bluuebunny/crossref_metadata_2025 huggingface dataset:
ON THE THRESHOLD: CREATIVITY IN MID-LIFE
Authors: Adams-Price Carolyn , O'Neill Desmond
Published: December 2022

While late-life creativity has attracted significant scholarly inquiry, it is not clear that the same focus has applied to creativity in midlife and its relationship to creativity across the lifespan. Allied to a proposed symphonic concert by the Indianapolis Symphony of works from late midlife creativity with gerontologically-themed pre-concert talks occurring alongside GSA 2022, an activity curated by the Humanities, Arts and Cultural Gerontology Advisory Panel of GSA, this symposium will provide insights into a relatively under-explored aspect of creativity and aging, that of midlife creativity and its relationship to creativity at other stages of life. For many artists the most significant breakthroughs in style and critical reception are found in the works of midlife, and the symposium will explore the phenomenology and underlying themes of midlife creativity. This will include a review of the literature, a focus on midlife breakthrough and development in the creativity of Brahms and Janacek, interaction with the program of the parallel symphony concert, and a focus on arts and crafts creativity in people newly undertaking arts and crafts in mid-life. This will be complemented by a review of creativity and well-being from the age of 50 onwards.
RACIAL DISPARITIES IN THE ASSOCIATION BETWEEN VISION IMPAIRMENT AND FOOD INSECURITY IN THE US
Authors: Varadaraj Varshini , Kumar Priyanka , Brinson Jessica , Wang Jiangxia , Samuel Laura , Scott Adrienne , Swenor Bonnielin
Published: December 2022

We examined racial disparities in household food insecurity among low-income Americans with and without vision impairment in the National Health Interview Survey, years 2011-2018. Among 257,620 U.S. adults below a threshold of 150% poverty, 15% of White, 16% of Black, and 8% of Asian Americans, and 18% of American Indians and Inuits reported vision impairment. In analyses adjusted for sociodemographic variables, vision impairment was associated with >100% greater odds (95% CI=2.01-2.31) of 30-day food insecurity, as compared to no vision impairment. Further, odds of household food insecurity were higher among Black Americans (OR=1.37, 95% CI=1.29-1.47), and American Indians and Inuits (OR=1.38, 95% CI=1.15-1.66) than White Americans, while Asians had lower odds (OR=0.45, 95 %CI=0.36-0.57). These findings highlight that low-income adults with vision impairment and racial minorities experience food disparities and dietary inadequacy, an area of disadvantage that can influence overall health, in a nationally representative sample in the U.S.
DEPRESCRIBING IN ASSISTED LIVING
Authors: Zarowitz Barbara , Brandt Nicole
Published: December 2022

Deprescribing is defined as the thoughtful process of “tapering, stopping, discontinuing, or withdrawing drugs, with the goal of managing polypharmacy and improving outcomes.” There are multiple clinical issues that deprescribing can address such as: antibiotic resistance caused by inappropriate and excessive use, the ongoing opioid epidemic, as well as over treatment particularly at the end of life. Networks have been established to address deprescribing across settings including assisted living nationally and internationally. Fourteen key informants from these networks were interviewed including different disciplines. From the interviews, six major themes across two domains were identified. The two domains included regional resources and knowledge gaps and the six themes included: (a) network structure, (b) public perception, (c) policy implications, (d) implementation, (e) challenges, and (f) recommendations. Overall, the importance of collaboration among interprofessional team members will be critical to the success of deprescribing as this clinical issue moves ahead.
Vector unmixing of multicomponent palaeomagnetic data
Authors: Tonti-Filippini Justin A D , Gilder Stuart A
Published: December 2022

Palaeomagnetic investigations often encounter multiple magnetization components, where secondary processes have obscured, partially overprinted or completely replaced the original (primary) remanent magnetization. Identification and separation of primary and secondary magnetizations are generally carried out with principal component analysis of stepwise demagnetization data. However, rocks may contain multiple generations of magnetic minerals with overlapping unblocking ranges that complicate the discrimination of components when applying best-fitting line procedures. Developing a method to differentiate and quantify contributions of overlapping magnetic components using directional data is therefore highly desirable. This paper presents a method to unmix stepwise demagnetization data using an inverse modelling approach. We show that the method is capable of accurately resolving two or three magnetic components with overlapping or superimposed unblocking spectra as well as quantifying absolute component contributions. The method depends on accurate identification and selection of end-member components prior to analysis; in doing so, the method can help palaeomagnetists understand how magnetization components combine to explain their data. We show that the dilution of one component by more than ca. 25 per cent from another component can result in linear demagnetization curves that decay to the origin on orthogonal plots, but whose best-fitting direction can significantly deviate from both end-members. The efficacy of the method is demonstrated through examples of demagnetization data from hematite and/or magnetite-bearing sandstones from China. This method can be broadly applied to all multicomponent magnetization problems in palaeomagnetism.
Human TrkAR649W mutation impairs nociception, sweating and cognitive abilities: a mouse model of HSAN IV
Authors: Pacifico Paola , Testa Giovanna , Amodeo Rosy , Mainardi Marco , Tiberi Alexia , Convertino Domenica , Arevalo Juan Carlos , Marchetti Laura , Costa Mario , Cattaneo Antonino , Capsoni Simona
Published: December 2022

