bluuebunny/crossref_metadata_2025 huggingface dataset:
The sylvian keyhole approach for surgical clipping of middle cerebral artery aneurysms: Technical nuance to the minipterional craniotomy
Authors: Rychen Jonathan , Saemann Attill , Gehweiler Julian E. , Roethlisberger Michel , Soleman Jehuda , Hutter Gregor , Müller-Gerbl Magdalena , Mariani Luigi , Guzman Raphael
Published: December 2022

Background

The minipterional (MPT) craniotomy is a workhorse approach for clipping of middle cerebral artery (MCA) aneurysms. Because it aims to reach the skull base, traction on the temporal muscle is required. As a result, patients may suffer from transient postoperative temporal muscle discomfort. The sylvian keyhole approach (SKA) represents an alternative craniotomy for the clipping of MCA aneurysms. The aims of this study are to describe the operative technique of the SKA and to discuss the benefits and disadvantages compared to the MPT craniotomy.

Methods

In this technical note, we report the experience gained with the SKA. This experience was acquired with virtual reality, 3D-printed models, and anatomical dissections. We also present two clinical cases.

Results

The SKA is centered on the distal sylvian fissure and tailored toward the specific MCA aneurysm. Traction to the temporal muscle is not necessary because access to the skull base is not sought. With the SKA, dissection of the MCA is performed from distal to proximal, aiming for a proximal control at the level of the M1-segment. The limen insulae was identified as a key anatomical landmark for approach selection. The SKA offers good surgical maneuverability when the aneurysm is located at the level or distal to the limen. The MPT craniotomy, however, remains the most appropriate approach when the aneurysm is located proximal to the limen.

Conclusion

The SKA represents a feasible and innovative alternative approach to the MPT craniotomy for surgical clipping of unruptured MCA aneurysms located at the level or distal to the limen insulae.
Intra-osseous infiltration of adipose mesenchymal stromal cells and plasma rich in growth factors to treat acute full depth cartilage defects in a rabbit model: Serum osteoarthritis biomarkers and macroscopical assessment
Authors: Torres-Torrillas Marta , Damiá Elena , Peláez Pau , Miguel-Pastor Laura , Cuervo Belén , Cerón José J. , Carrillo José M. , Rubio Mónica , Sopena Joaquín J.
Published: December 2022

Introduction

Intra-articular infiltration of plasma rich in growth factors (PRGF) and adipose mesenchymal stromal cells (AMSCs) are known to inhibit osteoarthritis progression. However, in severely affected patients, the treatment cannot reach the deeper layers of the articular cartilage; thus, its potential is limited. To overcome this limitation, intra-osseous infiltrations have been suggested. The purpose of this study is to assess the impact of intra-osseous infiltration therapies on serum biomarkers of osteoarthritis and to assess cartilage regeneration macroscopically.

Materials and methods

A total of 80 rabbits were divided into four groups based on the intra-osseous treatment administered on the day of surgery: control, PRGF, AMSCs and a combination of PRGF + AMSCs. In addition, all groups received a single intra-articular administration of PRGF on the same day. Serum biomarker levels were measured before infiltration and 28-, 56-, and 84-days post infiltration, and macroscopical assessment was conducted at 56- and 84-days follow-up post infiltration.

Results

In the PRGF + AMSCs group, significantly lower concentrations of hyaluronic acid and type II collagen cleavage neoepitope were recorded at all time points during the study, followed by PRGF, AMSCs and control groups. Regarding macroscopical assessment, lower scores were obtained in PRGF + AMSCs group at all study times.

Discussion

The results suggest that the combination of intra-articular PRGF with intra-osseous PRGF or AMSCs achieves better results in rabbits with acute chondral defects and that intra-osseous infiltration is a safe procedure.
The Emerging Role of Tumor Microenvironmental Stimuli in Regulating Metabolic Rewiring of Liver Cancer Stem Cells
Authors: Correnti Margherita , Binatti Eleonora , Gammella Elena , Invernizzi Pietro , Recalcati Stefania
Published: December 2022

Primary liver cancer (PLC) is one of the most devastating cancers worldwide. Extensive phenotypical and functional heterogeneity is a cardinal hallmark of cancer, including PLC, and is related to the cancer stem cell (CSC) concept. CSCs are responsible for tumor growth, progression, relapse and resistance to conventional therapies. Metabolic reprogramming represents an emerging hallmark of cancer. Cancer cells, including CSCs, are very plastic and possess the dynamic ability to constantly shift between different metabolic states depending on various intrinsic and extrinsic stimuli, therefore amplifying the complexity of understanding tumor heterogeneity. Besides the well-known Warburg effect, several other metabolic pathways including lipids and iron metabolism are altered in PLC. An increasing number of studies supports the role of the surrounding tumor microenvironment (TME) in the metabolic control of liver CSCs. In this review, we discuss the complex metabolic rewiring affecting liver cancer cells and, in particular, liver CSCs. Moreover, we highlight the role of TME cellular and noncellular components in regulating liver CSC metabolic plasticity. Deciphering the specific mechanisms regulating liver CSC–TME metabolic interplay could be very helpful with respect to the development of more effective and innovative combinatorial therapies for PLC treatment.
Stereotactic MR-Guided Adaptive Radiotherapy for Pancreatic Tumors: Updated Results of the Montpellier Prospective Registry Study
Authors: Bordeau Karl , Michalet Morgan , Keskes Aïcha , Valdenaire Simon , Debuire Pierre , Cantaloube Marie , Cabaillé Morgane , Portales Fabienne , Draghici Roxana , Ychou Marc , Assenat Eric , Mazard Thibault , Samalin Emmanuelle , Gauthier Ludovic , Colombo Pierre-Emmanuel , Carrere Sebastien , Souche François-Régis , Aillères Norbert , Fenoglietto Pascal , Azria David , Riou Olivier
Published: December 2022

