bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and Cultural Adaptation of Psychological First Aid for COVID-19 Frontline Workers in American Indian/Alaska Native Communities
Authors: Grubin Fiona , Maudrie Tara L. , Neuner Sophie , Conrad Maisie , Waugh Emma , Barlow Allison , Coser Ashleigh , Hill Kyle , Pioche Shardai , Haroz Emily E. , O’Keefe Victoria M.
Published: July 2022

The coronavirus disease 19 (COVID-19) pandemic is broadly affecting the mental health and well-being of people around the world, and disproportionately affecting some groups with already pre-existing health inequities. Two groups at greater risk of physical and/or mental health detriments from COVID-19 and more profoundly impacted by the pandemic include frontline workers and American Indian/Alaska Native (AI/AN) communities. To provide support and prevent long-term mental health problems, we culturally adapted a psychological first aid guide specifically for COVID-19 frontline workers serving AI/AN communities. We engaged a diverse, collaborative work group to steer the adaptation content and process. We also held two focus group discussions with frontline workers in AI/AN communities to incorporate their perspectives into the adapted guide. Results from the group discussions and the collaborative work group were compiled, analyzed to extract themes and suggestions, and integrated into the adapted content of the guide. Main adaptations included updating language (i.e., to be more culturally appropriate, less prescriptive, and less text heavy), framing the guide from a harm-reduction lens, incorporating cultural activities, values, and teachings common across diverse AI/AN communities (e.g., importance of being a good relative), and validating feelings and experiences of frontline workers. The resulting adapted guide includes four modules and is available as a free online training. Our adaptation process may serve as a guiding framework for future adaptations of similar resources for specific groups. The adapted guide may stand as an enduring resource to support mental well-being, the prevention of mental health problems, and reduction of health inequities during the pandemic and beyond.
On the Existence of Greatest Elements and Maximizers
Authors: Quartieri Federico
Published: July 2022

We obtain several characterizations of the existence of greatest elements of a total preorder. The characterizations pertain to the existence of unconstrained greatest elements of a total preorder and to the existence of constrained greatest elements of a total preorder on every nonempty compact subset of its ground set. The necessary and sufficient conditions are purely topological and, in the case of constrained greatest elements, are formulated by making use of a preorder relation on the set of all topologies that can be defined on the ground set of the objective relation. Observing that every function into a totally ordered set can be naturally conceived as a total preorder, we then reformulate the mentioned characterizations in the more restrictive case of an objective function with a totally ordered codomain. The reformulations are expressed in terms of upper semi- and pseudo-continuity by showing a topological connection between the two notions of generalized continuity.
Texting Lost-to-follow-up PrEP Patients from a San Francisco Sexual Health Clinic
Authors: Johnson Kelly A. , Levy Montica , Brosnan Hannah , Kohn Robert P. , Cohen Stephanie E.
Published: July 2022

It is critical to understand what happens when PrEP patients are lost-to-follow-up (LTFU) and, where appropriate, attempt to re-engage them in care with the goal of preventing future human immunodeficiency virus (HIV) acquisition. We evaluated the benefits and limitations of using text-based outreach to re-engage with LTFU PrEP patients and offer re-initiation of PrEP care. Using text-messaging, we surveyed San Francisco City Clinic patients who started PrEP from January 2015 to October 2019 and were LTFU by October 1, 2020. Our goals were to better understand (1) whether our patients remained on PrEP through another provider or source, (2) why patients choose to discontinue PrEP, and (3) whether text-based outreach could successfully re-engage such patients in care. Multiple-choice survey questions were analyzed quantitatively to determine the proportion of respondents selecting each option; free-text responses were analyzed qualitatively using an inductive approach to identify any additional recurring themes. Of 846 eligible survey recipients, 130 responded (overall response rate 15.4%). Forty-two respondents (32.3%) were still on PrEP through another provider while 88 (67.7%) were not. Common reasons for stopping PrEP included: COVID-19–related changes in sex life (32.3% of responses), concerns regarding side effects (17.7%), and the need to take a daily pill (8.3%). Free text responses revealed additional concerns regarding risk compensation. While 32 participants agreed to be contacted by City clinic staff for PrEP counseling, only 6 were reached by phone and none of the six subsequently restarted PrEP. We learned that text messaging is a possible approach to survey certain PrEP program participants to determine who is truly LTFU and off PrEP, and to better understand reasons for PrEP discontinuation. While such information could prove valuable as programs seek to address barriers to PrEP retention, efforts to improve acceptability and increase response rates would be necessary. We were less successful in re-engaging LTFU patients in PrEP care, suggesting that text-messaging may not be the optimal strategy for this purpose.
Do Egocentric information networks influence technical efficiency of farmers? Empirical evidence from Ghana
Authors: Mohammed Sadick , Abdulai Awudu
Published: July 2022

