bluuebunny/crossref_metadata_2025 huggingface dataset:
A framework for the dissemination of research on depression via social media
Authors: Jackson Nathan , Cockrill Antje , Almoraish Ahmed
Published: November 2024

Background/Aims

Effective dissemination of research findings can help to ensure that information reaches the relevant stakeholders, informing evidence-based practice. However, the dissemination of mental health research via widely used channels, such as social media, can be challenging. This study explored the perspectives of researchers (experts) and research users (stakeholders) on the dissemination of depression-related research via social media, aiming to use these insights to create a framework to guide this process.

Methods

Participants were identified using the lead author's professional network. Semi-structured interviews were conducted with five experts (including researchers and one communications officer) and five stakeholders (including mental health practitioners and a member of the public). Interviews were recorded and transcribed verbatim. Data were analysed using thematic analysis.

Results

Experts and stakeholder groups felt that social media could be a useful tool for disseminating research, although this was not always a priority for experts. Barriers to effective dissemination via social media included gaps in expertise, difficulties measuring impact, resource limitations, concerns about negative feedback and differences in stakeholder preferences. Suggestions to optimise dissemination included engaging stakeholders; building knowledge of stakeholders and working with communications teams. Participants generally felt that there was a need for guidance on these processes. The authors used this feedback to create a framework for the dissemination of depression-related research via social media.

Conclusions

Mental health researchers and stakeholders are aware of the potential benefits of social media for the dissemination of depression-related research, but may require more guidance to overcome barriers to effective use of these platforms. A framework could be a useful way of providing this guidance and ensuring that research findings reach key stakeholders.
Prioritising patient experience in the NHS
Authors: Clough Isobel
Published: November 2024

Reflecting on a new article emphasising the strategic importance of patient experience, the editor looks at why concepts such as user experience and reputation are important to public institutions such as the NHS.
Did you know?
Authors: Garlo Nina
Published: November 2024

Nina Garlo examines the close tie between oral health and athletic performance.
Aiming for the best
Authors: Brown Clementine Rees , Baker Linzy
Published: November 2024

Clementine Rees Brown speaks with dental hygienist and therapist Linzy Baker about her work with the ADI.
Pan-TRK positive uterine sarcoma in immunohistochemistry without neurotrophic tyrosine receptor kinase gene fusions: A case report
Authors: Lee Seungmee , Jeon Yu-Ra , Shin Changmin , Kwon Sun-Young , Shin Sojin
Published: November 2024

BACKGROUND

The classification of uterine sarcomas is based on distinctive morphological and immunophenotypic characteristics, increasingly supported by molecular genetic diagnostics. Data on neurotrophic tyrosine receptor kinase (NTRK ) gene fusion-positive uterine sarcoma, potentially aggressive and morphologically similar to fibrosarcoma, are limited due to its recent recognition. Pan-TRK immunohistochemistry (IHC) analysis serves as an effective screening tool with high sensitivity and specificity for NTRK -fusion malignancies.

CASE SUMMARY

We report a case of a malignant mesenchymal tumor originating from the uterine cervix, which was pan-TRK IHC-positive but lacked NTRK gene fusions, accompanied by a brief literature review. A 55-year-old woman presented to the emergency department with abdominal pain and distension, exhibiting significant ascites and multiple solid pelvic masses. Pelvic examination revealed a tumor encompassing the uterine cervix, extending to the vagina and uterine corpus. A punch biopsy of the cervix indicated NTRK sarcoma with positive immunochemical pan-TRK stain. However, subsequent next generation sequencing revealed no NTRK gene fusion, leading to a diagnosis of poorly differentiated, advanced-stage sarcoma.

CONCLUSION

The clinical significance of NTRK gene fusion lies in potential treatment with TRK inhibitors for positive sarcomas. Identifying such rare tumors is crucial due to the potential applicability of tropomyosin receptor kinase inhibitor treatment.
A good index surgery for congenital pseudarthrosis of the tibia minimizes complications following surgery
Authors: Tan Si H. S. , Pei Yingzhi , Chan Chloe X. , Pang Khang C. , Lim Andrew K. S. , Hui James H. , Ning Bo
Published: November 2024

Aims

Congenital pseudarthrosis of the tibia (CPT) has traditionally been a difficult condition to treat, with high complication rates, including nonunion, refractures, malalignment, and leg length discrepancy. Surgical approaches to treatment of CPT include intramedullary rodding, external fixation, combined intramedullary rodding and external fixation, vascularized fibular graft, and most recently cross-union. The current study aims to compare the outcomes and complication rates of cross-union versus other surgical approaches as an index surgery for the management of CPT. Our hypothesis was that a good index surgery for CPT achieves union and minimizes complications such as refractures and limb length discrepancy.

Methods

A multicentre study was conducted involving two institutions in Singapore and China. All patients with CPT who were surgically managed between January 2009 and December 2021 were included. The patients were divided based on their index surgery. Group 1 included patients who underwent excision of hamartoma, cross-union of the tibia and fibula, autogenic iliac bone grafting, and internal fixation for their index surgery. Group 2 included patients who underwent all other surgical procedures for their index surgery, including excision of hamartoma, intramedullary rodding, and/or external fixation, without cross-union of the tibia and fibula. Comparisons of the rates of union, refracture, limb length discrepancy, reoperations, and other complications were performed between the two groups.

