bluuebunny/crossref_metadata_2025 huggingface dataset:
Saberes, Produção Associada e bem viver: A Festa de Troca de Sementes Crioulas em comunidades tradicionais camponesas da baixada cuiabana - MT
Authors: Cabral Cristiano Apolucena , Da Cruz Burema Luana , Caetano Edson
Published: May 2020

Este texto tem como objetivo apresentar uma breve discussão acerca da existência da produção associada e agroecológica, da singular cultura do trabalho e da vivência de alguns princípios do Bem-Viver, que dão fundamento histórico para a instituição da 'Festa de troca de sementes crioulas', nas comunidades tradicionais camponesas da baixada cuiabana. Temática essa que vem sendo discutida pelo Grupo de Estudos e Pesquisas sobre Trabalho e Educação (GEPTE) do Programa de Pós-Graduação em Educação (PPGE) da Universidade Federal de Mato Grosso (UFMT). Trata-se de uma pesquisa teórica e de campo sobre o assunto em questão com análises, rodas de conversas e entrevistas.
Palavras-chave: Comunidade Tradicional Camponesa, Produção Associada, Sementes Crioulas, Solidariedade.
 
Knowledge, Associated Production and buen vivir: The Creole Seed Exchange Festival in traditional peasant communities in the Baixada Cuiabana - MT                                      
ABSTRACT. This text aims to present a brief discussion about the existence of the associated production and agro, the unique culture of work and experience of some principles of Bem-Viver, which give the historical basis for the imposition of 'Feast of exchange of seeds crioulas' in traditional communities peasants from cuiabana. This issue is being discussed by the Group of Studies and Research on Work and Education (GEPTE) of the Graduate Program in Education (PPGE) of the Federal University of Mato Grosso (UFMT). It is a theoretical research and field on the matter concerned with analysis, wheels of conversations and interviews.
Keywords: Peasant Traditional Community, Associated Production, Creole Seeds, Solidarity.
 
Saber, Producción Asociada y buen vivir: El festival de Intercambio de Semillas Criollas en comunidades campesinas tradicionales en la Baixada Cuiabana - MT
RESUMEN. Este texto tiene como objetivo presentar una breve discusión sobre la existencia de producción asociada y agroecológica, la cultura de trabajo única y la experiencia de algunos principios de Well-Living, que dan una base histórica para la institución del 'Seed Exchange Party'. Criollo ', en las comunidades campesinas tradicionales de la Baixada Cuiabana. Este tema ha sido discutido por el Grupo de Estudio e Investigación sobre Trabajo y Educación (GEPTE) del Programa de Posgrado en Educación (PPGE) de la Universidad Federal de Mato Grosso (UFMT). Esta es una investigación teórica y de campo sobre el tema en cuestión con análisis, ruedas de conversación y entrevistas.
Palabras clave: Comunidad Campesina Tradicional, Producción Asociada, Semillas Criollas, Solidaridad.
High-efficiency Non-viral Transfection of Human Primary T Lymphocytes
Authors: Khanahmad Hossein , Rahimmanesh Ilnaz , Totonchi Mehdi
Published: May 2020

The development and optimization of an effective non-viral gene delivery method for genetic manipulation of primary human T cells is a major challenge in clinical immunotherapy researches. According to the low transfection efficiency of conventional methods in human primary T cells, there is an effort in order to increase the transfection rate in these cells. Protamine is an FDA-approved compound with a documented safety profile that enhances DNA condensation for gene delivery. In this study, the effect of protamine on the transfection efficiency of standard transfection reagents, TurboFect, and Lipofectamine 2000 was evaluated in order to transfect primary human T cells. Results demonstrated that protamine condenses DNA and increases the positive charge of DNA/Cargo complex efficiently without any cytotoxic effect on the primary human T cells. The results also revealed that the DNA/Protamine/Cargo complexes effectively transfect human primary T cells.
Uma periferia, dois centros: o município de Praia Grande no contexto da formação da macrometrópole paulista no começo do século XXI
Authors: Marcos Pinto da Cunha José , Antonio Chaves de Farias Luiz , Augusto Eichman Jakob Alberto
Published: May 2020

O presente estudo tem dois objetivos principais: discutir os impactos, consequências e respostas demográficas do processo de expansão e estruturação de grandes aglomerações urbanas, com especial enfoque no caso do município de Praia Grande/SP na Região Metropolitana da Baixada Santista (RMBS); e examinar, com mais detalhes, as nuances da mobilidade populacional e, em particular, das modalidades migratórias (e suas características), como elementos importantes da peculiar inserção regional do referido município enquanto “periferia simultânea" de duas regiões metropolitanas. A partir do uso exaustivo dos dados censitários, em especial dos Censos Demográficos de 2000 e 2010, analisam-se o crescimento e a expansão territorial de Praia Grande, enfatizando, em nível intramunicipal, o papel das distintas modalidades de migração (intrametropolitana e inter-regional). Além disso, procura-se associá-las a diferentes motivaçõesa partir de um olhar detalhado para o perfil sociodemográfico dos fluxos estabelecidos. Como principais resultados do trabalho, observou-se que o papel de Praia Grande enquanto área de expansão metropolitana da RMBS se encontra lastreado pelos crescentes fluxos migratórios intrametropolitanos provenientes do polo regional, realidade esta que igualmente pode ser vista em outros contextos metropolitanos. No entanto, enquanto peculiaridade de seu processo de ocupação e inserção regional, parte majoritária dessa migração é originária de fora da RMBS, mais precisamente do município de São Paulo, centro de outra importante área de metropolitana, fato que contribui para caracterizar Praia Grande como uma “periferia com dois centros”.
HUBUNGAN PEMBELAJARAN PKn DENGAN PENGAMALAN SILA PERSATUAN INDONESIA PESERTA DIDIK SMP KEMBARAN 1 BANYUMAS
Authors: Taniredja Tukiran , Abduh Muhammad
Published: May 2020

