bluuebunny/crossref_metadata_2025 huggingface dataset:
Building of export performance through product innovation, trust, technology capability and partnerships (a study on furniture industry in Jepara Regency of Indonesia)
Authors:   Nahdlatul Ulama Islamic University, Arifin Samsul
Published: August 2021

This study aims to analyze the variables that affect furniture exports’ performance in Jepara, Indonesia, in the year 2020. A quantitative research approach is applied based on data of 307 furniture companies in Jepara, exporters in the international furniture trade market, with the desired error limit of 5%. Data consists of two types, namely primary data and secondary data. The primary data were obtained through a questionnaire that was personally submitted to 174 respondents. Secondary data used as a basis for determining the techniques and steps for collecting research data were obtained from BPS Jepara Regency and Jepara Regency Industry and Trade Office, in the form of Jepara Furniture SME (structural equation models) export volume in 2016-2020 and Jepara furniture exporters data for 2020. The results show that product innovation, trust, technological capabilities and partnerships are variables that affect the performance of furniture exports in the Jepara district.
Analysis of green supply chain management performance with green supply chain operation reference at the batik enterprise
Authors:   Universitas Muhammadiyah Surakarta, Munawir Hafidh , Mabrukah Putri Rona ,   Universitas Muhammadiyah Surakarta, Djunaidi Much ,   Universitas Muhammadiyah Surakarta, Suranto  ,   Universitas Muhammadiyah Surakarta
Published: August 2021

Nowadays, the Hayuningrum Batik Industry keeps trying to optimize the production of batik cloth until the batik cloth can be accepted by consumers, but the Hayuningrum Batik Industry lacks awareness about environmental issues. One effort that can be taken is by measuring the performance of Green Supply Chain Management (GSCM). This study aims to calculate and analyze GSCM performance in the Hayuningrum Batik Industry in the year 2020 and provide proposed improvement to improve GSCM performance in the Hayuningrum Batik Industry located in Laweyan, Surakarta. The method used are the Green Supply Chain Operation Reference (Green SCOR) model approach, weighting with the Analytical Hierachy Process (AHP) method, and normalization using the Snorm de Boer formula. Based on research conducted, 26 Key Performance Indicators (KPI) have been verified. The KPI is weighted using the AHP method. Then, the normalization score has been calculated for each indicator. The results of these measurements show the value of GSCM performance in the Hayuningrum Batik Industry of 57.42, which means that the performance is classified as Average. Therefore, the Hayuningrum Batik Industry need to improve their performance. The proposed improvement is given to each indicator in the Hayuningrum Batik Industry that need to be prioritized for improvement.
Public financial control in Ukraine: state, problems, prospects
Authors:   Vinnytsia National Agrarian University, Kaletnik Hryhorij , Zdyrko Nataliya ,   Vinnytsia National Agrarian University
Published: August 2021

The authors assess the current state of public financial control in Ukraine, identifies its main problematic aspects and suggests ways to solve them. There is a direct interdependence between increasing the level of corruption and weakening the effectiveness of public financial control. Forms and types of public financial control are considered depending on controlling subjects. The authors identify forms of public sector audit according to the ISSAI standards which include financial audit, performance audit and compliance audit. The paper touches upon the necessity of legislative introduction of audit of conformity with the corresponding definition of the purpose, tasks, subjects, objects, methods and procedures. It is proposed to implement the Standard for Auditing the Compliance of Public Resources, which will define general provisions, principles and criteria of compliance audit, as well as the procedure for planning, directions, methods and control points in its implementation, generalization and implementation of results. The main generalized indicators of activity of the Accounting Chamber in the period between 2009 and 2019 are presented and the basic trends in the development of the public financial control are covered. It characterizes the number of inspected objects, prepared reports, the volume of detected violations, the number of relevant response documents and the amount of estimated funding. The structure of identified violations in the main areas includes violations of the budget legislation, inefficient management of funds and violations of the administration of the revenue side of the state budget. The basic criteria for carrying out performance audit are defined and their analytical assessment is provided. The main directions of improvement of the public financial control with the use of key characteristics of the concept of good governance are offered, the fundamentals of which include the rule of law, equality, inclusiveness, efficiency, transparency and accountability. It is recommended to introduce and use a unified report on budget offenses, which will facilitate communication between regulatory authorities and allow for receiving, processing and storeing large amounts of information on the results of auditing.
Behaviour models of audit quality reduction associated with auditor’s work stress
Authors:   Hasanuddin University, Arifuddin Mannan , Darwis Said ,   Hasanuddin University, Sundari Sri ,   Hasanuddin University
Published: August 2021

The current study’s main aim is to examine a behaviour model of reducing audit quality in relation to auditor work stress. The study was performed in 2020 and the cases in the current research were auditors of the Audit Board of the Republic of Indonesia (BPK), auditors of the Development Finance Audit Board (BPKP) and public accounting firm (KAP) auditors in South Sulawesi Province. The sample numbers is determined according to the sample size needed via data investigation employing the Partial Least Square approach, using a random sampling method. The data collected by utilizing a questionnaire and questionnaire data, which can be analyzed as many as 97. The output of this study indicate: (1) the long period pressure cause increase auditor working stress; (2) the long period pressure cause the reduction of audit quality behaviour; (3) the long period conflict cause increase auditors’ working stress; (4) the high role conflict cause increasement the audit quality reduction behaviour; (5) external locus of control cause increasement auditors’ working stress; (6) external locus of control will increase audit quality reduction behaviour, and (7) high work stress cause reduction of audit quality behaviour.
Pemberdayaan Ekonomi Masyarakat Melalui Budidaya Enceng Gondok
Authors: Farika Rina
Published: August 2021

