bluuebunny/crossref_metadata_2025 huggingface dataset:
Transport Model Approach to Λ and Λ¯ Polarization in Heavy-Ion Collisions
Authors: Bravina Larissa , Bugaev Kyrill , Vitiuk Oleksandr , Zabrodin Evgeny
Published: October 2021

This paper investigates the symmetry breaking between the polarizations of Λ and Λ¯ hyperons in relativistic collisions of heavy ions at intermediate and low energies. The microscopic transport model UrQMD is employed to study the thermal vorticity of hot and dense nuclear matter formed in non-central Au + Au collisions at center-of-mass energies 7.7≤sNN≤62.4 GeV. The whole volume of an expanding fireball is subdivided into small cubic cells. Then, we trace the final Λ and Λ¯ hyperons back to their last interaction point within a certain cell. Extracting the bulk parameters, such as energy density, net baryon density, and net strangeness of the hot and dense medium in the cell, one can obtain the cell temperature and the chemical potentials at the time of the hyperon emission. To do this, the extracted characteristics have to be fitted to the statistical model (SM) of ideal hadron gas. After that, the vorticity of nuclear matter and polarization of both hyperons are calculated. We found that the polarization of both Λ and Λ¯ increases with decreasing energy of heavy-ion collisions. The stronger polarization of Λ¯ is explained by (i) the slightly different freeze-out conditions of both hyperons and (ii) the different space–time distributions of Λ and Λ¯.
A Physically Meaningful Relativistic Description of the Spin State of an Electron
Authors: Friedman Yaakov
Published: October 2021

We introduced a new model to present the states of a two-state quantum system. The space is the complexified Minkowski space. The Lorentz group acts by the linear extension of its action on the four-vectors. We applied this model to represent the spin state of an electron or any relativistic spin 1/2 particle. The spin state of such particle is of the form U+iS, where U is the four-velocity of the particle in the lab frame, and S is the 4D spin in this frame. Under this description, the transition probability between two pure spin states ϱ1 and ϱ2 of particles moving with the same velocity are defined by use of Minkowski dot product as 12<ϱ2|ϱ1>. This transition probability is Lorentz invariant, coincide with the quantum mechanics prediction and thus agree with the experimental results testing quantum mechanics predictions based on Bell’s inequality. For a a particle of mass m and charge q with the spin state ϱ, the total momentum is mcϱ and the electromagnetic momentum is qϱ. This imply that the Landé g factor for such particles must be g=2. We obtain an evolution equation of the spin state in an electromagnetic field which defines correctly the anomalous Zeeman effect and the fine structure splitting.
Effect of Asymmetry of Channels on Flows in Parallel Plates with a Sudden Expansion
Authors: Masuda Takuya , Tagawa Toshio
Published: October 2021

In order to quantitatively grasp the influence of asymmetry of a channel, flow in an eccentric sudden expansion channel, in which the channel centers are different on the upstream and downstream sides, was calculated to be less than the Reynolds number of 400, where the expansion rate was 2. The asymmetry of a channel is expressed by an eccentricity S, where a symmetric expansion channel is S = 0 and a channel with one side step is S = 1. Both flows firstly reattached on the wall located on the short and long side of a sudden expansion and were observed in the range of S ≤ 0.2, although only the former was seen in the range of S > 0.2. The critical Reynolds number of the multiple solutions increases parabolically to S. At least two separation vortices occur, and the third separation vortex is generated in both solutions above the critical Reynolds number of the third vortex. The length of an entrance region increases linearly to the Reynolds number and slightly with the increase in S. The pressure drop coefficient is proportional to the power of the Reynolds number and increases with S.
On 3-Rainbow Domination Number of Generalized Petersen Graphs P(6k,k)
Authors: Erveš Rija , Žerovnik Janez
Published: October 2021

We obtain new results on 3-rainbow domination numbers of generalized Petersen graphs P(6k,k). In some cases, for some infinite families, exact values are established; in all other cases, the lower and upper bounds with small gaps are given. We also define singleton rainbow domination, where the sets assigned have a cardinality of, at most, one, and provide analogous results for this special case of rainbow domination.
Time-Dependent Conformal Transformations and the Propagator for Quadratic Systems
Authors: Zhao Qiliang , Zhang Pengming , Horvathy Peter A.
Published: October 2021

The method proposed by Inomata and his collaborators allows us to transform a damped Caldirola–Kanai oscillator with a time-dependent frequency to one with a constant frequency and no friction by redefining the time variable, obtained by solving an Ermakov–Milne–Pinney equation. Their mapping “Eisenhart–Duval” lifts as a conformal transformation between two appropriate Bargmann spaces. The quantum propagator is calculated also by bringing the quadratic system to free form by another time-dependent Bargmann-conformal transformation, which generalizes the one introduced before by Niederer and is related to the mapping proposed by Arnold. Our approach allows us to extend the Maslov phase correction to an arbitrary time-dependent frequency. The method is illustrated by the Mathieu profile.
Similarity Analysis of Methods for Objective Determination of Weights in Multi-Criteria Decision Support Systems
Authors: Paradowski Bartosz , Shekhovtsov Andrii , Bączkiewicz Aleksandra , Kizielewicz Bartłomiej , Sałabun Wojciech
Published: October 2021