Tujuan penelitian untuk mengetahui proses pembelajaran PKn, pengamalan sila Persatuan Indonesia, dan   hubungan pembelajaran PKn dengan pengamalan sila Persatuan Indonesia. Populasi penelitian seluruh peserta didik kelas 8F SMP Kembaran 1 Banyumas yang berjumlah 32. Seluruh populasi dijadikan sampel penelitian, sehingga merupakan sensus/sampel jenuh. Pengumpulan data dengan kuesioner tertutup. Analisis data dengan persentase dan  korelasi product moment. Hasil analisis data menunjukkan proses pembelajaran PKn kuat (78%), pengamalan Sila Persatuan Indonesia sangat kuat (82%) dan  tidak ada korelasi yang signifikan antara proses pembelajaran PKn dengan pengamalan  sila Persatuan Indonesia , rxy (0,21).

 
Kata Kunci:  pembelajaran PKn, peserta didik,  Sila Persatuan Indonesia
PENGEMBANGAN MODEL PEMBELAJARAN PROJECT CITIZEN BERORIENTASI CIVIC KNOWLEDGE, CIVIC DISPOSITION, DAN CIVIC SKILL SEBAGAI INOVASI DALAM MATA KULIAH PENDIDIKAN PKn SD
Authors: Widodo Susilo Tri , Renggani Renggani , Sukarjo Sukarjo
Published: May 2020

Penelitian ini dilakukan dengan tujuan mengembangkan model pembelajaran project citizen yang berorientasi pada civic knowledge, civic disposition, dan civic skill sebagai bentuk inovasi dalam pembelajaran mata kuliah Pendidikan PKn SD. Inovasi model pembelajaran yang dikembangkan memberikan powerful learning atau belajar yang berbobot dan bermakna yang secara pedagogis bercirikan prinsip meaningful (bermakna), integrative (terpadu ), value based (berbasis nilai), chalengging (menantang), activiting (mengaktifkan) dan joyfull (menyenangkan) Penelitan ini dilaksanakan di jurusan Pendidikan Guru Sekolah Dasar, Fakultas Ilmu Pendidikan, Universitas Negeri Semarang. Subyek penelitian ini adalah mahasiswa. Penelitian ini merupakan penelitian pendidikan dengan pendekatan pengembangan (R&D).

Hasil penelitian pengembangan yang dilakukan menunjukkan rancangan model pembelajaran layak untuk dikembangkan dengan melihat uji kelayakan dari validator yang menunjukkan 94,62%,  penilaian produk tiap bagian dan keseluruhan yang menunjukkan rata-rata kategori sangat baik. Selain itu, dilihat dari terjadinya peningkatan kefeektifan penggunaan inovasi model pembelajaran yang dikembangkan. Peningkatan tersebut dari 50,43% menjadi 89,22% sehingga secara sederhana dapat dilihat terjadi signifikansi peningkatan 38,79 %. Berdasarkan hasil penelitian tersebut menunjukan bahwa inovasi model pembelajaran tersebut efektif untuk dikembangkan dalam pembelajaran mata kuliah PKn SD

 
Kata kunci : project citizen,  pembelajaran, PKn SD
METODE PENANAMAN NILAI SOSIAL PADA SANTRI DI PONDOK PESANTREN SALAFIYAH DAN IMPLEMENTASINYA DALAM KEGIATAN MASYARAKAT PULUTAN LOR RW 02 KECAMATAN SIDOREJO KOTA SALATIGA TAHUN 2018
Authors: Jamasri Jamasri , Nusarastriya Yosaphat Haris
Published: May 2020

This study is titled the method of planting social value in santri at the Salafiyah Islamic Boarding School RW 02, Sidorejo sub-district, Salatiga city in 2018. This type of research is a qualitative descriptive study with 45 research subjects as kyai and santri. Data was collected by observation, interviews, documentation, and questionnaires. Data analysis using qualitative descriptive techniques. The results showed that the method of planting social values used was the Sorogan and Bandongan methods. The embedded social values include discipline, empathy, respect, caring, and tolerance. The implementation of the social values of santri in community activities is to become a TPA worker, a large study committee and mutual cooperation.

 

Keywords:  social values, santri, islamic boarding schools, community activities
HUBUNGAN KEMAMPUAN SISWA DALAM MENGIDENTIFIKASI KEBERAGAMAN SUKU, AGAMA, RAS, DAN ANTARGOLONGAN DALAM BINGKAI BHINNEKA TUNGGAL IKA DENGAN SIKAP MENGHARGAI KEBERAGAMAN SUKU, AGAMA, RAS, DAN ANTARGOLONGAN (STUDI SISWA KELAS VII SMP NEGERI 4 SURAKARTA)
Authors: Wahyuningsih Ika , Suryono Hassan , Rasyid Machmud Al
Published: May 2020

The purpose of this study was to determine the relationship between students' ability in identifying ethnic, religious, racial and intergroup diversity in the frame of Unity in Diversity with an attitude of respect for ethnic, religious, racial and intergroup diversity in class VII students of Surakarta State Junior High School 4.

This research is quantitative research. The method used is descriptive correlational statistics. Subjects in this study were class VII students of SMP Negeri 4 Surakarta using simple random sampling technique. Data collection techniques used are test and questionnaire instruments. Validity test used uses the product moment formula. Reliability testing uses the Spearman Brown formula to test the X variable, and the Cronbach Alpha formula to test the Y variable. Data analysis uses a simple correlation technique.