A functional nerve growth factor NGF–Tropomyosin Receptor kinase A (TrkA) system is an essential requisite for the generation and maintenance of long-lasting thermal and mechanical hyperalgesia in adult mammals. Indeed, mutations in the gene encoding for TrkA are responsible for a rare condition, named Hereditary Sensory and Autonomic Neuropathy type IV (HSAN IV), characterized by the loss of response to noxious stimuli, anhidrosis and cognitive impairment. However, to date, there is no available mouse model to properly understand how the NGF–TrkA system can lead to pathological phenotypes that are distinctive of HSAN IV. Here, we report the generation of a knock-in mouse line carrying the HSAN IV TrkAR649W mutation. First, by in vitro biochemical and biophysical analyses, we show that the pathological R649W mutation leads to kinase-inactive TrkA also affecting its membrane dynamics and trafficking. In agreement with the HSAN IV human phenotype, TrkAR649W/m mice display a lower response to thermal and chemical noxious stimuli, correlating with reduced skin innervation, in addition to decreased sweating in comparison to TrkAh/m controls. Moreover, the R649W mutation decreases anxiety-like behavior and compromises cognitive abilities, by impairing spatial-working and social memory. Our results further uncover unexplored roles of TrkA in thermoregulation and sociability. In addition to accurately recapitulating the clinical manifestations of HSAN IV patients, our findings contribute to clarifying the involvement of the NGF–TrkA system in pain sensation.
Structural change, productive development, and capital flows: does financial “bonanza” cause premature deindustrialization?
Authors: Botta Alberto , Yajima Giuliano Toshiro , Porcile Gabriel
Published: December 2022

Recent contributions to the literature on industrialization and development have confirmed that manufacturing continues to play a key role as a driver of economic development. As a corollary, these contributions highlight the importance of premature industrialization as a barrier to economic development and as one of the main sources of the middle-income trap. In this paper, we analyze the factors that may have hindered industrial development in the past four decades. In particular, we focus on the role of (non-Foreign Direct Investment) net capital inflows as a potential source of premature deindustrialization. We consider a sample of 36 developed and developing countries from 1980 to 2017, with major emphasis on the case of emerging and developing economies (EDEs) in the context of increasing financial integration. We show that periods of abundant capital inflows may have caused a significant contraction of manufacturing share to employment and GDP, as well as the decrease of the economic complexity index. We also show that the phenomena of “perverse” structural changes are significantly more relevant in EDE countries than in advanced ones and that they may similarly occur across EDE countries, regardless of structural differences in the way manufacturing contributed to their development. Based on such evidence, we conclude with some policy suggestions highlighting capital controls and external macroprudential measures taming international capital mobility as useful policy tools for promoting long-run productive development on top of strengthening (short-term) financial and macroeconomic stability.
A degenerating convection–diffusion system modelling froth flotation with drainage
Authors: Bürger Raimund , Diehl Stefan , Martí M Carmen , Vásquez Yolanda
Published: December 2022

Froth flotation is a common unit operation used in mineral processing. It serves to separate valuable mineral particles from worthless gangue particles in finely ground ores. The valuable mineral particles are hydrophobic and attach to bubbles of air injected into the pulp. This creates bubble-particle aggregates that rise to the top of the flotation column where they accumulate to a froth or foam layer that is removed through a launder for further processing. At the same time, the hydrophilic gangue particles settle and are removed continuously. The drainage of liquid due to capillarity is essential for the formation of a stable froth layer. This effect is included into a previously formulated hyperbolic system of partial differential equations that models the volume fractions of floating aggregates and settling hydrophilic solids [R. Bürger, S. Diehl and M.C. Martí, IMA. J. Appl. Math.  84 (2019) 930–973]. The construction of desired steady-state solutions with a froth layer is detailed and feasibility conditions on the feed volume fractions and the volumetric flows of feed, underflow and wash water are visualized in so-called operating charts. A monotone numerical scheme is derived and employed to simulate the dynamic behaviour of a flotation column. It is also proven that, under a suitable Courant-Friedrichs-Lewy condition, the approximate volume fractions are bounded between zero and one when the initial data are.
Decentering Hegemony and “Open” Orders: Fifteenth-Century Melaka in a World of Orders
Authors: Pardesi Manjeet S
Published: December 2022

I explain the differences between centered and decentered systems (world orders) by adopting a relational approach to international relations. I argue that centric systems have three characteristics: most meaningful social relations are oriented toward one center; this center establishes the practices governing social relations; and the center recognizes no peers. Conversely, if these attributes are not the chief hallmarks of a social system, then it is decentered. More specifically, I analyze fifteenth-century Melaka to show that simultaneous participation in multiple world orders is a distinct response to hegemony that decenters it. Melaka recognized Ming hegemony but decentered it by also participating in the Persian cosmopolis, and by emerging as a (mini-)center in maritime Southeast Asia. My analysis has two main implications. First, I make the case for “open” orders—multiple, (partially) overlapping orders as viable and long-lasting systems—in contrast to the realist and liberal logics of orders of exclusion/inclusion. Second, this has implications for contemporary Southeast Asian “choices” in the context of the US–China rivalry as these states are likely to “openly” participate in the American and Chinese world orders simultaneously instead of choosing between them. My relational perspective rooted in global history argues against treating historical East Asia as “closed” for such a view is prone to both Sinocentrism and Eurocentrism. Instead, I emphasize the pivotal role played by Melaka—that pursued the strategy of attraction to manage its unequal world—and make the case for “open” orders without flattening power hierarchies or cultural differences.
How cosmic rays mediate the evolution of the interstellar medium
Authors: Simpson Christine M , Pakmor Rüdiger , Pfrommer Christoph , Glover Simon C O , Smith Rowan
Published: December 2022