We investigate the impact of farmers’ egocentric information network on technical efficiency and its distribution in the network, using observational data of 600 farmers from northern Ghana. We exploit community detection algorithms to endogenously identify homogeneous network communities with known structures to account for spatial heterogeneity, in a spatial stochastic frontier model that controls for social selection bias. The empirical results reveal that at the global network level, farmers’ technical efficiency strongly correlate with that of farmers in their egocentric networks. Our findings also show that farmers who are technically less efficient tend to depend on the more efficient farmers in their networks to improve efficiency. We further find that estimating spatial dependence of technical efficiency without accounting for spatial heterogeneity can lead to underestimation of technical efficiency of high (efficiency score >0.6) performing farmers, while overestimating that of medium (efficiency scores between 0.36–0.5) and low (efficiency scores between 0.1–0.35) performing farmers. The findings suggest that identifying central farmers in egocentric networks and improving their technical knowledge in a farmer-to-farmer extension organization, can contribute to improving the productivity of many farmers.
Predictors of health-related quality of life in Parkinson’s disease: the impact of overlap between health-related quality of life and clinical measures
Authors: Sanchez-Luengos Itsasne , Lucas-Jiménez Olaia , Ojeda Natalia , Peña Javier , Gómez-Esteban Juan Carlos , Gómez-Beldarrain María Ángeles , Vázquez-Picón Raquel , Foncea-Beti Nerea , Ibarretxe-Bilbao Naroa
Published: July 2022

Purpose

This study aimed to determine predictors of health-related quality of life (HRQoL) in Parkinson's disease (PD) and to explore their predictive value before and after controlling overlapping items between HRQoL and clinical variables.

Methods

One hundred and eight PD patients underwent motor, anxiety, depression, apathy, fatigue, and neurocognition assessment. HRQoL was assessed by the Parkinson’s Disease Questionnaire-39 (PDQ-39). In order to determine predictors of HRQoL in PD, stepwise multiple regression analyses were performed in two ways: before and after removing the emotional well-being dimension from PDQ-39 to control the overlap between depression and anxiety, and HRQoL.

Results

HRQoL total index was predicted by anxiety, fatigue, motor symptoms, and depression, explaining 26.9%, 7.2%, 2.8%, and 1.9% of the variance. However, after removing overlapping items, HRQoL total index was predicted by fatigue (16.5%), anxiety (6.1%), motor symptoms (3.9%), and neurocognition (2.5%), but not depression. Regarding HRQoL dimensions, mobility and activities of daily living were predicted by fatigue (19.7% and 5%) and UPDRS-III (4% and 10.2%); emotional well-being by fatigue (7.9%); social support by anxiety (12.2%) and UPDRS-III (8.6%); communication by neurocognition (5.3%) and UPDRS-III (3.4%); cognition by anxiety (10.6%) and bodily discomfort by anxiety (23%) and fatigue (4.1%).

Conclusion

These findings showed the importance of identifying and controlling overlapping items of HRQoL and clinical measures to perform an accurate interpretation. HRQoL dimensions showed different predictors before and after controlling the overlap. Based on these results fatigue, anxiety, motor symptoms, and neurocognition, but not depression are the main predictors of HRQoL in PD patients.
Three new mycoviruses identified in the apple replant disease (ARD)-associated fungus Rugonectria rugulosa
Authors: Pielhop Tom P. , Popp Carolin , Knierim Dennis , Margaria Paolo , Maiß Edgar
Published: July 2022

In this study, three new mycoviruses were identified co-infecting the apple replant disease (ARD)-associated root endophyte Rugonectria rugulosa. After dsRNA extraction, six viral fragments were visualized. Four fragments belong to a quadrivirus, which has a genome size of 17,166 bp. Each of the fragments of this quadrivirus has a single ORF encoding a protein. Two of these proteins are coat protein subunits, one ORF encodes the RdRp, and one protein has an unknown function. This virus was tentatively named rugonectria rugulosa quadrivirus 1 (RrQV1) as a member of the proposed new species Quadrivirus rugonectria. Another fragment represents the dsRNA intermediate form of a + ssRNA mitovirus with a genome size of 2410 nt. This virus encodes an RdRp and is tentatively called rugonectria rugulosa mitovirus 1 (RrMV1). RrMV1 is suggested as a member of a new species with the proposed name Mitovirus rugonectria. The sixth fragment belongs to the genome of an unclassified dsRNA virus tentatively called rugonectria rugulosa dsRNA virus 1 (RrV1). The monopartite dsRNA genome of RrV1 has a length of 8964 bp and contains two ORFs encoding a structure/gag protein and an RdRp. Full genomic sequences were determined and the genome structure as well as molecular properties are presented. After phylogenetic studies and sequence identity analyses, all three isolates are proposed as new mycoviruses. The results help to improve the understanding of the complexity of the factors involved in ARD and support the interest in mycoviral research. Subsequent analyses need to focus on the impact of mycoviruses on the biology and pathogenicity of ARD-associated fungi. The results of such studies could contribute to the development of mitigation strategies against the disease.
Could the complying with WHO physical activity recommendations improve stress, burnout syndrome, and resilience? A cross-sectional study with physical education teachers
Authors: González-Valero Gabriel , Gómez-Carmona Carlos David , Bastida-Castillo Alejandro , Corral-Pernía Juan Antonio , Zurita-Ortega Félix , Melguizo-Ibáñez Eduardo
Published: July 2022

Purpose

Teachers are exposed to inherent psychosocial risks in the workplace such as chronic stress, psychological distress, exhaustion, and burnout syndrome. To compare the values in psychosocial variables based on compliance with the recommendations for physical activity by the World Health Organization (WHO) and the type of physical activity performed.

