bluuebunny/crossref_metadata_2025 huggingface dataset:
Changes in the Use of Comprehensive Geriatric Assessment in Clinical Trials for Older Patients with Cancer over Time
Authors: Le Saux Olivia , Falandry Claire , Gan Hui K. , You Benoit , Freyer Gilles , Péron Julien
Published: February 2019

Background

The objective of this study was to describe the implementation of comprehensive geriatric assessment (CGA) in clinical trials dedicated to older patients before and after the creation of the International Society of Geriatric Oncology in the early 2000s.

Subjects, Materials, and Methods

All phase I, II, and III trials dedicated to the treatment of cancer among older patients published between 2001 and 2004 and between 2011 and 2014 were reviewed. We considered that a CGA was performed when the authors indicated an intention to do so in the Methods section of the article. We collected each geriatric domain assessed using a validated tool even in the absence of a clear CGA, including nutritional, functional, cognitive, and psychological status, comorbidity, comedication, overmedication, social status and support, and geriatric syndromes.

Results

A total of 260 clinical trials dedicated to older patients were identified over the two time periods: 27 phase I, 193 phase II, and 40 phase III trials. CGA was used in 9% and 8% of phase II and III trials, respectively; it was never used in phase I trials. Performance status was reported in 67%, 79%, and 75% of phase I, II, and III trials, respectively. Functional assessment was reported in 4%, 11%, and 13% of phase I, II, and III trials, respectively. Between the two time periods, use of CGA increased from 1% to 11% (p = .0051) and assessment of functional status increased from 3% to 14% (p = .0094).

Conclusion

The use of CGA in trials dedicated to older patients increased significantly but remained insufficient.
Assessment of numerical procedures for determining shallow foundation failure envelopes
Authors: Suryasentana Stephen K. , Dunne Helen P. , Martin Christopher M. , Burd Harvey J. , Byrne Byron W. , Shonberg Avi
Published: February 2019

The failure envelope approach is commonly used to assess the capacity of shallow foundations under combined loading, but there is limited published work that compares the performance of various numerical procedures for determining failure envelopes. This paper addresses this issue by carrying out a detailed numerical study to evaluate the accuracy, computational efficiency and resolution of these numerical procedures. The procedures evaluated are the displacement probe test, the load probe test, the swipe test (referred to in this paper as the single swipe test) and a less widely used procedure called the sequential swipe test. Each procedure is used to determine failure envelopes for a circular surface foundation and a circular suction caisson foundation under planar vertical, horizontal and moment (VHM) loading for a linear elastic, perfectly plastic von Mises soil. The calculations use conventional, incremental-iterative finite-element analysis (FEA) except for the load probe tests, which are performed using finite-element limit analysis (FELA). The results demonstrate that the procedures are similarly accurate, except for the single swipe test, which gives a load path that under-predicts the failure envelope in many of the examples considered. For determining a complete VHM failure envelope, the FEA-based sequential swipe test is shown to be more efficient and to provide better resolution than the displacement probe test, while the FELA-based load probe test is found to offer a good balance of efficiency and accuracy.
DNA Extraction using FastDNA Spinkit for Soil with PolyA Modifictaion v1
Authors: D'angelo Tim
Published: February 2019

This protocol describes a modification to the MP Biomedicals FastDNA SpinKit for Soil. It has been changed to use a Retsch MM400 Mixer Mill instead of the MP Biomedicals bead beating machine. The addition of polyadenylic acid (poly(A)) is also used, which we have emperically determined increases DNA yeild from basalt substrates. This protocol is a modification from the protocol described in Jorgensen and Zhao (2016). Here we do not use the Amplicon G2 DNA/RNA Enhancer Tube because we found that it did not markedly increase DNA yeilds. See figure below. qPCR analysis from DNA extracts of two incubated Basalt samples. Results show that using the Amplicon G2 DNA/RNA Enhancer tube does not markedy increase DNA yeild on Basalt samples. FastDNA = Following the MP Biomedicals Protocol, FastDNA + PolyA = As described in this protocol, FastDNA + PolyA + G2 = As described in this protocol but using the G2 Tube instead of the MP Biomedicals bead tube. NOTE: polyA sometimes creates false-positive readings for no-template control extractions when measuring DNA concentration with the Qbit Fluorometric Assay. Quantitive-PCR will allow you to determine if NTC samples are actually contaminated. A description of a similar protocol can be found here: Jorgensen, SL. And Zhao, R. (2016).Microbial Inventory of Deeply Buried Oceanic Crust from a Young Ridge Flank. Front. Microbio. 7
Lake plankton sample collection from the field for downstream molecular analysis v1
Authors: Domaizon Isabelle , Kurmayer Rainer , Capelli Camilla , Chardon Cécile , Hufnagl Peter , Vautier Marine , Salmaso Nico
Published: February 2019

The objective of this protocol is to provide a reliable and replicable method for the sampling of lake micro-plankton to be used for downstream DNA analysis. This protocol is one of those proposed by the EcoAlpsWater consortium to promote the implementation of High Throughput Sequencing (HT S) of environmental DNA (eDNA) in the biomonitoring and ecological assessment of water bodies. The application proposed here, in the context of EcoAlpsWater, aims at comparing DNA inventories to traditional phytoplanktonic inventories and at characterizing more broadly the micro-planktonic diversity through eDNA analysis (including bacteria). The sampling strategy is similar to that used for classical phytoplankton survey focusing on the euphotic zone, however the procedure for filtration and preservation is adapted for DNA samples. This protocol is part of the deliverables provided by the WP1 of the Eco-AlpsWater project. All members of the EcoAlpsWater consortium (http://www.alpine-space.eu/projects/eco-alpswater/en/home) contributed to the optimization of this protocol.
BYOD with Security
Authors: Neves Ulysses Moreira , Mello Flávio Luis de
Published: February 2019