Results

A total of 36 patients were included in the study. Group 1 comprised 13 patients, while Group 2 comprised 23 patients. The mean age at index surgery was four years (1 to 13). The mean duration of follow-up was 4.85 years (1.75 to 14). All patients in Group 1 achieved bony union at a mean of three months (1.5 to 4), but ten of 23 patients in Group 2 had nonunion of the pseudarthrosis (p = 0.006). None of the patients in Group 1 had a refracture, while seven of 13 patients who achieved bony union in Group 2 suffered a refracture (p = 0.005). None of the patients in Group 1 had a limb length discrepancy of more than 2 cm, while ten of 23 patients in Group 2 have a limb length discrepancy of more than 2 cm (p = 0.006). In Group 1, four of 13 patients had a complication, while 16 of 23 patients in Group 2 had a complication (p = 0.004). Excluding removal of implants, four of 13 patients in Group 1 had to undergo additional surgery, while 18 of 23 patients in Group 2 had to undergo additional surgery following the index surgery (p = 0.011).

Conclusion

A good index surgery of excision of hamartoma, cross-union of the tibia and fibula, autogenic iliac bone grafting, and internal fixation for CPT achieves union and minimizes complications such as refractures, limb length discrepancy, and need for additional surgeries.
Development of single and multi-coil MR damper subjected to cyclic loading for structural vibration control
Authors:   Hindustan Institute of Technology and Science, Department of Civil Engineering, 603103, Chennai, Tamil Nadu, India, Cruze Daniel , Krishnaraju Nedunchezhian ,   Hindustan Institute of Technology and Science, Department of Civil Engineering, 603103, Chennai, Tamil Nadu, India, Ramalingam Balaji ,   Hindustan Institute of Technology and Science, Department of Civil Engineering, 603103, Chennai, Tamil Nadu, India
Published: November 2024

The magnetorheological (MR) damper can dissipate energy from a vibrating system with low power consumption, making it one of the most popular semi-active damping devices. This study compares single- and multi-coil dampers subjected to cyclic loading. A magnetic oil-based MR fluid is synthesized with 50% carbonyl iron and 50 % synthetic oil, resulting in maximum damping force and minimum sedimentation. A three-dimensional model of the MR damper is created using AutoCAD and is fabricated. Single- and multi-coil MR dampers with a 3000 N capacity are designed and fabricated. The MR dampers are experimentally tested in a universal testing machine with a 1000 kN capacity for various input currents from 1 to 3 A. As the current increases, the damping force also increases in both the single- and multi-coil MR dampers. The multi-coil MR damper has a maximum damping force of 2628 N at a 15 mm amplitude, and the single-coil MR damper has a maximum damping force of 1868 N. A numerical simulation of the magnetic properties of the single- and multi-coil MR dampers is conducted using the finite-element method magnetics. A spring is placed at the top of the piston pole and bottom of the piston to make the MR damper semi-active. The fabricated MR damper can be used in seismic-resistant structures and as a base isolator to protect the structure from vibrations. In addition to civil engineering, it can be used in the development of adaptive prosthetics and orthotic devices, providing dynamic support.
Optical characterization of a manual tunable lens for eye-care applications
Authors: Salvador-Roger Raquel , Esteve-Taboada José J. , Micó Vicente
Published: November 2024

The popularity of focus tunable lenses has increased in the last decade. In this study we present an experimental optical characterization of a commercially available manually tunable lens to describe its behavior regarding optical aberrations, expressed in terms of Zernike coefficients, under different laboratory conditions. Measurements were performed by using a Shack–Hartmann aberrometer, and four different experiments were carried out in order to assess 1) the lens stability in time for a given temperature, 2) the temporal response of the lens, 3) the behavior of the lens when changing the room temperature, and 4) the possible influence of gravity on the lens performance according to its mounting orientation. The main conclusion we outlined states that the properties of the tunable lens stay steady over time as long as room temperature remains constant, making it a good option for ophthalmologic and optometric eye-care applications.
RBD-EVIO: optimized event-based visual-inertial odometry for a large field of view with a rotated binary DART descriptor
Authors: Zhang Yufan , Wang Ze , Shi Hao , Yi Zhonghua , Huang Haoyu , Ye Yaozu , Wang Kaiwei
Published: November 2024

Event-based cameras offer unique advantages over traditional cameras, such as high dynamic range, absence of motion blur, and microsecond-level latency. This paper introduces an innovative approach to visual odometry, to our knowledge, by integrating the newly proposed Rotated Binary DART (RBD) descriptor within a Visual-Inertial Navigation System (VINS)-based event visual odometry framework. Our method leverages event optical flow and RBD for precise feature selection and matching, ensuring robust performance in dynamic environments. We further validate the effectiveness of RBD in scenarios captured by a large field-of-view (FoV) fisheye event camera under high dynamic range and high-speed rotation conditions. Our results demonstrate significant improvements in tracking accuracy and robustness, setting what we believe to be a new benchmark for event-based visual odometry. This work paves the way for advanced applications in high-speed, high dynamic range, and large FoV visual sensing.
The nature of multi-channel color perception from three photodetector types with wide overlapping spectral sensitivity bands
Authors: Diaconu Vasile , Faubert Jocelyn
Published: November 2024