Decision support systems (DSS) are currently developing rapidly and are increasingly used in various fields. More often, those systems are inseparable from information-based systems and computer systems. Therefore, from a methodical point of view, the algorithms implemented in the DSS play a critical role. In this aspect, multi-criteria decision support (MCDA) methods are widely used. As research progresses, many MCDA methods and algorithms for the objective identification of the significance of individual criteria of the MCDA models were developed. In this paper, an analysis of available objective methods for criteria weighting is presented. Additionally, the authors presented the implementation of the system that provides easy and accessible weight calculations for any decision matrix with the possibility of comparing results of different weighting methods. The results of weighting methods were compared using carefully selected similarity coefficients to emphasise the correlation of the resulting weights. The performed research shows that every method should provide distinctive weights considering input data, emphasising the importance of choosing the correct method for a given multi-criteria decision support model and DSS.
Generalized Higher Order Preinvex Functions and Equilibrium-like Problems
Authors: Ma Jiangming , Noor Muhammad Aslam , Noor Khalida Inayat
Published: October 2021

Equilibrium problems and variational inequalities are connected to the symmetry concepts, which play important roles in many fields of sciences. Some new preinvex functions, which are called generalized preinvex functions, with the bifunction ζ(.,.) and an arbitrary function k, are introduced and studied. Under the normed spaces, new parallelograms laws are taken as an application of the generalized preinvex functions. The equilibrium-like problems are represented as the minimum values of generalized preinvex functions under the kζ-invex sets. Some new inertial methods are proposed and researched to solve the higher order directional equilibrium-like problem, Convergence criteria of the our methods is discussed, along with some unresolved issues.
Estimation and Hypothesis Testing for the Parameters of Multivariate Zero Inflated Generalized Poisson Regression Model
Authors: Sari Dewi Novita , Purhadi Purhadi , Rahayu Santi Puteri , Irhamah Irhamah
Published: October 2021

We propose a multivariate regression model called Multivariate Zero Inflated Generalized Poisson Regression (MZIGPR) type II. This model further develops the Bivariate Zero Inflated Generalized Poisson Regression (BZIGPR) type II. This study aims to develop parameter estimation, test statistics, and hypothesis testing, both simultaneously and partially, for significant parameters of the MZIGPR model. The steps of the EM algorithm for obtaining the parameter estimator are also described in this article. We use Berndt–Hall–Hall–Hausman (BHHH) numerical iteration to optimize the EM algorithm. Simultaneous testing is carried out using the maximum likelihood ratio test (MLRT) and the Wald test to partially assess the hypothesis. The proposed MZIGPR model is then used to model the three response variables: the number of maternal childbirth deaths, the number of postpartum maternal deaths, and the number of stillbirths with four predictors. The units of observation are the sub-districts of the Pekalongan Residency, Indonesia. The indicate overdispersion in the data on the number of maternal childbirth deaths and stillbirths, and underdispersion in the data on the number of postpartum maternal deaths. The empirical studies show that the three response variables are significantly affected by all the predictor variables.
Possible Role of Non-Stationarity of Magnetohydrodynamic Turbulence in Understanding of Geomagnetic Excursions
Authors: Mizerski Krzysztof Andrzej
Published: October 2021

The natural simplifying assumptions often put forward in the theoretical investigations of the magnetohydrodynamic turbulence are that the turbulent flow is statistically isotropic, homogeneous and stationary. Of course, the natural turbulence in the planetary interiors, such as the liquid core of the Earth is neither, which has important consequences for the dynamics of the planetary magnetic fields generated via the hydromagnetic dynamo mechanism operating in the interiors of the planets. Here we concentrate on the relaxation of the assumption of statistical stationarity of the turbulent flow and study the effect of turbulent wave fields in the Earth’s core, which induces non-stationarity, on the turbulent resistivity in the non-reflectionally symmetric flow and the geodynamo effect. It is shown that the electromotive force, including the so-called α-effect and the turbulent magnetic diffusivity η¯, induced by non-stationary turbulence, evolves slowly in time. However, the turbulent α¯ coefficient, responsible for the dynamo action and η¯ evolve differently in time, thus creating periods of enhanced and suppressed turbulent diffusion and dynamo action somewhat independently. In particular, periods of enhanced α¯ may coincide with periods of suppressed diffusion, leading to a stable and strong field period. On the other hand, it is shown that when enhanced diffusion occurs simultaneously with suppression of the α-effect, this leads to a sharp drop in the intensity of the large-scale field, corresponding to a geomagnetic excursion.
Spin Experimentation with Unpolarized Colliding Beams at the LHC
Authors: Troshin Sergey
Published: October 2021

A brief recollection of the problems related to a significant hyperon polarization observed in pp-collisions is given with an emphasis on the general role of spin in the dynamics of hadron interactions. The old, unsolved problem of the observation of a significant hyperon polarization can provide new insights as a result of the measurements of energies at the LHC; in combination with other measurements, these can be used to tag the QGP formation in pp-collisions with colliding beams. Polarization studies in the processes of hyperon production do not require the use of polarized beams or targets and can be performed in the existing experimental environment at the LHC. Model predictions based on the chiral dynamics and pictures of the impact parameter are presented for the illustration of a possible dynamical mechanism that leads to a hyperon polarization.
Slow Invariant Manifold of Laser with Feedback
Authors: Ginoux Jean-Marc , Meucci Riccardo
Published: October 2021

Previous studies have demonstrated, experimentally and theoretically, the existence of slow–fast evolutions, i.e., slow chaotic spiking sequences in the dynamics of a semiconductor laser with AC-coupled optoelectronic feedback. In this work, the so-called Flow Curvature Method was used, which provides the slow invariant manifold analytical equation of such a laser model and also highlights its symmetries if any exist. This equation and its graphical representation in the phase space enable, on the one hand, discriminating the slow evolution of the trajectory curves from the fast one and, on the other hand, improving our understanding of this slow–fast regime.
Covalently Bonded Fullerene Nano-Aggregates (C60)n: Digitalizing Their Energy–Topology–Symmetry
Authors: Sabirov Denis Sh. , Ori Ottorino , Tukhbatullina Alina A. , Shepelevich Igor S.
Published: October 2021

