bluuebunny/crossref_metadata_2025 huggingface dataset:
The BE(2)-M17 cell line has a better dopaminergic phenotype than the traditionally used for Parkinson´s research SH-SY5Y, which is mostly serotonergic
Authors: Carvajal-Oliveros Angel , Uriostegui-Arcos Maritere , Zurita Mario , Melchy-Perez Erika I. , Narváez-Padilla Verónica , Reynaud Enrique
Published: March 2022

SH-SY5Y is a cell line derived from human neuroblastoma. It is one of the most widely used in vitro models to study Parkinson’s disease. Surprisingly, it has been found that it does not develop a dopaminergic phenotype after differentiation, questioning its usefulness as a Parkinson’s model. There are other in vitro models with better dopaminergic characteristics. BE (2)-M17 is a human neuroblastoma cell line that differentiates when treated with retinoic acid. We compared the dopaminergic and serotonergic properties of both cell lines. BE (2)-M17 has higher basal levels of dopaminergic markers and acquires a serotonergic phenotype during differentiation while maintaining the dopaminergic phenotype. SH-SY5Y has higher basal levels of serotonergic markers but does not acquire a dopaminergic phenotype upon differentiation.
Application of different approaches to generate virtual patient populations for the Quantitative Systems Pharmacology model of erythropoiesis
Authors: Kolesova Galina , Stepanov Alexander , Lebedeva Galina , Demin Oleg
Published: March 2022

In a standard situation, a quantitative systems pharmacology model describes a “reference patient,” and the model parameters are fixed values allowing only the mean values to be described. However, the results of clinical trials include a description of variability in patients’ responses to a drug, which is typically expressed in terms of conventional statistical parameters, such as standard deviations (SDs) from mean values. Therefore, in this study, we propose and compare 4 different approaches: (1) Monte Carlo Markov Chain (MCMC); (2) model fitting to Monte Carlo sample; (3) population of clones; (4) stochastically bounded selection to generate virtual patient populations based on experimentally measured mean data and SDs. We applied these approaches to generate virtual patient populations in the QSP model of erythropoiesis. According to the results of our research, stochastically bounded selection showed slightly better results than the other 3 methods as it allowed the description of any number of patients from clinical trials and could be applied in the case of complex models with a large number of variable parameters.
Robotic natural orifice specimen extraction surgery for colorectal cancer: an open-label, single-arm study
Authors: Zhong Yun , Zhang Hao-bo , Luo Rui , Li Tai-yuan , Liu Dong-ning
Published: March 2022

Background: Robotic natural orifice specimen extraction surgery (NOSES) is a minimally invasive surgery for colorectal cancer patients. As an emerging procedure, its safety and feasibility require thorough investigation. This study aimed to evaluate preliminary data and provide evidence for the therapeutic benefits and clinical applicability of robotic NOSES in colorectal cancer patients.Methods: This was an open-label single-arm study that recruited patients with colorectal cancer and treated them with robotic NOSES, which was performed by three methods of specimen extraction: a: transanal specimen eversion and extra-abdominal resection technique (EVER); b: translumenal specimen extraction and extra-abdominal resection technique (EXER); c: intra-abdominal specimen resection and translumenal extraction technique (IREX). The primary outcome was the complication rate. Between September 1, 2019, and May 31, 2020, 118 individuals were screened, and 101 were included in the intention-to-treat population. Lastly, after further evaluation, 91 individuals were included in the per-protocol population. Safety analyses were performed using SPSS 22.0 (SPSS, Chicago, USA). P-value < 0.05 was considered statistically significant.Results: A total of 118 patients were screened, and 101 were enrolled for the robotic NOSES. Ten patients switched to traditional robotic-assisted surgery. Hypertension [13 (14.29%)] was the most common comorbidity. The overall complication rate was 18.68%, and the most common complication was anastomotic fistula (B and C level) [8 (8.79%)]. There was no significant difference in complication rate among the three methods of specimen extraction in the subgroup analysis (P = 0.638).Conclusion: Robotic NOSES can provide feasibility for colorectal cancer patients to a certain extent. The most prevalent surgical complication of robotic NOSES for colorectal cancer patients was anastomotic fistula (B and C level) [8 (8.79%)].
1,3-Diphenylurea derivatives inhibit cellular entry of influenza A virus and SARS-CoV-2
Authors: Kumar Nirmal , Taily Irshad , Singh Charandeep , Kumar Sahil , Sharma Anshul , Singh Priyanka , Thakur Krishan , Ringe Rajesh , Banerjee Prabal , Banerjee Indranil
Published: March 2022

Influenza A virus (IAV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are highly contagious pathogens that cause severe respiratory illnesses, often leading to hospitalization and death. Rapid emergence of mutant viral strains and waning immunity in the vaccinated individuals represent major challenges in the global fight against both the viruses. Complementing vaccine protection, a search for new therapeutic agents, effective against the existing and emerging viral strains, is an urgent priority. In this study, we identified 1,3-diphenylurea derivatives (DPUDs) as a new class of potential antiviral agents that robustly inhibited infections by different strains of IAV and SARS-CoV-2. Our study revealed, DPUDs primarily target the host cell entry of the viruses. That DPUDs affect the host processes exploited by IAV and SARS-CoV-2 for cellular entry, they are expected to be broadly effective against other related viruses that co-opt the same entry pathways.
Identifying genes associated with abiotic stress tolerance suitable for CRISPR/Cas9 editing in upland rice cultivars adapted to acid soils (version 2)
Authors: Barrero Luz Stella , Willmann Matthew , Craft Eric , Manna-Akther Kazi , Harrington Sandra , Garzon-Martinez Gina A. , Glahn Raymond , Piñeros Miguel A. , McCouch Susan
Published: March 2022

