bluuebunny/crossref_metadata_2025 huggingface dataset:
Revisiting Late Pleistocene Loess–Paleosol Sequences in the Azov Sea Region of Russia: Chronostratigraphy and Paleoenvironmental Record
Authors: Chen Jie , Stevens Thomas , Yang Taibao , Qiang Mingrui , Matishov Gennady , Konstantinov Evgeny , Kurbanov Redzhep , Zeng Biao , Shi Peihong
Published: February 2022

Loess-paleosol sequences are the most intensively studied terrestrial archives used for the reconstruction of Late Pleistocene environmental and climatic changes in the Azov Sea region, southwest Russia. Here we present a refined chronostratigraphy and a multiproxy record of Late Pleistocene environmental dynamics of the most complete and representative loess–paleosol sequences (Beglitsa and Chumbur-Kosa sections) from the Azov Sea region. We propose a new chronostratigraphy following the Chinese and Danubean loess stratigraphic models that refines the subdivision of the Last Interglacial paleosol (S1) complex in two Azov Sea sites, resolve the uncertainty of the stratigraphic position of the weakly developed paleosol (L1SSm) in Beglitsa section, and allow for direct correlation of the Azov Sea sections with those in the Danube Basin and the Chinese Loess Plateau. More importantly, it may serve as a basis for better constraining local and regional chronostratigraphic correlations, and facilitate the interpretation of climatic connections and possible forcing mechanisms responsible for the climatic trends in the region. In addition, a general succession of environmental dynamics is reconstructed from these two vital sections, which is broadly consistent with other loess records in the Dnieper Lowland and Lower Danube Basin, demonstrating similar climatic trends at Glacial–Interglacial time scales. Furthermore, our results have important implications for the chronostratigraphic representativeness of Beglitsa as a key regional loess section and for the reconstruction of the temporal and spatial evolution of Late Pleistocene climate in the Azov Sea region.
Study on Design Method of Pile Wall Combination Structure in a Deep Foundation Pit Considering Deformation Induced by Excavation
Authors: Wu Changjiang , Yu Jun , Cao Xiaojian , Shen Wuqin
Published: February 2022

The technology of pile wall combination in a deep foundation pit is a kind of technology to resist soil and water pressure by using the great stiffness of the retaining pile and basement wall. As a part of basement wall, the retaining pile can reduce the thickness of the basement wall. However, the influence of excavation deformation and the interaction mechanism between the retaining pile and basement wall are ignored in the current design theory of the pile wall combination in a deep foundation pit. Therefore, we proposed a composite structure model of an elastic subgrade beam associated with a continuous beam, and introduced an elastic spring to simulate the interaction between the pile and basement wall. The finite difference equation and calculation method were established to figure out the internal force and displacement of the pile wall combination structure, which was able to accurately calculate the internal force and deformation of the retaining pile during the excavation stage. Based on the above results, further investigation was carried on the interaction between the pile and basement wall during the construction and normal service stage of the basement structure. It was concluded that the basement wall could reduce the lateral displacement on the top of the retaining pile caused by removal of the strut and affect the bending moment of the upper part of the retaining pile among the depth of the basement wall. The bending moment of the basement wall was closely related to the deformation of the retaining pile and changes with the working condition. In addition, the results of factor sensitivity analysis showed that the pile-wall relative stiffness ratio, stiffness of the horizontal beam, and slab of the basement had a significant influence on the interaction between the pile and basement wall.
Physically Secure Wearable–Wearable Through-Body Interhuman Body Communication
Authors: Yang David , Maity Shovan , Sen Shreyas
Published: February 2022

Human body communication (HBC) has recently emerged as an alternative method to connect devices on and around the human body utilizing the electrical conductivity properties of the human body. HBC can be utilized to enable new interaction modalities between computing devices by enhancing the natural interaction of touch. It also provides the inherent benefit of security and energy-efficiency compared to a traditional wireless communication, such as Bluetooth, making it an attractive alternative. However, most state-of-the-art HBC demonstrations show communication between a wearable and an Earth ground–connected device, and there have been very few implementations of HBC systems demonstrating communication between two wearable devices. Also, most of the HBC implementations suffer from the problem of signal leakage out of the body which enables communication even without direct contact with the body. In this article, we present BodyWire which uses an electro-quasistatic HBC (EQS-HBC) technique to enable communication between two wearable devices and also confine the signal to a very close proximity to the body. We characterize the human body channel loss under different environment (office desk, laboratory, and outdoors), posture, and body location conditions to ascertain the effect of each of these on the overall channel loss. The measurement results show that the channel loss varies within a range of 15dB across all different posture, environmental conditions, and body location variation, illustrating the dynamic range of the signal available at the input of any receiver. Leakage measurements are also carried out from the devices to show the distance over which the signal is available away from the body to illustrate the security aspect of HBC and show its effect on the channel loss measurements. For the first time, a through-body interhuman channel loss characterization is presented. Finally, a demonstration of secure interhuman information exchange between two battery-operated wearable devices is shown through the BodyWire prototype, which shows the smallest form factor HBC demonstration according to the authors’ best knowledge.
Uric Acid Mediated the Association Between BMI and Postmenopausal Breast Cancer Incidence: A Bidirectional Mendelian Randomization Analysis and Prospective Cohort Study
Authors: Feng Yue , Fu Ming , Guan Xin , Wang Chenming , Yuan Fangfang , Bai Yansen , Meng Hua , Li Guyanan , Wei Wei , Li Hang , Li Mengying , Jie Jiali , Lu Yanjun , Guo Huan
Published: February 2022

Background

Observational epidemiological studies have reported the associations of high body mass index (BMI) with elevated serum uric acid (UA) level and increased risk of postmenopausal breast cancer. However, whether UA is causally induced by BMI and functioned in the BMI–breast cancer relationship remains unclear.