Introduction: Stereotactic MR-guided Adaptive RadioTherapy (SMART) is a novel process to treat pancreatic tumors. We present an update of the data from our prospective registry of SMART for pancreatic tumors. Materials and methods: After the establishment of the SMART indication in a multidisciplinary board, we included all patients treated for pancreatic tumors. Primary endpoints were acute and late toxicities. Secondary endpoints were survival outcomes (local control, overall survival, distant metastasis free survival) and dosimetric advantages of adaptive process on targets volumes and OAR. Results: We included seventy consecutive patients in our cohort between October 2019 and April 2022. The prescribed dose was 50 Gy in 5 consecutive fractions. No severe acute SMART related toxicity was noted. Acute and late Grade ≤ 2 gastro intestinal were low. Daily adaptation significantly improved PTV and GTV coverage as well as OAR sparing. With a median follow-up of 10.8 months since SMART completion, the median OS, 6-months OS, and 1-year OS were 20.9 months, 86.7% (95% CI: (75–93%), and 68.6% (95% CI: (53–80%), respectively, from SMART completion. Local control at 6 months, 1 year, and 2 years were, respectively, 96.8 % (95% CI: 88–99%), 86.5 (95% CI: 68–95%), and 80.7% (95% CI: 59–92%). There was no grade > 2 late toxicities. Locally Advanced Pancreatic Cancers (LAPC) and Borderline Resectable Pancreatic Cancers (BRPC) patients (52 patients) had a median OS, 6-months OS, and 1-year OS from SMART completion of 15.2 months, 84.4% (95% CI: (70–92%)), and 60.5% (95% CI: (42–75%)), respectively. The median OS, 1-year OS, and 2-year OS from initiation of induction chemotherapy were 22.3 months, 91% (95% CI: (78–97%)), and 45.8% (95% CI: (27–63%)), respectively. Twenty patients underwent surgical resection (38.7 % of patients with initially LAPC) with negative margins (R0). Conclusion: To our knowledge, this is the largest series of SMART for pancreatic tumors. The treatment was well tolerated with only low-grade toxicities. Long-term OS and LC rates were achieved. SMART achieved high secondary resection rates in LAPC patients.
Risk of Thromboembolic Events and Major Adverse Cardiovascular Events Following Antivascular Endothelial Growth Factor Therapy in Patients with Colorectal Cancer
Authors: Chen Yen-Cheng , Yeh Liang-Tsai , Yang Shun-Fa , Chou Ming-Chih , Huang Jing-Yang , Yeh Chao-Bin
Published: December 2022

Antivascular endothelial growth factor (anti-VEGF) therapy has been a standard treatment for patients with metastatic colorectal cancer. However, the risk of thromboembolic events and cardiovascular events associated with this therapy remains controversial. We assessed whether anti-VEGF therapy increases the risk of thromboembolic events or major adverse cardiovascular events (MACEs) in patients with colorectal cancer based on real-world evidence. This retrospective cohort study was designed using linked 2009–2016 nationwide databases, including the Taiwan Cancer Registry, the National Health Insurance Research Database, and Taiwan’s National Death Index. In total, 189,708 patients newly diagnosed as having advanced colorectal cancer from 2009 to 2016 were identified and categorized into the anti-VEGF and comparator groups through age, sex, clinical stage, and diagnosis date (within 180 days) matching. Propensity score matching was further performed to balance the baseline characteristics between the two groups. The Kaplan–Meier method was used to create the cumulative incidence curves of thromboembolic events and MACEs, and log-rank tests were used to compare the differences in Kaplan–Meier curves. Competing hazard ratios (HRs) for thromboembolic events and MACEs were estimated using the Fine–Gray method when considering the competing event of death. Statistical analysis was performed using two-tailed tests with a significance level of 0.05. In total, 4635 patients were included in both the anti-VEGF group and comparator group. The risk of thromboembolic events and MACEs did not differ significantly between the two groups. After propensity score matching, the adjusted HR for MACEs or thromboembolic events was 1.040, which for MACEs was 0.989, and that for thromboembolic events was 1.028. The competing HR for MACEs or thromboembolic events was 0.921, which for MACEs, was 0.862, and that for thromboembolic events was 0.908. In conclusion, patients with advanced colorectal cancer who received anti-VEGF therapy did not exhibit significantly higher risks of thromboembolic events and MACEs than those without anti-VEGF therapy. Our study provides real-world evidence regarding the safety of anti-VEGF therapy in Asian patients with advanced colorectal cancer.
Adiabatic Amplification of the Harmonic Oscillator Energy When the Frequency Passes through Zero
Authors: Dodonov Viktor V. , Dodonov Alexandre V.
Published: December 2022