We explore the impact of diffusive cosmic rays (CRs) on the evolution of the interstellar medium (ISM) under varying assumptions of supernova explosion environment. In practice, we systematically vary the relative fractions of supernovae (SN) occurring in star-forming high-density gas and those occurring in random locations decoupled from star-forming gas to account for SN from run-away stars or explosions in regions that have been cleared by prior SN, stellar winds, or radiation. We find that in the simple system of a periodic stratified gas layer the ISM structure will evolve to one of two solutions: a ‘peak driving’ state where warm gas is volume filling or a ‘thermal runaway’ state where hot gas is volume filling. CR pressure and transport are important factors that strongly influence the solution state the ISM reaches and have the ability to flip the ISM between solutions. Observable signatures such as gamma-ray emission and H i gas are explored. We find that gamma-ray luminosity from pion decay is largely consistent with observations for a range of model parameters. The thickness of the H i gas layer may be too compact, however, this may be due to a large cold neutral fraction of mid-plane gas. The volume fraction of hot gas evolves to stable states in both solutions, but neither settles to a Milky Way-like configuration, suggesting that additional physics omitted here (e.g. a cosmological circumgalactic medium, radiation transport, or spectrally resolved and spatially varying CR transport) may be required.
SAMI-H i: the connection between global asymmetry in the ionized and neutral atomic hydrogen gas in galaxies
Authors: Watts Adam B , Cortese Luca , Catinella Barbara , Power Chris , Fraser-McKelvie Amelia , Bryant Julia J , Croom Scott M , van de Sande Jesse , Bland-Hawthorn Joss , Groves Brent
Published: December 2022

Observations of the neutral atomic hydrogen (${\rm H\, {\small I}}$) gas in galaxies are predominantly spatially unresolved, in the form of a global ${\rm H\, {\small I}}$ spectral line. There has been substantial work on quantifying asymmetry in global ${\rm H\, {\small I}}$ spectra (‘global ${\rm H\, {\small I}}$ asymmetry’), but due to being spatially unresolved, it remains unknown what physical regions of galaxies the asymmetry traces, and whether the other gas phases are affected. Using optical integral field spectrograph (IFS) observations from the Sydney AAO Multi-object IFS (SAMI) survey for which global ${\rm H\, {\small I}}$ spectra are also available (SAMI-${\rm H\, {\small I}}$), we study the connection between asymmetry in galaxies’ ionized and neutral gas reservoirs to test if and how they can help us better understand the origin of global ${\rm H\, {\small I}}$ asymmetry. We reconstruct the global Hα spectral line from the IFS observations and find that while some global Hα asymmetries can arise from disturbed ionized gas kinematics, the majority of asymmetric cases are driven by the distribution of Hα-emitting gas. When compared to the ${\rm H\, {\small I}}$, we find no evidence for a relationship between the global Hα and ${\rm H\, {\small I}}$ asymmetry. Further, a visual inspection reveals that cases where galaxies have qualitatively similar Hα and ${\rm H\, {\small I}}$ spectral profiles can be spurious, with the similarity originating from an irregular 2D Hα flux distribution. Our results highlight that comparisons between global Hα and ${\rm H\, {\small I}}$ asymmetry are not straightforward, and that many global ${\rm H\, {\small I}}$ asymmetries trace disturbances that do not significantly impact the central regions of galaxies.
A polarimetric study of 9 PG quasars with the VLA
Authors: Baghel Janhavi , Kharb P , S. Silpa , Ho Luis C , Harrison C M
Published: December 2022

We present polarization images of 9 radio-loud (RL) quasars from the VLA B-array at 6 GHz. These quasars belong to the Palomar-Green (PG) ‘blazar’ sample comprising 16 RL quasars and 8 BL Lac objects. Extensive polarization is detected in the cores, jets and lobes of all the quasars, with cores primarily displaying magnetic (B-) fields transverse to, and jets displaying fields aligned with the jet direction. Hotspots display either transverse B-fields signifying B-field compression at terminal shocks or more complex structures. The fractional polarization in the cores ranges from 1-10 per cent and jets/lobes from 10-40 per cent. Several of the quasars show distorted or hybrid FRI/FRII radio morphologies with indications of restarted AGN activity. We attribute this to the optical/UV selection criteria of the PG sample that remains unbiased at radio frequencies. The in-band spectral indices of the radio cores are relatively flat while they are steep in the hotspots. This is consistent with the polarization structures where the hotspots appear to be locations of jet bends or bow-shocks. We present global properties for the entire PG ‘blazar’ sample. We find that jet powers correlate with accretion rates for the quasars; higher accretion rates result in more powerful radio jets. A correlation between the radio core fractional polarization and the 150 MHz total radio luminosity for the 9 quasars studied here may imply that more organized B-fields at the jet bases lead to higher core fractional polarization and to more radio powerful radio jets.
First overtone Cepheids of the OGLE Magellanic Cloud Collection – beyond radial modes
Authors: Smolec R , Ziółkowska O , Ochalik M , Śniegowska M
Published: December 2022

