bluuebunny/crossref_metadata_2025 huggingface dataset:
Rambellisea gigliensis and Rambellisea halocynthiae, gen. et spp. nov. (Lulworthiaceae) from the Marine Tunicate Halocynthia papillosa
Authors: Braconcini Martina , Gorrasi Susanna , Fenice Massimiliano , Barghini Paolo , Pasqualetti Marcella
Published: February 2024

In this study, 15 Lulworthiales strains isolated from the marine tunicate Halocynthia papillosa collected in the central Tyrrhenian Sea were characterized using a polyphasic approach (morpho-physiological, molecular, and phylogenetic analyses). Based on multi-locus phylogenetic inference and morphological characters, a new genus, Rambellisea, and two new species, R. halocynthiae and R. gigliensis (Lulworthiales), were proposed. Multi-locus phylogenetic analyses using the nuclear ribosomal regions of DNA (nrITS1-nr5.8S-nrITS2, nrLSU, and nrSSU) sequence data strongly supported the new taxa. Phylogenetic inference, estimated using Maximum Likelihood and Bayesian Inference, clearly indicates that Rambellisea gen. nov. forms a distinct clade within the order Lulworthiales. Moreover, the two new species were separated into distinct subclades, solidly supported by the analyses. This is the first report of Lulworthiales species isolated from animals.
Time-Course Responses of Apple Leaf Endophytes to the Infection of Gymnosporangium yamadae
Authors: Li Yunfan , Tao Siqi , Liang Yingmei
Published: February 2024

Apple rust, caused by Gymnosporangium yamadae, poses a significant challenge to apple production. Prior studies have underscored the pivotal role played by endophytic microbial communities, intimately linked with the host, in influencing plant diseases and their pathogenic outcomes. The objective of this study is to scrutinize alternations in endophytic microbial communities within apple leaves at different stages of apple rust using high-throughput sequencing technology. The findings revealed a discernible pattern characterized by an initial increase and subsequent decrease in the alpha diversity of microbial communities in diseased leaves. A microbial co-occurrence network analysis revealed that the complexity of the bacterial community in diseased leaves diminished initially and then rebounded during the progression of the disease. Additionally, employing the PICRUSt2 platform, this study provided preliminary insights into the functions of microbial communities at specific disease timepoints. During the spermogonial stage, endophytic bacteria particularly exhibited heightened activity in genetic information processing, metabolism, and environmental information processing pathways. Endophytic fungi also significantly enriched a large number of metabolic pathways during the spermogonial stage and aecial stage, exhibiting abnormally active life activities. These findings establish a foundation for comprehending the role of host endophytes in the interaction between pathogens and hosts. Furthermore, they offer valuable insights for the development and exploitation of plant endophytic resources, thereby contributing to enhanced strategies for managing apple rust.
Characterization of Three Types of Elongases from Different Fungi and Site-Directed Mutagenesis
Authors: Wang Yuxin , Chang Lulu , Zhang Hao , Chen Yong Q. , Chen Wei , Chen Haiqin
Published: February 2024

Fatty acid elongases play crucial roles in synthesizing long-chain polyunsaturated fatty acids. Identifying more efficient elongases is essential for enhancing oleaginous microorganisms to produce high yields of target products. We characterized three elongases that were identified with distinct specificities: McELO from Mucor circinelloides, PrELO from Phytophthora ramorum, and PsELO from Phytophthora sojae. Heterologous expression in Saccharomyces cerevisiae showed that McELO preferentially elongates C16 to C18 fatty acids, PrELO targets Δ6 polyunsaturated fatty acids, and PsELO uses long chain saturated fatty acids as substrates. McELO and PrELO exhibited more homology, potentially enabling fatty acid composition remodeling and enhanced LC-PUFAs production in oleaginous microorganisms. Site-directed mutagenesis of conserved amino acids across elongase types identified residues essential for activity, supported by molecular docking. Alanine substitution of conserved polar residues led to enzyme inactivation, underscoring their importance in the condensation reaction. Our findings offer promising elongase candidates for polyunsaturated fatty acid production, contributing to the bioindustry’s sustainable development.
Greek Public Sector’s Efficient Resource Allocation: Key Findings and Policy Management
Authors: Petropoulos Theofanis , Thalassinos Yannis , Liapis Konstantinos
Published: February 2024

The public sector has limited resources, and how these resources are allocated in expenditures and investments is crucial. Our article focuses on controlling this allocation for the Greek economy from 2000 to 2021, which includes the country’s debt crisis. To do so, we utilized data from national accounts, categorized inputs and outputs, and examined their volatility and stability over time using statistical and mathematical methods. Our analysis revealed that the crisis impacted the size and allocation of public inputs and outputs. While some sectors of the Greek economy displayed stability in financing over time, others were more volatile. Using a mathematical accounting approach contributes to the academic discourse on rational resource allocation in the public sector. Our results validate the tax hypothesis for primary revenues and expenditures and advocate that it is necessary to make targeted recruitments in the sector that is needed each time while keeping the total number constant, which leads to the need to redistribute public sector workers. In the same way, public projects should not only focus on infrastructure projects but should also be spread to new areas related to climate change and the agricultural sector.
Developing Robust Safety Protocols for Radiosurgery within Patient Positioning System Framework
Authors: Saadah Alaa , Medlin Donald , Saud Jad , Menyhárt Levente , Zheng Xiaoran , Husi Géza
Published: February 2024

