bluuebunny/crossref_metadata_2025 huggingface dataset:
Spanish vocabulary-bridging technology-enhanced instruction for young English language learners’ word learning
Authors: Leacox Lindsey , Jackson Carla Wood
Published: November 2012

This study examined preschool and kindergarten English language learners (ELLs) attending a migrant summer programme and their vocabulary word learning during both adult-read and technology-enhanced repeated readings. In a within-subject design, 24 ELLs (four to six years old) engaged in repeated readings in a control and a treatment condition. In the control condition, small groups of children listened to an adult-read storybook, reading in English with incidental vocabulary exposure. In the treatment condition, a technology-enhanced English shared reading with Spanish-bridging vocabulary instruction (TESB) was provided with adult mediation in an electronic book (e-book). TESB consisted of multiple vocabulary strategies including a preview of target vocabulary words and audio-recorded Spanish vocabulary definitions embedded throughout the e-book. Research suggests that even brief vocabulary interventions increase word learning (NICHHD, 2000), and accordingly, results have revealed that children make gains in both conditions through incidental exposure (Elley, 1989) and explicit vocabulary instruction (Biemiller and Boote, 2006). Significantly, more word learning gains were made in the TESB treatment condition than in the adult reading condition, as measured by researcher-developed tasks on English receptive knowledge and English naming performance. Significant pre- to post-test differences demonstrated modest growth. Educational implications are discussed, as even short interventions can lead to vocabulary gains using vocabulary strategies to support learning.
Aliskiren, at low doses, reduces the electrical remodeling in the heart of the TGR(mRen2)27 rat independently of blood pressure
Authors: De Mello Walmor , Rivera Marielis , Rabell Andres , Gerena Yamil
Published: November 2012

Methods:

The influence of chronic administration of low doses of aliskiren (5 mg/kg/day, i.p.) for a period of eight weeks on cardiac electrophysiological and structural remodeling was investigated in transgenic (TGR)(mRen-2)27 rats. Cardiac and plasma angiotensin II (Ang II) levels were determined by ELISA before and after administration of the drug. Moreover, histological, electrophysiological and echocardiographic studies were performed in controls and at the end of eight weeks of aliskiren administration.

Results:

1) The cardiac Ang II levels were significantly reduced while the plasma Ang II levels were not significantly decreased in rats treated with low doses of aliskiren; 2) echocardographic studies showed a decrease of left ventricle diameter (LVD), left ventricle posterior wall thickness (LVPW), left ventricle end diastolic volume (LVEDV) and increased ejection fraction (EF); 3) aliskiren improved the impulse propagation, increased the cardiac refractoriness and reduced the incidence of triggered activity; 4) perivascular and interstitial fibrosis were greatly reduced, which explains the increase in conduction velocity. All these effects of aliskiren were found independently of blood pressure, suggesting that the beneficial effect of aliskiren was related to an inhibition of the local cardiac renin angiotensin system; and 5) the effect of mechanical stretch on action potential duration, conduction velocity and spontaneous rhythmicity was changed by aliskiren, supporting the hypothesis presented here that the beneficial effect of the drug on cardiac remodeling is related to a decreased sensitivity of cardiac muscle to mechanical stress.
Screening for primary aldosteronism in a primary care unit
Authors: Volpe Cristina , Wahrenberg Hans , Hamberger Bertil , Thorén Marja
Published: November 2012

Objective:

Primary aldosteronism (PA) is a common cause of secondary hypertension but the reported prevalence varies. Few studies have been carried out in primary care. We investigated the prevalence of PA by screening with the aldosterone to renin ratio (ARR).

Design and methods:

Patients with hypertension were recruited from a primary care unit and investigated in a university hospital setting. Of 235 patients asked to participate 77% accepted. Antihypertensive medication apart from amiloride and spironolactone was maintained. The cut-off level for a positive ARR was lower than in clinical practice (> 50 pmol/ng with aldosterone > 350 pmol/l) to adjust for any suppressive effects of medication. A positive ARR was followed by a confirmatory evaluation.

Results:

The frequency of confirmed PA was 1.6% and including cases with a positive ARR who refused further investigation it would be 3.3% at most. In primary hypertension angiotensin receptor blockers, angiotensin-converting enzyme inhibitors and thiazide diuretics markedly suppressed the ARR by increasing renin levels.