Methods

The study had a non-experimental and comparative design, with measurements in a single group. The sample was composed of 415 physical education teachers from Spain, with an age range of 21–53 years (28.78 ± 6.15) and a heterogeneous distribution of gender (69.4% male; 30.6% women). The Perceived Stress Scale, the Maslach Burnout Inventory, the Connor–Davidson Resilience Scale, and an Ad-Hoc questionnaire were used to record the sociodemographic aspects and physical-sports practice.

Results

Most of the teachers complied with the recommendations for physical activity practice (n = 335; 80.7%). Physical activity was associated with lower signs of burnout and a greater ability to overcome. Although, teachers who did not comply with WHO recommendations, showed greater stress and emotional exhaustion. In conclusion, enough practice of physical activity based on WHO was shown as a preventive factor of stress and signs of burnout.

Conclusions

The findings suggest that perform physical activity based on the WHO recommendations helps for work stress prevention and burnout syndrome in teachers, as well as to overcome work adversities.
A model of working memory for encoding multiple items and ordered sequences exploiting the theta-gamma code
Authors: Ursino Mauro , Cesaretti Nicole , Pirazzini Gabriele
Published: July 2022

Recent experimental evidence suggests that oscillatory activity plays a pivotal role in the maintenance of information in working memory, both in rodents and humans. In particular, cross-frequency coupling between theta and gamma oscillations has been suggested as a core mechanism for multi-item memory. The aim of this work is to present an original neural network model, based on oscillating neural masses, to investigate mechanisms at the basis of working memory in different conditions. We show that this model, with different synapse values, can be used to address different problems, such as the reconstruction of an item from partial information, the maintenance of multiple items simultaneously in memory, without any sequential order, and the reconstruction of an ordered sequence starting from an initial cue. The model consists of four interconnected layers; synapses are trained using Hebbian and anti-Hebbian mechanisms, in order to synchronize features in the same items, and desynchronize features in different items. Simulations show that the trained network is able to desynchronize up to nine items without a fixed order using the gamma rhythm. Moreover, the network can replicate a sequence of items using a gamma rhythm nested inside a theta rhythm. The reduction in some parameters, mainly concerning the strength of GABAergic synapses, induce memory alterations which mimic neurological deficits. Finally, the network, isolated from the external environment (“imagination phase”) and stimulated with high uniform noise, can randomly recover sequences previously learned, and link them together by exploiting the similarity among items.
Usefulness of ethiodized oil and gelatin sponge particles for delaying the washout of indocyanine green from the liver in swine
Authors: Tanaka Ryota , Sonomura Tetsuo , Ueno Masaki , Koike Masataka , Makitani Ayano , Sato Hirotatsu , Fukuda Kodai , Higashino Nobuyuki , Ikoma Akira , Murata Shin-ichi , Minamiguchi Hiroki
Published: July 2022

Purpose

To assess the effect of ethiodized oil (EO) and gelatin sponge particles (GS) on delaying the washout of indocyanine green (ICG) from the liver in swine.

Methods

Fifteen swine were divided into 3 groups: injection of a mixture of ICG and water-soluble contrast medium (CM) followed by embolization with GS (group A), injection of a mixture of ICG and EO (group B) and injection of a mixture of ICG and EO followed by embolization with GS (group C). The liver surface was observed using an infrared camera system during and at 1, 2, 3, and 6 h after the procedure to measure ICG contrast. Livers were removed at 6 h for histopathological examination.

Results

The contrast ratio between injected and non-injected regions at 6 h was 1.45 ± 0.44 in group A, 1.89 ± 0.37 in group B, and 3.62 ± 0.76 in group C. The contrast ratio in group C was significantly greater than that in groups A and B (P = 0.032 and 0.033, respectively).

Conclusions

EO and GS delayed the washout of ICG from the liver in swine and may extend intraoperative navigation in clinical use.

Condensed abstract

Indocyanine green (ICG) mixed with ethiodized oil (EO) was injected into the left hepatic artery in swine, and the artery was embolized with gelatin sponge particles (GS). We confirmed that ICG remained in the liver parenchyma up to 6 h after the procedure. EO and GS delayed the washout of ICG from the liver in swine.
Assembly and analysis of the Populus deltoides mitochondrial genome: the first report of a multicircular mitochondrial conformation for the genus Populus
Authors: Qu Yanshu , Zhou Pengyan , Tong Chunfa , Bi Changwei , Xu Li’an
Published: July 2022

Genomics research of Populus deltoides, an important timber species that is widely planted worldwide, is an important part of poplar breeding. Currently, the nuclear and chloroplast genome of P. deltoides have been sequenced, but its mitochondrial genome (mitogenome) has not been reported. To further explore the evolution and phylogeny of P. deltoides, the mitogenome of P. deltoides I-69 was assembled using reads from Nanopore and Illumina sequencing platforms and found to consist of 802,637 bp and three circular chromosomes (336,205, 280,841, and 185,591 bp) containing 58 genes (34 protein-coding genes, 21 tRNA genes, and 3 rRNA genes). RNA analysis in combination with several species showed significantly fewer RNA editing sites in the mitogenomes of poplar and other angiosperms than in gymnosperms. Sequence transfer analysis showed extensive mitogenome rearrangements in Populus species, and with evolution from lower to higher plants, tRNA transfer from chloroplasts to mitochondria became increasingly frequent. In a phylogenetic analysis, the evolutionary status of P. deltoides was determined, and the section Populus was supported. Our results based on the first report of a multi-circular conformation of the Populus mitogenome provide a basis for further study of the evolution and genetics of P. deltoides and other Populus species and for breeding programs.
Early Postoperative Weight Loss Predicts Weight Loss up to 5 Years After Roux-En-Y Gastric Bypass, Banded Roux-En-Y Gastric Bypass, and Sleeve Gastrectomy
Authors: Tettero Onno M. , Monpellier Valerie M. , Janssen Ignace M. C. , Steenhuis Ingrid H. M. , van Stralen Maartje M.
Published: July 2022

