bluuebunny/crossref_metadata_2025 huggingface dataset:
Regional Delimitation of PM2.5 Pollution Using Spatial Cluster for Monitoring Sites: A Case Study of Xianyang, China
Authors: Zhang Bo , Zhou Fang , Song Guojun
Published: September 2020

Fine particulate matter (PM2.5) pollution has been a major concerning issue in China, and many cities have developed emergency plans for heavy air pollution. The aim of this study is to delimitate PM2.5 pollution regions of Xianyang, which is very important to the regional environmental prevention and control. The result showed that PM2.5 air pollution had significant cross-administrative characteristics in Xianyang. Using spatial clustering algorithm under adjacent matrix constrain, this study classified the air quality monitoring sites into two clusters. For each monitoring site, we generated Voronoi polygons and ultimately Xianyang was delimitated into two regions, south and north. The air pollution of the southern region was more serious with 64 days of heavy and severe pollution since 2018, while the northern region had only 10 days. The southern region consisted of four complete administrative districts and parts of three administrative districts. While the northern region consisted of six complete administrative districts and parts of three administrative districts. Visualization of the spatiotemporal characteristics of the PM2.5 air pollution in the two regions further illustrated the significant difference. We suggest when heavy pollution happens, the two regions should be considered separately. If the southern region is heavily polluted while the northern region not, only the southern region needs to implement the emergency plan to minimize the damage to society and economy. Seventy-five percent of the city area, 2.3 million people, 59% of schools, and 43% of GDP would not be impacted if air pollution was controlled by region separately. The sensitive analysis shows that clustering result is robust against different pollution degree and missing values.
Quantitative Lipidomic Analysis of Osteosarcoma Cell-Derived Products by UHPLC-MS/MS
Authors: Casati Sara , Giannasi Chiara , Minoli Mauro , Niada Stefania , Ravelli Alessandro , Angeli Ilaria , Mergenthaler Veronica , Ottria Roberta , Ciuffreda Pierangela , Orioli Marica , Brini Anna T.
Published: September 2020

Changes in lipid metabolism are involved in several pathological conditions, such as cancer. Among lipids, eicosanoids are potent inflammatory mediators, synthesized from polyunsaturated fatty acids (PUFAs), which coexist with other lipid-derived ones, including endocannabinoids (ECs) and N-acylethanolamides (NAEs). In this work, a bioanalytical assay for 12 PUFAs/eicosanoids and 20 ECs/NAEs in cell culture medium and human biofluids was validated over a linear range of 0.1–2.5 ng/mL. A fast pretreatment method consisting of protein precipitation with acetonitrile followed by a double step liquid–liquid extraction was developed. The final extracts were injected onto a Kinetex ultra-high-performance liquid chromatography (UHPLC) XB-C18 column with a gradient elution of 0.1% formic acid in water and methanol/acetonitrile (5:1; v/v) mobile phase. Chromatographic separation was followed by detection with a triple-quadrupole mass spectrometer operating both in positive and negative ion-mode. A full validation was carried out in a small amount of cell culture medium and then applied to osteosarcoma cell-derived products. To the best of our knowledge, this is the first lipid profiling of bone tumor cell lines (SaOS-2 and MG-63) and their secretome. Our method was also partially validated in other biological matrices, such as serum and urine, ensuring its broad applicability as a powerful tool for lipidomic translational research.
Mesenchymal Stem Cells as a Promising Cell Source for Integration in Novel In Vitro Models
Authors: Afflerbach Ann-Kristin , Kiri Mark D. , Detinis Tahir , Maoz Ben M.
Published: September 2020

The human-relevance of an in vitro model is dependent on two main factors—(i) an appropriate human cell source and (ii) a modeling platform that recapitulates human in vivo conditions. Recent years have brought substantial advancements in both these aspects. In particular, mesenchymal stem cells (MSCs) have emerged as a promising cell source, as these cells can differentiate into multiple cell types, yet do not raise the ethical and practical concerns associated with other types of stem cells. In turn, advanced bioengineered in vitro models such as microfluidics, Organs-on-a-Chip, scaffolds, bioprinting and organoids are bringing researchers ever closer to mimicking complex in vivo environments, thereby overcoming some of the limitations of traditional 2D cell cultures. This review covers each of these advancements separately and discusses how the integration of MSCs into novel in vitro platforms may contribute enormously to clinical and fundamental research.
Effects of New NSAID-CAI Hybrid Compounds in Inflammation and Lung Fibrosis
Authors: Lucarini Laura , Durante Mariaconcetta , Sgambellone Silvia , Lanzi Cecilia , Bigagli Elisabetta , Akgul Ozlem , Masini Emanuela , Supuran Claudiu T. , Carta Fabrizio
Published: September 2020