Penelitian ini yang bertujuan untuk suatu pemberdayaan masyarakat melalui budidaya enceng gondok. Salah satu cara untuk mensukseskan pembangunan yang ada di Desa Sumberejo, yaitu dengan cara meningkatkan pendapatan desa. Besar kecilnya pendapatan oleh desa tergantung strategi yang dilakukan oleh desa dalam mengelola. Tingkat perekonomian yang rendah masyarakat yang membuat pemerintah desa tergerak untuk meningkatkan perekonomian masyarakat melalui budidaya enceng gondok. Rumusan masalah dalam penelitian adalah bagaimana proses dari pemberdayaan masyarakat dengan cara pemanfaatn enceng gondok yang dilakukan oleh kelompok masyarakat desa sumberejo. Serta bagaimana faktor pendukung dan penghambat pemberdayaan masyarakat yang dilakukan oleh kelompok masyarakat melalui pemanfaatan enceng gondok. Penelitian ini menggunakan pendekatan kualitatif, dengan subjek penelitian yaitu, pengelola, tenaga kerja, dan masyarakat desa. Pengumpulan data menggunakan metode wawancara, observasi, dan dokumentasi. Hasil dari penelitian ini menunjukan proses pemberdayaan masyarakat melalui pemanfatan enceng gondok yang dilakukan oleh kelompok masyarakat desa dengan beberapa tahap, penyadaran terhadap lingkungan, pengkapasitasan, dan evaluasi. Faktor pendukung yang dilakukan oleh masyarakat adalah banyaknya enceng gondok, pemasaran yang mudah, meningkatkan perekonomian masyarakat. Sedangkan faktor penghambat adalah faktor cuaca, kurangnya sarana dan prasarana yang kurang memadai, serta pembuatan yang sulit sehingga membutuhkan waktu yang lama.
Pesantren for Middle-Class Muslims in Indonesia (Between Religious Commodification and Pious Neoliberalism)
Authors: Hidayah Siti Nur
Published: August 2021

Research about Islamic educational institutions, the market and the rise of the new Muslim middle-class in Indonesian society has mainly focused on schools. Its correlation with pesantren (Islamic boarding schools) as Islamic education providers has not yet been deeply portrayed. This paper aims to identify changes in pesantren management practices in relation to the growth of the Muslim middle-class and questions whether pesantren management practices intended to cater for the middle-class segment of society can be categorized as commodification or as acts of pious neoliberalism. As a preliminary examination, this paper was based on extensive literature and media research, interviews with teachers and parents in pesantren, and non-participant observation. This research highlights three different strategies developed by pesantren to respond to the growing size of the Muslim middle-class in Java, Indonesia: ‘developing’, ‘inserting’ and ‘creating’ new pesantren education programs. Three models are highlighted here in three select pesantren in Java: Firstly, a pesantren established and designed to accommodate middle-class Muslims that employs an approach that is an amalgamation between religious education and international educational standards. Secondly, a well-established traditional pesantren which built new ‘elite’ buildings to respond to demand from middle-class Muslims. And thirdly, a pesantren that targets urban middle-class students of all ages who have limited religious knowledge and which mainly focuses on a tahfidz program (memorizing of the Qur’an) through creating a ‘friendly’ image of learning the Qur’an. These pesantren maintain a deeply religious curriculum similar to traditional pesantren and provide good facilities for students but charge high fees for education, and as such may connotate a commodification practice. Using Mona Atia’s concept of pious neoliberalism, the writer questions whether the fusion of religious practices of any kind, commodification and adjustment to market logic, in this context, might be better understood as pious neoliberalism. In this sense, the commodification practices in the examples offered here should not always bear a pejorative meaning. While admitting that global changes have introduced new challenges to the Muslim community and in relation to Islamic education, it is hoped that this article will encourage further discussion and investigation on the subject of the changing nature of provision and management of Islamic educational institutions, in particular pesantren, in Indonesia.
A view from Slovakia on the study of Russian philosophical narodism (P. L. Lavrov: the discourse of a study and the discourse of a street)
Authors:   University of Presov, Marchevsky Ondrej
Published: August 2021

The paper analyzes Slovakian and Czech studies of the sociological a philosophical views of such Russian narodniks as P.L. Lavrov from a historic point of view and in their rela­tion to some contemporary interpretations. The first part of the paper provides an over­view of Czech and Slovakian works on narodism in Russia. In the second part of the pa­per, the author discusses P.L. Lavrov as an outstanding representative of narodism, whose ideas have not received sufficient attention in the Czech and Slovakian scholarship. The author shows that the study and the street as two places in which Lavrov’s thought devel­oped were two points in the social space within which the former represented theory and the latter represented practice. Lavrov’s discourse of the study is built on objectivity and scientific values, whereas his discourse of the street is characterized by radicalism and in­tolerance to different opinions. This duality in Lavrov’s perspective reflects the duality of his character: on the one hand, he was a scientist and a researcher and, on the other, he was a radical revolutionary.
Another outlook on Russia. Letters from the “Russian Archive”
Authors: Walicki Andrzej ,   Lomonosov Moscow State University
Published: August 2021

