bluuebunny/crossref_metadata_2025 huggingface dataset:
Wearable IoTs and Geo-Fencing Based Framework for COVID-19 Remote Patient Health Monitoring and Quarantine Management to Control the Pandemic
Authors: Ullah Farman , Haq Hassan Ul , Khan Jebran , Safeer Arslan Ali , Asif Usman , Lee Sungchang
Published: August 2021

The epidemic disease of Severe Acute Respiratory Syndrome (SARS) called COVID-19 has become a more frequently active disease. Managing and monitoring COVID-19 patients is still a challenging issue for advanced technologies. The first and foremost critical issue in COVID-19 is to diagnose it timely and cut off the chain of transmission by isolating the susceptible and patients. COVID-19 spreads through close interaction and contact with an infected person. It has affected the entire world, and every country is facing the challenges of having adequate medical facilities along with the availability of medical staff in rural and urban areas that have a high number of patients due to the pandemic. Due to the invasive method of treatment, SARS-COVID is spreading swiftly. In this paper, we propose an intelligent health monitoring framework using wearable Internet of Things (IoT) and Geo-fencing for COVID-19 susceptible and patient monitoring, and isolation and quarantine management to control the pandemic. The proposed system consists of four layers, and each layer has different functionality: a wearable sensors layer, IoT gateway layer, cloud server layer, and client application layer for visualization and analysis. The wearable sensors layer consists of wearable biomedical and GPS sensors for physiological parameters, and GPS and Wi-Fi Received Signal Strength Indicator acquisition for health monitoring and user Geo-fencing. The IoT gateway layer provides a Bluetooth and Wi-Fi based wireless body area network and IoT environment for data transmission anytime and anywhere. Cloud servers use Raspberry Pi and ThingSpeak cloud for data analysis and web-based application layers for remote monitoring based on user consent. The susceptible and patient conditions, real-time sensor’s data, and Geo-fencing enables minimizing the spread through close interaction. The results show the effectiveness of the proposed framework.
Analytical Study and Comparison of Electromagnetic Characteristics of 8-Pole 9-Slot and 8-Pole 12-Slot Permanent Magnet Synchronous Machines Considering Rotor Eccentricity
Authors: Lee Hoon-Ki , Bang Tae-Kyoung , Lee Jeong-In , Woo Jong-Hyeon , Shin Hyo-Seob , Yoon Ick-Jae , Choi Jang-Young
Published: August 2021

In this study, a magnetic field is analyzed using an analytical method and compared with the electromagnetic characteristics of 8-pole 9-slot and 8-pole 12-slot permanent magnet synchronous machines considering rotor eccentricity. The magnetic flux density and back electromotive force (EMF) are derived using perturbation theory and electromagnetic theory. First, the Fourier modeling of a permanent magnet is performed through magnetization modeling, and two analysis regions are set based on several assumptions for applying the analytical method. Accordingly, the governing equations of the analysis regions are derived in the form of Poisson and Laplace equations. In addition, the undefined coefficients of the general solutions are calculated through general solutions and appropriate boundary conditions, and the magnetic flux density and back EMF of the air gap region are derived based on the definition of the magnetic vector potential. The results obtained using the analytical method are compared with the finite element method and experimental results. In addition, we perform a torque analysis considering rotor eccentricity and analyze the torque ripple based on rotor eccentricity for two cases involving the pole/slot combination.
Numerical Investigation into the Effects of Design Parameters on the Flow Characteristics in a Turbine Exhaust Diffuser
Authors: Lee Byung Ju , Chung Jin Taek
Published: August 2021

In this study, we numerically investigated the effects of design parameters, such as the strut geometry or diffusion angle, on the performance of an industrial turbine exhaust diffuser. Turbine exhaust diffusers are commonly used to change the kinetic energy of exhaust gases from the outlet of turbine stages into the static pressure. The turbine exhaust diffuser investigated in this work consisted of an annular diffuser with five identical struts equally spaced around the front circumference and a conical diffuser with a hub extension at the rear. Four design parameters were considered and several values for each parameter were tested in this study. The aerodynamic performances of the studied diffusers were evaluated according to their pressure recovery coefficients and rates of total pressure loss. Contours for the velocity, pressure, and entropy increase were plotted and compared for the various diffuser shapes. The numerical results showed that the strut thickness and the axially swept angle of the strut significantly influence the aerodynamic performance of the turbine exhaust diffuser, whereas the strut lean angle and the diffuser hade angle are less important.
Implementation of a Resonant Converter with Topology Morphing to Achieve Bidirectional Power Flow
Authors: Lin Bor-Ren
Published: August 2021

A DC converter with the benefits of reverse power capability, less switching loss and wide voltage operation is presented and implemented for wide input voltage applications such as fuel cell energy, photovoltaic (PV) system and DC wind power. Two full bridge resonant circuits are used in the presented converter to achieve bidirectional power flow capability and reduce switching losses on active devices. To overcome the wide input DC voltage variation problem for fuel cell energy and PV solar panel, the topology morphing between the half bridge circuit and full bridge circuit is adopted on the primary side to obtain low (or high) voltage gain under a high (or low) input voltage condition. Therefore, the stable DC voltage is controlled at the load side by using the variable switching frequency modulation. The studied hybrid CLLC converter is tested by a 1 kW prototype and the performance is verified and confirmed by experiments.
Cryopreservation and Micropropagation Methods for Conservation of Genetic Resources of Ulmus laevis and Ulmus glabra
Authors: Välimäki Sakari , Rusanen Mari , Pečínková Daniela , Tikkinen Mikko , Aronen Tuija
Published: August 2021