Methods

To elucidate the causality direction between BMI and serum UA, the bidirectional Mendelian randomization (MR) analyses were performed by using summarized data from the largest Asian genome-wide association studies (GWAS) of BMI and UA carried out in over 150,000 Japanese populations. Then, a total of 19,518 postmenopausal women from the Dongfeng–Tongji (DFTJ) cohort (with a mean 8.2-year follow-up) were included and analyzed on the associations of BMI and serum UA with incidence risk of postmenopausal breast cancer by using multivariable Cox proportional hazard regression models. Mediation analysis was further conducted among DFTJ cohort to assess the intermediate role of serum UA in the BMI–breast cancer association.

Results

In the bidirectional MR analyses, we observed that genetically determined BMI was causally associated with elevated serum UA [β(95% CI) = 0.225(0.111, 0.339), p < 0.001], but not vice versa. In the DFTJ cohort, each standard deviation (SD) increment in BMI (3.5 kg/m2) and UA (75.4 μmol/l) was associated with a separate 24% and 22% increased risk of postmenopausal breast cancer [HR(95% CI) = 1.24(1.07, 1.44) and 1.22(1.05, 1.42), respectively]. More importantly, serum UA could mediate 16.9% of the association between BMI and incident postmenopausal breast cancer.

Conclusions

The current findings revealed a causal effect of BMI on increasing serum UA and highlighted the mediating role of UA in the BMI–breast cancer relationship. Controlling the serum level of UA among overweight postmenopausal women may help to decrease their incident risk of breast cancer.
The Comparison of Fixed and Flexible Progestin Primed Ovarian Stimulation on Mature Oocyte Yield in Women at Risk of Premature Ovarian Insufficiency
Authors: Kalafat Erkan , Dizdar Merve , Turkgeldi Engin , Yildiz Sule , Keles Ipek , Ata Baris
Published: February 2022

While gonadotrophin releasing hormone (GnRH) antagonists have been the standard of pituitary suppression during ovarian stimulation for ART, progestin primed ovarian stimulation (PPOS) has emerged as an alternative. Progestins can be started simultaneously with gonadotrophins (fixed PPOS) or later in the cycle depending on follicle growth (flexible PPOS). However, the flexible and fixed PPOS regimens have not been directly compared as of yet. This was a retrospective cohort study including women with diminished ovarian reserve who underwent oocyte cryopreservation. All women underwent ovarian stimulation with a fixed 300 IU daily dose of FSH. The primary outcome was the number of MII oocyte retrieved per cycle. Secondary outcome measures included the incidence of premature LH surge (>10ng/mL) and number of follicles larger than 14mm on the day of maturation trigger. During the screening period 2 out of 97 cycles were cancelled before oocyte retrieval, one in each group yielding an overall cancelation rate of 2%. Among women who had oocyte retrieval, 65 underwent flexible and 30 fixed PPOS. At baseline women on fixed and flexible PPOS had similar age (mean difference: -2.17 years, 95% CI: -4.46 to 0.11) and serum AMH levels (mean difference: 0.10 ng/mL, 95% CI: -0.24 to 0.47). Slight imbalances between the groups were rectified with propensity score matching using age and AMH levels. The incidence of premature LH surge (RR: 1.47, 95% CI: 0.51 – 5.27, p = 0.50), follicle count larger than 14mm on hCG day (RR: 1.14, 95% CI: 0.93 – 1.42, p = 0.22), number of MII oocytes retrieved (RR: 0.95, 95% CI: 0.79 – 1.15, p = 0.61) were similar between flexible and fixed PPOS. The rate of no oocyte retrieval was same between the groups (0.0% both) but no formal estimation was possible. Flexible and fixed PPOS regimens had no appreciable differences regarding MII oocyte yield and the incidence of premature LH surges. Cycles without oocyte retrieval were rare in both groups and ultrasonographic parameters of gonadotropin response were similar. Our study suggests the performances of either progestin regimen are comparable in this group of women.
The Prevalence of Islet Autoantibodies in Children and Adolescents With Type 1 Diabetes Mellitus: A Global Scoping Review
Authors: Ross Carlo , Ward Zachary J. , Gomber Apoorva , Owais Maira , Yeh Jennifer M. , Reddy Ché-L. , Atun Rifat
Published: February 2022

Background and Purpose

Pancreatic islet autoantibodies (iAb) are the hallmark of autoimmunity in type 1 diabetes. A more comprehensive understanding of the global iAb prevalence could help reduce avertible morbidity and mortality among children and adolescents and contribute to the understanding in the observed differences in the incidence, prevalence and health outcomes of children and adolescents with type 1 diabetes across and within countries. We present the first scoping review that provides a global synthesis of the prevalence of iAb in children and adolescents with type 1 diabetes.

Research Design and Methods

We searched Ovid MEDLINE® with Daily Update, Embase (Elsevier, embase.com) and PubMed (National Library of Medicine -NCBI), for studies pertaining to prevalence in children and adolescents (0-19) with type 1 diabetes published between 1 Jan 1990 and 18 June 2021. Results were synthesized using Covidence systematic review software and meta-analysis was completed using R v3·6·1. Two reviewers independently screened abstracts with a third reviewer resolving conflicts (k= 0·92).

