bluuebunny/crossref_metadata_2025 huggingface dataset:
Rlip Depletion Alters Oncogene Transcription at Multiple Distinct Regulatory Levels
Authors: Hindle Ashly , Bose Chhanda , Lee Jihyun , Palade Philip T. , Peterson Christopher J. , Reddy P. Hemachandra , Awasthi Sanjay , Singh Sharda P.
Published: January 2022

Rlip76 (Rlip) is a multifunctional membrane protein that facilitates the high metabolic rates of cancer cells through the efflux of toxic metabolites and other functions. Rlip inhibition or depletion results in broad-spectrum anti-cancer effects in vitro and in vivo. Rlip depletion effectively suppresses malignancy and causes global reversion of characteristic CpG island methylomic and transcriptomic aberrations in the p53-null mouse model of spontaneous carcinogenesis through incompletely defined signaling and transcriptomic mechanisms. The methylome and transcriptome are normally regulated by the concerted actions of several mechanisms that include chromatin remodeling, promoter methylation, transcription factor interactions, and miRNAs. The present studies investigated the interaction of Rlip depletion or inhibition with the promoter methylation and transcription of selected cancer-related genes identified as being affected by Rlip depletion in our previous studies. We constructed novel promoter CpG island/luciferase reporter plasmids that respond only to CpG methylation and transcription factors. We found that Rlip depletion regulated expression by a transcription factor-based mechanism that functioned independently of promoter CpG methylation, lipid peroxidation, and p53 status.
The Obscure Potential of AHNAK2
Authors: Zardab Mohamed , Stasinos Konstantinos , Grose Richard P. , Kocher Hemant M.
Published: January 2022

AHNAK2 is a protein discovered in 2004, with a strong association with oncogenesis in various epithelial cancers. It has a large 616 kDa tripartite structure and is thought to take part in the formation of large multi-protein complexes. High expression is found in clear cell renal carcinoma, pancreatic ductal adenocarcinoma, uveal melanoma, and lung adenocarcinoma, with a relation to poor prognosis. Little work has been done in exploring the function and relation AHNAK2 has with cancer, with early studies showing promising potential as a future biomarker and therapeutic target.
Exploration of Extracellular Vesicle miRNAs, Targeted mRNAs and Pathways in Prostate Cancer: Relation to Disease Status and Progression
Authors: Puhka Maija , Thierens Lisse , Nicorici Daniel , Forsman Tarja , Mirtti Tuomas , af Hällström Taija , Serkkola Elina , Rannikko Antti
Published: January 2022

Background: Prostate cancer (PCa) lacks non-invasive specific biomarkers for aggressive disease. We studied the potential of urinary extracellular vesicles (uEV) as a liquid PCa biopsy by focusing on the micro RNA (miRNA) cargo, target messenger RNA (mRNA) and pathway analysis. Methods: We subjected uEV samples from 31 PCa patients (pre-prostatectomy) to miRNA sequencing and matched uEV and plasma EV (pEV) from three PCa patients to mRNA sequencing. EV quality control was performed by electron microscopy, Western blotting and particle and RNA analysis. We compared miRNA expression based on PCa status (Gleason Score) and progression (post-prostatectomy follow-up) and confirmed selected miRNAs by quantitative PCR. Expression of target mRNAs was mapped in matched EV. Results: Quality control showed typical small uEV, pEV, RNA and EV-protein marker enriched samples. Comparisons between PCa groups revealed mostly unique differentially expressed miRNAs. However, they targeted comprehensive and largely overlapping sets of cancer and progression-associated signalling, resistance, hormonal and immune pathways. Quantitative PCR confirmed changes in miR-892a (Gleason Score 7 vs. ≥8), miR-223-3p (progression vs. no progression) and miR-146a-5p (both comparisons). Their target mRNAs were expressed widely in PCa EV. Conclusions: PCa status and progression-linked RNAs in uEV are worth exploration in large personalized medicine trials.
Targeting Chronic Inflammation of the Digestive System in Cancer Prevention: Modulators of the Bioactive Sphingolipid Sphingosine-1-Phosphate Pathway
Authors: McGowan Eileen M. , Lin Yiguang , Chen Size
Published: January 2022

Incidence of gastrointestinal (GI) cancers is increasing, and late-stage diagnosis makes these cancers difficult to treat. Chronic and low-grade inflammation are recognized risks for most GI cancers. The GI mucosal immune system maintains healthy homeostasis and signalling molecules made from saturated fats, bioactive sphingolipids, play essential roles in healthy GI immunity. Sphingosine-1-phosphate (S1P), a bioactive sphingolipid, is a key mediator in a balanced GI immune response. Disruption in the S1P pathway underlies systemic chronic metabolic inflammatory disorders, including diabetes and GI cancers, providing a strong rationale for using modulators of the S1P pathway to treat pathological inflammation. Here, we discuss the effects of bioactive sphingolipids in immune homeostasis with a focus on S1P in chronic low-grade inflammation associated with increased risk of GI carcinogenesis. Contemporary information on S1P signalling involvement in cancers of the digestive system, from top to bottom, is reviewed. Further, we discuss the use of novel S1P receptor modulators currently in clinical trials and their potential as first-line drugs in the clinic for chronic inflammatory diseases. Recently, ozanimod (ZeposiaTM) and etrasimod have been approved for clinical use to treat ulcerative colitis and eosinophilic oesophagitis, respectively, which may have longer term benefits in reducing risk of GI cancers.
The Good, the Bad, and the Ugly: Neutrophils, Angiogenesis, and Cancer
Authors: Ozel Irem , Duerig Inga , Domnich Maksim , Lang Stephan , Pylaeva Ekaterina , Jablonska Jadwiga
Published: January 2022