We have analysed Optical Gravitational Lensing Experiment photometry for first overtone classical Cepheids in the Large and Small Magellanic Clouds in search for additional periodicities beyond radial modes. We have used standard consecutive pre-whitening technique in some cases followed by time-dependent pre-whitening. We report new candidates for double-mode radial pulsations. However, majority of signals we have detected cannot be interpreted in terms of radial modes. We report 516 double-periodic stars with period ratios, Px/P1O, in the range 0.60–0.65. We study the properties of this class and implications for model explaining these periodicities. We also report 28 stars in which additional variability is of longer period, below radial fundamental mode, with median period ratio, P1O/Px, of 0.684. This class is an analogue of a class known in RR Lyrae stars. Hundreds of other signals were detected that cannot be attributed to radial modes or the above-mentioned classes. Statistical properties of these signals are analysed. We suggest that majority of these signals correspond to non-radial modes. In particular, a significant fraction can be attributed to non-radial modes of moderate degrees, tightly connected to a class with period ratios in between 0.60 and 0.65. In tens of stars, close to radial mode frequency, relatively large-amplitude and coherent signals are observed, which may represent yet another class. In 27 stars periodic modulation of pulsation was detected. Differences in additional frequency content between the two Clouds are discussed.
Probing quasar lifetimes with proximate 21-centimetre absorption in the diffuse intergalactic medium at redshifts z ≥ 6
Authors: Šoltinský Tomáš , Bolton James S , Molaro Margherita , Hatch Nina , Haehnelt Martin G , Keating Laura C , Kulkarni Girish , Puchwein Ewald
Published: December 2022

Enhanced ionizing radiation in close proximity to redshift z ≳ 6 quasars creates short windows of intergalactic Ly α transmission blueward of the quasar Ly α emission lines. The majority of these Ly α near-zones are consistent with quasars that have optically/UV bright lifetimes of $t_{\rm Q}\sim 10^{5}\!-\!10^{7}\rm \, yr$. However, lifetimes as short as $t_{\rm Q}\lesssim 10^{4}\rm \, yr$ appear to be required by the smallest Ly α near-zones. These short lifetimes present an apparent challenge for the growth of $\sim 10^{9}\rm \, M_{\odot }$ black holes at z ≳ 6. Accretion over longer time-scales is only possible if black holes grow primarily in an obscured phase, or if the quasars are variable on time-scales comparable to the equilibriation time for ionized hydrogen. Distinguishing between very young quasars and older quasars that have experienced episodic accretion with Ly α absorption alone is challenging, however. We therefore predict the signature of proximate 21-cm absorption around z ≳ 6 radio-loud quasars. For modest pre-heating of intergalactic hydrogen by the X-ray background, where the spin temperature $T_{\rm S} \lesssim 10^{2}\rm \, K$ prior to any quasar heating, we find proximate 21-cm absorption should be observable in the spectra of radio-loud quasars. The extent of the proximate 21-cm absorption is sensitive to the integrated lifetime of the quasar. Evidence for proximate 21-cm absorption from the diffuse intergalactic medium within $2\!-\!3\rm \, pMpc$ of a (radio-loud) quasar would be consistent with a short quasar lifetime, $t_{\rm Q}\lesssim 10^{5}\rm \, yr$, and would provide a complementary constraint on models for high-redshift black hole growth.
Concerning colour: The effect of environment on type Ia supernova colour in the dark energy survey
Authors: Kelsey L , Sullivan M , Wiseman P , Armstrong P , Chen R , Brout D , Davis T M , Dixon M , Frohmaier C , Galbany L , Graur O , Kessler R , Lidman C , Möller A , Popovic B , Rose B , Scolnic D , Smith M , Vincenzi M , Abbott T M C , Aguena M , Allam S , Alves O , Annis J , Bacon D , Bertin E , Bocquet S , Brooks D , Burke D L , Carnero Rosell A , Carrasco Kind M , Carretero J , Costanzi M , da Costa L N , Pereira M E S , Desai S , Diehl H T , Everett S , Ferrero I , Frieman J , García-Bellido J , Gruen D , Gruendl R A , Gschwend J , Gutierrez G , Hinton S R , Hollowood D L , Honscheid K , James D J , Kuehn K , Kuropatkin N , Lewis G F , Mena-Fernández J , Miquel R , Palmese A , Paz-Chinchón F , Pieres A , Plazas Malagón A A , Raveri M , Rodriguez-Monroy M , Romer A K , Sanchez E , Scarpine V , Schubnell M , Sevilla-Noarbe I , Suchyta E , Swanson M E C , Tarle G , Tucker D L , Weaverdyck N ,   DES Collaboration
Published: December 2022