Five genes of large phenotypic effect known to confer abiotic stress tolerance in rice were selected to characterize allelic variation in commercial Colombian tropical japonica upland rice cultivars adapted to drought-prone acid soil environments (cv. Llanura11 and Porvenir12). Allelic variants of the genes ART1, DRO1, SUB1A, PSTOL1, and SPDT were characterized by PCR and/or Sanger sequencing in the two upland cultivars and compared with the Nipponbare and other reference genomes. Two genes were identified as possible targets for gene editing: SUB1A, Submergence 1A, to improve tolerance to flooding, and SPDT (SULTR3;4), SULTR-like Phosphorus Distribution Transporter, to improve phosphorus utilization efficiency and grain quality. Based on technical and regulatory considerations, SPDT was targeted for editing. The two upland cultivars were shown to carry the SPDT wild-type (non-desirable) allele based on sequencing, RNA expression, and phenotypic evaluations under hydroponic and greenhouse conditions. A gene deletion was designed using the CRISPR/Cas9system and specialized reagents were developed for SPDT editing, including vectors targeting the gene and a protoplast transfection transient assay. The desired edits were confirmed in protoplasts and serve as the basis for ongoing plant transformation experiments aiming to improve the P-use efficiency of upland rice grown in acidic soils.
Ectopic Pancreatic Islets in Splenopancreatic Ligament: A Case Report
Authors: Aich Rajarshi , Sutradhar Arpita , Khan Enam Murshed , Maitra Sudipta Kumar , Gupta Shaikat
Published: March 2022

Heterotopic or ectopic pancreas is pancreatic tissue present at an abnormal location which is not connected to the main pancreas. They are predominantly located in the gastrointestinal tract, with stomach being the most common site. Mostly asymptomatic, they are usually detected incidentally during surgeries for other conditions. Preoperative diagnosis is very difficult and the definitive diagnosis is histopathological examination of the suspected lesion. Surgical resection is curative and it should be done in asymptomatic cases for diagnostic confirmation and prevention of complications. Here, we present a case of ectopic pancreas in a 59-year-old female in the splenopancreatic ligament which was incidentally identified during histopathological examination of specimen excised for serous cystadenoma of pancreas.
Rapid Deployment of Nasopharyngeal Test Swabs Within the US Department of Veterans Affairs
Authors: Grindle Garret G. , Strollo Patrick , Swiatkowski Robert A. , Sonel Ali , Kaplan John , Exckstein Ian , Cooper Rory A.
Published: March 2022

The COVID-19 pandemic placed tremendous strain on people, medical supply chains, and healthcare systems. The US Department of Veterans Affairs (VA) had to take extraordinary efforts to protect the veterans that it serves. The goal of this project was to rapidly deploy safe and effective
nasopharyngeal (NP) test swabs to enable expanded testing for COVID-19 with a US veteran population. This manuscript describes the technical steps to achieving this goal. VA had internal additive manufacturing resources for many of the common processes and materials. Once the process and material
were selected, the design of the NP swab geometry for effectiveness, strength, and maximum throughput commenced. A NP swab with tip geometry that resembles thin, rounded, stacked disks was selected for the design after the clinical members of the team reviewed several iterations of prototypes.
Following the ASTM D790 test, the NP swabs were examined using a 10-power digital microscope to check for micro-fractures and surface finish. Once the NP swabs passed the initial quality assurance process, the NP swabs required cleaning, sterilization, and packaging. Subsequently, each NP
swab package was labeled for identification. Approximately 30,000 NP swabs were produced with around 18,000 of them made available for immediate deployment. The effort showed that additive manufacturing along with good professional practice by engineers and healthcare professionals could rapidly
respond to a shortage of NP swabs. Internal additive manufacturing and other digital manufacturing technologies should be part of an overall strategy to increase the robustness of healthcare delivery in emergency situations.
Assessment of Basic Measures Instituted to Curtail the Spread of COVID-19 on Construction Sites in Lagos, Nigeria
Authors:   Department of Building Sciences, Tshwane University of Technology, SOUTH AFRICA, Adebowale Oluseyi Julius , Simpeh Fredrick ,   Department of Quantity Surveying and Construction Management, University of the Free State, SOUTH AFRICA, Kukoyi Patricia Omega ,   Department of Building, University of Lagos, NIGERIA, Agumba Justus Ngala ,   Department of Building Sciences, Tshwane University of Technology, SOUTH AFRICA
Published: March 2022

The COVID-19 pandemic has altered every industry’s operation and safety requirements, including that of the construction industry. Different measures are thus required to mitigate the spread of the disease among construction site workers. This article explores the basic measures construction organisations have instituted at construction project sites to curb the spread of the virus among construction site workers. A qualitative research method was adopted for the study, while data were collected through open-ended questions. Summative content analysis was employed to analyse the qualitative data and determine the frequency of keywords that were further translated to quantitative measurements. The study determined that construction organisations have largely instituted some basic measures to curb the spread of COVID-19 on sites, whereas most organisations were lacking in the area of social distancing. COVID-19 is still new; therefore, the body of knowledge is still in the infancy stage. To date, there is no evidence of empirical studies that addressed the compliance level of Nigerian construction organisations with basic measures for curtailing the spread of COVID-19. Policymakers could adopt the interventions arising from this study to mitigate the spread of the virus.
AS PRÁTICAS DE HOSPITALIDADE NA UNIVERSIDADE FEDERAL DO MARANHÃO: ANÁLISE DA PERCEPÇÃO DOS DISCENTES INTERNACIONAIS EM RELAÇÃO AO RECEBER BEM
Authors: Santos Saulo Ribeiro dos , Galvão Alessandra Martins , Leite Ângela Roberta Lucas
Published: March 2022

