bluuebunny/crossref_metadata_2025 huggingface dataset:
MicroRNA 15a and 16-1 Target Identification in Deletion 13 Multiple Myeloma
Authors: Henry Travis J. , Fonseca Rafael
Published: April 2020

Deletion 13 multiple myeloma (MM) is detected in nearly 50 % of patients diagnosed with MM and confers a shorter survival. A region of minimal deletion was identified for chromosome 13 using Agilent 500K aCGH arrays that included microRNAs 15a and 16- 1. MicroRNAs (miRs) are small RNAs that negatively regulate gene expression through degradation of mRNA transcripts or translational inhibition. In order to determine the contribution of deletion of miRs 15a and 16-1 to MM progression, miR precursors were transfected into KMS-11 and JJN3 adherent myeloma cell lines and total RNA hybridized to Affymetrix U133 Plus 2.0 gene expression arrays for the purpose of identification of mRNA target transcripts. Thirty nanomolar miR 15a and 16-1 precursors and a nonsilencing siRNA control were transfected into adherent KMS-11 and JJN3 myeloma cell lines. Cultures were harvested 16 hours after transfection to minimize the downregulation of transcripts that are not direct targets of miRs 15a and 16-1. Total RNA was extracted using the miRNeasy kit to allow retention of the miR fraction for RT-PCR confirmation of miR over-expression following transfection. Following transfection of miR precursors, expression of miRs 15a and 16-1 were increased 64 and 128-fold, respectively, compared to non-silencing control. Total RNA was hybridized to Affymetrix gene expression arrays using protocols supplied by the manufacturer. Transcripts down-regulated following miR transfection were compared to mathematical models for prediction of miR targets. Additionally, the 3′ UTRs of down-regulated transcripts were inspected for complementarity to miR 15a and 16-1 seed sequences. RT-PCR validation of identified targets was performed. Cross reference of down-regulated transcripts with the TargetSCAN and PictarVERT miR prediction algorithms resulted in a list of 9 genes that represented potential miR-15a/16-1 targets in MM. This list included: FGF2, BCL2, CCNE1, V-MYB, WEE1, E2F7, CDK6, CDC25A and CDC27. Following target identification, reporter constructs were used to confirm direct regulation of transcripts by miRs 15a and 16-1. Functional investigation of miR targets was performed using siRNA reduction of identified targets followed by MTT assay and cell cycle analysis.
Increased Signaling through p62 in the Marrow Microenvironment Increases Myeloma Cell Growth and Osteoclast Formation
Authors: Hiruma Yuko , Kurihara Noriyoshi , Jelinek Diane F. , Roodman David
Published: April 2020

Marrow stromal cells in the bone microenvironment of multiple myeloma (MM) patients play a critical role in promoting both tumor growth and bone destruction. Binding of MM cells to marrow stromal cells, through VCAM-1 on stromal cells and α4β1 integrin on MM cells, results in increased production of TNF-α, receptor activator of NF-KB ligand (RANKL) and IL-6 by marrow stromal cells. These factors in turn increase osteoclast (OCL) formation and the growth of MM cells. Adhesive interaction between MM cells and marrow stromal cells also decrease the sensitivity of MM cells to chemotherapeutic agents. Many of the downstream effects of these adhesive interactions are mediated through the NF-KB, p38 MAPK and JNK signaling pathways. The adapter protein sequestosome-1 (p62) sits at the crossroads of these signaling pathways and mediates the effects of cytokines and other factors that activate NF-KB, p38 MAPK and JNK. However, the role that increased signaling through p62 plays in MM tumor growth and bone destruction is unknown. It is our hypothesis that increased signaling through p62 in marrow stromal cells is necessary for their capacity to increase the growth of MM cells and OCL formation in MM, and thus p62 may be an attractive therapeutic target for MM. To test these hypotheses, we established long-term Dexter-type marrow cultures to isolate marrow stromal cells from MM patients and normals and measured signaling through p62 and PKCζ activation in MM marrow. We found significantly elevated levels of phosho-PKCζ, total PKCζ and VCAM-1 in MM stromal cells. The enhanced IL-6 production resulted from increased p38 MAPK activity and the increased VCAM-1 expression was NF-KB dependent. We then examined the effects of blocking p62 activity in primary patients and normal stromal cells with p62 siRNA (10μg). We confirmed that p62 expression was decreased by at least 90% in both these stromal cells by Western blot analysis. Stromal cells expressing p62siRNA or control siRNA were cultured with or without MM1.S cells for 3 days in separate experiments. Knocking-down p62 in MM derived marrow stromal cells significantly decreased the levels of PKCζ, VCAM-1 and IL-6 in marrow stromal cells and markedly decreased stromal cell support of MM cell growth and OCL formation. Similarly, marrow stromal cells from p62−/− mice produced much lower levels of IL-6, TNF-α, VCAM-1 and RANKL and minimally supported MM cell growth and OCL formation compared to normal cells. Thus, increased signaling through p62 in marrow stromal cells in patients with MM plays an important role in the increased tumor growth and OCL formation in MM, and support p62 as an attractive therapeutic target for MM.
REFRACTORY MEGALOBLASTIC ANEMIA
Authors: DAVIDSON L. S. P. , LOND
Published: April 2020

1. Fifty-nine cases of megaloblastic anemia refractory partially or completely to potent liver extracts given parenterally have been investigated in Edinburgh during the past six years. Thirty-four of these cases were associated with pregnancy, the puerperium or the sprue syndrome. No explanation of the cause of the megaloblastic anemia was discovered in the remaining 25 cases.