Conclusion:

The detection rate of PA in our study is in the lower part of the wide range shown in previous studies. Larger population studies are needed to establish the true prevalence in primary care. Future studies may clarify if the present cut-off levels allow detection of very mild PA.
Reclaiming two concepts of liberty
Authors: Elford Gideon
Published: November 2012

The article responds to an influential critique of the view that there is a conceptual distinction between kinds of liberty. The critique in question began with Gerald MacCallum Jr’s famous argument that liberty is a single concept that has a triadic structure between agent, constraint, and end. Against this view, the article argues that the triadic structure offered by MacCallum is unable to conceptualize a particular distinct understanding of liberty. Following Charles Taylor, the article defends the view that there is a distinct ‘exercise-concept’ of liberty that the triadic structure cannot account for. In support of this claim, the article contests a recent argument that an exercise-concept of liberty can be conceptualized in terms of the triadic structure. The article argues that the triadic framework can only conceptualize an exercise-concept of liberty at the cost of abandoning the substance of that framework. To defend this, the article explains that the triadic structure and an exercise-concept are basically conceptually distinct in virtue of the fact that the former embodies a ‘static’ concept, whereas the latter embodies a ‘dynamic’ one. To complete the argument that an exercise-concept is a distinct concept of liberty, the article articulates a common theme that unifies both the triadic structure and an exercise-concept as concepts of liberty.
The myth of isolates: ecosystem ecologies in the nuclear Pacific
Authors: DeLoughrey Elizabeth M.
Published: November 2012

This article explores how the concept of ecosystem ecologies, one of the most influential models of systems thinking, was developed in relation to the radioactive aftermath of US nuclear weapons testing in the Pacific Islands. Historian Richard Grove has demonstrated how tropical island colonies all over the globe served as vital laboratories and spaces of social, botanical, and industrial experiment in ways that informed modernity and the conservation movement. I propose a similar relationship between the militarized American island colonies of Micronesia and how their constitution as AEC laboratories contributed to both atomic modernity and the field of ecosystem ecology. This was enacted through metaphorical concepts of island isolation and distributed visually by Atomic Energy Commission films that upheld an aerial vision of the newly acquired atolls for an American audience. Finally, the myth of isolation is also at work in the ways in which Marshall Islanders exposed to nuclear fallout became human subjects for radiation experiments due to the idea of the biological isolate.
Sismonde de Sismondi's aristocratic republicanism
Authors: Urbinati Nadia
Published: November 2012

This article shows through Sismonde de Sismondi’s work how peculiarly modern issues like the revolution, equal political rights (universal suffrage) and an industrial and commercial society contributed to renewing the identity of republicanism. That renewal took place in Europe, after the French Revolution, and in a direct confrontation with democracy rather than liberalism. The problem in relation to which Sismondi reflected on the institutions of political liberty, the republican constitution and the role of individual liberty was the unstoppable growth of equality, political and social. In his work, republicanism was intended as an answer against the emergent democratic transformation of society, with a role of moderating equality, dividing and bridling popular sovereignty and containing the immoderate propensities of democracy. The modern threat to liberty did not originate from the power of the few or of the one, but from that of the many. It originated in the majoritarian principle on which democracy relies necessarily. This principle, the republican Sismondi argued, contains the seed of the suppression of minorities, that is to say of individual liberty.
Evolving on purpose: Results of a qualitative study to explore how public youth system reform advocates apply anti-oppressive practice frameworks in a collaborative training and action process
Authors: Nissen Laura Burney , Curry-Stevens Ann
Published: November 2012

The anti-oppressive practice (AOP) framework focuses on structural inequity and power relations, employing liberatory methods toward social justice goals. This article reports on a national training initiative in AOP and an associated participatory action research project to determine the transformative impact of the training on staff, services and the system itself. The setting of this study is among service providers in youth justice and treatment services, where racial disparities are pronounced. The project involved both skills development and application of AOP in ways that would promote systems reform. Participants found the experience to be valuable, relevant and energizing, and skill sets in AOP were developed. The course requirement to develop site-based projects in participants’ local settings created changes in the ways services were delivered. Participants valued the creation of ‘safe spaces’ to discuss their experiences of racism and oppression in the various youth service systems. Transformative learning theory is utilized as a backdrop to the experience of interpreting participant reactions and learning experiences. Results suggest that AOP is a useful framework for organizing renewed efforts to reduce disparities in justice and other service delivery systems. Participant projects, an overview of implications for the field, and suggested future research are discussed.
Effects of elevated perfusion pressure and pulsatile flow on human saphenous veins isolated from diabetic and non-diabetic patients
Authors: Rosique Marina JF , Rosique Rodrigo G , Tirapelli Luis F , Joviliano Edwaldo E , Dalio Marcelo B , Bassetto Solange , Rodrigues Alfredo J , Evora Paulo RB
Published: November 2012

Objective:

This study was carried out to determine high pressure and pulsatile flow perfusion effects on human saphenous vein (HSV) segments obtained from diabetic and non-diabetic patients.

Methods:

The veins were perfused with oxygenated Krebs solution for 3 h, with a pulsatile flow rate of 100 mL/min and pressures of 250 × 200 or 300 × 250 mmHg. After perfusion, veins were studied by light microscopy; nitric oxide synthase (NOS) isoforms, CD34 and nitrotyrosine immunohistochemistry and tissue nitrite/nitrate (NOx) and malondialdehyde (MDA) quantification.