Elms are threatened by Dutch elm disease, and conservation methods are needed to protect their genetic diversity. Cryopreservation of dormant buds allows large numbers of genotypes to be conserved with small space requirements and minimal upkeep. Cryopreservation through slow controlled cooling was tested for both elm species native to Finland, Ulmus glabra and Ulmus laevis. Regeneration of the thawed buds by micropropagation was studied on different basal media and using different growth regulators. Multiple surface sterilisation methods were tried out for bud explants. The multiplication of U. glabra was investigated with Driver and Kuniyuki walnut medium with either 0.5 mg/L meta-topolin or 0.5 mg/L 6-benzylaminopurine. Rooting with short indole-6-butyric acid induction in liquid medium and direct transplantation of the shoots to peat ex vitro after induction were tested. For initiation, either Murashige and Skoog or Driver and Kuniyuki walnut medium with 0.02 mg/L gibberellic acid 4 + 7 and 0.5 mg/L 6-benzylaminopurine were found to best promote shoot formation. Surface sterilisation remains the most challenging step. No significant differences were found between the multiplication media in either shoot production or rooting success. Rooting by direct transplanting was achieved in both species, but further development is required before application on a larger scale. With further improvements to sterilisation success especially in U. glabra, the method can be applied to the conservation of genetic resources of both U. laevis and U. glabra, and knowledge of regeneration success can be used to design the cryoconservation plan and optimise the sampling.
Predicting Suitable Environments and Potential Occurrences for Cinnamomum camphora (Linn.) Presl.
Authors: Meng Jiasong , Li Miao , Guo Jinhui , Zhao Daqiu , Tao Jun
Published: August 2021

Global climate change has created a major threat to biodiversity. However, little is known about the habitat and distribution characteristics of Cinnamomum camphora (Linn.) Presl., an evergreen tree growing in tropical and subtropical Asia, as well as the factors influencing its distribution. The present study employed Maxent and a GARP to establish a potential distribution model for the target species based on 182 known occurrence sites and 17 environmental variables. The results indicate that Maxent performed better than GARP. The mean diurnal temperature range, annual precipitation, mean air temperature of driest quarter and sunshine duration in growing season were important environmental factors influencing the distribution of C. camphora and contributed 40.9%, 23.0%, 10.5%, and 7.2% to the variation in the model contribution, respectively. Based on the models, the subtropical and temperate regions of Eastern China, where the species has been recorded, had a high suitability for this species. Under each climate change scenario, the potential geographical distribution shifted farther north and toward a higher elevation. The predicted spatial and temporal distribution patterns of this species can provide guidance for the development strategies for forest management and species protection.
Linear and Nonlinear Stability Analyses of Double-Diffusive Convection in a Vertical Brinkman Porous Enclosure under Soret and Dufour Effects
Authors: Bouachir Amel , Mamou Mahmoud , Rebhi Redha , Benissaad Smail
Published: August 2021

Analytical and numerical investigations were performed to study the influence of the Soret and Dufour effects on double-diffusive convection in a vertical porous layer filled with a binary mixture and subject to horizontal thermal and solute gradients. In particular, the study was focused on the effect of Soret and Dufour diffusion on bifurcation types from the rest state toward steady convective state, and then toward oscillatory convective state. The Brinkman-extended Darcy model and the Boussinesq approximation were employed to model the convective flow within the porous layer. Following past laboratory experiments, the investigations dealt with the particular situation where the solutal and thermal buoyancy forces were equal but acting in opposite direction to favor the possible occurrence of the rest state condition. For this situation, the onset of convection could be either supercritical or subcritical and occurred at given thresholds and following various bifurcation routes. The analytical investigation was based on the parallel flow approximation, which was valid only for a tall porous layer. A numerical linear stability analysis of the diffusive and convective states was performed on the basis of the finite element method. The thresholds of supercritical, RTCsup, and overstable, RTCover, convection were computed. In addition, the stability of the established convective flow, predicted by the parallel flow approximation, was studied numerically to predict the onset of Hopf’s bifurcation, RTCHopf, which marked the transition point from steady toward unsteady convective flows; a route towards the chaos. To support the analytical analyses of the convective flows and the numerical stability methodology and results, nonlinear numerical solutions of the full governing equations were obtained using a second-order finite difference method. Overall, the Soret and Dufour effects were seen to affect significantly the thresholds of stationary, overstable and oscillatory convection. The Hopf bifurcation was marked by secondary convective flows consisting of superposed vertical layers of opposite traveling waves. A good agreement was found between the predictions of the parallel flow approximation, the numerical solution and the linear stability results.
Evolution of Extra Virgin Olive Oil Quality under Different Storage Conditions
Authors: Mousavi Soraya , Mariotti Roberto , Stanzione Vitale , Pandolfi Saverio , Mastio Valerio , Baldoni Luciana , Cultrera Nicolò G. M.
Published: August 2021