This paper offers a comprehensive examination of the development and implementation of advanced safety protocols in the Patient Positioning System (PPS) for radiosurgery. In an era where precision and safety are increasingly crucial in medical procedures, particularly radiosurgery, the implementation of sophisticated safety measures in PPS is vital. This research delves into the detailed design of the system, emphasizing the sensor and controller mechanisms employed. A significant focus is placed on comparing single-loop and dual-loop control systems, assessing their impact on the precision, accuracy, and repeatability of the PPS. The study showcases how dual-loop control demonstrates superior performance in these areas, leading to enhanced patient safety and treatment outcomes. Additionally, the paper discusses the integration of these safety protocols within the system’s architecture, underscoring the practical implications of these advanced measures in augmenting patient safety and treatment effectiveness.
Optimal Hybrid Pulse Width Modulation for Three-Phase Inverters in Electric Propulsion Ships
Authors: Roh Chan , Jeon Hyeon-min , Kim Seong-wan , Kim Jong-su , Lee Na-young , Song Sung-woo
Published: February 2024

Global interest in environmentally friendly ships has surged as a result of greenhouse gas reduction policies and the demand for carbon neutrality. Despite growing demand for electric propulsion systems, there is a lack of research and development on crucial components. Efficiency and stability are primarily influenced by the performance of inverters, which are essential for driving propulsion motors. Existing inverter control techniques can be of two types: continuous-PWM (pulse width modulation) methods for harmonic performance enhancement and discontinuous-PWM methods for efficiency improvement by reducing losses. However, there are limitations in that each PWM method exhibits substantial variations in inverter performance based on its operating conditions. To address these challenges, this study proposes the hybrid pulse-width-modulation (HPWM) method for optimal inverter operation. By analyzing the inverter’s operating conditions, the proposed HPWM method adopts various pulse-width-modulation (PWM) strategies based on a modulation index to achieve harmonic improvement and loss reduction. Our focus is on comparing and analyzing diverse PWM techniques under varying modulation indices and frequency conditions to attain the optimal operating conditions. Experimental validation of the proposed method was conducted using a 2.2 kW dynamometer. In comparison with existing PWM methods, the proposed method demonstrated superior performance.
A Comprehensive Analysis of MSE in Estimating Conditional Hazard Functions: A Local Linear, Single Index Approach for MAR Scenarios
Authors: Belguerna Abderrahmane , Daoudi Hamza , Abdelhak Khadidja , Mechab Boubaker , Chikr Elmezouar Zouaoui , Alshahrani Fatimah
Published: February 2024

In unveiling the non-parametric estimation of the conditional hazard function through the local linear method, our study yields key insights into the method’s behavior. We present rigorous analyses demonstrating the mean square convergence of the estimator, subject to specific conditions, within the realm of independent observations with missing data. Furthermore, our contributions extend to the derivation of expressions detailing both bias and variance of the estimator. Emphasizing the practical implications, we underscore the applicability of two distinct models discussed in this paper for single index estimation scenarios. These findings not only enhance our understanding of survival analysis methodologies but also provide practitioners with valuable tools for navigating the complexities of missing data in the estimation of conditional hazard functions. Ultimately, our results affirm the robustness of the local linear method in non-parametrically estimating the conditional hazard function, offering a nuanced perspective on its performance in the challenging context of independent observations with missing data.
The Lattice Boltzmann Method Using Parallel Computation: A Great Potential Solution for Various Complicated Acoustic Problems
Authors: Pranowo  , Setyohadi Djoko Budiyanto , Wijayanta Agung Tri
Published: February 2024

This paper proposes the D2Q5 Lattice Boltzmann method (LBM) method, in two dimensions with five discrete lattice velocities, for simulating linear sound wave propagation in closed rooms. A second-order linear acoustic equation obtained from the LBM method was used as the model equation. Boundary conditions at the domain boundary use the bounce-back scheme. The LBM numerical calculation algorithm in this paper is relatively simpler and easy to implement. Parallelization with the GPU CUDA was implemented to speed up the execution time. The calculation results show that the use of parallel GPU CUDA programming can accelerate the proposed simulation 27.47 times faster than serial CPU programming. The simulation results are validated with analytical solutions for acoustic pulse reflected by the flat and oblique walls, the comparisons show very good concordance, and the D2Q5 LBM has second-order accuracy. In addition, the simulation results in the form of wavefront propagation images in complicated shaped rooms are also compared with experimental photographs, and the comparison also shows excellent concordance. The numerical results of the D2Q5 LBM are promising and also demonstrate the great capability of the D2Q5 LBM for investigating room acoustics in various complexities.
Endovascular Treatment of Traumatic Vascular Injuries in the Head and Neck Region
Authors: Koh Dong Hyun , Choi Ho Cheol , Shin Hwa Seon , Baek Hye Jin , Koh Eun Ha , Park Mi Jung , Choi Dae Seob
Published: February 2024

Background and Objectives: Traumatic vascular injuries of the head and neck pose significant treatment challenges due to the complex anatomy, diverse clinical presentation, and mostly emergent nature. Endovascular treatment increasingly complements traditional surgical approaches. This study aimed to report our 10-year experience in treating traumatic vascular injuries of the head and neck with endovascular therapy and to determine the effectiveness of endovascular treatment. Materials and Methods: A retrospective analysis of 21 patients treated for head and neck vascular injuries between May 2011 and April 2021 was performed. Patients’ medical histories, clinical presentations, imaging findings, treatment materials, and clinical outcomes were reviewed. Treatments included stenting, coil embolization, and other endovascular techniques focused on hemostasis and preservation of the parent vessel. Results: The most common injuries involved the internal maxillary artery branches (n = 11), followed by the common or internal carotid artery (n = 6), vertebral artery (n = 3), and others. Endovascular treatment achieved successful hemostasis in all but one case. In five of six carotid artery injuries and two of three vertebral artery injuries, we achieved successful hemostasis while preserving the parent vessel using covered and bare stents, respectively. Conclusions: Endovascular therapy might be a useful treatment modality for traumatic vascular injuries in the head and neck region, offering efficacy, safety, and a minimally invasive approach.
Management of Male Fertility in Hypogonadal Patients on Testosterone Replacement Therapy
Authors: Fink Julius , Ide Hisamitsu , Horie Shigeo
Published: February 2024

