bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Ferrocenyl 4-(Imino)-1,4-Dihydro-quinolines on Xenopus laevis Prophase I - Arrested Oocytes: Survival and Hormonal-Induced M-Phase Entry
Authors: Marchand Guillaume , Wambang Nathalie , Pellegrini Sylvain , Molinaro Caroline , Martoriati Alain , Bousquet Till , Markey Angel , Lescuyer-Rousseau Arlette , Bodart Jean-François , Cailliau Katia , Pelinski Lydie , Marin Matthieu
Published: April 2020

Xenopus oocytes were used as cellular and molecular sentinels to assess the effects of a new class of organometallic compounds called ferrocenyl dihydroquinolines that have been developed as potential anti-cancer agents. One ferrocenyl dihydroquinoline compound exerted deleterious effects on oocyte survival after 48 h of incubation at 100 μM. Two ferrocenyl dihydroquinoline compounds had an inhibitory effect on the resumption of progesterone induced oocyte meiosis, compared to controls without ferrocenyl groups. In these inhibited oocytes, no MPF (Cdk1/cyclin B) activity was detected by western blot analysis as shown by the lack of phosphorylation of histone H3. The dephosphorylation of the inhibitory Y15 residue of Cdk1 occurred but cyclin B was degraded. Moreover, two apoptotic death markers, the active caspase 3 and the phosphorylated histone H2, were detected. Only 7-chloro-1-ferrocenylmethyl-4-(phenylylimino)-1,4-dihydroquinoline (8) did not show any toxicity and allowed the assembly of a histologically normal metaphase II meiotic spindle while inhibiting the proliferation of cancer cell lines with a low IC50, suggesting that this compound appears suitable as an antimitotic agent.
Modeling Neurodegenerative Disorders in Drosophila melanogaster
Authors: Bolus Harris , Crocker Kassi , Boekhoff-Falk Grace , Chtarbanova Stanislava
Published: April 2020

Drosophila melanogaster provides a powerful genetic model system in which to investigate the molecular mechanisms underlying neurodegenerative diseases. In this review, we discuss recent progress in Drosophila modeling Alzheimer’s Disease, Parkinson’s Disease, Amyotrophic Lateral Sclerosis (ALS), Huntington’s Disease, Ataxia Telangiectasia, and neurodegeneration related to mitochondrial dysfunction or traumatic brain injury. We close by discussing recent progress using Drosophila models of neural regeneration and how these are likely to provide critical insights into future treatments for neurodegenerative disorders.
An efficient Screening System in Yeast to Select a Hyperactive piggyBac Transposase for Mammalian Applications
Authors: Wen Wen , Song Shanshan , Han Yuchun , Chen Haibin , Liu Xiangzhen , Qian Qijun
Published: April 2020

As non-viral transgenic vectors, the piggyBac transposon system represents an attractive tool for gene delivery to achieve a long-term gene expression in immunotherapy applications due to its large cargo capacity, its lack of a trace of transposon and of genotoxic potential, and its highly engineered structure. However, further improvements in transpose activity are required for industrialization and clinical applications. Herein, we established a one-plasmid effective screening system and a two-step high-throughput screening process in yeast to isolate hyperactive mutants for mammalian cell applications. By applying this screening system, 15 hyperactive piggyBac transposases that exhibited higher transpose activity compared with optimized hyPBase in yeast and four mutants that showed higher transpose activity in mammalian cells were selected among 3000 hyPBase mutants. The most hyperactive transposase, bz-hyPBase, with four mutation sites showed an ability to yield high-efficiency editing in Chinese hamster ovarian carcinoma (CHO) cells and T cells, indicating that they could be expanded for gene therapy approaches. Finally, we tested the potential of this screening system in other versions of piggyBac transposase.
Simultaneous Monitoring of Multi-Enzyme Activity and Concentration in Tumor Using a Triply Labeled Fluorescent In Vivo Imaging Probe
Authors: Tam Jenny , Pilozzi Alexander , Mahmood Umar , Huang Xudong
Published: April 2020

The use of fluorescent imaging probes that monitor the activity of proteases that experience an increase in expression and activity in tumors is well established. These probes can be conjugated to nanoparticles of iron oxide, creating a multimodal probe serving as both a magnetic resonance imaging (MRI) agent and an indicator of local protease activity. Previous works describe probes for cathepsin D (CatD) and metalloproteinase-2 (MMP2) protease activity grafted to cross-linked iron oxide nanoparticles (CLIO). Herein, we have synthesized a triply labeled fluorescent iron oxide nanoparticle molecular imaging (MI) probe, including an AF750 substrate concentration reporter along with probes for cathepsin B (CatB) sand MMP2 protease activity. The reporter provides a baseline signal from which to compare the activity of the two proteases. The activity of the MI probe was verified through incubation with the proteases and tested in vitro using the human HT29 tumor cell line and in vivo using female nude mice injected with HT29 cells. We found the MI probe had the appropriate specificity to the activity of their respective proteases, and the reporter dye did not activate when incubated in the presence of only MMP2 and CatB. Probe fluorescent activity was confirmed in vitro, and reporter signal activation was also noted. The fluorescent activity was also visible in vivo, with injected HT29 cells exhibiting fluorescence, distinguishing them from the rest of the animal. The reporter signal was also observable in vivo, which allowed the signal intensities of the protease probes to be corrected; this is a unique feature of this MI probe design.
Pathogenic Roles of Autoantibodies and Aberrant Epigenetic Regulation of Immune and Connective Tissue Cells in the Tissue Fibrosis of Patients with Systemic Sclerosis
Authors: Tsai Chang-Youh , Hsieh Song-Chou , Wu Tsai-Hung , Li Ko-Jen , Shen Chieh-Yu , Liao Hsien-Tzung , Wu Cheng-Han , Kuo Yu-Min , Lu Cheng-Shiun , Yu Chia-Li
Published: April 2020