The article presents previously unpublished letters written by Andrzej Walicki (15.05.1930–21.08.2020), a worldly renowned Polish historian of Russian thought, to Professor Michael Maslin, the head of the Department of the History of Russian Philoso­phy at Lomonosov Moscow State University. Walicki’s letters (1997–2019) together with books, articles and other materials formed his gift to the abovementioned Department. Walicki himself referred to these materials as “my small Russian archive”. The letters are written in excellent Russian and require no additional revision or stylistic improvement. This publication retains the letters in their full originality including some phrases of Pol­ish origin. These unique epistles reveal Walicki’s individual creative worldview. The let­ters contain new information about the details of Walicki’s biography and his work in Poland, Russia, USA, Great Britain, Japan, Australia. The letters provide a unique per­spective on the “flow of ideas”, which was Walicki’s personal conception of understand­ing and interpretation of the Russian intellectual history from the Enligh­tenment through the Russian religious and philosophical Renaissance of the twentieth century. The letters discuss his interactions with Sergei Gessen, Isaiah Berlin, Leszhek Kolakowski, Czeslaw Milosz, George Kline, James Scanlan, Leonard Shapiro, Martin Malia, Richard Pipes, Nicholas Riasanovsky, James Billington etc. A special attention is paid to the critique of the Western and especially Polish Russophobia based on various superstitions and stereo­types about Russia as well on a lack of knowledge, various kinds of bias and blunders. Of considerable interest are Walitsky’s expert assessments of the ge­neral state of the scien­tific historiography of Russian philosophy, its fundamental diffe­rences from Soviet dog­matic Marxism, of which the Polish scientist was a consistent critic.
Alexius Meinong’s theory of relations
Authors:   Lomonosov Moscow State University, Mironov Dmitry G.
Published: August 2021

The purpose of the article is to analyze the theory of relations developed by A. Meinong and to demonstrate its significance for the formation of Meinong’s theory of objects. The article is divided into three parts. In the first part, the author shows that the interest in a theory of relations was not accidental for the representatives of the school of Bren­tano: one cannot determine the characteristic features of intentional relations without determining the characteristic features of relations as such. The article considers some ele­ments of the theory of relations proposed by Brentano and by some of his closest disci­ples – A. Marty and C. Stumpf. The second part deals with two important topics in Meinong’s theory of relations: classification of relations and their status. It is shown that Meinong recognized the distinctive ontological status of relations and their indepen­dence from the cognitive activity of mind. It is also shown that an attempt to clarify the characteristic features of “ideal” relations made by Meinong within the frame­work of his classification of relations eventually led him to formulate one of the key theses of his the­ory of objects. Finally, the third part discusses the nature of relations. The author analyses two solutions considered by Meinong: the first one, suggested in 1899, presupposed that some other relations connect a given relation with its relata, and the second one, devel­oped in 1902, was a suggestion that each relation by itself connects its relata. It is shown that the second solution required Meinong to expand the ontology and to change the pre­vious epistemology. A possible contemporary interpre­tation of the second solution pro­posed by Meinong is also suggested.
To the question of evidence in Husserl’s phenomenology: the given­ness and the horizon
Authors:   Russian Presidential Academy of National Economy and Public Administration, Belousov Mikhail A.
Published: August 2021

The article deals with the initial context of introduction and subsequent transformation of the concept of evidence in Husserlian phenomenology. It shows that the initial context is composed of the basic differences drawn in the theory of meaning of Logical Investi­gations. These differences include the difference between experience, meaning and object as well as the correlative differences between meaning-intention and meaning-fulfillment, on the one hand, and meaning and fulfilling sense, on the other. The proposed analysis of these distinctions allows the author to explicate the two main interpretations of the notion of evidence: the strict and the lax meaning. The second section reveals the distinction be­tween the horizon and the givenness. This distinction plays a key role in the treatment of evidence in transcendental phenomenology as a mere result of a methodical interpretation of the differences drawn in the theory of meaning and the theory of evidence in Logical Investigations. It is demonstrated that these differences imply key methodical intuitions of Husserlian phenomenology. In conclusion, the naïve origins of the principle of evi­dence in phenomenology are thematized.
The affordances and limitations of collaborative research in the TESOL classroom
Authors: Slaughter Yvette , Choi Julie , Nunan David , Black Hayley , Grimaud Rebecca , Trinh Hân
Published: August 2021