Results

The review revealed 125 studies from 48 different countries, with 92 from high-income countries. Globally, in new-onset type 1 diabetes, IA-2A was the most prevalent iAb 0·714 [95% CI (0·71, 0·72)], followed by ICA 0·681 [95% CI (0·67, 0·69)], ZnT8A was 0·654 [95% CI (0·64, 0·66)], GADA 0·636 [95% CI (0·63, 0·66)] and then IAA 0·424 [95% CI (0·42, 0·43)], with substantial variation across world regions. The weighted mean prevalence of IA-2A was more variable, highest in Europe at 0·749 [95% CI (0·74, 0·76)] followed by Northern America 0·662 [95% CI (0·64, 0·69)], Latin America and the Caribbean 0·632 [95% CI (0·54, 0·72)], Oceania 0·603 [95% CI (0·54, 0·67)], Asia 0·466 [95% CI (0·44, 0·50)] and Africa 0·311 [95% CI (0·23, 0·40)]. In established cases of type 1 diabetes, GADA was the most prevalent iAb 0·407 [95% CI (0·39, 0·42)] followed by ZnT8A 0·322 [95% CI (0·29, 0·36)], IA-2A 0·302 [95% CI (0·29, 0·32)], IAA 0·258 [95% CI (0·24, 0·26)] and ICA 0·145 [95% CI (0·13, 0·16)], again with substantial variation across world regions.

Conclusion

Understanding the global prevalence of iAb in children and adolescents with type 1 diabetes could help with earlier identification of those at-risk of developing type 1 diabetes and inform clinical practice, health policies, resource allocation, and targeted healthcare interventions to better screen, diagnose and manage children and adolescents with type 1 diabetes
The Effect of Aging on Quality of Life in Acromegaly Patients Under Treatment
Authors: Yamamoto Naoki , Urai Shin , Fukuoka Hidenori , Yamamoto Masaaki , Yoshida Kenichi , Suzuki Masaki , Shichi Hiroki , Fujita Yasunori , Kanie Keitaro , Iguchi Genzo , Takahashi Yutaka , Ogawa Wataru
Published: February 2022

Context

With the increasing number of older patients with acromegaly, it is important to understand the effects of aging on the quality of life (QoL) in acromegaly.

Objective

To investigate the factors associated with the QoL of older acromegaly patients.

Design

This was a single-center, retrospective, cross-sectional study conducted between 2014 and 2019.

Methods

Among 90 acromegaly patients at Kobe University Hospital, 74 who had completed the QoL evaluation under treatment were enrolled (age = 62.0 [50.7–70.0], female 52%). SF-36 and the AcroQoL questionnaire were used to quantify QoL. The patients were divided into two groups: the young and middle-aged group, aged <65 years (51.0 [46.0–59.2], n =42), and the older group, aged ≥65 years (70.5 [69.0–73.0], n =32). The factors associated with the QoL scores were analyzed using univariate and multivariate regression analyses.

Results

The scores for the physical component summary of SF-36 were negatively associated with age (P <0.01), while those for the mental or role/social component summary were positively associated (P <0.01, P =0.03, respectively). In contrast, AcroQoL scores were not associated with age. However, the different factors were associated with lower AcroQoL scores; arthropathy and higher BMI in the older group (P <0.01, and P =0.01, respectively), and treatment modalities and size of pituitary tumor in the young and middle-aged group (P <0.01, P =0.04, respectively). Replacement of hydrocortisone was commonly associated both in young and middle-aged group (P =0.04), and in older group (P =0.02).

Conclusion

We showed that the factors associated with impaired QoL differed in the young and middle-aged, and older patients with acromegaly. In older patients, arthropathy and higher BMI were associated with poor QoL. These suggest the importance of early diagnosis and appropriate treatment in preventing arthropathy in acromegaly.
Variation in Coarse Particulate Matter (PM10) and Its Characterization at Multiple Locations in the Semiarid Region
Authors: Zeb Bahadar , Alam Khan , Ditta Allah , Ullah Sami , Ali Hayssam M. , Ibrahim Muhammad , Salem Mohamed Z.M.
Published: February 2022

Introduction: The elemental composition and morphological study of particulate matter are very important to understand the nature of particles influencing the environment, climate, soil, and health.

Methods: The PM10 samples were collected during the winter season (2018) in Nowshera city, KPK, Pakistan, in three locations, namely, urban, industrial, and suburban. Scanning electron microscopy (SEM) and electron-dispersive X-ray (EDX) spectroscopy were used to examine the PM samples for morphological examination and elemental composition.

Results: The average mass concentrations of particulate matter (PM10) at the urban, industrial, and suburban locations were 238.5, 505.1, and 255.0 μg m−3, respectively. The average PM10 mass concentration was higher than the WHO and National Ambient Air Quality Standards (NAAQS). The results of EDX showed that samples contained variable amounts of thirteen elements, such as oxygen, carbon, silicon, magnesium, sodium, calcium, iron, aluminum, potassium, sulfur, titanium, gold, and chlorine. The probable sources of PM were biogenic like plant debris, pollen, and diatoms; geogenic like road dust and resuspended soil dust; and anthropogenic like carbonaceous particles and fly ash, as confirmed by SEM–EDX. The carbonaceous species, that is, OC and EC, had average values of 55.8 ± 13.1 and 4.6 ± 0.6, 5.2 ± 3.2, and 36.4 ± 10.4, 40.0 ± 2.6 and, 6.3 ± 0.2 in industrial, urban, and suburban locations, respectively. Similarly, OC/EC had average values of 12.0 ± 1.2, 8.0 ± 3.0, and 6.3 ± 0.2 in industrial, urban, and suburban locations, respectively. Highly significant correlations among water-soluble ions (K+), OC, and EC were found in each location.