Human color perception generates typical chromatic sensations from various wavelengths of the visible spectrum by exciting three broadband sensitivity photodetector types. The retinal neural network compares, differentiates, and redirects signals from the three photodetectors to the cortex through several chromatic pathways. The current concept that explains the creation of the different color pathways through the retina's neural network is not compatible with its implementation in a physical instrument to characterize colors in a way similar to the visual system so the mechanism of neural color-coding of the retina is partially known. The present paper presents a physical process of de-multiplexing signals emitted by two or more types of photodetectors with a wide band of overlapping spectral sensitivities to differentiate signals corresponding to the spectral zones that the photodetectors share, and signals corresponding to the distinctive spectral sensitivity zone of each photodetector. The model adopts two fundamental principles from retinal neuron signal processing, such as the contrast of photoreceptor signals, as well as the ON and OFF properties of the retina's neural network to redirect the contrasts of photoreceptor signals to different chromatic channels. The concept of this model represents a good alternative to explain the process of color coding signals from three types of retinal photodetectors to activate the different channels involved in human color perception.
Polarization-based selective photoionization of ⁶⁷Zn
Authors: Steenkamp Christine , Waso Frederick
Published: November 2024

Isotope selective photoionization of ⁶⁷Zn is demonstrated with broadband excitation light (∼ 3.4 GHz), temporally separated laser pulses in the presence of a weak external magnetic field. Starting with natural zinc metal (∼ 4% ⁶⁷Zn) the ion sample was enriched to ∼ 90% ⁶⁷Zn. Efficient suppression of the ionization of the even-mass isotopes was achieved by intermediate state alignment in the excitation scheme 4𝑠2 ¹𝑆₀ → 4𝑠4𝑝 ³𝑃₁ → 4𝑠4𝑑 ³𝐷₁, followed by nonresonant photoionization. The first excitation pulse was linearly polarized parallel to the transverse component of the external magnetic field and the linear polarization of the second excitation pulse was rotated to compensate for Larmor precession of the quantization axis during the temporal delay between the two excitations. This is the first systematic study, in the context of resonance ionization, investigating the independent and continuous rotation of the polarizations of the excitation pulses and demonstrating efficient compensation for the presence of an external magnetic field that has an arbitrary direction. Experimental results are supported by a vector model combined with rate equations.
The strength of interspecies interaction in a microbial community determines its susceptibility to invasion
Authors: Muzafar Suraya , Nair Ramith R. , Andersson Dan I. , Warsi Omar M.
Published: November 2024

Previous work shows that a host’s resident microbial community can provide resistance against an invading pathogen. However, this community is continuously changing over time due to adaptive mutations, and how these changes affect the invasion resistance of these communities remains poorly understood. To address this knowledge gap, we used an experimental evolution approach in synthetic communities of Escherichia coli and Salmonella Typhimurium to investigate how the invasion resistance of this community against a bacterium expressing a virulent phenotype, i.e., colicin secretion, changes over time. We show that evolved communities accumulate mutations in genes involved in carbon metabolism and motility, while simultaneously becoming less resistant to invasion. By investigating two-species competitions and generating a three-species competition model, we show that this outcome is dependent on the strength of interspecies interactions. Our study demonstrates how adaptive changes in microbial communities can make them more prone to the detrimental effects of an invading species.
Combining OpenStreetMap mapping and route optimization algorithms to inform the delivery of community health interventions at the last mile
Authors: Randriamihaja Mauricianot , Ihantamalala Felana Angella , H. Rafenoarimalala Feno , Finnegan Karen E. , Rakotonirina Luc , Razafinjato Benedicte , H. Bonds Matthew , V. Evans Michelle , Garchitorena Andres
Published: November 2024

Community health programs are gaining relevance within national health systems and becoming inherently more complex. To ensure that community health programs lead to equitable geographic access to care, the WHO recommends adapting the target population and workload of community health workers (CHWs) according to the local geographic context and population size of the communities they serve. Geographic optimization could be particularly beneficial for those activities that require CHWs to visit households door-to-door for last mile delivery of care. The goal of this study was to demonstrate how geographic optimization can be applied to inform community health programs in rural areas of the developing world. We developed a decision-making tool based on OpenStreetMap mapping and route optimization algorithms in order to inform the micro-planning and implementation of two kinds of community health interventions requiring door-to-door delivery: mass distribution campaigns and proactive community case management (proCCM) programs. We applied the Vehicle Routing Problem with Time Windows (VRPTW) algorithm to optimize the on-foot routes that CHWs take to visit households in their catchment, using a geographic dataset obtained from mapping on OpenStreetMap comprising over 100,000 buildings and 20,000 km of footpaths in the rural district of Ifanadiana, Madagascar. We found that personnel-day requirements ranged from less than 15 to over 60 per CHW catchment for mass distribution campaigns, and from less than 5 to over 20 for proCCM programs, assuming 1 visit per month. To illustrate how these VRPTW algorithms can be used by operational teams, we developed an "e-health" platform to visualize resource requirements, CHW optimal schedules and itineraries according to customizable intervention designs and hypotheses. Further development and scale-up of these tools could help optimize community health programs and other last mile delivery activities, in line with WHO recommendations, linking a new era of big data analytics with the most basic forms of frontline care in resource poor areas.
Repeat mediated excision of gene drive elements for restoring wild-type populations
Authors: Chennuri Pratima R. , Zapletal Josef , Monfardini Raquel D. , Ndeffo-Mbah Martial Loth , Adelman Zach N. , Myles Kevin M.
Published: November 2024

