bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and evaluation of the feasibility, validity, and reliability of a screening tool for determining distress and supportive care needs of adolescents and young adults with cancer in Japan
Authors: Hirayama Takatoshi , Fujimori Maiko , Yanai Yuko , Ishiki Hiroto , Shindo Akie , Tanaka Moeko , Kobayashi Tomomi , Kojima Rebekah , Satomi Eriko
Published: July 2022

Objectives

This study aims to (i) develop a screening tool for determining distress and supportive care needs of adolescent and young adult cancer patients (AYAs) based on the NCCN's Distress Thermometer and Problem List (DTPL), (ii) evaluate its feasibility, discriminant validity, and test–retest reliability in clinical settings, and (iii) report prevalence of distress and unmet needs.

Method

In the development phase, after translation of the Japanese version of the DTPL (DTPL-J) from English into Japanese and back translation, cognitive debriefing was performed. Items in the problem list were modified to better reflect AYAs’ concerns after interviews. The modified items were reviewed and accepted unanimously by healthcare professionals. In the feasibility phase, the DTPL-J for AYAs was used in a clinical setting for 3 months. Descriptive statistics of participants’ demographics, selected items, and DT scores were calculated to report prevalence of distress and unmet needs. Response and referral rates to experts were assessed to evaluate feasibility. Some items were compared with patient demographics to assess discriminant validity. Among the patients who responded at least twice, correlations between two consecutive screenings were assessed to evaluate test–retest reliability.

Results

The DTPL-J consisted of 49 items in five categories. Of 251 patients, 232 (92.4%) were provided the DTPL-J and 230 (91.6%) responded. Based on the DT cutoff of ≥4, 69 of 230 patients (30%) had high distress. Anxiety (n = 85, 36.6%) was the most commonly selected item. Primary nurses referred 45 (21.7%) patients to an attending physician or another expert. Referral rates after DTPL-J use were higher than rates before use, but the difference was not statistically significant (p = 0.06). The items compared were consistent with their social background. A positive correlation was observed between two responses for some items.

Significance of results

The feasibility, discriminant validity, and test–retest reliability of the tool were suggested.
How Vivid Is Your Mental Imagery?
Authors: Jankowska Dorota M. , Karwowski Maciej
Published: July 2022

Abstract: Across five studies (total N > 3,600), we report the psychometric properties of the Polish version of the Vividness of Visual Imagery Questionnaire (VVIQ-2PL). Confirmatory factor analysis confirmed a unidimensional structure of this instrument; measurement invariance concerning participants’ gender was established as well. The VVIQ-2PL showed excellent test-retest reliability, high internal consistency, and adequate construct validity. As predicted, art students scored significantly higher in visual mental imagery than the non-artist group. We discuss these findings alongside future research directions and possible modifications of VVIQ-2PL.
Conjunctival Lymphoma
Authors: McGrath Lindsay A. , Ryan David A. , Warrier Sunil K. , Coupland Sarah E. , Glasson William J.
Published: July 2022

Lymphoma of the conjunctiva is an ocular malignancy derived from clonal proliferation of lymphocytes. The majority of conjunctival lymphoma is extranodal marginal zone B-Cell lymphoma (EMZL), however diffuse large B-cell (DLBCL), follicular (FL), mantle cell (MCL) and T- cell subtypes are also seen. Clinical manifestations are non-specific, but include unilateral or bilateral painless salmon-pink conjunctival lesions. Approaches to treatment have centered around local immunomodulation, often with Interferon-α2b or Rituximab (anti-CD20 monoclonal antibody) with or without radiation. Although conjunctival lymphoma is generally considered an indolent disease, recent advances in next-generation sequencing have improved clinicians’ ability to predict future recurrence or systemic disease through assessment of cytogenic and molecular features. In this paper, we review the classification, clinical features, diagnostic techniques, and emerging strategies for management and prognostication of conjunctival lymphomas.
Maternal and perinatal obesity induce bronchial obstruction and pulmonary hypertension via IL-6-FoxO1-axis in later life
Authors: Selle Jaco , Dinger Katharina , Jentgen Vanessa , Zanetti Daniela , Will Johannes , Georgomanolis Theodoros , Vohlen Christina , Wilke Rebecca , Kojonazarov Baktybek , Klymenko Oleksiy , Mohr Jasmine , v. Koningsbruggen-Rietschel Silke , Rhodes Christopher J. , Ulrich Anna , Hirani Dharmesh , Nestler Tim , Odenthal Margarete , Mahabir Esther , Nayakanti Sreenath , Dabral Swati , Wunderlich Thomas , Priest James , Seeger Werner , Dötsch Jörg , Pullamsetti Soni S. , Alejandre Alcazar Miguel A.
Published: July 2022

Obesity is a pre-disposing condition for chronic obstructive pulmonary disease, asthma, and pulmonary arterial hypertension. Accumulating evidence suggests that metabolic influences during development can determine chronic lung diseases (CLD). We demonstrate that maternal obesity causes early metabolic disorder in the offspring. Here, interleukin-6 induced bronchial and microvascular smooth muscle cell (SMC) hyperproliferation and increased airway and pulmonary vascular resistance. The key anti-proliferative transcription factor FoxO1 was inactivated via nuclear exclusion. These findings were confirmed using primary SMC treated with interleukin-6 and pharmacological FoxO1 inhibition as well as genetic FoxO1 ablation and constitutive activation. In vivo, we reproduced the structural and functional alterations in offspring of obese dams via the SMC-specific ablation of FoxO1. The reconstitution of FoxO1 using IL-6-deficient mice and pharmacological treatment did not protect against metabolic disorder but prevented SMC hyperproliferation. In human observational studies, childhood obesity was associated with reduced forced expiratory volume in 1 s/forced vital capacity ratio Z-score (used as proxy for lung function) and asthma. We conclude that the interleukin-6-FoxO1 pathway in SMC is a molecular mechanism by which perinatal obesity programs the bronchial and vascular structure and function, thereby driving CLD development. Thus, FoxO1 reconstitution provides a potential therapeutic option for preventing this metabolic programming of CLD.
Plastic pollution fosters more microbial growth in lakes than natural organic matter
Authors: Sheridan Eleanor A. , Fonvielle Jérémy A. , Cottingham Samuel , Zhang Yi , Dittmar Thorsten , Aldridge David C. , Tanentzap Andrew J.
Published: July 2022