Systemic sclerosis (SSc) is a multi-system autoimmune disease with tissue fibrosis prominent in the skin and lung. In this review, we briefly describe the autoimmune features (mainly autoantibody production and cytokine profiles) and the potential pathogenic contributors including genetic/epigenetic predisposition, and environmental factors. We look in detail at the cellular and molecular bases underlying tissue-fibrosis which include trans-differentiation of fibroblasts (FBs) to myofibroblasts (MFBs). We also state comprehensively the pro-inflammatory and pro-fibrotic cytokines relevant to MFB trans-differentiation, vasculopathy-associated autoantibodies, and fibrosis-regulating microRNAs in SSc. It is conceivable that tissue fibrosis is mainly mediated by an excessive production of TGF-β, the master regulator, from the skewed Th2 cells, macrophages, fibroblasts, myofibroblasts, and keratinocytes. After binding with TGF-β receptors on MFB, the downstream Wnt/β-catenin triggers canonical Smad 2/3 and non-canonical Smad 4 signaling pathways to transcribe collagen genes. Subsequently, excessive collagen fiber synthesis and accumulation as well as tissue fibrosis ensue. In the later part of this review, we discuss limited data relevant to the role of long non-coding RNAs (lncRNAs) in tissue-fibrosis in SSc. It is expected that these lncRNAs may become the useful biomarkers and therapeutic targets for SSc in the future. The prospective investigations in the development of novel epigenetic modifiers are also suggested.
Behҫet’s Disease, and the Role of TNF-α and TNF-α Blockers
Authors: van der Houwen Tim , van Laar Jan
Published: April 2020

In this both narrative and systematic review, we explore the role of TNF-α in the immunopathogenesis of Behçet’s disease (BD) and the effect of treatment with TNF-α blockers. BD is an auto-inflammatory disease, characterized by recurrent painful oral ulcerations. The pathogenesis of BD is not yet elucidated; it is assumed that TNF-α may play a key role. In the narrative review, we report an increased production of TNF-α, which may be stimulated via TLR-signaling, or triggered by increased levels of IL-1β and IFN-γ. The abundance of TNF-α is found in both serum and in sites of inflammation. This increased presence of TNF-α stimulates T-cell development toward pro-inflammatory subsets, such as Th17 and Th22 cells. Treatment directed against the surplus of TNF-α is investigated in the systematic review, performed according to the PRISMA guideline. We searched the Pubmed and Cochrane database, including comparative studies only. After including 11 studies, we report a beneficial effect of treatment with TNF-α blockers on the various manifestations of BD. In conclusion, the pivotal role of TNF-α in the immunopathogenesis of BD is reflected in both the evidence of their pro-inflammatory effects in BD and in the evidence of the positive effect of treatment on the course of disease in BD.
Role of the Furosemide Stress Test in Renal Injury Prognosis
Authors: Coca Armando , Aller Carmen , Reinaldo Sánchez Jimmy , Valencia Ana Lucía , Bustamante-Munguira Elena , Bustamante-Munguira Juan
Published: April 2020

Risk stratification and accurate patient prognosis are pending issues in the management of patients with kidney disease. The furosemide stress test (FST) has been proposed as a low-cost, fast, safe, and easy-to-perform test to assess tubular integrity, especially when compared to novel plasma and urinary biomarkers. However, the findings regarding its clinical use published so far provide insufficient evidence to recommend the generalized application of the test in daily clinical routine. Dosage, timing, and clinical outcomes of the FST proposed thus far have been significantly different, which further accentuates the need for standardization in the application of the test in order to facilitate the comparison of results between series. This review will summarize published research regarding the usefulness of the FST in different settings, providing the reader some insights about the possible implications of FST in clinical decision-making in patients with kidney disease and the challenges that research will have to address in the near future before widely applying the FST.
In Utero Exposure to Bisphenol a Promotes Mammary Tumor Risk in MMTV-Erbb2 Transgenic Mice Through the Induction of ER-erbB2 Crosstalk
Authors: Ma Zhikun , Parris Amanda B. , Howard Erin W. , Davis Meghan , Cao Xia , Woods Courtney , Yang Xiaohe
Published: April 2020

Bisphenol A (BPA) is the most common environmental endocrine disrupting chemical. Studies suggest a link between perinatal BPA exposure and increased breast cancer risk, but the underlying mechanisms remain unclear. This study aims to investigate the effects of in utero BPA exposure on mammary tumorigenesis in MMTV-erbB2 transgenic mice. Pregnant mice were subcutaneously injected with BPA (0, 50, 500 ng/kg and 250 µg/kg BW) daily between gestational days 11–19. Female offspring were examined for mammary tumorigenesis, puberty onset, mammary morphogenesis, and signaling in ER and erbB2 pathways. In utero exposure to low dose BPA (500 ng/kg) induced mammary tumorigenesis, earlier puberty onset, increased terminal end buds, and prolonged estrus phase, which was accompanied by proliferative mammary morphogenesis. CD24/49f-based FACS analysis showed that in utero exposure to 500 ng/kg BPA induced expansion of luminal and basal/myoepithelial cell subpopulations at PND 35. Molecular analysis of mammary tissues at PND 70 showed that in utero exposure to low doses of BPA induced upregulation of ERα, p-ERα, cyclin D1, and c-myc, concurrent activation of erbB2, EGFR, erbB-3, Erk1/2, and Akt, and upregulation of growth factors/ligands. Our results demonstrate that in utero exposure to low dose BPA promotes mammary tumorigenesis in MMTV-erbB2 mice through induction of ER-erbB2 crosstalk and mammary epithelial reprogramming, which advance our understanding of the mechanism associated with in utero exposure to BPA-induced breast cancer risk. The studies also support using MMTV-erbB2 mouse model for relevant studies.
Degradation, Bone Regeneration and Tissue Response of an Innovative Volume Stable Magnesium-Supported GBR/GTR Barrier Membrane
Authors: Barbeck Mike , Kühnel Lennart , Witte Frank , Pissarek Jens , Precht Clarissa , Xiong Xin , Krastev Rumen , Wegner Nils , Walther Frank , Jung Ole
Published: April 2020