The extent and conditions of storage may affect the stability and quality of extra virgin olive oil (EVOO). This study aimed at evaluating the effects of different storage conditions (ambient, 4 °C and −18 °C temperatures, and argon headspace) on three EVOOs (low, medium, and high phenols) over 18 and 36 months, analyzing the main metabolites at six time points. The results showed that low temperatures are able to maintain all three EVOOs within the legal limits established by the current EU regulations for most compounds up to 36 months. Oleocanthal, squalene, and total phenols were affected by storage temperatures more than other compounds and degradation of squalene and α-tocopherol was inhibited only by low temperatures. The best temperature for 3-year conservation was 4 °C, but −18 °C represented the optimum temperature to preserve the organoleptic properties. The present study provided new insights that should guide EVOO manufacturers and traders to apply the most efficient storage methods to maintain the characteristics of the freshly extracted oils for a long conservation time.
Effect of Tempering Conditions on White Sorghum Milling, Flour, and Bread Properties
Authors: Yoganandan Mohana , Bean Scott R. , Miller-Regan Rebecca , Dogan Hulya , Pulivarthi Manoj Kumar , Siliveru Kaliramesh
Published: August 2021

The effects of room temperature water, hot water, and steam tempering methods were investigated on sorghum kernel physical properties, milling, flour, and bread-making properties. Overall tempering condition and tempering moisture content were found to have a significant effect on the physical properties. Milling properties were evaluated using a laboratory-scale roller milling flowsheet consisting of four break rolls and eight reduction rolls. Room temperature tempering (18% moisture for 24 h) led to better separation of bran and endosperm without negatively impacting flour quality characteristics i.e., particle size distribution, flour yield, protein, ash, damaged starch, and moisture content. Bread produced from the flour obtained from milling sorghum kernels tempered with room temperature water (18% m.c for 24 h) and hot water (16% m.c at 60 °C for 18 h) displayed better bread-making properties i.e., high firmness, resilience, volume index, higher number of cells, and thinner cell walls when compared to other tempering conditions. Room temperature water tempering treatment (18% m.c for 24 h) could be a better pretreatment process for milling white sorghum kernels without negatively impacting the flour and bread-making quality characteristics.
Chemical and Bioactive Characterization of Spanish and Belgian Apple Pomace for Its Potential Use as a Novel Dermocosmetic Formulation
Authors: Arraibi Ana Alvarez , Liberal Ângela , Dias Maria Inês , Alves Maria José , Ferreira Isabel C. F. R. , Barros Lillian , Barreira João C. M.
Published: August 2021

Currently, there is a general trend towards reutilizing industrial by-products that would otherwise be discarded or considered as waste, aiming to explore them as alternative sources of valuable compounds. The apple pomace remaining from cider and apple juice industries represents a high-potential source of bioactive compounds with putative application in food or pharmaceutical-related products. Accordingly, the work reported herein was conducted to characterize the phenolic compounds in apple pomace from Belgium and Spain, as well as to evaluate its chemical composition and particular types of bioactivity. As a proof of concept, a new hydrogel was prepared, incorporated with the bioactive compounds and pectin extracted from apple pomace, aiming to obtain the most organic formulation possible. Independently of the extracting agent, it became evident that using lyophilization as the drying step is a better choice than thermal processes as it yielded a richer phenolic profile (fifteen individual compounds), with 5-O-caffeoylquinic acid as the major compound (66 to 114 mg/100 g dw) in Belgian samples. In general, the hydroethanolic extracts showed the strongest antioxidant and antimicrobial (particularly against Propionibacterium acnes: MIC = 2.5 mg/mL) activities. This result, together with the lipid nature of human skin, led it to be chosen as the extract type to be incorporated in the hydrogel. In general, apple pomace stood out as a valuable source of bioactive compounds, especially polyphenols and pectin, with good potential to be incorporated in dermal formulations.
Geographical Differentiation of Hom Mali Rice Cultivated in Different Regions of Thailand Using FTIR-ATR and NIR Spectroscopy
Authors: Srinuttrakul Wannee , Mihailova Alina , Islam Marivil D. , Liebisch Beatrix , Maxwell Florence , Kelly Simon D. , Cannavan Andrew
Published: August 2021

Although Hom Mali rice is considered the highest quality rice in Thailand, it is susceptible to adulteration and substitution. There is a need for rapid, low-cost and efficient analytical techniques for monitoring the authenticity and geographical origin of Thai Hom Mali rice. In this study, two infrared spectroscopy techniques, Fourier-transform infrared spectroscopy with attenuated total reflection (FTIR-ATR) and near-infrared (NIR) spectroscopy, were applied and compared for the differentiation of Thai Hom Mali rice from two geographical regions over two production years. The Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) model, built using spectral data from the benchtop FTIR-ATR, achieved 96.97% and 100% correct classification of the test dataset for each of the production years, respectively. The OPLS-DA model, built using spectral data from the portable handheld NIR, achieved 84.85% and 86.96% correct classification of the test dataset for each of the production years, respectively. Direct NIR analysis of the polished rice grains (i.e., no sample preparation) was determined as reliable for analysis of ground rice samples. FTIR-ATR and NIR spectroscopic analysis both have significant potential as screening tools for the rapid detection of fraud issues related to the geographical origin of Thai Hom Mali rice.
Tropical Fruits and Their Co-Products as Bioactive Compounds and Their Health Effects: A Review
Authors: Sayago-Ayerdi Sonia , García-Martínez Diana Laura , Ramírez-Castillo Ailin Cecilia , Ramírez-Concepción Heidi Rubí , Viuda-Martos Manuel
Published: August 2021