O bem receber tem-se traduzido e evidenciado fortemente em grandes organizações, a incluir as universidades públicas, pois a valorização das relações através de práticas de hospitalidade tem sido um dos principais meios de acolhimento para com os discentes estrangeiros. Diante do exposto, objetiva-se neste artigo compreender a percepção dos discentes estrangeiros, participantes do Programa de Alianças para a Educação e a Capacitação (PAEC) e do Programa de Estudantes-Convênio de Graduação (PEC-G), que estudam na Universidade Federal do Maranhão (UFMA), Campus Dom Delgado, São Luís, a respeito das práticas de hospitalidade promovidas pela referida instituição. A pesquisa, de abordagem qualitativa, tem como base o estudo de caso. Para coleta de dados, utilizou-se o questionário, aplicado com 20 discentes através do uso da ferramenta gratuita denominada Google Forms. Os resultados apontam que a maioria dos estudantes é participante do PEC-G, oriundos de países que fazem parte do continente Africano e América Latina, tendo como fluxo maior de ingressantes no semestre de 2018.1Além disso, os discentes apontam que existe um longo caminho na UFMA para o desenvolvimento de práticas de hospitalidade mais sólidas com discentes estrangeiros.
Personalización del proceso de adquisición de la competencia en comunicación lingüística mediante el empleo de los serious games. Diferencias en función del género
Authors: Sánchez Castro Susana , Pascual Sevillano Mª Ángeles
Published: March 2022

El sistema educativo actual tiende hacia un aprendizaje activo por parte del estudiante enfocado al desarrollo de competencias genéricas y específicas, buscando la personalización y ajuste a las diferencias individuales. En este contexto, los serious games se convierten en una alternativa óptima como estrategia de enseñanza personalizada y, por tanto, capaz de dar respuesta a poblaciones vulnerables desde una orientación de Educación flexible. La presente investigación tiene como objetivo determinar si un diseño planificado, sistematizado y personalizado basado en serious games puede contribuir al desarrollo de la competencia en comunicación lingüística en Educación Primaria. El trabajo tiene en cuenta la variable género con el fin de concluir si esta variable demanda a los programas una estrategia de personalización específica. El estudio realizado sigue un diseño cuasiexperimental con pretest-postest llevada a cabo durante 3 meses en una muestra compuesta por 78 estudiantes de 5 aulas ordinarias de 4 centros educativos de Educación Primaria. Los resultados evidencian un incremento en las submaterias de la competencia en comunicación lingüística evaluadas a través de los programas, así como una mejora en las calificaciones académicas. Se constata además que no hay diferencias reseñables en función del género en cuanto a la competencia en comunicación lingüística ni tampoco en cuanto al beneficio obtenido tras la utilización de los serious games seleccionados.
Diseño participativo a través de la herramienta CoAnnotation en las Prácticas Externas universitarias
Authors: Domene Martos Soledad , Rodríguez-Gallego Margarita R. , Salas Ruíz Sandra , Corujo-Vélez María del Carmen
Published: March 2022

Con el objetivo de mejorar la experiencia de la asignatura Prácticas Externas I, del Grado de Pedagogía de la Facultad de Ciencias de la Educación de la Universidad de Sevilla y para cubrir las necesidades sentidas por el profesorado y alumnado, se han llevado a cabo, durante el curso 2020-21, dos propuestas: blog colaborativo y simulaciones-videoconferencia para llevar a cabo un aprendizaje  flexible en el aprendizaje de esta asignatura. Tras la creación y publicación de un vídeo en CoAnnotation, los miembros de la RedTicPraxis a través de un diseño participativo realizaron valoraciones de acuerdo con el objetivo planteado. El procedimiento seguido para el análisis de datos recogidos en CoAnnotation ha consistido en un sistema categorial, creado a través de las etiquetas y el software utilizado Atlas.ti versión 8.4. Los resultados demuestran que las categorías más destacadas son Buenas Prácticas, Colaboración y Función. Las dos modalidades, Blog y Simulación-Videoconferencia, han sido consideradas como buenas prácticas con TIC por el profesorado, estudiantes e instituciones. Estas herramientas les han permitido la recuperación de experiencias debido a la retroalimentación que ofrecen y el fomento de la reflexión sobre sus prácticas y las de sus compañeros/as.
Potential Application of Cephalosporins Carried in Organic or Inorganic Nanosystems against Gram-Negative Pathogens
Authors: Bezerra Brenda Maria Silva , Dantas de Mendonça y Araujo Sara Efigênia , Cordeiro de Macêdo Analara , Costa Kammila Martins Nicolau , Sato Mariana Rillo , Oshiro-Junior João Augusto
Published: March 2022