Angiogenesis, the formation of new blood vessels from already existing vasculature, is tightly regulated by pro- and anti-angiogenic stimuli and occurs under both physiological and pathological conditions. Tumor angiogenesis is central for tumor development, and an “angiogenic switch” could be initiated by multiple immune cells, such as neutrophils. Tumor-associated neutrophils promote tumor angiogenesis by the release of both conventional and non-conventional pro-angiogenic factors. Therefore, neutrophil-mediated tumor angiogenesis should be taken into consideration in the design of novel anti-cancer therapy. This review recapitulates the complex role of neutrophils in tumor angiogenesis and summarizes neutrophil-derived pro-angiogenic factors and mechanisms regulating angiogenic activity of tumor-associated neutrophils. Moreover, it provides up-to-date information about neutrophil-targeting therapy, complementary to anti-angiogenic treatment.
Multifaceted Interplay between Hormones, Growth Factors and Hypoxia in the Tumor Microenvironment
Authors: Lappano Rosamaria , Todd Lauren A. , Stanic Mia , Cai Qi , Maggiolini Marcello , Marincola Francesco , Pietrobon Violena
Published: January 2022

Hormones and growth factors (GFs) are signaling molecules implicated in the regulation of a variety of cellular processes. They play important roles in both healthy and tumor cells, where they function by binding to specific receptors on target cells and activating downstream signaling cascades. The stages of tumor progression are influenced by hormones and GF signaling. Hypoxia, a hallmark of cancer progression, contributes to tumor plasticity and heterogeneity. Most solid tumors contain a hypoxic core due to rapid cellular proliferation that outgrows the blood supply. In these circumstances, hypoxia-inducible factors (HIFs) play a central role in the adaptation of tumor cells to their new environment, dramatically reshaping their transcriptional profile. HIF signaling is modulated by a variety of factors including hormones and GFs, which activate signaling pathways that enhance tumor growth and metastatic potential and impair responses to therapy. In this review, we summarize the role of hormones and GFs during cancer onset and progression with a particular focus on hypoxia and the interplay with HIF proteins. We also discuss how hypoxia influences the efficacy of cancer immunotherapy, considering that a hypoxic environment may act as a determinant of the immune-excluded phenotype and a major hindrance to the success of adoptive cell therapies.
An Overview of Therapy Guidelines for Cardiac Arrest and the Potential Benefits of Hemoglobin-Based Oxygen Carriers
Authors: Wollocko Brian M. , Papian-Gorji Bardia , Yen Winston , Zahid Urooj , Shah Nilank , Steier Kenneth , Wollocko Hanna
Published: January 2022

Currently, there is an unmet therapeutic need for the medical management of cardiac arrest, as is evident from the high mortality rate associated with this condition. These dire outcomes can be attributed to the severe nature and poor prognosis of this disorder. However, the current treatment modalities, while helping to augment survival, are limited and do not offer adequate improvements to outcomes. Treatment modalities are particularly lacking when considering the underlying pathophysiology of the metabolic phase of cardiac arrest. In this study, we explore the three phases of cardiac arrest and assess the factors related to positive clinical outcomes and survival for these events. Furthermore, we evaluate the present guidelines for resuscitation and recovery, the issues related to ischemia and tissue reperfusion, and the benefit of oxygen-delivery therapeutic methods including blood transfusion therapy and synthetic hemoglobins (HBOCs). The current therapy protocols are limited specifically by the lack of an efficient method of oxygen delivery to address the metabolic phase of cardiac arrest. In this article, we investigate the next generation of HBOCs and review their properties that make them attractive for their potential application in the treatment of cardiac arrest. These products may be a viable solution to address complications associated with ischemia, reperfusion injury, and organ damage.
En Route to CO2-Based (a)Cyclic Carbonates and Polycarbonates from Alcohols Substrates by Direct and Indirect Approaches
Authors: Brege Antoine , Grignard Bruno , Méreau Raphaël , Detrembleur Christophe , Jerome Christine , Tassaing Thierry
Published: January 2022

This review is dedicated to the state-of-the art routes used for the synthesis of CO2-based (a)cyclic carbonates and polycarbonates from alcohol substrates, with an emphasis on their respective main advantages and limitations. The first section reviews the synthesis of organic carbonates such as dialkyl carbonates or cyclic carbonates from the carbonation of alcohols. Many different synthetic strategies have been reported (dehydrative condensation, the alkylation route, the “leaving group” strategy, the carbodiimide route, the protected alcohols route, etc.) with various substrates (mono-alcohols, diols, allyl alcohols, halohydrins, propargylic alcohols, etc.). The second section reviews the formation of polycarbonates via the direct copolymerization of CO2 with diols, as well as the ring-opening polymerization route. Finally, polycondensation processes involving CO2-based dimethyl and diphenyl carbonates with aliphatic and aromatic diols are described.
Increased Numbers of Enteric Glial Cells in the Peyer’s Patches and Enhanced Intestinal Permeability by Glial Cell Mediators in Patients with Ileal Crohn’s Disease
Authors: Biskou Olga , Meira de-Faria Felipe , Walter Susanna M. , Winberg Martin E. , Haapaniemi Staffan , Myrelid Pär , Söderholm Johan D. , Keita Åsa V.
Published: January 2022

