bluuebunny/crossref_metadata_2025 huggingface dataset:
Water deficit and potassium affect carbon isotope composition in cassava bulk leaf material and extracted carbohydrates
Authors: Van Laere Jonas , Merckx Roel , Hood-Nowotny Rebecca , Dercon Gerd
Published: October 2023

Cassava (Manihot esculenta Crantz) is an important root crop, which despite its drought tolerance suffers considerable yield losses under water deficit. One strategy to increase crop yields under water deficit is improving the crop’s transpiration efficiency, which could be achieved by variety selection and potassium application. We assessed carbon isotope composition in bulk leaf material and extracted carbohydrates (soluble sugar, starch, and cellulose) of selected leaves one month after inducing water deficit to estimate transpiration efficiency and storage root biomass under varying conditions in a greenhouse experiment. A local and improved variety were grown in sand, supplied with nutrient solution with two potassium levels (1.44 vs. 0.04 mM K+) and were subjected to water deficit five months after planting. Potassium application and selection of the improved variety both increased transpiration efficiency of the roots with 58% and 85% respectively. Only in the improved variety were 13C ratios affected by potassium application (up to - 1.8‰ in δ13C of soluble sugar) and water deficit (up to + 0.6‰ in δ13C of starch and soluble sugar). These data revealed a shift in substrate away from transitory starch for cellulose synthesis in young leaves of the improved variety under potassium deficit. Bulk δ13C of leaves that had fully developed prior to water deficit were the best proxies for storage root biomass (r = - 0.62, r = - 0.70) and transpiration efficiency (r = - 0.68, r = - 0.58) for the local and improved variety respectively, making laborious extractions redundant. Results obtained from the youngest fully developed leaf, commonly used as a diagnostic leaf, were complicated by remobilized assimilates in the improved variety, making them less suitable for carbon isotope analysis. This study highlights the potential of carbon isotope composition to assess transpiration efficiency and yield, depending on the chosen sampling strategy as well as to unravel carbon allocation processes.
Changes in bud bank and their correlation with plant community composition in degraded alpine meadows
Authors: Li Yuan , Bao Gensheng , Zhang Peng , Feng Xiaoyun , Ma Jingjuan , Lu Hainian , Shi Hongxiao , Wei Xiaoxing , Tang Bingming , Liu Kai
Published: October 2023

Bud banks are considered a crucial factor in regulating the species composition of grassland communities and maintaining the ecological function of alpine grasslands. However, few studies have paid attention to the dynamic changes of bud banks from undisturbed to severely degraded alpine meadows. Therefore, this study examined the correlations between plant diversity and bud bank traits at different stages of alpine meadows degradation. Grass biomasses and plant diversity were found to be highest in moderately degraded meadows, and sedge biomasses were highest in lightly degraded meadows. Lack of disturbance and moderate disturbance by herbivores increased the bud bank density of alpine meadows. Consistent with the changes in bud bank density, bud bank diversity was highest in undisturbed meadows. The structural equation model indicated that the densities of rhizome and the densities and diversities of tiller buds play crucial roles in facilitating the greater diversity of the plant community. Our findings suggest that the diversities and densities of rhizome and tiller buds in the degradation stages are synchronized with changes in plant diversity, and in the regenerative ability of bud banks, which largely determine the outcome of restoration in degraded meadows. These findings could provide a frame of reference for effectively restoring degraded alpine regions by regenerating bud banks. The potential driving force and renewal capacity of bud banks should be taken into account in restoring the Qinghai-Tibet Plateau’s degraded meadow.
Daily sound exposure of hearing aids users during COVID-19 pandemic in Europe
Authors: Sun Kang , Szatmari Tiberiu-Ioan , Pasta Alessandro , Bramsløw Lars , Wendt Dorothea , Christensen Jeppe H. , Pontoppidan Niels H.
Published: October 2023

Introduction

This study aimed to investigate the daily sound exposure of hearing aid (HA) users during the COVID-19 pandemic, with a specific focus on the impact of different governance intervention levels.

Methods

Modern HA technology was employed to measure and compare the sound exposure of HA users in three distinct periods: pre-pandemic, and two 14-day periods during the pandemic, corresponding to varying levels of governance interventions. The study sample comprised a total of 386 HA users in Europe during the pandemic, with daily sound exposure data collected as part of the main dataset.

Results

The results revealed that, during the pandemic, the equivalent continuous sound pressure level (SPL) experienced by HA users decreased, while the signal-to-noise ratio (SNR) increased compared to the pre-pandemic period. Notably, this impact was found to be more pronounced (p < 0.05) when individuals were subjected to stronger governance intervention levels, characterized by lower SPL and higher SNR.

Discussion

This study highlights the changes in daily sound exposure experienced by HA users during the COVID-19 pandemic, particularly influenced by the extent of governance interventions that restricted social activities. These findings emphasize the importance of considering the effects of pandemic-related governance measures on the sound environments of HA users and have implications for audiological interventions and support strategies during similar crises.
Functional limitation and happiness among older adults: the multiple mediating role of intergenerational support and intergenerational relationship
Authors: Zhou Angdi , Song Yiwen , Li Xinru , Hu Bingqin , Chen Yitong , Cui Peiyao , Li Jinghua
Published: October 2023

Objectives

This study aimed to investigate the relationship between the functional limitation and happiness among Chinese older people and examined the multiple mediating effects of intergenerational support (instrumental support and financial support) and intergenerational relationship.