Tropical and subtropical fruits are recognized as a source of a high content of bioactive compounds and health promoting properties due to their nutritional composition. These beneficial health effects are related to the content of several of these bioactive compounds, mainly flavonoids and non-flavonoid phenolics. Many of these compounds are common in different tropical fruits, such as epicatechin in mango, pineapple, and banana, or catechin in pineapple, cocoa or avocado. Many studies of tropical fruits had been carried out, but in this work an examination is made in the current literature of the flavonoids and non-flavonoid phenolics content of some tropical fruits and their coproducts, comparing the content in the same units, as well as examining the role that these compounds play in health benefits.
Effect of Filter Types on Physicochemical Properties, Volatile Compounds, and Sensory Evaluations of Purified Water by Point-of-Use Water Treatment
Authors: Kim Mi-Ran , Heo JeongAe , Kim Sang Sook , Shin Eui-Cheol , Boo Chang Guk , Kwak Han Sub
Published: August 2021

This study investigated purified water from four different filter types for removing minerals, anions, and volatile organic compounds (VOCs), and affecting sensory perception and consumer acceptability. Ultrafiltration (UF), CSM-ultrafiltration (CU), alumina nanofiber (AN), and reverse osmosis (RO) filters were used for a point-of-use water treatment system with a pre-carbon filter (PR) and post-carbon filter (PO). Filters efficiently removed VOCs, which could negatively affect the sensory perception of water. The total VOC concentration of tap water (TW) (14.97 µg/Kg) was reduced by 70% by the PR, 75.3–88.7% by the PR-main filter, and >97% by the PR-RO-PR. Using the polarized sensory position test, the subjects clearly discriminated TW from the samples; however, most of the purified water was not. The difference in the mean ratings of consumer acceptability among the purified samples was <1 except for PR-RO-PO in consumer testing. These results suggested that although there are differences in the capability of different filter types to eliminate minerals, anions, and VOCs, overall consumers did not identify sensory differences among them, and demonstrated similar consumer acceptability of the purified water produced. Simply applying a pre-carbon filter for TW treatment is enough to minimize VOCs, which negatively influence consumer acceptability.
Incorporation of Low Molecular Weight Chitosan in a Low-Fat Beef Burger: Assessment of Technological Quality and Oxidative Stability
Authors: Amoli Pourya , Hadidi Milad , Hasiri Zahra , Rouhafza Arman , Jelyani Aniseh , Hadian Zahra , Khaneghah Amin , Lorenzo José
Published: August 2021

In the present work, incorporating low molecular weight chitosan (LMWCH) (0, 0.5, 1, and 2%) as a fat replacer into low-fat beef burgers and technological, textural, and oxidative stability were investigated. The weight loss and shrinkage of samples decreased with the increase of LMWCH concentration. In contrast, the water-holding capacity and color of burgers were enhanced by the addition of LMWCH. The instrumental TPA results indicated an increase in the LMWCH levels, significantly increasing the hardness, springiness, and gumminess but decreasing the cohesiveness of low-fat beef burgers. The TBARS and peroxide values and free fatty acid content in the burgers supplemented with LMWCH increase slower than the control sample during refrigerated storage.
Effects of Vacuum Frying on the Preparation of Ready-to-Heat Batter-Fried and Sauced Chub Mackerel (Scomber japonicus)
Authors: Tirtawijaya Gabriel , Lee Mi-Jeong , Negara Bertoka Fajar Surya Perwira , Cho Woo-Hee , Sohn Jae-Hak , Kim Jin-Soo , Choi Jae-Suk
Published: August 2021

Chub mackerel (CM) is a commercial fish in Korea, owing to its availability and nutritional values. This study aimed to develop a ready-to-heat (RTH) Korean preparation of CM, known as Godeungo gangjeong. We utilized vacuum frying technology to fry the CM and evaluated its quality. Conventional frying with a deep fryer was performed in parallel to assess the superiority of the vacuum fryer. We optimized the frying conditions of vacuum frying (VBF) and deep frying (DBF) using response surface methodology. At optimum conditions of 95 °C for 7 min 42 s, VBF produced better sensory, chemical, and microbial properties than DBF at 190 °C for 5 min 30 s. The nutritional values, including amino acid and fatty acid contents, were investigated and found to be higher in VBF than in DBF. Sensory properties also showed better scores on VBF than DBF, especially in appearance, aroma, taste, and overall acceptability. The VBF produced lower volatile basic nitrogen (VBN), thiobarbituric acid reactive substances (TBARS), and total bacterial count (TBC) than DBF. The findings confirmed that vacuum frying is a better option to produce RTH Godeungo gangjeong, since it provides less oxidation and maintains the product quality. Using the Arrhenius approach, the product was concluded to preserve both quality and safety for 9 months of storage at −18 °C.
Casein Micelles as an Emerging Delivery System for Bioactive Food Components
Authors: Sadiq Uzma , Gill Harsharn , Chandrapala Jayani
Published: August 2021