2. The etiology, clinical features and treatment of 25 cases of idiopathic refractory megaloblastic anemia are described. Attention is directed to the excellent therapeutic effects produced by proteolysed liver or folic acid.

3. The mechanisms involved in refractoriness to potent parenteral liver extracts are discussed.

4. In certain cases of refractory megaloblastic anemia it is suggested that an unknown hematinic principle, in addition to the liberating factor in purified parenteral liver extract and folic acid, is required for the complete restoration of normoblastic blood formation.
Targeted remodeling of breast cancer and immune cell homing niches by exosomal integrins
Authors: Myint Phyoe Kyawe , Park Eun Jeong , Gaowa Arong , Kawamoto Eiji , Shimaoka Motomu
Published: April 2020

Exosomes represent an important subset of extracellular vesicles involved in inter-cellular communications in health and diseases. Exosomes secreted from cancer and immune cells travel to the specific tissues containing homing niches. The exosomes reaching the niches dynamically modify the gene expression and molecular architectures of the homing niche micro-environments. Cell adhesion molecule integrins regulate the tissue-specific homing patterns of not only cancer and immune cells, but also of the exosomes secreted from those cells. The exosome-mediated remodeling of the homing niches would affect immune lymphocyte migration and host defense, as well as cancer metastasis, thereby representing a potential therapeutic target.
AcademH, a lineage of Academ DNA transposons encoding helicase found in animals and fungi
Authors: Kojima Kenji K.
Published: April 2020

Background

DNA transposons are ubiquitous components of eukaryotic genomes. Academ superfamily of DNA transposons is one of the least characterized DNA transposon superfamilies in eukaryotes. DNA transposons belonging to the Academ superfamily have been reported from various animals, one red algal species Chondrus crispus, and one fungal species Puccinia graminis. Six Academ families from P. graminis encode a helicase in addition to putative transposase, while some other families encode a single protein which contains a putative transposase and an XPG nuclease.

Results

Systematic searches on Repbase and BLAST searches against publicly available genome sequences revealed that several species of fungi and animals contain multiple Academ transposon families encoding a helicase. These AcademH families generate 9 or 10-bp target site duplications (TSDs) while Academ families lacking helicase generate 3 or 4-bp TSDs. Phylogenetic analysis clearly shows two lineages inside of Academ, designated here as AcademH and AcademX for encoding helicase or XPG nuclease, respectively. One sublineage of AcademH in animals encodes plant homeodomain (PHD) finger in its transposase, and its remnants are found in several fish genomes.

Conclusions

The AcademH lineage of TEs is widely distributed in animals and fungi, and originated early in the evolution of Academ DNA transposons. This analysis highlights the structural diversity in one less studied superfamily of eukaryotic DNA transposons.
Stair-climbing gait for a four-wheeled vehicle
Authors: Nakajima Shuro
Published: April 2020

This paper proposes a stair-climbing gait for a four-wheeled vehicle. The idea and methodology of climbing stairs are described, and the results of experiments are shown. Crawler-type vehicles and vehicles with special, complicated mechanisms are commonly used for climbing continuous steps (stairs). In contrast, in this paper, a four-wheeled vehicle with additional degrees of freedom, whose mechanism is not as complicated, is discussed. A gait algorithm based on a pace gait is proposed, and its performance is evaluated.
Lysine-Specific Demethylase 1 Affects the Progression of Papillary Thyroid Carcinoma via HIF1α and microRNA-146a
Authors: Long Miaoyun , Zhu Yue , Chen Zuhe , Lin Shaojian , Peng Xinzhi , Luo Dingyuan , Li Honghao , Tan Langping
Published: April 2020

Context

Lysine-specific demethylase 1 (LSD1) stabilizes hypoxia-inducible factor 1α (HIF1α) to advance tumor progression, while HIF1α functions as a transcription factor to increase the expression of microRNA-146a (miR-146a).

Objective

We aim to investigate whether LSD1 affects the development of papillary thyroid carcinoma (PTC) via HIF1α and miR-146a.

Design

In vitro assays were performed with Nthy-ori 3-1, BHP5-16, BCPAP, K1, and BHP2-7 cell lines. In vivo assays were conducted with established xenograft tumors in nude mice.

Setting

This study was conducted at our lab.

Patients and Materials

PTC tissues and corresponding adjacent normal tissues were obtained from 45 patients hospitalized in Sun Yat-Sen Memorial Hospital. Assays were conducted using Nthy-ori 3-1, BHP5-16, BCPAP, K1, and BHP2-7 cell lines, as well as 50 male BALB/c nude mice.

Intervention

Cells were transfected with sh-LSD1, sh-GABPA, oe-LSD1, oe-HIF1α, miR-146a mimic, and miR-146a inhibitor. In addition, K1 cells expressing lv-oe-LSD1, lv-miR-146a inhibitor, lv-oe-LSD1 or miR-146a inhibitor were injected into the right side of the mice. LSD1 gene and protein expression patterns were analyzed in 45 clinical PTC tissue samples.

Main Outcome Measure

Expression of LSD1, HIF1α, miR-146a, and GA-binding protein transcription factor alpha (GABPA), as well as their effects on PTC.

Results

LSD1 was highly expressed in clinical PTC tissues. LSD1 stabilized HIF1α and inhibited the degradation of its ubiquitin proteasome. HIF1α was enriched in the promoter region of miR-146a, an upregulated miRNA in PTC. HIF1α increased miR-146a expression to promote PTC progression in vitro, which was achieved by inhibiting GABPA, a target gene of miR-146a. LSD1 upregulated miR-146a to enhance the development and metastasis of PTC in nude mice.