The concern of companies to keep sensitive data protected from improper access and information leaking has grown a lot. The constant cases of industrial espionage and information leakage regarding companies are an evidence of the need to apply strict information security policies, improve data protection and allow an auditing track. With the evolution of technology, the usage of personal mobile devices increased in organizations (BYOD - Bring Your Own Device), which allows the employees to use their own mobile devices at work. This paper addresses the current challenges faced by IT companies and teams in protecting access to this kind of information, and what strategies are used to mitigate, to track leaks, and reduce the misuse of documents in the organization. Considering the scenario evaluated, a framework with good Information Security practices based on the ISO 27002:2005 and the practical controls of the Center of Internet Security (CIS) is proposed, associating good practices with the needs of BYOD’s culture. The framework suggested in this paper reinforces the necessity for a standardization of the rules of information security in the process of adoption of BYOD’s culture, following the life cycle of the user with his personal mobile device in the company.

 

 
Click Fraud Detection and Prevention System for Ad Networks
Authors: Almeida Paulo S. , Gondim João J. C.
Published: February 2019

Click fraud detection consists of identifying the intention behind received clicks, given only technical data and context information. Reviewing concepts involved in click fraud practices and related work, a system that detects and prevents this type of fraud is proposed and implemented. The system is based and implemented on an ad network, one of the 3 main agents in the online ad environment, and for its validation, 3 servers were used, representing the publisher, the ad network with the system implemented and the announcer, and a bot that attempts to commit a click fraud.
Activity of nucleolar organizers in endometrial glandular and surface epithelial cells in patients with external genital endometriosis
Authors: Kurenkov Evgeny L. , Igenbaeva Elena V. , Uzlova Tatyana V.
Published: February 2019

Hypothesis/aims of study. Endometriosis is a disease characterized by proliferation of tissue that is similar in morphology and function to the endometrium, outside the uterine cavity. Thanks to the theory of J. Sampson (1927), which explains endometrioid heterotopia formation by bits of endometrial tissue migrating through the fallopian tubes during menstruation and their subsequent implantation in the pelvis, research has begun on the biological properties of eutopic endometrial cells in women with endometriosis, which is still relevant today. The present study was aimed to assess the activity of nucleolus organizers in the endometrium in women with external genital endometriosis and without it at different stages of the menstrual cycle.

Study design, materials, and methods. Paraffin blocks of endometrial biopsy specimens were taken from 68 women with and without external genital endometriosis. The activity of nucleolar organizers was evaluated by the method of J. Crocker (1983).

Results. The data obtained showed a significant increase in ribosomal activity in the cells of the endometrial glandular and surface epithelium in women with external genital endometriosis at the stages of late proliferation, as well as early and middle secretions. There was also a significant decrease in the activity of the nucleolar organizers of endometrial epithelial cells at the stage of late secretion in women with external genital endometriosis.

Conclusion. The obtained differences in regulation of ribosomal synthesis in the cells of the integument and glandular epithelium of the endometrium in women with external genital endometriosis, in our opinion, can provide an increase in the total amount of epitheliocytes of the endometrium, increase their adhesive and invasive abilities, and lengthen the life cycle of cells. This contributes to the formation of endometrioid heterotopies and the development of external genital endometriosis. Changes in the activity of nucleolar organizers in the endometrium in women with external genital endometriosis may be of clinical interest for the diagnosis of this pathology.
Possible genetically determined mechanisms of arterial hypertension in tobacco smoking
Authors: Maltseva N. V. , Smirnova A. Sh. , Rublevskaya A. C. , Bichan N. A.
Published: February 2019

Te increase in the prevalence of arterial hypertension (AH) in populations, ineﬀective treatment, the need for risk stratifcation, prevention, early diagnosis and successful treatment, actualize genomic studies to develop a personalized therapeutic approach to AH. Te review investigates the possible genetically determined mechanisms of the development of hypertension and endothelial dysfunction caused by polymorphism of the genes of endothelial nitric oxide synthase (eNOS) and enzymes of phases I and II of the xenobiotics detoxifcation system. Te probable interaction of both systems under the inﬂuence of harmful environmental factors, including tobacco smoking, and in the gestational period is discussed. It is proposed to study AH candidate genes in the xenobiotics detoxifcation system, the carriage of diﬀerent variants of which can determine the sensitivity or resistance to antihypertensive pharmacotherapy, which can be useful for developing of the personalized tactics of managing patients with AH.
FUNDAMENTALISM AMONG ACEHNESE YOUTH: ACEHNESE UNIVERSITY STUDENTS’ ATTITUDES ON RELIGIOUS FUNDAMENTALISM, RIGHT-WING AUTHORITARIANISM, AND MILITIA SENTIMENTS
Authors: Taufik Alif Azadi , . Farnanda
Published: February 2019

Purpose:This study aims to measure the attitude levels of Acehnese university students on religious fundamentalism, right-wing authoritarianism, and militia sentiments and determine whether there are strong correlations between these attitudes.

Methodology:A modified Islamic and Indonesian version of the Revised Religious Fundamentalism Scale, the Right-Wing Authoritarianism Scale, and the Militia Scale were employed in an online survey.The survey gathered 308 responses and 85 of them completed.

Results:Acehnese Muslims display a 69.3%, 54% and 58% attitude level of religious fundamentalism, right-wing authoritarianism, and militia sentiments respectively, displayingattitudes significantly higher than Toronto Muslims, Hindus, Jews, United States and Canadian Christians, and Ghanaian Christians and Muslims. Religious fundamentalist attitudes correlated 72% with right-wing authoritarian attitudes and 62%with militia sentiments. Right-wing authoritarianism correlated 61% with militia sentiments.