Pulmonary fibrosis is a severe lung disease with progressive worsening of dyspnea, characterized by chronic inflammation and remodeling of lung parenchyma. Carbonic anhydrases are a family of zinc-metallo-enzymes that catalyze the reversible interconversion of carbon-dioxide and water to bicarbonate and protons. Carbonic Anhydrase Inhibitor (CAI) exhibited anti-inflammatory effects in animals with permanent-middle-cerebral artery occlusion, arthritis and neuropathic pain. The pharmacological profile of a new class of hybrid compounds constituted by a CAI connected to a Nonsteroidal-Anti-Inflammatory Drug (NSAID) was studied in the modulation of inflammation and fibrosis. In-vitro tests were performed to assess their effects on cyclo-oxygenase enzyme (COX)-1 and COX-2, namely inhibition of platelet aggregation and thromboxane B2 production in the human-platelet-rich plasma, and reduction of Prostaglandin-E2 production in lipopolysaccharide-treated-RAW-264.7 macrophage cell line. The activity of compound 3, one of the most active, was studied in a model of bleomycin-induced lung fibrosis in C57BL/6 mice. The hybrid compounds showed a higher potency in inhibiting PGE2 production, but not in modifying the platelet aggregation and the TXB2 production in comparison to the reference molecules, indicating an increased activity in COX-2 inhibition. In the in-vivo murine model, the compound 3 was more effective in decreasing inflammation, lung stiffness and oxidative stress in comparison to the reference drugs given alone or in association. In conclusion, these CAI-NSAID hybrid compounds are promising new anti-inflammatory drugs for the treatment of lung chronic inflammatory diseases.
Effects of the Delta Opioid Receptor Agonist DADLE in a Novel Hypoxia-Reoxygenation Model on Human and Rat-Engineered Heart Tissue: A Pilot Study
Authors: Funcke Sandra , Werner Tessa R. , Hein Marc , Ulmer Bärbel M. , Hansen Arne , Eschenhagen Thomas , Hirt Marc N.
Published: September 2020

Intermittent hypoxia and various pharmacological compounds protect the heart from ischemia reperfusion injury in experimental approaches, but the translation into clinical trials has largely failed. One reason may lie in species differences and the lack of suitable human in vitro models to test for ischemia/reperfusion. We aimed to develop a novel hypoxia-reoxygenation model based on three-dimensional, spontaneously beating and work performing engineered heart tissue (EHT) from rat and human cardiomyocytes. Contractile force, the most important cardiac performance parameter, served as an integrated outcome measure. EHTs from neonatal rat cardiomyocytes were subjected to 90 min of hypoxia which led to cardiomyocyte apoptosis as revealed by caspase 3-staining, increased troponin I release (time control vs. 24 h after hypoxia: cTnI 2.7 vs. 6.3 ng/mL, ** p = 0.002) and decreased contractile force (64 ± 6% of baseline) in the long-term follow-up. The detrimental effects were attenuated by preceding the long-term hypoxia with three cycles of 10 min hypoxia (i.e., hypoxic preconditioning). Similarly, [d-Ala2, d-Leu5]-enkephalin (DADLE) reduced the effect of hypoxia on force (recovery to 78 ± 5% of baseline with DADLE preconditioning vs. 57 ± 5% without, p = 0.012), apoptosis and cardiomyocyte stress. Human EHTs presented a comparable hypoxia-induced reduction in force (55 ± 5% of baseline), but DADLE failed to precondition them, likely due to the absence of δ-opioid receptors. In summary, this hypoxia-reoxygenation in vitro model displays cellular damage and the decline of contractile function after hypoxia allows the investigation of preconditioning strategies and will therefore help us to understand the discrepancy between successful conditioning in vitro experiments and its failure in clinical trials.
Dynamic mRNP Remodeling in Response to Internal and External Stimuli
Authors: Zarnack Kathi , Balasubramanian Sureshkumar , Gantier Michael P. , Kunetsky Vladislav , Kracht Michael , Schmitz M. Lienhard , Sträßer Katja
Published: September 2020

Signal transduction and the regulation of gene expression are fundamental processes in every cell. RNA-binding proteins (RBPs) play a key role in the post-transcriptional modulation of gene expression in response to both internal and external stimuli. However, how signaling pathways regulate the assembly of RBPs with mRNAs remains largely unknown. Here, we summarize observations showing that the formation and composition of messenger ribonucleoprotein particles (mRNPs) is dynamically remodeled in space and time by specific signaling cascades and the resulting post-translational modifications. The integration of signaling events with gene expression is key to the rapid adaptation of cells to environmental changes and stress. Only a combined approach analyzing the signal transduction pathways and the changes in post-transcriptional gene expression they cause will unravel the mechanisms coordinating these important cellular processes.
Effect of Brain Lesions on Voluntary Cough in Patients with Supratentorial Stroke: An Observational Study
Authors: Lee Kyoung Bo , Lim Seong Hoon , Park Geun-Young , Im Sun
Published: September 2020

Patients with stroke are known to manifest a decreased cough force, which is associated with an increased risk of aspiration. Specific brain lesions have been linked to impaired reflexive coughing. However, few studies have investigated whether specific stroke lesions are associated with impaired voluntary cough. Here, we studied the effects of stroke lesions on voluntary cough using voxel-based lesion-symptom mapping (VLSM). In this retrospective cross-sectional study, the peak cough flow was measured in patients who complained of weak cough (n = 39) after supratentorial lesions. Brain lesions were visualized via magnetic resonance imaging (MRI) at the onset of stroke. These lesions were studied using VLSM. The VLSM method with non-parametric mapping revealed that lesions in the sub-gyral frontal lobe and superior longitudinal and posterior corona radiata were associated with a weak cough flow. In addition, lesions in the inferior parietal and temporal lobes and both the superior and mid-temporal gyrus were associated with a weak peak cough flow during voluntary coughing. This study identified several brain lesions underlying impaired voluntary cough. The results might be useful in predicting those at risk of poor cough function and may improve the prognosis of patients at increased risk of respiratory complications after a stroke.
Levetiracetam Reduced the Basal Excitability of the Dentate Gyrus without Restoring Impaired Synaptic Plasticity in Rats with Temporal Lobe Epilepsy
Authors: González-H Guillermo , Contreras-García Itzel Jatziri , Sánchez-Huerta Karla , Queiroz Claudio M. T. , Gallardo Gudiño Luis Ricardo , Mendoza-Torreblanca Julieta G. , Zamudio Sergio R.
Published: September 2020