Method

Data was drawn from the Chinese Family Panel Survey (CFPS) 2018 and 2020. Structural equation modeling was adopted to analyze the association among functional limitations, intergenerational support, intergenerational relationship, and the older adults happiness.

Results

There was a significant association between the functional limitations and the lower happiness levels among the older adults. The instrumental support from adult children positively mediated the relationship between the functional limitation and the happiness. However, intergenerational relationships were reduced due to the dysfunction of the older adults, and played a negatively mediated role between the functional limitation and the happiness. In addition, instrumental and financial support play chain-mediating roles between functional limitation and happiness in older adults through intergenerational relationships.

Conclusion

Intergenerational relationships and instrumental support enhance the happiness of older adults with functional impairments, but their role is limited by the changing structure of modern families. Long-term care programs combined with the intergenerational support from families for people with functional impairments in old age would be more effective to reduce the burden on adult children and maintain the quality of life of the older adults.
Metabolic diseases and healthy aging: identifying environmental and behavioral risk factors and promoting public health
Authors: Zhang Kexin , Ma Yujie , Luo Youhong , Song Yixin , Xiong Guoji , Ma Yanhui , Sun Xiaodong , Kan Chengxia
Published: October 2023

Aging is a progressive and irreversible pathophysiological process that manifests as the decline in tissue and cellular functions, along with a significant increase in the risk of various aging-related diseases, including metabolic diseases. While advances in modern medicine have significantly promoted human health and extended human lifespan, metabolic diseases such as obesity and type 2 diabetes among the older adults pose a major challenge to global public health as societies age. Therefore, understanding the complex interaction between risk factors and metabolic diseases is crucial for promoting well-being and healthy aging. This review article explores the environmental and behavioral risk factors associated with metabolic diseases and their impact on healthy aging. The environment, including an obesogenic environment and exposure to environmental toxins, is strongly correlated with the rising prevalence of obesity and its comorbidities. Behavioral factors, such as diet, physical activity, smoking, alcohol consumption, and sleep patterns, significantly influence the risk of metabolic diseases throughout aging. Public health interventions targeting modifiable risk factors can effectively promote healthier lifestyles and prevent metabolic diseases. Collaboration between government agencies, healthcare providers and community organizations is essential for implementing these interventions and creating supportive environments that foster healthy aging.
Effect of different disinfectants on preventing asymptomatic bacteriuria and catheter-related urinary tract infection: a network meta-analysis
Authors: Sun Zhi Hong , Ma Xue Wei , Sun Wei , Wei Ying Ji , Li Yi Zhen , Wang Dan , Zhou Chang Xi , Xu Guo Gang , Zhang Gui Zhi
Published: October 2023

Objective

To analyze the effect of different types of disinfectants for perineum and urethral meatus cleaning in preventing catheter-associated asymptomatic bacteriuria and catheter-associated urinary tract infection (CAUTI).

Methods

Chinese and English databases were searched to collect randomized controlled trials (RCTs) of different disinfectants for perineum and urethral meatus cleaning to prevent CAUTI, and the positive rates of urine culture with different cleaning methods were contrasted by network meta-analysis.

Results

A total of 18 RCTs were included in this study to analyze the effect of 10 disinfectants in preventing CAUTI. The disinfectants were 0.1% chlorhexidine (CHG), 0.5% CHG, 2% CHG, 0.1% benzalkonium bromide (DBDAB), 0.05% iodophor, 0.5% iodophor, acidic oxidizing potential water (EOW), normal saline (NS), Shehuang lotion, and sterile water. Meta-analysis showed that the positive rates of urine culture in the 0.5% CHG cluster, EOW cluster, 0.5% iodophor cluster, and Shehuang lotion cluster were remarkably lower than that in the 0.1% DBDAB cluster (P < 0.05). The positive rate of urine culture in the 0.5% CHG cluster was remarkably lower than that in the 0.05% iodophor cluster (P < 0.05), whereas the positive rates of urine culture in the 0.5% CHG cluster, 0.5% iodophor cluster, Shehuang lotion cluster, and EOW were remarkably lower than that in the normal saline cluster (P < 0.05). The positive rate of urine culture in the 0.5% CHG cluster and EOW cluster were remarkably lower than those in the sterile water cluster (P < 0.05). The results of the area under the cumulative ranking probability plot (SUCRA) analysis showed that the probability ranking of the preventive effect of different disinfectants was as follows: 0.5% CHG > Shehuang lotion > EOW > 0.5% Iodophor > 2% CHG > 0.1% CHG > 0.05% Iodophor > Sterile Water > 0.1% DBDAB > Saline.

Conclusion

0.5% CHG, Shehuang lotion, EOW, and 0.5% iodophor can be used to clean the perineum and urethral meatus in patients with indwelling catheters.

Systematic review registration

Chinese Clinical Trial Registry (ChiCTR), identifier ChiCTR2100052260.
Can cardiac pressure-volume catheters improve urodynamic assessment? an ex-vivo proof-of-concept
Authors: Jahren Silje Ekroll , Obrist Dominik , Haenggi Matthias , Burkhard Fiona , Clavica Francesco
Published: October 2023

Aims

To explore the feasibility of using pressure-volume (PV) catheters for cystometry. These catheters are well-established in cardiovascular research for simultaneous pressure and volume measurements in the left ventricle.