Introduction: Bioresorbable collagenous barrier membranes are used to prevent premature soft tissue ingrowth and to allow bone regeneration. For volume stable indications, only non-absorbable synthetic materials are available. This study investigates a new bioresorbable hydrofluoric acid (HF)-treated magnesium (Mg) mesh in a native collagen membrane for volume stable situations. Materials and Methods: HF-treated and untreated Mg were compared in direct and indirect cytocompatibility assays. In vivo, 18 New Zealand White Rabbits received each four 8 mm calvarial defects and were divided into four groups: (a) HF-treated Mg mesh/collagen membrane, (b) untreated Mg mesh/collagen membrane (c) collagen membrane and (d) sham operation. After 6, 12 and 18 weeks, Mg degradation and bone regeneration was measured using radiological and histological methods. Results: In vitro, HF-treated Mg showed higher cytocompatibility. Histopathologically, HF-Mg prevented gas cavities and was degraded by mononuclear cells via phagocytosis up to 12 weeks. Untreated Mg showed partially significant more gas cavities and a fibrous tissue reaction. Bone regeneration was not significantly different between all groups. Discussion and Conclusions: HF-Mg meshes embedded in native collagen membranes represent a volume stable and biocompatible alternative to the non-absorbable synthetic materials. HF-Mg shows less corrosion and is degraded by phagocytosis. However, the application of membranes did not result in higher bone regeneration.
The Deubiquitinating Enzyme USP20 Regulates the TNFα-Induced NF-κB Signaling Pathway through Stabilization of p62
Authors: Ha Jihoon , Kim Minbeom , Seo Dongyeob , Park Jin Seok , Lee Jaewon , Lee Jinjoo , Park Seok Hee
Published: April 2020

p62/sequestosome-1 is a scaffolding protein involved in diverse cellular processes such as autophagy, oxidative stress, cell survival and death. It has been identified to interact with atypical protein kinase Cs (aPKCs), linking these kinases to NF-κB activation by tumor necrosis factor α (TNFα). The diverse functions of p62 are regulated through post-translational modifications of several domains within p62. Among the enzymes that mediate these post-translational modifications, little is known about the deubiquitinating enzymes (DUBs) that remove ubiquitin chains from p62, compared to the E3 ligases involved in p62 ubiquitination. In this study, we first demonstrate a role of ubiquitin-specific protease USP20 in regulating p62 stability in TNFα-mediated NF-κB activation. USP20 specifically binds to p62 and acts as a positive regulator for NF-κB activation by TNFα through deubiquitinating lysine 48 (K48)-linked polyubiquitination, eventually contributing to cell survival. Furthermore, depletion of USP20 disrupts formation of the atypical PKCζ-RIPK1-p62 complex required for TNFα-mediated NF-κB activation and significantly increases the apoptosis induced by TNFα plus cycloheximide or TNFα plus TAK1 inhibitor. These findings strongly suggest that the USP20-p62 axis plays an essential role in NF-κB-mediated cell survival induced by the TNFα-atypical PKCζ signaling pathway.
Structural Analysis of Jumbo Coliphage phAPEC6
Authors: Wagemans Jeroen , Tsonos Jessica , Holtappels Dominique , Fortuna Kiandro , Hernalsteens Jean-Pierre , De Greve Henri , Estrozi Leandro F. , Bacia-Verloop Maria , Moriscot Christine , Noben Jean-Paul , Schoehn Guy , Lavigne Rob
Published: April 2020

The phAPEC6 genome encodes 551 predicted gene products, with the vast majority (83%) of unknown function. Of these, 62 have been identified as virion-associated proteins by mass spectrometry (ESI-MS/MS), including the major capsid protein (Gp225; present in 1620 copies), which shows a HK97 capsid protein-based fold. Cryo-electron microscopy experiments showed that the 350-kbp DNA molecule of Escherichia coli virus phAPEC6 is packaged in at least 15 concentric layers in the phage capsid. A capsid inner body rod is also present, measuring about 91 nm by 18 nm and oriented along the portal axis. In the phAPEC6 contractile tail, 25 hexameric stacked rings can be distinguished, built of the identified tail sheath protein (Gp277). Cryo-EM reconstruction reveals the base of the unique hairy fibers observed during an initial transmission electron microscopy (TEM) analysis. These very unusual filaments are ordered at three annular positions along the contractile sheath, as well as around the capsid, and may be involved in host interaction.
A Self-Operating Time Crystal Model of the Human Brain: Can We Replace Entire Brain Hardware with a 3D Fractal Architecture of Clocks Alone?
Authors: Singh Pushpendra , Saxena Komal , Singhania Anup , Sahoo Pathik , Ghosh Subrata , Chhajed Rutuja , Ray Kanad , Fujita Daisuke , Bandyopadhyay Anirban
Published: April 2020