Bioactive food components have potential health benefits but are highly susceptible for degradation under adverse conditions such as light, pH, temperature and oxygen. Furthermore, they are known to have poor solubilities, low stabilities and low bioavailabilities in the gastrointestinal tract. Hence, technologies that can retain, protect and enable their targeted delivery are significant to the food industry. Amongst these, microencapsulation of bioactives has emerged as a promising technology. The present review evaluates the potential use of casein micelles (CMs) as a bioactive delivery system. The review discusses in depth how physicochemical and techno-functional properties of CMs can be modified by secondary processing parameters in making them a choice for the delivery of food bioactives in functional foods. CMs are an assembly of four types of caseins, (αs1, αs2, β and κ casein) with calcium phosphate. They possess hydrophobic and hydrophilic properties that make them ideal for encapsulation of food bioactives. In addition, CMs have a self-assembling nature to incorporate bioactives, remarkable surface activity to stabilise emulsions and the ability to bind hydrophobic components when heated. Moreover, CMs can act as natural hydrogels to encapsulate minerals, bind with polymers to form nano capsules and possess pH swelling behaviour for targeted and controlled release of bioactives in the GI tract. Although numerous novel advancements of employing CMs as an effective delivery have been reported in recent years, more comprehensive studies are required to increase the understanding of how variation in structural properties of CMs be utilised to deliver bioactives with different physical, chemical and structural properties.
Control and Robust Stabilization at Unstable Equilibrium by Fractional Controller for Magnetic Levitation Systems
Authors: Ataşlar-Ayyıldız Banu , Karahan Oğuzhan , Yılmaz Serhat
Published: August 2021

The problem of control and stabilizing inherently non-linear and unstable magnetic levitation (Maglev) systems with uncertain equilibrium states has been studied. Accordingly, some significant works related to different control approaches have been highlighted to provide robust control and enhance the performance of the Maglev system. This work examines a method to control and stabilize the levitation system in the presence of disturbance and parameter variations to minimize the magnet gap deviation from the equilibrium position. To fulfill the stabilization and disturbance rejection for this non-linear dynamic system, the fractional order PID, fractional order sliding mode, and fractional order Fuzzy control approaches are conducted. In order to design the suitable control outlines based on fractional order controllers, a tuning hybrid method of GWO–PSO algorithms is applied by using the different performance criteria as Integrated Absolute Error (IAE), Integrated Time Weighted Absolute Error (ITAE), Integrated Squared Error (ISE), and Integrated Time Weighted Squared Error (ITSE). In general, these objectives are used by targeting the best tuning of specified control parameters. Finally, the simulation results are presented to determine which fractional controllers demonstrate better control performance, achieve fast and robust stability of the closed-loop system, and provide excellent disturbance suppression effect under nonlinear and uncertainty existing in the processing system.
Benefits of Exome Sequencing in Children with Suspected Isolated Hearing Loss
Authors: Van Heurck Roxane , Carminho-Rodrigues Maria Teresa , Ranza Emmanuelle , Stafuzza Caterina , Quteineh Lina , Gehrig Corinne , Hammar Eva , Guipponi Michel , Abramowicz Marc , Senn Pascal , Guinand Nils , Cao-Van Helene , Paoloni-Giacobino Ariane
Published: August 2021

Purpose: Hearing loss is characterized by an extensive genetic heterogeneity and remains a common disorder in children. Molecular diagnosis is of particular benefit in children, and permits the early identification of clinically-unrecognized hearing loss syndromes, which permits effective clinical management and follow-up, including genetic counselling. Methods: We performed whole-exome sequencing with the analysis of a panel of 189 genes associated with hearing loss in a prospective cohort of 61 children and 9 adults presenting mainly with isolated hearing loss. Results: The overall diagnostic rate using exome sequencing was 47.2% (52.5% in children; 22% in adults). In children with confirmed molecular results, 17/32 (53.2%) showed autosomal recessive inheritance patterns, 14/32 (43.75%) showed an autosomal dominant condition, and one case had X-linked hearing loss. In adults, the two patients showed an autosomal dominant inheritance pattern. Among the 32 children, 17 (53.1%) had nonsyndromic hearing loss and 15 (46.7%) had syndromic hearing loss. One adult was diagnosed with syndromic hearing loss and one with nonsyndromic hearing loss. The most common causative genes were STRC (5 cases), GJB2 (3 cases), COL11A1 (3 cases), and ACTG1 (3 cases). Conclusions: Exome sequencing has a high diagnostic yield in children with hearing loss and can reveal a syndromic hearing loss form before other organs/systems become involved, allowing the surveillance of unrecognized present and/or future complications associated with these syndromes.
Prediction of Parkinson’s Disease Risk Based on Genetic Profile and Established Risk Factors
Authors: Chairta Paraskevi P. , Hadjisavvas Andreas , Georgiou Andrea N. , Loizidou Maria A. , Yiangou Kristia , Demetriou Christiana A. , Christou Yiolanda P. , Pantziaris Marios , Michailidou Kyriaki , Zamba-Papanicolaou Eleni
Published: August 2021

