bluuebunny/crossref_metadata_2025 huggingface dataset:
Gravity Wave Breaking Associated with Mesospheric Inversion Layers as Measured by the Ship-Borne BEM Monge Lidar and ICON-MIGHTI
Authors: Wing Robin , Martic Milena , Triplett Colin , Hauchecorne Alain , Porteneuve Jacques , Keckhut Philippe , Courcoux Yann , Yung Laurent , Retailleau Patrick , Cocuron Dorothee
Published: October 2021

During a recent 2020 campaign, the Rayleigh lidar aboard the Bâtiment d’Essais et de Mesures (BEM) Monge conducted high-resolution temperature measurements of the upper Mesosphere and Lower Thermosphere (MLT). These measurements were used to conduct the first validation of ICON-MIGHTI temperatures by Rayleigh lidar. A double Mesospheric Inversion Layer (MIL) as well as shorter-period gravity waves was observed. Zonal and meridional wind speeds were obtained from locally launched radiosondes and the newly launched ICON satellite as well as from the European Centre for Medium-Range Weather Forecasts (ECMWF-ERA5) reanalysis. These three datasets allowed us to see the evolution of the winds in response to the forcing from the MIL and gravity waves. The wavelet analysis of a case study suggests that the wave energy was dissipated in small, intense, transient instabilities about a given wavenumber in addition to via a broad spectrum of breaking waves. This article will also detail the recent hardware advances of the Monge lidar that have allowed for the measurement of MILs and gravity waves at a resolution of 5 min with an effective vertical resolution of 926 m.
A Paleoclimate Prognosis of the Future Asian Summer Monsoon Variability
Authors: Tan Liangcheng , Li Yanzhen , Han Wenxia
Published: October 2021

In recent years, more and more record-breaking extreme weather/climate events have been reported from the Asian monsoon region, which have caused tremendous loss of property and lives. In this paper, we analyzed the Asian summer monsoon (ASM) variability during the Holocene and evaluated future climate extremes in monsoonal China from a paleoclimatic view. We found a significant regime transition to more chaotic fluctuations, with enhanced decadal variability of the ASM since 6.6 ka BP. We suggested the gradual intensification of ENSO was responsible for enhancing the ASM variability since the late mid-Holocene. If the observed relationship of monsoon mean intensity, ENSO and decadal variability of the ASM in the past 11.2 ka continue to exist, enhanced decadal variability of ASM in the future warming world will be expected. As a result, the intensification of daily precipitation extremes, superimposed on enhanced decadal variability of ASM, might make the record-breaking extremes more frequent in the future, increasing the risk of climate-related disasters in China.
Physiological and Yield Responses of Spring Wheat Cultivars under Realistic and Acute Levels of Ozone
Authors: Chaudhary Nivedita , Bonfil David J. , Tas Eran
Published: October 2021

Tropospheric ozone (O3) is widely recognized as the cause of substantial yield and quality reduction in crops. Most of the previous studies focused on the exposure of wheat cultivars to elevated O3 levels. Our main objectives were to: (i) investigate the consistency of wheat cultivars’ physiological responses across two different realistic O3 levels; and (ii) compare these physiological responses with those under short acute O3 exposure. Three commercially available hard spring wheat cultivars bred under semiarid and Eastern Mediterranean conditions were exposed to two different O3 levels during two consecutive seasons (2016–2018)—36 and 71 ppbv 7 h mean O3 mixing ratios in open-top chambers. The results were compared to those following short acute O3 exposure (102.8 ppbv, 7 h mean for 10 days) in a greenhouse. Non-stomatal responses were significantly more pronounced than stomatal responses in all cultivars under different levels of O3. The specific cultivar was observed as the most O3-tolerant under all experiments. The fact that the same cultivar was found remarkably tolerant to the local semiarid ambient conditions according to other studies and to O3 exposure based on the present study supports a link between cultivar resistance to drought conditions and O3.
On the Composition of Overlap and Grouping Functions
Authors: Dai Songsong , Du Lei , Song Haifeng , Xu Yingying
Published: October 2021

Obtaining overlap/grouping functions from a given pair of overlap/grouping functions is an important method of generating overlap/grouping functions, which can be viewed as a binary operation on the set of overlap/grouping functions. In this paper, firstly, we studied closures of overlap/grouping functions w.r.t. ⊛-composition. In addition, then, we show that these compositions are order preserving. Finally, we investigate the preservation of properties like idempotency, migrativity, homogeneity, k-Lipschitz, and power stable.
Abnormal Enhancement of Protein Disulfide Isomerase-like Activity of a Cyclic Diselenide Conjugated with a Basic Amino Acid by Inserting a Glycine Spacer
Authors: Mikami Rumi , Tsukagoshi Shunsuke , Arai Kenta
Published: October 2021

In a previous study, we reported that (S)-1,2-diselenane-4-amine (1) catalyzes oxidative protein folding through protein disulfide isomerase (PDI)-like catalytic mechanisms and that the direct conjugation of a basic amino acid (Xaa: His, Lys, or Arg) via an amide bond improves the catalytic activity of 1 by increasing its diselenide (Se–Se) reduction potential (E′°). In this study, to modulate the Se–Se redox properties and the association of the compounds with a protein substrate, new catalysts, in which a Gly spacer was inserted between 1 and Xaa, were synthesized. Exhaustive comparison of the PDI-like catalytic activities and E′° values among 1, 1-Xaa, and 1-Gly-Xaa showed that the insertion of a Gly spacer into 1-Xaa either did not change or slightly reduced the PDI-like activity and the E′° values. Importantly, however, only 1-Gly-Arg deviated from this generality and showed obviously increased E°′ value and PDI-like activity compared to the corresponding compound with no Gly spacer (1-Arg); on the contrary, its catalytic activity was the highest among the diselenide compounds employed in this study, while this abnormal enhancement of the catalytic activity of 1-Gly-Arg could not be fully explained by the thermodynamics of the Se–Se bond and its association ability with protein substrates.
The Parathyroid Hormone-Related Protein/Parathyroid Hormone 1 Receptor Axis in Adipose Tissue
Authors: Izquierdo-Lahuerta Adriana
Published: October 2021