We study the evolution of the energy of a harmonic oscillator when its frequency slowly varies with time and passes through a zero value. We consider both the classical and quantum descriptions of the system. We show that after a single frequency passage through a zero value, the famous adiabatic invariant ratio of energy to frequency (which does not hold for a zero frequency) is reestablished again, but with the proportionality coefficient dependent on the initial state. The dependence on the initial state disappears after averaging over the phases of initial states with the same energy (in particular, for the initial vacuum, the Fock and thermal quantum states). In this case, the mean proportionality coefficient is always greater than unity. The concrete value of the mean proportionality coefficient depends on the power index of the frequency dependence on a time near the zero point. In particular, the mean energy triplicates if the frequency tends to zero linearly. If the frequency attains zero more than once, the adiabatic proportionality coefficient strongly depends on the lengths of time intervals between zero points, so that the mean energy behavior becomes quasi-stochastic after many passages through a zero value. The original Born–Fock theorem does not work after the frequency passes through zero. However, its generalization is found: the initial Fock state becomes a wide superposition of many Fock states, whose weights do not depend on time in the new adiabatic regime. When the mean energy triplicates, the initial Nth Fock state becomes a superposition of, roughly speaking, 6N states, distributed nonuniformly. The initial vacuum and low-order Fock states become squeezed, as well as the initial thermal states with low values of the mean energy.
Resource Recovery and the Sherwood Plot
Authors: Karakatsanis Georgios , Makropoulos Christos
Published: December 2022

Our work analyzes the biophysical and economic foundations of the Sherwood Plot (SP). In general, the SP depicts the theoretical relationship between the cost of recovering a target material or an identified Value Added Compound (VAC) from a waste matrix and its dilution in the waste matrix; specifically suggesting that the recovery cost is reverse proportional to the VAC’s dilution in it. We further utilize the SP as a scientifically consistent and economically coherent analytical framework for measuring resource recovery performance. Initially, we analyze the SP’s fundamental physical properties, as well as its many potential economic extensions. Specifically, we substantiate the relation between a VAC’s Entropy, Dilution and Recovery Cost. On these grounds we present the SP’s remarkable and numerous economic properties that make it consistent to its physical foundations; thus integrating concisely its physical and economic aspects and postulate a generalized SP function. We further test econometrically the validity of an SP based on both deterministic and stochastic real data from a small-scale industrial unit of polyphenols’ recovery from natural fruit juice production residual wastewater. In turn, based on the fusion of our theoretical argumentation and empirical findings we dive into the epistemological extensions of the SP. Specifically, we study how the recovery cost structure at the single industry level is revealed by the SP and can be useful for postulating cost structure ontologies. Cost ontologies are in turn useful as a diagnostic of the formation process of VAC recovery markets as well as their structure and concentration, defining the industrial shares when many industries operate in the recovery of the same VAC.
Sequence Impedance Modeling and Optimization of MMC-HVDC Considering DC Voltage Control and Voltage Feedforward Control
Authors: Huang Tong , Chen Xin
Published: December 2022

The dynamic performance of the DC bus significantly influences the impedance characteristics of MMC and the system stability in a high-voltage direct current system. However, most of the existing MMC-HVDC system stability research simplifies the DC side as an ideal voltage source and ignores the impacts of voltage feedforward control, which affects the accuracy and practicability of stability analysis. In this paper, a sequence impedance model considering both DC voltage control and voltage feedforward control is developed, and the necessity of considering DC control and voltage feedforward control for MMC-HVDC stability analysis is illustrated. Then, the impact of control parameters on MMC-HVDC impedance is discussed, and the boundary conditions of control parameters are also derived. Finally, a method of control parameters design and impedance optimization for MMC-HVDC based on the stability boundary is proposed. Compared to the traditional optimization method, the system stability is further improved by the impedance optimization method proposed this paper.
Agnostic Battery Management System Capacity Estimation for Electric Vehicles
Authors: Calearo Lisa , Ziras Charalampos , Thingvad Andreas , Marinelli Mattia
Published: December 2022

Battery degradation is a main concern for electric vehicle (EV) users, and a reliable capacity estimation is of major importance. Every EV battery management system (BMS) provides a variety of information, including measured current and voltage, and estimated capacity of the battery. However, these estimations are not transparent and are manufacturer-specific, although measurement accuracy is unknown. This article uses extensive measurements from six diverse EVs to compare and assess capacity estimation with three different methods: (1) reading capacity estimation from the BMS through the central area network (CAN)-bus, (2) using an empirical capacity estimation (ECE) method with external current measurements, and (3) using the same method with measurements coming from the BMS. We show that the use of BMS current measurements provides consistent capacity estimation (a difference of approximately 1%) and can circumvent the need for costly experimental equipment and DC chargers. This data can simplify the ECE method only by using an on-board diagnostics port (OBDII) reader and an AC charger, as the car measures the current directly at the battery terminals.
Evaluation Metrics for Wind Power Forecasts: A Comprehensive Review and Statistical Analysis of Errors
Authors: Piotrowski Paweł , Rutyna Inajara , Baczyński Dariusz , Kopyt Marcin
Published: December 2022