Methods

Urinary bladders with urethras were collected from domestic pigs for ex-vivo testing. Using a pump, bladders were filled up to 500ml at five different filling rates (15, 20, 25, 30, 35ml/min), and the resulting pressures and volumes were measured using a PV catheter. The bladder were compressed externally in three different areas (central, apex and outlet) to assess the PV catheter’s ability to detect local changes in bladder volume.

Results

Bladder pressure remained below 10cmH2O for all bladder filling rates. Volume measurements were compared with the volumes instilled by the pump (ground truth), proving high reproducibility and accuracy of the PV catheter measurements up to 400ml. Using the different sensing units of the PV catheter, local changes in bladder volumes could be identified and quantified.

Conclusion

The main advantage of PV catheters, compared to existing technology used in urology (e.g. conventional urodynamic testing), is the possibility to i) simultaneously measure bladder pressure and volumes and ii) identify local changes in bladder volume (e.g. caused by non-voiding contractions). Both could be useful in the clinical setting to improve the diagnosis and treatment of the Lower Urinary Tract Dysfunction (e.g. overactive/underactive bladder).
Critical food systems education in university student-run food initiatives: learning opportunities for food systems transformation
Authors: Deskin Zoë Y. , Harvey Blane
Published: October 2023

Introduction

Student-run Campus Food Systems Alternatives (CFSA) have been proposed as spaces which have the potential to advance Critical Food Systems Education (CFSE) – the objective of which is to motivate students to act toward radical food systems transformation on community and systemic levels. Evidence on how learning dynamics in CFSA drive student participants to develop critical perspectives on food systems is limited, however. This paper seeks to address this gap by exploring how critical and transformative learning happens in these informal and student-run spaces, by detailing a multi-case study of students’ learning experiences in four student-run CFSA on the McGill University campus.

Methods

Data on students’ learning experiences was collected through observational field notes of CFSA activities and semi-structured Interviews with student facilitators. Thematic and cross-case analysis was performed with interview data.

Results

Analysis of students’ described learning experiences in CFSA revealed three broad categories of learning dynamics which drive students’ learning about food systems and their willingness to act for food systems change: hands-on work in informal spaces, social connection and engagement between student participants, and engagement with the beyond-campus community.

Discussion

Engagement with the beyond-campus community via CFSA, particularly that which involved exposure to food-related injustice in marginalized communities, was found to be particularly important in driving student participants’ critical reflection on food systems and willingness to act toward food justice. A lack of intentional critical reflective practice was however observed in CFSA, calling into question how this practice can be driven in campus food initiatives without compromising their student-run and informal structures.
The impact of food safety scandals on pork prices from consumer concern perspective: evidence from China
Authors: Wan Wenyu , Tao Jianping
Published: October 2023

In the era of fast communication, consumer attention is a hidden demand signal that cannot be ignored. This study investigates the impacts of media coverages on pork prices from the perspective of consumer concerns. We employ monthly panel data spanning from January 2018 to June 2021 at the provincial level. Specifically, the study investigates the mediating role of consumer attention, which is categorized into “active attention” and “passive attention,” in the relationship between food scandal report and pork prices. The empirical results suggest a negative short-term correlation between food scandals coverages and pork prices, and reveal that passive-guided consumer attention plays a more significant mediating role than active attention in this relationship. Furthermore, a heterogeneity analysis based on the North–South partition indicates that the mediating transmission effect of consumer attention is more pronounced in regions with relatively stable supply. Overall, this study contributes to the existing theoretical literature on the impact of media coverages on agricultural prices, and provides practical implications for the government to regulate media coverages, and guide consumers to respond appropriately to food safety risks.
The outcomes of proximal femur replacement with hemiarthroplasty after tumor resection in individuals with Parkinson's disease
Authors: Zhao Zhiqing , Wang Jichuan , Guo Yu , Niu Jianfang , Guo Wei , Yang Rongli , Yan Taiqiang , Tang Xiaodong
Published: October 2023

Background

Patients with neurological disorders often experience a high incidence of postoperative complications following proximal femur replacement (PFR) surgery. The orthopaedist faces a significant difficulty in treating Parkinson's disease (PD) because of the weakened bone condition, excessive muscle tension, and increased risk of fractures. The objective of this research is to assess the impact of PD on PFR following tumor removal.

Methods

A retrospective study was conducted from 2010 to 2020, focusing on a solitary institution, analyzing 9 patients diagnosed with PD who underwent PFR with hemiarthroplasty as a result of tumor removal. The study consists of 2 men and 7 women, with an average age of 71 (SD, 12) years. We assessed the outcomes after surgery in terms of pain management, quality of life, functional ability, occurrence of complications, and survival durations.

Results

All nine patients underwent planned surgeries. Intraoperative complications was not observed. The average length of the follow-up period was 24 (SD, 20) months, ranging from 8 to 72 months. Despite the fact that 8 patients passed away due to tumor progression, the endoprostheses were still well at that point. The preoperative VAS score of 7 (SD, 1.87) decreased to a postoperative score of 2 (SD, 1.32). The KPS was improved to73 (SD, 7) from 52 (SD, 14), postoperatively. Post-surgery, there were notable enhancements in both pain levels and the overall quality of life scores. Following the surgical procedure, individuals are able to ambulate steadily, resuming their regular daily routines. Living patients had an average MSTS score of 21 (SD, 2.5), ranging from 17 to 25. In total, there were four (44.4%) patients suffered complications after surgery, comprising of one wound dehiscence, one prosthetic fracture, one hip dislocation, and one local recurrence.

