bluuebunny/crossref_metadata_2025 huggingface dataset:
Salud Mental Global: Enfoque, Crisis e Impacto
Authors: Bustamante Inés , Yamaguchi Sakiko
Published: November 2024

Objetivos: Reflexionar sobre la evolución de los enfoques de Salud Mental Global (SMG) en los últimos quince años y su aplicación a políticas y servicios, particularmente en Latinoamérica. Describir y explicar las crisis mundiales que la han impactado y los desafíos que representan para los países de la subregión. Temas abordados: A medida que los discursos y enfoques en SMG evolucionaron para centrarse más en los determinantes sociales de la salud mental y materializar los Objetivos de Desarrollo Sostenible, las comunidades cobraron creciente importancia en los sistemas de salud mental de América Latina. Sin embargo, crisis globales como la pandemia COVID-19 y el cambio climático demostraron que la salud mental de las poblaciones vulnerables, incluidas las personas con enfermedades mentales y las poblaciones de zonas rurales e indígenas, se vieron desproporcionadamente afectadas, exacerbando las desigualdades ya existentes en el acceso a atención y servicios adecuados. Por otro lado, la cultura es un factor crítico a considerarse en las respuestas locales al sufrimiento y a procesos de sanación ante las crisis. Conclusiones: Existe la necesidad de priorizar la equidad e inclusión en el cuidado de la SMG. Las crisis globales y regionales que la afectan significativamente hacen de las personas y comunidades afectadas una valiosa fuente de conocimiento e información para el desarrollo de enfoques y prácticas innovadoras que promuevan un acceso equitativo a la atención de salud. Es por ello muy importante reconocer sus formulaciones y planteamientos.
Entidades psiquiátricas frecuentes y perspectivas clínicas en tiempos de crisis
Authors: Vargas-Murga Horacio , Vargas-Suárez Kenneth
Published: November 2024

El presente artículo revisa las entidades psiquiátricas frecuentes y las perspectivas clínicas durante tiempos de crisis,analizando su impacto en la salud mental. Las crisis, clasificadas en pandemias, desastres naturales, conflictos armados y crisis económicas, aumentan significativamente la prevalencia de trastornos mentales y exacerban condiciones preexistentes, desafiando los alcances y posibilidades de los sistemas de salud mental. La pandemia de COVID-19, por ejemplo, ha incrementado las hospitalizaciones psiquiátricas en niños y adolescentes debido al uso excesivo de medios digitales. El artículo analiza diversos trastornos psiquiátricos, como psicosis, cuadros afectivos, ansiedad, trastornos del sueño, alimentarios, obsesivo-compulsivo y relacionados con el estrés. Se puntualizan definiciones, síntomas, factores de riesgo, impactos durante períodos de crisis y estrategias de tratamiento. Destaca la importancia del apoyo social para mitigar los efectos negativos de las crisis en la salud mental, recomendando redes comunitarias sólidas y servicios accesibles. Además, sugiere integrar tecnología, como aplicaciones móviles y telemedicina, para ofrecer apoyo y tratamiento psicológico en circunstancias de crisis. Se enfatiza la necesidad de un enfoque multidimensional y adaptativo para un manejo eficaz, promoviendo una respuesta más efectiva y resiliente del sistema de salud mental. Se resalta asimismo la importancia de fortalecer estos sistemas y desarrollar estrategias terapéuticas adaptativas para enfrentar las necesidades emergentes en épocas de crisis.
A Qualitative Study of Information Needs about Human papillomavirus (HPV) Among Elementary School Students
Authors: Syahrani Dilla , Mutiar Astri , Marfuah Dewi , Purnama Heni
Published: November 2024

Aims: This research aims to to identify the information requirements of elementary school children toward human papillomavirus (HPV).
Methods: The researcher employed a qualitative method utilizing the focus group discussion technique to gather data on several predetermined topics. A total of 20 female students who met the criteria were divided into several groups, and the discussions were recorded and documented.
Result: Most of elementary school children have no background knowledge of HPV. Many of them have limited understanding of sexual health education. Although children aged 9-14 have received the HPV vaccine, most of them do not know about HPV. Further information is needed regarding the definition of HPV, the virus transmission process, potential symptoms, diseases caused by the virus, and complications arising from inadequate attention to prevention.
Conclution: Providing comprehensive and child-friendly information about the HPV virus is essential to enhance the knowledge of elementary school students. It also contributes to increasing the acceptance of the HPV vaccine and maximizing preventive measures.
The Effect of Hardiness Training on Academic Burnout in Nursing Students in STIKep PPNI Jawa Barat
Authors: Sudrajat Diwa Agus , Rosmayanti Wawat Wati , Kumala Tria Firza
Published: November 2024

Aims: Academic burnout in nursing students is a result of academic demands, lack of interest, and incompetence, necessitating immediate action and hardiness training.
Method: This study surveyed 50 nursing students with academic burnout using the MBI-SS questionnaire. The sample was randomly selected, and statistical tests included Wilcoxon nonparametric and Mann- Whitney.
Results: There is a significant difference or influence on the scores before and after being given hardiness training with the Wilcoxon test results in the intervention group (Asymp. Sig. (2-tailed) = 0.000), there is an influence on the results of the Mann Whitney test after being given hardiness training in the intervention and control groups with the data results being (Asymp. Sig. (2-tailed) 0.002), which means that this research has a significant influence-because the Asymp. Sig. (2-tailed) < 0.05.
Conclusion: Providing Hardiness training can reduce the level of academic burnout in nursing students.
Relationship Between Early Complex Food Practice with Nutritional Status Among Children Age 7 to 23 Months
Authors: Megasari Megasari , Mariyani Mariyani
Published: November 2024