Temporal lobe epilepsy (TLE), the most common type of focal epilepsy, affects learning and memory; these effects are thought to emerge from changes in synaptic plasticity. Levetiracetam (LEV) is a widely used antiepileptic drug that is also associated with the reversal of cognitive dysfunction. The long-lasting effect of LEV treatment and its participation in synaptic plasticity have not been explored in early chronic epilepsy. Therefore, through the measurement of evoked field potentials, this study aimed to comprehensively identify the alterations in the excitability and the short-term (depression/facilitation) and long-term synaptic plasticity (long-term potentiation, LTP) of the dentate gyrus of the hippocampus in a lithium–pilocarpine rat model of TLE, as well as their possible restoration by LEV (1 week; 300 mg/kg/day). TLE increased the population spike (PS) amplitude (input/output curve); interestingly, LEV treatment partially reduced this hyperexcitability. Furthermore, TLE augmented synaptic depression, suppressed paired-pulse facilitation, and reduced PS-LTP; however, LEV did not alleviate such alterations. Conversely, the excitatory postsynaptic potential (EPSP)-LTP of TLE rats was comparable to that of control rats and was decreased by LEV. LEV caused a long-lasting attenuation of basal hyperexcitability but did not restore impaired synaptic plasticity in the early chronic phase of TLE.
Targeted Therapies for Epstein-Barr Virus-Associated Lymphomas
Authors: Pei Yonggang , Wong Josiah H. Y. , Robertson Erle S.
Published: September 2020

The Epstein-Barr virus (EBV) is the first human tumor virus identified that can transform quiescent B lymphocytes into lymphoblastoid cell lines (LCLs) in vitro. EBV can establish asymptomatic life-long persistence and is associated with multiple human malignancies, including non-Hodgkin lymphoma and Hodgkin lymphoma, as well as infectious mononucleosis. Although EBV-associated lymphomagenesis has been investigated for over 50 years, viral-mediated transformation is not completely understood, and the development of EBV-specific therapeutic strategies to treat the associated cancers is still a major challenge. However, the rapid development of several novel therapies offers exciting possibilities to target EBV-induced lymphomas. This review highlights targeted therapies with potential for treating EBV-associated lymphomas, including small molecule inhibitors, immunotherapy, cell therapy, preventative and therapeutic vaccines, and other potent approaches, which are novel strategies for controlling, preventing, and treating these viral-induced malignances.
Improved Progression-Free Long-Term Survival of a Nation-Wide Patient Population with Metastatic Melanoma
Authors: Soerensen Anne Vest , Ellebaek Eva , Bastholt Lars , Schmidt Henrik , Donia Marco , Svane Inge Marie
Published: September 2020

Approval of immune checkpoint-inhibitors (ICIs) and BRAF-inhibitors has revolutionized the treatment of metastatic melanoma. Although these drugs have improved overall survival (OS) in clinical trials, real-world evidence for improved long-term survival is still scarce. Clinical data were extracted from the Danish Metastatic Melanoma database. This nation-wide cohort contains data on all patients who received systemic treatment for metastatic melanoma between 2008 and 2016. Ipilimumab, the first approved ICI, was implemented as standard-of-care in Denmark in 2012. Hence, patients were divided in a pre-ICI (2008–2011) and an ICI (2012–2016) era. Patients were defined as long-term survivors if they were alive 3 years after initiation of systemic therapy. Data from 1754 patients were retrieved. Patients treated in the ICI era had an improved median OS (11.3 months, 95% confidence interval (CI) 10.3–12.3) compared with those in the pre-ICI era (median OS 8.3 months, 95% CI 7.4–9.5, p < 0.0001). A higher proportion of long-term survivors was observed in the ICI era (survivors >3 years increased from 13% to 26% and survivors >5 years increased from 9% to 21%; both p < 0.0001). For long-term survivors, known prognostic factors were equally distributed between the two periods, except that long-term survivors in the pre-ICI era were younger. For long-term survivors, 70% were without progression in the ICI era compared with 43% in the pre-ICI era (p < 0.0001). For all patients, the proportion without progression increased from 5% to 18% between the pre-ICI and the ICI era (p < 0.0001), respectively. Implementation of ICI has led to a significant increase in progression-free, long-term survival for real-life patients with metastatic melanoma.
Phosphorylation of OCT4 Serine 236 Inhibits Germ Cell Tumor Growth by Inducing Differentiation
Authors: Kim Dong Keon , Song Bomin , Han Suji , Jang Hansol , Bae Seung-Hyun , Kim Hee Yeon , Lee Seon-Hyeong , Lee Seungjin , Kim Jong Kwang , Kim Han-Seong , Hong Kyeong-Man , Lee Byung Il , Youn Hong-Duk , Kim Soo-Youl , Kang Sang Won , Jang Hyonchol
Published: September 2020

