bluuebunny/crossref_metadata_2025 huggingface dataset:
Neurogenesis-independent mechanisms of MRI-detectable hippocampal volume increase following electroconvulsive stimulation
Authors: Takamiya Akihiro , Abe Yoshifumi , Yokoyama Kiichi , Kato Tomonobu , Yagishita Sho , Tanaka Kenji
Published: August 2023

Electroconvulsive therapy (ECT) is the most effective psychiatric treatment but the underlying mechanisms are still unclear. In vivo human magnetic resonance imaging (MRI) studies have consistently reported ECT-induced transient hippocampal volume increases, and an animal model of ECT (electroconvulsive stimulation: ECS) was shown to increase neurogenesis. However, a causal relationship between neurogenesis and MRI-detectable hippocampal volume increases following ECT has not been verified. In this study, we were randomly allocated into four groups, each undergoing a different number of ECS sessions (e.g., 0, 3, 6, 9). T2-weighted image was acquired from those mice using 11.7-tesla MRI. A whole brain voxel-based morphometry analysis was conducted to identify any ECS-induced brain volume changes. Additionally, histological examinations with super-resolution microscopy were conducted to investigate microstructural changes in brain regions which show volume changes following ECS. Furthermore, parallel experiments were performed on X-ray irradiated mice to investigate a causal relationship between neurogenesis and ECS-related volume changes. As a result, we revealed for the first time that ECS induced MRI-detectable dose-dependent hippocampal volume increase in mice. Furthermore, ECS increased hippocampal volumes even in mice lacking neurogenesis, suggesting that neurogenesis is not required for the increase. Comprehensive histological analyses identified an increase in excitatory synaptic density in the ventral CA1 as a major contributor to the observed hippocampal volume increase following ECS. Our findings demonstrate that modification of synaptic structures rather than neurogenesis may be the underlying biological mechanism of ECT/ECS-induced hippocampal volume increase.
Biosensor –Based vital signs data aggregation and analytics for monitoring of patients in Internet of Medical Things (IoMT) enabled e-healthcare platform
Authors: Eteng Idongesit E. , Agana Moses A. , EKORO EKORO
Published: August 2023

Lack of proper patient health monitoring and data aggregation scheme in healthcare facilities has become an issue of serious concern the world over. Biosensors have become a handy way for the transmission of data from patients to healthcare providers. The use of Biosensors is also an innovation in healthcare management which involves observing vital physiological signs of patients with the aim of predicting ailments to mitigate them and prevent acute complications. In this study, a system architecture for proper monitoring of patients’ health using Internet of Medical things (IoMT) as an approach is developed. The Design Science research methodology was adopted in this research. The methodology involves the construction and evaluation of the artefacts that address a significantly recognized problem. In the adopted approach, real time data are sent to a local server via communication channels (Wi-Fi technology) and then transmitted to the IoT server via a designated network using a Wi-Fi module. For a robust transmission of physiological parameters to the cloud, a Blynk IoT- server was used as platform. Two main sensors DS18B20 an AD8232 ECG were used in measuring body temperature and heart rate respectively, with XAMPP server as the local server platform. Our results include a developed prototype and a mechanism where real-time numerical data is transmitted to the report platform which is later transformed into ordered numbers. The innovation in this work involves reading real time data from sensors attached to patients in IoMT-enabled environments and using a simple linear regression model to convert these real time data to ordered numbers which indicate severity of the physiological signals. These ordered numbers are transformed to visuals to enable ease of view. To the best of our knowledge, this approach has not been implemented by previous research studies. Based on our findings, we recommend that: the prototype system be deployed in healthcare facilities and the designed web applications be plugged into existing web domains of hospitals to enable timely intervention by the medical experts.
A Novel Feature Selection Approach Based Sampling Theory on Grapevine Images using Convolutional Neural Networks
Authors: Özaltın Öznur , Koyuncu Nursel
Published: August 2023

Feature selection, reducing number of input variables to develop classification model, is an important process to reduce computational and modelling complexity and affects the performance of image process. In this paper, we have proposed new statistical approaches for feature selection based on sample selection. We have applied our new approaches to grapevine leaves data that possesses properties of shape, thickness, featheriness, and slickness are investigated in images. To analyze such kind of data by using image process, thousands of features are created and selection of features plays important role to predict the outcome properly. In our numerical study, Convolutional Neural Networks (CNNs) have been used as feature extractors and then obtained features from the last average pooling layer to detect the type of grapevine leaves from images. These features have been reduced by using our suggested four statistical methods: Simple random sampling (SRS), ranked set sampling (RSS), extreme ranked set sampling (ERSS), Moving extreme ranked set sampling (MERSS). Then selected features have been classified with Artificial Neural Network (ANN) and we have obtained the best accuracy of 97.33% with our proposed approaches. Based on our empirical analysis, it has been determined that the proposed approach exhibits efficacy in the classification of grapevine leaf types. Furthermore, it possesses the potential for integration into various computational devices.
Impact of mutational status on preoperative olfactory function in olfactory groove meningioma: Insights from a Clinical Study
Authors: Podlesek Dino , Beyer Friederike , Alkhatib Majd , Hijazi Max Mido , Menzel Susanne , Eyüpoglu Ilker Yasin , Schackert Gabriele , Juratli Tareq Adnan , Hummel Thomas
Published: August 2023