Results:

Light microscopy revealed endothelial denuding areas in all HSV segments subjected to 300 × 250 mmHg perfusion pressure, but the luminal area was similar. The percentage of luminal perimeter covered by endothelium decreased as perfusion pressures increased, and significant differences were observed between groups. The endothelial nitric oxide synthase (eNOS) isoform immunostaining decreased significantly in diabetic patients’ veins independent of the perfusion pressure levels. The inducible NOS (iNOS), neuronal NOS (nNOS) and nitrotyrosine immunostaining were similar. Significant CD34 differences were observed between the diabetic 300 × 250 mmHg perfusion pressure group and the non-diabetic control group. Tissue nitrite/nitrate and MDA were not different among groups.

Conclusions:

Pulsatile flow and elevated pressures for 3 h caused morphological changes and decreased the eNOS expression in the diabetic patients’ veins.
Attitudes Supportive of Sexual Offending Predict Recidivism
Authors: Helmus Leslie , Hanson R. Karl , Babchishin Kelly M. , Mann Ruth E.
Published: November 2012

Attitudes supportive of sexual offending figure prominently in theories of sexual offending, as well as in contemporary assessment and treatment practices with sex offenders. Based on 46 samples ( n = 13,782), this meta-analysis found that attitudes supportive of sexual offending had a small, yet reasonably consistent, relationship with sexual recidivism (Cohen’s d = .22). To the extent that differences were observed, attitudes predicted recidivism better for child molesters than for rapists. There was no difference in the predictive accuracy of attitudes assessed at pretreatment or at posttreatment. The current study indicates that attitudes supportive of sexual offending is a psychologically meaningful risk factor for sex offenders. However, given that many different constructs have been designated as sex offender attitudes, further research and theory is needed to understand how these various constructs contribute to recidivism.
Testing the Validity of Gender Ideology Items by Implementing Probing Questions in Web Surveys
Authors: Behr Dorothée , Braun Michael , Kaczmirek Lars , Bandilla Wolfgang
Published: November 2012

This article examines the use of probing techniques in web surveys to identify validity problems of items. Conventional cognitive interviewing is usually based on small sample sizes and thus precludes quantifying the findings in a meaningful way or testing small or special subpopulations characterized by their response behavior. This article investigates probing in web surveys as a supplementary way to look at item validity. Data come from a web survey in which respondents were asked to give reasons for selecting a response category for a closed question. The web study was conducted in Germany, with respondents drawn from online panels ( n = 1,023). The usefulness of the proposed approach is shown by revealing validity problems with a gender ideology item.
Regathering the Imagined Audience
Authors: Stankiewicz Damien
Published: November 2012

Based on fifteen months of fieldwork with television and new media producers at the transnational “European” public television channel, ARTE, this article describes the ways in which staff are in the midst of reimagining the channel’s “audience.” On the one hand, staff continue to understand television audiences as primarily national, and only nationally coherent; on the other hand, ARTE’s audiences are understood to be fast-dispersing as a result of new broadcast technologies and streaming web content. To reach dispersing audiences, and to better articulate its programming across borders, ARTE has partly shifted its efforts toward regathering its public through international events, festivals, and other off-screen engagements where its viewers become differently coherent and knowable. The article argues that such a strategy re-emplaces “fans and followers” of ARTE in ways that might foster collective identifications in ways that may be literally replacing those engendered by traditional public broadcasting.
Memory and the West
Authors: Aoki Eric , Dickinson Greg , Ott Brian L.
Published: November 2012

The research collected in this special issue demonstrates that memory is mobilized symbolically and materially, spatially and temporally. It is fully embodied and often works affectively.
Clock Drawing Test in Elderly Individuals With Different Education Levels
Authors: Cecato Juliana Francisca , Fiorese Bruna , Montiel José Maria , Bartholomeu Daniel , Martinelli José Eduardo
Published: November 2012

Objective:

The aim of this study was to describe the performance in Clock Drawing Test (CDT) of the elderly individuals assessed in a geriatric clinic, with at least 1 year of schooling, comparing with other groups with higher education and with  Clinical Dementia Rating (CDR) levels. The study also aims to correlate the results of CDT and other used diagnostic tests for dementia by CDR levels, providing additional validity evidence to the CDT.

Methods:

Cross-sectional study with 426 elderly individuals, >60 years old and at least 1 year of education. All participants searched for medical assistance at Geriatric and Gerontology Ambulatory of Jundiaí city, in Brazil. The community-dwelling outpatients previously undergone a detailed clinical examination and neuropsychological evaluation: Cambrigde Cognitive Examination (CAMCOG), Mini-Mental State Examination (MMSE), andCDT. To differentiate data from diagnostic groups based on CDR, it Kruskal-Wallis test was used. Pearson statistics were calculated to compare data from CDT and CDR. The statistical analyses were 2-tailed and were considered significant when P < .05.

Results:

Regarding CDT, groups with more years of schooling showed similar means in CDR = 0 and CDR = 0.5 and in CDR = 1 and CDR = 2. Shulman and Sunderland scale were high score in groups with more years of education and above of cutoff points in all CDT score. On the contrary, in Mendez scale we did not observed similar means. Otherwise, in the group with less years of schooling greater means differences in the CDT were observed.