Conclusion

Our results show that LSD1 functions as an oncogene in PTC by upregulating HIF1α and miR-146a, elucidating an understanding of undefined mechanisms associated with tumor progression in PTC.
Estradiol Valerate in COC Has More Favorable Inflammatory Profile Than Synthetic Ethinyl Estradiol: A Randomized Trial
Authors: Kangasniemi Marika H , Haverinen Annina , Luiro Kaisu , Hiltunen J Kalervo , Komsi Elina K , Arffman Riikka K , Heikinheimo Oskari , Tapanainen Juha S , Piltonen Terhi T
Published: April 2020

Context

Combined oral contraceptives (COCs) alter inflammatory status and lipid metabolism. Whether different estrogens have different effects is poorly understood.

Objective

We compared the effects of COCs containing ethinyl estradiol (EE) or estradiol valerate (EV) and dienogest (DNG) with those containing DNG only on inflammation and lipid metabolism.

Design

Randomized, controlled, open-label clinical trial.

Setting

Two-center study in Helsinki and Oulu University Hospitals.

Participants

Fifty-nine healthy, young, nonsmoking women with regular menstrual cycles. Age, body mass index, and waist-to-hip ratio were comparable in all study groups at the beginning. Fifty-six women completed the study (EV + DNG, n = 20; EE + DNG, n = 19; DNG only, n = 17).

Interventions

Nine-week continuous use of COCs containing either EV + DNG or EE + DNG, or DNG only as control.

Main Outcome Measures

Parameters of chronic inflammation (high-sensitivity C-reactive protein [hs-CRP], and pentraxin 3 [PTX-3]) and lipid profile (high-density lipoprotein [HDL], low-density lipoprotein [LDL], triglycerides, and total cholesterol).

Results

Serum hs-CRP increased after 9-week use of EE + DNG (mean change ± standard deviation 1.10 ± 2.11 mg/L) compared with EV + DNG (−0.06 ± 0.97 mg/L, P = 0.001) or DNG only (0.13 ± 0.68 mg/L, P = 0.021). Also, PTX-3 increased in the EE + DNG group compared with EV + DNG and DNG-only groups (P = 0.017 and P = 0.003, respectively). In the EE + DNG group, HDL and triglycerides increased compared with other groups (HDL: EE + DNG 0.20 ± 0.24 mmol/L vs EV + DNG 0.02 ± 0.20 mmol/L [P = 0.002] vs DNG 0.02 ± 0.18 mmol/L [P = 0.002]; triglycerides: EE + DNG 0.45 ± 0.21 mmol/L vs EV + DNG 0.18 ± 0.36 mmol/L [P = 0.003] vs DNG 0.06 ± 0.18 mmol/L [P < 0.001]).

Conclusions

EV + DNG and DNG only had a neutral effect on inflammation and lipids, while EE + DNG increased both hs-CRP and PTX-3 levels as well as triglycerides and HDL.

Trial Registration

ClinicalTrials.gov NCT02352090
Familial Longevity is Associated with an Attenuated Thyroidal Response to Recombinant Human Thyroid Stimulating Hormone
Authors: Zutinic Ana , Pijl Hanno , Ballieux Bart E , Roelfsema Ferdinand , Westendorp Rudi G J , Blauw Gerard J , van Heemst Diana
Published: April 2020

Context

Longevity is associated with higher circulating levels of TSH in the absence of differences in circulating thyroid hormones (TH), as previously observed in F2 members of long-lived families (F2-LLS) and their partners (F2-Con). The mechanism underlying this observed difference remains unknown.

Objective

We hypothesized that the thyroid gland of members from long-lived families are less responsive to TSH stimulation, thereby requiring higher circulating TSH levels to maintain adequate TH levels.

Methods

We performed a case-control intervention study with a single intramuscular (gluteal) injection with 0.1 mg recombinant human TSH in a subgroup of 14 F2-LLS and 15 similarly aged F2-Con. They were followed for 4 days. No serious adverse events were reported. For analyses, we compared time trajectories of TSH and TH, and the ratio of TH to TSH using area under the curve (AUC) calculations.

Results

The AUC free T4/AUC TSH ratio was significantly lower in F2-LLS than in F2-Con (estimated mean [95% confidence interval] 1.6 [1.2-1.9] and 2.2 [1.9-2.6], respectively, P = 0.01). The AUC thyroglobulin/AUC TSH ratio was also lower in F2-LLS than in F2-Con (median [interquartile range] 2.1 [1.4-3.6] and 3.2 [2.7-7.4], respectively, P = 0.04). We observed the same trend with the AUC free T3/AUC TSH ratio, although the difference was not statistically significant (estimated mean [95% confidence interval] 0.6 [0.4-0.7] and 0.7 [0.6-0.8], respectively, P = 0.07).

Conclusions

The present findings show that members of long-living families have a lower thyroid responsivity to TSH compared with their partners.
Primary Aldosteronism in the Elderly
Authors: Mulatero Paolo , Burrello Jacopo , Williams Tracy Ann , Monticone Silvia
Published: April 2020

Context

The clinical spectrum and knowledge of the molecular mechanisms underlying primary aldosteronism (PA), the most frequent form of endocrine hypertension, has evolved over recent years. In accordance with the Endocrine Society guidelines and in light of the growing evidence showing adverse cardiovascular outcomes, it is expected that a progressively wider population of patients affected by hypertension will be screened for PA, including the elderly.