Adipose tissue is an organ that shows great plasticity and is able to adapt to the conditions to which the body is subdued. It participates in the regulation of energetic homeostasis and has endocrine functions. Recent studies have shown how the parathyroid hormone-related protein (PTHrP)/Parathyroid Hormone Receptor 1 (PTH1R) axis participates in the regulation of adipogenesis, opposing the action of Peroxisome proliferator-activated receptor gamma (PPARγ). In addition to this, PTHrP is overexpressed in adipose tissue in situations of wear and tear of the body, favoring browning and lipolysis in this tissue. It is also overexpressed in adipose tissue in stressful situations but in the opposite direction, in obesity, metabolic syndrome, type 2 diabetes mellitus (T2DM) and gestational diabetes mellitus (GDM). In conclusion, the PTHrP/PTH1R axis has a main role in adipose tissue, participating in its differentiation and remodeling. PTHrP might be used in obesity treatment and its complications for its ability to reprogram adipogenesis and adipose tissue expansion, WAT browning and for the improvement of the insulin sensitivity. In addition, PTHrP could even be used as a marker of placental status and maternal adaptations to prevent future metabolic problems in mothers and children, as well as in the treatment of bone-related diseases such as osteoporosis.
Neuropsychological Sequelae, Quality of Life and Adaptive Behavior in Children and Adolescents with Anti-NMDAR Encephalitis: A Narrative Review
Authors: Tarantino Samuela , Averna Roberto , Ruscitto Claudia , Ursitti Fabiana , Ferilli Michela Ada Noris , Moavero Romina , Papetti Laura , Proietti Checchi Martina , Sforza Giorgia , Balestri Martina , Grimaldi Capitello Teresa , Vigevano Federico , Vicari Stefano , Valeriani Massimiliano
Published: October 2021

Anti-N-methyl-d-aspartate receptor (anti-NMDAR) encephalitis is a rare autoimmune illness characterized by a constellation of often severe, but treatable, psychiatric and neurological symptoms. Whereas symptoms such as psychosis and bizarre and abnormal motor behavior are common in adults, pediatric patients typically present with behavioral changes, irritability and sleep dysfunction. The recovery phase is usually slow and may be associated with longstanding adaptive, behavioral and neuropsychological problems. Very few studies explored the cognitive and adaptive sequelae in children with anti-NMDAR encephalitis. The present review article suggests that, although most children and adolescents return to their daily life and previous activities, they may have a low quality of life and show neuropsychological sequelae involving language, memory, especially verbal memory, and attentional resources, even after several months from the hospital discharge. In particular, the available results reveal difficulties in cognitive skills involving executive functions. This impairment is considered the “core” of the cognitive profile of young patients with anti-NMDAR encephalitis. On the other hand, some cognitive skills, such as general intelligence, show good overall recovery over time. Additional neuropsychological research evaluating larger samples, more homogenous methods and longitudinal studies is required.
Manifold Feature Fusion with Dynamical Feature Selection for Cross-Subject Emotion Recognition
Authors: Hua Yue , Zhong Xiaolong , Zhang Bingxue , Yin Zhong , Zhang Jianhua
Published: October 2021

Affective computing systems can decode cortical activities to facilitate emotional human–computer interaction. However, personalities exist in neurophysiological responses among different users of the brain–computer interface leads to a difficulty for designing a generic emotion recognizer that is adaptable to a novel individual. It thus brings an obstacle to achieve cross-subject emotion recognition (ER). To tackle this issue, in this study we propose a novel feature selection method, manifold feature fusion and dynamical feature selection (MF-DFS), under transfer learning principle to determine generalizable features that are stably sensitive to emotional variations. The MF-DFS framework takes the advantages of local geometrical information feature selection, domain adaptation based manifold learning, and dynamical feature selection to enhance the accuracy of the ER system. Based on three public databases, DEAP, MAHNOB-HCI and SEED, the performance of the MF-DFS is validated according to the leave-one-subject-out paradigm under two types of electroencephalography features. By defining three emotional classes of each affective dimension, the accuracy of the MF-DFS-based ER classifier is achieved at 0.50–0.48 (DEAP) and 0.46–0.50 (MAHNOBHCI) for arousal and valence emotional dimensions, respectively. For the SEED database, it achieves 0.40 for the valence dimension. The corresponding accuracy is significantly superior to several classical feature selection methods on multiple machine learning models.
Systematic Review on Intra- and Extracochlear Electrical Stimulation for Tinnitus
Authors: Assouly Kelly K. S. , Dullaart Max J. , Stokroos Robert J. , van Dijk Bas , Stegeman Inge , Smit Adriana L.
Published: October 2021

