequently affected gene was TET2 (21.1%). Using multivariate logistic regression analysis, the presence of CHIP was not related to coronary calcification (OR 0.387, 95% CI 0.142–1.058, p = 0.387), nor was it related to troponin I or NT-Pro-BNP. A total of nine patients developed major cardiovascular events. Patients with CHIP did not have a higher risk of major cardiovascular events, although patients with DNMT3A did have a higher risk (HR 6.637, 95% CI 1.443–30.533, p = 0.015), independent of other variables. We did not find that CHIP was associated with a greater risk of silent heart disease or cardiovascular events, although those affected by DNMT3a, analyzed independently, were associated with a greater number of cardiovascular events.
Long-Term Changes in Retinal Nerve Fiber Layer Thickness after Vitrectomy for Epiretinal Membrane Using Optical Coherence Tomography Images
Authors: Bae Ki Woong , Kim Dong Ik , Hwang Daniel Duck-Jin
Published: August 2023

This study investigated the long-term effects of epiretinal membrane (ERM) surgery on peripapillary retinal nerve fiber layer (RNFL) thickness using optical coherence tomography (OCT) images. We included 30 patients with idiopathic ERM who underwent a vitrectomy for ERM removal with internal limiting membrane peeling. The patients were followed up for 5 years after surgery, and their medical records were reviewed for best-corrected visual acuity (BCVA) and OCT parameters. The study population comprised 24 females (80.0%), and the mean age was 65.4 ± 7.2 years. The baseline BCVA significantly improved from 0.28 ± 0.24 to 0.12 ± 0.09 logMAR (p < 0.001) 1 year after surgery and continued to improve for 5 years after surgery. The peripapillary RNFL thickness initially increased after surgery and then gradually decreased. The peripapillary RNFL thicknesses of the global and temporal sectors showed significant reductions 2 years after surgery, whereas those of the nasal sectors did not significantly change. The peripapillary RNFL thickness was thinner in the global and temporal areas of the operated eyes than in those of the fellow eyes 4 and 5 years after surgery. In conclusion, peripapillary RNFL thicknesses decreased in the global and temporal areas after ERM surgery, whereas peripapillary RNFL thicknesses in the nasal sectors did not change significantly during the long-term follow-up.
Synthesis of a Novel 2-((4,5-Diphenyl-4H-1,2,4-triazol-3-yl)thio)acetaldehyde as a Bisulfite Adduct
Authors: Pintea Beniamin-Nicolae , Burcă Ion , Badea Valentin , Peter Francisc
Published: August 2023

The scope of the current work was to synthesize an S-alkylated 1,2,4-triazole-3-thiol derivative. Synthesis was carried out in two steps: in the first step, 4,5-diphenyl-4H-1,2,4-triazole-3-thiol was S-alkylated using a halogenated acetal and cesium carbonate. In the second step, several acetal deprotection procedures were tested, and the aldehyde obtained was isolated as a bisulfite adduct. The structures of the new compounds were characterized by FT-IR, 1D, and 2D NMR spectroscopic methods.
On the Change in Hydrogen Diffusion and Trapping Behaviour of Pearlitic Rail Steel at Different Stages of Production
Authors: Eichinger Matthias , Loder Bernd , Tkadletz Michael , Schnideritsch Holger , Klösch Gerald , Mori Gregor
Published: August 2023

To avoid hydrogen flaking in rail production, it is of crucial importance to understand the differences in hydrogen diffusion and trapping between different production steps. Therefore, as-cast unfinished material was compared with two finished rails, hot-rolled and head-hardened, using electron backscattered diffraction (EBSD), electrochemical permeation, and thermal desorption spectroscopy (TDS). A significant increase in dislocation density was in the head-hardened rail compared with the other material states. This leads to an effective hydrogen diffusion coefficient of 5.8 × 10−7 cm2/s which is lower by a factor of four than the diffusion coefficients examined in the other states. Thermal desorption spectroscopy analyses show a clear difference between unfinished and finished rail materials. While a peak in activation energy between 32 and 38 kJ/mol is present at all states, only as-cast unfinished material shows a second peak with an activation energy of 47 kJ/mol, which is related to microvoids. The results show that in the investigated material, the effect of increasing dislocation density has a stronger influence on the effective diffusion coefficient than the presence of a second active trapping site.
Experimental vs. Theoretical Viscosity Determination of Aluminosilicate Glasses
Authors: Zawada Anna , Lubas Malgorzata , Nowak Adrian
Published: August 2023

The paper presents the results of studies on the viscosity of the glass mass in various temperature ranges, determining the basic technological parameter, very important from the point of view of melting and forming. For this purpose, six sets based on natural raw materials such as basalt, dolomite, and amphibolite, modified with different amounts of float glass cullet, were melted. The melting process was carried out in an electric furnace at the temperature of 1450 °C for 2 h. Using the dilatometric method, high-temperature microscopy and theoretical calculation methods, the viscosity of the produced glasses was determined in various temperature ranges. Comparative analyses of the employed methods were carried out. The significance of the applied calculation methods for aluminosilicate glasses depending on the basic chemical composition of the glasses was presented. The relationship between the manner of incorporating amphoteric ions Al3+, Fe3+ and Mg2+ into the glass structure and the change in viscosity in the temperature range corresponding to the working point range at 104 [dPa·s] viscosity and the relaxation range—Tg temperature at 1013 [dPa·s] viscosity was justified. It was justified that in order to plot the viscosity curve with the correct slope in the forming range for aluminosilicate glasses, it is appropriate to use the two-point method based on the fixed viscosity points of 104 [dPa·s] and 1013 [dPa·s].