Testosterone is crucial in regulating several body functions in men, including metabolic, sexual, and cardiovascular functions, bone and muscle mass, and mental health. Therefore, optimizing testosterone levels in men is an important step to maintaining a healthy body and mind, especially as we age. However, traditional testosterone replacement therapy has been shown to lead to male infertility, caused by negative feedback in the hypothalamic–pituitary–gonadal (HPG) axis. Recent advances in research have led to the discovery of many new methods of administration, which can have more or less suppressive effects on the HPG axis. Also, the usage of ancillary medications instead of or after testosterone administration might help maintain fertility in hypogonadal patients. The goal of this narrative review is to summarize the newest methods for optimizing fertility parameters in patients undergoing treatment for hypogonadism and to provide the necessary information for healthcare providers to make the right treatment choices.
Impact of Concurrent Use of N95 and Surgical Masks on Peripheral Oxygen Saturation and Heart Rate Frequency—A Prospective Study during the COVID-19 Outbreak
Authors: Alessandri-Bonetti Anna , Sangalli Linda , Gallenzi Patrizia
Published: February 2024

Background and Objectives: The COVID-19 outbreak has necessitated the prolonged use of N95 facemasks in addition to traditional surgical facemasks by healthcare workers. The aim of this study was to investigate the effect of wearing N95 facemasks in addition to surgical facemasks on peripheral oxygen saturation (SpO2) and heart rate (HR) among dental professionals during routine care. Materials and Methods: This prospective study compared SpO2 and HR between dental providers wearing N95 + surgical facemasks vs. wearing a surgical facemask only. SpO2 and HR were recorded using a portable pulse oximeter before wearing the facemask (T0); at 30 min (T1); at 1 h (T2); and at the end of clinical activity (T3). Inter-group and intra-group differences were assessed with independent t tests and repeated measures ANOVA, respectively. Results: A total of 88 participants (57 wearing N95 + surgical facemasks, and 31 wearing a surgical facemask only) completed the study. The two groups did not statistically differ in SpO2 at different timepoints nor showed any intra-group differences. The participants wearing N95 + surgical facemasks exhibited a statistically higher HR at T0 (p = 0.007), T2 (p = 0.010), and T3 (p = 0.014) compared to those wearing a surgical facemask only. A statistically significant decrease was observed in HR between T0 and T3 in those wearing N95 + surgical facemasks (p = 0.012). No intra-group differences were seen in HR over time in those wearing a surgical facemask only. Conclusions: The continuous use of an N95 in addition to a surgical facemask did not show any significant effects in SpO2 during routine care; however, the concurrent use of an N95 and a surgical facemask seemed to be accompanied by a decrease in HR, although the values remained within the normal range.
Studying Conformational Properties of Transmembrane Domain of KCNE3 in a Lipid Bilayer Membrane Using Molecular Dynamics Simulations
Authors: Moura Anna Clara Miranda , Asare Isaac K. , Cruz Mateo Fernandez , Aguado Antonio Javier Franco , Tuck Kaeleigh Dyan , Campbell Conner C. , Scheyer Matthew W. , Obaseki Ikponwmosa , Alston Steve , Kravats Andrea N. , Sanders Charles R. , Lorigan Gary A. , Sahu Indra D.
Published: February 2024

KCNE3 is a single-pass integral membrane protein that regulates numerous voltage-gated potassium channel functions such as KCNQ1. Previous solution NMR studies suggested a moderate degree of curved α-helical structure in the transmembrane domain (TMD) of KCNE3 in lyso-myristoylphosphatidylcholine (LMPC) micelles and isotropic bicelles with the residues T71, S74 and G78 situated along the concave face of the curved helix. During the interaction of KCNE3 and KCNQ1, KCNE3 pushes its transmembrane domain against KCNQ1 to lock the voltage sensor in its depolarized conformation. A cryo-EM study of KCNE3 complexed with KCNQ1 in nanodiscs suggested a deviation of the KCNE3 structure from its independent structure in isotropic bicelles. Despite the biological significance of KCNE3 TMD, the conformational properties of KCNE3 are poorly understood. Here, all atom molecular dynamics (MD) simulations were utilized to investigate the conformational dynamics of the transmembrane domain of KCNE3 in a lipid bilayer containing a mixture of POPC and POPG lipids (3:1). Further, the effect of the interaction impairing mutations (V72A, I76A and F68A) on the conformational properties of the KCNE3 TMD in lipid bilayers was investigated. Our MD simulation results suggest that the KCNE3 TMD adopts a nearly linear α helical structural conformation in POPC-POPG lipid bilayers. Additionally, the results showed no significant change in the nearly linear α-helical conformation of KCNE3 TMD in the presence of interaction impairing mutations within the sampled time frame. The KCNE3 TMD is more stable with lower flexibility in comparison to the N-terminal and C-terminal of KCNE3 in lipid bilayers. The overall conformational flexibility of KCNE3 also varies in the presence of the interaction-impairing mutations. The MD simulation data further suggest that the membrane bilayer width is similar for wild-type KCNE3 and KCNE3 containing mutations. The Z-distance measurement data revealed that the TMD residue site A69 is close to the lipid bilayer center, and residue sites S57 and S82 are close to the surfaces of the lipid bilayer membrane for wild-type KCNE3 and KCNE3 containing interaction-impairing mutations. These results agree with earlier KCNE3 biophysical studies. The results of these MD simulations will provide complementary data to the experimental outcomes of KCNE3 to help understand its conformational dynamic properties in a more native lipid bilayer environment.
Pysanky to Microfluidics: An Innovative Wax-Based Approach to Low Cost, Rapid Prototyping of Microfluidic Devices
Authors: Schneider Philip J. , Christie Liam B. , Eadie Nicholas M. , Siskar Tyler J. , Sukhotskiy Viktor , Koh Domin , Wang Anyang , Oh Kwang W.
Published: February 2024