Aims:: Knowing the factors associated with the practice of early complementary feeding with the nutritional status of infants aged 7 to 23 months in Sipayung Village, Cipanas District, Lebak Regency.
Method: A cross-sectional design was used for quantitative and analytical study. A purposive sampling method was used to choose 51 mothers with babies between the ages of 7 and 23 months as the study sample. A questionnaire was used as the study tool. The square test was used to look at the primary data.
Result: The single-variable study showed that 82.4% of infants had good nutrition and 72.5% of those who were given MP ASI did so correctly. The bivariate analysis showed that there was a link between the number of times MP-ASI was given, the age at which it was first given, how often it was given, how consistently it was given, and the infant's nutritional status (p value = 0.000).
Conclusion: The practice of giving MP-ASI has to do with how well babies ages 7 to 23 months are eating. Mothers of babies should always pay attention to how, how often, and how much they feed their babies as they get older. This includes the form, frequency, and amount of food. The mother of the baby can also regularly weigh her child at the posyandu
Effectiveness of Health Education Using Booklet & Video on Knowledge of Pregnancy Danger Signs Among Primigravida Mothers
Authors: Khairah Rahayu , Dewi Linda , Aliyah Nur , Nurfikah Nunung
Published: November 2024

Aims : The aims of this study was to see the effectiveness of providing pregnancy danger sign videos and education through KIA books on the knowledge of pregnant women about pregnancy danger signs.
Methods : The study conducted in May-June 2023, involved 180 pregnant women, 90 receiving video intervention on pregnancy danger signs and 90 receiving MCH book education, and used a questionnaire for bivariate analysis.
Results : Mann-Whitney test in the video group are Sig.2-tailed of 0.000 <0.05, then the provision of educational videos is effective in increasing the knowledge of pregnant women about the danger signs of pregnancy and statistically significant.
Conclusions : The Mann-Whitney test showed that educational videos effectively increase pregnant women's knowledge about danger signs of pregnancy, while MCH book education was found to be insignificant. The results suggest that educational videos are effective in raising awareness.
Relationship Between Knowledge and History of Low Birth Weight with the Incidence of Stunting Among Toddlers in Public Health Center
Authors: Purwati Wilia , Sutarno Maryati
Published: November 2024

Aims: To determine the relationship between knowledge and historical records of low birth weight (LBW) and the prevalence of stunting in young children at the Cibadak Community Health Center in Lebak Regency.
Method: The study used incidental sampling, involving women with toddlers at the Cibadak Community Health Center, to evaluate their health, using SPSS 22, chi square, and Spearman rank tests.
Results: The results of the research show that knowledge and history of LBW in toddlers have a significant relationship. The p value from the chi square test on the history of LBW with stunting has a value of 0.000, as well as the p value in the Spearman rank test on the mother's knowledge variable regarding the incidence of stunting also has a value of 0.000.
Conclusions: The study focuses on the link between awareness and medical history of low birth weight and stunting in toddlers, emphasizing the necessity of nutritional programs and attention from healthcare providers.
The Use of Social Media on Self-Concept in Adolescents: A Multiple Case Study
Authors: Purnama Heni , Fitriana Anisa Nur
Published: November 2024

Aims: Adolescent self-concept plays a role so that adolescents can adjust to their environment, adolescent self-concept can be influenced by the use of social media. Social media can have a positive or negative influence on adolescent self-concept.
Method: A qualitative research methodology was utilized with multiple case study design. Data collection using structure interview. The subjects in this study were 2 teenagers who active using social media. This research was conducted in the Bandung area.
Results: Teenagers use social media to express honesty, share photos, and share daily lives. However, excessive use negatively impacts self-concept, productivity, and empathy, leading to distractions like forgetting study time and helping parents clean.
Conclusion: Social media can affect self-concept in adolescents depending on the use made by adolescents, on social media adolescents get a negative or positive self-concept.
The Effect of Animation Video-Based Education Toward Stunting Knowledge and Attitude Among Female Adolescents in Jakarta
Authors: Donana Yurongki , Nurhayati Nurhayati , Endah P Tria Astika , Hadiyani Wini
Published: November 2024