The results in this study are that there is a positive and significant relationship between students' ability to identify ethnic, religious, racial and intergroup diversity in the frame of Unity in Diversity with an attitude of respect for ethnic, religious, racial and intergroup diversity. A positive relationship between variable X and variable Y is indicated by the value of r count = 0.889> r table = 0.244. The meaning of the relationship between variable X and variable Y is significant indicated by the value of t count = 15, 28> t table1.27.

 
Keywords: identifying diversity, respect.
PENGUATAN ECOLOGICAL CITIZENSHIP SEBAGAI UPAYA MENGUBAH PERILAKU MASYARAKAT SADAR AKAN LINGKUNGAN MELALUI PROGRAM KAMPUNG SELO BERAKSI
Authors: Fatmalasari Hesti , Yuliandari Erna , Gunawati Dewi
Published: May 2020

This study aims to find out: (1) Strengthening ecological citizenship in changing the behavior of people aware of the environment through the Kampung Selo Beraksi program; (2) Obstacles faced in realizing the Kampung Selo Beraksi program. This study uses qualitative research methods and descriptive research approaches. The research technique used was purposive sampling. Data collection uses interviews, observation and document analysis. Testing data validity using data triangulation and methods. Data analysis uses an interactive analysis model which includes: data reduction, data presentation, and conclusion drawing. Stages of research include: (1) Preparation; (2) Data collection; (3) Analysis of data; (4) Preparation of research reports. The results of this study include: (1) Strengthening ecological citizenship is needed to arouse community awareness through environmental activities, namely the Kampung Selo Beraksi program. Insightful activities in the Kampung Selo Beraksi program include garbage banks, hydroponic parks, vertical gardens, craft galleries, and outbound. Strengthening ecological citizenship in the Kampung Selo Beraksi program is a bottom up approach that is realized through the formation of environmental knowledge, environmental skills, environmental attitudes, environmental participation. (2) The obstacles faced in realizing the Kampung Selo Beraksi program come from internal and external. The barriers to the Kampung Selo Beraksi program originating from the internal include: a) limited human resources due to lack of activeness and participation of the Kampung Selo community members; b) limited sources of funds in the development of the Kampung Selo Beraksi program. Barriers to the Kampung Selo Beraksi program originating from the external include: a) obstacles from the community, namely the existence of counter-attitudes from the community and a lack of community participation; b) obstacles from the government, namely lack of attention and participation of the local government; c) obstacles from the environment, namely the location of Kampung Selo Beraksi far from the city and the maximum arrangement of the village. Keywords: Strengthening, ecological citizenship, Kampung Selo
The Influence of Profession on Functional Ankle Stability in Musicians
Authors: Rein Susanne , Fabian Tobias , Zwipp Hans , Heineck Jan , Weindel Stephan
Published: May 2020

OBJECTIVE: The aim of this study was to examine the influence of extensive work-related use of the feet on functional ankle stability among musicians. METHODS: Thirty professional organists were compared to professional pianists and controls. All participants completed a questionnaire.
Range of motion (ROM), peroneal reaction time, and positional sense tests of the ankle were measured. The postural balance control was investigated with the Biodex Stability System for the stable level 8 and unstable level 2. Statistical analysis was done with the Kruskal-Wallis test, Mann-Whitney
test with Bonferroni-Holm correction, and Fisher’s exact test. RESULTS: Nine of 30 organists compared to 5 of 30 pianists and controls reported ankle sprains in their medical history. Pianists had a significant increased flexion of both ankle joints compared to organists (p≤0.01)
and increased flexion of the right ankle joint compared to controls (p=0.02). The positional sense test and postural balance control showed no significant differences among groups. The peroneal reaction time of the right peroneus longus muscle was significantly increased in pianists compared
to controls (p=0.008). CONCLUSIONS: Organists have shown a high incidence of ankle sprains. Despite their extensive work-related use of the ankle joints, organists have neither increased functional ankle stability nor increased ROM of their ankle joints in comparison to controls. Pianists
have increased flexion of the ankle joint, perhaps due to the exclusive motion of extension and flexion while using the pedals. To minimize injuries of the ankle and improve functional ankle stability as well as balance control, proprioceptive exercises of the ankle in daily training programs
are recommended.
CHARACTERIZATION OF TRACES OF PREDATION AND PARASITISM ON FOSSIL ECHINOIDS
Authors: FARRAR LYNDSEY , GRAVES ERIN , PETSIOS ELIZABETH , PORTELL ROGER W. , GRUN TOBIAS B. , KOWALEWSKI MICHAL , TYLER CARRIE L.
Published: May 2020