Cephalosporins are β-lactam antibiotics, classified into five generations and extensively
used in clinical practice against infections caused by Gram-negative pathogens,
including Enterobacteriaceae and P. aeruginosa. Commercially, conventional pharmaceutical
forms require high doses to ensure clinical efficacy. Additionally, β-lactam resistance
mechanisms, such as the production of enzymes (called extended-spectrum β-lactamases)
and the low plasma half-life of these antibiotics, have been challenging in clinical
therapy based on the use of cephalosporins. In this context, its incorporation into nanoparticles,
whether organic or inorganic, is an alternative to temporally and spatially control
the drug release and improve its pharmacokinetic and pharmacodynamic limitations. Considering
this, the present review unites the cephalosporins encapsulated into organic and
inorganic nanoparticles against resistant and nonresistant enterobacteria. We divide
cephalosporin generation into subtopics in which we discuss all molecules approved by
regulatory agencies. In addition, changes in the side chains at positions R1 and R2 of the
central structure of cephalosporins for all semisynthetic derivatives developed were discussed
and presented, as the changes in these groups are related to modifications in pharmacological
and pharmacokinetic properties, respectively. Ultimately, we exhibit the advances
and differences in the release profile and in vitro activity of cephalosporins incorporated
in different nanoparticles.
Biological Activities and Therapeutic Potential of Irigenin on Gastric,
Lung, Prostate, Breast, and Endometrial Cancer: Pharmacological and
Analytical Aspects
Authors: Patel Dinesh Kumar
Published: March 2022

Background:

Herbal medicine has been in used in the medicine for thousands of years due to its multiple pharmacological activities against different types of human disorders. Biological effectiveness and pharmacological activities of herbal medicines is mainly due to the presence of different kinds of phytoconstituents commonly called secondary metabolites. Isoflavones are naturally present in different types of plants and food materials and most of these phytocompounds have a wide range of biological activities including anti-tumor, anti-proliferative, anti-inflammatory, anti-microbial, anti-viral, anti-oxidant, and estrogen-like activities.

Methods:

Irigenin isolated from the rhizome of Belamcanda chinensis and the genus Iris showed a wide range of biological activities. Due to huge biological potential and the therapeutic benefit of irigenin in the medicine, here in the present investigation, scientific research data of irigenin has been collected from different literature sources (Science Direct, Google Scholar, PubMed, Google) and analyzed in order to know the therapeutic potential of irigenin in the medicine. Pharmacological activities scientific data of irigenin have been collected from Science Direct, Google Scholar, PubMed, Google databases and analyzed in the present work. Importance of different analytical techniques for isolation and separation of irigenin has been also discussed and presented in the present work.

Results:

Scientific data analysis of different research work revealed the biological importance of irigenin in the medicine. Irigenin is an O-methylated isoflavone found to be present in the Belamcanda chinensis and genus Iris. Scientific data analysis revealed the biological importance of irigenin against gastric cancer, lung carcinoma, prostate cancer, breast cancer, endometrial cancer, diabetic complications, inflammation and cardiac injury. Analytical data of irigenin revealed the importance of analytical techniques for the separation, isolation and identification of irigenin from various biological samples.

Conclusion:

Scientific data analysis of different research works revealed the biological importance and therapeutic potential of irigenin in the medicine.
Therapeutic Effects of Modified Tempeh on Glycemic Control and Gut
Microbiota Diversity in Diabetic Rats
Authors: Kusuma Rio Jati , Widada Jaka , Huriyati Emy , Julia Madarina
Published: March 2022

Background:

The role of the gut microbiota in improving glycemic control in diabetic
patients is gaining attention. Tempeh is a fermented soy food from Indonesia that has antidiabetic
and antidysbiotic effects. Interestingly, modification of tempeh processing by adding lactic acid
bacteria has been reported to enhance the antidiabetic effect of tempeh.

Aim:

This study aimed to evaluate the effects of modified tempeh on serum glucose, insulin,
and gut microbiota diversity of diabetic rats.

Methods:

Modified tempeh was developed by adding lactic acid bacteria from fermented cassava
during tempeh processing. Diabetes was induced by injection of streptozotocin nicotinamide.
Normal tempeh or modified tempeh was added to the diet and replaced 15% or 30% of casein.
Serum glucose and insulin were analyzed before and after 30 days of intervention. At the end of
the experiment, the appendix was sampled for gut microbiota analysis.

Result:

Modified tempeh has a significantly higher number of lactic acid bacteria (9.99 ± 0.09
versus 7.74 ± 0.07 log CFU, p < 0.001) compared to normal tempeh. There was a significant difference
(p < 0.01) in serum glucose and insulin after treatment. Both tempeh supplements increased
the diversity of the gut microbiota. Gut microbiota diversity has a strong negative correlation
with delta glucose (r = -0.63, p < 0.001) and delta insulin resistance index (r = -0.54, p =
0.003).

Conclusion:

Modified tempeh has potential therapeutic antidiabetic activity, possibly through
increased diversity of the gut microbiota.
Efficacy, Benefits, and Harms of a Self-management App in a Swedish Trauma-Exposed Community Sample (PTSD Coach): Randomized Controlled Trial
Authors: Hensler Ida , Sveen Josefin , Cernvall Martin , Arnberg Filip K
Published: March 2022

Background

Self-guided interventions may complement and overcome obstacles to in-person treatment options. The efficacy of app interventions targeting posttraumatic stress disorder (PTSD) is unclear, and results from previous studies on PTSD Coach—an app for managing trauma-related distress—are inconsistent.

Objective

This study investigates whether access to the Swedish version of the PTSD Coach affects posttraumatic stress, depressive, and somatic symptoms. In addition, we aim to assess the perceived helpfulness, satisfaction, negative effects, response, and remission related to PTSD Coach.

Methods

Adults who had experienced potentially traumatic events in the past 2 years were randomized (1:1) to have access to PTSD Coach (n=89) or be on the waitlist (n=90). We assessed clinical characteristics at baseline (semistructured interviews and self-rating scales) and after 3 months (self-rating scales). We analyzed the data in R software using linear mixed effects models, chi-square tests, and Fisher exact test.