PURPOSE:
The study aimed to analyze the impact of driver mutations and image characteristics of olfactory groove meningioma (OGM) on olfactory function.
METHODS:
Olfactory function was assessed in 22 patients with OGM using odor threshold, odor discrimination, odor identification, and a global olfactory score. Targeted sequencing was performed to define the most frequent OGM driver mutations in 52 patients. Image morphology was obtained from preoperative imaging.
RESULTS:
The cohort of 52 patients exhibited SMO/SUFU mutations in 17 (33%), AKT1 mutations in 13 (25%), and "other" mutations in 15 (29%) patients, while the remaining 7 (13%) tumors were wild-type. Tumor-associated planum hyperostosis was displayed in 75% of OGM, with the lowest frequency in the AKT1-mutated group (42%), the highest in the SMO/SUFU (82%) and WT-groups (100%); (p = .017). Related to OGM volume, there was a difference between AKT1 and wild-type mutated OGM cohort ([Median (Range) cm3]: [13 (3-45) cm3] vs. [44 (19-84) cm3]) (p = .008). The preoperative odor threshold correlated with the perifocal edema (p = .03). Odor identification was associated with tumor volume (p = .014) and hyperostosis of the planum (p = .005).
CONCLUSIONS:
The study provides a reference on the dependencies between imaging, clinical, and OGM mutational characteristics and preoperative olfactory performance. For the first time, it has been shown that PIK3CA, TRAF7-, POLR2A-, and NF1 OGM-mutation status is a tumor-related molecular predictor of the preoperative global olfactory performance. The preoperative olfactory status could contribute to more accurate surgical planning and postoperative patient support.
Dynamics of a fragmented forest landscape and its drivers of forest loss, recovery, and stability
Authors: Cunha Jéssica Stéfane Alves , Santos Jhonathan Gomes , Fonsêca Nathan Castro , Rezende Camila Linhares , Lins-e-Silva Ana Carolina Borges
Published: August 2023

Given the current demands for biodiversity conservation and climate mitigation, understanding the dynamics of fragmented forests in one of the most threatened regions of the Atlantic Forest is essential for supporting small and large-scale forest restoration initiatives. We investigated the patterns of forest loss, recovery, and stability from 1985 to 2020. We classified and quantified forest cover using temporal and spatial descriptors and related them to their causal agents. We hypothesised that there is a spontaneous natural regeneration process in abandoned areas, protected areas, and near the hydrographic network, but anthropogenic land uses negatively affect this process. Consequently, we observed that despite an increase in forest cover over the years, there was a loss of medium and large fragments of old-growth forests due to agriculture and urban infrastructure expansion, especially in flat areas and near watercourses. The increase was mostly comprised of small young forests in abandoned and protected areas, especially near watercourses. Forest stability was explained by proximity to municipality administrative units. This research elucidates the dynamics of forest patches in a severely threatened Atlantic Forest region. It revealed the replacement of large and medium mature forest fragments with small fragments of young forest. Thus, our results reinforce the need for monitoring forest stability, avoiding forest loss, and incentivising forest gain for biodiversity and ecosystem service conservation.
Exploring the Potential of a Meditation-Based Liver Cancer Classification Signature for Adjunctive Therapy
Authors: Zhou Xinyi , Zhang Zhimeng , Lan Fanglin , Bai Weiyu
Published: August 2023

Mindfulness meditation, as a non-therapeutic approach, holds immense significance in alleviating stress and regulating mental well-being. However, its utilization in disease treatment and clinical contexts remains largely unexplored. In light of cutting-edge research involving RNA sequencing data, we have successfully developed a gene signature, derived from differential gene expression during four distinct stages of meditation, to effectively classify patients with liver cancer. Our findings indicate that liver cancer patients can be stratified into two subtypes characterized by varying prognoses, immune microenvironments, tumor mutation burdens, and drug sensitivities. This tailored classification approach not only enables personalized chemotherapy strategies for liver cancer patients but also integrates meditation as a complementary intervention, significantly mitigating the adverse effects associated with chemotherapy.
Inhibition of cisplatin-induced Acsl4-mediated ferroptosis alleviated ovarian injury
Authors: Wang Siyuan , Li Xuqing , Li Jun , Wang Aiping , Li Fangfang , Hu Huiqing , Long Tengfei , Pei Xueting , Li Hongyan , Zhong Fei , Zhu Fengyu
Published: August 2023

Given that the severity of the chemotherapy-induced ovarian damage, effective fertility preservation is a necessary part of the treatment process. Ferroptosis is a regulated cell death triggered by excessive phospholipid peroxidation caused by iron and the role of ferroptosis in chemotherapy-induced ovarian damage remains unclear. In this study, we demonstrated that cisplatin treatment caused the accumulation of iron ions which induced ferroptosis in ovarian tissue. And our results show that ferrostatin-1 was able to suppress the ovarian injury and granulosa cell death caused by cisplatin (Cis) in vivoand in vitro. At the same time, we observed significant changes in the expression levels of Acyl-CoA synthetase long-chain family member 4 (Acsl4) and glutathione peroxidase 4 (GPX4). Similarly, Rosiglitazone, an inhibitor of Acsl4, administration alleviated the ovary damage of the mice undergoing chemotherapy. Further mechanistic investigation showed that cisplatin increased the expression level of specificity protein 1 (SP1), and SP1 could bind to the promoter of Acsl4 to increased Acsl4 transcription. Overall, ferroptosis plays an important role in Cis induced ovarian injury, and inhibition of ferroptosis protects ovarian tissues from damage caused by cisplatin, and for the first time, we have identified the potential of Fer-1 and Rosi to protect ovarian function in female mice undergoing chemotherapy.
Photo-induced anomalous Hall effect in ferromagnetic (Co)/transition metal dichalcogenide (WS 2 ) heterostructure
Authors: Jannati Saeid , Aftabi Ali , Rafiei Ali , Tehranchi Mohammad Mehdi
Published: August 2023