Interactions with predators and parasites can result in traces found on Recent and fossil echinoids. However, identifying specific trace makers, particularly on fossil echinoids, remains contentious. To document the range of trace morphologies present on echinoids and improve our ability to identify and quantify biotic interactions affecting echinoids, we characterized traces found on fossil echinoids using museum collections and field sampling spanning the Jurassic to Recent worldwide. Using light microscopy, 8,564 individual echinoid specimens were examined including 130 species, and 516 traces of potential biotic interactions identified. Morphological characteristics were recorded for each trace, including the shape of the trace outline, maximum diameter and cross-section profile. Based on shared morphological characteristics, it was possible to classify all traces into eight categories: circular, subcircular, elongated, irregular, rectangular, figure-eight, notched, and linear. Cross-section characteristics provided additional insights into the identity of potential trace makers. To further evaluate the proposed biotic origins of these traces, trace diversity was examined through time and compared with anticipated ecological trends associated with the diversification of echinoids, and their predators and parasites. Trace diversity increased over time, starting in the late Eocene, coincident with the proliferation of echinoid-drilling gastropods, an indication that biotic interactions intensified through evolutionary time, as predicted by several macroevolutionary hypotheses previously tested using mollusks. The morphological descriptions provided here enhance our understanding of biotic traces on fossil echinoids, and the potential to identify temporal trends in the intensity and diversity of biotic interactions that have affected echinoids throughout their evolutionary history.
Annelida, a Robot for Removing Hydrate and Paraffin Plugs in Offshore Flexible Lines: Development and Experimental Trials
Authors: Santos Hugo Francisco , Perondi Eduardo André , Wentz André Viegas , da Silva Junior Anselmo Luís , Barone Dante Augusto , Galassi Maurício , de Castro Bruno Barbosa , dos Reis Ney Robinson , Basso Eduardo Donadel , Pereira Pinto Hardy Leonardo , Ferreira André Manoel , Ferreira Lincoln Homero
Published: May 2020

Methane hydrates and paraffin plugs on flexible lines are concerns in offshore production. They may stop wells for months, causing high financial losses. Sometimes, operators use depressurization techniques for hydrate removal. Another strategy is using coiled tubing or a similar unit to perform local heating or solvent injection. However, frequently these strategies are not successful. In those cases, a rig may perform the operation, or the line may be lost.

We developed a robotic system to perform controlled local heating and remove obstructions. The system developed can access the line from the production platform. It uses a self-locking system to exert high traction forces. An umbilical with neutral buoyancy and low friction coefficient allows significant friction reduction. It allows moving upward and in pipes with a large number of curves. Coiled tubing and similar units cannot do that. Carbon fiber vessels and compact circuits give the flexibility to move inside 4-in. flexible pipes.

In addition, a novel theoretical model allows the cable traction calculation using an evolution of the Euler-Eytelwein equation. Experimental tests validated this model using curved pipes, both empty and filled with fluid, and using different loads. Experimental tests also confirmed the external layer traction resistance. Furthermore, the carbon fiber vessels were pressure tested, indicating a collapse resistance of 57 MPa (8,300 psi). Besides, exhaustive tests of the onboard electronics and the surface control system guarantee the communication reliability.

In addition, a theoretical model allowed the design of the 25 kN (5.6 kip) traction system considering the self-locking system, the contact with the wall, and a diameter range. Four prototypes allowed us to compare hydraulic and electric drive systems, validate the self-locking mechanism up to its limit, analyze the hydraulic system for leg opening and translation, and prove the traction capacity. Finally, a theoretical model allowed the local heating system and the temperature to increase. The experimental validation of the system on a cooled environment demonstrated its ability to increase temperature. Further, it allowed the obstruction removal in a controlled manner, avoiding damage to the polymeric layer of the flexible line.
Barriers to and facilitators of the implementation of multi-disciplinary care pathways in primary care: a systematic review
Authors: Seckler Eva , Regauer Verena , Rotter Thomas , Bauer Petra , Müller Martin
Published: May 2020

Background : Care pathways (CPWs) are complex interventions that have the potential to reduce treatment errors and optimize patient outcomes by translating evidence into local practice. To design an optimal implementation strategy, potential barriers to and facilitators of implementation must be considered. The objective of this systematic review is to identify barriers to and facilitators of the implementation of CPWs in primary care (PC). Methods : A systematic search via Cochrane Library, CINAHL, and MEDLINE via PubMed supplemented by hand searches and citation tracing was carried out. We considered articles reporting on CPWs targeting patients at least 65 years of age in outpatient settings that were written in the English or German language and were published between 2007 and 2019. We considered (non-)randomized controlled trials, controlled before-after studies, interrupted time series studies ( main project reports ) as well as associated process evaluation reports of either methodology. Two independent researchers performed the study selection; the data extraction and critical appraisal were duplicated until the point of perfect agreement between the two reviewers. Due to the heterogeneity of the included studies, a narrative synthesis was performed. Results : 14 studies (seven main project reports and seven process evaluation reports) of the identified 8,154 records in the search update were included in the synthesis. The structure and content of the interventions as well as the quality of evidence of the studies varied. The identified barriers and facilitators were classified using the Context and Implementation of Complex Interventions framework . The identified barriers were inadequate staffing, insufficient education, lack of financial compensation, low motivation and lack of time. Adequate skills and knowledge through training activities for health professionals, good multi-disciplinary communication and individual tailored interventions were identified as facilitators. Conclusions : In the implementation of CPWs in PC, a multitude of barriers and facilitators must be considered, and most of them can be modified through the careful design of intervention and implementation strategies. Furthermore, process evaluations must become a standard component of implementing CPWs to enable other projects to build upon previous experience. Trial registration : PROSPERO 2018 CRD42018087689
Refractive Outcomes following Vitrectomy and Phacovitrectomy in Rhegmatogenous Retinal Detachment
Authors: Liu Nan , Li Fuqiang , Che Songtian , Shu Zhimin , Shan Chenli , Zhao Jinsong
Published: May 2020

Background

Cataracts are commonly seen during the diagnosis of rhegmatogenous retinal detachment (RRD) or occur following its surgical management. Refractive changes following combined phacoemulsification and vitrectomy (phacovitrectomy) can affect the quality of vision. This study aimed to evaluate the refractive changes following vitrectomy versus phacovitrectomy in patients with RRD.
Methods