Here, we demonstrate that single strand annealing (SSA) can be co-opted for the precise autocatalytic excision of a drive element. We have termed this technology Repeat Mediated Excision of a Drive Element (ReMEDE). By engineering direct repeats flanking the drive allele and inducing a double-strand DNA break (DSB) at a second endonuclease target site within the allele, we increased the utilization of SSA repair. ReMEDE was incorporated into the mutagenic chain reaction (MCR) gene drive targeting the yellow gene of Drosophila melanogaster, successfully replacing drive alleles with wild-type alleles. Sequencing across the Cas9 target site confirmed transgene excision by SSA after pair-mated outcrosses with yReMEDE females, revealing ~4% inheritance of an engineered silent TcG marker sequence. However, phenotypically wild-type flies with alleles of indeterminate biogenesis also were observed, retaining the TGG sequence (~16%) or harboring a silent gGG mutation (~0.5%) at the PAM site. Additionally, ~14% of alleles in the F2 flies were intact or uncut paternally inherited alleles, indicating limited maternal deposition of Cas9 RNP. Although ReMEDE requires further research and development, the technology has some promising features as a gene drive mitigation strategy, notably its potential to restore wild-type populations without additional transgenic releases or large-scale environmental modifications.
Perceptions of healthcare finance and system quality among Nigerian healthcare workers
Authors: Josiah Blessing Osagumwendia , Enebeli Emmanuel Chukwunwike , Duncan Brontie Albertha , Anukam Lordsfavour Uzoma , Akingbade Oluwadamilare , Ncube France , Josiah Chinelo Cleopatra , Alimele Eric Kelechi , Otoboyor Ndidi Louis , Josiah Oghosa Gabriel , Mukoro Jemima Ufuoma , Nganwuchu Blessing Chiamaka , Opeyemi Fawole Israel , Olaosebikan Timothy Wale , Kantaris Marios
Published: November 2024

Nigeria’s healthcare system faces significant challenges in financing and quality, impacting the delivery of services to its growing population. This study investigates healthcare workers’ perceptions of these challenges and their implications for healthcare policy and practice. A cross-sectional survey was conducted with 600 healthcare professionals from eight states across Nigeria, representing a variety of healthcare occupations. Participants completed a questionnaire that assessed their perceptions of healthcare financing, quality of care, job satisfaction, and motivation using a 5-point Likert scale, closed- and open-ended questions. Descriptive statistics, Chi-squared test, and regression analysis were used to analyze the data. The findings revealed that healthcare workers were generally not satisfied with the current state of healthcare financing and system quality in Nigeria. Poor funding, inadequate infrastructure, insufficient staffing, and limited access to essential resources were identified as major challenges. These challenges contributed to low job satisfaction, demotivation, and a desire to leave the profession. Socioeconomic factors, location State of practice, professional designation (clinical vs nonclinical), clinical designation (profession), and employment type (full-time vs part-time) were found to influence healthcare workers’ perceptions (p < 0.05). The findings indicated a need to improve healthcare workers’ satisfaction and retention, and quality of care in Nigeria, by increasing healthcare funding, transparent fund management protocols, investing in infrastructure and human resource development, and addressing regional healthcare disparities. By implementing these reforms, Nigeria can enhance the quality and accessibility of healthcare services and improve the health and well-being of its citizens.
First evidence of circulation of multiple arboviruses in Algeria
Authors: Boubidi Saïd C. , Mousson Laurence , Kernif Tahar , Khardine Fayez , Hachid Aïssam , Beck Cécile , Lecollinet Sylvie , Moraes Rayane A. , Moutailler Sara , Dauga Catherine , Failloux Anna Bella
Published: November 2024

Background

Algeria like other North African countries is experiencing recurrent episodes of West Nile Virus (WNV) emergences and new health threats associated with the introduction of Aedes albopictus in 2010 are to be feared. To improve the surveillance of mosquito-borne pathogens, we performed a study using innovative tools based on multiplex molecular methods.

Methods

We combined two approaches: a high-throughput chip based on the BioMark Dynamic array system to detect arboviruses in mosquitoes, and a set of immunologic methods (ELISA, microsphere immunoassays (MIA) and virus microneutralization tests (MNT)) for serological surveys in animal hosts. We investigated two distinct regions: a first zone located in the coastal humid region and a second one in the Saharan desert region.

