bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthesis of Metarhizium anisopliae–Chitosan Nanoparticles and Their Pathogenicity against Plutella xylostella (Linnaeus)
Authors: Wu Jianhui , Du Cailian , Zhang Jieming , Yang Bo , Cuthbertson Andrew G. S. , Ali Shaukat
Published: December 2021

Nanotechnology is increasingly being used in areas of pesticide production and pest management. This study reports the isolation and virulence of a new Metarhizium anisopliae isolate SM036, along with the synthesis and characterization of M. anisopliae–chitosan nanoparticles followed by studies on the efficacy of nanoparticles against Plutella xylostella. The newly identified strain proved pathogenic to P. xylostella under laboratory conditions. The characterization of M. anisopliae–chitosan nanoparticles through different analytical techniques showed the successful synthesis of nanoparticles. SEM and HRTEM images confirmed the synthesis of spherical-shaped nanoparticles; X-ray diffractogram showed strong peaks between 2θ values of 16–30°; and atomic force microscopy (AFM) analysis revealed a particle size of 75.83 nm for M. anisopliae–chitosan nanoparticles, respectively. The bioassay studies demonstrated that different concentrations of M. anisopliae–chitosan nanoparticles were highly effective against second instar P. xylostella under laboratory and semi-field conditions. These findings suggest that M. anisopliae–chitosan nanoparticles can potentially be used in biorational P. xylostella management programs.
Capture and Ex-Situ Analysis of Environmental Biofilms in Livestock Buildings
Authors: Guéneau Virgile , Rodiles Ana , Piard Jean-Christophe , Frayssinet Bastien , Castex Mathieu , Plateau-Gonthier Julia , Briandet Romain
Published: December 2021

Little information about biofilm microbial communities on the surface of livestock buildings is available yet. While these spatially organized communities proliferate in close contact with animals and can harbor undesirable microorganisms, no standardized methods have been described to sample them non-destructively. We propose a reproducible coupon-based capture method associated with a set of complementary ex-situ analysis tools to describe the major features of those communities. To demonstrate the biofilm dynamics in a pig farm building, we analyzed the coupons on polymeric and metallic materials, as representative of these environments, over 4 weeks. Confocal laser scanning microscopy (CLSM) revealed a rapid coverage of the coupons with a thick layer of biological material and the existence of dispersed clusters of active metabolic microorganisms. After detaching the cells from the coupons, counts to quantify the CFU/cm2 were done with high reproducibility. High-throughput sequencing of the 16S rRNA V3-V4 region shows bacterial diversity profiles in accordance with reported bacteria diversity in pig intestinal ecosystems and reveals differences between materials. The coupon-based methodology allows us to deepen our knowledge on biofilm structure and composition on the surface of a pig farm and opens the door for application in different types of livestock buildings.
Three-Layer PdO/CuWO4/CuO System for Hydrogen Gas Sensing with Reduced Humidity Interference
Authors: Kumar Nirmal , Haviar Stanislav , Zeman Petr
Published: December 2021

The growing hydrogen industry is stimulating an ongoing search for new materials not only for hydrogen production or storage but also for hydrogen sensing. These materials have to be sensitive to hydrogen, but additionally, their synthesis should be compatible with the microcircuit industry to enable seamless integration into various devices. In addition, the interference of air humidity remains an issue for hydrogen sensing materials. We approach these challenges using conventional reactive sputter deposition. Using three consequential processes, we synthesized multilayer structures. A basic two-layer system composed of a base layer of cupric oxide (CuO) overlayered with a nanostructured copper tungstate (CuWO4) exhibits higher sensitivity than individual materials. This is explained by the formation of microscopic heterojunctions. The addition of a third layer of palladium oxide (PdO) in forms of thin film and particles resulted in a reduction in humidity interference. As a result, a sensing three-layer system working at 150 °C with an equalized response in dry/humid air was developed.
A Study of 5G Edge-Central Core Network Split Options
Authors: Corici Marius , Chakraborty Pousali , Magedanz Thomas
Published: December 2021

With the wide adoption of edge compute infrastructures, an opportunity has arisen to deploy part of the functionality at the edge of the network to enable a localized connectivity service. This development is also supported by the adoption of “on-premises” local 5G networks addressing the needs of different vertical industries and by new standardized infrastructure services such as Mobile Edge Computing (MEC). This article introduces a comprehensive set of deployment options for the 5G network and its network management, complementing MEC with the connectivity service and addressing different classes of use cases and applications. We have also practically implemented and tested the newly introduced options in the form of slices within a standard-based testbed. Our performed validation proved their feasibility and gave a realistic perspective on their impact. The qualitative assessment of the connectivity service gives a comprehensive overview on which solution would be viable to be deployed for each vertical market and for each large-scale operator situation, making a step forward towards automated distributed 5G deployments.
Methylenetetrahydrofolate (MTHFR), the One-Carbon Cycle, and Cardiovascular Risks
Authors: Raghubeer Shanel , Matsha Tandi E.
Published: December 2021