A wax-based contact printing method to create microfluidic devices is demonstrated. This printing technology demonstrates a new pathway to rapid, cost-effective device prototyping, eliminating the use of expensive micromachining equipment and chemicals. Derived from the traditional Ukrainian Easter egg painting technique called “pysanky” a series of microfluidic devices were created. Pysanky is the use of a heated wax stylus, known as a “kistka”, to create micro-sized, intricate designs on the surface of an egg. The proposed technique involves the modification of an x-y-z actuation translation system with a wax extruder tip in junction with Polydimethysiloxane (PDMS) device fabrication techniques. Initial system optimization was performed considering design parameters such as extruder tip size, contact angle, write speed, substrate temperature, and wax temperature. Channels created ranged from 160 to 900 μm wide and 10 to 150 μm high based upon system operating parameters set by the user. To prove the capabilities of this technology, a series of microfluidic mixers were created via the wax technique as well as through traditional photolithography: a spiral mixer, a rainbow mixer, and a linear serial dilutor. A thermo-fluidic computational fluid dynamic (CFD) model was generated as a means of enabling rational tuning, critical to the optimization of systems in both normal and extreme conditions. A comparison between the computational and experimental models yielded a wax height of 57.98 μm and 57.30 μm, respectively, and cross-sectional areas of 11,568 μm2 and 12,951 μm2, respectively, resulting in an error of 1.18% between the heights and 10.76% between the cross-sectional areas. The device’s performance was then compared using both qualitative and quantitative measures, considering factors such as device performance, channel uniformity, repeatability, and resolution.
S-Gene Target Failure as an Effective Tool for Tracking the Emergence of Dominant SARS-CoV-2 Variants in Switzerland and Liechtenstein, Including Alpha, Delta, and Omicron BA.1, BA.2, and BA.4/BA.5
Authors: Hilti Dominique , Wehrli Faina , Berchtold Sabine , Bigler Susanna , Bodmer Thomas , Seth-Smith Helena M. B. , Roloff Tim , Kohler Philipp , Kahlert Christian R. , Kaiser Laurent , Egli Adrian , Risch Lorenz , Risch Martin , Wohlwend Nadia
Published: February 2024

During the SARS-CoV-2 pandemic, the Dr. Risch medical group employed the multiplex TaqPathTM COVID-19 CE-IVD RT-PCR Kit for large-scale routine diagnostic testing in Switzerland and the principality of Liechtenstein. The TaqPath Kit is a widely used multiplex assay targeting three genes (i.e., ORF1AB, N, S). With emergence of the B.1.1.7 (Alpha) variant, a diagnostic flaw became apparent as the amplification of the S-gene target was absent in these samples due to a deletion (ΔH69/V70) in the Alpha variant genome. This S-gene target failure (SGTF) was the earliest indication of a new variant emerging and was also observed in subsequent variants such as Omicron BA.1 and BA4/BA.5. The Delta variant and Omicron BA.2 did not present with SGTF. From September 2020 to November 2022, we investigated the applicability of the SGTF as a surrogate marker for emerging variants such as B.1.1.7, B.1.617.2 (Delta), and Omicron BA.1, BA.2, and BA.4/BA.5 in samples with cycle threshold (Ct) values < 30. Next to true SGTF-positive and SGTF-negative samples, there were also samples presenting with delayed-type S-gene amplification (higher Ct value for S-gene than ORF1ab gene). Among these, a difference of 3.8 Ct values between the S- and ORF1ab genes was found to best distinguish between “true” SGTF and the cycle threshold variability of the assay. Samples above the cutoff were subsequently termed partial SGTF (pSGTF). Variant confirmation was performed by whole-genome sequencing (Oxford Nanopore Technology, Oxford, UK) or mutation-specific PCR (TIB MOLBIOL). In total, 17,724 (7.4%) samples among 240,896 positives were variant-confirmed, resulting in an overall sensitivity and specificity of 93.2% [92.7%, 93.7%] and 99.3% [99.2%, 99.5%], respectively. Sensitivity was increased to 98.2% [97.9% to 98.4%] and specificity lowered to 98.9% [98.6% to 99.1%] when samples with pSGTF were included. Furthermore, weekly logistic growth rates (α) and sigmoid’s midpoint (t0) were calculated based on SGTF data and did not significantly differ from calculations based on comprehensive data from GISAID. The SGTF therefore allowed for a valid real-time estimate for the introduction of all dominant variants in Switzerland and Liechtenstein.
Frequency and Molecular Identification of Cryptosporidium in Adult Prim’Holstein Dairy Cattle Farms in the North of France
Authors: Certad Gabriela , Gantois Nausicaa , Merlin Sophie , Martel Sophie , Even Gaël , Viscogliosi Eric , Audebert Christophe , Chabé Magali
Published: February 2024