Aims: The prevalence of stunting in Indonesia is one of the crucial problems that will have an impact on the next generation, so it needs to be prevented. Increasing stunting prevention knowledge and attitudes in adolescents related to health investment in the first 8000 days of life is important in reducing stunting rates. The aims of the study to identify the effect of investment education for the first 8000 days of life with animated videos as an effort to break the stunting cycle.
Methods: This study used the Quasi Experiment method with a two-group pre-posttest design with 214 respondents divided into an intervention group and a control group with 107 people in each group. The research analysis used Wilcoxon Rank and Manova.
Results: This study after being given the intervention showed that there were significant differences in the knowledge and attitude scores between the control group and the intervention group with a p-value of 0.000 (p <α 0.05).
Conclusion: This study shows that providing education on health investment in the first 8000 days of life with animated videos affects increasing knowledge and attitudes of young women in preventing stunting.
Evaluating the Impact of Semi-Immersive Virtual Reality on Urinary Catheterization Skills, Motivation, and Satisfaction in Nursing Students
Authors: Apriyanto Bagus Sholeh , Akasyah Wildan , Wahyuni Sri , Rias Yohanes Andy
Published: November 2024

Aims: The basic core skill and competency nursing students need is urinary catheterization. Semi-immersive virtual reality (SIVR) based research innovations based on situational learning theory have not been widely used to improve student proficiency. This study aimed to analyze the skills, motivation, and satisfaction of nursing students with urinary catheterization learning before and after the implementation of SIVR.
Methods: The study was designed as a pre-test and post-test randomized controlled trial (RCT). The study population was undergraduate nursing students at the Bhakti Wiyata Health Sciences Institute, Kediri. The SIVR group (n=52) received catheterization skill practice through SIVR, whereas the control group (n=52) was given 2-dimensional video learning. The results obtained were catheterization skills, motivation, and satisfaction, which were evaluated before and after the intervention. In this study's statistical test analysis, the chi-square test and the independent t-test were used.
Results: Post-test results showed a significant improvement in catheterization skills in the SIVR group (M = 92.74, SD = 1.05, p = 0.032) compared to the control group (M = 79.95, SD = 1.51), a significant improvement in motivation in the SIVR group (M = 6.48, SD = 0.54, p = 0.010) compared to the control group (M = 5.23, SD = 0.49), and a significant improvement in satisfaction in the SIVR group (M = 3.49, SD = 0.83, p = 0.024) compared to the control group (M = 2.88, SD = 0.52).
Conclusion: SIVR simulation and video learning can improve nursing students' skills, motivation, and satisfaction. SIVR program learning can be recommended as an intervention for the effectiveness of the quality of practical learning following current technological advances and in the long term it is expected to improve graduates' practical skills.
The Effect of Combined Foot Massage and Nature Music on the Sleep Quality of Critically Ill Patients
Authors: Nurhayati Nunung , Waluyo Agung , Kariasa I Made , Asih Sali Rahadi
Published: November 2024

Aims: Critical patients in ICU and HCU often experience poor sleep due to excessive stress and various symptoms like environmental factors, pain, noise, anxiety, and physical discomfort. Foot massage and natural music interventions can help meet their sleep needs, providing a relaxing effect and improving physical and psychological conditions. The purpose of this study is to determine the effect of providing combination therapy of foot massage and natural music therapy in Dr. Hospital's peacock room Salamun 2023.
Methods:  This type of research is pre-experimental with a pre-post test of one group and uses the Richard Champbell Sleep Questionnaire (RCSQ). Then the data was processed using a paired sample T-test. The sample in this study is 16 respondents.
Results: The results of the statistical test obtained a p-value of 0.01, meaning that there was an effect between the pretest and posttest of the combination of foot massage and natural music therapy on the quality of sleep in critical patients in the peacock room at Dr. Salamun Hospital, Bandung City, 2023.
Conclusions : the conclusion of this study is that there is an effect of foot massage therapy and natural music on the sleep quality of critical patients as shown by the results of measuring the quality of critical patients before and after consecutive interventions with massage of both feet combined with natural music. For 10 minutes it has been proven to be able to improve sleep quality in critically ill patients so that this intervention can be applied as a therapy to improve sleep quality.
Gender Equality. Conditions for Ensuring Gender Equality in Education
Authors:   Alexandru Ioan Cuza University, Cojocaru Stefan , Bunea Ovidiu ,   Alexandru Ioan Cuza University, Cojocaru Daniela ,   Alexandru Ioan Cuza University, Neculau Catalina , Patrascu Alin Adrian
Published: November 2024

This study focuses on the concept of gender equality in education, examining the role of national and European Union legislation, as well as international frameworks like the United Nations' CEDAW, in promoting gender parity. The research analyzes the structural causes of gender inequalities and discrimination, highlighting how societal norms and individual stories about gender contribute to these disparities. Special attention is given to barriers in achieving educational equity, considering both access to education and the learning process itself. Additionally, the study explores the significance of parental education programs, addressing gender perspectives in their development and implementation. It examines the gender dynamics within parental education, including the gender of educators, participants, and the challenges of measuring the impact of such programs. Through a thorough review of relevant policies and strategies, the study suggests practical measures to enhance gender equality in education, advocating for the continuous promotion of gender-sensitive practices in both school curricula and extracurricular activities. The findings emphasize the need for greater attention to gender equality in educational policies and the importance of fostering an inclusive environment for both women and men in Romania and across the European Union.
Development of The Tanjung Pandan Old Town Area as a Heritage and Gastronomy Tourist Attraction
Authors: Pratiwi Yunisti , Wahyuni Eka Indah
Published: November 2024