The 5-10-methylenetetrahydrofolate reductase (MTHFR) enzyme is vital for cellular homeostasis due to its key functions in the one-carbon cycle, which include methionine and folate metabolism and protein, DNA, and RNA synthesis. The enzyme is responsible for maintaining methionine and homocysteine (Hcy) balance to prevent cellular dysfunction. Polymorphisms in the MTHFR gene, especially C677T, have been associated with various diseases, including cardiovascular diseases (CVDs), cancer, inflammatory conditions, diabetes, and vascular disorders. The C677T MTHFR polymorphism is thought to be the most common cause of elevated Hcy levels, which is considered an independent risk factor for CVD. This polymorphism results in an amino acid change from alanine to valine, which prevents optimal functioning of the enzyme at temperatures above 37 °C. Many studies have been conducted to determine whether there is an association between the C677T polymorphism and increased risk for CVD. There is much evidence in favour of this association, while several studies have concluded that the polymorphism cannot be used to predict CVD development or progression. This review discusses current research regarding the C677T polymorphism and its relationship with CVD, inflammation, diabetes, and epigenetic regulation and compares the evidence provided for and against the association with CVD.
A Generation Shift in Mediterranean Diet Adherence and Its Association with Biological Markers and Health in Dalmatia, Croatia
Authors: Šarac Jelena , Havaš Auguštin Dubravka , Lovrić Mario , Stryeck Sarah , Šunić Iva , Novokmet Natalija , Missoni Saša
Published: December 2021

Previous studies have confirmed the beneficial effect of a Mediterranean diet in mitigating health issues related to cardiovascular disease, diabetes and obesity. However, rapid changes in the traditional way of life and the “westernization” of the diet in Mediterranean populations, especially in younger generations, has led to progressive abandonment of healthy dietary patterns. In order to investigate the generation shift in dietary patterns and lifestyle habits in the Mediterranean part of Croatia, we compared two cohorts of 610 women (266 pregnant and 344 non-pregnant) from the same region, but from different age groups. The MDSS score was derived from food frequency questionnaires. The results showed that the young, reproductively active generation (pregnant women) in Dalmatia, Croatia, although having a higher education and socioeconomic status, exhibits a more adverse eating behaviour (lower adherence to the Mediterranean diet) and lifestyle (excessive smoking in pregnancy) than the older population from the same region. Lower MDSS scores across aggregated age groups in both cohorts showed significant association with higher blood lipid levels and higher smoking frequency. In conclusion, Mediterranean diet adherence is associated with biological markers (age, lipid profile) and lifestyle (smoking) in our study, with a more adverse trend observed in the younger generation.
Nutritional Status and Serum Levels of Micronutrients in an Elderly Group Who Participate in the Program for Complementary Food in Older People (PACAM) from the Metropolitan Region, Santiago de Chile
Authors: Arazo-Rusindo Migdalia Caridad , Zúñiga Rommy N. , Cortés-Segovia Pablo , Benavides-Valenzuela Sergio , Pérez-Bravo Francisco , Castillo-Valenzuela Oscar , Mariotti-Celis María Salomé
Published: December 2021

The increase in the Chilean elderly population has promoted public policies to favor an adequate nutrition in later life. This study evaluated the nutritional status, micronutrients intake and serum micronutrients levels of an elderly group beneficiary of the PACAM from the Metropolitan Region, Santiago de Chile. Anthropometric and dietary survey (24 h food recalls) were assessed in 182 elderly individuals (60 and 80 years old). Blood serum collection was used to measure the micronutrient status. The sample was comprised by 12.6%, 46.1%, 28.0% and 13.2% of underweight, normal weight, overweight and obese subjects, respectively. Women presented 11% of underweight, 45% of normal weight and 44% of overweight and obese, while men—18%, 50% and 32%, respectively. Only the 63% of the elderlies consumed PACAM foods, reaching average daily intakes below (50%) the recommended daily serving. Serum deficiencies of 25-hydroxyvitamin D (88%), vitamin B12 (33%) and calcium (36%) were observed, being the highest ones in the PACAM foods women (60–75 years old). Chilean elderlies presented mainly a normal weight; however, an important proportion of overweight/obese subjects was observed. Although PACAM foods consumption significantly increased the micronutrient intake, it was not enough to ensure an adequate serum micronutrient levels in the elderly.
Use of Dietary Supplements and Perceived Knowledge among Adults Living with Fibromyalgia in Norway: A Cross-Sectional Study
Authors: Kvæl Linda Aimée Hartford , Løchting Ida , Molin Marianne
Published: December 2021

Fibromyalgia syndrome (FMS) is a complex medical condition characterized by widespread musculoskeletal pain. To date, no gold standard treatment has been developed, and persons with FMS often seek alternative methods to control their symptoms, such as dietary supplements (DS). This study aimed to describe the use of DS in persons living with FMS and examine the associations between the use of DS and its potential predictors. We recruited a convenience sample of 504 participants (≥18 years) living with FMS. The main outcome variables included estimated expenditure on DS in the last 12 months in Norwegian kroner (NOK) and the differences between the groups of users and non-users of DS. Of the 504 participants, 430 reported having used DS, and the mean amount of money spent in the previous year was determined to be NOK 2300. The most common DS reported were vitamin D, magnesium, and omega-3 fatty acids. The predictors of being a DS user were high education, high self-reported knowledge of DS but low overall knowledge of health claims. Users of DS marketed for muscles/joints appear to spend more money on DS. The increasing availability of DS and aggressive advertising in the media through health claims stipulate the need for interventions that lead to informed decisions about DS.
Interventions That Successfully Reduced Adults Salt Intake—A Systematic Review
Authors: Silva-Santos Tânia , Moreira Pedro , Rodrigues Micaela , Padrão Patrícia , Pinho Olívia , Norton Pedro , Ndrio Altin , Gonçalves Carla
Published: December 2021