Evidence Acquisition

A systematic search of PubMed was undertaken for studies related to the renin-angiotensin-aldosterone system (RAAS), PA, and adrenal histopathology in the elderly population.

Evidence Synthesis

Several studies showed an age-dependent decrease in the activity of RAAS, together with a progressive decrease of the aldosterone response to sodium intake, particularly after the sixth decade of life. The positive correlation between age and serum aldosterone during liberal sodium intake over serum aldosterone during sodium restriction is paralleled by histological changes in adrenal aldosterone synthase (CYP11B2) expression patterns. Immunohistochemical studies showed a progressive loss of the continuous expression of CYP11B2 in the adrenal zona glomerulosa with aging and a concomitant increase of aldosterone-producing cell clusters, which might be responsible for relatively autonomous aldosterone production. Additionally, following PA confirmation and subtype diagnosis, older age is correlated with a lower benefit after adrenalectomy for unilateral PA.

Conclusions

Accumulating evidence suggests that RAAS physiology and regulation show age-related changes. Further studies may investigate to what extent these variations might affect the diagnostic workup of patients affected by PA.
Actions of Peripubertal Gonadal Steroids in the Formation of Sexually Dimorphic Brain Regions in Mice
Authors: Morishita Masahiro , Koiso Ryoma , Tsukahara Shinji
Published: April 2020

The calbindin-sexually dimorphic nucleus (CALB-SDN) and calbindin-principal nucleus of the bed nucleus of the stria terminalis (CALB-BNSTp) show male-biased sex differences in calbindin neuron number. The ventral part of the BNSTp (BNSTpv) exhibits female-biased sex differences in noncalbindin neuron number. We previously reported that prepubertal gonadectomy disrupts the masculinization of the CALB-SDN and CALB-BNSTp and the feminization of the BNSTpv. This study aimed to determine the action mechanisms of testicular androgens on the masculinization of the CALB-SDN and CALB-BNSTp and whether ovarian estrogens are the hormones that have significant actions in the feminization of the BNSTpv. We performed immunohistochemical analyses of calbindin and NeuN, a neuron marker, in male mice orchidectomized on postnatal day 20 (PD20) and treated with cholesterol, testosterone, estradiol, or dihydrotestosterone during PD20-70, female mice ovariectomized on PD20 and treated with cholesterol or estradiol during PD20-70, and PD70 mice gonadectomized on PD56. Calbindin neurons number in the CALB-SDN and CALB-BNSTp in males treated with testosterone or dihydrotestosterone, but not estradiol, was significantly larger than that in cholesterol-treated males. Noncalbindin neuron number in the BNSTpv in estradiol-treated females was significantly larger than that in cholesterol-treated females. Gonadectomy on PD56 had no significant effect on neuron numbers. Additionally, an immunohistochemical analysis revealed the expression of androgen receptors in the CALB-SDN and CALB-BNSTp of PD30 males and estrogen receptors-α in the BNSTpv of PD30 females. These results suggest that peripubertal testicular androgens act to masculinize the CALB-SDN and CALB-BNSTp without aromatization, and peripubertal ovarian estrogens act to feminize the BNSTpv.
Frequency of wrist pain and its associated risk factors in students using mobile phones
Authors: Amjad Fatima , Farooq Muhammad Nazim , Batool Rubia , Irshad Anam
Published: April 2020

Objective: To assess the frequency of wrist pain in students due to mobile phone usage, and impact of usage hours and screen size of mobile phones on pain and disability at wrist joint.

Methods: A cross-sectional survey was conducted among students studying in different universities of Islamabad and Rawalpindi belonging to both public and private sectors. The study was conducted between May 2018 and March 2019. Sample size was 360 students which were selected through convenience sampling. Data was collected through self-formulated closed ended questionnaire. Patient Rated Wrist Evaluation questionnaire was used to assess pain and disability at wrist joint. Data entry and analysis were done using SPSS 21. Results were analyzed using descriptive statistics. Spearman’s and point-biserial correlation coefficients were applied to determine association between different variables.

Results: Point, last month, last 3 months, last 6 months, last year and lifetime frequency were found to be 9%, 18.6%, 29%, 33.3%, 42% and 45.3% respectively. Duration of mobile phone usage was found to be of significant association factor that could lead to wrist pain and disability (p=0.004). Wrist pain was not significantly related to mobile phone screen size (p = 0.488).

Conclusion: It appears that wrist pain is common among mobile phone users and an increase in use of mobile phones increased pain and disability of wrist joint. In addition, it seems that screen size of mobile phone has no significant effect on pain and disability of wrist joint.

doi: https://doi.org/10.12669/pjms.36.4.1797

How to cite this:Amjad F, Farooq MN, Batool R, Irshad A. Frequency of wrist pain and its associated risk factors in students using mobile phones. Pak J Med Sci. 2020;36(4):746-749. doi: https://doi.org/10.12669/pjms.36.4.1797

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Efficient representation of freeform gradient-index profiles for non-rotationally symmetric optical design
Authors: Yang Tianyi , Takaki Nick , Bentley Julie , Schmidt Greg , Moore Duncan T.
Published: April 2020