Several electrical stimulation patterns of the auditory nerve have been described for tinnitus relief, but there is no consensus on the most effective stimulation pattern. Therefore, we aim to systematically review the literature on the effect of intra- and extracochlear electrical stimulation patterns as a treatment option for patients with tinnitus. Only studies on intra- and extracochlear electrical stimulation for patients with tinnitus were included if the stimulation used did not concern standardized CI stimulation patterns to primarily rehabilitate hearing loss as intervention. A total of 34 studies met the inclusion criteria, with 10 studies (89 patients) on intracochlear electrical stimulation and 25 studies on extracochlear electrical stimulation (1109 patients). There was a high to medium risk of bias in 22 studies, especially due to lack of a non-exposed group and poor selection of the exposed group. All included studies showed subjective tinnitus improvement during or after electrical stimulation, using different stimulation patterns. Due to methodological limitations and low reporting quality of the included studies, the potential of intra- and extracochlear stimulation has not been fully explored. To draw conclusions on which stimulation patterns should be optimized for tinnitus relief, a deeper understanding of the mechanisms involved in tinnitus suppression is needed.
Biological Bases of Empathy and Social Cognition in Patients with Attention-Deficit/Hyperactivity Disorder: A Focus on Treatment with Psychostimulants
Authors: Fantozzi Pamela , Sesso Gianluca , Muratori Pietro , Milone Annarita , Masi Gabriele
Published: October 2021

In recent years, there has been growing interest in investigating the effect of specific pharmacological treatments for ADHD not only on its core symptoms, but also on social skills in youths. This stands especially true for ADHD patients displaying impulsive aggressiveness and antisocial behaviors, being the comorbidity with Disruptive Behavior Disorders, one of the most frequently observed in clinical settings. This systematic review aimed to synthesize research findings on this topic following PRISMA guidelines and to identify gaps in current knowledge, future directions, and treatment implications. Search strategies included the following terms: ADHD; methylphenidate and other ADHD drugs; empathy, theory of mind and emotion recognition. Full-text articles were retrieved and data from individual studies were collected. Thirteen studies were finally included in our systematic review. Ten studies assessing changes in empathy and/or theory of mind in patients with ADHD treated after pharmacological interventions were identified. Similarly, seven partially overlapping studies assessing changes in emotion recognition were retrieved. Despite a great heterogeneity in the methodological characteristics of the included studies, most of them reported an improvement in emphatic and theory of mind abilities in youths with ADHD treated with psychostimulants and nonstimulant drugs, as well as positive but less consistent results about emotion recognition performances.
The Influence of Intraocular Lens Implantation and Alterations in Blue Light Transmittance Level on the Brain Functional Network Architecture Reorganization in Cataract Patients
Authors: Sobczak Anna Maria , Bohaterewicz Bartosz , Fafrowicz Magdalena , Domagalik Aleksandra , Beldzik Ewa , Oginska Halszka , Golonka Natalia , Rekas Marek , Bronicki Dominik , Romanowska-Dixon Bożena , Bolsega-Pacud Joanna , Karwowski Waldemar , Farahani Farzad V. , Marek Tadeusz
Published: October 2021

Background: Cataract is one of the most common age-related vision deteriorations, leading to opacification of the lens and therefore visual impairment as well as blindness. Both cataract extraction and the implantation of blue light filtering lens are believed to improve not only vision but also overall functioning. Methods: Thirty-four cataract patients were subject to resting-state functional magnetic resonance imaging before and after cataract extraction and intraocular lens implantation (IOL). Global and local graph metrics were calculated in order to investigate the reorganization of functional network architecture associated with alterations in blue light transmittance. Psychomotor vigilance task (PVT) was conducted. Results: Graph theory-based analysis revealed decreased eigenvector centrality after the cataract extraction and IOL replacement in inferior occipital gyrus, superior parietal gyrus and many cerebellum regions as well as increased clustering coefficient in superior and inferior parietal gyrus, middle temporal gyrus and various cerebellum regions. PVT results revealed significant change between experimental sessions as patients responded faster after IOL replacement. Moreover, a few regions were correlated with the difference in blue light transmittance and the time reaction in PVT. Conclusion: Current study revealed substantial functional network architecture reorganization associated with cataract extraction and alteration in blue light transmittance.
Balance Expertise Is Associated with Superior Spatial Perspective-Taking Skills
Authors: Hötting Kirsten , Rogge Ann-Kathrin , Kuhne Laura A. , Röder Brigitte
Published: October 2021

Balance training interventions over several months have been shown to improve spatial cognitive functions and to induce structural plasticity in brain regions associated with visual-vestibular self-motion processing. In the present cross-sectional study, we tested whether long-term balance practice is associated with better spatial cognition. To this end, spatial perspective-taking abilities were compared between balance experts (n = 40) practicing sports such as gymnastics, acrobatics or slacklining for at least four hours a week for the last two years, endurance athletes (n = 38) and sedentary healthy individuals (n = 58). The balance group showed better performance in a dynamic balance task compared to both the endurance group and the sedentary group. Furthermore, the balance group outperformed the sedentary group in a spatial perspective-taking task. A regression analysis across all participants revealed a positive association between individual balance performance and spatial perspective-taking abilities. Groups did not differ in executive functions, and individual balance performance did not correlate with executive functions, suggesting a specific association between balance skills and spatial cognition. The results are in line with theories of embodied cognition, assuming that sensorimotor experience shapes cognitive functions.
The Utility of Differential Scanning Calorimetry Curves of Blood Plasma for Diagnosis, Subtype Differentiation and Predicted Survival in Lung Cancer
Authors: Schneider Gabriela , Kaliappan Alagammai , Nguyen Taylor Q. , Buscaglia Robert , Brock Guy N. , Hall Melissa Barousse , DeSpirito Crissie , Wilkey Daniel W. , Merchant Michael L. , Klein Jon B. , Wiese Tanya A. , Rivas-Perez Hiram L. , Kloecker Goetz H. , Garbett Nichola C.
Published: October 2021