Background: Adequate sodium intake is important for lowering blood pressure and thus reducing cardiovascular disease risk and other complications. The aim of this review is to identify recent interventions around the world that have been successful in reducing salt intake. Methods: A search in the PubMed, Web of Science and Scopus databases was performed. We include studies published in the last 10 years; randomized trials, pilot intervention without a control arm or experimental study; adult participants; and interventions that successfully reduced salt intake. Study quality was assessed. Results: We included 21 studies, 16 randomized intervention trials and five nonrandomized intervention studies. Eleven interventions described health and nutritional education, seven interventions described nutritional education plus other interventions, and three studies used salt meters to reduce sodium intake. Conclusion: Health and nutritional education, nutritional education plus other interventions and estimates of salt intake showed success in the reduction of salt consumption. There is no evidence that one type of intervention analyzed is more effective than other in reducing salt consumption, so we must analyze each in which individuals or subpopulations will have the intervention performed and use the most suitable approaches to lead to better results.
Pharmacological Activity of Garcinia indica (Kokum): An Updated Review
Authors: Lim Sung Ho , Lee Ho Seon , Lee Chang Hoon , Choi Chang-Ik
Published: December 2021

Garcinia indica (commonly known as kokum), belonging to the Clusiaceae family (mangosteen family), is a tropical evergreen tree distributed in certain regions of India. It has been used in culinary and industrial applications for a variety of purposes, including acidulant in curries, pickles, health drinks, wine, and butter. In particular, G. indica has been used in traditional medicine to treat inflammation, dermatitis, and diarrhea, and to promote digestion. According to several studies, various phytochemicals such as garcinol, hydroxycitric acid (HCA), cyanidin-3-sambubioside, and cyanidin-3-glucoside were isolated from G. indica, and their pharmacological activities were published. This review highlights recent updates on the various pharmacological activities of G. indica. These studies reported that G. indica has antioxidant, anti-obesity, anti-arthritic, anti-inflammatory, antibacterial, hepatoprotective, cardioprotective, antidepressant and anxiolytic effects both in vitro and in vivo. These findings, together with previously published reports of pharmacological activity of various components isolated from G. indica, suggest its potential as a promising therapeutic agent to prevent various diseases.
Limitations and Challenges in the Stability of Cysteamine Eye Drop Compounded Formulations
Authors: Martín-Sabroso Cristina , Alonso-González Mario , Fernández-Carballido Ana , Aparicio-Blanco Juan , Córdoba-Díaz Damián , Navarro-García Federico , Córdoba-Díaz Manuel , Torres-Suárez Ana
Published: December 2021

Accumulation of cystine crystals in the cornea of patients suffering from cystinosis is considered pathognomonic and can lead to severe ocular complications. Cysteamine eye drop compounded formulations, commonly prepared by hospital pharmacy services, are meant to diminish the build-up of corneal cystine crystals. The objective of this work was to analyze whether the shelf life proposed for six formulations prepared following different protocols used in hospital pharmacies is adequate to guarantee the quality and efficacy of cysteamine eye drops. The long-term and in-use stabilities of these preparations were studied using different parameters: content of cysteamine and its main degradation product cystamine; appearance, color and odor; pH and viscosity; and microbiological analysis. The results obtained show that degradation of cysteamine was between 20% and 50% after one month of storage in the long-term stability study and between 35% and 60% in the in-use study. These data confirm that cysteamine is a very unstable molecule in aqueous solution, the presence of oxygen being the main degradation factor. Saturation with nitrogen gas of the solutions offers a means of reducing cysteamine degradation. Overall, all the formulae studied presented high instability at the end of their shelf life, suggesting that their clinical efficacy might be dramatically compromised.
The TKI Era in Chronic Leukemias
Authors: De Novellis Danilo , Cacace Fabiana , Caprioli Valeria , Wierda William G. , Mahadeo Kris M. , Tambaro Francesco Paolo
Published: December 2021

Tyrosine kinases are proteins involved in physiological cell functions including proliferation, differentiation, and survival. However, the dysregulation of tyrosine kinase pathways occurs in malignancy, including hematological leukemias such as chronic myeloid leukemia (CML) and chronic lymphocytic leukemia (CLL). Particularly, the fusion oncoprotein BCR-ABL1 in CML and the B-cell receptor (BCR) signaling pathway in CLL are critical for leukemogenesis. Therapeutic management of these two hematological conditions was fundamentally changed in recent years, making the role of conventional chemotherapy nearly obsolete. The first, second, and third generation inhibitors (imatinib, dasatinib, nilotinib, bosutinib, and ponatinib) of BCR-ABL1 and the allosteric inhibitor asciminib showed deep genetic and molecular remission rates in CML, leading to the evaluation of treatment discontinuation in prospective trials. The irreversible BTK inhibitors (ibrutinib, acalabrutinib, zanubrutinib, tirabrutinib, and spebrutinib) covalently bind to the C481 amino acid of BTK. The reversible BTK inhibitor pirtobrutinib has a different binding site, overcoming resistance associated with mutations at C481. The PI3K inhibitors (idelalisib and duvelisib) are also effective in CLL but are currently less used because of their toxicity profiles. These tyrosine kinase inhibitors are well-tolerated, do have some associated in-class side effects that are manageable, and have remarkably improved outcomes for patients with hematologic malignancies.
Combined Curcumin and Lansoprazole-Loaded Bioactive Solid Self-Nanoemulsifying Drug Delivery Systems (Bio-SSNEDDS)
Authors: Alshadidi Abdulrahman , Shahba Ahmad Abdul-Wahhab , Sales Ibrahim , Rashid Md Abdur , Kazi Mohsin
Published: December 2021