Conclusions

Significant improvements in function and pain relief can be achieved through PFR with hemiarthroplasty following tumor removal in patients with PD. The implementation of thorough preparation and carefull nursing results in reduced complications and improved outcomes in PD patients.
Methodological comparison of cancellation versus two-way choice spatial attention tests in humans and dogs
Authors: Kis Anna , Radics Eszter , Bolló Henrietta , Topál József
Published: October 2023

Introduction

Behavioural problems in family dogs are amongst the leading reasons for relinquishment to shelters which adversely affects animal welfare. Recent research suggests that certain problematic behavioural patterns might be analogous to human psychiatric disorders. Veterinary diagnosis of such conditions, however, is scarce, probably due to the lack of appropriate measurement tools. The current study focuses on dog behaviour resembling the human hemispatial neglect condition, which manifests itself as a deficit in attention to and awareness of one side of the space.

Methods

Healthy human subjects (N = 21) and adult family dogs (N = 23) were tested with tools aimed to measure spatial attention. Tests administered to humans included validated paper and pencil neuropsychological tools to assess hemispatial neglect (cancellation tasks), as well as the canine version of that task (visuo-spatial search task). Dogs were tested with the same visuo-spatial search task as well as a two-way choice task.

Results

Results show that both in case of dogs and humans the visuo-spatial search task detects individual variation in subjects’ side preferences. However, subjects’ performance in the different tasks were not related.
Dietary supplementation with olive oil co-products rich in polyphenols: a novel nutraceutical approach in monogastric animal nutrition
Authors: Ferlisi Flavia , Tang Jiayong , Cappelli Katia , Trabalza-Marinucci Massimo
Published: October 2023

In recent years, the increased demand for agri-food products to feed livestock species has stimulated research to identify novel solutions for the valorization of natural waste, according to the modern concept of a circular economy. Numerous studies have shown the use of plant-derived and agro-industrial co-products that are sources of bioactive molecules for preparing animal feeds. Supplementation with co-products derived from the extraction of olive oil (i.e., olive pomace, olive mill wastewater, olive cake and olive leaf) in diet has been widely considered in recent decades, because these wastes are produced in high quantity and their re-use represents an innovative economic and environmental strategy. Olive oil co-products are characterized by various bioactive molecules such as polyphenols, carbohydrates, proteins, and lipids. Among them, polyphenols are the nutraceuticals most studied, showing to promote health effects in both humans and animals. Olive oil co-products and their phenolic extracts have shown many beneficial and promising effects when added to the diets of monogastric animals, by improving performance parameters and maintaining the oxidative status of meat and derived products. This review provides an update on the use of olive co-products in monogastric animal (swine, poultry and rabbit) diets and their effects on the productive performance, meat quality characteristics and gut health status.
Confirmation of canine acanthomatous ameloblastoma using RAS Q61R immunohistochemical staining of formalin-fixed paraffin-embedded tissues
Authors: Peralta Santiago , Marcinczyk Magdalena M. , Katt William P. , Duhamel Gerald E.
Published: October 2023

Differentiating canine acanthomatous ameloblastoma (CAA) from oral squamous cell carcinoma (OSCC) based on routine histopathology can be challenging. We have previously shown that more than 95% of CAAs harbor anHRASp.Q61R somatic mutation, while OSCCs carry either wild-type alleles or other MAPK pathway activating mutations (e.g.,HRASp.Q61L,BRAFp.V595E). Given thatHRASp.Q61R mutations are highly prevalent in CAA, we hypothesized that a RAS Q61R-specific rabbit monoclonal antibody may be a useful tool for confirmation of CAA by immunohistochemical (IHC) staining. In the present study, we assessed IHC staining of archived formalin-fixed and paraffin-embedded biopsy samples with a diagnosis of CAA (n = 23), using a RAS Q61R-specific rabbit monoclonal antibody (SP174) and an automated IHC stainer. Negative control samples consisted ofHRASp.Q61R mutation-negative OSCC tumors with either a knownHRASp.Q61L mutation (n = 1),BRAFp.V595E mutation (n = 4), or wild-type corresponding alleles (n = 3). We found that all 23 CAAs showed diffuse and strong membranous RAS Q61R immunoreactivity (100% sensitivity), while none of the 8 OSCCs showed immunoreactivity (100% specificity). The data supports the use of RAS Q61R-specific rabbit monoclonal antibody for diagnostic IHC confirmation of CAA and ruling out OSCC in dogs.
Problem-Driven Scenario Generation for Stochastic Programming Problems: A Survey
Authors: Chou Xiaochen , Messina Enza
Published: October 2023