Principal findings

We collected a total of 1,658 mosquitoes belonging to nine different species and found predominantly Culex pipienss. l. (56.5%) and Cx. perexiguus (27.5%). From 180 pools of 10 mosquitoes, we detected four arboviruses: Banna virus (BAV), chikungunya virus (CHIKV), Sindbis virus (SINV), and Usutu virus (USUV). Moreover, we examined 389 blood samples from equids and poultry and found that 52.4% were positive for flavivirus antibodies in ELISA, while 30.8% were positive for WNV and two chickens and two equids were positive for USUV by MNT and MIA respectively.

Conclusions

To our knowledge, this is the first report of five arboviruses circulating in Algeria, with three reported for the first time (CHIKV, BAV, and USUV). Our study brings evidence that reinforcing surveillance using more discriminant tools may help in anticipating future emergences and propose adapted control measures.
Suitability of anthrax (Bacillus anthracis) in the Black Sea basin through the scope of distribution modelling
Authors: Arede Margarida , Allepuz Alberto , Beltran-Alcrudo Daniel , Casal Jordi , Romero-Alvarez Daniel
Published: November 2024

The Black Sea basin has a strategic geographic location bridging Asia and Europe and depends on traditional livestock practices for their local economies. Anthrax, a zoonotic bacterial disease caused by Bacillus anthracis, poses a significant global threat impacting public health, food security, pastoralist communities, and national economies. The disease is endemic or sporadic in the Black Sea basin, however, the study of its distribution has seldom been addressed, despite its burden and the presence of historical B. anthracis burial sites in the region. The viability of B. anthracis in a particular region is going to be influenced by multiple environmental factors, such as soil composition, climate, vegetation, and host abundance. For characterizing the potential distribution of B. anthracis in the Black Sea basin and assessing the potential for anthrax outbreaks, we applied an ecological niche modelling framework using the Maxent algorithm. This machine-learning algorithm models species distributions based on presence data and background information from a specified calibration region. We analyzed multiple variable combinations and proposed a novel approach for interpreting in-risk anthrax areas. Our findings underscored the importance of host abundance to the anthrax dynamics in the region. We identified anthrax-suitable areas spanning central and eastern Türkiye, Armenia, southern Georgia, southern Russia, Bulgaria, southern and eastern Romania, Hungary, Moldova, and southern Ukraine, which align with findings from previous global and regional studies on the potential suitability of anthrax. The insights gained from our research may help to develop targeted interventions, such as awareness and educational campaigns about anthrax, supervision of anthrax-infected carcasses disposal, and the promotion of livestock vaccination in high-risk areas. Additionally, these results can inform policies to mitigate the spread of anthrax in pastoralist communities in the Black Sea basin and foster collaboration between veterinary and public health entities on anthrax control.
Natural field diagnosis and molecular confirmation of fungal and bacterial watermelon pathogens in Bangladesh: A case study from the Natore and Sylhet districts
Authors: Ferdous Raihan
Published: November 2024

The study investigated watermelon diseases characterized by contrasting climatic conditions in the Sylhet and Natore Districts of Bangladesh. Sylhet experiences lower temperatures and high rainfall, while Natore has higher temperatures and low rainfall. In these survey regions, 40 watermelon fields were selected, and 10 diseases were observed, including 4 fungal, 3 bacterial, 2 water mold, and 1 viral disease. The observed diseases were Anthracnose, Cercospora leaf spot, Fusarium wilt, Gummy stem blight, Downy mildew, Phytophthora fruit rot, Bacterial fruit blotch, Angular leaf spot, Yellow vine, and Watermelon mosaic disease. Molecular analysis was done in the Plant Pathology Lab at Sher-e-Bangla Agricultural University using the specific primers for fungal (ITS1/ITS4) and bacterial (27F/1492R) DNA regions and identified nine pathogen species, excluding the causal organism of the viral disease. The identified pathogens included Colletrotrichum orbiculare, Cercospora citrullina, Fusarium oxysporum, Stagonosporopsis cucurbitacearum, Pseudoperonospora cubensis, Phytophthora capsici, Acidovorax citrulli, Pseudomonas syringae, and Serratia marcescens. The sequencing of the identified pathogens revealed high homology (98.91–99.71%) with known sequences in the GenBank database. Phylogenetic analysis showed six clusters for fungal and water mold pathogen isolates and three for bacterial isolates where the percentages of replicate trees were 100% in all the cases. Among the identified diseases, the highest disease occurrence was caused by Fusarium wilt (47.5%) followed by Gummy stem blight (41.5%) in the Sylhet region and Angular leaf spot (37.5%) followed by Yellow vine (33%) in the Natore area. Fusarium wilt also has a high disease intensity, demonstrating its devastating impact on yield. This study highlights the influence of environmental conditions on disease incidence and underscores the need for tailored management strategies. These findings provide a foundation for developing targeted disease management practices for sustainable watermelon cultivation in Bangladesh.
Understanding the success factors of MOOCs’ retention intention: A Necessary Condition Analysis
Authors: Huacui Zhang , Hossain Md. Nahin , Zhen Kong , Kumar Nilesh
Published: November 2024