Implications:The findings of this study are useful in understanding the interlocking social relationships of religious fundamentalism, right-wing authoritarianism, and militia sentiments. Specifically, these findings indicate the challenges the Indonesian government still face in empowering democracy and restoring the central government’s legitimacy in Aceh.

Originality:This study adapts well-known scales into a unique Indonesian and Islamic context, providing a unique perspective in the discussion of religious fundamentalism, right-wing authoritarianism, and militia sentiments. It elucidates the unique personality of Acehnese youth, whom have been under studied.
ACEHNESE COFFEE SHOPS AS PUBLIC SPACES: ACEH POLITICAL PARTICIPATION MODEL IN YOGYAKARTA
Authors: . Farnanda , Efendi David , Taufik Alif Azadi , Sulaksono Tunjung
Published: February 2019

Purpose: This paper aims to recognize a new form of political participation that is expressed by Acehnese coffee shop drinkers in supporting and criticizing local Acehnese politics, particularly those in Yogyakarta. Furthermore, this research examines the role of informal movements and clubs involved in decision-making related issues of Acehnese living in Yogyakarta.

Methodology:This is a critical qualitative approach with in-depth interviews and participatory observation over several months. Participatory observation was conducted through immersive participation in Acehnese coffee shops as public spaces and in-depth interviews with coffee shop managers, community leaders, and regular customers.

Findings: The Acehnese unique cultural and social culture of coffee drinking plays a dynamic role with Acehnese community’s relationship with politics. This culture facilitates the existence of Acehnese identity in Yogyakarta and intensifies political participation through informal discussion. Acehnese coffee shops are free and autonomous public spaces, which are collectively owned and persevered by the community.

Implication: Informal politics and local identity expressed through far political distances are interesting in Indonesia to examine social cohesion and capital nationwide. Out study also develops a model contributes to political science in Indonesian by explaining the relationship between informal and formal politics. It helps explain differences of different cultural and ethnic groups in Indonesia.

Originality: Our paper investigates the perspective of local politics within Indonesian politics. Furthermore, most contemporary political research focuses on formal and official politics, while this paper uncovers long distance informal politics embedded in unique Acehnese social culture in coffee shops. The civic contribution of Acehnese coffee shops as a public place in Indonesian democracy is convincingly established in this paper.
Plasma vs serum – does the type of biological material affects the results of parathyroid hormone determinations?
Authors: Beda-Maluga Karolina , Paczuszka Paulina , Pisarek Hanna , Świętosławski Jacek , Winczyk Katarzyna
Published: February 2019

Wstęp

Materiałem biologicznym zalecanym do oznaczania parathormonu (parathyroid hormone – PTH) jest osocze krwi żylnej pobranej na wersenian sodowo-potasowy, jednak według producentów zestawów diagnostycznych do pomiaru PTH można stosować zarówno osocze, jak i surowicę krwi.
Celem pracy było zbadanie, czy wartości PTH oznaczane w surowicy i w osoczu krwi żylnej są porównywalne.

Materiał i metody

W badaniu wykorzystano próbki krwi żylnej (osocze i surowica) uzyskane od 92 pacjentów Poradni Endokrynologicznej Uniwersyteckiego Szpitala Klinicznego im. Wojskowej Akademii Medycznej – Centralnego Szpitala Weteranów (USK im. WAM – CSW) w Łodzi. Oznaczenia hormonu wykonano na analizatorze Immulite 1000 (Siemens) zestawem iPTH.

Wyniki

Wartości PTH oznaczone w osoczu i w surowicy były jednakowe tylko w 3 przypadkach. W 46 próbkach (50%) wyższe wartości hormonu odnotowano w osoczu, a w 43 przypadkach (47%) w surowicy. Różnice bezwzględne między wartościami PTH w badanych materiałach wahały się od 0 do 38 pg/mL i zwiększały się wraz ze wzrostem stężenia hormonu. Różnice względne (procentowy stosunek różnic bezwzględnych do stężenia PTH w osoczu) wynosiły od 0 do 34,3%. Jednak zarówno różnice bezwzględne, jak i względne nie były znamienne statystycznie (p > 0,05). Po klasyfikacji stężeń – na prawidłowe, obniżone lub podwyższone – na podstawie wartości referencyjnych PTH dla danego materiału biologicznego uzyskano zgodność wyników w 83 badanych próbkach (90%).

Wnioski

Stężenia PTH oznaczane w osoczu i w surowicy nie są identyczne, ale porównywalne, a różnice w większości przypadków nie wpływają na interpretację kliniczną wyników.
Evaluation of correlation between functional shortening of hamstring and functional shortening of rectus femoris muscle in patients with anterior cruciate ligament (ACL) injury
Authors: Szlęzak Michał , Czerwik Daria , Wasilewska Magdalena , Baryluk Krzysztof , Likus Wirginia , Ficek Krzysztof
Published: February 2019

Wstęp

Więzadło krzyżowe przednie jest jedną z ważniejszych struktur stawu kolanowego. Zawiera mechanoreceptory, które dzięki wrażliwości na ruch umożliwiają odczuwanie pozycji stawowej oraz kinestezji. Uszkodzenie powoduje zmiany w funkcji kolana. W literaturze szeroko opisywane są pozycje dotyczące translacji oraz wysięku, ale coraz większą uwagę zwraca się na funkcję mięśni: siłę i sztywność. Urazowi towarzyszą uczucie niestabilności kolana i ból przy obciążeniu kończyny urazowej. Występuje również wysięk, który stopniowo ogranicza zakres ruchu w stawie, wzmaga dolegliwości bólowe oraz wtórnie może wpłynąć na funkcje mięśni. Skrócenie funkcjonalne mięśni kulszowo-goleniowych może być objawem nieprawidłowej kontroli w obrębie stawu kolanowego.