Conclusions: The examined PM10 mass concentration in Nowshera city was above the thresholds of National Ambient Air Quality Standards (NAAQS) set by the U.S. Environmental Protection Agency (EPA). In addition, the concentration of pollutants was the highest at the industrial site compared to the other sites. The HYSPLIT model showed that the air mass originated from local sources like cement industries, brick kiln industries, and others.
Lack of Genetic Structure Among Populations of Striped Flea Beetle Phyllotreta striolata (Coleoptera: Chrysomelidae) Across Southern China
Authors: Li Qian , Li Guang-Mei , Zheng Yong-Li , Wei Shu-Jun
Published: February 2022

The striped flea beetle (SFB) Phyllotreta striolata (Fabricius) (Coleoptera: Chrysomelidae) is a major pest of cruciferous vegetables in southern China. The population diversity and genetic structure of SFB are unknown. Here, we assembled a draft genome for the SFB and characterized the distribution of microsatellites. Then, we developed 12 novel microsatellite markers across the genome. We used a segment of the cox1 gene and newly developed microsatellite markers to genotype the genetic diversity of SFB across southern China. There were 44 mitochondrial haplotypes in the SFB populations, with haplotype 2 as the most widespread. The population genetic differentiation was very low, indicated by FST-values (<0.05 except for Guangxi population with other populations based on cox1), high gene flow (4.10 and 44.88 of cox1 and microsatellite, respectively) and Principal Coordinate Analysis across all populations. Mantel test showed genetic distance in SFB was significantly associated with geographic distance based on microsatellites (R2 = 0.2373, P = 0.014) while result based on cox1 (R2 = 0.0365, P = 0.155) showed no significant difference. The phylogenetic analysis did not find any geographically related clades among all haplotypes. Analyses based on microsatellites showed a lack of population genetic structure among all populations. Our study provides a foundation for the future understanding of the ecology and evolution of SFB and its management.
DNA Methylation of ABC Transporters Differs in Native and Non-native Populations of Conyza canadensis L.
Authors: Shah Ambreen Tahir , Reshi Zafar A. , Altaf Mohammad
Published: February 2022

While differences in the methylation patterns of ABC transporters under different environmental conditions and their role in plant growth, development, and response to biotic and abiotic stresses are well documented, less is known about the variation in the methylation patterns of ABC transporters in plant species in the native and non-native ranges. In this study, we present the results of differences in methylation of ABC transporters of Conyza canadensis L. in its native (North America) and non-native (Kashmir Himalaya) ranges. Our data show that ABC transporter genes have reduced DNA methylation in Kashmir Himalaya than in North America. Furthermore, in the non-native range of Kashmir Himalaya, we found that ABC transporter genes have enriched RNA Pol-II binding and reduced nucleosome occupancy, both hallmarks of transcriptional activity. Taken together, our study showed differential DNA methylation in the ABC transporter genes in the native range of North America and non-native range of Kashmir Himalaya in Conyza canadensis and that the reduced DNA methylation and increased RNA Pol-II binding is one of the possible mechanisms through which this species in the non-native range of Kashmir Himalaya may show greater gene expression of ABC transporter genes. This increased ABC transporter gene expression may help the plant to grow in different environmental conditions in the non-native range. Furthermore, this study could pave way for more studies to better explain the enigmatic plant invasions of C. canadensis in the non-native range of Kashmir Himalaya.
Screening and Conjoint Analysis of Key lncRNAs for Milk Fat Metabolism in Dairy Cows
Authors: Mu Tong , Hu Honghong , Feng Xiaofang , Ma Yanfen , Wang Ying , Liu Jiamin , Yu Baojun , Wen Wan , Zhang Juan , Gu Yaling
Published: February 2022

Long noncoding RNAs (lncRNAs) play an important regulatory role in various biological processes as a key regulatory factor. However, the complete expression profile of lncRNAs in dairy cows and its function in milk fat synthesis are unknown. In this study, RNA sequencing (RNA-seq) was used to research the whole genome expression of lncRNAs and mRNA transcripts in high and low milk fat percentage (MFP) bovine mammary epithelial cells (BMECs), and joint analysis was carried out. We identified a total of 47 differentially expressed genes (DEGs) and 38 differentially expressed lncRNAs (DELs, Padj <0.05), enrichment analysis screened out 11 candidate DEGs that may regulate milk fat metabolism. Downregulated differential gene ENPP2 (The expression level in BMECs of high milk fat dairy cows was lower than that of low milk fat cows) and upregulated differential gene BCAT1 are more likely to participate in the milk fat metabolism, and its function needs further experiments verification. The enrichment analysis of target genes predicted by DELs identified 7 cis (co-localization) and 10 trans (co-expression) candidate target genes related to milk lipid metabolism, corresponding to a total of 18 DELs. Among them, the targeting relationship between long intervening/intergenic noncoding RNA (lincRNA) TCONS_00082721 and FABP4 is worthy of attention. One hundred and fifty-six competing endogenous RNAs (ceRNAs) interaction regulation networks related to milk fat metabolism were constructed based on the expression information of DELs, differential microRNAs (miRNAs), and lipid metabolism-related target genes. The regulatory network centered on miR-145 will be the focus of subsequent experimental research. The ceRNAs regulatory network related to TCONS_00082721 and TCONS_00172817 are more likely to be involved in milk fat synthesis. These results will provide new ways to understand the complex biology of dairy cow milk fat synthesis and provide valuable information for breed improvement of Chinese Holstein cow.
Antigen-Presenting Cells and T Cells Interact in a Specific Area of the Intestinal Mucosa Defined by the Ccl25-Ccr9 Axis in Medaka
Authors: Aghaallaei Narges , Agarwal Rashi , Benjaminsen Joergen , Lust Katharina , Bajoghli Baubak , Wittbrodt Joachim , Feijoo Carmen G.
Published: February 2022