Plastic debris widely pollutes freshwaters. Abiotic and biotic degradation of plastics releases carbon-based substrates that are available for heterotrophic growth, but little is known about how these novel organic compounds influence microbial metabolism. Here we found leachate from plastic shopping bags was chemically distinct and more bioavailable than natural organic matter from 29 Scandinavian lakes. Consequently, plastic leachate increased bacterial biomass acquisition by 2.29-times when added at an environmentally-relevant concentration to lake surface waters. These results were not solely attributable to the amount of dissolved organic carbon provided by the leachate. Bacterial growth was 1.72-times more efficient with plastic leachate because the added carbon was more accessible than natural organic matter. These effects varied with both the availability of alternate, especially labile, carbon sources and bacterial diversity. Together, our results suggest that plastic pollution may stimulate aquatic food webs and highlight where pollution mitigation strategies could be most effective.
Nonvolatile ferroelectric domain wall memory integrated on silicon
Authors: Sun Haoying , Wang Jierong , Wang Yushu , Guo Changqing , Gu Jiahui , Mao Wei , Yang Jiangfeng , Liu Yuwei , Zhang Tingting , Gao Tianyi , Fu Hanyu , Zhang Tingjun , Hao Yufeng , Gu Zhengbin , Wang Peng , Huang Houbing , Nie Yuefeng
Published: July 2022

Ferroelectric domain wall memories have been proposed as a promising candidate for nonvolatile memories, given their intriguing advantages including low energy consumption and high-density integration. Perovskite oxides possess superior ferroelectric prosperities but perovskite-based domain wall memory integrated on silicon has rarely been reported due to the technical challenges in the sample preparation. Here, we demonstrate a domain wall memory prototype utilizing freestanding BaTiO3 membranes transferred onto silicon. While as-grown BaTiO3 films on (001) SrTiO3 substrate are purely c-axis polarized, we find they exhibit distinct in-plane multidomain structures after released from the substrate and integrated onto silicon due to the collective effects from depolarizing field and strain relaxation. Based on the strong in-plane ferroelectricity, conductive domain walls with reading currents up to nanoampere are observed and can be both created and erased artificially, highlighting the great potential of the integration of perovskite oxides with silicon for ferroelectric domain wall memories.
Lactational delivery of Triclosan promotes non-alcoholic fatty liver disease in newborn mice
Authors: Weber André A. , Yang Xiaojing , Mennillo Elvira , Ding Jeffrey , Watrous Jeramie D. , Jain Mohit , Chen Shujuan , Karin Michael , Tukey Robert H.
Published: July 2022

Here we show that Triclosan (TCS), a high-volume antimicrobial additive that has been detected in human breastmilk, can be efficiently transferred by lactation to newborn mice, causing significant fatty liver (FL) during the suckling period. These findings are relevant since pediatric non-alcoholic fatty liver disease (NAFLD) is escalating in the United States, with a limited mechanistic understanding. Lactational delivery stimulated hepatosteatosis, triglyceride accumulation, endoplasmic reticulum (ER) stress, signs of inflammation, and liver fibrosis. De novo lipogenesis (DNL) induced by lactational TCS exposure is shown to be mediated in a PERK-eIF2α-ATF4-PPARα cascade. The administration of obeticholic acid (OCA), a potent FXR agonist, as well as activation of intestinal mucosal-regenerative gp130 signaling, led to reduced liver ATF4 expression, PPARα signaling, and DNL when neonates were exposed to TCS. It is yet to be investigated but mother to child transmission of TCS or similar toxicants may underlie the recent increases in pediatric NAFLD.
Decoding kinase-adverse event associations for small molecule kinase inhibitors
Authors: Gong Xiajing , Hu Meng , Liu Jinzhong , Kim Geoffrey , Xu James , McKee Amy , Palmby Todd , de Claro R. Angelo , Zhao Liang
Published: July 2022

Small molecule kinase inhibitors (SMKIs) are being approved at a fast pace under expedited programs for anticancer treatment. In this study, we construct a multi-domain dataset from a total of 4638 patients in the registrational trials of 16 FDA-approved SMKIs and employ a machine-learning model to examine the relationships between kinase targets and adverse events (AEs). Internal and external (datasets from two independent SMKIs) validations have been conducted to verify the usefulness of the established model. We systematically evaluate the potential associations between 442 kinases with 2145 AEs and made publicly accessible an interactive web application “Identification of Kinase-Specific Signal” (https://gongj.shinyapps.io/ml4ki). The developed model (1) provides a platform for experimentalists to identify and verify undiscovered KI-AE pairs, (2) serves as a precision-medicine tool to mitigate individual patient safety risks by forecasting clinical safety signals and (3) can function as a modern drug development tool to screen and compare SMKI target therapies from the safety perspective.
Molecular mechanism of toxin neutralization in the HipBST toxin-antitoxin system of Legionella pneumophila
Authors: Zhen Xiangkai , Wu Yongyu , Ge Jinli , Fu Jiaqi , Ye Le , Lin Niannian , Huang Zhijie , Liu Zihe , Luo Zhao-qing , Qiu Jiazhang , Ouyang Songying
Published: July 2022