Enteric glial cells (EGC) are known to regulate gastrointestinal functions; however, their role in Crohn’s disease (CD) is elusive. Microscopic erosions over the ileal Peyer’s patches are early signs of CD. The aim of this work was to assess the localization of EGC in the follicle and interfollicular region of the Peyer’s patches and in the lamina propria and study the effects of EGC mediators on barrier function in CD patients and non-inflammatory bowel disease (non-IBD) controls. EGC markers, glial fibrillary acidic protein (GFAP), and S100 calcium-binding protein β (S100β) were quantified by immunofluorescence and Western blotting. Both markers showed significantly more EGC in the Peyer’s patches and lamina propria of CD patients compared to the non-IBD controls. In CD patients there were significantly more EGC in Peyer’s patches compared to lamina propria, while the opposite pattern was seen in controls. Barrier function studies using Ussing chambers showed increased paracellular permeability by EGC mediators in CD patients, whereas permeability decreased by the mediators in controls. We show the accumulation of EGC in Peyer’s patches of CD patients. Moreover, EGC mediators induced barrier dysfunction in CD patients. Thus, EGC might have harmful impacts on ongoing inflammation and contribute to the pathophysiology of the disease.
Interleukine-17 Modulates Neurogenesis and Behavior Following Exposure to Trauma in Mice
Authors: Willinger Yehoshua , Turgeman Gadi
Published: January 2022

Post-traumatic stress disorder (PTSD) is a psychiatric disorder accompanied by deficits in cognitive and social skills. Adult hippocampal neurogenesis is a lifelong phenomenon, with new neurons being formed in the granular cell layer of the dentate gyrus. Impaired neurogenesis is associated with multiple behavioral disorders including Alzheimer’s disease and schizophrenia. PTSD patients often present hippocampal atrophy and animal models clearly present impaired neurogenesis. Previous studies on PTSD patients demonstrated elevated levels of Th17 cells and plasma levels of the pro-inflammatory cytokine interleukin-17A (IL-17A). Since IL-17A can impair neurogenesis in mice, we thus hypothesized that decreasing the serum levels of IL-17A will increase hippocampal neurogenesis and alleviate symptoms in a murine model of PTSD. Surprisingly, our results showed that attempting to neutralize IL-17A with an antibody resulted in increased serum levels of IL-17A, while targeting IL-23, the upstream regulator of IL-17, did lower the levels of IL-17A in trauma-exposed mice. As expected, increased levels of serum IL-17A (in anti-IL-17A treated mice) resulted in impaired neurogenesis, reflected by reduced number of proliferating Ki67+ neural progenitors and newly formed DCX+ neurons, which was correlated with increased expression of Hes1. Nevertheless, increased maturation was noted by the expression of Slit2 and Ache. In contrast, treatment with anti-IL-23 indeed resulted in increased neurogenesis. Behaviorally, both treatments did not affect trauma-related freezing behavior but did affect trauma-related social deficits. Unexpectedly, increased levels of serum IL-17A (in anti-IL-17A treated mice) prevented social deficits in trauma-exposed mice while anti-IL-23 exacerbated these deficits. We thus conclude that IL-17 is involved in regulating neurogenesis following exposure to stress but may be important in maintaining social behavior.
Current Methods and Pipelines for Image-Based Quantitation of Nuclear Shape and Nuclear Envelope Abnormalities
Authors: Janssen Anne F. J. , Breusegem Sophia Y. , Larrieu Delphine
Published: January 2022

Any given cell type has an associated “normal” nuclear morphology, which is important to maintain proper cellular functioning and safeguard genomic integrity. Deviations from this can be indicative of diseases such as cancer or premature aging syndrome. To accurately assess nuclear abnormalities, it is important to use quantitative measures of nuclear morphology. Here, we give an overview of several nuclear abnormalities, including micronuclei, nuclear envelope invaginations, blebs and ruptures, and review the current methods used for image-based quantification of these abnormalities. We discuss several parameters that can be used to quantify nuclear shape and compare their outputs using example images. In addition, we present new pipelines for quantitative analysis of nuclear blebs and invaginations. Quantitative analyses of nuclear aberrations and shape will be important in a wide range of applications, from assessments of cancer cell anomalies to studies of nucleus deformability under mechanical or other types of stress.
Homologous Recombination-Enhancing Factors Identified by Comparative Transcriptomic Analyses of Pluripotent Stem Cell of Human and Common Marmoset
Authors: Yoshimatsu Sho , Nakajima Mayutaka , Qian Emi , Sanosaka Tsukasa , Sato Tsukika , Okano Hideyuki
Published: January 2022