Cryptosporidium apicomplexan protozoa are ubiquitous intracellular agents affecting humans and animals. In particular, bovine cryptosporidiosis is recognized as endemic worldwide. However, epidemiological investigations remain limited in France regarding the burden of these parasites in cattle. To improve our understanding of the epidemiology of cryptosporidiosis, the main aim of this study was to determine the frequency and the genetic diversity of Cryptosporidium in adult Prim’Holstein dairy cattle farms in the north of France. Fecal specimens were collected from 1454 non-diarrheic and non-pregnant animals (nulli-, primi-, or multiparous) throughout 20 farms in an area of 110 km around Lille. For Cryptosporidium species identification, nested PCR followed by sequence and phylogenetic analyses were used. The overall frequency of Cryptosporidium spp. in-fection was 30.00% (C.I. 95%: 12.83–54.33) in farms and 0.89% (C.I. 95%: 0.498–1.57) at the individual level. In primi- or multiparous cows, only C. andersoni was found. C. ryanae, C. bovis/xiaoi and C. andersoni were detected in heifers. The phylogenetic tree confirmed that analyzed sequences were grouped with known reference sequences reported in dairy cattle. Further studies on the cumulative prevalence, risks factors and pathogenicity are needed to give a more accurate assessment of the impact of Cryptosporidium infection in dairy cattle in France.
A Comparative Study of Quartz and Potassium Feldspar Flotation Process Using Different Chemical Reagents
Authors: Mohanty Kalyani , Oliva Josep , Alfonso Pura , Sampaio Carlos Hoffmann , Anticoi Hernan
Published: February 2024

An improved method for producing high-purity quartz (Qtz) and potassium feldspar (Kfs) concentrates was developed using various chemical reagents. Froth flotation experiments on a Qtz–Kfs mixture showed that quartz could be selectively floated from Kfs in diluted hydrofluoric acid (HF) using a frother. Similarly, feldspar could be selectively floated from quartz. Recovery rates depended on reagent choice, pH levels, HF modifier dosage, and HF conditioning time. High recoveries and good grades were achieved for both quartz and feldspar at low pH levels. Among the five collectors tested, Brij C20 showed the highest recovery when used in conjunction with HF and a frother alone and a concentrate containing 82.8% quartz was obtained at a 96% recovery rate. Good recoveries were also achieved with collectors like Duomeen C and Flotigam V4343 for floating K-feldspar, with a Kfs concentrate of 99.9% purity at a 94% recovery. Lilaflot OT 55 and Duomeen TDO were less effective. The concentration of HF was 22 kg/t, which implies an important reduction with respect to the earlier research.
Gold and Arsenic in Pyrite and Marcasite: Hydrothermal Experiment and Implications to Natural Ore-Stage Sulfides
Authors: Kovalchuk Elena V. , Tagirov Boris R. , Borisovsky Sergei E. , Nickolsky Maximilian S. , Tyukova Evgeniya E. , Sidorova Nina V. , Komarov Vladimir B. , Mezhueva Anna A. , Prokofiev Vsevolod Yu. , Vikentyev Ilya V.
Published: February 2024

Hydrothermal synthesis experiments were performed in order to quantify the states of Au and As in pyrite and marcasite. The experiments were performed at 350 °C/500 bar and 490 °C/1000 bar (pyrite–pyrrhotite buffer, C(NaCl) = 15 and 35 wt.%). The synthesis products were studied by EPMA, LA-ICP-MS, and EBSD. The EPMA was applied for simultaneous determinations of Au, As, Fe, and S, with a Au detection limit of 45–48 ppm (3σ). The analyses were performed along profiles across zonal grains. The concentrations of As and Au up to 5 wt.% and 8000 ppm, respectively, were determined in pyrite and up to 6 wt.% and 1300 ppm in marcasite. In pyrite, the Au concentration decreases with fluid salinity and temperature increases. Strong positive Au–As correlation and strong negative Au–Fe and As–S correlation were identified in pyrite. Comparison of the correlations with theoretical lines implies Au–As clustering. The cluster stoichiometry is inferred to be [AuAs10]. Most probably, As in pyrite presents in the form of clusters and in the As→S solid solution. Incorporation of Au in As-rich pyrite can be controlled by the reductive deposition mechanism. In marcasite, the concentrations of Au are not correlated with the As content. The [AuAs10] clusters enrich the {210}, {113}, and {111} pyrite faces, where the former exhibits the highest affinity to Au and As. The affinity of {110} and {100} forms to Au and As is lower. Implication of the experimental results to data for natural auriferous pyrite shows that the increase of Au content at C(As) > 0.5–1 wt.% is caused by the incorporation of the Au-As clusters, but not because of the formation of Au→Fe solid solution. Therefore, the concentration of “invisible” gold in pyrite is dictated solely by the hydrothermal fluid chemistry and subsequent ore transformations.
Developing Characteristics of Shale Lamination and Their Impact on Reservoir Properties in the Deep Wufeng–Longmaxi Formation Shale of the Southern Sichuan Basin
Authors: Ma Xiao , Xu Jinqi , Liu Wenhui , Wang Yaohua , Wu Huricha , Tan Jingqiang
Published: February 2024