Toxin-antitoxin (TA) systems are ubiquitous genetic modules in bacteria and archaea. Here, we perform structural and biochemical characterization of the Legionella pneumophila effector Lpg2370, demonstrating that it is a Ser/Thr kinase. Together with two upstream genes, lpg2370 constitutes the tripartite HipBST TA. Notably, the toxin Lpg2370 (HipTLp) and the antitoxin Lpg2369 (HipSLp) correspond to the C-terminus and N-terminus of HipA from HipBA TA, respectively. By determining crystal structures of autophosphorylated HipTLp, its complex with AMP-PNP, and the structure of HipTLp-HipSLp complex, we identify residues in HipTLp critical for ATP binding and those contributing to its interactions with HipSLp. Structural analysis reveals that HipSLp binding induces a loop-to-helix shift in the P-loop of HipTLp, leading to the blockage of ATP binding and inhibition of the kinase activity. These findings establish the L. pneumophila effector Lpg2370 as the HipBST TA toxin and elucidate the molecular basis for HipT neutralization in HipBST TA.
A device-independent quantum key distribution system for distant users
Authors: Zhang Wei , van Leent Tim , Redeker Kai , Garthoff Robert , Schwonnek René , Fertig Florian , Eppelt Sebastian , Rosenfeld Wenjamin , Scarani Valerio , Lim Charles C.-W. , Weinfurter Harald
Published: July 2022

Device-independent quantum key distribution (DIQKD) enables the generation of secret keys over an untrusted channel using uncharacterized and potentially untrusted devices1–9. The proper and secure functioning of the devices can be certified by a statistical test using a Bell inequality10–12. This test originates from the foundations of quantum physics and also ensures robustness against implementation loopholes13, thereby leaving only the integrity of the users’ locations to be guaranteed by other means. The realization of DIQKD, however, is extremely challenging—mainly because it is difficult to establish high-quality entangled states between two remote locations with high detection efficiency. Here we present an experimental system that enables for DIQKD between two distant users. The experiment is based on the generation and analysis of event-ready entanglement between two independently trapped single rubidium atoms located in buildings 400 metre apart14. By achieving an entanglement fidelity of $$ {\mathcal F} \,\ge 0.892(23)$$

ℱ

≥
0.892

(

23

)

and implementing a DIQKD protocol with random key basis15, we observe a significant violation of a Bell inequality of S = 2.578(75)—above the classical limit of 2—and a quantum bit error rate of only 0.078(9). For the protocol, this results in a secret key rate of 0.07 bits per entanglement generation event in the asymptotic limit, and thus demonstrates the system’s capability to generate secret keys. Our results of secure key exchange with potentially untrusted devices pave the way to the ultimate form of quantum secure communications in future quantum networks.
PI3K drives the de novo synthesis of coenzyme A from vitamin B5
Authors: Dibble Christian C. , Barritt Samuel A. , Perry Grace E. , Lien Evan C. , Geck Renee C. , DuBois-Coyne Sarah E. , Bartee David , Zengeya Thomas T. , Cohen Emily B. , Yuan Min , Hopkins Benjamin D. , Meier Jordan L. , Clohessy John G. , Asara John M. , Cantley Lewis C. , Toker Alex
Published: July 2022

In response to hormones and growth factors, the class I phosphoinositide-3-kinase (PI3K) signalling network functions as a major regulator of metabolism and growth, governing cellular nutrient uptake, energy generation, reducing cofactor production and macromolecule biosynthesis1. Many of the driver mutations in cancer with the highest recurrence, including in receptor tyrosine kinases, Ras, PTEN and PI3K, pathologically activate PI3K signalling2,3. However, our understanding of the core metabolic program controlled by PI3K is almost certainly incomplete. Here, using mass-spectrometry-based metabolomics and isotope tracing, we show that PI3K signalling stimulates the de novo synthesis of one of the most pivotal metabolic cofactors: coenzyme A (CoA). CoA is the major carrier of activated acyl groups in cells4,5 and is synthesized from cysteine, ATP and the essential nutrient vitamin B5 (also known as pantothenate)6,7. We identify pantothenate kinase 2 (PANK2) and PANK4 as substrates of the PI3K effector kinase AKT8. Although PANK2 is known to catalyse the rate-determining first step of CoA synthesis, we find that the minimally characterized but highly conserved PANK49 is a rate-limiting suppressor of CoA synthesis through its metabolite phosphatase activity. Phosphorylation of PANK4 by AKT relieves this suppression. Ultimately, the PI3K–PANK4 axis regulates the abundance of acetyl-CoA and other acyl-CoAs, CoA-dependent processes such as lipid metabolism and proliferation. We propose that these regulatory mechanisms coordinate cellular CoA supplies with the demands of hormone/growth-factor-driven or oncogene-driven metabolism and growth.
Effects of polyethylene oxide particles on the photo-physical properties and stability of FA-rich perovskite solar cells
Authors: Koech Richard K. , Olanrewaju Yusuf A. , Ichwani Reisya , Kigozi Moses , Oyewole Deborah O. , Oyelade Omolara V. , Sanni Dahiru M. , Adeniji Sharafadeen A. , Colin-Ulloa Erika , Titova Lyubov V. , Martin Julia L. , Grimm Ronald L. , Bello Abdulhakeem , Oyewole Oluwaseun K. , Ntsoenzok Esidor , Soboyejo Winston O.
Published: July 2022

In this paper, we use Polyethylene Oxide (PEO) particles to control the morphology of Formamidinium (FA)-rich perovskite films and achieve large grains with improved optoelectronic properties. Consequently, a planar perovskite solar cell (PSC) is fabricated with additions of 5 wt% of PEO, and the highest PCE of 18.03% was obtained. This solar cell is also shown to retain up to 80% of its initial PCE after about 140 h of storage under the ambient conditions (average relative humidity of 62.5 ± 3.25%) in an unencapsulated state. Furthermore, the steady-state PCE of the PEO-modified PSC device remained stable for long (over 2500 s) under continuous illumination. This addition of PEO particles is shown to enable the tuning of the optoelectronic properties of perovskite films, improvements in the overall photophysical properties of PSCs, and an increase in resistance to the degradation of PSCs.
Long lasting anxiety following early life stress is dependent on glucocorticoid signaling in zebrafish
Authors: Chin Jacqueline S. R. , Phan Tram-Anh N. , Albert Lydia T. , Keene Alex C. , Duboué Erik R.
Published: July 2022