Conclusion:

The CDT did not show a strong correlation with MMSE and CAMCOG, both important instruments in Brazilian population to investigate dementia. For elderly individuals with high education levels, the CDT did not seem to be a good test to detect cognitive impairment.
On and Out of Revolution: Between public law and religion
Authors: Diamantides Marinos
Published: November 2012

While advocates and critics of liberal republics disagree on whether “pure politics” requires ultimate authorization both call upon theories that explain all revolutions as attempts to transcend political theology for the sake of a purely immanent political realm. Their secularist, “political”/programmatic views and hopes on revolution are here contrasted with a reading of Eugene Rosenstock-Huessy’s Out of Revolution whereby “revolution” is read as the autobiography of western man written through a series of great European Revolutions. As products of changes in the vocabulary of occidental Christian political theology these revolutions were structurally unable to transcend authority; but they were able to project western power around the world. Lacking in awareness of its religious vocabulary the late-modern subject inherits this global western power but can no longer rebel as before. The article summarizes Rosenstock-Huessy’s genealogy of revolution in Section II; in Sections I and 3 his insights are brought to bear on theories of revolution-qua-secularization be it in the form of the utopian overcoming of “religion” (Arendt) or in the form of overcoming Christianity’s fabulous and real history – its political theology – while retaining its universality for the sake of emancipation of a “universal” – not western – victim.
Priapism associated with risperidone: a case report, literature review and review of the South London and Maudsley hospital patients’ database
Authors: Paklet Lise , Abe Anne Mary , Olajide Dele
Published: November 2012

Priapism is a urological emergency defined as persistent penile erection that is unrelated to sexual stimulation and typically involving only the corporal cavernosa. It can occur as a rare side effect of antipsychotic medications and is mediated via their α-adrenergic antagonist effect. In this paper we describe a case of priapism in a patient started on risperidone and sodium valproate. We also review the South London and Maudsley Case Register Interactive Search database to assess how many other cases of priapism were reported in patients taking risperidone. We add this information to a literature review of cases of priapism associated with risperidone.
A Review of Periprosthetic Femoral Fractures Associated With Total Hip Arthroplasty
Authors: Marsland Daniel , Mears Simon C.
Published: November 2012

Periprosthetic fractures of the femur in association with total hip arthroplasty are increasingly common and often difficult to treat. Patients with periprosthetic fractures are typically elderly and frail and have osteoporosis. No clear consensus exists regarding the optimal management strategy because there is limited high-quality research. The Vancouver classification facilitates treatment decisions. In the presence of a stable prosthesis (type-B1 and -C fractures), most authors recommend surgical stabilization of the fracture with plates, strut grafts, or a combination thereof. In up to 20% of apparent Vancouver type-B1 fractures, the femoral stem is loose, which may explain the high failure rates associated with open reduction and internal fixation. Some authors recommend routine opening and dislocation of the hip to perform an intraoperative stem stability test to rule out a loose component. Advances in plating techniques and technology are improving the outcomes for these fractures. For fractures around a loose femoral prosthesis (types B2 and 3), revision using an extensively porous-coated uncemented long stem, with or without additional fracture fixation, appears to offer the most reliable outcome. Cement-in-cement revision using a long-stem prosthesis is feasible in elderly patients with a well-fixed cement mantle. It is essential to treat the osteoporosis to help fracture healing and to prevent further fractures. We provide an overview of the causes, classification, and management of periprosthetic femoral fractures around a total hip arthroplasty based on the current best available evidence.
The Impact of Comorbidity on Perioperative Outcomes of Hip Fractures in a Geriatric Fracture Model
Authors: Menzies Isaura B. , Mendelson Daniel A. , Kates Stephen L. , Friedman Susan M.
Published: November 2012

Background:

Older adults who sustain hip fractures usually have multiple coexisting medical problems that may impact their treatment and outcomes. The geriatric fracture center (GFC) provides a model of care that standardizes treatment and optimizes outcomes. The purpose of this study is to determine whether GFC patients with a higher burden of comorbidity or specific comorbidities are at risk for worsened perioperative outcomes, such as increased time to surgery (TTS), postoperative complications, and longer length of hospital stay (LOS).

Method:

A total of 1077 patients aged 60 years and older who underwent surgery for a proximal femur fracture between April 15, 2005, and September 30, 2010, were evaluated. Comorbidities measured in the Charlson Comorbidity index were abstracted through chart review. Outcomes were TTS, postoperative complications, and LOS.

Results:

Most patients were white, with an average age of 85. One half lived in either a nursing home or an assisted living facility. The mean Charlson score was 3.06 and the nursing home residents had a significantly higher score compared to community dwellers (3.4 vs 2.8; P < .0001). Dementia was the most common comorbidity. There was no difference in the LOS or TTS based on Charlson score. The overall complication rate was 44% with delirium being the most common postoperative complication. Peripheral vascular disease, history of solid tumor, and peptic ulcer disease predicted delirium incidence. Charlson score predicted complication risk, with an odds ratio of 1.12 for each point increase.