Materiał i metody

Badania zostały przeprowadzone na grupie 46 mężczyzn w wieku 18–46 lat (średnia wieku 25 lat; SD 6), u których stwierdzono zerwanie więzadła krzyżowego przedniego (anterior cruciate ligament – ACL) po 5–52 tygodniach od urazu (średnia 20 tygodni; SD 15). Dokonano pomiaru długości czynnościowej mięśni kulszowo-goleniowych oraz prostego uda zarówno w sposób bierny, jak i czynny. W tym celu użyto inklinometru Saundersa, który pozwala dokonać pomiaru do 1°.

Wyniki

Analiza statystyczna wykazała istotną statystycznie ujemną korelację między skróceniem funkcjonalnym mięśni kulszowo-goleniowych a prostym uda w kończynie zdrowej w badaniu czynnym oraz biernym.

Wnioski

Wraz ze wzrostem funkcjonalnego skrócenia mięśni kulszowo-goleniowych zmniejsza się skrócenie funkcjonalne mięśnia prostego uda zarówno w badaniu czynnym, jak i biernym w kończynie zdrowej. Wzrostowi skrócenia funkcjonalnego mięśni kulszowo-goleniowych towarzyszy spadek skrócenia mięśnia prostego uda w kończynie z zerwanym ACL zarówno w badaniu czynnym, jak i biernym.
ЛИТОЛОГО-ПЕТРОГРАФИЧЕСКИЕ ОСОБЕННОСТИ КОЛЛЕКТОРОВ БАЖЕНОВСКОЙ СВИТЫ НА ЮГО-ВОСТОКЕ ЗАПАДНО-СИБИРСКОЙ ПРОВИНЦИИ (ТОМСКАЯ ОБЛАСТЬ)
Authors: (Natalya M. Nedolivko) Недоливко Наталья Михайловна , (Tatyana G. Perevertaylo) Перевертайло Татьяна Геннадьевна
Published: February 2019

Актуальность исследования продиктована необходимостью расширения ресурсной базы Западно-Сибирского региона, в том числе и Томской области, за счет вовлечения в разработку трудноизвлекаемых запасов нефти, сосредоточенных в нефтематеринской баженовской свите (титон-берриасс). Цель исследования: выявить особенности литологического состава и строения баженовского резервуара на прогнозно-перспективной для добычи нефти территории Томской области. Объект исследования: битуминозные отложения баженовской свиты, вскрытые бурением на локальном поднятии в Тамратской впадине (Нюрольский осадочный бассейн), Пудинский нефтегазоносный район Западно-Сибирской нефтегазоносной провинции (Томская область), из которых получен приток нефти. Методы: геофизические исследования, макрокопическое описание керна, петрографический и рентгенофазовый анализы. Результаты. В продуктивном разрезе баженовской свиты выявлены границы распространения разуплотненных пород – коллекторов нефти и газа. Приведена их литологическая характеристика, установлены текстурно-структурные особенности и вещественный состав. Выделено семь литотипов: 1 – алеврито-силицитовые аргиллиты со следами жизнедеятельности илоядных животных и трубчатыми раковинами; 2 – битуминозные силицито-аргиллиты с кремнистыми прослойками и остатками кремниевых водорослей; 3 – битуминозные силициты глинистые радиоляриевые; 4 – битуминозные силициты радиоляриевые с трубчатыми раковинами и кремнистыми прослойками; 5 – битуминозные аргиллиты силицитовые радиоляриевые со следами биотурбации и остатками криноидей; 6 – силицит-аргиллиты радиоляриевые с остатками водорослей; 7 – силицит-глинисто-карбонатные породы. Выяснено, что накопление осадков баженовской свиты осуществлялось при стабильно низкодинамичном застойном режиме седиментации в относительно глубоководных морских условиях восстановительной геохимической среды. Установлена связь минерального состава и содержания сапропелевого органического вещества с трансгрессивно-регрессивной цикличностью осадконакопления: наиболее обогащен кремнистым материалом и органическим веществом литотип 4, максимальное количество этих компонентов приходится на точку инверсии трансгрессивно-регрессивного цикла. Баженовский коллектор имеет смешанное происхождение, формировался при стадиальном литогенезе (седиментогенез–катагенез) и постстадиальных процессах. Пустотное пространство в нем представлено ультратонкими биопустотами и порами, межслоевыми и внутрислоевыми трещинами.
Pengaruh Penambahan Madu Dalam Pakan Induk Jantan Lobster Air Tawar Red Claw (Cherax quadricarinatus) Terhadap Rasio Jenis Kelamin Larva [The Effect Of Suplementation Of Honey In Feed Of Male Mains Fresh Water Cray Fish Red Claw (Cherax quadricarinatus) To Larva Sex Ratio ]
Authors: Mukti A.Taufiq , Mubarak A.Shofy , Ermawan Adde
Published: February 2019

Abstract The aim of this research was to know the effect of honey supplementation in feed of male main freshwater cray fish red claw to larva sex ratio and the level of survival of freshwater cray fish and to get the best doses of honey to give highest male sex ratio. This research was done at Laboratory of Fishery Education, Fishery and Marines Faculty, Airlangga University in October 2007 until March 2008. Methods of this research was experimental and used completely randomized designed with 7 treatments. Giving of honey with differences doses were A ( 0 ml/kg) control, B ( 50 ml /kg), C ( 100 ml /kg), D ( 150 ml /kg), E ( 200 ml /kg), F ( 250 ml/kg) and G ( 300 ml/kg) which each treatment got three times replicated. Data processed by Analysis of Variance (ANOVA). The result of this research showed that supplementation of honey in feed male main freshwater cray fish red claw not significantly different to larva sex ratio and survival rate of freshwater cray fish. Otherwise, result of the supplementation of honey in feed of male main freshwater cray fish showed the increasingly male sex ratio of freshwater cray fish red claw larvae.
Kultur Sel Otak Ikan Kerapu Bebek (Cromileptes altivelis) dengan Menggunakan Media L-15, MEM Dan TCM
[Cell Culture Brain Of Chromileptes altivelis With Medium L-15, MEM And TCM].
Authors: Suprapto Hari , Diantiwi Nunik
Published: February 2019