The deep shale of the Wufeng–Longmaxi Formation in the southern Sichuan Basin has high gas potential. The development characteristics of lamination could significantly impact reservoir property. Samples were investigated using microscopic observation, element analysis, organic petrology analysis, mineralogy analysis, and pore structure analysis to determine the types of laminae and laminasets, clarify the formation conditions of argillaceous lamina and silty lamina as well as their relationships with the sedimentary environment, and explore the influence of laminae on shale reservoir property. Results indicate that the Wufeng Formation shale exhibits weak development of laminae due to bioturbation, while the Longmaxi Formation shale develops continuous, parallel, and plate-like laminae. Compared with light silty lamina-rich shale, dark argillaceous lamina-rich shale usually develops in an anoxic reduction environment, with higher total organic carbon content, porosity, pore volume, specific surface area, and more developed organic matter pores, which can provide greater space for shale gas adsorption and storage. Shales in the middle section of the Longmaxi Formation are characterized by the development of silty-argillaceous interbedded type laminaset, which have good reservoir performance, making them the primary target for deep shale gas exploration and development.
Density Functional Theory Study of Methanol Steam Reforming on Pt3Sn(111) and the Promotion Effect of a Surface Hydroxy Group
Authors: He Ping , Zhu Houyu , Sun Qianyao , Li Ming , Liu Dongyuan , Li Rui , Lu Xiaoqing , Zhao Wen , Chi Yuhua , Ren Hao , Guo Wenyue
Published: February 2024

Methanol steam reforming (MSR) is studied on a Pt3Sn surface using the density functional theory (DFT). An MSR network is mapped out, including several reaction pathways. The main pathway proposed is CH3OH + OH → CH3O → CH2O → CH2O + OH → CH2OOH → CHOOH → COOH → COOH + OH → CO2 + H2O. The adsorption strengths of CH3OH, CH2O, CHOOH, H2O and CO2 are relatively weak, while other intermediates are strongly adsorbed on Pt3Sn(111). H2O decomposition to OH is the rate-determining step on Pt3Sn(111). The promotion effect of the OH group is remarkable on the conversions of CH3OH, CH2O and trans-COOH. In particular, the activation barriers of the O–H bond cleavage (e.g., CH3OH → CH3O and trans-COOH → CO2) decrease substantially by ~1 eV because of the involvement of OH. Compared with the case of MSR on Pt(111), the generation of OH from H2O decomposition is more competitive on Pt3Sn(111), and the presence of abundant OH facilitates the combination of CO with OH to generate COOH, which accounts for the improved CO tolerance of the PtSn alloy over pure Pt.
Circular RNAs, Noncoding RNAs, and N6-methyladenosine Involved in the Development of MAFLD
Authors: Nakashima Moeka , Suga Naoko , Ikeda Yuka , Yoshikawa Sayuri , Matsuda Satoru
Published: February 2024

Noncoding RNAs (ncRNAs), including circular RNAs (circRNAs) and N6-methyladenosine (m6A), have been shown to play a critical role in the development of various diseases including obesity and metabolic disorder-associated fatty liver disease (MAFLD). Obesity is a chronic disease caused by excessive fat accumulation in the body, which has recently become more prevalent and is the foremost risk factor for MAFLD. Causes of obesity may involve the interaction of genetic, behavioral, and social factors. m6A RNA methylation might add a novel inspiration for understanding the development of obesity and MAFLD with post-transcriptional regulation of gene expression. In particular, circRNAs, microRNAs (miRNAs), and m6A might be implicated in the progression of MAFLD. Interestingly, m6A modification can modulate the translation, degradation, and other functions of ncRNAs. miRNAs/circRNAs can also modulate m6A modifications by affecting writers, erasers, and readers. In turn, ncRNAs could modulate the expression of m6A regulators in different ways. However, there is limited evidence on how these ncRNAs and m6A interact to affect the promotion of liver diseases. It seems that m6A can occur in DNA, RNA, and proteins that may be associated with several biological properties. This study provides a mechanistic understanding of the association of m6A modification and ncRNAs with liver diseases, especially for MAFLD. Comprehension of the association between m6A modification and ncRNAs may contribute to the development of treatment tactics for MAFLD.
Concurrent Ingestion of Alkaline Water and L-Glutamine Enhanced Salivary α-Amylase Activity and Testosterone Concentration in Boxing Athletes
Authors: Lu Tung-Lin , He Cheng-Shiun , Suzuki Katsuhiko , Lu Chi-Cheng , Wang Chung-Yuan , Fang Shih-Hua
Published: February 2024

Athletes often take sport supplements to reduce fatigue and immune disturbances during or after training. This study evaluated the acute effects of concurrent ingestion of alkaline water and L-glutamine on the salivary immunity and hormone responses of boxers after training. Twelve male boxing athletes were recruited in this study. During regular training, the participants were randomly divided into three groups and asked to consume 400 mL of alkaline water (Group A), 0.15 g/kg body weight of L-glutamine with 400 mL of water (Group G), and 0.15 g/kg of L-glutamine with 400 mL of alkaline water (Group A+G) at the same time each day for three consecutive weeks. Before and immediately after the training, saliva, heart rates, and the rate of perceived exertion were investigated. The activity of α-amylase and concentrations of lactoferrin, immunoglobulin A (IgA), testosterone, and cortisol in saliva were measured. The results showed that the ratio of α-amylase activity/total protein (TP) significantly increased after training in Group A+G but not in Group A or G, whereas the ratios of lactoferrin/TP and IgA/TP were unaffected in all three groups. The concentrations of salivary testosterone after training increased significantly in Group A+G but not in Group A or G, whereas the salivary cortisol concentrations were unaltered in all groups. In conclusion, concurrent ingestion of 400 mL of alkaline water and 0.15 g/kg of L-glutamine before training enhanced the salivary α-amylase activity and testosterone concentration of boxers, which would be beneficial for post-exercise recovery.
Niosomes: Composition, Formulation Techniques, and Recent Progress as Delivery Systems in Cancer Therapy
Authors: Liga Sergio , Paul Cristina , Moacă Elena-Alina , Péter Francisc
Published: February 2024