Octamer-binding transcription factor 4 (Oct4) plays an important role in maintaining pluripotency in embryonic stem cells and is closely related to the malignancies of various cancers. Although posttranslational modifications of Oct4 have been widely studied, most of these have not yet been fully characterized, especially in cancer. In this study, we investigated the role of phosphorylation of serine 236 of OCT4 [OCT4 (S236)] in human germ cell tumors (GCTs). OCT4 was phosphorylated at S236 in a cell cycle-dependent manner in a patient sample and GCT cell lines. The substitution of endogenous OCT4 by a mimic of phosphorylated OCT4 with a serine-to-aspartate mutation at S236 (S236D) resulted in tumor cell differentiation, growth retardation, and inhibition of tumor sphere formation. GCT cells expressing OCT4 S236D instead of endogenous OCT4 were similar to cells with OCT4 depletion at the mRNA transcript level as well as in the phenotype. OCT4 S236D also induced tumor cell differentiation and growth retardation in mouse xenograft experiments. Inhibition of protein phosphatase 1 by chemicals or short hairpin RNAs increased phosphorylation at OCT4 (S236) and resulted in the differentiation of GCTs. These results reveal the role of OCT4 (S236) phosphorylation in GCTs and suggest a new strategy for suppressing OCT4 in cancer.
Precision Medicine: Steps along the Road to Combat Human Cancer
Authors: Nassar Samuel F. , Raddassi Khadir , Ubhi Baljit , Doktorski Joseph , Abulaban Ahmad
Published: September 2020

The diagnosis and treatment of diseases such as cancer is becoming more accurate and specialized with the advent of precision medicine techniques, research and treatments. Reaching down to the cellular and even sub-cellular level, diagnostic tests can pinpoint specific, individual information from each patient, and guide providers to a more accurate plan of treatment. With this advanced knowledge, researchers and providers can better gauge the effectiveness of drugs, radiation, and other therapies, which is bound to lead to a more accurate, if not more positive, prognosis. As precision medicine becomes more established, new techniques, equipment, materials and testing methods will be required. Herein, we will examine the recent innovations in assays, devices and software, along with next generation sequencing in genomics diagnostics which are in use or are being developed for personalized medicine. So as to avoid duplication and produce the fullest possible benefit, all involved must be strongly encouraged to collaborate, across national borders, public and private sectors, science, medicine and academia alike. In this paper we will offer recommendations for tools, research and development, along with ideas for implementation. We plan to begin with discussion of the lessons learned to date, and the current research on pharmacogenomics. Given the steady stream of advances in imaging mass spectrometry and nanoLC-MS/MS, and use of genomic, proteomic and metabolomics biomarkers to distinguish healthy tissue from diseased cells, there is great potential to utilize pharmacogenomics to tailor a drug or drugs to a particular cohort of patients. Such efforts very well may bring increased hope for small groups of non-responders and those who have demonstrated adverse reactions to current treatments.
Breast Cancer Subtypes Underlying EMT-Mediated Catabolic Metabolism
Authors: Cho Eunae , Kim Nam , Yun Jun , Cho Sue , Kim Hyun , Yook Jong
Published: September 2020

Efficient catabolic metabolism of adenosine triphosphate (ATP) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) is essentially required for cancer cell survival, especially in metastatic cancer progression. Epithelial–mesenchymal transition (EMT) plays an important role in metabolic rewiring of cancer cells as well as in phenotypic conversion and therapeutic resistance. Snail (SNAI1), a well-known inducer of cancer EMT, is critical in providing ATP and NADPH via suppression of several gatekeeper genes involving catabolic metabolism, such as phosphofructokinase 1 (PFK1), fructose-1,6-bisphosphatase 1 (FBP1), and acetyl-CoA carboxylase 2 (ACC2). Paradoxically, PFK1 and FBP1 are counter-opposing and rate-limiting reaction enzymes of glycolysis and gluconeogenesis, respectively. In this study, we report a distinct metabolic circuit of catabolic metabolism in breast cancer subtypes. Interestingly, PFKP and FBP1 are inversely correlated in clinical samples, indicating different metabolic subsets of breast cancer. The luminal types of breast cancer consist of the pentose phosphate pathway (PPP) subset by suppression of PFKP while the basal-like subtype (also known as triple negative breast cancer, TNBC) mainly utilizes glycolysis and mitochondrial fatty acid oxidation (FAO) by loss of FBP1 and ACC2. Notably, PPP remains active via upregulation of TIGAR in the FBP1-loss basal-like subset, indicating the importance of PPP in catabolic cancer metabolism. These results indicate different catabolic metabolic circuits and thus therapeutic strategies in breast cancer subsets.
Dynamic High-Sensitivity Quantitation of Procollagen-I by Endogenous CRISPR-Cas9 NanoLuciferase Tagging
Authors: Calverley Ben C. , Kadler Karl E. , Pickard Adam
Published: September 2020