Time crystal was conceived in the 1970s as an autonomous engine made of only clocks to explain the life-like features of a virus. Later, time crystal was extended to living cells like neurons. The brain controls most biological clocks that regenerate the living cells continuously. Most cognitive tasks and learning in the brain run by periodic clock-like oscillations. Can we integrate all cognitive tasks in terms of running clocks of the hardware? Since the existing concept of time crystal has only one clock with a singularity point, we generalize the basic idea of time crystal so that we could bond many clocks in a 3D architecture. Harvesting inside phase singularity is the key. Since clocks reset continuously in the brain–body system, during reset, other clocks take over. So, we insert clock architecture inside singularity resembling brain components bottom-up and top-down. Instead of one clock, the time crystal turns to a composite, so it is poly-time crystal. We used century-old research on brain rhythms to compile the first hardware-free pure clock reconstruction of the human brain. Similar to the global effort on connectome, a spatial reconstruction of the brain, we advocate a global effort for more intricate mapping of all brain clocks, to fill missing links with respect to the brain’s temporal map. Once made, reverse engineering the brain would remain a mere engineering challenge.
Range-Expansion in Processionary Moths and Biological Control
Authors: de Boer Jetske , Harvey Jeffrey
Published: April 2020

Global climate change is resulting in a wide range of biotic responses, including changes in diel activity and seasonal phenology patterns, range shifts polewards in each hemisphere and/or to higher elevations, and altered intensity and frequency of interactions between species in ecosystems. Oak (Thaumetopoea processionea) and pine (T. pityocampa) processionary moths (hereafter OPM and PPM, respectively) are thermophilic species that are native to central and southern Europe. The larvae of both species are gregarious and produce large silken ‘nests’ that they use to congregate when not feeding. During outbreaks, processionary caterpillars are capable of stripping foliage from their food plants (oak and pine trees), generating considerable economic damage. Moreover, the third to last instar caterpillars of both species produce copious hairs as a means of defence against natural enemies, including both vertebrate and invertebrate predators, and parasitoids. These hairs contain the toxin thaumetopoein that causes strong allergic reactions when it comes into contact with human skin or other membranes. In response to a warming climate, PPM is expanding its range northwards, while OPM outbreaks are increasing in frequency and intensity, particularly in northern Germany, the Netherlands, and southern U.K., where it was either absent or rare previously. Here, we discuss how warming and escape from co-evolved natural enemies has benefitted both species, and suggest possible strategies for biological control.
Synthesis of (R) and (S)-3-Chloro-5-(3-methylmorpholino)-4H-1,2,6-thiadiazin-4-ones
Authors: Kalogirou Andreas S. , Asquith Christopher R. M. , Koutentis Panayiotis A.
Published: April 2020

Reaction of 3,5-dichloro-4H-1,2,6-thiadiazin-4-one with (R) and (S)-3-methylmorpholines (2 equiv), in THF, at ca. 20 °C gave (R) and (S)-3-chloro-5-(3-methylmorpholino)-4H-1,2,6-thiadiazin-4-ones in 95 and 97% yields, respectively. The new compounds were fully characterized.
Enhancing Spectral Reflection through Controlled Phase Distribution Using Doped Polar-Dielectric Metasurfaces
Authors: Janipour Mohsen , Şendur Kürşat
Published: April 2020

Controlling the phase distribution of wavefronts using optical metasurfaces has led to interesting optical properties and applications. Here, we explore the control of phase distribution through polar-dielectric metasurfaces composed of doped SiC nanosphere arrays. We investigate the impact of doping concentration on the optical properties of SiC nano-spheres. Our results indicate that increasing the doping of SiC nanoparticles influenced electric dipolar resonances, whereas it did not change the dipolar resonances. Using this concept, we numerically studied the extension of this idea to form metasurface arrays of single, dimer and linear trimers of the doped SiC nano-spheres. Using different doping schemes, we studied the improvement of the reflectivity at frequencies greater than the longitudinal optical phonon frequency.
The Forced Magnetostrictions and Magnetic Properties of Ni2MnX (X = In, Sn) Ferromagnetic Heusler Alloys
Authors: Sakon Takuo , Yamazaki Junya , Komori Takumi , Kanomata Takeshi , Narumi Yasuo , Hagiwara Masayuki , Nojiri Hiroyuki , Adachi Yoshiya
Published: April 2020

Experimental studies into the forced magnetostriction, magnetization, and temperature dependence of permeability in Ni2MnIn and Ni2MnSn ferromagnetic Heusler alloys were performed according to the spin fluctuation theory of itinerant ferromagnetism proposed by Takahashi. We investigated the magnetic field (H) dependence of magnetization (M) at the Curie temperature TC, and at T = 4.2 K, which concerns the ground state of the ferromagnetic state. The M-H result at TC was analyzed by means of the H versus M5 dependence. At 4.2 K, it was investigated by means of an Arrott plot (H/M vs. M2) according to Takahashi’s theory. As for Ni2MnIn and Ni2MnSn, the spin fluctuation parameters in k-space (momentum space, TA) and that in energy space (frequency space, T0) obtained at TC and 4.2 K were almost the same. The average values obtained at TC and 4.2 K were TA = 342 K, T0 = 276 K for Ni2MnIn and TA = 447 K, T0 = 279 K for Ni2MnSn, respectively. The forced magnetostriction at TC was also investigated. The forced linear magnetostriction (ΔL/L) and the forced volume magnetostriction (ΔV/V) were proportional to M4, which followed Takahashi’s theory. We compared the forced volume magnetostriction ΔV/V and mechanical parameter, bulk modulus K. ΔV/V is inversely proportional to K. We also discuss the spin polarization of Ni2MnIn and other magnetic Heusler alloys. The pC/pS of Ni2MnIn was 0.860. This is comparable with that of Co2MnGa, which is a famous half-metallic alloy.
Influence of Applied Liquid-Type Scanning-Aid Material on the Accuracy of the Scanned Image: An In Vitro Experiment
Authors: Oh Hyun-Su , Lim Young-Jun , Kim Bongju , Kim Won Hyeon , Kim Myung-Joo , Kwon Ho-Beom
Published: April 2020