Fullerene dimers and oligomers are attractive molecular objects with an intermediate position between the molecules and nanostructures. Due to the size, computationally assessing their structures and molecular properties is challenging, as it currently requires high-cost quantum chemical techniques. In this work, we have jointly studied energies, topological (Wiener indices and roundness), and information theoretic (information entropy) descriptors, and have obtained regularities in triad ‘energy–topology–symmetry’. We have found that the topological indices are convenient to indicating the most and least reactive atoms of the fullerene dimer structures, whereas information entropy is more suitable to evaluate odd–even effects on the symmetry of (C60)n. Quantum chemically assessed stabilities of selected C120 structures, as well as linear and zigzag (C60)n, are discussed.
Sharp Upper and Lower Bounds of VDB Topological Indices of Digraphs
Authors: Monsalve Juan , Rada Juan
Published: October 2021

A vertex-degree-based (VDB, for short) topological index φ induced by the numbers φij was recently defined for a digraph D, as φD=12∑uvφdu+dv−, where du+ denotes the out-degree of the vertex u,dv− denotes the in-degree of the vertex v, and the sum runs over the set of arcs uv of D. This definition generalizes the concept of a VDB topological index of a graph. In a general setting, we find sharp lower and upper bounds of a symmetric VDB topological index over Dn, the set of all digraphs with n non-isolated vertices. Applications to well-known topological indices are deduced. We also determine extremal values of symmetric VDB topological indices over OTn and OG, the set of oriented trees with n vertices, and the set of all orientations of a fixed graph G, respectively.
Global Symmetries, Local Symmetries and Groupoids
Authors: Petitjean Michel
Published: October 2021

Local symmetries are primarily defined in the case of spacetime, but several authors have defined them outside this context, sometimes with the help of groupoids. We show that, in many cases, local symmetries can be defined as global symmetries. We also show that groups can be used, rather than groupoids, to handle local symmetries. Examples are given for graphs and networks, color symmetry and tilings. The definition of local symmetry in physics is also discussed.
Low-Dose Neubotulinum Toxin A versus Low-Dose Abobotulinum Toxin A Injection for the Treatment of Cervical Dystonia: A Multicenter, 48-Week, Prospective, Double-Blinded, Randomized Crossover Design Study
Authors: Kongsaengdao Subsai , Arayawithchanont Arkhom , Samintharapanya Kanoksri , Rojanapitayakorn Pichai , Maneeton Benchalak , Maneeton Narong
Published: October 2021

Various types of botulinum toxin (BoNT) have been studied to treat cervical dystonia (CD). Although high-dose BoNT has proven efficacy, it increases the risk of adverse events. For this reason, this study was planned to identify the non-inferiority efficacy, tolerability, and safety of low-dose neubotulinum toxin A (Neu-BoNT-A) versus low-dose abobotulinum toxin A (Abo-BoNT-A) in CD treatment. The 48-week, prospective, randomized, controlled crossover design study of CD treatment, with 50-unit Neu-BoNT-A and 250-unit Abo-BoNT-A injections at 12-week intervals, was conducted over a 24-week treatment period. This study used the following standardized rating scales to assess the efficacy of BoNT: the Toronto Western Spasmodic Torticollis Rating Scale (TWSTRS); health-related quality of life (HRQoL); the Cervical Dystonia Impact Profile (CDIP-58); the Short Form 36 health survey questionnaire (SF-36); and, for the depressive symptoms of CD patients, the Center for Epidemiological Studies-Depression Scale (CES-D) and the Patient Health Questionnaire-9 (PHQ-9). Fifty-two CD patients were enrolled from October 2019 to January 2021. The mean scores of the TWSTRS total at the post-treatments in both Neu-BoNT-A and Abo-BoNT-A had a significant reduction from baseline (p = 0.008 and 0.002, respectively). However, the mean changes of the TWSTRS total at the 12- and 24-week treatments between the two treatment groups were not significantly different (p = 0.284 and 0.129, respectively). The mean scores of the HRQoL questionnaires (the CIDP-58 and the SF-36) and the depressive symptoms (the CES-D and the PHQ-9) in both treated groups at the post-treatments did not significantly decrease from baseline and were comparable. Two patients treated with Abo-BoNT-A (250 units) reported cervical tension and benign paroxysmal positional vertigo (BPPV). There were no serious adverse events reported. Though both low-dose BoNT-As were effective at improving clinical symptoms without significant side effects, both treatments did not predict change in quality of life and depression. With the non-inferiority criteria, low-dose Neu-BoNT-A has a similar efficacy, safety, and tolerability to Abo-BoNT-A.
Overall Exposure of European Adult Population to Mycotoxins by Statistically Modelled Biomonitoring Data
Authors: De Santis Barbara , Debegnach Francesca , Toscano Piero , Crisci Alfonso , Battilani Paola , Brera Carlo
Published: October 2021