Chronic adversity in early childhood is associated with increased anxiety and a propensity for substance abuse later in adulthood, yet the effects of early life stress (ELS) on brain development remain poorly understood. The zebrafish, Danio rerio, is a powerful model for studying neurodevelopment and stress. Here, we describe a zebrafish model of ELS and identify a role for glucocorticoid signaling during a critical window in development that leads to long-term changes in brain function. Larval fish subjected to chronic stress in early development exhibited increased anxiety-like behavior and elevated glucocorticoid levels later in life. Increased stress-like behavior was only observed when fish were subjected to ELS within a precise time window in early development, revealing a temporal critical window of sensitivity. Moreover, enhanced anxiety-like behavior only emerges after two months post-ELS, revealing a developmentally specified delay in the effects of ELS. ELS leads to increased levels of baseline cortisol, and resulted in a dysregulation of cortisol receptors’ mRNA expression, suggesting long-term effects on cortisol signaling. Together, these findings reveal a ‘critical window’ for ELS to affect developmental reprogramming of the glucocorticoid receptor pathway, resulting in chronic elevated stress.
Characterization of plant growth-promoting rhizobacteria (PGPR) in Persian walnut associated with drought stress tolerance
Authors: Lotfi Naser , Soleimani Ali , Çakmakçı Ramazan , Vahdati Kourosh , Mohammadi Parisa
Published: July 2022

There is a lack of information on the rhizosphere of nut-bearing trees where microbial populations can benefit roots and tree growth. The current research aimed at discovering plant growth-promoting rhizobacteria (PGPR) in the rhizosphere of soil samples from around the root zone of six walnut trees, each of which was considered as a genotype, i.e. ‘TT1’, ‘TT2’, ‘SS2’, ‘ZM1’, ‘Chandler’ and ‘Haward’. The trees grew in different arid and semiarid regions of Iran and Turkey. The strains were isolated and identified based on different morphological and biochemical markers. Drought-stress tolerance was assessed in the case of each isolate through their transfer to culture medium, containing polyethylene glycol (PEG6000) at 0 and 373.80 g L−1. Resilient strains were analyzed for measuring their ability to produce siderophore, hydrogen cyanide (HCN), Indole-3-acetic acid (IAA) and Gibberellic acid (GA3). In sum, 211 isolates were identified, of which a large number belonged to the Bacillus genus and, specifically, 78% of the strains were able to grow under drought stress conditions. The genus Arthrobacter was only detected in the rhizosphere of ‘ZM1’, ‘Haward’ and ‘TT1’ genotypes. In 4% of the strains, IAA production exceeded 53 mg L−1, while a high level of phosphorus solubility was verified in 6% of the strains. No strain was found to have the capability of producing HCN. The strains were screened for drought-tolerance, which resulted in the discovery of two promising strains, i.e. ZM39 and Cha43. Based on molecular identification through amplification and sequencing of the 16S rDNA gene, these two strains seemed to belong to Bacillus velezensis and Bacillus amyloliquefaciens, respectively. The discovery of new PGPR strains could probably assist walnut trees in improving their mechanisms of adaptation to drought stress.
Vasoactive inotropic score as a predictor of long-term mortality in patients after off-pump coronary artery bypass grafting
Authors: Kwon Ji-Hye , Yoo Seung Yeon , Kim Seonwoo , Won Hojeong , Kim Wooksung , Her Sukyoung , Bang Yu Jeong , Park Jungchan , Lee Jong-Hwan , Cho Hyun Sung , Min Jeong-Jin
Published: July 2022

Increased vasoactive-inotropic score (VIS) is a reliable predictor of mortality and morbidity after cardiac surgery. Here, we retrospectively evaluated the association between VIS and adverse outcomes in adult patients after off-pump coronary artery bypass grafting (OPCAB). We included 2149 patients who underwent OPCAB. The maximal VIS was calculated for the initial 48 postoperative hours using standard formulae. The primary outcome was 1-year death. The composite adverse outcome was death, resuscitation or mechanical support, myocardial infarction, revascularization, new-onset atrial fibrillation, infection requiring antibacterial therapy, acute kidney injury, and stroke. Path-analysis was conducted using lactate and prognostic nutritional index (PNI). VIS was associated with 1-year death (odds ratio [OR] 1.07 [1.04–1.10], p < 0.001) and 1-year composite outcome (OR 1.02 [1.0–1.03], p = 0.008). In path-analysis, high VIS showed a direct effect on the increased risk of 1-year death and composite outcome. In the pathway using lactate as a mediating variable, VIS showed an indirect effect on the composite outcome but no significant effect on death. Low PNI directly affected the increased risk of 1-year death and composite outcome, and had an indirect effect on both outcomes, even when VIS was used as a mediating variable. In patients undergoing OPCAB, high VIS independently predicted morbidity and 1-year death. Patients with increased lactate levels following high VIS had an increased risk of postoperative complications, although not necessarily resulting in death. However, patients with poor preoperative nutritional status had an increased risk of unfavourable outcomes, including death, implying the importance of preoperative nutritional support.
Chromatography columns packed with thermoresponsive-cationic-polymer-modified beads for therapeutic drug monitoring
Authors: Nagase Kenichi , Takagi Hikaru , Nakada Hideo , Ishikawa Haruki , Nagata Yoshiko , Aomori Tohru , Kanazawa Hideko
Published: July 2022