Background: The current study aimed to design a novel combination of lansoprazole (LNS) and curcumin (CUR) solid oral dosage form using bioactive self-nanoemulsifying drug delivery systems (Bio-SSNEDDS). Methods: Liquid SNEDDS were prepared using the lipid-excipients: Imwitor988 (cosurfactant), Kolliphor El (surfactant), the bioactive black seed (BSO) and/or zanthoxylum rhetsa seed oils (ZRO). Liquid SNEDDS were loaded with CUR and LNS, then solidified using commercially available (uncured) and processed (cured) Neusilin® US2 (NUS2) adsorbent. A novel UHPLC method was validated to simultaneously quantify CUR and LNS in lipid-based formulations. The liquid SNEDDS were characterized in terms of self-emulsification, droplet size and zeta-potential measurements. The solidified SNEDDS were characterized by differential scanning calorimetry (DSC), X-ray powder diffraction (XRD), scanning electron microscopy (SEM), in vitro dissolution and stability in accelerated storage conditions. Results: Liquid SNEDDS containing BSO produced a transparent appearance and ultra-fine droplet size (14 nm) upon aqueous dilution. The solidified SNEDDS using cured and uncured NUS2 showed complete solidification with no particle agglomeration. DSC and XRD confirmed the conversion of crystalline CUR and LNS to the amorphous form in all solid SNEDDS samples. SEM images showed that CUR/LNS-SNEDDS were relatively spherical and regular in shape. The optimized solid SNEDDS showed higher percent of cumulative release as compared to the pure drugs. Curing NUS2 with 10% PVP led to significant enhancement of CUR and LNS dissolution efficiencies (up to 1.82- and 2.75-fold, respectively) compared to uncured NUS2-based solid SNEDDS. These findings could be attributed to the significant (50%) reduction in the micropore area% in cured NUS2 which reflects blocking very small pores allowing more space for the self-emulsification process to take place in the larger-size pores. Solid SNEDDS showed significant enhancement of liquid SNEDDS stability after 6 months storage in accelerated conditions. Conclusions: The developed Bio-SSNEDDS of CUR and LNS using processed NUS2 could be used as a potential combination therapy to improve the treatment of peptic ulcers.
Polymer-Templated Durable and Hydrophobic Nanostructures for Hydrogen Gas Sensing Applications
Authors: Hossain Mohammad Kamal , Drmosh Qasem Ahmed
Published: December 2021

A simple and hands-on one-step process has been implemented to fabricate polymer-templated hydrophobic nanostructures as hydrogen gas sensing platforms. Topographic measurements have confirmed irregular hills and dips of various dimensions that are responsible for creating air bubble pockets that satisfy the Cassie–Baxter state of hydrophobicity. High-resolution field-emission scanning electron microscopy (FESEM) has revealed double-layer structures consisting of fine microscopic flower-like structures of nanoscale petals on the top of base nanostructures. Wetting contact angle (WCA) measurements further revealed the contact angle to be ~142.0° ± 10.0°. Such hydrophobic nanostructures were expected to provide a platform for gas-sensing materials of a higher surface area. From this direction, a very thin layer of palladium, ca. 100 nm of thickness, was sputtered. Thereafter, further topographic and WCA measurements were carried out. FESEM micrographs revealed that microscopic flower-like structures of nanoscale petals remained intact. A sessile drop test reconfirmed a WCA of as high as ~130.0° ± 10.0°. Due to the inherent features of hydrophobic nanostructures, a wider surface area was expected that can be useful for higher target gas adsorption sites. In this context, a customized sensing facility was set up, and H2 gas sensing performance was carried out. The surface nanostructures were found to be very stable and durable over the course of a year and beyond. A polymer-based hydrophobic gas-sensing platform as investigated in this study will play a dual role in hydrophobicity as well as superior gas-sensing characteristics.
Enhanced Performance of Cyclopentadithiophene-Based Donor-Acceptor-Type Semiconducting Copolymer Transistors Obtained by a Wire Bar-Coating Method
Authors: Kim Doyeon , Yoon Minho , Lee Jiyoul
Published: December 2021