Purpose

Previous studies showed that patients with lower weight loss after bariatric surgery could be identified based on early postoperative weight loss. However, these studies had only 12–36-month follow-up. This study aimed to explore whether patients in the lowest weight loss quartile at 3 months had lower weight loss trajectories up to 5 years after Roux-en-Y gastric bypass (RYGB), banded Roux-en-Y gastric bypass (BRYGB), and sleeve gastrectomy (SG) surgery.

Methods

Weight was assessed preoperatively, and 3, 6, 9, 12, 24, 36, 48, and 60 months postoperatively. Patients were grouped into four categories based on quartiles of percentage total weight loss (%TWL) at 3-month follow-up. Results were compared between the lowest %TWL quartile group and other quartile groups.

Results

Patients underwent either RYGB (n=13,106; 72%), SG (n=3585; 20%), or BRYGB (n=1391, 8%) surgery. Weight loss trajectories of patients in the lowest %TWL quartile group remained lower than that of other quartile groups throughout a 5-year follow-up, for all three types of surgery. Patients in the lowest %TWL quartile group had higher age at surgery, higher baseline BMI, and were more likely to be male (in the SG group), and to suffer from diabetes, hypertension, dyslipidemia, and osteoarthritis.

Conclusion

This study showed a positive association between weight loss at 3 and 12 to 60 months after bariatric surgery. Weight loss at 3 months after surgery could be used to identify patients whose anticipated weight loss trajectories are below average, to potentially improve their outcomes through early behavioral or medical interventions.

Graphical Abstract
Teacher Perceptions of Implementation Climate Related to Feasibility of Implementing Schoolwide Positive Behavior Supports and Interventions
Authors: Corbin Catherine M. , Hugh Maria L. , Ehrhart Mark G. , Locke Jill , Davis Chayna , Brown Eric C. , Cook Clayton R. , Lyon Aaron R.
Published: July 2022

For Schoolwide Positive Behavior Interventions and Supports (SWPBIS) to be effective, educators must use the practices as intended. Whether a teacher uses a practice as intended can depend, in part, on how feasible the practice is perceived to be. Identifying malleable factors associated with teachers’ perceptions of SWPBIS feasibility can help schools identify targeted supports to improve feasibility. Implementation climate, or the shared perception among implementers that their school supports implementation efforts, is known to promote high quality implementation. However, little is known about how individuals’ perceptions of their school’s implementation climate may influence their perceptions of feasibility. The lack of empirical evidence points to a need to explore whether educators’ shared and individual perceptions of implementation climate influence feasibility of implementing SWPBIS. Therefore, this study examines the link between teachers’ individual and shared perceptions of implementation climate related to the feasibility of implementing SWPBIS in a sample of 348 K-5 general education teachers across 39 elementary schools in the pacific northwestern United States. Results indicate that teachers who experience their schools’ implementation climate as positive are more likely to report SWPBIS as feasible, controlling for teachers’ shared perceptions of implementation climate. Implications for schools aiming to improve their implementation of SWPBIS, including the development of individualized implementation supports (e.g., tailoring implementation strategies to support each and every teacher), are discussed.
The Kabu-ido system and factors affecting local groundwater extraction control: case study of a customary groundwater management in Japan
Authors: Endo Takahiro
Published: July 2022

In the early 1800s, a water conflict occurred in a community named the Fukuzuka Ring Levee on the Noubi Plain, Japan. Upper villages required artesian wells for irrigation and domestic uses, but lower villages did not welcome them because drainage from the wells caused impoundment damage to their paddy fields. The Kabu-ido system was a set of rules, including regulation of the number of wells per village, introduced to de-escalate the conflict. Under the system, groundwater uses were controlled not by external authority, but by the community residents themselves. This paper has two purposes. First, it reconstructs the daily operations of the Kabu-ido system, principally by referring to surviving local diaries, to describe hitherto unknown details regarding the management of groundwater by local people 200 years ago. The diaries show that well managers, selected from residents, regulated the use of wells using various tools, including permission and surprise inspection. Second, this paper evaluates to what extent self-imposed numerical regulation was successful by checking the number of wells listed in village expenditure notes. The documents indicate that regulation did not always work. The factors underlying this are considered using the analytical framework from the commons studies. Analysis shows that, while institutional arrangements of the Kabu-ido system, such as well management with keys, rules of joint responsibility, and the prohibition of indoor wells, work positively in enforcing numerical regulation by lowering the costs of monitoring for unauthorized wells, the natural characteristics of groundwater and climate conditions such as sudden drought work negatively.
Prevention and treatment of a positive proximal margin after gastrectomy for cardia cancer
Authors: Talavera-Urquijo Eider , Davies Andrew R. , Wijnhoven Bas P. L.
Published: July 2022