The photo-induced Hall effect is shown in the Co/WS2 heterostructure. This heterostructure consists of a ferromagnetic metal layer that forms a Schottky junction to a transition metal dichalcogenide semiconductor layer. In this effect, under the magnetic field and light irradiation, the excited charge carriers from the WS2 layer diffuse into the Co layer and are driven by Lorentz forces, then creates a transverse voltage in the open circuit. This open circuit voltage is a function of light and magnetic field intensity. The photo-induced voltage is nonlinear and hysteretic with the applied magnetic field and is in good agreement with magneto-optical Kerr's hysteresis loops. The observed results can provide a new technique for optical and magnetic sensing applications. In addition, it proposed a new technique to reconstruct the in-plane magnetic hysteresis loop for metallic thin films.
Research on the targeted improvement of the yield of a new VB12-producing strain, Ensifer adhaerens S305, based on genome and transcriptome analysis
Authors: Yongheng Yongheng Liu , Wei Wei Huang , Qi Qi Wang , Cilang Cilang Ma , Yongyong Yongyong Chang , Jianyu Jianyu Su
Published: August 2023

Background
Vitamin B12 (VB12) has wide application and high economic value. In this study, a new strain with high VB12 production potential, Ensifer adhaerens S305, was identified in sewage. Because E. adhaerens strains have become the main strains for VB12 production via fermentation in recent years, the directional modification of the S305 strain towards a strain suitable for the industrial production of VB12 has great potential and commercial value.
Results
16S rRNA and genome-wide phylogenetic tree analysis combined with average nucleotide identity (ANI) value analysis showed that the high-yielding VB12 strain belongs to E. adhaerens and that its VB12 synthesis pathway genes are highly similar to related genes of strains of this species, including E. adhaerens Casida A, Pseudomonas denitrificans SC 510, and E. adhaerens Corn53. High-pressure liquid chromatography (HPLC) results indicated that the VB12 yields of the S305 strain were more than double those of the Casida A strain under different medium components. Multiple genes with significantly upregulated and downregulated transcription were identified by comparing the transcription intensity of different genes through transcriptome sequencing. KEGG enrichment analysis of the porphyrin metabolism pathway identified 9 significantly upregulated and downregulated differentially expressed genes (DEGs) in the VB12 synthesis pathway, including 7 transcriptionally upregulated genes (cobA, cobT, hemA, cobJ, cobN, cobR, and cobP) that were episomally overexpressed in the Casida A strain. The results showed that the VB12 yield of the overexpressed strain was higher than that of the wild strain. Notably, the strains overexpressing the cobA and cobT genes had the most significant increases in VB12 yield, i.e., 31.4% and 24.8%, respectively. The VB12 yield of the S305 strain in shake-flask culture improved from 176.6 ± 8.21 mg/L to 245.6 ± 4.36 mg/L by integrating the cobA and cobT genes into the S305 strain.
Conclusion
Phylogenetic tree and ANI analysis showed that the Ensifer and Sinorhizobium strains are quite different at the genome level; the overexpression and integrated expression of significantly upregulated genes in the VB12 synthesis pathway can increase the yield of VB12, further improving the VB12 yield of the E. adhaerens S305 strain.
Urocortin-1 Promotes Colorectal Cancer Cells Migration, Proliferation and Inhibits Apoptosis via Inhibition of p53 Signaling Pathway
Authors: Guo Xiaolan , Li Ya , Chen Xiangyu , Sun Binghua
Published: August 2023

Purpose
To investigate the effect of urocortin-1 (UCN-1) on the growth, migration and apoptosis of colorectal cancer (CRC) in vivo and vitro and mechanism of UCN-1 modulating CRC cells in vitro.
Methods
The correlation between UCN-1 and CRC was evaluated by Cancer Genome Atlas (TCGA) database and the tissues microarray. The expression of UCN-1 in CRC cells was explored by quantitative real-time polymerase chain reaction (RT-qPCR) or western blot. In vitro, the influence of UCN-1 on proliferation, apoptosis and migration HCT-116 and RKO cells were explored by celigo cell counting assay, flow cytometry and wound healing assay or transwell, respectively. In vivo the effect of UCN-1 on CRC tumor growth and progression was evaluated in the nude mice. The downstream pathway behind UCN-1 regulating CRC was found by phospho-kinase profiler array in RKO cells. Expression of UCN-1 in cells was knocked down or upregulated using lentivirus.
Results
Both of the results of TCGA database and the tissues microarray shown that UCN-1 strongly expressed in tissues of CRC patients. Furthermore, the tissues microarray results showed that expression of UCN-1 was higher in male CRC patients than that in female patients, and high expression of UCN-1 was associated with higher risk of lymphatic metastasis and later pathological stage. Additionally, knockdown of UCN-1 in CRC cells caused a reduction in cell proliferation, migration, and colony formation as well as an increase in apoptosis. In xenograft experiments, tumors generated from RKO cells with UCN-1 knockdown exhibited declined tumor volume and weight. Reduction of the expression of Ki67 in xenograft tumors reflected that knockdown of UCN-1 curbed the growth of CRC tumors. Furthermore, the human phospho-kinase array showed that p53 signal pathway participated in UCN-1-mediated CRC development. The suppression in migration and proliferation caused by UCN-1 knockdown was reversed by inhibitors of p53 signal pathway, while the increase of cell apoptosis was withdrawn. On the other hand, overexpression of UCN-1 promoted the proliferation and migration and inhibited apoptosis of CRC cells. Overexpression of p53 reversed the effect of UCN-1 overexpression on CRC development.
Conclusion
UCN-1 promotes the migration, proliferation and inhibits apoptosis via inhibition of p53 signaling pathways.
Differentiation between depression and bipolar disorder in child and adolescents by voice features
Authors: luo jie , wu yuanzhen , Liu Mengqi , Li Zhaojun , Wang Zhuo , zheng yi , Feng Lihui , lu jihua , he fan
Published: August 2023