The purpose of study This is for know history and philosophy and tradition Culture Good in the form of reserve culture and food traditional, as well For know How strategy development so that become Power pull Heritage and Gastronomy Tourism in Tanjung Pandan City. The type of research used is qualitative. Research results This will describe history and the philosophy and development strategies implemented with hope existence effort This Can make become Tourist Attraction​ especially heritage and gastronomy which are interesting and in demand by tourists.
Strengthening Tourism Products Through Storytelling Exploration in Kemiriombo, Gerbosari Village, Samigaluh District, Kulonprogo Regency
Authors: Iban Carlos , Marsono  , Fatkurrohman  , Nuryani Anik , Rahayuningsih Handayani , Bayu Aji Khusnul
Published: November 2024

Kemiriombo, a hamlet in Gerbosari Village, Kulon Progo, Yogyakarta, is known for its diversity of spices (empon-empon) cultivated and processed by the local community. This area has significant potential for developing tourism based on local culture and traditions, but the tourism narrative has not been optimally managed. This study aims to explore the potential of storytelling as a strategy for developing tourist destinations in Kemiriombo. The methods used include observation, interviews, Focus Group Discussions (FGD), surveys, and community participation to understand the needs and expectations of the local community. The study found that Kemiriombo has tourism potential such as the processing of empon-empon at Rumah Jamu Menoreh, local cultural arts such as Jathilan, Gejog Lesung, and Karawitan, as well as culinary traditions like Sega Brongsong and Legondo. The 5W1H technique (Who, What, When, Where, Why, How) was applied in the storytelling analysis to produce engaging narratives that can promote local cultural identity. Storytelling plays a crucial role in introducing local products and traditions, enhancing tourist attraction, and preserving community culture. Through the development of strong narratives, Kemiriombo is expected to become a tourist destination that offers not only visual experiences but also emotional and social dimensions for visitors. The integration of local traditions and storytelling innovation is expected to positively impact the welfare of the local community.
FROM MANGA LITERATURE TO LIVE-ACTION FILM/TV: MACKENYU'S CROSS-CULTURAL ODYSSEY IN GLOBAL CINEMA
Authors: Ding Ji
Published: November 2024

This research examines the career of Japanese-American actor Mackenyu Arata, particularly his roles in live-action adaptations of manga and anime, to understand his influence on cross-cultural narrative integration in cinema. The study focuses on Mackenyu's portrayal of leading and important supporting characters in several high-profile adaptations, including Netflix's One Piece, where he plays Zoro, and others such as Chihayafuru, JoJo's Bizarre Adventure, Rurouni Kenshin, and Fullmetal Alchemist. Employing qualitative analysis, this paper investigates how Mackenyu strategically navigates away from the shadow of his father, legendary actor Sonny Chiba, to forge an independent identity and career. Data was collected through reviews, audience reception studies, and box office performance, and analyzed to assess thematic consistency and cultural impact across different markets. Experts in film adaptation theory, such as Linda Hutcheon, and resources like The Routledge Companion to World Cinema provided theoretical frameworks for analyzing how narratives are adapted for global audiences and the role of actors in these adaptations. The findings reveal that Mackenyu's choices are not merely artistic but are also deliberate efforts to blend Eastern and Western cinematic traditions, enhancing his appeal as a global actor. His roles often reflect a profound engagement with his bicultural heritage, challenging the notion that he is only a "second-generation star." These roles resonate with global audiences and contribute to the ongoing discourse on cultural integration in film. The study concludes that Mackenyu is a key figure in the realm of live-action adaptations, with a unique ability to transcend cultural and cinematic boundaries.
B. subtilis biofilm as a cybernetic system
Authors: Blaznik Mojca , Stopar David
Published: November 2024

Here, we view biofilm as a nested cybernetic system that cannot be studied in isolation from the dynamics in the rest of the ecosystem. We provide a systems view of B. subtilis development from the inoculation to hibernation. We propose that B. subtilis biofilm development in an aqueous environment is a temporal response to changes in the ecosystem provoked and caused by bacteria. We show that the initial bacterial growth changes physio-chemical parameters of the ecosystem, which in turn trigger the initiation of the biofilm formation and guide its development, structure, and ultimately its decay and dispersion. Formation of the biofilm is just one of the possible outputs of the bacterial cybernetic system. It is not invariably the best response that fits all environmental needs. Viewing biofilm as a cybernetic set of interrelated objects, capable of receiving, storing, processing, and exchanging information with the rest of the ecosystem, should become an integral part of biofilm studies.
Implementing an adaptive reuse on industrial buildings: a proposal for transforming Erbil’s Silo in Kurdistan-Iraq into a hotel
Authors: Alfaris Seema Khalid , Rafeeq Dalia Ali , Sami Sanar Sardar , Ahmed Raghad Dlzar
Published: November 2024