Recent analyses have found intriguing correlations between the colour (c) of type Ia supernovae (SNe Ia) and the size of their ‘mass-step’, the relationship between SN Ia host galaxy stellar mass (Mstellar) and SN Ia Hubble residual, and suggest that the cause of this relationship is dust. Using 675 photometrically classified SNe Ia from the Dark Energy Survey 5-yr sample, we study the differences in Hubble residual for a variety of global host galaxy and local environmental properties for SN Ia subsamples split by their colour. We find a 3σ difference in the mass-step when comparing blue (c < 0) and red (c > 0) SNe. We observe the lowest r.m.s. scatter (∼0.14 mag) in the Hubble residual for blue SNe in low mass/blue environments, suggesting that this is the most homogeneous sample for cosmological analyses. By fitting for c-dependent relationships between Hubble residuals and Mstellar, approximating existing dust models, we remove the mass-step from the data and find tentative ∼2σ residual steps in rest-frame galaxy U − R colour. This indicates that dust modelling based on Mstellar may not fully explain the remaining dispersion in SN Ia luminosity. Instead, accounting for a c-dependent relationship between Hubble residuals and global U − R, results in ≤1σ residual steps in Mstellar and local U − R, suggesting that U − R provides different information about the environment of SNe Ia compared to Mstellar, and motivating the inclusion of galaxy U − R colour in SN Ia distance bias correction.
Jets from SANE super-Eddington accretion discs: morphology, spectra, and their potential as targets for ngEHT
Authors: Curd Brandon , Emami Razieh , Anantua Richard , Palumbo Daniel , Doeleman Sheperd , Narayan Ramesh
Published: December 2022

We present general relativistic radiation magnetohydrodynamics (GRRMHD) simulations of super-Eddington accretion flows around supermassive black holes (SMBHs), which may apply to tidal disruption events (TDEs). We perform long duration ($t\ge 81,200\, GM/c^3$) simulations that achieve mass accretion rates ≳11 times the Eddington rate and produce thermal synchrotron spectra and images of their jets. Gas flowing beyond the funnel wall expands conically and drives a strong shock at the jet head while variable mass ejection and recollimation, along the jet axis, results in internal shocks and dissipation. Assuming the ion temperature (Ti) and electron temperature (Te) in the plasma are identical, the radio/submillimetre spectra peak at >100 GHz and the luminosity increases with BH spin, exceeding $\sim 10^{41} \, \rm {erg\, s^{-1}}$ in the brightest models. The emission is extremely sensitive to Ti/Te as some models show an order-of-magnitude decrease in the peak frequency and up to four orders-of-magnitude decline in their radio/submillimetre luminosity as Ti/Te approaches 20. Assuming a maximum VLBI baseline distance of 10 Gλ, 230 GHz images of Ti/Te = 1 models shows that the jet head may be bright enough for its motion to be captured with the EHT (ngEHT) at D ≲ 110 (180) Mpc at the 5σ significance level. Resolving emission from internal shocks requires D ≲ 45 Mpc for both the EHT or ngEHT.
Three-dimensional simulations of advective, sub-Keplerian accretion flow on to non-rotating black holes
Authors: Garain Sudip K , Kim Jinho
Published: December 2022

We study the time evolution of sub-Keplerian transonic accretion flow on to a non-rotating black hole using a three-dimensional, inviscid hydrodynamics simulation code. Prior two-dimensional simulations show that centrifugal barrier in the accreting matter may temporarily halt the nearly free-falling matter and produce a stable, geometrically thick disc that may contain turbulent eddies. Our goal in this work is to investigate whether the disc develops any instability because of this turbulence when we dynamically activate all three dimensions. We find that the disc remains stable and axisymmetric even close to the central black hole. However, if we explicitly apply non-axisymmetric azimuthal perturbation, the axisymmetric structure of the disc is destroyed and instability is developed.
CoLoC-seq probes the global topology of organelle transcriptomes
Authors: Jeandard Damien , Smirnova Anna , Fasemore Akinyemi Mandela , Coudray Léna , Entelis Nina , Förstner Konrad U , Tarassov Ivan , Smirnov Alexandre
Published: December 2022

Proper RNA localisation is essential for physiological gene expression. Various kinds of genome-wide approaches permit to comprehensively profile subcellular transcriptomes. Among them, cell fractionation methods, that couple RNase treatment of isolated organelles to the sequencing of protected transcripts, remain most widely used, mainly because they do not require genetic modification of the studied system and can be easily implemented in any cells or tissues, including in non-model species. However, they suffer from numerous false-positives since incompletely digested contaminant RNAs can still be captured and erroneously identified as resident transcripts. Here we introduce Controlled Level of Contamination coupled to deep sequencing (CoLoC-seq) as a new subcellular transcriptomics approach that efficiently bypasses this caveat. CoLoC-seq leverages classical enzymatic kinetics and tracks the depletion dynamics of transcripts in a gradient of an exogenously added RNase, with or without organellar membranes. By means of straightforward mathematical modelling, CoLoC-seq infers the localisation topology of RNAs and robustly distinguishes between genuinely resident, luminal transcripts and merely abundant surface-attached contaminants. Our generic approach performed well on human mitochondria and is in principle applicable to other membrane-bounded organelles, including plastids, compartments of the vacuolar system, extracellular vesicles, and viral particles.
The histone acetyltransferase KAT6A is recruited to unmethylated CpG islands via a DNA binding winged helix domain
Authors: Weber Lisa Marie , Jia Yulin , Stielow Bastian , Gisselbrecht Stephen S , Cao Yinghua , Ren Yanpeng , Rohner Iris , King Jessica , Rothman Elisabeth , Fischer Sabrina , Simon Clara , Forné Ignasi , Nist Andrea , Stiewe Thorsten , Bulyk Martha L , Wang Zhanxin , Liefke Robert
Published: December 2022