Niosomes are vesicular nanocarriers, biodegradable, relatively non-toxic, stable, and inexpensive, that provide an alternative for lipid-solid carriers (e.g., liposomes). Niosomes may resolve issues related to the instability, fast degradation, bioavailability, and insolubility of different drugs or natural compounds. Niosomes can be very efficient potential systems for the specific delivery of anticancer, antioxidant, anti-inflammatory, antimicrobial, and antibacterial molecules. This review aims to present an overview of their composition, the most common formulation techniques, as well as of recent utilizations as delivery systems in cancer therapy.
Nanogallium-poly(L-lactide) Composites with Contact Antibacterial Action
Authors: Kurtjak Mario , Maček Kržmanc Marjeta , Spreitzer Matjaž , Vukomanović Marija
Published: February 2024

In diverse biomedical and other applications of polylactide (PLA), its bacterial contamination and colonization are unwanted. For this reason, this biodegradable polymer is often combined with antibacterial agents or fillers. Here, we present a new solution of this kind. Through the process of simple solvent casting, we developed homogeneous composite films from 28 ± 5 nm oleic-acid-capped gallium nanoparticles (Ga NPs) and poly(L-lactide) and characterized their detailed morphology, crystallinity, aqueous wettability, optical and thermal properties. The addition of Ga NPs decreased the ultraviolet transparency of the films, increased their hydrophobicity, and enhanced the PLA structural ordering during solvent casting. Albeit, above the glass transition, there is an interplay of heterogeneous nucleation and retarded chain mobility through interfacial interactions. The gallium content varied from 0.08 to 2.4 weight %, and films with at least 0.8% Ga inhibited the growth of Pseudomonas aeruginosa PAO1 in contact, while 2.4% Ga enhanced the effect of the films to be bactericidal. This contact action was a result of unwrapping the top film layer under biological conditions and the consequent bacterial contact with the exposed Ga NPs on the surface. All the tested films showed good cytocompatibility with human HaCaT keratinocytes and enabled the adhesion and growth of these skin cells on their surfaces when coated with poly(L-lysine). These properties make the nanogallium-polyl(L-lactide) composite a promising new polymer-based material worthy of further investigation and development for biomedical and pharmaceutical applications.
Influence of Desialylation on the Drug Binding Affinity of Human Alpha-1-Acid Glycoprotein Assessed by Microscale Thermophoresis
Authors: Šeba Tino , Kerep Robert , Weitner Tin , Šoić Dinko , Keser Toma , Lauc Gordan , Gabričević Mario
Published: February 2024

Human serum alpha-1-acid glycoprotein (AAG) is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances. The sialic acid groups that terminate the N-glycan chains of AAG have been reported to change in response to numerous health conditions and may have an impact on the binding of drugs to AAG. In this study, we quantified the binding between native and desialylated AAG and seven drugs from different pharmacotherapeutic groups (carvedilol, diltiazem, dipyridamole, imipramine, lidocaine, propranolol, vinblastine) using microscale thermophoresis (MST). This method was chosen due to its robustness and high sensitivity, allowing precise quantification of molecular interactions based on the thermophoretic movement of fluorescent molecules. Detailed glycan analysis of native and desialylated AAG showed over 98% reduction in sialic acid content for the enzymatically desialylated AAG. The MST results indicate that desialylation generally alters the binding affinity between AAG and drugs, leading to either an increase or decrease in Kd values, probably due to conformational changes of AAG caused by the different sialic acid content. This effect is also reflected in an increased denaturation temperature of desialylated AAG. Our findings indicate that desialylation impacts free drug concentrations differently, depending on the binding affinity of the drug with AAG relative to human serum albumin (HSA). For drugs such as dipyridamole, lidocaine, and carvedilol, which have a higher affinity for AAG, desialylation significantly changes free drug concentrations. In contrast, drugs such as propranolol, imipramine, and vinblastine, which have a strong albumin binding, show only minimal changes. It is noteworthy that the free drug concentration of dipyridamole is particularly sensitive to changes in AAG concentration and glycosylation, with a decrease of up to 15% being observed, underscoring the need for dosage adjustments in personalized medicine.
Evolution of the Phase Singularity of an Orbital Angular Momentum Beam with an Astigmatism Phase
Authors: Liang Chunhao , Zheng Cuiling , Lian Xinru , Chen Qian , Gao Yaru , Liu Jinsong , Cai Yangjian , Zeng Jun
Published: February 2024

In this study, we explore the impact of the astigmatism phase on the evolution of the phase singularity of an orbital angular momentum (OAM) beam propagating through free space. The results demonstrate that the high-order phase singularity dispersed into a cluster of individual unit phase singularities owing to the astigmatism phase. The number of singularities equaled the topological charge of the OAM beam. By adjusting the astigmatism phase, we could manipulate and control the evolution of the phase singularities, including their displacements and rotation angles. These findings offer significant prospects for customizing 3D vortex lines, optical topologies, and applications involving topological charge measurement, information encoding, and transfer.
High-Power Terahertz Photonic Crystal Surface-Emitting Laser with High Beam Quality
Authors: Liu Junhong , Xu Yunfei , Li Rusong , Zhang Jinchuan , Zhuo Ning , Liu Junqi , Wang Lijun , Cheng Fengmin , Liu Shuman , Liu Fengqi , Lu Quanyong , Zhai Shenqiang
Published: February 2024