This study presents the exposure scenario to mycotoxins of adult population throughout Europe. The urinary biomarkers values were obtained by modelling data from two European projects. Exposure to AFB1, OTA, CIT, FBs, DON, NIV and T2/HT2 are presented. The main output obtained refers to a concern for public health about AFM1, FBs, T2/HT2 and NIV, and low concern for OTA, DON and CIT. The margin of exposure for AFM1 did not respect the reference value of 10,000 considered of low priority for risk; for Fusarium toxins, FBs and T2/HT2, probable daily intake (PDI) values resulted about ten times higher than their tolerable daily intake and NIV presented the most critical situation with a calculated PDI 30 times higher than the reference TDI value. North and South Europe scenarios were also depicted by clustering biomonitoring data. OTA and DON showed to be prevalent in Northern countries and the opposite was noticed for ZEN, higher in Southern countries. The critical issues of the availability of records feeding the dataset and of the accuracy of excretion rate for some mycotoxins are source of uncertainty for the reliability of the outputs, nevertheless the time is ripe for asking for more concrete HBM values and/or HBM-HBGV which would help in interpreting the burden of mycotoxins in Europe.
New Insights into the Potential Cytotoxic Role of Bacillus cytotoxicus Cytotoxin K-1
Authors: Koné Klèma Marcel , Hinnekens Pauline , Jovanovic Jelena , Rajkovic Andreja , Mahillon Jacques
Published: October 2021

The thermotolerant representative of the Bacillus cereus group, Bacillus cytotoxicus, reliably harbors the coding gene of cytotoxin K-1 (CytK-1). This protein is a highly cytotoxic variant of CytK toxin, initially recovered from a diarrheal foodborne outbreak that caused the death of three people. In recent years, the cytotoxicity of B. cytotoxicus has become controversial, with some strains displaying a high cytotoxicity while others show no cytotoxicity towards cell lines. In order to better circumscribe the potential pathogenic role of CytK-1, knockout (KO) mutants were constructed in two B. cytotoxicus strains, E8.1 and E28.3. The complementation of the cytK-1 KO mutation was implemented in a mutant strain lacking in the cytK-1 gene. Using the tetrazolium salt (MTT) method, cytotoxicity tests of the cytK-1 KO and complemented mutants, as well as those of their wild-type strains, were carried out on Caco-2 cells. The results showed that cytK-1 KO mutants were significantly less cytotoxic than the parental wild-type strains. However, the complemented mutant was as cytotoxic as the wild-type, suggesting that CytK-1 is the major cytotoxicity factor in B. cytotoxicus.
Effects of Uremic Clearance Granules in Uremic Pruritus: A Meta-Analysis
Authors: Lu Ping-Hsun , Wang Jen-Yu , Chuo Hui-En , Lu Po-Hsuan
Published: October 2021

Uremic pruritus is common among patients with advanced or end-stage renal disease, with an incidence of >40% among patients on dialysis. Uremic clearance granules (UCGs) are effective in managing uremic pruritus and delay the progression of chronic kidney disease. We conducted a systematic review and a meta-analysis to evaluate the efficacy of UCG in patients with uremic pruritus. Several electronic databases were searched systematically from their inceptions until 19 July 2021. Randomized control trials evaluating the efficacy of UCG in patients with uremic pruritus were selected. Eleven trials including 894 participants were published between 2011 and 2021. Patients administered UCGs had a significantly decreased visual analog scale score (mean difference [MD], −2.02; 95% confidence interval [CI], −2.17 to −1.88), serum levels of hsCRP (MD, −2.07 mg/dL; 95% CI, −2.89 to −1.25; p < 0.00001), TNF-α (MD, −15.23 mg/L; 95% CI, −20.00 to −10.47; p < 0.00001]), β2-MG (MD, −10.18 mg/L; 95% CI, −15.43 to −4.93; p < 0.00001), and IL-6 (MD, −6.13 mg/L; 95% CI, −7.42 to −4.84; p < 0.00001). In addition, UCGs significantly reduced serum levels of creatinine, BUN, PTH, iPTH, phosphorus, and the overall effectiveness rate. UCGs could be an attractive complementary therapy for patients with uremic pruritus.
Penicillium expansum Impact and Patulin Accumulation on Conventional and Traditional Apple Cultivars
Authors: Lončarić Ante , Šarkanj Bojan , Gotal Ana-Marija , Kovač Marija , Nevistić Ante , Fruk Goran , Skendrović Babojelić Martina , Babić Jurislav , Miličević Borislav , Kovač Tihomir
Published: October 2021

Penicillium expansum is a necrotrophic plant pathogen among the most ubiquitous fungi disseminated worldwide. It causes blue mould rot in apples during storage, transport and sale, threatening human health by secreting patulin, a toxic secondary metabolite that contaminates apples and apple-derived products. Nevertheless, there is still a lack of sufficient data regarding the resistance of different apple cultivars to P. expansum, especially ancient ones, which showed to possess certain resistance to plant diseases. In this work, we investigated the polyphenol profile of 12 traditional and 8 conventional apple cultivar and their resistance to P. expansum CBS 325.48. Eight polyphenolic compounds were detected; the most prominent were catechin, epicatechin and gallic acid. The highest content of catechin was detected in ‘Apistar’—91.26 mg/100 g of fresh weight (FW), epicatechin in ‘Bobovac’—67.00 mg/100 g of FW, and gallic acid in ‘Bobovac’ and ‘Kraljevčica’—8.35 and 7.40 mg/100 g of FW, respectively. The highest content of patulin was detected in ‘Kraljevčica’ followed by ‘Apistar’—1687 and 1435 µg/kg, respectively. In apple cultivars ‘Brčko’, ‘Adamčica’ and ‘Idared’, patulin was not detected. Furthermore, the patulin content was positively correlated with gallic acid (r = 0.4226; p = 0.002), catechin (r = 0.3717; p = 0.008) and epicatechin (r = 0.3305; p = 0.019). This fact indicates that higher contents of gallic acid, catechin and epicatechin negatively affected and boost patulin concentration in examined apple cultivars. This can be related to the prooxidant activity of polyphenolic compounds and sensitivity of P. expansum to the disturbance of oxidative status.
Toward Revealing Microcystin Distribution in Mouse Liver Tissue Using MALDI-MS Imaging
Authors: Kucheriavaia Daria , Veličković Dušan , Peraino Nicholas , Lad Apurva , Kennedy David J. , Haller Steven T. , Westrick Judy A. , Isailovic Dragan
Published: October 2021