A previous study assessing the efficiency of the genome editing technology CRISPR-Cas9 for knock-in gene targeting in common marmoset (marmoset; Callithrix jacchus) embryonic stem cells (ESCs) unexpectedly identified innately enhanced homologous recombination activity in marmoset ESCs. Here, we compared gene expression in marmoset and human pluripotent stem cells using transcriptomic and quantitative PCR analyses and found that five HR-related genes (BRCA1, BRCA2, RAD51C, RAD51D, and RAD51) were upregulated in marmoset cells. A total of four of these upregulated genes enhanced HR efficiency with CRISPR-Cas9 in human pluripotent stem cells. Thus, the present study provides a novel insight into species-specific mechanisms for the choice of DNA repair pathways.
The Role of the Lysosomal Cl−/H+ Antiporter ClC-7 in Osteopetrosis and Neurodegeneration
Authors: Zifarelli Giovanni
Published: January 2022

CLC proteins comprise Cl− channels and anion/H+ antiporters involved in several fundamental physiological processes. ClC-7 is a lysosomal Cl−/H+ antiporter that together with its beta subunit Ostm1 has a critical role in the ionic homeostasis of lysosomes and of the osteoclasts’ resorption lacuna, although the specific underlying mechanism has so far remained elusive. Mutations in ClC-7 cause osteopetrosis, but also a form of lysosomal storage disease and neurodegeneration. Interestingly, both loss-of- and gain-of-function mutations of ClC-7 can be pathogenic, but the mechanistic implications of this finding are still unclear. This review will focus on the recent advances in our understanding of the biophysical properties of ClC-7 and of its role in human diseases with a focus on osteopetrosis and neurodegeneration.
Remote and Technology-Based Dialogic Development during the COVID-19 Pandemic: Positive and Negative Experiences, Challenges, and Learnings
Authors: Syvänen Sirpa , Loppela Kaija
Published: January 2022

This study aimed to analyze the challenges, learning experiences, and effects of the COVID-19 pandemic in a social, healthcare, and special education development project financed by the European Social Fund. The theoretical framework of the project relied on the theories of dialogic development and leadership. The method was participatory action research using data collected from various assessments and a questionnaire. Reports of neutral, negative, and positive experiences among two participant groups of the project—the implementers and pilot organizations—in remote work, devices and applications, and remote and technology-based development processes, were recorded. Both participant groups reported increased pressure at work, social isolation, professional loneliness, and improved work control and efficiency. The pilots have learned the development method, and development has been able to continue by utilizing technology despite the pandemic. Development through technology was more difficult, and new dialogic interaction tools have been created. The project was most effective with regard to leadership, teams, renewal, and information flow. There is a need for wide-ranging dialogues with various working life actors when outlining the ways in which future work will be carried out and to reflect on how remote work, technology, and digitalization affect well-being at work, social relations, and leadership.
Comparative Genome Analysis Reveals Accumulation of Single-Nucleotide Repeats in Pathogenic Escherichia Lineages
Authors: Ishiya Koji , Nakashima Nobutaka
Published: January 2022

Homopolymeric tracts (HPTs) can lead to phase variation and DNA replication slippage, driving adaptation to environmental changes and evolution of genes and genomes. However, there is limited information on HPTs in Escherichia; therefore, we conducted a comprehensive cross-strain search for HPTs in Escherichia genomes. We determined the HPT genomic distribution and identified a pattern of high-frequency HPT localization in pathogenic Escherichia lineages. Notably, HPTs localized near transcriptional regulatory genes. Additionally, excessive repeats accumulated in toxin-coding genes. Moreover, the genomic localization of some HPTs might be derived from exogenous DNA, such as that of bacteriophages. Altogether, our findings may prove useful for understanding the role of HPTs in Escherichia genomes.
Experimental Study on the Minimum Required Specimen Width to Maximum Particle Size Ratio in Direct Shear Tests
Authors: Deiminiat Akram , Li Li , Zeng Feitao
Published: January 2022

Conducting laboratory direct shear tests on granular materials is a common practice in geotechnical engineering. This is usually done by following the ASTM D3080/D3080M-11 (hereafter named ASTM), which stipulates a minimum required value of 10 for specimen width (W) to the maximum particle size (dmax) ratio. Recently, a literature review performed by the authors showed that the minimum required W/dmax ratio given in the ASTM is not large enough to eliminate the specimen size effect (SSE). The minimum required W/dmax ratio of ASTM needs to be revised. In this study, a critical analysis is first made on existing data in order to identify the minimum required W/dmax ratio. The analysis shows that more experimental data obtained on specimens having W/dmax ratios between 10 and 50 are particularly necessary. To complete this need, a series of direct shear tests were performed on specimens having different dmax by using three shear boxes of different dimensions. The results show once again that the minimum required W/dmax ratio of 10, defined in the ASTM, is not large enough to eliminate the SSE. Further analysis on these and existing experimental results indicates that the minimum required W/dmax ratio to remove the SSE of friction angles is about 60. These results along with the limitations of this study are discussed.
Longitudinal Magneto-Optical Kerr Effect of Nanoporous CoFeB and W/CoFeB/W Thin Films
Authors: Zhang Weiwei , Chen Zhanghua , Belotelov Vladimir , Song Yujun
Published: January 2022