The ability to quantitate a protein of interest temporally and spatially at subcellular resolution in living cells would generate new opportunities for research and drug discovery, but remains a major technical challenge. Here, we describe dynamic, high-sensitivity protein quantitation technique using NanoLuciferase (NLuc) tagging, which is effective across microscopy and multiwell platforms. Using collagen as a test protein, the CRISPR-Cas9-mediated introduction of nluc (encoding NLuc) into the Col1a2 locus enabled the simplification and miniaturisation of procollagen-I (PC-I) quantitation. Collagen was chosen because of the clinical interest in its dysregulation in cardiovascular and musculoskeletal disorders, and in fibrosis, which is a confounding factor in 45% of deaths, including those brought about by cancer. Collagen is also the cargo protein of choice for studying protein secretion because of its unusual shape and size. However, the use of overexpression promoters (which drowns out endogenous regulatory mechanisms) is often needed to achieve good signal/noise ratios in fluorescence microscopy of tagged collagen. We show that endogenous knock-in of NLuc, combined with its high brightness, negates the need to use exogenous promoters, preserves the circadian regulation of collagen synthesis and the responsiveness to TGF-β, and enables time-lapse microscopy of intracellular transport compartments containing procollagen cargo. In conclusion, we demonstrate the utility of CRISPR-Cas9-mediated endogenous NLuc tagging to robustly quantitate extracellular, intracellular, and subcellular protein levels and localisation.
Metabolic Constrains Rule Metastasis Progression
Authors: Roda Niccolo’ , Gambino Valentina , Giorgio Marco
Published: September 2020

Metastasis formation accounts for the majority of tumor-associated deaths and consists of different steps, each of them being characterized by a distinctive adaptive phenotype of the cancer cells. Metabolic reprogramming represents one of the main adaptive phenotypes exploited by cancer cells during all the main steps of tumor and metastatic progression. In particular, the metabolism of cancer cells evolves profoundly through all the main phases of metastasis formation, namely the metastatic dissemination, the metastatic colonization of distant organs, the metastatic dormancy, and ultimately the outgrowth into macroscopic lesions. However, the metabolic reprogramming of metastasizing cancer cells has only recently become the subject of intense study. From a clinical point of view, the latter steps of the metastatic process are very important, because patients often undergo surgical removal of the primary tumor when cancer cells have already left the primary tumor site, even though distant metastases are not clinically detectable yet. In this scenario, to precisely elucidate if and how metabolic reprogramming drives acquisition of cancer-specific adaptive phenotypes might pave the way to new therapeutic strategies by combining chemotherapy with metabolic drugs for better cancer eradication. In this review we discuss the latest evidence that claim the importance of metabolic adaptation for cancer progression.
FAM64A: A Novel Oncogenic Target of Lung Adenocarcinoma Regulated by Both Strands of miR-99a (miR-99a-5p and miR-99a-3p)
Authors: Mizuno Keiko , Tanigawa Kengo , Nohata Nijiro , Misono Shunsuke , Okada Reona , Asai Shunichi , Moriya Shogo , Suetsugu Takayuki , Inoue Hiromasa , Seki Naohiko
Published: September 2020

Lung adenocarcinoma (LUAD) is the most aggressive cancer and the prognosis of these patients is unfavorable. We revealed that the expression levels of both strands of miR-99a (miR-99a-5p and miR-99a-3p) were significantly suppressed in several cancer tissues. Analyses of large The Cancer Genome Atlas (TCGA) datasets showed that reduced miR-99a-5p or miR-99a-3p expression is associated with worse prognoses in LUAD patients (disease-free survival (DFS): p = 0.1264 and 0.0316; overall survival (OS): p = 0.0176 and 0.0756, respectively). Ectopic expression of these miRNAs attenuated LUAD cell proliferation, suggesting their tumor-suppressive roles. Our in silico analysis revealed 23 putative target genes of pre-miR-99a in LUAD cells. Among these targets, high expressions of 19 genes were associated with worse prognoses in LUAD patients (OS: p < 0.05). Notably, FAM64A was regulated by both miR-99a-5p and miR-99a-3p in LUAD cells, and its aberrant expression was significantly associated with poor prognosis in LUAD patients (OS: p = 0.0175; DFS: p = 0.0276). FAM64A knockdown using siRNAs suggested that elevated FAM64A expression contributes to cancer progression. Aberrant FAM64A expression was detected in LUAD tissues by immunostaining. Taken together, our miRNA-based analysis might be effective for identifying prognostic and therapeutic molecules in LUAD.
Insight of Weathering Processes Based on Monitoring Surface Characteristic of Tropical Wood Species
Authors: Jankowska Agnieszka , Rybak Katarzyna , Nowacka Malgorzata , Boruszewski Piotr
Published: September 2020

The main aim of the presented research was to compare the influence of selected ageing factors, such as UV radiation and complex artificial weathering methods, on the colour, wettability and roughness changes in garapa, tatajuba, courbaril and massaranduba from South America—tropical wood species that are popular for external usage in European countries. Both processes caused wood surfaces to become darker and turn to shades of brown. The highest total colour changes were shown in courbaril wood (wood with the highest extractives content). The wood surface roughness demonstrated variation, depending on the wood section and measurement direction, and increased after ageing treatments. Changes in surface contact angle were significant after the inclusion of water and drying in the weathering process (wettability decreased). Anatomical analyses of the tested tropical woods revealed structural changes after used artificial weathering treatments (distortion between cell elements, degradation of the middle lamella, micro-cracks in cell walls, thinning and degradation of parenchyma cells, cracks along pits within vessels). As a result of desorption tension, the changes caused by UV irradiation were much smaller than those caused by full artificial weathering. Fourier-transform infrared spectroscopy (FTIR) analysis indicated the occurrence of lignin and hemicelluloses oxidative changes after the weathering process which resulted in the formation of carbonyl and carboxyl compounds. The depolymerisation of cellulose was also identified. The results show that the observed changes may affect the long-term durability of finishes applied over wood subjected to weathering factors for a short period before finishing.
Characterization and Luminescence of Eu3+- and Gd3+-Doped Hydroxyapatite Ca10(PO4)6(OH)2
Authors: Paterlini Veronica , Bettinelli Marco , Rizzi Rosanna , El Khouri Asmaa , Rossi Manuela , Della Ventura Giancarlo , Capitelli Francesco
Published: September 2020