Stochastic Programming is a powerful framework that addresses decision-making under uncertainties, which is a frequent occurrence in real-world problems. To effectively solve Stochastic Programming problems, scenario generation is one of the common practices that organizes realizations of stochastic processes with finite discrete distributions, which enables the use of mathematical programming models of the original problem. The quality of solutions is significantly influenced by the scenarios employed, necessitating a delicate balance between incorporating informative scenarios and preventing overfitting. Distributions-based scenario generation methodologies have been extensively studied over time, while a relatively recent concept of problem-driven scenario generation has emerged, aiming to incorporate the underlying problem’s structure during the scenario generation process. This survey explores recent literature on problem-driven scenario generation algorithms and methodologies. The investigation aims to identify circumstances under which this approach is effective and efficient. The work provides a comprehensive categorization of existing literature, supplemented by illustrative examples. Additionally, the survey examines potential applications and discusses avenues for its integration with machine learning technologies. By shedding light on the effectiveness of problem-driven scenario generation and its potential for synergistic integration with machine learning, this survey contributes to enhanced decision-making strategies in the context of uncertainties.
XOR Chain and Perfect Secrecy at the Dawn of the Quantum Era
Authors: Lizama-Pérez Luis Adrián
Published: October 2023

In this article, we present a new method that achieves Shannon’s perfect secrecy. To achieve this property, we will introduce the triple XOR cancellation rule. The approach has two execution modes: digital signature and data encryption. We provide perfect secrecy proof of the encryption method. Furthermore, based on our fundamental algorithm, we developed a new strategy for the blockchain system that does not require proof of work (PoW). However, it is a practical mechanism for connecting blocks to the chain. Due to the risk that quantum computers present for current cryptosystems based on prime factorization or discrete logarithm, we postulate that our method represents a promising alternative in the quantum era. We expect our work to have profound implications for the security of communications between mobile devices, the Internet of Things (IoT), and the blockchain.
A Hybrid Improved Symbiotic Organisms Search and Sine–Cosine Particle Swarm Optimization Method for Drone 3D Path Planning
Authors: Xiong Tao , Li Hao , Ding Kai , Liu Haoting , Li Qing
Published: October 2023

Given the accelerated advancement of drones in an array of application domains, the imperative of effective path planning has emerged as a quintessential research focus. Particularly in intricate three-dimensional (3D) environments, formulating the optimal flight path for drones poses a substantial challenge. Nonetheless, prevalent path-planning algorithms exhibit issues encompassing diminished accuracy and inadequate stability. To solve this problem, a hybrid improved symbiotic organisms search (ISOS) and sine–cosine particle swarm optimization (SCPSO) method for drone 3D path planning named HISOS-SCPSO is proposed. In the proposed method, chaotic logistic mapping is first used to improve the diversity of the initial population. Then, the difference strategy, the novel attenuation functions, and the population regeneration strategy are introduced to improve the performance of the algorithm. Finally, in order to ensure that the planned path is available for drone flight, a novel cost function is designed, and a cubic B-spline curve is employed to effectively refine and smoothen the flight path. To assess performance, the simulation is carried out in the mountainous and urban areas. An extensive body of research attests to the exceptional performance of our proposed HISOS-SCPSO.
A Study on the Behavior Characteristics of Air Supply during Tunnel Fires under Natural Ventilation with Multiple Vertical Shafts
Authors: He Lu , Ming Yuyang , Liao Ke , Zhang Haojun , Jia Chenhao , Zhu Guoqing , Tao Haowen
Published: October 2023

This study investigates the behavior of air supply in tunnels with multiple vertical shafts during fire incidents, focusing on natural ventilation dynamics. Numerical simulation is utilized to analyze the effect of different variables on air supply within vertical shafts. The findings reveal that the position of the smoke front significantly influences the direction and flow rate of gases during fire development. The mass flow rate of air supply during the stable fire development stage is influenced by the geometric size and positioning of vertical shafts, with shafts closer to the fire source exhibiting higher air flow rates. To address this issue, this study introduces a predictive model for estimating air flow rates in vertical shafts. This model exhibits a high level of accuracy when compared to simulations, offering a reliable method for predicting air flow rates based on the geometric characteristics of vertical shafts. Overall, this research contributes to understanding the complexities of air supply in tunnels with multiple vertical shafts, aiding in the improvement of natural ventilation strategies during fire incidents.
Numerical Study of the Influence of the Critical Reynolds Number on the Aerodynamic Characteristics of the Wing Airfoil
Authors: Utkina Anna , Kozelkov Andrey , Zhuchkov Roman , Strelets Dmitry
Published: October 2023

The paper reports the results of a study concerned with the influence of the size of the leading edge laminar bubble on the aerodynamic characteristics of the HGR01 airfoil. The completely turbulent and transient flows are considered. The mechanism of the appearance and interaction of laminar and turbulent flow separation near the leading and trailing edges of the airfoil is studied in detail. In the paper, the dependence of aerodynamic forces on the critical Reynolds number for the HGR01 airfoil is discussed. It has been established that the separation bubble at the leading edge can only be obtained using the laminar–turbulent transition model. Fully turbulent models are not able to show this feature of the airfoil flow. Graphs of the lift coefficient as a function of the critical Reynolds number, as well as the pressure distribution as a function of the size of the laminar bubble, are shown.
An Extended Analysis of Temperature Prediction in Italy: From Sub-Seasonal to Seasonal Timescales
Authors: Giunta Giuseppe , Ceppi Alessandro , Salerno Raffaele
Published: October 2023