A tumour-positive proximal margin (PPM) after extended gastrectomy for oesophagogastric junction (OGJ) adenocarcinoma is observed in approximately 2–20% of patients. Although a PPM is an unfavourable prognostic factor, the clinical relevance remains unclear as it may reflect poor tumour biology. This narrative review analyses the most relevant literature on PPM after gastrectomy for OGJ cancers. Awareness of the risk factors and possible measures that can be taken to reduce the risk of PPM are important. In patients with a PPM, surgical and non-surgical treatments are available but the effectiveness remains unclear.
Early and late outcomes of a series of 255 patients with Crohn’s disease who underwent resection: 10 years of experience at a single referral center
Authors: Sacchetti Franco , Caprino Paola , Potenza Angelo Eugenio , Pastena Dario , Presacco Stefano , Sofo Luigi
Published: July 2022

Patients with Crohn's disease experience an increased risk of postoperative complications and disease recurrence. The aim of this study was to investigate the role of the risk factors in determining these outcomes and whether preoperative removal of some of these risk factors would optimize the results. We conducted a retrospective study analyzing a consecutive series of 255 patients who underwent surgical resection for Crohn's disease between 2010 and 2020. We considered short- and long-term endpoints, such as postoperative complications categorized according to the Clavien–Dindo classification and the appearance of surgical and endoscopic postoperative recurrence. Univariable and multivariable analyses showed that multiple and extensive localizations increased the incidence of postoperative complications (OR = 2.19; 95% CI 1.05–4.5; p = 0.035 and OR = 1.015; 95% CI 1.003–1.028; p = 0.017 for each cm of resected segment, respectively). Regarding theoretically modifiable factors, preoperative hypoalbuminemia (for each g/L reduction) increased the risk of complications with an OR = 1.1; 95% CI 1.02–1.12; p = 0.003. Preoperative steroid therapy exerted a similar effect, with an OR = 2.6; 95% CI 1.1–5.9; p = 0.018. Modifying these last two risk factors by improving the nutritional status or discontinuing steroid therapy significantly reduced complications.

Microscopic positivity of the resection margins was a risk factor for surgical recurrence (OR = 8.7; 95% CI 1.9–40; p = 0.05). Based on the results of the present study, surgeons must examine modifiable risk factors, and careful preoperative tailored management may reduce postoperative complications and disease recurrence.
Nonlocal complement value problem for a global in time parabolic equation
Authors: Djida Jean-Daniel , Foghem Gounoue Guy Fabrice , Kouakep Tchaptchié Yannick
Published: July 2022

The overreaching goal of this paper is to investigate the existence and uniqueness of weak solution of a semilinear parabolic equation with double nonlocality in space and in time variables that naturally arises while modeling a biological nano-sensor in the chaotic dynamics of a polymer chain. In fact, the problem under consideration involves a symmetric integrodifferential operator of Lévy type and a term called the interaction potential, that depends on the time-integral of the solution over the entire interval of solving the problem. Owing to the Galerkin approximation, the existence and uniqueness of a weak solution of the nonlocal complement value problem is proven for small time under fair conditions on the interaction potential.
Performance evaluation of emerging block copolymer membranes for oil-water separation
Authors: Al-Maas Mashael , Hussain Altaf , Minier-Matar Joel , Hassan Mohammad K. , Al-Maadeed Mariam Al Ali , Alamgir Karim , Adham Samer
Published: July 2022

Membrane processes such as microfiltration (MF) and ultrafiltration (UF) are known to be the best advanced technologies for water reuse application. Numerous research efforts have been conducted in areas of modifying commercial MF/UF products or synthesizing novel materials promising enhanced oil-water separation performances. Block copolymer (BCP)-based membranes have recently gained increased popularity due to their improved water permeabilities. This study applies a comprehensive testing protocol for performance evaluation of two emerging poly (styrene-block-methyl methacrylate) BCP membranes developed by the project team. Tests mimicking industrial conditions were conducted by using a representative synthetic produced water and operating repeat tests. Both BCP membranes (referred to as A & B) were found to possess high permeabilities of 5538 and 12,424 LMH/bar, respectively. Membrane B showed higher organic rejection at 79% against 74% rejection obtained for membrane A. The novel membranes were then compared to a relevant commercial product. Lower permeability at 3831 LMH/bar and slightly higher rejection performance (within ~ 10%) were obtained for the commercial membrane as compared to the BCP membranes. Test results obtained for those novel membranes being still in the development stage will be utilized in future studies investigating further optimization of the membrane structure and oil-water separation performance.

Graphical Abstract
Intranasal Fentanyl for On-the-Hill Analgesia by Ski Patrol
Authors: Lynch Tierra V. , Callas Peter W. , Peterson Timothy D. , Schlein Sarah M.
Published: July 2022

Introduction

Intranasal fentanyl offers a means for safe and effective pain management in austere environments. Prehospital analgesia traditionally involves intravenous or intramuscular medication. However, for wilderness rescuers, these methods are often impractical.

Methods

We conducted a retrospective review of health records to evaluate the safety and efficacy of intranasal fentanyl administered by EMT-Basic certified ski patrollers. Our primary aim was to measure the reduction in initial pain scores to subsequent measurements at 5, 10, and 15 min using the pain numeric rating scale (0–10). Clinically significant reduction in severe pain has been established as ≥1.8 points. We used paired t-tests and multilevel modeling to measure statistical significance and potential interactions and reviewed patient charts for adverse events, including respiratory depression or the use of naloxone.