The study was designed to evaluate the effects of a liquid-type scanning-aid material on the accuracy and time efficiency of intraoral digital impressions compared to those of two different types of powder scanning-aid material and the powder-free scanning method. Three reference models (inlay, onlay, and bridge) were fabricated by a 3D printer and scanned with a model scanner to make the reference datasets. Four experimental groups (application of ScanCure, VITA, IP, and no treatment) were established, and the scans were acquired (each n = 5) using the Trios 3® (3 Shape, Copenhagen, Denmark). All scan data were digitally superimposed with the reference data (trueness, n = 5), and group comparisons were performed for each group (precision, n = 10). Time efficiency was evaluated by comparing the working times for scanning the models. The liquid-type ScanCure group showed fewer errors than the IP and VITA groups in all three reference models. Particularly, in the inlay model, the ScanCure group showed high accuracy compared to the powder-type groups (IP and VITA) with statistical significance (p < 0.001). The working time of the no-treatment group was longer than that of the agent groups in all reference models (p < 0.001). Notably, in the bridge model, the working time of the ScanCure group was shorter than that of the IP and VITA groups. Unlike other spray-type scanning-aid materials, this liquid-type material has the advantage of being thinly and uniformly applied to the object surface at the time of use. These findings suggest that the liquid-type scanning-aid material would be more accurate in achieving shape reproducibility using an intraoral scanner than the other two spray-type groups.
Analysis of the Extreme Equilibrium Conditions of an Internal Cavity Located Inside a Flat Metal Plate Subjected to an Internal Pressure p
Authors: Tamayo-Meza Pedro Alejandro , Silva-Rivera Usiel Sandino , Flores-Herrera Luis Armando , Rodríguez-Alvarado Lisaura Walkiria , Pérez-Cruz José Humberto , Rivera-López Jesús Eduardo
Published: April 2020

The influence of surface bulges and cavities within metals is an important metallurgical-mechanical problem that has not been fully solved and motivates multiple discussions. This is not only related to the generation of interfaces, but also to the distribution of alloying components and elements. In this study, Laplace’s equation was used to develop a set of equations to describe these kinds of defects in plates, which arise during the development of metallurgical processes, and this can be used for the prediction of pipeline failures subjected to internal pressure. In addition, the stability conditions of a cavity under an internal pressure are analyzed. The developed method allows to identify the stress state in the generation of the cavity and its propagation. In addition to this, finite element analyses were carried out in order to show first the stress distribution around a cavity subjected to a series of theoretical operation conditions and second to show the crack growth on the tip of the cavity.
NiYAl-Derived Nanoporous Catalysts for Dry Reforming of Methane
Authors: Imada Syota , Peng Xiaobo , Cai Zexing , Najib Abdillah Sani Bin Mohd , Miyauchi Masahiro , Abe Hideki , Fujita Takeshi
Published: April 2020

Dry reforming of methane can be used for suppressing the rapid growth of greenhouse gas emissions. However, its practical implementation generally requires high temperatures. In this study, we report an optimal catalyst for low-temperature dry reforming of methane with high carbon coking resistance synthesized from NiYAl alloy. A facile two-step process consisting of preferential oxidation and leaching was utilized to produce structurally robust nanoporous Ni metal and Y oxides from NiYAl4. The catalyst exhibited an optimal carbon balance (0.96) close to the ideal value of 1.0, indicating the optimized dry reforming pathway. This work proposes a facile route of the structural control of active metal/oxide sites for realizing highly active catalysts with long-term durability.
Proposed Constitutive Law of Uniaxial Compression for Concrete under Deterioration Effects
Authors: Gao Yiwei , Ren Xuhua , Zhang Jixun , Zhong Lingwei , Yu Shuyang , Yang Xiang , Zhang Wenbing
Published: April 2020

In order to study the ductile deformation characteristics and failure process of plain concrete under uniaxial compression, this paper proposes a new constitutive model. The new model was used to fit and analyze the constitutive curve of concrete under uniaxial compressive under various degradation forms and was compared with the traditional constitutive models. Finally, the new model was used to quantitatively analyze and predict the stress–strain curve of concrete in different degradation periods of a set of freeze–thaw measured data. The results show that, compared with the traditional constitutive model, the new model is simple in form and has few parameters, and the numerical value of the parameter can reflect the ductile deformation capacity of concrete. The fitting curve of the new model has the highest fitting degree with the measured stress–strain curve of concrete, and the goodness of fit (R2) is also the largest. The new model is suitable for fitting the stress–strain curve of concrete under uniaxial compression under various deteriorating forms, and the degree of fit between the constitutive prediction curve and the measured curve is high. It can be seen from the fitting results of the new model parameters that the ductile deformation capacity of concrete decreases first and then increases slightly, which is inconsistent with the law of gradual deterioration of strength. There is a minimum moment of ductility deformation capacity of concrete (MDC). The MDC of O-C40 concrete is about 114 freeze–thaw cycles, and the MDC of O-C50 concrete is about 116 freeze–thaw cycles; the degree of fit between the constitutive prediction curve and the measured curve is high. We hope that the improvement mentioned offers valid reference to the study of ductile deformation characteristics and failure process of compressed concrete under different deterioration forms.
Potential of Stainless Steel Slag Waste in Manufacturing Self-Compacting Concrete
Authors: Rosales Julia , Agrela Francisco , Entrenas José Antonio , Cabrera Manuel
Published: April 2020