Early detection of lung cancer (LC) significantly increases the likelihood of successful treatment and improves LC survival rates. Currently, screening (mainly low-dose CT scans) is recommended for individuals at high risk. However, the recent increase in the number of LC cases unrelated to the well-known risk factors, and the high false-positive rate of low-dose CT, indicate a need to develop new, non-invasive methods for LC detection. Therefore, we evaluated the use of differential scanning calorimetry (DSC) for LC patients’ diagnosis and predicted survival. Additionally, by applying mass spectrometry, we investigated whether changes in O- and N-glycosylation of plasma proteins could be an underlying mechanism responsible for observed differences in DSC curves of LC and control subjects. Our results indicate selected DSC curve features could be useful for differentiation of LC patients from controls with some capable of distinction between subtypes and stages of LC. DSC curve features also correlate with LC patients’ overall/progression free survival. Moreover, the development of classification models combining patients’ DSC curves with selected plasma protein glycosylation levels that changed in the presence of LC could improve the sensitivity and specificity of the detection of LC. With further optimization and development of the classification method, DSC could provide an accurate, non-invasive, radiation-free strategy for LC screening and diagnosis.
A Multi-Modal Toolkit for Studying Neutrophils in Cancer and Beyond
Authors: Changirwa Diana , Schlechte Jared , McDonald Braedon
Published: October 2021

As key effector cells of the innate immune response, neutrophils are rapidly deployed to sites of inflammation where they deliver a payload of potent effector mechanisms that are essential for host defense against pathogens as well as tissue homeostasis. In addition, neutrophils are central contributors to the pathogenesis of a vast spectrum of inflammatory, degenerative, and neoplastic diseases. As our understanding of neutrophils in health and disease continually expands, so too does our appreciation of their complex and dynamic nature in vivo; from development, maturation, and trafficking to cellular heterogeneity and functional plasticity. Therefore, contemporary neutrophil research relies on multiple complementary methodologies to perform integrated analysis of neutrophil phenotypic heterogeneity, organ- and stimulus-specific trafficking mechanisms, as well as tailored effector functions in vivo. This review discusses established and emerging technologies used to study neutrophils, with a focus on in vivo imaging in animal models, as well as next-generation ex vivo model systems to study mechanisms of neutrophil function. Furthermore, we discuss how high-dimensional single-cell analysis technologies are driving a renaissance in neutrophil biology by redefining our understanding of neutrophil development, heterogeneity, and functional plasticity. Finally, we discuss innovative applications and emerging opportunities to integrate these high-dimensional, multi-modal techniques to deepen our understanding of neutrophils in cancer research and beyond.
Ambient Air Purification by Nanotechnologies: From Theory to Application
Authors: Cao Jun-ji , Huang Yu , Zhang Qian
Published: October 2021

Air pollution has been a recurring problem in northern Chinese cities, and high concentrations of PM2.5 in winter have been a particular cause for concern. Secondary aerosols converted from precursor gases (i.e., nitrogen oxides and volatile organic compounds) evidently account for a large fraction of the PM2.5. Conventional control methods, such as dust removal, desulfurization, and denitrification, help reduce emissions from stationary combustion sources, but these measures have not led to decreases in haze events. Recent advances in nanomaterials and nanotechnology provide new opportunities for removing fine particles and gaseous pollutants from ambient air and reducing the impacts on human health. This review begins with overviews of air pollution and traditional abatement technologies, and then advances in ambient air purification by nanotechnologies, including filtration, adsorption, photocatalysis, and ambient-temperature catalysis are presented—from fundamental principles to applications. Current state-of-the-art developments in the use of nanomaterials for particle removal, gas adsorption, and catalysis are summarized, and practical applications of catalysis-based techniques for air purification by nanomaterials in indoor, semi-enclosed, and open spaces are highlighted. Finally, we propose future directions for the development of novel disinfectant nanomaterials and the construction of advanced air purification devices.
Enhanced In Vitro Cascade Catalysis of Glycerol into Pyruvate and Acetoin by Integration with Dihydroxy Acid Dehydratase from Paralcaligenes ureilyticus
Authors: Guo Shiting , Tan Xiaoxu , Wang Yuxian , Li Kai , Lü Chuanjuan , Ma Cuiqing , Gao Chao
Published: October 2021

Recently, an in vitro enzymatic cascade was constructed to transform glycerol into the high-value platform chemical pyruvate. However, the low activity of dihydroxy acid dehydratase from Sulfolobus solfataricus (SsDHAD) limited the efficiency. In this study, the enzymatic reduction of pyruvate catalyzed by d-lactate dehydrogenase from Pseudomonas aeruginosa PAO1 was used to assay the activities of dihydroxy acid dehydratases. Dihydroxy acid dehydratase from Paralcaligenes ureilyticus (PuDHT) was identified as the most efficient candidate for glycerate dehydration. After the optimization of the catalytic temperature for the enzymatic cascade, comprising alditol oxidase from Streptomyces coelicolor A3, PuDHT, and catalase from Aspergillus niger, 20.50 ± 0.27 mM of glycerol was consumed in 4 h to produce 18.95 ± 0.97 mM of pyruvate with a productivity 12.15-fold higher than the previous report using SsDHAD. The enzymatic cascade was further coupled with the pyruvate decarboxylase from Zymomonas mobile for the production of another platform compound, acetoin. Acetoin at a concentration of 8.52 ± 0.12 mM was produced from 21.62 ± 0.19 mM of glycerol with a productivity of 1.42 ± 0.02 mM h−1.
High Immunoproteasome Activity and sXBP1 in Pediatric Precursor B-ALL Predicts Sensitivity towards Proteasome Inhibitors
Authors: Besse Lenka , Besse Andrej , Kraus Marianne , Maurits Elmer , Overkleeft Herman S. , Bornhauser Beat , Bourquin Jean-Pierre , Driessen Christoph
Published: October 2021