Objective: Major depressive disorder (MDD) and bipolar disorder (BD) are serious chronic disabling mental and emotional disorders, with symptoms that often manifest atypically in children and adolescents, making diagnosis difficult without objective physiological indicators. Therefore, we aimed to objectively identify MDD and BD in children and adolescents by exploring their voiceprint features.
Methods:This study included a total of 150 participants, with 50 MDD patients, 50 BD patients, and 50 healthy controls aged between 6 to 16 years. After collecting voiceprint data, chi-square test was used to screen and extract voiceprint features specific to emotional disorders in children and adolescents. Then, selected characteristic voiceprint features were used to establish training and testing datasets with the ratio of 7:3. The performance of various machine learning algorithms was compared using the training dataset, and the optimal algorithm was used to classify the testing dataset and calculate the sensitivity, specificity, accuracy, and ROC curve.
Results: The three groups showed differences in clustering centers for various voice features such as root mean square energy, power spectral slope, low-frequency percentile energy level, high-frequency spectral slope, spectral harmonic gain, and audio signal energy level. The model of linear SVM showed the best performance in the training dataset, achieving a total accuracy of 95.6% in classifying the three groups in the testing dataset, with sensitivity of 93.3% for MDD, 100% for BD, specificity of 93.3%, AUC of 1 for BD, and AUC of 0.967 for MDD.
Conclusion: By exploring the characteristics of voice features in children and adolescents, machine learning can effectively differentiate between MDD and BD in a population, and voice features hold promise as an objective physiological indicator for the auxiliary diagnosis of mood disorder in clinical practice.
Federated Learning for Collaborative Network Security in Decentralized Environments
Authors: Neyigapula Bheema Shanker
Published: August 2023

In decentralized network environments, collaborative efforts are crucial to bol- stering network security against ever-evolving threats from malicious actors. Federated Learning has emerged as a promising solution, enabling multiple nodes to collectively train machine learning models while preserving data privacy. This research proposes SentinelNet, a novel Federated Learning framework specif- ically designed for collaborative network security. The framework emphasizes secure threat intelligence sharing, privacy-preserving techniques, and adaptive learning mechanisms. Through comprehensive evaluations and real-world case studies, SentinelNet demonstrates its efficacy in enhancing network security while maintaining data confidentiality. The research highlights the significance of col- laborative approaches and advocates the adoption of Federated Learning to fortify decentralized network ecosystems.
Asiaticoside reduces tumor angiogenesis by inhibiting prostate cancer stem cells in an acidic microenvironment
Authors: ZHONG Shaowen , Chen Zhihao , GUO Qijie , HUANG Sheng , XIE Shangyan , HUANG Shuai , WANG Bin
Published: August 2023

Prostate cancer (PCa) is one of the most common malignant tumors in men worldwide. The tumor microenvironment (TME) plays an important role in the development of tumors, and regulation of the acidic TME caused by insufficient tissue perfusion and tumor progression is an important strategy in cancer treatment. However, the effects of an acidic TME on PCa are unknown. In this study, we first examined the effects of an acidic TME on PCa through bioinformatics analysis of the TCGA database, and proposed a prognostic risk assessment model based on the acidic TME that could effectively predict the progression, metastasis and prognosis of PCa. Then, using cell proliferation, matrix adhesion, and Transwell migration and invasion assays, we showed that the acidic TME promoted PCa development and progression. Finally, we demonstrated that Asiaticoside, a traditional Chinese herb, may act as a potential drug to target and inhibit cancer stem cells (CSCs), thereby reducing tumor angiogenesis in PCa. Taken together, this study provided new perspective for the pathogenesis of PCa and highlighted a novel therapeutic strategy that targets PCa CSCs.
Preparation and characterization of poly(lactic acid) (PLA) based poly(ethylene glycol)/eugenia caryophyllus essential oil added smart composite nanofibers for biothermal stabilization
Authors: Ertuğral Tuğba Güngör
Published: August 2023