Conventional optical designs with gradient index (GRIN) use rotationally-invariant GRIN profiles described by polynomials with no orthogonality. These GRIN profiles have limited effectiveness at correcting aberrations from tilted/decentered or freeform systems. In this paper, a three-dimensional orthogonal polynomial basis set (the FGRIN basis) is proposed, which enables the design of GRIN profiles with both rotational and axial variations. The FGRIN basis is then demonstrated via the design of a 3D GRIN corrector plate targeted to correct the rotationally-variant aberrations induced from a tilted spherical mirror. A sample corrector is manufactured and tested, showing significant correction of astigmatism. The FGRIN basis opens a new design space of 3D rotational variant GRIN profiles, which has the potential of replacing multiple freeform surfaces and simplifying complex systems.
Characteristics of Von Bertalanfy Growth, Allometric, Condition Index And Mortality of Periophthalmus barbarus in Mangrove and Probiotics Conservation Area (KKMB), Tarakan, North Kalimantan
Authors: Indarjo Agus , Salim Gazali , Zein Mufrida , Susiyanti Susiyanti , Soejarwo Permana Ari , Nugraeni Christine Dyta , Bija Stephanie , Pham Yen Thi Hong
Published: April 2020

The Mudskipper (P. barbarus) lives in intertidal mud flats and it becomes an indicator of water quality. The transformation of construction and water pollution in this area is important to investigate due to interaction of fishery industry, home residence, and market area surrounding areas. The aim of this research was to analyze the characteristic of Von Bertalanffy growth, allometric, condition index, and mortality of the P. barbarus in KKMB, Tarakan city. The research was designed by using descriptive quantitative method. The sampling process used purposive sampling. The sampling was conducted for 12 times plot.transect-1 in the extension area of KKMB, Tarakan city with a total area is 12 Ha, plot.transect-1area is 10x10 m2, and distance between each transect is 10 m2.  Sampling was carried out in survey area and laboratory to identify the gender and calculate total length and weight. The result showed the growth of male mudskipper (L∞ = 26.545 cm) and female (L∞ = 17.594 cm). Their size and the total population was decreased. The characteristic of male mudskipper growth was positive allometric, then female was negative allometric. The natural mortality and the catch of male mudskipper were higher than female.
Kinetics of Enzymatic Hydrolysis of Passion Fruit Peel using Cellulase in Bio-ethanol Production
Authors: Megawati Megawati , Damayanti Astrilia , Putri Radenrara , Pratama Angga , Muftidar Tsani
Published: April 2020

This research aims to study the hydrolysis of passion fruit peel using cellulase and its evaluation for ethanol production. Passion fruit peel is a fruit processing waste that has not been utilized properly. Passion fruit peel contains holo-cellulose (64% w/w), which can be converted into ethanol through hydrolysis followed by fermentation. Hydrolysis using cellulase is more efficient and its fermentation using yeast to produce ethanol is common. The hydrolysis is carried out at various enzyme ratios (3, 5, 7, and 9% v/v) and at temperature 30 oC, material concentration 5 g/100 mL, pH 4-5, and shaking speed 160 rpm. The kinetics chosen were heterogeneous models; they were the fractal model by Valjamae and Kopelman. Before being hydrolyzed, the essential oil and pectin in passion fruit peel were extracted, because the compositions were quite high; the results were around 16.23 and 11.36% w/w, respectively. The effect of the enzyme ratio to the sugar concentration by hydrolysis is very significant. At 9 h, the glucose concentration reached 45.38, 51.86, 60.50, 66.00 g/L at various enzyme ratios of 3, 5, 7, 9% v/v. During the hydrolysis, the glucose concentration continues to increase and starts to decrease after 9 h. Hydrolyzate solution fermentation obtained from hydrolysis in various enzyme ratios showed consistent results; the higher the enzyme ratio and glucose, and the higher the ethanol will be (5.6, 6.8, 7.6, and 8.9% v/v). The kinetics model by Valjamae is more appropriate to describe the enzymatic hydrolysis mechanism of passion fruit peel than Kopelman. The fractal exponent values obtained from Valjamae and Kopelman models were 0.28 and 0.27. In Valjamae model, the enzyme ratio rises, from 3 to 9% v/v, the rate constant rises from 0.22 to 0.53 1/h. In Kopelman model, the rate constant rises too, from 0.21 to 0.51 1/h.Keywords: bio-ethanol; cellulase; enzymatic hydrolysis; fractal kinetic; passion fruit peel
The Characteristics of Mosque Architecture Based on Public Preferences in Malang City
Authors: Astrini Wulan , Kurniawan E B , Abdillah M
Published: April 2020

Mosque is an architectural work and public facility that serves as a worship place for Muslims. Mosque architectural elements include domes (roofs), mihrab, minaret, arch, and ornaments. Mosque architecture comprises of various elements, which adapt to the local culture where the mosque is located. As such, its presence also bears some impacts on the appearance of urban architecture. Malang city as one of the major cities in East Java has numerous rapidly developing mosque architectures, including Jami’ Great Mosque of Malang, Sabilillah Mosque, Nurul Muttaqin Mosque, Ahmad Yani Mosque, and Manarul Huda Mosque. The meaning of architectural work is also closely related to the perception of humans who observe it. The public preference for the architectural characteristics of mosques in Malang city is a manifestation of architectural work. Conjoined analysis is employed as method operative to analyze public preference. This study aims to find out the architectural characteristics of mosques in Malang city according to public preferences. The findings can serve as a recommendation for the development of mosque architectural designs in Malang city, especially those possessing locality values. The architectural characteristics of mosques in Malang city which are driven by the public preferences ranging from the highest to the lowest, which is why mosques generally have calligraphy ornaments, circular arch, pentagon-shaped minaret, Indo-Persian dome, and two-minaret design.
Seasonal Training Load Quantification and Comparison in College Male Soccer Players
Authors: Ryan Greg A. , Snarr Ronald L. , Eisenman Michelle L. , Rossi Stephen J.
Published: April 2020