Results

Intention-to-treat analyses indicated that access to PTSD Coach decreased posttraumatic stress and depressive symptoms but not somatic symptoms. More participants who had access to PTSD Coach responded with clinically significant improvement and fewer instances of probable PTSD after 3 months compared with waitlist controls. Overall, participants found that PTSD Coach was slightly to moderately helpful and moderately satisfactory. Half of the intervention group (36/71, 51%) reported at least one negative reaction related to using PTSD Coach (eg, disappointment with the app or its results, arousal of stress, or distressing memories).

Conclusions

Using PTSD Coach may trigger symptoms among a few users; however, most of them perceived PTSD Coach as helpful and satisfactory. This study showed that having access to PTSD Coach helped improve psychological trauma-related symptoms. In addition, we have discussed implications for future research and clinical practice.

Trial Registration

ClinicalTrials.gov NCT04094922; https://clinicaltrials.gov/ct2/show/NCT04094922
PG Recommendation
Authors: Sharma Mohit , Baliyan Hritik , Chikara Akhil , Sharma Govind
Published: March 2022

Abstract: When you plan of going to study far away from your comfort zone, one of the first things to do is to book a good place to stay. Booking a PG online can be an overwhelming task with thousands of PG to choose from.Inspired by the importance of these situations, we made a decision to apply our skills on the task of recommending PG to Students. We used a hotel recommendation dataset, which provided us with a variety of features that helpedus gain a deeper understanding of the process that makes a user choose certain PG. The aim of this PG recommendation project is to predict and recommend best PG to a user that he/she is more likely to book from the given hundred distinct choices.
Didática da história e tecnologias digitais: as narrativas históricas no YouTube Edu
Authors: Rodrigues Junior Osvaldo
Published: March 2022

Este artigo apresenta resultados de pesquisa que teve como objetivo analisar as narrativas históricas disponibilizadas na plataforma YouTube Edu. Criada em 2013 por iniciativa da Google, em parceria com a Fundação Lemann, a plataforma tem como objetivo reunir vídeos educacionais produzidos por professores. Partindo da concepção de Didática da História como campo de estudo das formas com que a consciência histórica se manifesta na sociedade, foram analisados vídeos dos canais Descomplica e Nerdologia. Os resultados permitem observar a multiplicidade de narrativas históricas presentes no YouTube e a sua relação com a finalidade dos canais analisados.
On the Question of the Ideology of the Eurasian Economic Union
Authors: Glazyev S. Yu. , Kefeli I. F.
Published: March 2022

Aim. To update attention on the need to form the ideology of Eurasian integration, expressing not only economic, but also common socio-political and spiritual interests and a vision of the common future of the EAEU.

Tasks. To reveal the essence of the ideology of Eurasian nationalism, the value content of the concept of the Community of the Common Destiny of Humanity and the possibility of their complementary integrity in the ethno-political continuum of Greater Eurasia.

Methods. Civilizational and concrete-historical approaches, as well as axiological discourse regarding the ideological choice in a multi-ethnic community.

Results. The enduring value of the concepts of Eurasian nationalism, inverted in relation to modernity, and the Community of the Common Destiny of Mankind is substantiated. The main directions of the search for theoretical and axiological foundations are determined.

Conclusion. On the agenda of Eurasian integration is the search for conditions for the rejection of libertarian ideology, serving the interests of international capital, and not peoples with a common historical destiny. It is necessary to search for its own kind of social-conservative synthesis, which combines the restoration of socialist ideology with the spiritual traditions of the Eurasian peoples.
Pengembangan Modul Pembelajaran Biologi Berbasis Inkuiri Terbimbing pada Materi Ekosistem untuk Siswa Kelas X SMAN 2 Menggala
Authors: Restiana Vienna , Suhendi Suhendi , Yudiyanto Yudiyanto , Hakim Nasrul
Published: March 2022

The purpose of this study was to determine the characteristics of the guided inquiry-based biology learning module on ecosystem material for class X high school students, to determine the feasibility of the guided inquiry-based biology learning module on ecosystem material for class X high school students, and to find out the responses from biology teachers and students. In terms of the results of the questionnaire assessment. This study uses the Research and Development method developed by Sugiyono to develop printed learning media in the form of modules. Before testing the product, the finished initial product is then validated by experts. The final result of the material expert got a percentage of 86% with the "Very Eligible" criteria, and media expert validation got a percentage of 92% with the "Very Eligible" criteria. After that, a limited-scale trial was conducted, the results of the biology teacher's response were 95.41% and from 10 students the percentage was 90.50%. In conclusion, the module developed has the criteria of "Very Good" and is suitable for use in the learning.
Key words: Learning Module, Guided Inquiry, Ecosystem
 