Poly(ethylene glycol) (PEG) polymer is useful as phase change material (PCM) in thermal energy storage systems. In this study, PEG 1000 and clove essential oil (EO) blended with various mass ratios (100/100/25, 150/100/25, 200/100/25) PEG/PLA/EO nanofibers were produced by electrospinning in a poly(lactic acid) (PLA) polymer matrix. The produced nanofibers were characterized by scanning electronic microscopy (SEM), fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The fibers were about 370°C and prepared nanofibers have a phase enthalpy of 77.48 Jg-1 at 34.74°C. Nanofibers shows promising potential for use in medical, food safety and textile fields.
Surgical Outcomes of Smile Incision and Reverse Shotgun Technique for Distal Interphalangeal Joint Arthrodesis
Authors: Chiang Yi-Sheng , Hsu Cheng-En , Shaw Leo , Chang Chai-Yu , Wang Wei-Chih , Chiu Yung-Cheng
Published: August 2023

Background
Arthrodesis serves as the traditional therapeutic approach for advanced distal interphalangeal joint (DIPJ) arthritis. However, the conventional technique may prove insufficient when the excision of pronounced volar and lateral spurs is required. To address this, we innovated the 'smile incision with reverse shotgun approach'. This method enhances joint exposure and yields superior cosmetic results by extending the transverse skin incision over the DIPJ and cleaving the accessory collateral ligament, thereby improving access to the volar and lateral joint margins. This article meticulously elucidates the surgical procedure and presents preliminary results of its implementation.
Method
The clinical data of 22 consecutive patients (36 fingers) who received DIPJ arthrodesis by this procedure during March 2018 to October 2022, with a mean follow-up period of 9.8 months, were reviewed. Patients’ demographics, union rate, complications, radiographic findings, as well as visual analogue scale (VAS) for pain and satisfaction, were collected and analyzed.
Results
Thirty-five of 36 fingers achieved uneventful bony union (97.2%). The average VAS for pain and satisfaction as well as he coronal plane deviation of DIPJ significantly improved after the operation (all, P < 0.05). No skin necrosis, nail deformity, or infection were observed during the follow-up period.
Conclusion
The smile incision and reverse shotgun approach provided excellent DIPJ exposure, high union rate, and cosmetic appearance for DIPJ arthrodesis surgery. This technique may be a good surgical option for DIPJ arthrodesis when more volar part joint preparation and more volar implant insertion sites are necessary.
Unveiling the emergence of topological quantum phase transitions in the bidirectional transport of carriers through helical edge states in bismuth selenide
Authors: Ukpong Aniekan , Hussien Musa , de Souza Mariano , Tsamparlis Michael
Published: August 2023

Dynamically induced nontrivial band topology in the electronic structure of materials is increasingly being utilized as a primary resource for developing the quantum advantage in emerging technologies. This makes it a fundamental imperative in contemporary condensed matter physics to obtain a deep understanding of the emergence of topological phases during carrier transport in topological matter. In this paper, we have employed a combination of theoretical and computational methods to investigate the emergence of topological quantum transport phases in bismuth selenide and its bias-dependent characteristics by implementing a scalable numerical renormalization group strategy for the carrier transport state. We unravel the emergence of topological quantum phase transitions for carriers hosted on the (001) surface of bismuth selenide because of lattice sublattice asymmetry and spin-orbit coupling and show how the tunnelling transport through the helical surface state is protected against symmetry-breaking perturbations. Our key findings are as follows: (i) charge carriers in bismuth selenide flow bidirectionally through the helical edge states, (ii) the ballistic transport phase undergoes a topological to trivial dynamical phase transition when time reversal symmetry is broken due to an application of a phenomenological field, which may be realized experimentally by impurity doping with ferromagnetic species (iii) quasiparticle interference mediates a transition between different topological quantum phases. These insights are crucial in the rational design of materials for use as interconnects in miniaturized circuits, and manipulation protocols for realizing spontaneous carrier conduction channels using the topological edge states in devices for energy-efficient and lossless transport in microelectronic applications.
Comprehensive analysis of crucial m6A-related differentially expressed genes associated with psoriasis
Authors: Gan Lu , Wu Xuejingzi , Song Jiquan
Published: August 2023

Psoriasis is a chronic inflammatory skin disease that involves genetic and epigenetic factors. N6-methyladenosine methylation (m6A) is the most prevalent RNA modification implicated in various diseases; however, its role in psoriasis remains unclear. Three psoriasis-related datasets were collected from the Gene Expression Omnibus (GEO) database. Differentially m6A methylated genes (DMGs) between psoriasis and controls were identified from the GSE155702 dataset, and corresponding GO and KEGG pathway analyses were performed. Differentially expressed genes (DEGs) and the common DEGs between these two groups were screened from the GSE109248 and GSE142582 datasets. The gene expression of m6A methylation regulators and the interactions among these regulators were also analyzed. 66 significantly upregulated and 381 significantly downregulated m6A peaks were identified corresponding to 414 genes, which were particularly associated with cell and tissue development processes and cell cycle-related items. 271 common DEGs were identified, associating with keratinocyte differentiation, epidermis development, cytokine-cytokine receptor interaction, and fatty acid metabolic processes. 15 crucial m6A-related differentially expressed genes were obtained after the intersection of the DMGs and common DEGs, including NEU2, GALNT6, MTCL1, DOC2B, CAMK2N1, SNTB1, RNF150, CGNL1, CCDC102A, MEOX2, EEF2K, OBSCN, SLC46A2, CCDC85A and DACH1. Our research revealed that psoriasis pathophysiological processes encompass m6A epigenetic alterations, and that m6A alterations may specifically influence cell proliferation and neural regulation and closely associated with osteoarticular involvement and metabolic syndrome in psoriasis.
Identification of NK cell inhibitory ligand CLEC2D-associating immune infiltration landscape and prognostic value in kidney renal clear cell carcinoma
Authors: Chen Cheng , Han Lan , Song Xiujun , Mou Yanhua
Published: August 2023