Ryan, GA, Snarr, RL, Eisenman, ML, and Rossi, SJ. Seasonal training load quantification and comparison in college male soccer players. J Strength Cond Res 36(4): 1038–1045, 2022—Monitoring and quantification of training load (TL) throughout a competitive soccer season is important to ensure players are able to perform throughout the season. The intent of this study was to examine the positional demands and patterns of select measures of TL during a 14-week season in collegiate male soccer players. Heart rate (HR), running performance (SZ), and perceived recovery data were collected daily using a bioharness for each subject (n = 21). Data were grouped into 2- to 3-week training blocks (Pre1, Pre2, In1, In2, In3, and In4). Continuous variables were analyzed using a multivariate analysis of variance, with post hoc Least Squared Difference pairwise comparisons. Significant positional differences were observed across the season. During Pre1, center midfielders (CM) spent more time in %HRlow compared to center backs (CB) (p < 0.01), wide midfielders (p < 0.01), and center forwards (p = 0.04). Center midfielders spent greater time in SZlower than CB (p < 0.01) and wide backs (WB) (p = 0.01). Wide backs spent greater time in SZupper compared to other positions (all p < 0.01). During Pre2, WB spent more time in %HRhigh and SZupper compared to other positions (all p < 0.01). Positional differences were more varied throughout in-season comparisons, but generally, WB and CB demonstrated higher intensities in variables compared to other positions. Tracking variations in positional TLs across the season is important for coaching and training staffs to determine player readiness and plan future training sessions, while helping to mitigate overuse injuries during a long competitive season.
Stillborn Plots
Authors: Tarr Clayton Carlyle
Published: April 2020

Clayton Carlyle Tarr, “Stillborn Plots: Revolution, Imagination, and the Failure of Romanticism” (pp. 415–447)

The metaphor “fell stillborn from the press” was used frequently by Romantic-era critics to disparage unsuccessful literary productions. Stillbirth statistics began to be collected in earnest in the late eighteenth century alongside an explosion of midwifery texts and obstetric advances. Since Romantic authors often referred to their texts as offspring, it is useful to understand stillbirth plots in these texts as evidence of the authors’ anxiety over imaginative or revolutionary failure. Stillbirth plots are a recurrent theme in Romantic literature, appearing in both novels and poetry, and they are tied together by the mental struggle of the creator, either female or male. In Romantic novels, stillbirth plots signal the defeat of radical theories, whereas in poetry they express the failure of the imagination. Ultimately, these metaphors of failed parturition demonstrate Romantic writers’ fascination with contemporary obstetrics, which made extraordinary advances in the late eighteenth century only to remain static throughout the nineteenth century.
Fungal Extracts as Biocontrol of Growth, Biofilm Formation, and Motility of Xanthomonas citri subsp. Citri
Authors: Luján E.E. , Torres-Carro R. , Fogliata G. , Alberto M.R. , Arena M.E.
Published: April 2020

For the present work, Metarhizium rileyi, a common entomopathogenic fungus, was grown in batch conditions in the absence and presence of its host, Spodoptera frugiperda, to obtain secondary metabolites with potential antimicrobial effect. The extraction of secondary metabolites was carried out by using two solvent systems for the biomass (ethyl acetate and methanol), while secondary metabolites present in the supernatant were extracted by using ethyl acetate as extraction solvent. These extracts were evaluated for their inhibitory effect on the growth, biofilm formation, and motility of three Xanthomonas citri subsp. citri strains (Xcc20, Xcc29, and Xcc42). The in vivo effect of these extracts to prevent the development of cankers on grapefruit leaves was also evaluated. M. rileyi biomass ethyl acetate extracts in the presence and absence of its host had a strong inhibitory effect on Xcc strains growth. On the other hand, the presence of S. frugiperda as an elicitor significantly increased M. rileyi’s inhibitory capacity to form a biofilm. A different behavior was observed for the copper resistant strain, Xcc42, for its biofilm formation and swimming capacity since the most active samples were the supernatant extracts in the presence and absence of S. frugiperda. Our results suggest that the most important factor for in vivo canker development is the inhibition of Xcc’s motility since all the extracts that inhibited canker development in vivo were also able to inhibit Xcc20’s motility in vitro. The fact that the extracts can prevent cankers development in vivo indicates they are promising sources of metabolites to prevent the development of this citrus disease.
A plastic approach to musical meaning: An analysis of The Barbarian by Emerson, Lake & Palmer
Authors: Gecevičiūtė Agnė
Published: April 2020

The aim of the present analysis is discussing a particular piece trying to answer rather impenetrable question of musical meaning. It is assented that in various contexts the same sound can create different meanings and that music can produce different meanings to separate people at different times. Thus music is considered as being more similar to plastic dimension of visual arts than submitting to a figurative reading. Therefore an aesthesic approach – the adaptation of plastic semiotics, which suggests reading the text in a more abstract way – might be also effective to music analysis. This is demonstrated in present analysis where trying to explore musical meaning in a particular piece, development of dynamics, rhythm, tempo, height and duration of sounds, timbres, accents, meter, (in)completeness, pulsation, curves of tension, etc. is discussed.The first piece of the debut album by one of progressive rock founding bands is analysed. It appears that in this object the main ideas of the band and of all progressive rock genre are expressed as well as roles of “high” and “mass” cultures are established.
Two Maps of Rainfall. Semiotic Analysis of Data Visualisation
Authors: Gužauskas Sigitas
Published: April 2020