Abstrak. Tujuan penelitian ini adalah untuk mengetahui karakteristik modul pembelajaran biologi berbasis inkuiri terbimbing pada materi ekosistem untuk siswa kelas X SMA, untuk mengetahui kelayakan modul pembelajaran biologi berbasis inkuiri terbimbing pada materi ekosistem untuk siswa kelas X SMA, dan untuk mengetahui tanggapan dari guru biologi dan peserta didik ditinjau dari hasil penilaian angket. Penelitian ini menggunakan metode Research and Development yang dikembangkan oleh Sugiyono untuk mengembangkan media pembelajaran cetak dalam bentuk modul. Sebelum menguji produk, produk awal yang telah selesai kemudian divalidasi oleh para ahli. Hasil akhir dari ahli materi mendapatkan presentase 86% dengan kriteria “Sangat Layak”, dan validasi ahli media mendapatkan presentase 92% dengan kriteria “Sangat Layak”. Setelah itu dilakukan uji coba skala terbatas, hasil respon guru biologi 95,41% dan dari 10 siswa persentasenya 90,50%. Kesimpulannya, modul yang dikembangkan memiliki kriteria “Sangat Baik”  dan layak untuk digunakan dalam proses pembelajaran.
Kata kunci: Modul Pembelajaran, Inkuiri Terbimbing, Ekosistem
The organs of the date palm: an ecologically clean raw material for human use in the Algerian Sahara.
Authors:   Universite de Ghardaia, Ouargla, Algeria, Siboukeur Abdellah , Sebihi Abdelhafid ,   Maitre assitant, Saharan bio-resources laboratory.,Univ. kasdi Merbah, Ouargla, Algérie, Senoussi Abdelhakim ,   Profesor, Saharan bio-resources laboratory.,Univ. kasdi Merbah, Ouargla, Algérie, Babahani Souad ,   Saharan bio-resources laboratory.,Univ. kasdi Merbah, Ouargla, Algérie
Published: March 2022

The region of Ouargla, located in the south-east of Algeria, is one of the main dateproducing areas. Date palm is a source for and various products. It produces, every year, dates that are, traditionally, considered as a basic food for the local community as well as a commercial product. This phoenicicole biomass is also used for different purposes (building, heating, fodder, cooking, agriculture, therapy and cosmetics). This study aims to value the products of oasis land, through date palms products, and to endeavor to highlight the economic importance of these products and their uses in order to consider strategies for developing this patrimony. Nine sites are retained for investigation. They represent localities which are notorious for having kept, so far, knowledge and know-how in the use of dates as well as other organs of the date palm, and where these products are likely integrated in the socio-economic life of their populations. The aim is to determine the raw material of production, the uses of the date palm organs, dates’ fields of usage and the importance of the products derived from the phoenicicol mass in the commercial environment. We noticed that the organs of the date palm, which are considered as a raw material, are available in a considerable tonnage. An important range of sub-products is derived from the date palm. It is used in basket-making (utensils, cabinetmaking, and garden-work material) and as a livestock aliment with high economic revenue. Besides, these organs (palms, spines, petiole, seeds, sap, pollen…) are used in making medicinal and cosmetic products. All in all, these products are biological and start making their place in modern trade, which might give a new dynamic to the local and national economy.
The Biodiversity of Pelagic Fish Caught by Purse Seines in Tripoli, northern Lebanese Coast.
Authors:   National Council for Scientific Research in Lebanon, Batroun, Lebanon, Jemaa Sharif , Lteif Myriam ,   National Council for Scientific Research in Lebanon, Batroun, Lebanon, Khalaf Gaby ,   National Council for Scientific Research in Lebanon, Batroun, Lebanon, Fakhri Milad ,   National Council for Scientific Research in Lebanon, Batroun, Lebanon
Published: March 2022

This study highlights for the first time the biodiversity of fish assemblages in purse seine fisheries in the Tripoli region, north of Lebanon. A total of 925 specimens from 17 species of 11 families were identified and studied from the monthly samples collected between April and November 2017. The catch was dominated by 3 families (Clupeidae, Engraulidae and Dussumieriidae) in varying abundances. Seasonal variation of abundance was detected with Sardina pilchardus (32%) in Spring, Engraulis encrasicolus (56%) in Summer and Sardinella maderensis (58%) in Fall. Weight length relationships (W=aLb ) were estimated for the most abundant species, and they show a positive allometry for Engraulis encrasicolus (b=3.276), as well as, Herklotsichthys punctatus (b=3.477), and a negative allometry for Sardinella aurita (b=2.886). Seasonal diversity was evaluated and the highest diversity was observed during the spring season following Shannon Index (H’=1.44), Pielou evenness (J’=0.74) and Simpson Index (1-D=0.74). The Shannon Index differed from one season to another reaching the highest value in Spring. Despite the progress in studying diversity and abundance, variation in pelagic fish catches is evident in Lebanese sea water, where a management plan for purse seine fisheries should be applied under the framework of ecosystem approach to fisheries.
The effects of various tillage systems on soil structure and root system development in wheat (Triticum durum Desf.).
Authors:   Laboratory of water control in agriculture. Department of Agricultural Engineering. National Higher School of Agronomy (ENSA), ES1603, El Harrach, Algiers, Algeria., Echcherki Smain , Amine Feddal Mohamed ,   Laboratory of water control in agriculture. Department of Agricultural Engineering. National Higher School of Agronomy (ENSA), ES1603, El Harrach, Algiers, Algeria., Labad Ryma ,   Laboratory of water control in agriculture. Department of Agricultural Engineering. National Higher School of Agronomy (ENSA), ES1603, El Harrach, Algiers, Algeria.
Published: March 2022