Industrial buildings, serving as markers of cultural heritage, connect our cultural identity from the past through the present and into the future. Unfortunately, many of these buildings lose their identity when they are converted into another function. Through the case study analysis (the Silo of Erbil City), a qualitative method is employed to examine design strategies and spatial transformations in recent adaptive reuse projects of existing structures. Through the literature review, this research investigates the importance of adaptive reuse and heritage buildings, underscoring their historical and theoretical underpinnings, and subsequently examines contemporary approaches to architectural criteria towards habitation for existing structures in the public, creative, and cultural domains. The study findings identify common fundamental elements of industrial heritage adaptive reuse and innovative design strategies applied in recent adaptive reuse projects, highlighting the potential to transform neglected or vacant abandoned buildings into urban open spaces. The study uses a comprehensive methodology involving case study analyses and diverse data collection techniques. The case study properties are the structural systems, natural lighting, and the surrounding open spaces. As a result, a hotel with a multipurpose hall, and a museum dedicated to the history of the Silo have been proposed, aiming at conserving the valuable heritage in this industrial area as well as providing an alternative perspective for the adaptive reuse of industrial structures in Erbil city. Finally, the implications of the research for the economic and social dimensions of urban development lie in understanding and promoting sustainable preservation strategies. This work is considered the first step for future research in Iraq, specifically in Kurdistan Region. The research concludes that the Silo of Erbil is one of the successful examples of the process of reusing buildings. It also concludes that less intervention in reusing a building can protect the building identity.
Deciphering transcriptome patterns in porcine mesenchymal stem cells promoting phenotypic maintenance and differentiation by key driver genes
Authors: Khaveh Nadia , Buschow René , Metzger Julia
Published: November 2024

Mesenchymal stem cells (MSC) are fibroblast-like non-hematopoietic cells with self-renewal and differentiation capacity, and thereby great potential in regeneration and wound healing. MSC populations are heterogeneous not only inherently, but also among different model species. In particular, porcine MSC serve as a frequently used resource for translational research, due to pigs’ distinctive closeness to human anatomy and physiology. However, information on gene expression profiles from porcine MSC and its dynamics during differentiation is sparse, especially with regard to cell surface and inner cell markers. In this study, we investigated the transcriptome of bone marrow-derived MSC and its differentiated cell types in a minipig breed for experimental research, known as Mini-LEWE, using bulk mRNA sequencing. Our data highlighted Rap1 signaling and downstream pathways PI3K-Akt and MAPK signaling as potential players for the maintenance of stemness of BM-MSC. In addition, we were able to link the process of differentiation to changes in the regulation of actin cytoskeleton. A total of 18 “BM-MSC differentiation driver markers” were identified, potentially promoting the process of differentiation into adipocytes, chondrocytes as well as osteocytes. Our results offer a new perspective on the molecular phenotype of porcine BM-MSC and the transcriptional responses in new differentiated progeny.
Combining external physical stimuli and nanostructured materials for upregulating pro-regenerative cellular pathways in peripheral nerve repair
Authors: Redolfi Riva Eugenio , Özkan Melis , Stellacci Francesco , Micera Silvestro
Published: November 2024

Peripheral nerve repair remains a major clinical challenge, particularly in the pursuit of therapeutic approaches that ensure adequate recovery of patient’s activity of daily living. Autografts are the gold standard in clinical practice for restoring lost sensorimotor functions nowadays. However, autografts have notable drawbacks, including dimensional mismatches and the need to sacrifice one function to restore another. Engineered nerve guidance conduits have therefore emerged as promising alternatives. While these conduits show surgical potential, their clinical use is currently limited to the repair of minor injuries, as their ability to reinnervate limiting gap lesions is still unsatisfactory. Therefore, improving patient functional recovery requires a deeper understanding of the cellular mechanisms involved in peripheral nerve regeneration and the development of therapeutic strategies that can precisely modulate these processes. Interest has grown in the use of external energy sources, such as light, ultrasound, electrical, and magnetic fields, to activate cellular pathways related to proliferation, differentiation, and migration. Recent research has explored combining these energy sources with tailored nanostructured materials as nanotransducers to enhance selectivity towards the target cells. This review aims to present the recent findings on this innovative strategy, discussing its potential to support nerve regeneration and its viability as an alternative to autologous transplantation.
Strain-level genomic analysis of serotype, genotype and virulence gene composition of group B streptococcus
Authors: Zeng Zhen , Li Meng , Zhu Simin , Zhang Ke , Wu Yifan , Zheng Minzi , Cao Yang , Huang Zhenyu , Liao Qinping , Zhang Lei
Published: November 2024

Introduction

GBS (group B streptococcus) is an opportunistic pathogen that can colonize healthy individuals but presents significant challenges in clinical obstetrics and gynecology, as it can cause miscarriage, preterm birth, and invasive infections in newborns. To develop specific and personalized preventative strategies, a better understanding of the epidemiological characteristics and pathogenic features of GBS is essential.

Methods

We conducted a comprehensive strain-level genomic analysis of GBS, examining serotype and genotype distributions, as well as the composition and correlations of virulence genes using the blastn-short mode of the BLAST program(v2.10.0+), mlstsoftware (https://github.com/tseemann/mlst), Snippy (v4.6.0), FastTree (v2.1.11) and iTOL. The coding sequence region of virulence factors was annotated by Prodigal (v2.6.3) and Glimmer(v3.02b). We further identified host protein interacting with Srr2 by mass spectrometry analysis.

Results

While certain genotypes showed strong serotype consistency, there was no significant association between overall serotypes and genotypes. However, the composition of virulence genes was more closely related to the phylogeny of GBS, among which simultaneous presence of Srr2 and HygA exhibit significant association with hypervirulence. Tubulin emerged as the most distinct and abundant hit. The specific interaction of Tubulin with Srr2-BR, rather than Srr1-BR, was further confirmed by immunoblotting.