The article offers a comparison of two visual texts, maps which use different means of expression to visualize a single phenomenon, namely the projected change in rainfall in Europe in the period from 2071 to 2100. These are projections of qualitative data that reflect the relation between a natural phenomenon and a particular geographic location. Rainfall described in figures is presented to the viewer via graphic, verbal, and even material means of expres-sion that help create the impression of the phenomenon’s exist-ence. Map 1 (fig. 1) is a visualisation of rainfall on a completely flat surface created mostly through abstract graphic means of expression. Map 2 (fig. 2, 3, 3a) is a three-dimensional installation consisting of a horizontal map, made of moss and their life-support infrastructure. The latter ensures proper dosage of water and light, thus creating a local ecosystem to control the growth of the moss.The semiotic method of analysis is used to disclose and compare the structure of maps of different expression, their plastic dimension as well as elements of the figurative level. Visual semiotic analysis has been employed here to detect the meaning effects created by different means of expression and identify their differences in the signifying process.The analysis opens up with an overview of the plastic dimension which discusses the chromatic, eidetic, topologic, and haptic characteristics of the map surface. Later, the analysis continues with the overview of the figurative level, focusing on the figures and their thematic values.The last stage of the analysis is dedicated to the generalization of the tensions and oppositions of the plastic level and thematic values of the figures as well as consideration and comparison of the peculiarities related to the perception of meaning.The overall analysis leads to the conclusion that in both maps at-tempts are made to represent the data-based natural phenomenon of rain by means of reinstating the rich sensual experience that was abandoned once the phenomenon was reduced to mathematic values. In addition, it is stated in the conclusions that Map 1 is an object where we can observe constructed signifying system, as meanings here are assigned “artificially”, whereas Map 2 consists of figures of the natural world, namely moss, therefore its language is the natural language.
Stray dogs: Interviews with working-class writers, edited by Daniel M. Mendoza
Authors: Lepley John Walter
Published: April 2020

Stray dogs: Interviews with working-class writers edited by Daniel M. Mendoza. (Down & Out Books: Lutz, Fl., 2016), 147 pages. $6.99 (eBook)
Writing and identity
Authors: Gollobin Stephanie
Published: April 2020

Using a multidisciplinary approach to social justice teaching, this article explores the often invisible impact of double consciousness on adult English language learners in the United States and provides examples of classroom practice that invite students to reflect on its effects. The experience of double consciousness is examined as it relates to English language learner identities. A Critical Language Awareness (CLA) framework and identity-conscious teaching practices are explored to encourage student participation and reflection. This approach, demonstrated through examples used in writing classes, encourages the exploration of identity in the face of oppression by interrogating social constructions and fiction and nonfiction stories containing connected themes. Three classroom lessons and consequent writing are analyzed with a critical discourse lens to examine student responses and reflections on language and identity. Student writing demonstrates that encouraging English language classes to interrogate the language of institutionalized inequity and identity formation can illuminate potential influences of double consciousness, which can empower students to think critically about their identities and choose whether to take steps to mediate the ways in which they could be affected by double consciousness.
Schools, sexual violence, and safety:
Authors: Bhattacharya Usree , McGovern Kathleen R.
Published: April 2020

Two and a half million adolescent girls have experienced some form of sexual violence in India; significantly, they make up a quarter of all rape cases, despite being a small percentage of the population (Raj and McDougal, 2014). Parents and girls’ fears about safety contributes to their high dropout rates within Indian education, but thus far there has been little research on this topic. Focusing on underprivileged adolescent girls at an afterschool site in Mumbai, India, this qualitative study investigates how within this landscape of sexual violence, writing serves as a medium to name, resist, and transform it. Specifically, we scrutinize the articulation of resistance which attempts to contest social norms, cultural conventions, and other forms of everyday hegemony. We examine data extracts from essays written by three adolescent girls participating in the afterschool program as part of a pilot study that took place in December 2016. The analysis of these extracts illuminates how the girls, through their writing, articulate their vulnerabilities about their own and others’ personal safety. Furthermore, it reveals how it is connected to their ability to access education. Moreover, it highlights the ways in which the girls resist parental and other socio-cultural pressures. Finally, the analysis sheds light on the complex and powerful ways in which the girls assert their independence, demand autonomy over their lives, and exercise agency. Ultimately, this investigation offers a path forward for Indian educators to reimagine girls’ education in light of girls’ safety issues, using writing as a space to articulate a literacy of resistance and hope.
Diet of  Odorrana chapaensis  (Bourret, 1937) from Son La Province, Vietnam
Authors: Anh Pham Van , Truong Nguyen Quang , Hoang Nguyen Van
Published: April 2020

Since there is currently a lack of data on the natural history and feeding ecology of Odorrana chapaensis, which was listed in the IUCN Red List (2019), we herein provided the feeding ecology of this amphibian species is virtually lacking. We herein provide data about the diet of O. chapaensis based on the results of our field work in Ngoc Chien Commune, Muong La District and Xim Vang Commune, Bac Yen District, Son La Province, Vietnam. We used the stomach-flushing method to obtain the stomach contents of 85 individuals at two survey sites. A total of 20 prey categories with 334 items, comprising 299 items of invertebrates and 35 unidentified items, were found in the stomachs of O. chapaensis. The dominant prey items of O. chapaensis were Araneae, Polydesmida, insect larvae, Blattodea, Coleoptera, Dermaptera, Lepidoptera, Hymenoptera, and Orthoptera. The importance index for these categories ranged from 3.5% to 32.5%. Coleoptera was the category with the highest frequency of prey items; its representatives were found in 45 stomaches.
New records of  Hipposideros griffini  from lava caves and the threats to its conservation in Vietnam
Authors: Thong Vu Dinh
Published: April 2020