Abstract Humpback grouper is high value commodity fish. The demand of domestic and foreign markets increases every year. The obstacles of Humpback grouper fish cultivation is a disease caused by Viral Nervous Necrosis (VNN). The virus attacks the brain leading to mass death. To prevent the disease, a research on VNN virus has to be started. The problem of the research is the lack of culture of fish tissue. Virus have specific location to tissue cell and species to be infected. Thereofore, first step must be done is growth from tissue cell which infection of virus. Cell culture is a technique to isolate cell, protoplasm, tissue or organ to be grown in a sterile condition with nutrition containing growth hormone to promote multiplication. Cell culture technique can be used in many basic applications in cell biology. The purpose of this research is to develop cell culture from Grouper fish brain for VNN research. The hope is culture can grow and develop well.Research executed at June until Desember 2009 in Laboratory In Vitro of Veterinari Faculty of Airlangga University and Laboratory Gastroenteritis Institute of Tropical Disease, Airlangga University, Surabaya. The result showed that brain cell culture of humpback grouper fish can be grow and multiply well in all three media, media L-15 cell growth was faster compared to media MEM and TCM.
Prevalensi dan Derajat Infeksi Dactylogyrus sp. pada Insang Benih Bandeng (Chanos chanos) di Tambak Tradisional, Kecamatan Glagah, Kabupaten Lamongan
[Prevalence and Infection Level of Dactylogyrus sp. on Gill of Milkfish Juvenile (Chanos chanos) in Traditional Pond, Glagah Subdistrict, Lamongan Residence]
Authors: Mas’ud Faisol
Published: February 2019

Abstract The aim of research was to know prevalence and infection level of Dactylogyrus sp. on gill of milkfish juvenile (Chanos chanos) in traditional pond, Glagah Subdistrict, Lamongan Residence. It was using sample of 5 milkfish juvenile and for each treatment three replication. Researh design was Complete Random Design and continued by Analysis of Variance (ANOVA) and Least Significant Difference (LSD) 5%. The result showed that prevalence of Dactylogyrus sp. on gill of milkfish juvenile in Dalung village and Pedurungan village were significantly of 53.33% and 86.67%, respectively. Furthermore, infection level of Dactylogyrus sp. on gill of milkfish juvenile were significantly on all of area.
Pengelolaan Limbah pada Pabrik Pengolahan Ikan di PT. Kelola Mina Laut Gresik
[Waste Treatment at Fish Processing Company in Kelola Mina Laut Incorporated Gresik East Java Province]
Authors: Desi Wulansari Putri
Published: February 2019

AbstractIndustrial waste comes from the end of product process as a solid or liquid waste. Avoiding the negative effects of industrial waste, each industry must complete their company with wastewater treatment plant. This Field Job Practice was done at Kelola Mina Laut, Inc., located in Randu Agung Village, Kebomas District, Gresik th thResidence, East Java Province on August 4 – September 13 as a mean to observe, learn and to know Kelola Mina Laut, Inc. wastewater treatment method. Work method which used in Field Job Practice was descriptive method with data collection technique, active participation, interview and literature study. The wastewater treatment plant of Kelola Mina Laut, Inc. consists of one collector batch unit, two aeration batch units, one sedimentation batch unit, and one contol batch unit. Before loosing the effluent to the water stream, wastewater passes through the control batch to know about the wastewater quality. After passing through the treatments, wastewater that loosing upon the environment will have a good quality and appropriate with Environment Quality Standard.
Studi Identifikasi dan Prevalensi Endoparasit pada Saluran Pencernaan Ikan Kerapu Tikus (Cromileptes altivelis) di Keramba Jaring Apung Unit Pengelola Budidaya Laut Situbondo, Jawa Timur
[Prevalence Study And Identification Of Endoparasites On Humpback Grouper Gastrointestinal (Cromileptes altivelis) In Floating Net Cage Marine Culture Management Unit Situbondo, East Java]
Authors: Kismiyati Kismiyati , Kusdarwati Rahayu , Ulkhaq Mohammad Faizal
Published: February 2019

Abstract Humpback grouper (Cromileptes altivelis) or also known as polkadot grouper is one of the excellent fish commodities in Indonesia which has trading value reached $ 90-150/kg. As a consumed fish, humpback grouper are needed for beverage at luxuries restaurant and hotel in the world. As major export commodities in Indonesia, since the 1990's the techniques of grouper cultivation in float net rats are enhanced to fulfill international market demand. Humpback grouper fish cultivation techniques in float net cages cannot be separated from the problems in any cultivate activities, one of the causes is a disease and one of the diseases that rushed humpback grouper is parasite. The disadvantages that caused by the parasite is fish’ weight loss. The purpose of this study is to determine the types of endoparasites that attacked the digestive system of humpback grouper (Cromileptes altivelis) in float net cages and also to discuss the prevalences of endoparasites that attack the digestive of humpback grouper (Cromileptes altivelis) in float net cages. The method of this research is descriptive. The sampling was done in once for 60 fishes with the length range of fish is around 15-20 cm and 4 months old from the float net. The number of each fishes samples that were taken 5-10% from the total of population, where the number of humpback grouper populations in each raft float net are 200 fishes. The main parameters that observed in this study were endoparasites identification species that attack the digestive system of humpback grouper (Cromileptes altivelis) in float net cages and the prevalence rates for each endoparasites. The supporting parameters in this study were the water quality in float net cages which includes temperature, pH and salinity are measured during the sample taken. The results showed that 60 samples taken from four float nets 6 fishes were positive infected endoparasitic of trematodes and nematodes, besides 4 fishes are positive infected worms Echinostoma which belong to the class of Trematodes and 2 fishes infected worms Camallanus carangis that belongs to the class Nematoda. The prevalence values for each of the worm are 6.67% for Echinostoma and 3.33% for Camallanus carangis. The research concern the identification and prevalence of endoparasites in Humpback grouper at hatchery is needed to early finding out the attack of endoparasites and to break the flow of endoparasitic spreading.
Potensi dan Kesesuaian Lahan Budidaya Rumput Laut (Kappaphycus alvarezii) Di Sekitar Perairan Kabupaten Wakatobi Provinsi Sulawesi Tenggara
[Potential And Suitability Of Land Seaweed Farming (Kappaphycus alvarezii) Water Around The District Wakatobi Southeast Sulawesi]
Authors: Paena Mudian , Rangka Nur Ansari
Published: February 2019