Background
Kidney renal clear cell carcinoma (KIRC), the most common pathological subtype of RCC, is a characteristics of heterogeneous diseases, and the outcome was dismay with immense diversity. KIRC is obviously resistant to radiotherapy and chemotherapy. In addition, increasing evidence has demonstrated that C-type lectin-like domain family 2 (CLEC2D) expression in cancer contributes to immune escape and thereby emerges as a target for natural killer (NK) cell-mediated immunotherapy. Therefore, we aimed to identify and validate the specific and detailed effect and mechanism of CLEC2D in KIRC.
Methods
CLEC2D expression in KIRC and normal tissues from TCGA were preliminatively screened, and further confirmed in a separate cohort of the GEO. Multivariate analysis and Kaplan-Meier (KM) curves were used to evaluate the relationship between CLEC2D expression and clinicopathologic parameters, and overall survival (OS). Subsequently, noncoding RNAs (ncRNAs) responsible for CLEC2D over-expression were identified by a combination of a series of expression, correlation, and survival analyses. Moreover, we further studied the relationship between CLEC2D expression and immune cell. The compounds were collected from the CellMiner database to examine the significance of CLEC2D in predicting chemotherapy sensitivity.
Results
Contrast to normal tissues, CLEC2D level was significantly higher in KIRC (p < 0.05). Elevated CLEC2D level was tightly correlated with higher grade malignancy and M, N, and tumor stage. Furthermore, univariate and multivariate analyses demonstrated that KIRC cases with high CLEC2D level had shorter OS time than patients with low CLEC2D level (p < 0.05). Subsequently, the LINC00894/-miR-30c-2-3p axis may be considered as the most latent upstream ncRNA-related pathway for CLEC2D in KIRC. Moreover, CLEC2D expression was markedly positively associated with tumor infiltrating immune cells (TIICs), markers of immune cells, and IC. In addition, our research indicated that several drugs IC50s closely related to CLEC2D expression were screened.
Conclusions
In conclusion, abundant expression of CLEC2D was correlated with disease progression and reduced prognosis, and there is sufficient TIICs in KIRC, suggesting that CLEC2D may serve as a promising prognostic biomarker and provide a novel target for tumor immunotherapy of KIRC.
Short-Term Effects of Opioid Use During Therapeutic Hypothermia for Neonatal Encephalopathy
Authors: Jumani Tina , Mishra Priya , Robinson Tonya , Shenberger Jeffrey , Davis Jonathan , Sweigart Benjamin , Turcu Rodica
Published: August 2023

Objective
To examine the effects of opioids during therapeutic hypothermia (TH) on short-term clinical outcomes in neonates with neonatal encephalopathy (NE).
Methods
Multicenter retrospective study of neonates with moderate/severe NE. Opioid exposure was classified as positive (> 0.1 mg/kg) or negative (no exposure or ≤ 0.1 mg/kg) based on cumulative morphine milligram equivalents (MME). Negative binomial regression models were used to evaluate clinical outcomes.
Results
One hundred and twenty neonates were included. Positive opioid exposure was associated with an increase in: 1) length of hospitalization, 2) hypotension/use of vasopressors, and 3) need for and longer duration of mechanical ventilation. Findings were seen with morphine and fentanyl (continuous infusion or intermittent bolus) and were worse with concurrent administration of antiepileptics.
Conclusions
Opioid use during TH was associated with adverse effects on short term clinical outcomes. Caution should be exercised when using opioids during TH until longer-term neurodevelopmental outcome studies can be examined in larger cohorts.
Investigation of Clock Gene Variants in Nannospalax Species in Turkey
Authors: Civelek İlkay , Kankılıç Teoman , Akın Dilara Fatma
Published: August 2023

Blind mole rats (Nannospalax) have become famous for their long lifespans and cancer resistance, but it is still unknown what causes them to be resistant to cancer. These animals may have a different melatonin synthesis mechanism from other creatures due to their lifestyle, which is estimated to be connected to their cancer resistance. In this study, gene variants in Clock genes Cry1, Cry2, Bmal1, Per1 and Per2, which are involved in the synthesis of melatonin in Nannospalax species living in Turkey were investigated and compared with the other organisms including Nannospalax galili, Mus musculus, Heterocephalus glaber, Rattus norvegicus, and Homo sapiens. In addition, it was investigated whether the detected variations have a pathogenic effect in humans. For this purpose, in-vitro methods and some bioinformatics tools were employed in this DNA-based research. 29 variants in total; 11 in Per1, 7 in Per2, 2 in Cry1 and 9 in Cry2 gene, were identified, no variation was found in Bmal1 gene. Some of these variations have been found in regions where DNA repair processes for light-induced UV damage occurred. Other variations were detected in PAS domain and 5'-UTR regions, and the remaining variations were in nondomain regions of proteins. Since Clock gene variations has not been investigated in Nannospalax species, these in-vitro and in-silico methods may give suggestions to choose and focus on detected variations to be used for further studies.
Strengthening of existing pre-engineered building for additional solar panel load using galvanized iron plates
Authors: Kamble Chetana. R. , Patil Rahul. D. , Kumbhar Popat. D. , Desai Piyush. P.
Published: August 2023