Lava caves are unique and fragile ecosystems which are suitable for a wide variety of plant and animal species. However, the biodiversity of lava caves is still poorly studied. To increase knowledge of these special ecosystems, several rapid surveys on bats were carried out in Dak Nong Province in sounthern Vietnam between 2014 and 2019. Bats were captured in the field using mist nets and harp traps. Echolocation calls were recorded in different situations using the PCTape system. The results include records of Griffin’s leaf-nosed bat (Hipposideros griffini) which is regarded as a species endemic to Vietnam only. Prior to the present study, it was only recorded from karst and moutain habitats in three different regions of Vietnam: Cat Ba National Park in the northeast, Chu Mom Ray in the central highlands, and Cat Tien National Park in the southeast. Records of Hipposideros griffini from Dak Nong Province extend its distributional range and highlight the importance of lava cave systems for biodiversity research and conservation in Vietnam. Griffin’s leaf-nosed bat and other animal species inhabiting the lava caves have been critically threatened by hunting and other human activities. Morphological measurements  and echolocation calls of Hipposideros griffini together with the threats to its conservation status in Dak Nong Province are given in this paper.
Antimicrobial and antioxidant activity of bacterial endophytes isolated from leaves of the mangrove plant Rhizophora stylosa
Authors: Dat Ton That Huu , Oanh Phung Thi Thuy , Tam Vu Thi Thanh , Anh Hoang Le Tuan
Published: April 2020

Mangroves are the most productive ecosystems and contain highly diverse plants and microbial communities. Mangrove endophytes are proved to be a rich source of bioactive secondary metabolites. The biological molecules produced by endophytes play an important role in protection of mangrove plants against herbivores, insects as well as pathogens. The present study aimed to isolate the endophytic bacteria from the mangrove plant Rhizophora stylosa and screen antimicrobial and antioxidant activity of ethyl acetate extracts from the isolated endophytic bacteria. A total of 64 endophytic bacterial strains from R. stylosa leaves were isolated, of which ethyl acetate extracts of 14 isolated endophytic strains showed antimicrobial activity against at least one of reference microorganisms Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 25923, Bacillus subtilis ATCC 27212, Staphylococcus aureus ATCC 12222 and Candida albicans ATCC 7754 with MIC values from 32 to 512 µg/ml. Among them, four strains showed activity against one reference microorganism, five strains showed activity against two reference microorganisms, four strains showed activity against three reference microorganisms, and one strain showed activity against four reference microorganisms. Additionally, the ethyl acetate extracts of 12 isolated endophytic bacteria showed ATBS and DPPH radical scavenging activity with scavenging values from 36.27 ± 2.6% to 71.46 ± 6.6% and from 26.22 ± 3.3% to 57.38 ± 5.8%, respectively. The identification of the five most active endophytic bacteria by 16S rRNA sequences revealed that the endophytes belonged to four genera, including Bacillus, Streptomyces, Pseudovibrio and Pseudomonas. The obtained results suggest that the endophytic bacteria from mangrove plants are a promising reservoir of antimicrobial and antioxidant agents.
Combate ao Coronavírus: a Janela para Intervenções não Farmacológicas é Estreita
Authors: Vasconcelos Giovani L. , Macêdo Antônio M. S. , Ospina Raydonal , Almeida Francisco A. G. , Duarte-Filho Gerson C. , Souza Inês C. L.
Published: April 2020

Nesta nota técnica apresentamos uma breve discussão dos principais resultados do nosso artigo “Modelling fatality curves of COVID-19 and the effectiveness of intervention strategies”, MedRxiv/2020/051557 (DOI:10.1101/2020.04.02.20051557). Nesse artigo, aplicamos o modelo de crescimento de Richards para descrever as curvas de fatalidade da COVID-19 para países que estavam, até 01/04/2020, ou perto do fim ou na fase intermediária da epidemia, como a China, Itália, Espanha e Irã. Também analisamos dados do Brasil, embora ainda estivesse na fase inicial da epidemia, mas nesse caso usamos um modelo alternativo—o modelo de crescimento generalizado—que é mais apropriado para essa fase. Utilizamos ainda o modelo de Richards para estudar a eficácia de possíveis estratégias de intervenção e, nesse contexto, derivamos uma fórmula analítica para a eficiência das estratégias de intervenção não farmacológicas. Os nossos resultados mostram que existe apenas uma estreita janela, após o início do surto, durante a qual intervenções efetivas não farmacológicas podem ser tomadas para conter a epidemia. Nesta nota, apresentamos ainda alguns resultados originais para as curvas de fatalidade da Itália e do Brasil, atualizados com dados até 08/04/2020, além de uma breve descrição geral do trabalho acima mencionado. Para mais detalhes, remetemos o leitor para o artigo original.
Patent-Based News Shocks
Authors: Cascaldi-Garcia Danilo , Vukotić Marija
Published: April 2020

We exploit firm-level data on patent grants and subsequent reactions of stocks to identify technological news shocks. Changes in stock market valuations due to announcements of individual patent grants represent expected future increases in the technology level, which we refer to as patent-based news shocks. Our patentbased news shocks resemble diffusion news, in that they do not affect total factor productivity in the short run but induce a strong permanent effect after five years. These shocks produce positive comovement between consumption, output, investment, and hours. Unlike the existing empirical evidence, patent-based news shocks generate a positive response in inflation and the federal funds rate, in line with a standard New Keynesian model. Patenting activity in electronic and electrical equipment industries, within the manufacturing sector, and computer programming and data processing services, within the services sector, play crucial roles in driving our results.