Earth system predictions, from sub-seasonal to seasonal timescales, remain a challenging task, and the representation of predictability sources on seasonal timescales is a complex work. Nonetheless, advances in technology and science have been making continuous progress in seasonal forecasting. In a previous paper, a performance for temperature prediction by a modelling system named e-kmf® was carried out in comparison with observations and climatology for a year of data; a low level of predictability in the sub-seasonal range, particularly in the second month, was observed over the Italian peninsula. Therefore, in this study, we focus our investigations specifically on the performance between the fifth and the eighth week of temperature forecasts over six years of simulations (2012–2018) to investigate the capability of the weather model to better reproduce the behavior of temperatures in the second month of the forecast. Although some differences in seasons are present, results have globally shown how temperature predictions have the potential to be quite skillful, with an average skill score of about 68%, with climatology used as reference; additionally, an overall anomaly correlation coefficient equal to 0.51 was shown, providing useful information for applications in planning, sales, and supply of natural energy resources.
Jewish Surname Changes (Sampling of Prague Birth Registries 1867–1918)
Authors: Dvořáková Žaneta
Published: October 2023

The study focuses on changes of surnames among Czech and Moravian Jews. The changes are tracked until the start of the German occupation in 1939. The source material is comprised of Jewish birth registers from 1867 to 1918 from Prague, as this was the most populous Jewish community of the region. These records are part of fund No. 167 stored in the Czech National Archive. More than 17,000 Jewish children were born in Prague during this period and only 350 of them changed their surnames. Surnames were mostly changed by young men under the age of 30. A large wave of renaming occurred mainly at the beginning of the 1920s shortly after the formation of Czechoslovakia (1918). Renaming was part of the assimilation process but was not connected to conversion to Christianity. The main goal was the effort to remove names perceived as ethnically stereotypical, which could stigmatize their bearers (e.g., Kohn, Löwy, Abeles, Taussig, Goldstein, etc.). Characteristic of the new surnames was the effort to preserve the same initial letter from the original surname. The phenomenon is compared with the situation in neighboring countries (Germany, Hungary, and Poland).
The Influence of the Spectral Composition and Light Intensity on the Morphological and Biochemical Parameters of Spinach (Spinacia oleracea L.) in Vertical Farming
Authors: Semenova Natalya A. , Proshkin Yuri A. , Smirnov Alexandr A. , Dorokhov Alexey S. , Ivanitskikh Alina S. , Burynin Dmitry A. , Dorokhov Artem A. , Uyutova Nadezhda I. , Chilingaryan Narek O.
Published: October 2023

The present study has been carried out to determine the effects of four different illuminators with red, far-red, blue, and white light-emitting diodes (LEDs) on the growth, morphology, pigment composition, and chlorophyll fluorescence of spinach (Spinacia oleracea L.) of the ‘Zhirnolistny’ cultivar. We investigated these variants in two photon flux densities, 400–800 nm (PFD) 120 µmol m−2 s−1 and 180 µmol m−2 s−1. The studies were carried out in a climate chamber. Plant measurements were carried out on the 30th and 45th days of cultivation. The results showed that during the period of active growth, on the 30th day, spinach plants accumulated 2.6 and 2.4 times more fresh weight in the variant with a higher PFD (180 µmol m−2 s−1). At the end of the growing season, only a decrease in PFD had an effect on the fresh and dry weight of plants. The highest concentration of chlorophyll on both the 30th and 45th days of vegetation was found when spinach plants were grown under red-blue (RB) LEDs in a spectrum proportion of R70:B30. It was found that the variants had a higher proportion of green radiation in the spectrum of illuminators with PFD 180 µmol m−2 s−1, and the nitrate content in spinach was slightly lower than in other variants.
miR-632 Induces DNAJB6 Inhibition Stimulating Endothelial-to-Mesenchymal Transition and Fibrosis in Marfan Syndrome Aortopathy
Authors: Terriaca Sonia , Scioli Maria Giovanna , Pisano Calogera , Ruvolo Giovanni , Ferlosio Amedeo , Orlandi Augusto
Published: October 2023

Marfan syndrome (MFS) is a connective tissue disorder caused by FBN1 gene mutations leading to TGF-β signaling hyperactivation, vascular wall weakness, and thoracic aortic aneurysms (TAAs). The pathogenetic mechanisms are not completely understood and patients undergo early vascular surgery to prevent TAA ruptures. We previously reported miR-632 upregulation in MFS TAA tissues compared with non-genetic TAA tissues. DNAJB6 is a gene target of miR-632 in cancer and plays a critical role in blocking epithelial-to-mesenchymal transition by inhibiting the Wnt/β catenin pathway. TGF-β signaling also activates Wnt/β catenin signaling and induces endothelial-to-mesenchymal transition (End-Mt) and fibrosis. We documented that miR-632 upregulation correlated with DNAJB6 expression in both the endothelium and the tunica media of MFS TAA (p < 0.01). Wnt/β catenin signaling, End-Mt, and fibrosis markers were also upregulated in MFS TAA tissues (p < 0.05, p < 0.01 and p < 0.001). Moreover, miR-632 overexpression inhibited DNAJB6, inducing Wnt/β catenin signaling, as well as End-Mt and fibrosis exacerbation (p < 0.05 and p < 0.01). TGF-β1 treatment also determined miR-632 upregulation (p < 0.01 and p < 0.001), with the consequent activation of the aforementioned processes. Our study provides new insights about the pathogenetic mechanisms in MFS aortopathy. Moreover, the high disease specificity of miR-632 and DNAJB6 suggests new potential prognostic factors and/or therapeutic targets in the progression of MFS aortopathy.
Mitochondrial sAC-cAMP-PKA Axis Modulates the ΔΨm-Dependent Control Coefficients of the Respiratory Chain Complexes: Evidence of Respirasome Plasticity
Authors: Scrima Rosella , Cela Olga , Rosiello Michela , Nabi Ari Qadir , Piccoli Claudia , Capitanio Giuseppe , Tucci Francesco Antonio , Leone Aldo , Quarato Giovanni , Capitanio Nazzareno
Published: October 2023