Herein, we report the fabrications of high-performance polymer field-effect transistors (PFETs) with wire bar-coated semiconducting polymer film as an active layer. For an active semiconducting material of the PFETs, we employed cyclopentadithiophene-alt-benzothiadiazole (CDT-BTZ) that is a D-A-type-conjugated copolymer consisting of a repeated electron-donating unit and an electron-accepting unit, and the other two CDT-based D-A-type copolymer analogues are cyclopentadithiophene-alt-fluorinated-benzothiadiazole (CDT-FBTZ) and cyclopentadithiophene-alt-thiadiazolopyridine (CDT-PTZ). The linear field-effect mobility values obtained from the transfer curve of the PFETs fabricated with the spin-coating were 0.04 cm2/Vs, 0.16 cm2/Vs, and 0.31 cm2/Vs, for CDT-BTZ, CDT-FBTZ, and CDT-PTZ, respectively, while the mobility values measured from the PFETs with the wire bar-coated CDT-BTZ film, CDT-FBTZ film, and CDT-PTZ film were 0.16 cm2/Vs, 0.28 cm2/Vs, and 0.95 cm2/Vs, respectively, which are about 2 to 4 times higher values than those of the PFETs with spin-coated films. These results revealed that the aligned molecular chain is beneficial for the D-A-type semiconducting copolymer even though the charge transport in the D-A-type semiconducting copolymer is known to be less critical to the degree of disorder in film.
Towards Real-Time In-Situ Mid-Infrared Spectroscopic Ellipsometry in Polymer Processing
Authors: Ebner Alexander , Zimmerleiter Robert , Hingerl Kurt , Brandstetter Markus
Published: December 2021

Recent developments in mid-infrared (MIR) spectroscopic ellipsometry enabled by quantum cascade lasers (QCLs) have resulted in a drastic improvement in signal-to-noise ratio compared to conventional thermal emitter based instrumentation. Thus, it was possible to reduce the acquisition time for high-resolution broadband ellipsometric spectra from multiple hours to less than 1 s. This opens up new possibilities for real-time in-situ ellipsometry in polymer processing. To highlight these evolving capabilities, we demonstrate the benefits of a QCL based MIR ellipsometer by investigating single and multilayered polymer films. The molecular structure and reorientation of a 2.5 µm thin biaxially oriented polyethylene terephthalate film is monitored during a stretching process lasting 24.5 s to illustrate the perspective of ellipsometric measurements in dynamic processes. In addition, a polyethylene/ethylene vinyl alcohol/polyethylene multilayer film is investigated at a continuously varying angle of incidence (0∘– 50∘) in 17.2 s, highlighting an unprecedented sample throughput for the technique of varying angle spectroscopic ellipsometry in the MIR spectral range. The obtained results underline the superior spectral and temporal resolution of QCL ellipsometry and qualify this technique as a suitable method for advanced in-situ monitoring in polymer processing.
A Review of the Recent Progress in the Development of Nanocomposites Based on Poly(ether-block-amide) Copolymers as Membranes for CO2 Separation
Authors: Clarizia Gabriele , Bernardo Paola
Published: December 2021

An inspiring challenge for membrane scientists is to exceed the current materials’ performance while keeping the intrinsic processability of the polymers. Nanocomposites, as mixed-matrix membranes, represent a practicable response to this strongly felt need, since they combine the superior properties of inorganic fillers with the easy handling of the polymers. In the global strategy of containing the greenhouse effect by pursuing a model of sustainable growth, separations involving CO2 are some of the most pressing topics due to their implications in flue gas emission and natural gas upgrading. For this purpose, Pebax copolymers are being actively studied by virtue of a macromolecular structure that comprises specific groups that are capable of interacting with CO2, facilitating its transport with respect to other gas species. Interestingly, these copolymers show a high versatility in the incorporation of nanofillers, as proved by the large number of papers describing nanocomposite membranes based on Pebax for the separation of CO2. Since the field is advancing fast, this review will focus on the most recent progress (from the last 5 years), in order to provide the most up-to-date overview in this area. The most recent approaches for developing Pebax-based mixed-matrix membranes will be discussed, evidencing the most promising filler materials and analyzing the key-factors and the main aspects that are relevant in terms of achieving the best effectiveness of these multifaceted membranes for the development of innovative devices.
Setting Relationships between Structure and Devulcanization of Ground Tire Rubber and Their Effect on Self-Healing Elastomers
Authors: Alonso Pastor Luis E. , Núñez Carrero Karina C. , Araujo-Morera Javier , Hernández Santana Marianella , Pastor José María
Published: December 2021

The use of devulcanized tire powder as an effective reinforcement in self-healing styrene-butadiene rubber (SBR) compounds has been investigated for the first time in this work. For this purpose, the evolution of the microstructure of the rubber from end-of-life tires (ELTs) was studied during granulation, grinding and devulcanization through an exhaustive characterization work in order to relate the final microstructure with the mechanical response of the repaired systems. Different morphologies (particle size distribution and specific surface area) obtained by cryogenic and water jet grinding processes, as well as different devulcanization techniques (thermo-mechanical, microwave, and thermo-chemical), were analyzed. The results demonstrated the key influence of the morphology of the ground tire rubber (GTR) on the obtained devulcanized products (dGTR). The predictions of the Horikx curves regarding the selectivity of the applied devulcanization processes were validated, thereby; a model of the microstructure of these materials was defined. This model made it possible to relate the morphology of GTR and dGTR with their activity as reinforcement in self-healing formulations. In this sense, higher specific surface area and percentage of free surface polymeric chains resulted in better mechanical performance and more effective healing. Such a strategy enabled an overall healing efficiency of more than 80% in terms of a real mechanical recovery (tensile strength and elongation at break), when adding 30 phr of dGTR. These results open a great opportunity to find the desired balance between the mechanical properties before and after self-repair, thus providing a high technological valorization to waste tires.
Biomimetic Gradient Scaffolds Containing Hyaluronic Acid and Sr/Zn Folates for Osteochondral Tissue Engineering
Authors: Asensio Gerardo , Benito-Garzón Lorena , Ramírez-Jiménez Rosa Ana , Guadilla Yasmina , Gonzalez-Rubio Julian , Abradelo Cristina , Parra Juan , Martín-López María Rocío , Aguilar María Rosa , Vázquez-Lasa Blanca , Rojo Luis
Published: December 2021