Purpose

This paper seeks to explore the influence of success factors, specifically motivation and course quality, on MOOC retention intention. Going beyond a mere examination of these motivational and quality factors, the study investigates students’ motivation, considering needs, interests, course system, content, and service quality. Methodologically, a questionnaire survey was conducted, collecting data from 311 students enrolled in online courses. To ascertain the impact of interest or need-based motivation on students’ retention rates, a Structural Equation Model (SEM) was employed. Subsequently, Necessary Condition Analysis (NCA) was utilized to identify the essential factors and components in each area. SEM results revealed a positive influence of motivational factors and quality issues on students’ behavior. Retention behavior was notably affected by academic and professional needs, along with personal interests. Furthermore, course content and service quality demonstrated a significant effect on students’ perseverance behavior. NCA results identified academic motivation and system quality as having a substantial impact on retention behavior, while personal motivation and technological motivation had a comparatively smaller effect. Practically, the findings suggest that course developers should consider students’ academic and personal requirements when designing online courses. Additionally, providing students with the ability to customize course and system content according to their needs is crucial. Timely problem-solving attitudes from service providers are essential for ensuring student retention.
Treated and untreated cows housed side by side in tie-stalls and their respective risk of harboring E. coli resistant to antimicrobials
Authors: Köchle Belinda , Bernier Gosselin Véronique , Kaspar Heike , Becker Jens
Published: November 2024

Parenteral antimicrobial treatment results in the excretion of antimicrobial-resistant bacteria. Dairy cows are commonly housed side by side in tie-stalls and often receive antimicrobial treatment. However, studies investigating treated cows as source of colonization of neighboring cows with resistant bacteria are scarce. Antimicrobial resistance (AMR) in cows (treated and untreated) in tie-stalls was investigated to assess their respective risks of carrying resistant bacteria. Furthermore, we analyzed associations of farm management with AMR. Case-control study: For isolation of indicator Escherichia (E.) coli, rectal swab samples were taken. Cows were sampled depending on treatment history and proximity to one another (cow A: recently treated parenterally; cow B: untreated, next to cow A; cow C: untreated, at considerable distance from all treated cows). Antimicrobial susceptibility was tested by microdilution. Associations of AMR with exposure to cow A, treatments, and management were analyzed using generalized mixed-effects logistic models. Susceptibility data on 571 isolates from 131 dairy farms were obtained. Almost no difference in proportions of resistant E. coli was observed between cows B and C (B: 53.4%; C: 57.2%; P = 0.52). Untreated cows had lower odds of carrying resistant E. coli than treated cows (B: OR 0.44, P<0.001; C: OR 0.54, P = 0.007). Non-pansusceptibility of isolates was associated with antimicrobial treatment (1 treatment: OR 2.11, P = 0.001; ≥2: OR 1.76, P = 0.043). Using manure on forage crops was associated with higher odds of pansusceptibility (OR 2.01, P = 0.004). For daily practice, with regard to the risk of AMR transmission, results of this study do not provide evidence for the need to separate treated cows from others during treatment in tie-stalls.
Enhancing precision flood mapping: Pahang’s vulnerability unveiled
Authors: Keya Tahmina Afrose , Balakrishnan Siventhiran S. , Solayappan Maheswaran , Dheena Dhayalan Saravana Selvan , Subramaniam Sreeramanan , An Low Jun , Leela Anthony , Fernandez Kevin , Kumar Prahan , Lokeshmaran A. , Boratne Abhijit Vinodrao , Abdullah Mohd Tajuddin
Published: November 2024

Malaysia, particularly Pahang, experiences devastating floods annually, causing significant damage. The objective of the research was to create a flood susceptibility map for the designated area by employing an Ensemble Machine Learning (EML) algorithm based on geographic information system (GIS). By analyzing nine key factors from a geospatial database, flood susceptibility map was created with the ArcGIS software (ESRI ArcGIS Pro v3.0.1 x64). The Random Forest (RF) model was employed in this study to categorize the study area into distinct flood susceptibility classes. The Feature selection (FS) method was used to ranking the flood influencing factors. To validate the flood susceptibility models, standard statistical measures and the Area Under the Curve (AUC) were employed. The FS ranking demonstrated that the primary attributes to flooding in the study region are rainfall and elevation, with slope, geology, curvature, flow accumulation, flow direction, distance from the river, and land use/land cover (LULC) patterns ranking subsequently. The categories of ’very high’ and ’high’ class collectively made up 37.1% and 26.3% of the total area, respectively. The flood vulnerability assessment of Pahang found that the Eastern, Southern, and central regions were at high risk of flooding due to intense precipitation, low-lying topography with steep inclines, proximity to the shoreline and rivers, and abundant flooded vegetation, crops, urban areas, bare ground, and rangeland. Conversely, areas with dense tree canopies or forests were less susceptible to flooding in this research area. The ROC analysis demonstrated strong performance on the validation datasets, with an AUC value of >0.73 and accuracy scores exceeding 0.71. Research on flood susceptibility mapping can enhance risk reduction strategies and improve flood management in vulnerable areas. Technological advancements and expertise provide opportunities for more sophisticated methods, leading to better prepared and resilient communities.
Isoscopoletin inhibits hepatocellular carcinoma cell proliferation via regulating glycolysis-related proteins
Authors: Ruan Chenyao , Wang Chen , Gu Jiawen , Zhu Zhihui
Published: November 2024

Objective

Isoscopoletin is one of the primary metabolites of natural product scoparone, which was reported to against tumor proliferation. The aim of this study was to explore the mechanism of isoscopoletin against hepatocellular carcinoma (HCC).