Power generation forecasts for wind farms, especially with a short-term horizon, have been extensively researched due to the growing share of wind farms in total power generation. Detailed forecasts are necessary for the optimization of power systems of various sizes. This review and analytical paper is largely focused on a statistical analysis of forecasting errors based on more than one hundred papers on wind generation forecasts. Factors affecting the magnitude of forecasting errors are presented and discussed. Normalized root mean squared error (nRMSE) and normalized mean absolute error (nMAE) have been selected as the main error metrics considered here. A new and unique error dispersion factor (EDF) is proposed, being the ratio of nRMSE to nMAE. The variability of EDF depending on selected factors (size of wind farm, forecasting horizons, and class of forecasting method) has been examined. This is unique and original research, a novelty in studies on errors of power generation forecasts in wind farms. In addition, extensive quantitative and qualitative analyses have been conducted to assess the magnitude of forecasting error depending on selected factors (such as forecasting horizon, wind farm size, and a class of the forecasting method). Based on these analyses and a review of more than one hundred papers, a unique set of recommendations on the preferred content of papers addressing wind farm generation forecasts has been developed. These recommendations would make it possible to conduct very precise benchmarking meta-analyses of forecasting studies described in research papers and to develop valuable general conclusions concerning the analyzed phenomena.
Impact of X-Linked Hypophosphatemia on Muscle Symptoms
Authors: Romagnoli Cecilia , Iantomasi Teresa , Brandi Maria Luisa
Published: December 2022

X-linked hypophosphatemia (XLH) is the most common hereditary form of rickets and deficiency of renal tubular phosphate transport in humans. XLH is caused by the inactivation of mutations within the phosphate-regulating endopeptidase homolog X-linked (PHEX) gene and follows an X-dominant transmission. It has an estimated frequency of 1 case per 20,000, and over 300 distinct pathogenic variations have been reported that result in an excess of fibroblast growth factor 23 (FGF23) in the serum. Increased levels of FGF23 lead to renal phosphate loss, decreased serum 1,25-dihydroxyvitamin D, and increased metabolism of 1,25-dihydoxyvitamin D, resulting in hypophosphatemia. Major clinical manifestations include rickets, bone deformities, and growth retardation that develop during childhood, and osteomalacia-related fractures or pseudo-fractures, degenerative osteoarthritis, enthesopathy, dental anomalies, and hearing loss during adulthood, which can affect quality of life. In addition, fatigue is also a common symptom in patients with XLH, who experience decreased motion, muscle weakness, and pain, contributing to altered quality of life. The clinical and biomedical characteristics of XLH are extensively defined in bone tissue since skeletal deformations and mineralization defects are the most evident effects of high FGF23 and low serum phosphate levels. However, despite the muscular symptoms that XLH causes, very few reports are available on the effects of FGF23 and phosphate in muscle tissue. Given the close relationship between bones and skeletal muscles, studying the effects of FGF23 and phosphate on muscle could provide additional opportunities to understand the interactions between these two important compartments of the body. By describing the current literature on XLH and skeletal muscle dysfunctions, the purpose of this review is to highlight future areas of research that could contribute to a better understanding of XLH muscular disability and its management.
Molecular Characterization of Tropomyosin and Its Potential Involvement in Muscle Contraction in Pacific Abalone
Authors: Hanif Md Abu , Hossen Shaharior , Lee Won Kyo , Kho Kang Hee
Published: December 2022

Tropomyosin (TPM) is a contractile protein responsible for muscle contraction through its actin-binding activity. The complete sequence of TPM in Haliotis discus hannai (Hdh-TPM) was 2160 bp, encoding 284 amino acids, and contained a TPM signature motif and a TPM domain. Gene ontology (GO) analysis based on the amino acid sequence predicted Hdh-TPM to have an actin-binding function in the cytoskeleton. The 3D analysis predicted the Hdh-TPM to have a coiled-coil α-helical structure. Phylogenetically, Hdh-TPM formed a cluster with other TPM/TPM1 proteins during analysis. The tissue-specific mRNA expression analysis found the higher expression of Hdh-TPM in the heart and muscles; however, during embryonic and larval development (ELD), the higher expression was found in the trochophore larvae and veliger larvae. Hdh-TPM expression was upregulated in fast-growing abalone. Increasing thermal stress over a long period decreased Hdh-TPM expression. Long-term starvation (>1 week) reduced the mRNA expression of Hdh-TPM in muscle; however, the mRNA expression of Hdh-TPM was significantly higher in the mantle, which may indicate overexpression. This study is the first comprehensive study to characterize the Hdh-TPM gene in Pacific abalone and to report the expression of Hdh-TPM in different organs, and during ELD, different growth patterns, thermal stress, seasonal changes, and starvation.
Description of Stone Morphology and Crystalluria Improve Diagnosis and Care of Kidney Stone Formers
Authors: Letavernier Emmanuel , Bazin Dominique , Daudon Michel
Published: December 2022

Stone analysis by physical methods is critical to determine their chemical nature and to diagnose the underlying conditions affecting kidney stone formers. This analysis should be completed by a morphologic examination of stone surface and section, leading to the diagnosis of anatomical or metabolic disorders and of specific diseases. Crystalluria study, the analysis of urine crystals, provides complementary information and is extremely useful for both diagnosis and patient follow-up. This review describes briefly how these techniques may be used and in which conditions stone morphology and urine crystal description are particularly relevant for patients medical care.
Urban Agriculture in Thailand: Adoption Factors and Communication Guidelines to Promote Long-Term Practice
Authors: Sereenonchai Sukanya , Arunrat Noppol
Published: December 2022