Organized intestinal mucosal immune response appears to be restricted to tetrapods. In teleost fish, there is no evidence for the existence of a particular intestinal region that facilitates the interaction of antigen-presenting cells (APCs) and T cells, such as secondary lymphoid organs. Indeed, despite their importance in the defense against pathogens, the location and manner of APC-T cell interaction within the fish gut is unknown. Here, using non-invasive live imaging of newly developed transgenic reporter lines, we addressed the spatial organization and behavior of APCs and T cells in the intestine of medaka fish both during homeostasis and inflammation. We report that Ccr9a+ T cells are recruited to a band in the lamina propria next to the muscularis mucosa in which Ccl25-expressing cells are present. Ccr9a+ T cells contact APCs for several minutes, in a process mediated by connexin 43. This type of interaction was observed in homeostasis and inflammation, with the interaction being longer and more frequent during inflammation. Thus, our results demonstrate that the mucosal immune response in the intestine of medaka is organized and endowed with a specific region with specialized microenvironment and function.
Fishery Dynamics, Status, and Rebuilding Based on Catch-Only Data in Coastal Waters of China
Authors: Wang Linlong , Lin Li , Liu Yang , Zhai Lu , Ye Shen
Published: February 2022

China has become the largest contributor to marine fisheries in the world with its fishing fleets explosively increasing their fishing effort and resulting catch, but its fishery composition and sustainability have deteriorated. Limited information on fishery exploitation status encumbers effective resource management. In this study, a data-poor Monte Carlo method, the Catch-Maximum Sustainable Yield (CMSY) method, was used to estimate the historical exploited dynamics and current stock status of ten Chinese economic marine fish stocks, includingTrichiurus lepturus,Larimichthys crocea,Larimichthys polyactis,Thamnaconus modestus,Scomberomorus niphonius,Ilisha elongate,Decapterus maruadsi,Scomber japonicus,Engraulis japonicus, andClupea pallasii, which accounted for about 50% of total fish catches in the coastal waters of China and covered five functional groups (i.e., large, medium benthopelagic, large, medium, and small pelagic). SpeciesL. croceaandL. polyactishad been subjected to overfishing since the 1950s. The others showed a decreasing trend in biomass along with the explosively increasing fishing efforts since the 1990s. Benthopelagic fish experienced overfishing pressure about a decade earlier than pelagic species. All the fish stocks investigated in this study were depleted (current biomass lower than the biomass capable of producing maximum sustainable yields, i.e.,BFmsy). Also, a Schaefer model was used to assess stocks rebuilding status until 2030 under four exploitation scenarios, i.e., fishing mortality equals 0.5, 0.6, 0.8, or 0.95 timesFmsy. Most species stocks will likely recover to theBmsy, which indicates that reduction of fishing pressure is probably the most effective way for fishery recovery.
Improving Estuarine Hydrodynamic Forecasts Through Numerical Model Ensembles
Authors: Iglesias Isabel , Pinho José Luís , Avilez-Valente Paulo , Melo Willian , Bio Ana , Gomes Ana , Vieira José , Bastos Luísa , Veloso-Gomes Fernando
Published: February 2022

Numerical models are essential tools for the study and analysis of the hydrodynamics of estuarine systems. However, the model results contain uncertainties, which need to be minimized to increase the accuracy of predictions. In this work, the ensemble technique is proposed as a solution to improve hydrodynamic forecasts for estuarine regions. Two numerical models, openTELEMAC-MASCARET and Delft3D, were considered for the application of this technique to two Portuguese estuaries. Superensembles for three scenarios (summer, winter, and extreme event) were built to assess the effectiveness of the technique in improving water level prediction. Various weighing techniques were tested in the construction of the ensembles. Weighing techniques that consider the previous performance of each model alone outperformed other techniques. This was observed for all scenarios considered, at all sampling points and in both studied estuaries. The effect of the ensemble size was also analyzed. It was found that the size of the set is directly related to the prediction accuracy, with the best results provided by the superensembles with the highest number of elements. It is concluded that the combined use of several hydrodynamic models reduces the uncertainty of the results and increases the reliability and consistency of predictions for estuarine regions.
The Maximal Wrinkle Angle During the Bubble Collapse and Its Application to the Bubble Electrospinning
Authors: He Ji-Huan , Qian Man-Yu , Li Ya
Published: February 2022