Conclusion:

Frailty and comorbidity put this hip fracture population at high risk for adverse perioperative outcomes. This study shows that in the GFC model of care the comorbidity burden did not impact the TTS and LOS but did predict postoperative complication rate.
Sequencing of the needle transcriptome from Norway spruce (Picea abies Karst L.) reveals lower substitution rates, but similar selective constraints in gymnosperms and angiosperms
Authors: Chen Jun , Uebbing Severin , Gyllenstrand Niclas , Lagercrantz Ulf , Lascoux Martin , Källman Thomas
Published: November 2012

Background

A detailed knowledge about spatial and temporal gene expression is important for understanding both the function of genes and their evolution. For the vast majority of species, transcriptomes are still largely uncharacterized and even in those where substantial information is available it is often in the form of partially sequenced transcriptomes. With the development of next generation sequencing, a single experiment can now simultaneously identify the transcribed part of a species genome and estimate levels of gene expression.

Results

mRNA from actively growing needles of Norway spruce (Picea abies) was sequenced using next generation sequencing technology. In total, close to 70 million fragments with a length of 76 bp were sequenced resulting in 5 Gbp of raw data. A de novo assembly of these reads, together with publicly available expressed sequence tag (EST) data from Norway spruce, was used to create a reference transcriptome. Of the 38,419 PUTs (putative unique transcripts) longer than 150 bp in this reference assembly, 83.5% show similarity to ESTs from other spruce species and of the remaining PUTs, 3,704 show similarity to protein sequences from other plant species, leaving 4,167 PUTs with limited similarity to currently available plant proteins. By predicting coding frames and comparing not only the Norway spruce PUTs, but also PUTs from the close relatives Picea glauca and Picea sitchensis to both Pinus taeda and Taxus mairei, we obtained estimates of synonymous and non-synonymous divergence among conifer species. In addition, we detected close to 15,000 SNPs of high quality and estimated gene expression differences between samples collected under dark and light conditions.

Conclusions

Our study yielded a large number of single nucleotide polymorphisms as well as estimates of gene expression on transcriptome scale. In agreement with a recent study we find that the synonymous substitution rate per year (0.6 × 10−09and 1.1 × 10−09) is an order of magnitude smaller than values reported for angiosperm herbs. However, if one takes generation time into account, most of this difference disappears. The estimates of the dN/dS ratio (non-synonymous over synonymous divergence) reported here are in general much lower than 1 and only a few genes showed a ratio larger than 1.
Evolution and diversity of periplasmic proteins involved in copper homeostasis in gamma proteobacteria
Authors: Hernández-Montes Georgina , Argüello José M , Valderrama Brenda
Published: November 2012

Background

Different systems contributing to copper homeostasis in bacteria have been described in recent years involving periplasmic and transport proteins that provide resistance via metal efflux to the extracellular media (CopA/Cue, Cus, Cut, and Pco). The participation of these proteins in the assembly of membrane, periplasmic and secreted cuproproteins has also been postulated. The integration and interrelation of these systems and their apparent redundancies are less clear since they have been studied in alternative systems. Based on the idea that cellular copper is not free but rather it is transferred via protein-protein interactions, we hypothesized that systems would coevolve and be constituted by set numbers of essential components.

Results

By the use of a phylogenomic approach we identified the distribution of 14 proteins previously characterized as members of homeostasis systems in the genomes of 268 gamma proteobacteria. Only 3% of the genomes presented the complete systems and 5% of them, all intracellular parasites, lacked the 14 genes. Surprisingly, copper homeostatic pathways did not behave as evolutionary units with particular species assembling different combinations of basic functions. The most frequent functions, and probably because of its distribution the most vital, were copper extrusion from the cytoplasm to the periplasm performed by CopA and copper export from the cytoplasm to the extracellular space performed by CusC, which along with the remaining 12 proteins, assemble in nine different functional repertoires.

Conclusions

These observations suggest complex evolutionary dynamics and still unexplored interactions to achieve copper homeostasis, challenging some of the molecular transport mechanism proposed for these systems.
Spontaneous dissection of the coronary and vertebral arteries post-partum: case report and review of the literature
Authors: Cenkowski Marta , daSilva Megan , Bordun Kimberly-Ann , Hussain Farrukh , Kirkpatrick Iain DC , Jassal Davinder S
Published: November 2012

Background

Spontaneous coronary and vertebral artery dissections are rare events occurring most commonly in otherwise healthy women during pregnancy or the post-partum period.

Case presentation

This report describes a 35-year-old female who presented with an acute inferior ST elevation myocardial infarction 7 months post-partum secondary to spontaneous dissection of the left obtuse marginal coronary artery. Despite appropriate medical therapy with dual anti-platelet therapy, the patient presented four weeks later with a spontaneous dissection of the right vertebral artery.