The volume of slags generated from the steel industry is a source of possible resources which is constantly increasing. Specifically, in the production of stainless steel, specific and singular slags with unique characteristics are obtained, which allows considering an approach aimed at their use in new recycling ways. This work shows the feasibility of using stainless steel slag as a substitute for limestone filler in the manufacture of self-compacting concrete. The influence of different treatments applied to slags on physical and chemical properties was studied. On the other hand, the mechanical behaviour, as well as the durability acquired in self-compacting concrete, has been analysed. Very encouraging results were obtained, since this research demonstrates the possible application of this stainless steel slag as a construction material, improving sustainability and promoting circular economy processes, which are achieved through the minimisation of the waste disposal and accumulation.
FEM-Based Wave Propagation Modelling for SHM: Certain Numerical Issues in 1D Structures
Authors: Palacz Magdalena , Żak Arkadiusz , Krawczuk Marek
Published: April 2020

The numerical modelling of structural elements is an important aspect of modern diagnostic systems. However, the process of numerical implementation requires advanced levels of consideration of multiple aspects. Important issues of that process are the positive and negative aspects of the methods applied. Therefore the aim of this article is to familiarise the reader with the most important aspects related to the process of numerical modelling of one-dimensional problems related to the phenomena of the propagation of elastic waves and their application for damage detection purposes.
Effect of Substrate Grain Size on Structural and Corrosion Properties of Electrodeposited Nickel Layer Protected with Self-Assembled Film of Stearic Acid
Authors: Noorbakhsh Nezhad Amir Hossein , Rahimi Ehsan , Arefinia Reza , Davoodi Ali , Hosseinpour Saman
Published: April 2020

In the present study, the impact of copper substrate grain size on the structure of the succeeding electrodeposited nickel film and its consequent corrosion resistance in 3.5% NaCl medium were evaluated before and after functionalization with stearic acid. Nickel layers were electrodeposited on two different copper sheets with average grain size of 12 and 25 µm, followed by deposition of stearic acid film through self-assembly. X-ray diffraction analysis of the electrodeposited nickel films revealed that the deposition of nickel film on the Cu substrate with small (12 µm) and large (25 µm) grains is predominantly governed by growth in the (220) and (111) planes, respectively. Both electrodeposited films initially exhibited a hydrophilic nature, with water-contact angles of 56° and <10°, respectively. After functionalization with stearic acid, superhydrophobic films with contact angles of ~150° were obtained on both samples. In a 3.5% NaCl medium, the corrosion resistance of the nickel layer electrodeposited on the copper substrate with 25 µm grains was three times greater than that deposited on the copper substrate with 12 µm grains. After functionalization, the corrosion resistance of both films was greatly improved in both short and long immersion times in 3.5% NaCl medium.
Effect of Sulfur Content on the Composition of Inclusions and MnS Precipitation Behavior in Bearing Steel
Authors: Zhang Hongliang , Feng Guanghong , Liu Xin , Wang Baoshan , Liu Xuming
Published: April 2020

MnS inclusions in bearing steel have long been considered to significantly affect the fatigue life of bearing steel. In this paper, the sizes of inclusions in bearing steel with different sulfur contents were analyzed and the precipitation behavior of MnS was calculated using thermodynamics. Furthermore, the positive role of MnS in bearing steel was discussed. Results showed that when the size of inclusions in bearing steel was increased, the proportion of MnS components in composite inclusions gradually decreased. When the sulfur content was increased, the shape of inclusions changed from a particle shape to a strip shape. With increasing MnS content, the inclusion ratio of Al2O3 was significantly reduced in the Al2O3–CaO–MgO–MnS quaternary inclusion system, particularly for MnS proportions greater than 20%. The content of sulfur in bearing steel significantly affects the precipitation temperature of MnS. When sulfur content increases from 0.001% to 0.007%, the precipitation temperature of MnS increases from 1493 K to 1633 K as the precipitation of MnS moves from the austenite solid phase to the liquid and solid phases, and the precipitation size of MnS inclusions significantly increases. The size of oxide inclusions should be controlled to improve MnS wrap oxide inclusions in steel. Based on these results, a composition control with high sulfur levels and low oxygen levels should be adopted to improve the fatigue performance of steel.
Recovery of Gold from the Refractory Gold Concentrate Using Microwave Assisted Leaching
Authors: Cho Kanghee , Kim Hyunsoo , Myung Eunji , Purev Oyunbileg , Choi Nagchoul , Park Cheonyoung
Published: April 2020