Luminescence properties of europium-doped Ca10-xEux(PO4)6(OH)2 (xEu = 0, 0.01, 0.02, 0.10 and 0.20) and gadolinium-doped hydroxyapatite Ca9.80Gd0.20(PO4)6(OH)2 (HA), synthesized via solid-state reaction at T = 1300 °C, were investigated using scanning electron microscopy (SEM), powder X-ray diffraction (PXRD), Fourier transform infrared (FTIR), and luminescence spectroscopy. Crystal structure characterization (from unit cell parameters determination to refined atomic positions) was achieved in the P63/m space group. FTIR analyses show only slight band shifts of (PO4) modes as a function of the rare earth concentration. Structural refinement, achieved via the Rietveld method, and luminescence spectroscopy highlighted the presence of dopant at the Ca2 site. Strong luminescence was observed for all Eu- and Gd-doped samples. Our multi-methodological study confirms that rare-earth (RE)-doped synthetic hydroxyapatites are promising materials for bio-imaging applications.
Long-Term Effects of Kasai Portoenterostomy for Biliary Atresia Treatment in Russia
Authors: Degtyareva Anna , Razumovskiy Alexander , Kulikova Nadezhda , Ratnikov Sergey , Filippova Elena , Gordeeva Ekaterina , Albegova Marina , Rebrikov Denis , Puchkova Anna
Published: September 2020

This prospective study enrolled 144 patients after surgical treatment of biliary atresia in early infancy. We analyzed the immediate effectiveness of the surgery and the age-related structure of complications in the up to 16-year follow-up. The immediate 2-year survival rate after the surgery constituted 49.5%. At the time of this writing, 17 of the patients had celebrated their 10th birthdays with good quality of life and no indications for transplantation of the liver. The obtained results underscore the critical importance of surgical correction of biliary atresia by Kasai surgery in the first 60 days of life and subsequent dynamic follow-up of patients for the purpose of the early detection and timely correction of possible complications.
The Conundrum of Poor Ovarian Response: From Diagnosis to Treatment
Authors: Giannelou Polina , Simopoulou Mara , Grigoriadis Sokratis , Makrakis Evangelos , Kontogeorgi Adamantia , Pantou Agni , Galatis Dionysios , Kalampokas Theodoros , Bakas Panagiotis , Bolaris Stamatis , Pantos Konstantinos , Sfakianoudis Konstantinos
Published: September 2020

Despite recent striking advances in assisted reproductive technology (ART), poor ovarian response (POR) diagnosis and treatment is still considered challenging. Poor responders constitute a heterogeneous cohort with the common denominator of under-responding to controlled ovarian stimulation. Inevitably, respective success rates are significantly compromised. As POR pathophysiology entails the elusive factor of compromised ovarian function, both diagnosis and management fuel an ongoing heated debate depicted in the literature. From the criteria employed for diagnosis to the plethora of strategies and adjuvant therapies proposed, the conundrum of POR still puzzles the practitioner. What is more, novel treatment approaches from stem cell therapy and platelet-rich plasma intra-ovarian infusion to mitochondrial replacement therapy have emerged, albeit not claiming clinical routine status yet. The complex and time sensitive nature of this subgroup of infertile patients indicates the demand for a consensus on a horizontally accepted definition, diagnosis and subsequent effective treating strategy. This critical review analyzes the standing criteria employed in order to diagnose and aptly categorize POR patients, while it proceeds to critically evaluate current and novel strategies regarding their management. Discrepancies in diagnosis and respective implications are discussed, while the existing diversity in management options highlights the need for individualized management.
A Novel Isothermal Compression Method for Energy Conservation in Fluid Power Systems
Authors: Ren Teng , Xu Weiqing , Jia Guan-Wei , Cai Maolin
Published: September 2020

Reducing carbon emissions is an urgent problem around the world while facing the energy and environmental crises. Whatever progress has been made in renewable energy research, efforts made to energy-saving technology is always necessary. The energy consumption from fluid power systems of industrial processes is considerable, especially for pneumatic systems. A novel isothermal compression method was proposed to lower the energy consumption of compressors. A porous medium was introduced to compose an isothermal piston. The porous medium was located beneath a conventional piston, and gradually immerged into the liquid during compression. The compression heat was absorbed by the porous medium, and finally conducted with the liquid at the chamber bottom. The heat transfer can be significantly enhanced due to the large surface area of the porous medium. As the liquid has a large heat capacity, the liquid temperature can maintain constant through circulation outside. This create near-isothermal compression, which minimizes energy loss in the form of heat, which cannot be recovered. There will be mass loss of the air due to dissolution and leakage. Therefore, the dissolution and leakage amount of gas are compensated for in this method. Gas is dissolved into liquid with the pressure increasing, which leads to mass loss of the gas. With a pressure ratio of 4:1 and a rotational speed of 100 rpm, the isothermal piston decreased the energy consumption by 45% over the conventional reciprocation piston. This gain was accomplished by increasing the heat transfer during the gas compression by increasing the surface area to volume ratio in the compression chamber. Frictional forces between the porous medium and liquid was presented. Work to overcome the frictional forces is negligible (0.21% of the total compression work) under the current operating condition.
Implementing Government Elementary Math Exercises Online: Positive Effects Found in RCT under Social Turmoil in Chile
Authors: Araya Roberto , Diaz Karina
Published: September 2020