Proteasome inhibitors (PIs) are approved backbone treatments in multiple myeloma. More recently, inhibition of proteasome activity with the PI bortezomib has been clinically evaluated as a novel treatment strategy in pediatric acute lymphoblastic leukemia (ALL). However, we lack a marker that could identify ALL patients responding to PI-based therapy. By using a set of activity-based proteasome probes in conjunction with cytotoxicity assays, we show that B-cell precursor ALL (BCP-ALL), in contrast to T-ALL, demonstrates an increased activity of immunoproteasome over constitutive proteasome, which correlates with high ex vivo sensitivity to the PIs bortezomib and ixazomib. The novel selective PI LU015i-targeting immunoproteasome β5i induces cytotoxicity in BCP-ALL containing high β5i activity, confirming immunoproteasome activity as a novel therapeutic target in BCP-ALL. At the same time, cotreatment with β2-selective proteasome inhibitors can sensitize T-ALL to currently available PIs, as well as to β5i selective PI. In addition, levels of total and spliced forms of XBP1 differ between BCP-ALL and T-ALL, and only in BCP-ALL does high-spliced XBP1 correlate with sensitivity to bortezomib. Thus, in BCP-ALL, high immunoproteasome activity may serve as a predictive marker for PI-based treatment options, potentially combined with XBP1 analyses.
Silencing of the ER and Integrative Stress Responses in the Liver of Mice with Error-Prone Translation
Authors: Moore James , Osinnii Ivan , Grimm Amandine , Oettinghaus Björn , Eckert Anne , Frank Stephan , Böttger Erik C.
Published: October 2021

Translational errors frequently arise during protein synthesis, producing misfolded and dysfunctional proteins. Chronic stress resulting from translation errors may be particularly relevant in tissues that must synthesize and secrete large amounts of secretory proteins. Here, we studied the proteostasis networks in the liver of mice that express the Rps2-A226Y ribosomal ambiguity (ram) mutation to increase the translation error rate across all proteins. We found that Rps2-A226Y mice lack activation of the eIF2 kinase/ATF4 pathway, the main component of the integrated stress response (ISR), as well as the IRE1 and ATF6 pathways of the ER unfolded protein response (ER-UPR). Instead, we found downregulation of chronic ER stress responses, as indicated by reduced gene expression for lipogenic pathways and acute phase proteins, possibly via upregulation of Sirtuin-1. In parallel, we observed activation of alternative proteostasis responses, including the proteasome and the formation of stress granules. Together, our results point to a concerted response to error-prone translation to alleviate ER stress in favor of activating alternative proteostasis mechanisms, most likely to avoid cell damage and apoptotic pathways, which would result from persistent activation of the ER and integrated stress responses.
Paracrine Regulation of Alveolar Epithelial Damage and Repair Responses by Human Lung-Resident Mesenchymal Stromal Cells
Authors: Kruk Dennis M. L. W. , Wisman Marissa , Noordhoek Jacobien A. , Nizamoglu Mehmet , Jonker Marnix R. , de Bruin Harold G. , Arevalo Gomez Karla , ten Hacken Nick H. T. , Pouwels Simon D. , Heijink Irene H.
Published: October 2021

COPD is characterized by irreversible lung tissue damage. We hypothesized that lung-derived mesenchymal stromal cells (LMSCs) reduce alveolar epithelial damage via paracrine processes, and may thus be suitable for cell-based strategies in COPD. We aimed to assess whether COPD-derived LMSCs display abnormalities. LMSCs were isolated from lung tissue of severe COPD patients and non-COPD controls. Effects of LMSC conditioned-medium (CM) on H2O2-induced, electric field- and scratch-injury were studied in A549 and NCI-H441 epithelial cells. In organoid models, LMSCs were co-cultured with NCI-H441 or primary lung cells. Organoid number, size and expression of alveolar type II markers were assessed. Pre-treatment with LMSC-CM significantly attenuated oxidative stress-induced necrosis and accelerated wound repair in A549. Co-culture with LMSCs supported organoid formation in NCI-H441 and primary epithelial cells, resulting in significantly larger organoids with lower type II-marker positivity in the presence of COPD-derived versus control LMSCs. Similar abnormalities developed in organoids from COPD compared to control-derived lung cells, with significantly larger organoids. Collectively, this indicates that LMSCs’ secretome attenuates alveolar epithelial injury and supports epithelial repair. Additionally, LMSCs promote generation of alveolar organoids, with abnormalities in the supportive effects of COPD-derived LMCS, reflective of impaired regenerative responses of COPD distal lung cells.
A-Kinase Anchoring Protein 2 Promotes Protection against Myocardial Infarction
Authors: Maric Darko , Paterek Aleksandra , Delaunay Marion , López Irene Pérez , Arambasic Miroslav , Diviani Dario
Published: October 2021