The current view of the mitochondrial respiratory chain complexes I, III and IV foresees the occurrence of their assembly in supercomplexes, providing additional functional properties when compared with randomly colliding isolated complexes. According to the plasticity model, the two structural states of the respiratory chain may interconvert, influenced by the intracellular prevailing conditions. In previous studies, we suggested the mitochondrial membrane potential as a factor for controlling their dynamic balance. Here, we investigated if and how the cAMP/PKA-mediated signalling influences the aggregation state of the respiratory complexes. An analysis of the inhibitory titration profiles of the endogenous oxygen consumption rates in intact HepG2 cells with specific inhibitors of the respiratory complexes was performed to quantify, in the framework of the metabolic flux theory, the corresponding control coefficients. The attained results, pharmacologically inhibiting either PKA or sAC, indicated that the reversible phosphorylation of the respiratory chain complexes/supercomplexes influenced their assembly state in response to the membrane potential. This conclusion was supported by the scrutiny of the available structure of the CI/CIII2/CIV respirasome, enabling us to map several PKA-targeted serine residues exposed to the matrix side of the complexes I, III and IV at the contact interfaces of the three complexes.
Investigating the Molecular Mechanisms Underlying Early Response to Inflammation and Helicobacter pylori Infection in Human Gastric Epithelial Cells
Authors: Martinelli Giulia , Fumagalli Marco , Piazza Stefano , Maranta Nicole , Genova Francesca , Sperandeo Paola , Sangiovanni Enrico , Polissi Alessandra , Dell’Agli Mario , De Fabiani Emma
Published: October 2023

Helicobacter pylori is a leading cause of chronic gastric inflammation, generally associated with gastritis and adenocarcinoma. Activation of the NF-κB pathway mainly contributes to the inflammatory phenotype observed in H. pylori infection in humans and experimental models. Since the gastric epithelium undergoes rapid turnover, inflammation and pathogenicity of H. pylori result from early phase and chronically activated pathways. In the present study we investigated the early host response to H. pylori in non-tumoral human gastric epithelial cells (GES-1). To dissect the pathogen-specific mechanisms we also examined the response to tumor necrosis factor (TNF), a prototypical cytokine. By analyzing the activation state of NF-κB signaling, cytokine expression and secretion, and the transcriptome, we found that the inflammatory response of GES-1 cells to H. pylori and TNF results from activation of multiple pathways and transcription factors, e.g., NF-κB and CCAAT/enhancer-binding proteins (CEBPs). By comparing the transcriptomic profiles, we found that H. pylori infection induces a less potent inflammatory response than TNF but affects gene transcription to a greater extent by specifically inducing transcription factors such as CEBPβ and numerous zinc finger proteins. Our study provides insights on the cellular pathways modulated by H. pylori in non-tumoral human gastric cells unveiling new potential targets.
Radiosensitizing Effect of Dextran-Coated Iron Oxide Nanoparticles on Malignant Glioma Cells
Authors: Tran Nhan Hau , Ryzhov Vyacheslav , Volnitskiy Andrey , Amerkanov Dmitry , Pack Fedor , Golubev Aleksander M. , Arutyunyan Alexandr , Spitsyna Anastasiia , Burdakov Vladimir , Lebedev Dmitry , Konevega Andrey L. , Shtam Tatiana , Marchenko Yaroslav
Published: October 2023

The potential of standard methods of radiation therapy is limited by the dose that can be safely delivered to the tumor, which could be too low for radical treatment. The dose efficiency can be increased by using radiosensitizers. In this study, we evaluated the sensitizing potential of biocompatible iron oxide nanoparticles coated with a dextran shell in A172 and Gl-Tr glioblastoma cells in vitro. The cells preincubated with nanoparticles for 24 h were exposed to ionizing radiation (X-ray, gamma, or proton) at doses of 0.5–6 Gy, and their viability was assessed by the Resazurin assay and by staining of the surviving cells with crystal violet. A statistically significant effect of radiosensitization by nanoparticles was observed in both cell lines when cells were exposed to 35 keV X-rays. A weak radiosensitizing effect was found only in the Gl-Tr line for the 1.2 MeV gamma irradiation and there was no radiosensitizing effect in both lines for the 200 MeV proton irradiation at the Bragg peak. A slight (ca. 10%) increase in the formation of additional reactive oxygen species after X-ray irradiation was found when nanoparticles were present. These results suggest that the nanoparticles absorbed by glioma cells can produce a significant radiosensitizing effect, probably due to the action of secondary electrons generated by the magnetite core, whereas the dextran shell of the nanoparticles used in these experiments appears to be rather stable under radiation exposure.
Non-Steroidal Anti-Inflammatory Drugs Loaded to Micelles for the Modulation of Their Water Solubility
Authors: Banti Christina N. , Kalampounias Angelos G. , Hadjikakou Sotiris K.
Published: October 2023