A total of 96 patients who underwent retinal surgery for RRD in one eye, between August 2016 and January 2018, were enrolled and divided into three groups. In 18 eyes, 23-gauge transconjunctival sutureless vitrectomy (23G TSV) was performed (group A); 25G TSV was performed in 49 eyes (group B), and phacovitrectomy with 25G TSV was performed in 29 eyes (group C). Silicone oil was injected after the surgery in all three groups. Anterior and posterior corneal astigmatism, axial length (AL), and anterior chamber depth (ACD) were measured at baseline and postoperatively at 1 week, 1 month, and 3 months.
Results

Anterior and posterior corneal astigmatism did not change, and AL continued to increase for 3 months after surgery, whereas ACD decreased postoperatively but quickly returned to baseline in groups A and B. In group C, anterior and posterior corneal astigmatism, AL, and ACD increased postoperatively, and corneal astigmatism returned to baseline by 3 months, but AL and ACD continued to increase for 3 months.
Conclusion

Vitrectomy alone does not affect anterior or posterior corneal astigmatism. Following vitrectomy, the AL increases, while the ACD decreases transiently. When vitrectomy is combined with phacoemulsification for cataract removal, the anterior and posterior corneal astigmatism increases transiently, while AL and ACD are enlarged for several months following surgery.
The mitochondrial calcium uniporter induces apoptosis in cardiomyocytes cultured with high-glucose medium by affecting mitochondrial function
Authors: Chen Xia , Guo Wenyun , Jing Zhe , Zhang Tao , Wu Zhaoqi , Cui Hengqiang , Zheng Wenxue , Chen Yongqing , Chen Hui
Published: May 2020

Background

As the number of diabetics worldwide continues to increase, diabetic cardiomyopathy has become one of the main causes of cardiovascular disease risk in diabetic patients. Currently, the pathophysiological mechanism of DCM has not been fully elucidated. In the present study, relevant pathological changes of cardiomyocytes in the high glucose environment were simulated by in vitro culture of rat H9C2 cardiomyocytes, to explore the mechanism by which MCU induces apoptosis in cardiomyocytes.
Method:

Cultured rat myocardium H9C2 cells in vitro and divided into high glucose group (glucose concentration 33 mmol/L), normal group (glucose concentration 5.5 mmol/L), experimental group (5.5 mmol/L glucose and transfected with MCU siRNA) and control group (5.5 mmol/L glucose and transfected negative control siRNA). Comparative analysis of MCU expression, Ca2+ uptake, mitochondrial function, oxidative stress and apoptosis of two groups of cells.
Results

(1) Compared with normal group, in the high glucose group the MCU expression of myocardial cells in H9C2 rats decreased, The Ca2+ levels, membrane potential and mitochondrial ATP levels decreased, mitochondrial ROS levels increased, NADH+/NADPH ratio in cardiomyocytes increased, GSH/GSSG ratio decreased, the expression levels of cleaved caspase-3 and cleaved caspase-9 increased, bcl-2 expression decreased, the number of cardiomyocytes apoptotic cells increases. (2) Compared with the normal group and the control group, the experimental group MCU expression of myocardial cells in H9C2 rats decreased, The Ca2+ levels, membrane potential and mitochondrial ATP levels decreased, mitochondrial ROS levels increased, NADH+/NADPH ratio in cardiomyocytes increased, GSH/GSSG ratio decreased, the expression levels of cleaved caspase-3 and cleaved caspase-9 increased, bcl-2 expression decreased, the number of cardiomyocytes apoptotic cells increases.
Discussion

This study suggested that MCU expression in rat H9C2 cardiomyocytes was decreased in the high glucose environment, causing abnormal mitochondrial calcium uptake and imbalanced calcium homeostasis, which may further contribute to mitochondrial dysfunction and enhanced oxidative stress in cardiomyocytes. Mitochondrial dysfunction and enhanced oxidative stress ultimately led to apoptosis in cardiomyocytes.
Clinical features of middle-aged patients died of 2019 Novel Coronavirus–Infected Pneumonia
Authors: Huang Yangyang , Zeng Guangwang , Chen Guoxi , Cai Yuyang , Huang Hai , Wang Yaling , Hou Min , Li Xiaopan , Ji Chen , Zhang Di , Lan Xing , Yang Ling
Published: May 2020

Background

Since December, 2019, the emerge of a Novel Coronavirus Disease 2019 (COVID-19) have caused global concern, the majority viewpoints are that the deaths are related to advanced ages, however, the emergence of younger deaths needs immediate attention.
Methods

Clinical characteristics, laboratory results, chest CT scans and treatment measures were retrospectively reviewed for six deceased patients under 60 years old with confirmed COVID-19 who were admitted to Wuhan Pulmonary Hospital in Wuhan, Hubei Province, China, from 6th January to 4th March, 2020.
Results

The age range of the patients was 50.17 ± 3.25 years (45–55 years), none of them had underlying diseases. All of patients presented with the symptoms of fever, respiratory system and digestive system. The level of albumin and lymphocyte counts decreased early in the stages of the disease, and then gradually increased. The neutrophil to lymphocyte ratio (NLR) increased (higher than normal range) in early stage and gradually decreased when the disease progressed. All the six patients showed ground-glass opacity (GGO) and bilateral patchy shadowing in early stage under the computed tomography (CT). All the patients died of severe pneumonia and multiple organ failure.
Conclusions

The decreased level of serum albumin occurred in the early stages of the disease, and delays in the first admission to hospital probably aggravate the disease development. The increase of the NLR in early stage of disease may be a risk of death for COVID-19. These findings may provide useful information for optimizing supportive care for COVID-19 pneumonia.
Comparative Analysis of the Mitochondrial Genome Sequences of two Medicinal Plants: Arctium Lappa and A. Tomentosum
Authors: Zhang Dachuan , Xing Yanping , Xu Liang , Zhao Rong , Yang Yanyun , Zhang Tingting , Li Shengnan , Bao Guihua , Ao Wuliji , Liu Tao , Kang Tingguo
Published: May 2020