Results

We compiled the results from the winter seasons for 2007 through 2012 and 2016 through 2020. A total of 247 patients were included. The initial pain score was 8.6±1.5 (mean±SD). The decrease in pain scores from 0 to 5, 10, and 15 min, respectively, was –1.8, –2.4, and –2.9 ( P<0.0001), which demonstrated a clinically and statistically significant decrease in pain scores. There were no adverse events.

Conclusions

Traditional standard of care analgesics are invasive, elongate scene times, and increase the risk of environmental exposure and provider needlestick. Intranasal fentanyl offers a safe, noninvasive, and rapid analgesia that is well-suited for austere winter environments, such as those encountered at ski resorts. This study demonstrates the safety and efficacy of the administration of intranasal fentanyl by EMT-Basic certified providers.
Divine Inspiration in Byzantium
Authors: Krause Karin
Published: July 2022

In this volume, Karin Krause examines conceptions of divine inspiration and authenticity in the religious literature and visual arts of Byzantium. During antiquity and the medieval era, “inspiration” encompassed a range of ideas regarding the divine contribution to the creation of holy texts, icons, and other material objects by human beings. Krause traces the origins of the notion of divine inspiration in the Jewish and polytheistic cultures of the ancient Mediterranean and Near Eastern worlds and their reception in Byzantine religious culture. Exploring how conceptions of authenticity are employed in Eastern Orthodox Christianity to claim religious authority, she analyzes texts in a range of genres, as well as images in different media, including manuscript illumination, icons, and mosaics. Her interdisciplinary study demonstrates the pivotal role that claims to the divine inspiration of religious literature and art played in the construction of Byzantine cultural identity.
The Restraint of the Press in England, 1660-1715
Authors: Barber Alex W.
Published: July 2022

A discussion of the fascinating interplay between communication, politics and religion in early modern England, suggesting a new framework for the politics of print culture.
The Foundations of Royal Power in Early Medieval Germany
Authors: Bachrach David S.
Published: July 2022

The Ottonians were the most powerful monarchs in Europe during the tenth and early eleventh century, exercising hegemony in West Francia, Burgundy, and much of Italy in addition to ruling the German realm. Despite their enormous political and military success, however, the foundations of Ottonian royal power remain highly contested and largely misunderstood, with previous scholarship tending to have considered it as depending upon the ability of the king to shape and harness the power of the nobles.

This study challenges the dominant historiographical paradigm, rebutting the notion of putative power-sharing between the king and the nobility, which simply did not exist as a legal class in the Ottonian century. Rather, it argues that the foundations of royal power under the Ottonians comprised not only their own enormous wealth, but also their unique authority and ability, through the royal ‘bannum’ the authority inherent in the office of the king, to make use of the economic resources and labour of the broad free population of the realm, as well as from the Church. In so doing, the Ottonians drew upon and further developed the administrative, institutional, and ideological inheritance of their Carolingian predecessors, in the process creating the dominant polity in tenth-century Europe.
Memories of Tiananmen
Authors: Lee Francis , Chan Joseph Man
Published: July 2022

This book analyzes how collective memory regarding the 1989 Beijing student movement and the Tiananmen crackdown was produced, contested, sustained, and transformed in Hong Kong between 1989 and 2019. Drawing on data gathered through multiple sources such as news reports, digital media content, vigil onsite surveys, population surveys, and in-depth interviews with activists, rally participants, and other stakeholders, it identifies six key processes in the dynamics of social remembering: memory formation, memory mobilization, memory institutionalization, intergenerational transfer, memory repair, and memory balkanization. Memories of Tiananmen demonstrates how a socially dominant collective memory, even one the state finds politically irritable, can be generated and maintained through constant negotiation and efforts by a wide range of actors. While the book mainly focuses on the interplay between political changes and Tiananmen commemoration in the historical period within which the society enjoyed a significant degree of civil liberties, it also discusses how the trajectory of the collective memory may take a drastic turn as Hong Kong's autonomy is abridged. The book promises to be a key reference for anyone interested in collective memory studies, social movement research, political communication, and China and Hong Kong studies.
From City Space to Cyberspace
Authors: Wasielewski Amanda
Published: July 2022

The narrative of the birth of internet culture often focuses on the achievements of American entrepreneurs in Silicon Valley, but there is an alternative history of internet pioneers in Europe who developed their own model of network culture in the early 1990s. Drawing from their experiences in the leftist and anarchist movements of the ‘80s, they built DIY networks that give us a glimpse into what internet culture could have been if it were in the hands of squatters, hackers, punks, artists, and activists. In the Dutch scene, the early internet was intimately tied to the aesthetics and politics of squatting. Untethered from profit motives, these artists and activists aimed to create a decentralized tool that would democratize culture and promote open and free exchange of information.
Simulated Lunar Surface Hydration Measurements Using Multispectral Lidar at 3 µm
Authors: Cremons D. R. , Honniball C. I.
Published: July 2022