Cyanotoxins can be found in water and air during cyanobacterial harmful algal blooms (cHABs) in lakes and rivers. Therefore, it is very important to monitor their potential uptake by animals and humans as well as their health effects and distribution in affected organs. Herein, the distribution of hepatotoxic peptide microcystin-LR (MC-LR) is investigated in liver tissues of mice gavaged with this most common MC congener. Preliminary matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging experiments performed using a non-automated MALDI matrix deposition device and a MALDI-time-of-flight (TOF) mass spectrometer yielded ambiguous results in terms of MC-LR distribution in liver samples obtained from MC-LR-gavaged mice. The tissue preparation for MALDI-MS imaging was improved by using an automated sprayer for matrix deposition, and liver sections were imaged using an Nd:YAG MALDI laser coupled to a 15 Tesla Fourier-transform ion cyclotron resonance (FT-ICR)-mass spectrometer. MALDI-FT-ICR-MS imaging provided unambiguous detection of protonated MC-LR (calculated m/z 995.5560, z = +1) and the sodium adduct of MC-LR (m/z 1017.5380, z = +1) in liver sections from gavaged mice with great mass accuracy and ultra-high mass resolution. Since both covalently bound and free MC-LR can be found in liver of mice exposed to this toxin, the present results indicate that the distribution of free microcystins in tissue sections from affected organs, such as liver, can be monitored with high-resolution MALDI-MS imaging.
Immunotoxic Effects Induced by Microcystins and Cylindrospermopsin: A Review
Authors: Diez-Quijada Leticia , Benítez-González Maria del Monte , Puerto María , Jos Angeles , Cameán Ana M.
Published: October 2021

Cyanotoxin occurrence is gaining importance due to anthropogenic activities, climate change and eutrophication. Among them, Microcystins (MCs) and Cylindrospermopsin (CYN) are the most frequently studied due to their ubiquity and toxicity. Although MCs are primary classified as hepatotoxins and CYN as a cytotoxin, they have been shown to induce deleterious effects in a wide range of organs. However, their effects on the immune system are as yet scarcely investigated. Thus, to know the impact of cyanotoxins on the immune system, due to its importance in organisms’ homeostasis, is considered of interest. A review of the scientific literature dealing with the immunotoxicity of MCs and CYN has been performed, and both in vitro and in vivo studies have been considered. Results have confirmed the scarcity of reports on the topic, particularly for CYN. Decreased cell viability, apoptosis or altered functions of immune cells, and changed levels and mRNA expression of cytokines are among the most common effects reported. Underlying mechanisms, however, are still not yet fully elucidated. Further research is needed in order to have a full picture of cyanotoxin immunotoxicity.
Assessment of Citrinin in Spices and Infant Cereals Using Immunoaffinity Column Clean-Up with HPLC-Fluorescence Detection
Authors: Mair Christopher , Norris Michael , Donnelly Carol , Leeman Dave , Brown Phyllis , Marley Elaine , Milligan Claire , Mackay Naomi
Published: October 2021

Historically, the analysis of citrinin has mainly been performed on cereals such as red yeast rice; however, in recent years, more complex and abnormal commodities such as spices and infant foods are becoming more widely assessed. The aim of this study was to develop and validate clean-up methods for spices and cereal-based infant foods using a citrinin immunoaffinity column before HPLC analysis with fluorescence detection. Each method developed was validated with a representative matrix, spiked at various citrinin concentrations, based around European Union (EU) regulations set for ochratoxin A (OTA), with recoveries >80% and % RSD < 9% in all cases. The limit of detection (LOD) and the limit of quantification (LOQ) were established at 1 and 3 µg/kg for spices and 0.1 and 0.25 µg/kg for infant cereals, respectively. These methods were then tested across a variety of spices and infant food products to establish efficacy with high recoveries >75% and % RSD < 5% across all matrices assessed. Therefore, these methods proved suitable for providing effective clean-up of spices and infant cereals, enabling reliable quantification of citrinin detected. Samples such as nutmeg and infant multigrain porridge had higher levels of citrinin contamination than anticipated, indicating that citrinin could be a concern for public health. This highlighted the need for close monitoring of citrinin contamination in these commodities, which may become regulated in the future.
Lomonosov GRB Catalogue: The First Experience of Prompt Emission Multi-Wavelength Observations
Authors: Sadovnichy V. A. , Panasyuk M. I. , Svertilov S. I. , Lipunov V. M. , Bogomolov V. V. , Bogomolov A. V. , Gorbovskoy E. S. , Iyudin A. F. , Kalegaev V. V. , Kornilov V. G. , Park I. H. , Petrov V. L. , Vedenkin N. N. , Yashin I. V.
Published: October 2021