The purpose of this study is to clarify influencing factors on the adoption and continuing practice of urban agriculture, and to propose communication guidelines to encourage more adoption and long-term practice. The psychological theories of the Theory of Reasoned Action (TRA), the Theory of Planned Behavior (TPB), and the Health Belief Model (HBM) were integrated to explain people’s behavior. Both quantitative and qualitative approaches were employed with 325 villagers of 13 communities in seven provinces of Thailand. The following techniques and instruments were used: a statistical analysis crosstab, stepwise multiple linear regression, one-way ANOVA, multinomial logistic regression, decision tree analysis, and descriptive content analysis using QDA lite miner software. The key results clearly show that attitude, perceived benefits, and perceived readiness were a significantly positive influence on those who adopted urban agriculture. Key drivers to villagers’ intention to continue practicing urban agriculture for the initiator group who own agricultural land were perceived to be behavioral control and social norm, while perceived readiness and communication played a crucial role for other groups to continue practicing urban agriculture. Communication guidelines to promote long-term urban agriculture practice can be designed based on the EAST framework, by making it easy, attractive, social, and timely, and through the Critical Participatory Action Research process.
Should I Stay or Should I Go (to the Office)?—Effects of Working from Home, Autonomy, and Core Self–Evaluations on Leader Health and Work–Life Balance
Authors: Neidlinger Stephanie Maren , Felfe Jörg , Schübbe Katharina
Published: December 2022

Leaders represent a high-demand group in organizations. The effects of leaders’ personal and workplace resources on their health and work–life balance have often slipped under the radar, as most studies are directed outwardly and focus on follower outcomes. With this study, we closed a gap in the research and investigated the positive effects of remote work, autonomy, and leaders’ core-self evaluations (CSE) on two important leader outcomes: health and work–life balance. We hypothesized that the relationship between remote work and the outcomes would be moderated by leaders’ CSE and their autonomy—in such a way that leaders with lower resources benefit more from remote work and achieve better health and work–life balance the more days they spend working from home. A sample of 367 leaders reported their frequency of working from home, their autonomy, and CSE. Their health and work–life balance were assessed five months later. Results showed a moderating effect of CSE on both outcomes, indicating that leaders with low CSE benefit more in terms of health and work–life balance. There was no moderating effect of autonomy. Leaders with high resources (autonomy and CSE) had overall higher levels of health and work–life balance regardless of work location. Practitioners in organizations should consider working from home as a resource for leaders, particularly if personal resources are lower.
The Mechanism of Cumulative Ecological Risk Affecting College Students’ Sense of Social Responsibility: The Double Fugue Effect of Belief in a Just World and Empathy
Authors: Yi Yiyu , Tan Qianbao , Liu Jiahui , Liang Fuqun , Liu Chao , Yin Zhenbiao
Published: December 2022

According to bioecological theory, the development of college students’ social responsibility is affected by the cumulative effect of ecological risks. However, research on the impact of cumulative ecological risk on social responsibility and its potential mechanisms are still lacking. Carol Gilligan (1982) proposed that the ethics of care and justice are like two related but independent melodies, which together constitute the whole of moral psychology. However, despite being an important part of moral psychology, social responsibility has rarely been investigated empirically with regards to the psychological mechanisms of justice and caring orientation. Therefore, the current study had 1607 college students complete questionnaires regarding cumulative ecological risk, social responsibility, belief in a just world, and empathy, aiming to explore the impact of cumulative ecological risk on college students’ sense of social responsibility and the mediating roles of belief in a just world and empathy. Results showed that: (1) cumulative ecological risk was significantly negatively correlated with college students’ sense of social responsibility, belief in a just world, and empathy, whereas social responsibility, belief in a just world, and empathy were significantly positively correlated; (2) belief in a just world and empathy played mediating roles in the relationship between cumulative ecological risk and social responsibility. The results also showed that the development of college students’ sense of social responsibility was affected by the cumulative ecological risk from various directions; this influence was also seen to play a role in the motivation system of social responsibility through the ethics of care with empathy as the important part, as well as through the ethics of justice. The results suggest that we should reduce the ecological risks at their source, and improve and consolidate students’ social support systems; moreover, we should not only enhance college students’ sense of mission and responsibility to consciously maintain social justice order, but also adopt empathy training as a part of the curriculum to improve students’ empathy at the individual level.
Research on the Spatial Correlation of China’s Haze Pollution and the Government’s Cooperative Governance Competitive Strategy
Authors: Wang Shijin , Ji Guirong , Hu Zhaolian , Qiu Fangdao
Published: December 2022

A government’s choice of environmental strategy plays an important role in the coordinated governance of regional air pollution. Based on changes in China’s environmental policies and on changes in environmental indicators over the years, this paper selects regional haze data from the years 2005, 2009, 2013, and 2017; uses social network analysis to describe the structural characteristics of a spatial correlation network in China; measures the level of coordination using a population gravity model; and further analyzes the influence of the overall structural characteristics of spatial networks on the level of coordination. The results show that the spatial association of regional haze presents a typical “central edge” network structure. The Beijing–Tianjin–Hebei region and the Yangtze River Delta region are the largest emitters in China. The coordination level of haze control in China showed a fluctuating upward trend, but the overall level of coordination is relatively low, and there is still great room for improvement. Based on the above characteristics, using the provincial panel data from 2005–2017, a two-zone spatial Durbin model was built to empirically test the impact of changes to the environmental performance assessment system on local coordinated haze-control decisions and their stage characteristics. The overall sample results show that there was a “race to the bottom” among Chinese provinces during the study period. When the haze control intensity in neighboring areas was relaxed, the regional governments also tended to relax their own environmental regulation intensity. The time-based analysis results further show that with the improvement of the environmental performance assessment system, the strategy selection of coordinated governmental haze-management presents the possibility of a “race to the top”.
Molecular Mechanisms Underlying Metabolic Resistance to Cyflumetofen and Bifenthrin in Tetranychus urticae Koch on Cowpea
Authors: Liu Zhenxiu , Wu Fuxing , Liang Weikang , Zhou Lijuan , Huang Jiguang
Published: December 2022