Conclusion

We review the presentation, diagnosis, and management of spontaneous dissections of the vasculature in the peri-partum period.
Men’s and women’s exposure and perpetration of partner violence: an epidemiological study from Sweden
Authors: Lövestad Solveig , Krantz Gunilla
Published: November 2012

Background

Over the past 30 years, intimate partner violence (IPV) against women and its health consequences has become a well established research area and is recognized worldwide as a significant public health issue. Studies on IPV directed at men are less explored, however recently women’s use of IPV and men’s victimization is gaining growing attention. Earlier population-based studies performed in Sweden have primarily investigated men’s violence against women, while women’s use of violence and men’s exposure as well as the existence of controlling behaviours have been neglected research areas This explorative study investigated the exposure to and perpetration of intimate partner violence, the use of control behaviours and the associated risk factors among a sample of Swedish men and women.

Methods

This cross-sectional population-based study included 173 men and 251 women of age 18–65 randomly selected among the Swedish population. A questionnaire based on the revised Conflicts Tactics Scale (CTS2) and the subscale ‘isolating control’ from the Controlling Behaviour Scale (CBS) was used to collect data on violence exposure and perpetration. Regression analyses were used for risk factor assessment.

Results

More men (11%) than women (8%) reported exposure to physical assault in the past year, while more women reported exposure to sexual coercion. Duration of present relationship ≤ 3 years was identified as a significant risk factor for men’s exposure. Young age, lack of social support and being single, constituted risk factors for women’s exposure. Surprisingly many men (37%) and women (41%) also reported exposure to controlling behaviours.

Conclusions

In partner violence research, both men’s and women’s exposure should be explored however findings need to be interpreted with caution. This first study in a Swedish sample establishes the basis for future investigations on partner violence and coercive control tactics.
Health-related quality of life and needs of care and support of adult Tanzanians with cancer: a mixed-methods study
Authors: Masika Golden M , Wettergren Lena , Kohi Thecla W , von Essen Louise
Published: November 2012

Background

Cancer is among the three leading causes of death in low income countries and the highest increase with regard to incidence figures for cancer diseases are found in these countries. This is the first report of the health-related quality of life (HRQOL) and needs of care and support of adult Tanzanians with cancer.

Methods

A mixed-methods design was used. The study was conducted at Ocean Road Cancer Institute (ORCI) in Dar es Salaam, Tanzania. One hundred and one patients with a variety of cancer diagnoses treated and cared for at ORCI answered the Kiswahili version of the EORTC QLQ-C30 investigating HRQOL. Thirty-two of the patients participated in focus group interviews discussing needs of care and support. Data from focus group interviews were analyzed with content analysis.

Results

The findings show that the patients, both women and men, report a low quality of life, especially with regard to physical, role, and social function and a high level of symptoms and problems especially with financial difficulties and pain. Financial difficulties are reported to a remarkably high extent by both women and men. The patients, both women and men report least problems with emotional function. A content analysis of the interview data revealed needs of food and water, hygienic needs, emotional needs, spiritual needs, financial needs, and needs of closeness to cancer care and treatment services.

Conclusion

The high score for pain points out that ORCI is facing severe challenges regarding care and treatment. However, when considering this finding it should be noted that the pain subscale of the Kiswahili version of the EORTC QLQ-C30 did not reach acceptable internal consistency and showed less than satisfactory convergent validity. This also applies to the subscales cognitive function and global health/quality of life. Attention should be drawn to meet the identified needs of Tanzanian cancer patients while hospitalized but also when at home. Increased accessibility of mosquito nets, pads, and pain-killers would help to fulfil some needs.
Primary bladder adenocarcinoma versus metastatic colorectal adenocarcinoma: a persisting diagnostic challenge
Authors: Roy Somak , Smith Matthew A , Cieply Kathy M , Acquafondata Marie B , Parwani Anil V
Published: November 2012

Aim

This study attempted to distinguish primary bladder adenocarcinoma (PBA) from metastatic colonic adenocarcinomas (MCA), which is a difficult diagnostic and clinical problem.

Methods

Twenty-four cases of bladder adenocarcinomas (12 primary & 12 metastatic colorectal) were included in the study with urothelial carcinoma (UC) and colonic adenocarcinoma (CA) as controls. A panel of immunohistochemical (IHC) stains along with fluorescence in-situ hybridization (FISH), using the UroVysion probe set, was performed.

Results

The majority of the PBAs presented with advanced disease. Enteric histologic subtype was the most common morphological variant. Strong nuclear with cytoplasmic-membranous staining of β-catenin was seen in 75% of MCA and only 16.7% PBA (<10% staining cells). Although abnormal nuclear staining with E-cadherin was seen in both PBA and MCA, it was more frequent in former. CK-7, CK-20, villin and CDX-2 stains were not helpful in distinguishing the two entities. FISH did not reveal any unique differences in chromosomal abnormality between the two groups.

Conclusion

Although there was a statistically significant difference in β-catenin and E-cadherin staining between two groups, we did not find any IHC or FISH marker that was specific for PBA. Distinction between PBA and MCA remains a diagnostic problem and clinical correlation is vital before rendering a diagnosis.