Discussion

Considering the impact of cytoskeleton rearrangement on GBS pathogenesis, this observation offers a plausible explanation for the hypervirulence triggered by Srr2. Collectively, our findings indicate that in the future clinical practice, virulence gene detection should be given more attention to achieve precise GBS surveillance and disease prevention.
Data management practice of health extension workers and associated factors in Central Gondar Zone, northwest Ethiopia
Authors: Melaku Mequannent Sharew , Yohannes Lamrot
Published: November 2024

Introduction

Generating quality data for decision-making at all levels of a health system is a global imperative. The assessment of the Ethiopian National Health Information System revealed that health information system resources, data management, dissemination, and their use were rated as “not adequate” among the six major components of the health system. Health extension workers are the frontline health workforce where baseline health data are generated in the Ethiopian health system. However, the data collected, compiled, and reported by health extension workers are unreliable and of low quality. Despite huge problems in data management practices, there is a lack of sound evidence on how to overcome these health data management challenges, particularly among health extension workers. Thus, this study aimed to assess data management practices and their associated factors among health extension workers in the Central Gondar Zone.

Method

An institution-based cross-sectional study was conducted among 383 health extension workers. A simple random sampling method was used to select districts, all health extension workers were surveyed in the selected districts, and a structured self-administered questionnaire was used for data collection. The data was entered using EpiData version 4.6 and analyzed using STATA, version 16. Bivariable and multivariable binary logistic regression analyses were executed. An odds ratio with a 95% confidence interval and a p-value of <0.05 was calculated to determine the strength of the association and to evaluate statistical significance, respectively.

Results

Of the 383 health extension workers enrolled, all responded to the questionnaire with a response rate of 100%. Furthermore, 54.7% of the respondents had good data management practices. In the multivariable logistic regression analysis, being a married woman, having good data management knowledge, having a good attitude toward data management, having 1–5 years of working experience, and having a salary ranging from 5,358 to 8,013 Ethiopian Birr were the factors significantly associated with good data management practices among health extension workers. The overall data management practice was poor with only five health extension workers out of ten having good data management practices.
Internal adaptation assessment of implant infrastructures manufactured through five different techniques (heat-press, milling, lost wax, calcinable cylinder, and CAD/Waxx®): an in vitro pilot study
Authors: Sartori Régis , Fernandes Juliana Campos Hasse , Fernandes Gustavo Vicentis Oliveira , Joly Julio Cesar
Published: November 2024

Introduction

The aim of this study was to evaluate the fit performance of implant infrastructures manufactured by five different techniques: heat-press (IPS), milling (ZIR), lost wax (CER), calcinable cylinder (CAL), and CAD/Waxx® (CAD).

Methods

The methodology was based on the Replica Technique, which can simulate and evaluate the fit of the infrastructure on the implant component. Thus, each infrastructure was internally filled with low-viscosity silicone addition and seated on the component until its final setting, obtaining the replica of the cementation space. After removing the coping containing the silicone film, light-density silicone was inserted addition in place of the components, and in its surroundings, condensation silicone was applied, establishing support for the assembly. The joint was sectioned mesiodistally, photographed, and analyzed in image processing software in order to measure the thickness of the interface infrastructure/implant at five different areas: marginal opening (M), gingival-axial angle (G-A), axial region (A), axial-occlusal angle (A-O) and occlusal surface (O).

Results

The lowest and the highest average thickness between groups was, respectively, IPS: 187.5 μm and CAD: 221.6 μm, with statistically significant differences (p < 0.01) among all five groups; the lowest and the highest average of all groups in each point was, respectively, A: 99.86 μm and O: 279.78 μm. The IPS group exhibited the lowest value of the internal space of the infrastructure on the implant. The marginal region of all groups demonstrated a correlation with the findings in the literature, except the CAL group; otherwise, the occlusal region and the angles A-O and G-A exhibited values beyond that expected.

Discussion

It was possible to conclude that the five infrastructure groups presented different adaptations, suggesting possible interference in the internal spaces due to the manufacturing infrastructure processes.
Comparative study on pregnancy complications: PGT-A vs. IVF-ET with gender-specific outcomes
Authors: Guo Ling , Li Xiao , Guo Anliang , Wang Yufeng , Liang Yue , Li Yan , Xu Xinbo , Lv Hong
Published: November 2024