Therapeutic drug monitoring, which is used to determine appropriate drug doses, is critical in pharmacological therapy. In this study, we developed thermoresponsive chromatography columns with various cationic properties for effective therapeutic drug monitoring. Thermoresponsive cationic copolymer poly(N-isopropylacrylamide-co–n-butyl methacrylate-co–N,N-dimethylaminopropyl acrylamide) (P(NIPAAm-co-BMA-co-DMAPAAm))-modified silica beads, which were used as the chromatographic stationary phase, were prepared by modifying the radical initiator of the silica beads, followed by radical polymerization. Characterization of the prepared silica beads demonstrated that thermoresponsive polymers with various cationic properties successfully modified the beads. The elution behavior of several steroids in the prepared bead-packed columns at various temperatures indicated that the optimal column operating temperature was 30 °C. Appropriate measurement conditions for 13 drugs were investigated by varying the cationic properties of the columns and the pH of the mobile phase. Drug concentrations in serum samples were determined using the developed columns and mobile phases with a suitable pH. Voriconazole concentrations in human serum samples were determined using the developed columns with all-aqueous mobile phases. We anticipate that the developed chromatography columns can be used for therapeutic drug monitoring because drug concentrations can be measured using all-aqueous mobile phases that are suitable in clinical settings.
Identification of candidate enhancers controlling the transcriptome during the formation of interphalangeal joints
Authors: Nowosad Karol , Brouwer Rutger W. W. , Odrzywolski Adrian , Korporaal Anne L. , Gielniewski Bartłomiej , Wojtaś Bartosz , van IJcken Wilfred F. J. , Grosveld Frank , Huylebroeck Danny , Tylzanowski Przemko
Published: July 2022

The formation of the synovial joint begins with the visible emergence of a stripe of densely packed mesenchymal cells located between distal ends of the developing skeletal anlagen called the interzone. Recently the transcriptome of the early synovial joint was reported. Knowledge about enhancers would complement these data and lead to a better understanding of the control of gene transcription at the onset of joint development. Using ChIP-sequencing we have mapped the H3-signatures H3K27ac and H3K4me1 to locate regulatory elements specific for the interzone and adjacent phalange, respectively. This one-stage atlas of candidate enhancers (CEs) was used to map the association between these respective joint tissue specific CEs and biological processes. Subsequently, integrative analysis of transcriptomic data and CEs identified new putative regulatory elements of genes expressed in interzone (e.g.,GDF5,BMP2andDACT2) and phalange (e.g.,MATN1,HAPLN1andSNAI1). We also linked such CEs to genes known as crucial in synovial joint hypermobility and osteoarthritis, as well as phalange malformations. These analyses show that the CE atlas can serve as resource for identifying, and as starting point for experimentally validating, putative disease-causing genomic regulatory regions in patients with synovial joint dysfunctions and/or phalange disorders, and enhancer-controlled synovial joint and phalange formation.
Label-free, real-time detection of perineural invasion and cancer margins in a murine model of head and neck cancer surgery
Authors: Tam Kenric , Alhiyari Yazeed , Huang Shan , Han Albert , Stafsudd Oscar , Shori Ramesh , John Maie St.
Published: July 2022

Surgical management of head and neck cancer requires a careful balance between complete resection of malignancy and preservation of function. Surgeons must also determine whether to resect important cranial nerves that harbor perineural invasion (PNI), as sacrificing nerves can result in significant morbidity including facial paralysis. Our group has previously reported that Dynamic Optical Contrast Imaging (DOCI), a novel non-invasive imaging system, can determine margins between malignant and healthy tissues. Herein, we use an in vivo murine model to demonstrate that DOCI can accurately identify cancer margins and perineural invasion, concordant with companion histology. Eight C3H/HeJ male mice were injected subcutaneously into the bilateral flanks with SCCVIISF, a murine head and neck cancer cell line. DOCI imaging was performed prior to resection to determine margins. Both tumor and margins were sent for histologic sectioning. After validating that DOCI can delineate HNSCC margins, we investigated whether DOCI can identify PNI. In six C3H/HeJ male mice, the left sciatic nerve was injected with PBS and the right with SCCVIISF. After DOCI imaging, the sciatic nerves were harvested for histologic analysis. All DOCI images were acquired intraoperatively and in real-time (10 s per channel), with an operatively relevant wide field of view. DOCI values distinguishing cancer from adjacent healthy tissue types were statistically significant (P < 0.05). DOCI imaging was also able to detect perineural invasion with 100% accuracy compared to control (P < 0.05). DOCI allows for intraoperative, real-time visualization of malignant and healthy tissue margins and perineural invasion to help guide tumor resection.
Dissipation behavior and dietary exposure risk of pesticides in Brussels sprout evaluated using LC–MS/MS
Authors: An Dai , Ko Rakdo , Kim Jinchan , Kang Seokhyun , Lee Kwanghun , Lee Jiho
Published: July 2022

In this study, the dissipation behavior and dietary exposure risk of eight pesticides in Brussels sprout were evaluated under greenhouse conditions. Brussels sprout samples were collected 0, 7, 14, and 21 days after the last pesticide treatment. Ultra-high performance liquid chromatography with tandem mass spectrometry was used for sample analysis. Recovery rates at different concentrations of pesticides (0.01 and 0.1 mg/kg) were in the range of 70.2–104.5%, and the relative standard deviations were ≤ 10.6%. The pesticide residues in Brussels sprouts were determined for each treatment. For acephate, etofenprox, imidacloprid, indoxacarb, alpha-cypermethrin, zeta-cypermethrin, fludioxonil, and oxytetracycline, the half-lives were, respectively, 11.3, 9.8, 11.3, 15.8, 10.6, 13, 9.1, and 8.2 d and the dietary intake rates were, respectively, 2.90%, 0.81%, 0.7%, 1.19%, 0.06%, 0.24%, 0.05%, and 0.36% of the acceptable daily intake. The findings of this study provide important insights into the establishment of maximum residue limits in the Republic of Korea and pesticide control measures for Brussels sprout.
Identification and comparison of biological characteristics and pathogenicity of different mating types of V. dahliae isolated from potato and sunflower
Authors: Yan NingNing , Addrah Mandela Elorm , Zhang Yuanyuan , Jia Ruifang , Kang Liru , Zhao Jun , Zhang Jian
Published: July 2022