Virtual slides

The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1393156268152357
Gamma-H2AX foci in cells exposed to a mixed beam of X-rays and alpha particles
Authors: Staaf Elina , Brehwens Karl , Haghdoost Siamak , Czub Joanna , Wojcik Andrzej
Published: November 2012

Background

Little is known about the cellular effects of exposure to mixed beams of high and low linear energy transfer radiation. So far, the effects of combined exposures have mainly been assessed with clonogenic survival or cytogenetic methods, and the results are contradictory. The gamma-H2AX assay has up to now not been applied in this context, and it is a promising tool for investigating the early cellular response to mixed beam irradiation.

Purpose

To determine the dose response and repair kinetics of gamma-H2AX ionizing radiation-induced foci in VH10 human fibroblasts exposed to mixed beams of 241Am alpha particles and X-rays.

Results

VH10 human fibroblasts were irradiated with each radiation type individually or both in combination at 37°C. Foci were scored for repair kinetics 0.5, 1, 3 and 24 h after irradiation (one dose per irradiation type), and for dose response at the 1 h time point. The dose response effect of mixed beam was additive, and the relative biological effectiveness for alpha particles (as compared to X-rays) was of 0.76 ± 0.52 for the total number of foci, and 2.54 ± 1.11 for large foci. The repair kinetics for total number of foci in cells exposed to mixed beam irradiation was intermediate to that of cells exposed to alpha particles and X-rays. However, for mixed beam-irradiated cells the frequency and area of large foci were initially lower than predicted and increased during the first 3 hours of repair (while the predicted number and area did not).

Conclusions

The repair kinetics of large foci after mixed beam exposure was significantly different from predicted based on the effect of the single dose components. The formation of large foci was delayed and they did not reach their maximum area until 1 h after irradiation. We hypothesize that the presence of low X-ray-induced damage engages the DNA repair machinery leading to a delayed DNA damage response to the more complex DNA damage induced by alpha particles.
Short-Term Pharmacological Suppression of the Hyperprolactinemia of Infertile hCG-Overproducing Female Mice Persistently Restores Their Fertility
Authors: Ratner Laura D. , Gonzalez Betina , Ahtiainen Petteri , Di Giorgio Noelia P. , Poutanen Matti , Calandra Ricardo S. , Huhtaniemi Ilpo T. , Rulli Susana B.
Published: November 2012

Female infertility is often associated with deregulation of hormonal networks, and hyperprolactinemia is one of the most common endocrine disorders of the hypothalamic-pituitary axis affecting the reproductive functions. We have shown previously that transgenic female mice overexpressing human chorionic gonadotropin β-subunit (hCGβ+ mice), and producing elevated levels of bioactive LH/hCG, exhibit increased production of testosterone and progesterone, are overweight and infertile, and develop hyperprolactinemia associated with pituitary lactotrope adenomas in adult age. In the present study, we analyzed the influence of the hyperprolactinemia of hCGβ+ females on their reproductive phenotype by treating them with the dopamine agonists, bromocriptine and cabergoline. Long-term bromocriptine treatment of adult mice was effective in the control of obesity, pituitary growth, and disturbances in the hormone profile, demonstrating that hyperprolactinemia was the main cause of the hCGβ+ female phenotype. Interestingly, short-term treatment (1 wk) with cabergoline applied on 5-wk-old mice corrected hyperprolactinemia, hyperandrogenism, and hyperprogesteronemia, prevented pituitary overgrowth, normalized gonadal function, and recovered fertility of adult hCGβ+ females after hormone-induced and natural ovulation. The same cabergoline treatment in the short term applied on 3-month-old hCGβ+ females failed to recover their reproductive function. Hence, we demonstrated that the short-term cabergoline treatment applied at a critical early stage of the phenotype progression effectively prevented the hyperprolactinemia-associated reproductive dysfunction of hCG-overproducing females.
Autophagy Activity Is Up-Regulated in Adipose Tissue of Obese Individuals and Modulates Proinflammatory Cytokine Expression
Authors: Jansen H. J. , van Essen P. , Koenen T. , Joosten L. A. B. , Netea M. G. , Tack C. J. , Stienstra R.
Published: November 2012

Autophagy, an evolutionary conserved process aimed at recycling damaged organelles and protein aggregates in the cell, also modulates proinflammatory cytokine production in peripheral blood mononuclear cells. Because adipose tissue inflammation accompanied by elevated levels of proinflammatory cytokines is characteristic for the development of obesity, we hypothesized that modulation of autophagy alters adipose tissue inflammatory gene expression and secretion. We tested our hypothesis using ex vivo and in vivo studies of human and mouse adipose tissue. Levels of the autophagy marker LC3 were elevated in sc adipose tissue of obese vs. lean human subjects and positively correlated to both systemic insulin resistance and morphological characteristics of adipose tissue inflammation. Similarly, autophagic activity levels were increased in adipose tissue of obese and insulin resistant animals as compared with lean mice. Inhibition of autophagy by 3-methylalanine in human and mouse adipose tissue explants led to a significant increase in IL-1β, IL-6, and IL-8 mRNA expression and protein secretion. Noticeably, the enhancement in IL-1β, IL-6, and keratinocyte-derived chemoattractant (KC) by inhibition of autophagy was more robust in the presence of obesity. Similar results were obtained by blocking autophagy using small interfering RNA targeted to ATG7 in human Simpson-Golabi-Behmel syndrome adipocytes. Our results demonstrate that autophagy activity is up-regulated in the adipose tissue of obese individuals and inhibition of autophagy enhances proinflammatory gene expression both in adipocytes and adipose tissue explants. Autophagy may function to dampen inflammatory gene expression and thereby limit excessive inflammation in adipose tissue during obesity.
Activin Signaling Regulates Sertoli Cell Differentiation and Function
Authors: Nicholls Peter K. , Stanton Peter G. , Chen Justin L. , Olcorn Justine S. , Haverfield Jenna T. , Qian Hongwei , Walton Kelly L. , Gregorevic Paul , Harrison Craig A.
Published: November 2012