Accurately measuring the variability of spectroscopic signatures of hydration (H2O + OH) on the illuminated lunar surface at 3 μm as a function of latitude, lunar time of day, and composition is crucial to determining the generation and destruction mechanisms of OH species and understanding the global water cycle. A prime complication in analysis of the spectroscopic feature is the accurate removal of thermal emission, which can modify or even eliminate the hydration feature depending on the data processing methods used and assumptions made. An orbital multispectral lidar, with laser illumination at key diagnostic wavelengths, would provide uniform, zero‐phase geometry, complete latitude and time of day coverage from a circular polar orbit, and is agnostic to the thermal state of the surface. We have performed measurement simulations of a four‐wavelength multispectral lidar using spectral mixtures of hydrated mid‐ocean‐ridge basalt (MORB) glasses and lunar regolith endmembers to assess the lidar performance in measuring hydration signatures on the lunar surface. Our results show a feasible system with wavelengths at 1.5 μm, 2.65 μm, 2.8 μm, and 3.1 μm can measure lunar hydration with a precision of 52 ppm (1σ) or better. These results, combined with the uniform measurement capabilities of multispectral lidar make it a valuable spectroscopic technique for elucidating mechanisms of OH/H2O generation, migration, and destruction.
Flipping the Sequence of Martian Formation
Authors: Benningfield Damond
Published: July 2022

Analysis of the Chassigny meteorite suggests the planet acquired most of its interior volatiles from meteorites, not from the solar nebula.
Volcanic Lava Lake Belts Out Its Secrets in Seismic “Songs”
Authors: Wight Andrew
Published: July 2022

A cacophony of magma displacements and volcanic gases recorded underneath Kīlauea’s roiling lake of lava could one day provide information to help predict future eruptions.
Comprehensive assessment of actionable genomic alterations in primary colorectal carcinoma using targeted next-generation sequencing
Authors: Jan Yi-Hua , Tan Kien Thiam , Chen Shu-Jen , Yip Timothy Tak Chun , Lu Cu tai , Lam Alfred King-yin
Published: July 2022

Background

The clinical utility of comprehensive genomic profiling (CGP) for guiding treatment has gradually become the standard-of-care procedure for colorectal carcinoma (CRC). Here, we comprehensively assess emerging targeted therapy biomarkers using CGP in primary CRC.

Methods

A total of 575 primary CRCs were sequenced by ACTOnco® assay for genomic alterations, tumour mutational burden (TMB), and microsatellite instability (MSI).

Results

Eighteen percent of patients were detected as MSI-High (MSI-H), and the remaining cases were classified as microsatellite stable (MSS). Driver mutation prevalence in MSS CRCs were APC (74%), TP53 (67%), KRAS (47%), PIK3CA (21%) and BRAF (13%). The median TMBs for MSI-H and MSS patients were 37.8 mutations per mega base (mut/Mb) and 3.9 mut/Mb, respectively. Forty-seven percent of MSI-H CRC harboured at least one loss-of-function mutations in genes that may hamper immune checkpoint blockade. Among MSS RAS/RAF wild-type CRCs, 59% had at least one actionable mutation that may compromise the efficacy of anti-EGFR therapy. For late-stage CRC, 51% of patients are eligible for standard care actionability and the remaining 49% could be enrolled in clinical trials with investigational drugs.

Conclusions

This study highlights the essential role of CGP for identifying rational targeted therapy options in CRC.
ATM rules neurodevelopment and glutamatergic transmission in the hippocampus but not in the cortex
Authors: Focchi Elisa , Cambria Clara , Pizzamiglio Lara , Murru Luca , Pelucchi Silvia , D’Andrea Laura , Piazza Silvano , Mattioni Lorenzo , Passafaro Maria , Marcello Elena , Provenzano Giovanni , Antonucci Flavia
Published: July 2022

Interest in the function of ataxia-telangiectasia-mutated protein (ATM) is extensively growing as evidenced by preclinical studies that continuously link ATM with new intracellular pathways. Here, we exploited Atm+/− and Atm−/− mice and demonstrate that cognitive defects are rescued by the delivery of the antidepressant Fluoxetine (Fluox). Fluox increases levels of the chloride intruder NKCC1 exclusively at hippocampal level suggesting an ATM context-specificity. A deeper investigation of synaptic composition unveils increased Gluk-1 and Gluk-5 subunit-containing kainate receptors (KARs) levels in the hippocampus, but not in the cortex, of Atm+/− and Atm−/− mice. Analysis of postsynaptic fractions and confocal studies indicates that KARs are presynaptic while in vitro and ex vivo electrophysiology that are fully active. These changes are (i) linked to KCC2 activity, as the KCC2 blockade in Atm+/− developing neurons results in reduced KARs levels and (ii) developmental regulated. Indeed, the pharmacological inhibition of ATM kinase in adults produces different changes as identified by RNA-seq investigation. Our data display how ATM affects both inhibitory and excitatory neurotransmission, extending its role to a variety of neurological and psychiatric disorders.
KIT promotes tumor stroma formation and counteracts tumor-suppressive TGFβ signaling in colorectal cancer
Authors: Küçükköse Emre , Peters Niek A. , Ubink Inge , van Keulen Veere A. M. , Daghighian Roxanna , Verheem André , Laoukili Jamila , Kranenburg Onno
Published: July 2022