The diversity of learning needs within the TESOL field creates inherent tensions between the need for targeted professional learning for TESOL teachers, the more generalist nature of tertiary TESOL courses, and the varied research interests of teacher educators. This article describes a collaborative research project between university-based teacher educators and TESOL teachers working in an adult education centre. With a range of aims amongst the research participants, this article reports on the ‘fluid’ and ‘messy’ process of collaborative research (Burns & Edwards, 2014, p. 67) as we investigate the use of identity texts (Cummins & Early, 2011) as a mediating tool for professional learning. In acknowledging the practice of teaching as highly situated, the data presented focuses on the individual experience of each teacher, voiced through an action research frame, before we discuss the achievements and challenges which emerged through this collaborative research process. In the findings, we argue for the importance of championing the case for the messy processes of collaborative research within the broader research academy.
Integrated Metabolic and Gene Expression Profiling Reveals New Therapeutic Modalities for Rapidly Proliferating Breast Cancers
Authors: Liao Chengheng , Glodowski Cherise Ryan , Fan Cheng , Liu Juan , Mott Kevin Raynard , Kaushik Akash , Vu Hieu , Locasale Jason , McBrayer Samuel K , DeBerardinis Ralph , Perou Charles , Zhang Qing
Published: August 2021

Metabolic dysregulation, although a prominent feature in breast cancer, remains undercharacterized in patient tumors. By performing untargeted metabolomics analyses on triple-negative breast cancer (TNBC) and Estrogen Receptor (ER) positive patient breast tumors, as well as TNBC patient-derived xenografts (PDXs), we identified two major metabolic groups independent of breast cancer histological subtypes: a “Nucleotide/Carbohydrate-Enriched” group and a “Lipid/Fatty Acid-Enriched” group. Cell lines grown in vivo more faithfully recapitulate the metabolic profiles of patient tumors. Integrated metabolic and gene expression analyses reveal genes that strongly correlate with metabolic dysregulation and predict patient prognosis. As a proof-of-principle, targeting Nucleotide/Carbohydrate-Enriched TNBC cell line or PDX xenografts with a pyrimidine biosynthesis inhibitor, and/or a glutaminase inhibitor, led to therapeutic efficacy. In addition, the pyrimidine biosynthesis inhibitor presents better therapeutic outcomes than chemotherapy agents in multiple murine TNBC models. Our study provides a new stratification of breast tumor samples based on integrated metabolic and gene expression profiling, which guides the selection of newly effective therapeutic strategies targeting rapidly proliferating breast cancer subsets. In addition, we develop a public, interactive data visualization portal (http://brcametab.org) based on the data generated from this study.
Complete genome sequence of Chryseobacterium sp. ZHDP1 with protease activity
Authors: Zhao Huanshuai , Wang Jianxin , Huang Jiamao , Ma Yuncheng , Chen Yunfei , Tao Chenzhi , Wang Jiangqi , Jin Linqian , Wang Dingquan , Qu Wu
Published: August 2021

This study reported a complete genome of Chryseobacterium sp. ZHDP1 isolated from the soils of a seafood market. The ZHDP1 genome with a size of 4,917,748 bp and a GC content of 35.95% possessed 4,478 coding genes, 5 rRNA genes, 26 sRNA genes, and 89 tRNA genes. The 16S rRNA gene sequence of ZHDP1 had a maximum similarity of 99.07% with that of C. gambrini 5-1St1a. The maximum values of average nucleotide identity and DNA-DNA hybridization of ZHDP1 genome were 91.39 and 47.8, respectively, which were lower than the thresholds for a new genome. Different protease genes were annotated in the genome of ZHDP1, and the protease activity was also detected in the fermentation broth of ZHDP1. Furthermore, the activity of protease in the fermentation broth was optimized through temperature, pH, and metal irons, and the results showed that 60°C and pH 7.0 were the optimum conditions and Fe3+ could positively increase the protease activity of ZHDP1. This study provides the first insight into the novel genomic information of Chryseobacterium sp. ZHDP1 and its protein-degrading ability, thereby broadening our knowledge of the industrial potentials in genus Chryseobacterium strains.
Trace Metals and Organochlorine Pesticide Residues in Imported Fishes in Bangladesh and Human Health Risk Implications
Authors: Islam Md. Monirul , Avha Nusrat Jahan , Ahmed Shamim , Akbor Md. Ahedul , Islam Md. Sofiqul , Mostafiz Farhana , Habibullah-Al-Mamun Md.
Published: August 2021

Substantial quantity of fish has been imported to Bangladesh without adequate food safety assessment which can pose a serious health risk to local people. This study analyzed the trace metals and organochlorine pesticides residues and the associated human health risk in 33 imported fishes (9 species) from four countries (India, Myanmar, Oman and United Arab Emirates) collected from three different ports (Benapole, Bhomra, and Chittagong) of Bangladesh with invoice lists from the port authorities. Trace metal concentrations were determined using graphite furnace absorption spectrometry and flame absorption spectrometry. The two organochlorine pesticides (Aldrin and Chlordane) residues were determined by GC-MS and found as below detection level (BDL). The trace metal concentrations (mg/kg-ww) in imported fish samples ranged as: As: 0.008 to 0.558; Pb: 0.004 to 0.070; Cr: 0.010 to 0.109; Cd: 0.00 to 0.083; Ni: 0.011 to 0.059; Co: BDL to 0.067; Mn: BDL to 0.0780; Fe: 1.780 to 10.77; Cu: 0.055 to 0.632; and Zn: 0.898 to 9.245. Concentrations of As and Cd were higher than the food safety guideline. Considering the source country of imported fishes, fish samples from Oman were mostly contaminated by the trace metals. The estimated daily intake (EDI) was higher for As and Cr. However, the target hazard quotient (THQ) for individual metal and total THQ for combined metals were lower than 1, indicating no apparent non-carcinogenic health risk for consumers. But target cancer risk (TR) was higher for As, and Ni and the values exceeded the acceptable range indicating a high carcinogenic risk for the local people. Therefore, extensive monitoring of these toxic chemicals is needed prior to import these fishes to the country. Given the self-sufficiency in fish production this study also argues whether Bangladesh needs to import the fishes at all.
Neurophysiological Effects of Dry Needling in Patients with Neck Pain, A Randomized Clinical Trial
Authors: Martín-Sacristán Luis , Calvo-Lobo Cesar , Pecos-Martín Daniel , Fernández-Carnero Josue , Alonso-Pérez Jose Luis
Published: August 2021