The objective of our study is to compare the effect of agricultural soil preparation on the physicomechanical properties of the soil and the consequences on the root system of wheat. To achieve these goals, three technical tillage chains (chain 1: deep tillage, chain 2: agronomic tillage, chain 3: no-tillage), were compared in the same pedoclimatic and historical situations. The experiment was carried out on silty clay soil in the experimental station of the National Higher School of Agronomy (Algiers). The results show that the techniques affect soil’s proprieties and the root system differently. The agronomic tillage has the best favorable effect on soil humidity, Bulk density, and soil Penetration resistance (11,1%; 50%; 0.6 MPa) (p-value :0,0001). Concerning the roots, the density, the diameter, and length were better when the soil is deeply tilled, non-tilled, or agronomical tilled, respectively (CH1:3.37 g/dm³; CH3:1.38 mm; CH2:12.23 cm). Examining all the results of this study has shown how tillage is reflected in and contributes to the farmer's orientation towards the right choice to optimize and conserve soil and water at the same time. Thus, under this study's experimental conditions, the technical itinerary of tillage with agronomic ploughing seems to be an alternative to conventional ploughing. This method of tillage is easily feasible from a technical point of view. Besides, it can generate economic gains compared to ploughing. However, its impacts on the soil and crop yields need to be studied in the longer term.
Behaviors related to chronic non-communicable diseases development in university students.
Authors: Lerma Castaño Piedad Rocío , Jaimes Guerrero Carlos Alberto , Mantilla Toloza Sonia Carolina , Castro Betancourt Dolly , Tamayo Perdomo Gladys , Antolínez Álvarez Martha Florelia , Alvarado Rozo Omar Alberto
Published: March 2022

Introduction: The scientific literature has reported the trend of the impact of non- communicable diseases on public health, and therefore, the investment of resources that interfere in the development of a country.

Objective: To identify the presence of specific behaviors associated with the development of chronic non-communicable diseases in university students, since they are a susceptible population to the modification of these tendencies.

Materials and methods: This cross-sectional descriptive study was carried out on a 375 university students’ sample. The stepwise method was applied to identify risk factors associated with the development of chronic non-communicable diseases. Descriptive statistics and bivariate analysis were used to determine possible relationships between the variables studied.

Results: The prevalence of tobacco use was 7.5%, frequent alcohol consumption was 91.5%, fruit intake was 96.3%, and vegetable intake was 95%. Additionally, only 48% of the sample practiced intense physical activity. Also, a relationship among the male sex, with the consumption of tobacco and the practice of low physical activity was found.

Conclusions: Behaviors associated with chronic non-communicable diseases such as alcohol and tobacco consumption were identified in university students.
Use of the cardiac ultrasound at emergency room for general physicians
Authors: Kataoka Hiromitsu , Mendoza Ortiz Luis Fernando , Salazar Restrepo Daniela
Published: March 2022

Ultrasonography at the Emergency Room (ER) becomes a useful tool for emergency doctors, especially when they need to attend to diseases that require faster treatment. This is an ideal non-invasive exam which is cheap and can be implemented in real time to obtain instantly the missing information. The quick recognition of the causes of the cardiac instability will lead to better results for conditions such as cardio-pulmonary resuscitation (CPR). The aim of this article is to show the usefulness of cardiac ultrasonography and to briefly teach general practitioners at ER about it.
A Chromosome-level genome assembly of the striped catfish (Pangasianodon hypophthalmus) reveals molecular mechanisms for its high-fat trait
Authors: Gao Zijian , You Xinxin , Zhang Xinhui , Chen Jieming , Tengfei Xu , Huang Yu , Lin Xueqiang , Xu Junmin , Bian Chao , Shi Qiong
Published: March 2022

Striped catfish (Pangasianodon hypophthalmus), belonging to the
Pangasiidae family, has become an economically important fish with wide
cultivation in Southeast Asia. Owing to the high-fat trait, it is always
considered as an oily fish. In our present study, a high-quality genome
assembly of the striped catfish was generated by integration of short
reads from an Illumina Hiseq Xten platform, long reads from a Nanopore
platform, and Hi-C sequencing data. This assembled genome is 731.7 Mb in
length, with a scaffold N50 of 29.5 Mb and anchoring on 30 chromosomes.
A total 18,895 protein-coding genes were predicted, among them 98.46%
were functionally annotated. Interestingly, we identified a tandem
triplication of fatty acid binding protein 1 gene (fabp1; thereby named
as fabp1-1, fabp1-2 and fabp1-3 respectively), which may be critical for
molecular regulation of the high-fat trait in the striped catfish.
Compared with Fabp1-1 (similar to the conserved Fabp1 in various
vertebrate species), the R126T mutation may potentially affect the fatty
acid binding capacity of the Fabp1 isotypes 2 and 3. In summary, we
report a high-quality chromosome-level genome assembly of the striped
catfish, which provides a valuable genetic resource for biomedical
studies on the high-fat trait, and laying a foundation for practical
aquaculture and molecular breeding of this international teleost
species.
Integrability, multiple-solitons, breathers and lumps for a new (3+1)-dimensional Kadomtsev–Petviashvili equation
Authors: Ma Yu-Lan , wazwaz abdul-majid , Li Bang-Qing
Published: March 2022

In this paper, a new (3+1)-dimensional integrable
Kadomtsev–Petviashvili equation is developed. Its integrability is
verified by the Painlev\’e analysis. The bilinear form,
multiple-soliton, breather and lump solutions are obtained via using the
Hirota bilinear method. Furthermore, the abundant dynamical behaviors
for these solutions are discovered. It is interesting that there are
splitting and fusing phenomena when the lumps interact.
Paeoniflorin-6′-O-benzene sulfonate inhibits macrophage pyroptosis via TLR4/ NLRP3/ GSDMD signaling pathway in adjuvant arthritis rats
Authors: Xu Li , Wang Han , Yu Qianqian , Ge Jinru , Zhang Xianzheng , Mei Dan , Liang Faqin , Cai Xiaoyu , Zhu Yue , Shu Jinling , Zhang Ling-Ling , Wei Wei
Published: March 2022