In this paper, analysis, and design of an existing PEB installed with solar panels on its roof is performed using STAAD Pro for determining bending stresses to check the structural safety under the action of combined loads viz. dead load, live load, and wind load with additional solar panel load (SL). Failed members due to combined loads with additional SL are identified after the analysis and design by software. To strengthen failed members, analysis and design of PEB were again performed considering galvanized iron plates (GIP) provisions at specified locations. Three different GIP patterns (arrangements) for columns viz. one GIP on one side flange (PC1), one GIP on each flange (PC2), and two GIP at each meeting place of flange and web (PC3) are considered. Similarly, two different GIP patterns for rafters viz. one GIP on bottom flange (PR1) and two GIP at each meeting place of flange and web (PR2) are considered. Results indicate that the bending stresses in the members of PEB with GIP patterns significantly reduced by 56.74% when compared with bending stresses in the members of PEB without GIP patterns. Further, the bending stresses in the columns and rafters of PEB with PC1 and PR1 are found to be lesser by 6.36% and 3.98% respectively when compared with the average bending stresses in the columns and rafters provided with other GIP patterns. The study concludes that the PEB subjected to combined loads with additional SL performs better when its members are strengthened by GIP patterns.
ATP1A1 is a promising new target for melanoma treatment and can be inhibited by its physiological ligand bufalin to restore targeted therapy efficacy
Authors: Soumoy Laura , Genbauffe Aline , Mouchart Lena , Sperone Alexandra , Trelcat Anne , Mukeba-Harchies Léa , Wells Mathilde , Blankert Bertrand , Najem Ahmad , Ghanem Ghanem , Saussez Sven , Journe Fabrice
Published: August 2023

Despite advancements in treating metastatic melanoma, many patients exhibit resistance to targeted therapies. Our study focuses on ATP1A1, a sodium pump subunit associated with cancer development. We aimed to assess ATP1A1 prognostic value in melanoma patients and examine the impact of its ligand, bufalin, on melanoma cell lines in vitro and in vivo. High ATP1A1 expression (IHC) correlated with reduced overall survival in melanoma patients. Resistance to BRAF inhibitor was linked to elevated ATP1A1 levels in patient biopsies (IHC, qPCR) and cell lines (Western blot, qPCR). Additionally, high ATP1A1 mRNA expression positively correlated with differentiation/pigmentation markers based on data from The Cancer Genome Atlas (TCGA) databases and Verfaillie proliferative gene signature analysis. Bufalin specifically targeted ATP1A1 in caveolae (, proximity ligation assay) and influenced Src phosphorylation (Western blot), thereby disrupting multiple signaling pathways (phosphokinase array). In vitro, bufalin induced apoptosis in melanoma cell lines by acting on ATP1A1 (siRNA experiments) and, in vivo, significantly impeded melanoma growth using a nude mouse xenograft model with continuous bufalin delivery via an osmotic pump. In conclusion, our study demonstrates that ATP1A1 could serve as a prognostic marker for patient survival and a predictive marker for response to BRAF inhibitor therapy. By targeting ATP1A1, bufalin inhibited cell proliferation, induced apoptosis in vitro, and effectively suppressed tumor development in mice. Thus, our findings strongly support ATP1A1 as a promising therapeutic target, with bufalin as a potential agent to disrupt its tumor-promoting activity.
Aerobic training with consumption of Nigella sativa extract ameliorate myelin core protein, axonal regeneration, and cognitive function in a mouse model of multiple sclerosis
Authors: lorestani aziz zinvand , Mirnasouri Rahim , rahmati Masoud , darvishi Marzieh
Published: August 2023

Purpose
Multiple sclerosis (MS) is a progressive disease of the central nervous system (CNS) that leads to demyelination of the CNS and memory impairment in animals suffering from this disease. It seems that the release of neurotrophic factors after training is one of the possible mechanisms for increasing axonal protection and brain health of these patients.
methods
In order to induce MS, cuprizone was added to rodent food powder at a weight ratio of 0.2%. The state of balance in the studied groups was checked by repeated measurement statistical test, the level of expression of brain derived neurotrophic factor (BDNF) and myelin basic protein (MBP) markers by immunohistochemistry, the amount of tumor necrosis factor-alpha (TNF-α) by ELISA method and the evaluation of memory and spatial learning by Morris water maze test.
Results
The amount of MBP in the corpus callosum in the cuprizone (CUP) group was significantly decreased compared to the sham group and significantly increased in the groups treated with training and oral Nigella sativa compared to the CUP group. A significant increase in BDNF and a significant decrease in TNF-α were observed in the treated groups compared to the CUP group.
Conclusion
probably due to the reduction of inflammation and the improvement of myelin, the balance and motor activity of the animals have also improved. These structural changes in the brain anatomy of mice, which show histological changes and in line with its behavioral function, can have a new path for faster diagnosis and prevention of debilitating symptoms.
A National Study on the Comparative Burden of Pedestrian Injuries from Falls Relative to Pedestrian Injuries from Motor Vehicle Collisions
Authors: Rundle Andrew G. , Crowe Remle P. , Wang Henry E. , Beard John R. , Lo Alexander X.
Published: August 2023