The purpose was to determine the efficacy of deep dry needling (DDN) applied on an active myofascial trigger point (MTrP) versus a latent-MTrP versus a non-MTrP location, on pain reduction and cervical disability, in patients with chronic neck pain. A randomized, double-blind clinical trial design was used. A sample of 65 patients was divided into non-MTrP-DDN, active-MTrP-DDN and latent-MTrP-DDN groups. The visual analog scale (VAS), reproduction of the patient’s pain, number of local twitch responses, pressure pain threshold (PPT) and Neck Disability Index (NDI) were assessed before, during and after the intervention and up to 1 month post-intervention. The active-MTrP-DDN-group reduced pain intensity more than non-MTrP-DDN-group after a week and a month (p<0.01). Active-MTrP-DDN-group showed the greatest improvement in tibialis muscle PPT. An association was found with a higher percentage of subjects in whom their neck pain was reproduced when the active-MTrP (77.3%) and the latent-MTrP (81.8%) were treated. The application of DDN on an active-MTrP in the upper trapezius muscle shows greater improvements in pain intensity after one week and one month post-intervention, as well as lesser improvement in PPT in the tibialis muscle, compared to DDN applied in latent-MTrPs or outside of MTrPs in patients with neck pain
Constrained Control Methods for Lower Extremity Rehabilitation Exoskeleton Robot Considering Unknown Perturbations
Authors: Tian Jin , Yuan Liang , Xiao Wendong , Ran Teng , Zhang Jianbo , He Li
Published: August 2021

In this paper, trajectory tracking control is investigated for lower extremity rehabilitation exoskeleton robot. Unknown perturbations are considered in the system which are inevitable in the reality. The trajectory tracking control is constructively treated as constrained control issue. To obtain the explicit equation of motion and analytical solution of lower extremity rehabilitation exoskeleton robot, Udwadia-Kalaba theory is introduced. Lagrange multipliers and pseudo variables are not needed in Udwadia-Kalaba theory, which is more superior than Lagrange method. On the basic of Udwadia-Kalaba theory, two constrained control methods including trajectory stabilization control and adaptive robust control are proposed. Trajectory stabilization control applies Lyapunov stability theory to modify the desired trajectory constraint equations. A leakage-type of adaptive law is designed to compensate unknown perturbations in adaptive robust control. Finally, comparing with nominal control and control method in [32], simulation results demonstrate the superiority of trajectory stabilization control and adaptive robust control in trajectory tracking control.
Neoadjuvant Study of Niraparib in Patients With HER2-Negative, BRCA-Mutated Resectable Breast Cancer
Authors: Spring Laura , Han Hyo , Liu Minetta , Hamilton Erika , Irie Hanna , Santa-Maria Cesar , Reeves James , Pan Peng , Shan Ming , Tang Yongqiang , Graham Julie , Hazard Sebastien , Ellisen Leif , Isakoff Steven
Published: August 2021

This study evaluated neoadjuvant niraparib antitumor activity and safety in patients with localized HER2-negative, BRCA-mutated breast cancer. Twenty-one patients received niraparib 200 mg once daily in 28-day cycles. After 2 cycles, tumor response (≥30% reduction from baseline) by MRI was 90.5% and 40.0% (6/15) of patients who only received niraparib (2–6 cycles) had pathological complete response. No new safety signals were identified and high niraparib intratumoral concentration was observed.
Barriers and Facilitators to Implementation of a Telephone Follow-Up Program for Severely Injured Trauma Patients – A Process Evaluation
Authors: Wake Elizabeth , Brandenburg Caitlin , Heathcote Kathy , Dale Kate , Campbell Don , Cardona Magnolia
Published: August 2021