Background: Parkinson’s disease (PD) is a neurodegenerative disorder, and literature suggests that genetics and lifestyle/environmental factors may play a key role in the triggering of the disease. This study aimed to evaluate the predictive performance of a 12-Single Nucleotide Polymorphisms (SNPs) polygenic risk score (PRS) in combination with already established PD-environmental/lifestyle factors. Methods: Genotypic and lifestyle/environmental data on 235 PD-patients and 464 controls were obtained from a previous study carried out in the Cypriot population. A PRS was calculated for each individual. Univariate logistic-regression analysis was used to assess the association of PRS and each risk factor with PD-status. Stepwise-regression analysis was used to select the best predictive model for PD combining genetic and lifestyle/environmental factors. Results: The 12-SNPs PRS was significantly increased in PD-cases compared to controls. Furthermore, univariate analyses showed that age, head injury, family history, depression, and Body Mass Index (BMI) were significantly associated with PD-status. Stepwise-regression suggested that a model which includes PRS and seven other independent lifestyle/environmental factors is the most predictive of PD in our population. Conclusions: These results suggest an association between both genetic and environmental factors and PD, and highlight the potential for the use of PRS in combination with the classical risk factors for risk prediction of PD.
A Novel Missense Mutation in TNNI3K Causes Recessively Inherited Cardiac Conduction Disease in a Consanguineous Pakistani Family
Authors: Ramzan Shafaq , Tennstedt Stephanie , Tariq Muhammad , Khan Sheraz , Noor Ul Ayan Hafiza , Ali Aamir , Munz Matthias , Thiele Holger , Korejo Asad Aslam , Mughal Abdul Razzaq , Jamal Syed Zahid , Nürnberg Peter , Baig Shahid Mahmood , Erdmann Jeanette , Ahmad Ilyas
Published: August 2021

Cardiac conduction disease (CCD), which causes altered electrical impulse propagation in the heart, is a life-threatening condition with high morbidity and mortality. It exhibits genetic and clinical heterogeneity with diverse pathomechanisms, but in most cases, it disrupts the synchronous activity of impulse-generating nodes and impulse-conduction underlying the normal heartbeat. In this study, we investigated a consanguineous Pakistani family comprised of four patients with CCD. We applied whole exome sequencing (WES) and co-segregation analysis, which identified a novel homozygous missense mutation (c.1531T>C;(p.Ser511Pro)) in the highly conserved kinase domain of the cardiac troponin I-interacting kinase (TNNI3K) encoding gene. The behaviors of mutant and native TNNI3K were compared by performing all-atom long-term molecular dynamics simulations, which revealed changes at the protein surface and in the hydrogen bond network. Furthermore, intra and intermolecular interaction analyses revealed that p.Ser511Pro causes structural variation in the ATP-binding pocket and the homodimer interface. These findings suggest p.Ser511Pro to be a pathogenic variant. Our study provides insights into how the variant perturbs the TNNI3K structure-function relationship, leading to a disease state. This is the first report of a recessive mutation in TNNI3K and the first mutation in this gene identified in the Pakistani population.
Qualitative Study of Community Pharmacists’ and General Practitioners’ Views toward Pharmacovigilance in Lithuania
Authors: Valinciute-Jankauskiene Agne , Kubiliene Loreta
Published: August 2021

Lithuania is the leader in pharmacovigilance among the three Baltic countries. However, comparisons with other European countries are difficult because the reported number of adverse drug reactions (ADRs) in Lithuania is too low to rely on in terms of the annual use of medicines by the population over the year. The aim of this study was to explore challenges related to the understanding and practices of general practitioners and community pharmacists in reporting ADRs in Lithuania. The qualitative study approach of face-to-face interviews was used. All interviews were recorded, transcribed verbatim, and thematically analyzed. Twelve interviews with general practitioners and community pharmacists were conducted from March 2020 through December 2020. All participants had a basic knowledge of pharmacovigilance, but only four participants reported ADRs to the interviewer. Six main barriers regarding ADR reporting were identified, and appropriate interventions were suggested. The importance of collaboration between physicians and pharmacists was highlighted, and the need for guidelines supporting collaboration was expressed. Medications are becoming more complex, and comprehensive medication management is key for the optimization of patient outcomes. Our results reveal the need to improve and innovate the current pharmacovigilance system at all levels, starting from education for pharmacy and healthcare students and continuing through the development of ADR procedures.
Different Roles of Telehealth and Telemedicine on Medical Tourism: An Empirical Study from Azerbaijan
Authors: Gu Dongxiao , Humbatova Gunay , Xie Yi , Yang Xuejie , Zolotarev Oleg , Zhang Gongrang
Published: August 2021

With the rapid progress in mobile healthcare and Internet medicine, the impact of telehealth and telemedicine on the satisfaction of patients and their willingness to travel has become a focus of the academic research community. This study analyses the differences between telehealth and telemedicine and their role in medical tourism. We examine how the information quality and communication quality of telehealth and telemedicine influence patient satisfaction, and their effects on patients’ willingness to undertake medical travel and on their medical travel behaviours. We conducted an empirical study on the use of telehealth and telemedicine and on medical travel behaviour in Azerbaijan using a survey for data collection. A total of 500 results were collected and analysed using SmartPLS 3.0. Results show that (1) the communication quality and information quality of telehealth and telemedicine and their effects on satisfaction have significantly positive influences on willingness to undertake medical travel; (2) the psychological expectations of value and cost (perceived value and perceived cost) have a positive influence on medical travel; and (3) willingness to participate in medical travel positively influences medical travel behaviour. Moreover, results of this study have implications for research on, and the practice of, using telehealth and telemedicine as they relate to medical tourism. This research may help improve knowledge about telehealth and telemedicine and understand the differences between them in detail. This empirical research model may also be useful for researchers from other countries who wish to measure medical travel behaviour.
Placement Recommendations for Single Kinect-Based Motion Capture System in Unilateral Dynamic Motion Analysis
Authors: Cai Laisi , Liu Dongwei , Ma Ye
Published: August 2021