Backguound: Mitochondrial genome sequence analysis is of great significance for understanding the evolution and genome structure of different plant species. Arctium lappa and A. tomentosum are distributed in China and frequently used as medicinal plants. People usually think A. tomentosum is an adulterant or substitute of A. lappa as a traditional Chinese Medicine (TCM). It is therefore critically important to identify the different species that are utilized in medicinal applications. This study aims to determine and compare their mitochondrial genomes, gene structure, and phylogenetic relationship. These results may provide additional insights into development of genetic research. Results: We determined the complete sequences of the mitochondrial genomes of A. lappa and A. tomentosum for the first time. The mitochondrial genomes of A. lappa and A. tomentosum were assembled into 2 single circular molecules of 312598 bp and 312609 bp, respectively. A total of 131 and 130 genes were annotated in two plants. 50 pairs of large repeat sequences were detected in A. lappa and A. tomentosum. The number of simple sequence repeats (SSRs) in both species was 192 while the total length of SSR was 2491 bp for A. lappa and 2489 bp for A. tomentosum. Only 51 single nucleotide polymorphisms (SNPs) and 3 insertion-deletions (InDels) were detected between the two plants. The two mitochondrial genome structures were highly similar and highly collinear. Both of the chloroplast genomes and mitochondrial genomes of the two plants had the phenomenon of gene exchange and transfer. Core genes and specific genes were analyzed for A. lappa and A. tomentosum and three closely related Asteraceae species, the specific gene of A. lappa was orf115a. In addition, a phylogenetic tree of the mitochondrial genomes were constructed, which laced the two Arctium species into one branch within Asteraceae. Conclusions: We identified and analyzed the mitochondrial genome features of two species of Arctium in China with implications for species identification and phylogenetic analysis. The mitochondrial genomes of A. lappa and A. tomentosum were very similar in size and structure. The ORF genes of the two were different, which could provide a theoretical basis for the development of molecular markers.
Analysis of metabolic syndrome factors to determine contributions to pathogenesis of mammary gland disease in Japanese women– Case-control study based on health screening results
Authors: Iida Tadayuki , Sumida Sakika , Masao Yoshikawa , Kaoru Nagaoka
Published: May 2020

Background: Recently, the relationship between obesity and breast cancer has been clarified, and findings obtained in a study that used body mass index (BMI) suggested that its presence might increase the risk for development of that disease. However, the association between metabolic syndrome caused by obesity and development of mammary gland disease has not been elucidated in a longitudinal manner. In the present case-control study, that association was examined.Methods: Two hundred sixty-five women who underwent breast cancer screening with mammography and measurements of metabolic syndrome factors, including waist circumference, blood glucose, and triglycerides, HDL-cholesterol levels, and systolic and diastolic blood pressure, twice within a 2-year period at the Disease Prevention Promotion and Research Center of the Dai-ikai Medical Corporation were enrolled. To determine the presence of mammary gland disease, 2 expert physicians interpreted radiogram findings, with category 3 or higher shown by mammography considered to indicate an abnormality.Results and Discussion: Waist circumference at the initial measurement was shown to be significantly associated (odds ratio=1.036, p=0.045) as a risk factor for onset of mammary gland disease. During the 2-year examination period, changes in systolic blood pressure (odds ratio=1.020, p=0.085) and diastolic pressure (odds ratio=1.040, p=0.065) showed a marginally significant association. As for protective factors, HDL-cholesterol level (odds ratio=0.982, p=0.064) at the initial examination was confirmed to have a marginally significant relationship. We concluded that waist circumference and 2-year changes in systolic and diastolic blood pressure are risk factors for mammary gland disease, while HDL-cholesterol level is a protective factor.
WITHDRAWN: Comparison of Standard Versus Accelerated Epithelium-Off and Transepithelial Corneal Cross-Linking in Pediatric Keratoconus: An Up-to-date Meta-Analysis
Authors: Li Yuanjun , Yin Yewei , Hu Tu , Du Kaixuan , Lu Ying , Fu Yanyan , Xiang Aiqun , Wu Xiaoying , Xia Xiaobo , Wen Dan
Published: May 2020

Purpose: To compare the efficacy of four corneal cross-linking (CXL) protocols: standard epithelium-off (SCXL), accelerated epithelium-off (ACXL), transepithelial (TCXL), and accelerated transepithelial (A-TCXL) for pediatric keratoconus.Methods:A comprehensive literature search on the efficacy of SCXL, ACXL, TCXL and A-TCXL in treating keratoconus patients age 18-year or under was conducted using PubMed and EMBASE up to March 2020. Primary outcomes included uncorrected visual acuity (UCVA) and maximum keratometry (Kmax). Secondary outcomes included best-corrected visual acuity (BCVA), central corneal thickness (CCT), and mean refractive spherical equivalent (MRSE). Estimations were analyzed by weighted mean difference (WMD) and 95% confidence interval (95% CI) for the outcomes during observation periods from 6 to 36 months.Results:Eight papers involving total 704 eyes were enrolled. In pediatrics, ACXL resulted in significantly better postoperative UCVA than SCXL at 12-month observation (WMD = 0.08, 95% CI: 0.02 to 0.14, P = 0.007), while SCXL provided statistically better BCVA (WMD = -0.07, 95% CI: -0.12 to -0.01, P = 0.01) and MRSE (WMD = 0.31, 95% CI: 0.06 to 0.56, P = 0.01) than ACXL at 24 months. Furthermore, SCXL provided significantly improved BCVA compared with TCXL at 12- to 24-month observation. (WMD = -0.13, 95% CI: -0.21 to -0.05, P = 0.001). Conclusions:In pediatric keratoconus, although UCVA in short-term follow-up after ACXL was better than that after SCXL, the long-run results showed that SCXL may provide superior visual acuity than either ACXL or TCXL. Further investigation is required to compare the efficacy of different CXL protocols for the management of pediatric keratoconus.
Interactive Effects of Spectral Lights and Salt Stress on Production of Secondary Metabolites and Growth Behavior in Economically Important Brassica Rapa
Authors: Khan Iftikhar Muhammad , Iqbal Javeed , Ahmad Nisar , Ali Waqar , Rehman Hina , Ali Syed Shujait , Fazal Hina , Khan Abbas , Wei Dong Qing
Published: May 2020