Tetranychus urticae Koch (T. urticae) is one of the most tremendous herbivores due to its polyphagous characteristics, and is resistant to most acaricides. In this study, enzyme-linked immunosorbent assay (ELISA), transcriptome sequencing (RNA-seq) and quantitative real-time PCR (qRT-PCR) were carried out to analyze the mechanisms of T. urticae metabolic resistance to cyflumetofen and bifenthrin on cowpea. The enzyme activity of UDP-glucuronosyltransferases (UGTs) and carboxylesterases (CarEs) in the cyflumetofen-resistant (R_cfm) strain significantly decreased, while that of cytochrome P450 monooxygenases (P450s) significantly increased. Meanwhile, the activities of glutathione-S-transferases (GSTs), CarEs and P450s in the bifenthrin-resistant (R_bft) strain were significantly higher than those in the susceptible strain (Lab_SS). According to the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses, in the R_cfm mite strain, two carboxyl/cholinesterase (CCE) genes and two P450 genes were upregulated and one gene was downregulated, namely CYP392E7; in the R_bft mite strain, eleven CCE, nine UGT, two P450, four GST and three ABC genes were upregulated, while four CCE and three P450 genes were downregulated. Additionally, 94 differentially expressed genes (DEGs) were common to the two resistant groups. Specifically, TuCCE46 and TuCCE70 were upregulated in both resistant groups. Furthermore, the qRT-PCR validation data were consistent with those from the transcriptome sequencing analysis. Specifically, TuCCE46 (3.37-fold) was significantly upregulated in the R_cfm strain, while in the R_bft strain, TeturUGT22 (5.29-fold), teturUGT58p (1.74-fold), CYP392A11 (2.89-fold) and TuGSTd15 (5.12-fold) were significantly upregulated and TuCCE01 (0.13-fold) and CYP392A2p (0.07-fold) were significantly downregulated. Our study indicates that TuCCE46 might play the most important role in resistance to cyflumetofen, and TuCCE01, teturUGT58p, teturUGT22, CYP392A11, TuGSTd15, TuGSTm09 and TuABCG-13 were prominent in the resistance to bifenthrin. These findings provide further insight into the critical genes involved in the metabolic resistance of T. urticae to cyflumetofen and bifenthrin.
New Affordable Methods for Large-Scale Isolation of Major Olive Secoiridoids and Systematic Comparative Study of Their Antiproliferative/Cytotoxic Effect on Multiple Cancer Cell Lines of Different Cancer Origins
Authors: Papakonstantinou Aikaterini , Koumarianou Petrina , Rigakou Aimilia , Diamantakos Panagiotis , Frakolaki Efseveia , Vassilaki Niki , Chavdoula Evangelia , Melliou Eleni , Magiatis Prokopios , Boleti Haralabia
Published: December 2022

Olive oil phenols (OOPs) are associated with the prevention of many human cancers. Some of these have been shown to inhibit cell proliferation and induce apoptosis. However, no systematic comparative study exists for all the investigated compounds under the same conditions, due to difficulties in their isolation or synthesis. Herein are presented innovative methods for large-scale selective extraction of six major secoiridoids from olive oil or leaves enabling their detailed investigation. The cytotoxic/antiproliferative bioactivity of these six compounds was evaluated on sixteen human cancer cell lines originating from eight different tissues. Cell viability with half-maximal effective concentrations (EC50) was evaluated after 72 h treatments. Antiproliferative and pro-apoptotic effects were also assessed for the most bioactive compounds (EC50 ≤ 50 μM). Oleocanthal (1) showed the strongest antiproliferative/cytotoxic activity in most cancer cell lines (EC50: 9–20 μM). The relative effectiveness of the six OOPs was: oleocanthal (1) > oleuropein aglycone (3a,b) > ligstroside aglycone (4a,b) > oleacein (2) > oleomissional (6a,b,c) > oleocanthalic acid (7). This is the first detailed study comparing the bioactivity of six OOPs in such a wide array of cancer cell lines, providing a reference for their relative antiproliferative/cytotoxic effect in the investigated cancers.
Favorable Biological Performance Regarding the Interaction between Gold Nanoparticles and Mesenchymal Stem Cells
Authors: Lin Ruei-Hong , Lee Hsu-Tung , Yeh Chun-An , Yang Yi-Chin , Shen Chiung-Chyi , Chang Kai-Bo , Liu Bai-Shuan , Hsieh Hsien-Hsu , Wang Hui-Min David , Hung Huey-Shan
Published: December 2022