Low-cost, portable, and easy-to-use Kinect-based systems achieved great popularity in out-of-the-lab motion analysis. The placement of a Kinect sensor significantly influences the accuracy in measuring kinematic parameters for dynamics tasks. We conducted an experiment to investigate the impact of sensor placement on the accuracy of upper limb kinematics during a typical upper limb functional task, the drinking task. Using a 3D motion capture system as the golden standard, we tested twenty-one Kinect positions with three different distances and seven orientations. Upper limb joint angles, including shoulder flexion/extension, shoulder adduction/abduction, shoulder internal/external rotation, and elbow flexion/extension angles, are calculated via our developed Kinect kinematic model and the UWA kinematic model for both the Kinect-based system and the 3D motion capture system. We extracted the angles at the point of the target achieved (PTA). The mean-absolute-error (MEA) with the standard represents the Kinect-based system’s performance. We conducted a two-way repeated measure ANOVA to explore the impacts of distance and orientation on the MEAs for all upper limb angles. There is a significant main effect for orientation. The main effects for distance and the interaction effects do not reach statistical significance. The post hoc test using LSD test for orientation shows that the effect of orientation is joint-dependent and plane-dependent. For a complex task (e.g., drinking), which involves body occlusions, placing a Kinect sensor right in front of a subject is not a good choice. We suggest that place a Kinect sensor at the contralateral side of a subject with the orientation around 30∘ to 45∘ for upper limb functional tasks. For all kinds of dynamic tasks, we put forward the following recommendations for the placement of a Kinect sensor. First, set an optimal sensor position for capture, making sure that all investigated joints are visible during the whole task. Second, sensor placement should avoid body occlusion at the maximum extension. Third, if an optimal location cannot be achieved in an out-of-the-lab environment, researchers could put the Kinect sensor at an optimal orientation by trading off the factor of distance. Last, for those need to assess functions of both limbs, the users can relocate the sensor and re-evaluate the functions of the other side once they finish evaluating functions of one side of a subject.
Korean Nursing Students’ Emotional Response Types to Pandemic: Application of Q-Methodology
Authors: Seong Mihyeon
Published: August 2021

The aim of this study is to investigate the emotional responses of nursing students to the COVID-19 pandemic and the characteristics of these responses using the Q-methodology. The focus of the Q-methodology is to discover research participants’ subjective viewpoints. In May 2021, 50 Korean nursing students from first year to fourth year were selected to participate in the study, and data were collected by asking them to classify 37 selected Q-statements in a normal distribution on a 9-point scale. In the final analysis, a P-sample of 38 nursing students was used. The emotional responses of the Korean nursing students were categorized into four types: self-protection (Type 1), pessimism about the current situation (Type 2), realistic optimism (Type 3), and developmental-seeking (Type 4). The four factors accounted for 39% of the total variance. The individual explanatory powers of the four factors were 8%, 12%, 13%, and 6%, respectively. Thus, the study explored the subjectivity of emotional responses of Korean nursing students to the pandemic. The study recommends considering the results for intervention programs that are designed to prepare nursing students for future health crises and pandemics.
Reviving Alexander Calder’s Man-Eater with Pennants: A Technical Examination of the Original Paint Palette
Authors: Haddad Abed , Randall Megan , Zycherman Lynda , Martins Ana
Published: August 2021

Man-Eater with Pennants, a rarely exhibited sculpture in Alexander Calder’s oeuvre, was commissioned by The Museum of Modern Art (MoMA) and installed in 1945. To exhibit the large standing mobile in Alexander Calder: Modern from the Start (2021), the derelict sculpture had to be remediated; this initiated a collaborative investigation with conservation scientists, conservators, curators, and the Calder Foundation into the original paint colors hidden beneath layers of repaint. XRF analysis was carried out to elucidate the paints’ composition, followed by sampling for analysis to assess the paint stratigraphy and binders. Scrapings were analyzed by µ-FTIR and Raman spectroscopies; cross sections were examined with optical microscopy and analyzed with SEM-EDS. Analysis differentiated between the original paints, which contain Prussian blue, parachlor red, chrome yellow, and the many layers of overpaint, which contain titanium white, molybdate orange, a variety of β-Naphthol reds, red lead, and ultramarine. A model for Man-Eater, Mobile with 14 Flags, is also part of the museum’s collection, and was first considered as a point of reference for the original colors. Similar analysis, however, indicates that the maquette was painted after the Man-Eater was first installed, therefore is not representative of the original colors. In addition to investigating an early primary palette for Calder’s outdoor sculptures, this study helped develop the plan for the restoration of the original color scheme of Man-Eater.
The Application of Nitrogen Fertilization and Foliar Spraying with Calcium and Boron Affects Growth Aspects, Chemical Composition, Productivity and Fruit Quality of Strawberry Plants
Authors: Mohamed Mustafa. H. M. , Petropoulos Spyridon A. , Ali Maha Mohamed Elsayed
Published: August 2021