Polymer bubbles are ubiquitously used for the fabrication of nanofibers by the bubble electrospinning. When a bubble is broken, the fragments tend to be wrinkled. The wrinkle angle plays an important in controlling the fiber morphology during the bubble electrospinning. This paper shows the maximal angle is about 49°, which is close to the experimental value of 50°. This maximal angle can be used for the optimal design of the nozzle in the bubble electrospinning for the fabrication of non-smooth nanofibers.
Bronchial Aspirate-Based Profiling Identifies MicroRNA Signatures Associated With COVID-19 and Fatal Disease in Critically Ill Patients
Authors: Molinero Marta , Benítez Iván D. , González Jessica , Gort-Paniello Clara , Moncusí-Moix Anna , Rodríguez-Jara Fátima , García-Hidalgo María C. , Torres Gerard , Vengoechea J. J. , Gómez Silvia , Cabo Ramón , Caballero Jesús , Bermejo-Martin Jesús F. , Ceccato Adrián , Fernández-Barat Laia , Ferrer Ricard , Garcia-Gasulla Dario , Menéndez Rosario , Motos Ana , Peñuelas Oscar , Riera Jordi , Torres Antoni , Barbé Ferran , de Gonzalo-Calvo David
Published: February 2022

Background

The pathophysiology of COVID-19-related critical illness is not completely understood. Here, we analyzed the microRNA (miRNA) profile of bronchial aspirate (BAS) samples from COVID-19 and non-COVID-19 patients admitted to the ICU to identify prognostic biomarkers of fatal outcomes and to define molecular pathways involved in the disease and adverse events.

Methods

Two patient populations were included (n = 89): (i) a study population composed of critically ill COVID-19 and non-COVID-19 patients; (ii) a prospective study cohort composed of COVID-19 survivors and non-survivors among patients assisted by invasive mechanical ventilation (IMV). BAS samples were obtained by bronchoaspiration during the ICU stay. The miRNA profile was analyzed using RT-qPCR. Detailed biomarker and bioinformatics analyses were performed.

Results

The deregulation in five miRNA ratios (miR-122-5p/miR-199a-5p, miR-125a-5p/miR-133a-3p, miR-155-5p/miR-486-5p, miR-214-3p/miR-222-3p, and miR-221-3p/miR-27a-3p) was observed when COVID-19 and non-COVID-19 patients were compared. In addition, five miRNA ratios segregated between ICU survivors and nonsurvivors (miR-1-3p/miR-124-3p, miR-125b-5p/miR-34a-5p, miR-126-3p/miR-16-5p, miR-199a-5p/miR-9-5p, and miR-221-3p/miR-491-5p). Through multivariable analysis, we constructed a miRNA ratio-based prediction model for ICU mortality that optimized the best combination of miRNA ratios (miR-125b-5p/miR-34a-5p, miR-199a-5p/miR-9-5p, and miR-221-3p/miR-491-5p). The model (AUC 0.85) and the miR-199a-5p/miR-9-5p ratio (AUC 0.80) showed an optimal discrimination value and outperformed the best clinical predictor for ICU mortality (days from first symptoms to IMV initiation, AUC 0.73). The survival analysis confirmed the usefulness of the miRNA ratio model and the individual ratio to identify patients at high risk of fatal outcomes following IMV initiation. Functional enrichment analyses identified pathological mechanisms implicated in fibrosis, coagulation, viral infections, immune responses and inflammation.

Conclusions

COVID-19 induces a specific miRNA signature in BAS from critically ill patients. In addition, specific miRNA ratios in BAS samples hold individual and collective potential to improve risk-based patient stratification following IMV initiation in COVID-19-related critical illness. The biological role of the host miRNA profiles may allow a better understanding of the different pathological axes of the disease.
A Novel Framework for Understanding the Pattern Identification of Traditional Asian Medicine From the Machine Learning Perspective
Authors: Bae Hyojin , Lee Sanghun , Lee Choong-yeol , Kim Chang-Eop
Published: February 2022

Pattern identification (PI), a unique diagnostic system of traditional Asian medicine, is the process of inferring the pathological nature or location of lesions based on observed symptoms. Despite its critical role in theory and practice, the information processing principles underlying PI systems are generally unclear. We present a novel framework for comprehending the PI system from a machine learning perspective. After a brief introduction to the dimensionality of the data, we propose that the PI system can be modeled as a dimensionality reduction process and discuss analytical issues that can be addressed using our framework. Our framework promotes a new approach in understanding the underlying mechanisms of the PI process with strong mathematical tools, thereby enriching the explanatory theories of traditional Asian medicine.
Prematurity and Low Birth Weight in Neonates as a Risk Factor for Obesity, Hypertension, and Chronic Kidney Disease in Pediatric and Adult Age
Authors: Grillo Maria Agostina , Mariani Gonzalo , Ferraris Jorge R.
Published: February 2022

Low weight at birth may be due to intrauterine growth restriction or premature birth. Preterm birth is more common in low- and middle-income countries: 60% of preterm birth occur in sub-Saharan African or South Asian countries. However, in some higher-income countries, preterm birth rates appear to be increasing in relation to a reduction in the lower threshold of fetal viability. The cutoff is at 22–23 weeks, with a birth weight of approximately 500 g, although in developed countries such as Japan, the viability cutoff described is 21–22 weeks. There is evidence of the long-term consequences of prenatal programming of organ function and its relationship among adult diseases, such as hypertension (HT), central obesity, diabetes, metabolic syndrome, and chronic kidney disease (CKD). Premature delivery before the completion of nephrogenesis and intrauterine growth restriction leads to a reduction in the number of nephrons that are larger due to compensatory hyperfiltration and hypertrophy, which predisposes to the development of CKD in adulthood. In these patients, the long-term strategies are early evaluation and therapeutic interventions to decrease the described complications, by screening for HT, microalbuminuria and proteinuria, ultrasound monitoring, and renal function, with the emphasis on preventive measures. This review describes the effects of fetal programming on renal development and the risk of obesity, HT, and CKD in the future in patients with low birth weight (LBW), and the follow-up and therapeutic interventions to reduce these complications.
The Value of Prenatal First Systolic Blood Pressure Can Predict Severe Preeclampsia and Birth Weight in Patients With Preeclampsia
Authors: Gan Bei , Wu Xiuyan , Lu Lin , Li Xuemei , Li Jianhua
Published: February 2022

Background

Preeclampsia is a serious complication of pregnancy that threatens the safety of the fetus and mother. We assessed the relationship between systolic blood pressure (SBP) in the early pregnancy stage (12 weeks) in patients with preeclampsia and the development of severe eclampsia and birth weight.