Microwave technology has been confirmed to be suitable for use in a wide range of mineral leaching processes. Compared to conventional leaching, microwave-assisted leaching has significant advantages. It is a proven process, because of its short processing time and reduced energy. The purpose of this study was to enhance the gold content in a refractory gold concentrate using microwave-assisted leaching. The leaching efficiencies of metal ions (As, Cu, Zn, Fe, and Pb) and recovery of gold from refractory gold concentrate were investigated via nitric acid leaching followed by microwave treatment. As the acid concentration increased, metal ion leaching increased. In the refractory gold concentrate leaching experiments, nitric acid leaching at high temperatures could limit the decomposition of sulfide minerals, because of the passive layer in the refractory gold concentrate. Microwave-assisted leaching experiments for gold recovery were conducted for the refractory gold concentrate. More extreme reaction conditions (nitric acid concentration > 1.0 M) facilitated the decomposition of passivation species derived from metal ion dissolution and the liberation of gangue minerals on the sulfide surface. The recovery rate of gold in the leach residue was improved with microwave-assisted leaching, with a gold recovery of ~132.55 g/t after 20 min of the leaching experiment (2.0 M nitric acid), according to fire assays.
A Review of Passive Micromixers with a Comparative Analysis
Authors: Raza Wasim , Hossain Shakhawat , Kim Kwang-Yong
Published: April 2020

A wide range of existing passive micromixers are reviewed, and quantitative analyses of ten typical passive micromixers were performed to compare their mixing indices, pressure drops, and mixing costs under the same axial length and flow conditions across a wide Reynolds number range of 0.01–120. The tested micromixers were selected from five types of micromixer designs. The analyses of flow and mixing were performed using continuity, Navier-Stokes and convection-diffusion equations. The results of the comparative analysis were presented for three different Reynolds number ranges: low-Re (Re ≤ 1), intermediate-Re (1 < Re ≤ 40), and high-Re (Re > 40) ranges, where the mixing mechanisms are different. The results show a two-dimensional micromixer of Tesla structure is recommended in the intermediate- and high-Re ranges, while two three-dimensional micromixers with two layers are recommended in the low-Re range due to their excellent mixing performance.
MEMS Mirrors for LiDAR: A Review
Authors: Wang Dingkang , Watkins Connor , Xie Huikai
Published: April 2020

In recent years, Light Detection and Ranging (LiDAR) has been drawing extensive attention both in academia and industry because of the increasing demand for autonomous vehicles. LiDAR is believed to be the crucial sensor for autonomous driving and flying, as it can provide high-density point clouds with accurate three-dimensional information. This review presents an extensive overview of Microelectronechanical Systems (MEMS) scanning mirrors specifically for applications in LiDAR systems. MEMS mirror-based laser scanners have unrivalled advantages in terms of size, speed and cost over other types of laser scanners, making them ideal for LiDAR in a wide range of applications. A figure of merit (FoM) is defined for MEMS mirrors in LiDAR scanners in terms of aperture size, field of view (FoV) and resonant frequency. Various MEMS mirrors based on different actuation mechanisms are compared using the FoM. Finally, a preliminary assessment of off-the-shelf MEMS scanned LiDAR systems is given.
Legume Consumption Patterns in US Adults: National Health and Nutrition Examination Survey (NHANES) 2011–2014 and Beans, Lentils, Peas (BLP) 2017 Survey
Authors: Perera Thushanthi , Russo Candace , Takata Yumie , Bobe Gerd
Published: April 2020

Given the emerging health benefits of regular legume consumption, we hypothesized that the historically low legume consumption levels in US adults increased. We evaluated legume consumption patterns in US adults using cross-sectional data from the 2011–2012 and 2013–2014-year cycles of National Health and Nutrition Examination Surveys (NHANES) and a 2017 cross-sectional, online survey of Oregon families named “Beans, Lentils, Peas (BLP) Survey”. We also compared legume consumption patterns between consumers below US dietary recommendations for mature legumes (<37.5 g/day, marginal), below levels showing nutritional and disease-prevention benefits (37.5–87.49 g/day, recommended); and levels demonstrating nutritional and disease prevention benefits (≥87.5 g/day; beneficial). In NHANES, legume consumption remained low in US adults and declined from 2011 to 2014 (mature legumes: 12.8 to 8.3%; dry beans: 10.0 to 6.5%). In BLP, less than 5% consumed legumes daily; approximately one-third did not consume legumes during the last month. Marginal mature-legume consumers ate a limited variety of legumes (dry beans and green legumes on a weekly to monthly basis). Beneficial amount consumers ate mature legumes daily or every other day and included chickpeas, lentils and dry peas to their legume mix. Our data suggest that legume consumption declined in US adults, warranting improved communication about the benefits of regular legume consumption.
Impact of Stress Levels on Eating Behaviors among College Students
Authors: Choi Jinkyung
Published: April 2020

Weight gain is a common phenomenon among college students, especially those in their first year of university. Transitioning from high school to the college environment might increase perceived stress levels, thus affecting dietary behaviors and metabolism to promote overweight and obesity. The purpose of this study was to investigate the physical activities and dietary behaviors of college students in the context of their perceived stress levels. In addition, the demographic characteristics of the students were compared to ascertain their impact on dietary behaviors. Self-reported questionnaires were distributed to college students on campus in Korea. Perceived stress was measured by the 10-item Perceived Stress Scale (PSS-10), the scores for which were evaluated by independent t-tests to compare the dietary behaviors of the high- and low-perceived stress groups. Exploratory factor analysis was performed and Cronbach’s alphas were computed to assess the validity and internal consistency of the PSS-10 measurement items. Differences in the physical activities and dietary behaviors of the college students based on demographics such as sex, academic year, and residence type were found. Several dietary behaviors were significantly different between students with low and high perceived stress levels. Students with high perceived stress levels exhibited increased unhealthy dietary behaviors such as ready-prepared meal consumption (p < 0.001). These results suggest that stress management should be offered to college students. In addition, programs should be provided to help first-year students adjust to the college environment in order to promote healthy dietary behaviors.
Exposure to the Danish Mandatory Vitamin D Fortification Policy in Prenatal Life and the Risk of Developing Coeliac Disease—The Importance of Season: A Semi Ecological Study
Authors: Moos Caroline , Duus Katrine S. , Frederiksen Peder , Heitmann Berit L. , Andersen Vibeke
Published: April 2020