Nanoporous Co40Fe40B20 (CoFeB) and sandwich tungsten (W)/CoFeB/W thin films were fabricated via an anodic aluminum oxide (AAO) template-assisted magneto sputtering process. Their thickness-dependent magneto-optical Kerr effect (MOKE) hysteresis loops were investigated for enhanced Kerr rotation. Control of the Kerr null points of the polarized reflected light can be realized via the thicknesses of the CoFeB layers and W layers. Simulation of the thickness-dependent phase difference change by the finite element method reveals the existence of the two Kerr null points for W/CoFeB/W thin films, matching the experimental result very well. However, there are two additional Kerr null points for pure CoFeB thin films according to the simulation by comparing with the experimental result (only one). Theoretical analysis indicates that the different Kerr null points between the experimental result and the simulation are mainly due to the enhanced inner magnetization in the ferromagnetic CoFeB layer with the increased thickness, which is usually omitted in the simulation. Clearly, the introduction of non-ferromagnetic W layers can experimentally regulate the Kerr null points of ferromagnetic thin films. Moreover, construction of W/CoFeB/W sandwich thin films can greatly increase the highest magneto-optical susceptibility and the saturated Kerr rotation angle when compared with CoFeB thin films of the same thickness.
Microstructure Evolution of Mg-Sn-Y Alloy Solidified under High Pressure and Temperature Gradient
Authors: Zou Chunming , Zhang Rong , Wei Zunjie , Wang Hongwei
Published: January 2022

The microstructures of Mg-1Sn-2.5Y (wt%) alloys solidified under high pressures were investigated. In addition, a mathematical model was established to analyze the effects of solidification pressure and cooling rate on the average grain size. The results show that the alloy was solidified under high pressure and temperature gradient using the cooling rate difference in the high pressure chamber, resulting in the formation of the outer equiaxed zone, the columnar zone, and the equiaxed zone in the sample. With an increase in the solidification pressure, the columnar-to-equiaxed transition was inhibited in Mg-1Sn-2.5Y alloy. In the outer fine equiaxed zone and the columnar zone, the solubility of Sn in the Mg matrix increased with an increase in solidification pressure. The average secondary dendrite arm spacing decreased from 14–17 μm under 1 GPa to 9–11 μm under 1.5 GPa. Increases in pressure and cooling rate resulted in a reduction in average grain size.
Performance and Hydration Mechanism of Modified Tabia with Composite-Activated Coal Gangue
Authors: Zhao Yanbing , Yang Caiqian , Cheng Songlin , Wu Zhiren , Wang Bokun
Published: January 2022

The feasibility of modified tabia (MT) with composite-activated coal gangue (CACG) as the subgrade material of low-grade highways was experimentally investigated. A composite activation method was employed to improve the pozzolanic activity of coal gangue. The effect of CACG content on the mechanical properties of MT was investigated through a series of experiments. It was found that the pozzolanic reactivity of coal gangue was remarkably enhanced by the composite activation method. Compared with traditional tabia (TT), the unconfined compressive strength, splitting strength, and flexural tensile strength of the MT with 50% of CACG content increased by 5.03 times, 9.71 times, and 1.50 times, respectively. The impermeability of specimens with CACG significantly improved. Furthermore, the mass loss rate of MT was less than 2.83%, while it reached up to 34.20% in TT after being conditioned to 40 freeze–thaw cycles. Finally, the microstructure change and hydration mechanism of MT are discussed and revealed.
Microstructure Characterization and Battery Performance Comparison of MOF-235 and TiO2-P25 Materials
Authors: Zhao Zilong , Jiang Xiaowei , Li Sirui , Li Liang , Feng Zhiyuan , Lai Huansheng
Published: January 2022

The growing interest in energy storage has led to the urgent need for the development of high-performance cathode electrodes. The commercialized materials MOF-235 and TiO2-P25 exhibit characteristics that may be suitable as electrodes but there are inherent challenges that have yet to be addressed in the literature. In this study, a high-pressure hydrothermal synthesized MOF-235 and sol-gel-made TiO2-P25 were tested for battery performance. The results indicate that MOF-235 does not possess the desired performance due to uncontrollable agglomeration. On the other hand, TiO2-P25 showed good cycling life, and the performance can be further optimized by doping and minimizing the particle size. Additionally, SEM and TEM were applied for surface characterization, providing evidence that mesoporous TiO2-25 inhibits photo-generated carrier recombination. The mesoporous energy storage mechanism of those two materials is also discussed. This research will provide technical support for the industrialization of those two mesoporous materials.
Mitochondrial Genetic Diversity and Structure of the Langur Population in a Complex Landscape of the Nepal Himalaya
Authors: Khanal Laxman , Chalise Mukesh Kumar , Jiang Xue-Long , Kyes Randall C.
Published: January 2022