Potato is one of the most important staple crops in the world. China is one of the leading producers of potatoes, but the industry faces soilborne diseases such as Verticillium wilt. Most potato planting areas in China rotate the crop with sunflower which is also highly susceptible to Verticillium wilt. The comparison of the biological characteristics and pathogenicity of different mating types of Verticillium dahliae isolated from potato and sunflower in the major planting regions in China is of great importance. This is to help unravel the diversity in V. dahliae population and the sudden increase in infected fields. The diseased samples collected were cultured on PDA and the growing colony of pathogen isolated. Molecular techniques using specific primers were used to identify the V. dahliae pathogens and their mating type of the isolates obtained from the diseased sunflower and potato plants as well as their planting materials. The data obtained revealed that the dominant mating type population in sunflower was MAT1-1, whiles that of potato was MAT1-2, but Race 2 was the only race type identified for all the samples. There was a significant presence of MAT1-1 isolates present in potatoes, which is a new trend. Conventional crop rotation farming using sunflower is causing an increasing prevalence of MAT1-1 and mating type shift of isolates in potato in these regions.
A gold nanoparticle-protein G electrochemical affinity biosensor for the detection of SARS-CoV-2 antibodies: a surface modification approach
Authors: Khaniani Yeganeh , Ma Yuhao , Ghadiri Mahdi , Zeng Jie , Wishart David , Babiuk Shawn , Charlton Carmen , Kanji Jamil N. , Chen Jie
Published: July 2022

As COVID-19 waves continue to spread worldwide, demand for a portable, inexpensive and convenient biosensor to determine community immune/infection status is increasing. Here we describe an impedance-based affinity biosensor using Interdigitated Electrode (IDE) arrays to detect antibodies to SARS-CoV-2 in serum. We created the biosensor by functionalizing the IDEs’ surface with abaculaovirus-expressed and purified Spike (S) protein to bind anti-SARS CoV-2antibodies. Gold nanoparticles (GNP) fused to protein G were used to probe for bound antibodies. An ELISA assay using horseradish peroxidase-protein G to probe for bound IgG confirmed that the purified S protein bound a commercial source of anti-SARS-CoV-2 antibodies specifically and bound anti-SARS-CoV-2 antibodies in COVID-19 positive serum. Then we demonstrated that our biosensor could detect anti-SARS-CoV-2 antibodies with 72% sensitivity in 2 h. Using GNP-protein G, the affinity biosensor had increased impedance changes with COVID-19positive serum and minimal or decreased impedance changes with negative serum. This demonstrated that our biosensor could discriminate between COVID-19 positive and negative sera, which were further improved using poly(vinyl alcohol)as a blocking agent.
Macrophage-like cells are still detectable on the retinal surface after posterior vitreous detachment
Authors: Wang Jacob M. , Ong Janice X. , Nesper Peter L. , Fawzi Amani A. , Lavine Jeremy A.
Published: July 2022

The identity of vitreoretinal interface macrophage-like cells (MLCs) remains unknown and potential candidates include retinal microglia, perivascular macrophages, monocyte-derived macrophages, and/or vitreal hyalocytes. Since hyalocytes are detectable on the posterior vitreous surface after vitreous extraction in animals, we imaged patients with and without posterior vitreous detachment (PVD) to determine if hyalocytes are the principal MLC component. We performed repeated foveal-centered 3 × 3 mm OCT-A images from 21 eyes (11 no PVD and 10 PVD eyes). Images were registered, segmented, and averaged. The OCT slab from 0 to 3 microns above the internal limiting membrane was used to detect MLCs. We calculated MLC density and distribution in relation to the superficial vascular plexus for 3 vascular regions—on vessels, perivascular, and non-vascular. MLC density was 1.8-fold greater in the PVD group compared to the no PVD group (P = 0.04). MLCs in eyes with PVD were increased 1.9-fold on-vessel (P = 0.07), 1.9-fold in the perivascular region (P = 0.12), and 2.2-fold in non-vascular areas (P = 0.22). MLC density was not severely reduced after PVD, suggesting that the majority of MLCs are not vitreal hyalocytes. PVD status is an important parameter in future MLC studies.
MWCNT/Ruthenium hydroxide aerogel supercapacitor production and investigation of electrochemical performances
Authors: Korkmaz Satiye , Kariper İshak Afşin , Karaman Ceren , Karaman Onur
Published: July 2022

In this study, the material obtained from the sonication of the double-walled carbon nanotube and ruthenium chloride was produced as an aerogel. Then, symmetrical supercapacitor devices were made using them, and their electrochemical properties were investigated. XRD and FTIR were used in the structural analysis of the aerogel, STEM in surface images, and elemental analyses in EDX. Electrochemical analysis was performed by galvanostat/potentiostat. From the cyclic voltammetry analysis, the highest specific capacitance for MWCNT/Ruthenium hydroxide aerogels was achieved as 423 F/g at 5 mV/s. On the other hand, the corresponding values calculated from the charge–discharge curves were found to be 420.3 F/g and 319.9 F/g at the current densities of 0.5 A/g and 10.0 A/g, respectively. The capacitance retention of as-synthesized aerogel was 96.38% at the end of the 5000 consecutive consecutive cyclic voltammetry cycles.
Remarkable immune and clinical value of novel ferroptosis-related genes in glioma
Authors: Gao Xiaoyan , Zhao Jiazheng , Jia Litao , Zhang Qiushi
Published: July 2022