The safety and clinical effectiveness of preimplantation genetic testing for aneuploidy (PGT-A) in improving pregnancy outcomes for sub-fertile patients remains controversial. Potential sex-based differences in the relationship between PGT-A and pregnancy complications have not been investigated, which could guide the appropriate clinical application of PGT-A. In this secondary analysis of data from a multicenter, randomized, controlled, non-inferiority trial (NCT03118141), 940 women who achieved singleton live birth during the trial were included to estimate the between-group differences in pregnancy complications following PGT-A versus conventional in vitro fertilization (IVF) vary with fetal sex. Logistic regression analysis was used to adjust for possible confounders, and subgroup analysis was also performed. Among male fetuses, the risk of maternal preeclampsia was significantly lower after PGT-A compared to conventional IVF treatment (3.37% vs. 7.88%; adjusted OR, 0.40; 95% CI, 0.17-0.92; P = 0.032). However, this protective effect was not observed in pregnancies with female fetuses (3.63% vs. 3.38%; adjusted OR, 1.04; 95% CI, 0.36-3.00; P = 0.937). In addition, no significant sex-dependent differences in the risks of other pregnancy complications or neonatal outcomes were detected between PGT-A and conventional IVF groups (P > 0.05). In summary, PGT-A was associated with a decreased risk of maternal preeclampsia in singleton pregnancies with male fetuses, highlighting its potential utility in preeclampsia prevention in addition to spontaneous abortion rate reduction.
Efficacy of acupuncture for lumbar disc herniation: changes in paravertebral muscle and fat infiltration – a multicenter retrospective cohort study
Authors: Yan Liang , Zhang Jiliang , Wang Xianliang , Zhou Qinming , Wen Jingdong , Zhao Haihong , Guo Kai , Zeng Jianhua
Published: November 2024

Objective

This study seeks to elucidate the dynamic alterations in the multifidus, erector spinae, and psoas major muscles, along with their fatty infiltration, in patients diagnosed with lumbar disc herniation treated through acupuncture. Concurrently, the Visual Analogue Scale (VAS) and Japanese Orthopedic Association (JOA) scores are employed to evaluate modifications in lumbar and leg pain and the enhancement in lumbar functionality.

Methods

A retrospective multi-center cohort study enrolled 332 adult LDH patients. Participants were divided into acupuncture and rehabilitation therapy groups. The acupuncture cohort received targeted treatments at specific acupuncture points, while the rehabilitation group received traditional rehabilitative therapy. Magnetic Resonance Imaging (MRI) gauged muscle cross-sectional areas (Sm, Se, Sp) and their ratios to vertebral area (Sm/Sv, Se/Sv, Sp/Sv), and fatty infiltration areas (Sfm, Sfe, Sfp) and their ratios (Sfm/Sv, Sfe/Sv, Sfp/Sv). Pain and function were assessed using Visual Analogue Scale (VAS) and Japanese Orthopedic Association (JOA) scores pre-treatment, 2-weeks, and 3-months post-intervention.

Results

A total of 332 patients were enrolled for analysis. Post-treatment, the acupuncture group exhibited increased Sm, Se, Sp and their ratios and reduced fatty infiltration areas and their ratios (P<0.05) compared to rehabilitation. Both treatments decreased VAS scores and enhanced JOA scores at both intervals (P<0.05). Intriguingly, no significant disparities were observed between the acupuncture and rehabilitation groups concerning pain and JOA scores at the 2-week follow-up (p>0.05); however, 3 months post-treatment, the acupuncture group significantly outperformed the rehabilitation group in both pain and JOA scores (p<0.05).

Conclusion

This study demonstrates that acupuncture treatment is significantly more effective than traditional rehabilitation therapy in improving paraspinal muscle function, reducing muscle fat infiltration, and alleviating lumbar and leg pain in patients with lumbar disc herniation (LDH). Specifically, acupuncture significantly increases the cross-sectional areas (Sm, Se, Sp) of the paraspinal muscles and reduces muscle fat infiltration, showing superior long-term results in pain relief and functional improvement. Future research should further explore the long-term effects of acupuncture on the function and structure of paraspinal muscles, assess its potential in preventing the recurrence of LDH, and delve deeper into how acupuncture affects paraspinal muscles at the molecular level, to better understand its therapeutic mechanisms and enhance its clinical application.
Challenges in tendon–bone healing: emphasizing inflammatory modulation mechanisms and treatment
Authors: Jiang Fan , Zhao Haibo , Zhang Po , Bi Yanchi , Zhang Haoyun , Sun Shenjie , Yao Yizhi , Zhu Xuesai , Yang Fenghua , Liu Yang , Xu Sicong , Yu Tengbo , Xiao Xiao
Published: November 2024

Tendons are fibrous connective tissues that transmit force from muscles to bones. Despite their ability to withstand various loads, tendons are susceptible to significant damage. The healing process of tendons and ligaments connected to bone surfaces after injury presents a clinical challenge due to the intricate structure, composition, cellular populations, and mechanics of the interface. Inflammation plays a pivotal role in tendon healing, creating an inflammatory microenvironment through cytokines and immune cells that aid in debris clearance, tendon cell proliferation, and collagen fiber formation. However, uncontrolled inflammation can lead to tissue damage, and adhesions, and impede proper tendon healing, culminating in scar tissue formation. Therefore, precise regulation of inflammation is crucial. This review offers insights into the impact of inflammation on tendon–bone healing and its underlying mechanisms. Understanding the inflammatory microenvironment, cellular interactions, and extracellular matrix dynamics is essential for promoting optimal healing of tendon–bone injuries. The roles of fibroblasts, inflammatory cytokines, chemokines, and growth factors in promoting healing, inhibiting scar formation, and facilitating tissue regeneration are discussed, highlighting the necessity of balancing the suppression of detrimental inflammatory responses with the promotion of beneficial aspects to enhance tendon healing outcomes. Additionally, the review explores the significant implications and translational potential of targeted inflammatory modulation therapies in refining strategies for tendon–bone healing treatments.
Predictive analysis of osteoarthritis and chronic pancreatitis comorbidity: complications and risk factors
Authors: Halabitska Iryna , Petakh Pavlo , Oksenych Valentyn , Kamyshnyi Oleksandr
Published: November 2024

Background

The comorbidity of chronic pancreatitis (CP) in patients with osteoarthritis (OA) is insufficiently studied, and the reciprocal impact of these conditions remains poorly understood. This study aimed to investigate potential predictors for the development of CP in OA patients, as well as associated complications.