BackgroundEnvironmental factors like, temperature, humidity, light intensity, the supply of water, minerals, and CO2 influence the growth of a plant and biomass accumulation and production of secondary metabolites. Plant cell culture technologies have been effective tools for both studying and producing plant secondary metabolites under in vitro conditions and for plant improvement. The main objective of the current study was to investigate the biomass production and accumulation of secondary metabolites in Brassica rapa in response to synergism of abiotic factors including salt and colored lights.MethodsBrassica rapa sterilized seed were germinated and exposed to different colored lights (white as a control, yellow, blue, green and red) in combination with different concentrations of salt. The length of shoot, root and biomass (fresh and dry) were monitored during the developmental stages. Total phenolics and flavonoids content of the samples were also investigated. Data was analyzed using ANOVA-single factorial.ResultsResults revealed that plants exposed to the synergism of salt stress and spectral lights have shown negative effects on the seed germination. Shoot length (75 mm) increased, while root length (55 mm) was inhibited in plants exposed to synergism of spectral lights and different concentrations of salt when compared with controlled conditions (48 mm for shoot and 100 mm for root). However, the fresh weight of the plants was increased at lower concentration of salt than controlled. Lowest concentration of salt in synergism with green light showed the maximum response. Total phenolic content increased, while total flavonoid decreases with increasing salt concentration. Maximum total phenolic and flavonoids content were recorded in dark incubation with a high and medium concentration of salt respectively.ConclusionBased on these results, it was concluded that synergism of low salt concentration and colored lights are effective but high concentrations of salt in combination with colored lights inhibited the growth of Brassica rapa.
Interaction of light and gravity signals as a mechanism of counteracting alterations caused by simulated microgravity in proliferating plant cells
Authors: Manzano Aránzazu , Pereda-Loth Veronica , Bures Anne de , Sáez-Vásquez Julio , Herranz Raúl , Medina F. Javier
Published: May 2020

Background

Light and gravity are fundamental cues for plant development on Earth. In space, understanding the effects of changing conditions affecting to these two stimuli is key for optimizing the life support systems to come with space exploration. Simulated microgravity is useful as a complement to real spaceflight experiments into refining our knowledge of early plant development adaptation to extraterrestrial environments.
Results

In wild type Arabidopsis and in mutants of the two genes of the essential nucleolar protein nucleolin (nuc1 and nuc2), the use of an extended toolbox of cell proliferation, cell growth and ribosome biogenesis markers, has allowed us to show that the incorporation of an illumination regime, in this case photoperiod, has been sufficient to attenuate or suppress the effects caused by gravitational stress at the cellular level in the root meristem. These results are consistent with other experiments carried out at real and simulated microgravity in which early plant development is nominal when the environmental conditions are optimal (nutrients, light, temperature, humidity).
Conclusions

Light signals may total or partially replace gravity signals significantly improving plant growth and development in microgravity. Despite that, molecular alterations are still compatible with the expected adaptation mechanisms that should be better understood. The differential sensitivity of nuc1 and nuc2 mutants to gravitational stress points to new strategies to produce more resilient plants to travel with humans in new extraterrestrial endeavors.
Manual CPR Vs Mechanical CPR: Which One Is More Effective During Ambulance Transport?
Authors: Şan İshak , Bekgöz Burak , Ergin Mehmet , Usul Eren
Published: May 2020

Objectives We aimed to evaluate and compare the qualities of chest compressions performed manually by healthcare professionals and by a mechanical chest compression device on a training model during an ambulance transfer. DesignThis is an experimental trial. Setting This study was performed by the EMS of Ankara City (Capital of Turkey). 20 (10 male and 10 female) paramedic participated the study. We used LUCAS 2 as mechanical chest compression device in the study. A total of 40 rounds were driven on the track; in that moving ambulance, the model was applied chest compression in 20 rounds by paramedics, while in 20 rounds were applied by mechanical chest compression device. The depth, rate and hands-off time of chest compression were measured by means of the model's recording system. Results The median chest compression rate was 120.1 compressions per minute (IQR 25–75%=117.9–133.5) for the paramedics, whereas it was 102.3 compressions per minute for the mechanical chest compression device (IQR 25–75%=102.1–102.7) (p<0.001). The median chest compression depth was 38.9 millimeters (IQR 25–75%=32.9–45.5) for the paramedics, whereas it was 52.7 millimeters for the mechanical chest compression device (IQR 25–75%=51.8–55.0) (p<0.001). The median hands-off time during cardiopulmonary resuscitation was 6.9% (IQR 25–75=5.0–10.1%) for the paramedics and 9% (IQR 25–75%=8.2–12.5%) (p=0.09). Conclusion Chest compressions performed by the mechanical chest compression device were found to be within the range recommended by the guidelines in terms of both speed and duration.
Hygienic Food Practice among Food Workers in Jordanian Food Factories during pandemic crisis: COVID-19:comparative study
Authors: omar sharaf
Published: May 2020