Myocardial infarction (MI) is a leading cause of maladaptive cardiac remodeling and heart failure. In the damaged heart, loss of function is mainly due to cardiomyocyte death and remodeling of the cardiac tissue. The current study shows that A-kinase anchoring protein 2 (AKAP2) orchestrates cellular processes favoring cardioprotection in infarcted hearts. Induction of AKAP2 knockout (KO) in cardiomyocytes of adult mice increases infarct size and exacerbates cardiac dysfunction after MI, as visualized by increased left ventricular dilation and reduced fractional shortening and ejection fraction. In cardiomyocytes, AKAP2 forms a signaling complex with PKA and the steroid receptor co-activator 3 (Src3). Upon activation of cAMP signaling, the AKAP2/PKA/Src3 complex favors PKA-mediated phosphorylation and activation of estrogen receptor α (ERα). This results in the upregulation of ER-dependent genes involved in protection against apoptosis and angiogenesis, including Bcl2 and the vascular endothelial growth factor a (VEGFa). In line with these findings, cardiomyocyte-specific AKAP2 KO reduces Bcl2 and VEGFa expression, increases myocardial apoptosis and impairs the formation of new blood vessels in infarcted hearts. Collectively, our findings suggest that AKAP2 organizes a transcriptional complex that mediates pro-angiogenic and anti-apoptotic responses that protect infarcted hearts.
RASSF1A-Mediated Suppression of Estrogen Receptor Alpha (ERα)-Driven Breast Cancer Cell Growth Depends on the Hippo-Kinases LATS1 and 2
Authors: Roßwag Sven , Sleeman Jonathan P. , Thaler Sonja
Published: October 2021

Around 70% of breast cancers express the estrogen receptor alpha (ERα). This receptor is of central importance for breast cancer development and estrogen-dependent tumor growth. However, the molecular mechanisms that are responsible for the control of ERα expression and function in the context of breast carcinogenesis are complex and not fully understood. In previous work, we have demonstrated that the tumor suppressor RASSF1A suppresses estrogen-dependent growth of breast cancer cells through a complex network that keeps ERα expression and function under control. We observed that RASSF1A mediates the suppression of ERα expression through modulation of the Hippo effector Yes-associated protein 1 (YAP1) activity. Here we report that RASSF1A-mediated alteration of YAP1 depends on the Hippo-kinases LATS1 and LATS2. Based on these results, we conclude that inactivation of RASSF1A causes changes in the function of the Hippo signaling pathway and altered activation of YAP1, and as a consequence, increased expression and function of ERα. Thus, the inactivation of RASSF1A might constitute a fundamental event that supports the initiation of ERα-dependent breast cancer. Furthermore, our results support the notion that the Hippo pathway is important for the suppression of luminal breast cancers, and that the tumor-suppressor function of RASSF1A depends on LATS1 and LATS2.
Oral Health-Related Quality of Life (OHRQoL) of Children with Down Syndrome and Their Families: A Cross-Sectional Study
Authors: AlJameel AlBandary Hassan , AlKawari Huda
Published: October 2021

As individuals with Down syndrome often suffer from oro-facial abnormalities which can affect their oral health as well as their and their family’s quality of life, this link was examined in the present study. Using a descriptive cross-sectional design, 63 parents of children with Down syndrome who attended two special daycare centres in Riyadh, Saudi Arabia, were surveyed using a self-administered validated questionnaire. The findings yielded by the Statistical Package for the Social Sciences (SPSS Inc., Chicago, IL, USA) version 17 revealed that in 34.9% children and 46% of their families, quality of life was affected by oral health. Moreover, 54% children experienced physical pain, which was severe in 22.2% of the cases. Further analyses revealed that families’ emotional lives were negatively affected by children’s oral health status. Therefore, as oral health in children with Down syndrome exerts significant adverse impacts on different aspects of their lives and those of their families, timely provision of required oral health care is warranted.
Preparation of Wear-Resistant Coating on Ti6Al4V Alloy by Cold Spraying and Plasma Electrolytic Oxidation
Authors: Shao Mingzeng , Wang Wei , Yang Hongbo , Zhang Xueer , He Xiaomei
Published: October 2021

In order to improve the wear resistance of Ti6Al4V alloy, the alloy was first coated with alumina-reinforced aluminum coating (CS-coating) by cold spraying, and then the alloy with CS-coating was processed by plasma electrolytic oxidation (PEO) under unipolar mode and soft sparking mode, respectively, to prepare wear-resistant PEO coatings. For comparison, Ti6Al4V alloy without CS-coating was also subjected to PEO treatment. The microstructure, phase composition, hardness, and wear resistance of the PEO coatings formed on Ti6Al4V alloy with and without CS-coating were investigated. The results revealed that PEO coatings formed on Ti6Al4V alloy with CS-coating under soft sparking mode contained more α-Al2O3, possessed larger thickness, more compact microstructure, and higher microhardness than that formed under unipolar mode. The PEO coating formed on Ti6Al4V substrate was mainly composed of TiO2 and had pores and cracks. Among all these coatings, PEO coating formed on Ti6Al4V alloy with CS-coating under soft sparking mode exhibited the best wear resistance with a wear rate of 1.18 × 10−5 mm3/(Nm), which was only 15.28% of that of the Ti6Al4V substrate. The investigation indicated that the combination of cold spraying and PEO under soft sparking mode is a promising technique for improving the wear resistance of titanium alloy.
Annealing Studies of Copper Indium Oxide (Cu2In2O5) Thin Films Prepared by RF Magnetron Sputtering
Authors: Skaria Giji , Saikumar Ashwin Kumar , Shivprasad Akshaya D. , Sundaram Kalpathy B.
Published: October 2021