Heterogenous landscape features of the Himalayan region shape the genetic structure of animal populations by delimiting spatial patterns of dispersal and reproduction. Integrating population genetic analysis with landscape features could yield results that shed light on the evolutionary diversity of the taxa therein. This study assessed the population genetic structure of the Nepal Himalayan langurs (Semnopithecus spp.) across almost their entire distribution range in the complex landscape of the Nepal Himalaya using the mitochondrial cytochrome b (CYTB, 1140 bp), cytochrome c oxidase I (COI, 676 bp), and control region (1088 bp) sequences. Sequences were successfully retrieved from 52 samples belonging to 17 troops of wild Himalayan langurs in Nepal. The concatenated alignment of the three loci (2904 bp) defined 35 unique haplotypes with haplotype and nucleotide diversities of 0.961 ± 0.017 and 0.0204 ± 0.004, respectively. The results of a median joining haplotype network and of inter-haplotypic phylogenetic analyses revealed five major clades across the country: one from the eastern, two from the central, and two from the western region of Nepal. No haplotypes were shared among the regions. The Mantel test results indicated that the landscape heterogeneity of the Himalaya has shaped the population genetic structure of the Himalayan langurs due to the combined effects of isolation by resistance and isolation by distance phenomena. The strong population genetic structure and deep mtDNA divergence warrants a detailed taxonomic assessment of the Himalayan langurs across their entire range.
Morphology and Molecular Phylogeny of Fuscheriides baugilensis sp. nov. (Protozoa, Ciliophora, Haptorida) from South Korea
Authors: Jang Seok Won , Omar Atef , Nam Seung Won , Jung Jae-Ho
Published: January 2022

The morphology and molecular phylogeny of a new haptorid ciliate, Fuscheriides baugilensis sp. nov., discovered in a temporary pond in South Korea, were investigated. The new species is characterized by its small body size (30–55 × 15–20 μm in vivo), oblong to rod-shaped extrusomes in the oral bulge and cytoplasm, 14–16 somatic kineties, two dorsal brush rows, and single subapical ciliary condensation. The phylogenetic analyses based on the 18S rRNA gene sequences show that the family Fuscheriidae is paraphyletic and the species belong to the genera Fuscheriides and Pseudofuscheria cluster together in the same subclade, while Fuscheria is in a different subclade, suggesting that the subapical ciliary condensation characterizing the two former genera has a higher taxonomic value than the shape of extrusomes for genera separation.
Structure and Characteristics of Plant-Frugivore Network in an Urban Park: A Case Study in Nanjing Botanical Garden Mem. Sun Yat-Sen
Authors: Zhang Manyu , Lu Caihong , Han Qian , Lu Changhu
Published: January 2022

Interaction between animals and plants is an important way to maintain terrestrial biodiversity and ecosystem function, but little is known about the structure of reciprocal networks between fruit plants and frugivore birds in urban habitats. To explore the characteristics of the plant–frugivore network and network roles of species, we observed the fruit ripening phenology of 21 species of fruit plants and the interactions between these plants and 39 species of frugivore birds during the whole year in a large urban park. Then, we assessed the characteristics of the total plant–frugivore and seasonal networks, and analyzed the network roles of species and the relationship between their network roles and ecological traits. Fruit ripened mainly in autumn and winter, and interaction connections in the two seasons contributed 39.68% and 44.83% to the total network, respectively. The specialization (H2′), connectance (C), nestedness, and interaction evenness (eH2) of the network were lower in autumn and winter, while the interaction diversity was higher. Compared with the networks (N = 1000) generated by the null model, the observed network exhibited higher nestedness and interaction diversity (E2) and lower specialization (H2′), connectance (C), and interaction evenness (eH2). A correlation analysis combining ecological traits and network roles showed that plants with black fruit had higher effective partners and partner diversity, while other traits of plants and birds were not significantly correlated with their network parameters. The important plants (N = 6) and birds (N = 3) contributed to 71.78% and 67.55% of the total network connection, they were mainly evergreen plants with black and red drupes and omnivorous generalist birds with medium and large sizes. Our research highlighted the seasonal differences in urban plant–frugivore network and the value of important species in maintaining network structures and providing ecosystem services.
Biomechanical Device for Measurement of Adductors Strength and Aid in Self-Catheterisation of Spastic Patients
Authors: Cruz Maria , Maranha Vítor , Moita Fernando , Cruz Nuno , Rasteiro Deolinda , Carvalho Filipe , Lains Jorge , Roseiro Luis
Published: January 2022

Intermittent vesical self-catheterisation is a legitimate and safe technique that has been reported since the 1970s as a solution for the treatment and prevention of vesical urinary complications resulting from spinal cord injury. This practice, using clean technology, has been asserting itself as one of the best alternatives for people with neurogenic bladder. However, adherence is not complete due to some barriers imposed to this procedure by the injured, with emphasis on positioning, agility, and visual impairment. The solutions presented today to support self-catheterisation are expensive equipment that does not allow patients with advanced levels of spasticity to have their autonomy. A biomechanical support device was developed to aid self-catheterisation, mainly aimed at women with spasticity, filling the gap in the existing products. Despite the main objective of self-catheterisation, the system’s design made it possible to quantify the strength of the adductors for the sitting position during the execution of the adduction movement, particularly relevant for spastic patients. The device’s production was entirely carried out using the FDM methodology, with 3D printers, and its design and operation were thought to overcome the physical and psychological barriers imposed by the users. The system was first tested with a group of healthy volunteers to obtain a pattern of the adductors force in a sitting position and after with a group of spastic volunteers. The obtained data allows to compare the adductor force data and optimize the system, with particular functionalities for spastic patients, with the implementation of a motorised version and a visualization camera. The system, its developments, and results obtained are present and discussed.
Multiplex Immunofluorescence Assay of Infiltrating Mononu-Clear Cell Subsets in Acute T-Cell-Mediated Rejection and BK Virus-Associated Nephropathy in the Allograft Kidney
Authors: Kim Mee-Seon , Lim Jeong-Hoon , Han Man-Hoon , Kim Sang-Yeob , Kim Yun Jae , Kim Yong-Jin
Published: January 2022