Gold nanoparticles (AuNPs) are well known to interact with cells, leading to different cell behaviors such as cell proliferation and differentiation capacity. Biocompatibility and biological functions enhanced by nanomedicine are the most concerning factors in clinical approaches. In the present research, AuNP solutions were prepared at concentrations of 1.25, 2.5, 5 and 10 ppm for biocompatibility investigations. Ultraviolet–visible spectroscopy was applied to identify the presence of AuNPs under the various concentrations. Dynamic Light Scattering assay was used for the characterization of the size of the AuNPs. The shape of the AuNPs was observed through a Scanning Electron Microscope. Afterward, the mesenchymal stem cells (MSCs) were treated with a differentiation concentration of AuNP solutions in order to measure the biocompatibility of the nanoparticles. Our results demonstrate that AuNPs at 1.25 and 2.5 ppm could significantly enhance MSC proliferation, decrease reactive oxygen species (ROS) generation and attenuate platelet/monocyte activation. Furthermore, the MSC morphology was observed in the presence of filopodia and lamellipodia while being incubated with 1.25 and 2.5 ppm AuNPs, indicating that the adhesion ability was enhanced by the nanoparticles. The expression of matrix metalloproteinase (MMP-2/9) in MSCs was found to be more highly expressed under 1.25 and 2.5 ppm AuNP treatment, relating to better cell migrating ability. Additionally, the cell apoptosis of MSCs investigated with Annexin-V/PI double staining assay and the Fluorescence Activated Cell Sorting (FACS) method demonstrated the lower population of apoptotic cells in 1.25 and 2.5 ppm AuNP treatments, as compared to high concentrations of AuNPs. Additionally, results from a Western blotting assay explored the possibility that the anti-apoptotic proteins Cyclin-D1 and Bcl-2 were remarkably expressed. Meanwhile, real-time PCR analysis demonstrated that the 1.25 and 2.5 ppm AuNP solutions induced a lower expression of inflammatory cytokines (TNF-α, IL-1β, IFN-γ, IL-6 and IL-8). According to the tests performed on an animal model, AuNP 1.25 and 2.5 ppm treatments exhibited the better biocompatibility performance, including anti-inflammation and endothelialization. In brief, 1.25 and 2.5 ppm of AuNP solution was verified to strengthen the biological functions of MSCs, and thus suggests that AuNPs become the biocompatibility nanomedicine for regeneration research.
QTL and Candidate Genes: Techniques and Advancement in Abiotic Stress Resistance Breeding of Major Cereals
Authors: Raj Sujitra Raj Genga , Nadarajah Kalaivani
Published: December 2022

At least 75% of the world’s grain production comes from the three most important cereal crops: rice (Oryza sativa), wheat (Triticum aestivum), and maize (Zea mays). However, abiotic stressors such as heavy metal toxicity, salinity, low temperatures, and drought are all significant hazards to the growth and development of these grains. Quantitative trait locus (QTL) discovery and mapping have enhanced agricultural production and output by enabling plant breeders to better comprehend abiotic stress tolerance processes in cereals. Molecular markers and stable QTL are important for molecular breeding and candidate gene discovery, which may be utilized in transgenic or molecular introgression. Researchers can now study synteny between rice, maize, and wheat to gain a better understanding of the relationships between the QTL or genes that are important for a particular stress adaptation and phenotypic improvement in these cereals from analyzing reports on QTL and candidate genes. An overview of constitutive QTL, adaptive QTL, and significant stable multi-environment and multi-trait QTL is provided in this article as a solid framework for use and knowledge in genetic enhancement. Several QTL, such as DRO1 and Saltol, and other significant success cases are discussed in this review. We have highlighted techniques and advancements for abiotic stress tolerance breeding programs in cereals, the challenges encountered in introgressing beneficial QTL using traditional breeding techniques such as mutation breeding and marker-assisted selection (MAS), and the in roads made by new breeding methods such as genome-wide association studies (GWASs), the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system, and meta-QTL (MQTL) analysis. A combination of these conventional and modern breeding approaches can be used to apply the QTL and candidate gene information in genetic improvement of cereals against abiotic stresses.
Grapevine Leafroll-Associated Virus 3 in Single and Mixed Infections Triggers Changes in the Oxidative Balance of Four Grapevine Varieties
Authors: Hančević Katarina , Čarija Mate , Radić Brkanac Sandra , Gaši Emanuel , Likar Matevž , Zdunić Goran , Regvar Marjana , Radić Tomislav
Published: December 2022

With the aim to characterize changes caused by grapevine leafroll-associated virus 3 (GLRaV-3) singly or in coinfection with other viruses and to potentially determine genotype-specific or common markers of viral infection, thirty-six parameters, including nutrient status, oxidative stress parameters, and primary metabolism as well as symptoms incidence were investigated in ‘Cabernet Franc,’ ‘Merlot,’ ‘Pinot Noir,’ and ‘Tribidrag’ grapevine varieties. Host responses were characterized by changes in cellular redox state rather than disturbances in nutrient status and primary metabolic processes. Superoxide dismutase, hydrogen peroxide, and proteins were drastically affected regardless of the type of isolate, the host, and the duration of the infection, so they present cellular markers of viral infection. No clear biological pattern could be ascertained for each of the GLRaV-3 genotypes. There is a need to provide a greater understanding of virus epidemiology in viticulture due to the increasing natural disasters and climate change to provide for global food production security. Finding grape varieties that will be able to cope with those changes can aid in this task. Among the studied grapevine varieties, autochthonous ‘Tribidrag’ seems to be more tolerant to symptoms development despite numerous physiological changes caused by viruses.
Tissue Engineering for Gastrointestinal and Genitourinary Tracts
Authors: Elia Elissa , Brownell David , Chabaud Stéphane , Bolduc Stéphane
Published: December 2022