Methods

Patients were categorized based on the quartiles of the prenatal first SBP level. Logistic regression analysis was performed to assess whether prenatal first SBP was a risk factor for low birth weight and severe preeclampsia. The area under the receiver-operating characteristic curve (AUC) of sensitivity and specificity were used to predict the risk of low birth weight and severe preeclampsia.

Results

A total of 333 patients with preeclampsia were enrolled. There were 162 (48.6%) patients with severe preeclampsia and 270 (81.08%) cesareans. Group I patients with a prenatal first SBP ≤ 119 mmHg prenatal had a higher birth weight. Multiple logistic regression analysis showed that serum creatinine (p = 0.025), prenatal first SBP (p = 0.029), S-preeclampsia (p = 0.003), gestational age (p < 0.001), total cholesterol (TC) (p < 0.001), and low-density lipoprotein (LDL) (p < 0.001) were independent risk factors for low birth weight. Multiple logistic regression analysis showed that prenatal first SBP (p = 0.003), TC (p = 0.002), and B-type natriuretic peptide (BNP) (p < 0.001) were independent risk factors for severe preeclampsia. Compared with Group I (SBP ≤ 119 mmHg), the incidence of low birth weight for patients in groups III (131 ≤ SBP ≤ 138 mmHg) and IV (SBP ≥ 139 mmHg) was significantly higher. Even after correcting for age, gestational age, and biochemical indices, the difference remained statistically significant. The risk of diagnosed severe preeclampsia for patients in Groups IV (SBP ≥ 139 mmHg), III (131 ≤ SBP ≤ 138 mmHg), and II (120 ≤ SBP ≤ 130 mmHg) was significantly higher than that in Group I (SBP ≤ 119 mmHg). The AUC of the prenatal first SBP for predicting low birth weight and severe preeclampsia was 0.676 (95% CI 0.618–0.733, p < 0.001) and 0.727 (95% CI 0.673–0.781, p < 0.001), respectively, in patients with preeclampsia.

Conclusions

Prenatal first SBP was associated with birth weight and severe preeclampsia. Higher prenatal first SBP in patients with preeclampsia can predict low birth weight and severe preeclampsia.
HIF-1α Alleviates High-Glucose-Induced Renal Tubular Cell Injury by Promoting Parkin/PINK1-Mediated Mitophagy
Authors: Yu Lu , Wang Yulin , Guo Yan Hong , Wang Liuwei , Yang Zijun , Zhai Zi Han , Tang Lin
Published: February 2022

It is well-established that mitophagy leads to Diabetic Nephropathy (DN) and renal failure. Mitophagy mediated by a Hypoxia-inducible factor-1α (HIF-1α) plays a beneficial role in many diseases. Nevertheless, the mechanisms underlying HIF-1α-mediated mitophagy in DN remain unclear. This study defines the role of HIF-1α mediated mitophagy in DN. The expression of HIF-1α was upregulated in HK-2 cells in an High-Glucose (HG) environment, and the YC-1 (a specific inhibitor of HIF-1α) further exacerbated the hypoxia-induced mitochondrial dysfunction. Conversely, the HIF-1α-mediated protective effect was strengthened by scavenger N-acetylcysteine (NAC), a type of reactive oxygen species. Moreover, HIF-1α-Parkin/PINK1-mediated mitophagy prevented apoptosis and ROS production in HK-2 cells subjected to HG exposure. In summary, HIF-1α mediated mitophagy on HK-2 cells under HG conditions could alleviate DN, suggesting that it has huge prospects for DN treatment.
Advanced Therapy Medicinal Products' Translation in Europe: A Developers' Perspective
Authors: Pizevska Maja , Kaeda Jaspal , Fritsche Enrico , Elazaly Hisham , Reinke Petra , Amini Leila
Published: February 2022

Advanced Therapy Medicinal Products (ATMPs) comprising cell, gene, and tissue-engineered therapies have demonstrated enormous therapeutic benefits. However, their development is complex to be managed efficiently within currently existing regulatory frameworks. Legislation and regulation requirements for ATMPs must strike a balance between the patient safety while promoting innovations to optimize exploitation of these novel therapeutics. This paradox highlights the importance of on-going dynamic dialogue between all stakeholders and regulatory science to facilitate the development of pragmatic ATMP regulatory guidelines.
Online, Face-to-Face, or Blended Learning? Faculty and Medical Students' Perceptions During the COVID-19 Pandemic: A Mixed-Method Study
Authors: Atwa Hani , Shehata Mohamed Hany , Al-Ansari Ahmed , Kumar Archana , Jaradat Ahmed , Ahmed Jamil , Deifalla Abdelhalim
Published: February 2022

Background

COVID-19 pandemic forced educational institutions to adopt online methods which were inevitable to keep continuity of education across all academia after suspension of traditional educational systems. The aim of this study was to explore the experience of faculty and students of online and face-to-face learning, and their preference of the mode of learning after the pandemic.