Copper indium oxide (Cu2In2O5) thin films were deposited by the RF magnetron sputtering technique using a Cu2O:In2O3 target. The films were deposited on glass and quartz substrates at room temperature. The films were subsequently annealed at temperatures ranging from 100 to 900 °C in an O2 atmosphere. The X-ray diffraction (XRD) analysis performed on the samples identified the presence of Cu2In2O5 phases along with CuInO2 or In2O3 for the films annealed above 500 °C. An increase in grain size was identified with the increase in annealing temperatures from the XRD analysis. The grain sizes were calculated to vary between 10 and 27 nm in films annealed between 500 and 900 °C. A morphological study performed using SEM further confirmed the crystallization and the grain growth with increasing annealing temperatures. All films displayed high optical transmission of more than 70% in the wavelength region of 500–800 nm. Optical studies carried out on the films indicated a small bandgap change in the range of 3.4–3.6 eV during annealing.
Tunable Narrow-Linewidth Ring Laser Based on the Polarization Conversion in a Tapered Fiber-Coupled Whispering Gallery Mode Resonator
Authors: Chang Pengfa , Wang Chen , Han Hong , Gao Feng , Huang Ligang , Wang Anbang
Published: October 2021

Based on the polarization conversion in a tapered fiber-coupled whispering gallery mode (WGM) system, a simple-structured narrow linewidth band-pass filter was fabricated and a narrow linewidth ring laser was demonstrated. With a fiber Bragg grating (FBG) to further select the resonant mode, the ring laser could be in the single-longitudinal-mode with the linewidth about 6 kHz and its wavelength could be tuned from 1540.10 nm to 1570.01 nm. Benefit from the dense modes in the WGM resonator, high conversion efficiency, and better stability of the structure, a tunable dual-wavelength laser could be achieved based on two FBGs with different resonant wavelengths. The configuration of the proposed laser is simple and stable, which will benefit its applications in the future.
Photodetector Based on CsPbBr3/Cs4PbBr6 Composite Nanocrystals with High Detectivity
Authors: Han Yue , Wen Rong , Zhang Feng , Shi Linlin , Wang Wenyan , Ji Ting , Li Guohui , Hao Yuying , Feng Lin , Cui Yanxia
Published: October 2021

High-quality, all-inorganic CsPbBr3/Cs4PbBr6 composite perovskite nanocrystals (NCs) were obtained with all-solution-processing at room temperature, and a photodetector (PD) with high detectivity was realized based on CsPbBr3/Cs4PbBr6 NCs. The detectivity (D*) of the proposed PD is 4.24 × 1012 Jones under 532 nm illumination, which is among the highest levels for PDs based on all-inorganic perovskite NCs. In addition, a high linear dynamic range (LDR) of 115 dB under 1 V bias was also realized. Furthermore, the underlying mechanism for the enhanced performance of the proposed PD was discussed. Our work might promote the preparation of high-performance PDs based on dual-phase all-inorganic perovskite nanocrystals.
Prostate Cancer Metastasis to the Pituitary Gland Manifesting as Corticosteroid Withdrawal, and the Impact of the Switch from Prednisone to Dexamethasone on Survival Time
Authors: Ataikiru Okeroghene , Abdelsalam Mahmoud , Avileli Mrudula , Hynes Trina
Published: October 2021

Despite improvements in the diagnosis and treatment of cancers, the incidence of pituitary metastasis has increased. Prostate cancer metastasis to the pituitary, however, is rare, and these tumors usually grow rapidly. They are also more likely to be located in the posterior pituitary, and the presenting symptoms are often nonspecific, which makes early diagnosis challenging. The management of this condition is usually multidisciplinary, and requires careful assessment and decision making. We present a case of a patient who developed prostate cancer metastasis to the pituitary. In this report, we show that patients with prostate cancer on corticosteroid therapy who develop withdrawal symptoms or other endocrine symptoms should be assessed for pituitary and other brain metastasis. This case report also discusses the impact of switching from prednisone and abiraterone to dexamethasone and abiraterone. Our report shows that patients on abiraterone and prednisone whose PSA has increased, but who have no radiologic progression, may have their PSA controlled and thereby improved survival time when they are switched to abiraterone and dexamethasone.
Shell Anomalies in the European Aquatic Stem Turtle Pleurosternon bullockii (Paracryptodira, Pleurosternidae)
Authors: Guerrero Andrea , Pérez-García Adán
Published: October 2021

The uppermost Jurassic to lowermost Cretaceous stem turtle Pleurosternon bullockii is the pleurosternid (Testudinata, Paracryptodira) known by the largest number of specimens worldwide, composing the largest European collection of Lower Cretaceous complete and partial shells for a turtle taxon. The availability of numerous specimens as well as their generally good preservation allowed for recent detailed characterization of the shell of this species, including states that are variable at the intraspecific level (individual variability, sexual dimorphism, and ontogenetic development). However, extreme cases of morphological variation corresponding to anomalies have not been addressed in detail, neither for P. bullockii nor for any other member of Paracryptodira. In this context, the study of several shell anomalies in P. bullockii is carried out here. Fourteen specimens showing anomalies are recognized and examined here to determine the frequency and distribution of these shell anatomical deviations. All these anomalies are described and figured. The morphogenetic cause of each of them is discussed. As a consequence, a relatively broad spectrum of anomalies is reported for P. bullockii. None of the anomalies seem to present negative consequences for vital activities of the specimens since none compromised the main functions of the shell.
Five Independent Lineages Revealed by Integrative Taxonomy in the Dendropsophus nanus–Dendropsophus walfordi Species Complex
Authors: Seger Karin Regina , da Veiga Teixeira Bernardo Franco , Annibale Fabiane Santana , Rossa-Feres Denise de Cerqueira , Lima Albertina Pimentel , Andrade Gilda Vasconcellos , Giaretta Ariovaldo Antonio , Lourenço Luciana Bolsoni
Published: October 2021