Food borne disease is transmitted easily among food handlers working in food factories. This study aimed to evaluate hygienic food practice among food handlers working in food factories in Jordan during pandemic crisis: COVID-19 . A total of 500 food handlers in 25 food factories were participated in this question survey study. The knowledge of food handlers during COVID-19 pandemic was higher than their knowledge before COVID-19 pandemic with a mean percentage score of 83.4 ± 8.3%, 73.3 ± 10.8% respectively. In general, the food handlers attitude toward safe food handling during COVID-19 was higher than their attitude before COVID-19 with mean percentage score of 91.3% ± 12.7 and 88.36% ± 10.7% respectively. The awareness of food handlers in Jordanian factories for COVID-19 requirements was with a mean percentage score of 70.4% ± 14.9%. Our study showed that fair knowledge for food handlers in food safety doesn't necessary reflect their positive attitude. The overall scores for this study were 79.13% . Enforcement the governmental regulations, increasing the number of education and training courses and encourage the positive behavior food handlers would improve the status of food hygiene during COVID-19.
Identification of risk factors for the severity of coronavirus disease 2019: a retrospective study of 163 hospitalized patients
Authors: Tu Ye , Yang Ping , Wang Jingjing , Tian Xuebi , Wang Kai , Wang Chaolong , Luo Ailin , Gao Feng
Published: May 2020

Background: To compare clinical features between moderate and severe cases with COVID-19, and screen factors associated with disease severity.Methods: Demographic and clinical data were compared between moderate and severe cases. Logistic regression was performed for prognostic factors.Results: 163 patients (median age 65.0 (56.8-71.0) years, 78 (47.9%) females) were enrolled, including 87 (53.4%) severe and 76 (46.6%) moderate cases. 79 (90.8%) severe and 59 (77.6%) moderate cases had comorbidities, with hypertension and diabetes commonly presented. The most common symptoms were fever. Severe cases had higher lactate dehydrogenase (LDH), inflammatory cytokines and lymphopenia, eosinopenia on admission, and lower eosinophil and higher neutrophil counts from admission to day 13 and 19. Multivariable regression showed that neutrophilia, eosinopenia, high LDH and D-dimer were associated with severe COVID-19. In receiver operating characteristic curve analysis, LDH, eosinophil and neutrophil + eosinophil + LDH + D-dimer combination, with area under curve of 0.86, 0.76 and 0.93, predicted severe illness with high sensitivity (82.8%, 83.3%, 88.0%) and specificity (68.4%, 84.2%, 81.3%).Conclusions: Eosinopenia, higher LDH and neutrophil + eosinophil + LDH + D-dimer combination on admission were powerful indicators of severe COVID-19. Dynamic changes of neutrophils and eosinophils may be used to evaluate disease progression.
Effect of food matrix type on growth characteristics and hemolysin production of Vibrio alginolyticus
Authors: Hu Xiaojun , Wang Rundong , Deng Yijia , Deng Qi , Fang Zhijia , Sun Lijun , Wang Yaling , Gooneratne Ravi
Published: May 2020

Background: The growth and hemolysin production of V. alginolyticus at 30 °C in briny Tilapia , shrimp, scallop, oyster, pork, chicken, freshwater fish and egg fried rice were investigated. Bacterial counts were enumerated by plate counting. Hemolysin production was evaluated by blood agar and hemolytic titer tests. Results: Based on the goodness of fit primary model statistics (R 2 , MSE, BF, AF), the modified Gompertz model was a better fit to V. alginolyticus growth in foods than the logistic model. Growth kinetic parameters of V. alginolyticus displayed a higher μ max and shorter λ in briny Tilapia > shrimp > freshwater fish > egg fried rice > scallop > oyster > chicken > pork. It was notable that the V. alginolyticus counts were similar at the stationary phase, with no significant growth behavior difference between raw and cooked foods. However, higher thermostable direct hemolysin activity and hemolytic titer were observed in briny Tilapia > egg fried rice > shrimp > freshwater fish > chicken > scallop > oyster > pork. Conclusion: V. alginolyticus growth was good in all food matrix types tested. Contrary to current belief, V. alginolyticus displayed a higher hemolytic activity in some non-seafoods (freshwater fish, egg fried rice and chicken) than in scallop or oyster. This is the first report of growth and toxicity of V. alginolyticus in non-seafood. This finding will significantly improve the accuracy of microbial risk assessment of V. alginolyticus in different food matrices especially during warmer climatic periods when it is most prevalent.
Optimization Method for an Information Interaction Interface from Error-Cognition to Information Feature Mapping
Authors: Wu Xiaoli , Caldwell Sabrina
Published: May 2020

Background - With the rapid development of digital and intelligent information systems, display of visual information in interfaces has become an important challenge in the field of human-computer interaction. Objective- We propose a method for the optimization of information interaction interfaces from error-cognition through the mapping of information characteristics.Method- A mapping method of matrix description is adopted to analyze the association properties between error-cognition sets and design information sets.Results- Based on the mapping relationship between the domains of error-cognition and design information, a cross-correlational analysis is carried out between error-cognition and design information.Conclusion- We obtain the relationship matrix between the error-cognition of correlation between design information and the degree of importance among design information.Application- Taking the task interface of a warfare navigation display as an example, error factors and the features of design information are extracted. Based on the results, we also propose an optimization design scheme for the monitoring task interface.