The lysine acetyltransferase KAT6A (MOZ, MYST3) belongs to the MYST family of chromatin regulators, facilitating histone acetylation. Dysregulation of KAT6A has been implicated in developmental syndromes and the onset of acute myeloid leukemia (AML). Previous work suggests that KAT6A is recruited to its genomic targets by a combinatorial function of histone binding PHD fingers, transcription factors and chromatin binding interaction partners. Here, we demonstrate that a winged helix (WH) domain at the very N-terminus of KAT6A specifically interacts with unmethylated CpG motifs. This DNA binding function leads to the association of KAT6A with unmethylated CpG islands (CGIs) genome-wide. Mutation of the essential amino acids for DNA binding completely abrogates the enrichment of KAT6A at CGIs. In contrast, deletion of a second WH domain or the histone tail binding PHD fingers only subtly influences the binding of KAT6A to CGIs. Overexpression of a KAT6A WH1 mutant has a dominant negative effect on H3K9 histone acetylation, which is comparable to the effects upon overexpression of a KAT6A HAT domain mutant. Taken together, our work revealed a previously unrecognized chromatin recruitment mechanism of KAT6A, offering a new perspective on the role of KAT6A in gene regulation and human diseases.
Molecular interactions underlying the phase separation of HP1α: role of phosphorylation, ligand and nucleic acid binding
Authors: Her Cheenou , Phan Tien M , Jovic Nina , Kapoor Utkarsh , Ackermann Bryce E , Rizuan Azamat , Kim Young C , Mittal Jeetain , Debelouchina Galia T
Published: December 2022

Heterochromatin protein 1α (HP1α) is a crucial element of chromatin organization. It has been proposed that HP1α functions through liquid-liquid phase separation (LLPS), which allows it to compact chromatin into transcriptionally repressed heterochromatin regions. In vitro, HP1α can undergo phase separation upon phosphorylation of its N-terminus extension (NTE) and/or through interactions with DNA and chromatin. Here, we combine computational and experimental approaches to elucidate the molecular interactions that drive these processes. In phosphorylation-driven LLPS, HP1α can exchange intradimer hinge-NTE interactions with interdimer contacts, which also leads to a structural change from a compacted to an extended HP1α dimer conformation. This process can be enhanced by the presence of positively charged HP1α peptide ligands and disrupted by the addition of negatively charged or neutral peptides. In DNA-driven LLPS, both positively and negatively charged peptide ligands can perturb phase separation. Our findings demonstrate the importance of electrostatic interactions in HP1α LLPS where binding partners can modulate the overall charge of the droplets and screen or enhance hinge region interactions through specific and non-specific effects. Our study illuminates the complex molecular framework that can fine-tune the properties of HP1α and that can contribute to heterochromatin regulation and function.
Sleep duration and cognitive function among older adults with chronic kidney disease: results from the National Health and Nutrition Examination Survey (2011–2014)
Authors: Warsame Fatima , Chu Nadia M , Hong Jingyao , Mathur Aarti , Crews Deidra C , Bayliss George , Segev Dorry L , McAdams-DeMarco Mara A
Published: December 2022

Background

Short and long sleep durations are associated with cognitive dysfunction. Given the increased prevalence of sleep abnormalities in the chronic kidney disease (CKD) population, we tested whether the association between sleep duration and cognitive function differed between older adults with and without CKD.

Methods

This was a study of 3215 older adults (age ≥60 years) enrolled in the National Health and Nutrition Examination Survey (2011–14) evaluating sleep duration, cognitive function (immediate recall, delayed recall, verbal fluency, executive function and processing speed and global cognition) and kidney function. We quantified the association between sleep duration and cognitive function using linear regression and tested whether the associations differed among those with CKD and without using a Wald test for interaction.

Results

Among 3215 participants, 13.3% reported 2–5 hours of sleep/day, 75.2% reported 6–8 hours, and 11.5% reported ≥9 hours. Persons with CKD were more likely to sleep ≥9 hours [odds ratio 1.73 (95% confidence interval 1.22–2.46)]. Among participants with CKD, those with a sleep duration ≥9 hours demonstrated worse global cognitive function (P for interaction = .01), immediate recall (P for interaction = .01) and verbal fluency (P for interaction = .004) than those with a sleep duration of 6–8 h; no differences were observed for participants with CKD who slept 2–5 hours. Among participants without CKD, sleep was not associated with any measures of cognitive function.