Background: Survival rates following major trauma are increasing. Understanding the longitudinal outcomes of major trauma can promote successful recovery. A novel, hospital-led telephone follow-up program was implemented by a multi-disciplinary clinical trauma service team at a Level I trauma centre. This evaluation was conducted to examine what factors promoted or impeded the program’s implementation.Methods:A prospective mixed methods process evaluation was conducted alongside the implementation of the follow up program. Quantitative and qualitative data were collected across the evaluation domains: reach, dose, fidelity, context and sustainability and clinical data of program participants (patients); semi-structured interviews with staff delivering the program and patients and caregivers who had participated in the program and direct observations (by researchers) of the program delivery and dataDescriptive statistics and thematic analysis were applied to quantitative and qualitative data respectively.Results:274 major trauma patients (ISS ≥ 12) were eligible for follow up. A response rate of over 75% was achieved at both follow -up timepoints, with nurses responsible for the majority of calls. Limited time and competing clinical demands were identified as barriers to the timely completion of the calls, although over 75% of calls were completed within the designated timeframe. Staff and patients valued the pre-existing trauma service/patient relationship, and this facilitated program implementation. Clinicians were motivated to evaluate their patient’s recovery, whilst patients felt ‘cared for’ and ‘not forgotten’ post-hospital discharge. Teamwork and leadership were highly valued by the clinical staff throughout the implementation period as ongoing source of motivation and support. Although primarily designed as a data collection activity, staff spontaneously developed the program to incorporate clinical follow up processes by providing guidance, advice and referrals to patients who indicated ongoing issues such as pain or emotional problems.Conclusion: Telephone follow up within a clinical trauma service team is feasible, accepted by staff and valued by patients and families. Despite time constraints, the successful implementation of this program is reliant on existing clinical/patient relationships, staff teamwork and leadership support.
Carbonic Anhydrase Isoforms of Pyropia Yezoensis: Intracellular Location and Expression Profiles in Response to Inorganic Carbon Concentration and Life Stage
Authors: Zhang Baoyu , Liu Xueying , Huan Li , Shao Zhizhuo , Zheng Zhenbing , Wang Guangce
Published: August 2021

BackgroundMacroalgae, particularly commercially grown seaweed, substantially contribute to CO2 removal and carbon storage. However, knowledge regarding the CO2 concentrating mechanism (CCM) of macroalgae is limited. Carbonic anhydrase (CA), the key component of biophysical CCM, plays important roles in many physiological reactions in various organisms. CA has been widely studied in microalgae and higher plants. However, few characteristics of CA in Pyropia yezoensis are known, particularly its intracellular location and responses to different concentrations of Ci.ResultsWe retrieved transcriptomic and genomic data for P. yezoensis and identified, amplified and characterized 11 putative genes encoding CA. The predicted corresponding proteins clustered into three subfamilies: α-, β- and γ-type. Transcriptomic and qRT-PCR results revealed different expression levels of these PyCA genes in the two life stages of P. yezoensis, and their expression levels varied under different Ci conditions. The intracellular localization of eight CA isoforms—one in the chloroplasts, four in the cytoplasm and three in the mitochondria—were elucidated with fusion proteins. One γCA isoform (PyCA10) was shown to be localized in the cytosol, and its localization is discussed. We also discuss the potential roles of the CA isoforms in development and the CCM in the two life stages. ConclusionsCA isoforms in P. yezoensis are widely distributed within cells, and higher PyCA expression, particularly of certain chloroplastic, cytosolic and mitochondrional CAs, is observed more often during the leafy stage. This expression results in stronger carbon sequestration ability in leafy thalli than filamentous thalli.
CD73 Controls Ocular Adenosine Levels and Protects Retina from Light-Induced Phototoxicity
Authors: Losenkova Karolina , Takeda Akira , Ragauskas Symantas , Kaja Simon , Paul Marius L. , Schmies Constanze C. , Rolshoven Georg , Müller Christa E. , Sandholm Jouko , Jalkanen Sirpa , Kalesnykas Giedrius , Yegutkin Gennady G
Published: August 2021

Ocular ATP and adenosine have emerged as important signalling molecules involved in vascular remodeling, retinal functioning and neurovascular coupling in the mammalian eye. However, little is known about the regulatory mechanisms of purinergic signaling in the eye. Here, we used three-dimensional multiplexed imaging in combination with in situ enzyme histochemistry, flow cytometric analysis and single cell transcriptomics to characterize the pattern of purine metabolism in the mouse and human eyes. This study identified NTPDase1/CD39 and ecto-5’-nucleotidase/CD73 as major ecto-nucleotidases, which are selectively expressed in the optic nerve head, vascular endothelial and perivascular cells, outer segments of photoreceptors, retinal microglia, and cornea and coordinately control ATP and adenosine levels. The relevance of the CD73-adenosine axis was confirmed by flash electroretinography showing that pharmacological inhibition of CD73 in dark-adapted mouse eyes rendered the animals hypersensitive to prolonged bright light, manifested as decreased a-wave and b-wave amplitudes and a loss of retinal ganglion cells. Our study thus defines ocular adenosine metabolism as a complex and spatially integrated network and characterizes its critical role in protecting the retina from light-induced phototoxicity.
Development of PLGA-Triiodothyronine Nanoparticles for Targeted Delivery in the Cardioprotection Against Ischemic Insult
Authors: Karakus Ozlem Ozen , Darwish Noureldien H. E. , Salaheldin Taher , Dickinson P. C. Taylor , Weil Brian , Mousa Shaker A
Published: August 2021