Methods

Transcriptomics was used to reveal the possible pathways of isoscopoletin against HCC in vitro. The potential targets of isoscopoletin against HCC through affecting glycolysis were analyzed by network pharmacology, then the potential binding abilities of isoscopoletin to glycolysis-related proteins were initially verified by high throughput virtual molecular docking. The affinities of isoscopoletin for glycolysis-related proteins were assayed using microscale thermophoresis (MST), which was reverse-validated by inhibiting the binding ability of isoscopoletin to GPD2. Glucose consumption and lactate production were examined to evaluate the effects of isoscopoletin on intracellular glycolysis, and the regulation of glycolysis-related targets by isoscopoletin was detected using RT-qPCR and ELISA kits.

Results

The results of transcriptomics showed that the differentially expressed genes (DEGs) were mainly enriched in glycolysis and other metabolic-related pathways. Network pharmacology and molecular docking revealed that GPD2, GPI, HSP90AA1 and PGK2 were the core targets in the glycolysis process of isoscopoletin against HCC. MST results showed that there was a strong affinity between isoscopoletin and GPD2, GPI, Hsp90α and PGK2. In vitro results showed that isoscopoletin inhibited glucose consumption and lactate production, while regulating the levels of glycolysis-related proteins.

Conclusion

This study suggests that isoscopoletin may exist an anti-tumor effect by regulating the glycolysis-related proteins GPD2, GPI, Hsp90α and PGK2, inhibiting the glycolysis process in HCC cells, then blocking the energy supply of tumor cells.
Research on virtual reality-based assessment framework and application path in medical education
Authors: Wang Yue , Li Yan , Chen Chen , Zhang Wenli , Wang Yaping , Sha Kun , Wang Shiyong
Published: November 2024

While virtual reality(VR) technology enhances learning, it also places new demands on medical learning evaluation. Verifying the occurrence of learning is a primary issue. To design and implement practical and feasible VR-based learning evaluation based on the immersive learning evaluation framework, the Substitution-Augmentation-Modification-Redefinition (SAMR) model, a VR-based learning evaluation framework, was constructed. This framework included competency, learning objectives, assessment tasks, evaluation data, criteria, and feedback. A comprehensive application pathway was developed, utilizing technological integration frameworks. This pathway includes the selection and implementation processes to offer teachers theoretical direction on evaluating medical learning using VR. Finally, this study performed a learning evaluation utilizing VR. The findings revealed that using VR for evaluation can create a deeply engaging and interactive environment. Participants reported feeling a strong sense of being present in the virtual environment and expressed high acceptance and satisfaction with the VR evaluation process. Furthermore, they believed that VR evaluation offers a comprehensive and practical means of assessing cognitive abilities and receiving feedback. These findings establish that VR evaluation optimise learning assessment and showcase the feasibility of the assessment framework and application path.
n-Butylidenephthalide recovered calcium homeostasis to ameliorate neurodegeneration of motor neurons derived from amyotrophic lateral sclerosis iPSCs
Authors: Deng Yu-Chen , Liu Jen-Wei , Ting Hsiao-Chien , Kuo Tzu-Chen , Chiang Chia-Hung , Lin En-Yi , Harn Horng-Jyh , Lin Shinn-Zong , Chang Chia-Yu , Chiou Tzyy-Wen
Published: November 2024

Amyotrophic lateral sclerosis (ALS) is an incurable neurodegenerative disease that causes muscle atrophy and primarily targets motor neurons (MNs). Approximately 20% of familial ALS cases are caused by gain-of-function mutations in superoxide dismutase 1 (SOD1), leading to MN degeneration and ion channel dysfunction. Previous studies have shown that n-Butylidenephthalide (BP) delays disease progression and prolongs survival in animal models of ALS. However, no studies have been conducted on models from human sources. Herein, we examined the protective efficacy of BP on MNs derived from induced pluripotent stem cells (iPSCs) of an ALS patient harboring the SOD1G85R mutation as well as on those derived from genetically corrected iPSCs (SOD1G85G). Our results demonstrated that the motor neurons differentiated from iPSC with SOD1G85R mutation exhibited characteristics of neuron degeneration (as indicated by the reduction of neurofilament expression) and ion channel dysfunction (in response to potassium chloride (KCl) and L-glutamate stimulation), in contrast to those derived from the gene corrected iPSC (SOD1G85G). Meanwhile, BP treatment effectively restored calcium ion channel function by reducing the expression of glutamate receptors including glutamate ionotropic receptor AMPA type subunit 3 (GluR3) and glutamate ionotropic receptor NMDA type subunit 1 (NMDAR1). Additionally, BP treatment activated autophagic pathway to attenuate neuron degeneration. Overall, this study supports the therapeutic effects of BP on ALS patient-derived neuron cells, and suggests that BP may be a promising candidate for future drug development.
A protocol for modeling the factors influencing the deployment of the COVID-19 vaccine across African countries
Authors: Ezezika Obidimma , Kotsaftis Tiana Stephanie , Amponsah-Dacosta Edina , Demi Suleyman , Omwenga Eric Omori , Mong’are Samuel , Zaranyika Trust , Ariyo Oluwaseun , Ngianga-Bakwin Kandala , Ameyaw Edward Kwabena
Published: November 2024