The gastrointestinal and genitourinary tracts share several similarities. Primarily, these tissues are composed of hollow structures lined by an epithelium through which materials need to flow with the help of peristalsis brought by muscle contraction. In the case of the gastrointestinal tract, solid or liquid food must circulate to be digested and absorbed and the waste products eliminated. In the case of the urinary tract, the urine produced by the kidneys must flow to the bladder, where it is stored until its elimination from the body. Finally, in the case of the vagina, it must allow the evacuation of blood during menstruation, accommodate the male sexual organ during coitus, and is the natural way to birth a child. The present review describes the anatomy, pathologies, and treatments of such organs, emphasizing tissue engineering strategies.
Orientational Preferences of GPI-Anchored Ly6/uPAR Proteins
Authors: Zaigraev Maxim M. , Lyukmanova Ekaterina N. , Paramonov Alexander S. , Shenkarev Zakhar O. , Chugunov Anton O.
Published: December 2022

Ly6/uPAR proteins regulate many essential functions in the nervous and immune systems and epithelium. Most of these proteins contain single β-structural LU domains with three protruding loops and are glycosylphosphatidylinositol (GPI)-anchored to a membrane. The GPI-anchor role is currently poorly studied. Here, we investigated the positional and orientational preferences of six GPI-anchored proteins in the receptor-unbound state by molecular dynamics simulations. Regardless of the linker length between the LU domain and GPI-anchor, the proteins interacted with the membrane by polypeptide parts and N-/O-glycans. Lynx1, Lynx2, Lypd6B, and Ly6H contacted the membrane by the loop regions responsible for interactions with nicotinic acetylcholine receptors, while Lypd6 and CD59 demonstrated unique orientations with accessible receptor-binding sites. Thus, GPI-anchoring does not guarantee an optimal ‘pre-orientation’ of the LU domain for the receptor interaction.
Direct Inhibition of SARS-CoV-2 Spike Protein by Peracetic Acid
Authors: Yamamoto Yuichiro , Nakano Yoshio , Murae Mana , Shimizu Yoshimi , Sakai Shota , Ogawa Motohiko , Mizukami Tomoharu , Inoue Tetsuya , Onodera Taishi , Takahashi Yoshimasa , Wakita Takaji , Fukasawa Masayoshi , Miyazaki Satoru , Noguchi Kohji
Published: December 2022

Peracetic acid (PAA) disinfectants are effective against a wide range of pathogenic microorganisms, including bacteria, fungi, and viruses. Several studies have shown the efficacy of PAA against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); however, its efficacy in SARS-CoV-2 variants and the molecular mechanism of action of PAA against SARS-CoV-2 have not been investigated. SARS-CoV-2 infection depends on the recognition and binding of the cell receptor angiotensin-converting enzyme 2 (ACE2) via the receptor-binding domain (RBD) of the spike protein. Here, we demonstrated that PAA effectively suppressed pseudotyped virus infection in the Wuhan type and variants, including Delta and Omicron. Similarly, PAA reduced the authentic viral load of SARS-CoV-2. Computational analysis suggested that the hydroxyl radicals produced by PAA cleave the disulfide bridges in the RBD. Additionally, the PAA treatment decreased the abundance of the Wuhan- and variant-type spike proteins. Enzyme-linked immunosorbent assay showed direct inhibition of RBD-ACE2 interactions by PAA. In conclusion, the PAA treatment suppressed SARS-CoV-2 infection, which was dependent on the inhibition of the interaction between the spike RBD and ACE2 by inducing spike protein destabilization. Our findings provide evidence of a potent disinfection strategy against SARS-CoV-2.
New Insights into the Crystal Chemistry of FeB-Type Compounds: The Case of CeGe
Authors: Freccero Riccardo , Frick Emmelina , Wilthorn Caroline , Hübner Julia-Maria
Published: December 2022

Several alkaline earth or rare earth binary monosilicides and -germanides possess complex bonding properties, such as polycation formation exceeding the scope of classical electron counting rules. In this study, we present characterization by powder and single-crystal diffraction and thermal analysis of CeGe, one of the few monogermanides crystallizing in the FeB-type structure. Comparative computational investigations for structure types experimentally observed for monogermanides and alternative structures with different structural motifs were performed to gain energetical insights into this family of compounds, underlining the preference for infinite germanium chains over other structural motifs. Formation enthalpy calculations and structural chemical analysis highlight the special position of FeB-type compounds among the monogermanides.
Finite Element Modeling of Hot Rolling of 1075 Carbon Steel Process with Variable Cross Section
Authors: Montemayor-de la Garza Karina , Zambrano-Robledo Patricia del Carmen , Zapata-Hernandez Oscar Jesus , Leduc-Lezama Luis Adolfo
Published: December 2022

Currently, it is common to use steel poles for applications in livestock and agriculture. In this work, finite element analysis of five hot rolling passes for the manufacture of farm poles using 1075 carbon steels from recycled railway material was developed. The steel industry in Mexico imports products from other countries or from companies specialized in metallurgy at an excessive cost. To be more competitive and save costs, companies seek the reutilization of existing resources such as the railway 1075 steel, which has good mechanical properties. SFTC DEFORM-3D software was used to model five hot rolling passes considering a variable cross section railway profile. The effect of rolling speed and temperature were considered to analyze flow behavior. Rolling loads were also determined.