Regenerative therapies based on tissue engineering are becoming the most promising alternative for the treatment of osteoarthritis and rheumatoid arthritis. However, regeneration of full-thickness articular osteochondral defects that reproduces the complexity of native cartilage and osteochondral interface still remains challenging. Hence, in this work, we present the fabrication, physic-chemical characterization, and in vitro and in vivo evaluation of biomimetic hierarchical scaffolds that mimic both the spatial organization and composition of cartilage and the osteochondral interface. The scaffold is composed of a composite porous support obtained by cryopolymerization of poly(ethylene glycol) dimethacrylate (PEGDMA) in the presence of biodegradable poly(D,L-lactide-co-glycolide) (PLGA), bioactive tricalcium phosphate β-TCP and the bone promoting strontium folate (SrFO), with a gradient biomimetic photo-polymerized methacrylated hyaluronic acid (HAMA) based hydrogel containing the bioactive zinc folic acid derivative (ZnFO). Microscopical analysis of hierarchical scaffolds showed an open interconnected porous open microstructure and the in vitro behaviour results indicated high swelling capacity with a sustained degradation rate. In vitro release studies during 3 weeks indicated the sustained leaching of bioactive compounds, i.e., Sr2+, Zn2+ and folic acid, within a biologically active range without negative effects on human osteoblast cells (hOBs) and human articular cartilage cells (hACs) cultures. In vitro co-cultures of hOBs and hACs revealed guided cell colonization and proliferation according to the matrix microstructure and composition. In vivo rabbit-condyle experiments in a critical-sized defect model showed the ability of the biomimetic scaffold to promote the regeneration of cartilage-like tissue over the scaffold and neoformation of osteochondral tissue.
A Scientometric Assessment of Agri-Food Technology for Research Activity and Productivity
Authors: Thavorn Jakkrit , Muangsin Veera , Gowanit Chupun , Muangsin Nongnuj
Published: December 2021

In accordance with the UN Sustainable Development Goals (SDGs), several SDGs target global food issues, including zero hunger (food security and sustainable agriculture), responsible consumption and production (food losses), climate action (greenhouse gas emissions from food waste), and partnerships for the goals (research collaboration). As such, it is vital to identify technology and market opportunities to support advanced development by exploring scientific and technological research on such SDGs. The significance of technological innovation and evaluations of activity, productivity, and collaboration aids and guides future research streams. Motivated by the growing severity of the global food waste crisis, this paper focuses on the case study of shelf-life extension technology for food and applies a scientometric analysis of patents based on text mining. VantagePoint was used to analyze 2516 patents issued between 2000 and 2020, with the aim of understanding the conceptual structure of knowledge and the social relationships among key players. The results indicate that the technology is experiencing a period of growth, and it can be clustered into five technology sectors. Across all technology clusters, China outperformed other countries in terms of the number of patents. Almost all of China’s patents applied for technology commercialization domestically, whereas other countries tended to apply for patents overseas to exploit opportunities. The findings have implications for both policymaking and strategic decision-making using a multi-layered network innovation system.
Luminescence Sensitivity of Rhine Valley Loess: Indicators of Source Variability?
Authors: Fitzsimmons Kathryn E. , Perić Zoran , Nowatzki Maike , Lindauer Susanne , Vinnepand Mathias , Prud’homme Charlotte , Dave Aditi K. , Vött Andreas , Fischer Peter
Published: December 2021

Loess provides a valuable terrestrial record of past environmental conditions, including the dynamics and trajectories of air mass circulation responsible for dust transport. Here we explore variations in the luminescence sensitivity characteristics of sedimentary quartz and feldspar as possible tools for identifying changes in source down a loess-palaeosol sequence (LPS). Luminescence sensitivity is a rapidly measurable index which is the product of interplay between source lithology and the history of the quartz or feldspar clasts. Variations in sensitivity of down profile may therefore reflect changes in sediment provenance as well as other factors such as weathering through pedogenesis. We undertake an empirical investigation of the luminescence sensitivity of quartz and feldspar from different grain-size fractions from the Schwalbenberg LPS in the German Rhine valley. We compare samples from a 30 m core spanning the last full glacial cycle with samples of oxygen isotope stage (OIS) 3–2 age exposed within nearby profile. We find an overall inverse relationship between quartz and feldspar sensitivity, as well as variability in sensitivity between different quartz grain sizes. Statistical analyses yield a significant correlation between IR50 sensitivity from unprocessed sediments and clay content, and feldspar sensitivity and Si/Al ratios down the core. Since Si/Al ratios may indicate changes in provenance, the latter correlation suggests that IR50 measurements on unprocessed samples may be used to provide a reliable, rapid scan of source variability over millennial timescales.
Tropospheric Attenuation in GeoSurf Satellite Constellations
Authors: Matricciani Emilio , Riva Carlo , Luini Lorenzo
Published: December 2021