Abstract Ragulasi government of commodities in the field of aquaculture developed into a major trigger of the mushrooming development of seaweed farming. (Kappaphycus alvarezii) in most coastal areas in Indonesia, not least in district Wakatobi Southeast Sulawesi. In development, the local government in this case the relevant agencies do not yet have data on potential and suitability of the land to be used as a reference in determining the development strategy. Based on this research has been done to calculate the potential and aimed to determine the suitability of land for seaweed cultivation in the district of Wakatobi. The study was conducted with the direct survey method to extract the characteristics of the study sites for hydro-oceanographic dynamics which is closely linked with the growth of seaweed. Data were tabulated and the field measurement results were analyzed descriptively, whereas spatial analysis for thematic map creation and distribution of the potential suitability of land is done by applying the technology of geographic information systems (GIS) utilizing software Arc-View 3.3. The results showed that the distribution characteristics of hydro-dynamics-osenaografi around Wakatobi waters are bersubstrat sand, coral sand and coral; flow rate between 0.011 to 0.07 m / sec; brightness from 2.5 to 15 meters; depth of 2.5 to 13.7 meters; temperature of 29.42 to 30.2 ° C; pH 7.58 to 8.23; salinity 34.95 to 36.88 ppt; okesigen dissolved from 6.06 to 6.25 m / L; total organic matter (BOT) from 35.52 to 42.6 mg / L; ammonia (NH3) from 0.0096 to 0.0136 mg / L; Nitrate (NO3) from 0.003 to 0.1593 mg / L; Nitrite (NO2) 0.001 - 0.0085 mg / L; Phosphate (PO4) 0.045 to 0.198 mg / L; Iron (Fe) 0.035 to 0.652 mg / L; and Total Suspendet Solit (TSS) 34-125 mg / L. The potential for seaweed cultivation of 17,391.87 hectares of land in accordance with the level of compliance and inappropriate 9,858.62 7,533.25 ha ha, whereas eksisiting 9,614.42 ha of land.
Uji Aktivitas Antifungi Ekstrak Rimpang Kencur (Kaempferia galanga L.) Terhadap Saprolegnia sp. Secara In Vitro
[Antifungal Activity Test Of Kaempferia galanga L. Extract To Saprolegnia sp. By In Vitro]
Authors: Dewa Ketut Meles Rahayu Kusdarwati, Ayu Ratnaningtyas,
Published: February 2019

Abstract Saprolegnia sp. is a fungus that causes the Saprolegniasis disease can infection eggs and fresh water fish. Treatment Saprolegniasis done using chemical drugs, however the use of drugs is bad for the environment and biota. The purpose of the research was to determined the antifungal activity include a minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) from Kaempferia galanga L. to Saprolegnia sp. by in vitro. This research used 9 different concentrations of Kaempferia galanga L extract were 50%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0, 39%, 0.2%, positive control used H2O2 3% and negative control used DMSO 10%. The results showed that the extract of Kaempferia galanga L had an antifungal activity were inhibits and kill with minimum inhibitory concentration (MIC) was 0.39% equivalen with 3,9 mg/ml and minimu fungicidal concentration (MBC) was 1.56% equivalen with 15,6 mg/ml. The existence of antifungal activity against Saprolegnia sp. by in vitro caused by some active compounds from the extracts of the Kaempferia galanga L. are polyphenolic compounds, flavonoin, saponins and essential oils.
Pengaruh Pemberian Pakan Beryodium terhadap Pertumbuhan dan Kandungan Yodium Benih Ikan Mas (Cyprinus carpio)
[Effect Of Iodized Feeding On The Growth And Content Of Iodine In Carpfish Seed (Cyprinus carpio) ]
Authors: Agustono Agustono , C. W Lia Enggar , Lamid Mirni
Published: February 2019

Abstract Carpfish culure have a quiet effort to profit and developed by people, because it have characteristic like adapted with the environtment, have rapid growth and resistance of disease. Carpfish cultivation is often cultivated in mountainous area far from sources of iodine (Cholik etc, 2002 ; Susanto, 1997). This causes iodine contain is very low, carp fish has only 1.7 mg/100g iodine contain. That amount is not sufficient people nutrition needs. Purpose of this study was to increase the iodine contain and growth. Experiment have been conducted in April until May 2011, for 1 month in Aquaculture Development Laboratory Hang Tuah University, Surabaya. Data retrieval was done by observation, direct participation and documentation thechnique. The research method is an experiment method with a completely randomized desigen consisted of 5 treatment and 4 replication, at a dose of 0 mg / kg of feed, 20 mg / kg of feed, 40 mg / kg of feed, 60 mg / kg feed and 80 mg / kg of feed. Each treatment used 15 carp seed (Cyprinus carpio) measuring 5-8 cm is maintained for 30 days. The parameter used include the primary parameter and parameter support. The main parameter of the growth and content the iodine, while supporting the water quality parameter. Result of this study were analized by using analysis of various with an error rate 5% later, followed by Duncan multiple rate test. Result of this study indicate that feeding iodized can increased the growth in wight and iodine content of carp.
Eksplorasi Bakteri Kandidat Probiotik di Lumpur Hutan Mangrove Wonorejo
[Exploration The Candidate Probiotic Bacteria In Mangrove Mud Wonorejo]
Authors: Kusdarwati Rahayu , Pratiwi Indah , Tjahjaningsih Wahju
Published: February 2019