Strawberries are rich in antioxidants and plant components to enhance cardiac health and regulate blood sugar. This experiment investigates the effects of N fertilization and foliar spraying with calcium (Ca) and boron (B) on growth, chemical composition of plant foliage, fruit yield, and quality of strawberry plants (Fragaria × ananassa Duch. cv. Fortuna). This experiment includes 12 treatments from the combination between four N fertilizers treatments (combinations of mineral and organic fertilizers) and three foliar spraying treatments with Ca+B at 2 and 4 mL/L in addition to the control treatment (spraying with tap water). Results show that the treatment where 50% of the recommended dose of N (50% RDN) was applied with mineral fertilizer + 100% organic N was the most effective one since it induced the highest values of plant height, number of crowns per plant, number of leaves per plant, fresh and dry weight per plant, especially when combined with the foliar application of Ca+B at the highest tested rate (4 mL/L). The highest values of N, P, K, Ca, B, and total carbohydrate contents were also scored for the combination of 50% RDN + 100% organic N and the foliar spraying with Ca+B at 4 mL/L in both growing seasons. In addition, the highest values of fruit yield per plant and per hectare, exportable fruit yield/ha, fruit firmness, fruit TSS, Vitamin C, total sugars, and anthocyanin, as well as the lowest values of total acidity were recorded for the combined treatment of 50% RDN + 100% organic and Ca+B at the highest rate (4 mL/L). The highest recorded value of length, diameter, and weight of fruit were scored by fertilizing strawberry plants with the recommended dose of mineral N (100% RDN) and spraying with Ca+B at the highest rate in both growing seasons. In conclusion, it could be suggested that fertilization of strawberry plants with half the recommended dose with mineral N fertilizer + 100% organic fertilizer and foliar spraying with Ca+B at 4 mL/L increases plant growth and improve yield parameters and fruit quality of the strawberry plants.
Fruit Colour and Novel Mechanisms of Genetic Regulation of Pigment Production in Tomato Fruits
Authors: Gonzali Silvia , Perata Pierdomenico
Published: August 2021

Fruit colour represents a genetic trait with ecological and nutritional value. Plants mainly use colour to attract animals and favour seed dispersion. Thus, in many species, fruit colour coevolved with frugivories and their preferences. Environmental factors, however, represented other adaptive forces and further diversification was driven by domestication. All these factors cooperated in the evolution of tomato fruit, one of the most important in human nutrition. Tomato phylogenetic history showed two main steps in colour evolution: the change from green-chlorophyll to red-carotenoid pericarp, and the loss of the anthocyanic pigmentation. These events likely occurred with the onset of domestication. Then spontaneous mutations repeatedly occurred in carotenoid and phenylpropanoid pathways, leading to colour variants which often were propagated. Introgression breeding further enriched the panel of pigmentation patterns. In recent decades, the genetic determinants underneath tomato colours were identified. Novel evidence indicates that key regulatory and biosynthetic genes undergo mechanisms of gene expression regulation that are much more complex than what was imagined before: post-transcriptional mechanisms, with RNA splicing among the most common, indeed play crucial roles to fine-tune the expression of this trait in fruits and offer new substrate for the rise of genetic variables, thus providing further evolutionary flexibility to the character.
Socioeconomic Inequalities in Chronic Liver Diseases and Cirrhosis Mortality in European Urban Areas before and after the Onset of the 2008 Economic Recession
Authors: Borrell Carme , Palència Laia , Bosakova Lucia , Gotsens Mercè , Morrison Joana , Costa Claudia , Dzurova Dagmar , Deboosere Patrick , Lustigova Michala , Marí-Dell’Olmo Marc , Rodopoulou Sophia , Santana Paula
Published: August 2021

Objective: To analyse the trends in chronic liver diseases and cirrhosis mortality, and the associated socioeconomic inequalities, in nine European cities and urban areas before and after the onset of the 2008 financial crisis. Methods: This is an ecological study of trends in three periods of time: two before (2000–2003 and 2004–2008), and one after (2009–2014) the onset of the economic crisis. The units of analysis were the geographical areas of nine cities or urban areas in Europe. We analysed chronic liver diseases and cirrhosis standardised mortality ratios, smoothing them with a hierarchical Bayesian model by each city, area, and sex. An ecological regression model was fitted to analyse the trends in socioeconomic inequalities, and included the socioeconomic deprivation index, the period, and their interaction. Results: In general, chronic liver diseases and cirrhosis mortality rates were higher in men than in women. These rates decreased in all cities during the financial crisis, except among men in Athens (rates increased from 8.50 per 100,000 inhabitants during the second period to 9.42 during the third). Socioeconomic inequalities in chronic liver diseases and cirrhosis mortality were found in six cities/metropolitan areas among men, and in four among women. Finally, in the periods studied, such inequalities did not significantly change. However, among men they increased in Turin and Barcelona and among women, several cities had lower inequalities in the third period. Conclusions: There are geographical socioeconomic inequalities in chronic liver diseases and cirrhosis mortality, mainly among men, that did not change during the 2008 financial crisis. These results should be monitored in the long term.