Conclusions

Longer sleep duration is associated with worse cognitive function only among persons with CKD, and global cognition, delayed recall and verbal fluency are particularly affected. Studies should identify interventions to improve sleep patterns and quality in this population.
Neural basis of speech and language impairments in development
Authors: Asaridou Salomi S. , Watkins Kate E.
Published: December 2022

Developmental Language Disorder (DLD) is characterized by difficulties in acquiring one’s native language for no apparent reason. While DLD is a common developmental disorder, its neural basis is poorly understood. In this chapter, the authors will provide an overview of neuroimaging studies that investigated brain function and structure of children with DLD using magnetic resonance imaging (MRI). Under-activation in the left fronto-temporal cortex during language processing, atypical grey matter structure in the basal ganglia, and microstructural differences in dorsal fronto-temporal white matter connections are among the more consistent literature findings, although the overall picture that emerges is far from complete. The authors conclude that, moving forward, the field needs a paradigm shift from studies attempting to find a one-to-one brain region-to-behavior mapping to large-scale studies and approaches that take into consideration the brain as a network as well as the dimensionality and complexity within the disorder.
Population-based prevalence and incidence estimates of mixed connective tissue disease from the Manhattan Lupus Surveillance Program
Authors: Hasan Ghadeer , Ferucci Elizabeth D , Buyon Jill P , Belmont H Michael , Salmon Jane E , Askanase Anca , Bathon Joan M , Geraldino-Pardilla Laura , Ali Yousaf , Ginzler Ellen M , Putterman Chaim , Gordon Caroline , Helmick Charles G , Parton Hilary , Izmirly Peter M
Published: December 2022

Objective

Epidemiological data for MCTD are limited. Leveraging data from the Manhattan Lupus Surveillance Program (MLSP), a racially/ethnically diverse population-based registry of cases with SLE and related diseases including MCTD, we provide estimates of the prevalence and incidence of MCTD.

Methods

MLSP cases were identified from rheumatologists, hospitals and population databases using a variety of International Classification of Diseases, Ninth Revision codes. MCTD was defined as one of the following: fulfilment of our modified Alarcon-Segovia and Kahn criteria, which required a positive RNP antibody and the presence of synovitis, myositis and RP; a diagnosis of MCTD and no other diagnosis of another CTD; and a diagnosis of MCTD regardless of another CTD diagnosis.

Results

Overall, 258 (7.7%) cases met a definition of MCTD. Using our modified Alarcon-Segovia and Kahn criteria for MCTD, the age-adjusted prevalence was 1.28 (95% CI 0.72, 2.09) per 100 000. Using our definition of a diagnosis of MCTD and no other diagnosis of another CTD yielded an age-adjusted prevalence and incidence of MCTD of 2.98 (95% CI 2.10, 4.11) per 100 000 and 0.39 (95% CI 0.22, 0.64) per 100 000, respectively. The age-adjusted prevalence and incidence were highest using a diagnosis of MCTD regardless of other CTD diagnoses and were 16.22 (95% CI 14.00, 18.43) per 100 000 and 1.90 (95% CI 1.49, 2.39) per 100 000, respectively.

Conclusions

The MLSP provided estimates for the prevalence and incidence of MCTD in a diverse population. The variation in estimates using different case definitions is reflective of the challenge of defining MCTD in epidemiologic studies.
Phylogenomics of the Ancient and Species-Depauperate Gars Tracks 150 Million Years of Continental Fragmentation in the Northern Hemisphere
Authors: Doran Brownstein Chase , Yang Liandong , Friedman Matt , Near Thomas J
Published: December 2022

Reconstructing deep-time biogeographic histories is limited by the comparatively recent diversification of most extant lineages. Ray-finned fishes, which include nearly half of all living vertebrates, are no exception. Although most lineages of ray-finned fishes radiated around the Cretaceous-Paleogene boundary, a handful of ancient, species-poor clades still persist. These lineages can illuminate very old biogeographic trends, but their low species richness can also limit the reconstruction of these patterns. The seven extant species of gars distributed in freshwater habitats in North America and Cuba are an old clade with a fossil record spanning over 150 million years of Earth history. Using a genomic data set of DNA sequences of 1105 exons for the seven living species and an updated morphological matrix of all extant and extinct taxa, we infer the phylogenetic relationships of gars and test how divergence times and biogeographic reconstructions are influenced by sequential and joint estimation and the effect on these inferences when using different taxon sets based on fossil completeness. Our analyses consistently show that the two extant gar genera Atractosteus and Lepisosteus diverged approximately 105 million years ago and many of the inferred divergences in the gar time-calibrated phylogeny closely track major Mesozoic tectonic events, including the separation of the Americas, the expansion of the early Atlantic, and the Cretaceous reorganization of North American river systems. The crown clades Atractosteus and Lepisosteus originated in the Cenozoic of eastern North America, implying that this region has served as both the origin of extant gar diversity and the refugium of this iconic ancient lineage. These results exemplify how combining phylogenomics with the fossil record provides congruence around the evolutionary history of ancient clades like gars and can reveal long-lost biogeographic patterns. [Lepisosteidae; fossilized birth death; biogeography; phylogenomics; paleontology.]
The Complete Genome Resource of Xanthomonas oryzae pv. oryzae CIX2779 Includes the First Sequence of a Plasmid for an African Representative of This Rice Pathogen
Authors: Sciallano Coline , Auguy Florence , Boulard Gabriel , Szurek Boris , Cunnac Sébastien
Published: December 2022