The low water solubility of aspirin (ASPH) is well known, creating research challenges regarding both its composition and its delivery. Therefore, the development of new aspirin-based formulations that are water soluble is a research, technological, and financial issue. With the aim to improve the water solubility of ASPH, the micelle of formula SLS@ASPH (SLS = Sodium Lauryl Sulfate) was formed. The Critical Micelle Concentration (CMC) of SLS in the presence of ASPH was determined by ultrasonic velocity, complementary, and transient birefringence measurements. The SLS@ASPH was characterized by the melting point (m.p.), attenuated total reflection spectroscopy (FT-IR-ATR), and X-ray fluorescence spectroscopy (XRF) in a solid state and in a solution by ultraviolet-visible (UV-Vis) and 1H NMR spectroscopies. The SLS/ASPH molar ratio was determined to be 5/1 in SLS@ASPH. The inhibitory activity of SLS@ASPH towards lipoxygenase (LOX), an enzyme that takes part in the inflammation mechanism, was studied. The inhibitory activity of SLS@ASPH against LOX is 3.5-fold stronger than that of free SLS. The in vitro toxicity of the SLS@ASPH was tested on immortalized human keratinocyte (HaCaT) cells.
Interaction of Calmodulin with TRPM: An Initiator of Channel Modulation
Authors: Vydra Bousova Kristyna , Zouharova Monika , Jiraskova Katerina , Vetyskova Veronika
Published: October 2023

Transient receptor potential melastatin (TRPM) channels, a subfamily of the TRP superfamily, constitute a diverse group of ion channels involved in mediating crucial cellular processes like calcium homeostasis. These channels exhibit complex regulation, and one of the key regulatory mechanisms involves their interaction with calmodulin (CaM), a cytosol ubiquitous calcium-binding protein. The association between TRPM channels and CaM relies on the presence of specific CaM-binding domains in the channel structure. Upon CaM binding, the channel undergoes direct and/or allosteric structural changes and triggers down- or up-stream signaling pathways. According to current knowledge, ion channel members TRPM2, TRPM3, TRPM4, and TRPM6 are directly modulated by CaM, resulting in their activation or inhibition. This review specifically focuses on the interplay between TRPM channels and CaM and summarizes the current known effects of CaM interactions and modulations on TRPM channels in cellular physiology.
A Formal Approach to Spanish ‘Genitive’ Pronouns in Non-Nominal Domains
Authors: Mare María
Published: October 2023

This paper examines the distribution of ‘genitive’ pronouns in non-nominal domains in Spanish. These pronouns can alternate with constructions headed by the item de ‘of’ and a pronoun or other Determiner Phrases (DPs). In general Spanish, this alternation between a synthetic (nuestro ‘our’) and an analytic (de nosotros ‘of us’) option is found in the nominal domain. However, when looking at variation, this alternation appears in adverbial, verbal, and adjectival domains too. We discuss this phenomenon from a neo-constructionist approach, which assumes the late insertion of phonological exponents. We propose that the analytic and the synthetic options have almost the same syntactic structure, the only difference being the nature of the nominalizer’s φ-features. When the nominalizer values its φ-features, it can be lexicalized alone, and the ‘genitive’ pronoun lexicalizes the rest of the structure, including the introducer p/Place. Otherwise, when the nominalizer cannot (or needs not to) value its features, a ‘non-genitive’ pronoun lexicalizes the pronominal structure, and the head p/Place is lexicalized by the item de. Our proposal explains the complementary distribution between agreement/nominal morphology and the item de observed in many Spanish constructions. Different consequences are advantageously deduced.
The Depth of Cure, Sorption and Solubility of Dual-Cured Bulk-Fill Restorative Materials
Authors: Alzahrani Bashayer , Alshabib Abdulrahman , Awliya Wedad
Published: October 2023

This study aimed to examine depth of cure (DoC), mass change, water sorption and solubility of dual-cured bulk-fill restorative materials (Surfil One and Activa) in comparison with a light-cured bulk-fill composite (Filtek One Bulk-Fill) and a resin-modified glass ionomer (Fuji II LC). Twenty specimens were prepared of each material using stainless steel molds designed with a slot (8 × 4 × 2 mm) and irradiated for either 20 or 40 s. The Vickers hardness (VHN) was measured at every 0.5 mm to assess the DoC after 24 h of storage at 37 °C. The depth of cure was reported as the depth corresponding to 80% of the maximum Vickers hardness. Disc-shaped specimens were prepared of each material (n = 5) to investigate mass change, sorption and solubility after 4 months of water storage. The data were analyzed using a two-way and one-way analysis of variance (ANOVA) followed by the Tukey post hoc test (p ≤ 0.05). Fuji II LC had the greatest DoC while Activa had the lowest. The two different irradiation times did not demonstrate a significant difference in DoC for all dual-cured materials (p > 0.05). Fuji II LC had the highest sorption while Filtek One showed the lowest. Surefil One and Fuji II LC had a negative solubility. This study concluded that dual-cured materials showed different depth of cure values despite having the same setting reaction. Both materials exhibited a high water sorption, which might jeopardize their dimensional stability and effect their clinical performance.