The photonic crystal surface-emitting laser (PCSEL) has attracted much attention due to the advantages of a small far-field divergence angle and high output power. Here, we report a high-power terahertz (THz) photonic crystal laser with high beam quality through the optimization of the absorption boundary condition and the introduction of the symmetrically distributed electrodes. Single-mode surface emission at 3.4 THz with the maximum peak output power of 50 mW is demonstrated. Meanwhile, a high symmetric far-field pattern with C6 symmetry and a small divergence angle is achieved. In this device, the integration of the stable single-mode operation, high beam quality and high output power is realized, which may have great significance for practical applications.
Reinforcement of Cement Nanocomposites through Optimization of Mixing Ratio between Carbon Nanotube and Polymer Dispersing Agent
Authors: An Seok Hwan , Kim Ki Yun , Lee Jea Uk
Published: February 2024

Carbon nanotubes (CNTs), known for their exceptional mechanical, thermal, and electrical properties, are being explored as cement nanofillers in the construction field. However, due to the limited water dispersion of CNTs, polymer dispersing agents like polycarboxylate ether (PCE) and sulfonated naphthalene formaldehyde (SNF) are essential for uniform dispersion. In a previous study, PCE and SNF, common cement superplasticizers, effectively dispersed CNTs in cement nanocomposites. However, uncertainties remained regarding the extent to which all dispersing agents interacted efficiently with CNTs. Therefore, this research quantitatively assessed CNT interaction with dispersing agents through dispersion and centrifugation. Approximately 37% of PCE and 50% of SNF persisted compared to CNT after centrifugation. The resulting cement nanocomposites, with optimized mixing ratios, exhibited enhanced compressive strength of about 14% for CNT/PCE (78.13 MPa) and 12.3% for CNT/SNF (76.97 MPa) compared to plain cement (68.52 MPa). XRD results linked strength reinforcement to increased cement hydrate from optimized CNT dispersion. FE-SEM analysis revealed that CNTs were positioned within the pores of the cement. These optimized cement nanocomposites hold promise for improved safety in the construction industry.
Exploring Functional Polymers in the Synthesis of Luminescent ZnO Quantum Dots for the Detection of Cr6+, Fe2+, and Cu2+
Authors: San José Leire , Yuriychuk Nastasiya , García Olga , López-González Mar , Quijada-Garrido Isabel
Published: February 2024

The main aim of this work is to demonstrate that well-defined methacrylate-based copolymers with oligoethylene glycol side chains and functional groups such as thiol and glycidyl, obtained by photo-initiated reversible addition-fragmentation chain transfer (RAFT) in ethanol, are highly suitable as templates in the synthesis and protection of ZnO quantum dots (ZnO QDs) with remarkable photoluminescent properties. While the affinity of thiol groups to metallic surfaces is well established, their interaction with metal oxides has received less scrutiny. Furthermore, under basic conditions, glycidyl groups could react with hydroxyl groups on the surface of ZnO, representing another strategy for hybrid synthesis. The size and crystalline morphology of the resulting hybrids were assessed using DLS, TEM, and XRD, indicating that both polymers, even with a low proportion of functional groups (5% mol) are appropriate as templates and ligands for ZnO QDs synthesis. Notably, thiol-containing polymers yield hybrids with ZnO featuring excellent quantum yield (up to 52%), while polymers with glycidyl groups require combination with the organosilane aminopropyl triethoxysilane (APTES) to achieve optimal results. In both cases, these hybrids exhibited robust stability in both ethanol and aqueous environments. Beyond fundamental research, due to the remarkable photoluminescent properties and affordability, these hybrid ZnO QDs are expected to have potential applications in biotechnology and green science; in particular, in this study, we examined their use in the detection of environmental contaminants like Fe2+, Cr6+, and Cu2+. Specifically, the limit of detection achieved at 1.13 µM for the highly toxic Cr6+ underscores the significant sensing capabilities of the hybrids.
Polybenzoxazine-Based Nitrogen-Containing Porous Carbon and Their Composites with NiCo Bimetallic Oxides for Supercapacitor Applications
Authors: Periyasamy Thirukumaran , Asrafali Shakila Parveen , Kim Seong-Cheol , Kumar Deivasigamani Ranjith , Lee Jaewoong
Published: February 2024

Supercapacitors (SCs) are considered as emerging energy storage devices that bridge the gap between electrolytic capacitors and rechargeable batteries. However, due to their low energy density, their real-time usage is restricted. Hence, to enhance the energy density of SCs, we prepared hetero-atom-doped carbon along with bimetallic oxides at different calcination temperatures, viz., HC/NiCo@600, HC/NiCo@700, HC/NiCo@800 and HC/NiCo@900. The material produced at 800 °C (HC/NiCo@800) exhibits a hierarchical 3D flower-like morphology. The electrochemical measurement of the prepared materials was performed in a three-electrode system showing an enhanced specific capacitance for HC/NiCo@600 (Cs = 1515 F g−1) in 1 M KOH, at a current density of 1 A g−1, among others. An asymmetric SC device was also fabricated using HC/NiCo@800 as anode and HC as cathode (HC/NiCo@600//HC). The fabricated device had the ability to operate at a high voltage window (~1.6 V), exhibiting a specific capacitance of 142 F g−1 at a current density of 1 A g−1; power density of 743.11 W kg−1 and energy density of 49.93 Wh kg−1. Altogether, a simple strategy of hetero-atom doping and bimetallic inclusion into the carbon framework enhances the energy density of SCs.