The impact of online math programs depends on its implementation, especially in vulnerable populations from developing countries. An existing online platform was adapted, at the request of the Chilean Ministry of Education, to exclusively include exercises previously designed and tested by a paper-based government program for elementary school. We carried out a cluster-randomized controlled trial (RCT) with 50 fourth grade classrooms. Treatment classrooms used the platform in a weekly 90-min math session. Due to a social instability outbreak in the country, a large unexpected disruption with huge absenteeism occurred in the second half of the semester, which turned this study into a unique opportunity to explore the robustness of the platform’s effects on students’ learning. Using multiple imputation and multilevel models, we found a statistically significant effect size of 0.13, which corresponds to two extra months of learning. This effect is meaningful for four reasons. First, it has double the effect of the paper-based version. Second, it was achieved during one semester only. Third, is half that obtained with the platform for a complete year with its own set of exercises and with two sessions per week instead of one. Fourth, it was attained in a semester with a lot of absenteeism.
A Model to Improve Granular Temperature in CFD-DEM Simulations
Authors: Yu Yaxiong , Zhao Li , Li Yu , Zhou Qiang
Published: September 2020

CFD-DEM (computational fluid dynamic-discrete element method) is a promising approach for simulating fluid–solid flows in fluidized beds. This approach generally under-predicts the granular temperature due to the use of drag models for the average drag force. This work develops a simple model to improve the granular temperature through increasing the drag force fluctuations on the particles. The increased drag force fluctuations are designed to match those obtained from PR-DNSs (particle-resolved direct numerical simulations). The impacts of the present model on the granular temperatures are demonstrated by posteriori tests. The posteriori tests of tri-periodic gas–solid flows show that simulations with the present model can obtain transient as well as steady-state granular temperature correctly. Moreover, the posteriori tests of fluidized beds indicated that the present model could significantly improve the granular temperature for the homogenous or slightly inhomogeneous systems, while it showed negligible improvement on the granular temperature for the significantly inhomogeneous systems.
Ceiling and Wall Illumination, Utilance, and Power in Interior Lighting
Authors: Pracki Piotr , Dziedzicki Michał , Komorzycka Paulina
Published: September 2020

The common use of electric lighting in interiors has led to the need to search for user- and environmentally-friendly solutions. In this research, the impact of the luminaires and room parameters on the selected parameters of general lighting in interiors was assessed. To achieve the objective of this work, a computer simulation and statistical analysis of results were conducted. The illuminance uniformity on work plane, ceiling and wall relative illuminances, utilance, and normalized power density of lighting installations for 432 situations were analyzed in detail. The scenarios were varied in terms of room size, reflectance, lighting class, luminaire downward luminous intensity distribution, and layout. The lighting class was a factor having the highest impact on ceiling and wall illumination, utilance, and power. It was also shown that the impact of lighting class on ceiling illumination, utilance and power, was different in interiors of various sizes. The impact of reflectances and luminaire layouts on the analyzed parameters was significantly lower. The results also demonstrated that the use of different lighting classes gave the possibility of reducing the power of general lighting in interiors at a level of 30% on average. Based on the results, a classification of energy efficiency in general lighting in interiors was also proposed. Understanding the correlations between the lighting system used and the effects achieved is helpful in obtaining comfortable and efficient lighting solutions in interiors.
Organic Carbon Stabilization Mechanisms in Mangrove Soils: A Review
Authors: Kida Morimaru , Fujitake Nobuhide
Published: September 2020

Despite the recognized organic carbon (OC) sequestration potential of mangrove forests, the ongoing climate change and anthropogenic disturbances pose a great threat to these ecosystems. However, we currently lack the ability to mechanically understand and predict the consequences of such impacts, primarily because mechanisms underlying OC stabilization in these ecosystems remain elusive. Research into OC stabilization has focused on terrestrial soils and marine sediments for decades, overlooking the vegetated coastal ecosystems including mangroves. In terrestrial soils and marine sediments, it is widely accepted that OC stabilization is the integrated consequence of OM’s inherent recalcitrance, physical protection, and interactions with minerals and metals. However, related discussion is rarely done in mangrove soils, and recalcitrance of roots and high net ecosystem production (high primary production and low heterotrophic respiration) have been considered as a primary OC sequestration mechanism in mangrove peat and mineral soils, respectively. This review presents the available information on the mechanisms underlying OC stabilization in mangrove soils and highlights research questions that warrant further investigation. Primary OC stabilization mechanisms differ between mangrove peat and mineral soils. In mangrove mineral soils, physico-chemical stabilization processes are important, yet grossly understudied OC stabilization mechanisms. In mangrove peat, recalcitrance of mangrove roots and the inhibition of phenoloxidase under the anoxic condition may be the primary OC stabilization mechanisms. Salinity-induced OC immobilization likely plays a role in both type of soils. Finally, this review argues that belowground production and allochthonous inputs in mangrove forests are likely underestimated. More studies are needed to constrain C budgets to explain the enigma that mangrove OC keeps accumulating despite much higher decomposition (especially by large lateral exports) than previously considered.
Direct Numerical Simulation of Water Droplets in Turbulent Flow
Authors: Ren Weibo , Reutzsch Jonathan , Weigand Bernhard
Published: September 2020