Background: Ischemic heart disease is the main cause of death globally. Cardioprotection is the process whereby mechanisms that reduce myocardial damage, and activate protective factors, contribute to the preservation of the heart. Targeting these processes could be a new strategy in the treatment of post-ischemic heart failure (HF). Triiodothyronine (T3) and thyroxine (T4), which have multiple effects on the heart, prevent myocardial damage. Results: This study describes the formulation, and characterization, of chemically modified polymeric nanoparticles incorporating T3, to target the thyroid hormone receptors. Modified T3 was conjugated to polylactide-co-glycolide (PLGA) to facilitate the active targeting of PLGA-T3. Modified T3 and PLGA-T3 was characterized with 1H-NMR. Protective role of synthesized Phosphocreatine (PCr) encapsulated PLGA-T3 nanoparticles (PLGA-T3/PCr NPs) and PLGA-T3 nanoparticles (PLGA-T3 NPs) in hypoxia-mediated cardiac cell insults were investigated. Conclusions: Data demonstrated that PLGA-T3/PCr NPs represent a potentially new therapeutic for the control of tissue damage in cardiac ischemia and resuscitation.
Bionic Biphasic Composite Scaffold with Osteochondrogenic Factors for Regeneration of Full-thickness Osteochondral Defect
Authors: Zhang Yanbo , Han Yu , Peng Yachen , Lei Jie , Chang Fei
Published: August 2021

Background: Full-thickness osteochondral defects lack the capability to self-repair owing to their complicated hierarchical structure. At present, clinical treatments including microfracture etc. have shown some efficacy; however, the newborn tissue exhibits some drawbacks, such as fibrocartilage formation and insufficient mechanical properties, eventually leading to osteoarthritis. Moreover, cartilage and bone tissues have different biological characteristics. Therefore, the simultaneous repair of full-thickness osteochondral defects is highly challenging. Methods: To achieve optimal repair, we constructed a bionic biphasic composite scaffold with on-demand osteochondrogenic factors. Kartogenin and extractive mesenchymal stem cells were introduced into a mPEG-block-poly(L-valine) thermogel set as the upper layer to accelerate cartilage repair. A poly(lactide-co-glycolide)/hydroxyapatite porous scaffold was immobilized with bone morphogenetic protein-2 as the bottom layer to facilitate subchondral repair. The intermediate interface, being a combination of the two layers was expected to reconstruct the calcification zone. Results: Gross evaluation, micro-computed tomography, and magnetic resonance imaging indicated that at 6 months after implantation, the scaffold encapsulating mesenchymal stem cells and osteochondrogenic factors had a better effect compared with other groups. More importantly, the composition of the new cartilage and bone was confirmed by western blotting, H&E staining, and immunohistochemistry. Conclusions: Taken together, this bionic composite scaffold with osteochondrogenic factors offers a promising option for the repair of full-thickness osteochondral defects.
Targeting MYC-inducing enhancer-associated noncoding (MYC-IEANC) RNAs inhibits the proliferation of HCC cells
Authors: Choi Hae In , Chai Jin Choul , Lee Young Seek , Jung Kyoung Hwa , Chai Young Gyu
Published: August 2021

Background

MYC, a critical oncogene, encodes the c-MYC transcription factor (TF) and plays an essential role in hepatocellular carcinoma (HCC) development. Recent studies have identified numerous tissue-specific enhancers of MYC in various cancers, but an HCC-specific enhancer of MYC remains elusive.
Methods

We analyzed enhancer markers, including H3K27ac enrichment and enhancer RNA (eRNA) expression, to determine putative enhancer regions of MYC in HCC cells. Enhancer activity was detected using a dual-luciferase reporter assay. We used the CRISPR-Cas9 system to edit the enhancer regions and performed antisense oligonucleotide (ASO) to inhibit eRNA. The functions of enhancers and eRNAs on HCC cells were confirmed by cell proliferation assay and sphere formation assay.
Results

We choose two active enhancers R2 and R3, with high activity among six putative enhancer regions by analyzing enhancer markers. Enhancer R2 and R3 are present approximately 800 kb downstream from the MYC gene. We confirmed eRNA activities in the enhancer regions. Depletion of these enhancer regions inhibited eRNAs significantly reduced MYC expression. In addition, MYC enhancers and eRNAs regulated HCC cell proliferation and progression.
Conclusion

In this study, we present MYC enhancers in HCC and elucidate the molecular functions of MYC-inducing enhancer-associated noncoding (MYC-IEANC) RNAs in the proliferation of HCC cells. Furthermore, our results suggest that MYC-IEANC RNAs, which play an oncogenic role in HCC cells, can be a target for HCC treatment.
Solvent Controlled Colorimetric And Fluorometric Detection of Fe2+ and Cu2+ Ions By Naphthaldimine-Glucofuranose Conjugate
Authors: Loya Mini , Dolai Bholanath , Atta Ananta Kumar
Published: August 2021

The sensing properties of naphthaldimine-glucofuranose conjugates 1 and 2 towards metal ions were investigated by 1H NMR titration, FTIR, absorbance, and fluorescence spectroscopic methods. The absorbance and fluorescence studies indicated that compound 1 formed coordination with Fe2+ and Cu2+ ions in DMSO through color changes yellow to brown and colorless, respectively. The Job's plots using absorbance data showed metal-ligand binding ratio is 1:1 for both cases. The formation of 1-Fe2+ and 1-Cu2+ complexes have been analyzed by absorption and emission spectroscopy, high-resolution mass spectrometry (HRMS) data, FTIR, 1H NMR titration experiment, and DFT calculations. The detection limits of naphthaldimine sugar conjugate 1 towards Fe2+/Cu2+ were calculated from UV-vis and fluorescence data according to the standard method. The sugar-naphthaldimine conjugate 2 has been used to establish the binding mode of 1 with Fe2+ or Cu2+ ions in DMSO.
Seismic Isolation Technology for Prestressed Segmental Precast Piers
Authors: Zou Shuang , Wenliuhan Heisha , Liu Yanhui , Yang Zhenyu
Published: August 2021