In GeoSurf satellite constellations, any transmitter/receiver, wherever it is located, is linked to a satellite with zenith paths. We have studied the tropospheric attenuation predicted for some reference sites (Canberra, Holmdel, Pasadena, Robledo, and Spino d’Adda), which also set the meridian along which we have considered sites with latitudes ranging between 60° N and 60° S. At the annual probability of 1% of an average year, in the latitude between 30° N and 30° S, there are no significant differences between GEO slant paths and GeoSurf zenith paths. On the contrary, at 0.1% and 0.01% annual probabilities, large differences are found for latitudes greater than 30° N or 30° S. For comparing the tropospheric attenuation in GeoSurf paths with that expected in LEO highly variable slant paths, we have considered, as reference, a LEO satellite constellation orbiting in circular at 817 km. GeoSurf zenith paths “gain” several dBs compared to LEO slant paths. The more static total clear-sky attenuation (water vapor, oxygen, and clouds) in both GEO and LEO slant paths shows larger values than GeoSurf zenith paths. Both for rain and clear-sky attenuations, Northern and Southern Hemispheres show significant differences.
Deep Learning-Based Object Detection System for Identifying Weeds Using UAS Imagery
Authors: Etienne Aaron , Ahmad Aanis , Aggarwal Varun , Saraswat Dharmendra
Published: December 2021

Current methods of broadcast herbicide application cause a negative environmental and economic impact. Computer vision methods, specifically those related to object detection, have been reported to aid in site-specific weed management procedures for targeted herbicide application within a field. However, a major challenge to developing a weed detection system is the requirement for a properly annotated database to differentiate between weeds and crops under field conditions. This research involved creating an annotated database of 374 red, green, and blue (RGB) color images organized into monocot and dicot weed classes. The images were acquired from corn and soybean research plots located in north-central Indiana using an unmanned aerial system (UAS) flown at 30 and 10 m heights above ground level (AGL). A total of 25,560 individual weed instances were manually annotated. The annotated database consisted of four different subsets (Training Image Sets 1–4) to train the You Only Look Once version 3 (YOLOv3) deep learning model for five separate experiments. The best results were observed with Training Image Set 4, consisting of images acquired at 10 m AGL. For monocot and dicot weeds, respectively, an average precision (AP) score of 91.48 % and 86.13% was observed at a 25% IoU threshold (AP @ T = 0.25), as well as 63.37% and 45.13% at a 50% IoU threshold (AP @ T = 0.5). This research has demonstrated a need to develop large, annotated weed databases to evaluate deep learning models for weed identification under field conditions. It also affirms the findings of other limited research studies utilizing object detection for weed identification under field conditions.
Evaluation of Network RTK Positioning Performance Based on BDS-3 New Signal System
Authors: Wang Pengxu , Liu Hui , Yang Zhixin , Shu Bao , Xu Xintong , Nie Guigen
Published: December 2021

The BeiDou navigation satellite system (BDS-3) has been deployed and provides positioning, navigation, and timing (PNT) services for users all over the world. On the basis of BDS-2 system signals, BDS-3 adds B1C, B2a, B2b, and other signals to realize compatibility and interoperability with other global navigation satellite systems (GNSS). Network real-time kinematic (RTK) technology is an important real-time regional high-precision GNSS positioning technology. Combined with the network RTK high-precision service platform software developed by the author’s research group and the measured data of a provincial continuously operating reference station (CORS) in Hubei, this paper preliminarily evaluates the network RTK service performance under the new signal system of BDS-3. The results show that single BDS-3 adopts the new signal combination (B1C+B2a) and transition signal combination (B1I+B3I) when providing virtual reference station (VRS) services, the RTK fixation rate of the terminal is above 95%, and the horizontal and elevation accuracies are within 1cm and 2 cm, respectively, which meets the requirements of providing high-precision network RTK services by a single BDS-3 system. In addition, the positioning accuracy of BDS-2 is relatively poor, while the accuracy of BDS-3 is better than global positioning systems (GPS) and BDS-2. The combined processing effect of the B1I+B3I transition signal of BDS-2/3 is optimal, the accuracy of E and N directions is better than 0.5 cm, and the accuracy of U direction is better than 1.5 cm. It can be seen from the atmosphere correction accuracy, regional error modeling accuracy, and network RTK terminal positioning accuracy that the service effect of the B1C+B2a combination is slightly better than that of the B1I+B3I combination. When a single BDS-3 constellation provides network RTK services, it is recommended to use the B1C+B2a combination as the main frequency solution, and when the BDS-2/3 constellation provides service, it is recommended to use the B1I+B3I combination as the main frequency solution.
Evaluation and Analysis of Dust Storm Activity in Tianwen-1 Landing Area Based on the Moderate Resolution Imaging Camera Observations and Mars Daily Global Maps
Authors: Qu Shaojie , Li Bo , Zhang Jiang , Wang Yi , Li Chenfan , Zhu Yuzhou , Ling Zongcheng , Chen Shengbo
Published: December 2021