This paper presents a catalogue of gamma-ray bursts (GRBs) that were detected by the instruments onboard the Lomonosov space observatory. The Lomonosov mission gave the first experience of not only multi-wavelength (from optical to gamma) observations of GRBs but also multi-messenger observations of extreme phenomena and GRBs. The detailed light curves and energy spectra of the detected GRBs are presented. The results of the prompt, early an afterglow optical observations of several GRBs are discussed.
Cross-Resistance of the Codling Moth against Different Isolates of Cydia pomonella Granulovirus Is Caused by Two Different but Genetically Linked Resistance Mechanisms
Authors: Sauer Annette J. , Fritsch Eva , Undorf-Spahn Karin , Iwata Kento , Kleespies Regina G. , Nakai Madoka , Jehle Johannes A.
Published: October 2021

Cydia pomonella granulovirus (CpGV) is a widely used biological control agent of the codling moth. Recently, however, the codling moth has developed different types of field resistance against CpGV isolates. Whereas type I resistance is Z chromosomal inherited and targeted at the viral gene pe38 of isolate CpGV-M, type II resistance is autosomal inherited and targeted against isolates CpGV-M and CpGV-S. Here, we report that mixtures of CpGV-M and CpGV-S fail to break type II resistance and is expressed at all larval stages. Budded virus (BV) injection experiments circumventing initial midgut infection provided evidence that resistance against CpGV-S is midgut-related, though fluorescence dequenching assay using rhodamine-18 labeled occlusion derived viruses (ODV) could not fully elucidate whether the receptor binding or an intracellular midgut factor is involved. From our peroral and intra-hemocoel infection experiments, we conclude that two different (but genetically linked) resistance mechanisms are responsible for type II resistance in the codling moth: resistance against CpGV-M is systemic whereas a second and/or additional resistance mechanism against CpGV-S is located in the midgut of CpR5M larvae.
Morphological Aspects and Viremia Analysis of BALB/c Murine Model Experimentally Infected with Dengue Virus Serotype 4
Authors: da Costa Rasinhas Arthur , Cunha Jácome Fernanda , Cardoso Caldas Gabriela , Teixeira de Almeida Ana Luisa , Nunes da Silva Marcos Alexandre , Dias Coutinho de Souza Daniel , Carlos Paulino Amanda , Mendes Bandeira Derick , Leonardo Raphael , Conrado Guerra Nunes Priscila , Mohana-Borges Ronaldo , Monika Barth Ortrud , Barreto dos Santos Flavia , Ferreira Barreto Vieira Debora
Published: October 2021

Ever since its brief introduction in the Brazilian territory in 1981, dengue virus serotype 4 (DENV-4) remained absent from the national epidemiological scenario for almost 25 years. The emergence of DENV-4 in 2010 resulted in epidemics in most Brazilian states. DENV-4, however, remains one of the least studied among the four DENV serotypes. Despite being known as a mild serotype, DENV-4 is associated with severe cases and deaths and deserves to be investigated; however, the lack of suitable experimental animal models is a limiting factor for pathogenesis studies. Here, we aimed to investigate the susceptibility and potential tropism of DENV-4 for liver, lung and heart of an immunocompetent mice model, and to evaluate and investigate the resulting morphological and ultrastructural alterations upon viral infection. BALB/c mice were inoculated intravenously with non-neuroadapted doses of DENV-4 isolated from a human case. The histopathological analysis of liver revealed typical alterations of DENV, such as microsteatosis, edema and vascular congestion, while in lung, widespread areas of hemorrhage and interstitial pneumonia were observed. While milder alterations were present in heart, characterized by limited hemorrhage and discrete presence of inflammatory infiltrate, the disorganization of the structure of the intercalated disc is of particular interest. DENV-4 RNA was detected in liver, lung, heart and serum of BALB/c mice through qRT-PCR, while the NS3 viral protein was observed in all of the aforementioned organs through immunohistochemistry. These findings indicate the susceptibility of the model to the serotype and further reinforce the usefulness of BALB/c mice in studying the many alterations caused by DENV.
Characterizing the Countrywide Epidemic Spread of Influenza A(H1N1)pdm09 Virus in Kenya between 2009 and 2018
Authors: Owuor D. Collins , de Laurent Zaydah R. , Kikwai Gilbert K. , Mayieka Lillian M. , Ochieng Melvin , Müller Nicola F. , Otieno Nancy A. , Emukule Gideon O. , Hunsperger Elizabeth A. , Garten Rebecca , Barnes John R. , Chaves Sandra S. , Nokes D. James , Agoti Charles N.
Published: October 2021

The spatiotemporal patterns of spread of influenza A(H1N1)pdm09 viruses on a countrywide scale are unclear in many tropical/subtropical regions mainly because spatiotemporally representative sequence data are lacking. We isolated, sequenced, and analyzed 383 A(H1N1)pdm09 viral genomes from hospitalized patients between 2009 and 2018 from seven locations across Kenya. Using these genomes and contemporaneously sampled global sequences, we characterized the spread of the virus in Kenya over several seasons using phylodynamic methods. The transmission dynamics of A(H1N1)pdm09 virus in Kenya were characterized by (i) multiple virus introductions into Kenya over the study period, although only a few of those introductions instigated local seasonal epidemics that then established local transmission clusters, (ii) persistence of transmission clusters over several epidemic seasons across the country, (iii) seasonal fluctuations in effective reproduction number (Re) associated with lower number of infections and seasonal fluctuations in relative genetic diversity after an initial rapid increase during the early pandemic phase, which broadly corresponded to epidemic peaks in the northern and southern hemispheres, (iv) high virus genetic diversity with greater frequency of seasonal fluctuations in 2009–2011 and 2018 and low virus genetic diversity with relatively weaker seasonal fluctuations in 2012–2017, and (v) virus spread across Kenya. Considerable influenza virus diversity circulated within Kenya, including persistent viral lineages that were unique to the country, which may have been capable of dissemination to other continents through a globally migrating virus population. Further knowledge of the viral lineages that circulate within understudied low-to-middle-income tropical and subtropical regions is required to understand the full diversity and global ecology of influenza viruses in humans and to inform vaccination strategies within these regions.
Neutralization of Dengue Virus Serotypes by Sera from Dengue-Infected Individuals Is Preferentially Directed to Heterologous Serotypes and Not against the Autologous Serotype Present in Acute Infection
Authors: Auerswald Heidi , Kann Simone , Klepsch Leonard , Hülsemann Janne , Rudnik Ines , Schreiber Sebastian , Buchy Philippe , Schreiber Michael
Published: October 2021