Renal allograft biopsy is the gold standard procedure for diagnosis of kidney rejection via specific pathological changes. To provide a better assessment of immunologic events in acute T-cell-mediated rejection (acute TCMR) and BK virus-associated nephropathy (BKVAN) cases, we used multiplex immunofluorescence staining to identify infiltrating mononuclear cell subsets in the cortex area of transplanted kidneys. Antibodies to CD4, CD8, CD20, CD68, Foxp3, and cytokeratin were used. In cortical interstitium, CD8+ cells were significantly more prevalent in acute TCMR than BKVAN cases (34% vs. 22.8%, p = 0.034). In medulla, CD20+ cells were significantly more prevalent in BKVAN than acute TCMR cases (51.9% vs. 11.3%, p = 0.028).
Iron in Porphyrias: Friend or Foe?
Authors: Buzzetti Elena , Ventura Paolo , Corradini Elena
Published: January 2022

Iron is a trace element that is important for many vital processes, including oxygen transport, oxidative metabolism, cellular proliferation, and catalytic reactions. Iron supports these functions mainly as part of the heme molecule. Heme synthesis is an eight-step process which, when defective at the level of one of the eight enzymes involved, can cause the development of a group of diseases, either inherited or acquired, called porphyrias. Despite the strict link between iron and heme, the role of iron in the different types of porphyrias, particularly as a risk factor for disease development/progression or as a potential therapeutic target or molecule, is still being debated, since contrasting results have emerged from clinical observations, in vitro studies and animal models. In this review we aim to deepen such aspects by drawing attention to the current evidence on the role of iron in porphyrias and its potential implication. Testing for iron status and its metabolic pathways through blood tests, imaging techniques or genetic studies on patients affected by porphyrias can provide additional diagnostic and prognostic value to the clinical care, leading to a more tailored and effective management.
Troponin Cut-Offs for Acute Myocardial Infarction in Patients with Impaired Renal Function—A Systematic Review and Meta-Analysis
Authors: Kampmann Jan , Heaf James , Backer Mogensen Christian , Pedersen Andreas Kristian , Granhøj Jeff , Mickley Hans , Brandt Frans
Published: January 2022

Identifying acute myocardial infarction in patients with renal disease is notoriously difficult, due to atypical presentation and chronically elevated troponin. The aim of this study was to identify a specific troponin T/troponin I cut-off value for diagnosis of acute myocardial infarction in patients with renal impairment via meta-analysis. Two investigators screened 2590 publications from MEDLINE, Embase, PubMed, Web of Science, and the Cochrane library. Only studies that investigated alternative cut-offs according to renal impairment were included. Fifteen articles fulfilled the inclusion criteria. Six studies were combined for meta-analysis. The manufacturer’s upper reference level for troponin T is 14 ng/L. Based on the meta-analyses, cut-off values for troponin in patients with renal impairment with myocardial infarction was 42 ng/L for troponin I and 48 ng/L for troponin T. For patients on dialysis the troponin T cut-off is even higher at 239 ng/L. A troponin I cut-off value for dialysis patients could not be established due to lack of data. The 15 studies analyzed showed considerable diversity in study design, study population, and the definition of myocardial infarction. Further studies are needed to define a reliable troponin cut-off value for patients with kidney disease, especially in dialysis patients, and to allow necessary subanalysis.
Transforming a Didactic Lecture into a Student-Centered Active Learning Exercise—Teaching Equine Diarrhea to Fourth-Year Veterinary Students
Authors: Gordon Stuart J. G. , Bolwell Charlotte F. , Raney Jessica L. , Zepke Nick
Published: January 2022

Problem-solving abilities, creative and critical thinking, communication skills, and teamwork are now recognized as fundamental determinants of professional success, especially in vocational professions, such as veterinary science. Tertiary education is now obliged to provide opportunities for students to become proficient in these qualities. With this in mind, the principal author, an equine science senior lecturer, attempted to increase student engagement by developing a new active learning, student-centered one-hour teaching episode on ‘equine diarrhea’, to replace the traditional didactic lecture format. The aim of the study, therefore, was to share the principal author’s journey in the development and implementation of this active learning episode and to explain why it represents a simple but effective method of promoting student engagement. In addition, the adaptation of this method into an online teaching and learning format is briefly discussed. The effectiveness of this active learning method is also explored by comparing it with the traditional didactic method of delivery. Students in the active learning class reported that the learning activity had enhanced their skills in clinical reasoning, problem-solving, and communication. They also described themselves as active participants in the learning process. Students in the traditional didactic class reported that they were satisfied with the time allocated to the lecture, felt that the lecture was well organized and managed, and subsequently felt adequately prepared to answer conventional examination questions relating to equine diarrhea. Other issues, such as student resistance to changes in teaching formats, the effectiveness of group work or teamwork, levels of student confidence within an active learning environment, and the importance of high-quality facilitation during active learning activities, are also discussed in this paper. Although the active learning method described is not novel, the authors hope that fellow educators, across any tertiary discipline, might find that the method described represents a quick and simple method of transforming a single didactic lecture into an enjoyable and engaging learning activity.
Conceptualising Creativity and Innovation in the Role of Primary Sector Headteachers
Authors: Beresford-Dey Marie , Ingram Richard , Lakin Liz
Published: January 2022