The first Mars exploration mission from China (Tianwen-1) was launched on 23 July 2020 with the goal of “orbiting, landing, and roving”. The occurrence of dust storm activities is an important criterion of assessing atmospheric risk for the Tianwen-1 landing process. Dust storm activities from Mars Year (MY) 24 to MY32 in southern Utopia Planitia were identified. Most dust storms only appeared in one Mars Daily Global Map (MDGM), with a lifetime of less than or equal to solar longitude (Ls) = 0.5°. Only if the lifetime of a dust storm is greater than or equal to Ls = 1° can it reach the primary landing ellipse. From Ls = 0–50°, dust storms are mostly in the diffusion stage with a maximum speed of movement of 2479 km/Ls. Then, the speed gradually decreases to the minimum value of 368 km/Ls when the dust storm is in the dissipation stage. If a dust storm moves at an average speed of 750 km/Ls, the safe landing zone is a circle within a radius of 750 km centered on the primary landing ellipse. From March to May 2021, eight dust storms were identified in the Moderate Resolution Imaging Camera (MoRIC) mosaics. Because there was no dust storm activity in MoRIC mosaic on 13 May 2021, we concluded that there would be no dust storm in the primary landing ellipse on 15 May (MY36, Ls = 45.1°). Therefore, the landing time of the Tianwen-1 probe was finally determined as 15 May, which successfully landed in the south of the Utopia Planitia, and the in-situ investigation was carried out by the Zhurong Mars rover.
Single Epoch Ambiguity Resolution of Small-Scale CORS with Multi-Frequency GNSS
Authors: Ji Shengyue , Zheng Qianli , Weng Duojie , Chen Wu , Wang Zhenjie , He Kaifei
Published: December 2021

The network real-time kinematic (RTK) technique uses continuously operating reference stations (CORS) within a geographic area to model the distance dependent errors, allowing users in the area to solve ambiguities. A key step in network RTK is to fix ambiguities between multiple reference stations. When a new satellite rises or when maintenance happens, many unknown parameters are involved in the mathematical model, and traditional methods take some time to estimate the integer ambiguities reliably. The purpose of this study is the single-epoch ambiguity resolution on small-scale CORS network with inter-station distance of around 50 km. A new differencing scheme is developed to explore the full potential of multi-frequency Global Navigation Satellite System (GNSS). In this scheme, a differencing operation is formed between satellites with the closest mapping functions. With the new differencing scheme, tropospheric error can be mostly neglected after the correction, as well as the double-differencing operation. Numerical tests based on two baselines of 49 km and 35 km show that the success rate of ambiguity resolution can reach more than 90%. The single-epoch ambiguity resolution for reference stations brings many benefits to the network RTK service, for example, the instantaneous recovery after maintenance or when a new satellite rises.
Autonomous System for Lake Ice Monitoring
Authors: Aslamov Ilya , Kirillin Georgiy , Makarov Mikhail , Kucher Konstantin , Gnatovsky Ruslan , Granin Nikolay
Published: December 2021

Continuous monitoring of ice cover belongs to the key tasks of modern climate research, providing up-to-date information on climate change in cold regions. While a strong advance in ice monitoring worldwide has been provided by the recent development of remote sensing methods, quantification of seasonal ice cover is impossible without on-site autonomous measurements of the mass and heat budget. In the present study, we propose an autonomous monitoring system for continuous in situ measuring of vertical temperature distribution in the near-ice air, the ice strata and the under-ice water layer for several months with simultaneous records of solar radiation incoming at the lake surface and passing through the snow and ice covers as well as snow and ice thicknesses. The use of modern miniature analog and digital sensors made it possible to make a compact, energy efficient measurement system with high precision and spatial resolution and characterized by easy deployment and transportation. In particular, the high resolution of the ice thickness probe of 0.05 mm allows to resolve the fine-scale processes occurring in low-flow environments, such as freshwater lakes. Several systems were tested in numerous studies in Lake Baikal and demonstrated a high reliability in deriving the ice heat balance components during ice-covered periods.
A Review on Computer Aided Diagnosis of Acute Brain Stroke
Authors: Inamdar Mahesh Anil , Raghavendra Udupi , Gudigar Anjan , Chakole Yashas , Hegde Ajay , Menon Girish R. , Barua Prabal , Palmer Elizabeth Emma , Cheong Kang Hao , Chan Wai Yee , Ciaccio Edward J. , Acharya U. Rajendra
Published: December 2021

Amongst the most common causes of death globally, stroke is one of top three affecting over 100 million people worldwide annually. There are two classes of stroke, namely ischemic stroke (due to impairment of blood supply, accounting for ~70% of all strokes) and hemorrhagic stroke (due to bleeding), both of which can result, if untreated, in permanently damaged brain tissue. The discovery that the affected brain tissue (i.e., ‘ischemic penumbra’) can be salvaged from permanent damage and the bourgeoning growth in computer aided diagnosis has led to major advances in stroke management. Abiding to the Preferred Reporting Items for Systematic Review and Meta–Analyses (PRISMA) guidelines, we have surveyed a total of 177 research papers published between 2010 and 2021 to highlight the current status and challenges faced by computer aided diagnosis (CAD), machine learning (ML) and deep learning (DL) based techniques for CT and MRI as prime modalities for stroke detection and lesion region segmentation. This work concludes by showcasing the current requirement of this domain, the preferred modality, and prospective research areas.
Special Issue “Wearable and BAN Sensors for Physical Rehabilitation and eHealth Architectures”
Authors: Domingues Maria de Fátima , Sciarrone Andrea , Radwan Ayman
Published: December 2021

The demographic shift of the population toward an increased number of elder citizens, together with the sedentary lifestyle we are adopting, is reflected in the increasingly debilitated physical health of the population [...]