Ferroptosis is a neoteric model of regulated cell death that shows great potential for the understanding of tumor immunology and as a target for therapy. The present study aimed to identify ferroptosis-related differentially expressed genes (DEGs) in glioma and to explore their value through systematic analysis. Ferroptosis-related DEGs were identified through the Gene Expression Omnibus database in combination with the FerrDb database and analyzed in the Genotype-Tissue Expression database and The Cancer Genome Atlas database. Possible signaling pathways involved were explored by construction of enrichment analysis and protein–protein interaction of these DEGs. Potential regulation of the immune microenvironment, immune checkpoint and chemokine was postulated by immune analysis. A prognosis model for glioma was developed using survival analysis, exhibited by the nomogram and evaluated by the calibration curve. The prognostic value of the model was validated by using an independent cohort. A total of 15 ferroptosis-related DEGs were identified, including 7 down-regulated and 8 up-regulated, with ATP6V1G2, GABARAPL1 and GOT1 as hub genes. The expression of all 3 hub genes was positively correlated with T follicular helper cells and natural killer CD56bright cells. These hub genes were negatively correlated with the macrophage cell type as well as B7H3, PDCD1, LAG3 and CXCL16, CXCR4, CCR5. Low expression of all 3 hub genes was associated with poor prognosis in glioma cases. ATP6V1G2 might be an independent prognostic factor and, as such, a high-precision prognostic model of glioma was constructed. We identified novel ferroptosis-related genes with clinical value in glioma and revealed their possible tumor immune relevance. Furthermore, in glioma, we pinpointed underlying critical elements of the chemokine, immune microenvironment and immune checkpoint, and were able to develop a predictive model of prognosis.
When AIE meets enzymes
Authors: Feng Guangfu , Liao Sijie , Liu Yufeng , Zhang Huaizu , Luo Xingyu , Zhou Xiangming , Fang Jun
Published: July 2022

Applications of AIEgens in biosensing, disease diagnosis, and drug release.
New sulfonated perylene diimide pyrazolate ligands: a simple route toward n-type redox-active hybrid materials
Authors: Monnier Vincent , Odobel Fabrice , Diring Stéphane
Published: July 2022

We report the preparation and physicochemical properties of novel electron accepting sulfonated perylene diimide pyrazolate ligands with low lying LUMO levels.
Molecular copper decomposition ink for printable electronics
Authors: Sheng Aaron , Islam Abdullah , Khuje Saurabh , Yu Jian , Tsang Harvey , Bujanda Andres , Ren Shenqiang
Published: July 2022

Nanostructured metal materials are the frontrunners of numerous electronic advancements.
Dual-channel lanthanide-doped nanoprobe for reliable multi-signal ratiometric detection of H2S in whole blood
Authors: Wei Zheng , Cao Tianqi , Li Luoyuan , Zhu Xingjun , Zhou Jing , Liu Yuxin
Published: July 2022

A multi-signal method eliminates the influence of the wavelength-dependent absorbance of whole-blood samples, ensuring high diagnostic precision in medical practice.
Regioselective ring-opening of epoxides towards Markovnikov alcohols: a metal-free catalytic approach using abnormal N-heterocyclic carbene
Authors: P. Sreejyothi , Sarkar Pallavi , Dutta Supriti , Das Arpan , Pati Swapan K. , Mandal Swadhin K.
Published: July 2022

This work highlights the metal-free Markovnikov ring-opening of epoxides using a nucleophilic abnormal N-heterocyclic carbene (aNHC).
Highly selective molecular sieving of cis- over trans-1,2-dichloroethene isomers
Authors: Liu Xin , Alimi Lukman O. , Khashab Niveen M.
Published: July 2022

An economic and energy-efficient adsorptive molecular sieving approach is demonstrated for the separation of the cis-DCE isomer with a purity of ∼96% using trianglimine macrocycles.
TfOH-promoted synthesis of indoles and benzofurans involving cyclative transposition of vinyl ketone
Authors: Mandal Mou , Balamurugan Rengarajan
Published: July 2022

A metal-free approach to construct indole rings from vinylogous amides derived from o-alkynylanilines involving a cyclization, retro-aza-Michael and amine trapping cascade is reported here. It has been extended to synthesize benzofuran derivatives.
Ferroelectric benzimidazole additive-induced interfacial water confinement for stable 2.2 V supercapacitor electrolytes exposed to air
Authors: Liang Hongyu , Shi Renxing , Zhou Yan , Jiang Wenya , Kang Qin , Zhang Hongyu , Liu Kuanguan , Lian Jiabiao , Bu Yongfeng
Published: July 2022

A novel ferroelectric benzimidazole additive for DESs is reported, exhibiting a 2.2 V voltage in air by confining the water activity at the electrode interface.
Robust NiCoP@FeP derived from Prussian blue analog for efficient overall water splitting
Authors: Pan Yaoyao , Huang Xiaoyu , Huang Yin , Sheng Congyi , Wang Xiuhua
Published: July 2022

NiCoP@FeP hierarchical nanocomposites are successfully constructed for overall water splitting with high stability and low voltage.
Quest for extended coordination networks from highly connected azido-bridged clusters
Authors: Li Shuai , Han Song-De , Zhao Jiong-Peng , Liu Fu-Chen
Published: July 2022

A new type of SBUs composed of high connected azido, particularly the rare μ1,1,1,1-N3 bridged clusters, was extended to coordination networks.
Identification of incommensurability in l-leucine: can lattice instabilities be considered as general phenomena in hydrophobic amino acids?
Authors: Guinet Yannick , Paccou Laurent , Danède Florence , Derollez Patrick , Hédoux Alain
Published: July 2022

The structural organization of leucine molecules which has been focusing a lot of investigations for more than fifty years, must now be considered as a modulated phase at 295 K, the modulation being associated with a continuum of CH3 orientations.
Catalytic activity trends of pyrite transition metal dichalcogenides for oxygen reduction and evolution
Authors: Lee Wooseok , Kim Jongseung , Kim Heejin , Back Seoin
Published: July 2022