Throughout development, activin A signaling stimulates proliferation and inhibits differentiation of testicular Sertoli cells. A decline in activin levels at puberty corresponds with the differentiation of Sertoli cells that is required to sustain spermatogenesis. In this study, we consider whether terminally differentiated Sertoli cells can revert to a functionally immature phenotype in response to activin A. To increase systemic activin levels, the right tibialis anterior muscle of 7-wk-old C57BL/6J mice was transduced with an adeno-associated virus (rAAV6) expressing activin A. We show that chronic activin signaling reduces testis mass by 23.5% compared with control animals and induces a hypospermatogenic phenotype, consistent with a failure of Sertoli cells to support spermatogenesis. We use permeability tracers and transepithelial electrical resistance measurements to demonstrate that activin potently disrupts blood-testis-barrier function in adult mice and ablates tight junction formation in differentiated primary Sertoli cells, respectively. Furthermore, increased activin signaling reinitiates a program of cellular proliferation in primary Sertoli cells as determined by 5-ethynyl-2′-deoxyuridine incorporation. Proliferative cells reexpress juvenile markers, including cytokeratin-18, and suppress mature markers, including claudin-11. Thus, activin A is the first identified factor capable of reprogramming Sertoli cells to an immature, dedifferentiated phenotype. This study indicates that activin signaling must be strictly controlled in the adult in order to maintain Sertoli cell function in spermatogenesis.
The Role of Adiponectin in Cancer: A Review of Current Evidence
Authors: Dalamaga Maria , Diakopoulos Kalliope N. , Mantzoros Christos S.
Published: November 2012

Excess body weight is associated not only with an increased risk of type 2 diabetes and cardiovascular disease (CVD) but also with various types of malignancies. Adiponectin, the most abundant protein secreted by adipose tissue, exhibits insulin-sensitizing, antiinflammatory, antiatherogenic, proapoptotic, and antiproliferative properties. Circulating adiponectin levels, which are determined predominantly by genetic factors, diet, physical activity, and abdominal adiposity, are decreased in patients with diabetes, CVD, and several obesity-associated cancers. Also, adiponectin levels are inversely associated with the risk of developing diabetes, CVD, and several malignancies later in life. Many cancer cell lines express adiponectin receptors, and adiponectin in vitro limits cell proliferation and induces apoptosis. Recent in vitro studies demonstrate the antiangiogenic and tumor growth-limiting properties of adiponectin. Studies in both animals and humans have investigated adiponectin and adiponectin receptor regulation and expression in several cancers. Current evidence supports a role of adiponectin as a novel risk factor and potential diagnostic and prognostic biomarker in cancer. In addition, either adiponectin per se or medications that increase adiponectin levels or up-regulate signaling pathways downstream of adiponectin may prove to be useful anticancer agents.

This review presents the role of adiponectin in carcinogenesis and cancer progression and examines the pathophysiological mechanisms that underlie the association between adiponectin and malignancy in the context of a dysfunctional adipose tissue in obesity. Understanding of these mechanisms may be important for the development of preventive and therapeutic strategies against obesity-associated malignancies.
Molecular Mechanisms of Prolactin and Its Receptor
Authors: Brooks Charles L.
Published: November 2012

Prolactin and the prolactin receptors are members of a family of hormone/receptor pairs which include GH, erythropoietin, and other ligand/receptor pairs. The mechanisms of these ligand/receptor pairs have broad similarities, including general structures, ligand/receptor stoichiometries, and activation of several common signaling pathways. But significant variations in the structural and mechanistic details are present among these hormones and their type 1 receptors. The prolactin receptor is particularly interesting because it can be activated by three sequence-diverse human hormones: prolactin, GH, and placental lactogen. This system offers a unique opportunity to compare the detailed molecular mechanisms of these related hormone/receptor pairs. This review critically evaluates selected literature that informs these mechanisms, compares the mechanisms of the three lactogenic hormones, compares the mechanism with those of other class 1 ligand/receptor pairs, and identifies information that will be required to resolve mechanistic ambiguities. The literature describes distinct mechanistic differences between the three lactogenic hormones and their interaction with the prolactin receptor and describes more significant differences between the mechanisms by which other related ligands interact with and activate their receptors.