One of the many taxonomic challenges found in the Dendropsophus microcephalus species group is the Dendropsophus walfordi distinction from D. nanus. Recent phylogenetic inferences have indicated the paraphyly of these species, although they were not designed to assess this issue. To contribute to the delimitation of these species, we analyzed the 12S, 16S and COI mitochondrial genes, the morphological traits, and the advertisement calls of specimens from northern Amazonia to Argentina, including the type localities of D. nanus and D. walfordi. Paraphyly of D. nanus with respect to D. walfordi was inferred by maximum-parsimony and Bayesian analyses, and five major clades exhibiting nonoverlapping geographic distributions were recognized. The bPTP and ABGD analyses supported the existence of five independently evolving lineages in this complex. Acoustic and morphological data clearly distinguished the clade that included the topotypes of D. walfordi from the others, corroborating the validity of this species. To avoid the paraphyly of D. nanus with respect to D. walfordi, we recognize the clade distributed from central-southern Brazil to Argentina as D. nanus, the clade distributed in Amazonia as D. walfordi, and discuss the existence of unnamed cryptic species closely related to D. nanus and D. walfordi.
Aquatic Habitat Bird Occurrences at Photovoltaic Solar Energy Development in Southern California, USA
Authors: Kosciuch Karl , Riser-Espinoza Daniel , Moqtaderi Cyrus , Erickson Wallace
Published: October 2021

The development of photovoltaic (PV) utility-scale solar energy (USSE) in the desert Southwest has the potential to negatively affect birds through collision mortality. Based on early patterns in fatality monitoring data, the lake effect hypothesis (LEH) was developed and suggested that birds misinterpret PV solar panels for water. As the LEH was only recently defined and inference beyond bird mortality is limited, our research objective was to examine the species composition, abundance, and distribution of live and dead aquatic habitat birds at five PV solar facilities and paired reference areas in southern California. Further, we collected data from a small regional lake as an indicator of the potential aquatic habitat bird community that could occur at our study sites. Using an ordination analysis, we found the lake grouped away from the other study sites. Although the bird community (live and dead) at the solar facilities contained aquatic habitat species, Chao’s diversity was higher, and standardized use was more than an order of magnitude higher at the lake. Finally, we did not observe aquatic habitat bird fatalities in the desert/scrub and grassland reference areas. Thus, the idea of a “lake effect” in which aquatic habitat birds perceive a PV USSE facility as a waterbody and are broadly attracted is likely a nuanced process as a PV solar facility is unlikely to provide a signal of a lake to all aquatic habitat birds at all times.
Developing Inside a Layer of Germs—A Potential Role for Multiciliated Surface Cells in Vertebrate Embryos
Authors: Kerney Ryan
Published: October 2021

This paper reviews current research on the microbial life that surrounds vertebrate embryos. Several clades are believed to develop inside sterile—or near-sterile—embryonic microhabitats, while others thrive within a veritable zoo of microbial life. The occurrence of embryo-associated microbes in some groups, but not others, is an under-appreciated transition (possibly transitions) in vertebrate evolution. A lack of comparable studies makes it currently impossible to correlate embryo-associated microbiomes with other aspects of vertebrate evolution. However, there are embryonic features that should instruct a more targeted survey. This paper concludes with a hypothesis for the role of multiciliated surface cells in amphibian and some fish embryos, which may contribute to managing embryo-associated microbial consortia. These cells are known to exist in some species that harbor in ovo microbes or have relatively porous egg capsules, although most have not been assayed for embryo-associated microbiota. Whether the currents generated within these extraembryonic microhabitats contribute to culturing consistent microbial communities remains to be seen.
Texture and Color Enhancement Imaging Increases Color Changes and Improves Visibility for Squamous Cell Carcinoma Suspicious Lesions in the Pharynx and Esophagus
Authors: Dobashi Akira , Ono Shingo , Furuhashi Hiroto , Futakuchi Toshiki , Tamai Naoto , Yamauchi Takashi , Suka Machi , Sumiyama Kazuki
Published: October 2021

Texture and color enhancement imaging (TXI) has been developed as an image-enhanced endoscopy technology. TXI mode2 enhances texture and brightness, and TXI mode1 also enhances color. This study aims to assess the color differences in squamous cell carcinoma (SCC) suspicious lesions in the pharynx and esophagus using white light imaging (WLI), TXI mode1, TXI mode2, and narrow-band imaging (NBI). A total of 59 SCC suspicious lesions from 30 patients were analyzed. The color differences (ΔE) between the lesion and the surrounding mucosa were calculated for each modality. The color value was assessed using the Commission Internationale d’Eclairage L*a*b* color space. The visibility of the lesion in each modality was evaluated and compared to that in the WLI by six endoscopists. The mean ΔE values in the WLI, TXI mode1, TXI mode2, and NBI were 11.6; 18.6; 14.3; and 17.2, respectively, and the ΔE values of TXI mode1, TXI mode2, and NBI were significantly higher than those of the WLI (p < 0.001). No lesions had worse visibility, and 62.5% (37/59) had improved visibility, as assessed by more than half of the endoscopists in TXI mode1. TXI mode1 can enhance color changes and improve the visibility of SCC suspicious lesions in the pharynx and esophagus, compared to WLI.