The bacterial plant pathogen Xanthomonas oryzae pv. oryzae is responsible for the foliar rice bacterial blight disease. Genetically contrasted, continent-specific, sublineages of this species can cause important damages to rice production both in Asia and Africa. We report on the genome of the CIX2779 strain of this pathogen, previously named NAI1 and originating from Niger. Oxford Nanopore long reads assembly and Illumina short reads polishing produced a genome sequence composed of a 4,725,792-bp circular chromosome and a 39,798-bp-long circular plasmid designated pCIX2779_1. The chromosome structure and base-level sequence are highly related to reference strains of African X. oryzae pv. oryzae and encode identical transcription activator-like effectors for virulence. Importantly, our in silico analysis strongly indicates that pCIX2779_1 is a genuine conjugative plasmid, the first indigenous one sequenced from an African strain of the X. oryzae species.

[Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .
Croup
Authors: Hawk Heather
Published: December 2022

Croup, a respiratory condition marked by stridor, hoarse voice, and difficulty breathing, is one of the leading reasons for clinic and ED visits by young children.
Trauma-Informed Care Curricula for the Health Professions: A Scoping Review of Best Practices for Design, Implementation, and Evaluation
Authors: Burns Courtney Julia , Borah Luca , Terrell Stephanie M. , James LaTeesa N. , Erkkinen Elizabeth , Owens Lauren
Published: December 2022

Purpose

Trauma-exposed persons often experience difficulties accessing medical care, remaining engaged in treatment plans, and feeling psychologically safe when receiving care. Trauma-informed care (TIC) is an established framework for health care professionals, but best practices for TIC education remain unclear. To remedy this, the authors conducted a multidisciplinary scoping literature review to discern best practices for the design, implementation, and evaluation of TIC curricula for health care professionals.

Method

The research team searched Ovid MEDLINE, Cochrane Library, Elsevier’s Scopus, Elsevier’s Embase, Web of Science, and the PTSDpubs database from the database inception date until May 14, 2021. Worldwide English language studies on previously implemented TIC curricula for trainees or professionals in health care were included in this review.

Results

Fifty-five studies met the inclusion criteria, with medicine being the most common discipline represented. The most prevalent learning objectives were cultivating skills in screening for trauma and responding to subsequent disclosures (41 studies [74.5%]), defining trauma (34 studies [61.8%]), and understanding trauma’s impact on health (33 studies [60.0%]). Fifty-one of the studies included curricular evaluations, with the most common survey items being confidence in TIC skills (38 studies [74.5%]), training content knowledge assessment (25 studies [49.0%]), participant demographic characteristics (21 studies [41.2%]), and attitudes regarding the importance of TIC (19 studies [37.3%]).

Conclusions

Future curricula should be rooted in cultural humility and an understanding of the impacts of marginalization and oppression on individual and collective experiences of trauma. Moreover, curricula are needed for clinicians in more diverse specialties and across different cadres of care teams. Additional considerations include mandated reporting, medical record documentation, and vicarious trauma experienced by health care professionals.
Drama Training as a Tool to Teach Medical Trainees Communication Skills: A Scoping Review
Authors: Landry-Wegener Bernard A. , Kaniecki Timothy , Gips Julia , Lebo Rachael , Levine Rachel B.
Published: December 2022

Purpose

Recognizing the similarities between the skills an actor needs and those required of a physician in clinical communication, medical educators have begun to create drama-based interventions to teach communication skills. The purpose of this scoping review was to summarize existing educational interventions that use drama training to teach medical trainees communication skills.

Method

The authors searched PubMed, CINAHL Plus, Embase, ERIC, and Web of Science Core Collection multiple times beginning in March 2020 through March 2022. Articles were included if they (1) described components of an educational intervention, (2) used an active intervention based on drama training, (3) stated a curricular goal of improving learners’ communication skills, and (4) included medical trainee learners. Data extracted included the details of the targeted learners and educational interventions, assessment tools, and outcomes. The quality of each study was assessed.

Results

Thirty articles met the inclusion criteria. Twenty-five (83%) articles included acting and improvisation exercises, 9 (30%) used some or all of the conventions of forum theater, and 3 (10%) used dramatic performance. The interventions included undergraduate, graduate, and continuing medical education learners. Most were elective and involved a member of the theatrical community. Although low overall study quality (average MERSQI score was 8.5) limited the strength of the evidence, of the 8 articles that evaluated learners’ knowledge, skills, and/or behaviors, the majority showed an initial improvement in communication skills post-intervention.

Conclusions

This review is the first, to the authors’ knowledge, to focus on the curricular goal of improving communication skills and to include a broader scope (beyond medical improv) of drama trainings. While the included articles represent a diverse group of interventions, generally they reported an outcome of improved communication skills in their learners. More high-quality studies are needed to determine best practices and the generalizability of drama-based initiatives.