Details on the fall speeds of raindrops are essential in both applications and natural events, such as rain-rate retrieval and soil erosion. Here, we examine the influence of turbulence on the terminal velocity of two water drops of different sizes. For the first time, computations of droplets in turbulent surroundings are conducted with a direct numerical simulation code based on a volume of fluid method. Both the drop surface deformation and internal circulation are captured. The turbulence intensity at the inflow area, as well as the turbulence length scale are varied. In turbulent flow, we find a decline in the terminal velocities for both drops. Based on the study of the wake flow characteristics and drop surface deformation, the decrease in the terminal velocity is found to be directly linked to a shortening of the wake recirculation region resulting from an earlier and more drastic increase in the turbulence kinetic energy in the shear layer. The turbulent surroundings trigger substantial rises in the drop axis ratio amplitude and a slight increase in the drop oscillation frequency, but barely influence the time-averaged drop axis length.
Comparison of Traditional and Novel Drying Techniques and Its Effect on Quality of Fruits, Vegetables and Aromatic Herbs
Authors: Calín-Sánchez Ángel , Lipan Leontina , Cano-Lamadrid Marina , Kharaghani Abdolreza , Masztalerz Klaudia , Carbonell-Barrachina Ángel A. , Figiel Adam
Published: September 2020

Drying is known as the best method to preserve fruits, vegetables, and herbs, decreasing not only the raw material volume but also its weight. This results in cheaper transportation and increments the product shelf life, limiting the food waste. Drying involves the application of energy in order to vaporize and mobilize the moisture content within the porous products. During this process, the heat and mass transfer occurs simultaneously. The quality of dehydrated fruits, vegetables, and aromatic herbs is a key problem closely related to the development and optimization of novel drying techniques. This review reports the weaknesses of common drying methods applied for fruits, vegetables, and aromatic herbs and the possible options to improve the quality of dried products using different drying techniques or their combination. The quality parameters under study include color, bulk density, porosity, shrinkage, phytochemicals, antioxidant capacity, sugars, proteins, volatile compounds, and sensory attributes. In general, drying leads to reduction in all studied parameters. However, the behavior of each plant material is different. On the whole, the optimal drying technique is different for each of the materials studied and specific conditions must be recommended after a proper evaluation of the drying protocols. However, a novel or combined technique must assure a high quality of dried products. Furthermore, the term quality must englobe the energy efficiency and the environmental impact leading to production of sustainable dried products.
Podravka and Slavonka Varieties of Pepper Seeds (Capsicum annuum L.) as a New Source of Highly Nutritional Edible Oil
Authors: Cvetković Tanja , Ranilović Jasmina , Gajari Davorka , Tomić-Obrdalj Helena , Šubarić Drago , Moslavac Tihomir , Cikoš Ana-Marija , Jokić Stela
Published: September 2020

The aim of this study was to evaluate Croatian pepper seed varieties (Podravka and Slavonka) as a new source of added value ingredients. Pepper seed is mostly considered a by-product. For the first time, the pepper (Capsicum annuum L.) seeds of the Slavonka and Podravka varieties were examined as the source material for oil production by the two methods of extraction: cold pressing (CP) and supercritical CO2 extraction (SC-CO2). Further, fatty acid profile, tocopherols, and sensory analysis of the oils were examined, as well as the chemical characteristics and antioxidant potential of seed flour. The antioxidant potential of pepper seed flour was different between varieties (Podravka 107 antioxidant unit (AU); Slavonka 70 antioxidant unit (AU)). The Podravka variety pepper seed oil has shown higher γ-tocopherol content (CP 80.1 mg/100 g; SC-CO2 extraction 65.3 mg/100 g) than the Slavonka variety (CP 65.3 mg/100 g; SC-CO2 extraction 16.0 mg/100 g). According to the obtained results, cold pressing (CP) would be a more favourable method for pepper seed oil extraction, taking into account sensory evaluation and nutritional quality. The pepper seed oil has potential for culinary application with a nutritional quality comparable to vegetable oils of a higher price class.
Demanding New Honey Qualitative Standard Based on Antibacterial Activity
Authors: Bucekova Marcela , Bugarova Veronika , Godocikova Jana , Majtan Juraj
Published: September 2020

Honey is a functional food with health-beneficial properties and it is already used as a medical device in wound care management. Whether ingested orally or applied topically, honey must fulfill the requirements of international standards based on physicochemical characteristics. However, there is an urgent need for some additional standards reflecting biological properties. The aim of the study was to evaluate the antibacterial activity of 36 commercial honey samples purchased from supermarkets and local food shops and compare their efficacy to that of three honey samples from local beekeepers and three types of medical-grade honey. Furthermore, the hydrogen peroxide (H2O2) content and protein profile were assessed in all honey samples. Analysis of the antibacterial activity of commercial honeys revealed that 44% of tested samples exhibited low antibacterial activity, identical to the activity of artificial honey (sugars only). There was a significant correlation between the overall antibacterial activity and H2O2 content of honey samples. However, in some cases, honey samples exhibited high antibacterial activity while generating low levels of H2O2 and vice versa. Honey samples from local beekeepers showed superior antibacterial activity compared to medical-grade honeys. The antibacterial activity of honey can be easily altered by adulteration, thermal treatment or prolonged storage, and therefore it fulfils strict criteria to be suitable new additional quality standard.