AbstractMangrove as one of the bacterial community development area, one of which is a probiotic bacterium. Exploration of probiotic bacteria in the mud of mangrove forest has not been done, especially in the mud in the mangrove forest Wonorejo, Rungkut-Surabaya.  This study aims to determine the candidate probiotic bacteria species found in mangrove forests Wonorejo. This research method is descriptive in the form of sludge sampling surveys at five different points area. Sampling points are T1, T2, T3, T4 and T5. T1 is an area of mangrove forest near Wonorejo milkfish. T2 is a region of southern estuarine mangrove forest near Wonorejo milkfish. T3 is a region of northern estuary mangrove forest Wonorejo near sewerage drains Wonorejo citizens. T4 is a region of southern estuarine mangrove forest Wonorejo and T5 is a region of northern estuarine mangrove forest Wonorejo. Fifth sludge samples performed a series of tests in the identification bekteri Hall Fish Quarantine, Quality Control and Safety of Fishery Products Class 1 Juanda. Data were analyzed by descriptive identification results by describing the data distribution of probiotic bacteria at each sampling point area Wonorejo mangrove mud.  The results showed that probiotic candidate bacterias found were Bacillus sp., B. subtilis, B. licheniformis, Pseudomonas sp., P. pseudomallei, Vibrio alginolyticus and Microccus sp.
Pengukuran Kecernaan Protein Kasar, serat Kasar, Lemak Kasar, dan Energi Pada Pakan Komersial Ikan Gurami (Osphronemus gouramy) dengan Menggunakan Teknik Pembedahan [Digestibility Value Of Crude Fiber and Nitrogen Free Extract (Nfe) for Different Commercial Feed In Gouramy (Osphronemus goramy Lac.) Using Surgical Technique]
Authors: Agustono Agustono
Published: February 2019

Abstract Gouramy (Osphronemus goramy Lac.) belongs to a group of freshwater fish that have an important economic value and has been cultivated as other economic fish. Increase of biomass production in an aquaculture should consider feeding factors. Not every components in the feed can be absorbed in the digestive process. Parts that can not be absorbed by the body will be issued in the form of the feces so it can be determined the value of the digestibility of a feed material. Crude fiber and nitrogen free extract (NFE) digestibilty value is kind of nutrition that can be determinated. This study aims to determine the digestibility value of crude fiber and nitrogen free extract (NFE) in different commercial feed mills in gouramy (Osphronemus goramy Lac.). This study used 3 treatments, P1 ( commercial feed A), P2 (commercial feed B) and P3 (commercial feed C) with 6 replications. Digestibility value measurement method used was taking feces with surgical techniques. Feces retrieval method aims to minimize water contact with feces. The research design used in this study is completely randomized design. Observed variables are digestibility of crude fiber and nitrogen free extract (NFE). Based on the results of the study can be seen that in the used of diffetent commercial feed there is no significant difference (p> 0.05) on the digestibility value of crude fiber and nitrogen free extract.
Monitoring Hasil Perikanan dengan Alat Tangkap Pancing Tonda di Pelabuhan Perikanan Nusantara Prigi, Kabupaten Trenggalek, Propinsi Jawa Timur [Monitoring Of Fishery With Fishing Gear Troling Line In The Prigi Nusantara Fishing Port, Trenggalek District, East Java Province]
Authors: Manan Abdul , Putra Fajar Nurcahya Dwi
Published: February 2019

Abstract Indonesia is a maritime country that has great potential of capture fisheries. There are various methods to conduct of fishing operations by fisherman, one of them is troling line. Troling line is a fishing equipment used to capture pelagic fish. The purpose of this study case is to know the technique of fishing operations by using a troling line, to find out the capture by a troling line, and to identify obstacles in the activities of fishing using a troling line. Monitoring was carried out in territory authority Prigi Nusantara Fishing Port, Watulimo, Trenggalek, East Java. This activities was held from July 19th to September 1st 2010. The method used are descriptive method with the retrieval of data includes primary data and secondary data. Data were collected by active participation, observation, and literature. Monitoring in production of troling line operation can be used to determine the effectiveness of troling line and overall process of catching up to the handling of post-catch yield. The process of fishing with the troling line respectivelly settings stage, trolling, and hauling. The kind of pelagic fish capture are Thunnus albacore, Katsuwonus pelamis, and Euthynus affinis. Total of capture is 4.556 kg. The results of this monitoring indicate that the troling line less effective in fishing operations. Barriers faced by the fishermen at PPN Prigi in conducting fishing operations is a technical problem and natural problem. Technical problem happening such as destruction of fishing gear during fishing operations.
Pengaruh Pemberian Minyak Atsiri Daun Bandotan (Ageratum conyzoides) Dalam Transportasi Tertutup Benih Ikan Koi (Cyprinus Carpio)
[The Effects Of Bandotan Leave’s (Ageratum conyzoides) Essential Oil within Closed System Transportation of Koi Carp (Cyprinus carpio) ]
Authors: Sulmartiwi Laksmi , Dantika Ida Bagus Putu Oka , Triastuti Rr. Juni
Published: February 2019