Sequential infections of humans by the four different dengue serotypes (DENV-1–4) lead to neutralizing antibodies with group, cross, and type specificity. Virus neutralization of serotypes showed monotypic but mostly multitypic neutralization profiles due to multiple virus exposures. We have studied neutralization to heterologous, reference DENV serotypes using paired sera collected between days 6 and 37 after onset of fever. The DENV-primed neutralization profile of the first serum sample, which was monitored by a foci reduction neutralization test (FRNT), was boosted but the neutralization profile stayed unchanged in the second serum sample. In 45 of 47 paired serum samples, the predominant neutralization was directed against DENV serotypes distinct from the infecting serotype. Homologous neutralization studies using sera and viruses from the same area, 33 secondary sera from DENV-1 infected Cambodian patients and eight virus isolates from Cambodia, showed that the FRNT assay accurately predicted the lack of a predominant antibody response against the infecting DENV-1 serotype in contrast to FRNT results using the WHO set of DENV viruses. This report provides evidence that DENV-primed multitypic neutralizing antibody profiles were mainly boosted and stayed unchanged after secondary infection and that DENV neutralization was predominantly directed to heterologous DENV but not against the infecting homologous serotype.
Alveolar-like Macrophages Attenuate Respiratory Syncytial Virus Infection
Authors: Porto Bárbara N. , Litvack Michael L. , Cen Yuchen , Lok Irene , Bouch Sheena , Norris Michael J. , Duan Wenming , Ackerley Cameron , Post Martin , Moraes Theo J.
Published: October 2021

Respiratory Syncytial Virus (RSV) is the leading cause of acute lower respiratory infections in young children and infection has been linked to the development of persistent lung disease in the form of wheezing and asthma. Despite substantial research efforts, there are no RSV vaccines currently available and an effective monoclonal antibody targeting the RSV fusion protein (palivizumab) is of limited general use given the associated expense. Therefore, the development of novel approaches to prevent RSV infection is highly desirable to improve pediatric health globally. We have developed a method to generate alveolar-like macrophages (ALMs) from pluripotent stem cells. These ALMs have shown potential to promote airway innate immunity and tissue repair and so we hypothesized that ALMs could be used as a strategy to prevent RSV infection. Here, we demonstrate that ALMs are not productively infected by RSV and prevent the infection of epithelial cells. Prevention of epithelial infection was mediated by two different mechanisms: phagocytosis of RSV particles and release of an antiviral soluble factor different from type I interferon. Furthermore, intratracheal administration of ALMs protected mice from subsequent virus-induced weight loss and decreased lung viral titres and inflammation, indicating that ALMs can impair the pathogenesis of RSV infection. Our results support a prophylactic role for ALMs in the setting of RSV infection and warrant further studies on stem cell-derived ALMs as a novel cell-based therapy for pulmonary viral infections.
Phages from Genus Bruynoghevirus and Phage Therapy: Pseudomonas Phage Delta Case
Authors: Knezevic Petar , Petrovic Fabijan Aleksandra , Gavric Damir , Pejic Jovana , Doffkay Zsolt , Rakhely Gábor
Published: October 2021

The applicability and safety of bacteriophage Delta as a potential anti-Pseudomonas aeruginosa agent belonging to genus Bruynoghevirus (family Podoviridae) was characterised. Phage Delta belongs to the species Pseudomonas virus PaP3, which has been described as a temperate, with cos sites at the end of the genome. The phage Delta possesses a genome of 45,970 bp that encodes tRNA for proline (Pro), aspartic acid (Asp) and asparagine (Asn) and does not encode any known protein involved in lysogeny formation or persistence. Analysis showed that phage Delta has 182 bp direct terminal repeats at the end of genome and lysogeny was confirmed, neither upon infection at low nor at high multiplicity of infection (MOI). The turbid plaques that appear on certain host lawns can result from bacteriophage insensitive mutants that occur with higher frequency (10−4). In silico analysis showed that the genome of Delta phage does not encode any known bacterial toxin or virulence factor, determinants of antibiotic resistance and known human allergens. Based on the broad host range and high lytic activity against planktonic and biofilm cells, phage Delta represents a promising candidate for phage therapy.
Imbalanced Immune Response of T-Cell and B-Cell Subsets in Patients with Moderate and Severe COVID-19
Authors: Golovkin Alexey , Kalinina Olga , Bezrukikh Vadim , Aquino Arthur , Zaikova Ekaterina , Karonova Tatyana , Melnik Olesya , Vasilieva Elena , Kudryavtsev Igor
Published: October 2021