Expression profiling has identified four consensus molecular subtypes (CMS1-4) in colorectal cancer (CRC). The receptor tyrosine kinase KIT has been associated with the most aggressive subtype, CMS4. However, it is unclear whether, and how, KIT contributes to the aggressive features of CMS4 CRC. Here, we employed genome-editing technologies in patient-derived organoids (PDOs) to study KIT function in CRC in vitro and in vivo. CRISPR-Cas9-mediated deletion of the KIT gene caused a partial mesenchymal-to-epithelial phenotype switch and a strong reduction of intra-tumor stromal content. Vice versa, overexpression of KIT caused a partial epithelial-to-mesenchymal phenotype switch, a strong increase of intra-tumor stromal content, and high expression of TGFβ1. Surprisingly, the levels of phosphorylated SMAD2 were significantly lower in KIT-expressing versus KIT-deficient tumor cells. In vitro analyses showed that TGFβ signaling in PDOs limits their regenerative capacity. Overexpression of KIT prevented tumor-suppressive TGFβ signaling, while KIT deletion sensitized PDOs to TGFβ-mediated growth inhibition. Mechanistically, we found that KIT expression caused a strong reduction in the expression of SMAD2, a central mediator of canonical TGFβ signaling. We propose that KIT induces a pro-fibrotic tumor microenvironment by stimulating TGFβ expression, and protects the tumor cells from tumor-suppressive TGFβ signaling by inhibiting SMAD2 expression.
The CIP2A-TOPBP1 complex safeguards chromosomal stability during mitosis
Authors: De Marco Zompit Mara , Esteban Mònica Torres , Mooser Clémence , Adam Salomé , Rossi Silvia Emma , Jeanrenaud Alain , Leimbacher Pia-Amata , Fink Daniel , Shorrocks Ann-Marie K. , Blackford Andrew N. , Durocher Daniel , Stucki Manuel
Published: July 2022

The accurate repair of DNA double-strand breaks (DSBs), highly toxic DNA lesions, is crucial for genome integrity and is tightly regulated during the cell cycle. In mitosis, cells inactivate DSB repair in favor of a tethering mechanism that stabilizes broken chromosomes until they are repaired in the subsequent cell cycle phases. How this is achieved mechanistically is not yet understood, but the adaptor protein TOPBP1 is critically implicated in this process. Here, we identify CIP2A as a TOPBP1-interacting protein that regulates TOPBP1 localization specifically in mitosis. Cells lacking CIP2A display increased radio-sensitivity, micronuclei formation and chromosomal instability. CIP2A is actively exported from the cell nucleus in interphase but, upon nuclear envelope breakdown at the onset of mitosis, gains access to chromatin where it forms a complex with MDC1 and TOPBP1 to promote TOPBP1 recruitment to sites of mitotic DSBs. Collectively, our data uncover CIP2A-TOPBP1 as a mitosis-specific genome maintenance complex.
Nanoparticle elasticity affects systemic circulation lifetime by modulating adsorption of apolipoprotein A-I in corona formation
Authors: Li Mingyang , Jin Xinyang , Liu Tao , Fan Feng , Gao Feng , Chai Shuang , Yang Lihua
Published: July 2022

Nanoparticle elasticity is crucial in nanoparticles’ physiological fate, but how this occurs is largely unknown. Using core-shell nanoparticles with a same PEGylated lipid bilayer shell yet cores differing in elasticity (45 kPa – 760 MPa) as models, we isolate the effects of nanoparticle elasticity from those of other physiochemical parameters and, using mouse models, observe a non-monotonic relationship of systemic circulation lifetime versus nanoparticle elasticity. Incubating our nanoparticles in mouse plasma provides protein coronas varying non-monotonically in composition depending on nanoparticle elasticity. Particularly, apolipoprotein A-I (ApoA1) is the only protein whose relative abundance in corona strongly correlates with our nanoparticles’ blood clearance lifetime. Notably, similar results are observed when above nanoparticles’ PEGylated lipid bilayer shell is changed to be non-PEGylated. This work unveils the mechanisms by which nanoparticle elasticity affects nanoparticles’ physiological fate and suggests nanoparticle elasticity as a readily tunable parameter in future rational exploiting of protein corona.
Compounds activating VCP D1 ATPase enhance both autophagic and proteasomal neurotoxic protein clearance
Authors: Wrobel Lidia , Hill Sandra M. , Djajadikerta Alvin , Fernandez-Estevez Marian , Karabiyik Cansu , Ashkenazi Avraham , Barratt Victoria J. , Stamatakou Eleanna , Gunnarsson Anders , Rasmusson Timothy , Miele Eric W. , Beaton Nigel , Bruderer Roland , Feng Yuehan , Reiter Lukas , Castaldi M. Paola , Jarvis Rebecca , Tan Keith , Bürli Roland W. , Rubinsztein David C.
Published: July 2022

Enhancing the removal of aggregate-prone toxic proteins is a rational therapeutic strategy for a number of neurodegenerative diseases, especially Huntington’s disease and various spinocerebellar ataxias. Ideally, such approaches should preferentially clear the mutant/misfolded species, while having minimal impact on the stability of wild-type/normally-folded proteins. Furthermore, activation of both ubiquitin-proteasome and autophagy-lysosome routes may be advantageous, as this would allow effective clearance of both monomeric and oligomeric species, the latter which are inaccessible to the proteasome. Here we find that compounds that activate the D1 ATPase activity of VCP/p97 fulfill these requirements. Such effects are seen with small molecule VCP activators like SMER28, which activate autophagosome biogenesis by enhancing interactions of PI3K complex components to increase PI(3)P production, and also accelerate VCP-dependent proteasomal clearance of such substrates. Thus, this mode of VCP activation may be a very attractive target for many neurodegenerative diseases.