Abstract In the ornamental fish business, transportation has an important role to distributing fish up into the costumers. The distance between the farm and the customers location, require a transportation method which can maintain the quality and quantity of ornamental fish during the transportation. The biggest challenge in live fish closed system transportation is to reduce the stress level of fish during the process. According to Harmon (2009), fish are often exposed to multiple stressor during the transportation. Stressed fish can be seen through the increasing of tachiventilation (rapid opercula movement) and blood glucose levels (Evans and Claiborne, 2006). One of the method to reduce stress of fish during transportation is using essential oils from Bandotan’s (A. conyzoides) leaves. A. conyzoides essential oils know has an analgesic activity (Okunade, 2002; Shekhar and Anju, 2012; Singh et al., 2013). A chemical compound with known analgesic effect, used in human and veterinary medicine as local analgesic dan be used to fish for the purpose of sedation, immobilization, and analgesia as well as general anaesthesia (Zahl and Samuelsen, 2012). This research was conducted to see the effect of A.conyzoides essential oils toward the survival rate, blood glucose levels, and tachyventilation of koi carp juveniles during closed system transportation. This research using completely randomized design method which consist of 5 different treatments with 4 replications. Treatment that used in this research is the variation of administration doses of A. conyzoides essential oils during the koi carp juvenile closed system transportation. The treatment such as A (5 ppm), B (10 ppm), C (15 ppm), D (20 ppm), and E (0 ppm). Main parameter of this research is survival rate, blood glucose levels, and tachyventilation of koi carp juvenile during 8 hours transportation. The result shows that A. conyzoides essential oils give a significant result toward survival rate, blood glucose levels, and tachiventilation of koi carp juvenile during transportation. There is no mortality in treatment A, B, and E, during transportation. The lowest blood glucose levels and tachiventilation found in treatment A (5 ppm).
Pengaruh Penambahan Crude Fish Oil (CFO) pada Pakan Udang Vaname (Litopenaeus vannamei) Terhadap Kandungan Kolesterol dan Retensi Lemak Daging [ Influence of Addition Crude Fish Oil (CFO) in White Shrimp (Litopenaeus vannamei) Feed to Cholesterol Content and Fat Retention In Meat ]
Authors: Agustono Agustono , Lokapirnasari Widya Paramitha , Riyadh Azharur
Published: February 2019

Abstract White shrimp are aquatic organisms consumed by many people and a source of animal protein and fat needed by humans. Shrimp also require fat as a source of energy and metabolic processes to maximize growth. White shrimp meat contains high cholesterol so that it becomes an obstacle to consume white shrimp and high cholesterol in feed can affect the growth of shrimp. This problem can be solved by adding Crude Fish Oil (CFO) which contain high omega-3 fatty acids in white shrimp feed to decrease cholesterol content and source of dietary fat to increase fat retention in meat. The research problem is whether the addition of CFO in white shrimp feed effect on the decrease cholesterol content and increase of fat retention in meat. This research aims to determine the effect of CFO in white shrimp feed to decrease cholesterol and increase of fat retention in meat. The method used is experimental method with Completely Randomized Design (CRD) as the experimental design. The treatment used is different persentage CFO addition to white shrimp feed, ie P0 (0%), P1 (2%), P2 (4%), P3 (6%) and P4 (8%) each treatment is repeated 4 times. Analysis of the data using Analysis of Varians (ANOVA) and to determine the best treatment performed Duncan's Multiple Range Test. Results showed that the addition of CFO in feed give significant effect (p <0.05) to decrease the cholesterol content in meat. The best average decrease of cholesterol content obtained at P3 (135.22 mg/100 g) and P4 (133.51 mg/100 g). Addition of CFO in feed did not give significant effect (p>0.05) to increase fat retention in meat.
Perbandingan Viskositas, Titik Nyala dan Titik Beku Biodiesel dari Rumput Laut (Eucheuma denticulatum), Minyak Ikan Lemuru (Sardinellla longiceps) dan Biodiesel Komersil [ The Comparison of Viscosity, Flash Point and Freeze Point Biodiesel of Seagrass (Eucheuma denticulatum), Sardinella Oi]
Authors: Rahardja Boedi Setya , Satyantini Woro Hastuti , Wijayanto Andry
Published: February 2019

Abstract Necessity of fuel are increasing rapidly nowadays. But oil is an irreversible product, so it is required to look for other alternative fuels, especially from renewable materials. Currently being developed technology that uses biodiesel as renewable alternative fuel. The aim of this research to determine whether oil and seaweed Eucheuma denticulatum and Lemuru fish oil can be used as materials for biodiesel. This research was conducted by observing and comparing the flash point, freeze point, and viscosity of each treatments. The Method in this research is experimental method and supported by panelist. There were 9 treatments in this study, there are P0 (Biodiesel commercial), P1 (Biodiesel Eucheuma denticulatum 100 %), P2 (Biodiesel Eucheuma denticulatum 75% + Biodiesel commercial 25%), P3 (Biodiesel Eucheuma denticulatum 50% + Biodiesel commercial 50%), P4 (Biodiesel Eucheuma denticulatum 25% + Biodiesel commercial 75%), P5 (Biodiesel Sardinella oil 100 %), P6 (Biodiesel Sardinella oil 75% + Biodiesel commercial 25%), P7 (Biodiesel Sardinella oil 50% + Biodiesel commercial 50%), and P8 (Biodiesel Sardinella oil 25% + Biodiesel commercial 75%). Each treatments was repeated 3 times. The main parameter observed was flash point, freeze point, and viscosity in every treatment. The data analysis used was non-parametric test with Friedman test and continued with Wilcoxon test. The results of this study indicate that the best of the nine treatments is P8 which is a mixture of lemuru fish oil biodiesel 25% and 75% biodieesel with the specifications freezing point -3oC, viscosity of 4.02 mm2 / s, and the flash point of 47.9oC.