Background: The immunological changes associated with COVID-19 are largely unknown. Methods: Patients with COVID-19 showing moderate (n = 18; SpO2 > 93%, respiratory rate > 22 per minute, CRP > 10 mg/L) and severe (n = 23; SpO2 < 93%, respiratory rate >30 per minute, PaO2/FiO2 ≤ 300 mmHg, permanent oxygen therapy, qSOFA > 2) infection, and 37 healthy donors (HD) were enrolled. Circulating T- and B-cell subsets were analyzed by flow cytometry. Results: CD4+Th cells were skewed toward Th2-like phenotypes within CD45RA+CD62L− (CM) and CD45RA–CD62L− (EM) cells in patients with severe COVID-19, while CM CCR6+ Th17-like cells were decreased if compared with HD. Within CM Th17-like cells “classical” Th17-like cells were increased and Th17.1-like cells were decreased in severe COVID-19 cases. Circulating CM follicular Th-like (Tfh) cells were decreased in all COVID-19 patients, and Tfh17-like cells represented the most predominant subset in severe COVID-19 cases. Both groups of patients showed increased levels of IgD-CD38++ B cells, while the levels of IgD+CD38− and IgD–CD38− were decreased. The frequency of IgD+CD27+ and IgD–CD27+ B cells was significantly reduced in severe COVID-19 cases. Conclusions: We showed an imbalance within almost all circulating memory Th subsets during acute COVID-19 and showed that altered Tfh polarization led to a dysregulated humoral immune response.
Dengue Virus Infection: A Tale of Viral Exploitations and Host Responses
Authors: Nanaware Nikita , Banerjee Anwesha , Mullick Bagchi Satarupa , Bagchi Parikshit , Mukherjee Anupam
Published: October 2021

Dengue is a mosquito-borne viral disease (arboviral) caused by the Dengue virus. It is one of the prominent public health problems in tropical and subtropical regions with no effective vaccines. Every year around 400 million people get infected by the Dengue virus, with a mortality rate of about 20% among the patients with severe dengue. The Dengue virus belongs to the Flaviviridae family, and it is an enveloped virus with positive-sense single-stranded RNA as the genetic material. Studies of the infection cycle of this virus revealed potential host targets important for the virus replication cycle. Here in this review article, we will be discussing different stages of the Dengue virus infection cycle inside mammalian host cells and how host proteins are exploited by the virus in the course of infection as well as how the host counteracts the virus by eliciting different antiviral responses.
Cell Fusion and Syncytium Formation in Betaherpesvirus Infection
Authors: Tang Jiajia , Frascaroli Giada , Zhou Xuan , Knickmann Jan , Brune Wolfram
Published: October 2021

Cell–cell fusion is a fundamental and complex process that occurs during reproduction, organ and tissue growth, cancer metastasis, immune response, and infection. All enveloped viruses express one or more proteins that drive the fusion of the viral envelope with cellular membranes. The same proteins can mediate the fusion of the plasma membranes of adjacent cells, leading to the formation of multinucleated syncytia. While cell–cell fusion triggered by alpha- and gammaherpesviruses is well-studied, much less is known about the fusogenic potential of betaherpesviruses such as human cytomegalovirus (HCMV) and human herpesviruses 6 and 7 (HHV-6 and HHV-7). These are slow-growing viruses that are highly prevalent in the human population and associated with several diseases, particularly in individuals with an immature or impaired immune system such as fetuses and transplant recipients. While HHV-6 and HHV-7 are strictly lymphotropic, HCMV infects a very broad range of cell types including epithelial, endothelial, mesenchymal, and myeloid cells. Syncytia have been observed occasionally for all three betaherpesviruses, both during in vitro and in vivo infection. Since cell–cell fusion may allow efficient spread to neighboring cells without exposure to neutralizing antibodies and other host immune factors, viral-induced syncytia may be important for viral dissemination, long-term persistence, and pathogenicity. In this review, we provide an overview of the viral and cellular factors and mechanisms identified so far in the process of cell–cell fusion induced by betaherpesviruses and discuss the possible consequences for cellular dysfunction and pathogenesis.
Best Molecular Tools to Investigate Coronavirus Diversity in Mammals: A Comparison
Authors: Drzewnioková Petra , Festa Francesca , Panzarin Valentina , Lelli Davide , Moreno Ana , Zecchin Barbara , De Benedictis Paola , Leopardi Stefania
Published: October 2021

Coronaviruses (CoVs) are widespread and highly diversified in wildlife and domestic mammals and can emerge as zoonotic or epizootic pathogens and consequently host shift from these reservoirs, highlighting the importance of veterinary surveillance. All genera can be found in mammals, with α and β showing the highest frequency and diversification. The aims of this study were to review the literature for features of CoV surveillance in animals, to test widely used molecular protocols, and to identify the most effective one in terms of spectrum and sensitivity. We combined a literature review with analyses in silico and in vitro using viral strains and archive field samples. We found that most protocols defined as pan-coronavirus are strongly biased towards α- and β-CoVs and show medium-low sensitivity. The best results were observed using our new protocol, showing LoD 100 PFU/mL for SARS-CoV-2, 50 TCID50/mL for CaCoV, 0.39 TCID50/mL for BoCoV, and 9 ± 1 log2 ×10−5 HA for IBV. The protocol successfully confirmed the positivity for a broad range of CoVs in 30/30 field samples. Our study points out that pan-CoV surveillance in mammals could be strongly improved in sensitivity and spectrum and propose the application of a new RT-PCR assay, which is able to detect CoVs from all four genera, with an optimal sensitivity for α-, β-, and γ-.