Methods

A cohort of 181 patients was categorized into four groups: a control group (n=30), patients with OA (n=68), patients with CP (n=31), and patients with OA and comorbid CP (n=52). All four groups had no statistical differences in age and gender. The study utilized the WOMAC index, Visual Analog Scale (VAS), Lequesne index, biochemical assays, and advanced statistical methods to assess joint status in OA patients with comorbid CP. It explored potential predictors of comorbidity development and associated complications.

Results

The study revealed that concurrent CP in OA exacerbates progression and contributes to malnutrition. Body Mass Index (BMI) emerged as a potential predictor for CP comorbidity development in OA patients. Factors such as the WOMAC total score, fecal elastase-1, C-reactive protein (CRP), ferritin, retinol, tocopherol, 25-hydroxyvitamin D3, and BMI were found to influence the development of comorbidity of CP in OA. Additionally, Gastrointestinal Symptom Rating Scale-Diarrhea Syndrome (GSRS-DS), Gastrointestinal Symptom Rating Scale-Constipation Syndrome (GSRS-CS), Qualitative Assessment of the Symptoms and Impact of Pancreatic Exocrine Insufficiency Domain A (PEI-Q-A), retinol, tocopherol, and iron were identified as potential predictors comorbidity CP with exocrine pancreatic insufficiency in OA patients.

Conclusion

The presence of CP in OA patients exacerbates disease progression and complications, necessitating further investigation.
Examining pathogen avoidance in predator-prey and scavenging systems
Authors: Clapp Justin G. , Malmberg Jennifer L. , Holbrook Joseph D.
Published: November 2024

That predators ‘cull the sick and the weak’ is an adage in ecological texts, but the mechanisms by which disease is curtailed within ecosystems has puzzled ecologists for many years. Advances in our understanding of host-pathogen interactions have revealed defense mechanisms implemented by hosts that minimize infectious diseases in wild populations. Defense mechanisms for hosts include adaptations that ameliorate fitness loss or preemptively limit pathogen exposure, and these mechanisms underlie fundamental questions about how scavenging or predation influence pathogen transmission. A key lens for our understanding of predator-prey and scavenging dynamics include behaviorally-mediated trade-offs weighed by consumers between nutritional gains and pathogen exposure risks. Consequently, the degree to which pathogens and associated diseases perpetuate through food webs can be partly attributed to behavioral responses of predators and scavengers, particularly their selection or avoidance of diseased prey and infected carcasses. Even so, examinations of avoidance or preference by predators and scavengers to diseased carrion are underrepresented. Here we identify areas for future research focused on behavioral immunity that could illuminate where, when, and how pathogen transmission reverberates through ecological communities. While directly attributing behavioral responses to pathogen exposure may be challenging, particularly for organisms with low susceptibility to spillover, identifying these responses though experimentation or observation help describe complex systems regarding infectious disease.
A polygenic risk score model for psoriasis based on the protein interactions of psoriasis susceptibility loci
Authors: Antonatos Charalabos , Koskeridis Fotios , Ralliou Christiana M. , Evangelou Evangelos , Grafanaki Katerina , Georgiou Sophia , Tsilidis Konstantinos K. , Tzoulaki Ioanna , Vasilopoulos Yiannis
Published: November 2024

Introduction

Polygenic Risk Scores (PRS) are an emerging tool for predicting an individual’s genetic risk to a complex trait. Several methods have been proposed to construct and calculate these scores. Here, we develop a biologically driven PRS using the UK BioBank cohort through validated protein interactions (PPI) and network construction for psoriasis, incorporating variants mapped to the interacting genes of 14 psoriasis susceptibility (PSORS) loci, as identified from previous genetic linkage studies.

Methods

We constructed the PPI network via the implementation of two major meta-databases of protein interactions, and identified variants mapped to the identified PSORS-interacting genes. We selected only European unrelated participants including individuals with psoriasis and randomly selected healthy controls using an at least 1:4 ratio to maximize statistical power. We next compared our PPI-PRS model to (i) clinical risk models and (ii) conventional PRS calculations through p-value thresholding.

Results

Our PPI-PRS model provides comparable results to both clinical risk models and conventional approaches, despite the incorporation of a limited number of variants which have not necessarily reached genome-wide significance (GWS). Exclusion of variants mapped to the HLA-C locus, an established risk locus for psoriasis resulted in highly similar associations compared to our primary model, indicating the contribution of the genetic variability mapped to non-GWS variants in PRS computations.

Discussion

Our findings support the implementation of biologically driven approaches in PRS calculations in psoriasis, highlighting their potential clinical utility in risk assessment and treatment management.