Due to its poor energy dissipation capacity, the application of prestressed segmental precast piers in bridge engineering in areas with medium- and high-intensity seismic activity is limited. This study proposes the use of a functional separation seismic isolation device (FSID) that acts as an external replaceable energy dissipation device to increase the energy dissipation capacity of prestressed segmental precast piers. Full-scale in-situ load tests on an actual prestressed segmental precast pier were carried out to clarify the seismic performance of this kind of pier. Based on the results of the tests and further analysis, an optimal design of the functional separation seismic isolation device is obtained by incorporating the optimal mechanical performance parameters of the device. The FSID proposed in this study can meet the seismic performance requirements of prestressed segmental precast piers under different levels of earthquakes.
Baseline Study of Rock Samples Collected From Paleolithic Archaeological Site of Attirampakkam, Tamil Nadu
Authors: Tamilarasi A , Chandrasekaran A , Sathish V , S Manigandan , A Lakshmi
Published: August 2021

In the present work, rock samples have been collected from Paleolithic archaeological site Attirampakkam, Tamil Nadu, India to assess the presence of mineralogical composition of samples using Fourier Transform infrared-spectroscopic (FT-IR) technique and these identified minerals are confirmed by using X-Ray Diffraction (XRD) technique. From FT-IR spectra the presence of minerals such as quartz, orthoclase, microcline, kaolinite, montmorillonite, dolomite, aragonite, and palygroskite minerals are identified in rock samples. In this samples quartz is the majorly presented mineral and crystallinity index of quartz (SiO2) is estimated for all the samples by comparing the ratio of intensity of the characteristic peak at 778 and 695 cm− 1 with the corresponding ratio for a standard sample. In rock samples, calculated crystallinity index of quartz is greater than 1 and shows that the disordered in nature. Additionally some more minerals such as hematite and rutile are identified in rock samples by X-ray diffraction technique. This extensive study shows that the archeological rock samples are wide variation in mineral composition.
Study on the Effect of Magnetic Field on Temperature Domain During Electrolytic Processing
Authors: Jia Jianli , Wang Mingzhe , Xu Tianci , Pangyan Haotian , Zhou Xueying
Published: August 2021

A physical model, mathematical model and geometric model of multi-physical field (electric field, flow field, temperature field, magnetic field) were established to explore the influence of magnetic field on the temperature domain in the gap during ECM. The change law of temperature domain of ECM gap under different magnetic field design methods was studied by using COMSOL MULTIPHYSICS. The scheme is as follows: the magnetic field line is perpendicular to the electric field and the flow field is parallel; the magnetic field line is parallel to the electric field and the flow field is vertical; the electric field of the magnetic field is vertical and the flow field is vertical. The change law of the influence of the magnetic field on the electrolyte temperature is studied by simulation. The changes of current density under three magnetic field design methods and different electrolyte flow were studied. The simulation results show that when the magnetic field is perpendicular to the electric field and the flow field, the temperature change is relatively gentle, and the flow field changes uniformly under the action of the magnetic field volume force, so that the change of current density is relatively stable; The current density of anodic dissolution increases with the increase of voltage, resulting in the increase of electrolyte temperature and heat, further reducing the gap and machining gap, and the temperature in the gap will be greatly increased. Under the action of magnetic field, the electrolyte flow rate increases and the electrolyte temperature decreases greatly.
Accurately Detection of A-Globin Gene Copy Number Variants With Two Reactions Using Droplet Digital PCR
Authors: Bao Xiuqin , Qin Danqing , Ma Jian , Zhou Xiangcheng , Wang Jicheng , Yao Cuize , Zhang Liang , Du Li
Published: August 2021

Background: α-thalassemia, with carrier rates of 11.31% and 17.55% respectively in Guangdong and Guangxi province, is a highly prevalent disease in Southern China and tropical and subtropical regions and is mainly caused by deletion in α-globin gene (HBA1 and HBA2). The clinical manifestation of α-thalassemia is highly correlated with the copy number of α-globin gene. The decrease of copy number results in α-thalassemia, while duplication or triplication compounded with β-thalassemia may aggravate the clinical manifestation. However, the usual methods we used to measure the copy number variants can only detect the three common types: --SEA, -α3.7 and − α4.2, which may easily miss the rare deletional type and duplication or triplication cases. Therefore, it is essential to establish a new method which allows detection of different copy number variants in α-globin genes, including deletion, duplication and triplication simultaneously and accurately.Methods: 428 peripheral blood and fetal chorionic villus or amniotic fluid samples were recruited in this study. We used a pair of primers and two probes to perform droplet digital PCR.Results: By performing only two reactions, we accurately detected the copy number variants in α-globin genes, including the common form α-thalassemia, triplications such as αααanti4.2 and trisomy 16. The accuracy rate for detecting the copy number of α-globin genes can up to 100%.Conclusions: In conclusion, droplet digital PCR served as an accurate and rapid method for copy number variation detection in clinical screening for α-thalassemia.