In this work, transition metal dichalcogenides are investigated as promising oxygen reduction/evolution reaction catalysts using density functional theory calculations. Multivariate regression models are also developed to predict activity descriptors.
Ion transport mechanism in anhydrous lithium thiocyanate LiSCN part II: frequency dependence and slow jump relaxation
Authors: Joos Markus , Conrad Maurice , Moudrakovski Igor , Terban Maxwell W. , Rad Ashkan , Kaghazchi Payam , Merkle Rotraut , Dinnebier Robert E. , Schleid Thomas , Maier Joachim
Published: July 2022

A frequency dependent conductivity is observed for dry Li(SCN) with local ion hopping at high (ω1), and long-range motion at low frequencies (ω2). The ion transport mechanism of the mobile defect considering cation–anion coordination is discussed.
Critical problems faced in Raman-based energy transport characterization of nanomaterials
Authors: Wang Ridong , Hunter Nicholas , Zobeiri Hamidreza , Xu Shen , Wang Xinwei
Published: July 2022

Conjugated phonon-hot carrier transport, energy-carrier thermal nonequilibrium, optical interference, and ET-Raman are analyzed for advanced Raman-based nanoscale thermal transport measurement.
Two-dimensional carbon materials with an anisotropic Dirac cone: high stability and tunable Fermi velocity
Authors: Liu Shijie , Wang Hui , Ma Fengxian , Du Hui , Liu Bingbing
Published: July 2022

A new 2D Dirac carbon allotrope is proposed, which has unique self-doping properties and a tunable Fermi velocity via the applied strain.
Covalent triazine-based frameworks with cobalt-loading for visible light-driven photocatalytic water oxidation
Authors: Chen Hongmei , Gardner Adrian M. , Lin Guoan , Zhao Wei , Bahri Mounib , Browning Nigel D. , Sprick Reiner Sebastian , Li Xiaobo , Xu Xiaoxiang , Cooper Andrew I.
Published: July 2022

Incorporating Co through the bipyridine coordination sites within CTF results in rapid charge-separation, thus improving the photocatalytic water oxidation performance.
Cr/PCCP-catalysed selective ethylene oligomerization: analysis of various conformations and the hemilabile methoxy group
Authors: Zhong Xuqin , Liu Lin , Guo Xing , Sun Li , Liu Boping , Liu Zhen
Published: July 2022

This work reports a DFT study on the effects of the hemilabile methoxy group in Cr/PCCP and Cr/PCCPOMecatalyzed ethylene tri- and tetramerization. The ensemble of conformers and the key geometrical features of the lowest conformer were discussed.
Development of a series of flurbiprofen and zaltoprofen platinum(iv) complexes with anti-metastasis competence targeting COX-2, PD-L1 and DNA
Authors: Li Zuojie , Li Linming , Zhao Wenhuan , Sun Bin , Liu Zhifang , Liu Min , Han Jun , Wang Zhengping , Li Dacheng , Wang Qingpeng
Published: July 2022

Flurbiprofen platinum(iv) hybrids exhibit effective antiproliferative and anti-metastasis activities targeting COX-2, PD-L1 and DNA, and exert great potential in boosting T-cell immunity.
Cuproussiloxane as two self-assemblies, Cu20O20Si10Me10R10 and Cu24O24Si12Me12R12, with catalytic property
Authors: Jiang Wenjun , Wang Jinjin , Chen Yilin , Chen Zhikang , Li Jiancheng , Jiang Liuyin , Peng Yanbo , Zhu Hongping
Published: July 2022

A cuproussiloxane is prepared and utilized to catalyze the aerobic C–P coupling of terminal alkynes with H-phosphonates to alkynylphosphonates.
Alkali metal influences in aluminyl complexes
Authors: Grams Samuel , Mai Jonathan , Langer Jens , Harder Sjoerd
Published: July 2022

Aluminyl complexes over the full series of alkali metals crystallize as dimers. The alkali metal influences structure, dimerization energies and reactivity.
What stress components drive mechanochemistry? A study of ZDDP tribofilm formation
Authors: Fang Lu , Korres Spyridon , Lamberti William A. , Webster Martin N. , Carpick Robert W.
Published: July 2022

The goal of the present work is to determine the independent effects of compressive stress, shear stress, and temperature on tribofilm-formation kinetics.
Protective effect of traditional Korean fermented soybean foods (doenjang) on a dextran sulfate sodium-induced colitis mouse model
Authors: Yang Hee-Jong , Jeong Su-Ji , Ryu Myeong Seon , Ha Gwangsu , Jeong Do-Youn , Park Young Mi , Lee Hak Yong , Bae Jun Sang
Published: July 2022

Doenjang had phytochemicals as the potent anti-inflammatory. Doenjang may reduce inflammatory factors in DSS-induced colitis in mice. Doenjang protects against UC and may be possibly use in developing functional foods.
Metals (Al, Fe, Zn) doped in single walled carbon nanotubes for catalytic oxidation of H2O to H2O2: a theoretical investigation
Authors: Chen Yang , Zhang Shuang , Mao Dan , Xie Rui-Fang , Qin Qiao-Qiao , Su Xin-Tong , Zhai Bin , Li Lai-Cai , Zheng Yan
Published: July 2022

A theoretical study on the reaction mechanism of oxygen reduction of metal (Al, Zn, Fe) supported carbon nanotubes to hydrogen peroxide.
Acid–base responsive photoluminescence switching of CdSe/ZnS quantum dots coupled to plasmonic gold film using nanometer-thick poly[(2-diethylamino)ethyl methacrylate] layer
Authors: Moradi Maryam , Staude Isabelle , Pertsch Thomas , Jäger Michael , Schubert Ulrich S.
Published: July 2022

Control of plasmon–nanoemitter interaction at nanoscale by a pH responsive intermediate layer of poly[(2-diethylamino)ethyl methacrylate] enables the modulation of photoluminescence intensity of nanoemitters, being an appealing switchable system.