Pedestrian injuries from falls are an understudied cause of morbidity. Here we compare the burden of pedestrian injuries from falls occurring on streets and sidewalks with that from motor vehicle collisions. Data on injurious falls on streets and sidewalks, and pedestrian-motor vehicle collisions, to which Emergency Medical Services responded, along with pedestrian and incident characteristics, were identified in the 2019 National Emergency Medical Services Information System database. In total, 129,343 injurious falls and 33,910 pedestrians-motor vehicle collisions were identified, with 89% of the incidents occurring in urban areas. Thirty two percent of pedestrians struck by motor vehicles were coded as Emergent or Critical by Emergency Medical Services, while 20% of pedestrians injured by falls were similarly coded. However, the number of pedestrians whose acuity was coded as Emergent or Critical was 2.33 times as high for injurious falls as compared with pedestrians-motor vehicle collisions. This ratio was nearly double at 4.3 for individuals 50 years and older, and almost triple at 6.5 for those 65 years and older. In conclusion, there has been substantial and appropriate policy attention given to preventing pedestrian injuries from motor vehicles, but disproportionately little to pedestrian falls. However, the population burden of injurious pedestrian falls is significantly greater and justifies an increased focus on outdoor falls prevention, in addition to urban design, policy and built environment interventions to reduce injurious falls on streets and sidewalks, than currently exists across the U.S.
Characterization of root hair curling and nodule development in soybean-rhizobia symbioses
Authors: Lu Wei , Wang Xiaochan , Jia Weidong , Ou Mingxiong
Published: August 2023

Aims
Root hair is the initial infection site for symbiotic (rhizobia) process including rhizobia attaching, root hair deforming, and nodule organ forming. Since roots and nodules are blocked by soil and are hard to be perceived, little knowledge is available on the process of soybean root hair deformation and nodule development over time.
Methods
In this study, adaptive microrhizotrons and root hair processing method were used to observe root hairs and to investigate detailed root hair deformation and nodule formation.
Results
It was found that root hairs were not always straight even in uninfected group with relatively small angle (<30°), but root hair curling angle in infected group were large ranging from 32° to 80° since S2 to S6. Nodule was an organ developed late than root hair curling. It initiated from root axis and began to swell in S3, with color changing from light to dark brown in S5. In order to eliminate the observing error, diameter over 1 mm was converted to real diameter with relative formulation. And after conversion, diameter of nodule reached 5 mm in S6. Relationship between root hair curling number/angle and nodule number/diameter indicated that curling angle was strongly related to log nodule diameter (R2 0.84), and curling number was strongly linear to nodule number (R2 0.91).
Conclusions
Thus, nodule number could be calculated through the derived formulation and nodule diameter could be observed and converted to real diameter nondestructively.
Streamlining Email Automation: Seamless Integration of Automation 360 with Outlook and Power Automate Cloud Flows for Continuous Email Monitoring and Unattended Bot Deployment
Authors: Potturu Sai Madhur
Published: August 2023

This research paper explores the integration of Automation 360 platform with Outlook and Power Automate cloud flows to enable continuous monitoring of shared mailbox folders and deploy unattended bots in response to incoming emails. This integration is achieved by leveraging the A360 bot deploy API, allowing different systems to trigger bots. The paper outlines two monitoring and integration methods. The first approach involves setting up VBA code in Outlook to monitor shared mailbox folders and trigger the A360 bot deploy API. This ensures the deployment of unattended bots when emails arrive in designated folders. The second approach utilizes Power Automate cloud flows to monitor shared mailbox folders and deploy unattended bots using the A360 Bot deploy API. The integration of Outlook, Power Automate, and A360 provides several advantages, including timely responses to customer inquiries, improved automation communication, and increased efficiency in email-based processes. Moreover, it optimizes the usage of unattended bot runners, enabling the deployment of scalable and high ROI automation solutions on these runners. By following the outlined steps and leveraging the capabilities of these tools, organizations can streamline their email-based processes, enhance productivity, and achieve greater automation efficiency. The integration of A360 with Outlook and Power Automate provides a comprehensive solution for continuous email monitoring and efficient utilization of automation resources.
Kimura Disease: A Rare Case in Vietnamese Woman
Authors: Le Linh Nguyet , Tran Linh Ngoc Tuong , Pham Duy Le
Published: August 2023

Background: Kimura disease (KD) is a rare benign chronic inflammatory condition that predominantly affects Asian males. It is characterized by subcutaneous tissue masses in the head and neck region, enlarged lymph nodes, increased blood eosinophilia, and elevated serum total IgE levels. In this report, we describe a rare case of KD in a young Vietnamese female.
Case summary: A 31-year-old Vietnamese woman presented to the hospital with 2 masses in the bilateral cheeks and 1 mass behind the left ear that persisted for 15 years, recurrent skin itching, elevated serum total IgE levels and increased blood eosinophilia. No medical history of the individual or family was recorded. We performed an excision biopsy of the postauricular mass which revealed follicular hyperplasia with small vessel hyperplasia, diffuse infiltration of eosinophils in lymphoid follicles, and several eosinophilic microabscesses. After a comprehensive review, the final diagnosis for this patient was KD and atopic dermatitis comorbidity.
Conclusion: KD is not limited to males, as this report demonstrated. The histopathological examination plays an important role in the diagnosis of KD. This case illustrated the characteristic description of KD and highlights the need for awareness of this rare disease in Asian women.