Schools and policy makers face common challenges driven by external affairs such as economic uncertainty, globalisation, and advances in technology. Rapidly changing educational, societal, and political systems require school leaders to adopt creativity and innovation (Cr&Inn) as an integral feature of their leadership. To understand what Cr&Inn means to those in primary sector headteacher positions, this conceptual paper explores the following questions: How is Cr&Inn defined regarding headteachers within the primary sector? What are the key characteristics of Cr&Inn regarding primary sector headteachers? We incorporate a systematic literature review along with definitions from primary sector headteachers drawn from interview data. Whilst Cr&Inn remain conceptually complex, we provide clarity by proposing a succinct definition and recognising the key characteristics involved which in turn, has the potential to strengthen effective leadership to improve learner outcomes through overcoming unusual challenges. We conclude with a discussion of how misconceptions may negatively influence creative and innovative practices, yet we recognise that Cr&Inn have much to offer those in such dynamic and accountable roles, globally, particularly in times of uncertainty. Through our identification of the characteristics, we provide school leaders and policy makers with a framework to understand what Cr&Inn entails.
Recent Studies Utilizing Artificial Intelligence Techniques for Solving Data Collection, Aggregation and Dissemination Challenges in Wireless Sensor Networks: A Review
Authors: Osamy Walid , Khedr Ahmed M. , Salim Ahmed , AlAli Amal Ibrahim , El-Sawy Ahmed A.
Published: January 2022

The growing importance and widespread adoption of Wireless Sensor Network (WSN) technologies have helped the enhancement of smart environments in numerous sectors such as manufacturing, smart cities, transportation and Internet of Things by providing pervasive real-time applications. In this survey, we analyze the existing research trends with respect to Artificial Intelligence (AI) methods in WSN and the possible use of these methods for WSN enhancement. The main goal of data collection, aggregation and dissemination algorithms is to gather and aggregate data in an energy efficient manner so that network lifetime is enhanced. In this paper, we highlight data collection, aggregation and dissemination challenges in WSN and present a comprehensive discussion on the recent studies that utilized various AI methods to meet specific objectives of WSN, during the span of 2010 to 2021. We compare and contrast different algorithms on the basis of optimization criteria, simulation/real deployment, centralized/distributed kind, mobility and performance parameters. We conclude with possible future research directions. This would guide the reader towards an understanding of up-to-date applications of AI methods with respect to data collection, aggregation and dissemination challenges in WSN. Then, we provide a general evaluation and comparison of different AI methods used in WSNs, which will be a guide for the research community in identifying the mostly adapted methods and the benefits of using various AI methods for solving the challenges related to WSNs. Finally, we conclude the paper stating the open research issues and new possibilities for future studies.
O2O: An Underwater VLC Approach in Baltic and North Sea
Authors: Ali Mohammad Furqan , Ponnimbaduge Perera Tharindu Dilshan , K. Jayakody Dushantha Nalin
Published: January 2022

Recently, underwater visible light communication (UVLC) has become a potential wireless carrier candidate in the acrimonious mingled ocean straits. The combined strait of the North and Baltic ocean is a harsh and strongly turbid aqueous zone that contributes signal fading at a large scale. Due to this, we are proposing a UVLC system within the Baltic–North ocean mingled water under strong turbulence channel conditions. In this study, the Gamma–Gamma distribution is used to model UVLC link under an OOK modulation scheme. Subsequently, the reason for the unavailability of the latest North–Baltic oceanographic data within this bayou, we investigate the BER and outage probability performance of the proposed system within the mingled strait for the whole year during 1996s. Throughout, this work, the performance is obtained individually in both of the oceans and then compared with the heterogeneous state. It is noteworthy that the analytical work has been considered of the following distinct physio-chemical properties and the data provided for each ocean. Additionally, the simulation results are verified the analytical work of the proposed system model.
Small Group Delay Variation and High Efficiency 3.1–10.6 GHz CMOS Power Amplifier for UWB Systems
Authors: Ali Mayar , Hamed Hesham F. A. , Fahmy Ghazal A.
Published: January 2022

A two-stage cascaded power amplifier (PA) employing a proposed Resistor-Capacitor (RC) interstage was provided and simulated. The current-reuse topology is employed at the first stage to lower the power consumption, while the RC interstage helps to enrich the gain flatness and the wideband matching. The shunt peaking topology in a common source configuration is adopted at the second stage to enhance the power gain. The postlayout simulation is performed using the TSMC 65 nm CMOS process operating in a frequency band of 3.1 GHz to 10.6 GHz. The postlayout simulation results indicate that a high flat gain of approximately 22.8 ± 1.2 dB, small group delay variation of ±50 ps, and good input and output matching of less than −10 dB are achieved over the desired working band. Moreover, a saturated output power of 10 dBm and maximum power-added efficiency (PAE) of 29.5% is achieved at 6 GHz. The proposed PA consumes the low power of 15.5 mW from 1.2 V supply voltage.