Evidence fails to capture disparities amongst African countries in terms of the measure of administered COVID-19 vaccine doses per 100 people. Assessment of data on doses secured, administered, and supplied was undertaken to investigate quantitative measures that impacted COVID-19 vaccine deployment, thereby emphasizing distribution and supply indicators. We employ a full linear regression to identify independent variables that have an impact on vaccination rates, including macroeconomic indicators such as World Bank Income Classification, Gross Domestic Product (GDP) per capita and various indices such as Health Access and Quality Index, Human Development Index, Global Peace Index, Education Index, Political Stability Index and Government Effectiveness. This analysis aims to construct a statistical model utilizing regression analysis to identify key drivers of COVID-19 vaccine deployment in Africa and offer insights into vaccination disparities in the continent. Recognizing the global importance of achieving high vaccination rates, the study sheds light on specific challenges faced by individual countries within Africa, thereby emphasizing the need for tailored efforts. Beyond COVID-19, the research contributes to understanding the relationship between vaccination rates and social indicators that, potentially impact broader public health concerns and global vaccination programs. This study provides a foundation for informed policymaking to enhance vaccine accessibility, inform targeted programs, and improve individual health systems, thereby addressing broader implications for global health.
Interaction of wheat cultivar and enzyme on broiler growth, nutrient utilization, and gut microflora
Authors: Seyedoshohadaei Seyedkamyar , Torki Mehran , Yaghoubfar Akbar , Abdolmohammadi Alireza
Published: November 2024

This study examined the impacts of four wheat cultivars and enzyme supplementation on growth performance, nutrient digestibility, and ileal microbiota composition in broiler chickens. Six hundred forty-eight male broilers (1-day-old, Ross 308) were studied in a completely randomized design factorial 4 × 2 along with control (9 treatments) with 6 replications (12 birds per pen). The Diets consisted of the four varieties of wheat (Sardari, Azar2, Sirvan, and Pishgam) with and without enzyme supplementation, alongside a corn-based control diet. All diets were iso-caloric and iso-nitrogenous. Daily weight gain (DWG) and feed conversion ratio (FCR) were not significantly affected by the dietary treatments. Broilers fed the corn-based diet displayed higher feed intake (FI) than those fed diets containing different wheat cultivars. Enzyme supplementation in wheat-based diets did not impact broiler growth performance. There was an interaction between enzyme and wheat type for protein, fat, calcium, and phosphorus digestibility. Ileal microbiota analysis revealed no significant changes in Lactobacillus and Escherichia coli populations across treatments. Conversely, Enterococcus and Bifidobacteria populations exhibited significant differences, with the Sirvan cultivar diet promoting the highest bacterial counts. It was concluded that different wheat cultivars could affect growth performance, nutrient digestibility, and ileum microbiota, and the beneficial effect of supplemental enzymes was only evident in certain variables and depended on the specific wheat variety.
Sarcopenia and frailty among older Chinese adults: Findings from the CHARLS study
Authors: Xu Weiwei , Cai Jiasheng , Liu Yichen , Li Lian , Ye Xiaomei , Wang Ping , Lu Jiaping , Akkaif Mohammed Ahmed , Zhang Min
Published: November 2024

Background

Sarcopenia and frailty are common among elderly individuals and present substantial health hazards. Exploring their relationship is essential for optimizing geriatric healthcare, particularly within the context of China.

Methods

A population-based cross-sectional design was employed using data from the China Health and Retirement Longitudinal Study (CHARLS). The study enrolled 5,714 participants aged ≥60 years who underwent assessment for sarcopenia following the criteria established by the Asia Working Group for Sarcopenia (AWGS 2019) in 2019. Frailty status was determined using a frailty index that categorized participants into robustness, pre-frailty, and frailty stages. Multivariable logistic regression models were employed to examine the relationship between sarcopenia and frailty and pre-frailty conditions. Subgroup and interaction analyses were performed to explore the robustness of the associations between sarcopenia and frailty across different subgroups.

Results

Among the participants, 1,028 (18.0%) were identified as frailty, 2,987 (52.3%) as pre-frailty, and 645 (11.3%) had sarcopenia. Sarcopenia demonstrated an independent association with higher risks of frailty (OR = 2.13, 95% CI: 1.52–2.99) and pre-frailty (OR = 1.42, 95% CI: 1.20–1.81) in the multivariable logistic analysis. Subgroup and interaction analyses consistently demonstrated significant associations across nearly all demographic and health-related subgroups.

Conclusions

This study highlights that sarcopenia is significantly and independently associated with frailty and pre-frailty among older adults in China. Early detection and targeted interventions for sarcopenia are crucial to mitigate frailty and its adverse health outcomes in aging populations, emphasizing the need for tailored healthcare strategies to promote healthy aging.