Abstract Purpose. To investigate the mechanisms of macrophage pyroptosis
mediated by TLR4/NLRP3/GSDMD signaling pathway in adjuvant arthritis
(AA) rats and the role of Paeoniflorin-6′-O-benzene sulfonate (CP-25).
Experimental Approach. AA model was induced in Wistar rats via complete
Freund’s adjuvant. Normal group, AA model group, CP-25 (50 mg/kg) group
and MTX (0.5 mg/kg) group were included in this experiment. The
co-expression of TLR4 and NLRP3 and membrane expression of GSDMD and
NLRP3 in macrophages were detected by immunofluorescence assay. The
expression of TLR4, the ratio of macrophage pyroptosis and M1/2-type
macrophages were detected by Flow Cytomery. Cell morphology was observed
by scanning electron microscopy. The levels of IL-18 and IL-1β cytokines
in plasma and supernatant of cultured macrophage were detected by ELISA.
The expression of TLR4, MyD88, NLRP3, Caspase-1, ASC and GSDMD in
macrophages was detected by Western Blot. Key Results. Macrophage
pyroptosis was found in AA rats; CP-25 has a therapeutical effect on AA
rats by improving the joint inflammation and reducing the pathological
process of the joints of AA rats; CP-25 can inhibit the pyroptosis of
macrophages by down-regulate the expression of TLR4, MyD88, NLRP3,
Caspase-1, ASC and GSDMD of macrophages in vivo; CP-25 inhibits LPS and
ATP-induced macrophages pyroptosis by inhibiting the activation of
TLR4/NLRP3/GSDMD signaling pathway in vitro. Conclusion and
Implications. Macrophage pyroptosis was mediated through
TLR4/NLRP3/GSDMD signaling pathway, and CP-25 can regulate macrophage
pyroptosis by inhibiting TLR4/NLRP3/GSDMD signaling pathway, thereby
improving synovitis in AA rats.
Respiration, not photosynthesis, determines yield loss caused by global warming
Authors: Li Guangyan , Chen TingTing , Feng Baohua , Peng Shaobing , Tao Longxing , Fu Guanfu
Published: March 2022

Photosynthesis is an important biochemical reaction that provides food
and oxygen to maintain aerobic life on earth. Recently, increasing
photosynthesis has been revisited as an approach for reducing crop yield
losses caused by global warming. Here, we argue that respiration, rather
than photosynthesis, may be the primary contributor to yield losses in a
warming climate. Moderate high temperature stress causes little damage
to photosynthesis but significantly increases respiration rate. In this
case, energy production efficiency is enhanced by high temperature, but
most of this energy is allocated to maintenance respiration, resulting
in an overall decrease in energy utilization efficiency. Engineering
crops with low respiration and high energy utilization efficiency could
therefore effectively solve the world food crisis as global warming
increases.
Joint evolution of mycorrhizal type, pollination, and seed dispersal mode in trees
Authors: Yamawo Akira , Ohno Misuzu
Published: March 2022

Trees form the bases of terrestrial ecosystems; however, the origin of
the diversity of trees within these systems is a major question in
evolutionary biology and ecology. Mycorrhizal type, pollination, and
seed dispersal mode are key drivers of tree diversity. However, because
they are usually studied in isolation, their evolutionary relationships
are poorly understood. Here, we show the evolutionary relationships
among mycorrhizal type, seed dispersal mode, and pollination mode in 821
tree species. We found evidence of the joint evolution of all three
features. Most arbuscular mycorrhizal-associated trees had
endozoochorous seed dispersal and biotic pollination, whereas most
ectomycorrhizal-associated trees had anemochorous seed dispersal and
wind pollination. Overall, mycorrhizal type, seed dispersal mode, and
pollination mode were linked, emphasising the importance of the joint
study of these traits to understand tree diversity. Plants have lived
closely with AM fungi for over 400 million years, and later evolved
mutualistic interactions that promote plant diversification. This raises
a novel question over how mycorrhizal symbiosis drives the evolution of
seed and pollination mutualism, and how it contributes to plant
diversification. Here, we provide a comprehensive reliable scaffold for
understanding not only tree evolution but also the evolution and
mechanisms of forest ecosystems.
Development of an antigen Enzyme-Lynked AptaSorbent Assay (ELASA) for the detection of Swine Influenza virus in field samples
Authors: Pino Cristina Aira , Mingo José Ignacio Klett , Ruiz Tamara , Sacristán Ana García , Martín-Valls Gerard , GÓMEZ-LAGUNA JAIME , Rueda Paloma , González Víctor Manuel , Rodriguez Maria Jose , López Lissett
Published: March 2022

Influenza viruses are highly variable pathogens that infect a wide range
of mammalian and avian species. According to the internal conserved
proteins (nucleoprotein: NP, and matrix proteins: M), these viruses are
classified into type A, B, C, and D. Influenza A virus in swine is of
significant importance to the industry since it is responsible of
endemic infections that lead to high economic loses derived from poor
weight gain, reproductive disorders, and the role it plays in Porcine
Respiratory Disease Complex (PRDC). To the date, swine influenza virus
(SIV) diagnosis keeps on being based in complex and expensive
technologies such as RT-qPCR. In this study, we aimed to improved actual
tools by the implementation of new bioreceptors molecules; aptamers.
First, three different aptamers have been selected using the recombinant
NP of Influenza A virus expressed in insect cells, as target. Then,
these molecules have been used for the development of an Enzyme-Linked
AptaSorbent Assay (ELASA) in combination with specific monoclonal
antibodies for Influenza A detection. A total of 171 field samples
(nasal swabs) have been evaluated with the newly developed assay
obtaining a 79.7 % and 98.1 % sensitivity and specificity
respectively, using real time RT-PCR as standard assay. These results
suggest that the assay is a promising method that could be used for
Influenza A detection in analysis laboratories facilitating surveillance
labours.