Methods

This is a mixed-method study. Quantitative data was collected through a survey from 194 medical students and 33 faculty members, while qualitative data was collected through two focus group discussions with 9 students and another two with 13 faculty members. Quantitative variables were presented as means and standard deviations. Paired samples t-test and Chi-square test were used. Thematic analysis of qualitative data was used to code, interpret, and make sense of data.

Results

Mean scores of responses of faculty members and students were higher for face-to-face and blended learning compared to online learning in all survey statements with statistically significant differences. More than half of the students (53.1%) preferred the face-to-face mode of learning, while most of the faculty members (60.6%) preferred the blended mode of learning. Qualitative analysis identified five themes, namely: “Transforming the way theoretical teaching sessions are given,” “Face-to-face teaching at campus cannot be replaced for some types of education,” “Interaction in online sessions is limited,” “Problems and challenges of online examinations,” and “Technical issues and challenges of online education.” It revealed suggestions that at least 30% of the curriculum could be taught online post-COVID-19. Some aspects of clinically oriented teaching including history taking and case discussions can also be delivered online in the future. Faculty members and students reported that dealing with online education was not difficult, although the transition was not smooth.

Conclusion

Medical students and faculty members were in favor of face-to-face and blended modes of learning. However, they perceived online mode of learning as an acceptable adaptation in theoretical teaching and in some clinically oriented teaching including history taking and clinical case discussions. Although face-to-face education in medicine is irreplaceable, the blended mode of learning remains an acceptable and practical solution for the post-COVID era.
Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials
Authors: Khalil Maha A. , El-Shanshoury Abd El-Raheem R. , Alghamdi Maha A. , Alsalmi Fatin A. , Mohamed Samia F. , Sun Jianzhong , Ali Sameh S.
Published: February 2022

Nanoparticles have recently emerged as a popular research topic. Because of their potential applications in therapeutic applications, biosynthesized silver nanoparticles (Bio-AgNPs) have gained much attention in recent years. Cell-free extracts (CFE) from a marine culture of actinobacteria and silver nitrate were used to reduce Ag+ ions and create Bio-AgNPs. Nocardiopsis dasonvillei KY772427, a new silver-tolerant actinomycete strain, was isolated from marine water and used to synthesize AgNPs. In order to characterize Bio-AgNPs, UV-Vis spectral analysis, Fourier transform infrared (FTIR), transmission electron microscopy (TEM), and dynamic light scattering spectroscopy (DLS) were all utilized. Using UV–Vis spectroscopy, a peak in the surface plasmon resonance (SPR) spectrum at 430 nm revealed the presence of Bio-AgNPs. The TEM revealed spherical AgNPs with a diameter of 29.28 nm. DLS determined that Bio-AgNPs have a diameter of 56.1 nm and a negative surface charge (−1.46 mV). The minimum inhibitory concentration (MIC) of Bio-AgNPs was determined against microbial strains. Using resazurin-based microtiter dilution, the synergistic effect of Bio-AgNPs with antimicrobials was investigated. Pseudomonas aeruginosa had the lowest MIC of Bio-AgNPs (4 μg/ml). Surprisingly, the combination of antimicrobials and Bio-AgNPs had a significant synergistic effect on the tested strains. The insecticidal activity of Bio-AgNPs (200 μg/ml) against Macrosiphum rosae was found to be maximal after 36 h. Additionally, Bio-AgNPs demonstrated significant scavenging activity against 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH–) radicals, with IC50 values of 4.08 and 8.9 g/ml, respectively. In vitro studies using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay revealed a concentration-dependent decrease in cell viability when CaCo2 cells were exposed to Bio-AgNPs. With the decrease in cell viability, lactate dehydrogenase leakage (LDH) increased. The findings of this study open up a new avenue for the use of marine Nocardiopsis dasonvillei to produce Bio-AgNPs, which have significant antimicrobial, antioxidant, insecticidal, and anticancer potential.
Succession Pattern in Soil Micro-Ecology Under Tobacco (Nicotiana tabacum L.) Continuous Cropping Circumstances in Yunnan Province of Southwest China
Authors: Chen Dan , Zhou Yujie , Wang Mei , Mujtaba Munir Mehr Ahmed , Lian Jiapan , Yu Song , Dai Kuai , Yang Xiaoe
Published: February 2022

Continuous cropping obstacle (CCO) is a common phenomenon in agricultural production and extremely threatens the sustainable development of agriculture. To clarify the potential keystone factors causing tobacco (Nicotiana tabacum L.) CCO, tobacco plants, topsoil, and rhizosphere soil were sampled from the fields with no, slight, and severe tobacco disease in Dali and Yuxi of Yunnan province in China. The physicochemical properties of topsoil and rhizosphere soil, the phenolic acids (PAs) contents in rhizosphere soil, and elemental contents in topsoil, rhizosphere soil, and tobacco plants were analyzed. Microbial diversity in rhizosphere soil was determined by the metagenomic sequencing method. The results showed that soil pH, texture, cation exchange capacity, organic matter, TC, TN, and available K contents showed a significant difference (p < 0.05) in soil physicochemical properties. There was a deficiency of B, K, Mg, and Mn contents in soil and/or tobacco plants. The contents of PAs, especially syringic acid in rhizosphere soil, varied significantly among the three sampling groups (p < 0.05). Meanwhile, microbial communities and functional genes changed from beneficial to harmful, showing an intimate correlation with soil pH and syringic acid content. It can be concluded that tobacco CCO could be allocated to the imbalance of soil micro-ecology, which possessed a regional feature at the two sampling sites.