Few studies have examined the role of maternal diet in relation to development of coeliac disease (CD). In Denmark, cancellation of mandatory vitamin D fortification of margarine in June 1985 provided this opportunity. This study examined if season of birth or prenatal exposure to extra vitamin D from food fortification were associated with developing CD later in life. A strength of this study is the distinctly longer follow-up of patients (30 years). This register-based study has a semi-ecological design. Logistic regression analysis was used to estimate odds ratios and to calculate 95% confidence intervals. The odds ratio for developing CD was 0.81 (95% CI 0.66; 1.00 p = 0.054), comparing those with fetal exposure to mandatory vitamin D fortification policy of margarine to those without after adjusting for gender and season of birth. There was a statistically significant season effect particularly for children born in autumn (OR 1.6 95% CI 1.16; 2.21) and born in summer (OR 1.5 95% CI 1.1; 2.1) when compared to children born in winter. Although this study did not find evidence to support the premise that prenatal exposure to small extra amounts of vitamin D from a mandatory food fortification policy lowered risk of developing CD, the small number of CD cases and observed association between season of birth and CD suggest that environmental exposure ought to be further explored.
The Role of CT-Quantified Body Composition on Longitudinal Health-Related Quality of Life in Colorectal Cancer Patients: The Colocare Study
Authors: Gigic Biljana , Nattenmüller Johanna , Schneider Martin , Kulu Yakup , Syrjala Karen L. , Böhm Jürgen , Schrotz-King Petra , Brenner Hermann , Colditz Graham A. , Figueiredo Jane C. , Grady William M. , Li Christopher I. , Shibata David , Siegel Erin M. , Toriola Adetunji T. , Kauczor Hans-Ulrich , Ulrich Alexis , Ulrich Cornelia M.
Published: April 2020

Background: Obesity, defined by body mass index (BMI), measured at colorectal cancer (CRC) diagnosis has been associated with postoperative complications and survival outcomes. However, BMI does not allow for a differentiation between fat and muscle mass. Computed tomography (CT)-defined body composition more accurately reflects different types of tissue and their associations with health-related quality of life (HRQoL) during the first year of disease, but this has not been investigated yet. We studied the role of visceral and subcutaneous fat area (VFA and SFA) and skeletal muscle mass (SMM) on longitudinally assessed HRQoL in CRC patients. Methods: A total of 138 newly diagnosed CRC patients underwent CT scans at diagnosis and completed questionnaires prior to and six and twelve months post-surgery. We investigated the associations of VFA, SFA, and SMM with HRQoL at multiple time points. Results: A higher VFA was associated with increased pain six and twelve months post-surgery (β = 0.06, p = 0.04 and β = 0.07, p = 0.01) and with worse social functioning six months post-surgery (β = −0.08, p = 0.01). Higher SMM was associated with increased pain twelve months post-surgery (β = 1.03, p < 0.01). Conclusions: CT-quantified body composition is associated with HRQoL scales post-surgery. Intervention strategies targeting a reduction in VFA and maintaining SMM might improve HRQoL in CRC patients during the first year post-surgery.
Cardiorespiratory Fitness, Physical Activity, Sedentary Time and Its Association with the Atherogenic Index of Plasma in Chilean Adults: Influence of the Waist Circumference to Height Ratio
Authors: Reyes-Ferrada Waleska , Solis-Urra Patricio , Plaza-Díaz Julio , Sadarangani Kabir P. , de Moraes Ferrari Gerson Luis , Rodríguez-Rodríguez Fernando , Cristi-Montero Carlos
Published: April 2020

Atherogenic index of plasma (AIP) is a novel biomarker related to cardiovascular disease (CVD). Cardiorespiratory fitness (CRF) and physical activity (PA) have an inverse relationship with the AIP, while sedentary time (ST) and fatness present a positive association. This study aimed to determine the combined and independent association of CRF, PA, and ST with the AIP, and additionally to establish the waist-to-height ratio (WHtR) mediation role. Data from the Chilean national health survey were used (4671 adults). A PACS (Physical Activity Cardiorespiratory Sedentary) score was created ranging from 0 to 3, indicating the number of positive recommendations met (PA, ST, and CRF). AIP was calculated (Log10 triglycerides/high-density lipoprotein-cholesterol). The combined analysis showed that compared to those with a PACS score of 0, those with a score of 1 or 2 did not present significantly reduced AIP values (adjusted by the WHtR); however, those with a score of 3 did (OR (odds ratio) = 0.50; 95% CI, 0.32 to 0.77; p < 0.001). Independent analysis showed that CRF seems to be the only variable that supports the combined result (β = −0.212; p < 0.001). Finally, the mediation analysis indicated that the WHtR mediated the association between CRF and the AIP in 34.2% of cases. Overall, only CRF had a significant and inverse association with the AIP. Nonetheless, around one-third of this beneficial relationship is affected by an elevated WHtR.